LOCUS       DATNOI010000001         3041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1246889,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000001 DATNOI010000000
VERSION     DATNOI010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246889"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1050
                     /gene="trxB"
                     /locus_tag="VN968_00005"
     CDS             85..1050
                     /gene="trxB"
                     /locus_tag="VN968_00005"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HXL27665.1"
                     /translation="MPAPIHAKVVIIGSGPAGYTAAIYAARAMLEPVLIQGIQPGGQL
                     TITTDVENYPGFADVIQGPWLMEQMEKQAAHVGTRIVTDLVTKLELAHRPFRLTCDSG
                     DVYLAETVILATGAQARWLGTPSEEKFKGFGVSACATCDGFFYRGKEVMVVGGGNTAV
                     EEALFLTNFASQVTVVHRRDHFRAERIMQERLFKNPKIKVVWDCAIDEICGTDNPGKV
                     THVRLKNVKTGARTELPADGVFIAIGHAPATELILGQIKLKPSGYVEVAPNSTATSVP
                     GVFAAGDVADETYRQAVTAAGLGCMAALEAERFLALRASDRAAAE"
     gene            1053..1973
                     /locus_tag="VN968_00010"
     CDS             1053..1973
                     /locus_tag="VN968_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL27666.1"
                     /translation="MARTRNGSDMDWDKLKVFHAAAEAGSFTHAGEQLGLSQSAVSRQ
                     VSALEQELSVSLFHRHARGLILTEQGVLLFRTAHDVFMQLQAARAKLTDSRERPSGDL
                     KIQTPPALGINWLIPRLDEFTALYPDIRISLIVTDEDLDLSMREADVAIRTRKPTQPD
                     LIQRKLFSIGFHAYCSPEYIKRFGTPRTLDDLDSHRIAMLSDTQVQPHLQNRSWLIDA
                     GRNGSGPREAFFRVNNILGLVRACQQGLGIAALPDYLIEENTRLVQLFGESDSIQLDT
                     YFVYPEELKTVARVQVFRDFVVSKAQRWPS"
     gene            2445..>3041
                     /locus_tag="VN968_00015"
     CDS             2445..>3041
                     /locus_tag="VN968_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXL27667.1"
                     /translation="MTDLRASDLRTSDLQASDLQASLHGLWLPLVTPFRNGELDETSL
                     RRLARHYAALPVNGLILAATTGESLTLTPEETERLVFAVRDEISRTRNLPIRLGLSGS
                     NTRALLDTLDRTAAWPIDGYLISCPYYSRPSQQGLQRHFSALADRARHPVMLYNIPYR
                     TGVNLGNEAMLRLADHPDIVGLKDCCDDREQSLELLRRR"
BASE COUNT          564 a         1002 c          893 g          582 t
ORIGIN      
        1 acactttcgg cgccatcgcg aagccttccc gggcgcattc cgctcaagcg ctgaccaaat
       61 tcccttaaga gtgggatctg ctcgatgcct gcccctatcc atgccaaggt cgtcatcatt
      121 ggttccggcc cggctggcta caccgccgcg atctatgctg cccgcgcgat gctggaaccc
      181 gtcttgattc aaggcatcca gcccggcggc cagctcacca tcaccactga cgtggaaaac
      241 tatccgggtt tcgccgacgt catccagggc ccatggctga tggagcagat ggaaaagcag
      301 gccgcccatg tcggcaccag gatcgtcacc gacctcgtca ccaagcttga gctcgcccat
      361 cggccgttcc gcctgacctg cgacagcggc gacgtctatc tcgccgaaac cgtcattctc
      421 gccaccggcg cccaggcgcg ctggctcggc acgccgtcgg aagaaaaatt caagggtttt
      481 ggcgtctcgg cctgcgccac ctgcgacgga tttttctatc gcggcaagga agtcatggtg
      541 gtcggcggcg gcaacaccgc ggtcgaggaa gcgctgtttc tgaccaactt tgcgtctcaa
      601 gtcaccgtcg tgcatcgccg cgatcacttc cgcgccgagc gcatcatgca ggagcgcctg
      661 ttcaagaacc ccaagatcaa ggtggtgtgg gactgcgcca tcgacgaaat ctgcggcacc
      721 gacaaccccg gcaaggtcac gcatgtccgg ctgaagaatg tcaagaccgg cgcgcggacc
      781 gaactgccgg cggacggcgt cttcatcgcg atcggacacg cgcccgcaac cgagctgatc
      841 ctcggccaga tcaagctgaa gccatccggg tatgtcgagg tagcgccgaa ctcgaccgcg
      901 acctcggtgc ccggcgtgtt cgccgccggc gacgtcgccg acgaaaccta ccggcaggca
      961 gtcacggccg caggccttgg ctgcatggcc gccctcgagg ccgaacgctt tctcgctctg
     1021 cgcgcgagtg atcgcgcggc ggcggagtag tcatggctcg aacgcgcaac ggaagcgata
     1081 tggattggga caagctgaaa gtatttcacg cggcggcgga agcgggcagc ttcacccatg
     1141 ccggcgagca gctaggtctg tcgcaatcgg cggtgtcacg gcaggtcagc gcgctggagc
     1201 aggagctctc ggtgtcgctg ttccatcgcc atgcgcgcgg tctcattctt accgagcagg
     1261 gcgtcctgtt gtttcgcacc gcgcatgacg tgttcatgca gctgcaggcg gcgcgcgcca
     1321 agctgaccga cagccgcgag cggccaagcg gcgatctcaa gatccagacc ccgccggcgc
     1381 tgggcatcaa ctggctgatt ccgcggctcg acgaatttac cgcactttat ccggatattc
     1441 gtatctcgct gatcgtcacc gacgaagacc tcgatctgtc gatgcgcgag gccgacgtcg
     1501 cgatccgcac ccgcaagccg acccagccgg acctgatcca gcgcaagctg ttctcgatcg
     1561 gcttccatgc ctattgctcg ccggaataca tcaagcgctt cggcacgccg cgcaccctcg
     1621 acgacctcga ctcgcatcgc atcgccatgc tgagtgatac acaggttcaa ccgcacttgc
     1681 agaaccgcag ctggctgatc gatgccggcc gcaacggctc aggtccgcgc gaggccttct
     1741 tcagggtcaa caacatcctc ggcctggtcc gcgcctgcca gcagggattg ggtatcgcgg
     1801 cgctgccgga ctatctgatc gaggaaaata cccgcctggt gcagctgttc ggcgaatcgg
     1861 attcgatcca gctcgatacc tatttcgtct atcccgaaga gttgaagacg gtggcgcggg
     1921 tgcaggtatt ccgcgacttt gtggtgagca aggcgcagcg ctggccgtcc tgatcagcgg
     1981 agttggcggc ccccggtctc cgcatgactg acatgcggtg cgggcgcttg ctgcaaggag
     2041 ccaacgcgga tcataatggc tgcacgctga acgttcgtgt cgcgcatttg tccccctcct
     2101 ccagtggcgc ggaagttcag taatccctct tggaaggtga ttgtgtcggc ctcacgtggc
     2161 caatacctta ggccgggctc tttcgagccc ggcttttttt tgcggcaacg caacgtcacg
     2221 cgctgtgtcg tgattccgac gttgacagac tgggcgtcgc caatcatcat tcccgaatga
     2281 tcaccaatat gtgcatcgca gcgccgtcga aaaaagctcc ccttgcgggg actggagatc
     2341 gacgcgcgcg atagatttcg ccaatatcga tccgccaaaa ccccgccgtc tggacggggt
     2401 tttttattgt cccgtctccg gctcaacctc aaggagatgg accaatgact gaccttcgag
     2461 cttccgatct tcgaacttcc gatcttcaag cttccgatct tcaagcttcc ttgcacggcc
     2521 tctggctgcc gctggtcacg ccgttccgga atggcgagct cgacgagaca tcgctgcggc
     2581 ggctagcgcg gcattacgcg gcgcttcccg tcaatggatt gattctcgcc gccaccacgg
     2641 gcgagagcct cacactcaca cctgaggaaa ccgaacggct tgtattcgcg gtgcgggacg
     2701 aaatcagcag gaccaggaat cttccgatcc gcctcggcct gtccggcagc aacacacgcg
     2761 cgttgctgga cacactggac agaaccgcgg cgtggccgat cgacggatac ctgatttcct
     2821 gcccgtatta ctcgcggcct tcacagcagg gcttgcagcg gcacttcagt gcgctcgccg
     2881 atcgcgcgcg ccaccccgtc atgctctaca acattcccta ccggactggc gtcaatctcg
     2941 gcaatgaggc gatgctgcgg ttggccgatc atcccgacat cgtcggactc aaggattgct
     3001 gtgatgaccg cgagcaatcg ctcgaactcc tgcgccggcg c
//
LOCUS       DATNOI010000002         2404 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2321528,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000002 DATNOI010000000
VERSION     DATNOI010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2321528"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VN968_00020"
     CDS             complement(<1..408)
                     /locus_tag="VN968_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXL27668.1"
                     /translation="MLRAEDNKFLTESGAGTAMGELLRRFWLPVLLSEELPEADGVPK
                     KIVVMGEELLAFRDSRGVVGVIDQYCPHRGANLWLGRNEECGIRCVYHGWKFDTEGRC
                     VDMPTSYPDLKAKDLIRIKSYPVREWGDMIWAYM"
     gene            complement(481..1362)
                     /locus_tag="VN968_00025"
     CDS             complement(481..1362)
                     /locus_tag="VN968_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HXL27669.1"
                     /translation="MSSLASKQGPRYRHIADETEPYETIGVEKLTPIIGAEISGVDLA
                     GALSNRTMDEIHRALAENLVIFFRDQHITPEQHLAFGRQFGELHIHPAAPHEGDDPAL
                     MKIYADKDSPRANGEGWHSDVSCDVEPPMGSILHIRQCPPRGGDTLFASMYAAYESLS
                     ERMKTYLDGLTALHDGESTYRGLYANYGVADKPAYPRAEHPVVRTHPVTGKKALYVNR
                     GFTRHIVGIPSDESHAILNYLYQHAENPLFQCRFRWTENAIAFWDNRSTQHRAMWDYW
                     PHTRAGTRVTVKGERPV"
     gene            complement(1469..>2404)
                     /locus_tag="VN968_00030"
     CDS             complement(1469..>2404)
                     /locus_tag="VN968_00030"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL27670.1"
                     /translation="TAAVGRPVEIGTMNAVLIGTGIGLFEEFYVQSPRGNWIRAMHPL
                     RAISIYVVVVVALYFVALHVTHLLLGRLDDLPVVYRRLPYGLAFFTTFSVAGILMMRV
                     THFIGLRTLFDLLVGTYHRPVQERKILLFLDINGSTALGETLGALAVRAFVRKFLSDV
                     SQPIVDHGGEIYLYKGDGLVAIWDWANAIKGDAVLKAVDAMFAVVATERSAYERSFGT
                     APGFRIGIHGGDVIVSEQGDTKRSIGIYGDTINIAARMEEAARTHGVRCVISEAVAGA
                     LADRGCIRKIGADRVKGISTPILICEYVPNAGEAS"
BASE COUNT          450 a          736 c          759 g          459 t
ORIGIN      
        1 catataggcc cagatcatgt cgccccattc ccgcaccgga taggatttga tgcggatcag
       61 gtccttggcc ttgagatcgg gataggaggt cggcatgtcg acgcaacggc cctcggtgtc
      121 gaatttccag ccgtgataga cgcagcggat gccgcattcc tcgttgcggc cgagccagag
      181 attggcgccg cggtgcggac aatattgatc gatgacgccg accacgccgc gtgaatcgcg
      241 gaacgccagc agctcctcgc ccatcacgac gatctttttc ggcaccccgt cggcttccgg
      301 caattcctcc gacagcaaca ccggcagcca gaaccggcgc agcaattcgc ccatcgcggt
      361 acccgcaccg ctttcggtca ggaatttatt gtcctcggcg cggagcatgg ggatccccag
      421 ggttttgttt tgctttttga ccaagcttgg cgcaggcgaa gccgggcgtc aacgcgtgtt
      481 ctaaaccggc cgttcgccct tcaccgtcac gcgcgtgccg gcgcgggtgt gcggccagta
      541 gtcccacatc gcgcggtgct gggtgctgcg gttgtcccag aaggcgatgg cgttttcggt
      601 ccagcggaag cggcactgga acagcggatt ttccgcgtgc tggtagagat agttcaatat
      661 cgcgtggctc tcgtcgctgg ggatgccgac gatgtggcgg gtaaagccgc ggttgacata
      721 gagcgccttc ttgcccgtca ccggatgggt acgcaccacc gggtgctcgg cgcgcggata
      781 ggctggcttg tcggcaacgc cgtagttcgc atagaggccg cgataggtgg attcgccgtc
      841 gtgcagcgcc gtcagcccgt cgagataggt cttcatccgc tccgacagcg attcataggc
      901 ggcatacatg ctggcgaaca gcgtgtcgcc gccgcgcggc gggcattgcc ggatatggag
      961 gatggagccc atcggcggct cgacgtcgca ggagacgtcc gaatgccagc cctcgccgtt
     1021 ggcgcgcggc gaatccttgt cggcatagat cttcatcagt gcgggatcgt cgccctcatg
     1081 gggagcggcg ggatggatgt gcagctcgcc gaactggcgc ccaaaggcga gatgctgttc
     1141 tggggtgatg tgctgatcgc ggaagaagat cacgaggttc tcggcgagcg cgcggtggat
     1201 ctcgtccatg gtccggttgg acagcgcgcc ggcaagatcg acgcccgaga tttccgcgcc
     1261 gatgatcggg gtcagcttct cgaccccgat ggtctcgtaa ggttcggttt cgtcggcgat
     1321 gtgccgatag cgcggcccct gcttgctggc gagcgaactc atggcggtcc tccggtggcc
     1381 tgttgatttc cggccatcgt agcccggatg cggcgaaccg caatccgcaa agtgtgcggt
     1441 cagccccgtc agccgactcg gacttcgctt acgacgcttc gcccgcgttc ggaacatatt
     1501 cgcaaatcag gatcggcgtg gaaatgccct tcacacggtc cgcgccaatc ttgcgaatgc
     1561 agccgcggtc cgcaagagca ccagccacgg cttcggagat gacacagcgc acgccgtgcg
     1621 ttcgtgcagc ttcttccatg cgcgcggcga tgttgatcgt gtcgccataa atgccgatcg
     1681 agcgcttggt gtcgccctgc tcgctaacga tcacgtcgcc gccatgaata ccgatccgaa
     1741 agcccggcgc ggtgccgaac gagcgctcgt atgcgcttcg ctcagtcgct accacagcga
     1801 acatcgcatc gactgccttc aggactgcgt cacccttgat ggcgttggcc cagtcccaga
     1861 tcgcgaccag cccgtcgccc ttgtaaagat agatttctcc gccgtggtcg acgatgggtt
     1921 gcgatacatc ggataggaat ttgcgaacga acgcacgaac ggccagcgcc ccgagggtct
     1981 cgccaagcgc ggttgaaccg ttgatgtcga gaaacaacag aattttgcgt tcctgaacgg
     2041 gacgatgata ggtgccgacg agcagatcga acagggtgcg caatccgatg aagtgggtca
     2101 cccgcatcat caggatgccc gctaccgaaa atgtcgtgaa gaaggccagc ccatagggca
     2161 gtcgccggta gaccacgggc agatcgtcga ggcgtccaag cagcaaatga gtgacgtgca
     2221 gcgccacgaa atacagcgcc acaaccacca cgacgtagat ggaaatggcg cgtagcgggt
     2281 gcatggcgcg tatccagttg cccctcggac tctggacata gaattcctcg aacaacccaa
     2341 ttccggttcc gatcagaacc gcgttcatcg tgccgatctc gaccggccgc ccgacggctg
     2401 ccgt
//
LOCUS       DATNOI010000003         9205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2317092,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000003 DATNOI010000000
VERSION     DATNOI010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2317092"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VN968_00035"
     CDS             <1..814
                     /locus_tag="VN968_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXL27671.1"
                     /translation="EMTRDPTLDAFMAVGPLDSKITIDAIAATAAAKGEPKFLPVDVS
                     EAIAQKHRLYESEEIPGSTFSSSPARPEDKVETVSVNHLIIAPKTLSDTTIGTFVRQL
                     FAVRQTLARELPSAAKIEKPDTDKDAALPAHQGAAAYIDGTERTFLERYSDYFWAAIL
                     LLSGLGSAGAWLRQYSKRDEKEQSTLHRDKLLAMISKVRQAESSEELTAMQCEVDGVL
                     REALESYDDGAIEEGDLSAIGLVLEQFHHAVADRRIAMVGVSTPDLARRRAL"
     gene            1022..2578
                     /locus_tag="VN968_00040"
     CDS             1022..2578
                     /locus_tag="VN968_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HXL27672.1"
                     /translation="MRLKTILLGVAVVAVSFFVSLKAMDWLSPRSTVPAPVLVELPPL
                     PPAPRSSSIVAPIVITLTTIRDAADRGAPRTFAGKADNPVAQILQNTDIGWTASRGPI
                     SATGAQDVLSLATPLTGTLNVTGSLSAKTTGAIGDALGSLLGGDAAKRIGSVNIKSLN
                     AHAEIKGNVTITARPKLAAAWRIEPNLTAQVTLSDTNLSVSGARVNVPAQVKPLIDKT
                     VADQLDALQARIRNDPAFENNAKLQWAKACRSIPLQGPGTASSLPALWLELRPTRAIA
                     AQPRIDAATVTLMLGIEAETRITPAQTKPNCPFPATIDIVPPGPSRITIGVPIDLPFT
                     DINKIVEAQFTGRSFPEDGSGSVDVTVKRASVAASGDRLLISLLVKAKEKKSFVGLGG
                     EANIHIWGKPVLDQEQQTLRLTDIELAVESEAALGLLGAAARAAVPHLQKVLADKATI
                     DLKPFASNAQNKIAALISDFQKNEDGVRIAAEITSLRLADIAFDSKTLRVIAEAAGAI
                     NVSITALPGL"
     gene            2611..3720
                     /locus_tag="VN968_00045"
     CDS             2611..3720
                     /locus_tag="VN968_00045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXL27673.1"
                     /translation="MRERAAGIAHAWRALLTTLPFAAKQEWSWRSEADMQRLSLLLAA
                     IGLCVVLLVSGCGDNKQPAASAPPPAVTVVKVTATEIKPTTTFTGRIEAKNKVDLRAR
                     VDGFLEKRLFAEGADVAESDLLFVIEKGLYQAAVDQAKAALETAESTLKLADLDVDRQ
                     TQLFQRNVTAQATLEQATAKQGEARGNMLAQKAALEKAELQLSYTDIKSPIPGRIGRA
                     SVPVGNFVSPSNGALATVVSQDPIYASFPVTQREMLAVRKQQAEGKGSEYVIYVQLAD
                     GSRYPGAGKIDFLDNTVNQGTDTVQVRAAFANPDRVLVDGQLVTVVAEEGKGESALVV
                     PQQALQVDQTGPYVLVVDKDNKIQIRRVETDVARG"
     gene            3837..6995
                     /locus_tag="VN968_00050"
     CDS             3837..6995
                     /locus_tag="VN968_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017271107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HXL27674.1"
                     /translation="MLSSIFIDRPRLAVVISIVITVAGLVALFQIPIAQFPNIVPPQI
                     AVTASYAGASAEVVEATVAQPIEARVIGVDNMLYMKSTSGNDGSYTLNITFAVGTDPD
                     LNTVKVQNRVGLAEAQLPAEVRAQGISVTKKSSALLQVIALTSPDGRYDQLFLSNYAT
                     INVIDQLKRVPGVGDVSLFSPSDYSMKVWLNTDRLTSFGLVPGDIANALKAQNIQAAV
                     GRIGAQPALPDQQFQLNIQSKGRLANVEEFGAVVVRANPDGSLVRVRDVARVELAARL
                     SESLGRLDGQPAAVIGIYQSPGGNALDSAQRIGKILQELKTSFPEGVDYRITYDTTIF
                     VKESIKGVLHTLVEAFVLVVIVVFLFLGSVRATLIPLLAVPVALIGTFAVMLALGYSA
                     NTVSLLALVLAIGIVVDDAIVVVEAAEAQLEKDPSLSPAEASRRAMAEITAPIIAITL
                     VLLSVFVPVAFIPGISGELFRQFAVAVSVSMVISAINALTLSPALCAVFLSASHGPRR
                     GLIGYVLRGIDKARDGYSLVVHKTVRFAVYSLLVLVAVIAGAGWLFKVTPTGFLPSED
                     QGAVFGEIQLPEGASVNRTAAVTKRVEEIARNTPGIASVTSVIGYSLIDGLTKSNSAL
                     LILALNPFDERKTAPVSANGIIAGLMGQFQGIPEAIVFAYNLPPIIGLGTGSGFEFQL
                     QSQSGATPAEIAAVARGMVFAANQDATLRRVFTTYSANTPQLYLDIDREKVQTLGVNV
                     ADVFNALQSVLGSAYINDFNLYGRTWQVNIQGEASERARVDDIYRINVRNRNGDMVSI
                     RAFAAARLILGPHSIIRYNNFRSVTLNGGPAPGYSSGDAIAAMEKISAATLPRGYGYE
                     WTGTALQEKEASGKTTTILALAVLFAYLFLVGLYESWSIPVPVLLSISVGVLGSMAGL
                     LAFGLDNNLYAQIGLVVLIALAAKNGILIVEFAKERREQGLPIEQAAVEGARERFRAV
                     MMTSFAFIAGLVPLVIATGAGQLSRRGVGTAVFSGMIAASFLGVFLIPLLYAVFQHLR
                     ERIKRQPAKRDLQADAVAKPDH"
     gene            complement(7395..8237)
                     /locus_tag="VN968_00055"
     CDS             complement(7395..8237)
                     /locus_tag="VN968_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HXL27675.1"
                     /translation="MKTITAIAANKLGNFLTRDFRQIFGSTCDDMAERIGSAARSTIE
                     CLARSDALYHNYEHTLQVTMVGRDMLHGMMVHERIEPDDYGHLIAACLFHDIGYVRGV
                     LSGDTETEFIIDPDGTKIVLPRGASDAALAPYHVDRSKLFVFERFENSPILDPARVAA
                     AIEMTRFPVPEDRAEASGLEPKLVQASDFIGQLGDPLYSRKANALYWEFEEIGTNRQL
                     GYSSPADIVERYPAFFWDSVSMHLDDGIKYLNMTASGRQWIANLHHHILCAERAQRLM
                     GPQS"
     gene            complement(8449..8661)
                     /locus_tag="VN968_00060"
     CDS             complement(8449..8661)
                     /locus_tag="VN968_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HXL27676.1"
                     /translation="MTESVYKVIELIGTSKDSWEKAAQAAVDRAGKSLRELRVAEVVK
                     LDLQLDTKGKIEAYRAKLNVSFKFED"
     gene            8869..>9205
                     /locus_tag="VN968_00065"
     CDS             8869..>9205
                     /locus_tag="VN968_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL27677.1"
                     /translation="MKSIAAAVAASFVLAGSGTAANAQISDDVVKIGALTDMSSLYAD
                     ATGKGSLAAVQMAVADYGAKVKGKPVEVVSADHQNKPDIGVGIARNWYDNEKVDAIFD
                     VPTSSVALPV"
BASE COUNT         1776 a         2871 c         2808 g         1750 t
ORIGIN      
        1 cgagatgacc cgcgatccga ccctcgatgc gttcatggcc gtgggcccgc tggacagcaa
       61 gatcaccatc gacgccattg ccgcgaccgc ggctgccaag ggtgaaccca aatttctccc
      121 ggtcgatgtg tcggaggcga ttgcccagaa acaccggctc tacgaatcag aggaaattcc
      181 cggcagcacc ttcagctcct cgccggccag accggaagac aaagtcgaga cggtcagcgt
      241 caaccacctg atcatcgcgc caaagacttt gtccgacacg acgatcggca cgttcgtccg
      301 gcaattgttt gcggttcgtc agacgctcgc cagggaattg ccgagtgccg caaaaattga
      361 aaaaccggac accgacaagg acgcggcatt gcctgcgcat cagggcgcgg cggcctacat
      421 cgacggcacc gagcgcacct tcctcgagag gtacagcgat tacttctggg ccgccatcct
      481 gcttctctcg ggactcgggt ctgccggcgc ctggctgcgg caatattcga agcgggatga
      541 aaaagaacaa agcacgctgc atcgcgacaa gctgctcgcc atgatttcga aagtgcgcca
      601 ggcggaatca tcggaagaat tgaccgcgat gcaatgcgag gtggacggcg tcttgcggga
      661 agcgctggag agctacgacg acggcgcgat cgaggaaggc gacctgtcgg ccatcggtct
      721 cgtgctggaa cagttccacc atgccgttgc cgaccgacgc atcgcgatgg tgggtgtcag
      781 cacgcccgat ctggcgcgcc ggcgcgcgct ttaggcgggg atcgctaccc ccgcgaagcg
      841 cggttcctgc agcgcgttgc tgggccacag gacgaaattt caagacacag aaatggcgct
      901 ggatcggcgc aatgtttttt gtttcctgac atgacaaaac cgtaacgtgc acctgccctg
      961 gcgggatggc gcccgcggaa cccgccttgt agacttgcgc ctcaatcagt ccggggggac
     1021 gatgcgtctg aagacgatcc tgcttggggt cgcggtcgtc gcggtctcgt ttttcgtcag
     1081 cctgaaggcc atggactggc tgtcgccacg cagcacggtc cctgcgccgg tgcttgtcga
     1141 attgccgccg ctgccccccg cgccgcgcag ttccagcatc gtcgcaccga tcgtgattac
     1201 gctcaccacc attcgcgacg ccgccgatcg tggcgcgccg cgcaccttcg ccggcaaggc
     1261 cgataacccg gttgcgcaga tcctgcagaa taccgacatc ggctggaccg catcgcgcgg
     1321 accgatatcc gcgaccggcg cgcaggacgt gctgtcgctg gcaaccccgt tgacgggaac
     1381 gctgaatgtg accggctcgc tgtcggcgaa gacgacgggc gcgatcggcg acgcgctcgg
     1441 cagtctgctc ggcggcgatg ccgccaagcg gatcggcagc gtgaacatca agtcgctcaa
     1501 tgcccatgcc gagatcaagg gcaacgtcac catcacagcg cggccaaaac tcgccgcggc
     1561 ctggcggatc gagccgaatt tgaccgcgca ggtgaccctg agcgacacca acctttcggt
     1621 ttcgggcgcg cgcgtcaatg tcccggcgca ggtcaagccg ctgatcgaca agaccgtcgc
     1681 cgatcagctc gacgcgctgc aggcgcggat tcgcaacgat cccgcattcg agaacaacgc
     1741 caagctgcaa tgggccaagg cctgccgctc gattccgctg caaggcccgg gcacggcctc
     1801 gtcgctgccg gcactctggc tggagctgcg gccgacccgc gcgatcgccg cgcaaccgcg
     1861 catcgatgca gcgacggtga cgctgatgct cggcatcgag gcggagacaa ggatcactcc
     1921 ggcgcagacc aagcccaact gtccgtttcc ggcgacgatc gacatcgtcc cgccgggccc
     1981 gagccggatc accatcggcg tgccgatcga tctgccgttt accgacatca acaagatcgt
     2041 cgaggcgcag ttcaccggcc ggagctttcc cgaagatgga tcgggctcgg tcgacgtcac
     2101 cgtgaagcgc gccagcgtcg cggcatcggg cgaccggctt ctgatctcgc ttctggtcaa
     2161 ggccaaggaa aaaaagagtt tcgttggact gggtggcgag gccaatattc atatctgggg
     2221 caagccggtg ctcgaccaag aacagcagac cttgcggctg accgacatcg aactcgccgt
     2281 cgaatcagag gcggcgctcg gcctgctcgg ggctgcggcg cgcgccgccg tgccgcatct
     2341 gcaaaaggtg ctggcggaca aggccaccat cgatctcaag ccattcgcct cgaatgcgca
     2401 aaacaagatc gccgcgctga tatccgattt tcagaagaac gaggacggcg tgcggatcgc
     2461 ggcggaaatc accagcctgc gccttgccga catcgccttc gattccaaaa ccctgcgcgt
     2521 gatcgccgaa gccgccggcg cgatcaacgt ctccatcacc gcgctgccgg ggctgtgagc
     2581 tctgatcgcc ctctcccgcg ttgcgtcgcg ttgcgggaga gggcggcagg catcgcgcac
     2641 gcatggcgag ccttactgac aaccctgccg tttgctgcta agcaagagtg gtcgtggcga
     2701 tcggaggctg acatgcagcg tttgtcactc ttgcttgcgg ccattggtct ttgtgtggtc
     2761 ctgctcgtct ccggatgtgg cgacaacaag caaccggcgg cttccgcgcc gccgccagcc
     2821 gtgaccgtcg tcaaggtcac cgccacggag atcaaaccaa cgacgacctt cacgggccgg
     2881 atcgaagcca agaacaaggt cgatctgcgt gcacgcgtcg acggcttcct cgagaagcga
     2941 ttgtttgcgg aaggcgcgga cgtcgcggaa agcgaccttc tgttcgttat cgaaaaggga
     3001 ctctatcagg ccgcggtcga tcaggcgaag gctgcgctcg aaacggcgga atcaaccctc
     3061 aagctcgccg atctcgacgt cgaccgccag acccaactgt ttcagcgcaa cgtcacggcc
     3121 caggcgacac tggaacaggc caccgccaag cagggcgagg ctcgcggcaa catgctggcg
     3181 cagaaagcgg cgctggaaaa agccgagctt cagctgagct acaccgatat caagtcaccg
     3241 atccccggac gcatcggtcg agcgtcggtg ccggtcggca acttcgtcag cccgtcgaat
     3301 ggcgcgctgg ccacggtcgt cagtcaggac cctatctacg ccagcttccc ggtcacccag
     3361 cgcgagatgc tcgccgtgcg caagcagcaa gccgagggca aaggcagcga gtatgtcatc
     3421 tatgtccagc tcgcggacgg cagccgctat cccggcgccg gaaaaatcga cttcctcgac
     3481 aatacggtga atcaggggac cgacacggtc caggtgcggg cggcgttcgc caatcccgac
     3541 cgcgtcctgg tcgacggtca actggtgacg gtcgtcgccg aagagggcaa gggtgagagc
     3601 gcgctcgtgg tgccgcagca agcattgcag gtcgatcaaa ccggtcctta cgtcctcgtc
     3661 gtcgacaagg acaacaagat tcaaatccgg cgcgtcgaga ctgatgttgc gcgcgggtga
     3721 acattgtcat ccgcaagggg ctcgcggcga atgagctcat cgttaccgaa ggcattcagc
     3781 gggtgcgtcc agggcaggtt gttgtgccga ccgaagccag gccgggagcg tagaacatgt
     3841 tgtcgagcat cttcatcgat cgaccccgat tagcggtcgt catctcgatc gtcatcaccg
     3901 ttgccggcct cgtcgcgctc tttcaaattc cgatcgcgca atttcccaat atcgttccgc
     3961 cgcagatcgc cgtcaccgca tcctatgccg gcgccagcgc ggaggtggtc gaggccaccg
     4021 ttgcccaacc gatcgaggca cgcgtcatcg gcgtcgacaa tatgctctac atgaagtcga
     4081 ccagcggcaa tgacggcagc tatacgctca acatcacctt cgcggtgggg acggatcctg
     4141 acctcaatac cgtcaaggtg cagaaccgcg tcggtctcgc cgaggcccag ctgcccgccg
     4201 aagtgcgcgc gcagggcatc agcgtcacca agaaatcctc cgccctgctg caggttatcg
     4261 cgctgacctc tcccgacggc cgctatgacc agctgttcct gagcaattac gccaccatca
     4321 acgtcattga ccagctgaag cgggtacccg gcgtgggcga cgtctcgctg ttttcaccat
     4381 ccgattacag catgaaggtg tggctcaaca ctgatcggct taccagtttc ggcctcgttc
     4441 ccggcgatat cgccaatgcc ttgaaggccc agaacatcca ggccgcggtc ggacgcatcg
     4501 gcgcgcaacc cgcactgccc gaccagcaat ttcaactgaa catccagtcc aaaggccgcc
     4561 tcgcaaacgt cgaggaattc ggcgcggttg tggtgcgcgc caatcccgac ggctcgttgg
     4621 tgcgggtccg cgacgttgcc cgcgtcgagc tcgcggcgcg cttgtcggaa tcgctcggcc
     4681 ggctggatgg gcagccggcg gcggtcatcg ggatttacca gtcgccgggg ggcaacgcgc
     4741 tcgatagcgc gcaacggatc ggcaagattc tccaggaact caaaacctcg ttcccggagg
     4801 gggtcgatta caggatcacc tacgacacca cgatcttcgt caaggaaagc atcaagggtg
     4861 tgctgcatac gctggtcgag gcctttgtcc ttgttgtcat cgtcgtgttc ctgttccttg
     4921 gcagcgtgcg ggccacgctg atcccgttgc tggcagttcc cgtggcgctg atcggcactt
     4981 tcgccgtgat gctcgcgctc ggatattccg ccaacaccgt ctcgctgctt gcgctggtgc
     5041 tggccatcgg catcgtggtc gacgatgcga tcgtggtggt ggaagccgcc gaggcgcagc
     5101 ttgaaaagga cccttcgctg tcgccggccg aggcgtcgcg gcgggcgatg gccgagatca
     5161 ccgcgccgat catcgccatc acgcttgtcc tgctttcggt gttcgttccg gtcgccttca
     5221 tacccggtat ttccggcgaa ctgttccgtc agttcgcggt ggcggtctcg gtgtcgatgg
     5281 tgatctcggc gatcaatgcg ctgacgctgt cgccggccct gtgcgcggtt ttcctgtcgg
     5341 ccagccacgg accccgacgc ggcctgatcg gttacgtgct gcgcggaatc gacaaggcgc
     5401 gtgatgggta ttcactggtc gtccacaaga ccgtccgctt cgcggtttac agcctgctgg
     5461 tccttgtggc ggtcatcgcc ggcgccggct ggctgttcaa ggtcacgccg accggcttcc
     5521 tgccttccga ggaccagggc gcggtgttcg gcgagattca gttgccggag ggcgcctcgg
     5581 tcaaccgcac cgccgcggtt accaaacgcg tcgaggaaat cgcccgcaat acccccggca
     5641 ttgccagcgt gacgtcggtc atcggctaca gcctgatcga tggccttacc aaatcgaaca
     5701 gcgcgctgct gatcctcgcg ctcaatccgt tcgatgagcg caagaccgcg ccggtgtcgg
     5761 cgaatgggat cattgcaggc ttgatgggcc agtttcaggg gatcccggag gccatcgtct
     5821 tcgcctataa tctgccgccc atcatcggat tgggcaccgg cagcggcttc gaattccagc
     5881 ttcagagtca atccggcgcg acccctgccg agattgccgc ggtagcccgc ggcatggtgt
     5941 ttgccgcaaa ccaggacgcg acgctgcgcc gcgtctttac gacctacagc gccaataccc
     6001 cgcaactcta tctcgacatc gatcgggaaa aagttcagac actcggcgtc aacgtggccg
     6061 atgtcttcaa tgcgctgcag tcggtgcttg gcagtgcgta catcaacgat ttcaatctgt
     6121 atgggcgcac ctggcaggtc aacattcaag gcgaagcctc ggagcgcgca cgcgttgacg
     6181 atatctatcg catcaacgtc cgcaacagga atggcgatat ggtttcgatt cgtgccttcg
     6241 cggcggcacg tttgatcctg gggcctcact cgatcatccg ctacaacaac ttccgcagcg
     6301 tgacattgaa tggcgggccg gcgccgggct attcctccgg cgatgcgatc gccgcgatgg
     6361 agaagatatc ggctgcaacg ctgccgcgcg gctacggtta cgaatggacc ggaaccgcat
     6421 tgcaggagaa ggaagcctcg ggcaagacca cgacaatcct ggcgctggcc gtgctgttcg
     6481 catacctgtt tctggttggt ctctacgaga gctggtcgat ccctgttccc gtgctgttgt
     6541 cgatttcggt cggcgtgctc ggttcgatgg ccggccttct ggcgttcggg ctcgacaaca
     6601 atctttacgc ccagatcggc ctcgtggtgc tgatcgcgct ggcggcgaag aacggcatcc
     6661 tgatcgtcga attcgccaag gagcggcgcg agcagggcct gccgatcgag caggctgctg
     6721 tcgaaggcgc ccgggagcgg ttccgcgccg tcatgatgac gagctttgcc ttcatcgcgg
     6781 gcctggtgcc gctcgtcatc gcgacgggcg ccggacagtt gagccggcgc ggcgtcggca
     6841 ccgcggtatt cagcggaatg atcgcggcat cgttcctcgg ggttttcctg atcccgttgc
     6901 tctacgccgt ctttcagcac ttgcgggaaa gaatcaagcg gcagccggca aagcgagatt
     6961 tgcaagccga cgccgtcgcc aagcctgacc attagattca acaccaatgg cgacgccgaa
     7021 atgcggccgc cggaggttcg cttactccga cgggtaacga atgtgggagg caaatgggag
     7081 accttaaatg tgcgtgcgag atatgtcgtc ttgaaaatcg gctcagcaat ggcaaaaccg
     7141 aaatgatctc atgatgaggc ggcaagcacc gtccggtatc tgcctaaacg aaggtaattg
     7201 ttgccagaca aagaagatcg ctcccaatat ctggattgcg agcacgaaca gatagatggc
     7261 tgctttggga tggtcgccgt tcatcttgat cgagcggatg gtatcagttc aatcagtacc
     7321 gaatcaaaca gaccccttct ccgagcgggt ttgactcggt cacgatgtct tcggccacag
     7381 accctcaaat ctgttcagct ctgaggcccc atcagccgct gagcacgctc tgcgcagagt
     7441 atgtgatggt ggaggttggc gatccattgc cggccggaag ccgtcatgtt gagatatttt
     7501 atcccgtcgt cgaggtgcat cgagacgctg tcccagaaga acgccgggta cctctcaaca
     7561 atgtcggcgg gcgacgaata gccaagttgc ctgttggtgc cgatctcctc gaactcccaa
     7621 tagagcgcgt ttgctttgcg cgaatacagg ggatcgccga gctgcccgat gaaatcggaa
     7681 gcctgtacca gtttcggctc gagaccgctc gcctccgcgc gatcttccgg gactggaaag
     7741 cgtgtcatct cgattgccgc cgcaacgcgc gcagggtcga gaattggaga attctcgaat
     7801 cgctcgaata caaatagttt tgaccggtcg acgtgatacg gcgcgagcgc cgcatccgag
     7861 gcgccgcgcg ggagcacgat tttcgtcccg tcgggatcaa tgatgaattc agtttcggta
     7921 tcgccgctca ggacgccacg cacatagccg atatcatgaa aaagacaggc ggcaatcagg
     7981 tggccatagt catcaggctc tattcgttca tgcaccatca tgccatgcag catgtcgcgt
     8041 ccgaccatgg tgacctgcag cgtgtgttcg taattgtggt aaagcgcgtc gctcctggcc
     8101 agacattcga tggtgctccg cgccgcagag ccgatgcgct ccgccatatc gtcgcatgtc
     8161 gaaccgaaaa tttggcgaaa atccctggtc aggaaattgc cgagcttgtt tgccgcgatg
     8221 gccgttatcg ttttcatcgg cgttctccga atgacccgcg atcgtttcga agtccatgca
     8281 gtgtagatgc ccatgccagt agtcagctat cggagccggc gccgaccgca gtggcagaca
     8341 ttcatgttat tcgcggcggc cttctcaaat gcagttcgtg tatttcgcag cattcccccg
     8401 tcagtccatt gcgacggggg aatggcattt tcccgcgcac cggctcgcct aatcctcgaa
     8461 tttgaacgaa acgttgagct tggcacggta ggcttcgatc ttgcccttgg tatccagttg
     8521 tagatcgagt ttcacgactt ccgcgacacg aagttcgcgc agggattttc cggcccggtc
     8581 gaccgcggcc tgggccgcct tctcccagga atccttgctg gtaccgatca gttcaatgac
     8641 tttgtagacg ctctccgtca tgtcgtcctc ccgttcggta tttggcagga cgcagggtgc
     8701 tcctgccgcc aacaaggtta acatcgggac cacggcttcg aaaggcgcaa tccagagacg
     8761 gtggcgggct gagcctgccc aaaaaccggc tttgccggcg tgggcgaagg cggctaaaag
     8821 acagcctcaa accaagccct taacaagaac gatcaggcag gaagaaacat gaagtcaatt
     8881 gcggccgcgg ttgcggccag tttcgttttg gcggggtccg gcactgcggc gaatgcgcag
     8941 atctccgacg acgtcgtcaa gatcggcgcc ctcaccgata tgtccagcct ttatgccgac
     9001 gcgaccggca agggctcgct ggccgccgtc cagatggcgg ttgccgacta cggcgccaag
     9061 gtgaagggca aaccggtcga ggtggtttcc gctgaccatc agaacaagcc cgacatcggc
     9121 gttggcatcg cgcgcaactg gtatgacaac gagaaggtcg acgcgatctt cgacgtgccg
     9181 acctcgtcgg tcgcgctgcc agttt
//
LOCUS       DATNOI010000004         3056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_893539,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000004 DATNOI010000000
VERSION     DATNOI010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893539"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VN968_00070"
     CDS             <1..716
                     /locus_tag="VN968_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXL27678.1"
                     /translation="AIYKVDGVRTLNESYYSLVRRGLMGLDYVFRRRGPLTMAPSQLG
                     IFTRSDPHRERANIQFHVQPLSLDKFGDPLHRFPAITVSACNLQPTSRGTVRLRSARP
                     DEAPSIAPNYLTTSEDREVAADAIRTTRRLMKQPALAPYRPREFLPGPSVGDDDAALA
                     KAAGDIGTTIFHPVGTAKMGTASDPTAVVDERLRFIGLAGLRVVDASVMPTITSGNTN
                     TPTAMIAEKGAAMILEDAR"
     gene            complement(813..1022)
                     /locus_tag="VN968_00075"
     CDS             complement(813..1022)
                     /locus_tag="VN968_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27679.1"
                     /translation="MADHAGVEHHHKAAEHHEQAAKHHREAARHHEAGDHEKAAHHAH
                     SAHGHASHAEEHHAEASRHHAEHHG"
     gene            1261..2211
                     /locus_tag="VN968_00080"
     CDS             1261..2211
                     /locus_tag="VN968_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXL27680.1"
                     /translation="MSGFDHDHDNEGPGVSRRRVLECMTWAGTGVLWTISGGVPRSLG
                     LVGSAQAAESAGMTFLQISDSHVGFDKPANPNALGTLEEAIAKINAMPAKPSFMIHTG
                     DISHLSKAAEFDNADRIISQAKLDVHYVPGEHDFLDEENKLYKERYGRGAKGAGWYSF
                     DADGVHFIGLVNVVNLKAGGLGNLGSEQLEWLEDDLKGKSKSTPIVVFAHIPLWTVYP
                     EWGWGTEDGARALDYLKGFGSVTVLNGHIHQVMQKVEGNVTFHTARSTAFPQPAPGTA
                     PSPGPMKVADDKLRGLLGVASINFKRGTQNLAIIDTPLQG"
     gene            2224..2559
                     /locus_tag="VN968_00085"
     CDS             2224..2559
                     /locus_tag="VN968_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HXL27681.1"
                     /translation="MTSVTPRSHGFRLAMAAAISLCLGAAHAEETTTNVMIDNFTFQP
                     AELTVKVGTTVTWKNRDDIPHTVVSAGKFRSKTLDTDDSYTFTFTAAGDYKYFCSLHP
                     HMTGTIKVE"
     gene            2651..>3056
                     /locus_tag="VN968_00090"
     CDS             2651..>3056
                     /locus_tag="VN968_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXL27682.1"
                     /translation="MPVNAANDDPEKARRFRDAALPYLDDVYTLARYLLRDSADAEDA
                     VQECYLRALKHFDSYRGPAMKPWLFAILRNVCRAEFARRASSPTAPIEDVPEDAAQTP
                     LWHDAPETPEAQMLHRWDASTIRRLVEALAEPF"
BASE COUNT          576 a          991 c          928 g          561 t
ORIGIN      
        1 gcgcgatcta caaggtcgat ggcgttcgca cgctcaacga gagctattat tcgctggtga
       61 ggcgcggcct gatgggcctc gattatgttt tccgccgccg tggcccgctg accatggcac
      121 cttcgcagct cggcattttc acccgttcgg atccgcatcg cgaacgcgcc aacatccagt
      181 tccacgtgca gccgctgtcg ctcgacaagt tcggcgatcc cctgcaccgc ttccccgcca
      241 tcacggtgag cgcctgcaat ctgcagccga cctcgcgcgg cactgtgcgc ctgcgttccg
      301 ccaggcccga cgaggcgccg tcgatcgcgc cgaactatct cacgaccagc gaggaccgcg
      361 aggtcgccgc cgacgccatc cgcaccaccc gccgcctgat gaaacaaccc gcgctcgcgc
      421 cctatcgccc gcgggaattc ttgccgggcc cgtcggtcgg tgacgacgat gcggcgctgg
      481 cgaaggccgc gggcgatatc ggcaccacga tcttccatcc cgttggaacc gccaagatgg
      541 gtacggccag cgatccgacg gcggtggtcg atgaacgctt gcgatttatt ggcctcgccg
      601 ggcttcgcgt ggtcgatgcc tcggtgatgc cgacgatcac ttcggggaat accaacaccc
      661 ccaccgcgat gatcgccgag aaaggcgccg cgatgatcct tgaggatgcg cggtagaggt
      721 tcaaaaaaac gccgtccctc ggaaagggac ggccaaacgc aaacgcaacg cttatgactt
      781 cttcttattg ttgtgttggc cgtaacctgg tcttaaccgt gatgttccgc gtgatggcgg
      841 ctggcctcgg catgatgttc ctcggcatgg ctggcatgcc cgtgggcgct atgggcgtgg
      901 tgcgccgcct tctcgtgatc gccggcctca tggtgcctgg cggcttcgcg gtggtgcttt
      961 gctgcttgct cgtggtgttc ggcggccttg tgatggtgct cgacgcctgc gtggtcagcc
     1021 atatttctct cccggttcag tgcgaagggt ctgcaagatc tacgcaagtc gcctgacact
     1081 atgctgacag ccgcacgaca ggtttgttgc gggcggccta tcgctgaggc cgggcacatc
     1141 ggccgtcccg atcacgaaaa cgtcatcgcg tgaccaccag ttgcctgaaa ttccgggaat
     1201 aaaccccgcc ctcgcccgat ccagtcccca caacccaatc aagggtccat ggagattggt
     1261 atgagcggat tcgatcacga ccacgataac gaaggcccgg gcgtcagccg ccggcgggtg
     1321 ctggaatgca tgacctgggc cggcaccggc gtgctctgga cgatttcggg cggtgtgccc
     1381 cgttcactgg gccttgtcgg ttccgcgcag gccgccgaat cggccggcat gacgttcctg
     1441 cagatcagcg acagccatgt cggtttcgac aagccggcca atcccaacgc gctcggcacg
     1501 ctggaggagg ccatcgccaa gatcaacgcg atgccggcga aaccgtcgtt catgattcac
     1561 accggcgata tcagccatct gtccaaggcg gccgaattcg acaacgccga ccgtatcatc
     1621 tcgcaggcca agctcgatgt gcattacgtt cccggcgagc acgatttcct cgacgaagag
     1681 aacaagctct ataaggagcg ttatggacgc ggcgccaagg gcgccggctg gtactcgttc
     1741 gacgccgacg gcgtccactt catcggtctc gtcaacgtcg tcaatctgaa ggccggcggc
     1801 ctcggcaatc tcggctccga gcagcttgaa tggctcgagg atgatctcaa ggggaaatcg
     1861 aaatccacgc cgatcgtcgt attcgcccat attccgctgt ggacggtcta tccggaatgg
     1921 ggttggggaa ccgaggatgg cgcccgtgcc ctggattacc tcaagggctt cggctcggta
     1981 accgtattga acggccacat ccaccaggtg atgcagaagg tcgagggcaa cgtcaccttc
     2041 cacaccgcgc gctcgaccgc gttcccgcag ccggcgccgg gcaccgcccc ttcgcccgga
     2101 ccgatgaagg tcgccgacga caagcttcgc ggcctgctcg gcgtcgccag catcaacttc
     2161 aagcgcggca ctcaaaacct cgcgatcatc gacacgccac tgcagggcta agggaaattc
     2221 ataatgacgt cagtaactcc gcgcagccat ggttttcgcc tggccatggc tgcggcaata
     2281 tccttgtgcc tcggcgccgc tcatgccgag gagacgacga caaacgtgat gatcgacaat
     2341 ttcacctttc agccggcaga gctcacggtg aaggtcggca ccaccgtgac ctggaaaaat
     2401 cgcgatgaca tcccccacac ggtggtgtca gcgggcaagt tcagatcgaa gacgctggat
     2461 accgacgaca gctatacgtt cacgttcacg gcggcgggcg attacaagta cttttgttcg
     2521 ctgcacccgc acatgacggg gacgatcaag gttgagtagc ggtcaaaatc aaggcatcac
     2581 agccatgccc ggccggtgga ttgccgccgg ccgggcaccc gtcatttcta ccaacgccgg
     2641 gcaaagcgcg atgccggtca acgcagcgaa cgacgatccc gaaaaggcac ggcggtttcg
     2701 cgacgccgcg ctgccgtatc ttgacgatgt ctatacgctg gcgcgctacc tgcttcgcga
     2761 ctccgccgac gccgaggacg cggtgcagga atgctatctg cgggcgttga agcatttcga
     2821 cagctatcgc gggccggcaa tgaagccgtg gctgttcgcc atcctgcgta acgtctgtcg
     2881 cgccgaattc gcgcggcgcg caagttcgcc gaccgccccg atcgaggatg taccggaaga
     2941 cgcggcgcaa acgccgctct ggcacgacgc gccggaaacg ccggaggcac agatgttaca
     3001 ccgatgggac gcctcgacga tccgcaggct tgtggaggcg ctggccgaac cgttcc
//
LOCUS       DATNOI010000005        15121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1785119,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000005 DATNOI010000000
VERSION     DATNOI010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15121
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785119"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VN968_00095"
     CDS             complement(<1..242)
                     /locus_tag="VN968_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrB/QacA family drug resistance transporter"
                     /protein_id="HXL27683.1"
                     /translation="MTALQSTIDPPASADVSGDRAQTTSAPDQAEPAVSSKIWLAVAG
                     ATLGAFMAVLNIQIVNASLADIQGAIGAGIDDGGWIS"
     gene            complement(363..1523)
                     /locus_tag="VN968_00100"
     CDS             complement(363..1523)
                     /locus_tag="VN968_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HXL27684.1"
                     /translation="MTTASYVSDSNTKISLRPSRQAIKRAALALALTLGVAGAADFGY
                     GYLTTGRYLEATDDAYVKADSTIIAPKVSGYIAEVLVGDNQSVKAGQLLARIDNRDFE
                     AALAQAHADVDASGAAVRNLDAQIALQQPIIEQESADVAAAEANLKFAQEEQARYDGL
                     MKTGSGTIQRAQQTDAALREKTAQLQHGKSGLLAAQKKVDVLTTERAKAAAQVDRARA
                     VERQMALNLSYTKIIAPVDGTVGARSLRVGQFVQAGTQLMAVVPLDAVYVVANFKETQ
                     LTHVRNGQPVEVRIDGFHSTKLKGHVDSLSPASGLEFALLPPDNATGNFTKIVQRVPV
                     KIVLDDHNLTGLLRPGMSAEPTVNTKAAVLAEHDQQTSATRLASNRVKQPGS"
     gene            1660..2571
                     /locus_tag="VN968_00105"
     CDS             1660..2571
                     /locus_tag="VN968_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL27685.1"
                     /translation="MDRLTSLTAFVRVVDSGGFSAAGRRLNMSTTMVSNHVQALEDRL
                     GARLLHRTTRKVSLTEVGKAYYDRCIQILADLEQADDIAGALQSTPRGTLRVYTATHI
                     VPFIAPVVAGFLASYPDVKVDLTMGERTIDMIDEGYDVAIRLTPPPDSSLIVRSLATW
                     RHVLCCSPGYIDKHGRPQQLSELANHNCVRHALYPYEDEWRFVDRKDTPTSVRVSGNL
                     FSNSGDTLRTAALQGLGVFLAPGFLIADDLEAGRLVRLLPEYRPVEFAMNAVYPHRHH
                     LSAKVRTFIDLLALHSAEHQKLIIPYA"
     gene            complement(2664..2837)
                     /gene="ccoS"
                     /locus_tag="VN968_00110"
     CDS             complement(2664..2837)
                     /gene="ccoS"
                     /locus_tag="VN968_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HXL27686.1"
                     /translation="MIDFLYLVPIALALGLTGLAAFLWSLRSGQYEDLDGAAERILFQ
                     DDDAPIEDHKAGR"
     gene            complement(2834..5119)
                     /locus_tag="VN968_00115"
     CDS             complement(2834..5119)
                     /locus_tag="VN968_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HXL27687.1"
                     /translation="MSCCAAGAEAFIARTDPAVARQEVLLASHRISEDLLQTDLSVPG
                     IHCGGCIQAIERTLGALPDIEHARVNLSTKRVTVRWRGGDPPPFIEALDRIGYKAHLF
                     DVDGDRKDAVQGELVRALAVAGFAASNIMLLSVSVWSGAEPATRDMFHWLSALIALPA
                     LAYSGRVFFRSAWQSLRHGRTNMDVPISVGILLATGMSLYETFQGGRHAYFDAAISLV
                     FFLLIGRTLDHMMRERARSAVKGLARLAARGALVLQPDGARVYLPVNEIAPGMNILLA
                     AGERVPVDGRVTSGRSELDVSLVSGESLPQPVVEGALLQAGTMNLTGPLTIVATAAAK
                     DSFLAEMTRMMEVAEGGRSNYRRIADRAASLYAPVVHLTALLTLIGWMAAAGDLHQAV
                     TIAIAVLIITCPCALGLAVPMVQVVAAARLFENGIMVKDGSALERLAEIDTVVFDKTG
                     TLTLGNPQLVNADAIPPEVLTLAGAIASHSRHPYSRALAVAGNDQVHAAFTAFTDVSE
                     LPGSGLEVRAGTTIYRLGRREWALSGDRALPVSARSEVVLSRDGAYLAGFDLHDSLRR
                     DAREAIAALKDEGLPVEVLSGDREQPVSELATALGLDYRANVSPREKVARIKALCSAG
                     RKVLMAGDGLNDAPALTSAHASMAPASAADIGRNAADLVFLHESLFAVPQAIAVAREA
                     KHLVRQNLALAIGYNAIAIPVAVCGYVTPLIAAVAMSVSSIAVIANALRINRRRAGLQ
                     QDKAIDAGQRIASHIAFGSHR"
     gene            complement(5168..5953)
                     /locus_tag="VN968_00120"
     CDS             complement(5168..5953)
                     /locus_tag="VN968_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HXL27688.1"
                     /translation="MTMIPSVVPPLPTENKWSRSLKPADAFEWLSDGWKDFWIQPVTS
                     LGYGLIVFLISIAIVAGLYALGWDYILFPALAGFMVIGPILAVGLYEKSRRIAAGEPT
                     SWNDILFVHPKSGGQILFTGVLLCLLMLVWMRAAVLIYALFFGLLPFPGLDQIVPMLF
                     KTEVGWAMLIVGGLVGGLFASFSFAISAFSIPMLLNEKTDALTAMGSSMALVWNNLPA
                     MLTWGAIVLALFLFSLATGLVGLIVVFPLLGHGTWHAYQAVAR"
     gene            complement(5973..6164)
                     /locus_tag="VN968_00125"
     CDS             complement(5973..6164)
                     /locus_tag="VN968_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27689.1"
                     /translation="MAVSLSVSAGVLLVVLANVHLVYVATISQPDCVAHVRQGEGGLP
                     HRPFSAAQSSCSPRSIAQK"
     gene            complement(6203..7081)
                     /gene="ccoP"
                     /locus_tag="VN968_00130"
     CDS             complement(6203..7081)
                     /gene="ccoP"
                     /locus_tag="VN968_00130"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HXL27690.1"
                     /translation="MALDERDTYTGHMLTGHEWNGIKELNTPVPRLIYFFFSLAFLFS
                     VGYWVLMPAWPLGTTFTKGLLHTDQRQVVEASLEQATLARDVWTKKIEGDSFAAVQSD
                     PRLMAVVRQSGRTLFGDNCAVCHGLDAKGGKGFPNLTTTSWLWGGTPEAVFETIRVGI
                     NSTHPKTRVSQMPAFGRDQMIPRADVLKVATFVHSLSSPNSGTADPKVVEAGKAIFAA
                     NCVSCHGADAKGNLELGAPNLTDKLWIYGGNLETIDTSVWGGRQGHMPTWEGRLSALD
                     RKILALYLVDKRAPDQ"
     gene            complement(7072..7242)
                     /locus_tag="VN968_00135"
     CDS             complement(7072..7242)
                     /locus_tag="VN968_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HXL27691.1"
                     /translation="MNVAHDTLAWFSKSFGLFYLIALSVIVLVYVYWPSNKKQFDRAA
                     KSILSDEDRPWR"
     gene            complement(7245..7976)
                     /gene="ccoO"
                     /locus_tag="VN968_00140"
     CDS             complement(7245..7976)
                     /gene="ccoO"
                     /locus_tag="VN968_00140"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HXL27692.1"
                     /translation="MFGFHYRLERKAIGLALGIVGVASIGGLVEIAPLFTIHQTVEDV
                     PDMRLYTPLELAGRNIYIREGCYACHSQMIRTLRDEVERYGPYSLAVESKYDHPMLWG
                     SKRTGPDLARIGEKYSDQWHAAHLNNPRDVVSVSVMPAYTWLLRTSLRTDDLGLHLKA
                     QRAVGVPYTDDMIANAATDAYGQASPDSPWAAGVVKRYGKATNMRAFDDRPNELTEMD
                     AVVAYLQVLGHLTDAAYKPVASTEK"
     gene            complement(7980..9632)
                     /gene="ccoN"
                     /locus_tag="VN968_00145"
     CDS             complement(7980..9632)
                     /gene="ccoN"
                     /locus_tag="VN968_00145"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HXL27693.1"
                     /translation="MIAQLTRSERQLALVMLVITAVFGLVVAIVGRNDPLGIHGALVA
                     LLAIVATFRVITDYYQPEPNEDRSRHYYDDPTKVGIVLAMAWAVFGMFVGDWVAWLLA
                     YPDLTFDAAWSSFGRLRPVHTTGVIFGFGGNGLIATSFYVMQRTSRARLAGQLTPWFV
                     LFGYNLFCIIAVTGYMMGVTQSKEYAEPEWYADVLLVIVWVTYLVLYLQTLARRKEPH
                     IYVANWYYLAFILVVAMLHIVNNLAIPVSLGHSKSYSAFSGVQDAMTQWWYGHNAVAF
                     FLTAGFLGMMYYFLPKQAGRPLFSYRLSIISFWGITFLYMWAGSHHLHYTALPQWVQT
                     LGMTFSVMLLIPSWASAANALLTLNGAWDKVRDDATLRFMMVAAVFYGLTTFEGSFMA
                     IRPVNSLSHYTDWTVGHVHAGALGWVAMITFGSIYASVPWLWKCKTMYSPRLVEAHFW
                     FAVAGTIIYVFAMWNSGIVQGLMWRTYSESGTLTYSFIDSLIAMHPYYIARAVGGLLF
                     LIGAVIGAYNIWMTIRMVSQTADELADRPELLPAGAALQQAE"
     gene            10384..11379
                     /locus_tag="VN968_00150"
     CDS             10384..11379
                     /locus_tag="VN968_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HXL27694.1"
                     /translation="MNLNTIVEVLIRKAGQFGFPAGMVLVLAIHSPPATFAQDGIDGV
                     TGFARDPVHVAAWPAGRKVAVSFALFVEEFGFGQGPVFRPDLASRNPDLVNEAFRQYA
                     IDWGIPRVGRLFRELDVPLSIVLNAEFPATHASVWKEFRATQPNAPVVAHGMNNSSHM
                     LPLGRGLAEQKAYIRRTLELIAATTGVKPTGWSSPSVYSNGDTMQAMAAEGIAYNLDQ
                     MDSDIISRLKTPDGSLVLLPYPVVTVDMGQNLARMKTPAEIEALWLDYVSELAREARA
                     NPAREATTVVIGIHPFVIGTPDGAAALRRVLLRLKSDDAVWLTDTDAILKAAALK"
     gene            11458..11643
                     /locus_tag="VN968_00155"
     CDS             11458..11643
                     /locus_tag="VN968_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5993 family protein"
                     /protein_id="HXL27695.1"
                     /translation="MSSLQSGKKMEFTVLFLCVLVVMAVSWRGSHRLALALFSIVLVA
                     SVATYLHHATDTLNLSF"
     gene            11680..12231
                     /locus_tag="VN968_00160"
     CDS             11680..12231
                     /locus_tag="VN968_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HXL27696.1"
                     /translation="MKRSIGVTLNALGLYGIALVLLAAFAGQILLGELPCPLCLLQRV
                     LFAMLAIGPILNIRFGPHPGHYALSLLAAIVGATVSARQILLHIMPRDPGYGSALLGY
                     HYYTWAFIAFAAAIALIAIVLLFDDRSPDNKVAGPVGLFASAAVWLVISLTAINAAST
                     LLECGFKACPDNPAFYELLKRSP"
     gene            complement(12336..12953)
                     /locus_tag="VN968_00165"
     CDS             complement(12336..12953)
                     /locus_tag="VN968_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Isoquinoline 1-oxidoreductase subunit"
                     /protein_id="HXL27697.1"
                     /translation="MKSEMRVRILIAVAALTTSVLTSYAASQTSANTLASPESFGAIN
                     APEARSAAMFTELGKVLTHPRCVNCHPAGDRPHQANEGRLHQPPVERGADGRGLPAMR
                     CSICHQNANFDPGRVPGHPDWHLAPREMAWEGKAVGEICAQIKDPARNGGRKPEDLIH
                     HIGEDTLVGWAWAPGYGRSPAPGTQKEAGALVEAWVKSGAICPGN"
     gene            complement(12993..13508)
                     /locus_tag="VN968_00170"
     CDS             complement(12993..13508)
                     /locus_tag="VN968_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXL27698.1"
                     /translation="MTTLAITINGRAYGPTEVRDDLTMNDFLREYLGMTGTKFGCGAA
                     QCLSCAVIVDRPDGTSYTSPTCIVPAASFDGKAIRTVEGHARNGELSDLQKAFIDHFA
                     FQCGYCTAGFLNEGQVLLERLAKTPVARAKLETTIAEALDGHICRCTGYIKYHEAVRD
                     VILADPKSYLL"
     gene            complement(13505..>15121)
                     /locus_tag="VN968_00175"
     CDS             complement(13505..>15121)
                     /locus_tag="VN968_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL27699.1"
                     /translation="DRATGKISGFAADHVLDGGGLANFSANVATVGAAAAIGIYDIPK
                     VDVTTVALHSRGVTAGSMRGYGTLQPMTALEVLIDEAAAALPLDPIEFRRRNALKTGG
                     KTMTGNPYIVSIRTPEILDKLEKHPIWQQRAEEKTRGQQAGLLVGTGVACVTKDYGSG
                     ADCSQASVEIDPAGRIAIHGDHVDMGNGIATALANRVAAHLGDVADEVTVHDVEAYGP
                     LALVTSGNSYTMNQATQDAAGKNPRWVPAISSATFASNGAHVGTQAAAEAARVIFRFG
                     LWPAALELWGIAPTHPTAKQWEAARWKDGHLLMPGLAPLSLPMIAAKAHARNFVTGAM
                     AHAFSRWGWSRATFPVAGQTWTADIDALAVRRGGGKYARLDRSSVKFPPTDNNRFGTD
                     YTALCATLVRVEIARATGVLRIAKAYSVFECGKALVPEIVLGQAQGGFAMGVGYTLLE
                     SLPPYEGGPGNGQWNLGQYLIARGSDLPLRDLEIEVLPPLTPDEPPKGIAEVVMIPVV
                     PAILNAIFDATGRRFRSLPVTQAMIKGALA"
BASE COUNT         2921 a         4633 c         4723 g         2844 t
ORIGIN      
        1 gaaatccagc cgccgtcgtc gataccggcg ccgatcgcgc cctggatgtc ggccagcgag
       61 gcattgacga tctggatgtt gagcaccgcc atgaaggcgc cgagggtggc gccggccacc
      121 gcaagccaga tcttcgacga gaccgcgggc tcggcctgat cgggcgcgct cgttgtttgg
      181 gcgcgatcgc cgctgacgtc ggcggaagca ggagggtcaa tggtggactg gagcgcggtc
      241 atgtaggtct cctcttgttg gcagaggcag agtgcattag gcggtgaatt tcgataatcc
      301 ggaaaactgg aaggattatc cagtagggct tgatgatgcc ctgccggatc cgctgcccgg
      361 gtctagctgc ccggctgctt gacgcggttc gaagccaggc gggttgctga ggtctgctgg
      421 tcgtgctcag cgagcaccgc cgccttggta ttgacggtcg gttccgccga catgcccgga
      481 cgcagcaggc cggtcaggtt gtgatcgtcc agcacgatct tcaccggcac gcgctgcaca
      541 atcttggtga aattgccggt cgcgttgtcc ggcggcagca gcgcgaattc gagaccgctg
      601 gccggagaca ggctgtcgac atggcccttg agcttggtgc tgtggaaacc atcgatccgg
      661 acttcgaccg gctggccatt gcgcacatgc gtgagctggg tctccttgaa atttgccacc
      721 acatagaccg catcaagcgg cacgaccgcc attaactgcg tgccagcctg tacgaattga
      781 ccgacacgga gcgaacgggc gccgacggta ccgtcgaccg gagcgatgat cttcgtatag
      841 gacaaattga gcgccatctg ccgctcgacg gcccgcgcgc gatcgacctg tgctgccgcc
      901 ttggctcgtt cggtggtgag aacgtcgact ttcttctgcg cggcgagcag gcccgatttg
      961 ccgtgctgta actgcgctgt cttctcgcgc agcgccgcgt cagtctgctg ggcccgctgg
     1021 atggtgccgg aaccggtctt catcaatccg tcgtagcggg cctgctcctc ctgggcgaat
     1081 ttgaggttgg cctcggcggc ggcgacatcg gcgctttcct gctcaatgat gggctgctgc
     1141 aaggcgatct gcgcgtcgag attgcgtacc gcggctcctg aagcatcgac gtccgcatgg
     1201 gcctgggcca acgcagcctc gaaatcgcga ttatcgatcc gcgccagcaa ctgtccggcc
     1261 ttcaccgact ggttgtcgcc gaccagcact tccgcgatat agccggagac tttgggcgcg
     1321 atgatcgtgg agtcggcctt gacgtaggcg tcgtcggtcg cttccaggta cctcccggtg
     1381 gtgagatagc cgtagccgaa gtcggccgca ccggcgacgc cgagggtcag cgccaacgcc
     1441 agggcggcgc gcttgattgc ctgccgggac gggcgaaggc tgattttggt atttgaatca
     1501 gagacataag aagcggtggt catggcatcc tcctgaatgt tggcgtccgt tgggagggcg
     1561 ccgtgatgtg cccccaacat gcaatgtccc gtgagatgtg ataattggga aaaaactgga
     1621 aacattatcc aatgagagcg gataatgaga ggagggccag tggatcggct gaccagcctg
     1681 acggcgttcg ttcgggtggt ggacagcggc gggttttcag ccgccggccg ccgcctcaac
     1741 atgtcgacca cgatggtgag caaccacgtc caggcactag aagaccggct cggcgcgcgg
     1801 ctgcttcacc gcaccacgcg gaaggtcagc ctcaccgaag tcggcaaggc ctattacgat
     1861 cgctgcatcc agattcttgc cgacctcgag caggccgacg acatcgccgg cgccttgcaa
     1921 tcgacgccgc gcggcacgct acgcgtctat accgccacgc atatcgttcc gttcatcgcg
     1981 ccagtggttg ccggatttct cgcctcctat cccgacgtca aggtcgatct gaccatgggg
     2041 gagcgaacta tcgatatgat cgacgaggga tacgacgttg cgatccggct gacgccgccg
     2101 cccgattcaa gcctgatagt ccgcagcctc gccacctggc ggcatgtgct gtgctgttcg
     2161 cctggctata tcgacaagca cggccggccg cagcaattgt ccgaattggc aaatcataat
     2221 tgcgttcgtc acgcacttta cccgtatgag gatgagtggc gctttgtcga tcggaaggat
     2281 acaccgactt cggtgcgggt ctcgggcaac ctgttctcca acagcggcga cacgctgcgg
     2341 acggccgcgc tgcagggtct cggcgttttc ctggcgccgg gattcctgat cgccgacgat
     2401 ctcgaggcag gccgcctggt tcggctcctg cccgagtacc ggccggtgga atttgcgatg
     2461 aacgcggttt atccgcaccg acatcatctc tcggcgaagg tgagaacctt tatcgatctt
     2521 ctcgcgctcc acagcgccga gcatcagaag ctgatcattc catatgcttg aatgcgagcc
     2581 gcaacgcgcc gcagtccgta ttgctaacgt tgaaggcgtc gtctcgatcg aagggcttag
     2641 cggcaacgag gtggaggtgc cggctagcgg cccgccttgt ggtcttcaat aggagcgtcg
     2701 tcatcctgga acaggatacg ctcggccgcg ccgtcgagat cctcgtactg accgctccgg
     2761 agcgaccaca gaaaagcggc taggccggtg aggccgagtg ccagcgcgat cggtacgaga
     2821 taaagaaagt cgatcatcga tgactgccga aggcgatgtg cgaggcgata cgttggcctg
     2881 cgtcaatggc cttgtcctgc tgcaggcccg cgcggcgccg attgatgcgc aatgcattgg
     2941 caatgacggc aatcgaggac accgacatcg ccacggccgc gatcagcggt gtcacatagc
     3001 cgcaaaccgc gaccggtatg gcgatcgcgt tgtatccaat cgcaagagcg aggttctggc
     3061 ggacgagatg cttggcttcg cgcgccaccg caatcgcctg cggaacggcg aacaggcttt
     3121 cgtgtaggaa caccaggtct gccgcattgc ggccaatgtc cgcagccgat gccggcgcca
     3181 tcgaagcatg agccgatgtc agcgccggcg cgtcgttgag gccatcgccg gccatcagaa
     3241 ccttcctgcc ggcggagcag agcgccttga tgcgggcaac tttttctcgc ggcgatacat
     3301 tcgcacgata gtcaaggccg agagctgtgg cgagctcgct cactggctgc tcgcgatcgc
     3361 ccgacagcac ctcgactggc agcccttcgt ctttcagggc ggcaatcgcc tcacgcgcat
     3421 cgcggcgcaa gctgtcatga agatcaaagc cggccagata cgcgccgtcg cgcgataaca
     3481 cgacctcgga tcgtgcggag accggcaggg ctcgatcgcc cgaaagcgcc cattcacggc
     3541 gaccgagccg atagattgtc gtgccggcgc gcacttccag tcccgatccc ggcaattcgg
     3601 acacatcggt gaaggcagtg aacgcggcgt gcacctggtc gttgcccgca accgcgagcg
     3661 cgcgcgaata gggatgccgg gagtgcgacg cgatcgcgcc cgcgagcgtg agcacctccg
     3721 gcggtatcgc atcggcattg actagttggg gattgccaag cgtcaacgta ccggtcttgt
     3781 caaaaaccac ggtatcgatc tcggccaggc gttcgagcgc gctgccgtcc ttcaccatga
     3841 tgccgttctc gaacaggcgc gccgcggcca ccacctggac catcggcacc gcgagcccga
     3901 gcgcgcaggg gcaggtgatg atcaacacgg caatggcgat cgtcaccgcc tgatgcagat
     3961 cgccggctgc ggccatccag ccgatgagcg tcaacagcgc ggtcagatgg acgacggggg
     4021 catagagcga ggcggcacga tcggcgatcc ggcgatagtt cgagcggccg ccttctgcca
     4081 cctccatcat ccgcgtcatc tcggccagga acgagtcctt cgctgccgct gtcgcgacaa
     4141 tggtcagcgg tccggtcagg ttcattgtgc cagcctgaag cagcgcgcct tcaacgacgg
     4201 gctgcggcag gctctcgccg gaaaccagcg aaacgtcgag ttcggaacgg ccgcttgtca
     4261 cccggccgtc gaccggcacg cgctctccgg ccgcgagcag aatattcatc ccgggcgcta
     4321 tctcgttcac cggcaggtag acccgtgctc catccggctg caggaccagc gcgccgcgcg
     4381 ccgccagacg cgcgagcccc ttgacggcag agcgcgcccg ttcgcgcatc atgtgatcga
     4441 gggtccggcc gatcagcaga aagaacacca gcgaaatcgc ggcatcgaaa taggcgtgcc
     4501 ggccgccctg gaatgtctcg taaagagaca tgccggtggc gagcaggatg ccgacggaga
     4561 tcgggacatc catgttggtg cggccgtgcc ggagactttg ccatgccgaa cggaagaaca
     4621 cgcggccgga ataggccagt gcgggcaacg cgatcagcgc cgacaaccag tggaacatgt
     4681 ctcgcgttgc cggttcggct ccggaccaga ccgaaaccga cagcagcatg atgttgctgg
     4741 ccgcgaaacc ggcgaccgcg agtgcgcgta ccagctcacc ctgaacggcg tccttccgat
     4801 cgccgtcgac gtcgaagagg tgggccttgt aaccgatcct gtccagcgcc tcgatgaagg
     4861 gcggcggatc gccgccacgc cagcgaaccg tgactcgctt ggtcgagaga ttgacgcgcg
     4921 catgctcgat atccggcaag gctccgagag ttctctcaat cgcctgaatg cagccgccgc
     4981 agtgaatacc gggcaccgaa agatcggtct gaagcagatc ctcgctgata cggtgactcg
     5041 ccagcaaaac ttcctgccgc gcaacggcgg ggtcggtcct cgcaatgaaa gcctcggcgc
     5101 cggctgcgca acagctcatc gaacactccg tttcggcgcc cgcttcgagc ggattcggcc
     5161 aaatcgccta tcgagccacc gcctgatagg cgtgccaggt gccgtgtccg agcagaggaa
     5221 acacgacgat caagccgaca aggccggtcg caagactgaa aaggaacagc gccagcacga
     5281 tcgcgcccca ggtcagcatg gccggcagat tgttccagac cagcgccatg ctcgatccca
     5341 tcgcggtcaa ggcatccgtt ttttcgttga gcagcatcgg aatcgagaac gcgctgatcg
     5401 caaacgagaa cgatgcgaat aatccgccga ccagaccgcc tacgatcagc atggcccagc
     5461 caacctcggt cttgaacagc atcggtacga tttggtcgag gcctggaaac ggcagcaggc
     5521 cgaagaaaag cgcataaatc aggacagcgg cgcgcatcca cactagcatc agcaggcaga
     5581 gcagtacgcc ggtaaacagg atctggccgc ccgacttggg gtgaacgaac aggatgtcat
     5641 tccagcttgt cggctcgccc gccgcgatgc gccggctttt ttcgtacagg ccgaccgcga
     5701 ggatcggacc gatgaccatg aagcctgcga gggcggggaa aaggatgtag tcccagccaa
     5761 gcgcgtacag cccggcaacg atcgcaatcg agatcagaaa gacgatgagg ccataaccga
     5821 ggctggtgac cggctgaatc caaaaatcct tccagccatc ggagagccat tcgaatgcgt
     5881 cggccggctt cagcgaccgg ctccatttgt tttcggttgg cagcggcggg actaccgaag
     5941 ggatcatggt catgtgtctt gctccaccga tcctattttt gagcgattga tcgcggcgaa
     6001 catgacgatt gcgctgcgct gaatgggcgg tgcgggaggc cgccttcgcc ttgcctgaca
     6061 tgggcaacgc agtccggctg cgagatcgtc gccacataga ccagatgcac attcgcgagc
     6121 accaccagca acaccccggc gctgaccgaa agcgagacgg ccactttcgt cctccgggac
     6181 cactgcatca ttgacggctc agtcattggt cgggtgctcg cttgtcgacc agataaagcg
     6241 ccaggatctt gcgatcgagc gccgacaatc gtccttccca tgtcggcata tggccctgcc
     6301 ggccgcccca gaccgacgtg tcgatggttt ccagattgcc gccgtagatc cagagcttgt
     6361 cggtcaggtt gggcgcgccc agttcgaggt tgcccttggc gtccgcaccg tgacaggaga
     6421 cgcagttagc ggcaaagatc gctttgccgg cctcgaccac cttcgggtcg gccgtgccgg
     6481 agttcggact agacaacgaa tggacgaagg tcgcgacttt cagaacgtcg gcgcgtggaa
     6541 tcatctgatc ccggccgaaa gcgggcatct gcgacacccg cgttttcgga tgggtcgaat
     6601 tgattccaac ccggatcgtt tcaaaaaccg cctcgggcgt accgccccac agccaggatg
     6661 tcgtggttag gttcggaaaa cccttgccgc ccttggcgtc gaggccatga caaacagcac
     6721 agttatcgcc gaacaaggtc cggccggatt ggcgcactac ggccatcagt cgaggatcgg
     6781 actgcactgc cgcaaaagag tcgccctcga tcttcttggt ccagacatct cgagcgagcg
     6841 tcgcctgctc aagtgatgct tcaaccacct ggcgctgatc ggtatggagc aatcccttgg
     6901 taaaggtagt gccgagcggc caggccggca tcagcaccca gtaaccaacc gaaaacagga
     6961 aagcgagcga gaagaagaaa tagattaagc gcggaacagg ggtattcagc tccttgatgc
     7021 cgttccactc gtgaccggtc agcatgtggc cggtgtaggt gtcgcgttcg tctagcgcca
     7081 tggccggtcc tcgtcgctga gaatggactt cgcggcccga tcgaactgct tcttgttgga
     7141 aggccagtaa acatacacaa gtacaatcac cgaaagcgcg atcaggtaga acagcccgaa
     7201 ggatttcgag aaccaggcca gcgtgtcgtg ggcgacgttc atcgtcactt ctccgtggat
     7261 gcgaccggct tgtaggccgc gtcggtgagg tgtccgagaa cctgaagata agccacgacc
     7321 gcgtccattt cggtcagttc attcggccgg tcatcgaacg cgcgcatgtt cgttgccttg
     7381 ccgtaacgct tgacgacccc ggcggcccag ggcgaatccg gcgaagcctg gccataggca
     7441 tcggttgcgg cattggcgat catgtcgtcg gtatacggca ccccgacggc gcgctgcgcc
     7501 ttgagatgaa ggccgagatc gtcggttcgc agggaggtgc gaagcagcca ggtataggcc
     7561 ggcatcaccg aaaccgacac cacgtcgcga ggattgttga ggtgagccgc gtgccactgg
     7621 tccgaatatt tctcaccgat ccgcgcgagg tcagggccgg tgcgtttcga tccccagagc
     7681 atcgggtggt cgtatttcga ttcaacggcg agcgaatagg ggccgtagcg ttcgacctcg
     7741 tcgcgcaggg tgcggatcat ttgcgagtgg caggcgtagc atccttcgcg gatgtagatg
     7801 ttgcggccgg ccagttcgag cggcgtatag aggcgcatgt ccggcacgtc ttcgacggtc
     7861 tgatggatgg tgaagagcgg cgcaatttcg accaggccgc cgatgctcgc aacaccgacg
     7921 attccaagcg cgaggccgat tgccttgcgt tccagccgat aatgaaagcc gaacatggcc
     7981 tactccgcct gctgtaaagc tgcgccggcc ggcagcaatt cgggtcgatc ggccagctcg
     8041 tcggcggtct gcgataccat ccggatcgtc atccagatgt tgtaggcgcc gattaccgcg
     8101 ccgatcagaa acaacaatcc gccaaccgcg cgagcgatgt agtaggggtg cattgcgatc
     8161 agcgagtcga tgaacgaata ggtcagggtg ccgctctcgc tataggtccg ccacatcagg
     8221 ccctgaacaa tgccggaatt ccacatcgca aaaacgtaga ttatggtgcc ggcgacggcg
     8281 aaccagaaat gcgcctctac cagcctcggc gagtacatgg ttttgcattt ccagagccac
     8341 ggcaccgagg catagatcga accgaaggtg atcatcgcga cccagccgag cgcgccggca
     8401 tggacgtgac ccacggtcca gtcggtatag tgcgacagcg aattgaccgg gcggatcgcc
     8461 atgaacgaac cttcgaatgt ggtgagaccg taaaacacgg cagccaccat catgaaacgc
     8521 agcgtcgcgt cgtcacgcac cttgtcccag gcgccgttga gcgtgagcaa ggcgttggcg
     8581 gccgacgccc aagaggggat cagcagcatc accgagaatg tcatgccgag cgtctgtacc
     8641 cattgcggca gcgccgtgta gtggagatgg tgcgagccag cccacatgta cagaaacgtg
     8701 atgccccaga agctgatgat cgacagccgg taggaaaaca gcggccgccc cgcttgcttg
     8761 ggcagaaaat aatacatcat gccgaggaag ccggcggtca gaaaaaaggc gaccgcgttg
     8821 tggccatacc accactgtgt catcgcgtcc tgcacgccgg agaacgctga atagctcttg
     8881 gaatggccga gcgagactgg aatggcgagg ttgttgacga tgtggagcat cgcaacgacc
     8941 agaatgaagg ccaaatagta ccagttggcg acgtagatat gcggctcctt gcggcgcgcc
     9001 agcgtttgca aatagagcac caaataggtc acccagacga tcaccaagag gacgtccgcg
     9061 taccattccg gctcggcata ttccttcgac tgcgtcaccc ccatcatgta tccagtgacg
     9121 gcaatgatgc agaacagatt gtagccgaac agcacgaacc atggggttaa ctggcccgcc
     9181 agtcgcgcac gcgatgtgcg ttgcatgaca tagaaggagg tggcgatcag tccgttgccg
     9241 ccgaagccga aaatcacacc ggtggtatgg accggacgaa gccgtccgaa gctagaccat
     9301 gcggcatcga aagtgaggtc agggtaagcc agaagccacg ccacccagtc gccgacgaac
     9361 atgccgaaca cggcccaagc catcgccagc acgattccga ccttggtcgg atcgtcataa
     9421 tagtggcgag aacgatcctc attcggctcg ggctgatagt agtcggtaat gacacggaag
     9481 gtggcgacga tcgccagcag cgcgaccaga gcgccgtgaa tgcccagcgg atcgttccgg
     9541 cctactatcg ctacaacaag gccgaagacc gctgttatga ccagcatcac aagcgcgagc
     9601 tgccgttcgg atctggtgag ctgggcaatc atgtcggaca tcctgccgtg ctatccgttg
     9661 agaggcggcg agccgggtca agaacggccc gcccgcgatg caaaatgggc cgatagccgg
     9721 agccatcggc ccatggcgcc cgtgcgagta tatgcaatcc gaggggcaat tgcataggta
     9781 ctcttgttcg gccagaaatg ctcgggagag gaagccattt aatcgaccta caggtatcgt
     9841 ttcgttgcga agtacaaagg aaaaatttat gctagtccgc ggcttgcttc ttgcaagttc
     9901 agggcgcgct tagaacatac ctgccatagc gatctacacc tcgagttgcg tcctccttgg
     9961 agctagcttt gggggcagcc actggattcc aacagcaatt gggattggcg ggatcaatcc
    10021 ttccgcccct gttcaagtcg caccgataat attcttgcgg cacgccccgg caatggccgc
    10081 gcgtgcgtcc cccgccgccg tgatcgtccc gaccgccagc gcggccaggg gcccgaggat
    10141 cccaaagcgc ttgatgtatc tccttatcca ccccgtcgtt cattaccgcc caaatcacgt
    10201 tatttgtagt ttcacttgtg ggggaattgt gccgtttctc gcgcgctgcc cgatcgcaaa
    10261 gtgttaggct ttggccatca cgtcgtcctg agggcagggg gagcgcaaca tgagacggtg
    10321 cggattcatc attcttctgg ctaatcacac tgggcccggt gccaagatct tggtgaacct
    10381 tccatgaatc tcaacactat tgtcgaggtt ttaatacgga aggcagggca gttcggtttc
    10441 cccgccggaa tggtgctggt gcttgccatt cactctcccc ctgcgacctt tgcgcaggat
    10501 ggaatcgatg gggtcactgg cttcgcgcgc gatccggttc atgtcgccgc ctggccagcg
    10561 ggcagaaagg tcgcggtcag cttcgcactg ttcgtcgagg agttcggctt cggccagggt
    10621 ccggtcttcc gccctgacct cgcgagccgc aatcccgatc tcgtgaacga ggctttccgt
    10681 caatacgcga tcgactgggg catcccccgc gtcggccggt tgttcaggga attggatgtt
    10741 ccgctcagca tcgtgttgaa cgccgaattt cccgctaccc acgcgtcggt atggaaggaa
    10801 ttccgcgcga cacagccgaa tgcgccggtc gtcgcccacg gcatgaacaa cagcagccac
    10861 atgctgccgc tcgggcgtgg gctcgccgaa cagaaggctt acattcgccg gacgctggag
    10921 ctgatcgccg ccacgaccgg cgtcaaaccg accggctggt cgtctcccag cgtctattcc
    10981 aacggcgaca cgatgcaggc gatggccgcc gagggcatcg cctacaacct cgaccagatg
    11041 gattccgaca tcatttcgcg cctgaagacg ccggacgggt cgttggtgtt gctgccctat
    11101 ccggtcgtta ccgtcgacat gggccagaat ctggcgcgaa tgaagacacc ggcggagatc
    11161 gaggctctgt ggctggatta cgtgtcggag ttggcgcgcg aggcccgtgc caaccctgcg
    11221 cgagaagcaa cgacagtggt catcggaatc cacccctttg taatcggcac gcccgacggg
    11281 gcggccgcct tgcggcgtgt tctcctgcgt ctcaagagcg atgacgcggt ctggctcacc
    11341 gacaccgacg caatcctgaa agcagccgcc ctgaaatgat ggtgggtttg cgtctcggag
    11401 gggcggcatt ttcgttaagc atggtctaag ctgcctctga agattcgtct agtcgaagtg
    11461 tcctccctgc aatcgggcaa aaagatggaa ttcaccgtat tgttcctgtg tgtcctcgtt
    11521 gtgatggcgg tgtcgtggcg gggctcgcat cgcctggcgc tggcactgtt ttcgatcgtg
    11581 ctcgtcgcat ctgtggcaac ctatctccac catgcgaccg acaccttgaa tctttcgttc
    11641 tagggacgtc tctcgtttta ggcatgcagg catgaaggca tgaaacgctc gatcggcgtc
    11701 accctcaatg cacttggtct ctacggcatc gcgctggtcc tcctcgctgc cttcgcgggt
    11761 cagattttgc tcggcgagct gccgtgtccg ttgtgcctcc tgcagcgggt cctgttcgca
    11821 atgctggcga tcgggcctat cctgaatatc cgcttcggtc cgcatcccgg tcactatgcg
    11881 ctttcattgc ttgccgccat tgtgggcgcc acggtctctg cccgtcagat cctgctgcac
    11941 atcatgccgc gggatccggg gtacggcagc gcgcttctcg gctatcacta ttatacgtgg
    12001 gcgttcatcg cattcgccgc cgccatcgcc ctgatcgcga tcgtgctgct gttcgacgat
    12061 cggtcgcccg ataacaaggt ggcgggtccg gtcggactat ttgcgagcgc tgcggtatgg
    12121 ctggtgatca gtctgaccgc gatcaatgcc gcctctacgc tcctggagtg cggctttaaa
    12181 gcctgtccgg acaatccggc gttttacgaa cttctcaagc ggtcccctta gagcatgatc
    12241 cggaaaactg ggaaccgatt ttctgaaaag atcatgctca aaacaaaaag atgagatcat
    12301 gatccgatgg atcatgatct agccgcgcgt ttcgatcaat tgcctgggca aattgctccg
    12361 ctctttaccc aggcttcgac cagcgctccg gcctccttct gcgtgcccgg agcgggggag
    12421 cgcccatagc cgggcgccca ggcccagccg accagggtgt cctcgccaat gtgatggatg
    12481 aggtcttcgg gcttgcggcc gccattgcgc gcagggtctt tgatctgcgc gcagatctcg
    12541 ccaacggcct tgccctccca cgccatctcg cgtggcgcga gatgccaatc ggggtggcct
    12601 ggcacgcggc cggggtcgaa gttcgcattc tggtgacaga tggaacagcg catcgccggc
    12661 aggccgcgac cgtccgcacc gcgttcgacc ggcggctgat gaagccggcc ttcatttgcc
    12721 tgatgtgggc ggtcgccggc gggatggcaa ttaacgcagc gcggatgggt cagcaccttg
    12781 ccgagctccg tgaacatggc ggccgagcgc gcttccgggg cgttgatcgc cccgaaactc
    12841 tcgggtgaag ccagtgtgtt ggccgaggtt tgcgaggccg cgtaactcgt cagcacgctt
    12901 gtcgtgaggg ccgcgaccgc aatcaatatc ctgacccgca tctcggattt catcgtcggt
    12961 cccctaatgc cgtcaatgcg cgggatcatg cactacaaga ggtagctttt cggatcggcc
    13021 aggatcacgt cccgcacggc ctcatgatat ttgatgtagc cggtgcaacg gcagatgtgg
    13081 ccgtcgagcg cctcggcgat ggttgtctca agtttggcgc gcgccaccgg ggtctttgcc
    13141 agccgctcca gcagaacctg gccttcattc aggaagccgg cggtgcagta gccgcactgg
    13201 aaagcgaagt ggtcgatgaa agccttctgc agatcggaga gctcgccgtt cctggcgtgg
    13261 ccttcgacgg tgcggatcgc cttgccgtcg aagctcgctg ccggaacgat gcaggtcggg
    13321 ctggtgtaac tcgtcccgtc gggcctgtcg acgatcaccg cacagctcaa gcactgcgcg
    13381 gcgccgcatc cgaacttggt gccggtcatc ccgaggtact cgcgcaagaa atcgttcatc
    13441 gtcaaatcgt cgcgcacctc ggtcggaccg taggcgcggc cgttgatcgt gatggcgagt
    13501 gtggtcacgc gagcgctcct ttgatcatgg cttgagtgac cggcagggac cggaagcggc
    13561 gaccggtcgc atcgaagatg gcattcagaa ttgccggaac gaccggaatc atcacgactt
    13621 ccgcaatgcc ttttggcggc tcgtccggcg tcagcggcgg caatacctcg atctcgaggt
    13681 cgcgcagcgg caggtccgac ccgcgggcaa tgagatattg tccgaggttc cattgcccgt
    13741 tgccgggtcc gccttcgtaa ggcggcagcg attcgagcaa ggtgtaaccg acacccatgg
    13801 cgaagccgcc ctgcgcttgc ccgagaacga tttccggcac cagcgccttg ccgcattcga
    13861 acacgctgta agccttggcg atgcgtagta cgccggtagc gcgcgcgatc tcgacgcgca
    13921 ccaaggtggc gcagagtgcg gtgtaatcgg tgccgaaacg gttgttgtca gtgggcggaa
    13981 attttacgct cgagcgatcg aggcgcgcgt atttaccgcc gcccctgcgc acggccagcg
    14041 catcgatatc ggcggtccat gtctgcccgg cgacggggaa ggtcgcccgc gaccagcccc
    14101 agcgggagaa ggcgtgcgcc atggccccgg tcacgaaatt gcgcgcatgg gctttggcgg
    14161 cgatcatggg caacgatagc ggcgccaaac ccggcattag caggtgtccg tctttccagc
    14221 gcgcggcctc ccattgcttg gccgttggat gggtcggcgc gataccccac agctcgagcg
    14281 cggccggcca taaaccgaag cggaatatga cgcgcgcggc ctcggccgcc gcctgcgtgc
    14341 cgacatgcgc gccgttcgag gcgaacgtgg cactactgat cgccggcacc cagcgcggat
    14401 tttttcccgc ggcgtcctgg gtcgcctggt tcatggtgta ggagttgccc gaggtgacga
    14461 gcgcaagcgg gccataggct tcgacgtcgt gcacggtgac ttcatcggcc acatcgccca
    14521 gatgggccgc cacccggttg gccagcgccg tggcgatgcc gttgcccata tcgacgtgat
    14581 cgccgtggat agcgatccgg ccagcagggt cgatttccac cgatgcctgc gagcagtccg
    14641 cgccggagcc gtagtccttg gtaacgcaag ccacgcccgt gccgaccagc agacctgctt
    14701 gctgtccgcg agttttttct tcggcgcgct gctgccagat cggatgcttc tccagcttgt
    14761 ccaagatctc gggcgtgcgg atggagacga tgtacggatt tcccgtcatg gtcttgccgc
    14821 cggtcttgag cgcgttgcgc cgccggaact cgatcgggtc gagcggcagc gccgccgccg
    14881 cctcgtcgat cagcacctcc agcgccgtca tcggttgcag cgtgccgtaa ccgcgcatcg
    14941 atcccgcagt aacgccgcgc gaatgcagcg cgaccgtggt cacatcgacc ttcggaatgt
    15001 cgtagatgcc gatggccgcg gcggcgccga cggtcgcaac gttggctgaa aaattggcga
    15061 ggccgccgcc atccagcacg tgatcggcgg cgaatccact gatcttgccg gtcgcgcgat
    15121 c
//
LOCUS       DATNOI010000006         2169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1238340,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000006 DATNOI010000000
VERSION     DATNOI010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238340"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VN968_00180"
     CDS             <1..666
                     /locus_tag="VN968_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL27700.1"
                     /translation="TAVSRDGPTFYFLCLAVLVLTIVGTLNLLRSPTGRAFVAIRDSE
                     TAARSMGINVSLYKVKSFAISAAITGFAGVLFAHKLSFISPEMFTLQLSIEFIIVILI
                     GGAFSPHGAVLGAIFIVMIDPFLTYLKDDVPGLVAGVAATLGAGKESVAHIQSNVAAF
                     ASANGLKGAIYGIIIVLFVLFEPLGLYGRWLKIKLFFQLFPLYKRATFKRQKIYVKSE
                     RNR"
     gene            663..1421
                     /locus_tag="VN968_00185"
     CDS             663..1421
                     /locus_tag="VN968_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL27701.1"
                     /translation="MSYFRAENLSLHFGGLKAVDAVSFAVEKGEILSIIGPNGAGKSS
                     IFNLISRIYSPTSGRIYFEDQDITREPAFGIARLGIARTFQNIELFENATVLGNLLVG
                     RHRHSTTQLWQELLFLPSVRSNEKIHRRRVEQVIEFLDLEPYRDKLISGLPYGVRKVI
                     ELARALCTEPKLILLDEPSSGLNVEETDDMSFWIRDMKTELGITVLMVEHDMTLVNRV
                     SDRVIALNYGKVLAIGSPAEVQRHPDVVAAYLGT"
     gene            1433..>2169
                     /locus_tag="VN968_00190"
     CDS             1433..>2169
                     /locus_tag="VN968_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL27702.1"
                     /translation="MSAPDIILRLSNIESYYGPIMAIKGISVEVPRGKIVTLLGANGA
                     GKTTVLKTISGILDPQKGSIEFLGKPIQRMEADKIVRLGLSHVPEGREVFPFLSVREN
                     LMMGAYPRKDRDAVAEDLERVYGYFPRLKERLNQAAGQLSGGEQQMLAIGRALMNRPA
                     LLLLDEPSLGLSPILVKEIFTIIRRVNEEQGMSILLVEQNAKVALETAHYGYVLEIGR
                     VVMNDSCERLLHSKDIQEFYLGAKEAGA"
BASE COUNT          418 a          661 c          654 g          436 t
ORIGIN      
        1 accgccgtgt cgcgcgacgg gccgacgttc tactttctgt gccttgcggt acttgtcctg
       61 accatcgtcg gcacgctcaa tttgctgcgc tcgccgaccg gccgcgcctt tgtcgccatc
      121 cgcgacagcg agacggcggc gcgcagcatg ggcatcaatg tctcgctcta caaggtgaag
      181 tcgtttgcga tcagcgctgc gattaccggg ttcgccggcg tactgtttgc gcacaagctg
      241 tcctttatca gcccggaaat gttcacgctg cagctctcga tcgagttcat catcgtgatc
      301 ctgatcggcg gcgcattcag cccgcacggc gccgtgctgg gggcgatctt catcgtcatg
      361 atcgatccgt tcctgaccta cctcaaggac gacgttcccg gcttggtcgc cggcgtcgcc
      421 gctacgctcg gcgcggggaa ggagagcgtg gcgcacatcc agagcaacgt cgccgcgttc
      481 gcttccgcca acggcctgaa aggcgcgatc tacgggatca tcatcgttct gttcgtgctg
      541 ttcgaaccac tcggtcttta cggccgctgg ctgaagataa agctcttctt ccagctgttc
      601 ccgctctaca agcgcgccac cttcaagcgg cagaagatct atgtgaaatc ggagcggaac
      661 cggtgagcta tttccgcgcc gaaaatctgt ccttgcattt cggcggcctg aaagcggtcg
      721 acgccgtcag cttcgccgtg gagaagggcg agatcctgtc gatcatcggg cccaacggcg
      781 ccggcaagag ctcgatcttc aacctgatct cgcggatcta cagcccgacc tcgggaagaa
      841 tttatttcga ggatcaggac atcacccggg agccggcgtt cggcatcgcc aggctcggca
      901 tcgcccgcac cttccagaac atcgaactgt tcgagaatgc gacggtgctc ggcaatcttc
      961 tggttgggcg gcaccggcat tccaccacgc agctctggca ggagttgttg tttctgccga
     1021 gcgtgcgcag caacgaaaaa atccatcgcc gccgcgtcga gcaggtgatc gaatttctcg
     1081 atctcgagcc ctaccgcgac aagctgatct cgggactgcc ctatggcgtg cgcaaggtca
     1141 tcgagctggc ccgggcgctg tgcaccgagc cgaaactgat tttgctcgac gagccgtcgt
     1201 ccggactgaa tgtcgaggag accgacgaca tgtcgttctg gatccgcgac atgaagaccg
     1261 agcttggcat caccgtcttg atggtcgagc acgacatgac actggtgaat cgtgtttccg
     1321 atcgcgtgat cgcgctgaac tacggcaagg tcctggcaat tggatcgccg gccgaggtgc
     1381 agcgtcatcc cgacgtcgtt gccgcttatc tcggcacctg aaggatgacg gcatgagcgc
     1441 tcccgacatc atcctcagac tcagcaacat cgagagttac tacgggccga tcatggccat
     1501 caaaggcatc agtgtcgagg tgccgcgcgg caagatcgta accctgctcg gcgccaacgg
     1561 cgccggcaaa accacggtgc tcaagacgat ctccggcatt ctcgatccgc agaagggctc
     1621 gatcgaattt ctcggcaagc caatccagcg catggaagcc gacaagatcg tgcggctggg
     1681 cttgagccat gtgccagagg gacgcgaggt atttccgttc ctgagcgtgc gcgagaattt
     1741 gatgatgggc gcctatccgc gcaaggatcg cgatgcggtc gcagaggatc tggagcgggt
     1801 ctacggatat tttccgcggc tgaaggaacg actcaaccag gccgccggtc agctttccgg
     1861 cggcgagcag caaatgctcg cgatcggtcg cgcgctgatg aaccgcccgg cgctattgct
     1921 gctcgacgaa ccctcgctcg ggctgtcgcc gatcctggtg aaggagatct tcacgatcat
     1981 ccgccgcgtc aatgaagagc agggcatgtc gatcctgctg gtcgagcaaa acgccaaggt
     2041 cgcgctggag acggcgcatt atggttacgt tctggaaatc ggccgcgtgg tcatgaacga
     2101 cagttgcgag cggctgttgc actccaagga cattcaggaa ttctatctcg gcgccaagga
     2161 agccggcgc
//
LOCUS       DATNOI010000007        11246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1783074,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000007 DATNOI010000000
VERSION     DATNOI010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783074"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VN968_00195"
     CDS             complement(<1..963)
                     /locus_tag="VN968_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HXL27703.1"
                     /translation="MIEQQLGRCVASWCPGATGVIGAAKLSGGASQDTWSFDILHPDG
                     VFGAILRRAPPGHGAAPSRAAGLNAEAVLMQLAHEAGLPSPRVLHVLQPQDGLGTGFI
                     MQRVEGETIARKILRDEKFASARPHLARQLGKVAAGIHGLPLAKLPKLRRMTSAKEIT
                     ELDRDYRGFDWPRPVFELALRWLRDRDPGPSQEVTLVHGDFRNGNLIIGPDGLRAVLD
                     WELAHTGDPMEDLGWVCVNSWRFGEIDKPVGGFGTRDELFAGYEAAGRRVDPERVKFW
                     EVMGTLRWGVMCCGMMQRFRSGPEHSIERAMIGRRSSETEIDLLR"
     gene            complement(960..1754)
                     /locus_tag="VN968_00200"
     CDS             complement(960..1754)
                     /locus_tag="VN968_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase"
                     /protein_id="HXL27704.1"
                     /translation="MQFKHVTLDFDGALAILKLDHPEVMNAVSMDMLGGLGEALDAID
                     DRRNEVRCLVMTGAGRAFCAGANLQGRDNQKPGSRSNAGVTLETGYHPFLRRLRNLHC
                     PIVTSVNGAAAGAGMSFALMGDMILCARSAYFLQAFRRIGLVPDCGSTWLLPRLIGKA
                     RSVELSLMGERLPAEKALEWGLVNRVYDDTTLMEETMKLARELAEGPTIALSLIRRLY
                     WESSDNSFEDQLDLEQQMQRRAGAAEDFREGVTAFLEKRPAKFKGK"
     gene            2428..3036
                     /locus_tag="VN968_00205"
     CDS             2428..3036
                     /locus_tag="VN968_00205"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXL27705.1"
                     /translation="MEYVPDDLTPRERYKVLTSFVMPRPIAWVTSVGPTGVVNAAPFS
                     FFNVFCEDPPLCMFAVNLRPDGRVKDTLVNIQRSGEFVVNLTDEPLARAMHDSSGDFP
                     PDIGEPGYLGLKLAPSTKVAVPRLADTPWAMECKTWKTIDVNGNRQLIMGEGINFYIR
                     DELWDNEAMRVHMDRYHPVGRMFADRYCRTDDRVEFPAAQGA"
     gene            complement(3056..4726)
                     /locus_tag="VN968_00210"
     CDS             complement(3056..4726)
                     /locus_tag="VN968_00210"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL27706.1"
                     /translation="MSALQPISGKDHAAGGETYDVVVVGAGFAGMYMLHKLRGLGLTV
                     RVYEQGDGVGGTWYWNRYPGARCDVESMQYSYSFSDKLQQEWDWSERYAPQPEILKYA
                     NHVADRFKLRPDIALNIRIDQAIFDETANTWRVTTSDGNTVTAKYVVLATGCLSNARM
                     PDIKGLAGFKGKVYHTGHWPHEPVEFTGLRVAVIGTGSSGIQSVPVIAEQASHLTVFQ
                     RTANFSIPAHNAPLSQEEREAFRAKYPEVRRFAREETRNGIYTEMPDRGALDDGDNER
                     RTRYESRWAYGGLTFMAVYNDLALNKAANDTASDFVREKIAEIVKDPQTAKLLQPNNH
                     PIGTKRICVDTDYFATFNRPNVTLVDIRSNPIEEILPNAVRTAARDYEVDALVLATGF
                     DAMTGSVAKIDIRGRNGQTLNQKWAEGPKTYLGVMSAGFPNLFIITGPGSPSVLSNMM
                     VSIEQHVDWIADCIAHMRDRGLEAMEAEKQAEESWVAHVNEVAHTTLYPQANSWYMGA
                     NIPGKPQIFMPYIGGVGVYRQICNNVAAKGYKGFVMTAVEQPQKIAAS"
     gene            5066..6199
                     /locus_tag="VN968_00215"
     CDS             5066..6199
                     /locus_tag="VN968_00215"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXL27707.1"
                     /translation="MKSPICDMLGIEFPLLAFSHCRDVVAAVSRAGGFGVLGATSHTP
                     ETIEQELKWIDDHIDGKPYGLDVLIPENISTAGEKDVTWKSLEARISNEHRAFTKTLL
                     KKYGIELTTTSVADNRPQPFDGQCALEVLDASFKHPIRLIANALGVPPKAMIDKGRKH
                     GVPVAALVGAKEHALRQVAAGVDILVVQGTEAGGHCGEVSTMVLVPDVIKAIKPIRDV
                     PVLAAGGIMTGRQMAACMAMGAAGVWTGSVWLATVESETTEIFREKMIAASSRDAVRS
                     KGRTGKPARQLRSVWTDAWDRGSDSPGALPMPLQSIISRDAFNSIDRAAAAGNAQARD
                     LVSYFVGQGVGLIDSVKSAGAVVQEFKEDFADAVEHMNALVRE"
     gene            6354..7868
                     /locus_tag="VN968_00220"
     CDS             6354..7868
                     /locus_tag="VN968_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HXL27708.1"
                     /translation="MPANFVPDDRVPVIVGVGEIVDRPKEILEGLEPLALLEQALKRA
                     EADSGGKLLGEIGSLDIVNFLSWRYRDPEILLSDRLGIKPKHAYYGPVGGESPIRYLH
                     EAAQRIARGDCSVAAVCGAEAQSTATKAERAGVELPWTPFAHDVPEPKRGAAFQKPMA
                     VKLGVFRPITVYPFYEAATAAHWGQTPREAMAESGALWSTYADVASQNPNAWLRRRFT
                     SDEITTPTLENRLIAWPYTKLQVANPTVNMGAAVLMTSLAKARAAGIAEDRLVHVWGG
                     ASAEEPRDYLIRDQFFESHPQNAVLNAVLDLVGGNGKAFDAIELYSCFPCVPKMARRT
                     LGLGTEVQPTVTGGLTFFGAPLNTYMTHAACAMVRKLRSGANFGLLYGQGGFVTKHHA
                     LVLSRQAPRGALAQDTSVQTEADRHRRAVPEFVTEANGKGEVESFTVIYGRNGEAEHD
                     VVMLRTQDKARALARVPASDRAALSHLLDLDRSPVGSRGDIVTADDGVLEWRVG"
     gene            complement(7975..9252)
                     /gene="otnK"
                     /locus_tag="VN968_00225"
     CDS             complement(7975..9252)
                     /gene="otnK"
                     /locus_tag="VN968_00225"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HXL27709.1"
                     /translation="MTLALGCIADDYTGASDLANTLTRCGLRTVQTIGVPAGDLALPD
                     VDAVVVSLKSRSIEASLAVSRSRDAEKWLRKRGADHVLFKICSTFDSTDAGNIGPVMD
                     ALRADSGDAIVLVTPAFPETGRTVYQGNLFVGAVPLNESPLKDHPLNPMHDSNLVRVL
                     ARQSKAKVGLVDLADIARGPDAVRARLADLSGKGFGAAIADAVFERDLETIGAVALDH
                     RVSVGASGLGLGLARALVTSRRVKSNAPDAASGAVVGGAAACLAGSCSQATLQQIANA
                     EAVMPVLHLDADQVVAGKDEARRALAWTKERVKHGPILIASSSTPDQVAALQARHGRD
                     AAGHAIEQAMADIAEGLVEAGVRRLVVAGGETSGAVVDRLGIPGFLVGAEIAAGVPVL
                     RAVGARDGEMLLALKSGNFGGPEFFSDALKLMR"
     gene            complement(9249..10031)
                     /gene="otnI"
                     /locus_tag="VN968_00230"
     CDS             complement(9249..10031)
                     /gene="otnI"
                     /locus_tag="VN968_00230"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HXL27710.1"
                     /translation="MPRFAANLSMMFNEVPFLDRFGAAADAGFTAVEFLFPYDHPPEA
                     IGERLRRNALTQALFNLPPGNWDAGEKGFAALPDRFADLQQSLQTALPYAQATGVKRL
                     HLMAGIANRSDRKAVEAFYKSVAWAAEFFAPHGLDVVIEPINPRNVPGYFLNDFGFAR
                     DLIRELQIPNLKLQFDIYHCQIIHGDVTMRLREMMPITGHVQIASIPSRNEPDGEELN
                     YPFLFSELDLLGYGGFVGCEYNPRGKTTDGLGWFKPYAGAKP"
     gene            complement(10051..10977)
                     /gene="ltnD"
                     /locus_tag="VN968_00235"
     CDS             complement(10051..10977)
                     /gene="ltnD"
                     /locus_tag="VN968_00235"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HXL27711.1"
                     /translation="MPESVGQKPRVAVIGLGSMGFGMATSLKRAGFEVTGYDVSADAV
                     ARFVAEGGSGAKAPAEAAKGADIVVSVVVNAAQTETMLFGKDGVAETLAKGAVFVSSA
                     TMDPDVARRLAKQLEATGRHYLDAPISGGAQRAAQGELTILASGSPAAFAKARPALDA
                     MAAKLYELGDAAGQGAAFKMINQLLAGVHIAAASEAITFAARQGLDIRKVYEVITASA
                     GNSWMFENRVPHVLDGDYTPRSAVEIFVKDLGIIQDMARTAKFPVPVASAALQMFLMT
                     AASGMGHDDDASVARMYARVTDTRLPGEPKKK"
     gene            11095..>11246
                     /locus_tag="VN968_00240"
     CDS             11095..>11246
                     /locus_tag="VN968_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXL27712.1"
                     /translation="MTETGLREEICRLGRSLFERGLTPGSSGNISAKLDDGGWLVTPT
                     NASLGSL"
BASE COUNT         2079 a         3589 c         3547 g         2031 t
ORIGIN      
        1 ccgcaacaga tcgatctcgg tttccgacga gcgacggcca atcatcgcgc gctcgatgga
       61 atgctcaggg ccgctgcgaa accgctgcat catgccgcag cacatcacgc cccagcgcag
      121 cgtgcccatc acttcccaga actttacccg ctcggggtca acacggcggc cggccgcctc
      181 atatccggcg aacagctcgt cgcgggtgcc aaagccgccg accggcttgt cgatctcgcc
      241 aaagcgccag gaattgacgc agacccaccc gagatcctcc atgggatcac cggtatgcgc
      301 caactcccag tcgagcaccg cgcgcaaccc gtcgggaccg ataatgagat tgccgttgcg
      361 gaaatcgccg tgcaccagcg ttacctcttg cgacggaccg ggatcgcggt cgcgcagcca
      421 gcgcagcgcc aattcaaaca ccggccgcgg ccaatcgaaa ccgcggtagt cgcggtcgag
      481 ctcggtaatt tctttcgctg atgtcatgcg gcgaagtttt ggcagtttcg ccagcggcag
      541 cccatggatg ccggccgcga ccttgccgag ttggcgcgcc aggtgcggac gggcgcttgc
      601 gaacttctcg tcgcgcagaa tcttgcgcgc gatggtttcg ccttccacgc gctgcatgat
      661 gaagccagtg ccgagcccgt cctgcggctg cagcacatgt agcacgcgcg gcgacggcag
      721 gccggcctca tgtgcaagct gcatcagcac cgcctcggca ttgagcccgg cggcccgcga
      781 aggtgccgca ccatggcccg gcggcgcgcg gcgcaggatc gcgccgaaga ccccgtcggg
      841 atgcaggata tcgaacgacc aggtgtcctg gctggcgccg ccggagagtt ttgcagcacc
      901 gatgacgccg gtcgcacccg gacaccacga ggcgacgcag cgtccgagtt gctgttcgat
      961 catttgccct tgaactttgc cggacgtttt tcgaggaagg cggtgacgcc ttctctaaag
     1021 tcttccgccg cgccggccct gcgctgcatc tgctgttcga gatcgagctg atcctcgaac
     1081 gagttgtcag aactctccca gtacaatctt cggatcaacg acagcgcaat ggtcggtcct
     1141 tcagccagtt cacgcgcgag cttcatggtc tcctccatca gcgtcgtgtc gtcatagacg
     1201 cgattgacca gaccccattc gagcgccttc tccgccggaa gccgttcgcc catcagcgac
     1261 agttcgaccg agcgtgcctt gccgatcagg cgcggcaaca gccaggtcga cccgcaatcc
     1321 ggcaccaggc cgatgcggcg gaaggcttgc aggaaataag ccgagcgcgc acacaggatc
     1381 atgtcgccca tcagcgcaaa actcatgccg gcgcctgccg cggcgccgtt gaccgaggtg
     1441 acgatcggac aatgcagatt gcgcaggcgg cgcaggaaag ggtgatatcc ggtttcgagg
     1501 gttacgccgg cattgcttcg gctgcccggc ttctgattgt cacgcccctg cagattggcg
     1561 ccggcgcaga acgcgcgtcc cgcgccggtc atgacgagac agcgtacttc gttgcggcgg
     1621 tcgtcaatcg cgtcgagcgc ctcaccgagg ccgcccagca tgtccatgga aaccgcgttc
     1681 atgacttccg gatggtcgag cttcaggatc gccagcgcgc cgtcgaaatc gagcgtaacg
     1741 tgcttgaact gcatctgtgt ttcctcgtcg gttttggccc gcgttatctc tcgcggcgca
     1801 agggttcaat ctcgaagcca tatttgattt ttaagggcag cttgtccatg gttggtatcg
     1861 gaccatctgc ccggcagcaa atgcgcggtc tggagcattt tcagtcccgt ggcttcatcc
     1921 ttcgagacgc ggcgaagacg ccgctcctca ggatgaggtc ttagaccctc atggtgagga
     1981 gcgcggcaac gcagcgcgtc tcgaaccacg aggccaagcg agacgcggtc attaatcaac
     2041 caaagcggaa tgcgccctaa cgggtctcgc cttcggcgag cccgatgaca agctccagcg
     2101 gggtatccag tacgcctcga ttccactatc cgtctctgga tcgcccgttt gcgcaggcga
     2161 tgacggtggt gagtgtggcg cgtgttgagc ccgtcattgc gagcgcaagc gaagcaatcc
     2221 atgttgctgc gaagtaagtg tggattgctt cgtcgctttg ctcctcgcaa tgacgtcaag
     2281 actccgcatc cctgcgacac gactgatttg cagtccggca aatcagccgg cctgccacca
     2341 ccgttcgaag gcggacgcct agccctcttt atcgccggtg agcggcgggt taggtttccg
     2401 ggaatccgtt agaacaggga atgcacaatg gaatatgtcc ccgacgacct cacgccacgc
     2461 gagcgctaca aggtgctgac gtcatttgtc atgccgcggc cgatcgcttg ggtaacgtcg
     2521 gtcggcccta ccggggtcgt caacgctgca cccttcagtt tcttcaacgt attttgcgag
     2581 gatccgccgc tgtgcatgtt cgccgtcaac ctgcggcccg acggccgggt caaggacacg
     2641 ctggtcaaca tccagcgcag cggagagttc gtggtcaacc tcaccgacga accattggcg
     2701 cgcgcgatgc acgactccag cggcgacttt ccgcccgata tcggcgagcc cggctacctc
     2761 gggctgaagc tggcaccctc gaccaaggtg gcagtgccgc ggcttgccga cacgccgtgg
     2821 gcaatggagt gcaagacgtg gaagacgatc gatgtgaacg gcaaccggca actcatcatg
     2881 ggtgaaggca tcaatttcta catccgcgac gagctatggg ataatgaggc catgcgcgtg
     2941 cacatggacc gctaccatcc ggtcggccgc atgttcgcgg accgttactg ccgcaccgac
     3001 gaccgggtcg aattcccggc agcccagggc gcatagctgg ataagactgt agcgatcagg
     3061 aagccgctat cttctgcggc tgctccacag ccgtcatgac gaaacctttg taacccttcg
     3121 ccgcgacgtt attgcagatc tggcggtaga cgccgacgcc accgatgtag ggcatgaaga
     3181 tctgcggctt gcccgggatg ttggcgccca tgtaccagga attggcctgc ggatagagcg
     3241 tggtatgcgc gacctcgttg acatgcgcga cccagctctc ctccgcctgc ttctccgctt
     3301 ccatcgcctc aaggccgcgg tcgcgcatat gggcgatgca atcggcgatc cagtcgacgt
     3361 gctgctcgat cgacaccatc atattgctca gcaccgacgg gctgcccggt ccggtgatga
     3421 tgaacaggtt tggaaaaccc gcgctcatca cgccgagata ggttttgggc ccctcagccc
     3481 atttctggtt cagcgtctgc ccgttgcggc cgcggatgtc gatcttggcg actgacccgg
     3541 tcatcgcatc aaaaccggtc gccagcacca atgcgtcgac ctcgtaatcc ctggcagcgg
     3601 tgcgcaccgc gttcggcaat atctcctcga tcggattcga cctgatatcg accagtgtca
     3661 cgttcggacg attgaaggtg gcaaaataat cggtatcgac gcaaatccgc ttcgtcccga
     3721 tcgggtgatt gttgggctgc agcagttttg ccgtttgcgg gtctttgacg atctccgcga
     3781 tcttttcgcg gacgaaatcg gaggcggtgt cgttggccgc cttgttgagc gcgagatcat
     3841 tgtaaaccgc catgaaggtg aggccgccat aggcccagcg cgattcgtat ctcgtgcggc
     3901 gctcattgtc gccgtcatca agcgcgccgc gatcgggcat ctcggtatag atgccgttgc
     3961 gcgtttcttc acgcgcgaac cgcctcactt ccggatattt tgcgcgaaac gcctcgcgct
     4021 cctcctgact cagcggcgca ttgtgagcgg gaatggaaaa attcgcggtg cgctgaaaca
     4081 ccgtgaggtg gctggcctgc tcggcgatca ccggcaccga ttgaatcccc gaggagccgg
     4141 tgccgatcac ggcaacgcgg aggcctgtga attccaccgg ttcatgcggc cagtggccgg
     4201 tatgatagac cttgcccttg aagccggcga gtccctttat gtcgggcatc cgcgcattcg
     4261 acaggcagcc ggttgcgagc acgacgtatt ttgccgtcac ggtgttgccg tcggatgtgg
     4321 tgacccgcca cgtgttggcg gtttcgtcga aaatcgcctg atcgatgcgg atgttgagcg
     4381 caatatcggg ccgcagcttg aagcggtcgg caacgtggtt ggcgtatttg aggatttcgg
     4441 gctgcggcgc gtagcgctcg ctccagtccc attcctgctg cagtttgtcg gaaaacgaat
     4501 aggaatactg catgctttcg acatcgcagc gcgcgccggg atacctgttc cagtaccagg
     4561 tgccgcctac gccatcgccc tgctcataga cccggaccgt tagtcccagc ccgcgcaatt
     4621 tgtgcagcat gtacatgccc gcaaagcctg cgccgaccac gacgacatcg taggtttcgc
     4681 cgccagcggc gtggtctttg ccggaaattg gttgcaaggc ggacattcga cctccctcgg
     4741 atttttcttg cgttgcggac agcattctac ccacgggcgt gaaagcgcaa gagacaaaaa
     4801 agccccgccg gcttgcatat tttgccacac ggaatgctcg accctcatcc tgaggagcgc
     4861 gcgcttgcgc cgtctcgaac catgaggccc cggtcgggcc tgcatccgtc gagacgcgcg
     4921 caagtgcgcg ctcctcagga tgaggcgcat ttcgttgcac cgcttggcag ggacgcccat
     4981 gatgggctag cgtcgtcaga gaaatcgcgg aaattcaaac aaagtacgag cggcataaaa
     5041 ggccgccgca gggagtgaac caaccatgaa atcgccaatc tgcgacatgc tgggaattga
     5101 gtttcccctg ctggcgttca gccattgccg cgacgtggtt gcggcggtga gccgcgccgg
     5161 cggtttcggc gtgttagggg ccacctccca tacgcctgaa accatcgagc aggaactgaa
     5221 atggatcgac gatcacatcg acggcaagcc ctatgggctc gacgtgctga tcccggaaaa
     5281 tatttcgacc gcaggcgaga aggacgtcac ctggaaaagc ctggaggcgc ggatttccaa
     5341 cgaacatcgc gccttcacca aaaccctgct gaagaaatac ggcatcgagc tgacgacgac
     5401 cagcgtcgcc gacaaccggc cgcagccgtt cgatgggcaa tgcgcgctgg aagtgctcga
     5461 tgcctcgttc aagcatccga tccggctgat tgcgaacgcg ctgggagtgc cgccgaaggc
     5521 gatgatcgac aagggcagaa agcacggcgt gccggtcgcg gctttggtcg gcgccaagga
     5581 gcatgcgctg cgtcaggtcg cggccggcgt cgatatcctg gtggtgcagg gcaccgaggc
     5641 cggcggccat tgcggcgagg tctcgaccat ggtgctggtg ccggatgtga tcaaggcgat
     5701 caagcccatt cgcgacgtgc cggtgctggc ggcgggcggc atcatgaccg ggcggcagat
     5761 ggcggcgtgc atggcgatgg gcgccgccgg cgtctggacc ggctcggtgt ggctggcaac
     5821 ggtcgaatcc gagaccacag aaattttccg cgaaaagatg atcgcggcct cctcgcgcga
     5881 cgccgtccgc tcgaagggcc gtaccggaaa gccggcgagg caattgcgtt cggtctggac
     5941 cgatgcctgg gatcgcggat ccgacagccc cggcgcgctg ccgatgccat tgcagagcat
     6001 catcagccgg gatgccttca actcgatcga tcgcgccgcc gccgccggca acgcgcaggc
     6061 gcgcgacctc gtcagctatt ttgtgggcca aggggtcggc ctgatcgaca gcgtgaaatc
     6121 cgccggcgcc gtggtgcagg aattcaagga agattttgcc gatgcggtcg agcatatgaa
     6181 cgcgctggtg agggaatagg cgcgcgaggc acatccttcg aggctcgccg caagagcggc
     6241 gagcacctca ggatgacggg tcgtgcgtgg ccgtcaccct gaggtgccgt agcgaagcga
     6301 agacctcgaa ggggcgacgg cgaaattgat ggtctggaaa gtgaaataag aaaatgcctg
     6361 ccaactttgt tcccgacgac cgcgttcccg tcatcgtcgg cgttggcgaa attgtcgatc
     6421 gcccgaagga gattcttgaa ggcctcgagc cgctcgcctt gctggagcag gcgctcaagc
     6481 gtgccgaagc cgacagtggc ggcaaattgc tcggcgaaat cggttcgctc gatatcgtca
     6541 atttcctgag ctggcgttac cgcgatccgg aaattctgct ttccgaccgt ctcggcatca
     6601 agcccaagca tgcctattac ggcccggtcg gcggcgagag cccgatccgc tatctgcatg
     6661 aggccgcgca acgcatcgcg cgcggcgact gcagcgtcgc cgcggtctgc ggcgccgaag
     6721 cgcaatccac cgcgaccaag gccgaacgcg ccggcgtcga actgccgtgg acgccgttcg
     6781 cacacgatgt gcccgagccc aagcgcggcg ccgcgttcca gaagccgatg gcggtaaaac
     6841 tcggcgtatt ccggcccatt acggtgtatc cattttacga agcggcgacg gcggcgcatt
     6901 ggggccagac cccgcgcgaa gcgatggcgg aatcaggcgc gctgtggtcg acctatgcag
     6961 acgtcgcgtc acaaaatccc aatgcctggc tgaggcggcg ctttacgtcg gatgagatca
     7021 cgaccccgac gcttgaaaac cggctgatcg cctggcccta caccaaactg caggtggcca
     7081 atccaaccgt caacatgggc gcggcggtgc tgatgacgag ccttgcaaag gcgcgagcgg
     7141 cgggcatcgc cgaggatcgg cttgttcacg tctggggcgg agcttcggcg gaagagccac
     7201 gggactatct gatccgcgac cagtttttcg agagccatcc gcagaacgcc gtgctcaacg
     7261 cggtcctgga tctcgtcggc ggcaatggca aggcgttcga cgcaatcgag ctctacagct
     7321 gctttccctg cgtgccgaag atggcgcggc ggacgctcgg gctcggcact gaggtgcagc
     7381 caaccgtgac cggcggcctg acgtttttcg gcgcgccact caacacctac atgacgcacg
     7441 cggcctgcgc gatggtgcgc aagctccgca gcggcgcaaa tttcggcctg ctctacgggc
     7501 aaggcggatt cgtgacaaaa catcacgcgc tggtgctgtc gcggcaggcg ccacgcggcg
     7561 cgctggcgca ggacaccagc gtacaaacag aggcggaccg ccatcgccgg gcggtgccgg
     7621 agttcgtcac cgaggccaat ggcaagggcg aggtggagag ctttaccgtg atctatggcc
     7681 gcaacggcga ggccgagcac gacgtggtga tgctgcggac gcaagacaag gcgcgcgcgc
     7741 tggcgcgggt gcccgccagc gacagggcgg cgctttccca tcttctcgac ctggaccgca
     7801 gcccggtcgg ctcgcgcggc gacatcgtca cagcggacga cggcgtgctg gagtggcggg
     7861 tggggtgaca tcggcgctct ctcatctccc tcgccccgct cttgcggggc gaggtgaaaa
     7921 gcagtgcgcc tcgctcgcat atcccgttcg cgtccgcgat tccgtgcaat tcgctcagcg
     7981 catcagcttg agcgcatcgg agaaaaattc cggaccgcca aaattgccgg acttcagcgc
     8041 cagcagcatc tcgccgtccc tggcgcccac ggcacgcagc accggaacgc cggccgcgat
     8101 ttcggccccg accagaaagc cgggaatacc cagccggtcc accaccgcgc cggaggtttc
     8161 gcccccggcg accaccagcc gccgcacacc ggcctcgacc agcccctcgg cgatgtcggc
     8221 catcgcctgc tcgatcgcgt ggcccgcggc gtcgcggccg tggcgcgcct gcagggccgc
     8281 gacctggtcc ggcgtcgaac tagaggcgat caggatcggg ccgtgcttga cccgctcctt
     8341 ggtccaggcg agcgcgcgcc gcgcttcatc cttgcccgcg acgacctggt ccgcatcgag
     8401 atgcagcacc ggcatgacag cttccgcgtt tgcgatctgc tgcagcgtcg cctgcgagca
     8461 actgccggcg agacacgccg ccgctccgcc gaccaccgcg ccggaagccg catccggtgc
     8521 attcgacttg acccggcgcg aagtaaccag cgcgcgggca agaccaagcc cgaggccgga
     8581 tgcgccgacg gagacgcgat gatccagcgc cacggcgccg atggtctcga gatcgcgttc
     8641 gaacaccgcg tccgcgatcg ctgcgccaaa acccttgccg gagagatcgg caagccgggc
     8701 gcgcacggca tcgggcccgc gcgcgatgtc ggccagatcg acaagcccga cctttgcttt
     8761 gctctggcgc gccagcaccc gcaccagatt ggaatcatgc atcggattga gcggatggtc
     8821 ctttaagggg ctttcgttca gcggcacggc gccgacgaac agatttccct gatacaccgt
     8881 gcggccggtc tccggaaatg ccggcgtcac cagcacgatg gcatcgccgg aatcggcgcg
     8941 cagcgcatcc atcaccgggc cgatattgcc ggcgtcggtg gaatcgaagg tcgagcagat
     9001 cttgaacaac acatgatcgg caccccgctt gcgcagccat ttttccgcat cccgcgagcg
     9061 cgacacggca aggcttgcct cgatcgagcg gctcttgagc gacaccacca ccgcatcgac
     9121 gtcgggaagc gcgagatcgc ctgcgggcac gccgatggtc tgcaccgtgc gcaggccgca
     9181 gcgggtcagc gtgttggcga ggtcggacgc gccggtgtaa tcgtcggcga tgcagccgag
     9241 cgccagcgtc acggttttgc tccggcatag ggcttgaacc agccgagacc gtcggtggtt
     9301 ttgccgcgcg ggttatattc gcagccgacg aatccgccgt agccgagcag gtcgagttca
     9361 ctgaacagga acggatagtt caattcctcg ccgtccggtt cgttgcgcga ggggatgctg
     9421 gcgatctgga catggcctgt tatcggcatc atctcgcgca gccgcatggt gacgtcgcca
     9481 tgaatgatct ggcaatgata gatgtcgaac tgaagtttca gattggggat ttgaagttcg
     9541 cggatcaggt cgcgcgcgaa gccgaaatca ttgaggaagt agccgggcac gttgcgcggg
     9601 ttgatcggct cgatcacgac gtcgaggccg tggggcgcga aaaattccgc tgcccatgcc
     9661 accgatttgt aaaacgcctc caccgccttc cgatcgctgc ggttggcaat gcccgccatc
     9721 agatgcagac gcttgacgcc ggtcgcctgc gcgtaaggca gcgcggtttg aagactctgc
     9781 tgcagatcgg caaaacggtc cggcaatgcc gcaaagccct tttcgccggc gtcccaattg
     9841 cccggcggca ggttgaacag cgcctgcgtc agggcgttgc ggcgcagccg ctcgccgatg
     9901 gcttccggcg gatgatcgta gggaaacagg aattccacgg ccgtgaatcc cgcatctgcc
     9961 gccgcgccaa aacggtcgag gaacggcact tcgttgaaca tcatcgagag attggcggcg
    10021 aaacgcggca tctgctgtcc tcttgctcat tcacttcttt ttcggttcgc cgggcagacg
    10081 cgtatcggtg acgcgggcat acatccgcgc caccgaggca tcgtcatcgt ggcccatgcc
    10141 ggaagccgcc gtcatcagaa acatctgcag tgcggcggat gccaccggca ccggaaactt
    10201 tgcggtgcgc gccatgtcct ggatgatgcc gaggtctttg acgaaaatct ccaccgcgct
    10261 gcgcggggtg tagtcgccgt cgagcacatg cggcacgcgg ttctcgaaca tccaggaatt
    10321 gccggcggac gccgtgatca cctcgtagac tttccttata tcgagccctt gcctggccgc
    10381 gaaggtgatc gcttccgaag cggccgcgat atgcacgccg gccagcaact ggttgatcat
    10441 cttgaaggcg gcgccctgcc ctgcggcatc gccgagttca tagagttttg ccgccatcgc
    10501 gtccagcgcg ggcctcgcct tggcgaacgc cgcgggactg cccgatgcaa gaatcgtgag
    10561 ctcgccctgc gctgcgcgct gcgcgccgcc agagatcggc gcgtcgagat agtgccggcc
    10621 ggtcgcctcc agctgtttcg ccagccgccg cgcgacatcg gggtccatgg tggcggaaga
    10681 aacgaacacc gcgcccttcg ccagggtctc ggcaacgccg tccttgccga acagcatggt
    10741 ttcggtctgc gcggcattga cgaccacgct gacgacaatg tccgcgccct ttgcagcctc
    10801 ggccggcgcc tttgcgccgc ttccgccttc agcgacgaac cgggcgacgg cgtcggccga
    10861 aacatcgtag ccggtgactt caaaacccgc gcgcttgagc gaggtcgcca tgccaaaacc
    10921 catcgagcca agcccgatga cggcgacgcg cggtttttgg ccaacggatt caggcatgcg
    10981 gcaattcctt tgaggttcct cttttggccg acatttgaca gcggcttcgc cctcggtatc
    11041 acggcttggc cgcgctgcca aagcatgaga caaggcggca aagagggtgc tgccatgacc
    11101 gaaaccgggt tacgcgagga aatctgccgc ctcggccgtt cgctgttcga gcgcgggctg
    11161 acgccgggct cgtcaggcaa tatcagcgca aaactcgacg acggcggctg gctggtgacg
    11221 ccgaccaatg cctcgctcgg gtcgct
//
LOCUS       DATNOI010000008        12863 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1782140,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000008 DATNOI010000000
VERSION     DATNOI010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12863
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782140"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VN968_00245"
     CDS             complement(<1..435)
                     /locus_tag="VN968_00245"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXL27713.1"
                     /translation="MTKFRHLIWMMIAAAGLTLSVSQSFAQQPDVGDQPGLLPDDSVE
                     LDPQYRRTAVLYRTTEAPGTIIISTAERHLYLIQGNGRALRYGIGVGREGFQWQGLLN
                     ISRKAEWPDWTPPPEMIARQPYLPRFMAGGPGNPLGARAMYLG"
     gene            complement(526..657)
                     /locus_tag="VN968_00250"
     CDS             complement(526..657)
                     /locus_tag="VN968_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2474 domain-containing protein"
                     /protein_id="HXL27714.1"
                     /translation="MKPSGQHPRPLAQRLLWFAALWLGGVGTVAAVAFCLRLWIAPK"
     gene            complement(654..1667)
                     /gene="cydB"
                     /locus_tag="VN968_00255"
     CDS             complement(654..1667)
                     /gene="cydB"
                     /locus_tag="VN968_00255"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HXL27715.1"
                     /translation="MTAFFDIATIWAFIIAFAVFVYVVMDGFDLGLGLLFPLFPNKAD
                     RDVIMNSVAPVWDGNETWLVLGGGGMMAAFPLAYAVLMPALYTPMIAMLIGLIFRGVA
                     FEFRWRTTQSQRNRWDIAFAGGSLLATLAQGIALGAILQGVHVEGRHYAGGWWDWLTP
                     FSILTGLALVVGYALLGATWLVLKTEGELRDKAYHLSWALLFAMLGAIGAVSIATPFL
                     HIHYTQRWFTWPNIILTAPVPIAVAGVAVLLLRSLANKLDTQPFFLSLALFGLSYAGL
                     GISMYPYIVPQSITIWQAASPANSQLFMLVGVAILVPLILGYTGWAYWVFRGKVRSGS
                     GYH"
     gene            complement(1664..3085)
                     /locus_tag="VN968_00260"
     CDS             complement(1664..3085)
                     /locus_tag="VN968_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HXL27716.1"
                     /translation="MFEGWDAVVLARAQFAFTMSFHIVFPAFSIGLASYLAVLEALWL
                     WTGREVFINLFNYWLKIFAIAFGMGVVSGIVMSYQFGTNWSAFSDRAGPVIGPLMAYE
                     VLTAFFLEAGFLGVMLFGLERVGHRLHFFATLMVATGTLISAFWILSANSWMQTPAGY
                     AVNADGQFVAADWLKVIFNPSFPYRLVHMVLAAYLTTALLVGAVGAFHLLRDSHQAGP
                     RVMFSMAMWMATLVAPIQILAGDQHGLNTLEHQPTKIMAMEGHFQSHPDGAPLILFGL
                     PDQAAGEVKYAIEVPMLGSLILKHSIDAPLAGLDTVPRENWPPVPITFWSFRIMVGMG
                     FLMVGLGLFSLWSRWRGTLYQSRLLHMFAMAMGPAGFIAVLAGWITTETGRQPFTVYG
                     LLRTVDSASPLAAPAVASSLIGFVIVYFIVYAAGLTYLFRLMASAPHHGEQGPSADLP
                     ARAAGITPAAGAAAGAATQGSAR"
     gene            complement(3168..3734)
                     /locus_tag="VN968_00265"
     CDS             complement(3168..3734)
                     /locus_tag="VN968_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXL27717.1"
                     /translation="MTIRSLAATAAVVAAYLTSTAIPCLSGELARDRVAPFAVAAVQN
                     AAPNFAGISNWFNSAPLNIADLRGKVVLVDFWTYGCVNCVNTLPHVTDLYAKYRDRGL
                     VVVGVHTPEFPFERSASNVQAALKRHGITYPVAQDNDSQTWNAYSNRYWPAQYIVDQS
                     GKIVFQHDGEGQYEQMDRIIARLLNASS"
     gene            complement(3763..4461)
                     /locus_tag="VN968_00270"
     CDS             complement(3763..4461)
                     /locus_tag="VN968_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HXL27718.1"
                     /translation="MFNLVLALLAGVVTIAAPCTLPMLPILLGASVGQAGKARPALIA
                     LGFVISFSAAALLLSAITRVFDFDPNVLRTGAAIVLLGFGVLMIWPAPFERFSALVGG
                     LTNGGPGDSAFRQGNIGGFVLGSTLGLVWTPCAGPVLGSILTIVATSRDTAWASALLV
                     VYAVGAAIPMLAIAYGGQAVTTRVRSIARISPRLQQGFGVVVIAFAVASYFQYDTLIV
                     AWLTGFYPSGQIGL"
     gene            complement(4993..5604)
                     /locus_tag="VN968_00275"
     CDS             complement(4993..5604)
                     /locus_tag="VN968_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXL27719.1"
                     /translation="MQNVVAINSAAREGIIAAQATSDEMLLESIAEGGRTAMHTLYSR
                     HNVRVYRFVLRIVRDATMAEDLVSQVFLDVWRTASQFEGRSQVSTWLLSIARFKALTA
                     MRQRRHEDIDQEDVLEIADQADTPEASLNRSNTSEILRACVAKLSPAHREIINLVYYH
                     EMSVEEAGAIIGIPQSTVKTRMFYARKQLADLLKGAGVEGLAA"
     gene            complement(5923..6666)
                     /locus_tag="VN968_00280"
     CDS             complement(5923..6666)
                     /locus_tag="VN968_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL27720.1"
                     /translation="MTQSPHIIIVDDEAPAREMVGEYLKMHGFTVTLCDGGRSLRGAI
                     ETSVPDLVVLDLNMPEEDGLSIIRDLKSRTNVPVIMLTATASPIDRVVGLELGADDYI
                     AKPCELRELMARIRSVLRRSSPAKAPAATPETAAAKAAKDQLVRFGTKWLDLEAQALR
                     DDEGNEHPLTASEFGLLKVFAANPKRVLSRERLLELANARDAEAFDRAVDLRIMRIRR
                     KIEMDPTKPAVIRTIRGGGYLFSPAGDKA"
     gene            complement(6663..8408)
                     /locus_tag="VN968_00285"
     CDS             complement(6663..8408)
                     /locus_tag="VN968_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HXL27721.1"
                     /translation="MAEQDDVLHLIDDSGVAPEASTTRRWKVAVIDDDQAVHEGTRFA
                     LSDYNLNGQKLEILSAYSAAEGRTLMRAHPDIAAVLLDVIMETDAAGLDLVEYIRNEI
                     KNETVRIILRTGQPGQAPERRVIVQYDINDYKAKTELTADKLFTSLTAALRSYQQLER
                     MVQTRRGLEIIIDAASTLYDFRSMQRLAEGVLTQIASLLNVDCAGILVLRDGGVVGDD
                     FSVLAGSGCYSRFIGSAGSKSLDPDLRSMVEAAFKRRKHEFVDHRTVLYVRTGSGREV
                     VVLLQAERPLSETDRSLVEIFGSRLSIAFDNVILYQQLHEANTQLEDRVAQRTRALMQ
                     ANRRLSAQWLRLQRANGFKNEILGTVAHDLKNPLGVILGRTEMLTELIGAGSSKESVT
                     AQVEHIRDATRRLTSMVDHLISDAMADAFDITIRREPVDIAGLVSEVADANQPLAVNK
                     QQTIAVSAPPNFVTMCDADRMREAIDNLVSNAIKYSPIGGKIAVVVTHEQNTTIIRIA
                     DEGAGLSPEDLGRLFGRFQRLSAKPTAGESSTGLGLSIVKRIIDMHGGQVTANSEGPG
                     QGSTFTVILPATEIS"
     gene            complement(8378..10363)
                     /locus_tag="VN968_00290"
     CDS             complement(8378..10363)
                     /locus_tag="VN968_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL27722.1"
                     /translation="MPIRWRILSIAALNSAVVVVLSAMIWNGAQVLGSAWDDVRQARE
                     SDKILAMLESETGRLQNLIHRYINQSSPELFAEILLLREAVLGTLTTRASTDPMLSGS
                     VEELERVTERFLNGFGELRGVQATISKTYDEQVLTPARDMAGLYSIIEGATGHRDALI
                     WPSLGKSREAFTAMLVAANAYYLSLATASAEEARRNTDTIEKTIPVMSDLADNDLQRM
                     ALQRLNARTVALREGLAKLSEQLASRTELLRNTIDASQADAIGAIDRLSVKMRQREQK
                     AQETFDRTLADISRRVLSIAVIFLGIILTAGVLIALSIRLPLGQIMTAMHAITSGDYD
                     RRVQGTDARDEVGAMARAVEVFRENAIAKRKTEDELRASKEKAEGALFELNAAQQNLI
                     DAERLAALGGLVAGVAHEVNNPIGISLTVASSFARRAEMFEAELRSDGPLRRSQLEEF
                     VRNSRDAAQQLVANLHRAGELIQSFKQVAVDRSHAERRQFSLGEATDQIIASLRPVLK
                     KAAIALSVDVPEGLLIDGYPGSYGQILTNLFLNAANHAFPDGRSGKISISARARGVDD
                     VEIIFADNGAGMTPDVQRQAFDPFFTTRRNEGGTGLGLHIVYNLVTQQLGGRMMLDSR
                     LGQGTTFRIIMPRAAKGGSTEASDGTPQWPNRTMSST"
     gene            complement(10463..11458)
                     /locus_tag="VN968_00295"
     CDS             complement(10463..11458)
                     /locus_tag="VN968_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXL27723.1"
                     /translation="MPVYSRADLSRTIAPVVALLLAAVWTSAVGREFRAADSQNEDYP
                     TVQALRYMGRMVTERSGGRHQIRVFHSRQLGEEKETIEQTRAGAIDLNRTNGALIGNF
                     VPAMNVLAMPFLFRSIEHLQKVLDGPIGNEILNSFEPYGFVGLAFYDSGARSIYNSVR
                     PVRSIADIRGLRLRVQQSELMSDTIKALGAEPIELPYGQVLTALSTALIDGAENNWPS
                     FVTSGHYKYAGYYTLTEHAMSPEVLVMSQKAWESLSAEDRTIFRDAAIRSSHFMHDKW
                     KDLEQQSRRQAEAEGVTIVTDFDRKPFEAATAGIYQKAQRDPAVAELIERIRKVE"
     gene            11628..>12863
                     /gene="ugpB"
                     /locus_tag="VN968_00300"
     CDS             11628..>12863
                     /gene="ugpB"
                     /locus_tag="VN968_00300"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HXL27724.1"
                     /translation="MKTVSTAADRKVLIVKSTLRFLQLAAVVAAVTLASPALAVTDIM
                     WWHAMSGELGRQLDKLAANFNASQSEYRIVPSYKGNYTETVTAAIFAFRSRSQPAIVQ
                     VSEIATATMMAARGAIYPVFELMRDEHEAFSTAAYLPAITGYYADVAGNMLSFPFNSS
                     TPILYYNKNLFRAAGLDPEVAPKTWSEVGAAAKRLRAYGAVCGFTTSWPSWINVENFS
                     AFHNLPMSTKANGFDGLDAVLTFNNPLMERHIAQLAEWQTSKMFDYSGRATSAEPRFQ
                     NGECGIFIGSSATRADIRANSKFEVGYGMLPYWPDVEGAPQNTTIGGATLWVLRDRPR
                     EEYKGVAKFFGYLSKPEIQAAWHQNTGYLPITRAAFELTRAQGFYDRNPGTAISIEQI
                     TLKTPTENSRGVRLGSFVLI"
BASE COUNT         2467 a         3988 c         4078 g         2330 t
ORIGIN      
        1 gccgagatac atcgcgcggg cgccgagcgg attgcccggg ccgccggcca tgaagcgcgg
       61 cagatagggc tggcgcgcga tcatctccgg cggcggcgtc cagtccggcc attcggcctt
      121 gcgcgaaatg ttcagcaatc cctgccactg aaatccctcg cgtccaacgc cgatgccata
      181 gcgcagcgcg cggccattgc cctggatcag atagagatgg cgctccgcgg tcgagatgat
      241 gatcgtgccg ggcgcctctg tggtgcgata cagcaccgcg gtgcgccggt attgcgggtc
      301 caattccacg gaatcgtcgg gcagaagccc cggctggtcg ccgacatcgg gctgctgtgc
      361 aaagctctgc gacaccgaaa gcgtgaggcc ggccgcggca atcatcatcc agatcaggtg
      421 ccgaaatttc gtcatccaga actctcctcg ttcagcgcaa tgacaattgt ctaccatccc
      481 cggcgaggca tcggaaccac caaaatcgca gccgtcggga gccggtcatt tcggcgcgat
      541 ccacagccgc aggcaaaagg ccacggcagc caccgtgccg acgccgccga gccagagggc
      601 tgcgaaccac aacagccgct gcgccagcgg tcgcggatgt tgcccggatg gcttcaatga
      661 tagccgctcc cggacctgac cttgccgcgg aatacccaat aggcccaccc ggtataaccg
      721 agaatgagcg gcaccaggat ggcgacgccg accagcatga acagctggct gttcgcgggc
      781 gatgccgcct gccagatggt gatgctctgc ggcacgatat agggatacat gctgatgccg
      841 aggccggcat aggacagccc aaacagcgcc agcgacagaa agaacggctg ggtgtcgagt
      901 ttgttggcga ggctgcgcaa cagcaggacg gcaacgccgg ctaccgcgat cggtaccggc
      961 gcggtcaaga tgatgttggg ccaggtgaac cagcgctggg tatagtggat gtggaggaac
     1021 ggggtggcga tgctgaccgc gccgatggcc cccaacatcg cgaacaggag agcccagctc
     1081 aggtgatagg ccttgtcgcg aagttcgccc tcggtcttca gcaccagcca tgtcgccccc
     1141 agcagggcgt agccgaccac cagtgcgagc ccggtcaaga tgctgaatgg cgtcagccag
     1201 tcccaccagc cgccggcata atgccgcccc tcgacatgca cgccctgcag gatggcgccg
     1261 agcgcaatgc cttgcgccag cgtcgccagc agcgatccgc cggcgaatgc gatatcccag
     1321 cgattgcgct ggctctgcgt cgtccgccag cgaaattcga aggcgacgcc gcgaaagatc
     1381 aggccgatca gcatcgcgat catcggcgtg taaagtgccg gcatcagcac cgcataggcc
     1441 agcggaaacg ccgccatcat tccgccgccg ccgagcacca gccaggtttc attgccgtcc
     1501 cacaccggcg cgacgctgtt catgatcacg tcgcggtcgg ccttgttcgg aaacagcgga
     1561 aacagcaggc cgaggccgag atcgaatccg tccatcacca catagacaaa gaccgcgaac
     1621 gcgatgatga acgcccagat ggttgcgatg tcgaagaagg cggtcatcgg gcggacccct
     1681 gcgttgccgc acctgctgcc gcacctgctg ccggcgtgat gccggcggcg cgggccggca
     1741 gatcggcgga cggtccctgt tcgccgtgat gtggcgcaga tgccatcagc cggaacaggt
     1801 aggtcaggcc cgccgcatac acgataaaat agacgatgac gaagccgatc agcgacgagg
     1861 ccaccgcggg ggcggccagc ggcgaggcgg agtcaaccgt cctcaacagg ccgtacacgg
     1921 tgaacggttg gcgcccggtc tcggtggtga tccagccggc gagcacggcg atgaaacccg
     1981 ccggacccat cgccatcgcg aacatgtgca acaaccggga ttggtagagt gtgccgcgcc
     2041 agcggctcca caggctgaac aggccgagcc cgaccatcag aaaacccatt ccgaccatga
     2101 tccggaacga ccagaaggtg atcggcaccg gcggccagtt ctcgcgcggc acggtgtcga
     2161 ggccggcaag cggcgcatcg attgaatgtt tgaggatcag cgatcccagc atcggaactt
     2221 caattgcgta cttgacctcg ccggcggcct gatcaggcag gccgaacagg atcagcggtg
     2281 cgccgtccgg gtgactctgg aaatggccct ccatcgccat aatcttggta ggttgatgct
     2341 ccagcgtgtt gaggccgtgc tggtcgccgg ccagaatctg gatcggcgcc accaaagtgg
     2401 ccatccacat cgccatcgaa aacatcaccc gcggaccggc ctggtgggaa tcgcgcaaca
     2461 ggtgaaacgc gccgaccgcg ccgactagca gcgccgtggt gagataggcc gccagcacca
     2521 tatgcacgag gcggtaggga aacgacggat tgaagatgac cttgagccaa tcggcggcga
     2581 cgaactgccc gtcggcgttg acggcgtagc cggccggcgt ctgcatccag gaattggcgg
     2641 agaggatcca gaatgcggaa atcagcgtgc cggtcgcgac catcaaggtc gcgaagaaat
     2701 gcagcctgtg gccgacccgc tccagcccga acagcatcac gccgagaaaa cccgcttcga
     2761 ggaagaacgc ggtgagcacc tcgtaggcca tcagcgggcc gatcacgggg cccgctctgt
     2821 ccgaaaatgc cgaccagttg gtgccgaact ggtaggacat cacgatgccc gacaccacgc
     2881 ccatgccgaa cgcgatggcg aagatcttca gccaataatt gaacagattg atgaagacct
     2941 cgcgtccggt ccacagccag agcgcttcga gcaccgcgag atagctggca agcccgatcg
     3001 agaacgccgg aaacacgatg tgaaacgaca tcgtgaacgc gaactgcgcc cgcgccaata
     3061 ccacggcatc ccagccctca aacatgtcgg tcatccgccg tcgctattcc ggcgcggacc
     3121 ttaccatgcc gagcgtgacg atggtacagc gccgtgcaca ggaccgctca gcttgaggcg
     3181 ttgagcaggc gcgcgatgat gcgatccatt tgttcgtact ggccctcgcc gtcgtgctgg
     3241 aacacgattt ttccgctttg gtcgacgata tattgcgccg gccaataccg gttgctatag
     3301 gcgttccagg tctgggaatc gttgtcctgt gcgaccggat aggtgatgcc atgccgtttc
     3361 agcgcggcct gcacgttgga tgccgatcgc tcgaacggga attccggcgt gtggacgccg
     3421 acgacgacga ggccgcgatc cctgtatttc gcatagagat cggtcacgtg cggcagcgta
     3481 ttgacgcagt tgacgcagcc ataggtccag aaatccacca gcacgacctt gccacgcagg
     3541 tccgcgatgt tgagcggcgc ggaattgaac cagttgctga tgccggcgaa gttgggtgcg
     3601 gcgttctgta cggcggcgac cgcgaacgga gccacccggt cgcgtgcaag ctcgccgcta
     3661 aggcagggta tggcagtgct cgtcaggtaa gcggcaacca cggcagcggt ggcggcgagg
     3721 gaacgtatcg tcatggcaaa ctccattatc gggcgcggtt gattacaggc cgatctggcc
     3781 gctcgggtaa aatcctgtca gccacgccac gatcagcgtg tcgtattgaa agtaggaggc
     3841 gaccgcgaag gcgatgacga cgacgccaaa tccctgctgc agcctggggg agatccgcgc
     3901 gatgctgcgg acgcgggtgg tgacggcctg gccgccatag gcgatcgcca gcatcgggat
     3961 cgccgcgccc acggcgtaga ccaccagcag cgcgctcgcc catgcggtat ccctggatgt
     4021 cgcgacgatg gtgaggatcg agccgagcac cgggccggcg cagggcgtcc acaccaggcc
     4081 gagcgtgctg ccgagcacga agccgccaat gttgccttgc cggaacgcgc tgtcgccggg
     4141 accgccgttg gtgagcccgc cgaccagggc cgaaaaccgt tcgaacggcg ccggccagat
     4201 catcaaaact ccgaagccga gcaacacgat cgccgcacct gttcgcagaa cgttcggatc
     4261 gaaatcgaag acgcgggtga tggcgctgag caacaatgcg gcggccgaaa acgagatcac
     4321 aaaaccgagc gcgatcagcg ccggccgcgc cttgccggct tggccgaccg acgcccccag
     4381 aaggatcggc agcatcggga gcgtgcaggg tgcggcgatg gtcaccacgc cggcgagcag
     4441 ggcaagaacg aggttgaaca tcagatggga cccatcacag cgctctcatc tgcgccttgc
     4501 ggccgcgtca gagcttgtag ggtccttcga agcggcgagg cgaggcgtta cgcgggcagc
     4561 atttgggccg cgtcacgtgt tcgtgaggct ttcgatgtga agcgcgagcg agttcaatcc
     4621 caacatagat gaatcgccag cgtcatcaga agcggcaagt ggcaataaga acaggacgga
     4681 gaaccgcttc ggcagtccgc aaaagcggag cgacccggag gggggcatcc cgtccgggtc
     4741 gtcggaggtt cttggcggta gaaacctggg aatcagcggg cgcttgtctc gcagtcgtcg
     4801 aaaatcacgc agacctcggc ttgtctgcgg ctctcatgcc cggcgttggt gctcgtcggt
     4861 gtagcagtga agtttttccg gttgatgtct tgaattgttt caattgtttc gcggccggta
     4921 accattgcga acaacaggcg tccggttctt cgcaatcaga tatccactaa caaattgatc
     4981 taattgacat tttcacgccg ccaggccctc gacgccggcg cccttgagca gatcggcaag
     5041 ctgcttgcgg gcatagaaca tccgcgtctt caccgtgctt tggggaatcc cgatgatcgc
     5101 accggcctct tctaccgaca tctcgtggta gtaaaccagg ttgatgatct cgcgatgcgc
     5161 cggcgacagc tttgccacgc aggcgcgcag aatctcgctg gtattgctgc ggttcaacga
     5221 ggcttcgggc gtatcggcct gatcggcgat ttcaagcacg tcctcctggt cgatgtcctc
     5281 gtgccggcgc tgacgcatcg cggtcagcgc cttgaagcgg gcgatcgaca acagccaggt
     5341 ggaaacctgg gaacggccct cgaactggct ggcggtgcgc caaacatcga ggaacacctg
     5401 actgacgagg tcttccgcca tggtggcgtc gcgcacgatg cgaagcacga aacggtaaac
     5461 ccgcacattg tggcgcgagt aaagggtatg catcgcggtc cggccgcctt cggcaatgct
     5521 ctccagcagc atttcgtccg aggtcgcctg ggcggcgatg atgccttcgc gggctgcgga
     5581 gttgatggcg acgacgttct gcatgacagg ctcccaataa tcgccgctgg gcggcgcctc
     5641 gttgggcaaa ttgatagtca gcgaacgttt cgggacgtct gcacgcaaag gggaaaatgg
     5701 tttcatgtgc cggagaattg tttcgtcgct acggctgcga cgaaacattc gagctcgaaa
     5761 tgtgagcaac ttcaatgatc gcaaatctgg aagatttgca gacgcaaaaa agttcctgtc
     5821 ctcgcatgaa aaaattgggt tcactgcgga cggattggcc cctttttgcc tctcccggcc
     5881 tgcggaaaga gggggcaatt cacggttcgc tcgggattcc gatcaggcct tgtcgccggc
     5941 gggcgagaat agatagccgc cgcccctgat ggtgcggatc accgcgggct tggtcgggtc
     6001 catctcgatc ttgcgccgga tccgcatgat ccgcagatcc acggcgcgat cgaaggcttc
     6061 ggcgtcgcgc gcgttggcca actccagcag gcgctcgcgc gacagcaccc gcttcggatt
     6121 tgccgcgaaa accttgagca ggccgaattc ggaagcggtc agcggatgct cattgccctc
     6181 gtcgtcgcgc agcgcctgcg cctccagatc gagccatttg gtgccgaacc gcaccaattg
     6241 gtctttcgcc gccttcgcag ccgcggtttc cggcgttgcc gctggcgcct tggccggcga
     6301 gctgcgccgc aataccgagc gaatccgcgc catcaattca cgcaattcac agggctttgc
     6361 gatgtaatcg tcggcgccga gttccagccc gacgacgcgg tcgatcgggc ttgcggtggc
     6421 cgtcaacatg atgaccggga cgttggtccg gcttttcaga tcgcggatga tggaaagccc
     6481 gtcctcctcc ggcatgttca gatcgagtac caccagatcg ggcacactgg tttcgatcgc
     6541 gccgcgcagg ctcctgccgc catcgcacag cgtcacggta aaaccgtgca tcttgagata
     6601 ctcgccgacc atctcgcgcg ccggcgcctc gtcgtcgaca atgatgatgt gggggctttg
     6661 ggtcatgaaa tctctgttgc aggcaagatg acggtgaagg tcgagccctg tccgggcccc
     6721 tcgctgttcg cggtgacctg gccgccatgc atatcgatga tgcgcttgac gatcgaaagc
     6781 ccgaggccgg tcgaactttc gccggcggtg ggttttgcgg acagccgctg gaaacggccg
     6841 aacaatcggc cgagatcttc gggggacagg ccggcgcctt cgtcggcgat gcggatgatg
     6901 gtggtgttct gctcgtgcgt taccaccacc gcgatcttgc cgccgattgg actatatttg
     6961 atggcgttgc tgacgaggtt gtcgatcgcc tcccgcatcc ggtcggcatc gcacatcgtg
     7021 acgaaattcg gcggcgccga caccgcgatg gtctgctgct tgttcacggc caagggctgg
     7081 ttggcatcgg cgacttcgct gacaaggcct gcgatatcca ccggttcgcg gcgaatggtg
     7141 atgtcgaagg cgtccgccat cgcgtccgaa atcagatgat cgaccatcga ggtcaggcgc
     7201 ctggtggcat cgcggatgtg ctcgacctgg gcggtgacac tctccttgga agagccggcg
     7261 ccgatcagct ccgtcagcat ttcggtgcgg ccgaggatga cgccgagcgg attcttcaaa
     7321 tcgtgagcca cggtgccgag aatttcattc ttgaaaccgt tggcgcgctg cagccgcagc
     7381 cattgcgccg acagcctgcg gttggcctgc atcagcgcac gggtgcgctg ggcgacacgg
     7441 tcctcgagct gcgtgttggc ctcgtgcagc tgctgataaa gaatgacatt gtcgaacgcg
     7501 atcgaaagcc ggctgccgaa tatttcgacc agcgaccggt cggtctcgga tagcggacgc
     7561 tccgcctgca gcagcaccac cacttcccgg ccgctgccgg tgcgaacgta gagaacggtt
     7621 cggtgatcga cgaattcatg tttgcgccgc ttgaaggcgg cttcaaccat cgaccgcagg
     7681 tcggggtcga gcgacttgga tccggcgctg ccgatgaagc ggctatagca gcccgaaccg
     7741 gccagcacgg aaaagtcgtc gccgaccacg ccgccgtcgc gcagcaccag gatgccggcg
     7801 caatcgacgt tcagcaggga agcgatctgc gtcagcacgc cctcggcgag gcgctgcatc
     7861 gatctgaaat cgtacagggt cgaggcggca tcgatgatga tttccagccc gcgtcgggtt
     7921 tgaaccatgc gttcgagctg ctgatagctg cgcaacgccg cggtcagcga ggtaaacagc
     7981 ttgtcggccg tcagctcggt cttcgccttg tagtcgttga tgtcgtactg cacgatgacg
     8041 cggcgctcgg gcgcctggcc gggctgcccg gtgcgcagga tgatgcgaac ggtctcgttc
     8101 ttgatttcat tgcggatgta ttcgacgaga tcgaggcccg ccgcgtcggt ttccatgatg
     8161 acgtcgagca gcaccgcggc gatatcggga tgcgcgcgca tcaatgtgcg gccctcggcc
     8221 gccgaatagg ccgagaggat ttcgagcttc tgcccattga gattgtagtc gctcagcgca
     8281 aagcgggtgc cttcgtgcac ggcctgatcg tcgtcgatca ccgcaacctt ccatctgcgc
     8341 gtcgtggatg cctccggagc taccccggaa tcgtcgatca ggtggaggac atcgtcctgt
     8401 tcggccattg cggggtcccg tcgcttgctt ctgtggagcc gcccttcgcg gccctcggca
     8461 tgataatgcg aaatgtggtg ccttgtccca gtcttgaatc cagcatcatc cggccgccaa
     8521 gctgctgagt gacgagatta tagacgatgt gcaggccgag cccggtgccg ccctcgttgc
     8581 ggcgcgtggt aaagaacggg tcgaaggcct gccgctgcac gtccggcgtc attccggccc
     8641 cgttgtcggc aaaaatgatc tcgacgtcgt caacgccgcg ggcccgcgct gaaatcgaga
     8701 ttttcccgga acgcccgtcg gggaaagcat gatttgctgc gttgaggaac aaattcgtca
     8761 gaatctgccc ataggaaccc ggataaccgt cgatcaagag gccctcgggc acgtcgaccg
     8821 acagggcgat cgccgccttt ttcagaaccg gccgcaggct ggcgatgatc tggtcggtcg
     8881 cctcgcccag gctgaactgc cggcgttcgg catgcgagcg atcgaccgcg acctgcttga
     8941 aggactggat caactccccg gcgcgatgca ggttcgccac cagctgttgc gcggcgtcac
     9001 gcgagttccg gacgaattcc tccagttgag accggcgcaa cggaccgtcg gatctcaatt
     9061 cggcctcgaa catttcagcg cggcgggcga aactcgacgc caccgtcagg ctgatgccga
     9121 tcgggttgtt tacttcgtgg gcgacaccgg caaccaggcc gccgagcgcc gccagacgtt
     9181 cggcgtcgat caggttttgc tgggcggcgt tgagttcgaa cagggcgccc tcggcctttt
     9241 ccttggaggc gcgcaactcg tcttcggtct tgcgcttggc gatggcgttt tcgcggaaga
     9301 cttccaccgc gcgcgccatc gcgccgactt cgtccctggc atcggttccc tgcacgcgcc
     9361 ggtcgtaatc gcccgacgtg atcgcgtgca tcgccgtcat gatctgcccc agcggcagcc
     9421 ggatgctcag cgcgatcagg acgcccgcgg tgaggatgat gccgagaaag atcacggcga
     9481 tcgacagcac ccggcgggat atgtcggcca gcgtccgatc gaatgtctcc tgcgcctttt
     9541 gctcgcgctg ccgcatcttg accgacaatc gatcgatcgc gccgatggcg tcggcctggc
     9601 tggcgtcgat ggtgttgcgt aacagctcgg tccggctcgc gagctgctcg gataatttcg
     9661 cgaggccctc gcgcagcgcc acggtccgcg cattcagccg ctgcagggcc atgcgctgca
     9721 ggtcgttgtc ggcgaggtcg gacatcaccg ggatggtctt ctcgatcgtg tcggtgttgc
     9781 ggcgggcttc ctcggccgac gccgtcgcca gcgaaagata ataggcgttg gcggcgacca
     9841 gcatcgcggt gaacgcctcg cgcgactttc caagcgacgg ccagatcagg gcatcgcggt
     9901 gccccgtcgc gccctcgatg atcgagtaaa gcccggccat gtcgcgggcc ggggtcaaga
     9961 cctgctcgtc ataggtcttc gaaatggtgg cctggacgcc gcgcaattcg ccgaaaccat
    10021 tgaggaagcg ctcggtgacg cgttccagct cctccaccga tcccgacagc atcgggtcgg
    10081 tcgacgcgcg cgtggtgagg gtgccgagaa cggcctcgcg caacagcaag atctccgcga
    10141 acagttcggg gctcgactgg ttgatgtagc gatggatcag gttctgcagg cggccggttt
    10201 cgctctccag catcgccagg atcttgtccg actcccgcgc ttgccggacg tcgtcccagg
    10261 ccgagccgag cacctgggca ccgttccata tcatcgcgga aagcaccacg acgaccgccg
    10321 agttcagcgc ggcaatcgac aggatgcgcc agcggatcgg caccgcgcgc agcagcccga
    10381 cgatgcgaaa ccggccgcgc gcggcgaagc ccgccagccg ttccgcagtt tcgtcgcgtt
    10441 ggcgtatacc gaagaccaac tctcactcca ccttgcgaat tcgctcgatc agttccgcga
    10501 ccgcgggatc gcgttgcgct ttttgataga ttccggccgt cgcggcttcg aagggcttgc
    10561 ggtcgaaatc ggtgacgatc gtgaccccct cggcctccgc ctgccgccgc gactgctgct
    10621 cgagatcctt ccacttgtcg tgcatgaagt gactggaccg tatcgcggcg tcgcgaaaaa
    10681 tcgttcggtc ctccgccgac aggctttccc aggccttctg cgacatcacc agcacttccg
    10741 gtgacatcgc gtgctcggtc agggtgtaat agccggcata tttgtaatga cctgacgtga
    10801 caaaggaggg ccagttgttc tccgcgccgt cgatcagcgc ggtggaaagc gcggtgagaa
    10861 cttgcccata gggcagctcg atcggctccg cgccgagtgc cttgatcgtg tcggacatca
    10921 gttcggattg ttgcacgcgc agccgcaagc ccctgatgtc ggcgatcgag cgcaccggcc
    10981 tgacgctgtt gtagatcgat cgcgccccgg aatcgtaaaa cgccaggccg acaaagccat
    11041 agggttcgaa actgttcagg atttcgttgc cgatcggccc gtccagcact ttctgcaaat
    11101 gctcgatgga gcggaacaga aacggcatcg ccagcacgtt catcgccggc acgaaattcc
    11161 cgatcagtgc gccgttggtg cgattgagat cgatggcgcc ggcgcgggtc tgctcaatgg
    11221 tttccttttc ctcgccgagc tggcgcgaat ggaacacccg gatctggtgg cggccgccgc
    11281 tgcgttccgt gaccatgcgg cccatatagc gcagtgcctg gacggtcgga taatcctcgt
    11341 tctggctgtc ggcggcgcga aattcccgtc cgacagcgct cgtccagacc gccgccaaaa
    11401 gcagcgcgac gacaggtgcg atggtccgcg aaaggtcggc gcggctgtac acgggcaact
    11461 gcatcccctt atgatggtgt cggctaaggc aagaaaaggt tcaacgcgat tttatagaag
    11521 ccgccgaggt atagccatac tgcctatgcg gctatcgtta atggtgcacc gcgaccgccc
    11581 ttacggattg aaccgccggt gctaggcttg acgagggcgc gtgacgcatg aaaaccgttt
    11641 cgaccgcggc agatcggaag gtcctaatcg tgaaatcgac cttgaggttc ctgcaactcg
    11701 cggctgtcgt cgcggcggtc actttggcct ctccggctct cgccgtcacc gatatcatgt
    11761 ggtggcatgc catgtcgggc gagctcggga ggcagctgga caagctcgcc gccaatttca
    11821 acgcatcgca atccgaatac cggatcgtgc cgagttacaa aggcaattac accgaaaccg
    11881 taacggcggc gatcttcgcg tttcgttcgc ggagccagcc cgccatcgtg caggtcagcg
    11941 aaatcgcgac cgcgaccatg atggctgcgc gaggcgcgat ctatcccgtg ttcgaactga
    12001 tgcgggacga acacgaggca ttttccacgg cggcgtacct tccggccatt accggctatt
    12061 acgccgacgt cgcgggcaac atgctgtcgt tcccgttcaa ctcctcgacg ccgatcctgt
    12121 attataacaa gaacctgttt cgcgccgccg gcctcgaccc cgaagtggcg ccgaagacct
    12181 ggagcgaagt cggcgcggcg gcgaagcggc tgcgtgccta tggtgccgtt tgcggcttca
    12241 cgacgtcctg gccctcatgg atcaacgtcg agaatttttc ggcgttccac aatctgccga
    12301 tgtcgaccaa ggccaacggc tttgacggtc tcgatgcggt gctgactttc aacaatccgc
    12361 tgatggagcg ccacatcgcc caactcgcgg agtggcagac cagcaagatg ttcgactaca
    12421 gcggccgcgc cacctcggcg gagccgcgat ttcaaaatgg cgaatgcggg atcttcatcg
    12481 gctcatcggc gacacgcgcg gatatcaggg ccaattcgaa attcgaggtg ggatacggca
    12541 tgttgccgta ttggcccgat gttgaaggcg cgccgcaaaa caccacgatc ggcggcgcga
    12601 cattgtgggt gctgcgggac cggccgcgcg aagaatacaa gggcgtcgca aaattcttcg
    12661 gatacctgtc gaagccggag attcaggcgg cgtggcacca gaataccggc tatctgccga
    12721 ttacccgcgc ggctttcgaa ttgacccgcg cccagggctt ctacgatcgc aatcccggca
    12781 cggcgatctc gatcgagcag attaccctga aaacaccgac cgaaaattcg aggggagttc
    12841 ggcttggatc cttcgttctg att
//
LOCUS       DATNOI010000009         3278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1781863,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000009 DATNOI010000000
VERSION     DATNOI010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781863"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1365
                     /locus_tag="VN968_00305"
     CDS             151..1365
                     /locus_tag="VN968_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL27725.1"
                     /translation="MKSIAAAVASSLVLAASGTAANAQISDDVVKIGVLTDMSSLYAD
                     ATGKGSLAAVQMAVADYGAKVKGKPVEVVSADHQNKPDIGLGIARNWYDNEKVDAIFD
                     VPTSSVALPVSALTREKNKININSGGGSSDITGVACSPNTVHWTYDTYALSNVAGRAM
                     VKRGEDSWFFITADYAFGMALERDAANVVKESGGQVVGNVRHPLNSSDFSSFLLQAQA
                     SKAKVVALANAGGDTTNALKQASEFGITKGGQKMIALLQEITDTHALGAKETQGLIVT
                     DAFYWDMNDETRAFSKRFNEKIGHMPSMIQAGLYSATMHYLKAIEAIGTDEAPKVMEQ
                     MRATPINDFFAHGGKIRIDGRMVHDMYLFEVKKPEESKGEWDLYKLLATVPGDEAFRP
                     LYKGGCPLVKMN"
     gene            complement(1504..2040)
                     /locus_tag="VN968_00310"
     CDS             complement(1504..2040)
                     /locus_tag="VN968_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HXL27726.1"
                     /translation="MLAKSMTAGLIGSALLASVAFAQTPTATTDRANTPPAAASESAF
                     QDNWRASKVVGVSVYNDNNESLGSISDLLMDKSGNIKAVVIGVGGFLGVGEHLVAIPF
                     DKIKFVNEPVAYTGVAGAPNAPGSRPASSTTTGAAPTNTPPAAATTPKSNPWYPDHAV
                     FNATKDELKAMPEFKYST"
     gene            complement(2226..2600)
                     /locus_tag="VN968_00315"
     CDS             complement(2226..2600)
                     /locus_tag="VN968_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HXL27727.1"
                     /translation="MTDVMTKPHQLIASDRVEGTAVRRPNGDKLGHIERLMIDKVSGK
                     VSYAVLSFGGFLGMGANLLPLPWARLAYNTRFEAYELDIDDEELKRAPSFRSDLDFDW
                     GDRSQEAELHRYYGTPPYWGGF"
     gene            complement(2839..>3278)
                     /locus_tag="VN968_00320"
     CDS             complement(2839..>3278)
                     /locus_tag="VN968_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27728.1"
                     /translation="QGGQSSGQSGGTQSSPGDLKSKIDDLIAGEVSSGKLTSDQATEL
                     QGVFKAAFANGPDGAGGPGGAGGAHHGHHGGPPPTDSSSSSDNTSSSSGTSSASDILQ
                     QFLQSLQDSLSASSSAPYSATGTSGTGSNSSTSFSALLIDYQT"
BASE COUNT          643 a          994 c         1043 g          598 t
ORIGIN      
        1 taacatcggg accacggctt cgaaaggcgc aatccagaga cggtggcggg ctgagcctgc
       61 ccaaaaaccg gctttgcggg tttgggcgaa ggcggctaaa agacagcctc aaaccaagcc
      121 ctaaacaaga acgatcaggc aggaagaaac atgaagtcaa ttgcagccgc ggttgcatcc
      181 agtctcgttt tggcggcgtc cggtaccgcg gcgaacgcgc agatctctga tgacgtcgtc
      241 aagatcggcg tcctcaccga tatgtccagc ctctatgccg acgcgaccgg caagggctcg
      301 ctggccgccg tccagatggc ggttgccgac tacggcgcca aggtgaaggg caaaccggtc
      361 gaggtggttt ccgccgacca tcagaacaag cccgacatcg gccttggcat cgcgcgcaac
      421 tggtatgaca acgagaaggt cgacgcgatc ttcgacgtgc cgacctcgtc ggtcgcgctg
      481 ccggtttccg cactgacccg cgagaagaac aagatcaaca tcaattccgg cggcggcagc
      541 tccgacatta ccggcgttgc gtgttcgccg aacaccgtgc actggactta cgatacctac
      601 gcgctctcca acgtcgccgg ccgggcgatg gtcaagcgcg gcgaggacag ctggttcttc
      661 atcaccgcgg actatgcgtt cgggatggcg ctggagcgcg atgcggcaaa tgtcgtcaag
      721 gagagcggcg gccaggtggt cggaaacgtc cgccatccgc tcaattcgtc ggacttttcg
      781 tccttcctgc tgcaggcgca ggcctccaag gccaaagtgg tggcgctcgc caacgccggc
      841 ggcgacacca ccaatgcgct caagcaggct tccgaattcg gcatcaccaa gggcggccag
      901 aagatgatcg cgctgctgca ggaaatcacc gacacccacg cgctcggcgc caaggaaacc
      961 cagggcctga tcgtcaccga cgccttctac tgggacatga acgacgagac ccgcgccttc
     1021 tcgaaacgct tcaatgagaa gatcggccac atgcccagca tgattcaggc cgggctgtat
     1081 tcggcaacca tgcattacct gaaggcgatc gaggcgatcg gtaccgacga agcgccgaaa
     1141 gtgatggagc agatgcgggc gacgccgatc aacgactttt tcgcacatgg cggcaagatc
     1201 cgcatcgacg gccgcatggt ccacgacatg tatctgttcg aggtgaagaa gccggaagaa
     1261 tcgaagggtg aatgggatct ctacaagctg ctcgccaccg tgcccggcga cgaggcgttc
     1321 cgtccgctct acaagggcgg ctgcccgctg gtgaagatga attgaggtgc aggggtcttt
     1381 ctctcgtcat ggccgggctt gtcccgggca tgacgagtag cagaaccgtg cgacataagc
     1441 ccaaaaacgc gcccggcaaa accgggcgcg cttcctattt gacgggtctg ttcaggcgtt
     1501 gccttaagtc gaatacttga attccggcat cgccttcagc tcatccttgg tcgcgttgaa
     1561 cacggcgtgg tccggatacc acgggttgga cttcggcgtt gtcgccgcgg ccggcggagt
     1621 gttcgtgggt gcagcacccg tggtcgtcga tgacgccggc cggctgccgg gcgcgttcgg
     1681 cgcgcccgca acgccggtgt aggcgaccgg ctcgttgacg aacttgatct tgtcgaaggg
     1741 aatggcgacc agatgctcgc cgaccccgag gaagccgccg acgccaatca ccaccgcctt
     1801 gatattcccg ctcttgtcca tcaacagatc gctgatcgaa ccgaggcttt cgttgttgtc
     1861 attgtagacg ctgacgccga cgaccttgga cgcgcgccag ttatcctgga acgcggactc
     1921 cgacgccgcg gccggcggcg tgttggcgcg gtcggtggtt gcggtcgggg tttgcgcgaa
     1981 cgccacggac gcgagcaacg ccgacccgat cagaccggcc gtcattgatt ttgctaacat
     2041 gggctgtctc cctgagatga attggtgggg agaaaacacg ccgatccgtc ggtcgttcca
     2101 atcgccgaac acgatttaag cgttagcggt gctgacgatg ggagagttcc gtgcaacaac
     2161 gcagtgcaca cgcgcgcacg gcgacggcgt gcaatattgc ggctatgccc acccttcaat
     2221 ggcgatcaga aaccgcccca ataaggcggc gttccgtaat agcggtgcaa ttccgcttct
     2281 tgcgaacgat cgccccagtc gaaatccagg tcggagcgga acgacggcgc gcgcttaagt
     2341 tcctcatcgt cgatgtcgag ctcgtaggcc tcgaacctgg tgttgtaggc gagacgcgcc
     2401 catggcagcg gcagcaggtt ggctcccatg ccgaggaacc cgccgaaact gagcaccgcg
     2461 taggacacct tgccgctgac cttgtcgatc atcagccgct cgatgtggcc gagcttgtca
     2521 ccgttcggcc gacgcaccgc agtaccctcg acgcgatcgc tcgcgatcaa ttggtgtggt
     2581 ttggtcatga cgtcggtcat cgcaccctcc ttttgggttg atgaaacaaa tcaacgccat
     2641 aacggcagat cggttccgtc ggcaccgggc caaatcgcct gaaggcaatc gcttgccgga
     2701 gggcgaagga ggaagcaaat gccaccttcc ggttctcccc ggccaggtcc ggcgggccag
     2761 ggggctgcac atgcgatcat cctttcccgt tctgggaagg atgacgaggt gcgtcggggt
     2821 gtgcgtcgcg gcctgaggtc aggtttggta atcgatcaaa agcgccgaga acgacgtgga
     2881 gctgttgctg ccggtcccgg aagtgccggt cgcgctatat ggcgcagatg acgaggccga
     2941 cagcgaatcc tgcagcgact gcaaaaactg ctgcagaatg tcgctggcgg agctggtgcc
     3001 cgacgagctg ctggtgttgt cggatgagga cgaactgtcg gtcggcggtg gtccgccgtg
     3061 atggccgtgg tgcgcgccgc cggcgccacc tggaccgccc gcgccgtcgg ggccgttggc
     3121 gaaggctgcc ttgaaaacgc cctgcagttc ggtggcctga tcgctggtca atttgccgct
     3181 ggacacttcg ccggcaatca ggtcgtcgat cttcgacttc agatcgccgg gagaggattg
     3241 tgtgccgccg gactgaccgc tcgactggcc gccttgca
//
LOCUS       DATNOI010000010         2614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1781432,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000010 DATNOI010000000
VERSION     DATNOI010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1781432"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VN968_00325"
     CDS             complement(<1..1239)
                     /locus_tag="VN968_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXL27729.1"
                     /translation="MDHEQLVRKAGSGDVKAFVELTRRFQQFAFGSALALVRDFQHAE
                     DVVQESFIAAWSALPSLADPAAFPGWLRGIVRHHAFRVLRRKRLASVPLAEAEGVPDE
                     APLPDHILERRRQAAAALAAISELPDKLREPAALFFVHECSHQDVATFLGLSVATVNN
                     RLHAARSRLKQRMLTMVSDTLHSHALPDDFANRIGRLIETRGDVVDALFDPAALPDIL
                     TELTVSDEARKKSLKVHVMQRPGGGVVRGLTTSVAPPLPKGATVLSSRRQSPVPIDQV
                     QLDRLVPLLAATATDSRPSDEVVETGIKVIDVICPIRAGGSVAIAGEYGTGITVVMEE
                     IVRRISKSAHPVTMFVLMPPPSEIWPPSLDESRSFAEELKQEGYSEGTVGSVQTFFVR
                     GIEGPWTAQTLAALAPIDTVV"
     gene            complement(1351..>2614)
                     /locus_tag="VN968_00330"
     CDS             complement(1351..>2614)
                     /locus_tag="VN968_00330"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27730.1"
                     /translation="AALRMGASHVDAIEIDPAIAFLGKASHPEHPYDDPRVTLNINDA
                     RNFFRTAHQQYDLIIYGVLDSHTALSHASNLRVDSYVYTREGITEAFRLLKPDGVMSI
                     AFALPNESLGFKLSHILQDIPGAGKPLAVRVGYDSKTTTAFITQRGHDVAMPDAKAFD
                     VFGFTDVSGHFAQAYPAESIPTDDWPFFYMLNRTYPVSYMIALGMVLLLSYMFVRKTI
                     GWSEPVERGYLPFFFLGSGFMLVETKAITELGLHLGGTWFVIAATILMVLLMAFLANL
                     MVQRKLVPRTSLAYAGLLASLVIGYFSARNHELISFASPLLTLAVASVLLTIPLFFSG
                     MVFSTLIGKPNINISTALAYNLMGALFGGLMEYNSMYFGFAFLYLLAIGFYGLAWLFS
                     LEPTAAARRLTMLSQDPAPQEAPPKARA"
BASE COUNT          541 a          784 c          841 g          448 t
ORIGIN      
        1 aacgacagta tcgatcggcg cgagcgccgc cagcgtttga gcggtccatg gcccctcgat
       61 gccgcgcacg aaaaaggtct ggacgctgcc gaccgtgccc tcactatatc cttcctgttt
      121 cagttcttcc gcaaacgaac ggctttcgtc cagcgaaggc ggccagattt cggaaggcgg
      181 cggcatcagc acgaacatcg tcacgggatg agcgctcttg ctgatgcgcc gcacgatctc
      241 ttccatgaca accgtgatcc cggtgccata ttcaccggcg atcgcgacgc tgccgccggc
      301 gcgaatcggg cagatcacat cgatcacctt gatgccggtc tcgaccacct catcggatgg
      361 ccggctgtcc gtggcggtgg ccgcgagcag cggcaccagg cggtcgagct gcacctgatc
      421 gatcggcacc ggagactggc ggcgcgaact cagcacggtc gcgcccttcg gcagcggcgg
      481 tgcaacggac gtcgtaaggc cgcgcaccac gccgccgccg ggacgttgca tcacatgaac
      541 cttgaggctt ttcttgcgcg cttcgtcgct gaccgtcagt tcggtcagga tatccggcag
      601 cgcagccgga tcgaacaacg cgtccacgac atcgccgcgc gtctcaatca agcggccaat
      661 ccgattggcg aaatcgtcag gcagcgcatg ggaatggagc gtatcgctga ccatggtgag
      721 catcctctgt ttgaggcggg aacgggccgc atgcaggcga ttgttgacgg tcgcaactga
      781 cagcccgagg aaggtggcga catcctgatg cgagcattca tggacgaaga atagcgccgc
      841 aggttcgcgc agcttgtcgg gcagttccga aatcgctgca agcgcggccg cggcctgccg
      901 gcgccgctcg aggatgtgat ccggcagcgg cgcttcatcc ggcacccctt cggcttccgc
      961 gagcgggacg gacgcgagcc gctttcgccg cagcacgcga aaggcatgat gccggacaat
     1021 gccccgcagc cagcccggaa acgccgcggg atcggcgaga ctcggcaagg cagaccatgc
     1081 tgcgatgaag gattcctgaa ccacgtcctc ggcgtgctga aagtcacgca ccagggccag
     1141 cgccgagccg aatgcaaatt gctggaaacg ccgggtcagt tcgacaaacg ccttgacgtc
     1201 gccgctgcct gcctttcgca ccagttgctc atgatccatg gccatgccct cttcacctca
     1261 aggtgccagg atcgacggtt gacctttagg ggcaaagcaa tttttttgac gccgcatgaa
     1321 aaaagccccg acctttcggc cggggctttt tcacgctctt gcttttgggg gcgcttcttg
     1381 aggcgccgga tcctggctca gcatagtcag ccggcgcgcc gcagcagtgg gctcgagcga
     1441 aaacagccag gcgagcccat agaagccgat cgcgagcaaa tagaggaacg cgaagccgaa
     1501 atacatcgag ttatattcca tcaacccgcc gaacagcgcg cccatcagat tataggcgag
     1561 cgcggtggag atgttgatat tgggcttgcc gatcaaggtg gagaacacca tgcctgagaa
     1621 gaacagcggg atcgtcaaga gcacgctggc gaccgcgagc gtcaggagcg gcgaggcgaa
     1681 cgagatcagt tcgtggttgc gggctgaaaa atatccgatg acgaggctcg ccagcagccc
     1741 ggcataggca aggctcgtgc gcggcaccag cttgcgctgg accatcaggt tggcgaggaa
     1801 cgccatcagc agaaccatca ggatggtcgc ggcgatgacg aaccaggtgc cgccgagatg
     1861 caggccgagt tcggtgatcg ccttagtctc caccagcatg aagcccgagc cgaggaagaa
     1921 gaacggcagg tagccgcgct cgaccggctc gctccagccg atggtcttgc gcacgaacat
     1981 gtagctgagc agcagcacca tgccgagcgc gatcatgtag ctcaccggat aggtgcggtt
     2041 cagcatgtag aagaacggcc agtcgtcggt cgggatgctt tcggcgggat aggcctgcgc
     2101 gaagtggccg gacacatccg taaagccgaa gacgtcaaaa gccttcgcgt ccggcatggc
     2161 aacatcatgc ccccgttgcg tgatgaaggc cgtcgtggtc ttcgaatcgt aaccgacgcg
     2221 gacggccagc ggctttccgg cgcctggaat gtcctgcaag atgtgcgaca gcttgaagcc
     2281 cagcgattca ttcggcagcg cgaacgcgat cgacatcacg ccgtcgggct tgagcagcct
     2341 gaaggcctcg gtgataccct cgcgcgtgta gacgtaggaa tcgacgcgca gattggaggc
     2401 atggctcagc gcggtgtggg aatccaggac accgtagatg atcaggtcat attgctgatg
     2461 cgcggtgcgg aagaaattac gcgcatcgtt gatattcagc gtgacgcgcg gatcgtcgta
     2521 gggatgttca ggatgcgagg ccttgccgag gaaggcgatg gccggatcga tttcgatggc
     2581 gtcgacatgc gaggctccca tgcgcagggc cgca
//
LOCUS       DATNOI010000011         2016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1235901,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000011 DATNOI010000000
VERSION     DATNOI010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235901"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VN968_00335"
     CDS             <1..1194
                     /locus_tag="VN968_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HXL27731.1"
                     /translation="APGSGIAATSSTDRLSPNGNNYGSSSNGFGSRSQNGAAASNTPG
                     FGATAQPAAANTSGVGALDGGTRTAGAGGQPVLQNVRITADTVNNTLLIYADQESYRI
                     LESTLQQVDQPQLQVAIDATIAEVTLTDELSYGVQAFLTSRNLGLKPDTGSILNTQSG
                     AAPAAAAAGTVANAFINRAFPGFNFLIGSEAQPSAILDALHTVTSVKVLSNPSLVVIN
                     NQAATLQVGDVVPVSTGSATVLTTNNTVVNTIDYRNTGIILRVSPRINVNGNVRLDVE
                     QEISNVSPQTAASLTPTVSERKVRSSISVATGQTVLLAGLISEQQNNTRNGIPLLDRI
                     PGLGDGFSHQDKKNTRTELIIFIRPQIIRDGTDAHFVAEELRSKLRGNLGASLVNEPR
                     VQGVR"
     gene            1243..1710
                     /locus_tag="VN968_00340"
     CDS             1243..1710
                     /locus_tag="VN968_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL27732.1"
                     /translation="MIWLSGAGTLHTAHADGLTRATGAYARGDYVRAVHALSPLARRG
                     NAHAQALLGFLYENGFGVPQAYDAAADLYQRAAGQGDVFAQARLGLIYDKGHGVPQNA
                     ILSYKWLDLAAAHASKRERDYYIRLRNAVASKMSSEQIVEGQRLALIWVPGRW"
BASE COUNT          422 a          639 c          594 g          361 t
ORIGIN      
        1 gcgccgggat ccggaattgc cgcgacctcc agcaccgatc gcctgtcgcc gaacggcaac
       61 aattatggtt cgtcatcgaa cggctttgga tcgcgctcac agaacggagc cgccgcaagc
      121 aacacgccgg gctttggagc cacggcgcaa ccggcggccg cgaacacctc cggcgtcgga
      181 gcgttggatg gcggcactcg aacggcaggc gccggcggcc aaccggtttt gcaaaatgtt
      241 cggatcacgg ccgataccgt taacaacacc ctattgatct atgccgacca ggaaagctac
      301 cgaatcctcg aatcgacatt gcagcaggtc gatcaaccgc agctgcaagt ggcgatcgat
      361 gcgaccatcg ccgaagttac cctcaccgac gagttgagct acggcgttca ggccttcctg
      421 acgagtcgca atctcggttt gaaaccggac accggctcca tcctcaacac gcaatcggga
      481 gcggcgcccg cggcagccgc ggccggaacg gtcgccaacg cgttcatcaa ccgcgcattc
      541 ccgggattca atttcttgat cggatcggaa gcccaaccga gcgccattct ggacgccctt
      601 cacaccgtca ccagcgtcaa ggtcctatcc aatccgtctc tggtcgtgat caacaaccag
      661 gccgccacgc tccaggtcgg cgacgtcgtg ccggtttcca ccggcagcgc cacggtgctg
      721 acgaccaaca acacggtcgt caacacgatc gactatcgca acaccggcat cattctgcgc
      781 gtgtcgcccc gcatcaacgt caatggaaac gtcaggctcg acgtcgagca ggagatcagc
      841 aacgtttcgc cccaaaccgc cgccagtttg acgccgaccg tttcggaacg aaaggtcaga
      901 agttcgattt cggtggcgac cggtcagacg gtcctgctcg ccggattgat cagcgaacag
      961 cagaacaata cgaggaacgg gattcccttg ctcgaccgga ttccgggcct gggcgacggc
     1021 ttttcgcatc aggacaagaa gaacactcgt accgagttga tcatcttcat ccgtccccag
     1081 atcatccgcg acggcaccga tgcgcatttc gtggccgaag aacttcgctc gaaattgcgg
     1141 ggaaatctcg gggcgagcct ggtaaatgaa ccacgggtcc agggcgtgcg ctgaggtgag
     1201 gagctgagtt cagtgcgcta cactttatcg accattttat ccgtgatttg gttgtctggc
     1261 gccggtacgc tgcacacggc gcatgccgat ggattgacac gtgcgacagg cgcctatgca
     1321 agaggcgact acgtccgagc cgtccacgcg ctgtcgccgc tggcgcggcg cggaaacgcc
     1381 catgcgcagg cgctcctggg ctttttatat gaaaacggct tcggtgtgcc gcaggcctat
     1441 gacgccgccg ccgatcttta ccaaagagcg gccgggcaag gcgacgtctt tgcacaagcg
     1501 cgactcggct tgatctacga caagggccac ggcgtgccgc aaaacgcgat tctgtcctac
     1561 aagtggctcg atttggccgc cgcccatgcc tccaagcgcg agcgcgacta ctacatcagg
     1621 cttcgcaatg cggtggcttc gaaaatgtcc tccgaacaga tcgtcgaagg ccaacggctt
     1681 gccttgatct gggtgcccgg acggtggtga tagtgccacg tcctttcctg aagcgaagtt
     1741 agaaccgaga gctgcggact ctgctcaaac gacagggggc ccaccgggcc ccctgaagga
     1801 aggttgagtg acggcggtcg gctggctttg gtttacccgt cagccgaccg cgcgtaccga
     1861 gacgtcacgg cgaaacaacc accaccggca ccgaagattc gcgacgctcg cggcgggcga
     1921 aggagaactc taaggtgttg ttgtcgccat gctgctgcgt gaagaagcca cggacagcac
     1981 cagcgaggct ttccttggtt ccttgcagtc cggccg
//
LOCUS       DATNOI010000012        27489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1070194,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000012 DATNOI010000000
VERSION     DATNOI010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 27489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27489
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070194"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VN968_00345"
     CDS             <1..349
                     /locus_tag="VN968_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27733.1"
                     /translation="EDFGMLDGLRFDAELGTTGERAGGNGADGKYVEPKAHIIITTQT
                     ILARWLRAHKDWWDKGLKNVPQQIAAALKDESFYTQAMSTDAAVVNFDPLPIAKPASA
                     TFAYGMLAAAMGR"
     gene            complement(467..718)
                     /locus_tag="VN968_00350"
                     /pseudo
     CDS             complement(467..718)
                     /locus_tag="VN968_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
     gene            complement(754..1464)
                     /locus_tag="VN968_00355"
     CDS             complement(754..1464)
                     /locus_tag="VN968_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL27734.1"
                     /translation="MVRRVQGIVSHTLVDGIDLSVAKGEFLAITGPSGSGKSSLLYLL
                     GLLDAPTEGEVIICGQATSKLSETERADVRLTKCGFVFQFHFLLPEFTALENVLLPMR
                     AYGRMSTKDMNERAMSLLTSLGLGEQTGKRPNQLSGGQRQRVALARALANRPEIIVAD
                     EPTGNLDTASTEQVFGILRDIANGGQTVVVVTHDAALAARADRRIHIVDGKIHETTFG
                     GDRKPEIAAVTPTAECPS"
     gene            complement(1491..2735)
                     /locus_tag="VN968_00360"
     CDS             complement(1491..2735)
                     /locus_tag="VN968_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL27735.1"
                     /translation="MATNLLLSIAWTHVTTRIRQTLVGMCGVAMGVGFTIMMAGLMQG
                     SQIDFLRQLVDTMPHVMVGDERRSVPSQPADQEYAAVGMPNVANVNRRPGIKYPDTVM
                     ASLKSWLPGDVAPSVKTTAILNHGDARVGVSLIGIDPRLEVRVSKLGSQMLEGQLTDL
                     SKAANGIIIGAALAEKLAVKYGNTVLLGTGGGAPVSATVVGIFKSGIKSVDEGQIYSL
                     MNVAQTLIGQNGLVNQLRLRLSDPLIAQQVATRVEAQIGYKSVSWQEANSDLLGAFAT
                     RDFIVLTVMGAMLLSSSFATYNIISTITHEKRHDIAIMKSLGMREYSVRRIFIIESTM
                     MGSVGILFGWLLGYLLCYGWSQIKIFNVLTGATVPLEIYYSPTHYLATGAISIFCCAV
                     AAFFPARKATRVHPVEIIRGAS"
     gene            complement(2741..3952)
                     /locus_tag="VN968_00365"
     CDS             complement(2741..3952)
                     /locus_tag="VN968_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXL27736.1"
                     /translation="MSSRGLVWSGSVLAVLAVAAVGGAYWKYPDFFQPPTVTTGVAVL
                     GPVSEAVYGTGTVEPERWAKVVPLQRRRLVELCRCEGQAVKAGQVLGRQDDAEEVSAL
                     HELEINNDQLARDLDRASRDRDKSEAQKTEYEQRSTQFEQSKSRMSAQKARIDSLVLR
                     APLDGMVLRRDGEVGEIVGPTEVLFWVGPPTPMQVVAEINEEEITRIAAGQKAFLRSE
                     AFSAQALRATVSQITPKGDPTRKTFRVYLRLPPDTPLRIGMTVEVNIIYREKAAAVVV
                     PAEAVAGNAVQVVSGGQIQRVPVTIGVRGSRNVEIIGHVSKDTTVLSPARADLADGTR
                     VRVENVAAKPAPAAPADIAPVPSPALDRAPAAAVVASAADADDAVISAAISAHVDSVV
                     NDARRNVGKYR"
     gene            complement(3976..4668)
                     /locus_tag="VN968_00370"
     CDS             complement(3976..4668)
                     /locus_tag="VN968_00370"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL27737.1"
                     /translation="MKRVFLAMLCGAALIASSVAHAGDAVSDKEMADRYSQAALSGDS
                     DAQFYLAALYSSGVGRPRSDEEAFRWFSRAADQGHSHAMLVLSGMYAVGRGISKDNFN
                     AYKWAYIVAVGSRVDEFRNGSRQLMGVLETRMTRDDVERAKAEAGRWHATSSQPKPAV
                     APAPADSLTRDYQPSPQLSPAVSKQPPATTSNNSSVTGSLPGPRNTSERKNDMDALLD
                     RVPTGLRKRFGF"
     gene            4825..5181
                     /locus_tag="VN968_00375"
     CDS             4825..5181
                     /locus_tag="VN968_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27738.1"
                     /translation="MGYFCKTFVATRILILLGTVVATPVAASTFDGAWNVQIASANAA
                     CGGATISIGIANGQVESSNALMKATGRVADAGGISVTLTSGIKRAVGSGHLSATSGSG
                     TWRGALCSGTWTAQRI"
     gene            complement(5255..5659)
                     /locus_tag="VN968_00380"
     CDS             complement(5255..5659)
                     /locus_tag="VN968_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL27739.1"
                     /translation="MNIHLAGSRPTRLAPKEYFTGTVWQDPIIAAEVPARIVSNRVSF
                     EPGARTNWHSHPLGQTLYVISGVGRVQSKGGPIREIRAGDVVWIPPGEKHWHGGSPTN
                     GMIHIAMQESLDGNHATWMEPVTDAEYGAPVG"
     gene            complement(5702..6400)
                     /locus_tag="VN968_00385"
     CDS             complement(5702..6400)
                     /locus_tag="VN968_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HXL27740.1"
                     /translation="MSLTPEAIKTLSHVSTATITTVLLKKGLRNVWLRGSRPLRPGQK
                     RLVGPAFTLRFVPAREDLATPESWSSPISTRTAIEAMPEGCIAVVDAMGITDAGIFGD
                     ILCARMVKRGVAALVTDGVVRDLEGVLGTGLPVWCDGFAAPPSVAGLTFVGWGEPIGC
                     GGVAIFPDDIIVADQDGAVVIPKAFLDLILAEGAEQERMEAWIVEQVNGGAVLPGLYP
                     MNAETKARYAASKK"
     gene            6642..7373
                     /gene="pxpB"
                     /locus_tag="VN968_00390"
     CDS             6642..7373
                     /gene="pxpB"
                     /locus_tag="VN968_00390"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HXL27741.1"
                     /translation="MAATRSPPRLLPSGDSAITVEFSRSIDEMANQRVLALDRTLARE
                     TITGVTETVPTYRSLLVHYDPVQIDFDTLSGKLVTLALLPVPPVTKMRRWRIPVAYGG
                     EHGIDLEDVARTLKSTPENIVARHVAGDYRVAMIGFTPGWSYLSGLDASLQMPRLQNP
                     RLVTPAGTVSIGGVQTGVQCLAGPSGWHLLGRTAVRTYQLHRDPIFLLEPGDAVTFSA
                     VDAKTFAEQDRAAAAGEIVAELMTS"
     gene            7370..8482
                     /locus_tag="VN968_00395"
     CDS             7370..8482
                     /locus_tag="VN968_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HXL27742.1"
                     /translation="MSKLAISAIGPASSVQDGGRYGAQRYGLTPSGAMDRLALAAANS
                     LVGNATFAAAIEIGPFGAKFTAREGAVRIALAGASRNADISGRAVVSNTSTTMADGET
                     LTLGFARGGSFSYLAIEGGIVGEPMFGSLAVNARAGLGSPYPRPLQAGDELQTAAASG
                     AAENRIDLPPPADGPIRVVMGPQDDEFGDAKALFLDREWKISSASDRMGYRLEGPVIK
                     HLHGHNIVSDGTVNGSVQVPGNGQPIVLMPDRGTSGGYPKIATLITADLGRFAQIPAG
                     TGFRFRAVSMAEAQAEARKSSEMLRTLPDRLRPIDNFALNIEALRDANVAGAAVSAVD
                     AGTWQIPSSADAPAQDWLTQTQDQPKLHSRQADTTS"
     gene            8476..9246
                     /locus_tag="VN968_00400"
     CDS             8476..9246
                     /locus_tag="VN968_00400"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HXL27743.1"
                     /translation="MMTIDLNCDLGEGFGAWEMGNDAAMIDLATSVNIACGFHAGDSD
                     TMRKTVELAKARGVSIGAHPGYRDLHGFGRRPFPGMKASEIENLVAYQIGALQAIATM
                     AGHKVTHVKAHGALSNVACEDEATARAIASAIRAVDPDLIFVVLANSKLVQAGEAANL
                     PMVHEVFADRAYEDDGSLVSRRKPGAVLHDAREIAQRVVRMVQDSAVVSVTGKVIKMR
                     TDTVCIHGDTPGAVDIARGVRQALKDGGIEVAPFKKAS"
     gene            complement(9256..9408)
                     /locus_tag="VN968_00405"
     CDS             complement(9256..9408)
                     /locus_tag="VN968_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27744.1"
                     /translation="MRELSAEARLHLYARSLSKRTGTGLHTAALYGAFAVIAAVVFGT
                     LSVHPF"
     gene            complement(9585..11189)
                     /locus_tag="VN968_00410"
     CDS             complement(9585..11189)
                     /locus_tag="VN968_00410"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HXL27745.1"
                     /translation="MNQMTPPPRLEIIPPTAPFSEGQRSWLNGFLAGLLQFDGSTPLA
                     PAQNGVLLAPSGDGDDGEAPWHDQTMPIADRMKLAEGRPLRRRMMAAMAQQDCGQCGY
                     NCNDYSDAIVSKAEPRLNLCVPGGKETARMLKALNEELEKAPPALSGVAAVPAAAAPA
                     IAVDPGRSRDNPVPATFVSRRLLNESGSEKETWHIDFDLSGCGLDYVVGDSFGIFARN
                     DLGHVDQIIALLGASHTTEVRGKTLREVLIDDVSLAPAPDSLFELISFITGGAQREKA
                     RALAQGEDPDGDAATLDVMAALQKFSGVRPHPEAFVEALEPLQPRLYSISSSHNATPG
                     KLSLTVDCVRYVVGKRKRLGLASTFLAERIKPGDQLKVYVQKAHGFALPQDPKTPIIM
                     IGPGTGVAPFRAFLLDRRATGAPGKNWLFFGHQRSSCDFFYADELNAMKTSGLLTRMS
                     LAWSRDGDKKFYVQDRMREVGRELWTWLAEGANIYICGDAKRMAKDVERALVDIVAQF
                     GARSTDEAVSFVAELKKKGRFQQDVY"
     gene            complement(11186..12976)
                     /locus_tag="VN968_00415"
     CDS             complement(11186..12976)
                     /locus_tag="VN968_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirA family protein"
                     /protein_id="HXL27746.1"
                     /translation="MKLETTTADFSDEQKRYLEGFMSGLQVGRVGRLAGGAVPAGKAA
                     PEPIGPDAAGFKAQDKVIASGKKLADQEKFKREEHPFDAYSRLKQQAVTNAPPTPADN
                     FRWRYYGLFYVAPAQDSYMCRLRIPNGILKHWQFAGMADLAERLCGPFCHVTTRANLQ
                     VREIPPKHTVALIEGIQDLGLCSRGSGADNIRNVTGTPTAGIDPQELIDTREYARQWH
                     YHILNDRSLTGLPRKFNVAFDGAGRIAVLEDTNDIAFAAVEVKDGFGVDPGVWFRLGI
                     GGITGHKDFAKETGIIVKPADATKVADAIVRVFIDLGDRTNRLKARLKYVIDGMGMEK
                     FLVLVEEKLGHALTRVPPEALALRPAFDRMAHIGIHAQKQPGLNWIGVVLPVGKLTCD
                     QMRGLAKIARDLGDGDIRLTVWQNLLISGVRDENVALATAAIETLGLAVKASQMRAGL
                     IACTGNAGCKFAASDTKRHAAEIGDWCETRVSIETPLNIHLTGCHHSCAQHYISDIGL
                     LAAKVPAGSDDDTVEGYHLYAGGGFGPDADIGQEVYRNVKAEDAPKTVEKLLKAYLAN
                     RVSAEETFLTFSRRHDGEALRKLAEAEAMT"
     gene            complement(12989..13825)
                     /locus_tag="VN968_00420"
     CDS             complement(12989..13825)
                     /locus_tag="VN968_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HXL27747.1"
                     /translation="MDYAKPADVVASMIDASLRKLALSPRDLLIRGALSGALLGAATA
                     LAFSGAITTGQPLVGALIFPVSLVMIVLLGLELVTGSFGLLPLARLEGKATWNAIIAN
                     WSWVFLGNLLGSIAFGALLAISLTNMGKIEPAGVAARVISIAEAKTVANEALGAAGMV
                     SVFVKAILCNWLVCLGVVMAMTSTSTVGKIAATWLPIFTFFALGFEHTVVNMFIIPTG
                     MLLGAKVSVYEWWVWNQIPVTLGNFVGGFVFTGLALYTTFKPVKPLALAASPIAAQVA
                     AE"
     gene            complement(14182..15351)
                     /locus_tag="VN968_00425"
     CDS             complement(14182..15351)
                     /locus_tag="VN968_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HXL27748.1"
                     /translation="MTGPLRIGFIPLVDAAALIVAIDKGFAAAEGLDVTLVREVSWSN
                     VRDKLNIGLFDAAHLLAPVAIASSLGLGHVKVPIVASFNLGLNGNAITVSPALHAAIM
                     SEIEGDPFDPMATALALARVIASRRQSGAEPLTFGMTFPFSTHNYQLRFWMAAGGVDP
                     DEDIRLVVLPPPYMVDSLANGHVDAFCVGAPWNSVAVDLGVGHILHFVADILVRAAEK
                     VLATRQEWSEKNPEILAALIRAHQRAAEFIEHSENRTETAGILAQPERIGVDADVIQR
                     TLAGRLKISPDGTMRESGRYLLVGREGAARPDPVQAAWLYAQMVRWGQATISPEAVKI
                     AKAVFRPDLYDAALGLRAGEDEAAFGSVGAFAGPAFDPDDVEGYLAAFNVFRRVP"
     gene            complement(15348..15938)
                     /locus_tag="VN968_00430"
     CDS             complement(15348..15938)
                     /locus_tag="VN968_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HXL27749.1"
                     /translation="MGAESSPKIVIVDESPIRAAILEEGLREAGFTGVVHISEMQSLL
                     SRIYALDPDVILIDLENPSRDVLEQMFQVSRAVRRPIAMFVDQSDSASIQASVDAGVS
                     AYIVDGLKKERIKPILDLCISRFNAFSKLQDELDRTKSALEDRKVIDRAKGILMKLKG
                     LTEDEAYVLLRSTAMREKKKIGEIAQSILTASELLK"
     gene            16050..17375
                     /gene="rimO"
                     /locus_tag="VN968_00435"
     CDS             16050..17375
                     /gene="rimO"
                     /locus_tag="VN968_00435"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HXL27750.1"
                     /translation="MQQAAAPKVSFVSLGCPKALVDSERIITRLRAEGYELARRHDGA
                     DLVIVNTCGFLDSAKRESLGAIGEAMAENGKVIVTGCMGAEPEQIEAAYPGVLSITGP
                     QQYESVLDAVHRALPPVHNPHLDLVPPHGVKLTPRHYAYLKISEGCNNRCSFCIIPKL
                     RGDLVSRPAGDVLREAEKLVAAGVKELLVISQDTSAYGVDLKYAASRWKDREVRARFF
                     DLAKELGEFGAWVRLQYVYPYPHVDEVIGLMTQGKILPYLDIPFQHASPEILKAMKRP
                     AAQDKTLARIGKWREECPELTLRSTFIVGFPGETDSDFAYLLDWLEEAEIDRVGCFKY
                     EPVAGAPSNALGHPVPEEIKQERWNALMARQQKISARRLKRKVGTRQQVIIDEVGPTV
                     SKGRSKADAPQIDGAVYLSSRRPLRTGEIVTAKIERADEYDLHGSIAGF"
     gene            17606..18802
                     /locus_tag="VN968_00440"
     CDS             17606..18802
                     /locus_tag="VN968_00440"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HXL27751.1"
                     /translation="MTNATHPFDALMDITTRPPTVFVRGEGSWLWDDSGKRYLDFIQG
                     WAVNCLGHSPPEVAEALAEQAKRLLTPSPAFYNAPSLKLAKALVDHSCFDQVFFANSG
                     AEANEGAIKLARRYGALHKNGAYEIITFEGAFHGRTLATMSASGKKAFEPLFEPKVPG
                     FPKAKLNDLASVKRLISDKTVGVMLEPIQGEAGVWPATDQFLRDLRSLTKAHGLLLIF
                     DEIQTGMGRTGTLFHYEHAGIEPDIMTLGKGIGGGVPLAALLATGAASCFEHGDQGGT
                     FNGNPLMCAAGLAVLDRVAAPDFLKSAVDSGLFLESELQRLSARHGLGEVRGRGLLLA
                     LDLKHPIGASIVAQAREDGVLLNSPQPDALRFMPALNVAREEIAAMIECLDAILTRMG
                     AARRVA"
     gene            complement(18967..19941)
                     /locus_tag="VN968_00445"
     CDS             complement(18967..19941)
                     /locus_tag="VN968_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HXL27752.1"
                     /translation="MTKAVRVHKVGGPEALVYEAVDVPAPGPGEVRIRQYAVGLNFID
                     VYYRTGLYKAPGLPFIAGNEAAGEVLAVGPGVTNFHPGDRVAYYFNLGGYASERNIPW
                     EKLVKLPDHITYEQGAVLMLKGLTVWYLLHKTFKIEPGHRVLIHAAAGGIGLLACQWA
                     KALGAHVIGTTGTRAKADLALANGCDHVILYNDEDFVARVKQISRNELCDVVYDGVGK
                     TTFPGSLSCIKPRGLFVSFGNASGPVPPFSIAELNNHGSLFATRPKLNDYIGKRSELL
                     EGADTLFAAVINGKLHVPINHAYALKDVARAHIDLESRATTGAAILRP"
     gene            complement(20033..20758)
                     /locus_tag="VN968_00450"
     CDS             complement(20033..20758)
                     /locus_tag="VN968_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HXL27753.1"
                     /translation="MIELLTSPEAWAALLTLTALEIVLGIDNVIFLSVIVSRIPQAQA
                     KRARQIGLALALVFRILLLSLLVWLIGLTQDVISLKGIGFSWRDIILIGGGLFLIGKA
                     THEIHAEVEARDEENDDAPRASAFFWIIIQIIVVDMVFSLDSIITAIGMAQDLAIMVA
                     AVVIACLIMYLSSGPVARFVGEHPTTKMLALAFLVLIGVALVADGFKFHIPRGYIYFA
                     IAFSAAVEAFNVLAKRNRKKVVR"
     gene            complement(20755..21486)
                     /locus_tag="VN968_00455"
     CDS             complement(20755..21486)
                     /locus_tag="VN968_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXL27754.1"
                     /translation="MNRENQQDSIISPATRATAFSVHIFTAMGAGVALVALLEAVREH
                     WAAMFGWLGVALVIDALDGPIARRLDVVRVQPNWSGEVLDLVVDFVTYVFVPGYAITA
                     SGLLLPVAAPLLGAGVVVSGALYFADRRMKAADNHFRGFPALWNAAAFYLFLLHLSPA
                     LSSLAVAALIVLTFVPVHVLHPVRVVRWRGLTLSLIGIWALLAIFTLASDFDVGAPVT
                     IGLCAIAAYIVGSDAVIRLARSFGA"
     gene            21610..22809
                     /locus_tag="VN968_00460"
     CDS             21610..22809
                     /locus_tag="VN968_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HXL27755.1"
                     /translation="MNEASVRYDVVVIGGGPAGLVAAIALAQTGAKMALVACRAPYAD
                     NRTTALLGGSVEFLEQLEVWPRCKDKAAALRTMRLVDDTGRLIRAPEVRFSCDEIGLE
                     LFGYNIDNRTLLTALEQRAAELSNLTRFDDEAETISPDDADVAIRTGDGKSLSACLVV
                     GADGRQSHCREAVGIDVRRRELNQAALTFNIGHSRPHRDISTEFHTPQGPCVFVPLPG
                     DRSSVVWVAAPKEAERLFALTDDELSEAAERQSHSILGRVHVEPGRHLFPLAIDQPRQ
                     FAKSRIALVGEAAHVLPPIGAQGLNLGLRDAADIADIVREAMLSGEDPGSPQVLKRYD
                     TARRADIASRTFAIDAANRSLLSDFLPMQSLRAAGLHLIGSFGPLRRLAMREGLAPSW
                     RPTRRAG"
     gene            complement(22978..23208)
                     /locus_tag="VN968_00465"
                     /pseudo
     CDS             complement(22978..23208)
                     /locus_tag="VN968_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
     gene            23332..23664
                     /gene="hspQ"
                     /locus_tag="VN968_00470"
     CDS             23332..23664
                     /gene="hspQ"
                     /locus_tag="VN968_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HXL27756.1"
                     /translation="MIKARTAKFQIGQIVRHRIFSFRGVIFDIDPVFNNTEEWWLSIP
                     EENRPHKDQPFYHLLAENSESEYVAYVSEQNLLPDDSGEPIRHSQVAEIFVKDKSGGY
                     RPRNPSLN"
     gene            complement(23968..24783)
                     /locus_tag="VN968_00475"
     CDS             complement(23968..24783)
                     /locus_tag="VN968_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HXL27757.1"
                     /translation="MNFRILAAPVWPRGRVFALLAATALAAASLAPDVQAQAPAPGAP
                     AAPKAKAAPKAAPKAAPAAPAPTAQQQQAPPAAGAAGAQPPDQQVQLIYAPWTKFCLK
                     GQDANAKQVCFTGKDGRIESGQPVIAAVIIEPEGEPKKILRVTLPLGMQLVHGTRVIV
                     DGNPPAQSPYVICFANGCMSDYEVTPELLANMKKGQNLVVQAINSNGAPLTLPLPLAE
                     FAKAYDGPPTDPKVFEETQKKLQEELQKRAEEARKRLEANPPNGAAAANPAAK"
     gene            25147..26889
                     /locus_tag="VN968_00480"
     CDS             25147..26889
                     /locus_tag="VN968_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXL27758.1"
                     /translation="MHGAPALPADFTHMPYANPDAPKGGRLVQGILGTFDSLNPLIVR
                     GVAVQQVVAVQQMRGVVIESLMARSNDEAFTLYGLLARSVETDDIRSYVTFHIDPRAR
                     FSDGQPVTAEDVLFSWALLRDKGRPHHRQYYSKVAKAEAPDPLTVRFDLGAANDRELP
                     LILGLMPILPKHAVDVATFEETTMTPLIGSGPYRVSAVRPGASVTLTRNPDYWGLNLP
                     VNRGLWNFDEIRLDFYREANGEFEAFKRGLYDFRVENEPLRWHDGYDFPAARNGEVIR
                     DTIKTGMPQPSEFLVFNTRRPMFSDIRVRQALTLLFDFEWINRNYFFGLYTRSAGFFA
                     GSELSAYGRAADDRERELLKPFVSHIPPDILNGSYRLPVSDGSGRDRVTLRSALNLLS
                     EAGYDLDGTVLRQRSTKAPLTFEILVTTRDQERIALAYTRDLKRAGIEAKVRAVDAVQ
                     FEQRRLGFDFDMIHNRWDQSLSPGNEQWFYWGSEAADHQGTRNYMGAKDPAIDALIAA
                     LLEAREHPAFVSAVRALDRALMSGFYAIPLFNVGEQWIARWNRIERPVTTALTGYLPE
                     TWWQNAGPSSKVIP"
     gene            26886..>27489
                     /locus_tag="VN968_00485"
     CDS             26886..>27489
                     /locus_tag="VN968_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HXL27759.1"
                     /translation="MIQTNASPTLDGLFRRILARKPDALALLDPLNKQRITGQPPKRL
                     TFAQADRAISALAAHFIEAGLPTNSVVAVQLPNTVEFMLTVLAAHRAGLIVALLPLLW
                     RQAELTVALNRTGARAIVTMGRVDGVVHADLAMNAAAESFSIRHVGGFGNDLPEGMVS
                     LDLALAGQSETTRAITQDGRRASIISFDVTVDGFRAVPRTH"
BASE COUNT         5306 a         8643 c         8456 g         5084 t
ORIGIN      
        1 cgaggatttt ggcatgctcg acggcctgcg tttcgatgcc gaactcggga ccaccgggga
       61 gagagcgggt ggcaacggtg ccgatggcaa gtatgtcgag cccaaggccc acatcatcat
      121 aacgacgcag acgattttgg cgcgttggct gcgcgcccac aaggactggt gggacaaggg
      181 cctcaagaat gtgccgcagc agatcgctgc agcgctcaag gatgaaagct tctacaccca
      241 ggcgatgtcg acggatgctg cggtggtgaa cttcgacccc ttgccgattg ccaagccggc
      301 gtccgccacc ttcgcctatg ggatgctggc ggcggcgatg ggaaggtagc gtgaagcctc
      361 gcgaactcgc gcgcggctac tcttgcggca cgtcttcacg cgcatgccgc tcatgggccc
      421 cgaccagtct cgaattcgtg gacctcgcgg ttcgccaaat cctggcctag ctcccctcac
      481 ccaccaacac gaagggcgcc caaatcgacg gatcggcgtt gcgaacggag gaccggtcag
      541 cgatcagcgc ttgcatggaa cgtcgtagag cttccgtccg gccaatatcc gggtgcttcc
      601 tcagctccgt gaaagcccca gtcgtcagcc tgactgcggc atgagaatct gccggccagt
      661 gtgacaccag cagcgagcgt gcacccgcat agaagaatgc gcgcgcgaga cctgaaaggc
      721 cttccgcttt ctgccatcac actccgcggg ccgtcacgac ggacattccg ccgtcggggt
      781 cactgctgcg atctcgggct tcctgtcgcc gccaaatgtc gtctcatgaa tcttgccgtc
      841 gacgatatgg atgcgccggt cggcccgcgc agccagtgcc gcgtcgtggg tcacgaccac
      901 cacggtctgg ccgccgttgg cgatatcgcg caggatgccg aacacctgct cggtcgaagc
      961 ggtgtcgaga tttccggtcg gctcgtcggc gacaatgatt tcgggacggt tggcgagcgc
     1021 ccgcgccagc gccacgcgtt gccgctgacc gccggaaagc tgattgggcc gcttgccggt
     1081 ctgctcgccg aggccgagcg acgtcagcag gctcatcgct ctctcgttca tatccttggt
     1141 cgacatccgg ccgtaagcgc gcattggcag cagtacgttt tcaagcgcgg tgaattcggg
     1201 aagcagaaag tggaactgaa acacgaagcc gcacttggtc agacggacat cggcgcgctc
     1261 ggtttccgac aatttggacg tggcctggcc gcaaatgatg acctcgcctt cggtaggtgc
     1321 atcgagcagg ccgagcaaat agagcagcga tgatttgccg gatcccgaag ggccggtgat
     1381 ggcgagaaat tcacccttcg ccacggatag atcgatacca tcgaccaggg tgtgagaaac
     1441 aatgccctga acgcggcgca ccagcttcac ggtttgcagg gcgacctcgg tcacgacgcg
     1501 ccccggataa tttccacggg atgcacccgc gtggccttgc gtgccggaaa gaacgccgcg
     1561 acggcgcagc agaaaatgga aatggctccg gtggcgagat aatgcgtcgg cgaatagtag
     1621 atctcgagtg gcaccgtcgc tccggtcagc acattgaata tcttgatctg agaccacccg
     1681 tagcacaaca gatatccgag aagccatccg aacaggattc cgaccgatcc catcatcgtc
     1741 gactcaatga tgaatattcg ccgcaccgag tattcccgca tgcccagcga tttcatgatt
     1801 gcgatgtcgt ggcgtttttc gtgagtgatg gtggaaatga tgttgtaggt cgcgaatgat
     1861 gagctcagca gcatggcgcc catcacggtc aggacaatga aatcacgggt agcgaaggcg
     1921 cccagcagat cggagttggc ctcctgccac gacaccgact tgtatccgat ttgtgcctcg
     1981 acgcgcgtgg ccacttgctg tgcgatcaag ggatcgctca ggcgcagccg caactgattg
     2041 acgagtccgt tctgtccgat gagagtctgg gccacattca tcaacgaata gatttgtcct
     2101 tcgtcgacgc ttttgattcc cgacttgaag atgccgacca ccgtggctga aaccggagct
     2161 ccgccgcccg tgccgagcaa gaccgtattt ccatatttca cggcaagttt ttccgccagc
     2221 gccgcgccaa tgatgatccc gttggccgcc tttgacagat cggtcagttg cccctcgagc
     2281 atctgcgagc cgagcttgga gacgcgcact tcaagccgcg gatcgatgcc gatcaggctg
     2341 acgccaaccc gcgcgtcgcc gtggttcaag atagccgtgg tctttaccga cggcgccacg
     2401 tcgcccggca accacgactt cagcgacgcc atgacggtgt ccggatattt gatcccgggg
     2461 cgccgattga cgtttgccac attgggcatg ccaaccgcag cgtattcctg gtccgccggc
     2521 tggctcggaa ccgaccggcg ttcatcacca accatgacat ggggcatggt gtcgacgagt
     2581 tgtcgcagaa aatcgatctg cgatccctgc atcaggccgg ccatcatgat ggtgaagccg
     2641 acacccatcg ccacgccaca catgccgacc agtgtttgtc gaatgcgagt ggtcacatga
     2701 gtccacgcga tactcagcag aagattcgtc gccatgggtt ttacctgtac ttgccgacgt
     2761 tcctgcgtgc atcgttcaca acggaatcga cgtgagccga tatggcggcg gagatgacgg
     2821 catcgtcggc atcggcagca ctggcaacca cggcggcggc gggcgcgcga tcaagcgcag
     2881 gactgggaac aggagcgata tctgcgggag cggcgggcgc cggcttggcg gccacgtttt
     2941 ccacgcggac ccgcgtgcca tcggccaggt cggcgcgtgc cggtgacagc acggtggtgt
     3001 ccttcgacac gtggccgatg atttcaacgt tgcggctgcc tcgaaccccg atggtgacgg
     3061 gcacccgctg aatttgtccg ccactgacga cctggaccgc gttgccggca acggcctcgg
     3121 caggcacgac gaccgccgcg gccttttcgc gatagatgat gttgacttcg accgtcattc
     3181 cgatccgcaa cggcgtatcg ggtggcaatc gcagatagac ccgaaaggtc tttcgcgtcg
     3241 gatcgccctt tggcgtgatt tgcgagaccg tggcccgcaa cgcctgcgcg gaaaacgcct
     3301 cgctgcgcaa aaaggccttc tgccccgcgg cgatcctggt gatttcctcc tcgtttattt
     3361 cggccaccac ctgcatcggc gtcggcgggc cgacccagaa cagcacctcg gttggtccga
     3421 caatctcgcc cacctcgccg tcgcgacgca acaccatgcc atcgagcggc gcgcgcagca
     3481 ccagcgaatc gatgcgggcc ttctgggcgc tcatccgcga tttggattgc tcaaactggg
     3541 tagagcgctg ctcgtattcg gtcttctggg cttcgctctt gtcacggtca cggctcgcgc
     3601 gatccagatc gcgcgcgagc tggtcattgt tgatttccag ttcatggagc gcgctgacct
     3661 cttcggcgtc gtcctggcgg ccgaggactt gaccggcttt gacggcctgc ccctcgcagc
     3721 ggcagagttc gaccagccga cgcctttgca gcggcacgac tttggcccag cgctcgggct
     3781 ccacggttcc cgtgccatag acggcctctg aaaccggccc gagaacagcc actcccgtgg
     3841 ttacggttgg aggttggaaa aagtccggat acttccaata ggccccgcca acggcggcaa
     3901 cggcaagcac tgcgagcact gatccggacc agacaagacc gcgcgaagac atcggcttct
     3961 cctaaaaata taaaatcaaa agccgaaccg cttgcgcagt ccggtgggca ctcgatccaa
     4021 tagcgcgtcc atatcgttct tcctttcgct ggtgttgcgc ggccctggaa gactgccggt
     4081 aacgctggaa ttgttactcg tcgtcgcggg cggctgtttc gaaacagccg gggatagttg
     4141 cggcgaaggc tggtagtcgc gggtgagact gtcggccgga gccggagcaa cggcaggttt
     4201 cggctggctc gatgtcgcgt gccagcggcc ggcttcggcc ttggcccgct caacatcgtc
     4261 gcgcgtcatc ctcgtttcca gaacgcccat gagctggcga cttccattgc ggaattcgtc
     4321 aacccggctg ccaaccgcga caatgtaggc ccacttataa gcgttgaagt tgtctttcga
     4381 aattcctcgc ccaacggcat acatgccgct gagaaccagc atggcatgcg aatgaccctg
     4441 gtcggcggcg cgcgaaaacc atcggaaggc ctcttcatcc gacctcgggc gccccacacc
     4501 cgacgagtaa agcgcagcga gatagaattg agcgtcgcta tctcctgaca gcgccgcctg
     4561 tgaatagcgg tcggccattt ccttgtcgga taccgcatcc ccggcatggg cgacggagct
     4621 cgcgatgaga gcggctccgc aaagcattgc tagaaaaact cgcttcatgg aaatccccgt
     4681 ccaaaataac ccagcgcgtt atttcgctga tgttacccgt gatcgtttct tagcccaagg
     4741 ccttatctcc tcatgtgatt tctctcacag tctccgacga agcgatctga gaggttgatt
     4801 gcgccggaac caagggagca tgtcatggga tacttctgca aaacattcgt cgccacccgc
     4861 atcctgatac tgctgggaac ggtggtcgcc acgcccgttg cggcctcgac atttgacggg
     4921 gcatggaacg tccagattgc ctcggcgaat gccgcatgcg gcggggcgac catttcgatt
     4981 ggaatcgcca atggccaggt cgaatccagc aatgcattga tgaaagcgac cggccgggtc
     5041 gccgatgccg gtggcatcag cgtaaccctg acaagcggaa tcaagcgggc tgtcggctcg
     5101 ggtcacctca gcgcgacgtc gggatcgggt acctggcgcg gcgcgctgtg ctcgggtacc
     5161 tggacagcgc agcgtatcta gccgccccct ctggcgtcat ccgaagatac gagccagccg
     5221 cggcgacact cggaggacga cgaccgacct cccgtcaacc tacgggagcg ccatattccg
     5281 cgtccgtcac gggctccatc caggtcgcat gatttccgtc cagcgattcc tgcatcgcga
     5341 tgtggatcat gccgttggtc ggcgagccgc cgtgccaatg tttttcgccc ggcggaatcc
     5401 agacgacatc gccagccctg atctcgcgta tcggcccgcc tttgctctgg acccggccga
     5461 cgcctgagat cacgtacagc gtctggccga gcgggtgcga atgccaattc gtccgcgcgc
     5521 ccggctcaaa ggatacgcgg ttcgagacaa tccgcgccgg tacctcggcc gcaatgatcg
     5581 gatcctgcca gaccgtgccg gtaaaatatt ccttgggcgc gaggcgtgtc ggccgcgagc
     5641 cggcgagatg gatattcatg gcattcctcc ttgttatgca gacgcttccg gtacgttctg
     5701 gctatttctt cgaggccgcg tagcgcgcct tggtctcggc attcatggga tagagcccgg
     5761 gcaacacggc gccgccattc acctgttcga cgatccaggc ctccattcgc tcctgctccg
     5821 cgccttcggc cagaatgagg tcgagaaacg ctttcggaat caccacggct ccgtcctgat
     5881 cggcgacgat aatgtcgtcc gggaagatcg caacgccgcc gcagccgatc ggctcacccc
     5941 aaccgacgaa tgtcagtccc gccaccgagg gtggggcggc aaagccgtcg caccacaccg
     6001 gcaagccggt gccgagcacg ccttcgagat cgcgtacgac gccgtcggtc accagcgcag
     6061 ccaccccgcg tttgaccatc cgcgcacaca gaatatcgcc gaagatgccg gcgtcggtga
     6121 tgcccatggc gtcgactacg gcgatgcagc cttcgggcat ggcttcgatc gcggtccggg
     6181 tcgaaatcgg cgacgaccat gattccggcg tggccagatc ctcccgcgcc ggcacgaaac
     6241 gcaaggtaaa ggccggtccg accagccgct tctgtcccgg ccgcagcggg cgcgaaccgc
     6301 gcagccagac attccgcagg cccttcttga gcaaaaccgt tgtgatggtg gcggtggaca
     6361 cgtgcgaaag ggtcttgatc gcttcggggg ttagggacat cgaaaaagta gctccggttg
     6421 ctgattgatt gggcgttgcg catcttgcgg ggcaagcaca tcgcgtcaag tgtcgggaac
     6481 tcacggctga aggcgcggca ccgcatggcg cgatcaggcg gtcttatcac caggatacag
     6541 attaatttat tgaacttgca ggccgatttc cgcgaagcga attccacatc gcggcaaaac
     6601 aggctagacc ttgatactcc cgccgacgag tttgtgaggt tatggccgcg acacgatccc
     6661 cgccccgcct gttgccgagt ggcgacagcg ccatcacggt agaattcagc cgcagcatcg
     6721 atgaaatggc gaaccagcgg gtgctggcgc tcgatcgcac ccttgcacgc gagaccatca
     6781 ccggcgtaac cgagaccgtg ccgacctacc gatcccttct ggtgcattat gacccggtcc
     6841 agatcgactt cgacacgctg agcgggaagc tcgtcaccct cgcgctgttg ccggttccac
     6901 cggttacgaa aatgcggcgc tggcgcattc ccgtggctta tggcggcgag catggcatcg
     6961 atctcgaaga cgtcgccagg acactcaagt cgacgcccga aaacatcgtc gcgcggcatg
     7021 tcgccggcga ctaccgcgtc gccatgatcg gctttacgcc gggctggtcc tatctcagcg
     7081 gccttgacgc ctcgctgcag atgccgcggc tgcaaaatcc gcggctggtg acacccgccg
     7141 gcaccgtctc gatcggcggc gtgcagaccg gcgtgcaatg tctcgccggt ccgagcggct
     7201 ggcatctgct gggccggacc gcggtgcgaa cctaccagtt gcatcgcgat ccgattttcc
     7261 tgctggaacc cggcgatgcc gtgacgttct ccgcggtcga cgccaagact ttcgccgagc
     7321 aggatcgcgc cgcggcggcc ggcgagatcg ttgccgagtt gatgacctca tgagcaaact
     7381 cgctatttcg gcgattggcc cggcaagctc ggtgcaggat ggcggccgtt acggcgccca
     7441 gcgttacggc ctgacgccga gcggcgcgat ggaccggctt gcgctcgctg cggcgaatag
     7501 cctggtcgga aatgcaactt tcgccgccgc gattgaaatc ggcccgttcg gcgccaaatt
     7561 cacggcgcgg gagggtgccg ttcgcattgc gctcgcgggc gcatcgcgca acgccgacat
     7621 ctcgggacgc gcggttgtat cgaatacatc cacgacgatg gccgacggcg aaacgctgac
     7681 gctgggtttc gcccgcggcg gctctttcag ctaccttgcg atcgaaggcg ggattgtggg
     7741 cgaaccgatg ttcggcagcc tggcagtgaa cgcgcgcgcg gggctcggca gcccgtatcc
     7801 acgcccgctg caggccggcg acgaattgca gacggcggcc gcaagcggcg cggcggagaa
     7861 ccggatcgat cttccacccc ccgcggacgg gccgatccgg gtggtgatgg gtccgcagga
     7921 tgacgagttc ggggatgcca aagcgttatt tctcgaccgc gaatggaaga tatcgtcggc
     7981 cagcgaccgc atgggttacc gccttgaagg cccggtgatc aaacatctgc acggtcacaa
     8041 catcgtctcc gacggtaccg tgaacggcag tgtccaggtg ccgggcaatg gccagccgat
     8101 cgtgctgatg ccagaccgcg gcaccagcgg cggctatccc aagatcgcca ccctgattac
     8161 cgccgatctt ggccggttcg cgcagattcc ggcaggaacc ggctttcgtt tcagggccgt
     8221 cagcatggcc gaggcgcagg cggaggcacg gaagtcttcg gaaatgctgc gcaccctgcc
     8281 cgaccgcctc agaccgatcg acaattttgc gctcaacatc gaggcgctgc gagatgctaa
     8341 cgtcgccggc gccgcggtca gtgcagtcga cgccggaacg tggcaaattc catcgtcggc
     8401 ggatgcgccg gcgcaagact ggttgacgca gacgcaagac caaccaaagc tccactcacg
     8461 gcaagcggac acgacatcat gacaatcgat ctgaattgcg atctcggcga agggttcggc
     8521 gcatgggaaa tgggcaatga cgccgccatg atcgacctcg ccacctcggt caacatcgcc
     8581 tgcggcttcc atgccggcga ttccgacacc atgcgcaaga ccgtggaact ggcgaaagcg
     8641 cgcggcgtca gcatcggcgc gcatcccggc taccgcgacc tgcacggctt tggccgccgg
     8701 ccttttccgg gcatgaaggc atccgagatc gaaaacctgg tcgcctacca gatcggcgca
     8761 ttgcaggcga tcgcgaccat ggccggccac aaggtgaccc acgtcaaggc gcatggcgcg
     8821 ctgtcgaatg ttgcctgcga ggatgaggcg accgcgcgcg cgattgcgtc cgcgatcagg
     8881 gcggtcgatc ccgatctgat ttttgtggtg ctcgccaatt cgaagctggt gcaggccggc
     8941 gaagccgcca acctgccgat ggtgcacgag gtgttcgccg accgcgctta tgaagacgat
     9001 ggctcgctgg tgtcacgccg caagccgggc gcggtgttgc acgatgccag ggagatcgca
     9061 cagcgcgtgg tgcggatggt gcaggacagc gccgtggtgt cggtcaccgg caaggtgatc
     9121 aagatgcgca ccgacaccgt atgcattcac ggtgacacgc ccggcgcggt cgatatcgcg
     9181 cgcggtgtgc ggcaggcgtt gaaagatgga ggaatcgaag tcgcgccgtt caagaaagca
     9241 agttagcgtc cgcgcctaga aggggtgaac cgacagcgtg ccgaacacca cggccgcaat
     9301 gacggcgaag gcgccataaa gggcggcggt atgcaaacct gtccctgtgc gcttcgaaag
     9361 cgaccgcgca taaagatgga gccgggcttc cgccgataat tcgcgcatag tcgatctcca
     9421 acgccgcatc gccacataat ggagtaccga ttgcggcggg attcaagacg accttgcaaa
     9481 tagcgacagc aacgctttga ccgactccga atgcagtcaa aaattctctc cagcggattc
     9541 aaacgagaat tttattcgtt cggattgtag ccatctggaa cccgctaata gacatcctgc
     9601 tggaaccggc ctttcttttt gagttccgca acgaagctga ccgcctcgtc ggtcgagcgc
     9661 gcaccgaact gcgcgacgat atcgaccagc gcgcgctcga catctttcgc catccttttg
     9721 gcatcgccgc agatatagat gttggcgcct tcggcgagcc acgtccataa ttcacggcca
     9781 acctcgcgca tgcggtcctg cacatagaat ttcttgtcgc cgtcacgcga ccacgccagc
     9841 gacattcgcg tcagcagccc tgacgttttc atggcgttca actcgtcggc atagaagaaa
     9901 tcgcaactgc tgcgctgatg gccgaagaac agccagttct tgcccggcgc accggtggct
     9961 cggcggtcga gcaggaaggc acggaacggc gcgacaccgg tgccgggccc gatcatgatg
    10021 atcggcgtct tcggatcctg cggcagcgca aaaccgtggg ccttctgcac atagactttc
    10081 agctgatcgc ccggcttgat gcgttcggcc agaaatgtcg aggccagtcc gaggcgcttg
    10141 cgcttgccga cgacatagcg cacgcagtcc accgtcagcg acagcttgcc gggcgtggcg
    10201 ttgtgcgacg acgagatcga atagagccgc ggctgcagcg gctccagcgc ttcgacgaac
    10261 gcttcgggat gcggacgcac gccggaaaat ttttgcagcg ctgccatcac atccagcgtc
    10321 gcggcgtcgc catcgggatc ctcaccttgc gccagcgcgc gcgccttctc gcgctgcgcg
    10381 ccgccggtga tgaacgaaat cagttcgaac agcgaatccg gagcaggcgc cagcgagaca
    10441 tcgtcgatca agacctcacg cagggtcttg cctcggacct ccgtcgtatg cgaggcgccg
    10501 agcaaggcga tgatctgatc gacgtgaccc agatcgttgc gcgcaaagat gccaaaggag
    10561 tcgccgacga cgtagtcgag gccgcaaccg ctgagatcga aatcgatgtg ccaggtttcc
    10621 ttttccgatc cgctttcgtt cagcaagcgg cgcgatacga aggtcgccgg caccggattg
    10681 tcgcgcgaac gtccggggtc gaccgcgatc gcgggcgccg ctgccgcggg taccgctgcc
    10741 acccccgaca aagccggagg cgccttctcc agttcctcgt tcaaggcctt gagcatccgg
    10801 gcggtttcct ttcccccggg gacgcagagg ttgagccgcg gttcggcctt gctgacgatc
    10861 gcatccgaat agtcgttgca attgtagccg cactggccgc aatcctgctg cgccatcgcc
    10921 gccatcatgc ggcgccgcaa ggggcggcct tcggcaagct tcatgcggtc ggcaatcggc
    10981 atggtctggt cgtgccacgg cgcttcgcca tcatcgccat cgccggacgg cgcaagcaac
    11041 acgccgttct gcgccggcgc cagcggtgtt gaaccgtcga actgcagcag cccggcgagg
    11101 aaaccgttca gccacgaacg ctgtccctcg ctaaacggtg cagttggcgg aatgatttca
    11161 agccgtggtg gtggggtcat ctgattcatg tcattgcttc cgcttcagcc agcttgcgca
    11221 aggcttcgcc gtcatggcgg cgtgaaaatg tgagaaacgt ctcctcagcc gagacacggt
    11281 tggcaagata ggccttcaat aatttttcca ccgtcttcgg cgcatcctcg gccttcacgt
    11341 tgcgatagac ctcctgcccg atatcggcat ccggtccgaa accgccgccg gcataaaggt
    11401 ggtaaccctc gaccgtgtcg tcgtcgcttc ccgccggcac ctttgccgcg agcagcccga
    11461 tatcgctgat gtaatgctgg gcgcaggaat gatgacagcc ggtgagatgg atattcagcg
    11521 gcgtttcgat tgacacgcgt gtctcgcacc agtcgccgat ctcggcggcg tggcgtttgg
    11581 tatcggaggc tgcgaacttg cagccggcat tgccggtgca ggcaatcagc ccggcgcgca
    11641 tctgtgacgc cttgaccgcg agcccgagcg tctcgatcgc agccgtcgcc agcgccacgt
    11701 tctcgtcgcg cacacccgat atcagcagat tctgccacac cgtgaggcgg atatcgccgt
    11761 cgccgagatc ccgcgcgatt ttggcaaggc cgcgcatttg atcgcaggtc agtttgccca
    11821 ccggcagcac gacgccgatc cagttcaggc ccggctgctt ctgggcatgg ataccgatat
    11881 gggccatccg atcgaatgcc gggcgcagcg cgagagcttc cggcggcacc cgggtcaagg
    11941 cgtgacccag cttttcctcg accagaacga ggaatttttc catgcccatg ccgtcgatga
    12001 catatttcaa ccgcgccttg agccggttgg tacggtcgcc aagatcgatg aacacgcgga
    12061 cgatggcgtc cgccactttg gtggcatccg ccggtttgac gatgatgccg gtctccttgg
    12121 caaaatcctt gtgaccggtg atgcctccaa taccgaggcg aaaccatacg ccgggatcga
    12181 ccccgaagcc atccttcacc tcgaccgcgg cgaaggcgat gtcgttggtg tcctcaagca
    12241 ccgcgattct gccggcgccg tcgaaggcga cattgaactt gcgcggcaat ccggtcagcg
    12301 agcggtcgtt gagaatgtga tagtgccatt gccgggcgta ttcgcgggtg tcgatcaatt
    12361 cctgcggatc gatcccggcg gtcggcgtgc ccgtgacatt gcggatatta tcggcgccgg
    12421 agccgcgcga gcacaggccg agatcctgga tgccttcgat gagcgccacc gtatgcttcg
    12481 gcggaatctc gcgcacctga agattggcgc gggtggtgac atggcaaaac ggtccgcaca
    12541 ggcgttcggc gagatcggcc atccctgcga actgccagtg cttgaggatg ccgttcggga
    12601 tccgcaaccg gcacatgtag gaatcctgcg ccggcgcgac ataaaacagg ccgtaatagc
    12661 gccagcgaaa attatccgcc ggcgttggcg gcgcgttggt cacagcctgt tgcttcagcc
    12721 gcgaataagc atcgaatgga tgctcttcgc gtttgaactt ttcctggtcc gcaagctttt
    12781 tgcccgacgc gatgaccttg tcctgggctt tgaagcccgc ggcgtccgga cctatcggct
    12841 ccggagcggc cttgccggcc ggcacggcgc caccggcgag gcgccctacg cgccccacct
    12901 gcaggcccga catgaagcct tccagatagc gcttctgctc gtcgctaaaa tcggcggttg
    12961 tggtttcaag tttcatgatg cgccgccgtt attctgccgc gacttgcgct gcaatcggcg
    13021 atgccgccag cgccagcggt ttcaccggct tgaaggtcgt gtagagggcc aaaccggtga
    13081 acacgaagcc gccgacgaaa ttaccgagcg tcaccggtat ctgattccac acccaccact
    13141 cgtaaacgct caccttggcg ccaagcagca tgccggtcgg aataatgaac atgttgacga
    13201 cggtgtgctc gaatcccagc gcgaagaagg tgaagatcgg cagccaggtc gcggcgatct
    13261 ttcccacggt ggaagtcgac gtcatcgcca tgaccacgcc gagacaaacc agccagttgc
    13321 agaggatggc tttgacgaag acactgacca ttccggccgc gccgagcgcc tcgttggcga
    13381 cggtttttgc ctcggcaatg ctgatgacgc gcgccgcgac cccggccggt tcgatcttcc
    13441 ccatattggt gaggctaatc gcaagaaggg cgccgaaggc gatgctgcca agcaggtttc
    13501 caagaaacac ccacgaccag ttcgcgatga tggcgttcca ggtggccttg ccctcgagtc
    13561 gcgccagcgg cagcagcccg aaacttccgg tcacgagttc gagcccgagc agcacgatca
    13621 tcacgagact gacgggaaaa atcagtgcgc cgaccagcgg ctgacccgtc gtgatggcac
    13681 cactgaatgc aagcgcggtg gcagcaccca gcagcgcacc ggaaagcgcg ccgcgaatga
    13741 gcagatcccg aggcgaaagc gccagtttcc tcaaactggc atcgatcatc gaggcgacaa
    13801 cgtcggcagg ttttgcgtaa tccatatcga actccagcgc cgcgcgcgtg cggctgcaac
    13861 gaggaaaggg gcatgcgatc gcttggaaat tcgatccgaa gggcagcgcc gtatcggaga
    13921 tttcgaagcg atccgatcag cctcgttgct gcggaaatcc atcttggacg acgccagcgg
    13981 gcatctacgg cggctggcgt gctctgctcc attcaaatag tgtgccagtg cagcaattct
    14041 tggaaagctc ttctcgccca atggattact gatattgcga cgcaatataa gggttaaccc
    14101 agctgccgca taaggctgca ggcgcctaaa acataaacaa ggaaaaaatc gcctaatatt
    14161 taatcggggc gttgttccac tttatggaac tcggcgaaaa acgttgaatg cggcgaggta
    14221 tccctcgacg tcatctggat cgaatgcagg ccctgcgaac gcgccgacag aaccgaatgc
    14281 ggcttcgtcc tcgcccgcgc gcagtcccaa agccgcgtcg tagagatccg gcctgaacac
    14341 tgccttggcg atcttgacgg cctcggggct gatcgtggcc tgcccccagc ggaccatctg
    14401 cgcataaagc caggcggcct gcacgggatc gggccgggcc gcgccctccc gccccaccag
    14461 cagatagcgg ccgctttcgc gcatggtgcc gtcgggggaa atcttcaggc ggccagcgag
    14521 cgtccgttgg ataacgtcgg catcgacgcc aatgcgttcg ggctgggcca gaataccggc
    14581 ggtctcggtc cgattttccg aatgctcgat gaactcggcg gcgcgctgat gcgcgcggat
    14641 caacgccgcc aggatctccg gatttttttc cgaccattcc tgccgggtcg caagcacctt
    14701 ctcggcggcg cgtaccagaa tatccgcgac gaaatgcagg atatgtccca cgccgagatc
    14761 gaccgcgacg gaattccagg gcgcgccgac gcagaacgca tcgacgtgac cattggcaag
    14821 gctgtcgacc atgtagggcg gcggcagtac caccagacga atgtcctcgt cgggatcgac
    14881 cccgcccgcg gccatccaga accgcaattg ataattgtgg gtcgagaacg gaaaggtcat
    14941 gccgaaggtc agtggctcgg cgccgctctg gcgccgcgac gcgatgaccc gcgccagcgc
    15001 cagcgccgtc gccatcggat cgaacggatc gccttcgatc tcgctcatga tcgccgcatg
    15061 cagcgccggc gataccgtga tggcattgcc gttgaggcca agattgaagg aggcgacgat
    15121 cggcaccttg acgtggccga gcccgaggct ggacgcgatc gcgaccggcg ccagcaggtg
    15181 ggcggcgtcg aacaggccga tgttgagctt gtcgcggaca ttcgaccacg acacctcccg
    15241 caccagtgtg acgtcgagcc cctccgcggc ggcgaatccc ttgtcgatgg cgacgatcag
    15301 cgccgcggca tcgaccagcg gaatgaagcc tatgcgaagc ggtccggtca tttcagcaac
    15361 tccgaggcgg tcagaatcga ctgcgcgatc tcgccgatct tcttcttctc gcgcatcgcc
    15421 gtcgatcgca acaaaacata ggcctcgtct tcggtcaggc ctttcagttt catgagaatt
    15481 cccttggcgc gatcgatcac cttgcggtct tcgagcgcgg atttggtgcg atcgagttcg
    15541 tcctgcagct tcgagaaggc gttaaagcgc gagatgcaga gatcaaggat cggcttgatg
    15601 cgttctttct tcagcccgtc gacgatatag gcagagacgc cggcatcgac cgaagcctgg
    15661 atggatgccg agtcgctctg atcgacgaac atcgcgatcg gccgccgcac ggcgcggctg
    15721 acctggaaca tctgctccag aacgtcgcgg ctggggtttt ccagatcaat caggataacg
    15781 tcgggatcga gcgcatagat ccggctcagc aaactctgca tttcgctgat gtggaccacg
    15841 ccggtaaaac ccgcctcccg cagcccctcc tcgaggatcg cggcacggat cgggctttcg
    15901 tcgacaatga caattttggg agacgactcg gcgcccatcg atcactcgca ttccggcaaa
    15961 acatccatag cacgccgcaa gcccgctcaa agccttgtaa ttatgggcaa ttgggactag
    16021 ctcctgccaa tcaatcaggc agttcaaata tgcaacaggc cgccgcgccc aaagtcagct
    16081 ttgtttcact gggatgtccc aaggctttgg tggattccga gcgcatcatc acccggctgc
    16141 gcgcggaggg ctatgaactg gcgcgcaggc atgacggtgc cgatctcgtc atcgtcaaca
    16201 cctgcggctt tctcgacagc gccaagcggg agtccttggg cgcgatcggc gaagccatgg
    16261 ccgagaatgg caaggtgatc gtcaccggct gcatgggcgc cgagcccgag cagatcgagg
    16321 cggcctatcc cggggtgctg tcgatcaccg gaccgcagca atatgaaagc gtgctcgacg
    16381 ccgtgcatcg cgcgttgccg ccggtgcata atccgcatct cgacctggtg ccgccgcacg
    16441 gcgtcaagct gacgccacgg cattacgctt atttgaagat ttccgagggc tgcaacaacc
    16501 gctgcagttt ttgcatcatc ccgaaactgc gcggcgatct ggtgtcgcgt ccggcgggcg
    16561 atgtgctgcg cgaagccgaa aaactggtag ccgccggggt gaaggaactg ctggtcattt
    16621 cgcaggacac gtcagcttac ggcgtcgatt tgaagtatgc ggcgagccgc tggaaggatc
    16681 gcgaggttcg ggctcggttt tttgacctgg caaaggagct cggcgaattc ggcgcctggg
    16741 tacggttgca atatgtctac ccctacccgc atgtcgatga agtcatcgga ttaatgacgc
    16801 agggcaagat cctgccctat ctcgatattc cctttcagca cgccagtccg gaaatcctta
    16861 aggccatgaa gcgcccggcg gcgcaggaca agacgctggc gcggatcgga aagtggcgcg
    16921 aggaatgccc cgaactgacg ctgcgctcca ctttcatcgt cggctttccc ggcgaaaccg
    16981 attccgattt tgcctatctg ctcgactggc ttgaagaggc cgagatcgat cgcgtcggct
    17041 gcttcaaata tgagccggtc gccggtgcgc cgtcgaatgc gctcggtcat cccgtgccgg
    17101 aggagatcaa gcaggaacgc tggaacgcgc tgatggcgcg gcagcaaaaa atttccgcgc
    17161 gccggcttaa gcgcaaggtc ggcacgcggc agcaggtcat catcgacgag gtcggtccga
    17221 ccgtctcgaa gggccgatcc aaagccgatg cgccacagat cgatggcgcg gtgtatctgt
    17281 cgagtcgccg accgcttcga accggcgaaa tcgtcaccgc gaaaatcgaa cgcgccgacg
    17341 aatacgacct gcacggcagc attgccggct tctgacgctc ttgacagcgc cggccgttcg
    17401 gctgtatggc ggcaccatga aactcaaccg gacccaactc cgcgccactt tccgtcgtcg
    17461 ctcgttcgac gatgatgggt cgcgccgtgt ccgacgacaa cgctgaacag atagatcagc
    17521 aacgttttcg agaaggccgc accctcaaaa gtgcggcttt ctcgcgtttc gcactatttt
    17581 tcatcgcaac ccagaggagt tcgacatgac caatgctacc catccgttcg acgcgctgat
    17641 ggacatcacg acgcgcccgc cgaccgtatt cgtgcgcggc gaaggctcgt ggctgtggga
    17701 cgacagcggc aagcgctatc ttgacttcat ccagggttgg gcggtcaatt gcctcggcca
    17761 ttcgccgcct gaagtcgccg aagcgctcgc cgagcaggca aaacggctgc tgacgccgag
    17821 cccggcgttt tacaacgccc ccagcctcaa gctcgccaag gcgctggtcg atcacagctg
    17881 cttcgaccag gtgttctttg ccaattccgg cgcggaagcc aatgaaggcg cgataaaact
    17941 cgcgcgcaga tacggcgccc tgcacaagaa cggcgcttac gagatcatca ccttcgaggg
    18001 cgcatttcat ggccggacgc tggcaacgat gtcggcctcg ggcaagaagg cctttgagcc
    18061 gctgttcgag ccgaaggtgc caggctttcc caaggccaag ctgaacgatc tcgcctcggt
    18121 aaagcggctg atctccgaca agaccgtcgg cgtgatgctg gagccgatcc agggcgaagc
    18181 cggcgtctgg cctgcgactg atcaattcct gcgcgatctg cggagcctga caaaagccca
    18241 tggcctgctg ctgatcttcg acgagatcca gaccggcatg ggccggaccg gaacactttt
    18301 ccattacgag catgcgggaa tcgagcccga catcatgacg ctgggcaagg gaatcggcgg
    18361 cggcgtgcca ctggccgcat tgctggccac tggagcggcg tcctgtttcg aacatggcga
    18421 ccagggcggc accttcaacg gcaatccctt aatgtgcgcg gccgggcttg ccgttctcga
    18481 tcgggtcgca gccccggact ttttgaaatc ggccgtcgat agcgggctgt ttctggagag
    18541 cgaactgcag cggctttccg cgcggcacgg tctcggcgag gtccgcggcc gagggctgct
    18601 gctagcgctc gacttgaaac atcccatcgg cgcgtcgatc gttgcgcaag cgcgtgaaga
    18661 tggcgtgctg ctcaactcgc cgcagccgga cgcgctgcgt ttcatgcctg cgctcaacgt
    18721 cgcacgggaa gaaatcgccg caatgatcga atgcctggat gcgatcctga ccaggatggg
    18781 cgcggcaagg cgtgtggcgt aagtgagcgc accgacgtca tccccgcgta acggcttcgc
    18841 cggtatcgct ggtggagccg cgctcgtgcg ctgcgtctcg aaggatggat cacagctctt
    18901 gtcatgcttc gaggctcgcc gaagacggcg agcacctcag gatgacgaca gtgtgcgatg
    18961 gagtctttac ggccgcaaga tcgcagcccc cgtcgtcgcg cggctctcga gatcgatatg
    19021 ggccctggcg acatccttca gcgcgtaggc gtggttgatc ggcacgtgca gcttgccgtt
    19081 gatgacggca gcaaacaacg tgtcggcgcc ttccagcagt tccgagcgct tgccgatgta
    19141 gtcgttgagt ttcggccggg tcgcaaacag cgagccatgg ttgttgagtt cggcaatcga
    19201 gaacggcggc accggaccgg aggcgttgcc gaaactcacg aacaacccgc gcggcttgat
    19261 gcaggacagc gaacccggaa acgtcgtctt gccgactccg tcatacacca cgtcgcaaag
    19321 ctcgttgcgg ctgatctgtt tgacgcgcgc gacgaaatcc tcgtcgttgt agaggatcac
    19381 gtgatcgcag ccattggcga gcgcgaggtc ggccttcgcc cgggtgccgg tggtgccgat
    19441 gacatgggcg cccagcgcct tggcccattg gcaggcgagc aaaccgatgc cgccggccgc
    19501 ggcgtgaatc aggacccggt gtcccggctc gatcttgaag gtcttgtgca ggaggtacca
    19561 gaccgtcagc cccttcagca tcaggacggc gccctgctcg taggtgatgt ggtcgggcag
    19621 tttgacgagc ttctcccagg ggatgttgcg ttccgaggca tagccgccga gattgaaata
    19681 ataggcgacg cggtcgccgg gatgaaaatt ggtcacgccg ggcccgaccg ccaaaacctc
    19741 gccggcggct tcattgccgg cgatgaacgg cagcccgggc gccttgtaga ggccggtgcg
    19801 atagtaaacg tcgatgaagt tcaggccgac ggcatactgg cggatgcgta cctcgccggg
    19861 tcccggcgcg ggcacgtcca cggcttcata aaccagggct tccgggcccc ccaccttgtg
    19921 cacgcgcacg gccttggtca tctcaggttc tccgctggca gtcccttgaa atcaaacgaa
    19981 agctatcgag gctgcgttgt caactcaacc gccatgggaa cgtctccgcc gcctacctga
    20041 ccaccttttt gcggttgcgt ttggccagga cgttaaaggc ttcaacggcg gccgaaaatg
    20101 cgatcgcaaa atagatgtag ccgcgcggga tatgaaactt gaatccgtcg gcgaccagcg
    20161 caaccccgat cagcaccagg aaggccagcg ccagcatctt ggtggtcgga tgctcgccca
    20221 cgaatctcgc gaccggaccg gacgacagat acatgatgag acaggcgatg acgaccgccg
    20281 cgaccatgat ggcgaggtcc tgcgccatac cgatcgcggt gatgatggaa tccagcgaga
    20341 acaccatatc gacgacgatg atctggatga tgatccagaa gaacgcgctg gcgcgcggag
    20401 catcatcatt ttcttcgtcg cgcgcttcca cttccgcgtg aatttcgtgg gtcgccttcc
    20461 cgatcaggaa caggccgccg ccgatcagga tgatatcgcg ccacgaaaag ccaattccct
    20521 tcagggaaat gacgtcttgc gtcaggccga tcaaccatac caggaggctc agcagcagga
    20581 tgcggaacac cagcgccagc gccagcccga tttgccgcgc gcgcttcgcc tgcgcctgcg
    20641 gaatccgcga aacgatcacc gacagaaaaa taacattgtc gatgccgaga acgatttcca
    20701 gcgcggtcaa ggtcaacaat gcggcccagg cttccgggct ggttagcagt tcgatcatgc
    20761 accgaacgat cttgcgagcc ggatcaccgc atcgcttccg acgatatagg ccgcgatcgc
    20821 acagagtccg atcgtgacgg gcgcgcccac gtcgaaatcg cttgccagcg tgaagatcgc
    20881 aagcagcgcc cagatgccga tcagcgacag ggtgagcccg cgccagcgca caacccgcac
    20941 cgggtgaagg acatggaccg gcacaaatgt cagcacgatc agcgccgcga ctgcaaggct
    21001 cgaaagcgcg gggctcaaat gcagcaggaa cagatagaat gccgcggcat tccacagcgc
    21061 cgggaatccg cggaaatgat tgtccgcggc cttcatccga cgatccgcga aatagagcgc
    21121 gcccgacacc acgactccgg cgccgagaag cggcgcggcc accggcagca gcaggccgct
    21181 tgcggtgatg gcatagcccg gcacaaagac gtaggtgacg aagtcgacca ccagatcgag
    21241 cacttcgccc gaccagttcg gttgcacgcg cacgacgtcc agccgccgcg cgatcgggcc
    21301 atcgagcgcg tcgatcacca gcgcgacccc aagccagcca aacatcgcgg cccagtgctc
    21361 gcgcaccgcc tccagcagcg cgaccagggc gacgcctgcc cccatcgcgg tgaagatatg
    21421 cacggaaaag gcggtggccc gcgttgcggg cgatatgatc gaatcctgct ggttttcccg
    21481 attcatttgc ccgcactgct atcaggaatc gccccgattt gcacatccgc gcttgcggcg
    21541 ttccgccacg tagatttggt agcgtgaacc gggcttgaaa acgccgccgg gaacgtcaat
    21601 tgtcccgata tgaacgaggc atcagtgaga tacgacgtcg tcgtcatcgg cggcggcccg
    21661 gccggacttg tggcggcgat cgcgttggcc caaaccggcg cgaaaatggc tttggtcgcc
    21721 tgccgggccc cctatgccga taatcgcacc accgccctgc ttggcggctc ggttgaattc
    21781 ctggaacaat tggaggtttg gccgcgctgc aaggacaagg cggcggcgct gcggacgatg
    21841 cgcctggtcg acgataccgg ccgcctgatc cgcgcccccg aggttcgttt ttcgtgcgac
    21901 gaaatcggtc tcgaactgtt cggctacaac atcgataacc gtacgctgct gacggcgctg
    21961 gaacaacgcg ccgcggagct ttcaaatctg acgcggttcg acgatgaggc ggagacgatc
    22021 agcccggatg acgctgacgt tgcaattcgt accggcgacg gaaaatcact gtccgcatgc
    22081 ctggtggtcg gcgccgatgg gcggcaatcg cactgccgcg aggccgtcgg catcgacgtc
    22141 cggcgccgcg aactcaatca ggccgcgttg accttcaata tcggccattc gcggccgcac
    22201 cgggatatct cgacggaatt tcatacacca caaggccctt gcgtgtttgt gccgctgccc
    22261 ggcgatcgct ccagcgtggt ctgggttgcg gctccgaaag aagccgagcg gctattcgcg
    22321 ttgaccgatg acgagttgtc ggaggccgcg gaacggcaat cgcattccat tctcggccgc
    22381 gtccacgtcg agccgggacg ccatttgttt ccgctggcga tcgaccagcc cagacaattc
    22441 gccaaatccc gcatcgcgct ggtcggcgag gccgcgcatg tgctgccgcc gatcggcgcg
    22501 cagggcctga acctgggatt gcgcgatgcc gccgatatcg ccgatatcgt gcgggaggcg
    22561 atgctgtcgg gcgaggatcc aggttcgccg caagtgctga agcgctacga taccgcgcgg
    22621 cgtgccgata ttgccagccg gacattcgcc atcgatgccg caaaccgctc gttgctcagc
    22681 gattttctgc cgatgcaatc gctgcgcgcg gccggcctgc atctgatcgg ttcattcgga
    22741 ccgcttcggc gcctcgccat gcgcgaggga ctggcgcctt cgtggcggcc gacgcggcgg
    22801 gctggctaaa gcgttttcga acgaaagcct gccccggacg cgatccgggg tggacgttcg
    22861 cttgaaagcg ctatctcacg gaaacaccag ccggcgggtc tggatcaacc acatcgcgct
    22921 ggtcagcgtg atgaccgagg cgaatgttcc gatcagcacg gcgaccgacg cgggcacgaa
    22981 ggcgctcatc gtgccgaggg tggttgcgac caggaatggc gggttgttga gtccctcgaa
    23041 gggcgtcttc gacattattc cgaacagcag tgcgggcagc gaaacataga gcaggaagaa
    23101 gttcatccac gccaatcccg tttccgggaa acccttggcc ctgccgcagg cgaagccgat
    23161 gaagatcagg ccgaaacacg gcaacgccag gttgagaacg tcgaccacgg gtgatacgtc
    23221 ttttcatgtt cattggcccc gatcggcggg gtggacggac cagcaatagc aagggttaat
    23281 tcgtccgttt gcctctagca tcggccacaa tcatggtcta ttcgacgagg tatgataaaa
    23341 gcccggaccg ccaaatttca gatcggacag atcgtccgtc accggatctt ttcgttccgg
    23401 ggcgtgatct tcgatatcga tccggtcttc aacaataccg aggaatggtg gttgtcgatc
    23461 cccgaggaga accggcctca caaggatcag ccgttctatc atctgctcgc cgagaattcg
    23521 gaatccgaat acgtcgccta cgtctccgag cagaatctgt tgcctgacga ttccggcgag
    23581 ccgatccggc actctcaagt cgccgagatt ttcgtcaagg acaaatcggg cggctatcgc
    23641 ccgcgcaacc catcgctgaa ttagtctttg caagattcgt tttggcaaga tcagtcttgg
    23701 caagcctagt cttggcaagg ctcgtgtccc ggacgcggtg cagcgtgctt cacgcctgct
    23761 ccgcacagcc gtgacccata agatcctcac aagcaagatg gaccccggat cagccgtgca
    23821 ccgcggagcc tgtgctcggc cgcgcgtcgc gcggacccga gtgcgctgcg cagcatccgt
    23881 ggcacgctgc aggatttccc cctgcaataa aaaaggcgct cgatcaaagc gccttttggg
    23941 tccgttgata aagagcagtt ccgcttgtta cttcgcagcc ggattggccg cagccgcgcc
    24001 gttgggcggg ttagcctcga gccgcttgcg cgcttcctcg gcgcgtttct gcaactcttc
    24061 ctgcagcttc ttctgggtct cctcgaacac cttcgggtcg gtcggcgggc catcataggc
    24121 cttggcgaat tccgcgagcg gcagcggcag ggtgagcggc gcgccgttgg agttgatcgc
    24181 ctgaaccacc aaattctgcc ccttcttcat gttcgcgagc agttccggcg tgacttcgta
    24241 gtccgacatg cagccgttgg cgaagcagat cacatagggg ctctgcgccg gcggattgcc
    24301 gtcgacaatc acgcgggttc cgtgaaccag ctgcataccc agcggaagcg tgacgcgcag
    24361 gatcttcttc ggttcgcctt ccggttcgat gatcacagct gcaatcaccg gttgaccgga
    24421 ctcgatgcga ccgtccttgc cggtgaagca gacctgcttg gcatttgcgt cctggccctt
    24481 caggcagaac ttggtccaag gcgcatagat cagctgaacc tgctggtccg ggggctgggc
    24541 accggccgcg ccagccgccg gcggggcctg ctgctgctga gcggtcggtg cgggcgccgc
    24601 aggagccgcc tttggcgcgg ctttcggcgc agccttggcc ttcggtgcag caggagcgcc
    24661 gggtgcaggc gcctgggctt gcacgtctgg agccagggat gcggcggcca gtgccgttgc
    24721 cgccaacaga gcgaaaaccc gcccacgcgg ccataccggc gcggccaaga tacggaaatt
    24781 cattgcggaa aaccctttct gaacggaagc gcccgaaacc gctacaggca ccgagacctc
    24841 gccgtttggg tccggctgtc tccttgccaa ttgaggcgga tacgtgacag gcccggccgt
    24901 cttggccaat ggcacgtctt catacagtgg caggcgaaaa gatcaatggc ggcgggcatc
    24961 tttgccgcgc gccgggaccg gatatgacgg ctatgataag acttgcgtgt ggccaatatc
    25021 gaatcgaatc agcacgcgtc attgcgagtt tccaggtggc ggcgccttcg agccgtggcc
    25081 ggtgcgatca tcggtatctg gctcgcgccg gatccctgcc atgccggcga caactacgcc
    25141 atcgccatgc atggcgcgcc ggccttgccg gccgatttca cccacatgcc ctatgccaat
    25201 cccgatgcgc cgaagggcgg ccggctggtg cagggcatcc tcggtacctt cgacagcctc
    25261 aaccccctga tcgtcagggg cgtagccgtg cagcaggttg ttgccgtgca gcaaatgaga
    25321 ggcgtcgtga tcgagagcct gatggcgcgg agcaatgacg aggccttcac gctctacggt
    25381 ctcctggcca ggagcgtgga gaccgacgac atcaggagtt acgtgacctt ccatatcgat
    25441 ccgcgggcgc gcttctcgga cggccaaccg gtaacggccg aggatgtgct gttttcgtgg
    25501 gcgttgctgc gcgacaaggg ccgtccacac catcgccagt attattccaa ggtagcgaag
    25561 gccgaggcac ccgacccgct cacggtgcgg ttcgacctcg gtgctgccaa tgaccgcgaa
    25621 ttgccgctga tcctcggact gatgccgata ctgcccaagc acgccgtcga tgtcgcgacg
    25681 ttcgaagaga ccacgatgac gcccttgatc ggttccgggc cctaccgcgt cagcgcggtc
    25741 aggcccggcg ccagcgtgac gctgacgcgc aatcccgatt attggggcct taacctgccg
    25801 gtcaatcgag gactatggaa tttcgacgag atcaggctcg atttctatcg ggaggccaac
    25861 ggcgagttcg aagcgttcaa gcgcggcctc tatgactttc gcgtcgagaa cgaaccgctg
    25921 cgctggcacg acggatacga ctttccggcg gcccgcaatg gcgaggtgat ccgcgacacc
    25981 ataaagaccg ggatgccgca accttcggaa ttcctggtgt tcaacacgcg gcgcccgatg
    26041 ttttccgata tccgcgtccg ccaggccttg acgctgctgt tcgatttcga atggatcaac
    26101 cgcaattatt tcttcgggct ctatacgcgc tcggcgggct tctttgcggg gtccgaatta
    26161 tcggcgtatg gccgcgccgc tgacgaccgc gagcgggagt tgttgaaacc atttgtttcg
    26221 cacataccac cggatattct caacggcagc taccgccttc cggtcagcga cggctccggc
    26281 cgcgatcgcg taacgctccg cagcgctctg aacctgttgt cggaggccgg ctacgatctc
    26341 gacgggacgg tgttacggca gcgttcgacc aaagcccccc tcaccttcga gattctggtg
    26401 acgacccgcg atcaggagcg gatcgcgctg gcctatacgc gcgatctgaa gcgcgcaggc
    26461 atcgaggcca aggtgcgcgc ggtcgatgcc gtgcaattcg aacaacgccg gctcggcttc
    26521 gatttcgaca tgatccacaa cagatgggat caatcattgt cgcccggcaa cgaacagtgg
    26581 ttctattggg gaagcgaggc tgccgaccat cagggcacgc gcaattatat gggcgcaaaa
    26641 gaccccgcca tcgacgccct gatcgcggca ctgctggagg cgcgcgagca tccggctttc
    26701 gtctcggcgg tgcgggcgct cgaccgggcg ctgatgtcgg gcttctacgc aattcccctg
    26761 tttaacgtcg gggaacaatg gattgcgcgc tggaatcgga tagaacggcc tgtgaccacc
    26821 gccctgacgg gatatctacc ggaaacctgg tggcaaaacg ccggcccctc aagcaaagtg
    26881 atcccgtgat tcagaccaac gcctcgccaa cgctcgacgg actgtttcga cggatcctgg
    26941 ctcgcaagcc ggatgcgctg gcgctgctcg atccgctcaa caagcaacgc atcaccggcc
    27001 agccgccaaa acgcctgacc ttcgcgcagg ccgatcgcgc catctccgcg ctggcggcgc
    27061 atttcatcga agcgggcttg ccgaccaatt cggtggtcgc ggttcaactg ccgaacaccg
    27121 tcgaattcat gctgacggtg ctcgccgcgc accgcgccgg gctgatcgtc gccctgcttc
    27181 cgctgctttg gcggcaagcc gaactgacgg tcgcgctcaa tcgaaccggt gcccgggcga
    27241 tcgtgaccat gggcagggtc gacggcgtgg ttcatgccga cctcgccatg aacgccgcgg
    27301 ccgaatcatt ctcgatccgc cacgtcggtg gcttcggcaa cgaccttccc gaaggcatgg
    27361 tgtcgctcga tcttgccctc gcaggtcaat cggaaaccac ccgcgccatc acccaggatg
    27421 gccgcagggc gtcgatcatt tcgttcgacg tgacggtcga tggttttcgc gcggtaccgc
    27481 gcacgcatc
//
LOCUS       DATNOI010000013         3105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_885726,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000013 DATNOI010000000
VERSION     DATNOI010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885726"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..559)
                     /locus_tag="VN968_00490"
     CDS             complement(38..559)
                     /locus_tag="VN968_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HXL27760.1"
                     /translation="MTAGNDFDPQAPQPELFSALLTPHRSLNRTGFLVLMGFLSTVSF
                     AAGLAFLLMGAWPVFGFFGLDVLVIYWAFRVNFRDAKATEEIRVTPSELRIRRVSHRG
                     QVVEWVLNPLWVQLDQKTHAEFGIEKLYLVSKGRSISIAQFLGADEKASFAKALNAAL
                     QAARRGPTYNPVR"
     gene            582..1400
                     /gene="nth"
                     /locus_tag="VN968_00495"
     CDS             582..1400
                     /gene="nth"
                     /locus_tag="VN968_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXL27761.1"
                     /translation="MAKITRRPRPKSKLRDPKPAQKSCSRPAAKSGRAPAAKKSLKVL
                     TKARASKSRASKSPAPKPWTPAEVHEAFSRFRKANPEPKGELEHLNPYTLLVAVVLSA
                     QATDAGVNKATRALFAVADTPQKMLALGEEKLRDCIRTIGLYRNKAKNVIALSEKLIA
                     EFGGEVPRTRAEIESLPGAGRKTANVVLNMAFGEHTMAVDTHVFRVGNRTGLAPGDTP
                     LEVELGLERVIPPEFMLHAHHWLILHGRYTCLARQPRCEVCLINDLCRWPEKTI"
     gene            1498..2673
                     /locus_tag="VN968_00500"
     CDS             1498..2673
                     /locus_tag="VN968_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HXL27762.1"
                     /translation="MSQQDQISPAPWAREPGSTPSPPAAPAAASPSRPSGSHPSWGRL
                     AIPLFAVLVAFGFIALAMLRWDAWVGSEVIQSTNDAYVRAELTRLSSRVAGEVLTVAA
                     NDFQRVKAGDLLVQIDPADYQAQVAQAEAGVIGAQAALDNLANQVELQYATIAQGEAQ
                     QLSALAQEVEARQEQERQQSLTQTEAGTRQKLEQATAGYARAQADVRASRALVAAQRH
                     QLEVLSGTKKQRAADLQGAKAALAAARLKLGYTKIVAPFDGVVGERQVQPNDYVNIGS
                     NLINVVPLPDVYVMANYKETQLTRVKPGQPVEVTVDSFPSEKLHGRVERIAPASGSQF
                     ALLPPDNATGNFTKVVQRIPVRIEFDKGQPLLERLLPGMSVVTRIRTDEAGTDGGGK"
     gene            2657..>3105
                     /locus_tag="VN968_00505"
     CDS             2657..>3105
                     /locus_tag="VN968_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL27763.1"
                     /translation="MAAESSDAGHAGQGPLSRGGIAPQPLFAVGAVLLGSFLANFDSR
                     LTSVGLPDLRGAFSLSFDEGAWLSTAAIGSQIFIAPAVAWLATVFGLRRILGIPSLVY
                     ALVSLVIPFVRDYNALIVLSIVHGLLLGTFVPATLMIVFRNLPIRWWL"
BASE COUNT          580 a         1026 c          979 g          520 t
ORIGIN      
        1 ggcgcggtgt ccaaaccacc cgattcccga cagtatgtca gcggacggga ttataggtcg
       61 ggccgcgtcg cgcggcctgc agggcggcat tcaaggcctt ggcaaagctc gccttttcgt
      121 cggcgcccaa aaattgcgcg atcgagatgc tgcggccttt cgacaccaga tagagctttt
      181 cgatgccgaa ttcggcgtgg gttttctgat cgagctggac ccacaaggga ttgagcaccc
      241 attcgaccac ttggccgcga tggctcaccc ggcggatgcg cagttcggat ggcgtcaccc
      301 ggatttcctc ggtggccttg gcgtcgcgga aattgacccg gaacgcccag tagatcacca
      361 gcacatcgag gccgaaaaag ccgaacaccg gccatgcgcc catcagcaga aacgccagcc
      421 cggcggcaaa gctgaccgtg ctgagaaacc ccatcagcac cagaaaaccg gtgcggttca
      481 acgagcggtg cggcgtcagc agcgccgaaa acaattcggg ttgcggcgcc tgcggatcaa
      541 agtcgttgcc tgcggtcatc gcgtatcagt atatcccgat catggcgaaa atcacccgca
      601 gaccccggcc caaatcgaaa ctcagggatc ccaaacccgc ccaaaaatct tgcagtcgtc
      661 ccgcggccaa atcggggcgc gcgccggcgg cgaaaaaatc gctcaaggtt ttgaccaaag
      721 ccagggcttc caaatccagg gcttccaaat ctccggcgcc caaaccctgg acgccagccg
      781 aagtccacga agcgttcagc cggtttcgca aagccaatcc cgagccgaag ggagagctgg
      841 aacatctcaa cccctatacc ctgctggtgg ctgtcgtgct gtcggcgcag gcgaccgacg
      901 ccggcgtcaa caaagcgacg cgtgcgctgt ttgccgttgc cgacacgccg caaaagatgc
      961 tcgcgctcgg cgaggaaaaa ctccgcgact gcatcaggac catcgggctc taccgcaaca
     1021 aggcgaagaa cgtcatcgcg ctgtcggaaa aactgatcgc cgagttcggc ggcgaggtgc
     1081 cgcgtacccg cgctgaaatc gaatcgctgc ccggcgccgg ccgcaagacc gccaacgtcg
     1141 tgctcaacat ggctttcggc gaacatacca tggcggtgga cacccacgtg tttcgggtcg
     1201 gcaatcgcac cgggctggcg ccgggcgata caccgttgga ggtggagctt ggtcttgagc
     1261 gggtgattcc ccctgagttc atgctgcacg cgcatcactg gctgatcctg cacggccgct
     1321 acacgtgcct ggcgcgccag ccccgctgcg aggtatgcct gatcaacgat ctctgccgct
     1381 ggccggaaaa gacgatttga ggcatcatca ccgtcatgtc tggccgcacg gtccggcatg
     1441 cgggatcttc cctcggttca tcgcaagggg gagaatgatt ttgaagaggg ggtccctttg
     1501 agccagcagg atcagatcag tccggcgcca tgggcgcgtg agcctggatc aacgccctcc
     1561 ccgccggccg ctcccgcggc agcttcacca tcgcggccgt ccggttcgca tccttcgtgg
     1621 ggacgcctcg cgattccgct attcgccgtg ctggtcgcgt tcgggttcat cgcgctggcg
     1681 atgctgcgct gggatgcctg ggtcggcagc gaagtgatcc agagcacgaa cgacgcgtat
     1741 gtccgggccg aactgacacg gttgagcagc cgggtcgccg gtgaagttct caccgtcgcc
     1801 gcgaacgact ttcagcgcgt caaggccggc gatctcctgg ttcagatcga tcccgctgac
     1861 taccaggccc aggtcgcgca ggccgaggcc ggcgtgatcg gcgcgcaggc ggcgctcgac
     1921 aatctcgcca accaggtcga gctgcagtac gccaccattg cgcaaggcga ggcgcagcag
     1981 ttgtccgcgc tcgcccagga ggtcgaagcc cgtcaggagc aggagcggca acagtcactg
     2041 acccagaccg aagccggcac aaggcaaaag cttgagcagg ccaccgcggg ctacgcccgc
     2101 gcccaggccg atgttcgggc gagccgggcc ttggttgcgg cgcagcgcca ccaactcgag
     2161 gtgctgtccg gaaccaagaa gcagcgcgcg gcggacctgc aaggcgccaa ggctgcgctg
     2221 gccgccgccc gcctcaagct gggctacacc aaaatcgtcg caccgttcga cggcgtcgtc
     2281 ggagagcggc aggttcagcc gaacgactac gtcaatatcg ggagcaacct catcaacgtc
     2341 gtgccgctcc ccgacgtcta cgtgatggcc aactacaagg agacccaact gacgcgggtg
     2401 aagccgggcc agcctgtcga ggtgaccgtc gacagtttcc caagcgagaa actgcacggc
     2461 cgcgtcgagc gaattgcgcc cgccagcggc tcgcaattcg cactgctgcc gcccgacaac
     2521 gccacgggca atttcaccaa ggtggtccag cgcatcccgg tccggatcga gttcgacaaa
     2581 ggccagccct tgctggagcg cctgctgccg ggaatgtcgg tggtcacacg aattcgcacc
     2641 gacgaagcgg ggacggatgg cggcggaaag tagcgacgcc ggccacgccg ggcaggggcc
     2701 gctgtcgcgc ggcggcatcg cgcctcaacc gctgttcgcg gtcggcgcgg tgctgctcgg
     2761 gtcgtttctc gccaatttcg acagccggct gacctcggtc ggattgccgg acctgcgcgg
     2821 cgccttttcg ctcagcttcg acgagggcgc ctggctctcg acggcggcga tcggctcgca
     2881 gatcttcatc gcgccggcgg tggcctggct cgcgacggtg ttcggcctgc ggcgtattct
     2941 cggcattcca agcctggtct atgccctggt ctcgctggtg atcccgttcg tgcgcgacta
     3001 caacgcgctg atcgtgctca gcatcgttca cggcctgttg cttggcacct tcgtgcccgc
     3061 caccctgatg atcgtctttc gcaacctgcc gatccgatgg tggct
//
LOCUS       DATNOI010000014        10520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1069471,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000014 DATNOI010000000
VERSION     DATNOI010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10520
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069471"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="VN968_00510"
     CDS             complement(<1..1214)
                     /locus_tag="VN968_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXL27764.1"
                     /translation="MTREVQRDPQSAISRRTLVHGLAIAAGATVVGTSGALAQQGGEL
                     GPPTTITTPPRDFGPGGAPTTYFWDPDIIAVDPSFNDLAQPNTAIKRLYTGLLWAEGP
                     AWSAQGRYLLWSDIPNNRQMRWSEDDGRVSVFRSPSNNSNGNSFDFQGRQLSCEHLTR
                     RVVRYEHDGTSSVLADNFGGKKLNSPNDVVAHPDGSYWFTDPPYGGQLYEGEPDAPGG
                     ASNPAGKLNPRIGQPAGFVPGKRELPTNCYRVDPSGRIDLVVSEDQVPDPNGLAFSPD
                     YKKLYVISTGKGPGDTGPGGKGDMFVFDVGADNKLTNQKRFSDFTIDGVKCGPDGVRC
                     DVSGNVWASSNAGRHVGYSGVTVWTPDGKLIGRIRLPEVCGNICFGGPKRNRLFMAAS
                     QSLYAVYTATQGA"
     gene            complement(1510..2712)
                     /locus_tag="VN968_00515"
     CDS             complement(1510..2712)
                     /locus_tag="VN968_00515"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL27765.1"
                     /translation="MTRDNNPNPNRYDVIILGAGAAGLMCAAVAGGRGHQVLLLEQAR
                     NPGEKIRISGGGRCNFTNLHTAPANFLSANPRFCHSALSGYTQRDFMALVESHGIAYH
                     EKTRGQLICDGPSQQIIDMLLEECRKVQARLRLGVRVSAISRSENGFVVGSDQGEFRA
                     RSLVVATGGPSIPKMGSSGFGYKIAEQFGLKIVPPRPALVPLTFDAALLAQFKDLSGV
                     SVDAVASCGKIRFDEALLFTHRGLSGPAILQVSSYWREADDIVIDMAPGSDVLAGLKR
                     LRRDHPRQELATALSNFLPKRLARTIAEGIGGPERIADYSDLHLANVAAGVKQWRVRP
                     NGTEGYRTAEVTLGGVDTSELSSKTFESRSVPGLYFIGEVVDVTGHLGGFNFQWAWSS
                     GYAAGRHV"
     gene            complement(2709..3587)
                     /locus_tag="VN968_00520"
     CDS             complement(2709..3587)
                     /locus_tag="VN968_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HXL27766.1"
                     /translation="MIYDIRHVTTYSYESPVSFARCSLRLEPRSGDGQQLVSHGVDIR
                     PRPVDRTIRHDFFGTHTESVLIETAHRHLRIDSRSRVSVSRRAPARTAPSPAWESVRD
                     VAFESTSLGPESPIGYAFASPLVPVQQAVTLYAAMSFPPASGILAGAVDLMHRIRTEF
                     KYDPKATVISTPLDEVFEKRHGVCQDFAHVMIAGLRGLGLPAAYVSGYLRTIPPPGKP
                     RLQGADATHAWVSLWCGVEIGWIGFDPTNDILVENDHIVLAVGRDFSDVSPVDGIIVG
                     SRRQKLTVAVDVLLVE"
     gene            complement(3584..6109)
                     /locus_tag="VN968_00525"
     CDS             complement(3584..6109)
                     /locus_tag="VN968_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HXL27767.1"
                     /translation="MAGHAGQGSGQGNSQGSKARPGNRRVAQWTRGYARLPGIPDEYI
                     AQDGTPRAVWTRFFDAFAELTPGDIERRFGSADRHLREAGVTYRAPGETADRIWPLSH
                     LPLLIGEADWQQLTAGIVQRAQLLELVLGDLYGEGRLVAENAVPAAAIAGSTEYLRSV
                     CGIKPPGGRYLNLYAADVGRGPDGRWWVLGDRTQAPSGAGYALENRLVLSRAFTTLYR
                     SMNVERVAPFFEAFRDALRASSDRDEPRIGLLTPGIFSETYFEHATLARYLGFLLVEG
                     DDLAVSGDRIHIRTVAGLKRLDVLLRRVDSNSLDPLELDASSQLGVPGLIDVLRKNGV
                     VVANMPGSGVMEARALLGFLPSLSQRFFGEDLKMPHIATWWCGQKSAREEVLSRLDHV
                     AIEGAYGGGVPCFGRGPVLACELSASERERLKTAIHDRGIDYVGQELVRLSTTPVWDN
                     GRITPRPFVLRVFAAATPGGWTMMPGGFCRIADQLDARAVSMGEGARAADVWVVSDKA
                     ISMATLLPSGDNVRIRRIAGWVPSRAADNLFWLGRYLERAEATLRLVRALGTQRDPGK
                     GAATVPHSVERIQRLLVTWGAVSQATRAQPAKVAAEALRSEDKFGSALSLVRAAQRTA
                     TSLRERLSPDAWQIITEMAERLVQEVDDDDAVIVAAELTLQELASFAGLAQENMNRAA
                     GWRFLEMGRRAERAINTVRFARQFAYDEAAGDDLDILLTLVDCQITYRSRYLLGPLLA
                     PVRDLVVLDPYNPRSVAFQVFALNDHIASLPTLKEGGLIERPHRLAVALQATLTTAEA
                     AVLDTKSLFALEQDLLNLADAIGSHYFPHGPNASRPEKLTGLA"
     gene            complement(6139..9426)
                     /locus_tag="VN968_00530"
     CDS             complement(6139..9426)
                     /locus_tag="VN968_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HXL27768.1"
                     /translation="MSIFVALHHVTHYKYDRPIDLGPQTVRLRPAPHTRTPILSYSLK
                     VTPSNHFVNWQQDPQGNWLARFVFPEKATELKIEVDFTASMTVINPFDFFVEPSADSF
                     PFEYTNDLKTELAPYLATIEPGPLLAKYLADIPREAASTVNFLVDLNAQLQKKIRYII
                     RMEPGIQTPEQTLELGAGSCRDSAWLLIQIFRHLGLAARFVSGYLIQLRPDIDPVEGP
                     REVENDFTDLHAWAEVYLPGAGWIGFDVTSGMLTGEGHIPVAATPHYRSAAPISGAVG
                     FAEVEFGFEMSVKRIREAPRITKPFSDESWARLDQLGEQVDADLKAGDVRLTMGGEPT
                     FVSVDDLESPEWNIAAVGPTKRGLADDLIRKLRARFAPGGLLHFGQGKWYPGESLPRW
                     AFGLYWRKDGVPIWKSSDLIARIEGPRKAGIADAERFAEGAAKKLGVDSEFIMPAFED
                     PSHWLQKEAALPPNVDPSDSKLSDPEERSRMARVFDQGLNVPKGFVLPIQRWNADAGK
                     SSSRWKSERWKLRRGNLFLTPGDSPLGLRLPIASLPHIPAEEFPYVVEQDPMEPRDAL
                     PVYDATALVEPAPHQEVQEQQLKGGGIRTAMSIEIRDGILCAFMPPVEKLEDYLELVT
                     AVEATAEEMQMQVHVEGYPPPFDPRVEVIKVTPDPGVIEINIQPAQNWREAVDITFGL
                     YEDAAQVRLGANRFLVDGRHTGTGGGNHVVVGGSTPADSPFLRRPDLLKSLVLYWQRH
                     PSLSYLFSGMFIGPTSQAPRIDEARHDGLYELEIALAHVPPPGIKAPLWLVDRLFRHI
                     LVDITGNTHRAEICIDKLYSPDGTTGRLGLVEFRALEMPPDPRMSLAQQLLIRALIAK
                     LWREPQQGNFVRWGTTLHDRFMLPHFLWEDFLGVLAELRQSGYDVSPDWYAAQLEFRF
                     PAFGRVHHGGVSLEVRQALEPWHVLGEEASAGGTVRYVDSSVERLQVKASGFVEGRHV
                     VTCNGRRMPMTQTDRSGEAVAGVRFKAWQPASGLHPTIPVHAPLTFDLIDTWNGRSLG
                     GCVYHVAHPGGRSYDTKPVNSYEAEARRLARFQDHGHTPGKIDPPREERTLEFPLTLD
                     LRTQLLH"
     gene            complement(9594..>10520)
                     /locus_tag="VN968_00535"
     CDS             complement(9594..>10520)
                     /locus_tag="VN968_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HXL27769.1"
                     /translation="VVLGLGCEVNQIGGLMKEQKLAGRLRELEIQEIGGTRKTVEAGV
                     AFVREALTDANNVKREPVSASELTVALQCGGSDGYSGVSANPALGAASDLLVRHGGTV
                     ILSETPETYGAEHLLTRRAVSREVGEKLVALMRWWEEYTAREGAEMNANPSPGNKAGG
                     LTTILEKSLGAMAKAGSTNLVDVVNYAEQITKKGFVFMDTPGYDPVAATGQVAGGANL
                     VCFTTGRGSVFGCKPAPSIKLATNSAMFKRMEDDMDVNCGTILDGEEMVQACGQRIFD
                     LILKTASGQPTKSESFDFGGAEFAPWVLGATM"
BASE COUNT         1919 a         3390 c         3369 g         1842 t
ORIGIN      
        1 gcgccttgcg tggcggtata cacggcgtag agcgactggc tcgcggccat gaacaggcgg
       61 ttgcgcttgg ggccgccgaa gcagatgttg ccgcacacct cgggcaggcg gatccggccg
      121 atgagctttc cgtccggcgt ccataccgtc acgccactat agccgacgtg gcggccggca
      181 ttgctcgagg cccagacatt gccgctgaca tcgcagcgca cgccgtccgg tccgcacttc
      241 acgccgtcaa tcgtgaaatc gctgaaccgc ttctggttgg tgagcttgtt gtcggcgccg
      301 acatcgaaca cgaacatgtc gcccttgcca cccggaccag tatcgcccgg ccctttgccg
      361 gtactgatga cgtacagctt cttgtaatcg ggcgagaacg ccagcccgtt gggatccggc
      421 acctggtcct cgctcaccac gagatcgatg cggccgctgg gatcgacgcg atagcagttg
      481 gtcggcagct cgcgtttgcc cggcacgaag ccggccggct ggccgatccg tggattgagc
      541 ttgccggcgg gattgctggc gccgcccgga gcgtcgggct cgccctcgta gagctgaccg
      601 ccatagggcg ggtcggtaaa ccagtagctg ccatccgggt gggcgacgac gtcgttgggc
      661 gagttgagct tcttgccgcc aaaattgtcg gcgagcacgg acgacgtgcc gtcgtgctcg
      721 tagcgcacga cccggcgggt gagatgctcg caggagagct ggcggccctg aaagtcgaac
      781 gagttgccgt tggagttgtt ggacggcgag cggaacacgc tgacgcgacc gtcgtcctcc
      841 gaccatctca tttgccgatt gttgggaatg tcgctccaca gcagatagcg gccctgcgcg
      901 ctccacgccg ggccttcagc ccacagcaat ccggtataga gacgcttgat cgccgtgttg
      961 ggttgggcga ggtcattgaa cgacggatcg accgcgatga tgtcggggtc ccagaaatag
     1021 gtggtcgggg cgccgccggg gccgaaatcg cgcggcggcg tggtgatcgt cgtcggcggc
     1081 cccagttcgc cgccctgctg ggccagcgcg ccgctggtcc cgaccacggt ggcaccggct
     1141 gcgatcgcca ggccgtggac gagcgttcgt cgtgaaatcg ctgattgcgg atcgcgctgc
     1201 acttcgcgtg tcattacatc ctcccaatat atgcgccggc cggttgatcc ggccgagcgg
     1261 ccgtaacgct aacccggtcc gggtccggtt gcgagaaccg ctttcgattc agctgacggc
     1321 tgaagcgggc aaagtgcgaa ccagcgaaat gtccgaagct gggtaacccg gactcgtcgg
     1381 ccgccagcgc atgatccgga aaagcatgcc ttcgggcttg acccgagggc ggaaaccggt
     1441 tttccgaaaa gatcatgctt aaacaaagag atgagaccat gatccgattc aatctaatcg
     1501 gatcatggtc tagacgtgcc ggcccgccgc gtatcccgag gaccaggccc attgaaaatt
     1561 gaagccgccg aggtgaccgg tgacgtcgac gacctcgccg atgaaataaa gcccggggac
     1621 ggacctggat tcgaaggttt tcgacgagag ttccgatgta tcgacaccgc ccaacgtcac
     1681 ctccgcggtg cggtagccct cggtcccgtt gggcctgacg cgccactgct tcactccagc
     1741 ggcgacgttc gcaagatgca gatcggaata gtcggcgatc cgttcaggac ctccgatgcc
     1801 ctccgcaatg gttcgcgcca gccgtttcgg cagaaagttg gaaagcgcgg tcgccagttc
     1861 ctgcctcggg tgatcgcgac gcagccgttt cagcccggcc aggacatcgc ttcccggcgc
     1921 catgtcgatg acgatgtcat cggcttcacg ccaataggag gaaacctgca ggatggccgg
     1981 accgctcagc ccgcgatgcg taaacagcaa cgcttcatcg aagcggatct tgccgcaact
     2041 cgcgaccgca tcgaccgaga cgcccgacag atccttgaac tgcgccagca atgccgcatc
     2101 gaacgtcaaa ggcaccagcg ccggccgcgg cggcacgatc ttcaggccga attgctcggc
     2161 aattttgtac ccgaacccgc tcgaccccat ctttggaatg gaaggaccgc cggttgccac
     2221 caccagcgat cgtgcgcgaa actcgccttg atcggaccca acgacaaaac cgttttcgct
     2281 gcgggagatc gcggagacgc gcacgccgag acgcaaccgc gcctggacct tacggcactc
     2341 ctcaagcagc atgtcgatga tttgctgcga gggcccgtcg caaatcaatt gcccgcgtgt
     2401 cttttcatga taggcgatgc catggctctc gaccagcgcc atgaaatcgc gctgggtgta
     2461 gccgctcagc gcggaatggc aaaaccgcgg attggccgag agaaaattcg caggcgccgt
     2521 gtgcaggttg gtaaaattgc agcggccgcc gccggaaatc cttatcttct cgcccgggtt
     2581 tctggcctgc tccagcagca gcacctgatg tccgcgcccg ccggcgacag ccgcgcacat
     2641 caggcccgcg gcccctgccc cgaggatgat gacgtcgtaa cggttcgggt tcggattgtt
     2701 atcccgcgtc attccaccag cagcacatcc actgccaccg tgagcttttg cctgcgcgat
     2761 cccacaatga tgccgtcgac cggtgaaacg tcggaaaaat cccgccccac cgccagcacg
     2821 atgtgatcgt tctcgacgag aatgtcgttg gtcggatcaa agccgatcca gccgatctcc
     2881 acgccgcacc acagcgagac ccaggcgtgg gtcgcgtccg cgccttgcag gcgcggtttg
     2941 ccgggcggcg gaatggtgcg cagatagccg ctgacatagg cggccggcag tccgagaccg
     3001 cgcagccctg cgatcatcac atgggcgaaa tcctggcaga cgccgtggcg tttctcaaac
     3061 acctcgtcaa gcggcgtcga gatgacggtt gctttcgggt cgtacttgaa ctcggtgcga
     3121 atgcgatgca tcaggtcgac cgcgccggcc aggattccgc ttgcgggcgg aaagctcatg
     3181 gccgcataca gggtgaccgc ctgctgcacc ggcaccagcg gactggcaaa ggcataaccg
     3241 atcggcgact ccggaccgag actggtggac tcgaatgcca cgtcgcggac gctctcccat
     3301 gccggacttg gcgcggtgcg ggccggggcg cggcgggaca ccgagacccg cgaccttgaa
     3361 tcgatgcgaa ggtggcgatg cgccgtttcg atcaggacgc tttcggtatg cgtgccgaaa
     3421 aagtcgtgcc gtatggtgcg gtcgacgggc cgtggcctga tgtcgacgcc gtgcgagacc
     3481 agttgttgtc cgtcgccgct cctcggctcc agccgcagcg agcagcgcgc gaagctgacc
     3541 gggctttcgt agctgtaggt ggtaacgtgg cggatgtcgt agatcacgcg aggcccgtga
     3601 gcttttcagg ccggctcgca ttgggcccgt gcggaaaata gtgcgatccg atggcatcgg
     3661 cgaggttgag cagatcctgc tccagcgcga acagcgattt ggtgtcgagc acggcggcct
     3721 ccgccgtcgt cagcgtcgcc tgcagggcga cggccagacg atgcggccgc tcgatcagtc
     3781 cgccctcttt caatgtcggc aggctggcta tgtgatcgtt cagcgcaaaa acctggaacg
     3841 cgaccgacct tggattgtaa gggtccaaga cgacaaggtc gcgcaccggc gccagcagcg
     3901 gcccgaggag gtagcgcgag cgataggtga tctggcagtc caccagcgtc agcaggatgt
     3961 cgagatcgtc gccagcggct tcgtcatagg cgaactggcg cgcgaaccgc acggtgttga
     4021 tggcgcgctc ggcgcggcgg cccatttcga gaaagcgcca gccggcggcg cggttcatgt
     4081 tttcctgtgc cagacccgca aagctcgcca gttcctgcag tgtcaattcg gcggcgacga
     4141 tgacagcatc atcatcgtcg acttcctgga cgaggcgttc ggccatctcg gtgatgatct
     4201 gccaggcatc cggcgaaaga cgctcgcgta acgatgtcgc ggttcgctgt gccgcgcgca
     4261 ccagcgacag cgccgagccg aatttgtcct cgctccgcaa cgcctcggcg gcgaccttcg
     4321 ccggctgcgc ccgcgtcgcc tgcgacacag caccccaggt cacaagcaac cgctggattc
     4381 gctcaaccga gtgaggaacg gtggctgccc ccttgccggg atcgcgctgc gtcccgagcg
     4441 cccgcaccag ccgcagcgtc gcctcggcgc gctcgagata gcggccgagc cagaacagat
     4501 tgtcggcggc acggcttggc acccagcccg cgatgcgccg gatgcggaca ttgtcccctg
     4561 atggcagcaa ggtcgccatc gaaatcgcct tgtcggaaac cacccagacg tctgcggcgc
     4621 gcgcgccttc gcccatcgac accgcgcggg catcgagttg atcggcgatc cggcaaaagc
     4681 cgccaggcat catggtccag ccaccgggag tagcggccgc aaaaaccctc aagacgaacg
     4741 gccgcggcgt gatgcggcca ttgtcccaca ccggggtcgt cgacagccga accagttcct
     4801 ggccgacata gtcgatgccg cgatcgtgga tcgcggtctt cagccgttcg cgctcgctcg
     4861 ccgacaactc gcaggcgagc acgggtcctc tgccgaaaca cggaacgccc ccgccatagg
     4921 cgccctcgat ggccacatgg tcgagccgcg acagcacttc ctcgcgggcg gatttctggc
     4981 cgcaccacca agtcgcgatg tgcggcatct tcaaatcctc gccaaagaaa cgctggctga
     5041 ggctgggcag gaagccgagc agcgcccgcg cctccatcac acccgagccc ggcatgttgg
     5101 cgacgacgac gccgttcttg cggagcacgt cgatcaggcc gggcacgccg agctgcgagg
     5161 aagcgtcgag ctcgagcgga tcgagcgaat tggaatcgac ccggcgcagc agcacatcga
     5221 gccgcttcaa gccggcaacg gtacggatat gaatgcggtc gccgctgacc gcgagatcgt
     5281 cgccctcgac cagcagaaat ccgagatagc gcgccagcgt ggcgtgttcg aaataggttt
     5341 cactgaatat gccgggtgtc agcagcccga tgcgaggttc gtcgcggtcg gagctggcgc
     5401 gcaaggcatc gcggaacgcc tcgaagaatg gcgccacccg ttcgacgttc atcgatctgt
     5461 agagcgtggt gaaagcccgc gacaacacca gccggttttc cagcgcgtag cctgcgcccg
     5521 acggcgcctg ggtgcggtcg ccgagcaccc accagcgccc atcgggtccg cgaccgacat
     5581 ccgcggcata cagattgaga tatcgcccgc cgggcggctt gatgccgcaa accgagcgca
     5641 gatattcggt gctgccggca atggcggccg ccggcaccgc gttttcggcc accaaccggc
     5701 cctcgccata gagatcgccg agcaccagtt ccagcaactg ggcgcgttgc acgatgccgg
     5761 cggtcaattg ctgccaatcg gcctcgccga tcagcagcgg cagatggctc aacggccaaa
     5821 tccggtcggc ggtttcgccg ggtgcgcgat aggtcacccc ggcttcccgc agatgccggt
     5881 cggccgatcc gaaacgccgc tcgatgtcgc caggcgtcag ctcggcgaag gcatcgaaga
     5941 agcgggtcca gaccgcgcgc ggcgtcccgt cctgcgcaat gtactcatcg ggaatacccg
     6001 gcagccgggc atagccgcgc gtccactgcg ccacgcggcg attgcccgga cgggctttgc
     6061 tgccttggct gtttccttga ccgcttccct ggccggcgtg tcccgccatc ccgactctcc
     6121 tgccctgagg gctcattctc aatgcaggag ctgggtcctc aagtcgaggg tcaagggaaa
     6181 ttcaagggtg cgttcctcgc gcggcggatc gatcttgccg ggggtgtggc catgatcctg
     6241 aaaccgggcc agccgccgcg cctcggcttc ataggaattg accggtttgg tgtcataact
     6301 gcgcccgccg gggtgcgcga catgatagac gcagccgccc agcgaccggc cgttccaggt
     6361 atcgatcagg tcgaatgtca gcggcgcatg aaccggaatg gtcggatgca accccgacgc
     6421 cggctgccac gccttgaagc ggacgcccgc gaccgcctcg cccgaacggt cggtttgtgt
     6481 catcggcatc cgccggccgt tacaggtgac gacgtggcgg ccttcgacaa aaccagaggc
     6541 cttgacctgc agccgctcca ccgacgaatc gacatagcgc acggtgcctc cggccgaggc
     6601 ctcttcgccg agaacatgcc agggctcgag cgcctggcgc acttccagcg agacgccgcc
     6661 atgatggacc cggccgaacg ccggaaagcg gaattcgagc tgtgccgcat accaatccgg
     6721 cgaaacatcg tagccggact gccttagttc ggcgagcacg cccaaaaagt cctcccagag
     6781 gaaatgcggc agcatgaagc gatcgtgcag cgtcgtgccc cagcgcacga agttgccttg
     6841 ctgcggctcg cgccagagct ttgcgatcag cgcccggatc aacagctgtt gcgccagaga
     6901 catgcgcgga tcgggcggca tttccaaagc gcggaattcg accagcccga gccggccggt
     6961 ggtgccatcg ggcgaataga gtttgtcgat gcagatttcg gcgcggtggg tgttgccggt
     7021 gatgtcaacg aggatatgcc ggaacagccg gtcgaccagc cacagcgggg ctttgatgcc
     7081 aggcggcggc acatgggcca gcgcgatctc cagttcataa aggccatcat gccgggcctc
     7141 gtcgatgcgc ggcgcctggc tggtggggcc gatgaacatg cccgagaaca aataggacag
     7201 cgacggatgc cgctgccaat acagcaccag gctcttcaag agatcggggc ggcgcaggaa
     7261 cggcgagtcc gccggcgtgc tgccgccgac caccacgtga ttgccgccgc cggtgccggt
     7321 gtgccggcca tcgacgagaa accggttggc gccgagccgg acttgcgcgg cgtcctcgta
     7381 gaggccgaac gtgatgtcga cggcttcgcg ccagttttgc gccggctgta tgttgatctc
     7441 gatcacgcca ggatcgggcg tcaccttgat cacctcgacg cgcgggtcga acggtggtgg
     7501 atagccctcg acatggacct gcatctgcat ttcttcggcg gttgcctcca ccgccgtgac
     7561 cagttcgaga taatcctcaa gcttctccac cggcggcatg aaggcgcaca aaatgccgtc
     7621 gcggatctcg atcgacatcg cggtgcggat gccgccgcct ttgagctgct gctcctgcac
     7681 ttcctgatgc ggcgcgggtt cgaccagcgc tgtcgcgtcg taaaccggca aggcgtcgcg
     7741 cggctccatc ggatcctgct cgacgacata gggaaattct tccgcgggaa tgtgaggcag
     7801 tgaggcgatc ggcagtcgca gtcccagcgg ggaatcgcct ggcgtcagaa acaggttgcc
     7861 gcgccgcagc ttccagcgct cgctcttcca gcgcgaggaa gatttgccgg catcggcgtt
     7921 ccagcgctgg atcggcagaa cgaatccctt gggaacgttg agcccctggt cgaacacccg
     7981 cgccatgcgc gagcgttctt ccggatccga cagcttggaa tcgcttggat cgacattggg
     8041 cggcaacgcg gcttctttct gcagccagtg cgagggatcc tcgaacgccg gcatgatgaa
     8101 ctcggaatcg acgccgagtt ttttcgccgc cccctccgcg aagcgctcgg cgtctgcaat
     8161 gccggctttg cgcggccctt cgatcctcgc gatcagatcg gagctcttcc agatcggcac
     8221 gccgtccttg cgccaataga gtccgaatgc ccagcgtggc aggctctcgc cggggtacca
     8281 tttgccctgt ccgaaatgta acaggccgcc cggcgcaaac cgcgcgcgca gcttgcgaat
     8341 gagatcgtcg gcaaggccgc gcttggtcgg gccgaccgcg gcgatattcc attccggcga
     8401 ttccagatcg tcgacggaaa cgaaagtcgg ctcgccgccc atggtcaggc gcacgtcgcc
     8461 ggccttcagg tcggcatcga cctgctcgcc gagctggtcg aggcgcgccc aggattcgtc
     8521 ggagaacggt tttgtaatgc gcggcgcctc gcggatccgc ttgacgctca tctcgaagcc
     8581 gaattcgacc tcggcgaaac cgaccgcgcc gctgatcggc gccgccgagc ggtaatgcgg
     8641 cgtggcggcg acgggaatat gcccctcccc ggtcagcatg cccgaggtga catcgaatcc
     8701 gatccacccc gcgcccggaa gatagacttc ggcccaggcg tgcaaatccg tgaaatcgtt
     8761 ctcgacttcg cgcggtcctt cgaccggatc gatgtcgggc cgcagctgga tgagatagcc
     8821 cgagacgaag cgggcggcga gcccgaggtg ccgaaagatc tgaatcagta gccacgccga
     8881 gtcgcggcac gatcccgcgc ccaactccag cgtctgctcc ggcgtctgga tgcccggctc
     8941 catccggatg atgtagcgaa tcttcttttg cagttgcgca ttgagatcga ccagaaaatt
     9001 gacggtgctc gccgcctcgc gcgggatgtc ggccagatac tttgccaaca acggcccagg
     9061 ctcgatggtc gccagatacg gagcgagttc ggtcttgaga tcgttggtgt attcgaacgg
     9121 aaagctgtcg gcggaaggct cgacaaagaa atcgaacgga ttgatcacgg tcatcgacgc
     9181 cgtgaaatcg acctcgatct tcagctcggt ggccttttcg ggaaagacaa agcgcgccag
     9241 ccagttgcct tgcgggtcct gttgccagtt cacgaaatgg ttcgaaggcg taaccttgag
     9301 cgaatagctc aggatcggcg tgcgcgtgtg cggcgccggc cgcagccgga cggtttgcgg
     9361 gccgaggtcg atcggacggt catatttgta atgcgtgacg tgatgtagtg cgacgaagat
     9421 cgacacaggg cgatggctcc agcggctttt ttaagcagaa caccggttcc gatggggatc
     9481 aagcactaac aacgggcagc tgatgcctat gtgaggtgcg gaagtaaagg cgaatagcga
     9541 gtggcgaata gggaatagtt ccattcgcca ctccctattc gctattcgct cacttacatc
     9601 gtcgcgccga gcacccatgg cgcgaattcg gcgccgccga aatcaaagct ctcgctcttg
     9661 gtcggctggc cagaggcggt cttgaggatc aggtcgaaaa tccgctggcc gcacgcctgc
     9721 accatttcct cgccgtcgag aatggtgccg caattgacgt ccatgtcgtc ttccatgcgc
     9781 ttgaacatcg cagagttggt cgcaagcttg atcgatggcg cgggcttgca gccgaacacg
     9841 ctgccgcggc cggtggtgaa gcagacgagg ttggcgccgc ccgccacctg cccagtcgcc
     9901 gcgaccgggt cgtagcccgg cgtgtccatg aagacgaaac cctttttggt gatctgctcg
     9961 gcgtagttca cgacatcgac gaggttggta ctgccggcct tggccatcgc gccgagtgat
    10021 ttttccagga tcgtggtaag cccgccggcc ttgttgccgg gagagggatt ggcattcatc
    10081 tcggcgccct cgcgcgccgt gtattcctcc caccagcgca tcagcgccac cagtttttcg
    10141 ccgacctcgc ggctgaccgc gcgtcgcgtc aacagatgtt cggcgccgta ggtctcgggc
    10201 gtctccgata aaatgacagt gccgccgtgc cgcaccaaga gatcgctggc agcacccagc
    10261 gccgggttgg cggagacgcc ggaatagccg tcggagccgc cgcattgcag cgccacggtc
    10321 aactcgctgg ccgacaccgg ttcgcgcttg acgttgttgg cgtcggtgag cgcctcgcgc
    10381 acgaaggcga cgccggcttc gacggtcttg cgggtgccgc cgatttcctg aatttccagt
    10441 tcgcgcaggc ggccggcgag tttttgttcc ttcatcaacc caccgatctg gttgacctcg
    10501 cagcccaggc ccagcacgac
//
LOCUS       DATNOI010000015         3183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2308593,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000015 DATNOI010000000
VERSION     DATNOI010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2308593"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VN968_00540"
     CDS             <1..138
                     /locus_tag="VN968_00540"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473149.1"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the delta' subunit seems to
                     interact with the gamma subunit to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HXL27770.1"
                     /translation="NLPRLARLAEVWEKINRAARDTAEYNLERKPLVFSVFGLLAEAI
                     G"
     gene            157..1767
                     /gene="metG"
                     /locus_tag="VN968_00545"
     CDS             157..1767
                     /gene="metG"
                     /locus_tag="VN968_00545"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HXL27771.1"
                     /translation="MYHHTSFEDRKAFAIMANATNLHREPYYITTAIAYPNGVPHIGH
                     AYEAIATDALARFARLDGKDVFFLTGTDEHGLKMIQTAEAEKLPTLEVATRNAQRFKD
                     MDERLNVSFDRFIRTTEEQHHRSSQELWKRMADNGDIYLDSYAGWYSVRDEAYYAEDE
                     TVLGEDNVRRGPQGTPVEWVEEKSYFFKLSAYQDKLLALYESQPDFIEPDSRKNEVVS
                     FVKSGLRDLSISRTTFDWGVKVPGDPEHVMYVWVDALTNYITGVGFPDESDPNWRYWP
                     ADVHIIGKDIIRFHAVYWPAFLMSAGIPVQKRVYAHGFLFNKGEKMSKSVGNVVDPFN
                     LANQYGVDQMRYFFLREVPFGQDGNYNHEAIVARINADLANDLGNLAQRSLSMIAKQY
                     DGVLPEPGAFTANDKAILAQADGMVGLARAAMATQQIHQALNAVWAVVAEANRYFAGE
                     APWALAKTDPQRQKTVLYVTSEVIRQIAILAQPVMPESSARLLDSLGIPQAARDFATL
                     GGAARIKPGTVLPAPAPVFPRYVEPSAA"
     gene            1782..2561
                     /locus_tag="VN968_00550"
     CDS             1782..2561
                     /locus_tag="VN968_00550"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXL27772.1"
                     /translation="MLVDSHCHLDFPDFADDLDAIVARAAAAGIGRIVTISTRVRRLG
                     ELLAIAERFPNVYCSVGTHPHHADEEDGVLADELIGLTRHPKVVALGEAGLDYFYQHG
                     SPQAQERGFRAHIAAARATGLPLVIHTRDADDDCGRILEDEMSKGSFRAVLHCYTGGR
                     ELAMKAISLGLSISFTGILTFKKSQSLRDLAAELPADRIMVETDAPYLAPGKFRGKRN
                     EPAYVVEIAKVLAETRGVSLEEISRQTTQNFFRLFSKVPAA"
     gene            2558..>3183
                     /locus_tag="VN968_00555"
     CDS             2558..>3183
                     /locus_tag="VN968_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL27773.1"
                     /translation="MTLTLTILGCGSSAGVPRPALGWGACDPKNPKNRRRRCSLLVER
                     TGERGVTRILIDTSPDLREQLIEAKVDHIDAVFLTHEHADQTHGIDDLRSVVLHQRRR
                     IPVYLSQSTAKDIMHRFSYCFVSAPGSAYPPILNQHSIEGGESQTIEGKGGAVLISAF
                     LLRHGNIPALGYRIGDAAYTPDVNDIPRESWPHLENLDLWIIDGLRYTG"
BASE COUNT          630 a          992 c          949 g          612 t
ORIGIN      
        1 aacctgcccc gccttgcacg gctggcggag gtgtgggaaa agatcaatcg cgccgcgcgc
       61 gataccgcag aatataatct cgagcgaaaa ccgctggttt tctcggtgtt cgggctcctc
      121 gcggaggcga tcggctgaca ggccggttta gccagtctgt atcaccacac gtcgtttgaa
      181 gatcgaaagg ccttcgcaat tatggcgaac gcaaccaatt tgcaccgtga gccctactat
      241 atcacgaccg ccatcgccta tccgaacggc gtgccgcata tcggccacgc ctatgaggcg
      301 atcgcgaccg atgcgctggc gcgctttgcg cggctcgacg gcaaggatgt gttctttctc
      361 accgggaccg acgaacacgg cttgaagatg atccagaccg cggaagccga aaaactgccg
      421 acgctggaag tcgcgacacg caatgcccag cgattcaagg acatggatga gcgcctgaat
      481 gtctcattcg accgattcat ccgcaccacg gaagaacaac atcatcgctc cagccaggaa
      541 ctatggaagc ggatggccga taacggcgac atctatctgg acagttatgc cggctggtac
      601 tccgtgcgcg acgaagccta ctatgccgaa gacgaaaccg tcctgggcga ggacaatgtg
      661 cggcgcgggc cgcagggcac gccggtggaa tgggtggagg agaagagtta tttcttcaaa
      721 ttgtccgcct atcaggacaa gcttctggcg ctctacgaga gccagccgga tttcatcgag
      781 cctgactcgc gcaaaaacga agttgtcagc ttcgtcaaga gcggattgcg ggatctgtcg
      841 atttcgcgga ccacattcga ttggggcgtc aaggtgcccg gcgatcccga gcacgtcatg
      901 tatgtctggg tcgacgcctt gaccaactac atcaccggcg tcggctttcc cgatgaaagc
      961 gatccgaact ggcgctactg gccggccgat gtgcacatca tcggcaagga catcatccgc
     1021 tttcacgccg tctactggcc ggccttcctg atgtcggcgg gcattccggt gcaaaagcgc
     1081 gtctatgccc acggctttct gttcaacaag ggcgagaaga tgtcgaaatc ggtcggcaat
     1141 gtcgtcgatc ccttcaatct cgccaatcaa tatggcgtcg atcagatgcg ctacttcttc
     1201 ctgcgcgagg tgccgttcgg gcaagacggc aattataatc acgaagccat tgtcgcccgc
     1261 atcaatgccg atctcgccaa cgatctcggc aatctggcac agcgctcgct gtcaatgatc
     1321 gcgaagcaat atgacggcgt gttgccggag ccgggtgcct tcaccgccaa cgacaaggca
     1381 atcctcgcac aggccgacgg catggtcgga ttggcgcggg ccgcgatggc gacccagcag
     1441 attcatcagg cgctcaacgc cgtctgggcg gtcgtggcgg aagccaaccg ctatttcgcg
     1501 ggcgaggcgc cgtgggcgct ggcgaagacc gatccgcaac gccagaaaac cgtgttgtac
     1561 gtgacatcag aggtcatccg gcagatcgcc attctggcgc agcccgtcat gccggaatct
     1621 tcggcgaggt tgctggatag cctcggcatc ccacaagccg cgcgcgactt cgccacgctg
     1681 ggcggcgcag cgcgtatcaa gccgggtacg gtgctgcccg caccggcgcc ggtgtttccg
     1741 cgttacgtcg aaccatcagc ggcttgaact gttggaatgc gatgctggtc gatagccact
     1801 gccacctgga ttttcctgac ttcgccgatg atctcgacgc catcgtggcc cgggcggcag
     1861 ctgcagggat cgggcgcatc gtcaccatct cgacccgggt gaggcgcctc ggcgagctcc
     1921 ttgcgatcgc cgagcggttt ccaaacgtct attgctcggt aggcacccat ccgcaccacg
     1981 ccgatgagga agacggagtg ctcgccgacg aactgatcgg gctgacgcgg catcccaagg
     2041 tcgtggccct gggcgaggcg gggctggatt atttctacca gcacggctcg ccccaagccc
     2101 aggagcgcgg ctttcgcgcg cacatcgccg ccgcccgcgc gaccggcctg ccgctggtca
     2161 ttcacacccg cgacgccgac gacgactgcg gccgcatcct cgaagatgag atgagcaaag
     2221 gatcatttcg cgccgtgctg cattgttaca ccggcgggcg cgagctggcg atgaaagcaa
     2281 tctccttggg tctgtcgatt tcattcacgg gcattctgac gttcaagaaa tcgcaaagcc
     2341 tgcgcgatct ggcggccgag cttccggccg accgcatcat ggtcgagacc gacgcgccgt
     2401 atctggcgcc cggcaagttt cgcggcaagc gcaacgagcc ggcttacgtg gttgaaattg
     2461 ccaaggtgct ggcggagacg cgtggagtct cgctcgagga gatatcgcgt caaaccacac
     2521 agaatttctt ccgcctgttc tctaaagtac cggctgcatg acgctgacgc ttacaattct
     2581 tggctgcgga tcctccgccg gcgttccgcg tcccgcgctc ggctggggtg cgtgcgatcc
     2641 caaaaatccg aaaaaccgcc gccggcgctg ctcgctgctc gtcgagcgta cgggggagcg
     2701 cggcgtcacg cgaatcctga tcgatacctc gcccgatctg cgcgagcagt tgatcgaggc
     2761 caaggtcgat cacattgatg cagtgttcct gacccatgag catgccgacc agacccacgg
     2821 catcgacgat ctccgttccg tcgtgctgca tcagcgccgg cgcattccgg tctatttgag
     2881 ccagtcgacc gcgaaagata tcatgcaccg gttttcctat tgctttgtgt cggcgccggg
     2941 cagcgcttat ccgccgatcc tcaaccaaca ttcgatcgaa ggcggcgaga gccagaccat
     3001 cgaagggaag ggtggcgctg tcttgatttc cgccttcctt ctccggcacg gcaatatccc
     3061 ggcgctcggt tatcgcatcg gcgacgccgc ctatacacct gatgtcaacg acatcccgcg
     3121 cgagagttgg cctcatctcg aaaatctcga tctctggatc atcgacgggc tgcgctatac
     3181 cgg
//
LOCUS       DATNOI010000016         5052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2308437,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000016 DATNOI010000000
VERSION     DATNOI010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2308437"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VN968_00560"
     CDS             complement(<1..565)
                     /locus_tag="VN968_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HXL27774.1"
                     /translation="MRVGVPKEIKVQEYRVGLTPGTAREYVAAGHSVVVETNAGAGIG
                     ATDEAYRKVGAKIADTAAEIFASADMIVKVKEPQAGEWSRLREGQILFTYLHLAPDPE
                     QTKGLIDSGCTAIAYETVTSPRGGLPLLAPMSEVAGRMSIQVGAHCLEKAQGGAGILL
                     GGVPGVAPARVVIIGGGVSGTHAARMAV"
     gene            700..1203
                     /locus_tag="VN968_00565"
     CDS             700..1203
                     /locus_tag="VN968_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXL27775.1"
                     /translation="MKIDSFDTTILSELTVNARASHVELAERVGLSSTAIARRQKVLE
                     EEGFIRGYQAVLDLTRFGLSTTVLVRVTLESQNEDALKSFEAAVIGCPSVVRCFLMSG
                     SDDYIVIVLARDIEDFERIHRTELSRLPRVARIQSSFAMREVVNRAVPPVIFGGSRRP
                     RSAQAAR"
     gene            complement(1188..1982)
                     /locus_tag="VN968_00570"
     CDS             complement(1188..1982)
                     /locus_tag="VN968_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL27776.1"
                     /translation="MQLSISGVETFVATGGRDFASSLPTIVLLHGAGFDHTTWALHSR
                     WFAHHGYGVLAPDLPGHGRSSGAPLQAIVDMADWTAALLDAAGVAKAWLVGHSMGSLI
                     ALEAAARHPAKVSALGLIGTAATMTVGPDLLKAAEANDHSAVDMVSIWGLGFQAGLGG
                     SLAPGLWMHSGAQRVLEQCRPGVLFNDLAACNAYQDALAAAARVTVPATFILGERDLM
                     TPAKAGKALASALPNSRTVVIPGAGHMMMVERPDELLAALQGYRAA"
     gene            complement(1986..3281)
                     /locus_tag="VN968_00575"
     CDS             complement(1986..3281)
                     /locus_tag="VN968_00575"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HXL27777.1"
                     /translation="MPTPKPPAFETLSLHSGQHPDPVTGARAVPIYQTTSYVFQDSEH
                     AAALFNLERAGHIYTRISNPTTAVLEERLAALEGGVGAICTASGMAALHLGIATLLGA
                     GDHIVASSSLYGGTINLLAHTLPRFGITTTFVKPRALDEFAAAIKPNTRLLIGETIGN
                     PGLEVLDIPNVARIAHDAKTPLLIDNTFATPYLSRPIELGADIVMHSATKWIGGHGIA
                     IGGVIVDGGRFDWRLSGKFPVLTEPYAGYHGIVFDEQFGPSAFIMRARTEGLRDFGAC
                     LSPTNAFQLLQGVETLGVRMDRHIENTHAVLEFLTASKAVDWVLHPSLENHPDHVLAR
                     QLLPRGAGSIISFGIKGGRPAGKKFIESLRLISHLANVGDAKTLVIHPASTTHQQMDA
                     AQLKASGIGEELVRLSVGIESASDITDDLGQALRLSQKA"
     gene            3476..3925
                     /locus_tag="VN968_00580"
     CDS             3476..3925
                     /locus_tag="VN968_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27778.1"
                     /translation="MSGAKGSVIWRATVAMFALALLSAPAGPAVAAEKWDLYVYNPVS
                     TVAAVKGLNTVIEQIEKETGGELSIRLHLGGSLPINTTTITQAVSDDVVQMGDDGYFL
                     GNVPIGGVLRLPMLLRTLADYDKAAAIMKPYLEKASTSFQITDEVCG"
     gene            3932..4117
                     /locus_tag="VN968_00585"
     CDS             3932..4117
                     /locus_tag="VN968_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27779.1"
                     /translation="MALTFLSMSVAEAHGAFHRVELVQTRLSFAARLILQMVFDFISL
                     VASALVTWQLYRLAANS"
     gene            4264..4854
                     /locus_tag="VN968_00590"
     CDS             4264..4854
                     /locus_tag="VN968_00590"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXL27780.1"
                     /translation="MTPGVELIAVMLVFLGLLTAGMTIPFAILVPSVLYLVMHAGLAG
                     LKGIGLVSWGSMNSFTLTAIPLFILMAEILQESKLSQRVYRGLSTLVAWMPGGLLQTN
                     IAGCAIFAAISGSSVVTAASIGRVALPELQRRNYSPRLSAGSLAAGGTLGILFPPSIA
                     MIVYGTFTETSVAKLWGWSRRRKQRRSDACWPWRSP"
     gene            4788..>5052
                     /locus_tag="VN968_00595"
     CDS             4788..>5052
                     /locus_tag="VN968_00595"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXL27781.1"
                     /translation="MGLVTPTEAAAVGCLLAMAIAMICGEFNFSVLKAALQTCVRVCG
                     NILFIVYAAFVFSYAISYAGVGEQITRFLVDLKLSRLEFFLALF"
BASE COUNT          905 a         1514 c         1663 g          970 t
ORIGIN      
        1 ccaccgccat gcgtgcagca tgggttccgg agacgccgcc gccgatgatc accacccgcg
       61 ccggcgccac gccggggacg ccgcccagga ggatgccggc acccccttgc gccttttcca
      121 ggcaatgggc gccgacctga atcgacatgc gtcccgcgac ctcgctcatg ggcgccagaa
      181 gcggcaggcc gccgcgcggg ctcgtcaccg tctcataggc gatggcggtg cagccggagt
      241 cgatcagccc cttggtctgc tcgggatcgg gcgccagatg cagataggtg aagaggatct
      301 ggccctctcg gagccgggac cattcgccag cctggggctc tttgaccttc acgatcatat
      361 cggcgctggc gaaaatctcg gcagcggtgt cggcgatttt agctccgacc ttgcgatagg
      421 cctcatcggt ggctccgatg cctgcgcccg cgttggtctc gacgacgacg ctgtggccag
      481 cggcgacata ttcacgagcc gtgccaggtg tgagaccgac gcggtactcc tggaccttga
      541 tttccttcgg tactccgaca cgcatcatgg aatctccctg ttttgaaggc aggtttctag
      601 gtcagatacc ggtttgaatc cccgcgatct tgatgcatta atctgaattc tcgccggaag
      661 ccggcatgat ccggacactt cgcgcaggaa tcggaacgca tgaagatcga tagcttcgat
      721 acgaccatcc tctctgaact cacggtcaac gcccgggcca gccacgtcga actcgctgag
      781 cgtgtcggac tttcgagcac ggcgatcgcg cggcggcaga aggtgctgga ggaagaaggt
      841 ttcatccgcg gctatcaggc ggtactggat ctgactcggt tcgggctgtc caccaccgtt
      901 ctggtcaggg tcacgctgga aagccaaaac gaggacgctc taaaatcctt cgaagccgcg
      961 gtgatcggct gtccatcggt ggttcgatgc ttcctgatgt cgggcagcga cgactacatc
     1021 gtcattgtcc tggcgcgcga tatcgaggat tttgagcgca tccaccgaac cgagctctcg
     1081 cgattgccgc gggttgcgcg gattcaatcg agtttcgcca tgcgcgaggt cgtgaaccgg
     1141 gcggtcccgc cggtgatttt tggtggatcg cgacgacctc ggtcggctca ggcggcgcga
     1201 tagccttgca gggccgcgag cagttcgtcc ggccgttcga ccatcatcat gtggcccgcg
     1261 cctggtatca ccacggtgcg cgaattcggc agcgccgatg caagcgcctt gccggccttc
     1321 gccggtgtca tcaagtcgcg ttcgccgaga atgaacgtgg ccggcaccgt aacccgcgcg
     1381 gcggcggcga gcgcgtcctg ataggcgttg caggcggcaa gatcgttgaa cagcacaccg
     1441 gggcggcatt gttcaaggac ccgctgggcg ccggaatgca tccacaatcc cggcgcgagg
     1501 ctgccaccca gcccggcctg aaatccaagg ccccagatcg aaaccatgtc gacggcggaa
     1561 tgatcgttgg cttcggcggc cttgagcaga tcgggtccca cggtcatggt cgccgccgtg
     1621 ccaatcaggc cgagcgccga gactttggcc gggtgccgcg ccgcggcctc gagtgcgatc
     1681 aacgatccca tggaatgacc gaccagccat gcctttgcca cgccggcggc atcgagcagc
     1741 gccgcggtcc agtccgccat gtcgacgatt gcctggaggg gcgcgccgga tgaacggcca
     1801 tggcccggca aatccggagc cagcacgccg tagccgtgat gcgcaaacca ccggctgtgc
     1861 agggcccagg tggtatggtc gaagccggcg ccgtgcagaa gcacgatggt tggcagcgag
     1921 gaagcgaagt cccggccacc ggtcgcgaca aaggtttcaa cgccactgat ggacaactgc
     1981 atggctcaag ccttctgcga taggcggagc gcctggccga ggtcgtcggt gatgtcggag
     2041 gcggattcga tgccgaccga cagccgaacc agctcttcgc caattccgga ggctttgagc
     2101 tgggctgcat ccatctgctg atgggtggtg ctggcgggat gaatgaccag cgtcttggcg
     2161 tcgccgacgt tggcgaggtg gctgatcaac cgcaagctct cgatgaattt cttgccggca
     2221 ggccggccgc ccttgattcc gaagcttatg atcgaccccg cgccgcgcgg cagcagttgc
     2281 ctggccagca catgatccgg atggttttcc agtgaaggat gcagaaccca gtctaccgcc
     2341 ttgctcgcag tcaggaattc cagaaccgca tgggtatttt cgatatgacg gtccatgcgg
     2401 acgccgaggg tctcgacacc ctgcagcaat tgaaaggcgt tggtcggcga gaggcaggcg
     2461 ccgaagtcgc gcaggccctc ggtgcgcgcc cgcatgatga acgccgatgg gccgaattgc
     2521 tcgtcgaaaa cgatgccgtg atagccggca tagggctcgg tcagcaccgg aaattttccg
     2581 gaaagccgcc aatcgaaccg gccgccatcg acaatcacgc cgccgatggc gatgccgtgg
     2641 ccgccgatcc atttggtggc ggagtgcatc acgatatcgg cgccaagttc gatcgggcgg
     2701 ctgagatagg gtgttgcgaa ggtattgtcg atcagcaatg gagtttttgc atcgtgggcg
     2761 attcgcgcga cgttggggat atccagcact tcaaggccgg gattgccgat ggtctcgccg
     2821 atcagcaggc gcgtgttggg tttgatcgcg gcggcgaatt catccagcgc gcgcggcttg
     2881 acgaaagtcg tggtgatgcc gaaacgcggc aacgtgtgcg ccagcagatt aatggtgccg
     2941 ccgtaaagtg acgaggacgc cacgatatgg tcgccggcgc caagcagcgt ggcaatgcca
     3001 agatgcagcg ccgccatgcc gctcgccgtg cagatggcgc cgacgccgcc ctcgagtgcg
     3061 gccagacgct cctcgagcac cgcggtcgtc gggttcgaaa tccgggtgta gatatggccg
     3121 gcacgctcca ggttgaacag cgccgcggcg tgctcggaat cctgaaacac gtaggacgtg
     3181 gtctgataga tcgggacggc tcgcgcgccc gtcacgggat ccggatgctg tcccgaatgc
     3241 aggctgaggg tctcgaaagc gggcggcttg ggtgtgggca tcgcggctcg caaatgatga
     3301 gcagatgatc tgaacgcaca ggtccggaag accgccggat gcccgcgtcg tatgccacaa
     3361 acccgagcgg aatgttagtg ccggcgcgca tgggagcaag tttttaccgg cgcttgcccg
     3421 cctcgcagcg atcgaggata ttgaaaaaaa cagaaggcta gagggagcga aaacgatgtc
     3481 cggggccaaa ggcagtgtca tctggcgggc gacggtggcg atgtttgcgt tggcgcttct
     3541 gtcggcgccg gccgggccgg cagtggcggc cgaaaaatgg gatctctacg tctacaatcc
     3601 cgtatcgacg gttgcggccg tcaaaggcct gaacacggtc atcgagcaga tcgaaaagga
     3661 aaccggcggc gaattgtcga tccgcctgca tctcggaggt tcgctcccga tcaacaccac
     3721 caccatcacc caggccgtca gcgacgacgt cgtgcagatg ggagacgatg gatattttct
     3781 cggcaatgtg ccgatcggcg gcgtgctgcg gctgccgatg ctgctgcgga cgctggcgga
     3841 ttacgacaag gccgcggcca tcatgaagcc ttatctggaa aaggcatcga cgtcgtttca
     3901 gatcacggat gaagtctgcg gctaactgct ggtggcgctg acatttttga gcatgtcggt
     3961 cgccgaggcg cacggcgcgt tccatcgcgt cgagctggtt cagacccgtc tgtccttcgc
     4021 ggcgcggctg atcttgcaaa tggtgttcga tttcatctcg ctggtggctt ccgcgctggt
     4081 cacctggcag ttgtaccggc ttgccgccaa ttcgtgacgc gccgaagacg tcgcgccgac
     4141 gccactgcag acgccgttgt ggctgccgca aagcgcgatg gcgatcggga tgtcgctgct
     4201 gtgcttggcg ctgatacgga ccatggtgac caaggcgagg agtttgcgca ggggtgatcg
     4261 atcatgacac caggcgtcga attgattgcc gtgatgctgg tcttcctcgg cctgctcacg
     4321 gcgggaatga ccattccgtt cgcgattctg gtcccgtcgg tgctctatct cgtgatgcat
     4381 gccggccttg cgggactgaa aggcatcggg ctggtcagct ggggcagcat gaacagcttt
     4441 accctgacgg cgatcccgct gttcatcctg atggccgaga ttcttcagga gagcaagctc
     4501 agccagcggg tctatcgcgg cttgtcgacc ctggtcgcct ggatgcccgg cgggctgctg
     4561 cagaccaata tcgccggctg cgccattttc gccgcgatca gcggatcgag cgtggtgacg
     4621 gcggcgtcga ttggccgcgt tgcgctgccg gaattgcagc ggcgcaatta cagtccgcgg
     4681 ctgtcggctg gttcgctggc cgccggcggg acgctcggga ttctgtttcc gccgagcatc
     4741 gcgatgatcg tctatggcac gttcaccgaa acctcggtgg cgaaactgtg gggctggtca
     4801 cgccgacgga agcagcggcg gtcggatgcc tgctggccat ggcgatcgcc atgatctgcg
     4861 gtgaattcaa tttcagcgtc ctcaaggcgg cgctgcagac ctgcgtccga gtctgcggca
     4921 acatcctgtt catcgtctat gccgcgttcg tgttttccta cgccatctcc tatgccggcg
     4981 tcggcgagca gatcacccgg tttctggtgg atctcaaact gtcgaggctc gaattctttc
     5041 tggcgttgtt tg
//
LOCUS       DATNOI010000017         7248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_881319,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000017 DATNOI010000000
VERSION     DATNOI010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881319"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VN968_00600"
     CDS             <1..295
                     /locus_tag="VN968_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXL27782.1"
                     /translation="QAAQLQSKMQAMQEELGHVEVEGISGGGLVTVRMTAKMQVKAVK
                     IDPSLMKADEREILEDLLVTAHNDAQRKAEAAMQEKMQALTGGLGLPPGLGLT"
     gene            297..902
                     /gene="recR"
                     /locus_tag="VN968_00605"
     CDS             297..902
                     /gene="recR"
                     /locus_tag="VN968_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXL27783.1"
                     /translation="MAAAVAGPEIERLIQLLARLPGLGPRSARRAALHLIKKREALMT
                     PLSAALQVAIDKIQVCKTCGNIDTQNPCTVCTDPRRDPAIIVVVADVADLWALERAHA
                     TNGFYHVLGATLSPLDGVGPQDLTIDALVARAHDPRVTEIILALNATVDGQTTAHYIT
                     DLLQEAGVKVTRLAHGVPVGGELDYLDEGTLSAAMRQRTLF"
     gene            923..1354
                     /locus_tag="VN968_00610"
     CDS             923..1354
                     /locus_tag="VN968_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27784.1"
                     /translation="MSRVRFGRQLALAAFMAATLAPPAVRAQQAEPAPKPGKMINAGD
                     VLSGELNTLRMRGGKKGKAAATYQLTSEPRRLPPPNGLCNLETGPETFQLITSSEAQA
                     AQLKGFIGKEISVKVGEIACAQDAGQMSEAVVTNWSVVTKH"
     gene            complement(1541..2911)
                     /locus_tag="VN968_00615"
     CDS             complement(1541..2911)
                     /locus_tag="VN968_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL27785.1"
                     /translation="MNTPEAPSSSPKESAKPRASWREGLAVYLQRRVLIVLLLGFSSG
                     LPLALSGSTLQVWMRESGVDLGTIGLLALVGTPYTLKFLWAPLVDALHVPFFTRAFGR
                     RRGWLLFSQLLLIGTILLLALTDPARSPLFAALGALLVAAASSTQDIVVDAFRVESLP
                     ESEQAAGMASYVAAYRIGMLVSTAGALFIVSGFESTGIARSSAWMWGYVAMAALVLIG
                     IVTALAATEPGQSARAEAATSADSAFTRVIHAAVGAFSEFLARKDALAALAFVVLFKF
                     TDAFSGTMTAPFVIDIGFSKVDYAAIVKGVGLAATLIGGFAGGFIARRYSLALSLWIG
                     GVLQAVANLSFSWLAMVGVNQWALAFAITAENFTSAIGTVIFVAYLSALCKNPLHTAT
                     QYALLTALAAVGRTYLSSGAGYVAKATGWPLFFAICVMVAVPSLILLAWLERRGHFAT
                     LEPVRV"
     gene            complement(3175..4383)
                     /gene="rmuC"
                     /locus_tag="VN968_00620"
     CDS             complement(3175..4383)
                     /gene="rmuC"
                     /locus_tag="VN968_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HXL27786.1"
                     /translation="MNEILFVMGGLPVRTGSALIGFGALALVLLLSIAIVIARSGRRG
                     AELAMAQAIRADELEERLSEMLRAQSEATGRVDAMGQQLAGRQADMARAVNERLDSVT
                     HRVGQSMEQTTRNTMDSLRVLHERLGIIDNAHKNLTDLTSQVTTLRDVLANKQSRGAF
                     GQARMEAIVQDGMPKGSYEFQFTLSTGKRPDCVVFLPDQRPLCIDAKFPLEAMTALHD
                     ARTDEEKKFATQRLRADVLKHVSDIAEKYLIAGETQDTALMFVPSESVYAEIHDGFDD
                     VIQKAYRARVVLVSPSLLMLAIQVMQQILKDARMRDAADLIRTEVLNLGDDLNRLRDR
                     VLKLQNHFGQANEDVRQILISADKIEKRAGRIEELDFSKSEAPPETPRLVKSAAPELF
                     PLPRQLQAGE"
     gene            4556..5083
                     /gene="def"
                     /locus_tag="VN968_00625"
     CDS             4556..5083
                     /gene="def"
                     /locus_tag="VN968_00625"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXL27787.1"
                     /translation="MAIREIIILPDKQLRLISKPIEKVTAEVRKLADDMFETMYAAPG
                     IGLAAIQVAQPLRLITMDLAKRDEDGESKPRPRVFINPEILSSSEELSVYEEGCLSIP
                     EYYEEVERPARVRVRFTDLDGKVHEEEAEGLFATCIQHEIDHLNGVLFVDYLSKLKRD
                     RVLKKFAKAAKRAAE"
     gene            5223..6155
                     /gene="fmt"
                     /locus_tag="VN968_00630"
     CDS             5223..6155
                     /gene="fmt"
                     /locus_tag="VN968_00630"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXL27788.1"
                     /translation="MPLRLIFMGTPDFAVPTLLELVAHGHEIAAVYTRAAKPAGRGMK
                     LQPTPVEVQARRLGIPVATPATLKTPEALKAFSAHHAEAAVVVAFGMILPQAILDTPK
                     LGCFNLHASLLPRWRGAAPINRAIMAGDPETGVMVMKMAAGLDTGDVAMAERMAITDA
                     MTAADLHDALAPLGADLMVRAMGALERGRLQLTKQSEDGVTYAAKIEKAEARIAWNKP
                     ARAVLRHIHGLSPFPGAWCEVASESEQVRVKILRCEMIDRSGAPGDVLDDHLTIACQQ
                     GALRILELQRAGKAPMKAADFLRGTPLKAGARFA"
     gene            6328..7065
                     /gene="truA"
                     /locus_tag="VN968_00635"
     CDS             6328..7065
                     /gene="truA"
                     /locus_tag="VN968_00635"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HXL27789.1"
                     /translation="MPRYKLTIEYDGAPFCGWQIQDNGASVQGALETAITAICGEQVR
                     VHGAGRTDAGVHALAQVAHCDIAKHFVPGRFRDGLNAHLRPHPIGVLSAEIVPDTFEA
                     RFSAKKRHYLYRISNRRANLALEIGHVWRLPRPLDTDAMQAAAQRLIGKHDFTTFRDT
                     ECQAKSPEKTLDQLDVVRLGDAVNIVTSARSFLHSQVRSMVGSLVWVGEGRWNADDLA
                     AALAARNRAACGPVAPPDGLYLVRVEY"
BASE COUNT         1382 a         2269 c         2372 g         1225 t
ORIGIN      
        1 gcaggcggcg caactgcaat cgaagatgca ggcgatgcag gaagaactcg gccatgtcga
       61 ggtcgagggc atctcgggcg gcggtctcgt cacggtgcgc atgacggcaa agatgcaggt
      121 gaaggcggtg aagatcgacc cctcgctgat gaaggcggac gagcgtgaaa tcctcgaaga
      181 cctgctggtc accgcccata acgacgcgca gcgcaaggcg gaggccgcga tgcaggagaa
      241 gatgcaggcc ctgaccggcg gtttgggtct gccgccgggg ctgggcctca cgtaatatgg
      301 ccgctgcggt tgccggtccc gaaatcgaac gcctgatcca gctgctggcg cggctaccgg
      361 ggcttggccc gcgctcggcg cggcgcgccg cgctgcatct gatcaagaag cgcgaagcgc
      421 tgatgacgcc gctctccgcc gcgctgcagg tggcgatcga caagatccag gtttgcaaga
      481 cctgcggcaa tatcgacacc cagaatccct gcacggtgtg caccgacccg cggcgggatc
      541 ctgcgatcat cgtcgtagtc gccgacgttg ccgacctgtg ggcgctggaa cgggcgcacg
      601 cgaccaatgg cttctaccac gtgctgggcg cgacgctgtc gccgctcgat ggcgtgggcc
      661 cgcaggatct gaccatcgac gcgctggtgg cacgcgccca cgacccgcgg gtgacggaaa
      721 tcattctggc gttgaatgcg accgtcgacg gccagaccac cgcgcattac atcaccgacc
      781 tgttgcagga ggcgggcgtc aaggtgacgc ggctcgccca tggcgtgccg gtcggcggtg
      841 aactcgacta tctcgatgaa ggcacgctgt ccgcggcgat gcgacagcgg acgttgtttt
      901 gaaaactgga atcagagggc acatgagcag agtaagattc ggcaggcaac tggctttggc
      961 ggcattcatg gcggcaacgc ttgccccgcc tgccgtgcgg gcgcagcagg ccgagcccgc
     1021 gccgaagccc ggcaagatga tcaatgccgg cgacgtgctg tcgggcgaac tcaacacgct
     1081 gcgcatgcgc ggcggcaaaa agggcaaggc ggccgccacc tatcagctca ccagtgagcc
     1141 gcgccggctg ccgccgccga acggactgtg caatctcgaa accggaccgg agacgttcca
     1201 gctcatcacc agcagcgagg cccaggcggc gcagctgaag ggctttatcg gcaaggaaat
     1261 ttcggtgaag gtcggcgaga tcgcctgcgc gcaggacgcc gggcagatga gcgaggccgt
     1321 ggtcacgaac tggagcgtgg tgacgaagca ttgacgatgc cgtcatcacg ggtaagcagt
     1381 catcacaagc aatgccgtca tcgcccggcc tgaccgggcg aaccagtatt ccggagcttt
     1441 gatgttcgaa catccacgct ctggaatact gggccacccg ctttcgcggg tggtgacggc
     1501 tgaaattgta gagcggcagt gcccctccgt taccgacgcg ctaaaccctc accggctcca
     1561 gcgtcgcaaa atgcccgcgc cgctccagcc acgcgagcag gatcaggctc ggcaccgcga
     1621 ccatgacgca gatcgcaaaa aacagcggcc agccggttgc cttcgccacg taacccgcgc
     1681 cggacgacaa ataggtgcgc ccgacagcgg caagtgcggt caagagcgcg tattgcgtcg
     1741 ccgtgtgcag cggatttttg cacagcgccg agagataggc gacgaagatc acggtgccga
     1801 tggcgctggt gaaattttcc gccgtaatgg cgaaggccag cgcccattga ttgacgccga
     1861 ccatcgccag ccacgagaac gacagattgg cgaccgcctg cagcaccccg ccgatccaca
     1921 ggctcaaggc cagcgaatag cgccgcgcaa tgaatccgcc ggcaaaaccg ccgatcaagg
     1981 tggcggcgag gccgacgccc ttgacgatcg cggcgtagtc aaccttgctg aagccgatgt
     2041 cgatcacgaa aggcgcggtc atggtgccgg aaaacgcgtc ggtgaacttg aacagcacca
     2101 cgaaggcgag cgccgcgaga gcatccttgc gcgcgaggaa ttcggagaag gcgccgaccg
     2161 cggcgtggat cacgcgcgtg aaggcgctgt ccgcgcttgt cgcggcctcc gcacgcgccg
     2221 attgtccggg ctcggtggcg gccagcgccg tgacgatgcc gatcagcacc aatgccgcca
     2281 tcgccacata accccacatc caggccgaag atcgtgcgat gccggtgctc tcgaacccgc
     2341 tgacgatgaa caacgcaccc gccgtcgaga ccagcatgcc gatccggtag gccgcgacat
     2401 aggacgccat gccggcggcc tgctcgcttt cgggcaggct ctcgacgcga aacgcatcga
     2461 ccacgatgtc ctgcgtcgat gacgccgccg cgaccagcag cgcgccgagc gccgcgaaca
     2521 gcggcgagcg cgccggatcg gtgagggcga gcagcaggat cgtgccgatc agcagcaact
     2581 gggagaacag cagccagccg cggcggcggc cgaatgctcg agtaaagaac ggcacatgca
     2641 gcgcatcgac cagcggcgcc cacaggaatt tgagcgtgta gggcgtgcca accaaggcca
     2701 atagtccgat ggtaccgagg tcgacgccgg attcgcgcat ccacacctgc agcgtcgaac
     2761 ccgacagcgc cagcggcagg ccggacgaaa agccgagcag caagacgatc agcacccgcc
     2821 gttgcagata caccgcgagc ccctcgcgcc aggaggcgcg gggctttgcg gattctttcg
     2881 gtgaactcga gggagcttcg ggtgtgttca tgaaaaggtg ttagcagatt cgcgctttgt
     2941 ccagcggatg gatgggtctc ttcccctctc cccttgtggg gccccttgtg ggagagggtg
     3001 gctcgcatcg ccagatgcga gacgggtgag gggtcacggt ctatcgatag aacgaaaccc
     3061 ctcatccggc gcggacttcg tccacgccac cttctcccac aaggggagaa gggaaggaag
     3121 agccgggcga tgacgcaggt gtgtcgcctc tcgcgcttca ctgcgcgaga aaagctactc
     3181 ccccgcctgc agctggcgcg gcagcggaaa cagctcgggc gcggctgatt tgacgaggcg
     3241 cggggtttcc gggggcgctt cgctcttgct gaaatcgagt tcctcgatcc gcccggcgcg
     3301 tttttcgatc ttgtcggcgg aaatcaggat ttgccggaca tcctcgttgg cctgtccgaa
     3361 atggttctgc agcttcagca cgcggtcgcg cagccggttg agatcgtcgc cgaggttgag
     3421 cacttcggtg cggatcaggt cggcggcgtc gcgcatccgc gcgtctttca ggatctgctg
     3481 catcacctgg atcgccagca tcaacagcga cggcgacacc aacaccaccc gggcgcggta
     3541 ggccttctgg atcacgtcgt caaagccgtc atggatctca gcgtagaccg attcggacgg
     3601 cacgaacatc agcgcggtat cctgggtttc gcctgcgatc aaatattttt cggcgatatc
     3661 gctgacgtgc ttcagcacgt cggcgcgcag ccgttgggtg gcgaatttct tttcctcgtc
     3721 ggtgcgcgcg tcatgcagcg cggtcatcgc ttcaagcgga aatttcgcgt cgatgcagag
     3781 cgggcgctga tcgggcagaa acaccacgca atccggccgc ttgccggtgg agagcgtgaa
     3841 ttggaattcg taactgcctt tcggcatgcc gtcttggacg atggcttcca tccgcgcctg
     3901 gccgaacgcg ccgcgcgatt gcttgttggc cagcacgtcg cgcagcgtcg tcacctggga
     3961 ggtcaggtcg gtgaggtttt tatgggcatt gtcgatgatg ccgagccgct cgtgcagcac
     4021 gcgcagactg tccatggtgt tgcgggtggt ctgttccatc gattggccga cgcggtgggt
     4081 caccgaatcc agccgctcgt tgacggcacg cgccatgtcg gcctggcggc cggccagttg
     4141 ctgccccatc gcatcgacgc ggccggtggc ctcgctctgg gcgcggagca tttcgctcag
     4201 gcgttcttca agttcgtcgg cgcgtatcgc ctgcgccatc gccaattccg cgccgcgccg
     4261 gccggaccgc gctatcacga tggcaatgct cagcagcaat accagcgcca gcgcgccgaa
     4321 accgatcagc gcgctgccgg tgcgtaccgg caggccgccc atcacaaaaa gaatctcgtt
     4381 catgagagcc cttgtagccg attcggacgc cggtgcgaac gaagagggaa catttatagg
     4441 taaatgatgc actaattttc gtggttaacg ccggcttaag attcgtggtt aacagcgcgt
     4501 taacagcaag cggcgcggat tgaccccggc aaatcagcgg cttaaatcgc ccgccatggc
     4561 cattcgtgaa atcatcatcc tgcccgacaa acagttgcgg ctcatctcca aaccgatcga
     4621 aaaagtcacg gcggaggttc gcaagctcgc cgacgacatg ttcgagacca tgtacgccgc
     4681 acccgggatc gggcttgccg cgatccaggt cgcgcagccg ctgcggctga taaccatgga
     4741 cctcgccaag cgggacgaag acggcgaaag caagccgcgg ccccgcgtct tcatcaatcc
     4801 ggaaattctg tcgtcgtcgg aagaactgtc ggtgtacgag gaaggctgcc tgtcgattcc
     4861 ggagtattat gaggaggtgg agcggccggc gcgggtgcgc gtacgtttca ccgatctcga
     4921 cggcaaggtg cacgaggaag aagccgaagg cctgttcgcc acctgcatcc agcacgaaat
     4981 cgatcatctc aatggcgtgc tgttcgtgga ttatctgtcg aaactgaagc gcgaccgcgt
     5041 gctgaagaaa tttgccaaag ccgccaagcg cgcggcggag tagggcgtga gccgctcagc
     5101 cgtcatgccc ggccttgtgc cgggcatccg cgtctttctc gccgataccc tagacgtgga
     5161 tggccgggac aagcccggcc atgacgagta aactgaacct tcattgggag atttccagct
     5221 tcatgccgct tcgcttgatc ttcatgggca cgccggattt cgcggtgccg acgctgctgg
     5281 agcttgtggc ccatggccac gagatcgcgg cggtctatac ccgcgcggca aaacccgccg
     5341 ggcgcggcat gaagttgcag ccgacgccgg tggaagtcca ggcgaggcgg ctcggcattc
     5401 cggtggccac accggcgacg ctgaagacgc ccgaagcgct gaaagcgttt agcgcgcatc
     5461 atgcggaagc agccgttgtc gtcgcctttg gcatgatcct gccgcaagcc attctcgata
     5521 ccccaaagct cggctgcttc aacctgcatg cctcgctgct gccgcgctgg cgcggcgcag
     5581 ccccgatcaa ccgcgccatc atggccggcg atcccgagac cggggtgatg gtgatgaaga
     5641 tggctgccgg cctcgacacc ggcgacgtgg cgatggccga acgcatggcg attaccgatg
     5701 cgatgacggc tgcggacttg catgacgcgc tggccccgct cggcgccgat ttgatggtgc
     5761 gcgcgatggg cgcgctcgaa cgcggcaggc tgcaactcac caaacaaagc gaagacggcg
     5821 tgacctacgc cgccaagatc gagaaggccg aggcgcggat cgcctggaac aagccggccc
     5881 gcgccgtgct gaggcatatt cacgggttgt cgccgtttcc cggcgcgtgg tgcgaggtgg
     5941 caagcgaaag cgagcaggtg cgcgtcaaga tcctgcgctg tgagatgatc gatcggtccg
     6001 gtgcaccggg cgacgtgctc gacgaccacc tcacgatcgc ctgccagcaa ggcgcattgc
     6061 ggattctcga actgcagcgc gccggcaagg cgccgatgaa ggccgcggac tttttgcgcg
     6121 gcacgccgct gaaggccggc gcgcggttcg cgtaacattg ttaaacgccg ccaaaccgtc
     6181 atacgcgggc ttgacccgcg tatccatctg tcttcaaaag agtctgacga agatgctgga
     6241 tggtcgggag cgcagacaag tttacgtagt ctgcgcaagg cagactatta tgcccggcca
     6301 tgacgaccga gacagatcgc aacccagatg ccccgctaca aactcaccat cgaatatgac
     6361 ggcgcgccgt tctgcggctg gcagatccag gacaatggcg cctcggtgca gggcgcgctg
     6421 gaaaccgcga tcaccgcgat ctgcggcgag caggttcgcg ttcacggcgc gggccgcacc
     6481 gacgccggcg tgcacgcact ggcgcaggtc gcgcattgcg atattgccaa acattttgtg
     6541 ccgggccggt tccgcgacgg cttgaatgcg catctgcggc cgcatccgat cggcgtgctc
     6601 agtgcggaga tcgtgccgga tacattcgag gcgcgattct cggcgaaaaa gcgtcattat
     6661 ctctatcgca tcagcaatcg ccgcgccaat ctcgcgctcg agatcggcca tgtctggcgg
     6721 ctgccgaggc cgctcgatac cgatgcgatg caggccgcgg cacagcgcct gatcggcaag
     6781 cacgatttta cgacttttcg cgataccgaa tgccaggcga aatcgccgga gaaaaccctc
     6841 gaccagctcg acgtcgtcag gctgggcgac gccgtcaaca tcgtgacctc ggcgcgttcg
     6901 ttcctgcaca gccaggtgcg ctcgatggtc ggctcgctgg tgtgggtcgg cgagggtcgc
     6961 tggaacgccg acgaccttgc ggccgccttg gccgcgcgaa accgcgccgc ctgcggcccg
     7021 gtcgccccgc ccgacgggct ctatctggtg cgggtggagt attagtttag actaccacta
     7081 tgaagccgtc atggcccggc ttgtcctcac ggttgtctgg cacggtccat gcttgtcatg
     7141 ccgcgttctc cgtggcgagg tcagcgcgaa tatagctgcg ccgtcgcgcg gcgcgggttg
     7201 catgtggtcc gaagtcaaat ctggactgtt gtggctcggg gggtggcg
//
LOCUS       DATNOI010000018         2611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1774254,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000018 DATNOI010000000
VERSION     DATNOI010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774254"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VN968_00640"
     CDS             complement(<1..375)
                     /locus_tag="VN968_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXL27790.1"
                     /translation="MSDVIDRPVPGQEASAKSRLRIIDCDVHPSLHARSDLNPFLAKR
                     WQEHLKTYGDHLRTPYIGTTPYPRSSPLIARRDAWPPTGGPPGSDLAFMRKQHLDPLD
                     IEFGILQVLDMFIFSQQNLEFGA"
     gene            complement(409..1470)
                     /locus_tag="VN968_00645"
     CDS             complement(409..1470)
                     /locus_tag="VN968_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL27791.1"
                     /translation="MSATRIDCDIHPAVGGTRTTLLPYLDDHWKEQVVSRAIDGLDLT
                     SYPPNMPLSGRADWRPANGKPGSDLAMVQRRAFDQLGASHAICNVLYGAQAVYDPYMA
                     SAFCRAINDWIATEWLARDSRLRASIVVPMQAPDLAIEEIERRAGDNRFVSILVLAQG
                     ESLLGRRHYWPVYQAAEKHRLPIAIHAGSQYRGAPSSIGWPSYRYEYYLAEAQAFQAQ
                     VLSLIYEGVFVKFPGLKVVLMESGVSWLPAFMWRANKTWRGVRVEVPWIDREPAAIMR
                     DHIRLTTQPFDSPPDAKGVADIIDMIGSDKMFLFASDYPHWQFDGDDAIPPHLPANIV
                     SRMCADNPLETFPRLSLTA"
     gene            complement(1944..2603)
                     /locus_tag="VN968_00650"
     CDS             complement(1944..2603)
                     /locus_tag="VN968_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27792.1"
                     /translation="MQDNAKRSQVQVRKPRVATAVAADPAAPATSPKPVKKPQLATAV
                     DATDSATPETSAKPAKKPQSARGPYYVDFRARTAASYGHAFVWYGKTSEKAVEVAGLH
                     PAGDEVPYILGHLMWVPSETGASYGDLDPEYLTANYRVYLNEADAKKVFAYIKDLQAT
                     SPLWNAQTTNCTAFIGRIASFMGLKVPFHLQRPENYINELKAMNGGRQMVQLSPDTNV
                     Q"
BASE COUNT          490 a          786 c          825 g          510 t
ORIGIN      
        1 ggcgccgaac tcgaggttct gctgcgagaa gatgaacatg tcgagcacct gcaggatgcc
       61 gaactcgatg tcgagcggat cgaggtgctg cttgcgcatg aaggcgagat cggatccggg
      121 cgggccgccg gtcggcggcc acgcatcgcg gcgcgcgatc agcggcgagg agcgcggata
      181 gggcgtggtg ccgatatagg gcgtccgcag atgatcgccg taggtcttca agtgctcctg
      241 ccagcgtttg gccaggaacg gattgaggtc gcttctcgca tgcaggctcg gatgcacgtc
      301 gcagtcgatg atccgcaagc ggctcttcgc ggaggcttcc tgtccaggca cggggcggtc
      361 gatgacgtcg ctcatgcgat cctccatctt aaggtctatg cggcaagtct atgcggtgag
      421 gcttagccgc ggaaaggttt caagcggatt gtcggcacac atgcgcgata cgatattggc
      481 cggcagatgc ggcggtatcg cgtcgtcgcc gtcgaattgc cagtgcggat aatcggacgc
      541 gaacaggaac atcttgtccg agccaatcat atcgatgatg tcggcaacgc ctttcgcatc
      601 gggcggactg tcgaacggct gcgtggtcag gcgaatgtgg tcgcgcatga tcgcggccgg
      661 ttcgcggtcg atccagggca cctcgacacg cacgccgcgc caggtcttgt tggcgcgcca
      721 catgaaggcc ggcagccagc tgacaccgga ttccatcagc acgaccttga ggccgggaaa
      781 tttgacgaac acgccctcgt agatcaggct cagcacctgg gcctgaaacg cctgcgcctc
      841 ggcgagataa tattcgtatc gataggacgg ccagccgatc gagctcggtg ctccccgata
      901 ctggctgccg gcatggatcg caatcggcag tctgtgtttc tcggccgcct gatagaccgg
      961 ccaatagtgc cgacgcccca gcaggctttc gccttgcgcc agcaccaata tggacacgaa
     1021 cctgttgtcg ccggcgcggc gttcgatctc ttcgatggca agatccggcg cctgcatcgg
     1081 caccacgatc gaagcccgca ggcgggaatc gcgcgccagc cattcggtgg cgatccagtc
     1141 gttgatcgcc ctgcagaatg cggaggccat atagggatcg tacacggctt gcgcgccgta
     1201 gagcacgttg cagatcgcgt ggctggcgcc gagctggtcg aacgccctgc gctggaccat
     1261 cgcgagatcg ctgccgggct tgccgttggc cggccgccag tcggcccggc cggacagcgg
     1321 catgttaggc ggatagctcg tgagatcgag gccatcgatc gcccggctca ccacctgttc
     1381 tttccagtga tcgtcgagat aaggcaacag cgtggtacgc gttccgccga cggcgggatg
     1441 gatatcgcag tcgatccgcg tggccgacat gacgtttcct gaaatcgtta tttgcggcca
     1501 gattatgccg catttccaat gtgccgccgg ccgtcccgca gcgcaagggt taagcggcgg
     1561 acgcccccgc ttcgcgtcga cgcctgtgac ccggttccct gcaaaacccg ctcatgcaag
     1621 cgattcggcg gcccgcgtca ggtcgggagc gagatagctc cgaaacagag gcgcattgac
     1681 cagcgcgcga aaacttggat gccgcgcgac accgccatcc gcgaccagca gaaattgctc
     1741 cgcaatctcg gcgaggattt ccagatgcct ggtcgcggtc ggggccggac gaaggcgtca
     1801 ttatcagaaa gcgctgattg aagtggccgc gcgccgaccc gttggcaacg atgcagctca
     1861 cgcgggaaag acgttcccgg ggccgccgat gaagttatgg ccgctcgaac aagaaaaagc
     1921 ggagccggtg cagctaaccg agcctactga acattcgtat ccgggctgag ctgcaccatt
     1981 tggcggccgc cattcatcgc cttaagctcg tttatataat tctcgggcct ctgcagatgg
     2041 aatggcacct tcaagcccat gaagctcgct atgcggccga taaaagccgt gcagttggtt
     2101 gtttgggcgt tccatagcgg agaggtcgcc tggagatcct tgatataggc gaaaaccttc
     2161 tttgcatccg cctcgttcaa atacacccgg taattcgcag ttaaatattc agggtccaaa
     2221 tcaccgtagc tcgcccccgt ttccgatggc acccacatca aatgtcccaa tatgtagggc
     2281 acttcgtcgc ccgccggatg cagacccgca acttcgaccg ctttttcgct tgttttgccg
     2341 taccaaacga aggcgtgacc atagctcgcc gcggtccggg ccctgaagtc cacgtaatag
     2401 ggccccctgg cagattgggg ctttttggcc ggtttcgcgc tggtctcagg tgtcgcggag
     2461 tcggttgcgt caacagccgt tgcaagctgg ggctttttga ctggtttcgg gctggtcgcg
     2521 ggcgcagcag gatcggcggc aaccgccgtt gcaacacgag gcttccgcac ctggacctgg
     2581 ctgcgcttgg cgttgtcttg caaaccttgc a
//
LOCUS       DATNOI010000019        11504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1953600,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000019 DATNOI010000000
VERSION     DATNOI010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11504
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953600"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VN968_00655"
     CDS             complement(<1..281)
                     /locus_tag="VN968_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HXL27793.1"
                     /translation="MAEKPKKPQKLRARLPRGLADRGPAEIAATRAMVEKIREVFERY
                     GFEPVETPAMEFTDALGKFLPDQDRPNEGVFSFQDDDEQWISLRYDLTAP"
     gene            complement(519..1409)
                     /locus_tag="VN968_00660"
     CDS             complement(519..1409)
                     /locus_tag="VN968_00660"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HXL27794.1"
                     /translation="MGISFDKIDGVIWYDGKLVPWGDANVHVLTHGLHYASCVFEGER
                     AYGGTIFKSTEHSERLKNSARILDFEIPWSVAEIDAAKELVLEKNNLPEAYLRPVAWR
                     GSEMMGVSAQTNTIHLAIACWTWPSYFDPAERLKGIRLGLAEYRRPDPATIPALAKAA
                     GLYMICTISKHKAEREGYADAMMLDWQGRVAECTGANIFFIKDGKIHTPIADCFLAGI
                     TRATAIDLAKRRGIEVIERRIMPEELSGFSECFITGTAAEITPVSEIARWKFKPGTIS
                     EQLMAGYTAEVQPKGKAAAA"
     gene            1720..2280
                     /locus_tag="VN968_00665"
     CDS             1720..2280
                     /locus_tag="VN968_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXL27795.1"
                     /translation="MADVNFSNPSSGPDSRATEAPSPVPRPGDLRWDIIELLFFAYRD
                     FVGDADHELEVFGFGRAHHRVMHFVHRYPGLKVADLLDVLRITKQSLGRVLKQLLDEG
                     YIVQKTGNNDRRQRLLFATAKGEALVGKLAGLQTDRINRALRDIGQPGAEIVRQFLRA
                     MIDHDDPDKVLETILGPANATAKAKE"
     gene            2285..3007
                     /locus_tag="VN968_00670"
     CDS             2285..3007
                     /locus_tag="VN968_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL27796.1"
                     /translation="MAQAATLARKPPQPADDAPHVLLVDDDRRIRDLLSRFLCGEGYR
                     VTTATGAQDARAKLLGLHFDLLILDVMMPGETGFELARFIRTSSSVPIIMLTARHEAE
                     SRIEGLQIGADDYVAKPFEPRELVLRIGNILKRAAPPPVETLEQAAFGPYLYHLDRGE
                     LRQGDEVIHLTDREREMLRILAIARGETVPRSALTGDGTVNERAVDVQINRLRRKIER
                     DPANPLFLQAVRGIGYRLVASP"
     gene            3018..4406
                     /locus_tag="VN968_00675"
     CDS             3018..4406
                     /locus_tag="VN968_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL27797.1"
                     /translation="MSTLDTGLTLIRTAAGRVAAANDWIGNAFKGWMPSGLYARALLI
                     MIVPMVILQSVVAFVFMERHWNTVTRHLSAAVVQDIASLIDVYKNYPQDKDRTLLQRI
                     ARQRLQLVVDFLPLGDMPPPGPKPFFSLLDQTLPVQLSRQIGKPFWIDTVGRSNLVEI
                     RIQLDDSVMRVFAQRSAAYVENSQIFIFWMLGTSSILLLVAVLFLRNQIKPILRLADA
                     AESFGKGREAPNFRPRGASEVRRASQAFLEMKARIERSIEQRTAMLAGVSHDLRTILT
                     RFKLELALIGDSPEVDGMRKDVDEMAGMLEDYLAFARGDSGEHAQPTDMTRALEELRS
                     DAERNGHAATVAFRGLPVVTVKPASFKRCLANLVSNAARHADAISITGHRDHRYLTVT
                     IDDDGPGIPPKMREEVFKPFRRLDDARNQDEGGTGLGLAIARDIARSHGGDITLGDSP
                     MGGLRAAVRIPV"
     gene            complement(4485..5024)
                     /locus_tag="VN968_00680"
     CDS             complement(4485..5024)
                     /locus_tag="VN968_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HXL27798.1"
                     /translation="MTRLFVSICLAVCLLTLLPALDARAVEMPIRKAGLWEMKVVRTG
                     SPTPEMTMQHCTDESTDKEMSSTFSPMAKEMCSKQDIQKTATGYVSDSICNVGAMSIT
                     SHSDIVGDFNSAYTVKSTSHHEGGSASVPRDATTTVEAKWLGACKPDQKPGDIVMPGG
                     LKMNIKDMEKLKALMPKPK"
     gene            complement(5072..5377)
                     /locus_tag="VN968_00685"
     CDS             complement(5072..5377)
                     /locus_tag="VN968_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27799.1"
                     /translation="MRIMGAVGLVAIWGALASVPAWANPDLINTVPSFKGNDTGGIIA
                     YPLATQIDVRQLAVDHCGTYGKVAKFLAVDPQYGGYISFACRWVPYGAAERPLRTKY"
     gene            5600..6088
                     /locus_tag="VN968_00690"
     CDS             5600..6088
                     /locus_tag="VN968_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27800.1"
                     /translation="MRDLTTTSLITTTSLKRLGFGAALGVLSLLVCGQTAQAANPMEL
                     NFGLFGPRYDGRVAGCEAALSTITSQFEEKESKFWNSSLQITAYGQVHETAFRPWQSD
                     NIPRRYCSADAMLSDGKMRTVHYSIIEDGGFAGFGQGVEWCVTGLDRNWAYNPRCKAA
                     RP"
     gene            6196..6924
                     /locus_tag="VN968_00695"
     CDS             6196..6924
                     /locus_tag="VN968_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2"
                     /protein_id="HXL27801.1"
                     /translation="MFCFLLLFRFLFLGIGRFLNFLGLLALAALIGVATAGTASAQDR
                     RQNAPGEFDFYVLSLSWSPSFCEAASERGNEGRAQAQCGGRPFSFVVHGLWPQYEHGF
                     PDYCQRPSPRLDRNIMASMLDLMPAPGLIFSEWDKHGTCSGLGARAYFETIRKARSAV
                     KIPAEYLELSAPKTVAPAEIEDAFIKVNPGLSAAAIAVTCDKRRLSEVRICMSKDLQF
                     RACDEIDRRACRSEQVEMPPMRGG"
     gene            7074..7934
                     /gene="rlmJ"
                     /locus_tag="VN968_00700"
     CDS             7074..7934
                     /gene="rlmJ"
                     /locus_tag="VN968_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HXL27802.1"
                     /translation="MNYRHAFHAGGFADVVKHIVLVRILTYLQDKQAAFRVIDTHAGA
                     GIYDLAGDQARRGGEWLTGIARVMQARFSETTLALVKPYLDIVRAFNPPAELKAYPGS
                     PLIARALLRPQDRLTACEVEPKARKRLIEALRRDIQARVVDLDGWMALPAFVPPNERR
                     GLVLIDPPYEAKDEFERIAAGFAEAFAKWPTGSYLLWYPVKSRRATDTLARHVAAVAG
                     AGAPPGKCLRLEFSVAPQAAGAGLNSAGLLIVNPPWTLAGELKAILPELEKPLGQGGA
                     SRFRLETPKP"
     gene            8243..8458
                     /locus_tag="VN968_00705"
     CDS             8243..8458
                     /locus_tag="VN968_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXL27803.1"
                     /translation="MAMTGTVKFFNGERGYGFIKPDDGGRDVFVHITAVERAGLKDLT
                     EGQRITFEVEPDKKGKGPKAVNLVISA"
     gene            complement(8827..9483)
                     /locus_tag="VN968_00710"
     CDS             complement(8827..9483)
                     /locus_tag="VN968_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HXL27804.1"
                     /translation="MSRLVLAAIALTGFAVSAQAADIPYYGPRPPAYTPPLTVAQPLD
                     CYSWAGPYLGGNLGYAWGSVDNNLTKPSGFAAGVQAGYNWQPSPSFVFGVEGDIQATG
                     ADETFAPWKFSNPWFGTVRGRAGYAFSNILVYGTAGLAFGELRGETFGLSESHANAGW
                     TAGVGAEFGFGQAGFSRGGWSAKVEYLYVDLANTNFTITGVPNGYRFGTLRAGINYHF
                     "
     gene            9774..>11504
                     /gene="uvrC"
                     /locus_tag="VN968_00715"
     CDS             9774..>11504
                     /gene="uvrC"
                     /locus_tag="VN968_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HXL27805.1"
                     /translation="MTSDSPDTPRAPRPPISQPGSRPDLPPDPSSGDIDPAMANAEEE
                     DEVLLPDVPEEANETVPEGPLAVGHAAIEHAVRHAPTSPGVYRMLNAVSDVLYVGKAK
                     NVRKRLSSYARVAAPQPARILRMIAATVTVEIVSTSTETEALLLEANLIKQLRPRFNV
                     QLRDDKSFPYILITGDHWAPQILKHRGAQSRPGQYFGPFASAGAVNRTITALQRAFLV
                     RSCTDGFFESRTRPCLLYQIRRCSGPCTGEIDFPDYTELVREAKDFLSGRSRLVKQEL
                     AGEMEKASAELAFETAALYRDRLAALSAIQSQQGINPRTVEEADVFAIHQEGGYSCVE
                     VFFFRTGQNWGNRAYFPRAEKSFTPEEVLASFLAQFYDDKPPPKLILLSHGIEECELL
                     ADALSIKAGFKVEVSTPRRGEKKELIAHALTNAREALGRRLADTATQGRLLAGLVTTL
                     GLPHPPKRIEVYDNSHIQGTNAVGAMIVAGPDGFIKNQYRKFNIKSEGLTPGDDYAMM
                     REVLQRRFKRLLTPPVEGDGAKPKADDDSFPQWPDLVIIDGGRGQLNAVREIFDSLGL
                     TQVALLAVAKG"
BASE COUNT         2073 a         3675 c         3589 g         2167 t
ORIGIN      
        1 ggcgcggtca ggtcgtagcg caggctgatc cactgttcgt cgtcgtcctg gaacgagaac
       61 acgccctcgt tgggacggtc ctgatccggc aaaaatttgc cgagcgcgtc ggtgaattcc
      121 atcgctgggg tttccaccgg ctcaaaaccg tagcgctcga acacttcgcg gattttctcg
      181 accatcgcgc gggtcgccgc gatctcggca ggcccgcgat cggccagccc gcgcggcagc
      241 cgggcacgga gtttctgggg ttttttcggt ttctcggcca tgggcggcgt tggtaccagc
      301 cgacgcgcga tgcggcaacc tcgcttgctt cttaacctcg ccccgctctt tgcggggaga
      361 ggtcgaaatt cgcgcgtagc gggattttcg ggtgaggggc agggcacgat gcctaccgca
      421 aattcagcat tcgcgggtcg gagagcccct cactccagcc ctctccccgc gaagggcggg
      481 gcgagggagc ggtcagcgcg tgccgcccgc aacccttact acgccgcggc ggccttgccc
      541 ttgggctgca cttctgcggt gtagccggcc atcagctgct cggaaattgt gccgggcttg
      601 aacttccaac gcgcgatctc cgataccggc gtgatctcgg cggcggtgcc ggtgatgaag
      661 cattcggaaa agcccgaaag ttcctccggc atgatgcggc gctcgatcac ctcgatgccg
      721 cggcgcttgg caaggtcgat cgcggtcgcc cgggtgatgc cggccaggaa gcagtcggcg
      781 atcggtgtgt gaatcttgcc gtccttgatg aagaagatgt tggcgccggt gcattcggcg
      841 acgcggccct gccagtccag catcatggcg tcggcgtaac cctcgcgctc cgccttgtgc
      901 ttggaaatcg tgcagatcat gtagaggccg gccgccttcg ccagcgccgg gatggtcgcg
      961 ggatccggcc ttcgatattc ggcaagacct aaccggatgc ccttcagccg ttcggcgggg
     1021 tcgaaatagc tcggccaggt ccagcaggcg atggcgaggt ggatggtatt ggtctgcgcc
     1081 gacacgccca tcatttccga accgcgccag gcgaccgggc ggagataggc ctcaggcaga
     1141 ttattctttt ccagcaccag ctctttggcg gcatcgatct cggcgaccga ccagggaatt
     1201 tcgaaatcca ggatgcgggc ggaattcttc agccgctccg aatgctcggt gctcttgaag
     1261 atcgtcccgc cataggcgcg ctcgccctcg aacacgcagc tcgcataatg caacccgtgg
     1321 gtcagcacat ggacgttggc atcgccccaa ggcaccagtt tgccgtcata ccagatgacg
     1381 ccatcgattt tatcgaacga aattcccatg ggctttctcc cggattgacg ctgatcgcgc
     1441 gcccgcgccg cggcgctgtt ggttacgcct gggccctcgc ctgcaccagc ctcgcagccg
     1501 gtttccatcc tcacatgtcc gtggacatgc ttttgtggaa ggcggaagtc cagtatggtt
     1561 gccgatatac cgctcgacaa tcgcacggta gataatttgc ggctctcgcc gccacctctg
     1621 ggccttctcc ggcccgtttg aaatgccact gccgactgaa cgacttaata tttcgttgcg
     1681 gccgatagtt tcgtaacatt gaacccaaga tatgtcaata tggctgacgt aaatttctcg
     1741 aacccctcgt ccggccctga ttcgcgagcc acggaggcgc catcccccgt cccgcggccc
     1801 ggcgatttac gctgggatat catcgagctg ttgttcttcg cctatcggga tttcgtcggt
     1861 gacgccgatc atgagctgga ggtcttcggg ttcgggcggg cgcatcaccg cgtcatgcat
     1921 ttcgtccatc gttatccggg cctgaaggtg gccgatctgc tggacgtact gcgcatcacc
     1981 aagcaatcgc tgggacgggt tctcaagcaa ttgctcgatg aagggtatat cgtgcagaag
     2041 accggcaaca acgaccggcg gcaacgtctt cttttcgcca ccgccaaggg agaggcgttg
     2101 gtgggcaagc tcgcaggcct gcagaccgat cgcatcaacc gcgcgctgcg ggacatcggt
     2161 cagccgggtg cggaaattgt ccggcagttc ctgcgtgcga tgatcgacca cgacgacccc
     2221 gacaaggtgc tggagacgat tcttggtccc gccaacgcaa ctgcaaaggc aaaggaataa
     2281 acccgtggcc caggctgcaa ccctcgcccg aaagccgccg caaccggccg acgacgcccc
     2341 gcatgtgctg ctggtcgacg acgatcgccg catccgcgat ctgttgtcgc gctttctctg
     2401 cggcgaaggt taccgtgtga ccaccgcgac cggcgcacaa gacgcccgcg ccaagctgct
     2461 cggcctgcat ttcgatctgt tgatcctcga cgtcatgatg cccggcgaaa ccggcttcga
     2521 actcgcccgc ttcatccgta cctcttcgtc ggtgccgatc atcatgctga cggcgcgcca
     2581 tgaggccgaa agccggatcg agggcctgca gatcggcgcc gacgattatg tcgcaaagcc
     2641 gttcgagccg cgcgaactgg tgctgcgcat cggcaatatt ctcaagcgcg ccgcgccgcc
     2701 gccggtggag acgctggagc aggccgcgtt cgggccctac ctctatcatc tcgatcgcgg
     2761 cgaactgcgc cagggcgacg aggtcattca tctcaccgat cgcgaacgcg agatgctgcg
     2821 cattctcgcc atcgcgcgcg gcgaaaccgt gccgcgcagc gcgctcaccg gcgacggcac
     2881 cgtcaacgag cgggcggtgg acgtgcagat caaccggctc aggcgcaaga tcgaacgcga
     2941 tcccgccaat cccctgttcc tgcaggcagt gcgcggcatc ggctatcgcc tggtcgcatc
     3001 gccttgagca gttgccgatg agcacgctcg acaccggcct cacgctgatc cgaaccgccg
     3061 ctggccgcgt cgcggcggcc aatgactgga taggcaacgc gttcaagggc tggatgccct
     3121 cgggactcta cgcccgcgcg ctgctgatca tgatcgtgcc gatggtcatc ctgcagtcgg
     3181 tggtggcgtt cgtgttcatg gagcggcact ggaacacggt gacgcggcat ttgtccgccg
     3241 ccgtcgtgca ggacatcgcc agcctgatcg acgtctacaa gaattatccg caggacaagg
     3301 accgcacgct gttacagcgg atcgcccggc agcggctgca gctggtggtg gatttcctcc
     3361 ccctcggcga catgcccccg cccggcccaa agccgttctt ctcgctgctc gaccagacac
     3421 ttccggtgca gcttagccgg cagatcggca agccgttctg gatcgatacc gtcggccgct
     3481 ccaatctggt ggaaatccgg atccagctcg acgattccgt gatgcgcgtc ttcgcccagc
     3541 gcagcgctgc ctatgtcgag aattcgcaga ttttcatctt ctggatgctc ggcacctcgt
     3601 cgatcctgct gctggtcgcc gtgctgttcc tgcgcaacca gatcaagccg atcctgcgcc
     3661 tcgccgatgc cgccgaaagt ttcggcaagg gccgcgaagc gccgaacttt cgcccccgcg
     3721 gggccagcga agtgcgccgt gcctcgcagg catttctcga gatgaaggca cgcatcgagc
     3781 gctccatcga acagcgcacc gccatgctgg ccggtgtgtc gcacgacctg cgcaccatcc
     3841 tgacccgctt caagctcgaa ctggcgttga tcggcgatag tccggaagtc gacggcatgc
     3901 gcaaggacgt cgacgagatg gccggcatgc tggaggacta tctcgcgttc gcgcgcggcg
     3961 acagcggcga gcacgcccag ccgaccgaca tgacgcgggc gctggaggaa ttgcgcagcg
     4021 acgccgagcg caacggccac gccgccaccg tggcgtttcg cggcctgccg gtggtgacgg
     4081 tgaagccggc gtcgttcaag cgctgccttg ccaatctggt ttccaacgcg gcacggcacg
     4141 cagacgcgat ttcgatcacc ggtcaccgcg atcaccgtta tctgacggtg acgatcgacg
     4201 acgacggacc cggcattccg cccaagatgc gcgaagaggt gttcaagccg ttccggcggc
     4261 tggatgacgc ccgcaaccaa gacgagggcg gcaccgggct cggccttgcc atcgcccgcg
     4321 acatcgcacg ctcgcatggc ggcgacatca cgctcggcga cagtccgatg ggcggacttc
     4381 gcgccgcggt gcggataccg gtgtaacgac ggatagagcg ttttcaagcg aagtggggat
     4441 cggttcgcgt caagaaaacg cgtccaatca agaatctacc gtcgttattt cggcttcggc
     4501 atcagggctt tcagcttttc catgtccttg atgttcatct tcaggccgcc gggcatcacg
     4561 atgtcgccgg gcttctggtc gggtttgcag gcccccagcc atttcgcctc gaccgtcgtc
     4621 gtcgcatcac gcggcacgct ggcggagccg ccctcgtgat gcgacgtgga cttgaccgtg
     4681 taggcggagt tgaaatcgcc gacgatatcg gaatgcgatg tgatcgacat ggcgccgaca
     4741 ttgcagatgg aatcgctgac gtaaccggtc gcggttttct ggatatcctg cttggaacac
     4801 atctccttcg ccatcggcga gaacgtcgag ctcatctcct tgtcggtgct ctcgtcggtg
     4861 caatgctgca tcgtcatttc cggcgtcgga gaaccggtcc gcaccacctt catttcccac
     4921 aggcccgcct tgcggatcgg catctcgacc gcgcgggcgt cgagcgcggg cagcagcgtc
     4981 agcagacaga ctgccaaaca aattgaaaca aacagccgcg tcatgctccg cgctcccggt
     5041 tactcagttc ggatagatgg gggtggccgg ctcagtactt ggtgcgcagc ggccgttcgg
     5101 cggcgccata cggcacccag cggcaggcga aggaaatgta gccgccatat tgcgggtcaa
     5161 ccgcaaggaa tttcgcgacc ttgccatagg tcccgcagtg atcgaccgcg agttgacgga
     5221 cgtcgatctg ggtcgccagc ggataggcga tgatgccgcc ggtgtcgttg cccttgaacg
     5281 aaggcacggt attgatcaaa tcaggattag cccacgccgg aacggacgcc agcgcccccc
     5341 atatggcaac caggcccaca gcccccatga ttcgcatcgc agactccatt tcgccccggc
     5401 aatatcttaa gcgcctgtcc cgccaaagtg gatacggtct tgcggtcaga acgcgtgtta
     5461 ctttagggcg taaccgtggc cattaaaaga accgctgaag ggccgacagt gacttatcgg
     5521 caggtgttgc cgcgttgcac ttgacctggc gtcggcttcg cggcaccgtc tgcgggtagg
     5581 atcatccggc ggattgccca tgcgcgacct caccaccaca tcgttgatca ccaccacttc
     5641 gttgaaaaga ctcggtttcg gcgccgcgct cggcgtcttg agcctgctgg tatgcggcca
     5701 gacggcgcag gcggccaatc cgatggagct gaatttcggc ctgttcggtc cgcgctatga
     5761 cggacgggtg gccgggtgcg aagcggcgct ctcgaccatc acctcgcagt tcgaggagaa
     5821 ggaaagcaag ttctggaatt cctcgctgca gatcaccgcc tatggccagg tccatgaaac
     5881 ggcgttccgg ccctggcagt cggacaatat tccgcgccgc tactgcagcg ccgacgccat
     5941 gctgagcgac ggcaagatgc gcaccgtgca ctattccatc atcgaggacg gcggttttgc
     6001 cggcttcggc cagggcgtcg aatggtgcgt gacggggctc gatcgcaact gggcctataa
     6061 tccacgctgc aaggccgccc ggccctgatt ttccgagttc gggtttttcg tgccggcggg
     6121 ttcaccggta ttttgttctt gaaatgttct catcgcctgc taggctgccc agcggtcagt
     6181 ctgggaggcg tcgccatgtt ttgttttcta ttgctttttc gttttctatt cctcggcatc
     6241 gggcggtttt tgaattttct gggcctgctc gcgctggcgg cgctgatcgg cgttgcgacc
     6301 gccgggacgg cgtcggcgca ggatcgccgg cagaacgccc ccggcgagtt cgatttttac
     6361 gtgctgtcgc tgtcatggtc gccctccttc tgcgaggcgg catccgagcg cggcaacgaa
     6421 gggcgcgcgc aggcccaatg cggcgggcgg ccgttttcct tcgtggtgca cgggttatgg
     6481 ccgcaatacg agcacggctt tcccgattat tgccagcggc cgtcgccccg gctcgatcgc
     6541 aatatcatgg cgtcgatgct ggatctgatg ccggcgcccg ggctgatctt ttccgaatgg
     6601 gacaaacatg gcacctgttc gggactggga gcgcgggcct atttcgagac catccgcaag
     6661 gcgcgctcgg ccgtgaaaat acccgcagaa tatctcgaat tgtcggcgcc caagaccgtt
     6721 gcgccggccg agatcgagga cgccttcatc aaggtcaatc ccggtctaag cgccgccgcg
     6781 atcgcggtga cctgcgacaa gcggcggctc agcgaagtcc ggatctgcat gagcaaggat
     6841 ctgcagttcc gcgcctgcga cgaaatcgat cgccgcgcct gccgcagcga gcaggtggag
     6901 atgccgccga tgcggggcgg gtgatctccc ccgccgtcat gtccaggctt gtcccgggca
     6961 tccacgtctt ccttcgtctt gttcgccaaa gacgtggatg gtcgggacaa gcccggccat
     7021 gacgaataga agacacgcct gtcttcatcc aagcggggcc gtaggttttc ctaatgaact
     7081 accgacacgc ctttcatgcc ggcggctttg ccgacgtcgt caagcacatc gtgctggtgc
     7141 gcattctcac ctatctgcag gacaagcagg cggcgtttcg cgtcatcgac acccatgccg
     7201 gcgcgggaat ctacgatctc gccggcgatc aggcgcgccg cggcggcgaa tggctcaccg
     7261 gcatcgcacg ggtgatgcag gcacgctttt ccgagaccac cttggcgctg gttaagccct
     7321 atctcgacat cgtcagggcc ttcaatccgc cagccgaact caaggcctat cccggatcgc
     7381 cgctgatcgc gcgggcgctg ctgcgtccgc aggaccgcct caccgcctgc gaagtcgaac
     7441 ccaaagcgcg caagcgcctg atcgaggcgc tgcggcgcga catccaggcg cgggtggtcg
     7501 accttgacgg ctggatggcg ctgccggcct tcgtgccgcc gaacgagcga cgcggcctgg
     7561 tgctgatcga tccgccttac gaagcaaagg acgagttcga gcggatcgcc gcgggcttcg
     7621 cggaggcttt tgcgaaatgg ccgaccggca gctatttgct gtggtatccg gtgaaaagcc
     7681 ggcgcgccac cgacaccctg gcgcggcatg tcgccgcggt ggccggcgcc ggcgcgcctc
     7741 cgggaaaatg cctgcgcctg gaattcagcg tcgcgccgca ggccgccggc gccggcctga
     7801 actcggcggg tctcttgatc gtcaacccac cgtggacgct ggccggcgaa ctgaaagcaa
     7861 tcctgcccga actcgaaaag ccgctcggcc agggcggcgc cagccggttc aggctcgaga
     7921 cgcccaagcc ttaattgacc aagccttgat tgaccaagcc ttggttgacc cggaggccca
     7981 aacgcgccgg tcggtggacg ccgcgaaaag tgcgtaggca atttgcggaa aaccgtatta
     8041 tgatcaaggc cattaggact ggctttacgt tccgcttccg cgaatggtag cggcggagtg
     8101 acaaggcctc acatatttca aaggcggatc ggcgaggtcg cttgatccgc ccggatggcc
     8161 aagctttccg acagcggatg tccggtcggg ccggtacgcg gcgcgcgtag cggcgaagca
     8221 ctacgggata ggagttttcc cgatggccat gactgggacg gtcaagttct tcaacggaga
     8281 acgcggctat ggcttcatca aacccgatga cggcggccgc gatgtattcg ttcatatcac
     8341 cgcggtggaa cgagcggggt tgaaggatct gaccgaagga cagcgcatca cattcgaggt
     8401 cgaaccggac aagaagggca aaggccccaa ggcggtcaat ctggtgattt cagcttagag
     8461 cgcgtgtcgc gccaggtgtg gaatcccgca ttcttgcggc gacaagagcg tagcgctgct
     8521 ccgatgactg cgtcgttgcc gggcttggcc cggcaatcca tccgctttcg aaattgcttc
     8581 tccagaaaat agatggatac gcggggccgt ccaggccatg atccgctcag attgaatcgg
     8641 atcatgatct gatctctttg tttgagcatg aaaaccggtt tccacttttc cggatcatgc
     8701 tctggcgcga agacgcgcgt cgcgcctttg cccgcgcatg acacctcgtc gtagagccaa
     8761 aaaaatcccg gccgcgcggc ggccgggagg ttgtgacaga tattttcttg ttgcagtgat
     8821 tggaagttag aagtgatagt tgatgccggc gcgcagcgtt ccgaaccggt agccgttcgg
     8881 tacgccggtg atggtgaagt tggtgttcgc cagatcgacg tagagatatt cgaccttcgc
     8941 gctccagccg ccccgggaga agccggcctg gccgaatccg aattcggcgc cgacgcctgc
     9001 ggtccagccc gcgttggcat ggctttccga caggccgaat gtctcgccgc gcaattcgcc
     9061 gaacgccagg ccggcggtgc catagaccaa aatgttgctg aaggcatagc cggcccggcc
     9121 gcgcacggtg ccgaaccacg gattcgagaa cttccacggc gcaaatgtct cgtcagctcc
     9181 ggtagcctgg atgtcgcctt cgacgccgaa cacaaatgat ggactcggtt gccagttgta
     9241 gccggcctgg acgccggccg caaaacccga cggcttggtc agattgttat cgaccgagcc
     9301 ccaggcatag ccgaggttgc cgccgaggta ggggccggcc cagctatagc aatcgagcgg
     9361 ctgagcgacg gttaagggag gagtataggc aggagggcgc ggcccgtaat aaggaatgtc
     9421 ggccgcctgc gccgagactg cgaaacccgt caatgcgatc gcggccaaaa ctaacctgct
     9481 catgctgctc tcccacgcaa ctgccgcgac cgcccggtcg gcttgcgcgc ggaggatttg
     9541 cgcaaggcga accttcatgg ttacggaact ccactgtttt ctgataaagg tttatcgaga
     9601 gtcttaagtt aaagggttac taagacccgc cgccggtcac ttcgcattgt taacaatgtg
     9661 ttaccggcga tcgctcgcgg tcagcttgac cccggcccgt caggtgtggg cggccgattc
     9721 cgggccgggc ttgatgctgg cgatgcccgg tttcagcgca tagtttaggc cccatgacca
     9781 gcgattcccc cgacacccca agggcgcccc gcccgccgat ttcgcagccc ggctcccgcc
     9841 cggatttgcc gccggacccc tcctccgggg atatcgaccc cgccatggcc aacgccgagg
     9901 aggaggacga ggtgctgctg ccggatgtcc ccgaggaggc caacgagacg gtgccggaag
     9961 gtccgctggc ggtcggccac gccgcgatcg aacacgcggt gcgccatgcc ccgacctcgc
    10021 ccggcgtcta tcgcatgctc aatgccgtga gcgacgtgct ctatgtcggc aaggcgaaaa
    10081 acgtccgcaa gcggctctcg tcttacgcac gcgtcgcggc gcctcagccg gcgcgcattc
    10141 tgcgcatgat cgcagccacc gtgacggtcg agatcgtctc cacctcaacc gaaaccgaag
    10201 cgctgctgct cgaggccaat ctgatcaagc agttgcggcc gcgcttcaac gtgcagctgc
    10261 gcgatgacaa gtcgtttccc tacatcctga tcacgggcga tcactgggcg ccgcagatcc
    10321 tcaagcatcg cggcgcgcaa tcgcggcccg ggcaatattt cggaccattc gcctcggccg
    10381 gcgcggtcaa tcgcaccatc accgcgctgc agcgcgcctt tctggtgcgc tcctgcaccg
    10441 atggtttttt tgaaagccgc acgcggccgt gcctgctcta tcaaatccgg cgctgttcgg
    10501 gaccctgcac cggcgagatc gattttcccg actataccga actggtgcgc gaggccaagg
    10561 atttcctctc cggccgcagc cgcctggtga agcaggaact ggccggcgaa atggaaaagg
    10621 cctcggccga gctcgcgttc gagactgcgg cactttaccg cgaccgcctc gcggcgctgt
    10681 cggcgatcca gtcgcagcag ggcatcaatc cgcgcacggt ggaggaagcc gacgtgttcg
    10741 ccatccacca ggaaggcgga tattcctgcg tcgaggtgtt cttctttcgc accggacaga
    10801 actggggcaa ccgcgcctat tttccccgcg cggagaaatc attcaccccg gaagaggtgc
    10861 tggcctcgtt cctcgcgcaa ttctacgacg acaagccgcc gccgaaactg atcctgctgt
    10921 cgcacggaat cgaggagtgc gaattgctcg ccgatgcgct ttccatcaag gcgggcttca
    10981 aggtggaagt ctccacgccc cggcgcggcg aaaagaagga actgatcgcg cacgcgctga
    11041 ccaatgcgcg cgaggcgctc ggccgcagac tggccgatac cgcgacccag ggccggctgc
    11101 tggccgggct ggtcaccacg ctcgggctgc cgcatccgcc gaagcggatc gaggtctacg
    11161 acaacagcca catccagggc accaacgcgg tgggcgccat gatcgtggcg gggccggatg
    11221 gcttcatcaa gaaccagtac cggaagttca acatcaagtc cgaaggcctg acgccgggcg
    11281 acgattatgc gatgatgcgc gaggtgctgc agcggcgctt caagcggctg ctgacgccgc
    11341 ccgtggaagg cgatggcgcc aaacccaagg cggacgacga ttcgtttccg caatggcccg
    11401 acctcgtcat catcgacggc ggccgcggcc agctcaacgc cgtcagggag atctttgaca
    11461 gcctcggcct gacccaggtc gcgctgctgg ccgtggcaaa ggga
//
LOCUS       DATNOI010000020         2587 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1222155,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000020 DATNOI010000000
VERSION     DATNOI010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222155"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VN968_00720"
     CDS             <1..368
                     /locus_tag="VN968_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HXL27806.1"
                     /translation="GRVFGTGGIAAGIALGAWSSALALMGRGATTFGFSIDAVARRRL
                     PRIVAAALAMGGLLWLTATLVPALTAGMHSIAQAVLAGVLIVGGMAIYALLLALFGVL
                     GWREAVSAIRQTTAPDLRN"
     gene            474..1526
                     /gene="trpS"
                     /locus_tag="VN968_00725"
     CDS             474..1526
                     /gene="trpS"
                     /locus_tag="VN968_00725"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXL27807.1"
                     /translation="MAFVERVFSGVQPTGNLHLGNYLGAIVNFVKLQETHNCLYCVVD
                     MHALTQPVEVWGGPAELARNTREVTAAFIASGIDPKKHIVFNQSQVSGHAELAWIFNC
                     VARVGWLNRMTQFKEKAGKDRENASVGLYDYPVLMAADILLYRATHVPVGEDQKQHLE
                     LSRDIAQKFNNDFGDSIRGHGFSDGLLFPLPEPLITGPATRVMSLRDGTKKMSKSDAS
                     DNSRINLTDDADTIAQKIRKAKTDPEPLPSEQKGLETRPEADNLVGIYAALSGRSKSA
                     VLGEFGGGQFSNFKNSLVELCVAKLAPIAAEMKRLVADPGHVDSILVDGADRARAIAA
                     ETMSATRDIVGFVRKR"
     gene            1815..2309
                     /locus_tag="VN968_00730"
     CDS             1815..2309
                     /locus_tag="VN968_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXL27808.1"
                     /translation="MTTQRRSYETGHKPKCLVIVDDTAEWDRAVYYASRWAIRVGGGV
                     VMLRIIETEDQNQQWLGVADIMRAEAHEAANAALDRAAGRANGIAAITPERVIREGAP
                     TEQILDVIDKDVDISMLVLAANPGAEGPGPIIAMMAGAVGSFPIPVTIVPGDLSDEDI
                     DALS"
     gene            2427..>2587
                     /locus_tag="VN968_00735"
     CDS             2427..>2587
                     /locus_tag="VN968_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HXL27809.1"
                     /translation="MFIQTEATPNPATLKFIPGRAVLDSGTMEFVNREAAARSPLAER
                     LFGIPGVTGV"
BASE COUNT          465 a          825 c          828 g          469 t
ORIGIN      
        1 tcggccgagt gtttggcacg ggcggcatcg ccgccggtat cgcgctcggg gcctggagca
       61 gcgcgctggc gttgatgggg cgtggtgcta cgacattcgg tttttcaatc gatgcggtgg
      121 cgcgccggcg gctgccgcgc atcgtggcgg ccgcgctggc gatgggcggg ctgctgtggc
      181 tgacggccac gttggtgcca gcgcttaccg caggcatgca ttcaatcgcg caggccgttc
      241 tcgcaggtgt gctgattgtg ggtggaatgg cgatttacgc cctgcttctg gccctgtttg
      301 gcgtactcgg ttggcgcgaa gcggtgagcg cgatccgaca aaccacggcc cccgacttgc
      361 gcaactgacc ccctcatggc aatggacggc gcatagcgcc tgttggcaat ggacggcgca
      421 tggcgcctgt tggcattgga cggcgcgaaa caggcacgcg cgggaagctg acaatggcat
      481 tcgttgaacg ggttttttca ggcgtccagc cgacgggcaa tctgcatctc ggcaattacc
      541 tcggcgcgat cgtcaatttc gtcaagctgc aggagaccca taactgcctg tattgcgtgg
      601 tcgacatgca cgcgctgacc cagccggtgg aggtgtgggg cggccctgcc gaactcgcgc
      661 gcaacacccg cgaagtcacc gcggccttca tcgccagcgg catcgatccg aaaaagcaca
      721 ttgtcttcaa ccagagccag gtgtccggac atgcggaact ggcgtggatc ttcaattgcg
      781 tggcgcgggt cggctggctc aaccgcatga cgcagttcaa ggaaaaggcc ggcaaggacc
      841 gcgagaacgc ctcggtgggc ctctacgatt atccggtgct gatggcggcc gacattctgc
      901 tctatcgcgc gacccacgtg ccggtcggcg aggatcagaa gcagcacctc gagctctcgc
      961 gggacatcgc gcagaaattc aacaatgatt tcggcgactc gatccgcggc cacggcttca
     1021 gcgatggatt gctcttcccc ctgccggagc cgctgatcac gggtcccgcg acccgggtga
     1081 tgtcgctgcg cgacggcacc aagaagatgt cgaagtccga tgcgtcggac aattcgcgca
     1141 tcaatctcac cgacgatgcc gacaccatcg cgcagaagat ccgcaaggca aagaccgatc
     1201 cggagccgtt gccgtcggag caaaagggtc tcgagacccg ccccgaggcc gacaatctcg
     1261 tcggcatcta tgccgcgcta tcgggccgct ccaaatccgc ggtgctcggc gaattcggcg
     1321 gcgggcagtt ttcgaatttc aagaactcct tggtcgaact ctgcgtcgca aaactggcgc
     1381 cgatcgccgc cgaaatgaaa cggctggtcg ccgatcccgg ccatgtcgat tcgatcctgg
     1441 tcgacggcgc cgaccgtgcc cgtgccatcg ccgccgaaac catgagcgcg acgagggata
     1501 tcgtcggttt cgttcgcaag cgctagttcc gactttgtga gatcggtgag tcgcttgctg
     1561 cccacgcttc gagacgctcg cgtcgctcgc tcctcagcat gaggttgttg tgtttcagca
     1621 agctagacct catcctgagg aggccgcgaa gtggccgtct cgaaggatgg gctacaatac
     1681 cgatttgtga ttctaggtac tagttccatc gcttttgcca aaattaccgc agcgcacagc
     1741 ctggtgtcgc cgcttgtcga ggatggctgc gccgtggcag catgcggcct gcttggcgca
     1801 gcaccgggac atgcatgacc acccagcgac gaagctacga gaccggtcac aagccaaaat
     1861 gtctggttat cgtcgacgac accgcggaat gggaccgcgc cgtctactat gccagccgct
     1921 gggcgatccg ggtcggcggc ggcgtggtga tgctgcgaat catcgagacc gaggaccaga
     1981 accagcaatg gctcggggtc gccgacatca tgcgcgccga ggcccacgag gccgccaatg
     2041 ccgcgcttga tcgcgcggcc ggccgcgcca acgggatcgc cgcgatcacc cccgagcgcg
     2101 tgatccggga gggcgctccg accgagcaga tcctcgacgt gatcgacaag gacgtcgata
     2161 tctcgatgct ggtgctggcc gccaacccgg gggcggaggg ccccggcccg atcatcgcca
     2221 tgatggccgg cgcggtcggc tcgttcccga ttccggtcac catcgtgccc ggggatctga
     2281 gcgacgagga tatcgacgcg ctatcgtagg cccctgatgt acttgcggaa ctgcgggtcc
     2341 gcgcctcttg accgtgcgtt tgcggtcgcc atctgctaag gatcactcac gcgccggcct
     2401 tgaaccggcg acgccggaga aaatcaatgt tcatccagac cgaagccacg cccaatcctg
     2461 ccaccctgaa attcatcccc ggccgcgccg tactcgacag cggcaccatg gaattcgtca
     2521 accgcgaggc cgccgcgcgt tcgccgctcg ccgagagact gttcggcatc cccggtgtca
     2581 ccggcgt
//
LOCUS       DATNOI010000021         3121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2674446,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000021 DATNOI010000000
VERSION     DATNOI010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2674446"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..495)
                     /gene="bamE"
                     /locus_tag="VN968_00740"
                     /pseudo
     CDS             complement(15..495)
                     /gene="bamE"
                     /locus_tag="VN968_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510089.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
     gene            603..1154
                     /locus_tag="VN968_00745"
     CDS             603..1154
                     /locus_tag="VN968_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HXL27810.1"
                     /translation="MLWPFNHFRKPRVPSRGTIEGIYGMIVTQAREPLFYRDLGVPDT
                     VNGRFDLLVLHLWMVLRRLKTIEGGAELSQALFDGFCDDMDANLREMGVGDLTVPKRM
                     QAFGEAFYGRAAAYDQALTAGEEPLARALCKNILNGAELEKARQLAFYAGAAIAALTG
                     LDDATVQSGAWRFPSPARVGAQQ"
     gene            1151..1741
                     /locus_tag="VN968_00750"
     CDS             1151..1741
                     /locus_tag="VN968_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HXL27811.1"
                     /translation="MSRAGSMTNKADSWSVPITVAQIPDTGLHRDIEAGPAAREAMAE
                     VAGLRQILSASASLDVTPQSGGRVHVEGRVRARVGQTCVVTLDPIENDIDEPIDLIFA
                     PPEQIPQLADLVDEAAESDAEIPDPPEPIENGVIDLGRLATDALFLGIDPYPRRPDAV
                     FEPPVVVADPEDHPFAALKALRLDAKPTDAKKPRNG"
     gene            2063..>3121
                     /gene="plsX"
                     /locus_tag="VN968_00755"
     CDS             2063..>3121
                     /gene="plsX"
                     /locus_tag="VN968_00755"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HXL27812.1"
                     /translation="MPQKVRIALDAMGGDVGAAVVIPGAAISLTRHPDTEFLLFGERS
                     LIEVQLAKHPVMKAVSRVIHTDVAVGMNDKPSQALRRGRKTSSMWLAIDAVKKGDADV
                     AISAGNTGALMAMARFCLRMLPGIDRPAIAAVWPTIRGDSVVLDLGATIGGDAHHLMA
                     LAVMGGAMASVLFDLERPTVGLLNIGVEEIKGREEIREAAELLRAMDLPQLEYIGFVE
                     GDGIGKGAADVIVAEGFSGNIALKAAEGTARQINDFLRAALSRTWRSKIGYLFAKNAF
                     KALRDKLDPNKSNGGVFLGLNGIVVKSHGGTDAEGFAYAVDVGYEMVRYDLLTKINRM
                     LNRDGSPPPTAPTAQEAVS"
BASE COUNT          593 a          931 c         1010 g          587 t
ORIGIN      
        1 gaggcgaagc ctcgctagaa gctgagcagc ttgaacagcg gggtcaggta gctgagctcc
       61 tggcccgatg tcggcgtggt gcggctgatg aagtcgaata tcttgccgtc ctgtaggccg
      121 taattggcga gccgctgcac ctggcggttc ttgtcgaaat agatcgcgat gacccgctgg
      181 tcgacgacct tctggttcat gaacgcaacc atgcgctcgg agcgttgcga aatgtagtag
      241 aaaacttcgc cgttgagggt tgcgaccgtg gacggcgttc ccatcacgat cagcacctga
      301 tcctggctcg ccgatcggga tttgctccag cgcgcccgtc ggcaggatgt agcccttctg
      361 aaactgctcg ccggtgcagc cgccgagtac cgcgcacacc aaagcgacgg ccgcggccgc
      421 gcgcatgccg cgccagcgcg cagccaagcc gcgcgccgag cacacgcttg ggcgtgtctg
      481 gcttgtttca atcatgccgg aactggtccc gtccccttgc atcgcgcgag gcgttgacat
      541 accgggcagc acgttggatt gcaacatgcg cgggcccgga tggcccgctg gcggaacccg
      601 caatgctttg gccgttcaat cacttcagga aaccccgggt accgtcgcgc ggcaccatcg
      661 agggcatcta tggcatgatc gtgacgcaag cgcgagaacc gttgttttat cgggacttgg
      721 gcgtgccgga cacggttaat ggccgtttcg atctgctggt gctgcatttg tggatggtct
      781 tgcggcgctt gaagacgatt gagggtggcg cggaactttc ccaggcgttg ttcgatggtt
      841 tctgcgacga tatggacgcg aacctgcgcg aaatgggcgt cggcgatctc accgtcccga
      901 agcgaatgca ggcctttggg gaggccttct acggccgggc agcggcctat gatcaagcct
      961 tgaccgccgg cgaggaaccg ctggcgcggg cgttgtgcaa gaatatcctg aacggagcgg
     1021 aactcgaaaa agctcggcag ctcgcgttct atgctggggc ggccatcgcg gcgctgacag
     1081 gattggacga tgcgacggtg cagagcggcg cgtggaggtt tccttcgccg gcgcgagtgg
     1141 gcgcacagca atgagcagag cgggcagcat gacgaacaag gccgattcct ggagcgttcc
     1201 gatcaccgtg gcgcaaatac cggacaccgg attgcatcgc gatatcgagg ctggccccgc
     1261 cgcacgcgag gccatggctg aagtcgccgg cttgcgccaa attctgtcgg cgagtgcttc
     1321 gctggatgtg actccacaga gcggaggtcg cgtccatgtc gaagggcgcg ttcgggcgcg
     1381 ggtcgggcag acctgtgtcg ttacgctcga tccaatcgaa aacgacatcg acgagccgat
     1441 cgatctgatc ttcgctccgc ctgagcaaat tccacaacta gccgatcttg tcgatgaagc
     1501 cgccgaaagc gacgccgaga ttccagaccc gccggagccg atcgaaaacg gcgtcattga
     1561 tcttggccgg ctcgcaaccg acgcgttgtt cctggggatt gatccctatc cgcgcaggcc
     1621 ggacgcggtt ttcgagccgc cggttgtggt tgcagatccc gaggatcacc cgtttgccgc
     1681 actcaaggca ctgcggctcg acgcaaaacc gaccgacgcg aagaagccca ggaacggcta
     1741 aagaacggct aacgaaccgc cgaaggcggg ccgaagaacc gttgcgttcc cattccagca
     1801 aatatggatg cgagatcggt tcgatatttc gtactaagac actgacctat ctggatatat
     1861 ctgtgtgaat cggttccgcc ttaccaccgc ccggcattca aaaagagggc ggtcaagagg
     1921 ttgtgtcggc acagggacac gctattgtcg cggcccggcc ggggtgcagt gttgcgcttc
     1981 gccgatcgcc gccatccatg gcgatttcca gtccgcggcc gagcgcatca agaccgcaca
     2041 agatcaggtt tccgggacgt tcatgcctca aaaggttcga atcgcgcttg acgccatggg
     2101 tggcgatgtg ggcgcagcgg tggtcattcc cggcgccgca atttcgctca cccggcaccc
     2161 cgacaccgaa ttcctgctgt tcggcgaaag gtcattgatt gaagtgcaac tcgccaagca
     2221 tccggtgatg aaggcggtct cgcgcgtcat tcataccgat gtggccgtcg gcatgaacga
     2281 caagcccagt caggcgttgc gccgcggccg caagacctcg tcgatgtggc ttgccatcga
     2341 cgccgtcaag aagggcgatg ccgacgtcgc gatatccgcg ggcaacaccg gcgcgctgat
     2401 ggcgatggcg cgattttgtc tgcgcatgct gcccggcatc gaccgaccgg cgatcgcggc
     2461 ggtgtggccg acgatccgcg gtgattcggt cgtgctcgat ctcggcgcca ccatcggcgg
     2521 cgatgcgcat cacttgatgg cgctcgcggt catgggcggc gccatggcga gcgtgttgtt
     2581 cgatctcgag cggcccacgg tcggcctgct caacatcggt gtcgaggaaa tcaagggccg
     2641 cgaagaaatt cgggaagccg ccgaattgct gcgcgcgatg gatcttcctc agcttgaata
     2701 tatcggcttc gtcgaaggcg acggcatcgg caagggcgcc gccgacgtca tcgtcgctga
     2761 aggtttcagc ggcaatatcg ccttgaaggc ggccgagggg acggcgcgcc agatcaacga
     2821 ttttctccgc gcggcgctgt cgcgcacctg gcgctccaag atcggctatc tgttcgcaaa
     2881 aaatgccttc aaggcgttgc gcgacaagct ggatccgaac aagtcaaacg gcggggtgtt
     2941 cctcggcctg aacggcatcg tggtgaaaag ccatggcggc accgacgccg agggctttgc
     3001 ttacgcggtc gatgttggct atgagatggt ccgctacgat ctcctcacaa agatcaatag
     3061 aatgctcaac cgtgacggca gcccgcctcc cacggcgccg accgcgcagg aggctgtctc
     3121 g
//
LOCUS       DATNOI010000022         4056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2671701,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000022 DATNOI010000000
VERSION     DATNOI010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2671701"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VN968_00760"
     CDS             complement(<1..933)
                     /locus_tag="VN968_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HXL27813.1"
                     /translation="MGAPSSRRSESRGCGNIIETQEDVTPAVLAAMAQTPDDRLREIA
                     AAFIRHIHAFAREVKLTEDEYDVGIDFLNRIGQATSDRHNEGILFADAIGLSTLVCLI
                     NNGNAGATETASALLGPFWRMNSPKTENGGSIVRSPTPGPELLVSGKVLDAAGKPIEG
                     VEVDVWQSSPIGLYENQDDTQADMNLRGKFTTDAQGCFRFRSVKPAGYPVPTDGPVGD
                     MLRAQSRHPYRPAHIHFLGFKPGFKTLITQVFVDDDQHLGSNAVFGVTRALIGDYRRH
                     DSGAPPAPDMKAPWYSLDYTFVMEAGEAKLPVPPI"
     gene            1058..2098
                     /locus_tag="VN968_00765"
     CDS             1058..2098
                     /locus_tag="VN968_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXL27814.1"
                     /translation="MNRAGPTTLKQIAAATGVHTSTVSRALDPHKRHLVADDVAERIV
                     HLAQSLGYQPNRVATSLRTGRSHLIGVLLPDIANPVFAPILSGIAESLASEGYAPIVA
                     DAGNDSSRQIAFVDNLLNQRVDGLILATVSRDDDVVGHCLDRGMPVVLVNRFEFRDRV
                     SSIVSDDELGMQLAVDHLVSTGHRYIGHLSGPLNTSTGSLRRDGFSRAMAHHGLPVHV
                     EEASHFTREAGVAPARALIAGTAGITAIVAANDLLALGIFDAMRMLGLRCPDDISVVG
                     HNDMPLVDLISPPLTTVRIEHREMGRGAAKLLLEEIDHRGAAAQHVILQPELIVRAST
                     TALRMPSEIGNT"
     gene            2248..3771
                     /locus_tag="VN968_00770"
     CDS             2248..3771
                     /locus_tag="VN968_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL27815.1"
                     /translation="MADEQAPPAGPDLTLGVDLSEFTGETLLGHVGEEDVLLVRSGSD
                     IFAIDAHCSHYHGPLAEGLVVGESVRCPWHHACFNLRTGEAARAPALNPLAVWQVEQG
                     GNRIFVRQKRPQPKPGGKPPAESPGSILIVGGGAAGFAAAEMLRRQEYRGSIVMLSSD
                     AAPPVDRPNLSKDYLAGSAPEDWLPLRPDSFYTEAGIELRLNTDVTSIDTKARNVIIA
                     GGETISYDRLLLATGAEPVRLPIPGADQPHVHVLRTLADCRAIIESAKGARRAIVIGA
                     SFIGLEAAASLRARDIEVHVVGLEARPMERVLGPEMGDFIRALHEEHGVVFHLGDTVV
                     AIDGKRATLKSGRVLEAELVVVGVGVRPRLALAEKAGLALDRGIAVNAYLETSIAGIY
                     AAGDIARWPDPHSRENIRVEHWVVAERQGQAAARNMLGQREAFDAVPFFWSQHYDVPI
                     NYVGHAETWDEIAIDGDVAAKDCLLQYKRQGRVLAVASIYHDLASLKAELQMEQATP"
     gene            complement(3768..>4056)
                     /locus_tag="VN968_00775"
     CDS             complement(3768..>4056)
                     /locus_tag="VN968_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HXL27816.1"
                     /translation="AAIRQAKAKTGTAEELARRIKEGAIPIISDVEGFMAYYVVYAPD
                     DTVTAISLFNNFAGAEEANRRALAWIEQNLGPLLIGPASAVAGPVIVHTLA"
BASE COUNT          724 a         1266 c         1308 g          758 t
ORIGIN      
        1 gattgggggc acgggaagct tggcctcacc cgcttccatc acgaaggtgt agtcgagcga
       61 gtaccagggc gccttcatgt ccggcgccgg cggcgcgccc gaatcgtgcc gccggtaatc
      121 gccgatcagc gcgcgggtca cgccaaacac ggcgttactg ccgaggtgct ggtcgtcgtc
      181 gacgaacacc tgggtgatca gcgtcttgaa tccgggcttg aagccgagaa aatgaatatg
      241 cgcgggccga tagggatgcc gggactgggc gcgaagcatg tcgccgacgg gcccgtcggt
      301 cgggaccggg tatcccgccg gcttgacgga tcggaaacgg aaacagccct gcgcgtcggt
      361 ggtgaacttg ccgcgcaagt tcatgtcggc ctgggtatcg tcctggtttt cgtaaagccc
      421 gatcggcgag gattgccaga catcgacctc cacgccttcg atcggcttgc cggccgcgtc
      481 gagcaccttg cccgatacca acagctccgg cccgggcgtc ggagaacgaa cgatcgagcc
      541 gccgttttcg gtcttcggcg agttcattcg ccagaacgga ccaagcagcg ccgacgcggt
      601 ttcggtggcg ccggcgttgc cgttgttgat caggcatacc agcgtcgaga ggccgatggc
      661 gtcggcgaag agaatgcctt cgttatgtcg gtcgctggtc gcctggccga tgcggttcaa
      721 aaaatcgatg ccgacgtcgt attcgtcctc ggtcagcttc acctcgcggg cgaaggcatg
      781 gatatgccgg ataaaagccg ccgcgatctc gcgtagccgg tcgtccgggg tctgcgccat
      841 cgccgccaga accgcaggcg tcacatcctc ctgggtctcg atgatgttgc cgcaaccgcg
      901 gctctcgctg cggcgtgagg acggtgcacc catggacatc ttccggtttt gaggcatttg
      961 tctgcaagcg tttgcatcct atattgcaat cgattgcaag aagtcaagcc ctctgccata
     1021 gtgaagcgct acgacgaccc aactacaggg acacctcatg aatcgcgcag gacccacgac
     1081 gcttaaacag attgcggcag ccaccggcgt ccacacctca acggtgtccc gtgcgctcga
     1141 tccgcacaag cgccacctgg tggccgacga tgttgccgag cggatcgtgc atctggcgca
     1201 atcgctcggc tatcagccga accgcgtggc aaccagcctg cgtaccggac gatcgcacct
     1261 gatcggcgtc ttgttgccgg atatcgccaa tccggtgttc gcgccgatcc tcagcggcat
     1321 cgccgaatcg ctggcgagcg aaggttatgc gccgatcgtc gccgatgccg gcaacgactc
     1381 ctcccggcaa atcgcgtttg tcgacaacct tctcaaccag cgcgtcgacg ggctgatcct
     1441 ggcgacggtg tcgcgcgacg acgacgtggt cggacattgc ctcgaccgcg gcatgcctgt
     1501 ggtcctcgtc aaccgtttcg agtttcgcga ccgggtatcg tcgatcgtat cggacgacga
     1561 actgggcatg caacttgccg tcgatcatct ggtgtcgacc ggccatcgct atatcggcca
     1621 tttgtcggga ccgctcaaca cctcgacggg atcgttgcgg cgggatggat tttcgcgcgc
     1681 gatggcccat cacggcctgc cggttcacgt cgaagaagcc tcgcacttca cgcgtgaggc
     1741 cggcgtcgcg ccggcgcgcg cgctcattgc cggcactgcg gggattaccg cgatcgtggc
     1801 ggccaacgat ctcttagcgc tcggaatatt cgatgcgatg cggatgctcg gcctgcgctg
     1861 ccccgatgat atttcggtgg tcggccacaa cgacatgccg ctggtcgatc tgatctcgcc
     1921 gccgctgaca acggttcgaa ttgagcatcg cgaaatggga cgcggcgcgg cgaagttgct
     1981 gctggaagaa atcgatcacc gcggcgcggc cgcgcagcat gtcatcctgc aaccggagct
     2041 gattgtcagg gcatcgacga cggcgttgcg aatgccctcc gaaatcggaa acacttagct
     2101 tgacgccaaa tagggcgcgc cttctaatca acgtgaacgt ggaatgtcca atccagggac
     2161 ttttgccgcc gatcaattgg cgagaaaagg atccgttcgt ggttctcgcc gatcgagaag
     2221 ctgttcaaac tctcgaggga gcaaaacatg gctgatgaac aagccccacc tgccggcccg
     2281 gatctgaccc tgggcgtcga tctgtccgaa ttcaccggag agaccttgct cggccatgtc
     2341 ggcgaggaag acgttttgct ggtgcgctcg gggtcggaca tcttcgccat cgacgcgcat
     2401 tgtagccatt atcatgggcc cttggccgag ggattggtgg tcggcgagag tgttcgctgc
     2461 ccctggcatc acgcctgctt caatttgcgc accggagagg cggcccgcgc tccggctctt
     2521 aatccgctcg cggtctggca agtcgagcag ggcggcaacc gcatcttcgt gcggcaaaag
     2581 cgtccacagc cgaagccggg cggcaaaccg ccggccgagt cgcccggcag tatcctgatt
     2641 gtcggcggcg gcgccgcagg ctttgccgcg gcggagatgc tgcggcggca ggaatatcgc
     2701 ggcagcatcg tgatgctgag cagcgatgcc gcgccgccgg tggaccggcc gaacctgtca
     2761 aaggactacc ttgccggcag cgcgccggag gactggctgc cgctgcggcc cgacagtttt
     2821 tacaccgaag ccgggatcga gctgcgcctc aacaccgacg tcacttcgat cgacaccaag
     2881 gcacgcaatg tcattattgc cggcggcgag acgatttcct atgaccgcct gctgctggcg
     2941 acaggagcgg agccggtgcg cctgccgatt ccgggcgccg accagccgca tgtccacgtg
     3001 ctgcgcacgc ttgccgattg ccgggcgatc atcgaatcag caaaaggcgc gcgccgcgcc
     3061 attgtgatcg gcgccagctt catcgggttg gaagctgcag cgtcgttgcg cgcgcgcgac
     3121 atcgaggtcc atgtcgtcgg actcgaagcc cggccgatgg aacgcgtgct tggtccggaa
     3181 atgggcgact tcattcgcgc cctgcacgaa gagcacggcg tcgtcttcca tctcggcgat
     3241 acggtggtcg caatcgacgg caagcgcgcg acgttgaaga gtggccgggt gctggaggcc
     3301 gagcttgtgg tggtcggcgt cggggtgagg ccgcgcctcg cattggcgga gaaggccggt
     3361 cttgcgctcg atcgcggcat cgcggtgaat gcgtatctcg agaccagcat cgcggggatt
     3421 tacgccgccg gcgatatcgc gcgctggccc gatccgcatt cccgcgagaa tatccgggtc
     3481 gagcattggg tggtggccga acgccagggc caggccgccg cgcgcaatat gctggggcag
     3541 cgcgaggcat tcgacgccgt gccatttttc tggagccagc actacgacgt gccgatcaat
     3601 tatgtcggcc acgccgagac atgggacgag attgccatcg acggcgacgt cgcggcgaaa
     3661 gattgtctgc tgcaatacaa gcgccagggc cgcgttcttg ccgtggcctc gatctatcac
     3721 gatcttgcaa gcctcaaggc tgaacttcag atggagcagg cgacgcccta ggccagcgta
     3781 tgcacgatca ccgggccggc gaccgcggaa gccggtccaa tcagcagagg cccgagattt
     3841 tgctcgatcc aggcgagcgc ccgcctgttt gcttcctccg cgcctgcgaa attgttgaag
     3901 aggctgatcg ccgttacggt gtcgtcgggt gcatagacca cataataggc catgaagcct
     3961 tcgacatcgc tgatgatcgg aatcgcgccc tccttgatcc tgcgggccag ctcctcggcc
     4021 gtgccagtct ttgccttggc ctgacggatc gcggcg
//
LOCUS       DATNOI010000023         2206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1059441,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000023 DATNOI010000000
VERSION     DATNOI010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059441"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VN968_00780"
     CDS             <1..504
                     /locus_tag="VN968_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL27817.1"
                     /translation="LTRSGPNRHGRSHKNNKQTLGRNNMQFRLNFRPTALAAFGLLAA
                     AAAFSAGAHAADEWPARDITFYVPYAPGGSTDPISRKYAELLEKQLEVKVIVENKPGA
                     SATIGTGAVIRAAPDGYTIGLGSTSSLAYQPLEMKGLAWKSANDYQSIAKLSDLPAIL
                     AVPLAAT"
     gene            587..1075
                     /locus_tag="VN968_00785"
     CDS             587..1075
                     /locus_tag="VN968_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HXL27818.1"
                     /translation="MMRTPAAQAQTAPGIEQTFSGSLTAMPSEILGVSGAFYVPVYSS
                     VSISQGKVRADFSVTLSIHNASETRPLVLRRIAYFDTNGKMVENYLKAPVALKPFSTV
                     EVFVPTTDVRGGTGANFVVDWAAAGEIAEPVVEALMFGGLGSGHYAFISQGRPLKVVG
                     KN"
     gene            1137..>2206
                     /locus_tag="VN968_00790"
     CDS             1137..>2206
                     /locus_tag="VN968_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HXL27819.1"
                     /translation="MTSAQFDFAPLLPAGLPAPAAKWAGLAKYSFIGGNNDPDQVPID
                     GLIEAVDAVLKREGKTLATYGLASGPQGYRRLREFLTTKLKRDAGINCVADDILIVSG
                     SLQALDLVNATLLARGDTVIIEQDTYQGALNRLTRLGINVVGIPLDREGMRMEALAAA
                     LEDLKRRSITPKYIYTIPTVQNPTGTIMPEARRHELLKLAEQYAVPVFEDDCYADLIW
                     DGKRPPALYAMNTNGGVIHIGSFSKSIAPALRVGYIVAPWAALSRMLALKTDAGSGAL
                     EQMVLAEYCAPHFATHVPKLTRGLRAKLDTLMEALNAQFGTSAEFEDPKGGIFLWVKL
                     PDNVDTLKLYQSALAAGVAINPG"
BASE COUNT          435 a          715 c          668 g          388 t
ORIGIN      
        1 ttgacgagat ccggtcccaa tcgccatggt cgcagccata aaaacaacaa gcagacgctg
       61 ggaagaaaca acatgcagtt tcgactgaat ttccgtccga cggcattggc cgccttcgga
      121 ttgctggcgg cggccgcggc attttccgcg ggcgcacacg ccgccgacga gtggcccgcc
      181 cgcgacatca ccttctatgt accctacgcg cccggcggct ccaccgaccc gatcagccgc
      241 aaatatgccg agcttttgga aaagcaactc gaggtcaaag tgatcgtcga gaataagccc
      301 ggcgcttccg cgaccatcgg taccggagct gtgatccgcg ctgcgcccga cggctacacc
      361 atcggcctcg gctcgacctc gtcgctcgcc tatcagccgc tggaaatgaa ggggctggcc
      421 tggaaatccg ccaacgacta ccagtcgatc gcgaaacttt ccgatctgcc cgccatcctc
      481 gcggtgcccc tcgccgcaac ctaaaggcac gcacgtggag gtcaaacgag caatgtgtca
      541 catccggatg ggatgctatt atctggcgat cctggcttgc ctgctgatga tgcgcacgcc
      601 cgccgcacag gcccagaccg ctccggggat cgagcagacc ttctccggtt cgctcaccgc
      661 gatgccatcg gagattctcg gcgtgtccgg cgcattctat gttccggtct attcgagcgt
      721 ctcgataagc cagggaaagg tgcgggcgga tttctcggtg acgctgagca tccacaatgc
      781 ttcggagacg cgcccacttg tcttgcgccg gatcgcatat ttcgacacca acgggaaaat
      841 ggtggaaaac tacctcaagg cgccggtggc gctgaagccg ttctcgaccg ttgaagtctt
      901 cgtccccacc acggacgtcc ggggcggaac cggcgccaac ttcgtggtgg attgggccgc
      961 cgccggcgag atcgccgaac cggtggtcga agcgctgatg tttggtggac tcggtagcgg
     1021 ccattatgcg tttatcagcc agggccggcc gttgaaggtt gtcggcaaga actagcctgc
     1081 agggctgccg cgatagcgga cgctctcaac aagaaaataa aacggggatg acgtccatga
     1141 catcagcaca attcgatttt gcgcccttgc tgcccgcggg attgcccgcg cccgcggcga
     1201 agtgggcggg gctcgcaaag tacagtttta tcggcggcaa taacgatccg gaccaggttc
     1261 cgatcgacgg gctgatcgaa gccgtcgacg cggtcctcaa gcgcgagggc aaaacgctcg
     1321 ccacctacgg cctcgccagc ggcccgcagg gttaccgtcg cttgcgcgaa tttctcacca
     1381 ccaaactcaa gcgcgacgcc gggatcaact gcgtcgccga cgacatcctg atcgtctcgg
     1441 gctcgctgca ggcgctcgat ctcgtcaacg cgacgctgct ggcgcgtggc gacaccgtga
     1501 ttatcgagca ggacacctat cagggcgcgc tgaaccggtt gacccggctc ggcatcaatg
     1561 tggtcggcat tccgctcgac cgggagggca tgcggatgga ggcgctggcg gcggcgctgg
     1621 aggacctcaa gcgccgcagc atcacgccga aatacatcta caccatcccg accgtacaga
     1681 acccgaccgg caccatcatg ccggaggcgc gccggcacga actcctcaag ctcgccgaac
     1741 aatacgccgt gccggtgttc gaggatgatt gctacgccga cctgatctgg gacggcaagc
     1801 gcccgcccgc gctctatgcc atgaacacaa atggcggcgt gattcacatc ggctcattct
     1861 caaaatcgat cgcgccggcg ttgcgggtcg gctacatcgt cgcaccctgg gcggcgctgt
     1921 cgcggatgct ggcgctgaag accgacgccg gttccggcgc gctcgaacag atggtgctcg
     1981 cggaatattg cgcgccgcat ttcgcgacgc atgtgccgaa gctgacgcgt ggcctgcggg
     2041 ccaagctcga caccctgatg gaggcgctca acgcgcaatt cggcacatcg gccgaattcg
     2101 aagaccccaa gggcggtatc ttcctgtggg taaagctgcc ggacaatgtc gatacgctga
     2161 aactgtacca gtcagcactc gccgccggcg tcgccatcaa tccggg
//
LOCUS       DATNOI010000024         2106 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1943987,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000024 DATNOI010000000
VERSION     DATNOI010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1943987"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1248
                     /locus_tag="VN968_00795"
     CDS             79..1248
                     /locus_tag="VN968_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HXL27820.1"
                     /translation="MTASIVGWAHTPFGKLDTETVESLVVRVANEALADAGISAGDVD
                     EIVLGHFNAGFSPQDFTASLVLQADPQLRFKPATRVENACATGSAAVHRGIRAIAAGA
                     AKIVLVVGVEQMTRTPAAEIGRNLLRASYLPEDGDTPGGFAGVFGKIAQAYFQKYGDQ
                     SDALAMIAAKNHKNGVANPYAQMRKDFGYEFCRSESEKNPFVAGPLKRTDCSLVSDGA
                     AALVLTDTETAKSMGKAVNIRATAHAQDFLPMSKRDILQFEGCTVAWQRALQSAGIQL
                     SDLSFVETHDCFTVAELIEYEAMGLTPKGQGARAIKEGWTQKDGKLPVNPSGGLKAKG
                     HPIGATGVSMHVMTAMQLLGQAPEGMQIKDPKLAGIFNMGGAAVANYVSLLEPAK"
     gene            1505..>2106
                     /locus_tag="VN968_00800"
     CDS             1505..>2106
                     /locus_tag="VN968_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXL27821.1"
                     /translation="MGPLEGVRVIDMTTVLMGPYATQMLGDYGADVIKIETLDGDVTR
                     QIGPTRHPGMGPVFLNTNRSKRSICLDLKKPKGRDAVLRLIASADVLVYNVRPQAMAR
                     LQLGYDVVSKVNPRLVYAGVFGFGQDGPYAAKPAYDDLIQGATALPALMAQTSDGVPR
                     YVPNALVDRIVGLTAVGAICASLVHRDRTGRGQRVDIPMF"
BASE COUNT          411 a          648 c          672 g          375 t
ORIGIN      
        1 tgaaatccct gaaagcggcg tgaagtctgt ttcccgatag gctacaaaac cctccgaacc
       61 gaaacaggga gaacgaccat gacagccagc atcgtcggat gggcgcacac gccattcggc
      121 aagctcgata ccgaaaccgt cgagagcctc gtggtccgcg tggccaatga agcgctggcc
      181 gatgccggta tctcggccgg cgacgtcgat gaaatcgtgc tcggccattt caatgccggg
      241 ttctcgccgc aggattttac cgcatctttg gtgctgcagg cggacccgca attgcggttc
      301 aagccggcga cacgggtcga gaatgcctgc gcgacgggat cggccgcggt gcatcggggc
      361 atcagagcca ttgccgccgg tgcggcgaag atcgtcctgg tggtcggcgt cgagcagatg
      421 acgcggacgc ctgcggccga aatcggcagg aacctgctgc gcgcgtcgta tttgcccgag
      481 gacggagaca cccccggcgg tttcgccggc gtgttcggca agatcgcgca ggcctatttc
      541 cagaaatacg gcgaccagtc cgatgcgctg gcgatgatag ccgccaagaa ccataaaaac
      601 ggcgtcgcca atccctatgc gcagatgcgc aaggattttg gctacgaatt ctgccgctcc
      661 gagagtgaga agaatccgtt tgtggcgggg ccgctaaagc gcaccgattg ctcgctggtg
      721 tcggatggcg ctgcggcgct ggtcttgacc gacacggaga ccgcgaagtc gatgggcaag
      781 gcggtcaaca tcagggcgac cgcgcatgcc caggatttcc tgccgatgtc aaagcgcgac
      841 atattgcagt tcgaaggctg caccgtggcg tggcagcgcg cgctgcaatc ggccggcatc
      901 cagctttccg atctttcctt tgtcgagacc cacgactgct ttaccgtcgc cgaattgatc
      961 gaatatgaag cgatgggctt gacgccgaaa gggcagggcg cgcgtgcgat caaggaaggc
     1021 tggacgcaaa aagacggcaa gctgcccgtc aatccctccg gcgggttgaa agcaaaaggc
     1081 catccaatcg gcgccaccgg cgtctccatg catgtgatga ccgcgatgca actgttgggt
     1141 caggcgcccg agggtatgca gatcaaggac ccaaagctcg cgggtatttt caacatgggt
     1201 ggtgctgcgg tcgcgaacta cgtctcgctg ctggagcccg cgaagtaggc gcgggatttc
     1261 tcgcatgtcg tcccggcgaa cgccgggacc cataacccca ggtgcttgtt gctttgccga
     1321 ggcgtcgcct ctgccgatca attgcaactt cacggagtat gggtcccggc gttcgccggg
     1381 acgacgctca ttctggaatc ggcgcatacg gccatacgcc tgctcccctt gatcttccca
     1441 gcgccctgca gttacgtggc ccgagagaaa agcgcaaaag cgcgcatcgg gagcgaacga
     1501 aaccatggga cccctggagg gcgtcagggt catcgacatg acgaccgtgc tgatggggcc
     1561 ctatgccacc cagatgctcg gcgactatgg tgccgacgtc atcaagatcg agacgctcga
     1621 cggcgacgtc acgcggcaga tcggtccgac ccgtcatccc ggcatggggc cggtgttcct
     1681 caacaccaac cgcagcaaac gcagtatctg cctcgatctg aagaagccca aagggcgcga
     1741 tgcggtgctg cggctgatcg catcagccga cgtgctggtc tacaacgtcc ggccgcaagc
     1801 gatggcgcgc ctccagctcg gctacgacgt ggtctcgaag gtcaaccccc gcctcgtcta
     1861 tgccggcgtg ttcggcttcg gccaggatgg gccgtatgct gcaaaacccg cctatgacga
     1921 tctgatccag ggcgcgaccg cgctgccggc gttgatggcg cagaccagcg acggcgttcc
     1981 gcgctatgtg cccaatgcgc tggtcgatcg cattgtgggc ttaaccgccg tgggtgcgat
     2041 ctgcgccagc ctcgtgcatc gcgaccgcac cgggcgcggc cagcgcgtcg atattccgat
     2101 gttcga
//
LOCUS       DATNOI010000025        15754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1601147,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000025 DATNOI010000000
VERSION     DATNOI010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15754
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601147"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2033
                     /gene="kdpB"
                     /locus_tag="VN968_00805"
     CDS             <1..2033
                     /gene="kdpB"
                     /locus_tag="VN968_00805"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HXL27822.1"
                     /translation="AFAKLDPRLMIKSPVMFVVETVAALTTVIFLRDLVTGGENLGFT
                     FQIILWLWFTVLFANFAEAVAEGRGKAQAESLKKTRTESQAKLLTGPDKSYRLVAGAS
                     LKVGDLVLVEAGDNIPSDGEVVEGVASVNEAAITGESAPVIRESGGDRSAVTGGTQVL
                     SDWIRVRITAAQGSTFIDRMIRLVEGAERQKTPNEIALNILLAGLTIIFVFATVTIPS
                     YAAYAGGQISVVILVALFVTLIPTTIGALLSAIGIAGMDRLVRFNVLAMSGRAVEAAG
                     DVDTLLLDKTGTITLGNRQATAFRPVRAVTEQELADAAQLASLADETPEGRSIVVLAK
                     EKYGIRSRDMAELSATFIPFTAQTRMSGVDAGASSVRKGAVDAILNYIDGGGSRVVVS
                     GNAARILHPAMSEASRELHAIADEIAKAGGTPLAVAKDGRLLGIVQLKDIVKGGIRER
                     FAELRRMGIRTVMITGDNPMTAAAIAAEAGVDDFLAQATPEDKLKLIRDEQAKGKLVA
                     MCGDGTNDAPALAQADVGVAMNTGTQAAREAGNMVDLDSNPTKLIEVVEIGKQLLMTR
                     GALTTFSIANDVAKYFAIIPAMFLAFYPQLAVLNVMHLASPQSAILSAIIFNALIIIA
                     LIPLALKGVAYRAIGAGALLRRNLMIYGLGGVIIPFIGIKAIDLVVAALNLA"
     gene            2141..2746
                     /locus_tag="VN968_00810"
     CDS             2141..2746
                     /locus_tag="VN968_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HXL27823.1"
                     /translation="MLKEIRPAIIILVLLTAITGLAYPLAMTAIAGVIFPKQAQGSLI
                     ERDGKVVGSALIGQVFKDDKYFHGRPSATTAPDPADATRTVSAPYNAANSGGSNLGPT
                     SKALNDRVKEDVDKLKAENPSAPVPIDLVTTSGSGLDPDISPEGALFQVPRVARARNM
                     PEDRIRELVTANTQGRLAGLLGEPRVNVLALNLALDTAAAK"
     gene            2788..5511
                     /locus_tag="VN968_00815"
     CDS             2788..5511
                     /locus_tag="VN968_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HXL27824.1"
                     /translation="MANQRGNLEKRPSPDALLEAARRESDQTGRLKIFVGAAPGVGKT
                     YEMLQSAHAKLKAGVDVVVGVVETHGRAETEALLQGLEGLPRKRIVYKDQSIEEMDLD
                     ALIARHPTIALVDELAHTNAPGSRHPKRYLDVEELLSRGIDVYTAVNIQHIESLNDVV
                     AQITHVRVRETVPDSVFDRADAIELIDLTPDDLIQRLKEGKVYVPKQAERALEHYFSP
                     GNLTALRELALRRTAERVDEQLLTHMQANAIAGPWAAGERILVCLSEDPRAAGLVRYT
                     KRLADRLHAPWTALSIETRRSLQLNDEQRDRLADTLRLAEALGGEALTIPGVGRRIAD
                     DVINFAQANNVTQIIIGKSTRSWGFELMRGSVVHDLVRRAGNISVNVIAGDELPPGPA
                     TKTAVQTAARPEPFDPRPYLMALTIVAVGLGAAELIQPIFGIENVDLVFITAVVSVAA
                     RFGLWPSLLASIAASLCYNFFFLPPVYTFTITDPTNVAAFFFFMLIAILVSNVAARVR
                     TQADSAIGRVRTTESLYAFSRKLAGTATLDDVLWATAYQTALMLKVRVVLLLPEDGIL
                     TVKAGYPPEDELDKADLAAANWAWGNDRSAGRGSDTLPGAKRLFLPMRTGRGAIGVIG
                     IDDDKTGPLLTPDQRRLLDALMDQGALAIERVLLVEDMDRVKRTAESDRLRSALLTSI
                     SHDLKTPLASVLGAASTMRDLASGLTDTEKHDLLATVIDESERLNRFIANLLDMTKLE
                     SGAIVPNTARHDVSEIVGSVLRRASKILGHHQVSLELAADVPMLELDAVLFEQVLFNL
                     LDNAAKYAPADTTISIRSLRDRDRVALQIIDEGDGIPPAELETIFDKFYRAQKGDHVR
                     PGTGLGLAISRGFVEAMHGTISAANRTDRSGAVLTIKLPIPAASHALDTAA"
     gene            5508..6197
                     /locus_tag="VN968_00820"
     CDS             5508..6197
                     /locus_tag="VN968_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL27825.1"
                     /translation="MNATPIKVLVIDDEPPIRKLLRMGLSTQGYEILEAASGKIALEL
                     LVQNPALIILDLGLPDIQGHELLRMIRGRNDSVPIVVLSSRGDEAGKVQALDLGADDY
                     LTKPFGMDELLARMRAALRHQLQVHGERPVFRSGDLSVDLVRRIVKVADKDIKLSPKE
                     YELLRVLVQHAGKVLTHRFLLKELWDELTDAQYLRVYVRQLRQKIEADPERPQFVLTE
                     TGIGYRLRAAD"
     gene            6406..6618
                     /locus_tag="VN968_00825"
                     /pseudo
     CDS             6406..6618
                     /locus_tag="VN968_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561236.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
     gene            complement(6738..7610)
                     /locus_tag="VN968_00830"
     CDS             complement(6738..7610)
                     /locus_tag="VN968_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HXL27826.1"
                     /translation="MAVRAYWKGSLKLSLVSCPVLLYPATTTVDKTRFHMINKETGNR
                     LKQQMVDAETGDVVEGDQKGRGYELKKGEYVEVDKDELEAVQIESNHTIDIDGFVPKD
                     EIDTRYLNHPYYIAPDGKTGIDAFAVIRDAMQDQDRVALARIVLTNREHVIAIEPLGK
                     GLLGTTLRYPYELRDEDDYFDDIKSPKISKDMAELASHILDTKAAHFDPAKFKDEYEN
                     ALKTLVKRKAAGKPVKSAEREERPDNVISLMDALRQSLKGKASAKRSAHSPARRTTHR
                     LAKKAHRSPARKAS"
     gene            7734..10409
                     /gene="ligD"
                     /locus_tag="VN968_00835"
     CDS             7734..10409
                     /gene="ligD"
                     /locus_tag="VN968_00835"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HXL27827.1"
                     /translation="MSLRNLSTYRKKRDFEKTAEPSGEAAIAPSKQRRFVIQKHDATR
                     LHYDLRLEFDGVFKSWAVARGPSLDPHDKRLAVEVEDHPLDYGDFEGTIPKGQYGGGT
                     VQLWDRGYWESDDPERGFKKGDLKFTLQGDKLGGSWVLVRMRSDRDGGKRTNWLLIKH
                     RDDFAREGQANDILDADQSVASGRTMQQIAEGKGKAPKPFMTVKSGRGKADAVWNSNR
                     GEAAEARAHNKSAEPRSAPAAKQPSRGKKVSALPDFVAPELCISVDRPPAAEGWCHEI
                     KFDGYRVQLRVENGDAALKTRKGLDWTEKFQAIAKEGSALPDGLIDGEIVALDHNGAP
                     DFSTLQAALSDGKTDDLIFYAFDLLFADGEDLRALPLGERKARLKKLLEARAKNKGRL
                     IRYVEHFETGGDAILQSACKLSLEGIVSKKLGAPYHSGRSENWTKAKCRAGHEVVLGG
                     WKTTHGKFRSLMAGVYRGDHLAFVGIVGTGFGQDTVRRIMPALKAAASDKSPFGGKDA
                     PNKTRDVHWLKPELVAEIEFAGWTDGGNIRQAAFKGLRQDKPADEVKAEKPAMTRIAK
                     PIGKIAAKAGKAAGTSSETQLKSADSTVMGVVISKPNKELWPDGGDGEGATKLDLARY
                     FEAVGGWMIGHLKGRPCSVVRAPDGINGEKFFQRHAMSGTSSLLELVKVSGDRKPYLQ
                     IDRVEGLAAVAQIGGLELHPWNCAPDAPDVPGRLVFDLDPAPEVEFSDVIDAAKDMRQ
                     HLADVGLESFCKTTGGKGLHVVTPLAYGARDKVTWKEAKAFAQGVCQWMADEDPERYL
                     LNMSKKLRKGKIFLDYLRNDRMATAVAVLSPRAREGATVSMPLTWAQVRGDLDPKRYT
                     VRTVPAQFARTKAWEGYDDAASSIKAAIKQLSARK"
     gene            complement(10569..10733)
                     /locus_tag="VN968_00840"
     CDS             complement(10569..10733)
                     /locus_tag="VN968_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HXL27828.1"
                     /translation="MSIGTIILIILVIALLGGFSGIGGGPFYGTGYYGGGGLGLIVVI
                     LLILLLLGRL"
     gene            10888..11199
                     /locus_tag="VN968_00845"
     CDS             10888..11199
                     /locus_tag="VN968_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HXL27829.1"
                     /translation="MSAPLVVSIPHQLGREEASRRLKAGLTRAASSIPVLKVDEERWE
                     QNRMIFRVRALGQAASGHLDVEDDHVRLEVTLPWLLQRFAQVAQVAIRNRGNLLLTKR
                     S"
     gene            complement(11405..14215)
                     /gene="treY"
                     /locus_tag="VN968_00850"
     CDS             complement(11405..14215)
                     /gene="treY"
                     /locus_tag="VN968_00850"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HXL27830.1"
                     /translation="MRAAIPIATYRLQLTASFDFDAAAAIAPYLKALGITHLYASPFM
                     KARKGSTHGYDIVDHAKINPELGGDTGFERLSGVLKQYGLGLILDFVPNHVGVHFADN
                     PWWLDVLEWGPASPHAVSFDIDWELLPYRARGGVLLPIIGSSYGQALEKGEIELRYDA
                     DEGSFSVWYFEHRLPIAPERYSEILGAILREAGAEDSAAGKCMLGLASRHRGLRHPNR
                     KEAPAFKAELRAIAGAAEIIARGLGAYRAGPDRAAQVLALHHLLERQHYRLGHWRLAS
                     SDINYRRFFDVNTLAGLRVEDAGTFDAIHRLVKTLIADNKLQGLRLDHIDGLRDPVQY
                     FQRLRRLIRDAQGRDAKPFYMVIEKILGEHEQLHRFAGVDGTTGYEWLNSITQVLIDG
                     KGLKPLDEVWRQVSNMSPRLEPVLKDAKRRVLETLLTSEFTVLTRLLARIAGGHYSTR
                     DYSADSLRQALELYVLHFPVYRTYLTLADPSEHDRTLISETIEKARADWFAADEGIFE
                     FLRDALTMDLTKPGRPAHSASRVRRFALKVQQFTGPMMAKSLEDTAFYRYHRLLALNE
                     VGGDPSAQALSIDAFHEAMKLRAKEWPHGMTATATHDTKRGEDARARLMALAEIPGEW
                     ASAVARWKILNAPHLVTAGEMRAPSAAFEYMLYQVLLGAWPSGQRDDTFLERMQAYAL
                     KAAREGKQETSWLNPNETYEAGLKTFIARILDRSASVEFLDSLETLAQRVALLGALNS
                     FSQITLKATMPGVPDFYQGTEFWDLSLVDPDNRRPVNFAERATVLASVENPDWESLAQ
                     HWPDGHIKLAWTRHLLKLRTELADVFTHGDYQPLQVSGPHSDHVIAFARRLGRDAVII
                     AVAKSFAAFSQGGRVWPRAEAFDGALNLQGYSVEGMGHEAGATEVRLAALFLHLPAAV
                     LKARLEGALKPARKSSAKLASK"
     gene            complement(14215..>15754)
                     /gene="treZ"
                     /locus_tag="VN968_00855"
     CDS             complement(14215..>15754)
                     /gene="treZ"
                     /locus_tag="VN968_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HXL27831.1"
                     /translation="QPDDVSGPSEVIDHASYRWRASDWRGRPWQEAVVLEAHVGTYTP
                     EGTYRAMIGKLDHLVETGITALELMPLADFAGSRNWGYDGVLWYAPDSAYGRPDDLKA
                     LIDEAHLRGLMVFLDVVYNHFGPEGNYLGRYAPGFFTQVQTPWGSAIDYRVPQVRAFA
                     TLNALHWLGEYRFDGLRLDAVHAIPDLGEIPMLHDLSVAVGKLAAETGRHIHLVLEND
                     DNTASALDAGQEPPRGKYRAQWNDDYHHAWHVLLTRESHGYYADYQRSPLRDIARAIG
                     SGFVYQGEASAHRGGRLRGEPSGTLAPTAFVNFLQNHDQIGNRAFGDRLETIVGAAAI
                     EAALAITLLAPAVPMLFMGDEWGSKAPFPFFCDFAGELADAVRNGRRKEFAGAYAKYG
                     AGIPDPLDPSTFQAAILDWPSRSEQPGRQRLALIQNLLAIRRREIVPRLAGAAFGHAH
                     AADNGLLTADWRMGDGATLGLLANLSNQAIDHQGNQTGTLIWGGETSGLVPPWSVFWR
                     LEAR"
BASE COUNT         3049 a         4840 c         4971 g         2894 t
ORIGIN      
        1 cggccttcgc aaaactcgat ccgcggctga tgatcaagag cccggtgatg ttcgtcgtcg
       61 agaccgtcgc ggcgctgacc acggtcatct tcctgcgcga cctggtgacc ggtggcgaaa
      121 acctcggctt cacgttccag atcatcctgt ggctgtggtt caccgtgctg ttcgccaatt
      181 tcgccgaagc cgtcgccgag ggccgcggca aggcgcaggc ggaatcgctg aagaagacgc
      241 gcaccgaaag ccaggccaag ctgctgaccg gaccggacaa gagctaccgg ttggtcgcgg
      301 gcgccagcct gaaggtcggc gacctcgtgc tggtcgaggc cggcgacaac atcccgtccg
      361 atggagaagt ggtcgaaggc gtcgcttcgg tcaacgaagc cgccatcacc ggcgagtccg
      421 cgcccgtgat ccgtgaatcc ggcggcgacc gctcggcggt caccggcggc acgcaggtgc
      481 tgtccgactg gatccgcgtc cgcatcaccg cggcgcaggg ctcgaccttc atcgatcgca
      541 tgatcaggct ggtcgagggc gccgagcggc agaagactcc gaacgagatc gcgctcaata
      601 tcctgctcgc gggcctgacc atcatcttcg tattcgcgac ggtgaccatc ccgagctacg
      661 cggcgtacgc cggcggccag atatccgtcg tgatcctggt agccctgttc gtgacgctga
      721 ttccgaccac catcggtgcg ctgttgtcgg cgatcggcat cgccggcatg gaccggcttg
      781 tgcgcttcaa cgtgctggcg atgtcgggcc gcgcggtcga ggccgccggc gacgtcgata
      841 ccttgctgct cgacaagacc ggcaccatca cgctcggcaa ccggcaggcg acggcgtttc
      901 gccctgtccg cgccgtgacc gagcaggagc ttgccgatgc ggcgcagctt gcttcgctcg
      961 ccgatgaaac ccccgagggg cgttcgatcg tcgtgctggc gaaggaaaaa tacggcatcc
     1021 gcagccgcga catggcggag cttagcgcaa cgttcattcc gttcacggcg caaacccgca
     1081 tgagcggcgt cgatgcgggt gcatcgtcgg tccgaaaggg cgcggttgat gcgatcctga
     1141 attatatcga tggcggcggg tcccgcgtcg tggtgtcggg aaatgccgcg cgcattctgc
     1201 accccgccat gtccgaggct tcccgcgagc ttcacgcgat tgcggacgaa atcgcgaagg
     1261 ccggcggcac cccgctcgcg gtcgccaagg acggccgcct gcttggcatt gtccagctca
     1321 aggacatcgt taagggcggc atccgcgagc gcttcgccga actgcgccgc atgggcatcc
     1381 gcaccgtgat gatcaccggc gacaatccga tgaccgcggc ggcgatcgcc gcggaagccg
     1441 gcgtcgacga tttccttgcg caggcgaccc ctgaggacaa gctgaagctc attcgcgacg
     1501 agcaggccaa gggcaaactg gtcgcgatgt gcggcgacgg caccaatgac gcgccggccc
     1561 tcgcccaggc cgatgtcggc gtcgccatga acaccggaac gcaagccgcg cgcgaggccg
     1621 gcaacatggt cgatctcgat tccaatccga caaaactgat cgaggtggtc gagatcggca
     1681 agcaattgct gatgacccgc ggtgcattga ccacgttctc gatcgccaac gatgtcgcaa
     1741 aatattttgc gattatcccg gcgatgttcc tggcgttcta tccgcagctt gcggtgctca
     1801 acgtcatgca tctggcaagc ccgcaaagcg ccatcctgtc ggcgatcatc ttcaatgcgc
     1861 tgatcatcat cgcgttgatt ccgctggccc tgaaaggcgt ggcgtatcgg gcgatcggag
     1921 ccggcgcgct gctacgccgc aacctgatga tctatggcct cggtggcgtc atcattccgt
     1981 tcatcggcat caaggccatc gacctcgttg tcgccgcctt gaatctggcc taaccacccc
     2041 gtcatcgcga gccagcgaag caatccatgg cgctacaaag aaagagtgga ttgcttcgtc
     2101 gcttcgctcc tcgcaatgac agaagaaatg gagacttatc atgttgaagg aaattcgccc
     2161 cgccatcatc atcctggtcc tgctgacggc gatcaccggc ctcgcctatc ccctcgccat
     2221 gaccgccatc gcaggcgtga tctttccaaa gcaggcgcaa ggcagcctga tcgagcgaga
     2281 cggcaaggta gtcggctcgg ccctgatcgg gcaggtattc aaggatgaca agtacttcca
     2341 tggccggccc tcggccacga cagcgccgga cccggctgat gccaccagga ccgtttcggc
     2401 gccgtacaat gccgccaatt ccggcggctc caacctcggc ccgaccagca aggccctgaa
     2461 cgaccgggtc aaggaagacg tcgacaaact gaaagccgaa aatccttccg cgccggttcc
     2521 gatcgatctc gtcaccacgt ccggcagtgg cctcgatccc gatatctcgc cggagggcgc
     2581 gctattccag gtgccgcggg tcgccagggc gcgcaatatg ccggaagacc ggatccgcga
     2641 gctggtcacg gctaatacgc aaggccgcct tgcgggattg ctcggcgaac cgcgggtcaa
     2701 tgtgttggcg ctcaatctgg cgctggacac ggctgcagcc aaatagcgcg gcctaggcgg
     2761 catcaggcgt gaggactata ttgccgtatg gcaaatcaac gcggcaacct tgaaaaaaga
     2821 ccctcgccgg acgcgcttct cgaagccgcc cggcgagaaa gcgatcagac cggacggctc
     2881 aagatcttcg ttggcgccgc ccccggcgtt ggcaagacct acgagatgct gcagagcgcg
     2941 catgccaaac tgaaggctgg cgtcgatgtc gtcgttggcg tggtcgagac ccacggacgg
     3001 gcggagacgg aggcgctgct gcagggcctc gaaggcctcc cgcgcaaacg gatcgtgtac
     3061 aaggatcagt cgatcgaaga gatggatctc gacgccctga tcgcgcgcca tccaacaatc
     3121 gcgctggtcg acgaactcgc ccacaccaac gcacccggca gccgccaccc gaagcgctat
     3181 ctcgacgtcg aggaactgct ctcccgcggc atcgacgtct acaccgccgt caatatccag
     3241 catatcgaaa gcctcaacga tgtggtcgcc cagatcacgc atgtacgggt gcgcgagacc
     3301 gtgccggatt cggtgttcga ccgcgccgac gccatcgaac tgatcgatct cacgccggac
     3361 gatttgatcc agcggctgaa agagggcaag gtctatgtcc caaagcaggc cgagcgcgcg
     3421 cttgagcatt atttctcgcc gggcaatctg accgccttgc gcgagttggc gttgcggcgc
     3481 accgccgagc gcgttgacga gcaactcctg actcacatgc aggcgaatgc gattgccggc
     3541 ccctgggccg cgggcgagcg tatcctggtt tgcctcagcg aggatccgcg tgcggcgggg
     3601 ctggtgcgtt acaccaaacg gctggcggat cggctgcatg cgccctggac ggctctcagc
     3661 atcgagacgc ggcgcagcct gcaactaaac gatgagcaac gcgaccggct tgccgacacg
     3721 ttgcgtttgg ccgaggcgct gggcggcgag gcgctgacca ttcccggcgt cgggcgccgc
     3781 atcgccgacg atgtcatcaa tttcgcgcaa gccaacaacg tgacccagat catcatcggg
     3841 aagtcgacgc gttcctgggg gttcgaactc atgcgcggtt cggtggtgca cgatctggtc
     3901 cgccgtgccg gcaatatcag cgtcaacgtc atcgccggcg acgaactacc gcccgggcca
     3961 gctaccaaga ccgcggtgca gacggcggca cggcccgagc ctttcgatcc caggccctat
     4021 ctgatggcgc tgacgatcgt ggccgttggc ctcggggctg ccgaactgat ccagccaatt
     4081 tttggcatcg agaacgtcga tctggtattc atcacggcgg tggtgagcgt cgccgcccgc
     4141 ttcggcctgt ggccgtcgct gctggccagc atcgcggcgt cgctgtgcta taatttcttc
     4201 tttctgccac ctgtctatac cttcaccatc accgacccga ccaacgtcgc ggcgttcttc
     4261 ttctttatgc tcatcgcaat tctggtttcc aatgtcgcgg cacgggtgcg cacccaggcc
     4321 gactccgcga tcggcagggt gcgaaccacg gaatcgctgt acgccttcag ccgcaagctt
     4381 gcgggcaccg cgacgctgga cgatgtgttg tgggcgacgg cctatcaaac ggcgctgatg
     4441 ctgaaggtgc gggtcgtatt gctgctgccg gaggatggca tcctcacggt aaaggcgggc
     4501 tatccgcccg aagatgaact ggacaaggcc gatctcgccg ccgcgaactg ggcttggggc
     4561 aacgaccgct cggccgggcg agggtcggac acgctgccgg gcgccaagcg cctgttcctg
     4621 ccgatgcgga ccggacgcgg cgcgatcggc gtcatcggta tcgatgacga caagaccgga
     4681 ccgctgctga cgccggacca gcgtcgtctg ctcgatgcgc tgatggacca gggcgcgctt
     4741 gcgatcgaac gggtgctgct ggtcgaagac atggaccgcg tcaagcgcac cgcggagtcg
     4801 gatcggttgc gttcggcgct gctgacctcg atctcgcacg atctcaagac gccgctggcc
     4861 tcggttctcg gcgccgcctc cacgatgcgg gatctcgcca gcggcctgac cgataccgaa
     4921 aagcatgatc tgctcgccac cgtgatcgac gaatccgaac gcctcaatcg gttcatcgcc
     4981 aatctgctcg acatgaccaa gctggaatcc ggcgccatcg tgccgaatac ggcacggcac
     5041 gatgtcagcg agatcgtcgg cagcgtgttg cggcgcgcca gcaagatcct cgggcatcac
     5101 caggtctcgc ttgaacttgc ggctgacgtg ccgatgctgg agcttgacgc ggtattgttc
     5161 gagcaggtgc tttttaacct cctggacaac gcggccaaat acgcacccgc ggacaccacg
     5221 atatcgatca ggagtttacg ggatagggat cgggtcgccc tgcaaatcat cgacgaaggc
     5281 gacggcattc caccggcgga gctggagacc atattcgaca agttctatcg cgcgcaaaag
     5341 ggcgaccatg tccgtcccgg caccggtctc gggctagcga tctcccgcgg ctttgtcgag
     5401 gcgatgcatg gcacgatttc agccgccaac cgcaccgatc gaagcggcgc cgtgctgacc
     5461 atcaaattgc cgatcccggc ggcatcacac gcgctggaca ccgccgcatg aacgccacgc
     5521 caatcaaggt ccttgtcatc gacgatgagc cgccgatccg caaattgctg cggatggggc
     5581 tgagcacgca aggttacgaa atcctggaag ccgccagcgg caaaatcgcg ctcgaattgc
     5641 tggtccagaa tcccgcgctg atcattctcg atctcggcct tcccgatatc cagggccatg
     5701 aattgctgcg catgatccgc gggcgcaacg acagcgttcc gatcgtggtg ctgtcgagcc
     5761 gcggtgatga ggccggaaag gtccaggcgc tcgatctcgg cgccgacgat tatctgacaa
     5821 agccgttcgg gatggatgaa ctgctcgccc gcatgcgcgc ggcattgcgg caccaattgc
     5881 aggtccatgg cgagcgcccg gtgtttcgat cgggcgatct aagcgtcgat ctggtgcgcc
     5941 gtatcgtcaa ggtcgccgac aaggatatta aactgtcgcc gaaggagtac gaattgctgc
     6001 gcgtgctggt gcagcacgcc ggcaaggtct tgacccaccg ctttctctta aaggagctgt
     6061 gggacgaatt gaccgacgcg caatatctgc gggtctatgt ccgccagctc cggcagaaga
     6121 tcgaagccga tccggagcgt ccgcaattcg tgctcactga aaccgggatc ggctaccggc
     6181 tgcgcgcggc ggattaggca ccgaccgtca ccctgaggtg cgagtccttg cgagcctcga
     6241 aggatgacgg ttgggtgcgg tgcggcccat ccttcgagac gcgcgcaaag agcgcgctcc
     6301 tcaggatgac gtcgagcatg cggaagcgac ggcggccttg cagtttccta aaatggaacc
     6361 atctccaccc gcgaagatta ttttcccgca actgggagaa taatcatggc acgaaaatat
     6421 tcgaaaaagg catccgccaa agtcgggcgc gcgatgaaaa agcgcaaggc cggcactttg
     6481 aagagcggtc ggtcgggcaa gaaggtcaag agccgcgggc aggcgatcgc gatcggtctt
     6541 tccgaagcgc gggctgcggg agcgaaagtg ccgaagaagg cttccaagaa gacggccaag
     6601 aaaagcaaat cgaagaagta gcggcgacat tttactggtt tctcgtcatg gccgggcttg
     6661 tcccgcccat ccacgtcttt tttattgcga tgctgccaag acgtggatgc ccgggacaag
     6721 cccgggcatg acggcggtta actcgccttg cgcgccggcg agcggtgcgc tttttttgcg
     6781 aggcggtgcg tcgtccggcg cgccggggaa tgcgccgacc gttttgccga tgccttgccc
     6841 ttcaggcttt gccgcagcgc atccatcagg ctgatgacat tgtccggcct ttcctcgcgc
     6901 tcggctgatt tgaccggctt gccggcggcc ttgcgcttga ccagcgtctt caacgcattc
     6961 tcgtattcgt ccttgaattt tgcgggatcg aaatgcgcgg ctttggtatc gaggatgtgg
     7021 cttgcgagtt cggccatgtc ctttgagatc ttcgggctct tgatgtcgtc gaaataatcg
     7081 tcttcgtcgc gcaattcgta ggggtatcgc agcgtggtgc cgagcaggcc cttgcccaac
     7141 ggctcgatcg cgatgacgtg ctcgcggttg gtcaggacga tccgggccag cgcgacgcga
     7201 tcctggtcct gcatggcatc gcggatcacc gcgaaggcgt cgatcccggt cttgccgtcg
     7261 ggggcgatgt aataggggtg attgaggtag cgcgtgtcga tctcgtcttt cggcacaaag
     7321 ccgtcgatgt cgatggtatg gttgctttcg atctgaacgg cctccaactc gtccttgtcg
     7381 acctcgacgt attcgccctt tttcagctcg tatccgcggc ctttctgatc accttcgacg
     7441 acgtcgccgg tctcggcatc gaccatctgc tgtttcagcc ggttgccggt ttccttgttg
     7501 atcatgtgga agcgggtttt gtcgaccgtc gttgtggccg ggtaaagcag caccgggcat
     7561 gacacgaggg aaagctttag ggaccctttc caataggcgc gaacggccat tacaatctcc
     7621 ggcttgctat gggttaaacg aattcgggaa cttcaactga tattgcgggt tttggttccg
     7681 ggcccctgct gtcgtgccaa agggccgttt cgtcattggc gtgggatttg atcgtgtcct
     7741 tgagaaacct ctccacctat cggaaaaaac gcgatttcga aaagaccgcg gagcccagcg
     7801 gcgaagcggc gatagcccct tcgaagcagc ggcggttcgt gatccagaag catgacgcga
     7861 cccggctgca ttacgacctg cggctggagt tcgacggcgt cttcaaatcc tgggcggtcg
     7921 cgcgcggccc ctcgctcgat ccgcacgaca agcggcttgc ggtcgaggtg gaagatcatc
     7981 cgctggacta cggcgatttc gaaggcacca tcccgaaggg ccagtatggc ggcggcaccg
     8041 tgcagctctg ggatcgcggc tattgggaat ccgacgatcc ggagcggggg ttcaagaagg
     8101 gtgatctgaa attcacgctg cagggtgaca agctaggcgg cagctgggtg ctggtgcgaa
     8161 tgcgcagcga ccgcgacggc ggcaagcgca ccaattggct gctgatcaag catcgcgacg
     8221 attttgccag ggaaggccaa gccaacgaca ttctggatgc cgatcaatcg gtggcgtcgg
     8281 ggcgcacgat gcagcagatt gccgagggca agggcaaggc gccaaagccg ttcatgacgg
     8341 tgaagtccgg gcgagggaag gccgacgcgg tctggaattc gaaccgcggc gaagccgccg
     8401 aagcccgggc gcacaataag tcggccgaac cgcgatccgc gccggctgcc aagcagccgt
     8461 ccagaggcaa gaaggtgagc gcattaccgg atttcgtcgc accagaactc tgcatttccg
     8521 tcgatcgtcc gcccgccgcg gaaggctggt gccacgagat caaattcgac ggctaccggg
     8581 tgcaattgcg cgtggagaac ggtgacgcgg cgctgaaaac ccgcaaaggc ctggattgga
     8641 ccgaaaaatt ccaggccatc gccaaagagg gaagcgcgct tccggatggg ctgatcgatg
     8701 gcgagatcgt cgcgctcgac cacaacggcg cgccggattt ttcgacgctg caggccgcgc
     8761 tttccgacgg caagaccgat gatctgatct tttacgcgtt tgacctgctg tttgcggacg
     8821 gggaagatct tcgcgcgctt ccgctcggag aacgtaaggc gcgattgaag aaattgctgg
     8881 aggcgcgggc caagaacaaa ggccgactga tccgctatgt cgagcatttc gaaaccggcg
     8941 gcgacgccat cctgcaatcg gcgtgcaagc tgtcgctgga aggcatcgtc tcgaagaagc
     9001 tcggcgcgcc ctatcattcc ggccggtccg agaactggac caaggccaaa tgccgcgccg
     9061 ggcacgaggt cgtgctggga gggtggaaga ccacgcacgg caaattccgc tcgctgatgg
     9121 cgggggtcta tcgcggcgat catcttgcct tcgtcggcat cgtcggcacc ggttttgggc
     9181 aggacacggt ccggcgcatc atgcccgcgc tgaaggcggc ggcttcggac aagagcccct
     9241 tcggcggcaa ggatgcaccg aacaagaccc gcgacgtgca ttggctgaag cccgaactcg
     9301 tcgccgagat cgaatttgcc ggctggaccg acggcggcaa tatacggcag gctgcgttca
     9361 aagggctgcg ccaggacaag ccggccgacg aggtgaaggc ggaaaagccg gccatgacca
     9421 ggatcgccaa acccattggt aagatcgccg cgaaagccgg gaaggcagcg ggcacgtcga
     9481 gcgagacgca gctgaagtcg gcggatagca cggtgatggg cgtcgttatc tcaaagccca
     9541 acaaggagct gtggccggac ggcggtgacg gcgagggagc gacaaaactg gatctcgccc
     9601 gctatttcga ggcggtcggc ggctggatga tcggtcatct gaagggacgg ccctgttccg
     9661 tggtccgcgc tcccgacggc atcaatggcg agaaattctt tcagcgccac gccatgtccg
     9721 gcacctctag tctcctggag cttgtaaaag tctccggcga ccgcaagcct tatctgcaga
     9781 tcgatcgcgt cgagggcctt gcggcggtgg cgcagatcgg cggcctcgaa ctgcatccgt
     9841 ggaattgcgc gccggatgcg cccgacgtgc cggggcggct ggtattcgac ctcgaccctg
     9901 cgccggaggt cgaattttcc gacgtcatag atgccgccaa ggatatgcgg caacatctgg
     9961 cggacgttgg cctggagagt ttttgcaaga ccaccggcgg caaggggctg cacgtggtga
    10021 cgccgctggc gtacggcgcc agggacaagg tgacgtggaa agaggccaag gcgttcgcgc
    10081 aaggcgtttg ccagtggatg gccgatgaag atccggagcg ctatctgctc aacatgtcga
    10141 agaaactgcg caagggaaag atcttcctcg attatcttcg caacgaccgc atggccaccg
    10201 ccgtcgccgt gctgtcgccg cgggcccgcg aaggcgcgac tgtctcgatg ccgctgacat
    10261 gggcgcaagt gcgcggcgat ctcgacccga agcgctacac ggttcgcacc gtgccggcgc
    10321 agttcgccag gaccaaggcg tgggaaggct atgacgacgc ggcgtcatcg atcaaggctg
    10381 cgatcaagca gctgagcgcg cgcaaatgat taccacagtg ggagggatct ctgtgtcgtc
    10441 atgctccgcg aaaagctggt gttgacagcc tgtcgtgagg aagcgcagta cgtcacgtca
    10501 tgcgatgcgc aaaaaaatga gcgagattga gcatccctca atcccgcgcc attttgggct
    10561 acgaaaatct agagtcgtcc gagcagtagc agaatcagca gaatgacgac gatgagacca
    10621 agtccgccgc cgccataata gccggtgccg tagaacggtc cgccgccgat gccgctgaag
    10681 ccgccgagca acgcgataac aagaatgatc aggatgatag ttccgattga catgttcgtg
    10741 tgtctcctcc agcagttggc acccgttgct ctgcccgccg aaaaaacaaa cccgaaccca
    10801 taaggttccg tttttgcaac ccgcgccgct ctttttttcg aacgatggga aattacatgt
    10861 agttggatca acgtcaggat tttgagaatg tcagcacctc tcgtggtttc gattccgcat
    10921 cagcttggac gcgaagaagc gagccgccgt ctcaaggctg gattgacgcg cgccgcttcc
    10981 agcattccgg tgttgaaggt cgacgaggag agatgggagc agaaccgcat gatctttcgc
    11041 gtccgcgcac tggggcaggc ggcgtcaggt catctcgatg tggaggacga tcatgtccgc
    11101 ctcgaagtta cattgccctg gttgctgcag cgatttgcgc aagtggcgca agtcgcgatc
    11161 aggaatcgag gcaatctgct gttgaccaaa cggagttgac caacaccatc gatattgctc
    11221 tgcaacacaa aagctatggt gccatgcagc aattgcaaaa tctttttttg caactcattt
    11281 cgtaagcgtg ccggtaacga attctgcgca ggatcaaacc ctgcgcggga gaagggaacc
    11341 gacctttcac gggagtcggc acgacatccg ccaggccgcc cggccgtcga tcccgggcgg
    11401 ccttttattt cgaagcgagc ttcgcgctcg actttcgcgc cggctttaac gcaccttcga
    11461 gtctggcctt gagcaccgca gcgggcagat gcaggaacag agccgcgagg cgcacttccg
    11521 tcgcgccggc ctcgtgaccc atgccttcga cggaatagcc ttgcaggttg agagcgccgt
    11581 caaaggcttc cgcgcggggc cagacccgac cgccctgcga aaaagccgcg aatgatttcg
    11641 cgaccgcgat tatcaccgcg tcacggccaa ggcgccgcgc gaaggcgatg acgtgatcgc
    11701 tgtgcgggcc gctaacctgc agcggttgat aatcgccatg cgtgaacacg tcggcaagtt
    11761 cggtgcgcaa tttcagcaga tgccgggtcc aggcgagttt gatgtggccg tccggccaat
    11821 gttgcgcgag gctttcccaa tcgggattct cgaccgacgc cagcacggtg gcgcgttccg
    11881 caaaattcac cggccgccgg ttatcgggat cgaccagcga cagatcccag aactccgttc
    11941 cctgatagaa atcgggcacg cccggcatcg tcgccttcaa cgtgatctga ctgaacgaat
    12001 tcagcgcgcc cagcagcgca acacgctgcg ccagcgtttc cagcgaatcc aggaattcga
    12061 ccgaagccga gcgatccaga atccgcgcga taaaggtctt caatcccgcc tcgtaggttt
    12121 cgttgggatt gagccagctg gtttcctgct tgccctcgcg cgccgccttc aacgcatagg
    12181 cctgcatccg ctccagaaac gtatcgtcgc gttggcccga tggccacgcg ccgagcagca
    12241 cctgatacag catgtattca aacgccgcgg acggcgcccg catctcgcct gcggtgacga
    12301 ggtgcggcgc gttcaggatt ttccaccgcg ccacggcgct ggcccattca ccgggtattt
    12361 cggccaatgc catcaaccgc gcccgtgcat cctcgccgcg tttggtgtcg tgcgtcgcgg
    12421 tcgccgtcat gccgtgcggc cactccttgg cgcggagctt catggcctcg tggaatgcat
    12481 cgatcgaaag ggcctgggct gagggatcgc cgccgacctc gttgagcgcg agcaggcggt
    12541 ggtagcgata gaacgcggta tcctccagcg acttcgccat catgggaccg gtgaactgct
    12601 gcactttcaa ggcgaagcgg cgcacgcgtg acgcactatg cgcaggccga cccggcttgg
    12661 tcaggtccat ggtcaacgca tcgcgcagga attcgaagat gccctcgtcg gcggcgaacc
    12721 agtccgcgcg cgctttctcg attgtctccg aaatcaacgt gcggtcgtgc tccgacgggt
    12781 cggccaaagt cagataggtg cgatagaccg ggaagtgcag cacgtagagc tcgagcgcct
    12841 gccgcagact gtcggcggaa taatcgcggg tcgagtaatg tccgccggcg atccgcgcca
    12901 aaaggcgcgt cagcaccgtg aactcgctgg tcagcagggt ttcgagaacc cggcgtttcg
    12961 cgtccttcag gaccggctcc agcctgggtg acatattgct gacttgtcgc cacacctcgt
    13021 cgagcggctt gaggcctttg ccgtcgatca gcacctgggt gatgctattc agccattcat
    13081 agccggtggt gccgtcgacg ccggcgaagc gatgcagctg ttcatgttcg ccaagaatct
    13141 tttcgatcac catgtagaac ggttttgcat cgcgtccctg ggcgtcgcgg atcaggcggc
    13201 gcaggcgctg aaaatattgc acgggatcgc gtaggccgtc gatgtgatca agccgcaagc
    13261 cttgcagctt gttgtcggcg atcagcgttt tgaccaggcg atggatcgcg tcgaaggttc
    13321 cggcatcctc gacccgcaag ccggccagcg tgttgacgtc gaagaagcgc cgatagttga
    13381 tgtcgctgga ggctaatcgc cagtggccca gcctgtagtg ctggcgttcc agcagatgat
    13441 gcagggcaag gacctgcgcg gcgcgatccg gtcctgcgcg gtaggcgcct aatccgcgcg
    13501 cgataatttc agcggccccc gcgatagctc ttagctcggc cttgaaagcg ggcgcctctt
    13561 tccgattggg atgacgcaat ccccggtggc gcgaggcgag cccgagcatg cattttccag
    13621 ccgcgctgtc ctctgccccg gcttctcgca ggatcgcgcc caggatctcg ctgtagcgct
    13681 cgggggcgat cggcagccga tgttcgaaat accacacgga aaagctgcct tcatcggcat
    13741 cgtagcgaag ttcgatctcg cctttttcca gcgcctgccc ataggatgag ccgatgatgg
    13801 gcaacagcac gccgccacgc gcccggtacg gcaacagttc ccagtcgatg tcgaacgaca
    13861 ccgcatgtgg cgaggccggc ccccactcca gcacgtcgag ccaccacgga ttgtcggcga
    13921 aatgcacgcc gacatggttg ggcacgaaat cgagaataag gccgaggcca tactgcttca
    13981 gtacgccact caatcgttcg aagccggtgt cgccgccgag ttccggattg atcttggcgt
    14041 gatcgacgat gtcatagccg tgggtgctgc ccttgcgcgc cttcatgaag ggcgacgcat
    14101 agaggtgggt gatacccagc gctttcagat agggagcgat ggcggcagcg gcgtcgaaat
    14161 caaaactcgc ggtgagttgc aggcggtagg ttgcgatcgg aatggcggca cgcattatcg
    14221 cgcttccagg cgccagaaca ccgaccatgg cggaacgagg ccgctggtct cgccgcccca
    14281 gatcagggtg ccggtttggt ttccttgatg atcaatggct tgattcgaca ggttggccaa
    14341 aaggccaagc gtcgcgccgt cacccatgcg ccagtcggcc gtcagcaaac cattgtccgc
    14401 cgcatgcgca tggccgaacg ccgcgcccgc cagccgcgga acgatctcgc gccggcgtat
    14461 cgcgagcaag ttttggatca gcgcgagccg ctgacgtccc ggctgctcgc tgcgagatgg
    14521 ccaatcgagt atcgcggctt ggaaggtcga gggatccagc ggatcgggaa tcccggcgcc
    14581 gtatttcgca taggcgccgg caaattcctt gcgccggccg ttgcgtacgg cgtccgcaag
    14641 ctcgcccgcg aaatcgcaga agaacgggaa cggtgctttc gagccccatt cgtcgcccat
    14701 gaacaacatc gggacggccg gcgccaacaa cgtaatcgcc aaagcggctt cgatcgcggc
    14761 cgcgcctacg atggtttcga gccggtcacc gaatgcgcgg ttaccgatct gatcgtggtt
    14821 ctggaggaaa ttgacgaacg cggtgggagc gagtgtgccg ctcggctcgc cgcgcaatcg
    14881 cccgccgcga tgggcggagg cttcgccctg atagacaaag ccggagccga tcgcgcgagc
    14941 gatgtcgcga agcggtgaac gctgatagtc agcgtagtag ccgtgggatt cccgggtcag
    15001 cagcacgtgc caggcgtgat gataatcgtc gttccactgc gcgcgatatt tgccgcgcgg
    15061 cggctcctgg ccggcatcca gcgcgctggc ggtgttatcg tcattctcga gcaccagatg
    15121 gatgtgccgg ccggtttcgg ccgcaagctt ccccaccgcg acgctgagat catgcagcat
    15181 cggaatttca cccagatcgg ggatggcatg gaccgcatcc agccgcagcc cgtcgaagcg
    15241 atactcaccc agccaatgca gcgcattcag agtcgcgaag gcacgcacct gcggcacccg
    15301 gtaatcgatc gcgctgcccc agggcgtctg cacttgcgtg aagaagcctg gcgcatatcg
    15361 gccgagataa tttccttcgg gaccaaaatg gttatagacc acgtcgagaa ataccatcag
    15421 cccgcgcaga tgcgcttcgt cgatcagggc cttgagatcg tcgggccgtc cataggcgct
    15481 gtcgggcgcg taccacagca cgccgtcata accccaattg cgagaaccgg cgaaatccgc
    15541 cagcggcatc aattccagcg cggtgatacc ggtctccacc agatgatcga gcttgccgat
    15601 catggcgcgg taagtgcctt ccggtgtgta ggtgccgaca tgggcttcca gcacgacagc
    15661 ttcctgccac gggcgtccac gccagtccga ggcgcgccaa cggtagctgg catgatcgat
    15721 cacttcactg gggccggaca cgtcgtcagg ctga
//
LOCUS       DATNOI010000026         5019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2666631,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000026 DATNOI010000000
VERSION     DATNOI010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2666631"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VN968_00860"
     CDS             complement(<1..195)
                     /locus_tag="VN968_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HXL27832.1"
                     /translation="MAKVKSPTLRIATFADHKVITQPNPLRKVLRRVADKDLDDPIAR
                     AEKALAGLSGEFKNWMSVEAD"
     gene            complement(250..795)
                     /locus_tag="VN968_00865"
     CDS             complement(250..795)
                     /locus_tag="VN968_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL27833.1"
                     /translation="MFRIDFNKLRFLVCDDNPHMRRILRTLLHSFGAREVYESEDGAT
                     ALEMYSHYVPDIVITDWAMPIFDGLELAQMIRQPESKGNPYAPIIMLTGHSEKRRVTV
                     ARDAGVTEFLAKPISAKGLYQRILNVVANPRPFIKTKTYFGPDRRRNTSNAYIGPERR
                     VGGDAEVLQQPSLLDKARTSV"
     gene            complement(1064..1843)
                     /locus_tag="VN968_00870"
     CDS             complement(1064..1843)
                     /locus_tag="VN968_00870"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HXL27834.1"
                     /translation="MTTPKRYDRIAFLASAGAEAQQALAQLSALYGHHDPADADVVVA
                     LGGDGLMLQTLHQHMRSGKPIYGMHRGTVGFLMNEFSSRDLHARLAAARESVIHPLLM
                     RATDIHGVVHLHHAINEVALFRQTHQAARLRILIDERERMSELIADGIMVATPAGSTA
                     YNLSAQGPILPINAALLALTPICAFRPRRWRGALLPNTAFVVIEVLESDKRPVAAVAD
                     HDEARDVCRVEVLSDKTISMRMLFDPGHSLEERILSEQFGY"
     gene            2181..3734
                     /locus_tag="VN968_00875"
     CDS             2181..3734
                     /locus_tag="VN968_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HXL27835.1"
                     /translation="MTAAFAALRCARLALVLVAFSLAAGARADDAPPPQPAATPSGQK
                     AGGRGDAATPPAAAEQHRLPPDSTTKQTLALPGRTLDFTATAGSIRLFNDKGEPQADI
                     AYTAYQLDGAEPRNRPVTFVFNGGPGASSAWLQFGNVGPWRLPINAEAVTSSAAPDLM
                     PNAETWLDFTDLVFIDPVGTGYSRFVATGEDVRKRFFSIDGDVNSIAVTIRRWLEKFD
                     RLLSPKFVAGESYGGIRGPKIVRELQTEQGIGVKGLILISPVLDFREYSGSSLLQYVA
                     SLPTMAAVAREKKGSVTRADMADVEHYARGDFLGDLVRGPADTEATTRLADRVAALTG
                     IDQAVSRRLDGRFEVSEFRREFDRRNGKVTGRYDASVSGFDPYPDSSFHHFGDPSGDP
                     LTAPLTSAAVDVTTRKLNWRPDGSYQLLDGGVSKAWDFGRGLNPAESISQLRQILALD
                     PKLKLLVAHGLFDLATPYFGSKILLDQLPAYASPDRVKLVVYPGGHMFYSRDPSRQAF
                     RAEAEALMK"
     gene            complement(3820..4371)
                     /locus_tag="VN968_00880"
     CDS             complement(3820..4371)
                     /locus_tag="VN968_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL27836.1"
                     /translation="MNKSAIRLLTLAMYAMALIAVPLTTAYAAGGDSPSPPASDTKDT
                     KKVKKKKDKSSSIDDPKFLEGYRAAYATIYDRNDYSSAIEQLKALGQDDRADVANLIG
                     YSYRKLGDYKVSQIWYERALQADPDHVRTWQYYGLWQVEQGNRDSAQYHLNRIAALAG
                     TSSEEYRSLAAALEKPPGTGLVY"
     gene            4433..4648
                     /locus_tag="VN968_00885"
     CDS             4433..4648
                     /locus_tag="VN968_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27837.1"
                     /translation="MAPNIPGNDVNLRDFCGPHPRRARREHDEDVIQMNDVYHCASAW
                     VQRAARNVASMITSPAIPTGNLVIPAE"
     gene            4659..4955
                     /locus_tag="VN968_00890"
     CDS             4659..4955
                     /locus_tag="VN968_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27838.1"
                     /translation="MLKTISAALLAVSVLAAPVMAASSDKTAQAPATKSAQVKPSVQN
                     AKQAKPNALNANAKMGRHHRHPSHHRSHKKMTALKTHQVSKATIKHVTPAPKRG"
BASE COUNT          905 a         1584 c         1571 g          959 t
ORIGIN      
        1 gtcggcttcg accgacatcc agttcttgaa ttcgcccgac aggccggcga gcgccttttc
       61 cgcgcgcgcg atcggatcgt cgagatcctt gtcggcgaca cgccgcagca ccttgcgcaa
      121 cggattgggc tgcgtgatca ctttatgatc ggcgaaggtc gcgatccgca gcgtcgggga
      181 cttgactttc gccatcatgg tttccttggt gccttcgcgc tcaacggagg cgctctaaac
      241 gctggtgctc taaacagaag tccttgcctt gtcgagcagc gaaggctgct gcagcacttc
      301 cgcgtcgccg ccgacgcggc gctcggggcc gatataggcg ttgctggtat tgcgccggcg
      361 gtcggggccg aaataggtct tggtcttgat gaacgggcgc ggattggcga ccacgttgag
      421 aatgcgctgg taaagcccct tggccgagat cggcttggcg aggaattcgg tcacgccggc
      481 gtcgcgcgcc accgtgacgc gccgcttctc ggaatggccg gtcagcatga tgatgggggc
      541 gtagggattg cccttcgatt ccggttgccg gatcatctgg gcgagctcga ggccgtcgaa
      601 aatcggcatc gcccagtcgg tgatgacgat atcgggaacg tagtggctgt acatttcgag
      661 cgcggtggcg ccgtcctcgg actcatacac ctcgcgcgcg ccgaacgaat gcagcagcgt
      721 ccgcaggatg cggcgcatat gcggattgtc gtcgcagacg agaaatcgca gcttgttgaa
      781 gtcgatgcga aacatggtca ggctctaggg accaccgaag agttcggtat actccgcgtt
      841 aaccatatcc cgtccccgtt aacgaatggt tgcgaagggg gagggaacga cgttcgtata
      901 tcccggacct catcctgagg agcgcgccct tgcgcgcgtc tcgaaggatg gccgcgagtc
      961 cgcgctttgc gtccatcctt cgagacgctt gcttcgcaag ctcctcagga tgaggtcgga
     1021 gatatatttc acaactgcgt tcgccgtgtc cgacgatgga gccctaatag ccgaattgct
     1081 cgctcaggat gcgctcttcc aggctgtggc cggggtcgaa cagcatccgc atcgagatgg
     1141 tcttgtcgga aagcacctcg acccggcaga catcgcgggc ctcgtcgtga tcagccacgg
     1201 ccgcgaccgg ccgcttgtcg ctctcgagca cttcgatgac gacgaaggcg gtgttcggga
     1261 gcagggcgcc gcgccagcgc cgcgggcgga acgcgcagat cggggtcagc gccagcagtg
     1321 cggcgttgat cggcaggatc ggtccctgcg cggaaagatt ataggcggtg gatcccgctg
     1381 gcgtcgccac catgatgccg tcggcgatca gctccgacat gcgttcgcgc tcgtcgatca
     1441 ggatccttag gcgcgccgcc tgatgggtct ggcgaaacag cgccacctcg ttgatggcat
     1501 gatgaagatg caccacgcca tggatgtcgg tggcccgcat cagcaaggga tggatcaccg
     1561 attcccgcgc cgcggccagc cgcgcatgca gatcgcgcga gctgaactcg ttcatcagaa
     1621 aaccgacggt gccgcgatgc atgccgtaga tcggcttgcc ggaacgcata tgctgatgca
     1681 gcgtttgcag catcaatccg tcgccgccga gcgccaccac gacatcggca tcggcgggat
     1741 cgtgatggcc gtaaagcgcc gaaagctgcg ccagcgcctg ctgggcctcc gcgcccgcgc
     1801 tggcaaggaa ggcaatccgg tcatatcgct tgggcgtggt catgtgggct tagtggtcat
     1861 gtgggcttag ccttgggatt ttagccttgg gattttagcc ttgggattgc cggctcatct
     1921 gggcgctgga aaaggatcgg gggcgcgcga acgtcgtcta tacgaccttg gccgggcttg
     1981 tcgagatagg ctcgccagct tggcatgtca cgagatcgcg taatgcgccg gaaagcaaag
     2041 ctggctatca ctgcgcggcc gcgaaccatg ccaacttggc acgattttgg gccagaaccc
     2101 gcatcatcga ggaaatcctg gccgttagga ttaatgcggc cgtggcgggc cgatggcggg
     2161 aagccgggag gagaaccgat atgactgccg ctttcgccgc gctgcgctgt gcgcgcctcg
     2221 cgctcgtgct ggtcgcgttc tcgcttgctg cgggggcgcg cgccgacgat gcgccgccgc
     2281 cgcaacctgc cgcaacaccg tcgggtcaga aggccggtgg gcgcggcgac gcggccactc
     2341 cgcctgccgc cgccgaacaa catcgcctcc cgcccgactc gacaaccaaa caaacgctgg
     2401 cactccccgg gcgcacgctc gacttcacgg caacggcggg ctcgatccgc ctgttcaacg
     2461 acaagggcga gccgcaggcc gatatcgcct ataccgccta ccagcttgac ggcgcggagc
     2521 cgcgtaaccg gccggtgacc ttcgtcttca atggcggccc gggtgcgtcc tcggcgtggc
     2581 tgcaattcgg caatgtcggc ccctggcggc tgcccatcaa cgcggaggcg gtgacgtcct
     2641 ccgcggcgcc cgatctcatg cccaacgccg agacctggct cgacttcacc gatctcgtct
     2701 tcatcgatcc ggtcggtacc ggctacagcc gcttcgtcgc gaccggcgag gatgtccgca
     2761 agcgattttt ttcgatcgac ggcgacgtca actcgatcgc ggtgacgatc cgccgctggc
     2821 ttgaaaagtt cgaccgcctg ctgtcaccca aatttgtcgc cggtgaaagc tatggcggca
     2881 tccgcggtcc gaagatcgtt cgcgaattgc agaccgagca gggcatcggg gtgaagggcc
     2941 tgatcctgat ctcgccggtg ctcgacttcc gcgaatattc aggctccagt ctgctgcaat
     3001 atgtggcgag ccttccgacc atggcggcgg tcgcgcgcga gaagaagggc tcggtgaccc
     3061 gcgccgacat ggccgacgtc gaacattatg cgcgtggcga ttttctgggc gatctcgtca
     3121 ggggcccggc cgacaccgag gccaccacgc gtcttgccga ccgggtggcg gcattgaccg
     3181 ggatcgacca ggccgtcagc cgccggctgg acggccgctt cgaggtttcc gagttccggc
     3241 gcgaattcga ccgccgcaac ggcaaggtga cgggacgcta cgacgcgtcg gtctcgggct
     3301 tcgatcccta tccggattcg agcttccatc atttcggcga tccgtccggc gatcccctga
     3361 ccgcgccgct gaccagtgcc gcggtcgacg tcaccacgcg caagctcaat tggcggccgg
     3421 acggatccta ccagctattg gatggcggcg tcagcaaagc ctgggatttc ggccgcggtc
     3481 ttaatcccgc cgagtcgatt tcccagctgc ggcagattct ggcgcttgat ccgaaactga
     3541 agctgttggt ggcgcacggg ctgttcgatc tcgccacgcc gtatttcggt tcgaagatcc
     3601 tgctcgatca gctgccggcc tacgcttcgc ccgaccgcgt caagctcgtg gtctatccgg
     3661 gcgggcacat gttctattcg cgcgacccct cgcgtcaggc tttccgggcc gaagccgagg
     3721 cgctgatgaa gtgaagcggc gctaacaaaa caaagcgcct ggcaataaaa agccggcgca
     3781 acctgcaagg ccgcgccggc tgatttgtat ttgccgaact cagtaaacca ggccggtgcc
     3841 gggcggtttt tccagcgccg ctgccagcga gcgatattcc tcgctgctgg tgccggccag
     3901 agcggcgatc cggttcaggt gatactgcgc ggaatcgcgg ttgccctgtt cgacctgcca
     3961 caagccgtaa tactgccagg tccgcacatg gtccgggtcg gcctgcagcg cgcgctcata
     4021 ccagatctgc gaaaccttgt agtcgccgag tttgcgatag gagtaaccga tcagattcgc
     4081 cacatcggca cggtcgtcct ggccgagcgc cttcaattgc tcgatcgccg agctgtagtc
     4141 gttgcggtcg taaatcgtgg cataggccgc gcgatagccc tcaaggaatt tcggatcgtc
     4201 gatgctggag ctcttgtcct tcttcttctt tactttcttg gtgtccttgg tgtccgatgc
     4261 cggcggtgat ggactgtcgc cgcctgcggc ataagcggtc gtcagcggta cggcgatcaa
     4321 cgccatcgcg tacattgcca gcgtcaaaag cctgatcgca gacttattca tgcatctctc
     4381 ctgatttcca ttgggacgat agtacaccgg agcccgttga tttgaacacc ggatggcgcc
     4441 gaacattccc ggcaatgatg ttaatttgag ggacttttgc ggaccgcatc ctcgccgtgc
     4501 ccgacgggaa catgacgaag atgtcatcca gatgaacgat gtctatcatt gcgcttcagc
     4561 gtgggttcag cgcgcggcac gtaacgtcgc cagcatgatc acgagcccgg cgattccgac
     4621 cggcaacctc gtgatccccg ctgaatgagg agacaaccat gttgaagacc atttccgcag
     4681 cactgctcgc cgtttccgtt cttgccgctc ccgtaatggc agccagctcc gacaagacag
     4741 cgcaagctcc ggctaccaaa tccgcacagg tgaagccgag cgtccagaac gccaagcaag
     4801 cgaagccgaa cgcgctgaac gccaatgcca agatgggtcg ccatcaccgg catcccagcc
     4861 atcatcgctc ccataagaag atgaccgcgc tcaagaccca ccaggtctcg aaggccacca
     4921 tcaagcacgt tacgcccgcc cccaagcgcg gctaaactga tccgcgggcg tggcctaacc
     4981 acattgcccc ccgccgcgac cgtggatatc aggccggcc
//
LOCUS       DATNOI010000027        25993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1056895,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000027 DATNOI010000000
VERSION     DATNOI010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 25993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25993
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056895"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..263)
                     /locus_tag="VN968_00895"
     CDS             complement(3..263)
                     /locus_tag="VN968_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27839.1"
                     /translation="MLRKLTLTLVAAASLGAMALAPTAASAHWKGGGMGGGFGGFHHH
                     HFGPGFGIGYIGGGYGDDGCYVTRPVLTPFGYRLRTINVCGY"
     gene            complement(439..2175)
                     /locus_tag="VN968_00900"
     CDS             complement(439..2175)
                     /locus_tag="VN968_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL27840.1"
                     /translation="MASLSQQRLTRFIRGISVRQVRLASGLVLFAYLVSHFLNHALGN
                     ISMDALAAGVYYHVAFWQFPPVGMVFYASALVHTGLGIWALYERRQFRWKAIEPLQLV
                     LGLSIPALIITHIIGVRLSQTLFGHEKLYPQVFFAYWIVWPPKMWLMYAVMIIAWIHG
                     CIGLNFWLRMKPFYQIAGPFLLGAAVLVPTLAILGLYQGGRSVVDSDSVEWRAENLSP
                     DRVGTPAQQALLSSIEDYFLIGYLGLVGLVLLGKGARALHERRGGMITLSYGDGRTVR
                     VPKGLSVLEASLRNNVPHASVCGGRARCSTCRIRVIGDCASLPEPSQREAFVLNRVGA
                     SSDPAIRLACQLRPATDLSFFQIFLPQITAATLRTSSPARIGEERYLVSMFVDMRGST
                     RLAENRLPFDTVFIVNRFLGAVSQAVIECGGQPNQFVGDGQLALFGLNASPQTACRQA
                     LRAASMIAANVEELNQFLEHDLREPIRFGIGIHGGEVIIGDIGYRDHMVFTALGDAVN
                     VAARLQDMTKSLACEAILSEEVRVTAGLGADGLPEQEVAIRGRSEPMIVRTVINASAL
                     STLVNELDVVAA"
     gene            2641..4212
                     /locus_tag="VN968_00905"
     CDS             2641..4212
                     /locus_tag="VN968_00905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXL27841.1"
                     /translation="MTALRAVYASVIDLRTDEAYYWTWSKESVLSFLDHPPMIAWFIR
                     FGTAIFGDTNLGVRFAGIVAMLVTQLLLADIVRRVTHDARAVVIAVLMPEAALYYGLL
                     MAKVSPDVALIPFAVAMVWALIRLNESGDARWWLAAGVFAGLALLSKFTVVMLLPAVA
                     AFMLVPEWRRRWLTSPYPWAAALIAITVFSPALVWNAENDWASFKFQFVRATATHEVS
                     LRTVGDYIGLQFGLVGFILLPVVLSGLALTAWRGYRQGNAVAILLSTCVIVPFGYFFW
                     KSLTLRVGDTWPMFMWPIGFAAAAINIAMLPREGFPAWMIKSTVAWAHAAVVPGIAFV
                     VLVFLYYVAAPWNFIGKADPIGGEAGYEKVVDRAQAELQKTGATWIAATDYRTYAMLR
                     WYFRDRLPVIQINERGRFLGFRDPGMDSIRGHTGLYVAREPDNDNPLWASTTAVREPL
                     ERVERSWRGTVMDTYALEKITNWTPELSPPPDSPLFRWRVLAGDFSPPLPGGDRRFGV
                     AASTDDHQRPLGGAS"
     gene            4379..4903
                     /locus_tag="VN968_00910"
     CDS             4379..4903
                     /locus_tag="VN968_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXL27842.1"
                     /translation="MGPAGRGGKSAGRLGKRAHGVKKADAMDYISGFTLTNDVSAREI
                     QQREVASGSRFWTAKNMPGFAPVGPFILTMDEVADTDNLWVTCDVNGKRRLRANTGDY
                     LYKVADVLEHFSRYVVFEPGDLIAMGAPSGVAVGQPNAAELYLQPGDDMVISFEGLMS
                     LRTRIVAPAGASGK"
     gene            5008..5295
                     /locus_tag="VN968_00915"
     CDS             5008..5295
                     /locus_tag="VN968_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27843.1"
                     /translation="MGKIASAIVAATLAAAPLVLPAPARAENGQIAAGVVGGLIGGAL
                     LGGALAARPAPPPPVYYAPEPVYVDEPACRLVRERYWDGYGWQFRRVQVCN"
     gene            complement(5355..6386)
                     /locus_tag="VN968_00920"
     CDS             complement(5355..6386)
                     /locus_tag="VN968_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HXL27844.1"
                     /translation="MSERPRHRKPATFKLDDPGVTVIDPDDSSRPSRGAIHITPELEP
                     ALLPVPVEAPIVAARRGFRWGAVFWAAVGGLILLGLGLSTTRLIEDLFARSEGLGYLG
                     SALALAAALALVVVAAREAFGLARLATIEKMHLRAAATLLSDDRAESRTIVQDLLKLA
                     HQNPQLARARATLQEHADDIIDGADLIRLAERELMTPLDQEARRLISSAAQRVSIVTA
                     VSPRALIDVLFVFAASLRLIRQLARLYGGRPGALGMIRLMRHVIAHVAITGGMAASDS
                     LVQQVLGHGIAAKLSQRLGEGMLNGLLTARLGLAAIDVTRPLPFTALPRPALGDLAKD
                     LLRKRDAEE"
     gene            complement(6383..7852)
                     /locus_tag="VN968_00925"
     CDS             complement(6383..7852)
                     /locus_tag="VN968_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HXL27845.1"
                     /translation="MALSFSEIVEEARLSARALLDYSDHFFNPTVRLGVTGLSRAGKT
                     VFITALIHGLTRGGRFPIFEAFATGRIARARLAPQPDDAVPRFDYENHVRTLIEQRRW
                     PNSTVDISELRLVIDYQRQNGADRTLTLDIVDYPGEWLLDLPLLNKGFEQWSAESLAL
                     SRESPRARLAEPWHAHLATLSASGPEDEQAALAAARLFTEYLRACRDERFAMSLLPPG
                     RFLMPGNLAGSPALTFAPLDLPADSTAPDGSLWAMMRRRYEAYKDVVVRPFFRDHFSR
                     LDRQVVLVDALSAFNSGPEALHDLEAALAGILDCFRIGRSTLLSSLFRPRIDRILFAA
                     TKADHLHHSSHDRLEAVLRRTVNRAVAKAEDTGADIDVVALAAVRATREAQVQRGREK
                     LPSILGTPASGEVANGEAFDGETEVATFPGDLPADPEELFKGDSAFQGLSSATAEKSD
                     FRFLRFRPPHLESQDADGPMLPHIRLDRALQFLIGDRLQ"
     gene            8035..8730
                     /locus_tag="VN968_00930"
     CDS             8035..8730
                     /locus_tag="VN968_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HXL27846.1"
                     /translation="MTIFKLKQLASTSAHKVAGAVRWNYDRAELIADGVVHGIGVFGG
                     LIAATVLVVLTAVYATGFEVAVVSIYVAGLLAMLVLSATYNLWPISRAKWVLRRFDHS
                     AIYVLIAATYTPFITQMKDSIFAIALLIGVWCVAIVGIVIKLVFPGRFDRLAVGLYLA
                     MGWSGFMVYDTVVASLPPLTLWFIAAGGVLYTFGVIFHAWQRLRFQNAIWHAFVLLGA
                     ACHYTAVLDLVLA"
     gene            8755..9549
                     /locus_tag="VN968_00935"
     CDS             8755..9549
                     /locus_tag="VN968_00935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL27847.1"
                     /translation="MQVAGKVVVVTGGANGIGRALCEALHRAGAAKVVVADLDLAGAE
                     AVAASVDGAAFQCDVGQEKDILNVIEETERQFGPIALFCSNAGIGGGFDPLSENAGGT
                     SDEPFARSWAVHVMAHIYAARHLIPRMKARGEGYFLNTISAAGLLSQVGSPAYSTTKH
                     AAVGFAENLAISHKADGIKVSILCPQGVDTNMLRSIPKGPQSGDGDLTPEQVANDALA
                     GLAQETFLILPHPQVLGYMRRKTENYDRWISGMAKIQAKMREAYGK"
     gene            complement(9587..10060)
                     /locus_tag="VN968_00940"
     CDS             complement(9587..10060)
                     /locus_tag="VN968_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HXL27848.1"
                     /translation="MTAPDLTLIDDIRAASRQMVRELGFMKATLAASDYPPSAVHTIL
                     EIGARGPMTSVQLAEFLHLEKSSVSRMARKLIECGELREASDAEDARIKPLSLTAKGR
                     RTLTTIQDFARRQVSGALGRLTETERRVVHEGLVIYARALRADRITETRSVHPVA"
     gene            10144..10941
                     /locus_tag="VN968_00945"
     CDS             10144..10941
                     /locus_tag="VN968_00945"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXL27849.1"
                     /translation="MGEPLPANRSQGTTADRMKGKVCLVTGGGSGIGRATALKMAEEG
                     AAAVLIAGRREAEIEKAAAECRILGSQSLAIRTDVTRENDVERLVGTAVERYGRLDVA
                     FNNAGFQERRAPLEQQGNTVYDDVFDANVRSIFLCFRHQLPVMFARGQGRIVVNASVS
                     GLRNPNPGLSLYSASKAAAISMMRSAAMEAAPRGVGINAIAPGRVITDMMLSSGVGDM
                     SSVAAALPLRRMGRPEEVAEAVVWLLSDAASYVVGHVLAADGGFLAS"
     gene            complement(10948..12573)
                     /gene="mdlC"
                     /locus_tag="VN968_00950"
     CDS             complement(10948..12573)
                     /gene="mdlC"
                     /locus_tag="VN968_00950"
                     /EC_number="4.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoylformate decarboxylase"
                     /protein_id="HXL27850.1"
                     /translation="MSRKSTKSNGASVSVKHATLDLLRAFGIRKVFGNPGSTELPFLA
                     DWPDDIDYVLGLQEASVIGMADGYAQATRNAGFVNLHSAAGVGNALGNIYTAHRNQTP
                     LVITAGQQARSILPLQAFLYAERASEFPRPYVKYSVEPARAEDVPAAIARAYYVAMQP
                     PCGPTFVSVPIDDWTHQTQAIEARNVSRELGPDPEAMKSLVAALSASKRPALVVGPGV
                     DRAQAVDLMVRVAEKAKAVVWVSPFSARCSFPERHPLFAGFLHASPGQLSDALREHDL
                     VVVIGAPVFTFHVEGHAAIFDGATTLFQITDDPTAAAVSPRGNSIIATMKPALATLLE
                     LLPDAKRAMPAGRVLPPAPVAADPTPVEFLLHTLFGAMPDDAALVEEAPSHRPAMQKF
                     MPMRGQDSFYTMASGGLGYSLPASVGMAVGRPDIRTICLIGDGSAMYSIQALWTAAQR
                     KLPLTVVVINNAGYGAMRSFSQVMQVRNVPGLDLPGIDFVKIAEGMGCDAVRVSKSSE
                     LAPALKRGLAHDGTSLIEVMVDSAVPLLYAQKH"
     gene            complement(12711..13565)
                     /locus_tag="VN968_00955"
     CDS             complement(12711..13565)
                     /locus_tag="VN968_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HXL27851.1"
                     /translation="MTDQPDLDHLRQWIGRTEEASDIVTAHLVKGLRATLFMEIGQPK
                     PGDAAPWTVHWCLAPPVYPMSALSADGHPTRGGFLPPVPLPRRMWAGGELEFLEPLRV
                     GDETTRTSRIADVTMKTGSTGVLCFVSVEHLITTPRGVAIRERQDIVYRDISTAQPSA
                     PAKSAPPPPSAQHRESHMADPVLLFRYSALTFNGHRIHYDRDYVTKVEGYPGLIFHGP
                     MQAAFLVEFAAKLHGGAAPKKFSYRGVQPLFEGSEFSVNANDTGAGMELWTANSAGQP
                     TMKGTATW"
     gene            complement(13562..14692)
                     /locus_tag="VN968_00960"
     CDS             complement(13562..14692)
                     /locus_tag="VN968_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXL27852.1"
                     /translation="MLPLEGLIVIAVEQAVAAPFCSSRLADAGAHVIKVERPEGDFAR
                     GYDAAAKGQSSYFVWLNRGKDSVVIDLATRQGGQTLEELIAGADVLIQNLKPGSMDKL
                     GFSLERLRKDYPAPICCTISGYGDDGPYAHRKAYDLLIQAESGLASITGGPDGPSRVG
                     LSIVDIATGATAHAAILEALIARSQSGKGADVRISMFDVMADWLSVPLLNAEAGNPPQ
                     RMGLAHPSIAPYGVFRSRDGKDILISIQSEREWKKLCADVLGLPALPDDPRFANMVER
                     VRNRKLTDKAVGDSFATIARDDLLKRLSDADIAFAEVNTMADLAVRPHLRRIEVDTPA
                     GPVTYPAPAAIVVGQPRHYGAVPGIGDHTDVARSSHVRTKSS"
     gene            complement(14702..15871)
                     /locus_tag="VN968_00965"
     CDS             complement(14702..15871)
                     /locus_tag="VN968_00965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL27853.1"
                     /translation="MTATEPQDEFADIRDAVAKLCAQFPGEYWRKLDREMAYPSAFVD
                     ALTEAGYLSVLIPEEYGGAGLKLSAAAAILEEIQRAGCNGGGCHAQMYTMGTLLRHGS
                     EEQKAKWLPKIASGELRLQAFGVTEPTSGTDTSSLKTFARRDGNNGYVVNGQKIWTSR
                     AEYSDLMILLARTTPKEQSQKRTDGLSVFIVDMKAAKGNGLSISPIRTMMNHSTTEVF
                     FTDLLVPAENLIGEEGKGFRYILSGMNAERILIASECVGDAKWFIAKAAAYARERVVF
                     GRPIGQNQGVQFPIAKAYAAMRAAELMVKEATRKYEAGLDCGAEANMAKMLAADASWD
                     AANACVQTHGGFGFAEEYDVERKFRETRLYQVAPISTNLILSYLAEHVLGMPRSY"
     gene            15991..16170
                     /locus_tag="VN968_00970"
                     /pseudo
     CDS             15991..16170
                     /locus_tag="VN968_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(16167..16379)
                     /locus_tag="VN968_00975"
                     /pseudo
     CDS             complement(16167..16379)
                     /locus_tag="VN968_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613276.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(16361..16915)
                     /locus_tag="VN968_00980"
     CDS             complement(16361..16915)
                     /locus_tag="VN968_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HXL27854.1"
                     /translation="MDDVAIKHDARIGQGWPFQIYKTLKKFGVAQISYVPDAGHAELI
                     ELSHGDRAIQTTVLTTEEEGVALSAGAWLGGDRAVLLMQSSGVGNCVNMFSLAASCRL
                     PFLTLVTMRGEWAEFNPWQIPMGKATPQALEIIGVTVLRLDRAEDAEEVVSAAASLAF
                     ESDQQVAVLISQRMLGRKKWTETH"
     gene            complement(17118..17555)
                     /locus_tag="VN968_00985"
     CDS             complement(17118..17555)
                     /locus_tag="VN968_00985"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXL27855.1"
                     /translation="MSAALNPPSLAETINAKGFGHAAGFRIVAVEPGRAEVALACRDD
                     LLQFFGHFHGGVITALADQAAGIAVTSGLPAGRIGVTVEIKVNFLSPADGSELVARAK
                     TLKMSGSIGVATVEVFSRDGASETLCAFATATMRALDLPTKYR"
     gene            complement(17670..18944)
                     /locus_tag="VN968_00990"
     CDS             complement(17670..18944)
                     /locus_tag="VN968_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXL27856.1"
                     /translation="MNIRTSINADKAELLRLAREEAYSTPLKDFHPGAPRLFQDDTLW
                     PWFERLRKEEPVHYCTNSPIEPYWSVTKYNDIMHVDTNHGIFSSEVGLGGISIRDVPP
                     GYDQPSFIAMDQPRHSAQRKTVSPMFTPTHLDELAVLIRERAGKVLDTLPRNETFNFV
                     DRVSIELTTQMLATLFDFPWEERRKLTRWSDVSTALPKSMVVESAEQRRAEMDECAAY
                     FTKLWNERVNAEPKNDLISMMAHSGATRHMDPDNLMGNIILLIVGGNDTTRNTMSGSV
                     LALNENPDQYQKLRDNPALIDSMVPEVIRWQTPLAHMRRTALVDTEIGGKKIRKGDRV
                     VMWYVSGNRDEEGIENPNDFIVDRARPRTHLSFGFGIHRCVGMRLAELQLKIVWQEML
                     KRFDRIEVVGEPKRVYSSFVRGIESLPVRIPA"
     gene            complement(18977..20248)
                     /locus_tag="VN968_00995"
     CDS             complement(18977..20248)
                     /locus_tag="VN968_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXL27857.1"
                     /translation="MHGTIDLAQNSDQNAARERAYSIPLEQFDPGDPELFRSDTFWPY
                     FDRLRKEAPVHYCRDSVFGPYWSVTKYNDIMDIETNHSVFSSAASLGGITIRDIAPDL
                     RRESFIAMDQPRHGAQRKTVAPMFTPTHLDQLATSIRQRSAECLDNLPKNAVFDWVDR
                     VSIELTTQMLAVLFDFPWEDRRKLTRWSDVATTIPGPDGVVATEDARQVELQECATYF
                     GKLWSERANQPPKSDLLSMMVHSEATRDMDPKNFLGNLILLIVGGNDTTRNTLSGSVY
                     ALNKNPDQYGKLRANPALIDSFVPEVIRWQTPLAHMRRTALEDFEFRGRPIKKGDKVV
                     MWYVSGNRDEEVIERPYDFIIDRARPRTHISFGFGIHRCVGLRLAELQLKIIWEEILK
                     RFDKIEVVGEPKRVYSSFVKGYETLPVRIAG"
     gene            20478..21650
                     /locus_tag="VN968_01000"
     CDS             20478..21650
                     /locus_tag="VN968_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HXL27858.1"
                     /translation="MIPNAHRMFNFDLGETADAIRETVHDFASNEIAPRAAEIDKSNQ
                     FPRDLWPKLGALGLHGITVEEEYGGSGLGYLEHCIAVEEISRASAAVGLSYGAHSNLC
                     VNQLRRNGNEAQKRMFLPKLITGEHVGALAMSEPGAGSDVVSMSTRAEKKGDRYVING
                     SKMWITNGPIAETLVVYAKTDPAAGSRGITAFIIEKGMKGFSTTQKLDKLGMRGSDTG
                     ELVFENCEVPEENVLGEVGCGVNVLMSGLDYERSVLAAGPLGIMQACLDVVMPYVHER
                     KQFGEPIGSFQLVQGKIADMYTTMNASRAYVYAVAKACDRGETTREDAAGAILYASEK
                     ATQCALDAIQLLGGNGYINDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFEKSA"
     gene            21756..22880
                     /locus_tag="VN968_01005"
     CDS             21756..22880
                     /locus_tag="VN968_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HXL27859.1"
                     /translation="MSFVPPHPPPMPVSFSGDRGEFFGLVTRGAGLELVTVGFYRFWL
                     ATDIRRHLWSNTHIDGDSAEYTGRGRELLIGFLIALAILVPIYLAYFLVGLEAERYKA
                     FASIPLFLFFYLFGQFAIYRARRYRLTRTVWRGVRFWMSGSGWAYAGQAALWGLLMIL
                     TLGLILPWREAALERYKMRHTYYGDLQGSFEGRGWDFFKRGWWLWPLAPIALYVTPLA
                     PFVYAAFKAVEWRWWLSGIRFGDVRLESTMRRGALIGLYWKVIGWVLVLAVVFAAYLA
                     ACSGLVASMSGMPISQFFAPQVLLKSVPLLILAGLGYLVFALALNVVMRVYLLRDLWV
                     RALASTIVHDIEAAANVSARGEMANALGEGFADGLDVAGF"
     gene            22900..24015
                     /locus_tag="VN968_01010"
     CDS             22900..24015
                     /locus_tag="VN968_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXL27860.1"
                     /translation="MDSGPQNVSPSPGASGSAIYFDGTSSRRRLVTLAFNDGLELHED
                     GHRLAAWSFADIRRADSPSGALRVTCLTAPALARLEIRDAAVAAELISRCARLDENIP
                     GRHGVARIVGWSLAATVSIVGVVLFGVPLAADRLAPLVPRALERRLGDVAEVQVKTLF
                     GGKTCKNPSGQAAFTKLVDAIRESTGLDTSIQSEVLATPVPNAFALPGGRVYLFSGLL
                     AKAENADEIAGILAHELGHLSHRDGTRNLIYNGGTSFLIGLLFGDITGSGALIFASRS
                     LVTASYSREAEQNADTFAIDAMHRLGRSSKPMGELMFRITGKEGDKSLSVLSNHPLTE
                     DRLARMSQGDRPPSGPPLLTQSEWASLKAICSSDSKI"
     gene            complement(24016..24828)
                     /locus_tag="VN968_01015"
     CDS             complement(24016..24828)
                     /locus_tag="VN968_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2927 domain-containing protein"
                     /protein_id="HXL27861.1"
                     /translation="MSVNYRALLLRIFAVALAGIALEGGAWPPVAATEIPAIASRQRA
                     EKKTFTDSEITDGFFKTAFGAEYHLAGRVDRIRKYDAPVRVFAEGNRPDRKAQLAKIV
                     ADIAERIQHLDIAMAETSDGANVLVKLVRDRDLYRTITTFYGSERAREIRTSLDPQCL
                     SGFRKNENFEIEHSDVILTVDNGDFIFFDCGYEELLQSLGPINDTSSVPWTMFNDNVS
                     MGFFDVYDQYILNILYDPRVKAGMTVAEVKAVLPEVLADVRVWVRKVNNLAE"
     gene            complement(24939..25520)
                     /locus_tag="VN968_01020"
     CDS             complement(24939..25520)
                     /locus_tag="VN968_01020"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXL27862.1"
                     /translation="MARTFALLFAALTLLAGADRAHAQGFFGADSRDIMGGGPNFSIG
                     ASSPIPRQTVRFESRYTPGTILIDTAERRLYLVLPNGQALRYGIGVGRDGFRWGGVHR
                     ISAKKEWPSWTPPSQMLARRPDLPRHMAGGIDNPLGARAMYLGSTLYRIHGSNEPETI
                     GQAVSSGCFRMTNDDVADLYDRVSVGTTVVVKN"
     gene            25739..>25993
                     /locus_tag="VN968_01025"
     CDS             25739..>25993
                     /locus_tag="VN968_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXL27863.1"
                     /translation="MNGAESLVRTLVAGGVDVCFANPGTSEMHFVAALDRVEGMRCVL
                     GLFEGVVTGAADGYFRMKGTPASTLLHLGPGLANGLANLHN"
BASE COUNT         4706 a         7844 c         8392 g         5051 t
ORIGIN      
        1 gatcaatatc cgcagacgtt gatggtgcgc aggcggtagc cgaacggggt gaggacgggg
       61 cgggtcacat agcatccgtc gtcaccatag ccgccaccga tgtagccgat gccgaagccg
      121 gggccgaagt ggtgatgatg aaagccacca aagccgccgc ccattccgcc gcccttccag
      181 tgcgccgacg cggcggtggg agccagcgcc atcgcgccga gagaagctgc ggcaacaagt
      241 gtgagggtga gtttgcgtaa catggtcgtt ctccttgatg tgcgccttgg cgcgggttgg
      301 tcgtttcagt tctacccatc gcacatcagt cgtttcgctc aggagagggg ttcgagaaaa
      361 ttgcatttcg ttcgaggaca attttctgaa cgaatattca gatgtgaaaa acatcacata
      421 cttctggcgc gccgccgctc aggccgcgac gacatccaat tcgttaacca atgtggacaa
      481 cgcgctcgca ttgataacgg tgcggacgat catcggctcg ctgcgcccgc ggatcgcgac
      541 ttcctgctcg ggcaagccat cggccccaag tccggcggtg acgcgaacct cttccgagag
      601 gatcgcttcg caggcgaggc tcttggtcat gtcctgcagc cgtgctgcga cgttgaccgc
      661 gtcgccgagc gcggtgaaca ccatgtgatc gcggtagccg atgtcgccga tgatgacttc
      721 gccgccgtgg atgccgatgc cgaagcggat cggttcgcgc agatcatgct cgaggaattg
      781 attgagctcc tcgacattgg cggcaatcat gctggctgcc cgcagcgcct ggcggcacgc
      841 cgtttgcggg ctggcgttga gcccgaacag cgccaactgc ccatcaccga cgaactggtt
      901 gggctggccg ccgcattcga tcaccgcctg cgacaccgcg ccgaggaagc ggttgacgat
      961 gaacacggta tcgaacggca accggttttc cgcgagcctg gtcgagcccc gcatgtccac
     1021 gaacatgctg acgagatagc gctcctcgcc gatccgggcc gggctcgatg tccgcagcgt
     1081 ggccgcggtg atctgcggca gaaaaatctg gaagaaggag agatcggttg cgggcctgag
     1141 ctggcaggca aggcggatcg cgggatcgga actggccccg acccggttga gcacgaatgc
     1201 ctcgcgctgc gacggctccg gcagcgaggc gcaatcgccg atcacgcgaa tgcggcaggt
     1261 ggagcagcgg gctcggccgc cgcagacgct ggcatgcggg acattgttgc gcaggcttgc
     1321 ctcgagcacg ctgaggccct tcggcacccg aaccgtcctg ccatcgccgt acgacagggt
     1381 gatcatgccg ccgcggcgct cgtgcagggc gcgcgcgcct tttcccagca agaccagtcc
     1441 gaccaggccg agataaccga tcaggaaata atcctcgatg cttgacagta aggcctgttg
     1501 ggccggcgtg ccgacccggt ccggcgaaag attctccgcc cgccattcga cgctgtcgct
     1561 gtcaacaacg cttcgtccgc cctgatagag acccaatatg gccagcgtcg gaaccagcac
     1621 cgccgcgcca agcaagaacg gccccgcgat ttgatagaac ggtttcatcc ggagccagaa
     1681 attcagtccg atgcagccat gaatccaggc gatgatcatg accgcgtaca tcagccacat
     1741 tttgggcggc caaacgatcc agtaggcaaa aaacacctgc ggatagagct tttcgtgtcc
     1801 gaacagcgtc tggctgagcc gcacgccgat aatatgggtg atgatgagcg ccgggatgct
     1861 caggccgagc acgagctgca acggctcgat cgccttccag cgaaattgcc ggcgctcgta
     1921 aagcgcccag atcccgagac cggtgtggac cagtgccgag gcataaaaca ccatcccgac
     1981 cggtggaaat tgccagaacg cgacgtggta gtagacaccg gcggcaaggg catccatcga
     2041 aatgttgccg agcgcgtggt tgaggaaatg gctgaccaga taggcgaaca acaccagccc
     2101 ggaggcgaga cggacctgcc ggacgctgat gccgcggatg aatcttgtca gtcgttgttg
     2161 agaaaggctg gccatgcgat ccaatcgaac agtcatctac actagatgac gatagtagag
     2221 ttttattccg ccagcgatca atctttccag gcgttgtcgg cgcgaacgcc gggacctttc
     2281 ttcccctctc cccttgtggg agaggtggct cgaatgagcg tagcgaattc gagacgggtg
     2341 aggcggaccg gtctatcggt aagcgcggca acccctcatc cggcgcggac ttcgcgcagc
     2401 cgatgcaaag catcggcgtc ctcttaaagg acggcggccg aaggccgccg atggccacct
     2461 tctcccacaa ggggagaagg gaagaaagca cgcaacgatg gctgcctatg ccaacacgtt
     2521 gacaccctct ccggagtcag gaacaaagcg cccgtgacga ttgccgcccc cgatagcagc
     2581 acgaaaccgg acagtctggc ccttcggcgg ctgctcgcgg tggccgcatt gatcgtcgcg
     2641 atgacggcgc tgcgggcggt ctatgccagc gtcatcgatc tgcgcaccga cgaggcctac
     2701 tactggacct ggtcgaagga aagcgtgctt tcgttcctcg atcatccgcc gatgattgcg
     2761 tggttcatcc ggttcggcac cgcgatcttc ggcgatacca atcttggggt caggtttgcc
     2821 ggcatcgtgg cgatgctggt cacgcagctg ctactcgccg atatcgtccg gcgggtgacg
     2881 catgacgctc gcgctgtcgt gattgcggtg ctgatgccgg aagccgccct ttattacggg
     2941 ctgctgatgg ccaaggtctc gcccgacgtg gcgctgatcc cgttcgccgt cgcgatggtg
     3001 tgggcgctga tccggctcaa cgagagcggc gatgcgcgct ggtggctggc cgccggcgtg
     3061 ttcgcgggat tggcgctgct gtcgaaattc accgtggtga tgctgcttcc ggcggtggcg
     3121 gcattcatgc tggtgccgga gtggcgccgg cgctggctga caagcccgta tccgtgggcg
     3181 gcggcgctga tcgcgattac ggtgttctcg ccggctctgg tctggaacgc agagaacgac
     3241 tgggcgtcgt tcaaattcca gttcgtgcgg gcaaccgcga cccacgaagt gtcgctgcgc
     3301 acggttgggg attatatcgg cctgcagttc gggctggtcg gcttcatcct gctgccggtg
     3361 gtattgtcgg gtctcgcgct cacggcgtgg cggggatatc gccaagggaa cgccgtggcc
     3421 attctgctgt cgacttgcgt catcgtgccc ttcggatatt tcttctggaa atccctgacg
     3481 ctgcgggtcg gcgatacctg gccgatgttc atgtggccga ttggatttgc cgccgccgcc
     3541 atcaacatcg cgatgctgcc gcgcgaaggg tttccggcat ggatgatcaa atcgaccgtc
     3601 gcctgggctc atgctgcggt tgttcccggt attgccttcg tcgttctggt gtttctttac
     3661 tacgtcgccg cgccctggaa tttcatcggc aaggccgatc cgatcggcgg cgaggcgggt
     3721 tatgaaaagg tcgtggaccg ggcgcaggcc gaattgcaaa agaccggcgc gacctggatc
     3781 gcagccacag actaccggac ctacgccatg ctgcgctggt atttcagaga ccgtctgccg
     3841 gtgatccaga tcaatgagcg cggacgattt ttgggctttc gcgatcccgg catggactcg
     3901 atccgcggtc acaccggtct ttacgtcgcg cgcgagccgg acaatgacaa tccgttgtgg
     3961 gcttcaacga cagcggtcag ggagccgctc gaacgggtgg aacgcagctg gcgcggaacc
     4021 gtgatggata cctacgcgct ggagaaaatc accaactgga ccccggagct ttcgccgccg
     4081 ccggactcgc cgctgtttcg ctggcgcgtg ctggccgggg atttttcacc gccattgccc
     4141 ggcggcgatc ggcgctttgg tgttgccgct tcgacggacg atcatcagcg accactcggc
     4201 ggagcatcct gaaaatgttt tttcggattg cctctttggc tctctcggtt tgcgcgttag
     4261 cgctcccggc ttccgccgcc gattaccccg atcacgcaat atagatgatc gtgccgtttg
     4321 ccgccggcgg cggcaccgat gtgctggcgc gcatcatcgc gcaaaacctg aacagcaaat
     4381 ggggccagcc ggtcgtggtg gaaaatcagc cgggcgcctc ggcaagcgcg cccatggcgt
     4441 caagaaggcc gatgcgatgg attatatttc cggcttcacg ctcaccaacg atgtcagcgc
     4501 gcgcgaaatc cagcagcgcg aggtcgcctc cggcagccga ttctggaccg ccaagaacat
     4561 gccaggtttc gcaccggtcg gccctttcat tctgacgatg gacgaagtcg ccgacaccga
     4621 taatctgtgg gtcacctgcg acgtcaacgg caagcgacgg ctgcgcgcca acaccggcga
     4681 ttatctctac aaggtcgccg atgtgctgga gcacttttcg cgttatgtcg tgttcgagcc
     4741 cggcgacctc attgccatgg gggcgccgag cggtgtcgcg gtagggcagc ccaatgcggc
     4801 cgaactctat ttgcagcccg gtgacgacat ggtgatctcg ttcgaagggc tgatgtcact
     4861 gcgaacacgg atcgtcgcgc cggctggcgc ttccggaaaa taattttcct gcttgcacgc
     4921 agacttaaga aagcgttaat cgcgagatgc atgatggtcg gtatggcggc tcgtgaacga
     4981 tttcgcggct gcgattggag aaacgtgatg ggcaaaatcg cttcagctat cgtggctgct
     5041 accttggccg cggctccgct tgtgttgccg gcgcctgcaa gagccgagaa cggtcaaata
     5101 gccgctggcg tcgtcggcgg acttatcggc ggggcgctgc tcggcggcgc cctggcagca
     5161 cgtcctgcgc ctccgccgcc ggtctattac gcgccggagc cggtgtacgt cgatgaacca
     5221 gcttgccgtc tggtgcgcga gcgctactgg gacggatatg gctggcaatt ccgccgggtc
     5281 caggtttgca attgatcgca cattcctcgc ccaagaaatc ccgccatggg cgcgtcgtcg
     5341 cgcccggcta gaactcactc ctcggcatcg cgcttgcgca acaggtcctt ggcgagatcg
     5401 ccgagggcgg gccgcggcaa cgcggtgaag ggcagcggac gggtgacgtc gatggccgca
     5461 agcccaagcc gcgcggtgag cagtccgttg agcatgccct cgccaagccg ctgcgacagt
     5521 tttgctgcga tgccgtggcc aagcacctgc tggaccaggc tgtcgcttgc cgccatgccg
     5581 ccggtgatgg cgacatgcgc gatgacatga cgcatcagcc ggatcatgcc gagcgcgccc
     5641 ggccgtccgc cgtacaggcg cgccaactgc cggatcaggc gcagcgatgc cgcgaagacg
     5701 aacagcacgt cgatcaaggc gcgcgggctg acggcggtga cgatcgagac gcgctgcgcc
     5761 gccgacgaga tcaggcggcg cgcctcctga tcgagcggcg tcatcaactc gcgttccgca
     5821 aggcgaatca gatcggcgcc gtcgatgatg tcgtcggcat gttcctgcag cgtggcgcgg
     5881 gcccgcgcca gttgcgggtt ctggtgcgca agtttcaaca gatcctgcac gatggtccgg
     5941 ctttccgcac ggtcatcgct gagcagcgtc gcggcagcac gcagatgcat tttttcgatg
     6001 gttgcaagtc gcgccaggcc gaacgcctct cgcgcggcga cgacgacaag ggcgagcgcg
     6061 gcagcgaggg caagtgccga tccgagatag ccgagcccct cgcttcgcgc gaacagatcc
     6121 tcgatcagac gcgtggtgct cagcccgagc ccgagcagga tcaatccgcc aactgcggcc
     6181 cagaacaccg cgccccaccg aaagcctcga cgcgcggcaa cgatcggcgc ctcaaccggc
     6241 accggcagca gcgcaggctc gagttccggc gtgatgtgga tcgcgccgcg cgacgggcgg
     6301 cttgaatcgt ccggatcaat cacggtcacg ccggggtcgt cgagcttgaa ggtcgccggc
     6361 ttgcgatgac gggggcgctc gctcattgca gtctgtctcc gatcaggaac tggagggcgc
     6421 ggtcgaggcg gatgtgaggc aacatgggac cgtccgcatc ctggctctcg agatgcggcg
     6481 gccgaaaacg aagaaagcga aaatcgcttt tttccgcggt cgcgctggaa aggccctgaa
     6541 acgcgctgtc gcccttgaag agctcttcgg gatcggccgg cagatcgccc ggaaaggtcg
     6601 cgacctcggt ctcaccgtcg aaggcttccc cattggccac ttcgcctgat gcaggggtgc
     6661 ccaggatcga gggcagtttt tcacggccgc gctgcacctg ggcctcgcgg gtggcgcgca
     6721 ccgcggccag tgcaacgacg tcgatatcgg ctccggtgtc ctcggctttc gcgaccgccc
     6781 tgttgacggt tcgccgcagc accgcctcga gtcgatcgtg gctggagtgg tgcaggtgat
     6841 cggccttggt cgcggcgaac aggatgcggt cgatccgcgg ccggaacaga ctgctgagca
     6901 gcgtgctgcg gccgatcctg aagcaatcca gaattcccgc cagcgctgct tccagatcat
     6961 gcagcgcttc cggccctgag ttgaacgccg agagcgcatc gaccagcacc acctggcgat
     7021 cgagtctcga aaaatgatcg cgaaagaacg ggcgaaccac cacgtccttg taggcctcgt
     7081 aacgccgccg catcatcgcc cacagcgaac catcgggcgc ggtgctgtcg gccggcagat
     7141 cgagcggcgc aaaggtcagc gccggcgagc ccgcgaggtt tcccggcatc aggaaacggc
     7201 cgggcggcaa caggctcatg gcaaatcgtt cgtcgcggca ggcgcgcaaa tattcggtga
     7261 acagccgcgc ggcggccagc gcggcctgtt cgtcttcggg gcctgaggcg ctcaacgtcg
     7321 caagatgcgc gtgccatggc tcggcaagcc gcgcgcgcgg actctcccgt gacagggcga
     7381 ggctctcggc ggaccattgc tcaaaaccct tgttgagcag cggcaaatcg agcagccact
     7441 cgccgggata atcgacgatg tcgagcgtca gcgtgcggtc cgcgccattt tgccgctggt
     7501 agtcgatgac cagtcgcagt tcgctgatgt cgaccgtgga attcggccag cgccgctgct
     7561 cgatcaaggt gcggacatgg ttttcatagt cgaaacgcgg caccgcatcg tcgggttgcg
     7621 gcgcaagccg ggcgcgagcg attcgtcccg tcgcaaaagc ttcgaagatg ggaaagcggc
     7681 cgccgcgagt gaggccgtga atcagcgccg tgataaacac cgtcttgccg gcgcgtgata
     7741 gccccgtgac gccgagccgc actgttggat tgaaaaagtg atcgctatag tccagcaagg
     7801 cccgcgccga aagccgcgct tcttcgacta tttcggaaaa gctgagggcc atgcgggcgt
     7861 gaaaaactcg atctggtggg ggcaattggc tgcagaacaa cccgtaaagt ggcgattaga
     7921 gcgtcaaaat caagcttcac actccggacg ccattgcagg ctagggattg aacgttcgtt
     7981 gacctcgcga gacccatgtt ttatggcttc gcgtgtgaat tcgccgggag cgctatgacc
     8041 attttcaagc ttaagcaact cgcttcgaca tccgcccata aggtggccgg cgccgtgcgc
     8101 tggaattacg accgggccga actgatcgcg gacggtgtcg tgcatggaat cggcgtgttc
     8161 ggcggattga tcgcagcgac ggtgctggtc gtgctcaccg cggtttatgc cacggggttt
     8221 gaagtcgcgg tggtgtcgat ttacgtcgcg ggattgctgg ccatgctggt gttgtccgcg
     8281 acctataatc tgtggccgat ctcgcgggcc aaatgggtgc tgcgccgctt cgatcactcg
     8341 gcgatctatg tgctgatcgc ggcaacttac accccgttca tcacccagat gaaggacagc
     8401 atttttgcca tcgcgctgct gatcggcgtg tggtgcgtgg cgatcgtcgg gatcgtgatc
     8461 aagctcgtgt tcccgggccg tttcgaccgg ctggcggtcg gtctgtacct ggcgatgggc
     8521 tggagcggct tcatggtcta cgacaccgtg gtggcgtcgc tgccaccttt gacattgtgg
     8581 ttcatcgcgg ccggaggcgt gctctatacc ttcggcgtta tctttcatgc ctggcagcgg
     8641 ctgcgttttc aaaacgcgat ctggcatgcc ttcgtgctgc ttggcgcggc ctgccattac
     8701 accgccgtac tcgatctcgt tttggcctga gactttcaaa caactggtgt cacgatgcag
     8761 gtcgccggca aagtcgtggt tgtcaccgga ggcgccaacg gcatcggccg ggcgctctgc
     8821 gaagcccttc atcgtgccgg tgccgccaaa gtggttgtgg ccgatctgga tctggcgggt
     8881 gcggaggccg tcgccgcctc ggttgatggc gcggcgttcc aatgcgatgt cgggcaagag
     8941 aaagatattc tcaacgtcat cgaggaaacc gagcggcagt tcggcccgat cgcgctgttc
     9001 tgttccaatg ccggtatcgg cggcgggttc gatccgttat cggaaaacgc cggcggcacg
     9061 tccgacgagc cgtttgcgag gagctgggct gttcatgtca tggcgcacat ctatgccgcg
     9121 cgacatctga tcccgcggat gaaggcgcgc ggggaggggt atttcctcaa cacgatttcc
     9181 gccgccggac tgctgtcaca ggtgggaagt ccggcctatt caacgaccaa gcatgcggcg
     9241 gtgggatttg cggagaatct cgcgatctcg cacaaggccg acggcatcaa ggtttcgatc
     9301 ctgtgtccgc agggcgtcga taccaacatg ctccgctcga tcccgaaggg gccgcaatcc
     9361 ggcgacggcg atctgacgcc ggagcaagtc gcgaacgatg cgttggccgg actggcgcag
     9421 gaaacgttcc tgatcctgcc gcatccgcaa gtcttgggct acatgcgcag gaagaccgaa
     9481 aattacgatc gctggatatc aggcatggcg aagatccagg ccaagatgcg ggaagcctac
     9541 gggaagtgac gcgcgacacg catcccgggc tcgcaggaac cgaaattcag gcgaccggat
     9601 gcactgagcg agtttcggtg atgcgatcgg ccctcaatgc gcgcgcgtaa ataaccaatc
     9661 cctcgtgcac cactcgccgc tcggtctcgg tcagccgtcc gagcgcgccg gaaacctgcc
     9721 gtcgagcgaa gtcttgaatg gtcgtcagcg tacgtcgccc cttcgcggtc agcgacagcg
     9781 gcttgatccg ggcgtcttcc gcgtcggatg cttctctcag ttcgccgcac tcgatcagct
     9841 tgcgggccat gcggctgacg ctcgacttct caaggtgcag gaattcggcc aattgcaccg
     9901 aggtcatcgg gccgcgggcg ccgatttcca ggatggtatg gacggccgat ggcggatagt
     9961 cgcttgccgc cagcgtcgct ttcatgaagc cgagctcacg aaccatctgc cgggaagctg
    10021 cgcgaatatc gtcaatgagt gtcaggtcgg gggcggtcat cttgactccg atataatagt
    10081 tgtaccatac aactatatgc ccgtgcaacg ccgcatgcgg cgaggatcca ggagttgggc
    10141 gtcatgggtg agcctttgcc ggcaaacagg tctcagggca cgaccgcgga tcggatgaag
    10201 ggaaaagtct gtttggtgac gggcgggggc agcggcatcg gtcgtgccac cgccctgaag
    10261 atggcggaag agggcgcggc ggcggtactg atcgccggcc ggcgcgaggc cgagattgag
    10321 aaggccgcgg ccgagtgccg gatcctgggg tcgcaatccc tggcgataag gactgacgtc
    10381 acgcgggaaa acgatgtcga gcgcctggtc ggaaccgcgg tcgaacgcta cggccggctg
    10441 gatgtcgcgt tcaacaatgc cggtttccag gaaaggcgcg cgccgctcga gcagcaaggc
    10501 aatacggtgt acgacgacgt gttcgacgcc aatgtgcgct ccatcttcct gtgctttcgc
    10561 caccagctgc cggtcatgtt cgcgcgcgga cagggcagga tcgtggtcaa tgcgtcggtc
    10621 agcgggttac ggaatccgaa ccctggattg tcgctctatt ccgcctcgaa ggcggcggcg
    10681 atctcaatga tgcgctcggc ggcgatggag gcggcacccc gtggcgtcgg aatcaatgcg
    10741 atcgccccgg gccgtgtcat caccgacatg atgttgagct cgggggtcgg cgatatgtcg
    10801 agcgttgcgg cagcgctgcc gctgcggcgg atgggaaggc ccgaggaagt cgccgaagcg
    10861 gtggtctggc tgctttccga cgccgcgtca tatgtggttg gtcatgtgct ggcggcggac
    10921 ggaggttttt tggcctcctg agcgaggcta atgtttttgc gcgtagagca gcggcacggc
    10981 cgaatccacc atcacttcga tcaggctcgt gccgtcgtgc gcaaggccgc gcttgagcgc
    11041 gggcgcgagc tccgatgact tgctcacccg cacggcgtcg cagcccatgc cttccgcgat
    11101 tttcacaaaa tcgatgccgg gcagatcgag gccgggcacg ttgcgcacct gcatcacctg
    11161 gctgaacgat cgcatcgcgc cgtagccggc gttattgatc accaccaccg tcagcggcag
    11221 ttttcgctgc gccgcggtcc atagcgcctg gatcgaatac atcgccgagc cgtcgccgat
    11281 caggcagatg gtcctgatgt cgggacggcc gaccgccatg ccgaccgagg cggggaggct
    11341 gtagccaagg ccgccgctcg ccatggtgta aaaactgtcc tggccgcgca taggcatgaa
    11401 cttttgcatc gcggggcgat gcgacggggc ttcttcgacc agcgcggcgt cgtcaggcat
    11461 ggcgccaaat agcgtgtgca acaggaattc aaccggtgtc ggatcggcgg cgaccggcgc
    11521 gggtggcaga acgcggcccg ccggcatcgc gcgttttgca tcaggcagca actcgagcag
    11581 cgtcgccagc gcgggcttca tcgtcgcgat gatgctgttg ccccgcggcg atactgcggc
    11641 cgccgtggga tcgtcggtga tctggaacag ggtggtggcg ccgtcgaaga tcgcggcgtg
    11701 gccttcgacg tggaacgtaa acaccggtgc gccgatcacg accaccagat catgttcgcg
    11761 caaggcgtcc gagagctgac ctggtgaagc atgcaggaag cctgcgaaga gcggatgccg
    11821 ctccgggaaa ctgcagcgcg cggaaaacgg gctgacccag accacagcct ttgccttctc
    11881 cgcgacccgc accatcagat cgacggcttg cgcgcgatcg acgccgggcc cgaccacgag
    11941 ggcagggcgc ttgcttgccg aaagcgctgc gaccagcgac ttcatcgcct cgggatcggg
    12001 gccgagctcc ctgctgacgt tacgggcttc gatcgcctgg gtctgatgag tccagtcgtc
    12061 gatcggcacg gaaacaaaag tcggtccgca gggcggctgc atcgccacgt agtaggctcg
    12121 cgcgatcgcg gccggaacgt cttcggcacg cgcaggttcc acactgtatt tgacataggg
    12181 gcgcggaaat tccgacgcac gctcggcata caggaacgcc tgcagcggca gaatgctgcg
    12241 cgcctgctgg cccgcggtaa tcaccagcgg ggtctggttc cgatgcgcgg tatagatgtt
    12301 gccgagcgca ttgccgaccc cggctgccga atgaagattg acgaaaccgg cattgcgggt
    12361 ggcctgcgca tagccgtcgg ccatgccgat cacggatgct tcctgcaggc ccagcacata
    12421 gtcgatatcg tcaggccagt cggcgaggaa cggcaattcc gtcgagccgg gattgccgaa
    12481 cacctttctg attccaaagg cgcgcaacaa gtcaagcgtc gcatgtttga cgctgaccga
    12541 tgcgccgtta ctctttgttg atttgcggga caatgtcgtg tccttaggat tactcgaaat
    12601 gcctcaaccg tcactgcgag gagcgaagcg acgaagcaac ccatctattg gctatcgtat
    12661 ccggggatct ggattgcttc gcttcgctcg caatgacggt cacaaaaatc tcaccatgtc
    12721 gcggtgcctt tcatcgtcgg ctgccctgcc gaattcgccg tccacagttc catgccggca
    12781 ccggtgtcgt tggcattgac gctgaactcg ctgccttcga acagcggctg caccccgcga
    12841 tagctgaatt tcttcggggc cgcgccgcca tgcagcttcg ccgcgaactc caccaggaac
    12901 gccgcctgca tcgggccgtg gaagatcagg ccgggatagc cctcgacctt ggtgacgtag
    12961 tcgcggtcgt aatggatgcg gtggccattg aaggtcagcg ccgaataacg aaacagcagc
    13021 actggatcgg ccatgtggct ttcgcgatgc tgcgctgacg gcggcggtgg tgccgacttt
    13081 gcgggggcgg atggctgcgc ggtcgagata tcccgataga cgatatcctg ccgctcgcgg
    13141 atggccacgc cgcgcggtgt ggtaatcaga tgttcgacgg atacaaaaca cagcacaccg
    13201 gtgctgccgg tcttcattgt cacatcggca atgcgtgagg ttcgcgtcgt ctcgtcgccg
    13261 acacgcaagg gttcgaggaa ttcaagctcg ccgccggccc acatccggcg cggcagcggc
    13321 accggcggca ggaagccgcc gcgggtgggg tggccgtccg cactcaatgc cgacatcggg
    13381 tagaccggcg gcgccaggca ccaatgcacc gtccacggcg ctgcgtcacc cggtttcggc
    13441 tggccgatct ccatgaacaa agtcgcgcgc aaacccttca ccagatgcgc ggtgacgatg
    13501 tcggaggctt cttcggtgcg gccgatccat tgccgcaaat ggtcgagatc aggttggtcg
    13561 gtcatgacga tttggtccgg acatgagatg atctggcgac gtcggtgtga tcgccgatac
    13621 cgggcacggc gccgtagtgg cgcggttggc cgacgacgat ggcggccggc gccggatagg
    13681 tcaccggtcc cgccggcgtg tccacttcga tgcggcgcag atgcgggcgc accgcaaggt
    13741 cagccatggt gttgacctcg gcaaaggcaa tgtcggcgtc ggacagccgc ttcagcagat
    13801 cgtcgcgcgc tatggtcgca aagctgtcgc cgacggcctt gtcggtgagt ttgcgattac
    13861 ggacgcgctc gaccatgttg gcgaaccttg gatcgtcggg caaggcaggc aggcccagga
    13921 cgtccgcgca caattttttc cattcgcgtt cgctctggat cgaaatcagg atgtccttgc
    13981 cgtccctgga gcggaacacg ccgtagggcg cgatcgaggg gtgggcgaga cccatccgtt
    14041 gcggcggatt gccggcctcc gcattgagca gcggcaccga tagccagtcg gccatcacgt
    14101 cgaacatcga aatccggacg tccgcgccct tgccgctctg cgaccgcgcg atcaaggctt
    14161 cgaggatggc ggcatgcgcg gtggcgccgg tggcgatgtc gacgatggaa agaccgacgc
    14221 gcgacggacc gtccggaccg ccggtgatcg aggcgaggcc actctcggcc tggatcagga
    14281 gatcgtaggc cttgcgatgg gcataggggc cgtcgtcgcc ataaccgctg atggtgcagc
    14341 agatcggcgc cggatagtcc ttgcgcaggc gctccagcga gaagccgagc ttgtccatcg
    14401 agcccggctt gaggttctga atcagcacat ccgcgccggc gatcagctct tcgagggttt
    14461 ggcctccttg cctggttgcg aggtcgatga ccaccgaatc cttgccgcga ttgagccaga
    14521 cgaaatagct gctctggccc ttggccgcgg catcgtagcc gcgcgcgaaa tcaccctcgg
    14581 gccgctccac cttgatgaca tgggcgccgg catcggccag ccgcgagctg cagaacggtg
    14641 cggcgaccgc ctgttcgacg gcaatgacga tcagtccttc gagcggcagc attttttctt
    14701 ctcagtagga acggggcatg cccagcacgt gctcggcgag gtacgacaga atgaggttgg
    14761 tcgagatcgg cgccacctga tagagccggg tctcgcggaa tttgcgttcg acgtcatatt
    14821 cctccgcgaa gccgaagccg ccatgggtct gtacgcaggc gttggctgcg tcccaggagg
    14881 catcggccgc cagcatcttg gccatattgg cctcggcgcc gcaatcgagc ccggcctcgt
    14941 atttacgggt ggcctccttc accatcagct cggcggcgcg catcgccgca taggctttgg
    15001 cgatcggaaa ctggacgccc tggttctggc cgatcggacg gccgaatacc acgcgctccc
    15061 tggcgtaggc ggcagccttg gcgatgaacc atttggcatc gccgacacat tcggacgcaa
    15121 tcaagatgcg ctcggcattc atgccggaga ggatatagcg aaagcccttg ccttcctcgc
    15181 cgatcaggtt ttccgccggc accagaagat cggtgaagaa cacttccgtg gtggaatggt
    15241 tcatcatggt gcggatcggg ctgatcgaaa gcccgttacc cttcgccgcc ttcatgtcga
    15301 cgatgaacac cgacaacccg tcggtgcgct tttgcgactg ctccttcggc gtggtgcgtg
    15361 ccagcaggat catcaggtcg gaatattcgg cgcggctggt ccagatcttc tggccattga
    15421 cgacatagcc gttattgccg tcgcgcctgg cgaacgtctt cagcgacgag gtgtcggtgc
    15481 cgctggtcgg ctcggtgacg ccgaaagcct gcagccgcaa ttcgccgctg gcgatcttcg
    15541 gcagccattt tgccttttgc tcctcgctgc cgtggcgaag cagcgtgccc atcgtataca
    15601 tctgcgcatg acagccgccg ccattgcagc ccgcgcgctg gatctcttcg aggatcgccg
    15661 ccgccgcgga cagttttagg ccggcgccgc catattcttc cgggatcaag accgagagat
    15721 acccggcttc cgtcagcgca tcgacgaacg cactgggata ggccatctcg cgatcgagct
    15781 tgcgccagta ttcgccgggg aattgggcgc acagcttggc gacggcgtcg cggatgtcgg
    15841 caaattcgtc ttgtggttcg gttgcggtca tttgaatgac tggcggcagc gttgcgttga
    15901 tggctgacgg gcttgcagtt gtgaaaccac cgtgaagtcc ctatgctgaa ttgttatagc
    15961 gtattaactt gcattccaag attggcaagc atggatttcc gacagctgag aacattcagt
    16021 tgcgttgcgg aactcgggag cctcagcaaa gcctccgaca ccttgcgggt agcgcagccg
    16081 gcgctcagcc gtcagatcaa gcttctcgag catgagctgc gcgccgaact ctttacccgg
    16141 aacggccgcg gcatggtact gacggaggca agtgcgatac cgaggccgac catcgccgcg
    16201 ccgcccatcg cgccccacaa atagaaattt ccgggatgct caccggcatc gaaaacatcg
    16261 taggaagacg atccgaggcc ggtgacgacc agcaaatctc cgcgatcaac cagcagtgcc
    16321 ttgaccgctt cgcgacggtc gagcgtgaaa ttctgcgcca tcagtgcgtc tccgtccatt
    16381 tcttgcggcc gagcatgcgc tgtgaaatga ggaccgcgac ctgctggtcg ctctcgaatg
    16441 caagcgaagc ggccgccgaa acaacctctt cggcatcttc agcccggtcg agcctgagta
    16501 cggtcacgcc gatgatttcg agggcttgtg gcgtcgcttt gcccatcggg atttgccacg
    16561 ggttgaactc cgcccattcg ccgcgcatcg tcacaagtgt gaggaaggga aggcgacaac
    16621 tcgctgcgag cgagaacatg ttcacgcaat tgccgacgcc gcttgattgc atcaagagca
    16681 ccgcgcggtc tcctccgagc caggcgcctg cgctcagcgc gaccccttcc tcctccgtcg
    16741 tcaggacggt ggtctggatg gcgcgatcgc catgcgacag ctcaatcagc tcggcgtggc
    16801 cggcatcggg gacataggaa atctgcgcta cgccgaactt tttcagcgtc ttgtagatct
    16861 ggaacggcca gccttggccg atcctggcat catgcttgat ggctacatcg tccatggtgg
    16921 cctcggtcgt ggttcacgtc tcaacgcgtg cccgcgcccc aagccttaga acagaaaccc
    16981 ctggcccgcc ttcttatctg gttcaagcag tcatgccgcg attgtatagg aggggcttcc
    17041 ccgtatgggg ctcttcgccc aatcgtttcg caacgcgccg cggacgtcgg cgaattcgtc
    17101 ttgttgttcc gttgcgctca tcggtacttg gttggcagat cgagggcgcg catggtggcg
    17161 gtagcgaatg cgcagagggt ttcggaagcc ccgtctctgc tgaagacttc gacggtggca
    17221 accccgatcg accccgacat tttcagggtt ttggcgcggg cgacaagttc gctgccgtcg
    17281 gccggggaga ggaaattgac cttgatctcg acagtgaccc cgatccggcc cgcgggcaag
    17341 cctgacgtaa cggcaattcc ggccgcctga tcggccagcg cggtgatgac gccgccgtgg
    17401 aaatggccaa aaaactgcag caggtcgtcc cggcatgcca aggcgacttc ggcgcgtccc
    17461 ggctcgacgg cgacgatgcg gaaaccggcg gcgtggccaa aaccctttgc gttgatggtc
    17521 tcggcgaggc tcggcgggtt cagggcggcg ctcaaggtgt tggtccgttc atggcggtat
    17581 gtcttgcatt cggcgaacgg cgcagatcgc ctttcccggc cccaccgatc tcagcacggt
    17641 gggcaggcag agcgcaagca tgcccgccat tatgctggaa tgcgtaccgg cagggattcg
    17701 atgccgcgca cgaaactgga atatacccgc ttcggctcgc ccaccacctc gatcctgtca
    17761 aagcgcttga gcatttcctg ccacactatc ttcagctgca gctcggcgag ccgcatgccg
    17821 acgcagcgat gaatgccgaa cccgaacgac agatgggtgc ggggccgggc gcgatcgacg
    17881 ataaagtcgt tggggttctc gataccctct tcgtcgcgat tgcccgagac gtaccacatc
    17941 acgacccggt cgcctttgcg gattttcttg cccccgattt cggtatccac cagcgcggtg
    18001 cgccgcatat gcgccagcgg cgtttgccag cgaatgacct ccggcaccat gctgtcgatc
    18061 agcgccggat tgtcgcgcag tttctggtac tggtcgggat tttcgttcag tgccagcacc
    18121 gagccgctca tggtgttgcg ggtggtgtca ttgccgccga cgatcagcag gatgatgttg
    18181 cccatcagat tgtcggggtc catgtgccgg gtggcgccgc tgtgcgccat catcgagatc
    18241 aaatcgtttt tcggctcggc attgacgcgt tcattccaca gcttggtgaa ataggccgcg
    18301 cactcgtcca tttccgcccg gcgctgttcc gccgattcca cgaccatgct tttgggcagc
    18361 gcggtggaga cgtcggacca gcgcgtcagc ttgcggcgtt cttcccaggg aaagtcaaac
    18421 aacgtcgcca gcatctgggt ggtcagttcg atcgacaccc gatcgacgaa attgaaggtc
    18481 tcgttgcgag gcagcgtgtc gagcaccttg ccggcgcgtt cgcggatcag aacggcgagt
    18541 tcgtccagat gcgtcggcgt gaacatcggc gacacggttt tgcgctgggc agagtgccgc
    18601 ggttgatcca tcgcgatgaa gctcggctgg tcatagcctg gaggcacgtc gcggatcgag
    18661 atgccgccga gaccgacttc cgaggagaag atgccgtggt tggtgtcgac atgcatgatg
    18721 tcgttgtatt tggtaaccga ccaatagggc tcgatcggcg agttggtgca gtaatgcacc
    18781 ggctcttcct tgcgcagccg ctcgaaccac ggccacagcg tatcgtcctg gaacagtcgc
    18841 ggcgcgccgg gatgaaaatc cttcagcggg gtggaatagg cctcctcacg ggccagccgc
    18901 aaaagctcgg ccttgtcggc gttgatggat gtccggatgt tcatcgttga tcgattccaa
    18961 attaggagcg cggcagtcag ccggcgatgc gaacaggcag ggtctcgtag cccttgacga
    19021 agctcgaata tacccgcttg ggctcgccga ccacctcaat cttgtcgaac cgcttcagga
    19081 tttcttccca gattatcttc aattgaagct cggccaatct gagcccaacg cagcgatgaa
    19141 tgccgaagcc gaaggagatg tgggtacgcg gccgggcccg gtcaatgatg aagtcgtagg
    19201 gacgctcgat gacctcctcg tcgcggttgc ccgagacgta ccacatcacg accttgtcac
    19261 ctttcttgat cggcctgccg cgaaactcga agtcctcgag cgcggtccgg cgcatatgcg
    19321 ccagtggcgt ctgccagcgg atcacctccg gcacgaaact gtcgatcagc gccggattcg
    19381 cgcgcagctt gccgtactga tccggattct tgttcaatgc atagacgctg cccgacagtg
    19441 tgttgcgggt ggtatcgttg ccgccgacga tcagcaggat caggttgccg aggaaattct
    19501 tcgggtccat gtctcgcgtg gcttcgctgt gaaccatcat cgacaatagg tcgctctttg
    19561 gcggctgatt ggcgcgctca ctccagagct tcccgaaata tgtcgcgcat tcctgcagtt
    19621 cgacctgtcg cgcgtcttcg gtcgcgacga cgccgtccgg gccgggaatt gtggttgcga
    19681 cgtcggacca gcgcgtcagt ttgcggcggt cttcccaggg gaaatcgaac agcacggcga
    19741 gcatctgggt ggtcagttcg atcgaaaccc tgtcgaccca gtcgaacact gcgttcttcg
    19801 gcagattgtc aaggcattca gccgaccgct gccggatgct ggtcgcgagt tgatcgagat
    19861 gtgtcggcgt gaacatcggc gcgacggtct tgcgttgcgc gccatggcgc ggctgatcca
    19921 tggcgatgaa gctttcgcgg cgcaggtcgg gcgcgatgtc gcggatggtg atgccgccga
    19981 gcgatgcggc cgacgagaac accgaatggt ttgtctcgat gtccatgatg tcgttgtatt
    20041 tcgtcaccga ccagtaaggc ccgaacacgc tgtccctgca ataatggacc ggggcttcct
    20101 tgcgaagccg atcgaaatag ggccagaacg tgtcgctgcg aaacaattcg ggatcgccgg
    20161 gatcaaattg ctcaagcgga atcgaatagg ctcgctcccg cgcggcattt tgatcggaat
    20221 tttgagcgag atcgatcgtc ccatgcatcg ggtaactccc tgattcctcc gtggttgatt
    20281 ccacagcgtg ttccttggct gcgaattaca tcccgttgcg tggggcagag caaggacagt
    20341 ttgacgcctt ccggcgcgtc aaagtagcat tccggccggc gcaccctttt cagcatattc
    20401 ggcccgtgac aagcctggtt ccagagaata aggctgtgca gggaaagcag cgtttattgc
    20461 ccggaggaca cccgcccatg atccccaacg cgcatcggat gttcaatttc gatcttggcg
    20521 aaactgcgga tgcgattcgc gagaccgtgc atgatttcgc gagcaatgag atcgcgccgc
    20581 gcgccgccga gatcgacaag agcaatcaat tcccccgcga tctctggcca aaactcggtg
    20641 cgcttggcct gcacggcata accgtcgagg aagagtatgg cggatcgggc ctgggctatc
    20701 tcgagcactg catcgcggtc gaggagatct cgcgggcgtc ggcggcggtc ggcctgtcct
    20761 acggcgcgca ttcgaacctt tgcgtcaatc agttgcgccg caatggcaac gaggcgcaga
    20821 agcgaatgtt tctgccgaag ctgattaccg gtgaacatgt cggggcgctg gcgatgtcgg
    20881 agcccggcgc cggctccgac gtggtgtcga tgtcgacccg cgccgagaag aagggcgacc
    20941 gctacgtcat caacggcagc aagatgtgga tcaccaacgg cccgatcgcg gaaaccctgg
    21001 tggtctatgc caagaccgat cccgctgccg gctcgcgcgg catcaccgcc ttcatcatcg
    21061 aaaaaggcat gaagggattt tcgaccacgc aaaagctcga caaactcggc atgcgcggct
    21121 ccgacaccgg cgaattggtg ttcgagaact gcgaagtgcc ggaggaaaac gtgctcggtg
    21181 aggtcggctg cggcgtcaat gtgctgatgt ccggcctcga ctacgagcgc tcggtgctgg
    21241 cggcggggcc gctcgggatc atgcaggcct gcctcgacgt ggtgatgccc tatgtccacg
    21301 agcgcaagca gttcggcgag ccgatcggca gcttccagct ggtgcagggc aagatcgccg
    21361 acatgtacac cacgatgaat gcgtcccgcg cctacgtcta cgccgtcgca aaagcctgcg
    21421 accgcggcga gaccacgcgc gaggatgcgg ccggcgccat tctctatgcc tcggaaaagg
    21481 cgacacaatg cgcgcttgac gcgatccagc tgctcggcgg caacggctat atcaacgact
    21541 atcctacggg gcggctgttg cgcgacgcca agctttacga gatcggcgcc ggcaccagcg
    21601 aaatccggcg tatgctgatc ggacgggagc tgttcgaaaa atccgcatag gttcggtcga
    21661 accggatcgt attttgaggc gacaaaagcg gtaatatgtt aacttcattc ctgcctgccg
    21721 atttacttgc cttggggagt tgatccgtga atcagatgag ttttgtgccg ccgcatccgc
    21781 cgccgatgcc ggtgtcgttt tcgggcgatc gcggtgaatt tttcggactg gtgacgcgcg
    21841 gcgccgggct cgaactcgtc accgtcggtt tttatcggtt ttggctcgcc accgacattc
    21901 gccgccattt gtggtcgaac acccatatcg acggcgattc ggcggaatac accggccgcg
    21961 gtagggagtt gctgatcgga tttctgatcg cgctggcgat cttggtgccg atctatctcg
    22021 cctacttcct cgtcggcctg gaagcggaac gctacaaggc gttcgccagt atacccttgt
    22081 tcctcttttt ctatttgttc ggccaattcg cgatctaccg cgcgcggcgc taccgcctga
    22141 cccgcacggt gtggcgcggg gtgcgattct ggatgagcgg atcgggctgg gcctatgcgg
    22201 ggcaagccgc gctctggggg ctgctgatga tactcaccct cggcctgata ttgccatggc
    22261 gtgaggccgc cctcgaacgc tacaagatgc ggcataccta ttacggcgat cttcagggca
    22321 gttttgaagg gcgcggttgg gatttcttca agcgcggctg gtggctatgg ccgctggcgc
    22381 cgatcgccct ctacgtgacg ccgctcgcac ccttcgtcta tgccgcgttc aaggcggtgg
    22441 agtggcgctg gtggctgtcg ggcatccggt tcggcgatgt gcggctggaa tcgacgatgc
    22501 ggcgcggcgc cctgatcggc ctttactgga aggtcatcgg ctgggttctg gtgctggccg
    22561 tggtgtttgc cgcctacctg gcagcatgct cgggtctggt ggcgagcatg agcggaatgc
    22621 cgatcagtca gttcttcgcg ccgcaggtgc ttttgaagag cgttccgttg ctgatcctgg
    22681 cgggactggg ttatctggtg ttcgcgctgg cgctcaacgt ggtgatgcgg gtttatctgt
    22741 tgcgcgatct ctgggtgagg gctctcgcat cgacgatcgt ccacgacatc gaggcggcgg
    22801 cgaatgtttc cgcaaggggc gaaatggcca atgcgctggg cgagggcttt gccgacgggc
    22861 tggatgtcgc cggcttttag cgcatgagca tgtgtcacaa tggatagcgg gccgcaaaat
    22921 gtgtcgccgt cgccgggagc gagcggttcg gcgatttatt tcgacgggac gtccagtcgc
    22981 cgccgtttgg tgacgctggc gttcaacgat gggcttgagc ttcacgagga tggacacagg
    23041 ctggcggcat ggtcttttgc cgatatccgc cgcgccgaca gtccatccgg tgcgctccgc
    23101 gtgacttgcc tgacggcacc ggcgctggcg cggcttgaaa tccgcgatgc ggcggttgcc
    23161 gcggaactaa tctcccgctg cgcccggctc gatgagaata ttcccggccg gcacggcgtc
    23221 gcgagaattg tcggctggtc attggcggcg acggtttcga tcgtcggcgt ggttctgttc
    23281 ggtgttccgc tcgccgcgga ccgtctggcg ccgctggtgc cgcgggcatt ggaacggcgg
    23341 cttggcgatg tcgcggaagt ccaggtcaag acgctgttcg gcggcaagac ttgcaaaaac
    23401 ccgtcgggac aggctgcctt caccaagctc gtcgatgcca ttcgcgagtc aaccggcctg
    23461 gatacgtcga tccagtccga ggtgctggcg accccggttc ccaacgcatt cgcgctgccc
    23521 ggcggcaggg tgtacctgtt cagcgggctg ctggccaagg ccgaaaacgc cgatgaaatc
    23581 gccggcatcc tggcccatga gcttggtcat ctcagccatc gcgatggcac gcgcaatctg
    23641 atctacaatg gcggaacgtc gtttctgatc gggttgctgt tcggcgacat caccggctcc
    23701 ggcgccttga tttttgcttc gcgctcgctg gtgaccgcgt cctattcgcg cgaggcggag
    23761 caaaatgccg atacgtttgc aatcgatgcg atgcacaggc ttggccgctc gtcaaagccg
    23821 atgggcgaat tgatgtttcg catcaccggg aaggaaggcg acaagagcct gtccgtcctg
    23881 tcgaaccatc cgctgacgga ggaccgactg gcgcggatga gccaagggga tcggccgccg
    23941 agcggtccgc cgctgctgac gcagtcggaa tgggcgtcgc tcaaggcaat ctgcagctcc
    24001 gattccaaaa tctgatcatt cagccagatt attgaccttc cgcacccaga cgcggacgtc
    24061 ggccagcacc tcgggcagca ccgccttgac ctccgcgacg gtcatgccgg ctttgacgcg
    24121 tggatcgtac agaatattga ggatgtactg gtcatagacg tcgaaaaacc ccatcgacac
    24181 attgtcgttg aacatggtcc agggcaccga agaggtatcg ttgattggcc ccagcgattg
    24241 cagcagttcc tcatacccgc agtcgaagaa gatgaaatcg ccgttatcga ccgtgaggat
    24301 cacgtcggag tgctcgattt caaagttttc gttcttgcgg aatccggaca ggcattgcgg
    24361 atcgagcgag gtgcgaatct cgcgcgcccg ttcgctgccg tagaacgtcg tgatggtgcg
    24421 atagagatcg cggtcgcgca ccagcttgac cagaacattg gcgccgtcgc tggtttccgc
    24481 catggcgatg tcgagatgct gaatccgctc ggcgatgtcg gccacgatct tcgccagttg
    24541 cgcctttcga tccggacggt tgccctcggc gaacacccgc accggcgcat cgtattttct
    24601 gatgcggtcg acccggccgg ccaggtgata ctcggcgccg aaggcggtct tgaaaaatcc
    24661 gtcggtgatc tcgctgtcgg tgaatgtctt cttttcagcg cgctgacgcg atgcaatcgc
    24721 cggaatttca gtcgccgcaa ctggcggcca ggcaccgccc tcgagcgcga tgccggccaa
    24781 agcgaccgcg aatatccgca acagcaaggc tcgatagttt acgctcatcg cgccgacgct
    24841 gccgcaaccg tggccccgac gcaatcgctg aatgcaggct gcgcatcggg ttccacgtcg
    24901 cgctgcggag cgcgcgggtt cccttgaccg gcgcccgtct agtttttgac gacgaccgtc
    24961 gttccaaccg agacgcggtc atagagatcg gccacgtcgt cattggtcat gcggaagcag
    25021 cccgacgata ccgcctggcc gatcgtctcc ggctcgttgg aaccgtgaat gcgataaagc
    25081 gtcgatccga gatacatggc gcgggcgccg agcgggttgt cgatgccgcc cgccatgtgc
    25141 ctcggcagat cgggccgccg cgccagcatc tgcgagggcg gcgtccagct cggccattcc
    25201 ttcttggcgg atatgcggtg gacgccgccc cagcgaaagc catcgcggcc gacgccaatg
    25261 ccgtaccgca gcgcctggcc gttgggcagc acgaggtaaa gccgccgctc ggcggtatct
    25321 atgagtatgg taccaggcgt gtatcggctt tcaaatctca ccgtctgccg cggaatcggg
    25381 ctggaggcgc cgatgctgaa attggggccg ccgcccatga tgtcgcggct gtcggcgccg
    25441 aagaaaccct gagcgtgcgc gcggtcggcg cccgccagca atgtcagcgc cgcaaacaac
    25501 aaagcaaagg tccgggccat atacttcctc gcgagaaatc agtttgagtg cgagtgaggc
    25561 cataattggc gaaatttcgc aagcgacgag cccgtcaggg ccgcgatttt cagccgttgc
    25621 ggcaaaaatg gcaccgcgcg gcgatagcag caatgcccgt gaagcctttg acgaaaggcg
    25681 tcaggaatga ttgatcttgc ggggagcctc aataagcgtc gcgaacggga gtggaataat
    25741 gaacggtgcg gaaagtctgg tgcggacctt ggtcgcgggc ggcgttgacg tctgcttcgc
    25801 caatcccggc acctccgaaa tgcatttcgt cgcagccctt gatcgggtgg agggcatgcg
    25861 ctgcgtgctc ggcctgttcg agggtgtggt gacgggtgcg gcggacggct atttccgcat
    25921 gaaggggacg ccggcatcga cgctgctgca cctcggaccc ggtctggcca acggccttgc
    25981 caatctgcac aac
//
LOCUS       DATNOI010000028         2861 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2664008,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000028 DATNOI010000000
VERSION     DATNOI010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2664008"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VN968_01030"
     CDS             complement(<1..1101)
                     /locus_tag="VN968_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HXL27864.1"
                     /translation="MRLIRCLAPLALGLMIVVAAAPARAIDAVSVRGDAPAIDLTAVL
                     DHQHSDTDRIQVSTAPGTDGIVRRIEVRAREGGQFWVVFALANNTDDQLDRLIVAPHY
                     RIVSSGLLWPDLGLSRIATITPSTGDRPDRQESATADIFRITLDPGAVITFVAELRTD
                     KLPQLYLWEPDAYKDKVNSFTLYQGIVIGISGLLALVLTILFVVKGSVMFPAAAALAW
                     AVLVYIGVDFGFWGKVLDMSNNAERIWRAAGEAVLAATLLVFLFAYLNLSRWHVRYSH
                     ITVGWLVFLGSLVALALFDPPVASGIARMSLVLIAFAGFALIVYLSTHGFDRAVLLIP
                     TWFLLVVWVIAAGMTVGGFVTNDIVGPALLGGL"
     gene            complement(1238..2236)
                     /locus_tag="VN968_01035"
     CDS             complement(1238..2236)
                     /locus_tag="VN968_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HXL27865.1"
                     /translation="MEKLPAQMTVIGISKPGGPEVLLPETRSVPVPGPGEILVKVMAA
                     GVNRPDVAQRSGAYPPPPGASDLPGLEIAGEVVALGAGAAKHKLGDKVMSLVAGGGYA
                     QYCIAQDAQAMAVPPALSMQEAGAIPETLMTVWHNVFERGALKPGETLLIHGGSSGIG
                     TMAIQLAKAFGAKVLVTVGSKDKADACVRLGADRAINYKTEDFVTEVKTATDGAGANV
                     ILDMVGGDYIERNYDAAATDGRVVQIAFLGGPKANVNFAKLMVKRLHHTGSTLRPRSN
                     TDKAAMVAAIEAKVMPLLREGRVKPVMDSTFPLEKAADAHRRMETSEHIGKIVLVV"
     gene            2339..2530
                     /locus_tag="VN968_01040"
     CDS             2339..2530
                     /locus_tag="VN968_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HXL27866.1"
                     /translation="MAIEDDDKPRKKITHEIGQDLSLLSVEELTERIALLNSEIERLQ
                     QAAAKKRASKDAANSFFKS"
BASE COUNT          579 a          874 c          912 g          496 t
ORIGIN      
        1 gaggccgccg agcagcgccg gaccgacaat gtcattggtg acgaagccac cgaccgtcat
       61 gccggccgcg atcacccaga ccaccagcag gaaccaggtc gggatcagca ataccgcgcg
      121 gtcgaagccg tgggtcgaga gatagacgat gagggcaaaa ccggcgaacg cgatcagcac
      181 cagcgacatc cgcgcgatcc ccgaggcgac cggcggatcg aacaaggcca gcgccaccag
      241 cgagccgaga aagaccagcc agccgacggt gatatgggaa taacgcacat gccagcggct
      301 gagattgaga taagcaaaca ggaacaccag tagcgtcgcc gccaggaccg cttcacccgc
      361 cgcgcgccag atccgctcgg cattgttcga catgtcgagc accttgcccc agaagccgaa
      421 atcgacgccg atatagacca gcaccgccca cgccaatgcg gcggcggcgg gaaacatgac
      481 gctgcccttg accacgaaca ggatggtgag caccagcgcc agcaatccgg aaatgccgat
      541 cacgatgccc tgatacagcg tgaatgagtt gaccttgtcc ttgtaggcgt cgggctccca
      601 cagatagagc tgcggcagct tgtcggttct aagctcggcg acaaaggtga tgacggcgcc
      661 gggatcgagc gtgatgcgga agatgtccgc ggtggcgctt tcctggcggt cgggacggtc
      721 gccggtcgag ggcgtgatgg tcgcgatgcg cgacaatcca aggtccggcc acaacagacc
      781 cgacgatacg atgcgatagt gcggagcgac gatcagacga tcgagctggt cgtcggtgtt
      841 gttggcgagc gcaaacacca cccagaactg accgccttcg cgggcgcgga cttcgatgcg
      901 gcggacgatg ccgtcagtac ccggtgcggt ggaaacctga atgcggtcgg tgtcgctgtg
      961 ctgatgatca agcaccgcgg tgaggtcgat cgcgggcgcg tcaccgcgga cgctgacggc
     1021 gtcgatggca cgcgctggcg ccgcggcgac aacaatcatg aggcccagcg cgaggggcgc
     1081 gaggcacctg atcagacgca atgtcagttc tccgcgttcg acaatgtgaa gatgcaagac
     1141 ccgtttcgaa cgcggcgagc gcgttcggca tcctgcgata aatggcatga aagcgaggga
     1201 gatcaaagag tttccggctg gccgccattc acgagcgcta aacaaccaac acaattttgc
     1261 caatatgttc gctggtctcc atgcggcggt gcgcgtctgc ggctttttcc agcgggaatg
     1321 tgctgtccat gacgggtttg acccgcccct cgcgcaacag gggcatcacc ttggcctcga
     1381 tggcggcgac catcgccgcc ttgtccgtat tgctacgggg gcgcagcgtg gagccggtgt
     1441 ggtgcagcct tttcaccatc agcttggcga aattgacgtt tgccttcggc ccgccgagaa
     1501 atgcgatctg gaccacgcgg ccatcggtcg cagctgcgtc gtaattgcgc tcgatgtaat
     1561 cgccgccgac catgtcgaga atgacgttgg cgcccgcgcc atcggtcgcc gtctttacct
     1621 cggtgacgaa atcctcggtc ttgtaattga tcgcgcggtc ggcgccgagc ctgacgcagg
     1681 catcggcctt gtcctttgat ccgacggtga cgagcacttt tgcgccaaaa gccttggcca
     1741 attggatcgc catggtgccg atgccggatg aaccgccgtg gatcaggagc gtctcgcccg
     1801 gtttcagcgc gccgcgctcg aacacattgt gccagaccgt catcagcgtt tccgggatgg
     1861 cgccggcctc ttgcatcgac agcgccggtg gcaccgccat cgcctgagca tcctgggcga
     1921 tgcaatattg cgcgtagccg ccgccggcca ccagcgacat caccttgtcg ccgagcttgt
     1981 gcttggccgc gccagcgcca agcgcaacca cttcgccggc gatttcaagg ccgggcaggt
     2041 cgctggcgcc gggcggcggc gggtaggcgc cggagcgctg cgcgacatcc gggcggttga
     2101 cgccggcggc catcaccttg accaggattt cgcccggacc ggggacggga acgctgcggg
     2161 tttcaggcaa cagcacttcg gggccgccgg gcttgctgat gccgatgacg gtcatttgcg
     2221 cgggcagctt ttccatgatt tgtccttgcg aaatgcggga ttgaaggcgg cccctgatta
     2281 gccagcccgg ctcaagctgg caaccgcttc gggaaccgcc gagagcaagg agaaacgcat
     2341 ggccatcgag gatgacgaca agccgcggaa gaaaatcacc cacgagatcg gccaggacct
     2401 gtcgctgctg tcggtcgagg aattgaccga gcgcatcgcg ctcttgaatt cggaaatcga
     2461 acggctgcaa caggcggcgg ccaaaaagcg cgcgtcgaag gatgccgcga acagtttttt
     2521 caagtcgtga tctttgagtg cagaaccgta gcccggatgg agcgcagcgc aatccgggga
     2581 tgccacatca atgcgcgata gggccccggg ttgcgctgcg ctccacccgg gctacacgaa
     2641 cgagaacgca agtcgaaaac tcggccagtg gccgacatgg caaacgatcc ctaaagaaaa
     2701 tcgctcgttt acgatcaatt aagctttctc gtttatgact gggatcgtcc tcgtttggac
     2761 accgagtggc tcctgtccac tctgtttgac gcctccctgt tatcaacttt aaaagccgcc
     2821 ggaaacggcg gctctttttt tggcgtccgt ggtacccggc t
//
LOCUS       DATNOI010000029         2558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2662517,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000029 DATNOI010000000
VERSION     DATNOI010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2662517"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VN968_01045"
     CDS             complement(<1..216)
                     /locus_tag="VN968_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HXL27867.1"
                     /translation="MVRRQVPIISQSCCERCDVATRDGRSLGCIQSLSCHTDRCPTGV
                     TTQDPTRARALVVPHKIERVYNYHHATL"
     gene            complement(192..989)
                     /locus_tag="VN968_01050"
     CDS             complement(192..989)
                     /locus_tag="VN968_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HXL27868.1"
                     /translation="MPKRTLQICLLWTYAILGAVDAQADSCPTAKDEIATDRPDVTNS
                     SLVVPMGSLQSENGVNFSARDGGRFVDGTNTRWRLGIAPCLEILLDLPTYVADIRAPG
                     SSGFSDVAPAIKWQVSPIPGKVDLSLVFGVALPTGAADIAGRGAQPYLQMPWSWELRD
                     GWGLSGMLTEFFRPSELTSRRITETTFVIERKVTEKASLFVEYVGDYPESASPSQLLN
                     SGGVYRLNPTQQVDFHVAFGLNHNAPSYIVGVGYSFRLDGLFAGKFP"
     gene            complement(1124..>2558)
                     /locus_tag="VN968_01055"
     CDS             complement(1124..>2558)
                     /locus_tag="VN968_01055"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HXL27869.1"
                     /translation="LRNYPIAAHIRFLLEEIRPEMRQYFFESEKDGMPFSRDTRALVY
                     QRAKMVLDKRPFGTQEDVYRDGYEWMHHSVAPKARAVEQFRVTIGGPDCTKPYSASVF
                     NISAMSFGALSPNAVRALNAGARKGGFAHDTGEGGVSPYHRENGGDLIWEIGSGYFGC
                     RNRDGSFNPEEFARVATDDQIKMVELKISQGAKPGHGGVLPAAKVSEEISKIRGVAMG
                     EDCISPATHRAFSTPLEMMAFIGEMRRLSGGKPAGFKLCIGHPWEFLAICKAMLQTGI
                     YPDFIVVDGNEGGTGAAPLEFMDHLGMPMREGVNFVHNALIGINARDRIRIGAAGKIA
                     TAFDMARAMAIGADWCNSARGFMFSLGCIQSLSCHTDRCPTGVTTQDPTRARALVVPH
                     KIERVYNYHHATLQALAELLAAAGLEHPQELRPIHFSKRSSTTDVLSFAKLYPSLRPG
                     ELIDGTSDPRFREAWAMARADSFAPAG"
BASE COUNT          520 a          791 c          766 g          481 t
ORIGIN      
        1 cagggtagcg tgatgataat tgtagacccg ctcgatcttg tgcggcacca ccagggcgcg
       61 agcccgggtt ggatcctgcg tggtcacgcc ggtcgggcac cgatcggtat ggcaggagag
      121 cgactggatg cagcccagcg atcggccgtc acgagttgcg acgtcacacc gttcgcagca
      181 cgactggcta attatgggaa cttgcctgcg aacaatccat caaggcggaa tgaatacccg
      241 accccgacga tatagcttgg tgcattgtga ttgagcccga aggcaacatg gaaatcgacc
      301 tgttgagtcg ggtttaggcg atacactccg cctgaattga ggagctggct cgggctcgca
      361 ctctcgggat aatcaccaac atattccaca aacaagctcg ccttctcggt caccttcctt
      421 tcgatcacaa aggtagtctc cgtgatgcgt ctgcttgtca gctcggacgg acggaagaac
      481 tcagtcaaca tcccgctcag gccccaacca tcgcgcaatt cccaggacca tggcatttgg
      541 agatagggct gcgcgccgcg gcccgctata tcggccgcac cggtcgggag tgccacgccg
      601 aacacaagag acaaatcaac cttgcccggg atcggactga cctgccactt tatcgccgga
      661 gcaacatccg aaaaccccga gcttcccggg gctcggatgt ctgcaacgta ggtcggcaga
      721 tcaagcagga tctccagaca tggcgcaatt ccgaggcgcc atcgggtgtt tgtgccatcg
      781 acaaatcggc cgccgtcacg ggcactgaaa ttcaccccgt tctcactctg aagactgccc
      841 atgggcacga cgaggctgga gttcgtgaca tccggccgat ccgtcgcgat ttcgtctttg
      901 gcggttggac acgagtcagc ctgggcgtcg accgcgccga ggatcgcgta tgtccacagc
      961 aaacatatct gaagagttcg tttcggcacg gcagactaaa tgaagttttg ttttcggata
     1021 tggacctgac gggcgctcgg ctacaaaagc agaacaacct acattgccga gaattccgtc
     1081 taatctgtaa aaatgctgcc gcgccccgga atgacggcgc gcctcacccc gccggcgcaa
     1141 aactgtcggc ccgcgccatc gcccaggcct cacgaaagcg cggatcgctg gtgccgtcga
     1201 tcagttcgcc cgggcgaagc gacggataaa gcttcgcgaa tgacagcaca tcagtggtgg
     1261 aggatcgctt cgagaaatgg atcggccgca attcttgcgg atgttcgagg cccgcggcgg
     1321 cgagaagctc ggccagcgcc tgcagggtgg cgtgatggta attgtagacc cgctcgatct
     1381 tgtgcggcac caccagcgcg cgagcccggg ttggatcctg cgtggtcacg ccggtcggac
     1441 accgatcggt atggcaggag agcgactgga tgcagcccag cgaaaacatg aagccgcgcg
     1501 ccgaattgca ccagtcggcg ccgatcgcca tggcgcgcgc catgtcgaag gcggtagcga
     1561 tctttccggc ggcgccgata cggatgcggt cgcgcgcatt gatgccgatc agcgcgttat
     1621 ggacgaaatt gacgccctcg cgcatcggca tgccgagatg gtccatgaat tccagcggcg
     1681 cggcaccggt gccgccttcg ttgccgtcga ccacgatgaa atcgggatag attccggtct
     1741 gcagcatcgc cttgcagatc gccagaaatt cccaggggtg gccgatgcaa agcttgaatc
     1801 cggcgggctt gccgccggac agccgccgca tttcgccgat gaaggccatc atttccagcg
     1861 gcgtcgaaaa tgcccggtgg gtcgctggcg aaatgcagtc ctcgcccatc gcgacgcctc
     1921 tgatcttgga aatctcctcg gataccttgg cggcaggaag cacgccgccg tgcccgggct
     1981 tggcgccctg gctgatcttg agttcgacca tcttgatctg atcgtcggtc gcgacgcgcg
     2041 cgaattcctc cggattgaac gagccgtcgc ggttgcggca accgaaatat ccggagccaa
     2101 tctcccaaat gagatcgccg ccgttctcgc gatgataggg actgacaccg ccctcgccgg
     2161 tgtcatgcgc gaagccgcct ttcctggcac ccgcgttcag cgcgcgcacc gcgttgggac
     2221 taagcgcgcc aaagctcatc gccgagatat tgaacaccga cgccgaatac ggcttggtgc
     2281 aatcgggacc gccgatggtg acacgaaatt gctcgaccgc acgagccttc ggcgccaccg
     2341 aatggtgcat ccattcataa ccgtcgcgat agacatcctc ctgggtgccg aacggccgct
     2401 tgtcgagcac catcttggcc cgctgataga cgagcgcgcg ggtgtcgcgg ctgaacggca
     2461 tgccgtcctt ttcgctctcg aagaaatact gccgcatctc cggcctgatc tcttccagca
     2521 ggaaccgtat gtgcgccgct atcggatagt ttcgcagc
//
LOCUS       DATNOI010000030         5674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2661468,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000030 DATNOI010000000
VERSION     DATNOI010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2661468"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VN968_01060"
     CDS             complement(<1..675)
                     /locus_tag="VN968_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27870.1"
                     /translation="MACHDGVAVVEFHPLLGHPIHMSLTLIIVAAIAVIWFAERSVEH
                     LKLAIAALCFNAAVLLFVVTDFERAILLSSILAAAIFGASNVKYNHSGLKLTVTDLPL
                     AFAGTVPFFIVQYPLATIAVLAGGIALILAAVAALLCMAGPPVSLGIQALLFGVAFIS
                     LVATYRASGGAVSFQRIAALRRCFFSTFIASLLDPLSWRQFGKLALSDIAKDPLPLMA
                     AVPARTL"
     gene            complement(906..2978)
                     /locus_tag="VN968_01065"
     CDS             complement(906..2978)
                     /locus_tag="VN968_01065"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HXL27871.1"
                     /translation="MPAASYLDTLNVQQRLAVEHGVRERDCAPRSPLLVIAGAGSGKT
                     NTLAHRVAHLIVNGADPRRILMMTFSRRAASEMTRRVERIARKVMGDGACVMTDALTW
                     AGTFHGIGARILRDYADQIGLDPAFTIHDREDSADLINLARHELGFSKTQNRFPTKGT
                     CLAIYSRCVNAESGIEQVLGKSFPWCSGWAAELKQLFAAYVEAKQRQNILDYDDLLLY
                     WAQTVGDAKLADDIGGRFDHVLVDEYQDTNRLQSSILLALKPDGRGLTVVGDDAQSIY
                     SFRAATVRNILDFPGQFTPNATIVTLDRNYRSTATILAAANGVIELASERFTKNLWTE
                     RTASTKPQLVSVRDEADQARYIVERILESRESGIVLKQQAVLFRTSHHSGPLEVELTR
                     RNIPFVKFGGLKFLDAAHIKDMLAMLRFVENPRDRVAGFRLMHLVPGVGSTSAQRVLD
                     HMAKASDPFVALAEAPSPPRAGDDWQSFIATVTDLGSGRAGWPAELERARLWYEPHLD
                     RIHEDAGTRRADLVQLEQIAGGYPSRERFLTELTLDPPDATSDQAGVPLLDEDYLILS
                     TIHSAKGQEWKSVFVLNVVDGCMPSDLGAGTSAEIEEERRLLYVAMTRAKDDLHLVVP
                     QRFFTHGQSAQGDRHVYASRTRFIPDRLLGLFERTGWPLAETGTAARAANKSVRLDVG
                     ARMRGMWR"
     gene            complement(3076..3825)
                     /locus_tag="VN968_01070"
     CDS             complement(3076..3825)
                     /locus_tag="VN968_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HXL27872.1"
                     /translation="MRFLIMILVFFAAGSACAEDALVKDGATVQLAGVTFKLDGIDAP
                     EFDQMCIDEHAEPWACGVEARDQLAKLIDRRGVHCEDLGPSRTYGKWHIGLCTVDGET
                     TSLNALLVRQGFALNFEPDAKGRFKQDEANAKDKLSGLWRGCFVAPQDFRHDEKSGAL
                     LGSACRSDKDRELREALFPDEPAMPPGCSIKAKFARRARLTGYVGVYHLQVCRSYAPV
                     TKPDRWFCSEEDAQAAGFRKAYNCRAIVRRK"
     gene            complement(4093..5550)
                     /gene="hydA"
                     /locus_tag="VN968_01075"
     CDS             complement(4093..5550)
                     /gene="hydA"
                     /locus_tag="VN968_01075"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HXL27873.1"
                     /translation="MAPLIIRGGTVVNHDHSRRADIVVDGETIVAVGAAVDAPAGAEI
                     IDAGGCYVMPGGIDPHTHLEMPFMGTVTADDFEWGTKAALAGGTTMVVDFCLPDPGQS
                     MLAAYQDWRRKSERAASDYGFHMAVTSWSKQVFDEMEIVVKTYGINTFKHFMAYKGAL
                     MVNDDELYNSFARCAHLGAMPLVHAENGDVVARMQEALLERGVTGPEGHAYSRPPEVE
                     AEATNRAIMIADMTGAPLYVVHTSCREAHEAIARARAHGKRVYGEPLIQHLLLDATEY
                     ENKSWNHAARRVMSPPFRARVHQDSLWAGLQSGSLQVVATDHCAFTTEQKRLGFGDFR
                     KIPNGTGGLEDRLPLLWTAGVNTGRLTKEEFVAVTSSNIARILNIYPRKGRLSAGSDA
                     DIVVWDPAASKNITAKSQLSRIDYNVFEGFACLGAPVVTLSRGKIAFKEGELRAEKGD
                     GRYIERPPFPPVHVANSTWKLQNAPQAVRRSDVTP"
BASE COUNT         1105 a         1702 c         1798 g         1069 t
ORIGIN      
        1 gagggtgcgt gctggtacgg ccgccatcag cggcaagggg tctttagcta tatcgctcag
       61 agcgagcttg ccgaactgtc gccaggaaag cgggtcgaga agggaggcta tgaaggtcga
      121 aaaaaaacac cgccgcagag ccgcgatccg ctggaaagaa acggccccgc cgcttgccct
      181 gtatgttgct accaaactga taaatgcaac gccgaataaa agagcttgga ttcccaaaga
      241 aactggcggc ccggccatgc agagcagagc cgcgaccgcg gccaggatga gtgcgatgcc
      301 tccagcgaga actgctattg ttgcgagcgg gtattgcaca ataaaaaatg gaactgttcc
      361 ggcaaacgct agcggcaaat ccgtcaccgt cagtttcagc ccgctatgat tatacttgac
      421 atttgacgct ccgaaaattg ccgctgccag gatagacgat agcagtatag ctctttcgaa
      481 atcggtaacg acaaacagca gcaccgcggc attgaaacac agcgctgcaa tagccaattt
      541 aaggtgctca acgctgcgct ctgcaaacca aatgacggcg atggctgcca caattatcag
      601 ggtcaatgac atatggatcg gatggccaag tagcggatga aattctacga ctgctacacc
      661 atcatggcag gccaagacag ctgctgttca attgtcttga tgacgatcaa gacccgcgat
      721 cctaacgtcc gcgatcctaa cttcaatgtc gcttttcggc ccatcgcgtc gtttcgctgc
      781 gatgcaacga ttcgtcggct atcagggaca aggcgggctc gggcagtcag tccgatcgat
      841 gcatcaatga tttcgcggcc tgatgaatcg gcgcgtacag agctggaacg aggcaacttc
      901 gaggctcacc gccacatccc gcgcatgcgg gcgccgacgt ccagcctaac acttttgttc
      961 gccgcgcggg ccgcagttcc ggtttctgca agcggccagc ctgtcctttc gaacaggccg
     1021 agcagccgat ccggaatgaa ccgcgtccgt gaggcataga cgtggcgatc gccttgcgcg
     1081 ctctggccgt gggtgaagaa acgttgcggc acgacgaggt gcagatcgtc cttggcgcgg
     1141 gtcatcgcca catacagaag ccggcgctct tcctcgattt cggccgaggt gccggcgccg
     1201 agatccgacg gcatgcatcc atcgacgaca ttgaggacga acaccgactt ccattcctgg
     1261 cccttggcgg aatggatcgt ggagaggatc agatagtcct cgtcgagcag cggcacgccg
     1321 gcctggtcgc tggtcgcatc gggagggtcg agcgtgagtt cggtcaggaa acgctcccgt
     1381 gaaggataac cgccggcgat ctgctcgagc tgaacgagat cggcgcgcct tgtgccggcg
     1441 tcctcgtgaa tgcgatcgag atgcggttcg taccagagcc gggcacgttc cagctcggcc
     1501 ggccagccgg cgcggcccga gccgaggtcg gtgacggtcg cgatgaagct ttgccagtcg
     1561 tcgccggcgc ggggaggcga cggggcttcg gcgagagcga caaagggatc gctggccttg
     1621 gccatgtggt cgagcacgcg ctgcgccgat gtggacccga cgccgggaac aagatgcatc
     1681 agccgaaacc cggcgacacg gtcgcgcggg ttctcgacaa agcggagcat cgccagcatg
     1741 tccttgatat gcgcggcatc gagaaatttt aggccgccga acttgacgaa gggaatgttg
     1801 cgccgggtta gctcgacctc cagcgggccg ctgtgatgcg aggtgcggaa cagcacggcc
     1861 tgttgcttga ggactatccc ggactcgcgg ctctcgagga ttcgctcgac gatgtagcgg
     1921 gcctggtcgg cctcatcgcg caccgacacc agctgcggtt tcgtggaggc ggtccgctcg
     1981 gtccacaggt tcttggtaaa acgctcgctg gcgagttcga tgacgccgtt ggcggcggcg
     2041 agaatggtcg ctgtcgagcg gtagttgcga tccagcgtga cgatcgtggc atttggggtg
     2101 aactggccgg ggaagtcgag aatgttgcgc accgttgccg cacgaaacga atagatcgac
     2161 tgcgcgtcgt cgccgaccac ggtgaggcct cggccatccg gcttcagcgc cagcaggatc
     2221 gaggactgca ggcggttggt gtcctggtat tcgtcgacca ggacatggtc gaagcggccg
     2281 ccgatgtcgt ccgccaattt ggcatcgccg acggtctggg cccagtacag caggaggtcg
     2341 tcgtaatcga gaatgttttg ccgctgtttg gcttcgacat aggcggcgaa caattgcttg
     2401 agctcggccg cccatcccga acaccaggga aacgattttc cgagcacctg ttcaatgccg
     2461 gactccgcat tgacacatcg ggagtagatc gcaaggcagg tgcccttggt cggaaagcgg
     2521 ttttgggttt tggagaagcc gagttcgtgc cgcgccagat tgatcaggtc ggccgaatcc
     2581 tcgcggtcat ggatcgtgaa cgccgggtcg agaccgatct ggtcggcata gtcgcgcaga
     2641 attctggctc cgatgccgtg aaacgtaccg gcccaggtca gcgcgtcggt catgacgcag
     2701 gctccgtcgc ccatcacctt gcgggcaatc cgctcgacgc ggcgggtcat ctcgctggcc
     2761 gcacggcgcg agaatgtcat catcagaatg cggcgcggat cagcgccgtt gacgatcaga
     2821 tgggcgaccc gatgggccag cgtattggtc ttgccggatc ccgcgccggc aatgaccagg
     2881 agcggcgatc gcggcgcgca atcgcgttcc cgcacgccgt gctcgacggc aagccgctgc
     2941 tgcacgttga gggtgtcgag gtagctcgca gcgggcatga ttgattcgtc cgagggagaa
     3001 cgacgctcgc tgattcctgc tttgttcgca atggccgact ggatcgaggt gccggttccc
     3061 cggcattcgc ggcgcttact tgcgacgaac aatggccctg caattatacg ccttgcgaaa
     3121 cccggcggcc tgggcgtctt cctcggagca gaaccagcga tccggttttg tcacgggggc
     3181 gtaactccgg cacacctgca ggtgatagac accgacgtaa cctgtcaggc gcgcgcgcct
     3241 agcgaatttt gccttgatgc tgcagccggg cggcatcgcc ggttcgtccg gaaacagggc
     3301 ctctcggagc tcgcggtctt tgtcgcttcg gcaggccgag ccgagcagag cgcctgactt
     3361 ttcgtcatgg cggaaatcct gcggggcgac aaagcagcct cgccagaggc cgctcagctt
     3421 gtcctttgcg ttcgcctcgt cctgcttgaa ccggcccttg gcgtcgggct caaaattcag
     3481 cgcaaaaccc tgacgcacca gcaacgcgtt cagactggtg gtttcgccgt cgacggtgca
     3541 aagcccgata tgccatttgc cgtaggttct gctcggcccc agatcctcgc agtggacgcc
     3601 gcgcctatca atcagtttcg caagctggtc gcgcgcttcc acgccgcacg cccagggttc
     3661 ggcatgttcg tcgatgcaca tctggtcgaa ctccggagca tcgatgccat cgagtttgaa
     3721 agtcacgccg gcgagctgga cggtggctcc gtccttgacg agcgcgtctt cggcgcaagc
     3781 cgaacctgct gcgaaaaata ccaggatcat gatgagaaaa cgcatcgact taaaccagcc
     3841 cgccaaaatg ataatttcgc ccgaccttat cgcacaacag gattggagaa cgagctggaa
     3901 acagcggatt tcaagaatta gaacagtttt cgacaatttg caccggcagg caaccgatga
     3961 agcagccgcg atcatgctgg ctcagctctc gtcggcacat ggtctggcgg cttcggggcg
     4021 aagcggccga agcgctctcc acgacgaatg tcttccgact cgagaccacc ggcgttccat
     4081 cgaaacggga gtctagggcg ttacgtcgga acgccgcacc gcttgcggcg cgttctgcaa
     4141 tttccaggtc gaattggcga catggaccgg cgggaacggc ggccgctcga tgtaacgtcc
     4201 gtcgcccttc tcggcgcgaa gctcgccttc tttgaaagcg atcttgccgc gcgacagcgt
     4261 gacaaccggc gcgcccaggc aggcaaagcc ttcgaacaca ttgtaatcga tgcggctcaa
     4321 ttggctcttg gcggtgatgt tcttgctcgc cgccggatcc cacaccacga tgtcggcgtc
     4381 cgagccggcg gaaagccggc cttttctcgg atagatattg aggatgcggg cgatgttcga
     4441 agacgtcacc gcgacgaatt cttccttggt cagccggccg gtgttgacgc cggcggtcca
     4501 gagcagcggc aggcggtctt cgagcccgcc ggtgccgttc gggatcttgc ggaaatcgcc
     4561 aaagccgaga cgcttctgct cggtggtgaa cgcgcagtgg tcggtcgcga ccacctgcag
     4621 cgagccggat tgcaggccgg cccaaagact gtcctgatgc actctggcgc ggaacggcgg
     4681 cgacatcacg cgccttgcgg catgattcca gctcttgttt tcatattcgg tagcatcgag
     4741 cagcagatgc tggatcagtg gctcgccgta gacgcgcttg ccatgggcgc gggcccgcgc
     4801 aatcgcctca tgcgcctcgc ggcagctggt atgcacgaca tagagcggcg cgcccgtcat
     4861 gtcggcgatc atgatggccc ggttggtcgc ctcggcctcg acctccggcg ggcgcgagta
     4921 ggcgtggccc tcggggcccg tgacgccgcg ctccagcagg gcttcctgca tgcgcgcgac
     4981 cacgtcaccg ttctcggcgt gaaccagcgg catggcgccg agatgggcgc aacgcgcaaa
     5041 cgagttgtaa agctcgtcgt cattgaccat cagcgcgccc ttgtaggcca tgaagtgctt
     5101 gaaggtgttg atgccgtagg tcttgacgac gatctccatc tcgtcgaaca cctgtttcga
     5161 ccacgaggtc acggccatgt ggaagccgta gtcgctcgcg gccctctcgg atttgcgccg
     5221 ccagtcctga tacgccgcca gcatcgattg cccgggatcc ggaaggcaga aatccacgac
     5281 catcgtggtg ccgccggcca gcgccgcctt ggtgccccac tcgaaatcgt cggcggtgac
     5341 ggtacccatg aacggcattt cgagatgggt gtgcggatcg atgccgcccg gcatcacgta
     5401 acaaccgccg gcgtcgataa tttcggcgcc ggcgggggcg tcgacggcgg cgccgaccgc
     5461 gacgattgtc tcgccatcga caacaatgtc ggcgcgccgc gaatgatcat ggtttacgac
     5521 cgtaccgccg cgaatgatga gaggggccat ggaaactccg aacaggcaat tcgaaaagat
     5581 ggtatcgcag cgtccgcggc gacctttccc ggcagcctag cttgcaattc tcgcggaagc
     5641 agcgaaaatt ttgccagatt gcgccgtgca gtcg
//
LOCUS       DATNOI010000031         7365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_707319,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000031 DATNOI010000000
VERSION     DATNOI010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707319"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VN968_01080"
     CDS             <1..822
                     /locus_tag="VN968_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXL27874.1"
                     /translation="ETTIDTGTTELLCVIRDRVAILTLNRPEVRNALSDHLTPALRTM
                     IKTCGESPDVGALLITGAGTAFCAGGNVKGMGAHRDQKKLEMSYDEKVADLQERQRLL
                     TGALVSVRKPTIAALPGPAVGAGLAIAMACDIRIAAESAFISTGYLRVGLSGDYGIAW
                     LLTRLVGTSRARELMFTADKVDAARCEAIGLVNRVVPDAKLQDEAFALAKSMAEGPTI
                     ALRYMKDNLDEALSFDFATARDHEAERLIRTTMTSDHREAVQAFIEKRKAVFRGS"
     gene            complement(827..1441)
                     /locus_tag="VN968_01085"
     CDS             complement(827..1441)
                     /locus_tag="VN968_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL27875.1"
                     /translation="MIALRFNDLRQHSDVLRSRSGKPLTVRFVEPRDADALQGYFRSL
                     SVGSRYNRFLGAMSELPQSQLDHFVHVGEAGRFSVIALMTIDGVETIVGEGRYAFDAE
                     TSSFEFGLSIDDRWQGQGIGSALLKNLQCRAAAFGAERLFGDTLRSNDVMISLARKSG
                     FAFTNSPGDWKLVRFEKHIDAAPQDIPCASWRLAAQQIALQAAV"
     gene            complement(1513..1716)
                     /locus_tag="VN968_01090"
     CDS             complement(1513..1716)
                     /locus_tag="VN968_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HXL27876.1"
                     /translation="MSTCTHESMTNHHGSGVFAQIGETLRLWRERQHERRALAELTDR
                     DLHDVGLSRGDIYFEAEKPFWRA"
     gene            1832..2731
                     /locus_tag="VN968_01095"
     CDS             1832..2731
                     /locus_tag="VN968_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HXL27877.1"
                     /translation="MTVRLPSLNGLRAFEAAARHLSFTVAASELNVTQTAISHQIRRL
                     EEELGIRLFIRQNRALALTPEARDYLPGVRAAFNDLRLATDRLLRRDNDHVLTVSTLA
                     SLAAKWLLPRLSAFQEANPGIDVRITTSTALIDFKSGDVDAAIRYGRGQWPGLRAHWL
                     MADELFPVCSPALLAGKKPLRRPEDLAEHVLLHNSAGNNDDWRLWLTAAGLPANLSKQ
                     PGITFDLVYMTVQAAIDGIGVAMGRTSYVEADIAKGRLVVPFKITLPADAGFYLVSPE
                     ARADSPKLAAFRQWLSASVQNKI"
     gene            2886..4205
                     /locus_tag="VN968_01100"
     CDS             2886..4205
                     /locus_tag="VN968_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL27878.1"
                     /translation="MSETSTSEPPQIKSRLGAILRATSGNFLEQFDFFLFGFYASYIA
                     KAFFPSENETAALLNTFGVFWLGALMRPVGAIVLGAYIDRIGRRKGLIVTLAIMALGT
                     VTIAVCPTYATIGVAAPVIVLIGRLLQGFSAGVELGGVSVYLSEIATPGNRGFYTSFQ
                     SASQQVAIFVAAIIGFVLSEMMPAATVAEWGWRIPFFIGCLIIPFIFFLRRTLEETPE
                     FLAMKKHPSTSEVFSSAAVNWKIILLGMMLAAMTTVTFYFVTVYTPTFGKTVLKLSSQ
                     DALLVTLLVAVTNFIWNPVGGALSDRIGRKPVLMAIAGLSFVTAYPALLWLTAAPTFG
                     KMLAVELMFSLYFGVYSGTMLGALVEIVPKHVRTTCFSLAFALAAALFGTFTPFAATT
                     LIAMTSDRASPGYWLMCAAASGFIAAVVIYRSGAIEAREQAVAAQQA"
     gene            4273..5670
                     /gene="tcuA"
                     /locus_tag="VN968_01105"
     CDS             4273..5670
                     /gene="tcuA"
                     /locus_tag="VN968_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HXL27879.1"
                     /translation="MVDFNCKFDVLVIGGGNAALCAAISARRTGASVLVLEGAPKFYR
                     GGNTRHTRNMRCAHDAATDILTGPYTEQEFWEDLLRVTGGETDEELARHMIRESKDIL
                     GWIVEQGVRWQPSLGGTLSLGRTNSFFLGGGRAMLNALYLTAEQLGVEILYDAEVLDL
                     QIDNGMFLSADLKQGDGRVTVRASTLVAAAGGFEANIEWLKEYWGDAADNFLIRGTPY
                     NRGSILKMLLNKGVQEIGDPRQCHAVAIDARAPKFDGGIITRHDSVVFGIVVNKHAER
                     FYDEGEDIWPKRYAIWGRLVAAQPDQIAYIIFDASSRNSFMPTLFPPIEAGSIAKLAG
                     EFELDPAALEKTVGDFNAAVRPGTFDHTILDDCRTKGLAPPKTHWARKIETPPYYAYP
                     VRPGITFTYLGTRVNKQARMLMKDGKPAANMFAAGEIMAGNVLGKGYAAGMGMTIGSV
                     FGRIAGREAAQNARN"
     gene            5729..6772
                     /locus_tag="VN968_01110"
     CDS             5729..6772
                     /locus_tag="VN968_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HXL27880.1"
                     /translation="MSRIPRAMLAAALTVATSAVANAAEPIALRDMGSFHVGGRLVEI
                     SGKPVKEITFTPGGVPAKVDPNGTYQVEQMYVQYFLPANEIGAYPLLLWHGGGLTGVT
                     YETTPDGREGWLNYFLRKGWSVYNSDAVERGRAGWAQYPDIFKSEPVFLTTSNPFERF
                     RIGDGPGSYDPDPAKRKLMPGSQFPNQGYENFVKQNVPRWTTNDDATLAAYIAEIDRV
                     CPCIVLFHSQAGTFGFKAAQARPDKVKALIAVEPAGVGDPARIDALKGIPSLLIYGDF
                     IERDSRWPKIRATGIAFADGIKAAGGSADVVDLPKAGISGNSHMMMMDKNNTEVAALI
                     QTWLQGKGLTKKP"
     gene            6803..>7365
                     /gene="tcuB"
                     /locus_tag="VN968_01115"
     CDS             6803..>7365
                     /gene="tcuB"
                     /locus_tag="VN968_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140813.1"
                     /note="with CitA (TcuA) catalyzes the oxidation of
                     tricarballylate to cis-aconitate; originally thought to be
                     involved in citrate utilization; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization protein TcuB"
                     /protein_id="HXL27881.1"
                     /translation="MHGTKILEEADRLMTVCNSCRYCEGLCAVFPAMEMRRSFSDGDL
                     NYFANLCHACGACYTDCQFSPPHEFNVNVPQTLAVARTDSYAAYAWPRAFSGVFARNG
                     LAISIIAALSVAAFIFGFAALNDRQVLLGIHTGPGAFYALMPHNAMAALFGAAFVYAI
                     LALVMGVRAFWRDIGEPVGMKTDAPSL"
BASE COUNT         1381 a         2316 c         2289 g         1379 t
ORIGIN      
        1 gaaaccacca tcgacaccgg caccaccgaa ttgctctgcg tgatccgcga ccgcgtcgcc
       61 atcctcacgc tcaaccggcc ggaagtccgc aatgcgctgt cggaccatct gacgccggcg
      121 ctgcgcacca tgatcaagac ctgcggcgag agccccgacg tcggcgcgct gctgatcacc
      181 ggcgccggca cggcgttctg cgccggcggc aacgtcaagg gcatgggcgc tcaccgcgac
      241 cagaagaaac tcgaaatgtc ctatgacgag aaggtcgcgg acttgcagga acggcaacgc
      301 ttgctgaccg gcgcgctggt ctcggtgcgc aagccgacca tcgcggcgct gcccggtccc
      361 gcggtcggcg cgggactggc catcgccatg gcctgcgaca tccgcatcgc ggcggaatcg
      421 gccttcattt ccaccggcta tctgcgggtg ggcttaagcg gcgactacgg catcgcctgg
      481 ctgttgacgc gccttgtcgg cacgtcccgg gcgcgagaac tgatgtttac cgccgacaag
      541 gtcgacgccg cgagatgcga ggcgatcggc ctcgtcaacc gcgtggtgcc ggatgcaaag
      601 ctccaggatg aggcgtttgc gctggccaaa tcgatggcgg aagggccgac gattgcgttg
      661 cgctacatga aggacaatct cgacgaagcc ctgtcgttcg atttcgccac cgcccgcgac
      721 catgaggccg agcgcctgat ccgcaccacg atgacgtcgg atcaccgcga agcggtgcag
      781 gcttttatcg agaagcgcaa agcggtgttc agggggagct gagccttcaa accgccgcct
      841 gaagcgcgat ctgttgggcc gccagccgcc agctggcgca gggaatgtcc tgcggcgccg
      901 cgtcgatatg tttttcgaag cgtaccagct tccagtcgcc ggggctgttg gtgaaggcga
      961 aaccggattt gcgggcaagc gagatcatga cgtcgttgga gcgcaaggta tcgccgaaca
     1021 gccgttcggc accaaaggcg gcggcgcggc attgcagatt tttgagcagc gccgaaccga
     1081 tgccttggcc ttgccagcgg tcgtcgatcg acaggccaaa ctcgaaactg gaggtctcgg
     1141 catcgaacgc gtagcggcct tcgccgacga tagtctcgac gccatcgatc gtcatcagcg
     1201 cgatcacgct gaagcggccg gcctcgccga catggacgaa atggtcgagt tgggattgcg
     1261 gcagttcgct catggcgccg aggaagcggt tgtagcgcga gccgaccgag agcgagcgga
     1321 agtaaccctg caatgcgtcc gcatcgcgcg gttcgacaaa gcgcaccgtc aacggttttc
     1381 cgctgcgcga ccgcaagacg tcggaatgct gccggaggtc gttgaaccga agcgcgatca
     1441 tggctggctc catcaatcaa aaaagttgtc ggtcggcatc ccccctaaga ggcgacgccg
     1501 acctggcggc gtttacgccc gccaaaacgg cttttcggct tcgaaataga tgtcgccccg
     1561 cgagaggccg acatcatgca ggtcacggtc ggtcagttca gccagcgcgc gccgttcatg
     1621 ctgacgctcc cgccagagcc gcagcgtttc gccgatttga gcgaaaacgc ctgaaccatg
     1681 atgattcgtc atcgattcgt gtgtacaagt ggacattttc atctcctaaa gctagtctgt
     1741 tgcctccaac gtgtgggtta tccagccttt ccacaaacga cactttgtac ggctttacat
     1801 gacataaact catgtatgga tgatgccgga aatgactgtc cgactgccat cgctcaacgg
     1861 actgcgcgcg ttcgaggccg cggcgcggca tttgagcttc acagttgcgg cttccgaatt
     1921 gaacgtcacg caaaccgcga tcagccatca gatccggcgg cttgaggagg aactggggat
     1981 tcgcctgttc atccggcaaa accgggcgct ggcgctgacg ccggaagcgc gggattatct
     2041 gcccggggtc cgggctgctt ttaacgattt gcggctcgct accgaccggc tgttacgccg
     2101 ggacaacgac cacgtgctga ccgtcagtac gctggcgtcg ctggccgcaa agtggctgct
     2161 gccaaggctc tcggcgttcc aggaagctaa tcccggcatc gacgtccgca tcaccacctc
     2221 gaccgccctt atcgatttca agagcggcga tgtcgatgcc gcgatccgtt atggtcgggg
     2281 gcaatggccg ggcttgcgcg cccactggct gatggccgat gaactgtttc cggtgtgcag
     2341 tccggcgctg ctggcgggaa aaaagccgct gcggcggcca gaagacctgg cggagcatgt
     2401 gctgctgcac aacagcgcag gcaataacga cgattggcgg ctttggctca cggcggcagg
     2461 cctgccggcc aacctctcga agcagcccgg cataaccttc gatctggttt acatgacggt
     2521 gcaggccgcg atcgacggta taggggttgc catgggccgg acctcctacg tcgaggccga
     2581 catcgccaag ggccgcctgg tggtcccgtt caagatcacg ctgccggccg acgccggctt
     2641 ctatctggtt tcacccgagg ccagagcgga ttcgcccaag ctcgccgcat tccggcaatg
     2701 gctgagcgcc tccgtgcaga acaaaatctg aaaaacgatc tccgtgagcc gatatccggt
     2761 cttgaccggc gttttccggt tggtcgtgcc gagcggacat ggcagattgc ggcatcaaga
     2821 agagcaattt gtcggctggc gagaaatctc tccgcatagg ccatgaaaac ggggagaaat
     2881 tcgccatgtc ggaaacgtcc acgtcagaac cgcctcagat caagtcgcgt ctcggcgcga
     2941 tcctgcgtgc gaccagcggc aatttcctcg aacaattcga tttcttcctg ttcggctttt
     3001 atgcctccta tatcgccaag gcgttctttc cctccgagaa cgagaccgcc gcactgctca
     3061 acaccttcgg cgtattctgg ctgggcgccc tgatgcgccc ggtcggcgcg atcgtgcttg
     3121 gcgcctatat cgaccggatc ggccgccgca aggggctgat cgtcacgctg gcgatcatgg
     3181 cgctgggtac ggtgaccatc gcggtttgcc cgacctatgc cacgatcggc gtggccgccc
     3241 cggtgatcgt gctgatcggc cggttgctgc agggcttttc ggccggcgtc gagcttggcg
     3301 gcgtgtcggt ttatctttcc gagatcgcta cgcccggcaa caggggtttc tacacctcgt
     3361 tccagtccgc cagccagcag gtcgcgatct tcgttgcggc aatcatcgga tttgtgctga
     3421 gcgagatgat gccggccgcg actgtcgcgg aatggggttg gcgcattccg ttcttcatcg
     3481 gctgcctgat cattcccttc atcttctttc tgcgccgaac gctggaagaa accccggaat
     3541 tcctggccat gaagaaacat ccaagcacat cggaggtctt cagctcggcg gcggtcaact
     3601 ggaagatcat cctgcttgga atgatgctgg cggccatgac caccgtgacc ttctacttcg
     3661 tcaccgtcta tacgccgacc ttcggcaaga ccgtgctgaa actctccagc caggacgccc
     3721 tgctggtcac cctcctggtt gccgtgacca acttcatctg gaatccggtc gggggcgcac
     3781 tgtccgaccg tatcggccgc aagcccgtgt tgatggccat cgcaggcctc tcgttcgtga
     3841 cggcctatcc cgcgctgctc tggctcacgg ccgccccgac cttcggcaag atgctggcgg
     3901 tggaactgat gttttcgctc tacttcggcg tttacagcgg gacgatgctg ggtgcgctgg
     3961 ttgaaattgt gccgaagcat gtccgcacca cgtgtttctc gctggcgttt gcgctcgcag
     4021 cggcgctgtt cggcaccttc acgccattcg cggcaacaac gctgatcgcc atgaccagcg
     4081 acagggcgtc gcccggctac tggctgatgt gcgccgcggc gagcggcttc atcgccgcag
     4141 tcgtcatcta ccgcagcgga gcgatcgagg cgcgcgaaca ggcggtcgcc gcgcagcagg
     4201 cgtgagacgg ccggccaggc gctcgcctgg ccgccggttt gatttccatg caaacaacga
     4261 taacaagacg gcatggtcga ttttaattgt aagttcgatg tgctggtgat cggcggcggc
     4321 aatgccgcgc tgtgcgctgc gatcagcgcc cggcggaccg gcgcctcggt gctggtgctg
     4381 gaaggcgcgc caaaatttta tcggggcggc aatacccggc atacgcgcaa catgcgctgc
     4441 gcccacgacg ccgcaaccga cattctcacc gggccctata ccgagcagga attctgggaa
     4501 gacctgttgc gcgtgaccgg cggcgagacc gacgaggaac tggcgcgaca catgatcagg
     4561 gagtccaagg acatcctggg ctggatcgtc gaacagggcg tgcgctggca accctcgctg
     4621 ggcggcacgc tgagcctcgg ccgaaccaat tcgttctttc tcggcggcgg ccgggcaatg
     4681 ctcaatgcgc tgtatctgac ggccgagcaa ctcggcgttg aaatcctcta cgacgccgag
     4741 gtgctcgatc tccagatcga caacggcatg tttctctccg ctgacttgaa acagggcgac
     4801 ggccgcgtaa ccgtgcgcgc ctcgaccctg gtcgccgcgg ccggcggatt cgaggccaac
     4861 atcgaatggc tcaaggagta ttggggcgac gccgccgata atttcctgat ccgcggcaca
     4921 ccctataatc gcggctcgat cctcaagatg ctgctgaaca agggcgtcca ggagatcggc
     4981 gatcccaggc aatgccacgc ggtggcgatc gacgcccgcg cgccgaaatt cgacggcggc
     5041 atcatcaccc ggcacgattc cgtggtgttc ggcatcgtcg tcaacaaaca cgccgagcgg
     5101 ttttacgacg agggcgaaga catctggccg aagcgctacg cgatctgggg tcgcctcgtc
     5161 gcggcccagc cggaccagat cgcctacatc atcttcgacg cgagttcgcg caacagtttc
     5221 atgccgacgc tgttcccgcc gatcgaggcc ggctcgatcg ccaagttggc cggcgaattc
     5281 gaactcgatc cggcggcgct ggaaaagacc gtcggcgact tcaatgcggc ggttaggccc
     5341 ggcaccttcg atcacaccat ccttgacgat tgccgcacca agggcctcgc gccgccgaag
     5401 acgcactggg cgcggaaaat cgagacgccg ccttattatg cctacccggt gcggcccggc
     5461 atcaccttca cctatctcgg cacccgcgtg aacaagcagg cgcgcatgct gatgaaggac
     5521 ggcaagcctg ccgccaacat gttcgccgcc ggcgagatca tggccggcaa tgtgctgggc
     5581 aaaggctacg cggccggaat gggcatgacc atcggcagcg tgttcggccg gatcgccgga
     5641 cgggaagcgg cgcaaaatgc aaggaactag acgaggaact agacgaggaa ctagacgagc
     5701 aactcgacaa gcaactcgag gagggatcat gtcacgcatt ccgagagcaa tgcttgccgc
     5761 ggcattgacg gttgcgacgt cggctgtggc aaacgccgcc gagccgatcg ccttgcgcga
     5821 catgggttca ttccatgtcg gcggccggct ggtcgaaata tcaggcaagc cggtcaagga
     5881 aatcaccttc acgcccggcg gcgtgccggc gaaggtcgat cccaacggca cttatcaagt
     5941 cgagcagatg tacgtgcagt attttctgcc cgccaacgag atcggcgcct acccgctgtt
     6001 gctgtggcac ggcggcggtt tgacgggggt gacctacgag accaccccgg acggccgcga
     6061 aggctggctg aattactttt tgcgcaaggg ctggagcgtc tacaattccg acgccgtcga
     6121 gcgcggccgc gccggctggg cgcaatatcc cgatatcttc aagagcgaac cggtattctt
     6181 gaccaccagc aatccgttcg agcggtttcg catcggcgac ggtccgggct cctacgatcc
     6241 agacccggcg aagcggaagc tgatgccggg cagccagttc ccgaaccaag gctacgaaaa
     6301 cttcgtcaag cagaacgtgc cgcgctggac caccaacgat gatgcgacgc tggccgccta
     6361 tatcgccgag atcgatcggg tctgtccgtg catcgtcctg tttcacagcc aggccggcac
     6421 cttcggcttc aaggcggcgc aagcacggcc cgacaaggtc aaggcgctga tcgcggtcga
     6481 gcctgccggc gtcggcgatc ccgcaaggat tgatgcgttg aagggaatcc cctcgctgct
     6541 gatctatggc gactttatcg agcgcgattc gcgctggccc aaaattcgcg ccaccgggat
     6601 cgcctttgcc gacggcatca aggccgccgg cggcagcgcc gatgtcgtcg atctgcccaa
     6661 ggcgggcata tccggcaatt cgcacatgat gatgatggac aagaacaata ccgaggttgc
     6721 cgcgctgatc cagacgtggc tgcaaggcaa agggctcacc aaaaagccct gagccatttt
     6781 tcgaaagcgg aagcggcaca gcatgcacgg aaccaagatc ctggaggaag ccgaccgact
     6841 gatgacggtc tgcaattcgt gccgctattg cgaaggcctt tgcgcggtat ttccggcgat
     6901 ggaaatgcgc cgctcgtttt ccgacggcga cctgaattat ttcgccaatc tctgccacgc
     6961 ctgcggcgcc tgctacaccg actgccagtt ctcgccgccg catgaattca acgtcaatgt
     7021 gccgcagacg ctggccgtcg cccgcaccga ctcctatgcc gcctatgcct ggccgcgcgc
     7081 cttttcgggc gtcttcgcgc gcaacggtct tgcgatcagc atcatcgcgg ccttgagcgt
     7141 cgcggcattt attttcggct tcgcggccct taacgaccgc caggttctgc tgggcatcca
     7201 taccgggccc ggcgcgttct atgcgctgat gccgcataac gcgatggcgg ctttgtttgg
     7261 cgccgcgttc gtgtacgcga ttctggcgct ggtaatgggc gtgcgcgcgt tctggcgcga
     7321 tatcggcgag ccggtcggca tgaagaccga tgcgccgtcg ctgtg
//
LOCUS       DATNOI010000032        11272 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1933222,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000032 DATNOI010000000
VERSION     DATNOI010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11272
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1933222"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1689
                     /locus_tag="VN968_01120"
     CDS             <1..1689
                     /locus_tag="VN968_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HXL27882.1"
                     /translation="GLLILLIAGCGALLLRSPVNAAVDTLAEDTDATPPAWILRARWI
                     FLAAVPSGLLIAVTAHISTDVAAAPLLWVLPLSIYLLTWVLVFQSRPLLPHKWMLMLQ
                     PLAITGVIVLLAVGGEQNLLLTLGGHQLCFFVIAMACHGELARTRPAAKYLTGFYVAL
                     SFGGMVGGLFAGLIAPFTFSWIAEYPILLALAALCRPPGGDERFPRWSAWYWPFLAVL
                     AVILIAPSYSEGKIMAWLDEYRVWVIGAVGVLSALLALGLNANRWKIFATVAVALLLI
                     RAYPSDDGRVETVRSFFGVHKIVVTPHGQYHVLMHGTTIHGAEKFQNDDGTPVTGRPE
                     PITYYHKDGGIGQAITAIRERKGGPLRVAVIGLGSGTLTCASEPGETWKFFEIDQSMV
                     DTARDPKYFTYIRKCEPDLKPVIGDARLTFAREPDSIYDLIIVDAYSSDAIPIHLATE
                     EAMEIYKDKLAPQGAVLMHVSNRHLELSSVVVGIADANDLKSWVYSEDSNRDNEYIFS
                     TSVVVSAREEADIGKLASSEQWALTEADENQRVWTDDYSNVLGAVWRRLRDGEQ"
     gene            complement(1694..2485)
                     /locus_tag="VN968_01125"
     CDS             complement(1694..2485)
                     /locus_tag="VN968_01125"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXL27883.1"
                     /translation="MRSFLVAIAGLMLFTAGAAQAKIDITIDKNAQLMTVAVDGIERY
                     HWPVSTGIPSRETPNGSFRAFRMEEDHFSKEFDDAPMPHSIFFTKIGHAIHGTDSEGR
                     LGTPASHGCVRLSKANAATLYAMVERDGVLNTTVTLTGSSQIALARNPRIRGNTAVAG
                     RDPDQPYAPQYNSAGDPVMLSPLPPPRQIAPVAQPPVQADDDYIYPADGSSDVARYPA
                     PRSSRRLYDAQANPPQQYYDNRGYAPQVYAPRPYYQPRRLFTYQD"
     gene            2806..3126
                     /locus_tag="VN968_01130"
     CDS             2806..3126
                     /locus_tag="VN968_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27884.1"
                     /translation="MMKMKVKTAALPAGLPVKLFLAAGAIVLALLAIPHIGQAQGIVR
                     GAQEGSYEGNRVAGPIGGVVGGAVGAGVGGAMGAVKGVFGITDRGYRCRGYYDRYDRF
                     HCYR"
     gene            complement(3244..4005)
                     /locus_tag="VN968_01135"
     CDS             complement(3244..4005)
                     /locus_tag="VN968_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL27885.1"
                     /translation="MNFRAVRAIYLFEMARTWRTLLQSIVSPVVSTSLYFVVFGAAIG
                     SRITSVEGVSYGTFIVPGLVMLSVLTQSIANASFGIYFPKFVGTIYELLSAPISYFEI
                     VLGYVGAAATKSIILGLIILATAGLFVPLQIHHPWWMLTFLVLTAVTFSLFGFIIGIW
                     ADGFEKLQMIPMLVVTPLTFLGGSFYSVNMLPSGWRTITLLNPVVYLISGFRWSFYEI
                     ADVSVGLSVGITLMFLAICMVTIWWIFKTGYRLKN"
     gene            complement(4002..4925)
                     /locus_tag="VN968_01140"
     CDS             complement(4002..4925)
                     /locus_tag="VN968_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL27886.1"
                     /translation="MPSIISVSNLSKTYGSGFKALNNINLEINRGEIFALLGPNGAGK
                     TTLISIICGIANLSEGSVIVGGHDINKEYRAARSLIGLVPQELHTDAFETVWATVSFS
                     RGLFGKPKNPAHIEKVLTDLSLRDKKDSKIVTLSGGMKRRVMIAKALSHEPQILFLDE
                     PTAGVDVELRKGMWEVVRTLQASGVTIILTTHYIEEAEEMADRIGVINKGEIILVEDK
                     AGLMQKLGKKQLKLHLQGKIDAIPADVAAYDLELCDGGHELIYTYDTNGERTGITSLL
                     SDLRKAGIRFYDLDTTQSSLEDIFVNLVRAS"
     gene            complement(5047..7725)
                     /locus_tag="VN968_01145"
     CDS             complement(5047..7725)
                     /locus_tag="VN968_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXL27887.1"
                     /translation="MTEPNRLTDPGVPEHQPAAGGIAARARAATTPQYLAGLNPEQRE
                     AVETLDGPVLVLAGAGTGKTRVLTCRIAHILSTGRARPAEILSVTFTNKAAREMKLRL
                     GQMLGQAVEGMPWLGTFHSIGGRILRTHAELVQLKSNFTVLDVDDQVRLLKQLLQADN
                     IDDKRWPARMLAGLIDGWKNRGLTPSQVPPGEAAVFGNGRGGKLYATYQERLKVLNAA
                     DFGDLLLENIRLFREHPDVLRQYQNRFKFILVDEYQDTNVAQYLWLRLLSQAPARPGA
                     PLSSIIPGHATFPSPLVGEGAEPQSGEAGEGPREAPLHEEAPSPGVSFHSPPPSPTRG
                     EGKEVTPSSPPKNICCVGDDDQSIYGWRGAEVDNILRFDHDFPGAKVVRLERNYRSTG
                     HILAAASHLIAHNESRLGKTLRTEDVDGEKVTVTGSWDSEEEARAIGEEIEELQRGGH
                     KLNDIAIMVRASFQMREFEDRFVTLGLPYRVIGGPRFYERAEIRDALAYLRVINSPAD
                     DLAFERIVNVPKRGLGDATVQMLHDHARKRRIPLSEAARAVVETDELKPKARGSLRDL
                     LVNFDRWRAQREVTSHTELAEIVLDESGYTEMWQKDRSADAAGRLENLKELVRSMEEF
                     ENLQGFLEHISLVMDRDGGAEDDAVSLMTLHSAKGLEFDNVFLPGWEEGLFPSQRTLD
                     EQGRAGLEEERRLAHVGLTRARRRAKLYFATNRRIHGTWSTTIPSRFLDELPAHNVEI
                     TESKGGSGWGGASGYGPSRFDNVESFGSSYSTPGWQRAQANRARGQTGRNGQSGQAGG
                     GFNESQSPFSGSRSDASARSDAGGFARNKRSPMTIEGELVAKSTGTTSEFSLDDRVFH
                     QKFGYGNVVKVDGNKLTIAFEKAGEKKVVDSFVERV"
     gene            8027..8200
                     /locus_tag="VN968_01150"
     CDS             8027..8200
                     /locus_tag="VN968_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HXL27888.1"
                     /translation="MLSWVVTFLVIALIAGILGFGGIAGASVEIAKIIFFIAVVLFLV
                     SAVVGVARGRTRV"
     gene            complement(8205..8717)
                     /locus_tag="VN968_01155"
     CDS             complement(8205..8717)
                     /locus_tag="VN968_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXL27889.1"
                     /translation="MTALTAGDNVLLRPEPFRASVMQIEPQWIDYNGHLNMAYYNVMF
                     DRAIDELWLKIGIGPGYMKERNGSTFTAECHVRYLREIHLGDPAQVSILLVAADEKRL
                     HTFKELRHATEGWLSATSENMTIHIDMAARKTAPFPPDIRARIQALANAHATIPRPEG
                     IGRRVAMPSK"
     gene            8800..10218
                     /locus_tag="VN968_01160"
     CDS             8800..10218
                     /locus_tag="VN968_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXL27890.1"
                     /translation="MGLTITNNLKRPEPQALAATVEALAARFGNRLITSRAVREQHAH
                     TTTWLASQPPDAVVMAQETSDIQDTVRICAKYGVPVIAFGTGTSLEGQVNAPAGGVCI
                     DLRDMNRVLEVHAEDLDCVIQPGVTRKGLNEHLRDQGLFFPIDPGADASLGGMASTRA
                     SGTNAVRYGTMRDNVLALKVVRGDGEIITTGTRAKKSSAGYDLTHLFVGAEGTLGIIS
                     ELTIKLRGIPETIAAAACSFESVRGACQATILAIQTGIPVARIELLNAAQVQACNSYS
                     KLSLPETPLLLLEFHGSANEVAEQSRNFSEIAKECGGGDFTWTTRPEDRTKLWQARHD
                     AYWAVKALRPGAGVVATDVCVPISRLADCVTETEADLKRLNLQSPIVGHVGDGNFHCS
                     LLCDVDDRDEMARGEDFMHRLVERAQSMGGTCTGEHGIGQGKQKYLKAELGPEAIDAM
                     RALKLALDPQNIFNPGKILPTA"
     gene            10474..>11272
                     /locus_tag="VN968_01165"
     CDS             10474..>11272
                     /locus_tag="VN968_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL27891.1"
                     /translation="MKRREFLKTAGAGLAASTAVAAPAIAQSMPELKWRCTTSWPKSL
                     DVPFSASETISKYVAEATDNKFQIQAFAGGEIVPALQALDAVSNATVEMCHTAAYYFI
                     GKDPTFALYCSVPFGLNSRQQNAWFQDHGGQDMLNEFGKKYKLYGISGGNTGTQMGGW
                     FRKEINTVGDLNGLKMRIGGWAGKTLSKLGTIPQQIAGGDIYPALEKGTIDATEWVGP
                     YDDEKLGFYKVAKYYYYPGWWEGGTALHFFINSEKWDALPKAYKSLLT"
BASE COUNT         2023 a         3371 c         3679 g         2199 t
ORIGIN      
        1 ggattgctga ttttgctgat cgccggctgc ggcgcgctgt tgctgcgctc gccggtgaat
       61 gcggcggtgg atacgcttgc ggaggatacc gacgcgacgc cgcccgcctg gatcttgcgc
      121 gcgcgctgga tttttcttgc cgcggtgccg tcgggcctct tgatcgcggt caccgcgcat
      181 atctccaccg acgtcgcggc ggcgccgctg ttgtgggtgc tgccgctgtc gatctatctc
      241 ctgacctggg tgctggtgtt ccagtcgcgt ccgctgctgc cgcataaatg gatgctgatg
      301 ctgcagccgc tggcgatcac cggcgtcatc gtgctgctcg cggtcggcgg cgagcagaat
      361 ctgctgctga cgcttggcgg gcaccagctt tgtttctttg tcatagcgat ggcctgccat
      421 ggcgagttgg cgcggacccg cccggccgcg aaatatctca ccggctttta tgtcgcgttg
      481 tcgttcggcg gcatggtcgg cggattgttt gcaggcctga tcgcgccgtt cacgttttca
      541 tggatcgcgg aatatccgat cctgctggcg ctggcggcat tgtgccgccc gcccggcggc
      601 gacgagcgtt ttccccgctg gagcgcctgg tattggccgt ttctcgccgt gctcgcggtg
      661 atcctgatcg cgccctctta ttcggagggc aagatcatgg cctggctcga cgagtaccgg
      721 gtctgggtga tcggcgccgt cggcgtgctc tcggcactgc tggcgctcgg attgaacgcc
      781 aaccgctgga agattttcgc gaccgtcgcg gtggcgctgc tgctgatccg cgcctatccg
      841 tcggatgacg ggcgggtgga aaccgtgcgc agctttttcg gcgtccacaa gatcgtggtg
      901 acgccgcacg gccagtatca cgtgcttatg cacggcacca cgattcatgg cgccgagaaa
      961 tttcagaacg acgacggcac gccggtgacc gggcgaccgg agccgatcac gtattaccac
     1021 aaagacggcg gcatcggcca ggcgatcacg gcgattcgcg agcgcaaagg cggaccgctg
     1081 cgggtcgcgg tgatcggcct cggctccggc accctgacat gcgcgtcgga gccgggcgag
     1141 acctggaaat tcttcgagat cgatcaatcg atggtcgata ccgcgcgtga tcccaaatat
     1201 tttacctaca tccggaaatg cgagccggat ctgaagcccg tgatcggcga cgcgcggctg
     1261 accttcgccc gcgagccgga cagcatctat gatctgatca tcgtcgatgc ctattcgtcg
     1321 gacgcgatcc cgattcacct ggcgaccgaa gaggcgatgg agatctacaa ggacaagctc
     1381 gcgccgcagg gcgcggtgct gatgcatgtc tcgaaccggc atctggagtt gtcgagcgtc
     1441 gtggtcggca tcgccgacgc caacgatctc aagagctggg tctacagcga ggattccaac
     1501 cgcgacaatg aatatatctt ctcgacctcg gtcgtggtct ccgcgcgcga ggaggccgat
     1561 atcggcaagc tcgcatcgtc tgaacaatgg gcgctgaccg aggccgacga gaatcagcgg
     1621 gtctggaccg acgattattc caacgtgctc ggcgcggtat ggcggcgcct gcgcgacggc
     1681 gagcagtgaa aggttagtcc tgatacgtga acagtctgcg cggctggtaa tatggccgcg
     1741 gcgcgtagac ctgcggcgcg tatcctcggt tatcgtagta ttgctgcggc ggattggcct
     1801 gcgcgtcgta gaggcgccgg gagctgcgcg gcgcgggata gcgcgccacg tcagagctgc
     1861 cgtcggccgg atagatgtag tcgtcgtcag cctgcacggg aggctgcgcc accggcgcga
     1921 tctggcgggg cggcggcagc ggagacagca tcaccggatc accggctgaa ttatactgcg
     1981 gagcgtatgg ctgatccgga tcgcggccgg caacggcggt attgccgcgg atccgcgggt
     2041 tgcgcgccag tgcgatctgg gaggagccgg tgagcgtcac ggtggtgttg agcacgccat
     2101 ctctctcgac catcgcatac agcgtggcgg cgttggcctt cgacagacgc acacagccat
     2161 gggacgcggg cgtgccgagg cggccttcgg agtccgtgcc gtggatggcg tggccgatct
     2221 tggtgaagaa gatcgagtgc ggcatcggcg cgtcatcgaa ttccttggag aagtggtctt
     2281 cctccatgcg gaaggcgcgg aagctgccgt ttggcgtctc gcgcgatgga atgccggtcg
     2341 agaccggcca gtgatagcgc tcgatgccgt cgaccgcgac ggtcatcagc tgcgcattct
     2401 tgtcgatggt gatgtcgatt ttggcctgcg cggcgccggc ggtgaacaac atcaggccgg
     2461 cgatagcaac gaggaaggaa cgcattttac tcttggcctc caggttactc ttggcctcca
     2521 ggccttcact gcgacgtctt gactgcggcg ccgccaccgt accccgcatc aatatgcccc
     2581 gaattcggct ttggttccag cggttcgtcg gtcaatcgtt cactgtctgg cagggcgcaa
     2641 acaaggcggc ggccggtctg aatgcggcgg gccgcgcaat ttttcgggag gcggcgcctt
     2701 gtcgcgctat cctgaattta ccacagaggc gcaacctttt gcccgtatcg gcgttacaag
     2761 ttcccgccgc gccggtcggt tgcggtcgag ccccaaggaa ttcagatgat gaagatgaag
     2821 gtcaagacag cggcgctgcc ggcagggttg cccgtaaagc tctttcttgc ggccggggcc
     2881 attgtgctgg cattgcttgc gattccccat atcgggcagg cccagggcat cgtgcgcggc
     2941 gcgcaggaag gttcctacga gggcaaccgg gtcgccggac cgatcggtgg tgtggtcggc
     3001 ggggcggtcg gagccggtgt cggcggcgcg atgggggccg tgaagggcgt gttcggcatc
     3061 acggaccgcg gctaccgctg ccgcggctat tacgaccgtt acgatcgctt ccactgctat
     3121 cgatgaattt ttaagacgtc attccggggc acgctttagc gcgaacccgg aatctcgaga
     3181 ttccgggctc gcgtcttcga cgcgccccgg aatgacgcgt tgagaattgc ggtgcgcttc
     3241 gcgtcagttc ttcagccgat aaccggtctt gaaaatccac catatcgtga ccatgcagat
     3301 ggcgagaaac atcagcgtga tgccgacact caggccgacg ctgacgtcgg cgatttcgta
     3361 aaagctccag cggaagccgc tgatcagata gaccacggga ttgagcagcg tgatggtgcg
     3421 ccagcccgag gggagcatgt tcaccgaata aaagctgccg ccgaggaacg tcagcggcgt
     3481 gaccaccagc atcgggatca tctgcagctt ctcgaagccg tcggcccaga tcccgatgat
     3541 gaagccgaac aggctgaagg tcaccgcggt cagcaccagg aacgtcagca tccaccaggg
     3601 atgatgaatc tgcagcggca cgaacaggcc ggcggtggca agaatgatca ggccgaggat
     3661 gatcgatttg gtcgcggccg cgccgacata gccgagcacg atctcgaaat acgagatcgg
     3721 cgccgacagc aattcgtaga tcgtgccgac gaatttcgga aagtagatgc cgaacgaggc
     3781 attggcgatg ctctgcgtca gcaccgacag catcaccagc ccgggcacga tgaaggtgcc
     3841 gtagctgacg ccttcgaccg aagtgatgcg cgagccgatc gccgcaccga acaccacgaa
     3901 atacagcgac gtcgaaacca ccggcgaaac gatgctctgc agcagcgtgc gccaggtgcg
     3961 cgccatttcg aacagataga tcgcgcggac cgccctgaaa ttcatgacgc cctcaccagg
     4021 ttgacgaaga tgtcttccag cgaactctgg gtggtgtcga ggtcgtagaa gcggatgccg
     4081 gctttgcgga gatcgctgag caggctggtg atgccggtgc gttcgccgtt ggtgtcgtag
     4141 gtgtaaatca gctcgtgtcc cccgtcgcat agttcgagat cataggccgc gacatcggcg
     4201 gggatcgcat cgatcttgcc ctgcagatgc agcttcagtt gcttcttgcc gagcttttgc
     4261 atgaggcccg ctttgtcctc gaccaggatg atctcgccct tgttgatgac accgatgcgg
     4321 tcggccatct cctcggcctc ttcgatgtaa tgcgtggtga ggatgatggt gacgccggag
     4381 gcctgcagcg tccgcaccac ttcccacatg cccttgcgca attcgacgtc gacgcccgcg
     4441 gtcggctcgt cgagaaacag gatctgcggc tcgtgcgaca gcgcttttgc gatcatcacc
     4501 cggcgcttca tgccgccgga cagcgtgacg atcttggaat ccttcttgtc ccgcagcgac
     4561 aggtccgtca gcaccttttc gatatgtgcc gggtttttcg gcttgccgaa caggccgcgg
     4621 ctgaaactga cggtcgccca caccgtctcg aaggcgtcgg tgtgcagttc ctgcggcacc
     4681 agcccgatca gcgagcgcgc cgctcgatat tccttgttga tgtcatggcc gccgacgatg
     4741 acgcttcctt cgctgagatt ggcgatgccg cagatgatgc tgatcagtgt ggttttgccg
     4801 gcgccgttcg gcccgagcag cgcaaaaatc tcgccgcggt tgatctccag attgatattg
     4861 ttcagcgcct tgaaaccgga gccataagtc ttggacagat tggagacgga tataatggaa
     4921 ggcatgacgg ctttgatggt ctgagttgga aatgcgggaa cggatgaagc ggtgcttcag
     4981 ccagctcagc ggcagcaggt cggcgaggtc caacagatag gaacgcttga gccattgcgc
     5041 aatcggtcac acccgctcga caaaactatc gaccaccttt ttctcgccgg ctttttcgaa
     5101 ggcgatggtg agcttgttgc cgtcgacctt gacgacgttg ccgtaaccga atttctggtg
     5161 gaatacgcga tcgtccagtg aaaactccga tgtggtgccg gtggatttcg cgaccagttc
     5221 gccttcgatg gtcatcggac tgcgcttgtt gcgtgcgaag ccgccagcat cgctgcgcgc
     5281 ggaggcatcg cttcgcgagc ctgagaatgg cgactggctc tcattaaagc cgccgccggc
     5341 ctggccgctc tgtccattgc ggccagtttg gccacgcgcg cggttggctt gcgcgcgctg
     5401 ccagcccggc gtcgaatagc tcgagccgaa cgattccaca ttgtcgaagc gcgaggggcc
     5461 gtagccgctg gcaccgcccc agcccgaacc gcccttggat tcggtgatct cgacattgtg
     5521 tgccggcaat tcgtcgagaa agcgcgacgg gatcgtggtc gaccaggtgc catgaatccg
     5581 acggttggtg gcaaaataaa gtttggcgcg tcggcgcgcg cgggtcagcc cgacatgggc
     5641 gaggcggcgc tcttcttcga ggccggcgcg gccctgttcg tcgagcgtgc gctggctcgg
     5701 aaaaaggcct tcttcccagc ccggcaggaa cacgttgtcg aattccagcc ccttggccga
     5761 atgcagcgtc atcagcgata ccgcgtcgtc ctcggcgccg ccgtcgcggt ccatcaccag
     5821 cgagatgtgt tcgagaaagc cctgcaggtt ctcgaactcc tccatcgagc gcaccagctc
     5881 cttgaggttt tcgaggcgtc cggcggcgtc ggcggagcga tccttctgcc acatttcggt
     5941 gtagccgctt tcgtcgagca cgatctcggc cagttcggtg tgcgaggtca cctcgcgctg
     6001 cgcacgccag cgatcgaaat tgacgaggag gtcgcgcagg gaaccgcgtg ccttcggctt
     6061 cagctcgtcg gtttcgacca cggcgcgggc ggcctcggag agcggaatcc ggcgcttgcg
     6121 ggcgtggtcg tgcagcatct gcacggtggc gtcgccgagg ccgcgcttcg gtacgttgac
     6181 aatgcgctcg aaggcgagat cgtcggcggg cgagttgatc acgcgcaaat aggccagcgc
     6241 gtcgcggatt tcggcgcgct cgtagaacct cgggccgccg atcacgcgat agggtagccc
     6301 gagcgtgaca aaacgatctt cgaattcgcg catctggaac gacgcccgca ccatgatcgc
     6361 aatgtcgttg agcttgtggc cgccgcgctg cagctcctcg atctcctcgc cgatggcgcg
     6421 ggcttcctct tcggaatccc aggagccggt gacggttact ttttcgccgt cgacgtcctc
     6481 ggtgcgcagc gtcttgccga gccggctctc attatgcgcg atcagatgcg aggcggcggc
     6541 caggatgtga ccggtggagc ggtagttgcg ctcgaggcga acgaccttgg cgccgggaaa
     6601 atcatggtcg aagcgcagga tattgtcgac ctcggcaccg cgccagccat agatcgactg
     6661 gtcgtcatcg ccgacgcagc agatgttttt gggcggcgag gagggcgtca cctctttccc
     6721 ctcgcccctt gtgggagagg gtggcggcga gtgaaacgag acgccgggtg agggggcctc
     6781 ttcatgaaga ggcgcttcgc gcggcccctc accggcctca ccgctttgcg gttcggcacc
     6841 ctctcccaca aggggagagg ggaaggtggc gtgccccggg atgatggaag aaagcggcgc
     6901 gcccggcctc gccggtgcct gcgacaacag ccgcagccac agatactgcg ccacgttggt
     6961 gtcctgatat tcatccacca gaatgaactt gaagcggttc tggtactgcc ggagcacgtc
     7021 gggatgttcg cgaaacagcc ggatgttttc cagcagcaga tcgccgaagt cggcggcgtt
     7081 gaggactttt agccgttcct gataggtcgc gtagagcttg ccgccgcggc cgttgccgaa
     7141 taccgcggct tcgcccggcg gcacctgcga cggcgtcagc ccgcgattct tccagccgtc
     7201 gattaatccg gccagcatgc gcgccggcca gcgcttgtcg tcgatattgt ccgcctgcaa
     7261 gagctgtttc agcaggcgaa cctgatcgtc gacatcaagc acggtgaaat tggatttcag
     7321 ttgcaccagt tcggcgtggg tgcgcaggat gcgtccgccg atcgaatgaa aggtgccgag
     7381 ccacggcatg ccttcgacgg cctggccgag catctggcca aggcgcagtt tcatctcgcg
     7441 ggccgccttg ttggtgaagg tgaccgacag gatttcggcg gggcgggcgc ggcccgtgct
     7501 gagaatgtgg gcgatgcgac aggtcagcac ccgggttttg ccggtgcccg cgcccgccag
     7561 caccaagacc ggaccatcca gcgtttccac cgcctcgcgc tgctcgggat tgagcccggc
     7621 aagatattgc ggggtcgtgg ccgcgcgggc gcgcgcggca atgccaccag ccgcaggctg
     7681 atgctcggga acgccgggat cggtcagtct gttcggctca gtcatgcgaa tcgtcattcg
     7741 cccacgatgg caccgcgggg ccttgaaggg gagccttcat agcaggattg aagggcatat
     7801 aggggcttgg cggcggattt gacaggttta tcccgtcgtc cccgcagggg tgaatttcgc
     7861 catccagcct ccaatttgtt ccccgcgaat cgacattttc agtccgcaga gcacccggac
     7921 ctacgcggaa catccgttgc gaatgtggat tatttccgtg gccgatgcgc gatgctcttc
     7981 gtcgcgccga ttttgaaaca caaggtgcaa aacagaggtt ggtgtcatgc tgagctgggt
     8041 ggttacgttt ttggtcatcg cgctgatcgc gggcattctg ggttttggcg ggatcgccgg
     8101 agcctccgtc gaaatcgcca agatcatctt cttcatcgcg gtcgtgctgt tcctggtctc
     8161 ggccgtggtc ggcgtggcgc gcggacgtac ccgcgtctga cgcgctattt cgagggcatc
     8221 gcgactcttc ggccgatgcc ctcgggtcgt ggaatggtcg cgtgggcatt cgccaaagcc
     8281 tggatgcggg cgcgaatatc gggcggaaac ggtgcggtct tgcgcgcggc catgtcgata
     8341 tgaatggtca tgttttccga ggtcgcggac agccagccct cggtggcgtg gcgcaattcc
     8401 ttgaaggtat gcagccgctt ttcgtcggcg gcgaccagca ggatcgaaac ctgcgcggga
     8461 tcgccgagat gaatctcgcg caagtaccgc acgtggcatt cggcggtgaa agtcgagccg
     8521 ttgcgctcct tcatgtatcc ggggccgatc ccgatcttga gccataattc gtcgatggcg
     8581 cggtcgaaca tcacattgta ataggccatg ttgagatggc cattgtagtc gatccattgc
     8641 ggctcgatct gcatcaccga ggcccgaaac ggttcgggcc ggagcagcac attgtcgccg
     8701 gcggtgagcg ccgtcatcat tgatccccgt cggttcggtt gatgatccct tgacccatta
     8761 tatatccttt gtcacggtct tccgccggga ggatatcgcg tgggtctcac catcaccaac
     8821 aatttgaagc ggccggaacc gcaggcgctg gcggcgacgg tcgaagcgct cgccgcgcgg
     8881 ttcggcaaca ggctgatcac ctcgcgggcg gtgcgcgagc agcacgcgca taccaccaca
     8941 tggctggcat cgcagccgcc cgacgcggtc gtcatggcgc aggagacatc ggatattcag
     9001 gacacggtgc ggatctgcgc caaatacggc gttcccgtga tcgcgttcgg caccggcacc
     9061 tcgctggagg ggcaggtaaa cgcgccggct ggcggcgtct gtatcgatct gcgcgacatg
     9121 aaccgggtgc tcgaggttca cgccgaggat ctcgactgcg tgatccagcc cggcgtcacc
     9181 cgcaaggggc tgaacgagca tctgcgcgac cagggattgt tctttccgat cgatcccggc
     9241 gccgatgcgt cgctgggcgg catggcgtcg acccgggcgt ccggcaccaa tgcggtgcgc
     9301 tacggcacca tgcgcgacaa cgtgctggcg ctcaaggtgg tgcgcggcga tggcgagatc
     9361 atcaccaccg ggacgcgcgc gaagaaatcc tcggccggtt acgacctgac gcatctgttt
     9421 gtcggcgccg aaggcacgct cgggatcatt tcagagctca ccatcaagct gcgcggtatt
     9481 cccgaaacca ttgcggccgc ggcctgttcg ttcgaaagcg tgcgcggcgc ctgccaggcg
     9541 acgattctgg cgatccagac cggcattccg gtcgcgcgga tcgagctgct caacgcggcg
     9601 caggtgcagg cctgcaattc ctattcgaaa ctgtcgttgc cggagacgcc gctgctgctg
     9661 ctggaatttc acggcagcgc caatgaagtc gccgaacaat cgaggaattt cagcgagatt
     9721 gcgaaggagt gcggcggtgg cgatttcacc tggaccacca ggccggagga tcgcaccaag
     9781 ctctggcagg cgcggcacga cgcctattgg gcggtgaagg ccctgcgtcc cggcgcaggc
     9841 gtggtggcga ccgatgtctg cgtgccgatt tcgcggctgg ccgattgcgt gaccgaaacc
     9901 gaggccgacc tcaagcgcct caatctgcag tctccgattg tcggccatgt cggtgacggc
     9961 aatttccact gctcgctgtt atgcgacgtc gacgatcgcg acgagatggc gcgcggcgag
    10021 gacttcatgc atcgcctggt cgaacgcgcg caatcgatgg gcggcacctg caccggcgag
    10081 cacggcatcg ggcagggcaa gcagaaatac ctcaaggccg aactcggccc cgaggcgatc
    10141 gacgccatgc gcgcgctgaa gctggcgctc gatccgcaga acatcttcaa tcccggtaag
    10201 atattgccga cggcgtaggg tcgttattcg gcgccaagtt tcgagcagcg atcaataaat
    10261 gaaccgcgtt ggccgcgcgc atctaaccgg cgaagatcaa ttccggtgag tgaagctacc
    10321 ccgcagcctc aacctctggt accgtcggta gcgttccaga ggagcggccg gggaaagtcg
    10381 gttgagatga aaacctgatc gctggtgggc ggctgcgcga aatcgccgcg ggattgctgt
    10441 ttcaaaaaca taaagcaaac gggggagaac gccatgaaac gtcgtgaatt tctgaaaacg
    10501 gcaggcgccg ggctcgccgc gtcaaccgct gtcgccgcac ccgccatcgc gcagtcgatg
    10561 cccgaactga aatggcgctg caccacgagc tggccgaagt cgctcgacgt tcccttcagt
    10621 gcgtcggaga cgatttcgaa atatgtcgcg gaagccaccg acaataaatt ccagattcag
    10681 gctttcgccg gcggcgagat cgttccggcg ttgcaggcgc tcgatgcggt gagcaacgcc
    10741 acggtcgaga tgtgccacac cgccgcctat tacttcatcg gcaaggaccc gaccttcgca
    10801 ctctattgct ccgttccgtt cgggctgaat tcgcgtcagc agaatgcctg gttccaggat
    10861 cacggcggcc aggacatgct caatgaattc ggcaagaagt acaaattgta cggcatctcc
    10921 ggcggcaaca ccggcaccca gatgggcggc tggtttcgca aggagatcaa caccgtcggg
    10981 gacctcaatg ggctcaagat gcggatcggc ggatgggcag gcaaaacact ttccaagctt
    11041 ggcaccattc cgcagcagat cgcgggcggc gatatctatc cggcattgga aaagggcacg
    11101 atcgacgcca ccgaatgggt cggcccctac gacgatgaga agctgggctt ctacaaggtc
    11161 gccaaatact actactatcc cggctggtgg gagggcggca cggcgctgca tttcttcatc
    11221 aactcggaga aatgggacgc gcttccgaaa gcttacaagt cgctgctcac ga
//
LOCUS       DATNOI010000033         2635 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2659325,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000033 DATNOI010000000
VERSION     DATNOI010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2659325"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VN968_01170"
     CDS             <1..208
                     /locus_tag="VN968_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27892.1"
                     /translation="IVNAGRGTEEHAAALRKIANVLCKRDGVEAIVLAGTELALVFNE
                     NNTDFPAIDCARLHIDGIMREITG"
     gene            398..1402
                     /locus_tag="VN968_01175"
     CDS             398..1402
                     /locus_tag="VN968_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL27893.1"
                     /translation="MAFVDPALPRILSVISLAVLALCALAASPQQARAETSYPDKAVR
                     IVLPFAAGGVADITARIVAEKLGDKLGQRFYVENQAGAGGIAAARTVISSAPDGYTLA
                     LLSNGTAVSVSLFKKLPFDPLRDFEPISSLGFFDFVFATGAGSEFRTLAEFIAAAKAR
                     PGALNVGTINIGSTQNLSAELFKTAAAIDFTIIPYRATPEIQVALLQGDIALMIDSYS
                     SMKGNLADNKFRALASSGPVRSESTPDVATVRESGVADYDVVSWNALFAPAGTPAEIV
                     NKLDGALRDILADAEVKKRLIELGIEAKASTPQEISARLTSDIDKWQKVIEKAGIQKQ
                     "
     gene            1461..2393
                     /locus_tag="VN968_01180"
     CDS             1461..2393
                     /locus_tag="VN968_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HXL27894.1"
                     /translation="MPTNPKRWHVGLVGYGEVGRILAEDLRQQDVGITAYDLKLGNDQ
                     AGPLRDHASAQGVALAASHADLAAKADFIVSAVTASQAVAVAQACAPAVKKGAWFLDF
                     NSASPGAKRRAAELIDGSGGRYVEGAVMTSIPPYRIKVPLLLGGAGAEELAPLLVALG
                     FDAKVASKELGVASAVKMCRSVMIKGLEAMVIESFTTARAYGVEDAVVASLKETYPGI
                     DWEKQGAYFFQRVIEHGRRRSEEVREVAETVREIGLTPWSAQGTAERQAWVADLADEG
                     LFGPKGTAAFARSPDWRTEADRILAAIEQPKNCT"
     gene            2414..>2635
                     /locus_tag="VN968_01185"
     CDS             2414..>2635
                     /locus_tag="VN968_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL27895.1"
                     /translation="MEFQKTPGWLDWYADPSKPRFKVPAGAVDAHCHVFGPGAEFPFA
                     AERKYTPCDASRRQLFALRDHLGFARNVIV"
BASE COUNT          496 a          796 c          851 g          492 t
ORIGIN      
        1 gattgtgaat gctggacgag ggaccgaaga gcacgcggcg gctcttcgca agattgccaa
       61 tgtgctctgc aaacgtgacg gtgttgaagc gatcgtgttg gctggcaccg agctggccct
      121 ggtattcaac gaaaataaca ctgactttcc ggcaatcgac tgcgcacggt tgcacattga
      181 cggaatcatg cgggagataa ctggttgatg cagatcgaga gcttccgtgc ggcgacgctt
      241 tgggtcttta gcgtcgattt ccgattgtcc gccatatgtc gcgcgctggt tgtgggaaaa
      301 tcgggaaacg ttagagcggc ggggagtttg gagcggcgcg gtcgcttgct ataaatgttg
      361 gtcgctctaa cttcgttcac ggaagcaaaa attatccatg gcatttgttg atcccgccct
      421 cccccgcatt ctctccgtga tatctctggc cgtgcttgca ctatgcgcgc tggcagcctc
      481 gccacagcaa gcccgcgcgg aaaccagcta tccggacaag gcggtgcgga tcgttctgcc
      541 gtttgcggcc ggcggtgtgg ccgatatcac ggcgcgcatt gtcgcggaaa aactcggcga
      601 caagctggga cagcgctttt acgtcgagaa tcaagcgggg gccggcggca tagcagcggc
      661 gcggacggtg atctcatctg cgcccgacgg ctataccctg gcgctgctgt ccaacgggac
      721 cgcggtcagc gtctccctgt tcaagaagct gccgttcgat ccgctccgtg atttcgagcc
      781 gatctccagt ctgggatttt tcgatttcgt gtttgcgacc ggcgccggct cggaattcag
      841 gacgctggca gaatttatcg ccgcggcaaa agccaggccc ggcgcgctca atgtcgggac
      901 catcaatatc ggcagcaccc agaacctgtc ggccgagctg ttcaagaccg cggccgccat
      961 cgatttcacc atcatcccct atcgggcaac acccgagatc caggtcgcgc tgctgcaagg
     1021 cgatattgcg ctcatgatcg acagctactc gtcgatgaag ggcaaccttg ccgacaacaa
     1081 gttccgggcg ctggcatcgt cgggtccggt gcggtcggaa agtacgcctg atgttgcgac
     1141 ggtgcgcgaa agcggcgttg ccgactacga cgtggtctcc tggaacgcgc tgtttgcgcc
     1201 tgccggcacg ccggcggaga tcgtgaacaa gctcgatggc gcgttgcggg acatactcgc
     1261 cgacgcggag gtcaaaaagc ggctgatcga acttggcatc gaggccaagg caagtacgcc
     1321 gcaggaaatt tcagcccggc tcacatccga catcgacaag tggcagaagg tgatcgagaa
     1381 ggccggaatt caaaagcagt agccgcgttg ccttgacgag gcgtccgatc cggatctgga
     1441 gattgtttgc aatgaccgag atgccgacca acccgaagcg ctggcatgtg ggccttgtcg
     1501 gctatggcga agtgggccgc attcttgccg aagacctgcg ccagcaggac gttgggatca
     1561 ccgcctacga cctcaagctc ggcaacgacc aggccgggcc gttgcgcgat cacgcctcgg
     1621 cgcagggcgt cgcgctggcg gcttcccatg ccgatctcgc agcaaaagcc gacttcatcg
     1681 tttccgccgt cacggcgagc caggcggtcg cggtcgccca ggcttgcgcg cctgcggtga
     1741 agaaaggcgc ctggttcctc gacttcaatt cggcgtcgcc gggcgccaag cgtcgggcgg
     1801 ccgagctgat cgacggcagc ggcggtcgct atgtcgaagg cgccgtgatg acgtcgatcc
     1861 cgccctaccg catcaaggtg cccttgctgc ttggcggcgc cggcgccgag gaactggcgc
     1921 cgttgctggt ggcattaggc ttcgacgcca aggttgcgag caaggaactg ggcgtcgcct
     1981 cggccgtcaa gatgtgccgc agcgtcatga tcaagggcct cgaggcgatg gtgatcgaga
     2041 gtttcaccac cgcgcgggcc tacggcgtcg aggacgcggt agtggcctcg ctcaaggaaa
     2101 cctatcccgg catcgactgg gagaaacaag gcgcctactt cttccagcgg gtgatcgaac
     2161 atggccggcg ccgcagcgag gaagttcgtg aggtggccga aaccgtgcgc gagatcggcc
     2221 tgacgccgtg gtcggcgcaa ggcacggccg aacgccaggc ctgggtggcc gacctcgccg
     2281 atgagggcct gttcggtccg aaaggcacgg cggcgtttgc ccgcagcccg gactggcgca
     2341 ccgaggccga ccgcatcctc gccgcgatcg agcagcccaa gaactgcact taagaactga
     2401 gcgattaaag cccatggaat tccagaagac tcccggctgg ctggactggt atgccgaccc
     2461 ctcgaagccc cggttcaagg tgccggctgg tgccgtggac gcgcactgcc atgttttcgg
     2521 ccctggagcc gaattcccct ttgcggccga gcgcaaatac acgccctgcg atgcttcaag
     2581 gcggcaatta tttgccctgc gcgatcatct cggcttcgcg cgcaacgtca tcgtg
//
LOCUS       DATNOI010000034        10717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2493518,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000034 DATNOI010000000
VERSION     DATNOI010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10717
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493518"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VN968_01190"
     CDS             <1..802
                     /locus_tag="VN968_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL27896.1"
                     /translation="ADDGYDDFQAQMAEYFKSPNKHPPTVPETAAYYRAKKIVAVIFP
                     VDAERETGFRRYSNDEMLEVAADNSDVLIPFVSIDPHKGKLGVREARRLIEEYGVKGF
                     KFHPTMQGFYPNDRMAYPLYEAINDGGAIALFHTGQTGVGSGMPGGMGMRLKYSNPMY
                     MDDVAADFPDLKIILAHPSFPWQEEALSVATHKPNVHIDLSGWSPKYFPPILVRYINS
                     ILQDKMLFGSDWPVITPDRWLSDFAKLEIRDEIRPKVLKANARRLLNI"
     gene            823..1569
                     /locus_tag="VN968_01195"
     CDS             823..1569
                     /locus_tag="VN968_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HXL27897.1"
                     /translation="MSIKAVVFDAYGTLYDIQSVAAVTEQAFPGYGEIITQVWRIKQL
                     EYTWLRSLMRRYQDFSVITRDSLSYTLRCLGLKHDPAVFERIMEKYLHLDLYPDAIAA
                     LAALRGRKLAILSNGSTGMLDAVVRNSGLDRVLDATISIDSQRIFKPSPDAYALIESR
                     LGVPPAEVLFVSSNPWDACGAKAFGLNVAWIERVTPEAMALACVKSDVVAPLTMFKAI
                     RTQMDELELEPDYRIHALSELPDLVAAHEP"
     gene            1559..2026
                     /locus_tag="VN968_01200"
     CDS             1559..2026
                     /locus_tag="VN968_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HXL27898.1"
                     /translation="MSLESVRAFFADKAPDIAVIESPISSATVALAAEAYGVEPGRIA
                     KTLSLRIGERVVLIIASGTSRMDNKKLKARFGGKPKMLGVDEVADITGHEVGGVCPFG
                     LKTPLPVYCDVSLKVFDEVVPAAGSTHSAVRIAPSRMAELVAAEWVDVCQEPA"
     gene            complement(2129..2365)
                     /locus_tag="VN968_01205"
                     /pseudo
     CDS             complement(2129..2365)
                     /locus_tag="VN968_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846152.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2685..3599)
                     /locus_tag="VN968_01210"
     CDS             complement(2685..3599)
                     /locus_tag="VN968_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL27899.1"
                     /translation="MAKLPDFEGLAIFAKVVELRSFAAAATELALSKATVSKAVSRLE
                     QRLGARLFNRTSRRLALTDAGQKLSERAARLLADGEAAENEALAQSATPRGLVRFAVP
                     MTFGVSKVAPILPEFLEAYPDVAIDLHLSDAMVDLIGDGFDAGLRIASLPDSSLIARR
                     LCTMPRYTVASPAYLKRHGRPTHPMHLARHKCFGYAYLSTPDVWHYTNAAGEQASVRP
                     AGPLRVNNGEALMPALLAGLGIADLPDFIVGDAVASGEVEVILKGWKQAEGAVHLVTP
                     PGGPRPARVEVLGDFLTKHFAKGKRRTS"
     gene            3723..4421
                     /locus_tag="VN968_01215"
     CDS             3723..4421
                     /locus_tag="VN968_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXL27900.1"
                     /translation="MIELRPFAKLGGADHGWLKAKHHFSFGSHYDADNMGHGSLRVWN
                     DDEIAPNSGFPAHPHANMEIITYVREGAITHQDSLGNKGRTEAGDVQVMSAGSGVRHS
                     EYNLEPTKTKIFQIWIEPTTEGGQPTWGAKPFPKSDRSGNFVTIASGFDADKDALPIR
                     ADARVLATTLKAGESAEYTSGKTRNLYLVPAAGAVEVNGVRVNARDGVAIRDEAKLKI
                     TALEDSELVLVDSA"
     gene            4573..5160
                     /gene="wrbA"
                     /locus_tag="VN968_01220"
     CDS             4573..5160
                     /gene="wrbA"
                     /locus_tag="VN968_01220"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HXL27901.1"
                     /translation="MTKVLVLYYSAYGHIEKMASAIAEGAREAGVTVDVKRVPELVPP
                     DVAYKLDQTAPVAKIEELANYDAIIVGTGTRFGRMASQMANFLDQAGGLWMKGALHGK
                     VGGAFTSTATQHGGQETTLFSIITNLLHFGMAVVGLNYGFAGQMKLDEVTGGSPYGAT
                     TITGGDGSRQPTENELAGARYQGRVIAETAKKLHG"
     gene            5230..5361
                     /locus_tag="VN968_01225"
     CDS             5230..5361
                     /locus_tag="VN968_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27902.1"
                     /translation="MIEMLFIYQLARNPVQAVARRWWCRSLYRASRGKRRCRECTKS"
     gene            5466..5642
                     /locus_tag="VN968_01230"
     CDS             5466..5642
                     /locus_tag="VN968_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27903.1"
                     /translation="MTNRPDPKGTSKNPAEPHPGMMGDGTEEQNLGQPGNPDARIKKD
                     EVDAAFGKDAKKTK"
     gene            5745..6695
                     /locus_tag="VN968_01235"
     CDS             5745..6695
                     /locus_tag="VN968_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HXL27904.1"
                     /translation="MSDAAQAPWPELPTAAWRETHETLHLWTQIIGKIRLARAPWLNH
                     SWHVALYVTARGLTTSPIPDGTRTFQIDFDFIDHALRISTSDGVQRQLPLAGQSVASF
                     YAAVMADLADLGIHIAIDEMPNELPEPTRFSQDRKNASYDPTAVRRLLQILVNADRVF
                     KQFRTGFLGKASPVHFFWGSFDLAVTRFSGRHAPRHPGGVPHLSDAVACEAYSHEESS
                     AGFWPGSGAIDYPAFYSYAYPEPPGFRATRVRPDAAFFSEALGEFILPYDAVRMAAEP
                     DQVLLEFLQSTYEAAADAAKWDRDALECAPGRPGVVRRIL"
     gene            complement(6697..7884)
                     /locus_tag="VN968_01240"
     CDS             complement(6697..7884)
                     /locus_tag="VN968_01240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL27905.1"
                     /translation="MTILTAGAREPEARKVSRAPRYRIEFVADWKQAAARFSDLCPPT
                     PFQHPQWLDAWYGAFAGTGDVEPLIAIVSDAATGEPIALLPLIRRPQKGVRIVEFADL
                     SLTDYNAPMLTAAAPRTARTARALWRDLTAALRRMPGGADLIRLQKMPADLDGKPNPL
                     ALLDGAGPCSLNGNVVTTGDDFDAYRYSLSRTVRKELERSCRVFMRYPAATFKIVADR
                     NEALRILSTMEVQQGRRMQQLGLHFILNDEACAAFYRKLVGSGVDNGYVVVSALTVGE
                     EVVATLLGIRTGPHYLMIRISNAGEKWSNCSPGRLIIERTMAALHKQGMREFDFSIVN
                     YAYKRRFGVARLPLVDISAALSWRGLPHALRDRAARELRRYPRLAPYVRRALGRPPSR
                     EEN"
     gene            complement(7901..8839)
                     /locus_tag="VN968_01245"
     CDS             complement(7901..8839)
                     /locus_tag="VN968_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27906.1"
                     /translation="MTTSKIFSHWDETHSQLWNRQPIRLEHEMHKSPAFSMDDLARLI
                     EGYPRQHYSIVQTGVRGASRVWREGDLGNLSGREVIDAISRGELWLNLREVGSVDGRY
                     RELVDRMFEEVAARVPGFEVPEFHQAGILISSPRAEVYYHADLPWQALVQISGRKRVY
                     VYPNTAPFITPRHLEDITLFNVEVDLPYQDWYDNHAKVLDIGPGQMLNWPLNAPHRVE
                     NLDTFSVSMTISYTDDEIRRSAVINFANGMLRHRFGYQPKSRNLRGPSYFAKRVMGKL
                     LRNSSWIKRERSMRAPIDFRLDAAQPGKIVDASKAA"
     gene            complement(9327..9929)
                     /locus_tag="VN968_01250"
     CDS             complement(9327..9929)
                     /locus_tag="VN968_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HXL27907.1"
                     /translation="MKRLSTLAAGLLIGATALQYSNLASGQSEGGWITLLDSTKMGDW
                     NEVGKANWAMKDGALTVDKLDGKDLSYLVTKNSYKDFQIRAEFWTDEEANSGVFLRCD
                     QSQKIDAKICYEVNIYDKRPDPSYGTGAIVDVAKVDPMPKAAGKWNTYEITAQGPHLV
                     IVLNGQKTADVQDSKHTSGPIALQYGSGVVKFRKVQIKEL"
     gene            10139..10609
                     /locus_tag="VN968_01255"
     CDS             10139..10609
                     /locus_tag="VN968_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL27908.1"
                     /translation="MKATSRFWRYLLMPICFAGMLNISSAAELNPAAVIYKLPDQIPW
                     SPVDARGAQNAVVVGDPTKPGFYAVYTKWTKGNHFSRPHFHPNDRYIVVLQGTWWVGS
                     GPKFDPEKGTTPMPAGSFVTHFGKQVHWDGAKDEDAVLLIMGEGPATSTAAEEK"
BASE COUNT         2105 a         3390 c         3234 g         1988 t
ORIGIN      
        1 tgccgacgac ggctacgacg acttccaggc gcagatggct gaatatttca agtcgccgaa
       61 caagcatccg cccacggtgc cggaaaccgc ggcctattac cgtgccaaga agatcgtggc
      121 ggtgatcttc ccggtcgatg ccgagcgcga aaccggcttc cgccgctaca gcaatgacga
      181 gatgctggaa gtcgcggctg acaattccga tgtgttgatc ccgttcgtca gcatcgatcc
      241 gcacaagggc aagctcggcg ttcgcgaggc gcgcaggctg atcgaggaat acggcgtcaa
      301 gggtttcaaa ttccatccga ccatgcaggg cttttatccg aacgaccgca tggcctatcc
      361 gctgtatgaa gccatcaacg acggcggcgc gatcgcgctg ttccacaccg gccagaccgg
      421 ggtcggctcc ggcatgccgg gcgggatggg gatgcggctg aaatattcca acccgatgta
      481 catggacgat gtggcggcgg atttccccga cctcaagatc attctcgccc acccgtcgtt
      541 tccctggcag gaagaggcgc tgtcggtcgc gacccacaag ccgaacgtcc atatcgacct
      601 gtccggctgg tcgccgaaat attttccgcc gatcctggtg cgctacatca actcgatcct
      661 gcaggacaag atgctgttcg gctcggactg gccggtgatc acgcccgacc gctggctttc
      721 ggattttgca aagctcgaga tccgcgacga gatcaggccg aaggtgttga aggccaacgc
      781 gcgcaggctt ttgaacatct agccgatcgg aagagtattc ccgtgagcat caaagccgtt
      841 gtgttcgacg cctatggcac gctgtacgac attcagtcgg tcgccgccgt taccgaacag
      901 gcgtttcccg gctatggtga gatcatcacc caggtctggc gtatcaagca gctcgaatac
      961 acctggctgc gctcgctgat gcggcgctat caggatttct cggtcatcac gcgcgactcg
     1021 ctgtcgtaca cgctgcgatg cctcggcctg aaacacgacc ccgccgtgtt cgagcggatc
     1081 atggaaaaat atctgcacct cgacctctat ccggatgcga tcgcagcgct ggcggcgcta
     1141 cgcggtcgaa agcttgcgat cctttccaac ggcagcaccg gaatgctcga tgcggtggtc
     1201 cgcaacagcg gtctcgaccg cgtgctcgac gccaccatca gcatcgactc ccaaagaatc
     1261 ttcaaaccca gcccggatgc ctacgccttg atcgaatcca ggctcggtgt gccacctgcg
     1321 gaggtgctgt tcgtgtcgtc gaacccgtgg gacgcctgcg gcgccaaggc gttcgggctg
     1381 aatgtcgcct ggatcgagcg ggtgacgccg gaagcgatgg cgctggcctg cgtcaaaagc
     1441 gatgttgtcg ccccgctcac gatgttcaag gcgatccgga cccagatgga cgagctcgag
     1501 ctcgagcccg actatcgcat ccacgcgctg tcagaactgc ccgatctggt cgctgcgcat
     1561 gagccttgaa tccgttcgcg cgttttttgc cgataaggcg cccgacatcg ccgtgatcga
     1621 atcgccgatc agttccgcca cggtggcgct ggccgcggaa gcctatggcg tcgagcccgg
     1681 acgaatcgcc aagacgctga gcttgcggat cggcgagcgc gtggtgttga tcatcgccag
     1741 cggcacctcg cggatggaca acaagaagct caaggcgcgc ttcggcggca agccgaagat
     1801 gctcggcgtc gacgaggtcg cggacatcac cggccatgaa gtcggcggcg tctgcccgtt
     1861 cggattgaaa acgccgctgc cggtctattg cgacgtgtcg ctgaaagtgt tcgacgaggt
     1921 ggtgccggcc gccggctcga cccacagcgc ggtgcggatc gcgccgtcgc ggatggcgga
     1981 actggtcgcc gccgaatggg tcgatgtctg tcaggagccg gcgtaatgaa ccggtctatt
     2041 ggtcgtcatc gtagggcctc ggccgattgt cgcgacgtct cggttgatcg tcgcgccgtc
     2101 tcggcgcagc cggctggttc gcgctgacat tcggttcaaa caccgcgcgg cacggcccgg
     2161 agagctgcgg ggtgttttgc cgcaggcacg ccacgatccg atcgacgtcc ggtatctgtt
     2221 cgccgcagag ccgaaacaca tcgggcgtgc aggccatctg ctgttccatt gttccccgat
     2281 tctcctgcga aaaagcggga tgttgaacgg cgatgccgca gccgatcgat atcgcaaggc
     2341 tcaggacaaa caggcgggct cgcatttatc aatccttctt ctggggattc atttcccgtc
     2401 catgtgccgc acgggcgcgg cgcgcgaagc atctcgcctc aagcttgaaa ataaatgagg
     2461 ctcgggaaag ttctcaccgc acaacaaaac gtgaaagtgg cgcttttagg aaggaaacgt
     2521 gagaaaaacg tcggcgcgct ttcgccgctc cagaggcgct caatctcctc gaacagaaga
     2581 ttgctcggcc gctcggcaag aacgaccgtg aacgtaccgt ggcatgacgc atcatatcat
     2641 gagctattgt ggtgggcacg gcgcaaagag cgcctttgtc cacgctacga agttcttcgc
     2701 ttccccttcg caaaatgctt ggtcagaaaa tctcccagca cctcgacgcg cgcgggccgc
     2761 gggccgcccg gcggcgtcac cagatgcacc gcgccctcgg cctgcttcca gcctttcagg
     2821 atcacctcga cctcgcccga cgcgacggcg tcgccgacga tgaaatccgg cagatcggcg
     2881 atgccgagcc cggcaagcaa cgccggcatc agcgcctcgc cattgttgac ccgcagcggg
     2941 cccgcggggc gcacgctggc ctgttcgccc gcggcgttgg tgtagtgcca gacatccggc
     3001 gtggaaagat acgcatagcc gaagcatttg tgccgggcga gatgcatcgg atgcgtcggc
     3061 cggccgtgcc gcttgagata cgccggcgac gccaccgtat agcgcggcat cgtgcatagc
     3121 cggcgcgcaa ttaacgagga atccggcagg ctcgcgatcc gcaagccggc atcgaagccg
     3181 tccccgatca gatccaccat ggcatcgctc aagtgcagat cgatcgcgac gtcgggatac
     3241 gcctcgagaa attccggcag gatcggcgcc actttgctca ccccgaacgt catcggcacc
     3301 gcgaagcgca ccagcccgcg cggcgtcgcc gattgcgcca gcgcctcgtt ctcggccgcc
     3361 tcgccatcgg cgagcaggcg cgccgcacgc tcggacaact tttgcccggc atcggtcaag
     3421 gcaagccgcc gcgaggtgcg gttgaacagc cgtgccccga gccgttgctc cagtcggctg
     3481 acggccttgg agaccgtcgc cttggacagc gccagttcgg tcgcggccgc cgcaaacgac
     3541 cgtaattcca cgacttttgc gaaaatcgcg aggccttcga aatccgggag ttttgccatt
     3601 tgaacgttcc attagagcta ttttggaaac aattagtttc aatagtttct atttatgcac
     3661 catacggaag cgcatatcca aacaccaacg aaatttgaaa tccaacgatc aaaggaaatc
     3721 ccatgattga actcaggcct tttgcaaaac tcggcggcgc cgatcacggc tggctgaagg
     3781 caaaacatca cttctcgttc ggcagccact atgacgccga caatatgggc cacggctccc
     3841 tgcgggtatg gaacgacgac gagatcgcgc cgaactccgg ctttcccgcg catccccatg
     3901 ccaacatgga aatcatcacc tatgtccgcg agggcgcgat cacccatcag gatagtctcg
     3961 gcaacaaggg gcgcaccgaa gccggcgacg tgcaggtgat gagcgccgga tccggcgtgc
     4021 gtcactccga atacaatctg gagccgacca agaccaaaat cttccagatc tggatcgagc
     4081 cgaccaccga aggcggccag ccgacctggg gcgcaaaacc gtttccgaag tcggatcgct
     4141 ccggcaactt cgtcaccatc gccagcggct tcgacgccga caaggatgcg ctgccgatcc
     4201 gcgccgacgc gcgggtgctc gccaccacgc tgaaggccgg cgagagcgcg gaatatacgt
     4261 ccggcaagac ccgcaacctc tacctggttc cggcagcggg tgccgtcgaa gtcaatggcg
     4321 tgcgcgtcaa tgcccgcgac ggcgtcgcga tccgcgacga agccaagctg aaaatcaccg
     4381 cgctggaaga ttccgaactg gtgctggtcg actcggcgta aggccgttct cactcgtcat
     4441 gcccgcttga cccgggcatc catccctctt cggaagagtc ttgcgaagaa gatggattgc
     4501 ctggtcaagc ccggcaacga cgcctcaaat aaatcaaaaa cacctcaatc ccaaccccct
     4561 cggagaccca ccatgacaaa agtcctcgtt ctctattatt ccgcttacgg ccacatcgag
     4621 aagatggcga gcgccatcgc cgagggcgcc cgcgaagccg gcgtcaccgt cgacgtcaag
     4681 cgggtgcccg aactggtgcc gccggatgtc gcctacaaac tcgatcagac agcccccgtg
     4741 gccaaaatcg aagagctcgc caattacgac gccatcatcg tcggcaccgg cacccgcttt
     4801 ggccgcatgg catcgcagat ggcgaatttc ctcgatcagg ccggcggcct ctggatgaaa
     4861 ggcgcgctgc acggcaaggt cggcggcgcc ttcacctcga ccgcgaccca gcacggcggc
     4921 caggaaacca cgctgttctc gatcatcacc aacctgctgc atttcggcat ggccgtcgtc
     4981 ggcctgaact acggctttgc cggccagatg aagctggacg aggtcaccgg cggatcgccc
     5041 tatggcgcga ccacgatcac cggcggcgat ggtagccgcc agccaaccga gaacgagctg
     5101 gcgggcgcgc gctatcaagg acgcgtgatc gcggagaccg cgaaaaaact gcatggctga
     5161 tgcgacgcgg gcggcattct catcggcgga tgccgcccca ttttggttgt ggggacgaag
     5221 gaattcctca tgatcgaaat gctctttatc tatcaactgg cgcgcaatcc ggttcaggcg
     5281 gttgcgcgac gctggtggtg tcgcagcctc taccgcgcct cgcgcggcaa gcgccgctgc
     5341 cgggaatgca ccaagtcgtg aagccggagc actcgtccaa gtggtaaact gtgagatgga
     5401 accggatggc attcacgccg ttaggtgcgg ttaggttacg cgcaaacgcg cctggagatt
     5461 tcgaaatgac caacaggcca gatcccaaag gtaccagcaa aaaccccgcc gagccgcatc
     5521 ccggcatgat gggcgacggc accgaagagc aaaatctcgg ccagccggga aatcccgacg
     5581 cccgcatcaa aaaagacgaa gtcgacgccg cctttggcaa ggacgccaag aagacaaaat
     5641 aacgcgcatc ccaatcgctg taagttcgca tataaaggcc gggccggatc gcgcccggcc
     5701 ttgtcgtgtc aggagatgcc gatgataacc ggttgcggtg aaccatgagc gatgccgcgc
     5761 aggcgccctg gccggaattg cctactgcgg cctggcggga aacccacgaa acgctgcatc
     5821 tgtggaccca gatcatcggc aagatccgcc tggcgcgcgc gccctggctc aatcattctt
     5881 ggcatgtcgc gctgtacgtc accgcacgcg ggctgaccac ctcgccgatc cccgatggga
     5941 cacggacctt ccagatcgat ttcgacttca tcgatcatgc cttgcggatc tccaccagcg
     6001 acggcgtcca gcggcaactg ccattggccg gacaatcggt ggcaagcttc tacgccgccg
     6061 tcatggctga ccttgcagac ctcggcattc acatcgccat cgacgagatg cccaacgaat
     6121 tgccggagcc tactcggttt tcgcaagaca ggaaaaatgc atcatacgat ccgaccgcgg
     6181 tgcggcgcct tctgcagatt ctcgtgaatg ccgaccgcgt cttcaagcaa ttccgcaccg
     6241 gcttcctcgg caaggcaagc ccggtgcatt tcttctgggg cagcttcgat cttgcggtca
     6301 cacgcttttc cggccggcac gcgccgcgcc atccgggcgg cgtgccgcat ctatccgatg
     6361 cggtggcctg cgaagcctat tcccacgagg aaagcagcgc cggcttctgg ccgggcagcg
     6421 gcgcgatcga ttatcccgcg ttctattcct atgcctaccc ggagccgccg ggttttcggg
     6481 ccacgcgggt gcggccggat gcggcgttct tcagcgaggc gctcggcgaa ttcatcctgc
     6541 cctatgacgc cgtgcggatg gccgcagagc cggaccaagt gttgctcgaa tttctgcaaa
     6601 gcacctatga agccgcggcc gatgccgcca aatgggaccg tgacgcgctg gaatgcgcgc
     6661 cgggccggcc gggcgtggtg cggcggatcc tgtaattcaa ttctcttcgc gtgacggcgg
     6721 tctgccgagc gcgcgtctca catagggggc cagtcgcggg tagcggcgaa gctcgcgcgc
     6781 ggcccgatcg cgcagcgcat gggggagtcc gcgccagctc aaggccgccg agatatcgac
     6841 caaaggcagc cgcgccacgc cgaaccgccg cttgtaggcg tagtttacaa tgctgaaatc
     6901 gaactctcgc atgccctgct tgtgcagggc cgccatggtg cgttcgatga tcaacctgcc
     6961 cggcgaacag ttcgaccact tctcgccggc gttgctgatg cgtatcatca ggtagtgcgg
     7021 gccagtgcgg atcccaagca gggtcgcgac cacttcctca cccaccgtca atgccgacac
     7081 cacgacgtaa ccgttgtcaa caccgctgcc gaccagcttg cggtagaacg cggcgcaggc
     7141 ctcgtcattg agaatgaaat gcagcccaag ttgctgcatc cgcctgccct gctgaacctc
     7201 catggtcgag agtattcgca acgcttcgtt tctgtcggca acaatcttga acgtcgctgc
     7261 gggatagcgc atgaatacgc ggcagcttcg ctccagctcc ttgcgcacgg ttcggctcag
     7321 cgaataacgg tacgcgtcga aatcgtcgcc ggtcgtgacg acgttgccgt tcagcgaaca
     7381 cgggcctgcg ccatcgagca gcgccagcgg attgggtttg ccgtcgagat cggccggcat
     7441 cttctgcagg cggatcagat cagccccacc gggcatgcgc cgcagcgcgg ccgttagatc
     7501 gcgccacagg gcgcgcgccg tcctggcagt gcgcggcgcc gccgctgtca acatcggggc
     7561 attatagtcg gtcaggctga gatccgcgaa ttcgacgatc cggacgccct tttgcgggcg
     7621 gcggatcagc ggcaacagcg cgatgggctc gccggtcgcc gcatccgaga cgatggcaat
     7681 cagcggttcg acatcgcccg tgccggcaaa cgcgccgtac cacgcatcga gccattgagg
     7741 atgctggaaa ggcgtcggcg ggcagagatc actgaagcgc gcggcggcct gcttccagtc
     7801 agccacgaac tcgatgcgat agcgcggcgc gcgggaaact ttccgcgcct ccggttcccg
     7861 cgcccccgcc gtcaatattg tcatcgccgg ccttacgtgc tcatgccgcc ttcgacgcgt
     7921 cgacgatctt gccgggttgc gcggcatcga ggcggaagtc gatcggggcg cgcatgctgc
     7981 gctcgcgttt gatccagctg ctgttgcgca gcaatttccc catcacccgt ttagcgaagt
     8041 acgagggacc gcgcaaattc cggctcttcg gttgatagcc gaagcgatgg cgcagcatgc
     8101 cgttggcgaa gttgatgacc gcggagcgac gaatttcgtc atcggtatag gagatggtca
     8161 tcgagacgct gaaggtgtcc agattctcga cccgatgtgg cgcgttgagc ggccagttca
     8221 gcatctggcc aggcccgatg tcgagtacct tggcatggtt gtcgtaccaa tcctgatacg
     8281 gaagatcgac ctcgacattg aagagcgtga tgtcttcgag atgccttggc gtaatgaacg
     8341 gcgcggtgtt cgggtagaca tatacacgct tgcgtccgga aatctggacc agggcctgcc
     8401 agggcaggtc ggcgtggtaa tagacctccg cgcgcggcga cgagatcagg atgcctgcct
     8461 gatgaaattc cggcacctcg aagccgggca ccctggcggc gacttcctcg aacatgcggt
     8521 cgaccagttc acgatagcgg ccatcgaccg agccgacttc gcgcaggttg agccagagtt
     8581 cgccgcggga gatcgcgtcg atgacctccc ggccactgag attgccgagg tcgccctcgc
     8641 gccacacgcg gctcgcgcca cggacgccgg tctgcacaat gctgtagtgc tggcgcggat
     8701 aaccctcgat cagcctggcg agatcgtcca tcgagaacgc cggcgacttg tgcatttcat
     8761 gttcaagacg gatcggctgg cgattccaca gttgggaatg cgtctcgtcc cagtggctga
     8821 agatcttgct cgtcgtcatg gtcgcgctcc tctcgacttt tcgtttttgt tgacccgacg
     8881 acaaccatcc cggaacgctc caatcgcgcc ccgaaaacct gatgccgccg tcccgcggat
     8941 caccggctgt cccgatacga gacggccggc cgaatctcat ctgcttgtca gcaagaaagg
     9001 tgccgcaagg cgcgggcggc tgcttatggt gagataatgt cggggatgta tggctaacgc
     9061 aaataccttc gaaaaggccg ccgcgccgta caatgatgtc agaacctgcg caaccggtac
     9121 tctttctcgg cattgcacga cgttaacccc gacgcggtcg gaccggtcgc cgacattggc
     9181 gcacgctgtt atttgctgct tgagagccaa cgctcggtta ccgatcggac tcaacgatgc
     9241 tcaaccggcg cgagcgcagg attcgcgcgg gatggtgaac gcataccgtg ccccggatgc
     9301 agcgcatccg gggacacgag gtggctctac agctccttga tctgcacctt gcggaacttg
     9361 accacgccgg agccgtattg cagcgcgatc gggccgcttg tgtgcttgct gtcctgcacg
     9421 tcggcggtct tttgaccgtt cagcacgatc accagatgcg ggccctgcgc ggtgatttca
     9481 taggtattcc atttaccggc cgccttcggc atcggatcga ccttggcgac atcgacaatc
     9541 gccccggttc cgtaagatgg atccggccgc ttgtcataga tattgacctc gtagcagatc
     9601 ttggcatcga tcttctgcga ctggtcgcag cggaggaaca cgccgctgtt ggcttcttcg
     9661 tcggtccaga actcggccct gatctggaaa tccttgtagg aattcttggt gacgagatag
     9721 gacaggtcct tgccgtcgag cttgtcgacg gtgagcgcgc cgtccttcat cgcccaattg
     9781 gctttgccga cctcattcca gtcgcccatc ttggtgctgt cgagcagcgt gatccagccg
     9841 ccttcgctct ggccggaggc cagatttgaa tattgcaagg cggtggcgcc aatcagcaga
     9901 ccagccgcga gcgttgataa gcgcttcatg aagatgtcct cccgtttgtt ttagttgggc
     9961 gcaacgttag caccgccgtg cagggcgtaa acgcgttttt catgctgccg ttgcgttgtg
    10021 tatggtctcg catgcccgcc cgcgtcttgc accccgggaa tgagaccggc tagcatgaac
    10081 ccaatgagag atgagctggc accagcgatt cagctcgcgg gattcattgg aggaaatcat
    10141 gaaagccaca agccggtttt ggcgatacct gttgatgccg atatgctttg ccggcatgct
    10201 gaacatcagt tcggcggccg aactcaatcc ggccgcggtg atctacaagc tgccggacca
    10261 gattccgtgg agcccggtcg acgctcgcgg cgcgcaaaac gccgtcgtgg taggcgaccc
    10321 gaccaagccc ggcttctatg ccgtctacac caaatggacc aagggcaacc atttcagccg
    10381 gccgcacttc catcccaacg accgttacat cgtcgtgctg cagggcactt ggtgggtcgg
    10441 ctcggggcca aaattcgatc ccgaaaaggg cactacaccc atgcctgccg gaagttttgt
    10501 cacgcatttc ggcaagcagg tgcattggga cggcgccaag gacgaggacg ccgtgctctt
    10561 gatcatgggc gaagggccgg cgacctctac cgcggcggag gaaaaataac gacaagacct
    10621 gcgcaggagc acgagtggtg atccgttcta gccggctcag ccttcggaat tggcaggaat
    10681 cggacagaga cacatttgcc gccctcaacg ccactcc
//
LOCUS       DATNOI010000035         7227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2493397,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000035 DATNOI010000000
VERSION     DATNOI010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493397"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..1795
                     /gene="thrC"
                     /locus_tag="VN968_01260"
     CDS             377..1795
                     /gene="thrC"
                     /locus_tag="VN968_01260"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXL27909.1"
                     /translation="MTHYVSTRGEAPALGFCDVMLTGLARDGGLYVPETWPQLSRETI
                     AGFFGRPYWEVAVDVIRPFIGDEIADADLGRMANEAYATFRHPAVVPLRQIGPHQFLL
                     ELFHGPTLAFKDVAMQLISRLMDHVLAQRARRTTIVVATSGDTGGAAVDAFAGLDNVD
                     LIVLFPHGRISDVQRRMMTTSGAANVHALAIEGTFDDCQAIVKGLFNHHDFRDEVSLS
                     GVNSINWARIVAQVVYYFTSAVALGAPERTVGFTVPTGNFGDIFAGFVAKRMGLPIRC
                     LRIAANINDILPRTLKTGIYEVREVHATASPSMDIQVSSNFERLLFEASRRDAGTVRR
                     LMASLKQSGRFVLPDAVLAAIREEFDAGRADETETSAAIRAAWREAGDLVDPHTAVAL
                     AVADRDTSDSRIPNIVLSTAHAAKFPDAVEAACGVRPGLPAWLEDLTTRSEHIKVMNN
                     DQVEVERFVRSVSRAAKQGVAG"
     gene            1792..3081
                     /locus_tag="VN968_01265"
     CDS             1792..3081
                     /locus_tag="VN968_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXL27910.1"
                     /translation="MSVDVTKLPSGLTVITDSMPHLETAALGVWAGVGGRDEKPSEHG
                     ISHLLEHMAFKGTARRTSREIVEEIEAVGGDLNAGTSTETTAYYARVLKADVPLALDV
                     LSDILANPSFVPDELEREKSVIVQEIGAAQDTPDDVVFEHLNELCFPDQPMGRSLLGT
                     AKTLKRFDRDMLRGYLATHYRGPDMVVAAAGAVDHKRVVEEATRRFASFDAAPAPKPQ
                     GAVFGKGGSRVVHRELEQAHLTLALEGVPQTDPSLFSLQVFTNTLGGGMSSRLFQEVR
                     EKRGLCYSIYAFHAPYADTGFFGLYTGTDPGDAPEMMEVIVDVINDAVETLTEAEVAR
                     AKAQMKAGLLMALESCTSRAEQLARHVLAYGRPLTVEELVARIDAVSVESTRNAARAL
                     LSRSHPAVVALGSGRGLDTAVAFAEGLTRPKVKARLH"
     gene            3230..3817
                     /locus_tag="VN968_01270"
     CDS             3230..3817
                     /locus_tag="VN968_01270"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HXL27911.1"
                     /translation="MALFRLPSSGPAALAPRGHGLLLRTPQMSDFLQWAHLRESSREY
                     LTPWEPIWPSDDLTRAGFRRRLRRYTEDIAADRSYPFIVFRESDGAMIGGITLANVRR
                     GIVQAGTIGYWVGEPHAHRGYMTNALRVLLPSLFGELNLHRIEAACIPSNTPSIRVLE
                     KCGFSREGLARRYLCINGVWQDHLLFGLLHEDFRG"
     gene            4021..4617
                     /locus_tag="VN968_01275"
     CDS             4021..4617
                     /locus_tag="VN968_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27912.1"
                     /translation="MLAAPASSQSLGDRFKSLFGVRPEQPAEAAPPAEGETDLTCPPV
                     SIRAGASTYAVAAPGKQPVGNDLRVQATITRTARDCTKNGDQITARIGIQGRVIAGPA
                     GAPSTVEIPLRVAVVQGGVQEKTIATKVYRTTVSMSEDGNVPFALVAEDLVYPVPLGA
                     AGDSYIFYIGFDPQALKPEPKPKAAKKAESKKAQKPPE"
     gene            complement(4652..5137)
                     /locus_tag="VN968_01280"
     CDS             complement(4652..5137)
                     /locus_tag="VN968_01280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313570.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HXL27913.1"
                     /translation="MLFELEFWVAVAFVILMGLFAYLGVHRTVLQTLDRRSERIKAEL
                     DDARRLKEEAAKVLAEYEARRASAEREAEDIIASARAEAERIAGEAKAKMEDFVARRT
                     KTAESKIALAEAQALADVRAAAADAAVAAASTILSQSVKGEMADELLAKGIAEVRAKL
                     N"
     gene            complement(5143..5706)
                     /locus_tag="VN968_01285"
     CDS             complement(5143..5706)
                     /locus_tag="VN968_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HXL27914.1"
                     /translation="MAESRGEAKGQKTGAHSEADGGHGGAFPPFDSSTFASQLVSLVI
                     AFVALYLIVSRIALPRVGSMLDERQAAIEGDLAEAQKLKDESDAELKAYENELAAARA
                     RAHAISAETREKLNAASEAERRTLEERLTHKLIEAEKTIAQTREAAMRNVRGIAADAA
                     AAIVQRLTGVQPDGKTTKRAVDASLKG"
     gene            complement(5765..5995)
                     /locus_tag="VN968_01290"
     CDS             complement(5765..5995)
                     /locus_tag="VN968_01290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HXL27915.1"
                     /translation="MDPAAAKLIGAGIACIGMGGAGVGVGVIFGNYLAAAVRNPSAAQ
                     GQFGNLIFGFAVTEALGIFSLLIALLLLFVPL"
     gene            complement(6050..6805)
                     /locus_tag="VN968_01295"
     CDS             complement(6050..6805)
                     /locus_tag="VN968_01295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HXL27916.1"
                     /translation="MKIDPIHQFNIEPLFTIGHIGNQTIAITNSSLYMFLAVALISIL
                     MIGGVAGRQLVPGRFQSMAEISYEFVASMIRSTAGAEGMKFFPLVFSLFMFICVSNLV
                     GIIPYTFTVSSHIIITATLALLVFFIVLIYGLYRNGLKFFKIFVPSGIPIYILPLVVF
                     IEIFSFFLKPISHSVRLFANMLAGHIALKVFAGFVAMLGFSLGAIGWIGGIAPLALTV
                     ALTALELLVAFLQAYVFAILTCIYLNDAIHPGH"
     gene            complement(6878..>7227)
                     /locus_tag="VN968_01300"
     CDS             complement(6878..>7227)
                     /locus_tag="VN968_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HXL27917.1"
                     /translation="GGNGSRDKSSDEAALSARLGSLDHRLSEIRDNRKIRTDQPGSGS
                     GDAAARASAMARGFRLSSELIAGVVVGAVIGWGFDRLLSTSPFGLIVFFLLGFVAGVV
                     NVVRSAGVTPGRR"
BASE COUNT         1309 a         2235 c         2362 g         1321 t
ORIGIN      
        1 aagtaagccg taaaaccatt gcgcggggaa tgtcgggtga atccgtctga acctgtggtg
       61 actaactcgt gtgctttatc acttcgcacg cgaggctgcg ggtgcagttg gcacccggca
      121 ttccctgcgc cctctgttat cgagggacat tgtctggcaa agctcgggcg cacattgcgt
      181 cgcgagatcg cggacttgca tccgaaactg gcgcggaaag ataaagcgcc gcgcaccatg
      241 atctttttcg ccgtagcggc atttcccatt gtcccgaaaa cgctttatgg tgccgcgcgg
      301 cttttgccat cggcagaact gaattatcaa ccaatatcaa agacttagga tgaagcccga
      361 gcctttggag ggcggtttga cgcactacgt ttcgacgcgg ggggaggccc cggccctagg
      421 cttttgcgac gtgatgctga ccgggctcgc ccgcgacggc ggcctctacg tgcctgaaac
      481 ctggccgcag ctttcacgcg aaaccatcgc cggatttttc ggccggccat attgggaagt
      541 ggcggtcgat gtgatccgcc cgtttatcgg cgacgagatt gccgatgccg atctcggccg
      601 catggccaat gaggcctacg ccaccttccg ccaccccgcg gtggttccgc tgcgccagat
      661 cggtccgcat caattcctgc tcgagctgtt tcacgggccc accctggcct tcaaggacgt
      721 ggcgatgcag ctgatctcgc ggctgatgga ccatgtgctg gcgcagcgcg ccaggcgcac
      781 cactatcgtg gtcgcaacat caggcgatac cggcggcgcc gcggtcgatg cctttgcagg
      841 tctcgataat gtcgatctga tcgtgctgtt tccgcacggc cggatttccg acgtgcagcg
      901 gcggatgatg acgacgagcg gcgcggccaa cgttcacgcg ctcgccatcg aaggcacttt
      961 tgacgattgc caggcgatcg tgaaggggct gttcaatcat cacgatttcc gcgacgaggt
     1021 ctcgctgtca ggcgtcaatt cgatcaattg ggcgcggatc gtggcccagg tggtgtacta
     1081 ttttacgtcg gcggtcgcgc tcggcgcgcc ggagcggacg gtaggcttca ccgtgccgac
     1141 cggaaatttc ggcgacatct ttgcgggctt cgtcgccaag cggatggggc tgccgatccg
     1201 gtgcttgcgg atcgccgcca atatcaacga catcctgccg cgcacgctca aaaccggcat
     1261 ctatgaagtg cgcgaagttc acgccaccgc gtcaccgtcg atggatattc aggtctcgtc
     1321 gaatttcgag cggctgttgt tcgaggcgag ccgccgcgat gccggcaccg tccgccggct
     1381 gatggcgtcg ctgaaacagt ccggacgctt cgtgctgccc gacgcggtgc tggcggcgat
     1441 ccgcgaggaa ttcgatgccg ggcgggccga tgagaccgaa accagtgcgg cgatccgcgc
     1501 ggcctggcgc gaggccggcg atctcgtcga tccgcacact gcggtggcgc tggcggtggc
     1561 ggatcgcgac acctcggatt cgaggattcc caacatcgtg ctgtcgaccg cgcatgccgc
     1621 gaaatttccc gatgcggtag aggccgcctg cggggtgcgc cccgggttgc cggcctggct
     1681 cgaggacctg acgaccaggt ccgagcacat caaagtcatg aacaacgatc aggtcgaagt
     1741 cgaacgattc gtgcgctcgg tcagccgtgc cgcgaagcaa ggagttgccg gatgagcgtc
     1801 gatgtcacca agcttccctc cggcctcacc gtcatcaccg acagcatgcc gcatctggag
     1861 accgcggcgc tcggggtgtg ggccggcgtc ggcggccgcg acgaaaagcc gagcgagcac
     1921 ggcatttcgc atttgctcga acacatggcg ttcaagggca ccgcgcggcg cacctcgcgc
     1981 gaaatcgtcg aggagatcga ggcggtcggc ggcgacctca acgccggcac ctcgaccgag
     2041 accaccgcct attatgcgcg ggtgctgaag gccgatgtgc cgctggcact tgacgtgctc
     2101 tccgacattc tcgccaatcc atccttcgtg ccggacgagc tcgagcgcga aaagagcgtc
     2161 atcgtgcagg agatcggcgc ggcgcaggat acgcccgacg acgtggtgtt cgaacatctt
     2221 aacgagcttt gttttcccga tcagccgatg gggcgctcgt tgctggggac ggcaaagacg
     2281 ctgaagagat ttgaccgcga catgctgcgc ggctatctcg ccacccatta ccgcggtccg
     2341 gacatggtgg tggcggcggc cggcgcggtc gatcacaagc gcgtcgtcga ggaagcgacg
     2401 cggcggttcg caagcttcga tgctgcgccg gcaccgaagc cgcaaggcgc ggtgttcggc
     2461 aagggcggct cccgcgtggt gcatcgcgag ctcgaacagg cgcatctgac cctggccctt
     2521 gagggggtgc cgcaaaccga cccctcgctg ttctccttgc aggtcttcac caacacgctc
     2581 ggcggcggca tgtcgtcgcg gctgttccag gaagtgcgtg agaagcgcgg gctgtgttac
     2641 tcgatctacg cctttcatgc gccctacgcc gacaccggct tcttcgggct ctataccggc
     2701 accgacccgg gcgatgcgcc cgaaatgatg gaggtgatcg tcgatgtcat caacgacgcc
     2761 gtcgagacgt tgaccgaggc cgaggttgcg cgcgccaagg cgcagatgaa ggccgggctg
     2821 ttgatggcgc tggaaagctg cacctcgcgc gccgaacagc tggcccgtca cgtgctggcc
     2881 tacgggcgcc cgctgacggt cgaagagctg gtcgcccgga tcgatgcggt gagcgtggaa
     2941 tcgacccgga acgcggcacg ggcgctgctg tcgcgcagcc atcccgcggt cgtcgccctt
     3001 ggcagcggaa ggggtctgga cacggcggtg gcttttgcgg aaggattgac ccggccgaag
     3061 gtcaaggcgc gccttcattg agggctgcgg cgtactgatt atcgcatgat ccggaaaagt
     3121 aggaaccggt tctccgaaaa gatcatgcgc aaacaaagag atgagatcat gatgcgattt
     3181 ggcttaatcg caacatgttc tagcgttggg gcatcaggga gtactgcaca tggccctgtt
     3241 tcgtttgcca tccagcggac cggcggcgct tgccccgcgc ggccatggcc tcttgctacg
     3301 cacgccgcag atgtccgatt ttctgcaatg ggcgcatttg cgcgaatcca gccgggaata
     3361 cctgacgccg tgggagccga tctggccgtc ggacgacctg acccgcgccg gcttccggcg
     3421 ccggctgcgc cgctataccg aggacatcgc cgccgacaga tcctatccgt tcatcgtgtt
     3481 tcgcgaatcg gacggcgcga tgatcggcgg cattacgctc gcgaacgtcc ggcgcggcat
     3541 cgtgcaggcc ggcaccatcg ggtactgggt cggagaaccg catgcgcatc gcggctacat
     3601 gacgaacgcg ctgcgcgtat tgctgccctc gctgttcggc gagctcaatc ttcaccgcat
     3661 cgaggccgcc tgcatcccct ccaatacccc ctcgatccgg gtgctggaga aatgcggctt
     3721 cagccgcgaa ggcctggcgc ggcgctatct ctgcatcaac ggcgtctggc aggaccatct
     3781 gctgtttggc ctgctgcatg aggattttcg cggctgacac cgggttttgc tgcgcctttg
     3841 tgcgccttgg gttcgccgcc attgcgctgc tataacgcgg ccccaagggg gacgagggtc
     3901 ggatggggat gccagggacg tcgaatgggt gacaagcatg tgatcatgag ggctgccgcg
     3961 tctggcagct gtcgcaggat cgcgcttctg gccgcggtct catttgcagc cggttacggc
     4021 atgttggcgg cgccggcgtc ctcgcagtcg cttggcgatc gcttcaagag tctctttggc
     4081 gtaaggccgg aacaacccgc cgaggcggcg ccgcctgccg aaggcgagac cgacttgacc
     4141 tgtcctccgg tcagcattcg cgccggcgcg tcgacctatg cggtggccgc gccgggcaag
     4201 cagccggtcg gcaacgatct gcgggttcag gccaccatca cgcgaaccgc ccgcgattgc
     4261 acgaagaacg gcgaccagat caccgcacgg atcggcattc agggccgcgt catcgccggc
     4321 ccggcgggcg cgccttccac ggtcgaaatc ccgcttcgcg tcgcggtggt gcagggcggc
     4381 gtgcaggaga agacgatcgc caccaaggtc taccggacga cggtatcgat gtcggaggac
     4441 ggcaacgtcc cgtttgccct ggtcgccgag gacttggtct atccggtgcc gctcggcgcg
     4501 gccggggatt cctacatctt ctatatcggc ttcgatccgc aggcgctgaa accggagccc
     4561 aagccgaaag cggcgaagaa ggcggagagc aagaaggcac aaaagccgcc ggaatgatcg
     4621 cgggcggctt ttgtcttggg gaaggaaggc ctcagttcag cttggcgcgg acttcggcga
     4681 tgcccttggc caaaagctcg tcggccatct cgcccttcac cgattgcgac aggatggtgg
     4741 aagcggcagc gaccgcggcg tcggcagcgg ccgcgcggac atcggccagc gcctgggctt
     4801 cggccagcgc gatcttgctc tcggcggtct tggtgcgccg cgcgacgaaa tcttccatct
     4861 tggccttggc ttcgccggca atgcgctcgg cttcggctct tgcgctggca atgatgtcct
     4921 cggcctcgcg ttcggcgctg gcgcgacgcg cctcatattc cgccagcacc ttagcggcct
     4981 cttccttgag gcggcgcgca tcgtcgagtt cggccttgat gcgctcgctg cgacgatcga
     5041 gcgtctgcag cacggtgcga tggacgccca gataagcgaa caggcccatc aggatgacga
     5101 aggctacggc gacccagaat tccagttcga acaacatcgc aactatccct tcaaggaggc
     5161 gtcaacggcg cgctttgtcg tcttgccgtc gggctgcacc ccggtgagcc gctgcacgat
     5221 cgcggcggcg gcgtcggcgg cgatgccgcg gacgttgcgc atcgcggcct cgcgggtttg
     5281 ggcgatggtt ttctccgcct cgatgagctt gtgcgtcaaa cgctcttcca gcgtcctgcg
     5341 ctcggcctcg gatgccgcgt tgagcttttc gcgggtctcg gcgctgatcg cgtgcgcgcg
     5401 ggcgcgcgcc gcagccagtt cgttttcata ggctttcagc tcagcgtcgg actcgtcctt
     5461 cagcttctgc gcctcggcca gatcgccctc gatcgctgcc tgacgctcgt caagcatgct
     5521 gccgacgcgc ggcagcgcga ttcgcgagac aatcaggtaa agcgcgacga aggcgatgac
     5581 cagcgacacc agctgggaag cgaaggtgga ggagtcgaac ggagggaatg ctccgccgtg
     5641 accgccatcg gcttcgctgt gagcgccggt cttctggcct tttgcctcgc cacgactctc
     5701 agccatggag ttctcctgtt gctgtcgtgc gcgccgtctt aaaagacgac gcgagggaac
     5761 agccttagag cggaacgaac agcaggagca gcgcgatcag cagcgagaag atgccgagcg
     5821 cttcggtcac ggcgaagccg aagatcaggt tgccgaactg gccctgggcg gcggatggat
     5881 tgcgcaccgc ggcggcgagg tagttgccga agatgacgcc tacgcccacg cccgcaccgc
     5941 ccatgccgat gcacgcgatg cccgcgccga tgagttttgc tgctgccgga tccattttta
     6001 gctccctgag gaaagaaaga cgaagggtgg gtggaattcc cggtaccgtt taatgtcccg
     6061 gatgaatggc gtcgttgaga tagatgcagg tcaggatcgc aaacacatag gcctgcagga
     6121 acgcgaccaa gagttcgagc gcggtgagcg ccacggtgag cgcgagcggg gcgatgccgc
     6181 cgatccagcc gatggcgccg agcgagaaac cgagcatggc gacgaagccc gcgaacacct
     6241 tgagcgcgat atggccggcc agcatgttgg cgaacagacg cacgctgtgc gagatcggct
     6301 tcaggaagaa cgagaagatc tcgatgaaga cgacgagcgg caggatgtag atcggaatgc
     6361 ccgacggcac gaagatcttg aagaatttca ggccgttcct gtagaggccg taaatcagca
     6421 cgatgaagaa caccagcagt gcgagcgtcg ccgtgatgat gatgtggctc gagacggtga
     6481 aggtgtaggg aatgatgccg accaggttcg agacgcagat gaacatgaac agcgagaaga
     6541 ccagcgggaa gaacttcatg ccctccgcgc cggcggtgct gcggatcatg gaggcgacga
     6601 actcgtagct gatttcggcc atcgactgga atcgcccggg caccagctgc cgccccgcaa
     6661 cgccgccgat catcaggatc gagatcagcg ctaccgccag gaacatgtag agcgacgagt
     6721 tggtgatggc gatcgtctga ttgccgatat ggccgatcgt gaacaacggc tcgatgttga
     6781 actggtggat cgggtcgatt ttcatccgcg cggctcttgg tcctccggct ttcctgccgg
     6841 caatcgaatt tcaaaggcca ggacttcccg tcagcgctca gcgcctgccc ggggtcacgc
     6901 ccgcagacct caccacgttg accacgccgg cgacaaagcc gagcagaaag aacacgatga
     6961 gaccgaacgg cgatgtcgac aacaagcggt cgaatcccca gccaatcacc gctccgacaa
     7021 caacgccggc gatcaactcc gaagaaagcc ggaaaccgcg tgccatcgcc gaggctctgg
     7081 cagctgcgtc tccacttcca gatccgggtt ggtcagtcct gattttgcgg ttgtcccgaa
     7141 tttcggacaa acggtgatcc agacttccga gccttgcgga aagcgcagct tcgtcggacg
     7201 atttatcgcg acttccattt ccgccgt
//
LOCUS       DATNOI010000036        11815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_858276,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000036 DATNOI010000000
VERSION     DATNOI010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11815
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858276"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VN968_01305"
     CDS             complement(<1..823)
                     /locus_tag="VN968_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL27918.1"
                     /translation="MAEKFSKLEAGADAADVPLLRIDAVAKKFGGIRAVDRLSLDIRA
                     GEFFALLGPSGCGKTTLLRMLAGFETPDEGRILLNGHDIAQYLPHERPVNMMFQNYAL
                     FPHLTVWDNIAFGLKRAAMARAGIERRVTALVALVKLQGMEKRKPDQLSGGQKQRVAL
                     ARSLARQPRVLLLDEPLAALDKKLRESTQLELMELQRRLGMTFIIVTHDQEEAMTMAD
                     RIGVMDAGRLEQVATPRELYEAPISRWVAEFVGDVNLYEGQVGAREAGRLAISTRD"
     gene            981..2306
                     /locus_tag="VN968_01310"
     CDS             981..2306
                     /locus_tag="VN968_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL27919.1"
                     /translation="MNRDRYDLPVTTKSDRAAAFYRDGVDRMLSAWHGAANAFDAAIA
                     EDPAFAIAHIARARIHQMNMEVTEARAEAALARQFAATATQRERRHVEIMASVIEGQP
                     RAALSGAEQHLEEFPRDAPVLSLLLGAFGLYAFSGRADHDAARVAICERHARHYGEDW
                     WFLTYRGWSHTEAGNPALGRAATERAIMLRPENANAAHGLSHAQFEQGDLAEGRTFLS
                     SWLQAHDRASFLHGHLSWHVALAALEAGDLDGALAIYQQHIRPAGAPYPPLNVFTDCA
                     SLLWRLSLAGETGLAPYWQEVAAYGDRFFPQAGAHFADIHYALVAAATGSGVLATRLT
                     QMETRDADGKLAPGSSAIGLCRGAQAFAEGDNENAIRILEPLMPAVVRIGGSHAQREL
                     WEDTLIVAYLRAGHRDKAMRMISDRLRRRPSARDEAWSRQALQGTGRRL"
     gene            2613..3488
                     /locus_tag="VN968_01315"
     CDS             2613..3488
                     /locus_tag="VN968_01315"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL27920.1"
                     /translation="MVVPFPAGGPTDIVARPLAQMLGEALKAIVLVDNRGGAGGSLGA
                     DAVAKSPPDGQTLLMATVGTHAINPALYRNLSYDAESDFTPVALVALAPVAVVVHPSQ
                     PMTNLAELVDLAKHMPGKLDYGSAGVGTPGHLTAEMFKAAAAIDIQHIPYKGSAPAVT
                     DLLGGQIQIMFDPLQSVLSNVLAGKLRALAISSKARSPVLPDVPTIAESGYNGFETTA
                     WWGVFAPARMPDGLTATIAGEVERIVNSEAFRRQLEPLGVIPTVLSRDDFSKFQHSEL
                     AKWARAVHDGGIRID"
     gene            3494..4732
                     /locus_tag="VN968_01320"
     CDS             3494..4732
                     /locus_tag="VN968_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HXL27921.1"
                     /translation="MKLNDEESDILAGKAGPVPQQALQHQIKVGEFFGAEDMVPVTQA
                     HLMADTESLGEAGVRWLEGLAATAGGQRRVRIPTITDPRGTDFAKADLLGQTSAMLSL
                     ERRAISAFEALGVAMTDTCINYQTIQAPTRNEHVAFGDTGVVIYSNSICGARSNFEGG
                     PSALAAGLTGRTPRYGFHLPEMRRATLRIQVEFTPTSLNGWGALGGVIGRIGGNYWAV
                     PVIEGVEGAPKADQLKHFGASMASFGSIALFHLAGLTPEAGKLTDVAAPGLPSHRVTK
                     ADVDQLQRSYHTDDAVDVVVFSAPQLSLFELSDLAELCEGRRFKVPLLAVTSPQVKPD
                     ADRMGYTARIEAAGGCVLSGMCFYQSYAREISEARGWKRLATNSAKLVNILGGYGYRP
                     MLASMEACVDAAVSGKLART"
     gene            4729..5139
                     /locus_tag="VN968_01325"
     CDS             4729..5139
                     /locus_tag="VN968_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HXL27922.1"
                     /translation="MNNKTFRARHAMGSTVRGPALVANDGLSARYDLDRINGIFCRPS
                     HKLAGQSYVGKILVLDTAKGGVATAWMLHEMKSRGIVPLALVLNSVNPILAQGAALGE
                     VPMLAGFDEDITAAVPNGAEVEVNPEAGVLTIVG"
     gene            complement(5152..6624)
                     /locus_tag="VN968_01330"
     CDS             complement(5152..6624)
                     /locus_tag="VN968_01330"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HXL27923.1"
                     /translation="MADYDLAIIGGGLNGVSVARDAAGRGLRVILLEQGDLGAGASSA
                     SPRLIHGDPALLERRAFLRVRAALAERDIWLRIAPHLVRPMRFAIPAHSDERPSWLLR
                     SGLLLYDRLASPAALPASATIDVTHHPVGNALKRPFGTAFEYSDCVVDDSRLVVANAV
                     DAAERGAVIRTGARCVRADRLDVWRLAVIDRGHRQVITARALANATGAWTASVAETVL
                     RMLPPHVGTAQISQIIVRRLFDTDSVYVFQNSDRRLIFASPYERDFTLIGTVGRAFKG
                     DPAIVSVPAGDIAYLCDAANRYFRERIEASDVIRTVSGANAVMDPQSRRPERDGSIIF
                     DARRGKAPLLTIFGGDVTTSRLRAERTVSRLTPFYPMSPRWTAKTPLPGGDFDWTRFD
                     DEVDGARERWRFLSEAQAQRLVAAYGSRLEAVLGNAKDRAELGPAFGPELTAAEVRYL
                     MTKEWARFPEDILWRRSKLGLTMPPPDREALAVFMATAGA"
     gene            complement(6714..9407)
                     /locus_tag="VN968_01335"
     CDS             complement(6714..9407)
                     /locus_tag="VN968_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXL27924.1"
                     /translation="MAKKNSRAGGKIVIAAQAIACAIATIAPLGAVWGASTDFAPKSY
                     LGTADPNMVWEILMAGVVVSSFLAAVVLWVHSAFRKVKRLQSRRNAFVSSALNQLSHG
                     AVMTDAQNRIVFCNDRYLEIYGLARSDISRNMTGPELLEMRRERGVLDVSVGDFYAQA
                     GTPEGLITELPSGRSVLVKYFALPNGGSVATHEDYSEQRKLSRELASTKQFLESVLDN
                     VPVCVAAKSIEDGHYIFANRAFERFSRFSRDIIVGSRADAIFTTATAAKIEAADQAAL
                     NSPDGQFRSEFVVERGSEKRVLASNRVVARNEKNEPEFLIALFDDVTDRKSLSEELES
                     TKKFLELVVDNIPVSLTVERASDRRYLLANRSAETILNRRREDATGLTAADIFNPKEA
                     KLIIARDEAAIRKHGLLTEEHPISTKDGLRLFLTRRMTVLDDAGEPQYLIKTHEDVTD
                     RRQTESRMAHMAYHDGLTDLPNRAAFMQALSQMIEACEGTDEEFAVLCVDLDGLKEVN
                     DVFGHATGDKLLIEVAHLLQDLARDGVVARLSGDEFGLIIDGTQPTAGVALAEQLAEA
                     LANEFHIDGKAVRTGVTTGISVFPQNGADAASLLVNAGAALFRAKTASRGSISVYEPE
                     MDRQIRDRRVLHQDLSVAIRNGELSLHYQPQAMSRQTAASSEVIGFEALARWNHPVRG
                     FVPPGDFIPLAEESGLIVEMGEWILREACREAASWPMPLQIAVNLSPAQFMHGDMVSL
                     LHSILLETGLAPGRLELEITEGVLIEDFDRGLALLRRLKALGIRISMDDFGSGYSSLS
                     YLQAFPFDKIKVDRAFVMNLGRNHQSAAIVRAVIGLGHGLEMSIVAEGVETQEQLSFL
                     SEEGCDAVQGYFIGRPQPIGQYAALVGRSSGNTVEAARKTG"
     gene            9671..10774
                     /locus_tag="VN968_01340"
     CDS             9671..10774
                     /locus_tag="VN968_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL27925.1"
                     /translation="MDKAECVVIGAGVIGLAVARRLAQAGREVVVLEAAEGIGTVTSS
                     RNSEVIHAGIYYPAGSLMARMCISGKRALYQYCSNHGVPHRNCGKLIVATTANETAKL
                     QSIRAHAEANGVLDLQTLSGDAARALEPALNCDAALLSPSTGIIDSHAFMLALRGDAE
                     DAGAAFAFHTPLLHARAAGERIELDAGGSAPISLECRLLVNAAGLGAAAVARGIDGMP
                     IDLIPRAYLAKGNYFSCTARAPFSHLIYPVPEPGGLGVHLTLDMAGQARFGPDVEWVD
                     TIDYAVDPARAARFYPAIRRYWPMLPDGALMPSYSGIRPKIVPPAVAVQDFLIQGPRD
                     HGVDGLINLFGIESPGLTSSLAIADHVGELAGV"
     gene            complement(10874..11059)
                     /locus_tag="VN968_01345"
     CDS             complement(10874..11059)
                     /locus_tag="VN968_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HXL27926.1"
                     /translation="MAIQAHLVELERKHKSLENELHEALVHLSTDDLQIVELKRRKLM
                     VKDQIERLKHTAEETLH"
     gene            11315..11521
                     /locus_tag="VN968_01350"
     CDS             11315..11521
                     /locus_tag="VN968_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HXL27927.1"
                     /translation="MTDEDERELESELARLQQEHRDLDAAIDALHQSPAPDLLRLQRL
                     KKRKLQLRDRIAFIEDQITPDIIA"
     gene            complement(11652..>11815)
                     /locus_tag="VN968_01355"
     CDS             complement(11652..>11815)
                     /locus_tag="VN968_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL27928.1"
                     /translation="RAVRGRWPPIKIVATSGRVEIAETDLPEGGRFLPKPYGPTDVAG
                     VLREVTGGG"
BASE COUNT         2207 a         3732 c         3708 g         2168 t
ORIGIN      
        1 catcccgcgt cgagatcgcc agccgccccg cttcgcgcgc gccgacctgg ccctcataca
       61 gattgacgtc accgacgaac tccgcgaccc agcgagaaat gggcgcctcg tagagctcgc
      121 gcggcgtcgc gacctgttcg agacggccgg catccatcac gccgatccgg tccgccatcg
      181 tcatcgcctc ttcctgatca tgggtgacga tgatgaaggt catgccgagc cggcgctgca
      241 gctccatcag ttcgagctgg gtgctttcgc gcagcttctt gtcgagcgcg gccagcggct
      301 cgtcgagcag cagcacccgc ggctggcgcg ccagcgagcg ggccagcgcg acgcgctgtt
      361 tttggccgcc ggagagttga tcgggctttc gtttctccat gccctggagt ttcaccagcg
      421 cgaccaatgc ggtcacacgc cgttcgatcc cggcacgggc catggccgcg cgcttcaatc
      481 cgaacgcgat attgtcccac accgtcagat gcggaaacag cgcgtagttc tggaacatca
      541 tgttgacggg acgctcgtgc ggcaggtatt gcgcgatgtc gtggccattc aagagaatgc
      601 ggccctcgtc cggcgtctca aaaccggcga gcatccgcag aagcgtggtc ttgccgcagc
      661 cgcttggacc gagcagagcg aaaaactccc ctgccctgat gtcgagcgac agccggtcca
      721 ccgcacggat gccgccgaat tttttcgcca ccgcgtcgat gcgcaaaagg ggaacgtcgg
      781 ccgcgtctgc gcccgcttcg agtttcgaaa atttctcagc catattcctc gccagcccat
      841 tcgcggctgc acgctagcgg cggatcggca ccggctcaac cacggcacgg cgcgcagcca
      901 caatattatc gccagaaacc tccacaatcc ggtggtttca tggtgcagta tggtggttac
      961 ctgcttggca gggaggcaag atgaaccggg acaggtacga tctgccggta acgacgaagt
     1021 cggaccgcgc ggcggcattt tatcgcgacg gcgtcgaccg catgctttcg gcatggcacg
     1081 gtgccgccaa cgcctttgat gccgcaatcg ctgaagatcc ggcctttgcc atcgctcaca
     1141 ttgcgcgcgc tcgcatccat cagatgaaca tggaagtcac ggaagcgcgc gccgaggcgg
     1201 cgctggcgcg gcaatttgcg gcgacagcaa cccagcgcga acgccggcac gtcgagatca
     1261 tggcttcagt catcgagggg cagcccagag ccgcgttgag cggcgccgag cagcaccttg
     1321 aagagtttcc gcgcgatgcg ccggtgcttt cgcttttgct tggtgctttc ggcctctatg
     1381 cattttccgg ccgcgccgac cacgatgcgg cgagggtcgc gatctgcgag cgtcatgcca
     1441 ggcattacgg cgaggattgg tggttcctca cctatcgggg ttggtcgcac accgaggcag
     1501 gcaatcccgc tctcggtcgg gcagcgacgg agcgggccat catgctgcgg cctgaaaacg
     1561 caaatgcggc gcacggtctc tcccacgccc aattcgagca aggcgacctg gcggaaggcc
     1621 gcacgtttct ttcctcctgg ttgcaagcgc acgaccgggc gagcttcctg cacggacacc
     1681 tttcgtggca tgtcgcgctc gcggcactag aggccggcga tctggacgga gcgcttgcga
     1741 tctaccaaca gcacatcagg ccggcgggcg cgccatatcc gcccttgaac gtgttcacgg
     1801 attgcgcttc gctgctctgg cggctctcgc ttgccggcga gacaggtctg gcgccctatt
     1861 ggcaggaagt cgccgcctat ggcgatcggt tcttcccgca ggcgggcgca cactttgccg
     1921 atatccacta cgcgctggtc gcagcggcaa cgggcagcgg ggttctggca accagactga
     1981 cgcaaatgga aactcgtgac gccgacggca aactcgcccc aggttcgtcc gcgatcggcc
     2041 tttgccgcgg ggcgcaagca ttcgccgaag gcgacaacga aaacgcgatc cggattctcg
     2101 agccgctgat gcccgcggtt gtgcggatcg gcggcagcca tgcccagcgc gaactgtggg
     2161 aggacaccct gatcgtggcc tatctgcgcg ccggacaccg cgacaaggcg atgaggatga
     2221 tctccgaccg tctgcggcgc aggccatcgg cccgtgacga ggcatggtcg cggcaagctc
     2281 tgcagggaac agggaggcgg ctttgaagac accaggaaag cagcgcccga tcacaatact
     2341 gcctggattt atactgcctg gatttcgtct gcgctttttc ggcgcggcga ccgaagagcc
     2401 acaaagattg cctcgaacgt ttccaggctc ccgttgagtg agaacagccg ttacagttga
     2461 ctccgagtca aaagaattgg cagcctgaac ggcgggggac gcttatgcag cgcagacaat
     2521 tcctgagaat tgctgcggct gcggctggcg ccagcctgct gacgcgagcc gggcccgcga
     2581 gggcgcaatc gcttgccggc aagcaaatcc gtatggttgt accgtttccg gctggcgggc
     2641 caaccgacat cgtggcccgc ccccttgcgc aaatgttggg ggaggcgctc aaagcgattg
     2701 tgctggtaga caatcgcggc ggcgcaggtg gttcgttggg agccgacgcc gtcgccaagt
     2761 cgccccccga tggccaaacg ctattgatgg cgacggtggg cacgcacgcc atcaaccccg
     2821 ctttgtacag gaacttgtcc tacgatgccg aaagcgactt cacgcctgtc gcgctggtgg
     2881 cgctggctcc ggtggccgtc gtcgtgcacc cctcgcagcc gatgacaaat ctcgccgagc
     2941 tggttgatct ggccaagcac atgcctggca agctggacta cggctcggcc ggcgtcggca
     3001 cgcccgggca cctgaccgcc gagatgttca aggccgcagc agctatcgat attcagcaca
     3061 ttccctacaa gggcagcgcg cccgccgtga ccgacctgtt gggcggccag atacaaatca
     3121 tgttcgatcc gttgcaatcg gtgctgtcga acgtgttggc gggcaagctg cgcgcccttg
     3181 cgatctcaag caaggcacgc tcacctgtgc tgcccgatgt ccctaccatc gccgagtccg
     3241 gatataatgg ttttgaaacg accgcatggt ggggcgtgtt tgcgccggcc aggatgccgg
     3301 acgggctgac tgccacgatc gccggcgagg ttgaacggat cgtcaacagc gaggcgttcc
     3361 gccgccagct cgagcctttg ggcgtgatcc cgacggtact cagtcgcgat gatttttcca
     3421 aatttcagca cagcgaactg gcgaaatggg ccagggcggt gcatgacggc ggcataagga
     3481 tcgattgacc tcgatgaaac tcaacgatga agagagcgac atcctcgccg gcaaggccgg
     3541 cccggttccg cagcaggcct tgcagcatca gatcaaggtc ggcgaattct tcggcgccga
     3601 agatatggtg ccggtgacgc aagcccacct gatggccgat accgaaagcc ttggcgaagc
     3661 cggcgtgcgg tggctggagg gtctcgctgc gaccgcgggt gggcagcggc gcgtccggat
     3721 tccaaccatc accgacccgc gcggcaccga cttcgccaaa gccgatttgc tcggtcagac
     3781 gtcagccatg ctcagtctcg aaaggcgggc gatttccgca ttcgaagcgc tcggtgtcgc
     3841 gatgaccgac acctgcatca actatcagac cattcaggct ccgacacgca acgagcatgt
     3901 cgccttcggc gacactggcg tcgtcatcta ttcaaactcg atatgcggcg cacgatccaa
     3961 ttttgagggc ggcccctcgg cactggcggc cggccttacc gggcgaacgc cccgctacgg
     4021 ctttcacctt ccggaaatgc gccgggccac tctgcgcatt caggtggagt tcacgccgac
     4081 atccctgaac gggtggggcg cgcttggcgg agtgatcggg cgtatcggcg gcaactattg
     4141 ggccgtgcct gtgatcgagg gcgtcgaagg agcgccgaag gcggaccagc tcaaacattt
     4201 cggcgcttcg atggcgagct tcggctcgat tgcgttgttt catctggcag ggctgacgcc
     4261 cgaagccggc aaactgactg acgtggcggc gccgggtttg ccaagccatc gcgttaccaa
     4321 ggccgacgtc gatcaattgc aaagatccta tcataccgat gatgcggtgg acgtcgtggt
     4381 cttctcggcg ccgcagctca gcctttttga attgtccgac ttggccgaac tgtgcgaagg
     4441 ccggcgcttc aaggtgccgc tgctggcggt caccagcccg caggtaaaac cagacgctga
     4501 ccgcatgggc tacacggcgc gaatcgaagc agccggcggc tgcgtcctgt ccggcatgtg
     4561 cttctatcaa tcctatgcgc gagagatttc cgaggccagg ggctggaagc gcctggccac
     4621 caacagtgca aagctggtca atattctggg cgggtacggt taccggccga tgctggcgtc
     4681 gatggaggca tgtgtggatg ccgcagtcag cggaaaactg gcacgaacat gaacaacaag
     4741 acttttcgcg cgcgacatgc catgggaagt accgtacgcg ggcccgcgct ggtcgccaat
     4801 gacggccttt cggctcgcta cgacctcgac cgcatcaacg gcatcttctg tcgtccctcg
     4861 cacaagctgg cgggccagtc ctacgtcgga aaaatattgg tgctggacac ggccaaaggc
     4921 ggggtcgcca ccgcctggat gctgcacgag atgaagtcgc gcgggatagt gccactggcg
     4981 ctggtcctca attccgtgaa tcccattcta gcgcaaggtg ccgcgctggg agaagtaccg
     5041 atgctcgccg ggtttgacga agacatcacc gccgctgttc ccaacggtgc cgaagttgaa
     5101 gtgaaccccg aagcgggcgt gctgacgata gtgggttagc gaattcccta ctcaggcccc
     5161 cgccgtcgcc atgaacaccg ccagcgcctc gcggtcaggc ggcggcatcg tcaagcccag
     5221 tttcgaccgc cgccacagaa tgtcctccgg aaaccgcgcc cattcttttg tcatgaggta
     5281 gcgaacctcg gcggccgtca gttccggccc aaaggccgga ccgagttcgg cccggtcttt
     5341 ggcgtttcca agcacggcct caaggcgcga gccataggcg gcgaccagcc gttgagcctg
     5401 agcctcgctc agaaaccgcc agcgctcgcg cgcgccatcg acctcatcgt cgaaccgcgt
     5461 ccagtcgaaa tcaccgcccg ggagcggcgt cttcgcggtc cagcgcggcg acatcggata
     5521 gaaaggggtg agcctggata cagtccgctc cgcgcgcagc cgcgaggttg tgacgtcacc
     5581 gccgaagatc gtcagcagcg gcgccttgcc gcgccgagca tcgaaaatta tcgagccatc
     5641 gcgctcgggg cgcctgcttt gcgggtccat gaccgcgttg gcgccggaaa ccgtccggat
     5701 cacatccgat gcttcgatcc gctcccgaaa ataccggttg gccgcgtcac agagataggc
     5761 gatatcgccg gccggcaccg agacgatcgc gggatcgccc ttgaacgcgc gccccacggt
     5821 gccgataagg gtgaaatcgc gctcataggg gctcgcaaag atcagccgcc ggtcgctgtt
     5881 ctggaacaca tagacactgt cggtatcgaa caggcgccgg acgataatct ggctgatctg
     5941 ggcggtgccg acgtgcggcg gcagcatgcg caacacggtt tcggcgaccg aggcggtcca
     6001 ggcgccggtg gcattggcca aggcccgcgc cgtgatcacc tggcgatggc cgcgatcgat
     6061 cacggccagt cgccagacat cgagccggtc ggcgcgaacg caacgcgcgc cggtgcggat
     6121 caccgcgccc cgctccgcgg catcgacggc attggcgacc accagccgcg aatcgtccac
     6181 cacgcaatcg gaatattcaa acgcggtgcc gaatggccgc ttcagcgcgt tgccgacggg
     6241 atggtgcgtg acgtcgatcg tcgccgatgc cgggagcgcc gcgggcgagg ccagccggtc
     6301 gtaaagcagc agccccgagc gcagtaacca tgacggacgc tcgtcggaat gcgcgggaat
     6361 cgcaaagcgc atcgggcgta ccagatgcgg cgcgatccgc agccagatgt cgcgttcggc
     6421 aagcgcggcg cgcacgcgca ggaaggctct gcgctccagc agggccggat cgccatggat
     6481 cagccgcggc gaggccgagg atgcgcccgc gccgaggtcg ccctgttcca gcaggatcac
     6541 ccgcaggccg cgtccggcgg cgtcgcgcgc cacgctgacg ccgttcaagc cgccgccgat
     6601 gatcgcaaga tcgtagtccg ccatgcgcgg cccatagcgt tttcgagcga agtggatacc
     6661 ggttcgggtg aagaaaacgc gtcaaaaaag gaggttcggc tctgaaccga accctagccg
     6721 gtcttgcgcg cggcctccac cgtgttgccg ctgctgcggc cgaccagcgc ggcgtactgg
     6781 ccgatcggtt gaggtctgcc aataaaatag ccctgcaccg cgtcgcagcc ctcctccgag
     6841 aggaaggaga gttgctcctg ggtttccacg ccctcggcga cgatcgacat ctccaacccg
     6901 tggccaaggc cgatcacggc ccgaacgatc gccgccgatt ggtgattgcg gccgagattc
     6961 atgacgaaag cgcggtcgac cttgatcttg tcgaatggaa acgcctgaag gtagctcagc
     7021 gaggaatagc cgctgccgaa atcatccatg gaaatgcgaa tgccgagcgc cttcaaccgc
     7081 cgcagcagcg ccagaccgcg atcgaaatcc tcgatcagca cgccttcagt gatttccagc
     7141 tcaagccggc ccggcgcgag accggtttcg agaagtatcg aatgcaacag gctgaccata
     7201 tcgccatgca tgaactgcgc cggcgacagg ttgaccgcga tctgcagcgg catcggccac
     7261 gacgcggcct cccggcaggc ttcacgcagg atccattccc ccatttcgac gatcaagccg
     7321 ctttcttccg caagtggaat gaaatcgccg ggcggcacga agccgcgcac cggatggttc
     7381 caccgcgcca gcgcctcgaa cccgatgact tcgctggagg ccgcggtctg gcgcgacatc
     7441 gcttgcggct gataatgcag cgacaattcg ccgttcctga tcgcgaccga gagatcctga
     7501 tgcaacacgc ggcgatcgcg aatctgccga tccatttccg gctcatagac gctgatcgag
     7561 ccgcgcgatg ccgtcttggc gcggaacagt gccgcgccgg cgttgacgag caacgacgcg
     7621 gcgtcggcgc cgttctgcgg aaataccgat atgccggttg tgaccccggt gcgcaccgct
     7681 ttgccgtcga tgtggaactc gttggccagc gcttcggcaa gctgttcggc cagcgccacg
     7741 cccgcggtgg gttgcgtgcc gtcgatgatc aatccgaatt cgtcgccgga gagccgggcc
     7801 acgacgccgt cacgcgccag gtcctggagg aggtgcgcca cctcgatcag cagcttgtcg
     7861 ccggtcgcat ggccgaacac gtcgttgact tccttcaggc cgtcgaggtc gacgcacagc
     7921 accgcaaact cctcatcggt gccctcgcag gcctcgatca tctgcgacag cgcctgcatg
     7981 aacgcggcgc ggttcggcag atcggtcagg ccgtcgtgat aggccatatg ggccatgcgc
     8041 gattcggtct ggcggcggtc ggtgacgtcc tcgtgggtct tgatcagata ttgcggctcg
     8101 ccggcgtcgt cgagtaccgt catgcggcgg gtcaggaaca gccgcaagcc gtccttggtg
     8161 ctgatggggt gttcttcggt gagcaggcca tgcttcctga tcgccgcctc gtcgcgcgcg
     8221 atgatcagct tggcttcctt cggattgaaa atatctgcag ccgtcaatcc ggtagcgtct
     8281 tcgcgccggc ggttgaggat ggtctcggcg ctacgattgg cgagaagata tcgtctgtcg
     8341 ctggcgcgct cgacagtcag cgagactgga atattatcga ccaccagttc gagaaatttc
     8401 ttggtgcttt ccagctcctc ggaaagcgat ttgcggtcgg tgacgtcatc gaacagcgcg
     8461 atcaggaatt ccggctcgtt cttttcgttg cgcgcgacca cgcggttgga ggcgagaacg
     8521 cgcttttccg aaccgcgctc gaccacgaat tcgctgcgga actggccatc gggagaattg
     8581 agcgccgcct ggtccgccgc ttctattttt gccgccgtgg cggtcgtgaa tattgcatcg
     8641 gcacggctgc cgacgattat gtcgcgggaa aagcgcgaga agcgttcgaa cgcgcgattg
     8701 gcaaaaatat agtggccgtc ctcgatgctc ttggcggcca cgcataccgg cacgttgtcg
     8761 agcactgatt ccaggaactg cttggtcgac gccagctctc tcgaaagctt gcgctgctcg
     8821 ctgtaatcct catgcgtcgc caccgaaccg ccgttcggca atgcaaaata tttgaccaga
     8881 accgaccgcc cgctgggcag ttcggtaatg aggccttccg gtgtgccggc ttgcgcatag
     8941 aagtccccga cgctgacgtc gaggacgccg cgctcgcgcc gcatctccag cagttccggt
     9001 ccggtcatgt tcctggaaat gtccgagcgt gccaggccat agatctcgag atagcggtcg
     9061 ttgcagaaca cgatccggtt ctgtgcatcc gtcatcaccg cgccgtggct gagctggtta
     9121 agggcggagc tgacgaatgc attcctgcgt gactgtaaac gcttcacctt gcggaacgcc
     9181 gagtgaaccc acagcacgac agctgcgaga aaggagctga ctacgacgcc ggccatcagg
     9241 atttcccaaa ccatgttggg gtcggcagtg ccgagatagc ttttcggcgc aaagtcagta
     9301 gatgcgcccc agacggcccc caggggcgca atggtagcaa tcgcgcaagc gatggcctgc
     9361 gcagcaatca caatcttgcc gcctgcccgc gaattctttt tagccatcag tcacccaacg
     9421 attttcgcgt cggagtgtct ggcctcacgg gttttgatca ggtaaacgcg taccctcgca
     9481 acttgtaaat gtgtcttaaa ttatggcaat tgccctgaca tgattaattc ttccctaacg
     9541 gcaaaccagc ccggaaaacg ctgccaaata gggattttcg gcagcgcgat agtgtgcgaa
     9601 tgcgattgat cctcggcgcg ccaaattggg cgaacgcgcc tcgagctgaa tagccggatc
     9661 cgaactgcaa atggacaaag ccgagtgcgt cgttatcggt gcgggggtga tcggccttgc
     9721 cgtcgcacgg cggctggcgc aggccggccg cgaggtggtc gtcctggagg cggcggaagg
     9781 tatcggcacc gtaacatcgt cgcgcaacag cgaggtgatc cacgccggca tctattatcc
     9841 ggcggggagc ctgatggcgc ggatgtgcat cagcggcaag cgcgccctgt atcaatactg
     9901 cagcaaccat ggcgtcccgc atcgcaattg cggcaagctg atcgtcgcga cgacggcgaa
     9961 tgagaccgcg aaactgcaat cgatcagggc ccacgccgag gccaatggtg tgctcgatct
    10021 gcagactctc tctggcgatg ccgcgcgcgc attggagccg gcgctgaatt gcgacgccgc
    10081 cctgctctcg ccttccaccg gcatcatcga cagccacgcc ttcatgctgg cgctgcgcgg
    10141 cgatgccgaa gatgccggcg cagcctttgc ctttcatacg ccgttgctgc acgctagggc
    10201 ggccggtgag cgaatcgagc tcgacgccgg cggcagcgcc ccgatcagcc ttgaatgcag
    10261 gcttctcgtc aatgccgcag ggcttggcgc cgccgcggtc gcgcgcggca tcgacggcat
    10321 gccgatcgac ctgataccgc gggcctatct cgcaaaagga aactatttca gttgcaccgc
    10381 ccgggcgccg ttttcgcacc tgatctatcc ggtgccggag cccggcgggc tcggcgtgca
    10441 cctcaccctc gacatggcgg gacaggcccg gtttggtcct gacgtggaat gggttgatac
    10501 gatcgattat gccgtcgacc cggcgcgggc ggccagattc tatccggcga tccggcgata
    10561 ctggccgatg ctcccggacg gcgcactgat gccgagctat tcgggcattc ggccgaagat
    10621 cgtgccgccg gcggtggccg tgcaggattt cctgatccaa gggccgcgcg accatggcgt
    10681 cgacggactg atcaatctgt tcggaataga atcgccgggc ctgacatctt cgctggcaat
    10741 agcggaccat gtcggcgagc tggcaggggt atagcgggca aggcttcctt cgaacctccc
    10801 aaaacgcgca catccccaag ggtgagcgcg attgcgctcc tcccgggagg acggcgatgc
    10861 ttgtgaaggc tggctagtgg agtgtttctt cggcggtatg cttcaaccgc tcgatctggt
    10921 ccttaaccat caatttgcgg cgctttaact caacaatttg caggtcgtct gttgaaaggt
    10981 gtacgagtgc ttcgtgtaat tcgttctcga gactcttgtg tttccgttcc agttcaacga
    11041 gatgcgcctg aattgccata tggagcctcc tcggggattg aacctcagat ttgatccggg
    11101 cacgccagtg tacacgagcc ggccaatctg tcgatgggta tcgaaaattg taccctcagt
    11161 tttgggtatc atatgtaacg aaccgtgagc acggaaccgg ggtggttccc tgaaccgcgg
    11221 cctgcttgca gaccgcgctg gcagggacga tattcctccc acggcttgcg caaacctttc
    11281 gcagccccgt cgcgtttttc ggctaacgtg caccatgacc gacgaagatg agcgcgaact
    11341 cgaatccgag ctcgcacggc tgcagcagga gcaccgcgat ctcgacgcgg cgatcgatgc
    11401 gctgcatcaa tcgcctgcgc cggatttgtt gcggctgcaa cggctgaaaa agcgcaagct
    11461 gcaactgcgc gaccgcatcg ccttcatcga agaccagatt acgcctgaca ttattgcata
    11521 accttcgtcg tcgcgggtcg catccaacgg gtccgcgcaa agcgcggccc gatgacgggc
    11581 tccgacgcga acccggaatc caagacgggg tgctggtact tggaaccatc cctgaatgac
    11641 agtctggtgg tctaaccgcc gcctgtcact tctcgcagta cgcccgcaac atctgttgga
    11701 ccgtagggtt tgggtagaaa ccgtccgcct tcgggcaaat ccgtttccgc aatctcgacg
    11761 cggccggagg tcgcgacaat cttgatcggt ggccagcgcc ccctgaccgc ccggg
//
LOCUS       DATNOI010000037        13470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1931443,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000037 DATNOI010000000
VERSION     DATNOI010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13470
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1931443"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VN968_01360"
     CDS             <1..901
                     /locus_tag="VN968_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL27929.1"
                     /translation="FGISVIIFVLLRIVPGNIVDILFAAAGYVDPADKANLERELGLD
                     QSIVMQYLHWIGGLLRGDLGYSYVSEKPALDEILPRIPITARLAGLALLFSASIGIPL
                     GVISAVNQGTRLDYILRVVSLSGLSLPSFWLGLLILMASVALFGTMPIFNPNPQSFGE
                     ACAIYCVPALAVGFRSAALVMRITRSSMLEILRQDYIRTARAKGASERAVNYHHALKN
                     AILPVITVIGIEAAFLVGGLIVTETVFNIPGVARFLVEAIRWRDYPIVQNLVMLIALV
                     VVVANFTVDMLYAAFDPRIRYTD"
     gene            910..1803
                     /locus_tag="VN968_01365"
     CDS             910..1803
                     /locus_tag="VN968_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL27930.1"
                     /translation="MDAINYDTELRRAGASATHGWRKLTFLAQRHLLGTIGLVIMVVF
                     VLAAALADGICRFSPLTVDSAHALSAPNAQHWMGTDSFGRDVWSRIIHGARISLAVGI
                     GSTALGSSIGVMIGLASGYLSGWVDLVCQRVIDILQALPLLVLALVMSAALGPSLPNT
                     IIAIAIPLIPTVARVIRANTLALRELAFVEAAKSIGMSEMRIALRHVLPNTLAPLIVL
                     ATAQLGAAILTEASLSFLGLGIPEPYPSWGRMLSESAAEYVRTAPWLVIFPGVAISLA
                     VFGTNLLGDALRDILDPRQRG"
     gene            1803..2816
                     /locus_tag="VN968_01370"
     CDS             1803..2816
                     /locus_tag="VN968_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL27931.1"
                     /translation="MSDAQTEAMVLDVKGLRTVFFTNSGLFKAVDDVSFSVCRGETLA
                     IVGESGCGKSVTALSVMRLVPDPPGRIVGGSVMLEGIDLLSLDESEMRAIRGNRISMI
                     FQEPMTSLNPVMRIGDQITEAVRLHRPMSRKEATAKAVEMLRLVKIPEAERRAQEYPH
                     QLSGGMRQRAMIAMALACRPALLIADEPTTALDVTIQAQILALILELQRELGMGLVLI
                     THDLGVVAQTAQRVIVMYAGRKVEEADVETLFASPRHPYTRGLMASIPSLPSLSTNAD
                     APLVEIPGMVPSLTNLPKGCAFAPRCGLAMARCREEYPPLQAWGENHLAACWRAAETA
                     RSP"
     gene            2813..3805
                     /locus_tag="VN968_01375"
     CDS             2813..3805
                     /locus_tag="VN968_01375"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HXL27932.1"
                     /translation="MTDMRALLEVTGLVKQYPVRSGILRRQVGTVHAVDGVSFTVGVG
                     ETLGLVGESGCGKSTVARSILRLVEPTGGSIRINGSDITNLGKAELRTHRRSMQIIFQ
                     DPFASLNPRMTAGDIVGEPLSVHGLAAGGKKQERVAELFHQVGLRPDQMKNYPHQFSG
                     GQRQRICIARALSLGPSLIVCDEPVSALDVSIQAQVINLLIDLQRKHDFSYLFIAHDL
                     AVVAHISHRVAVMYLGRIVEIAEKTELFANPRHPYTQALLASVPVADPKAKRLVPLLD
                     GDVPSPINPPSGCAFHTRCRYVMERCKAETPVLADVGSTHRVACFLNEGTGRKA"
     gene            complement(3926..5950)
                     /locus_tag="VN968_01380"
     CDS             complement(3926..5950)
                     /locus_tag="VN968_01380"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HXL27933.1"
                     /translation="MSDGPMKRTPFRTMLIANRGEIALRIMRTARRLGFGVVAVYSDA
                     DREALHVRAADQAIRIGGALPAQSYLRIEAIIAAAKAGGADAVHPGYGFLAENEDFAQ
                     ACRDAGLVFIGPSPEAIKAMGNKAGAKAIMQAAGVPCVPGYQGADQSDGGMLTEARKI
                     GFPVMIKAVGGGGGRGMRQVADAAAFPDLLRGARSEAQGAFGDPTVILERAIADPRHI
                     EIQVFGDRYGHAIHLGERDCSVQRRHQKLIEEAPSPAVSPELRARMGAIAVAAIKSIG
                     YEGAGTLEFLLDKTGEFYFMEMNTRLQVEHPVTEAITGLDLVELQLRVAAGEPLGLRQ
                     EETKFSGHAIEVRLCSEDAAHDFMPQSGRMALWQMPDELRVEHALESGSEIPPFYDSM
                     IAKLISHGATRDEARRRLIQGLEQTAAFGVTTNQAFLAACLRHPEFAAGEATTAFIGK
                     HRGDLLVRRNDVSSDAALAALLLYVTDPHAPPWRSGRTLAATFPIAARLEFDRGIQDL
                     EIARERDGGYAIQLDGREHRFEIGELGCDTIRFQTNGLMESARFFQDRDRLHVLHRGV
                     TLVVRDLTLAAPAATAATGSDGKVRAAMNGRVVAVLVKQGERVAAGQPVMTLEAMKME
                     HVHTAPISGTISAIDVAEGEQVTTGKIVVEIGSGGVTGASRDLRHSGTVR"
     gene            complement(5947..7563)
                     /locus_tag="VN968_01385"
     CDS             complement(5947..7563)
                     /locus_tag="VN968_01385"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HXL27934.1"
                     /translation="MAILESTIAPSSDAFKANRAGMLALIARMRGLEERTRAASAAAK
                     DRFHKRGQLLPRERVTLVLDPGSPFIELSTLAGYMFDVPDADKSVPGGGVIAGIGFVS
                     GVRCMVSANDSGIDAGALQPYGLDKTLRVQELALENKLPYVQLVESAGANLLRYRVED
                     FVRGGNIFRNLARLSAAGLPVVTVTHGSSTAGGAYQTGLSDYIVMVRGRTRAFLAGPP
                     LLKAATGEIATEEELGGAEMHTGISGLGDYLAEDDRDALRIARDIMANLRWDRPSPAK
                     TPHRPPQHDPEELLGIMPMDHKRPVDMRQVIARIIDDSDFTEFGANYGPATVCGHARI
                     EGEAIGIVTNNGPLDPAGANKATHFIQACCQSRTPLLYLNNTTGYMVGKAYEEAGMIK
                     HGSKMIQAVTSAGVPQITIYCGASFGAGNYGMCGRGFHPRFCFSWPNAKTAVMGGEQA
                     AETMAIVMEAAAIRRGKPVEKEKLDAMKAQIIGVFDGQMDVFSTSARVLDDGVVDPRD
                     TRGILAEVLAICREAEARNPQRMQFSVARP"
     gene            complement(7684..8550)
                     /locus_tag="VN968_01390"
     CDS             complement(7684..8550)
                     /locus_tag="VN968_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXL27935.1"
                     /translation="MSDKAVITCALNGVLTDPKQHHVPVTPEEMAREAKAAFNAGASV
                     MHIHLRQQEPDKGHLPSWEVSVSREIQQAIREGCPGVIINHTTGTSGPNYSGALDCVR
                     ETRPEMAACNAGSLNYLKVKSDNTWAWPPMMFDNAVEKVQTYLDVMKQVDTIPEFECF
                     DVGIVRCVGMYRQTGMYSGPLEYNFVMGVASGMPADPELLPILLKLKLPEAHFQVTAI
                     GRAEIWPLHQRCAEFGGHLRTGLEDTFYQPDGAKVTSNGQLIESIAACARRAGREIAS
                     PAEARQIFGIRR"
     gene            complement(8658..9479)
                     /locus_tag="VN968_01395"
     CDS             complement(8658..9479)
                     /locus_tag="VN968_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL27936.1"
                     /translation="MNDRDRRDSGRLMLISPERVFYAGLLGRPRKRSSGGFNIYTVMQ
                     GSLEITGGKSEVVGEVALVRPYVPHSVESEHPSIICLVIEPETVEPSAMNGLAARIRG
                     AEAPDIAQRIRTAYETLRRQERRGGFTTQEFDQLSFGERLPDRSMDHRIRSAALMLND
                     FSGSKMTAADCAASVHLSPSRFLHLFKDQTGISFRAFRAWKRARHLLHFVNEDINLAH
                     LAQDIGYPDSTHFSHSIRRFYGLQPRAIFSGSRDLAIYRSDPGMRDSAGFRQGRP"
     gene            complement(9659..10828)
                     /locus_tag="VN968_01400"
     CDS             complement(9659..10828)
                     /locus_tag="VN968_01400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL27937.1"
                     /translation="MRRAGTWLLAAACAIVIANNYYNQPLLADFARTFHVTEREAGGA
                     SIMTQGGYALGMLVFLPLGDMIDRRLLINALLLGSAGALVAAARAPTMQWLLVASFAV
                     GFTSAVPQVIVPFAAHLALPAERGRVVGIVMGGLLAGILLSRSIAGAIATLLGWPAMY
                     WIAGGTMVLLIPVLSVLLPKDKPVFDGNYSGLMKSLWGLVREQPVLRETSLTAALQFA
                     AFSAFWTTLAFHLNSMPEHYGSDVAGLFGLVGIAGVAAAPLAGKLADLGSPRLTVLLS
                     SLVVLAAFMVLATAGYSLAGVAAGVVLLDLGMQSGHVSNMTRNYSLKAEAMSRLNTVY
                     MVTRFVGGALGSAAGNYAWSLYQWPGVCAVGLLLALLTIAALFMLRAPSPEGVLP"
     gene            11154..12047
                     /locus_tag="VN968_01405"
     CDS             11154..12047
                     /locus_tag="VN968_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL27938.1"
                     /translation="MDRLDGMAAFAAAVEAGSLSGAAKRLGCSLASVSRQISAIERHF
                     GVTLLARTTRRLALTDEGRDYYEHVKRIVSEVVDAERTLSAQAKVPHGRLRISAPTLI
                     GRLRLAPLLSAYLADHPQVSIDLTLLDRLPNLLEDEIDLAIVLGHLPDSSLVRRSLGH
                     VRRVLCAAPDYLAIRGMPATPQDLATHDCLIFTTTPGSATWTFQISGRRTEIEVPARL
                     RSNALDVVVSAALGGSGIARAPSWQVQDHVDAGRLQLMLQDYERPSLPLQMIFSDPRH
                     FSRKVQSFADFLAQQWNGDKR"
     gene            complement(12072..12722)
                     /locus_tag="VN968_01410"
     CDS             complement(12072..12722)
                     /locus_tag="VN968_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXL27939.1"
                     /translation="MITLYHCHAARSFRPLWMLEELGLAYELKMLPFPPRVFAKEYLA
                     LNPLGTIPLMIDGETKMTESSGICHYLGTKYGPTPLMVGLDEPAYGAFLNWMYFSDAT
                     LTFPQTLVLRYSQLEPEERRNPQVATDYAKWFLGRLRAVEAATAKAETLCADRFTAAD
                     IVIGYALRLADNIALAKDFGPNVAAYWQRLQARDGYKRAVAAETLAGEQQNVARRS"
     gene            complement(12747..>13470)
                     /locus_tag="VN968_01415"
     CDS             complement(12747..>13470)
                     /locus_tag="VN968_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXL27940.1"
                     /translation="DYVINGGKMWITNGVQADWICLLANTGDAPKHRNKSLICVPMKT
                     KGVQVARKLDKMGMRSSDTAQIFFDDVRVPKRNRIGEEGHGFTYQMVQFQEERLWGAA
                     ACLKAHETIIDETIEYTRGRNAFGHSILDNQTVHFKLAEMQTEVELLRALIYRAGEAL
                     VAGEDVTRLATMAKLKAGRLGRELTDACLQFWGGMGFMNETLVSRAYRDSRLTSIGGG
                     ADEIMLTVLCKMMGTLPSLNQK"
BASE COUNT         2409 a         4366 c         4134 g         2561 t
ORIGIN      
        1 gttcgggatc tcggtcatca tctttgtgct gctgcggatc gtacccggca atatcgtcga
       61 catcctgttt gccgccgccg gatatgtcga cccggccgac aaggccaatc tggaacgcga
      121 actcgggctc gatcaatcga tcgtcatgca gtatctgcac tggatcggcg gactgctgcg
      181 cggcgatctc ggctattcct atgtatccga aaagcccgcg ctcgacgaaa tcctgccgcg
      241 aataccgatc acggcgcggc tggccggtct ggccttgctg ttctccgcgt cgatcgggat
      301 tccgcttggc gtcatcagcg cggtcaatca gggcacccgg ctcgattata tcctgcgcgt
      361 cgtgagcctg agcggccttt cgctgccatc gttctggctc ggcctgctga tcctgatggc
      421 ctcggtcgcc ctgttcggaa ccatgccgat cttcaacccc aatccgcaga gctttggcga
      481 ggcatgcgcg atctactgcg tcccggcatt ggcggtcggc ttccgcagtg ccgcgctggt
      541 gatgcggatc acgcgttcat cgatgcttga aatattgcgg caggattaca tccgcacggc
      601 gcgtgccaag ggcgcctccg aacgggccgt caactatcac catgcgctta aaaacgccat
      661 tcttcctgtc atcaccgtga tcggcatcga agcggctttt ctggtcggcg gattgatcgt
      721 caccgaaacc gtgttcaata tccccggcgt cgcccgcttt ctggtggaag cgatccgctg
      781 gcgcgattat ccgatcgtcc agaacctggt gatgctgatt gccctggtgg tcgtggtcgc
      841 caacttcacg gtcgacatgc tctacgcggc gttcgatccg cgaatccgat acacggatta
      901 ggaggtcaat tggacgcgat caactacgat accgaattga gacgcgccgg agccagcgcg
      961 acccacggct ggcgcaagct cacgttcctg gcgcagcgcc atttgctcgg caccatcggc
     1021 ctggtcatca tggtagtgtt cgtgctggcc gcggcgctgg ccgacggcat ctgccgcttc
     1081 agcccgttga ccgtggattc cgcgcacgcg ctttcggcgc ccaacgcgca gcactggatg
     1141 ggaacggact cgttcggccg cgatgtctgg agccggatca tccatggggc ccgcatatcg
     1201 ctggccgtcg gcatcggatc gaccgcgctc ggctcgtcca ttggcgtgat gatcggtctc
     1261 gcttcgggtt atctgtccgg ctgggtcgat ctggtttgcc agcgggtgat cgatattctg
     1321 caggcgctgc cgctgctcgt cctcgcgctg gtgatgtcgg ccgcattggg gccgtcgctg
     1381 ccgaacacca tcatcgccat cgcgataccg ctcattccca cggtcgcccg cgtcatcagg
     1441 gccaacaccc tggcgctgcg cgagcttgcg ttcgtggagg ccgccaagtc gatcggcatg
     1501 agcgagatgc gcatcgcgct tcggcacgtg ttgccgaaca cgctggcgcc attgatcgtg
     1561 cttgccaccg ctcaattggg tgccgccatt cttaccgaag catcgctatc gtttctcggt
     1621 cttgggattc ccgagccgta cccgtcatgg ggacgcatgc tttcggaatc ggccgccgaa
     1681 tatgttcgca ccgccccctg gctggtgatt tttcccgggg ttgcgatcag cctcgccgtg
     1741 ttcggaacga atcttctcgg cgatgcgctc cgcgacatcc tcgacccgag gcagcgcggc
     1801 tgatgagcga cgcgcaaacg gaagcgatgg ttctcgacgt gaagggtctg cggacggtgt
     1861 tcttcaccaa ttcagggctg ttcaaggcgg tcgacgacgt ttcgttcagc gtttgtcgcg
     1921 gcgagacctt ggcgatcgtc ggcgagtccg gctgcggcaa gagcgtgacg gcgctttcgg
     1981 tcatgcgcct ggtgcccgat ccgccgggtc ggatcgtcgg cggctccgtc atgctcgaag
     2041 gcatcgacct tctcagcctc gatgagagcg agatgcgcgc gatccgcggc aaccgcatct
     2101 cgatgatctt ccaggagccg atgacctcgc tcaacccggt gatgcggatc ggcgaccaga
     2161 tcaccgaggc ggtccgcctg catcggccga tgagccggaa agaggcaacg gccaaggccg
     2221 tcgaaatgct gcgcctcgtc aagatcccgg aagcggagcg gcgcgcccag gaatatcccc
     2281 atcagctctc gggcggaatg cgccagcgcg cgatgatcgc gatggcgctc gcctgccggc
     2341 cagcgctgtt gatcgccgac gagccgacca ccgcgcttga cgtcaccata caggcccaga
     2401 tcctggcttt gatcctggag ctgcagagag aactgggtat gggacttgtc ctgatcacgc
     2461 acgatcttgg cgtggtcgca cagaccgccc agcgcgtcat cgtcatgtat gccggccgga
     2521 aggtcgaaga ggctgacgtc gaaaccttgt tcgcgagccc aaggcatccc tacacccgcg
     2581 gtctgatggc ttcgattcca tccctgccct cgctcagcac caacgccgat gccccgctgg
     2641 tcgagattcc cggcatggtg ccctcattga ccaatctgcc gaaaggctgc gccttcgctc
     2701 cgagatgcgg actcgcaatg gcccgctgcc gcgaggaata tccgcccctg caggcctggg
     2761 gcgagaacca tttggcggcg tgctggcgcg cggcggaaac cgcgagatca ccatgaccga
     2821 tatgcgcgct ctgcttgagg tgacgggcct cgtcaaacag tacccggtgc gcagcggcat
     2881 ccttcgccgc caggtcggca ccgtgcatgc ggtggacggc gtcagtttca cggttggcgt
     2941 cggagaaacg ctcgggcttg tcggcgaatc cggttgcggc aaatcgacgg tcgcgcggag
     3001 catcctgcgc ctggtcgagc cgaccggcgg cagcatccgg atcaacggca gcgacatcac
     3061 caatctcggg aaagccgagc tgcgtacgca tcggcgctcg atgcagatca tcttccagga
     3121 cccgtttgcc tcgctcaatc cgcgcatgac ggctggcgat atcgtcggcg agccgctcag
     3181 cgtgcacggg ttggcggccg gcggcaaaaa gcaggaacgc gtcgccgaat tgttccacca
     3241 ggtcgggctc cgcccagacc aaatgaagaa ttatccccac cagttctccg gcgggcagcg
     3301 gcagcgcatc tgcatcgctc gcgcgctgtc gctcgggccg agcctgatcg tgtgcgacga
     3361 gccggtatcg gcgctcgacg tctcgatcca ggcccaggtg atcaatctgt tgatcgacct
     3421 gcagcgcaag cacgattttt cgtatctgtt catcgcgcat gatcttgccg tcgtggccca
     3481 tatcagccac cgcgtcgccg tgatgtatct cggccgcatt gtcgagattg cggagaagac
     3541 cgagctgttc gcaaatcccc gtcatcccta tacccaggct ttgctggctt cggttccggt
     3601 tgcggatccc aaggcaaaac gcctggtgcc gttgctcgat ggcgatgtgc cgagcccgat
     3661 caatccgccg tcaggctgcg ccttccacac ccgctgtcgt tatgtgatgg agcgatgcaa
     3721 ggcagagacg ccagtgctag ccgatgtcgg ttcgacgcat cgggtggcgt gttttctcaa
     3781 tgagggaacg gggcgaaagg cctagcttcg gtcattccgg ggcgatgcca acgggtccgc
     3841 gcgaagcgcg gcccgatgac aggctcccat cgaacccgga atctcgagat tccccgatgt
     3901 gcaattgcac atccgaggtc tggtgctaac gcaccgtccc ggaatgacgg aggtcacgac
     3961 ttgcaccggt cacgccgccg cttccgatct ccacgacaat cttccccgtc gtcacctgtt
     4021 cgccctcggc gacatcgatg gccgaaatcg tgcctgagat cggcgcggta tgcacgtgct
     4081 ccatcttcat ggcctccagc gtcatcaccg gttggccggc tgcgacgcgc tcgccctgtt
     4141 tgaccagaac cgcaacgaca cggccgttca tcgccgcgcg gaccttgccg tcgctgccgg
     4201 ttgccgccgt cgcggccggc gcggccaacg tcagatcgcg gacgacaagc gtgacgcccc
     4261 gatgcagcac atggagccgg tcgcggtcct ggaaaaatct tgccgattcc atcaggccgt
     4321 tggtttgaaa acggatcgta tcgcagccaa gctcaccgat ctcgaaccgg tgctcgcggc
     4381 catccagctg tatggcgtaa ccaccatcgc gctcgcgcgc gatttccaga tcctgaatgc
     4441 cacgatcaaa ctcaagacga gcagcgatcg gaaaggttgc cgcgagcgtc cgtccgctgc
     4501 gccacggcgg cgcatggggg tcggtaacgt aaagcagcaa cgccgcgagc gcggcatccg
     4561 aggacacgtc gttgcgtcgc accagcagat cgccgcgatg cttgccgata aaagccgtcg
     4621 tcgcttcgcc agccgcgaac tcagggtgcc gcaggcacgc cgcgagaaac gcctgattgg
     4681 tggtcacgcc gaatgcggcg gtttgctcaa ggccctgtat gagcctgcgc cgcgcctcat
     4741 cgcgggttgc gccatggctg atgagtttgg cgatcatcga atcatagaac ggcggaattt
     4801 ctgaaccgga ctccagcgcg tgctcgacgc gcagttcgtc cggcatctgc caaagtgcca
     4861 tccgtccgga ttgcggcatg aaatcgtggg cggcatcctc cgagcacagc cgcacctcga
     4921 tcgcatgccc ggagaatttt gtctcctcct gccggagacc gagcggctcg ccagcggcga
     4981 cacgcaattg cagttcaacg agatcgagcc cggtgatcgc ttcggtgacg ggatgttcga
     5041 cctgcaggcg ggtgttcatt tccatgaagt agaattcgcc ggttttatcg agcaggaatt
     5101 ccagcgtgcc ggcaccctca tatccgatcg acttgattgc cgcgaccgca atggcgccca
     5161 tccgcgcccg caattccggc gacacagccg gcgatggcgc ttcctcgatc aatttctggt
     5221 gccgccgctg caccgagcaa tcgcgctcgc cgaggtgaat cgcatggcca taccgatcgc
     5281 cgaacacctg gatttcgatg tgtcggggat cggcaatcgc gcgctccagt atcacggtcg
     5341 gatcgccgaa cgcgccttgc gcctcggacc gcgcgccgcg caataaatcg ggaaaagccg
     5401 cggcgtccgc gacctgccgc atgccgcggc cgccgccgcc gccgaccgcc ttgatcatga
     5461 cgggaaagcc gatctttctc gcctcggtca gcatgccgcc atcgctctgg tccgcaccct
     5521 ggtatccggg cacacagggc accccggcgg cctgcatgat cgccttcgcg ccggccttgt
     5581 tgcccatcgc cttgatggcc tcaggcgatg gcccgatgaa caccagacca gcgtcgcgac
     5641 aggcttgcgc gaaatcctca ttttcggcga gaaagccata gccgggatga accgcatcgg
     5701 ctccgccagc cttcgcggcg gcaatgatcg cctcgatccg caaataggat tgcgccggta
     5761 gcgccccgcc gatgcggatg gcctggtcgg cggcccgcac atgcagcgcc tcgcggtccg
     5821 catccgaata gaccgcgacg acgccaaagc ccaaccggcg cgcggtacgc atgatgcgca
     5881 gcgcgatctc gccgcgattg gcgatcagca tggtgcggaa cggcgtgcgc ttcatcggcc
     5941 catcgctcat ggccgcgcaa ccgaaaactg catgcgctgg gggttgcgcg cctcggcctc
     6001 acggcagatc gccagcactt ccgccagaat gccgcgggta tcgcgcggat cgaccacgcc
     6061 gtcgtcaagc acgcgcgcgc tggtcgaaaa tacatccatc tggccatcga acacgccgat
     6121 aatctgcgcc ttcatcgcat cgagtttttc cttctcgacc ggcttgccgc gccggatggc
     6181 ggcggcttcc attacaatcg ccatggtctc ggcggcctgc tcgccgccca tcacggcggt
     6241 cttggcgttg ggccatgaga aacagaagcg cggatgaaac ccgcgcccgc acatgccgta
     6301 attgccggcg ccgaacgacg cgccgcagta gatcgtgatc tgcggcacgc cggcgctggt
     6361 caccgcctgg atcatcttcg agccgtgctt gatcatgccg gcttcctcat aggctttgcc
     6421 gaccatgtag ccggtggtgt tgttgagata gagcagcggc gtccgcgact ggcagcaggc
     6481 ctgaatgaaa tgcgtcgcct tgttggcgcc ggcgggatcg agcggaccgt tattggtgac
     6541 gatgccgatc gcctctccct cgatgcgcgc gtgcccgcac accgtggcgg gaccgtaatt
     6601 ggcgccgaac tcggtgaaat cggagtcgtc gatgattcgc gcgatcacct gccgcatgtc
     6661 gaccggccgc ttgtgatcca tcggcatgat ccccagcagt tcctcgggat catgctgcgg
     6721 cggacgatgc ggcgttttag ctgggctcgg ccggtcccat ctgagattgg ccatgatgtc
     6781 gcgcgcgatg cggagcgcat cgcgatcgtc ctcggcgaga tagtccccga gtccggaaat
     6841 gccggtgtgc atctcggcgc cgccgagttc ctcttcggtg gcgatttctc cggttgcggc
     6901 cttgagcagc ggcggtcctg cgagaaacgc gcgggtgcgg ccgcgtacca tcacgatgta
     6961 atccgacaac ccggtctgat aggcgccgcc cgcggtcgac gaaccatgcg tcaccgtcac
     7021 caccggcaag cctgccgccg acagccttgc cagattgcga aagatattgc cgcctcgcac
     7081 aaaatcctcg acgcggtagc gcagcaggtt ggcgccggcg ctttcgacca gttgcacgta
     7141 aggcagcttg ttctcaagcg ccagttcctg cacccgcagc gtcttgtcga ggccgtaagg
     7201 ctgcagcgca ccggcatcga tgccggaatc attggcgctg accatgcagc ggacaccgga
     7261 aacgaatccg atgccggcga tcaccccgcc gccaggcacg ctcttgtcag cgtcgggaac
     7321 atcgaacatg tagcccgcaa gcgtggagag ttcgatgaac ggcgagccag gatcgagcac
     7381 cagcgtcacg cgttcgcgcg gcagcaattg tccgcgcttg tggaagcgat ccttggcggc
     7441 ggccgaggcg gcgcgcgtcc gttcttccag gccacgcatg cgcgcgatca gcgccagcat
     7501 gccggcgcgg ttggctttga aggcatcgct gcttggggcg atggtcgatt caaggatggc
     7561 cataaggatc aatcaccctc gcgcaaacga caacaatcgt cattccccga tgtgcaattg
     7621 cacatcgtgg atgcgcgtag cgcaggtccc gaatgacgtg tggctggctt gacccaaaca
     7681 aattcaacgc ctgattccaa atatctgtct tgcttccgcc gggctcgcga tctcgcggcc
     7741 ggcgcggcgc gcacatgcgg cgatgctctc gatcaactgg ccgttcgatg tcaccttggc
     7801 gccatcgggc tgatagaagg tatcttcaag cccggtgcgc aggtgaccgc cgaactcggc
     7861 acagcgctga tgcagcggcc agatttcggc gcgcccgatc gcggtgactt gaaaatgcgc
     7921 ttccggcaat ttcagcttca acaggatcgg cagcaattcc ggatcggccg gcatgcctga
     7981 ggcgacgccc atgacgaaat tgtattccag cggtccggag tacatgccgg tctggcggta
     8041 catcccgacg cagcgcacga tgccgacgtc gaagcattcg aattccggaa tcgtgtccac
     8101 ttgcttcatg acgtcgagat aggtctgtac cttctcgacg gcgttatcga acatcatcgg
     8161 cggccacgcc caggtgttgt cgctcttcac tttaagataa ttcagcgagc cggcgttgca
     8221 ggcggccatc tccggtctgg tctcgcgcac gcaatcgagc gcgccggaat aattcgggcc
     8281 tgaagtgcct gtggtgtgat tgatgatgac gcccggacag ccctcgcgga tcgcctgctg
     8341 gatttcgcgg gacacgctga cttcccatga cggcagatgt cccttgtccg gctcctgctg
     8401 gcgcagatgg atatgcatca cgctggcgcc ggcgttgaac gccgccttgg cctcgcgcgc
     8461 catttcctcc ggtgttaccg ggacgtggtg ctgtttcggg tcggtcagca cgccgttcag
     8521 cgcgcaggtg atgacagcct tgtcgctcat ggatcggcct tttgtcgctc gaggtcttca
     8581 cccgcaggct ggcggatgat gcgtccagca ccgcctggct tcttgcgtcc acgcgctatc
     8641 gcggaccagc gccgccgcta tggccggccc tgacggaaac cggcactgtc gcgcatgccg
     8701 ggatcgctgc ggtagatcgc cagatccctt gagccggaaa agatcgcgcg cggctgcaag
     8761 ccgtagaaac ggcggatcga gtggctgaaa tgcgtcgagt cgggatagcc gatgtcctgc
     8821 gcgagatgcg ccagattgat gtcttcgttg acgaagtgca gcagatgccg cgcgcgcttc
     8881 caggcgcgaa atgcccgaaa cgagatgccg gtttggtcct tgaacagatg caggaagcgc
     8941 gatggcgaca ggtggaccga tgcggcgcag tcggccgcgg tcatcttgct gccggagaaa
     9001 tcattgagca tcaaagccgc gcttcggatg cgatgatcca tgctgcgatc gggcaaacgc
     9061 tcgccaaacg acagctggtc gaactcttga gtggtaaaac cgccacgccg ctcttgccgg
     9121 cgcaaggtct cgtaagcggt acgaatgcgc tgcgcgatgt cgggcgcttc cgctccgcga
     9181 attcttgcgg ccaagccatt catggccgag ggctcgaccg tttccggctc gatcacgagg
     9241 caaatgatcg agggatgctc gctttcaacg ctgtggggca cgtaaggccg aaccagcgcc
     9301 acttcaccga ccacctcgga cttgcccccg gtgatctcga gacttccctg catcacagtg
     9361 tagatgttga agccaccact ggatcgtttt cgcggccggc ccaacaggcc cgcataaaac
     9421 acccgctcgg ggctgatcag catcagccgc cccgaatcac ggcgatccct gtcgttcatg
     9481 gtccatccct cccgggagcg atttctggtt cttgccgcgt tcgcgcgggg ctctctcagt
     9541 ggagctgcgg gcagaataca gaattcgcgc ctgatgtcac cgatgatcga cgccttcggg
     9601 ccaatgccgc gatcgaacct cttcctgtca cggctcaaac gggccggata tccggatatc
     9661 atggcaagac cccttcaggg gacggtgcgc gcagcatgaa tagcgccgcg atagtaagca
     9721 gcgccagaag caagccgacc gcgcagacgc ccggccactg gtataagctc caggcataat
     9781 tgcccgccgc tgagccgagc gccccgccaa caaagcgggt gaccatgtac accgtattga
     9841 gccggctcat ggcttccgcc ttgaggctat agttgcgggt catgttggag acgtggcccg
     9901 actgcatgcc aaggtcgagc aacaccacgc cggcggcgac acccgcaagc gagtaacccg
     9961 ccgttgcgag caccatgaac gccgcgagca caaccaggct gctcagcaat acggtcaggc
    10021 gcggacttcc gaggtcggcc aatttgccgg ccagcggcgc cgcggccacg cctgcaatcc
    10081 cgacaagtcc aaacagtccc gcaacatcgc tgccgtaatg ttccggcatg ctgttgagat
    10141 gaaacgccag ggtggtccag aacgcactga acgccgcaaa ctgcagcgcc gccgtcagcg
    10201 aggtctcgcg aagaaccggc tgttccctga ccagtcccca gagcgatttc atcaggccgg
    10261 aatagttgcc atcgaacact ggcttgtcct ttggcaacag caccgacaac accggtatca
    10321 gcaagaccat cgtgccgccg gcgatccaat acatcgcagg ccagccgagc agcgttgcga
    10381 tggcacctgc gatgctgcgt gacagcagaa ttccggccaa cagtccgccc atcacgatcc
    10441 cgacgacgcg cccgcgttcc gccggcagcg ccagatgggc ggcaaaggga acgatgacct
    10501 ggggtaccgc gctggtgaag ccgacggcga agctcgcaac cagcagccac tgcatcgtcg
    10561 gcgcccgcgc ggcggcgacg agggcgccgg ccgagcccag cagcaaggcg ttgatcaaca
    10621 gccgccggtc gatcatgtcg ccgagtggca gaaacaccag catgcccagc gcataaccgc
    10681 cctgggtcat gatggaggcg ccgcccgcct ctcgctcggt tacatgaaat gttctggcga
    10741 aatctgccag caagggctga ttatagtagt tattcgcaat aacaatcgca cacgccgcgg
    10801 ccaacagcca ggtgcccgcc ctacgcatcc caactgcccg cgaccacgac aaccgcgcag
    10861 gccgccgcgg ctatgatccc ggccgctgga aacgcgattg caaattcatg cggggaggtc
    10921 tcagccattc gaggcaatcc gagcacgaac aggcttgccg ccaggccggc caatgaccat
    10981 ccgcacacga ccatgagcga agcagattca gggtccccat gttgatgtgt ccgctcggcc
    11041 atggtttcgc tccgaaaggc tgttcgtgca cggatgggta ggacagttgc gcgagccggg
    11101 taagccattt aggttgcaca ggatccttgc aggtctcgca agaatgggag ccgatggacc
    11161 ggttggatgg aatggcggcg ttcgccgccg ctgtcgaagc tggcagtctg tccggcgcag
    11221 ccaagcgcct gggatgttcg ctggcgtcgg tcagccgtca gatcagcgcg atcgagcggc
    11281 atttcggcgt caccttgctg gccaggacca cgagacgcct cgcgcttacc gacgaaggca
    11341 gggactacta cgagcacgtc aagcgtatcg tcagcgaggt cgtcgacgcc gagcgcacgt
    11401 tgtcggcaca ggcgaaggtg cctcacgggc ggctgcgcat cagcgcacca acattgatcg
    11461 gccggctgcg gcttgctccc ttgttgtccg cgtatctggc cgatcaccct caggtctcga
    11521 tcgatctgac gttgctggac cgactgccca acctcttgga ggacgagatc gaccttgcca
    11581 tcgttctcgg ccatctgccc gattcgtcgc tggttcgtcg cagcctcggt catgttcgcc
    11641 gggttttgtg cgcggcgccg gactacctcg ctatccgcgg catgcctgcc acgccgcagg
    11701 atctcgcgac ccatgattgc ctgatcttca cgacaacgcc gggatcggcg acatggacgt
    11761 ttcagatttc gggacgacga accgaaatcg aagtaccggc gcggttgcgt tccaatgcgc
    11821 tcgatgtggt ggtctccgct gcgctgggcg gcagcggcat tgcgcgcgcg ccatcctggc
    11881 aggtgcagga tcatgtcgat gccggcaggc tgcaattgat gttgcaggat tacgagcggc
    11941 catccttgcc cttgcagatg atcttctcgg acccccgcca tttctcacga aaagttcagt
    12001 cttttgcgga ctttctcgcg cagcaatgga atggcgacaa gcgctgacgc tgtcgaaacc
    12061 acgcgggcgg ctcacgagcg ccgcgcgaca ttttgctgct cgccggcgag cgtttccgcg
    12121 gcgacggccc gcttgtatcc gtcgcgcgcc tgcaggcgct gccagtaggc ggcgacgttg
    12181 ggcccgaaat cctttgccag ggcgatgttg tcggcaagcc gcaacgcgta gccgatcacg
    12241 atgtcggccg cggtgaagcg atcggcgcac agcgtctctg ccttggcggt ggcggcctcc
    12301 acggcgcgca gccgccccaa gaaccatttg gcgtaatcgg tggcgacttg cggattgcgc
    12361 cgctcctcgg gttcgagctg ggaatagcgc agcaccagcg tctgcggaaa ggtcagcgtc
    12421 gcatcgctga aatacatcca gttcaggaag gcgccatagg ccggctcgtc gaggcccacc
    12481 atcagcggcg tcggtccata cttcgtgccg agatagtgac agatgccgga agactccgtc
    12541 atcttggttt cgccatcgat catcaggggt atcgtcccga gcggattgag cgcgagatat
    12601 tccttggcga acacccgcgg cgggaacggc agcattttga gctcataagc gagccccagc
    12661 tcttccagca tccacagcgg ccggaacgag cgcgccgcgt ggcagtgata aagtgtgatc
    12721 atcggcgttt ccctgctatc tatcattcac ttttgattga ggcttggcaa tgtgcccatc
    12781 atcttgcaca gcaccgtcag catgatttcg tcggcgccgc cgccgatcga ggtcaggcgg
    12841 ctatcgcgat aggcgcggct caccagcgtc tcgttcataa aacccatgcc gccccagaac
    12901 tgcaggcagg cgtcggtcaa ttcgcggccc aaccggcccg ccttgagctt ggccatggtc
    12961 gccaatctcg tcacgtcctc gccggcgacc agcgcttctc cggcgcgata gatcagcgcg
    13021 cgcaacagct cgacctcggt ctgcatttcc gcaagcttga agtgaaccgt ctggttgtcg
    13081 aggatgctgt ggccgaacgc gttgcggccc cgcgtgtatt cgatcgtctc gtcgatgatg
    13141 gtttcatgcg ccttgaggca ggcggccgcg ccccacagcc gctcctcctg gaattgcacc
    13201 atctggtagg taaagccgtg gccttcctcg ccaatccgat tccgcttcgg cacccgcacg
    13261 tcgtcgaaga aaatctgcgc ggtgtcggac gagcgcatgc ccatcttgtc gagttttcgc
    13321 gcgacttgca cgcccttggt tttcatcggc acgcagatca gcgacttgtt gcgatgtttg
    13381 ggcgcgtcgc cagtattggc caacagacag atccagtcgg cctgcacccc gttggtgatc
    13441 cacatcttgc cgccattgat gacgtaatcg
//
LOCUS       DATNOI010000038         5184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_704991,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000038 DATNOI010000000
VERSION     DATNOI010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704991"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VN968_01420"
     CDS             <1..431
                     /locus_tag="VN968_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HXL27941.1"
                     /translation="TYRGVLNAQRAEPEPTVGDAIADAARQIAETLDLSAIICWTSSG
                     STALRVARERPKPPVVAITPNLATGRKLSVVWGVHCVVAEDAKDLDDMVNRAGSIAFR
                     DGFARAGQRVIIVAGVPLRAPGTTNMLRIASVGPSDDANM"
     gene            complement(561..989)
                     /locus_tag="VN968_01425"
     CDS             complement(561..989)
                     /locus_tag="VN968_01425"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXL27942.1"
                     /translation="MTTTFGSAKWQGGIKDGKGAISTKSGALKDYPYGFASRFEGKPG
                     SNPEELIGAAHAACFTMALSLILGEAKLTAEQMETKADVTLEKVGDGFAITSVHLALT
                     AKIPGADKAKFDELAGMAKAGCPVSKLLNTRITLDATLLS"
     gene            1071..1532
                     /locus_tag="VN968_01430"
     CDS             1071..1532
                     /locus_tag="VN968_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27943.1"
                     /translation="MRPWLASIATIVIWMLVGATPATAGYWNYGCKGSLGDTALTFDR
                     NTFLIMPKALATGDIAGLARSDIFAFDADDNNSGFMTTMKFARGADPDQKIVLTEKSS
                     KKISEQEGHVGSREKTTTTFSKTYHYERLGWTGKPEADIVMACIEYQLTAP"
     gene            complement(1536..2210)
                     /locus_tag="VN968_01435"
     CDS             complement(1536..2210)
                     /locus_tag="VN968_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL27944.1"
                     /translation="MVYRRTQQVVKRLAARRSAILAAARDAAADGGMAAVQIAPVAIR
                     ANVAAGTVYRYFPSKADLISELIADVSRDELAAIRRAADAAPGPSSALAAAVTTVAVH
                     VLSQRKLAWGILAEPVDVDVTASRLQSRREIAGEIAARIEAAVRAGHLPAQDTGLAAT
                     ALLGALHESLVGPLAPDHSDDPARLRDAVQTVTLLALRAVGVMDARARGLVVQAVLPA
                     KTLVGA"
     gene            complement(2361..3020)
                     /locus_tag="VN968_01440"
     CDS             complement(2361..3020)
                     /locus_tag="VN968_01440"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXL27945.1"
                     /translation="MNGTRIALVGLVAACAWAAWPKREIVIDTQRISRQLVVWRTLAR
                     VDDPIVILGDSIVEASTLPRSLCGHPIVNAGIGGATTASNLGSFLADALGDKRAALVV
                     VSLGGNDAAVSDSVERYRSAYRALLTELKALTPRRAMVGISPVEAGLEDGKKISGAVI
                     DSFNAILPELAKEAGAAFIPMPVMPEHHTLDGIHLDAAGYAIWDKAVLGGVESAVCKS
                     S"
     gene            complement(3017..4303)
                     /locus_tag="VN968_01445"
     CDS             complement(3017..4303)
                     /locus_tag="VN968_01445"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXL27946.1"
                     /translation="MSGPSRQYPESRGDKVPRIAYLESIRGLAALQVLLLHFLGAFAP
                     DLVVSLPAGAGIAGYIHLSPLYFLYDGYSAVYIFFALSGYVLTRAFERHLARPSSQIL
                     ARIVRLGLPAIAATLVAAVVMLTFGKPNVPAGELSGSVWFASQWNSDLSVLSVIRDGT
                     INALFLGYSGLPGVAFLAPWQQPVENSLVAPLWTLSIEFYGSMIVLGLCWCARRSRAL
                     WWLAMLLGVIFTIRSAYICFFVGHLLATVQRAETPAPVSRLLPMLAVMFGVPLCVLAE
                     VWQPQWLRSLCADPTYFLFPGQFAPMQLKTYGALLVLVGVIDLQAARRFLSRPWLVAR
                     SKLSFPLYLIHWPILFGPSAALFLLLNSLVGIELARLCAIVAGICLAFAGSLVFLGVD
                     RRALELSRTLRRRMSDAVHETPRERTSRADRIVPAE"
     gene            complement(4300..>5184)
                     /locus_tag="VN968_01450"
     CDS             complement(4300..>5184)
                     /locus_tag="VN968_01450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HXL27947.1"
                     /translation="AFIGWMAASLVPYLAVIRGIVGRPFGLMLAIAFPMVFSNTLVGQ
                     NGFLTAALIGGTLYLIPARPILAGVCLGLLSYKPQYGLLFPLVLIAASQWTVFFTAGA
                     VAVAMALASWLAFGTESWQAFFHWMPMFSQAFLTEGRAPWGKMQSIFAVVRYFGGTEP
                     LGWLFQWIMSATVAVVLTLVWRSRISYPLRAAALATGTLLITPYLFLYDLMVLAIAMA
                     FLVRIGLSNGFERHELPALGLAVALLMFYPLVGAPTGFVATLIVSGLIAARCGFWRSR
                     RVSSPGVGLATALPTPDR"
BASE COUNT         1070 a         1677 c         1625 g          812 t
ORIGIN      
        1 cgacctatcg cggcgtgctc aacgcgcagc gcgcggagcc ggaaccgacc gtcggcgacg
       61 ccattgccga cgcggcgcgg caaatcgccg aaacgctcga tctgtccgcg atcatttgct
      121 ggaccagttc tggatcgacc gcgctgcgcg tcgcgcggga gcggccgaag ccgccggtgg
      181 tggcgatcac gccgaacctc gcaaccgggc gcaagctgtc cgtggtctgg ggcgtgcatt
      241 gcgtcgtcgc ggaagacgcg aaagacctgg acgacatggt caaccgcgcc ggctccatcg
      301 cgtttcgcga tggttttgcc agggcgggcc agcgtgtcat catcgtcgcg ggcgtaccgc
      361 tccgcgctcc cggtaccacc aacatgctgc gcatcgcctc tgtcggcccg agcgacgacg
      421 ctaatatgtg aagcgggcag ccgctgagtt ttccaccgtc attccggtgc gcgtccaacg
      481 ggtccgcgca aagcgcggcg acgcgaactc ggaatctcga gattccgggc tcgcgctaac
      541 gcgcgccccg gaatgacaac tcagctcagc aacgtcgcgt ccagcgtgat cctggtattc
      601 aacagcttcg ataccggaca tccggctttg gccatgcccg ccagttcgtc gaatttcgcc
      661 ttgtcggcgc ccggtatctt cgccgtcaaa gcgagatgca ccgaggtaat ggcaaaaccg
      721 tcgcccactt tttcaagcgt gacgtcagcc ttggtctcca tctgctcggc ggtaagtttt
      781 gcttcgccta atatcagcga cagcgccatg gtgaaacagg cggcatgcgc cgctccgatc
      841 agttcttccg ggttcgagcc cggcttgccc tcgaagcggc ttgcgaaacc gtaagggtaa
      901 tccttcaacg cgccgctctt ggtcgagatc gcgcccttgc cgtccttgat accgccctgc
      961 catttggccg atccgaatgt cgtggtcatg cgctgctcca tcggtgagtt gaacaagtag
     1021 cggctcttaa gcgaccaata cggcggaaga tgtcaattca cgagggcgac atgagaccct
     1081 ggcttgcctc catcgcaacg attgtaatct ggatgttggt cggcgcgacg ccggcgacgg
     1141 cgggttactg gaactacggc tgcaagggca gcctcggcga cactgccctc accttcgacc
     1201 gcaacacgtt tctgatcatg cccaaggcgc ttgccacggg agacatcgcg ggattggcca
     1261 gaagcgatat cttcgccttc gacgccgacg acaacaattc cggtttcatg accacgatga
     1321 aattcgcgcg cggcgccgat ccggatcaga agatcgtcct gaccgaaaaa tcatcgaaaa
     1381 aaatctccga acaagaaggc catgtcggta gccgcgagaa gacgacgacg accttttcga
     1441 aaacatatca ttacgagcgg ctcggctgga ccggcaaacc cgaggcggac attgtcatgg
     1501 catgcatcga ataccagctg acggcgccgt aacccctacg cccccaccag cgttttcgcg
     1561 ggcagtaccg cctgcaccac gaggccgcgg gcgcgggcgt ccatcacgcc gacggcgcgc
     1621 aatgcgagca gcgtcacggt ttgaacggcg tcgcgcaatc gtgcgggatc gtcgctatgg
     1681 tccggcgcca gcggcccgac cagcgattca tgcaacgcgc caagcagcgc ggtcgcggcc
     1741 aagccggtgt cctgcgcggg cagatgtccg gcgcggacgg cggcctcgat cctggctgcg
     1801 atctcgccgg cgatctcgcg gcggctctga agccgcgacg cggtgacatc gacatcgacc
     1861 ggctcggcca ggatgcccca tgcgagcttg cgctgcgaca gcacgtgcac ggcgacggtg
     1921 gtaacggctg cagccagcgc cgaggatggc ccgggggcag cgtccgccgc gcggcggatc
     1981 gcggccagtt catcgcgcga gacatcggcg atcagctcgg aaatcagatc cgccttggag
     2041 gggaaatagc ggtacacggt gccggcggcg acattggcgc ggatcgcgac cggcgcgatt
     2101 tgcactgcgg ccatgccgcc gtcagcggcg gcatcccgcg ccgccgccag aatggcgctg
     2161 cgacgcgccg caagccgctt caccacctgc tgggtccgcc ggtaaaccat ggcgtgtttc
     2221 tcgtccctgg gcgcattccg atgccggttg ggagccggtc gagaacgcgt gtcttcggtg
     2281 ttttgggcga atttttcgca attcgcttct aagaactgaa cacctattca ggccggctga
     2341 caagatggtt cacgaccgtt tcaggaggat ttgcagacag cggactcgac gccgcccaag
     2401 acggccttat cccagattgc atagccggcg gcatcgagat ggatgccatc gagggtatgg
     2461 tgctccggca tgaccggcat aggaatgaac gcggcgccgg cctctttggc gagctcgggc
     2521 agaattgcgt taaagctgtc gatcaccgcg ccgctgatct tcttgccgtc ctccagtccg
     2581 gcttcaacgg gcgatatccc caccatggcg cggcgcggcg tcaacgcttt gagctcggtc
     2641 agcagcgcgc ggtaggctga ccggtatcgc tcgacgctgt ccgacaccgc cgcatcgttg
     2701 cctccaagcg agacgacgac cagtgcggcc cgcttgtcgc ccagcgcgtc ggcgagaaac
     2761 gagccgaggt tgctcgcggt cgttgcgccg ccgatcccgg cgttgacgat cggatgaccg
     2821 cacagcgagc gcggcaaggt cgatgcctcg acgatgctgt cgccgagaat gacgatcggg
     2881 tcatcaaccc gcgccagcgt acgccagaca acaagttgtc gagatatccg ctgggtgtcg
     2941 atgacgattt cgcgcttcgg ccatgccgcc caggcgcagg ctgccaccaa cccaaccagt
     3001 gcgatgcgcg tcccgttcat tcggccggca caatgcgatc ggcacgggac gttctctccc
     3061 gcggcgtttc atggaccgca tcggacatgc gccgtcgcag tgttctcgac agttcaagcg
     3121 cccggcgatc caccccgaga aaaaccaggc tgccggcaaa ggcaaggcag atcccggcca
     3181 cgatggcgca caggcgcgcc agttcaattc cgaccaggct gttcaacagc aggaataaag
     3241 cggctgacgg cccgaacagg atcggccagt ggatcaaata cagcggaaac gacagttttg
     3301 accgggccac cagccacggt ctggacagaa aacgccgtgc cgcctgcaga tcgataaccc
     3361 cgaccagcac gagaagcgcg ccataggtct tcaactgcat cggcgcgaac tggccgggaa
     3421 acaggaaata ggtcggatcg gcgcacagac tgcgcaacca ttgcggctgc cacacctcgg
     3481 cgagcacgca cagcggaaca ccgaacatga cggcgagcat cggcagcagc ctgctgaccg
     3541 gcgcgggcgt ttcggcgcgc tgaaccgttg ccaggagatg accgacgaaa aagcagatat
     3601 aggcgctccg gatcgtaaag atcacgccga gcagcatggc caaccaccac agcgcgcgcg
     3661 atcgccgcgc gcaccagcac aggcccagaa cgatcatcga gccgtagaac tcaatgctga
     3721 gcgtccacag cggggccacc aatgaatttt cgaccggctg ctgccacggc gccagaaaag
     3781 cgacgccggg caatccgctg tagccgagga acaaggcatt gatggtgccg tccctgatca
     3841 ccgagagaac agacaggtcg gaattccact gactggcaaa ccagaccgac ccgctcaact
     3901 cgcccgcggg cacgttcggc ttgccgaacg tcagcatgac gaccgccgca accaacgttg
     3961 cagcgatcgc gggcaggcca aggcgcacga tgcgcgccag gatttgcgaa gatggccgcg
     4021 ccagatgacg ctcgaaggcg cgggtgagga catagccgct gagtgcaaag aaaatgtaga
     4081 ccgccgaata gccgtcatac agaaaataca gcggcgacag gtgaatatag cccgcgatgc
     4141 cagcgcctgc cggcagcgat accacaaggt ccggcgcgaa ggcccccaga aaatgcagca
     4201 gcaggacttg cagggctgcg aggccccgga tcgattcgag ataggcgata cgcggtacct
     4261 tgtcgccacg gctttcggga tactggcgcg aaggaccgct catcggtcgg gcgttggcaa
     4321 cgccgtcgcg aggccgacac cgggcgatga cacccgcctg ctgcgccaaa acccgcagcg
     4381 cgccgcgatc aatcccgaca cgatcagtgt tgccacgaag ccggtcggcg cccccaccag
     4441 cggatagaac atcagaagtg cgacggcgag gccaagtgcc ggcaattcat gacgctcaaa
     4501 gccgttgctc agtccgatcc ggaccaggaa cgccatcgca atcgccagca ccatcaggtc
     4561 gtagagaaac aggtatggcg tgatcagcaa tgtgccggtc gcgagcgcgg ctgccctcaa
     4621 cgggtagctg atacggctgc gccacaccag cgtcagcacg accgcgacgg tggcgctcat
     4681 gatccattgg aacagccagc cgagcggctc ggtgccgccg aaataccgca ccaccgcgaa
     4741 aatgctttgc atcttgcccc agggcgcgcg gccctcggtg aggaaggctt gcgagaacat
     4801 cggcatccag tgaaagaacg cctgccagct ctcggtgccg aaggcgagcc atgatgcaag
     4861 cgccatcgcg acggccacag cgcctgcggt gaagaacacg gtccattgcg aggccgcgat
     4921 cagcaccagg ggaaacagca gtccgtattg cggcttgtag ctgagaaggc cgaggcacac
     4981 gccggcgaga atcggccgcg ccgggatcaa atacagcgtg ccgccgatca gtgccgcggt
     5041 gagaaagccg ttctgcccga ccagcgtatt gctgaacacc atggggaaag cgattgcgag
     5101 catcaggcca aacggccggc cgacgatgcc gcggatgacg gcgagataag gaacgaggct
     5161 tgccgccatc cagccgatga aggc
//
LOCUS       DATNOI010000039         3637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_702514,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000039 DATNOI010000000
VERSION     DATNOI010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702514"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VN968_01455"
     CDS             <1..1023
                     /locus_tag="VN968_01455"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HXL27948.1"
                     /translation="IDFDLSGCGLDYVVGDSFGIFARNELGLVDQIIAMLGASHTAEV
                     KGKTLREVLTDDVSLAPAPDSLFELLSFITGGAQREKARALAQGEDPDGDAATLDVMA
                     ALQKFSGVRPHPEAFVEALEPLQPRLYSISSSHNATPDKLSLTVDCVRYVVGKRRRLG
                     LASTFLAERIKPGDPVKVYVQKAHGFALPQDPGTPIIMIGPGTGIAPFRAFLLDRKAT
                     GASGKNWLFFGHQRSACDFLYSDELNAMKTSGQLTRLSLAWSRDGDKKFYVQDRMREV
                     GRELWTWLAEGAHLYICGDAKRMAKDVERALVDIVAQFGARTTDEAVGFVGELKKRGR
                     FQQDVY"
     gene            1201..1353
                     /locus_tag="VN968_01460"
     CDS             1201..1353
                     /locus_tag="VN968_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27949.1"
                     /translation="MRELSAEARLHLYARSLSRRTGTGFHAAALYGAFALIAAVIFGT
                     LSVHPF"
     gene            complement(1406..2689)
                     /locus_tag="VN968_01465"
     CDS             complement(1406..2689)
                     /locus_tag="VN968_01465"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXL27950.1"
                     /translation="MLLATVAIVFAALVLLSMPIVFALGVAGIAGLWIGGYPMQQFSS
                     ALVSGSQSWVLLAIPAFVFAGNLMERCGMSHALVELARALIGWVKGGLGMSVIVVAYF
                     FSDICGSKMAEVSALGSALMPPLTRAGYDRRDSASLIAAGTAMGMLVPPAIFMIVIAQ
                     VTNTSAVALFVAGFIPAVVIMLCLMAVVYVRARQNDWPVDSRPSLKRLGHAAVHAAVP
                     MVVPFVILAGFILGIITATEAGAVVAGYAFLAARLYYRNVSWREMGKIAYDSAILTAA
                     VVFLLAVASVYQYLMGVSGVPQLLGQVLGPLKTHPWLFLIGTAVMSCLFGMLLEGLPA
                     AVVLIPVVFPIAEAMGIDPIHFNIVQTAAVGIGLFLPPMGVGLLMALRFAKLSIGQHF
                     RTYVPYMLALFAGLLLIICLPEISLTLPRLAGLIK"
     gene            complement(2702..3199)
                     /locus_tag="VN968_01470"
     CDS             complement(2702..3199)
                     /locus_tag="VN968_01470"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXL27951.1"
                     /translation="MLRILSAVPKLTVTALIILAIVNLLVGVILRYFVGAITGWLDVD
                     PVPFTWVEEVGELSLAWLTLIGAAIGIQSRTHFTLTVFVHRLPEIAQLWIHRFNHALI
                     TGVGLLAAWYGFKLCLLNRTLTTPGLEINLAWLYASSVVGGILIAVYGFAMMISPLPG
                     GEPQH"
     gene            complement(3284..>3637)
                     /locus_tag="VN968_01475"
     CDS             complement(3284..>3637)
                     /locus_tag="VN968_01475"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27952.1"
                     /translation="IVNMKALKIYEVSKYASLTYHNYGPTNAVMNLDIWNGLSAEHQK
                     LIMDLSRAAQEKIREATESVDNFAKAKSELEPLGMIVVEAKVDEFRKVAQQKIWPAYK
                     AQYGALWDEIEGFKV"
BASE COUNT          744 a         1130 c         1131 g          632 t
ORIGIN      
        1 atcgatttcg accttagcgg ctgcggcctc gattatgtcg tcggcgattc cttcggcatt
       61 tttgcccgga acgaactcgg gctcgtggac cagatcatcg ccatgctcgg cgcatcgcac
      121 accgccgaag tgaaaggcaa gacgctccgc gaggtcttga ccgatgatgt ctcgctggcg
      181 cctgcaccag attccctgtt cgaactgctc tcgttcatca caggcggcgc gcagcgcgag
      241 aaagcgcgcg ccttggcgca aggcgaggac cccgacggcg acgccgcgac actcgacgtg
      301 atggcggcct tgcaaaaatt ctcaggcgtt cgtccgcatc ccgaagcctt cgtcgaagcg
      361 ctggagccac tgcagccacg gctctattcg atctcctcgt cgcacaatgc gacgcctgac
      421 aagctgtcgc tgacggtgga ttgcgtgcgc tatgtggtcg gcaaacgcag gcgcctgggc
      481 ctggcttcga ccttccttgc cgagcgcatc aagccgggcg acccggtgaa ggtctatgtg
      541 cagaaggccc atggcttcgc gctgccgcag gatcccggaa cgccgatcat catgatcggg
      601 ccgggcaccg ggatcgcgcc gttccgcgcg tttctgctcg atcgcaaggc gaccggcgca
      661 tccggcaaga actggctgtt cttcggtcat cagcgcagcg cgtgcgattt cctctattcc
      721 gacgaactca acgccatgaa aacctcgggc cagctgacgc gattgtcgct ggcgtggtcg
      781 cgcgacggcg acaagaaatt ctatgtgcag gatcgcatgc gcgaggtcgg ccgcgaattg
      841 tggacctggc tcgccgaagg cgcgcacctc tatatctgcg gcgatgccaa gcgaatggcc
      901 aaggatgtcg agcgcgcgct ggtcgatatc gtcgcgcagt ttggggcgcg cacgaccgat
      961 gaggccgtcg gctttgtcgg ggaactcaaa aagaggggcc ggttccagca ggacgtttat
     1021 tgaggcagtt tcaatcaacg tcaaatctga acgaataaaa ttctcgaccg aattcgcggc
     1081 ggagaatttt cagctgctac cggcctcagg cggagcattt gtatcgctat tttcccggcc
     1141 accttgaatc ccgacgcgaa cagtattctt ttactgccga tggggcgttg gagatcgacc
     1201 atgcgcgaac tatcggcgga agcccgcctg cacctgtatg cgcgctccct gtcccggcga
     1261 accggaacgg gatttcatgc cgccgcgcta tacggagcat ttgcgctgat cgccgccgtc
     1321 atcttcggca cgctgtcagt tcacccgttc tgaccggcgc tacccgcagc tatacgccgt
     1381 tgacattgag cacgacgatc gcgcctcact tgatcaggcc cgccagcctt ggcagcgtca
     1441 ggctgatctc cggaaggcag ataatcagca atagcccggc aaacagggcc agcatgtacg
     1501 gcacataggt tcggaagtgc tgtccgatgc tcagctttgc gaagcgcagc gccattaaca
     1561 gcccgacacc catcggcggc aggaacagac cgatgccgac ggccgcggtc tgcacgatgt
     1621 tgaagtggat cgggtcgatc cccatcgctt cagcgatcgg aaacaccacg gggatcagca
     1681 cgacggcggc gggcaggccc tccagcaaca tgccgaacag gcaactcatg acagcggttc
     1741 cgatcagaaa cagccacggg tgcgttttca gcggcccaag aacctggccg agcaactgcg
     1801 gcaccccgct cacgcccatc aggtattgat agaccgaggc taccgcgagc aggaacacca
     1861 ctgccgcggt aaggatggcg ctgtcgtagg cgatcttgcc catctcgcgc caggagacat
     1921 tgcggtagta gagcctggcg gcgaggaagg cgtagcccgc gaccaccgcg cctgcctcgg
     1981 tcgccgtgat gatgcccagg atgaagccgg cgaggatcac gaacggtacc accattggca
     2041 cggcggcgtg caccgccgcg tggcccaggc gcttgaggct cggcctcgaa tcgaccggcc
     2101 agtcgttctg gcgcgcgcgg acgtaaacca ccgccatcag acacagcatg atgacaacag
     2161 ccggaatgaa gccggcgacg aacagtgcga ccgccgaggt gttggtgacc tgcgcgatca
     2221 cgatcatgaa gatcgccgga ggcaccagca tgcccatcgc agtgccggcg gcgatcagcg
     2281 aggctgaatc ccgccggtcg tagccggccc tggtgagcgg cggcatcagc gcagagccga
     2341 gcgcggagac ctcagccatt ttcgatccgc agatgtcgga gaaaaaatac gctaccacga
     2401 tcaccgacat gccgagcccg ccctttaccc aaccgatcag ggcgcgcgcc agttcgacga
     2461 gcgcgtggct catgccgcaa cgctccatca gattgccggc aaacacaaag gcagggatgg
     2521 cgagcagcac ccaactctgc gagccggaca ccagcgccga ggaaaactgt tgcatcgggt
     2581 agccgccgat ccacagtcct gcgatgccgg ccaccccgag cgcgaacacg atcggcatcg
     2641 agagcaggac gagcgcagcg aagacgatgg cgacagtcgc aagcagcatg aatggccggc
     2701 ctcagtgctg gggttcgccg ccaggcagcg gggagatcat catcgcgaag ccgtagacgg
     2761 cgatcaggat gccgccaacc accgaagacg cgtagagcca ggcgagattg atttcgaggc
     2821 cgggcgtcgt cagcgtacgg ttgagcaggc acagcttgaa gccgtaccac gcagcgagca
     2881 ggccgacgcc tgtgatcagc gcgtgattga aacggtggat ccagagctgc gcgatctccg
     2941 gcaggcgatg cacgaagacc gtgagcgtga aatgcgtgcg tgactggatg ccgatcgcgg
     3001 cgccgatcag ggtcagccac gccagcgaaa gctcgcccac ttcctcgacc caggtgaacg
     3061 gcacaggatc gacatcgagc catccggtga tggcgccgac gaaatagcgc aggatcacgc
     3121 cgaccaagag gttgacgatg gcaaggatga tcagcgccgt gaccgtcaat ttggggacgg
     3181 ctgagagaat acgcagcatg cgtgatggaa tcccgctgat gggacggggc gcggggccga
     3241 agacctggaa gccgaagatt tggcttcggc cccgctgcta actttagacc ttgaagcctt
     3301 cgatctcgtc ccacaatgcg ccgtactggg ccttgtaggc cggccatatc ttctgctgcg
     3361 ccactttgcg gaattcgtcg actttggctt caaccacgat catgccgagc ggctccagct
     3421 cggacttggc tttcgcgaaa ttgtcgaccg actcggtggc ctcgcggatc ttctcctgcg
     3481 cggcgcgtga cagatccatg atcagcttct ggtgctcggc cgacagcccg ttccagatat
     3541 cgaggttcat caccgcgttg gtcgggccgt aattgtgata ggtgagcgag gcatatttgg
     3601 acacttcgta gatcttcagc gccttcatgt tgacgat
//
LOCUS       DATNOI010000040         2098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1928414,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000040 DATNOI010000000
VERSION     DATNOI010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928414"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VN968_01480"
     CDS             <1..569
                     /locus_tag="VN968_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27953.1"
                     /translation="TYQSPLQKLQAELQAEVSSGTISSSDEAALSSALTDIDTALQDS
                     RASDQSSGTRPSPGDLQSKISDLISSEVSSGTLTSDQATELQGVFKAAFANGPGGAGG
                     PPGAGQAGGPPGGPPPGGPPPTDGSSSTDGTDNSSSSTSVDDILKQFLQLLQNSLSAS
                     SSTSYSATGNSTNSTANASSALLIDYQS"
     gene            766..1323
                     /locus_tag="VN968_01485"
     CDS             766..1323
                     /locus_tag="VN968_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HXL27954.1"
                     /translation="MLAKYVTAGLAGSMLFASVALAQNPPAPSDSAGKAPAASESSSS
                     ESSSSFQGDWRASKIAGLSVYNDNNETVGSINDLLMDKSGTIKAAVISVGGFLGVGAR
                     LVAVPFDKIKFANEPVAYTSASNAPNAPNAGSRPASTTTTGSTANPPATATATTKPNP
                     WYPDHAVYNATKDELQKMPEFKYSE"
     gene            1505..1717
                     /locus_tag="VN968_01490"
     CDS             1505..1717
                     /locus_tag="VN968_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HXL27955.1"
                     /translation="MTESVYKVIELIGTSKDSWEKAAAAAVERAGQSLRDLRVAEVVE
                     LDLQLDAKGQIEAYRAKLKVSFKFEN"
     gene            complement(1825..>2098)
                     /locus_tag="VN968_01495"
     CDS             complement(1825..>2098)
                     /locus_tag="VN968_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL27956.1"
                     /translation="HYLKAIEAIGTDEAPKVMAQMRATPINDFFAKNGKIRIDGRMVH
                     DMYLFEVKKPEESKGEWDLYKLIATVPGDEAFRPLDKGGCPLVKTN"
BASE COUNT          413 a          686 c          613 g          386 t
ORIGIN      
        1 acacctacca atcgcctctt cagaagctgc aggctgaact gcaagcggaa gtcagttccg
       61 gcaccatcag ttcgtccgac gaggccgcgc tgtcctcggc gctgaccgac atcgacacgg
      121 cgctgcaaga tagccgcgcc agcgatcagt cgagcggcac ccggccgtcg cccggcgatc
      181 tgcagtcgaa gatcagtgac ctgatctcca gcgaagtctc cagcggcacg ctgaccagcg
      241 atcaggccac cgaactacag ggggtcttca aggcggcctt tgcgaacggc ccgggcggcg
      301 ccggcgggcc ccctggcgcg ggccaggccg gtggtccccc tggtggtccg cctcctggcg
      361 gtcccccgcc cacggacggc tcgtcttcga ccgacggcac cgataattcc tcaagcagca
      421 cttcggtgga cgatatcctg aagcaatttc tccaactgct gcagaactcg ctgtcggcct
      481 cgtcatcgac gtcctacagc gcgaccggca atagcaccaa cagcaccgcc aatgcttcgt
      541 cggcgctatt gattgactac caatcctgac ctctgcgcgg cacgcacccc agaccgccat
      601 cctgcccgaa aggcagggtg gttgtcctaa tccatcgatc atcgcgtgtt cggcggaacc
      661 gggttcttgg ttccgcaatg caacttcgaa aatgcatgct gcaggcggaa cggcggcctg
      721 atctacgggt tttctcctca tcgaacactg atcagggaga tacccatgtt agcaaaatac
      781 gtcacggccg gtctggccgg ctccatgttg tttgcaagcg tagcgctcgc gcaaaatccg
      841 ccggctccct ccgacagcgc cggcaaggcg ccggcggcgt ccgagtcgtc ctcgtcagag
      901 tcgtcctcgt cattccaggg tgattggcgc gcttcgaaga tcgcgggcct cagcgtctac
      961 aacgacaaca atgagaccgt cggctcgatc aacgatctgt tgatggacaa gagcgggacc
     1021 atcaaggccg ccgtcatcag cgtcggcggt ttccttggcg tcggcgcgcg gcttgtcgcc
     1081 gtgcccttcg acaagatcaa gttcgccaac gagccggtcg cctacaccag cgcgtcgaac
     1141 gcaccgaacg caccgaacgc tggcagccgt ccggcctcaa ccacgacgac gggctcgacc
     1201 gccaatcctc cggcgaccgc gacggccacg accaagccga atccgtggta cccggatcac
     1261 gccgtgtaca acgcgaccaa ggatgaactg cagaaaatgc ccgagttcaa atattccgag
     1321 tgaggcgaaa gcttggtcaa aataaacgcg cccggttctg ccgggcgcgg ttttgttttg
     1381 gcggacttgc gcgtttcctt aaggcccttc cttgcccctc tccttgcgcc tttcagcgct
     1441 gatatcccga tgttaggctg atcggcggca ggagcacctg cctaatgccg aacgggagga
     1501 cgacatgact gagagcgtct acaaagttat tgagctgatc ggtacgagca aggactcatg
     1561 ggaaaaggcg gccgcggcag cggtcgaaag ggccgggcaa tctctccggg atctccgcgt
     1621 cgccgaagtc gtcgagctcg acttgcagct ggatgccaag ggccagatcg aagcctatcg
     1681 cgccaaactc aaagtctcgt tcaaatttga gaactgatgc gcgcctgctc tccctctccc
     1741 cgggaacgga gagagggaga cgcatgcaag gtgagcaaag cgaaacgggc ccactacggc
     1801 ttcgctatcg cccgcaaatg acgatcagtt cgtcttcacc agcgggcagc cgcccttgtc
     1861 cagcggacgg aacgcttcgt cgcccggaac ggtggcgatg agcttgtaga gatcccactc
     1921 gcccttggat tcctcgggct tcttgacctc gaacagatac atgtcgtgca ccatgcggcc
     1981 gtcgatgcgg atcttgccgt tcttggcgaa gaagtcgttg atcggggtgg cgcgcatctg
     2041 cgccatcacc ttgggcgcct cgtcggtacc gatcgcctcg atcgccttca gatagtgc
//
LOCUS       DATNOI010000041         2127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_851612,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000041 DATNOI010000000
VERSION     DATNOI010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851612"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VN968_01500"
     CDS             <1..1117
                     /locus_tag="VN968_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXL27957.1"
                     /translation="PSAFCAGADLAELGLAYDMIMAAPAETRDRVAFDHFFRLSEALR
                     GIETAGKPVAAAIAGLALGGGGELALATHHRVMVDDPKVAFGLPESLVGLLPGGGGTQ
                     RLPRLTGIEKALPILLEGARLSGQAAIAAGVVDQLVRAGEEVATAERWVLSHPSASQP
                     WDRPGWRPADVDDAAAIIARKRESVLADTLGHYPAPLAILDCVGEGLPQPFDEAIRTE
                     MQIFSKLIQRREPRNMIRTLFLGRLDYDRLRKAGGIPAPVEQAVAAVSEALQAGGAPD
                     ATLANALARAGFHGPNNAPAFDGVIAQSAFWLEAEPVTDAKRALRERLAKAYAVAGQW
                     RATFSEDQRRAADYLLVTKYGFPAYMAGAFSSAQPGA"
     gene            1180..>2127
                     /locus_tag="VN968_01505"
     CDS             1180..>2127
                     /locus_tag="VN968_01505"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXL27958.1"
                     /translation="MSKPLTGIRVLDLSKVLAGPLCAQYLGDLGADIIKVETVGQGDE
                     TRGWPPFPAPGLGTVFLSANRNKRSIAVDMKTEKGREIVHALAKSADVAIESFGTGVA
                     ERLGIDAPTLRTLNARLIHCSISGFGRSGPLKNSPGYDVILQAFCGVMSMTGDEDGGY
                     IRSPISPIDQMTGVHAFSGILALLYAREKTGKGGEIQVSLFETALGLLGYNLQTFWER
                     GVQPPKCGSSHESLCPYQAFEAADGPIMIGVANDNLWRKFCTVTGLNAIVDDPRFRTN
                     ADRVAHRKETLGHVQAALRQKSVAHWNEVLTDVGVPCAPI"
BASE COUNT          369 a          651 c          713 g          394 t
ORIGIN      
        1 gccatcggcc ttctgtgccg gtgccgatct tgccgaactg ggattggcct atgacatgat
       61 catggctgcg cccgccgaga cgcgcgatcg cgtggcattc gaccatttct ttcgtctcag
      121 tgaggcgctg cgtggaatcg agaccgcggg caaaccggtt gcggccgcga tcgcgggcct
      181 ggcgctcggt ggcggtggcg aactggcgct ggcgacccat caccgggtca tggtcgacga
      241 tcccaaggtt gcgtttggct tgccggagtc tctagtgggg ctgttgccgg gaggcggcgg
      301 aacccaacgg ttgccgcgac tgaccggaat cgagaaggcg ttgccgatct tgctggaagg
      361 tgcgcgactg tccgggcagg ctgcgatcgc tgccggcgtg gtcgatcaac tggttcgcgc
      421 cggggaagaa gtagcgacgg cggaacgatg ggtgctgtcg catccgtcgg cgtcgcaacc
      481 ctgggaccgt ccgggatggc ggccggcgga tgtcgatgat gcagctgcga tcatcgcgcg
      541 gaaacgcgaa agcgttctgg ccgataccct cgggcactat ccggcgccgc ttgcgattct
      601 cgattgtgtc ggcgagggcc tgccgcagcc atttgatgag gcgatccgca ccgagatgca
      661 gatattctca aagctgatcc agcggcgcga gccgcgcaat atgatcagga cgctgtttct
      721 tggccgactc gattacgatc ggcttcgcaa ggccggaggc attcctgcac ccgtcgaaca
      781 ggcggtggcg gcggtatccg aggcgctgca ggccggcgga gcgccggatg caacgcttgc
      841 aaatgcgttg gcccgcgccg gctttcacgg gccgaacaac gcgccggcgt tcgatggggt
      901 tattgctcag tccgcgttct ggctggaggc ggagcctgtc accgatgcca agcgtgcgct
      961 gcgcgagcgg ctggcgaagg catacgccgt ggcaggccag tggcgtgcga ccttctccga
     1021 ggatcagcgc cgcgcggcgg attatctgtt ggtcacgaaa tatggctttc ctgcctacat
     1081 ggcaggcgcc ttctcctccg ctcagccggg tgcctgagtg acgccggctt cgaaacgccc
     1141 gccatcgcgg cgtgcgcaac tccaatcgag agaacagcag tgtcaaaacc cctgaccggc
     1201 atccgcgtcc tcgacctgtc gaaagttttg gcgggtccgt tgtgcgccca atatctcggt
     1261 gatctcggtg ccgacatcat caaggtggag accgtcggcc agggtgacga aacgcggggc
     1321 tggccgccgt ttccggcgcc aggccttggc accgtctttc tcagcgccaa ccgcaacaag
     1381 cgcagcattg ccgtcgacat gaaaaccgaa aaggggcgcg agatcgtcca tgcgctggca
     1441 aaatccgccg acgtcgccat cgaaagtttt ggaacgggtg tcgccgagcg cctgggcatc
     1501 gatgcgccga cgctgcgtac cctgaacgcc cgcctgatcc attgcagcat ctccgggttc
     1561 ggccgctcgg ggccgctgaa gaactcgccg ggctatgacg tgatcctgca ggcgttttgc
     1621 ggagtgatgt cgatgaccgg cgacgaggat ggcggctata tccgcagccc gatctcgccg
     1681 atcgaccaga tgaccggcgt gcacgccttc agcggcattc tggcgctgct gtatgcgcgg
     1741 gaaaaaaccg ggaagggcgg cgagatccag gtctcgctgt tcgaaaccgc gctgggccta
     1801 ttgggttata atctccagac cttctgggag cgcggcgtgc agccgccgaa atgcggctcg
     1861 agccatgaat cgctatgccc ctatcaggct ttcgaggcgg ccgacggccc gatcatgatc
     1921 ggggtcgcca acgacaatct ctggcgtaaa ttctgcacgg tcaccggatt gaacgcgatc
     1981 gtcgacgacc cccgattcag aaccaatgcg gatcgcgttg cgcaccgcaa ggaaaccctc
     2041 ggtcatgtcc aggcggcgtt gaggcaaaag tccgttgcgc actggaacga ggtgttgacc
     2101 gatgtcggcg ttccctgcgc gcccatc
//
LOCUS       DATNOI010000042         4079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_171616,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000042 DATNOI010000000
VERSION     DATNOI010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171616"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VN968_01510"
     CDS             <1..934
                     /locus_tag="VN968_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXL27959.1"
                     /translation="ILMNSPRRPDGPAVYDGKSYSAAVHLAEDVTPFVAIANGLRERG
                     FSAPTIHHADLDTGFLITEDFGSAGLVEGDPSGPTVERYQVATDLLAALHRQVLPFIL
                     PVVPDSTYAIPTFDTDALLIEAGLMLEWYLPDRGAKPADEIRDEFITMWRDLLSKPAA
                     APRTWVLRDFHSPNLIWLDERSGVAKVGIIDFQDAVLGPAAYDLVSLLQDARIDVPEQ
                     LELALLARYIKARRADDDGFDPAGFAELYAIMSAQRNTRLLGTFARLNRRDGKPQYLR
                     HQPRIWTYLNRSLAHPLLPHVRAWYSAHVPPPAR"
     gene            1026..1343
                     /locus_tag="VN968_01515"
     CDS             1026..1343
                     /locus_tag="VN968_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXL27960.1"
                     /translation="MVAERRKGERVIFERGISAHMMGIDGTWRRDCTMEDVSETGAKL
                     SVEGSVEGLHLKEFFLLLSSTGLAYRRCELSWVNGDQIGVNFLRPGDKKKKARRDAQD
                     AEV"
     gene            1484..2206
                     /locus_tag="VN968_01520"
     CDS             1484..2206
                     /locus_tag="VN968_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXL27961.1"
                     /translation="MSVKPSKAMVLAAGLGVRMRPLTDRMPKPLVRVAGRALLDHVLD
                     KLGDAGVSEAVVNVHYLPDQIIDHTAARMRPRVIISDERDVVLGTGGGVVKALPMLGD
                     APFFHVNADTLWIDGVRPNLGRLAEAFDPARMDILLLMAPTTSSIGYGGRGDYAMLPD
                     GALRKRKEHQVVPFVYAGVAIMSPSLFADAPTGEFSLTKLFDSANEQERLFGLRLDGV
                     WMHVGTPDAVHAAEEAFLASVA"
     gene            2390..>4079
                     /gene="addB"
                     /locus_tag="VN968_01525"
     CDS             2390..>4079
                     /gene="addB"
                     /locus_tag="VN968_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HXL27962.1"
                     /translation="MRVFSVPLSAPLLRAVIAALIDGKLVAGFEARAHPEKLAEATLY
                     LPTRRAGRMAREIFLDELKADAAVLPRIVALGDIDEDELAFAEQAEQYGGAAPLDIPP
                     KLGELDRRLTLARLVAAWARSPVSAPLVVGGPASTLALAGDLARLMDDMVTRGVGWEA
                     LDGLVPDRLDKYWQHSLEFLQIARRAWPAHLVEIEKIEPAARRDRLIEAEARRLTAHH
                     EGPVIAAGSTGSMPATAKFLHAVATLPQGAVVLPGLDTDLDDDAWQLIGGIRDAQGRL
                     TTASAPNHPQFAMHALLDRLGIKRGDVEVLGEPAPLGRDVLVSEAMRPSNATAEWHRR
                     LGQPDIVAKISAGLTNLAVVEAANPEMEALAIAVAMREARHLDKSAALVTPDRALARR
                     VMAALGRWNLEFADSGGDGLMDTSAGIFARLTAEAAARQLEPPTLLALLKHPLCRLGG
                     ARGAFQDTIETLELALLRGTRPQAGSSGLARDFDRFRLELEKLKRRETSSLHGSEPRT
                     RLRNDELDRAGQLIKLLHAALSPLESLNASKPYDFGELAERHRNALMDLSRDQHG"
BASE COUNT          701 a         1299 c         1331 g          748 t
ORIGIN      
        1 catcctgatg aactcgccgc ggcggccgga cgggccggcg gtctatgacg gaaaatcata
       61 cagtgcggcg gttcatctgg ccgaggacgt cacgccgttt gtcgcgattg caaacggcct
      121 gcgcgagcgc ggcttctcgg ccccaacaat ccatcatgcc gacctcgata ccggattcct
      181 gatcaccgag gatttcggca gcgccggcct tgtcgaaggc gatccatcgg ggccgaccgt
      241 ggagcgatat caagttgcga ccgacttgct ggcggcgctg caccgccagg tgttgccctt
      301 cattctgcct gtggtgcccg acagcaccta cgccataccc actttcgaca ccgatgccct
      361 gctgatcgag gccggattga tgctcgaatg gtatctgccc gaccgcggcg ccaaaccggc
      421 cgacgagata cgcgacgaat tcatcacgat gtggcgcgat ttgctgagca agcccgcggc
      481 ggcaccaagg acctgggtgc tgcgcgactt tcattccccc aacctgatct ggctcgacga
      541 acggtctggt gttgcgaaag tcggcatcat cgacttccag gacgcggtgc tgggaccggc
      601 cgcctatgat ctggtgtcgc tgctgcagga cgcgcggatc gacgtgccgg agcagctcga
      661 gctcgcgctt ctggcgcgtt acatcaaggc gcggcgcgcc gacgacgacg gcttcgaccc
      721 cgccggtttc gccgagctgt acgccatcat gtcggcgcag cgtaacacgc ggctgctcgg
      781 cacctttgcg cggctcaacc gccgcgacgg caagccgcaa tatctccgcc atcagccccg
      841 aatctggacc tatctcaacc gttcgctggc tcaccccctg ctcccgcacg tccgggcctg
      901 gtattcggcc cacgtccccc cgccggcccg gtagttttac cagttgttag ccacctccgg
      961 ttaatctgcg ccggaaaagt gcaccgcagc gcgcgagccg gctgctttgg gagtgagggc
     1021 aaccgatggt ggcagaacgg cgcaagggcg agcgcgtcat attcgaacgc ggcatcagtg
     1081 cgcatatgat gggcatcgat ggaacctggc ggcgcgattg caccatggaa gacgtttccg
     1141 aaaccggcgc gaagctctcc gtggaaggat ccgtcgaggg gctgcatctg aaggaatttt
     1201 tcctgctgct gtcgtccacc ggattagctt accgccgctg cgagctatcc tgggtcaacg
     1261 gcgaccagat cggcgtcaat ttcctcaggc ccggcgacaa gaagaaaaag gcacggcgcg
     1321 atgcgcaaga cgccgaagtc tgatctcgcc ggcatgatac tcgtcgcatg actgtcatct
     1381 cggacggcta tggcatcggc gatcgatgcg gcgcgccaat cgccgtgata tgatccgcgc
     1441 cacccagcgc ctccaacaac aacggttcgt gaattcccca aaaatgtcgg tcaaacccag
     1501 caaagccatg gtccttgccg ccggtctcgg cgtgcgcatg cgtcccctga cggaccggat
     1561 gccgaagccg ctggtgcggg tggcgggccg ggcgctgctc gatcatgtgc tcgataaact
     1621 cggcgacgcc ggcgtcagcg aagccgtcgt caatgtgcac tatctgcccg accagatcat
     1681 cgatcacact gccgcgcgca tgcgaccccg cgtgatcatc tccgatgagc gtgacgtcgt
     1741 gctcggcacc ggtggcggcg tcgtcaaggc gctgcctatg ctcggcgatg ccccattttt
     1801 tcatgtcaat gcggatacct tgtggatcga cggtgtgcgg cccaaccttg ggcggttggc
     1861 cgaagccttc gatcccgcgc gcatggacat cctgctcctg atggcgccga ccacgagcag
     1921 cattggttat ggcggacgcg gcgattacgc gatgctgccc gatggcgcgc tgcgcaagcg
     1981 caaggagcat caggtggtgc cattcgtcta tgcgggggtc gcgatcatgt cgccatcctt
     2041 gttcgccgat gccccaacgg gtgaattctc gctgacaaag ttgttcgatt ctgccaacga
     2101 gcaggagcgg ctgttcggcc tgcggctgga cggcgtgtgg atgcatgtcg gaacccctga
     2161 cgccgttcat gccgcggaag aggcctttct cgccagcgtc gcgtgatcgc atccgcggct
     2221 ttttgttgtc tttgccgggc ttgacctggc aatccatctt cttttgaact gaatccttat
     2281 ggatggatgc gcgggtcagg cctgcgcatg acacgtaggt tacgaattag aaatcgtacc
     2341 gcaaatttgc ccatgccatg atgtcgcctg accacgaatc aggaagccca tgcgcgtttt
     2401 cagcgttccc ctatcagcgc cgttgttacg tgccgtcatc gccgcgctga tcgatggcaa
     2461 gcttgtcgct ggcttcgagg cgcgcgcgca tccggaaaaa ctggcggagg caacgctcta
     2521 tttgccgacc cggcgcgccg gacgcatggc gcgggaaatc tttctcgacg aactgaaagc
     2581 tgacgcggcg gtgctgccgc ggattgtcgc gctcggcgac atcgacgagg acgaactggc
     2641 gttcgccgag caggccgaac aatatggcgg agcggcaccg ctggacattc cgccgaagct
     2701 tggcgagctc gaccgtcggc tcacgctggc aagactggtc gcggcctggg ccaggagtcc
     2761 cgtgtcggcg ccactcgtcg ttggcggccc ggcgtcgaca ctggcgttgg cgggcgatct
     2821 ggcgcgcctg atggacgaca tggtcacccg cggcgtcggc tgggaagcgc tcgacgggtt
     2881 ggtgccggat cgactggaca aatattggca gcactcgctc gaattcctgc agatcgcgcg
     2941 gcgggcgtgg ccggcgcatc ttgtggaaat cgagaagatc gagccggcgg cgcggcgcga
     3001 ccgcttgatc gaggcggaag ccaggcgcct gaccgcccat catgagggcc cggtgatcgc
     3061 ggcgggatcg accggttcga tgccggcgac cgcgaaattc ctccacgccg tggccacgct
     3121 gccgcaaggc gcggtggtgt tgccgggtct cgacaccgat ctcgacgacg atgcgtggca
     3181 actgatcggt gggatcaggg acgcgcaagg caggttgacc acggcttcgg cgccgaacca
     3241 tccgcagttc gccatgcacg cgctgcttga tcgactggga atcaagcgcg gcgatgtcga
     3301 ggttctgggc gagccggcgc cgcttgggcg cgatgtgctg gtgtccgagg cgatgcggcc
     3361 ttcgaatgcg accgcggagt ggcatcgacg gctgggccag cctgacatcg tggcgaagat
     3421 ttccgccggc ctgactaatc tcgccgtcgt cgaagccgcc aatccggaga tggaagcgct
     3481 ggcgatcgcg gtggcaatgc gcgaggcgcg gcatctggac aaatcggcgg cgctggtgac
     3541 gccggatcgc gcgctggcgc ggcgcgtgat ggccgcactc ggccgctgga atctcgaatt
     3601 cgccgattcc ggcggcgacg gcttgatgga cacgtcggcg ggaatttttg cccggctcac
     3661 cgcggaggcg gccgcgcggc agctcgagcc gccgaccctg ctggcgctcc tgaagcaccc
     3721 gctctgccgg ctcggcggcg cgcgcggcgc gttccaggac acgatcgaaa cgctggagct
     3781 ggctctgctg cgcggcacgc ggccacaggc cggaagcagt ggcctcgcgc gggattttga
     3841 tcgcttccgt cttgagctcg aaaagctcaa gcgccgcgag acgtcctcgc tgcatggctc
     3901 cgagccgagg accaggcttc gaaatgacga actcgatcga gcgggtcagc tgatcaagct
     3961 tttgcacgcc gcgctttcgc cgcttgaaag cctaaacgcc tcgaagccgt acgactttgg
     4021 cgagctggcg gaacgccatc gcaacgccct gatggatctg tcccgcgacc agcatggtg
//
LOCUS       DATNOI010000043         2026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1043915,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000043 DATNOI010000000
VERSION     DATNOI010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043915"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1095)
                     /locus_tag="VN968_01530"
     CDS             complement(205..1095)
                     /locus_tag="VN968_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL27963.1"
                     /translation="MKHPCWQTAGIGIAAAFAFAMMAGINPAVAQGAIQVTKCAPGAL
                     ASKYPSVAGKTFRIGQDGESPPYSFRDSKDFNNVIGFDADLARAVLACLGAKIEFKIG
                     GWSGLLPAVIAGQSDAMWDNLYYTAERAKLVDYISYMTAGTGILAKKGNPKKIHSVAD
                     ACGTSATAVLGTVEEAAFRDQSKKCVAEGKAEINIFTAPDIPAATRLMQTGRADLFMN
                     DLALIDILATDSNGQLERAVKIVSNFKIGIAIKKGRDDVVAAFHDALVVVQNDGTQKA
                     LYEKYKIDPSLALPSEILKQ"
     gene            complement(1724..>2026)
                     /locus_tag="VN968_01535"
     CDS             complement(1724..>2026)
                     /locus_tag="VN968_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27964.1"
                     /translation="RVAGMDFSRATYRDLAVQTFMAIPYVAGSYQTVANYLDGLAEQG
                     LAGVCFIFPDYEHDLQRLIENVLPRLKYRPLRPPDGAGDPGGRSDAPPVPVAVGRR"
BASE COUNT          375 a          630 c          624 g          397 t
ORIGIN      
        1 ctccgctcgc gaggtcgctc agggtttgcg cgtaaacggc cgatgcgatg ccgtcgccag
       61 gtccagtcga gccgtgtctc atcaaaagga cacacgccac cgccgccgcg agcgtgagcc
      121 tcccgcccca ccgcccgatc acactgccac cctgcctgga attgcacggc cccggattgt
      181 cgcccatcac ggtctaaagg cgcgtcattg cttgaggatt tccgacggca gggcgagcga
      241 cggatcgatc ttgtacttct cgtatagcgc cttctgggtg ccgtcgttct gcacaacgac
      301 cagcgcatca tggaatgcgg cgacgacatc gtcgcgcccc tttttgatcg cgatcccgat
      361 cttgaagttg gaaacgatct tcacggcgcg ttcgagctgg ccgttgctgt ccgtcgccag
      421 aatgtcgatc agggcgagat cgttcatgaa caggtcggcg cggccggtct gcataagccg
      481 cgttgcggcg ggaatatccg gcgccgtgaa aatgttgatc tcggccttgc cttcggccac
      541 gcacttcttg ctttggtcgc ggaaggccgc ctcctcgacc gttccaagga ctgcggtcgc
      601 cgacgtgccg caggcgtccg cgactgaatg gatcttcttg ggattcccct tcttggcgag
      661 aatgccggtg ccggccgtca tataactgat gtagtcgacg agcttcgccc gctcggcggt
      721 gtagtagagg ttgtcccaca tcgcatccga ttgtccggcg atgacggcgg gcaacagtcc
      781 ggaccagccg ccgatcttga attcgatttt cgcgcccagg catgccagca cggcacgcgc
      841 aagatcagcg tcgaagccga tgacattgtt gaagtccttg gagtcgcgga aactgtatgg
      901 cgggctttcg ccgtcctggc cgatgcggaa ggtttttccc gcaacgcttg ggtatttgct
      961 cgcgagcgca cccggcgcgc atttcgtcac ttgaatcgcc ccttgcgcga cggcaggatt
     1021 gatgccagcc atcatggcga acgcgaatgc cgccgcaatc ccgatcccgg cagtctgcca
     1081 acaaggatgt ttcatagctg ccgccctcct gtgccttaag ctttatcgcg acgcctgcgc
     1141 ggccgttgcc gtgcaatggc ttgcacgaaa tgaaagcaag ttgcgtgcca tggaatcgcg
     1201 ggaccgccgg ccggagtaaa agcgccgcgg aacgtccagt ggacggtatc gcctcggctg
     1261 cggagcttgg gcagccgctc ggcccttgcc aaagttcgcg ccatcgaacg atgaacgggc
     1321 aggcgaagag ttatttccgg ccggcggcga agggatggat tttcatccaa cgagagcgaa
     1381 tcccattctt atggaatcac acaggcatgt catcgcccgc gaaaaagcgt gtgaaataat
     1441 ctgccctcat cctgaggagg ccgccctcat gggcggccgt ctcgaaggat ggcagcaggc
     1501 atggccgagc aggtggccat ccttcgagac gcgccgctaa gaagcggcgc tcctcaggat
     1561 gagggctttc aatttcttca cacgctcgaa agcgggcgat ccagtaaccc ccggcgatgc
     1621 tgcggagtac tggattcccg ctttcgcggg aatgacaccg cagagcgggt ggtcgccgtt
     1681 tgcttccatt ttatcggcac gctctagtca acaccgcgtt tgatcatcgc cgcccgaccg
     1741 cgaccggaac cggcggcgcg tcgctgcgac cgcccgggtc gccggcacca tccggtggcc
     1801 gcagggggcg atatttgagg cgtggcagca cgttttcgat caggcgctgc agatcatgct
     1861 catagtcggg aaaaatgaag cagacgccgg ccagcccctg ttccgccaag ccgtcgagat
     1921 agttcgctac ggtctggtac gacccggcca cgtaaggaat cgccatgaag gtctgaactg
     1981 cgaggtcgcg gtaggtcgcg cgcgagaaat ccatgccggc aacgcg
//
LOCUS       DATNOI010000044         3817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1752924,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000044 DATNOI010000000
VERSION     DATNOI010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752924"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..641)
                     /locus_tag="VN968_01540"
     CDS             complement(48..641)
                     /locus_tag="VN968_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXL27965.1"
                     /translation="MAALCLGGRTAHAQAAPVTYWTPGWPLGFGGSPIVDQSSDTYGN
                     FPSFDGSDARGGGSYMRYNFPNGWFVGGEGGGMGLSMNGINQNGAFGNFGSLYYQGVQ
                     FGYNFRSAGGLPVTVYAGFDTLNYKSGIGGPFASFDTTSGTLPGYSAHAGLEFQPAPN
                     VSLSLGFGYTQQSGRIDSDINSLSLPGASPFAASGRR"
     gene            complement(868..1590)
                     /locus_tag="VN968_01545"
     CDS             complement(868..1590)
                     /locus_tag="VN968_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXL27966.1"
                     /translation="MSVHPTKAMVLAAGLGVRMRPLTDRIPKPLVRVAGRALLDHVLD
                     KLGNAGAGEAVVNVHYLPDQIIDHVAARARPRVIISDERDQVLGTGGGVVKALPLLGP
                     APFFLVNADTLWIDGVRPNLARLAEAFEPSRMDILLLMAPTASSIGYGGRGDYAMLPD
                     GALRKRRELQVVPFVYAGAAIMSPSLFNDAPSDEFSLTRMFDRANEQERLFGLRLDGV
                     WMHVGTPDAVDAAEEAFLASVA"
     gene            complement(1728..2051)
                     /locus_tag="VN968_01550"
     CDS             complement(1728..2051)
                     /locus_tag="VN968_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXL27967.1"
                     /translation="MVAERRKGERVAFERGIGAHMMGIDGTWRRQCTMEDVSESGAKL
                     TVEGSVEGLHLKEFFLLLSSTGLAYRRCELAWVNGDQIGVNFLKTGEKKKKTARRGSP
                     QAAEV"
     gene            complement(2136..3659)
                     /gene="tsaE"
                     /locus_tag="VN968_01555"
     CDS             complement(2136..3659)
                     /gene="tsaE"
                     /locus_tag="VN968_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXL27968.1"
                     /translation="MTDPTTFSLALSNETATAHLMADLALLIGPGDVITLSGDLGAGK
                     TSAARAMIQYLADDDALEVPSPTFTLAQAYDVPPFPLLHADLYRINDPAELEEIGLSP
                     LPEGMVALIEWPERAAGVLPPDRIDIALSHRAALGSTARAAEITGYGKAAAQVARLKA
                     LREFLDAAGYVEAKRQRMPGDASIRSYARLIRDDGVVILMNFPRRPDGQALYDGKSYS
                     AAVHLAEDVKPWVAIDNALRARGFSAPSIHHADLDSGFLISEDFGVAGLVEGDPPAPI
                     AERYEAATDLLAALHRQALPETLPLSPQLTYAIPTFDTDALLIEIGLMLEWYLPDRGA
                     ELTEQMRADFITMWRDLLSKPAAAGKTWVLRDFHSPNLIWLGERSDIARVGIIDFQDA
                     VLGPPAYDLVSLLQDARVDVPEQLELALLTRYIRARRASDDAFDPAQYAELYAVMSAQ
                     RNTRLLGTFARLNRRDGKPQYLRHQPRIWTYLTRSLAHPVLSRAREWYSANVPPPVP"
     gene            complement(3656..>3817)
                     /locus_tag="VN968_01560"
     CDS             complement(3656..>3817)
                     /locus_tag="VN968_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL27969.1"
                     /translation="GSRHRGAGLGLSLVRSFVELHGGKVRVDSVVGKGTTVTCDFPSD
                     QAAHRNAAE"
BASE COUNT          725 a         1172 c         1254 g          666 t
ORIGIN      
        1 agcaatcgag caaacgccgc aagatggtca atcgcttccc tttttactta gcggcgaccg
       61 ctggcggcaa acggagaagc gccgggcagc gacagcgaat tgatgtcgct atcgatgcgc
      121 cccgactgtt gggtgtagcc gaacccgagt gacaggctga cattcggcgc cggctggaac
      181 tcgaggcccg catgcgcgct atagccgggc agtgtgccgg acgtggtgtc gaaggaagca
      241 aaagggccac cgatgccgga tttataattc aaggtgtcga aaccggcata gacagtgacg
      301 ggtaacccgc ccgcgcttcg gaagttgtag ccgaactgca cgccttggta ataaagcgag
      361 ccgaagttgc cgaacgctcc attctggttg atgccgttca tgctcaaacc catgccgccg
      421 ccttcgccgc cgacaaacca gccgttcgga aaattgtagc gcatatagga gccgccgcct
      481 cgggcgtcac tgccgtcaaa gcttggaaag ttgccgtaag tgtccgagct ctggtcgacg
      541 attggactgc caccgaagcc gaggggccag cctggggtcc aatatgtcac aggcgcggct
      601 tgggcatggg ccgttcgccc gcccagacag agcgccgcca atacgagtgc gagacctaat
      661 ccgcttgaga agatggacat ccgcgctacc gtcaagtgtc ctgaaactat acggtctgga
      721 tatcaagaac gccagtgcgg cgtcgccgag ggtcgcgccg tcattccggg gcgacgcgaa
      781 gcgtcgaacc cggaatctcg agattccccg atgtgcaatt gcacatctgg ggttcgcgtc
      841 ttcgacgcgc cccggaatga cgaagcgtta cgcgacgctg gcgaggaagg cctcttccgc
      901 cgcgtctacc gcgtcggggg ttccgacatg catccacacg ccgtcgagcc gaaggccgaa
      961 cagccgctcc tgttcgttgg cgcgatcgaa catcctggtc agcgaaaact cgtccgatgg
     1021 ggcatcgtta aacagtgacg gcgacatgat cgcagcgccg gcatagacga acggcacgac
     1081 ctgaagttcc ctgcgcttgc gcaacgcgcc gtcgggcagc atggcgtaat cgccgcgtcc
     1141 gccataaccg atgctgctcg ccgtcggcgc catcaacagc aggatatcca tgcgcgaggg
     1201 ctcgaaggct tcggcgagcc gggccagatt gggccgcacg ccatcgatcc acagggtgtc
     1261 tgcgttgaca aggaagaacg gagccgggcc aagcaaaggc agcgccttga cgacgccgcc
     1321 gccggtgccg agcacctggt cgcgctcgtc cgagatgatc acgcggggcc gggcgcgggc
     1381 ggcgacgtga tcgatgatct ggtcgggcag atagtgcaca ttgacgacag cttcgccggc
     1441 gcctgcgttg ccgagcttgt cgagcacatg atcgagcagc gcgcggccgg ccacgcgcac
     1501 cagcggtttt ggaatccggt cggtcaaggg gcgcatgcgg acgccaagtc cggcggcgag
     1561 gaccatggct ttggtggggt ggacggacat ttttgggatt tctcgaaccg tgtttgtggt
     1621 ggcgtatgtt tttgccccga tcatatcacg gggattcgcg gaggctaccg cccgtcgatg
     1681 ccataaccgt cgggcatgac ggttgtgcga ccgggaaact ttcgcgctca gacttccgcg
     1741 gcctgcggcg aaccgcgccg agccgtcttt tttttctttt cgccggtttt gaggaaattg
     1801 acgccgatct gatcgccgtt gacccaggcc agttcgcagc ggcggtacgc caaaccggtg
     1861 gacgacagaa gcaggaagaa ttccttgaga tgcaacccct ctacggaacc ttcgacggtg
     1921 agtttcgcgc cgctttccga aacgtcttcc atggtgcatt ggcgccgcca ggtgccatcg
     1981 atgcccatca tgtgcgcgcc gatgccgcgt tcgaacgcga cgcgctcgcc cttgcgccgt
     2041 tctgccacca tggtcccgcc ctcaagcagc ccccgagctt ccatgcgcga gggctggcaa
     2101 gagattaaag cggggttgac taacaaccgg taaactcagg gaaccggcgg ggggacgttg
     2161 gctgaatacc actcgcgcgc gcgcgacagc accggatgtg ccagcgaacg cgtgaggtag
     2221 gtccagattc gcggctggtg gcgcagatat tgcggcttgc catcgcggcg gttgagccgt
     2281 gcaaaggtac cgagtagccg cgtgttgcgc tgcgccgaca tgacggcata gagctcggca
     2341 tattgagccg gatcgaaggc atcatcagag gcgcgccgcg ccctgatata gcgcgtcagc
     2401 agcgcaagct cgagctgctc gggcacatcg acccgcgcgt cctgcagcag cgataccaga
     2461 tcgtaggcgg gcggccccag cacggcgtcc tggaaatcga tgatgcccac tctcgcaatg
     2521 tcggaccgtt cgccgagcca gatcaggttg ggggaatgaa agtcgcgcaa cacccaggtt
     2581 ttgccggcag cagcgggctt gctcagcaaa tcgcgccaca tcgtgatgaa atcggcgcgc
     2641 atctgctcgg tcaactccgc cccgcgatcc ggcagatacc actcgagcat caatccgatc
     2701 tcgatcaaaa gcgcgtcggt gtcgaaggtc gggatcgcat aggtgagttg cggcgaaagc
     2761 ggcagggttt cgggcaaggc ttggcgatgc agcgccgcga gcaggtcggt tgcggcctca
     2821 taacgttcgg cgatcggtgc gggtggatcg ccttcgacga gacccgcgac gccgaaatcc
     2881 tcgctgatca gaaatccgct gtcgaggtcg gcgtgatgga tggatggcgc cgagaagccg
     2941 cgggcgcgca gagcgttgtc gatcgcgacc cagggtttga catcctcggc cagatgcacg
     3001 gccgcgctgt atgacttacc gtcatagagc gcctggccgt cggggcggcg cggaaagttc
     3061 atcaggatga ccaccccatc gtcgcggatg agccgcgcat aggaacggat cgacgcgtcg
     3121 cccggcatgc gctgccgttt cgcctcgaca tagcccgccg cgtcgaggaa ctcgcgcagc
     3181 gccttgagtc gcgcgacttg cgcagccgct ttgccgtagc cggtgatttc ggcggcgcgg
     3241 gcggtcgatc ccagcgccgc acgatggctc agcgcgatgt cgatccggtc tggcggaagc
     3301 acgccggctg cgcgctccgg ccattcgatc agcgcgacca tgccctcggg caatggcgac
     3361 aagccgatct cttccagttc cgccggatcg ttgatgcggt agagatctgc gtgaaggagc
     3421 ggaaatggcg gcacatcgta ggcttgcgca agcgtaaacg tcgggctcgg cacctcgagc
     3481 gcatcgtcgt cggcgaggta ctggatcatc gcccgcgcgg cggaggtttt gccggcgcca
     3541 agatcgccgg aaagggtaat gacatcgccc ggtccgatca gcagcgcaag atcggccatc
     3601 aaatgcgcgg ttgctgtctc gttcgatagc gccagcgaga atgtggtggg atcggtcatt
     3661 cggcggcgtt gcgatgcgcg gcctgatcgc tcggaaagtc gcacgtcacc gtcgtgccct
     3721 tgccgacgac cgaatcgacg cgcaccttgc ctccgtgcag ttcgacaaag gagcgcacca
     3781 gcgacaggcc gaggccggcg ccgcgatgcc gcgatcc
//
LOCUS       DATNOI010000045         5216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2282885,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000045 DATNOI010000000
VERSION     DATNOI010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2282885"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(702..1643)
                     /locus_tag="VN968_01565"
     CDS             complement(702..1643)
                     /locus_tag="VN968_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HXL27970.1"
                     /translation="MNAAPSFKRIALIGFGLIGGSIARAAREQGLAGEIVTTARSAKT
                     RARVSELGIVDRVVETNAEAVDNADLVILCIPVGACGAVAQEIAPYLKPGAIVSDVGS
                     VKGAVAREMAPHLPAGVHFVPAHPVAGTEHSGPDSGFAELFINRWCILTPPDGTDPDA
                     VAKLGAFWTALGAKVETMTPDHHDLVLAITSHLPHLIAYTIVGTADELGQVTSSEVMK
                     FSAGGFRDFTRIAASDPTMWRDVFLANKEAVLEMLGTFNEDLSKPTRAIRRNDGEALF
                     EHFTRTRAIRRGIVEIGQDSAAPDFGRPHAQLGKKAD"
     gene            complement(1640..2737)
                     /locus_tag="VN968_01570"
     CDS             complement(1640..2737)
                     /locus_tag="VN968_01570"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HXL27971.1"
                     /translation="MSRPVPNPGILEIAPYTPGKSPVPEPGRKVFKLSANETPFGPSP
                     KAIAVYKEAAAHLEDYPEGTSRVLREAIGRAFGLDPDRIICGAGSDEILNLLAHTYLS
                     HGDEAISTTHGFLVYPIATMANGATNVVAPETNFTADVDAILKLVSPRTKLVWLANPN
                     NPTGTYLPFDEVKRLRAGLPPHVLLVLDAAYSDYVSRNDYEIGIELVATTENTVMTHT
                     FSKIHGLAALRIGWMFGPAHIVDAVNRIRGPFNVSTPAMLAAVAAIEDTAHVQMSRAH
                     TEKWRNWLTEQIGKLGLMVTPSVANFVLIHFPLDKGKTAADADAFLTKRGLVLRGLNN
                     YGLPHALRMTIGTEEANRLVVDGLRDFTARK"
     gene            complement(2764..3615)
                     /locus_tag="VN968_01575"
     CDS             complement(2764..3615)
                     /locus_tag="VN968_01575"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HXL27972.1"
                     /translation="MSKAPPAPPSLAVLRKEIDAIDEQVHNLLMARGDIIDRLISVKQ
                     TQEVGSAFRPAREADMMRRLVQRHRGILPLDTVESIWRVIISTFTYVQAPFSVHADIS
                     LGESAMRDSARFHFGFTVPYVSHFSAQAAVDAVATSKGDLALVSATSSRTPWWIALEA
                     DGAPKIIARLPFVERADHPAALPVFVVSRVADSAMVTEVEMWSVRVSGWNAEIARALS
                     PLAEIVAVPDTAFDGAALLVSVAGHSSLEKIRSALIEAGASVRSSALVGSHATRYTVP
                     PNGAAKA"
     regulatory      3777..3856
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            3943..5151
                     /locus_tag="VN968_01580"
     CDS             3943..5151
                     /locus_tag="VN968_01580"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HXL27973.1"
                     /translation="MTNVQSIPSPAIHSEERAHEADHPSSLVSAFGADQPLRLDCGVD
                     LSPFQIAYQTYGQLNGERSNAILIFHALTGDQHVANVHPVTGKPGWWETMVGPGRPLD
                     TDRYFIICSNVIGGCMGSTGPASTNPATGKLWGLDFPMITIPDMVRAQAMLIDRLGIE
                     TLLCVTGGSMGGMQALQWTAAYPERVFSALAVACSTRHSAQNIAFHELGRQAVMADPD
                     WRGGRYFEEGTHPHRGLGVARMAGHITYLSDAALHRKFGRRMQDRDLPTFSFDADFQV
                     ESYLRYQGSSFVERFDANSYLYLTRAMDYFDIAADHSGVLARAFRGIQTRFCVVSFTS
                     DWLFPTSESRALVHALNASSARVSFAEIETDRGHDAFLLDVPEFFDISRAFLQSAGTA
                     RGLAKGGRPT"
BASE COUNT          944 a         1634 c         1711 g          927 t
ORIGIN      
        1 cggtgaagtc gtgtggtcct gacgcctcga cgctggcgtc aagtcttgcg gaggctcgtt
       61 cggctcaacc gggctggaca aaaccttcaa tccgcaaggc gacggtggca aaagagcccg
      121 atcgccgggg agagcacgaa gtaagccgta aaaccattgc gtgcgggaac gtcgggtgat
      181 tccggtgtac tcgtatactc gtgtgcgttc taccaatacc aagtgcacac gaggccgcgg
      241 gtgcaacggg catccggcgt tccccacgcc ctctttgggc ggaagattca tcaacggctc
      301 gggcgcatcg cgcggcgagg tcgcgaagtt gtgtctacga agatcattca aagcgatcgc
      361 gccacacttt cagtcgtcat cgcccgcgaa agagcctgtg aagaatgccg acctcatcct
      421 gaggagcttg cgaagcaagc gtctcgaagg atggacacaa catacggact cacggccatc
      481 cttcgagacg cgccgcaaga gcgcgctcct caggatgaag tcggagatat atttcacaat
      541 cgcgaaagct ggcgatccag tattccgagg cgtcagttat ggaaacgcaa gtctgcgggg
      601 tactggatac tccgctttcg cggagtatga cggtgttgcg aggagcgact tgtccgccgg
      661 agctcaacga gcgaaggcgg atgcaacatg gcagtgctgt ctcaatccgc cttcttcccc
      721 aactgcgcat gcggacggcc gaagtcgggt gcggcggagt cctggccgat ctcgacgatg
      781 ccgcggcgga tggcgcgggt gcgggtgaag tgctcgaaca gcgcctcgcc gtcgttgcgg
      841 cggatcgcgc gggtgggctt ggagagatcc tcgttgaagg tgccgagcat ctccagcacc
      901 gcttccttgt tggccaggaa gacgtcgcgc cacatggtgg gatcggaggc ggcgatgcgg
      961 gtgaagtcgc gaaagccgcc ggccgagaac ttcatcactt ccgacgacgt cacctggccg
     1021 agttcgtccg cggtgccgac gatggtgtag gcgatcagat gcggcaggtg gctggtgatc
     1081 gccagcacca ggtcgtgatg gtccggcgtc atggtctcga ccttggcgcc cagcgcggtc
     1141 cagaacgcgc cgagcttcgc gaccgcgtcg ggatcggtgc catcaggcgg agtgaggatg
     1201 caccagcgat tgatgaacaa ttcggcgaag ccggaatcgg gaccggagtg ctcggtgccg
     1261 gcgacgggat gcgctggcac gaaatggacg ccggccggca gatgcggcgc catctcccgc
     1321 gcgaccgcgc ccttgaccga gccgacatcg gagacgatcg cgcccggctt gagatatggc
     1381 gcgatttcct gcgcgaccgc gccgcaggcg ccgaccggga tgcacaggat gacgagatcg
     1441 gcgttatcaa ccgcttccgc attggtctcg accacgcggt cgacaatgcc gagttccgag
     1501 acccgcgcgc gggtcttcgc cgagcgcgcg gtggtgacga tttctcccgc caacccctgc
     1561 tcacgcgcgg cgcgcgcgat cgaaccgccg atcaggccga agccgatcag cgcgatccgt
     1621 ttgaatgacg gcgcggcgtt cacttgcgcg ccgtgaagtc gcgcaagcca tcgaccacca
     1681 gccgattggc ctcctcggtg ccgatggtca tgcgcaaagc gtgcggcagc ccgtaattgt
     1741 tcagtccgcg cagcaccagc ccgcgtttgg tcaggaacgc gtcggcgtcg gccgcggtct
     1801 tgcccttgtc gagcgggaaa tggatcagca cgaaattcgc cacgctcggc gtcaccatga
     1861 ggccaagctt gccgatctgc tcggtcagcc agttgcgcca cttttcggtg tgcgccctcg
     1921 acatctggac atgcgcggtg tcctcgatcg ccgcgaccgc cgccagcatc gccggcgtcg
     1981 agacgttgaa gggtccgcgg atccggttga ccgcatcgac aatatgggcc ggcccgaaca
     2041 tccagccgat ccgcagcgcc gcaaggccgt gaatcttgga aaacgtgtgg gtcatcacgg
     2101 tgttttcggt ggtcgccacc agttcgatgc cgatctcgta gtcgttgcgc gatacgtaat
     2161 ccgaataggc ggcgtcgagc accagcagca catgcggcgg caatcccgcg cgcagccgct
     2221 tgacctcgtc gaacggcaga taggtgcccg tagggttgtt cggattggcg agccacacga
     2281 gcttggtgcg cggcgagacc agcttgagga tcgcatcgac gtcggcggta aagttggtct
     2341 cgggcgcgac gacgttagtg gcgccgttcg ccatggtcgc gatcggatac accagaaagc
     2401 cgtgggtggt gctgatggcc tcgtcgccgt ggctgagata ggtatgcgcc agcaagttga
     2461 ggatttcatc ggagccggcg ccgcagatga tgcggtcggg atcgagcccg aaggcgcggc
     2521 cgatcgcctc gcgcagcacg cgcgacgtac cttccggata atcctccaga tgcgccgccg
     2581 cctccttgta gaccgcgatc gccttcggcg aaggtccgaa tggcgtctcg ttggccgaca
     2641 gcttgaacac cttgcggccc ggctcgggca ccgggctctt gcccggcgtg tagggcgcaa
     2701 tctcgagaat gccgggattc ggcacggggc gggacatctt caactccgga ttatcggggg
     2761 ctctcaagct ttcgctgccc cgttcggggg caccgtatag cgcgttgcgt ggctgccgac
     2821 gagagccgat gagcgcaccg atgcaccggc ctcgatcagg gccgatctga tcttttcaag
     2881 gctgctgtgg cctgccaccg aaaccagcag cgccgcgccg tcaaaggcgg tatccggcac
     2941 cgcgacgatt tcggccagcg gcgacagcgc gcgcgcgatc tcggcgttcc agccggacac
     3001 ccgcacgctc cacatctcga cttcggtcac catggcgctg tcggcaaccc gcgacaccac
     3061 gaataccggc agtgccgccg gatggtcggc gcgctcgacg aacggcagcc gcgcgatgat
     3121 cttcggcgca ccgtccgctt cgagcgcgat ccaccacggg gtgcggctgg aagtggcgga
     3181 gaccagcgcc agatcgccct tcgacgtggc caccgcgtcg accgcggcct gcgcgctgaa
     3241 atgcgagacg tagggaacgg taaagccgaa atgaaaccgc gccgaatccc gcatcgcgga
     3301 ttcgccaagc gaaatatccg catgcaccga gaacggcgcc tggacatagg tgaaggtcga
     3361 gatgatgacg cgccagatgc tttcgacggt gtcgagcggc aggatgccgc gatggcgctg
     3421 caccagccgg cgcatcatgt cggcctcgcg tgcgggacga aacgccgagc cgacctcctg
     3481 ggtctgcttc accgaaatca gccgatcgat gatgtcgccg cgcgccatca gcaggttgtg
     3541 gacctgctcg tcgatagcgt cgatctcttt gcgcagcacc gccagcgagg gcggagcggg
     3601 gggtgccttg gacatgtcat accctgacgg ccggacagcc tgatgccgtc ggcagataag
     3661 gccattgatt agaaaagacg gccgccgaaa gcaaagagaa acgtcagcgc ctgacagtcg
     3721 cggccctggt taaccatggg cctgccttga cgacaggccc gcgcaggact agcttgagct
     3781 cacttcgtgg tcatttgagc cggccggctt gcagccacgt taaacaactc gctaaacagg
     3841 ccggggacag atgtgatccc ggccgaacct tatgttcagg ccgggttttt ttatggcctg
     3901 atttctgttc gaaaacacct ccgcgaggag gccggtgccg agatgacaaa cgtgcagtcg
     3961 ataccaagtc cggcgatcca ttccgaggag cgcgcgcacg aggcggacca tccgagttcg
     4021 ctggtgtcgg cgttcggcgc cgatcaaccg ttgcggcttg attgcggcgt cgatctgtcg
     4081 ccgttccaga tcgcctacca gacctatggc cagctcaatg gcgagcgatc caacgcgatt
     4141 ctgatcttcc atgcgctgac cggcgatcag catgtcgcca acgtccaccc ggtcaccggc
     4201 aagcccggct ggtgggaaac catggtcggt cccggccgtc cgctcgatac cgacaggtat
     4261 ttcatcattt gctcgaacgt gatcggcggc tgcatgggct ccaccggccc ggcatcgacc
     4321 aatcccgcca ccggaaaatt gtgggggctg gattttccga tgatcaccat tcccgacatg
     4381 gtgcgcgcgc aggcgatgct gatcgaccgc ctcggcatag aaacgctgct ctgcgtgacc
     4441 gggggctcga tgggcggcat gcaggcgttg caatggaccg cggcctatcc cgagcgggta
     4501 ttctcggcgc tggcggtcgc ctgcagcacg cgccactcgg cgcagaacat cgccttccac
     4561 gagcttggcc ggcaggcggt gatggccgat ccggactggc gcggcgggcg ctatttcgag
     4621 gaggggacgc atccgcatcg cggtctcggc gtggcgcgga tggccgggca tatcacctat
     4681 ctctcggatg ccgctctgca ccgcaagttc gggcggcgga tgcaggaccg cgatttgccg
     4741 acgttctcgt ttgacgcgga tttccaggtc gagagctatc tgcgctatca gggttcatcc
     4801 tttgtcgagc ggttcgacgc caacagctat ctctatctga cacgggcgat ggattatttc
     4861 gacatcgccg ccgatcacag cggcgtactg gcgcgagcct ttcgcggcat ccagacccgg
     4921 ttctgcgtgg tgtcgttcac cagcgactgg ctgttcccga catcggagtc gcgcgcgctg
     4981 gtgcatgcgc tcaacgcctc gagtgcgcgg gtatctttcg cggagatcga gaccgaccgc
     5041 ggccacgatg cgttcctgct cgacgtgccg gaattcttcg acatctctcg cgcattcctg
     5101 caatccgccg gcacagcgcg tggcctggcc aaaggcgggc ggccgacatg agcctgcagc
     5161 aacaggtttt gccgctcaac ggcatcggct cgcgggacac cggacaatac cgggga
//
LOCUS       DATNOI010000046         4834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1918371,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000046 DATNOI010000000
VERSION     DATNOI010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918371"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VN968_01585"
     CDS             complement(<1..1017)
                     /locus_tag="VN968_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL27974.1"
                     /translation="MSPALSRRTVLQLAGAAALPLSSGLFDAAQAAPSGTLTIAYNVN
                     LPSFDPTVGLSAVNPTIQSIYQSIFDTYVGQAPDLSFKPGLLTKWGWNADRTKITLEL
                     RKGATWHDGSPVTPEDVVWSLQRAADTKSGNPIQFVWSKVGNFKIDGNTITGDVVEFE
                     PTLFKWMAFLTAFVLPKAYYEKVGAEGFEKAPVGSGPYKVDAFERNAFLRLKAHPGYW
                     GAKPAFDTVVFKFVTDPTSRVAEIESGGSDVTFEIPYEEFDRLKAKSGLVGQTKPVSD
                     IAMIFITDIDPMLDRNVRLAANHAIDKKAIVDRLLKGYGVPIATLEAPGYAAFDPTIK
                     VTYDP"
     gene            complement(1077..2318)
                     /locus_tag="VN968_01590"
     CDS             complement(1077..2318)
                     /locus_tag="VN968_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXL27975.1"
                     /translation="MAGQWIDIAAADGGTFKGYLTVPASGSGPGILLLQEIFGVNSSM
                     REVADYYAEEGYVVLAPDLFWRLEPGIELGYSEADFNKAFGYYQRFDANQSIKDAADA
                     LKVLRARSECVGKVGALGFCLGGKLAYLAAARTDVDCAVCYYGVGIEADLGEAAQIKG
                     PIVFHFAELDRFAPAEAREQVKAAFASRPDVELYLYPGCDHAFAAPERTSFNKPATLM
                     AHSRSIALFRKVLGPHYDLSALWDRHTELEFAVRSAEATMATMVAEPYVNHIPTMTGG
                     VGYRDLLRFYANHFIPKTPQDTKLIPISRTIGADRVVDEMLFCFTHDIEIDWMLPGVA
                     PTGKYVEIPLVAIVRFRGDKLYNEHIYWDQASVLVQIGLLDPAVLPVAGVATAKKLVD
                     ETLPSNTLMARWAESAPQAAG"
     gene            2474..3064
                     /locus_tag="VN968_01595"
     CDS             2474..3064
                     /locus_tag="VN968_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HXL27976.1"
                     /translation="MKKTSNAALKKTGGTAKPKSQPAAVERPGSWHLARTETERRLSD
                     FEFGLERLAQAYYRWKAACLAAVCDVPLTGDDVAVLNVVRMGDEPKRLSEIGQLLNRV
                     DVPNLQYATRKLVRSGLIETEGSSSRKETRYRATATGRSVTEAYAALRAATLPPMLEA
                     LDGWAARSETTSIHLDLISSLYAQAAQVAITRRHQP"
     gene            3293..4540
                     /locus_tag="VN968_01600"
     CDS             3293..4540
                     /locus_tag="VN968_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HXL27977.1"
                     /translation="MTDINANAWVSSNHRPMGFAEFVIVIASIMALNPLAMDMMLPAL
                     PTIASAFHIDVANRPQAVLSTFLIGFGVGQFAMGPLSDRFGRRPVLLGGMAVYCVASL
                     LAIAAQSFETLLLARALQGLGTSATRVIATSIVRDCYAGRRMASVMSLAMMVFITVPV
                     VAPSFGQAVMLLTQWRGIFIVLMLYGVLALVWSALRMPETLPVSERKSLAVRDVLGAF
                     RQTVTNRQTLGYALAAGGVQGSLFAFVFSSQQIFTEVFKLGHYFPLAFAAIAIGVAVA
                     GFLNSRFVGRLGMRVISHGALVGFVVVAGLMLVAAKTQMLPLPLFMALAALMMFAFGL
                     MFANFTALAMEPQGHIAGTASSLYGSITTLLGIGIGSTIGQDYDGTLLPFASGFFLCT
                     LAALAVVLVVEKGRLFRPRGMAV"
BASE COUNT          877 a         1507 c         1531 g          919 t
ORIGIN      
        1 aggatcgtaa gtcaccttga tcgtgggatc gaaggcggca tagcccggcg cttccagcgt
       61 cgcgatcggc acgccatacc ccttaagcag ccgatcgacg atcgccttct tgtcgatcgc
      121 gtgattggcg gcgaggcgga cattgcggtc gagcatcgga tcgatgtcgg tgatgaagat
      181 cattgcgatg tcggacaccg gcttggtctg tccgaccaac cccgacttcg ccttcaggcg
      241 gtcgaattcc tcgtagggaa tttcgaacgt gacgtccgac ccgccggact cgatttcggc
      301 gacgcggctg gtgggatcgg tgacgaattt gaataccacg gtatcgaacg cgggcttggc
      361 gccccaatag ccggggtgag ccttgagccg caggaacgcg ttgcgctcga aggcgtcgac
      421 cttgtaagga ccggagccga ccggcgcctt ctcgaaaccc tccgcaccga ccttctcata
      481 ataggccttc ggcaggacaa aggcggtgag aaaggccatc cacttgaaca aggtcggctc
      541 gaattccacc acgtcgccgg tgatggtatt gccgtcgatc ttgaaattgc cgaccttgga
      601 ccagacgaac tggatcggat tgccgctttt ggtgtcggcg gcgcgctgca gcgaccagac
      661 cacgtcttcg ggcgtcaccg gcgagccgtc gtgccaggtc gcccccttgc gcagctccag
      721 tgtgatcttg gtgcggtcgg cgttccagcc ccatttggtc agcaggccgg gcttgaagga
      781 cagatcaggt gcctgaccga cataggtatc gaagatcgac tgataaatcg actggatggt
      841 cggattgacc gcggacaatc cgacggtcgg atcgaacgac ggcaaattga cattataggc
      901 gatggtcagc gttcccgagg gtgctgcttg cgccgcatcg aacagaccgg aggacaacgg
      961 caacgcggcg gcgccggcca gctgcagcac ggtacgacga gaaagcgctg gggacatgac
     1021 cattactcca tatgcgagaa cggcgcccgg gagcgggcgc cgttgacaga ttgcgattat
     1081 ccggcggcct gcggcgcgct ctcggcccag cgcgccatca gcgtgttcga cggcagcgtc
     1141 tcgtcgacga gtttcttcgc ggtcgccacg ccggcaacgg gaagcacggc cggatcgagc
     1201 agcccgatct gcaccagcac cgaagcctga tcccagtaga tgtgttcgtt gtagagcttg
     1261 tcgccgcgga agcggacgat cgcgaccagc ggaatttcca catatttgcc ggtcggcgcc
     1321 actccgggca gcatccagtc gatctcgata tcgtgggtga agcagaacag catctcgtcg
     1381 accacgcgat ccgcgccgat ggtgcgcgag atcgggatca gcttggtatc ctgcggcgtc
     1441 ttcggaatga agtggttggc atagaagcgc agaaggtcgc gatagccgac gccgccggtc
     1501 atggtcggaa tgtgattgac gtagggctcc gccaccatcg tcgccatggt cgcttcggcc
     1561 gagcgcaccg cgaattccag ctcggtatga cgatcccaca gcgcggagag atcgtaatgc
     1621 ggaccaagta cctttcggaa cagcgcgatc gagcgcgaat gcgccatcag ggtcgcgggc
     1681 ttgttgaaac tggtgcgctc gggcgccgcg aaggcatggt cgcagccggg atacaaatac
     1741 agttcgacgt cggggcggct ggcgaaggcg gccttgacct gctcgcgcgc ttccgccggc
     1801 gcgaacctgt cgagttcggc gaagtgaaac acgataggac ctttgatctg cgcggcctcg
     1861 ccgaggtcgg cctcgatgcc gacgccataa tagcagaccg cacaatcgac atcggtccgc
     1921 gccgctgcga gataagcgag cttgccgccc aggcagaagc cgagcgcgcc gaccttgccg
     1981 acgcattcgc tgcgcgcgcg cagcaccttc aacgcatcgg cggcgtcctt gatcgattga
     2041 ttggcgtcga agcgctgata atagccaaac gccttgttga aatcggcttc ggaatagccg
     2101 agctcgatgc cgggctccag ccgccagaac agatccggcg cgagcacgac ataaccttcc
     2161 tcggcatagt aatcggcaac ttcgcgcatc gaggaattga cgccgaagat ctcctgcagc
     2221 agcaggatgc cgggacctga tcccgatgcc ggaacggtga gatatccctt gaacgtgccg
     2281 ccatcagcgg cagcgatatc gatccattgt cctgccactg tactgctccc catcgcaaac
     2341 gcgcgcggtt tcgcgctgcc acttcatcgt gctttcttaa ttgaattttt aatgtaagaa
     2401 tcgcgattgt aaatagcata gattgagcaa ggcttgccta cgcgttgagc gcacgcgaca
     2461 tcaggagacc gttttgaaga agacgtcaaa cgccgcgctg aagaaaaccg gcggcactgc
     2521 gaagccgaaa agccaacctg ctgccgtgga acggccgggc tcctggcatc tggcgcgcac
     2581 cgaaaccgaa cgccggctgt ccgatttcga gttcgggctg gagcggctgg cgcaggccta
     2641 ctaccgctgg aaggcggcat gccttgccgc ggtctgcgac gtgccgctca ccggcgacga
     2701 tgtggcggtg ctcaatgtcg tccggatggg cgacgagccg aagcggcttt ccgaaatcgg
     2761 acaattgctc aaccgcgtcg atgtccccaa tcttcaatac gccacgcgca agctggttcg
     2821 ttcgggcctg atcgaaaccg agggcagctc gtcgcgcaag gagacgcgtt accgggccac
     2881 cgcgaccggg cgttccgtta ccgaagccta cgccgcattg cgcgccgcga cgttgccgcc
     2941 gatgctcgag gcgctggacg gttgggcagc aagatccgag acgacaagca tccacctcga
     3001 tttgatctcg agcctctacg cccaggcggc acaggtcgcg atcacccgac ggcatcagcc
     3061 ctgagaatta gccggcgggc cgcggccggt taaggcctga ggatccggct accccgtcaa
     3121 atccacgccc gttctgaacg gtggcaattt tgcgtcgccg agggctcgcg atcggtcacc
     3181 cggatcggtt tttccctgcg actccaacct tttggacggg gcagccattc ccgaaagcgg
     3241 gttgtgcaac gcagcattgt cgatacaaca ggttttctcc aagaggtgct atgtgactga
     3301 catcaatgcc aacgcctggg tttcctccaa ccaccgcccg atgggttttg ccgaattcgt
     3361 gatcgtcatc gcgtcgatca tggccctgaa tccgctggcg atggacatga tgctgccggc
     3421 gctgccgacg atcgcctcgg cgttccacat cgatgtcgcg aaccggccgc aggcggtgct
     3481 gtcgacgttc ctgattggct tcggcgtcgg ccagttcgcg atgggaccgc tgtccgaccg
     3541 gttcggccgc cgtccggtgc tgcttggcgg catggcggtc tattgcgtcg cgagcctgct
     3601 cgccatcgcg gcgcagtcgt tcgagacgtt gctgctggcg cgggcgctgc agggcctcgg
     3661 cacctccgcc acccgcgtga tcgcgacctc tatcgttcgc gactgctacg ccgggcgccg
     3721 catggccagc gtgatgtcgc tggcgatgat ggtcttcatc accgtacccg tcgtcgcgcc
     3781 gtcgttcggt caagcggtga tgctgctgac gcaatggcgc ggcatcttca tcgtgctgat
     3841 gctgtatggc gtgctggcgc tggtctggag cgcgctgcgg atgcccgaaa ccctgccggt
     3901 gtccgaacgg aagtcgctcg cggtacgcga cgtgctcggt gcctttcgcc agaccgtgac
     3961 caaccggcag acgctgggct atgcgctggc ggccggcggc gtgcaaggct cgctgttcgc
     4021 cttcgtgttc tcgtcgcagc aaatcttcac cgaggtcttc aagctcgggc attattttcc
     4081 gctggcgttc gcggccatcg cgatcggcgt cgccgtcgcc ggatttctca attcccgttt
     4141 cgtcggccga ctcggcatgc gggtgatttc gcacggcgcg ctggtcggct ttgtcgtcgt
     4201 cgccggattg atgctggtcg cggcaaagac gcagatgctg ccgctgccgc tgttcatggc
     4261 gctggcggcg ctgatgatgt tcgcgttcgg actgatgttc gccaatttca cggcgctggc
     4321 aatggagccc cagggccata tcgccggcac ggcctcatcg ctttacgggt cgatcaccac
     4381 cttgctcggc atcggcatcg gcagcacgat cggccaggac tacgacggca cgctgctgcc
     4441 gttcgccagc ggatttttcc tctgcacgct ggccgcgctt gcagtcgtgc tggtggtgga
     4501 aaagggccgg ctgttcaggc cgcgcgggat ggcagtctaa gcgggtcact cgtttctggc
     4561 gcgatccgta ggaaaggcgc cccccgccaa ccggccggga acgcttttcg catggcgcgc
     4621 ggggcgccgt gttgaaccga tccgcgaccg caaacgacaa ttcgtcagcg ggaagaccac
     4681 gccatgcgcc atcggacgta ttccagacgc gacctactcg cgacaacggc ggcgctggcg
     4741 ctggcggttt tcgcacaacc gcccgaacca cggttgatca accctaatct agtcaatgcc
     4801 gcccatgcac acaggagaag acgcgtgacc gaac
//
LOCUS       DATNOI010000047         2211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_691429,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000047 DATNOI010000000
VERSION     DATNOI010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691429"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="VN968_01605"
     CDS             <1..169
                     /locus_tag="VN968_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HXL27978.1"
                     /translation="EHREIVDLVYYHEKSVEEVAEIVGIPGNTVKTRLFYARKKLAEL
                     LKAAGVERGWP"
     gene            166..864
                     /locus_tag="VN968_01610"
     CDS             166..864
                     /locus_tag="VN968_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27979.1"
                     /translation="MMAMSKKILEQEPSEIEMLLPWHAAGTLNARDARRVDEALARDP
                     ELAKQYAVIREEYAETIHLNESLGAPSARTMQKLFAAIDGEPARNPSASLNLSARISE
                     FFAKLSPRTLAWSASLGALALLLQAGVIGAILMKNQTASYQTASLSMNEPVTRNLEAG
                     RPPRALVRFAPDARVADITALLDSYQASIVDGAQGGLFQLQFGNKAMTKDEIAGLISK
                     LQSEKTVSLAVPAQ"
     gene            1023..>2211
                     /locus_tag="VN968_01615"
     CDS             1023..>2211
                     /locus_tag="VN968_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXL27980.1"
                     /translation="MRSPNLNIQSRIPTITPTVVPRIDPNIAGRAVTGIARTPNLRTY
                     PACSYAYRNSDGECSNKPVLSTDGGGTGGSGGASGKNKDKNSGPRRIVAQTALNPRTI
                     TGEIVAEIDGALSDAQADELARRHGLVRLQSQNFPLIGATIGLFRITDRRTVETASRQ
                     FATDTSVRSVQPNFRHFLQEQKAALTEGDPAQYANAKLRLPQAHTLAHGANVTVAVID
                     SGVDVKHPELANAIADSFDALGSKEGPHVHGTGIAGAIVSHARLMGSAPAARILAIRA
                     FAVAPKGGESTSFVILKALDYAAAHGAQIVNMSFAGPNDALIERGISASVAKGIVMVA
                     AAGNAGAKSPPLYPAANRNVIAVSATDSQDRLFAASNRGGHIAVSAPGVDIFLPAPDE
                     KYQM"
BASE COUNT          445 a          690 c          696 g          380 t
ORIGIN      
        1 ggaacaccgg gagatcgtcg atcttgtcta ctaccacgag aaatccgtgg aagaggtggc
       61 tgaaatcgtc gggataccgg gaaacactgt gaagacgcgc ttgttttatg cgcgcaagaa
      121 attggccgag ctgctgaagg cggccggcgt agaacgaggc tggccatgat ggcaatgagc
      181 aagaaaatac tggagcaaga gcccagtgaa atagaaatgc tgttgccatg gcatgcggcc
      241 ggtaccttga atgcgcgcga tgcccgccgc gtcgacgaag cgctcgcccg cgatccagag
      301 cttgccaagc aatatgccgt gatccgggag gagtacgccg agaccattca tctcaacgaa
      361 agcctcggtg cgccgtccgc gcgcaccatg cagaagctgt ttgcggcgat cgatggagag
      421 ccggcgcgca atccctcggc ctcgctcaat ctctccgcgc ggatctcgga gtttttcgca
      481 aaactctcgc cgcggacact ggcttggtcg gcgagcttgg gtgcgctggc gctattgttg
      541 caggccgggg tgatcggcgc gatcctgatg aagaaccaga ccgcctccta ccagaccgcg
      601 tcattgagca tgaacgagcc tgtcacccgc aatttggagg cgggcaggcc gccgcgcgcc
      661 ctggtgcggt tcgcgccgga cgcccgcgtc gccgacatca cggcactgct cgacagctat
      721 caggcctcga tcgtcgatgg cgcccagggc ggcctgttcc agctgcaatt cggcaacaag
      781 gcgatgacaa aggatgagat cgccggtctg ataagcaagc tgcaaagcga gaagaccgtc
      841 agtctcgcgg tgcccgcgca gtaaggcgca cggtgcctcc tccatagcgc atagaccgag
      901 gtctcatggt ctacgatcgt gccaattggc gagtgaaggc gcgccgcgca gcgttggcct
      961 tgacggttgc ggctttgcca tttgcgtatc tcggtggctc ggcggttcaa gcgcaaagcg
     1021 ccatgcgctc gcccaacctc aacattcaat cgcggattcc gaccatcacc cccactgtgg
     1081 tgccgcggat cgatcctaac attgccggca gggcggttac aggaatcgcc agaacgccca
     1141 acctccggac atatccggcg tgcagctacg cctaccgcaa cagcgatggc gaatgctcca
     1201 acaagccggt cttgtcgacc gatggcggcg gtaccggcgg cagcggcggg gcgtcgggca
     1261 agaacaagga caagaacagc ggtccgcggc gcattgttgc gcagacagcg ctcaacccgc
     1321 gcacgatcac aggcgaaatc gtcgccgaga tcgacggcgc gctgtccgac gcgcaggcgg
     1381 acgagctggc gcggcgacac ggcctcgtgc gtctgcaatc gcagaatttt ccgcttatag
     1441 gcgcgaccat cggcctgttc cgcatcaccg accgccgcac ggtggaaacg gcgagccgcc
     1501 aattcgccac tgataccagc gttcgctcgg tgcagccgaa tttccgtcat ttcctgcagg
     1561 agcagaaggc agcactaacc gagggcgatc cggcgcaata cgcgaacgca aaacttcggt
     1621 tgccccaggc gcacacgctg gcccatggcg ccaacgtcac ggttgccgtg atcgattccg
     1681 gcgtagacgt caaacatccg gagcttgcga acgcgattgc agactccttc gatgcgcttg
     1741 gcagcaagga aggtccgcac gtccacggca ccggcatcgc cggcgcgatc gtgtcgcacg
     1801 cgcggctgat gggcagtgcc ccggcagcgc ggattttggc gatccgcgct ttcgccgtgg
     1861 cgccgaaggg cggggagagc acgtcgttcg tgatcctgaa ggcactcgat tacgcggcgg
     1921 cgcacggcgc acagatcgtc aatatgagct tcgccggtcc gaacgatgcg ttgatcgaac
     1981 gcggtatctc cgcctcagtc gcgaagggta tcgtgatggt cgccgccgcc ggcaacgctg
     2041 gagcgaaatc gccgccgcta tatcctgccg ccaatcggaa tgtcattgcg gtcagcgcca
     2101 ccgattccca ggacagattg tttgccgcat cgaaccgtgg cggccatatc gcggtgtcag
     2161 ctcctggcgt cgatattttc ctgcccgcgc cggacgaaaa atatcagatg a
//
LOCUS       DATNOI010000048        14782 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2676192,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000048 DATNOI010000000
VERSION     DATNOI010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 14782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14782
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2676192"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VN968_01620"
     CDS             complement(<1..216)
                     /locus_tag="VN968_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL27981.1"
                     /translation="MSAMLLAQQVLNGLLDGVYYLLIALGLSLIFSLGGIVNLAHGAF
                     YAIGAYLTLVLSPYLGFGGAFVAAPVAV"
     gene            complement(452..1708)
                     /locus_tag="VN968_01625"
     CDS             complement(452..1708)
                     /locus_tag="VN968_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL27982.1"
                     /translation="MFHNNQFTRRGLLKTAGGIALASGISAPAILRAQTADVIRIGHL
                     TPRTGFLGPLGEYAVMAIDLAVEEINASGGMMGRKVELLKEDSVNPQTASTKAERMIE
                     RDKVACIIGEISSASCLTIAQVASRNKTLFINTGGNSDALRGKDCQRYMFHVESQNSM
                     YVKTVGRSFLRDGLVKGKKWYSLTADYAFGHDLLKVSKRFMEANGGNFAGDDLVPTDA
                     TDFSAYLLKIREAKPDLVVINLAGNQITNFLKQYAEFGLTFPVGGFGFDTALAWAAGK
                     GNFVGTWPVVWHHLIDTPGSKTFVAAFTKKYNKPPENQAWGDYTATKIVAQTVNEIKS
                     AESPKIIEHFEKGAKFDVLKTRQGYFRDSDHQLMHEMYAVTALPADQIKNQWDIFTSS
                     PAVPGPNEDLEVIAATKEENSCTFPT"
     gene            1886..2584
                     /locus_tag="VN968_01630"
     CDS             1886..2584
                     /locus_tag="VN968_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXL27983.1"
                     /translation="MNLPAALNLVEPLDRQTLGERAYAKLADLLISGRLAPGEKLSLR
                     AAADVLGVSIMPVREAVSRLVADKALEVAPNRAVRVPVMSAAQFRDLTKVRIAIEGHA
                     AAQAALHRDNADLGLIARAEAAMKAESQSPAPDLPRAVELNKTFHFAVYEAAHSPILV
                     EIIRALWLKAGPVINLDLRANPERLAKGDAIRFHADVRKAIAAGDGEAAREGIKADIS
                     SAADFILSRGGLAG"
     gene            2617..3393
                     /locus_tag="VN968_01635"
     CDS             2617..3393
                     /locus_tag="VN968_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL27984.1"
                     /translation="MDLELKGLRVLVTAGANGIGLAIARAFVGEGARVHTCDVDETAL
                     AVLAASDLAITQTPCDVSDRAAVKNLFAETVAKLGGLDVLVNNAGIAGPTSRVEEMNP
                     EDWDRCLEICLTGQFNCTRLAVPLLRRSKNASIVNISSAAGRLGFAMRTPYAAAKWGV
                     IGLTKSLSIELGPDNIRVNAILPGLVAGDRQRRVLEAKAQQRGISYAEMERTAFSYTS
                     IKDYVTPQQLADQILFMCSPRGKTISGQAISICGDTQMLG"
     gene            complement(3507..5390)
                     /locus_tag="VN968_01640"
     CDS             complement(3507..5390)
                     /locus_tag="VN968_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXL27985.1"
                     /translation="MTASSDDTRSASRSDRRKPAELKGSGATASSDDTRSAPRSDRRK
                     PPRASHYMIAPAALGMTRQALVDRLNRLGDVKILRTYAERDTISPPIAVVRLSDENAA
                     ALRRSTAGAFVIEPDRDLRAASFAGTSSPFHAMAAAAMTPRGPGFTVTIQALSESGEP
                     VEHAEVRLLGEQAAAQGLTGKDGRVDLTLHGERPETVAELFVNARSGYWGLWQHRPDL
                     QADAVNSFTLEPLSLPDRRDWGGIAMRLDQLPTECRGAGIKIALIDSGIATSHKQLTR
                     IDHGIDIRGDEERSWSQDFMGHGTPCAGLIGAIPDSAHGIRGYAPDAELHVCRLPPDA
                     RCSDLVAALDYCLQTGIDVACLGFGCERGSVIVEQRIAAAKQQGVGIIAAAGNSAGTV
                     LFPACSPHVMAVGAIGQTASYPEDSPQAFQAAVAAAVAGGLFVPLFSCRGPELDLCAP
                     GVAVVACQSPDGHAVCDGTSLAAAHVTALAVLILAHHSDFKGGFAGRDFQRVERLFQI
                     LKETAQPVGHPWQTGAGLPDAARALGVWSTRRLLAAPLDTGLREMRSAIRQMNHIYLG
                     AGEANAFGPPPNAFEPPRGPANVTCLPLNPTPLAFQADSGAKAGVHELKAAMMMAGLS
                     DGR"
     gene            complement(5407..5865)
                     /locus_tag="VN968_01645"
     CDS             complement(5407..5865)
                     /locus_tag="VN968_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27986.1"
                     /translation="MSASNPSAASASQPQPPNPDIEQLVGLLSSLMPLLMHFQSQTQQ
                     QPYQAGHFVVPDPALDRQAAESLIGDMIAESLRTLSAYLETNAGLYANLQSCIPIVTQ
                     AAHRFAARDYAEAFNLIWLTYRAIETIRAADPRIPPPRAAASDQPSSSIH"
     gene            complement(5972..6772)
                     /locus_tag="VN968_01650"
     CDS             complement(5972..6772)
                     /locus_tag="VN968_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27987.1"
                     /translation="MTYYDTQQQYLNNPQQWAPLHSQTAQPYGAWLGMSSPQATGGYG
                     LGQAAYGQQYGQYGGQPPMGAFGYGAGWGAQPQAQAWGQLQQPQWGGQQQRQLSQQDV
                     SEVVRQLVPVLPQILAQAQQPHAAIGYAAYGQAPRQLSQQDVNEVVRQILPIVPQIVG
                     TLQGQPQLQYAAMQGGQGLGQQGFGQQGFGQQGLGQFGQGYPAQNLGTQFGFGGPQMQ
                     SAFGAPAAWGQHQRQLTQQDVSDITRQLIGVIPQVIGNLQAFNQQRMI"
     gene            complement(7286..8047)
                     /locus_tag="VN968_01655"
     CDS             complement(7286..8047)
                     /locus_tag="VN968_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HXL27988.1"
                     /translation="MTLLLAVCGWIVGGTEGVHQALAGTAPRPNGPAISREAMFRWFG
                     ARLLRPAELPELFSILIDVCSRARLSRLPDLYCLPAPGDMNAYALGGPGAAAIVLTEG
                     LLRRMTRDEIAGILAHEVAHIRNNDTWTMGWAAALHRSIEWTSLAGLALLRAQHGGMA
                     LSRPLVELLSAAPSIAQLLRLALSRTRELDADATALELTGDSQALIAALDKLERHHAG
                     FPALSVAAFEDGPMRLLRSHPATSERVGALLSLAY"
     gene            complement(8555..10177)
                     /locus_tag="VN968_01660"
     CDS             complement(8555..10177)
                     /locus_tag="VN968_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL27989.1"
                     /translation="MPSDPAVRIKDLRIALPPGGERAYAVDGISFDLAPGEIVCVVGE
                     SGSGKSMCAHALMGLLPDSVTTETGEIALEGCNLLALDADRWRDLRGRRIAMVFQEPM
                     TALNPLMRIGEQMMEMFEAHGLLSPRERRQKALGLAREVGLPDPARIVRAYPHQLSGG
                     QRQRAMIAMALALEPAVLVADEPTTALDVTTQAQILKLIRDLQRSRNMAVMFITHDFG
                     VVADIADRVVVLRHGQIVEQGSAQAVLIRPQHAYTKALLAAVPTMTPPPRSPLIDRNK
                     AVDVIGIDKTYVTSGGWFRPDRTVQAASEVSFAILKGETLGLVGESGSGKSSVARLVM
                     RLIEADRGTIRIGEVDLTQLEGKALRDQRRRIQMIFQDPFASLNPRRKVGQIISDGPI
                     AHGTDPKLARQRARDLLGMVGLDGGAMERYPHEFSGGQRQRIGIARALALDPEILVAD
                     EAVSALDVSVQAQVLNLLEDLKTRLGLSMLFITHDLRVAAQICDRIAVMQRGVIVELK
                     TTATLFAAPEHPYTRELLAAVPGQHRPVSLSA"
     gene            complement(10180..11019)
                     /locus_tag="VN968_01665"
     CDS             complement(10180..11019)
                     /locus_tag="VN968_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL27990.1"
                     /translation="MKQFWKQMSRNPSGLIGLIILVIAVFLAVAGPMVFPNSPWRMVQ
                     RPFLPPFTLSSVPLGTDALGRDVFAGMIYGARVSLLVGLVSTLAALAIGVPLGAIAGY
                     FGGRIDDALMRFTEFFQTIPSFALAIVLVAILQPSIYSIVASIAIVSWPPVARLVRGE
                     VLSLRSREYVQAAIVTGQTSRWIIWHEILPNALSPVIVLASLMVATAILLESSLSFLG
                     LGDPNLISWGYMVGAGRTVIRQAWWITVFPGLAILISVLGLNLVGEGLNDALNPRLAR
                     DGR"
     gene            complement(11016..11987)
                     /locus_tag="VN968_01670"
     CDS             complement(11016..11987)
                     /locus_tag="VN968_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL27991.1"
                     /translation="MLSFVAQRIVKGAIVLLAIIVLNFFLIRLAPGDPAVVMAGEAGA
                     GDQVFVTQLREKFGLDRPLPEQLFLYVKGILSFDLGFSFRQQMPVSKLILDRLPATLL
                     LTMTAFTISLVLGILLGTLAARWAGTWADTAITVVALVFYATPLFWIALMAILLFSVA
                     LDWLPSFGYETIGANYTGFAHILDVGAHLIMPAMTIGLFFMATYARMTRASMLEVKRL
                     DFVKTARAKGLRDKVIQRRHVLRNALLPVVTLAGLHSGTLIGGAVLTETVFAWPGIGR
                     LMYEALLQRDYNLLLGVFVVCSAMVLIFNLVTDLVYRMVDPRIDFSP"
     gene            complement(12067..13614)
                     /locus_tag="VN968_01675"
     CDS             complement(12067..13614)
                     /locus_tag="VN968_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL27992.1"
                     /translation="MLKKIVFGLVLTCSAGLALAQEPRMGGVINAVIQPEPPGLMLAM
                     IQNGPTQMVSGNVFEGLLRYSPKLEPLPGLAESWTVGEDAKVYTFKLKPGVTWHDGKP
                     FTSADVLFSLEFLKQFHARARNNLLQVEKVEAPDDLTVVFTLKQPFGPFLGIFEVGSL
                     PMVPKHLYEGTDYKTNPYNNAPIGTGPFMFKEWQKGSFIRLVKNPNYHIKGKPYIDEI
                     YWQVIPDAAARSVAFETGKIDVLPGGSVENFDVPRLSKLKDVCVTGAGWEFFSPLAWM
                     WLNNRAGPTSNKKFRQAVMYAIDRNFAKDVIWNGLGKVATGPSASTIKFYTDDVPKYD
                     FDPAKAKALLKEAGYNGEKVRLMPLPYGETWLRWGEAVKQNLQDVGINIETVATDVPG
                     SNQKIGDWDYDMAFSYLYQYGDPALGVGRNYVSSAIAKGAVFNNVEGYSNPEIDKLFA
                     DGAVATPDSRRKEIYEKAQKILVEDVPVAWMLELQFPTITRCRIKDLVTTAIGVNDGF
                     RDAWIDK"
     gene            13887..14561
                     /locus_tag="VN968_01680"
     CDS             13887..14561
                     /locus_tag="VN968_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HXL27993.1"
                     /translation="MIPWEHIDTARVPGADVELRLMRRGTDFSMMLGQNELMSSRLSG
                     SEAALATLACRRIAEVERPHLLIGGLGMGFTLRAALAVLGAEARIVVAELVPAVIAWA
                     RGPMADIFGDSLDDPRASIRSADVIEAIKSHASAFDAILLDVDNGPEGLIRKANDALY
                     DLEGLKSIRRALRPGGVLAVWSSGPNAPFSKRLRATGFDVNEVAVRATAKRSGARHVI
                     WFATKS"
     gene            complement(14590..>14782)
                     /locus_tag="VN968_01685"
     CDS             complement(14590..>14782)
                     /locus_tag="VN968_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27994.1"
                     /translation="AAKTAMKIAIKISDDLLRDSAIRQIVNLCLQANDVRTARILFRA
                     IQSVSIREDVLNDYPILRQ"
BASE COUNT         2856 a         4562 c         4649 g         2715 t
ORIGIN      
        1 gaccgcgacc ggcgccgcca cgaaggcgcc gccgaagccg aggtagggtg aaagcaccag
       61 cgtgagatag gcgccgatcg catagaacgc gccatgcgcc agattgacga tgccgccgag
      121 cgagaagatc agcgacaggc cgagtgcgat caggagatag tagacgccgt caagcagccc
      181 attgagaact tgctgcgcta acaacatggc ggacatggtt ttggtccgga cggatgaggg
      241 aaggggtctg aaagcgtcat tgggatccaa cgccgcgcga atatgccaat gccgtcattc
      301 cggggcgatg ccaacgggtc cggcctctgg ccggcccgat gacaggctcc gcatcgaacc
      361 cggaatccaa ggattccccg atgtgcaatt gcacatctga agtctggtgc gaacgcacca
      421 tcccggaatg acggcacttc gagtcggtcg atcaggtcgg gaacgtgcag ctgttctctt
      481 ccttggtggc ggcgatcact tccaggtctt cgttgggacc cggcaccgcc gggctggagg
      541 tgaagatgtc ccactgattc ttgatctggt cggcgggaag tgccgtgacc gcatacattt
      601 catgcatcaa ttgatggtcg gagtcgcgga agtagccttg ccgagttttc agaacatcga
      661 acttcgcgcc cttctcgaag tgctcgatga tcttgggcga ttccgccgat ttgatctcgt
      721 tgacggtctg cgccacgatc ttggtggcgg tgtaatcgcc ccaggcctgg ttctccggcg
      781 gcttgttgta tttcttggtg aaggccgcga cgaaggtctt ggagccgggc gtgtctatca
      841 ggtgatgcca gaccaccggc caggtgccga caaaattgcc cttgccggcc gcccaggcca
      901 gcgccgtgtc gaagccgaac ccgccgaccg ggaaggtcag gccgaactcg gcatattgct
      961 tgaggaagtt ggtgatctgg ttgccggcga gattgatcac gaccagatcg ggcttggcct
     1021 cgcggatttt caacagatag gccgagaaat cggtggcgtc ggtcggcacc agatcgtcgc
     1081 cggcgaaatt gccgccattg gcttccatga agcgttttga aaccttcagg aggtcatggc
     1141 cgaacgcgta atcggccgtc agcgaatacc acttcttgcc cttgaccagg ccgtcgcgca
     1201 ggaaggagcg gccgacggtt ttgacataca tcgagttctg cgactcgaca tggaacatgt
     1261 agcgctggca atccttgccg cgcagcgcat cggagttgcc gccggtgttg atgaacagcg
     1321 tcttgttgcg cgaggcgacc tgcgcgatgg tcaggcacga ggcggaggaa atctcgccga
     1381 tgatgcaggc caccttgtcg cgctcgatca tgcgctcggc cttggtcgag gcggtctgcg
     1441 gattgaccga gtcttccttg agcagttcga ccttgcggcc catcatgccg cccgacgcgt
     1501 tgatctcttc caccgcaagg tcgatcgcca tcacggcgta ttcgccgagc ggcccgagga
     1561 aaccggtgcg cggcgtcagg tggccgatcc ggatgacatc agccgtctgc gcgcgcaaga
     1621 tcgccggcgc ggaaatcccc gatgccaatg caatgccgcc ggcggtcttc aacaggccgc
     1681 ggcgcgtgaa ttgattgttg tgaaacatca gccttggtct ccctgatacg gccgcgtgga
     1741 ctgcggctgt ctgttctccg gcagatggcg tcgccccctg aggcgcgctt tgtcttgccg
     1801 tgatctgtga tcacagttag agttgcaaaa gaccgggccg ttgtcgagtc ctctctcgaa
     1861 cgtggcccgc actgggagaa acgcgttgaa tttgcccgcc gcgctcaatc tggtggagcc
     1921 gctggaccgt cagacgctgg gtgagcgcgc ctatgcgaaa ctggccgatc tcctgatttc
     1981 cggccggctg gcgccgggcg aaaaactgtc gctgcgggcg gcggcggatg tgctcggcgt
     2041 ctccatcatg ccggtacgcg aggcggtgtc gcggctggtc gccgacaagg cgctggaggt
     2101 agcgccaaac cgcgcggtgc gcgtacccgt gatgtcggcg gcgcaatttc gcgatctcac
     2161 caaggtgcgc atcgccatcg aaggccatgc cgcggcgcaa gcagcgctgc atcgggacaa
     2221 cgccgacctc gggttgatcg cgcgcgccga agcggcgatg aaggcggaaa gccaatcccc
     2281 ggcgcccgat ctgccgcgcg cggtcgaact caacaagacc tttcattttg cggtttatga
     2341 agcggcgcac tcgccgatcc tggtcgaaat catccgcgcg ctctggctca aagccgggcc
     2401 ggtgatcaat ctcgatctgc gcgccaatcc cgaacggctg gcgaagggcg acgcgatccg
     2461 gtttcacgcc gatgtgcgca aggccatcgc cgccggcgac ggcgaagcgg cgcgtgaggg
     2521 aataaaagcc gacatcagca gtgccgcgga tttcattctg tcccgcggcg gtctggccgg
     2581 ctgacacttt cttgaaacga cattctcgga gcggtcatgg atctcgaact caagggttta
     2641 cgcgtgctgg tcaccgccgg tgccaacggc atcgggcttg cgattgcgcg tgcttttgtc
     2701 ggcgaaggcg ccagggttca cacctgtgat gtcgatgaaa cggcgttggc cgtgctcgcg
     2761 gcaagcgatc tcgcgatcac gcagacacca tgcgatgtct ccgatcgcgc cgcggtgaag
     2821 aatctgttcg cggagacggt cgccaaactt ggcggcctcg acgtgctcgt caacaatgcc
     2881 ggcatcgccg gaccgacctc cagggtcgag gagatgaatc cggaagactg ggaccgctgt
     2941 ctggagattt gcctgaccgg ccaattcaac tgcacccggc ttgcggtgcc gctgttgcgc
     3001 aggagcaaga acgcctcgat cgtcaatatc tcgtcggccg cgggccggct cggctttgcg
     3061 atgcgcaccc cctatgccgc ggcgaaatgg ggcgtgatcg gactgacaaa atcgctgtcg
     3121 atcgaactcg ggccggacaa tatccgcgtc aatgcgatcc tgcccggcct cgtcgccggc
     3181 gaccgccagc gccgcgtgct ggaagccaag gcgcagcagc gcgggatttc ctacgccgag
     3241 atggagcgca ccgcgttttc ctacacctcg atcaaggact atgtgacgcc gcaacaactc
     3301 gccgaccaga tcctgttcat gtgcagcccg cgcggcaaaa cgatttcggg gcaggcgatc
     3361 tcgatctgcg gcgatacgca gatgctgggc taggcgacaa ggaatgttca ggcgcttgct
     3421 cagccgagtc cgcaccatga gcacggcgcc tcccgaccag gcccgagaat ccgttcgcca
     3481 cgaaaagcgg gtcaagcgta aaacgactag cggccgtcgg acagacccgc catcatcatc
     3541 gctgccttga gctcgtgcac gcccgctttc gcgccgctgt cagcctggaa cgccagtgga
     3601 gttgggttca gcggcaagca agtgacgttg gctggaccac gcggcggttc gaatgcattc
     3661 ggcggcggtc cgaatgcatt cgcctcgcca gcgccaaggt aaatgtggtt catctgccgg
     3721 atagcgctgc gcatctctcg caacccggtg tcgagcggcg ccgccaggag ccgccgcgta
     3781 gaccacacgc caagcgcgcg tgccgcatcg ggcagcccgg cgcccgtctg ccagggatgg
     3841 ccaaccggct gcgccgtctc cttgagaatc tggaacagcc gctcgacgcg ctgaaaatcc
     3901 cgcccggcga agccgccctt gaaatcgctg tggtgggcga ggatcaagac cgcgagtgcc
     3961 gtcacatggg ccgcggccag cgaggtgccg tcgcaaaccg cgtggccatc cggcgactgg
     4021 cacgcaacga cagcgacgcc gggcgcgcac aggtcgagtt cgggtccgcg acaagagaac
     4081 agaggcacaa acagcccgcc tgcgaccgct gcggccaccg cagcctgaaa cgcctgtggg
     4141 ctgtcttccg ggtagcttgc ggtttgtccg atcgctccaa ccgccatgac atgcggagag
     4201 caggcgggaa acagcactgt tccggcagaa ttgccggcgg cggcgataat gccgacgcct
     4261 tgttgctttg ccgcggcgat acgctgctcg acgatgaccg agccgcgctc gcaaccgaac
     4321 cccaggcacg caacgtcgat accggtctgc aggcaatagt cgagcgccgc tacgaggtcg
     4381 ctgcaacgcg cgtcgggagg aagtctgcaa acatgcagtt cagcgtccgg tgcgtagccg
     4441 cggatgccgt gggcgctatc cggaatggcg ccgatgagcc ccgcgcacgg cgtgccgtgg
     4501 cccatgaaat cctgcgacca ggatcgttct tcgtcgcccc tgatatcgat gccgtgatca
     4561 atcctcgtca gttgtttgtg gctggtagcg ataccgctgt cgatgagagc gatcttgatt
     4621 ccagccccgc gacattcagt cgggagttga tccaatcgca ttgctatgcc gccccaatcc
     4681 cgccgatcgg gcagcgacaa cggttccagg gtaaagctgt tcaccgcgtc ggcctggaga
     4741 tccggtcgat gctgccacag gccccaatag ccggagcgcg cattgacaaa cagctcggcg
     4801 accgtttccg gccgctcgcc gtggagagtt agatcgactc tgccatcctt accggtcagc
     4861 ccttgagctg ccgcttgttc cccgagcagt cgcacctcgg cgtgctccac gggctcgccg
     4921 ctttcgctca atgcctggat cgtcaccgta aaacctgggc cgcgcggggt cattgcggcc
     4981 gcagccatcg cgtggaacgg cgacgacgtg cccgcgaacg atgcagctcg caggtcccgg
     5041 tccggctcga tgacgaacgc gccggccgta gaccggcgta acgcggcggc gttttcgtcg
     5101 gacaagcgca ccactgcaat cggcggggag atggtgtcac gctccgcgta ggttcgcagg
     5161 attttgacat cgcccagccg gttgagccga tcgaccagtg cctgccgggt catgcccagt
     5221 gccgccggcg cgatcatgta gtggctggcg cgcgggggct tgcgtcgatc gctgcgcggt
     5281 gcggagcgcg tatcatctga acttgcagtc gcgccgctgc ctttcaattc cgcgggcttg
     5341 cgtcgatcgc tgcgcgaggc ggagcgcgta tcgtctgaac ttgcagtcat ggcgttgcct
     5401 ctcaattcaa tgaattgatg acgatggctg gtcggacgcg gccgcccgcg gtggcggaat
     5461 ccggggatcg gccgcccgga tcgtttcaat cgcgcggtac gtcagccaaa tcaggttgaa
     5521 ggcctcagca taatcccgcg cggcaaacct gtgcgccgcc tgcgtaacga tgggaataca
     5581 actttgcagg ttagcatata gcccggcatt ggtctcaaga taggcggaga gagtgcgcag
     5641 cgattcggcg atcatgtctc cgatcaggct ttccgccgcc tgacgatcga gcgccgggtc
     5701 cggaaccacg aagtgaccgg cctggtatgg ctgctgctgg gtttgcgact ggaagtgcat
     5761 cagaagcggc atcaagctgc taagcaaccc aacaagctgc tcaatgtctg ggtttggcgg
     5821 ctgcggctgc gatgcgctcg cagcagaagg attggaagct gacataggtg ttctccttca
     5881 tcgggacgcg ttcataacga ccggcggcga ccgccgcctt cggcaggcgg cggccgcaga
     5941 ttgccactcg ggctggcagc acaatttgct gtcagatcat ccgctgctgg ttgaaagcct
     6001 gaaggttccc gatgacctga gggatcaccc cgataagctg gcgcgtaatg tctgagacat
     6061 cctgctgtgt cagttgcctc tggtgttgcc cccacgcggc cggggcgccg aacgccgact
     6121 gcatttgcgg tccgccaaat ccgaattgcg tccccaagtt ctgcgcagga tagccctgtc
     6181 cgaattggcc cagaccttgt tggccgaagc cttgctggcc gaagccttgc tgacccaaac
     6241 cttggccgcc ctgcatggcg gcgtattgta gctgcggctg gccctgcagc gttccgacaa
     6301 tctgtggcac gattggcagg atctgccgca ccacttcgtt cacatcctgc tggctcagct
     6361 ggcgcggcgc ttgtccgtaa gcggcatatc cgattgccgc gtgcggctgt tgcgcctgag
     6421 ccaggatctg aggcaggacc ggcaccaatt ggcgcaccac ttcgctcaca tcctgttgcg
     6481 aaagctggcg ctgttgctga ccgccccact gaggttgctg cagttggccc cacgcctgag
     6541 cctgaggttg tgcaccccat ccggcgccgt atccaaaagc acccatgggc ggctgaccgc
     6601 catattggcc gtattgctgg ccgtaggctg cttgccccag tccataaccg ccggtcgcct
     6661 gtggcgacga catgccaagc caggcgccat agggctgtgc cgtttgcgaa tgcaacggtg
     6721 cccattgctg cggattgttg agatattgtt gctgggtatc gtagtaggtc atcgctgatc
     6781 tccttgcgct ggttgtggcc ctcagcccat aacgggacaa agctcgtctg ccttcgcatc
     6841 cgccgttatc cggatgcggg caaaccagcg tcacatttcg tgtgacgcga tcactcatac
     6901 tcaatcggga ctctcggcca atctcgctgc cgccgtcctc aggcgagcga tgaacggcga
     6961 cgatgaatgg caaaaagtac aaatgcaacg catgctcgtg ccggcgtttt caaccgggtt
     7021 gcgagctaat cttggttctg caaggtgttc tgcgttagct cccgaagact cgtttttcgc
     7081 tgtcgggaat actcgacgtt tgtcgtcgaa tctcaaaaga tgcagctaca atgcagcgta
     7141 tgagcggcgc ttttgtgaag gttgggcggt ctttttgtgg aaactctgtg cgcggttcca
     7201 aatcgccgcg gtcttccccg cgtaaccggc gaaacgcatt tacgtaattc gatttgaatg
     7261 tcagctacgt aattcaactt gaatgtcagt aggcaaggct cagcagcgcg ccgacccgct
     7321 ccgaagttgc gggatggctg cgaaggagac gcattggacc atcctcgaac gcagcgacgg
     7381 acagggccgg aaaaccggcg tggtgacgtt ccagcttgtc gagcgcggcg atcagcgctt
     7441 gtgagtctcc ggtcaattcg agcgccgtcg catccgcgtc caactcgcgg gtgcgggaca
     7501 gtgccaaacg aagcaattga gcgatcgaag gagcggcgct cagcagttcc accagcggtc
     7561 gactaagtgc catgccgcca tgttgcgctc gcaacaacgc gagaccggcc aacgacgtcc
     7621 attcaatcga acgatgcagt gcggccgccc agcccatcgt ccaggtatcg ttgttgcgga
     7681 tgtgggcgac ctcatgggcc aggatcccgg cgatctcatc gcgcgtcatg cggcgcagca
     7741 ggccctcggt caatacgatc gcggcggctc cagggccacc cagcgcgtac gcgttcatat
     7801 cgcccggagc tgggaggcaa tagagatccg gcaatcgaga taagcgggct cggctgcaaa
     7861 cgtctatcag tatactaaac agctccggca gttcggcagg acgcagcagg cgggcgccga
     7921 accagcgaaa catggcctcg cgcgagatcg ccggaccgtt cggtcgcggc gcggttccag
     7981 ccagcgcttg gtggaccccc tcggttccac caacgatcca gccgcatacc gcaagcaaca
     8041 gcgtcatccc agcgattgcc aatgcagacg atcggtggtt cgccgcttga tgagcgggcc
     8101 gccgaaaacg cgacggattc cgatcctggg aatgcacgtc gatgctgcag cgcagaacag
     8161 cgccaggcag attcgtcggc aaatgcaagt ctacgatgac ggacgcgcca gaacgcctaa
     8221 gctgcagcat ttccgggcgc ggtgcagaac gcaccaacgc cagtgaaatg cagggcgcca
     8281 ggccggtaac aacgtattgt tcagtccgac ccccgcgcca cgtcccgcca tcagcgctga
     8341 cgttgaacac cgttagcccc ccaaatcgct accacagtaa gataggctcc gcatgcaaca
     8401 gtgtcatgcc aatttcgtgc catagacaca ggaaagtcac agatagacac aggaaagtta
     8461 catgcagcag cggaataccc gaagccggtt gcgtgggcgt tcgccgcaaa tcgtggcgca
     8521 agatgccggc atagacggcg agataaaaag gcccttacgc cgacagggaa accggcctgt
     8581 gctggccagg aaccgccgcg agaagttccc ttgtgtaggg atgttcgggg gccgcgaaca
     8641 acgtcgccgt ggttttgagt tcgacgatca cgccgcgctg catcacggcg atgcggtcgc
     8701 aaatctgcgc ggcaacgcgc agatcgtggg taatgaaaag catcgacagc ccaagccggg
     8761 tcttcaagtc ttccagcagg ttcagcacct gcgcctgcac cgagacgtcg agtgcggaga
     8821 ccgcctcatc cgcgacaagg atttccggat cgagcgccag tgcgcgggcg atgccaatgc
     8881 gttggcgttg tccgccggag aactcgtggg ggtagcgttc catcgctccg ccatcgagcc
     8941 caaccatgcc caggagatcc cgggcgcgct gtcgcgccag ttttgggtcg gtaccatgcg
     9001 cgatcggtcc gtcgctgatg atctgtccga ccttgcggcg tggattgagc gaggcaaacg
     9061 gatcctgaaa gatcatctgg atgcggcggc gctggtcgcg cagcgccttg ccctcgagct
     9121 gcgtcagatc gacctcgccg atccggatgg tgccgcgatc ggcctcgatc agccgcatca
     9181 ccagacgcgc caccgatgac ttgccggagc cggactcgcc gaccaggccg agcgtctcgc
     9241 ctttcagaat cgcgaagctg acttcgctcg cagcctgcac cgtgcgatcc ggccgaaacc
     9301 agccgcccga ggtgacatag gtcttgtcga tcccgatcac gtcgaccgcc ttgttgcgat
     9361 cgatcagcgg gcttcgcggc ggcggcgtca tcgtaggcac cgcggcgagc agcgccttgg
     9421 tgtaggcgtg ctgcgggcgg atgagcaccg cctgtgcgct gccttgctcg acgatttgcc
     9481 catgccgcag cacgacgacg cgatcggcga tatcggccac cacgccgaaa tcgtgggtga
     9541 tgaacatgac cgccatattg cggctgcgct gcaggtcgcg gatcagcttg agaatctgcg
     9601 cctgcgtggt gacgtcgagc gcggtggtcg gctcgtccgc caccagcacc gcgggctcca
     9661 gcgccagcgc catcgcaatc atcgcccgct gacgctggcc gccggacagt tgatgcggat
     9721 aggcgcgcac gatcctcgca gggtcgggaa ggccgacttc ccgtgcgagt cccagcgcct
     9781 tttgccggcg ttcgcgcggc gacagcaggc cgtgcgcctc gaacatttcc atcatctgct
     9841 cgccaatccg catcaaggga ttgagcgccg tcatcggctc ttgaaacacc atggcaatgc
     9901 ggcgtccgcg caggtcgcgc catctgtccg cgtcgagcgc cagcagattg cagccttcca
     9961 acgcgatctc gccagtttca gtggtgacac tgtccggcag caatcccatc agcgcgtgcg
    10021 cgcacatcga cttgccggag ccggattcac ccaccacgca gacgatttcg ccaggcgcaa
    10081 ggtcgaacga aatgccgtcg acggcgtaag cgcgctcgcc gccgggcggc agcgcgattc
    10141 tcaaatcctt gatgcggacg gcgggatcgg agggcatggt cagcggccat cccttgcaag
    10201 ccgcggattg agggcgtcgt tgaggccctc gccaaccaga tttaatccca gcacggagat
    10261 caggatggcg aggcccggaa acaccgtgat ccaccaggcc tgccggatca cggtgcggcc
    10321 ggcgccgacc atgtagcccc acgaaatcag attgggatcg ccgaggccga ggaacgacag
    10381 cgaggattcc agcaggattg cggtcgcgac catcagcgat gccagcacga tgaccggcga
    10441 gagcgcgttg ggcaggattt cgtgccagat gatccatcgg ctggtctggc cggtgacgat
    10501 ggcggcctgg acatattcgc ggctgcgcaa agacagcacc tcgccgcgca ccagccgggc
    10561 caccggcggc cagctgacga tggcgatcga ggcgacgatc gaatagatcg acggctgcag
    10621 gatcgccacc agcacgatcg cgagggcgaa gctcggaatg gtctggaaga actcggtgaa
    10681 acgcatcagc gcgtcgtcga tgcggccgcc gaaatagccg gcgatggcgc cgagcggaac
    10741 gccgattgcc agcgccgcca gtgtcgagac gaggccaacc agcagcgaga cgcgcgcacc
    10801 gtaaatcatg ccggcaaaga catcgcgtcc gagcgcgtcg gtgccgagcg gaacactcga
    10861 tagtgtaaaa ggcggcagga acggccgttg caccatgcgc cacggcgaat tcgggaaaac
    10921 catcggcccg gctacggcga ggaagacggc gatcacgagg atgatcaggc cgatcagccc
    10981 gctgggattg cgcgacatct gcttccagaa ctgcttcatg gggaaaagtc gatgcgcgga
    11041 tcgaccatgc ggtagaccag atcggtgacg agattgaaga tcagcaccat tgccgaacac
    11101 accacgaaaa caccgagcag cagattgtag tcgcgctgca gcagcgcctc atacatcagc
    11161 cggccgatcc cgggccaggc gaataccgtt tcggtcagca ccgcgccgcc gatcagggtg
    11221 ccggaatgca ggccggccag ggtcaccacc ggcaacaacg cgttgcgcag cacatggcgg
    11281 cgctggatga ctttgtcgcg gagccccttg gcgcgggcgg tcttgacgaa atcgagccgc
    11341 ttcacctcca gcatcgaggc gcgcgtcatc cgcgcatagg tcgccatgaa gaacaggccg
    11401 atcgtcatcg caggcatgat cagatgcgcg ccgacgtcca ggatgtgggc aaagcccgtg
    11461 taattggcgc cgatggtctc gtagccgaaa ctcggcagcc aatccagcgc caccgagaac
    11521 agcaggatcg ccatcagcgc gatccagaac agcggcgtgg catagaacac cagcgccacc
    11581 accgtgatcg cggtatccgc ccaggttccg gcccagcgtg ccgccaatgt accgaggaga
    11641 atgccgagca cgagcgagat cgtgaacgcg gtcatcgtca gcaacaaggt cgccggcagc
    11701 cgatccagga tcagtttgga gaccggcatc tgctggcgaa acgaaaagcc gagatcgaag
    11761 ctgaggatgc ccttgacgta gaggaacaat tgctccggca gcggccggtc gagaccgaat
    11821 ttctcgcgca attgcgtcac gaaaacctgg tcgccggcgc cggcctcgcc ggccatgacg
    11881 acagccggat cgccgggagc aagccggatc aggaagaagt tcaagacgat gatcgcaagc
    11941 agaacgatcg cgcccttgac gatgcgctgg gcaacgaatg acagcatcaa agactcattt
    12001 tacctgcgct gcaatccgcc gaccccgatg cccgacaagc gccggtatgg cgtgccgccg
    12061 cagcgttcac ttgtcaatcc aggcgtcgcg gaagccgtca ttgacgccga tggctgtcgt
    12121 aacaaggtcc ttgatcctgc aacgggtgat ggtcggaaat tgcagttcga gcatccatgc
    12181 caccggcacg tcctccacca ggattttctg cgccttttcg tagatttcct tgcgcctgga
    12241 atccggtgtc gcgaccgcac cgtcggcaaa cagtttgtcg atctccgggt tggaatagcc
    12301 ctcgacattg ttgaacaccg cacccttggc gatcgcgctg gagacatagt tgcggccgac
    12361 gccgagtgct ggatctccgt actggtaaag ataggagaag gccatgtcgt agtcccagtc
    12421 gccgatcttc tggttcgaac cgggcacgtc ggtggcgacg gtctcgatat tgatgccgac
    12481 gtcctgcagg ttctgcttga cggcttcgcc ccagcgcagc caggtctcgc cgtaaggcag
    12541 cggcatcagg cgcaccttct cgccgttgta gccggcttcc ttcagcagcg ccttggcctt
    12601 ggccgggtcg aagtcgtatt tcggaacgtc atccgtgtaa aacttgatgg tggatgccga
    12661 cgggccggtg gcaaccttgc cgagcccatt ccagatcacg tccttggcga agttgcggtc
    12721 gatcgcgtac atcacggcct gacggaattt cttgttcgac gtcgggccgg cgcggttatt
    12781 gagccacatc caggccagcg ggctgaaaaa ttcccagccg gcgccggtga cgcagacatc
    12841 cttcaatttg ctgagccgcg ggacatcgaa attttccacc gagccgccgg gcagcacgtc
    12901 gatcttgccg gtctcgaatg cgaccgagcg agccgcggca tccggaatca cctgccagta
    12961 aatttcatcg atgtaaggct tgcccttgat gtggtagttg ggattcttga cgagacgaat
    13021 gaacgagccc ttctgccatt ccttgaacat gaacgggccg gtaccgatcg gcgcgttatt
    13081 gtaaggattg gtcttgtagt cggtgccttc gtacaaatgc ttcgggacca tcggcagtga
    13141 gccgacctcg aagatgccga ggaatggccc aaacggctgc ttcagcgtga atacaaccgt
    13201 caaatcatcc ggcgcctcga ccttctcgac ctgcagcaga ttgttgcgcg cgcgggcatg
    13261 aaactgcttg agaaattcga gcgagaacag aacgtcggct gatgtgaatg gcttgccgtc
    13321 atgccaggtc acgcccggct tcagcttgaa ggtgtagacc ttggcgtcct cgccgaccgt
    13381 ccagctttcg gcgaggccgg gcagcggctc cagtttcgga ctgtaccgca gcagaccttc
    13441 aaagacattg cctgatacca tctgggtcgg tccattctgg atcatcgcca acatcaggcc
    13501 gggcggctcg ggctggatga cggcgttgat gacacctccc atcctcggct cctgtgcgag
    13561 cgcaagacct gcgctgcagg tcaacacgag gccgaatacg attttcttga gcatgactta
    13621 ccttttgacg gctcgatccg cggacggcaa gccctgttga agcacgagaa tgcaggcttt
    13681 tgggctggtt gaaatccctg tcgaagggag gctcacatgg cgtcaatcca ggccgcaaga
    13741 tgaaactgtc gacaaacggc gactaaaatt tggacaactc acggctcaac gcctatgcct
    13801 gcgataccgg caaaaacaga gcgcgacgtc gcggtcgcct gtgccaggcc tcgcctggta
    13861 gcgtcaaaac agcgtagaag cttcgcatga ttccgtggga acatatcgat accgcgcgcg
    13921 tgcctggcgc agacgtcgaa cttcgtctga tgcgaagggg gactgatttc tccatgatgc
    13981 tcggccagaa cgaactgatg agcagtcggc tcagtggatc cgaggcagcg cttgccacgc
    14041 tcgcctgccg caggatcgca gaggtcgagc gcccgcatct gttgatcggt ggattgggca
    14101 tgggattcac cctgcgcgcc gcacttgccg tattgggagc cgaagcgcgg atcgtggtgg
    14161 ccgagcttgt gcccgccgtg atcgcatggg cacggggtcc gatggccgat atcttcggcg
    14221 atagcctaga cgatccccgc gcaagcattc gcagcgccga cgtcattgag gcgataaaat
    14281 cccatgcctc ggcgttcgat gcgatcctgc tcgatgtcga caacggcccg gagggcctga
    14341 tccgtaaagc caatgatgcg ctttacgatc tggaagggct gaaatcgatc cgccgggcct
    14401 tgcgcccggg cggcgtcctc gccgtctggt cgtcgggacc taacgctccg ttctccaagc
    14461 ggcttcgcgc caccggcttc gatgtgaacg aggtggccgt gcgcgccacc gcaaagcgca
    14521 gcggcgcgcg tcatgtgatc tggtttgcga cgaagtcttg aggtcggcgc cgatctgtca
    14581 gcgccgcgct cactgccgca gtatcggata gtcattgagc acgtcttccc tgatcgacac
    14641 agactgaatc gcgcgaaata gtatccgcgc tgtcctcaca tcgttggcct gcaaacacag
    14701 attgacgatc tggcgtattg cggaatcgcg caacaggtca tcggaaatct tgatggcgat
    14761 tttcatggcg gtcttggcgg cg
//
LOCUS       DATNOI010000049        11275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2125194,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000049 DATNOI010000000
VERSION     DATNOI010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11275
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2125194"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            422..985
                     /locus_tag="VN968_01690"
     CDS             422..985
                     /locus_tag="VN968_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27995.1"
                     /translation="MFDHLFGAGQKGGWEQYDKCRKKMFEPLVWASCHQDFVARRPDG
                     SVRGVTLILEIDRVDDVTADEDPIVSAVRRSLSQSLLRSDGVSLMSRFTIPEGDRREA
                     RPTMDALQICHLMHWATEQDLHLWIIVAHHPDHFAPLLAEMHFECMLGCDQTVGGMPV
                     GCFVHDWKSEPWLVWRDRRKEVVVALC"
     gene            complement(1388..1579)
                     /locus_tag="VN968_01695"
     CDS             complement(1388..1579)
                     /locus_tag="VN968_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL27996.1"
                     /translation="MEDEKPVLEQMTDMIAETDHVTKEAAKKAVKKVKKVAKKVMPKK
                     STKKKAVKKTTKKKRRSKH"
     gene            complement(2593..2781)
                     /locus_tag="VN968_01700"
                     /pseudo
     CDS             complement(2593..2781)
                     /locus_tag="VN968_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
     gene            3138..3893
                     /locus_tag="VN968_01705"
     CDS             3138..3893
                     /locus_tag="VN968_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HXL27997.1"
                     /translation="MSMYFEELDFRPTAMGVLSLRRRRHLGSGLDIYEIKLGDAFLMS
                     SQFTVAEIELARLGLAALARADIDVVVGGLGLGYTAQAVLENSAVRWLVVVDALAEVI
                     EWHQQGLLPLGKSLTDDPRCRFVHGDFFAMAHAEDGFDPDAPGRRFDAVLVDIDHSPR
                     NLLHPSHAALYTPEGLARLAGHLQPGGVFALWSNDPPDDEINAALACAFATSASHVVT
                     FDNSQGDHDASNTVYVGVKADLPIQKAINKAAP"
     gene            4054..5307
                     /locus_tag="VN968_01710"
     CDS             4054..5307
                     /locus_tag="VN968_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL27998.1"
                     /translation="MRFKHILGPLCAASISVVLAGHALAQDKIVKIGALYPMSGNSAS
                     VGVHAKAAIETAVDIINNAHPELGDFPLARNAGLAGLGGAKIEVVFADSQGSPATGQN
                     QALRLITQDKVIALNGAFHSGVTLTASAIAEKYGIPFLTSESVAANLTERGFKWFFRV
                     TPIATDFARIYYDFLADMKVAGVKTDNVALVHDNTEYGTSVAGVITSVFKEKGQNIAL
                     DVAYSVNATDVQSQVLQLKEKKPDVVIMITYTSDAILYAKTMQALDYKPPLLLGDDAG
                     YSDPSFLKSVGKISQGVFDRSSWSVGPPGSPTALIAEMYKKKSGDEMDDTTGREMQGF
                     FVLVEAIDRAGSTDPAKIQAALKATDLKPDQLMMGYKGVKFDEKGQNTLASGVIIQLQ
                     DGENYVAVWPKAGAEKTPVLPYKGW"
     gene            5424..6287
                     /locus_tag="VN968_01715"
     CDS             5424..6287
                     /locus_tag="VN968_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL27999.1"
                     /translation="MSSILVQVIVGGLLLGAVYALFSSGLTLVWGMMNIVNFAHGDFV
                     MLGMYFAFMVWTLFGAGPWLGAPLAALALATLGVVVYFGLIRDIMKGPMLAQILGTFG
                     LALLLRYSVFWWFGANFLSLPGNIVGGTFDILGLRIEASRLLAGVVALLVTLGLHLLL
                     TRTSLGSKMLAVAEDSTAAQLMGILPDTMQAIAWAIAAGATGLAGALIATFFYIVPTV
                     GETLGIVAFVTVSLGGFGSVPGALVAGLLIGVIESLSAYLIGAVYKDMVVYSLFLGFL
                     WFRPQGLMGKM"
     gene            6287..7279
                     /locus_tag="VN968_01720"
     CDS             6287..7279
                     /locus_tag="VN968_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL28000.1"
                     /translation="MRRLLWCSVVLALFVAYPLLFSTPFQQRLGALVLLYAIAASAWN
                     IVGGYAGQVSVGHVVFFGCGAYAAMGAYAHFGLSPLVGIPTGIVVSVAIAAAIGVPTL
                     RLSGHYFSMATIAVAELVRLIVTNTDYLGAAVGLSGPTVPRNVFDLSFISALPYYYLF
                     LSLLLVTLGITWWMANSRMGFYLRAIRDSERAARSLGAPASRTKLYAFMLSAGLTSVA
                     GALYAMMFGFVDPESGLGILISVKILIMAALGGAGLLFGPLVGAAILVPLEEISNNLL
                     GGQGAGLTFVLYGAIIVLIARFQPGGVLTLIKRMLAKRRKAPETPVAAGATHAP"
     gene            7269..8036
                     /locus_tag="VN968_01725"
     CDS             7269..8036
                     /locus_tag="VN968_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28001.1"
                     /translation="MLLEARNVTRAFGSFKAVDAASVTLEQGDILGLIGPNGAGKSTF
                     FNCLTGDILSTSGEVLFEGHDITNMPPEARARLGLARTFQVPQTFESMSVLENVMIGA
                     FLRTSHRAEAEVRARAVLERVGMSKLADAPARSLGTPGRKRLEIARALATEPKVLLLD
                     EAMAGLTQREVQLAIDLVRDIHRSGITMVIVEHIMEVIMSLTTRVVVFHQGKEIARGS
                     PREVTGIPAVIEAYLGKRAAKAAAGHTPIELMGGPAT"
     gene            8033..8752
                     /locus_tag="VN968_01730"
     CDS             8033..8752
                     /locus_tag="VN968_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28002.1"
                     /translation="MTAPLLRIEHLEVRYGDLIGVSDVSLEVPEGSVVALLGSNGGGK
                     TTTLNAVAGLIPLHAGSIVFGGEEIGGKTAFSIVRKGLALSPEGWRLFVQQSVENNLL
                     LGTTPLRDRARAAALLERVYEIFPKLAERRNQRAGTMSGGERQMLAVGRALMSDPRLL
                     MLDEPSLGLAPAVVESMYDTFGRLHREGLTILLAEQSIELALEVSDFATVLQVGKSVL
                     SGPAAALAEDPQVQKAYLGTG"
     gene            complement(8770..9480)
                     /locus_tag="VN968_01735"
     CDS             complement(8770..9480)
                     /locus_tag="VN968_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL28003.1"
                     /translation="MQYRMRPQSTEFDFPGVTPSRQKRSRETTAALLQAGADMLRTRS
                     LAELSIEALCTKVGATVGAFYSRFESKEAYFNTLIELAARDCNAMLSRMAQNEQLKDT
                     GLPDLCRLLVSAIIGWMRDHEGVLRAALQHDDTRPDRWSTFKGLARASVARATPPLLR
                     VMGKGRKPAKTRGIAVAFQVVLGTLVNAILNDPGPLSLHDKEMEVRLGNCLLLLLEAE
                     MNPASSKTSTGASGPRKK"
     gene            9617..>11275
                     /locus_tag="VN968_01740"
     CDS             9617..>11275
                     /locus_tag="VN968_01740"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HXL28004.1"
                     /translation="MSAGSDTPFRGTIGKTVTDSRPWWPPTRRPPEGAPNILVVLFDD
                     VGFSDFGCYGSPIKTPVIDRLAAEGLRYSGFHTTAMCSTTRAALLTGRNHHSVGVGCL
                     ANFDSGYPGYRGKIAREAGTLAEMLKAHAYRNYMVGKWHVTPLTESGATGPFDGWPLG
                     RGFDRFYGFLDAETDQFAPELVSDNTHIDPPGTYASGYHLTSDLVDQSIRFIADHIAD
                     RPDLPWLTWLALGACHAPHQAPADFIKGYDEIFAHGWDVEREQRLARQIATGIVPPGT
                     RMPDRNDGVKAWDEHSLDEKRIFTRLQSAFAGLLDHADWHLARLIAFLETAGIRDNTL
                     ILVLSDNGASQEGGPLGFVNAMGPYNFRPEPVPEKMRRLDDIGGPDSHTNFPHGWAMA
                     SNTPLRRYKQNTHGGGIRDPFVLSWPKRVSAKGELRHQFVHASDLVPTLLDLIGIAAP
                     AEIAGCAQMPIEGESFACSIADAAAPSKSSPQYFEMFGHRGLWHQGWKAVAYHPPGTP
                     FENDKWELFHLDRDFSEVDDLALSEPDRLADMIQLWWSEAEKHNV"
BASE COUNT         2011 a         3421 c         3530 g         2313 t
ORIGIN      
        1 tgctgacgca gattttgcgc acccaacgat ggccgaaggg ctgagctcat tgttttcgag
       61 cgtgccacct cgcgtggtgc aacagaccaa atcgatggtg gcgtagtgat cccgatctcg
      121 atgacgcaat caccgccaca ccctgggatc ggtcgcgccc acccgcaggg accaggacga
      181 ttcctgcggc agcagacacc cgatcggcca aaccagcgag cataggaacg tcatccccaa
      241 gccgcgccga cttccgctca cccacatccg cgcgatcaaa tctccatagc gcgcggcaac
      301 cgtcgcaccc aaactcacgc gcgttccttc actggaggct ttcggacgac ggcccggtgc
      361 aagccgcgtc gtcgcgatgg gccggcatcc gaaaccctac acagtaccgg aagtcgcgag
      421 tttgttcgat catctctttg gcgcgggcca gaagggcggc tgggagcaat atgacaagtg
      481 ccggaaaaag atgttcgaac ctctggtctg ggcatcctgc caccaggact tcgtagcccg
      541 ccggccagac ggctcggtcc gtggcgtcac attgattctc gagattgatc gagtggacga
      601 cgtgactgcg gacgaggatc cgatcgtctc cgcggtgcgg cgatcattaa gccaatccct
      661 gcttcgctcg gatggcgttt ccctgatgtc gaggttcacc ataccggaag gagatcgccg
      721 tgaggccagg ccgacgatgg atgctcttca aatttgccat ttgatgcact gggctactga
      781 gcaggatctg catctctgga tcatcgtcgc gcaccatcct gatcattttg caccactgct
      841 tgctgagatg catttcgaat gcatgctcgg ctgcgatcag acggttgggg gaatgccagt
      901 aggctgcttt gtgcacgatt ggaagtcgga gccttggctt gtctggcgcg atcggcgcaa
      961 agaggtcgtt gtagccctat gctagatttg gccatctccg gctggctgga cctagcccaa
     1021 acttccccat gatggactgg accgaccagc attgtcatgc ctcccaccgc ctgatgaggc
     1081 gccgtgcccc tctatgccga gatggtggcg tcggccgcac gtgtgaaact caaccactcc
     1141 gatttgtgca aacccattag atcatgctcg atcatttcgg ttccaggatc agctgtgacc
     1201 aaacgtgcaa gtggggtatc gcgtcggatg cttgtggacc aggactatac ggtgcaaaac
     1261 ttgccgaaga actcggtcgg acgcactgca cagaaaccct cgggcttgat cttcgcattc
     1321 cacagcagat gcctcccaac tgagcatccc gtggagacag catggttgca atgcgacgag
     1381 agccgattta gtgtttcgac ctcctctttt tcttcgtagt ctttttcacg gcctttttct
     1441 tcgtggactt cttcggcata actttcttgg cgaccttttt gaccttcttc acggcctttt
     1501 tggcagcctc tttcgtaaca tgatccgttt cggctatcat gtcggtcatc tgctcaagca
     1561 ctggcttttc gtcttccata gcgccctcct agaatgagga tgtgagggta accgattcgg
     1621 gaatgtgccg cgtgctcatg cggcttgatt tgggcgagga cttttggctg gcgccctaaa
     1681 gcttcggtcg tccctacgtt ggatctcgac cgatcgacgc gtatcgaccc tagcgccttc
     1741 aatagcggct gcgcgggcac tgtcatcgca ttctctggcg taggcttcaa cgagcgccgc
     1801 gtctggtgga gacaacatgc tggcaactcg gcgggcctgt cccgcgcgtg cgcgccacat
     1861 gagcgtttcc gataggcccg ctttgctgat catgagaatc accccttgct caacagcaaa
     1921 atgctgtcgg atcggcggtg aatgttcaat gcttgaaatt atttcggaat cgagtccgtc
     1981 tagttgattc tcgacagact ttcgctcgtc cattgcgtga tcgcacgtcc gcgtcacatg
     2041 aagcctttcc cgtcatgatc gacgccatcg catcaagttt ggggtcaatg atcgttggat
     2101 atgaatgctc atttccggcc cagatggaca agcaattgat ggaggtggcc atagcgcttc
     2161 atgctgctcc agcttctgtg acccgaaacg ctcgcggcat gcggcatggt ttttcccatt
     2221 tcgaacagtc gcgaaacacc gtcgcgaacc gaacctgcga gccgggtcga gcgcgcgcat
     2281 cctgctgggt tattgggaag attcgtcgtt tttcgcggtg ggccaagcgg ctcgggctgc
     2341 accatcaaac ggtccagcgc tgcgtcaagc gggcggtggc cgagggcccg atggccgcgc
     2401 cggacgatcg ccctcgccct ggccgggagc cgacgatcac gctggaagcg aaagcctggt
     2461 tgacatcgct gtcgtgccgg aaggcgaagg aactgggtta tccgcatgaa ttgtggacca
     2521 cgcggctgct caccccccac gctcgcgagc acggtcccgc cgaagggcac ccgtgcctgg
     2581 cgaacacgaa aatggccgtc gcgcgagtag ccgcgccaga ttggtcttac gcaggctcaa
     2641 ggggaggggg cgcaaatcct cgccatcgag cgtcaggatg tcgaaggcat acagttggac
     2701 ttcgttatcg tgcttgcgcg agtgcagggc atcgaaatcg ggaacaccgt caaccccaag
     2761 gatgacagcg tcgccatcaa cgtactaatc tgctcaacac cgagagaagc aaacgtctca
     2821 gaagtgaccg gaaatagccg gatcaaaatg aagggggttt gcaaaaccag gtgaaagatt
     2881 tcaacggcct agcccgaaag ccgttgtgct accgttacac cattccccaa gagtactgaa
     2941 tatatcagag acttgaccgg tattcttaga gttctttcgt cggatcgggc aaaatccagc
     3001 gcagagcgtg gcgccgttct actcgttccc tcccggcctt ggcaagcgcg cggcgacggg
     3061 cttggtgcga ggggcggcgg caaagccgcg gcaattcccg ggcgcggttt gcaccctggt
     3121 caacgggaat ccggcgcatg agcatgtatt tcgaggaact ggattttcgc cccaccgcga
     3181 tgggcgtgct cagcctgcgg cggcggaggc atctcggctc aggcctcgac atctacgaaa
     3241 taaaactcgg cgacgcgttt ttgatgtcga gccagttcac ggtcgccgag atcgaactgg
     3301 cgcggctcgg attggcggcg ctggcgcgtg cggatatcga cgtcgtcgtc gggggcctcg
     3361 ggctcggcta tacggcgcaa gcggtgctgg aaaattcggc agtgcgatgg ctcgtcgtgg
     3421 tcgatgcgct ggccgaggtc atcgaatggc accagcaggg cttgttgccg ctcggcaagt
     3481 cactgacgga tgatccgcgc tgccgttttg tccatggcga tttcttcgcg atggcgcatg
     3541 ccgaggatgg cttcgatccc gatgcgcccg gccgccgctt cgatgcggtg ctggtggata
     3601 tcgaccactc tccacgcaac ctgctgcatc cgagccatgc cgcgctgtac acgccggagg
     3661 ggcttgcccg gcttgccggg cacctgcaac cgggtggtgt gtttgcgctg tggtcgaacg
     3721 atcctccgga tgatgaaatt aacgccgcgc tggcttgtgc ctttgcaaca tcggcttcgc
     3781 atgtcgtgac cttcgacaat tcgcaaggtg atcacgatgc cagcaacacg gtctatgtcg
     3841 gggtcaaggc cgatctgcct atccagaagg caatcaacaa ggcggcgcca tagccgattg
     3901 atttgcatgg gcatgtgcgc ggtcggaaac ttgcccctgt gaagcgggca tgcgatgtag
     3961 gtcaacagat accggaagcg acggcggtga cacatcgcgc atcctcttct cggcgttggg
     4021 cgttgtgtgg gaaacgtttc ggagaccctt tacatgagat tcaagcatat cctcgggccg
     4081 ctttgtgctg cctcgatttc ggttgtcctg gcaggccatg ctttggcgca ggacaagatc
     4141 gtcaagatcg gcgcgctgta cccgatgagc ggcaattccg ccagcgtcgg cgtccatgcc
     4201 aaggccgcga tcgaaaccgc tgtcgatatc atcaacaacg cgcatcctga actcggcgat
     4261 ttcccgttgg ccaggaatgc cggcctcgcg gggctcggcg gcgccaagat cgaggtggtt
     4321 ttcgccgaca gccagggcag cccggccacc ggtcagaacc aggcgctgcg gttgatcacc
     4381 caggataagg tgatcgcgct caacggggcc tttcattccg gtgtcacgct gacggcaagc
     4441 gcgattgccg aaaaatacgg aattccgttc ctgaccagcg agtcggtggc cgccaacctg
     4501 accgagcgcg gcttcaagtg gttcttccgc gtcacaccta tcgcaaccga cttcgccagg
     4561 atctattacg atttcctcgc ggatatgaag gtcgcgggcg tcaagaccga caacgtcgcc
     4621 ctcgtccatg acaacaccga atacggcacc tcggtggcgg gtgtgatcac cagcgtgttc
     4681 aaggaaaagg gccagaacat cgcgctcgac gtcgcctatt ccgtcaacgc caccgacgtg
     4741 caaagccagg tgctgcagtt gaaggagaag aagcccgacg tcgtgatcat gatcacctac
     4801 acgtcggacg cgatcctgta cgccaaaacc atgcaggcgc tcgactacaa gcctcccctg
     4861 ctgctcggcg acgacgccgg ctattccgat ccgtcgttcc tcaagtcggt gggcaagatt
     4921 tcgcaaggcg tcttcgaccg ctcgtcgtgg agcgtcggcc cgccgggctc gccgacggct
     4981 ctgatcgccg agatgtacaa aaagaaaagc ggcgacgaga tggacgatac caccgggcgg
     5041 gagatgcagg gttttttcgt cctggtggaa gcgatcgacc gcgcgggttc gaccgatccc
     5101 gccaagatcc aggcggcgct gaaagccacc gacctgaagc ccgatcagct gatgatgggt
     5161 tacaagggcg tcaagtttga tgaaaagggc cagaatacgc tggcttccgg cgtcatcatt
     5221 cagttgcagg acggggaaaa ctatgtcgcg gtctggccca aggccggcgc cgagaagacg
     5281 ccggtcctgc cctacaaggg ctggtaacat ccgggggcgg gacgtccttt cccttaccgg
     5341 tgcagtcgtg gtgtcagcga tcgcttgaac cggacggtca gtaaacccgg cgcaacgcct
     5401 aaatcgaaaa gaccagctgc tttatgtcct ccattcttgt tcaggtgatt gtcggcggcc
     5461 tgctgctggg tgccgtctac gccctgtttt catcgggcct cacgctggtc tggggcatga
     5521 tgaacatcgt caacttcgcg catggcgatt tcgtcatgct cggcatgtac ttcgccttca
     5581 tggtgtggac gctgttcggc gcgggtccct ggctcggcgc gccgctcgca gcgcttgcgc
     5641 tggcgacact cggggtcgtc gtctatttcg gactgatccg tgacatcatg aaggggccga
     5701 tgctggccca gattctcggc accttcgggc tggcgctgtt gctgcgctat tcggtgttct
     5761 ggtggttcgg cgcgaacttc ctgtcgctgc cgggaaatat tgtcggcggc accttcgata
     5821 tattgggtct gcggatcgag gcgtcgcgcc tgctggccgg cgtggtcgca ctgctggtga
     5881 cgctcgggct gcacctcttg ctgacgcgca cctcgctcgg ctcgaagatg ctggcggttg
     5941 cggaggattc caccgcggcg cagctgatgg gcatcctgcc cgataccatg caggccatcg
     6001 cctgggcgat tgcggcgggc gccaccggcc ttgccggcgc gctgatcgcc acattcttct
     6061 acatcgtgcc gaccgtcggc gagaccctgg gcatcgtcgc cttcgtcacg gtgtcgcttg
     6121 gcggcttcgg cagcgtgccc ggcgcgctgg tggcgggact cctgatcggc gtcatcgagt
     6181 ccctgtcggc gtatctgatc ggcgccgtgt acaaggacat ggtggtgtat tcgctgtttc
     6241 tcggctttct ctggttccgg ccgcaaggct tgatgggcaa gatgtgatgc ggcgcttgct
     6301 gtggtgttcg gtggtgctgg cgcttttcgt cgcctatccg ctgttgttct cgacgccgtt
     6361 tcaacagcgg ctcggtgcgc tggtgctgct gtatgcgatc gcggcatcgg cctggaacat
     6421 cgtcggcggc tatgccggcc aggtgtcggt cggccacgtg gtgttcttcg gctgtggcgc
     6481 ctatgccgcg atgggcgcat atgcgcattt cggattgtcg ccactggtcg gaattcccac
     6541 cggcatcgtc gtcagcgtgg cgatagcggc cgcgatcggg gtgccgacgc tgcggctttc
     6601 aggccattat ttcagcatgg cgaccattgc cgtcgccgaa ctggtcaggc tgatcgtcac
     6661 caacaccgac tatctcggtg ccgccgtcgg cctcagcggc ccgacggtgc cgcgaaacgt
     6721 cttcgatctc agtttcattt cggcgctgcc ttattattat ctgttcctct cgcttctcct
     6781 ggtcacgctc ggcatcacct ggtggatggc gaacagccgg atgggatttt atctccgggc
     6841 gatccgggat tccgagcgcg cggcgcgctc gctcggcgcg cccgcaagcc gcaccaagct
     6901 ttacgccttc atgctgagcg ccgggctgac cagcgtcgcc ggcgcgctct acgcgatgat
     6961 gttcggattc gtcgatccgg aatccgggct cggcatcctg atctccgtca aaatcctgat
     7021 catggccgca ctcggcggcg ccggcctgct gttcggaccg ctggtggggg ctgcgatcct
     7081 ggtgccgttg gaggaaatct ccaataacct gctgggcggc cagggcgcgg gcctgacgtt
     7141 tgttttgtat ggcgccatca tcgtgctgat tgcgcgcttt cagccgggcg gcgttctgac
     7201 attgatcaag cggatgctgg caaagcgacg caaggcgccg gaaacaccgg tcgcggcggg
     7261 agcaacgcat gctccttgaa gcccgcaatg tgacaagggc ctttggcagc ttcaaggccg
     7321 tggacgcggc ctcggtgacg ctggaacagg gcgacattct cggcctgatc ggcccgaacg
     7381 gcgccggcaa gtccaccttc ttcaattgtc tgaccggcga catcctgtcg acatccggcg
     7441 aggtgttgtt cgagggccac gacatcacca acatgccgcc ggaggcgcgg gcgcgcctcg
     7501 ggcttgcgcg cacgttccag gtgccgcaga cattcgagag catgtcggtg ctggagaacg
     7561 tgatgatcgg cgcgtttctt cgcacctccc atcgcgccga agccgaggtc cgggcgcgcg
     7621 ccgtgctgga gcgtgtcggc atgagcaaac tggcggatgc gccggcgcgg tcgctcggca
     7681 cccccggccg caagcggctg gagattgcgc gcgcgcttgc gaccgagccc aaggttctgt
     7741 tgctcgatga agcgatggcg ggcctgaccc agcgcgaggt gcaactcgcc atcgatctcg
     7801 tgcgcgacat ccatcgctcg ggcattacca tggtgatcgt cgagcacatc atggaagtca
     7861 tcatgtcgct gacgacgcgg gtggtggtgt ttcaccaggg caaggaaatc gcgcgcggca
     7921 gcccgcgcga ggtcaccggt atccccgccg tgatcgaagc ctatctgggc aagcgcgccg
     7981 ccaaggccgc cgccggccac acgccgatcg agttgatggg tgggcccgcg acatgaccgc
     8041 gcctttgctt cggatcgagc atctggaagt ccgctatggc gacttgatcg gcgtgtccga
     8101 cgtttcgctg gaagtgcccg aaggcagcgt ggtcgcgctg ctcgggtcca atggcggcgg
     8161 caagaccacg acattgaacg ccgtcgccgg cctgatcccg ctgcatgcgg gatcgatcgt
     8221 tttcggtggc gaggagatcg gcgggaagac ggcgttttcg atcgtgcgca agggtctcgc
     8281 gctgtcgccg gagggctggc ggctgttcgt gcagcaaagc gtcgagaata atctcttgct
     8341 ggggacgacg ccgctgcgcg acagggcgcg cgccgcggca ttgctcgagc gcgtctacga
     8401 gatctttcca aaactggcgg agcgacgcaa ccagcgcgct ggcaccatgt ccggcggcga
     8461 gcggcagatg ctggcggtcg gccgtgcgct gatgagcgat ccaagacttc tgatgctgga
     8521 cgagccatcg cttggtctcg cgccggccgt ggtcgagtcg atgtacgaca ctttcggccg
     8581 gctgcaccgg gaggggctga ccatcctgct ggcggagcaa tccatcgagc tcgcgctgga
     8641 ggtttcggat ttcgcgaccg tgctgcaggt cggcaaaagc gtcttgtccg gcccagcggc
     8701 ggcgttagcc gaggatccgc aggttcagaa ggcctatctc ggcaccggct gaagttggaa
     8761 gccctaagct tatttcttgc gcgggcctga ggcgccagtc gaagttttgg acgatgccgg
     8821 attcatctcg gcttcaagca gcagcaaaag acagttgccg agccgaactt ccatctcctt
     8881 gtcgtgaagg gataacggtc cggggtcgtt gaggatggcg ttgaccagcg ttcccagcac
     8941 cacctgaaag gccacggcga tgccgcgtgt cttcgcgggc ttgcggccct tgcccatgac
     9001 gcggagcagg ggaggcgtgg cccgcgcgac cgatgcccgc gccaaaccct tgaacgtgct
     9061 ccaccggtcc ggccgggtat cgtcgtgctg cagtgccgca cgcaagacgc cttcgtggtc
     9121 ccgcatccag ccgatgatcg cgctgaccag gaggcggcac aggtcgggga ggccggtgtc
     9181 cttcaattgc tcattctgcg ccatccgcga cagcatggca ttgcagtcac gcgccgcgag
     9241 ttcgatcaag gtattgaagt aggcctcctt gctctcgaac cggctgtaga aagcgccgac
     9301 ggttgcgccg acttttgtgc acagcgcttc gatcgacaat tcggcaaggc tacgcgttcg
     9361 cagcatgtcc gcgcctgcct gcagcagggc ggccgtggtc tctcggcttc gcttctgccg
     9421 cgacggcgtc acgccgggaa agtcgaattc tgttgattga ggccgcatcc gatattgcat
     9481 cttcagccgg atctaataat aatggtaatc atagttcaga ttatattttt tgggcaaggc
     9541 tggtcgcatt ttcgctggcc cggctgtgtt cggcgaaccc ggatcagcgc cgtcgtcatt
     9601 gcaagaggag tggattatga gcgcaggcag cgatacgcca tttcgcggca cgatcggcaa
     9661 aacggtcacg gactcgcggc catggtggcc gccgacgcgg cgaccgcccg agggcgcccc
     9721 caatattctc gtggtgctgt tcgacgacgt cggcttttcc gatttcggct gttacggctc
     9781 gccgatcaag acgcctgtta tcgacaggct cgcggccgag ggcctgcgct acagtggatt
     9841 ccacaccacc gcgatgtgct cgaccacgcg cgcggcgctg ttgaccggcc gcaaccacca
     9901 ttccgtcggg gtcggctgcc tcgccaattt cgacagcggt tatcccggct atcgcggcaa
     9961 gatcgcgcgt gaagccggaa cgctggcgga aatgctcaaa gcccacgcct accgcaacta
    10021 catggtcggc aaatggcatg tcacgccact gaccgaaagc ggcgccaccg gaccgtttga
    10081 cggctggccg ctgggccgcg gcttcgaccg gttttatggt ttcctggacg cagagaccga
    10141 tcagttcgcg ccggaattgg tgtcggataa cacccacatc gacccgcccg gaacctatgc
    10201 cagcggctat catctgacgt cggatctggt cgatcagtcg atccgcttca tcgccgacca
    10261 catcgccgac cggcccgatc tgccctggct gacctggctg gcgctgggcg cctgccacgc
    10321 gccgcatcag gcgcccgccg atttcatcaa gggatatgat gagattttcg cgcacggctg
    10381 ggatgtcgag cgcgaacagc gcctggcgcg tcagatcgcg acggggatcg ttccgcccgg
    10441 gaccagaatg cccgaccgca atgacggcgt gaaggcctgg gatgaacata gcctcgatga
    10501 aaagcgcata tttacccggc tgcaatcggc cttcgccggc ctgcttgatc acgccgactg
    10561 gcatctggcg cggctgatcg cgtttctcga gaccgccggc attcgcgaca acaccctgat
    10621 actcgtgctg tcggacaacg gcgccagcca ggaaggcggc ccgctcggct tcgtcaatgc
    10681 gatgggaccg tataatttca ggcctgaacc tgttccggaa aagatgcggc ggctcgacga
    10741 catcggcggt ccggactcgc acaccaattt tccgcacggc tgggcgatgg cttccaacac
    10801 gccgttgcgc cgctacaagc agaacaccca tggcggcggc atccgcgatc cttttgtgct
    10861 ttcgtggccg aagcgcgtgt cggcgaaggg cgaactgcgc catcagttcg tccacgccag
    10921 cgacctcgtg ccgacattgc tcgatttgat cggcatcgcg gcgccggcgg agatcgcggg
    10981 ctgtgcgcaa atgccgatcg agggcgaaag ttttgcatgc tcgattgcgg acgccgccgc
    11041 cccgtcgaaa tcgtcgccac aatatttcga gatgttcggg catcgcggcc tttggcacca
    11101 ggggtggaag gcggtcgcct atcatccgcc agggacgccg ttcgagaacg acaaatggga
    11161 gctgttccat ctggaccggg atttctccga ggtcgacgat ctcgcattga gcgaacctga
    11221 ccgcctggct gacatgatcc aactgtggtg gagcgaggct gaaaagcaca acgtg
//
LOCUS       DATNOI010000050         2929 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1038468,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000050 DATNOI010000000
VERSION     DATNOI010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038468"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /locus_tag="VN968_01745"
     CDS             complement(<1..96)
                     /locus_tag="VN968_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HXL28005.1"
                     /translation="MAKATLTISSKNYSSWSLRGWLMTRFSGLEFD"
     gene            293..1516
                     /gene="phaZ"
                     /locus_tag="VN968_01750"
     CDS             293..1516
                     /gene="phaZ"
                     /locus_tag="VN968_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HXL28006.1"
                     /translation="MMSMLYQAYQNHMDLTAPWRNGAASALKYLNLVPQGVSDRLFGR
                     LAAALELISRTSLTYTRPAYGIDKVLVGNRELEVAEEVTYATPFGSLLHFKKENSPEQ
                     PRLLLVAPMSGHFATLLRGTVQTLLQDHDVYITDWHNPRDIPLDHGKFGLDEYSEHLI
                     AFLEQLGPRAHMVAICQPSVSALAAAAIMCEDNHPARPATLTLMAGPIDTRIQPTKVN
                     QFAKSKPLKWFEENLINYVPLQCKGAYRQVYPGFVQLTAFVSMNLERHIKQHIDLANH
                     LAKGDKEKAEIIKTFYDEYFAVMDLPAEFYIETVRDVFQEHLLPQGKLTFHGRPVNPA
                     SIKRMGLMTVEGEKDDICSIGQTLAAQDLCTGVRAYRKVHHMQAGVGHYGVFSGKRWN
                     NEIYPLLRDFVHVNS"
     gene            1616..2413
                     /locus_tag="VN968_01755"
     CDS             1616..2413
                     /locus_tag="VN968_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXL28007.1"
                     /translation="MRWRLAALRTLALGFAGALLLAGSAGAAEVRVMISGGLTAAYKE
                     LVPEFERSTGNKVLTAYGPSMGTTVNAIPNRLERGEPADVLIMVGYALGDLVKQGMVI
                     ADSRVDLAKSPIGIAVKSGTPKPDISSADAVKRALLAAKSIAYSDSASGVYVSTEMFQ
                     RLGIADAMKDKARKIPAEPVARVVARGDAEIGFQQISELLPVPGIDIVGQLPPELQKI
                     TVFSAGIATVAKEPDAGKALIRFLASPAAGAAIVKSGIEPIPSGATN"
     gene            complement(2433..>2929)
                     /locus_tag="VN968_01760"
     CDS             complement(2433..>2929)
                     /locus_tag="VN968_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL28008.1"
                     /translation="LFAERLADLTKSPTLLGVIAAGVYVFGAMTQYTIGKLIDRYSLK
                     TIVLPLSFLLAPFLYLAATLSNLPLIIAAIGIVMGAFGQVTVNDAMVGKYTSEQWRSR
                     AYAVRYFVGFTAAGASVGLVAWLYEEGGFVTMLHAFSGLCLLVIAAAIILPAEIKMPA
                     AGAD"
BASE COUNT          590 a          935 c          878 g          526 t
ORIGIN      
        1 gtcgaactcc agcccggaaa acctggtcat gagccagccg cgcagcgacc acgacgaata
       61 gttcttgctg ctgatggtga gtgtcgcttt ggccatttgg cttttccctt aacgcccgcc
      121 gggcgcccac tggggccgaa cgggctcagc ttcgcgccaa aacgtcccgc cgcacagtgc
      181 ccattgcttg tgcctcatgc atccactgca gcaagcgacg tgccaatttg caaggctatt
      241 tgactccgct ttggcttcga tattgcatgg caatacgggc ggggcggttc ggatgatgtc
      301 gatgctctat caggcctacc agaaccacat ggacctgacg gctccatggc ggaacggggc
      361 cgcatcggcg ctgaaatatc tcaacctcgt tccgcaaggc gtctccgacc ggctgtttgg
      421 acggctcgcc gctgcgctgg agctgatctc gcgcacgtcg ctgacctata cccgcccggc
      481 ttacggcatc gacaaggtgc tggtcggcaa cagggaattg gaggtggccg aggaggtcac
      541 ctacgccacg ccgttcggct cgctgttgca tttcaagaaa gagaattcgc cggagcagcc
      601 acgcctgttg ctggtggcgc caatgtccgg ccatttcgcg acgctgctgc gcggcaccgt
      661 gcaaaccctg ctgcaggatc acgacgtcta cattaccgac tggcacaatc cccgcgacat
      721 tccgctcgat cacggaaaat tcgggctcga cgaatattcc gagcacctca tcgcctttct
      781 cgaacaactg ggcccgcgcg cgcacatggt cgcgatctgc cagccctcgg tgtccgcgct
      841 cgcggccgcc gcgatcatgt gtgaggacaa tcacccggca cgcccggcaa ccctgacctt
      901 gatggccgga ccgatcgaca cccgaatcca gccgaccaag gtcaaccagt tcgccaagag
      961 caagccgctg aaatggttcg aggagaacct gatcaattac gtgccgttgc aatgcaaggg
     1021 cgcgtaccgc caggtctatc ccggcttcgt gcagctcacc gccttcgtgt cgatgaatct
     1081 cgagcgccac atcaagcagc acatcgacct cgccaaccat ctcgccaagg gcgacaagga
     1141 aaaggctgaa atcatcaaga cgttttatga cgaatatttc gcggtcatgg acctgccggc
     1201 ggaattttat atcgagaccg tccgcgacgt gtttcaggag cacctgctgc cgcagggaaa
     1261 gctgaccttt catggccggc cggtgaaccc ggcttcgatc aagcggatgg gcctgatgac
     1321 ggtcgaaggc gagaaggacg acatctgctc gatcggccag acgctggccg cgcaggatct
     1381 ctgcaccggc gtgcgcgcct atcgcaaggt gcaccacatg caggccggtg tcggccatta
     1441 cggcgtgttc agcggcaagc gctggaacaa cgaaatctat ccgctgctgc gggatttcgt
     1501 gcacgtcaat tcgtgatggg cttcgaaccc atctctcccc gggctaactc cggtttgcgc
     1561 gatacgatcg tgccctcaag gacgactaat caagatcgtc acgggaggat aacccatgag
     1621 atggagactg gctgctcttc gtacgcttgc gctcggcttc gccggcgctc tgttgctggc
     1681 aggctcggcc ggcgccgccg aggtccgggt gatgatctcg ggcgggctga cggctgcgta
     1741 caaggagctt gtacccgaat tcgagcgctc aaccggcaac aaggtgctga cggcatatgg
     1801 cccttcgatg gggaccacgg taaatgcgat cccgaaccgg ctggaacgcg gcgaacccgc
     1861 cgatgtcctg atcatggtcg gctatgcgct cggcgacctc gtcaagcagg gcatggtgat
     1921 cgccgacagc cgcgtcgatc tcgcgaaatc gccgatcggc atcgcggtga aatccggaac
     1981 gccgaagccg gacatctctt ccgccgatgc ggtgaagcgc gcgctgctgg cggcgaaatc
     2041 gatcgcctat tccgacagcg ccagcggcgt ttatgtcagc accgaaatgt tccaacgcct
     2101 cggcattgcc gatgccatga aggacaaggc ccggaaaatt cctgccgagc ccgttgccag
     2161 ggtcgtggcg cgcggcgatg ccgagatcgg cttccagcag atcagcgagc tgcttccggt
     2221 gccgggcatc gatatcgtcg ggcaattgcc gcccgaattg cagaagatca cggtgttttc
     2281 ggccgggatc gcgacagtcg ccaaggagcc cgacgccggc aaggcgctga tcaggtttct
     2341 cgcctctccc gccgccggcg ccgcgatcgt caagagcggg atagagccga tcccgtcggg
     2401 cgcgacgaac tagccggaca caaaagctcg ggctagtccg cccccgccgc aggcatcttg
     2461 atttccgccg gcagaatgat cgccgccgcg atcaccagca ggcacagtcc ggaaaacgca
     2521 tgcagcatcg tgacgaaacc gccctcctcg tagagccacg ctaccaggcc gaccgacgcg
     2581 cccgcggcgg tgaaaccgac gaagtagcgc accgcgtagg cgcgcgagcg ccattgctcg
     2641 ctagtgtatt tgccgaccat ggcgtcgttg accgtcacct ggccaaaggc gcccatcacg
     2701 atcccgatcg cggcgatgat cagcggcagg ttggatagcg tcgccgccag atacaaaaac
     2761 ggcgcgagca ggaacgacag cggcagaaca atggtcttga gcgaatagcg gtcgatcagc
     2821 ttgccgatgg tgtattgcgt catcgcgccg aacacatata caccggccgc gatcacaccc
     2881 aacagtgtcg ggcttttggt gaggtccgcc aaacgctccg caaacaatt
//
LOCUS       DATNOI010000051         3047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2125143,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000051 DATNOI010000000
VERSION     DATNOI010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2125143"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /gene="glcF"
                     /locus_tag="VN968_01765"
     CDS             complement(<1..1173)
                     /gene="glcF"
                     /locus_tag="VN968_01765"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HXL28009.1"
                     /translation="MKTEFSPAQLANPDIAEADKILRACVHCGFCTATCPTYVLLGDE
                     LDSPRGRIYLIKEMLEKDRPPTQEVVKHIDRCLSCLACMTTCPSGVHYMHLVDQARAR
                     IEKEYARPLTERLLRATLAWVLPRPGLFRLSMILARLARPLAALLPTPKAAASPGLVR
                     RIKAMLALAPGGLPAPGPAGGSVFPALGPRRGRVALLQGCAQQVLAPRINQAAIGLLT
                     RHGVEVVLVKGEQCCGALTHHLGRDDDALARARANIAVWLKEAEGDGLDAILVTTSGC
                     GTVIKDYGFMLREDHNFAAAAAKISALAKDITEYLGSIELQPAQQQSDLIIAYHSACS
                     LQHGQKITHAPKELLSKNGFVVKDVPESHLCCGSAGTYNILQPDIANRLRDRKVANI"
     gene            complement(1170..2408)
                     /locus_tag="VN968_01770"
     CDS             complement(1170..2408)
                     /locus_tag="VN968_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXL28010.1"
                     /translation="MDTLKVRDAGDVEQAVRAAIASEQPLEIIGHGSKRLIGHPMATN
                     ALLDLSALNGVTSYEPTELIITVQAGAPLADVKSLIDSKNQQFAFEPIDTSLLLGTPS
                     LGTVGGMIGAGLAGPRRIKAGGARDHLLGVQAVSGFGDSFKAGGRVVKNVTGYDLCKL
                     LAGSWGTLAVMTEATLKVMPRPESERTLVLRGLDDVAANRAMTAALGSPFDVSGAAHV
                     PKSVFRAAMNGLGDLGTPREAVTLLRLEGITASAVHRAAAVGKLLAPFGAAEMAEDTA
                     SQAIWSSIRDVQPFAANGALGAWPVWRIVCPPASGGALGERLARDTGGDVIYDWGGGL
                     IWAALPPRPDAQAALVRQRVNAAGGHAMLLRAPEEIRRNVEVFHPQPGGLAALSERVR
                     HSFDPKSILNRGRMTRESAT"
     gene            2725..>3047
                     /locus_tag="VN968_01775"
     CDS             2725..>3047
                     /locus_tag="VN968_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HXL28011.1"
                     /translation="MDRYAINERTISEAVNDGGLLVVAQWEAKEGQADRIADILSRFL
                     PEAQSEPGAKLFSRAKENPAQFLFYELFRDEAAFKAHQDSAHFKTYIAGEALPLLAKR
                     ERAQYA"
BASE COUNT          554 a         1024 c          940 g          529 t
ORIGIN      
        1 aatattggcg acctttcgat cgcgcaatct gttcgcaatg tcaggctgga gaatgttgta
       61 cgtccccgcc gaaccgcaac acaaatggct ctcgggtaca tctttcacca cgaatccgtt
      121 cttggaaagc aattctttcg gtgcgtgcgt gattttctgt ccgtgctgca gcgaacaggc
      181 tgagtgatag gcgatgatga gatcgctttg ttgctgcgct ggctgcagtt cgatgctgcc
      241 gagatactcg gtgatgtcct tggccagcgc ggaaatcttc gcagccgcgg ccgcgaagtt
      301 gtggtcctcg cgcagcatga agccataatc cttgatcacc gtcccgcaac ccgatgtcgt
      361 caccaggata gcgtcgaggc catctccctc ggcctccttc agccacacgg cgatattggc
      421 gcgcgcccgc gccaatgcgt cgtcgtcgcg tccgaggtgg tgggtcagcg cgccgcaaca
      481 ttgctcgccc ttgaccagca cgacctcgac gccgtggcgc gtcaggaggc caatcgccgc
      541 ctgattgatg cgcggtgcca gtacctgttg ggcacagccc tgcagcagcg cgacccttcc
      601 gcgccgaggg ccgagggccg gaaaaacact tccgccagcc ggtcccggag ccggcaagcc
      661 gcccggcgcc agagccagca tggccttgat tcgccgtact aaacccggcg acgccgcggc
      721 tttaggggtt ggcagaaggg cggccagcgg tcgggccagg cgtgccagga tcatgctcaa
      781 gcgaaacagc cccggccgcg gcagtaccca ggcgagcgtc gcgcgcaaca gacgctcggt
      841 caacggccgg gcatattcct tttcaattct tgcccgcgcc tgatcgacca gatgcatgta
      901 atgcacgccc gatggacagg tggtcatgca ggcgaggcag gagagacagc ggtcgatatg
      961 cttgaccacc tcttgcgtcg gcggccggtc cttttccagc atctccttga tgagatagat
     1021 ccgtccgcgc gggctatcga gttcgtcgcc gagcagcaca taggtggggc aggtcgcggt
     1081 acagaagccg caatgcacgc aggcgcgcaa aatcttgtcg gcttccgcga tatcgggatt
     1141 ggcaagttga gcggggctga attcggtttt catgtcgcgg actcccgcgt catccggccg
     1201 cgattgagga tgcttttcgg atcgaaactg tggcgaacgc gttcgctcaa ggcggcaagg
     1261 ccccccggct ggggatgaaa cacctcgacg ttgcggcgaa tctcctcggg cgcgcgcaac
     1321 agcatagcgt ggccaccggc ggcgttgacg cgctggcgca ccagcgcggc ctgggcgtcg
     1381 ggcctcggcg gcagggccgc ccagatcagg cccccgcccc agtcgtagat cacatcgccg
     1441 ccggtatcgc gcgccagccg ctcgccgagc gcgcctcccg aagccggcgg acaaacgatc
     1501 cgccagaccg gccacgctcc cagggcgccg ttggccgcga atggttgaac atcacgaatc
     1561 gagctccaga tcgcctgaga ggcggtatct tcggccatct ccgcggcccc aaacggcgca
     1621 agcaacttgc cgaccgccgc cgcgcgatgc acagccgacg cggtgatgcc ctccagccgc
     1681 agcagcgtga ctgcctctcg cggcgttccg agatcgccaa gcccgttcat tgcagctcga
     1741 aataccgatt tcggcacgtg cgccgcgccg gatacgtcga acggcgaccc cagcgccgcg
     1801 gtcatcgccc ggtttgcagc gacatcgtcc agcccgcgca gcaccagcgt gcgctcgctc
     1861 tcgggccttg gcatcacctt caacgtcgcc tccgtcatga ccgccagcgt gccccacgac
     1921 cccgccaaga gcttgcacag gtcgtagccg gtgacgttct tcaccacccg gccgccggct
     1981 ttaaagctgt cgccgaagcc cgacaccgcc tgcacgccca ataagtggtc gcgcgcgcca
     2041 ccggccttga tgcggcgggg accggccagg ccagcgccga tcatgccccc gacggtcccc
     2101 aggcttggcg tgcccaggag cagcgaagtg tcgatcggct cgaacgcgaa ctgctgattt
     2161 ttggaatcga tcagcgactt gacgtcggcg agcggcgcgc ccgcctgaac ggtgatgatc
     2221 agctcggtcg gttcatatga cgtcactcca ttgagcgccg acaaatccaa tagcgcgttg
     2281 gttgccatcg ggtggccgat cagccgcttg gagccatggc cgatgatttc cagcggctgc
     2341 tcgctggcta tcgccgcgcg cactgcctgc tcgacatccc ccgcatcgcg tactttcaga
     2401 gtgtccacgc cttcagtccc gcagttcccg ctcgagctgt tgaattggcg atgtttgtcg
     2461 cagccatccg gactccagac catcccttct aaaggcgtgg ccgagaggca caagggtgag
     2521 ctttcacgaa cggcaacgcg acatccgtcg acgggccggg tggccggctc gagagccggc
     2581 gtcatcggct agcactgccg gcctcccgtc gtatcgggca ccctgggccc ggcccgcgcg
     2641 cgacgctctc acttcgaccg ttatcgaagc atccgtgcgc agggcggcgc aatgattgag
     2701 ggcaatctgc aaggagcaag caccatggac cgatatgcaa tcaacgagag aaccatcagc
     2761 gaggccgtca acgacggcgg cctgctggtc gtcgcgcaat gggaagccaa ggaaggacag
     2821 gccgaccgaa tcgccgatat tctgtctcgc ttcctgccgg aggcacaaag cgagcccggc
     2881 gcaaagctgt tttcccgggc gaaggaaaat cccgcacagt tcctgttcta cgagctgttt
     2941 cgcgacgaag ccgctttcaa ggcccatcag gacagcgcgc atttcaagac ctatatcgca
     3001 ggcgaagcgt tgccgctgct cgccaagcgc gaacgcgcgc aatacgc
//
LOCUS       DATNOI010000052         3360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_690972,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000052 DATNOI010000000
VERSION     DATNOI010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690972"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /gene="gcl"
                     /locus_tag="VN968_01780"
     CDS             complement(<1..1622)
                     /gene="gcl"
                     /locus_tag="VN968_01780"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HXL28012.1"
                     /translation="MAKMRAIDAAVRILEKEGVTCAFGVPGAAINPLYSALKKRGSIN
                     HFLARHVEGASHMAEGYTRAKAGNIGVCIGTSGPAGTDMITGLYSAIADSIPILCITG
                     QAPRARLYKEDFQAIDIESIAKPVTKWAVTVREPALVPRVFSQAFHIMRSGRPGPVLI
                     DLPLDVQLAEIEFDDETYEPLPVYKPAATRKQIGKALDMLDAAERPLIVAGGGVINAD
                     ASDLLVAFAEIVNVPVIPTLMGWGAIPDDHVLMAGMVGLQTSHRYGNATMLESDFVLG
                     IGNRWANRHTGSIETYTKGRTFVHVDIEPTQIGRVFNPDLGIVSDAKAALELFVTVAK
                     ERLKSGKLKKRQAWPAACQDRKRSMLRKSHFDNVPIKPQRVYEEMSKAFGRDTCYVSV
                     IGLSQIAGAQFLGVYHPRNWINAGQAGPLGWTLPAALGVRAADPDREIVALSGDYDFQ
                     FLIEELAVGAQFKLPYIHVVVNNSYLGLIRQAQRGFDMDYHVQLSFENINAPELGVYG
                     VDHVAVAEGLGCKAIRVTDPRDSQAAFAAAREL"
     gene            complement(1751..2653)
                     /locus_tag="VN968_01785"
     CDS             complement(1751..2653)
                     /locus_tag="VN968_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HXL28013.1"
                     /translation="MKRDVIRRKAIEPKPNAEHDADSRDGGVQSVDRALSIIETLAED
                     DEGYRLSDLAIRTGLSTSTVHRLLATLEKRRFVQFDRYESKWHVGAQSFSVGATFARR
                     RNFVAQAMPYLRKLRDQTRETANLAVVDDESIIVLTRMESREIMRSLTKVGGRVAMVA
                     SGVGKAVLATYSDEDVNAIIHRQGMPRLTEKSIVRPGELFRELEAIRRQGYAVDDEEA
                     RMGLRCVAAVVYNDCSEPLAAISVSGMTSRLTDDRLPALGRIVHEVAAELTVVLGGVL
                     PVAADEGSAKRGFGHSNSSTFSFR"
     gene            complement(2932..>3360)
                     /locus_tag="VN968_01790"
     CDS             complement(2932..>3360)
                     /locus_tag="VN968_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXL28014.1"
                     /translation="DNGLSLMTMPPDLDAAFRKFIPPFGAAGNPVDITGGEPPITYVN
                     TVKLGLSDERIHSLILGYWHTIVTPPMVFARNMVEVKKEMEAKGFVKPMVASLAGDVE
                     VEEAAEYLYQNGIPAYAYSTELPVEVLGAKYKWARGAGLL"
BASE COUNT          646 a         1036 c         1020 g          658 t
ORIGIN      
        1 agctcgcgag cggccgcgaa cgccgcctgc gaatccctgg gatcggtgac gcggatcgcc
       61 ttgcagccga ggccttcggc gaccgcgacg tgatcgacgc catagactcc aagttcgggt
      121 gcgttgatgt tctcgaacga cagctgcaca tgatagtcca tgtcgaaacc gcgctgtgcc
      181 tgacggatca gcccgagata cgagttgttc acgacgacgt ggatataggg caacttgaat
      241 tgcgcgccga ccgcaagttc ttcgatcagg aactgaaaat cgtaatcgcc tgagagcgcc
      301 acgatctccc gatcgggatc ggcggcgcgc acgcccagcg ccgcgggaag cgtccagccc
      361 agcggcccgg cctgcccggc attgatccag ttgcgcgggt ggtagacgcc aaggaattgc
      421 gcgccggcga tctgcgacaa cccgatcacg ctgacatagc aggtatcgcg gccgaacgcc
      481 ttgctcattt cttcataaac gcgctgcggc ttgatcggca cgttgtcgaa atgactcttg
      541 cgcagcatcg agcgcttgcg atcctggcac gccgcgggcc aggcctgccg cttcttcagc
      601 ttgccggatt tcagccgttc ctttgcgacc gtgacgaata attccagcgc cgccttggcg
      661 tcggagacga tgccgagatc gggattgaag acgcgtccga tctgggtcgg ctcgatatcg
      721 acatgcacaa aggtacgccc cttggtgtag gtctcgatcg agccggtgtg ccggttggcc
      781 cagcggttgc cgatgccaag cacgaagtcg gattccagca tggtcgcatt gccgtagcga
      841 tggctggtct gcaatccgac catccccgcc atcagaacgt ggtcatccgg gatggcgccc
      901 cagcccatca gcgtcggtat caccggtacg ttgacgatct cggcgaaggc cacgagaagg
      961 tcggaggcat ctgcattgat gacaccgccc cccgccacga tcagcggacg ctccgcggca
     1021 tcgagcatat cgagcgcctt cccgatctgc ttgcgggtgg cggccggctt gtagacgggc
     1081 aacggctcat aagtttcgtc gtcgaattcg atttccgcaa gctgcacatc gagtggaagg
     1141 tcgatcagca ccggacccgg acgccccgaa cgcatgatgt gaaaggcctg gctgaagacg
     1201 cgaggcacca gcgccggttc gcgcacggta accgcccatt tcgtcaccgg cttggcgatc
     1261 gactcgatat cgatggcctg aaaatcttcc ttgtacagac gcgcccgcgg cgcttgcccg
     1321 gtgatgcaaa ggatcggaat cgaatcggcg atcgcagaat agaggccggt gatcatgtcg
     1381 gtgccggccg ggcctgacgt gccgatgcag acgccgatgt tgcccgcctt ggcgcgggta
     1441 tagccctccg ccatatgcga ggcgccctcg acgtggcgcg ccagaaagtg gttgatcgaa
     1501 ccgcgcttct tcaaggctga atagagcgga ttgatcgcgg cgccgggaac gccgaacgcg
     1561 caagtgacgc cttccttttc gagaatacgt acggcagcat cgattgcccg catcttcgcc
     1621 atgtgaccgc ccgttcattc tgtgtttgct cgcgggcaat catcacgcga acgacgttgg
     1681 gtcacaacga aactgatttt atttcccgcg atgcggcact ccgccggatt tcccacgctt
     1741 gttcaggcga ttagcggaaa gaaaaagtgg aagaattgct gtggccaaat ccacgcttcg
     1801 ctgagccttc atccgccgcg accggcaaga cgccgccgag cacaacggtc agctcagctg
     1861 ccacctcatg cacaatcctg ccgagagccg gcaatcggtc gtcggtcagc cggctggtca
     1921 tgcctgagac ggaaattgcc gcaagcggtt cgctgcaatc gttgtagacc acggcggcga
     1981 cgcaccgcag ccccatacgt gcttcctcat catcgacggc gtaaccctgt cgccggatcg
     2041 cttcaagttc cctaaacagt tcgccgggcc gcacgatcga cttttccgtc aaacgcggca
     2101 tgccttgccg gtggatgatc gcgttgacgt cttcatcgga ataggtagca agcaccgcct
     2161 tgccgacgcc ggacgccacc atcgcgacgc ggccgccgac cttggtgagc gaacgcatga
     2221 tctcgcggct ttccatgcgg gttagcacga tgatcgattc atcatcgacg accgcaaggt
     2281 ttgccgtttc tctggtctgg tcgcgcaact tgcgcaaata cggcatggcc tgcgccacga
     2341 agttgcggcg acgggcgaag gtggccccga cggaaaaact ctgcgcgccg acatgccact
     2401 tcgattcata gcgatcgaat tgcacgaagc ggcgcttttc cagtgtcgcc agcaggcgat
     2461 gaacggtcga ggtcgacaag ccggtgcgga tggcgagatc gctcaggcga tagccctcgt
     2521 cgtcttcggc aagcgtttcg atgatcgata gcgcgcgatc gacggactgc acgccaccgt
     2581 cgcgggaatc ggcgtcatgt tcagcgttgg gtttcggctc gatggccttg cgccggatca
     2641 cgtctctctt catcgcgacc tgcattcatc cgttagtcag actgctagcg cgtattccac
     2701 ataggttgga tcaataccgc gtatccgatg gcctcgtggt tcgagacgcg cggccttgcc
     2761 gcgctcctca ccatgagggt ctaagacctc atcctgagga gccacgcgta gcgtggcgtc
     2821 tcgaaggata aagccagaaa aatgctccaa gactttggtc ggcaaaatcc tggttcgtta
     2881 gatcaagaat gccgctccgg gtgttttccg gagcggcact tgtcgttggt atcagagcag
     2941 gcccgcgccg cgcgcccatt tgtatttggc ccccaacacc tcgaccggca attctgtcga
     3001 ataggcatag gccgggatgc cgttctggta gagatattcg gcagcctcct cgacctcgac
     3061 gtcgccggcg agcgaggcga ccatcggctt cacgaagccc tttgcctcca tctccttctt
     3121 cacctcgacc atgttgcggg cgaacaccat cggcggcgta acgatggtgt gccagtagcc
     3181 gaggatcagc gaatggatgc gctcatcgga caggccgagc ttgacggtgt tgacgtaagt
     3241 gatcggcggc tcgccaccgg tgatgtcgac cgggttgccg gcggcgccga acggcggaat
     3301 gaacttgcgg aacgcggcgt cgaggtccgg cggcatcgtc attaatgaga gaccgttgtc
//
LOCUS       DATNOI010000053         7247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_533606,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000053 DATNOI010000000
VERSION     DATNOI010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533606"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..415
                     /locus_tag="VN968_01795"
                     /pseudo
     CDS             19..415
                     /locus_tag="VN968_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
     gene            complement(552..2522)
                     /locus_tag="VN968_01800"
     CDS             complement(552..2522)
                     /locus_tag="VN968_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL28015.1"
                     /translation="MLGFAVVLAISAASMGIAYLGFERVSSGVASYRHSVSEGDLAHN
                     IDRELVSYRGLARYYVVTGKEDDGKAALAAEDSLKDAIDQSMKGTTNPAGLDQLTRLA
                     REFRAFTKIFAEILKVKAESALVAQNQLTRSGTMLRFKLDDLPSNADDTELQAIEFGA
                     KKVAAQFQTVTALANTFVINADQTVAASALARIKFVENSLHAIPSTDEKIVQGLKEDA
                     TLLDEYRQALTKLIENSKSIDELTTEMTESAAAIMHGSGAMKAALLSDQQRLESESDA
                     TIGETERLIVMLAAGGFLLGSVLALMLGKGISRPMTAMCKAMRELAGGNFDVVLPGLG
                     RSDELGEMASAVEEFKVQAIAKAERDAAAHDAQNKASSAARRSELIRFADDFEAAVGT
                     IVSNVSASAVQLEQAAGTLTRTAETTQSLSSQVAGASEEASSNMQSVATATEELSATV
                     DEIGTRVRESNRIAEAAVLQAQQTDGRIGKLSRAAQEIGDVVKLITAIAEQTNLLALN
                     ATIESARAGEAGRGFAVVASEVKSLASQTAKATDEISSHILGMQDATQESVAAIKEIG
                     GTIAQISNIASSIASAVEQQSSATQEIARSVQSVAQGTREAAANIMQVNRGATETGSA
                     SEDVLNSARSLSTESARLREELDRFMGNIRAA"
     gene            complement(2798..3502)
                     /locus_tag="VN968_01805"
     CDS             complement(2798..3502)
                     /locus_tag="VN968_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HXL28016.1"
                     /translation="MTAKAHHPLTREQMAWRAAQDLAEGAYVNLGIGIPTLAASFVPP
                     GREVIFHSENGILGMGPKPEPGKEDLYMVDAGKNLTTLVPGGCFVHHADAFLMIRGGH
                     LDVSLLGAFEVSEKGDLANWTTEDPAFPPGIGGAMDLAVGAKEIRIIMEHTSKTGEPR
                     ILTHCTYPLTAAGCVKRIYTNLAVIDVTPHGLVVIEMIPGMTLEKLQALTEPKLQLAP
                     NWKALTPPEAPEARAA"
     gene            complement(3499..4167)
                     /locus_tag="VN968_01810"
     CDS             complement(3499..4167)
                     /locus_tag="VN968_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HXL28017.1"
                     /translation="MDKRVKTTADAVAGVRDGSTVLVAGFGAVGVADHLLDALHEHGA
                     RELTIVANNSGNGDYGLARLINSGRVRKVICSYPRSGDYSAFLKAYRARSLELELVPQ
                     GTISERMRCHAAGLGGFFSPVSAGTKLAEGKETREIDGRLHVFEKPLRGDVALLKANK
                     ADRWGNLTYIKSARNFNPVMAMAADLTVVEVDRIVELGEMDPEAVVTPSIFVDRIVVV
                     GAQA"
     gene            4447..4932
                     /locus_tag="VN968_01815"
     CDS             4447..4932
                     /locus_tag="VN968_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL28018.1"
                     /translation="MSTIKLTVNGKAVSAEVEDRTLLVQLLRENLNLTGTHVGCDTSQ
                     CGACVVHIDGRAVKSCTVLVGQANGANVTTIEGISKGDELHPMQAAFRDNHGLQCGYC
                     TPGMIMSAIDIVHRHGGGLDEDTVRHELEGNICRCTGYHNIVKSVLDAAGRMKVSQAA
                     E"
     gene            5025..>7247
                     /locus_tag="VN968_01820"
     CDS             5025..>7247
                     /locus_tag="VN968_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXL28019.1"
                     /translation="MGVEGIGASVVRKEDRRFITGRGRYVDDIKLQGMTFAQFVRSPH
                     AHAKVKSIDSSEAMKMPGVVGILTGQQIVDDKVGNLICGWAITSKDGTPMKMGAWPAM
                     APETVRFTGQAVAVVIAETKNQAKDAAEAVVVDYEVLPAAADISAAIKPGAPQLHPEA
                     PGNVIYDWAIGDEAAVKDAFSRAANVVSLDLANNRLVPNAMEPRAAIAEYSEAEEHFT
                     LYTTSQNPHVARLVLSAFYNIAPEHKLRVVAPDVGGGFGSKIYIYPEEMVALWASKKV
                     RRPVKWTGDRSEAFLTDAHGRDHITKAEMAFDKDNKIIGLRVKTHANLGAYMSLFSSA
                     VPTYLYATLLSGQYNIPAIHAEVVTVYTNTVPVDAYRGAGRPEASFLLERLMETAARQ
                     LKVDPAELRRKNFITSFPHQTPVIMAYDIGDFGASLDAAMKAIDYAGFPARKAKAKSD
                     GKLRGIGFSCYIEACGIAPSKAVGSLGAGVGLWESAEVRVNPVGTIEILTGSHSHGQG
                     HETTFAQLVAERLGVPISQVQIVHGDTDKVQFGMGTYGSRSIAVGGTAIIKAMEKVEA
                     KGKKIAAHLLEASEGDIVIENGEFKVTGTDKSIALPMVALAAYTAHNLPDGMEPGLKE
                     TAFYDPTNFTFPAGAYICELEVESGTGKTSFVNFVAADDFGRLINPMIVEGQVHGGLA
                     QGIGQALLEGAVYDSSGQLVTASFMDYTMPRADDLPSFKLSHTTTLCPGNPLGVKGCG
                     E"
BASE COUNT         1392 a         2294 c         2234 g         1327 t
ORIGIN      
        1 aacggcatgg agttgatcat ggctgagcat cgcaagaccg cgcgcaaggc cgcaaccctc
       61 aaaagcccgg ctaagcatac gcgaaagccg gcgacgcgga cgacaggcac gacacaaaga
      121 aaggcatcgt ccaaacaggc atcgcccaag cgatggtcgc agcgcgtcac aaaggaaagc
      181 gacgcactgg atctgaagca tggcgtcttc aaactgaccg acccgaagaa aatcgcggcg
      241 tcgctgaagc gctcggcgga acgaagttcg cgccgcaaga ccggcgcctt tcgctcggcg
      301 ctgtcgatgc tgacgtttta tatcaacccg ggccggcaag acgctgccga aaacccaacg
      361 cgaccggctg cagcgcgcca agaccgagct gaggcggcag tttcatcgcg agtgatcctc
      421 agcgtcgtcc cggcgaacgc cgggacccat aacccgcaac gctgattgtt acagaaggcc
      481 tcgatcccgt tgcctaaacg agaatcctcg acgtatgggt cccggcgttc gccgggacga
      541 cgcaggagga atcacgccgc gcggatgttg cccatgaagc ggtcgagttc ctcgcgcaag
      601 cgcgcgcttt cggtcgacaa cgatcgcgcc gaattcaaca cgtcttcgga cgccgagccg
      661 gtttccgtgg cgccgcgatt cacctgcatt atattggcgg cagcttcacg ggttccctgg
      721 gcgacgctct gaacgctgcg ggcgatttcc tgggttgccg agctttgttg ctccaccgcg
      781 ctcgcaatcg acgacgcgat gttcgatatc tgggcgatgg tgccgccaat ctccttgatg
      841 gcggcaaccg attcctgtgt cgcgtcctgc atgccaagaa tgtgagacga tatctcgtcg
      901 gtcgccttcg cggtctggct ggccagcgat ttgacctcgg aagcgacgac ggcgaaacct
      961 cgacccgcct cgccggcacg ggcggattcg atcgttgcat tcagcgccag cagattggtc
     1021 tgctcggcga tcgccgtgat cagcttgacc acgtcgccta tctcttgcgc cgcgcgcgac
     1081 aatttgccga tgcgcccatc ggtttgctgc gcctggagga cggccgcctc ggcgatccgg
     1141 ttggactcac gtacccgtgt gccgatttca tcgacggtgg cggaaagctc ctccgtggcg
     1201 gtggcgaccg attgcatgtt gctcgacgcc tcctccgatg cgcccgcgac ctggctggac
     1261 aggctctgcg tggtttccgc ggtccgcgtc aacgtgccgg cagcctgttc gagctgaacg
     1321 gccgatgccg aaacgttcga gacgatcgtg cctaccgccg cctcgaaatc gtccgcaaag
     1381 cgaatgagct cgctgcggcg tgccgcgctg ctcgctttgt tctgggcatc atgggccgcg
     1441 gcatcgcgtt cggctttggc gatggcctgc accttgaact cttccaccgc gctcgccatc
     1501 tcgccaagtt catcgctgcg gccgaggccg ggcagcacga catcgaaatt gccgccggcg
     1561 agttcgcgca tggccttgca catcgccgtc atcgggcgcg aaattccctt gccaagcatc
     1621 agcgccagca cgctgccgag caggaagccg ccggccgcga gcatcacgat cagccgctcg
     1681 gtctcgccga tggtcgcgtc cgactccgat tcaagccttt gttgatcgga aagcagcgcc
     1741 gccttcatcg cgcccgagcc gtgcatgatg gcggccgccg actcggtcat ttcggtcgtc
     1801 agctcatcga tcgatttcga attctcaatc agttttgtca gggcttgccg atactcatca
     1861 agcaatgtcg cgtcctcctt gaggccctgc acgatctttt catcggtcga cggtatcgcg
     1921 tgcagcgagt tttcgacaaa cttgatgcgc gccagcgcgc tggcggccac cgtctggtcg
     1981 gcgttgatga cgaaggtgtt ggcgagcgct gtcactgttt gaaattgcgc cgccactttt
     2041 ttcgcgccga attcgatcgc ctgaagctcc gtgtcgtcgg cgttgctggg gagatcgtcg
     2101 agcttgaatc gcaacatggt gccgctgcgc gtgagctggt tttgcgccac cagcgcgctc
     2161 tcggccttga ccttgagaat ctcggcaaaa atcttcgtga aggcgcggaa ttcgcgggcg
     2221 aggcgcgtga gctgatcgag gcccgccgga ttggtggtgc ccttcatcga ttgatcgatg
     2281 gcgtccttga gactgtcttc ggctgccaac gccgccttgc cgtcgtcctc cttgcccgtg
     2341 acgacatagt aacgcgccag cccccggtac gagaccagct cgcggtcgat attgtgcgcc
     2401 agatcgccct ccgacacgct gtgtcggtag gacgccacac ctgaggagac gcgctcgaat
     2461 ccaagatagg cgatgcccat gctggcagcc gagatcgcca gcacgacggc aaagccaagc
     2521 atgacttttg cgcgaaagcg caaggtcgga aattttggcg ataaagatcg cggccagaca
     2581 cgtccagaca atcgcacctc gacccccgtc ttcttttcaa cagccagcaa tacccgggaa
     2641 cgtgagttcc cgggagccga tgccaaaaat tagagcagaa tggataaagg gccgtaaatt
     2701 tgcgccggct tttccccgtc atggccgggc atagccgtcc gaaggacggc gtcgcttccg
     2761 ctcgccaatg cccgggcata acgaacataa ggagcgccta cgccgcgcgg gcctcgggcg
     2821 cctcgggcgg cgtcagggcc ttccaattgg gggccagttg cagcttcggc tcggtcagcg
     2881 cttgcagctt ctcaagcgtc atgcccggaa tcatttcgat cacgaccaat ccgtgcgggg
     2941 tcacatcgat caccgcgagg ttggtgtaaa tgcgcttgac gcagccggcc gcggtgagcg
     3001 gataggtgca gtgcgtcagg atacgcggct cgcccgtctt gctggtgtgc tccatgatga
     3061 tgcggatctc cttggcgccg accgcgagat ccatggcgcc gccgatacca ggcgggaatg
     3121 ccggatcctc ggtggtccag ttcgcaagat cgcccttttc ggagacctcg aaggcgccga
     3181 gcaggctgac atcaagatgg ccgccgcgga tcatcaaaaa cgcatcggcg tgatggacga
     3241 agcaaccgcc ggggaccagc gtcgtgaggt tcttgccggc gtcgaccata tagaggtctt
     3301 ccttgccggg ctccggcttt ggacccatgc cgaggatgcc gttctcgctg tggaagatca
     3361 cctcgcgccc cggcggcaca aagctcgccg ccaaggtcgg aatgccgatg ccgagattga
     3421 cataggcgcc ttccgccaaa tcctgcgcgg cgcgccatgc catttgttca cgcgtcagag
     3481 ggtggtgggc tttggcggtc atgcctgcgc tccgacaacg acgatgcgat cgacaaaaat
     3541 gctcggggtg accaccgctt cgggatccat ttcgccaagc tccacgattc tgtcgacttc
     3601 cacgacggtc aaatcggcgg ccatcgccat cacggggttg aaattccgcg ccgactttat
     3661 gtaagtaaga ttgccccagc ggtcggcctt gttggccttg agcagcgcaa catcgcccct
     3721 cagcggcttc tcgaacacgt gcagccggcc atcgatttcc cgagtttcct tgccctcggc
     3781 gagcttggtc ccggccgaaa ccggcgagaa aaacccgcca aggccggccg catggcagcg
     3841 catgcgttcg ctgatggtgc cttgcgggac caattccagc tcaaggctcc tggcccgata
     3901 ggctttcaga aaggcgctgt agtcgcccga gcgcggatag gagcagatca ccttgcgcac
     3961 gcgcccggaa ttgatcagcc gggccaggcc gtaatcgccg ttgccggagt tgttggcgac
     4021 gatggtcagc tcgcgggcgc cgtgttcgtg aagtgcatcg agcagatggt cggcgacccc
     4081 gacggcgcca aatcctgcca ccaataccgt cgatccatcc cgaaccccgg ctacggcgtc
     4141 ggcggtggtc ttgacccgct tgtcgatcac gtcaaagggc tccttttggg cggcattgtc
     4201 gtttggtgtc cgtggcttat gcctggatca accggccggg aatagatcac caactccggc
     4261 gccgagaaag cctcaaattt gcagccgtga tccgcatcga gcccctgcgg tcgtccgggc
     4321 gaatgccggg atgacagatg taacgcaaat ggcagcaccg tgaccttcgc gacgaaaatc
     4381 ctagttgcaa agtccctccc ccgtgcctct aattggaata attaaaagca tcgggaggta
     4441 tcaagcgtgt ctaccatcaa attgacggtg aacggtaaag cggtctcggc cgaggtcgag
     4501 gaccggaccc tcctggtcca gctcctgcgc gagaatttga atctaaccgg cacccatgtc
     4561 ggctgcgaca ccagccaatg cggcgcctgc gtcgtgcata tcgatggccg ggcggtcaaa
     4621 tcatgcaccg tgctggtcgg acaggccaac ggcgccaacg tcaccaccat cgagggcatc
     4681 tcgaagggag acgaactgca cccgatgcag gccgcgttcc gcgataatca tgggctgcag
     4741 tgcggttact gcacgccggg catgatcatg tcggcgatcg acatcgtcca ccgccacggc
     4801 ggcggtctcg acgaggacac cgtccggcac gaacttgaag gcaatatctg ccgctgcacc
     4861 ggctaccaca atatcgtgaa atcggtgctc gacgccgctg gccgcatgaa ggtttcgcag
     4921 gcggcggaat agccgtcgcc gacgttcgat tcaatcatca gatttaggcc gccgaatttc
     4981 gatggaaatg gcgggcaaca aaactccggc agggagatca ggacatgggc gttgaaggta
     5041 ttggcgcaag cgtcgtgcgc aaggaagacc gacgtttcat cacaggccgc ggccgctacg
     5101 tcgacgacat caagctgcag ggcatgactt tcgcccagtt cgtccgcagc ccccacgcgc
     5161 acgccaaggt gaagagcatc gactcgtcgg aggcgatgaa gatgcccggc gtggtcggga
     5221 tactgaccgg acaacagatc gtcgacgaca aggtcggcaa tctcatttgt gggtgggcga
     5281 ttacctccaa ggacggcacg ccgatgaaaa tgggcgcgtg gccggcgatg gcgccggaga
     5341 ccgtgcgctt taccggacag gcggtcgcgg tggtgattgc cgagaccaag aaccaggcca
     5401 aggacgcggc ggaagccgtc gtggtcgact acgaagtgct tcccgcagcc gccgatatca
     5461 gcgcggcgat caagccgggc gcgccgcagt tgcatccgga agcacccggc aacgtgatct
     5521 acgactgggc gatcggcgac gaggccgccg tcaaggacgc cttcagcagg gcggccaatg
     5581 tggtgtcgct cgacctcgcc aataaccggc tggtgccgaa tgcgatggag ccgcgcgcgg
     5641 cgatcgcgga atacagcgag gccgaagagc attttaccct ctacacgacc tcgcagaatc
     5701 cgcatgtcgc ccggctcgtg ctgtcggcgt tctacaacat cgcgccggaa cacaagctgc
     5761 gggtggtcgc gcccgatgtc ggcggcggct tcggctccaa gatctacatc tatcccgaag
     5821 agatggtggc gctgtgggcc tccaagaagg tacggcgtcc agtgaagtgg accggcgatc
     5881 gctccgaggc cttcctcacc gacgcccatg gtcgcgatca tatcacgaag gcggagatgg
     5941 ccttcgacaa ggacaacaag attattggcc tgcgcgtgaa aacccacgcc aatctcggcg
     6001 cctatatgtc gctgttctcg tcggcggtgc cgacctatct ctacgcgacg ctgctgtctg
     6061 gccagtacaa tatcccagcg atccatgccg aggtggtaac ggtctatacc aacaccgtac
     6121 cggttgatgc ctaccgcggc gcaggccgtc ccgaggcgag tttcctgctc gaacgcctga
     6181 tggaaaccgc ggcgcggcaa ttgaaggtcg atcccgcgga attgcgccgc aagaatttca
     6241 tcaccagctt cccgcaccag accccggtga tcatggccta cgacatcggc gatttcggcg
     6301 cgtcgctgga tgccgccatg aaggcgatcg actatgccgg ctttccggca cggaaggcga
     6361 aagccaagtc tgacggcaag ctgcgcggca tcggcttctc ctgctatatc gaggcctgcg
     6421 gcatcgcgcc gtcgaaagcg gtcggcagcc tgggcgccgg tgtcggcttg tgggaatcgg
     6481 cggaggtccg cgtcaatccg gtcggcacca tcgaaatcct caccggatcg cacagccacg
     6541 gccagggcca cgagacgacg tttgcgcaac tggtcgcaga gcggctcggc gttccgatca
     6601 gtcaggtgca gattgtccac ggcgacaccg acaaggtgca gttcggcatg ggcacttatg
     6661 gctcgcgctc gatcgcggtc ggcggcaccg cgatcatcaa ggcgatggag aaggtcgaag
     6721 ccaagggcaa gaaaatcgcg gcgcatctct tggaggcgtc cgagggcgac atcgtcatcg
     6781 agaacggcga attcaaagtc accggcaccg acaagtcgat cgcgctgccg atggtcgcac
     6841 tggcggccta caccgcgcac aatctgcccg acggcatgga gcccggcctg aaggagaccg
     6901 cgttctacga tcccaccaac ttcaccttcc cggcaggcgc ctatatctgc gagctcgagg
     6961 tcgaatccgg caccggcaag acctccttcg tcaatttcgt cgccgccgac gatttcggcc
     7021 ggctgatcaa cccgatgatt gtcgagggcc aggtccatgg cggcctcgcc cagggcatcg
     7081 gccaggcgct gctcgaaggc gcggtttacg actccagcgg ccagctcgtg accgcgtcgt
     7141 tcatggacta caccatgccg cgcgccgacg acctgccgtc gttcaaactg tcgcacacca
     7201 cgacgctgtg cccgggcaat ccgctcggcg tcaaaggttg cggcgag
//
LOCUS       DATNOI010000054         4105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1414080,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000054 DATNOI010000000
VERSION     DATNOI010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414080"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VN968_01825"
     CDS             complement(<1..255)
                     /locus_tag="VN968_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXL28020.1"
                     /translation="MKTEFSLAQLADPDIAEADKILRACVHCGFCTATCPTYVLLGDE
                     LDSPRGRIYLIKEMLEKNRPPTQEVVKHIDRCLSCLACMTT"
     gene            complement(252..1502)
                     /locus_tag="VN968_01830"
     CDS             complement(252..1502)
                     /locus_tag="VN968_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXL28021.1"
                     /translation="MDTLKVRDAGDVEQVVRAALASEQPLEIIGHGGKRLIGQPMATN
                     ALLDLSALNAVTAYEPNELIITVQAGAPLADVLSLIDSRNQQFAFEPVNTSQLLDTPD
                     TGTIGGMIGAGLAGPRRIRAGGARDHLLGAHAVSGFGDSFKAGGRVVKNVTGYDLCKL
                     LAGSWGTLAVMTEVTLKVMPRPESERTLMLRGLDDITANRAMTAALGSPFDVSGAAHV
                     PNSAFRAGAGASADPLAELGSPREAVTLLRLEGITASAVHRAAALAAVLAPFGTAEIL
                     PDAASAAVWSSIRDVQPFAANGSLGGWPVWRIVCPPAAGGALGELLARDTGGDVIYDW
                     GGGLIWAALPPRPDAQAALLRQRVDAVGGHATLIRASEQVRRDVDVFHPQQPGLAALG
                     ERVRHSFDPKNILNRGRMARGSAT"
     gene            complement(1629..2318)
                     /locus_tag="VN968_01835"
     CDS             complement(1629..2318)
                     /locus_tag="VN968_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXL28022.1"
                     /translation="MPTIAPQLAEVAQLVGDPGRANILSSLMDGRALTASELASVAGV
                     TAQTASSHLAKLVKRELLSVEKQGPRRFYRLATPLVAQMLEGMMTVAATGPPRFRPPS
                     RIDSDMRRARTCYDHLAGELGVALTDAMIERRYVVLDADAGELTTEGSDFLANLGIGP
                     TLSAASRRAFCRPCLDWSERRLHLAGRVGAALAYLSFERDWIRRRQVGRSVEITESGS
                     LAFRKLFGARI"
     gene            2360..3022
                     /locus_tag="VN968_01840"
     CDS             2360..3022
                     /locus_tag="VN968_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HXL28023.1"
                     /translation="MTFPAEVSIPIEAYGEILRPRSNRNSGNIDATSRFPVTETERME
                     KGSMEKIPIEKAMEHSNIGTSEPQPTDMFTRRSAINVFGTVIAMVNLSSLAISPSQAQ
                     TEGAMTMGQYAMNKKTVSDAVSGGGLLVVAQWEAKDGQADKVADILRRFLPEAQSDPG
                     AKLFLISRGRENPAQFLFYELFRDEAALKAHQESGYFKTFIAGEALPLLSKRERAQYA
                     LL"
     gene            3494..3916
                     /locus_tag="VN968_01845"
     CDS             3494..3916
                     /locus_tag="VN968_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28024.1"
                     /translation="MADTDPPKEAPIQERRFDETEVDAAKVVTFTEITPIKGAAFAAQ
                     TDLLPAHLGLDLKGGPIVEHDVFASIYNPGKLALLVAWKDPRAANAWSPRKIDGIERL
                     RHRKIRIVRDYGRFDRREAPQFYPDVKGAETKHPEPAH"
BASE COUNT          826 a         1272 c         1275 g          732 t
ORIGIN      
        1 ggtggtcatg caggcaaggc aggagaggca gcggtcgatg tgcttgacca cctcctgcgt
       61 cggcggccgg ttcttttcca gcatttcctt gatcagatag attcggccgc gcgggctatc
      121 gagctcgtcg ccgagcagca cgtaggttgg acaggtcgcg gtgcagaacc cgcaatgcac
      181 gcacgcgcgc aagatcttgt cggcttccgc gatgtcggga tcggcgagtt gggcgaggct
      241 gaattcggtt ttcatgtcgc agatcctcgc gccatccggc cgcgattaag gatatttttc
      301 ggatcgaaac tgtggcggac gcgttcaccg agcgcggcaa ggccaggctg ctgtggatga
      361 aacacatcga catcgcgtcg tacctgctcg gaggcgcgaa tcaatgtggc atggccgccg
      421 acggcgtcga cgcgctgacg cagcagggca gcctgggcgt cgggtctggg tggcagcgcc
      481 gcccagatca agccgccgcc ccagtcatat atgacgtcgc cgccggtgtc gcgcgccaga
      541 agctcgccga gcgcgccgcc ggcggccggc gggcagacga tccgccacac cggccatccc
      601 cccagtgaac cattggcggc gaacggttga acgtcccgaa tcgaactcca caccgccgcg
      661 gaggcggcat ctgggagaat ttccgccgtc ccgaacggcg cgagaaccgc ggccagcgca
      721 gcggcgcgat ggacagccga cgcggtaatg ccttccagcc gcaacagggt gacggcctct
      781 cgcggcgatc ccaattcggc caatgggtcg gccgatgccc ccgccccggc ccgaaacgcc
      841 gaattcggca cgtgcgccgc cccggacaca tcgaatggcg agcccagcgc cgccgtcatc
      901 gcccggttgg cggtgatgtc atccaacccg cgcagcatca gcgtacgttc gctctcgggc
      961 cttggcatga ccttcaacgt cacttcggtc atgaccgcga gcgtgcccca ggaccccgcc
     1021 agcaatttgc agagatcgta accggtgacg ttcttcacca cccggccgcc ggccttgaaa
     1081 ctgtcgccga aacccgacac cgcatgcgcg cccaggagat ggtcgcgggc gccgccggcc
     1141 ctgatgcggc ggggccccgc aaggccggcg ccgatcatgc cgccgatggt tccggtgtcg
     1201 ggcgtgtcca gcagctgcga ggtgttgacc ggctcgaacg cgaactgctg attcctggaa
     1261 tcgatcagcg acaacacgtc ggcgagcggc gcgcccgcct ggacggtgat gatcaactcg
     1321 ttcggctcat aggccgtcac cgcattcagc gccgacaaat ccaacagcgc attggtcgcc
     1381 atcggctggc cgatcagccg cttgccgcca tggccgatga tctccagcgg ctgctcgctg
     1441 gcaagtgcgg cgcgcaccac ctgctcgaca tccccggcgt cgcgtacttt tagagtgtcc
     1501 acgccacccg cccttgtttt cgccgcacga ccaacgccga ggtagccggt cttgtagcgg
     1561 ccatgatatc tcaggggatg tttaaagacg tggatgcgcg gcacaaggcc ggaggcccga
     1621 gttcagcgtc aaattcgtgc accgaacagt tttctgaatg ccaagctgcc gctttcggta
     1681 atttcgaccg atcggcccac ttgtcgcctg cggatccagt cacgctcgaa gctgagatag
     1741 gcgagcgctg cgccgactct gccggccaga tgcaggcgcc gttcgctcca atcgagacat
     1801 ggccggcaga acgcgcgccg gctggcagcg gataaggtgg gaccgattcc aaggttggct
     1861 aaaaagtctg atccttcggt cgtcaattcg cccgcatcgg catcgagaac gacatagcga
     1921 cgttcaatca tcgcgtctgt aagcgcgacg ccaagctcgc ctgccagatg atcgtagcag
     1981 gtccgcgccc ggcgcatatc gctatcgatt cttgacggcg gtcgaaacct cggcggaccc
     2041 gtcgctgcga ccgtcatcat gccctcaagc atctgcgcga ccagcggtgt agccagccgg
     2101 tagaaccggc gcggtccctg cttttccacc gacaaaagct cgcgcttgac gagcttggcg
     2161 agatgcgaac tcgccgtctg ggcggtgacc ccggccacgc tggccaattc gctggccgtg
     2221 agtgctcgtc cgtccattag tgatgacaga atgttggcgc gcccggggtc ccccaccagt
     2281 tgggcgacct ccgccagctg cggtgcgatg gtcggcatgg cccgctccac gttcaatcga
     2341 gcataatgca tccattggca tgacgttccc cgctgaggtt tcgatcccga tcgaagcgta
     2401 cggcgagata cttcgaccgc gatcgaatag aaactctgga aacatcgatg ccacgtcgcg
     2461 ttttccggtg accgaaaccg aacggatgga gaaaggctca atggaaaaaa ttccaatcga
     2521 gaaggccatg gagcattcga atatcgggac aagcgaaccg cagcccaccg acatgtttac
     2581 ccggcgatcg gcgatcaacg tatttggtac cgtcatcgcg atggtcaacc tctcatcatt
     2641 ggccatctca ccgtctcaag ctcaaaccga aggagcaatg accatgggtc aatacgcaat
     2701 gaacaagaaa accgtgagtg acgctgtcag cggcggcggc cttttggtag tcgcccaatg
     2761 ggaagccaaa gatgggcaag ccgataaggt tgccgacatc ctgcgtcgct ttctgcctga
     2821 ggcccaaagc gatcccggag cgaagctgtt cctgatcagc cggggacgag aaaatcccgc
     2881 acagtttctg ttttacgaac tgttccgcga cgaagcggct ttgaaagccc atcaggagag
     2941 cgggtatttc aaaaccttca tcgcgggcga agcactaccg cttctttcca agcgcgagcg
     3001 ggcgcaatac gcgttgctgt agccggttcg gacgaatcgc gactgcaaag tctcgaacaa
     3061 aacgaaaggg gccgggtcgg gagacaactc tcgcccgagc tctcttgttt gtcgttcaag
     3121 ccgcgggaac tggcgtaatc cgctggactc tttgtgagaa tcaaactgga ccgttcaggt
     3181 cacctcgatc gcgggtcgaa atcatgaaag gaaacagatc gcgggggatg tgagttgacg
     3241 gtatgtcgga ttgccgagcc acaaccggaa tattcccgag gcctggggtc acgacacaaa
     3301 tcaggacagg agttcgaatg gcggattttt cggtcgcgcc gcaggtcggc tcacgcgagc
     3361 agcattttcg cgttcccggt ggcgcgacga gaaatcggta gtccgatggc gtactgaagg
     3421 cgaacatcac gtcgttcaag gcaagggcag gttcgagatt tttcaggatt accatctgcg
     3481 cgtcggtgac gtgatggcag atacagatcc tccgaaggaa gcgccgattc aggaacggcg
     3541 ctttgatgaa accgaggttg atgcggcaaa agtcgtgacg tttacggaga tcacgccgat
     3601 caagggcgca gcgtttgctg cccagaccga tctattgccc gcccatctcg gtcttgacct
     3661 aaagggcggc cccattgtcg aacacgatgt gttcgccagc atctacaatc cgggaaagct
     3721 ggcgctgctg gtggcatgga aggacccgag agccgcaaac gcgtggtcgc caaggaagat
     3781 cgacggcatc gagcggctgc ggcaccgcaa gatccgcatc gtgcgcgact acggtcggtt
     3841 tgatcggcgg gaggccccgc agttctatcc cgacgtcaag ggcgccgaga ccaaacatcc
     3901 cgagcccgcg cattaaggga gggcctgggc gcaaccgccg aggcgtcgca gcccagcctt
     3961 ggctagaacc gcggcagttc aggaaaggcg agcttgccgc cgtggacgtg cacgcggccg
     4021 agttcggcgc agcgatgcag ggttggaaac accttgccgg gattgagcaa tccttgcgcg
     4081 tcgaacgcgc atttcagccg ctgct
//
LOCUS       DATNOI010000055         5761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_352223,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000055 DATNOI010000000
VERSION     DATNOI010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352223"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VN968_01850"
     CDS             <1..594
                     /locus_tag="VN968_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL28025.1"
                     /translation="ASGFVTLNLDYASRRKALESLAEDIHPAIELFASGIEGSIHFVG
                     HSMGGLLARAYLARYRPRRLGRVVMLGTPNSGSEIADRLKNVLPYRAWFGPAGQQLIT
                     TRDAALDAILPPVDYPVGVIAGNRSVYPISSAFLPKPHDGRVSVENTRLDGMMDHVVI
                     AVSHPSLVRNRLALQQTIAFLRDGRFTPPVVVTRAGG"
     gene            complement(652..1626)
                     /locus_tag="VN968_01855"
     CDS             complement(652..1626)
                     /locus_tag="VN968_01855"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HXL28026.1"
                     /translation="MPKAVVCRELGPPESLRLETFASASLAQGQARVAIHAAGINFPD
                     ILMAAGEYQLRPELPFTPGVEAAGEIVEVNGAAGVAVGDRVIVKMRHGAYADEAVVAP
                     SQLTPLPSTFDYAEGATFLAAHGTAYHGLIDRGQLRPGEVLLVHGAGGGVGLAAVEIG
                     KMLGATVIAAASSEEKLAVAQARGADHLVLYRREPFRDAVKRITDGRGADVVFDPVGG
                     EIFENSMRCIAWGARILVIGFTGGIGLARTNLLLIKGASVLGVRAGEAVRRNSALGEV
                     RLKALTEWAEAGKIRPNISHRLPLEDYAKAMRLLVDRKAIGRVALVMR"
     gene            complement(1626..2234)
                     /locus_tag="VN968_01860"
     CDS             complement(1626..2234)
                     /locus_tag="VN968_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HXL28027.1"
                     /translation="MIEFFFDCSSPWTYLAFHNIQPLAKEFGVEISWRPILVGGIFNT
                     INPSVYASREAPVPAKVNYQKKDMADWARSAGLEIKMRPTVFPVNSVKAMRGCIWLGR
                     EMIPFARAVFEIYWGEDRDISQGAVLAEACQRVGIDPQKFLAGIGEPAIKDQLKANTD
                     EVMARGGFGSPTIFVDRTDMYFGNDRMPLIREALQRRRASAA"
     gene            complement(2431..3132)
                     /locus_tag="VN968_01865"
     CDS             complement(2431..3132)
                     /locus_tag="VN968_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HXL28028.1"
                     /translation="MIDLHYAPTPNGWKISIMLEELGLPYTVVPVNIRAGEQFKPEFL
                     AISPNNRIPAIVDRLPADGGGPFSVFETGAILIYLAEKTGRFLPADMRGRSRVVQWVM
                     WQMGGLGPMLGQHGHFALYAPENIPYAIERYRDEAARLYRVLDTQLGKTGAYVAGDYS
                     IADIACFPWTMTHKAQGFTLDDYPSVKRWYAEVRARPQVQAGLAIGKFVKEPFDEEAR
                     KNMFGQKAKEMAGQK"
     gene            3267..4289
                     /locus_tag="VN968_01870"
     CDS             3267..4289
                     /locus_tag="VN968_01870"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HXL28029.1"
                     /translation="MLFPTTIAGSLPKPEWLAEPNTLWAPWKSSGPELSRAKRDATLL
                     AVKLQEDAGVDIVTEGEQARQHFVHGFLEKIEGIDFAHKVKMGIRKDRYKAMVPQVVA
                     PLRLTGRVHAIEARVARTHTTRKLKFTLPGPMTIIDTIADQYYGDRVRMAFAFAELLN
                     EEAKALQADGVDVIQFDEPAFNVYMDEVSEWGIKALERAAEGLTCATAVHICYGYGIK
                     ANTDWKETLGSEWRQYEEIFPAIAKSPIQQVAIECRNSRVPLELLGLLTGKIVQAGVI
                     DVASDTVETAEDVAKVIDDVSKFVPKSNIVATTNCGMAPMHRDIAEAKLMALGAGAKL
                     ARQRFG"
     gene            4289..4552
                     /locus_tag="VN968_01875"
     CDS             4289..4552
                     /locus_tag="VN968_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28030.1"
                     /translation="MFVSAKLAARIIATSLAAISLPARADDRTLLNACQALIDRAAQS
                     AQGAGAAGVKTDDAELQRCRQIVREWALRDARMTVDEHGRPLR"
     gene            complement(4553..5176)
                     /locus_tag="VN968_01880"
     CDS             complement(4553..5176)
                     /locus_tag="VN968_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28031.1"
                     /translation="MPLQNRVTPAGEIIATPHRGMFTGNRGIIHDPATRTLLARRWAN
                     KAWLTCVCEFKGRRREVMGGRSWTELFFLDEATAFAAGHRPCFFCRRDDANRFRTAWE
                     EGNGVRNVLADDMDTVLHRERLDGRAKRLHPLPMPIDRLPDGTMVQEGTESYLITQSR
                     TLLWSPGGYRGAGRIIKDAMLLTPPSTLRAMSAGYRPALHPSAEPAV"
     gene            complement(5227..5754)
                     /gene="gpt"
                     /locus_tag="VN968_01885"
     CDS             complement(5227..5754)
                     /gene="gpt"
                     /locus_tag="VN968_01885"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HXL28032.1"
                     /translation="MMAEHPELSEQERAGKAFPVSWDQFHRDCRALTWRLNEVGPFHA
                     VIAITRGGLVPAAIVARELGVRVIDTVCIASYDHTKQGDLKVLKGVSADTAKLGGGTG
                     NGLLIVDDLVDTGKTARLVRDMLPGAHFATVYAKPMGRPLVDTYITEVSQDTWIFFPW
                     DTALSFQPPIRDGAA"
BASE COUNT         1079 a         1872 c         1770 g         1040 t
ORIGIN      
        1 gcctccggat tcgtgacgct gaacctcgac tatgcgagcc gccgaaaggc tttggagtcg
       61 ctggccgagg acatccatcc ggccatcgaa cttttcgctt ccggcatcga aggctcgatc
      121 catttcgtcg gccattccat gggtggcctg ttggcgcgcg cctatctcgc aagataccgg
      181 ccaaggcggc tcggccgcgt ggtgatgctg gggacaccca acagcggcag cgagatcgcc
      241 gatcgcctga agaacgttct gccctaccgc gcctggttcg ggccggccgg acaacagctc
      301 atcacgacac gcgacgcggc cctcgatgcg atcctgccgc cggtcgatta tccggtcggc
      361 gtcatcgcgg gaaaccggtc ggtctacccg atatcgtcgg catttctccc gaagccgcat
      421 gacgggcggg tatcggttga gaataccagg ctcgacggca tgatggacca tgttgtgatc
      481 gccgtatccc atccgtcgct ggtgcgaaac aggttagctc tgcagcagac tattgcgttt
      541 cttcgggacg gtcggtttac gcccccggtt gtcgtcaccc gcgcaggcgg gtgatccagt
      601 aaacgctaac gtcagtgatt actggatgcc ccttggggca tgacgaggag tttaccgcat
      661 caccagcgcc acgcgcccga tggctttgcg atcgaccaat agccgcatcg cctttgcata
      721 atcttccagc ggcaatcggt gcgagatgtt cggccggatt ttgccggcct ccgcccactc
      781 cgtcagcgcc ttcaaccgca cttcgccgag cgccgaattt ctccgcaccg cctcgccggc
      841 gcggacgccc agcacgctgg cgcccttgat cagcaagaga ttggtgcggg cgagcccgat
      901 gccgccggta aaaccgatga cgagaatcct tgcgccccag gcgatgcagc gcatgctgtt
      961 ctcaaagatc tcgccgccga cgggatcgaa caccacatcg gcgccgcggc cgtcggtgat
     1021 gcgcttgacg gcgtcgcgga agggttcgcg gcgatagagc accagatgat cggcgccccg
     1081 cgcctgcgca accgcgagct tttcctccga ggaggccgcc gcgatcacgg tggcaccgag
     1141 catcttgccg atctcgaccg cggccagccc aacgccgccg ccggcgccgt gcaccagcag
     1201 cacttcaccg ggccgaagct ggccgcgatc gatcagcccg tgatacgcgg tgccatgggc
     1261 ggcgagaaac gtcgcgccct cggcatagtc gaaggtcgat ggcaacggcg tgagttgtga
     1321 cggcgccacc acggcctcgt cggcataggc gccgtgccgc atcttgacaa tcacccggtc
     1381 gccgacggcg acgccggcgg cgccattgac ctcgacgatc tcacccgcgg cttccacccc
     1441 gggggtgaac ggcaattccg gcctgagctg atactcgccg gccgccatca aaatatcggg
     1501 aaagttgatc ccggctgcat gaatggcgac gcgcgcctgc ccctgtgcca gcgacgcgga
     1561 ggcaaatgtt tccagacgca aactctcggg cggcccgagt tcgcggcaga cgacggcctt
     1621 cggcatcagg ccgcgctcgc tcttcgccgc tgcagcgcct cgcggatcag cggcatcctg
     1681 tcattgccga aatacatgtc ggtcctgtcg acaaagatgg tcggcgaccc aaacccgccg
     1741 cgcgccatca cttcatcggt attggccttg agctgatcct tgatcgccgg ctcgccgatg
     1801 cctgcgagaa acttttgggg atcgatgccg acccgttggc atgcttccgc cagcactgcg
     1861 ccttgcgaga tgtccctatc ctcgccccaa tagatctcga acaccgcacg cgcaaaaggg
     1921 atcatctccc ggcccagcca gatgcagccg cgcatcgcct tgacgctgtt gaccggaaat
     1981 accgtcggcc gcatcttgat ttcaaggcct gcggagcgcg cccagtccgc catgtctttc
     2041 ttctggtagt tcaccttggc cggcactggc gcctcccgcg aggcatagac gctcggattg
     2101 atggtgttga agatgccgcc aaccaggatc ggccgccacg aaatctcgac gccgaattcc
     2161 ttcgccagcg gctgaatgtt gtggaaggcg agataggtcc agggactgga gcagtcgaag
     2221 aagaactcga tcatgatggc gttttccctt gttattggtc tgtttgtagt caggtggatc
     2281 aacccaactc gtcattcccc gacgcggcaa aaataaaata atgtccaagt cggcaacagc
     2341 cgacttggat ggcgcaggcc ccgaatcgat aacacgacgg ggagtatgga ttccgggctc
     2401 gcgtttcgcg cgccccggaa tgacggattg ctatttttgt ccggccatct cctttgcttt
     2461 ctgcccgaac atattcttgc gcgcctcctc gtcgaacggc tctttcacga atttgccgat
     2521 cgccagcccc gcctgcacct gaggccgcgc gcggacctcc gcataccagc gttttacact
     2581 gggatagtcg tcgagcgtaa aaccctgcgc cttgtgggtc atggtccatg gaaagcaggc
     2641 aatatcggcg atcgaataat caccggcgac gtacgccccg gtcttgccca gctgggtatc
     2701 gagcacgcga taaagccggg cggcctcgtc gcggtagcgt tcgatcgcat aggggatgtt
     2761 ctccggcgca tagagcgcga aatggccgtg ctggccgagc atgggcccta acccgcccat
     2821 ctgccacatc acccattgca ccaccctgga gcggccgcgc atatccgcag gcaggaaacg
     2881 gccggtcttt tcggcgagat agatcaggat ggcgccggtc tcgaataccg aaaacggccc
     2941 gccgccgtcg gcaggcagcc ggtcgacgat cgccggaatc cgattgttcg ggctgatcgc
     3001 cagaaattcc ggcttgaatt gctcgccagc ccggatgttg acgggaacga ccgtataggg
     3061 aagccccaat tcctccagca tgatcgagat tttccagccg ttcggcgtcg gcgcataatg
     3121 aagatcgatc atggcatgat cctgcaagag acggccgcta tcctagcctg cggaataccc
     3181 ccgttccagt cgtgctggcg gtcttgtgga gcgcatggcg aacatggcac acaggcgcct
     3241 caatcagaac cgaccgggag gcccccatgc tgtttccaac cacgatcgca ggctccttgc
     3301 cgaagccgga atggctggcc gaacccaaca cgctgtgggc gccctggaag tcttcaggcc
     3361 ctgaactgtc gcgcgccaag cgcgacgcca ccctgctggc cgtcaagctg caggaggatg
     3421 ccggcgtcga catcgtcacc gagggcgagc aggcgcgcca gcatttcgtc cacggctttc
     3481 tggagaagat cgagggcatc gacttcgccc acaaggtcaa gatgggaatc cgcaaggatc
     3541 gctacaaggc gatggtaccg caggtggtgg cgccgctgcg gttgacgggc cgcgtccatg
     3601 ccattgaggc ccgcgtcgcc cgcacccaca ccacgcggaa attgaaattc accctgcccg
     3661 gcccgatgac catcatcgac accatcgcgg accagtatta cggcgaccgc gtcaggatgg
     3721 cgttcgcctt tgccgaactg ctcaacgagg aggccaaggc gttgcaggcc gacggcgtcg
     3781 acgtcatcca gttcgatgag cccgccttca acgtctacat ggacgaggtc tccgaatggg
     3841 gcatcaaggc gctggagcgt gctgcggaag ggctgacctg cgcaaccgcg gttcatatct
     3901 gctatggcta cggcatcaag gccaataccg actggaagga aacgctcggc agcgagtggc
     3961 ggcagtacga agaaatcttt ccggcgatcg ccaaaagccc gatccagcag gtcgcgatcg
     4021 aatgccgcaa ttcgcgggtg ccgctggaac tgctcggcct gctcacgggc aagatcgtgc
     4081 aggccggcgt gattgacgtc gccagcgata ccgtggaaac ggcagaggat gtcgccaagg
     4141 tgatcgacga cgtctccaaa ttcgtgccca agagcaatat cgtcgcgacc accaattgcg
     4201 gcatggcgcc gatgcatcgc gacatcgccg aggccaaact gatggcgctc ggtgccgggg
     4261 cgaagctggc gcggcagcgg ttcggctgat gtttgtctcc gcgaaactgg ccgcccggat
     4321 cattgccacg tcactggctg cgatatcgct gccggcgcgc gccgatgatc gcaccttgct
     4381 gaatgcctgt caggccttga tcgaccgcgc ggcgcaatca gcgcagggag cgggtgcggc
     4441 cggcgtcaaa acggacgatg cggaactgca gcgttgcagg cagatcgtcc gcgaatgggc
     4501 gttacgtgat gcgcggatga ccgtcgatga gcacggccgg ccgctgcgct agttagacgg
     4561 caggttcggc gctcggatgc agcgccggtc tatatcccgc tgacatagcg cgcagggtcg
     4621 aaggcggcgt cagcagcatc gcatccttga tgatgcgccc ggcgccgcga tatccgccgg
     4681 gcgaccacaa cagcgtccgg ctctgagtaa tcaggtaact ctccgtgcct tcttgcacca
     4741 tcgtaccatc gggcaaccga tcgatgggca ttggcagcgg atgcagccgt ttcgcccgac
     4801 catcgagccg ctcgcggtga agcacggtgt ccatgtcatc agcgagaaca tttctgacgc
     4861 cattgccctc ctcccacgca gtacgaaacc ggttggcgtc gtcgcggcgg cagaaaaagc
     4921 acggtcgatg ccccgcggcg aacgcggtgg cctcatcgag aaagaacagc tcggtccagc
     4981 tgcggccgcc catcacctcg cgccgccgtc ccttgaattc gcagacgcag gtaagccacg
     5041 ctttgttggc ccagcgccgc gccagcaggg tcctggttgc ggggtcgtgg atgatgccgc
     5101 gattgccggt aaacatgccg cggtgcgggg tcgcgatgat ttcgccggca ggagtgaccc
     5161 ggttttgcag cggcatgggc tgtctctgct ccctctctcc gcgcctgccg ggcgaggtaa
     5221 gaaggatcac gccgcgccgt cgcggatcgg cggctggaac gagagcgcgg tatcccacgg
     5281 aaagaagatc caggtgtcct gcgacacttc ggtaatataa gtatcgacca gcggtcgccc
     5341 catcggtttt gcgtacacgg ttgcgaaatg cgcgccggga agcatatcgc ggaccagccg
     5401 cgcggtcttg ccggtgtcga cgagatcatc gacgatcagc aacccattgc cggtgccgcc
     5461 gccgagtttc gcggtgtcgg ccgacacgcc tttcagaacc ttcaggtcgc cctgcttggt
     5521 atgatcgtag ctggcaatgc agacggtatc gatcacgcgc acgccgagtt cgcgcgcgac
     5581 gatcgccgcc ggcaccagcc cgccgcgggt gatggcgatg accgcatgaa acggcccgac
     5641 ctcgttcagc cgccaggtca gcgcccggca atccctgtga aactggtccc atgagacagg
     5701 aaaggccttg ccagcccgct cctgctcgct cagttccgga tgctccgcca tcatcatttc
     5761 c
//
LOCUS       DATNOI010000056         7777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1196250,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000056 DATNOI010000000
VERSION     DATNOI010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196250"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VN968_01890"
     CDS             <1..854
                     /locus_tag="VN968_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXL28033.1"
                     /translation="SGAGAELNVSAAAVSRMVHLLEERLGVALFERKANRLAMTSAGR
                     AYQNGLTPIFDALASLTAQVTAPSSVRVLTIGVGPTFAMKWLIPRLADFRKHQPDIDV
                     RITTGGAAAPFADDWNCGIKLGDGEWPGLVSEPLFAADLLPVCAPRLALQLNRPGDLK
                     GPSLLRVAHSPDDWPLWLKSSGIARMTARGPEFQFYGQALQAAVDGLGIAMGIRPYID
                     DDLAAGRLVAPFALSVPKGMRWYLVYRSFHTEQRDFAAFRRWIVRAAAGPAERPRRTG
                     KAQHHAG"
     gene            844..2070
                     /locus_tag="VN968_01895"
     CDS             844..2070
                     /locus_tag="VN968_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXL28034.1"
                     /translation="MPVEQVRTLIIGSGQAGLTMSHRLTQRGVAHLVLERHRIAERWR
                     SERWDGLRFQFPNWSVRLPDFPFPHTDPDGFATSREIVDFIGAYADFIAAPIRCGVAV
                     IGLRRRDGGPGLVAETSDGSIEADNVVVATGPYQSPVIPARLRDDINLFQVHASRYLN
                     PDQLPPGAVLVVGAGASGSQIAEELLRAGRRVYLSVGRHTRLPRRYRGRDLIWWLSAM
                     GIDQTAVEQRGPSRLLPLITGAYGGYTIDFRRFAADGVTLLGRAKAAHDGIIDLAPDL
                     AESLVNGDAMYASFLDREDAHIEHHALAIPQDPAARAVLSDPSCVTEPLRRLDLRAEG
                     IGAVIWATGYGVDFGWIDVPVLDARGEPVHHRGVTEVPGLYFLGLQWLSKMSSSFLSG
                     VGDDAADLADHIAARR"
     gene            complement(2175..5330)
                     /locus_tag="VN968_01900"
     CDS             complement(2175..5330)
                     /locus_tag="VN968_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXL28035.1"
                     /translation="MRLSTRLTVAMVALVLLATTAVGVLTYRNIAAFALPRALDRIDT
                     HAQLLGGELAASVRGARADVIGFRSSNAVIEIMTAHLNRGTDPAAAATEAEWRRRLGL
                     RFAAELISKPNYHVFRFIGADDGGRELVRVERSDPGGPPRIVPDSELRPKGHRDVFKQ
                     AIQLPPGGVYVSPVDFNIETPRIPVLRVATPVHAPDGRPFGIVIINVDMRPAFVRIRS
                     GATKGGRSYVVNDQGDYLVHPDPNREFGFEFAKPLHIQDDFPEFSQILATGDTAPRVI
                     QDRAGTRFGIGVVIERLADGPRVFVIQAVPYSTLMAATTAVRDSSLMGGFVAAFCAFL
                     LAIIVARSLTRPLVQMTKVVQGFSRDETVALPTGGGLEIGVLADAFAHMAAESRAKTA
                     ALNREIEERRRTFEREQLFIAAVESSNDAIVTETLDGVVTGWNPAAERMFGFTAREAI
                     GNSIDIIVPCDLRCETHATLDKIRGGEKIDYHETVRTGKDGRQIEVSLGISPIRSQSG
                     TIIGAAQVARDITARKKAQEALFESEQMARDVIANAFEAFIQTDEAGHILEWNPQAEA
                     IFGWSRPEALGRHPTSLLLSETLKPQFQTMSDRLVRNEQNAAAGARFETEAMRKDGRT
                     VKVEVSLKALRRRSGYVFNAFVRDLTQKIAAEEQLRQAQKMEAVGQLTGGIAHDFNNM
                     LTVITGTIEILAEGVADKPHLATIAKLISDAADRGAELTAHLLAFARKQPLQPRETDI
                     NRLIVDSAKLMRPTLGDHIEIVSMLADSAGAALVDPGQLSTVLLNLAINARDAMPSGG
                     KLTLETKNVTLDSMYAEANGDVQPGNYVMIAVSDTGIGIPEAIRDRVFDPFFSTKEVG
                     KGTGLGLSMVYGFVKQSGGHIKVYSEVGFGTTFKLYLPQAEAPPEQSAVEPLTPEMEG
                     GDETILVVEDDPLVRAYVNTQLQSLGYKTLSAANGVEALAIVDSGAAFDLLFTDVIMP
                     GRLNGRQLAAEMEQRHASLKVLFTSGYTENAIIHHGRLDSGVLLLAKPYRKLDLARLL
                     RVALTSAGNVPDASPPPEPRG"
     gene            5679..5927
                     /locus_tag="VN968_01905"
     CDS             5679..5927
                     /locus_tag="VN968_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28036.1"
                     /translation="MNYDGFQIESFEAGRGLWHARIRRADQEPVVIDGVLFSTLEVGF
                     AWSSPDAAVADAKTQIDHLNQRYATFGRTTQAEFPAAS"
     gene            6230..>7777
                     /locus_tag="VN968_01910"
     CDS             6230..>7777
                     /locus_tag="VN968_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HXL28037.1"
                     /translation="MAKNPDHPKKPVKTPKSKAPNSKASRPDVQPIGPALAELLNPAI
                     NRGDAGLGSGTGLQPPPDNSWDRRSGGEAAAHRARASTRGTSEDVAKRDASVTKRDDS
                     SSPPPRGEGSQAPPAQAPPVAYPPPQPSPARGEGVRKQGFDEAPQANYGTAATIPALD
                     PELARQLGFTTEEEDAAALARPPRNKMEALGVAATADALENLIREGRPEFKGEDGHVK
                     IWTPHRPPRPEKSEGGVRFVIKSEYEPKGDQPTAITELVEGIKRNDRTQVLLGVTGSG
                     KTYTMAKVIEATQRPAIILAPNKTLAAQLYGEFKSFFPDNAVEYFVSYYDYYQPEAYV
                     PRTDTYIEKDSSINEQIDRMRHSATRALLERDDVIIVASVSCIYGIGSVETYTAMTFA
                     LKKGERIDQRQLIADLVALQYKRTQADFTRGTFRVRGDVIDIFPAHYEDRAWRVNLFG
                     DVVENIEEFDPLTGHKQDELEFIKIYANSHYVTPRPTLVQAIKSIKSELKWRLDQLNN
                     QGRLLEAQ"
BASE COUNT         1420 a         2496 c         2411 g         1450 t
ORIGIN      
        1 tcagtggcgc gggcgcggag ctcaacgtgt cggcggcggc ggtcagccgg atggtgcatc
       61 tgttggagga gcggctcggc gtcgccctgt tcgagcgcaa ggccaaccgg ctggcgatga
      121 catcggccgg ccgcgcctat cagaacgggc tgacaccgat cttcgacgcg ctggccagcc
      181 tgaccgcgca ggtgacggcg ccgtccagcg tccgggtgct gaccatcggc gtcggcccga
      241 cttttgcgat gaaatggctg atcccgcggc tggcggattt tcgcaaacac cagcccgaca
      301 tcgacgtgcg gatcaccacc ggcggcgcgg cggcgccgtt cgccgacgac tggaactgcg
      361 gcatcaaact cggcgatggc gagtggccgg gtctggtctc tgaacccctg ttcgcggccg
      421 atctgctgcc ggtgtgcgcg ccgcgactgg cgctgcaatt gaaccgcccc ggcgatctga
      481 agggaccaag cctgttgcgc gttgcgcatt cgcctgacga ctggccgctg tggctcaagt
      541 catccggcat cgcgcgcatg acggcgcgcg ggccggagtt tcaattttac ggccaggcgc
      601 tgcaggccgc cgtcgacggg cttggcatcg cgatgggcat ccggccctac atcgacgacg
      661 atctcgccgc cggacggctg gtcgcgcctt ttgcgctgag cgtgccgaag ggcatgcgat
      721 ggtatctggt ctatcgcagc ttccataccg agcagcgcga tttcgccgca ttccggcgct
      781 ggatcgtgcg cgccgccgct ggacccgctg agcgtcccag gcgtaccggc aaggctcagc
      841 accatgccgg ttgaacaagt ccggacgctg atcatcggca gcggccaggc cgggctgacg
      901 atgagccacc ggctcacgca gcgcggcgtt gcccatctcg tgctcgagcg gcaccggatc
      961 gccgaacgct ggcggagcga gcgctgggat ggcctgcggt ttcagtttcc caactggtcg
     1021 gtgcggctgc ctgattttcc gtttcctcac accgatcccg atggctttgc gaccagccgc
     1081 gagatcgtgg atttcattgg cgcctatgct gatttcatcg ccgcgccgat ccgctgcggt
     1141 gtcgcagtaa tcgggttacg acgccgcgat ggcggccccg gccttgttgc cgaaacatct
     1201 gatggttcga tcgaagctga caatgtcgtc gtcgcgaccg gtccctacca gagccccgtc
     1261 ataccggccc ggttgcgcga tgacatcaac ctctttcagg tgcacgccag ccgctatcta
     1321 aatcccgacc agcttccacc cggcgcggtg ctggtggtcg gcgccggcgc ctcaggttca
     1381 caaattgccg aggaactgtt gcgcgcaggc cgccgcgtct acctctcggt cggccggcac
     1441 acccgcctgc cgcgccgata ccgcggccgc gatctgatct ggtggctgag cgccatgggc
     1501 atcgatcaga ccgcggtgga acagcgcggg ccctcgcgac tgctgccgct gatcaccggc
     1561 gcctatggcg gctacaccat cgacttccgc cgcttcgcgg cagacggcgt gacgctgctg
     1621 ggacgggcga aggcggcgca tgacggcatc atcgaccttg cgccggatct cgccgaaagc
     1681 ctggtcaatg gcgatgcgat gtacgcatcc ttccttgaca gggaggatgc gcatatcgag
     1741 caccacgcgc ttgccatacc gcaagatccc gccgcccgcg ccgtgttgtc cgatccgtcc
     1801 tgcgtcacgg aacccctccg gcggctcgat ctacgggccg aaggtatcgg cgcagtgatc
     1861 tgggccaccg gctacggcgt cgacttcggc tggatcgatg ttcccgtgct ggatgcgcgc
     1921 ggcgagccgg ttcaccaccg cggcgtcacc gaggtgcccg gcctgtattt cctcggcctg
     1981 caatggctgt ccaagatgag ttcgtcgttc ctgtccggcg tcggcgacga tgcggcggat
     2041 ctcgccgacc acatcgccgc gcgtcgttga ttgcggttgg gcttggcgcc gacggcgtgg
     2101 aaatgccggt gcagttgaaa gacagcagtc cgcgccctgc tccacacctt cgttcggaac
     2161 ggcgatctca aagctcagcc gcgcggttcg ggtggcgggc ttgcgtccgg aacatttccg
     2221 gcagaagtca atgcgacgcg aagcaagcgc gcgagatcca gcttgcgata tggcttggcg
     2281 agcagaagga cgccggaatc cagtctgcca tgatggatga tggcattttc ggtatagcct
     2341 gacgtaaaca acaccttcag cgacgcgtgt cgctgctcca tctctgcggc gagctgccgg
     2401 ccgttcaagc gcccgggcat gatgacatcc gtaaacagaa ggtcaaaggc tgcgccgctg
     2461 tcgacgatcg caagggcttc gacgccgttt gcggccgaca gagtcttgta accgaggctt
     2521 tggagctgcg tattgacata agcacgcacc agcggatcgt cctcgacgac caggatggtc
     2581 tcgtcgccgc cttccatctc gggggtcaac ggttccaccg cgctctgctc cggcggcgcc
     2641 tccgcctgcg gcagatagag cttgaatgtc gtcccaaagc ccacttcgct gtagaccttg
     2701 atgtgtccgc cggactgctt gacgaagccg tagaccatgc tcaaccccag accggttccc
     2761 ttgcccacct ccttggtcga aaagaacgga tcgaacactc tgtcgcggat cgcctccgga
     2821 attccgatgc cggtatcgct caccgcgatc atcacataat tccccggctg gacgtcgccg
     2881 ttggcttcgg catacatgct gtcgagcgtc acgttcttgg tttccagagt aagcttgccg
     2941 ccgctcggca tggcatcgcg ggcgttgatc gcaaggttga gaagcaccgt actgagctgc
     3001 cccggatcga ccaaagccgc gccggccgaa tccgccaaca tcgacacgat ttcgatgtgg
     3061 tcgcccaatg tcggccgcat cagctttgcc gagtcgacga tcaagcggtt gatgtcggtt
     3121 tcgcgcggct gcaggggttg cttgcgggca aaggcgagta gatgtgcggt aagttccgcg
     3181 ccgcggtcag ccgcatcgct aatcagcttg gcgatcgtag cgaggtgcgg cttgtcggcg
     3241 accccttcgg ccaggatttc aatggtgccg gtgatgacgg tgagcatatt gttgaagtca
     3301 tgggcgatgc cgccggtaag ctggccaacg gcttccatct tctgggcttg cctcagttgc
     3361 tcctccgccg cgatcttttg ggtcagatcc cttacgaatg cgttgaatac gtagccactg
     3421 cggcggcgaa gcgccttcaa ggacacttcg accttgacgg tacgcccatc ctttcgcatg
     3481 gcctcggtct caaagcgggc gccggcggcc gcgttctgct cgttccgcac cagccgatca
     3541 ctcatggtct gaaattgcgg cttcagcgtt tccgacagca gcagactggt cgggtgcctc
     3601 cccaacgcct ccggccgcga ccatccgaag atcgcctcgg cctggggatt ccattcgaga
     3661 atgtggccgg cttcgtcggt ctgaatgaac gcttcaaaag cattggcgat gacgtcgcgt
     3721 gccatctgct cgctttcaaa cagcgcttcc tgcgcttttt ttcgcgcggt aatatcgcgc
     3781 gcaacctggg cggcgccgat gatcgttccc gattgcgacc tgatcggcga gatgccgagg
     3841 gacacctcga tctgccgtcc atctttgccg gtgcgaacgg tttcgtggta atcgattttt
     3901 tcgccacctc gtattttgtc cagggtggcg tgagtctcgc agcgcagatc gcagggaacg
     3961 atgatatcga tgctgttgcc gatcgcctcc cgggcggtaa atccgaacat gcgttccgcg
     4021 gccggattcc agccggtgac gacgccatcg agggtttcgg tcacgattgc atcgttggac
     4081 gattccacgg ctgcgatgaa gagctgctcg cgctcgaagg tgcgccggcg ctcttcaatc
     4141 tcccgattga gtgctgccgt cttggcgcgc gactcggccg ccatgtgtgc aaacgcatcg
     4201 gccagcacgc cgatctcaag gcctcccccg gttggcagtg cgaccgtttc atcgcgggaa
     4261 aacccctgga cgaccttggt catctgaaca agcggccgcg tcagcgagcg ggcgacgatg
     4321 atggccagca ggaatgcgca aaacgccgca acgaatccgc ccatcaagct ggagtcgcga
     4381 accgcggtgg ttgccgccat gagtgtcgag tacggcaccg cctggatcac gaagacccgg
     4441 ggaccgtccg ccagacgctc gatcaccacg ccgatcccga agcgcgtgcc ggctcgatcc
     4501 tgaatcacgc gcggggccgt atcgccggtc gcgagtatct gggagaattc cggaaagtcg
     4561 tcctggatgt gaaggggctt ggcaaattcg aacccgaact cgcgattggg gtcgggatgg
     4621 acgagataat caccctggtc attgacgaca tagcttcgac cgcctttggt ggcgcccgag
     4681 cggatccgga caaatgccgg ccgcatatcg acgttgatga tcacgatgcc aaacggccgc
     4741 ccgtccggcg cgtgcaccgg cgtcgcgacg cgcagcaccg ggatacgcgg cgtttcgatg
     4801 ttgaaatcga ccggcgagac atagaccccg ccgggcggta actggatagc ttgcttaaag
     4861 acatcgcgat gccccttggg ccgcaactcg ctgtccggaa cgatgcgcgg cgggccgcct
     4921 ggatccgaac gctcgacccg gacgagttcg cgcccgccgt cgtcggcccc gatgaagcgg
     4981 aacacgtgat aatttggctt ggagatgagt tccgcagcga agcgcaatcc caggcgtctt
     5041 cgccactccg cctcggtggc ggcggccgct ggatcagttc ctcgattgag atgcgccgtc
     5101 atgatttcga tgacggcgtt ggaagagcga aagccaatca catcggcgcg cgcgccgcgg
     5161 actgaagctg caagctcgcc gccgagcagt tgagcatgcg tatcgatccg gtccaatgcc
     5221 cgaggcagag caaacgctgc gatattgcga tacgtcagca ccccgaccgc cgtcgtcgcc
     5281 agcagcacca acgcgaccat cgcaacggtc aaccgagtcg acaacctcat gatcgccctc
     5341 tgggcggcgc cttcctcgac gtcggcgcca agttgccccg tggtctatct cgaatcaccc
     5401 gaccgcggta atctttctgg cacggcggta aatgcgcgaa tcgattgatg actcgattaa
     5461 cgagttgttc tgatgggcgt tggctgatct cgccgttcat attaccgcgc gcacttgatc
     5521 cggattcgga ctcatgcgtc atggctggcg caactgtcct gtttgcaggc ccgaaaaata
     5581 agcccggcat gtgaatgagt tcacatcggt tttggtgggc tcatggttct ttcggctccg
     5641 gaaaatactc tctgcgaaaa gaacctggag tcacgacgat gaactatgac ggctttcaaa
     5701 ttgagtcctt cgaggccgga agaggtttgt ggcatgccag gatcaggcgg gcggatcaag
     5761 aacctgtcgt catcgacggc gttttgtttt ccacgctcga agtcggcttc gcgtggtcga
     5821 gtcccgatgc ggcggtcgcg gatgcgaaga ctcaaatcga ccacctcaac cagcgatacg
     5881 cgacgttcgg gcgaacgacg caggcggaat tccctgctgc ttcctgacaa tagcccgcat
     5941 tccatgtgca aatctgaagc cgaaccgact ccgttgccgt cagcgatggt acgctccaat
     6001 tgtcccctct gcgacacccg cctgatggta caccgcatta tccccggacc gtcagagctt
     6061 gaatattgga cgttgcgatg cacgaaatgc ggccatatgc atcaggatgc ggtccgttcg
     6121 ggcctcagag actgatgaca ccggccattc tttgatgccg ccgggcagac gctaattcgc
     6181 ggtcggcttt gcccttttat cgccgccgga ctcatcccat attcggctca tggcgaagaa
     6241 tccggaccat ccgaaaaagc ccgtcaaaac cccgaaatcg aaggcgccaa attctaaggc
     6301 gtcgaggccc gacgtgcagc cgatcgggcc ggcgctggcg gaactgctca atcccgcgat
     6361 caatcgcggc gatgccgggc tcggctccgg aaccggtttg cagccaccgc cggataattc
     6421 ctgggatcgc cgctccggcg gcgaggccgc agcacatcgc gcgcgggcgt cgacccgtgg
     6481 aaccagcgag gacgtggcca agcgcgatgc gtcagtcacg aagcgcgacg actcttcctc
     6541 ccctccccct cgcggcgagg ggtcacaggc gccacccgcg caggcgccac ccgtggctta
     6601 cccccctccc caaccctccc cggcaagggg ggagggagta aggaagcaag gcttcgacga
     6661 agccccgcag gccaattacg gcacagccgc gacgattccg gcactcgatc cggaactggc
     6721 aaggcaactc ggtttcacca ccgaggagga agacgccgcg gcgctggccc ggccgccgcg
     6781 caacaagatg gaggcgctgg gcgtcgccgc taccgcggat gcgctggaaa acctgatccg
     6841 cgaaggacgg cccgaattca agggcgaaga cggccacgtg aaaatctgga caccgcatcg
     6901 tccgccgcgc ccggagaaat ccgaaggcgg cgtccgcttc gtgatcaaat ccgaatacga
     6961 gccgaagggc gaccagccga ccgcgatcac ggagcttgtc gagggcatca agcgcaacga
     7021 ccgcacccag gtgcttctcg gcgtcaccgg ctccggcaag acctacacca tggccaaggt
     7081 gatcgaggcc acgcagcgcc ccgcgattat tctggcgccg aacaagacgc tggcggccca
     7141 gctctatggc gagttcaaga gcttcttccc cgacaacgcc gtggaatatt ttgtctcgta
     7201 ttacgactac taccagcccg aggcctatgt gccgcggacc gacacctata tcgagaagga
     7261 ctcctcgatc aacgagcaga tcgaccgcat gcgccactcg gcgacgcggg cgcttctgga
     7321 gcgcgacgac gtcatcatcg tggcttcggt atcgtgcatc tacggtatcg gctcggtcga
     7381 gacctacacc gcgatgacgt ttgcgctgaa aaagggcgag cgcatcgacc agcggcagct
     7441 gatcgccgat ctcgtcgcgc tgcagtacaa gcggacgcaa gcggatttca cccgcggcac
     7501 gtttcgcgtc cgcggcgacg tcatcgatat ctttccggcg cactatgaag accgcgcctg
     7561 gcgcgtgaac ctgttcggcg acgtcgtcga aaatatcgag gagttcgatc cgctcacagg
     7621 ccacaagcag gacgaactcg aattcatcaa gatctacgcc aactcgcact atgtgacgcc
     7681 gcggccgacg ctggtgcagg cgatcaagtc gatcaagagc gagttgaagt ggcggctgga
     7741 tcagctcaac aatcagggcc gcctgctgga agcgcag
//
LOCUS       DATNOI010000057        14329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2280451,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000057 DATNOI010000000
VERSION     DATNOI010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 14329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14329
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2280451"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..2249)
                     /locus_tag="VN968_01915"
     CDS             complement(270..2249)
                     /locus_tag="VN968_01915"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HXL28038.1"
                     /translation="MKQFLIFSAAVATALLGLGDAALADCNAIDTDQTCTNSTLLSGF
                     RGLQDNGTTLTVTNTASGTISGSQAGIFANNNANVTNSGTISGGFSGVEATTANVTNS
                     GTISGGTFGIDAGTATVFNSGTIVGHNGDGVFADGTATVTNSGTISGSFNGVEATTAN
                     VINSGTISASGAGGAAIFALDGTANITNSGTIKGETGIFANGGATLINSGSIIGTGGT
                     AIDFSNSASDSLTFLPGSRIIGNILLGANDTVNIRSGRDVAWLLTFGNANGVAEPVGT
                     GASVNVSGGAPFVISGSQVATLDPTAFGLADRSLMNFTGGISSLVESRFGGMAPVGSS
                     SDVSSFAPAVNGPTATEAFNGIPSLAIAYAPETPGAFKAPPLAGVGTTTVWTGAFGGL
                     RHQQADGPMLAATDSALGAALGIDWLVASDLRLGGFVGGGAGRLAVDLNSQTVKTDYL
                     FGGAYGRYDWASQFADFMLYGGQARNTSTRTVANNLAPSGLETATASYDGFFISPDVK
                     YGWRLPVGGAYTLTPTARVRYVAGFFDGFSETGSAQNLSVGSRTLQDFEERLEVDLSG
                     ITAFGSTGTLKSGVHVGAIGVERVGDTTVNTVLLGQNLAFVTPGKVNAFGGLAGASFD
                     FATHAHISLFGALEGIWMNDGSTTVTAKAGLRGAF"
     gene            2809..3324
                     /locus_tag="VN968_01920"
                     /pseudo
     CDS             2809..3324
                     /locus_tag="VN968_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616016.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(3395..3550)
                     /locus_tag="VN968_01925"
     CDS             complement(3395..3550)
                     /locus_tag="VN968_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HXL28039.1"
                     /translation="MSNRRIDPKSADAIELNDEQLKSVTGGRKGGDDRLPYFAIEMKH
                     VTVTSWT"
     gene            complement(3755..5365)
                     /locus_tag="VN968_01930"
     CDS             complement(3755..5365)
                     /locus_tag="VN968_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXL28040.1"
                     /translation="MKQSDETWWRHGIFYQAYPRSFQDSNADGVGDIAGLIERLPYLL
                     ELGIDAVWLSPIFPSPMADFGYDISDYTGVDPLFGSMADFDALIGAAHANGLKLILDL
                     VPNHSSDRHPWFLESRSSRDDAKRDWYIWREPAADGGPPNNWMSEFGGSAWAYDAATR
                     QYYYHAFLAQQPDLNWRNPEVRQAIHDVMRFWLRKGADGFRVDVIWHLIKDEQFRDNP
                     PNPDFREGQPPHWKVLPLRTTDQPEVHDVIAEMRRVIDEFDARVLIGEIYLPFERLVT
                     YYGKDLAGAHLPFNFALLSAPWHARAIEKIIADYEAALPAGAWPNWVLGNHDRPRVAS
                     RVGPAQARVAAMLLLTLRGTPTLYYGDEIGMHQVAIAPDQVRDPFEKNVPGIGMGRDG
                     CRTPMQWNATPYAGFSTVAPWLPLPDDFAQDNVVNLDADQRSILSLYKALIDLRKKMP
                     PLVSGAYVPIAAQGDLLIYRRQGDADAVVIALNMGAEPVSIASEMFRLDGEILLSSFL
                     DRQGEKIQGVLDLRGNEGVIIGGHRDKS"
     gene            5526..6206
                     /locus_tag="VN968_01935"
     CDS             5526..6206
                     /locus_tag="VN968_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL28041.1"
                     /translation="MAQQTETETHTKAKAGAIIVPVTLFEQNCTVIWCEATKKAVVID
                     PGGDVPKILAAIKQTHVSVEKIWLTHGHIDHVGGAAELRDTLKVPIEGPHIADKHLLD
                     NAVTSGARFGMTGVRDFAPDRWLDEGDQVSIGALTFDVLHCPGHSPGSVVFFNKEMRF
                     AHVGDVLFNGSVGRTDLPGGSHATLIKSITDKLLPLGDDVGFICGHGAGSSIGQERLT
                     NPFLTGEM"
     gene            complement(6270..6647)
                     /locus_tag="VN968_01940"
     CDS             complement(6270..6647)
                     /locus_tag="VN968_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL28042.1"
                     /translation="MAKTKSALTCGCPVAAFQKMISGKYKLRIVWDLQDGPKRYGEIR
                     TGLLRGSDGSSEIAPRVLSRELKALTEQGLIDRRDYGVVPPKVEYRLTRKGKSFVPVI
                     ASIRDWGTRHLAQVNTVEMAAAE"
     gene            6779..7660
                     /locus_tag="VN968_01945"
     CDS             6779..7660
                     /locus_tag="VN968_01945"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL28043.1"
                     /translation="MILVTGATGLNGMELLRRLSARGVAVRALVRDPAKAEAIAALPH
                     VEIAQGDMARPETLAAALHEINRAMLISSSAPDMFDVQSNFIDAARKAGVRHVVKLSG
                     IMPGLHSAFRFARMHGEIENRLEASGMAYTHLRAGEFMPAYFRQVPPIVAKGAMFLPM
                     EDARIASIDVGDIAEIAARVLTGSGHEGKAYPLTGPEALTMTEVAAKLSTATGKPVRY
                     VNVPPEDARQAQLAAGMPPYLADALFELFAERRNGKEGKVWPDAATLLGRPPTSFDEF
                     ARRNAALFRGEAPPPKV"
     gene            7687..7989
                     /locus_tag="VN968_01950"
     CDS             7687..7989
                     /locus_tag="VN968_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28044.1"
                     /translation="MKHFMIKYQFANGTTEAWHREIEIFISALDHDPDLKGKISYRCM
                     KNRDDSSYIHLAAAVDEQAIKTLQARDFFKLYTEKTRQVAGGEVVVTPIELIAETA"
     gene            complement(8240..10729)
                     /locus_tag="VN968_01955"
     CDS             complement(8240..10729)
                     /locus_tag="VN968_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXL28045.1"
                     /translation="MLKTNGPTHADFLAGGGEMGALTRAYDWSASPLGAPEAWPQSLR
                     TALRILLNTNHPMFIWWGEELIQFYNDAYRQTMGPERHPSALGQRGRECWAEVWDIIG
                     PQIEQVMSGGGATWHENQLVPVTRHGRLEQVYWTYGYSPIDEDDRVGGVLVVCRDVTK
                     EYLAAAALREREAELARVQQIGRIGGLEVDLRTGFRNRRSPEYLLIHGLPPEAADESH
                     EDWVRRIHPEDRTATEKKFRDAVAGKVRDYNAQYRIIRPSDGETRWISVRSTIERDAQ
                     GRAIRLVGAHTDVTEQVMAEQALRQSEERYRKLADQLAGLSATLAQRVEEKTRERDRI
                     WNVSQDLLVVADQGGIWRTVNPAWTRTLGWSEAELLDRSSDWLEHPDDGGLTRAQFKK
                     LGTSGTTVRFESRFRHKDGSYRWLSWTGVSDEDATYAVARDVTAEKAAAERLKAAEEA
                     LLHSQKMEAVGQLTGGIAHDFNNLLTGIVGSLDLLQTRLDQGRTDNATRYINAAMTSA
                     NRAAALTHRLLAFARRQPLIPKSVDANQLVVSLEDLLRRTIGETIDLDIVAANDLWLT
                     LCDPNQLESALLNLAINARDAMPGGGKLTISTANVRLDGVTADSPALLPGDYVCIGVT
                     DTGTGMSAEVAARAFDPFFTTKPIGQGTGLGLSMIYGFARQSNGHATIDSRLGQGTSV
                     KLFLPRHHGDIASQHASAIGAAEHAVTGETVLVVEDEAVVRAVILEMLADQGYRTLEA
                     VDGPSGLRILRNPERVDLLVTDVGLPGMNGRQLADQARETRPDLKILFITGYAESVAM
                     SDSFLQPGMEMITKPFDLEILSRRIRAMISG"
     gene            complement(10938..12215)
                     /locus_tag="VN968_01960"
     CDS             complement(10938..12215)
                     /locus_tag="VN968_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HXL28046.1"
                     /translation="MSLANNVEFLRRLPKLQGLNGFGDFARNLRPGVRSPLVEITGFG
                     SNPGALKMFSFVPDSLQPAPALVVVLHGCGQTAAGYDLGAGWSTLAKHYGFALLMPEQ
                     ASSNNTQGCFNWFNPEDTARDSGEACSIRQMIARMVGDHKIDQHRIFVTGLSAGGAMT
                     SVMLATYPEIFAGGAIIAGLPFGVATNVREALSGMFVSPSRPASELGDLVRNASNHRG
                     PWPKLSVWHGSADRTVNPANANEIVKQWLDVHQLPAAPMSEADVDGYPRQVWWNADGE
                     TIVESYTITDMAHGTPLGVGDNDERYGAQGAFLIEAGISSSYHIANFFGLTEWIRQPE
                     VAPAEAAKETTKETVKETVKETAKPAVKPTPAVSPTPVKVPDLTAVLWPRTTLNRRPE
                     SSRRPEPPRAPRRRGIDVGAVITRALTAAGLMK"
     gene            complement(12357..13775)
                     /locus_tag="VN968_01965"
     CDS             complement(12357..13775)
                     /locus_tag="VN968_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL28047.1"
                     /translation="MHQIVSAPVDSSRRWWVLAIVVAAQFMFGVDAFIVNVAIPTIAV
                     ELHASPAQIEAVIAIYLIAYATLVVTGGRLGDIHGTKNVFLAGVFGFSVTSLWCGLAQ
                     SGPELIAARLAQGATAALMVPQVLATLHLLFSDTSRSRAFGIYGIVLGLAGAAGFLLG
                     GVLVTLDLAGLGWRAVFFVNVPFGLIIILAALRIMPSVPRRAGTRLDVPGAFVLFAGL
                     LCLIGPLLFGHDVHWAPGIWLVMAAGVAIVAAFLRLERAVASRDGMPLIDLALLSDAA
                     FMRGLFAAFFFFFANLSFYLVMTMFMQRGLRIPPLQAGSVFLPLALAFVIASRHSGAR
                     ARHRGTRVLIEGCAVQIVGLAVLVLTVATDVTPAAPLLASVLAIFGYGQGLVMAPLSS
                     AVLSTVKPASAGSGAGMYGTTAQIANAAGVAAIGGAFFAIEAARSARLALFAASALFA
                     VSIVACAAFLSWMRHALPPDTD"
     gene            13942..>14329
                     /locus_tag="VN968_01970"
     CDS             13942..>14329
                     /locus_tag="VN968_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL28048.1"
                     /translation="MYDYDMVVIGFGPSGRRAAVQFAKLGKSVLVVEKGRRVGGVSVH
                     TGTIPSKTLRETVLNLSGWRERGFYGRSYRVKQDIGATDLIARLQKTLDHEVEVLEHQ
                     FSRNMVKTASGEGRFLGPNKIEINAGG"
BASE COUNT         2742 a         4565 c         4437 g         2585 t
ORIGIN      
        1 gtcaatttca aatccgtccg accttgcagt tttccgtgcc ctgagcgatt ctcccgcaac
       61 aaccgcacac ttcatttttg cgccagaaag tagctagaaa aggtagccaa tgcctgcctc
      121 gaacgccggt ggttatgggt cccggcgttc gccgggacga cgttgttgta acgaacggga
      181 accccgcaaa gccctcgcca cttatccgcc tgccgccaac aaccctctcc ccgcgaagtg
      241 cggagcgagc gagaagccgg cccgcgccgt cagaacgcgc cgcgcaggcc ggccttggcg
      301 gtgacggtgg tgctgccgtc gttcatccag atgccttcga gcgcgccgaa caggctgata
      361 tgcgcgtggg tcgcgaagtc gaagctggcg ccggcgaggc cgccgaaggc attgactttg
      421 ccgggcgtca cgaaggcgag gttctggccc aacaagacgg tgttgaccgt ggtgtcgcca
      481 acccgctcga caccaatggc gccgacatgc acgccggatt tcaacgtgcc ggtgctgccg
      541 aacgccgtaa tcccggagag atcgacctcg agccgctcct cgaaatcctg gagggtgcgg
      601 ctgccgacgg aaagattctg cgccgagccg gtctcactaa aaccgtcgaa gaagccggcg
      661 acatagcgca cgcgcgcggt cggggtcagc gtgtaggcgc caccgaccgg caggcgccag
      721 ccatacttga cgtcggggct gatgaagaag ccgtcatagc tggcggtcgc ggtctcaagc
      781 ccactgggcg cgaggttgtt ggcgacggtg cgggtgctgg tgttgcgggc ctggccgcca
      841 tagagcatga aatcggcgaa ctgcgaggcc cagtcgtacc ggccgtaagc gccgccgaac
      901 aggtagtcgg tcttgaccgt ctgcgagttg agatcgaccg cgagccgccc ggcgccgccg
      961 ccgacgaaac caccgagccg caggtcagat gcgaccagcc agtcgatgcc gagcgccgcg
     1021 ccaagcgcgc tgtcggtcgc cgccagcatc ggaccatcgg cctgttgatg gcgcagcccg
     1081 ccgaacgcgc cggtccacac cgtggtggtg ccgacgcccg ccaacggcgg tgccttgaag
     1141 gcgccaggtg tttccggtgc ataggcgatg gcgagcgacg ggattccgtt gaacgcctcc
     1201 gtcgcagtgg ggccgttcac ggccggtgcg aagctgctca cgtctgaact cgagccgacc
     1261 ggagccatgc cgccgaagcg gctctcgacc agcgacgaga tcccgccggt gaaattcatc
     1321 agcgagcggt cggcgaggcc gaacgcggtc gggtcgaggg tggcgacctg gctgccgctg
     1381 atcacgaacg gcgcgccgcc gctgacgttg accgaggcgc cggtgccgac aggctcggcg
     1441 actccattgg cgttgccgaa cgtcagcagc cacgccacgt cgcgtccgct acggatattc
     1501 acggtgtcgt tggcgccgag caggatgttt ccgatgatgc gcgagccggg aaggaaggtc
     1561 agcgaatccg atgcggaatt gctgaagtcg atcgcggtgc cgccggtgcc gatgatgctg
     1621 ccggaattga tgagggtggc gccgccattg gcaaatatgc ctgtttcccc cttgatcgtc
     1681 ccggaattgg tgatgttggc ggtgccatcg agtgcaaata tggctgcgcc acccgccccg
     1741 gacgccgaga tggttccgga gttgatgaca ttggccgtgg tggcttcaac gccgttgaag
     1801 ctgcctgaga tggtccccga attcgtgacg gtggcggtac cgtcggcgaa aacgccgtct
     1861 ccattgtggc caacgatggt cccagaattg aagacggtgg cggtgccggc atcaataccg
     1921 aacgtgcccc ctgagatggt gccggagttg gtgacgttgg cggtcgtcgc ctcaacgccg
     1981 ctgaagccgc cggagatggt cccggaatta gtgacatttg cattattgtt agcaaaaatg
     2041 cccgcttggc ttcctgagat ggtgccggac gcggtgttgg tcaccgtgag cgtcgtgcca
     2101 ttgtcctgaa gtccgcggaa tcccgacagg agcgtcgaat tcgtgcaggt ctgatcggtg
     2161 tcgatcgcgt tgcaatccgc cagtgccgca tcgccgagtc ccagcagcgc cgtcgcaact
     2221 gctgcggaaa atatcaaaaa ctgcttcatt ccaattcccc gacgaggcct gattccagtc
     2281 gcgcgaatcc ggatcgtttt ggttcgcgct ttgcggggtc acgctacacc ggcttcgcgc
     2341 cggagcgttt gacaccggcc gaactagatc aggcgctgcg ttggacggga ccgaactgca
     2401 gcaccatttc ccgacgacca ttgcttttgc gcaacagctc gcgccgccga tcacggcgac
     2461 agatgcgggc gtggtctcgc acagcccgtg gggcggatta gccgaaggcg taatccgccg
     2521 cctacggggc tgccgggccg taggacggat tgtaatccgc ccttcaccga cgggttacgc
     2581 cccatcgggc cgcgcgttgc gcggacccgt tgggctacaa cccgccctgc gcactgcgct
     2641 tgattagagc aggtagccaa tgcctgcctt aaactcgaac gccggtggtt atgggtcccg
     2701 gcgttcgccg ggacgacgtt gttgtaacga acgggaaccc cgcaaagccc tcgtcactta
     2761 tccctcggcc gctaacacgg gaggcggacc ggagagggct gcctcggcat gatcgacgac
     2821 ctctggtaca agaacggcgt gttctattgt ctctccgtcg gcacctatat ggacgccaat
     2881 ggcgacggca tcggcgactt caaggggctg ttgcggcggc tggattatct gcacgggctc
     2941 ggcgtcaccg cgatctggct gatgccgttc cagccgtcac ccggcaagga cgacggctac
     3001 gatatcgccg actattatgg cgtcgatccg cgctacggca cgctcggcga ttttgtcgag
     3061 ttcacccatg gctgccggca acgcggcatc cgcgtcatca tcgacctcgt cgtcaaccac
     3121 acctcggacg cgcatccgtg gtttcgccac gcacgaagct cgcccgattc ggaataccgc
     3181 gactggtatg tctgggccga caagaagccg gccagtgccg gcaagggcat ggtgtttccc
     3241 ggcgtgcaaa aatccacatg gacgcatgat aaggaagcca aagcctggtg cttccaccga
     3301 ttctatgact tccagcctga tctgaagcgc tgaaatctta ccctttgcta ttaagcgagc
     3361 cgaagcaaac tgcgctgtcg aagctaatct tccgtcaggt ccaactcgtt acggtcacgt
     3421 gcttcatttc aattgcgaag taaggaagac ggtcatcacc gcctttgcga ccgcccgtaa
     3481 cggatttgag ctgctcgtcg ttcagttcga tggcgtcggc gctctttggg tcgattctgc
     3541 gattgctcat ggtcttctcc tctgaaacac cacgacccta ctgcgttcac cattcccgag
     3601 actgtgacgc ctgtcacgcg gcgagacgat ggcggaactg gctgcggtgt atggcgtcag
     3661 cgagccaacg atctggcggg cgctccaatg atccaatcaa agagcgcaag ggccacttgt
     3721 gatttttctc acaggctcct tcgccgggac gacgttagct cttgtccctg tgtccgccga
     3781 taatcacacc ctcattgccg cgcaaatcca gcacgccctg gattttttcg ccttgccggt
     3841 cgaggaagct cgaaagcaga atttcgccgt caaggcgaaa catctccgac gcgatcgaga
     3901 ccggctctgc ccccatgttc agcgcgatca ctaccgcgtc tgcatcgccc tgcctgcggt
     3961 agatcaatag atcgccctgt gcggcaatcg gaacgtaggc gccggacacg agtggcggca
     4021 ttttctttct caagtcgatc agcgccttgt agaggctgag gatcgatcgc tggtccgcgt
     4081 cgagattgac gacgttgtcc tgcgcaaaat catccggcag cggcagccac ggcgccaccg
     4141 tcgagaagcc ggcataaggt gttgcgttcc attgcatcgg cgtccggcag ccgtcgcgcc
     4201 ccatcccgat gccgggaacg ttcttctcaa agggatcgcg cacctgatcg ggcgcaatcg
     4261 ccacctgatg catgccgatc tcgtcgccgt aatagagcgt cggcgttccc cgcaacgtca
     4321 atagcagcat cgcggcgacg cgtgcctgcg ccggcccgac ccggctcgcc acccgcggac
     4381 ggtcgtgatt gcccagcacc cagttcggcc acgcccctgc cgggagtgcg gcttcgtagt
     4441 cggcgatgat tttctcgatg gcccgcgcgt gccatggcgc cgacagcaac gcaaaattga
     4501 acggcagatg cgcgccggcg agatccttgc cgtaataggt aacgaggcgc tcgaacggaa
     4561 ggtagatttc accgatcaaa acgcgggcat cgaattcatc gatcacccgg cgcatctcgg
     4621 cgatgacatc atgaacctcg ggctgatcgg tggtgcgcag cggcagtacc ttccagtgcg
     4681 gaggttggcc ctcgcgaaaa tccggattgg gcggattgtc gcgaaattgt tcgtccttga
     4741 tcaggtgcca gatcacatcg acccgaaagc cgtcggcgcc tttgcggagc cagaagcgca
     4801 tcacgtcgtg gatagcctgg cgcacttccg ggttgcgcca gttcagatcc ggctgctggg
     4861 cgaggaaggc gtgataataa tactgccgcg tcgcggcgtc atacgcccag gcgctgccgc
     4921 cgaattccga catccagttg ttcggcggcc cgccgtccgc ggccggctcg cgccagatat
     4981 accagtcgcg cttcgcatca tcgcgcgagc ttcggctttc aagaaaccag ggatgccggt
     5041 cggagctatg gttcggaacc agatcgagga taagtttgag gccgtttgca tgcgcggcgc
     5101 cgatgagcgc gtcgaaatcg gccatggagc cgaacagcgg atcgacgccg gtgtaatccg
     5161 aaatgtcgta gccgaaatcg gccatcggcg agggaaagat aggcgacagc cagaccgcgt
     5221 caattccaag ctccagcaaa tagggtagcc gctcgataag gccagcgata tcgccgaccc
     5281 cgtcggcatt ggaatcctga aacgagcgtg gataggcctg gtagaaaatg ccgtgccgcc
     5341 accaggtttc gtcgctctgc ttcattctgg aaatcattct ttgcaatcgt actgcgcgag
     5401 gcaacgcctg gaaccgctag ccggttcagc ttgaaccagc aagagatgac acgggagcgt
     5461 cagccgccca ctttttcagc ccaatttccc cttggcgagc cgcaaaaaat cggcattgtg
     5521 ctgacatggc acaacaaacc gagaccgaaa cccacaccaa agccaaggcc ggcgcgatca
     5581 tcgttccggt tacgctgttt gagcagaact gcacggtgat ctggtgcgaa gccaccaaga
     5641 aggccgtggt gatcgacccc ggcggcgacg tcccgaaaat tctcgccgcg atcaagcaga
     5701 cccatgtgtc ggtcgaaaag atctggctga cccatggcca tatcgaccat gtcggcggcg
     5761 ccgccgaact gcgcgatacg ctgaaagtgc cgatcgaagg cccgcacatc gccgataaac
     5821 atctgctcga caacgccgtc accagcggcg cgcggttcgg catgaccggc gtgcgcgatt
     5881 tcgcgccgga tcgctggctc gacgaaggcg atcaggtttc gatcggcgcg ttgaccttcg
     5941 acgtcctgca ctgccccggt cattcgcccg gcagcgtggt gttcttcaac aaggaaatgc
     6001 gctttgccca tgtcggcgac gtgttgttca acggttcggt cggccgcacc gacctccccg
     6061 gcggcagcca cgccacgctg atcaagtcga ttaccgacaa actgctgccg ctcggcgacg
     6121 atgtcggttt catctgcggc cacggcgccg gctccagcat cggccaggag cggctgacca
     6181 atccattcct caccggcgag atgtgagacc ggcgcgacgg cacgggcctc aacatatttg
     6241 tcgcgaggtt gttgatccgg tgatggcagc tattcggcgg cggccatttc aaccgtgttc
     6301 acctgtgcga ggtggcgcgt gccccagtcg cgaatgctgg cgatgacggg aacgaagctt
     6361 ttacccttgc gcgtcagccg atattccacc ttgggcggaa cgacgccgta atccctgcgg
     6421 tcgatcaggc cttgctcggt gagcgccttc aactcccggc tgagcacgcg gggcgcgatt
     6481 tccgaactgc catccgaacc gcgcagcaga ccggttctga tctcgccata gcgcttggga
     6541 ccatcctgca gatcccagac aatccgcagc ttgtatttgc cgctgatcat tttctggaac
     6601 gcggccaccg ggcacccgca cgtcagcgct gattttgtct tcgccatatc gtcgcctcca
     6661 accctgcctt tttcaggata ccggtaccgc aagcaaagtc catagtatca gttttgtcca
     6721 tacttgcgtt ttcgctccta ggagcgcaca gatgacgagc gtcaaccgga gatggaccat
     6781 gatcctcgtg acaggcgcca ccggactgaa cggaatggaa ttgctgcgcc ggctgtcggc
     6841 gcggggtgtt gccgtgcgtg cgctggtgcg cgatccggcc aaggccgagg cgatcgcagc
     6901 actgccgcat gtcgaaatcg cgcaaggcga catggcgcgc cccgaaacgc tcgcggcagc
     6961 cttgcatgag atcaatcgcg cgatgctgat ttcgtcctcg gcccctgaca tgttcgatgt
     7021 ccagtcgaac ttcatcgacg ccgcgcgcaa ggcgggcgtg cggcacgtcg tcaaactatc
     7081 cggcatcatg ccggggcttc actcggcgtt tcgcttcgcc cggatgcatg gcgagatcga
     7141 gaaccggctg gaagcctcgg gaatggccta cacccatctg cgggccggcg agttcatgcc
     7201 ggcctatttt cgccaggtcc cgccgatcgt cgccaagggc gcgatgttcc tcccgatgga
     7261 ggatgccagg atcgcgtcga tcgatgtcgg cgatatcgcc gaaatcgcgg cccgcgtgct
     7321 gaccggttcc ggacatgaag gcaaggccta tcccctcacc ggtccggagg cgctgaccat
     7381 gacggaggtc gccgcaaaac tctccaccgc caccggaaaa cccgttcgct atgtcaacgt
     7441 gccgccggag gatgcgaggc aagcccagct tgccgcgggc atgccgcctt acctcgccga
     7501 tgcattgttc gaattgtttg ccgagcggcg caacggcaag gagggcaagg tctggcccga
     7561 cgccgccacg ctgctcggac gtcctccgac atccttcgac gaattcgcca ggcgcaacgc
     7621 cgccttgttc cggggtgaag cgccgccgcc gaaagtttga cgtctgcgcc gatgaaggag
     7681 ccaccgatga agcacttcat gatcaaatat cagttcgcca acggcaccac ggaggcctgg
     7741 catcgggaaa tcgaaatatt catttccgcg ctcgaccacg atcccgacct gaagggaaag
     7801 atcagctacc gctgcatgaa gaaccgcgac gattccagct acatccatct cgccgcggcg
     7861 gtcgacgagc aggccatcaa gaccctgcag gcgcgggatt tcttcaagct ctataccgaa
     7921 aagacgaggc aagtcgccgg cggcgaggtc gtggtgacgc cgatcgaact gatcgcggag
     7981 acggcgtagc gaacagagag gcgctctttc ttccccctct ccccttgtgc cccttgtggg
     8041 agagggtggc gcgatacgaa cgcagtgagc atcgcgacgg gtgaggggtc acggtctatc
     8101 gatgagcgcc gcaacccctc atccggcacg gacttcgcat agccgatgca aagcatcggc
     8161 gtcctcttaa aggacggcgg ccgaaggccg cctatggcca ccttctccca caagggagag
     8221 gggacaagac ctaccagccc taacccgaaa tcatcgcgcg tatccgccgc gagagaatct
     8281 cgaggtcgaa cggcttggtg atcatctcca tgccgggctg cagaaaactg tccgacatcg
     8341 caacgctctc ggcgtagccg gtgataaaga gaatcttgag atcgggccgg gtttcgcgcg
     8401 cctgatcggc gagctggcgg ccgttcatgc cgggcaagcc gacgtcggtg accagaagat
     8461 cgacccgctc agggttgcgc aggatgcgca gccccgacgg tccatcgacc gcttccagcg
     8521 ttcggtagcc ctgatcagca agcatctcca gaatgacggc gcgcaccacc gcttcgtcct
     8581 cgaccaccag cacggtctcg ccggtgacgg cgtgttcggc tgccccgatc gccgatgcat
     8641 gctggcttgc gatatcgcca tgatgacgcg gcaaaaagag tttgaccgat gtcccctgcc
     8701 ccaatctgct gtcgatcgtc gcatgaccgt tcgactgccg tgcaaatcca tagatcatcg
     8761 aaagcccaag tccggtgcct tgcccgatcg gcttggtggt aaagaacgga tcgaaggcgc
     8821 gtgccgccac ctctgcgctc atgccggtcc cggtgtcggt gacgccgatg cagacataat
     8881 cgccgggcaa gagcgcgggg gagtccgcgg tcaccccatc gagccgcacg ttcgcggtcg
     8941 agatggtgag cttgccgccg ccgggcatgg cgtcgcgcgc gttgatggcg agattgagca
     9001 gcgcgctttc cagctgattg ggatcgcaca gcgtcaacca cagatcgttc gccgcgacga
     9061 tgtcaagatc gatggtctcg ccgatcgtcc ggcgcagcag atcctccagc gacaccacga
     9121 gctgattggc gtcgacactt ttcgggatca gcggctgtcg ccgcgcgaac gccagcaaac
     9181 gatgcgtcaa tgcggcggcg cggttcgccg atgtcatcgc cgcgttgatg tagcgcgtgg
     9241 cgttgtcggt ccgcccctga tcgaggcggg tctgcagcag atccagcgat ccgacgattc
     9301 cggtcaggag attgttgaag tcgtgggcga tgccgccggt caattgcccc accgcttcca
     9361 tcttctggga atgcagcaac gcctcctcgg cggccttgag ccgctccgcc gcggccttct
     9421 ccgcggtgac gtcgcgggcc accgcgtagg tggcgtcctc gtcggatacg ccggtccatg
     9481 acagccagcg gtacgacccg tccttgtgcc gaaagcggct ctcaaacctg acggtggttc
     9541 cgctcgtgcc aagctttttg aactgcgcgc gcgtcaggcc gccatcgtcg ggatgctcca
     9601 gccagtccga gctgcggtcc agcagttcgg cctcgctcca gccgagtgtt ctggtccagg
     9661 ccggattgac tgtgcgccag atgccgccct ggtccgcgac caccaacaga tcctgcgaca
     9721 cgttccagat ccggtcgcgc tcccgggtct tttcctcgac gcgctgcgcc agcgtcgcac
     9781 tcagcccggc gagttggtcg gccaacttgc ggtatcgctc ttcgctttgc cgcaacgcct
     9841 gctccgccat cacctgctcg gtgacatcgg tgtgggcgcc gaccaggcgg atggctcgcc
     9901 cctgcgcgtc gcgctcgatg gtggatctga ccgaaatcca gcgcgtctcg ccatcgctcg
     9961 gacgaatgat ccggtattgc gcattgtagt cgcgaacctt gcctgcgacg gcgtcgcgga
    10021 atttcttttc ggtggctgtt cgatcctcgg ggtgaatgcg gcggacccaa tcctcgtggg
    10081 attcgtcggc cgcttccggc ggcagcccgt gaatcagcaa gtactccggc gaccggcgat
    10141 tgcggaaacc ggttcggagg tcgacctcga ggccaccgat ccggccgatc tgttgcacgc
    10201 gcgccagctc ggcttcgcgt tcccgtaacg ccgccgcggc aagatattct ttggtcacgt
    10261 cacggcagac gaccagcaca ccgccaaccc gatcgtcttc gtcgatcggg ctgtagccgt
    10321 aggtccagta gacctgctcc agcctgccgt ggcgcgtcac cggaacgagt tggttctcat
    10381 gccacgtcgc gccgccgccg ctcatcacct gctcgatctg gggaccgata atgtcccaga
    10441 cttccgccca gcactcccgt ccgcgttgac cgagcgcgct cggatggcgc tcaggtccca
    10501 tggtctggcg ataggcgtca ttgtagaatt gaatgagctc ttcgccccac cagatgaaca
    10561 tcggatggtt ggtattcagc aggatgcgca acgccgtccg caaactttgc ggccaggcct
    10621 cgggagcgcc gagcgggctt gccgaccagt cgtaggcccg cgtcaaggcg cccatctccc
    10681 cgccgccggc gaggaaatcc gcatgagtcg gtccgttggt cttcaacatt caggacgatc
    10741 ggttggcgag ttcagcccaa attggcggcc ggaacttacc cccgcttcac aatagcggca
    10801 aactcacgcg acggcatctg gccccaaata tattttcaac ggatggaacc aaaccgggat
    10861 gccgtgcgcg cgcgccgcat taaccatcgc gatgaaacgc catggccatg cggcgccgat
    10921 cggcggcgca agccccgtta tttcatgagg cccgccgccg tgagcgcgcg ggtgatgacg
    10981 gcgccgacgt cgataccgcg acgccgcggc gcgcgcggtg gctcgggacg gcggctggac
    11041 tcgggacgac ggttgagcgt cgtgcgcggc cacagcaccg cggtgagatc cggcaccttg
    11101 acgggcgtcg gtgaaaccgc cggagttggc ttgacggctg gcttggcggt ttccttgacg
    11161 gtttccttga cggtttcctt ggtagtttct ttggcagctt ctgccggagc cacctcgggc
    11221 tggcgaatcc attcggtcag gccaaagaaa ttcgcaatgt gataggagga cgagattccc
    11281 gcctcgatca ggaacgcgcc ctgggcgccg tatcgctcgt cattgtcacc cacaccaagc
    11341 ggggtgccat gggccatatc ggtgatggtg taggattcga cgatggtttc gccgtcggcg
    11401 ttccaccaga cctggcgggg atagccgtcg acatcggcct ccgacatcgg cgccgcaggc
    11461 aattgatgca cgtcgagcca ctgcttgacg atctcattgg cgttggccgg gttgacggtg
    11521 cggtcggcgc tcccgtgcca caccgaaagc ttcggccacg gtcccctgtg attcgaagcg
    11581 ttgcgcacca gatcgcccaa ttcgcttgcg gggcgggatg gcgacacaaa catgccgctc
    11641 aaggcttccc gcacattggt cgccacgccg aacggcagcc ctgcgatgat ggcgccgccc
    11701 gcgaatattt cgggataggt cgcgagcatc accgatgtca tggcgccgcc ggcggagagc
    11761 ccggtcacga agatacgatg ctgatcgatt ttgtgatcgc caaccatccg cgcgatcatc
    11821 tgtcggatcg agcaggcttc cccgctgtcg cgcgcagtat cttccggatt gaaccagttg
    11881 aagcatccct gggtgttgtt cgatgatgcc tgctccggca tcagcaacgc gaaaccatag
    11941 tgtttcgcaa gcgtagacca gccggcaccg agatcatagc ccgcggcggt ctgaccgcat
    12001 ccgtgcagaa cgacaacaag cgcgggcgct ggctgcaggc tatcgggcac aaacgagaac
    12061 atcttgagag caccgggatt cgagccgaat ccggtgattt caaccagcgg gctgcggacg
    12121 cccggcctca ggtttcgcgc gaaatctcca aagccattga gaccttgcag ctttggcaga
    12181 cgtcggagga attcgacatt gttggcgagg gacaccggca gctccttgat agagttatct
    12241 taactccacc gaatacagat agttgctgca ctgcaaaatg aaaaggccgt gtgctgtcag
    12301 tccccagaat taaaaagacc gtgtgctgtc agtcttaagg attaaggcaa tcgatcttaa
    12361 tcggtgtcag gcggcagcgc gtgccgcatc catgacagaa acgcggcaca ggccacaatc
    12421 gacaccgcaa acagcgccga cgcagcgaac agcgccaatc gcgccgagcg agcggcttca
    12481 atcgcaaaaa aagctccgcc gatcgccgcg acgccggcag cgttggcgat ttgcgccgtc
    12541 gtgccataca ttcccgcgcc cgagccggcg ctcgccggct tcacggtgga tagcacggcg
    12601 ctcgacaacg gcgccatcac caacccctgg ccgtagccga agatcgccaa caccgacgcg
    12661 agcaggggcg cagcgggcgt cacgtcggtt gcaaccgtca gcaccagtac cgcgaggccg
    12721 acaatctgaa ccgcacagcc ctcgatcagc acgcgggtgc cgcgatgccg cgcccgcgca
    12781 ccgctgtgtc gcgaggcgat gacaaaggcg agcgccaaag ggaggaacac cgagccagcc
    12841 tgcagcggcg gaatccgcaa gccgcgctgc atgaacatcg tcatcaccag atagaacgac
    12901 aggttggcga agaagaaaaa gaaggcagca aacagaccgc gcatgaacgc ggcgtcggac
    12961 aacagcgcaa ggtcgatcag cggcatgcca tcgcggcttg cgacggcgcg ctcgagccgc
    13021 aggaacgcgg caacgatggc gacgcccgcc gccatgacca accagatccc tggcgcccaa
    13081 tgcacatcgt gaccgaacag caacgggccg atcaggcaca atagtcccgc gaacaacacg
    13141 aaagcgccgg gaacgtccag ccgcgtgccg gcgcggcgcg gcaccgacgg catgatccgc
    13201 agcgccgcca atatgatgat cagcccgaac ggcacgttga cgaaaaaaac cgcgcgccag
    13261 ccgaggccgg ccagatcaag cgtcaccagc acgccgccga gcaggaagcc ggccgcgccc
    13321 gcaaggccca gcacaatgcc gtagatgcca aaggcgcggc tgcgcgaggt gtccgaaaac
    13381 agcagatgaa gggtcgcgag cacctgcggc accatcagcg ccgcggtggc gccctgcgcc
    13441 agcctggcgg cgatcaattc ggggccggat tgcgcgagcc cgcaccacag cgaggtgacg
    13501 ctgaagccga acacgccggc caaaaataca tttttggtgc cgtggatgtc gccgagccgg
    13561 ccgccggtga ctaccagcgt tgcataggcg atgaggtaga tcgcgatcac cgcctcgatc
    13621 tgcgccgggc ttgcatgcaa ttcgactgca atggtcggga tcgcgacatt gacgatgaag
    13681 gcatcgaccc cgaacatgaa ttgcgcggcg acgacgatcg ccagcaccca ccagcggcgc
    13741 gatgaatcga cgggggcgga aacaatctgg tgcatgggaa gcggcctcgg ttcgaaatct
    13801 gtcgcttcga ttgatctcag gttgcagtcg tcgcagcgat tacctccgag gtaaggaatc
    13861 gcggcagctt ggcccgattg ctgcatggaa actgtcatgg acagctctcc agcctgtcgc
    13921 gcgaggtcgg gaccgcctgc gatgtatgat tatgacatgg tggtgatcgg cttcggccca
    13981 tccggccgcc gcgccgccgt gcaatttgcc aagctcggca agtcagttct ggtggtggaa
    14041 aagggccggc gggtcggcgg cgtttcggtc cataccggca ccatcccgtc caaaaccctg
    14101 cgcgagaccg tgctcaatct ttcgggctgg cgcgagcgcg gcttttacgg ccgctcctac
    14161 cgggtgaaac aggatatcgg cgccaccgac ctgatcgcgc ggctgcaaaa gaccctcgat
    14221 catgaagtcg aagtgctcga acatcagttc agccgcaaca tggtcaagac cgcctcgggc
    14281 gagggccgtt ttctcgggcc gaacaagatc gagatcaatg cgggcggcg
//
LOCUS       DATNOI010000058        10442 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_163114,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000058 DATNOI010000000
VERSION     DATNOI010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10442
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163114"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2079)
                     /locus_tag="VN968_01975"
     CDS             complement(<1..2079)
                     /locus_tag="VN968_01975"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HXL28049.1"
                     /translation="MNDMTPTREQREAKLEGMGCKRKRVEDARFTQGKGNYVDDLKLP
                     GMLHGDFVRSPHAHARVKSIHSEEALKVPGVLAVITAETLKTVNLAWMPTLAGDVQMV
                     LADGKVLFQNQEVAFVVATDRYAADDGINKVVVEYEPLPVVIDPFKAMDADAPVLRED
                     LAGKTTGAHGPRKHHNHIFEWTVGDKDLTDAAFRKADVTIKEMISYHRTHPSPLETCQ
                     CVCSFDKIKGELSIWGTFQAPHVIRTVVALIAKIPEHKIHVISPDVGGGFGNKVGAYP
                     GYICAAVASIVTGKPVKWVEDRIENLTATSFARDYHMTTEIASTKDGKVTGLRVHVLA
                     DHGAFDACADPSKWPAGFFNIVTGSYDFPVAHLSVDGIYTNKAPGGVAYRCSFRVTEA
                     AYCIERGMDILAQKLGMDPAELRLKNFIKPEQFPYHSALGWEYDSGDYHTAMKKMMET
                     VDYAGLRKEQAEQRAAFKRGETREIMGLGVSFFTEIVGAGPSKNCDILGIAMFDSCEI
                     RLHPTGAGIARVGTKSTGQGHETTWAQIIATEIGIPADDIMVEEGNTDTAPYGLGTYG
                     SRSTPVAGAAIAMAARKIKAKAQMIAAYKLEVHEGDLEWDIDSFRVKGLPEKSMSMKD
                     ICWAAYNSVPPGMEPGLEAVSYYDPPNMTYPFGAYICVMNIDVDTGVYKIRRFYALDD
                     CGTRINPMIIE"
     gene            complement(2076..2576)
                     /locus_tag="VN968_01980"
     CDS             complement(2076..2576)
                     /locus_tag="VN968_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL28050.1"
                     /translation="MAKTHVTMKVNGAEVEGLVEPRTLLVHFIRENLLLTGTHIGCET
                     THCGACTVDLDGMSVKSCTMFAVQAQGAEITTIEGVANADGTLSALQEGFRTMHGLQC
                     GFCTSGMILRAQRLLRENPSPTEAEIRMGISGNICRCTGYQNIVKAIQYAADKINGVE
                     FKEAAE"
     gene            complement(2588..3451)
                     /locus_tag="VN968_01985"
     CDS             complement(2588..3451)
                     /locus_tag="VN968_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXL28051.1"
                     /translation="MIPGPFSYHRPATVADAVKLLSTLGDEARPLAGGHSLVPMMKLR
                     LATPEHLVDLHGIDGLKGIRREAGRIVIGAMTTQHELLASDDIAKSLPILHETAMLIA
                     DPQVRYMGTIGGNVANGDPGNDMPALMLTLGASYRLEGPSGGRAIAASEFYQGAYFTS
                     LEPGEILTAISIPTPAAGHGYAYEKLKRKVGDYATAAAAVVLTMASGKVASCAIGLTN
                     LHETPLLAADAAKLVIGTSLDAATLKKAAAAAEAIMSPAADARGPVEYRKHVGGIMVT
                     RALQRAAARAS"
     gene            complement(3593..4855)
                     /locus_tag="VN968_01990"
     CDS             complement(3593..4855)
                     /locus_tag="VN968_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HXL28052.1"
                     /translation="MFEGHDPYLVALSVVIAILGGYTGFGLAARIRGTPGVSRRVLLA
                     GAAAFLAVGIWTMHFVGMLAAPIPADTVYLVLPTIVSFLICALVVGISLFFVSIGEPS
                     LRRVISSAVLLGAGIASMHYVGIHGLAGDFAIAHDNSMIFLSVLVAIVAAYGGLRAFL
                     ARQDGVRLIVSSIAFGVAVSGMHYTAMAGMHFAALSGEAHHHGGGLAASQQILSVVVA
                     LLCFVIAAGFLLSLVPDPRRQTMAIAAVVTSPLGEAGVAAAPPSSPDPVPVAAPVARP
                     MSAPLGGLGQPPRPAPAPRLPVEGANGTHFIDAAEIRSVRADAHYTRIHDGTRERMCP
                     WSISEAEAQLDPGLFVRVHRSHIVAIPHVSFVRKEGDGAIIELDGPSPHRVPVSRAKI
                     AEVKARLGLARRNAQPYQTASGRQGRED"
     gene            complement(5169..5771)
                     /locus_tag="VN968_01995"
     CDS             complement(5169..5771)
                     /locus_tag="VN968_01995"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HXL28053.1"
                     /translation="MNQMLQDAPQVQEVPELEAPEVNDSPKAGAETIDRPPVPAHPRF
                     AKPQRVAFVQSAWHREVVEECRLAFLEEIEARHIARAQIDLFEVPGSFEIPLHAQLLA
                     KTRRYTAIVAAGLVVDGGIYRHEFVADTVIKALMDVQLRTEVPVFSAVLTPQQFHETA
                     AHFEFFRKHFATKGVEVAEACANTLLSLERLRGQVAAGIV"
     regulatory      complement(5840..5992)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6477..6977
                     /locus_tag="VN968_02000"
     CDS             6477..6977
                     /locus_tag="VN968_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HXL28054.1"
                     /translation="MSLLEGIIDSPTNPLAVVEDIAADNNWSFERSGEDEVTIVSKGE
                     WTDYQLSFTWMTEIEALHLACAFDMKVPTARRAEVQRLIAAINEQLWIGHFDIWTHTG
                     MIMYRQALVLPGGVTASSAQCEAMLVGAIHACERYYPAFQFVVWAGKSAAEAMSAAMF
                     DTEGEA"
     gene            7292..8125
                     /gene="proC"
                     /locus_tag="VN968_02005"
     CDS             7292..8125
                     /gene="proC"
                     /locus_tag="VN968_02005"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HXL28055.1"
                     /translation="MASPTTSALQNIKTTIVLAGAGKMGGAMLTGWLTQGLDARRVVV
                     IEPYPSDEITALVTKGIGLNPSPKTIGAVATLVIALKPQSFREAGAVLKSFVGSSTLA
                     VSIMAGTTIASISEVCGGSVVRAMPNTPAAIGRGITVAVAASTVSAAQRAVADALLRA
                     TGSVEWIDDENLMDAVTAVSGSGPAYIFLLAEELARAGVEAGLPPELATRLARETVAG
                     SGELLHRSKTSSATLRQNVTSPGGTTAAALEVLMGPDGMQSLLTRAVAAATRRSKELA
                     K"
     gene            complement(8126..8521)
                     /locus_tag="VN968_02010"
     CDS             complement(8126..8521)
                     /locus_tag="VN968_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HXL28056.1"
                     /translation="MDARDIIKIGMSAERMLVVPPERTVGHFVPDMPMVYATPMMILE
                     MELASGNAIDACLEPGWVTLGTEVDIRHLAASPVGALVRTTARVIAVQRRVIRFEVEA
                     FDGERRIGEGRHARGLVNLAKFIQRLGAR"
     gene            complement(8714..8917)
                     /locus_tag="VN968_02015"
     CDS             complement(8714..8917)
                     /locus_tag="VN968_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HXL28057.1"
                     /translation="MPEITISMAAGRTDEQKAGMMRDITQALVTNLGVDADAVVIQIN
                     EAPLAHKMKGGKTFVERKAMQSK"
     gene            complement(9032..10442)
                     /gene="hisS"
                     /locus_tag="VN968_02020"
                     /pseudo
     CDS             complement(9032..10442)
                     /gene="hisS"
                     /locus_tag="VN968_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090191.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="histidine--tRNA ligase"
BASE COUNT         1910 a         3333 c         3213 g         1986 t
ORIGIN      
        1 ctcgatgatc atcgggttga tgcgggtgcc gcaatcgtcg agcgcgtaga agcgcctgat
       61 cttgtagacc ccggtatcga cgtcgatgtt catcacgcag atataggcgc cgaacggata
      121 ggtcatgttg ggcggatcgt agtagctcac cgcctccagc cccggctcca tgcccggcgg
      181 caccgaattg taggccgccc agcagatgtc cttcatcgac atcgatttct ccggcagacc
      241 tttcacccga aaactgtcga tatcccattc gagatcgccc tcgtgcacct cgagcttgta
      301 ggcggcgatc atctgcgcct tggccttgat tttccgcgcc gccatcgcga tcgcagcgcc
      361 cgccaccggc gtggagcgcg aaccataggt gcccagcccg tagggcgcgg tatcggtgtt
      421 gccctcctcc accatgatgt cgtcggcggg aatgccgatc tcggtggcga tgatttgcgc
      481 ccaggtggtc tcatggccct ggccggtgct tttggtgccg acccgggcaa tgccggcgcc
      541 ggtgggatgc aacctgatct cgcaggaatc gaacatcgcg atgcccagga tgtcgcaatt
      601 cttcgacggg ccggcgccga cgatctcggt gaagaacgac acgccgaggc ccatgatctc
      661 gcgggtctcg ccgcgcttga acgcggcgcg ctgttccgcc tgctccttgc gcaggccggc
      721 atagtcgacc gtctccatca tcttcttcat ggcggtgtga taatcgccgg aatcatattc
      781 ccaccccagc gccgagtgat acgggaattg ttcgggcttg atgaaattct tcagccgcaa
      841 ttccgccgga tccatcccga gcttctgggc caggatgtcc atcccgcgct cgatgcagta
      901 ggccgcctcc gtcacccgga acgagcagcg ataggcgacg ccgcccggcg ccttgttggt
      961 gtagatcccg tccaccgaca gatgcgcgac cgggaaatca taggaccccg tgacgatgtt
     1021 gaagaaaccg gccggccatt ttgacggatc ggcgcaggcg tcgaacgcgc cgtgatcggc
     1081 cagcacgtgg acgcgcagac cggtgacctt gccgtccttg gtcgacgcga tctcggtggt
     1141 catgtggtag tcgcgcgcaa acgacgtggc ggtgaggttc tcgatacggt cctcgaccca
     1201 cttgacgggt ttgccggtga cgatcgaagc cacggcggcg cagatgtagc cgggataggc
     1261 gccgaccttg ttgccaaaac cgccaccgac gtcgggcgag atcacatgga tcttgtgctc
     1321 cgggattttc gcgatcagcg ccaccacggt gcggatcaca tgcggcgcct gaaacgtgcc
     1381 ccagatcgac agctcgccct tgatcttgtc gaacgagcag acgcactggc aggtctccag
     1441 cggcgacgga tgggtgcgat gataggagat catctccttg attgtcacat ccgccttgcg
     1501 gaaggcggcg tcggtgagat ccttgtcgcc caccgtccac tcgaagatgt ggttgtggtg
     1561 cttgcgcgga ccgtgcgcgc ctgttgtctt gcccgcgaga tcctcgcgca gcaccggcgc
     1621 gtccgcgtcc atcgccttga acggatcgat cacaactgga agcggctcgt attcgaccac
     1681 gaccttgttg atgccgtcat cggcggcata acggtcggtg gcgacgacga aggcgacttc
     1741 ctggttctgg aacagcacct tgccgtcggc cagcaccatc tgcacgtcgc cagcgagcgt
     1801 cggcatccag gcgaggttga cggtttttag cgtctcggcg gtgataacag ccaatacgcc
     1861 cggcaccttc agcgcctctt cgctatggat cgacttgacg cgcgcatggg catgcggcga
     1921 tctaacaaaa tcgccatgca gcatgcccgg cagcttgagg tcgtcgacat aatttccctt
     1981 gccttgggtg aagcgggcgt cttcgacccg cttgcgcttg cagcccatgc cttcgagttt
     2041 ggcttcgcgt tgttcccgcg tgggagtcat gtcgttcatt ccgcagcctc cttgaattcg
     2101 acgccattga tcttgtcggc agcgtattgg atcgccttga cgatgttctg atagccggtg
     2161 cagcggcaga tattgccgga gatgcccatc cggatttccg cttcggtcgg cgatggattt
     2221 tcccgcagca gccgctgcgc gcgcaaaatc atgccggacg tgcagaagcc gcattgcagc
     2281 ccgtgcatcg tgcgaaagcc ttcctgcaac gccgacagcg tgccatcggc attggcgacg
     2341 ccctcgatcg tggtgatctc ggcgccctgg gcctgcaccg cgaacatggt gcagcttttc
     2401 accgacatgc cgtcgagatc gacggtgcag gcgccgcaat gggtggtctc gcagccgata
     2461 tgggtgccgg tgagcagcag gttttcgcgg atgaaatgca ccagcagcgt gcgcggttcg
     2521 acgaggcctt cgacctcggc gccgttcacc ttcatggtca catgggtttt tgccatttgt
     2581 ttcctcctca gcttgcgcgc gccgcggcgc gttgcagcgc ccgcgtcacc atgatgccgc
     2641 cgacatgctt gcggtattcg accgggccgc gagcgtccgc cgccggcgac atgatggctt
     2701 cggcggcagc cgcggccttt ttcagcgtcg ccgcatcgag gctggtgccg atcacgagct
     2761 tcgcggcgtc ggccgcgagc agcggcgtct cgtgcaggtt ggtaagaccg atcgcgcagc
     2821 tcgcgacctt gccgctcgcc atcgtcagca cgaccgccgc cgccgcggtg gcgtagtcgc
     2881 cgaccttgcg cttgagtttt tcgtaggcat agccatgacc ggccgcaggc gtcggaattg
     2941 agatcgcggt caggatttcg ccgggctcga gcgaggtgaa ataggcgccc tggtagaact
     3001 cggacgccgc gatggcgcgc ccgccggaag ggccttcgag ccggtagctg gcgccaagcg
     3061 tcagcatcag cgccggcatg tcgttgccgg gatcgccatt ggcaacgttg ccgccgatcg
     3121 tgcccatgta acgcacctgc ggatcggcga tcaacatcgc ggtttcgtgc aggatcggca
     3181 gcgatttggc gatgtcgtcg gacgccagca gctcgtgctg ggtggtcatg gcgccgatca
     3241 cgatgcgacc cgcctcgcgg cggatgcctt tcagcccatc gatgccatgc agatcgacca
     3301 gatgttccgg cgtcgcgagc cgaagcttca tcatcgggac cagactatgg ccgccggcaa
     3361 ggggacgggc ctcgtcgccc agggttgaca gtaatttgac cgcatcggcc acggtcgcgg
     3421 gccggtgata gctgaatggt ccaggaatca tcgtctcctc catgcgtctt cttgagtgca
     3481 gacgttgggc ggaggctgtc cccggatttt ttcgcaggca aggcgaagcc ggtcgtccgg
     3541 tcacaaggtg agagaattcg cacgaaacgc cgtttcgctc gcacgaaatg cgtcagtctt
     3601 cgcgaccttg gcgacccgat gcggtttgat acggctgcgc gttgcgtctg gcgagaccaa
     3661 gccgggcctt gacctcggcg atcttggcgc ggctgacggg cacgcgatgc ggcgatgggc
     3721 catcgagctc gatgatcgcg ccatctccct ccttgcggac gaagctgacg tgggggatcg
     3781 ccacaatgtg gctgcgatgc acccggacga acaggccggg atcgagctgg gcttcggcct
     3841 ccgaaatcga ccacgggcac atccgctccc gggtgccgtc atggatccgg gtataatgag
     3901 cgtcggcccg gacgctcctg atctcggcgg catcgatgaa gtgcgtgccg ttggcgccct
     3961 cgaccggaag ccggggcgcg ggcgcggggc ggggcggctg gccgaggccg ccgagcggcg
     4021 ccgacatcgg ccgcgccacg ggtgcggcaa ccggaaccgg atcgggcgat gacggcggcg
     4081 cggcggcgac gcccgcctcg cccaacggac tggtcacgac ggccgcgatc gccatcgtct
     4141 gccgccgcgg atccggcacc agcgacagca ggaagcctgc ggcaatgaca aaacacagca
     4201 gcgccaccac caccgagagg atttgctgcg atgcggccag tccgccgcca tggtgatgcg
     4261 cctcccccga cagcgcggcg aaatgcatgc cggccatcgc ggtgtagtgc atgcccgata
     4321 ccgcgacgcc gaacgcgatc gagctcacga tcagccggac gccgtcctgc cgggccagga
     4381 acgcgcgcaa gccgccatag gcggcgacga tcgcgaccag caccgagagg aaaatcatcg
     4441 aattatcatg cgcgatggcg aaatcgccgg cgagaccgtg aatgccgaca taatgcatgc
     4501 tggcgatgcc cgccccgagc agcaccgccg acgagatcac gcggcgcagc gagggttcgc
     4561 caatgctgac gaaaaacagg gatatgccga ccaccagcgc gcagatcagg aacgatacga
     4621 tggtgggaag aacgaggtag acggtatccg ccggtatcgg cgccgccagc atgccgacaa
     4681 aatgcatggt ccagatgccg accgcgagga acgccgccgc acccgcgagc agcacgcgac
     4741 ggctcacgcc cggcgtcccg cggatacgcg ccgcgaggcc gaaaccggtg tatccaccca
     4801 ggatcgcgat caccaccgag agcgcgacga gatagggatc gtgtccttcg aacatgatcg
     4861 tctcggccgc ccgtctccac gggcgtgcct cctcccgccg ccgagtttat aggcggctgt
     4921 gaaaatgaca atcaatccgt cttagtgcgt gacaaagcga ggttgaacgg cagagcgaca
     4981 atgaatccct tctccctcgc cccgcccttg cggggagagg ggcttttcta tctgcgagtc
     5041 ctgaaaaggt ggagaggggg gcgccccctc acccggatca catctgggat gtgatccgac
     5101 ctctccccgc aggcggccgc aggcggggag aggtgacaca gttcacgcgc cgccgctgga
     5161 gcctccgcct acacaatccc cgccgcgacc tgaccgcgca gccgttccag gctgagcagc
     5221 gtgttggcgc aggcttccgc gacctcgacc cccttggtcg caaaatgctt gcggaagaat
     5281 tcgaaatgcg cggccgtctc atggaactgc tgcggtgtca gcaccgccga gaacaccggc
     5341 acctcggtgc gcaactgcac atccatcagc gccttgatca cggtgtcggc gacaaattcg
     5401 tgccggtaga tgccgccatc gaccacgagc ccggcggcga cgatcgcggt gtagcgccgg
     5461 gtcttggcca gcagttgggc gtgcagcgga atctcgaacg agccgggcac ctcgaacaga
     5521 tcgatctgcg cgcgcgcgat gtggcgcgcc tcgatctcct cgaggaaggc gagccggcat
     5581 tcctcgacca cctcgcgatg ccaggcggac tgcacgaagg cgacccgctg cggctttgcg
     5641 aagcgcgggt gtgctggaac cggcggacga tcgatcgttt cggcaccagc tttgggagag
     5701 tcgtttactt cgggagcttc tagttcggga acttcttgta cttggggagc gtcttgcaac
     5761 atctgattca tggctttcct ctttcaggac cagaatcagg gcacacggaa cgacgcgccg
     5821 cgcggaatgc ggctgtcgcg accgttctct ttcatccgga ctttaaccgt cggcttcgga
     5881 atcacaccga atctgctgac ccttcttccc acaagagaag aaggcgctcg cgggcttggg
     5941 cttctcgacc cttaccgccg gtggggactt tcaccccgcc ctgagaacat cggccgcccg
     6001 ggttgaacgg cctaaaggga actatgacga aaggccggga cgtcagcaag caccttcacc
     6061 atggggaatc tgcatgtcgc catgacgcca aggctaggct tactaaatct agtctccagg
     6121 tcgggccgga cccaaaaaaa tcaacgaaaa attaacgatt aaggcgggcc aacccatccg
     6181 cggacacgaa ttcgccgcac ctgactcggt ctgtgcgaat ccgattccgg tttgttctca
     6241 aattaataat caggtcgcgc gatcagctgt ggacagtgca ccgctttggc tgtggacgga
     6301 ctccccgtgc ggttgcgccg attccgcaaa tcacatcact tgatcccggc agaccccggg
     6361 gacgatcgat caggggtttg cagtcggcga cgatgcgtca ggtcagcacg tgtcggcctg
     6421 ctgcgtgcgt gatcaaccat cgtatgttct ttcaaaaaag ataaggccga gacggcatgt
     6481 ccctcctcga aggcatcatc gactccccaa ccaatccact cgcggtggtc gaggatatcg
     6541 ccgctgataa caactggtcg ttcgaacgct ccggcgagga cgaggtgacc atcgtctcca
     6601 agggcgagtg gaccgactac caattgtcct ttacctggat gaccgagatc gaggcgctgc
     6661 atctggcctg cgccttcgac atgaaagttc cgaccgcgcg ccgcgccgag gtgcagcggc
     6721 tgattgccgc gatcaacgag caattgtgga tcggacattt cgacatctgg acccacaccg
     6781 gcatgatcat gtaccggcag gcgctggtgc tgccgggcgg cgtcaccgcc tcgagcgcgc
     6841 aatgcgaggc catgctggtc ggcgccatcc acgcctgcga acgctattat ccggcgttcc
     6901 agttcgtggt ctgggccggc aaatcggcgg ccgaagccat gagtgcggcg atgttcgaca
     6961 ccgagggcga ggcgtaacgc gcgcgctcta ttcctcagcc gtcatgcccg ggcatggcag
     7021 tctgccttgc gcagactgcg taaacttgtc tgcgatcccg cgcatccatc caacaagagt
     7081 cattccaagc gatggattgc cgggtcaagc ccggcaatga cggtaatagt cgtcgtcccg
     7141 gccaagcgag catcgcgagc gcgagccggg atccatacgc cgtgtcgcca acgttcaagc
     7201 aaagtgcttg tggcctatcc tttgcttcac tagaaacgcc aggggttatg ggtcccggcg
     7261 ttcgccggga cgacgcaggt agttgtttct gatggcttcg cctaccacaa gcgctttgca
     7321 aaatatcaaa accaccatcg tcctcgccgg cgccgggaag atgggcggcg cgatgctgac
     7381 gggctggctg acgcaaggcc tcgatgcccg gcgtgtcgtg gtgatcgagc cctatccctc
     7441 cgacgaaatc accgcgctgg tgaccaaagg cattggtctc aatccatcgc caaaaacaat
     7501 cggcgccgtc gcgacgctgg tgatcgcgct gaagccgcag agctttcgcg aggccggcgc
     7561 ggtgctgaaa tcttttgtcg gttcgtccac gctcgcggtc tcgatcatgg ccggcaccac
     7621 catcgcctcg atctccgagg tgtgtggcgg cagcgtggtg cgcgccatgc ccaacacgcc
     7681 ggcggcgatc ggccgcggca ttaccgttgc ggtggcggca agcaccgtca gcgcggccca
     7741 gcgcgcggtc gccgacgcgc tgctgcgcgc caccgggtcg gtggaatgga tcgatgacga
     7801 gaatttgatg gacgcggtga cggcggtctc cggctcgggt ccggcctata tttttctgct
     7861 ggccgaagaa ctggcgcgcg ccggggtcga agctggtctg ccgccggaac tcgcaaccag
     7921 gctggcgcgc gaaaccgtcg ccggctccgg ggaattgctg catcgctcga aaacctcctc
     7981 cgctacgctg cgccagaacg tcacctcgcc cggcggcacc accgccgcgg cgctggaagt
     8041 gctgatgggg ccggacggca tgcaatcgct gctgacgcgt gccgtcgcag cagcgacccg
     8101 gcgatctaaa gagctggcga agtagctaac gcgcgccgag gcgctggatg aatttcgcga
     8161 ggttgaccag accgcgggcg tgacggccct cgccgatccg ccgctcgcca tcaaaagctt
     8221 cgacctcgaa gcggatcacc cggcgctgca ccgcgatcac ccgcgccgtg gtccgcacca
     8281 atgcgccgac cggcgacgcc gcgagatggc ggatatcgac ctcggtgccg agcgtgaccc
     8341 agcccggctc cagacaggcg tcgatggcgt tgccggacgc cagctccatt tccaagatca
     8401 tcatcggggt cgcatagacc atcggcatat caggaacaaa atgcccgacg gtgcgctccg
     8461 gcggcaccac cagcatgcgc tcggcgctca tgccgatttt gatgatgtcg cgtgcatcca
     8521 tgggatgatc cgaaattgat cacggcgtcg tcccggcgaa ccaacgggtc cggctattgc
     8581 cggcccgatg gcaggctctg ggacccatac gccgtgccgt cagcgttcaa gtagagcgta
     8641 cgtggcctgc cccgcgcttc accgaaacgc caggggttac gggtcccggc attcgccggg
     8701 acgaccagca gcgctacttg ctctgcatcg ccttgcgctc cacaaacgtc ttgccgccct
     8761 tcatcttgtg ggccaatggc gcctcgttga tctggatcac gacggcatct gcgtcgacgc
     8821 cgaggtttgt caccagcgcc tgggtgatgt cgcgcatcat gccggccttc tgttcatcgg
     8881 tgcggccggc ggccatgctg atggtaattt caggcatttt tggtcactcc ctttgctctt
     8941 gctacaaaaa ctctaacctg accccgaaga ggcgcaaggc gcaattcatt acgctcatta
     9001 ttgcggacgt gcggcgcttc tcgcctatga cgtggcgtgc aagaacttcg cgcaccttga
     9061 cgacgagttc ctcttcctta cacgaaaact gtgcgggacg cgtttcgcgc cattctgcat
     9121 tggacgcaat cgctgccgcg gccttggcgc catcgatcag atccttgatt tgcacctcgc
     9181 cccgcgcctt ctcgtccgaa ccttgaatga tgacgcaagg cgaattgcgt ttgtcggcat
     9241 atttgagctg gttgcccatg ttcttgggag tcccgagata aagttcggca cggatgttgg
     9301 cgctgcgcaa ggccgcaacc attttctgat aatcggcaat ccgaccctta tcgaacacag
     9361 ttacgacgac aggaccgaat tccggcgcgg tatcgagctt gccgagcatc gtgagcgccg
     9421 cctgcagccg cgacacgcca atggagaagc ccgtcgcggg caccgcctca ccacgaaacc
     9481 gcgacaccaa accgtcatag cgcccgccgc cgccgaccga accaaaacgc accgggcggc
     9541 ctttttcgtc tttggtgtcg agaagcagtt caacctcgta gacggggcca gtgtaatatt
     9601 cgaggccgcg cacaacggat gagtcaattt taattcgatc ctcgtcgtat cctgccgccc
     9661 gaacaagttt cgcaatctct gtaagctcag ttctccctgc ttcaagaagg ctctgacctt
     9721 ccattgcaat aaacggcttt ggcaattcgc cttgaatgcg ctcaaatgaa attccctcgg
     9781 caatttcttt gaactcgatc ttgaaattga aggtctggaa gaccgcctcg atttgaagag
     9841 ggtttagctc cgcacccctt gtaaagtcgc cttttccttc ttctccgcca tcccatcttc
     9901 caggaccgag caacttgcga acttcgtcag cggaaaattt atctagcttg tcgatcgccc
     9961 ttagtaccgt aagccgccgt cccgcgttct catcgccgcc aaggccgatg ctttccatta
    10021 ccccatcgag caccttgcga ttattcactt tgacgacgta atgccctttc aagcccagtg
    10081 cttccatggt gtccgctgcc atcatgcaca tctcggcgtc ggcagccgcg gcagccggac
    10141 cggatagggc ggtgcctatc gtatcggcgt cgaactgcat gaactggcgg aagcggccgg
    10201 ggcccggctt ctcgttgcga tagacgtaac ccgcgcggta actgcgatag ggaagaacta
    10261 gattttccgt tccgtaattt tccgcgacat agcgcgccaa gggcgcggtc aggtcgtagc
    10321 gcaggctgat ccactgttcg tcgtcgtcct ggaacgagaa cacgccctcg ttgggacggt
    10381 cctgatccgg caaaaatttg ccgagcgcgt cggtgaattc catcgctggg gtttccaccg
    10441 gc
//
LOCUS       DATNOI010000059         4051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_688439,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000059 DATNOI010000000
VERSION     DATNOI010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688439"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VN968_02025"
     CDS             complement(<1..456)
                     /locus_tag="VN968_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HXL28058.1"
                     /translation="MPHLTSLLGFALVSLGIGLTPGPNMIYLISRSITQGRGAGLISL
                     GGVALGFVFYMLCAAFGITALLFAVPYAYEALRLSGAAYLLWLAWQALKPNGRSPFQV
                     KEFAVDGPRKLFAMGFVTNLLNPKIAMLYLALLPQFIDPAQASVLTQALA"
     gene            complement(762..3839)
                     /gene="polA"
                     /locus_tag="VN968_02030"
     CDS             complement(762..3839)
                     /gene="polA"
                     /locus_tag="VN968_02030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HXL28059.1"
                     /translation="MPKTSEQTLPKAVAVKALAKGDHVFLVDGSSYIFRAYHALPPLN
                     RKSDGLQVNAVLGFCNMLWKLLRDMPPDNRPTHLAIIFDKSEITFRNKLYPDYKAHRP
                     PAPDDLIPQFPLIREAVRAFDLPCLEQGGFEADDLIATYAREAGERGATATIVSSDKD
                     LMQLVTDKVTMFDTMKDRRIGIAEVIEKFGVPPEKVVEVQALAGDSTDNVPGVPGIGI
                     KTAAQLIVEYGDLETLLLRAGEIKQPKRREALIENAEKARISRQLVLLDDKVKLDVPL
                     DELAVHEPDARKLIAFLKAMEFSTLTRRVAEYSQVDPADVEADAGSKSGASVFSSLPL
                     AEKVDELGNALDGDLFAGQSAPPRDQVEGKPSPANARGSAPGKTDGGKEDKAGSNKGT
                     PISLATARAEAARKTKIDRSKYQTIRSLEELRWWIARVHDVGHFAIEAKANSVDPMQA
                     EICGIALALGASDAAYVPLGHKQSGDGSGLFAAGLAPEQIKADDALEALRPLLESAGI
                     LKIGFNIKFNAVMFAQAGITIRNHDDAQLMSYALDAGRNAHGLDALAQTWLGHAVLTD
                     SDVIGSGKNKLSIDQVAIDKFTAHAAEDADVILRLWRVLKPRLVAERMTAVYETLERP
                     LISVLARMERRGISIDRQVLSRLSGEFAQTAARVEAELQEIAGEPINVGSPKQIGDIL
                     FGKMGIPGGSKTKTGAWSTSAQILDELAEQGHEFPKKILEWRQVSKLKSTYTDALPTY
                     VHPQTHRVHTTYALAATTTGRLSSNEPNLQNIPVRTEDGRKIRRAFIASPGHKLVSAD
                     YSQIELRLLAEIADIPVLKQAFRDGLDIHAMTASEMFGVPIKDMPGEVRRRAKAINFG
                     IIYGISAFGLANQLGIAREEASAYIKKYFERFPGIRAYMDQTRDFCRAHGYVETLFGR
                     KCHYPDIKASNASIRSFNERAAINARLQGTAADIIRRAMIRMEGALAEKKLSAQMLLQ
                     VHDELIFEVPDDEVAATLPVVQHTMQDAPFPAVLLSLPLQVDARAANNWDEAH"
BASE COUNT          712 a         1213 c         1306 g          820 t
ORIGIN      
        1 cgccagcgcc tgcgtcagca cgctggcttg cgccggatcg atgaactgcg gcaacagcgc
       61 cagatacagc atcgcgatct tcgggttgag cagattggtg acaaatccca tcgcgaacaa
      121 tttgcgcggc ccgtcgacgg cgaactcctt cacctgaaac ggcgagcggc cgttgggttt
      181 gagcgcctgc cacgccagcc acagcagata tgcagcaccc gagagccgca gcgcctcata
      241 ggcatagggc accgcgaaca acagcgccgt gatgccgaac gccgcgcaca gcatgtagaa
      301 cacaaaaccg agcgcgacgc cgccgagcga gatcagcccg gctccgcggc cttgcgtgat
      361 cgagcgcgag atcaaataga tcatgttcgg tcccggcgtc agcccgatgc cgagcgagac
      421 aagggcaaag ccgagcaacg aggtgagatg gggcatcttt ggaattctcg caggcaggag
      481 atgcaggttg tttaacgtgc atcaggccgc ggcgccattt cgtaatcggc gttgttgttg
      541 gtcgattatt tcagcgcaac gcctcggcta tcgctctgct ccctcgcccc gctcttgcgg
      601 ggagagggtt ggggtagggt tggggtgagg ggctctccga tccgcaaatg ttgaagaagt
      661 attgagcgcg gtaccccctc acccggatcg caagggcgat ccgacctctc cccgcaggcg
      721 gccgcaggcg gggagaggtg aagaaatagc ggcttaatca ctcaatgcgc ctcgtcccaa
      781 ttgttcgccg ctcgcgcgtc gacctgcagc ggcagcgaca gcagcaccgc cgggaatggc
      841 gcgtcctgca tggtgtgctg caccaccggc agggtcgcag cgacctcgtc gtccggcacc
      901 tcgaaaatca gttcgtcgtg gacctgcaac agcatctgcg ccgagagttt tttctcggcc
      961 agtgctcctt ccatccggat catggcgcgg cggatgatgt cggcggcggt gccttgaagg
     1021 cgtgcgttga tcgcggcgcg ttcgttgaag gagcggatcg aagcgttgga ggccttgata
     1081 tcgggataat ggcactttct gccgaacaag gtctcgacat agccatgcgc gcggcagaaa
     1141 tcccgcgtct ggtccatgta ggcgcggatg ccgggaaaac gttcaaagta tttcttgatg
     1201 taggcggagg cttcctctcg ggcgatgccg agctggttgg ccagcccaaa cgccgaaatg
     1261 ccgtagatga tgccgaaatt gatcgccttg gcgcggcggc gcacttcgcc cggcatgtcc
     1321 ttgatcggca cgccgaacat ttccgacgcg gtcatggcgt gaatgtcgag cccgtcgcga
     1381 aacgcctgct tcagcacggg aatgtcggcg atctccgcca ggaggcgcag ttcgatctgc
     1441 gaataatccg ccgacaccag cttgtggccc ggcgatgcaa tgaaggcgcg gcgaatcttg
     1501 cgaccgtcct cggttcgcac cggaatgttt tgtaaattcg gttcgttcga cgacagccgg
     1561 ccggtggtgg tcgccgccaa tgcgtaggtg gtgtggacgc gatgggtctg cggatgcaca
     1621 taggtcggca gcgcgtcggt gtaggtcgat ttcagttttg aaacctgccg ccactccaga
     1681 atcttcttgg gaaattcgtg gccctgctcg gcgagttcgt cgaggatctg tgcggaggtc
     1741 gaccaggcgc cggtcttggt tttgctgccg ccgggtatgc ccatcttgcc gaacaaaatg
     1801 tcgccgatct gtttcgggct gccaacattg atcggctcgc cggcgatctc ctgaagctcg
     1861 gcctcgacac gcgcggcggt ctgggcgaat tcgcctgata gccgcgacag cacctgacgg
     1921 tcgatcgaga tgccgcgccg ttccatgcgc gccagcaccg agatcagcgg ccgctccagc
     1981 gtctcgtaaa cggcggtcat gcgttcggcg acgaggcggg gttttaagac gcgccatagc
     2041 cggaggatga catcggcatc ctcggcggcg tgggcggtaa acttgtcgat agcgacttga
     2101 tcgatactga gcttgttctt gccgctgccg attacgtcgc tatcggtgag aacggcgtga
     2161 ccgagccatg tctgggccag cgcatcgagc ccgtgcgcgt tccggccggc gtcgagcgca
     2221 tacgacatca gctgtgcgtc gtcgtggttg cggatggtaa tgcccgcttg cgcgaacatc
     2281 acggcgttga atttgatgtt gaagccgatc ttcagaatcc ccgcggattc caatagcggc
     2341 cgcagcgcct cgagcgcgtc gtcagccttg atttgctcag gtgccaagcc tgcggcaaac
     2401 aggcctgagc catcgcctga ctgcttgtgg ccgaggggga cataggcggc gtcgctggcc
     2461 cccagcgcca gcgcaatgcc gcagatctcg gcctgcatcg gatcgaccga attcgccttg
     2521 gcctcgatgg cgaaatggcc cacatcatgc actcgtgcga tccaccaccg cagctcctcg
     2581 aggctgcgga tcgtctggta tttgctgcga tcgatctttg tcttgcgcgc ggcttcggcg
     2641 cgggcagtgg caagcgagat aggcgtgccc ttgttgctcc cggctttgtc ttccttgcct
     2701 ccgtcggttt tgcccggcgc gcttcctctc gcgttggcgg gggagggctt gccttcgact
     2761 tggtcacggg gcggggcact ttggcctgcg aaaaggtcgc cgtccagcgc gttgccaagt
     2821 tcgtcgacct tctcggcaag cggaagcgat gagaaaacac tcgcgccgct tttcgacccg
     2881 gcatccgctt ccacatccgc cggatcgact tgcgaatact cggcgaccct ccgggtcagg
     2941 gtggaaaatt ccatcgcctt caaaaacgcg atcagcttgc gtgcgtcggg ctcgtgcacc
     3001 gcgagttcgt cgagcggcac gtcgagcttg accttgtcgt cgagcagcac cagttgccgc
     3061 gagatccgcg ccttctcggc attctcgatc agcgcctcgc gccgcttcgg ctgcttgatc
     3121 tcgcccgcgc gtaacagcag cgtctccaga tcgccatatt cgacgatcag ctgcgcggcg
     3181 gtcttgatgc cgatgccggg cacgcccggc acgttgtcgg tcgaatcccc ggccagcgcc
     3241 tgcacctcga ccactttttc cggtggcacg ccgaatttct cgatcacctc ggcaatgccg
     3301 atgcggcggt ccttcatggt gtcgaacatg gtcaccttgt cggtgacgag ctgcatcagg
     3361 tccttgtccg aggagacgat ggtcgcggtg gcgccgcgtt cgccggcctc gcgcgcgtaa
     3421 gtcgcgatca gatcgtcggc ctcgaagccg ccctgctcga ggcagggcag gtcgaacgcg
     3481 cgcacggcct cgcggatcag cggaaattgc gggatcaggt cgtccggcgc cggcgggcgg
     3541 tgtgccttgt aatcgggata gagcttgttg cgaaaggtaa tctcggactt gtcgaagatg
     3601 atggcgagat gggtcggccg gttgtcgggt ggcatgtcgc gcaacagttt ccacagcatg
     3661 ttgcagaagc cgagcacggc attgacctgc aggccatcgg atttgcggtt cagcggcggc
     3721 agcgcgtggt aggcgcggaa aatataggag gaaccgtcga ccagaaagac gtggtcgcct
     3781 ttcgcaaggg ctttgacggc aacggctttt ggaagcgttt gttctgatgt tttcggcatg
     3841 gccgcaactt agggattttt gcggtgattg acaccctttg gggcgggatc tgccctgatt
     3901 tttgttcctc ctccgaccgt cccggaatga gagctgaggg cgcattcagc cgtcttgccc
     3961 ggacttgatc cgggcatcca tcgatcttca aacgagtcca acgaacagga tggattgccg
     4021 ggtcaagccc ggcaatgaca acaggaggcg t
//
LOCUS       DATNOI010000060         3712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2122724,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000060 DATNOI010000000
VERSION     DATNOI010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2122724"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..2960)
                     /locus_tag="VN968_02035"
     CDS             complement(249..2960)
                     /locus_tag="VN968_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXL28060.1"
                     /translation="MTANGTASFSLKHFGRGGPIRWLVFGGVFLIAAITVGATIMAGD
                     FRERALQSSERELENTVLLLARHFDQQFQDLGAIQEDFVAYVKSAGIDTSEQYRRRLS
                     GEDIHVMLKAKIGALSYVGGINLFDSDGTLINSANVWPVPAVSIADRGFFKTLKSDPR
                     SPSLLVEPVYSRITGLWTTVLARRMTGPNGEFLGTSGRGIEPANFEKFFASLALGDGA
                     AISMYHRDGTLLARHPHVDAMIGMNFKTGPVHQQVLSKSDHGTIRITSPIDGQKRLAS
                     ARILSHFPISVVATTTVAAALADWRQQIRSLIAVAGFSVLAIAALLFLAVRKLSQQHQ
                     LEKQRLDTAINNMTQGLLMFDASRRLVVANQRYIEMFGVSTEVVKPGCTLPQLLQHRK
                     ETGSFSGDVDEYCSNLYGKLAQGKVFQKILDAADGGSIQVSYRPLRRGGWVTTLEDIT
                     ERRRVEDRITHLAHYDALTDLPNRALFHEQLKRELARIVPGEQLAVLYIDIDEFKSVN
                     DTLGHLIGDQLLKSVAVSLGHCVRGTDFVARLGGDEFAIVQTAVKTVDDVADLVTRVL
                     DAIRRPYECLGHQVTTDASIGIALAPQHGADLDQILKNADLAMYAAKSAGRRTYRFFE
                     PEMDARVRARRILEMDLRQAIIDGSFEVYYQPSVTLEDNKIVGCEALLRWRHSERGMI
                     SPAEFVPVAEETGLIDELGEWVLTTACAEAATWPDDIKIAVNVSPVQFKSGTLALKIV
                     AALAASGLQASRLELEITEAVLIRDDEAALAILHQLRAIGVRIALDDFGTGYSSLSYL
                     QRFPFDKIKIDRCFVNDIAEPDGSSCIVQAVVNIAAVRHMTTTAEGVETQQQLELLRA
                     LGCAEMQGYLFSPAKPAAEIRQLFDRKPTAAVNLSPARKRLQISNPA"
     gene            complement(3123..>3712)
                     /locus_tag="VN968_02040"
     CDS             complement(3123..>3712)
                     /locus_tag="VN968_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL28061.1"
                     /translation="SVSEAIRSRRFISLYAACLICSFGVFVPFVHLVPYAQDHGVAPS
                     SAVLLLGVIGVGSTAGRFFLGGLADRLGRQFSLLLMFTGMALALAIWVVSANVWSLVA
                     FAFIYGVFYGGWVAVLPAVVMDLFGGRNVSGLIGILYTSVAFGTLIGPSAAGFAFDLS
                     RSYTLPILASAGANIFAAAIVVATSRVASANKPGS"
BASE COUNT          746 a         1133 c         1154 g          679 t
ORIGIN      
        1 ggcgcgccag ccctcgaccc taccgatgag cggggtgtga aaacggggcc atggtcgaac
       61 cagtaaggca ccgccgaacg aaaggggcgg caacagatat gctcgaacct aaagctaccg
      121 cgtcacacct cgactctaca catcgcgtca tttcgctgcg gtgcggaatt tgtttgctac
      181 cggggcatag cggaaattgg tcaagcccgc accaatcaag gctcgattta tgagtacgcg
      241 ccctggattc acgcagggtt ggaaatttgc agacgctttc gggccgggct caggttcacg
      301 gccgccgttg gtttgcggtc aaatagctgc ctgatctcgg ctgccggctt ggccggactg
      361 aacaggtagc cctgcatttc ggcgcagccg agcgcgcgaa gcagctcgag ctgctgctgc
      421 gtctcaacgc cttccgcggt tgtcgtcatg tgacgtaccg cggcgatgtt gacgaccgcc
      481 tgcacgatgc aggatgaccc gtccggctct gcgatgtcgt tgacgaagca gcggtcgatc
      541 ttgatcttgt cgaacggaaa gcgctgcaga taactcagcg aggaatagcc ggtgccgaag
      601 tcgtccagtg ctattctgac gccgatagcg cgaagctgat gcagaatggc aagcgccgcc
      661 tcgtcgtcgc gaatcagcac cgcctcggtg atctccagtt ccagccggct cgcttgcagg
      721 ccggacgccg cgagtgcggc gacgatcttc agcgccagcg tgccactctt gaactgaacc
      781 ggtgaaacgt tgaccgcgat cttgatatcg tccggccagg tcgccgcttc cgcgcaggcg
      841 gtcgtcagca cccattcgcc gagctcgtcg atcagaccgg tctcttccgc gacgggaacg
      901 aactcggcgg gagagatcat gccgcgttcg gaatgccgcc agcgcaacag cgcctcgcag
      961 ccgacgatct tgttgtcctc aagcgtgacg ctcggctggt agtagacctc gaaggagcca
     1021 tcgatgatcg cctggcgcag atccatttcc aggatgcggc gtgctcttac ccgcgcgtcc
     1081 atctcgggct cgaagaagcg atacgtccgg cgtccggcgg atttggcggc atacatcgcc
     1141 agatcggcat tcttcagaat ttgatcgaga tcggcgccgt gctgcggagc cagcgcaatg
     1201 ccgatgctgg catcggtcgt cacctgatga ccgagacatt catagggcct gcggatcgca
     1261 tcgaggaccc gcgtcaccag atcggcgacg tcatcgacag tcttgaccgc ggtctggacg
     1321 atggcgaatt catcgccacc cagtcgtgcg acgaagtcgg tgcctctgac gcaatggccc
     1381 aggctcacgg cgacggattt cagcaattga tcgccgatca ggtgcccgag cgtatcgttg
     1441 acgcttttga attcgtcgat gtcgatgtag agcaccgcca attgctcgcc cggaacgatc
     1501 cgagccagct cacgcttcaa ctgttcgtgg aacaacgccc ggttcggcag gtcggtcagc
     1561 gcatcgtagt gcgcaaggtg cgtgatccgg tcctcgacgc gcctgcgctc ggtgatatcc
     1621 tccagggtcg tcacccatcc accacggcgt agcggccggt acgagacctg gatcgagccg
     1681 ccatcggcgg cgtccaaaat cttttgaaag acctttcctt gcgccaattt gccgtagaga
     1741 ttggagcaat attcgtccac gtcaccagag aacgatccag tttccttccg atgctggagc
     1801 agttgcggca gggtgcagcc cggcttcacc acctcggtgg acacgccgaa catctcgata
     1861 tagcgctggt tggcgacgac gagccggcgc gatgcgtcga acatcagcag gccttgcgtc
     1921 atgttgttga tggcggtgtc gaggcgctgc ttttccaatt gatgctgctg cgacagcttg
     1981 cgaaccgcga gaaacagcag ggcggcgatc gcgagcaccg aaaatccggc caccgcgatc
     2041 agggatctga tttgctgccg ccaatcggcc agcgccgccg cgaccgtcgt cgtcgcgacg
     2101 accgaaatcg gaaaatggct caatatgcgg gccgaggcca gccgtttctg gccgtcgatg
     2161 gggctggtta tccgtatcgt gccgtggtcc gatttcgaca agacttgctg atgaaccgga
     2221 ccggttttga aattcatccc gatcatcgca tcgacgtgcg gatgacgcgc cagcagcgtg
     2281 ccgtcgcgat ggtacattga aatcgccgcg ccatctccaa gcgccaggct ggcgaagaat
     2341 ttttcgaagt tggccggctc gatgcctctt ccgctggtac cgaggaactc accgttcggt
     2401 ccggtcatcc tgcgggcgag gacggtggtc cagaggccgg tgatacggct gtacaccggt
     2461 tcgaccaaaa gcgatggtga ccggggatcc gatttgaggg ttttgaaaaa gcctcgatcg
     2521 gcgatgctga cggccgggac cggccagacg ttcgccgagt tgatcagtgt tccgtcggaa
     2581 tcgaacaggt tgatgccgcc gacataggac agcgcaccga tcttggcctt gagcatcacg
     2641 tgaatgtcct cgccggacag ccgacgccgg tattgctcgc tggtgtcgat tccggccgat
     2701 ttcacgtaag cgacaaagtc ttcttgaatc gccccgagat cctggaattg ctggtcgaaa
     2761 tgacgggcca gcagcagcac ggtgttttcc agttcgcgtt cgctgctttg cagcgcgcgc
     2821 tcgcggaaat caccggccat gatcgtcgcg ccgaccgtga ttgccgcgat caggaatacg
     2881 ccgccaaaga ccagccagcg gatcggaccg ccgcgaccga aatgtttcaa gctgaaggag
     2941 gcggtcccgt ttgccgtcat ggtcttgatg ctgccaggcc tgaatgccag aggcagtgtc
     3001 gcgcgccccc aacccgcaac agtagtaccg cgccgaaatg gaggtggcgt taggaatcta
     3061 ggttaatgaa gggtcaacag gcgtcgtccg gccgcggcgg agcgagccaa gttgcaatta
     3121 tcttagctgc ccggcttgtt cgcagatgcc accctcgacg tcgccaccac gatcgccgca
     3181 gcgaagatgt tggcgcccgc gctggccagg atcggcagcg tgtaactgcg gctgaggtcg
     3241 aaggcgaagc ccgcagcgct cggcccgatc agggtgccga acgccacgct ggtgtagagg
     3301 atgccgatga ggccgctgac attgcggccg ccgaaaaggt ccatcaccac ggccggaagc
     3361 accgcgaccc atccgccgta gaacacgccg tagatgaacg caaaggcgac cagcgaccag
     3421 acgttcgccg agaccaccca aattgccaat gcgagcgcca tgcccgtgaa catcaacagc
     3481 agggaaaact ggcggccgag cctgtccgca aggccgccga ggaagaaccg cccggcggtc
     3541 gaaccgacgc cgatcacgcc cagcagcagc acggccgacg agggcgccac gccgtgatct
     3601 tgcgcatagg ggacgaggtg aacgaacggc acgaacacgc cgaacgaaca gatcaagcaa
     3661 gcggcgtaga gactgatgaa gcggcgtgac cggatcgctt cgctcacgga ag
//
LOCUS       DATNOI010000061         3317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1034873,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000061 DATNOI010000000
VERSION     DATNOI010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034873"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VN968_02045"
     CDS             <1..272
                     /locus_tag="VN968_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXL28062.1"
                     /translation="EILADDFYCSNPDKSLVDRAGFLKQTAVPVTIKNLEAHDVKIRV
                     MGDFSIIHARTSYTTADGQQASGRYTDCWARQDGRWLAVSAHVSR"
     gene            complement(380..1468)
                     /locus_tag="VN968_02050"
     CDS             complement(380..1468)
                     /locus_tag="VN968_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HXL28063.1"
                     /translation="MKAAVLHEVNTPLVIEDVSLPKPGPREVLIRTSVAGLCHSDLHF
                     MEGLYPHPLPAVLGHESAGVVEQVGSDVNYVKPGDHVVTCLSVFCGTCDNCTTGRTVL
                     CTDTTVKMLPGASSRLSWARSEKLNQFLNLSSFAEQMLVHENAIVKIRKDMPLELAAL
                     IGCGVITGYGAVVNTAKVAPGETVVVIGCGGVGMAAINGAQIAGAGRIIAVDTNPAKL
                     QLATKLGATDIVDPQNGDVVQQVRDLTGGGVHHSFEVLGRKETAEQAFAMLAPGGTAT
                     IVGMIPFGQKIELHGFDFLRERKIQGSSMGSNHFRVDMPRLIEFYMRGKLHLEDWISA
                     KLKLSEINEGFAAMKAGKTVRSVIMFDV"
     gene            complement(1653..>3317)
                     /locus_tag="VN968_02055"
     CDS             complement(1653..>3317)
                     /locus_tag="VN968_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL28064.1"
                     /translation="RIHLPDALFRHIEPHLDRLGALAGGHLDECARLADRHVPVLHQR
                     DRFGRDAQWIEYHPAYHELERAAFGEFGIHAMSLRKGILGWPEQYPVVAKHAFTFLFN
                     QAEFGLGCPINVTDGCAKLLSRFGDEALKAKYLDGLTQTDMNKLTQGGQFMTEKEGGS
                     DVGTLTTSAVQDGDRWILSGEKWFCSNADAEVVMLLARPQGAVGGTRGVGLFLMPRWL
                     DDGSPNRYRIVRLKDKLGTRSMASGEIKLEGAIAYAVGKLDRGFVQMAEMVNSSRLSN
                     GVKSTALMRRGHHDAMTVARNRVVFGSRIIDLPLARRQLMKIMLATEQALSMSFLTAD
                     ALDRAEAGSQDAAALLRILTPTLKFRATRDARKVCGDALEMRGGIGYIEEFATARLLR
                     DAHLGSIWEGTGNIVALDALKRAVGRHGADAALAADLHARLDDSANVPPAWRDRLREL
                     TDRAVGFAREVAGRIDNEAEARRATSLLYHVASAVALAWEGGRIHAMRGDARRLLLSR
                     MVIDHRVFGNDPFRLAENAAQRAIAGHLLGMDAVGMAEVGELLVAA"
BASE COUNT          610 a         1070 c         1023 g          614 t
ORIGIN      
        1 atgagattct tgccgacgac ttctattgct ccaatcccga caagtcgctg gtcgaccgcg
       61 ccggctttct caagcagacc gcggtgcccg tgacgatcaa gaatctcgaa gcccatgacg
      121 tcaagattcg cgtgatgggc gacttctcga tcatccacgc ccgcaccagt tatacgaccg
      181 cggacggtca gcaggcctca gggcgttaca ccgattgctg ggcccggcaa gatggccgct
      241 ggctcgcggt gtcggcgcat gtttcaagat aatgaccgtc aacgccgggc ttgacccggc
      301 aatccatccc tttgggaaaa attcttgcga agaagatgga tgtgcggatc aagtccgcgc
      361 atgacgaccg tgtgcgcggt cacacatcaa acatgatcac gctgcgcacg gtcttgccgg
      421 ccttcatcgc ggcaaagcct tcgttgatct cggacagttt cagcttggcc gaaatccagt
      481 cctccagatg aagcttgccg cgcatgtaga attcgatgag ccgcggcatg tcgacgcgaa
      541 aatggttcga gcccatcgac gagccctgga tcttgcgttc gcgcaagaag tcaaacccgt
      601 gcagttcgat cttctggccg aacggaatca tgccgacaat ggtcgcggtg ccgccgggcg
      661 ccagcatcgc gaacgcctgc tccgcggttt ccttgcggcc cagcacttcg aacgagtgat
      721 gcacgccgcc acccgtaaga tcgcgcacct gctgcaccac gtcgccgttt tgggggtcga
      781 cgatgtcggt ggcgccgagt ttggtcgcca gttgaagctt ggccggattg gtgtcgaccg
      841 cgatgatgcg gccggcgccg gcgatctgcg cgccgttgat cgcggccatg ccgacgccgc
      901 cgcagccgat caccaccaca gtctcgccgg gcgcaacctt cgcggtgttc accacggcac
      961 cgtagccggt gatgacgccg cagccgatca gcgccgccaa ttcgagcggc atgtccttgc
     1021 ggattttgac aatggcgttt tcgtgcacga gcatctgctc ggcgaacgac gacaggttga
     1081 gaaactgatt gagtttctcc gatcgcgccc acgacagccg gctggatgcg ccgggcagca
     1141 tttttacggt ggtgtcggtg cacagcaccg tgcggccggt ggtgcagttg tcgcaggtgc
     1201 cgcagaacac cgacagacaa gtcacgacat gatcgccggg cttcacgtag ttgacgtcgg
     1261 agccgacctg ctcgacgacg ccagcggatt cgtggcccag caccgcgggc agcggatgcg
     1321 gatagagccc ttccatgaaa tgcaggtcgg aatgacagag cccggcgacg gaagtgcgga
     1381 tcaagacctc gcgcggtccg ggttttggca ggctgacgtc ctcgatcacg agcggcgtgt
     1441 tgacttcatg caggacggcg gccttcatcg gtgtttcctc ttttgtttgc tttgttttgt
     1501 tttgctttgt tttgttttgt tcttaaaact tgggaatcgc gctccctctc cccttgaggg
     1561 gttacggcct atcgatggag cgtaacccct cacccggatc gcaagtgcga tcgacctctc
     1621 ccacaagggg gaggtaacat cacgcggcga gcctacgctg ccacgagcaa ttcgccaacc
     1681 tcggccatgc cgacggcgtc catgccgagc agatggcccg cgatggcgcg ttgcgcagca
     1741 ttttccgcga gccggaatgg atcgttccca aagacccggt gatcgatcac catgcgcgac
     1801 agcaacagcc ggcgggcatc gccgcgcatc gcgtggatgc gcccgccctc ccaggccagc
     1861 gccacggcgc ttgcgacatg ataaagcagg ctggtggcac gccgcgcttc agcttcgttg
     1921 tcaatgcgcc cggcgacctc gcgggcaaag ccgacggcgc gatcggtcaa ttcgcgcaac
     1981 cggtcgcgcc aggccggcgg cacattggcg ctgtcgtcga ggcgagcgtg caggtcggcg
     2041 gcgagtgctg cgtcggcgcc atggcggccg accgcgcgct tgagcgcatc gagcgcgacg
     2101 atgttgccag tgccctccca gatcgaaccg agatgcgcat cccgcaatag ccgcgcggtg
     2161 gcgaattcct cgatgtagcc gatgccgccg cgcatctcca gcgcgtcgcc gcaaaccttt
     2221 cgggcatcgc gggtggcgcg gaatttcagg gtcggcgtca gaatacgcaa tagcgccgcc
     2281 gcgtcctggc tgccggcctc ggcgcgatcg agcgcgtcgg cggtcaagaa actcatcgac
     2341 agcgcctgct cggtggccag catgatcttc atcaattgcc gccgcgctag cggcagatcg
     2401 atgatgcggc ttccgaacac cacacgattt cgcgccaccg tcatcgcgtc gtgatgcccg
     2461 cgccgcatca gcgcagtcga tttgacgccg ttggagagcc gcgacgaatt gaccatttcg
     2521 gccatctgca caaagccgcg atcgagcttt ccgaccgcat acgcaatcgc gccctccagc
     2581 ttgatctcgc ccgacgccat cgagcgggtg ccgagcttgt ccttcaggcg aacgatccga
     2641 tagcgattgg gcgagccatc gtcgagccag cgcggcatca gaaacaggcc gacgccgcgg
     2701 gtgccgccga cggccccttg cggccgcgcc agcagcatca cgacttcggc gtctgcattg
     2761 gagcagaacc atttttcgcc cgacagaatc cagcgatcgc cgtcctgcac cgctgacgtc
     2821 gtgagcgtgc cgacgtcgga gccgccttcc ttctcggtca tgaactggcc gccctgggtc
     2881 agcttgttca tgtcggtctg cgtgagaccg tcgagatatt tcgccttcaa cgcctcgtcg
     2941 ccgaagcgcg acagcagctt ggcgcagcca tcggtgacat tgatcgggca acccagcccg
     3001 aactcggcct ggttgaacag gaatgtaaag gcgtgcttgg ccacgacagg atattgctcc
     3061 ggccagccca gaataccctt gcgaagcgac atcgcatgaa tgccgaattc accgaacgcg
     3121 gcacgctcca actcgtgata ggccggatgg tactcgatcc actgcgcgtc gcggccaaaa
     3181 cggtcgcgct ggtgcagcac cggcacatgg cgatcggcca gccgcgcgca ttcgtcaaga
     3241 tgaccgccgg cgagcgcgcc cagccggtcg agatgcggct cgatatggcg aaacagcgca
     3301 tcgggcagat gaatgcg
//
LOCUS       DATNOI010000062         6594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1195252,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000062 DATNOI010000000
VERSION     DATNOI010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195252"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VN968_02060"
     CDS             complement(<1..702)
                     /locus_tag="VN968_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXL28065.1"
                     /translation="MNSVAYAEIGITTNFSFLRGGSHPQDYVHEASKLRLPAIGIADH
                     NTLAGVVRAYKELDNSELTYKPKLLIGSRLVFMDGTPDILVYPRDRAAYGRLCQLLTH
                     GKRGDDTEKGECHLGFENLLEFAEGQLLAVMLPHRLNAAAALDILDQLQCSRADGVWL
                     AASLLYRGDDRRRLMRLYRIATTAGVPLLATNEVLYHHPARRPLQDVLTCIREKTTID
                     AIGRRLEANAERYLKP"
     gene            complement(699..2288)
                     /locus_tag="VN968_02065"
     CDS             complement(699..2288)
                     /locus_tag="VN968_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HXL28066.1"
                     /translation="MSACSQNRRRFLSLWLPRLPIDRIKRQLARGAPVNDEPSVVVTK
                     QNNALQIFALDDAAADLGLEIGLPLANARAICPHLTVFDADAAADAKALNDIADWCDR
                     FTPLVALDSPHGLFLDITGCAHLFGGEAVMMRLLCDVLTAQGFAVSAAIAGTSVCART
                     MTRHVHGRIVRDGEEADAVRPLQVSALGTEDAITRGLRRAGLKTIGDVACRERHEITA
                     RFGAGFTTLLEHALGQGDAPISPRKPLPDYIVEKRFAEPIATEGVISVTLSRLAEMLV
                     GAMGQQGKGARRLEAYFFRTDGAVRAIAVDTGQPVTRADMIDRLFRERLEALNDPLDP
                     GFGFDLIRLSASRTEIVVQQQRDLDANVHDNDELAALIDRIAARIGGQRVVVHLPQDT
                     HIPERAVQAVSAQHHLAIAAQAAWPARVDEEPPLRPLRLFERPEPIKVPFATVPDGPP
                     HHFTWRRATHAVVRVEGPERIAMEWWRHNGQALTRDYFRVEDEAGLRFWIYRDGLYGS
                     ELVTGEGEPAPSNWFVHGLFA"
     gene            complement(2185..2976)
                     /locus_tag="VN968_02070"
     CDS             complement(2185..2976)
                     /locus_tag="VN968_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HXL28067.1"
                     /translation="MSGARTNTLAILRGSIERIESHGDTYRPDRVALGHADADAMLQG
                     GLALGAMHEVFAEGRHSAAATGFVTGLMGRAAARRPLVWIRQDFAEIESGALSMSGLA
                     ELGLDPRLLVTVRAADVDTALKTAADALACDALGVVVLEVWGEARQLDLVASRKLTLA
                     AQMSGVTGLVLRVAAEPLPSTAETRWIVRAAHSPPSAQPGSAWGAPVFDAQLVRNRHG
                     PVGRWIMEWKCDECLFAKPAAVPQPVAAAPAHRPYQTPARARRAS"
     gene            3242..3838
                     /locus_tag="VN968_02075"
                     /pseudo
     CDS             3242..3838
                     /locus_tag="VN968_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687447.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision.; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
     gene            complement(4068..4565)
                     /locus_tag="VN968_02080"
     CDS             complement(4068..4565)
                     /locus_tag="VN968_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HXL28068.1"
                     /translation="MTAKDKHLNDLFLDTLKDIYFAEKQILRALPKMAKAASSDKLRA
                     AFEKHHGETEGQVERLEQIFELLDKPARGKTCDAIQGILDEGKEIMDEYKGSEALDAG
                     MLAAAQAVEHYEISRYGTLKQWATQLGMKDAVKLLDQTLQEEKKTDESLTTLAEAAVN
                     LAAAA"
     gene            complement(4624..4770)
                     /locus_tag="VN968_02085"
     CDS             complement(4624..4770)
                     /locus_tag="VN968_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28069.1"
                     /translation="MTEVFKAWMTEAELSEVGWLADRLYDGELIRLKPLRLAFEMTGT
                     LLMR"
     gene            4803..6431
                     /locus_tag="VN968_02090"
     CDS             4803..6431
                     /locus_tag="VN968_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HXL28070.1"
                     /translation="MTDFALNQTTDEGAPVQPAARPNLDKGFNPLTLIVFFGILAAGL
                     LFVAYSIYADVDATGVKVTTYLPYLLLLVALLIALGFEFVNGFHDTANAVATVIYTHS
                     LPAEFAVMWSGFFNFLGVLLSSGAVAFGIVSLLPVELILQVGSSAGFAMVFALLIAAI
                     IWNLGTWYFGLPASSSHTLIGSIIGVGIANALMRGRDGTSGVDWSKATEIGYALLLSP
                     LVGFIFAALLLLLLKVIVRNPALYAAPEGNKAPPLWIRGLLILTCTGVSFAHGSNDGQ
                     KGMGLIMLILIGTVPTAYALNRALPESQVALFQQTSAAASTVIAAKGAGHSIIGDPRP
                     AVTQYVAQHTLSEGTYPSLAVLVRDVGDQVQKYGSLSKVPSEAVGNTRNDMYLTSEAI
                     RFLMKDKQNDLNKEEIGILNAYKGSLDAATKFIPNWVKVAVAIALGLGTMIGWKRIVI
                     TVGEKIGKTHLTYAQGASAELVAAATIGAADVFGLPVSTTHVLSSGVAGTMAANGSGL
                     QLATIRNLLMAWVLTLPAAIMLSAGLYVLFSNIF"
BASE COUNT         1232 a         2126 c         2008 g         1228 t
ORIGIN      
        1 gggcttgagg tagcgttccg cattggcttc aagacgcctg ccgatggcat cgatggtggt
       61 cttctcgcga atgcaggtca gaacatcctg caggggacgg cgcgcgggat ggtgatacag
      121 cacctcgttg gtcgccagca gcggcacgcc ggccgtcgtg gcgatacgat agagccgcat
      181 cagacggcgc ctgtcgtcgc cacggtacaa caggctggcc gcaagccaca ccccatcggc
      241 gcggctgcat tgcaactggt ccagaatatc aagcgctgct gccgcgttga ggcgatgcgg
      301 cagcatcacg gccagcaact ggccttcggc gaattccagc aggttttcga atcccaaatg
      361 gcattcgccc ttctcggtat cgtcgcctcg cttgccgtgg gtcagcaact ggcatagccg
      421 gccataggcg gcgcggtcgc gtggatagac cagaatgtcc ggcgtgccat ccatgaagac
      481 gaggcgcgaa ccgatcagaa gctttggctt gtatgtcagc tctgaattat cgagctcctt
      541 gtaagcccgc accacgccgg ccagcgtgtt atggtcggcg atgccgatgg cgggaagccg
      601 cagcttgctc gcctcatgca cgtagtcctg cggatgcgag ccgccgcgca ggaacgaaaa
      661 attcgtcgtg attccgattt cggcgtaagc gacactattc atgcgaacag cccatgcacg
      721 aaccagttgg aaggcgccgg ctcgccttcc ccggtgacaa gttcgctgcc gtaaagtccg
      781 tcccggtaaa tccagaaccg caatccagcc tcatcctcga cgcggaaata atcccgcgtc
      841 agcgcctgac cgttgtgccg ccaccattcc atggcgatgc gctcggggcc ctctacccgc
      901 accaccgcat gggtcgcccg ccgccatgta aaatggtgcg gcggcccgtc agggaccgtc
      961 gcaaacggca ccttgatggg ctccggtctt tcgaacagcc gcaacggccg caacggcggc
     1021 tcctcgtcca cccgcgccgg ccacgccgcc tgcgcggcta tcgccagatg atgctgcgcc
     1081 gatacagcct gcaccgcgcg ctccggaata tgggtgtcct gcggcaggtg cacaaccacg
     1141 cgctgtccgc cgatccgcgc ggcgatgcgg tcgatcaggg cggccaattc gtcattgtcg
     1201 tgcacattgg cgtcgagatc gcgctgctgc tgcacgacga tttcggtgcg gctggcggac
     1261 aggcgaatga gatcaaaacc gaagccggga tcgagcggat cgttcaaggc ctcgagccgc
     1321 tcgcgaaaca ggcggtcgat catgtcagct ctggtcacgg gctgtccggt atcgaccgcg
     1381 atcgcgcgca ccgcgccatc ggtgcgaaag aaataagctt ccagccgccg cgcgcccttg
     1441 ccttgctggc ccatcgcccc gaccagcatc tcggccagcc gggataatgt caccgatatc
     1501 acgccctcgg tggcgatcgg ttcggcgaac cgcttctcca cgatgtaatc cggcagcggt
     1561 tttcgagggc tgatcggggc atcgccctgc ccgagcgcgt gttccagtag agtcgtaaac
     1621 ccggcgccga accttgcggt gatttcatga cgttcacggc aagcgacatc gccgatggtt
     1681 ttcagcccgg cgcggcgcag gccgcgggtg atggcatcct cggtgccgag cgccgacacc
     1741 tgcagcggcc tgaccgcatc ggcttcctcg ccatcccgga cgatgcggcc atggacatga
     1801 cgcgtcatcg tccgcgcgca caccgaggtg ccggcgatgg ccgcactgac ggcaaagcct
     1861 tgcgcggtca acacgtcgca caacagccgc atcatcacgg cctcgccgcc gaacagatgg
     1921 gcgcagccgg tgatgtctaa aaacaggcca tgcggggaat ccagcgctac cagtggcgta
     1981 aagcggtcgc accagtcggc gatgtcattc agcgccttgg cgtcggccgc tgcatcggcg
     2041 tcgaacaccg ttagatgcgg acagatggcg cgggcattgg ccaagggcaa gccgatctca
     2101 aggccgagat cggcggcggc atcatccagc gcaaaaatct gcagcgcgtt gttttgcttg
     2161 gtgacgacga cgctgggttc atcattaact ggcgcgccgc gcgcgagctg gcgtttgata
     2221 cggtcgatgg gcaggcgcgg cagccacagg ctgaggaacc gccgccggtt ttgcgaacag
     2281 gcactcatca catttccatt ccatgatcca ccgcccgaca gggccatggc ggttgcggac
     2341 aagctgcgcg tcgaacaccg gtgcgcccca cgcgcttccg ggctgtgcgg agggcggcga
     2401 atgcgccgcg cgcacgatcc atctggtttc cgcggtcgag ggcaatggct ccgccgccac
     2461 ccgcagcacc aggccggtca cgccggacat ctgcgcggcc agcgtcagct tgcggctggc
     2521 gacgagatcc agttgtcgcg cctcgcccca gacttccagc accacgacgc cgagcgcatc
     2581 gcaggccagc gcgtcggccg ccgtcttcaa cgcggtgtcg acatccgccg cgcgcaccgt
     2641 caccagcagg cgcggatcga gcccgagttc ggccagcccg ctcatcgaca gcgcaccgga
     2701 ttcgatttcg gcaaaatcct ggcggatcca caccagcggc cgccgtgccg ccgcccgtcc
     2761 catcaggccc gtgacaaaac ccgtcgcggc ggcgctgtgc cgtccttcgg cgaacacctc
     2821 gtgcatcgcg cccagcgcga ggccgccctg cagcatcgca tccgcgtcgg catggccgag
     2881 cgccaccctg tcaggcctgt acgtatcgcc gtgcgactcg atgcgctcga tgctgccgcg
     2941 caaaatcgca agcgtgttcg tgcgtgcgcc gctcatgcgc cgctcctcaa gatgttgggc
     3001 cgtcacttga ttcagagaga accagaaact ggctcaattg ttcatgatat gttctaatat
     3061 aaagctaaga tggccaagag agtcaatcgg gattgatggc tatgtttttc gtgagcaatc
     3121 tcaatgggat cgaaggaacc ttcaacgccc tgcctgcatt gaagcctggc tcgcggccct
     3181 gatcgcaacg gcaactggaa aaaccgccat gacaactacg ttacacatgt ccaccggcag
     3241 cctcgacacc ctgcgcgacg aagccgccaa ttgccgggcc tgtccgcttt ggaaagatgc
     3301 gacccagacc gtgttcggcg aaggcccgca agctgcgcaa gtgatgctgg tcggcgagca
     3361 gcccggcgac aaggaggatc tggccgggaa acccttcgtc ggaccggcgg ggcagatgct
     3421 cgaccgcgcg ctggaagagg ccggcatcga tcgcaagaaa gtttacgtca ccaatgcggt
     3481 gaaacatttc aagttcgtac cgcgcggaaa aatccgcctg caccagaagc cgacgacgcc
     3541 ggagatcaag gcgtgccggc aatggtacga acacgaactc aaggcgatca agcctgatct
     3601 ggtggtggcg atgggcgcca ctgctgcgca aagcgtgttc ggaaaaatca cgccgatcaa
     3661 caaaaatcgc gggcgcctga tcgatctcga tgatggcatc aaggcgctgg taacggtgca
     3721 tccgtcctat ctgttgcggc tgcccgatgc cgacgccaag gcgcgcgaat acgaacgttt
     3781 tgtcgaggat ctgaaaattg ccgcgaagct gctgaggaag tcagcgcgtg cggcctagat
     3841 tagtataaaa agcgaatgca gaactcttct tccctctccc cttgcgggag agggtggctt
     3901 cgcgaagcga agacgggtga ggggttccgg cctatcgatt cgaagaggac ccctcatccg
     3961 gcacggactt cgcatagccg atgcaaagca tcggcgtcct cttaaaggac ggcgcccgaa
     4021 ggccgcctat ggccaccttc tcccacaagg ggagaaggga agaaaaatca agccgcagcg
     4081 gcgaggttga ccgcggcttc cgcgagggtc gtcaggctct cgtcggtctt cttttcctcc
     4141 tgcagggtct ggtcgagcag cttgaccgcg tccttcatgc cgagctgcgt ggcccattgc
     4201 ttcagcgtgc cgtagcggga gatttcgtaa tgctcgacgg cctgggctgc ggcaagcatg
     4261 ccggcgtcga gcgcctcgct gcccttgtac tcgtccatga tctccttgcc ctcgtcgagg
     4321 atgccctgga tcgcgtcgca ggtcttgccg cgcgccggct tgtcgagcaa ttcgaagatc
     4381 tgctcaaggc gctccacctg tccttcggtc tcgccgtgat gcttctcgaa cgccgcccgc
     4441 agcttgtcgg aactggccgc cttcgccatc ttcggcagcg ctctcaaaat ctgcttttcg
     4501 gcaaaataaa tgtccttcag ggtatcgaga aagaggtcgt tgaggtgttt gtctttcgcg
     4561 gtcatggcag tactccaata atgagacgta agtgagatac gggatgtccc cgcctctcca
     4621 acgtcacctc atgagcaatg ttccagtcat ttcaaaagca agccgcagcg gcttcagccg
     4681 aatgagctcg ccgtcgtaaa gtcgatcggc cagccagccg acttcggata attccgcctc
     4741 tgtcatccag gctttaaaaa cctcggtcaa agattaaagc tcacaccgtc gaggaggtta
     4801 gcatgaccga ttttgcactc aatcagacga cggacgaagg ggcgccggtc cagccggctg
     4861 ccaggcccaa tctcgacaag ggcttcaatc ccctgacctt gatcgtgttc ttcggcattc
     4921 tcgcggccgg gctgttgttc gtcgcctaca gcatctacgc cgacgtcgac gcgaccggcg
     4981 tcaaggtgac gacctacctg ccctatttgc tgctgctggt ggcgctcctg atcgcgctcg
     5041 gctttgaatt cgtcaacggc ttccacgata ccgccaacgc ggtcgcgacc gtgatctaca
     5101 cccattcgct gccggcggaa ttcgcggtga tgtggtcggg gttcttcaac ttcctcggcg
     5161 tgctgctctc cagcggcgcg gtcgccttcg gtatcgtgtc gctgttgccg gtcgaactga
     5221 tcctgcaggt cgggagcagc gccggctttg cgatggtgtt cgcgctgttg atcgcggcga
     5281 tcatctggaa tctcggaacc tggtatttcg gcctgccggc ctccagttcg cacaccctga
     5341 tcggttccat catcggcgtc ggcattgcca atgcgctgat gcgcggccgc gacggcacct
     5401 ccggggtgga ctggagcaag gccaccgaaa tcggctacgc gctgctgctg tcgccgctgg
     5461 tcggctttat cttcgcagca ctgctgctgc ttctgctcaa ggtgatcgtc cgcaatccgg
     5521 cgctctatgc ggcgccggaa ggtaacaagg cgccgccgct ctggatccgg ggcctgttga
     5581 tcctgacctg caccggcgtc agctttgcac atggttcgaa cgacggccag aagggcatgg
     5641 gcctgatcat gctgatcctg atcggcaccg tgccgacggc ctacgcgttg aaccgggcgc
     5701 tgccggaatc gcaggtggcg ctgttccagc agacctcggc cgcagcctcc accgtgatcg
     5761 ccgccaaggg cgccggtcac agcattatcg gcgatccacg cccggcggtg acccaatatg
     5821 tcgcccagca cacgctcagc gagggcactt atccctcgct tgcggttctg gtgagggatg
     5881 tcggcgacca ggtgcagaaa tacggctcgc tcagcaaggt tccgtcggag gcggtcggca
     5941 atacccgcaa cgacatgtac ctgacgtcgg aggcgatccg gtttctgatg aaagacaagc
     6001 aaaacgatct gaacaaggaa gaaatcggca tcctgaacgc ctacaaaggc tcgctcgatg
     6061 ccgccaccaa gttcattccg aactgggtca aggtcgcggt cgcgatcgcg cttggccttg
     6121 gcaccatgat cggctggaag cgcatcgtga tcaccgtcgg cgagaagatc ggcaagaccc
     6181 acctcaccta cgcgcaaggc gcctccgccg agctcgttgc cgccgcgacc atcggcgccg
     6241 ccgacgtgtt cggcctgccg gtctcgacca cgcatgtgct gtcgtcaggc gttgccggca
     6301 cgatggcggc gaacggatcg gggctgcagc ttgcgaccat ccgcaacctg ctgatggcct
     6361 gggtgctgac gctgccggcc gcgatcatgc tgtcggccgg cctctatgtg ctgttctcga
     6421 acatcttcta aggcaccagt actgcgtcac ctgagacgaa aaggcccgcg caagttgcgg
     6481 gcctttttgc atggccgcac cggtcgctcc ctcccatcac tccgtccagg ccggaatagt
     6541 cctgcgaatt ccggcggttt gggcgtatag ctgacggaca actccaaaga attg
//
LOCUS       DATNOI010000063        20479 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1745055,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000063 DATNOI010000000
VERSION     DATNOI010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 20479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20479
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745055"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VN968_02095"
     CDS             <1..978
                     /locus_tag="VN968_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28071.1"
                     /translation="AKWASVMRAPLIAIVLCSVGGAVCAQPADEASKMLDTCFQLART
                     ADAMCSNPKNGEAERLDCRQKASMAQLECLERVFPGRTAESAPPEMPARTVSPEMPTV
                     TASPEVPAGSVSPERPIGTLAKGEPASSQKTRARTAPAETPVATVSPEITTGTASPGV
                     PARHVDIPAMPQDTHWIVSETTSPVDYSPLVTAVIRSTTSVQDAPNTLVIRCLGPRTE
                     VLLRTQGTWRASRSNTVQVDYQIDDQPLARLQWITSADGKTASYGTDAVGFLQSLPED
                     ARLKISVLDRAGPGHEATFQLAGLDAVRKRIAVPCKWATAADKISSGKR"
     gene            complement(1036..1581)
                     /locus_tag="VN968_02100"
     CDS             complement(1036..1581)
                     /locus_tag="VN968_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28072.1"
                     /translation="MRKLFRQVTIVAFTSLSLISASLADDTKVQRSTKAHPAKSAHHS
                     AGSAAKTKAQHATKAHRLVIQVDQNDPAVMNLALNNATNVIDYYRAKGEDVDVDVITY
                     GPGLHMLRADTSPVQDRIKSLKDHAFPSKVQFSACGNTKEGMEKKEGHPITVLSEAVI
                     VPSGVVRLMELQEKGWSYVRP"
     gene            complement(1973..2908)
                     /locus_tag="VN968_02105"
     CDS             complement(1973..2908)
                     /locus_tag="VN968_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXL28073.1"
                     /translation="MSASDHTSSAAPFGNWLANQPYLLLSITALCWAGNAIVGRLAAG
                     HIPPVTLSFLRWSLAFLVILPFAWTHLKRDLGAIRSHLGTMIFLSVIGISAFNTLQYW
                     SLEHTTALNTLLLQSAGPLFVAVWSLILLGIRLTLAQAAGIAVSLTGVLVILLHGDLT
                     ALTSIAFNKGDIIFTMALAIFGLYSVLSLKRPAIHGLSFVAFTFGCGAVCLVPLWIWE
                     LFARPVMRLDAANLLSLFYVAVFPSTLAYLCFNRGVQLIGANRAAPFFHVVPVFGSAM
                     AIVFLGERPQLFHIIGFALVLTGVFVASRKPAKAH"
     gene            3064..5196
                     /locus_tag="VN968_02110"
     CDS             3064..5196
                     /locus_tag="VN968_02110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HXL28074.1"
                     /translation="MARVRLWLGSDQHRLEIAGAIVYPAAMLQKFILALAIGAGCAGV
                     AMAEPRSGIDISAIDPSVRPQNDFWQFANGKWLAATSIPADRAAWDTFSALRETNQQQ
                     LREVIEAIDPRSPDGGEPRKLADLYGSFMDEARVEAAGLDGLRDELRRIHALSDKAAL
                     PELFAHLSRLSVRIPWGLEIGPDERDATRYVAHLEQSRLGLPDRDYYLKDDAHFQAIR
                     TGYRDHIVKLLALAGEPAAETSAGEIIALENRLARLQWTRVENRDPLKTYNKRDIAAL
                     PALTTKDDWPSFLSAGGVGTEIRSIVVAQPSYFDGVGEIVREVPLATWQAYLAYNLLS
                     TYAPYLSAPFVAEEFAFEQHTLRGVPEIQPRWKRAVALVDRLTGFALGRLYVERYFPP
                     ANKARADAMIANLTAAYRESITTLDWMGPETRREALAKLKAIVPKIAYPTTWRDYSGL
                     VIRRDDLVGNVMRARRFNYDFWLAKLGHEFDRAEWLTTPQTVNAYYSASRNEIAFPAG
                     ILQPPFFDAEADDAANYGAIGSVIGHEISHAFDDHGSRFDGDGNLRNWWTEDDRTRFE
                     GKTRSLVAQYDAFSPLPGHHVNGALTLGENVADNAGLAIAERAYRLALGGCPAPDLDG
                     YTGEQRLFISFAQIWRDKVRDAARIERLKVDPHSPGQFRADGTLRNQSAFAEAFAVKP
                     GDAMYLPPEQRISLWQTDSEVVCKEKAR"
     gene            complement(5335..5739)
                     /locus_tag="VN968_02115"
     CDS             complement(5335..5739)
                     /locus_tag="VN968_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL28075.1"
                     /translation="MTGGATDGHVDQVVKARWLAIGLSQADLAEVLGGTLQLTSEDGN
                     GSNYVDADRLMRVAEALQIPIDFFYDQTIGTEREAPDVSSAQRINSLQSVLALRLLRA
                     FQDMTDLRTKEVLILLAEQIVKCQASRGGDGG"
     gene            complement(5736..6770)
                     /locus_tag="VN968_02120"
     CDS             complement(5736..6770)
                     /locus_tag="VN968_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL28076.1"
                     /translation="MTNWDLIRIFLEVARIGSFRAAAEQLNMSANFLSKRISALESAY
                     KTTLMTRHVDGIRLTPEGVQVLEAAKLMEDASFGLDRALSQTAPALSGEVRLAVTEGL
                     GTFWLAPRLVEFQRAYPGLLVDLKCEMRSADVLRLEADVAVQLQEPRNPDLKRIKIGR
                     LHVVPFVSPSYVEIYGMPNDMDDLRQNHRVVIQDAEQTRGKEMYQKYGRREQLGFVAM
                     RNNVSTAHLWSIAKGAGVGWLPTYVPALGDPLIPLDLGVKFELDIWLAYHPDAKKIPR
                     VRQLIEWTIQSFDGRKYPWFRDEFVHPNDLHKSYKEAEPLVNLFAGFKKISRPKVVRI
                     FPDRTNGTNR"
     gene            6894..8111
                     /locus_tag="VN968_02125"
     CDS             6894..8111
                     /locus_tag="VN968_02125"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL28077.1"
                     /translation="MNAFDIDLIRQSAMESAPASRSTAHSLDRMTALQLAKRIVIFTV
                     DAAGIAALVDKARQDIPGLTSNQMFQAVANHNPDTFWAIAKKDHFDITAPRGEGFFAV
                     LPLTHEGMRGLIDGSLDTRKPDISFIARQSERPAGIYIWAIHAKGSLVAAISLVLQKI
                     SAPLYRGVNLYSRPVTKEGLRVLEPLGFSPGARYDGLFAPNLQMFDRSNDVAAVKAAP
                     RDPVTVEVVRSMDEMARVIAIRGAIYMGEQRCPFEEEFDGNDFSATHLICHKDREPVG
                     CIRIRYFADFAKLERLAVRHEGRGSGLAGRILDAAIDLCRKKGYSVLYAHSQKRFLKV
                     WQQRGFKLMGARELVFSDFDYVEVKLETQKHPQSITLSDDPYVIIRPEGRWDTPGILE
                     RSASRGVCRSAGA"
     gene            8171..8704
                     /locus_tag="VN968_02130"
     CDS             8171..8704
                     /locus_tag="VN968_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HXL28078.1"
                     /translation="MVDLQEKNYDELAHDRASDLTRSLENCMSAIRHARNLGLPIAFT
                     RQTDSSESIERSAQSAWISGFEPKRSDMVFERPQPSCYTNRLFDDIVSQIGSFAIAGL
                     VAEETCLATAIDASHRGHHVTFLSDASASRGRHDADARAVHVVTTKAIELFADTVTTR
                     DWLVATTPRTPKGHRHG"
     gene            8697..8912
                     /locus_tag="VN968_02135"
     CDS             8697..8912
                     /locus_tag="VN968_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28079.1"
                     /translation="MDEVSARSLRNVISVLLQQRNIVVSRGASFAGHLIDLAIMQLRL
                     TVHEISEEELSEFSKLVRARCEHEHSD"
     gene            8930..10129
                     /locus_tag="VN968_02140"
     CDS             8930..10129
                     /locus_tag="VN968_02140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL28080.1"
                     /translation="MPGQRWMILAVLFAARAATGFQFQSVGSTATMLMRDLATDYSGI
                     GMLLGAYLLPGVIVAFPTGVLGQRFRDKTLGLVGLLLMAASAAVLGAAEGFAIALVAR
                     IIGGVGATIVALVATKMTTDRFDGREIVLAMSILQVSWPFGAMLALPIQAYIAQLWGW
                     PAVMISGALCAGAALGAFALVSQPSEQKQRTVTGRSRLPGGMLVQVLIAGTAWGAMNL
                     ACVLFFSYAPLLLVAQGSSSTAAASLTSLAVWFTIVSIPSGGYLIHRSGRPIAAIVVC
                     SLVAAAALALFVAEIHPTISCMVFGVFVGPLSGAILSLPSKVLAPRDRALGFGVFYTC
                     FYALTAVGPLAAGYLQDVWGSPSAALIAGAALLVAASPLCLAFEFLSNPRRLARTDRD
                     VELRAAS"
     gene            complement(10159..10632)
                     /gene="ybaK"
                     /locus_tag="VN968_02145"
     CDS             complement(10159..10632)
                     /gene="ybaK"
                     /locus_tag="VN968_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HXL28081.1"
                     /translation="MSKTTRATLALARLGVKFALHTYDYDSSADRIGLQAAEALGVEP
                     RRMLKTLMAEVDGKPVCVVVPSDREVSMKKLAAAFAGKAANMMRPAAAERLTGYHVGG
                     ISPFAQKKRVPVAIDPAALAETSVYLNGGQRGLQIELDPNDAVRALDATVQPLTG"
     gene            complement(10674..11003)
                     /locus_tag="VN968_02150"
     CDS             complement(10674..11003)
                     /locus_tag="VN968_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28082.1"
                     /translation="MFRWLVGIVILAVVISSDALSQQSLQELENMSQFKQALAAVDAI
                     KHRKNLQCVIAIANRALCGCLSWKLPVNIYFRSYASIASQAKGPEYGQLSAADKTIVD
                     QCVSDSR"
     gene            11451..12743
                     /locus_tag="VN968_02155"
     CDS             11451..12743
                     /locus_tag="VN968_02155"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HXL28083.1"
                     /translation="MANVVVVGAQWGDEGKGKIVDWLSEQADIVVRFQGGHNAGHTLV
                     INGETYKLALLPSGVLRPSKLAVIGNGVVVDPQAFLDEIAKLRGQGVAISPENLRVAE
                     NVTLILPLHRELDALRESSNAITAIGTTRRGIGPAYEDKVGRRAIRLMDLADLPTLPH
                     KIDRLLSHHNALRRGLNLEEIGAAGILQELTALAPKLLPYAETVWRLLDQKRREGKRI
                     LFEGAQGALLDVDHGTYPYVTSSNTVAAQAATGTGMGPGAVGYVLGICKAYTTRVGQG
                     PFPTELHDEIGEEIGRRGKEFGVNTGRKRRVGWFDAVLVRQTVRTCGINGLALTKLDI
                     LDGFDTIEVCTGYTLDGGEIDHLPAGEGAQARIKPVYETIEGWKKPTANARSWADLPA
                     QAIKYVRRVEELVGCPIALLSTSPEREDTILVQNPFEA"
     gene            12781..14511
                     /locus_tag="VN968_02160"
     CDS             12781..14511
                     /locus_tag="VN968_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28084.1"
                     /translation="MADYYPLIARAISGLDPSAPGESRRALYERARAALIAQLRSVQP
                     PLSESEITRERLSLEEAVRKVESEAAQRARDATRPGGDATRSADSTRAGGDRPRAGDS
                     FRANIRPTPRPGEPPSASLLQNSLSPPSASQPSTSPPSTSLPSPPPRPRPPSTLQPRN
                     ERPPLGLEQERPDARPPRNLRADAPPIPRPQPPQSQPQVSMQDPQLPPTRERAGAPRR
                     GPDNGASQAQAPGMRGFRDIAADADDLGRAAAQASRSARKTYANVPSPSPEFDRLEPS
                     MENRGADPDAPYSYDESAEEAERYDAPPPLPSRPRPTPERGPKKAGRAGLFPFKSAIA
                     VGVVLILVGAGILWGKSVVTGVSGLFKSSTTVVEAPKDASTPLSKPKIADRVGQPSPT
                     DQVAPVAQRVVLYDEDPSDPKGKQYVGTVIWRTEQIKAANSPTADIAVRADIEIPDRK
                     FKMTMSFRRNTDTSLPASHTAELTFILPPDFVGGGVGNVPGILMKSNEQARGTPLAGL
                     AVKVTDGFFLVGLSNVDSDRARNLQLLKERSWFDVPLVYVNQRRAIIAIEKGAPGERA
                     FNDAFATWGE"
     gene            14740..15156
                     /locus_tag="VN968_02165"
     CDS             14740..15156
                     /locus_tag="VN968_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5413 family protein"
                     /protein_id="HXL28085.1"
                     /translation="MKRYLIFAALGPLLGGFWFLLTASVMSSYWSHPPSVSEAAKLFA
                     VFARTVQYSYLFGILPALMLGALDDILFHVKRIGWRVRLLIVGAVAFIMAELLYGSRG
                     PDSGAVQFIMYGLVGFIPGTISSWLAHETVEQPVAA"
     gene            complement(15501..15755)
                     /locus_tag="VN968_02170"
     CDS             complement(15501..15755)
                     /locus_tag="VN968_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28086.1"
                     /translation="MNRLIAATFLVSTLMGAGFAHAMPLAPLVPASSADVIEVAGGCG
                     PGWHRGPYGGCLRNFANPAAHACPRGFHIGPNGGCRGNGR"
     gene            complement(15799..16038)
                     /locus_tag="VN968_02175"
     CDS             complement(15799..16038)
                     /locus_tag="VN968_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28087.1"
                     /translation="MTTSSVRAMLLGAAITASVGVGYAIGAQPHMNESIALLLSARGE
                     LQAATPNKGGHRERAMGLIDQAISEVRAGIAFAGG"
     gene            16433..17395
                     /locus_tag="VN968_02180"
     CDS             16433..17395
                     /locus_tag="VN968_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL28088.1"
                     /translation="MPVIKKMLSTDMLPRGLDDRQRFLSFAELFEHFSNTGELDPASD
                     VPFRAAMNSIHIGTTMLGRCDGTFTTVRRERRQVLETNDDRYCLARNTGDRESLVIHR
                     GRAFTLRPGAMVLLKLDEPFFCADGASHKRFTNVHLPMATLKAMVAGVDDLVGRELAG
                     GGALSLAMDYSDLLLRHTGAVDEAGMAIAAHLTDLVALGLGARGDLALAARRGGLRAI
                     RLKAVLMILEKRFTEPDFSAQKLATATGLSERYVNELLYEAGASFTMRLNELRLRKAA
                     ELLTRGAEGRISDIAFDCGFNDLSYFNRCFRRRFGLTPSAARGR"
     gene            17677..18189
                     /locus_tag="VN968_02185"
     CDS             17677..18189
                     /locus_tag="VN968_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HXL28089.1"
                     /translation="MYSRTTARSQGPFRGSKTHGPIIDQEGREIPAEALGPQVRGFQF
                     DFGNSTANPFANLTREQRLARLDAVAKLLDIAFIVPGTNIRYGIDGIIGLIPVVGDII
                     ATALSLWLVREARALGAPWHVTARMLGNVAVQGVIGTVPVAGDAFDVLFRANMRNARL
                     LRRWMDRQPR"
     gene            complement(18423..18719)
                     /locus_tag="VN968_02190"
     CDS             complement(18423..18719)
                     /locus_tag="VN968_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28090.1"
                     /translation="MASFSYNTAAELFPAAIRKKKRAGFAYRRFDTAAAAVQFAIEEL
                     PADSLNGAYLQVDEARFDQSGIRTLYESQDFPLPRRPRAPEPAPASNDADADAA"
     gene            complement(18930..19094)
                     /locus_tag="VN968_02195"
     CDS             complement(18930..19094)
                     /locus_tag="VN968_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HXL28091.1"
                     /translation="MSLGLILVIILIIFLLGGFSGRFGGYGYGYGHSGMGLFGVILVV
                     LLILLLLGKL"
     gene            complement(19197..20084)
                     /locus_tag="VN968_02200"
     CDS             complement(19197..20084)
                     /locus_tag="VN968_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL28092.1"
                     /translation="MQDFTSAHIETGETSIFVRISGSGTPLLLLHGFPQTHVMWRGVA
                     PLLAHEFTVVCADLRGYGQSGCPASAPDHAPYAKRAMARDMVTVMERLGFTRFSVAGH
                     DRGGRVAYRMALDHPDRIERLAVLDILPIETAWERADADFTLGYWPWSLLAQPEPLPE
                     TILATSAEAIIDNALGGWGSPSQAFPLEVRAAYIEALRDIGHAHAICEEYRAAASIDR
                     AHDRADRDAERKIRAPVLAIWSAQGPLDSWYAEGPVTLWRQWAGKVRGYALKGGHFFP
                     EAAPELTAQALRRFFAHSD"
BASE COUNT         3790 a         6399 c         6424 g         3866 t
ORIGIN      
        1 gcaaagtggg catccgtgat gcgtgcgccg ctgatcgcca tcgttctctg cagtgttggc
       61 ggtgccgttt gcgctcagcc tgcggacgag gcctccaaaa tgttagacac gtgctttcag
      121 ttggcccgca ccgcagacgc gatgtgttcc aacccgaaaa atggtgaggc tgagcgcctg
      181 gactgccgtc aaaaggcaag catggcacag ctcgaatgcc tggagcgcgt tttcccggga
      241 cggactgctg aatccgctcc gccagaaatg cccgctcgca ccgtctcgcc ggagatgccc
      301 actgttaccg cttcgccgga agtgcctgct gggtccgttt caccggagag gccgattgga
      361 accctggcca aaggtgagcc cgccagctcg caaaaaacgc gtgccagaac agctccggca
      421 gaaacgcccg ttgcaaccgt ctcgccggag atcacgactg gcaccgcatc gcccggcgta
      481 cccgcgaggc acgtcgatat ccctgcaatg ccacaggata cacattggat cgtaagtgaa
      541 acgacctcgc cggtggatta cagccctctc gtcacggcgg tgatccgctc gaccaccagc
      601 gtgcaggatg caccgaacac gctggtcatt cgatgtcttg gaccgcgcac cgaagtcctg
      661 cttcgcacgc aaggaacatg gcgcgcatct cgttccaaca cagttcaagt cgactatcag
      721 atcgacgacc aacccctcgc cagactacaa tggattacgt ccgcagatgg aaagacagcc
      781 agttacggca ccgatgccgt cgggtttctg cagtcgctgc cggaagacgc gcgactgaaa
      841 atcagcgtgc tcgatcgagc gggccccggt cacgaagcga cgttccagct cgccgggttg
      901 gacgccgtcc ggaaaagaat tgccgtgcca tgcaaatggg cgacggcggc agacaagatc
      961 tcatcgggaa aacgatagac ccggatggtc gaacccagct ttttttggcg tcacacgtca
     1021 cattaagtaa gtttgttacg gccggacgta actccagccc ttctcctgca gttccatcaa
     1081 ccgaacgacg cccgacggaa cgatgacggc ctcagacagc acagtgatcg ggtggccctc
     1141 ttttttctcc atgccttcct tggtgttgcc gcatgcagag aactggacct tgctgggaaa
     1201 tgcatgatcc ttcaaactct tgatgcgatc ctgtaccggt gatgtgtcgg cacgcagcat
     1261 gtgcaagccg gggccatagg tgatgacatc cacatctacg tcctctccct tggcgcgata
     1321 ataatcaatg acgttcgtgg cgttattgag cgcgaggttc atgaccgccg gatcattctg
     1381 atcgacctga atgaccaggc ggtgcgcttt cgtggcatgt tgcgctttcg ttttagctgc
     1441 tgaacctgcc gagtgatgcg cgctttttgc agggtgcgct ttggtagaac gctgcacttt
     1501 cgtatcatct gccaggcttg ccgaaataag ggacagcgac gtaaaagcga cgatcgtcac
     1561 ctgacgaaac aatttacgca ttatgatgct ccttcgagcg aaccagagta agattcctgc
     1621 catctgaaaa tttgctcgcg ccactgctcc tgttgagttc atggttgcct ccttggtaag
     1681 cgacctttga cagtcatcta aatcccgcga tttcgaggaa gcaacgtcca aatcatgccc
     1741 ggccggcact ttctcacggc tcctgatcct cgctgcgatg cggcaacggt ccctcgcgct
     1801 ccaaaacagc gccgagatcg cgcatgtcga atgtcccgat ccaggcggcg tctcgatttc
     1861 cgcagagggc cccgaagtgg tcggttcgca agggatgtcc gctttggccc ggcgaacagt
     1921 cgtcgcgcgg aacgaaggcc aggtccgaaa agtgccaatt tcgcttcgtt ggctaatgcg
     1981 ctttcgccgg cttgcgtgac gcgacgaaca cgccggtcag caccagcgca aagccgatga
     2041 tgtggaacag ctgcgggcgc tcgccgagaa agacgatcgc catcgccgat ccgaacaccg
     2101 gcacgacatg aaagaatggc gcggcgcggt tggcgccgat cagctgcacg ccacgattga
     2161 agcacagata ggcgagcgtc gacggaaaca ccgcgacgta gaacagcgac agcaggttcg
     2221 ccgcatcgag ccgcatcacc gggcgcgcga acagttccca gatccaaagc gggaccaggc
     2281 acacggcgcc gcagccgaag gtgaaggcga caaacgacag gccgtgaatc gccggacgct
     2341 tcagcgacag caccgagtac agcccgaaga tcgccagcgc catggtgaag atgatatcgc
     2401 ccttgttgaa cgcgatgctg gtgagcgccg tcaggtcgcc gtgcagcagg atcaccagca
     2461 cgccggtcag cgagaccgcg atcccggcgg cctgcgccag cgtcaggcgg atgccgagca
     2521 ggatcagcga ccagaccgcg acgaacagcg gcccggccga ctgcagcagc agcgtgttca
     2581 gcgccgtggt gtgttcgagc gaccagtatt gcagggtgtt gaaggcgctg atcccgatga
     2641 cggagagaaa gatcatggtg ccgagatggc tgcggatcgc acccaggtcg cgcttcagat
     2701 gcgtccaggc gaaggggaga atgacaagga acgccagcga ccagcgcagg aacgacagcg
     2761 tcaccggcgg aatatgcccg gcggcgaggc ggccgacgat ggcgttgccg gcccagcaca
     2821 gcgcggtgat cgagagcagc agatagggct ggttggcgag ccagttgccg aagggggcgg
     2881 cgctggaggt gtgatcggag gctgacatgc ggcggtcgtt cttggcccgg ttccatgacg
     2941 ccgcggcccg gcccggtgta cggcaccggg ccggaaatca tggcccggcc gcctcacaga
     3001 cacggattct caccgcgcat caatggcatg gatgcattgc gaccatgcac cagcaggcat
     3061 tttgtggctc gggtccgatt gtggctcggg tccgatcaac accggttgga aatcgccggc
     3121 gccatcgtct atcctgcggc gatgttgcaa aagttcattc tcgctctggc gatcggcgcg
     3181 ggctgcgccg gggttgcgat ggccgagccg cgctccggca tcgatatttc ggcgatcgat
     3241 ccgagcgtgc ggccgcagaa cgatttctgg caattcgcca acggcaaatg gctcgcggcg
     3301 acgtcgattc cagccgaccg cgccgcctgg gataccttct ccgcgctgcg cgaaaccaac
     3361 cagcagcagt tgcgtgaggt gatcgaagcg atcgatccgc gcagcccgga cggcggcgag
     3421 ccgcgcaagc tcgccgattt gtatggctcg ttcatggacg aggcgcgggt ggaagcggcg
     3481 ggcctggacg gcttgcgcga cgaactgcgg cggatccacg cattgagcga caaggcggct
     3541 ctgcccgaat tgttcgcgca tctttcccga ctttcggtgc gcatcccctg ggggctggag
     3601 atcggaccgg atgagcgcga tgcgacgcgc tatgtcgcgc atctggagca gagccgtttg
     3661 ggattgccgg atcgcgatta ttatctcaag gacgacgcgc atttccaggc gatccggacc
     3721 ggctatcgcg accacatcgt caaattgctg gcgctcgcgg gcgaaccggc ggcggagacc
     3781 agcgccggcg agatcatcgc gctcgagaac aggctggcgc gcctgcagtg gacgcgggtc
     3841 gagaaccgcg atccgctcaa gacctacaac aagcgggata tcgcggcact gccggcgctg
     3901 accacaaaag acgactggcc gtcctttctt tccgccggcg gtgtcggaac ggaaattcgc
     3961 agcattgtgg tggcgcagcc gagctatttc gacggcgtcg gcgaaattgt tcgcgaggtg
     4021 ccgctggcaa cctggcaggc ttacctcgcc tacaacctgc tgagcaccta tgcgccctat
     4081 ctttcggcgc cgttcgtcgc cgaggaattt gccttcgagc agcacacgct gcgcggcgtg
     4141 ccggaaatcc agccgcgctg gaaacgcgcc gttgccttgg tcgatcgcct gaccggcttt
     4201 gcgctcggcc gactttatgt tgaacgctat tttccgccgg ccaacaaggc gcgggccgac
     4261 gccatgatcg caaatctgac ggcggcctat cgcgagagca ttacgacact cgactggatg
     4321 gggcccgaga cacgccgcga ggcgctcgcc aagctcaagg ccatcgttcc gaaaatcgcg
     4381 tacccgacga catggcgcga ctacagcggg cttgtcatcc ggcgcgacga cctggtcggc
     4441 aatgtcatgc gcgcgcgccg cttcaactac gatttctggc tggccaagct cggccacgaa
     4501 ttcgaccgcg cggaatggct caccacgccg cagacggtca acgcatatta cagtgccagc
     4561 cgcaacgaga ttgcgtttcc cgccggcatc ctgcagccgc cgttcttcga cgccgaggca
     4621 gacgacgccg ccaattacgg cgccatcggt tccgtgatcg gccacgagat cagtcacgcc
     4681 ttcgacgacc atggcagccg gttcgatggc gacggcaatc tgcgcaactg gtggaccgaa
     4741 gacgatcgga ctcgcttcga aggcaaaacc cgcagcctgg tggcgcagta cgacgccttc
     4801 agcccgctgc ccggtcacca cgtcaatggc gcgctgacgc tgggggagaa cgttgccgac
     4861 aatgccggcc ttgcgatcgc cgagcgggcc tatcgccttg ctctcggcgg ctgccccgcg
     4921 ccggacctcg acggctatac cggcgagcaa cgcctgttca tcagcttcgc gcaaatctgg
     4981 cgcgacaagg tgcgcgatgc cgcgcgtatc gaacgtctca aggtcgaccc gcattcgccc
     5041 ggacagttcc gcgccgacgg cacgctgcgc aaccagagtg ccttcgccga agcctttgcg
     5101 gtcaagccgg gcgacgcgat gtatctgccg ccggagcagc gcatatcgct gtggcagacg
     5161 gacagtgaag tggtgtgcaa ggaaaaggcc cgttagcgca tgatccggaa aagtggaaac
     5221 cggttttccg aaaagatcat gctcaaacaa agagatgaga tcatgatccg attcaatcta
     5281 atcggatcat gatctagcgc gcgtcgggtg acgcccttcg acctctggaa ttggctagcc
     5341 gccgtctccg ccccggctgg cctgacattt gacgatctgt tcggccagaa gaatcagcac
     5401 ctccttggtt cgcagatccg tcatatcctg aaaagcccgc agcaggcgca acgcgagcac
     5461 cgattgcagc gaattgatgc gctgcgccga cgaaacatct ggtgcttcac gttcggtacc
     5521 aatggtctgg tcgtagaaga agtcgatcgg tatctgcaat gcttcggcga cccgcatcag
     5581 gcggtccgcg tcgacgtaat tcgatccgtt gccgtcttcc gatgtcagct gaagcgtgcc
     5641 accgagaact tcggccagat cggcttgcga aagcccgatc gcgagccatc gtgccttcac
     5701 gacctggtca acgtgaccgt ccgtggcgcc gcccgtcatc ggttggttcc attggtacga
     5761 tcgggaaaaa ttcgcacgac tttcgggcgc gatattttct tgaagccggc aaataaattc
     5821 accagcggct ctgcttcctt gtacgatttg tgcaaatcgt tcggatggac gaactcgtcg
     5881 cgaaaccatg gatatttgcg gccatcgaac gactggatgg tccactcgat caattgcctg
     5941 acgcggggta ttttcttcgc atccggatga taggcaagcc agatatcgag ttcgaattta
     6001 acgccgagat cgagcggtat cagcggatcg ccaagcgccg gcacataggt cggcaaccag
     6061 cctacgccgg cgcctttcgc gatcgaccaa agatgggcgg tagagacatt gttgcgcatc
     6121 gcgacgaagc cgagctgctc gcggcgtcca tacttttggt acatttcctt gcctctggtc
     6181 tgctccgcat cctgaatgac gactcgatga ttttgacgga gatcgtccat gtcattcggc
     6241 atgccgtaaa tttcgacata ggacgggctg acaaagggca cgacatgaag ccgtccgatt
     6301 ttgattctct tcaggtcggg attccggggc tcctggagtt gcacggcgac atcggcttcg
     6361 agacgaagca cgtcggcgga acgcatttcg catttcaaat ccaccagcag gccgggatag
     6421 gcccgctgaa attcgaccag cctcggcgcc agccaaaacg tgcccagccc ctcggtaacg
     6481 gcgagccgaa cctcaccgct cagcgccggc gcggtctggc tcagcgcgcg gtccagtccg
     6541 aaggacgcgt cttccatcag ctttgccgct tcgagaactt gcacgccttc cggcgtcaat
     6601 ctgatcccat cgacatggcg cgtcatcaat gtcgttttgt aggcgctttc cagcgctgaa
     6661 attcgcttgc tgagaaaatt cgcagacata ttcagttgtt cggccgcggc ccggaagctg
     6721 ccaattcggg ccacttcgag aaatatccgg atcagatccc aattcgtcat tcgtccgggt
     6781 gtgcgcgcgc cagcgtgcgc acccctgcgc tcacgtgagt gcatacaaaa gcctcccgtt
     6841 tcaccattct attaatggca gaaacatccg gtctatacaa tcgagagacg atcatgaacg
     6901 cattcgatat cgatttgatc cgccagtcgg caatggaaag tgctccagcg agccgatcca
     6961 cggcccactc actcgacagg atgaccgctc tgcagctggc gaagcggatc gtcattttca
     7021 ccgtcgacgc ggcgggtatc gcggccttgg tggacaaggc ccggcaggac atccccggcc
     7081 tgacttcgaa ccaaatgttc caggcggtcg ccaatcataa tccggacacg ttctgggcga
     7141 tcgcgaagaa agatcatttc gatatcaccg ctcccagggg cgagggattt ttcgcggtgt
     7201 tgccgctgac gcacgaaggc atgcgcgggc tcatcgacgg cagtcttgat acgcgcaagc
     7261 ccgatatttc gttcatcgcc cggcaatcgg agcggcccgc gggaatctat atctgggcga
     7321 tccatgccaa gggcagcctc gtcgcggcga tatcgcttgt cctgcagaaa atctcggcgc
     7381 cgctttatcg aggcgtcaac ctctattcac ggccggtaac caaagagggg ctgcgggtgc
     7441 tcgagccgct cgggttttct ccgggagcgc gctacgacgg attgttcgcg cccaacctgc
     7501 agatgtttga tcgctcgaac gatgttgcgg cagtcaaagc cgctccccgg gatccggtaa
     7561 ccgtcgaggt cgttcggtcg atggacgaaa tggcgcgcgt catcgccatc cgcggcgcga
     7621 tctacatggg cgagcagcga tgcccgttcg aagaggaatt cgacggcaac gatttttccg
     7681 ccacgcatct gatctgccac aaggaccgcg agccggtcgg atgcatccgg attcggtatt
     7741 tcgccgattt tgccaagctt gaacgactgg cggtccgcca tgagggccgg ggttcgggcc
     7801 tggcggggcg gatcctcgac gcggcgatcg atctctgccg caagaaaggc tacagcgttc
     7861 tgtacgccca ctcacaaaag cgttttctca aggtctggca gcagcgcggt ttcaagctca
     7921 tgggcgcccg cgagctggtg ttctcggatt tcgactatgt cgaggtcaag ctggaaaccc
     7981 agaagcatcc gcagagcatc acgctcagcg atgatcccta cgtgatcatc cgccccgagg
     8041 gccgctggga tacgcccggc attcttgaaa ggtcggcgag caggggtgtt tgccggtcgg
     8101 cgggcgcttg atgaactcag tcatagatct gcggacctac gtcagcgcgt cgcatagccg
     8161 tctgcttgtc atggtcgacc tacaggagaa aaactatgac gaattggcgc atgaccgggc
     8221 atcggacctt acgcggtcat tggagaattg catgtccgcg atacggcacg cgcgaaatct
     8281 ggggctgcct atcgcgttca cgcgacagac cgatagttcg gaatcgatcg aaagatcggc
     8341 ccagtccgca tggatttcag gtttcgagcc gaaacgttcc gatatggtgt tcgaacgccc
     8401 gcaaccctcc tgctatacca atcggctctt tgacgacatc gtttcgcaga tcggaagctt
     8461 cgcaatcgcc ggcctggtcg ccgaagagac atgtctcgca accgcgatcg atgcgtcgca
     8521 tcgaggccat cacgttacat ttctgagcga cgcgtccgcg agccgcggcc gccatgacgc
     8581 ggatgccagg gcggtccacg ttgtaaccac gaaagccatc gaattgtttg ccgacaccgt
     8641 caccacgcgt gactggctgg tcgcgactac cccgagaacc cccaagggac accgacatgg
     8701 atgaagtcag tgcccgtagt ctacgtaatg tcatttcggt gttgctgcag caacgcaaca
     8761 tagtcgtttc caggggcgcc tcttttgccg gtcacttgat cgatttggcg atcatgcaac
     8821 ttcgcttgac ggtgcacgag atatccgagg aagagctttc cgagttttcc aaattggtcc
     8881 gcgcgcgctg cgaacacgaa cactcggact gatcggggga ggcgagccga tgcccggcca
     8941 aagatggatg atccttgcgg tgctgttcgc ggcgcgcgcg gcaaccggat tccagtttca
     9001 atcggtcggc tcgaccgcga cgatgctgat gcgggatctg gcaaccgatt actccgggat
     9061 cggcatgctg ttgggcgctt atctgttgcc cggcgtcatt gtcgcgtttc cgacgggagt
     9121 gctcggccag cggtttcggg acaagacgct cggcctcgtc ggcctgttgc tgatggcggc
     9181 ttctgcggcg gtgctgggcg ctgccgaggg tttcgcgatc gcgcttgttg cgcgcatcat
     9241 cggcggtgtt ggtgccacga tcgtggctct ggtcgccacc aagatgacga ccgaccggtt
     9301 cgacggccgc gaaatcgtgc tggccatgtc gatcctccag gtaagctggc cgttcggtgc
     9361 gatgctggcg ctgccgatcc aggcctatat cgcgcagctg tgggggtggc ctgccgtgat
     9421 gatatccggc gcgctgtgcg ccggcgccgc gcttggcgcg ttcgcccttg tttcgcagcc
     9481 atcggagcag aagcaacgga cggtgaccgg tcgctcgagg ctccccggcg ggatgctggt
     9541 tcaggtcctg atcgccggca cggcgtgggg cgcgatgaac ctcgcctgtg tcttgttctt
     9601 cagctacgcg ccgttgttgc tggtggcgca gggatcatcg tccaccgccg ccgcctcgct
     9661 gacgagtctc gccgtctggt tcacgatcgt atccattcca tcgggtggat acctcattca
     9721 ccgctcgggc aggccgatcg cagcgatcgt cgtctgctcg ctggtcgctg ccgcggcgct
     9781 tgccctgttc gtcgcggaga tccatccgac catctcctgc atggtattcg gagtgtttgt
     9841 cggtccgttg tcgggagcga tcctttcgct tccatcgaaa gtgctcgccc cgcgtgatcg
     9901 cgcgctcggc ttcggcgtgt tctatacctg cttctatgcc ctgacggcgg tcgggccgtt
     9961 ggctgccggc tacctgcagg acgtctgggg atcgccgtcg gccgcactga tcgcgggcgc
    10021 cgcgttgctt gtcgcagcct cacccttgtg ccttgcgttc gaattcctgt cgaatccgcg
    10081 tcgtctcgcc cgtaccgacc gtgatgtgga actgcgcgcc gcttcgtgac cgatgcactc
    10141 aggggtttgc ggcggtgctc accccgtcag cggctgcacg gtcgcatcga gcgccctgac
    10201 ggcgtcgttg ggatcgagtt cgatctgcaa cccgcgctgc ccgccattca gatacacgct
    10261 ggtctcggcc agcgccgcag ggtcgatcgc gaccggcacg cgctttttct gtgcgaacgg
    10321 gctgataccg ccgacatgat atccggtcag ccgttcggca gcggccggcc gcatcatgtt
    10381 cgcggccttg ccggcaaacg ccgccgcgag tttcttcatg ctgacttcgc gatccgacgg
    10441 cactacgacg cagaccggct tgccatcgac ctccgccatc agcgtcttca gcatgcgtcg
    10501 cggttcgacg ccaagtgcct cggcggcctg caggccgatg cggtcggcgc tggaatcgta
    10561 gtcgtaagtg tgcagcgcga atttgacgcc cagcctggcg agcgccaatg tcgcgcgggt
    10621 ggttttcgac attagcgtta tcgctccgtc atcgcgttcg ccggagttcg cttctatcgg
    10681 ctgtcgctga cgcattggtc cacaatcgtc ttgtccgcgg ccgacaattg tccgtactcc
    10741 ggcccctttg cctgactggc gatggaggcg tagctgcgaa aatagatgtt cacgggtagc
    10801 ttccaggaaa gacagccaca aagtgccctg tttgcgatgg ctatcacgca ttgcaggttt
    10861 ttccgatgct ttatcgcatc gacggcggcc agtgcttgtt tgaactggct catgttttcc
    10921 aattcctgga gtgactgctg ggataacgca tcggaagaga tgacgacagc aagaataaca
    10981 atgccaacca accatcgaaa catccttggc ctcccgttcg gtgccttgca cctggtatat
    11041 ttagcagcct cgctgcaacg aaagagacag ccgattggag cactggccgc gcggttgctt
    11101 gcggactgac ggtgtcgaag gggatgatgt caccgaccgc acgatcggtg attgattgac
    11161 ggccgcttcc gggcgtaatg gcggctgccg ggaacccaaa gtagcatgtt gaaaagctgt
    11221 ggacaccgat ccggcccggg ttctggccca atgctgccgc accccggccg cgaggcttga
    11281 attttcaggg gatatcaatg ggttgggagt gtcccaagcg gggtccgcaa acccgttctt
    11341 gcttgcgtcg atagcccgca tcctttatcc ggtggggagc ctcgcgtgcg agcggccccg
    11401 gccgtgtttt tggctgggcg tcgagataat tgcagaggga acagctgcga atggccaacg
    11461 tcgtcgtcgt cggcgcccaa tggggtgacg aagggaaggg caagattgtc gattggttgt
    11521 cggagcaagc cgatatcgtc gtgcgcttcc agggcggcca caatgccggt cacactttgg
    11581 tcatcaatgg cgagacctac aagctggcgc tgctgccctc cggcgtgctg cggccttcga
    11641 agctcgcggt catcggcaat ggcgtggtgg tcgatccgca ggcgttcctc gacgaaatag
    11701 cgaaattgcg ggggcagggc gtcgcgatca gcccggaaaa tctgcgggtc gccgaaaacg
    11761 tcacgctgat cctgcccttg catcgcgaac tcgacgcctt gcgggaatcg tccaatgcga
    11821 tcaccgcgat cggcaccacg cggcgcggca tcggccctgc ctacgaggac aaggtcggcc
    11881 gccgcgcgat ccggctgatg gatctcgccg atcttcccac gctgccgcac aagatcgatc
    11941 gtctgctgtc gcaccacaac gcgctgcgcc gcgggcttaa tctggaagaa atcggcgctg
    12001 cgggaattct gcaggagctg accgcgctgg cgccaaaact tctgccttac gcggaaacgg
    12061 tgtggcggct gctcgatcaa aaacgccgcg aaggcaagcg cattctgttc gagggcgcgc
    12121 agggcgcgct gctcgatgtc gatcacggca cctaccctta cgtcacctcg tccaacaccg
    12181 tggcggcgca ggcggcgacc ggtaccggca tgggtccggg agcggtcggc tatgtcctgg
    12241 gcatctgcaa ggcctatacg acaagggtcg gccaggggcc gtttccgacc gaattgcatg
    12301 acgagatcgg cgaggagatc ggccgccgcg gcaaggaatt cggcgtcaac accgggcgca
    12361 agcggcgcgt cggctggttc gacgcggtgc tggtgcgcca gacggtgcgg acctgcggca
    12421 tcaacgggct ggcgctgacc aagctcgaca ttctcgatgg tttcgacacg atcgaggtct
    12481 gtaccggcta tacgctggac ggcggggaaa tcgaccatct gccggcaggc gagggcgcgc
    12541 aggcccgaat caagccagta tatgagacta tcgaaggctg gaagaagccg accgccaatg
    12601 cccgctcgtg ggcggatcta ccggcgcagg ccatcaaata tgtccgtcgc gtggaggaac
    12661 tggtgggctg cccaatcgcg ctgctttcca ccagccccga acgcgaggat actatcctgg
    12721 ttcagaaccc gttcgaggct taacgcgatt ccaccggaaa gcccgcaatg ttgaaaagat
    12781 atggctgatt actatccgct gatcgcccgc gctatctcgg gactggaccc cagtgctccc
    12841 ggtgagagcc gccgcgcgct gtatgagcgc gcgcgcgccg cgctgatcgc gcagcttcgc
    12901 agcgtgcagc cgcctctgag cgaatccgaa atcacccgcg agcggctgtc gctggaagaa
    12961 gccgtgcgca aggtcgagtc cgaggccgcc cagcgcgccc gggacgcgac gcgccccggc
    13021 ggggacgcga cgcgttcggc ggacagcacc cgcgccggcg gcgaccgccc ccgcgccggc
    13081 gattcgttcc gcgccaacat ccgccccacg ccgcggccgg gcgagccgcc gtcggcttcg
    13141 ctattgcaga attcgctatc gccgccttcg gcatcgcagc cttcgacatc gcccccctcg
    13201 acgtcgctgc cttcgccgcc gccgcgcccg cgtccgccat cgacgctgca gccgcgcaat
    13261 gagcgtccgc cgctcggact ggaacaggaa cggccagacg ctcgcccgcc gcgcaacctt
    13321 cgcgccgacg cgccgccaat accgcggccg caaccgccgc agtcccagcc gcaggtttcg
    13381 atgcaggacc cgcagctgcc gccgacccgc gaacgcgccg gcgcgccgcg ccgcggcccc
    13441 gacaacggcg cctcgcaggc gcaggcgccg gggatgcgcg gttttcgcga tatcgccgcc
    13501 gacgccgacg atctcggccg cgccgcggcg caagccagcc gctccgcgcg aaaaacctat
    13561 gccaatgtgc cgtcgccctc gccggaattc gaccggctcg agcccagcat ggaaaaccgc
    13621 ggcgccgatc ccgatgcgcc ctattcctat gacgaatccg ccgaggaggc cgagcgctac
    13681 gacgcgccgc cgccattgcc gtcgcgtccg cgcccgacgc cggagcgcgg gccgaagaag
    13741 gccggccgcg ccgggttgtt tccgttcaag agcgcgatcg cggtcggcgt cgtgctgatc
    13801 ctggtcggcg ccgggatcct gtggggcaag tcggtggtga ccggcgtgag cggcctgttc
    13861 aagtcctcga ccacggtggt ggaggcgcca aaggacgcgt cgacgccgct gtcgaagccg
    13921 aagattgccg atcgcgtcgg ccagccgtcg ccgaccgatc aggtcgcgcc ggtggcgcag
    13981 cgcgtggtgc tgtatgacga ggatccgtcg gatcccaagg gcaagcagta tgtcggcacg
    14041 gtgatctggc gcaccgaaca gatcaaggcc gccaacagcc cgaccgccga tatcgccgtg
    14101 cgcgccgaca tcgaaattcc cgaccgcaag ttcaagatga cgatgtcgtt ccgccgcaac
    14161 accgacacct ccttgccggc gagccacacc gcggagctga ccttcatcct gccgccggat
    14221 ttcgtcggcg gcggcgtcgg caacgtgccg ggcattctga tgaaatccaa cgaacaggcg
    14281 cgcggcacgc cgctggcggg gctcgcggtc aaggtcaccg acggcttctt cctggtcggc
    14341 ctgtcgaatg tcgattccga ccgcgcccgc aatttgcagc ttctgaagga gcgctcgtgg
    14401 ttcgacgtgc cgctggtcta cgtcaaccag cgccgcgcca tcatcgccat cgaaaaaggc
    14461 gcccccggcg aacgcgcgtt caacgacgcg ttcgcgacct ggggggagta ggggcgcggc
    14521 ggggtttgtc gttgtccatc ggtgcgagat ggacagctga taagtccgct gatgaccgcc
    14581 tgctcactgg gaatccatca catattttgc gatgccatcc cggttgagtt taggcaccga
    14641 cgatgcgtcg gaaaaggccc tgggaccagc gcagacagtt gacgcaattc ccacatgagc
    14701 gaaactgcct cccgggcaaa acgcgcgagg aggcgggcca tgaaacggta tctgattttc
    14761 gcggctctcg ggccgctgct cggcgggttc tggtttttgc tgaccgcctc cgtgatgtcc
    14821 agctattgga gccatccgcc cagcgtttcg gaggcggcaa agctgttcgc ggtgttcgcc
    14881 aggacggtac aatacagtta tctgttcggc atcctgccgg cgctgatgct cggcgcgctc
    14941 gatgatattc tgttccacgt caaacgaatt ggctggcgcg tccggctgct gatcgtcggc
    15001 gcggtcgcgt tcatcatggc cgagttgctc tatggctccc ggggaccgga cagcggggct
    15061 gtccagttca tcatgtatgg cctggtgggc tttatccccg ggacgatctc gtcgtggctg
    15121 gcgcatgaga ccgtggagca accggtggcg gcgtgagggt ttgctcgtca gggtctattc
    15181 cggaacaaac gagcggacgc aataactcgt ttgcgtcgtc ccggcgaagg ccgggaccca
    15241 taacccctgg cgctcttgtt gcgtcgtgcg gccgcgcaac cgctgccttt ctcatgatcg
    15301 gcacggcgac gcgatgagcg caattgcgct cacgcggggg gtcccggcct tcgccgggac
    15361 gacgatgtgc tgcagtagcg ttacgtgacc gaaaagcccg cagcctacgg ttctgattga
    15421 atcagaaccg aaggttctat ccttttgttt ggatgtgttt tcttcacgcg aaccgttagt
    15481 cgaactcgct cgaaagcacc ttaacggccg ttgccgcggc agccgccatt ggggccgatg
    15541 tgaaagccgc gcgggcaggc atgcgcggcg gggttggcga agttgcgcag gcatccgcca
    15601 tagggcccgc gatgccagcc ggggccgcat ccgccggcga cttcgatgac atcggcgctg
    15661 cttgccggca ccaggggcgc cagcggcatc gcgtgggcga aacctgcacc catcagtgtg
    15721 ctgacaagga aggtggctgc gatcagcctg ttcatgaact gtctcctcta aatggtttga
    15781 atgaaagggg tggccgcgtc agccgccggc gaacgcgatg ccggcgcgga cttccgatat
    15841 cgcctggtcg atcaggccca tggcgcgttc gcgatggccg cccttgttcg gcgttgccgc
    15901 ctgcagttcg ccgcgggccg atagcagtaa ggcgatgctt tcgttcatgt gcggctgcgc
    15961 gccgatcgca tagccgacac caacgctggc tgtgatagca gcgccgagca gcatggcgcg
    16021 gacggaagag gtcgtcatgt cgtttctcct ttgacttgta ttttgaaaaa acaggcaatc
    16081 ccggttgcga aagcgtgacg ccggatctga cgcgacatgg ctgattgctg cccgattcca
    16141 gcggccgttc ccgccgcatc ggtaagccag cgtttggcct tcgtcttgga ctggcgggcg
    16201 tccggggaat gcggatttgg actgtccggc gctgttttat tcacagccaa gtcctgctag
    16261 taaagtctcg gtagtttgtg ttgcccgttt tgtgttgccc cgttttgcaa aattcttcag
    16321 cccgcgttca ttccgcggcc ggcaggctgc cggcgtcgct tccagatcct tcccaccccc
    16381 aagtccttcc gctccccaag tccttccgct ccccaagtct ttccgctccc cgatgcccgt
    16441 catcaagaaa atgctgtcga ccgacatgct gccgcggggc ctggatgacc ggcagcgctt
    16501 tctttccttt gccgaactgt tcgagcattt ttccaatacc ggcgagctcg atccggcctc
    16561 cgacgtgccg ttccgcgccg cgatgaattc gatccacatc ggcaccacca tgctcggccg
    16621 ctgcgatggc accttcacca cggtgcggcg cgaacgccgc caggtgctgg agaccaatga
    16681 cgaccgctat tgcctcgcgc gcaacaccgg cgaccgcgaa tccctggtga tccatcgcgg
    16741 ccgcgccttc accctgcggc cgggcgcgat ggtgctcctg aaactcgacg agccgttctt
    16801 ttgcgccgat ggcgccagcc acaagcgttt taccaatgtg catctgccga tggcgacgct
    16861 gaaggccatg gtcgccggcg tcgatgacct ggtcgggcgc gaactggccg ggggcggcgc
    16921 gctgtcgctg gcgatggatt acagcgattt gttgctgcgg cataccggcg cggtcgacga
    16981 agccggaatg gcgatcgcgg cgcatctgac cgatctcgtg gctctcggcc tcggcgcccg
    17041 cggtgacctg gcgctcgcgg cccggcgcgg cggcctgcgc gccatcaggc tgaaggcagt
    17101 gctgatgatc ctggagaagc gttttaccga gccggatttt tccgcccaaa agctcgccac
    17161 cgcgaccggc ttgtcggagc gctatgtcaa cgaattgctc tacgaggccg gcgccagttt
    17221 cacgatgcgg ctgaacgagt tgcggctgcg caaggcggcg gagttgctga cgcgcggggc
    17281 tgagggccgg atcagcgaca tcgcattcga ttgcggcttt aacgatctgt cctatttcaa
    17341 ccgctgcttt cgccgccgct ttgggctgac gccctcggcg gcgcgcgggc gatagcgggt
    17401 gagataaagc gggatgggta accctcagcg tcatcccggc gaaggccggg atcccagcgt
    17461 gaacgcaatt gcgctcatcg cggcggcgcg gactcacgtc atccgcagtg tggtcgatgc
    17521 cttccgcaac aattgacgcc agcgatatgg gtcccggcct tcgccgggac gacgtggggg
    17581 agtgaaggtt cgccgggacg acatggggag tgacagcgtg acgcaacttg cgcaggcttc
    17641 gcgcgttacc atctgatggc gatgtcgaac caccagatgt actcccgaac caccgcgcgc
    17701 tcgcaagggc cgtttcgcgg atcgaagacg cacggcccga ttattgacca ggaggggcgc
    17761 gaaatccccg cagaggcgct tggtccgcag gttcgcggct ttcagttcga tttcgggaat
    17821 tccaccgcca atccgttcgc caatctcacc cgcgagcagc ggctggcgcg gctcgatgcg
    17881 gtggcgaagc ttctggacat tgccttcatc gtgcccggca ccaacatccg ctacggcatc
    17941 gatggcatca tcgggttgat cccggtggtc ggcgatatca ttgcgaccgc gctctcgctg
    18001 tggctggtgc gcgaggcacg cgcgctcggc gcgccctggc atgtcacggc gcggatgctg
    18061 ggcaatgtcg cggtgcaagg cgtgatcgga acggtgccgg ttgccggcga tgcgttcgac
    18121 gtgctgttcc gcgccaacat gcgcaacgcg cggctgctgc gacgctggat ggacaggcag
    18181 ccgcgctagc acgcaaacaa cctggagagc ggtgcacgag gttgcgtcga cagcacccga
    18241 attgcttcgc aattcgacct ttccccgcgg gagaggtgag gctaaatgcg aggcacgcac
    18301 caaatttgaa acgattgatc agacgagggc aagcggcgcg agcgtgacgc tgcgcgcaaa
    18361 taaaaaccgc gcaaacaaat gcccccggac gtgatccgag ggcattcatt taatgtgaga
    18421 gattacgcgg cgtcggcgtc ggcgtcgttg ctggcagggg cgggctcggg cgcacggggg
    18481 cggcgcggca gcggaaaatc ctggctttca tagagcgtgc ggatgccgct ttgatcgaag
    18541 cgggcttcgt caacctgcag ataggcgccg ttcagcgaat ccgccggcaa ttcctcgatc
    18601 gcgaactgca ccgcggcggc agcggtgtcg aagcggcgat aggcgaaacc cgcccgcttc
    18661 ttcttgcgga tcgcggcggg gaacaattcg gcagcggtgt tgtagctgaa ggaagccatg
    18721 gtcagtgacc tttatttttt ggcggagcgg acgacgatga aagggcgctg gcccgccatt
    18781 cggcggcgcg ccggttcgca tcatttcagg acagacccca atataagctc gtttgacaga
    18841 aatgcgactc ctgtcgagcc tgataatgaa tccgcgcgcc ccgccaggcg gcgcgaaata
    18901 tgtcctgtta tttcaatggc ttgagtggtc tagagcttgc caagcagcag caggatgagc
    18961 agcactacca gaataacgcc gaaaaggccc attccggagt ggccgtagcc gtagccgtag
    19021 ccgccgaacc ggccgctgaa gccacccagc aggaaaataa tcaggatgat gacaagtatc
    19081 agtccaagcg acatggcatt ctccccaacg ggcggccggc gaatggtcat ccgccgtccc
    19141 gccacccgga acaaagcata accccgcatt tggttccatc ggaggaacct ggaacgtcaa
    19201 tccgagtgtg cgaaaaaccg gcgcagggcc tgcgcagtga gttcgggcgc ggcttccggg
    19261 aagaaatgcc cacccttgag cgcgtatccc ctgaccttgc cggcccattg gcgccacagc
    19321 gtgaccggac cctcggcata ccagctgtcg agtggtcctt gcgcgctcca tattgcgagc
    19381 accggcgctc ggattttgcg ctccgcgtcg cgatcggcac ggtcatgcgc gcgatcgatg
    19441 ctggcggcgg cgcgatattc ctcgcaaatg gcatgggcgt ggccgatgtc gcgcagcgcc
    19501 tcgatatagg ctgcgcggac ttccagcgga aaagcttgcg acggcgaacc ccatccgccc
    19561 agcgcattat cgatgatggc ctccgccgac gtagcaagga tcgtttcggg aagcggctcg
    19621 ggctgtgcca gcagcgacca tggccaatag ccaagcgtga agtcggcatc ggcacgctcc
    19681 cacgcggtct caatgggaag gatgtcgagc accgcaagcc gctcgatacg gtccggatgg
    19741 tccagcgcca tgcgataggc gacgcggccg ccgcgatcgt gcccggcaac cgaaaagcgt
    19801 gtaaatccaa gccgctccat cacggtcacc atatcccggg ccatggcccg cttcgcataa
    19861 ggcgcatgat cgggggcgga tgccgggcag ccgctttgtc cgtagccgcg cagatcggca
    19921 cacacgacag tgaactcgtg cgccagcagt ggcgcgacgc cgcgccacat cacatgcgtc
    19981 tgcggaaaac cgtgcagcaa tagcagcggc gtgcccgatc cggaaatccg cacgaagatg
    20041 ctggtttcgc cggtctcgat gtgggctgat gtgaaatctt gcatgacgcc ggacaacgca
    20101 tgccaaaccg cgtgcgttcc accgctcttc cccctttgtg gaggagagtg gcttcgatgc
    20161 gaagcatcga agccgagtga gggattccgg tcgtcgataa acaccgcaac ccctcagccg
    20221 tcgcggaccg cgtccgcgac acctttcacg gacaagtctg tgcggtctgc cgaggcggag
    20281 cgatccgggt gatttgcccg acgggcggtc cggccgatgt cgtgtccagt gcccgcgtca
    20341 aaaatatttc gcttcacccg tcgggcaaat catctctaca agttcgcgcc atcccacccc
    20401 accagagggg cgtatcgcga tcgtcacgga cgcggggagg gatgcggtgg acgcggcagc
    20461 gttttgcgcg cgatgggat
//
LOCUS       DATNOI010000064         9768 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2145241,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000064 DATNOI010000000
VERSION     DATNOI010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9768
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2145241"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VN968_02205"
     CDS             <1..364
                     /locus_tag="VN968_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045861.1"
                     /note="catalyzes the reaction of formaldehyde with
                     glutathione to form S-hydroxymethylglutathione; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde-activating
                     protein"
                     /protein_id="HXL28093.1"
                     /translation="PRDSVTVLENGDKLHVIDASAAIQRHACKVCGTHMYGRIENKAH
                     PFYGLDFIHPELFQEAGSAAPGFAAFVSSVIESGVKPDEMPGIRSRLKELGLEPYDCL
                     SPALMDAIATHVAKSKAA"
     gene            475..1320
                     /gene="fghA"
                     /locus_tag="VN968_02210"
     CDS             475..1320
                     /gene="fghA"
                     /locus_tag="VN968_02210"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HXL28094.1"
                     /translation="MTIETVSLNRSHDGAQGVYKHISRETGTGMTFSVYVPPHPDGAK
                     LPVVWYLSGLTCTHVNVTEKGEFRKACSELGLIFVAPDTSPRGEGVPGDPAGVYDFGL
                     GAGFYVDATQAPFARNYRMWSYVTEELPELIAEHFPADSKRQSILGHSMGGHGALTVA
                     LRHPDRYRAASAFSPIVAPSQVPWGIKALGGYLGDSREAWRKHDTVALIEDGARFSDF
                     LVDCGDADPFLVEQLRLELLERACAKAEIPLVLRLHSGYDHSYYFISTFMEDHLRWHA
                     ARLQT"
     gene            complement(1337..1810)
                     /locus_tag="VN968_02215"
     CDS             complement(1337..1810)
                     /locus_tag="VN968_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3280 domain-containing protein"
                     /protein_id="HXL28095.1"
                     /translation="MASVPARADPPKVAVFDLELVDTSLQGEVDGPRTDEQRRLMRTG
                     DQLALAESGKFAVLDIAPVIAAAHASNLQSCGGCDVHYAQQLGADFAITGVVQKVSNL
                     ILNMKVYIRDAHTGQLVTSMSVDLRGNTDETWFRAASYLLRNRLLAPNYGAPQPQ"
     gene            1979..3163
                     /locus_tag="VN968_02220"
     CDS             1979..3163
                     /locus_tag="VN968_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL28096.1"
                     /translation="MIRLLAGAIGLGVLAGSALAADPVEIGIGYLHRGGVKPTLSLAE
                     QPAENDGLAGARLAIEDNNTTGKFLNQHFTLEELRLKDGDDAAKAAAALADRNIGFII
                     ADLPADALLKAADAVRDRGTVLFNAGSIDDRLREQDCRANVIHAAPTRSMLADGLAQY
                     LVWKQWKRWLLVVGSHEEDRLFAEVLRRAASRFGAKIVQERVFEDTGGARRTDSGVTL
                     IQRQMPVFTQQAPTYDVLVAADESEVFAAYLPYRTWDPRPVAGSAGLVPKSWDAAQDQ
                     WGAVQMQNRFLKLNSRHMTALDMQAWTAARMIGEAASRANSANPKSLLDLLKGPDFSV
                     AAFKGQRLTLRDWNLQLRQPILLADGRMVVSVSPQEGFLHQVSELDTLGVDRPETKCR
                     LH"
     gene            3181..4152
                     /locus_tag="VN968_02225"
     CDS             3181..4152
                     /locus_tag="VN968_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YVTN family beta-propeller repeat protein"
                     /protein_id="HXL28097.1"
                     /translation="MWRRCLLAGTAVWLAAATPAAAVMAYVSNEKSNTVSVIDTDTWA
                     VTKTIKVGQRPRGIEFTRDGKFVMVAVGDDDTIQVIDATTQQVVDTLPSGPDPELFVQ
                     DAAGKVLYVANENDNTVTVIDLEKRVRVGDVQVGVEPEGMAISPDGKILINTSETTNM
                     AHFIDTATREIVGNVLVDSRPRFAEFKRDGSELWVSSEIGGTVSVIDPAKHEVTGKIT
                     FSIPGLRREAIQPVGIGMTKDGKTAFIALGPANRIAIVDAASHQVTKYLLVGQRVWHM
                     AFTPDEKYLLVTNGVSNDVSIIDVVAQKVIKTIQVGELPWGITIAQP"
     gene            4218..4964
                     /locus_tag="VN968_02230"
     CDS             4218..4964
                     /locus_tag="VN968_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28098.1"
                     /translation="MPALSIDSVSHSYGARRALMDINFTVAPASFTALLGLNGAGKST
                     LFSLVTRLFGIQAGRIGIFGHDIGLAPGEALRLLGVVFQPRTLDLDLSVMQNLLYHAA
                     LHGIDRREARLRAGEVLARIGLADRAGSKVRDLSGGQMRRLEIARALLHRPRLLLLDE
                     ATVGLDVKARADILGHVRRLVAEQDIGVLWATHLFDEIIPSDDLVVLHQGRVLARGPV
                     ARIIADTGAHDINASFKRLTETAESGNSSP"
     gene            4961..5809
                     /locus_tag="VN968_02235"
     CDS             4961..5809
                     /locus_tag="VN968_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL28099.1"
                     /translation="MSSTIIKDQPTGFSLAQYVTCLNGIVWREGLRFLHQRERFVSAL
                     VRPLVWLFIFAAGFRQVLGISIIPPYETYIPYEVYIAPGLMAMIQLFNGMQSSLSMVY
                     DREMGNMRTLLVSPLPRWYLLFCKLLAGTAVSLLQVYAFLLIAWFWEIEPPPLGYLTV
                     LPALVLSGLMLGSLGMLISSGIKQLENFAGVMNFVIFPMFFASSALYPLWRVQEGSPM
                     LYYVCLFNPFTHAVELIRFALYGQMNWVALAVVGGCTAAFMIGAVFAYDPSRGLSRRG
                     PAGGET"
     gene            5806..6420
                     /locus_tag="VN968_02240"
     CDS             5806..6420
                     /locus_tag="VN968_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28100.1"
                     /translation="MRWRIMICALLAVAGSTIPCFAADPRYPDWPCAQAKVPEVSIAA
                     VWAGPPLDDVASNWKDDAKVRALLPRLAARRTPLDEAQKTITEFLAGSTAQKTEAGKL
                     LFAGLFDTLNAQRSQVMSGLERVTRKQREAADRIRADTLTLQALQDASPPDQAKIDEL
                     SNQLIWETRIFEDRRRVIKFVCEVPTAIDQRLFALGRVIQQEME"
     gene            complement(6447..7019)
                     /locus_tag="VN968_02245"
     CDS             complement(6447..7019)
                     /locus_tag="VN968_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated CXXCW motif
                     protein"
                     /protein_id="HXL28101.1"
                     /translation="MRKLASLITVALALVVPAHAQENAPEPEGYRTDNYRAPVPATLA
                     GARVLSTAEAEAIWRDKAAVFIDVLPRPPKPQGLPAGTVWRDKPRLNIPGSIWLPDTG
                     YGTLAAATEDYLRHGLERASGGNHARLLVIYCQADCWMSWNAAKRVLSYGFPNIAWYP
                     EGTDGWQRANLPVAESQPEPRAGEEMSSPQ"
     gene            complement(7019..7846)
                     /locus_tag="VN968_02250"
     CDS             complement(7019..7846)
                     /locus_tag="VN968_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXL28102.1"
                     /translation="MLTDREVLRAQTPDSTDLSIELVDPKVLRVCADPRNLPFSNEKG
                     EGFENKLGELFAEKLQKKLDYMYFPQATGFVRMTLAAHRCDVIMGFPQGDDLVQGTNP
                     YYRTAYALVAKQGGGLDDVATLEDERLKGKHIGIVAGTPPATNMAVNGLMANAKPYPL
                     MIDTRFDSSAAAMIKDLTSGEIDAAVLWGPMAGYYAKQANPPLHVTPLVKETTGPRLA
                     YRIGMGVRPADQNWKRQLNRLIQENQPAINKILLDFGVPLLDDNDRPITAETTTKQP"
     gene            complement(7989..8477)
                     /locus_tag="VN968_02255"
     CDS             complement(7989..8477)
                     /locus_tag="VN968_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome, methanol metabolism-related"
                     /protein_id="HXL28103.1"
                     /translation="MALVASGGTVFADGAVDPTAIKSEDGKYLDKEGSPTFKVASDGT
                     VDWYTYSGYRRYHSECHVCHGPDGMGSTYAPALKDSLKTMSYPDFLGAVASGRKNVSS
                     SQENVMPAFGDNPNVACYMDDLYVYLRARASDAVGRVRPAKHEDKPPAAQQAEDSCMG
                     KR"
     gene            complement(8587..>9768)
                     /gene="xoxF5"
                     /locus_tag="VN968_02260"
     CDS             complement(8587..>9768)
                     /gene="xoxF5"
                     /locus_tag="VN968_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthanide-dependent methanol dehydrogenase
                     XoxF5"
                     /protein_id="HXL28104.1"
                     /translation="GKQVWRAFSEGPDDQIMVDPEKTTVLGKPVGKDSSLKTWQGDQW
                     KIGGGCTWGWMAYDPGLNLVYYGSGNPSTWNPKQRPGDNKWSMTIFARDADTGVAKWV
                     YQMTPHDEWDYDGVNEMILSDQQVGGAARKLLTHFDRNGLGYTLDRATGELLVAEKYD
                     PKVNWTSGVDMDKNSPTYGRPKVLDQYSTDKQGEDHNNKGICPAALGTKDEQPAAYSP
                     ETQLFYVPTNHVCMDYEPFKVSYTAGQPYVGATLSMYPPPGESNMGNFIAWDGKTGKI
                     AWSNKEQFSVWSGALATAGGVVFYGTLEGYLKAVDAKTGKELYKFKTPSGIIGNVTTY
                     EHGGKQYVAVLSGVGGWAGIGLAAGLTDPTAGLGAVGGYAALSNYTALGGNLTVFALP
                     Q"
BASE COUNT         1723 a         3081 c         3047 g         1917 t
ORIGIN      
        1 gccacgcgac agcgtgaccg tgctcgagaa cggcgacaag ctgcacgtca tcgacgcctc
       61 ggcggcgatc cagcggcatg cctgcaaggt ctgcggcacg cacatgtacg gccgcatcga
      121 gaacaaggcg cacccgttct atgggctcga cttcatccat cccgaactgt tccaggaagc
      181 cggctcggcg gcgccgggat tcgcggcgtt cgtgtcgtcc gtgatcgagt ccggcgtcaa
      241 gccggacgaa atgcccggca tccgctcgcg tctcaaggaa ctcgggctcg agccctatga
      301 ttgcctgtcg ccagccctga tggatgcgat cgccacccat gtggcgaaat ccaaagcggc
      361 ctgatccggc ggccttgcgt aagcttccgc tggtggttat caccaccggc ggagccgcga
      421 tcacgtcaga tccgttgcca tcttcgcgtc gttgctgcgc tggagcctaa catcatgacg
      481 atcgaaaccg tttctctcaa tcgatcgcat gacggtgcgc agggtgtcta caagcacatc
      541 agccgcgaga ccggaaccgg catgaccttt tcggtctatg tgcccccgca tccggatggc
      601 gcgaagctgc cggtggtctg gtatctatcg ggtctgacct gcacccacgt caacgtcacg
      661 gaaaagggtg aatttcgaaa ggcctgctcc gaactgggat tgatctttgt tgcgccggac
      721 accagtccgc gtggcgaagg cgtgcccggc gatcccgccg gcgtctatga tttcgggctt
      781 ggcgccggct tctatgtcga tgccacgcaa gcgccgtttg cccgcaatta ccggatgtgg
      841 agctatgtga ccgaagaatt gcccgaattg atcgccgagc attttcccgc cgattccaaa
      901 cggcaatcca ttctcggtca ttcgatgggc ggccacggtg cgctgaccgt cgcgctgcgc
      961 caccccgatc gttaccgcgc cgccagcgct ttttcgccaa tcgtggcgcc ctcgcaagta
     1021 ccttggggca tcaaggcgtt ggggggttat ctcggcgata gccgggaagc gtggcgcaag
     1081 catgacaccg tcgcgctgat cgaggacggt gcccggtttt ccgattttct cgtcgattgc
     1141 ggcgatgccg acccgttcct ggtggaacag cttcgtctgg aactgctcga gcgcgcatgt
     1201 gcgaaggccg aaatccccct cgtccttcgt ctccacagcg gctacgacca cagctactac
     1261 ttcatttcca ccttcatgga agatcatctg cgttggcatg ccgcgcggtt gcaaacctag
     1321 gcgctcgccc gaaccgttat tgtggttgcg gcgcgccata gttgggcgcg agcagccggt
     1381 ttcgcagcag atagctcgcc gcgcggaacc aggtttcgtc ggtattgccg cgaaggtcga
     1441 cgctcatcga cgtgacgagc tgtccggtat gggcgtcccg aatgtagacc ttcatattca
     1501 gaataaggtt cgaaaccttt tgaaccacgc ccgtgatcgc gaaatcagcg ccgagctgtt
     1561 gtgcgtaatg cacgtcgcaa ccgccgcagg actgtagatt gctggcgtgc gccgcagcaa
     1621 tgaccggcgc gatgtcgagg accgcaaact ttccggattc cgccaatgcc agctgatcgc
     1681 cggtgcgcat tagccgccgc tgttcgtcgg tccgcggccc atccacttcg ccctgaaggc
     1741 tggtgtccac cagttcaaga tcaaacacgg cgactttggg tggatcggcg cgcgccggta
     1801 ctgacgccat cagcagcaac gcgacgggag ccgagagata tttcatgatc gggatccgcg
     1861 aaatccgcgg cggcgcggcg acaaaatgtc ggttgcatcg tcggacaatt taaccatggt
     1921 tcaagccggg agtccctcca tcgccggttc cgtcgaggag aaatgtcagg gaggaaacat
     1981 gatccgattg ttggccggcg cgatcggctt gggtgtgctg gctggcagtg cgcttgcggc
     2041 cgatccggtc gaaatcggca tcggctatct tcatcgcggc ggtgtcaagc cgacactgtc
     2101 gctggccgag cagccggccg aaaacgacgg cctggcgggc gcccgcctcg cgatcgagga
     2161 caacaatacc accggaaagt tcctgaatca gcatttcacc ctggaagagt taaggctcaa
     2221 ggacggcgat gacgcagcca aggcggccgc tgcactcgcc gatcgcaaca tcggtttcat
     2281 cattgccgat ctccccgcgg acgccctcct aaaggccgcc gacgcggtcc gcgaccgcgg
     2341 aaccgtgttg ttcaacgccg gctcgatcga cgatcgcctg cgcgaacagg attgccgcgc
     2401 caacgtgatc catgcggccc cgacgcgctc gatgctggcg gatggtctcg cccaatatct
     2461 tgtctggaag cagtggaagc gctggctgct ggtcgtcgga tcgcacgaag aggataggct
     2521 gtttgccgaa gtgttgcggc gcgctgcctc gcgcttcggc gcgaagatcg tccaggagcg
     2581 ggtattcgag gataccggcg gcgcgcgccg caccgatagt ggcgtgacgt tgatccagcg
     2641 tcaaatgccc gtgttcaccc agcaggcccc cacttatgat gttcttgtcg cagccgatga
     2701 gagcgaggtg tttgcggcgt atctgcccta ccgaacctgg gacccgcggc cggtggccgg
     2761 atccgctggc cttgttccga aaagctggga cgccgcccag gatcagtggg gcgcggtgca
     2821 gatgcaaaac cgctttctga aattgaattc gcggcatatg acggcgctgg acatgcaggc
     2881 atggaccgcg gcgcgcatga tcggcgaggc ggcgtcgcgc gcgaattctg cgaatccaaa
     2941 atcgttgctc gaccttctca aggggccgga tttttccgtc gccgccttca agggccaacg
     3001 gctgacgctg cgagactgga acctgcagct gcgtcagccg atcttgctgg cggacggacg
     3061 catggtggtt tcggtctcgc cgcaggaagg tttcctgcac caggtctcgg aactcgatac
     3121 gctcggcgtc gatcgtcccg aaaccaaatg caggctgcat tgaaaatgga gaggttgcgg
     3181 atgtggcgtc gttgtctcct tgccggaact gccgtctggc ttgcggccgc cacgcccgcc
     3241 gcggcggtca tggcctatgt gtcgaacgag aaaagcaaca cggtgtcggt gatcgatacc
     3301 gacacatggg ccgtcaccaa gaccatcaag gtcggccagc ggccgcgtgg catcgagttt
     3361 accagggacg gcaaattcgt gatggtcgcg gtcggcgatg acgacaccat tcaggtgatc
     3421 gacgctacga cccaacaggt ggttgacacc ctgccctccg gtcccgatcc cgaactgttc
     3481 gtgcaggatg ccgccggcaa ggtgctttat gtcgccaacg agaacgacaa cacggtcacc
     3541 gtcatcgatc tcgaaaagcg cgtgcgggtc ggcgacgtgc aggtcggcgt cgagcccgag
     3601 ggcatggcca tcagcccgga cggcaagatt ctgatcaata cgtcagagac caccaacatg
     3661 gcgcatttca tcgacaccgc gacgcgcgag atcgtcggca acgtgctggt cgattcgcga
     3721 ccgcgctttg ccgaattcaa gcgcgacggc tccgaactgt gggtgtcctc ggaaatcggc
     3781 ggcacggtgt ccgtcatcga cccggcgaaa cacgaggtta ccggcaagat tacattcagc
     3841 atcccgggcc tgcggcgcga ggccatccag ccggtcggca tcggcatgac caaggatggc
     3901 aagaccgctt tcatcgcgct tggcccggcc aaccggatcg cgatcgtcga tgccgccagc
     3961 catcaggtca ccaaatacct tctggtcggc cagcgggtct ggcacatggc gttcaccccg
     4021 gatgaaaaat acctgctggt gaccaacggc gtatccaacg acgtctcgat catcgatgtg
     4081 gttgcgcaga aggtgatcaa gaccatccag gtcggcgagt tgccctgggg catcacgatt
     4141 gcgcagccat gacggcaacc gacgccccca tcgccggcca aattccgccg gacagtcgct
     4201 gctgcgatcc gggcgaagtg ccggcgctgt cgatcgacag cgtcagccat tcctatggtg
     4261 cccggcgcgc gctgatggac atcaacttca ccgtcgcgcc cgcaagcttc acggcgctgc
     4321 tcggcctcaa cggcgccggc aaaagcacgt tgttctcgct ggtcacgcgg ctgttcggca
     4381 tccaggccgg acgcatcggc atcttcggcc acgacatcgg tctggcgccc ggcgaagcct
     4441 tgcggctgct gggcgtggtg ttccagccgc gcacgctcga cctcgatctc tcggtcatgc
     4501 aaaacctgct gtatcatgcc gccctgcacg gcatcgaccg gcgcgaggcg cgcctgcgcg
     4561 ccggtgaagt gctcgcgcgc atcggccttg ccgatcgcgc cggcagcaag gtgcgcgacc
     4621 tctcgggcgg ccagatgcga cggctggaaa tcgcccgcgc gctgttgcat cgccctcgcc
     4681 tgctgctgct cgacgaggca accgtcggcc tcgatgtcaa ggcccgcgcc gatatcctcg
     4741 gccacgtccg gcgactggtc gccgaacaag acatcggcgt attgtgggcc acccatttgt
     4801 tcgacgaaat catccccagc gatgacctcg tggtgctgca ccagggccgg gttctcgcgc
     4861 gcggcccggt cgcgcgcatc atcgccgaca ctggagctca cgacatcaac gcctcattca
     4921 agcgcctcac cgaaacggca gagtccggga atagctcccc atgagctcca ccatcatcaa
     4981 ggatcaaccg accgggtttt cgctcgcgca atatgtcacc tgcctgaatg gcatcgtgtg
     5041 gcgcgagggg ctgcgctttc tgcatcagcg cgaacgcttc gtctcggcgc tggtgcggcc
     5101 gctggtatgg ctgttcatct tcgccgccgg cttccgccag gtgctcggca tctccatcat
     5161 cccgccctac gagacctaca tcccctacga ggtctatatc gcgcccggat tgatggcgat
     5221 gatccagctg ttcaacggca tgcagtcctc gctctcgatg gtctatgacc gcgagatggg
     5281 gaatatgcgc acgctgctgg tgagcccgct gccgcgctgg tatcttctgt tctgcaagct
     5341 gctcgccgga accgcggtct cgctgctgca ggtctatgcc ttcctgctga tcgcgtggtt
     5401 ctgggagatc gagccgccgc cgttgggtta tctcaccgtc ctgcctgcgc tggtgctgtc
     5461 aggcttgatg ctcgggtcgc tcggcatgct gatttcatcc ggcatcaagc agctggagaa
     5521 ttttgcaggc gtgatgaact ttgtgatctt cccgatgttt ttcgcctcgt ccgcgcttta
     5581 tccgctatgg cgggtgcaag aggggagccc gatgctctac tacgtctgcc tgttcaatcc
     5641 attcacccat gcggtcgagc tgatccgctt cgcactgtac ggacaaatga actgggtcgc
     5701 actggcggtg gttggcggct gcacggcggc cttcatgatc ggcgcggtgt tcgcctatga
     5761 tccatcgcgg ggcctcagcc gccgcggacc cgccggaggc gagacatgag atggcgcatc
     5821 atgatctgcg cgttgctcgc ggtcgccgga tcgacaatac cttgcttcgc cgctgatccg
     5881 cgctatccgg actggccgtg cgcccaggcc aaggtgccgg aggtctcgat cgccgcggta
     5941 tgggcgggtc cgccgctcga cgacgtcgca agcaattgga aggacgacgc caaggtgagg
     6001 gcattgttgc cgcgcctggc cgcaaggcgc acgccgctcg acgaagctca gaagacgatc
     6061 acggaatttc ttgcgggttc cacggcgcaa aagaccgagg cgggcaagct attgttcgca
     6121 ggcctgttcg acacgctcaa cgcgcagcgt tcccaggtca tgagcgggct cgaacgggtg
     6181 acccgcaaac agcgcgaagc cgcggaccgg attcgtgccg acacgctgac gctgcaagcc
     6241 ctgcaggatg cctccccgcc cgatcaagcc aagatcgatg agctgagcaa tcagctgatt
     6301 tgggagacac ggatcttcga ggaccgccgg cgcgtgatca aattcgtctg cgaggtaccg
     6361 accgcgatcg accagcggct gttcgcgctc ggccgcgtga tccagcagga gatggaatag
     6421 cgcgcgcggt atgccgccgg ccgctctcac tgcggcgatg acatctcctc acctgctctg
     6481 ggctccggct gcgattccgc caccggcagg ttggcgcgct gccaaccgtc agtgccttcg
     6541 ggataccagg ctatattggg aaagccatag gacaacaccc gctttgctgc gttccaagac
     6601 atccagcagt cggcctggca gtagatcacg agcagccttg catggttgcc gccggaagca
     6661 cgctcaagcc cgtgccgcag atagtcttcg gtcgctgccg cgagcgtccc gtagccggtg
     6721 tccggcagcc agatgctgcc ggggatgttg agccgcggtt tgtcacgcca gaccgtgccc
     6781 gcgggaagac cttgcggttt cggcggacgt ggcagcacat cgatgaaaac agccgccttg
     6841 tcgcgccaga tggcttcggc ctcggcggtc gacagcacgc gggcgccggc gagcgttgcg
     6901 gggacaggcg cgcggtaatt gtccgtgcgg tatccttccg gctcgggagc gttttcctgc
     6961 gcatgggccg gcaccaccaa agcaagcgca acggtgatca ggctcgcgag cttccgcatc
     7021 atggctgctt cgtcgtcgtc tccgcggtga tcggcctgtc attgtcgtca agtaacggca
     7081 cgccgaagtc gagcagtatc ttgttgatgg ccggctggtt ctcctggatc aggcggttga
     7141 gctgccgttt ccagttctgg tcggccggtc gaacacccat gccgatgcga taggcgagcc
     7201 gcggtccggt ggtttccttg acgaggggtg tgacgtgaag cggcgggttg gcctgcttgg
     7261 cataatagcc agccatcggc ccccacagaa cggcggcatc gatctccccg gaggtcagat
     7321 ccttgatcat tgccgctgcc gaggaatcga agcgggtatc gatcatcagc ggataaggct
     7381 tggcattcgc catcagacca ttgaccgcca tgttggtggc aggcggcgtg ccggcaacga
     7441 tgccgatatg tttgcccttg agacgctcgt cctcaagggt cgccacgtca tcgaggcctc
     7501 cgccctgctt ggcaaccagc gcgtaggccg tgcggtaata gggattggtg ccctgaacca
     7561 gatcgtcgcc ttgcgggaaa cccatgatga cgtcgcaacg gtgggcggca agcgtcatcc
     7621 gaacgaaacc ggtcgcctga ggaaagtaca tgtaatcaag cttcttctga agcttctcgg
     7681 caaacagttc gccgagcttg ttctcgaatc cttcgccctt ctcgttggag aacggcagat
     7741 tgcgcgggtc ggcgcatacc cgcagcacct ttggatcgac cagttcgatc gacagatcgg
     7801 tgctatcagg cgtctgcgcc cgcaagacct cgcggtcggt cagcatcgcc gcaaaggcaa
     7861 caagtggaat caaaagccag cggtgcagcg cggcattgat catcgccgtc tttctcctgt
     7921 ttgatcgggt aattatccgg gacatcaatg agattcctgc cgctagcgcc tgacggtgcg
     7981 gcttcggatc atcgcttgcc catgcaggaa tcctcggcct gctgcgcggc gggaggcttg
     8041 tcttcatgct tcgcgggccg cacacgccct accgcgtcgc tggcgcgggc acgcagatag
     8101 acgtagaggt cgtccatgta gcaggcgacg ttcggattgt cgccgaaggc cggcatgacg
     8161 ttctcctgcg aactgctgac gttcttgcgg ccgctggcca ccgcgccgag gaagtctggg
     8221 tagctcatcg tcttgagtga gtccttcagc gccggcgcgt aggtcgatcc catgccgtcg
     8281 gggccgtggc agacgtggca ctccgagtga taacggcggt atcccgaata ggtgtaccag
     8341 tcgacggtgc cgtcggatgc gaccttgaac gtcgggcttc cttccttgtc cagatatttt
     8401 ccgtcctctg atttgatggc tgtcggatcg acagcgccgt cggcgaatac tgttcctcca
     8461 gatgccacga gggccatcgt ggcagccacg agccagattt tatgcaagtg cttttcctta
     8521 agcgttttga tgacagacca accggcgcgt gaagcatctc cacgcgccgg aacggtcctg
     8581 atcaggtcac tgcggcagcg cgaacaccgt caggttgcca ccgagtgcgg tgtagttgct
     8641 gagcgccgcg taaccgccga cggcgccgag acccgcggtc ggatcggtca gaccggccgc
     8701 aagcccgatg ccggcccagc cgccgacgcc ggacagcacc gccacatact gtttaccgcc
     8761 atgctcgtag gtcgtgacgt tgccgatgat gccggacggg gtcttgaact tgtagagttc
     8821 cttgcccgtc ttggcgtcga ccgccttgag atagccctcg agcgtgccgt agaacaccac
     8881 gccgccggcg gtggcgagcg cccccgacca gaccgagaac tgctccttgt tcgaccaggc
     8941 gatcttgccg gtcttgccgt cccacgcgat gaagttgccc atgttggact cgcccggagg
     9001 cggatacatc gacagcgtcg ccccgacata gggctggccc gcggtgtagc tcaccttgaa
     9061 cggctcgtag tccatgcaga cgtggttggt cggcacgtag aacagctgcg tctccggcga
     9121 ataggccgcc ggctgctcgt ccttggtgcc aagcgccgcc gggcagatcc ccttgttgtt
     9181 atggtcctcg ccctgcttgt cggtcgaata ttgatcaaga accttgggac ggccataggt
     9241 cggggagttc ttgtccatat cgacgccgga ggtccagttg accttcggat cgtacttttc
     9301 ggcgaccagc aattcgccgg tggcgcggtc gagggtatag ccgagcccgt tgcggtcgaa
     9361 atgggtcagc agcttgcgcg cggcgccgcc gacctgctga tcactgagga tcatttcgtt
     9421 gacgccgtca tagtcccatt catcatgggg cgtcatctgg tagacccact tggccacccc
     9481 ggtgtcggca tcgcgcgcga agatggtcat cgaccacttg ttgtcgcccg gacgctgctt
     9541 cggattccag gtcgagggat ttcccgagcc gtaatagacg aggttcaggc cgggatcata
     9601 ggccatccag ccccaggtgc agccgccgcc gattttccac tggtcgccct gccaggtctt
     9661 gaggctggaa tccttgccga ccggcttgcc gagcaccgtg gttttctccg gatcgaccat
     9721 gatctggtcg tccggtcctt cggagaacgc ccgccagacc tgcttgcc
//
LOCUS       DATNOI010000065         2444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1194100,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000065 DATNOI010000000
VERSION     DATNOI010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194100"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="VN968_02265"
     CDS             <1..1639
                     /locus_tag="VN968_02265"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HXL28105.1"
                     /translation="LGFGASLISIFARLGGGIFTKGADVGGDLVGKVEAGIPEDDPRN
                     PATIADNVGDNVGDCAGMAADLFETYAVTTVATMVLAAIFFRTAGADTLLRMMTYPLA
                     IGGICIITSIIGTFFVRLGASQSIMGALYKGLIVTGVLSLAGVALVTHLLVGFGSVPG
                     QSYSGTSLFICGVVGLIVTGLIIWITEYYTGTNFRPVQSIAKASVTGHGTNVIQGLAI
                     SLEATALPAIVIIAGILTSYKLAGLFGIAVAVTTMLSLAGLIVALDAFGPVTDNAGGI
                     AEMAGLPKEVRKSTDALDAVGNTTKAVTKGYAIGSAGLGALVLFAAYNEDLKFFIANA
                     ASNPYFQGVQLDFSLNNPYVVVGLLFGGLLPYLFGALGMTAVGRAASAIVEEVRRQFR
                     EKPGIMQGTDKPDYGRAVDLLTKAAIKEMIVPSLLPVLSPIVVYFLIYWIAGGGSIGK
                     SAAFSAVGAMLLGVIVTGLFVAISMTSGGGAWDNAKKFIEDGHYGGKGSEPHKAAVTG
                     DTVGDPYKDTAGPAVNPMIKITNIVALLLLAVLAHWR"
     gene            complement(1717..2130)
                     /gene="bamE"
                     /locus_tag="VN968_02270"
     CDS             complement(1717..2130)
                     /gene="bamE"
                     /locus_tag="VN968_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HXL28106.1"
                     /translation="MAAARVAVTVAFGILLSGCFSETYQRGYIVPEGALEQLPIGSTQ
                     EQVLIVLGTPSTVATVSGEAFYYISQRAERSIGFMPQRVVDQRVVAVYFDKNRRVERL
                     ANYGLKDGKVFDFVSRATPTGGKDVAYLNALFKIF"
BASE COUNT          412 a          784 c          748 g          500 t
ORIGIN      
        1 actcggcttc ggcgcgtcgc tgatctcgat cttcgcgcgt ctcggcggcg gcatcttcac
       61 caagggggcg gacgtgggcg gcgacctcgt cggcaaggtc gaggccggca ttcccgagga
      121 tgatccgcgc aatcccgcga ccatcgccga caatgtaggc gataatgtcg gtgactgcgc
      181 cgggatggcg gccgacctgt tcgagaccta cgccgtgacg acggtcgcta ccatggtgct
      241 ggcggcgatc ttttttcgca ccgccggcgc cgacacgctc ctcaggatga tgacttatcc
      301 ccttgccatc ggcggcatct gcatcattac gtccatcatc ggcacgttct tcgtcagact
      361 cggcgcgagc cagtcgatca tgggcgcgct ctacaagggc ctcatcgtca ccggcgtgct
      421 ctcgctcgcc ggcgtagcgc tggtcaccca tctcctggtg ggcttcggct ccgtgccggg
      481 gcagtcctat tccggtacat ctctgttcat ctgcggcgtg gtcggcctca tcgtgaccgg
      541 gctcatcatc tggatcaccg agtactatac tggtacgaat ttccggccgg tgcagtcgat
      601 cgccaaggcc tcggttacgg gccacggcac caacgtcatc caaggtttgg ccatttcgct
      661 ggaagcaacg gcgctgcccg cgatcgtcat catcgccggc atcctgacca gctacaaact
      721 cgccggcctg ttcgggatcg cggtcgccgt gaccacaatg ctttcgctcg ccggcttgat
      781 cgtggcgctc gatgcgttcg ggccggtgac cgacaacgcc ggcggtatcg cggaaatggc
      841 ggggctgccc aaggaggtgc gcaagtcgac cgacgccctc gacgccgtcg gcaacacgac
      901 caaggcggtg actaagggct atgccattgg ttcggccggt ctcggcgcac tcgttctgtt
      961 cgcggcttac aacgaggact tgaagttttt catcgccaat gcggcgagca atccgtactt
     1021 ccagggcgtg cagctcgatt tctccttgaa caacccctat gtcgtggtgg gactcttatt
     1081 cggcggcttg ctgccgtatc tgttcggtgc gttgggcatg acggcggtcg gccgcgcggc
     1141 gtctgcgatc gtcgaggagg tgcgccggca gtttcgtgag aagcccggca tcatgcaggg
     1201 caccgacaag cccgattacg gccgtgccgt cgacctcctc accaaggcgg cgatcaagga
     1261 gatgatcgtt ccgtcgctgc tgccggtgct ttcgccgatc gtggtctatt tcctgatcta
     1321 ctggattgcg ggcggcggtt cgatcggtaa gtcagcggcg ttctccgctg tcggcgcgat
     1381 gctcctcggc gttatcgtca ccggcttgtt cgttgcgatt tcgatgactt cgggtggcgg
     1441 cgcatgggac aatgccaaga aatttatcga ggacggccac tacggcggca aaggttcgga
     1501 gccgcacaag gcggcggtga cgggcgatac cgtgggtgat ccctacaagg acacggccgg
     1561 tcccgccgtc aacccgatga tcaagatcac caatatcgtc gcgctcttgt tgctcgccgt
     1621 gctcgcgcac tggcgctgac gcctcccagt gggaggatgc gacgcgcgct gacgccgcgg
     1681 cacactgcgc cgcggcggtg cctcttcatc accggtctag aaaatcttga atagcgcgtt
     1741 gagataggcg acgtccttgc cgccagtcgg cgtggcgcgg ctgacaaaat caaagacctt
     1801 gccgtccttg agaccgtagt tcgcgagacg ctccactcgc cgattcttgt cgaaatacac
     1861 cgccacgacc cgctgatcga ccacccgttg gggcatgaag ccgatcgacc gctcggcgcg
     1921 ctgggaaata taataaaacg cctcgccact caccgtcgcg accgttgagg gcgtgccgag
     1981 cacgatcaaa acctgttctt gggtggaacc gatcgggagc tgctcgagcg ccccttcggg
     2041 cacgatgtag ccgcgctgat aggtctccga aaagcagccg ctgagcagga ttccgaatgc
     2101 caccgtcacc gcaacgcggg cggcggccat acggaacggg cgcgtcggtc cattcatgca
     2161 gctcgtgatc cccctgtcat ggtcgcaggc gctagcgcat tgatctcatc ggcgctatca
     2221 cgttttccct atcgagcccc tgcatccgac ctccgcggcc gagctaacgc gatgatattc
     2281 aagttgtttc gccgcacccc gcgggacaat accatcgcgt gtctctatgg cacgatcgtg
     2341 gcacaggcgc ggctgcccgc cttttaccaa atctatggcg tgcccgatac ggtcaacggc
     2401 cgactggaga tgatcatgct ccataccgtg ctgtttctcc gccg
//
LOCUS       DATNOI010000066         2135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2278161,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000066 DATNOI010000000
VERSION     DATNOI010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2278161"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1752
                     /locus_tag="VN968_02275"
     CDS             <1..1752
                     /locus_tag="VN968_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HXL28107.1"
                     /translation="PALLEPMFTLRTQNLIWTGGYALLIVLIGACAVLLLRSPATAYV
                     LAQTDETDAAPPPWSLRARWIFLAAVPSGLLIAVTAHISTDIAAAPLLWVLPLSLYLL
                     TWVLVFQSRPLLPHQSMLRLQPLAIAGVVVLLAVGGEQNLLLTLGGHQLCFFVIAMAC
                     HGELARTRPAAKYLTGFYVALSFGGMIGGLFAGLIAPYTFSWIAEYPILLALAALCRP
                     PGGNERFPRWSSWYWPFLAVLAIALIAPSWSGGEVTTWFGEYRVWVAGAVGLLSALLA
                     LGLNANRWKIFATVMLALVLIRAYPADKGRVETVRSFFGVHKILVTPHGQYHVLMHGT
                     TIHGAEKFQNNDGTPVTGRPEPITYYHEDGGIGQAIEAVRERKGAPLRVAVIGLGAGT
                     LACASKSGESWKFFEIDQTMVDTARDPKYFAYIQSCEPDLKPVIGDARLTFAREPDGL
                     YDLIIVDAYSSDAIPVHLATQQAMAIYKSKLAPQGAVLVHVSNRHLELSSVIVGIAEV
                     NNLKSWVYSEDTDRDDEYIFATSVVVCARKEADVGKLASSEDWALEEADENQRVWTDD
                     YSNVLGAVWRRLRNGEP"
     gene            1881..2108
                     /locus_tag="VN968_02280"
     CDS             1881..2108
                     /locus_tag="VN968_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HXL28108.1"
                     /translation="MPVNAEMKDHVYKILELVGSSEKSIEDAILNAITRASKTIRDMK
                     WFEVVQTRGHIEKGSVAHYQVTLRVGFTLEH"
BASE COUNT          388 a          648 c          678 g          421 t
ORIGIN      
        1 ccggcgctgc tggagccgat gttcacgctg cgcacgcaaa acctgatctg gaccggcggc
       61 tacgcgctgc tgatcgtgct gatcggggct tgcgcggtgc tgctgctgcg ctcgcccgcg
      121 acggcttatg tccttgcgca gaccgatgag accgacgcag caccgccgcc gtggagcttg
      181 cgcgcgcggt ggatctttct cgccgcggtg ccgtcgggcc tcttgatcgc ggtgaccgcg
      241 catatctcca ccgatatcgc cgcggcgccg ttattgtggg tgctgccgct gtcgctctat
      301 ctcctgacct gggtgctggt gttccagtcg cgcccgctgc tgccgcacca atcgatgttg
      361 aggctgcagc cgctggcgat cgcaggcgtg gtcgtgctgc tcgcggtcgg cggcgaacag
      421 aacctgttat tgacgctcgg cggccaccag ctttgtttct tcgtgattgc gatggcctgc
      481 catggcgagc tggcgcgtac ccgtccggct gcgaaatatc tcaccggttt ctatgtcgcg
      541 ctgtcattcg gcggcatgat cggcggattg ttcgcagggc taatcgcgcc ttatacgttt
      601 tcatggatcg cggaataccc gatcctgctg gcgctggcgg cgttgtgccg cccgcccggc
      661 ggcaacgagc gtttcccgcg ctggagcagc tggtactggc cgtttctcgc cgtgctcgcg
      721 atagcattga tcgcgccgtc ctggtcgggg ggcgaggtca cgacatggtt tggcgaatac
      781 cgggtctggg tggccggcgc cgtcggcctg ctctcggcat tgctggcgct cggattgaac
      841 gccaaccgct ggaaaatctt cgcaaccgtc atgctggcgc tggtgctgat ccgcgcctat
      901 ccggccgaca agggccgcgt ggaaaccgtg cgcagcttct tcggcgtcca caagatcctg
      961 gtgacgccgc acgggcagta tcacgtcttg atgcacggca ccacgataca cggcgccgag
     1021 aaatttcaga acaacgatgg cacgccggtc accgggcggc ccgagccgat cacctattat
     1081 cacgaggatg gcggcatcgg tcaggcgatc gaggccgtgc gcgagcgcaa gggcgcaccg
     1141 ttgcgggttg cggtgatcgg cctcggcgcc ggcacgctcg catgcgcctc aaagtccggc
     1201 gagagctgga aattcttcga gatcgatcaa acgatggtcg ataccgcgcg cgatccaaaa
     1261 tattttgcct acattcaaag ctgcgaaccg gatctgaagc cggtgatcgg cgacgcgcgg
     1321 ctgaccttcg ccagggagcc ggacggcctc tacgacctga tcatcgtcga tgcctattcc
     1381 tcggacgcaa tcccggtcca tctcgccacc caacaggcga tggcgatcta caaaagcaag
     1441 ctcgcgccgc agggcgccgt gctcgtgcac gtctcgaacc ggcatctgga gctgtccagc
     1501 gtgatcgtcg gcatcgccga ggtcaacaat ctcaagagct gggtctacag cgaggatacc
     1561 gaccgtgacg acgaatatat cttcgcaacc tcggtcgtgg tctgcgcgcg caaggaggcc
     1621 gacgtcggca agctggcgtc gtctgaggat tgggcgctgg aggaagccga cgagaaccag
     1681 cgggtctgga ccgacgatta ttccaacgtg ctcggcgcgg tctggcggcg gctgcgcaac
     1741 ggcgagccgt agcgcttcgc cgcagccata ttgccgggca gaaggagagg aacccgccgg
     1801 gtggagagcg cgttcacaat ggaattggct cacgatcgga ctaaaactct gcccgactga
     1861 caccacaacg gaggttcgcc atgccagtta acgctgaaat gaaggatcat gtttacaaga
     1921 tcctggagct cgtcggatct tccgaaaaaa gtatcgagga tgcgattctg aatgccatca
     1981 ctcgcgcatc aaagaccatt cgcgatatga aatggttcga ggtcgtgcaa acgagaggcc
     2041 atatcgaaaa gggatctgtt gcccattacc aggtcacgtt gcgtgttgga tttacgctgg
     2101 agcattagtt ttcggaaaaa cccacatccg accga
//
LOCUS       DATNOI010000067         5020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_348433,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000067 DATNOI010000000
VERSION     DATNOI010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348433"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VN968_02285"
     CDS             <1..1249
                     /locus_tag="VN968_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXL28109.1"
                     /translation="FVKWADRIEYPDAAPTLVSRAFQEMLSGRRGPVALEMPWDVFTQ
                     RAETGVAKPFDLFPAPQPDTERVKAAATLIASSKAPMIFVGSGAIHARDEILELAELI
                     DAPVVAFRSGRGIVSNAHELGLTMAAAYRLWPSTDLMIGIGSRMELPTMFRWPFRPDG
                     LKSVRIDIDPSEMRRFTPDAPVIADAQAGTRELLAAVRKAGYTKTSGRRSAIREASAA
                     ALREIQKVQPQMAYLNILREVLPHNAIVTDELSQVGFTSWYGFPVYEPRTFITSGYQG
                     TLGSGFPTALGAKVAHPDRPVVAITGDGGFMFGVQELATAVQFNIGVVTLVFNNNAYG
                     NVRRDQRERFDGRVVASDLVNPDFVKLAESFGVAAARVTSPETFRAALEKALAHGGPY
                     LIDIEVPKDSETSPWAFIHPAKP"
     gene            complement(1421..2074)
                     /gene="alkB"
                     /locus_tag="VN968_02290"
     CDS             complement(1421..2074)
                     /gene="alkB"
                     /locus_tag="VN968_02290"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HXL28110.1"
                     /translation="MTADLFAGVPDVRPSQELMAERAVLLRGFARPFETDLIAALRDI
                     AEQAPFRHMLTPGGHQMSVAMTNCGSVGWVTDRTGYRYDGVDPCSAKPWPAMPPSFRE
                     LAAQAADRAGFEGFSPDACLINRYQPGARMSLHQDKDETDFGAPIVSVSLGLPAIFLF
                     GGLQRSDKPRRFRLEHGDIAVWGGPARLKFHGVAPLADGEHALLGRQRINLTFRKAR"
     gene            complement(2086..2832)
                     /locus_tag="VN968_02295"
     CDS             complement(2086..2832)
                     /locus_tag="VN968_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HXL28111.1"
                     /translation="MRSHANSIRPAPPSDIAARVDAIDWTRASNDLDAQGCAVLKGLL
                     AAEECRALAALYPDDSQFRSRVVMGRHGFGRGEYKYFSYPLPDLIAEMRPALYAQLHG
                     VANRWNEAMGIDIRYPDQHAAFLKRCHAAGQTRPTPLLLQYGAGDYNCLHQDLYGEHV
                     FPLQVAILLSEPGKDFTGGEFVLTEQRPRMQSRPEVVPLRQGDAVAFAVHHRPVQGTR
                     GFYRVNLRHGVSQIRSGHRHTVGVIFHDAK"
     gene            complement(3065..3751)
                     /locus_tag="VN968_02300"
     CDS             complement(3065..3751)
                     /locus_tag="VN968_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HXL28112.1"
                     /translation="MFDLAFTIDARGTTTPLTLPIGQAVIAGWTGRDPVARDKHIAEL
                     EAIGIARPASTPIYYRVAARRLTTADRIEVSGAESSGEVEFVLIGWQGRIFVGLGSDH
                     TDRKVETYSVTVSKQMCDKPIAPVLWELEEVADHWDRMILRSFAWIDGARVLYQEGTL
                     DSMLPVAELIQRGFGDAGLPDGCAMFGGTFAAKGGIRAANRFEYELEDPVLKRSIRHG
                     YDVIELPILG"
     gene            complement(3744..>5020)
                     /locus_tag="VN968_02305"
     CDS             complement(3744..>5020)
                     /locus_tag="VN968_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXL28113.1"
                     /translation="DECLARIADTTGEGMRAFIHVDAEAAIEAAEAMDRLREVKAAPS
                     PFAGIPVSIKDLFDINGQVTRAGSRALDDSAPAEADASAVARLRRAGFIVIGRTNMTE
                     FAYSGIGINPHFGTPKGAWQRSVGHVPGGSSSGAAVSVVDRMVHGALGTDTGGSCRIP
                     AAFNGIVGFKPTQRRVPLDGGVPLSFTLDSFGPLARTVGCCAVLDAVLADESVKPLQP
                     RSIKGMRLAVPTTVALDELEEAVARTFERALETLSRQGALIERIAVPEFLDVGVMNTK
                     GGFAAAESYAWHRYLIISHGNVYDPRVAMRILRGEAISAADYIDLLEMRKSLIARSTV
                     RLAPYDALVLPTTANTPPRIADLADDKAFTAANLLALRNCTLINMIDGCAISLPCHRE
                     GEVPVGLMLAAAGGSDRRIFELAAAMEAVIRV"
BASE COUNT          924 a         1655 c         1588 g          853 t
ORIGIN      
        1 ttttgtgaaa tgggccgacc gcatcgaata tcccgacgcg gcgccgacgc tggtcagccg
       61 cgcattccag gagatgctgt cgggccggcg cgggccggtg gcgctggaaa tgccctggga
      121 cgtattcacg cagcgcgcgg aaaccggtgt ggcaaaaccg ttcgatcttt tcccagcacc
      181 gcagcccgac accgaacgcg tcaaggccgc cgccacactg atcgccagca gcaaggcgcc
      241 gatgatcttc gtcggcagcg gcgccattca cgcccgtgat gaaatcctcg agcttgccga
      301 gctgatcgac gcgccggtgg tggcgttccg cagcggacgc ggcatcgtca gcaatgcgca
      361 cgagctaggg ctgacgatgg cggcggccta tcggctgtgg ccgagcaccg atctcatgat
      421 cgggatcggc tcacgcatgg aattgccgac catgttccgc tggccgtttc gtcccgacgg
      481 cctgaagtcc gttcgcatcg atatcgatcc atcggaaatg cgccgattca ctccggacgc
      541 gccggtgatt gccgacgcgc aggccggcac acgcgaactg ctcgcagcgg tgcgaaaggc
      601 tggctacacg aaaaccagcg gacgtcgttc cgcgatccgc gaggcctcgg cggcagcgtt
      661 gcgggagatc cagaaggttc agccgcagat ggcttacctg aatatcctgc gcgaggtgct
      721 gccgcataac gccatcgtca ccgacgagct gtcgcaggtc ggcttcacct cctggtacgg
      781 ctttccggtc tacgagccgc gcactttcat tacctcaggc tatcagggca cgctcggctc
      841 gggctttccc accgcacttg gcgccaaggt ggcgcatccg gaccggccgg ttgtcgcgat
      901 aaccggcgat ggcggcttca tgttcggcgt gcaggaattg gcgaccgcag tgcagttcaa
      961 catcggcgtg gtgacgctgg tgttcaacaa caacgcctat ggcaacgtcc ggcgcgacca
     1021 gcgcgagcgt ttcgacggcc gggtggtggc gtccgacctg gtcaatccgg attttgtgaa
     1081 acttgcggaa tcctttggcg ttgctgctgc gcgcgtcacc tcacccgaga catttcgcgc
     1141 ggcgctggaa aaagcgctgg ctcacggcgg gccctacctc atcgacatcg aagtgcccaa
     1201 ggactccgag accagtccgt gggccttcat tcatccggcg aaaccgtagt tgccctcatg
     1261 gtgaggaggc gctcttgcgc cgtctcgaac catgaggcct gcacaactca tcgttcgaga
     1321 cgcggcgaag acgccgctcc tcaggatggg gcgttccgtt taaacccgcc tcacctgtat
     1381 gcgttgctga gcccgcgatg cggcgatcat cagcccagcc tcagcgcgcc ttgcggaatg
     1441 tcagattgat gcgctgccgg cccagcaagg catgctcgcc gtccgcgagc ggtgcgacgc
     1501 cgtgaaactt gagccgcgcc ggcccgcccc acaccgcgat gtcgccatgc tccagccgaa
     1561 accggcgcgg tttgtcgctg cgctgcaatc cgccgaacag gaagatcgcg ggcaaaccca
     1621 gcgataccga gacgatcggc gcgccgaaat cggtctcgtc cttgtcctga tgcaaggaca
     1681 tccgtgcacc gggctggtag cgattgatca ggcatgcatc cggcgagaag ccctcgaagc
     1741 ctgcccgatc cgccgcttgc gcggcaagtt cgcggaacga tggcggcatc gccggccagg
     1801 gtttcgccga acagggatcg acaccgtcgt agcgatagcc ggtgcgatcg gtgacccagc
     1861 caacgctgcc gcagtttgtc atcgccaccg acatctgatg gccgcccggc gtcagcatgt
     1921 ggcggaacgg cgcctgctcc gcgatatcgc gcaatgccgc gatcaggtcg gtttcgaacg
     1981 gcctggcaaa accgcgcagc agcacggcgc gctccgccat cagctcctgc gacggacgca
     2041 catccggaac gccagcaaat aaatcggccg tcaaatcaac agcactcact tcgcgtcatg
     2101 aaagatcaca ccaacggtat ggcgatggcc ggagcggatc tggctcacgc catggcgcag
     2161 attaacgcga tagaatcccc gcgtcccctg caccggcctg tggtgcacag caaaagccac
     2221 cgcatcgccc tgacggagcg gcaccacctc gggccgtgac tgcatgcgag gccgctgctc
     2281 ggtcagcacg aattcgccgc cggtgaagtc cttgcccggt tccgacagca ggatcgcgac
     2341 ctgcaacggg aacacgtgtt cgccatagag atcctgatgc aggcaattgt aatcgccggc
     2401 accgtattgc agcagcaacg gcgtcggtcg ggtctggccg gccgcatggc agcgcttcag
     2461 gaatgcggca tgctgatccg gatagcggat atcgatgccc atggcttcgt tccagcgatt
     2521 ggccacgccg tggagttgcg catacagcgc cggccgcatt tccgcgatca ggtccggaag
     2581 cgggtaggaa aaatatttgt actcgccgcg cccgaacccg tggcgtccca tcaccacccg
     2641 gctgcggaac tggctgtcgt caggataaag ggccgcaagc gcgcggcact cttcggcggc
     2701 gagcaaaccc ttcaggaccg cgcagccctg cgcatcgaga tcgttgcttg ctcgcgtcca
     2761 atcgatggca tcaacgcgtg cggcgatgtc agagggaggc gcgggacgga tactgtttgc
     2821 gtgtgatctc atggcgccga atgtcgcaaa tcctcccgtc ctgcgccacc cgatttccgg
     2881 aaggaacgga actcagcgtc ggttatctca gccagacggc agcaggtcgt catgccgggt
     2941 ttcgacccgg tcatccacgt ctcatgtagt ccgccgatct gacgacgcgg atgccccggc
     3001 atcgcggtca agtttacgcg gtctgcacaa ggcagagtgg ccgggcatga cgatccaata
     3061 taactcagcc gaggatcgga agttcgatca cgtcgtagcc gtggcggatg ctgcgcttga
     3121 gaacggggtc ttcgagttca tattcgaagc gattagcggc gcggatgccg cctttggcgg
     3181 caaaggttcc gccgaacatg gcgcaaccgt ccggcagtcc cgcatcccca aagccgcgct
     3241 gaatcagttc cgccaccggg agcatgctgt cgagtgtgcc ttcctggtac agcacgcgcg
     3301 cgccatcgat ccatgcgaac gagcgcagga tcatccggtc ccaatgatcg gcaacttcct
     3361 caagttccca gagcactggc gcgatcggct tgtcgcacat ctgcttggag accgtgacgc
     3421 tgtaggtttc caccttgcgg tcggtgtgat cggagccgag accaacgaaa atgcgaccct
     3481 gccagccgat cagcacgaac tcgacctcgc cgctcgactc cgcgccgctg acctcgatgc
     3541 ggtcggcggt ggtcagccgc cgcgcggcga cgcgataata gatcggagtc gaggcaggcc
     3601 gggcaatgcc gatcgcctca agctcggcga tatgcttgtc gcgcgcgacc ggatcgcgtc
     3661 cggtccatcc ggcgatcacg gcctgaccga tcggcagcgt cagcggggtc gtcgtacccc
     3721 gggcgtcgat ggtgaacgca agatcaaaca cgaatgacag cctccattgc ggccgcgagc
     3781 tcgaagatac ggcggtccga gccgccggcg gccgcgagca tcagcccgac cggcacttcg
     3841 ccctcgcgat ggcagggcag cgagatcgcg cagccgtcga tcatgttgat cagcgtgcaa
     3901 ttgcgcagcg ccagcagatt agccgcggtg aaggccttgt cgtcggccag atccgcgatg
     3961 cgcggcggcg tgttggcggt ggtcggcagg accagcgcgt cgtagggcgc aagacgaacc
     4021 gtcgagcgcg cgatcagcga ctttcgcatc tcgagcaagt cgatataatc agcagcgctg
     4081 atggcttcgc cgcgaaggat gcgcatcgcc acgcggggat cgtagacgtt cccatggctg
     4141 atgatcagat agcgatgcca ggcatagctt tccgccgcgg cgaaaccgcc cttggtgttc
     4201 atcaccccga catcgaggaa ttccggcacg gcaatgcgct cgatcagcgc gccctgtcgc
     4261 gacagcgtct ccagcgcacg ctcgaaggtc cgcgccaccg cttcctcaag ctcgtcgagc
     4321 gcaaccgtgg tcggcaccgc gaggcgcatg cccttgatcg atcgtggctg cagtggcttt
     4381 accgattcgt cggcgagcac ggcgtcaagc accgcgcagc atccgaccgt gcgcgccagc
     4441 ggcccaaagc tgtcgagcgt gaacgacagc ggcaccccgc cgtcgagcgg cacccggcgc
     4501 tgcgtcggct tgaagccgac gatgccgttg aaggccgccg gaatccggca tgacccgccg
     4561 gtgtcggtgc cgagcgcgcc atgcaccatg cgatcgacca cggacaccgc agcgcccgag
     4621 gacgatccgc cgggcacatg cccgacactg cgctgccagg cgcctttcgg cgtgccgaaa
     4681 tgcggattga tgccgatgcc ggaataggcg aattccgtca tattggtgcg gccgatcacg
     4741 atgaagccgg cgcggcgtaa ccgcgccacc gccgatgcgt cggcttccgc cggggctgaa
     4801 tcgtcgagcg cgcgcgaacc ggcgcgggtt acctgcccgt tgatgtcgaa caggtccttg
     4861 atcgaaaccg gaatgccggc gaatggcgac ggcgcggctt tcacctcgcg cagccggtcc
     4921 atcgcttccg ccgcttcgat ggcggcttcc gcatcgacat gaataaaggc ccgcatcccc
     4981 tcgcccgtcg tgtccgcgat ccgggcgaga cattcgtcga
//
LOCUS       DATNOI010000068         6505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_685324,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000068 DATNOI010000000
VERSION     DATNOI010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685324"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VN968_02310"
     CDS             <1..1122
                     /locus_tag="VN968_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003548932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXL28114.1"
                     /translation="KRLLAYSTIENVGIIFVSLGLALAFQANGLKLPAALAFTAALFH
                     VLNHSFFKSLLFFGAGAVLTSTGERDMDKLGGLIHRMPLTSFVFLVGCVAISALPPFN
                     GFVSEWLIFQAVLQSPQLPQWALKIMVPAVGALLALAAALAAACFVKAFGVTFLGRPR
                     SEAADAAEEVDKYSLSAMFILAALCLLAGILPGLVIDALSPITTEILGGRMPIQANEP
                     WLSIVPIAESRSSYNGLLVLVFITISASLAVYFIHRFASHALLRGPAWGCGFSDPTPA
                     AQYSGASFAQPIRRVFGTLVFRARDHVEMPAPGDIRPARLSIELHDLVWEGLYAPLAD
                     AVGFSADRLNRLQFLTIRRYLSLVFATLVTLLLVLAIWS"
     gene            1113..2069
                     /locus_tag="VN968_02315"
     CDS             1113..2069
                     /locus_tag="VN968_02315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HXL28115.1"
                     /translation="MVVISDILVQGVQMLLVLVLAPLLTGFVRKIKARLVLRQGASVF
                     QPYRDLLRLLRKEVVLADNASWLFRVTPYITFAAIWVAAALVPTFATGLLFNWTADLI
                     AIVALLGSARFFLALAGMDVGTSFGGIGSSREVMIAALAEPAMLLFVFCMALVAGSTQ
                     LSTVANFMASSYVGLRVSLGMAMIALIMVALAENGRIPVDNPATHLELTMVHEAMVLE
                     YSGRHLAMIEFGAFLKLLLYISLIACVFLPWKIAIFGTGPLSYAVGAGAYLAKLAAAG
                     FFLALFETATAKMRVFRIPQFLGAALMLGLLGTLLLFVSRSF"
     gene            2075..2737
                     /locus_tag="VN968_02320"
     CDS             2075..2737
                     /locus_tag="VN968_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-4 component E"
                     /protein_id="HXL28116.1"
                     /translation="MHSLSFDISHTLAGGLVLISFMMLYQDRLYSLLNVFALHALVLA
                     LSVAWQAYIQNAPHLYVTAAIALVFKAIVIPVALHRIVKQLGIHRDIESAVGIGPTML
                     AGMGLVALSMVLMLRVTAEADPLAREDLAFALSVVLLGLLVMVTRRNAVSQVVGFMSL
                     ENGLVLAATGAKGMPLVVEISVAFSILIAFIVIGIFLFRIRERFDSVDVSALDDYRGE
                     RK"
     gene            2734..4185
                     /locus_tag="VN968_02325"
     CDS             2734..4185
                     /locus_tag="VN968_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HXL28117.1"
                     /translation="MSTASFDAVTAVLLIPIGSAALLAILPGYRLTARLNVVASLATF
                     LSALSLLVVERPQPMPYVLVDDLNIVFIVLNTFVGFTTSIFSASYIAHELDTGRLTPV
                     YLRFYHAMYQTMMFGMNLAFVSNNIGLMWVAVEIATLTTVLMVGIYRTHAALEAAWKY
                     FILGSVGIAFALFGTILVYMAARPVVGEGQDAMVWTLLIQHAGDFDPALLNVAFVFLF
                     LGYGTKVGLAPLHAWLPDAHAEGPTPISAVLSGLLLNVALYALLRFKILLAANPAAIA
                     PGPLMVTMGLVSLVFAAFMLYRRRDIKRLFAYSSIEHMGIIVFAFGMGGPLANFAGLL
                     HMVMHSLTKSAIFYAVGHISQIKGTQRIGRIRGLTVTHPVLGWGLVMGVVAIAGLPPL
                     GIFMSEFLVVSSTFARQPLLAIVLVLGLLLAFGALMLRLTSVAFGNPRGSTKAAEASY
                     LPMFAHLSLVLVAGIYLPAPLVVWFQHVAQLLG"
     gene            4204..5715
                     /locus_tag="VN968_02330"
     CDS             4204..5715
                     /locus_tag="VN968_02330"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXL28118.1"
                     /translation="MPTLIDLMLEGCKVPQHRPWPRATVDASIWKFAAGEVARPRWSL
                     LDLWGEHATVHMAIMDEHTAEIATVSLDCPDLTFPSVGLRHPPALRLERAINDLFGLS
                     AEGLPDARPWLDHDCWGVGFPLGNRVYGLSKTSPYRFLPAEGDGLHQIAVGPVHAGII
                     EPGHFRFTASGETVVRLEQRLGYTHKGIEGLMIGADLERAVALAGRVSGDSTVAYAYA
                     FSRAAEAALRLVVPDRAVWLRALVAELERLANHLGDIGAICNDASFTLMHAHCSVLRE
                     SVLRASDGAFGHRLLRDVIAPGGVTHDLSEAGTRLIQAMLVNIRLRFPALVELYDNTA
                     SLQDRTVDTGVLTPALTKQYAAGGYVGRASGRSFDARRTLAYPPYDSLRFEVPVLNEG
                     DVNARIWIRVREVEQSLSLIDQILERLPQGPLRAQADQLREPGEGMAIVEGFRGDVLA
                     WLRVRDGRIERCRLRDPSWFQWPLLEACIENNIVADFPLCNKSFNCSYSGQDL"
     gene            5765..6295
                     /locus_tag="VN968_02335"
     CDS             5765..6295
                     /locus_tag="VN968_02335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HXL28119.1"
                     /translation="MRKLLFQSLFRQPFTEQAPPPDDAGLSELATAVGNAARRRLGRS
                     LSIREVDAGSCNGCELEIHALNNAYYDVERFGLRFVASPRHADVLMVTGPVTKNMREA
                     LERTYHAVPDPKWVVAIGDCGRDGGCFAGSYAVVGGVAEVIPVDLHILGCPPTPTAML
                     QGLLALLERADSSAVI"
     gene            complement(6299..>6505)
                     /locus_tag="VN968_02340"
     CDS             complement(6299..>6505)
                     /locus_tag="VN968_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXL28120.1"
                     /translation="ASDGVVRGNVDSLMKLVSALENAGIELINPGVASVAGGRGVRLR
                     EHVPKPKLKTVRPPKPSRAMERAR"
BASE COUNT          996 a         2087 c         2001 g         1421 t
ORIGIN      
        1 aagcgcctgc tcgcctacag cacgattgaa aacgtcggca tcatcttcgt cagcctcggc
       61 cttgcactgg cgttccaggc caacggcctg aagcttccgg cggcactcgc tttcaccgcg
      121 gcgctgtttc acgtgctcaa ccattcgttc ttcaagagtt tgctgttttt cggagccggc
      181 gccgtcctga cctcgaccgg cgagcgggac atggacaaat tgggtggcct catccaccgg
      241 atgccgctga ccagcttcgt gtttctggtc ggctgcgtgg cgatctcggc gctgccgccg
      301 ttcaacggct tcgtttcgga atggttgatc ttccaggccg tgctgcaaag tccccaactg
      361 ccgcaatggg cgctcaagat catggtgccc gcggtcgggg cattgctggc cctggccgca
      421 gccctggcgg cggcgtgttt cgtcaaggcg ttcggcgtca cgtttctcgg acgaccgcgc
      481 agcgaggcgg cggatgcagc agaggaagtc gacaagtact cgctttcggc catgttcatc
      541 ctcgccgcac tctgcctgct ggccggaatt ctgccgggac tggtgatcga cgcgctctcg
      601 ccgatcacca ccgagattct cggcggccgc atgccgatcc aggccaacga gccctggctt
      661 tcgatcgtgc cgatagcgga gagccgcagt tcgtacaacg gattgctggt gctggtattc
      721 attacgatat ccgcttcgct ggccgtctat ttcattcatc gcttcgcctc gcatgcgttg
      781 cttcggggac cggcctgggg ctgcggcttt tccgatccga cccccgccgc ccaatattcc
      841 ggcgcgagtt tcgcgcaacc gatccgccgc gtgttcggca cactggtgtt tcgcgcccgc
      901 gatcatgtcg agatgcccgc ccccggcgat atcaggccgg cgcgactgag catcgaactg
      961 catgatctgg tctgggaggg actctatgcg ccgctggcag atgcggtcgg cttctcggcc
     1021 gaccggctca atcgtctgca gttcctgacc atccggcgct atctcagtct ggttttcgcg
     1081 accctcgtca cgctgctgct ggtgctcgcg atatggtcgt gatctcggac attctggtgc
     1141 agggcgtgca gatgctgctg gtgctggtgc tggccccact gctgaccggc ttcgtgcgca
     1201 agatcaaggc ccggctggtg ctccgccagg gcgcgtcggt ttttcaaccg tatcgcgatc
     1261 tgttgcggct gctccgcaag gaggtggtgt tggccgacaa cgcgtcatgg ctgttccggg
     1321 tcacgcctta catcaccttt gcggcgatct gggttgccgc agccctggtg ccgactttcg
     1381 caaccggcct cttgttcaac tggaccgcgg atctgatcgc catcgtcgca ctactgggaa
     1441 gtgcgcgctt ctttctggcg ctggcgggca tggatgtcgg caccagtttc ggcggtatcg
     1501 gctccagccg cgaagtcatg atcgcggcgc tggcggagcc ggcgatgctg ctgtttgtct
     1561 tttgcatggc gctggtggcc ggttcgaccc aattgtcgac ggtcgccaat ttcatggcct
     1621 cctcctacgt cggattgcgg gtatcgctgg gaatggcgat gatcgccctg atcatggtgg
     1681 cgctcgcgga gaacgggcga atcccggtcg acaatccggc aacgcatctc gagctcacca
     1741 tggtgcatga ggcgatggtt cttgaatatt ccggacgcca tctggcgatg atcgaattcg
     1801 gggcctttct caagctgctg ctctacatct ccctgattgc ctgcgtgttc ctgccctgga
     1861 agatcgcgat cttcggcacc ggaccattgt catatgccgt cggcgccggc gcctatcttg
     1921 ccaagcttgc ggcggcgggc tttttcctcg cgctgttcga gaccgccacg gcgaaaatga
     1981 gagttttccg cattccacaa tttctcggtg ccgcgctcat gctgggcctg ctcggtacgc
     2041 tcctgctgtt cgtgtcgagg agtttctgat gcaaatgcac agcctctcct tcgacatctc
     2101 gcacacgctc gccggcggac tggtcctgat cagcttcatg atgctgtacc aggaccggct
     2161 ttattcgctg ctcaacgtct ttgctctcca tgctttggtg ctcgcgcttt ccgtcgcctg
     2221 gcaggcctat atccagaacg cgccgcatct ttacgtcacc gcggccatcg ccctggtgtt
     2281 caaggcgatc gtcattccgg tggcgctgca ccgcatcgtc aagcagcttg ggattcaccg
     2341 cgacatcgaa tcggccgtgg gtatcggccc caccatgctg gccgggatgg ggctggtcgc
     2401 cctctcgatg gtcctgatgc tgcgggtgac cgccgaggcc gacccgctgg cgcgcgagga
     2461 cctcgccttc gccttgtcgg tggtgctgct cggactgctg gttatggtga cccgccgcaa
     2521 cgccgtcagt caggtggtgg gattcatgtc gctcgagaac ggactggtgc tggcggccac
     2581 cggcgccaag ggcatgccgc tggtcgtcga gatcagcgtg gcgttctcga tcctgatcgc
     2641 cttcatcgtc atcggcatct tcctgttccg gatccgtgaa cgcttcgact ccgtcgacgt
     2701 gtccgcgctc gacgactacc ggggcgagcg gaaatgagca cggcttcctt cgatgcagtc
     2761 accgcggttc tgctgattcc gatcggatcg gcggcgttgc tggccatcct tcccggctac
     2821 cggctgacgg cgcgcttgaa cgtggtcgct agcctcgcga catttctatc ggcgctttcg
     2881 ctgctggtgg tcgagcgtcc gcagccgatg ccatatgtgc tggtcgacga tctgaacatc
     2941 gtcttcatcg tgctcaatac cttcgtgggc ttcaccacca gcatcttcag cgcgagctac
     3001 atcgcccacg aactcgatac cggccggctg acgccggtgt atttgcgttt ctaccacgcc
     3061 atgtatcaga ccatgatgtt cggcatgaac ctcgccttcg tgtcgaacaa tatcggcctg
     3121 atgtgggtcg ccgtcgaaat cgcaaccctg accacggtgc tgatggtcgg catctatcgc
     3181 acccacgccg cgctggaggc ggcgtggaag tactttatcc tcggcagcgt cgggatcgcg
     3241 ttcgcgctgt tcggcaccat cctggtctac atggcggcgc ggccggtcgt cggcgagggc
     3301 caggacgcca tggtctggac gctcttgatc cagcatgccg gggatttcga tccggcgctg
     3361 ctcaatgtcg ccttcgtatt cctgtttctc ggctatggga ccaaggtcgg cctcgcgccg
     3421 ctgcatgcct ggctgcccga tgcgcacgcc gaaggtccaa ccccgatatc ggctgtgttg
     3481 tcgggcctgc tgctgaacgt cgcgctctat gcgctattgc gcttcaagat cctgctggcg
     3541 gccaatccgg cggcgattgc ccccggcccg ctgatggtga cgatgggcct ggtttcgctg
     3601 gtgttcgcgg cgttcatgct ctaccgccgg cgcgacatca aacggctgtt cgcctattcc
     3661 tcgatcgaac acatgggcat catcgtgttt gcgttcggaa tgggcggccc gctcgccaat
     3721 tttgccgggc tcttgcacat ggtgatgcac agtctgacca agtcggcgat tttctacgcc
     3781 gtcggccata tttcccagat caagggcacg caacggatcg gcaggatccg cggcctgacg
     3841 gtcacccatc cggtgcttgg ctggggcctg gtgatgggcg tcgtcgcgat cgccggactg
     3901 ccgccgcttg gcattttcat gagcgaattt ctcgtcgtca gttcgacctt tgcgcggcag
     3961 ccgctgctcg cgatcgtgct ggtgctcggg ctgttgctgg cgttcggcgc cttgatgctg
     4021 cgcctgacca gcgtcgcctt cggaaacccg cgcggcagca cgaaagcggc cgaggcgtcg
     4081 tatctcccga tgtttgcgca tctctcactg gtcctggtcg cgggaatcta tcttccggcg
     4141 ccgctcgtgg tctggttcca gcatgtggct caactccttg gatagttttt aggattaacc
     4201 gatatgccaa cgctgatcga tctcatgctg gagggctgca aggtcccgca acatcgccca
     4261 tggccgcggg caacggtcga tgcttccatc tggaaatttg ccgcaggcga agtggcacga
     4321 ccgcgttgga gcctgctcga tctctgggga gaacacgcca ccgtgcacat ggcgatcatg
     4381 gatgaacaca cggcggaaat cgccacggtc agcctggatt gtcccgatct cacttttccc
     4441 tcggtcggcc tgcgccaccc gccggcgctt cgactggagc gcgccatcaa cgatctgttc
     4501 gggttatcgg cggaaggctt gcccgatgcc cgtccctggt tggatcacga ttgttggggc
     4561 gtgggcttcc cgttgggaaa tcgcgtctac gggttgtcga aaacatcgcc ctatcgcttc
     4621 ctgccggcgg aaggtgacgg cctgcatcag atcgcggtcg gtccggtgca cgcggggatt
     4681 atcgagcccg gacatttccg ctttaccgcc agcggcgaga ccgtggtccg gctggagcag
     4741 cggctcggat acacccacaa gggcatcgag gggctgatga tcggagccga tctcgagcgg
     4801 gccgttgcac ttgccgggcg cgtatcgggt gacagcacgg tcgcctatgc ctatgcgttt
     4861 tcgcgggccg ctgaggccgc cttgcggctc gtcgtgcccg atcgcgcggt ttggctgcgg
     4921 gcgctggtcg ccgaactcga gcggcttgcc aaccatctcg gcgacatcgg cgcaatctgc
     4981 aacgatgcct ccttcaccct gatgcacgcg cattgcagcg tattgcggga gagcgtgctg
     5041 cgcgcatccg atggcgcctt cggtcaccga ttgttgcggg atgtcatcgc acccggcggc
     5101 gtgacccacg acctcagcga agcagggaca agactaatcc aggcgatgct ggtcaatatc
     5161 cgcctgcgct ttccggccct ggtcgaactc tacgacaaca ccgcgtcgct gcaggaccgc
     5221 accgtcgata ccggcgtgct cacacccgca ttgacaaaac aatatgccgc gggaggctat
     5281 gtcggccgcg cctccggccg gtccttcgat gcgcgccgga ccctggccta tccgccctat
     5341 gacagcctgc gcttcgaggt cccggtgctg aacgagggcg atgtcaatgc ccggatctgg
     5401 attcgcgtcc gcgaggtcga gcagagtctt tcgctgatcg accagatact ggagcggctg
     5461 ccgcaaggcc ccctgcgagc gcaagccgac caactgcgag aacccgggga aggcatggcc
     5521 atcgtcgagg gatttcgcgg cgatgttctg gcctggctgc gcgtccgcga tggccggatc
     5581 gagcgatgcc gcctgcgcga ccccagctgg tttcaatggc cgctgcttga ggcttgcatc
     5641 gaaaacaaca tcgttgcgga ttttccgctc tgcaacaagt cgttcaactg ctcctactcg
     5701 ggccaggatc tctgagaaca gcttcgctga aaacttaccg acagctcgtc aaggacagca
     5761 cgaaatgcga aaactgcttt ttcaaagcct gtttcgccaa ccattcaccg agcaggcgcc
     5821 gccaccggac gatgccgggt taagcgaact cgcaaccgcc gtcggcaacg cggcgcggcg
     5881 ccggctcggc cgcagtcttt ccatccgcga ggtcgatgcc ggatcctgca acggatgcga
     5941 gctcgagatt cacgcgctca acaacgccta ctacgatgtc gaacgcttcg gcctgcggtt
     6001 cgtcgcctcg ccgcgccatg ccgatgtgtt gatggtgacc ggtcccgtga ccaagaacat
     6061 gcgcgaagcg ctggagcgca cctatcatgc ggtgcccgat cccaaatggg tggtcgcgat
     6121 cggagattgc ggtcgcgacg gcgggtgctt tgccggcagc tacgccgttg tcggcggcgt
     6181 tgccgaggtg atcccggtcg atctccacat tctcggctgc ccccctacgc cgacggcgat
     6241 gctgcaaggt ctcttggccc tgctcgaacg ggcggacagc agcgcggtta tctgatcttt
     6301 atcgcgcgcg ctccatggcc cgcgacggct tcggcggccg gaccgtcttc agctttggtt
     6361 ttggaacatg ctcgcggagt cgcacgccgc ggcctccagc cacactcgcc acgcccggat
     6421 tgatcaactc gatgcctgca ttctcaagcg cggaaaccag tttcatgagg gaatcgacgt
     6481 tgccgcggac cacgccatca ctggc
//
LOCUS       DATNOI010000069         3011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_683027,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000069 DATNOI010000000
VERSION     DATNOI010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683027"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /gene="tcuB"
                     /locus_tag="VN968_02345"
     CDS             <1..991
                     /gene="tcuB"
                     /locus_tag="VN968_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HXL28121.1"
                     /translation="DLNYLANLCHGCGACYTDCQFSPPHEFNVNVPKTLAVARAESYA
                     AYAWPRAFSGLFARNGLAISIIAAISVAAFIFGFAAFNDRQVLFGVHTGPGAFYKLMP
                     HNAMALLFGAVFVYAIAALVMGVRAFWRDIGEPIGMRVEPKSLWQAMKDAAQLRYLDG
                     GGVGCVNEDERPSDLRKTYHHFTFYGFMLCFASTMVATLYHYLIAREAPYPWWDLPVA
                     LGTLGGIGLLIGPAGLLKEKWKRDPALVDEQRTGMDVAFVAMLFLTSLTGMALLLLRD
                     SAAMGPLLALHLGVVFSLFITMPYGKFVHGIYRYVALVRYARERQQAGHAPTV"
     gene            complement(1099..1512)
                     /locus_tag="VN968_02350"
     CDS             complement(1099..1512)
                     /locus_tag="VN968_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HXL28122.1"
                     /translation="MTRHVIAAVDELPPGTRKFLTIDERPIAVFNIKGEFFGLLNRCP
                     HQGAALCEGPLIGLAQSSDPGEIEYTRLGEIIRCPWHGWEFDIRTGQSYCDPKRFRAK
                     AYPVNVEPGASVVKGPYVAETLAVSVESDYVVVDL"
     gene            complement(1570..2679)
                     /locus_tag="VN968_02355"
     CDS             complement(1570..2679)
                     /locus_tag="VN968_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL28123.1"
                     /translation="MNVQFRAGAEPASSVAVKSAIADCDIHPARATATELYPYLAKRW
                     HDHLETYGKHPYQGMMEGPPYPKAQPNASRRDAWPPEGGPQGSSLSFMQKQHLDPNNV
                     ALGVLCPLNTGQGLRNHDLSAALASAINDWQIDKWTSQDSRLKGSVVVANEDGPAAAA
                     EIRKRAGDKNFVQVLLLSRNVEPLGQRRYWPIYQAAEEAGLPIGVHAFGFGGNPITAS
                     GWPSYYIEEMVGHSQCQQTALASIVLEGVFERHPKLKMVMIEAGFGWAPSLSWRLDKN
                     WQRMKSEVPHVKRPPSEYIRDHIFWTTQPMEDPERRDHLFEVIDWVGWDKLLFATDYP
                     HWDYDDPSRVLPAGVSDANREAFYLDNARKLYGLA"
     gene            complement(2676..>3011)
                     /locus_tag="VN968_02360"
     CDS             complement(2676..>3011)
                     /locus_tag="VN968_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL28124.1"
                     /translation="YLWRLHKFWRGVRMETPWVDRAPMEIVRSNIRFSLQPIDAPPDP
                     ATLNRLFDHMQSDELLLFSTDYPHWQFDDDAVLPEGMSSDLVRKIMIDNPHATYSRLK
                     SAVTQETTP"
BASE COUNT          518 a          938 c          932 g          623 t
ORIGIN      
        1 ggacctgaat tatctcgcca atctctgcca cggctgcggc gcctgctata ccgactgcca
       61 gttctcgccg ccgcatgaat tcaacgtcaa tgtgccgaag acgcttgccg tcgcgcgcgc
      121 cgaatcctat gccgcctatg cttggccgcg cgccttctcc ggcctgttcg cgcgcaacgg
      181 cctcgccatc agcatcattg cggccataag cgttgcggcg ttcattttcg gctttgccgc
      241 cttcaatgac cggcaggtcc tgttcggagt ccataccggg ccgggcgcgt tctacaagtt
      301 gatgccgcat aacgcgatgg cgctgctgtt cggtgcggtg tttgtttatg cgatcgcagc
      361 gctggtcatg ggcgtgcgcg cgttctggcg cgatatcggc gagccgatcg ggatgcgcgt
      421 cgagcccaag tcgctctggc aggcgatgaa ggacgcggcc cagctgcgct atctcgatgg
      481 cggcggcgtc ggctgcgtca acgaagacga gcggccaagc gacctgcgca agacctatca
      541 ccattttacg ttctacggct tcatgttgtg tttcgcgtcc accatggtgg cgacgctgta
      601 tcattacctg atcgcgcgcg aggcgccgta tccatggtgg gacctgccgg tggcgctggg
      661 cacgctgggc gggatcggcc ttctgatcgg gcctgccggc ctgcttaagg aaaaatggaa
      721 acgcgatccg gcgctggtcg acgagcagcg caccggcatg gatgtcgcgt tcgtcgccat
      781 gctgttcttg accagtctga ccggcatggc gctgttgctg ttgcgggaca gcgctgcgat
      841 ggggccgctg ctggcgctgc atctcggcgt ggtgttttcc ctgttcatca ccatgcccta
      901 cggcaaattc gtgcacggca tctaccgtta tgttgcgctg gtacgttacg cgcgggagcg
      961 gcagcaagcg gggcatgcgc cgacagttta gccttcctca ccctgaggag cggccacttt
     1021 gggccgcgtc tcgaagggtg aactgcgcga ccgtccttcg agacgcgcgc aagagcgcgc
     1081 tcctcaggac gaggacttct acaaatccac caccacgtaa tcgctttcca ccgacaccgc
     1141 gagcgtctcc gccacgtagg gtcccttcac cacgcttgcg ccgggttcga cgttgaccgg
     1201 ataggctttg gcgcgaaaac gcttggggtc gcagtaggat tggccggtgc ggatatcgaa
     1261 ttcccagccg tgccagggac agcggatgat ttcgccgagc ctcgtatatt cgatctcgcc
     1321 gggatcggac gactgcgcga gcccgatcag cggcccttcg cacaacgcag caccctgatg
     1381 cgggcagcgg ttcaacagac cgaagaactc gcccttgata ttgaacaccg cgatcgggcg
     1441 ctcatcgatg gtgaggaatt ttcgcgtgcc cggcggcaat tcatccaccg cggcgatgac
     1501 atgccgagtc atgttatttt ccgacgcatg atcttttcgg aaaaccggtt cccacttttc
     1561 cggatcatgc tatgcaagcc cgtacagctt ccgcgcatta tcgagataaa acgcttcgcg
     1621 gttggcgtcg ctgacaccgg ccgggagcac ccgcgacgga tcgtcgtaat cccaatgcgg
     1681 atagtcggtc gcgaataata gcttgtccca gccgacccag tcgatcacct cgaacagatg
     1741 atcgcgcctt tcggggtctt ccatcggctg cgtggtccag aagatgtggt cgcggatgta
     1801 ttccgacggc ggacgcttga catgcggcac ctcgctcttc atccgctgcc agttcttgtc
     1861 gagccgccac gacagcgacg gcgcccagcc gaagccggct tcgatcatga ccatcttcag
     1921 tttcgggtgc cgctcgaaca cgccttccag cacaatgctc gcaagcgcgg tctgctggca
     1981 ctgcgaatgg ccgaccatct cctcgatgta atagctcggc cagcctgaag ctgtgatcgg
     2041 attgccgccg aagccaaaag catgcacgcc gatcggaagt cccgcctcct cggcggcttg
     2101 atagatcggc cagtagcggc gttgacccaa aggctcgacg ttgcggctca acagcagtac
     2161 ctggacaaag ttcttgtcgc cggcgcgctt gcggatttcg gcggccgccg ccggaccatc
     2221 ctcattggcg accacgacgg agcctttcag gcggctgtcc tggctggtcc atttgtcgat
     2281 ttgccagtcg ttgatcgcgc tcgccagtgc cgcgctcaga tcgtggttgc gaaggccctg
     2341 cccggtgttg agcggacaca atacgccgag cgcgacgttg ttcggatcga gatgctgctt
     2401 ttgcatgaac gacagcgagg agccctgcgg accgccttcc ggcggccagg cgtcgcggcg
     2461 cgacgcgttc ggctgcgcct tcggataggg cgggccttcc atcatgccct gatagggatg
     2521 cttgccgtag gtttcgagat gatcgtgcca gcgtttggcg agatacggat agagctcggt
     2581 cgcggtcgcg cgcgccggat ggatatcgca atcggcgatc gctgatttga cggcgacgga
     2641 tgaagcgggc tcagcgccgg ctcggaactg cacattcatg gcgttgtctc ctgcgtcacc
     2701 gcgctcttca ggcggctgta ggtcgcgtgc ggattgtcga tcatgatctt gcgcacaaga
     2761 tccgaagaca tcccctcggg cagcaccgcg tcatcgtcga attgccagtg cggatagtcg
     2821 gtcgagaata ggagcaattc atccgactgc atatggtcaa acaggcggtt cagggttgcc
     2881 ggatccggcg gcgcgtcgat cggctgcagc gagaaccgga tgttgctgcg cacaatttcc
     2941 atcggcgcgc ggtccaccca cggcgtttcc atccgcacgc cgcgccagaa cttgtgcagc
     3001 cgccacagat a
//
LOCUS       DATNOI010000070        10455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1191366,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000070 DATNOI010000000
VERSION     DATNOI010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191366"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VN968_02365"
     CDS             <1..886
                     /locus_tag="VN968_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HXL28125.1"
                     /translation="DTITGSAGNDLVNGGRGNDVASLGAGNDTFVWNPGDGSDVVEGQ
                     GGTDTLQFNGAPADEKIDISANGGRAILFRDVGNVTMDLNDVERINLVALGGVDTITV
                     DDLTRTDVNQVAIDLGATPGNSQGDGQVDTVVINATNGSDTIIVTNNNGVVTVTGLAT
                     DVTISGFDADDKIVINGLGGDDVIEASGLRGMQLTANGGDGNDILIGSRGNDVLNGGA
                     GDDVLIGNGGLDTLDGGTGNNTVINAPAVASPAASAALLGQFMASSFVSAGEGHGALP
                     FADPSAGQQPQLALPHAA"
     gene            912..3056
                     /locus_tag="VN968_02370"
     CDS             912..3056
                     /locus_tag="VN968_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HXL28126.1"
                     /translation="MSTETNAARPTDPGLEALVTLLHLQGVAADAGQIAHRLGTDKIG
                     APEMLRCAKDLGLKARAYRTDWSRLGSTPLPAIASLNDGGFLLVAKADGNKVLVQSPL
                     ASRPALMTREDFAAVWDGRLILMTRRAGLSDISRRFDITWFLGAIHKYRHLLSEVLVA
                     SFFLQLFGLVSPLFFQVVIDKVLVHQSLGTLDVLVLGLVTISVFETVLGILRTYLFAH
                     TTNRIDVELGARLFRHLLALPMAYFQARRVGDSVARVRELENIRNFLTSSALTLLIDL
                     FFTFVFLGVMFFYAPLLTWIVLGSFPFYIAISAGATPLFRRRLDEKFRRGAENQAFLV
                     ESVTGIETLKAMAVEPQMQRRWEEQLAGYVAASFRVLSLGNSASQAVQLVSKIAMAGL
                     LYYGAQLVIDGSLSVGELVAFNILAGRVSAPVLRLAQIWQDFHQARLSIARLGDILNT
                     TAEPTYQAGRARLPAIRGDIKFDHVSFRYRIDGQEVLHDVSFEVPAGQTVGIVGPSGS
                     GKSTFAKLVQRLYVPERGRVLVDGMDLSMADPAWLRRQIGVVLQENVLFNRSVRDNIA
                     LADPAMPMERVIAAARLAGAHEFILELTEGYDTLVGERGSTLSGGQRQRIAIARALVT
                     DPRILIFDEATSALDYESERIVQQNMKDIANGRTVLVIAHRLSTVRAADRIVTLDRGR
                     LVEDGTHDALIKTSGRYASLHRLQAGVLDEVG"
     gene            3043..4497
                     /locus_tag="VN968_02375"
     CDS             3043..4497
                     /locus_tag="VN968_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HXL28127.1"
                     /translation="MKSAEAAASAIPMQRVVPFRRPPERRGEELAFLPAALEIVETPP
                     SPTGRAIGATIILLFCVALMWAWWGTIDIVASATGKILPSGRTKVVQPFETGVVRSIR
                     VQDGQAVKAGDVLIELDPTVNAAERDHQRNDLLAEQLNIARLRAALAGGDDPVADFKP
                     SADADPELIGTQRQLLLNQVTEHRAKIAALFRQQAQKEAEHATTAATIHKLETIIPVI
                     QSRVDIRKTLVEKELGSKLSYLEVVQLLVEQQEDFNVQNSHLKETEAAIAAIRETRGQ
                     AAAEYRHTLSGELAKAEQKASGLAQDLIKAEQKTRLQLLTAPVDGVVQQLAIHTVGGV
                     VTPAQSLLVVVPSDSRLEIEAMVSNRDIGFVHSGQQAEIKVDTFNFTRYGLLRGEVLN
                     VSQDAVIRDRQPDRSGNRAPGIPGDSSEPKGQELNYSARISLDRTNMRIDDKMVNLSP
                     GMAVTVEIKTGSREILSYLLSPLLRYRQETLRER"
     gene            complement(4770..4955)
                     /locus_tag="VN968_02380"
     CDS             complement(4770..4955)
                     /locus_tag="VN968_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28128.1"
                     /translation="MCDAEDDLFFKGQPQQLYDHLTCRKTMMRFTKAEGAGAHCQVGA
                     GRLAFARIFDWLDETLA"
     gene            complement(4948..5247)
                     /locus_tag="VN968_02385"
     CDS             complement(4948..5247)
                     /locus_tag="VN968_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28129.1"
                     /translation="MNRRALLALGIPLATMAASGARAEDTPSGAAPLAPTAMDKKPLF
                     PDDVQFWYETQRAFGAAEYGGAQFGEVLATSARIKSGDYWYDERRRGHKLPDPGV"
     gene            5408..5971
                     /locus_tag="VN968_02390"
     CDS             5408..5971
                     /locus_tag="VN968_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL28130.1"
                     /translation="MTAKPRPGGRSARNKAAVFEAAAALLVERGHEAVTMTDIAERAG
                     MAATSLYRRWGDVRALMMEVAVEKLMRERPLPDTGSLRGDLLAWARPIATSLASREGS
                     SFFRAVIATTTPAGADGSLRRAALKGRSEQMELMLERARQRGEKPPGVAELLDYVLAP
                     LYMRALFGRPLSKAVADRLVDRLVARS"
     gene            6114..6563
                     /locus_tag="VN968_02395"
     CDS             6114..6563
                     /locus_tag="VN968_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL28131.1"
                     /translation="MAKLSINGQTVDIDVEPDTPLLWAIRENIGLTGTKYGCGIAQCG
                     ACTVHIDGVAVRSCGVQVSEAVAKRITTIEGLAVNGALTKVQQAWIDNDVPQCGYCQS
                     GMIMAVTALLKEKPKPTDADIDDAITNICRCGTFQQVREAIHAAANA"
     gene            6574..8778
                     /locus_tag="VN968_02400"
     CDS             6574..8778
                     /locus_tag="VN968_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL28132.1"
                     /translation="MTHMPQLNRRAFVVGAAAAGAGLALGFDVPFGGPTVVRAADGTP
                     EVNAWVVIRPDDTVVIRIARSEMGQGTLTGLAQLVAEELECDWSKVTTEYPTPGQSAA
                     RKRVWGSFSTGGSIGVRASQQDVRKGGATARMMLVQAAANEWKVPVAECTAANSVITH
                     TPSGRTISFGKVAEAAARVEPPVEVKLKDPKDWKIAGKPLKRLDTSDKTTGKMIYGID
                     VKLPGMLNAAIKDCPVFGGKLKSFDEAKITGMKGVKKVVRVGDSAVAVVADTWWRAKT
                     ALDALPVVWDEGDNAKVSSASIAKWLEEGLDPVQPAFVGNANGDAKAAIASAAKKIEA
                     VYGYPHQNHAALEPLNATALYTPDKCEVWTGTQDAELAYTVTAKVSGLPPEKCDIHKV
                     MLGGGFGRRNAPDYVRQAVLIAKQMPGTPVKLLWSREEDMTHGKYHPVTQCKLTGAFD
                     ADNNLTCLHMRISGQSVFASITPQVLKDGMDPFTFQGVAPTGEAFASIGPARLGYTIP
                     HLLIDHSMRNPHVPPGVWRGVNGNQNGFYLECFMDELAHAAGQDPLEFRRKLMADHPK
                     HLAVLNAVAERVGWSKPAPQGIFRGIAQQMGLGSYVAAAAEISVTDGNKIKVHRIVAA
                     IDSGTVVNPAQVERQVAGSFVYGLSALFYGECTVKDGRIEQTNFDTYNSMRISEMPKV
                     ETIVMPSGGFWGGVGEPTITVAAPAVLNAYFAATGKRIRSVPLKNQDIAFAW"
     gene            8870..>10455
                     /locus_tag="VN968_02405"
     CDS             8870..>10455
                     /locus_tag="VN968_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HXL28133.1"
                     /translation="MKIIDAPLLLRTAAITITIAASYSVAQGQVAPKGPEPATLKANA
                     ELAKTLPFANRQDFDDAMRGFIGTTPDALVTGTGPRPVWSMKPYDFLKQNEVADSVNP
                     SLWRQAQLNAIHGLFKVTERVYQVRGFDISNMTIIEGDTSLIIIDVLLTTETARAALE
                     LYYQHRPKKPVGTVIYSHSHGDHFGGVKGVISEADVVAGKVQVLAPAGFLEAVAGESV
                     MAGTAMSRRAQYQFGPLLPPGPRGHVDTGLGKNVARGTITLIAPTGTIDKTMEKRVID
                     GVEIVFQLAPDSEAPSEMLMYFPQFRVLDMAEDVTHNMHNLYTIRGSEVRDGNLWSRY
                     ISEAMETFGDRSDVIIAQHHWPVAGQARVVALMKKQRDMYKFINDQSLRLLNLGYTPG
                     EIAETLHMPASLEQEWSARGYYGTLRHNAKAVYQKYLGWYDANPADLDPLPPAENARK
                     TVEYMGGADAVIARARGDFAKGEYRWVASAMNQVVFADPANRAARELGADALEQLGFQ
                     AEAATWRNAYLVGAMELRNGV"
BASE COUNT         2030 a         3316 c         3307 g         1802 t
ORIGIN      
        1 tgacaccatc accggcagcg cgggtaacga cctcgtcaac ggcgggcgcg gcaatgatgt
       61 ggcttccctt ggcgccggca acgacacctt cgtctggaac ccgggcgacg gcagcgacgt
      121 ggtcgaaggc cagggcggaa ccgatacgct ccagttcaac ggcgcccccg cggacgagaa
      181 gatcgacatc tcggccaatg gcgggcgggc gatcctgttc cgcgatgtcg gcaacgtcac
      241 tatggacctc aacgacgtcg agcgcatcaa cctcgtggcc ctcggcggcg tcgacaccat
      301 cacggtcgac gatctgaccc ggaccgacgt gaaccaggtt gccatcgacc tgggtgcgac
      361 gccaggcaac agccagggcg acggccaggt cgacacggtc gtcatcaacg cgaccaacgg
      421 cagcgacacc atcatcgtca ccaataacaa cggcgtggtc acggtgacgg gtctggctac
      481 cgacgtgacg atctccggct tcgatgccga cgataaaatc gtcatcaatg gccttggcgg
      541 cgacgacgtc atcgaggcgt cgggactgag gggcatgcag ctcaccgcca atggcggcga
      601 tggcaacgac atcctgatcg gcagtcgtgg caacgacgtt ctgaacggcg gggcaggaga
      661 tgacgtgctg atcggcaatg gcgggctgga caccctcgat ggcggaactg gcaacaacac
      721 cgtcatcaac gcccctgcgg tcgcctcgcc ggccgcgagt gccgccttgc tgggccagtt
      781 catggcgtcg agcttcgtct cggcgggtga aggtcatggc gcgctgccgt ttgccgatcc
      841 gtcggcaggc cagcagccgc agctcgcgct gccgcacgcc gcctgacgcg acctcgccct
      901 ggagagcacc catgagcacc gaaaccaacg ctgcgagacc gaccgatcct ggtctcgaag
      961 cacttgtgac gctgctgcat ctccagggcg ttgcggccga tgccggacag atcgcgcatc
     1021 ggctcggaac ggacaagatt ggcgcaccgg agatgctccg gtgcgccaag gatctcgggc
     1081 tcaaggcgcg tgcctaccga accgactggt cgcggctcgg cagcacgccc ttgcccgcca
     1141 tcgcgtccct gaatgatggc gggttccttt tggtcgccaa ggccgacggg aacaaggtcc
     1201 tggtccagtc accgctggca tcccggcccg ccctgatgac gcgggaggat ttcgctgcgg
     1261 tctgggacgg ccggctgatc ctgatgaccc gccgcgccgg attgtcggat atcagccgcc
     1321 ggttcgacat cacctggttc ctcggggcga tccacaaata ccgccatctc ctgagcgagg
     1381 tgctggtggc gtcgttcttc ctgcagcttt tcggcctggt ctcgccgctg ttcttccagg
     1441 tggtcatcga caaggtgctg gtgcaccaat ccctgggcac gcttgacgtg ctggtgctcg
     1501 gactggtgac catctcggtt ttcgagaccg tactgggaat cctgcggacc tatctgttcg
     1561 cgcacaccac caaccgcatc gacgtcgaac ttggggcgcg gctgtttcgc cacctgctgg
     1621 ctttgccgat ggcctatttc caggcgcggc gcgtcggcga ctcggtggcg cgggtacgcg
     1681 aattggagaa catccgcaat ttcctcacca gctccgccct gacgcttctc atcgacctgt
     1741 tcttcacctt cgtgttcctg ggcgtgatgt tcttctacgc gccgctgctg acctggatcg
     1801 tgctcggctc gttcccgttc tacattgcga tctcggccgg ggcgacgccg ctgtttcggc
     1861 ggcggctgga tgagaaattc cggcgcggcg ccgagaacca ggccttcctg gtcgaaagcg
     1921 tgaccgggat cgagacgctc aaggccatgg cggtcgagcc gcagatgcag cgccgctggg
     1981 aagagcagct cgccggctac gtggctgcga gcttccgggt gctgagcctc ggcaacagcg
     2041 cgagccaggc ggtgcaactc gtcagcaaga tcgccatggc aggcctgctt tattacggtg
     2101 cgcagcttgt gatcgacggc agcctttcgg tcggcgagct cgttgccttc aacatcctgg
     2161 ccggccgggt gagtgcgccg gtgctgcgcc tcgcgcagat ctggcaggac tttcatcagg
     2221 cgcggctgtc gatcgcgcgg ctgggtgaca tcctcaatac caccgccgaa ccgacctacc
     2281 aggcggggcg cgcgcggctg ccggcgatcc gcggcgacat caagttcgat cacgtctcgt
     2341 tccgctatcg catcgacgga caggaagtcc tgcacgatgt ctctttcgag gtgccggccg
     2401 ggcagaccgt cggcatcgtc gggccctcgg ggtcgggcaa aagcacattc gcgaaactgg
     2461 tgcagcgcct ctacgtcccg gagcgcgggc gcgtgctggt ggacggcatg gatctttcca
     2521 tggccgatcc cgcctggctg cgccgccaga tcggcgtcgt gctgcaggag aacgtgctgt
     2581 tcaaccgctc ggtgcgcgac aatatcgcgc tcgccgatcc ggccatgccg atggaacgcg
     2641 tcatcgcggc ggcacggctt gcgggagcac acgagttcat cctcgaactc accgagggct
     2701 acgacaccct ggtcggcgag cgcggctcga ccctgtcggg cggccagcgc cagcgcatcg
     2761 ccattgcgcg cgcgctggtc accgacccgc gcatcctgat cttcgacgag gcgaccagcg
     2821 cgctggacta tgagagcgag cgcatcgttc agcagaacat gaaggatatc gccaacggaa
     2881 ggacagtgct ggtgatcgcg catcggctct cgaccgtgcg ggcggcggac cgcatcgtca
     2941 cgctcgatcg cggccgcctg gtcgaggacg gcacccatga cgcgctgatc aagaccagcg
     3001 ggcgttatgc atcgctgcat cggctgcagg cgggagtgtt agatgaagtc ggctgaagcg
     3061 gcggcgtccg ccatccccat gcaacgcgtc gtcccgtttc ggcggccgcc ggagcggcgc
     3121 ggcgaagagc tggcgtttct gccggcggcg cttgaaatcg tggaaacgcc gccgtcgccg
     3181 accggccgtg cgattggggc caccatcatc ctgctgttct gcgtggcgct gatgtgggcc
     3241 tggtggggta ccatcgacat cgttgcatcg gcgaccggaa aaatcctgcc gagcggccgc
     3301 accaaggtcg ttcagccatt cgagaccggg gtggtgcggt ccattcgggt gcaggacggg
     3361 caggcggtca aggccggcga tgtgctgatc gagctcgatc cgaccgtcaa cgcggccgaa
     3421 cgcgatcacc agcgcaacga cctcctcgcc gagcagctca acatcgcgcg gttgcgcgcg
     3481 gcgctcgccg gcggtgatga tcccgtggcc gacttcaagc catcagccga tgcagacccg
     3541 gaactgatcg gcacccagcg tcagctttta ctgaaccagg tgacggagca tcgcgccaag
     3601 attgccgcgc tctttcgtca gcaggcgcag aaggaagccg agcacgccac gaccgcggcg
     3661 acgatccaca agctcgagac catcattcca gtgatccagt cgcgcgtcga catccgcaag
     3721 acgcttgtgg agaaagagct gggctcgaag ctcagttatc tagaggtggt ccagctattg
     3781 gtcgagcaac aagaggattt caacgttcag aacagccatc tgaaggagac ggaggcggct
     3841 atcgccgcga ttcgcgagac gcgcggccag gcggcggcag aatatcggca tacactctcc
     3901 ggcgaactcg ccaaggccga gcagaaggcg agcgggctcg cccaggattt gattaaggcc
     3961 gaacagaaga ccaggcttca actgctgacg gctcccgtgg atggcgtggt gcagcagctc
     4021 gcgatccaca cggtcggcgg cgtggtcacg cctgcgcagt ccctgctcgt ggtcgtgccg
     4081 agcgacagcc ggctggaaat cgaggccatg gtgtcaaacc gcgacatcgg cttcgtccac
     4141 tccggccaac aagccgagat caaggtcgac accttcaatt tcacccgcta cggcctgctg
     4201 cgcggggagg tgcttaacgt ctcgcaggac gccgtgatcc gcgaccgcca accggaccgc
     4261 tccggcaacc gggcgccggg aatcccaggc gacagcagcg agccgaaagg ccaggaactg
     4321 aattatagcg cgcgcatttc gctcgaccgc accaacatgc ggatcgacga caaaatggtc
     4381 aatctatccc ccggcatggc ggtgacggtg gaaatcaaga ccggctcgcg agagattcta
     4441 agctatctct tatcgccgtt gctccgctac cggcaggaga ccttgcgcga gcggtgaagc
     4501 gctcccatgc gccccttgaa ggaatgaact cccacgaagt cgagaggatc gatgaatatt
     4561 tcttcgaaag tgccgctcgt catcaagctt gaaccatcca agatcggatc tcgcggccct
     4621 gcttcgaact cagattaaaa acaatcaacg gacctccgcc tcagccaagc ttgccagctg
     4681 tacgggatga cgattcgaag aaaagtcatc ccgctctagg tgtggagcct cgaccgccgt
     4741 ctctggcgcc gtttcgcgcc aatgagcgat caagccagtg tttcgtccag ccagtcgaag
     4801 atgcgggcga aagccaggcg gcccgcgccg acttggcagt gggcgccggc gccttcggct
     4861 ttggtaaagc gcatcatcgt cttgcggcag gtcagatggt cataaagctg ttgtggttgg
     4921 cccttgaaga agaggtcgtc ctccgcatca cacaccaggg tcgggcagct tatggcctcg
     4981 gcgacgctcg tcgtaccagt agtcaccgga cttgatgcgg gccgaggtgg ccaggacttc
     5041 gccgaactgc gcaccgccat attctgcggc tccgaaagcg cgctgggtct cgtaccagaa
     5101 ttgaacgtcg tctgggaaca gaggcttctt gtccatcgcc gtcggggcca atggcgcagc
     5161 cccggatggc gtgtcctccg cgcgcgcacc gctggcagcc atggtcgcga gcgggatccc
     5221 aagtgccaaa agtgccctgc gattcatcga cgatcctctc aaacgggcta cgatcgctgg
     5281 tggtcccttg attccaatgt tcgcaaagac cttcgccgaa acgggatgcg aatgttagaa
     5341 acggaccgcc acgtcagtgc gacaggtgta tcattaacgc aaacattgct gcaataagga
     5401 atgaggcatg acagcgaaac cccgccccgg tggccgcagt gcccgcaaca aggcggcggt
     5461 tttcgaagcc gcggccgccc tgctggttga acggggacac gaggcggtca ccatgaccga
     5521 tatcgccgag cgcgccggga tggccgcgac cagcctctat cgccgctggg gcgacgtgcg
     5581 cgcgttgatg atggaggtcg cggtcgaaaa attgatgcgc gagcggccgc tgcccgatac
     5641 cggatcgctg cgcggcgacc tgctcgcctg ggcccgcccg atcgcaacca gcctcgcgag
     5701 ccgcgaaggc tcgtcgttct ttcgggccgt gatcgcaaca acgacgccgg ccggtgccga
     5761 tggatcattg cgccgcgcgg cgctgaaagg ccggagcgag cagatggagc tgatgctgga
     5821 gcgcgcccgg cagcgtggcg agaaaccgcc tggcgtcgcc gagctgttgg attatgtgct
     5881 ggcgccgctt tacatgcgcg cgctgtttgg caggccgctc agcaaggccg tcgccgatcg
     5941 tctggtcgat cggctggtgg cgcgatccta gcgttcgcct tgcaaagccc ccgtatcact
     6001 ccccactagt aacgcaaatg tcattgcgtt aattaaattc ttaatgcaac atttatggca
     6061 ttaagtgcta tcggcattgc tgcggcagat cacgcaacgc cgagggaaat acgatggcaa
     6121 agctcagcat caacggacag acagtcgata tcgacgtcga gccggacacg ccgctgcttt
     6181 gggcgatccg tgaaaacatc gggctgaccg gcaccaaata tggctgcggc atcgcccaat
     6241 gcggcgcctg cacggttcac atcgatggcg tcgcggtgcg atcctgcggc gtgcaggtca
     6301 gcgaagccgt cgccaagcgg atcaccacga tcgagggact cgccgtcaac ggcgcgttga
     6361 ccaaggtgca gcaagcctgg atcgataacg acgttccgca atgcggctat tgccagagcg
     6421 gcatgatcat ggcggtcacg gctctgctca aggagaagcc caagccgacc gacgctgaca
     6481 tcgacgacgc cattaccaac atctgccgct gcggcacttt ccagcaggtg cgtgaagcca
     6541 ttcacgccgc cgcgaacgct tgaggaagat gccatgaccc atatgcccca actcaatcgc
     6601 cgcgcttttg tcgtcggcgc cgccgcagcc ggcgctggtc tcgcccttgg attcgacgtc
     6661 ccgtttggtg gaccgaccgt ggttcgcgcc gccgatggca cgccggaggt caatgcctgg
     6721 gtggtgatcc ggcccgatga caccgtggtg atccgcatcg cccgctccga gatggggcaa
     6781 ggcacgctga cgggcctcgc tcaactcgtc gccgaagaac tcgaatgtga ctggtcgaaa
     6841 gtgaccaccg aatatccgac cccgggacaa agtgccgcgc gcaagcgcgt gtggggcagc
     6901 ttcagtaccg gcggcagcat cggcgttcgt gcctcgcagc aagatgtccg caagggcggc
     6961 gcgacagccc gcatgatgct ggttcaagcg gccgcgaatg aatggaaagt gccggttgcg
     7021 gaatgcaccg ccgccaacag tgtcatcacc catacgccgt ccggacggac catcagcttt
     7081 ggcaaggtgg cggaagcggc cgcgcgggtt gagccgccgg tcgaggtcaa gctgaaagac
     7141 cccaaggact ggaagatcgc cggcaaaccg ctcaagcggc tcgatacatc agacaagacc
     7201 accggcaaga tgatctacgg catcgacgtc aaactgcctg gcatgctcaa cgccgcgatc
     7261 aaggattgtc ctgtgtttgg cggcaagctg aaaagcttcg acgaagccaa gatcacgggc
     7321 atgaagggtg tcaagaaagt cgtacgtgtc ggtgacagcg ctgtcgctgt cgttgccgac
     7381 acctggtggc gcgccaaaac cgcgctggac gctttgccgg tcgtttggga cgaaggcgac
     7441 aatgccaagg tatcgagcgc atcgatcgcg aaatggcttg aggaaggtct cgatcctgta
     7501 cagcctgcat ttgtcggcaa cgccaacggc gatgccaaag ccgcgatcgc cagtgccgcg
     7561 aaaaagatag aagcggtgta cggctatccc caccagaacc atgcggcgct ggagccgctt
     7621 aacgccaccg cgctctacac gcctgacaag tgcgaggtct ggaccggcac ccaggatgcc
     7681 gaactggcct acacggtgac ggctaaggtc tcgggcctgc ctcccgaaaa atgtgacatc
     7741 cacaaagtga tgctcggcgg cggcttcggc cgtcgcaacg cacctgacta tgtccggcag
     7801 gcggtcctga tcgccaagca gatgccgggc acgccggtca aactgctgtg gtcacgcgaa
     7861 gaagacatga cgcacggcaa atatcatccg gtgacccagt gcaagttgac cggcgcattc
     7921 gatgccgaca ataatctgac ctgcctgcat atgcgaatct cgggccaatc ggtttttgcc
     7981 agcatcactc cccaggtgtt gaaagacggc atggacccgt tcacgttcca gggcgtcgcc
     8041 cccaccggtg aggcgttcgc cagcatcgga ccggcgcggc tgggttacac catcccccat
     8101 ttgctgatcg accattccat gcgcaatccg catgtgccgc ccggagtatg gcgcggcgtc
     8161 aacggcaacc agaatggctt ctatctcgaa tgcttcatgg atgagctggc gcatgcggcc
     8221 gggcaggacc cgctcgaatt ccgccgcaag ctgatggcgg atcatcccaa gcatcttgcc
     8281 gtgctcaacg cggtcgcgga gcgcgtcggc tggagcaagc ccgcgccgca agggatattt
     8341 cgcggcatcg cccagcagat ggggcttgga agttacgtcg ccgcggccgc cgaaatctcc
     8401 gtcaccgacg gcaacaagat caaggtgcat cgcatcgtcg ccgccatcga ttccggcacc
     8461 gtcgtcaatc cggcacaggt cgaacgacag gtcgcgggct cattcgtcta tggcctgtcg
     8521 gcgctgttct atggcgaatg cacggtcaag gatggccgta tcgagcagac caattttgat
     8581 acctacaact cgatgcgtat cagcgagatg ccgaaggtgg agacgatcgt gatgccgagc
     8641 ggtggattct ggggcggcgt cggcgagccg acaattaccg tcgcggcgcc tgctgtgctc
     8701 aacgcctact tcgcggcgac aggcaagcgc atccgctcgg tgccgctgaa gaaccaggat
     8761 attgctttcg cttggtgaag ctgcagcggc agttgcttcg ccaaggaaaa gttgcgatca
     8821 catcgaacgg caaaacgaga gaaaccatca tgaaaatcgt tgatgcatca tgaaaatcat
     8881 tgatgcaccg ctgctgcttc ggactgccgc catcaccatc acgatcgccg ccagttactc
     8941 tgtggcacaa gggcaggtgg ctcccaaggg acccgagccc gcaaccttga aggcaaacgc
     9001 cgagttggca aagacattgc cgtttgcaaa ccggcaggat ttcgacgacg ctatgcgcgg
     9061 cttcatcggc accacgcccg acgcgctggt gacgggcacc ggtccgcgcc cggtgtggag
     9121 catgaagcct tacgacttcc tcaagcagaa cgaagtagcc gacagcgtca accccagtct
     9181 gtggcggcag gcgcagctca acgcgatcca cggcttgttc aaggtgaccg agcgcgtcta
     9241 tcaggtgcgc ggcttcgaca tctccaacat gacgatcatc gaaggcgaca cttccctgat
     9301 catcatcgac gtgctgctga cgaccgagac ggcgcgcgcc gcgctggagc tgtattacca
     9361 gcaccggccg aagaaaccgg tgggcacggt gatctattcg cacagccacg gcgatcactt
     9421 cggcggcgtc aagggcgtga tcagcgaggc cgacgtggtc gccggcaagg tccaggtgct
     9481 ggcgcccgca ggcttcttgg aagctgtcgc gggcgaaagc gtcatggcgg gtaccgcgat
     9541 gagccggcgc gcgcagtatc agttcggtcc gttgctgccg ccgggcccgc gcggccacgt
     9601 cgataccggt ttgggcaaga acgtcgcgcg cggaaccatc acattgattg cgcccaccgg
     9661 cacgatcgac aagaccatgg aaaagcgcgt catcgatggc gtggagatcg tctttcaact
     9721 ggcgcccgac tccgaggcgc catcggaaat gctgatgtac ttcccgcagt tccgcgtgct
     9781 cgacatggcc gaggatgtca cccacaacat gcacaacctc tacactattc gcggctccga
     9841 ggtgcgcgat ggcaatctgt ggtcgcgcta catcagcgaa gcgatggaga ccttcggcga
     9901 caggagcgac gtcatcatcg cgcagcatca ctggcccgtt gccggacagg cgcgcgtcgt
     9961 cgccctcatg aagaagcagc gcgacatgta caagttcatc aacgaccagt cgctgcggct
    10021 gttgaacctt ggctacacgc ccggcgagat cgccgagacc ctgcacatgc ccgcgagcct
    10081 ggaacaggaa tggtccgccc gcggctatta cggcacgctg cgtcacaacg ccaaggcggt
    10141 gtatcagaaa taccttggct ggtacgacgc caatcccgct gacctcgacc cgctgccgcc
    10201 ggccgagaac gcgcgcaaaa ccgtcgagta catgggcggc gctgatgcag tgatcgcgcg
    10261 cgcacgcggc gacttcgcca aaggcgaata ccgctgggtg gcgagcgcga tgaaccaggt
    10321 ggtgttcgcc gatcccgcta accgcgccgc gcgcgaattg ggcgccgacg cgctggagca
    10381 actcggcttt caggccgagg ccgccacttg gcgcaacgcc tatctcgtcg gcgcgatgga
    10441 gctgcgcaac ggcgt
//
LOCUS       DATNOI010000071         7051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2471802,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000071 DATNOI010000000
VERSION     DATNOI010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2471802"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..381
                     /locus_tag="VN968_02410"
     CDS             73..381
                     /locus_tag="VN968_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HXL28134.1"
                     /translation="MPKGCTHIAGIRNVTPSALGCEECLKSGSVWLHLRICRTCGHVG
                     CCDDSPNKHATKHFHATQHPIIEGYDPPEGWGWCYVDEVLFDLSNRKTPHNGPIPRYY
                     "
     gene            421..1338
                     /locus_tag="VN968_02415"
     CDS             421..1338
                     /locus_tag="VN968_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL28135.1"
                     /translation="MIKIFRAAGLAATVALSAALLDPVAAFAQAPQSKFAEVNGVRLH
                     YLVAGKGDPVVLLHGYAQTSHMWLPLIAKLADKHTVIAPDLRGFGQSSAPADGYTKAA
                     MAQDIHALVKSLKYDRIRLVGHDIGLMVAYAYAAQYPSEVDRLVLMEAFLPGVGDWNN
                     VFLLRDLWHFHFFGKTPLALVTGRERIYLEHFWNDFAADPKKSVLEKDREFYARAYAK
                     PGHMAAGMEVFRAFPKDAEDFAGFAKTRLSMPLLVLSGEKAGGPFLIEQGKMVATNVE
                     GVLVKGRGHWLMEEAPDQVIPKLVEFLNR"
     gene            1502..2014
                     /locus_tag="VN968_02420"
     CDS             1502..2014
                     /locus_tag="VN968_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28136.1"
                     /translation="MILGMSLSTFVTVHVIISLIAIVAGLVVMSGLLTSRSMPGLTAI
                     FLLFTILTSATGFLIPPLLSEKLLPSHIVGALSLVLLAIACIALYGMKLSGAWRWIYA
                     VTALLSLYLNVFVLAIQGFLKVPALHALAPGEPPGGPVFAVVQLIVLVFFVIVIIGAV
                     RRFRPMPAFA"
     gene            2181..3005
                     /locus_tag="VN968_02425"
     CDS             2181..3005
                     /locus_tag="VN968_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL28137.1"
                     /translation="MPTITTKDGVEIFYKDWGKGQPIVFSHGWPLSADDWDTQMLFFL
                     NHGYRVIAHDRRGHGRSSQPGDGHDMDHYADDLAALTAHLNLKDAIHVGHSTGGGEVV
                     HYLGRHGESRVAKAAIISAVPPLMVRTPANPGGLPKEVFDGLQVQLAANRSQFYRDLP
                     EGPFYGFNRPGVKASEAIIQNWWRQGMMGGAKAHYDGIVAFSQTDFTDDLKKITVPVL
                     VMHGDDDQIVPYADAGPLSAKLLKNGTLKTYKGFPHGMPTTQAATINADLLAFLKG"
     gene            3309..4598
                     /gene="murA"
                     /locus_tag="VN968_02430"
     CDS             3309..4598
                     /gene="murA"
                     /locus_tag="VN968_02430"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXL28138.1"
                     /translation="MDRIRIVGGNKLNGTIPISGAKNAALPLMIAGLLTEETLVLDNV
                     PRLADVAQLQRILGNHGVDIMSAGKRPGDHEYQGQTLHISAANIIDTTAPYELVSRMR
                     ASFWVIAPLLARMHQAKVSLPGGCAIGTRPVDLLIMALEKLGAKIAIDGGYVLAMAPG
                     GLKAANIEFPKVTVSGTHVAVMAATLAKGTTVIANAACEPEIFDVASCLNKMGANVFG
                     AGTPRIVIEGVEKLHGARHTVLPDRIETGTYAMAVAMTGGEVQLAGARPELLQSALDV
                     LTQAGAEISVNNQGIRVARNGASIGPVTVSTAPFPGFPTDLQAQLMALMACANGSSHI
                     TETIFENRFMHVQELARFGARISLDGETATIEGIPKLRGAPVMATDLRASVSLVIAGL
                     AAEGETMVNRVYHLDRGFERLEDKLSACGATIERISG"
     gene            4686..5141
                     /locus_tag="VN968_02435"
     CDS             4686..5141
                     /locus_tag="VN968_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HXL28139.1"
                     /translation="MAPQLKLIALDADDLAVISTHVQDARVQTADIIWRQGEKRLVVG
                     MSRLDWEQTLAGETAPRRLIAALRFDRVLACKARNIDVEAPEAALELVGIEFHPGDAP
                     SGSALLMFSQGGALRLDVECLECELTDLGTDDLGTADIGDGAGSPGHGS"
     gene            5373..6668
                     /gene="hisD"
                     /locus_tag="VN968_02440"
     CDS             5373..6668
                     /gene="hisD"
                     /locus_tag="VN968_02440"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXL28140.1"
                     /translation="MPVRLDSKSADFAERFASFLATKREISADIEAAARAIVDDVAAR
                     GDIALIEATRKFDRLDLDAIRLRVTAAEIEAAIKACDAKTLEALELARNRIESFHRQQ
                     LPHDQRFTDALGVELGWRWSAIESVGLYVPGGTAAYPSSVLMNAVPAQVAGVARVVMV
                     VPSPDGKLNPLVLAAARLGGVSEIYRVGGAQAIAALAHGTATIAPVAKIVGPGNAYVA
                     AAKRLVFGKVGIDMIAGPSEVLVISDQTGNAGWIAADLLAQAEHDVNAQSILVTDSAP
                     LAEEVERAVQSQLATLPRAQIARASWNDFGAVILVKNLGEAADLANAIAAEHLEIMTS
                     DPETLAAQIRNAGAIFLGPHTPEAIGDYVGGSNHVLPTARSARFSSGLGVLDFMKRTS
                     ILKCGPDQLRALGPAAMTLGEAEGLEAHARSVGLRLNQS"
     gene            6665..>7051
                     /locus_tag="VN968_02445"
     CDS             6665..>7051
                     /locus_tag="VN968_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HXL28141.1"
                     /translation="MTRPHRDEDSHNRIVAVTLDEESIGRSGPDIEHERAIAIYDLVE
                     QNLFAPEGADKGPFTLHIGITGNRLMFDIRREDGTPVVAHLLSLTPFRRIVKDYFMIC
                     DSYYEAIRTATPDKIEAIDMGRRGIHD"
BASE COUNT         1292 a         2190 c         2260 g         1309 t
ORIGIN      
        1 gcacaggtgg tggccgcgct gcacggtttc cttggcgata gggcgaagcc ggcgctttag
       61 gggatgatcg gaatgccaaa gggctgcaca cacatcgccg gcatccgcaa cgtcacgccg
      121 agcgcgctcg gttgcgagga atgcttgaag agcggcagcg tgtggctgca tttgcggatc
      181 tgccgcacct gcggtcacgt cggttgctgc gacgattctc ccaacaagca cgcgacaaaa
      241 catttccatg ccacccaaca tccgatcatt gaaggctacg atccgccgga aggatggggt
      301 tggtgctacg tcgatgaagt gctattcgat ctgtcgaacc gaaagacgcc gcataacggt
      361 ccgatcccgc gttattattg acggcgtgtt tcaatttgaa caagataccg ggggcaatcc
      421 gtgatcaaaa tatttcgtgc cgctggcttg gccgccacgg tggcgctctc tgccgccttg
      481 ctcgacccgg tcgcagcgtt tgcgcaggcg ccgcaaagca agtttgccga ggtcaacggt
      541 gtcaggctgc actatctcgt tgccggcaag ggcgatccgg tcgtgctgtt gcacggctat
      601 gcccaaacca gccatatgtg gctgccgctg atcgcaaaac ttgccgacaa gcataccgtg
      661 attgcgccgg atctacgcgg cttcggtcaa tcctcggcgc ctgcggatgg atacaccaag
      721 gcagcgatgg cgcaggacat ccacgcgctg gtgaagagcc tcaaatacga ccgcatccgc
      781 ctcgtcggcc acgacatcgg gctgatggtg gcctatgcct atgcggcgca atacccgagc
      841 gaggtcgatc ggctggtgct aatggaagcg ttcctgcccg gcgtcggcga ctggaacaac
      901 gtctttctgt tgcgcgatct ctggcatttc catttcttcg gcaagacgcc gctggcgctg
      961 gtcaccgggc gtgaacggat ttatctcgag catttctgga atgattttgc cgccgatccg
     1021 aaaaaatcgg tgctggaaaa agaccgcgaa ttttatgcca gggcctatgc caagcccggc
     1081 cacatggccg ccgggatgga agtcttccgc gcttttccca aggacgccga agacttcgcg
     1141 ggatttgcca agaccaggct gtcgatgccg ctgctggtgc tatcgggcga gaaagccggc
     1201 ggtccgttcc tgatcgagca gggcaagatg gtggccacca atgtcgaggg cgtgctcgtc
     1261 aaaggccggg gccattggct gatggaagag gcgccggacc aggtcatccc gaaactggtc
     1321 gaatttctca atcgctagaa tgcggaagca atgcgggcgc ggccagccat gcaaaaaact
     1381 tgggcatcgc tatcgcgttt ttgatcgtcg ccatatcaga attgccgctg tgcgctgagc
     1441 gcaggtggca attttcaatc cgcagtcgta gcattcgccg gaccaaaaag gggagcgtgt
     1501 catgattttg ggtatgagcc tgtctacttt tgtcaccgtg cacgtgatca tcagcctgat
     1561 cgcgatcgtt gccggccttg tcgtcatgtc cgggcttctc acctccaggt cgatgccggg
     1621 gctgactgcg atattcctgc tgttcacgat tttgaccagc gccaccggct ttctgattcc
     1681 gcctctgctg tccgagaagt tgctgccgtc gcacatcgtc ggcgctctct cgctcgtgct
     1741 gctggcgatc gcgtgtatcg cgctctacgg catgaagctt tccggtgcgt ggcgctggat
     1801 ctatgcagtg acggcgctgc tgtcgcttta cctcaacgta ttcgttctgg cgatccaggg
     1861 attcctgaaa gtgcctgcgt tgcatgcgct ggcgccggga gagccgccgg gcgggccggt
     1921 gttcgccgtc gtgcagctca tcgtgcttgt cttcttcgta attgtgatca tcggtgccgt
     1981 gcggcgattt cggccgatgc cggcattcgc atgatgtccg cgaattcgat ctaaccacgc
     2041 ttcgtcattc cgggacacgc gtctttgcgt ggacccggaa tccagatgta tcttgatcga
     2101 gattccagga tcgacgctgc gcgtcgcccc ggaatgacgg agcgggatgc atcgccatcg
     2161 tcccacaaag gagccctgcg atgcccacga tcacgaccaa ggacggtgtt gagattttct
     2221 acaaggactg gggaaaaggt cagcccatcg tgttcagcca cggttggccg ctgtcggccg
     2281 atgactggga cacccagatg ctgttcttcc tcaatcacgg ctatcgagtg atcgcccatg
     2341 accgccgcgg ccacggccgc tccagccagc ccggcgacgg ccatgacatg gatcactatg
     2401 cggacgatct tgcggctttg accgcgcatc tcaatctgaa agacgccatc catgtcgggc
     2461 attccaccgg cggcggcgag gtcgttcact atctgggacg gcacggcgag agccgggtgg
     2521 cgaaggcggc catcatcagc gcggtgccgc cgctgatggt gaggacgccg gccaatcccg
     2581 gcggccttcc gaaggaagtg ttcgacggat tgcaggttca gctcgcggcc aatcgctcgc
     2641 aattctatcg tgacctgccg gagggtccgt tctatggctt caaccggccg ggcgtgaaag
     2701 cctcggaggc gatcatccag aactggtgga ggcagggcat gatgggcggc gccaaggcgc
     2761 attacgacgg catcgtcgcg ttctcgcaga ccgatttcac cgacgacctc aagaagatca
     2821 ccgtgccggt gctggtgatg cacggcgacg acgatcagat cgttccctac gccgacgccg
     2881 ggccgctgtc ggcaaagctt ttgaagaacg ggacgctgaa gacctacaag ggctttccgc
     2941 acggcatgcc gaccacgcaa gcggccacca tcaacgccga cctgctcgcg ttcctcaagg
     3001 gatgaaagag aatcgtcatt cccgcgcaag cgcgtagcgc ttgtcgctga gggcgcgcga
     3061 agcgcgaacc cggaatccat aaccaccatc gggggtatgg attccgggcc tgcgcatcca
     3121 agtcggctgt tgccgacttg gataatgata ttgccgaact cgggtagacc cgagttcggt
     3181 ggcgcacccc ggaatgacgg cggactggcc ggcgccggcc cggaccccat caaatagccg
     3241 caaatttcac gcttttgtcg tcagggccgt tggccctata ctttgcgcat tggcaaaagc
     3301 ggggattcat ggatcgcatt cgcatcgtcg gcggcaacaa gctcaatggc accattccga
     3361 tctcgggggc gaagaatgcc gcgctgccgc tgatgatcgc cgggcttttg accgaggaaa
     3421 cgcttgttct cgacaacgtg ccgcggctgg ccgacgtcgc gcagctgcag cgcattctgg
     3481 gcaatcacgg cgtcgacatc atgtcggcgg gtaaacgccc cggcgatcac gaataccagg
     3541 gccagaccct gcacatttcg gccgccaaca tcatcgacac caccgcgccc tacgaactgg
     3601 tgtcgcggat gcgcgccagc ttctgggtga tcgcgccgct gctggcgcgg atgcaccagg
     3661 ccaaggtatc gctgcccggc ggctgcgcca tcggcacaag gccggtcgat ctgttgatca
     3721 tggcgcttga gaagctcggc gcgaaaatcg ccatcgatgg cggttatgtg cttgcaatgg
     3781 caccgggcgg cctcaaggcc gccaacatcg aatttcccaa ggtgacggtg agcggcaccc
     3841 atgtcgccgt gatggcggca acgcttgcca agggcacgac cgtcatcgcc aatgccgctt
     3901 gcgagccgga aatcttcgat gtcgcatcct gcctgaacaa gatgggtgcg aacgtcttcg
     3961 gcgccggtac gccccgcatc gtcatcgaag gcgtggagaa gcttcacggc gcacggcaca
     4021 ccgtgctgcc ggaccggatc gaaaccggca cctatgcgat ggcggtggcg atgaccggcg
     4081 gcgaggtgca actggccggc gcgcgccccg agcttctgca atcggcgctc gacgtgctga
     4141 cgcaggctgg cgcggagatc tcggtcaaca accagggcat ccgcgtcgcc cgcaatggag
     4201 cctcgatcgg tccggtgacg gtttcgaccg cgccgtttcc gggctttccc accgatctgc
     4261 aggcgcagtt gatggcgctg atggcctgcg ccaacggctc ctcgcatatc accgagacga
     4321 ttttcgagaa tcgcttcatg catgtgcagg agctggcgcg gttcggcgcg cggataagcc
     4381 tcgacggcga aaccgcgacc atcgagggca ttcccaaact gcgcggcgcg cctgtgatgg
     4441 cgaccgatct gcgcgcctcg gtctcgctgg tgatcgcggg gctcgccgcc gaaggcgaga
     4501 ccatggtcaa ccgcgtctac cacctcgacc gcggctttga gcggctcgag gacaaattgt
     4561 ctgcctgcgg cgccaccatc gagcgcatca gcggatgaac tttttggccc cgtattcagt
     4621 tggggcgtgg tctcgggctt gacccgaggg taggtacgcg cagacgaatg accaggtggg
     4681 aatcggtggc gcctcagctc aaattgattg cgctcgatgc cgacgacctc gcggtgattt
     4741 cgacccatgt ccaggatgcc cgcgtgcaaa ccgccgacat catctggcgg cagggcgaaa
     4801 agcggttggt ggttggcatg agccggctgg actgggagca gacgctggcc ggcgagaccg
     4861 ccccgcgccg cctgatcgcg gcgctgcgct tcgaccgggt gctggcgtgc aaggcgcgca
     4921 acatcgacgt ggaggcgccg gaagcagccc ttgagctggt cgggatcgaa tttcatcccg
     4981 gcgatgcacc cagcggcagc gcgcttttga tgttcagcca gggcggcgcg ttgcggctcg
     5041 acgtcgaatg cctggaatgc gagctgaccg atctcggcac cgacgatctc ggaacggccg
     5101 atatcggcga cggcgccggg tcgccggggc acggtagttg aaatttatcg tcgtccccgc
     5161 gaaagcgggg acccataacc accgctgtaa gcggggccca ggctagcggc cccagcgtgc
     5221 catggcatga aagagcggtg gttatgggtc ccggcgcgtt ggccgggacg acgtcttatt
     5281 actggaccga catagcgcgg tattcgtatg gcaaagggtt gacggcggtt tgtggccgcg
     5341 ccattgagca gtgacgcttc cggaaagcca ccatgcctgt ccgcctcgat tcaaagagcg
     5401 ccgatttcgc cgaacggttc gcgagctttc ttgcgaccaa gcgcgagata tccgccgaca
     5461 tcgaggccgc ggcccgcgcc atcgtcgatg acgtcgcggc gcgaggcgac atcgcgctga
     5521 tcgaggcgac acgcaagttc gaccggcttg atctcgatgc catcaggttg cgcgtcaccg
     5581 cggccgagat cgaggcggca ataaaggcct gcgacgccaa aacgctcgag gcgctcgagc
     5641 tcgcccgcaa ccggatcgaa agcttccatc gccagcaatt gccgcacgac cagcgcttca
     5701 ccgatgcgct cggcgtcgag ctcggctggc gctggagcgc gatcgaatcg gtcggcctct
     5761 acgttcccgg cggcaccgcc gcctatccat cctcggtgct gatgaatgcg gtgccggcgc
     5821 aggtcgcggg cgtcgcgcgc gtggtgatgg tggtgccgtc gccggacggc aagctcaatc
     5881 cgctcgtgct ggcggcggcg cgtctcggcg gcgtctcgga aatctaccgc gtcggcggcg
     5941 cgcaggcgat cgcggcgctc gcccatggca ccgccaccat cgcgccggtc gcaaagatcg
     6001 tcggccccgg caatgcctat gtcgccgcgg caaaacggct ggtgttcggc aaggtcggca
     6061 tcgacatgat cgccggcccc tcggaagtgc tggtgatatc ggatcaaacc ggcaatgctg
     6121 gctggatcgc ggccgatctg ctggcgcagg ccgagcacga cgtcaacgcg caatcgatcc
     6181 tggtcaccga cagcgcaccg cttgccgagg aggtcgaacg cgcggtgcaa agccaacttg
     6241 cgaccctgcc gcgcgcgcaa atcgcgcggg cctcgtggaa cgatttcggc gcggttattc
     6301 tcgtgaaaaa tctcggcgag gccgctgatc tcgcgaacgc gatcgccgcc gagcacctgg
     6361 aaatcatgac atcggatccg gaaaccctcg cggcgcaaat ccgcaatgcc ggcgcgatct
     6421 tcctcgggcc ccacacgccg gaggcgatcg gcgactatgt cggcggctcc aaccacgtgc
     6481 tgccgaccgc gcgctcggcg cgattctcgt cggggcttgg cgtgctcgac ttcatgaagc
     6541 ggacctcgat cctgaaatgc gggccggacc agctgcgggc gctgggaccg gcggcgatga
     6601 cgcttggtga ggccgaaggc ctcgaagccc atgcgcgttc ggtcggactg cgcctcaacc
     6661 agtcatgacc aggccgcacc gagacgagga ttcgcacaat cgcatcgtcg cggtgacgct
     6721 ggacgaggaa tcgatcgggc gctccggccc cgatatcgag cacgagcggg caatcgcgat
     6781 ctacgacctg gtcgagcaga atctgtttgc gccggagggc gccgacaaag ggccgttcac
     6841 gctgcatatc ggcatcaccg gcaaccggct gatgttcgac atccgccgcg aggacggcac
     6901 gcccgtggtc gcgcatctgt tgtcgctgac gccgttccgg cgaatcgtga aagactattt
     6961 catgatttgc gacagctatt acgaggcgat ccgcaccgcg acgccggaca agatcgaggc
     7021 catcgacatg ggccgccgcg gcattcacga c
//
LOCUS       DATNOI010000072         2085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2115732,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000072 DATNOI010000000
VERSION     DATNOI010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2115732"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VN968_02450"
     CDS             <1..404
                     /locus_tag="VN968_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HXL28142.1"
                     /translation="RAGGQPFPHPGDKAYLLAPSGAEGPGFLMLQNFRVIMKYNPAEA
                     YALAIGHFADRLRGGAPFVQPWPRQERELTRAERLELQQLLAQRGFYRGTPDGQLGGQ
                     TREALRGFQASIGAPADGFASSDVLERLRGR"
     gene            560..>2085
                     /locus_tag="VN968_02455"
     CDS             560..>2085
                     /locus_tag="VN968_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH family hydrolase"
                     /protein_id="HXL28143.1"
                     /translation="MPKTKSFLRVFTEPGPLVALAVAVALLLGIAGPASAQFFNFGNN
                     FGNFGGPQRPPPPRGGGYGGGGGWFGGDFFAPFQQQAPKKVIREDFSKAPPPEKRETV
                     PERNVLVLGDAMADWLAYGLEDAYAERPDMGVIRKHKTVSGLIKYQPKGDPADWPAAA
                     KGLFATEKPDAIVVMLGLNDRMAIREPAVDKSDSKSSDKKSDKKDAKAKPEAKPADAK
                     SGAKPDGATDTAAKPDDKAVDTELSPDDAADNNDAPPVTTPEKSARSPNGLYEFREER
                     WVELYTRKIDEMIGVVKSKGVPVLWVGLPALRGAKATADMLFLDSLYREAASKAGITY
                     VDVWDGFVDEAGRFLQQGPDFEGQIRRLRSYDGVYFTRPGARKLAHYVEREITRLLAA
                     RSAPIALPTEPATPDANALPGQPAPRPLAGPILPLVASSVGTDQLLGGPGSRPAAVDA
                     LAARTLVKGEPLAAPAGRADDFVWPRREVGREQAKGETPSASVSPDGTATAPGAPPKP
                     "
BASE COUNT          369 a          690 c          692 g          334 t
ORIGIN      
        1 agcgcgccgg cggacaacct tttccccacc cgggcgacaa ggcctatctg ctggcgccct
       61 ccggcgccga gggcccgggt tttctgatgc tgcagaattt ccgggtgatc atgaaataca
      121 atccggcaga ggcttatgcg ctggcgatcg gccacttcgc cgaccgcttg cgcggcggcg
      181 cgccgttcgt gcagccatgg ccacggcagg aacgggagct gacgcgcgcc gagcggctgg
      241 aattgcagca gcttctggcg cagcgcggct tctatcgcgg cacccccgac ggccagctcg
      301 gcggccagac gcgcgaggcg ctgcgcggct tccaggcctc gatcggggcg cctgcggacg
      361 gatttgcctc ctccgacgtg ctggagcggc tcagggggcg gtgacaaggc cggtctccgt
      421 cccgaaagta accctgaatc aggcatttcc ccggcgagct tgacgggggc ggccaatgcc
      481 cgatttatgg taaggaatct gcccgcttgc ggcctgattc cgctataata gtggttgcca
      541 tccattacgg ccgagccgca tgccaaagac aaaatccttt ctgcgcgtgt tcaccgaacc
      601 cggtccgctg gtcgcgctgg cggttgcggt cgcgctactg ctcgggatcg ccggaccggc
      661 ttccgcgcag ttcttcaatt tcggcaataa tttcggcaat ttcggtggac cgcagcggcc
      721 gccgccgccg cgcggtggtg gatacggcgg tggcggcggc tggttcggcg gcgatttttt
      781 tgcgccattc cagcagcagg cgccgaaaaa ggtgatccgc gaggattttt ccaaggcgcc
      841 gccgccggag aagcgcgaga ccgtgccgga gcgcaacgtg ctggtgctcg gcgatgcgat
      901 ggccgactgg ctcgcctacg gtctcgagga tgcctatgcc gagcggcctg acatgggcgt
      961 gatccgcaag cacaagaccg tctccggtct catcaaatat cagcccaagg gcgatccggc
     1021 cgattggccc gccgccgcca agggcctttt cgccaccgaa aaaccggacg ccatcgtcgt
     1081 gatgctcggg ctcaatgacc gcatggcgat acgcgagcct gccgtcgata aatccgacag
     1141 caagtcatcc gacaaaaaga gcgacaagaa ggacgccaag gccaaaccgg aggccaagcc
     1201 cgcagacgcg aaatccggcg cgaagccgga tggcgcgacc gatactgccg caaagcccga
     1261 cgacaaagcc gtcgacaccg aattatctcc ggacgatgcc gccgacaata acgatgcgcc
     1321 gcctgtcact acgccggaga agagcgcgcg ctcgccgaac ggcctttatg agttccgcga
     1381 ggaacgctgg gtcgaactct acaccaggaa aatcgacgag atgatcggcg tcgtgaaatc
     1441 aaagggtgtg ccggtgctgt gggttggcct gcccgcgctg cgcggcgcca aggcgacggc
     1501 ggatatgttg ttcctggatt cgctgtatcg cgaggccgcc agcaaggccg gcataaccta
     1561 tgtcgatgtc tgggacggat ttgttgacga agcgggccgc ttcctgcagc agggcccgga
     1621 cttcgagggt cagatccggc gcttgcgctc ctacgacggg gtgtatttca ccaggcccgg
     1681 cgcacgtaag ctcgcgcatt atgtcgagcg cgagatcacc cggctgctcg ctgcccggtc
     1741 cgcgccgatc gcgctgccga ccgaaccggc aacgcccgac gccaatgcgc tgccggggca
     1801 gccggcaccg cgtccgctgg ccggaccgat cctgccgctg gtggcatcct cggtcggcac
     1861 cgaccaattg ctcggcggcc ccggctcgcg cccggccgcg gtcgatgcgc tcgccgcgcg
     1921 aacgctggtc aagggcgagc cgctcgcagc gccggccggc cgagccgatg atttcgtgtg
     1981 gccgcgccgc gaagtcggac gcgaacaggc gaagggcgaa actccatcgg cgtccgtttc
     2041 gcctgacgga acggcaacgg cacccggcgc accgccgaag ccgaa
//
LOCUS       DATNOI010000073        10279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1028890,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000073 DATNOI010000000
VERSION     DATNOI010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10279
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028890"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..946)
                     /gene="argF"
                     /locus_tag="VN968_02460"
     CDS             complement(20..946)
                     /gene="argF"
                     /locus_tag="VN968_02460"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HXL28144.1"
                     /translation="MSKALRHFLDLSELPTKELRNMLALSVAMKAKLKAHAEPLKPLE
                     GKTLAMIFEKPSTRTRVSFDVGMRQLGGEAIMLTGAEMQLGRGETIADTARVLSRYVD
                     AIMIRILNHDALLELAEHATVPVINGLTRRSHPCQVMADLMTFEEHRGPIEGRTIAWT
                     GDDNNVLASWAHAAERFRFKLNVATPPQLAPNKSMRDWIKATQAPIVLGNDPEAAVRS
                     ADCVVTDTWFSMGDKDGEHRHNLLKPYQVNAKLMSLAKPDALFMHCLPAHRGDEVTDE
                     VIDGPQSVVFDEAENRLHAQKGILAWCFEAVA"
     gene            complement(943..2154)
                     /locus_tag="VN968_02465"
     CDS             complement(943..2154)
                     /locus_tag="VN968_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXL28145.1"
                     /translation="MTDSATSHLLPVFARVDLGFQRGEGAWLIATNGERYLDFTSGVA
                     VNALGHAHPHLVAALQEQAMKLWHMSNLFKSPDGEQLAARLCEASFADLVFFANSGAE
                     AMECAIKITRKHHAAKGHPERYRIITFDSAFHGRTLATLAATGSAKYLEGFGPPLDGF
                     DQVTLGDLEAVRRAIGPHTAGILIEPLQGEGGVRAAPHAFFKALRQLCDDNGLLLIFD
                     EVQTGMGRTGDLFAYQRIGVTPDVMPLAKALGGGFPTGACLATAEAAAGMTPGSHGST
                     FGGNPLAVAAANAVLDVMLEPGFFEHVRKMSLLLKQKLASVVDRHPKVLAEVRGEGLL
                     IGLKAVVPSVDLVAALREQKLLTVGAGENVVRFLPPLIVTEAEIEESVARLERACVAL
                     SGDQLKRAAGK"
     gene            2583..3092
                     /locus_tag="VN968_02470"
     CDS             2583..3092
                     /locus_tag="VN968_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HXL28146.1"
                     /translation="MTVLTWTDDRVEQLKKLWESGLSASQIAAELGNVTRNAVIGKVH
                     RLGLSGRAKSPSSAAPRQRKARPAQHMMRVARPVSRGNTALAHAFEVEMEPDPIAFDN
                     VVPISQRLSLLELSEATCHWPIGDPSSSEFFFCGGKALSSLPYCAHHSRIAYQPASDR
                     RRQPPKPTR"
     gene            complement(3289..3990)
                     /gene="phoB"
                     /locus_tag="VN968_02475"
     CDS             complement(3289..3990)
                     /gene="phoB"
                     /locus_tag="VN968_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HXL28147.1"
                     /translation="MSARILVVEDEEALTTLLRYNLDAEGYDVETVGRGDDADTRLKE
                     RVPDLIVLDWMLPGLSGIELCRRLRARPETRSLPIIMLTARGEESERVRGLATGADDY
                     IVKPFSVPELLARVKGLLRRASPERLATVLTYGDIELDREKRRVARSGRPIDLGPTEY
                     RLLEFFLEHPGRVFSREQLLDSVWGRDIYIDERTVDVHIGRLRKLLNPGREQDPIRTV
                     RGAGYALDDRFAKVE"
     gene            complement(4088..4804)
                     /gene="phoU"
                     /locus_tag="VN968_02480"
     CDS             complement(4088..4804)
                     /gene="phoU"
                     /locus_tag="VN968_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HXL28148.1"
                     /translation="MASEHTAKAFDSDLQELTRLVAEMGGLAERMIVDSVDALVRRDI
                     ALGKRVVTNDVEIDNLQRVIEERAVLTIARRQPMAIDLREIVGAMRVATDLERIGDLA
                     KNMGKRVAALENDFHPLKLIRGLEHMTDLVQSQVKSVLDAYAAHDLPAAMSVWKGDEE
                     VDAICTSLFRELLTYMMEDPRNISFCIHLMFCAKNIERIGDHATNIAETVFYMIEGQQ
                     ILDKRPKGDMTTFATTVPGN"
     gene            complement(4815..5639)
                     /gene="pstB"
                     /locus_tag="VN968_02485"
     CDS             complement(4815..5639)
                     /gene="pstB"
                     /locus_tag="VN968_02485"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HXL28149.1"
                     /translation="MNDLSVSVSVPNAPVPSMAGHAEAPAKVTARNLNFYYGEHPALK
                     NINLTLGTHRVTAFIGPSGCGKSTLLRIFNRMYDLYPGQRATGQLMLDQTNILDPKLD
                     LNLLRARVGMVFQKPTPFPMTIYENIAFGIRLYEKISRSEMDGRVEKALRGGALWNEV
                     KDKLNASGLSLSGGQQQRLCIARTVAVRPEVILFDEPCSALDPISTARIEELIQELAE
                     DYTIAIVTHNMQQAARVSDKTAFMYLGELIEFDETNKIFTSPSDRRTQDYITGRFG"
     gene            complement(5636..6481)
                     /gene="pstA"
                     /locus_tag="VN968_02490"
     CDS             complement(5636..6481)
                     /gene="pstA"
                     /locus_tag="VN968_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HXL28150.1"
                     /translation="MNPIYTRRRRVDLIIRGLCFAAALFGVTWLALILFTLLFNGIAG
                     LNVQIFTQNTPPPGSSEGGLLNAITGSVIMTVIGVGIGAPLGLFAGTYLAEYGRNDRL
                     TSVIRFINDILLSAPSIIIGLFIYGAIVVPMGGFSAFAGCLALAVIVIPVVVRTTEDM
                     LLLVPNPLREAASALGLPRSLVIKRIAYRAARAGLITGVLLATARVAGETAPLLFTAL
                     SNQFFSLDLTKTMANLPVTINNFVQSPYAYWKQLAWSGALIITLTVLALNIGARILGA
                     ERTAK"
     gene            complement(6487..7476)
                     /gene="pstC"
                     /locus_tag="VN968_02495"
     CDS             complement(6487..7476)
                     /gene="pstC"
                     /locus_tag="VN968_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HXL28151.1"
                     /translation="MAVQSDVAEAAGPYDRAKALNAFKLGDVTFYWITRICAISVLLI
                     LGGIILSLIVGAWPAMKAYGFAFLWTQRWAPSADPPVLGALGPMYGTLVTSFIAMLIA
                     IPVGLGIAIFLTELCPQWLRRPIGIAIELLAGIPSIIYGMWGFFVLGPFLAYTFQPFM
                     IGLFGGVPVLGSIFGGPPSYLSLFNAALILAIMVLPFITAISVDVFKTVPPVLKEAAY
                     GVGCTTWEVVRNVVIPYTRVGVIGGIMLALGRALGETMAVTFIIGNSFRISSSIFAPG
                     TTISAAIASEFAESDGLHQSGLILLGLLLFVLTFFVLAAARLMLMRLEQKAGN"
     gene            complement(7564..8574)
                     /gene="pstS"
                     /locus_tag="VN968_02500"
     CDS             complement(7564..8574)
                     /gene="pstS"
                     /locus_tag="VN968_02500"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HXL28152.1"
                     /translation="MNFVKAIVAAGLVAASTSAFAADITGAGATFPFPIYSKWADAYK
                     KETGNGLNYQSIGSGAGIKQIQAKTVTFGATDAPLKAEQLEKDGLVQWPMVMGAIVPV
                     VNLEGIKPGELVLSGEVLGDIYLGKITKWDDPAIAKLNAKLKLPTDAITVVRRSDGSG
                     TTFNFTDYLSKSNPEWKTKVGSGTAVEWPAGVGAKGNEGVAGNIGQTKNAIGYVEYAY
                     AKQNKLTYAALINKAGKTVQPTVEAFQAAASNADWAKAPGYYVILTDQPGEKSWPITA
                     ATFILMHKEPVDKAASAEAVKFFKWAFTKGDKMAEELDYIPMPEPVVKLIEKTWSADI
                     KS"
     gene            complement(8714..10015)
                     /locus_tag="VN968_02505"
     CDS             complement(8714..10015)
                     /locus_tag="VN968_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL28153.1"
                     /translation="MAIDESSSGSFFSPWPDRLRHSAIILLAAGLALAVLVAFGELSL
                     VRATAVFICIGAAALVPWRLHDAVASREDVRGVNPVEAAAVSAVVAGMPDPAVLLDRA
                     GRVIHLNAAAAQLAPALRKNELAQFALRSPEIITALREAIATTESRRATYLDHVPVDR
                     WMELIVTPVPVPTLFGGIDKCMLMTFHDQTPLRRVEEMRADFVANASHELRTPLAALS
                     GFIDTLQGPARDDVKARERFLGIMHTQATRMARLIDDLLSLSRVELSAHVRPDTLVDI
                     VPIIRQVVDGLEPLAGDRQVAIDIQLPDRPVVIAGDREELLRLFENLIENALKYGASG
                     GKIIVSLTAAMSAEGLPEIRVMVRDFGPGIAPEHLPRLTERFYRVDVGDSRAQGGTGL
                     GLSLVKHIVNRHRGRLLIESVPKNGATFTACFPQLRSPAAV"
     gene            complement(10048..>10279)
                     /locus_tag="VN968_02510"
     CDS             complement(10048..>10279)
                     /locus_tag="VN968_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HXL28154.1"
                     /translation="MLVGLWQMDREELLAGMLLFRVLYYLAPFVISVILLTFREVILG
                     ARLKRLRRARPGLDAEPKREAVYVRERGDTGA"
BASE COUNT         1919 a         3248 c         3239 g         1873 t
ORIGIN      
        1 cgaagctact accgcgcggt cacgccaccg cctcaaaaca ccacgccaga atgccctttt
       61 gcgcgtgcag gcgattttcg gcttcgtcga acacgaccga ttgcgggccg tcgatcactt
      121 cgtcggtgac ttcgtcgccg cgatgcgcgg gcaggcagtg catgaacagc gcgtcgggtt
      181 ttgccagcga catcagcttg gcgttgacct ggtacggttt caggaggttg tggcgatgct
      241 cgccgtcctt gtcgcccatc gagaaccagg tgtcggtgac cacgcaatcg gcgctgcgga
      301 ccgccgcttc cggatcgttg ccgagcacga tcggcgcctg ggtcgccttg atccagtccc
      361 gcatcgattt gttcggcgcg agctgcggcg gggtcgccac gttcaacttg aatcgaaacc
      421 gttccgccgc atgcgcccac gacgccagca cgttgttgtc gtcgccggtc caggcaatgg
      481 tgcggccttc gatcgggccg cgatgctcct cgaaggtcat caggtccgcc atcacctggc
      541 agggatgcga ccgccgcgtc agcccgttga tgacgggcac ggtggcgtgc tccgcaagct
      601 ccagcagcgc atcatggttg agaatgcgga tcatgatggc gtcgacgtag cgcgacagca
      661 cccgcgcggt gtcggcaatg gtctcgccgc gaccgagctg catttccgcg ccggtcaaca
      721 tgatcgcctc accgccgagc tggcgcatgc cgacgtcgaa cgacaccctc gtgcgggtcg
      781 agggcttttc gaaaatcatc gccagcgtct tgccttcgag cggcttgagc ggttcggcgt
      841 gcgccttcaa tttcgccttc atggcgacgc ttagcgcgag catgttccgc aactctttgg
      901 tcggaagctc gctgagatcg agaaaatgcc gcagcgcttt actcatttcc ccgccgcccg
      961 tttcaactga tcgccggaaa gtgcgacgca ggcgcgctcg agccgtgcca ccgattcttc
     1021 gatctccgcc tcggtcacga tcaacggcgg cagaaaccgc accacgtttt cgccggcgcc
     1081 gaccgtgagc agcttttgct cgcgcaatgc cgccaccaga tcgaccgacg gcaccaccgc
     1141 cttcaggccg atcagcaatc cttcgccgcg cacttcggcg agcaccttcg gatggcgatc
     1201 gaccaccgag gccagcttct gcttgagcag cagcgacatc ttccgcacgt gctcgaagaa
     1261 gccgggctcg agcatgacat ccagcaccgc attggccgcc gccaccgcca gcggattgcc
     1321 gccgaaggtc gaaccgtgcg agcccggcgt catgccggcg gcagcctcgg ccgtcgccag
     1381 gcaggcaccg gtcgggaaac caccgccaag cgctttcgcc agcggcatca catcgggcgt
     1441 aacgccgatg cgttgatatg caaacagatc gccggtgcgg cccatgccgg tctgcacctc
     1501 gtcgaagatc aacagcaggc cgttgtcgtc gcacagctgg cgcaacgcct tgaagaaggc
     1561 gtgcggcgct gcgcgtacgc cgccctcgcc ctgcagcggt tcgatcagga tgcccgcggt
     1621 gtgcggaccg atcgcgcgtc tgacggcttc gagatcgccg agcgtcacct gatcgaagcc
     1681 atccagcggc ggcccaaaac cctcgagata tttggcggac ccggtcgcgg ccagcgtcgc
     1741 cagcgtgcgc ccgtgaaacg cgctgtcgaa ggtgatgatg cgatagcgct ccgggtggcc
     1801 tttggcggcg tgatgtttgc gcgtgatctt gatcgcgcat tccatcgcct cggcgccgga
     1861 gttcgcgaaa aacaccagat cggcaaagct cgcttcgcac agccgcgccg cgagctgctc
     1921 gccgtccgga ctcttgaaca ggttcgacat gtgccacagt ttcatcgcct gttcctgcaa
     1981 cgctgcgacc agatgcggat gcgcgtggcc cagcgcattc accgcgacgc cagaggtgaa
     2041 atcgagatag cgttcgccgt tggtcgctat cagccaggcg ccttcgccgc gctggaagcc
     2101 gagatcgacc ctggcgaata cggggagcag atgcgacgtc gcgctgtcgg tcatgttgat
     2161 cactgaaggt ttggcgccgg ccccgacgcg cgcattggcg ccatcgcatc aacaaccagc
     2221 cctggaaaac aaaacgtgcc gccttttcgg ggcggcacgt gacaggcatt ctatgcgggt
     2281 ggcgatagct gtcaacctgc caatggaacc caatgggccc ggaatccatt ggtgcggaag
     2341 gtgtggacgc gttgcgtctg actcttgcgc gggagtcacg gcatattgta gccttcggat
     2401 tgtcgggtac gacatctcgt gcggcagtcg taatcctcat tcgtccagtg ttcggcgtaa
     2461 acgtccgggc gcgctttccg cgcccggcga cggttaaggg attctgcctg cagggttttt
     2521 gaacgaatgg agtcgcaccg cggcctcgag aatggccggc gcgatacgaa gggaaaattg
     2581 cgatgacggt attgacctgg accgacgatc gcgtcgagca actgaaaaaa ctctgggaga
     2641 gcgggctctc cgccagccag atcgccgcgg aactcggaaa tgtgacgcgt aatgccgtga
     2701 tcggcaaagt ccatcggctt ggcctttcgg gccgcgccaa gagcccctca tcggcggcac
     2761 cgcgtcagcg caaggcccgc ccggcccagc acatgatgcg ggttgcacgt ccggtctcgc
     2821 gcggcaatac cgcgctggcc cacgccttcg aagtcgagat ggaaccggat ccgatcgcct
     2881 tcgacaatgt ggtgccgata agccagcggc tgtcgctgct ggaactgagt gaggcgacct
     2941 gccactggcc gatcggcgat ccctccagtt cggaattctt cttctgcggc ggcaaggcgc
     3001 tgagcagcct gccctattgc gcacatcact cgcggatcgc ctatcagccg gcgagcgatc
     3061 gccgccgcca gccgccgaag ccgacaaggt agattgcgga cttcgtaccg aggtccgatc
     3121 tccaagtgcg attattactt cgtcgtccgg cgaacgccgg gacccataac caccagcttg
     3181 tgtggttacg tcaggtagtt tgctccacgt tttcgcaaaa tcgatacgtc acggcgtacg
     3241 ggtcccggcg ttcgccggga cgacggcgga tattatcttg ccgccttgct attccacttt
     3301 cgcgaaccgg tcatcgagcg cgtagccggc gccgcgcacg gtgcggatcg gatcctgctc
     3361 gcggcccggg ttgagcaatt tgcgcaaccg gccgatatgc acatccacgg tacgctcgtc
     3421 gatatagata tcgcggcccc acacgctgtc gagcagctgc tcgcggctga acacccgtcc
     3481 cggatgctcc agaaaaaatt ccagcaggcg atactcggtc gggccaaggt cgatcgggcg
     3541 gcccgagcgg gcgacccggc gcttttcgcg atcaagctcg atgtcgccat aggtaaggac
     3601 ggtggccagg cgctcggggc tagcgcggcg caacagaccc ttcacccgcg ccagcaattc
     3661 cggcaccgag aacggcttga cgatgtaatc gtcggcgccg gtcgccagcc ccctgacccg
     3721 ttcgctttcc tcgccgcgcg cggtcagcat gatgatcggc agcgaccgcg tctcggggcg
     3781 cgcccgcaag cgacggcaca gctcgatccc ggacaggccc ggcagcatcc agtcgagcac
     3841 gatcaggtct ggcacgcgct ccttcagacg agtgtcggcg tcgtcgccgc gcccgaccgt
     3901 ttcaacgtcg tagccttcgg cgtcgaggtt gtagcgcaac agcgtggtga gcgcttcttc
     3961 gtcttccacc accagaatgc gcgcgctcat ggcatagctc tctctttgtt tgaatggatt
     4021 ttcttcacgc aaatggctgt ccgccccgca tcaattgcgg ggcaggcttt cggttgaaag
     4081 cgctttctca attccccggt acggtcgtgg caaaggtcgt catatcgcct ttcgggcgct
     4141 tgtcgagtat ctgctggcct tcgatcatat agaacacggt ttccgcgata ttggtggcgt
     4201 gatcgccgat ccgctcgatg ttcttggcgc aaaacatcag atggatgcag aaactgatat
     4261 tgcgcggatc ttccatcatg taggtcagga gttcgcggaa cagcgaagta cagatggcgt
     4321 cgacttcctc gtcgcccttc cagaccgaca tcgccgccgg caaatcatgc gccgcatagg
     4381 cgtcgagcac ggatttgacc tgcgactgca cgaggtcggt catgtgctcg aggccgcgga
     4441 tcagcttcag cggatggaaa tcgttttcca gggccgcgac ccgcttgccc atgttcttgg
     4501 caagatcgcc gatccgttcg agatcggtgg cgacccgcat ggcgccgacg atctcgcgca
     4561 ggtctatcgc catcggctgg cgccgcgcga tggtcaacac cgcgcgttcc tcgatcacgc
     4621 gctgcaaatt gtcgatttcg acgtcgttgg taacgacacg cttgccgagt gcgatatcgc
     4681 ggcggaccag cgcatccacg gaatcgacga tcatgcgctc ggcaagaccg cccatctccg
     4741 cgaccagccg ggtcagttcc tgcagatcgc tgtcgaacgc cttggcggta tgttcagaag
     4801 ccatgtttct ctcctcagcc gaaccggccg gtgatgtagt cctgggtgcg tcgatcgctc
     4861 ggcgaggtga atatcttgtt ggtctcgtcg aactcgatca gttcaccgag atacatgaag
     4921 gcggtcttgt cggagacgcg cgcggcctgc tgcatgttat gggtgacgat ggcgatggtg
     4981 tagtcctccg ccagttcctg gatcaattcc tcgatcctgg cggtggagat cggatcgagc
     5041 gccgagcacg gctcgtcgaa caggatcacc tcgggccgca ccgccacggt gcgcgcgatg
     5101 cagagccgct gctgctggcc gccggacagg ctgaggccag aggcgttgag cttgtccttg
     5161 acctcgttcc acagcgcgcc gccgcgcagc gccttctcga cgcggccgtc catctcggat
     5221 ctggaaatct tttcgtagag ccggatgccg aacgcgatgt tctcatagat cgtcatcgga
     5281 aacggcgtcg gcttctggaa caccatgccg acccgcgcgc gcagcagatt gagatcaagc
     5341 ttgggatcga ggatgttggt ctggtcgagc atcagctgac cggtggcgcg ctggcccgga
     5401 tagagatcgt acatccggtt gaaaatccgc agcaacgtcg atttgccaca gccggacggc
     5461 ccgatgaagg ccgtgacgcg atgggtgccc agcgtgaggt tgatgttctt cagcgcaggg
     5521 tgttcgccgt aatagaaatt cagattgcgg gccgtaacct ttgccggcgc ctccgcatgg
     5581 cccgccattg acggcaccgg ggcgtttgga acactcacgg acacggaaag atcgttcatt
     5641 ttgcagtcct ctcggcgcca agaatgcgcg caccaatatt cagggcgagt acggtgagag
     5701 tgatgatcag ggctccgctc caggcgagtt gcttccagta ggcgtagggg ctctgcacga
     5761 agttgttgat ggtgaccggc aggttggcca tcgtcttggt gaggtcgagg ctgaagaact
     5821 gattgctgag cgcggtaaac agcagcggcg cggtctcgcc ggccacccgc gcggtcgcca
     5881 gcagcacgcc ggtgatcagg ccggcgcgcg ccgcgcggta ggcaatgcgc ttgatgacga
     5941 gcgaacgcgg caggccgagc gcagaggccg cctcacgcaa cggattgggc accagcagca
     6001 gcatgtcttc ggtggtacgg accaccacgg gaatgacgat cacggcgagc gccagacatc
     6061 cggcgaaagc cgagaacccg cccatcggca ccacgatggc gccgtagatg aacagaccga
     6121 tgatgatcga gggtgcgctc aacaggatat cgttgatgaa gcggatcacc gaggtgagcc
     6181 ggtcgttccg gccgtattcg gcgagatagg tgccggcgaa cagaccgagc ggcgcgccga
     6241 tgccgacgcc gatcacggtc atgatgaccg aaccggtgat cgcgttcagc aggccgcctt
     6301 cgctcgatcc cggcggcggg gtgttttggg tgaagatctg gacattcagc cccgcgatcc
     6361 cgttgaacag cagggtgaac aggatcagcg ccagccaggt cacgccgaac agcgcggctg
     6421 caaagcaaag cccgcggatg atgaggtcga cgcggcggcg gcgcgtgtaa atcgggttca
     6481 tgatcttcaa ttcccggcct tctgttccag ccgcatcagc atcaggcgtg cggcggccag
     6541 aacgaagaag gtcagcacga acagcaacag gccgagcagg atcaggccgg actggtgcaa
     6601 accgtcactt tcggcaaact cggatgcgat cgccgccgat atcgtggtgc ccggcgcgaa
     6661 gatcgaagac gagatgcgga acgaattgcc gatgatgaag gtgaccgcca tggtttcgcc
     6721 gagcgcgcgg ccaagcgcca gcatgatgcc gccgatgacg ccgacgcggg tgtaggggat
     6781 caccacattg cggaccactt cccaggtggt gcagccgacg ccgtaggctg cctccttgag
     6841 caccggcggc acggtcttga acacgtcgac cgagatcgcg gtgatgaacg gcagcaccat
     6901 gatcgcgagg atcagcgccg cgttgaacag gctgaggtag gacggcgggc ctccgaaaat
     6961 cgatccgaga acgggaacgc cgccgaacaa gccgatcatg aacggctgga acgtataggc
     7021 caggaacggc cccagcacga agaaccccca catgccgtag atgatcgaag gaatgcccgc
     7081 gagcaattcg atggcgatcc cgatcggacg gcgcagccat tgcggacaaa gctcggtcag
     7141 aaagatcgca ataccgagcc cgaccggtat cgcgatgagc atcgcgatga aggaggtcac
     7201 cagcgtgccg tacatgggac cgagcgcccc gagcaccggg ggatcggccg acggcgccca
     7261 gcgctgcgtc cacaggaacg cgaagccgta tgccttcatc gccggccatg cgccgacgat
     7321 cagcgagaga atgatgccgc cgaggatgag cagcaccgaa atagcgcaga tgcgggtgat
     7381 ccagtagaag gtgacgtcgc cgagcttgaa agcgttgagc gccttggcgc ggtcataagg
     7441 cccggcggct tccgctacat cgctctgaac ggccatgtct gccacgccaa tcccctgtac
     7501 tgctttactt gagtggtctc gttcccggac gctgcaccgc gtccgggaac gaaacttttc
     7561 ggctcagctc ttgatatcag ccgaccaggt cttttcgatc agcttgacca ccggctccgg
     7621 catcggaatg tagtcgagtt cctcggccat cttgtcgccc ttggtgaaag cccatttgaa
     7681 gaacttgacg gcctcggccg acgccgcctt atcaaccggc tccttgtgca tcaggatgaa
     7741 ggtcgcagcc gtgatcggcc acgacttctc gccgggctga tcggtgagga tcacgtaata
     7801 gccgggggcc ttcgcccagt cggcgttcga agcggcagcc tggaacgcct ccacggtcgg
     7861 ctgcacggtc ttgccggcct tgttgatcag agccgcgtag gtcagcttgt tctgcttggc
     7921 ataggcatac tcgacgtaac cgatcgcgtt cttggtttga ccgatattgc cggccacgcc
     7981 ttcgttaccc ttggcgccga cgcccgccgg ccattcgaca gcggttccgg agccaacctt
     8041 ggtcttccac tccgggttcg acttcgacag gtagtcggtg aaattgaagg tggttcccga
     8101 gccgtcggag cggcgcacca cggtgatggc gtcggtcggc agcttcagtt tcgcattgag
     8161 cttggcgatc gccggatcat cccacttggt gatcttgccg agatagatgt cacccagcac
     8221 ttcgccggac agcaccagtt cgcccggctt gatgccttcc agattcacga ccggcacgat
     8281 cgcgcccatc accatcggcc actgcaccag gccatccttc tcgagctgct cggccttcag
     8341 cggcgcgtcg gtggcgccga atgtcacggt cttggcctgg atctgcttga tgccggcacc
     8401 ggagccgatc gactggtagt tcaggccgtt gccggtctcc tttttgtagg cgtcggccca
     8461 cttcgaatag atcgggaacg ggaatgtcgc gcccgcgcct gtaatgtcgg cggcgaatgc
     8521 cgacgtcgaa gcggcaacaa ggccggccgc gacgatagct ttcacgaaat tcattgctgg
     8581 tctccatcgg tgagcgaagc gccggacgcg cccgattgcg ctcacgccgc ccatttaggt
     8641 gcggtcaatg ccgcttttac gaaggtttaa tgacagttgg atgacacggg caagcagttg
     8701 aaattgcttt gatttacacc gctgcgggcg atcgcagctg gggaaaacag gcggtgaagg
     8761 tggcgccgtt tttcggcacg ctttcgatca ggagccggcc gcggtgccgg ttaacgatat
     8821 gtttgaccag cgatagaccc aggcccgtgc cgccctgggc gcggctgtcg ccgacatcca
     8881 cccggtagaa ccgctcggtg agccgcggca gatgttccgg tgcgatgccg ggaccgaaat
     8941 cgcgcaccat gacgcgaatc tccggcaagc cctcggctga catcgccgct gtcagcgaga
     9001 caatgatctt gccgccggat gcgccgtatt tcagcgcgtt ctcgatcaga ttttcgaaca
     9061 gccgcagcaa ctcctcgcga tcgcccgcga tcacgacagg ccggtccggc agctggatgt
     9121 cgatcgcgac ctgacggtcg cccgccagcg gctccagccc gtccaccacc tgccggatga
     9181 tcgggacgat atccaccagc gtatcggggc ggacatgggc tgatagttcg acccgcgaca
     9241 gcgacaacaa atcgtcgatc agccgggcca tccgcgtcgc ctgcgtgtgc atgatgccga
     9301 gaaagcgttc gcgcgccttg acatcgtccc tggccggacc ctgcagggtg tcgataaaac
     9361 ccgacagcgc ggccaggggc gtgcgcaatt catgactggc attggctacg aagtcggcgc
     9421 gcatttcctc gacccggcgc agcggtgtct ggtcgtggaa tgtcatcagc atgcatttgt
     9481 cgatgccgcc gaacagcgtc ggcaccggta ccggggtgac gatcaattcc atccagcgat
     9541 cgaccggcac atggtcgaga taggtcgcgc gccggctttc cgtggtggcg atggcctcgc
     9601 gcagcgcggt aatgatttcg ggtgagcgca acgcgaactg ggcgagctcg ttcttgcgca
     9661 gcgccggcgc gagctgagcc gccgccgcat tgaggtggat gacgcggccg gcgcgatcga
     9721 gcagcaccgc gggatccggc atgccggcaa ccacggcgct gaccgcggcg gcttcgaccg
     9781 ggttgacgcc gcgcacatct tcacgcgagg cgaccgcgtc gtgcaaccgc cacggcacca
     9841 gcgcggccgc accgatgcag atgaaaaccg cggtggcgcg caccagcgac aattcgccga
     9901 aggcgacgag cacggcgagc gcgagaccgg cggcgagcag gataatggcc gaatggcgca
     9961 gccggtccgg ccacggcgag aagaaggagc cagaagaaga ttcatcaata gccatcggac
    10021 cggctctctt ccatcgggtc cgtatcgtca ggcgccggtg tccccacgct cacgcacgta
    10081 tacggcctca cgtttcggct cggcgtcgag ccccggcctc gcccggcgca ggcgcttcag
    10141 ccgagcgccc agtatgacct cccgaaacgt cagcaagatt acagatatga caaacggcgc
    10201 aagataatag aggacgcgga aaagaagcat ccccgcgagc agctcttccc ggtccatctg
    10261 ccaaaggccg accagcatg
//
LOCUS       DATNOI010000074         6018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2112695,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000074 DATNOI010000000
VERSION     DATNOI010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2112695"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="VN968_02515"
     CDS             complement(<1..1669)
                     /locus_tag="VN968_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL28155.1"
                     /translation="MNDMTPTREQREAKLEGMGCKRKRVEDARFTQGKGNYVDDLKLP
                     GMLHGDFVRSPHGHARVKSINSEEALKVPGVLAVITAETLKTVNLAWMPTLAGDVQMV
                     LADGKVLFQNQEVAFVVATDRYAADDGINKVVVEYEPLPVVIDPFKAMDADAPVLRED
                     LAGKTTGAHGPRKHHNHIFEWTVGDKDLTDAAFRKADVTIKEMLSYHRTHPSPLETCQ
                     CVCSFDKIKGELSIWGTFQAPHVIRTVVALIAKIPEHKIHVISPDVGGGFGNKVGAYP
                     GYICAAVASIVTGKPVKWVEDRIENLTATSFARDYHMTTEIAATKEGKVTGLRVHVLA
                     DHGAFDACADPSKWPAGFFNIVTGSYDFPVAHLSVDGIYTNKAPGGVAYRCSFRVTEA
                     AYCIERGMDILAQKLGMDPAELRLKNFIKPEQFPYHSALGWEYDSGDYHTAMKKMMET
                     VDYAGLRKEQADQRAAFKRGETREIMGLGVSFFTEIVGAGPSKNCDILGIAMFDSCEI
                     RLHPTGAGIARVGTKSTGQGHETTWAQIIATEIGIPADDIMVEEGNTD"
     gene            complement(1666..2166)
                     /locus_tag="VN968_02520"
     CDS             complement(1666..2166)
                     /locus_tag="VN968_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL28156.1"
                     /translation="MAKTHVTMKVNGAEVEGLVEPRTLLVHFIRENLLLTGTHIGCET
                     THCGACTVDLDGMSVKSCTMFAVQAQGSEITTIEGVANADGSLSALQEGFRMMHGLQC
                     GFCTPGMILRAQRLLQENPSPSEAEIRMGISGNICRCTGYQNIVKAIQYAAAKINGVQ
                     FQEAAE"
     gene            complement(2374..3237)
                     /locus_tag="VN968_02525"
     CDS             complement(2374..3237)
                     /locus_tag="VN968_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXL28157.1"
                     /translation="MIPGPFSYHRPATVADAVKLLSTLGEEARPLAGGHSLVPMMKLR
                     LATPEHLVDLHGIDGLKGIRRDGNRIVIGAMTTQHQLLASDEIGKSLPILHETALLIA
                     DPQVRYMGTIGGNVANGDPGNDMPALMMTLGASYRLEGPAGGRDIAASEFYQGAYFTS
                     LEPGEILTAISIPAPAAGHGYAYEKLKRKIGDYATAAAAVVLTMAGGKVASCAIGLTN
                     LHETPLLAVDAAKAVIGTSLDEATLKKAAAAAVAIMSPAADARGPIEYRKHVGGIMVT
                     RALTRAAGRAS"
     gene            complement(3379..4617)
                     /locus_tag="VN968_02530"
     CDS             complement(3379..4617)
                     /locus_tag="VN968_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HXL28158.1"
                     /translation="MFEGHDPYLVALSVVIAILGGYTGFSLAARIRGTPDVSHRVLLA
                     GAAAFLAVGIWTMHFVGMLAAPIPADTVYLVLPTIVSFLICVLVVGISLFFVSIGEPS
                     LRRVVSSAVLLGAGVASMHYVGIHGLAGHFAIAHDTPMILLSILIAVVTAYGGLRAFL
                     ARQDGVRLIVSSIAFGVAVAGMHYTAMDGMHFVPLADGSHHHIGGLAASQQILSVVVA
                     LLCFVIAAGFLLSLVPDPHRQAMAAATINPVAGAAVGVAQQALPEHGPAPLPRPMSAP
                     LGGLGQPPRAAPAPRLPVEGANGTHFINSADVRSVRADAHYTRVHDGTRERMCPWSIS
                     EAEEQLDPALFLRVHRSHIVAIPHVTFVRKEGDGAIVELDGPSPHRVPVSRAKIAEVK
                     ARLGLAKRHAQSAAGLGREA"
     gene            complement(4719..5309)
                     /locus_tag="VN968_02535"
     CDS             complement(4719..5309)
                     /locus_tag="VN968_02535"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HXL28159.1"
                     /translation="MNQMLQEPHLQDAPQVEDSPEANESSEKIGRPPVPAHPRFAKPQ
                     RVAFVQSSWHREVVEECRIAFLDEIEARHIARAQIDLFEVPGSFEIPLHAQLLAKTRR
                     YTAIVAAGLVVDGGIYRHEFVADTVIKALMDVQLRTEVPVFSAVLTPQQFHESAAHFD
                     FFRKHFATKGVEVAEACANTLLSLERLRGQVAAGIV"
     regulatory      complement(5378..5539)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
BASE COUNT         1100 a         1904 c         1902 g         1112 t
ORIGIN      
        1 tgtcggtgtt gccctcctcg accatgatat cgtcggcggg aatgccgatc tcggtggcga
       61 tgatctgcgc ccaggtggtc tcatggccct gcccggtgct tttcgtgccg acccgggcaa
      121 tgcccgcgcc ggtcggatgc aagcgaattt cgcaggaatc gaacatcgcg atgcccagga
      181 tatcgcagtt cttcgacgga ccggcgccga cgatctcggt gaagaacgac acaccgaggc
      241 ccatgatctc gcgggtctcg ccgcgcttga acgccgcgcg ctggtccgcc tgctccttgc
      301 gcaggccggc atagtcgacc gtttccatca tcttcttcat cgcggtgtgg tagtcgccgg
      361 aatcgtactc ccagcccaac gcggagtgat aaggaaactg ttccggcttg atgaaattct
      421 tcagccgcaa ttccgcagga tccatcccga gtttctgagc gagaatgtcc atgccgcgct
      481 cgatgcagta ggccgcctcc gtcacccgga acgagcagcg ataggcgaca ccgcccggcg
      541 ccttgttggt gtagatgccg tccaccgaca gatgcgcaac cgggaagtcg taggagccgg
      601 tgacgatgtt gaagaagccg gccggccatt tcgacggatc ggcgcaggca tcgaacgcgc
      661 cgtgatcggc gagcacgtgg acgcgcaggc cggtgacctt gccttccttg gtcgccgcga
      721 tctcggtggt catgtgatag tcacgcgcaa acgaagtggc ggtgaggttc tcgatgcggt
      781 cctcgaccca cttcaccggc ttgccggtga cgatcgaagc caccgcggca cagatgtagc
      841 cgggataggc gcccaccttg ttaccaaaac cgccgccgac gtcgggcgag atcacgtgga
      901 tcttgtgttc ggggatcttg gcgatcagcg ccaccacggt gcggatcacg tgcggcgcct
      961 ggaaggtgcc ccagatcgac agctcgccct tgatcttgtc gaacgagcac acgcactggc
     1021 aggtctccag cggcgacgga tgggtgcgat gataggagag catctccttg atcgtcacat
     1081 ccgccttcct gaacgcggcg tcggtgagat ccttgtcgcc gacggtccat tcgaagatgt
     1141 ggttgtgatg cttgcgcggc ccatgcgcgc cggtggtctt gccggcgaga tcctcgcgca
     1201 gcaccggcgc atcggcgtcc atggccttga acggatcgat cacgaccggg agcggttcgt
     1261 attcgacgac caccttgttg atgccgtcat cggccgcata acggtcggtg gcgaccacga
     1321 aggcgacttc ctgattctgg aacagcacct tgccgtcggc cagcaccatc tgcacgtcgc
     1381 cggcgagcgt cggcatccag gcgaggttga cggttttgag agtctcggcg gtgatgacag
     1441 ccaatacgcc cggcaccttc agcgcttctt cgctgttgat cgacttgacc cgtgcatgcc
     1501 catgtggcga tctgacgaaa tcgccgtgca gcatgcccgg cagtttcaaa tcgtcgacat
     1561 aattgccctt gccttgcgtg aagcgggcat cctcgacccg cttgcgcttg cagcccatgc
     1621 cttcgagctt ggcttcacgt tgttcccgcg tgggagtcat gtcgttcatt ccgcagcctc
     1681 ctggaattgg acgccattga tcttggcggc ggcgtactgg atcgccttga cgatgttctg
     1741 atagccggtg cagcggcaga tattgccgga aatgcccatc cggatttccg cctctgacgg
     1801 cgacggattc tcctgcagca gacgctgcgc gcgcaagatc atgcccggcg tgcagaaacc
     1861 gcattgcagc ccgtgcatca tgcgaaagcc ttcctgcaat gccgacagcg aaccgtcggc
     1921 attggccacg ccctcgatgg tggtgatctc ggagccctgg gcctgcaccg cgaacatcgt
     1981 gcagcttttc accgacatgc cgtcgagatc gacggtgcag gcgccgcaat gggtggtctc
     2041 gcagccgata tgggtgccgg tgagcagcag attctcgcgg atgaaatgca ccagcagcgt
     2101 gcgcggttcg acgaggcctt cgacctcggc gccgttcacc ttcatggtca catgggtctt
     2161 tgccattttt gtctctccct agtaccgctt gtccgaagtt cgcaacatat ctcggacctc
     2221 atcctgagga gcatcgcgaa ggcgatgcgt ctcgaaggat gactccgggt gacgacagcg
     2281 ccagccgcca tccttcgaga cgcgcgcgaa aggcgcgctc ctcaggacga ggtccgcgac
     2341 tgttgctgcc gacggtttca tgcaatcgat cccttagctg gcgcggccgg cggcgcgggt
     2401 cagcgcccgc gtcaccatga tgccgccgac atgtttgcgg tattcgatcg ggccgcgggc
     2461 gtcggcggcc ggcgacatga tcgccaccgc cgccgccgcg gcctttttga gcgtcgcctc
     2521 gtcgaggctg gttccgatca cggccttcgc cgcgtccaca gccagcagcg gcgtttcgtg
     2581 cagattggtg aggccgatcg cgcaactggc gaccttgccg cccgccatcg tcagcacgac
     2641 cgccgccgca gcggtggcgt agtcgccgat cttgcgcttg agtttttcgt aggcgtagcc
     2701 gtgaccggcc gcaggcgccg gaattgaaat cgcggtcagg atctcgccgg gctccagcga
     2761 cgtgaaatag gcgccctgat agaattccga agccgcgatg tcgcgcccgc ccgcggggcc
     2821 ttcgagccgg tagctggcgc ccagcgtcat catcagcgcc ggcatgtcat tgccgggatc
     2881 gccattggcg acgttgccgc cgatcgtgcc catgtaacgc acctgcggat cggcgatcaa
     2941 aagcgcggtt tcgtgcagga tcggcagcga tttgccgatc tcgtcggacg ccagcagctg
     3001 gtgctgggtg gtcatcgcgc cgatcacgat gcgattgccg tcgcggcgga tgcccttcaa
     3061 cccgtcgatc ccgtgcagat cgaccagatg ctcgggggtg gccagcctaa gcttcatcat
     3121 cgggaccaga ctatggccgc cggcaagggg gcgggcttct tcacccagtg ttgacagtaa
     3181 tttgaccgca tcggccacgg tcgcgggccg gtgatagctg aatgggccag gaatcatggt
     3241 ctcctccatc cgtcctgtga gtgcagacgt tggatgagag gctgttcccg gaatttttcg
     3301 taagcaaggc cacggcggtc gacggggcac ggactaagga aattcgcacg aattgcggat
     3361 tacggcgcac gaaatgcgtc aggcttcgcg acccaatccg gctgctgact gcgcgtgacg
     3421 cttggcgaga ccgagccggg ccttgacctc ggcgatcttg gcgcggctca cgggcacccg
     3481 gtgcggcgat ggcccatcga gctcgacgat ggcgccgtca ccctccttgc gaacaaaggt
     3541 aacatgggga atcgccacaa tgtggctgcg gtgcacccgg aggaacagcg cgggatcgag
     3601 ctgctcttcg gcctccgaga tcgaccatgg gcacatccgc tcgcgggtgc cgtcatgaac
     3661 cctggtataa tgggcgtcgg cccgcacgct ccgcacatcg gcgctattga tgaaatgggt
     3721 gccgttggcg ccttcgaccg gaagccgggg cgccggggcg gcgcgcggcg gctggccgag
     3781 gccgccgagc ggcgccgaca tcggccgcgg caggggtgca ggaccgtgtt cgggcaatgc
     3841 ctgctgcgcg acaccgaccg cggcccccgc aaccggattg atcgtcgccg ccgccatcgc
     3901 ctgccggtgc ggatccggca ccagcgagag caggaagccg gccgcaatga caaaacagag
     3961 cagcgccacc accaccgaga ggatttgctg cgacgcggcc agcccaccga tatggtggtg
     4021 cgacccatcc gccagcggaa caaaatgcat gccgtccatc gcggtgtagt gcatgccggc
     4081 caccgcaacg ccgaacgcga tcgagctcac gatcagccgg acaccgtcct gccgagccag
     4141 gaatgcgcgc aggccgccat aggcggtgac gacggcaatc aggatggaga gcaggatcat
     4201 cggggtgtca tgcgcgatgg cgaaatggcc ggcgagaccg tgaatgccga cataatgcat
     4261 gctggcaacg cccgccccga gcagcaccgc cgacgacacc acgcggcgca gcgaaggttc
     4321 gccgatgctg acgaaaaaca gggatatgcc gaccaccagt acgcagatca ggaacgacac
     4381 gatggtgggg agaacgagat agacggtatc agccggaatc ggcgcggcca gcatgccgac
     4441 aaaatgcatg gtccagatgc cgaccgcgag gaacgcggca gcgccggcca gcagcacgcg
     4501 atggctgacg tccggcgtcc cgcggatacg cgccgcgagg ctgaagccgg tgtacccgcc
     4561 caggatcgcg atcaccaccg agagcgcgac gagataggga tcgtgtcctt cgaacatgat
     4621 cgtctcggcc gccagtctcc acgggcgtgc ctcctcccgc cgccgacttt ataggcgcgg
     4681 cggcggaaat gacaatcaat ccgtccgggc tcgctttcct aaacgatccc cgccgcgacc
     4741 tgaccgcgca gccgctccag actgagcagt gtgttggcgc aggcttccgc gacctcgacg
     4801 cccttggtcg caaaatgctt gcggaagaag tcgaaatgcg cggcgctctc gtggaactgt
     4861 tgcggcgtca gcaccgcgga gaataccggc acctcggtgc gcaactgcac gtccatcagc
     4921 gccttgatca cggtatcggc gacaaattca tggcggtaga tgccgccatc gaccacgagc
     4981 cccgccgcga cgatcgcggt gtagcgcctg gttttggcga gcagctgcgc atgcagcggg
     5041 atttcgaacg aacccggcac ctcgaacaga tcgatctgcg cgcgcgcgat gtggcgcgcc
     5101 tcgatctcat cgaggaaggc gatccggcat tcctccacca cctcgcgatg ccaggacgac
     5161 tgcacgaagg cgacccgctg cggttttgca aatcgcgggt gcgcgggaac cggcggccga
     5221 ccgatctttt cggaagattc gtttgcttcg ggagagtctt ctacttgggg agcgtcttgt
     5281 aagtggggtt cttgcaacat ctgattcatg gctttcctct ttcaggacca gaatcagggc
     5341 atacggaacg acacgccgcg cgcaatgcgg ctgtcgcgac cgttctcttt catccggact
     5401 ttaaccgtcg gcttcggaat cacaccgaat ctgctgaccc tttttcccaa ggggattcca
     5461 agggaagaag gcgctcgcgg gcttgggctt atccaccctt accgccggtg gggactttca
     5521 ccccgccctg agaacatcgg ccgcccggta tgagcgacct gaggggaact atgaccaatg
     5581 gccgcgacgc cagcaagcac cttcatcatg ggaaatctgc atgtccctat gccgagcgcg
     5641 tattccgcaa aagtgggtgc cggttttgcg catagaatac gcgcaaatta tagattagga
     5701 gcattttctt gccggctaac cggattccgc ttagctggca agaaaatgct ccgtggcgaa
     5761 tctaaggcga tttgcttccg cgaaattaac gattaaggcg ggccagtccg gctcgcttgg
     5821 agcgaattcc gcgcgccgga ctcggcccgc gcgaatccga ctccggtttg ttctcaaaat
     5881 taataatttc ggcgcgcgat cagctgtgga cgagcgccgc tttggctgtg gacggactcc
     5941 ccctgcagtt gcgccgattc cgcaaatcac atcacttgat cccggcaggc ccccggcgcg
     6001 atcaggggat tgcagtcg
//
LOCUS       DATNOI010000075        10974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_678811,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000075 DATNOI010000000
VERSION     DATNOI010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10974
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678811"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(527..1351)
                     /gene="map"
                     /locus_tag="VN968_02540"
     CDS             complement(527..1351)
                     /gene="map"
                     /locus_tag="VN968_02540"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXL28160.1"
                     /translation="MSYIEASDTSLRKTGQIKLHGPSGFAGMRKAGALVAKCLDELTD
                     IVKPGAPTSRIDDFVREFAFGHGAYPATLMYRGYRFSTCTSINHVVCHGMPGDRLLKE
                     GDIVNIDVTFIVEGWYGDSSRMYAVGPVARKAERLIEVTYEAMMRGIAAVKPGATTGD
                     IGHAIQSFVEPQGMSVVRDFCGHGLGRMFHDEPNIIHIGRPGEGVTLKPGMFFTIEPM
                     INLGKPHVKILSDGWTAVTRDRSLSAQFEHSVGVTANGVEIFTLSERHAEKPPAAD"
     gene            complement(1494..2807)
                     /locus_tag="VN968_02545"
     CDS             complement(1494..2807)
                     /locus_tag="VN968_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HXL28161.1"
                     /translation="MTMDLNEIAELLHSAARSVGAEVTSPWFYLQVGLILIGAGIAIG
                     AGVAIRARVDTSSLAMGWPAPLRLFMRVLVGSASTAVFALLMSLARLVMLSSTWPSRS
                     YLLSVAASLALAWLVIRLVTSVIRNPFIVRLVSLSAWLVAALSILGLLEPAIEALDSV
                     SIVLGGLRLTPLLLIKLGVLLILALWVTNLATNFVESRITRSTDLTPSVQVLLIKVIR
                     LALLFFAVAVVLSAVGINLSALAIFSGAAGVGIGFGLQKIVANFISGIILLADKSVKP
                     GDLITIGDSTGRINEMKTRYISVAAGDGREFLIPNEDLVTQKVVNWTYTDKNTLVKVN
                     FGTNYEADPRLVCKLALDIAAAAPRAIKNKAPNCILVEFAEAGMKFSLTFWIADPDGL
                     DNVKSDAMLALWDAFKREGIHVPYPVREIRVRGALPVETIAETSK"
     gene            3027..4820
                     /locus_tag="VN968_02550"
     CDS             3027..4820
                     /locus_tag="VN968_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HXL28162.1"
                     /translation="MASLDSVSIAILLGAVLVMAGILSSLLALRFGAPLLLVFLVIGM
                     LAGDSGPGQLRFDDVRTTYLVGSVALALILFDGGLRTRFQNIRAVLAPSMVLATIGVL
                     LTALVTAPVAKYVLDLNWTEALLVGAVVASTDAAAVFLLVHAQGLRLRPRVGATLEAE
                     SGTNDPFAVFLTLMLVELISSGQSSVWHVVLEFGREAVLGAIVGVIGGRLVVLALNRV
                     ALPQGLHAPFVCTAALVIFGVAQIAHASGFLAVYLAGIIIGNRPTRAHNSVVTFLDAA
                     TWLAQIVMFVMLGLLVSPQRLAGSVIPAVVVALVLMLAARPVAVFLCLAPFRFNWREK
                     LFMAWTGLRGAVAIFLASIPMLVGLSKAYLYFDVAFVVVIISLLLQGWTLAVAARRLH
                     VALPRSDRGPRRVELDLPGQLEQELVGYSVRPKSLYFRRGLVPSWSKPTLVIRDQRIL
                     SPAEADPIQPGDYIYLLAPPEKAATLDRFFVDMLPSTEPDPHLLGDFTVSGEVTLGDI
                     AAAYGVSVDPEQAKLTLADYFDVHLDHAPKVGATLPLDSIVLVARSISGGRVNVVGLR
                     LPEDEDEAVAATRLAKLKRKLSEIWSSVAGI"
     gene            complement(4858..5325)
                     /locus_tag="VN968_02555"
     CDS             complement(4858..5325)
                     /locus_tag="VN968_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28163.1"
                     /translation="MALVIFFIPEPRKGVAEMVRVVSKGGTVAAYAWDMPGGGFPLQP
                     IQSEMFAMGFPPLRPPSAQVLIAEALQNLWTEAGLQGVETRKIEVQRTFNDFDDVWTT
                     AMLAPSTGPTVASMPPDDVELLKKLVRARLPADTASRITYSAWANAVRGNVPG"
     gene            complement(5325..5648)
                     /locus_tag="VN968_02560"
     CDS             complement(5325..5648)
                     /locus_tag="VN968_02560"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXL28164.1"
                     /translation="MAEPQIRFDDGAAYERMMGVWSRLAGEIFLDWLAPRSGLRWIDV
                     GCGNGAFTELIVERHAPAEVQGIDPSDGQLAHARTRPATRSAQFRQGDAMELPFPSRR
                     FDVAV"
     gene            complement(5726..6232)
                     /locus_tag="VN968_02565"
     CDS             complement(5726..6232)
                     /locus_tag="VN968_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28165.1"
                     /translation="MDASVITALAALAGAAIGGLTSVFASWLNQRAQARAQWVAQDKL
                     RRQELYKEFIGAASKCYIDALQHENADIPALVELYVRLGRMRILSSPNVIESAERVAR
                     RIVDSYLAPNKSFLELREMVNSGTIPTFFAISARPAARSSTRCAINSEESFLTAIKAR
                     ADRAPAGP"
     gene            complement(6240..7424)
                     /locus_tag="VN968_02570"
     CDS             complement(6240..7424)
                     /locus_tag="VN968_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007820841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL28166.1"
                     /translation="MSGLLHAFRNELRRIFTVKPAFSVLILGAAFYALFYPQPYLNEA
                     LRNVPIAVVDRDGTQSSRDFARLVDATPDVAITEVLPDLASAEREVFARRVDGILVIP
                     QYFERDLMHGRPSPVALYADASYFLIYQRVAGAVGAVARTVGAEVEAARLIAIGVDPA
                     IAVVAPDPMPLTAVPIFNPEAGYATYVLPAAFVLILQQMLLMGVGLLGTLPGADFAGD
                     DARRRRLSPVTLMAGKLLAYLALEAVILPIYLIVLPYLYGLPRLGVIVPILIFAVPFV
                     LSVSGLGFVVAGILRRPIRVALILAALGLPLFMIAGFSWPSEAIPPFIRLISFLVPST
                     SAIDGFVKLSQLGAPLAAAKSELFTLCALAVFYNLIGLWQMVRNTEPVNPMVSENASP
                     AS"
     gene            complement(7421..8644)
                     /locus_tag="VN968_02575"
     CDS             complement(7421..8644)
                     /locus_tag="VN968_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL28167.1"
                     /translation="MLARSGIRPGFRLVFWREVGWLWRRPFLLGLATIVPLTQMAFLA
                     AVFSAGLATRLPIGVLDLDSSDLSRTIIRMVDATPDSAVAVRVGDLAEGRSLILSGKI
                     HGLLMLPLYLQRDVFAGRRPEVVFFYNTQTLTTGNLTARGVNAAVPTAAAGIRLTLRS
                     AQGEPLDLAEADLQPIPVQTHALFNPTLNYVFFLLTALIPSVLQVVMVTTSAYSVGLD
                     VETPYRLRILRSLGGGLWPAMAGKILPYTILFLLVLGLSDTLLFVVLGLPLRGHPWLL
                     IMTGVLFILSCQLLGALLAVLLKPTLSAVSIATLVTAPAFGFMGIGFPRLGMNAFAYA
                     WGELLPGTWYLTARIDQTIRGTPVDLSFKPLFVLAAFAVGLAGLVAWKLESMRRSTHR
                     IEDRNAPQPLAKVPS"
     gene            complement(8646..9629)
                     /locus_tag="VN968_02580"
     CDS             complement(8646..9629)
                     /locus_tag="VN968_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXL28168.1"
                     /translation="MGKRSVRWLIGVVVVAAFATMLWLATRPPPLIIQGEVSADRVDI
                     SPRVAARIVTLKADVGDTMERGAVMAELESPQLVAARLAAQAALAVAKADLDRINSTR
                     PETIAARQADLNAAKADETLAQETYNRQIELARTGNAPQARVDEVTRNLDTATRKRES
                     AEAALQLATTGASKEERALAAAQVKQAEATLNQRDVDVAEFTIRSPITGQITARVAAL
                     GENFSAGAPLFSMIDLQNLWFTFNLREDLLGGLKIGDKFDLTVPALKSQVIPVRVTMI
                     NVQGQYAAWRATRATGDFDLRTFEVRALPIQPVDGLRPGMSAIVSWANRGR"
     gene            complement(9774..10448)
                     /gene="modC"
                     /locus_tag="VN968_02585"
     CDS             complement(9774..10448)
                     /gene="modC"
                     /locus_tag="VN968_02585"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HXL28169.1"
                     /translation="MLRIEVAKQLGEFSLQASLVSEGRVTGLFGASGAGKTSLVNIIA
                     GLLRPDRGSIVIDDETLDDTAAGVHVPAHRRRIGYVFQDARLFPHLDVRQNLDYGRRM
                     NRLADDHAERARVTALLDIGGLLDRRPGQLSGGERQRVALGRALLAKPRLLLLDEPLG
                     SLDEERKIEILPYLVRLRDEAGVPMVYVSHDAGEMRQLATSIVMLQRGRVIAFGGAEV
                     LPPAPT"
     gene            complement(10451..>10974)
                     /gene="modB"
                     /locus_tag="VN968_02590"
     CDS             complement(10451..>10974)
                     /gene="modB"
                     /locus_tag="VN968_02590"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HXL28170.1"
                     /translation="PPVVTGYLLLLTFGRRGLVGAWLADHLGIVFAFRWTGAALACGI
                     MSFPLLVRPIRLSIEAVDLRLEQAAATLGAAPWRVFLTVTLPLALPGVLAGMVLGFAK
                     AIGEFGATITFVSNIPGETQTISSAIYSLIQTPDGDAAALRLVAVSIVIAMSAMIASE
                     WFARRALLRLHGN"
BASE COUNT         2071 a         3419 c         3538 g         1946 t
ORIGIN      
        1 gagcgcgaag taagccgtaa aaccattgcg cggggaatgc cgggtcgttc cggtgtgacc
       61 gtggtgacta tgctcgtatg cttttttcat tgcatgcgag gccgcgggcg cattgcgcgc
      121 ccggcattcc ctgcgccctc tgattgcaga gagcgggacg tgaccagcaa acctcgcgcg
      181 aaaacgtgcg gcgagatcgc gtagtcatgt ctgcggatga ggctgtttga aattcgaatc
      241 cgtacggtcg tcattgcgag gagcgcaagc gacgaagcaa tccaacttct tgagtcgcgg
      301 cgatatggat tgcttcgcgg agcctgtcat cgggcgcgca ttcgcgcgac ccgttggctc
      361 gcaatgacgg atgcgtggcc gccacattcc cagccgtcat cgtccgcgaa gcggacgatc
      421 cagtattcca gagcagttgc gatgaaatcg aaaggccgcg gcgtactgga tcgtccggtc
      481 aagccggacg acgacagttg cgtgagaagc gacgaagcga ccgaggctag tccgcggccg
      541 gcggcttttc ggcgtggcgc tccgatagcg tgaagatttc gacgccattg gcggtgacgc
      601 cgaccgaatg ctcgaactgc gccgacagcg agcggtcgcg tgtcaccgcg gtccagccgt
      661 cggagaggat tttgacatgc ggcttgccga gattgatcat cggctcgatg gtgaagaaca
      721 tgccgggctt cagcgtcacg ccttcgccgg gccggccgat atgaatgatg ttcggctcgt
      781 catggaacat gcggcccagg ccgtggccgc agaaatcgcg caccacgctc atgccctgcg
      841 gctcgacaaa actctggatg gcgtggccga tgtcgccggt ggtggcgccg ggctttaccg
      901 cggcgatgcc gcgcatcatc gcctcgtagg tcacctcgat cagccgctcg gccttgcggg
      961 cgaccgggcc caccgcatac atccggctgg aatcgccata ccaaccctcg acgatgaagg
     1021 tgacgtcgat gttgacgatg tcgccttcct tgagcagccg gtccccgggc atgccgtggc
     1081 agaccacgtg gttgatcgag gtgcaggtcg aaaagcgata gccgcggtac atcagcgtcg
     1141 ccgggtaggc gccatggccg aaggcgaact cgcggacgaa atcgtcgatc cgcgaggtcg
     1201 gcgcccccgg cttgacgata tcggtcagtt cgtcgaggca ttttgcgacc agcgcgccgg
     1261 ccttgcgcat gccggcgaac ccactcggcc catgcagctt gatctgcccg gtcttgcgta
     1321 aggaggtatc ggaggcttcg atatagctca tgggctgatc atgttctggc gggcagaggg
     1381 ctgcgatggc ggatttttca ggctctaatt taatgatcgg agctgctagt gcaagccgag
     1441 gttgggccgc gagtttgtca tggctcggct ggttctgccc cagcaagatg gcatcatttc
     1501 gaagtttctg cgatggtctc gaccggcagc gcgccgcgga cgcggatttc gcgcaccgga
     1561 tagggcacgt ggataccctc gcgcttgaag gcatcccaga gcgccagcat cgcgtcgctc
     1621 ttcacattgt ccagcccgtc ggggtcggcg atccagaagg tcagggaaaa cttcatcccg
     1681 gcctcggcga attccacaag gatgcagttc ggagctttgt ttttgatggc gcgcggcgcg
     1741 gccgcggcaa tgtcgagcgc cagtttgcag accagccgcg gatcggcctc gtaattggtg
     1801 ccgaaattca ccttcaccag cgtgttcttg tcggtgtagg tccagttgac gaccttctgg
     1861 gtgaccagat cctcgttggg aatcaggaat tcgcggccgt cgccggcggc gaccgaaata
     1921 tagcgggtct tcatctcgtt gatgcggccg gtactgtcgc cgatggtgat gagatcgccg
     1981 ggtttgaccg atttgtccgc cagcaggatg atgccgctga tgaaattggc cacgatcttc
     2041 tgcaggccga agccgatgcc gacaccggcg gcacccgaga agatcgccag cgccgagagg
     2101 ttgatgccga ctgcgctcaa tacgaccgca accgcaaaga acagcagcgc aagccggatc
     2161 accttgatca gcagcacctg gaccgagggc gtcagatcgg tcgagcgggt gatccggctt
     2221 tccacgaagt tggtggcgag attggtcacc cacagcgcga ggatcagcag cacgccgagc
     2281 ttgatcagga ggagcggcgt cagccgcagg ccgccgagca cgatcgagac cgagtcgagc
     2341 gcctcgatgg ccggctcgag caggccgaga atgctcaacg ccgccaccag ccaggccgaa
     2401 agcgacacca ggcggacgat gaaggggttg cggatgaccg atgtgacgag gcggatgacc
     2461 agccacgcca aggcaaggct ggcggcgacc gacagcagat agctgcggct gggccaggtc
     2521 gaactcagca tcaccaggcg cgccaacgac atcagcagcg cgaacaccgc cgtcgaggcg
     2581 ctgccgacca gcacgcgcat gaacagccgc agcggcgccg gccaccccat cgcgagcgag
     2641 ctggtgtcga cccgcgcgcg aatggcaacg ccagcgccga tcgcgatccc ggcgccgatc
     2701 aggatcagcc caacttgcag atagaaccag ggcgacgtga cctcggcgcc gaccgaacgc
     2761 gcggcggagt gcaggagttc ggcgatctcg ttcaggtcca tcgtcaaaat ctcgtcgcaa
     2821 gccggatcat cggcaaactt gattcgaata gcctgcagat tctcataacc gcaagcccgg
     2881 cgccatcgct acggcacgct agtgccgtgg gttgagcccg gaaaaccggg cacattgccg
     2941 ctggtcgaag aaatgggggt tggcgtcgaa gtgtggcgac gataaggatg caattgcaag
     3001 catttgcgct tgtttggcgg aagctcatgg cgtcactcga ttcggtcagc atagccattc
     3061 ttctgggcgc ggttctggtg atggcgggca ttctgtccag cctgctggcg ctgcgcttcg
     3121 gcgcgccgtt gctgttggtg tttctcgtga tcggcatgct ggccggcgat tccggtcccg
     3181 gccagctccg cttcgacgac gtgcgcacca cctatctggt gggatcggtg gcgctggctc
     3241 tgatactgtt cgacggcggc ttgagaacgc ggtttcagaa cattcgcgcc gtgctggcgc
     3301 catcgatggt gttggcgacg atcggtgtgc tgctgaccgc gctggtcacg gcgccggtcg
     3361 ccaaatatgt gctggacctg aactggaccg aggcgttgct ggtgggcgca gtggtggcct
     3421 cgaccgacgc cgcggcggtg tttctgctgg tgcatgcgca gggcttgcgg ctgcggccgc
     3481 gcgtcggcgc gacgctggag gcggaatccg gcaccaacga tccgttcgcg gtgtttctga
     3541 cgctgatgct ggtcgaactg atttcgagcg gccaaagctc ggtgtggcat gttgtgctgg
     3601 aattcggccg cgaggccgtg ctcggcgcca tcgtcggcgt gatcggcggg cgtctggtgg
     3661 tgctggcgct caatcgcgtc gcgctgccgc agggcctgca cgcgcccttt gtctgcaccg
     3721 cggcgctggt tattttcggc gtggcgcaga tcgcgcacgc ctcgggcttc ctcgccgtct
     3781 atctcgccgg catcatcatc ggcaaccggc cgacgcgtgc gcataattcg gtggtgacgt
     3841 ttctcgacgc cgccacctgg ctggcgcaga tcgtgatgtt cgtcatgctc ggtcttttgg
     3901 tttcgccgca acggctggcc ggcagcgtta ttccggcggt ggtcgtggcg ctggtactga
     3961 tgctggcggc gcggccggtc gcggtgtttc tctgtctggc gccatttcgc ttcaactggc
     4021 gcgagaagct cttcatggcc tggaccggcc tgcgcggcgc ggtcgcgatc ttcctggcgt
     4081 cgatcccgat gctggttgga ttgtcgaagg cgtacttgta tttcgacgtt gcctttgtcg
     4141 tcgtcatcat ctcgctattg ttgcagggct ggacgctggc cgttgccgca cggcggctcc
     4201 acgtggcgct gccacgctcg gatcgcgggc cgcgccgcgt tgagcttgat ctgcccggcc
     4261 agctcgagca ggaactggtc ggctactccg ttcgcccgaa gagcctgtat ttccgacgtg
     4321 ggctggttcc atcctggtcg aagccgacgc tggtgatccg cgaccagcgc attctctcgc
     4381 cggcggaagc cgatccgatc cagccgggcg actatatcta tctgctggcg ccgccggaaa
     4441 aggccgcgac gctggatcgg ttttttgtcg acatgctgcc gagcaccgag cccgacccgc
     4501 atctgctcgg cgattttacc gtgtccggcg aagttacgct tggcgacatc gccgcggcct
     4561 atggcgtctc tgtggatccg gagcaggcaa aactgacgct ggccgactat tttgacgttc
     4621 acctcgatca tgcgccgaag gtaggcgcga cgcttccgct cgactccatc gtgctggtgg
     4681 cgcgcagcat ctccggcggc agggtcaatg tcgtcggact tcgcctgccg gaagacgaag
     4741 atgaagcggt ggcggcgact cggctcgcta agctcaagcg caagctgtcg gagatatggt
     4801 cgtcggtggc tgggatttag tcgtggtgac aagcggtggc tagggttcct cagttgttca
     4861 acctggcacg ttccccctaa ctgcgttagc ccatgcactg taggtgatac ggcttgcggt
     4921 gtccgccggc aggcgcgcgc ggacgagctt tttgagcagc tccacgtcat ccggtggcat
     4981 cgaagcgacg gtgggtccgg tactaggcgc cagcatggcg gtcgtccaaa cgtcgtcgaa
     5041 atcgttgaat gttctttgca cctcgatctt ccgcgtctcc acgccctgga gaccagcctc
     5101 ggtccataag ttttgaagcg cctcggcgat taaaacctgc gcgctgggcg ggcggagcgg
     5161 gggaaagccc atcgcgaaca tctcggactg gatcggctgc aagggaaatc caccgcctgg
     5221 catgtcccag gcataggcgg ccaccgttcc gcctttcgag accacccgca ccatctcggc
     5281 gacgcccttg cgaggctcgg gaatgaagaa gatcaccagc gccatcacac cgccacatcg
     5341 aacctgcgag aggggaacgg cagctccatc gcatcgcctt gccggaactg cgcgctgcgt
     5401 gtggcaggtc gtgtccgcgc atgggccagt tgcccgtccg atggatcgat cccctgaacc
     5461 tcggcgggcg cgtgccgttc aacaatcagc tcggtgaagg ccccgttgcc gcagccgacg
     5521 tcgatccatc gcagaccgga gcgcggcgcg agccagtcaa gaaagatttc gcccgccagc
     5581 ctgctccaca ctcccatcat ccgctcatac gcggccccgt cgtcaaagcg gatctgcggt
     5641 tcggccatcg gctatgttct ccgtttcaag gcatgccccg gatcagcgag aggttgtcgc
     5701 ggatttcctg aaggaccgca atctctcaag gtcccgctgg cgcacgatcc gcccgcgcct
     5761 tgatagccgt caagaagctc tcttcagaat tgatcgcgca gcgagtcgaa ctccgcgcgg
     5821 caggtctcgc tgaaatcgcg aagaaggtcg ggatcgtccc gctgttgacc atctcccgca
     5881 gttcaaggaa cgacttgttc ggtgccagat atgaatcgac gatcctccgc gccacccgct
     5941 cggcactttc gatcacattt ggcgaggaca ggattcgcat cctgcctagc ctgacgtaaa
     6001 gctcgaccag tgccggtatg tcggcattct catgctggag cgcatcgata tagcatttcg
     6061 aggcggcccc gatgaattcc ttgtagagtt cttgccggcg gagcttgtcc tgcgcaaccc
     6121 actgtgccct ggcctgcgca cgctggttca accacgatgc gaagacagat gtgagcccgc
     6181 cgatggcggc tcccgccagc gccgcgaggg cggtaattac cgatgcatcc ataaggctct
     6241 cacgatgctg gcgaagcgtt ttctgaaacc atgggattga ccggctcggt gttccggacc
     6301 atctgccaca gcccgatcag gttatagaaa acggccagcg cgcaaagcgt gaaaagttcg
     6361 gacttggccg cggcaagcgg ggccccaagc tgcgacagtt tcacgaaacc gtcgatggcg
     6421 gaggtgctcg gcaccaggaa cgatatgagc ctgatgaacg gagggatcgc ctcgcttggc
     6481 caggagaagc ccgcgatcat gaacagcgga agcccgaggg ctgccaaaat aagggccacc
     6541 ctgatcggcc ttcgaaggat gcccgccacc acaaagccaa gtccagagac cgatagcacg
     6601 aagggaacgg cgaaaatcaa aatcggcacg atgacgccaa gccgcggcag cccgtacaga
     6661 tagggcagta cgatgagata tatcggcagg atcacggctt cgagcgcgag gtaggccagt
     6721 agttttccgg ccatgagcgt gaccggcgag aggcgccggc ggcgcgcgtc gtctccggcg
     6781 aaatcggcac ccggcaaggt gccgagcagc ccgacgccca tcaagagcat ctgctgcaga
     6841 atgagtacga acgcggccgg aagcacatag gtggcatagc cggcctcggg attgaatatc
     6901 ggtaccgcgg tcagaggcat gggatccggc gcgacgacgg cgatcgcggg atcgacgccg
     6961 atggcgatca ggcgagcggc ttcgacttcg gcaccgaccg tgcgcgccac agcgccgaca
     7021 gctcccgcta cgcgctggta gatcagaaag taactggcgt cggcatagag cgcgaccggc
     7081 gaaggccgac cgtgcataag gtcgcgctcg aaatattgtg ggatcaccag gatgccatcg
     7141 accctgcgcg caaagacctc gcgctccgcg ctcgcgaggt ccggcaatac ctccgtgatc
     7201 gcgacgtcgg gagtggcgtc gaccagacgg gcgaagtccc ggctgctctg cgttccatcg
     7261 cgatcgacca cggcgatcgg gacgttacgc aaggcctcat tcaaataggg ttggggatag
     7321 aacagggcat agaacgcggc gcccaggatg agcacggaaa acgccggctt gaccgtaaag
     7381 atgcgacgaa gctcattccg aaaagcgtgg agaaggccgc tcacgacggc accttggcaa
     7441 gcggctgcgg agcgttgcgg tcctcgatcc gatgagtgga cctgcgcatg ctctccagct
     7501 tccacgcgac aagtccggcg agcccgaccg cgaacgcggc gagcacgaac agcggtttga
     7561 acgaaaggtc gaccggcgtg ccgcgtatcg tctggtcgat tcgcgcggtc aggtaccacg
     7621 ttcccggcag cagctcgccc caggcatagg cgaaagcatt catgccgagg cgcggaaaac
     7681 caattcccat gaagccgaac gcgggcgccg ttaccagggt ggcgatgctc accgcgctga
     7741 gcgtcggctt tagcagcacc gccagcagcg cgcccagaag ctgacatgac aggatgaaca
     7801 gcacgcccgt catgatcagc agccagggat gcccgcgaag gggcagcccc aagacgacaa
     7861 acagcagcgt gtccgacaga cccagcacca gcaggaaaag tatggtgtaa ggcaatatct
     7921 tgcccgccat ggctggccaa agtccgcccc ccagcgaccg aagaattcga agccggtagg
     7981 gcgtctcgac gtcgaggcca acggaatagg ccgatgtcgt caccatgacg acctgcaata
     8041 cgctcggtat cagggcggtc agcaggaaga atacatagtt cagcgtcggg ttgaacagcg
     8101 catgggtttg aaccggaatt ggctgtagat ccgcctccgc cagatcgagc ggctcgccct
     8161 gagcactgcg cagtgtcagc cggattccgg cagctgcggt cggaactgcc gcgttcacgc
     8221 cgcgagccgt aaggttgccg gtggtcaggg tttgcgtgtt gtagaagaac acgacctccg
     8281 gccggcggcc cgcgaacaca tcgcgctgga gataaagcgg cagcatcaac agtccgtgga
     8341 tttttcctga caggatcagg ctgcgccctt ccgcaagatc gccgacgcgt accgccacgg
     8401 cggagtccgg ggttgcgtcg accatccgga tgatggtgcg cgacaggtcc gaactgtcga
     8461 gatcgagcac gccgatcggc agtcgcgttg ccagtccggc gctgaagacc gcggcgagga
     8521 aggccatctg ggtcagcggg acaatggtcg caagcccaag caggaaaggc cgccgccaca
     8581 gccagcccac ctctcgccag aacaccagcc ggaaaccggg tcggatgccg ctgcgggcca
     8641 gcatgtcaac gccctcgatt ggcccatgaa acgatcgcgc tcatgcccgg acgcaggccg
     8701 tcgaccggct gtatcggcag cgcgcgtact tcgaaggtgc ggagatcgaa atcaccggtg
     8761 gcgcgggtgg cccgccacgc ggcgtattgc ccctgcacat tgatcatggt gacccggacc
     8821 ggaatgactt gggacttcaa agccggcacc gtcaggtcga atttgtcgcc gatcttcaac
     8881 ccgccgagca aatcctcgcg caggttgaat gtgaaccaca ggttctgcag gtcgatcatc
     8941 gagaacagcg gcgccccggc gctgaaattc tcgccgagcg cagcgacccg tgccgtaatc
     9001 tgaccggtga tcggagagcg aatggtgaat tcggccacgt cgacatcgcg ctgattcaaa
     9061 gtggcttctg cctgcttcac ctgtgccgcg gcgagagcac gctcttcctt gctggcgcct
     9121 gtcgtcgcaa gctgtagtgc agcttccgcg gattcgcgtt tgcgggtcgc ggtgtcgaga
     9181 ttgcgggtta cctcatcgac gcgcgcctgg ggtgcgttgc cggtgcgcgc cagctcgatt
     9241 tgccgattgt aggtttcctg ggcaagcgtc tcatcggcct ttgccgcatt gaggtcggcc
     9301 tggcgcgccg cgatcgtctc gggccgggtg ctgttgatgc gatccagatc ggcctttgcg
     9361 accgccaacg cggcttgagc ggcgagccgg gctgcgacca gttgcggact ttccagctca
     9421 gccatgaccg cgccacgctc catggtgtcg ccgacatcgg ccttcagcgt gacgatccgc
     9481 gctgcaaccc gcggactgat gtcgacccga tcagccgaaa cctcgccctg aatgatcagg
     9541 ggcggcggcc gggtcgcaag ccaaagcatc gttgcaaagg ccgcgacgac cactaccccg
     9601 atcagccagc gaaccgacct tttacccatc gcgaggcccc cataaatccc cgttgaaaaa
     9661 tcacggcgcg atcagcgtca cacaggcgcg ccgtcgacgc aacgcgcgtg tcgagcagag
     9721 attttaaatc aaatgttaga atccctcggt gtcaatcgct gaaaaggtag acattaagtt
     9781 ggcgccggcg gtaacacttc agcaccgcca aacgcaatca cgcgcccgcg ctggagcatt
     9841 acgatgctcg tcgccaattg ccgcatttcg ccggcgtcgt ggctgacata aaccatgggc
     9901 acgccggcct catcgcgcag ccgcaccaga tagggcagga tctcaatctt gcgctcctca
     9961 tccagcgatc ccagcggctc atccaacagc agcaatcgcg gtttcgccaa cagcgcgcgc
    10021 ccgagcgcca cgcgctggcg ctcgccgcct gagagttggc cgggacggcg atcaagcaga
    10081 ccgccgatat cgagcagagc ggtgacgcgc gcgcgctcgg cgtgatcatc ggcaaggcgg
    10141 ttcatccgcc gcccatagtc gaggttctgg cgcacatcga gatgcggaaa cagccgcgca
    10201 tcctggaaca cgtagccgat acggcgacgg tgcgccggca cgtgcacgcc cgcagcggta
    10261 tcgtcgagcg tctcgtcatc gataacgatg ctgccgcgat ccggccgcag caatcccgcg
    10321 atgatgttga ccagcgaggt cttgccggca cccgaggcgc cgaacagccc ggtgacccgg
    10381 ccctcgctga ctagggaggc ttgaagcgag aattcgccga gctgttttgc gacctcgatg
    10441 cgcagcatgg tcaattcccg tgcagccgca gcaaggcgcg gcgcgcgaac cattcggagg
    10501 cgatcatggc gctcatcgcg atcacaatcg aaaccgccac cagccgcagc gccgcggcat
    10561 cgccatccgg cgtctggatc agcgaataga tcgccgacga gatggtctgg gtttcgcccg
    10621 gtatgttcga cacgaaggta atggtcgcgc cgaattcgcc gatcgctttc gcaaagccga
    10681 gcaccatgcc ggccagcacg cccggcagcg ccagcggcag ggtgacggtc aggaacaccc
    10741 gccacggcgc cgcgcccaat gttgccgccg cttgctccag ccgcaggtcc accgcttcga
    10801 tcgatagccg gatcggccgc accagaagcg gaaacgacat gatgccgcag gccaacgccg
    10861 cgccggtcca gcgaaacgcg aacacgatgc cgagatggtc cgcgagccac gcgccgacaa
    10921 ggccgcgcct gccgaaggtc agcagcagca gataaccggt gacgaccggc ggca
//
LOCUS       DATNOI010000076         7060 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_676376,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000076 DATNOI010000000
VERSION     DATNOI010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676376"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VN968_02595"
     CDS             <1..916
                     /locus_tag="VN968_02595"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HXL28171.1"
                     /translation="DLPLRLAEFGVVHRYEPSGAMHGLMRVRGFTQDDAHVFCTEAQL
                     ADECLKINDLILSTYADFGFEGDLTVKLSTRPDKRVGTDEMWDHAERVMATVLAQIEA
                     QGHNHRIKVSINPGEGAFYGPKFEYVLRDGIGRDWQCGTTQVDYNLPERFGAFYIDAD
                     GSKKTPVMVHRAICGSMERFIGILIEHYAGNFPLWLAPIQAVVTTITSEGDEYAKVVA
                     AAARRAGLRIELDLRNEKINYKVREHSLAKIPALLVVGKKEAETHSVSIRRLGSEGQT
                     VMPTDEALVALVEEATPPDIKRARLEAR"
     gene            913..1119
                     /locus_tag="VN968_02600"
     CDS             913..1119
                     /locus_tag="VN968_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28172.1"
                     /translation="MTDEQELPAYPIRAYQLIPDPNRPTVVALAIETDQGHSLFLATR
                     EILEDLGKDLLDRAAKMPLKKDQA"
     gene            1262..1825
                     /locus_tag="VN968_02605"
     CDS             1262..1825
                     /locus_tag="VN968_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HXL28173.1"
                     /translation="MPDAIDFLKIRRSIKPREMGEPGPSPAELETILTIGARVPDHGK
                     LTPWRFIVFEGDARFRAGDVIAKVFARKNPQAPPAEIDIEKRRLTDAPLVIAVVSLTK
                     PHPKVPPWEQELSAGASAMNIVTAATALGYGACWLTGWFAFDRDVLDGLGLKADEKLA
                     GFIHIGTPSKPSEDRPRPVLSDIVTRF"
     gene            complement(1854..2849)
                     /locus_tag="VN968_02610"
     CDS             complement(1854..2849)
                     /locus_tag="VN968_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28174.1"
                     /translation="MAMLIPALAGPAAAQMFSDQPPPVPPGSVPDPGGAISLAPPSGP
                     GSVPTTPPPLTQPSIMQPSIAAAPPAAAPQGATPPSQAVLSLTARYGKDLPAINAGLV
                     WRVFTDRPDENGTFKLIREERGATPNIVLPPGGYVVHVTFGLVSAVRPVTLKSETDRE
                     AFLLPAGGLRIEGRVGTSKIPQNQISFAIYKGSQFEVGERAPLVPSVAAGDVALLPEG
                     TYYIISNYGDANSVVRSDIRVQAGKLTDVIISHRAAVITLKLVGDKGGEALANTAWSV
                     ITPGGDVIKESIGAFPRVVLSEGEYRAIAKNEGKVFERPFNVVNGVDGEVEVIAR"
     gene            3139..4200
                     /locus_tag="VN968_02615"
     CDS             3139..4200
                     /locus_tag="VN968_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXL28175.1"
                     /translation="MLDILIGRGQNGQESRDKVGLGSIRRPVIGLALGGGAARGFAHI
                     GIIRTLIAHGIVPNVVVGTSIGAVVGGAYAAGHLDTLEDWARSLQPRNILGYLDIRLN
                     GSGLIGGDKLAAQLEAAIGQTLIEELPLKFATVATEVRTGHEIWLTHGRIVDAMRASY
                     ALPGIFSPVLVGDRWLVDGALVNPVPVSAARAFGAEIVIAANLSSDVFTHSTTIYAHG
                     PSAEVSVSLAPDAEIEPPRPKRGFGKFFSPERTVKREFFGSGERPGISTVMVDAFNIM
                     QDRITRARLAGDPPDLLISPRVGQIGWFDFHRASDLIAHGARAAERAIDSIQEAIHIL
                     APPAAHRPAASVEKVEKKV"
     gene            complement(4215..4643)
                     /locus_tag="VN968_02620"
     CDS             complement(4215..4643)
                     /locus_tag="VN968_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXL28176.1"
                     /translation="MTVRAILDSKGHQIQSVEPGTKLSAAIKILSERKIGAVLAMSEG
                     RIEGILSERDIVRVLGERGAAVLDEPISAVMTRKVVSCRQADTVAAIMEMMTLGKFRH
                     LPVVEDGKVVGLISIGDIVKWRVREFEMEQEALREYIKTA"
     gene            complement(4849..5637)
                     /locus_tag="VN968_02625"
     CDS             complement(4849..5637)
                     /locus_tag="VN968_02625"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXL28177.1"
                     /translation="MESPPELPPAGPREPILTLPGALTAYVLLLAVIHVVRMLLPFDI
                     DDIVIQMFGFIPKRYDSTLLAITFPGGTGAKVWTFVTYSLLHANLAHIGFNVLWLLPF
                     GSALARRFGAVRFFLFMAVTAVAGALAHLVTHEHAVAPMIGASASVSGTMAAAIRFAF
                     VQGSFLSFSRGDADAAAKVPALSLLRALRNTRVLGFLAVWFGVNIIFGIGSIAIGADG
                     ASVAWQAHIGGFFAGLMLFSLFDPIPRTAPNAADASSTDVSERG"
     gene            5928..6236
                     /locus_tag="VN968_02630"
     CDS             5928..6236
                     /locus_tag="VN968_02630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HXL28178.1"
                     /translation="MNSNSSNQGFVITAFWETKPGEEDSVAGLLRQFLPEARKEAGVK
                     EFQIHQSVTKPREFFFYEVFSAETAFAAHQQTEHFKKYILGEAIPKLAKRERSQFRFI
                     "
     gene            complement(6323..6763)
                     /locus_tag="VN968_02635"
     CDS             complement(6323..6763)
                     /locus_tag="VN968_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28179.1"
                     /translation="MMDLLRSAASVRFDDLVRPLLVANDLTDAIVTAVDGLVHHDKLF
                     AATKRYASPNIGARIKARSLEERTWWSTGSVEFMFLLWLADFPACGCVKLRKALALSA
                     RQQDQAMSAHATRHDKLAANYSAFIKPASIRIWLRANECSPIGS"
     gene            6762..>7060
                     /locus_tag="VN968_02640"
     CDS             6762..>7060
                     /locus_tag="VN968_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL28180.1"
                     /translation="MPAFRDRLHVLSATIIVLAAASTSAIAQKKYDTGATDTEIKIGN
                     IMPYSGPASAYGVIGKAEAAYFKKINAEGGINGRKIKFISYDDAYSPPKTVEQAR"
BASE COUNT         1409 a         2207 c         2196 g         1248 t
ORIGIN      
        1 cgacctgccg ttgcggcttg ccgaattcgg cgtggtgcat cgctacgagc cgtcgggcgc
       61 gatgcacggc ctgatgcgcg tgcgcggctt cacccaggat gacgcgcatg tgttctgcac
      121 cgaggcccag ctcgccgacg agtgcctgaa gatcaacgat ctcattctgt cgacctatgc
      181 ggatttcggt ttcgagggcg atctcacggt aaaactgtcg acccggccgg acaagcgcgt
      241 cggcaccgac gagatgtggg accatgccga gcgcgtgatg gcgacggtgc tcgcgcagat
      301 cgaggcgcag ggccacaacc accgcatcaa ggtttcgatc aatcccggcg aaggcgcgtt
      361 ctacggcccg aagttcgaat atgtgctgcg cgacggcatc ggccgcgact ggcagtgcgg
      421 caccacgcag gtggattaca acctgccgga gcgattcggc gcattctata tcgatgccga
      481 cggctcgaag aaaacgccgg tcatggtgca ccgcgcgatc tgcggctcga tggagcgttt
      541 tatcggcatc ctgatcgaac attatgcggg taactttccg ctttggctgg cgccgatcca
      601 ggccgtcgtc accaccatca cctcggaagg cgacgaatac gccaaggtgg tcgcggctgc
      661 ggcgcggcgc gccggtttgc gcatcgagct cgacctgcgc aacgaaaaga tcaactacaa
      721 ggtccgcgaa cattcgctgg cgaaaattcc ggcgctgctg gtggtcggca agaaggaagc
      781 cgagacccat tcggtctcga tccggcggct gggcagcgag gggcaaaccg tgatgccgac
      841 cgatgaggcg ctggttgcgc tggtcgagga agcgaccccg ccggacatca aacgggcgcg
      901 gctggaggcg cgatgaccga cgaacaggaa ctgccggcct atccgatccg ggcctatcag
      961 ctgatcccgg atcccaatcg gccgaccgtg gtggcgctgg cgatcgagac cgatcagggc
     1021 cacagcctgt tcctggccac gcgcgaaatt ctggaagatc tcggcaagga tctgttggat
     1081 cgggcggcga agatgccgct caagaaggat caagcctagc ggttgcatcg gccgtatcgt
     1141 tggcgtgccg gcgtcgccgg ggcacccgat cacaatcggt ctaaaatgcc cgtccaccgc
     1201 tcgtggccgg cggcaccgcg tgctatgcga tgccccatca ttcccgatga aagaacccgc
     1261 attgcccgac gccatcgact tcctcaaaat ccgccgctcg atcaaaccgc gcgagatggg
     1321 cgagcccggc ccctcgcctg ccgagcttga aaccattctg acgatcgggg cgcgggtgcc
     1381 cgatcacggc aagctgacgc cgtggcgatt tattgtgttc gagggcgacg cgcgcttccg
     1441 cgccggcgac gtgatcgcaa aggtgttcgc tcggaaaaac ccgcaagccc cgcccgccga
     1501 gatcgatatc gaaaaacgcc ggctcaccga cgcgccgctg gtgatcgccg tggtcagcct
     1561 gacaaaaccg catcccaagg tgccgccctg ggagcaggaa ttgtcggcag gcgccagcgc
     1621 gatgaacatc gtcaccgccg ccaccgcgct cggctacggc gcctgctggc tgaccggctg
     1681 gttcgcgttc gaccgcgacg tgctcgacgg gctgggattg aaggccgacg aaaagctcgc
     1741 gggcttcatc catatcggca cgccctcgaa gccgagcgaa gaccgcccgc gcccggtatt
     1801 gtcggatatc gtgacgcggt tttagatcca acttgtcatt tgtgaaaaaa tatctaacgc
     1861 gcgatgacct cgacctcgcc atcgacgccg ttgacgacgt tgaagggccg ttcgaacacc
     1921 ttgccctcgt tcttggcgat ggcgcgatat tcgccttcgg acagcacgac gcgggggaat
     1981 gcgccaatcg attccttgat gacgtcgccg cccggcgtaa tcaccgacca cgccgtgttg
     2041 gccaaggcct cgccgccctt gtcgccgacg agctttagcg tgatgaccgc ggcgcgatgg
     2101 ctgatgatca cgtcggtcag cttgccggcc tgcacgcgga tatccgatcg caccacggag
     2161 ttggcgtcgc cgtagttgga aatgatgtag taggtcccct ccggcaacag cgcgacgtcg
     2221 ccggcggcga cgctcggcac caggggcgcg cgctcgccga cttcaaactg gctgcccttg
     2281 tagatcgcaa acgaaatctg gttctgcggg atctttgacg tgccgacgcg gccttcgatg
     2341 cgcaggccgc ccgccggaag caggaaggcc tcgcggtcgg tttcggactt cagggtcacc
     2401 ggccgcaccg cgctgaccag tccaaaggtg acatggacga catagccgcc cggcggcagc
     2461 acgatattgg gcgtcgcacc acgttcctcg cggatcaatt tgaaggtgcc gttctcatcg
     2521 ggccgatcgg taaaaacccg ccacaccagt ccggcgttga tcgccggcaa gtccttgccg
     2581 taacgcgccg tcagcgacag cacggcctgg ctggggggcg tcgcaccctg aggcgctgca
     2641 gccggtggcg ccgcggcgat cgaaggttgc atgatggaag gctgcgtcaa cggcggcggc
     2701 gtggtgggta cagatcccgg accggacggc ggggccaggc tgatcgcgcc gccggggtcg
     2761 ggaaccgatc cgggaggcac tggcggcggt tgatcggaga acatctgggc cgcagccggg
     2821 cccgccagcg cggggatcag cattgccatg agcacaaccg ctcggagcaa gcgtccgcac
     2881 gcctcgaatc gttcgatgcc gttaccccct gatgtcatgg agcatggttt tcaccgaaaa
     2941 cgcggcaaat tcaagcctgc aggcgtggca gccggcggct cctcggaaac cggcaaaaac
     3001 cccgttgtga tcctgatgtc gcgatccagt cacaatctct gcctacgccc attggcaccg
     3061 cggcataaac catgcttgtg gcacaatctg gcgggaccgg gcgttagtgc atagtcttgt
     3121 gctctcagga gttgtcaggt gctggatata ttgatcgggc gcggccaaaa cggccaggaa
     3181 agtcgcgaca aggtgggtct cgggagtatc cggcgaccgg tgatcggcct cgctctgggc
     3241 ggcggggccg cccgcggatt tgcccatatc ggcatcatca ggacattgat cgcccacggc
     3301 atcgtcccca atgtggtggt cggcacctcg atcggcgcgg tcgttggcgg cgcctatgcc
     3361 gcggggcatc tcgatacgct ggaagactgg gcgcgcagcc tgcagccacg aaatatcctg
     3421 ggctatctcg atatccggct gaacggttcg ggcctgatcg gcggtgacaa gctggcggcc
     3481 cagttggaag ccgcgatcgg ccagaccctg atcgaggaat tgccgctgaa atttgcaacc
     3541 gtcgcgaccg aagtccgcac cggccatgaa atctggctga cccatggccg gatcgtcgat
     3601 gcgatgcgtg cctcctatgc gctgccgggg atattttcgc cggtgctggt gggcgaccgc
     3661 tggctggtcg acggcgcgct ggtgaatccg gtcccggtgt cggcggcgcg agcgtttggc
     3721 gcggaaatcg tcatcgccgc caacctctca agcgacgtgt tcacccacag tacgacgatc
     3781 tacgcgcacg gcccgtcggc ggaggtatcg gtcagcctcg cgccggacgc cgagatcgag
     3841 ccaccccggc cgaagcgcgg cttcggcaaa ttcttctcgc ccgagcggac cgtgaagcgc
     3901 gagtttttcg gcagcggcga acgaccgggc atctctaccg tcatggtcga tgccttcaac
     3961 atcatgcagg accgcatcac tcgcgcccgc ctggccggcg atccacccga cctgttgatc
     4021 tcgccccggg tcggccaaat cggctggttc gattttcacc gcgccagcga tctgatcgcg
     4081 catggcgcgc gcgccgccga gcgcgccatc gactccatcc aggaggcgat ccacattctc
     4141 gcaccgcctg cggcccaccg acccgccgct tcggtcgaga aggttgaaaa gaaggtctga
     4201 gcgaacgcgt ccgctcaggc ggtcttgatg tattcgcgca gcgcttcctg ctccatctcg
     4261 aattccctga cgcgccattt gacgatgtcg ccaatcgaaa tcaggccgac caccttgccg
     4321 tcctcgacga caggcagatg cctgaattta cctagggtca tcatctccat gatggcggcg
     4381 acggtgtcgg cctgccggca actgacgacc ttgcgcgtca tcaccgcgct gatcggttcg
     4441 tccagcaccg cagcaccccg ctcacccagc acgcgcacaa tatcgcgctc cgacaggatg
     4501 ccttcgatgc gaccttcgct catcgccagc acagcgccga tcttgcgctc cgacagaatc
     4561 ttgatcgcag ccgaaagctt cgtgcccggc tcgacgctct ggatctgatg gcctttggaa
     4621 tcgagaattg cacgtaccgt catggtcgtc tccctaagtg gtcgtctccc tgaatctgat
     4681 cgcgcccttg agcgagcgcg gtctttgttg cttttgagta tgcaatcaac agtttagcgc
     4741 caattttgtt gcaggcgcat tgtaagccgc ggatatctaa gcacgctcgc gggcttatgt
     4801 cgtaactctg tcataatgta tgaaatcgac ttgcgccgca agcgacgatc agccgcgttc
     4861 cgatacatcg gtggacgacg catctgcagc attgggcgcg gtacgcggga tcgggtcgaa
     4921 cagcgaaaac aacatgaggc cggcgaagaa gccaccgata tgcgcctgcc aggcgacgct
     4981 ggcgccgtcg gcgccaatcg cgatcgatcc aatgccgaag atgatgttga cgccgaacca
     5041 gacggccaga aaacccagga cgcgcgtgtt gcgcaacgcg cgcagcagcg acagcgccgg
     5101 cacctttgcc gcggcatcgg cgtcgccgcg gctgaatgac agaaaactgc cctgcacgaa
     5161 ggcgaagcgg atcgcggccg ccatcgtgcc ggacaccgac gccgaagcgc cgatcatcgg
     5221 cgccaccgcg tgttcatggg tgacgagatg cgctagcgcg ccggccacgg cggtcaccgc
     5281 catgaacagg aaaaagcgca ccgcgccgaa gcggcgcgcc agcgcgctgc cgaacggcag
     5341 cagccacagc acgttgaagc cgatatgggc gagattggcg tgcagcagcg aataggtcac
     5401 gaaggtccag accttggcgc cggtgccgcc cggaaacgtg atcgccagca gcgtcgaatc
     5461 gtagcgtttc ggaatgaagc cgaacatctg gatgacgatg tcgtcgatat cgaacggcaa
     5521 cagcattcgg acgacgtgaa tcaccgcaag cagcaggaca tacgcggtca aggcgccagg
     5581 cagcgtcagg atcggttcgc gcgggccggc cggcggcaat tctggcgggg attccaaggg
     5641 ctcaaacacc taaaacggcc gcctcacgcg accgattgcg ccccattagg cggcttggcg
     5701 ctgcggcgca agggcgcaaa cccgtccggg gcgctgaggc cgaatgcatc gatcgaccca
     5761 gaaccgtcat ccatcgtcaa agacaccgct ttttggactt tccgtccggg tccgtctggg
     5821 cggcgttggt tcggcgcgca ccggagcgtt cggaccgggg ggcgtgccaa tctcagcccg
     5881 atcgcccctt gaggcgaatg gctcgtcagc atctacggag aaaaaccatg aattcgaata
     5941 gctccaacca gggtttcgtc attaccgcat tttgggagac caagccgggc gaggaggatt
     6001 ccgttgcagg cctgttgcgc caatttcttc ccgaggcgcg gaaggaagcg ggcgtgaagg
     6061 aattccagat tcatcagagc gtaaccaaac cgcgggagtt cttcttctac gaagtatttt
     6121 cggcggaaac cgcgttcgca gcccaccagc agaccgagca tttcaagaag tatatccttg
     6181 gcgaggccat ccctaagctt gccaagcgtg aacggtcgca gtttcgattt atctgagcgc
     6241 cgacccgcgc acagagcaag atccgccctg ggacaaacgc caagcgtttg cccggagatc
     6301 atgctcaaac aaggcgatga ggtcatgatc cgattgggct gcactcgtta gcgcgtagcc
     6361 aaatgcggat tgatgcgggt ttgatgaagg ccgaatagtt ggctgcgagc ttgtcatgtc
     6421 gggtcgcatg cgccgacatt gcttgatctt gttgccgggc tgaaagggcg agcgctttgc
     6481 gcaatttgac gcacccgcag gcaggaaaat ctgcaagcca taacagaaac atgaactcaa
     6541 ccgagcccgt actccaccac gttcgctcct caaggctgcg ggcctttatc cttgccccga
     6601 tgttcggcga ggcatagcgt ttggtggcgg caaagagttt gtcgtggtga accaagccat
     6661 caacagcggt tacgatggca tcggtcaagt cgttggccac aagaagggga cgtacaagat
     6721 catcaaaacg aactgatgca gctgaccgaa ggaggtccat catgccagct tttcgtgatc
     6781 gactacacgt gctgtcggca accattattg tgttggcagc agccagcacc agcgcaattg
     6841 cccagaagaa atacgacacc ggcgcaacag ataccgaaat caaaatcggc aacatcatgc
     6901 cttacagcgg accagcttcc gcttacggtg tcatcggcaa ggcagaggcc gcgtacttca
     6961 agaagatcaa cgcagagggc ggcatcaatg gccgcaagat taaattcatc tcatatgacg
     7021 acgcctacag cccgccgaaa accgttgaac aagcccgcaa
//
LOCUS       DATNOI010000077        11297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1735421,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000077 DATNOI010000000
VERSION     DATNOI010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735421"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VN968_02645"
     CDS             <1..549
                     /locus_tag="VN968_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL28181.1"
                     /translation="GGAGSWITLVIGVTWRLTSMRSKSFDGMACSIAGVLDALGDRWA
                     VLILRDLSLGLSRYEDLRRSTGVTHATLSDRLKHLEEKQLIERRRYQTGPERHEYLLT
                     RKGRDTILVMQALAQVGDKWAVSGDAGPPLKFVNRKNGRTLKLALVEEKSGELVGAKD
                     VQPQAGPGADDLVRWRLTKFGN"
     gene            1091..2290
                     /locus_tag="VN968_02650"
     CDS             1091..2290
                     /locus_tag="VN968_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL28182.1"
                     /translation="MFRALKIGFALAIAVGSSALAVDNPGVTPTEVKVGGIFPFSGPA
                     SSIGLVGRGILAYVQSINDRGGINGRKINYIAYDDAYSPPKAVEHARKLVESDEVAFM
                     FSQLGTPGNSATAKYLTSKGVPAIAIVSGSNKFTNVADFPLTTTSLVSFDTEGKIYAK
                     FLTRTLPNAKYAILYQNDDLGKDYVNAFKALLKSDFDKRVVVAAYEITDPTVDSQVVN
                     LKSSGAEALLVAGTPKFAAQAIRKASEIGWKATILLNYPSSSVGATLKPAGLDKSTGV
                     IVGTITMDPTDSQWDNNEGMKNFRAFVDRYMPGVDIADTNYLFGYTQGMLLEQIIKQC
                     GSDLSRENIIKQARNLRDVVLPTALPGIRINTSDKINMDFTQMRLQRWTGTRWDQFSD
                     VLDAASE"
     gene            complement(2366..2881)
                     /locus_tag="VN968_02655"
     CDS             complement(2366..2881)
                     /locus_tag="VN968_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL28183.1"
                     /translation="MKWDALEEEPCSLARTIGVIGDRWSLLILRECFLRTRRFEGFQS
                     ALGITRHLLAERLKKLVRQGVLRRVPYQESPKRHEYILTQKGLDLYPVMMALVHWGDT
                     HMVDERGRPLLHEHRNCGKTFDPVMVCSECGEPLLAKQVHTHPGPGARQASAKLPPDK
                     PAKPRTRKQAA"
     gene            3137..3823
                     /locus_tag="VN968_02660"
     CDS             3137..3823
                     /locus_tag="VN968_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HXL28184.1"
                     /translation="MVTPLKVEFQFDFGSPNAYLAELALAGIELRTGVKFEYVPVLLG
                     GIYKATNNMSPAESLRGIRNKPEYQALETQRFIRRHNITKFRQNPLFPVNTLMLMRGA
                     VAAQFEGVFEPYFRAAYHHMWEEPKKMDDLEVFRSAFISSGIDIEKLMARAQQDEVKK
                     KLIELTTDAVNRGAFGSPTRPEIATRGLSASGGYPRLERPEWIIVTAAERNSRRMSCP
                     VHSTAFACSI"
     gene            3784..4983
                     /locus_tag="VN968_02665"
     CDS             3784..4983
                     /locus_tag="VN968_02665"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXL28185.1"
                     /translation="MPGPLNGVRVLDLTGVVSGPFATMFLADQGADVLKIEPIGGDIT
                     RRSRATIDKAGEFSALFISSNRGKRSLSIDVKSAVGREVLAKLIAQADVLVQNFRPGT
                     MERLGLGADELRERHPRLIYVSISGVGDTGPYVKKRVYDPIIQGLSGFADIQSQPVTN
                     RPQMIRTIVCDKTTAVFTAQAVSSALYAREKTGKGDHIQVAMLDAMISYLWPEGMMQY
                     TVIGSEATSADPNDRPDLVFKTSDGYITAGTISDSEWQGFCRASGDPELARDPRFATP
                     SARSVNATARINKMAEYIAERTTAEWLERLDAADVPCAPILRRGEIIHNEQVVARGII
                     AEFDQPAVGRVRQPKPAARFSVNEAVIGGPAPRVGEHSRDVLRDLGYDDGAIDKMVSE
                     KSVRVAV"
     gene            complement(4980..5666)
                     /locus_tag="VN968_02670"
     CDS             complement(4980..5666)
                     /locus_tag="VN968_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXL28186.1"
                     /translation="MGATPDLTLWGVGTSRTIRPHWAMHELGLSYKTKPIGPRTGETK
                     TAEYTKLNPRQKVPLLQDGDFCIGESAAIVAYLSRMYSTPERALIPKTPREYASWLEW
                     CFFVVAELDSTSLYVMRRHRADALGHIYGVAPEVVAQAGEYFRQQLRHVETAFADGRT
                     FLMGDKFTSADILLTTCLDWAIAYGVGICDNAHPYLERIQARPAYQLAKAANVPLAPI
                     VPVQQKILPD"
     gene            complement(5666..6409)
                     /locus_tag="VN968_02675"
     CDS             complement(5666..6409)
                     /locus_tag="VN968_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL28187.1"
                     /translation="MVESRGVAILVGAGDAIGAAVARRFAKGGYTVCICRRDAAKSRG
                     LVDELNAAGHGAHAFSVDARQEAEVQKLFADVEQNIGPIEICLYNAGSNVNKPLLETT
                     EKLFFKAWELACYGGFLVGREAARYMLARGRGTIFFTGATASVRGGKGFAAFSSAKFG
                     LRAVAQAMARELGPKNIHVVHLLIDAGVDSEAIHQRMKAARGIEASEIPPDSLTKTSS
                     IAEAYWFAHQQTRDGWTHELDLRPSVESW"
     gene            6639..8195
                     /locus_tag="VN968_02680"
     CDS             6639..8195
                     /locus_tag="VN968_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXL28188.1"
                     /translation="MTKQSEAIDLAEIVTGLPSRMHHVADGHVAESPDHVALIEDGSS
                     WSYRKLDRSVKEIAAVLASLGVRPGDRVIIVSENCIALAALLLAASRIDAWAIVANPR
                     LSARELDQIRDHSGARRVFFTVAVSKEAAAHASRCAAKIGQFGPLREIGVSTLNESAT
                     AEPVETDPAQQVAVLIYTSGTTGAPKGVMLTHENLLISAKTTAHFRKMDAGDKVYVVL
                     PISHIVGISLLIMTLMVGGTVRLVSKYDPAALAGAIAEEGITILNGVPATYQRLLEYK
                     AVTGLAQLDRGSLRLIAVAGAPLDLDLKSGVEREFGLPLSNGYGITECSPGISGVRFD
                     APRADQAVGTLLPGIEARIARLDGGMVPNGEIGELHVRGRNVMRGYYRAPELTAKAID
                     TDGWFNTGDLARFDGDCLYIVGRSKEMIIRSGFNVYPAEVEAVLNSHAAVVQSAVVGR
                     AVEGNEEVVAFVQLMQGSPVKPAELMAFINPQLTSYKRPSEIIVLDALPATSTGKILK
                     HKLAESLRGA"
     gene            8262..8999
                     /locus_tag="VN968_02685"
     CDS             8262..8999
                     /locus_tag="VN968_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL28189.1"
                     /translation="MQKRNATVAVIGAGDYIGGEIAKKFALEGFTVFAGRRNGAKLEP
                     LVKEIEAAGGEIHARSLDARKEEEIISFLGDADRHAPLEVCIFNVGANVNFPILDTTE
                     RVFRKVWEMACYSGFLAGREAARLMLPRGQGNIFFTGATASLRGGAGYAAFASAKFGL
                     RAVAQATARELGPKNIHVAHLIIDSGVDTEWVRQRRLEALGPGALDNPDLLMPPSSVA
                     ESYWQLYQQPRSAWTFELEIRPFGEKW"
     gene            9066..10067
                     /locus_tag="VN968_02690"
     CDS             9066..10067
                     /locus_tag="VN968_02690"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HXL28190.1"
                     /translation="MKTAITEMFGIEHPIIQGGMHYVGFAELAAAVSNAGGLGIITGL
                     TQRTPDLLAREIARCHDMTDKPFGVNLTFLPAFSAPPYPEYIAAIREGGIKIVETAGR
                     SPEQYMPALKAAGIKVIHKCTSVRHSLKAEQIGCDAVSVDGFECGGHPGEDDMPNMIL
                     LPRAAEELKIPFVASGGMADARSLVAALALGAAGMNMGTRFIATREAPVHENVKQALV
                     AATELDTRLVMRALRNTERVLRNAGVDRLIEIEREKGRSLRIEDIHDEVAGVYPKVMI
                     DGDLDAGAWSCGMVVGLIKDIPTVKELIDRIMADAEHIIRSRLTGFLDDGKATRRAA"
     gene            10153..10926
                     /locus_tag="VN968_02695"
     CDS             10153..10926
                     /locus_tag="VN968_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXL28191.1"
                     /translation="MTDHVRREIADGIMTLTISRSDKKNALSNAMYSALSDGLELAEQ
                     DPSVRVVLFQGDGDSFTAGNDLADFSAEASGKSVGESQAARFIGNLARATVPLVAAVQ
                     GNAVGVGTTMLLHCDLVYLADNAKLITPFVNLALVPEASSSWLLPARIGHARAYAMFA
                     LGEPMDASSALACGLANAVVPLSELRTKAHGAAAALTKRPAGSLKLTKALMRETDRIV
                     AQIAREGALFRDRLQSGEAREAFAAFAEKRKPDFSKVSG"
BASE COUNT         2257 a         3376 c         3519 g         2145 t
ORIGIN      
        1 ggcggcgccg gaagttggat tactctggta ataggagtta cctggaggct gacgtcgatg
       61 cgatccaaga gttttgatgg aatggcgtgc tcgatcgccg gcgtgctgga tgcgctgggc
      121 gaccgctggg ccgtgctgat cctgcgcgat ctgtcgctcg ggctcagcag gtacgaggat
      181 ctgcgccggt cgaccggcgt cactcatgcc accctgtccg atcggctcaa gcacctcgaa
      241 gaaaagcaac tgatcgagcg gcggcgatat caaacaggtc ccgagcgtca tgaatatctg
      301 ctgacccgaa agggcaggga cactatcctg gtcatgcagg cgctggcgca ggtcggggac
      361 aagtgggcgg tgtccggcga cgcggggcca ccgctcaagt tcgtcaatcg aaagaacggc
      421 cggacgctga agcttgcact ggtcgaggag aagtcgggtg aactggttgg cgcgaaggac
      481 gtccagccgc aggccggtcc cggtgcggac gatctggtcc ggtggcgcct gacaaaattc
      541 gggaactgat gttgtttggg cgttgctgcg tcgcgttatt gcgagcagcg gtgcgaccga
      601 gcaatccggt agctgcctca tccattctct ggatcgcttt gcagagcttg gcgtttgcgc
      661 gtattcgcga gcgccaccga tagtcttgaa aaagaactat gaaatcagcg atagactgaa
      721 acctcgcgtc ggcagcgaag gccggtcctt tcagttatga gggatctggc gcgagcagat
      781 acccatgccc aaatttccgg taaaggctca tataattggt gtaaaattac atcgacaatc
      841 actgcgatcg cgccttggat tgtgctgcgg ttgacgtccc aaacggtggc gatcgtgggc
      901 ttgctgtgac aatcgcccac ccgaagcctt gactagttca aaaaaagaac cgtagattgc
      961 gaggggccga tggatggcgg cgagttcaat tccggttctc gcgttgtgag ctttcgttgc
     1021 tgccgccgcc cataaaaacg atgcaccgcg ccccctggca gcggacgccg gcaaatcgga
     1081 ggagagcgca atgttcagag cattgaagat cgggttcgcg ttggcgatcg cggtgggatc
     1141 gtccgcattg gcggtcgata accccggtgt cacgccgacc gaggtcaagg tcggcggcat
     1201 ctttcctttc agcgggccgg cctcgtccat cggactcgtc ggccggggaa tcctcgccta
     1261 cgttcaatcc atcaacgacc gcggcgggat taacgggcgc aaaataaact acatcgctta
     1321 tgacgacgcc tatagcccgc cgaaggcggt agaacatgcc cgcaagctgg tcgaaagcga
     1381 cgaagtggct ttcatgttca gccagttagg tacgccggga aactcggcga cggcaaaata
     1441 cctgacctcg aaaggcgtac cggctatcgc catcgtctcc ggatcgaaca agttcaccaa
     1501 cgttgcggat tttccgctga ccacgaccag tctggtcagc ttcgataccg aaggaaaaat
     1561 ctacgccaag tttctgaccc ggacgctccc gaacgcgaaa tacgcgatcc tttatcagaa
     1621 cgacgatctc ggcaaggact acgtcaatgc cttcaaggcg cttctgaaaa gcgactttga
     1681 caagagggtt gtggttgccg cctatgaaat caccgatccg accgtggatt cgcaggttgt
     1741 taatctgaaa agctccggcg ccgaggcgct gctggtggcg gggacgccga aattcgccgc
     1801 gcaagcgata cggaaagcct ccgaaatcgg ctggaaggcg accatcctcc tgaactaccc
     1861 gtcaagttcg gttggtgcga ccctgaagcc cgccggcctt gataaatcca ccggcgtcat
     1921 tgtcggaacc atcacgatgg atccgaccga ctcgcagtgg gacaataacg aggggatgaa
     1981 gaatttccgg gcctttgtcg acaggtacat gcccggcgtc gacatcgccg atacaaatta
     2041 cctgttcggt tacacccagg gcatgctgct cgagcagatc ataaagcagt gcggcagcga
     2101 tctgtcgcgc gaaaatatca tcaagcaggc caggaatctc agggatgttg tcctgccgac
     2161 ggcgctgccc ggcatcagga tcaacacgtc cgacaagatc aatatggact ttacccagat
     2221 gcggctgcag cgctggaccg gtacgcgctg ggatcagttc agcgacgttc tggacgccgc
     2281 gtccgaataa tcgcgcgggg ttccggcgct aatgttgcga cagggcgaaa caccaatcca
     2341 gtgctgtttt tcaaatggct gctgttcaag ccgcttgctt gcgcgtcctc ggcttcgcag
     2401 gcttgtcagg aggcagcttt gccgaggctt gccgcgcgcc gggacccgga tgggtatgaa
     2461 cctgcttggc caaaagcggt tcgccgcatt cggagcatac catcacggga tcgaacgtct
     2521 tgccgcagtt gcggtgctcg tgcagcagcg gccggccgcg ttcgtcgacc atgtgggtgt
     2581 cgccccaatg gaccaatgcc atcatgaccg gatacagatc gaggcctttt tgggtgagaa
     2641 tgtattcgtg gcgtttgggc gactcctggt agggaacgcg gcgcaagaca ccttgccgga
     2701 ccagcttctt cagccgttcc gcgagcagat ggcgcgtgat tccgagcgcg gactgaaatc
     2761 cctcgaaccg ccgggttcgc aggaagcatt cccgcaagat cagaaggctc cagcgatcgc
     2821 cgatcacgcc tatggttcgg gccaatgaac atggctcttc ctccagggca tcccacttca
     2881 ttcctgctct ccaatcacgg cgcgtcgata tccgctcgaa tcggctggca atccacggcc
     2941 gtcccgccat tctaaatagg cactcagctc gagacaatta cctgagcggg cgatgtactt
     3001 gcaataaact accctaaaca ggcgttcatt gacagttcgt ttttagaact gtaagaggaa
     3061 tctatagcca aggcacggct gctggtcgtg gcgcgaccag ccgttacaga atgacgcgtg
     3121 ccgtgaggag aggccggtgg tcacccccct gaaagttgaa tttcagttcg atttcggcag
     3181 cccgaatgcc tatctggcgg agcttgccct tgccggtatc gaattgcgca cgggcgtgaa
     3241 gttcgaatat gttccggtcc tgctcggcgg gatctacaag gcgaccaaca atatgtcacc
     3301 ggccgagtcg cttcgcggaa tcaggaacaa gcccgaatat caggcgctgg agacccagcg
     3361 tttcattcgc cgtcacaaca tcacgaaatt ccgccagaat ccgttgtttc ccgtcaatac
     3421 gctcatgctc atgagaggtg cggtcgcggc ccagttcgaa ggcgtgttcg agccgtattt
     3481 ccgcgccgcc taccatcaca tgtgggagga gccgaagaag atggatgacc tcgaggtttt
     3541 tcgaagcgca ttcatctctt ctgggatcga tatcgaaaag ctgatggcgc gcgcgcagca
     3601 ggatgaggtc aagaagaagc tgattgaatt gacgaccgac gcggtcaacc gcggagcatt
     3661 tggttcgcca acacggcctg agatcgcgac ccgcggccta tcggcatcag gtggatatcc
     3721 gcggcttgaa agaccagaat ggattatcgt gaccgctgcg gaacgtaact caaggagaat
     3781 gtcatgcccg gtccactcaa cggcgttcgc gtgctcgatt tgacgggtgt ggtctcgggc
     3841 ccgttcgcga ccatgtttct ggcagaccaa ggcgccgacg tgctcaagat cgagccgatc
     3901 ggcggcgaca tcacccgccg cagccgcgcc accatcgaca aggctgggga gttctccgcg
     3961 ctctttatct cgtcaaaccg tggcaaacgc tcgctgtcga tcgacgtcaa gagtgcggtc
     4021 ggccgcgagg tcctcgccaa gctcatcgcg caagctgacg tgctggtgca gaatttccga
     4081 cccggcacca tggagcgcct tggcctcggc gccgatgaat tgcgcgagcg ccatccgcgc
     4141 ctgatctatg tttcaatcag cggcgtcggc gacaccggac cctatgtgaa aaagcgggtc
     4201 tacgatccga tcattcaggg tctgtccggc ttcgccgata tccagtcgca gccggtgacc
     4261 aatcgtccgc agatgatccg caccatcgtc tgcgacaaga ccaccgccgt ctttaccgct
     4321 caggccgtgt cgtcggcgct gtacgcgcgc gagaaaaccg gcaaaggcga ccatatccag
     4381 gttgcgatgc tcgatgccat gatctcgtat ctgtggccgg agggcatgat gcagtacacc
     4441 gtgatcggat ccgaagcgac cagcgccgat cccaatgatc ggccagatct cgtgttcaag
     4501 accagcgacg gctacatcac ggccggcacc atttccgatt ccgaatggca gggcttctgc
     4561 agggcatccg gcgatccgga acttgccaga gatccgcgct ttgcgacgcc gtcggcgcgt
     4621 tcggtcaacg ccaccgcgcg catcaacaag atggccgaat atatcgccga gcgtaccacc
     4681 gcggaatggt tggagcggct cgacgccgcc gacgttccct gtgcgccgat cctgcgccgc
     4741 ggcgagatca tccataacga gcaggtggtg gcccgcggta tcatcgccga gttcgatcag
     4801 cctgcggtgg ggcgggtgcg gcagccgaag cccgcggcgc ggttctctgt caacgaggct
     4861 gtcatcggcg gaccggcgcc ccgcgtcggc gaacattccc gcgatgtgct gcgcgatctc
     4921 ggttacgacg acggcgccat cgacaagatg gtcagcgaaa agtcggtccg ggtagcggtc
     4981 taatccggca agatcttttg ctgcaccgga acgatcggcg ccagcgggac attcgccgcc
     5041 ttggcgagct gataggccgg tcgcgcctgg atgcgttcga gatagggatg cgcgttgtcg
     5101 caaatgccga cgccgtaggc gatcgcccaa tccaggcagg tcgtgagcag gatgtcggcg
     5161 ctggtgaact tgtcgcccat caggaacgtg cggccgtcgg cgaaggcggt ttccacatgg
     5221 cgcagttgtt gacggaagta ttcaccggcc tgcgcaacaa cttcgggcgc aacaccatag
     5281 atatgtccca atgcgtcggc gcgatggcgg cgcatcacgt agaggctggt ggagtccagt
     5341 tccgcgacaa caaagaagca ccattccagc cacgacgcat attcccgcgg tgttttggga
     5401 atcagcgcgc gttccggtgt cgagtacatt cgcgagaggt aggccacgat cgcggcactt
     5461 tcgccaatgc agaagtcgcc gtcctgcagc agagggacct tttgacgggg attgagcttg
     5521 gtgtattcgg cggtcttggt ctcgccggtc cgcggtccga tcggcttcgt cttgtacgac
     5581 aagcccagtt cgtgcatggc ccagtgggga cgaatggtgc ggctcgttcc aactccccac
     5641 agcgtcaggt ccggtgtggc gcccatcacc agctctccac ggatggacgg agatcgagtt
     5701 catgggtcca gccgtcccgg gtttgctgat gggcgaacca gtaggcctcg gcgatggaag
     5761 acgttttcgt caggctgtcg ggagggattt cgctggcttc gatgcctctc gccgccttca
     5821 tgcgctggtg aatcgcctcg ctgtcgacgc ccgcgtcgat caggagatgc acgacgtgga
     5881 tattcttggg cccgagttcg cgcgccatcg cctgagccac cgcgcgcagt ccgaatttcg
     5941 ccgacgaaaa tgccgcaaag cccttgccgc cgcggacgct ggcggttgcg ccggtgaaga
     6001 agatggtgcc gcgcccgcgc gcaagcatgt accgcgccgc ctcgcggccg accagaaacc
     6061 cgccgtaaca ggcaagctcc caggccttga agaacagctt ctcggtggtc tccagcaggg
     6121 gcttgttgac gttggatccc gcattgtaga ggcagatctc gatcggtccg atgttctgtt
     6181 cgacatcggc gaacagcttc tgaacctcag cttcctgacg ggcatcgacg ctgaaagcgt
     6241 gagcgccgtg gcccgcggca ttcagctcgt cgaccaatcc ccgggatttg gccgcgtcac
     6301 gccggcagat gcagacggta taaccgccct tggcaaaacg ccgggcgacg gcggcgccga
     6361 tggcgtcgcc tgcgccaaca agtatcgcta cgccgcgact ttccaccatc gttgctctcc
     6421 cagtaagttc ttatttagta cctaataaat accggagcct ggagtaattg taaatgtaat
     6481 tcgcaggtaa gtctcgaccg tgatagctcc tgccgcgatt cttcaccaag cggtgaaggc
     6541 ctggcagcga atagagattg acgagttcta aaaaagaacg ttaataacag gacgataggt
     6601 cgccgtgaaa agcggcgcga tggatcggga ggcgggtcgt gacgaaacag tcggaagcga
     6661 tcgatctcgc cgagatcgtc accgggcttc cgagccgcat gcatcacgtc gctgacgggc
     6721 atgttgccga atctcccgac catgttgcgc tgatcgagga cggcagctcc tggagctatc
     6781 gcaaactgga tcgctcggtc aaagagattg ccgcggttct cgcctcgctc ggcgtcagac
     6841 ccggcgaccg ggtgatcatt gtcagcgaaa actgcattgc gcttgccgcg ttgctgctgg
     6901 cggcgagccg gatcgatgca tgggcgatcg tcgccaatcc gagattgtcg gcgcgcgaac
     6961 tggatcagat ccgcgatcac agcggcgcgc gccgggtgtt ctttacggtt gcagtctcga
     7021 aggaagcggc ggctcacgct tcacgttgtg cggctaagat cggccaattc ggcccgctga
     7081 gagagatcgg cgtcagcacg ctaaacgaaa gcgcgacggc cgagccggtt gaaaccgatc
     7141 ccgcgcagca ggtcgcggtg ctgatctaca cctcgggaac gacaggtgcg ccgaagggcg
     7201 tgatgcttac ccatgaaaac ctgctcatca gcgcgaaaac cacggcgcat ttccgaaaga
     7261 tggatgccgg cgacaaggtg tatgtcgtgc tgccgatctc gcatatcgtc ggcatctcgc
     7321 tgctgatcat gaccctgatg gttggcggga cggtccgcct ggtcagcaaa tatgaccccg
     7381 cggcgctggc cggggctatc gccgaagagg gaatcacgat cctgaacggc gtccccgcca
     7441 cctatcagcg gctgctggaa tacaaggccg tgaccggcct tgcgcaactc gaccgtggat
     7501 cgctgcggct gatcgcggtt gccggcgctc cgcttgatct cgacctgaag tcgggcgttg
     7561 aacgagagtt cggtctgcca ctttccaacg ggtacggcat caccgagtgt tcgccgggaa
     7621 tatccggcgt gcgctttgat gcgccgcgcg ccgaccaggc ggttggaacg ctgctgccgg
     7681 gcatcgaggc tcgcatcgcc cggctcgatg gcggaatggt gccgaacgga gaaatcggtg
     7741 aacttcacgt ccgcggccgc aacgtgatgc gcggctatta tcgcgccccc gagctgacgg
     7801 caaaggccat tgataccgac ggttggttca acaccggtga tctcgcgcgc ttcgacggcg
     7861 attgcctcta catcgtcggg cgcagcaagg agatgatcat ccgctcaggc tttaacgtct
     7921 atcctgcgga ggtcgaggcc gtcctcaatt cgcatgccgc ggtggttcag tccgctgtgg
     7981 tcggccgcgc tgtcgaaggc aacgaggagg ttgtcgcttt cgtgcagctg atgcagggtt
     8041 cgcctgtcaa gccggccgag ctgatggctt ttatcaaccc gcagctgacc tcgtacaagc
     8101 ggccgtcgga gatcatcgtg ctcgatgcct tgccggcaac ctcgaccggc aagatcctca
     8161 agcacaagct cgcggagtct ctgcgcggcg cgtagcccgc gaaaaagatc gcgcggcgca
     8221 agttcgaagg cgacagagtt aacctgaccg ggagaacacc cttgcagaag agaaacgcga
     8281 ccgtggccgt gatcggcgcc ggggattaca tcggcggcga aatcgccaag aagtttgcct
     8341 tggaagggtt cacggtgttc gccgggcgcc gcaacggcgc caagctcgaa ccgctggtga
     8401 aggagatcga ggcggccggc ggcgaaatcc acgcgcgctc gctcgatgcg cgcaaggagg
     8461 aggagatcat ctccttcctc ggagacgccg acaggcatgc cccgctcgag gtctgcattt
     8521 tcaacgtcgg cgctaatgtc aatttcccga ttctcgatac gacggagcgc gtgttccgca
     8581 aggtctggga aatggcctgt tattccggct ttctcgccgg ccgtgaagcc gcgcgcctga
     8641 tgctgccccg cggccagggc aatatcttct tcaccggtgc gaccgcgagc cttcgcggcg
     8701 gagccggcta tgcggccttc gccagcgcca aatttggctt gcgggccgtc gcccaggcga
     8761 ccgcgcgcga gcttggcccg aagaacatcc atgttgccca tctcatcatc gactccggcg
     8821 tcgacaccga atgggtgcgt cagcggcggc tggaggcgct gggaccgggt gccctcgata
     8881 acccagatct cctgatgccg ccgtcatccg tcgcggaatc ctactggcag ctctatcagc
     8941 agccacgcag cgcctggacg ttcgagctgg aaatacgacc ctttggcgag aagtggtagc
     9001 gtgagccgcg ggcttcttga aatcgtccat tgatccaaag tgctgcaaac agacgggagc
     9061 cattcttgaa aaccgcgatc actgaaatgt tcggcatcga acatccgatt atccagggcg
     9121 gaatgcatta cgtcgggttt gcggaactcg cagccgcggt ctcgaacgcg ggtggccttg
     9181 gcatcattac cggcctgacg caaaggacgc ccgatctgct cgccagggaa atcgcgcgct
     9241 gccacgacat gacggacaag ccgttcggcg tcaacctgac ctttctgccg gcgttcagcg
     9301 cgccgcccta tccggaatac atcgcggcca tccgggaggg cgggatcaag atcgtcgaaa
     9361 ccgcaggacg cagccccgag cagtacatgc cggcgctgaa ggcggccggc atcaaggtga
     9421 tccacaaatg cacctcggtc cggcactcac tgaaggccga gcagatcggc tgcgacgcgg
     9481 tcagcgtcga tggcttcgaa tgcggcggac atcccggcga agacgacatg cccaacatga
     9541 tcctgctgcc gcgcgccgcc gaggaactga aaataccatt cgttgcctcg ggcggcatgg
     9601 ccgacgcgcg aagcctggtg gcagcactcg cgctcggcgc cgccggcatg aacatgggaa
     9661 cgcgctttat tgcgaccagg gaagcgccgg tgcacgagaa cgtcaagcag gcgctggtcg
     9721 ccgctaccga gctcgatacc aggctggtca tgcgcgcgct gcggaacacc gagcgtgtcc
     9781 tcaggaatgc cggcgtcgac cgtctgatcg agatcgaacg ggagaagggc aggagcctca
     9841 ggatcgagga catccatgac gaggtcgcgg gcgtctatcc aaaggtgatg atcgacggtg
     9901 acctggatgc cggcgcctgg agttgcggca tggtcgtagg cttgatcaag gacattccga
     9961 cggtgaagga actgatcgac cgcatcatgg cggatgcgga gcatatcatc cgtagccgcc
    10021 tgaccggctt tctcgatgat gggaaagcga cacggagggc ggcgtgaaag gccgatcctg
    10081 gccggcgcca aagtccgcgt cacgacgacc gcgggaagtg acaatccatc cataacgcag
    10141 ggaaataacc cgatgaccga ccacgttcgc cgcgaaatcg ccgatggcat catgacgttg
    10201 actatttccc gctccgacaa gaagaatgcg ctgagcaacg cgatgtacag cgcgctttcg
    10261 gacggactgg agcttgcgga acaggatccg tcggtcaggg tcgtgttgtt tcagggcgac
    10321 ggcgacagct ttacggctgg aaacgatctg gccgatttca gtgccgaggc gagcggcaaa
    10381 agcgtcggcg agagccaggc cgctcgcttt atcggcaacc tcgcgcgggc gaccgtgcca
    10441 ctcgttgccg cggtgcaagg caatgcggtc ggcgtcggca cgacgatgct gctgcattgc
    10501 gacctcgtct atcttgccga caacgcgaaa ctgataacgc cgttcgtcaa cctggcgctt
    10561 gtgccggaag cttcatcgag ctggctgttg ccggcccgga tcgggcatgc acgggcctat
    10621 gccatgtttg cactcggcga gccgatggat gcgtcttccg cgctggcttg cggactggcg
    10681 aatgccgtcg tgccgctgtc cgagctgcgg accaaggcgc atggcgccgc cgcggcattg
    10741 acgaaacggc ccgccggctc tctcaaactg accaaggcct tgatgcgcga aaccgaccgg
    10801 atcgttgccc agatcgcccg cgaaggcgcc ctgttcaggg atcggcttca atccggcgag
    10861 gcgcgggaag ccttcgcggc cttcgccgag aaacgtaagc cggacttttc gaaggtgtcc
    10921 ggctagggca gagtcccgat tcaattttca aacagcgcgt aaacccatag ccgtcattcc
    10981 ggggcgatgc gaagcatcga acccggaatc tcgagattcc ccgatgcgca attgcgcatc
    11041 tggtccttcg gaccatcccg gaatgacgga gcctggtcgc ttcgcttcgc tccacatacg
    11101 actccgccct cgcggcgcga tgcgcccgag tcgtgcgttt agctctcgcc cccttggagg
    11161 gcgtggggaa tgccgggtgc ccgctgcacc cgcggcctcg tgtgcacttt gtattggtag
    11221 aacgcacacg agtaacaacg agtacaccgg aacgcccggc attcccgcac gcaatggttt
    11281 taacggctta tgtcgtg
//
LOCUS       DATNOI010000078         5229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1184607,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000078 DATNOI010000000
VERSION     DATNOI010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184607"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1356
                     /locus_tag="VN968_02700"
     CDS             <1..1356
                     /locus_tag="VN968_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXL28192.1"
                     /translation="PAPPSAAPSAAPSTAPAPQLQQKNAPEQLQQKNAPAPVPPPPAG
                     TKQPPAAAPQTQTAPPPPPPAGAGTPPPPRTGPPPSAAPNAAPAPGAAGGQTSLPPGA
                     PAGAAPFVRHPPPAVSAPLPAAPPPAQALAPIAPGAPAPGPRRMDDFRGARRETQEGG
                     RTVFNEPGRVIVVDPGGQSFIRHNEEERFRFGARDIRTEQVGGESRTIVIRPDGSQII
                     TVTGRDGQLLRRIRRDERGREIIIIDNSYRDPSAVGGFYVDLPPPVIRIPYNRYIVDA
                     EDASPDLIYDTMEAPPVDRIERRYSLDEIRYSPSVRQLMPSIDLNSINFETGSWEIPP
                     DQAAKLQSIADGLNRAISRNPREVFLIEGHTDAVGSDVDNLSLSDRRAQAAAELLTQQ
                     FQVPAENLTSQGYGKQYLKEQTDGPSRINRRVTIRRITPLLNGGTASLPPPPPGVAPP
                     R"
     gene            complement(1697..3034)
                     /gene="argG"
                     /locus_tag="VN968_02705"
     CDS             complement(1697..3034)
                     /gene="argG"
                     /locus_tag="VN968_02705"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HXL28193.1"
                     /translation="MSTILKSLPKGEKVGIAFSGGLDTSAALLWMKQKGARVFAYTAN
                     LGQPDEADYNEIPRKALEYGAEKARLVDCRTQLVHEGIAAVQSGAFHVSTGGITYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTFKGNDIERFYRYGLLTNPSLRIYKPWLDQ
                     QFIDELGGRAEMSVFLTAHGFGYKMSAEKAYSTDSNILGATHEAKDLEQLDSGIKIVN
                     PIMGVPFWRDDRNIRAEKVVVRFAEGQPVALNGQTFADPVALFLEANAIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLITGIHNEDTIEQYRISGMRLGRLLYQG
                     RWFDSQALMLRETAQRWVARAVTGEVTLELRRGNDYSFLNTESPNLTYQPERLSMEKV
                     EDAAFTPADRIGQLTMRNLDIADTRAKLNLYSETGLLSSGEGSPIFKLDSDKS"
     gene            complement(3146..3922)
                     /locus_tag="VN968_02710"
     CDS             complement(3146..3922)
                     /locus_tag="VN968_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXL28194.1"
                     /translation="MNRSGLLIALALALVIGLLFGIYPELDLKLAALFYDGATRTFPL
                     KLNAMAALARDGAMWIAWAIAMPAIAALAVKLVRPDRPLLISGRAIIFLLVTLTLSAG
                     ILTNLTFKSYWGRPRPVVVTQFNGDLPFVPWWDPRGGCSRNCSFFSGEGATAFWTFAP
                     AALTPPAWRPLAYAAATLFGVATSALRMAFGGHFLTDVAAAGLVTFLVIWLAHGYIYR
                     WRQTRLSDESIDAALTRPSWPGYRLGQRWRGRDVGPLPPG"
     gene            complement(4090..4587)
                     /locus_tag="VN968_02715"
     CDS             complement(4090..4587)
                     /locus_tag="VN968_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28195.1"
                     /translation="MALIGRLFVILFGFLAACLVAGMIVVGAVLFPEFSDLATGPIDQ
                     SAVNVVLGFGFIFVSGFALLPAMIVVAITEAFYIRGVLTYAVGGGVVGLACYLGLIPF
                     DPATLSFDGIVRRHLEIMTGAGIVAGLVYWMIAGRNAGAWREPPRPLPPLPPLPSHSP
                     SPPPS"
     gene            complement(4711..>5229)
                     /locus_tag="VN968_02720"
     CDS             complement(4711..>5229)
                     /locus_tag="VN968_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXL28196.1"
                     /translation="RITLSTSGVVPNIIRTGDEIGVMLAISLHAVRDELRNELVPLNR
                     KYPIAELLQACRDYPGSSNARRITFEYVMLKGVNDSLEDAKLLVKMLKGIPAKINLIP
                     FNPWPGTRYECSDWDQIEKFSEYIFNAGYSSPVRTPRGRDILAACGQLKSETEKLSVR
                     ERQALRAMAMTD"
BASE COUNT         1001 a         1690 c         1642 g          896 t
ORIGIN      
        1 ccggcgccgc cgagcgctgc gccttcggct gcgccgagca cggcaccagc gccccagctt
       61 cagcaaaaaa atgcgccaga acagcttcag cagaaaaatg cgccggcacc ggtaccgcca
      121 ccgcccgccg gcacaaaaca gccgcctgca gccgctccgc agactcaaac tgcaccaccc
      181 ccgccaccac cggcgggagc gggtacgcca ccgccgccgc gtaccggtcc gcctccgagc
      241 gctgcaccga atgccgctcc cgcaccgggt gcggccggcg gacagacgtc gctgccgccc
      301 ggagcgcctg caggcgccgc gccatttgtc cgtcacccgc cgccagccgt cagcgcgccg
      361 cttccggccg ccccgccgcc ggcccaagcc ttggcgccga ttgctccggg tgcgccggcc
      421 cctgggccgc gccgcatgga tgacttcaga ggggcacgcc gggaaaccca ggagggcggc
      481 cgcaccgtct ttaacgagcc cggccgcgtc atcgttgtcg atcccggcgg ccaatccttc
      541 attcgccaca acgaagaaga gcgcttccgc ttcggcgcgc gcgacatccg caccgagcaa
      601 gtcggcggcg aatcgcgcac catcgtgatc cgtcccgacg gcagccagat catcaccgtg
      661 accggccgcg acggtcagtt gctgcgccgg attcgccggg atgagcgggg ccgcgagatc
      721 atcatcatcg acaattccta ccgcgatccc agcgcggtcg gcggtttcta cgtcgacctg
      781 ccgccaccgg tgatccgcat tccgtacaac cgctacatcg tcgacgccga ggacgcctcg
      841 cccgatctga tttacgacac catggaggcg ccgccggtgg accggatcga gcggcgttat
      901 tcgctcgacg aaatccgcta cagcccgtcg gtgcggcagt tgatgccgag catcgacctc
      961 aacagcatca acttcgaaac cggatcgtgg gaaattccgc ccgatcaggc ggcgaagctg
     1021 cagtcgatcg cggacggcct caaccgggcg atctcgcgca atccgcgcga ggtgttcctg
     1081 atcgagggcc ataccgatgc ggtgggatcg gacgttgaca atctgtcact gtcggatcgc
     1141 cgcgcccaag ccgccgccga attgctgact cagcaattcc aggtgccggc ggaaaacctg
     1201 acctcgcaag gatacggcaa gcagtatctg aaggaacaga ccgacgggcc gagccgcatc
     1261 aaccggcgcg tcaccatccg ccgcatcacg ccattgctca acggcggaac agcatcgctg
     1321 ccgccgccgc cacccggcgt cgcaccgccg aggtgaggtg aggcagctgc tagatcatga
     1381 tccgattaga ttgaatcgga tcatgatctc atctcttggt ttggacgtcg ttattccacg
     1441 agtcatcccg gcgaacgccg ggatccataa cccctggctt aggctgttga agaaggtatc
     1501 tgctccagtg ccgaagcgag actccgcacc gtatgggtcc cggcattcgc cgggacgacg
     1561 tggagacgtg caagtcccca cagccccagc ttcttctcgc gctggctgag cgtgcgcagc
     1621 cgcaaggcgt tgagcttgat gaagccggcg gcgacgcgat gatcgtaggc aaccgcacct
     1681 taaatgcccg cgcgtttcag ctcttgtcgc tgtcgagttt gaagatggga gagccttccc
     1741 cgctcgatag caagcccgtc tcagagtaga gattcagttt ggcgcgggta tcggcgatat
     1801 cgaggtttcg catggtcagc tggccgatac ggtcagctgg ggtgaacgcc gcatcctcca
     1861 ccttttccat gctcagccgc tccggctgat aggtcaggtt ggggctctcg gtgttcaaga
     1921 atgagtagtc gttgccgcgg cgcagctcga gggtcacctc gccggtgacc gcacgagcca
     1981 cccaacgctg ggccgtttct cgcagcatga gggcttgaga atcgaaccac cgcccctggt
     2041 agagcaatcg gccaaggcgc atgccgctga tccggtattg ctcgatggtg tcttcgttgt
     2101 ggatgccagt gatcagacgt tcgtacgcga tgtgcagcag cgccatgccg ggagcttcgt
     2161 agataccgcg gctctttgcc tcgatgatcc ggttctcgat ctggtcgctc atgccaaggc
     2221 catgtcgacc gccgatagcg ttagcctcaa ggaacagcgc gaccggatcg gcgaacgtct
     2281 ggccgttcag cgcgacgggc tggccctccg caaaacgcac gacgaccttt tcggccctga
     2341 tgttgcggtc gtcgcgccaa aacggcacgc ccatgatcgg attgacgatc ttgatgccgc
     2401 tgtccagctg ctcaagatcc ttggcctcgt gggttgcgcc gaggatgttg ctgtcggttg
     2461 aataagcctt ttcggcgctc atcttgtagc cgaagccatg ggccgtcaga aacaccgaca
     2521 tttccgcacg gccgcccagc tcgtcgatga actgctggtc gagccaaggc ttatagattc
     2581 tgaggctcgg gttggtcagc aggccgtaac ggtagaagcg ctcgatatcg ttgcctttga
     2641 aggtggaacc atcgccccag atgttgactc cgtcctcctt cattgccgcg accagcatcg
     2701 tgccggtcac ggcgcgaccg aggggcgtgg tgttgaagta cgtgatgccg ccggtgctga
     2761 cgtggaaggc accggattgg acggcggcaa taccttcgtg caccaattgc gtgcggcaat
     2821 ccacgagtcg ggctttttcg gcgccgtact ccagggcctt gcgcggaatc tcgttgtagt
     2881 cggcctcgtc aggttggccg aggttggcgg tataggcaaa gacgcgggcg cctttttgct
     2941 tcatccacag gagtgccgcg ctggtgtcga ggccgcccga gaaggcgatg ccgacttttt
     3001 cccccttggg caggcttttc aagatcgtac tcatagaatt tccaatcggt cgaaatttcc
     3061 aatgccgctc ttggcttgat tgcgcgaata tcaaatttcg cgccgctggg cacgcgttta
     3121 atagcggctg gcgttatgga ggcgctcacc ccggcggcag cgggcccaca tcgcggccgc
     3181 gccagcgctg tccgaggcgg tatcccggcc aggacggccg cgtcagtgcg gcatcgatgc
     3241 tctcgtcaga tagccgggtc tgacgccagc ggtagatgta gccatgcgcc agccagatca
     3301 ccagaaacgt caccagcccg gcggcggcga cgtcggtcag aaagtgcccg ccgaatgcca
     3361 tccgcaacgc gctggtggcg accccgaaca gggtcgcggc agcataggcc aacggccgcc
     3421 aggccggcgg cgtcagcgcg gcgggcgcaa aggtccagaa tgcggtggcg ccttcgccgg
     3481 agaaaaacga gcagttgcgg ctgcagccac cgcgcggatc ccaccacgga acgaaaggca
     3541 aatcgccgtt gaactgcgtc accaccaccg ggcgcggccg gccccaatag ctcttgaagg
     3601 tgagattggt gagaatgccg gcggacagcg tgagcgtaac caacaggaag atgattgcgc
     3661 gccccgatat caagagcggc cggtccggcc gtaccagctt caccgccaat gcggcgatcg
     3721 ccggcatcgc gatggcccac gcgatccaca tcgcgccgtc gcgcgcaagt gctgccatcg
     3781 cattgagctt cagcggaaag gttctggtcg ccccgtcata aaacagggcg gcaagtttga
     3841 gatcgagttc gggatagatc ccgaacagca gtccgatgac aagcgccagg gcgagcgcga
     3901 tgagaagtcc tgatcggttc atggcgcggg gtttagccga ggaggttgga gatgaaaacc
     3961 cccgtcatgg cgggccaacg ggtcgggcga atgcgcgccc gataaggggc tccgcaaggc
     4021 gatccatgag acgcgaggcc ggactggatt gcttcgtcgc ttgcgcttct cgcaatgacg
     4081 gcggagcaat cacgacggcg ggggggacgg tgaatgcgac ggcaacggcg gcagaggcgg
     4141 cagcgggcgc ggcggctcgc gccacgcgcc ggcattgcgg cccgcgatca tccagtagac
     4201 gagacccgcg acgattccgg caccggtcat gatctcgaga tgacgccgga cgatgccatc
     4261 gaaggaaagt gtcgcgggat cgaacgggat caggccgaga tagcacgcca ggccgaccac
     4321 gccaccgccg accgcgtagg tcagcacgcc cctgatatag aacgcttctg tgatcgcgac
     4381 cacgatcatc gccggcaaga gcgcaaagcc gctgacgaag ataaagccga agcccagcac
     4441 gacgttgacc gcgctctgat cgatcgggcc ggtcgccaga tcgctgaatt ccggaaacag
     4501 caccgctccg accacgatca ttccggccac caggcacgcg gccagaaatc cgaacaagat
     4561 cacgaacagc cggccgatca atgccatgcg gtcataccga aattgccgtc attgcgagcg
     4621 caagccaagc agtccaggag cgccgggcga ggctggattg cttcgtcgct tgcgctcctc
     4681 gcgatgacga gcttagaagt cgcgtcatcg ctaatccgtc atcgccatgg cgcgcagcgc
     4741 ctgccgctcg cgcaccgaca atttctcggt ctcggatttg agctggccgc aggcggcaag
     4801 gatatcgcgg ccgcgcggcg tgcgcaccgg cgaggagtag ccggcgttga aaatatattc
     4861 ggaaaacttt tcgatctggt cccagtccga gcactcgtag cgggtgcccg gccacggatt
     4921 gaacggaatg agattgatct tggccgggat ccccttcagc atcttgacca ggagcttggc
     4981 gtcctcgagc gaatcgttga cgcctttcag catcacatat tcgaaggtga tacgccgcgc
     5041 gttcgacgac cccggatagt cgcgacaggc ctgcagcaat tcggcgatcg gatatttgcg
     5101 gttcagcggc accagttcgt tgcgaagttc gtcgcgcacc gcatgcagcg agatcgccag
     5161 catgaccccg atttcatcgc cggtccggat gatgttcggc accacgcccg aggtcgacag
     5221 cgtgatgcg
//
LOCUS       DATNOI010000079        10785 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1784416,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000079 DATNOI010000000
VERSION     DATNOI010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10785
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784416"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VN968_02725"
     CDS             complement(<1..437)
                     /locus_tag="VN968_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL28197.1"
                     /translation="MAETPVTDLDEKPADKTALLEIRGLKTHFATDDGIVHAVDGVDL
                     TVDRGETVCVVGESGCGKTVTALSVLKLIAMPPGRIAGGQILWQGRDIVPLPADEMNR
                     IRAREIAIVFQEPMTSLNPVYTIGDQIGEVIALHEGLNRRAVL"
     gene            complement(441..1382)
                     /locus_tag="VN968_02730"
     CDS             complement(441..1382)
                     /locus_tag="VN968_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL28198.1"
                     /translation="MAGLTHASPTVDVAVPLEGRSRRISPGFEAWRKFRRHRMAVASV
                     VILTVLTSAVVLGPVIWRVPINEIDFTARLSVPTWQHPFGTDDLGQDLLARMLYGGRI
                     SLAVGFAAMSVAIVAGTTIGAISGISRGGVDAVLMWITDLFLALPQLPLLLLVIYLFR
                     DFLKNLVGPEGGVFILIVLVIGGFRWMPVARLVRAQFLSLREKEFVEAARALGATTPR
                     LVIRHILPNSLGPVIVAGTIDVAAAIIAESTLSFLGLGFPPDIPTWGRLLFDAKDYLD
                     IAPHWALFAGAAIFLTVLAINFIGDGLRDALDPRKVM"
     gene            complement(1348..2307)
                     /locus_tag="VN968_02735"
     CDS             complement(1348..2307)
                     /locus_tag="VN968_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL28199.1"
                     /translation="MGKYILRRLVIALPSLLGISLILFVLLALAPGDPFGELAANTNI
                     PPEVRAALRVKFGIDDPILIRYVRWLVAMLHGDWGFSFSSRINVDQLILQRVPTTLFV
                     VGSSQILALAIALPVGVYAAMRPYSIFDQIANTVAFVGFSLPTFFTGLLLILIFSVNL
                     GWLPFVYRTDIAATGWHWYWENFRQAIMPVAVLGLFQAASYTRYVRSAVLDVVRMDYV
                     TTARSKGLDEGKVIVKHVVRNALIPVVTLVALQMPAVFGGAIVTEQIFRIPGIGSLLI
                     TAILANDTPVIMAVTFVFACLVVVFNLLADIIYGWLDPRISYR"
     gene            complement(2364..4154)
                     /locus_tag="VN968_02740"
     CDS             complement(2364..4154)
                     /locus_tag="VN968_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL28200.1"
                     /translation="MKERELRDLIADVKTGRLSRRTFVQKMIAVGLTAPMAGMMLSHS
                     GVAMAATEIPYKPTKAGGGGPLKLLYWQAVTLLNPHFAVGTKDQEGSRIFYEPLAGWD
                     NEGNLVPFLAAEIPSKENDGLAEDGLSVVWKLKRGVKWHDGMPFTADDVVFNWEYART
                     PETAAVSVASYKDIKVEKIDDFTVRVSFAKPTPFWADAFVGSYGMIIPKHLFADYIGG
                     KSRDAPANLKPVGTGPYTFVDFKPGDMVRAKLNPDYHVPNRPHFDTVEIKGGGDAVSA
                     ARAVLQTGEYDYGYNMLVEDEILLKLESAGKGRVSIVETGAVEFIMLNATDPAVEVDG
                     ERASLKTKHPLFSDPAVRQAINLLIDRASIEKFIYGRTASATANFLNSPERVRSKNTK
                     LEFNIEKANAILEAAGWKKGADGVRAKDGKSLKFVYQTSINAPRQKAQAIVKQACQKA
                     GIEIELKSVVASVFFSSDTANPDTYTHFYCDAQMYNTTMTQADPQFFMNQHLSWQAAT
                     KENKWQGRNISRWQNKEYDEVYKQAEQELDPVKRAAMFIKLNDIVVADNYILPLVRRP
                     KVTALKSSLTAHISGWDNDLYQIASWYREA"
     gene            4698..5420
                     /locus_tag="VN968_02745"
     CDS             4698..5420
                     /locus_tag="VN968_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HXL28201.1"
                     /translation="MTRNSNMRDAILSAAEVLFSTSGFNAVSIRDIAHEAGANPGSVT
                     YHFKSKDGLLLEIYKRHCGPMNRRRIELLVAAMRVRDLQNRLEAVVRAYVQPAFSSSS
                     DLAGGGARFTRLRAVMSAEGNPVVGRIIAEIFDDTTHAFIDAIQQSLPHLPRTTIVWR
                     GQFLLGALYYALVNPERVSRLSRGEADGSDMAEGIEQLVSSTVASLQAPDPNDLRGET
                     TVGPRKAPQRAKLAKAKSAVRS"
     gene            5450..6379
                     /locus_tag="VN968_02750"
     CDS             5450..6379
                     /locus_tag="VN968_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL28202.1"
                     /translation="MLRRKFLKGAAGFGAISALGGNPSHGAEGSAAPTRSIPEIAGPD
                     KNTKTPAFKMPAGTIDTHCHIFGPAAEYPFSPTRPYTPPEAPLSMFRALHEKIGVERA
                     VIVNATMHGFDNRVVTDAIARSDGKYRGVANINGAMSDADLLSLNKAGMRGCRFAFLK
                     RLGGVGDMNAFKRLVDRAAGIGWHVDVYLEPGSIREFTPILTAMPTTYVIDHMGTIDA
                     AKGLGTAALHQSVVEGAVGPGDDRHGARRGAWNLQARLRHSDAAFRRPPAMGHRRTLP
                     VNCSTTWPGSRSWPFHTRAERLHSRRCWPEIYP"
     gene            6295..6747
                     /locus_tag="VN968_02755"
     CDS             6295..6747
                     /locus_tag="VN968_02755"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL28203.1"
                     /translation="MAGVKILAIPYKGGAPTLTAVLAGDIPMSINPMPEVVGQLDSGL
                     VRVIAVTTATRSKALPDVATVAESGVPGYETSVWWGFLGPAGMAPELVAKINADLLSV
                     LKDPAVLAAFDKPGAVPVGSSPQDFEAFMHAEAAKWGPILKQANVRIE"
     gene            6890..7918
                     /locus_tag="VN968_02760"
     CDS             6890..7918
                     /locus_tag="VN968_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL28204.1"
                     /translation="MIIDVHGHYTTAPKALEDWRNRQIAGIKDPSARPEVSELKISDD
                     ELRESIINNQLKKMEERGSDLTIFSPRASFMAHHIGDFEVSSTWATICNELCARVSQL
                     FPDHFIGAAMLPQSPGVDPKTCIPELEKCVEDYGFVGINLNPDPSGGHWTSPPLSDRQ
                     WYPIYEKMVELDIPAMIHVSTSCNACFHTTGAHYLNADTTAFMQCLTSDLFKDFPTLR
                     FLIPHGGGAVPYHWGRFRGLAQELKKPLLKDHLLHNIFFDTCVYHQPGIDLLTKVIPV
                     ENILFASEMIGAVRGIDPETGFPYDDTKRYIEASTILSAQDKHKVYEGNARRVFPRLD
                     AALKAKGK"
     gene            7920..8615
                     /gene="ligK"
                     /locus_tag="VN968_02765"
     CDS             7920..8615
                     /gene="ligK"
                     /locus_tag="VN968_02765"
                     /EC_number="4.1.1.112"
                     /EC_number="4.1.3.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HXL28205.1"
                     /translation="MTGPINNLGIVKRNIVRADMAAVEKLSRFGVATIHEAMGRVGLM
                     HPYMRPIYAGAQACGTGVTVLLQPGDNWMMHVAAEQLQPGDVVVAACTAENTDGFFGD
                     LLATSFHARGAKGLIIDGGVRDVKDLTDIQFPVFSRAISAKGTVKATLGSVNIPVVCA
                     GALVNPGDVVIADADGVVVVPAGIAPQVADAAEAREANEGEKRARLAAGVLGLDMYNM
                     REPLAKAGLRYID"
     gene            8621..9544
                     /locus_tag="VN968_02770"
     CDS             8621..9544
                     /locus_tag="VN968_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HXL28206.1"
                     /translation="MSTNSNQWHVGLVGYGEVGRILAEDLRKQDIRVSAYDIELGGDQ
                     AGPLRDHAGAFGVALASSHADLAAQADFIVSAVTASQAVPVAQACASAIKKGAWFLDF
                     NSASPGAKRRAAALIDGAGGRYVEGAVMTSVPPYRIKVPLLLGGSGARELAPMLVELG
                     FEAKVASEELGVASAVKMCRSVMIKGFEAMVIECFTTARAYGVEDAVLASLAETFPGI
                     NWEKQGAYFFQRVIEHGRRRSEEVREVAETVREIGLTPWSAQGTAERQAWVADLADEG
                     LFGPKGTKEFARSPDWRTEADRILARLNRGE"
     gene            9556..10479
                     /locus_tag="VN968_02775"
     CDS             9556..10479
                     /locus_tag="VN968_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL28207.1"
                     /translation="MEFQKTPGWLDWYAGPSKPRFKLPAGAVDAHCHVFGPGAEFPYA
                     PERKYTPCDASKQQLYALRDHLGFARNVVVQATCHGADNRAMVDALLDSGGRARGVAT
                     VRRSVGDAELNAMHAAGVRGVRFNFVKRLVDFTPKEELIEIAGRIGKLGWHVVIYFEA
                     MDLPELWDFFTSLPTAIVVDHMGRPDVSRPADGPEFELFMKFMRDHANVWSKVSCPER
                     LSQSGPPALNGEQNAYRDMIPFARRIVETFPDRVLWGTDWPHPNLKNHMPDDGLLVDF
                     IPHIATTPDLQRKLLVDNPMRLYWPEEATGR"
     gene            complement(10569..>10785)
                     /locus_tag="VN968_02780"
     CDS             complement(10569..>10785)
                     /locus_tag="VN968_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HXL28208.1"
                     /translation="AARVKICLAQARLSASDIDAVFLTGGSVRLAHVRKAITKAVPSA
                     HIVEGDTFGAVGKGLTLEALRRYGPRD"
BASE COUNT         2128 a         3313 c         3357 g         1987 t
ORIGIN      
        1 tccagcacgg cgcgacggtt caatccttca tgcagggcga tgacctcgcc gatctgatcg
       61 ccgatggtat agaccggatt gagcgaggtc atcggctcct ggaacacgat ggcgatttcc
      121 ctggcgcgaa tgcggttcat ctcgtccgcc ggcagcggaa cgatgtcgcg gccttgccac
      181 aggatctgcc cgccggcgat gcgcccgggc ggcatcgcga tcagcttcag gaccgagagc
      241 gcggtgacgg tcttgccgca gccggactcg ccgacgacgc aaaccgtctc gccgcggtcg
      301 actgtcagat cgacgccatc caccgcatgc acgatgccgt cgtcggttgc gaaatgagtc
      361 ttgagtcctc tgatctcgag cagcgcggtt ttatcagcgg gtttctcgtc gaggtccgtc
      421 acaggcgtct cagccaacat ctacatgact ttcctgggat caagcgcgtc gcgcaacccg
      481 tcgccgatga aattgatggc cagcacggtc aggaaaatgg cggcgccggc gaacagcgcc
      541 caatgcggtg cgatgtccag ataatccttg gcatcgaaca acagacggcc ccaggtcgga
      601 atatccggcg gaaagccgag gccgaggaac gacagcgtcg attccgcgat gattgcggcc
      661 gcgacgtcga tggtgccggc cacgatgacc ggtccgagtg aattgggcag gatatgccgg
      721 atcaccagcc gcggcgttgt cgcgccgagg gcgcgggccg cttcgacgaa ttccttctcg
      781 cgcaacgaca ggaactgggc ccgcaccagg cgcgccaccg gcatccagcg aaagccgccg
      841 atcaccagga cgatcagaat gaagacgccg ccttccggcc cgaccagatt tttgaggaaa
      901 tcccggaaca gatagattac cagcaacagc agcggtaatt gcggcagcgc cagaaacagg
      961 tcggtgatcc acatcaacac ggcgtcgacg ccgccgcggg aaatgcctga tatcgcgccg
     1021 atcgtggtgc cggcgacgat cgcgacggac atcgccgcga aaccgaccgc gagcgaaata
     1081 cgtccgccat acagcatgcg tgccagcagg tcctgaccga gatcgtcggt gccgaacggg
     1141 tgctgccatg tcggcacgct gagccgcgcg gtaaaatcga tttcgttgat cggcacgcgc
     1201 cagataaccg gcccgagcac aacggccgac gtcagcaccg tcaatatcac cacgctcgca
     1261 acagccatgc ggtggcggcg aaactttcgc cacgcctcga agccgggcga gatgcgtcgc
     1321 gaccggccct cgagcgggac ggcaacgtca acggtaggag atgcgtgggt caagccagcc
     1381 atagatgata tcagccaaaa ggttgaagac aacgacgaga caggcgaaca cgaacgtcac
     1441 cgccatgatc acaggggtat cattggcgag gatcgccgtg atcagcagcg agccgatgcc
     1501 ggggatgcgg aagatctgtt cggtgacgat ggcgccgccg aacacggcgg gcatctgcag
     1561 cgcgaccagc gtcaccacgg gaatcaaggc attgcgaacc acgtgcttga cgatgacctt
     1621 gccttcgtcg aggcccttcg agcgcgcggt ggtgacataa tccatgcgga cgacatcaag
     1681 caccgcggag cgcacgtagc gggtatagga cgccgcctga aaaagcccga gcaccgcgac
     1741 cggcatgatg gcttgcctga aattctccca ataccagtgc catccggtgg cggcgatatc
     1801 ggtgcggtag acgaagggca gccagccgag attgacgctg aagatcagga tcagcaacaa
     1861 ccccgtgaag aaagtcggca acgaaaagcc gacaaaggca acggtgttgg cgatctggtc
     1921 gaagatcgaa tagggccgca tcgcggcata gacgccgacc gggagcgcga tcgccaacgc
     1981 caggatctgc gacgagccga ccacaaacaa cgtggtcggg acgcgctgca ggatgagctg
     2041 gtcgacattg atgcggcttg agaacgaaaa cccccagtcg ccatgcagca tcgcgacgag
     2101 ccaacgcacg tagcgaatga gaataggatc gtcgatcccg aatttcaccc gtagcgcggc
     2161 gcggacctcg ggtggaatat tggtgttggc ggcgagctcg ccgaacggat ctcccggcgc
     2221 cagcgcgagc aggacaaaaa gaatgagact gatgccgagc agactgggaa gagcgatgac
     2281 caggcgccgg agaatgtatt ttcccatcgt cgtccagcca tcgcccgttc agtgtttgcg
     2341 aatcgcaaat ctcacatctg atctcatgct tcccgatacc agctcgcgat ctggtacagg
     2401 tcattatccc agccgctgat atgcgcggtc aggctgctct tcagcgcggt gacctttggc
     2461 cggcggacca gcggcaggat gtaattgtcg gcgacgacga tgtcgttgag cttgatgaac
     2521 atcgccgccc gcttcaccgg atcgagttcc tgttcggcct gtttgtaaac ctcgtcatat
     2581 tccttgtttt gccagcgaga gatgttgcgc ccctgccact tgttttcctt ggtggcggcc
     2641 tgccacgata ggtgctggtt catgaagaac tgcgggtctg cttgcgtcat ggtggtgttg
     2701 tacatctgcg cgtcgcaata gaaatgcgta taagtatccg ggttggcggt gtcggaggag
     2761 aaaaacaccg atgccacgac cgacttcagt tcgatctcga tacccgcctt ctggcacgcc
     2821 tgcttgacga tggcctgcgc cttctgccgg ggcgcgttga tcgaggtctg gtacacgaac
     2881 ttcagcgatt tgccgtcctt ggcgcgaacg ccgtcggcgc cctttttcca cccggccgcc
     2941 tcgaggatcg cgttggcctt ctcgatgttg aattcgagtt tggtgttttt cgaccggacg
     3001 cgttccgggc tgttgaggaa gtttgcggtg gcggaagcgg tacggccgta gatgaatttc
     3061 tcgatcgagg cgcgatcgat caagaggttg atggcctggc gcaccgccgg gtcgctgaac
     3121 agtggatgtt tggtcttcag gctcgcacgc tcgccgtcga cctcgaccgc cggatcggtt
     3181 gcgttgagca tgatgaactc gacagcgccg gtctcaacaa tgcttaccct gcctttgccg
     3241 gcactttcca gcttgagcag gatttcgtct tccaccagca tgttatagcc gtaatcatat
     3301 tcgccggtct gcagcaccgc gcgcgccgcc gacaccgcgt cgccgccgcc cttgatttcc
     3361 accgtatcga aatgcggccg gttggggacg tggtagtccg gattgagctt ggcacggacc
     3421 atgtcacccg gcttgaaatc gacgaacgtg taagggccgg tgcccaccgg cttcaaattg
     3481 gccggtgcgt cgcgcgattt gccgccgatg taatccgcga acaaatgttt cgggatgatc
     3541 atgccgtacg agccgacaaa ggcatcggcc cagaacggcg tcggctttgc aaagctcacg
     3601 cgaacggtga aatcgtcgat tttttcgacc ttgatatcct tgtagctggc gaccgagact
     3661 gccgcggtct cgggcgttcg cgcatattcc cagttgaaca cgacatcgtc cgccgtgaac
     3721 ggcatgccgt catgccattt gacgccccgc ttcaacttcc agacaaccga aaggccgtct
     3781 tcagcgagtc cgtcattctc cttgctcgga atctcggcgg ccaggaacgg aaccagattg
     3841 ccttcattgt cccatcccgc cagcggctcg tagaagatgc gggagccctc ctggtccttg
     3901 gttccaacgg cgaaatgcgg gttgagcagg gtcaccgcct gccaatagag aagtttcagc
     3961 gggccgccgc cgccggcctt ggtcggcttg tacgggatct ctgtcgcggc catcgctacc
     4021 ccggaatggc tcagcatcat gccggccatc ggcgccgtca ggccgacggc gatcattttt
     4081 tggacaaacg tccgccgcga caagcggccg gtcttgacgt ccgcaatcag atctcgaagt
     4141 tcccgttctt tcatggttcg gctcctcgct ggtgctgaat gctcaattca cccctcgcgg
     4201 ctcctgccac cgcagatgcc cagcgattgg aaatggatca ggtcaggacc accctgtcaa
     4261 agcaggatcc gggccaacac cacgtcaacg agtagcagca ctgagtgcga aaattgtcgg
     4321 tcgatagaaa agcctgtcaa ctcagaaccg catttcgctg gttgggcggc gaatgcggcg
     4381 ttcttgccgg cctgcagtgc acctgtcggt tgtgcgtcgt ttttggaagt caccaatttg
     4441 ctcgatcatg gcattggaac tgcacgcatg gaggccggct cagggcgcag ctactcgtcc
     4501 acgcaccccg ctcgggcagc gccgtaaaag cgtcgcgcgg gagttgcgca ggccctcgcc
     4561 tgagccgagg ctgtctggat tttgtgcctg cgaacacagg agagactgcc gcggcgcgca
     4621 acacggccct gcgtttcttt gttgacagga ccagttcctt tgtacattcg tacaaatgaa
     4681 aaacagagtc aaatccgatg acccgaaact cgaatatgcg ggatgccatc ctcagcgcgg
     4741 ctgaggtgct gttttccacg agcggtttca atgccgtttc aattcgcgat attgcgcacg
     4801 aggccggtgc caatcccggc agcgtcactt accacttcaa aagcaaggac ggcctgcttt
     4861 tggagatcta caagcgccat tgcggaccga tgaaccggcg gcgaattgag ctgctggtgg
     4921 cggccatgcg cgtgcgtgat ttgcagaacc ggcttgaggc ggtcgtgcgc gcatatgttc
     4981 agccggcgtt ttcctccagc agcgatcttg cgggcggcgg cgcgcgcttc acgcgcctgc
     5041 gcgcggtgat gtcggcggag ggcaatccgg tcgtggggcg catcatcgcg gaaatctttg
     5101 atgacacgac gcatgcgttc atcgatgcca tccagcaaag tctgccgcat ctgccccgta
     5161 caaccatcgt ttggcgaggt cagttcttgc tgggagcgtt gtactacgcc ctcgtcaatc
     5221 cagagcgcgt ctcgcgcctt tcgcgcgggg aggccgatgg ctcggacatg gctgaaggca
     5281 tcgaacaact cgtttcttct accgttgcct cgctgcaggc gcccgacccg aacgatctgc
     5341 ggggcgagac gaccgtgggt ccgcgcaagg ctccgcagcg agccaaactt gccaaggcca
     5401 agtcagccgt ccgcagctaa ttcccgttgg actcttggag catagcgaca tgcttagacg
     5461 gaagtttctc aagggtgccg caggcttcgg tgcgatatcg gcgctcggcg gcaatccgtc
     5521 acacggagcg gaaggctcgg ccgcgccaac cagatcaatt cctgaaattg ccggtccgga
     5581 caaaaatacg aaaacgcccg ccttcaagat gcccgcgggc acgatcgaca ctcattgtca
     5641 cattttcggt cccgccgccg aatatccatt ctctccgacg cgtccgtaca cgcctcccga
     5701 ggcgccgctg tcaatgtttc gcgcgctgca cgaaaaaatc ggtgtcgaac gtgccgtcat
     5761 cgtcaatgca accatgcacg gttttgataa tcgcgtcgtt accgacgcca tcgcccggag
     5821 cgatggaaaa taccgaggtg tcgcgaatat caacggcgcg atgtcggatg cagatctttt
     5881 gtccttgaac aaggccggaa tgcggggctg tcgctttgcc ttcctcaaga ggctgggcgg
     5941 tgttggcgac atgaacgcat tcaagcggct ggtggatcga gccgcaggca tcggttggca
     6001 tgtcgatgtg tatcttgagc ctggaagcat acgcgagttc acaccgattc taacggcgat
     6061 gccgaccacc tatgtgatcg atcacatggg cacgatcgac gccgccaagg gcctcggcac
     6121 cgcagcgctc catcaaagcg tggtggaagg agctgtgggt ccaggtgatg atcgccatgg
     6181 cgctcggcgt ggcgcttgga atctacaggc ccgacttcgc cattcagatg cagcctttcg
     6241 gcgacctccg gcaatgggac atcggcgcac cttgccggtg aactgctcaa ctacatggcc
     6301 ggggtcaaga tcctggccat tccatacaag ggcggagcgc ctacactcac ggcggtgttg
     6361 gccggagata tacccatgag catcaatcca atgccggaag tcgttggcca gctcgacagc
     6421 ggcctggttc gcgtgattgc agtgaccacc gccacccgat cgaaggcgct gccggatgtg
     6481 gcgacggtgg cggaatccgg tgtgcctggc tacgagactt cggtgtggtg gggatttctc
     6541 ggaccagcgg gcatggcgcc ggaactggtg gcgaaaatca acgccgatct tctgagtgtc
     6601 ctcaaggatc ccgcggtttt ggcggcgttt gacaagccgg gcgccgtgcc ggtgggaagt
     6661 tcgccacagg acttcgaggc attcatgcac gcggaggccg ccaaatgggg cccgatcctc
     6721 aagcaggcca acgtcaggat cgagtaatgt caggatcgaa tagcgactgg gcgcagttgt
     6781 cgatattgcg ctcgcgcggc gcgtatagaa attgatagtt tgaaaaacgc aaggaagcag
     6841 cgatgcaaaa tatcaaccgc acctgccgcc atccgaggag gagagccaag tgatcatcga
     6901 cgtgcatggc cactacacca cggctccgaa ggcgcttgag gattggcgca accggcagat
     6961 tgccggcatc aaggatccct cggccaggcc tgaggtcagc gagctgaaga tcagcgacga
     7021 cgaactgcgc gagagcatca tcaacaacca gctcaagaag atggaggagc gcggcagcga
     7081 tctcacgata ttcagcccgc gcgccagctt catggcccac cacatcggcg acttcgaggt
     7141 gtcgtcgacc tgggccacga tctgcaacga gctgtgcgcg cgtgtcagcc agctgtttcc
     7201 cgaccatttc atcggcgccg cgatgctgcc gcagtcgccg ggcgtcgatc cgaaaacgtg
     7261 catccccgaa ctggagaaat gcgtcgagga ttacggcttc gtcggcatca acctgaaccc
     7321 cgacccttcg ggcgggcact ggacctcgcc gccgctgtcg gaccggcagt ggtacccgat
     7381 ctacgagaag atggtggagc tcgacatccc cgccatgatc cacgtctcca ccagctgcaa
     7441 cgcctgcttt cacaccacgg gtgcgcatta cctcaacgcc gacaccaccg ccttcatgca
     7501 atgcctgacc tcggacctgt tcaaggactt tccgaccctg aggttcctga tcccgcatgg
     7561 cggcggcgcg gtgccctatc actggggccg cttccgcggc ctggcgcagg agctgaagaa
     7621 gccgctgctg aaagaccacc tgctgcacaa tatcttcttc gacacctgcg tttaccacca
     7681 gccgggcatt gaccttttga cgaaggtgat cccggtcgag aacatcctgt tcgccagcga
     7741 gatgatcggc gcggtgcggg ggatcgatcc tgaaaccggc ttcccctacg acgacaccaa
     7801 acgttacatc gaggcctcga cgatcctcag tgcccaggac aagcacaagg tctacgaggg
     7861 caacgcacgg cgcgtgttcc cgcgcctcga cgcagctctg aaagcgaagg ggaagtaaca
     7921 tgaccggccc gatcaacaat ctcggcatcg tcaagcgcaa catcgtccgc gcggacatgg
     7981 cggcggtcga aaagctgtcg cgcttcggcg tcgccaccat ccatgaggcg atgggccgcg
     8041 tcggcctgat gcatccctat atgcggccga tctacgccgg cgcgcaggcc tgcggaaccg
     8101 gcgtcaccgt gctgctgcag ccgggcgaca actggatgat gcatgtggca gcggagcagt
     8161 tgcagccggg cgatgtcgtg gtcgcggcct gcaccgccga gaataccgac ggcttcttcg
     8221 gcgatttgct tgcgacgagc ttccacgccc gcggtgcgaa gggcctgatc atcgatggcg
     8281 gggtgcgtga cgtgaaggac ctgaccgaca tccagttccc ggtgttttcc agggcgatca
     8341 gcgccaaggg cacggtgaag gcaaccctgg gctcggtgaa catcccggtg gtctgcgccg
     8401 gcgcgctggt caatccgggg gatgtggtga tcgccgacgc ggacggcgtc gtcgtcgttc
     8461 ccgccgggat agccccacag gtggccgatg cggcggaggc gcgcgaggcg aatgaaggcg
     8521 agaagcgcgc caggcttgca gccggtgtgc ttggcctcga catgtacaac atgcgcgagc
     8581 cgctcgcgaa ggctggcctc agatatatcg attgaccgcc atgtccacca actcaaacca
     8641 atggcatgtg ggactcgtcg gctacggcga agtaggccgc atccttgccg aagacctgcg
     8701 caagcaggac atcagggtca gcgcctatga catcgagctc ggcggtgatc aagccgggcc
     8761 cctgcgagat catgcgggag cattcggcgt cgcgctggct tcctcgcacg ccgatctcgc
     8821 cgcgcaggcc gacttcatcg tctcggccgt cacggcgagc caggccgtgc cggtggcgca
     8881 ggcctgcgcg tcggccatca agaaaggcgc gtggttcctg gacttcaatt cggcttcgcc
     8941 gggcgcaaaa cggcgtgccg cggccctgat cgatggcgct ggtggccgct atgtcgaagg
     9001 cgcggtgatg acatcggttc cgccataccg catcaaggtg cccttgctgc tcggcggcag
     9061 cggcgcccgg gagcttgcac cgatgctggt cgaactcggc ttcgaggcca aggtcgcgag
     9121 cgaagaactc ggcgtcgcct cggccgtcaa gatgtgccgc agcgtgatga tcaagggttt
     9181 tgaagcgatg gtcatcgagt gtttcacgac cgcgcgggcc tacggtgtcg aggacgcggt
     9241 gctggcttcg ttggcggaga cttttccggg catcaattgg gagaagcaag gcgcctattt
     9301 tttccagcgc gtcatcgaac atggcaggcg gcgcagcgag gaagtgcgcg aggtcgcgga
     9361 aaccgtgcgc gaaatcggtc tcacgccctg gtcggcgcaa ggcaccgcgg agcgtcaggc
     9421 ctgggtcgcc gatctcgccg acgagggcct gttcggtccc aagggcacca aggaatttgc
     9481 ccgcagcccc gactggcgca ccgaggcgga ccggatcctc gccaggctca accgcgggga
     9541 atgaaagaca aggcgatgga attccagaag acccccggct ggctggactg gtacgccggc
     9601 ccctccaagc cccggttcaa actgccggcc ggcgcagtcg acgcgcactg tcatgtgttc
     9661 ggcccggggg cggagtttcc atacgcgccg gagcgcaaat acacgccgtg tgatgcatcg
     9721 aagcagcaat tgtatgcgct gcgcgaccat ctcggctttg cccgcaacgt cgtggtccag
     9781 gccacctgtc acggcgccga caaccgcgcg atggtcgacg ccctccttga ttccggcggc
     9841 agggcgcgcg gcgtcgctac cgtcaggcgc agcgtcggcg atgcggaact caacgcgatg
     9901 catgccgccg gcgtgcgcgg cgtgcgcttc aacttcgtaa agcgtctggt cgatttcacg
     9961 ccgaaggagg aattgatcga gattgccggc cgtatcggca agctgggctg gcacgttgtg
    10021 atctactttg aagcaatgga tctgcccgaa ttgtgggact tcttcacctc gctgccgacc
    10081 gcgatcgtcg tcgaccacat gggcaggccc gacgtcagca ggcctgcgga tggtcccgag
    10141 ttcgagctgt tcatgaaatt catgcgggat cacgcaaacg tctggtccaa ggtcagttgc
    10201 ccggaacggc tgtcgcagtc gggcccgccc gcgttgaacg gcgagcagaa cgcctaccgt
    10261 gacatgatcc cgttcgccag acgcatcgtc gagactttcc ccgatcgtgt gctctggggc
    10321 accgactggc cgcatccgaa tctgaagaac cacatgccgg acgacggcct gctggtcgat
    10381 tttattccgc atatcgcgac cacgccagac ctgcaacgca agctgctggt cgacaatccg
    10441 atgcggcttt actggccgga ggaagcaacc ggtcgctaaa agtgccgatc ccgacaattt
    10501 cggccagcgc ggaagccggt tgaagcaagc ccgcgacaaa tcgcgttccg cgaagatcgc
    10561 cgccgccctc aatccctcgg gccataccgt cgcagcgcct ccagcgtgag gcccttgccg
    10621 accgcgccaa acgtgtcgcc ttcgacaatg tgggccgacg gcaccgcctt ggtgatcgcc
    10681 ttgcggacat gggcaagccg caccgaccct ccggtcagga acaccgcgtc gatgtcggat
    10741 gcggacaatc gcgcttgcgc gaggcagatc ttgacgcggg ccgcg
//
LOCUS       DATNOI010000080         2086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1201793,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000080 DATNOI010000000
VERSION     DATNOI010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201793"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VN968_02785"
     CDS             <1..1033
                     /locus_tag="VN968_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXL28209.1"
                     /translation="GLIQTIDVGLARTLFSWTLHAIVYFWLIPAYIAFYTMVPRAAGG
                     RLYSDTLGRLTFILFLVYSLPVGMHHLLMDPEHGNGTKFIQVLLTAFVSVPTLLTIFT
                     ITASLEIAGRLRGGRGLFGWIAALPWERPMVLATGLSFVMLGFGGFGGLINMGYGMNA
                     MVHNTSWVTAHFHLIFGGSVVIMYFAIAYEIWPRLTGHEYPSLKPLRLQLWLWFIGMM
                     VMTVPWHWLGLQGQWRRVANFNYADPIIAGWGPWVIVSLAGGLVLLASALLFIGNLFA
                     LHRSAAIAAARPLYALAVHPPQRVPAALNGFGLWNVLVLVLMLVAYGWPIAQFAIEPS
                     PAAIVHRVQ"
     gene            1044..1874
                     /locus_tag="VN968_02790"
     CDS             1044..1874
                     /locus_tag="VN968_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXL28210.1"
                     /translation="MSDPRISDPHVLDQPWRIWASIVVIGILLFGVALGVLIIPVVQG
                     RSAGIDMYTAICRALGILPGSPAQPQPATVSPPTPVSKVVWTPEVLQILARGNTERGR
                     AKVLEVCVACHGEQGVSVVPDYPNLNGQSGAAIYKQLHDYRTGSRANALMTGIANALE
                     EPTIADVAAYYAGQPKRNPNPASLGELPEAIVRLVELGDPNRNIPPCAACHRPGSGGP
                     IETPILSEQGRDYLAQQLRMYASGERRNDLYARMRVIAAKLTPGEIDGLSRYYSAGFR
                     "
     gene            complement(1885..>2086)
                     /locus_tag="VN968_02795"
     CDS             complement(1885..>2086)
                     /locus_tag="VN968_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28211.1"
                     /translation="LVVLMTIQGIGYLLPTNFRVCLELQKDNPDNARIGALTNRYFFA
                     VALQGVMQIVTIIVMARLATGV"
BASE COUNT          337 a          679 c          633 g          437 t
ORIGIN      
        1 cggcttgatc cagaccatcg acgtcgggct cgcacgcacg ctgttctcgt ggacgcttca
       61 cgccatcgtc tatttctggc tgatccccgc ctatatcgcc ttctatacga tggtgccgcg
      121 ggctgccggc ggtcggcttt acagcgacac cctgggccgc ctcaccttca tcctgttcct
      181 cgtctacagc ctgccggtcg ggatgcatca cctgctgatg gaccccgagc acggcaacgg
      241 caccaagttc atccaggtgc tgctcacggc gttcgtttcg gtgccgacct tgttgacgat
      301 tttcaccatc acggcgtcgc tggaaatcgc cggccgcttg cggggcgggc gcggattgtt
      361 tggatggatc gccgcgctgc cgtgggaacg tccgatggtg ctcgcgaccg ggctctcgtt
      421 cgtcatgctg ggtttcggcg gtttcggcgg gctgatcaac atgggctacg gcatgaacgc
      481 gatggtgcac aacacctcat gggtcaccgc gcatttccac ctgatcttcg gcggctcggt
      541 cgtaatcatg tatttcgcga tcgcctacga gatttggccg cgcctgaccg ggcacgaata
      601 tccttcgctc aagcctttgc gccttcaatt gtggctgtgg ttcatcggca tgatggtgat
      661 gaccgtgcca tggcattggc tcggattgca gggccagtgg cggcgggtcg ccaatttcaa
      721 ctacgccgat ccgatcatcg cgggttgggg cccatgggtg atcgtgtcat tggccggcgg
      781 gctcgttctg ctcgcctccg cgctgctgtt catcggcaat cttttcgcct tgcatcgaag
      841 cgcggcgatc gccgcggcac ggccgctcta cgcgctcgcc gttcaccctc cgcaacgcgt
      901 cccggcggcg ctcaacggct tcggtctttg gaacgtgctc gtgctggtgt tgatgctggt
      961 ggcctacggc tggccgatcg cccagttcgc gatcgagccc tcgcccgcgg ccatcgtaca
     1021 tcgcgtgcaa taggtccggc gtcatgtccg atccgcgcat ttccgatccc catgttctcg
     1081 atcagccctg gcggatttgg gcgagcatcg tcgtgatcgg catcctgctg ttcggggtcg
     1141 cactcggcgt tctgatcatt cctgtcgtgc agggccgcag tgcggggatc gacatgtaca
     1201 ctgccatatg ccgtgccctc ggaatcttgc caggctcgcc ggcccagccg cagcccgcga
     1261 ccgtctcgcc tccaactccg gtctcgaagg tcgtctggac gccggaggtt ttgcagattc
     1321 tcgcgcgcgg caataccgaa cgcgggcgcg ccaaggtgct ggaagtgtgc gtcgcctgcc
     1381 atggcgagca gggggtatcg gtcgtaccgg actatcctaa tctcaacggg caatcgggag
     1441 ctgcgatcta taagcagctg cacgactatc ggaccggcag ccgcgccaat gcgctgatga
     1501 ccggcatcgc gaatgccctg gaagagccga cgattgccga tgtggcggcc tattacgccg
     1561 ggcagccgaa acgcaatccg aatccggcaa gcctcggcga gttgccggag gccatcgtgc
     1621 gcctggtcga gctcggcgat cccaatcgca acatcccgcc gtgcgcggcc tgccaccggc
     1681 cgggatcggg cggccccatc gaaacgccga tcctgtccga gcaaggccgt gattacctgg
     1741 cgcaacagtt gcggatgtac gcctccggtg aacgccgcaa cgacctctat gcgcggatgc
     1801 gagtcatcgc ggctaaactg acccccggcg agatcgacgg actttcccgc tattacagcg
     1861 ctggctttcg ctgaaagcgc cgcctcagac tcccgttgcc aatcgcgcca tgacgatgat
     1921 cgtcacgatt tgcatcacgc cctgaagcgc cacggcgaag aaatagcgat tggtcagcgc
     1981 gccgatcctc gcattgtccg gattgtcttt ctgcagctcc aaacagacgc ggaaattcgt
     2041 gggcagcaaa taaccaattc cctgaatcgt catgaggacg accagc
//
LOCUS       DATNOI010000081        12044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2463964,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000081 DATNOI010000000
VERSION     DATNOI010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12044
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2463964"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1049)
                     /locus_tag="VN968_02800"
     CDS             complement(189..1049)
                     /locus_tag="VN968_02800"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HXL28212.1"
                     /translation="MNSPNGDKASADFCARLVRTHDFVRYASTLFVPAEQRRALLAIY
                     AFNVEISRVREQVSQPLPGEVRLQWWTDMLAGAGHGGIEGNPVAAELKLAMRQWRLPV
                     ERLSRLIDEHQFDLYNDPMPTMAALEGYINDTASALFSLGAGIAGPQSDAVEHLARHA
                     GLAQGIARVLAALPLDASRRQLFVPLQLLQSHGSSMEEVFAGKWTPNLRAALDQLIGE
                     ARGHLKTAFALLETAPSEVRPVFLPLVLVGRDLERISRADNDPFAPLVRSRFRTLWAL
                     WRASRSREFS"
     gene            complement(1046..1429)
                     /locus_tag="VN968_02805"
     CDS             complement(1046..1429)
                     /locus_tag="VN968_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HXL28213.1"
                     /translation="MASRSDAPHLPRSAPIDAYGNGGFAFAEMSHRGSLLCLPDAIWA
                     WPVAKPEEIDRGALERVFAAADGIDTLIVGTGTGVWLPPSELREALRAVQVVLDTMQT
                     GPAVRTYNIMIGERRRVAAALIAVP"
     gene            complement(1432..2436)
                     /gene="secF"
                     /locus_tag="VN968_02810"
     CDS             complement(1432..2436)
                     /gene="secF"
                     /locus_tag="VN968_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HXL28214.1"
                     /translation="MTHFVLIALGVLIAVLTVVSALGLFPSLRIVPDDTHFDFTRFRR
                     ISFPISAALSIVAITLFFTHGLNFGIDFKGGTLMEVRAKSGTADIASMRATLSTLGLG
                     EVQLQQFGGPDQVLIRVAEQPGGDAAQQEAVQKIRSVFGDTVEYRRVEVVGPRVSGEL
                     LAYGMLGLMLAIFAILIYLWFRFEWQFALGAMIANVHDIVLTIGFMSISQVDFDLTSI
                     AALLTILGYSLNDTVVIYDRIREMLRRYKKMPMPQLLNESINSTLSRSIVTHVTVTLA
                     LLALLLFGGHAIHSFTAVMMFGVVLVGTYTSIFIAAPILIYLGVGTHRAEVPDKPFKK
                     "
     gene            complement(2448..4049)
                     /gene="secD"
                     /locus_tag="VN968_02815"
     CDS             complement(2448..4049)
                     /gene="secD"
                     /locus_tag="VN968_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HXL28215.1"
                     /translation="MLYFTRWKALAIILTALVVCLCAVPNFFPEATVKGWPAWAQRRL
                     VLGLDLQGGSYLLLEVDSNYVKKEKLDQIRDDIRRSLRDAKIGYTGLAVRNDAAEVRI
                     SRDTDVGNALAKLRELSQPLGGLLGSSGQRSLEVSDAGGGLIRITVPQAAITERIRQT
                     IEQSIQIVERRVNQLGTVEPVIQRQGTDRILVQVPGLQDPSRLKELLGKTAKMEFRMV
                     DPTVPPDQAQQGRVPPDSEVLMSSTSPKTPYVIKKQVLVSGGDLTDAQPGFDQRSGEP
                     IVSFRFNTSGSRKFAQATSENVGQPFAIVLDNEVISAPVIREPITGGSGQISGSFTVQ
                     QANDLAILLRAGALPAPLTVIEERTVGPGLGQDSIEKGELAAYVGSIMVIVFMLVTYR
                     LFGVFANVAVAINVAMIFGVLSLLNATLTLPGIAGIVLTVGIAVDSNVLIYERIREEL
                     RGGRNAISAIDAGFKRALSTILDSNITTFIAAAVLFYIGTGPVRGFAVTLGIGIITTV
                     FTAFTLTRLIVAWWVRWKRPQTVPI"
     gene            complement(4095..4460)
                     /gene="yajC"
                     /locus_tag="VN968_02820"
     CDS             complement(4095..4460)
                     /gene="yajC"
                     /locus_tag="VN968_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXL28216.1"
                     /translation="MLITPAFAQAAGGGDTNSMLMSLLPFALIFVIMYFLILRPQQKK
                     VKDHAELVKNIRRGDTVVTSGGLVGKVTKVVDDDQIEFEISDGVRVRQMRQMISGVRA
                     KGEPAKEKSEPARDETSAS"
     gene            4653..5612
                     /locus_tag="VN968_02825"
     CDS             4653..5612
                     /locus_tag="VN968_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL28217.1"
                     /translation="MSKKLRKTRARLSRKSAITATKRPAAASSEHDMTERIAHALEAI
                     ASHLAAASPTPGAGDSFGTADAFVWHPNGRLAPVPRVSRVDLGLLKGIDRMRDILIEN
                     TARFAKGLPANNALLWGARGMGKSSLVKATHASINVDREPSERLKLIEIHREDIESLP
                     ALMALLRTSDFHFIVFCDDLSFDGNDASYKSLKAVLEGGIEGRPDNVILYATSNRRHL
                     LPRDMMENERSTAINPGEAVEEKVSLSDRFGLWLGFHRCSQDEYLAMVKGYCGHFGIE
                     LAEDELEREALEWSTTRGSRSGRVAWQFTQELAGRLGVRLSGK"
     gene            5640..6632
                     /locus_tag="VN968_02830"
     CDS             5640..6632
                     /locus_tag="VN968_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL28218.1"
                     /translation="MKRRAILAGIAALLVPRSLTAQTTSRDGPRRLGVLMGNLADDPV
                     GQTYTASLKQGLRALNWHEGGNLRIDWRWAGGDPALFDRYATELVALGSDVLLAQASP
                     SVVALRRKTSTIPIVFTMVTDPIGQGFVENLARPGGNITGFSDFNSLMAGKWLEMLTQ
                     VAPPVARVAVLYNPATAPYAGLMMRAVEDAAPSFAITAQSAPCRDAAEIRSMMAELAR
                     QGRGGLLVLTDIFTIVHRDTILASAAQHRLPTVYFARSFTTAGGLMSYGIDYAELFLR
                     SAAYVDRILKGASPRDLPVQQPTKFELVINLKTAKEGGINLSTTLLATADEVIE"
     gene            complement(6658..7995)
                     /locus_tag="VN968_02835"
     CDS             complement(6658..7995)
                     /locus_tag="VN968_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXL28219.1"
                     /translation="MFRVVELLYSRRVPHVAVLALMSIGFAGCSGDMSTRLSENPFSN
                     PFASQPEATASVPSAPVERRELPQYARPQSPGQSSAPEIAAPQSYPTASAGSSGGGRG
                     VTSYAAPGQPLETTGSAAPRSVAANRGSGQGGTKIIVGTSDTLDTLSRRYNVSPAAIL
                     QANGYKGPRVLSPGQQLIIPRPTVAAAAPVPAAPASKAVAATTAAPSIHVVNRGDTLL
                     SIAHRNRVSVAELARANGLDPQAKLKLGTKLTVPGAKTAAVAPAAQPAAAAQPATALA
                     PPATKIAAATGEPQQKVRLAQPTTTPAELSPETPIKASEATGALPTFRWPVRGKVITS
                     YGAKTNGKANDGINLAVPEGTPVKAAEEGVVAYSGNELKGYGNLVLVRHSNGYVTAYA
                     HASELLVKRGDTIKRGQIIAKSGQSGEVGSPQLHFEIRKGSSPVDPLQFLNGA"
     gene            complement(8105..8755)
                     /locus_tag="VN968_02840"
     CDS             complement(8105..8755)
                     /locus_tag="VN968_02840"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXL28220.1"
                     /translation="MPATRKPPEKMMFQLTLRRRGISDQAVLRAMEEIPREMFVDAAD
                     RADAYRDSALGIACGQTISQPFVVAYMTEQLQLQKHHRVLEVGTGSGYQAAILSRLCG
                     HVLTVERYRTLAESARARLEKLGCDNVEVMFGDGFEIPASAGKFDRIIVTAAMEQIPD
                     SLTQRLEPAGVLIAPLGPHHGTQTLVRVTRTDAGFERKELVDVRFVPALPGIAREL"
     gene            9051..9428
                     /locus_tag="VN968_02845"
     CDS             9051..9428
                     /locus_tag="VN968_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL28221.1"
                     /translation="MIATRPPGGSVFLVEDEVMIRMMVADMLEELGYSVAAEAGEINE
                     AIRLAQSAQFDLAILDVNVNGKVISPVADLIDSRSIPFIFATGYGSSGLPEEYRDRPA
                     LQKPFQVETLGRMIEVALKRATV"
     gene            9507..9863
                     /locus_tag="VN968_02850"
     CDS             9507..9863
                     /locus_tag="VN968_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXL28222.1"
                     /translation="MGVYVYMLRCADGSYYVGSATGDDLSTRIDQHNAGSHKGYTFSR
                     RPVVLVSSEYFERITDGIAVERQLKGWSRAKKEALMRSDWTKVSELSRRRGGLAKQAL
                     PKNSSFEARKERALSG"
     gene            complement(9883..10650)
                     /gene="surE"
                     /locus_tag="VN968_02855"
     CDS             complement(9883..10650)
                     /gene="surE"
                     /locus_tag="VN968_02855"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HXL28223.1"
                     /translation="MRILCTNDDGIHAPGLKIVEEIARALSDDVWIVAPELDQSGVSH
                     SLSLNDPLRLREVGPRHFAVRGTPTDCVIMGARHILGETMPDLVLSGVNKGRNVAEDV
                     VYSGTIAGALEGTILGLPSFALSQEFSMETRNAPQWETALKFGPQILRKVIDAGVPKN
                     TVINVNFPACAPDDVAGIRVTRQGKRNLGFLKVDERRDGRGNPYFWIGFERLAQIDVP
                     AEDTDLAALAARYVSVTPLRLDRTDDEFSQALSATLK"
     gene            complement(10844..11530)
                     /locus_tag="VN968_02860"
     CDS             complement(10844..11530)
                     /locus_tag="VN968_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXL28224.1"
                     /translation="MSHTSQTRVLQKGFEPRAFLARAGGGKTVERYAKGQKVFSQGEI
                     AGTVFFIQEGKVKLTVLSELGKEAVVGIFAEGQFFGEGCLGGAELRAATSHAMEDCLI
                     TSITRKAMLAALESEPEFSAFFIAYLLSRNSRIEDDLIDQLFNSSERRLARLLLRLAN
                     FGQQGGAIAITLSQETLAEMIGTTRSRVSFFMNKFRKKGFISYNGKIEVYPSLLDAVL
                     GDKPQINDDE"
     gene            complement(11741..>12044)
                     /locus_tag="VN968_02865"
     CDS             complement(11741..>12044)
                     /locus_tag="VN968_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28225.1"
                     /translation="HIQLSDSFGSSELGNALKSTRSIMPEFQPVASGVVSAIERPKCP
                     KCGQNRMLLSKLEAAGSDYRTFECQRCGCIHKVLVSNDPLTSDMVGWLASELRPPT"
BASE COUNT         2342 a         3770 c         3699 g         2233 t
ORIGIN      
        1 gaccggttta attttgaagt ggcggacttg gtgccaccgt ccccaacgga cgtcatcacc
       61 cgcgaaggcg ggtgatccag tatttcagag cattagtgat agactgagaa gccgcggcgt
      121 actggatact ccgctttcgc ggagtatgac aatcacggtt agcgcatgta gcgtttcgtc
      181 atcctcgttt aggaaaactc ccgcgaccgc gacgcgcgcc acaatgccca caaggtccga
      241 aaccgcgatc tgaccagcgg cgcgaacgga tcgttgtccg cgcgcgatat ccgttcgaga
      301 tcgcgcccaa ccagtaccag cggcaggaac accggcctca cttccgacgg cgccgtttcc
      361 agaagcgcga aggctgtctt caaatgtccc cgcgcttcgc cgatcaactg atccagcgcc
      421 gcccgcaaat tcggcgtcca cttgcccgcg aacacttctt ccatcgagct gccgtggctc
      481 tggagcaatt gcagcggcac gaacaactgc cgtcgcgagg cgtccagcgg caatgctgcc
      541 agcacccgcg cgatgccttg cgccagtccg gcatgacgcg ccaggtgctc gaccgcatcg
      601 gactgcggcc cggcaatgcc cgcaccgagc gaaaacaacg ccgacgcggt gtcgttgata
      661 tagccttcca gcgccgccat ggtcggcatc ggatcgttgt agagatcgaa ctggtgctcg
      721 tcgatcagcc gcgacagccg ctcgaccggc aagcgccatt gccgcatcgc cagcttgagt
      781 tcagccgcaa ccggattgcc ctcgatcccg ccgtggcccg cgccggccag catgtcggtc
      841 caccattgca gccgcacctc gccgggcagc ggctggctca cctgctcgcg aacacgggaa
      901 atctcgacat tgaaggcgta gatcgccagc agcgcccggc gctgctcggc gggtacgaac
      961 agcgtggagg cgtaacggac aaaatcgtgg gtgcgcacca acctggcgca gaaatcggcg
     1021 gacgctttgt ctccgttcgg gctgttcatg gtaccgcgat caacgccgcc gcgacacgcc
     1081 gccgctcgcc gatcatgatg ttgtaggtgc gaacggcagg acccgtctgc atcgtatcga
     1141 gcaccacctg gaccgcgcgc aacgcctcgc gaagctcgga tggcggcaac cagacacccg
     1201 tgccggtacc gacgatcagg gtatcgatgc catcggccgc ggcgaatacc cgctccagtg
     1261 cccccctgtc gatttcctcc ggtttcgcaa ctggccaggc ccagatcgca tccggcaggc
     1321 acagcaggga gccgcgatgc gacatctcgg caaaagcaaa accgccattg ccgtaagcat
     1381 cgatcggcgc cgacctcgga agatgcggag cgtccgagcg gctcgccatc gtcatttctt
     1441 gaagggcttg tccggcacct ctgcccggtg ggtgccgacg ccgagataga tcaggatcgg
     1501 cgcagcgata aagatcgagg tgtaagtgcc gaccagcacc acgccgaaca tcatcacggc
     1561 ggtgaagctg tggatggcat ggccgccgaa tagcaatagc gccaacagcg ccagcgtgac
     1621 cgtgacgtgg gtgacgatcg agcgcgatag cgtcgagttg atcgattcgt tgagcaattg
     1681 cggcatcggc attttcttgt agcgccgcag catttcgcgg atccggtcat agatcacgac
     1741 ggtgtcgttc aacgaataac cgagaatcgt caacagcgcc gcgatgctgg tgagatcgaa
     1801 atcgacctgt gagatcgaca tgaagccgat ggtcagcacg atgtcgtgga cgttggcgat
     1861 catggcgccg agcgcgaact gccattcgaa gcggaaccag agatagatca ggatcgcaaa
     1921 gatcgcgagc atcaggccga gcatgccgta ggccagcaat tcgccggaaa cgcgcggtcc
     1981 caccacctcg acgcgccggt actcgacggt atcgccgaac acgcttcgga ttttctgcac
     2041 cgcttcctgc tgcgcggcgt cgccgccggg ttgctcggca accctgatca gcacctggtc
     2101 cggcccaccg aactgctgca actgaacttc gccaagcccg agggtactga gagtggcccg
     2161 catcgacgca atatcggcgg taccggattt ggcgcgcact tccatcagcg tgccgccctt
     2221 gaaatcgatg ccgaaattaa gcccgtgggt aaagaacagc gtgattgcga caatcgagag
     2281 cgctgccgag atcggaaaac tgatgcgccg gaagcgggta aaatcgaagt gcgtatcgtc
     2341 cgggacgatc cgcagcgagg gaaacaggcc gagcgcgctg accacggtca gcacggcaat
     2401 caaaacgccg agcgcgatga gaacgaagtg ggtcaaggct gcattcccta gatcggcacc
     2461 gtctgcggcc gcttccagcg cacccaccac gccacgatca ggcgggtcag ggtaaacgcg
     2521 gtgaagaccg tggtgatgat gccgatgccg agcgtcacgg caaaaccgcg taccggcccg
     2581 gtcccgatat agaacagcac cgcggcggcg atgaaggtgg tgatgttgga atccaggatc
     2641 gtcgacagtg cccgcttgaa accggcgtcg atcgcggaaa tcgcgttgcg cccgcctcgc
     2701 aattcctccc ggatgcgctc atagatcagg acgttggagt ccaccgcgat gccgacggta
     2761 agcacgatgc ctgcgatgcc gggcagcgtc agcgtggcat tcaacagcga cagcaccccg
     2821 aagatcatcg caacgttgat ggcgaccgcg acattggcga acacgccgaa cagccggtag
     2881 gtcaccaaca tgaacacgat gaccatgata gagccgacgt aagcggcaag ttcgcccttt
     2941 tcgatcgaat cctgcccgag ccccggaccg acggtgcgct cctcgatgac ggtcaacggt
     3001 gccggcagcg cgcccgcgcg cagcaggatc gccagatcgt tggcctgctg caccgtgaag
     3061 ctgccggaga tttggccgga gccgcctgtg atcggctcgc ggatcaccgg cgcggaaatc
     3121 acctcgttgt cgagcacgat cgcgaagggt tgtccgacat tctccgacgt cgcctgcgcg
     3181 aatttgcgtg agccggaggt attgaagcgg aagctgacga tcggctcgcc cgagcgctga
     3241 tcgaagcccg gctgcgcgtc ggtcaaatcg ccgccggaaa ccagaacctg tttcttgatg
     3301 acatagggtg ttttcggcga cgtcgaactc atcagcactt cggaatccgg cggcaccctg
     3361 ccctgttggg cctgatccgg cggcacggtc ggatcgacca tgcggaattc catcttggcg
     3421 gtcttgccca ggagctcctt caggcggctc ggatcctgca agcccggcac ctgcaccaga
     3481 atgcgatcgg tgccctgccg ctgaattacc ggctcaacag tgccaagctg attgacgcgg
     3541 cgctccacga tctgaatcga ttgttcgatg gtttgccgta tccgttcagt gatcgcggcc
     3601 tgcggaacgg tgatccggat caacccaccg ccggcgtcac tgacctcaag gctgcgctgg
     3661 ccgctggagc cgagcaagcc gccaagcggt tgcgacagct cgcgcaactt tgccagcgca
     3721 ttgcccacat cggtgtccct gctgatgcgc acttccgcgg cgtcattgcg cacggcaagg
     3781 ccggtataac cgatcttggc gtcccgcagg ctgcggcgga tgtcgtcgcg tatctggtcg
     3841 agcttctcct tcttgacgta gttggagtcg acctcaagca gcaaatagga gccgccctgc
     3901 agatcgaggc cgagcaccag ccgccgctgc gcccatgccg gccagccctt gacggtcgct
     3961 tcgggaaaaa aattcggaac ggcgcaaagg cacactacca gcgctgtaag aatgatcgcc
     4021 agcgccttcc accgcgtgaa atacaacatc gaatttgact cgtcagaccc aggagaatgc
     4081 agcgcgtaaa aaagctagct cgcggacgtc tcgtccctgg cgggctcact cttttccttc
     4141 gccggctcac ccttggcgcg aacgccggaa atcatctgcc gcatttgccg cacgcggacc
     4201 ccgtccgaaa tttcgaactc gatctggtcg tcgtcgacta ccttggtcac tttgccgacc
     4261 agcccgcccg aggtgacaac ggtgtcgccg cggcggatat tcttcacgag ttcggcgtgg
     4321 tccttcacct tcttctgctg cggccgcaga atcaggaaat acatgatcac gaagatcagc
     4381 gcgaacggca gcagcgacat caacatgctg ttggtatcgc cgccacccgc agcctgggca
     4441 aacgcaggag taatcagcat tcggacaatc ctcgtgagac gggagagaac cggcaacgca
     4501 ttcgcatcac gccggttcgg gcaaattcgc gcggactata gcggtcgcgg tcccaattgc
     4561 aacgctgcca gacccctcat tttgcccttc atttgagtgg cttgcggccc accgaaaggg
     4621 ccgatatggc tgcatgatca ggaaccccaa agatgtcgaa aaagcttcga aaaacaagag
     4681 cccgcctctc gcgaaaatcg gcgataaccg cgacaaaacg tccggcagca gcttcttccg
     4741 aacatgacat gacggaaagg atcgcccacg cgctcgaggc gatcgcctcg caccttgccg
     4801 ccgcatcgcc gacgccaggc gctggcgact cattcggcac cgccgacgcc ttcgtctggc
     4861 acccgaacgg acggcttgcg ccggtaccgc gcgtcagccg cgtcgatctc ggtctgctca
     4921 agggtatcga tcggatgcgc gatatcctga tcgagaatac cgcgcgcttc gccaaaggac
     4981 ttcccgccaa caacgcgctg ttatggggcg cgcgcggcat gggcaaatcg tcgctggtca
     5041 aggcgacgca tgccagcatc aatgtggacc gcgagccttc agagcgtttg aaactgatcg
     5101 agatccatcg cgaggatatc gaaagcctgc cggcgctgat ggcgctgctg cgcacctcgg
     5161 attttcattt catcgtgttc tgtgacgacc tgtcgttcga cggcaacgat gcctcctaca
     5221 agtcgctgaa ggcggtgctg gaagggggca tcgagggccg gcccgataat gtcatcctgt
     5281 acgccacctc gaaccgcagg catctcttgc cgcgtgacat gatggagaac gaacgctcca
     5341 ccgcgatcaa tccgggcgag gcggtcgagg aaaaggtctc gctctcggac cggttcggat
     5401 tgtggctcgg ctttcaccgt tgcagccagg acgaatacct tgcgatggtg aagggctatt
     5461 gcggccattt cggcatcgag cttgccgaag acgagcttga gcgcgaggcg ctggaatggt
     5521 cgaccacgcg cggctcgcgt tcgggccgcg tcgcctggca gttcacgcag gaattggcgg
     5581 gaaggcttgg ggttaggctg agcgggaagt aagcaccgcg ccaggggagg ctgtctgtca
     5641 tgaaacgccg cgcgatcctc gcgggaattg ccgctctgct tgtgccgcgc tcgttgactg
     5701 cgcagacgac gtcgcgcgac ggaccgcgcc gcctcggcgt gctgatgggc aatctcgccg
     5761 acgacccggt gggccaaacc tacacggctt cactgaagca gggccttcgc gcgcttaact
     5821 ggcacgaggg aggaaatttg cgaattgact ggcgctgggc cggcggcgat cccgcacttt
     5881 tcgatcgtta tgcgacggaa ctggtggcgc tcggctccga tgtgcttctt gcgcaagcca
     5941 gcccatccgt cgtggcgctg cggcggaaaa caagcacgat cccgatcgtg ttcacgatgg
     6001 tcaccgatcc gatcggccag ggctttgtcg agaacctggc gcgtccgggc ggcaacatca
     6061 ccggcttcag cgatttcaac tcgctaatgg ccggcaaatg gctggagatg ctgacccagg
     6121 tcgcgccgcc cgttgcacgc gtggctgttc tctacaatcc cgctaccgcg ccctatgccg
     6181 gtctgatgat gcgcgccgtc gaggacgcag caccctcctt cgccataacg gcacaaagcg
     6241 cgccatgccg tgacgccgcc gagatccggt caatgatggc ggagcttgcc cgccaggggc
     6301 gcggtggatt gctggtcttg accgatattt tcaccatcgt gcatcgcgac accatcctcg
     6361 cttccgccgc gcagcatcgc cttcctaccg tgtatttcgc gcgctcgttc accacggccg
     6421 ggggcctcat gtcctacggc atcgactacg ccgagctgtt ccttcgctcg gccgcctatg
     6481 tcgatcgtat tctgaagggc gccagcccgc gcgacttgcc ggtccagcag ccaaccaagt
     6541 tcgagctggt gatcaacctc aagaccgcca aagagggcgg cattaacctc tcgacgacgc
     6601 tgcttgctac cgcagacgag gtgatcgagt gagatggtcg tcgcctccgc gccgggttca
     6661 cgccccattg aggaattgaa gcgggtcgac cggcgaagag cctttgcgga tctcgaaatg
     6721 gagctgcgga gatccgacct cgcccgactg gccggacttg gcaatgatct ggccgcgctt
     6781 gatggtgtcg ccgcgcttca ccagcagttc actggcatgg gcataggcgg tgacgtagcc
     6841 gttcgagtgc cgaaccagaa ccaggttgcc gtaacccttg agctcattgc cggagtaggc
     6901 gacgacaccc tcctcggccg ccttcaccgg cgtgccttcc ggcaccgcca gattgatgcc
     6961 gtcattggcc ttaccgttgg tcttggcgcc gtagctggtg atcaccttgc cgcgcaccgg
     7021 ccagcggaat gtcggcagcg cgccggttgc ctcgctggcc ttgatcggcg tttcgggcga
     7081 aagctccgcg ggcgtcgtcg tcggctgcgc cagccggacc ttttgctggg gttcgcccgt
     7141 tgctgccgcg attttggtcg cgggcggtgc caatgccgtg gccggctgtg cggcagcggc
     7201 cggctgcgcg gccggcgcaa cggctgccgt cttggcgccg ggcacggtga gcttcgtgcc
     7261 gagcttgagc ttcgcctggg gatcgaggcc gttggccctc gcaagttcag cgaccgacac
     7321 gcgattgcgg tgggcgatgc tgagcaaggt atcgccgcga ttgacgacat gaatcgacgg
     7381 cgctgcggta gtggcggcaa cggccttgct tgccggcgcg gccggcaccg gggcggcagc
     7441 cgccacggtc ggccgcggaa tgatcagctg ctggcccggc gacagaacgc gcggcccctt
     7501 gtaaccgttg gcctgcagga tcgcggcggg cgaaacgtta tagcggcgcg acagggtatc
     7561 gagtgtatcg ctggtgccga cgatgatctt ggtgccgccc tgccccgatc cgcggttggc
     7621 cgcgaccgag cgcggcgcgg cactgccggt ggtctcaagc ggttggccgg gcgcagcata
     7681 ggaagttacc ccgcgccctc ctcctgagga gccggcgctg gcggtcggat aggattgcgg
     7741 cgccgcgatc tcaggggccg aggattggcc cggcgattgc gggcgggcat attgcggtag
     7801 ttcgcggcgc tcgaccgggg cgctcgggac cgaggccgtg gcttccggtt gcgaggcaaa
     7861 gggattggaa aagggatttt ccgagaggcg cgtcgacatg tcgccgctgc agccggcaaa
     7921 accaatcgac atcagcgcca gcaccgccac gtgcggcaca cggcgtgagt aaagcaactc
     7981 gacgacacgg aacatggtta ctcactcgta cgcaactaac tatcgttttg agtaaacacc
     8041 tccctagtaa agaagctctt aagcctgcga ataagacgtt ggcggcacag ctcaaaccga
     8101 gcctttacag ctcgcgggcg atgcccggca acgccggcac gaaacgcacg tccaccagct
     8161 ccttgcgctc gaaaccggca tcggtcctgg tcacgcggac caaggtctgg gtgccatgat
     8221 gcggcccgag cggggcgatc agtacgcccg ccggttcaag ccgctgcgtc aagctatccg
     8281 gaatttgctc catcgccgcg gtgacgataa tgcggtcgaa tttgccggcg ctggccggaa
     8341 tctcgaagcc gtccccgaac atcacctcga cattgtcgca gccgagcttc tcaagccgcg
     8401 cccgggcgct ttcggcgagc gtccggtaac gctcgacggt gagaacgtgg ccgcagagcc
     8461 gcgacaggat ggcggcctgg tatccggagc cggtgccgac ctccagcaca cggtgatgct
     8521 tctgcagctg caactgctcg gtcatgtaag ccaccacaaa tggctggctg atggtttgcc
     8581 cgcacgcgat gccgagcgcg ctgtcacgat aggcatcggc gcgatcggcc gcgtccacga
     8641 acatttcgcg cggaatctcc tccatagcgc gaagcaccgc ctgatcgctg atcccccggc
     8701 gtcgcaaggt gagctgaaac atcatctttt cgggcggttt ccgagtagca ggcatgtcgg
     8761 tctcgcaagc tgggcgcgaa tttctgaatc gggacagtac cgcgccgcca agcaggttga
     8821 tgcaatatgg ccccgggggc ttaaggaagg aatttcccgc aattccggtt ccagagaggg
     8881 aactggatcc catatctggc gttggaaggc cgggcaatca atcttggtca aggccccgca
     8941 aaacaacgct catgacgacg ggcaacaatg cgacgtggtt gcgcacgttt acattgcgcc
     9001 caagggcgtt ttttgcgaac cttgatctcg ggatgggcaa ggactcagtc atgattgcga
     9061 cgcggccgcc gggcggttct gtgttcctcg ttgaagacga agtcatgatc cggatgatgg
     9121 tcgccgacat gctggaggag ctgggctaca gcgtcgcggc cgaagccggc gagatcaacg
     9181 aggcgatcag gctggcccaa tccgcccagt tcgacctcgc cattctcgat gtcaacgtca
     9241 atggcaaggt gatatcgccg gttgccgacc tgatcgattc gcgcagcatc ccctttatct
     9301 ttgccaccgg ctatggctcg tccggactcc cggaagagta ccgcgaccgc ccggcgctgc
     9361 aaaagccttt ccaggtcgaa accttgggcc gcatgatcga agtcgcgctg aagcgggcga
     9421 cggtttagat tcgagagcaa ttgacagctg accaatcgac gtcatcctga ggagcgagcg
     9481 cttgcgagcc tcgaaggacg acgtcgatag gtgtttatgt ctacatgttg cgctgcgcag
     9541 acggctccta ttacgttggg agcgcgactg gagatgacct ctcaacccgc atagatcagc
     9601 ataatgcggg atcccacaag ggttatactt tttcccgacg acccgtcgtt cttgtctcgt
     9661 ctgagtattt cgagcgtata accgatggta tcgcagtcga acgccagctc aagggttgga
     9721 gccgtgccaa aaaagaggcg ctgatgcgct ccgattggac gaaggtaagt gaattgtccc
     9781 ggcgtcgtgg tggtttggca aagcaggctt tgcctaagaa ttcatccttc gaggcgcgca
     9841 aagagcgcgc actctcagga tgacgtctgt gctcgtggcc cgctatttca acgtcgcgct
     9901 cagcgcttgt gagaactcat cgtcggtgcg gtcaaggcgc agcggcgtca ccgagacgta
     9961 acgcgccgcc agcgccgcaa gatcggtgtc ctccgccggc acgtcgatct gcgccagccg
    10021 ttcaaatccg atccagaaat atgggttgcc gcggccgtcg cggcgctcgt cgaccttgag
    10081 aaagccgaga ttgcgcttgc cctgccgcgt cacgcgtatg cctgcgacgt catcgggcgc
    10141 acaggccggg aagttgacat tgatcaccgt gttcttgggc acgccggcat cgatcacctt
    10201 gcgcaaaatc tgcggcccga atttcagtgc ggtctcccac tgcggcgcgt tgcgggtctc
    10261 catgctgaac tcctgcgaca gcgcaaacga cggcaagccc agaatggtgc cctccaaagc
    10321 gccggcgatg gtgccggaat acacgacgtc ttcggcgacg ttacggccct tgttgacgcc
    10381 ggacagcacc aggtccggca tggtttcgcc gaggatgtgg cgcgcgccca tgatgacgca
    10441 atcggtcggc gtgccccgca ccgcgaaatg acgcggcccg acttcgcgca accgcaaggg
    10501 atcgttcagc gacaaggaat gcgagacgcc ggactgatcg agttccggcg ccactatcca
    10561 cacatcgtcg gacaatgcgc gggcgatctc ctcgacaatc ttcaagccgg gagcatggat
    10621 gccgtcgtca ttggtgcaga gaattcgcat ccgttcaacg ccttgttcgg tcggttccac
    10681 cagcggtcaa ggcgccgtct tatccttctc acgccgccga ggcaaacccg gcaatatcga
    10741 gcaagatggc tgctgcgcca ggctgtgcgc gcggaaggtg tgatcgcgcg acgcgcgagc
    10801 gctctcgtgt ttgcatgatt ggggttcgag ctggccgccg ccgctactcg tcgtcgttta
    10861 tttgcggctt gtctccaagc accgcatcca gcagcgaggg gtagacctct atcttgccgt
    10921 tgtagctgat gaatcccttt ttcctgaatt tgttcatgaa aaaactcacc cgtgatcgcg
    10981 tggtgccgat catttcggcc agcgtttcct gactgagggt gatcgcgatc gcgccgcctt
    11041 gctggccgaa attggcgaga cgcaaaagca gccgcgccag gcgccgttcg ctggaattga
    11101 aaagctggtc gatcaaatcg tcttcgatcc ggctgttgcg ggacaggaga taggctatga
    11161 aaaacgcgga aaactccggc tcgctttcca gggccgcaag catcgctttc cttgtaatcg
    11221 acgtgatcag gcagtcctcc atggcgtggc tggtggcagc gcgtaattcc gccccgccaa
    11281 gacatccttc gccgaaaaac tgcccttccg cgaaaatccc aaccaccgct tccttgccga
    11341 gttcggataa aaccgtgagc ttgaccttgc cctcctgaat gaaaaacacg gtgccggcga
    11401 tctcgccctg gctgaaaacc ttctggcctt tggcgtatcg ctcgacggtt tttccgcccc
    11461 cggcgcgggc aagaaaagcc cggggttcaa accccttctg caaaaccctg gtttgcgatg
    11521 tgtgggacat ggtgctgctc cgtcatcagc ccgggagcac gacacgctca ttcaccggca
    11581 aaaccggcga ggcctggtga tggctcagta taacccagct atgtccggta ggcgacttaa
    11641 attggaattc ggcaccgcgc cgctttggcc cagttcccgc aagggaacag acgtatccgc
    11701 cgctccactt gcatcgcagg cttgactgaa aatgaagccg ttatgtcggc ggccttagct
    11761 cgctggcgag ccagccgacc atgtcggagg tcaagggatc gttggatacc aacaccttgt
    11821 gaatacaacc gcacctctgg cactcgaatg tccggtagtc agagccggcc gcctcgagct
    11881 tcgacagcag catgcggttt tggccgcatt tcgggcattt cggccgctcg atggcggaaa
    11941 caaccccaga cgctaccggt tgaaattcgg gcatgatgct tcgcgttgat ttgagagcgt
    12001 tgcctaactc actggaacca aaactatcag agagctggat gtga
//
LOCUS       DATNOI010000082         2867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_518115,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000082 DATNOI010000000
VERSION     DATNOI010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518115"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VN968_02870"
     CDS             <1..1083
                     /locus_tag="VN968_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXL28226.1"
                     /translation="AVNALGHAHPHLVAALQEQATKLWHMSNLFKSPDGERLAARLCE
                     QSFADLVFFANSGAEAMECAIKITRKYHAAKGHPERYRIITFDGAFHGRTLATLAATG
                     SAKYLEGFGPPMDGFDQVTLGDLDAVKKAIGPHTAGILIEPLQGEGGVRAAPHAFFKA
                     LRQLCDDNGLLLIFDEVQTGMGRTGELFAYKRLGVTPDVMPLAKALGGGFPIGACLAT
                     ADAAAGMTPGSHGSTFGGNPLAVAAANAVLDVMLKPGFFEHAQKMSLLLKQKLASVVD
                     RHPKVLAEVRGEGLLIGLKAVVPSGDLVTALRDQKLLTVGAGENVVRFLPPLIVTEAE
                     IEESVARLERACTALSGDQQKRAAGQ"
     gene            1080..2018
                     /gene="argF"
                     /locus_tag="VN968_02875"
     CDS             1080..2018
                     /gene="argF"
                     /locus_tag="VN968_02875"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HXL28227.1"
                     /translation="MSKALRHFLDLSELPTRELRNMLAASVAMKAKLKAQKKQDAELV
                     KPLEGKTLAMIFEKPSTRTRVSFDVGMRQLGGEAIMLTGAEMQLGRGETIADTARVLS
                     RYVDAIMIRILNHDALLELAAHATVPVINGLTRRSHPCQVMADLMTFEEHRGPIEGRT
                     IAWTGDDNNVLASWAHAAERFRFNLHVATPPQLAPNKSMRDWIKATQAPIVLGNDPEA
                     AVRDADCVVTDTWFSMGDKDGEHRHKLLKPYEVNAKLMSLAKPDALFMHCLPAHRGDE
                     VTDEVIDGPQSVVFDEAENRLHAQKGILAWCFDAVA"
     gene            2301..>2867
                     /locus_tag="VN968_02880"
     CDS             2301..>2867
                     /locus_tag="VN968_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HXL28228.1"
                     /translation="MTSQFPDMKISLEASIRAPSAVPIDDAVLPFEVSSLDLRGRLTR
                     LGPALDDVLAKHAYPPAVGKLLGEAMVLTTLLGSSLKFDGRFILQTQTDGPVSFLIVD
                     FQAPDRLRAYARFDATRLEDGLDSGALLGHGHLAMTIDQGPDMSRYQGLVALEGGSLE
                     DAAHEYFLRSEQIPTRVRLAVGEECRGGE"
BASE COUNT          530 a          905 c          931 g          501 t
ORIGIN      
        1 gcggtgaatg cgctgggcca tgcgcatccg catctggtcg cagccttgca ggaacaggcg
       61 acaaaactgt ggcacatgtc gaacctgttc aagagcccgg atggcgagcg gctcgcggca
      121 cggctgtgcg aacaaagctt cgccgacctt gtgtttttcg cgaactccgg cgccgaggcg
      181 atggaatgcg cgatcaagat cacgcgcaaa tatcacgccg ccaaaggtca cccggagcgc
      241 tatcgcatca tcaccttcga cggcgcgttt cacgggcgga cgctggcgac gctggctgcg
      301 accgggtctg ccaaatatct cgaaggcttc gggccgccga tggatggctt cgatcaggta
      361 acgctcggcg acctcgacgc cgtcaagaag gcgatcggcc cgcacaccgc gggcatcctg
      421 atcgaaccgc tgcagggcga aggcggcgtg cgtgccgcgc cgcatgcgtt cttcaaggcg
      481 ttgcgtcagc tgtgcgacga caacgggctg ctgctgatat tcgacgaggt gcagaccggc
      541 atgggccgca ccggcgagct gttcgcctac aagcgtctcg gcgtcacgcc ggacgtgatg
      601 ccgctggcga aggcgctcgg cggcggcttt ccgatcggtg catgtctggc gaccgccgac
      661 gcggccgccg ggatgacgcc tggctcgcac ggttcgacct ttggcggcaa cccgctggcg
      721 gtggcagcgg cgaacgcggt gctggacgtg atgctgaagc ccggtttctt cgagcatgcg
      781 cagaagatgt cgctgctgct caaacagaag ctggcctcgg tagtcgatcg ccatccaaag
      841 gtcctcgcag aagtgcgcgg cgagggactg ctgattggtc tgaaggcggt ggtgccgtcg
      901 ggcgatctcg tgacggcatt gcgtgaccaa aagctgctca cggttggcgc cggcgaaaac
      961 gtggtgcggt tcctgccgcc actgatcgtg accgaggcgg agatcgaaga atccgtggct
     1021 cgcctcgagc gcgcctgcac cgcgctttcc ggcgatcagc aaaaacgggc ggcggggcaa
     1081 tgagcaaggc gctgcggcat tttctcgatc tcagcgagct tccgaccagg gagctccgca
     1141 acatgctcgc cgccagcgtc gccatgaagg cgaagctgaa ggcgcaaaaa aaacaggatg
     1201 ccgaactggt caagccgctc gagggcaaga cgctggcgat gattttcgaa aagccctcga
     1261 cccgcacccg ggtgtcgttc gacgtcggca tgcgccagct cggcggtgaa gcgatcatgc
     1321 tgactggcgc ggaaatgcag ctcgggcgcg gcgagaccat cgccgacacc gcgcgggtac
     1381 tgtcgcgtta cgtcgacgcc atcatgatcc gcattctcaa ccacgatgcg ctgctcgaac
     1441 tcgccgcgca cgccaccgtt cccgtcatca acgggctgac acggcggtcg catccctgcc
     1501 aggtgatggc ggatctgatg acgttcgagg agcatcgcgg tccgatcgaa ggccgtacca
     1561 tcgcgtggac cggcgacgac aacaacgtgc tggcgtcatg ggcgcacgcg gcggagcgtt
     1621 tccgtttcaa tctccacgtc gcaactccgc cgcagctcgc gccgaacaaa tcgatgcggg
     1681 actggatcaa ggcgacgcag gcgccgatcg tgctcggcaa tgatccggag gcggccgtgc
     1741 gtgatgccga ttgcgtggtc accgacacct ggttctcgat gggcgacaag gacggcgagc
     1801 accgtcacaa gctgctgaaa ccgtatgagg tcaacgccaa gctgatgtcg ctggcgaaac
     1861 ccgacgcgct gttcatgcat tgcctgcccg cgcatcgcgg cgacgaagtc accgacgagg
     1921 tgatcgacgg cccgcaatcg gtggtgttcg acgaggccga aaatcgcctg cacgcgcaaa
     1981 aaggcattct ggcgtggtgc ttcgacgcgg tggcgtgacc ggttagtcaa acggagaatg
     2041 tgcaacatcg tcgtcccggc gaacgaggtt gtgaatatat ctccgacctc atcctgagga
     2101 gcgcgccctt gcgcgcgtct cgaaggatga ccgcgagtcc gcgctttgcg tccatccttc
     2161 gagacgcttg cttcgcaagc tcctcaggat gaggtcggag atatatttca caacctcgaa
     2221 cccgggacga cggatggaat gatatttcat ctcagtttca ggcctttcca ttcacacccg
     2281 ccgaccccag ataaagcgcc atgacttcac aattccccga catgaaaatc tccctcgaag
     2341 cctccatccg cgcaccatcg gccgttccga tcgacgatgc ggtgctgccg ttcgaggtca
     2401 gttcgctcga tctgcgcggc cggttgaccc ggcttggccc cgcgctcgac gatgtcctgg
     2461 ccaagcacgc ttatccgccg gcggtcggca agctgctcgg cgaggcgatg gtgctgacaa
     2521 ccttgcttgg ctcgtcgctg aaattcgacg gccgcttcat cctgcagacc cagaccgacg
     2581 gcccggtgtc gtttctgatc gtggacttcc aggcgccgga ccgcttgcgc gcctacgcgc
     2641 gcttcgacgc gacgcggctc gaagatgggc tcgattccgg cgcgctgctg gggcacggcc
     2701 atctcgccat gaccatcgat cagggaccgg acatgagccg ctatcagggg ctggtggcgc
     2761 tcgaaggcgg cagcctggaa gacgccgcgc atgaatattt cctgcgctcc gaacagatcc
     2821 cgacccgggt gcggctcgcg gtcggtgagg agtgccgcgg cggcgaa
//
LOCUS       DATNOI010000083        11275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1733335,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000083 DATNOI010000000
VERSION     DATNOI010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11275
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733335"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..255
                     /locus_tag="VN968_02885"
     CDS             37..255
                     /locus_tag="VN968_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HXL28229.1"
                     /translation="MDLKPGDVVILKSGGHSMTVAEVKDGAIACVWMGGEGDLFRETL
                     SSAVLEQVEVEDSDDEEEDEEEDEEEVA"
     gene            complement(316..1083)
                     /locus_tag="VN968_02890"
     CDS             complement(316..1083)
                     /locus_tag="VN968_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HXL28230.1"
                     /translation="MSIISGFADISANQLLLVACVALFASVVGGLAGYGTGALMPLVL
                     VPLIGAEPVVPIIAISSIFTNTSRFAAYLRYADRRRAAIVIAAAALTTALGAYGYTRL
                     TNAGAALAIGAMLISSVPMRCLLKRRDIKISDTGLAAGSVGYGVVVGGTSGPGVILLS
                     LLMAAGLEGAAVIATDAVISVATSIIKISVFGLAGVVTAQVFAFAVLIGAIALPGAFL
                     ARAFVERMPVHVHAAILDVAVIAGGTVMIVEAARSFH"
     gene            complement(1080..2336)
                     /locus_tag="VN968_02895"
     CDS             complement(1080..2336)
                     /locus_tag="VN968_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HXL28231.1"
                     /translation="MTDARVLDDAPWFKSGPAVRVLEMLNGNGEEARVVGGAVRNALL
                     KIPTGDIDIATTAVPDEVIRRAKAAGIKSVPTGIEHGTVTLVVDAQPFEVTTLREDTE
                     TFGRKAKVAFGRDWVRDAERRDFTINGLSVDAGGVVHDHVGGLDDVLAKRVRFIGDPS
                     QRIAEDYLRIPRFFRIHAAYGAGEPDRAGYLACIAARAGLTTLSAERVRMEMLKLMVA
                     EGAPGAVAAMADGGLLLPIFGGVAYIGPFTAMISAERLLGLEPSAVRRLGALAVAVTE
                     DARRLATRLRLSSAETKALDSMGHRWWRLAGMDEAIARRRLYRLGEERYRDRLMLAWA
                     RAGAGTDSAHWRELATLPLRWSAPKFPLKAADFIARGIAEGPALGQVLALAEDAWLAA
                     DFPLDEPALKAIADQTVARFSRDHRP"
     gene            complement(2333..2596)
                     /locus_tag="VN968_02900"
     CDS             complement(2333..2596)
                     /locus_tag="VN968_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HXL28232.1"
                     /translation="MIRPALTEIGIFLIPFAVYALFLIATRSELLIQSSWPVHIIAKL
                     VLGSLLLVVLSFILLAHFSGAPPNSTYIPAHIENGKFVPGVEK"
     gene            complement(2593..3264)
                     /locus_tag="VN968_02905"
     CDS             complement(2593..3264)
                     /locus_tag="VN968_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HXL28233.1"
                     /translation="MNEPMLKTDPAAIGSVEFFDRARARLRFDIPAGLTDASVVPRSG
                     DEGTDRMLQIIAREQPIRPAAVLIPVVGHAQPTVLLTQRAAHLNDHAGQISFPGGKID
                     ATDASPLEAAIREAEEEIGLAREFIDPIGYLDLYATGFGYRILPTVARVKPGFKLRIN
                     EAEVDDTFEVPLAFLMDPVNHQVHSKEFRGIERSYYAMPFEERYIWGATAGILRVLYE
                     RICLP"
     gene            complement(3261..3869)
                     /locus_tag="VN968_02910"
     CDS             complement(3261..3869)
                     /locus_tag="VN968_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HXL28234.1"
                     /translation="MAKQGQTTHQGLEGLTAAVRNAANAGPAKGLPPVHLWNPPFCGD
                     LDMRIASDGTWFYMGTPIGRPALVRLFSTILKREDGKHFLVTPVEKVGIRVDDAPFLA
                     VEMLKEADDRGRLLRFRTNADDWVPCDSGHRLRFEAAADGGLTPYLHVRADLWAKVTR
                     ALYYDLVDMGEERVVDGRPMFGIASAGEFFAMADAEQVRGAL"
     gene            4113..5117
                     /locus_tag="VN968_02915"
     CDS             4113..5117
                     /locus_tag="VN968_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXL28235.1"
                     /translation="MAGADSVEKLEDVIVRSAEQVAGQIRAAKDAISTVIFGQDRVIE
                     NTLVTILSGGHALLIGVPGLAKTKLVETLGITLGLDAKRIQFTPDLMPSDILGAEVLD
                     ESTSGKRSFRFISGPVFAQLLMADEINRASPRTQSALLQAMQEQHITVAGARHDLPKP
                     FHVLATQNPLEQEGTYPLPEAQLDRFLMEIDVDYPDRDAERRILFETTGADETLAKAA
                     MNADILITAQRLVRRLPVGDSVVEAILSLVRSARPGPEGGEAGKLIAWGPGPRASQSL
                     MLAVRARALLDGRLAPSIDDVLDLAEPILKHRMALTFSARAEGRTIPDVIRQLKTRIG
                     "
     gene            5117..6061
                     /locus_tag="VN968_02920"
     CDS             5117..6061
                     /locus_tag="VN968_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXL28236.1"
                     /translation="MAAETRHTTQEIVAIRRADGESRTLAASLPRLVLEARRIAANVI
                     HGLHGRRRAGAGESFWQYRRFVSGEPSQSVDWRRSARDDHLYVREQEWEAAHTVWLWP
                     DRSPSMAFASKQARDSKLERGLIVTFALAELLVAGGERVGVPGLMTPTASRSVIDKMA
                     QAMLHDDAARLSLPPSFVPSALAEIVVLSDFWSPIGEIRTMLAGLSASGAHGTLVQIV
                     DPAEESFPYSGRIEFVEPEGGGVITAGRAEKWADDYVTRVARHRDEIRSETNKLDWLF
                     STHTTSRSAAELLLFLHSGMMVSKGSARFATVKAGRSA"
     gene            6061..8868
                     /locus_tag="VN968_02925"
     CDS             6061..8868
                     /locus_tag="VN968_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HXL28237.1"
                     /translation="MAGLPLTFAEPFLLLGLLSLPVLWWLLRVMPPRPRRIEFPPTRL
                     LLDIAPKEETPSHTPWWLTALRLAAATLVILAAAGPIWNPQTGLAGNSAPLVLLLDDG
                     WSAASSWDIRIKAADELIANADSNRRGVALVPLSEPARDITLMPGGTARVALRQLVPK
                     PYSIERVETLPAIERFLKATGDSEIAWLSDGVDTGRGPEFLEGLSKTIADRALTVFEG
                     GTPSPLALVAAENAAAKMTVKVLRADGGVAAGIVRAMDPKGSPIGEARYTFSPRDRET
                     EAAFDLPVELRNDIARLEISGERSAGAVQLLDKRWRRRAIGIVSGSTNDTAQPLLAST
                     FYLTRAMAPFADVRLGDRGAPQQAITQFLDQKLPMIVLADVGTLSPEIRERIAAWIEQ
                     GGVLVRFAGPRLAQADDDLVPVKLRRGGRSLGGSLTWEKPQHLASFAADGPFAGLPVP
                     KDITVSRQVLAEPDAVLATKSWASLEDGTPLVTGEHRGKGLVSLFHVSADMRWSDLPM
                     SGSFVEMLRRIVDASGYTSNPGAGVAGEAKVETVAPLRTLDGFGAFGPPPSTAKPLPA
                     DFRDRAAPDHPPGFYGPADGPLAVNALAAADRIAPLDTAGLRARRASYTNAEPRDLRG
                     ILLSSSLALFLIDAIIVAVLGAGIAALLRRRAAPAALAFALVLSTILAAPSPSRADNN
                     DDFAIKSVSQTRLAYVVTGNTDVDSIVKAGMSGLTLFLAQRTALEAGDPVGIDPARDE
                     LAFFPLIYWPVVPGAAKPPQDAINRIDAYMKQGGTVVFDTRDAIEAPPGESGASQTQG
                     MLALRNILSSLDVPELEPVPREHVLTKTFYLLHDFPGRFNSGQTWVETLPRDEDEEAA
                     SRPARGGDGVSPIIITSNDLAGAWAIRPDGQPMLPLTPGEPKQREFAFRAGVNIVMYT
                     LTGNYKADQVHAPALIERLGQ"
     gene            8878..10941
                     /locus_tag="VN968_02930"
     CDS             8878..10941
                     /locus_tag="VN968_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28238.1"
                     /translation="MQYGIAFTPLVPSLVLWLALAAIAVIAVLLLLGRARGAAVRVTA
                     LALILLALANPSFTREDREPLSSVAAVVIDKSPSQNFGERNRETAKAQEALVDSLKTI
                     KGLEVRVVEAGQADGETDGTRLFGALSSTLSDVPVDRVAGAFLITDGRVHDIPANAAG
                     LGFQAPVHALITGRKDERDRRIAISAAPRFGIVGQVQTITYRLDDQGVSGDRAKIVVR
                     RDGEVINERTVQSGQTVNVEIDIKHAGPNIVEIEASPLDNELTLVNNRAVVAIDGVRD
                     KLRVLLVSGEPHSGERTWRNLLKSDASVDLVHFTILRPPEKQDGTPINELSLIAFPTR
                     ELFQQHINEFQLIIFDRYARQGVLPIAYFDNIARYVRAGGAVLVSAGPDYASTTSIWR
                     TPLDSVLPAEPVGVTEKPFYAHLSDAGKRHPVTRGLDGSASEPPHWSRFFRTVETRNA
                     TSPPVMTGADGKPLLLLSRFGEGRVALLLSDHIWLWARGYEGGGPHLDLLRRMSHWLM
                     KQPDLDEEALRLQVQGKDLVVLRQTMADSVTPVTVTSPSGATRELTLSASEPGTWRST
                     IPANELGLWQATDGTLKALINVGPTNPKEFSEVTSTTEMLKPLTQATGGDTRRVVDGS
                     SIELPRIVPVRASGVFRGDGWMGVKMRDASVVRGVGVLPVFAGLIGLLLLLGAFAATW
                     LREGR"
BASE COUNT         2033 a         3647 c         3654 g         1941 t
ORIGIN      
        1 ctagccatcg cgtcaatcaa cttggggaaa gaaatcatgg acttgaagcc aggcgatgtt
       61 gtcattctga aatccggagg tcattcgatg accgtggcgg aagtcaagga cggcgccatc
      121 gcatgcgtct ggatgggcgg agaaggcgat ctgtttcgcg aaacgctttc atcagccgtg
      181 ctcgaacagg tcgaagtcga ggattcggac gacgaagagg aagacgaaga agaggacgag
      241 gaagaggtcg catgacttcg cttgcgatgc cgccgaagct tgggcccggc ggcatcgctt
      301 tttcggtaat ttcgttcaat gaaaggatcg cgccgcttcg acaatcatga ccgtcccgcc
      361 cgcgatcacg gcgacatcga ggatcgcggc gtgaacatgc accggcatgc gctcgacaaa
      421 ggcgcgggcg agaaacgccc cggggagcgc aatcgcgccg atcagaacgg caaaggcgaa
      481 cacttgcgcg gtgaccacgc cagccagacc gaatactgaa atcttgatga tgctggtggc
      541 gaccgagatc acggcatcgg tggcgatcac ggcggcgccc tcgaggccgg ccgccatcag
      601 gagcgacagc agaatgacgc ccgggcccga ggtgccacca accacgacgc cgtagcccac
      661 cgagcccgcg gccagcccgg tgtcactgat tttgatatcg cggcgtttga gcagacagcg
      721 catcggcacg ctcgatatca gcatggcgcc gatcgccagc gcggcgccgg cgttggtaag
      781 ccgggtatag ccgtaagcgc cgagcgctgt cgtcaatgcg gccgccgcaa tcacgattgc
      841 cgcgcggcgg cggtcggcat agcggagata ggcggcgaaa cggctggtgt tggtaaagat
      901 cgaggagatg gcgatgatcg gcaccactgg ttcggcgccg atcaggggca ccagaaccag
      961 cggcatcagc gcgccggtgc cgtagcccgc caacccgccg acaacagagg caaacagcgc
     1021 gacgcacgcc accagcagga gttggttcgc ggaaatatcg gcgaagccgg aaatgatact
     1081 cacggccgat ggtcgcggct gaaacgggcg acggtctggt cggcaatggc tttgagcgca
     1141 ggctcatcca gtgggaaatc cgccgccagc cacgcgtctt cggccagcgc gagcacctgc
     1201 ccgagcgcgg gaccttcggc aattccgcgc gcgatgaaat cggctgcctt cagcggaaat
     1261 ttcggcgcac tccagcgcag cggcagggtg gcgagttcgc gccaatgcgc cgaatcggtg
     1321 cccgctcctg cccgcgccca cgccaacatc agccggtcgc ggtaacgctc ctcgccgagc
     1381 cggtagagcc gtcgccgtgc gatcgcctcg tccatgccgg cgagccgcca ccagcgatgg
     1441 cccatggaat cgagcgcctt ggtttcggca ctgctcagcc gcaagcgcgt cgcaagccgc
     1501 ctggcgtcct cggtgacggc gaccgccagc gcgccgaggc ggcgaaccgc gctcggttca
     1561 agtccaagca ggcgctccgc cgatatcatg gctgtaaacg gtcctatata ggcgacaccg
     1621 ccgaaaatcg gcagcagtaa cccgccatcc gccattgctg cgaccgcgcc gggcgcgcct
     1681 tccgcgacca tcagtttcag catttccatg cgcacgcgtt cggcagagag ggtcgtaagg
     1741 cctgctcgcg ccgcgatgca ggcaaggtag ccggcgcgat cgggctcacc ggcgccatag
     1801 gcggcgtgaa tgcggaaaaa cctggggatg cgcagatagt cttcggcgat acgctggctc
     1861 ggatcgccga tgaagcgcac ccgtttggcg aggacatcat ccaacccgcc gacatggtca
     1921 tgcaccacac caccggcgtc aacggacagg ccgttgatgg tgaagtcgcg ccgctccgcg
     1981 tcgcgaaccc agtcgcgccc gaacgcgacc ttcgccttgc ggccgaacgt ctcggtatcc
     2041 tcgcgcaagg tggtgacctc aaacggctgc gcgtcgacga ccagcgtcac ggtgccatgt
     2101 tcgatgccgg tcggcacgct cttgatgccg gccgccttgg cgcggcggat cacttcatcg
     2161 ggcacggcgg tggtcgcgat atcgatatcg ccggtcggaa tcttcaagag cgcgttgcga
     2221 accgcgccgc cgaccacccg cgcctcctcg ccgttaccgt tcagcatttc gagcacgcgg
     2281 acggccggac cggatttgaa ccagggcgcg tcgtcgagca cgcgcgcgtc agtcattttt
     2341 caactccggg aacgaacttg ccgttctcga tatgcgcggg aatgtaagtc gaattcggcg
     2401 gcgcgccgga aaaatgcgcg agcaaaatga agctgagcac caccagcagc aatgaaccga
     2461 gcaccagttt ggcaatgata tgcaccggcc atgatgactg aatcaacaat tcggagcgtg
     2521 tcgcgatcaa aaacagcgca tagaccgcga acgggatcag gaaaattccg atctcggtta
     2581 aagccggccg gatcatggca ggcagatccg ctcatacagc acgcgcagaa tccccgcggt
     2641 cgcgccccag atatagcgct cctcgaacgg catggcgtag tacgaacgct cgatgccgcg
     2701 gaattccttg ctgtgcacct gatggttcac agggtccatc aggaacgcca gcggaacctc
     2761 gaacgtatcg tcgacttcgg cttcgttgat gcgcagcttg aagcccggtt tcacccgcgc
     2821 aaccgtcggc aggatgcgat atccgaaccc ggtcgcgtac aaatcgagat agccgatcgg
     2881 atcgatgaat tccctggcaa ggccgatctc ctcctcggcc tcacgtatcg ccgcctcgag
     2941 cggcgaggcg tcggtcgcgt cgatcttgcc gccggggaag gagatctggc cggcgtgatc
     3001 gttgagatgt gccgcgcgct gggtcaacag cacggtcggt tgcgcatgcc ccaccaccgg
     3061 gatcaacacc gcggctgggc ggatcggctg ttcgcgggcg atgatctgca gcatccgatc
     3121 ggtaccctcg tcgccgctcc ggggaaccac gctggcgtcg gtcaaccctg cgggaatgtc
     3181 gaagcgcagc cgcgcgcggg cccgatcgaa gaactccact gagccgatcg cggcagggtc
     3241 ggtcttcagc atcggctcgt tcaaagcgcg cccctcacct gctccgcatc cgccatcgca
     3301 aagaattcgc cggccgacgc gatcccgaac attggacgac catcgaccac tcgctcctcg
     3361 cccatgtcaa ccagatcata ataaagcgcc cgtgtgacct tggcccacag gtcggcgcgg
     3421 acgtgaagat agggcgtgag gccgccgtcg gccgcggcct cgaatcgcag ccggtgcccg
     3481 gaatcgcatg gcacccagtc gtcggcgttg gtgcggaagc gcagcagccg gcctcgatca
     3541 tccgcttcct tgagcatctc aaccgccaga aacggcgcat cgtcgacgcg gatgccgacc
     3601 ttctccaccg gggtaacgag aaagtgcttg ccgtcctcac gcttgagtat tgtggaaaac
     3661 agccggacca aagcgggtcg tccgatcggc gtacccatgt agaaccatgt accatcgctg
     3721 gcgattcgca tgtcgagatc gccgcagaac ggcggattcc acaaatgcac gggcggcagc
     3781 ccttttgcgg gaccggcgtt tgcagcgttc ctgacggcag cagtcagtcc ttcgaggccc
     3841 tgatgggtgg tctgcccttg cttcgccatt gtttaccctg aggttgattg cctcgatctg
     3901 gcacgattgg tgcacgtctt ccggtgtgtg taggcgagtt ccctcgggtg aaaccctgag
     3961 gtcaagtcgc cacatcgtga tacagttcat acccctaaat accgataatg tggggatagt
     4021 ttaactcaac gaatacatgg ccttcgcata agtttatgca ggggtgcatg acatgacgtc
     4081 gcaatcggcg gcgtttggac aaggagtgac caatggctgg cgcagacagt gtcgaaaaac
     4141 ttgaggacgt gatcgtccgc tcggccgaac aggtcgccgg ccaaatccgc gccgcgaagg
     4201 atgctatctc cacagttatc ttcggtcagg accgggtgat tgaaaatacc ctggtgacga
     4261 tcctgtccgg cggtcacgcg cttctaatcg gtgtgcccgg tctcgccaag accaagctgg
     4321 tggaaacctt gggcattacg ctcgggctcg acgccaagcg catccagttc acgccagacc
     4381 tgatgccctc ggatattctg ggcgcggagg tgctggacga gagcacttcc ggcaagcggt
     4441 cgttccgctt catctccgga ccggtgttcg ctcagcttct gatggccgac gaaatcaacc
     4501 gcgccagccc gcgcacccag tccgcgctgc tgcaggcgat gcaggagcag cacatcacgg
     4561 ttgcgggcgc gcgccacgat ttgccgaagc cgttccatgt gctcgcgacc caaaacccgc
     4621 tggagcagga aggcacctac ccactgccgg aggcccagct cgatcgcttt ctgatggaga
     4681 tcgacgtcga ctatcccgat cgcgacgccg agcggcgcat cctgttcgaa accacgggcg
     4741 cggacgagac gctcgccaag gccgccatga acgcggatat cctaatcacc gcgcaacgat
     4801 tggtgcgccg actgccggtc ggcgactccg tcgtggaagc gatcctctcg ctggtgcgct
     4861 ccgcgcgtcc gggtccggaa ggcggcgagg ccggcaagct gatcgcctgg gggccgggtc
     4921 cccgcgccag ccagtcactg atgctcgcgg ttcgtgcgcg cgcattgctc gacggccgcc
     4981 tggcgccttc gatcgacgac gtgctcgatc tggccgagcc gattctcaag caccgcatgg
     5041 cgctgacgtt ttccgcccgt gccgaaggcc gcaccattcc tgacgtgatc cggcaattga
     5101 agacgcggat tggttgatgg ccgcggagac caggcacaca acccaggaga tcgtagcgat
     5161 ccgacgtgcc gacggcgaaa gccgaacgct cgccgcatcg ctgccgcgcc ttgtgcttga
     5221 agctcgccgc atcgccgcca acgtcattca cggcctgcac gggcggcggc gcgccggcgc
     5281 cggggaaagc ttctggcaat atcgccgctt cgtttcgggc gagccatccc agagcgtcga
     5341 ctggcggcgc tcggcgcgcg acgatcatct ctatgtccgc gagcaggaat gggaggccgc
     5401 gcacaccgtg tggctgtggc cggaccgctc gccgtcgatg gcgttcgcat ccaaacaggc
     5461 gcgcgacagc aagctggagc gcgggttgat cgtgaccttc gcgctggccg aactgctggt
     5521 cgcgggcggc gaacgggtcg gcgttcccgg cctgatgacc ccgaccgcaa gccgcagcgt
     5581 gatcgacaag atggcgcaag cgatgctgca cgatgatgca gcccgcctga gcctgccgcc
     5641 gtcatttgtg ccgtcggcgc tggccgaaat cgtggtgctg tcggatttct ggtcgccgat
     5701 cggcgaaatc aggaccatgc tcgccggatt atcggcatcc ggcgcgcacg gcaccctggt
     5761 gcagatcgtg gacccggcag aagaaagctt cccctattcc ggccggatcg agttcgtcga
     5821 gccggaaggc ggcggcgtca tcaccgcggg ccgcgcggaa aaatgggcgg acgactacgt
     5881 tacgcgggtc gcacgtcacc gtgacgagat tcgcagcgag accaacaagc tggactggct
     5941 gttctcgacc cacaccacca gccgctcggc cgccgaactg ctgctgttcc tgcacagcgg
     6001 catgatggtg agcaagggaa gcgcgcgctt cgcgaccgtc aaggcggggc gaagcgcatg
     6061 atcgcggggc tgcctctcac tttcgccgaa ccatttctgc tgctgggtct gttgagcctg
     6121 cccgtgctgt ggtggctgtt gcgggtaatg ccgccgcggc cgcggcgcat tgagtttccg
     6181 ccgacgcggc tgttgctgga catcgcgccg aaggaagaaa ccccgtcgca tacaccatgg
     6241 tggctgacgg cgttgcggct tgcagccgcg acgctggtga tcctggccgc cgcggggccg
     6301 atctggaatc cgcaaaccgg ccttgccggc aatagcgcgc cgcttgtcct cctgctcgac
     6361 gacggctgga gcgcggcctc gagttgggac atcaggatca aggccgcgga cgaattgatt
     6421 gcgaatgccg acagcaaccg gcgcggcgtt gcgctggttc cgctgtcgga gcccgcgcgc
     6481 gacatcacac tgatgccggg gggcaccgcg cgcgtggcgc tgcgccagct tgtgcccaag
     6541 ccctactcga tcgaaagggt cgaaaccctg cccgcgatcg agcgtttcct caaagccacc
     6601 ggcgacagcg agatcgcctg gctgtcggat ggcgtcgata ccggacgagg gccggagttt
     6661 ctcgaagggc tgagcaagac aatcgcggat cgtgcattga cggtttttga aggcggcact
     6721 ccatccccgc tcgctttggt cgccgccgag aacgccgcgg cgaagatgac tgtgaaggtg
     6781 ctgcgcgccg atggcggcgt tgccgcgggc atcgtgcggg cgatggatcc aaaaggctcg
     6841 ccgatcggcg aagcccgcta tacgttttcg ccacgtgatc gcgagaccga agcggcgttc
     6901 gatcttccgg tggagctgcg caacgacatc gcgcgcctcg aaatatcggg cgaacgatcg
     6961 gcgggcgcgg tgcaactgct cgacaagcga tggcgccggc gcgccatcgg catcgtcagc
     7021 ggatcgacca acgataccgc gcagccgctg ctggcatcga cattctacct cacccgggcg
     7081 atggcgccgt tcgccgacgt gcggctgggc gatcgcggcg cgccgcaaca ggcgatcaca
     7141 cagttcctcg accagaagct gccgatgatc gtgctcgcgg atgtcggcac gctgtcgccc
     7201 gaaattcgcg agcgcatagc tgcgtggatc gagcaaggcg gggtgctggt gcgttttgcc
     7261 ggcccgcgtc tggcgcaggc ggatgacgat ctggttccgg taaagctgcg ccgcggcgga
     7321 cgcagcctcg gcggcagtct tacctgggaa aagccgcagc accttgcctc gttcgcggcc
     7381 gatggaccgt tcgccggtct gccggtgccg aaggacatca ccgtcagccg gcaggtgctc
     7441 gccgaaccgg atgcggtgct ggcgaccaag agctgggcct cgctggagga tggaaccccg
     7501 ctggtgacgg gcgaacatcg cggcaagggc ctggtgagcc tgtttcatgt cagcgccgac
     7561 atgcgctggt ccgatctgcc tatgtccggc agctttgtcg agatgctgag gcggatcgtc
     7621 gacgcatcag gctatacgtc caatccgggt gccggcgtgg cgggcgaagc caaggtcgaa
     7681 accgtagcgc cgttgcggac gctggatggt ttcggggcgt tcggaccacc gccctcaacc
     7741 gcgaagccgc tgccggccga cttccgggac cgggcggcac cggaccatcc gcccggattc
     7801 tatggccccg ccgacggtcc tctcgcggtc aatgcgctgg cggcggcgga tcgcatcgcg
     7861 ccgctcgata ccgcgggcct gcgcgcgcgg cgtgccagct acaccaatgc cgagccgcgc
     7921 gatctgcgcg gcatcctgct gtcgtcgtca ctggcgctgt tcctgatcga cgcgattatc
     7981 gtcgcggtgc ttggcgccgg catcgcagcg ttgctgcggc gccgcgccgc gcctgctgcg
     8041 ctggcgttcg cgttggtatt gtccaccata ttggcagccc catcgccgtc ccgtgccgac
     8101 aacaacgacg atttcgcgat caagtcggtc tcgcaaaccc gcctcgccta tgtcgttacc
     8161 ggcaataccg acgtcgattc catcgtcaag gcagggatgt ccgggctgac gttgtttctc
     8221 gcgcagcgca cggcattgga ggccggcgac cccgttggca tcgaccctgc acgcgacgaa
     8281 ctggcgttct tccccctgat ctactggccg gttgtgcccg gcgcggcgaa gccgccgcaa
     8341 gacgccatca accgcatcga cgcctacatg aaacagggcg gcaccgtggt gttcgacacg
     8401 cgcgatgcca tcgaggcgcc gccgggagaa agcggcgcat cgcagacgca gggaatgctg
     8461 gccttgcgca atattctctc ctcgcttgac gttcccgagc ttgaaccggt tccgcgcgaa
     8521 cacgtgctga ccaagacgtt ctatctgctg cacgatttcc ccggccggtt taactccggc
     8581 cagacctggg ttgaaacctt gccgcgtgac gaggacgagg aggccgcctc ccggccggcc
     8641 cggggcggcg acggcgtctc accgattatc atcacttcga acgatctcgc cggtgcctgg
     8701 gcgatacgcc ccgacggcca gccgatgctg ccgctgacac cgggcgagcc caagcagcgc
     8761 gaattcgctt tccgcgccgg cgtcaacatc gtgatgtata cgctgaccgg aaactacaag
     8821 gccgaccagg tgcatgcgcc ggcgctgatc gaacggctcg gacagtagga acgcaatatg
     8881 cagtacggca tcgcgtttac accgctcgtc ccctcgctgg tgctgtggct cgcgctggca
     8941 gccatcgccg tgatcgccgt cctgctcctg ctcggccgcg cgcgcggcgc ggcggtgcgc
     9001 gtgacagcgc tggcgcttat cctgctggcg ttggccaatc cgtcatttac ccgcgaggac
     9061 cgcgagccgt tatcgtcggt ggccgctgtc gtgatcgaca agagccccag ccagaatttc
     9121 ggcgagcgca accgcgaaac cgcgaaagcg caggaggcat tggtcgatag cctcaagacc
     9181 atcaaggggc tggaggtgcg ggtcgtcgaa gccggccagg ccgacggcga aaccgatggc
     9241 acgaggttat tcggcgcgct gtcgtcgacg ctgtcggacg ttcccgtcga tcgcgtcgct
     9301 ggtgcatttc tgattaccga cggccgggtg catgacattc ctgccaacgc ggcggggctt
     9361 ggatttcagg ccccggtgca tgcgctgatc acgggacgca aagacgagcg cgaccggcgc
     9421 atcgcgattt ccgcagcccc ccgcttcggc atcgtcggac aagtccagac catcacctac
     9481 cggctggacg atcaaggcgt cagcggtgac cgcgccaaga ttgtggtgcg tcgcgacggc
     9541 gaagtgatca acgagcgcac cgtgcagagc gggcagaccg tcaacgtcga gatcgatatc
     9601 aagcacgccg ggcctaacat cgtcgagatc gaggcctcac cgctcgataa tgagctgaca
     9661 ctggtcaaca accgcgcagt ggttgcgatc gacggcgtgc gcgacaagtt gcgggtgctc
     9721 ttggtctcgg gcgagccgca ttccggggaa cgaacctggc gcaacctgct gaagtccgac
     9781 gccagcgtcg atctcgtgca ttttacgatt ttgcgccccc ctgaaaagca ggatgggacg
     9841 ccgatcaacg aattgtccct gatcgcgttt ccgacccgcg aactgttcca acagcatatc
     9901 aacgaatttc aattgatcat ctttgaccgc tatgcgcgcc agggcgtgct gccgatcgcc
     9961 tacttcgaca acatcgcgcg ctacgttcgc gccggcggcg cggtgctggt gtcggcaggt
    10021 cccgattacg cctcgaccac cagtatctgg cgcacgccgc tggattcggt tttgccggcc
    10081 gagccggtcg gtgttaccga gaagccgttc tacgcgcatc tgagcgacgc cggcaaacgc
    10141 catccggtga cgcgcgggct ggacgggtcc gccagcgagc cgccgcactg gagccggttc
    10201 ttccgtactg tcgagacgcg caatgcaacc agcccgccgg tcatgaccgg cgccgacggc
    10261 aaaccgctgc tgctgctgtc gcggtttggc gagggccgcg tcgcattgct gctgtcggat
    10321 cacatctggt tgtgggcacg cggctatgag ggcggcggac cgcatctcga cctgctgcgg
    10381 cgcatgtcgc attggctgat gaagcagccc gatctcgacg aagaggcgtt gcgcctgcag
    10441 gtccagggta aggacctggt ggtgctgcgc cagaccatgg ccgacagcgt cacgccggtg
    10501 acggtgactt cgccgtcggg tgcgacgcgc gagctgacgc tgagcgccag cgaacccggg
    10561 acgtggcgct cgacgatccc cgccaacgaa cttggtctgt ggcaggcgac cgatggcacg
    10621 ctgaaagcct tgatcaatgt cggcccgacc aatccgaagg agttttcgga agttacctcc
    10681 accaccgaaa tgctgaagcc gctgacgcag gccaccggcg gcgacacccg gcgcgtcgtg
    10741 gatggctcca gcatcgagct tccccgcatc gtgccggtgc gcgcatccgg ggtgtttcgc
    10801 ggcgacggct ggatgggggt gaagatgcgc gatgcgagcg tggtcagagg cgtcggcgtg
    10861 ctgccggtgt tcgccggcct gatcggcctg ctgctgctgc tcggcgcatt cgccgccacc
    10921 tggctgcgcg aggggcgttg atcgcacctc gtgtcgcccg gcgttgcgtg aatcacacag
    10981 accctgcgaa ttctgccggc acgcatatgg accgcggttg acactcttca ccggttcctg
    11041 cgatgttaca ttataacatc ggggtggccg ggatatctcg gccttccgac gcaaggcgca
    11101 tcgctggatg aagtggttcc gatcgaacat caaacatggc tcgcggctcg cgctgtttgc
    11161 gctggcgatc cagtttgcat tgtcgttcgg acatttccac gcggtcgcgg cacaagcgat
    11221 tcagccggac gctgcgccgt tcgaactttc ctacgccaac cgcctcaccg cgccg
//
LOCUS       DATNOI010000084         4591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2108097,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000084 DATNOI010000000
VERSION     DATNOI010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2108097"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VN968_02935"
     CDS             <1..508
                     /locus_tag="VN968_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HXL28239.1"
                     /translation="FDPAEKLKGIRIGLAEYRRPDPRTIPAMAKAAGLYMICTISKHK
                     AERKGYADAMMLDWQGRIAECTGANIFFIKDGKIHTPTADCFLDGITRQTVIALARRR
                     GIEVAERRIMPDELTGFSECFITGTAAEITPVSEIAKWRFTPGAISEQLMADYTAEVQ
                     PKGKAAAA"
     gene            690..2192
                     /gene="hisS"
                     /locus_tag="VN968_02940"
     CDS             690..2192
                     /gene="hisS"
                     /locus_tag="VN968_02940"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HXL28240.1"
                     /translation="MAEKPKKPQKLRARLPRGLADRGPAEIAATRRMTETIREVFERY
                     GFEPVETPAMEFTDALGKFLPDQDRPNEGVFSFQDDDEQWISLRYDLTAPLARYVAEN
                     FDSLPKPYRSYRAGYVYRNEKPGPGRFRQFMQFDADTVGSASPAADAEMCMMAADTME
                     ALGIPRGSYVVKVNNRKVLDGVMESIGLGGDENAGRRLTVLRAIDKLDRLGWENIISL
                     LKSGRKDESGDYTKGADLTDVQVVQLQTFIFTKFENLTHAKSLFDNSTYQAGIDELDE
                     IWRLAAASGYDDSRIGIFPSVIRGLEYYTGPVYEVELSLLATDEKGRIVQFGSVGGGG
                     RYDGLVSRFRGEPVPATGFSIGVSRLQAALTMLGKLDTAPEFGPVVVTVFDRERVADY
                     QKMVAALRAANIRAELYLGNPKSMGNQLKYADKRNSPCVIIQGSDEKDDAAGPQVLLK
                     DLILGAELAKLEKDRDEYLQKQGEAQTKVPQAALVDEVRKLLDKHGVKWK"
     gene            2343..2546
                     /locus_tag="VN968_02945"
     CDS             2343..2546
                     /locus_tag="VN968_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HXL28241.1"
                     /translation="MPEITVSMAAGRTDEQKAGMMRDITQALVKNLGVDADAVVIQIN
                     EAPLAHKMKGGKTFVERAAVQKK"
     gene            2713..3108
                     /locus_tag="VN968_02950"
     CDS             2713..3108
                     /locus_tag="VN968_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXL28242.1"
                     /translation="MDARNVIKLGMSAERTLKVTPDLTVRHIIPTMPAVFATPMMILE
                     MELTSGDAINAYLEPGWITVGTEVDIRHLAASAVGAVIRTTARVIAVARRVIRFEVEA
                     FDGERRIGEGRHARGLVNLATFNKQLGSN"
     gene            complement(3236..4069)
                     /gene="proC"
                     /locus_tag="VN968_02955"
     CDS             complement(3236..4069)
                     /gene="proC"
                     /locus_tag="VN968_02955"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HXL28243.1"
                     /translation="MTSNTTNALQDIKTTIVLAGAGKMGGALLSGWLAQGLDGKRVVV
                     IEPQPSDEISALVTKGVRLNPSPKDSGAVATLVIALKPQSFREAGAMLKSFTGPSTLV
                     VSIMAGTTIASIAEACGGSVVRAMPNTPAAIGRGITVAVAAGDVSDSQRAIADALLRA
                     TGSVEWVDDENLMDAVTAVSGSGPAYIFLLAEELARAGVEAGLPQELATRLARETVAG
                     SGELLRLSEAPSATLRQNVTSPGGTTAAALEVLMGPDGMQSLLTRAVAAATRRSKELA
                     K"
     gene            complement(4255..>4591)
                     /locus_tag="VN968_02960"
     CDS             complement(4255..>4591)
                     /locus_tag="VN968_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HXL28244.1"
                     /translation="TEIEALHLACAFDMKIPAARRAEVQRLIAAINEQLWVGHFDIWT
                     HTGMIMYRQALVLPGGITASTAQCETMLVGAIHACERYYPAFQFVVWAGKSAPEAISA
                     AMFDTEGEA"
BASE COUNT          916 a         1429 c         1470 g          776 t
ORIGIN      
        1 cttcgatcct gccgaaaagc tgaagggcat ccgcatcggc ctcgccgaat atcgccggcc
       61 cgatccgagg acgatcccgg cgatggcgaa ggcggccggc ctctacatga tctgcaccat
      121 ctccaagcac aaagccgagc gcaaaggcta tgccgacgcc atgatgctcg actggcaggg
      181 ccgcatcgcc gaatgcaccg gcgccaacat cttcttcatc aaggatggca agattcatac
      241 gcccactgcc gactgcttcc ttgatggcat cacgcggcag accgtgatcg cgctggcgcg
      301 acggcgcggg atcgaggtgg ccgagcggcg catcatgccg gacgagctga ccggattttc
      361 ggagtgcttc atcaccggaa ccgccgcgga aattacgccg gtgtcggaga tcgccaagtg
      421 gaggttcacc cccggcgcga tctcggagca gctgatggcg gactacaccg ccgaagtgca
      481 gccgaagggc aaggcggcgg cggcgtaacc accgcggcgc gccctcactt ctccctctcc
      541 cgcgctttgc gggggagggc cggggtgggg gaaagccaca agcaccgggg cccgcgtcgc
      601 ccccacccca accctccccc gcaaagagcg ggagaggggg aagcaagccc ggttgccgca
      661 tcgcgcctcg gctggtacca acgccgccca tggccgagaa accgaaaaaa ccccagaaac
      721 tgcgcgcgcg cctgccgcgc gggcttgccg atcgcgggcc tgccgagatc gcggcgacgc
      781 ggcgcatgac cgaaaccatc cgcgaggtgt tcgagcgcta cggttttgag ccggtggaaa
      841 ccccggcgat ggaattcacc gacgcgctcg gcaaattcct gcccgaccag gaccggccca
      901 atgagggcgt gttctcgttc caggacgacg acgagcagtg gatttcgctg cgctacgatc
      961 tgacggcgcc gctggcgcgc tatgtcgcgg aaaatttcga ttcgctgcca aaaccctacc
     1021 gcagctaccg cgccggttac gtctatcgca acgagaagcc gggccccggc cgcttccgcc
     1081 agttcatgca gtttgatgcg gataccgtgg gcagcgcgtc gccggccgcg gatgccgaga
     1141 tgtgcatgat ggcggcggac accatggaag cgctgggcat cccgcgcggc agctatgtcg
     1201 tgaaggtgaa caaccgcaag gtgctcgatg gggtgatgga gagcatcgga ctcggtgggg
     1261 atgagaatgc gggtcggcgg ctaacggtgc tgcgggcgat tgataagctg gatcggctag
     1321 gatgggaaaa tattatttcg ctgctcaagt caggtagaaa agacgaaagc ggtgattaca
     1381 caaaaggcgc cgatctcact gacgttcaag tagttcaact gcaaactttc attttcacca
     1441 agtttgaaaa tttaacgcat gcaaaaagtt tgttcgacaa ttccacctat caagctggca
     1501 tagatgagct ggatgagatt tggcgtctcg cagctgcctc aggatatgac gacagtcgca
     1561 taggcatctt cccctccgtc atccgcggcc tcgagtacta tacgggtccc gtttatgaag
     1621 ttgagttgtc gttgctcgct accgacgaaa agggtcgtat agtacagttc ggttcggtcg
     1681 gcggcggcgg acgctatgat ggactcgtgt cgcggtttcg cggcgagccg gtgccggcga
     1741 cgggattttc cattggcgtg tcgcgattgc aggccgcgct caccatgctc ggcaagctcg
     1801 ataccgcacc ggagttcggc cccgtcgtgg tcaccgtgtt cgatcgcgag cgcgtcgccg
     1861 actatcagaa gatggtcgca gccttgcgcg ccgccaacat tcgcgccgag ctttatctcg
     1921 gcaaccccaa gagcatgggc aaccagctca aatatgccga caagcgcaac agcccctgcg
     1981 tgatcattca gggctctgat gagaaggacg atgccgcggg tccgcaggtg ttgttgaagg
     2041 acctcatcct cggcgccgaa ctggcaaaac tcgaaaaaga tcgcgacgaa tatctgcaga
     2101 agcagggtga ggcccagacc aaggtgccgc aggctgcatt ggttgatgaa gtacgcaagc
     2161 ttcttgataa gcacggggtg aagtggaaat agacctcatg gtgaggaggc gctcttgcgc
     2221 cgtctcgaac catgaggaca ataggcgcac atccttcgag acgcggccaa gaggccgctc
     2281 ctcaggatga ggatcgtgtt cgtggcgaga gtgacgtagc aagagcaaag ggagtgacaa
     2341 gaatgcctga aattaccgtg agcatggccg ccggccgcac cgatgagcag aaggccggca
     2401 tgatgcgcga catcacgcaa gcgctggtga agaacctcgg cgtcgacgcc gatgccgtcg
     2461 tgatccagat caacgaggcg ccgctggccc acaagatgaa gggcggcaag acgtttgtgg
     2521 agcgggcagc ggtgcagaaa aaatagcgcg taacctcatg ctcgtcgtcc cggcgaacgc
     2581 cgggacccat aacccctggc gtttccggta aagcgcaggg cgggccacga gcgcttcgcc
     2641 cgaacgctaa cggcacggcg tatgggtccc ggcgttcgcc gggacgacgc agtgatcatt
     2701 tcggatcgat ccatggatgc acgcaacgtc atcaaactcg gcatgagcgc cgagcgcacc
     2761 ctcaaggtga cgcccgatct caccgtcagg catatcatcc cgaccatgcc ggcggtgttc
     2821 gcgaccccga tgatgatcct ggaaatggag ttgacgtccg gcgacgccat caacgcctat
     2881 ctggaaccgg gctggatcac ggtcggcacc gaggtcgata tccgccatct cgcggcctca
     2941 gcggtcggcg cggtgatacg gaccaccgcg cgggtgatcg cggtggcgcg ccgggtcatc
     3001 cggttcgagg tcgaagcctt cgacggcgaa cgccggatcg gcgagggccg tcatgcgcgc
     3061 ggtctggtca atctcgcgac cttcaacaag cagttgggct ccaactagca aagccgcata
     3121 tatgcggacc tcatcctgag gagcgcgaag cgcgtctcga aggatggcaa cgcccgtggc
     3181 catccttcga gacgccgcgc cgaagcgcgg ctcctcagga tgagggtgga aaaaattact
     3241 tcgccagctc cttcgagcgg cgggtcgcag cggcgaccgc gcgcgtcagc agcgactgca
     3301 tgccgtccgg tcccatcagc acttccagcg ccgcggccgt ggtgccgccg ggggaggtga
     3361 cgttctggcg cagcgtcgcg gagggggcct ccgagagacg cagcaattcg ccggagccgg
     3421 cgacggtttc gcgcgccagc ctggtcgcga gctcctgggg caggccggct tcgacgccgg
     3481 cgcgcgccag ttcttcggcg agcaggaaaa tataggccgg accggaaccg gagaccgcgg
     3541 tcaccgcgtc catcaaattc tcgtcatcga cccattccac cgatccggtg gcgcgcaaca
     3601 gcgcatcggc gatggcgcgc tgcgagtcgc tgacatcgcc tgccgccacc gcgaccgtga
     3661 tgccgcggcc gatcgcggcc ggagtgttgg gcatggcgcg gacgacgctg ccgccgcatg
     3721 cctcggcgat cgaggcgatg gtcgtgcccg ccatgatcga cacgacaagc gtggaaggcc
     3781 cggtgaagga tttcagcatc gccccggcct cgcggaacga ctgcggcttc agcgcgatca
     3841 ccagtgtcgc gacggcgccg ctatccttcg gcgatggatt gagacgaaca ccttttgtca
     3901 ccagcgcgct gatctcatcg gagggctgcg gctcgatcac gacgacgcgc ttgccatcca
     3961 acccctgcgc cagccacccc gacagcaacg caccgcccat cttccccgcg ccggcgagga
     4021 cgatggtggt tttgatatct tgcaaagcgt tggtggtgtt cgaagtcatc ggaaacaaat
     4081 gcccacgtcg tcccggcgaa cgccgggacc cataaccacc agtgtttcta ttgaaacgaa
     4141 gaataggcca caagcacttt gcttgaacgc cggcgacacg gcgtatggat cccggctcgc
     4201 gctcgcgatg ctcgcttggc cgggacgacg ccgaaatttg cgctatgtga gaacttacgc
     4261 ctcaccctcg gtgtcgaaca tcgccgcgct gatggcttcc ggtgctgatt tgccggccca
     4321 caccacgaac tggaacgccg gataatagcg ctcgcaggcg tggatggcgc cgaccagcat
     4381 ggtctcgcac tgcgcggtcg aagcggtgat cccgcccggc agcaccagcg cctgccggta
     4441 catgatcatt ccggtgtggg tccagatgtc gaaatgcccg acccataatt gctcgttgat
     4501 cgctgcgatc agccgctgca cctcggcgcg gcgggctgcg ggaattttca tgtcgaaggc
     4561 gcaggccaga tgcagcgcct cgatctcggt c
//
LOCUS       DATNOI010000085         2295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2460853,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000085 DATNOI010000000
VERSION     DATNOI010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2460853"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..739
                     /gene="hslV"
                     /locus_tag="VN968_02965"
     CDS             179..739
                     /gene="hslV"
                     /locus_tag="VN968_02965"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HXL28245.1"
                     /translation="MQTSQSQSPVWHGTTILTVRKGGTVVIGGDGQVSIGQTVIKSNA
                     KKVRKLGKGDVIGGFAGATADAFTLFERLESKLEQYPGQLTRAAVELAKDWRTDRYLR
                     RLEAMMIVADKDVSLVLTGTGDVLEPEAGVMAIGSGGNYALAAARALVDSDKDAEAIV
                     RRSLDIAADICVYTNRNVTIETLQGG"
     gene            743..1228
                     /locus_tag="VN968_02970"
     CDS             743..1228
                     /locus_tag="VN968_02970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL28246.1"
                     /translation="MAPDYLFRPMTLSDLPLIRHWLSFPHVREWWGDPQEQYTLVSGD
                     LDEPAMDQYVFSTAGSPFAYLQCYDLTAWNSGFGPHPPGTRGIDLFIGEPDMIERGHG
                     SALVRWFVDDRLQKGAPRIVTDPDPANGRAVRAYEKAGFEKGRMVESPDGPALLMIRN
                     P"
     gene            1225..1827
                     /locus_tag="VN968_02975"
     CDS             1225..1827
                     /locus_tag="VN968_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 domain-containing protein"
                     /protein_id="HXL28247.1"
                     /translation="MTLERNTTAATGFSIRQWWLFAAGLIALQASILFAMGRLPICAC
                     GYVKLWHGVVQSSENSQHITDWYTFSHILHGFLFYGATWLLLPRLPWQGRLMAAMLVE
                     CSWELVENSSWIIERYRAGTVSLDYFGDSIVNSVSDTLSMVAGFLLARKLPIAATVAL
                     ALLFELVMALHIRDNLTLNVLMLIHPVEAIRLWQGGPPII"
     gene            2047..>2295
                     /locus_tag="VN968_02980"
     CDS             2047..>2295
                     /locus_tag="VN968_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28248.1"
                     /translation="MIASEILAKGVEQGIISAEQAERLRALEDVREPPELPASPDDEQ
                     LRFIGGFSDIFVTIGLAMFLGAIGYFSLQAGGKLGMWIA"
BASE COUNT          412 a          698 c          698 g          487 t
ORIGIN      
        1 agcctatggt gaccggaatc cggcgccggc cttctggttt ggcgcttttt cttcaagtga
       61 accggcgtcc ggccccctgg gatcaagtcc gggggcatgc ctcgttcaaa aacgctccag
      121 accgcgccga ttgcaacccc gaacgccaat ccctaaattc ctgccaacgc gaggtctcat
      181 gcaaacatca caatcccagt cgccggtctg gcacggcacc accatcttga cggtccgcaa
      241 gggcggcacg gtggtgatcg gcggcgacgg ccaggtgtcg atcggccaga ccgtcatcaa
      301 atccaacgcc aaaaaggtcc gcaaactcgg caagggcgac gtcatcggcg ggtttgccgg
      361 cgccaccgcg gatgccttta ccctgtttga gcggctggaa agcaagctcg agcagtatcc
      421 ggggcaactg acccgcgccg cggtcgaact cgccaaggac tggcgtaccg accgctatct
      481 gcgccggctc gaggcgatga tgatcgtcgc cgacaaggat gtgtcgctgg ttctgaccgg
      541 caccggcgac gtgctggagc cggaagccgg cgtgatggcg atcgggtccg gcggcaatta
      601 tgccttggcg gccgcccggg ccttggtcga ttccgacaag gacgccgagg ccatcgtgcg
      661 ccggtcgctg gatatcgccg ccgacatttg tgtgtatacc aatcgcaatg tgaccatcga
      721 gacgttgcag ggcggctgat cgatggcgcc ggattatttg tttcgtccga tgaccctgag
      781 cgatcttccg ctgatccggc actggctctc gttcccgcat gttcgggaat ggtggggcga
      841 cccccaggag cagtatacat tggtcagcgg tgacctcgat gagccggcca tggaccagta
      901 tgtcttttcg accgccggca gcccgttcgc ttacctccag tgttatgatc tgaccgcatg
      961 gaattcaggt ttcgggccgc atccgccagg cacgcgcggc atcgatctgt tcatcggcga
     1021 acccgacatg atcgaacgag gtcacggttc ggcgctggtt cgttggttcg tcgatgatcg
     1081 gctgcaaaaa ggcgccccgc gcatcgtcac ggatcctgac ccagccaatg gtcgcgcggt
     1141 ccgtgcctat gagaaagcag gatttgaaaa gggccgcatg gtagaatcgc ccgatggccc
     1201 cgccctgctg atgatccgca acccatgacg cttgagcgca acacgacagc cgcgaccggt
     1261 ttttcaatcc ggcagtggtg gctgttcgcg gccggcctga tcgccctgca agcctcaatt
     1321 ctgttcgcga tggggcggct gccgatctgc gcctgcggat atgtgaagct ctggcacggc
     1381 gtggtgcaaa gctccgagaa ttcgcagcac attaccgact ggtacacgtt ctcgcacatc
     1441 ctccatggct tcctgtttta tggcgcgacc tggctgctgc tgccgcgcct gccctggcag
     1501 gggcggctga tggccgcgat gctggtcgaa tgcagctggg aactggttga aaattccagc
     1561 tggatcatcg agcgataccg cgccggcacg gtgtcgctgg actactttgg cgacagcatc
     1621 gtcaattcgg tctccgatac gctgtcgatg gtcgcaggct ttttgctcgc ccggaagctt
     1681 ccgattgcgg cgaccgttgc gctcgcactt ttatttgaac tcgtgatggc acttcacatt
     1741 cgcgacaatc ttaccctgaa cgttctgatg ctgatccatc cggttgaggc gatccggcta
     1801 tggcagggtg ggccgccgat catctagagc gggatgactt ttcttcgaat cgtcatcccg
     1861 ctccatcttt ttgtttgagc atgatctccg ggcaaacgct tcgcgtttgt cccgagggaa
     1921 aaccggtatc caccctcgga tcaagtccga gggcatgctt ttccggatca tgctctaggg
     1981 acgaccgcgt cggactgctt gcgagagcgc cgatccggct tagctaatac cagaggttcg
     2041 gtaccgatga ttgcaagtga aattctggcg aaaggggttg agcagggcat catcagtgcc
     2101 gaacaggctg agcgcctgcg tgcactggaa gatgtccgtg agccgcccga attgcccgcc
     2161 tcgcccgacg acgaacagct tcgcttcatc ggcggtttca gcgacatctt cgtcaccatc
     2221 ggccttgcca tgttcctcgg cgctatcggg tatttttcgc tgcaggccgg cggcaagctc
     2281 gggatgtgga tcgcc
//
LOCUS       DATNOI010000086         5693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1395374,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000086 DATNOI010000000
VERSION     DATNOI010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395374"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /gene="argS"
                     /locus_tag="VN968_02985"
     CDS             <1..1713
                     /gene="argS"
                     /locus_tag="VN968_02985"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXL28249.1"
                     /translation="GGWSAGIDLSRVVVEPPRDAAHGDMATNAAMVLAKDAKAKPRDL
                     AEQIATKLRADDLIASVDVAGPGFINLTLKASVWSDALRAVLREGDRYGRSAIGAAEK
                     VNVEYVSANPTGPMHVGHCRGAVFGDALVSLLQFAGYDVTREYYVNDAGAQVDVLARS
                     AFLRYREALGQDIGAIPEGLYPGDYLKPVGQALADEYGDKLLAMSEAEWLPVVRSEAI
                     EMMMEEIKGDLAALHIRHDVFFSERSLIETGNNRVTETIDYLRSKGDVYEGRLPPPKG
                     GAPVEDYEDREQTLFRATAYGDDVDRPLVKSDGSYTYFASDIANHKNKFDRGFSNLID
                     VFGADHGGYIKRMQAAVKAVTAGRATLDVKVVQLVRLLRNGEPVKMSKRSGDFVTLRE
                     VVDEVGSDAVRFMMLFRKNDAVLDFDLAKVIEQSKDNAVFYVQYGHARGHSVFRNARE
                     VVPDLPEEDGARAAFLGVAAVERLTDPAELDLLRRLAAYPRMIEAAAVAHEPHRIAFY
                     LYDLASEFHALWTRGRDLPYLRFIINNDAELTRARLALVQGVVLVLASGLAILGVDAP
                     AEMR"
     gene            complement(1733..2020)
                     /locus_tag="VN968_02990"
     CDS             complement(1733..2020)
                     /locus_tag="VN968_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28250.1"
                     /translation="MLIALGGTRLYLMVGPTHAGEWIVLTDQPREFGQRIAFSLCRRA
                     LTIATIMIVVRWKRSILISISHRDDACPSGKPPTHTYKRTGPCQMPSQVPM"
     gene            2111..3301
                     /locus_tag="VN968_02995"
     CDS             2111..3301
                     /locus_tag="VN968_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXL28251.1"
                     /translation="MEYPEDAPSPVHPLHRYATPRAAAPEPNYRDAAPFADADPEPEP
                     DPSRYDDALYGQADPGTEEPPYDQAYAEDDPYGYQDGYGDDGAEEEPARKRRGGLMTV
                     AIVLALAVVGTGGAFAYRTYVGAPRSGEPPIIRADAGPTKIVPSPANGTSKLPDRMVP
                     GDGSEKIVPREEAPMDVNAKSGPRVVFPSLNQNANPPSPASVASTTLPPASAGNGTLS
                     SNEPRKIRTLSVKGDQPDAAATPVGSTPPPAKPTPATRSVANANASTANAPVALTPQS
                     GSPPAAAPADTRAARVAATNPDQAAPAAGASAPAASASGGYLVQVSSQRNEADAQASF
                     RALQGKFPAVLGSREPVIKRADLGEKGVYYRAMVGPFGSSDEATQFCGSLKSAGGQCV
                     IQRN"
     gene            3358..4386
                     /gene="nagZ"
                     /locus_tag="VN968_03000"
     CDS             3358..4386
                     /gene="nagZ"
                     /locus_tag="VN968_03000"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HXL28252.1"
                     /translation="MSTRAFITGVSGPELTAAEREFIRSERPWGFILFKRNIETPAQV
                     ILLVRELREAIGNPEAPVLIDQEGGRVQRLGPPHWPVYPPGAVFGVLYDIDPALGLTA
                     ARLSARLMAADLTELGVTVDCLPLADVPVAGADAVIGNRAYGTEPGKVAAIARAVTDG
                     LEQGGILPVLKHIPGHGRATADTHFRLPEVDTSKTELERTDFAAFQPLADLPMAMTAH
                     VVFSALDPAQPATTSATIIRRVIRGVIGFQGLLMSDDVSMNALAGSIAERTRAILTAG
                     CDMVLHCNGKLDEMREVAAETPELSGMALERARRALASRAAPQAFDRLAARAELEALV
                     DRARAAGA"
     gene            4383..5213
                     /locus_tag="VN968_03005"
     CDS             4383..5213
                     /locus_tag="VN968_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HXL28253.1"
                     /translation="MSAEILSFETGRPAERLDDEPALVVDVEGYEGPLDLLLALARQQ
                     KVDLAKISILALADQYLVFIEAARKIRLELAADYLVMAAWLAFLKSRLLLPEPVTPDG
                     PSAEEMATALANRLRRLEAIRGASNRLMNRPQLQRDIFPRGDPESIAEIKHPKFTATL
                     FDLLTAYAAQRQQRVLATVHLARRTVWSLNEARASLERLVGMAEDWSRLDEFLLNYVV
                     DPSQKATVFASSFAAALEMVREGEVELHQKEAFAPLYFRKRPPQPVSDAMTAPDLPAE
                     "
     gene            5235..>5693
                     /locus_tag="VN968_03010"
     CDS             5235..>5693
                     /locus_tag="VN968_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HXL28254.1"
                     /translation="MASLAEKRDAEAEEHSVDPEARPEELRLLEALLFASTEPLDQAA
                     LAKRMPEGVDIKVALAQLQADYASRGVNLVRVANKWTFRTAGDLSWLMTRESIETRRL
                     SRAAIEVLAIIAYHQPVTRAEIEDIRGVITSKGTLDVLLETGWIRPRGRRK"
BASE COUNT         1036 a         1773 c         1897 g          987 t
ORIGIN      
        1 ggcggatggt ccgccggcat tgacctgtcg cgggtcgtgg tcgagccccc gcgcgacgct
       61 gcgcatggcg atatggcgac caacgccgcg atggttctgg ccaaggatgc gaaggcaaaa
      121 ccgcgcgatc tcgccgaaca gatcgcgaca aaactgcgcg cggacgattt gattgcgtcg
      181 gtcgatgtcg caggtcccgg cttcatcaat ctcaccttga aggcgtcggt ctggtcggac
      241 gcgctgcgcg ccgtgctgcg cgagggcgat cgctacgggc gaagcgcgat aggcgcggcc
      301 gagaaggtca acgtcgaata cgtctcggcc aacccgactg ggccgatgca tgtcggccat
      361 tgccgcggcg cagtattcgg cgatgcgctg gtaagcctgc ttcagttcgc gggctatgac
      421 gtgacccgcg aatattacgt caatgacgcc ggcgcacagg tcgatgtgct cgcgcgctcg
      481 gctttcctgc gctaccgcga agcgctcggc caggacatcg gcgcaattcc ggaaggactt
      541 tatcccggcg actatttaaa gccggtgggg caggcgctgg cggatgagta cggcgacaag
      601 ctgctcgcga tgtcggaagc cgagtggctg ccggtcgtgc ggagtgaggc tatcgaaatg
      661 atgatggagg agatcaaggg cgatctcgcc gcactccaca tcaggcatga cgtgtttttt
      721 tccgagcggt cgctgatcga aaccggaaac aaccgcgtta ccgaaaccat cgattatctt
      781 cgctccaagg gcgatgttta cgaaggccgc ctgccgcctc caaaaggtgg cgcgccggtg
      841 gaggattacg aggaccgcga gcagacgctg tttcgcgcca ctgcctatgg cgacgacgtg
      901 gatcgtcccc tggtgaagtc cgacggcagc tacacctatt tcgcttccga tatcgccaat
      961 cacaagaaca agttcgaccg cggctttagc aacctcatcg atgtcttcgg cgccgaccac
     1021 ggcggctaca tcaagcgcat gcaggcggcg gtcaaagctg tcacggcggg cagggccacg
     1081 ctggatgtga aagtcgtgca actcgtcagg ctgctgcgca atggcgagcc ggtgaagatg
     1141 tcgaaacgct ccggcgattt cgtcacgctg cgcgaggtcg tcgacgaggt cggctccgat
     1201 gcggtgcggt tcatgatgct gttccgcaag aacgatgcgg tgctggattt cgatcttgcc
     1261 aaggtgatcg agcagtccaa ggacaacgcg gtgttctacg tgcagtacgg gcacgcccgc
     1321 ggccactcgg tgtttcgtaa cgcccgggag gtggttccgg acctgccgga ggaggacggc
     1381 gcccgggcgg cgtttctagg ggtggctgcg gtggagcggc tgaccgaccc tgcagaactc
     1441 gacctgctgc gcaggctggc ggcctacccc cggatgatcg aggcggcggc ggtcgcacat
     1501 gaaccgcacc gaatcgcgtt ttatctgtat gatcttgcca gcgaattcca cgcgctttgg
     1561 accaggggac gcgatctgcc ctatttacgc ttcattatca ataatgatgc agaactcaca
     1621 agggcgcgct tggccttggt ccagggcgtc gttttggtgt tagcatctgg gcttgccatt
     1681 cttggcgtcg acgctcccgc cgagatgcga taaagcaagg ggcgaaggcc gttcacatgg
     1741 ggacctgtga aggcatctgg caggggccag ttcgtttgta agtgtgggtt ggcggctttc
     1801 ccgaagggca cgcatcatca cgatggctga tcgatatcag gatagaccgt ttccagcgga
     1861 cgacgatcat gatcgtcgcg atggtcagcg cgcgcctgca caggctgaaa gcgatccgct
     1921 ggccgaactc gcgcggctga tcggtcagaa cgatccattc gccagcatgg gtcgggccaa
     1981 ccatcaggta cagccgcgtg ccgccgagcg cgatcaacat cgctcacccg cggccccggc
     2041 cccggatgat gccccggtcg cgagcccgcc gccgtggatg cagcgcgcta ctcggcagga
     2101 actcccgcag gtggagtatc cagaagatgc cccgagcccg gtccatccgc tgcatcgcta
     2161 cgcaaccccg cgtgcagcag cacccgagcc gaattatcgc gacgcggcgc cgttcgcgga
     2221 cgccgatccc gagcccgagc ccgatccatc gcgctatgac gacgcgctgt acggtcaggc
     2281 cgaccccggc acggaggagc ctccatacga tcaggcctat gcggaggatg acccctacgg
     2341 ttaccaggac ggctacggcg atgacggtgc cgaagaggag ccggcccgca agcgtcgcgg
     2401 cggcctgatg accgttgcta tcgttctcgc gctggcagtg gtgggaactg gcggagcgtt
     2461 tgcctatcgt acctatgtcg gagctccccg cagcggcgag ccgccgatca tcagggccga
     2521 cgcaggcccg accaagatcg tgccgtcgcc cgccaacggg acgtccaagc tgccggatcg
     2581 catggtgcca ggcgatggca gcgagaaaat cgtgccgcgc gaagaagcgc cgatggacgt
     2641 caacgccaaa tctggcccgc gcgtggtgtt tccgtcgctg aaccagaatg cgaatccgcc
     2701 cagcccggca agcgtcgctt cgaccacgct gccgccggcg agcgccggga atggcacgct
     2761 gtcgagcaat gagccacgaa agatcaggac cctgtcagtc aagggcgacc agcccgatgc
     2821 agccgcgacg ccggtcgggt cgacgccgcc ccccgcaaag ccgacgccgg ccacccgttc
     2881 ggtcgccaac gcaaatgcct cgaccgccaa tgcaccggtg gcgttgacgc cgcagagcgg
     2941 ctctccgccg gccgccgccc cagcggatac gcgggcggcg cgggttgctg ccaccaaccc
     3001 cgatcaggcc gcgcccgctg ccggcgcaag cgcgcccgct gccagcgcaa gcggtggata
     3061 tctggttcag gtctcctcgc aacggaatga ggccgatgcg caggcttcat ttcgggccct
     3121 gcagggcaag ttcccggcgg tgctgggatc tcgggaaccg gtgatcaagc gcgccgacct
     3181 cggcgaaaag ggcgtctatt accgcgccat ggtcggccca ttcggatcat cggatgaagc
     3241 gacacagttc tgcggcagcc tgaaatccgc cggcggacag tgcgtcatcc aaaggaatta
     3301 acggccgttt ccctgaccct ggttgaccgc tgggccgcgt gagggctaat cggccctatg
     3361 agcacgcgcg cattcatcac tggcgtatcc ggaccggagc ttaccgccgc tgaacgtgaa
     3421 ttcatccgtt cggagcggcc gtggggcttt attctattca agcggaatat cgagacgccg
     3481 gcgcaagtaa ttctgctggt tcgcgaactg cgcgaggcga ttggcaatcc cgaggcgccg
     3541 gttctgatcg accaggaggg aggcagagtc cagcgcttgg gcccgccgca ctggccggtt
     3601 tacccacccg gagctgtctt tggggtgctt tatgacatcg accctgcgct cggcctgacc
     3661 gcggcgcgct tgagcgcgcg gctgatggcg gcggacctta ccgagctcgg cgtcaccgtc
     3721 gattgcctgc cgctggcgga tgtgccggtc gccggcgccg acgcggtgat cggcaaccgg
     3781 gcctatggaa ccgagccggg caaggtggcg gcaatcgcgc gcgccgtgac ggacggtctg
     3841 gagcagggcg gcatattgcc ggtgctcaaa catattcccg ggcatgggcg ggccaccgcc
     3901 gacactcatt tccggcttcc ggaggtcgat acatcgaaaa cggagctgga acggaccgat
     3961 ttcgccgcgt ttcaaccgct tgcggacctg ccgatggcga tgaccgcaca tgttgtgttt
     4021 agcgcgttag atcctgccca acctgcgacg acttctgcga caatcatccg acgggtgatt
     4081 cgcggcgtga tcgggttcca gggtttgttg atgagtgatg atgtgtccat gaacgcgctg
     4141 gcaggatcga tcgccgagcg gacccgggcc atcctcaccg ccggctgcga catggttctg
     4201 cattgcaacg gcaagctcga cgaaatgcgc gaggtcgcgg ccgagacgcc cgaactgtct
     4261 ggcatggcgc tggagcgcgc cagacgggca ctggcgtcac gtgctgcacc gcaagcgttc
     4321 gatcggctag cggcgcgggc tgaattggaa gcattggttg atcgggcaag ggcggcaggc
     4381 gcatgagcgc ggaaattcta tcgtttgaaa ccggacggcc cgcagaacgc ctggacgacg
     4441 agccggcgct ggtcgttgac gtcgaaggct atgagggccc gctcgacctg ctgctggcgc
     4501 tggcgcggca acagaaggtc gatctcgcca agatttccat cctggcgctg gccgaccagt
     4561 atctggtgtt catcgaggcg gcccggaaga tccgcctcga gcttgccgcc gactatctcg
     4621 tcatggcggc atggctcgcg tttctgaaat cgcggctgct gctgcctgaa ccggtaaccc
     4681 cggacggacc gagcgccgag gagatggcga cagcactggc caaccggctg cgccggcttg
     4741 aggccattcg cggggcatcg aaccggctga tgaaccgccc gcaattgcag cgcgacattt
     4801 ttccgcgcgg cgatccggaa tcgatcgccg agatcaagca tcccaagttt accgcaaccc
     4861 tgttcgacct gctcaccgcc tacgccgcgc agcgccagca gcgggtgctg gcaacggtgc
     4921 atctggcaag acgcacggtg tggtcgctga acgaggcgag ggcatcgctg gagcgccttg
     4981 tcgggatggc cgaggactgg agccgcctcg acgaattcct gctcaactac gtcgtcgatc
     5041 cctcgcaaaa ggcgaccgtt tttgcgtcca gctttgctgc ggcgctggaa atggtcaggg
     5101 agggcgaggt cgagttgcac cagaaagagg cgttcgcgcc gctctatttt cgaaagcgtc
     5161 cgccgcagcc agtgtccgac gcgatgaccg cgccggatct gccggccgag taagtggaag
     5221 aaggagaccg tgccatggca agcctggccg aaaaacgcga cgctgaagcc gaggagcatt
     5281 ccgtggatcc agaagcgcgg cccgaggaat tgcggctgct ggaagcgctg ctgttcgcct
     5341 cgaccgagcc gctcgatcag gcggcgcttg ccaagcggat gcccgaaggc gtcgacatca
     5401 aggttgcgct ggcgcagctg caggcggatt acgcctcgcg cggcgtcaat ctggttcgcg
     5461 ttgccaataa atggaccttc cgcaccgcgg gcgatctgtc ctggcttatg acgcgggaga
     5521 gcatcgagac gcgccggctg tcgcgcgcgg cgatcgaggt gctcgcgatc attgcctatc
     5581 atcagccggt cacgcgcgcg gagatcgaag acatccgcgg cgtgattact tccaaaggga
     5641 cgctggatgt cttgctggaa accgggtgga ttcgcccgcg cggccgtcgc aag
//
LOCUS       DATNOI010000087         3882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1181229,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000087 DATNOI010000000
VERSION     DATNOI010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181229"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /locus_tag="VN968_03015"
     CDS             <1..1498
                     /locus_tag="VN968_03015"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HXL28255.1"
                     /translation="RGLLVVSGAQGSKLDPSSEEGISAKMGIDATKPVEAEVMKFKRI
                     HVKGLEEVDLDSVLQQDAKAAFASLAGSSDGTLARSGSCAIALLLARHNTVSDAILIL
                     NAGSSSIKFSVFPGHQRPSRQSIICEGECEGIGHRVHFTAKDRAGVSLISEYLTEGTT
                     HEGALAALLRWLEGRFLDNQLVAAGHRVVHGGSLYAAPVLVDASVTAELCRLIPLAPL
                     HQPHNLAAIAALTKLHPGLPQIACFDTAFHHTQTEVATAFALPRRLAAEGIRRYGFHG
                     LSYEYIASVLPDILGPAAADGRVVVAHLGSGASLCAIHQRKSVATTMSFTALDGLPMS
                     RRCGNVDPGVILYLMQEKAMTAAEVSNLLYNESGLFGVSGVSDDMRTLLASDDPSAKQ
                     AVALFVYRIGRELGSMVAALGGIDALVFTAGIGEHGAEIRRRVCEDAAWLGVDPDQGA
                     NAAGRAMITRAGSRTSAWVIPTDEDLMIALHAWTLLNPEAASPQRWPG"
     gene            1565..2443
                     /locus_tag="VN968_03020"
     CDS             1565..2443
                     /locus_tag="VN968_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXL28256.1"
                     /translation="MIQSEDNKISEDRREALRYALAIGSGAALAAAVAGPASAQATPD
                     NTLDRIRANKVLRIAVLPGELPYFNKDLATGNWSGFSIEMANDIAKLLDVKLEYTEST
                     YGNSILDLQSNKIDLGFALNPTPQRALVVDFCATVFPHPFGAMLKKGLEARTWADINK
                     PDVNIAVDVGSANEAVARRFAPNATIKSLKSRDEVMLEMSSGRVDCVVNALVLGLTAI
                     AKNPNLGTYRILQSPAVAIPSSMAVRREPDKRWRDFLSVWVDYNRGIGQMREWFTKGL
                     SLASVKPEDVPVELNI"
     gene            2569..3063
                     /locus_tag="VN968_03025"
     CDS             2569..3063
                     /locus_tag="VN968_03025"
                     /inference="COORDINATES: protein motif:HMM:NF023982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL28257.1"
                     /translation="MRRGAEVDVELVATAIHDVRELIGELDRVLAAEYLPEQRHGLVL
                     EALIQPHIRFFVARLNGAAIGCGGVALFDDFAEVKRMYVRETVRWRGVAQALLTRIET
                     EARVAGLVVLRLETGERQAAALRFYAQAGFKPCAAFGDYAQMRPEAIATSIFMEKRLS
                     PPAA"
     gene            complement(3149..3817)
                     /locus_tag="VN968_03030"
     CDS             complement(3149..3817)
                     /locus_tag="VN968_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXL28258.1"
                     /translation="MPYVWDFAILGKYSHLFWLGLGWTIAYTIGTVLLGTVLGLVVGI
                     LRLRRWPVIDWLLLAYVELFRCTPLLVQIIWFYYALPVVIGVNIPAHAAAVSVLSLYA
                     GAFYAEIVRGSIESVHRGQWDAARALGLQPWRVLRYVILPQALKPMLAPYVNQSVTQL
                     KNTSLVSIIAVPDLVYNATLINAETYRPLEVYTIVALIYFAILFPATLVARRFERGLT
                     YDKA"
BASE COUNT          731 a         1173 c         1255 g          723 t
ORIGIN      
        1 ccgcggcctg cttgtggtct ccggtgcgca gggctcgaaa ctcgatccgt caagcgagga
       61 gggcataagc gcaaagatgg ggatcgacgc gacgaagccg gtggaagctg aagtgatgaa
      121 attcaagcgc atccatgtga agggtttgga agaagtcgac ctggatagcg tgctgcagca
      181 ggacgcaaag gccgcgtttg cgagcctcgc tgggagctcg gacgggacgt tggcaagatc
      241 gggatcctgc gcgatcgcgc tgctgctggc ccgtcacaac accgtgagtg acgctatcct
      301 gatactgaat gccggttcgt cgagcatcaa gttttcggtg ttcccgggac atcagaggcc
      361 gagccggcag agcatcattt gcgaaggtga gtgcgagggc atcggccacc gcgttcattt
      421 cacggcaaaa gaccgcgccg gagtgtcgct gatctctgag tatctgacag agggcactac
      481 gcacgaaggc gcgttggccg cactgctgcg ctggctggaa ggccgctttc ttgacaatca
      541 actggtggcc gccggacatc gggtggtgca cggcgggtcg ctttatgccg cgccggtcct
      601 tgtcgacgcg tcggtgaccg ccgagttgtg ccgcctcatt ccgctggccc cgctccatca
      661 accccacaac ctcgccgcca tcgccgccct gacaaagctt catcctggat tgccgcagat
      721 cgcctgcttc gatacggcct ttcatcatac gcagaccgag gtcgccactg cattcgcgct
      781 gccgcggcgg ctggccgccg agggcatccg gcggtacggg tttcatggcc tttcctatga
      841 atacatcgca agcgttctgc cagacatcct tggtccggca gctgccgacg gccgcgttgt
      901 cgtggcccat ctcggcagcg gcgctagcct gtgtgcgatc catcagcgca agagcgtcgc
      961 gacgaccatg agctttacgg cgctcgatgg cctgcccatg agccgccggt gcggcaatgt
     1021 cgatccgggc gtcatcctct atttgatgca ggagaaggcg atgaccgcgg ccgaggtgag
     1081 caatctgctt tataacgagt ctggactgtt tggtgtttcc ggcgtcagcg acgacatgcg
     1141 gaccctgttg gccagcgacg atccgtcggc gaagcaggcg gtcgctctgt tcgtgtatcg
     1201 gatcggccgc gaactgggct ccatggtcgc cgccctcggc gggatcgatg ctttggtctt
     1261 caccgcggga atcggggagc acggggctga aattcggcgc cgggtttgcg aggacgccgc
     1321 atggctcggc gtggatccgg atcaaggcgc aaatgcagcc ggccgagcca tgatcacgcg
     1381 cgccggcagc cgtacctcgg cctgggtgat tccgacggac gaagatctga tgatcgccct
     1441 gcatgcgtgg acgttgttga accccgaggc ggcgtcacct cagcgatggc cgggataacc
     1501 ctcgtgctat tgctgcgtcg caacgcgcgc gctaggctct gccgaattgg tgcaggaggc
     1561 ccccgtgatc cagtccgagg acaacaagat ttccgaagat cgccgtgaag cgctgcgcta
     1621 cgcccttgcc atcggcagcg gcgcagcgct tgctgccgct gttgcaggcc cggcgtcggc
     1681 acaagcaacc ccggacaaca cgctcgatcg catccgcgcc aacaaagtgc tccgcatcgc
     1741 ggtactgccg ggagagctgc cttacttcaa caaggacctt gctaccggca actggtccgg
     1801 cttctcgatc gagatggcca acgatatcgc gaagctcctc gacgtcaaac ttgaatatac
     1861 cgagtcgacg tacggaaatt cgatcctcga cctgcaatcg aacaagatcg acctcggctt
     1921 tgccctcaat ccgaccccac agcgggcgct tgtcgtggat ttttgcgcaa ccgtatttcc
     1981 gcatccgttt ggcgccatgc tcaagaaggg gctggaagcc agaacctggg ccgacatcaa
     2041 caagcctgat gtcaacatcg cagtcgacgt cggctccgcc aacgaggccg ttgcgcggcg
     2101 cttcgcgccc aacgcgacga tcaagtcgct caagtcacgc gatgaggtca tgctcgagat
     2161 gtcgtcgggc cgggtcgatt gcgtggtgaa tgcgttggtg cttggtctga ccgccattgc
     2221 caagaaccca aaccttggca cttacaggat cctgcaatcc cccgcagtgg ccataccaag
     2281 cagcatggcg gtccggcgcg agccggacaa gcgctggcgc gatttcctgt cggtgtgggt
     2341 cgactacaac cgcggcatcg ggcagatgcg cgaatggttc accaaggggc tgtcgctcgc
     2401 gagcgtgaag ccggaagacg tgccggtcga acttaatatc tgacgcggtt ttcgaaccgg
     2461 tcaagccttg tcgcaggtca ggccacgctc gaacctccgc gcgacaagcg tggccgggaa
     2521 caggatcacc gatcgcatgc acctccagcg gccgaatcgg gagacgaggt gcgcaggggc
     2581 gcagaagtcg acgttgaatt ggtagcgacg gcaatccacg acgtgcgcga gctgatcggc
     2641 gaactcgacc gggtgcttgc ggccgaatac cttcccgagc agcggcacgg gctggtgttg
     2701 gaggcgctca tccaaccgca cattcgcttc ttcgtggcgc ggctgaacgg cgccgccatc
     2761 ggctgcggtg gggtggccct gtttgatgat ttcgccgaag tcaagcgcat gtatgtgcgc
     2821 gagacagtac gttggcgtgg cgtggcgcag gccttgttga cgcggatcga aacggaggct
     2881 cgcgtcgctg gacttgtcgt cttgcgcctg gaaacgggag aacggcaggc ggcggcactg
     2941 cggttttatg cccaggccgg cttcaagcct tgcgcggcat tcggtgacta cgcgcagatg
     3001 cggccagaag ccatcgcgac cagcatcttc atggaaaagc ggttgagccc acctgccgcc
     3061 tgatgtcggc gctgacgcgt gccgcggccc ggtcggtggc gtggtggttt ctcagacggc
     3121 tctgcatctg acgcggtgtt cgaaccggtc aagccttgtc gtaggtcagg ccacgctcga
     3181 acctccgcgc gacaagcgtg gccgggaaca ggatcgcaaa gtagatcagg gccacgatcg
     3241 tatatacctc cagcggccga taggtctcgg cgttgatcag cgtcgcgttg tagacgaggt
     3301 ccggaacggc aatgatggac acaagcgagg tgttcttcag ctgtgttacc gactggttga
     3361 cgtagggcgc caacatcggc ttgagcgcct gcggcaggat cacgtatctc agcacgcgcc
     3421 atggttgcag tcccaaggcc cgcgccgcgt cccattgtcc gcggtgcacg ctttcgatgc
     3481 tgccacggac gatttcagca taaaatgctc cagcatagag cgagagcacg ctgacggcgg
     3541 cggcatgtgc cggtatgttc accccgatga cgaccggcaa ggcgtagtag aaccagatga
     3601 tctgaaccaa caggggtgtg cagcggaaca gttcgacgta ggcgagcaga agccaatcga
     3661 tcacgggcca gcgcctcagg cgaaggatgc cgacgaccaa tccgagcact gttccgagca
     3721 ggacggtacc gatcgtgtag gcgatggtcc agccgagccc gagccagaac aggtggctgt
     3781 atttgccgag gatcgcgaaa tcccaaacgt agggcatgtc tgctgccgcc tactgcgacc
     3841 ctcttgtttg ccgtctgtcg gccatcagag gtagcggccc gg
//
LOCUS       DATNOI010000088         3266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1180831,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000088 DATNOI010000000
VERSION     DATNOI010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180831"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VN968_03035"
     CDS             <1..1077
                     /locus_tag="VN968_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HXL28259.1"
                     /translation="VLPNGDVLVAETNAPPKPDDTSGIKGWITKMVEGRAGAGTPSAN
                     RITLLRDADGDGVAETKTAFLEGLNSPFGMALVGDDFYVADSDAIMKFPHHADDTKIT
                     APGVKLTDLPAGTINHHWTKDLTASPDGTKLYATVGSNSNVGENGIEAETNRAAVLEV
                     DRATGKWRVFASGLRNPNGPSWQPQSGALWVAVNERDELGNDLVPDYMTSVKDGGFYG
                     WPYSYFGQHVDDRVSPQRPDLVAKAIAPDYALGAHTASLGLTFNTGNLFPSAMTGGAF
                     VGQHGSWNRTPRAGYKVIFVSFANGKPSGAPQDVLTGFLNDKGEAQGRPVGVKIDRQG
                     ALLVADDVGNAVWRVTPAEKSAAR"
     gene            complement(1197..2303)
                     /locus_tag="VN968_03040"
     CDS             complement(1197..2303)
                     /locus_tag="VN968_03040"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HXL28260.1"
                     /translation="MPFTLPPDANGILPDRMIAAMADAGLILPAYPFVESQIQPASLD
                     LRLGDVAYRVRASFLPGPGATVAERIDELKLHEINLSDGAVLETNCVYIVPLLESLAL
                     PPQIVAAANPKSSTGRLDVFTRVIADGTRRFDMIGAGYHGPLYAEISPKTFPVLLREG
                     SRLSQVRFRTGDAILNADELDALHDLERLVDCDDADLLNGVALSVDLSGENADGFVGY
                     RAKRHTGVVDIDRRGGYAVDEFWEAISARPDGSLILDPGEFYILASKEAVQVPPDYAA
                     EMMPFDPLVGEFRVHYAGFFDPGFGYAGAGGKGSRAVLEVRSREVPFILEHGQIVGRL
                     VYEKMLARPDALYGQRIGSNYQAQGLKLSKHFRV"
     regulatory      2415..2494
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            2562..>3266
                     /locus_tag="VN968_03045"
     CDS             2562..>3266
                     /locus_tag="VN968_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXL28261.1"
                     /translation="MSDATLPVSSTAHYRPETRLVHSGALRSQFGETSEALFLTQGFV
                     YDSAEQCEARFKGDDPGFLYSRFSNPNVSMFERRMIELEGAEAARATATGMAAVTTAV
                     LAPLKAGDHVVAAKAMFGSCRYVVEDLLPRYGIGSTLVDGLDLDQWRRAMRPNTKSCF
                     LESPTNPTLDVLDIGAIADIAHRAGARLIVDNVFATPIWQSPLELGADVVVYSATKHI
                     DGQGRCLGGIILSSEAF"
BASE COUNT          612 a         1052 c         1038 g          564 t
ORIGIN      
        1 gtcctgccca acggcgacgt gctggtggct gagaccaacg ccccgccaaa acccgacgat
       61 accagcggca tcaaaggctg gatcacgaaa atggtcgagg gccgcgccgg cgccgggaca
      121 ccgagcgcga accgcatcac gctgctgcgg gacgccgatg gcgatggcgt cgccgagacc
      181 aagacggcct ttctggaagg cctgaattcg ccgttcggca tggcgctggt cggcgacgat
      241 ttttatgtcg ccgattccga cgcgatcatg aaatttcccc accatgccga cgataccaaa
      301 atcacggcgc ccggcgtgaa gctgacggac ctgccggcgg gcaccatcaa tcaccactgg
      361 accaaggatc tcaccgccag tcccgacggc accaaacttt atgccaccgt cggctccaac
      421 agcaatgtcg gcgagaacgg catcgaggcc gaaaccaacc gcgccgccgt gctcgaggtc
      481 gaccgtgcga ccgggaagtg gcgcgtgttc gcctcgggcc tgcgcaatcc gaacgggccg
      541 tcgtggcagc cgcagagcgg cgcgctgtgg gtcgcggtca atgagcgcga cgaactcggc
      601 aacgacctcg tgccggacta catgacctcg gtgaaggacg gcggcttcta tggctggccc
      661 tacagttatt tcggtcagca tgtcgacgac cgcgtcagcc cgcagcggcc ggatctggtg
      721 gcgaaggcga ttgcgccgga ttacgcgctg ggcgcccaca ccgcttcgct cggactgacc
      781 ttcaacaccg gcaatctgtt tccatccgcg atgacgggtg gtgcattcgt cggccagcac
      841 ggctcctgga accgaacacc ccgcgccggc tacaaggtga tctttgtgtc gttcgccaac
      901 ggcaagcctt cgggcgcgcc gcaggacgtg ctgaccggct tcctcaacga caagggcgaa
      961 gcccaggggc gcccggtcgg cgtcaagatc gacaggcagg gcgcgctcct ggtggccgac
     1021 gatgtcggca acgcggtgtg gcgcgtgacg ccggcggaga agtcggcggc gcgatgaggc
     1081 accatcgtca ttgcgagcca acggccggcg caaagcgccg cccgatgaca ggctccgcga
     1141 agcaattcag cgagaggact ggattgcttc gtcgctaacg ttcctcgcaa tgacggctaa
     1201 acccgaaaat gcttggagag cttcaggccc tgcgcctggt agttcgagcc gatgcgctgg
     1261 ccgtacaggg cgtccggccg ggcgagcatt ttctcgtaga ccagccggcc gacgatctgg
     1321 ccgtgttcga ggatgaacgg cacctcgcgc gagcgcactt ccaataccgc gcgcgagccc
     1381 ttcccgccgg caccggcata gccgaaacct ggatcgaaga agcccgcata atgcacgcgg
     1441 aattcgccga ccagggggtc gaacggcatc atctccgcgg cgtagtccgg cggaacctgt
     1501 actgcttcct tcgaggctag aatgtagaac tcgccgggat cgagaatcag gctgccgtcg
     1561 gggcgcgcgg atatcgcttc ccagaattca tcgaccgcat agccgccgcg gcggtcgata
     1621 tcgaccacgc cggtgtgacg cttggcgcgg taaccgacga agccatcggc gttttcgccg
     1681 ctcagatcga cgctgagcgc cacgccgttg agaagatcgg cgtcgtcgca atcgaccagc
     1741 cgctccagat catgcagcgc gtcgagctca tcggcattca ggatggcgtc cccggtgcga
     1801 aaccgcacct ggctgagccg cgatccctcg cgcagcaaca ccggaaacgt tttcgggctg
     1861 atctcggcat agagcgggcc gtggtagccg gcgccgatca tgtcgaaccg ccgcgtcccg
     1921 tccgcgatca cccgggtgaa cacgtcgagc cgtccggtcg agcttttcgg attggcggcg
     1981 gcaacgattt gcggcggcag cgccagactc tccagcagcg gcacgatgta gacgcagttg
     2041 gtctccagca ccgcgccatc gctcagattg atttcatgca gcttcaattc gtcgatgcgc
     2101 tcggccaccg tagccccggg ccccggcagg aaactcgcgc gcacccgata ggcgacgtcg
     2161 ccgagccgca gatcgaggct cgccggctgg atctggctct cgacaaaggg ataggcaggc
     2221 agaataaggc cggcatcggc catcgccgcg atcatgcggt cgggcaagat gccattggcg
     2281 tcgggtggaa gtgtgaacgg cacgtctgat tgtccttgcc aacgcgtccc tgccatccag
     2341 acgctgaaaa tacggacttt tatgcgttat ccgatggacg ccttgacgga aagggcgtga
     2401 acgaatatga gcatgactta tcccgtggtg atttgagccg gccggcttgc agccacgtta
     2461 aataagtcgc taaacaggcc ggggacatat gtgatcccgg cctgtggaat ttttccaggc
     2521 cggttttttg tgaccattcc catggtcacg atggaggtca catgtctgat gctacgttac
     2581 cggtttcgtc aaccgcccat taccgtcccg aaacccggct ggtgcattcc ggcgccttgc
     2641 gctcgcaatt cggcgaaaca tccgaggcgc tgttcctgac ccagggcttt gtctacgaca
     2701 gcgccgaaca atgcgaggcg cggttcaagg gcgacgatcc cggctttctt tattcgcgct
     2761 tttccaatcc caacgtgtcg atgttcgagc gccgcatgat cgagctcgag ggcgcggaag
     2821 cggcgcgcgc caccgccacc ggcatggcgg cggtgaccac ggccgtgctg gcgccgctga
     2881 aagccggcga tcatgtggtg gccgcgaaag cgatgttcgg ctcgtgccgc tacgtggtgg
     2941 aggatctgct gccgcgctac ggcatcgggt ccacgttggt cgacggcctc gatctcgacc
     3001 agtggagacg ggcgatgcga ccgaacacca agagctgctt tctggaaagc ccgaccaatc
     3061 cgacgctcga cgtgctcgac atcggcgcga ttgccgacat cgcccatcga gccggcgcac
     3121 ggctgatcgt cgacaacgtg ttcgcgacgc cgatctggca gagcccgctt gaattgggcg
     3181 ccgacgtcgt ggtgtattcc gcgaccaagc atatcgacgg ccagggccgt tgcctcggcg
     3241 gcatcatcct ctcctcggaa gcgttc
//
LOCUS       DATNOI010000089         6844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_669878,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000089 DATNOI010000000
VERSION     DATNOI010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6844
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669878"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VN968_03050"
     CDS             complement(<1..303)
                     /locus_tag="VN968_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HXL28262.1"
                     /translation="MLALTFKHILADFVLQTSWMAIGKDQKTGWALPLLAHCLVHLAV
                     AMTLILIVAPRFWFVALIDFAIHITVDRAKGYCSSRFCVTQENHHPWFWTLIGVDQA"
     gene            723..2123
                     /locus_tag="VN968_03055"
     CDS             723..2123
                     /locus_tag="VN968_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXL28263.1"
                     /translation="MSAELPTPDAQTPPAQSRVVRSNRRQVLLFAALTLILTVATVWG
                     GRTWVRNSETLIFAVGDANSLDARFAAKLAAVLKNSSSRLRLKIVANADSAKALAQFD
                     RRQADLAVLRTDAKVPPRARALAILEHDVLLLISPGGKKIKSLAELKKKKIAVLADAD
                     NSVAFVRTILEISDNADGGPRVQMAPPNSTIERLFASGGYGAAIAIAHASKIVKDKSY
                     EQSARHGGFTLNAIDEAKALARKNPGISEETLTAGMLSSAPAIPDDDLDTIGLQWLLV
                     AQSRMSSTTAGDLARTIYENKAELALADGFASRIEPADTDKDAFIVAHQGAAEYINDD
                     TKSFMDRYSDLLYLGAAALSVIGSIFAGIYTKFTRVAPEKAGELATAILDIGERVEHA
                     DSLDQLEALQDELEAILRGAVIGLRDGTISSNGLDTFKLGYEFVRDGIGMRRDHLKRN
                     AGHDGNVVVVKSAQSA"
     gene            complement(2194..3051)
                     /locus_tag="VN968_03060"
     CDS             complement(2194..3051)
                     /locus_tag="VN968_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HXL28264.1"
                     /translation="MAGVLSSLRYLLADVIGDDDEAPPFLPNGLPEGAAPIVSEGIHL
                     LIDYQGPSYAGLYVQRLRRFVGRRGVDDVMFGEIARLMAMRMSYEDPIRIAQSKLAET
                     EMASGAPNVQFADDVRKFRLDELIGALPAVVAEPALDALEWVGWLHMPVSIRFGNASR
                     WGIRRLRIEAGLRRWRLFSVRYAKERVWVERWLHMIDRSLTKQPAAASTIVQTATMVR
                     GYGDAYRQALADWHAIIDGLVKPTLDENLPLADLAAAVAEARAAVTPDPRQAALKRTI
                     AEIRARARG"
     gene            complement(3069..3731)
                     /locus_tag="VN968_03065"
     CDS             complement(3069..3731)
                     /locus_tag="VN968_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXL28265.1"
                     /translation="MDTSHFAEHAGSAASLFASVFVVATLSMRTMIPLRVFGILTNVV
                     LIASSIPSHNYPTGALQTVLLVLNSYRLHQMLQLVREVKKSVNSDLSMEWLKPFMAER
                     QCAAGEVLFYKDEKAEEMLYIVSGRFKLVESGIELPVGAIVGELGMLSPSNMRTQTLE
                     CIEAGLILSVSYTKVEELYVQNPAFGFYFLRLASARLFQNIATLEQRLAHHAAAAAPN
                     PA"
     gene            4033..5145
                     /locus_tag="VN968_03070"
     CDS             4033..5145
                     /locus_tag="VN968_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-gated ion channel"
                     /protein_id="HXL28266.1"
                     /translation="MFKPLAFPGLAEFAAATAGRNMTKAAYVAVAAGVAMMVLLTVDP
                     AYETAHHWVDGLLWGCLAFFAFEWIVRLRHAVLTQRGWAYALSGRGLVDAVGALAVPL
                     ALVLGANPKTAWLLGIFWMFKVVPGIPGLRQLRRVLVQESGPLFSVLVIFLMVLFLAS
                     VSVYFLERDVQPGTFGSVPAALWWAIATLTTVGYGDVVPITPLGRLVAAVVMICGLGV
                     FGLWTGILATGFAAETRRDNFLKTWESVSKVPFFAALGPAAIADVTHMLRTMDLPART
                     MIIRKGQTGDCMYFIAAGEVEVDLPGKKVTLGEGAFFGEMALLGNNLRSANITTKRVS
                     RLLVLDLVDFRMLMARHPDLAETIDVEARRRELENK"
     gene            5157..5942
                     /locus_tag="VN968_03075"
     CDS             5157..5942
                     /locus_tag="VN968_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXL28267.1"
                     /translation="MPEATATASAPLLDIDGARATIRLNRPRHLNRLQADDLDALLKL
                     FDRIEADPAVRVMVLTGTGRAFSAGYDLNSVAERATSAIEQKSAGSAFEAVVNRLEDL
                     GVPSICRINGGVYGGATDLALACDFRIGVDTCEMFMPAARLGLHYYKSGIRRYVSRLG
                     ADNARKLFLTAQKIGATEMLRIGYLTAMAPPEALDEEVDKLATILAGNAPVAMRGMKR
                     TINEIARGALDEDAADQRHRDSMRGAEIREGIKAFAEKRPPRF"
     gene            complement(5984..6706)
                     /locus_tag="VN968_03080"
     CDS             complement(5984..6706)
                     /locus_tag="VN968_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HXL28268.1"
                     /translation="MSDTALKTSASRHSVEQRIGVLEELLSERYSCRAFRPDPVPRLT
                     IERILTAAQKTASWCNSQPWQLAIASGAATQKFRDAIHGAASSGKPNTGDFPFPREYR
                     GVYLERRRESGFQLYNSLGIPRGDKAGYAKQALENFNFFGAPHVAIVHTDEALGVYGA
                     IDCGGYVTSFMLAAQALGVATIPQAALAFHSELVRKHFGLGDDRRVVCGISFGFPDRD
                     HKANSYRTTRASVADTVTFFEE"
     gene            complement(6709..>6844)
                     /locus_tag="VN968_03085"
     CDS             complement(6709..>6844)
                     /locus_tag="VN968_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXL28269.1"
                     /translation="PSMRIAGGTDEILRNIIAERVLGLPGDIRVDKNVPFNKIPTKGR
                     "
BASE COUNT         1303 a         2127 c         2135 g         1279 t
ORIGIN      
        1 tgcctggtct accccgatca gcgtccagaa ccatggatga tgattctcct gcgtcacgca
       61 aaacctcgat gagcaatatc ccttggcgcg gtcgaccgtg atgtgaatcg caaagtcgat
      121 cagggcaaca aaccagaatc gcggcgcgac aatcaggatc agcgtcattg caaccgcaag
      181 atgcaccaga cagtgggcca gcagcggcaa cgcccagccg gtcttttggt ccttgccaat
      241 cgccatccag gaggtttgca gaacgaaatc cgccagaatg tgtttgaacg tgagggccag
      301 catccaccct atcagcgcac ctgccggaac cgacgaagac atggggggaa acaacaaagc
      361 tgacgccctt tgctgggact gactggaatg acattaagaa ctgtaactca ggctgtcagc
      421 gcaacttctc ttcaatcccg atcaattcaa gtgcgatatt acttatttat gacacctccc
      481 gggctggaat gcaccagcgg ggaaccgaaa aacgctaggt gtgacctaac agtaatatgc
      541 acagcaacat caatatgcgg tccaccggta acgttacccc gcagccttat ttgcaatcca
      601 ctcctgggca gatatcatcc ttaactggaa aattgtcgtt cttttttaga tatttacgat
      661 cttgttcggg cgatcgtcgg cgccgtctgg cggcgaacca ccctgggttg cggttatgaa
      721 cgatgagcgc tgaactcccg acgccagatg cgcaaacgcc gcccgcgcag tcgcgggttg
      781 tcagaagcaa ccgcaggcag gtgctgctgt ttgccgcgct gacattgatc ctcactgtcg
      841 cgacggtttg gggcggtcgc acctgggtga ggaattccga aacgctgatc ttcgccgtcg
      901 gagacgccaa cagccttgac gcccgttttg ccgccaagct tgccgcggtg ctcaagaaca
      961 gttcttcgcg gctgcgcctc aagatcgtgg ccaacgccga cagcgccaag gcgctcgctc
     1021 agttcgatcg caggcaggcc gatcttgcgg tgctgcgcac cgatgcaaag gtcccgccgc
     1081 gcgcccgtgc gcttgccatc ctggagcacg acgtgctgct gctgatcagc cctggcggca
     1141 agaagatcaa atcgcttgcc gagctgaaga aaaagaagat cgcggttttg gccgatgccg
     1201 acaacagcgt cgcgttcgtc cgcaccatcc tcgagatttc cgataacgcg gatggcggtc
     1261 cacgggtgca gatggcgccg ccgaattcga caatcgaaag gctgttcgcg tccggtggtt
     1321 acggcgcggc gatcgccatt gcgcatgcct cgaagatcgt caaggacaag agctatgaac
     1381 agtctgccag gcacggcggg ttcaccctga acgcgatcga cgaggcgaaa gcgctggcca
     1441 gaaaaaaccc cggcatttcg gaggaaacgt tgacagcagg catgctgtct tcggcgcccg
     1501 cgattcccga tgacgatctc gataccatcg ggctgcaatg gctgctggtc gcgcagtcca
     1561 ggatgtcatc gacgacagcg ggagacctcg cccgcacgat ctacgaaaac aaggccgaac
     1621 tcgcgctggc cgatggcttc gcctccagga tcgagccggc cgacaccgac aaggacgcct
     1681 tcatcgtcgc gcaccagggc gccgccgaat acatcaacga cgacaccaag tcgttcatgg
     1741 accgttacag cgacctgctg tatctcggcg ccgccgcgct cagcgtgatc ggctcgatct
     1801 ttgccgggat ctacaccaag ttcacccgcg tcgcgccgga gaaagccggc gagcttgcga
     1861 ccgcgatcct cgacatcggc gagagggtag aacacgccga ttcgctggac cagctcgaag
     1921 cgttgcagga cgaactcgaa gccattcttc gcggcgcggt gatcggattg cgcgacggca
     1981 ccatctcgag caacgggctg gatacgttca agctcggcta cgaattcgtg cgcgacggga
     2041 tcggcatgcg ccgcgatcac ctgaagcgca acgccggtca tgacggcaat gtggtagtcg
     2101 tcaagtcagc gcaaagcgca taggtgaatg gtgaatggcg agtagcgaat ggcgagtaga
     2161 gagggaaaac tattcactac tcgccattcg cctctatcct cgcgcccgcg ccctgatctc
     2221 cgcgatggtc cgcttgagcg cggcctgccg cggatcgggc gtgaccgcgg cgcgggcctc
     2281 cgccacggcg gcggcaagat cagctaacgg cagattctcg tcgagggtcg gcttgacgag
     2341 gccatcgatg atcgcgtgcc agtcggcgag cgcctggcga taggcgtcgc cgtagccccg
     2401 caccatggtc gcggtctgca cgatcgtgga cgctgccgct ggctgcttgg tcaggctgcg
     2461 atcgatcatg tgcagccagc gttcgaccca gacccgctcc ttggcatagc gcaccgagaa
     2521 cagccgccat cgccgcaagc ccgcctcgat cctcaaacgg cgaatgcccc accggctcgc
     2581 attgccgaag cggatcgaga ccggcatatg cagccacccc acccattcga gggcatcgag
     2641 tgccggttcg gcgacaacgg cgggaagcgc cccgatcaat tcatcgagcc ggaatttcct
     2701 gacatcgtcg gcaaactgaa cattcggagc gccggaggcc atttcggttt cggcgagttt
     2761 tgactgcgcg atccggatcg gatcctcata gctcatgcgc atcgccatca gccgcgcgat
     2821 ttcgccgaac atgacgtcat cgacgccgcg gcggccgacg aaccgccgca accgctgcac
     2881 ataaaggccc gcatagctgg ggccctggta gtcgatcaag agatgaatgc cttcgctgac
     2941 gatcggggcg gcaccctcgg gcaggccgtt gggcaaaaac ggcggcgcct cgtcgtcgtc
     3001 gccgatgacg tcggcgagca aataacgcag cgaagacagg acgcccgcca ctgcagcgct
     3061 cccggccctt aagcgggatt cggtgcagcg gccgcggcgt gatgcgccag ccgctgctcc
     3121 agcgttgcga tattctgaaa cagccgggcg ctggcgagac gaagaaagta aaagccgaag
     3181 gccggattct gcacgtaaag ttcctcgacc ttggtgtagc tgacgctgag gatgagaccg
     3241 gcttcgatgc attccagcgt ctgggttcgc atgttggacg gcgacagcat gccgagctcg
     3301 ccgacgatgg cgccgaccgg cagttcgata cctgattcca ccagcttgaa tcggccgctg
     3361 acgatgtaga gcatttcctc ggccttctcg tccttgtaga acagcacctc gccggccgcg
     3421 cattggcgct cggccatgaa cggcttcagc cattccatcg acaggtcgct gttgaccgac
     3481 tttttcacct cgcgcaccaa ttgcagcatt tggtgcagcc ggtacgaatt gagcaccagc
     3541 agcaccgtct gcaacgcccc ggtcggataa ttgtgactgg gaatcgagga cgcgatcagg
     3601 acgacgttgg tgagaatgcc gaaaacccgc agcgggatca tcgtccgcat cgaaagggtc
     3661 gcgaccacga agactgacgc gaacaggctg gctgcggatc ctgcatgttc tgcgaaatga
     3721 gacgtatcca ttggaggcca accaattttc aggaagtaag ggtttttttt tgcgattctg
     3781 ttccagctag agcccccatc ataatgtgcg gggggagtct ttgatatacg gggggaaagc
     3841 gaggttttgt gagggattat ggctcatccc ggccaataac tccgatttgg ggagggccga
     3901 gtttccccgt ccccggcgat aacgctgttt tcgggctgtc gttgacgggc tgccaccgcc
     3961 cgggcacttt gtcggaccgg ccgcgcgcag cacgccgcat ccgggccttt taccgacgga
     4021 ctccgcctcg caatgttcaa gccgcttgct ttccctggtc tggcagaatt cgcggccgcg
     4081 accgccggcc gcaacatgac caaagcggcc tatgtcgcgg tcgcggccgg cgtcgccatg
     4141 atggttctgc tcacggtcga tcccgcctat gaaacggcgc atcactgggt cgacgggttg
     4201 ctttggggct gccttgcttt ctttgcattc gaatggatcg tccggcttcg ccacgccgtt
     4261 ctgacgcagc gtggatgggc ttatgcatta tccggccgcg ggctggtcga cgccgtcggc
     4321 gccctggcgg taccgctcgc cctcgtcctc ggcgcgaatc ccaagaccgc gtggttgctg
     4381 ggcatattct ggatgttcaa ggtagtcccg ggaattccgg gactacggca attgcgccgg
     4441 gtgctggtgc aggaatccgg gccgctgttc agcgtgctgg tgattttcct gatggtgctg
     4501 tttctcgcct cggtgtcggt gtatttcctg gaacgcgacg tccagccggg cactttcggc
     4561 agcgtgcccg cggccttgtg gtgggcgatc gcgaccctga ccacggtggg atatggcgac
     4621 gtggtgccga tcacgccgct cggccggctg gtggcggctg tggtgatgat ctgcggcctc
     4681 ggcgttttcg ggctctggac cggcattctc gccaccggct tcgccgccga gacccgccgt
     4741 gacaattttc tgaaaacctg ggagtcggtg agcaaggtgc cgttctttgc cgcgctgggg
     4801 cccgccgcga tcgccgatgt cacgcacatg ctgcgcacca tggatctgcc ggcgcgcacc
     4861 atgatcatcc gcaagggcca gaccggcgat tgcatgtatt tcatcgctgc cggcgaggtc
     4921 gaggtcgatt tgccgggcaa gaaggtgacg ctcggcgaag gcgcgttctt cggtgagatg
     4981 gccctgctcg gcaacaattt gcgctccgcc aatatcacca ccaagcgggt gtcgcggctc
     5041 ctggtgctgg atctcgtgga cttccgcatg ctgatggcgc ggcatcccga tctggccgag
     5101 accatcgacg tcgaggccag gcggcgcgag ctggaaaaca agtaacggag agacgcatgc
     5161 ccgaagcgac cgcaactgcg agcgcgccgt tgctcgatat cgatggcgcg cgcgccacca
     5221 tccgcctcaa ccggccgaga catttgaacc ggctgcaggc cgatgatctc gatgcgctgc
     5281 taaaactgtt cgaccggatc gaagccgatc cggccgtccg ggtcatggtg ctcaccggca
     5341 ccggccgggc cttcagcgcc ggctacgatc tcaactcggt cgccgagcgc gccaccagcg
     5401 ccatcgagca gaagagcgcc ggatcggcgt tcgaggccgt ggtcaaccgg ctcgaggatc
     5461 tcggcgttcc ctcgatctgc cgaatcaatg gcggcgtcta tggcggcgcg accgatctgg
     5521 cgctggcctg cgatttccgc atcggggtcg atacctgcga gatgttcatg ccggcggcac
     5581 ggctcgggct gcactactac aagagcggca tcaggcgcta tgtgtcgcgg ctcggcgccg
     5641 acaatgccag aaaactgttc ctgaccgcgc agaagattgg cgccacggag atgttgcgga
     5701 tcggttacct caccgcgatg gcgccgcccg aggcgctgga cgaggaagtc gacaagctcg
     5761 ccacgattct tgccggcaat gcgccggtcg cgatgcgcgg catgaagcgc accatcaacg
     5821 agatcgcccg cggcgcgctc gacgaagacg ccgccgacca gcggcatcgc gacagcatgc
     5881 gcggcgccga gatcagggaa ggtatcaagg cgttcgcgga aaagcgtccg ccgaggtttt
     5941 gattttctta cctcgccccg cttgcggggg gcgagggtca tcgctactcc tcgaagaacg
     6001 tcacggtatc ggcaacgctg gcgcgggtgg tgcggtagct gttggccttg tggtcgcggt
     6061 cggggaagcc gaatgaaatg ccgcacacca cccgccggtc atcgccgagc ccaaagtgct
     6121 tgcgcaccag ctcggaatga aacgccagcg ccgcttgcgg gatcgtggcc acgcccagcg
     6181 cctgcgccgc cagcatgaag ctcgtgacat agccgccgca atcgatcgcg ccgtaaacgc
     6241 cgagcgcttc gtcggtgtgt acgatggcga catgcggcgc accgaagaag ttgaagtttt
     6301 ccagcgcctg cttcgcatag cccgctttgt cacccctggg aatgccgagc gagttgtaaa
     6361 gctgaaagcc gctctcgcgg cggcgctcga gatagacccc gcgatattcg cgcgggaacg
     6421 gaaagtcgcc ggtattcggc ttgccgctgg atgcggcgcc atggatcgca tcgcgaaatt
     6481 tttgggtcgc cgcacccgac gcgatcgcga gctgccaggg ctggctgttg caccacgacg
     6541 cggttttttg cgccgccgtc aatatccgct cgatagtcaa gcgcggcacc gggtcgggcc
     6601 ggaacgcgcg gcaggaatag cgttcgctga gcaattcctc aagcacgccg atccgctgtt
     6661 cgacgctgtg ccggcttgcc gacgtcttca gggcagtatc cgacatgttc atcgcccctt
     6721 ggtcgggatc ttgttgaacg gcacattctt gtcgacgcgg atgtcgccgg gcaggcccag
     6781 cacgcgctcg gcgatgatgt tgcgcaggat ttcatcggtg ccgccggcga tccgcatcga
     6841 cggc
//
LOCUS       DATNOI010000090         5245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1393497,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000090 DATNOI010000000
VERSION     DATNOI010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393497"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..1470
                     /gene="mnmA"
                     /locus_tag="VN968_03090"
     CDS             265..1470
                     /gene="mnmA"
                     /locus_tag="VN968_03090"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXL28270.1"
                     /translation="MFNSLDLKGRPQDTRIVVAMSGGVDSSATAALLKSEGYDVVGIT
                     LQLYDHGAATHRKGACCAGQDIHDARDVAERIGIPHYVLDYENRFRESVIDNFADSYA
                     SGETPVPCIECNRSIKFRDLLATARELGASALATGHYVASRRLSDGSRAMVCAADADR
                     DQSYFLFATTREQLDYLRFPLGDMTKQQTRELARRFGLAVADKHDSQDICFVPTGRYT
                     DIISRLRPNAIEPGDIVDLDDRVIGRHQGIVHFTVGQRRGLGIASDAPLYVVRLDAVG
                     RRVVVGPREALRMDRIVLRDVNWIGDGALDRAVGDGLEIFVRVRSTRAPQPAWLRAAG
                     GGYEVELVAGEEGVSPGQACVFYDAPSGQARVLGGGFIQSATAKSATARAAVRDAIAR
                     QSLVEAMRG"
     gene            1488..2126
                     /locus_tag="VN968_03095"
     CDS             1488..2126
                     /locus_tag="VN968_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXL28271.1"
                     /translation="MASDIDRAGVARAYERWAPVYDLVFGKVFDHGRQSTIAEADKIG
                     GRILDVGVGTGLSLSDYSRTTKLCGVDISEPMLRKAQQRVRALGLGNVEALAVMDAKN
                     LAFADSFFDAVVAQYVITAVPDPEATLDDFIRVLKPGGELILVNHIGAESGPRRIFEL
                     SFAPLARRLGWRPEFPWARLADWAAKHGGVSLTERRPMPPMGHFSLIRYRKS"
     gene            2179..2544
                     /locus_tag="VN968_03100"
     CDS             2179..2544
                     /locus_tag="VN968_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28272.1"
                     /translation="MRIKHSLFLYCAMIAASGAASAQAPPTPATPPAQTAPPAPARST
                     NCAPMQPTPHQGAVTPEGTTTGQRAEALGDKLARSDGVLCPPAGVDPEIRAPTPETGN
                     TPVIPPPGSPGGDPTVRPK"
     gene            2662..2738
                     /locus_tag="VN968_03105"
     tRNA            2662..2738
                     /locus_tag="VN968_03105"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2696..2698,aa:Met,seq:cat)
     gene            2831..3124
                     /locus_tag="VN968_03110"
     CDS             2831..3124
                     /locus_tag="VN968_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28273.1"
                     /translation="MRLGAYDDPGHQPPHRFDQFRRFRKIAVLSGEGGQSFDIGAVLL
                     DRARMQCDAIGGTGALQLPGDIGALRFQRLDPRHQGSEVDALRYRVDQPLEFA"
     gene            complement(3263..4309)
                     /locus_tag="VN968_03115"
                     /pseudo
     CDS             complement(3263..4309)
                     /locus_tag="VN968_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(4448..4672)
                     /locus_tag="VN968_03120"
     CDS             complement(4448..4672)
                     /locus_tag="VN968_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28274.1"
                     /translation="MRPVDSRRVDHAPELRVLFFPPTSAVGALERPLAVLGIAWIGDD
                     VVDHLVANNKAIRIGDWVILAMVPSDASGR"
BASE COUNT          933 a         1656 c         1653 g         1003 t
ORIGIN      
        1 gaaactacgg agccggcgcg gcaaccgcct tttctgcccg gcggcaagat ttgccgtccg
       61 cagcgtgcaa aacaggctcg gctgattgct tcgcgggaat acggcctata ttaaagagca
      121 gctttacagc cgttccgcca ggccgaagcc ggtccgcggg acgctgcaag gcattctgac
      181 tgcaaggctt tttgactatt cgcaaagcgg cgttctaatc gcgaagcgcc cgatgccgcc
      241 gcgatcgcac cgatgaccgg aaccatgttc aacagtctgg atctcaaagg ccgtccacag
      301 gacaccagga tcgtcgtcgc catgtccggc ggcgtcgatt cctcggcgac ggctgcattg
      361 ttgaaatccg agggctacga cgtcgtcggc atcacgctgc agctttacga tcatggtgct
      421 gccacccatc gcaagggcgc ctgctgcgcc gggcaggaca tccatgacgc acgcgatgtc
      481 gccgagcgga tcggcattcc gcattacgtg ctggattacg aaaaccgttt ccgtgaatcg
      541 gtgatcgaca atttcgccga cagctacgct tccggcgaga cgccggtacc ttgcatcgag
      601 tgcaaccgtt cgatcaagtt tcgcgatctg ctggcgaccg cgcgcgaact cggcgcttcg
      661 gcgcttgcga ccgggcatta tgtagcttcg cgccgcctga gcgatggatc gcgcgcgatg
      721 gtttgcgccg ccgacgccga tcgcgatcag agctatttcc tgttcgcgac cacgcgcgag
      781 caactcgatt atctgcgttt tccgttgggc gacatgacca agcagcagac gcgcgaactg
      841 gcgcggcgct tcggccttgc ggtcgccgac aagcacgaca gccaggacat ctgcttcgtg
      901 ccgaccgggc gttataccga catcatcagc cggttgaggc cgaacgccat cgaacccggc
      961 gacatcgtcg acctcgacga tcgtgtcatc ggcaggcatc agggcattgt tcatttcacc
     1021 gtcggccagc ggcgaggatt gggcatcgcc tccgatgcgc cgctgtatgt ggtgcggctc
     1081 gacgctgtcg gccgccgcgt cgtggtgggc ccgcgcgagg cgttgcggat ggatcgcatc
     1141 gtgctgcgcg atgtcaactg gatcggcgac ggcgcgctcg atcgtgccgt cggcgacggc
     1201 cttgagattt tcgtgcgcgt gcgatcgacc cgcgcgccgc agccggcgtg gctgcgcgcg
     1261 gccggcggcg gttatgaagt cgaactcgtc gccggcgaag agggcgtatc gcccggccag
     1321 gcctgcgtgt tctacgatgc gccgtcaggc caggcgcgcg tgctcggcgg cgggttcatc
     1381 caaagcgcga ccgcgaagag cgcaacggcg agggcagcgg tgcgcgacgc tatcgcccgg
     1441 cagtcgctcg tcgaggcaat gcgcggataa aatttcaggg cgggggcatg gcttcagata
     1501 tcgaccgcgc tggggtcgca agggcttatg agcgctgggc gccggtctac gatctggtat
     1561 tcggcaaggt tttcgatcac ggccggcagt cgaccattgc cgaggccgac aagatcggcg
     1621 gacggattct cgatgtcggt gtcggcaccg gattgtcgct gtcggattat tcgcgcacca
     1681 cgaaattgtg cggcgtcgat atttccgaac cgatgctgcg caaggcgcaa cagcgcgtgc
     1741 gtgcacttgg gctcggcaat gtcgaggcgc tggcggtcat ggacgccaag aacctggcgt
     1801 tcgcggattc gtttttcgat gcggtggtgg cgcaatatgt catcaccgcg gtgcctgatc
     1861 ccgaagccac gctggatgat ttcatccgtg tgctgaaacc cggcggcgaa ctcattctgg
     1921 tcaatcatat cggcgccgag agcggcccgc gccgcatctt cgaactgtca ttcgcgccgc
     1981 tggcgcggcg gctcggctgg cggccggaat ttccctgggc gcgtctggcc gattgggccg
     2041 cgaaacacgg cggcgtcagc ctgaccgagc gccgcccgat gccgccgatg gggcatttct
     2101 cgctgatccg ctatcgcaag tcgtgagatt gcggaatgtc cgggcggaac ttccgtccgc
     2161 gtagcccgtt ttatcgggat gcggatcaaa cactcactct ttctctactg cgcaatgatc
     2221 gcggcatccg gcgcggcgag cgcccaggct ccgcccacgc cggccacgcc acccgcccag
     2281 accgcaccgc ccgcgccggc gcgctccacc aattgcgcgc cgatgcagcc gacgccgcat
     2341 caaggcgccg ttacgccgga aggtacaacc accggccagc gcgccgaggc gcttggcgac
     2401 aaactggcaa ggtccgacgg cgtgctgtgt ccgcccgcgg gcgttgaccc cgaaatccgc
     2461 gctccgacac cagaaaccgg caacaccccg gtgattccgc cgcccggcag ccccggcggc
     2521 gatccgaccg tccgcccgaa ataactacgc tttgcaatca cttggcggaa agggcgggtt
     2581 cgcttgacat gcccgtgacc ggcttcttat atgccgcgcg ttcgcgactg cggtatatag
     2641 cctcaagacg atggacgccg tggcggggta gctcagctgg ttagagcacg ggaatcataa
     2701 tcctggggtc gggggttcga gtccctctcc cgctaccacc aatcccccct ccctcagaac
     2761 ggctcgggaa caaagccgaa ttcgtcaatt gtgccagcct tccctgcact tcgacgctga
     2821 agcctcggcc gtgcggctcg gcgcatacga tgaccctggc caccagccgc cgcaccgctt
     2881 cgatcagttc cgccgattcc gcaaaatcgc tgtgctcagc ggcgagggcg gccaatcctt
     2941 cgacatcggc gcggtattgc tcgatcgcgc caggatgcaa tgtgacgcta tcggtggcac
     3001 cggcgcactg cagctgcccg gcgatatcgg cgcgctccgc ttccaacgcc ttgatcctcg
     3061 gcaccagggt agcgaggtcg acgccttgcg ttaccgcgtc gatcagccgc tcgagttcgc
     3121 gtgagacctc ggcattgcgg cgtgtcaact tgctcatctg gtcgccagct tcgcgcgcca
     3181 ggcgccgacg ctcctcgtta tagaccttga cgtattccgt aatcgcaatc ggattggcca
     3241 gttcgtcctt cagtcccgcg aatacggccg cttcaatggc gtcgcggttg accttccggc
     3301 tgttggtgca ggatccactt tccttgtgtg tgccacattg cagtcgtgcg gtccgtttgt
     3361 gattgccgat cgataccatt ccgccgccgc aggatccgca ccgcagcaac cccgagagcg
     3421 gacgccgcgg cgcgcgggac tgaggtgcgc cgaggtgcga gcgcttcagc ttgatggcct
     3481 gcgccgcgtt ccagctcgca tcatcgataa cgcgcagttg cggcgcctcg gtaatgcgat
     3541 gctgctcctt cgcattggtc cgcgaaatac gcttgcccgt ggttggatcc ttgaccatcc
     3601 ggaccttgtt ccagacgatg cggccgacat aaagctcatt gagcagcatg ccgtggcccc
     3661 gcgcattgtt gccattgata gtggatgcgt tccatctggc gccgcgcggc ggcgctatgc
     3721 gatcggcgtt gagttcggca gcaatggcgc gaggcgtggc gccggcaaca aagcgtgcga
     3781 aaatccgtcg cacgatctcg gcttgcgcgt cgtcgatgac gagctcgccc ggcgaaccgg
     3841 ggacgatgga atagccatag gcacgtccgc cggcgtggcg gccgtcgcgg atcacgccct
     3901 cgagtccgcg tcgtacatgc agtgccaagt tttcgagata catttcgccc atcagcgccc
     3961 gcagcgcgcc gtcgatcttg gtcaccttac cggttgcggt atgaatggtg atgccgagaa
     4021 aatcgagttc cttgcgggca gcgtgataat cggcctggtc gcgaaaaatg cgatccatgt
     4081 cttcggcaac gacgacgtcg aacagctttg ctgctgcagc gcgcatcagc gcaatgaagc
     4141 cgggacgatt ctttgctgac gacgcggaca cagcgcggtc ttcgaaggtc aacaccacag
     4201 tcatgccatt gtgagcgcag acatcgcggc agagcgcgat ctggtcatca actgatctgt
     4261 cgttttggag atcgctggaa aagcgggcgt agatggcgac gcggatcatg cactcggctc
     4321 ggtcgcgcgg gcttgaactg atctgcgcgc gatttcggct tcatgatcta tccgagcgcg
     4381 cagtctggca agcgctctga ccacggcgag aacgtcctcg tcgagcggat tttgcagttt
     4441 ttgtggatta acggccggag gcatcgctcg gaaccatggc gagaatgacc cagtcgccga
     4501 tccggatggc cttgttgttg gccaccaggt ggtccaccac atcgtcaccg atccaggcga
     4561 tgccgaggac cgccagcggc ctttccagcg ctccaacggc actagtcgga gggaagaaaa
     4621 gcacgcggag ttcgggtgcg tgatcgacgc ggcgcgaatc gaccggcctc atgctgcacc
     4681 gtcggttgac gaaaaagcgg ccccccgctt ggcggagcag ccgccgcagt gaccggcgcg
     4741 ggagtgatcg ctggattgga gccgacgcgt cgggcctgcg tcagcgcggc gtcgatcagt
     4801 gcgacgatat cgccgacgcg ccggttcttg atccgcggcc aatgaagtgg tgaagctacc
     4861 aaccatgcgt gcaacatgcc gccgccagca catccacgaa agggcggctt accttgcgtc
     4921 gcaaagacgg aaagcttagc gcgtcaatgg gtggtttcgt ccgatggtgc gacctttcgc
     4981 taatgcgccc aatagctcaa aacaaattcg tttcagggta tcgtcggaat cagcccagac
     5041 ttaaaatcat ttcagtcggg gtcctgatcc ggagaagttt tctagcggta ggaccggcgg
     5101 ccaaaattgg tcgctcgtga cgagcggttc gatgccgccg ccagtcggtc gtcaggctca
     5161 taacctgaag gtcacaggtt caaatcctgt ccccgcaacc aaacaataag cccgctaggt
     5221 caatcgctta gcgggctttt tcatg
//
LOCUS       DATNOI010000091        17936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1222907,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000091 DATNOI010000000
VERSION     DATNOI010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17936
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222907"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VN968_03125"
     CDS             complement(<1..1051)
                     /locus_tag="VN968_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXL28275.1"
                     /translation="MKNFDYFRPATISDAVAAAAEPGAAYLAAGTNLLDLMKGGVTGP
                     SRLVDVTRLPGLDRIERLPDGGIRIGALVRNADLAHDPDFAKAYPAIAEALLSGASAQ
                     LRNAATVGGNLLQRTRCGYFYDIASACNKREPGTGCEARGGENRLHAVLGWSEACIAT
                     HPSDFCVPLVALDAVVEIEGRNGKREVAVEALHRLPGDTPERESVLEPGDLIVALRLP
                     GEARAFSAHARYLKVRERTSFAFAVVSAAAGLRIEQGAIRDARLALGGVAAKPWRARA
                     AEEMLAGSSPGPDAFHRAAKAALADAKPSGDNQFKIELAQRILLRALILAAAGTPQRI
                     PALPASPFASVPGALH"
     gene            complement(1048..1545)
                     /locus_tag="VN968_03130"
     CDS             complement(1048..1545)
                     /locus_tag="VN968_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXL28276.1"
                     /translation="MNLSISLTVNGARRDIELGDPRVTLLDLLRERLHLTGTKKGCDR
                     GQCGACTILVDGRRINPCMALALSHDGADVVTIEGVSRGDQLHPVQAAFIAHDGFQCG
                     FCTPGQIMSAIGLIGEAQAGDDPERVREGMSGNLCRCGAYSGITDAVLDVQQSLIEAD
                     QRRSA"
     gene            1803..2474
                     /locus_tag="VN968_03135"
     CDS             1803..2474
                     /locus_tag="VN968_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL28277.1"
                     /translation="MADQSAATVRRPRADAVRNRERVLEAAKAVFSAGGPDASLEAVA
                     KRAGVGIGTLYRHFPTREALFEAVYRREVQQLGDLAEQLKSEAEPVDALRRWLRSNVE
                     FVATKKGMSAALALAVQSSSELTAYSFDRLAKAVGGLLDRAVLAGEIRSDISPEDLLR
                     ALVGMCYMHDQPGWQKSVLRLLDVFVDGLRVQANVDGKTASVPRRRDTTARAPGRTGS
                     RKSRK"
     gene            2602..2952
                     /locus_tag="VN968_03140"
     CDS             2602..2952
                     /locus_tag="VN968_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28278.1"
                     /translation="MLAHQPFGAPGFAGDDRFINAVMIVVAAAQVTMLEGDDVAARRY
                     RGAMASPYDLAEHAVARDGRHRVMEIAVDAAIDRQIAGFERALLQQPVAGGEAAFDGS
                     LRFRRLPGCEIQPD"
     gene            complement(2940..3239)
                     /locus_tag="VN968_03145"
     CDS             complement(2940..3239)
                     /locus_tag="VN968_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28279.1"
                     /translation="MPQIWMTYEELASMLDCTVAEARERAHLERLDRKMSHDGKPRAK
                     LSLAMTGIFIERLKTIDYDIDRVVNNLRLVHGLLSECVDQSAHLAPQRRRSHQSG"
     gene            3519..3704
                     /locus_tag="VN968_03150"
     CDS             3519..3704
                     /locus_tag="VN968_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28280.1"
                     /translation="MAKKAKKTVLRREYTKADVKELRTHSKAKTPVAKIAKLTKRTEG
                     SLRQKALKLGIRLGHRR"
     gene            complement(3847..4106)
                     /locus_tag="VN968_03155"
                     /pseudo
     CDS             complement(3847..4106)
                     /locus_tag="VN968_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011648945.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(4145..5335)
                     /locus_tag="VN968_03160"
     CDS             complement(4145..5335)
                     /locus_tag="VN968_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HXL28281.1"
                     /translation="MTSTETISFELSQPALQARRNAKWNQYDADILPAFVADMDFSVA
                     APIQSAIERIVRDRDYGYALRNGEKPDRLVAGAFAQRMKSRYGWELSPDLVLPLADLV
                     QGTYAPILAFSEPGDGVVLQTPNYPPFRDAINSTERELLALPMRDDGTRFVFDMNELE
                     KLVNKRTRIFVLCNPQNPTGRVFSRDELLALGQFAIEHDLVVISDEIHSDLVFPGLQH
                     IPFASLGPEIAARTVTLNSATKSFNIPGLRCALMAFGAEDLRDRFHKRIPQRLTGSAN
                     IVGVDATVAAWSECQPWLDAVMEYLLKARNRVKDVLAAEVPEIRFHAPEATYLGWLDC
                     SKLGLSTPAFQFFLNNARIGFSAGETFDPGCAQFVRFNFATSMPILDDILDRFVAATR
                     QNDR"
     gene            5693..6535
                     /locus_tag="VN968_03165"
     CDS             5693..6535
                     /locus_tag="VN968_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXL28282.1"
                     /translation="MSTALRIVHGNFGRVALLDMDCSLVRHAHPHCHVLFKVAGADTQ
                     FVVGNRIHALTDSAAVLVDGWQPHSYVHDPARPRTLILALYVEPEWLKAFRPGWAASG
                     APGFFETPHGEVSPGIRTLTMDLASEMMADPECQAEYEALLSELMIAVIERFTPWKSF
                     PRSIRELNVPACDRRIRRAAEYIRSNAGGEASIDTLAKEAGLSRAHFFRLFQSSIGLP
                     PKVYLNVVRMERAVEAVLHRQVPVGELGARLGFSDPAHFTRFFRNNAGVSPSEFRQVS
                     RLAG"
     gene            6667..9660
                     /locus_tag="VN968_03170"
     CDS             6667..9660
                     /locus_tag="VN968_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL28283.1"
                     /translation="MLHAAGPAIAESKAARPWVGQSIPRVEDAALLTGKGRFIDDLGV
                     RPGTLYAAILRSPHAHAEILSIDGEAARRADGVVAVLTGDDIKVLTTSLVVGVKAKVE
                     CWPIAVGRVRYVGESVAVVVASDRYKAEDAVDLIDVQYRALPANIDPLQALSPSSPVI
                     HDGFDGNVASDRTFRYGNPERAFAKAAHRISIDIRYPRNSCTPIETYGVIADYDASED
                     AFDILANFQGPFSIHAVISRALKVPGNRVRLRTPPDSGGSFGVKQGVFPYIVLLAAAS
                     RAVGRPVKWIEDRLEHLTASVSATNRATTLSAAVAADGRILALGWDQVEDCGAHLRAP
                     EPATLYRMHGNLTGAYDIPNVAVRNRVVVTNKTPSGLNRGFGGPQVYFALERLMQRIA
                     VELKLDPLDVIRRNLVPADRFPYRTATGALLDSGDYREAIARGVKGGALDELKARRDV
                     ARAEGRLYGIGYAAVVEPSVSNMGYITTVLTAAERKKAGPKNGAQATATVALDPVGSV
                     SVHVASVPQGQGHRTVLAQVVADVFGLTPADVRVNTEIDTAKDAWSIASGNYASRFAA
                     AVAGTAKLAAERIAARLARVAASQFNTDADDIVFADGAVSSRRNPDNRLAFSRLAALS
                     HWSPGTLPDDVQQTIRETVFWTSPELTPPDDEDRINASLCYGFIFDFCGVEIDRTTLM
                     TRIDRYVTMHDCGTILHPGMVDGQIRGGFAQALGAALYEEYAYAADGTFLTGTFADYL
                     LPTTVEVPDPVILHMETPSPFTPLGSKGVGEGNCMSTPVCIANAVADALGVNDVELPL
                     VPAKLADIVRGAEPPPPAGRRADVPKSANGGRKLRGEGSATVAASPEAVWTMLLDPTT
                     LQAVIPGCHGVEKISDTHFRAEVTIGIGPVKGRYRAEVTLSDLDAPRAVTLGGFAEGA
                     LGLGKGEGRITLTPEQAGTVVRYEYDAAIGGKVASIGGRLLDGAARVIIGQFFSGLAR
                     QAGGTSAQGRPSLLGKLLSLFGRRP"
     gene            9657..10472
                     /locus_tag="VN968_03175"
     CDS             9657..10472
                     /locus_tag="VN968_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HXL28284.1"
                     /translation="MKPAPFDYVRAETLDEALAVLASEGSDAQIMAGGQSLMAMLNTR
                     LAKPKVLVDIMRLKELDRIDATSAAVTVGAGVRQRSLLGWPPLAEKLKLVALALPWTG
                     HAQTRSRGTICGSIAHADPSAEMPLALLALGGEVHLRTASKRRRVAAEKFFTGMMSTA
                     RAADEMIEAVSFPYLGVARCAFREVARRHGDFAIVACAAVASRQGVRFAVGGVADMAA
                     VRDWPRLDGTALDDALNAFAHELDARDDVHATARYRRDLVRLIGRDLVREVLQ"
     gene            10469..10993
                     /locus_tag="VN968_03180"
     CDS             10469..10993
                     /locus_tag="VN968_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL28285.1"
                     /translation="MTRLESDRQHRISFTLNGAAAQGEAEPRMLLTDFLRHQIGATGT
                     HVGCEHGVCGACTVTVDGMIARACLTLAVQIDGSDVRTIEGLAPAPDRLGILQAAFKR
                     HHALQCGFCTAGILMSLDHYLRERPKPTEAELRDFLSGHLCRCTGYTPIIRAALDAAD
                     LLANQPRAKMKTDV"
     gene            10986..12539
                     /locus_tag="VN968_03185"
     CDS             10986..12539
                     /locus_tag="VN968_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXL28286.1"
                     /translation="MFDLGTSFVASVARDPGAVAIVDAGLRLTYAQWFARISSVVAAF
                     DDFGLRPGDHIVTVLQNRWEAATIHWACQFAGLVITPINWRAKSDELDFCIENSQAQA
                     LVYESVSAGAVSGARFARNLRRIVVGDAVAADDQAVLFATMIDTPAPEAVPRGGPEAW
                     SIMLYTSGTTSKPKGVPRRHRAERAAGVAHVAQNLYGRYERTLGVMPLYHTMGVRSLI
                     SMSLIGGTFVCLPRYDPGLALAIIEAEKITNLYLVPTLYHDLVNHADFEKTNTSSIRK
                     LGFAGAPMTDGLLQKLNSAFKPDLFVNHYGSSEIYTFTVNQNAVAKPGSAGKAGINQF
                     VRVVKLAARSASELASTEEEGEIIALLASDESFEGYWRRPDADSKSLREGWYFTGDTG
                     FVDKDGDLFVTGRTDDMIITGGENVSPVEIESCLSLHPLVSEVAVVGLADERWGKIVA
                     AFVKRSGYVSEADLEAFCRDSGLANFKRPRKFVFVDAIPKSPVGKLLRRLLVAGEYDV
                     ERRPPSTAA"
     gene            12571..13377
                     /locus_tag="VN968_03190"
     CDS             12571..13377
                     /locus_tag="VN968_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXL28287.1"
                     /translation="MPSSFNFADPRLSKLDGFQVEIDETHERADIILNRPPYNIVSMP
                     QRDQLRLVFETLDEDSRVRVIVVRSIGEHFSSGGNIGGFMEATPEHVSKLAWNIASPA
                     RCAKPVIVANRGYCFGVGFELSLACDFRIASETTFYALPEQKLGQIPGSGGSARLQKM
                     IGITRTKDIVMRSKRIAGQQAQEWGIATECVPDAELEKATDKLVDELRTFSPLAQRTA
                     KKLLNDTEDSTLAIAIELEGHCYSRLRQSSDFKEGVEAFNAKRAPNFTGL"
     gene            13488..14819
                     /locus_tag="VN968_03195"
     CDS             13488..14819
                     /locus_tag="VN968_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL28288.1"
                     /translation="MTTTSAPLVDAQAPADNRQMINAIIASLLGWSLDLFDLFILLYV
                     APVIGTLFFPSTNATLSLAAVYASFAVTLLMRPIGSAIFGHFADVHGRKGAMLVAIVG
                     VGVSTAAFGALPTIAKVGIAAPIMFLILRLVQGIFVGGVVASTHTIGTESVPANWRGA
                     MSGLVGGGGAGIGALLASFVFLIASSVFPGEAFAQWGWRFMFFSGLLSSLLGWFIFKN
                     LEESPFFNELQQQKAARKAMAAPSPVRVLFSSGYRSVLLINLLITFGGGAGYYLTSGY
                     LPSFLKVVNGVPNNVSSLILIGASVVAFISAVLFGALSEMIGRKPTFLIVGVCALVWL
                     PLCYLGLAKATDIKEVTIYALAIAFIGNAGYAPVLVFLNERFPTSLRASGTGLSWNIG
                     FALGGMMPTFVSLASAGTAQIPTSLAIFSVAIYLIYLIGALIIPETRGNFR"
     gene            15174..15635
                     /locus_tag="VN968_03200"
     CDS             15174..15635
                     /locus_tag="VN968_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28289.1"
                     /translation="MLAALLVGKALKTPVALAAVTPIDETTIGIGSAQDPLTKADRLE
                     FAGVRPEIPAQFALRPTVPLTPPAPTSVSPVETKIVSRDWHDPNATASLAGKSKQPAR
                     MATGKKAKSVDAKGRRTADRSKPAGHGKPCQRTGALGDFLKSLKLSPACVS"
     gene            complement(15750..16382)
                     /locus_tag="VN968_03205"
     CDS             complement(15750..16382)
                     /locus_tag="VN968_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007796851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28290.1"
                     /translation="MTGAWPWLALTGLGAFHGLNPAMGWLFAVALGLHRRSRATVLLA
                     LPPIAFGHAVSIAMVAGAFVAAGVLVNQRAIQVSAGLLLIGWSVYHQLYGHRHRVRVG
                     MKTGQAGLILWSFLMATAHGAGFMILPAMIPLCLASSSAGETGASGPAATALAAVAVH
                     TAAMLVVTGAVAVIVYEWVGLSILRTAWLNPDLLWTGALAGTGLVLLLLA"
     gene            complement(16429..17820)
                     /locus_tag="VN968_03210"
     CDS             complement(16429..17820)
                     /locus_tag="VN968_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding family protein"
                     /protein_id="HXL28291.1"
                     /translation="MTWRPDPSFYPSPRMAMKAPPEKFAYVAAFDPTRKKPDAIAVVD
                     VDPGSKSYAKILGNVEMPSVGDELHHFGWNACSSCLCPNAPHPHVERRYLVVPGLRSS
                     RIHILDTKPDPRKPKIVKVIEPEEIAEKTGYTRPHTVHCGPGGIYVAALGNRDGKAPG
                     GIFMIDHESFEPLGRWEVDRGPQRLSYDGWWHLGYDTLVTSEWGTPDTFENGLVPEVL
                     LGGKYGRRLHFWDFTKRKHLQEIDFGPEYQLVFELRPAHDPTKAYGFVNCVISLKDLS
                     SSIWTWHRDGDKWAVTKIIDIPAEPADPDKLPPILKGFKAVPPFVTDIDLSMDDRFLY
                     VACWGTGDLQQYDVSDPFKPKLTGKVRIGGVVSRASHPGAKNGALSGGPQMVEISRDG
                     KRVYFTNSLYGTIDSQLYPDGLDGWMVKLDAKPEGGISFDEKFFVEWPKSHRPHQVRL
                     EGGDCSSDSYCYP"
BASE COUNT         3261 a         5522 c         5748 g         3405 t
ORIGIN      
        1 catgcaaggc tccgggaacg gatgcgaaag gggaggccgg aagagccgga atgcgctgtg
       61 gcgttcccgc cgcggcgagt atcagggcac gcagcaggat ccgctgcgcg agctcgatct
      121 tgaactggtt gtcaccggaa ggcttggcgt cggcaagtgc ggccttcgcc gcgcggtgga
      181 aggcatccgg tccgggactg gagcccgcca gcatctcctc ggccgcgcga gctcgccacg
      241 gtttggcggc aacgccgccg agcgcgagcc gcgcatcccg gatcgcgcct tgttcgatcc
      301 gtaagcccgc ggcggccgac acgacggcga aggcgaagga ggtgcgttcc cggaccttga
      361 gatagcgcgc atgcgccgag aacgcgcgtg cctcgcccgg cagccgcagc gcgacgatca
      421 agtcacccgg ctcgaggacg ctttcgcgct ccggcgtatc gcccggcaga cgatgcaggg
      481 cctcgaccgc gacctcgcgc tttccattgc ggccttcgat ctcgacgacg gcgtcgaggg
      541 cgaccagcgg cacgcagaaa tccgacggat gggtggcgat acaggcctcg ctccagccca
      601 gcacggcgtg caatcggttc tcgccgccgc gggcttcgca acccgtgccc ggctcacgtt
      661 tgttgcaggc gctggcgatg tcgtagaaat atccgcatcg ggttcgctgc agcagattgc
      721 cgccgacggt cgcggcgttg cgaagctgcg ccgatgcacc ggacagcagc gcctcggcaa
      781 tcgcgggata ggcttttgcg aaatccgggt cgtgcgcgag atcggcattg cgaaccagcg
      841 cgccgatgcg gatgccgcca tcgggcaggc gttcgatgcg gtcgaggcct ggcagacggg
      901 tgacgtcgac gagacggctc gggccggtta ctccaccctt catcaggtcg agcaggttgg
      961 tgccggccgc gagataggca gcacctggtt cggccgcagc ggcgacggcg tcggagattg
     1021 ttgctggcct gaaatagtcg aagtttttca tgccgagcgc ctctggtccg cctcgatgag
     1081 gctttgctgc acgtccagca ccgcgtcggt gatcccggaa taagccccgc atctacagag
     1141 gtttccgctc atgccttcgc gcacgcgctc aggatcatcg ccggcttgcg cttccccgat
     1201 caggccgatc gcactcatga tctggcccgg cgtgcagaat ccgcactgga agccgtcatg
     1261 cgcaatgaag gcggcctgga ccggatgcag ctggtccccg cgagagacac cttcgatcgt
     1321 gacgacgtcg gcgccgtcgt ggctgagcgc caaagccatg cacggattga tccgtcggcc
     1381 gtcgaccagg atcgtacagg cgccgcattg gccgcggtca catcccttct tggttcccgt
     1441 gagatggaga cgctcacgca ggagatcgag cagcgtgacg cgcgggtctc ccagctcgat
     1501 gtcccgtcgc gccccgttaa cggtgaggct gatggagagg ttcatgcagt gctccggtca
     1561 atgctacggt caaactgcat cgcggccgcc gggatttgat cgacgggcac gtgcaagttc
     1621 ggggctgatc gccatatacg gaggcatcct ccgttttgca agacaagaag ttttgcaaga
     1681 cgaggacaac cagagcatga tccggaaaat ggcaattgat tttcacaaaa gatcatgttc
     1741 aaacaaagag atgagatgat gatccgattc aatctaaccg gatcgtgatc caaaataatc
     1801 agatggcaga tcagtccgca gccactgttc gcaggccccg cgccgatgcg gttcgcaacc
     1861 gcgagcgcgt cctggaggcc gccaaggccg tcttcagcgc cggtggcccg gacgcgagcc
     1921 ttgaggctgt ggcaaaacgg gccggcgtcg gcatcggcac gctttaccgc cacttcccga
     1981 ctcgcgaggc cttgttcgag gcggtctatc gacgcgaggt gcagcagctc ggcgacctgg
     2041 cggaacagtt gaagagcgag gctgagccgg tcgacgcctt gcgccgctgg ctgcgatcca
     2101 atgtcgaatt tgttgccacc aagaagggca tgtcggcagc gctggcgctt gccgtgcaaa
     2161 gctcgtcgga actcaccgcc tattcgttcg atcgattggc aaaggcggtt ggcggcttgc
     2221 tggaccgcgc ggtcttagcc ggcgagatac gttccgatat cagccccgaa gacctgctgc
     2281 gggcgctggt cggcatgtgc tacatgcacg accagcccgg ctggcagaag agcgtgctgc
     2341 ggctgctcga tgttttcgtc gacggccttc gcgtgcaggc caacgtcgac ggaaagacgg
     2401 cgtcggtgcc gcgccgccgc gataccaccg cgcgggcgcc tgggcggacc ggatcccgca
     2461 agagccgcaa gtagatatcg ggcaatgatg atacgggagc cgaggttgtg ccgtcgccaa
     2521 aaccagtcga cggttcgtcc gttttgatcc cccgttgcgc gcggccagcg ccctaatcaa
     2581 cgagcccttg acgctcgaga tgtgctcgcg catcagccat tcggcgcgcc agggttcgcc
     2641 ggcgatgatc gcttcataaa tgcggtgatg atcgtcgtgg cggcggcgca agtcacgatg
     2701 ctcgaaggcg atgatgttgc ggctcgacga taccggggcg cgatggcaag tccttatgat
     2761 ctcgccgagc atgcggttgc gagagacggc cggcaccgtg tcatggaaat cgccgttgat
     2821 gccgcgatag atcgtcagat cgccggcttc gaacgagcct tgctccaaca gcctgtcgcc
     2881 ggcggcgagg ctgcgttcga tggcagcctt cgcttccgaa ggctgcccgg ttgcgaaatt
     2941 cagcccgatt gatgcgaacg gcgccgttgg ggcgccaggt gtgcggattg gtcgacgcac
     3001 tcactgagga gaccatggac aagtctgaga ttgttaacca cacggtctat gtcataatcg
     3061 attgtcttga gccgctcgat gaaaatgccg gtcattgcca ggctgagctt ggcgcgcggt
     3121 tttccatcat ggctcatctt ccgatcgagg cgttcgaggt gcgcccgctc acgtgcttcc
     3181 gccacggtgc aatccagcat gctcgccagt tcttcgtacg tcatccaaat ctgaggcatg
     3241 gctgtccctt ggaggatttg atagaggcta tttgctcgaa gccgagaact gctgttcttg
     3301 gtacaaatct tggcacaaac attttaagtt tgggttagtg gagtgcctgt gaccgatagg
     3361 ccagcagcct tcacgaaaat tgacggtgga gaggcggctt cccggatcta gatccttacc
     3421 agccggccaa agcttgcgat ccggaatgtg ctgcaagttg gagtgggcgg ccccgaaatc
     3481 tgtgctagaa gagcgattcc aaaaatcagg agtaccgagt ggctaaaaaa gcgaagaaaa
     3541 ctgtcttgcg ccgcgaatat acaaaggcgg atgtgaaaga gttgcggaca cactcgaagg
     3601 cgaaaacacc ggtcgccaag attgcaaagc tcaccaagcg taccgaagga tcgctgcgac
     3661 agaaggcgct caagcttgga atcaggctgg gtcatcggcg ctagcggacg aaaaagccct
     3721 gaagggctaa gtcgttatgg ggtcgtcttc atcttccgaa tagcagggtg acaggtgccc
     3781 gctcattcac gtgggcagat ccttcccccg gaagatcttg cccgtggctt tggcacgatg
     3841 cctgctttat cgcactgtcg gaatgaaatt tatcgcattg ttggaaaaaa atgtctgtcg
     3901 gaaataggac cgaaacgact cgattgccgg ggtgaagtgc gcattatgcc gccccgacag
     3961 gccgacgtcc atgggaggga tcggattaag cggcacgatg attgtttcgg caccacgtcc
     4021 gagaggatcg tgacgcccag tccgttggca accatcggtc tgatggcctc acctgcggag
     4081 gtccgtagcg tcaccgacgg ctgatattgc ggcggagatc accggtcttg tcgatcagcc
     4141 ggcgtcacct gtcgttctgc cgcgtcgcgg cgacaaagcg atcgaggata tcgtcaagga
     4201 tcggcatcga cgttgcgaag ttgaacctga caaactgcgc gcaacccgga tcaaacgtct
     4261 cgccggcact aaatccgatc cgcgcgttgt tgagaaagaa ctggaacgcc ggggtcgaaa
     4321 gcccaagctt gctgcaatcc agccagccga gataggtcgc ctccggcgca tggaaacgga
     4381 tttccggcac ttccgccgcc agcacatcct tgacccggtt tcgcgccttg agcagatatt
     4441 ccattaccgc gtcaagccag ggctggcatt cgctccaggc cgcgaccgtg gcgtcgacgc
     4501 cgacgatatt ggctgaacct gtcagtctct gcgggatgcg tttgtgaaac cgatcgcgca
     4561 agtcttcggc gccgaaggcc atgagcgcgc aacgtaggcc gggaatattg aaactcttgg
     4621 tcgccgagtt gagggtaacg gtgcgggcgg cgatttccgg cccgagcgac gcaaagggaa
     4681 tgtgctgcag ccccgggaat accaggtcgg aatgtatctc atctgatatg acgacgagat
     4741 cgtgttcgat ggcgaattgg cccaatgcca gcaactcgtc acgggaaaac acccgccctg
     4801 tcggattttg cggattgcag agcacgaaga tacgggttcg cttgttgacc agcttttcaa
     4861 gctcgttcat gtcgaagacg aagcgggtcc cgtcgtcgcg catcggcagc gcaagaagct
     4921 cgcgctccgt cgagttgatg gcgtcgcgga acggcggata gttcggggtt tgcagaacga
     4981 cgccgtcgcc gggctcggag aatgccagga tcggcgcata ggtgccctgt acgagatccg
     5041 cgagcggcag cacaaggtca ggcgagagct cccatccgta gcgggatttc atccgctggg
     5101 cgaacgcacc ggcgaccagc cggtccggct tttcaccgtt gcgcaacgca tagccgtagt
     5161 cgcggtctcg gacgatccgc tcgatcgcgg actggatcgg ggcggcaacc gaaaaatcca
     5221 tgtcggcgac aaaggccggc aatatatccg catcgtactg gttccatttg gcattgcggc
     5281 gtgcctgcaa agcgggctgg gatagttcaa agctgatcgt ctcggtgctg gtcatatggt
     5341 ttcgcggggc tttcatgtga tttgctggga gaggtagccg cagattatgc cagatgccaa
     5401 gcgaccgcgc tgccatcctg ccgtgttagt cctttggctg ccttcgatcg tggcgggaat
     5461 aaagctgggg ctttttctgg tcatgatctc ggaaacggtt gcgccaatga tggcgtcggc
     5521 gtcataattc gaaaaacact tggcaggttc tgacggtcga caccatgcgg gtcggcctgg
     5581 gtatgtcggc ccgccggtca tcgcgcttct cggcttcgtc ttcccggtcg ttgccaatga
     5641 gttcgagcgg cggctcattc cctggaagtc cagtgcctga ctggaggcaa caatgtcgac
     5701 ggctttgcgg atcgtccacg gcaatttcgg cagggtcgcc ctgctcgata tggattgctc
     5761 gctggtccgc catgcgcacc cgcattgtca tgtcctgttc aaggtggcag gcgcggatac
     5821 ccaattcgtc gtcggcaacc gcattcatgc tttgaccgat tccgccgcgg tgctcgtcga
     5881 cggctggcag ccgcattcct acgttcacga cccggcgcgt ccacggacgc ttatccttgc
     5941 tctctacgtc gagcctgaat ggctgaaggc gtttcggccg ggatgggccg caagcggcgc
     6001 gcccggattt tttgaaactc cccatggcga ggtatcgccg ggcatccgaa cactcacgat
     6061 ggatctcgcg tccgagatga tggcggatcc cgaatgccag gccgaatacg aagcgttgtt
     6121 gtcggaactg atgatcgcgg tgatcgagcg cttcacgccc tggaagagct ttccgagatc
     6181 gatccgcgaa ttgaacgtgc cggcatgcga tcggcggatc cggcgcgcgg cggaatatat
     6241 ccgttccaat gccggcggcg aagccagcat cgatacgctg gcgaaagaag ctggtctttc
     6301 gcgcgcgcat ttcttcaggc tgttccagtc gtcgatcggc ttgcctccca aggtctatct
     6361 caacgtcgtg cgcatggaac gggcggtgga agccgtcctt catcgccaag ttcccgtcgg
     6421 cgagctcggc gcacgcctcg gattttccga tcctgcgcat ttcacccgct ttttccgcaa
     6481 taatgccggc gtttccccga gcgagttccg gcaagtatcg cgtctcgcgg gttgatgcgg
     6541 cgtggcaacc caaagaatga gacgaattgg caagttgcga gagcgcaggg tatggtcggc
     6601 gttctgcttt gctgaaaaca gtctccccac acgccgtcaa ggcgtgattg gcaggatgtt
     6661 cttcggatgc tgcatgcagc gggcccagca atagccgaga gcaaagcggc tcgcccttgg
     6721 gttggccaat cgattccgcg cgtcgaagac gccgcgttgc tgaccggcaa aggccgcttc
     6781 atcgacgacc tcggcgttcg gccgggaacg ctatatgccg cgattctgcg ctcgccgcac
     6841 gcgcatgcgg agatcctctc gatcgatggc gaggccgcgc ggcgcgcgga cggcgttgtc
     6901 gcagtcctga ccggcgacga catcaaggta ctgacaacca gcctcgtcgt tggcgtgaag
     6961 gcgaaggtcg aatgctggcc gatcgccgtc gggcgagtgc gctatgtggg cgaatcggtt
     7021 gccgtcgtgg tcgcctccga ccgatacaag gccgaggacg cggtcgacct gatcgacgtg
     7081 cagtaccgag cgcttcccgc caatatcgac ccgcttcagg cgctgtcgcc aagctcgccg
     7141 gtgattcatg acggcttcga cggcaacgtc gcgagcgacc ggactttccg ctatggcaat
     7201 cccgagcgcg catttgccaa agccgcgcac cggatttcga tcgacatccg ctacccccgt
     7261 aattcatgca cgccgatcga aacctatggc gtgatcgccg actacgacgc gtccgaggac
     7321 gccttcgata tcctcgccaa tttccagggc ccgttcagca tccacgcggt gatctcgcgg
     7381 gcgctgaaag tacccggcaa tcgcgtgcgg ctgcgcaccc cgccggattc cggcggcagt
     7441 tttggcgtca agcagggcgt gtttccctac atcgtgctgc ttgccgccgc atcgcgggcg
     7501 gtcggacgtc cggtcaagtg gatcgaggat cgtctcgagc acctcaccgc ctcggtatcc
     7561 gcgaccaacc gcgcgaccac gctttcggcc gccgtcgcgg ccgacggcag gattttggcc
     7621 ctcggctggg atcaggtcga agactgcggg gcgcatttgc gcgcgccgga gccggccacg
     7681 ctttaccgca tgcacggcaa tctgaccggc gcgtacgata tccccaacgt ggcggtgaga
     7741 aatcgggtcg tcgtcaccaa caagacgccc tccggcctga accgcgggtt tggcgggccg
     7801 caggtctatt tcgcgctgga gcggctgatg cagcgcatcg ccgtcgagct caagctcgat
     7861 ccattggacg tgatcagacg aaacctggtg ccggccgacc ggtttcccta ccggacggcg
     7921 accggcgcgc tcttggattc cggcgattac cgagaggcga ttgcgcgcgg cgtcaaggga
     7981 ggcgcgttgg acgaactgaa ggcgcgccgg gatgtcgcgc gggccgaagg caggctctac
     8041 ggcatcggct atgcggcggt ggtcgagccc agcgtttcga acatgggcta tatcaccacc
     8101 gtattgacgg cggccgagcg aaaaaaagcc gggccgaaaa acggggcgca ggccacggcc
     8161 accgtggccc tcgaccccgt cggcagcgtt tccgttcacg ttgcgtcggt gccgcaaggc
     8221 caggggcatc ggaccgtgct ggcgcaagtg gtggccgacg tcttcggcct cacgcccgcc
     8281 gacgtcaggg tcaataccga gatcgacact gccaaggacg cctggtcgat cgcgtcaggc
     8341 aattacgcaa gccgttttgc cgcggcggtc gccggaaccg ccaagctcgc ggccgaacgc
     8401 atcgcggccc gcctggcgcg cgtcgccgcg agccagttca ataccgatgc ggacgacatc
     8461 gtgttcgccg acggcgccgt gagttcgcgg cgaaacccgg ataacaggct ggcgttctcg
     8521 cgactggccg cgctgagcca ttggtcgccc ggcaccttgc ccgacgacgt ccagcagacg
     8581 atccgcgaga ccgtgttctg gacgtcgccc gagctcacgc cgccggacga cgaggaccgc
     8641 atcaacgcgt cgctctgcta cggatttatt ttcgacttct gcggtgtcga aatcgatcgc
     8701 accaccctaa tgacgcgtat cgaccgctat gtgacgatgc acgattgcgg caccatcctt
     8761 caccccggaa tggtcgacgg acaaatccgc ggcggattcg cgcaggccct gggtgcggcg
     8821 ctctatgaag aatacgccta cgccgcggac ggcacttttc tgaccggcac tttcgccgac
     8881 tatctgctgc cgaccacggt tgaagtcccg gatcccgtca tccttcacat ggagacgccg
     8941 tcgccgttca cgccgctcgg atcgaaaggc gtaggcgagg gcaactgcat gtcgacgccg
     9001 gtctgcatcg ccaatgccgt cgccgacgcg ctcggcgtca acgatgtcga gttgccgctg
     9061 gttccggcca agctggccga catcgtccgc ggcgccgaac cgccgccgcc cgccgggcgt
     9121 cgtgccgatg tgccgaaatc cgccaacggc ggccgcaagc tccgcggcga gggcagcgcg
     9181 acggtggccg catcgcccga ggcggtgtgg accatgctgc tcgatccgac aacgcttcaa
     9241 gcggtcatcc cgggttgcca cggcgtggag aaaatttccg acacgcattt tcgtgccgag
     9301 gtcacgattg gcatcgggcc ggtcaagggc cgctatcgcg cggaagtgac actgtccgat
     9361 ctcgatgcgc cgcgggcggt gaccctgggc ggcttcgccg aaggagcgct cgggctaggc
     9421 aagggcgaag gccgcatcac gctgacgccg gagcaggccg gcacggtggt gcgttacgaa
     9481 tatgacgcgg cgatcggggg caaggtcgcg agcatcggcg gccgtctctt ggacggtgcg
     9541 gcgcgcgtca tcatcggcca gttcttttca ggcctggccc gtcaggccgg cgggacctcg
     9601 gcgcaaggcc ggccgtcgct gctgggcaag cttttgtcgt tgttcgggag gcggccgtga
     9661 agcctgcgcc cttcgactat gtgcgcgcgg aaacccttga cgaggccctt gcggttctgg
     9721 cctcggaagg cagcgacgcg caaatcatgg cgggcggcca gtcgctgatg gcgatgttga
     9781 acacccgcct ggcgaagccc aaagtcctgg tcgacatcat gcggctgaag gaattggacc
     9841 ggatcgacgc gacgtctgcc gccgtgacgg tcggcgccgg cgtccggcag cggtcactgc
     9901 tgggctggcc gccgctcgct gaaaaactga agctggtcgc gcttgctctg ccctggaccg
     9961 ggcacgccca gacccgcagc cgcggaacga tttgcggttc gattgcgcac gccgatccca
    10021 gcgcggaaat gccgctggcg ctgctggcgc ttggcggcga ggtgcacctg cgaaccgcca
    10081 gcaagcgccg cagggtcgcg gccgaaaagt tcttcaccgg catgatgtcc accgcccgcg
    10141 ccgccgacga aatgatcgag gcggtctcgt ttccgtacct gggcgtcgcg cgctgcgcgt
    10201 tccgtgaggt cgcgcggcgt cacggcgact ttgccatcgt cgcctgcgcg gcggtcgcca
    10261 gcaggcaagg cgttcgcttc gccgtcggcg gtgttgcgga tatggccgcg gtgcgcgact
    10321 ggccgcgtct cgatggcacc gcgctcgacg acgccctgaa cgctttcgcc catgaactgg
    10381 atgcgcggga cgatgtccat gcgacggccc gttatcgccg cgatctggtc cggctgatcg
    10441 gtcgcgatct cgtccgcgag gtgctgcaat gacgcgcctt gagtcggacc ggcagcaccg
    10501 gatcagcttc accttgaacg gcgcggccgc ccaaggcgaa gccgagccgc gcatgctgct
    10561 gacggatttt ctgcgccacc agatcggcgc caccggcacg catgtcggat gcgagcacgg
    10621 cgtttgcggg gcctgcaccg tgaccgtgga cggaatgatc gcccgcgcct gcctcacgct
    10681 ggcggtgcag atcgatggca gcgacgtccg caccatagag ggcctcgcgc ccgctcccga
    10741 tcgattgggc atcctgcagg cggcattcaa gcgccatcac gcgctgcaat gcggcttctg
    10801 caccgcgggc attctgatgt cgctcgatca ctaccttcgt gaacggccaa aacccactga
    10861 agcggagttg cgcgatttcc tgagcggcca tctgtgccgg tgcaccggtt acaccccgat
    10921 catccgcgcc gcgctcgacg ccgctgactt gctggcaaac caacctcgtg cgaaaatgaa
    10981 gacagatgtt tgatctcggt accagtttcg tcgccagtgt ggcacgcgat ccaggcgccg
    11041 tcgcgatcgt ggacgccggg ctgcggctga cctacgcgca atggtttgcg cggatatctt
    11101 ctgtcgtcgc tgcattcgac gatttcggtt tgaggcccgg cgaccacatc gtcaccgtgc
    11161 tgcagaaccg atgggaggcc gcgaccattc actgggcctg ccagtttgcc ggattggtca
    11221 tcacgccgat caactggcgg gcgaaatccg acgagctcga tttctgcatc gagaattcgc
    11281 aagcccaggc tctcgtgtac gaaagcgttt ccgcgggcgc ggtctccggc gccaggttcg
    11341 cgcgaaattt gcgccgcatc gttgtcggcg acgccgtcgc agccgacgac caggccgtgc
    11401 tgtttgcaac catgatcgac acgcccgcgc cggaagccgt gccgcgtggc ggtccggagg
    11461 cgtggtcgat catgctctat acgtcgggca cgacgtcgaa gccgaaaggc gtgccccgcc
    11521 gtcaccgcgc cgagcgagcc gccggtgtcg ctcatgtggc gcaaaacctg tacggccgct
    11581 acgagcgcac ccttggcgtg atgccgctct atcacaccat gggcgtgcgc tccctgatct
    11641 cgatgtcgct gatcggcggc acgtttgtgt gtcttccccg ctacgatccc ggcctggcac
    11701 tggccatcat tgaggcggaa aaaatcacaa atctctatct ggttccaacg ctttaccacg
    11761 atctggtcaa tcacgcggac ttcgaaaaga cgaatacatc gagcatacgg aagctcggct
    11821 ttgccggtgc gccgatgaca gacggcctgc tgcagaagct gaattcggcg ttcaagccgg
    11881 atctgttcgt caatcattac ggtagctcgg aaatctacac cttcacggtc aatcaaaatg
    11941 cggtcgcgaa gcccggctca gccggcaaag ccgggatcaa ccagttcgtc agggtcgtca
    12001 agcttgccgc acgttcggca tcggagctgg cgtcgaccga agaggagggc gagatcatcg
    12061 cgctgctggc gagcgacgag tccttcgaag gctattggag gcgtcccgat gccgactcca
    12121 agtcgctgcg cgagggatgg tatttcaccg gcgataccgg cttcgtggac aaggatggcg
    12181 acctgttcgt caccggccgg accgacgaca tgatcatcac aggcggcgag aacgtatcgc
    12241 cggtggagat cgaaagctgc ctgtcgctgc accccctggt gtcggaggtc gcggtcgtcg
    12301 gtctcgccga cgagcgatgg ggcaagatcg tcgccgcgtt cgtcaagcgc agcgggtatg
    12361 tcagcgaggc cgatctcgaa gcattctgcc gggattccgg gttggcgaac ttcaagcgtc
    12421 cgcgaaaatt tgtcttcgtc gatgcgatcc cgaaatcgcc ggtaggaaag ctgctgcggc
    12481 ggctgctggt cgccggggaa tacgacgtcg agcgccggcc gccatcgacc gccgcatagg
    12541 tttcgatttc gaagaagaag gaagaaacaa atgccatcat cgttcaattt tgccgatcca
    12601 cggctgtcga agctcgacgg ctttcaggtg gaaatcgacg aaactcacga acgtgccgat
    12661 atcattctca accggccgcc ctacaacatc gtatcgatgc ctcagcgcga tcagcttcgg
    12721 ctggtgttcg aaaccctcga cgaggattcg cgggtccggg tcattgtggt gcgatcgatc
    12781 ggcgagcatt tctccagcgg cggcaatatc ggaggcttca tggaagccac gcccgagcac
    12841 gtgtcgaagc ttgcctggaa tatcgcctcg cccgcgcgct gcgccaagcc ggtgattgtc
    12901 gccaatcgcg gctattgctt cggcgtcggg ttcgaactgt ctttggcctg cgactttcgg
    12961 atcgcgtcgg agacgacatt ctatgcattg ccggagcaga agctcgggca gattccgggc
    13021 tcgggcgggt ccgcacggct gcagaagatg atcggcatca cccgcaccaa ggacatcgtg
    13081 atgcgttcga agcggattgc cggccaacag gcccaggaat ggggcatcgc caccgagtgc
    13141 gtgcccgatg ccgaattgga gaaggcgacc gacaagcttg tcgacgaact ccgcacgttc
    13201 tcgccgctgg cccagcgaac cgccaagaag ctcctgaacg acaccgagga ctcgacgctg
    13261 gcgatcgcga tcgagctcga gggccattgt tacagccgcc tgcgtcaatc gtcggacttc
    13321 aaggaaggcg tcgaggcctt caacgccaag cgcgctccga actttaccgg gctataaccg
    13381 atgccggatg gggcccggca cacacgatac cgggtcggtc cggatggtcc ggcggcccgg
    13441 tcgcaatgcg actgggtttg cgaaaatcac aaaagagggg cttcgaaatg acaacgacaa
    13501 gtgcgccgct ggtggatgcg caggcaccgg ccgacaaccg gcaaatgatc aatgcgatca
    13561 tcgcctcact gctgggctgg tcgctcgatc tgttcgatct gtttattctg ctctatgtcg
    13621 cgccggtcat tgggactctg tttttcccct ccaccaatgc gacgctgtcg ctcgcagcgg
    13681 tctatgcctc gtttgcggtg acgctgctga tgcgccccat cggctccgcg atattcggcc
    13741 atttcgccga tgtccatggt cgaaagggcg ccatgctggt cgcgatcgtc ggcgtcggcg
    13801 tcagcaccgc ggcgttcggt gcgttgccga cgatcgcgaa ggtcggcatc gccgcgccca
    13861 tcatgttcct gatcctccgt ctggttcagg gcatattcgt cggcggtgtt gttgcgtcga
    13921 cgcataccat tggaacggaa tcggttcccg cgaactggcg cggggcaatg tcgggcctgg
    13981 tgggcggcgg tggcgcgggc atcggtgcgt tgctggcgtc attcgtattc ctgatcgcat
    14041 cgtcggtatt cccgggtgag gcctttgcgc aatggggatg gaggttcatg ttcttctcgg
    14101 gccttctgag ttcgttgctc ggatggttca ttttcaagaa cctcgaggag tcgccgttct
    14161 tcaacgagtt gcagcaacag aaggcggcgc gaaaggcgat ggcggcgccc tcgccggtca
    14221 gggtgctgtt ttcgagcggt taccgctccg tgctgctgat caacctcttg attacattcg
    14281 gcggcggcgc gggctactac ctgacgtcgg gctatctccc gagcttcttg aaggtcgtca
    14341 acggcgttcc gaacaacgtc tcgtccctga tcctgatagg cgcgagcgtg gtggcgttca
    14401 tctccgccgt cctgttcggg gcgttgagtg aaatgatcgg gcgcaagccg acctttctta
    14461 tcgtcggcgt ctgtgcgctg gtgtggttgc cgctgtgcta tctcggcctg gccaaggcaa
    14521 ccgatatcaa ggaagtgacg atctatgccc tggccatcgc attcatcggc aatgccggct
    14581 atgcccccgt cctggtattt ctgaacgaga ggtttccgac ctcgttgcgt gcaagcggta
    14641 cgggattatc ctggaacatc ggcttcgccc tgggtggcat gatgccgacc ttcgtctcgc
    14701 tcgcaagcgc cggcacggcg cagatcccga cgtcgcttgc gatattttcg gtggcgatct
    14761 acctgatcta cctgatcggc gcactgatca ttccggaaac caggggcaat tttcgttgac
    14821 ttggtccgcg tgttgaagcg cgaggcgcca ggattccaag atgccggcgc gagacaaaca
    14881 cctacgaaaa gacccgcgct gtccaaaaaa cggacagtta atgcgacccc tgacaatgcg
    14941 accctgacgc ccctaatgga tgggactcat ttcaggctag gtcgtacggg cagagatccc
    15001 ctcgacgagg gctgccggcc agaatttggc gggtaatccc gaggccgtgt ccacaagcaa
    15061 gatttggctt gccctctaac cgcccgaatc actctggagc ttccggcagg cccatgggtc
    15121 tgccggattg aaccatgatt aggacagtgt ggctcacgat tgcgtgtcta gccgtgctcg
    15181 ccgccttgct ggtcggcaag gctctcaaaa cacctgtcgc tctggccgcg gtgacaccga
    15241 tcgacgagac gacaatcggc atcggttcag ctcaagatcc attgaccaag gctgatcggc
    15301 ttgaatttgc cggtgtgcgt ccggaaatcc cggcccagtt cgcattgcgc ccgaccgtgc
    15361 cattgactcc gcccgctcca acaagcgtct cgccggtcga gaccaagatc gtcagccggg
    15421 attggcacga tccaaacgct acggcttcat tggccggaaa gtccaagcag cctgcgcgaa
    15481 tggcgactgg caagaaagcc aaatccgtgg atgccaaggg gaggcgaacg gcggatcgtt
    15541 ccaagccagc tgggcatgga aagccctgtc agcgaaccgg cgctttgggt gatttcctga
    15601 agtcattgaa gctgtcgccg gcctgcgtct catagctttt ccgcattcgt cacaggtggc
    15661 ctacgctttt cggatctctt ggctcgacct cgcacacctc tggctatttt ccgctggcgc
    15721 tgcgaccgcg cggcgggaca acgtttcggc tacgcgagca aaagcaacac cagcccagtg
    15781 ccggcgagcg ctccggtcca gagcaggtcg gggttgagcc atgcggtgcg caatattgaa
    15841 aggccaaccc attcgtagac aatgaccgcg accgccccgg tgactaccag catcgccgcc
    15901 gtgtggaccg caacggcggc aagcgcagtt gcggcgggcc cgcttgcccc tgtctcgccc
    15961 gctgatgagg atgcgaggca gagtgggatc atcgctggca ggatcatgaa tcccgcacca
    16021 tgcgctgtcg ccatgaggaa agaccaaaga atcagcccag cctgccccgt cttcataccg
    16081 acgcgtacac ggtgccggtg gccgtaaagt tggtggtaaa cggaccatcc gatcagcaga
    16141 aggccggccg agacttgtat cgcgcgttgg ttcacgagaa cgcctgccgc gacgaaggct
    16201 ccggccacca tcgcgatcga aactgcgtgg ccgaaggcaa ttggcggcag cgccaacaga
    16261 acagtggctc ggctccgtcg atgcagtccg agcgccacgg cgaacagcca gcccatggcc
    16321 gggtttagcc catggaaggc gccgagcccg gtcaaagcca gccagggcca ggcgccggtc
    16381 attgcgcgtg cccctcctta gcggacaggc cggcggccga acgcgcgatc acggatagca
    16441 atagctgtcg gacgagcagt cgccgccctc gaggcgaacc tgatgcgggc gatgtgattt
    16501 cggccattca acgaagaact tctcgtcgaa gctgatgccg ccctctggct tggcgtcgag
    16561 cttcaccatc cagccgtcga gtccatcggg atacaattgc gagtcgatgg tgccgtagag
    16621 cgagttggtg aaatacaccc gcttgccgtc gcggctgatc tcgaccattt gcggtccgcc
    16681 gctgagcgcc ccgttcttgg cacccggatg gctcgcgcgc gagacgacgc cgccgatgcg
    16741 caccttgccg gtgagcttgg gcttgaacgg atcggacacg tcgtattgct gcaggtcgcc
    16801 ggtgccccag caggcgacgt agaggaagcg gtcgtccatc gacagatcga tgtcggtgac
    16861 gaacggcgga accgccttga agcccttgag aatgggcggc agcttgtcgg gatcggccgg
    16921 ctcggccgga atgtcgatga tcttcgtgac cgcccacttg tcgccgtcgc gatgccaggt
    16981 ccagatcgag gacgacaggt ccttgaggct gatgacgcag ttgacgaagc cgtaggcctt
    17041 ggtgggatcg tgcgccgggc gcagctcgaa cacgagctgg tactccgggc cgaagtcgat
    17101 ctcctgcagg tgcttgcgct tggtgaaatc ccagaaatgc aggcgccggc cgtatttgcc
    17161 gccgagcagg acttccggca cgagcccgtt ctcgaaggtg tcgggcgtgc cccattcgct
    17221 cgtcaccagg gtgtcgtagc cgagatgcca ccagccatcg tagctcagcc gctgcgggcc
    17281 acggtcgacc tcccagcggc cgagcggctc gaagctctca tgatcgatca tgaagatgcc
    17341 gcccggcgcc ttgccgtccc tgttgccgag cgcagcgaca tagatgccgc cgggcccgca
    17401 atgcaccgtg tgcggccgcg tgtagccggt cttttccgcg atctcttcgg gctcgatgac
    17461 tttgacgatc ttcggcttgc gcgggtcggg cttggtgtcg aggatgtgga tgcgcgaaga
    17521 gcgcaagccc ggcaccacca gatagcgccg ctccacatgc ggatgcggcg cgttcgggca
    17581 caggcacgag gagcaggcgt tccagccgaa atgatgcagc tcgtcgccca cgctcggcat
    17641 ttccacgttg ccgaggattt tcgcatagct tttggagccg ggatcgacgt cgacgaccgc
    17701 gatcgcgtcc ggcttcttgc gcgtcggatc gaacgcggcc acataggcga acttctccgg
    17761 cggcgccttc atggccatgc gcggggatgg ataaaagctc ggatcgggtc tccaggtcat
    17821 ggcggcctcc catcggcttc gcaaattcgc tggggttaag cttagcgggt tccggtggcg
    17881 ctggcgatgg accccggcac gggattctcg tgcaagcgat ttcggcaaac gccagg
//
LOCUS       DATNOI010000092         2224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2626366,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000092 DATNOI010000000
VERSION     DATNOI010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2626366"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VN968_03215"
     CDS             <1..442
                     /locus_tag="VN968_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HXL28292.1"
                     /translation="GKNANWLWAWSLAIPAGSKKVAAAEKFIAWATGKDYIKLVASKE
                     GWANVPPGTRTSLYQNPEYLKVAPFAKLTLASIDAADPNKPTVQPVPYVGVQYAAIPE
                     FQGIGTTVGQQFSAALSGSSTVDAALAAAQSATEREMKRAGYIK"
     gene            560..1432
                     /locus_tag="VN968_03220"
     CDS             560..1432
                     /locus_tag="VN968_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL28293.1"
                     /translation="MATQQTQVLGRALLTPAVALLFIWMIVPLAMTIYFSTLHYSLLD
                     PGSESFVGLENFRYFLTDPAFLTSLQNTLVLVGSVLAITILVGTPLALLLDQPVIGRS
                     IVRLMVIAPFFVMPTVSALVWKNLLMHPVSGLFAWGATLLGLTPIDWFTDAPLFAVIL
                     IVAWEWLPFATLILLTALQSQDEEQKEAAEMDGASALSTFIYLTLPHLARPITVVILI
                     ETIFLLTVFAEIFVTTGGGPGLQTTNIAFLIYSQALIQFDVGNASAGGLVAVVIANIV
                     AFFLVRIIGRNLEA"
     gene            1436..>2224
                     /locus_tag="VN968_03225"
     CDS             1436..>2224
                     /locus_tag="VN968_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL28294.1"
                     /translation="MARKVTTRRVWVSTAAAWLFGFLIFLPILWMVLTSFKTELEAFA
                     MPPTFLFFHWTAENYATVQGRSDYLHHALNSIMVAGGSTLIALLIAIPAAWSMAFAPT
                     KRTKDVLLWMLSTKMMPPVGVLVPIYLIYRDFGLLDTRGGLILILCLGNLPIVIWMLF
                     TYFKEIPKDILEAARMDGATIGRELIYVLTPMAVPGLASTLLLNFILAWNEAFWTLNL
                     STLDAAPLTVFIASYSSPEGLFWAKLSAASTLAIAPIIILGWFTQ"
BASE COUNT          384 a          681 c          662 g          497 t
ORIGIN      
        1 gggaaagaac gccaactggc tgtgggcgtg gagtctcgcg attcccgcag gttcgaagaa
       61 ggtcgccgct gcggaaaaat tcatcgcatg ggcaaccggc aaggactaca tcaagctcgt
      121 cgcttcgaaa gaaggatggg cgaacgttcc gcccggcaca cggacttcgc tgtatcagaa
      181 tcctgaatat ttgaaggtcg ctccgttcgc gaagctgacg ctggcttcga tcgacgctgc
      241 cgatccgaac aagccgacgg tgcagccggt gccttacgtt ggggtgcaat acgcggcgat
      301 cccggaattc cagggcatcg gcaccaccgt cggccagcaa ttttcggctg cactatccgg
      361 ctcatcgacc gtcgacgcgg cgctcgccgc cgcgcaatcc gccacagaac gcgaaatgaa
      421 gcgcgccggc tacatcaagt agctggcgtc gatccttgtg cggcgattct ccctatcagc
      481 cgcaaactgg ctgccgtccg cccttcatag ttcacggacg gcagccctca tcgagtttcc
      541 ctgaataaag gaggcggcgg tggcgaccca gcagacacag gttctcggac gggccctgct
      601 gactccggct gtggcattgc tgttcatctg gatgatcgtc ccgctggcga tgacgatcta
      661 cttttcaacg cttcactaca gcctgctgga tccgggtagc gagagcttcg tcgggctaga
      721 gaattttcga tattttctga ccgaccccgc gtttctgacc tcgctgcaga acaccctggt
      781 gctggtcggt tcggtgctcg ccatcaccat tcttgtcggt accccgctgg cgctgctgct
      841 cgaccagccc gtgatcgggc gcagcatcgt ccggctgatg gtgatcgcgc cgtttttcgt
      901 gatgccgacc gtcagcgcgc tggtctggaa aaacctgctg atgcatccgg tttccggatt
      961 gtttgcctgg ggcgcaacgc tgttgggctt gaccccgatc gattggttca ccgacgcgcc
     1021 gttgttcgcg gtgatcctga tcgtcgcctg ggaatggctg ccctttgcga ccctcatcct
     1081 gctcacggcg ctgcagtcgc aggacgaaga gcagaaggag gcggccgaga tggacggcgc
     1141 gagcgcgctt tcgacgttca tctatctcac tctgccgcac ctggcgcggc cgatcacggt
     1201 ggtcatcctg atcgaaacga ttttcctgct gaccgtgttc gccgagattt ttgtgacgac
     1261 cggcggcggc ccgggcctgc agaccaccaa tatcgcgttc ctgatctact cgcaggcgct
     1321 gatccagttc gacgtcggaa acgcctccgc aggcggactg gttgcggtcg tgatcgccaa
     1381 tatcgtggcc ttcttcctgg tgcggatcat cggacggaat ctggaggcgt aatcgatggc
     1441 acgcaaggta actacgagac gcgtctgggt atccacggct gcggcctggt tgttcggatt
     1501 tttgatcttc ctgccgatcc tgtggatggt gctgacgagc ttcaagaccg agctcgaggc
     1561 attcgccatg ccgccgacgt tcctgttttt ccattggacc gcggagaact atgcgacggt
     1621 gcagggccgc agcgactacc tgcaccatgc gctcaattca atcatggttg cgggcggatc
     1681 gacgctgatc gccttgctca tcgctattcc agcggcctgg tcgatggcgt ttgcgccgac
     1741 caaacggacc aaggacgttc tgctctggat gctctccacc aagatgatgc cgccggtcgg
     1801 cgtactggtt ccgatctatt tgatctatcg ggattttggc ttgctggaca cgcgcggcgg
     1861 cctgatcctg atcctctgtc tcggcaatct gccgatcgtg atctggatgc tgttcaccta
     1921 tttcaaggaa atcccgaagg acatcctcga agcggcgcgg atggatggcg cgaccatcgg
     1981 ccgcgagctc atctatgtgc tgacgccgat ggccgtaccc ggccttgcct cgacgctgct
     2041 tctcaatttc atcctggcgt ggaacgaggc gttctggacc cttaaccttt cgacgctgga
     2101 cgcggcgccg ctcacggtgt tcatcgcgtc ctattcaagt ccggagggcc tgttctgggc
     2161 gaaactgtcg gcggcctcga cgctcgccat tgctccgatc atcatactcg gatggttcac
     2221 ccag
//
LOCUS       DATNOI010000093         2578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1948611,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000093 DATNOI010000000
VERSION     DATNOI010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1948611"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1579)
                     /locus_tag="VN968_03230"
     CDS             complement(152..1579)
                     /locus_tag="VN968_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXL28295.1"
                     /translation="MNIVQPSASPLSPELIARFRKIVGNKYAVTDAADIAPYVTEERD
                     LFHGRSPLVLRPGSTAEVSAICKLASEQRIALVPQGGNTGLVGGQTPHHGEVVISMRR
                     MDKIRDIDTASNTMTCEAGVVLQVAQQHASELDRLFPLSLGAEGSCTIGGNLSTNAGG
                     TTALAYGVAREMALGLEVVLADGRILNGLSKLKKDNTGYDLRNLFIGAEGTLGIITAA
                     TLKLFPKPHAVETAFVGLRSPAAALKLLSISQNEAAGTLTSFELLSDIAVDFSVRHGI
                     DIRDPLSNKHPWYVLMELSSPRDDARAALESILAQGMEQAIVDDAVIAANLSQRMAFW
                     KLRDEMSAAQKPEGGSIKHDISVPVAAVPDFIEQANAAVIKLIPGARPVPFGHLGDGN
                     IHYNVSQPVGGDPADFLARWHEVNEVVFDIVLRMGGSISAEHGIGVLKRDELPQVKDK
                     VAIELMRSIKAMLDPLGIMNPGKVL"
     gene            complement(1600..2578)
                     /locus_tag="VN968_03235"
                     /pseudo
     CDS             complement(1600..2578)
                     /locus_tag="VN968_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474709.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
BASE COUNT          458 a          845 c          813 g          462 t
ORIGIN      
        1 aaaggcgcgc atgagaaatt ccctgccgtc attgcgagga gcaatgcgac gaagcgatcc
       61 agctttccct cgctctggat tgcttcgctg agcctgtcat cgggcgcgca ttcgcgcgac
      121 ccgttggctc gcaatgacga gagcccgttc gctacagcac cttgcccggg ttcatgatgc
      181 cgagcggatc gagcatcgcc ttgatggaac gcatcagctc gatcgcgacc ttgtctttca
      241 cttgaggcaa ttcatcgcgc ttgagcacgc cgatgccatg ctcggcggag atcgatccgc
      301 ccatccgcag cacgatgtcg aataccacct cattgacctc gtgccagcgc gcaagaaaat
      361 ccgcagggtc gccgccaacc ggctggctga cattataatg gatattgccg tcgccgagat
      421 ggccgaacgg caccggccgc gcgccgggta tcaactttat caccgccgca ttggcttgtt
      481 cgatgaaatc aggcaccgcg gcgaccggca ccgaaatgtc gtgcttgatc gagccgcctt
      541 ccggcttttg cgccgcggac atttcatcgc gcagcttcca gaacgccata cgctggctga
      601 ggtttgccgc aatcacggcg tcatcgacga tggcctgctc catgccctgc gccaggatcg
      661 attccagcgc ggcgcgggcg tcatcgcgcg gggaagacag ctccatcagc acgtaccatg
      721 gatgcttgtt gcttaaggga tcgcggatat cgatgccatg tcgcacgctg aaatcaaccg
      781 cgatatccga aagcagctcg aaactggtaa gcgtgcctgc ggcctcgttc tgcgagatcg
      841 agagcagctt cagggctgcc gccggcgatc tgaggccgac aaaggcggtt tccaccgcgt
      901 gcggtttcgg aaacaatttt agggtcgcgg cggtgatgat gccgagcgtg ccctcggcgc
      961 cgatgaagag gttgcgcaga tcgtagccgg tattgtcctt cttcagcttc gacagcccgt
     1021 tcagaatgcg cccgtcggcc agcaccactt cgagcccgag cgccatctcg cgcgccactc
     1081 cataggccag cgcggtagtg ccaccggcat tggtggagag attgccgccg atggtgcagc
     1141 ttccctccgc gccgagcgac agcggaaaca gccggtcaag ctcgctggcg tgctgctgcg
     1201 cgacttgcag caccacgccg gcctcgcagg tcatggtgtt ggatgcggtg tcgatgtcgc
     1261 ggatcttgtc catccgccgc atcgagatga cgacctcgcc atgatgcggc gtctggccgc
     1321 cgacaagacc ggtattgccg ccctgcggca ccagcgcgat ccgctgttcg ctggcaagct
     1381 tgcagatcgc ggatacttcc gcggtcgagc ctggccgcag caccagcggc gagcggccgt
     1441 gaaacaggtc gcgctcctcg gtgacatacg gtgcgatgtc agccgcgtcg gtcaccgcat
     1501 atttattgcc gacgatcttg cggaaacgcg cgatcaattc gggcgagagc ggcgacgctg
     1561 atggctggac gatattcatt cttgtcactt tacgcttgct catttccgcc ccatggcggc
     1621 gcggcgcagc cgatcgttga tcgcttcgcc gagcccgtga tctggcaccg gcatgacagc
     1681 gatggcgcgc gcgccatcgg cgtcgagcgc gcgaagataa ccgaaaagat tggcagcggc
     1741 ctcgttaaga tcgctatgtg cggacaaatt catcaccgcg gttgcgctat ctgctccctg
     1801 caccgcatca gggccgaacg ccagcagcgc ttcaccggcc tcgatgctct ccgcattgag
     1861 ccgcaccggc gtgcgcggcg cgtaatgcga ggccagcatg cccggcgcca ggggctggct
     1921 tgtattgttg tcgccatctt cgggcggttg caccagcgca cggccgagca cgcgttcgat
     1981 ctcgccgcgc ggcacgccgc cgggccgcag cagcatcggt tcgtcaaaac atccgacaat
     2041 ggtggattcg acgccgaccg cgaccgcgcc gccgtcgacg atcagatcta tgcgcccggc
     2101 gagatcgctt tgcacatgcg ccgccgtcgt cggcgagacg tggcctgaca gattggccga
     2161 tggcgccacc accggtccgc cgaatgcgcg caggatggcc cgcgctagcg gatgggcggg
     2221 cacccggatc gcgacgctat cgagacctgc ggttgcgaga tcggcaaccg cgcagtcatt
     2281 tgtcttcggc agcaccagcg tcagcgggcc cggccagaat gcttctgcca gcgcgatcgc
     2341 ccgcgcctcg aagcgggcaa caagccgcgc agccgcgagg tctgacatgg gcaatcagcg
     2401 gattgaacga gggccggccc ttggcctgat agagccccgc aatcgccgca gcattggtgg
     2461 catcggcgcc gaggccatag acggtctcgg tcgggaacgc gaccagcccg cccgccgcga
     2521 gagaacgcgc agccgcggcc acggcggcct cgccggcggg cagaatctgt gttttcag
//
LOCUS       DATNOI010000094         2653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1727886,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000094 DATNOI010000000
VERSION     DATNOI010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727886"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..693
                     /locus_tag="VN968_03240"
                     /pseudo
     CDS             1..693
                     /locus_tag="VN968_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509296.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alkene reductase"
     gene            799..1659
                     /locus_tag="VN968_03245"
     CDS             799..1659
                     /locus_tag="VN968_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004129492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HXL28296.1"
                     /translation="MKIGVSGASGHLGRAVVSELLQRAGGREVVAITRTPETVSGSAH
                     ARFGDYDRPESLAEAYAGLDRLLIITTVAPEPGKRGAQNVAAIDAAVKAGVKHIVFMS
                     AVGTRKEEEPARGASYWRGEQHLIATAPAWTILRMNFYAEAFLQLAQAALNQGVLAGL
                     AENRAAFVARDDVAAAAAGILIGDGHAGAIYNATGPERLSGAKRAGVIAEITGRPLAF
                     RVIMEQQLRAGLTQAGLPAGAINIVVGIQASFAAGVFDIVTGDVERLGGRPPKPLRDV
                     LAAALKSPSA"
     gene            1680..2045
                     /locus_tag="VN968_03250"
     CDS             1680..2045
                     /locus_tag="VN968_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXL28297.1"
                     /translation="MPQTASTLLLRNLHEVFGEIDPVRRRAAIDEIFHEDAVFYEPNG
                     AYRGRDEIDRIAGVIKATHPDFQYQPLSPPEEIGDGCRVRWVSGSPGRPPAYAGTDFI
                     IVRDGKIAAVYLFFDKLPN"
     gene            2321..>2653
                     /locus_tag="VN968_03255"
     CDS             2321..>2653
                     /locus_tag="VN968_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXL28298.1"
                     /translation="METNMSAVSIQSSRPSRRIGAWTLQAIIAAAFLAAGAAKLAGVP
                     FMVDLFAQIGIGQWFRVVTGVVEVVGAVALLFPGLASIGALWLGGTMVGAVATHLFVL
                     HTSPVPAIV"
BASE COUNT          463 a          857 c          842 g          491 t
ORIGIN      
        1 tcagatgttc accgcgacag ggatgctgga cattccgacg ccgcgtgcgc tgacgaccga
       61 ggaagttcgc cggaccgtcg ccgacttccg ccatgcggcg cgcagcgcca ttgaggccgg
      121 cgccgacggt gtcgagattc acggcgccaa tggctatctg gtccagcagt tcttcgcccc
      181 gagcgccaac acgcgcgccg atgaatatgg cggctcgatc acgaaccgcg cccgtttcgc
      241 catcgaagtc gccaccgcga ttgccgggga gatcggcgcg gacaggaccg ccatccgcct
      301 gtcccccggc atcaagatgt ggggcatcga cgaaggcgcg gaaggcccgg ccctctaccg
      361 ctatctggtc gccgaactcg acaagctcgg tcttgcctac ctgcacgtca tgcatgccgg
      421 cgacgagaaa ctggtggcga cgtccgcgcg ctgtggaagc agtcgctgat cgtgaaccgg
      481 cccggtcgtc cgcgcgaccg gatcggtgcc gacgtcgctt cggggttggc cgatctggaa
      541 gcctacggcc agctggtcct tgccaatccg gattttgtcg cacgggtgaa gaccggtgcg
      601 gcgatgaacg aagcggatcg cgccagcttc tttggtggcg ccgcgcaggg ctacaccgac
      661 tatcccgcgc ttgaggtggc cacggccgca tagggcgcgt ggcttgcccg caaacgcggt
      721 cagagcaagt gcaagcgcgg caaaggcgca gctcgcgctg cgtgcctgct ttgctctgtt
      781 atcccagtgg aggttgacat gaaaatcggt gtgagtggcg ccagcggcca tttgggtaga
      841 gccgtcgttt cggaattgct gcagcgtgcc ggcgggcgcg aggtcgtagc tatcacgcgc
      901 acccctgaaa ccgtctccgg atcggctcac gcacgattcg gagactacga ccggcccgaa
      961 agccttgcgg aggcttatgc gggtctcgat cgtctgctca tcatcacgac cgtcgctccg
     1021 gaacctggaa agcgcggggc gcagaacgtc gctgccatcg acgcagccgt gaaggcgggc
     1081 gtcaagcata tcgtcttcat gtcggccgtg ggcacgcgga aggaggagga gccggcgcgc
     1141 ggcgcctcct actggcgggg cgagcagcat ctgatcgcca cggcgccggc ctggaccatc
     1201 ctgcggatga acttctatgc cgaagctttt ctgcagctgg cgcaggcggc gctgaaccag
     1261 ggcgtgctgg cagggctcgc cgaaaaccgg gctgctttcg tggcgcgtga tgacgtggcg
     1321 gccgccgcag ccggaatcct gatcggcgac ggccatgccg gcgcgatcta caacgccacg
     1381 gggcccgagc gcctgtcggg tgcgaaacgc gcgggcgtca tcgccgaaat cactggccgg
     1441 ccgctcgcat tccgcgtcat catggaacag caattgcgcg ccggactgac gcaggcgggc
     1501 ctgccggcgg gggccatcaa catcgtcgtc ggcatccagg cgagcttcgc ggcgggggtg
     1561 ttcgacattg tgaccggcga cgtcgagcgg cttggcggcc ggccgcccaa accgctccgc
     1621 gacgtgcttg ctgcggcgct gaaatctccc tcggcctgaa gactaaccgg agaaagacca
     1681 tgccccagac agcatcgacc ttattgcttc gcaatctcca cgaggtgttc ggtgagatcg
     1741 accccgtgcg tcggcgcgca gcgatcgatg agatcttcca cgaagacgct gtgttctacg
     1801 aacctaacgg tgcttaccgt ggccgtgacg agatcgaccg catcgcaggc gtgatcaagg
     1861 ctactcatcc tgacttccaa tatcagccgc tctccccgcc tgaggagata ggcgatggct
     1921 gtcgggtccg gtgggtatcg ggcagccctg gcaggccacc agcttacgcc ggaaccgatt
     1981 tcataatcgt ccgggacgga aaaattgctg ccgtctatct cttctttgac aagctcccaa
     2041 actgaacgtc gcggtggcga cattgctcgc gccgccgcaa cccatactga caagccatca
     2101 tgaccaccca aggcggatta cgtccgagaa agcgtgagcg ctttgctaca tcgcgcctct
     2161 ggccgacctc tcgatcacag ccgtgcggaa aacatccatc acttcaggtc gcgcagcatc
     2221 ggtcgtgcat cggttaaacc gatggataat atctaaataa atcggctgtt gcgatgacgg
     2281 tcggcgtctt agcttcttct cactttcaac ctcacaccca atggagacca acatgagtgc
     2341 agtttctatc caatcatccc gtccttcccg tcgtatcggc gcctggacgt tgcaggcgat
     2401 catcgcggca gctttccttg ccgccggcgc cgccaaactg gccggtgttc ccttcatggt
     2461 cgacttattc gcgcagatcg gaatcggtca gtggttccgc gtcgtgactg gggtcgtgga
     2521 agtggtcggc gccgtcgcgc tgctctttcc tggcctggct tcgatcggcg ccctctggct
     2581 cggcggcacc atggtcggcg ccgtggcaac ccatctgttc gtcctgcaca ccagtccggt
     2641 cccggcgatc gtg
//
LOCUS       DATNOI010000095         9460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_511435,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000095 DATNOI010000000
VERSION     DATNOI010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9460
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511435"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="VN968_03260"
     CDS             complement(<1..1373)
                     /locus_tag="VN968_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL28299.1"
                     /translation="MLAKYSIRTKITAVVAFLLVAMSLMGALNVRQMYAINSSTVDIT
                     TNWLPSIRVLGELRAATITYRAIVRSHLLAAEEAGKQLQEDLLAKWINNTDKARRAYE
                     PLITTAEERALYDEFSTSWAEYLAGVKEVLALSRKNEDKEAKLLHAKASLAGVHADET
                     LQKDVLLNNKGADAAGSQAAESFDDAIKMVLASLLVAIILGIGAAILLVRDVSAGIKS
                     IVAPMQALGNGDLTAQVPHQGETTEIGSMANTLQVFKDALVAKKASDEAAAIDADAKI
                     RRGQRVDNITREFETLIGELVDSLSSASTQLEASAGNLTSTAERAQKLTTVVAAASEE
                     ASTNVRSVASATEEMASSINEIGRQVQESARMATDAVDQARKTNDRVSELSKAAARIG
                     DVVELINTIAGQTNLLALNATIEAARAGDAGRGFAVVASEVKALAEQTAKATGEIGQQ
                     ITGIQAAT"
     gene            complement(1550..2827)
                     /gene="otnK"
                     /locus_tag="VN968_03265"
     CDS             complement(1550..2827)
                     /gene="otnK"
                     /locus_tag="VN968_03265"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HXL28300.1"
                     /translation="MTLKLGCIADDYTGASDLANTLTRCGLRTVQTIGVPSADLALPE
                     VDAVVVSLKSRSIDAGLAVTGSRAAEKWLRKRGAAHVLFKICSTFDSTDAGNIGPVMD
                     ALRADSGDPIVLVTPAFPETGRTVYQGNLFVGALPLNESPLKDHPLNPMHDSNLVRVL
                     ARQSKTKVGLVDLAVIARGPDAVRAQLVDLSTRGFGAAVADAVFARDLETIGVVALDH
                     RLSVGASGIGLGLARALAASGPVTSNAPDAMADTAVGGPAACLAGSCSQATLQQIANA
                     ERVMPVFHLDSEQAVAGKAEAQRALGWANEHLGKGPVLIASSATPDQVAALQARHGRD
                     AAGHAIEQAMADIAQGLVQSGVRRLVVAGGETSGAVVDRLNIPGFLVGVEIAAGVPVL
                     RAVGANAGAMLLALKSGNFGGPEFFTDALKLMR"
     gene            complement(2824..3606)
                     /gene="otnI"
                     /locus_tag="VN968_03270"
     CDS             complement(2824..3606)
                     /gene="otnI"
                     /locus_tag="VN968_03270"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HXL28301.1"
                     /translation="MPRFAANLSMMFTEVPFLDRFEAAARAGFTAVEFLFPYDHPAEA
                     VGERLNRNGLTQALFNLPPGNWDAGEKGFAALPERFDDLQQSLRTALPYAQATGVKRL
                     HLMAGLADRGNRQAVQAFTKSVAWAAEFFAPHGLDVVIEPINSRNVPGYFLNDFSFAR
                     DLIAELKIPNLKLQFDIYHCQIIHGDVTMRLRAMMPIIGHIQIASIPSRHEPDDEELN
                     YPFLFEELDRLDYGGFVGCEYNPRGMTTDGLGWFKSYAGVKP"
     gene            complement(3679..4599)
                     /gene="ltnD"
                     /locus_tag="VN968_03275"
     CDS             complement(3679..4599)
                     /gene="ltnD"
                     /locus_tag="VN968_03275"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HXL28302.1"
                     /translation="MPESISAKPRVAVIGLGSMGFGMATSLRRAGFDVTGCDVSADAV
                     ARFVADGGGGANAPAEAAKAADIVVSVVVNAAQTETILFGKDGVAETLARDAVFVSSA
                     TMDPDVARRLAKQLEASGRHYLDAPISGGAQRAAQGELTILASGSAAAFAKARPALDA
                     MAAKLYELGDAAGLGAAFKMINQLLAGVHIAAASEAITFAAKQGLDIRKVYEVITASA
                     GNSWMFENRIPHVLDGDYSPRSAVEIFVKDLGIIQDMARTARFPVPVAAAALQMFLMT
                     AASGMGRDDDASVARTYARVTGTHLPGEPK"
     gene            4716..5375
                     /locus_tag="VN968_03280"
     CDS             4716..5375
                     /locus_tag="VN968_03280"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HXL28303.1"
                     /translation="MTESNIREEICRLGRSLFERGLTPGSSGNISVGLDDGGWLVTPT
                     NASLGSLDPARLSRLGPDGRLLSGDAPTKEVPLHTALYQTRRTARAVVHLHSTHSVAL
                     SMLPEIDPRAALPPMTAYYLMKCGATALVPYYRPGDPAVADAIKGLAGKYSSVLLANH
                     GPVVAGETLEAAVFAIEELEETAKLYLLLRGLNPRYLTPEQVADLSKTFGLVLPEHGH
                     D"
     gene            complement(5560..5886)
                     /locus_tag="VN968_03285"
     CDS             complement(5560..5886)
                     /locus_tag="VN968_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28304.1"
                     /translation="MNDFTTILILIVVWGAGLLFTSVRKVSYLRSIRDHLAPGAAYSD
                     TMKLGWSRRGFSFSKIDPERLTETGRIYLTRAIVNDRIMVAWLFGGFLVFACLFPHGK
                     GSGPLN"
     gene            complement(5920..6624)
                     /locus_tag="VN968_03290"
     CDS             complement(5920..6624)
                     /locus_tag="VN968_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28305.1"
                     /translation="MLSVHEVTTAYQGLVAISAVSIEVAKGEIVCVAGANGAGKSTLL
                     KSIAGAERPRSGTVTFDGARIDGLAQHLVTARGIAYVPENRRLFPRLSVRDNLRLGSY
                     LYRGETDRDAPLDLVFKLFPRLSERLDQRAETLSGGEQQMLAIGRALMTRPRLLMLDE
                     PSQGIMPKLVDEIFAAVKRIRDAGMTVLIVEQRMSECLDIADRAYILQTGRVLMQGGA
                     AEIKGNPDVRKAYLGL"
     gene            complement(6608..7339)
                     /locus_tag="VN968_03295"
     CDS             complement(6608..7339)
                     /locus_tag="VN968_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28306.1"
                     /translation="MTPLLETKGVWQRFGGLIANSDISISVGRGEIVGLIGPNGAGKS
                     TLFNLIAGVLPPTQGSIWFDGEDVTALPAAERCQRGIARTFQVVKSFETMTVIDNVIV
                     GALVRTTVMREARRKAHEVLEFAGLAGRADVSAGELIPSEKRRLEVARALATEPKLLL
                     LDEVLTGLTPIEAQTGVELVRKVRATGVTVLMVEHVMEIVMPLVDRAIVLDLGKVLVE
                     GKPADIVRDPKVISAYLGDRHAVGA"
     gene            complement(7336..8316)
                     /locus_tag="VN968_03300"
     CDS             complement(7336..8316)
                     /locus_tag="VN968_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL28307.1"
                     /translation="MDKTFAQRRRRDLVVAACLAVIAALVPLFVKDVYVQNIMVLTLM
                     YAALSQSWNILGGYCGQISLGHALYFGLGAYTTALLFTKFGVLPWFGMLGGGLISAAI
                     AMALGYPCFRLRGHYFVIATIVIAEIALLLFQNWNWAGAALGIDIPVRGDSWLKFQFT
                     RSKLPYFYFALALACVAWLVTWWLEDSKWGYWWRAVKDNPDAAESLGVVVFNSKMGAA
                     AVSAFLTAVGGSFYAQFVSYIDPESVMGFQFSLLMALPAVLGGIGTLWGPVLGAVILI
                     PLTELTRSFIGGSGRGVDLIVYGTLIVLISLARPEGLVGLFSRRRKGVAR"
     gene            complement(8316..9194)
                     /locus_tag="VN968_03305"
     CDS             complement(8316..9194)
                     /locus_tag="VN968_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL28308.1"
                     /translation="MTAETIIQSLASGLLMGLLYGLIAVGLALIFGLMDVVNFAHGEF
                     LMIAMYATFFLFAFFAVDPLLAAPLVAAALFVFGAVVYLLIVRFAVRAKANAGMVQIF
                     STFGLAIVMRGLSQYFFTPDYRSITHSWLGGKTVSIAGIFLPVPQLAGALASIAAFVA
                     LYFFINRTDFGRALEATREDAGAVALVGIDKNRVFALGWGLGAALVGLAGAIMAMFFY
                     IYPDVGASFALIAYVTVALGGFGSVFGAFAGGIVVGLVEATTALILPPSLKSVGIYAV
                     YLLVVFIRPRGLFGSL"
BASE COUNT         1824 a         3043 c         2996 g         1597 t
ORIGIN      
        1 gtcgccgcct ggatgccggt gatctgctgg ccgatctcgc cggtggcttt cgcggtctgc
       61 tcggccagcg ccttcacctc ggaggccacg accgcaaagc cgcgaccggc atcgccggcc
      121 cgtgccgctt cgatggtggc gttcagggcc agtagattgg tctgtccggc gatggtattg
      181 atgagttcga cgacgtcacc gatgcgggct gccgccttcg acaattcgct gacgcggtca
      241 ttggtcttgc gcgcctgatc gacggcgtcg gtcgccatcc gcgccgattc ctggacctga
      301 cggccgatct cgttgatcga cgaggccatc tcctcggttg cggaggccac cgaccgcacg
      361 ttggtggagg cctcctcgga cgccgccgca accacggtgg tgagtttttg cgcgcgctcc
      421 gcggtcgagg tgagattgcc ggcggaggcc tcaagttggg tcgaggccga agacaaggag
      481 tcgaccagtt cgccgatcag cgtttcgaat tcccgcgtaa tgttgtcgac gcgctggccg
      541 cgccggattt tggcgtcggc gtcaatcgcc gcagcctcgt cggaggcctt tttggcgacc
      601 agcgcgtcct tgaacacctg caacgtgttc gccatggagc cgatctcggt tgtctcgccc
      661 tgatgcggca cctgcgcggt caggtcgccg ttgcccagcg cctgcatcgg cgcgacaata
      721 gacttgattc cagcggagac gtcgcgaacc agcaggatcg cagcgccaat tcccaggata
      781 attgccacca gcagcgacgc caggaccatc ttgatggcat cgtcgaagct ttccgcggcc
      841 tggctgcccg ccgcatccgc gcccttgttg ttcagcaaga cgtccttctg cagtgtctcg
      901 tcggcatgga cgccggcaag cgacgccttg gcatggagca gcttcgcctc cttgtcctcg
      961 ttcttgcggg acagcgccag gacctccttt acgccggcga gatattcggc ccaggaggtg
     1021 ctgaactcgt cgtacagcgc gcgctcctcg gccgtggtga tcagcggttc ataagctctt
     1081 cgggccttgt cggtattgtt gatccacttg gcgagcagat cttcctgaag ctgcttgccg
     1141 gcctcctcgg ccgccagcag atgggagcgc acgatcgccc ggtaggtgat ggtcgcggcg
     1201 cgcaattcgc cgagaacgcg aatgctcggc agccaattag tcgtgatgtc gacagtggag
     1261 gaattgatcg catacatctg ccggacgttc agcgctccca ttaacgacat ggcgacgagc
     1321 agaaacgcga cgacggccgt aatcttggtg cgaatggagt atttggcgag catgaacgga
     1381 atctctcggc ttttgagcgt gttggcgctt cggtcgtgaa ccacgggatt cggcagagcg
     1441 gaggccatgc ctgtcggaat cgagccggag ggtatcgtgg ttccgttaat aattttccgt
     1501 aggactacgg tgaccgacgg gcgccgacag atgaagggcc gggtcgagct cagcgcatca
     1561 gcttcagggc atcggtgaaa aattccgggc cgccgaaatt gccggatttc agcgccagca
     1621 gcatcgcgcc cgcgttcgca ccgacggcac gcagcaccgg tacgccggcg gcgatttcca
     1681 cgcccaccag aaagcctgga atgttcaggc gatccaccac cgccccggag gtttcaccgc
     1741 cggcgaccac cagccgccgg acgccggact gcaccaggcc ttgcgcgata tcagccatcg
     1801 cctgctcgat cgcgtggccc gcggcgtcgc ggccgtggcg cgcctgcagc gccgcgacct
     1861 ggtccggcgt cgcactgctg gcgatcagga ccggaccttt accgagatgc tcattggccc
     1921 agccaagtgc gcgttgcgcc tcggctttgc cggcgactgc ctgttcggaa tcaagatgaa
     1981 acaccggcat gacccgctcg gcgttggcga tctgctgcag cgtagcctgc gaacaactgc
     2041 cggccaggca cgccgccggt ccgccgaccg cggtgtcggc catcgcgtca ggcgcatttg
     2101 atgtgaccgg gcccgaggcc gccagcgcgc gggctagacc aagcccgata ccggatgcgc
     2161 ccaccgagag ccgatgatcg agcgccacga cgccgatggt ttcgaggtcg cgggcaaata
     2221 ccgcgtccgc aaccgccgca ccaaaacccc tggtggaaag atcaaccaac tgcgcgcgga
     2281 ccgcatcggg cccgcgcgcg atgaccgcga ggtcgacgag gccgactttg gtcttgctct
     2341 gccgcgccag cacccgcacc aggttggaat catgcatcgg attgagcgga tgatccttca
     2401 gggggctctc gttgagcggc aacgcgccga cgaacagatt gccctgatag acggtgcggc
     2461 cagtttcggg aaacgcgggc gtcaccagca cgatggggtc gccggaatcg gcgcgcagcg
     2521 catccatcac cgggccgata ttgccggcgt cggtggaatc gaaggtcgaa cagatcttga
     2581 acagcacatg cgccgccccc cgcttgcgca gccatttttc cgcggcgcgc gaaccggtca
     2641 cggcaaggcc agcgtcgatc gaacggcttt tcagcgacac caccacggca tcgacttcag
     2701 gcagcgcgag atcggcagag ggaacgccga tggtctgcac cgtgcgcagg ccgcagcggg
     2761 tcagcgtatt agcgagatcg gacgcgccgg tgtagtcgtc ggcgatgcag cccagcttaa
     2821 gcgtcacggt tttactccgg cataggactt gaaccagccg aggccgtcgg tggtcatgcc
     2881 ccgcgggtta tattcgcagc cgacaaatcc gccatagtcg agccggtcca gttcctcgaa
     2941 caggaacgga tagttcaatt cttcgtcgtc gggctcgtga cgcgagggaa tgctggcgat
     3001 ctggatgtga ccgatgatcg gcatcatcgc gcgcagccgc atggtcacgt caccatggat
     3061 gatctggcaa tgatagatgt cgaactgcag tttcaggttg gggattttca gctccgcgat
     3121 caggtcgcgg gcgaacgaaa aatcgttgag gaagtagccg ggcacgttgc gcgaattgat
     3181 cggctcgatc acgacgtcta atccgtgggg agcgaaaaat tccgccgccc atgccaccga
     3241 tttagtgaac gcctggaccg cctgccgatt gcctctgtcg gcaaggcctg ccatcagatg
     3301 cagccgcttg acgccggtcg cctgcgcgta aggcagcgcg gtgcgcaggc tctgctgcag
     3361 atcgtcgaag cgctcgggca acgcggcaaa gcccttttcg ccggcgtccc aattgccggg
     3421 cggcaggttg aacagcgcct gggtcaggcc gttgcgattg agccgctcgc cgacggcctc
     3481 cgccggatgg tcatagggaa acaggaattc cacggccgtg aagcccgctc ttgccgccgc
     3541 ctcgaaacgg tcgaggaacg ggacttcggt gaacatcatg ctgagattgg cggcaaaacg
     3601 cggcatcgac ggtcctcttg cgggttcgcg gttatttcgg ctcgcttcta ttcggctcgc
     3661 tcttattcgg gttcgctctt atttgggctc gcctggcaga tgcgtgccgg tgacgcgggc
     3721 atacgtccgc gccaccgagg catcgtcgtc gcggcccatg ccggaggccg ccgtcatcag
     3781 aaacatctgc agcgcggccg ctgccaccgg caccggaaat ctcgccgtgc gcgccatgtc
     3841 ctggatgatg ccgaggtcct tgacaaagat ctccacagca cttcgcgggg agtaatcgcc
     3901 gtcgagcaca tgcgggatgc ggttctcgaa catccaggaa ttgccggcgg atgcggtgat
     3961 cacttcatag accttgcgga tatcgaggcc ctgtttggcg gcgaatgtta tcgcttccga
     4021 tgcggcggca atatgaacgc cggccagcag ctggttgatc atcttgaagg cggcgcccaa
     4081 acctgcggca tcgccgagtt cgtagagctt ggccgccatc gcatcgagcg cgggacgcgc
     4141 cttggcaaag gcggccgcac ttcccgatgc gagaatggtc agttcgccct gcgccgcgcg
     4201 ctgggcgccg ccggagatcg gcgcgtcgag ataatgccgg ccgctggctt caagctgctt
     4261 tgccaaccgc cgcgccacgt ccgggtccat cgtcgcggag gagacgaaca cggcatccct
     4321 ggccagggtt tcggcgacgc cgtccttgcc gaacagaatg gtttcggtct gcgcggcgtt
     4381 gacgaccacg ctgacgacga tgtcggccgc cttcgcggcc tccgccggtg cgtttgcacc
     4441 ccctccgccg tcagcgacga accgcgccac ggcatcggcc gagacgtcgc agccggtcac
     4501 gtcaaaaccc gcgcgccgca gcgaggtcgc catgccaaac cccatcgagc ccagcccgat
     4561 cacggcgacg cgcggttttg cggaaatgga ttcaggcata aggcatcacc ccgggggttt
     4621 cctcgccgcc gaattcaaca acggcttggc ctctgggtat cacggcttgg ccgcgctgcc
     4681 aaagcatgag acaaggcggc aaagaggatg ctgccatgac cgaaagcaat atccgcgagg
     4741 aaatctgccg cctcggccgc tcgctgttcg agcgtgggct gacgccgggt tcctcaggca
     4801 atatcagcgt cggactggat gacggcggct ggctggtaac gccgaccaat gcgtcgctcg
     4861 gctcactgga cccggcgcgg ctgtcgcgcc tcggccctga tggccgcctc ctctccggcg
     4921 atgccccgac caaggaagtg ccgctgcata ccgcactcta ccagacccgc cgcaccgcgc
     4981 gcgcggtggt gcacctgcat tcgacccatt cggtggcgct gtcgatgctg ccggagatcg
     5041 accctcgcgc cgcgctgccg ccgatgacgg cctattacct gatgaagtgc ggggccaccg
     5101 cgctggtgcc gtattatcgg cccggcgatc cggcggtggc cgacgcgatc aagggtcttg
     5161 cgggaaaata ttcgtccgtg ctgctcgcca atcacggccc ggtcgtggcg ggcgaaacgc
     5221 tggaagccgc ggtcttcgcc atcgaggaac tggaggagac ggcaaaactc tatctgctgc
     5281 tgcgcgggct gaacccgcgg tatctgacgc ccgagcaggt ggcggatttg tcaaagacgt
     5341 ttgggttggt gctgccggaa catgggcacg attaacccca catcgtcata gccgggcata
     5401 gccgtccgaa ggacggcgtc gcttccgctc gcctatgtcc cggccatcca cgtcttctgt
     5461 ctcaccgacg aagaaagacg tggatgccag gacgagcccg ggcatgacgg agagattcgg
     5521 gacacgccct ccacgtcatt gcgagcgcaa aagaagcaat caattcagcg gacccgatcc
     5581 ttttccgtgt ggaaacaggc acgcaaacac caggaagcca ccaaagagcc aagcaaccat
     5641 aatacgatcg ttgacgatcg cgcgcgtcag ataaatacgg ccggtctctg tgaggcgctc
     5701 cggatcgatt ttgctgaagc tgaagcctcg gcgggaccaa cccagcttca tagtatccga
     5761 ataggccgcc cccggcgcca aatgatcacg gatcgagcgg agataactca ccttccgcac
     5821 cgaagtgaaa agtagtccgg ccccccagac cacaatcaaa atcaggatcg ttgtgaagtc
     5881 attcatgttg cggcctggat cgttgacgct cccgctcagt cacaacccca gatacgcctt
     5941 ccgtacgtcg ggattgccct tgatctccgc ggccccgccc tgcatcagca cgcggccggt
     6001 ttgcaggatg taggcgcggt cggcgatgtc aaggcattcc gacatgcgct gctcgacgat
     6061 cagcacggtc atgccggcgt cacggatgcg ttttacggcg gcaaagatct cgtcgaccag
     6121 ctttggcatg atgccctgcg acggctcgtc cagcatcaac agccgcggcc gcgtcatcag
     6181 cgcgcggccg atcgccagca tctgctgctc gccgccggaa agcgtttcgg cgcgctggtc
     6241 caggcgttcg gacagccgcg gaaacagctt gaacaccaga tcgagcggcg cgtcgcgatc
     6301 ggtctcgccg cgatagaggt aactgccgag ccgcagattg tcgcgcaccg acagccgcgg
     6361 aaacagccgg cggttttccg gcacataggc gatgccgcgc gcggtgacca gatgctgcgc
     6421 caggccgtcg atccgcgcgc catcgaaggt gacggtgccg gagcgcggcc gttcggcgcc
     6481 ggcgatggat ttcaacaacg tcgatttgcc ggcgccattg gcgccggcga cgcagacgat
     6541 ctcgcctttt gccacttcga tgctgaccgc cgagatcgcg accagtccct gataggcggt
     6601 ggtgacttca tgcaccgaca gcatgacgat ccccaagata ggccgagatg actttcggat
     6661 cgcgaacgat gtcggcgggt tttccttcga ccagcacctt gccgaggtcg agcacgatgg
     6721 cgcggtcgac cagcggcatc acgatttcca tgacgtgctc gaccatcagc acggtgacgc
     6781 cggtggcgcg cactttgcgc accagttcga ccccggtctg cgcctcgatc ggcgtgagac
     6841 ccgtgagcac ttcatcgagc agcagcagct ttggttcggt ggcgagcgcg cgggcgacct
     6901 cgaggcgacg tttctccgac ggaatcaact cgccggccga tacgtcggcg cgcccggcaa
     6961 ggccggcaaa ttccagcacc tcatgggcct tgcggcgcgc ctcccgcatc acggtggtgc
     7021 ggaccaatgc gccgacaatg acgttgtcga tcaccgtcat ggtctcaaaa cttttcacga
     7081 cctggaaggt gcgtgcgatg ccgcgctggc agcgctccgc cgccggcagc gcggtgacgt
     7141 cttcgccgtc aaaccatatc gacccctgcg tcggcggcag cacgcccgcg atcagattga
     7201 acaaggtcga cttgccggcg ccattggggc cgatcagtcc gacgatctcg ccgcggccga
     7261 cggaaatcga gatatcgctg ttggcgatca ggccgccgaa gcgttgccag acgcctttgg
     7321 tttccagcaa gggcgtcatc gggccactcc cttgcgccgc cgcgaaaaca atccgaccag
     7381 cccctcgggc cgcgccagcg agatcagcac gatcagcgtg ccgtagacga tcagatcgac
     7441 gccgcggccg gagccgccga tgaaggagcg tgtcagctcc gtgagcggga tcaggatgac
     7501 ggcgccaagc actggtcccc ataacgtgcc gatgccgccg agcaccgccg gcagcgccat
     7561 cagcagcgag aactggaagc ccatcacgct ctcgggatcg atgtaggaga cgaactgcgc
     7621 gtaaaaactg ccgccgaccg cggtgaggaa ggccgagacc gcagcggccc ccatcttgga
     7681 gttgaacacg accaccccaa gactttcggc ggcgtccgga ttgtccttca ccgcgcgcca
     7741 ccaatagccc cacttggagt cctcgagcca ccaggtcacg agccaggcga cgcaggccag
     7801 cgccagcgcg aaatagaagt atggcagctt gctgcgggtg aactggaact tgagccagct
     7861 gtcgccacgc acgggaatat cgatgccgag cgcggcaccg gcccaattcc agttctggaa
     7921 caacagcaat gcgatctcgg caatgacaat ggtggcgatg acgaaataat gcccgcgcag
     7981 gcggaaacag ggatagccga gcgccatcgc gatggctgcc gagatcaggc cgccgccgag
     8041 catgccgaac cacggcagca cgccgaattt ggtgaacaga agggcggtgg tgtaggcgcc
     8101 aagcccgaaa tacagcgcgt ggccgagcga gatctgcccg caatagccgc cgaggatatt
     8161 ccagctctgc gacaacgccg catacatcag cgtcagcacc atgatgttct ggacgtagac
     8221 gtctttcacg aacagcggca ccagcgctgc gatgaccgcc agacacgctg ccacgaccag
     8281 atcgcggcgg cggcgctggg caaaggtctt gtccatcaga gcgacccgaa caggccgcgc
     8341 ggccggatga agaccaccag cagatagacc gcgtagatgc cgaccgattt taaggagggc
     8401 ggcaggatca gtgccgtggt ggcttcgacg aggccaacga caatgccgcc ggcaaaggcg
     8461 ccgaacacgc tgccaaaccc gcccagcgcc accgtgacat aggcgatcag cgcgaacgac
     8521 gcgccgacgt cgggatagat atagaagaac atcgccatga tagcgccggc gagccccacc
     8581 agcgccgcac ccaggcccca gcccagcgca aacaccctgt tcttgtcgat gccgaccagg
     8641 gcgaccgcgc ccgcgtcttc acgggtcgct tcaagggcgc ggccgaagtc ggtgcgattg
     8701 atgaagaaat acagcgcgac gaaggccgcg atcgatgcca gcgcgccggc gagttgcggc
     8761 accggcagga agatgccggc gatcgaaacg gttttgccgc cgagccagga atgggtgatg
     8821 ctgcgatagt ccggggtgaa gaaatactgc gagaggccac gcatgacgat ggcgagcccg
     8881 aacgtcgaga aaatctgcac catgccggca ttggccttgg cccggaccgc gaagcgcacg
     8941 atcaggagat agaccacggc accaaataca aacagcgccg ccgcgaccaa gggcgctgcc
     9001 agcagcggat cgaccgcaaa aaaagcgaac agaaagaacg tcgcgtacat cgcgatcatc
     9061 aggaactcgc catgggcgaa gttcaccacg tccatcaggc caaagatcag cgccagcccc
     9121 acggcgatca ggccgtagag cagccccatc aagaggcctg aggccagact ctggatgatg
     9181 gtctcggctg tcaaagatac gccctcaccg cacgtcgtcc ctgcgaacgc agggacccat
     9241 aatcgcagga atcgcatttg ggagaagaac gtgaggccac gatgaaaacg atactcctcg
     9301 ccgtgggtcc ctgcgttcgc agggacgacg ctatggttgt ggcaagcctt aaaaaaaaag
     9361 cgatggccta cttcatcggc caggtggctt cggcgacggc ggcttgcggc ggggagatgg
     9421 tgacgaactt gccgccgaca tattgcagca gcaccggatc
//
LOCUS       DATNOI010000096         2466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2100803,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000096 DATNOI010000000
VERSION     DATNOI010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2100803"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            422..1225
                     /locus_tag="VN968_03310"
     CDS             422..1225
                     /locus_tag="VN968_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL28309.1"
                     /translation="MFAQAPQTSAPQGAPAGAKTGYYEKGNVRIRYTEIGTGFPLLAT
                     PGGGLNSRIAVWANAVINIPEEFKNDFRVITMDQRNATGGESTGPVPVDDPWGAFADD
                     QLGLMDHLGIRQFFFFGNCIGGPFAMKLMERAPQRVVAALLSQPVGHRPEQPDYMFNA
                     GRDVWAKEFRERRRDAPMETIEKYLHSLYRVQPDFVYSVSRDFAKSCQTPMLVLPDDV
                     TAHPLQVSIDIASLAPNAEITVFPWKDPPELKARTINRARTFLKRHLPA"
     gene            1316..1474
                     /locus_tag="VN968_03315"
     CDS             1316..1474
                     /locus_tag="VN968_03315"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HXL28310.1"
                     /translation="MATPSVLKGAKPADLPVLPPTRFELMINLQTANSLGLVVPASIM
                     LRADEVIE"
     gene            complement(1703..1957)
                     /locus_tag="VN968_03320"
     CDS             complement(1703..1957)
                     /locus_tag="VN968_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28311.1"
                     /translation="MTPPVGYSDKRYFQLVDWLANEHKVLSQALRREPTDEEHRAHAH
                     AFVDLVFDDQAKAGAAWAAAAATKIVRFVPPVRPRDPWIN"
     gene            2205..>2466
                     /locus_tag="VN968_03325"
     CDS             2205..>2466
                     /locus_tag="VN968_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28312.1"
                     /translation="MSPILDLYSSRQAEAKSAADVWVYDQIPDRLRVQVSNILKDAAG
                     TSDRAVAIYQYVSREVAHEHGMTSLATKPDDPAADVHACLRTS"
BASE COUNT          448 a          811 c          755 g          452 t
ORIGIN      
        1 gtattcgcca aggctgtatt cgccaaggct gtattcgcca aggctgtatt cgccaagacc
       61 tggcggcggg catgagctga aacgacgtcc gcgctcgggg cgaagcggac ataccccgcc
      121 gtatcctcac gatcatgtcc gacgcgaatg acccacagcc gacatggggg catcgctcgc
      181 caatcgtggt gctcccggct gcatcgcctg cgcggcgcgt gcccgcccgc gccgtgagag
      241 cttgtgctac agtgtcgtgg cgcccaccgg agatcccgga agctatcagt ccaatgatgg
      301 gccgccgggg ccgagcgcat gcccgcgaca acgatacggg aggaaggcga tgatcgattc
      361 aagccgacgc actgtcctga caacaggcgc tgcggcagcg gcgacggcag ccacaccgca
      421 ggtgtttgcc caggcgccgc agacaagcgc cccgcaaggc gcgccggcgg gcgccaagac
      481 cggctactac gagaagggca acgtccgcat ccgctacact gagatcggca cgggctttcc
      541 gctgttggcg acgcccggcg gcggcttgaa ctcgcgcatc gcggtctggg cgaacgcggt
      601 gatcaacatc ccggaggagt tcaagaacga cttccgcgtc atcaccatgg accagcgtaa
      661 cgccacgggc ggcgagtcca ccggcccggt gccggtcgac gatccctggg gcgcgttcgc
      721 cgacgaccag ctcggactga tggaccatct cggcatccgc cagttctttt tcttcggcaa
      781 ttgcatcggc ggcccgttcg cgatgaaact gatggagcgc gcgccgcagc gcgtcgtcgc
      841 cgcgctgctg agccagccgg tcgggcaccg gccggagcag ccggactaca tgttcaacgc
      901 cggacgcgac gtctgggcca aggagttccg cgagcgccgg cgcgacgcgc cgatggaaac
      961 gatcgagaaa tacctgcaca gcctgtaccg cgtacagccc gatttcgtgt acagcgtgtc
     1021 gcgcgacttc gcgaagtcgt gccagacgcc gatgctggtg ctgcccgacg acgtgacggc
     1081 gcatccgctc caggtttcga tcgacatcgc ctcgctggcg ccgaacgccg agatcacggt
     1141 gtttccctgg aaggatccgc cggagctgaa ggcccgcacc atcaaccggg cgcgcacctt
     1201 cctgaagcgg cacctgccgg cgtaagcccc gacgtattgt cttgtagatc gtggcccgcg
     1261 gagcgctttc ctcaggcggg ccgcgcgttt caccgctccg cgtcgcaggt gacgtatcgc
     1321 gacaccatcc gttctcaagg gggctaagcc tgccgacctg cccgtcctac cgcccacgcg
     1381 gtttgagctg atgatcaacc tgcaaaccgc aaactcgctt ggcctagtgg taccagcttc
     1441 aatcatgctg cgcgctgacg aggtgatcga atgaggggcc gcgttgctgc gattgctcac
     1501 agccggtttt ggcactaagc ggacataccg gagacgcctg caatttgtcc gctttcggtg
     1561 cgaagcggac atgcgccgta gtgtggcttc gaccgcctcg gtcgcaaatg acccaaagcg
     1621 gacatagggc cgatcaaaat cccgcagtgc ggcagtcctg catacccgac gaagtatctg
     1681 cgaatcctct gttcgtctgc gctcaattaa tccatggatc cctgggccta acgggaggca
     1741 cgaagcggac tattttcgtc gccgcggctg cagcccaagc tgccccagcc ttagcctgat
     1801 cgtcaaaaac taggtcgacg aaggcgtgag cgtgcgcccg atgctcctcg tccgtcggct
     1861 cgcgccttag cgcctgtgac aacactttgt gctcgttcgc gagccagtcc accaattgaa
     1921 aataacgctt gtccgaatag cccacgggcg gtgtcattgt cgtcttcctc cattgcgatg
     1981 cgggcggcgg cccgatccag attggctgtg cggtagctca ggcggaaggc tgcgatttgc
     2041 tgcgtcgcat atgtcgccta tgactcgtct tctgcgcctg cggcggttat cttgccaggc
     2101 gtggcctcca cgtcaatcaa cgggtttcgg gggcacgaga tgccgggtcg accttcacgt
     2161 cagtgattcg tcagtgaaat ccccgaccgc gcgagccgta cataatgtcc cccatcctag
     2221 atctttattc gtcccgccag gccgaggcta aaagtgccgc cgacgtttgg gtctacgacc
     2281 agattcctga tcgtttgcgc gttcaggttt ccaatatcct caaggatgcc gctggtacga
     2341 gcgacagggc cgttgccatc taccaatatg tcagtcgcga ggtcgcacac gagcacggca
     2401 tgacgtcttt ggcgacgaag cccgacgacc ccgccgccga tgtgcacgcc tgccttcgca
     2461 cctcga
//
LOCUS       DATNOI010000097         9303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2456995,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000097 DATNOI010000000
VERSION     DATNOI010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9303
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2456995"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VN968_03330"
     CDS             complement(<1..587)
                     /locus_tag="VN968_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXL28313.1"
                     /translation="MNDVQDNKSPGDLGSLGRSLPGQVVLVLQGGGALGSYQAGVYQA
                     LHEAGIEPDWIIGTSIGAINASLIAGNTPQNRLSRLKQFWKRMEQNPIWSFRDVFPGF
                     NEKLSYWSTVTNGIPGFFRPNPLAHAGNDYPLGADRAGYYSTEPLEQTLRELVDFDLV
                     NRCAPRLTVGAAHVRTSQMRYFDSRDCELGVKHIMA"
     gene            complement(666..1495)
                     /locus_tag="VN968_03335"
                     /pseudo
     CDS             complement(666..1495)
                     /locus_tag="VN968_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138472.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
     gene            1677..2567
                     /locus_tag="VN968_03340"
     CDS             1677..2567
                     /locus_tag="VN968_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL28314.1"
                     /translation="MEMHQVRYFLAVARVFNFTRAADECNVTQPSLTRAIKQLEAELG
                     GDLFRRERPAAQLTELGLRMHPLLKQCYDAATGARQLASSFKSGEVGALRIALTHSVD
                     LSLLIPYLDQIKRLFNRLEFRFLRGSAREVGEFLKKGEAELGIAAEIGEEWDRLDTWP
                     LFTEGFQLVVNKRHPLADREDITFEDLQTQQLLLRSFCEHAERLSASFRGHGIDVDHS
                     HAISSERDLMELLEADIGIAVIPRSVSTPETLKRANVDGLDMRRTVHLYGVAGRERTA
                     VASTVMRMLRGADWSHYLTA"
     gene            complement(2620..3327)
                     /locus_tag="VN968_03345"
     CDS             complement(2620..3327)
                     /locus_tag="VN968_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HXL28315.1"
                     /translation="MTQLSPANAERLKLAFQQCRDMEGTLNERLGTYAAAGREIFPAY
                     GEAVDRLVARIHQNGGGENAPRPGDPMPLFILPDVTGRLVDLKSVLENGPVAVMFYRG
                     HWCPYCRLNVGAVIKAMDRIRALGGQVVAIMPEVQQFAEKFKVDSDAPFPVLTDLDNG
                     YALSLNLAIWLGTEIQRLLSYQDMKHFHGNDGWMLPIPATFVVGRDGLVKARFVDPDF
                     RKRMEIDDLIAAFRDAN"
     gene            3653..4132
                     /locus_tag="VN968_03350"
     CDS             3653..4132
                     /locus_tag="VN968_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HXL28316.1"
                     /translation="MKNRIVNLCTSLLLIFVLVPAVSPARADSDDGIIKVKSAVPISE
                     AITRVKADIAGKGIKFFMEIDQSRLAADAGIKLRPSTLLVFGNPPLGTQFITSNPNAG
                     LDWPVRLLLTQDDNGDVWAVWTDFDWIARRHNIRDRAAQFKMATMVVDSITSTITTK"
     gene            4233..4871
                     /locus_tag="VN968_03355"
     CDS             4233..4871
                     /locus_tag="VN968_03355"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL28317.1"
                     /translation="MSIETFDVVILGGGNAGIGVTGPVRRAGMSVAMIESHDLGGTCP
                     NRGCTPKKVLVAAGHALHEIERAAVHHISVGKPKLDWAALIDREKDLIKDIPANLARS
                     MAHRNVEVIKGHAVFAGLHAIRIGQRRLEARHIVIATGSKPFRRTCRPGTCQHLRAGD
                     ALWHRRRPNQGQRLRVPDVFLRYHAHDRAFLTAAPHSAALSIQKPTGISLSK"
     gene            4942..5433
                     /locus_tag="VN968_03360"
     CDS             4942..5433
                     /locus_tag="VN968_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL28318.1"
                     /translation="MFKPTTNSRARWQSLALACVIAGSAATATTAFAGECPADKMKAD
                     AREKVDFKPVGVTDVTLGSIDLEKQPAHLKDRELRFRKLTIEPGGIVPWHSHDDRPAL
                     IFVQQGEIVEYASNCSEPIVHKAGDIRPEVFGTSHWWKNLGTQTVILYVGDVRKDPHD
                     HNM"
     gene            5572..6786
                     /locus_tag="VN968_03365"
     CDS             5572..6786
                     /locus_tag="VN968_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL28319.1"
                     /translation="MTDISAEMKPETMPMHRHSPGVVLRSLIIGLTAFLTVVDLFATQ
                     AILPSLTRHYNVTPAAMGFAVNASTMGMAVAGLVVGFLSQHIDRRLGILLSLALLAIP
                     TSLLAVAPNLTVFTFLRIAQGLCMASAFALTLAYLGEQCSAMDAGGAFAAYITGNVAS
                     NLIGRLVSAAVVDNLGLASNFYFFALLNLAGAVLVYFTIQRVQPMHSMAMTQTPFAAM
                     IGHWRNPALRAAFGIGFCILFAFIGTFTFVNFVLVRPPLSLGRMDLGFVYFVFLPSIA
                     TTLLAGKAVAVAGTRPAIWGALAVAAIGLPLMLSSHLAEVLAGMVLVGAGTFFAQASA
                     TGFVGQAASENRGVAGGTYLACYFLGGLVGSAVLGQLFDRYGWIACVAGVAASLAIAA
                     LLTARLTMSGKG"
     gene            complement(6812..6952)
                     /gene="ccoS"
                     /locus_tag="VN968_03370"
                     /pseudo
     CDS             complement(6812..6952)
                     /gene="ccoS"
                     /locus_tag="VN968_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607689.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
     gene            complement(6952..7320)
                     /locus_tag="VN968_03375"
                     /pseudo
     CDS             complement(6952..7320)
                     /locus_tag="VN968_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
     gene            7346..7678
                     /locus_tag="VN968_03380"
                     /pseudo
     CDS             7346..7678
                     /locus_tag="VN968_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138450.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(7745..8923)
                     /locus_tag="VN968_03385"
     CDS             complement(7745..8923)
                     /locus_tag="VN968_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL28320.1"
                     /translation="MRGLDWFIFFLADVQTGFGPFIAVYLTTQKWTQVEIGLVLSIGG
                     LVGLIGQMPGGAIVDAARSERLVAGLAVATIGVCALGYAAWPIFPVVVVAATLHAAAS
                     CVLGPAIAAISLGLVGPLAIGERLGRNARYASLGNGVAAAVMGTCGYLLSSRSVFLVT
                     FILAIPTLLALGRIREWEIDAAQAHGAVAREVPDANATSVLNLMCRRPLLIFAGSVFL
                     LQLANAAMLPLMAGVVTTRSSQWAPVLIAACIVVPQAIVALTSPSVGRKAQLWGRRPL
                     LLLGFAALAIRGVLFATVSDPYLLVAVQVFDGITAAVFSVMVPLIIADVAFGSGHFNL
                     AQGIVGTATGIGASLSTVLAGYVSDRFGSSIAFTGLATVAATGLLLIWLVMPETRRTT
                     "
     gene            complement(9074..>9303)
                     /locus_tag="VN968_03390"
     CDS             complement(9074..>9303)
                     /locus_tag="VN968_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRC-barrel domain containing protein"
                     /protein_id="HXL28321.1"
                     /translation="VRAAVIDFGGFLGVGSRKIVVDWNALHFGRIANKSDSITLELTK
                     DQVTAAPEYKEDTPIVVLGASGSLQPLQFGP"
BASE COUNT         1790 a         2950 c         2804 g         1759 t
ORIGIN      
        1 gccatgatgt gcttgacgcc aagttcgcaa tcgcggctgt caaaatatcg catctggctg
       61 gttcggacat gcgcggcgcc gaccgtcagc cgcggcgcgc agcgattgac caggtcgaaa
      121 tcgaccagct cgcgaagggt ctgctcgagc ggctcggtcg aataatatcc ggcccggtcg
      181 gcaccaagcg gatagtcatt gccggcatgg gccagcggat tgggcctgaa aaaccccgga
      241 attccgtttg tcaccgtcga ccagtacgac agtttttcat tgaagccggg aaatacatca
      301 cgaaaactcc agatcggatt ctgttccatc cgcttccaga attgctttag ccgcgacagg
      361 cgattttgcg gcgtgttgcc ggcgatcaga ctggcattga tggcgccgat cgatgtgccg
      421 atgatccagt ccggctctat accggcctcg tgcagcgcct gatagacgcc ggcctgataa
      481 gatcccagcg cgccaccacc ctgcagcacg agcacgacct gaccgggaag cgacctgccc
      541 agtgatccca agtcccctgg agatttgttg tcctgaacgt cgttcatgtc acgctcccgt
      601 tggatgtacg ccgcgttccc tgttgttcgc gggcggcttg aatttgttac gttggctggc
      661 ctatttcagt gcgcggtcca gccgccatcg accagcaggg ctattccagt gatcgaggaa
      721 gccgcgtcgc cggcgagaaa taccgtgagc gcgccgagtt cctcgacggt cgcaaagcgc
      781 ttgttgggtt gctgcgccag caagacatca cggatgacct gctcgcggga aattccaagc
      841 gccttggcct ggccgtcgat ctgcgcttcg accagcgggg tgtagacata gcccgggcag
      901 atcgcgttgc aggtgatgcc tcttcggcgg tctcaagggc agcgaccttg gtcgggccga
      961 cgatgccgtg cttggcggcg acataggccg ccttgaacgg cgaggcgacg agcccgtgcg
     1021 cggacgcgat gttgatgatg cgtccaaact tgttcttgcg catcgcgggc agcgccagcc
     1081 gcatgctgtg aaacgccgac gacaggttga tcgacaggat cgcgtcccat ttttcgatcg
     1141 gaaaccgatc gagcggggcg acatgttgaa tacccgcatt gttgacgagc acgtcgagcc
     1201 ggccatgttc ggcaagcgca gtcgcgatca tgtcggcgat cgcctccggc ttcgtcatat
     1261 ccgcggccga atagctcacc ttcacaccga agtcggccgc tatctggttt ttggtcttgg
     1321 cggtctcggc cgcaaggccg aacccgttca gcaccacggc cgagccggcc tcggccagcg
     1381 cgcgggcaat accgagcccg ataccgctgg tggagccggt taccagcgag actttgccga
     1441 tgagcgatcg cgaggccgat gacgaagcgc tttcggtttt caactggatg ttcatggcag
     1501 tctccttcgt ggatggcttg gcgacttcac cgcctgacca agttctgcac gatggggtca
     1561 atcagcggaa atgccctttg gcttccaaaa ccataggctg gcgctatcgc ggtttgggtg
     1621 tcattgccga gcgttctcag ctagttttgc cggcacaatc cgatcggggc acgatcatgg
     1681 aaatgcatca ggttcgctac ttcctcgctg tcgcgcgcgt gttcaacttc acgcgcgccg
     1741 cggatgagtg caacgtgacc cagccgtcgc tgacgcgggc gatcaagcag ctcgaggcgg
     1801 aactcggcgg cgatctgttc cggcgcgagc gaccggcggc gcaattgacc gaacttggcc
     1861 tgcgcatgca cccgctgctc aagcagtgct atgacgccgc gaccggagcc cgacaactgg
     1921 cgtcatcatt caagagcggc gaagtcggcg cgctcaggat cgccctcacc cattctgtcg
     1981 acctgtcgct gttgatcccg tatctcgacc agatcaagcg gctgttcaac cggcttgaat
     2041 tccgcttcct gcgcggcagc gctcgggaag tcggggaatt tctcaagaag ggcgaagccg
     2101 agctgggcat tgccgccgaa atcggcgaag aatgggaccg gctcgatacc tggccgctgt
     2161 tcaccgaagg ttttcaactc gtcgtcaaca agcgccatcc gctggcggac cgtgaagata
     2221 tcaccttcga ggatctgcaa actcagcaat tgctccttcg aagtttctgc gagcatgcgg
     2281 agcggctgag cgcatcgttt cgcggccacg gcatcgacgt agatcacagc catgcgattt
     2341 catcggaacg cgacctgatg gagctgctcg aagccgatat cggcatcgct gttattccgc
     2401 gcagcgtatc gacgccggaa accctcaaac gggcaaacgt cgatggactc gatatgcgcc
     2461 gtaccgtcca cctctatggc gtcgccgggc gcgagcggac tgccgtggcg tcaacggtga
     2521 tgaggatgtt gcgtggcgcc gactggtcgc actatctgac cgcgtgagtg ggcaacgccg
     2581 gcgacgcaac cgataaaccg gcggctctaa agatctgatc tagtttgcgt ctctgaacgc
     2641 ggcgatcagg tcgtcgattt ccatgcgctt gcggaagtcg ggatccacga accgagcctt
     2701 caccagcccg tcgcggccga ccacgaacgt cgccggaatc ggaagcatcc agccgtcatt
     2761 gccgtgaaaa tgtttcatat cctgatacga cagcagccgc tgtatttcgg ttccaagcca
     2821 gatcgcgaga ttcaatgaaa gcgcatagcc attgtcgaga tcggtgagaa ccgggaacgg
     2881 cgcgtcggaa tcgactttga acttttcagc gaattgctgc acctcgggca tgatcgcgac
     2941 aacctgcccg cccaacgccc tgatccggtc catagccttt atcaccgccc cgacgttcag
     3001 cctgcaatag gggcaccaat gcccccgata gaacatcacg gcgaccggtc cgttttccag
     3061 aacggatttc agatcgacca gccgcccggt cacatccgga aggataaaga gcggcatcgg
     3121 atcacccggc cgcggcgcgt tttctccgcc gccgttttgg tggatccgcg ccaccaggcg
     3181 atcaaccgct tcaccatagg ccggaaatat ctcccgcccg gcagcggcat aggtgcccag
     3241 gcgttcgttc agcgtcccct ccatgtcgcg gcactgctgg aaggccagct tgagacgctc
     3301 ggcattggca ggtgaaagct gggtcatgtc ttgctccggc ggattgggct ttccagattc
     3361 ggactgaaac atatggcggc accccacgac accgtaatcg gatttctcgg acattgtacc
     3421 attttcccgc acacgccgcc ctcaaggggg acttacgcat tagaggctga atggcttgat
     3481 gttggaaatg ccgttgcaca ataggtttga tagccaggga gcatcgcccc gcccctaata
     3541 gcgcgcagct atcgacttca aagcgagtgc gcatttcata ttgctgaatt gcgcgttcat
     3601 cctgtctccg acgcgatgcc atcaacattt gctcgcgaac aggagacgtc agatgaagaa
     3661 ccgcattgta aatctctgca catcactgct gttgatattc gtcctggtcc cggctgtctc
     3721 tcctgcaagg gcggacagcg acgacggtat cataaaggtc aagagcgcgg tccccatctc
     3781 ggaagcgatc acccgcgtca aggccgacat cgccggtaag ggcatcaagt tcttcatgga
     3841 aatcgaccag tcgaggcttg cggccgacgc cgggatcaag cttcgtccct ccaccctcct
     3901 ggtattcgga aatccgccgc tcggaaccca gttcattacg tcgaatccga atgccgggtt
     3961 ggattggccg gtccggctgc tgctgacgca ggacgataac ggcgatgtct gggcggtctg
     4021 gaccgatttt gactggatcg cccgacgcca caacatcagg gatcgcgccg cacagttcaa
     4081 gatggcgacc atggtcgtcg actccatcac atcgaccatc acgaccaagt aacaacaccc
     4141 gcacaccgcg cgtaatagct tcgggccgcg cccatgacgc ggcctgaccg cgcgacgtcc
     4201 cggggatcaa ctacgacata gggatatttc cgatgagtat tgagacattc gacgtggtga
     4261 tcctgggcgg cggtaacgcc ggcattggcg ttaccgggcc agtcagacgc gccggaatgt
     4321 cggtcgcgat gatcgaatcg cacgacctcg gcggaacctg tccgaaccgt ggctgcacgc
     4381 caaagaaggt gctggtcgcg gccggtcacg ccttgcacga gatcgagcgc gccgccgttc
     4441 accacatctc ggtcggcaag ccgaagctcg actgggccgc gttgatcgac cgcgagaagg
     4501 acctcatcaa ggatatccct gcgaaccttg cccgctcgat ggcgcatcgc aatgtcgagg
     4561 tgatcaaggg ccacgcggtt ttcgctgggc tccatgcgat ccgcattggc cagcgtcgcc
     4621 tcgaggccag gcacatcgtg atcgcgaccg gatcgaagcc atttcgtcgg acatgccggc
     4681 caggaacttg tcaacatctt cgggctggcg atgcgctttg gcatcgccgc cggccaaatc
     4741 agggacaacg tctacgcgta cccgacgttt tcctccgata tcacgcacat gatcgggcat
     4801 tcctgacggc cgctccacat agcgcggcgc tatcgattca aaagccaacc ggcatttcct
     4861 tgagcaagtg atttcaacga agcttcgtcc tgtagccggc cagatcatcc gaggccacgg
     4921 cataacagag gagataacga catgttcaag cccaccacga attcacgcgc gcgctggcaa
     4981 agccttgcac tcgcctgcgt catcgcggga tcggcagcaa cggcgacgac cgctttcgcc
     5041 ggcgaatgtc cggccgataa aatgaaggcc gacgctcgcg agaaagtcga cttcaagccg
     5101 gtcggcgtca ccgacgttac gcttggttcg atcgatctcg aaaagcagcc ggcgcatctc
     5161 aaggatcgtg agctgcgttt ccgcaagctg acgatcgagc ccggcggcat cgtgccctgg
     5221 cacagccatg acgatcggcc tgccctcatc tttgtgcagc agggcgagat tgtcgaatac
     5281 gccagcaact gttccgagcc cattgtgcac aaggccggcg atatcaggcc cgaggtgttc
     5341 ggcacttcgc attggtggaa gaacctcggg acccagacag tcattctgta cgtcggcgac
     5401 gtccgcaagg atccgcacga ccacaatatg taacgcggat gtaacgagcg cgcgccaagt
     5461 cgttacgagc ctcgttgtct gatgtgacgt cccggcagcc cccgtattgc cccgcacgcc
     5521 cgggcgtcac atccccttct tgaacccagc tttcgcagga agcaccgtgc catgaccgac
     5581 atatccgcgg agatgaagcc cgaaaccatg ccaatgcacc gccattcgcc gggcgtggtg
     5641 ctgcgttccc tgatcatcgg cctgacagcc tttttgacgg tcgtcgatct gtttgcgacc
     5701 caggcaattc ttccttcgct gaccaggcat tacaacgtga cgccggcggc gatgggattt
     5761 gcagtcaatg ccagcaccat ggggatggcg gtcgctggac ttgtcgtcgg attcctgagc
     5821 cagcacatcg atcggcggct cggcattttg ctgagtcttg ccctgctcgc catcccgaca
     5881 agcctgctgg cggtcgcgcc taatctgact gtgttcacgt ttcttcgtat cgcgcagggt
     5941 ttgtgcatgg catcggcgtt cgcattgacg ctggcctatc tcggcgaaca atgcagcgcg
     6001 atggatgccg gcggcgcgtt cgcggcctac atcactggca acgtcgccag caatctgatc
     6061 ggccggctgg tttccgcagc cgtcgtcgac aatctcgggt tggcctcgaa tttctatttc
     6121 ttcgcgttgt tgaatctcgc cggagcggtg ctggtgtact tcaccatcca gcgggtacag
     6181 ccgatgcatt cgatggcgat gacgcaaacg cctttcgcgg cgatgatcgg acactggcgc
     6241 aacccggcgc tgcgcgccgc cttcggcatc ggcttctgca tcctgttcgc ctttatcggg
     6301 acattcacct tcgtgaattt tgtcctggtg cgtccgcccc tgtcgctcgg acgtatggac
     6361 ctcggatttg tctattttgt cttcctgccc tctatcgcga cgacgcttct tgccggcaag
     6421 gctgtcgcag tcgccggaac gcggccggcg atctggggcg cgcttgcggt cgcggcgatc
     6481 ggcctgccgc tgatgctgtc ctcgcatctg gcggaagtcc tggccggcat ggttctggtc
     6541 ggcgccggca cgttctttgc gcaagcctcg gcaaccggtt ttgtcggaca ggccgccagt
     6601 gaaaaccgcg gcgtagcggg cggaacctac ctcgcctgct atttcctggg cggtctcgtc
     6661 ggcagcgcgg tcctgggcca attgttcgac cgctatggat ggatcgcctg cgtcgccggc
     6721 gtggctgcgt cgctggcaat cgccgcgcta ctgaccgcac gcctgacgat gtcaggcaaa
     6781 gggtaagcgc acttaatccg acggcgtctc ccggcccgtc atcctggatg gcgcgccagg
     6841 cggcgccctc gagatcgtcg tactgaccgc tcttgagcga ccagagaaat ccgagcaggc
     6901 cgaccagccc gagaccaaga gccaacggca ccagaatgac gagcacttcc atcacgcgtc
     6961 tccgtgcacg acgcggcgcg cgcgaagcgc gttcagcatc accagcagcg aggagcccga
     7021 catcgccgcc gctgcgatca ggggtgtcac gaccccgccg atcgcaattg gcaccgcgag
     7081 aaggttgtag ccgaccgcga gccagagatt ttgccgcatc agatgcagcg cttttcgcga
     7141 aaagtcgatg gcagcgacca ccggagccag cggcttgccc aggaacacca gatcggccgt
     7201 cgcctggctc agatgcgccg ccgagatcgg cgacatcgag acgtaggccg cggccagtga
     7261 tggcgcgtcg ttcaacccgt cgccgaccat catcacccta aaaccttggc tcttcaggtc
     7321 tacatcgcac cggatgcaac cgtctgccgc cggggtagat gtcaatgtcg cgcgcagcct
     7381 ttggaccatg accgcgggcg agcttcgaca cgcggaagat cacatgcagc tgctcgggcg
     7441 caagaccgcg atggagcgcg tagccacctt cctgttggaa atggatcgcc ggcttgcggt
     7501 cgcggggatg atggcgctgc cgatgtgccg ccgcgatatc ggcgattatc tcggcctcac
     7561 gctggaaacc gtctcgcgag cgctttcgca gctgcacgcg caaggggtcc tcggattttc
     7621 cggcgcgcgg cagatcgtgc tccgcaaccg tcagcggctg cgccacatgg atgcgtgaga
     7681 atgatagctg gattttgacg cgtttgcttg acgcgaaccg gtctccactt cgcttgaaaa
     7741 cgctctatgt ggtccgccgc gtttccggca tcacaagcca gatcaatagc aggcccgtcg
     7801 ccgcaacagt ggcaagcccg gtgaaagcga tactgctgcc gaacctgtcg ctgacgtagc
     7861 cggcgagcac ggtgctaagg gaagcgccga tgccggttgc cgtgccgacg atgccctggg
     7921 cgagattgaa gtggccgctt ccgaacgcga cgtcggcgat aatcaacggc accatgacgc
     7981 taaagaccgc cgcggtgatg ccatcgaaca cctgcaccgc gaccagcaaa tacggatcgc
     8041 tcacggtggc gaatagcacg ccgcggatcg ccagcgcggc aaagcccagc agcagcagcg
     8101 gccgacgccc ccatagctgc gccttgcggc caaccgatgg agacgtcagc gcgacaatgg
     8161 cttgcggcac cacgatgcat gcggcgatca gcaccggcgc ccactggctg gaccgggtgg
     8221 ttaccacgcc cgccatcaac ggcagcatcg ccgcgttggc gagctgcagc aagaacacgc
     8281 tgccggcgaa gatcaacagg ggacgccggc acatcagatt cagtacgctc gtggcgttgg
     8341 cgtcgggcac ctcgcgcgcc accgcgccat gcgcctgggc ggcgtcgatt tcccactcgc
     8401 ggattcgtcc gagcgcgagc agggtcggaa tcgccagaat gaaggtgacg agaaacaccg
     8461 agcggctcga gagcagatag ccgcacgtgc ccatcacggc cgcggcgacg ccattgccga
     8521 gtgatgcata gcgggcgttg cgtccgagcc gctcgccgat cgccagcggt ccgaccaggc
     8581 cgaggctgat cgcggcaatc gccggaccaa gcacgcaact tgcggcggcg tgcagggtcg
     8641 ccgcgaccac caccaccgga aagatcggcc aggcggcata tccgagcgcg cagacgccaa
     8701 tcgtcgccac cgcaaggccc gccaccagcc gctcggagcg ggcggcgtcg acgatggcgc
     8761 cgccgggcat ttgtccgatc agcccgacaa ggccgccgat ggaaagcaca agaccgatct
     8821 caacctgggt ccacttttgc gttgtcaggt agaccgcgat gaaggggccg aatccggtct
     8881 gcacatccgc gagaaaaaag atgaaccaat caagtccgcg caagctctgg cgcgagggag
     8941 ccggcgccgc cgcagccgtc ttcggctctg gcggcgcaac ggtttcagca ccgaccggcc
     9001 gctcacggcg gtcacgatca attcggtcgc gcgaatgtcg ggatggacgt gcgaacagga
     9061 tatctctccg cacttaagga ccaaactgca gtggctgcag gctccccgat gcgcccagca
     9121 cgacgatcgg cgtatcctcc ttgtactctg gcgccgccgt cacctgatcc ttggtcagtt
     9181 cgagggtgat gctgtcgctc ttgttggcga tgcgtccgaa atgcagcgca ttccagtcga
     9241 cgacgatttt ccggctgccg acgccgagaa aaccgccgaa gtcgatcacg gccgcgcgca
     9301 ccg
//
LOCUS       DATNOI010000098        11519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1013478,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000098 DATNOI010000000
VERSION     DATNOI010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11519
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013478"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VN968_03395"
     CDS             <1..1111
                     /locus_tag="VN968_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXL28322.1"
                     /translation="QLKRGMGVMQKNYEATAARGGIPADAPAKRMGLINGVVGLENVK
                     DADLVIEAVFETMAIKKEVFGQLDKFAKPGAVLASNTSYLNIDEIAKETKRPQDVLGM
                     HFFSPANVMKLCEIVRAARTAPDALTTAVAVARRIAKVPAVVGVCDGFVGNRMLAQRG
                     KQAEKLLFEGALPQQVDAVVTKFGMPMGPFAMGDLAGLDIGWRSRKDRGIKSEIADAL
                     CEAGRFGQKTGKGYYKYEGGSRAPLPDPEVEKLIDETLQRLGRKRRAVSDEEILERMM
                     YPMINEGARILEEGIAARPSDIDVIWIYGYGWPVYRGGPMYYADQVGLKHIADRLSYY
                     AKETNDPSLEPAPLLKRLAAEGKTFASLAERAKAA"
     gene            1293..2975
                     /gene="pimA"
                     /locus_tag="VN968_03400"
     CDS             1293..2975
                     /gene="pimA"
                     /locus_tag="VN968_03400"
                     /EC_number="6.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate--CoA ligase PimA"
                     /protein_id="HXL28323.1"
                     /translation="MTYPGEKFYPEGVHWDDPIAQGTLPDLLSNAATQYGARPAIEFR
                     DRPISYAELESLVEVAASAYLRAGYGKDTSVAVFLGNTPDHPINFFAALKAGSRIVHL
                     SPLDGEIALSHKLSDSGARVLVTSNLAALLPTALKFLDKGLLDRLIVCEDDHWGKIGT
                     PQTPLPDNPAIITHRQFIDGATRPAPWPAIDVEDIALLQYTGGTTGLPKGAMLTHGNL
                     TAAVSIYDVWGKAARAERGDPVERVICVLPLFHIYALTVVMLSCIRRGHLISLHQRFD
                     VESVMRDIEVKRATYFPGVPTMWIAIAALPDLDKRDFSSLRSAGSGGAPLPMEVARVF
                     ERKVGMKLRSGWGMTETCPAGTAHPEAGPDKPGSVGVALPGIEMDVVSLDDPTKVLPT
                     GEVGEIRIRGPNVTKGYWNKPKETAEAFVGDRFLTGDIGYVDDEGYFFLVDRKKDMII
                     SGGFNVYPQMIEQAIYEHPAVHEVIVIGISDDYRGEAAKAFVKLRTGANPFTLDELKA
                     FLAGKLGKHEIPAALDFVDELPRTTVGKLSRHELRKLQPAQAKQQQVASGGR"
     gene            3160..4350
                     /gene="pimC"
                     /locus_tag="VN968_03405"
     CDS             3160..4350
                     /gene="pimC"
                     /locus_tag="VN968_03405"
                     /EC_number="1.3.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018515 - pimeloyl-CoA dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-CoA dehydrogenase large subunit"
                     /protein_id="HXL28324.1"
                     /translation="MDLSFTREEMAFRDEVRAFFRDNVPSATRQKLQEGRHLSKQEMV
                     DWWRILNKKGWGVTHWPKEYGGTGWSSVQHYIFNEELQMHPAPAPLAFGVSMVGPVIY
                     TFGNEQQKKYYLPRIANVDDWWCQGFSEPGSGSDLASLKTKAERKGDKYIVNGQKTWT
                     TLAQHADMIFNLCRTDPAAKKQSGISFILIDMKSKGITVRPIQTIDGGHEVNEVFFDD
                     VEVPVENLVGEENKGWDYAKFLLGNERTGIARVGVSKERIRRIKELASKVESGGKPVI
                     EDQKFREKLAEVEIELKALELTQLRVVADEGKHCKGKPNPASSVLKVKGSEIQQATTE
                     LLMEVIGPFAAPYDVHGDDGSNEAMDWTAQIAPSYFNNRKVSIYGGSNEIQRNIITKA
                     VLGL"
     gene            4670..5812
                     /gene="pimD"
                     /locus_tag="VN968_03410"
     CDS             4670..5812
                     /gene="pimD"
                     /locus_tag="VN968_03410"
                     /EC_number="1.3.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018515 - pimeloyl-CoA dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-CoA dehydrogenase small subunit"
                     /protein_id="HXL28325.1"
                     /translation="MDFDLSEEQRLLKESVDGLLGSSYEFEQRKKYMAEKGGWSRSAW
                     NKLAEQGLLGLPFSEDDGGFGAGAVETMIVMEALGKALVLEPYLATVVLGGGLLRRGG
                     SAEQKAAHIPSIIDGSKTLAFAQLEKNSRYDLNDVSTSAKKKGSGYVIDGEKFVVLNG
                     ESADTLIVTARTKGGQRDTGGIGVFLVPGNAGGVAKKGYPTQDGLHAADISFTGVEVG
                     ADAAIGDPDNALPLIERVVDDARIAICAEAVGIMDESLKTTVEYLKTRTQFGVPIGTF
                     QTLQHRAADMFVALEQARSMSMFATMASDFDDARERATAVAAAKVQIGKSGKFIGQQS
                     IQLHGGIGMTQEARIGHYFKRLTMIENTFGDTDYHLRRVTDSGGLV"
     gene            6066..6839
                     /locus_tag="VN968_03415"
     CDS             6066..6839
                     /locus_tag="VN968_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL28326.1"
                     /translation="MKTTPFDLTGKVAVITGSSRGIGRASAELLAKLGARVVISSRKA
                     DACEEVAAGIRKAGGDAHVIPCNISRREEVEALTSGATRHYGRIDILVCNAAVNPYYG
                     PLLDITDEAFDKIMGSNVKSNIWLCALAIPPMAERGNGSVVIISSIGGLRGSTVIGAY
                     GISKAADFALCRSLAGEWGPKGVRVNCVAPGLVKTDFARALWEDEERLKRRCATTPLR
                     RIGEPDEIAGAVAYLSSDAASFMTGQTIVVDGGVTTASV"
     gene            7080..8582
                     /gene="glpK"
                     /locus_tag="VN968_03420"
     CDS             7080..8582
                     /gene="glpK"
                     /locus_tag="VN968_03420"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HXL28327.1"
                     /translation="MSHVLAIDQGTTSSRAIVFRSDISIAASAQQEFPQHFPASGWVE
                     HEPEDIWISTIATCREALKKAGVGAKDIAAIGITNQRETAVVWDRATGQAVHRAIVWQ
                     DRRTADICARLKAEGHESAITEKTGLIIDPYFSGTKVAWILDSVPGARERAGRGELMF
                     GTVDCYLLWRLTGGKVHATDATNASRTLLFNIHTGQWDDDLLKILRVPGSMLPEVKDS
                     STKFGDSVPELFGGAIPISGIAGDQQAATIGQACFAPGMIKSTYGTGCFALLNTGTTP
                     VASKNRLLTTIAYQLKGQRTYALEGSIFVAGSSVQWLRDGLGIIKQASETGALADQSD
                     STQSVYLVPAFVGLGAPYWNPRVRGALFGLTRNTGPAELAHAALESVCYQTFDLRAAM
                     RADWPEAKAANVLRVDGGMTASDWTMQRLADLLDAPVDRPMIQETTALGAAYLAGLAA
                     GVYPEPEKFADNWRLEHRFKPNMSAATRERKLKGWARAVKGLLASDEGEG"
     gene            complement(8715..9134)
                     /locus_tag="VN968_03425"
     CDS             complement(8715..9134)
                     /locus_tag="VN968_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28328.1"
                     /translation="MFNRRTIVKLVVCTSVLVTAGFALAKNPHHNNGHNLLGPKLHKN
                     GKHEVGKIGGNAVVAEVNNDKVVGMSAGSLPARKVKSNKKLASGDTGIVKIAANGPIQ
                     LAQADFYYGYCFDDGIDEYCYWYPAYDVIVTDPWAPY"
     gene            9321..10493
                     /locus_tag="VN968_03430"
     CDS             9321..10493
                     /locus_tag="VN968_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28329.1"
                     /translation="MRRQIIAQQLEAALRNSLQESGLRLVSLDGERPSVDTRREPWIE
                     IKYGPASPDDWFGIVPATAQFERSAGKTREALNLIVKVNPRLGLARNLIPWIVENKSI
                     RLDRPYWEYRTSAETDRTADREFHIYELAATGSSALSWVLPRYYGAASDPATGERALF
                     MERLTDVSRLDAAGVQADWPIEAIGAAFAAAAAWQAEFWGAGNASLAWAGPRPTTDDM
                     LADESLWRGLLDDAHARFPAVITEEVRSRRHRLIDTMDVWHRAKDLLPGTLAHDDFNN
                     RNVGFRPGVVALDWELAQCNTAHRDLVEMLTFVAPSAATRADIDRLMERHRTMLAETG
                     VKAGLDRDTWMEAFRCELKVEAINRISMQCIFEAAFPLAYFERINTNIERLLDLYG"
     gene            10667..11299
                     /locus_tag="VN968_03435"
     CDS             10667..11299
                     /locus_tag="VN968_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXL28330.1"
                     /translation="MILIGQYDSPFVRRVAIALRLYELSFEHRPWSTFGDGEKIAPYN
                     PLRRVPTLVLDGGEALIESTAILDYLDEVAGPDKAMIAARGPERRRALKICALASGLG
                     DKGVSLLYERVLRKDQSKIWVERCEAQIFSVLDVLEQERTAVKTPYWFGERIGHADVA
                     VACALRFTSEAHPQLFDAARHPALAAHAAQCEALPPFKEIVQPLAPPGAD"
     gene            complement(11309..>11519)
                     /locus_tag="VN968_03440"
     CDS             complement(11309..>11519)
                     /locus_tag="VN968_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28331.1"
                     /translation="AFQRRASAHFHRRSRKRKIATSAITRVYAEKAAQRNGSVRLAQL
                     WGPLKWMFVAFGAWRAPNRPSGALS"
BASE COUNT         2216 a         3632 c         3655 g         2016 t
ORIGIN      
        1 gcagctcaag cgcggcatgg gcgtgatgca gaagaattac gaggcgaccg cggcgcgcgg
       61 cggcattccg gccgacgcgc cggccaagcg catgggcctg atcaacggcg tggtcggcct
      121 cgagaacgtc aaggacgcgg acctcgtcat cgaagccgtg ttcgagacca tggcgatcaa
      181 gaaagaggtg ttcggccagc tcgacaagtt tgccaaaccg ggcgcggtcc tcgccagcaa
      241 cacctcctac ctcaacatcg atgagatcgc caaggagacc aaacgcccgc aggacgtgct
      301 cggcatgcac ttcttctcac cggcgaacgt gatgaaactt tgcgagatcg tgcgcgcggc
      361 caggaccgcg ccggatgcgc tcactaccgc ggtcgcggtg gcgcgcagaa ttgcaaaagt
      421 gccggcggta gtcggggttt gcgacggctt tgtcggcaat cgcatgctgg cgcagcgcgg
      481 caagcaggcg gaaaagctgc tgttcgaagg cgcactgccg cagcaggtcg acgcggtcgt
      541 gaccaagttc ggcatgccga tggggccgtt cgcgatgggc gatctcgccg gcctcgatat
      601 cggctggcgc tcgcgcaagg atcgcggcat caagtcggag atcgccgatg cgctgtgcga
      661 ggctggccgc ttcggccaga agaccggcaa gggctactac aagtatgaag gcggctcgcg
      721 cgcgccgctg cccgatccgg aagtcgaaaa actgatcgac gagacgttgc agcggctcgg
      781 gcgcaagcgc cgcgcggtca gcgacgagga gattctcgag cgcatgatgt atccgatgat
      841 caacgagggc gcgcggattc tcgaggaggg catcgcggcg cggccgagcg acatcgatgt
      901 gatctggatc tacggctatg gctggccggt ctaccgcggc ggcccgatgt attatgccga
      961 ccaggtcggc ctcaagcaca tcgccgaccg gctgtcgtac tatgccaagg agaccaacga
     1021 cccctcgctc gaaccggcgc cattgctcaa gcgcctcgcc gccgaaggca agacctttgc
     1081 gtcgctggcc gagcgagcga aagcggcttg atggcgatga gctgcatgcg ttcggtgtct
     1141 ccgttccctc cccccttgcg ggggagggtc agggaggggg gtggcggcat cggcaccgcc
     1201 cgtgtggacc cccaccccca acccctcccc acaaggggga ggggagcctg tgcagttctg
     1261 ctcgcctcac ggcagaaacg agcaacgcgc cgatgaccta tcccggcgaa aaattctatc
     1321 cggaaggcgt ccactgggac gacccgatcg cgcagggaac gctgccggac ctgttgtcga
     1381 acgccgccac gcaatacggc gcgcggccgg cgatcgaatt ccgcgatcgc cccatcagct
     1441 atgccgagct ggaatccctc gtcgaagtcg cggcgtccgc ctatttgcgc gcgggctatg
     1501 gcaaggacac ctccgttgcg gtgttcctgg gcaacacgcc cgatcatccc atcaatttct
     1561 tcgccgcgtt gaaggcaggc agccgcatcg tgcacctctc gccgctcgac ggcgagatcg
     1621 cgctgtcgca caaactttcc gattccggcg cgcgggtgct cgtcaccagc aacctcgcgg
     1681 cgctgttgcc gacagccctg aaattcctgg acaagggcct gctcgatcgc ctgatcgtct
     1741 gcgaggacga tcattggggc aagatcggca ccccgcaaac gccgctgccg gacaatcccg
     1801 ccatcatcac ccacaggcag ttcatcgatg gcgcgaccag accggcgcca tggccggcca
     1861 tcgacgtcga ggatattgcg ctgctgcaat ataccggcgg caccaccggc ctgccgaaag
     1921 gcgcgatgct gacccacggc aatctcaccg ccgcagtctc gatttatgac gtctggggca
     1981 aggccgctcg cgccgagcgg ggcgacccgg tcgagcgcgt gatctgcgtg ctgccgttgt
     2041 tccacatcta tgcgctgacg gtcgtgatgc tgtcctgcat ccgccgtggc cacctgatct
     2101 cgctgcacca gcgcttcgat gtcgaaagtg tgatgcgcga catcgaggtc aagcgcgcga
     2161 cttatttccc gggcgtgccg accatgtgga tcgcgatcgc ggccttgccc gatctcgaca
     2221 agcgcgattt ctcgtcgcta cgaagcgccg gctcgggcgg cgcgccgctg ccgatggaag
     2281 tcgcgagggt ctttgagcgc aaggtcggca tgaagctccg tagcggctgg ggcatgaccg
     2341 aaacctgccc ggccggtacc gcgcatcccg aagcgggacc ggacaagccc ggctcggtcg
     2401 gagtggcctt gcccggcatc gagatggatg tggtgtcgct tgacgatccc acaaaggttt
     2461 tgccgaccgg cgaggtcggc gagatccgca ttcgcggccc gaacgtcacc aagggttact
     2521 ggaacaagcc gaaggagacc gcggaagcct ttgtcggcga ccgcttcctc accggcgaca
     2581 tcggctatgt cgacgacgaa ggctatttct ttctggtcga tcgcaagaag gacatgatca
     2641 tttccggcgg cttcaacgtc tacccgcaga tgatcgagca ggcgatctac gagcatcctg
     2701 cggtgcacga agtaatcgtg atcgggatat cagacgatta ccgcggcgag gcggcgaagg
     2761 cctttgtcaa attgcgcact ggggccaatc cgttcacgct ggacgagttg aaggcatttt
     2821 tggccggcaa gctcggcaaa cacgaaattc ccgcggcgct cgatttcgtc gatgaactgc
     2881 cgcgcaccac ggtcggcaag ctttcccgac acgaattgcg caagctgcag cccgcgcaag
     2941 cgaaacagca acaagtcgca tcaggaggtc gttagtaatc ctttcgtcgt cccggccaag
     3001 cgaagcgcga gccgggaccc atcacgccgc ggacgcgctt gaggcactag gttggacggc
     3061 tttgccactc atgaaggccg atggttatgg gtccccgcgt tcgcggggac gacgagaaga
     3121 gaaagatcgc gttaattcac tcgctcacag gaggatccga tggatctcag ctttaccagg
     3181 gaagaaatgg cgttccgtga cgaggtgcgg gccttcttca gggacaatgt gccgtccgcg
     3241 acgcggcaga aactgcagga gggccgtcat ctttcaaaac aggagatggt cgattggtgg
     3301 cgcatcctca acaagaaggg ctggggcgtg acgcactggc ctaaggaata cggcggcacc
     3361 ggctggagct cggtgcagca ttacatcttc aacgaggaat tgcagatgca tccggcgccg
     3421 gcgccgctcg ccttcggcgt cagcatggtc ggccccgtga tctacacctt cggcaatgag
     3481 cagcagaaga agtactatct gccgcgcatc gccaatgtcg acgactggtg gtgccagggc
     3541 ttttccgagc ccggctccgg ctccgacctc gcctcgctga agacaaaagc cgagcgcaag
     3601 ggcgacaaat atatcgtcaa cggccagaag acctggacca cgctcgccca gcacgccgac
     3661 atgatcttca acctgtgccg caccgaccct gcggcaaaga agcagtcggg catctccttc
     3721 atcctgatcg acatgaagtc caagggcatc acggtgcggc cgatccagac catcgacggc
     3781 ggccacgagg tcaacgaggt gttcttcgac gacgtcgagg tgccggtgga aaatcttgtc
     3841 ggtgaggaga acaagggctg ggactatgcc aaattcctgc tcggcaacga acgcaccggg
     3901 attgcccgcg tcggcgtctc caaggagcgg atccgccgca tcaaggaatt ggcctcgaag
     3961 gtcgagtccg gcggcaagcc ggtgatcgag gatcagaaat tccgcgaaaa gctcgccgag
     4021 gtcgagatcg agctgaaggc gctcgaactg acccagcttc gcgtcgtcgc cgacgagggc
     4081 aagcactgca agggcaagcc caacccggcg tcttcggtgc tcaaggtcaa gggttcggag
     4141 atccagcagg ccaccaccga gctgctgatg gaggtgatcg gcccgttcgc cgcgccctat
     4201 gacgtgcacg gcgacgacgg ctccaacgag gcgatggact ggaccgcgca gatcgcgccg
     4261 agctatttca acaaccgcaa ggtctcgatc tacggcggct ccaacgagat ccagcgcaac
     4321 atcatcacca aggccgtgct ggggctttag tgtcttctcc ctctgcccgc ccttcgcggg
     4381 gtcgagacga gcgaagctcg ctcttagagg gttggggtga ggggctcttc gatccgtaaa
     4441 taccgtggtt gcgttgagct caccgccctg cccctcaccg ggcgctacgc gccgacctct
     4501 ccccgtaaag gacggggaga ggttaagaca agagttcgtg ggatgggttg agcgaagcga
     4561 tacccatcat caacaacacc gaaacggtgc gatgggtttc gcaagcgctc aacccatcct
     4621 acaggttcga tggacaatgc ttcaggcgtg atttgagaga gtaagaaaca tggattttga
     4681 tttgtccgag gagcagcggc ttctcaagga aagcgtcgac ggcttgttgg gctcatccta
     4741 cgaattcgag cagcgcaaga aatacatggc ggagaagggc ggctggagca gaagcgcctg
     4801 gaacaagctc gccgaacagg gcctgctcgg cctgccgttc tcggaagacg acggcggctt
     4861 cggcgccggc gcggtcgaaa ccatgatcgt gatggaggcg ctgggcaagg cgctggtgct
     4921 ggagccttat ctggcaacgg tcgtgctcgg cggcggcctg ctgcgccgcg gcggctcggc
     4981 cgagcagaag gcggcgcaca ttccttccat cattgacggc agcaagaccc ttgcgttcgc
     5041 gcagctcgag aagaattcgc gctacgatct caacgacgtc tcgacctcgg ccaagaaaaa
     5101 gggatcgggt tacgtgatcg acggcgagaa attcgtcgtg ctcaacggcg agagcgccga
     5161 cacgctgatc gtgaccgcgc gcaccaaagg cggccagcgc gataccggcg gcatcggcgt
     5221 gtttctggta cccggcaacg ccggcggcgt cgcgaaaaag ggctatccga cccaggacgg
     5281 cctgcatgcc gccgatatct ccttcaccgg cgtcgaggtc ggcgccgacg ccgcgatcgg
     5341 cgatcccgac aacgcgttgc cgctgatcga gcgcgtggtc gacgacgccc gcatcgcgat
     5401 ctgcgccgaa gccgtaggca tcatggatga atcgctgaaa accacggtcg aatacctcaa
     5461 gacccgcacc cagttcggcg tgccgatcgg caccttccag accctgcagc accgcgccgc
     5521 cgacatgttc gtggccctgg agcaggcccg cagcatgtcg atgttcgcaa ccatggcttc
     5581 cgacttcgat gacgccaggg agcgcgccac cgcggtggcg gcggcgaaag tccagatcgg
     5641 caaatccgga aagttcatcg gccagcaatc gatccagctc catggcggca tcggcatgac
     5701 ccaggaagcc aggatcggcc actacttcaa gcggctgacc atgatcgaga acacctttgg
     5761 cgacaccgac taccacttgc gtcgcgtcac cgactcgggc gggctggttt agctccagcc
     5821 gtcattccgg ggcgcgagcg taagcgagcg agcccggaat ccataaccac catcgggcgt
     5881 atggattccg ggcctgcgcc aagaggcgca tcccggaatg acgagggaag cagagcgcca
     5941 acactctccg tctccgtcat tgcgagcgaa gcgaagcaat ccatggcccg gaaacgaaca
     6001 atggattgct tcgtcgctgc gctcctcgca atgacggcat caggtaattc agcgaggaat
     6061 caacaatgaa aaccaccccg ttcgatctca ccggaaaagt cgccgtcatc accggctcga
     6121 gccgcggcat cgggcgcgcc tcggctgaac tgctggcaaa gctcggcgcc agggtggtga
     6181 tttccagccg caaggcggac gcctgcgagg aagtcgccgc cggtatccgt aaggccggcg
     6241 gagacgccca cgtcatcccc tgcaacatct cgcgccgtga ggaagtcgag gcgctgacct
     6301 cgggcgcgac cagacactac ggcaggatcg acatcctggt ctgcaatgcg gcggtcaatc
     6361 cctattatgg cccgctgctc gacatcaccg acgaagcctt cgacaagatc atgggcagta
     6421 acgtcaagag caacatctgg ctgtgcgcgc tggcgatccc gccaatggcc gagcgcggca
     6481 atggttcggt ggtgatcatc tcctcgatcg gcggcctgcg cggctcgacc gtgatcggcg
     6541 cctacggaat ttcgaaagcc gcggatttcg cgctgtgccg cagcctcgcc ggcgaatggg
     6601 ggccgaaggg cgtgcgcgtc aactgcgtcg cgccgggcct cgtcaaaacc gatttcgccc
     6661 gcgcattgtg ggaagacgag gagcgcctga agcgccgctg cgccaccacg ccgctgcggc
     6721 ggatcggcga gcccgacgag atcgccggcg cggtcgccta tctctcctcc gacgccgcaa
     6781 gcttcatgac cggccagacc atcgtcgtcg acggcggcgt caccaccgcg tcggtgtgaa
     6841 ttcttctccc ctcctcccac gcgcatcgcg cgtggtgggg aggggtcggg ggtggggggc
     6901 ggctcggcac actcgctgct agctgcgccc gctgacagac cccccacccc cgacccctcc
     6961 ccaccgctgc gcggggggag gggagcaaca cgtcatcata ttgaccttcg ctccccaatc
     7021 cgcttgcctt gccgcaacac cgcaaaaaca tcccgggaag caacgccagg aaaaacatca
     7081 tgtcccatgt gctggccatc gatcagggca ccacatcctc gcgcgccatc gtgtttcgct
     7141 ccgacatttc catcgccgca tcagcgcaac aagaattccc gcagcacttt ccggcctcgg
     7201 gctgggtcga gcacgagccc gaggacatct ggatctcgac gatcgccacc tgccgcgagg
     7261 cgctgaagaa ggcgggcgtc ggcgcaaaag acatcgccgc gatcggcatc accaaccagc
     7321 gcgagaccgc cgtggtgtgg gaccgcgcca ccggccaggc ggtgcaccgc gccatcgtct
     7381 ggcaggaccg ccgcactgcc gatatctgcg ccagattaaa ggccgaaggc cacgagagcg
     7441 cgatcacgga aaaaaccggt ctgatcatcg atccctattt ttccggcacc aaggtcgcct
     7501 ggattctgga ttccgtgccc ggcgcgcgcg agcgcgccgg gcgcggcgaa ctgatgttcg
     7561 gcaccgtcga ttgctatctc ttgtggcggc tgaccggcgg caaggtgcac gccaccgacg
     7621 ccaccaatgc ctcgcgcacg ctgctgttca acatccatac cgggcaatgg gacgacgatc
     7681 tcctgaaaat cctgcgcgtg ccgggctcga tgctgccgga ggtgaaggat tcctcgacca
     7741 aattcggcga cagcgtaccc gaactgttcg gcggcgcgat cccgatctcc ggcatcgccg
     7801 gcgatcagca ggccgccacc atcgggcagg cgtgttttgc gcccggcatg atcaagtcga
     7861 cctacggcac cggctgcttt gctttgctca acaccggcac gacgccggtt gcctcgaaga
     7921 acaggctctt gaccacgatc gcctatcagc tcaagggcca gcgcacctat gccctcgaag
     7981 gctcgatctt cgtcgcaggc tcctcggtac agtggctgcg cgacgggctt ggaatcatca
     8041 agcaggcttc cgagaccggg gcgctcgccg accagtccga ctccacgcaa tcggtctatc
     8101 tggtgccggc ctttgtcggc ctgggcgcgc cgtactggaa tccgcgcgtg cgcggcgcgc
     8161 tgttcgggct gacccgcaac accggccccg ccgagctcgc ccacgcggcg ctcgaaagcg
     8221 tctgctacca gacattcgat ttgcgcgccg cgatgcgcgc cgactggccg gaagccaagg
     8281 ccgcaaacgt gctgcgcgtc gacggcggca tgaccgcttc cgactggacc atgcagcgcc
     8341 tcgccgatct cctggacgcc ccggtcgatc gccccatgat ccaggaaacc accgcgctgg
     8401 gcgccgccta tctcgccggc ctcgccgccg gcgtctatcc ggagcccgaa aaattcgccg
     8461 acaactggcg gctggagcat cgcttcaagc caaacatgag cgcggccacg cgcgagcgga
     8521 agctcaaggg ctgggcgcgg gcggtgaagg gcttgctcgc gagcgatgag ggcgaggggt
     8581 agctctcctc cctctccccg cacttcgcgg ggtcgagacg agcgaagctc gctcttagag
     8641 ggtcggggtg aggggctgtt tccgtgcgtt cagtcgtcat cgcccgcaga aatgtttggg
     8701 tcgagcgctg ggatttagta tggagcccat gggtccgtta cgatgacgtc ataggccgga
     8761 taccaatagc agtactcgtc gatgccgtcg tcaaagcagt atccataata aaaatccgcc
     8821 tgtgcgagtt gaattggacc atttgctgca attttcacga taccggtgtc tccactcgcc
     8881 agctttttgt tcgacttgac ttttcgcgcc ggcaggctgc ctgcgctcat tccaaccacc
     8941 ttgtcgttgt tcacttctgc gacgaccgcg ttgccgccta tttttccgac ttcgtgcttt
     9001 ccatttttat ggagcttggg tccaagaagg ttatgaccgt tattgtgatg gggatttttg
     9061 gccagcgcga atccggcggt cactagcacg ctcgtgcaaa cgacgagttt cacgatggtc
     9121 cggcgattga acatctatgc ttctccactt catccgattc acatgaaccg tgcttcgatg
     9181 cgaaccattc cgtttgacaa tttgttggcc acaacgggat tacagtgggg atctgttggc
     9241 tatttagagg taattgacag gcatttgatg atgatcgctt ccgactgacg aatccaggac
     9301 agggggggcc gcattcaaag atgcgccgac agattatcgc ccaacagctc gaagccgcct
     9361 tgcgcaactc gctgcaggaa tcgggtctcc gcctggtcag cctggacggc gagcggccgt
     9421 cggtcgatac aaggcgcgag ccctggatcg agatcaaata tggtccggcc agcccggacg
     9481 actggtttgg aatcgtgccg gcgacggcgc agttcgaacg gtccgcaggc aaaacccgtg
     9541 aagccttgaa tctgatcgtg aaggtcaacc cgcgcctcgg tttggcgcgc aatctcattc
     9601 cctggatcgt cgaaaacaaa tcgatccggc ttgaccggcc ttactgggag tatcgcacct
     9661 ccgcggagac cgaccgcacc gcggatcgcg aatttcacat ctacgaatta gctgcaacag
     9721 gcagctctgc cctgtcctgg gtcttgccgc gctattacgg cgctgcgagt gaccccgcga
     9781 ccggcgaacg ggctttgttc atggaacggc tcacggacgt ctcgcgcctg gatgcagccg
     9841 gcgtgcaggc cgactggccg atcgaggcga tcggcgccgc gttcgcggcg gcggcggcat
     9901 ggcaggcgga attctggggc gcgggtaatg cttccctggc atgggccggg ccgcgcccta
     9961 cgactgacga catgctggcc gacgagtcgt tatggcgcgg gctactggat gacgcccatg
    10021 cgcgatttcc agccgtcatc acggaagagg ttagaagccg gcggcaccgg ctgatcgata
    10081 cgatggatgt atggcatcgc gccaaggacc tgttgcccgg caccctggcg catgacgatt
    10141 tcaacaaccg caatgttgga ttccggcccg gcgttgtcgc gctagattgg gaactggcgc
    10201 aatgcaacac cgcgcatcgc gatctggtcg agatgctgac attcgtcgcg ccgtcggcgg
    10261 cgacccgcgc ggatatcgat cggctgatgg agcgtcaccg gaccatgctc gccgaaacgg
    10321 gggtcaaggc gggactggat cgcgacacgt ggatggaggc gtttcgctgc gaattgaagg
    10381 tcgaggcgat caaccgcatc agtatgcagt gtattttcga ggcggcgttc ccgctggcct
    10441 atttcgagcg catcaacaca aatatcgagc ggctgcttga tctctacggc tagggggcct
    10501 cttggcaagc ggtgagggga agggttagga ctctctcagc cgtcatcacc gggcttgttg
    10561 ttcagcccgg ggatccacgt cttacttttc tttttcagga gacaaagccg tggatggccg
    10621 ggtcaagccc cggccatgac gagtggagat gacaaggtgc acgcccatga tcctgatcgg
    10681 ccaatacgac tccccgttcg tgcgccgcgt cgcgattgcg ctgcggcttt atgagttgtc
    10741 gttcgagcac cggccgtggt cgacctttgg cgatggcgaa aaaatcgcgc cgtataatcc
    10801 gctgcggcgg gtgccgacgc tggtgctcga tggcggcgag gcgctgatcg agagcacggc
    10861 gatcctggat tatctcgatg aggtggccgg tccggacaag gcgatgatcg cggcgcgcgg
    10921 gccggagcgc cgccgggcct tgaaaatctg cgcgctggcg agcgggcttg gcgacaaggg
    10981 cgtcagcctg ctctacgagc gcgtgctgcg gaaggatcaa tcgaaaatct gggtggagcg
    11041 ctgcgaggcg cagattttca gcgtgctcga tgtgctggag caggagcgca cggcggtgaa
    11101 gacgccatac tggttcggcg agcgcatcgg gcacgccgac gtcgcggtcg cctgcgcgct
    11161 gcgcttcacg tcagaggcgc atccgcaact gttcgacgcc gcgcgccatc cggcattggc
    11221 cgcgcacgcc gcgcagtgcg aagcgctgcc gccgttcaag gaaatcgtgc agccgctggc
    11281 gccgccgggc gccgactaag tcgcgaactc atgaaagcgc cccggacgga cgatttggtg
    11341 cccgccatgc gccgaaggcg acaaacatcc atttaagggg cccccagagc tgcgccaggc
    11401 ggaccgatcc atttctttgc gcagcttttt cagcatatac gcgggttatc gccgacgtcg
    11461 caattttccg cttccggctg cgccggtgga aatgcgccga cgcgcggcgc tgaaatgcc
//
LOCUS       DATNOI010000099         2468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1894660,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000099 DATNOI010000000
VERSION     DATNOI010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894660"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VN968_03445"
     CDS             complement(<1..563)
                     /locus_tag="VN968_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HXL28332.1"
                     /translation="MHGLLTTLGRGFKKRIGWKRLGIVASLLIIAFAITTLVRTLKGV
                     DTGVVLIALTEIPPDRIALAALCVVGAFCTLTFYDFFALRTIGKKHVPYRIAALSSFT
                     SYTIGHNIGATVFTGGAIRFRIYSDYGLTAIDVAKICFLSGLTFWLGNLVVLGFGMAL
                     HPWAASAMDQLPPAINRLIALGGLAAIV"
     gene            876..1148
                     /locus_tag="VN968_03450"
                     /pseudo
     CDS             876..1148
                     /locus_tag="VN968_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
     gene            complement(1217..1651)
                     /locus_tag="VN968_03455"
     CDS             complement(1217..1651)
                     /locus_tag="VN968_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28333.1"
                     /translation="MAETTYFPRRLILAGALISGVLLALAVHMLGARYGLDLGGLWRS
                     DTREFMPLGAAVAWWLIATVGFSGGYFTATLMHSAVSGQIPRRMRQFLIAVGVLVLAG
                     AGQAASAPSPVPTISGVLAGLAALGLGAGMAFCGAHFALRKA"
     gene            1734..2168
                     /locus_tag="VN968_03460"
     CDS             1734..2168
                     /locus_tag="VN968_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HXL28334.1"
                     /translation="MAFGLVALISAAIFTGAALYVNVAEQPARLLLDDRALLTEWKPS
                     YRRGAAMQAPLALLGCLLGLIAWWQTSQPGFLVGAIAMIAPWPWTLIGIKPTNDALLA
                     AEPDQSGPQIRALVVKWGVLHSVRTALGALATLAFLWACIPR"
     gene            2230..>2468
                     /locus_tag="VN968_03465"
     CDS             2230..>2468
                     /locus_tag="VN968_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28335.1"
                     /translation="MTGLANEAAADIRYAYKASLIGAAHQFELTGEGLSWRIGGKAGL
                     WPYRDIAAIRLSYRPVSMQSRRFRADIEHANRQRIA"
BASE COUNT          500 a          759 c          784 g          425 t
ORIGIN      
        1 acgatcgcgg cgaggccgcc cagggcgatc agccggttga tggccggcgg cagctgatcc
       61 atcgcggagg ctgcccacgg gtgcaacgcc atgccgaagc cgagcacgac caggttgccg
      121 agccagaagg tgaggccgga aagaaagcag atcttggcga cgtcgatggc ggtcagcccg
      181 tagtccgaat agatccggaa gcggatcgcg ccgccggtga acacggtagc gccgatgttg
      241 tggccgatcg tgtagctggt gaagcttgac agcgccgcga tgcgataagg aacgtgtttc
      301 ttgccgatgg tccgcagcgc gaagaaatcg tagaacgtca gcgtacagaa ggcgccgacc
      361 acacacagcg cggcgagggc gatgcggtcg ggcggtattt cggtcagcgc aatcaggacc
      421 acgccggtgt cgacgccctt gagggtacgg accagagtcg tgattgcgaa agcgatgata
      481 agcaggctcg ccacgatccc gagccgtttc cagccgatcc gtttcttgaa gccacgcccc
      541 agcgtagtca gcagtccgtg cattcatcct cccggcaggg cggttacaag ttgcaagcct
      601 tttcgagcga tcgaggcaag cgacggccaa ttgttcaacc tgcgatgacc cataaggcaa
      661 aaacgccgcg ttgtgcagtc cttgacaagg gcggttaaag ctcttccgca ggcgcggctg
      721 tcacactcct tacatatgtc gcaatgcagg agattcgagc cctccatttt acctagcggg
      781 cccaaccgtt catctacggc gatgttccga tgttccatga agacgttttc attgtccgca
      841 ggaacaacct cgcggtcaca cggttagcgc cgggctgtcc ggccatcgac ggcatcatca
      901 agcaggcaga cgagaccgcc ggcgaggtcg cggataaagc ggtgctggat gcggcattga
      961 tcaatgccgc gcaggccgcc gaacactacg agatcgcgcg ctacggcagc ctgatcgcgt
     1021 gggcgaagca gcttggccgc aacgattgcg ccagcgtctt gcagaagacg cttgaagaag
     1081 aaaaggcaac cgacaagaag ttgaccacgc tggcggagag caagatcaat ctccgcgcgg
     1141 cgagctgatc ttccaagaga tcattgagac aaaacgccgc gctggctcgt ccgcgcggcg
     1201 ttttgcgttg tgagcgttac gccttgcgca gcgcaaaatg tgcgccgcag aaggccatcc
     1261 ctgcgcccag gcccagcgcc gccaaacctg cgagcacgcc cgatatcgtc gggaccggac
     1321 tgggcgcaga cgccgcctgg ccggcgcccg ccagcacgag cacgccgacc gcaatcagaa
     1381 attgccgcat ccgccgcgga atttgccccg ataccgcgct gtgcataagc gtcgcggtga
     1441 aatagccccc cgaaaagccc accgtcgcaa tcagccacca cgcaacggct gctcccaacg
     1501 gcatgaattc gcgtgtatcg gatcgccata gaccgccgag atcgagcccg tagcgcgcgc
     1561 ccagcatgtg cacggcgagc gccagcagca ctcccgaaat caacgcgccg gcgaggatca
     1621 ggcggcgtgg aaaataggtc gtctcggcca tgctccgctt gtaggataaa ggcggcagtg
     1681 cgcccaagct ggcgccacga attcgatcga tgtttccaaa accggctgga gccatggcct
     1741 tcggacttgt tgcgttgatc agcgcggcta ttttcaccgg cgcggcgctt tacgtgaacg
     1801 tcgccgagca accggcgcgg ctgttgctgg atgatcgcgc gctgctaacc gaatggaagc
     1861 cttcctacag gcgcggagcc gcgatgcagg cgccgctcgc gctgctgggt tgcctgctcg
     1921 gattgatcgc gtggtggcag acctcacaac ccggattttt ggtcggcgcc attgcgatga
     1981 ttgcgccgtg gccatggacg ctgatcggga tcaagcccac caacgatgcg ctgctggcgg
     2041 cggagcccga tcaatcgggt ccgcaaatac gggcgcttgt cgtcaaatgg ggcgtattgc
     2101 acagcgtgcg caccgcactg ggcgcgctcg caacgctggc ctttttatgg gcatgtatac
     2161 cgcgctgagc gcggcgtacc gattgccgag cgcctgcgtc gatctcgcga gaatagcgga
     2221 caagctataa tgactggttt ggcgaacgag gctgcagctg atatccgcta cgcctacaag
     2281 gcctcgctga tcggcgcggc gcatcagttc gaactgacgg gcgagggctt gtcgtggcgg
     2341 atcggcggca aggccgggct ctggccctat cgtgatatcg cggcgatccg tctgtcatat
     2401 cgcccggtat cgatgcagtc gcgccgcttt cgcgccgata tcgaacatgc gaaccgtcag
     2461 cgcatcgc
//
LOCUS       DATNOI010000100         2006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_663982,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000100 DATNOI010000000
VERSION     DATNOI010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663982"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VN968_03470"
     CDS             <1..1013
                     /locus_tag="VN968_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HXL28336.1"
                     /translation="GSEQIGTLITALGTGIGRDDFDLSKLRYHKIILMTDADVDGAHI
                     RTLLLTFFFRQMPALIEGGYLYIAQPPLYKVNRGKSEQYLKDERALEDYLIATGLDDC
                     VFKTASGEDRSGRDLQALVEDARIIRGTLHNLHSRYNRKVIEQAAIAGVLSPKITGNV
                     ETANAAAGYIARRLDALADEVERGWTGRFTEGEGFFFERTVRGVKDVAIIDDALLGSA
                     DARKLDEYAAKLQEAYPRPGLLRRKDTKTTIHGPVGLFEAVTGAGREGIKLQRYKGLG
                     EMNPGQLWETTLDTNERSLLQVKIKEVDEADDIFTKLMGDVVEPRREFIQDNSLTANV
                     DV"
     gene            1156..>2006
                     /locus_tag="VN968_03475"
     CDS             1156..>2006
                     /locus_tag="VN968_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXL28337.1"
                     /translation="MTSSDNIERASIAAPAGAAGRHLAVELTETLKLAVPMALTQLGQ
                     IAMMTTDLALIGRLGDEAVAAAALAHTVFFVSFTFGMGLMSAVAPLAAQAFGARNPRL
                     VRRSLRVGLWAGLLISLPMMAFPLFGEVILVMLGQAPATAHLAQQYLFGLAWGITPAL
                     WFLAIRGFMGAVNRPEPGLWITLAAIPANAVLVYLLIHGELGLPRLELFGAGLGSTII
                     DFGMLLAALWIAYGRRPFRKYRVLGHIWRIDWTLLRQLIVIGAPISIAFLLEYGLFAA
                     AALLMGL"
BASE COUNT          351 a          639 c          639 g          377 t
ORIGIN      
        1 tgggctccga gcagatcggc acgctgatca ctgcattagg caccggcatc ggccgcgacg
       61 attttgattt gtcaaagctg cgctaccaca agatcatcct gatgaccgac gccgatgtcg
      121 acggcgcgca tatccgcacg ctgctgctga cgttcttctt ccgccagatg ccggcgctga
      181 ttgagggcgg ctacctctac atcgcgcagc cgccgcttta caaagtgaac cgcggcaaat
      241 ccgagcaata cctcaaggac gagcgcgcgc tcgaggatta cctgatcgca accggtctcg
      301 atgactgcgt cttcaagacc gcgtccggcg aggatcgcag cggccgcgac ctgcaggcgc
      361 tggtggagga cgcccgaatc atccgcggca ccctgcacaa tctgcacagc cgctataacc
      421 gcaaggtgat cgagcaggcg gcgatcgccg gcgtgctcag tccgaagatc accggcaatg
      481 tcgagactgc caacgcggcc gcgggctata tcgcgcgacg gctcgatgcg ttggccgatg
      541 aagtcgagcg cggctggacc gggcgcttca ccgaaggcga gggtttcttt ttcgagcgca
      601 ccgtgcgcgg cgtcaaggat gtcgccatca tcgacgacgc gctgctcggc tccgccgacg
      661 cgcgcaagct cgatgagtat gccgcaaaac tgcaggaagc ctatccgcgc cccggcctgt
      721 tgcgccggaa agacacaaaa accaccattc acggcccggt gggcctgttc gaagcggtga
      781 ccggagccgg ccgcgagggc ataaagctgc agcgctacaa aggcctcggc gagatgaacc
      841 cgggccagct ctgggaaacc acactcgata ccaacgagcg ctcgctgttg caggtgaaga
      901 tcaaggaggt cgacgaggcc gacgacatct tcaccaagct gatgggcgac gtggtcgaac
      961 cacgccgcga attcatccag gacaattcgc tcaccgcgaa tgtggatgtg tgattaatcc
     1021 cgccgcgtag ctgcgtaggg tgggcaaagg cgccgttgtg cgccgtgccc accattggcc
     1081 gcacattaca gccgactgag cgattggatg gtgggcacgc ttcgctttgc ccaccctacg
     1141 cggtagtgtc gctccatgac atcgtcagat aacatcgagc gagcatcgat tgccgcgcct
     1201 gctggcgcag caggccgcca cctcgccgtc gagttgaccg agacgctcaa gctggcggtg
     1261 ccgatggcgc tgacgcagct cgggcagatc gcgatgatga cgaccgatct tgcgctgatc
     1321 ggacgcctcg gagatgaggc ggtcgccgcg gcggcgctgg cgcatacggt gttcttcgtc
     1381 agcttcacct tcggaatggg cctgatgtcc gccgtggcac cgctggccgc gcaagcattc
     1441 ggcgcccgca acccgcgcct ggtccggcgc tcgcttcgcg tcggtctttg ggcgggcttg
     1501 ctgatttccc tgccgatgat ggcgtttccg cttttcggcg aagtgatcct tgtgatgctc
     1561 ggccaggcgc ccgcgaccgc gcatctcgcg caacaatatc tgttcggcct ggcgtggggc
     1621 atcacgccgg cactgtggtt cctggccatc cgcgggttca tgggcgcggt caaccggccg
     1681 gagccgggac tgtggatcac gctggccgcg atcccggcca atgccgtgct ggtctatctc
     1741 ttgatccacg gcgaactggg attgccgcgg ctggagctgt tcggcgccgg tctcggctcc
     1801 accattatcg atttcggcat gttgctggcg gcgctatgga ttgcctatgg ccgtcggcca
     1861 ttcaggaaat atcgcgtact cggacacatc tggcgcatcg actggacgct gctgcggcaa
     1921 ctgatcgtga tcggcgctcc gatctccatc gcgttcctgc tggaatatgg cctgttcgcc
     1981 gcggcggcgc ttttgatggg cctgat
//
LOCUS       DATNOI010000101        18091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_824868,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000101 DATNOI010000000
VERSION     DATNOI010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18091
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824868"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1632)
                     /locus_tag="VN968_03480"
     CDS             complement(28..1632)
                     /locus_tag="VN968_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXL28338.1"
                     /translation="MPLHSSTDPTSSDFAGNAEAMRLLVAELREKLDQVAGGGGETSR
                     ARHTSRGKMLARQRVDLLVDPGTAFLELSPLAAYGLYGGDVHSASVITGVGRISGREC
                     VIVANDATIKGGTYYPMTVKKHLRAQDIARQNNLPCVYMVDSGGAFLPQQDEIFPDER
                     HFGRIFYNQAQMSAAGIPQIAIVMGSCTAGGAYVPAMSDESIIVRNQGTIFLGGPPLV
                     KAATGEVVTAEELGGADVHSRQSGVTDHYAQNDSHAIGIARRIVATLKQPARANLNMR
                     EPREPLFAREEIYGVVPADGRKPYDVRDIIARVVDGSEFDEFKKLYGQTLICGFAHIW
                     GYPVGIIANNGILFSESALKGAHFIELCCQRNIPLVFLQNITGFMVGKKYEAGGIARD
                     GAKLVTAVATAAVPKFTVVIGGSYGAGNYGMCGRAYSPRFLWMWPNARISVMGGEQAA
                     MVLSQLRRDNIEAKGGSWSIEEENEFRAPTRAQFEGQGNPYYATARLWDDGVIDPADT
                     RLVLGLGLSAASNAPIEPTRFGLFRM"
     gene            complement(1773..2078)
                     /locus_tag="VN968_03485"
     CDS             complement(1773..2078)
                     /locus_tag="VN968_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HXL28339.1"
                     /translation="MTARIFKPAKNAMQSGTAKTREWQLDYEPEQPQAIEPLMGWTSS
                     GDMKQQLTLRFDSKEEAVAYCEREGIPYQVSEPKEPARRQAAYADNFAFRRAEPWTH"
     gene            2307..3461
                     /locus_tag="VN968_03490"
     CDS             2307..3461
                     /locus_tag="VN968_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HXL28340.1"
                     /translation="MKINATLPEKGRDLRLDLFRGVANWAIFLDHIPDNVVNWITTRN
                     YGFSDAADLFVFISGYTASFVYARMMIERGFIVGATRLTKRVWQLYVAHVILFVIYIV
                     SIGYVAQRYSDPDIINEFNVAGLVDNPIETLRQGLLLKFKPVNLDVLPLYIALMGLFP
                     PVLWMMLRQPNLTMLASIVLWLAARQFGWNLPAYPAGAWYFNPYCWQVLFVFGSWCAL
                     GGARKSMSFINAPMTLYFCLAYLVLALVMTMAGKFPDFGALFPHWLYETFNPNDKTNL
                     APYRFVHFVVIVILVIRFVPKNWPGLEWRIFDPVIVCGQQSLAVFCVGVFLSFVGHFE
                     LMMSSGSLFAQIFVSVTGIAIMTIVAYYISWSKRQDKPLPKPAAAPAKAG"
     gene            complement(3570..4415)
                     /locus_tag="VN968_03495"
     CDS             complement(3570..4415)
                     /locus_tag="VN968_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL28341.1"
                     /translation="MVDYHGRFAWYELMTTDMAAAKAFYAAVVGWGTQDASTPELAYT
                     LFTTGAASVSGLMDLPEEARKMGATPRWMGYIGVDDAGVTSDRIKRLGGAVYVPPTNT
                     NIGRISVVADPQTATLALIEGPKKPGRQQPAELDRPGHVGWHELLATNWEKAFAFYGE
                     LFDWQKANTEIAPTNTYQLFAAGGQTIGGMFTKRPVEPVPYWLYYFNVDDIDAASERV
                     KTGGGQIFEGPIEVPGGNWMARCIDPQGAIFALQGKRSQHGFGWSTEWDGFSSKGRLV
                     TKPRA"
     gene            5438..7696
                     /locus_tag="VN968_03500"
     CDS             5438..7696
                     /locus_tag="VN968_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HXL28342.1"
                     /translation="MTGHTKTPSKQHTGKEVLQFAPNFTAYVLPPDVVCLYSEDRKFF
                     LHGELYCALASAIGKNGRSLRELVGRLEQDFPSDKINEALKRLLERRYVVSVTNSSAS
                     TVAGYWSSLGLPPGMAETNLENCRVRVQSIDVQGAAELDAALRELGVRVVKSSPDLTV
                     TLVNDYLERQLAELNLQRVSDKTPWLLVQPSGAFPLVGPVFIPGESACWTCLFDRMIR
                     NREVKGFLDRGPAHPVAVSPLVRNAVGQGGIQLAALEIAKAIATGFRTELRDHIVSLD
                     LPGATIAKHYVAARPQCPTCGRKKLRDPRRAPVPIEPGPGAKLVMTSGGYRTVSSRTT
                     VARFRKHVSPLTGVVTRLERIEADLPMNTNFFAQHNFSAPARNVDELRSGLSGGSFGK
                     GSTAEQGEASALMEAIERYSGIYQSDEIRLTRRFADFAPGDAILPNDVLLFSEAQSQP
                     DETQTDDSHHQMVPARFDQSAKIEWSPVWSLRDQRFRYLPTSILYFFYSGPAAFHADS
                     NGCAAGNTLEEAIVQGFLELVERDSYAIWWYNRVQRPEVDLSQFDDSYVRDLQTQLAE
                     TGRRVWVLDITSDLGVPTYVAILHWMQNGQENIEFGSGAHFDKRIALLRALTELNQFL
                     SIGLMGGGTGEKSSLDGITPLRLEDHPFLKPGGNPTAQSEDDLKVPLDNTRGQVDACV
                     NIAKRAGLDFLVLDQTRPDVEVPVVRVIVPGLRHFYLRFAPGRLYDVPVKLGWLDRPL
                     PESELTPFLPHT"
     gene            7709..9181
                     /locus_tag="VN968_03505"
     CDS             7709..9181
                     /locus_tag="VN968_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HXL28343.1"
                     /translation="MRAPGKKRGRKIAPSVVSAWLSGHITLEAQADGNIVACFDGYSV
                     GLGQFSAGAVARAQDLRTGLSLASFASDGRTADKEIDLLVQRLARRGLLEYRLGPSGD
                     GNALVVIEPQIPDYWPRTPKLGDSEIIVLSRFAYLRRRGHEMVLESPRAGALFRICDP
                     NIASALAALSTPQRISRLRRQDNFPGIGLLALLVDCQILFRIDAANDQGLRPTEGDDN
                     LVLWDFHDLLFHTHSTEGRHANPLGGLYPHAGAIAPLPAVRPRWPGKKIDLQKFSAEP
                     SRLNSPVAKLLLERHSMRDFDDQQPITLAELAQLLDSTARVRSSWKSGLDFGEGGPEI
                     EYAARPYPAGGSAYELELYLTVANCEGLARGFYHYDAGSHALVPIAVGAPELEATLAA
                     AEFAMDAPAPPQILITIAARFGRTSWKYTSIAYSLILKDVGVLIQTLYLMATDMGLGG
                     CAIGTSHIELFAKMTGIEFHVEGPVGQFAIGRGRNLEASD"
     gene            complement(9199..11007)
                     /locus_tag="VN968_03510"
     CDS             complement(9199..11007)
                     /locus_tag="VN968_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HXL28344.1"
                     /translation="MSKQHTYVLGLNTYDHDVSACLLRDGAIAFAIAKERITREKHAT
                     GFYKEVIDYCLDAEGITLDDVDLIVRNCYILPVAEMEERLVYQDMPGFLPEYERAEAS
                     RHPLYLSGSDRVVSISHHLAHAYSAFAASPFEDGVVMIVDGVGSYQSDVVEPYPSTDT
                     ATPLARESESYYTFSGARIECLKKVWMEPDRGFLSGEFYNLPGLGALYSRASTYIFGD
                     WNKCGELMGLAPYGRCEQVKHLLELSDGTLHVPRWTTDFRQPYVMDGGSWEVSPSMRH
                     WEDLAWRVQDDTENVLLARARWLRETTGAKNLCIAGGVALNCVANGRIVREAGFENVW
                     IQPAAGDDGIAIGCAYYGYLEILAQRRTFVMDHAYVGKRYTDQDIDNATGKFLVRIQT
                     SAVRCENICRETAQLLVDQRVIGWFQGGSEFGPRALGNRSLLADPRKGEMKDILNKRV
                     KHRQAFRPFAPIVLAERAREVFEGEEESPFMLVAKNVRPEWRDRIPAIVHVDGTARVQ
                     TVREQTNPMLYRLLREFEALTGIPVLINTSFNVKGEPIVETPRDAVICFLTTGIDNLV
                     LHDRLVSKNAAHKIVGPLVGIYADVATMVMAGTRSA"
     gene            complement(11123..11443)
                     /locus_tag="VN968_03515"
     CDS             complement(11123..11443)
                     /locus_tag="VN968_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28345.1"
                     /translation="MGRYICLNHQNLDLARHNFQDQAFVPARGNIVYNMVFLIVFVGL
                     FCDAIEKGERAQFTDFDRRKSKNGSNIDARADIVALSGDFFDQRTNQICQNCDLAKCD
                     AAKL"
     gene            12013..12171
                     /locus_tag="VN968_03520"
     CDS             12013..12171
                     /locus_tag="VN968_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28346.1"
                     /translation="MAAGGVVLPALYLPVINMSKAIIRLSIPIGTIAISSVAIFAFSW
                     VGLALLGY"
     gene            complement(12235..12495)
                     /locus_tag="VN968_03525"
     CDS             complement(12235..12495)
                     /locus_tag="VN968_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28347.1"
                     /translation="MLGAPTAPIAWDGLGSPPTETADVPENRPKKTTRPKKEIIVGPI
                     GDVSSAGRNPKSQADEAFEDQEAADRAAETKLTRQLKICSNC"
     gene            complement(12786..13724)
                     /locus_tag="VN968_03530"
     CDS             complement(12786..13724)
                     /locus_tag="VN968_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HXL28348.1"
                     /translation="MSRRHEISSSPENMVWGYIDSATPPVLEIASGDTVTLHSFPAGG
                     KECLPEDRSLVPADYLAALDTLVQGPGPHFITGPVYVKGAKPGDVLQIDILKVKVRQD
                     WGFVSILPLLGTLPDEFTEYETIHPKVDHERNVCVMPWGLEIPLDPFFGIIATAPPPA
                     WGRCGSPVPRSFGGNMDNKELRPGTTLYLPIFNEGALFFAGDGHGVQGDGEVCITALE
                     TGVTGTFRLTVRKDMDLSWPFAESATHLMSIGLDEDLDDAAKQAVREMVKHICERTNL
                     SRNQAYMLCSLAGNLRVTQLVDGNKGIHMLLPKAYL"
     gene            complement(13755..14447)
                     /gene="urtE"
                     /locus_tag="VN968_03535"
     CDS             complement(13755..14447)
                     /gene="urtE"
                     /locus_tag="VN968_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HXL28349.1"
                     /translation="MRFRVESLDQHYGSAQVLRQVGFEIMPGECLAVLGRNGAGKTTL
                     LKCLMGVLPVTRGRILVDETEISGWSSHRRSVAGLAYVPQGREIFSELTVGENIEAAA
                     RAHGTFGTAIPEETIALFPVLKQMWRRPGGALSGGQQQQLAIARALVTRPSLLILDEP
                     TEGIQPNLVATIKDVLASFKGKISLLLVEQYLDFAISLADAFVVLSRGSVAEAGRTES
                     SSRELLTRYIAI"
     gene            complement(14450..15178)
                     /locus_tag="VN968_03540"
     CDS             complement(14450..15178)
                     /locus_tag="VN968_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL28350.1"
                     /translation="MSGVALLVDTVTVEFGSFTAIDMLSLAIDFGEVRAVIGPNGAGK
                     TTLLDVISGITRPKQGRVIFDDLTDIVRSSESAIARAGVRRKFQKPSIFEGLNVRQHI
                     AIGAEGGLRRSRSREKLDDRIGEVLEAVGLAGLANRSASELAHGQKQWLEIGMVLASN
                     PKILMLDEPVAGLTDEETERTVSLVRSLKRADRAIVVVEHDMDFVERIADRVTVLHEG
                     KTLFEGSMANARADERVIDVYLGR"
     gene            complement(15178..16269)
                     /gene="urtC"
                     /locus_tag="VN968_03545"
     CDS             complement(15178..16269)
                     /gene="urtC"
                     /locus_tag="VN968_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HXL28351.1"
                     /translation="MRSLGSDRPFLILLAVVLAVALLLGISIAIPFGLSAYLANAIGQ
                     LATFAVLALSLDLIWGYLGILSLGHGLFFGIGGYVVAMHLLKHSYEVTGKVPDFMQFM
                     GWKEFPFYWAGFGYSSYAIVVAAVVTLLIAGVFGYVSFRSRVGGVYFAIITQALVYVA
                     MLLMFRNDTGFGGNNGMTGFTVMFGWPIGLHGVVIAMAAISVLMLGLTLLGCRLLLES
                     RFGVLMIATRDDEVRLRTLGYDTLLLKLVIWCLSALIAALAGMLYVPQVGIINPRLLS
                     PDLSLEIAVWVAIGGRGHLIGAVIGAVLVNAVKFWLSAAAPELWPFILSGLIVLVALV
                     FPNGLVDLAKFKFVRPMISKRLTETPELR"
     gene            complement(16266..17885)
                     /gene="urtB"
                     /locus_tag="VN968_03550"
     CDS             complement(16266..17885)
                     /gene="urtB"
                     /locus_tag="VN968_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HXL28352.1"
                     /translation="MMRRHCIAAVCFIAAFASTHLWAAGAEQRVDLIGKVCGDATAMG
                     EAGRSLTRIAADGSPDDRSWAALIARALLDRKLTCDATGAVTASAVGSLDASTGLQRN
                     IGEGSRSPLLSLKNRALFETAEAALALRSAPDPARRSAALRTLERQASNLPEQLFDAA
                     VREEADPGLKTQLSAVAQAAALNSQDPAKRIAALGRIAATPDRRALTLVRPLVNDPAY
                     AANPDFKRAVDAATARIDRGIAVGDVLATLYNGLSFASILFMAAIGLAVIFGLMGVIN
                     LAQGELIMLGAYVTWLVQESLRHLAPGLLDWYLIIAIPFVFLITAAIGIGLEAVLLRH
                     LYRRPLMSLLATWAVSLFLINLVRVSFGTQNLQFVTPFYVTGGVPVIGDFIFTWNRMF
                     AIAFATVTLALTWALMQMTPLGLNIRAVTQNRTMAGCIGIPVRRVDMLAFGLGSGLAG
                     LAGLALAPIYSVNPQMGQNFIIDSFMVVVLGGVGTIAGTIVAALGIGQVNVLIEPLWG
                     AVAAKVIVLLMIIGFLQFRPEGLFAIKGRRK"
     gene            complement(17945..>18091)
                     /locus_tag="VN968_03555"
     CDS             complement(17945..>18091)
                     /locus_tag="VN968_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HXL28353.1"
                     /translation="SGYKLEMTAENNYVSRGVFIGSVNEKQGFDILWQSKDAPKPVPF
                     SPYS"
BASE COUNT         3525 a         5623 c         5427 g         3516 t
ORIGIN      
        1 gccggtactg tttggaccga tccatcgtca catcctgaac aggccaaaac gagtgggctc
       61 gatcggcgca ttgcttgctg ccgaaagacc aaggcccagc accagccggg tatcggcggg
      121 atcgatcacg ccgtcatccc acagccgggc ggtggcgtaa tacggattgc cctggccctc
      181 gaactgcgcc cgcgtcggcg cgcgaaactc gttttcctct tcgatcgacc agctgccgcc
      241 cttggcttcg atgttgtcgc gccgcaactg gctcagcacc atcgccgcct gttcgccgcc
      301 catcaccgag attcgggcgt tcggccacat ccagagaaaa cgcgggctgt aggcgcggcc
      361 gcacatgccg taattgccgg ccccgtagga gccgccgatc acgaccgtga atttcggcac
      421 cgccgcggtc gccaccgccg tcaccagctt ggcgccgtcg cgcgcgatgc cgccggcttc
      481 gtatttcttg ccgaccatga agccggtgat gttctgcaga aacaccaggg ggatattgcg
      541 ctggcagcac agctcgatga aatgcgcccc cttcagcgcg ctctcgctga acaggatacc
      601 attgttggcg atgatgccga ccggatagcc ccagatatgg gcgaaaccgc agatcagggt
      661 ctggccatac agcttcttga attcgtcgaa ctccgaaccg tcgaccacgc gcgcgatgat
      721 gtcgcgcacg tcatacggtt ttcggccgtc ggctgggacc acgccataga tttcctctcg
      781 cgcaaacaag ggctcgcgcg gctcgcgcat gttcaggttg gcgcgggctg gctgcttcaa
      841 ggtggcgacg atgcgccggg caattccgat cgcatggctg tcgttctgcg cgtagtgatc
      901 ggtgacgccg gactgccgcg agtgcacatc ggcgccgccc agctcttccg cggtgacgac
      961 ctcgcccgtg gccgccttca ccagcggcgg tccgccgaga aagatggtgc cttgattgcg
     1021 cacgatgatg ctctcgtccg acatcgccgg gacataggcg ccgccggcgg tgcaggagcc
     1081 catcacgatc gcaatttgcg gaatgccggc cgccgacatc tgcgcctgat tgtagaagat
     1141 gcggccgaaa tgtcgttcgt cggggaaaat ctcgtcctgc tgcggcagaa acgcaccgcc
     1201 ggaatcgacc atgtacacgc aaggcagatt gttctgccgc gcgatatcct gcgcgcgcag
     1261 atgcttcttc accgtcatcg gataataggt gccgcccttg atggtcgcat cgttggcgac
     1321 gatcacgcat tcgcggcccg atatccgccc cacgccggtg atgacgctgg cggaatgcac
     1381 gtcgccgcca tagaggccgt aggccgccag cggcgataat tcgaggaatg ccgtgccggg
     1441 atcgaccagg agatcgacgc gctggcgcgc cagcatcttg ccgcgcgagg tatgcctggc
     1501 ccgcgaggtc tcgccgccgc ctccggcgac ctgatcgagc ttttcgcgga gttccgcgac
     1561 cagcagccgc atggcctcgg cattgcctgc gaagtcagac gaggtggggt cggtactgga
     1621 atgaagcggc atggccttca gcggtttatg gtgcgccgca atctgggcat tggggctcgc
     1681 gtcatggtgt cattgcgccg ccgccttagg caacccagaa ctttccggcc cacggcactg
     1741 cccggggatc cgccctggac gattggaccc tcctaatggg tccagggttc ggcgcgccgg
     1801 aatgcaaaat tgtcggcata ggcggcctgc cggcgcgccg gctccttcgg ctcgctcacc
     1861 tgatagggga tgccctcacg ctcgcaatag gccacggcct cttccttgct gtcgaagcga
     1921 agggtgagtt gctgcttcat gtcgccggaa ctggtccagc ccatcaaagg ctcgatcgcc
     1981 tgcggctgct cgggctcata atcgagctgc cattcgcggg tcttggcggt ccccgattgc
     2041 atcgcgtttt tggcgggctt aaagatgcgt gcggtcatgg atgtcaggat cccctgaata
     2101 tgcgacatga agcggttggt ggaagcggcc gggatagtat agaaacggct ttcaggaatt
     2161 tgaccggtga ttccgaggct tggatgttac gttgtcccga ccggtatagt cattatgctg
     2221 cgctgtgaca atcttggccg gtcgcgcggc cgacgcgccc cgcccgctgc ccgatcttct
     2281 cgaaagcatc acttcgaaac ggaccgatga aaatcaacgc aaccctccct gaaaaaggac
     2341 gcgatctgcg gctcgatttg tttcgcggag tcgcaaactg ggcgattttt ctcgatcaca
     2401 ttcccgacaa cgtcgtgaac tggatcacca cgcgaaacta cggcttcagc gatgccgcgg
     2461 atctttttgt ttttatctca ggctataccg cgtcctttgt ctacgcccgg atgatgatcg
     2521 aacgcggctt tatcgtcggc gccacgcgtc tgaccaagcg cgtctggcag ctctacgtcg
     2581 cgcacgttat cctgttcgtc atctatatcg tttcgatagg ctatgtcgca cagcgttaca
     2641 gcgatccgga catcatcaac gaattcaatg tggcaggtct cgtcgacaat ccgatcgaaa
     2701 ccctgcgtca gggattgctg ctgaaattca agcccgtcaa cctcgacgtg cttccgcttt
     2761 acatcgcgct gatgggcctt tttccgccgg tgctgtggat gatgctgcgc cagcccaacc
     2821 tgacgatgct ggcctcgatt gtgctgtggc tcgccgcccg gcagttcggc tggaacctgc
     2881 cggcctatcc cgccggcgcc tggtacttca acccgtactg ctggcaggtg ctgtttgtct
     2941 tcggctcctg gtgcgcgctc ggcggtgccc gaaagtcgat gtcgttcatc aacgcgccca
     3001 tgacgctcta cttttgcctt gcgtatcttg tcctggcgct ggtgatgacg atggccggga
     3061 aatttccgga tttcggcgcg ctgttcccgc actggctcta tgaaaccttc aatcccaacg
     3121 acaagaccaa tctcgccccc tatcgcttcg tgcattttgt ggtgatcgtg atcctggtca
     3181 tccgattcgt gcccaagaac tggccggggc tggagtggcg gattttcgat cctgttatcg
     3241 tctgcggtca gcagtcgctc gccgtgttct gcgtcggtgt gttcctgtcc tttgtcgggc
     3301 atttcgaact gatgatgagt tcgggctccc tgttcgcgca gattttcgtc agcgtgacgg
     3361 ggatagcgat catgaccatc gtcgcttact acatctcctg gtcgaaaagg caggacaagc
     3421 cgcttcccaa gccggcggcg gcgccggcca aggccgggtg ataaggccgg gtgacgcgtt
     3481 ttcttgacgc gaaccggttt ccaccccgga tcaagtccgg ggccggcttt cgcttgaaaa
     3541 cgctttaatt cccgaatctg aagtttgcgc tacgcgcggg gcttggtgac cagtctgcct
     3601 ttcgatgaaa acccgtccca ctcggtggac cagccgaatc catgctggct ccgctttccc
     3661 tgcagcgcga agatggcgcc ctgaggatcg atgcatcggg ccatccagtt gccgcccggc
     3721 acttcgattg ggccctcgaa gatctggcca ccacccgtct tcacgcgctc cgaggccgcg
     3781 tcgatgtcgt cgacattgaa gtagtagagc cagtatggga ccggctccac cggacgcttg
     3841 gtaaacatgc caccgatcgt ctggccgccg gcggcgaaca actgataggt gtttgtcggg
     3901 gcgatttcgg tgttcgcttt ctgccagtca aaaagttcgc cgtagaaagc gaacgccttc
     3961 tcccagttgg tggcgagcaa ctcatgccag cccacgtgcc ccggcctgtc cagttcggca
     4021 ggctgctgtc ggccgggttt tttcggccct tcgatcaacg caagcgttgc cgtttgcgga
     4081 tccgcgacga ctgaaatgcg gccgatattg gtgttggtcg gcgggacgta cacggctccg
     4141 cccagacgct tgatgcggtc ggaagtcacg ccggcatcgt cgacgccgat ataccccatc
     4201 cacctcggcg tcgcgcccat tttcctcgcc tcctccggca agtccatcag tccgcttacc
     4261 gaggctgctc cggttgtaaa caaagtatag gccagctcgg gcgtcgacgc gtcctgggtg
     4321 ccccagccca cgactgcagc atagaaggcc ttggcggccg ccatgtccgt tgtcatcagt
     4381 tcgtaccagg cgaaacgtcc atgatagtcg accatgtcgg ttgttccgtt cgagccacca
     4441 tacaaccgca gcgcagcatc gagccgaccc ggcttcgttt ccggcacatg gttgaggggt
     4501 caagcctgtc ggcctgaata atggcggggc ggcgcgaatg caaggacgca cgcaccgtca
     4561 gggcaataga ggatcgtgta gatgatgtga tgaatatgtg gcgcagactg aacatgtttt
     4621 atgaacacat cgttcatgaa cctgaactac gagaacttga tatgtcgctg gcgcttgagt
     4681 gccttgccgg tcttgcacgg cgcagaagca aagcctacaa tcgccgcgct ggactcgaaa
     4741 acatttctgc aatgctatgt atggaggatg caactatgcc acgggcgaag caagcttcga
     4801 agcgcaagcg tggaacgaaa gccgtcccgg cgttgggagc cgccggattg accttttcac
     4861 tgatgggggg tgcatccgca gcggccgtgc cgacacagga cgtgccgcgg gtgccgaacg
     4921 ttgcaccaag tcacgaaatc acgcttggtg atgaggaaat ctccgacgtc agcttggcga
     4981 cgttctatgt cttcgacaag gagaacacag gatcgcttcg cggcatggtg caggaagccc
     5041 gtggttgcgg atgcggtggc tgcagagggt gcagaggatg cagaggatgc cgcggctgcg
     5101 gaggttgtag atgcggcggt ggctggggtt gcggcggcgg ctgcggaggc tgctgcctgt
     5161 cgtggggcgc ttgccgcctg tgctaggcta ggccgcgttc cgaatccatt gacaaacgca
     5221 aggcgtcatg gccgggttcg acgaggtcga cccggccaat tcgtgtcttc ttggcggtcc
     5281 tccaaatgtg ggtagccaga cgcgcgcatg acggccgagc tgatcttcaa cccatcgaga
     5341 acggccggcg cgcgctggac cagggagctc cggtgcccgg agagtgcgcc ccacagcggc
     5401 gatccggagt ggcaagcaac atgcgtgtaa tgacctcatg acgggccata ccaagacccc
     5461 ttccaagcag cacaccggca aagaagttct gcagttcgcg ccgaatttca ccgcctatgt
     5521 gctgcccccg gatgtcgtgt gcctctattc cgaggaccgc aaattcttcc ttcacggcga
     5581 gctttattgc gccctcgcct ccgcgatcgg aaaaaacggc agaagcctgc gcgaactggt
     5641 tggccggctg gagcaggatt ttccatccga caaaatcaac gaggcgctca agcggttgct
     5701 cgagcgccgc tatgtcgtgt cggtaacgaa ctcttccgca agcaccgtcg ccggctactg
     5761 gtctagtctc ggtctgccgc ccgggatggc ggaaacaaat cttgagaatt gtcgcgtgcg
     5821 cgttcaatcg atcgatgtgc agggcgctgc cgaactcgat gcggcgctgc gtgaacttgg
     5881 cgttcgcgtc gtcaagagtt ctcccgacct gaccgtcacg ctcgtgaacg actatctcga
     5941 acggcaactg gccgagctga acctgcagcg tgtatcggat aagacgccgt ggctgctcgt
     6001 gcaaccctcc ggggcctttc ccctggtggg acccgtgttc atcccgggcg aaagcgcctg
     6061 ttggacctgt cttttcgatc gcatgatacg gaacagggag gtcaaggggt ttctcgatcg
     6121 cggaccggcg caccccgtcg ccgtttcacc tctcgtccgc aacgcggtcg ggcaaggcgg
     6181 catccagctt gccgcgctcg aaatcgcgaa agcgatcgcc accggttttc ggaccgagct
     6241 gcgcgatcac attgtcagcc tcgacctgcc gggcgcaacc atcgcaaagc attacgtggc
     6301 ggcccgcccg caatgtccga cctgcggccg caagaagctg cgggacccgc gccgggcacc
     6361 ggtgccgatc gagcctggcc ctggcgccaa gctggtgatg acaagcgggg gataccggac
     6421 cgtgtcgtcg cggaccacgg tggcgcgttt ccgcaagcac gtgagcccgc tcaccggtgt
     6481 ggtcacgcgg ctcgagcgga tcgaagccga tttgcccatg aacaccaact tctttgcgca
     6541 gcacaatttt tcggcgccgg cccggaacgt cgatgagctc aggtcgggac tgagcggcgg
     6601 cagcttcggc aagggctcta ctgccgagca gggtgaagcc agcgcgctga tggaggcgat
     6661 cgagcgttat tccggaattt atcaaagcga cgagatccga ctgacacggc ggttcgcgga
     6721 ttttgcgccg ggcgatgcca ttcttcccaa cgacgtcttg ctgttcagcg aggcgcaaag
     6781 ccagccagac gaaacgcaaa ccgacgattc gcaccatcaa atggtcccgg ctcgcttcga
     6841 tcaatcggcc aagatcgagt ggtcgccggt ctggtcgctg cgcgaccaac gcttccgata
     6901 tctgccgacc agcatattgt attttttcta cagcggtccg gccgccttcc acgcggattc
     6961 caacggctgc gccgccggca acacgctcga ggaggcgatc gtccagggat tcctcgaact
     7021 ggtggaacgg gattcatatg caatctggtg gtacaaccga gtgcaacgcc cggaagtgga
     7081 cctcagccaa ttcgacgatt cctacgttcg cgatctgcaa acccagctcg ccgaaaccgg
     7141 gcgacgggtt tgggtgctcg acatcaccag cgatcttggc gtcccgacat atgttgcgat
     7201 cttgcactgg atgcaaaacg gccaggaaaa tatcgagttc ggctctggcg cgcacttcga
     7261 caagcgcatc gccctgctgc gggcgttgac cgagctgaac cagttcctgt cgatcggcct
     7321 gatgggtggc gggaccggcg agaaatcgag cctcgacggc atcacgccac tgcgtctgga
     7381 ggaccatccg tttttgaagc ctggcggcaa tccgacggcc caatccgaag acgacttgaa
     7441 agtcccgctc gacaacacgc gcgggcaggt cgatgcctgc gtgaatatcg ccaagcgcgc
     7501 cggcctcgat tttctcgtgc tcgatcagac gcggcccgac gttgaggtcc ccgtcgtcag
     7561 ggtgatcgtg ccggggctgc ggcatttcta tctccgcttc gcaccgggcc ggctttacga
     7621 tgttccggta aagcttggat ggctcgatcg gccgttaccg gaaagcgaac tcacgccgtt
     7681 ccttccgcac acctgagggc catccggctt gcgcgctccc ggaaaaaagc gagggcgaaa
     7741 aatcgcacca tcagtcgttt ccgcctggct gagcggtcac atcacgcttg aagcgcaggc
     7801 cgacggaaat atcgtcgcct gcttcgatgg ctattcggtc ggcctcggac agttcagcgc
     7861 cggcgccgtg gcgcgcgcgc aggacctgcg cacaggtctg tcgctcgcct cctttgcgtc
     7921 cgacggccgc acggccgaca aggaaatcga tctgctggtt cagcgcctgg cccggcgcgg
     7981 cctgctggag taccgcctcg ggccatcggg tgacggtaac gccctggtcg tcatcgaacc
     8041 gcagattccc gattactggc cgcgaacgcc aaagctcggc gattcagaga tcatcgtgtt
     8101 gtcccggttc gcatatttgc gacggcgcgg ccatgaaatg gtgctggaat ccccgcgcgc
     8161 cggcgcgttg ttcaggattt gcgatccgaa cattgcgtcc gccctcgcgg ccctgtcgac
     8221 accgcaaaga atcagcaggc tccggcggca ggacaatttt ccaggaatcg ggctcctcgc
     8281 gctgctggtg gattgccaga tccttttcag gatcgacgcc gcgaacgacc aaggcctccg
     8341 gccaaccgag ggcgacgaca acctcgtgct ttgggacttt catgatctcc tgttccacac
     8401 ccacagcacg gagggccgac acgccaaccc gctgggcggg ctttatcccc atgccggcgc
     8461 gatagctccg cttccggcgg tgcggccgcg ctggcccggc aagaaaatcg atctgcagaa
     8521 gttttcagcc gagccttcgc ggctcaactc gccggtcgca aagctcctgc tggaacgtca
     8581 ttcgatgcgc gacttcgacg accagcagcc gatcacgctc gccgagcttg cacagcttct
     8641 cgatagcacc gcgcgtgtcc ggtcgagctg gaaaagcggg ctcgattttg gcgagggcgg
     8701 tcccgaaatc gaatatgccg cgaggccgta tcccgcaggc ggcagcgctt acgagctcga
     8761 gctctatctg accgtcgcga actgcgaggg actcgcacgc ggtttctatc actacgacgc
     8821 cggcagccac gcgttggtgc cgatcgccgt tggcgcgccg gagctcgaag ccacattggc
     8881 ggcagccgaa ttcgcaatgg acgcccctgc cccgccccag atcctgatca cgatcgctgc
     8941 gcgctttggc aggacttcgt ggaagtacac ctcgatcgcc tattcgctga tcctgaagga
     9001 tgttggcgta ttgatccaga cgctctacct gatggcaacc gatatgggcc tcggtggatg
     9061 cgcgatcggg accagccata tcgagctgtt cgcgaagatg acggggatcg aattccacgt
     9121 cgagggcccg gtcggtcaat tcgcaatcgg tcgcggcagg aacctggagg cttccgatta
     9181 gtggcaggac aagctcggtc acgcagaccg ggtgcccgcc ataaccatgg tcgcgacgtc
     9241 ggcataaatg ccgaccagag gcccgacgat cttatgggcc gcattcttcg agaccagcct
     9301 gtcgtgcagc acgagattgt cgatccccgt ggtcagaaag cagatgacgg catcgcgcgg
     9361 cgtctccacg atcggctcgc ccttgacgtt gaatgacgtg ttgatcaaca cgggaatgcc
     9421 ggtcaacgcc tcgaactccc tgagcagtcg atacaacatc ggattggttt gttcacgcac
     9481 ggtctgcacc cgcgccgtgc cgtcgacatg gacgattgcg ggaatcctgt cccgccattc
     9541 ggggcggacg ttcttggcga ccaacatgaa tggcgactcc tcctcgcctt cgaacacttc
     9601 cctggcccgt tccgccagca cgatcggcgc aaacggccga aacgcttgcc gatgcttgac
     9661 gcgcttgttc agaatatcct tcatttcacc tttgcgcgga tccgcaagca ggctgcgatt
     9721 gccgagcgcc cgcggtccga actcggagcc accctggaac cagccgatca cgcgctggtc
     9781 gacgagcaat tgtgccgtct cgcggcagat attctcgcag cgcaccgccg aggtctggat
     9841 gcgaaccaga aatttcccgg tggcgttgtc aatatcctga tcggtgtagc gcttgccgac
     9901 ataggcgtgg tccatgacga acgtacggcg ctgcgctaga atttcgagat agccgtaata
     9961 ggcgcagccg atcgcaatgc cgtcatcgcc ggctgccggc tgaatccaga cattctcgaa
    10021 tccggcctcg cgaacaatcc ggccgttggc cacgcagttc agcgcgacgc cgccggcgat
    10081 acatagattt ttggcgccgg ttgtttcgcg cagccaccgg gcgcgggcga gcagcacgtt
    10141 ttcggtgtca tcctgaaccc gccaggcaag atcctcccaa tgccgcatcg acgggcttac
    10201 ttcccaactg ccgccatcca tcacgtaggg ttgcctgaag tccgttgtcc agcgcggaac
    10261 atgcaacgtg ccgtcgctga gctcgagcag gtgtttgacc tgttcgcagc ggccataagg
    10321 cgccagcccc atcagttcgc cgcatttatt ccagtcgcca aaaatatagg tcgaggcccg
    10381 gctgtagagc gcgccgaggc caggcaggtt gtagaattca ccgctcagga agccacggtc
    10441 gggctccatc cagaccttct tcaggcattc gatccttgcg ccgctgaagg tgtaatagct
    10501 ttcggattcc cgggcgagtg gcgtggcggt atcggtggac ggatagggct cgacgacgtc
    10561 ggattgatag ctgccgacac cgtcgacaat catcacgaca ccatcttcaa agggcgacgc
    10621 cgcaaacgcg ctgtaagcgt gcgccagatg atgggatatc gacaccactc tgtcggaacc
    10681 cgacaggtag agtggatgtc tggaagcctc ggcccgttca tattcgggaa gaaagcccgg
    10741 catgtcctga tagaccagcc gctcttccat ttccgcgacg ggcaggatgt aacaatttcg
    10801 aacgatcaga tcgacatcat caagcgtgat gccttccgca tccaggcaat aatcgatcac
    10861 ttccttgtag aaacctgtgg cgtgcttttc acgcgtgatc cgttccttgg cgatggcgaa
    10921 tgcgatggcg ccgtcgcgca acaggcaggc gctcacatca tggtcatagg tattcaagcc
    10981 gagaacgtag gtgtgttgtt tggacatcgg cactcttgga ttgtggggat cttgatcgag
    11041 aagacgctcg ggcggcgaag tcatgtttgt cttttgaaaa tggcgtttgc caaatcgcgt
    11101 ctgccaaatc acgtctgtga cgtcacaatt ttgccgcgtc acatttcgcg aggtcacagt
    11161 tttggcagat ttgattcgtt cgttgatcga agaaatcgcc gctcaacgcc acaatatcag
    11221 cacgcgcgtc gatgtttgag ccgttcttcg atttccgccg atcaaaatcg gtaaattgag
    11281 ctcgctcgcc cttctcaatg gcgtcgcaaa aaagccctac gaacactatc aagaacacca
    11341 tattgtaaac gatgttaccc cgcgccggaa cgaaggcttg gtcttggaag ttgtgacggg
    11401 ccaggtctaa gttctggtga ttgaggcaga tatatctgcc catactgatc caggcttaat
    11461 cggcggactc gttacgcgtt agcagaaaaa tcaggtgcgt cgggcaaccg gcgcacctga
    11521 tttcgtgttg ggaaaagctg ttccctctct catggtgtat tcggtcgtcc cttcaatgca
    11581 cgcggcgaga gcggcactcg aaaaatcctg aagcctgcga tttcgggaaa tgcctccgca
    11641 gcttccggag acaccagagc caaaaccgca gccggcactt ctccactggc cacctgattg
    11701 atcgccgtcg tttgaccttc gctcagctgg acttcagccg cgcccgccgc cgcgatcgcg
    11761 gtccgaacac tgtggattgt tcttgtagcg ggacggcgat tgaaactacc gcggaatgcg
    11821 aatctccgat ggttgcaaaa cccaatgccg tgatggaggg actactcctg caccggatgg
    11881 agtaccggtg caccagacaa ggtatcttgg caccgccagc aacctgtgaa gaattttccg
    11941 tcgatggcac gctgctttga acctgctgcg gattccctag acaaacccta acgggcactg
    12001 aatgcccgta acttggccgc cggcggcgtg gtgctaccgg ctttgtattt accggtgatc
    12061 aatatgagta aagcgatcat caggctttcg atcccgatcg ggaccattgc catttcaagc
    12121 gtagccattt tcgcattcag ctgggtcggc ctggcgcttc tgggatatta ggcccaggct
    12181 tgcgtcgaga ggttttggtc gcaccgctgt cgcgtgcgcg cggaccagcg ctggctagca
    12241 gttgctgcag atcttcaatt gtcttgtcag cttcgtctcc gcggcccgat cggctgcttc
    12301 ttgatcctcg aatgcttcat cggcttgaga tttgggattt cggccggccg aagaaacgtc
    12361 gccgatcggt ccgacgataa tctctttctt cgggcgggta gtcttcttcg gccgattttc
    12421 agggacatcg gccgtctccg tcgggggcga gccgaggccg tcccaggcga tcggtgcggt
    12481 cggcgcgccc agcaccggcg cgtagcaacc gttccccgac acgcaacaac ccgcgagcgc
    12541 gacggcgccg acgagaaaga caatacattt ggatatcatc ggagtgtcca tttggcctgc
    12601 caggaacagc tatgacggtg atgagacgtc acttcaagct caacgcgttg cctcgttgcc
    12661 actaagctgc cactaaaaaa tgcagccgcg cagttccagc cgggcgtccg aggaggcccg
    12721 ggccgcgatc aatttgcccg caaggtcgct ctgctcagcc ccggcgcaac gcgattttcc
    12781 ggttctcaca aataggcttt cggcagcagc atatgaatgc ccttgttgcc atcgacgagc
    12841 tgcgtgacac gtagattgcc ggcgagcgag caaagcatat aggcttggtt gcgcgacagg
    12901 ttggtgcgct cgcaaatatg cttcaccatt tcgcgcaccg cctgcttggc ggcgtcgtcc
    12961 agatcctcgt cgaggccgat cgacatcaaa tgagtggcac tctccgcgaa cggccatgac
    13021 aggtccatgt ccttgcgaac cgtgaggcgg aaggttccgg tcacgccggt ctcgagtgcg
    13081 gtgatgcaca cctcgccatc gccttggacg ccatgtccgt cgcctgcaaa gaacagcgca
    13141 ccttcgttga atatcggcaa gtagagcgtc gtccccggac gcagttcctt gttgtccata
    13201 ttgccgccga agctgcgcgg caccggcgag ccgcaacgcc cccaggccgg cggaggcgcc
    13261 gtggcaatga tgccaaaaaa cggatcaagc ggaatttcca ggccccacgg catcacgcag
    13321 acattgcgct cgtgatcgac cttgggatga atggtctcat attcggtgaa ctcgtccggc
    13381 aacgtgccga gcagcggcag gatcgacaca aaaccccaat cctgtcgcac cttcaccttg
    13441 agaatatcga tctgaaggac gtcgccgggt tttgcgccct tcacgtagac cggcccggta
    13501 atgaaatgag gccccggtcc ctgaaccagc gtatcgaggg ccgcgagata gtcggccggc
    13561 accaggcttc gatcctccgg aagacattcc ttgccacctg ccgggaagga atgcagcgtg
    13621 accgtgtcgc cggatgcaat ctcgagcacg ggcggcgtgg cgctatcgat gtagccccaa
    13681 accatatttt cgggagacga ggaaatttcg tggcggcgcg acatgggctt gctccgttga
    13741 gtggttggga cctgtcagat ggcgatgtac cgggtcagga gttcacggct ggaagattcg
    13801 gtgcggcccg cctcggcgac gctgccgcgc gaaagcacca cgaaggcatc ggcgaggcta
    13861 atcgcgaagt cgagatattg ctccacgaga agcagcgaga tcttgccttt gaatgaggcc
    13921 agcacatcct tgatggtcgc gacgagattg ggctggatac cctcggtcgg ttcgtcgagg
    13981 atcaagaggc tgggacgcgt gaccagcgcg cgcgcgatcg cgagttgctg ttgctgcccg
    14041 ccggagagtg cgccacccgg acgccgccac atctgcttca acaccggaaa cagggcgatc
    14101 gtctcctcag ggatcgcggt gccgaaggtg ccgtgcgccc gtgcagccgc ctcgatattc
    14161 tctccgacag tcaactccga aaagatctcg cgaccctgcg gcacgtaagc gagtcccgcg
    14221 accgagcgtc gatgcgatga ccagcccgat atctcggtct cgtcgaccag gatgcggcct
    14281 cgcgtcaccg gaagcacccc catcaggcat ttcagcagcg tggtttttcc ggcaccattc
    14341 cggccgagaa cggccaggca ttcgccgggc atgatctcga agccgacctg acgcagtacc
    14401 tgtgcgctgc cataatgctg atccaggctc tcaacgcgga agcgcatggt tatcgtccca
    14461 gatagacgtc aatgacgcgc tcgtcggccc gcgcattggc catcgagcct tcgaacaagg
    14521 tcttgccctc gtgcaaaacg gtcacccgat cggcaatccg ttcgacgaag tccatgtcat
    14581 gttcgacgac aacgatggcg cgatcggcgc gcttcaggct gcgcaccagc gaaacggtac
    14641 gttcggtctc ttcatccgtc aggcccgcca ccggctcatc cagcatcagg attttggggt
    14701 ttgacgccag caccatgccg atctcaagcc actgcttttg cccatgcgcg agctcggacg
    14761 ccgaacggtt ggcaaggccc gccaatccca ccgcctcaag cacctcgccg atacgatcat
    14821 cgagcttctc tcttgaacgc gatcggcgca gaccgccctc cgccccgatc gcgatgtgct
    14881 gacgcacgtt cagcccttcg aaaatgcttg gcttctggaa ttttcgccgg acgccggccc
    14941 gggcaatcgc ggattcgctt gatcgcacga tgtcggtcag gtcgtcgaaa atcacccggc
    15001 cctgcttcgg ccgcgtgatg ccggagatca catcaagcag ggtggtcttg ccggccccgt
    15061 tcgggccgat cacggcgcgg acttcgccaa aatcaatcgc gagcgacaac atgtcgatcg
    15121 ccgtgaagct gccgaattcg acggtgacgg tatcaaccaa cagcgcgacg ccactcatca
    15181 gcgcagctcc ggcgtttcgg tgaggcgttt ggagatcatg ggccgcacga atttgaactt
    15241 ggcgagatcg acgagcccgt taggaaacac cagcgccacg agcacgatca gacccgacag
    15301 gataaagggc catagctcag gcgcggctgc ggaaagccag aacttgaccg cgttgaccag
    15361 aaccgcgccg atgaccgccc cgatcaggtg gccacgcccg ccgatcgcca cccagacggc
    15421 aatctcgagc gacaaatcgg gtgagagcag tcgaggattg atgatgccga cctgcggcac
    15481 gtagagcatt ccggccagag ccgcaatcaa tgcggacaga caccagatca cgagctttag
    15541 aagcagcgtg tcgtaaccaa gcgtgcgcag ccgcacttcg tcgtcccgtg ttgcgatcat
    15601 caatacgccg aagcgacttt ccagcagcag gcggcagccc agcagcgtca ggcccagcat
    15661 cagcaccgaa atcgccgcca tcgcgatgac gaccccgtgc agcccgatcg gccagccgaa
    15721 catgaccgtg aagccggtca tgccattgtt gccgccgaag ccggtatcgt tccggaacat
    15781 cagcaacatc gcgacataga ccagcgcctg cgtgatgatg gcgaaataga ccccgccgac
    15841 ccgggagcga aatgaaacat agccaaatac gccggcgatc agcagcgtca cgactgcggc
    15901 gacgacgatg gcataagagg agtagccgaa gccggcccaa tagaacggaa attccttcca
    15961 tcccatgaac tgcatgaagt ccggcacctt gccggtgacc tcgtaggaat gcttgagaag
    16021 atgcatcgcg accacgtagc cgccgatgcc gaagaacagg ccgtgtccga gcgatagaat
    16081 gccgagatat ccccatatca ggtcaagcga cagcgccagc accgcgaagg ttgcgagctg
    16141 accgatggcg ttcgccagat aggccgaaag gccgaacgga atcgcgatcg aaatgccaag
    16201 caaaagagca actgcgagca ccaccgccag caggatcaga aacggacgat ccgatccgag
    16261 cgacctcatt tgcggcgccc cttgatggcg aacagtccct ccggccgaaa ctgcagaaag
    16321 ccgatgatca tcaggaggac gatgaccttg gctgcaaccg cgccccacag cggctcgatc
    16381 agtacattga cctgtccgat gccaagtgct gcgacgatgg tgccggcgat cgtgccgacg
    16441 ccccccagca cgacgaccat gaagctatcg atgatgaagt tctggcccat ctgcgggttg
    16501 acgctgtaga tcggcgcgag cgccaatccc gccaggcccg caaggcccga gccgagcccg
    16561 aaggccagca tgtcgacgcg ccgcaccgga attccgatgc agccggccat ggtccgattt
    16621 tgcgtgaccg cccggatgtt gagacccagc ggcgtcattt gcatcaatgc ccaggtcagc
    16681 gccagcgtca cggtcgcgaa tgcgatcgcg aacatgcgat tccaggtaaa gatgaagtcg
    16741 ccgatgaccg gcaccccgcc ggtgacataa aacggcgtca caaactgcag gttctgggtt
    16801 ccgaagctga cacgcacgag attgatgaga aagagactga cggcccaggt ggccagcaaa
    16861 ctcatcaggg gccggcgata aagatggcgc agcaggaccg cttcgagccc gatcccgatc
    16921 gcagcggtga tgagaaagac gaagggaata gcgatgatca ggtaccagtc gagcaatccg
    16981 ggcgcgaggt ggcgcaggct ctcctgcacc agccaggtga cgtaagcgcc gagcatgatc
    17041 agttcgccct gcgccaggtt gatcaccccc atcagcccga agatgaccgc gaggccaatc
    17101 gccgccatga acagaatgct ggcgaaactc agaccgttgt agagcgtcgc cagaacgtcg
    17161 ccgaccgcga taccacggtc tatccttgcg gtggcggcgt cgactgcgcg cttgaagtcc
    17221 ggattggcgg cataggccgg gtcattcacg agcgggcgga ccagcgtcag cgcccgccga
    17281 tcgggggtcg ccgcgatgcg tccgagggct gcgatacgct tggccggatc ctgtgaattc
    17341 agcgccgcgg cctgcgccac cgccgatagc tgcgtcttca gaccgggatc ggcttcctcc
    17401 cgaaccgccg catcgaacaa ttgttcgggc aggttcgatg cctggcgctc cagcgtgcgc
    17461 agcgcggcgc tgcggcgtgc aggatccggt gcgctgcgca aggcgagcgc cgcttcggcc
    17521 gtctcgaaca gagccctgtt cttcaggctg agaagcggcg atcggctgcc ttcgccgata
    17581 ttgcgctgga ggccagttga agcgtcgagc gatcccacag cggatgccgt gacggcaccc
    17641 gtggcgtcgc aggtgagctt gcggtcaagc agggcgcggg cgatcaacgc cgcccagctt
    17701 cgatcgtcgg gcgagccatc cgccgcgatc cgggtcagcg aacgcccggc ctcccccatc
    17761 gccgtcgcgt cgccgcaaac cttgcctatc agatcgaccc gctgctccgc tccagccgcc
    17821 cacagatgag tggacgcaaa cgccgcgatg aagcaaaccg cagcaatgca atgccgacgc
    17881 atcatgccta ccgatcatgg ccggcgggat cagcccggca gccacttgag cctgcgatca
    17941 tgggtcagct gtaagggctg aacggaacag gcttgggtgc gtccttggat tgccacagaa
    18001 tgtcgaaacc ctgcttctcg ttgacggagc caatgaacac gccacgagac acatagttgt
    18061 tttcggccgt catctccaac ttgtagcccg a
//
LOCUS       DATNOI010000102         4241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2454481,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000102 DATNOI010000000
VERSION     DATNOI010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2454481"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VN968_03560"
     CDS             <1..459
                     /locus_tag="VN968_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL28354.1"
                     /translation="TMTVEDNLQLGAFRIAKAIAAKSFERVYSLFPRLKERRKQLAGT
                     LSGGEQQMLAMGRALMGAPKLLMLDEPSLGLAPIIVADIFRTIGELRSAGVSVLLVEQ
                     NAQAALKIADRAYVMELGEFILNGAAKDIATNQRVAASYLGFQHEGPSAV"
     gene            594..989
                     /locus_tag="VN968_03565"
     CDS             594..989
                     /locus_tag="VN968_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HXL28355.1"
                     /translation="MISTPIRRAAAKRLRANATPHERALWRALKELPIDGTHFRRQAP
                     IGPYVVDFFCPARRLIIELDGGHHNDDETAKRERERQLWLEREGYRVVRFWNSEIIGD
                     LTAVLERIYVELYGLRQAEAEPLNHRRRR"
     gene            complement(1087..2238)
                     /locus_tag="VN968_03570"
     CDS             complement(1087..2238)
                     /locus_tag="VN968_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL28356.1"
                     /translation="MKKFYLSAAAFTASLALPGLPAMAQTNEITIGITVTTTGPAAAL
                     GIPERNALDFVPKEISGVPLKVIVLDDGGDPTNATTNARRFVTESKADIIMGSSITPS
                     TIAVSNVANEAGIPHIGLAPFPITPDRAKWSVAMPQPIPIMGKVIYEHMKSHNVKTVG
                     YIGYSDSYGDLWFNDFKMQAVPMGMTMIDEERFARPDTSVAGQVLKLVAANPDAVLVG
                     ASGTAAALPQTALRERGYTGPIYQTHGAASMDFIRIAGKAAEGVLMASGPVMSPETQP
                     DSALTKKPGLALNTAYEAKYGPNSRSQFAGHSYDAFEVLKRVIPVALKTAKPGTGEFR
                     DAIRQALLTEREIACSQGVYNFTEKDRYGLDDRSRIILTVKNGKYTPAQ"
     gene            complement(2330..2749)
                     /locus_tag="VN968_03575"
     CDS             complement(2330..2749)
                     /locus_tag="VN968_03575"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXL28357.1"
                     /translation="MFVNRRDVQIQWGDCDPANIVYYPRYFEMFDDSTSIMFEAAGFS
                     KQDIVRKYGLVGIPMVDTRAKFHIASSHGDWITIESRVERFKRSSFEVTHKVFKGEAL
                     AIEAFETRVLVGRHPGDPARLKSAPFPEEIIERFTRG"
     gene            complement(2759..3520)
                     /locus_tag="VN968_03580"
     CDS             complement(2759..3520)
                     /locus_tag="VN968_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL28358.1"
                     /translation="MQLKDQAAIVTGGASGLGAATARRLAAQGARVAVCDLNAKLAES
                     VAAEIKGVAIVCDVSDAASAEAAIASASKTHGPARVLVNCAGIGVAKRVIGREGPMPL
                     ADFDKVIKVNLIGSFNMLRLATAEMSKLEPLATGERGVVISTASVAAYDGQIGQSAYS
                     ASKGGIVAMTLPIARELAQFGIRVLTIAPGLFLTPLLANLPQEAQDSLAASIPFPRRL
                     GQADEFASLALHMIDNPYLNGEVVRLDAALRMAPR"
     gene            complement(3552..>4241)
                     /locus_tag="VN968_03585"
     CDS             complement(3552..>4241)
                     /locus_tag="VN968_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269104.1"
                     /note="catalyzes the conversion of ferulic acid to
                     feruloyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HXL28359.1"
                     /translation="RPATSRSGHVGLPVSGNEAKLVPVNGKLEVRARGPNVTPGYWRQ
                     PELTTAAFDDEGFYKFGDAIKPADPKDFSAGFDFDGRIAEDFKLASGTWVSVGPLRAR
                     FIAACAPMVRDVVIAGINRDEVSALVILDLDGCRLINPTLPADDLVATASDSLIREAF
                     HERLTKFLATATGSSNRITRAILIDTPLSIDRGEVTDKGSINQRAVLDHRSRLIEELY
                     SPMPAAHVITL"
BASE COUNT          783 a         1312 c         1334 g          812 t
ORIGIN      
        1 accatgacgg tcgaggacaa tctgcagctc ggcgcgttcc ggatcgcaaa ggcgattgcc
       61 gcaaaatcgt tcgagcgagt ctattcgctg tttccgcggc tgaaagagcg gcgcaagcaa
      121 ctggcgggca cgctgtcggg cggcgagcag caaatgctgg ccatgggccg cgccctgatg
      181 ggcgcgccaa aactgttgat gctggacgag cccagccttg gcttggcgcc tatcatcgtc
      241 gccgatattt tccgcaccat cggcgagttg cgctcagccg gcgtgtccgt gctgctggtc
      301 gagcagaacg cgcaggccgc attaaaaatc gccgaccgcg cctatgtgat ggagctcggc
      361 gaattcattt tgaacggagc ggcgaaagac atcgccacca atcaacgcgt cgccgcaagc
      421 tacctcggct ttcagcacga aggcccgagc gcggtttagc gcgtccttgc tctaatttgg
      481 cggatgcgct ccacttaccc tcccctggag ggggagggtc gctcgtgctc ggcgatgcgt
      541 agcatcgtcc ggtgcgagcg gggtggggtg atctatccgc aagtgagatg catatgatct
      601 cgacaccgat tcggcgagca gcagccaaac gactccgagc taacgcgaca ccacatgagc
      661 gcgcgctgtg gcgcgctctg aaggagctcc cgatagatgg cacgcacttc cgacgccagg
      721 caccgatcgg tccctatgtc gtagatttct tctgcccggc ccggcgcttg atcattgaac
      781 tcgacggcgg acaccataat gatgacgaaa ccgccaagcg cgaacgcgaa aggcagcttt
      841 ggcttgagcg ggagggatat cgggttgtcc gcttctggaa ttcggagatc atcggcgatc
      901 tgacggcggt gctcgaacgt atttatgtgg agttgtatgg gttgcgccaa gctgaggccg
      961 agccactgaa ccaccgacga cgacgataga ccgtcacccc accccgcctg cttcggacga
     1021 tgctacgcat cgccgagagc aggcgaccct ccccctccag gggaggataa gcaaaagccg
     1081 gccccctcac tgcgctggcg tgtattttcc gttcttcacg gtgaggatga tgcgcgagcg
     1141 gtcgtcgagg ccgtagcggt ccttttcggt gaaattgtag acgccctggc tgcaggcgat
     1201 ttcacgttcg gtcagcaaag cctgacggat cgcgtcgcgg aattcgcccg tcccgggctt
     1261 tgccgtcttc aatgccaccg ggatcacacg cttgagcacc tcaaaggcat cgtaggaatg
     1321 tccggcgaac tggctgcggc tgttgggacc atacttcgcc tcataggcgg tgttgagcgc
     1381 aagacccggc tttttggtca gcgcgctgtc gggctgcgtc tccggcgaca tcaccggacc
     1441 cgacgccatc agcacaccct ccgccgcctt tcccgcgata cggatgaagt ccatgctggc
     1501 ggcgccgtgg gtctgataga tcgggcccgt gtagccgcgt tcgcgcagcg cggtctgcgg
     1561 cagcgccgcg gcggtgccgg aggcgccgac cagcactgca tcgggattgg cggcgaccag
     1621 cttcagcacc tgacccgcga ccgacgtatc cggccgcgca aagcgctctt catcgatcat
     1681 cgtcatgccc atcggaacgg cctgcatctt gaagtcgttg aaccagagat cgccgtagga
     1741 atcggaatag ccgatgtaac cgacggtctt tacgttgtgc gacttcatgt gctcgtagat
     1801 aaccttcccc atgatcggga tcggctgcgg catcgctacc gaccattttg cgcggtccgg
     1861 cgtaatcgga aacggcgcca ggccgatatg cgggattccc gcctcgttgg cgacattgga
     1921 cacggcgatg gtcgacgggg tgatggacga gcccatgatg atgtcggcct tggactccgt
     1981 cacgaaacgc cgcgcattgg tggtggcgtt ggtcggatcg ccgccatcgt cgagcacgat
     2041 gactttcagc ggcacgccgc tgatctcctt cggcacgaag tcgagcgcat tgcgctcggg
     2101 aatgccgagc gccgccgccg gaccggtggt ggtgacggtg atgccgatgg tgatttcatt
     2161 ggtttgcgcc atcgcgggca gtcccggcag cgccagggac gccgtgaacg ccgccgccga
     2221 cagataaaac ttcttcattg attgcttcct cccttggatg cttctttgtt tcgtaacgaa
     2281 aaaattcggc cgaccctaca gcgcttcttt agacgatgca cctcgcttgt cagccccgcg
     2341 tgaacctttc gataatctcc tccgggaacg gcgccgattt gagccgcgcc ggatcgcccg
     2401 gatgccgtcc gaccaggacg cgggtctcga acgcctcgat tgccagcgcc tcgcccttga
     2461 acaccttgtg ggtaacctca aaactcgaac gcttgaaccg ctcgacccgg ctctcgatgg
     2521 tgatccagtc gccgtggctc gaggcgatat ggaatttcgc ccgtgtatcc accatgggaa
     2581 tgccgaccag gccatatttc ctgacgatgt cctgtttgga aaagccggcg gcctcgaaca
     2641 tgatcgaggt cgaatcgtcg aacatctcga agtagcgcgg gtaatagacg atgttggcgg
     2701 gatcgcagtc gccccactgg atctgcacat ccctgcgatt gacgaacatg caatcttcct
     2761 aacgcggcgc catgcgcagc gcggcatcaa gccgcaccac ctcgccgttc aaataggggt
     2821 tgtcgatcat gtgcagggcg agcgaagcga actcgtcggc ctggccgagc cggcgcggaa
     2881 acgggatcga cgcggcgaga ctgtcctgcg cctcctgcgg caaattggcc agcagcggtg
     2941 tcaggaacag tccaggcgcg atggtcagca cgcgaatgcc gaactgcgcc agttcgcgcg
     3001 cgatcggcag ggtcatggcg acgatgccgc cttttgaggc cgaataggcg gattgtccga
     3061 tctggccgtc ataggctgcg accgaggcgg tcgagatcac gacgccgcgc tcgccggtcg
     3121 caagcggctc cagcttcgac atttccgcgg tcgcaagccg cagcatgttg aacgaaccga
     3181 tcaggttgac cttgatcacc ttgtcaaaat cggcgagcgg catcgggccc tcgcggccga
     3241 tcacccgctt ggcgacgccg atgccggcgc aattgaccag tacccgcgcc ggaccatggg
     3301 tcttggacgc actggcgatg gcggcttccg cggaggctgc gtcggagacg tcgcagacaa
     3361 tcgcaacgcc ctttatctcg gcggcaacgc tctccgccag cttggcgttg aggtcgcaca
     3421 cggcgaccct ggctccctgc gctgcgagcc gccgcgcggt cgcggcgccc aatcccgatg
     3481 cacccccggt gacgatggcg gcctgatctt tcaactgcat gatctctccc tgaagttact
     3541 gttcgagttt gctaaagcgt tatcacgtgc gctgccggca ttggcgaata aagctcttcg
     3601 atcaggcggc tgcggtggtc cagcaccgcg cgctgattga tcgaaccctt gtcggtgact
     3661 tcgccgcggt cgatcgatag tggcgtgtcg atgaggatcg cgcgcgtgat cctgttggaa
     3721 gaccccgtcg ccgtcgccag aaattttgtc agccgttcgt gaaacgcttc gcggatcagg
     3781 gaatcggacg cggtcgcgac gagatcgtcg gccggaagcg ttgggttgat cagacggcag
     3841 ccgtcgagat cgagaatgac caatgccgag acttcatcgc ggttgatgcc ggcaatcacc
     3901 acgtcccgca ccattggcgc gcaggccgcg ataaagcggg cgcggagcgg accgacgctg
     3961 acccaggtgc cgctggcaag tttgaaatcc tcggcaatgc ggccgtcgaa atcgaacccg
     4021 gcgctgaaat ccttggggtc tgccggcttg atcgcatcgc cgaacttgta gaaaccttca
     4081 tcgtcgaacg cggcggtcgt gagttcgggc tgacgccagt agcccggcgt gacgttaggc
     4141 cccctggcgc gaacctcgag cttgccatta acaggaacaa gcttggcctc gttgccggag
     4201 acgggaagcc cgacatggcc ggagcggctg gtggcgggcc t
//
LOCUS       DATNOI010000103        11674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_326563,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000103 DATNOI010000000
VERSION     DATNOI010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11674
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326563"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1507)
                     /locus_tag="VN968_03590"
     CDS             complement(221..1507)
                     /locus_tag="VN968_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HXL28360.1"
                     /translation="MNVSGTFWFDAPGDAARAAELRRVKALATLVLASTLAVFITAKA
                     LLHLHPAFGFVAAFAEAATIGGLADWYAVVALFRRPLGLPIPHTAIIQGNQHRIADKL
                     GEFIEVHFLETAPVEAKLRQIDFATFIADWLRDRKRSTDLARFTLRLLPEAFSATETS
                     GLMTFISRRITTQLQSIDLAPLAAGTLRAFVAEGRHQGLFDDILRGVHQALTQPETLA
                     MIRAKIRAELPTLLKLYRTDKFLVNKIVASATAFFEEVRGDPKHPFRGEFDRMLLSFV
                     DRLGSDPSYADRIDGLKRDLLARPELGDLARNIWSNVRSFIERSASGETQVLQQHLAR
                     MFVGAGEALAGDSELRGEINQGLVTVLRSFIADQKSGVSSFISDQVKAWDMGQLISLI
                     EINIGKDLQYIRFNGSLIGGLAGLGLYTIEVLLRLL"
     gene            complement(1754..2236)
                     /locus_tag="VN968_03595"
     CDS             complement(1754..2236)
                     /locus_tag="VN968_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HXL28361.1"
                     /translation="MTTDTNSVLNSVKEAFAPVTDALKNLQNLEVPEAARDFVKKTAG
                     TAKERAADLHAGTEKVTDAIETAVAGSVSEAAKISRNIQQAIYQDTEAFFAGIDKLAS
                     AKSLNEAVQIQSDMVRARGEVLMSRAKSTTEYLGKLVADGAKTAQDNLSKVATYGKNA
                     "
     gene            complement(2417..2794)
                     /locus_tag="VN968_03600"
     CDS             complement(2417..2794)
                     /locus_tag="VN968_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HXL28362.1"
                     /translation="MTQKLTVWYNTKCPVCNAGIDWQHNRLVRAARSGAIDFRDINLE
                     PDALSGFGLGVNDIRRRLHAVDADNRLYAGIDCAIAIWLRTPGDIWLGRLVGLPVIRP
                     VAGFAYDRFADLLYAWNRWKGRW"
     gene            complement(2906..3499)
                     /locus_tag="VN968_03605"
     CDS             complement(2906..3499)
                     /locus_tag="VN968_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL28363.1"
                     /translation="MSDTRSYPLHAKTDAGDIEFRLMSRADEAAVLDFAQKLPTHDLL
                     FLPRNISQPKVLSAWINEIERGDISSLLAVKEGKVVGCGTLVRDPHSWSPHVGEIRMV
                     VSQDVRGQGVGRALSQQTFALALGAGLEKLSVQMTVDQQAAIALFESLGFKAEALLRD
                     HVRDVEGKKHDIVVLGHNVAQVRAQMEAYGLPDAVQH"
     gene            complement(3496..4578)
                     /locus_tag="VN968_03610"
     CDS             complement(3496..4578)
                     /locus_tag="VN968_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL28364.1"
                     /translation="MNAAAGLDLQSIPDRIQSEVQRAIARSIKGVEYIASSGPSLGST
                     PKDILSVRGTMNLYHYRPIADEIYRVPILIVMATTNRGYILDLVPGQSFIEFLLKRGY
                     DVYMLDWTAPKPEEKSLRMEDYVLDFIPDCVRRVQQDSGETDVTVIGYCFGGVLSLLY
                     GSIFADGPMKNLICFTTPVDFREMKLFQNFSDKRHFDVDRLIDSVGNVPPELILSSFE
                     MLRPASRAASQIQLWENIWNDEYVKSYRMFDRWATDTLPLAGEYFRAITKDLMWDNKL
                     YNDTMSVGGRPADIAKITVPILHAVAEHDHIVPYDAAKPLIQKIGSADKQEVMLKGGH
                     VSLVAGANAIKRLWPKLDSWLGERST"
     gene            complement(4575..4961)
                     /locus_tag="VN968_03615"
     CDS             complement(4575..4961)
                     /locus_tag="VN968_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28365.1"
                     /translation="MADKSNDPVAMWQNMIGEMEKGFNSFANQAMASPEFSKVVNQVG
                     GASAGAQKQLSDLMEKYLVGMNLPSRAQMVSMAERLQSIEGQLDEIKAMLHQMHNNPG
                     APAGGLAAAPRPPRTKRPPPPGGEQK"
     gene            5140..6060
                     /locus_tag="VN968_03620"
     CDS             5140..6060
                     /locus_tag="VN968_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL28366.1"
                     /translation="MNVLAHQPPQIARANGIELCYEIFGEAGAEPMLLIMGLGAQMIH
                     WDDDFCWQLAARGFRVIRFDNRDIGKSSKMTGGKRLTAVELLKLRFLKIPVAAPYKLS
                     DMALDVTGLMDVLGIKSAHLVGASMGGMIAQELAISFPQRVRSLTSIMSTTGNPKVPP
                     PTREATAILMAPPPASKEEYYARFAQTWKILRVGSFPLDESLDRARAERTYQRGLSPA
                     GVGRQFRAILASGNRKRRLASVKAPTLVIHGTVDPLVRPEGGKDTAASIPGAKLLMIE
                     GMGHALPIPMWPEIIDAIDKHAHGATAKAA"
     gene            complement(6200..9439)
                     /locus_tag="VN968_03625"
     CDS             complement(6200..9439)
                     /locus_tag="VN968_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXL28367.1"
                     /translation="MASTNRLSPIPHPPTKPVVGNMLSLDSTAPVQHLVRLTRELGPI
                     FWLDMMGAPLVIVSGHDLVEELSDEKRFDKAVRGSLRRVRAVGGDGLFTADTSEPNWS
                     KAHNILLQPFGNRAMQSYHPSMVDIADQLVKKWERLNGDEEIDVVHDMTALTLDTIGL
                     CGFDYRFNSFYRRDYHPFVESLVRSLETIMMTRGIPLEGIWLKKRRQELARDVAFMNK
                     MVDEIVAERRRNAEAAEGMKDMLGAMMTGVDRATGEQLDDVNIRYQINTFLIAGHETT
                     SGLLSCTLYALLKHPEVLKKAYEEVDRVLGPDTDARPTYQQVTQLTYITQILKEALRL
                     WPPAPAYGIAPLQDETIGGKYKLKKNTFITILVMALHRDPSVWGPNPDAFDPENFSRE
                     AEAARPVNAWKPFGNGQRACIGRGFAMHEAALAIGMILQRFKLIDIHRYQMHLKETLT
                     IKPDGFKIKVRPRSEKDRVAYAGRTTTKAAVSTGAPAPRARTRPGHNTPLLVLYGSNL
                     GTAEELAARVADLAEVNGFATKLAALDDFVGKLPQQGGVLIFCASYNGAPPDNATQFV
                     KWLGSDLARDAFSNVRYAVFGCGNSDWAATYQSVPRFIDEQLAAHGASNAYVRGEGDA
                     RDDLDGQFESWFAKLRPLAVKQFGIDTSFDRSADDEPLYRIEPVAPTAVNAIVALGGA
                     APMKVLVNTELQNKLGANASDRSTRHIEVQLPAGVTYRVGDHLSVVPRNDPVLVDSVA
                     RRFGFLPADQIRLQVAEGRRAQLPIGDTVSVGRLLSEFVELQQVATRKQIQIMSEHTR
                     CPVTKPKLLAYVGDDAAASEHYRADILGKRKSVFDLLEEHPACELPFHAYLEMLSLLA
                     PRYYSISSSPSGDPQRCSVTVAVVDAPASSGRGIYKGICSNYLAGRRAGETIQATVRE
                     TKAGFRLPNEAAVPIIMVGPGTGLAPFRGFLQERAARKAKRAALGPAMLFFGCRHPDQ
                     DFLYADELKAFEASGITELHTAFSRAEGPKTYVQNLVAAQKERVWSLIEDGAIIYVCG
                     DGGKMEPDVKAALVAIYRERKGADAEAGLRWIDDLGTSNRYVLDVWAGG"
     gene            complement(9649..10644)
                     /locus_tag="VN968_03630"
     CDS             complement(9649..10644)
                     /locus_tag="VN968_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL28368.1"
                     /translation="MIEMPPLQFAETNGIRMGYYEAGPKTDTPPVILCHGWPEIAFSW
                     RHQIKALGDAGIRVIAPDQRGYGATDRPEPIEAYDIEHLTGDLVGLLDHLGIDKAIFV
                     GHDWGGFIVWQMPLRHIDRVAGVVGINTPHTNRAPADPIELLRQRFGEHMYIVQFQDP
                     AREPDRIFGGRVEQTFDAFMRKPAPRSDTAPEEQVVAGIGASPRLNLAFPQMIAGYDA
                     KHDPRTPILSPEEKKVFVDTFTRTGFTGGINWYRNMSRNWLRSAQIDHNVRVPSLMIM
                     AENDSVLPPSAADGMEKIIPDLEKYLVRGSGHWTQQEKPEEVSAKLIEWRRKRFG"
     gene            complement(10691..>11674)
                     /locus_tag="VN968_03635"
     CDS             complement(10691..>11674)
                     /locus_tag="VN968_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HXL28369.1"
                     /translation="DSYQQLWTQSFDATAAAKGIDLPRSGKSDLGSILFDNAMFQIES
                     AVKFAGSLPAMLKGVSDVVGKISDPKSRDSIASMVSPSTILNKSISSERSFAGVSISL
                     SRAKVLAKQAGGKLNDVVLAISSGVVRRYLLERGALPAKAMTAAVPISLREEGNTDAN
                     NQVFGMICSIATNIEDPKARLEAIIAQSTKSKEMSHPLRALMPQVSNLSMLGAPILVQ
                     VLALLYSRSNLSDVLPPAANITVSNVPGPRQTLYAAGAELLHIFPVSISTHGLALNIT
                     VQSYRDQLDFGFIAGANIIPHVQVLCDMLPAEFAALEAAFAAPADLKSATG"
BASE COUNT         2170 a         3657 c         3602 g         2245 t
ORIGIN      
        1 tcggcacgaa gtcctgcaca ttcggcggca gcagcagcgt ctgatcgata ttccagggac
       61 gaaaatactt gctcatgggc ccaaggttga accagactcg ctcagagttg aacagccact
      121 attcggacag gctcctaggg aaccaccttt gacctttcaa gctgctcatg tggacgcggc
      181 cagggacgcc gcgtttagac gtcgcgccgt gattaaatag tcacagcagc cgcagcaaaa
      241 cttcgatcgt gtagaggccc aatcctgcaa ggccgccgat cagcgagccg ttgaagcgga
      301 tgtattgcag atccttgccg atgttgatct cgatcagcga aatcagctgc cccatgtccc
      361 aggccttgac ctggtcggag atgaagctcg agaccccgct tttctgatcg gcgataaagc
      421 ttcgcagcac ggtcaccagc ccctggttga tctcgccccg caactccgaa tcccctgcaa
      481 gcgcctcacc cgcgcccaca aacattcggg caagatgttg ctgcagcacc tgggtttcac
      541 cgctggcgct gcgttcgata aacgatcgca cgttcgacca gatgttgcgc gcgaggtcgc
      601 cgagttcggg acgcgccagg aggtcgcgct tcaagccgtc gatccgatcg gcataggacg
      661 ggtcgctgcc gagccggtcc acgaacgaga gcagcatgcg atcgaactcg ccgcggaacg
      721 gatgcttcgg gtcgccgcgg acctcttcga agaacgcggt cgcggacgcc acgatcttgt
      781 tcaccaggaa cttgtcggtg cggtagagct ttaacagtgt cggcaattca gcgcggattt
      841 ttgcgcggat catcgccagc gtctccggct gcgtcagcgc ctgatgtacc ccgcgcagga
      901 tgtcatcgaa caaaccctga tgccgcccct ccgccacgaa cgcacgcagc gtgccggcgg
      961 caagcggtgc gagatcgatc gactgcagct gggtggtgat ccggcggctg atgaacgtca
     1021 tcaaacccga cgtttccgtc gccgaaaacg cctcgggcaa gaggcgcagc gtgaagcggg
     1081 cgagatccgt gctgcgcttg cggtcacgca gccagtcggc gatgaaagtg gcaaaatcga
     1141 tctggcgcag cttggcctcg accggtgccg tttcgagaaa atgcacctcg atgaattcac
     1201 cgagcttgtc agcgatgcgg tgctgattgc cctggatgat cgcggtatgc gggatcggca
     1261 atcccagcgg ccggcggaat agcgccacca ccgcatacca gtcggcgagg cccccgatgg
     1321 tcgcggcttc cgcgaaggcc gcgacgaagc cgaaggccgg atgcaggtgc agcaacgcct
     1381 tggctgtgat gaacaccgcg agcgtgctcg ccagcaccag ggtggccagc gccttgacgc
     1441 gccgcaactc ggccgcgcgc gcggcgtcgc cgggcgcgtc gaaccagaat gtgccggaga
     1501 cgttcatggc ggctttcagg ggctcgaatt gattttcaat catagcgcga tgcagccacc
     1561 gccgtcatgg ccgggctcgt cccggccatc cacgtcttga gtatgtcgac aatcagaaga
     1621 aagacgcgga tgcccgcgac aagcgcgggc atgacgcgga gagatcgcga acaggcgcag
     1681 cggtcaaaaa gaaggcccgc gtgaagcggg cctctaaatg atctgccgat cgactgatcc
     1741 taaagcaagg cgatcaggcg ttcttgccgt aagtcgcgac cttggacagg ttgtcctgcg
     1801 cggtcttggc accgtcggcg acgagcttgc cgagatattc ggtggtggac ttggctcgcg
     1861 acatgagcac ttcgccgcgg gcacggacca tatcggactg gatctgaacc gcctcgttga
     1921 gcgacttggc ggaagccagc ttgtcgatgc ctgcaaagaa ggcttcggtg tcctgataaa
     1981 tagcctgctg aatgttgcgg ctgatcttgg cggcctcaga gaccgaaccg gcaacggcgg
     2041 tttcgatcgc gtcggtcacc ttctcggtac cggcgtgcag gtcggcagca cgctctttgg
     2101 cggtgccggc agtcttcttg acgaagtcac gggcagcctc gggaacttcc agattttgaa
     2161 ggttcttgag cgcgtcggtg acgggtgcga aggcttcctt gacggaattc agcacggaat
     2221 tggtatcggt agtcattgcg ttttctccat cctcaggttt tgagctatgg gctcaccccg
     2281 tccggattgg cccggggcga gatggttgtg tcatagttaa tggtgcatcg caacatcaat
     2341 gttgcggtgc gatatcacta atttgtgatc agccaatgat ttgagcggcc gagcccaata
     2401 atcaggctcc ctcgccctac caacggcctt tccagcgatt ccacgcgtac aacaaatcgg
     2461 caaaccggtc gtacgcgaag ccggcaacgg gccggatcac cggcaggccg acgagccgac
     2521 cgagccagat gtcgcccggc gttcgcagcc aaatcgcgat cgcgcagtcg attcccgcgt
     2581 agaggcgatt gtcggcatcg accgcatgca gccggcgcct gatgtcgttg accccaaggc
     2641 cgaagccaga gagcgcatcc ggctcgagat tgatgtcgcg aaaatcgatc gcgccggacc
     2701 gggcggcgcg aaccagtcga ttgtgctgcc agtcgatgcc ggcgttacac accggacatt
     2761 tggtgttgta ccaaactgtg agtttctggg tcatcgcacc ggtgcattcg agacgagtgg
     2821 agcatgatcc ggatatgtga ccttcttgcg ccggacggcc tcggcacggt cgttgcgcaa
     2881 cggttttgca accggtcgac tggacctaat gctgtacggc gtccggcagc ccgtaggcct
     2941 ccatctgggc gcggacctgc gccacattat gaccgagcac gacgatgtca tgcttcttgc
     3001 cctcgacatc ccgcacatgg tcgcgcagca gcgcttcggc cttgaagccg aggctttcga
     3061 acagcgcgat cgccgcctgc tgatcgaccg tcatctgcac cgaaagcttt tcaaggccgg
     3121 cccccaacgc cagcgcgaag gtttgctgcg acagcgcccg tccgacgccc tggccgcgaa
     3181 catcctgcga cacgaccatt cggatctcgc cgacatgcgg cgaccacgaa tgcggatctc
     3241 gcaccagcgt accgcaaccg accaccttgc cctccttcac cgccagcagg ctgctgatgt
     3301 cgccgcgctc gatctcattg atccaggccg acagcacctt gggctggctg atattgcgcg
     3361 gcaagaacaa gagatcgtgg gtgggaagct tttgcgcgaa atcgagcacc gcggcttcgt
     3421 cggcgcggga catcagacgg aattcaatat cgccggcgtc ggtcttggca tgcagagggt
     3481 aggaacgtgt gtcactcatg tcgatctctc accgagccag gaatccagtt tcggccacag
     3541 ccgcttgatg gcgttggcgc cggcgacaag gctgacgtga ccgcccttca gcatcacctc
     3601 ctgcttgtcg gccgagccga tcttttgaat caagggtttt gccgcatcat agggaacgat
     3661 gtgatcgtgc tcggcgaccg cgtgcaggat cggcaccgtg atcttggcaa tatccgccgg
     3721 ccggccgccg accgacatcg tatcgttata gagcttgttg tcccacatca ggtccttggt
     3781 gatggcgcgg aaatactccc cggccagcgg cagcgtgtcg gtggcccagc ggtcgaacat
     3841 gcggtacgac ttgacgtatt cgtcgttcca gatgttttcc cacaactgga tctggcttgc
     3901 cgcgcgggat gccggccgca acatctcgaa cgatgaaagg atcaactcgg gcgggacgtt
     3961 gccgacgctg tcgatcaatc gatcgacgtc gaaatggcgc ttgtccgaaa agttctggaa
     4021 cagcttcatt tcgcggaaat cgaccggggt ggtgaagcag atcaggttct tcatcggacc
     4081 gtcggcgaag atcgagccgt acaacagcga cagcacgccg ccgaagcaat agccgatcac
     4141 cgtgacatcg gtctcgccgg aatcctgctg cacgcggcgg acgcaatccg ggatgaagtc
     4201 gagaacgtaa tcctccatcc gcaagctttt ctcttccggc ttcggcgcgg tccagtccag
     4261 catgtagacg tcgtagccgc gcttcaacag aaactcgatg aaactctggc cgggcaccag
     4321 atcgaggatg tagccgcgat tggtggtcgc catcacgatc aggatcggca cgcggtagat
     4381 ctcgtccgcg atcggccggt agtgatacag attcatcgtg ccgcggacgc tcaggatgtc
     4441 cttcggcgtc gatcccagcg atgggcccga cgaagcgata tattcgacac ctttgatgct
     4501 gcgcgcaatc gcgcgctgca cctcggattg aatccgatcc gggatggatt gaagatccag
     4561 gcccgcggcg gcgttcattt ttgttctccg cccggaggcg gcggacgttt ggtgcgcggc
     4621 ggcctgggcg ctgccgcaag cccgcccgcc ggtgcgcccg gattgttgtg catctgatgc
     4681 agcatcgcct tgatctcgtc cagctgccct tcgatcgact gcagccgttc ggccatgctg
     4741 accatctggg cccggctcgg caaattcatc ccgacgagat atttctccat gagatcgctg
     4801 agctgctttt gcgcgcccgc cgaggccccg ccgacctgat tgacgacctt gctgaattca
     4861 ggcgacgcca tcgcctgatt ggcgaaggag ttgaacccct tctccatctc gccgatcatg
     4921 ttttgccaca tcgcgaccgg atcgttgctc ttgtctgcca ttggcgcccc ggaatgacga
     4981 cgagtccctc gcctgttttc aaatcatacc acattgtgcg ccacggcggt caacgtcgcg
     5041 aggtcgattt gtcagcctca ctgccgttcg caagccttct gcagtacacg aaaccgtgcc
     5101 ataaaatccc ggtaaaaccg atttgaggga aaatccaagg tgaacgtact cgcccatcag
     5161 ccgccacaga tcgcccgcgc caatggcatc gagctttgct acgagatctt cggcgaagcc
     5221 ggtgcggaac cgatgttgct gatcatgggg ctgggcgccc agatgatcca ttgggacgac
     5281 gatttctgct ggcagctcgc cgcgcgcggg tttcgcgtca tccggttcga caaccgcgat
     5341 atcggcaagt ccagcaagat gaccggcggc aagcggctga ccgcggtcga actgctcaag
     5401 ctgcggtttc tgaagattcc ggtcgccgcg ccctacaagc tgtccgacat ggcgctggat
     5461 gtgaccggcc tgatggatgt gctcggcatc aagtcggcgc atctggtggg ggcgtcgatg
     5521 ggcgggatga tcgcccagga actggcgatt tcgtttccgc agcgggtgcg ctcgctcact
     5581 tccatcatgt cgacgacggg caatccaaaa gtgccgccgc cgacgcgcga ggccacggcc
     5641 atcctgatgg caccgccgcc tgccagcaaa gaggaatatt acgcgcgctt cgcgcagacc
     5701 tggaagatcc tgcgtgtcgg aagcttcccg ctcgacgaat cgctcgatcg cgcccgcgcc
     5761 gaacgcacct accagcgcgg actgagtccc gccggcgtcg ggcggcaatt ccgcgccatc
     5821 ctcgcctcag gcaaccgcaa gcggcggctg gcatcggtca aggcgccgac gctggtgatc
     5881 catggcacgg tcgatcccct ggtgcggccc gaaggcggca aggacaccgc ggcctcgatc
     5941 cccggcgcaa aacttctgat gatcgaaggc atgggtcacg cactgccgat cccgatgtgg
     6001 ccggagatca tcgacgccat cgacaagcac gcccacggcg ccacggcaaa ggcggcgtaa
     6061 cccaccgtcg tcccggcgaa cgccgggacc catacgacgt gtcgtctcgt tttgataatg
     6121 ccgttggata agccgtttgt aatcgcgaat gccaggggtt atcggtcccg gcgttcgccg
     6181 ggaggacccg ctgaaatgat tatccccccg cccagacatc gagcacgtag cgattgctgg
     6241 tgccgagatc gtcgatccag cgcagccccg cctccgcatc ggcccctttg cgctcgcgat
     6301 agatcgcaac cagggcggcc ttgacgtccg gctccatttt gccgccatcg ccgcagacat
     6361 agatgatcgc gccgtcctcg atcaggctcc agacacgctc cttctgcgcc gcgaccagat
     6421 tctgcacata ggtttttggg ccctcggcgc gcgagaacgc ggtatgcagt tcggtgatgc
     6481 cgctggcttc gaacgctttc agttcatcgg catagagaaa atcctgatcg ggatgacggc
     6541 aaccgaaaaa cagcatcgcc gggccgagtg ccgcgcgctt ggctttccgg gcggcgcgct
     6601 cctgcaggaa gccccgaaat ggcgcgagcc cggttccggg gccgaccatg atgatcggca
     6661 cggcagcctc attgggcaac cggaaaccgg ccttggtctc gcgcaccgtc gcctggatcg
     6721 tctcgccggc acggcggccg gcgaggtaat tcgagcagat gcccttgtag atgccgcgtc
     6781 ccgaactcgc gggcgcgtcg accacggcga cggtgacgct gcagcgctgg ggatcgcccg
     6841 acggcgacga cgagatcgaa taatagcgcg gcgccagcag cgacagcatt tccagatagg
     6901 catgaaacgg caattcgcag gccgggtgtt cctccaggag atcgaacacc gatttgcgct
     6961 tgccgagaat gtccgcgcga taatgctccg atgcggcggc atcgtcgccg acataggcca
     7021 gcagttttgg ttttgtcacc ggacaccgcg tgtgttccga catgatctgg atctgcttgc
     7081 gggtcgcgac ctgctgcaat tcgacaaatt cggagagcaa tcgccccacc gacacggtgt
     7141 cgccaatcgg caattgcgcg cggcggcctt cggcgacctg cagccggatc tgatccgcgg
     7201 gcagaaaccc gaagcgccgc gccaccgaat ccaccagcac gggatcgttg cgcggcacta
     7261 cgctgagatg atcgccgacc cggtaggtga cgccggcagg caattgcacc tcgatgtgcc
     7321 gggtcgatcg atccgaggca ttggcgccga gcttgttctg cagttcggtg ttgaccagca
     7381 ccttcatcgg cgcggcgccg ccaagggcca cgatcgcatt caccgccgtc ggcgccaccg
     7441 gctcgattct atagagcggc tcgtcgtcgg cgctgcgatc gaaactggta tcgatgccga
     7501 attgcttcac cgccagcgga cgcaatttcg cgaaccagga ttcgaactgg ccgtcgagat
     7561 cgtcgcgggc gtcgccctcg ccgcgcacat aggcgttgct ggcgccgtgc gccgccagtt
     7621 gttcgtcgat gaagcgcgga accgactgat aggtcgcggc ccagtcgctg ttgccgcagc
     7681 cgaacaccgc gtagcgcaca tttgagaatg catccctggc caaatcgctg ccgagccatt
     7741 tgacgaactg cgtggcgttg tcgggcgggg cgccattgta cgacgcgcag aagatcagga
     7801 cgccgccctg ctgcggcaat ttgccgacga aatcgtcgag ggctgcgagc ttggtcgcaa
     7861 agccattgac ctcggccaaa tctgccacgc gcgccgccag ttcctcggcg gtgccgagat
     7921 tcgatccgta cagcaccaac agcggcgtat tgtgccccgg gcgggtccgg gcgcgcggcg
     7981 ccggcgcgcc cgtcgataca gccgcctttg tcgtggtgcg gccggcatag gcgacgcgat
     8041 ccttttcgct gcgcgggcgc accttgatct tgaagccgtc cggcttgatc gtcagcgtct
     8101 ccttcaggtg catctggtag cgatggatgt cgatcagctt gaaccgctgc aggatcatgc
     8161 cgatcgccag cgccgcctca tgcatggcaa agccgcggcc gatgcaggcg cgctggccgt
     8221 tgccgaacgg cttccaggca ttgacgggac gcgccgcctc ggcctccctg ctgaaattct
     8281 ccggatcgaa cgcatcggga ttgggtcccc agacgctggg gtcgcgatgc agcgccatca
     8341 ccaatatcgt gatgaaggtg ttcttcttga gcttgtactt gccgccgatg gtttcgtcct
     8401 ggagcggcgc aatgccatag gccggcgcgg gcggccaaag ccgcaaggcc tccttgagaa
     8461 tctgcgtgat gtaggtgagc tgcgtcacct gctggtaggt cggcctcgca tcggtatccg
     8521 gcccgagcac ccggtcgacc tcctcatagg ctttcttgag cacctcaggg tgctttagca
     8581 gcgcatacag cgtgcaggac aacaggccgc tggtggtctc gtggcccgcg atcaggaaag
     8641 tgttgatctg gtaacggatg ttgacgtcgt cgagctgctc gccggttgcc cggtcgacgc
     8701 cggtcatcat ggcgccgagc atgtccttca tgccttcggc agcttccgcg ttcctgcggc
     8761 gttcggcgac gatctcgtcg accatcttgt tcatgaaggc gacgtcgcgc gccaattcct
     8821 gccggcgctt tttcagccag atgccctcga gcggaatccc gcgggtcatc atgatggttt
     8881 cgagcgagcg caccagcgat tccacgaacg gatgataatc gcgccggtag aatgaattga
     8941 atcgatagtc gaaaccgcac agcccgatgg tgtcgagcgt cagcgccgtc atgtcgtgaa
     9001 cgacgtcgat ctcctcgtcg ccgttcagcc gctcccattt cttcaccagt tgatcggcga
     9061 tatcgaccat gctcgggtga taggactgca tggcgcggtt gccgaacggc tgcagcagga
     9121 tgttgtgggc cttgctccag ttcggctctg acgtatcggc ggtgaacagt ccgtcgccac
     9181 ccaccgcccg gacgcggcgc agcgagccac gcaccgcctt gtcgaaacgc ttttcgtcgc
     9241 tgagttcctc gacgagatcg tggccggaaa cgatgaccag cggcgcgccc atcatatcca
     9301 gccagaagat cggtccgagc tccctggtca gccgcaccag atgctgcacc ggcgcggtcg
     9361 aatccagcga cagcatgttg ccgaccacgg gtttcgtcgg cggatgcggg atcgggctca
     9421 gtcggttggt ggacgccatt gcgtgattgt ctcctgcgtc tctctagcta cgtgtcgtca
     9481 ttgcggggag cacttgcgac gcagcaatcc aggattcgtc attccgggat ggtccgaagg
     9541 accagacccg gaatctcgag attccgggtt cgacgcttcg catcgccccg gaatgacaga
     9601 tcgagtcgct tcgctcctgg caatgacggc gcggcaattt cgtgcgcctt aaccgaaccg
     9661 ctttctacgc cattcgatca gcttggcgct gacctcttcc ggcttttcct gctgcgtcca
     9721 atggccgctg ccccgaacca ggtacttttc cagatcgggg atgattttct ccatgccgtc
     9781 ggccgccgac ggcggcagca cggaatcgtt ctcggccatg atcatcagcg acggcaccct
     9841 cacgttgtga tcgatctgcg ccgaacgcag ccagttgcgc gacatgttgc ggtaccagtt
     9901 gatgccgccg gtgaagccgg ttctggtgaa ggtatcgaca aacaccttct tttcctccgg
     9961 cgacaggatc ggcgtgcgcg ggtcatgttt ggcgtcgtag ccggcaatca tttgcggaaa
    10021 agccagattc aaccgcggcg aagcgccgat gccggcgacg acctgctcct ccggcgcggt
    10081 gtcgctgcgc ggcgccggct tcctcataaa ggcgtcaaag gtctgttcga cccggccgcc
    10141 aaaaatcctg tcgggctcgc gcgcaggatc ctgaaactgc acgatataca tgtgctcgcc
    10201 gaaacgctgg cgtaacagtt cgatcggatc cgccggcgcg cggttggtgt gcggcgtatt
    10261 gatcccgacc acgccggcga cgcggtcgat gtgccgcagc ggcatttgcc agacgataaa
    10321 gccgccccag tcgtgcccga caaaaatcgc cttgtcgatt ccgaggtggt cgagcagccc
    10381 gacgagatcg ccggtcaggt gctcgatatc gtaggcctcg atgggttcgg gccggtcggt
    10441 cgcgccatag ccgcgctggt ccggcgcgat cacccgaatg ccggcatcgc ccagcgcctt
    10501 gatctggtgg cgccacgaaa acgcgatctc cggccagccg tggcagagaa tgaccggcgg
    10561 cgtatcggtt ttcgggcccg cttcgtagta gcccatgcga atgccgttgg tctcggcgaa
    10621 ctgcagcggc ggcatctcta tcatcgtcga tgtctcactt ttctggagca tgatccaatc
    10681 agatcatgcg ctagcctgtc gcgctcttga gatcggcagg cgccgcgaag gcggcttcga
    10741 gggcggcgaa ttccgccggc agcatgtcgc acaacacctg cacatgggga atgatgttgg
    10801 cgccggcgat aaagccgaaa tccagctgat cgcgatagct ctgcacggtg atgttgagcg
    10861 caaggccgtg ggtcgagatc gacaccggaa agatgtgcag caattccgcc cccgccgcat
    10921 agagcgtctg gcgcggcccg ggaacgttgg agacggtgat gttcgcggcc ggcggcaata
    10981 cgtccgacag gttggagcgg ctatacagca acgccagaac ctgaaccagg atcggcgcgc
    11041 cgagcatcga gaggttggag acctgcggca tcagggcccg cagcgggtga gacatttcct
    11101 tcgacttggt ggattgcgcg atgatcgctt ccagccgcgc cttgggatcc tcgatgttgg
    11161 tggcgatcga acagatcatg ccgaacacct gattgttggc gtcggtgttg ccctcttcgc
    11221 gcaacgaaat cggcaccgcg gccgtcatcg ccttcgccgg cagagcgccg cgctccagca
    11281 gatagcgccg gaccacgccg ctcgaaatcg ccagcacgac gtcgttcagc ttgccgccgg
    11341 cctgtttggc cagcactttc gcccggctca gcgagatcga taccccggca aagctgcgct
    11401 ccgacgaaat cgatttgttc aggatcgtcg acggcgacac catgctggca atgctgtcgc
    11461 gcgatttcgg atcggatatc ttgccgacca catccgagac gcctttgagc atggcgggaa
    11521 ggctgccggc aaacttcacc gcggattcga tctggaacat cgcgttgtcg aacaggattg
    11581 atccgagatc gctttttccc gagcgcggca gatcgatgcc cttggcggcc gccgttgcat
    11641 cgaacgactg ggtccaaagc tgctgatagg aatc
//
LOCUS       DATNOI010000104         6374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_824260,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000104 DATNOI010000000
VERSION     DATNOI010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824260"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..784
                     /locus_tag="VN968_03640"
     CDS             89..784
                     /locus_tag="VN968_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HXL28370.1"
                     /translation="MAQTDSAELPESDTLSERAAGLVERDIIAGLHAPGARLGISDLV
                     QRYEIGATPLREGLSRLISRGLIVGIGQRGFRVADVSREDLLDITRMRTVVEIEALRL
                     AIAQGDDAWEAGILSALHRMRRHIQRTGNDFREGAPDFDRLHKAFHTSLLAACGSKRL
                     LTAHSDLYDQAYRYRRVMMRAFDSGRKFVAAHDSLAERVLARDVDGARTMLASHLHST
                     LDIVYPSGKKDRS"
     gene            787..1590
                     /locus_tag="VN968_03645"
     CDS             787..1590
                     /locus_tag="VN968_03645"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28371.1"
                     /translation="MPVGARQAARCAGLERPQIAFSKVTLVLGGKTIVEDLSLDVKPG
                     EFLCVIGASGCGKTTALRLAAGLYQPTGGAVSFDGKPMLMPRRDIAIVFQDYGKALLP
                     WRTAAGNVSLALEASGVASSRRATRIDELLKKVGLPNHAGKYPAEMSGGMQQRLQIAR
                     CLAQEPKALLMDEPFGALDAMTRQGLQDEMLSLVRASEATVVFVTHDLEEAIYLGDRV
                     IGLSPHPGRIGIELEIDLPRPRDQLTTREDPQFLALRRELFDFIKVSET"
     gene            1587..2351
                     /locus_tag="VN968_03650"
     CDS             1587..2351
                     /locus_tag="VN968_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL28372.1"
                     /translation="MIPERVKALLLPVTALAALEIWARAIHLQSDSLAPPSAVAAALV
                     GAFQDGSILIATRDTLVSAFAGLSIGGAIGLAFGIALGIFHVFDRLMEVTIEAIRPIP
                     SIALLPIALIALGFGYRMEIVIVAFACIWPILILSRAAVGGIEPRLMEVSRALRLSPA
                     DRARKIIIPAALPRIFLAFRLAAGIALIVAVTVEIAINPIGLGAAIMTAQQALRPDLM
                     LAYLLWIGIVGYLLNTGLVVAQNHLFGRAALVRDPT"
     gene            2348..3115
                     /locus_tag="VN968_03655"
     CDS             2348..3115
                     /locus_tag="VN968_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL28373.1"
                     /translation="MNRSVLYWRAASFAVAAGFVGLWQMIANQRLVSPVFLPGPDRAW
                     ASLVRGFTDGDLWGKLVGTLGHMAYGWFVASIAGIALGAMIGSSRTMRSYVAPSLEFL
                     RPLPVSAIIPVAIAMLGLTQSMALFVIAFGAIWPMLLATIHGFAAVEPRLYEVSRSLH
                     MSRLAVIFKIALPSASPDILSGMRLSLTVALILSVVCEILAGLDGLGHWVLLAARSFR
                     SADLFAGVILLGFIGYLTAFAMSVVEKRLLRWRSSGH"
     gene            3301..4035
                     /locus_tag="VN968_03660"
     CDS             3301..4035
                     /locus_tag="VN968_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXL28374.1"
                     /translation="MPRREFIATIAGITALPVAALAQAVPPIAPQNGAALYSQYCSQC
                     HDAANSRAPGRSVLQSMKLEEVLRALGTGSMASIAQERSEAERTAIASFITGKGAEAP
                     PGSTDISGSQCRRRSVRARPRKRENINAGAASLMWQPGRCSPALSAAVTAIPGVVFSG
                     GVSGYLRAYATGDARLLWEVDTARDYATMNGVSARGGAMDGPGPTIVDGMLYVNSGYE
                     QWGGLPGPVGLRSQESLKTVSWSAMA"
     gene            complement(4944..6131)
                     /locus_tag="VN968_03665"
     CDS             complement(4944..6131)
                     /locus_tag="VN968_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL28375.1"
                     /translation="MDFQLTEQQRRMVAAVRDLAQGEFKANAQKYMDGTFPWENMKKL
                     ADIGVLGMSVPEEYGGLGLPVFDTALVLEEIAKTCYVTAMATLGEAGVQTRVIATYAP
                     NGIRERILPKVVSGDCILAVCMTEPHAGTDVANYRTNAIIRGDRIILNGTKTLISRAR
                     EAGMFVIFTRIDGKPGREGIGCVLLEPDTPGFEVTGTYHTMGGENLHEIQFNNCELPL
                     ENLVIRDDGFRKLLNAFNTQRCLNPSISLGLAEGAFEEARKYVRDRTVFGKAIGDYQG
                     VRWKLAEMYREIETARSILYRACVTANPFPDPFMAAVAKITCNEMSNRVTSEAIQLHG
                     GFGFTDEFAVSRLYRAARYGTLGGGTSETLRDLIGKRIMDAAETGAELLAYDMMDGAD
                     LAV"
BASE COUNT         1170 a         1930 c         1972 g         1302 t
ORIGIN      
        1 tcgacatcat gtcgtcgcaa aagatgctgc aaaccaaggt cgatctcaat aagctcatgc
       61 tgaactagcc gcagacggag tgtcgacatt ggcgcaaacc gattcagctg aactgccgga
      121 aagcgatacg ctgagcgagc gcgcggcagg cctcgtggag cgcgacatca tcgccggcct
      181 tcacgcgccc ggcgcgcgtc ttgggatcag cgatctggtc cagcgctacg aaatcggcgc
      241 gacgcccctg cgcgaaggcc tgtcccggct gatttccagg ggattgatcg tcgggatcgg
      301 ccagcgcggt tttcgggtcg ccgacgtcag ccgcgaggac ctgttggata tcacgcggat
      361 gcgcacagtg gtcgagatcg aggcgctgcg gctcgcgatc gcgcagggcg acgatgcctg
      421 ggaggcggga atcctcagcg cgctgcatcg gatgcgccgg cacatccagc gaaccggcaa
      481 cgacttccgc gagggcgcac cggatttcga ccgacttcac aaggccttcc atacctcgct
      541 actggcggca tgcggatcga agcggctgct tacggcacat tccgatctct acgatcaggc
      601 ctatcgctat cgccgcgtca tgatgcgggc cttcgacagc ggcaggaagt ttgtcgcggc
      661 acacgatagc ctggccgaac gggttcttgc gcgcgatgtc gacggcgcca ggaccatgct
      721 cgcgtcgcac ctgcattcga cgctcgacat cgtctatccg tccggaaaaa aggatagatc
      781 gtgagcatgc cggttggagc aaggcaggcg gcacgatgcg ccggtttgga acggccgcag
      841 atcgcttttt ccaaggtcac gctcgtgctc ggcggcaaga cgatcgtcga ggatctcagc
      901 cttgacgtaa aaccgggtga attcctctgc gtcatcggcg cctcgggctg cggcaagacc
      961 accgcactgc gtctcgcggc cggattatat caaccgaccg gcggcgccgt gagcttcgac
     1021 ggaaagccga tgctgatgcc gcggcgtgat atcgccatcg tgtttcagga ctacggcaag
     1081 gcgctgctgc catggcgaac cgccgccggc aatgtctccc tggcgctgga ggcgtcgggc
     1141 gtcgcctctt cccggcgggc tacccgcatt gacgagttgc tgaagaaggt cgggctgccc
     1201 aatcatgccg gaaaatatcc ggcggagatg tcgggcggca tgcagcagcg cctgcagatt
     1261 gcgcgctgcc tggcgcagga acccaaggcg ctgttaatgg atgagccgtt cggcgcgctg
     1321 gacgcgatga cgcgccaggg attgcaggac gagatgctgt cgctggtgcg cgccagcgag
     1381 gctaccgtgg tcttcgtcac ccacgatctg gaagaggcaa tctatctcgg cgaccgcgtc
     1441 atcggtctgt cgccgcatcc cggccgcatc ggcatcgaac tcgagatcga tctcccgcgg
     1501 ccgcgcgatc agctgacgac gcgggaggat ccccaattcc tggcgctgcg tcgcgaactg
     1561 ttcgatttta tcaaagtctc cgagacatga ttccggagcg cgtcaaggcg ttgctgcttc
     1621 cggtcacagc cctggcagcg ctcgagatct gggcgcgtgc gattcacctg caaagcgact
     1681 ctctggcgcc gccaagcgcc gttgccgcag cactcgtcgg agccttccag gatggctcga
     1741 tcctgattgc aacacgcgac accctggtct cggcgtttgc cgggctatcg atcggcggcg
     1801 ccattggcct tgctttcggt attgccttgg ggattttcca cgtcttcgac cggctgatgg
     1861 aagtgaccat tgaggcgatc cgtccaatcc cgtcgattgc actcctgccc atcgcgctga
     1921 tcgcgctggg tttcggatac cgcatggaga ttgtgattgt cgccttcgcc tgtatctggc
     1981 cgattctgat cctgagccgc gcagccgttg gggggatcga gccgcggctg atggaggtat
     2041 cgcgcgcgct gcgtctttct ccggcggacc gggcccgcaa aatcatcatt ccggcggcgc
     2101 tgccacgaat ctttctggcg ttccggctgg cggccgggat cgccctgatt gtcgcggtca
     2161 cggtggagat tgcgattaac ccaatcgggc tgggcgctgc gatcatgacc gcgcagcagg
     2221 cactaaggcc cgacctgatg ctggcctatc tgctatggat cggcatcgtc ggatacctgc
     2281 tgaataccgg tcttgtagtc gcacaaaatc atctgttcgg acgcgcggca ttggtgagag
     2341 acccgacatg aaccgctctg tgctgtattg gcgcgctgcc agctttgccg tcgcagccgg
     2401 cttcgtcggg ctgtggcaaa tgatcgccaa ccagcggctg gtgtcgcccg tcttcctgcc
     2461 ggggccggat cgggcgtggg catcattggt acgcggattt accgacggcg acctctgggg
     2521 caagctcgtg ggaacgctgg ggcacatggc ctatggctgg ttcgtcgcct ccatcgccgg
     2581 catcgcgctt ggcgcgatga tcggctcatc gcgcacaatg cgcagttatg tcgcgccctc
     2641 gctcgaattc ctgaggccgc tgccggtgtc cgcgattatt cccgttgcga ttgcgatgct
     2701 cgggctaacc cagagcatgg cgctgttcgt gatcgcgttc ggcgccatct ggccgatgct
     2761 gctggcaacg atccacggct ttgccgcggt cgagccgcga ctttacgagg tgtcgcgctc
     2821 gctgcatatg tcacggctgg cggttatctt caagatcgcg ctgccctcgg caagtcctga
     2881 tatcctctca ggcatgcgac tgagcctgac cgtggcgctc atcctatcgg tggtctgcga
     2941 aattctcgcc ggcctcgatg gcctgggaca ttgggtgctg ctggcggcgc ggtcgttccg
     3001 gtcggcagac ttgttcgccg gcgttatcct gcttggcttc atcggctatc taaccgcatt
     3061 tgcgatgtct gttgtcgaga agcggctctt gcggtggcga tcgagtggcc attgagtttc
     3121 ccgcctcctc gatcagccgg gatcgatccg gacgagcttg gattttgcga tgaacgtcca
     3181 cttcgcgcca gaagtggccg ttccggcaag gacgcgcaca gccatggcgg acattcgacc
     3241 gcttggatgt ctccgttgaa ggttctacgc tgcggtcata cccagccggg gcacggagcg
     3301 atgccgcgac gagaatttat tgcaacaatt gccggcatca cggctttgcc ggtcgcggcg
     3361 ctcgcgcagg ctgttccccc gatcgcgccg cagaacggtg ccgctctcta ttcgcaatac
     3421 tgttcgcagt gccacgacgc cgccaacagt cgcgcgccag gccgaagcgt gctccagtcg
     3481 atgaaactgg aggaggttct gagagcgctc ggcacaggga gcatggcctc catcgcacaa
     3541 gagcggtcgg aagccgagcg gacggccatt gcgtcattca taaccggaaa aggcgcggaa
     3601 gccccgcctg gctcgaccga catttccgga tcccaatgca ggcggaggtc tgttcgcgct
     3661 cgacctcgca agcgggaaaa tatcaatgca ggtgccgcca gcctcatgtg gcagccgggg
     3721 cggtgcagcc ctgcactctc cgcggcggtc accgccattc ccggtgttgt tttctcgggc
     3781 ggggtgagcg gctatctgcg cgcgtatgcc accggcgacg cacggctgct ctgggaggtg
     3841 gacacggcgc gtgactatgc cacgatgaat ggcgtgtcgg cccgtggggg tgccatggac
     3901 ggacccggtc caacgatcgt cgacggtatg ctctacgtga attccggcta cgagcaatgg
     3961 ggcggtctgc ccggtcctgt tggccttcga agtcaggaat ccctgaagac ggtgagttgg
     4021 tcggcgatgg cgtgatgcca ggccaatgtc gccaattgtg aagatttttg gatgccggcc
     4081 ccatgaagga ggtgccgcgc gcaccggggc cggcatccga aaaacttcac aaaagtggcc
     4141 gatcagcgat ttttgtatga tcgaggcgga acatcgccga gcagatcgag ctttagtcat
     4201 tggcctgcca ctgcccgttc caactgtcag atgattgcaa ctctacgcac caattttttc
     4261 ggcgtcacaa tcgcgccgta cgaaaatccc aattgaaaat gcaacctgca gagctcgacg
     4321 caaatcaggg gggagtgaat gcgacaagaa cgaacgctga ttaatcgcac ccggaaatcg
     4381 gatggctaca ccttcttttt tcagattgcg aacgatggtg gacggcgggc gtgttgtgtc
     4441 agggtagacg ccttaaacaa gcacgaggcg tcggctctct ttcggcagaa atggtccgtc
     4501 attgagttga tggcccgcga agacatcaac agcagggcca tcagcgacgg cgtcataaca
     4561 ctcgttatgc cttgatggac attctcgccg gtcaaacgac acctcatcac gcccgcttgg
     4621 cgccggaacc ggacgtcctc acaaggacgc ttttgaccac acatggcaga cgtctactga
     4681 gcaacggtgt tgctgctgcg gcatgagaag ctggcagatg tccggatgct cacgcgacag
     4741 gatgcatcca gcatttcggt ggtgtccggt tgttgcatgc cagcgacgcc cggcgatggg
     4801 acatgtccaa tcccaaaatt tagtcgttct gcgagcgagg gagcgcttcg gcgtgctcgc
     4861 atcgcgaccg tccgttcaat ggttggcgac gatgagacgc tcggaacgat cgtgcggcct
     4921 tccacgcgac accatttgga ttgtcatacc gcgagatcag cgccgtccat catgtcataa
     4981 gccaggagct cagcgcccgt ctccgctgca tccatgatcc gcttgccgat aagatcacgg
     5041 agcgtctcgg aggtgccgcc gccgagcgtg ccgtagcgcg ctgcgcgata gagacgcgat
     5101 accgcgaatt cgtcggtgaa accgaatccg ccgtgcaact ggatcgcctc gctggtgacc
     5161 cggttggaca tctcgttgca cgtgatcttg gccaccgccg ccatgaaagg atcgggaaac
     5221 ggattggcag tgacacaggc gcgatagagg atgctgcggg ccgtctcgat ctcgcggtac
     5281 atctcggcga gtttccagcg cacgccttga tagtcgccga tcgcctttcc gaacaccgtt
     5341 cggtccctga catatttgcg ggcttcttcg aaagcgccct cggccagccc aagcgatata
     5401 ctcggattga ggcagcgctg cgtgttgaag gcgttcagca gctttcgaaa gccgtcgtcg
     5461 cgaatcacga gattctccaa cggcaattcg caattgttga actggatctc gtggaggttc
     5521 tcgccgccca tcgtgtggta ggtcccggtc acctcgaaac ccggcgtgtc gggctccagc
     5581 agaacgcagc caataccctc gcggccaggc ttgccgtcga tccgtgtgaa gatgacgaac
     5641 atgccggcct ccctggctcg gctgatcagc gtctttgtcc cattgagaat gatgcggtca
     5701 cccctgataa tggcgttggt ccggtagttc gctacgtcgg tgccggcgtg tggctcggtc
     5761 atacagactg ccaatatgca gtcgccagag acgaccttcg gaagaattcg ctcgcgaata
     5821 ccattgggag cgtaggtcgc gatcacacgg gtctgaacac cggcctcacc gagggtggcc
     5881 atcgcggtaa cgtaacaagt cttggcaatc tcctcgagca ccagcgccgt atcgaatacc
     5941 ggcaagccaa ggccgccata ttcttcggga accgacatgc caagcacgcc aatatcggcg
     6001 agcttcttca tgttttccca aggaaacgta ccatccatat acttttgcgc gtttgccttg
     6061 aactccccct gagcgaggtc gcgcaccgcg gctaccatcc gccgctgctg ttcggtgagt
     6121 tggaagtcca ttgcttatcc ttgaagtgaa aaaggcgacg acccgatcgg cgaactggcc
     6181 gacccgcacc aacgagctga ccccgtttca ctgacggcta aatccgcaaa agcaatacta
     6241 ctggtgctcc tgccctgagg gatagtgcct gcgcatctgc attttccgcg ttatggagcc
     6301 ggtctctcgc ggatatgatg attataagtc gttttcgagc gcgcgtttgc cataacatcc
     6361 ttggcgcagg gaga
//
LOCUS       DATNOI010000105         7847 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2098274,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000105 DATNOI010000000
VERSION     DATNOI010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2098274"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..602
                     /locus_tag="VN968_03670"
     CDS             195..602
                     /locus_tag="VN968_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXL28376.1"
                     /translation="MVASKMALVMVLTAIANGPVRAESGLASFYGGGRTASGEVTGPA
                     GLTAAHRTLPFGTMVVVTNLRSGNSVIVRITDRGPYRHRRIIDVSRAAARELGMINSG
                     TAMVSILPQQRADRPICTGGAWLKGLQLLSSPR"
     gene            720..920
                     /locus_tag="VN968_03675"
     CDS             720..920
                     /locus_tag="VN968_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28377.1"
                     /translation="MNGNLAYYVGIDRENNTMTNVSVWASLDDAMQMASLPAILALAR
                     TFAEMGVRFETPITNHETLWSI"
     gene            1246..2745
                     /locus_tag="VN968_03680"
     CDS             1246..2745
                     /locus_tag="VN968_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXL28378.1"
                     /translation="MPDADVLIAGAGPTGLMLASELALAGIDVAIVERRASQELAGSR
                     AGGLHARTIEVLDQRGIADRFLSEGQVAQVAGFAGTRLDISDFPTRHNYGLGLWQNHI
                     ERILAGWVAELAVTIYRGREATGFTQDETGVDVALSDGQSLRADYLVGCDGGRSLIRK
                     AAGIAFPGWDPTTSSLLAEVEMAEQPELGIHRNAFGVHSFGKVKYEIRDGEIVYMDGG
                     PVGVMVTEQHIGPTTEPTLRDLSEALIAVCGTDYGIHSPTWVSRFTDMTRQAASYRKG
                     RVLLAGDAAHVHAPDGGQGLNIGVQDAVNLGWKLAQVVKGTSPESLLDSYHAERHPIA
                     ARVLRNTMASVVLRRPDERTKALRDTMSELLGMDEPRRRFGAMMSGLDIHYDLGEGHP
                     LLGRRMPDLDLVTADGPLRVFTLLHDARPVLLNLGEPGGFDITPWADRVHLIDAKCVG
                     RWELPALGVVTAPTAALIRPDGHVAWVGDHADLGLPDALTTWFGPPTAA"
     gene            3090..5063
                     /locus_tag="VN968_03685"
     CDS             3090..5063
                     /locus_tag="VN968_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HXL28379.1"
                     /translation="MDLPSSAPVGGLIDRSVTNAGPSRSEAVLDQAKYLHHAGSPWFL
                     ALTALGVVFGDIGTSPLYAFQVALTGVGHSPPTQADVLGIVSLILWALTVMVSLKYVV
                     FVLRADNDGEGGILALLSLVAADQVANGARLPVLVLLGVIGASLLYGDGVITPAISVL
                     SAMEGLKLVAPGFEHFVVPATIAVLIGLFVIQRYGTGSIGKLFGPIMVIWFVVIGGLG
                     AANIWMAPAILRAVSPTEAVSFLIADPKISFVVIGAVFLALTGGEALYADMGHVGANA
                     IRRAWFGLVLPALLLNYFGQGALILSDPAAADNPFYKLAPGWALIPMVVLATFATIIA
                     SQALVSGVFSLTRQAMQMGLCPRARITPTSVDEAGQIYVPAANWLLMTGTLLTVVLFR
                     SSENLAAAYGIAVSGTMLITTILLYRVAVSRWQWPPAVAIPVIAIFGAIDATFLVSNS
                     IKIVEGGWFPLIVGGLIAVLMLSWRKGSSEVRHRLHEMSMPLNEFIEYADNTCIGRAP
                     GMGVWLTKVEHGASPMLLRHIEHNRVLHETVVLLTFVSDRRPRVPFHERHSVHRLGHG
                     FYRIQVRLGFMQTPDIPLTLINCNRLGFDADLDHKNYYIAHETIVRRAVGSAIEPIPF
                     AIFSFLNRIASRAPDFFKIPHDAIIEVGFRVEV"
     gene            5269..5454
                     /locus_tag="VN968_03690"
     CDS             5269..5454
                     /locus_tag="VN968_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28380.1"
                     /translation="MTGDQSRGPKIGARVCWGEDKDDQATVTEKNWSGVTLKWDNRDN
                     QSVSHNDMKMVFLVAEK"
     gene            5459..5623
                     /locus_tag="VN968_03695"
     CDS             5459..5623
                     /locus_tag="VN968_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28381.1"
                     /translation="MKRPNPKEAGTEVDCPACDGTGFPAVTQPEQPGRKIFPARCQQC
                     LGKGRIDTLS"
     gene            complement(5769..6290)
                     /locus_tag="VN968_03700"
     CDS             complement(5769..6290)
                     /locus_tag="VN968_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28382.1"
                     /translation="MTRTEARQHYGSAHLYWHGADHCWTATAANQHHRIHYVREKSTH
                     HVREQADHRDREKSDRQARPKTDHQAAEPKIDHPKWHESMSQMLPGDEEPVRPSWVER
                     WVDIEPAQSPLGARWVDIVQAAPADIMAAESEPMITPRSVMMVITVLTLTLAIVEFLI
                     IGMRLAGPPWRWL"
BASE COUNT         1463 a         2355 c         2457 g         1572 t
ORIGIN      
        1 atgatgagag gggccatgga aactccgaac aggcaattcg aaaagatggt atcgcagcgt
       61 ccgcggcgac ctttcccggc agcctagctt gcaattctcg cggaagcagc gaaaattttg
      121 ccagattgcg ccgtgcagtc gctcaatccg ccagtccagc gggaacccag tagatcatcc
      181 ggcgttcatg acggatggtt gcctccaaaa tggcattggt gatggttttg actgcgatag
      241 cgaatggtcc cgttcgcgct gaaagcggtc ttgcctcatt ttatggcggc gggcgcaccg
      301 caagcggcga agtaaccggc ccagcggggc tgacagccgc tcatcgcacg ttgccttttg
      361 gcacgatggt agtggttacc aaccttcgca gcggcaactc ggtgatcgtg cgcatcacgg
      421 atcgcggccc gtatcgccat aggcgcatca tcgacgttag ccgcgccgcg gcccgcgaac
      481 tcggcatgat caacagcggc acggcaatgg tcagcatcct gccccaacag cgtgccgaca
      541 ggccaatctg tacaggcggg gcttggctca agggcctgca attgctcagt tcgccgcgat
      601 gagcaggagt cccgcaaatg acggttgtcc gaatttctct tgaccgcttc gatgcgtcaa
      661 attaccggat tgcccaacgt tgcttgatga aagccaggct tcgctgaagc tggcaatggt
      721 tgaacggcaa tctggcctat tacgttggaa ttgaccggga aaacaacacc atgaccaacg
      781 tcagcgtgtg ggcatcgctt gatgatgcga tgcaaatggc ctcgcttccg gcgatacttg
      841 cgctggcccg aacctttgca gaaatgggcg tacgtttcga aacacccatt accaaccatg
      901 agacgctttg gtcgatttga gacgagcgct tttgctgatc gtcattgagc cgtgtgccgg
      961 ccggggcgta tcgggtcgct aaatgcctgg tccgatgcga cgaggatatt cattagggcg
     1021 tggacaaatt aacgcgcagc catagcctga tcgacgcaag ctggatgaac gcaaggtaga
     1081 ccgcctgaca tggatatggg gccgaactaa agaagttcaa gaattgcaaa gcttcgattt
     1141 gcagccttat caaatccctt cagaaacacg cacgtgctca tctcttggtg gggatgcggg
     1201 cgggctgaag ccagccattg cgaacgacgg gagcgtttga tgaacatgcc ggatgccgat
     1261 gtcttgatcg cgggtgcagg cccgaccggg ctgatgctgg cgagcgagtt ggcactggcg
     1321 ggcatcgacg ttgccattgt cgagcggcgc gccagccagg agctcgccgg ctcgcgcgcg
     1381 ggcggtctgc atgcccgcac gatcgaggtt ctcgatcagc gcggaatcgc cgatcggttc
     1441 ctgtcggagg gacaggtggc tcaggtcgca gggttcgccg ggactcggtt ggacatcagc
     1501 gactttccta cccgacacaa ctacggcctc gggttgtggc agaaccacat cgaacgcata
     1561 ctggctggct gggtggcgga gctggcggtg acgatctatc gcgggcgtga ggcgaccggt
     1621 ttcactcagg acgagactgg cgtcgacgtc gcgctgtccg acggccagtc gctgcgggcg
     1681 gactatctcg tcgggtgcga cggcggacgc agcctgatcc gtaaggcagc cggcatcgcg
     1741 ttccccggat gggatccgac gacgagcagt ctgctcgccg aggtcgagat ggccgagcag
     1801 ccggaactgg gcatccaccg caacgccttt ggcgtccatt ccttcggcaa ggtgaagtac
     1861 gagatccgcg acggcgagat cgtctacatg gatggggggc cggtgggggt catggtgacc
     1921 gaacagcaca tcggacccac gaccgaaccc accctgcgcg atctcagcga ggcgctcatc
     1981 gccgtgtgcg ggacggatta cgggatccac agtcccacct gggtctcgag gttcaccgac
     2041 atgacccggc aggcggcgtc ctaccgcaag ggacgggtgc tgctggccgg cgacgccgcg
     2101 cacgtgcatg ccccggacgg tggacagggc ctcaatatcg gtgtgcagga cgcggtgaat
     2161 ctgggatgga agctggccca ggtggtcaag ggaacgtcgc cggaaagcct cctggatagc
     2221 taccacgccg agcgccaccc gatcgctgcc cgcgtgttgc gcaacacgat ggcctcagtc
     2281 gtgcttcgcc gcccggacga acgcacgaag gccctgcgcg acacgatgtc cgagctcctc
     2341 ggcatggacg agccgcgcag acggttcggc gcgatgatgt ccggtctcga cattcattac
     2401 gatctcggcg agggacatcc gctgctcgga cgccgcatgc ccgatctcga tctggtcacc
     2461 gccgacggcc cgctgcgggt cttcaccttg ctgcatgatg cccggccggt gctgctcaac
     2521 ctcggtgagc ccggcggctt cgacatcact ccgtgggcag atcgggttca tttgatcgac
     2581 gccaaatgtg ttggtaggtg ggagcttccg gcactcgggg tagtcaccgc tcccaccgcc
     2641 gcgttgatcc ggcccgatgg acatgtcgcc tgggtaggag accacgccga cctgggcctc
     2701 cctgacgcgc taaccacctg gttcggaccg cctaccgcgg cgtagcgcac cggcttccgg
     2761 ttcagccaaa gcctatactc tgccgtagtg cttgttgtga gattgctgga tcgtcttggc
     2821 actccaggtg gcaaacgcgt tgccgggagt tgctcatgat cgcggttcaa aaaacttcgt
     2881 ctttgccgaa gtgtcttcgt agcaattaat ttgcgacatc ctccgttctt gcggcaattc
     2941 ggtcgctttc gcatgaacag aggcggctgt tgcggcgccg cgacggccat ccccgggatg
     3001 gcgccttccg gtcggccgag ttccgtgaca tggcctaaat cttgaatgcc tgaactcgcg
     3061 agcgcacaca atgtggtcgg gaggcggaca tggatttacc atcgagtgcc ccggtcggcg
     3121 gcttgatcga ccgttcggtg acgaatgccg gaccaagccg atccgaggct gtgctcgatc
     3181 aggccaaata ccttcatcat gccggcagcc cctggttctt ggcactcacc gcactcggcg
     3241 tcgtgttcgg cgacatcggg accagcccgc tctacgcttt ccaagtggcg ctgaccggcg
     3301 tcggtcattc gcctccgaca caagccgacg ttctcgggat cgtctcgctg attctctggg
     3361 cgctcacggt gatggtgtcg ctgaagtatg tcgtgttcgt gctgcgcgcc gacaatgacg
     3421 gcgagggcgg catcctggca ttgctgtccc ttgttgccgc cgatcaggtc gccaacggcg
     3481 caagacttcc cgtgctggtt ctgctcggcg tgatcggcgc ctcgttgctc tatggcgacg
     3541 gcgtcatcac gccggcgatc tcggtgcttt cagcgatgga agggctgaag ctggttgcgc
     3601 cgggatttga acatttcgtc gtgccggcca cgatcgctgt gctgatcgga ctgttcgtga
     3661 tccagcgcta cggcaccggc agcatcggaa aactgttcgg cccgatcatg gtgatctggt
     3721 ttgtcgtcat cggcgggctg ggcgccgcca atatctggat ggcgcccgcc attctcaggg
     3781 cggtcagccc aaccgaggcc gtgagctttt tgatcgctga tcccaaaatc tcgtttgtcg
     3841 tgatcggcgc cgtattcctg gcgctgaccg gcggcgaggc gctgtacgcc gacatgggcc
     3901 atgtcggcgc caacgccatc cgccgcgcct ggttcggcct ggtactgccg gcgctgcttt
     3961 tgaattattt cggccagggc gcgctgatcc tcagcgatcc cgcggcggcc gacaatccct
     4021 tctacaaact ggcgccgggc tgggcgctga tcccgatggt cgtgctggcg accttcgcca
     4081 ccatcattgc gtcacaggcg ctggtctccg gcgtgttttc gctgacgcgg caggccatgc
     4141 agatgggatt gtgcccgcgg gcgaggatca cgccgacgtc ggtcgatgag gcagggcaga
     4201 tttacgtgcc cgccgctaat tggctattga tgaccggcac gttgctgacc gtggtgctgt
     4261 tccggtcgtc ggaaaatctg gcggcggcct atggcattgc agtatccggc accatgctga
     4321 tcaccacgat ccttctttac cgcgtggcgg tgtcgcgatg gcagtggccg ccggccgtcg
     4381 cgatccctgt catcgcgatc ttcggcgcga tcgatgccac cttcctggta tctaattcga
     4441 tcaagatcgt cgaaggcggc tggtttccgc tcatcgtcgg cgggctgatc gcggtgctga
     4501 tgctgtcctg gcgcaagggc tcttcggaag tcaggcatcg tctccacgag atgtcgatgc
     4561 cgctgaacga atttatcgag tatgccgaca atacctgcat cggccgcgcg ccgggaatgg
     4621 gggtatggct caccaaggtc gagcacggcg cctcgccgat gctgctgcgt catattgagc
     4681 acaaccgggt cttgcatgaa accgtggtct tgctgacgtt cgtgtccgat cgccggccgc
     4741 gggtgccgtt tcacgagcgt cattcggtgc atcggctggg ccacggtttt tatcgaatcc
     4801 aggtcaggct gggatttatg cagacgccgg atattccgct gaccctgatc aactgcaaca
     4861 ggctcggctt cgacgccgac ctcgaccaca agaactacta catcgcccac gagaccatcg
     4921 ttcgccgcgc cgtcggatcg gcaatagagc cgatcccttt tgcgattttc tcgttcctga
     4981 atcgtatcgc gagccgcgcc ccggatttct tcaagattcc ccacgacgcc atcatcgagg
     5041 tgggatttcg ggtcgaggtc tgacgcgggc ggcgttggcg gaatgcgcgc cgctgcgcgc
     5101 caaaacgcgg gatttgttgg ggcgccggta accgacagcc aatcgacacg tagtagtacg
     5161 ggactctatc ttaaccgcaa cttgggatgg ttggccggcg cggcttttta gggtcgccgt
     5221 ccaccgggcc gcacactatt gttatcgtca tccaatggag ctgcgacaat gaccggcgat
     5281 caatcccgag gacccaaaat aggcgctcgc gtctgctggg gcgaagacaa ggatgatcaa
     5341 gccaccgtca ccgaaaagaa ctggtccggc gtaactctca aatgggacaa tcgtgacaat
     5401 caatccgttt cgcataacga tatgaaaatg gttttcctcg ttgcagagaa atgagttcat
     5461 gaagagaccc aaccccaagg aagcagggac agaagtcgat tgcccagcct gtgacggcac
     5521 gggatttcca gcggtcaccc aaccggagca gcccggtcgc aagatcttcc ccgcgcgctg
     5581 ccagcaatgt ctcggcaagg gaagaataga tacgctcagc tgatagggcg gatttgccat
     5641 cgggtcgcgc gaatgcgcgc tcaatgataa tctctgggtc attcgccgtt cagcgtcgac
     5701 aaagccgttg ggttatgcgt tgccaacccg ccctggggcc tattctcacg cgggccaact
     5761 gctccgggtc atagccagcg ccagggcggt ccggccagtc tcatgccgat gatcagaaac
     5821 tcgacgatcg ccagcgtcag ggtcaggacg gtgatcacca tcatcacgct gcgcggcgtg
     5881 atcatcggct cggattcggc tgccatgatg tccgccggcg cggcctggac gatgtcgacc
     5941 cagcgcgcgc caagggggga ctgcgccggc tcgatatcca cccagcgctc gacccacgat
     6001 ggccgcaccg gttcttcatc gccgggcaac atctgcgaca tcgattcatg ccatttggga
     6061 tgatcgattt ttggctcggc ggcctgatga tcggtttttg gccgcgcctg acgatcggat
     6121 ttttcccgat cccgatgatc ggcttgttcc cgaacgtgat gggtggattt ttcccgaacg
     6181 taatggatcc ggtgatgctg gtttgccgct gtcgcggtcc agcagtgatc ggcgccatgc
     6241 caatagaggt gcgccgaacc ataatgctgg cgcgcttcgg tcctggtcat gcaggacggt
     6301 gaagcctctg aggctgtagc caatatcgac acaaacacaa caaatagcgc gacacgcatc
     6361 ggtcgctcct gaaatacgct ttactcaacc aaatcgaatc actggggtca gctttggctg
     6421 acttcaaggc gaaatttgga agcccgcgag ccggaccggc taccgcgtcg cgcgaaagcg
     6481 cacgcgacaa gtaaactccg cggcacccat caattgcatt tatgcagagc gcccgcgctg
     6541 atcgttctcc ggatgcagcg cagcgccacc agcgcgttta cgcgcgtctt tgacgcgcca
     6601 tcagcgcgtt tacgcgcgtc tttgacgcgc aatgtcggcg tggtgcgctg ctgatccggg
     6661 ggccagcgtg ccgttggctc cgctgggttc cggctctgcg gagcagcgtg aagggcgctg
     6721 caccgcgtcc gggacacgag aggaaatcgc gcttcgcggc cgtgtggtga tttgcccgtc
     6781 gggcgggcgg tggacagggg tgtccagccc cctttgcaaa aatattttgg ttttcacacc
     6841 ccccaaatca cttctagaac ttttcgtgtc ccaccccaaa agaggggcgt gtcatgatcg
     6901 tcacgaacgc ggggtgggat gcggtggacg cggcagcgtt ttgcgcgcga cgggatcgca
     6961 gggcgggttc tcaggaatcc gtgagcgatc aacagcgcgc tgacgagaga cgcttttcgc
     7021 ggacggcaaa atcgtgtggt cctgacgccc cgacgctggc gtcaagcttt gcggagttct
     7081 gtcggcccaa ccgggcaatg acaaaacgat atccgcgaag cgacggtggc aaaagagccc
     7141 ggtcaccggg gagagcacga cataagccgt aaaaccattg cgtgcgggaa tgccgggtga
     7201 ttccggtgta ctcgttgtta ctcgtgtgcg ttctaccaat acaaagtgca cacgaaaccg
     7261 cgggtgcagc gggcatccgg cattccccac gccctcaagg ggcgagagat aaatgcacga
     7321 cctgggcgca tcgcgccgcg gggtcgcgaa gtcgcgtctg gaattgtttt agcgagcaac
     7381 aagctctacg tcgtcccgaa caagcgaagc gcgatccggg atccataacc accaaggtcc
     7441 atgttgtgca aagctggggg ccacgattcc atccatcgct agcattggtg gcggtcccgg
     7501 ccttcgccgg aacgacgaaa gagtccgaat gggctttccg tcgttgcgag tcgcaacgcg
     7561 acgaatccag acgccgtcat tccgggttcg cgctggcgcg cgtcccggaa tgacggttat
     7621 gcatttacgc gcggagcggc aacggttttt ggtcgctcaa cccttgcgcc ggcccatcat
     7681 cggcggcaca tttggcgcgc ggcacacatg gccgccaaga acgacatcct agggaaaacc
     7741 catggcggcg acgcggatcg actgcgatat ccatcccgcg gtgggcggaa cccgcaccac
     7801 gctgctgccc tatctcgacg atcactggaa ggaacaggtg gtgagcc
//
LOCUS       DATNOI010000106         6860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1724435,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000106 DATNOI010000000
VERSION     DATNOI010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724435"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VN968_03705"
     CDS             complement(<1..381)
                     /locus_tag="VN968_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HXL28383.1"
                     /translation="MAPGFSDIVEETRLSARALLEYADHFFHPTVRLGVTGLSRAGKT
                     VFITALIHGLTRGGRFPIFEPFATGRIARARLEPQPDDAVPRFDYENHVRTLIEERRW
                     PNSTVDISELRLAIDYQRQNGADRT"
     gene            565..1260
                     /locus_tag="VN968_03710"
     CDS             565..1260
                     /locus_tag="VN968_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HXL28384.1"
                     /translation="MTVFRLKQLASSSAHAAAGAIRWNYDRAELIADGIVHGIGVFGG
                     VVAATVLIVLAAIYASPFEVAVVSVYVAGLLAMLVLSATYNLWPISRAKWLLRRFDHS
                     AIYVLIAATYTPFITQLKDSIFAVALLIGVWCVAIVGAALKLVLPGRFDRLSVGLFLA
                     MGWSGAMLYDAVVAALPAMALWFVIAGGALYSLGVIFHAWQRLRFQNVIWHCFVLLGA
                     ACHYTAVLDLVLT"
     gene            1292..2086
                     /locus_tag="VN968_03715"
     CDS             1292..2086
                     /locus_tag="VN968_03715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL28385.1"
                     /translation="MQVTGKIVVVTGGAKGIGRALCDVFHRAGAAKVVVADIDPAGAR
                     AVAAKVDGAAFKCDVGKETDIRHVVEETERQFGPIALFCSNAGIGGGFDPLADNAGGT
                     SDEPWSRSWAVHVMAHVYAARHLVPRMKARGEGYFLNTISAAGLLSQIGSPAYSTTKH
                     AAVGFAESLAISHKAHGIRVSILCPQGVDTDMLRSIPKGPQSGDGDLTPEQVANDALQ
                     GIEQETFAILPHPQVLGYMRKKTENYDRWISGMAKIQARMREDYGK"
     gene            2229..2609
                     /locus_tag="VN968_03720"
     CDS             2229..2609
                     /locus_tag="VN968_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28386.1"
                     /translation="MEFKQRTLMQIADMICGNPKSPEEKCLFRYQSSSRLTEFFQDCD
                     TDYRHDGSTRNYWVAETLKSILKEPQASANAPPETFARVIRVLKDQADAENEEPPRSG
                     ALTILNAALAREGFERRKSRGVNT"
     gene            2657..3130
                     /locus_tag="VN968_03725"
     CDS             2657..3130
                     /locus_tag="VN968_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28387.1"
                     /translation="MLPLFRQLGFHRVTAAGHMDKSLEYGKDVWMRFTLPTQHVLYFG
                     IQAKKGKLDAAGAGKPGNVNVAEIHNQTMMMLGHEIFDPEIGKRVLVDHAFIVAGGEI
                     TKAARNWLGNKLDATKRSQVMFMDRDDILNLFVVTNLPLPTGALPAAEPDDDLPF"
     gene            3477..3968
                     /locus_tag="VN968_03730"
     CDS             3477..3968
                     /locus_tag="VN968_03730"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL28388.1"
                     /translation="MSNTAPSAPEVAQLKVNEKKWSAPLMKAGWTALPNVIFERQRAL
                     GLDAIDVNIILHIASYWWTAEGKPFPSKKTIATAMNIDPRTVQRRIAAMETAKLIKRE
                     ERRTSAKGSDTNIYHLDGLIEAAKPFALEKIQERDAKAQLRAARAARKGKPKLTVVKN
                     GGA"
     gene            3975..4078
                     /locus_tag="VN968_03735"
     tRNA            3975..4078
                     /locus_tag="VN968_03735"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(4263..5888)
                     /gene="mdlC"
                     /locus_tag="VN968_03740"
     CDS             complement(4263..5888)
                     /gene="mdlC"
                     /locus_tag="VN968_03740"
                     /EC_number="4.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoylformate decarboxylase"
                     /protein_id="HXL28389.1"
                     /translation="MSRKPAKSAHASISVKDATLNLLRAFGIKKVFGNPGSTELPFLS
                     DWPDDIDYVLGLQEASVIGMADGYAHATRNAGFVNLHSAAGVGNALGNIYTAHRNQTP
                     LVITAGQQARSILPLQPFLYAERASEFPRPYVKFSVEPARAEDVPAAIARAYYVAMQP
                     PCGPTFVSVPVDDWTHQTQAIDARDVSREIGPEAGAMKTLAAALAASKCPALVVGPAV
                     DRAQAVDLMVRLAEKAKAAVWVSPFSARCSFPERHPQFAGFLHASPAQLSEALRGHDL
                     VVVIGAPVFTFHVEGHASIFDGATSIFQITDDPTAAAVTPQGASIIATMKPALTMLID
                     LLPESRRAMPAGRALSAAPRAADPIPVDFLLHTLSQAMPDDAALVEEAPSHRPAMQNF
                     MPMRGEDSFYTMASGGLGYSLPASVGIALGRPNIRTVCLVGDGSAMYSIQALWTAAQR
                     KLPLTVVVINNAGYGAMRSFSQVMQVRNVPGLDLPGIDFVLLAQGLGCDAVRVTRSSE
                     LAPALKRGLAHHGVSLIEVIVDSAVPVLYAQKS"
     gene            complement(5976..6830)
                     /locus_tag="VN968_03745"
     CDS             complement(5976..6830)
                     /locus_tag="VN968_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HXL28390.1"
                     /translation="MTDKPDLDHLRQWIGRTDEASDIVTAQLVKGLRATLFLEIGKPK
                     AGDTAPFTAHWCLAQPVYPMSELGPDGHPARGGFLPPVPLPRRMWAGGEVEFFDTLRV
                     GDEMTRTSRIADVTMKTGSTGALCFVSVDHRIATPRGTAIRERQDIVYRGNSPAPPSV
                     SARPPAPPPAAQHRESHMADAVLLFRYSALTFNGHRIHYDRDYVTRIEGYPGLIFHGP
                     MQAALLVEFAAKLRRGAAPKKFSYRGVQPLFEGSEFSTNANDTDAGMELWTANSEGQP
                     TMKGTATW"
BASE COUNT         1359 a         2042 c         2157 g         1302 t
ORIGIN      
        1 cgtgcgatcc gcgccattct gccgctggta gtcgatcgcg agccgcaatt cgctgatatc
       61 gacggtcgaa ttcggccagc gccgctcctc gatcagggtg cgcacgtggt tctcatagtc
      121 gaagcgcggc accgcgtcgt caggttgcgg ctcaagccgg gcgcgagcaa tccggccggt
      181 ggcgaaaggt tcgaaaatgg gaaaacgacc gccgcgggtg aggccgtgaa tcagcgccgt
      241 gatgaagacg gtcttgccgg cgcgtgacaa tccggtaacg ccgagcctga ccgtcggatg
      301 gaaaaagtgg tcggcatatt ccagcagggc ccgcgccgac aggcgcgttt cctcgacaat
      361 atcggaaaaa cctggggcca tacgggcgcg gaggactcga ttttagggga ggaacaattg
      421 gcgagagcta tccggaaagt ggcgattagc gggcagaaat caagtttcat acttacgacg
      481 cgatttgagg cgaggagttt cagggttcgt tgacctcccc cggggtatgt tttaactctt
      541 actaccaagc ttgccggaac cgccatgacc gtcttcagac taaagcaact cgcctcgagc
      601 tccgcccatg cggcggccgg cgccatccgc tggaattacg atcgggccga actgatcgcc
      661 gatggcatcg tacatggaat tggcgtgttc ggtggagtgg tcgcggccac ggtgctgatc
      721 gtgctcgccg cgatctatgc ctcgccgttt gaagtggcgg ttgtctcggt ctacgtcgcc
      781 ggattgctgg ccatgctggt attgtcggcg acctacaatc tatggccgat ctcgcgggcc
      841 aaatggctgc tgcgccgctt cgatcactcc gcgatctatg tgctgatcgc ggcgacctac
      901 acgccgttca tcacgcaatt gaaggacagc atcttcgccg tcgcgctgct gatcggcgtc
      961 tggtgcgtgg cgattgtcgg ggcggcgctc aagctggtgt tgccgggccg tttcgaccgg
     1021 ctctcggtcg gcctctttct ggcgatgggc tggagcggcg ccatgctcta tgatgctgtc
     1081 gtggcggcgc tgccggcgat ggcgctctgg ttcgtgatcg cgggtggcgc gctgtatagt
     1141 ctcggtgtga tctttcacgc ctggcagcgg cttcgcttcc agaacgtgat ctggcattgt
     1201 ttcgtgctgt tgggcgcggc ctgccattac acggcggtgc tcgacctcgt cctgacctag
     1261 agatcggatt gcaaaaaagg ctgaggtagc ggtgcaggtg acgggcaaaa tcgtggttgt
     1321 caccggcggc gccaaaggca tcgggcgcgc gctctgcgat gtctttcatc gcgccggtgc
     1381 ggccaaggtg gtcgtcgccg atatcgatcc ggccggagcg agagcggtcg cggccaaggt
     1441 cgacggcgcg gcgttcaaat gcgatgtcgg aaaggagacc gacatccgcc acgtcgtcga
     1501 ggaaaccgag cggcagttcg gcccgattgc gctgttctgc tccaatgccg gcatcggcgg
     1561 cgggttcgat cccctggcgg ataacgccgg cggaacttcc gacgagccct ggtcgagaag
     1621 ctgggcggtc catgtcatgg cccacgtcta cgctgcgcgg catttggtgc cgcgcatgaa
     1681 ggcgcgcggg gaaggttatt ttctcaacac catttcagct gccggactgc tgtcgcagat
     1741 cggaagtccg gcctattcga ccaccaagca tgcggcggtg ggctttgccg agagccttgc
     1801 gatctcgcac aaggcgcatg gcatccgggt ttccatcctg tgcccgcagg gggtcgatac
     1861 cgacatgctc cgctcgatcc cgaaaggccc gcaatccggc gatggcgatc tgacgccgga
     1921 gcaagtcgca aacgatgccc tgcagggaat cgagcaggaa acatttgcca ttttgccgca
     1981 cccgcaagtg ctcggctaca tgcgcaagaa gacggaaaat tacgatcgct ggatatccgg
     2041 catggccaag atccaggcca gaatgcgcga agattacggg aagtaaagag gaccctcatg
     2101 gtgaggagcg cggtacgcgc gtctcgaacc atgagttccg tttttcggaa tgcgcgtcca
     2161 agtgccggcg tgctacattc gcgattacgc catcaatttt ggtggcgaac catagcttca
     2221 ttgaatccat ggaatttaag cagcgcacac tcatgcaaat cgcagacatg atttgcggca
     2281 atcccaaaag tcccgaagag aaatgcctgt ttcgctacca gagcagcagc cgtctcacgg
     2341 aattttttca agactgcgat accgactaca gacacgatgg ctcgacccgg aattattggg
     2401 tcgctgaaac cctcaaaagc attttaaagg agccgcaggc aagcgcgaat gcgcctcccg
     2461 aaaccttcgc acgcgtcatc cgcgtactca aggaccaagc ggatgcagaa aacgaagaac
     2521 cgccgagaag cggtgcgctg acgattttga atgcagcact cgctcgcgag ggatttgagc
     2581 gcaggaaatc gagaggcgtg aacacctgat tgcttacctt gacaaggcct cagaggacga
     2641 actgattgcg gaggtgttgc tgccgctgtt tcggcagctt gggttccatc gcgtgaccgc
     2701 cgctggacac atggataaat cgttggagta cggcaaggat gtttggatga ggttcacgct
     2761 gcccacccaa catgtccttt actttggcat tcaggcgaag aagggcaaac tggacgcggc
     2821 cggcgccgga aaacccggca acgtcaacgt tgcagagatt cataatcaga cgatgatgat
     2881 gcttgggcac gaaatttttg atccggagat tggtaaacgt gtccttgtcg atcacgcctt
     2941 catcgttgcc ggcggcgaga ttacaaaggc ggcccgaaat tggctcggca ataagctgga
     3001 cgccactaag cgcagccaag tcatgtttat ggaccgcgac gatatcttga atctgtttgt
     3061 ggtcaccaac ttgccgctgc cgactggcgc ccttccagcc gcagagcccg atgatgatct
     3121 gccgttttag ctggagcgat cacatcaacg cggcctaaac cgccgcgcta cgctgcctca
     3181 tgcgccttaa agtgttcctt gggggctcaa ggcacttttt gtcgatgtgc ccctagggtc
     3241 cttgtgtctc ccccgggagg cacctacatc cggggaggga gacttcagac gctcccgtcc
     3301 cccgggcggg tgtgccctac taataagtac gcagcctata cacacgcaca tcctatacgc
     3361 ccattcagca ttccaatatg tatagggagg gcgaccccag ggtctccccg gtcgatttgc
     3421 cttcccgcgc aacgtgctca tattggtgac agttcgaaat tcacggagaa cattcaatgt
     3481 caaacacagc accgagcgcc cccgaggtcg cacaactcaa ggtcaatgaa aagaagtggt
     3541 cagcgccgct gatgaaagcg ggctggaccg cgctgccgaa cgtgattttt gagcgtcagc
     3601 gggccttggg actcgatgcc atcgacgtca acatcatcct gcatatcgca tcttactggt
     3661 ggactgctga aggcaagccg ttcccttcca agaaaaccat tgccacagca atgaatattg
     3721 acccgcgaac agttcaacgc cgcatcgcgg cgatggagac cgccaagttg attaaacgag
     3781 aggagcggcg tacctccgca aagggcagcg acaccaacat ctatcacctc gacggtctga
     3841 tcgaagcagc gaaaccgttt gcgcttgaga agattcagga gcgggacgcc aaggctcagc
     3901 tccgagcagc cagagcggcc cgcaagggca agccgaagct taccgttgtc aaaaacggag
     3961 gcgcctaacc gttccgcgct gtagcttaat cggaagagca cctgccgtct gtgaaatcct
     4021 aggctgacac ctggagagta agggcaggaa gatgggggtt caagtcccgc cagcgcgcca
     4081 aatcagccag atgtgggcgg cttcttgccg aagtgatcca tatcgggtct gtcagaatga
     4141 tttgtgtgtg agagataata accgcggggc accaacgaaa caccgaggga gcgaaatcct
     4201 aggttggcgt cacgctcgct cccagatgtc gcttcgctcc atccggttac gatgttttcc
     4261 gctcagctct tctgcgcgta aagcactggc accgccgaat cgacgatgac ctcgatcagg
     4321 ctgacgccgt gatgcgccag gccgcgcttg agagcgggcg cgagttcgga tgatcttgtc
     4381 acgcgcaccg catcgcagcc gagcccctgc gctaacagga cgaagtcgat gccgggcaga
     4441 tcgaggcccg gaacattgcg cacctgcatc acctggctga acgagcgcat cgcgccatag
     4501 cctgcgttgt tgatcaccac gaccgtcagc ggcagcttgc gttgcgccgc ggtccatagc
     4561 gcctggatcg aatacatcgc ggaaccgtcg ccgaccaggc aaacggtcct gatgttgggg
     4621 cggccgagtg cgatgccgac cgaggccgga aggctgtaac cgaggccgcc gctcgccatg
     4681 gtgtaaaaac tgtcttctcc gcgcatcggc atgaaatttt gcatcgccgg ccgatgcgac
     4741 ggggcttcct ccaccagcgc ggcgtcgtcg ggcatggctt gcgaaagcgt gtgcaacagg
     4801 aaatcaacgg ggatcggatc ggccgcgcgt ggggcggcgg acaatgcgcg gcccgccggc
     4861 atcgcgcgcc tgctctcggg caggagatcg atcagcatcg tcagcgccgg cttcatggtc
     4921 gcgatgatgc tggcgccctg cggcgttacc gccgccgccg tcggatcgtc ggtgatctgg
     4981 aagatgctgg tcgcgccatc gaagatcgag gcatgacctt cgacgtggaa cgtgaacacc
     5041 ggcgcgccga tcaccaccac cagatcatgg ccgcgcaacg cctccgagag ctgagccggg
     5101 gaggcatgca gaaaacctgc gaactgcgga tgccgttccg ggaaactgca gcgcgctgaa
     5161 aacgggctga cccacaccgc cgctttcgcc ttctccgcaa ggcgcaccat cagatcgacg
     5221 gcctgcgccc gatcgacggc ggggccgacc acgagggcag ggcacttgct tgccgcgagc
     5281 gccgcggcga gtgtcttcat tgcgcccgcc tcggggccaa tctcgcggct gacgtcgcgg
     5341 gcatctatcg cctgcgtttg atgggtccag tcgtccaccg gcaccgacac gaaagtcggt
     5401 ccgcacggcg gctgcatcgc cacgtaataa gcgcgcgcga tggccgccgg gacatcttcg
     5461 gcgcgcgccg gctcgaccga gaacttgaca tagggccgcg gaaattcgga cgcgcgctcg
     5521 gcatagagaa atggctgcag cggcaggatg cttcgcgcct gctggcccgc ggtaatcacc
     5581 agcggcgtct ggttgcggtg ggcggtatag atattgccga gcgcattgcc gacgccggcg
     5641 gcggaatgca gattgacaaa gccggcgttg cgggtggcat gcgcatagcc gtcggccatg
     5701 ccgatcacgg aagcttcctg caggcccagc acataatcga tatcgtcggg ccaatcgctc
     5761 aggaacggca actccgtcga gccgggattg ccgaacacct ttttaatgcc gaacgcccgc
     5821 aacaggttga gcgttgcgtc cttgacgctg attgatgcgt gagcgctctt tgctggcttg
     5881 cgggacaatg cggcgtcctt ggaggattac tcacccgtta ttgcgaggag ccaacgggtc
     5941 gcgcgttcgc gcgacccgtt ggctcgcaat gacgatcacc acgtcgcggt gcccttcatg
     6001 gtcggctggc cttccgaatt cgcggtccac aactccatgc cggcatcggt atcgttggcg
     6061 ttggtggaaa attcgctgcc ttcgaacagc ggctgcaccc cgcgatagct gaatttcttc
     6121 ggcgccgcgc cgcgacggag ttttgccgcg aattccacca gcaacgccgc ctgcatcggg
     6181 ccgtggaaga tcaggcccgg atagccctcg atcctggtga cgtagtcgcg gtcgtaatgg
     6241 atgcggtggc cgttgaatgt cagtgccgaa tagcgaaaca gcagcaccgc atcggccatg
     6301 tggctttcgc ggtgctgcgc ggccggaggc ggcgctggcg gcctggctga caccgatggc
     6361 ggcgcagggg agttgccgcg atagacgatg tcctgccgtt cccggatcgc ggtgccgcgc
     6421 ggcgtcgcga tccggtgatc gacggataca aaacacaacg cgccggtaga gccggtcttc
     6481 atcgtcacat ccgcgatgcg cgaagtccgc gtcatctcgt cgccgacgcg caacgtatcg
     6541 aaaaattcca cctcgccgcc ggcccacatc cggcgcggca gcggcaccgg cggcaggaag
     6601 ccgccgcgcg cggggtggcc gtccggtccg agctccgaca tcgggtaaac cggttgcgcc
     6661 aggcaccaat gcgcggtgaa gggcgcggta tcgccggcct tgggcttgcc gatctccaag
     6721 aacagcgtcg cgcgcaggcc cttcaccagc tgcgcggtga cgatgtcgga ggcctcgtcg
     6781 gtgcggccga tccactgccg caaatgatcg agatcgggtt tgtcggtcat gacgatttgg
     6841 tccggacatg agaaggcttg
//
LOCUS       DATNOI010000107         7292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_528864,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000107 DATNOI010000000
VERSION     DATNOI010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528864"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VN968_03750"
     CDS             <1..469
                     /locus_tag="VN968_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXL28391.1"
                     /translation="SAAVENAASAADPGLSSFVGEEVAKSDRPELTSAKIIVSGGRAM
                     QSRENFAKYIEPLADKLGAGVGASRAAVDAGYAPNDWQVGQTGKVVAPELYIAIGISG
                     AIQHLAGMKDSKVIVAINKDEDAPIFQVADYGLVADLYQAVPELTEELGKLGK"
     gene            565..1446
                     /locus_tag="VN968_03755"
     CDS             565..1446
                     /locus_tag="VN968_03755"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HXL28392.1"
                     /translation="MAAMIKKVGVIGSGQMGNGIAHVAALAGFDVVLNDVSVDRLKSG
                     MATINGNLSRQVSKKIITEDARKQALDRIVLAESMDGLADCDIVIETAVEKEEIKRKI
                     FHDVCAVLKPEAIVASNTSSISITRLAASTDRPERFIGIHFMNPVPLMELVELIRGIA
                     TDDATFEASKEFVTRLGKYIAVSEDFPAFIVNRILLPMINEAIYTLYEGVGNVEAIDA
                     AMKLGAHHPMGPLELADFIGLDTCLSIMQVLHEGLADSKYRPCPLLVKYVEAGWLGRK
                     TQRGFYDYRGDKPIPTR"
     gene            1615..1809
                     /locus_tag="VN968_03760"
     CDS             1615..1809
                     /locus_tag="VN968_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28393.1"
                     /translation="MDMMAMVTSLLAAKSGGVQMQVAATIMKSNADAEKFAAQTLLGA
                     GASSLANVGAGVGGNLDISA"
     gene            1969..2304
                     /locus_tag="VN968_03765"
     CDS             1969..2304
                     /locus_tag="VN968_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HXL28394.1"
                     /translation="MKAHLRALPVSGTIAWVQYEWDNGKAAENLRKHGVDFADAIAAL
                     EDPNRLEEIDTRFEYSEERIQVIGMVRGDVLFVIVTLRGEDSCRIISARKATRHEQGR
                     YYAGHRETW"
     gene            2261..2635
                     /locus_tag="VN968_03770"
     CDS             2261..2635
                     /locus_tag="VN968_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXL28395.1"
                     /translation="MSKAGTTRVTVKPGDTLPRGDTDWARLDAMTDEEVVAAALSDPD
                     AQPLTPEQLSEMRRVSRVKVLRQRLGMTQMQFADAFHLPITTLRDWEQHRSTPDAPAR
                     ALLLAIERDPEVMRRLLAETAA"
     gene            complement(2735..3415)
                     /locus_tag="VN968_03775"
     CDS             complement(2735..3415)
                     /locus_tag="VN968_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXL28396.1"
                     /translation="MTNDMPDRPSPRPAATRRIPLAIGAVLIGAVIGFAGVYGIGGLK
                     RDAAGDSACRPAVDLARKLAPLAHGEVAALTMATVPLRLPDLAFEDTDGKPKKLSDWR
                     GRTVLVNLWATWCVPCRKEMPALESLQTRLGGADFEVVAINIDTRDPEKPKNFLKGAN
                     LTRLGHFSDQKAKVFQDLKAIGRAVGMPTSVLLDGQGCEIATIAGPAEWASEDAVKLI
                     TAAMKPPG"
     gene            3530..4927
                     /gene="argH"
                     /locus_tag="VN968_03780"
     CDS             3530..4927
                     /gene="argH"
                     /locus_tag="VN968_03780"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HXL28397.1"
                     /translation="MSNKMWGGRFTDRPDAIMEEINVSIDVDRHLYAQDIAASKAHAA
                     MLAAQGIISANDAKNIAKGLDTILSEIGKGSFDFKRALEDIHMNVESRLGELIGPAAG
                     RLHTARSRNDQVATDFRLYVRDTIDETDAALAAFQHALVERALEHAGTVMPGFTHLQT
                     AQPVTFGHHLLAYVEMAARDRGRFADARKRLNESPLGAAALAGTSFPIDRDATAKTLG
                     FDRPMANSLDAVSDRDFVLETLSAAAIASVHLSRFAEEIVIWTSPLVGLVRLSDKFTS
                     GSSIMPQKRNPDAAELVRAKTGRVIGALTALLIVMKGLPLAYQKDMQEDKQGAMEAFA
                     ALSLAIRAMTGMVADIMPDEARMKAAAGEGYATATDLADWLVRTLKMPFRDAHHVTGR
                     IVAMASKAGVPLHELPLKDMQAIEPKITAEVMAVLSVEASVKSRTSYGGTAPKNVLAQ
                     AKAWRKRLEKEQKLG"
     gene            5019..5297
                     /locus_tag="VN968_03785"
     CDS             5019..5297
                     /locus_tag="VN968_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HXL28398.1"
                     /translation="MNRTNCPTSSRWAVILLSAAVLALGGCGRKGGLDLPPNAASAQA
                     TAAEQADAEADRAASKPSVFNPTYGAESGPVAPKGRKKAFVLDPLLND"
     gene            5305..6570
                     /gene="lysA"
                     /locus_tag="VN968_03790"
     CDS             5305..6570
                     /gene="lysA"
                     /locus_tag="VN968_03790"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HXL28399.1"
                     /translation="MNHFDYRSGVLHAEAVNLSELADAVGTPFYCYSTATLERHYRVF
                     SDAFADEKALVCYAMKANSNQSVLRTLAKLGAGADVVSGGELKRALAAGIPRSKILFS
                     GIGKTEAELRAALLSDILCINVESEPELELLSRLASETGRIARISVRVNPDVDSGSHA
                     KISTGKSENKFGVPLDRARAVYARAAKLPGIEVTGVDMHIGSQITDLGPLEVAFNLLA
                     DFARVLRADGHTISHIDFGGGLGIPYHPDTTPPPEPAAYAAIVKRATRGLDCTLMFEP
                     GRMIVGNAGILVTRVIYVKHGEARNFVIVDAAMNDLIRPTLYEAYHDLLPVRLAEPGA
                     ASIVADVVGPVCESGDYLALDRSMPEPSSGDLLAVMTAGAYGAVQASSYNTRALVPEV
                     LVKDDQYAVVRPRVEVDTLIAMDKPAPWL"
     gene            complement(6638..>7292)
                     /locus_tag="VN968_03795"
     CDS             complement(6638..>7292)
                     /locus_tag="VN968_03795"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL28400.1"
                     /translation="RVRRFVDLSTVGSHMAVRIHDALAKRNIVQLDSPVSGGVGGAEK
                     GTLAVMVSGPKADFEAVKTALDVIGKVFFIGEKPGSAQTMKLANNLLSATAVVATSEA
                     VVMGVKSGLDASVMVDVINAGSGMNTASRDKFPRAILPRTFDFGFATGLMVKDVRLYL
                     EEAKALGLSMEVAEAVGRLWEVVIREMGAESDFTSAIKPIEQAAGVVVGGKTAHAAK"
BASE COUNT         1359 a         2267 c         2315 g         1351 t
ORIGIN      
        1 cagcgcagcg gtggaaaatg ccgcgtcggc ggccgatccc ggtctgtcca gttttgtcgg
       61 cgaggaagtt gccaagagcg atcgtcccga gctgacatcg gcgaagatca tcgtctcggg
      121 cggacgcgcc atgcagagcc gcgagaattt tgccaaatat atcgagccgc ttgccgacaa
      181 gctcggcgcc ggcgtcggcg cctcgcgcgc cgcggtcgat gccggatatg cgccgaacga
      241 ctggcaggtt ggccagaccg gcaaggtggt ggcgccggaa ctttatatcg ctatcggcat
      301 ttcaggcgcc attcagcatc ttgccggtat gaaggattcc aaggttatcg tcgcgatcaa
      361 caaggatgag gatgcgccga ttttccaggt cgccgattac ggcctggtcg cggacctcta
      421 tcaggcggtt cccgaattga ccgaagagtt gggcaagctc ggaaagtaac gatgatccaa
      481 acaatcggat gagcgaactt cggccggtgt tatacaaggg aatgggcaga cgcggatcgc
      541 gcgtcgctcc ggtgagatga caagatggcg gctatgatca agaaggtcgg cgtgatcggc
      601 tcgggccaga tgggcaatgg catcgcgcat gtggcggcgc tggccgggtt cgacgtggtg
      661 cttaacgatg tgtccgtcga tcgcctgaag tcggggatgg caaccatcaa cggcaatctg
      721 tcgcggcagg tctccaagaa aatcatcacc gaggatgccc gcaaacaggc gctggaccgg
      781 attgtattgg ccgagagcat ggatgggctc gccgattgcg atatcgtgat cgagaccgcg
      841 gtcgaaaagg aagagatcaa gcgcaagatt tttcacgatg tctgcgcggt cctgaagccg
      901 gaggccatcg tcgcttccaa cacctcctcg atctcgatca cgcggctggc cgcatccacc
      961 gaccggcccg agcgctttat cggcattcac ttcatgaatc cggtaccgct gatggaactg
     1021 gtcgaactga tccgcggcat tgccaccgac gacgcgacct tcgaggcgtc gaaggaattc
     1081 gtcacccggc tcggcaagta catcgcggtc tcggaagatt ttccggcctt tatcgtcaac
     1141 cgcattctgc tgccgatgat caacgaggcg atctatacgc tgtatgaagg ggtcggaaac
     1201 gtcgaggcga tcgacgctgc gatgaagctc ggcgcgcacc atccgatggg gccgctggaa
     1261 ctggccgatt tcatcggtct cgatacgtgc ctctcgatca tgcaggtcct gcatgagggt
     1321 ctggcggact ccaagtaccg gccgtgcccg ctgctggtga aatatgtcga ggccggctgg
     1381 ctcggccgca agacccagcg cggcttctac gactatcgcg gcgacaagcc gatcccgacg
     1441 cgctgacacg cctctggagt cggagtgcag ctaatgcgag gccatcagct cggccgcacg
     1501 atccgcggta ggctctgatg attctcgcgc cgccggttcc catcccctga cgcccaatct
     1561 ccagcattcg ttaacccttg ccgggttagg gtgccgcggt tggagtgttg ccttatggat
     1621 atgatggcca tggtcaccag tcttctcgcc gccaaatcgg gtggcgtgca gatgcaggtc
     1681 gccgccacga tcatgaagtc gaatgcggac gcggagaaat tcgcggccca gaccctgctg
     1741 ggcgcgggcg cgtcgtcgct ggccaatgtc ggcgcaggcg tcggcggcaa tctcgatatt
     1801 tcagcttaaa cccccttctt ccgtcattcc gcggcgcgcg ctggcgcgaa cctcagatgt
     1861 gcaattgcgc atctgaggtt cgtttgcttc actcgcgcgc cacttcgcca gagcacgctg
     1921 cggtggccga ctggttccgt ttccgcacaa tccacgccag agcacctcgt gaaggcgcac
     1981 ttgcgggcac taccggtatc cggtactatc gcctgggtac aatatgaatg ggacaatggc
     2041 aaagccgccg agaaccttcg taagcatggc gtggattttg cggatgcgat tgctgccctc
     2101 gaagatccga accgtttaga agaaatcgac acgcggttcg agtacagcga ggagcgaatt
     2161 caagtcatcg gaatggttcg cggcgacgtc ctattcgtga tcgttacgct gcgtggcgaa
     2221 gatagttgca gaatcatatc ggcccgaaag gccacacgac atgagcaagg ccggtactac
     2281 gcgggtcacc gtgaaacctg gtgatacatt gcctcggggc gacaccgatt gggcacggct
     2341 cgatgccatg accgatgagg aggtcgtggc cgctgcgcta tccgaccccg acgcccagcc
     2401 gctcactccg gaacaactgt ctgagatgcg ccgggtgtcg cgcgtaaagg tgctgcggca
     2461 acgcctcggc atgacgcaga tgcaatttgc tgacgcattt cacctgccga tcaccacgct
     2521 gcgcgattgg gagcaacatc gaagcacgcc cgatgcaccg gcgcgggcgc tgttgctggc
     2581 aatcgaacgt gacccggaag tcatgcggcg cttgctggcg gaaacagcag cgtagtggtt
     2641 cggattccgt cgcgtctcaa cggatcacat catagcggca cggtggagca atattgccca
     2701 tcttcatcga ccgtggctcc ccggaatgac gaggctaacc gggaggtttc atcgccgccg
     2761 taatcagctt gacggcgtcc tcgctggccc attccgcggg tccggcgatg gtggcgatct
     2821 cgcagccttg accatcgagc agcaccgagg tcggcatccc cactgcccgt ccgatagctt
     2881 taagatcttg aaaaaccttg gctttctgat cgctgaaatg gccaagccgg gtcagattgg
     2941 cgcccttgag gaagttcttc ggcttttcgg gatcgcgggt gtcgatgttg atggcgacca
     3001 cctcgaaatc ggctccgccc agcctggttt gcaggctctc cagcgcgggc atctccttgc
     3061 ggcagggcac gcaccaggtg gcccacaggt tcaccagcac ggttcgcccg cgccagtcgg
     3121 agagcttttt gggcttgccg tcggtgtcct cgaaagcgag gtccggcagc cgcagcggga
     3181 cggtcgccat cgtcagcgcg gcaacctcgc catgcgccag cggcgcgagc ttgcgggcaa
     3241 ggtcgaccgc cgggcggcag gccgaatcgc cggctgcatc gcgcttcagc ccgccgatcc
     3301 catatacccc ggcaaatcca atcaccgccc cgatcagcac cgccccgatc gcgaggggta
     3361 tccggcgcgt ggcagccggg cgaggcgagg ggcggtcggg catatcgttt gtcatcctgt
     3421 atcagatacg gctatgcaga gcctgtatta cggtcggttg agccactgat ggcccagcgc
     3481 gttcggttcg caggcgtgac gatgaatgac aggtcatgag taacaagtca tgagcaacaa
     3541 gatgtggggc ggccggttca ccgatcgccc cgatgcgatc atggaggaaa tcaacgtctc
     3601 catcgacgtc gatcgtcacc tctatgccca ggacatcgcc gcatccaagg cgcacgctgc
     3661 gatgctcgcc gcgcaaggca ttatttccgc gaacgatgcg aaaaatatcg ccaagggtct
     3721 agacacgatt ttgtcagaga tcggcaaggg atccttcgac ttcaagcgcg cgctcgagga
     3781 cattcacatg aatgtcgaga gcaggcttgg cgagttgatc gggcccgccg cggggcggct
     3841 gcacaccgcg cgttcgcgca acgatcaggt cgcaaccgat ttccggctgt atgtccgcga
     3901 taccatcgac gagaccgatg cggcgttggc cgcttttcag catgcgctgg tggagcgggc
     3961 gctggaacat gccggcacgg tgatgccggg cttcacgcat ctgcagaccg cgcagccggt
     4021 gacgttcggg caccatctgc tggcctatgt cgagatggcg gcgcgcgacc gcggccgctt
     4081 cgccgatgcg cgcaaacgtc tgaatgaatc cccgctcggt gccgcggcgc tggccggcac
     4141 atcgttcccg atcgatcgcg atgcgaccgc caagacgctc ggcttcgacc ggccgatggc
     4201 caattcgctc gatgcggttt cggatcgcga cttcgtgctc gagacgttgt cggcagcggc
     4261 gatcgcgtcg gtgcatctgt cgcgttttgc cgaggaaatc gtgatctgga cttcaccgct
     4321 ggtcgggctg gtgcgactga gcgacaaatt cacgtcgggc tcttcgatca tgccgcagaa
     4381 gcgcaacccc gatgccgccg aactggtccg cgccaagacc ggccgggtca tcggcgcgct
     4441 caccgctttg ctgatcgtga tgaagggcct gccgctggcc tatcaaaagg acatgcagga
     4501 agacaagcag ggcgccatgg aggcgttcgc cgcgctctcg ctggcgattc gcgcgatgac
     4561 cgggatggtc gccgacatca tgccggacga ggcgcggatg aaggctgccg ccggcgaggg
     4621 ctacgccacc gccaccgatc tggcggactg gctggtgcga acgctgaaaa tgccgttccg
     4681 cgacgcccat cacgtcaccg gacgcatcgt cgccatggcg tcgaaagcgg gcgtgccgct
     4741 gcacgagctg cccttgaagg acatgcaggc gatcgagccg aagatcacgg ccgaggtcat
     4801 ggccgtgctt tcggtggaag catcggtgaa aagccgcacc agttatggcg ggacggcgcc
     4861 gaagaacgtc ctcgcccagg ccaaggcgtg gcggaagcga ctggaaaaag agcaaaaatt
     4921 gggctgagcc cgggaaactc gctgcaaatt catgatggtc tgggtctcgc cagagcaggg
     4981 caatctttgt atggtgccgc cgcattgggg attttgccgt gaatcgtacg aactgtccga
     5041 catcttcgcg atgggccgtc atcctgctca gcgcggccgt gctcgcgctc ggcggctgcg
     5101 gacgcaaggg tggactagac ctgccgccga acgcagcatc ggcgcaggca accgccgctg
     5161 aacaggccga tgccgaggct gatcgggccg cgagcaagcc aagcgtgttc aatccgacct
     5221 acggcgccga atcggggcca gtcgccccca agggtcgcaa gaaagcattc gttctcgacc
     5281 cgctcctgaa cgactgagtc tgccatgaac cacttcgact atcgcagcgg cgtacttcac
     5341 gccgaggcag tcaatctttc tgagctggcg gatgcggtcg gcacgccgtt ttattgctat
     5401 tcgaccgcga ccctggagcg tcactaccgg gtgttcagcg acgcctttgc cgacgagaag
     5461 gcgctggtgt gttacgccat gaaggccaac tccaatcaat cggtgctgcg cacgctggcg
     5521 aagctcggcg ccggagcgga tgtggtttcg ggcggcgagc tgaagcgcgc gctggcggcc
     5581 ggcattccgc ggagcaagat cttgttttca gggatcggca agaccgaagc cgaactgcgc
     5641 gcggcgctgc tttcggacat tctctgcatc aacgtcgaat ccgagccgga gctcgaactg
     5701 ctctcgcggc ttgccagcga gaccggccga atcgcaagga tctcggtccg ggtcaatccc
     5761 gatgtcgatt ccggttccca cgccaaaatc tccaccggca agtccgagaa caagttcggc
     5821 gtgccgctgg accgcgcccg cgcggtctac gcccgcgccg cgaagctgcc ggggatcgag
     5881 gtcaccgggg tcgacatgca tatcggcagc cagatcaccg atctcgggcc gctggaggtc
     5941 gcgttcaacc tgctcgcgga cttcgcccgg gtgttgcgcg ccgacggtca taccatttcg
     6001 catatcgatt tcggcggcgg gttgggcatc ccctatcacc cggatacgac gccgccgcca
     6061 gagccggccg cctatgccgc catcgtcaag cgggcgacgc gcggcctcga ttgcacgctg
     6121 atgttcgagc ccggacggat gatcgtcggc aacgccggca ttctcgtgac gcgcgtgatc
     6181 tatgtgaagc atggcgaagc caggaatttc gtgatcgtcg atgcggcgat gaacgacctg
     6241 atccgcccca ctttgtacga ggcctatcac gacctgctgc cggtgcggct ggccgagccg
     6301 ggcgcagcct cgatcgtcgc cgacgtggtc ggaccggttt gcgaaagcgg cgactatctc
     6361 gcgcttgacc ggagcatgcc ggagcccagc tccggcgatc ttctggctgt catgacggcc
     6421 ggcgcctacg gcgcggtaca ggcctcaagc tacaacaccc gtgcgctggt gccggaagtg
     6481 ctggtcaagg acgaccagta cgcggtggtg cgaccgcgcg tcgaggtcga tacgctgatt
     6541 gcgatggaca agccggcgcc gtggctgtga tggcctcgtg tcccggaggc gatgcgaagc
     6601 atcgtccgcc gcgctgcgca tccagggcac gtgtccttca cttggcggcg tgagcggtct
     6661 tccctcctac caccacgccc gccgcctgct cgatcggctt gattgccgag gtgaaatcgg
     6721 actccgcgcc catttcgcga atcaccactt cccacagccg cccgaccgct tcggcaactt
     6781 ccatcgatag cccgagcgcc ttggcctctt cgaggtaaag ccgcacgtcc ttgaccatca
     6841 gcccggtcgc aaaaccgaaa tcgaaggtgc gcggcaggat cgcgcgcgga aacttgtcgc
     6901 ggctcgcggt gttcatgccc gagcccgcat tgatcacgtc aaccatgacg ctggcgtcga
     6961 gtcccgattt gacgcccatc accaccgcct cggacgtggc gaccacggcg gtggccgaca
     7021 gcaggttgtt ggcaagcttc atggtttgcg ccgagccggg tttctcgccg atgaagaaca
     7081 ccttgccgat cacgtcgagc gctgttttca ccgcttcgaa atccgctttc gggcccgaca
     7141 ccatcacggc aagcgttccc ttttcggcgc cgccgacgcc accgctcacg ggactgtcga
     7201 gttgcacgat gttgcgcttg gccagggcat cgtgaatccg caccgccatg tgtgacccga
     7261 cggtcgagag atcgacgaag cgcctgacgc gc
//
LOCUS       DATNOI010000108         6257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1555665,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000108 DATNOI010000000
VERSION     DATNOI010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6257
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555665"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VN968_03800"
     CDS             complement(<1..458)
                     /locus_tag="VN968_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL28401.1"
                     /translation="MTDAALTTIRLAYERADELESPTRRAMRRLFQRKGAVVGLVVIA
                     TFIMLALFAPWIVPYDPIATSWTLVRKPPSALHWFGTDDLGRDILGRVIYGARASLMA
                     GAISVGIALGVGLPFGLLSGYRGGFIDALISRITDAMLACPFLILAIALAA"
     gene            complement(462..1403)
                     /locus_tag="VN968_03805"
     CDS             complement(462..1403)
                     /locus_tag="VN968_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL28402.1"
                     /translation="MLSFLAKRVIQLIPTLFFVSILIFSLQHLLPGDPALVMAGEERD
                     PNVIAQIRAQYRLDQPIPIQYFYWVKGVLSGDFGESLRIKVPVRELIAQKLPVTMQLA
                     SMAIVIAFLIGIPAGIVSAVKKGTLWDYGANLFALWGISTPNFWLGIMLIFLFSIELG
                     WLPASGYVPLSENWRASLASTIMPAFVLGNAIAAILMRHTRSAMLQVLESDYVRTARA
                     KGLSERSVILKHAMRNALTPVITLGALELGTLLSGAVLTEQIFSIPGFGKLIVDAVFN
                     RDYAVVQGVVLVTATIYITLNLIADIAYILVNPRLRA"
     gene            complement(1633..3144)
                     /locus_tag="VN968_03810"
     CDS             complement(1633..3144)
                     /locus_tag="VN968_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL28403.1"
                     /translation="MKILRLATLAAALLLSLGAGVEAQTTLRIGLAEDPDILDPSIGR
                     TYVGRIVFAAVCDKLFDIDEKLNIVPQLALSHETSADGKEMTIKLRPGVKFHDGESFD
                     AEAAKFSLERHLTLPTSFRKPELASLDHVDVVDPLTIKLVLKSPYSPLIAQLTDRAGM
                     MVSPKAAREAGDKFGLHPVCAGPYKFVERVQQDRMVFEKFADYWNKDNVLIDRVVFLP
                     IVDVTVRLANLKSGGLDLIERVLATDIKDVRADPKLVLSTAPELGYLGLTININNDKN
                     KGALSQSEKVRQALDLSIDREAINQVVFNGEFTPGNQWVSPQHPYYQKAFPVQPRDIA
                     KAKALLKESGVALPVSVDYMIPKGAENEAVAQVVQSMAAEAGFDLKIRVVEFATTFKQ
                     AQAGEFQVFQINWSGRIDPDGNAYVFLHTKAPQNDGGYSTPEADKALEDARLTSDPAQ
                     RKAIYEKLTKVVLNDEPIIYLYHRKLLIAHTTKLEGYRQMPDGLVRVIGLKLK"
     gene            complement(3162..4274)
                     /locus_tag="VN968_03815"
     CDS             complement(3162..4274)
                     /locus_tag="VN968_03815"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HXL28404.1"
                     /translation="MMALLEVKNLVKHFVADRSVFGRPTAFIKAVDGVSFSVDAGQTL
                     ALVGESGCGKSTVSRLVLRLIEPDSGQIRFEGRDLLALNANQLRAFRRDAQIVFQDPY
                     ASLNPRMTVGQILTEPLALHDLVPPSGRRGRVEELLRLVGLEPRFARRYPHEFSGGQR
                     QRIAIARALAVEPKLVICDEPVSALDVSIRSQILNLLRDLQDRLGLAYIFVSHDLAVV
                     KHIADRVAVMNLGVIVETADTQALFASPRHPYSRALLSAIPVPKPRAKRSRILLQGEM
                     PSALNPPAGCRFHTRCPHVIERCRVEPPELLADSAGHMTACHRTAQLPPPDAIVPADG
                     AFSPALERLIAAFSGPVEVPASSGVDMVEPMPSAGV"
     gene            complement(4271..5260)
                     /locus_tag="VN968_03820"
     CDS             complement(4271..5260)
                     /locus_tag="VN968_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28405.1"
                     /translation="MTAGPLIEIEGLRVTFHGDDGRTTHAVDRVDLAVANGATLGLVG
                     ESGCGKSVTSLAIMGLLSKHSAEVTGSIRFDGFDLLEVPDQTLRDLRGNRLAMIFQEP
                     MTSLNPSFTIGDQIIETILRHRGGSQRAARARAIELLRRVHIPSPERRIDEYPHKLSG
                     GMRQRVMIAMALACDPRLLIADEPTTALDVTLQAQILDLMRELKAASGAAIILITHDL
                     GVVAEVCDDVAVMYAGEIVERAAVDELFASPQHPYTVGLLGSIPRLDRRASHLATIEG
                     MVPNMANPPSGCRFAARCPFVSDICVAAPPPMVSLSPGHWSRCIKAPLERLVS"
     gene            complement(5257..5967)
                     /locus_tag="VN968_03825"
     CDS             complement(5257..5967)
                     /locus_tag="VN968_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HXL28406.1"
                     /translation="MTWSIIARDKPTGQFGIAVATRFFAVGARVPHIAAGIGGIATQA
                     LVNPYYGIDGLKLLREGRKPRDIVETLIAADAGRESRQLHIMDAGGLVASHTGRDCVD
                     WCGHVEGKGFSIAGNMLAGARVLDDTAQTYVANERRPFAQRLIAAMRAGEAAGGDKRG
                     KQSAALLIYGNEEWSDLDLRVDDHTDPLGELERLEGVSRERWVHFRNYLPTRKNPAGI
                     TDRETIDAGIEAAIAGKK"
     gene            complement(5974..>6257)
                     /locus_tag="VN968_03830"
     CDS             complement(5974..>6257)
                     /locus_tag="VN968_03830"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXL28407.1"
                     /translation="VHARMNLQEAIDAPAWHSEHFPISFWPRTARPGVLVVENRVPKA
                     TVDTLKSRGHIVETGPDWSEGRLTAASKVGRRRRAAANPRGMQGYAAGR"
BASE COUNT         1175 a         1943 c         2061 g         1078 t
ORIGIN      
        1 gccgccagcg cgatcgcaag gatcaggaac gggcaggcca gcatggcgtc ggtgatgcgg
       61 ctgatcaggg cgtcgatgaa gccaccgcga tagcccgaca acagtccaaa cggcaggccg
      121 accccaagcg cgatcccgac cgagatcgcg cccgccatca gcgaggcgcg cgcgccatag
      181 atcacgcgcc cgagaatatc ccgaccgagg tcgtcggtgc cgaaccagtg cagcgccgag
      241 ggcggcttgc gcaccagcgt ccagctggtc gcgatcggat catagggcac gatccagggc
      301 gcaaacagcg cgagcatgat gaaggtcgcg atcacgacga gcccgaccac cgcgcccttg
      361 cgctggaaca gccggcgcat tgcgcgccgc gtcgggcttt caagctcatc ggcgcgttca
      421 taggcaagcc ggatcgtggt gagcgcggcg tcggtcatgg cttacgccct cagccttgga
      481 ttgaccagga tataggcgat atcggcgatc aggttcagcg tgatgtagat ggtggcggtc
      541 accagcacca cgccttgcac cacggcatag tcgcgattga ataccgcgtc gacgatcagc
      601 ttgccaaaac ccggaatcga aaaaatctgc tcggtcagca ccgcgcccga cagcagcgtg
      661 cccagttcga gcgcgccgag cgtgatgacc ggcgtcaacg cgttgcgcat ggcgtgcttg
      721 agaatgaccg agcgttcaga aaggcccttg gcgcgggcgg tacggacata atcgctttcg
      781 agcacttgca gcatggcgct gcgggtgtgc cgcatcagga tcgcggcgat ggcattgccg
      841 agcacgaagg cgggcatgat ggtggacgcc aggctggcgc gccaattctc cgagagaggg
      901 acatagcccg atgccggcag ccagccgagc tcgatcgaga acaggaagat cagcatgatc
      961 ccgagccaga aattcggcgt cgagatgccc cacagcgcaa acagattggc gccgtagtcc
     1021 cacagcgtgc ccttcttcac cgccgacacg atgccggcgg gaatgccgat cagaaacgcg
     1081 atcacgatcg ccatcgacgc cagttgcatc gtcaccggca gtttttgcgc gatcagttcg
     1141 cgcaccggca ccttgatgcg gagcgactcg ccgaaatcgc cggacaatac gcccttgacc
     1201 caatagaaat attgaatcgg gatcggctga tcgagccggt attgcgcgcg gatctgcgcg
     1261 atgacattgg gatcgcgttc ctcgccggcc atcaccagcg ccggatcgcc cggcagcaaa
     1321 tgctgcagcg agaagatcag gatcgacacg aagaacagcg tcgggatcag ctggatgacc
     1381 cgcttggcga gaaagctcag catgaagcac gcacaatttg acgcgcggtg aggctaaatc
     1441 gggttgaaag tgcccgccac ggcgttccct ccccccttgc gggggaggga cagggagggg
     1501 ggtgacccaa cgggattctg tcctcgtggc accccccacc ccaaccctcc cccgcaaggg
     1561 gacccgcaag gggggaggga gtagaaagat gcgctgcatg gtgtccggga cacgagggca
     1621 tgtgccggct cctcacttca atttcaaccc gatcacgcgt accagcccgt ccggcatctg
     1681 cctgtagcct tcaagcttgg tggtatgcgc gatcaggagc ttgcggtggt agagatagat
     1741 gatgggttcg tcgttgagca cgaccttggt cagcttttca tagatcgcct tgcgctgcgc
     1801 tggatccgag gtgagacgcg catcctccag cgccttgtcg gcctcggggg tcgaatagcc
     1861 gccatcgttc tgcggcgcct tggtgtgaag aaagacatag gcattgccgt cgggatcgat
     1921 gcggccgctc cagttgatct ggaacacctg gaactcgccg gcctgggcct gcttgaaggt
     1981 cgtcgcgaat tcgacgacgc gtattttcag atcaaaaccc gcttcggccg ccatcgactg
     2041 aacgacctgg gccacggctt cgttctccgc gcccttgggt atcatgtaat cgacgctgac
     2101 cggcagcgcg acgccggatt ccttcaacaa cgccttggcc tttgcgatgt cgcgcggctg
     2161 aaccggaaag gccttttggt agtaaggatg ctgcgggctg acccactgat tgccgggcgt
     2221 gaattcgccg ttgaacacga cctggttgat ggcctcacga tcgatcgaca gatcgagcgc
     2281 ctggcgcacc ttttccgact ggctgagcgc acccttgttc ttgtcgttgt tgatattgat
     2341 ggtaaggccg agatagccga gttcgggcgc ggtcgacagc acgagcttcg gatcggcgcg
     2401 cacgtccttg atgtcggtcg cgagcacgcg ctcgatcaga tcgagcccgc cggatttcag
     2461 attggcgagc cgcaccgtga catcgacgat cggcagaaac accacgcggt cgatgaggac
     2521 attatccttg ttccagtaat cggcgaattt ctcgaacacc atgcgatcct gctgcacgcg
     2581 ttcgacgaat ttgtagggcc cggcgcaaac cggatgcagc ccgaacttgt cgccggcttc
     2641 cctggccgcc ttgggcgaga ccatcatccc ggcgcgatcg gtgagctgcg cgatcagcgg
     2701 cgagtaaggc gacttcaata ccagcttgat ggtcagggga tcgacgacat cgacatggtc
     2761 cagcgaggcg agttccggct ttcggaacga ggtcggcagc gtgaggtggc gttccagcga
     2821 gaatttcgcg gcctcggcgt cgaacgactc gccgtcgtgg aatttgacgc ccgggcgcag
     2881 cttgattgtc atttccttgc cgtcggccga agtctcgtgc gatagcgcga gctgcggcac
     2941 gatgttgagc ttctcgtcga tgtcgaacag cttgtcgcag accgccgcga atacgatgcg
     3001 gccgacatag gtgcgcccga tcgacgggtc caggatatcc gggtcctcgg caaggccgat
     3061 gcgcagggtg gtctgggctt caacgcccgc tccaagcgac agcagcaacg ccgccgcaag
     3121 ggtcgccaaa cgcaggattt tcatcagact ggtctccata ttcaaacgcc ggccgaaggc
     3181 atcggctcga ccatatcaac cccggaggaa gcaggtactt ccacgggccc gctgaaagcc
     3241 gcgatcaatc gctccagtgc cggcgaaaag gcgccatcgg cagggacgat ggcgtcaggc
     3301 ggcggcagtt gcgcggtgcg atgacacgcg gtcatgtgtc ctgcgctatc tgcgagcagt
     3361 tccggcggct caacgcggca gcgctcgatc acatgggggc agcgggtatg aaagcggcag
     3421 cccgccggcg gattgagcgc actcggcatc tcgccctgca gcagaatgcg gctgcgcttt
     3481 gcccgcggtt tgggcaccgg gatcgcggac agcagcgcgc ggctgtaggg gtgacgcggc
     3541 gaagcgaaca gcgcctgtgt atccgcggtc tcgacgatca cgccgagatt catcacggcg
     3601 acgcggtcgg cgatgtgctt gacgacggcg aggtcatgcg agacgaagat gtaggcgagg
     3661 ccaagccggt cctgcagatc gcgcagcaaa ttgaggatct gcgagcggat cgacacatcc
     3721 agagccgata ccggctcgtc gcagatgacc agttttggct ccaccgctag tgcgcgggca
     3781 atggcgatgc gctggcgctg gccgccggaa aattcgtggg ggtagcgccg cgcgaagcgc
     3841 ggctccagtc cgaccaggcg caacaattcc tcgacgcggc cgcgacggcc tgagggtggc
     3901 accagatcat gcagcgccag cggctcggtc aggatctggc cgacggtcat gcgcggattg
     3961 agcgacgcat agggatcctg gaagacgatc tgggcatcgc ggcggaatgc gcgtaattgg
     4021 ttggcgttca acgcgagcag gtcgcggccc tcgaagcgga tctggcctga atccggttcg
     4081 atcagccgca gcaccagccg gctcacggtg gatttgccgc aaccggattc gccgaccagc
     4141 gccaacgtct ggccggcatc gacgctgaag ctgaccccat cgaccgcctt gatgaacgcc
     4201 gtcggccgcc cgaacaccga ccggtcggcg acgaaatgtt tgaccaggtt tttgacttcg
     4261 agcagggcca tcatgacacc agccgctcca gcggcgcctt gatgcagcgc gaccagtgac
     4321 cgggactcaa actgaccatc gggggtggtg ctgcgacgca gatgtcgctg acaaaaggac
     4381 aacgcgcggc gaagcggcag ccggacggcg gatttgccat gttcggcacc atgccctcga
     4441 tggtggccag atgcgatgcg cggcggtcga gccgcgggat cgagccgagc aggccgacgg
     4501 tgtagggatg ctgcggagat gcgaacaatt catcgaccgc ggcacgttcg acaatctcgc
     4561 cggcatacat caccgcgacg tcgtcgcaga cctcggcgac cacgccgaga tcgtgggtga
     4621 tcaggatgat cgcagccccg ctcgccgcct tcaattcgcg catcagatcg aggatttgcg
     4681 cctgcaaggt cacgtcgagt gccgtggtcg gctcgtccgc gatcagcagc cggggatcac
     4741 aggccagcgc catcgcgatc atcacgcgct ggcgcatgcc gccggacagc ttgtgcggat
     4801 attcgtcgat gcgccgctcc ggcgaaggga tgtggacgcg gcgcagcagt tcgatcgcgc
     4861 gtgcgcgggc ggcgcgctgc gagccgccgc gatggcgcag gatggtctcg atgatctgat
     4921 cgccgatggt aaaactcgga ttgagcgagg tcatcggctc ctggaagatc atcgcgaggc
     4981 ggttgccgcg caggtcacgc agggtctggt cgggcacctc cagcagatca aaaccgtcga
     5041 accggatcga gccggtgact tcggccgaat gctttgacag caggcccatg atggccagcg
     5101 aggtgacgct ttttccgcag ccggattcgc cgacaaggcc gagcgtcgcg ccattggcga
     5161 cggcaaggtc gacgcggtcg accgcgtggg tggtgcggcc gtcgtcgccg tggaaggtga
     5221 cccgcaagcc ctcgatctcg atcagcgggc ctgccgtcat ttcttgcctg caatcgccgc
     5281 ttcaatgccg gcgtcgatgg tctcgcgatc ggtaattccg gccggattct tgcgggtcgg
     5341 cagatagttt cggaaatgca cccagcgctc gcggctgacc ccttcgaggc gttcgagttc
     5401 gccaagcgga tcggtgtgat catcgacgcg cagatcgaga tcagaccatt cttcgttgcc
     5461 gtagatcaga agtgccgccg actgcttgcc gcgcttgtcg ccgccggcgg cttcgccggc
     5521 gcgcatcgcc gcgatcaggc gctgggcgaa gggccgtctt tcgttggcga cgtaggtctg
     5581 cgcggtatcg tccagcacgc gggcgcccgc gagcatgttg ccggcgatcg aaaaaccctt
     5641 gccttcgacg tgcccgcacc agtcgacgca gtcgcgcccg gtatgcgacg cgacaagtcc
     5701 gccggcgtcc atgatgtgca actgccggct ttcgcggccg gcgtccgccg caatcagggt
     5761 ctcgacgatg tcgcgtggct tacgtccctc gcgcaacaac ttcaaaccat cgatgccgta
     5821 atagggattg accagcgcct gcgtcgcgat accgccgata ccggcggcga tgtgcggcac
     5881 ccgcgcgccc accgcgaaaa aacgtgtcgc aaccgcgata ccgaattggc cggtcggctt
     5941 gtcgcgggcg atgattgacc aggtcatctc ggcttatcgt ccggccgcat agccctgcat
     6001 gccgcgggga ttggcggcag cacgtcggcg gcgaccgacc ttcgaggccg cggtgaggcg
     6061 gccctccgac cagtcgggac cggtctcgac gatatggccg cggctcttca aggtgtcgac
     6121 ggtcgccttc ggcacgcggt tctcgaccac gagcacgcct ggacgggcgg tgcgcggcca
     6181 gaacgagatc gggaaatgct ccgaatgcca tgccggcgcg tcgatcgctt cctgcaggtt
     6241 catccgggcg tgcacgt
//
LOCUS       DATNOI010000109         9501 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1936048,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000109 DATNOI010000000
VERSION     DATNOI010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1936048"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /gene="bfr"
                     /locus_tag="VN968_03835"
     CDS             complement(<1..436)
                     /gene="bfr"
                     /locus_tag="VN968_03835"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HXL28408.1"
                     /translation="MQGDAKVIEYLNKGLRSELTAINQYWLHYRVLNNWGLLEMAKTW
                     RKESIEEMEHADKFTDRILFLDGFPNMQVLDPLRIGQNVKEIIECDLASEVAARALYQ
                     EAATYCHGVKDYVSRDLFESLMKDEEHHIDFLETQLDLIGRIG"
     gene            complement(572..856)
                     /locus_tag="VN968_03840"
     CDS             complement(572..856)
                     /locus_tag="VN968_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL28409.1"
                     /translation="MIVCSCNVLSDDDVRNAVNASESITRNAKQIYGCLGCSAECGRC
                     ARTIKTIIDDALGACAKACCSGCPHSRQAVADLTDADSRAHGELSLEAAS"
     gene            complement(1037..1432)
                     /locus_tag="VN968_03845"
     CDS             complement(1037..1432)
                     /locus_tag="VN968_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28410.1"
                     /translation="MNGPAIFRDQYGLSHGIFAPHNAIFRREKTMTSAVAAFAMAVGA
                     TSLICYLLMTRLPNRRTNRGSSGNGFGADGLDFGGSGGWSLSHWFGGDHSALDGSGNP
                     IDFSGGDSGGGGDSGGGGDGGGDGGGGGD"
     gene            1513..1836
                     /locus_tag="VN968_03850"
     CDS             1513..1836
                     /locus_tag="VN968_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28411.1"
                     /translation="MRKTLMALAAVATLAVSAVAAPAPAHAQRGFGPGLAAGLIGGAI
                     VGGALASPGYYGPGYGYYGPRYGYYGGPAYVADGYDYYGSCVWQRQRFWDGYGWRIRR
                     VRVCG"
     gene            2049..2315
                     /locus_tag="VN968_03855"
     CDS             2049..2315
                     /locus_tag="VN968_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28412.1"
                     /translation="MKKTFAAFVAVATIAGSLAATPASAQRGVAAGVAAGLIGGAIVG
                     GAIASSRPAYGGPVYVEEAPPCRWVRERFWDGYGWRFRRVEVCN"
     gene            complement(2491..3723)
                     /locus_tag="VN968_03860"
     CDS             complement(2491..3723)
                     /locus_tag="VN968_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HXL28413.1"
                     /translation="MGVRRAVELGVLVLTLAFHSPARAADAGFAQFIASLWPEAQAAG
                     VSRATFDAETRGLEPDYKLPDLILPGRPATGAPSQAEFVQVPADYIKEASIARLAEEG
                     QRLMAKYRDALSAIEARFGVPATVVLAIWGRETDYGRYTLPYDAVRVLATQAYVGRRK
                     DQYRTEFILALKIIGDGEVARKDMRSSWAGATGLTQFLPSEFYKHGVDFDGDGRRDIW
                     NSVPDALASAAQQLVNKGWQPGLRWAYEVRAPAGVDCTLGVPEVTKPIGEWLRDGFAP
                     VRGQKLSAAEQAQPASLLQPEGIYGPAFLTTKNYFVIKEYNFSDLYVLFVGHLSDRMV
                     TPQPFATPWSASTQLRTADVEAMQRHLTRIGLYADKLDGKAGMKTRSALGAYQKSAGL
                     KVDCWPSEAVLRSIGAAR"
     gene            complement(3738..3938)
                     /locus_tag="VN968_03865"
     CDS             complement(3738..3938)
                     /locus_tag="VN968_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28414.1"
                     /translation="MAETDNIVLEPLRHIRGAIDSVRDDIRKIKQRVGNLENQYANMS
                     NRLDRMDLRIERIERRLDLTDA"
     gene            complement(4036..4806)
                     /gene="tam"
                     /locus_tag="VN968_03870"
                     /pseudo
     CDS             complement(4036..4806)
                     /gene="tam"
                     /locus_tag="VN968_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315687.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
     gene            complement(4822..6471)
                     /gene="ettA"
                     /locus_tag="VN968_03875"
     CDS             complement(4822..6471)
                     /gene="ettA"
                     /locus_tag="VN968_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HXL28415.1"
                     /translation="MARQFVYFMQGLSKTYPTRKVLDNVHLSFYPDAKIGVLGVNGSG
                     KSTLLRIMAGLDKEYNGEAWVAEGARVGYLEQEPQLDPARTVRQNVMLGVGKQKAILD
                     RYNDLAMNYSEETADEMTKLQDEIEAAGLWDLDSKVDQAMDALRCPPDESDITKLSGG
                     ERRRVALCKLLLDQPDLLLLDEPTNHLDAESVSWLEGHLRNYPGAILIVTHDRYFLDN
                     VTSWILELDRGKGIPYEGNYTAWLSQKQKRLAQEGREDAAHQRTLEREKEWIAASPKA
                     RQAKSKARYQRYEELLKQASEKQTQTAQITIPVAERLGQNVVDFEGLTKGFGDRLLID
                     DLTFKLPPGGIIGVIGPNGAGKTTLFRMITKQETPDKGTITVGESVHLGYVDQSRDSL
                     DGKKSVWEEISGGNDLILLGKREVNSRGYCSSFNFKGADQQKKVGALSGGERNRVHLA
                     KMLKSGANVLLLDEPTNDLDVDTLRALEEALEDFAGCAVIISHDRWFLDRIATHILAF
                     EDDSHVEWFEGNFQDYEKDKMRRLGQDSIIPHRMKYKKLTR"
     gene            6724..6927
                     /locus_tag="VN968_03880"
     CDS             6724..6927
                     /locus_tag="VN968_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28416.1"
                     /translation="MTTITSETLSSTTGSRDGSSLFAEIRAFWRQFLARAFDPYRPEL
                     HYMRGPGPAWHAKQQRSPRAHSA"
     gene            7095..8054
                     /locus_tag="VN968_03885"
     CDS             7095..8054
                     /locus_tag="VN968_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HXL28417.1"
                     /translation="MTRLRCAILDDYLNLSLTAADWSKIGDRVDVTVFNQPFASPEAA
                     AGALKDFEIICAMRERTPFPRTLFAALPKLKLLITSGMRNAALDLEAAKDHQVVLCGT
                     QWARDPTAPLTMGLILELTRNIGRENARMHAGEPLQKFVGIEIEGRTLGVVGLGKLGA
                     KVSALAKAFGMNVIAWSPNLTPERCKEVGVGYATKEELFATADIITVHVVLSQRSRGL
                     VGAADLARMKPTSYLVNTARGPIVDEAALLETLQRKKIAGAAVDVFSVEPLPVDHPFR
                     KLDNIVLTPHLGYVTEEGFRNHYVQMVEGIDAWIKGEPLRRLV"
     gene            complement(8106..9269)
                     /locus_tag="VN968_03890"
     CDS             complement(8106..9269)
                     /locus_tag="VN968_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXL28418.1"
                     /translation="MVPAHFHDRTLSIMAMRFGDGSAGRGITRFWTSWVPVPQDFAFA
                     WLNSHFLKLPAAQLQRIQELNMRVFVAGATGAVGRQLVPALVASGHSVVGLTRTAAKR
                     ETIKQMGAEPVVADGLDGGAVGAAVTSTKPDIVIDEMTDLSDVTNFRHFDRSFANTNR
                     LRTRGTDLLLAAAREAGVKRFIAQSFCGWAFARAGGPVKSEADALDPDPPEELRRTLE
                     AIQYLERTVTDSTNPEGIVLRYGSFYGPDTGMLSRPMVEQLRHRRVPLIGDGSGWWSF
                     VHVDDAAAATVSAIERGKAGNIYNIVDDHPAQVREWLPALAEMLGAKPPFHVPAWIAR
                     LVAGEHMVAMMTQVRAGSNAKAKTDLGWWPAHPSWREGFAEIAKPSFERRSAA"
BASE COUNT         1786 a         2971 c         2882 g         1862 t
ORIGIN      
        1 gcccgatccg cccgatcaga tcaagctggg tttcgaggaa atcgatgtga tgctcctcgt
       61 ccttcatcag gctttcgaac agatcgcgtg agacgtagtc cttgacgccg tggcaatagg
      121 tcgcggcttc ctggtaaagc gcgcgggcgg caacttcgga agcaagatcg cattcgatga
      181 tttccttgac attctgcccg atccgcaggg gatcgagcac ctgcatgttg ggaaagccgt
      241 cgaggaacag aattcggtcg gtgaatttgt ccgcgtgctc catctcctcg atggattcct
      301 tgcgccaggt cttggccatc tccagcaggc cccagttgtt caacacccga taatgcagcc
      361 agtactggtt gatggcggtg agttcgctgc gcaggccctt attgaggtat tcgatgactt
      421 ttgcgtcgcc ctgcatggtg cactctcgct gccggggccg atccgggcgg ccctcatagg
      481 gtaagttaga acgctactaa gtcacattat gaacgagagc aaccctcccg ccgaaacggc
      541 cggagaggga caagcgcaat atttgaaggt ttcaggaagc agcttcgagg gagagctcgc
      601 cgtgcgcgcg cgagtccgca tccgtcaagt ccgcgaccgc ctggcggctg tgcgggcatc
      661 cggagcagca ggccttggcg cacgcaccga gagcgtcgtc aatgatggtc ttgatggtgc
      721 gcgcgcatcg tccacattcg gcgctgcaac cgaggcagcc gtagatctgc ttggcattgc
      781 gcgtaatgga ttccgatgcg ttgacggcgt tgcggacatc gtcgtcgctg aggacgttac
      841 aggaacaaac aatcatgaaa acgcgcaggt ccttcggtga tgcgggccac aggtattcaa
      901 gactgcggcg ggatgcaaaa ggaaaatccg tcttttcaag tgattccaaa gtgatgcgtg
      961 acacagacta gaatgaatct aaaaaaaccg cgcttgcggc ggaccaaaaa gccactgctt
     1021 ttctcgcccg gcagcgtcaa tcgccgccgc cacccccgtc gccgccgcca tcaccgcctc
     1081 ctccactatc gccgccgcct ccgctatcgc cgccgctgaa gtcgatcgga ttgcccgaac
     1141 cgtccagagc tgaatgatcg ccaccgaacc aatgagaaag gctccagccg ccgctgccgc
     1201 caaaatcaag gccatccgct ccgaagccat tgccagacga tcctcgattg gtgcggcggt
     1261 tcggcaatcg ggtcatcagc aggtagcaaa tcagtgatgt cgcgccgacc gccattgcga
     1321 atgccgccac agcgctcgtc atcgttttct ctcgcctgaa aatagcattg tgcggtgcaa
     1381 aaataccgtg ggacagccca tattggtcgc gaaatatggc gggtccgttc attgatcatt
     1441 caagcgaaat gtggaacttt cgccgccgga acgcttcgtc agctgtcctt gctccatgag
     1501 aaaggtgaag ccatgaggaa aacattgatg gcgctcgctg ccgtcgccac cctggccgtt
     1561 tcggccgtgg cggcacccgc gccggcgcac gcccaacgtg gttttggtcc aggtctcgct
     1621 gccggcttga tcggcggcgc catcgtcggc ggcgcgcttg ccagtcctgg ctattacggc
     1681 cccggctatg gctattacgg accacgctat ggctactacg gcggcccggc ctatgtcgct
     1741 gacggctacg actattacgg cagctgcgtc tggcagcggc agcgcttctg ggatgggtat
     1801 ggctggcgga ttcgccgtgt cagggtgtgc ggctgatccc gttcaccttc attacaaagc
     1861 gtccggttgt tccgcagaca acaggacggc ctgaaaaaac ctttttttcg ggccactagc
     1921 cgtaacgacg tttactttcg attgactgct gtgcgctttt tagcgaaacg gccggtttcg
     1981 aacacagcgt gtgagtcatc ttaaacccgg cgctgacgca aagcgccatc ttctggagag
     2041 ccaaagaaat gaagaagaca tttgctgcct tcgtcgcggt cgcaacgatc gctgggtcgc
     2101 tcgcagcgac acccgcaagc gcacagcgtg gcgtcgccgc aggcgtggcg gccggactga
     2161 tcggcggcgc catcgtcggc ggggccatcg ccagcagccg tccagcctat ggcggtccgg
     2221 tttacgtcga agaggctccc ccttgccgct gggttcgcga gcgtttctgg gatggttacg
     2281 gctggcgctt ccgccgcgtc gaggtttgca actgatctga cccgatcggc gcggaattca
     2341 ccgcgccttt atttgaaaac ccggccgaag tttcggccgg gtttttgttt gtgtgcgcat
     2401 gctttaggat cgttatgctt taacctctcc catcggccgc ttatgctctc ccaatgggag
     2461 aggtgggaaa ccttctacgc ttcggcaggc ctagcgcgcc gcgccgatcg aacgcagcac
     2521 cgcttcgctc ggccagcagt ccactttgag cccagccgat ttctgataag ccccgagcgc
     2581 tgagcgcgtc ttcattcctg ccttgccgtc cagtttgtcg gcatagagac cgatgcgcgt
     2641 gaggtgacgc tgcatggctt cgacgtcggc agtgcgcagc tgcgtcgacg ccgaccacgg
     2701 cgtcgcgaac ggctgcgggg tcaccatgcg gtcgctgagg tggccgacga acagcacata
     2761 caggtccgaa aaattatatt ccttgatgac gaagtaattc ttcgtggtca gaaacgccgg
     2821 gccatagata ccttcgggct gcagcaatga ggccggctgc gcctgctcgg cggcactgag
     2881 tttctggccg cgcaccggtg caaaaccatc gcgcaaccat tcgccgatcg gttttgtcac
     2941 ctctggcacg cccaacgtac aatcgacacc agccggcgcg cgcacctcat aggcccagcg
     3001 caggcccggc tgccagccct tgttgacgag ttgctgcgcg gcggaggcga gcgcatccgg
     3061 caccgaattc cagatgtcgc ggcggccgtc accatcgaaa tcgacgccgt gcttgtagaa
     3121 ttccgatggg aggaattgcg tgagaccggt ggcgccggcc caggacgagc gcatatcctt
     3181 gcgcgccact tcgccatcgc cgatgatctt cagcgccaga atgaactcgg tgcggtattg
     3241 atccttgcgg cgaccgacat aggcttgcgt ggccagcacc cgcaccgcat cgtagggcag
     3301 cgtgtagcgg ccatagtcgg tttcgcggcc ccagatcgcc agtaccacgg tggcgggcac
     3361 gccgaagcgc gcttcgatcg cgctcaaggc gtcgcgatac ttcgccatca gccgttgccc
     3421 ctcctccgcc agccgcgcaa tgctggcttc cttgatgtaa tccgccggca cctgcacgaa
     3481 ctcggcctgc gatggcgcgc cggtcgcggg acgtcccggc aatatcagat cgggcaattt
     3541 gtaatccggc tcaagaccgc gggtctcggc atcaaaagtc gcgcgcgaca ccccggcggc
     3601 ttgcgcttcc ggccacagcg aggcgatgaa ctgcgcaaaa ccggcgtcgg cagcacgggc
     3661 gggagagtga aaggccagcg tcagaaccaa gacgccaagc tccaccgcgc ggcggacgcc
     3721 caaccggaat aaccagctca cgcgtcggtc aaatcgagac gccgctcgat acgctcgata
     3781 cggaggtcca tccggtcaag ccggttcgac atattggcat attggttctc aaggttgccg
     3841 acgcgctgct ttatcttgcg aatatcgtcg cgcacgctat cgatcgcgcc acggatatga
     3901 cgcagaggtt caagaacaat gttgtccgtc tcggccatgg tcgcagacta tcatagcggc
     3961 agatcattgt gaacaatgac cgccggcagc cgcgaacgaa gaatccaatc agcgctgaat
     4021 tccggggaaa tgcaactacc tcaccgccac gataaaaatc cgcggaaacc gcagcagcac
     4081 cttgccgtcg gcctgcggca gatagctggc ggcgatgcgc gcggtgtatt cggcgagata
     4141 ggccttgcgt tcgggcggat ccaggggatc gacgaacggc cgcaggccgc ttcccttgac
     4201 ccactccacg atcgcggcgg catcgtcgag cacgtggtta tagatcgtgt gccagatttc
     4261 cagccggctg cagagcgggc ctagcgcatc gtaatagacg cccggcttgg gaagggcgtc
     4321 gcggagccgt gcggtctcgg ccagcgtcgt gcgccacggc tccagcagcg caacctcgcg
     4381 catcaggaca tgggcgggct catcgagatt gtcgggcatt tgcaccgcaa ggacgccgcc
     4441 cggcgtcaac gcgccgagca gccgctgcag ttgcctgaga tggccgggca cccactggaa
     4501 gatcgcattg gcaaacagca cgtcggtgtt ttccggcggc actcagtgcg cgacgttggc
     4561 ctcgacaaac gtccgtcccg gcagccgttc gcgcgcctgc cgcagcatgt cggccgaagt
     4621 atcgatgccg atcgcctctg cctgcggcca gcgcctgacc agaagctcgg ttgaatttcc
     4681 cggtccgcat ccgatatcga cgaccctgcg cacctcggca agcgggattt gcgccaagag
     4741 gtcgcgagac ggtcgggtcc gctcatcctc gaacttaaga tattgctcag cgctccagtc
     4801 cgccatctcc aggattccgg cttaccgcgt cagcttcttg tacttcatgc gatgcgggat
     4861 gatggaatcc tgtcccagcc tgcgcatctt gtctttttca taatcctgga aattgccctc
     4921 gaaccattcg acatggctgt cgtcctcgaa ggccaggata tgcgtggcga tgcggtcgag
     4981 aaaccagcgg tcatggctga tgatcaccgc gcagccggcg aaatcctcca gcgcctcttc
     5041 cagcgcgcgc agcgtgtcga cgtcgagatc gttggtcggc tcgtcgagca gcagcacgtt
     5101 ggcgccggat ttgagcatct tggccagatg cacgcggttg cgctcgccgc ccgacagcgc
     5161 gccgaccttc ttctgctgat cggcgccctt gaaattgaac gacgagcaat agccgcgcga
     5221 attgacctcg cgcttgccca gaagaatgag atcgttgccg ccggagattt cctcccacac
     5281 gctcttcttg ccgtcgagac tgtcgcgcga ctggtcgaca tagccgagat gcacgctctc
     5341 gccgaccgtg atggtgccct tgtccggcgt ctcctgcttg gtgatcatcc ggaacagcgt
     5401 ggtcttgccg gcgccgttgg ggccgatcac gccgatgatg ccgccgggcg gcaatttgaa
     5461 ggtgaggtca tcgatcagaa gccggtcgcc aaagcctttg gtcaggcctt cgaaatcgac
     5521 cacgttctgg ccgaggcgct cggccaccgg aatggtgatc tgcgcggtct gggtctgctt
     5581 ctcgctggcc tgcttcaaga gttcttcgta gcgctggtag cgtgccttgg atttggcctg
     5641 gcgtgctttc ggcgaggccg cgatccactc cttctcgcgc tccagggtgc gctggtgggc
     5701 ggcgtcctcg cggccttcct gcgccagccg tttctgcttc tgcgacagcc aggcggtgta
     5761 gttgccctcg tagggaatgc ccttgccgcg gtcgagttcg agaatccagc tggtgacgtt
     5821 gtcgaggaaa tagcggtcat gggtgacgat caggatcgcg ccgggatagt tgcgcaaatg
     5881 gccttccagc catgacaccg actcggcgtc gagatggttg gtcggctcgt cgagcagcag
     5941 gaggtcgggc tgatcgagca gcagcttgca cagcgcgacg cggcggcgct cgccgccgga
     6001 aagtttcgtg atatcggatt cgtcgggcgg gcagcgcagc gcgtccatcg cctgatcgac
     6061 cttgctgtcg agatcccata ggccggcggc ctcgatctcg tcctgcagct tggtcatctc
     6121 gtcggcggtt tcctcggaat aattcattgc gaggtcgttg tagcggtcga ggatcgcttt
     6181 ctgcttgccg acgccaagca tcacgttctg gcgcaccgtc ctggcggggt cgagctgcgg
     6241 ctcctgctcg aggtagccga cccgggcgcc ctcggcgacc cacgcctcgc cgttatattc
     6301 cttgtcgagg ccggccatga tccgcagcag cgtcgatttg ccggaaccgt tgacgccgag
     6361 cacgccgatc ttggcatccg gataaaacga caggtggacg ttatcgagca ccttgcgggt
     6421 cgggtaggtc ttggacagac cctgcatgaa atagacgaat tggcgcgcca tcgcggtccc
     6481 ttaaaacgag aggattcatg gaaatttgct gccgctgatg tagcggcgcg ccccctaaag
     6541 ggcaaccgca ccccgacgtt ttccatccgt tcaccacgta tcaatacggg attgacacca
     6601 tgtgtgcgcc aagtcacgac aattgaaacc atcttttaat aaattgggcc aaagctgtcg
     6661 atcggcgcct cgaaaggcgt tccgcgcggc agaagtcgcg aacgaaaaca gcagggccgc
     6721 gtcatgacga ccatcacgtc agaaaccctc tcctccacga ctggcagccg cgacggctcc
     6781 agcctgttcg cagaaatccg tgccttctgg cgccagtttt tggcccgggc attcgacccc
     6841 taccggccgg aactgcatta catgcgcggc ccgggccccg cctggcacgc caagcagcag
     6901 cggtcgccgc gcgcccattc cgcctgatcc tgtcggatat tttgaaccgc gaacgctcct
     6961 gcagcgggct gtctttggcc ggcgcaggct ttttgcgcgg cgaacgcttg cgcgttttgg
     7021 aattgcgcgc aaccatggtg gttcccgggc ggctcgcgtc ttaagccgcc ttcatcgcca
     7081 tgaacggaac tcccatgacg cggctgcgct gtgcaattct cgatgactac ctcaatcttt
     7141 ccttgaccgc cgcggactgg tcgaagatcg gcgatcgggt cgacgtcacg gtattcaacc
     7201 agccctttgc ttcacccgag gccgccgccg gcgcgctgaa ggatttcgag atcatctgcg
     7261 cgatgcgcga gcgcacgccg tttccacgta cgctgtttgc ggcattgccc aaactgaaac
     7321 tcctgatcac ctcgggcatg cgcaatgccg cgctcgatct ggaagccgcg aaagaccatc
     7381 aggtggtact gtgtggcacg caatgggccc gcgatcccac agcacccctg accatgggcc
     7441 tgatcctgga actgacccgc aatatcggcc gcgagaacgc gcgcatgcat gccggcgaac
     7501 ccttgcaaaa attcgtcggc atcgagatcg agggcaggac gcttggcgtg gtcggcctcg
     7561 gcaagctcgg cgccaaggtc tcggcgctcg ccaaagcctt cggcatgaac gtgatcgcgt
     7621 ggagcccgaa cctgacgccg gaacggtgca aggaggtcgg cgtcggctac gccaccaagg
     7681 aggaactgtt cgcaaccgct gacatcatca ccgtccatgt ggtgctgagc cagcgctcgc
     7741 gcggactggt cggggctgct gatctggcgc ggatgaagcc gacaagttac ctcgtcaaca
     7801 ccgcgcgcgg gccgatcgtc gacgaagccg ccctgctgga aaccctgcag cggaagaaga
     7861 tcgccggtgc tgcggtcgac gtgttctcgg tcgagccgct gccggtcgat catcccttcc
     7921 gcaaactcga caacatcgtg ctgacgccgc atctcggcta cgtcaccgaa gaaggctttc
     7981 gcaaccacta cgtccagatg gtcgaaggca tcgacgcctg gatcaagggc gagccgctgc
     8041 ggcggctggt ctgagatcgg cagcggcggc agcttgagat cgccggcggc tcgctgacct
     8101 cgccgtcatg ccgcggacct acgttcgaag gatggcttcg cgatttccgc aaatccttcg
     8161 cgccatgatg gatgagccgg ccaccaaccg agatccgttt ttgcctttgc attcgatcca
     8221 gcccggacct gcgtcatcat agcaaccata tgttcgcccg ccacaagccg ggcgatccaa
     8281 gccggcacat gaaacggcgg cttggcgcca agcatctccg caagtgcggg aagccattcc
     8341 ctgacctggg cagggtgatc gtccacgatg ttgtagatgt tgccggcctt gccgcgctcg
     8401 atagccgaga ccgttgccgc agcggcatca tcgacatgta cgaacgacca ccatccgcta
     8461 ccgtcaccga tcagcggcac gcggcgatga cggagctgtt cgaccatagg gcggctcagc
     8521 atgccggtgt cgggtccgta gaacgatcca tatcgtagca cgatgccttc ggggttcgtc
     8581 gaatcggtga cggtccgctc caggtattgg atcgcttcaa gcgtgcggcg gagttcctcc
     8641 ggcggatctg gatcgagcgc gtccgcttct gatttgacgg gaccgcctgc tcgcgcgaaa
     8701 gcccaaccac agaagctttg ggcgatgaac cgtttgacgc cggcttcgcg cgcggccgcc
     8761 agaagaaggt cggttccgcg ggtcctcaac cggttggtgt tcgcgaacga acgatcgaaa
     8821 tgtctgaaat tggtgacgtc gctgagatcg gtcatttcat caatgacgat gtcgggtttg
     8881 gtggaagtta cggcagcgcc aaccgccccc ccatcgagcc cgtcggccac aacgggttcc
     8941 gcacccattt gcttgatggt ttcgcgcttt gcggccgtgc gggtgagccc cacgaccgaa
     9001 tggccgcttg cgaccagtgc gggaacaagc tgacggccca cggcgccggt ggcgcctgca
     9061 acaaagactc tcatgttgag ctcctgtatt cgctgcaact gcgcggcagg tagtttgagg
     9121 aaatgtgaat tcaaccatgc gaatgcaaaa tcctggggga caggaaccca agatgtccag
     9181 aacctcgtaa ttccacgtcc tgcgctgccg tcgccgaatc gcatcgccat tattgagagg
     9241 gtgcgatcat gaaaatgcgc cggaaccaac ccggttcctc gcggatcgaa acgaagccat
     9301 ccaactgaca agtgttcaga cataatcgga cgcaagagcg agccgctgcg tcgattgact
     9361 agccatcggc aaaacgcgcg gtcatccccg gatgagcgca attgaactca ctcgggaggt
     9421 gcgccgtctt cggcgagcct cgaaggatga ttgtgcctgt ggccatcctt cgaggcgcgc
     9481 aagggcgcgc acctcaggat t
//
LOCUS       DATNOI010000110         5871 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_821842,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000110 DATNOI010000000
VERSION     DATNOI010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821842"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VN968_03895"
     CDS             <1..333
                     /locus_tag="VN968_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28419.1"
                     /translation="LEAIDRPLDPRTAIKLNDGNGRGSCVAVVKRSQAKEKSLSAMLD
                     EAKGANCEGALSVQLTPGTTPGQVGNAQFTLLDFKRMISAAAIDDNALVAEKNERNPT
                     PQGNAPKL"
     gene            complement(489..2087)
                     /locus_tag="VN968_03900"
     CDS             complement(489..2087)
                     /locus_tag="VN968_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HXL28420.1"
                     /translation="MHWSHDSIPAMRLEARFGDRVVPAFCERPKSIWAMVSEAAARNP
                     DGEALVCGARRMTWREVAQQSACVAAGLRKMGLRSGDRVALLLGNRIEFVLAMFGAAH
                     LGLVTVLLSTRQQKPEIAYVLSDCGAKLLIHEAKLADRLPDARDVPELMHRISVDDGS
                     ASQFLGLLDNEASPQPAFVGEEETAMILYTSGTTGRPKGAMLAHCNVVHSSMIFVSCL
                     QLTAADRSIAAVPLGHVTGVVANIMTMVRCAGALIIMAEFKAADYLKVAVRERVTYTV
                     MVPAMYNLCLLQPDFDSCDLSSWRIGGFGGAPMPIATIEKLNATIPGLKLMNCYGSTE
                     TTSPSTIMPGELTAGHIDSVGLPCPGAHIMVMDADGHELPRGEIGEIWIHSGSVIKGY
                     WNNPTATAESFTGGFWHSGDLGSIDDENFVRVFDRQKDMINRGGLKIYSAEVESVLAG
                     HPDVVESAIIAKRCPVLGERVHAVIVTRHADVSGEALRAWCAERLSDYKVPETMALTV
                     EPLPRNANGKVMKRQLREAWTAAQ"
     gene            complement(2099..2922)
                     /gene="panB"
                     /locus_tag="VN968_03905"
                     /pseudo
     CDS             complement(2099..2922)
                     /gene="panB"
                     /locus_tag="VN968_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502047.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
     gene            complement(2924..3508)
                     /locus_tag="VN968_03910"
     CDS             complement(2924..3508)
                     /locus_tag="VN968_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HXL28421.1"
                     /translation="MGLLGMILGLVLFFGVHTLTTQREVRARVIAFSGGGGYKIGYSL
                     VSALGLVLIVWGFAHYRATGMWEIWTPPTALKHLTAALMLPAVILVVASYIRGRIYTR
                     LKHPMLTGIKLWAAAHLLANGDLGSIILFGSFLGWAVFDRISLKHRADSGAPPIPVGG
                     PSNDLIAVAVGIVAYLALAFAFHPVVIGVPVFGV"
     gene            complement(3751..4356)
                     /locus_tag="VN968_03915"
     CDS             complement(3751..4356)
                     /locus_tag="VN968_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28422.1"
                     /translation="MLRRVVICLIVAIAGIAVAGTADARQRNQINPTPFSHAPCSVLD
                     GRPCTPSFCSVFNHGPCIPEIDYPYGENLQLTVESAPSRDDAAKYQKPDHDLDTIGDL
                     FAALRSCWSPPSAASAREGMQMTVRFSFKRTGEIIAAPRLTFATAGVSADTRTIYLKA
                     INASLDACEPLKFTSGLGGALAGRPIAIRYVDNRALNQSSR"
     gene            complement(4426..4983)
                     /locus_tag="VN968_03920"
     CDS             complement(4426..4983)
                     /locus_tag="VN968_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL28423.1"
                     /translation="MTAKPRRATYRHGNLKAAALKAAFALVAEGGHEQLSLREVADAV
                     GVAHRSLYNHFEDREALLDAVATDAYTRLAAVLVKAQTPQDYTAKYVRFALANRAIYA
                     LMTSRPHAIMKHNPPLQSAVHKVITEAMRIFCRDIETPAERRRAVMKIYITLYGGVSL
                     YAAGVLDQPSEKALIAELSAMNAGM"
     gene            complement(5045..5770)
                     /locus_tag="VN968_03925"
     CDS             complement(5045..5770)
                     /locus_tag="VN968_03925"
                     /inference="COORDINATES: protein motif:HMM:NF004858.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXL28424.1"
                     /translation="MKDLVTYSRSGPVSTIVMDDGKANVMSLAMLNALHLAFDQAEKD
                     NTVAILKARGKHFSGGFDLNVFTNGNAEDQYLMLKAGAELALRILSFPTPVVAACQGN
                     AFPMGAFLIMSADHRIAAEGNYRIGMNEVAIGLTVPRFAIEIARQRLTPAYFSRVVIT
                     AEMFAPPEAVTAGFFDRVVAASELERAAEEAAHALSTLNMAAHAATKARARGAVISMI
                     RGMIDEDITPQYGRDRVASRASA"
BASE COUNT         1148 a         1887 c         1811 g         1025 t
ORIGIN      
        1 ctcgaggcga tcgaccggcc gctcgatccg agaaccgcga tcaagctcaa cgacggcaat
       61 ggccgaggca gctgtgtcgc cgtcgtgaaa cgctcgcagg ccaaggagaa atcattgagc
      121 gccatgctgg acgaagcaaa aggcgccaat tgcgagggcg cgctcagcgt gcagctaacg
      181 ccgggcacga cgccaggcca ggtcggcaat gcgcaattca cgctattgga tttcaaacgc
      241 atgatcagcg cggcggcgat cgacgataat gcgctcgtcg cggaaaagaa cgagcgcaac
      301 ccgacaccgc agggcaacgc gccaaaactt tgacgccttg gttcaacgcc cccccgctcg
      361 cggatggcgc gcagcgaaat ccggggactg tatcgccaac gggtgaccta tcccggattg
      421 cgcggagcct gtcatcgggc ggcgcttcgc gccgacccgt tggcttcatc cgggctacgg
      481 gctacgggct attgcgccgc cgtccatgcc tcgcgcaact gccttttcat caccttgccg
      541 ttggcgttgc gcggcaacgg ctcgaccgta agcgccattg tctccggcac cttgtagtcc
      601 gaaagccgct cggcgcacca tgcgcgcaag gcctcgccgc tgacgtcggc gtgccgggtc
      661 acgatcacgg cgtggacgcg ctcgcccagc accggacatc gttttgcgat gatcgcgctc
      721 tcgacgacat cgggatggcc cgccagcacg gattcgactt cggcggaata gatcttcaat
      781 ccgccgcggt tgatcatgtc tttctggcga tcgaacacgc gcacgaaatt ctcatcgtca
      841 atcgaaccga gatcgcccga atgccagaac ccgccggtga aactttccgc cgtcgccgtc
      901 gggttgttcc agtagccttt gatgaccgag ccagagtgaa tccagatctc gccgatctca
      961 ccgcgcggca actcatggcc atcggcgtcc atcaccatga tatgcgcgcc cggacacggc
     1021 aaaccgacgc tgtcgatatg gccagcggtc aactcgcccg gcatgatggt cgaaggcgac
     1081 gtggtttcgg tcgagccgta gcaattcatc agcttcagac ccggaatcgt ggcgtttagc
     1141 ttctcgatcg tggcgattgg catcggagcg ccgccaaagc ctccgatgcg ccagctcgac
     1201 agatcgcagc tgtcgaaatc cggctgcaac aggcagagat tgtacatcgc cggcaccatg
     1261 accgtgtagg tgacgcgctc gcgcacggcg accttcagat aatccgccgc cttgaactcg
     1321 gccatgatga tcagcgcgcc tgcgcaacgg accatggtca tgatgttggc gaccacgcca
     1381 gtgacatgcc cgagcggcac ggcggcgatc gaacgatccg ccgctgtaag ctgcagacag
     1441 gagacgaaaa tcatcgacga gtggacgacg ttgcaatgcg cgagcattgc ccccttgggc
     1501 cgcccggtgg tgcctgacgt atagaggatc atggcggtct cttcctcgcc gacaaaagcg
     1561 ggctgtggcg aagcctcgtt atcgagcaag cccaggaatt gcgacgcgct gccgtcgtcg
     1621 acggagatgc gatgcatcaa ctcgggaacg tcgcgggcat cgggcagacg gtcggccagc
     1681 ttagcctcgt ggatcaagag cttggcaccg cagtctgaca gcacatacgc aatctccggc
     1741 ttttgctgac gggtgctgag cagcaccgtc acaaggccaa gatgcgcggc gccgaacatg
     1801 gccagcacga attcgatacg gttgccgagc agcagcgcca cgcggtcgcc gcttcgcagg
     1861 cccatcttcc gcaatcccgc ggcgacgcat gcggattgtt gcgcgacctc acgccacgtc
     1921 atccgcctgg cgccgcagac cagcgcctcg ccgtccggat ttcttgcagc agcttctgac
     1981 accatcgccc agatgctttt gggccgttcg caaaacgccg gcaccacgcg atcgccgaag
     2041 cgcgcctcaa gccgcatggc gggaatggaa tcgtgcgacc agtgcattgt cgatgccttt
     2101 agctcttcgc cttcatgcca taaacatgtt ccggacccgg gaaagcgcgg gcgcgcacat
     2161 cggtggcata gccctcgacc gcggcctcga tcatgggtgc gaggttgccg tagcggcgga
     2221 caaatttcgg cgtccgcgcc gacaagccca gcatatcctc cagcaccagg acttgcccgt
     2281 cgcaggccgc gctggcgccg atgccgatgg tgggaatggc gatggtttcg gtgatcttcc
     2341 tggcaagggg ttctgcgacc gcctcgatca ccaccgagaa tgcacccgcc tccgagatcg
     2401 ccttggcgtc gttctcgatc ggctcccagc tgccctcctc gcggccctgg gcgcgaaacg
     2461 agccgagcgt gttgatcgac tgcggcgtca acccgatgtg gcccatcacg ggaacaccgc
     2521 gctcgacaag aaaccgacgg tctccgccat ccgcgccccg ccttcgagct tgaccgcgcc
     2581 gcaatgggtt tccttcagga ttcgcaccgc cgaatgaaac gcctgctcct tcgaagcttc
     2641 ataggagccg aacggcatgt ccaccacgac cagcgcatgt tgcgagccgc gcatcaccgc
     2701 gcagccttgc aggatcatca tgtcgagcgt caccggcacc gtggtctcga aaccatgcat
     2761 cacattgccc agggagtcgc cgaccagaat gacgtcgcaa tagcgatcga ccagtgcggc
     2821 ggtatgcgcg tgataagagg tcagcatcac gatcggctcg ccattcttgc gggcgcgaat
     2881 gtccggcgcg gtcttgcgct tgatggccga ttggacagac atttcagact ccgaacacgg
     2941 gaacgccgat caccacggga tgaaacgcga aagccagcgc cagataggcg acgatcccga
     3001 ccgcgaccgc aatcaggtcg ttgctggggc cgccgaccgg gatcggcggc gccccgctgt
     3061 cggcgcgatg cttcagcgag atccggtcga acaccgccca tccgaggaac gaaccgaaca
     3121 ggatgatcga gccgagatcg ccattggcga gcagatgcgc cgccgcccac agcttgatgc
     3181 ccgtcagcat cggatgcttc agcctggtgt agatacgtcc gcggatgtag gacgccacca
     3241 ccaggatgac cgcgggcagc atcagcgccg ccgtgaggtg tttgagcgcc gtcggcgggg
     3301 tccagatctc ccacatgccg gtcgcgcgat aatgggcaaa accccagacg atcagcacga
     3361 gccccaaggc cgaaacaagc gaatagccga tcttgtaacc gcccccgccc gagaatgcga
     3421 tcacacgcgc gcgcacctcg cgctgcgtgg tcagggtgtg aacgccgaaa aacagcacca
     3481 ggcccaggat catccccagc agtcccatgg tcttctccgt tacagggcca gccttccctg
     3541 cttcgcgtat cactttagcg gctcaggccg caatgcgcta aaatgcgttg ggacaggcgc
     3601 ggaacatttg agacaccggg cgattgcctc ttcgactcat aaggagagac agtgaaaaaa
     3661 ggcggccgat ttggccggct ttttttgtgg acggaaagaa agggcgccgg aggggagacg
     3721 gacctgagat ttttgacaac acccgattat ttagcggctt gattggttca acgcgcgatt
     3781 atcgacatag cggatcgcga tcggccgccc cgccagcgcg ccgccaagcc cgctggtgaa
     3841 tttcaacggc tcgcaggcgt cgagcgacgc gttgatcgct ttcagataga tggtgcgggt
     3901 gtcggcggaa acgcccgccg tggcgaacgt caggcgtggg gccgcaatga tctcgccggt
     3961 tcgtttgaag ctgaatcgca ccgtcatctg catgccttcg cgcgcggaag cagccgacgg
     4021 cggcgaccag caggaccgca gcgccgcgaa gaggtcgccg atggtgtcga gatcgtgatc
     4081 gggcttttgg tatttcgcgg cgtcgtcgcg cgacggcgcg ctttcgaccg tcagctgcag
     4141 gttctcgcca tagggatagt caatctcggg aatgcacggc ccgtgattga acacgctgca
     4201 gaacgacggc gtgcatggcc gaccatccag cacgctgcag ggcgcgtgtg aaaacggcgt
     4261 gggattgatc tgattccgct gccgggcatc agccgttccg gcaacagcga tcccggcaat
     4321 ggcaacgatg agacagataa cgacgcgacg gagcatgaac ccagccctcg aaggcaacac
     4381 accgaaaatg aggcgcgcag cggccggaaa caagcccggc ttcattcaca tccccgcgtt
     4441 catggctgag agttccgcga tcaacgcctt ctcgctgggc tggtcgagca cgccagccgc
     4501 atagagcgaa acgccgccgt agagcgtgat gtagattttc atcaccgcgc ggcgccgctc
     4561 cgccggcgtt tcgatatccc ggcagaaaat ccgcatcgcc tcggtaatga ccttgtggac
     4621 ggcgctctgc aaaggcggat tgtgtttcat gatcgcatgc ggccggctgg tcatcagcgc
     4681 atagatcgcg cggttggcga gcgcgaaacg cacatatttc gcggtgtagt cttgcggggt
     4741 ctgcgctttc acaagcaccg ccgcgagccg cgtgtaggcg tcggtggcga ccgcgtccag
     4801 caacgcctcg cggtcttcga aatgattgta gagcgaacgg tgcgcgacgc cgacggcgtc
     4861 ggcgacctcg cgcaggctca gctgctcatg accgccctct gccaccagag caaaggcggc
     4921 tttgagggcc gcggccttca aattgccgtg gcgataggtg gcccggcgcg gttttgctgt
     4981 catgcgcctt catacaaagc gaccggccaa aatgccacct cccggacgcg cagcggccct
     5041 tccctcacgc cgacgcacgg ctggccacgc gatccctgcc atattgcggg gtgatgtctt
     5101 catcgatcat gccgcggatc atgctgataa cggcgccccg cgcgcgcgcc ttggtcgcgg
     5161 cgtgggccgc catgttgagc gtgctcagtg cgtgggccgc ctcctcggcg gcgcgctcca
     5221 gctcactggc ggcaaccacg cgatcgaaga aaccggcggt cacggcttcc gggggtgcga
     5281 acatttccgc tgttatcacc acccggctga aataggccgg cgtcagccgt tgccgcgcga
     5341 tctcgatggc aaaacgcggc accgtcagcc cgatcgcgac ctcgttcatg ccgatccggt
     5401 aattgccctc ggcggcgata cggtgatcgg ccgacatgat caggaatgca cccatcggga
     5461 aggcattgcc ctggcaggcg gccaccaccg gcgtcgggaa cgacaggatg cgcagcgcca
     5521 gctcggcgcc ggctttcagc atcagatact gatcctcggc gttgccgttg gtgaatacgt
     5581 tgagatcgaa accgcccgaa aaatgcttgc cgcgcgcctt gaggatcgca acggtgttgt
     5641 ccttttcggc ctggtcgaac gccaagtgca gcgcgttcag catcgccagc gacatcacat
     5701 tggccttgcc gtcgtccatc acgatggtgc tgaccgggcc ggagcgggaa taggtcacga
     5761 gatctttcat actggcctcc ctcgaaacat ccactggata ctttagcaaa atcgcggaag
     5821 cttcaagggg atttatgggg ggatgcctgc gcatggaggc atccgctgtg c
//
LOCUS       DATNOI010000111        11621 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1384863,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000111 DATNOI010000000
VERSION     DATNOI010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11621
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384863"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..795)
                     /locus_tag="VN968_03930"
     CDS             complement(82..795)
                     /locus_tag="VN968_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28425.1"
                     /translation="MRKEVILSTLALRAGYGGKPVLQDLEIEVREGEIVAVIGRNGVG
                     KSTLMKSLIGLVPAMSGSIVYRGEAVELVPAHKRARLGMGYVPQGRDVFPRLTVAENI
                     AVGAAVKGGIPETGRRRIVEAFPILAERWSQRAGTMSGGQQQQLAIGRVLIADPNLIL
                     LDEPSEGIQPNIVQDIARYMVELNRTTGVTIILVEQNLDMIRAMAQRCYVMDKGRIVA
                     ALDRTALDDQAEMRRHLAV"
     gene            complement(788..1504)
                     /locus_tag="VN968_03935"
     CDS             complement(788..1504)
                     /locus_tag="VN968_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28426.1"
                     /translation="MSLLELHGLNKHFGGLHVTNAVNLSLEAGEIHCLIGPNGAGKST
                     LFRLILGEHHPGSGDIFFAGENITALKSFARIHRGLSVKFQVPGVFKGLSVRQNLEIA
                     LQRQHRGVELAGEIDRLLAFVNLEADQMQTAGNLSHGQKQWLEIGMAISLKPRLLLLD
                     EPTAGMSPEETHLTGEMVQRLNAEGMTVLAIEHDMAFVRQVAQRVTVLHLGQVFAQGS
                     IEEIVADERVVAIYLGQAHA"
     gene            complement(1504..2565)
                     /locus_tag="VN968_03940"
     CDS             complement(1504..2565)
                     /locus_tag="VN968_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL28427.1"
                     /translation="MNGFLALFRRLEGPQTAGRGPAFWIVFAIVLAIACAYPLFSDGY
                     TVGNTVYFFIWVFIALSLCLIWGYGGSLSFGQTAFFGIAGYSYGILTLNFGSAYGFTW
                     IALLLAIALAALFALLLGYFMFFGRIAGVFLGIVTLAVTLMLERFMAQTAGPEWHIGV
                     ARLNGFNGMSAMPPLTIPWPGGDIVLFADIGLYYAVLGLLVVVYLGLRILMNSAFGNV
                     IVAIRENPERAEMLGYDVRKYQLITFVIGAALAGLSGVLYTVWGQYITPSSMGMTAAA
                     LPLIWVAVGGRSDLTSTLIGTLVVLAAFQALTIYGSQYALVFMGVLLVLTVLIAPNGL
                     VLGVMNLISRLAARSNGDA"
     gene            complement(2579..3451)
                     /locus_tag="VN968_03945"
                     /pseudo
     CDS             complement(2579..3451)
                     /locus_tag="VN968_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969320.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
     gene            complement(3558..4796)
                     /locus_tag="VN968_03950"
     CDS             complement(3558..4796)
                     /locus_tag="VN968_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HXL28428.1"
                     /translation="MTRKVLRGLRAVALMGTLVLASGAAFAEDPIKLGLLEDQSGDFA
                     AATIGKVHAIQLAAEEINKAGGIAGRPLELVTYDTQSDNTRYQEFMRRVLQRDKVDVV
                     FAGFSSASREAYRPIVDQFNGFAFYNNQYEGGVCDGHMVVTGAVPEQQFSTLIPYMME
                     KYGKNVYTLAADYNFGQISAEWVRKIVKEAGGKMAGEEFIPLGVSQFSQSIQNIQKAK
                     PDFVVTLLVGTAQASYYEQAASANVNLPMASSVNVGQGYEHKRFKPPSLKDMYVTTNY
                     IEEIDTPSSKAFFAKFKAKFPSEPYVNQEAENSYLAVYLYKQMVERAKSTKREDIRKV
                     IALGDVCMDAPEGKVCMDPKSQHMSHTIYLAKVGPDHSISFPKVWEDIKPYWLGEAGC
                     DLTKKDPMAQYTPSNPPPKP"
     gene            complement(4808..5473)
                     /locus_tag="VN968_03955"
     CDS             complement(4808..5473)
                     /locus_tag="VN968_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HXL28429.1"
                     /translation="MTCRLLQNFKGGRALVVTGRSGREGALESTLAKLGVTTEYPDIV
                     GGRAQIDVGSLQPERDILFVDGDLEGAVAIEVNPASRLPPVPVIGLVGVEAPSRLKAL
                     VNLGATSFLRKPVHGGAVYTSLFMGINQFLLRSDMYARVQDLEGRRRGRRAVIRAVIL
                     LMQENGLDEESAYSQLRRASMRARQNLELYCEEFLSRRANPPDIPGRATSTTLQGGTK
                     QAM"
     gene            complement(5470..6630)
                     /locus_tag="VN968_03960"
     CDS             complement(5470..6630)
                     /locus_tag="VN968_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXL28430.1"
                     /translation="MARKHYRIGVMFSTTGSYSVVARSMLNGALLAFQENAEAAGAVA
                     LEPVVVNPSGDLACYRALSLELLGAGIRQVVGCYTSSSRKEVIPCFEKFDGLLWYPSH
                     YEGFESSDNVIYTGASPNQHVLPLVDYLASRVGTRAFCVGSNYIWAWENNRIFREALT
                     ARGGTVVAERYLSVGDTEFDQVISAILDQRPDFVFNNLIGTSAYAFFRAFRAACRARG
                     IDQVNEIPVASCTLSEPELAEIGADAVDGHLSSSVYFSSLESSANAAFIEAYAKAFPD
                     GPVSSADAEASYIAVKLLAATLEQAGIDDARTVRAAVANQRLAAPQGEVRIDPQTHHA
                     WLTPRIARSAVNGQFEVLLEARSPIAPDPYLVQSSPRFAGAMRSPVLRVVQT"
     gene            complement(7169..7513)
                     /locus_tag="VN968_03965"
     CDS             complement(7169..7513)
                     /locus_tag="VN968_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL28431.1"
                     /translation="MRKAKRKYGCPVEFSIDLLSGKWKTVILSRLKVRPMRYGELRHD
                     IPSLSDKILTERLRDLCEAGFVRQERSGSKSRYCLTRRGEMLKPMLQSLYDWGQDNAD
                     SFGVRFLSVELN"
     gene            7619..9082
                     /locus_tag="VN968_03970"
     CDS             7619..9082
                     /locus_tag="VN968_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HXL28432.1"
                     /translation="MTHDSSIQEGHETSAGTPRIVIIRNGPCLLDGSIPVVDPLGVPV
                     AANAPVRLCRCGQSQTKPFCDNSHIERSFTDDKNPQRVTDKLDVHEGQQAYVFDNRGT
                     CAHSGFCTDRLSSVFHLRKEPFVSPSGARLDDLVNAVRKCPSGALGIGIDAVRDPNLS
                     DTKRPPQIEVTRDGPYRVTGNVRLVDENGVLIPQNEGASREHFSLCRCGSSLNKPFCS
                     GMHWSVEFQDPVPDPLREPTLFEWVGGYPALLDMTRIFYSRYVPGDPLLGPLFAEMSP
                     DHPERVAAWLSEVFGGPPFYTERYGGYRRMVSQHIGKQIAPEQRARWVTYMLQSAEDA
                     ELPSDAEFRAAFVAYIEWGSRIAMENSGAGVKPPANMPVPRWWWVCNSTPGSRPTAKA
                     GNESGEVKTDPALPGADEAIRFEDHIRPLFRPLDRNSMLFAFDLWKEADLLKHGEQIL
                     LRLRAGTMPCDGAWPKEKIALFERWVAGGTADTASCD"
     gene            9485..9652
                     /locus_tag="VN968_03975"
     CDS             9485..9652
                     /locus_tag="VN968_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28433.1"
                     /translation="MQKDKSSQGIVLDEKSQEQPNDKERARTGSYAAAAGKEGRRASA
                     FEGEERVLGLR"
     gene            10014..10187
                     /locus_tag="VN968_03980"
     CDS             10014..10187
                     /locus_tag="VN968_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28434.1"
                     /translation="MYALFEGDRQIGSPFPTEKEVWEAALIEGLVTDVPLADEESGQV
                     IPVGYHVEHVADV"
     gene            10464..10937
                     /locus_tag="VN968_03985"
     CDS             10464..10937
                     /locus_tag="VN968_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017960985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL28435.1"
                     /translation="MAKNTICLWYDKDAEAAARFYAETFPNSAVGGVHRAPSDYPSGK
                     EGDVLMVEFTVAGVPCIGLNGGPAFKHDEAFSFQIATDDQQETDCYWNAIVGNGGQES
                     ACGWCKDKWGVSWQITPRVLTEALAAGGEEAKRAFDAMMGMKKIDVAAIKAARRG"
     gene            complement(11136..11303)
                     /locus_tag="VN968_03990"
     CDS             complement(11136..11303)
                     /locus_tag="VN968_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28436.1"
                     /translation="MNLFLVPTIGFKLLYGFVIVRIENLVWTNVTTNPTAERIARQFA
                     EAFLGTVPRVT"
BASE COUNT         2401 a         3541 c         3522 g         2157 t
ORIGIN      
        1 acgcccttgc tcgccatgat cgaattctcc tgccaggcca tcgatgctct ccttctctgt
       61 tgttaccgcc gtggaatggc ttcagaccgc gaggtgtcgg cgcatttccg cttggtcgtc
      121 gagcgccgtt cgatcgagcg ccgcgacgat gcggcccttg tccatcacgt agcagcgctg
      181 cgccatggca cggatcatgt cgagattctg ctcgaccagg atgatggtca caccggtcgt
      241 gcggttgagc tcgaccatgt agcgcgcgat gtcctggacg atattcggct ggatgccttc
      301 ggacggctca tcgagcagaa tgaggttggg atcggcgatc agcacgcgcc cgatcgcgag
      361 ttgctgctgc tggccgccgg acatggtccc ggcgcgttgg ctccagcgct cggcgaggat
      421 cgggaaagct tccacgatcc gccgccgtcc cgtctccggg attccgccct tgaccgcggc
      481 gccgacggcg atgttttccg caaccgtcag acgcggaaac acgtcgcgcc cctgaggaac
      541 ataccccatg ccgagtcgcg cccgcttgtg cgccggcacc agctcgactg cctcgccgcg
      601 atagacgatc gatccgctca tcgccggcac cagcccaatc aggctcttca tcagcgtcga
      661 cttgccaacg ccgttgcggc cgatcacggc gacgatctca ccttcccgca cctcgatttc
      721 gaggtcctgc agcaccggct tgccgccgta gccggcgcgc aaggccagcg tcgaaaggat
      781 cacttcctta cgcatgggct tgccccaaat agatggcgac gacgcgctcg tcggcgacga
      841 tctcctcaat cgagccttgc gcgaacacct ggccgagatg cagcacggtg acacgctgag
      901 cgacctggcg cacgaaggcc atgtcgtgtt cgatcgcgag cacggtcata ccctccgcgt
      961 tcagccgctg taccatctcg ccggtcagat gcgtctcctc cggcgacatg cccgcggtcg
     1021 gctcatcgag cagcaacagc cgcggcttca ggctgatcgc catcccgatc tcgagccact
     1081 gcttctggcc gtggctcaaa ttgccggccg tctgcatctg atcggcttcg agattaacga
     1141 acgccagcag ccggtcgatc tcgcccgcga gctcgacgcc gcggtgctgt cgctgcaagg
     1201 cgatttcgag gttctgacgg accgacagtc ccttgaacac accgggcacc tggaacttga
     1261 ccgacaggcc gcgatgaata cgcgcgaacg atttcagcgc ggtgatgttc tcgccggcga
     1321 agaagatatc gccgctgccg ggatggtgct cgcccaggat caaccggaac agcgtgctct
     1381 tgccagcacc gttcggaccg atcaggcaat ggatttcgcc ggcttcaagt gagaggttga
     1441 ccgcgttggt gacatgcaag ccaccaaaat gcttgttgag cccgtgaagt tccagaagcg
     1501 acatcaggcg tcaccgttgc tgcgcgcggc gagcctggaa atcagattca tgacgccgag
     1561 cacgagcccg ttcggggcga tcagcacggt cagcaccaaa agcacgccca tgaacaccag
     1621 cgcgtactgg ctgccgtaga tggtcagggc ctgaaatgcc gcaaggacca ccagcgttcc
     1681 gatcaacgtc gaggtgagat cgcttcgccc tccgaccgcg acccagatca gcggcagcgc
     1741 ggctgcggtc atgcccatgc tcgacggcgt gatgtattgc ccccataccg tgtagagcac
     1801 gccggaaaga ccggccagcg cggcgccaat tacaaaggtg atgagctgat atttgcgcac
     1861 gtcgtagccc agcatctccg cgcgctctgg attttcccga atcgcaacga tgacattgcc
     1921 gaacgcagag ttcattagga tgcgcaggcc tagatagacc accaccagca gcccgagcac
     1981 tgcgtaatag agaccgatgt cggcgaacag cacgatgtcg ccgccgggcc acgggatcgt
     2041 cagtggcggc atcgcgctca tgccgttgaa gccgttcagg cgcgccacac cgatatgcca
     2101 ctcggggccc gcggtctggg ccatgaagcg ctccagcatc agcgtcaccg ccagcgtgac
     2161 gatgccgagg aacacgcccg cgatgcggcc gaagaacatg aaatagccga gcagcaaggc
     2221 gaacagcgcg gcgagggcta tcgccagtaa cagcgcgatc caagtaaacc cataggccga
     2281 gccgaaattg agagtgagga tgccgtaact gtagccggca atgccaaaga acgcggtctg
     2341 gccgaaagag agcgagccgc catagcccca gatcaggcac aggctgagcg cgatgaacac
     2401 ccagataaag aaataaacgg tgttgccaac ggtatatccg tcgctgaaca gcggatacgc
     2461 gcaagcaatc gcgagcacga tggcgaacac gatccagaac gccggaccgc ggcctgcggt
     2521 ttgcggacct tcgaggcggc ggaaaagagc aagaaagccg ttcactgcgt cggatccttc
     2581 aggtgcgttc gcgcagcagg aagccggaga tccccttcgg caggacccga acgacgatga
     2641 tcacggcgac caagaggccg atctggccga acaactgccc ctgccaggat gtcatcgccg
     2701 acttcaccac tgcgagcacg ccaccggccg gcgccgtgcc gaggaacacg tcggcgccgc
     2761 cgatcaccac agtgacgaac gcttccatga tgaaagtggc gcccatggtc ggcaccagcg
     2821 tcatggtggg ggcgtacagg ccgccggcaa ggccggcgag cccggcgccg catgcaaagg
     2881 tgaggctgta gaccatgcgg gtatcgacgc cgagggccgc ggccatgtgg gggacctgga
     2941 tcgtggcccg ggcgagcacg ccgaagcggg tctagttgaa cagcatatag agaccgccca
     3001 gcacaccgag cgcggatgcg aacagcacga ggcgatagat ggaataggaa tagccgccga
     3061 cctgaaagct gccgaacggt gtaccgacgc cggccatggt ggatcccacc atgatcaagg
     3121 tgccctgcgt ggcaatcagg ctgaggcccc aggtcgccac gatcgtgtcg agcggccggt
     3181 cgtacaggtg acggatcacc acgagctcga ccagtgcgcc gaccagcccg gcgacgatag
     3241 cacccgtcat gatcccaagt ggaagcggca gaccggcctg gactgtcgat gccgtcacat
     3301 aggcgccgca catgatgaac tcgccatggg cgagattgat gacgcccatc atcccgaaga
     3361 tcaccgcgag cccgcatgcc gacagcacga ggaaggcgaa ggcatcgccg aattgataga
     3421 gcgccgagaa tgcaagcgtt gcgatgttca tggaccaacc gggatgatgc aaattcgaaa
     3481 ttcggtgaag cttcaacgat tcgagaacgc cgccccggca cgcaggagta cctgccgggg
     3541 cggcgccgac gccgaggtca gggtttcggc ggcgggttcg acggcgtgta ctgcgccatc
     3601 ggatccttct tggtgaggtc gcagccggcc tcgccgagcc aatacggctt gatgtcttcc
     3661 cacaccttcg ggaacgagat cgagtgatca ggcccgacct tggccaagta gatggtgtgc
     3721 gacatgtgct ggctcttcgg gtccatgcag accttgccct ccggtgcatc catgcagacg
     3781 tctccgagcg cgatcacctt ccggatgtcc tcccgcttgg tcgactttgc tcgttcgacc
     3841 atctgcttgt agagatagac cgcgagataa gagttctcgg cttcctggtt cacatagggt
     3901 tcgctgggaa atttggcctt gaacttggca aagaacgcct tgctggacgg agtgtcgatc
     3961 tcctcgatgt agttcgtggt gacatacatg tccttcaggc tcggcggctt gaagcgcttg
     4021 tgctcatagc cctggccgac attgacggac gaggccatcg gcaaattgac gttggcggac
     4081 gccgcttgct cataatagga ggcctgcgcg gtaccgacca gcagcgtcac tacgaaatcc
     4141 ggtttcgctt tctggatgtt ctggatgctc tgcgagaact gcgacacgcc gagcggaatg
     4201 aactcctcgc ccgccatctt cccgccggcc tccttgacga tcttgcgcac ccattccgcc
     4261 gagatctggc cgaaattgta gtcggcggcg agcgtgtaga cgttcttgcc gtacttctcc
     4321 atcatgtagg gaatgagggt cgagaactgc tgctccggca cggcgccggt aacgaccatg
     4381 tggccgtcgc agacgccgcc ttcgtactgg ttgttgtaga acgcgaagcc gttgaactgg
     4441 tcgacgatcg gacggtaggc ctcgcgcgag gcggacgaga agccggcgaa caccacgtcg
     4501 accttgtcac gctgcagcac ccgccgcatg aattcctggt agcgcgtatt gtcggactgg
     4561 gtgtcgtagg tgacgagctc gagcggccgg ccagcgatgc cgccggcctt gttgatctcc
     4621 tcagctgcga gctggatcgc atgcaccttg ccgatagtgg cggccgcgaa gtcgccggac
     4681 tgatcctcca gcaagccgag tttgatcgga tcctccgcga atgccgcgcc tgacgcgagg
     4741 acaagcgtcc ccatgagggc tacggcgcgt agcccccgca atactttcct ggtcatgtcg
     4801 gtgtctccta catcgcttgc ttggtgccgc cttgcagcgt ggtgctggtc gcgcggccgg
     4861 gaatgtcggg cggattcgcc cgcctgctca gaaactcctc gcagtaaagt tccaggttct
     4921 gccgcgcacg catgctcgcg cggcggagct gggaataggc gctctcttca tcgagcccgt
     4981 tctcttgcat cagcaggatg accgcgcgga tgaccgcgcg gcgaccgcgg cgacgacctt
     5041 cgagatcctg aacgcgggca tacatgtcac tgcgcagcag gaactggttg atgcccatga
     5101 acagcgacgt gtagacggcg ccgccatgga ccggcttacg caggaaggaa gtcgcgccga
     5161 gattgaccag cgccttgagg cggctcggcg cctcgacgcc gaccaggccg atcaccggca
     5221 ccggcggcag gcgcgaggca ggattgacct cgatcgcaac cgcgccctcg agatcgccgt
     5281 cgacgaacag gatgtcgcgc tcgggctgca ggctgccgac gtcgatttgc gcgcggccgc
     5341 cgacgatgtc gggatattca gttgtcacac cgagcttggc gagggtggac tcgagcgcgc
     5401 cttcccttcc gctgcgtccg gtgacgacaa gggcgcggcc gcccttgaaa ttctgcagca
     5461 atcgacatgt catgtttgca ccactctgag cacgggagag cgcattgcac ccgcaaagcg
     5521 cggcgacgac tggacgagat agggatcggg cgcgatcggg ctccgcgcct ccagcagcac
     5581 ttcgaattgc ccattgacgg cggagcgtgc gatccgcggc gtcagccagg cgtgatgggt
     5641 ctgcggatcg atgcgcactt ccccctgagg cgcagccagg cgctggttcg cgaccgcggc
     5701 gcgtacggtg cgtgcatcgt cgatgccggc ctgctccagc gtcgcggcca atagcttgac
     5761 cgcgatatac gacgcttccg cgtcggccga cgacaccggc ccgtccggaa atgccttggc
     5821 gtaggcttcg atgaatgccg cattcgccga cgattccagc gaggagaaat agacgctgga
     5881 gctcaggtgc ccatcgacgg catcggcgcc gatttcggcg agctccggct ccgacagcgt
     5941 gcagctggcc accggaattt cattgacctg atcgatgccg cgcgcgcggc aggcggcgcg
     6001 gaaagcgcgg aagaaggcat aggcgctggt cccgatcagg ttgttgaaga cgaaatcggg
     6061 acgctggtcg aggatcgccg atatcacctg gtcgaattcg gtgtcgccca cggacaggta
     6121 gcgctccgcg accacggtgc ctccacgcgc ggtcaacgcc tcgcggaaga tgcgattgtt
     6181 ctcccaagcc cagatgtagt tggagccgac gcagaatgcg cgggtgccga cccgcgaagc
     6241 gagatagtcg accagtggga gcacatgctg attgggcgat gcgccggtgt agatcacatt
     6301 gtccgagctc tcgaatccct catagtgcga cggataccag agcagcccgt caaacttctc
     6361 gaagcaggga atgacttcct tccggctcga agacgtatag cagccgacga cctggcgaat
     6421 gccggcgcca agcagttcca agctaagtgc gcggtagcac gcgagatcac ccgacggatt
     6481 gaccacgacc ggctccagcg ccacagcgcc ggctgcctcg gcattttcct ggaaggccag
     6541 caaggcgccg ttaagcatcg agcgcgcgac gacgctgtaa gacccggtcg tcgagaacat
     6601 cacgccgata cgatagtgct tccgcgccat ccgcccgatt ccaaacaaaa aagcgcccgc
     6661 cggctgtgaa gccgagggcg ccctcgccac caactggaca cgcttgaatt ctgaaagcgc
     6721 ggcgttggaa atggtttgaa cttgtagcgt tattgggcag ctttgcccaa cgcttgcatt
     6781 ttaggctccc ttagatcgac catgttgtca agcacgttgt tgcggcggga gttgcttcaa
     6841 ttgatgtctt gctgcagcaa aactatgcag ggtgccgcga taaactatcc cgttgctgga
     6901 agcgcctgaa cgtcgtcgtg atgtcgcgcg gtatcgaagt tgtgcggcca tgccgtgcgc
     6961 ctgcgcaaca ccagcctcgc taccttcagg agaggctgcg tcgaatggat ctagaaacgc
     7021 agcttgatga tacaagcatt ccgtcagccg tcttttccgc tattagctcc tcgcttccgt
     7081 aagctttttt gcgcgtgtcc gctttgcgcc agaacccgcc cacaaaatcg tttttccaat
     7141 cgacctgata tggtctgtct ttgcccgatc agttcagttc tacactgaga aaccggacac
     7201 cgaacgaatc ggcgttatcc tggccccaat cgtagagcga ctgcagcatc ggcttcaaca
     7261 tttcgccacg tctggtcagg caataccgcg atttactacc ggagcgttcc tgcctcacga
     7321 agccggcctc acagagatcg cgcagccgtt cggtcagtat cttgtcgctc agcgacggaa
     7381 tgtcgtgacg caattcgccg tagcgcatcg gccgcacttt cagcctgctg aggatcaccg
     7441 tcttccactt cccactgagg agatcgatcg agaattccac gggacagcca tattttcgtt
     7501 tggccttacg catgatccac gctccggttc tactttccat ctggttcgtt gaaggctgcc
     7561 atcaatccat ttaagcacgc ttttcgagtt cgttgaacga cgaggatcat tccaaagcat
     7621 gacccacgac agttccattc aagaagggca cgagaccagc gcaggaaccc cacggatcgt
     7681 cataatccgc aacggtccct gtttgctcga tggctccatc cccgtcgtcg accctctcgg
     7741 agttcccgtc gccgcaaatg caccggtccg gctgtgccgc tgcggacagt cgcaaaccaa
     7801 accgttctgc gacaatagcc acattgaacg cagcttcacc gacgacaaga acccacagcg
     7861 cgtaaccgac aagctggacg tgcatgaggg ccagcaggct tacgtgttcg acaaccgcgg
     7921 gacatgtgcg cattcgggtt tctgcacgga ccgcctcagc tcggtattcc acctccgaaa
     7981 ggagcctttc gtctcaccga gtggcgcgag actggacgat ctggtgaatg cggtgcgcaa
     8041 gtgtccttcg ggcgcgcttg gcattggcat cgacgcggta cgcgatccga acctttctga
     8101 tacaaaacgc ccgccgcaga tcgaagtcac cagggacggc ccttatcgcg tcacgggaaa
     8161 cgtcaggctg gtcgacgaaa acggcgtgct cattccacaa aacgaaggtg cttcccggga
     8221 gcacttcagc ctgtgccggt gcgggtcgtc cctcaacaag cccttctgca gcggaatgca
     8281 ctggagcgtc gaatttcagg atccagtacc cgatccactg cgtgagccaa cgttgttcga
     8341 gtgggtcgga ggatatcccg cgcttctcga catgactcgg attttctata gccgctacgt
     8401 cccaggagat ccgttgcttg ggccgttgtt tgcagaaatg tcgccagatc atccggagcg
     8461 cgtcgccgcg tggctgagtg aggtttttgg tgggccaccc ttctatacag aacgatatgg
     8521 cggctaccgg cgtatggtgt cgcagcatat tggcaagcag atcgcgccgg agcagcgcgc
     8581 gcggtgggtc acctacatgt tgcagagcgc cgaagatgcg gaactgcctt ccgatgctga
     8641 atttcgtgct gccttcgtcg cctatataga gtggggatcg cggatcgcca tggagaattc
     8701 tggcgccggg gtcaagccac cggctaatat gccggtaccg cgatggtggt gggtgtgcaa
     8761 ttcaacgccc ggttcacgtc ccactgcgaa ggctggcaat gaatcgggcg aggtcaaaac
     8821 tgatccggca ttgccaggcg cggacgaagc cattcgcttc gaggatcata ttcgcccgct
     8881 gtttcgtccg ctggatcgca actcgatgct gttcgccttc gacctctgga aggaggcgga
     8941 cttgctcaag cacggagagc aaattctgct aaggttacgt gccggcacga tgccatgcga
     9001 tggcgcatgg cccaaggaga agattgcgct cttcgaacgc tgggttgccg gtggaacggc
     9061 cgacaccgct tcttgcgact gaccatggcc cattttggcg gatgtcctcc ttgcgccagg
     9121 agcgggcttc cgagctacgc tgcaagagcg ccgatggcgg cttgacgccg aagtgtgcat
     9181 aggcgaacgc acgggcccag ctccttatac cgctggcgcg cgttctatgg cgcctacccc
     9241 ggccgccaat caggttcggt tgtgttcgta tcggtcgcga acgatctgat ttgtcacgcg
     9301 gtccctgcga ctgcgcgaca aggagcagaa acgcgccgat gaaataaaca gcatccatgt
     9361 tgaagactcc caaagcatcc cacaccgggg aagtttgtaa tgcgtgaaaa acccggaaaa
     9421 aaccccaggg ttcctatcga acctgactcc gccttttgcg ttggtttccg ataggaggtt
     9481 tgcaatgcaa aaggacaaat ccagccaagg catcgtgctc gatgaaaaat cccaggagca
     9541 gccgaatgat aaagagcggg cccgaaccgg gtcatatgcc gcagcagccg gtaaagaagg
     9601 acgacgagct tcagcctttg agggagagga gcgggttcta ggattgcggt gacttgaatt
     9661 ttgaaactac cgtgaggatg atgatttccc ggcacggctc gcaaaaaaat gcgctgccag
     9721 agttcgggtc atccggcttg gagcatcgcg ggcacacatg cccgcgaaac gtcgaaaacg
     9781 ccgctgcggt tcctactttg acaaactttt gaagaggaga gaccaatgta aagggcctat
     9841 cgcagtcgcc gcttttccgt gatgtgcgga tgatcgcgct tcgtacagaa caccggcccc
     9901 gctccgcagt ccggcataga acggcgccaa ggaaaaaaat ggctcgatgg acaccaccgg
     9961 catgagcaat aagggattat tctaactggc gggtttttga ttaggaggca cccatgtacg
    10021 cactgtttga aggcgacaga cagattggaa gccccttccc gaccgagaaa gaggtttggg
    10081 aagcggcgct gattgaaggc ctcgtgaccg acgtcccgct ggcggatgag gaaagcgggc
    10141 aagtcatccc tgttgggtat cacgtcgaac acgtcgccga cgtttaaccg cccatctgga
    10201 tgggtgactg agaattaagt cgatttgcct acctgaaagg cgatgacctc aaggcggcca
    10261 cctagtccct cagcgccacc gtgcttaagg atcaggtcga gataaagggc ggagagtcct
    10321 tcagcatcat ccccgatccg atcggggcca tgctgggcct atagtagccg aagaacgatt
    10381 cactactatg aacgcgtagt agatgcagca cggcatcgat agaaaaccga ggaaggcgca
    10441 taagttgagc gaaaggacac gcaatggcca agaacacgat ttgcctgtgg tacgataagg
    10501 acgcagaggc tgctgcccgc ttctacgccg aaacctttcc caacagcgct gtgggtggcg
    10561 tccaccgtgc acccagtgac tacccatccg gcaaggaggg agacgtgctg atggttgaat
    10621 tcacagtcgc cggcgtcccc tgtatcggcc tcaacggcgg tcccgcgttc aagcatgacg
    10681 aagctttctc gtttcagatc gccaccgatg atcagcagga gaccgactgc tactggaacg
    10741 ccattgtcgg caatggaggc caagagagtg cgtgcggctg gtgcaaggac aagtggggcg
    10801 tttcctggca aatcacgccg cgcgtgctga cagaggcgct ggcggccggc ggcgaagaag
    10861 caaagcgagc attcgatgcc atgatgggca tgaagaaaat cgacgtcgcc gcgatcaaag
    10921 cggcgcggcg cggctgatat taatcagaac ttccccggat aggtgtcttg gctgggctca
    10981 gtggtcgcgg gtgcgtctcg ggcatcacgg ccacgaggaa atgcagcgac gtggcgacgc
    11041 cattgctgca ccgcatgggc caggtttgtc ccgaatgctc atggcttgca atcggcgtgt
    11101 gacgacggcg ccatagatgc gatccctgtc tcggatcatg taacccgggg caccgtccca
    11161 aggaaagcct cggcgaactg ccgtgcgatc cgctccgccg tcgggttggt tgtgacattg
    11221 gtccaaacga gattctcaat ccgtacaatc acgaagccat acagcagttt gaagccaatg
    11281 gtcgggacca agaacagatt catggcggca atgtcgggag cgtggtttcg cagaaaagtc
    11341 ttccacccct aactcgcagg aaccagcggt cgttgttggt aagccgggct cgacccctca
    11401 ccttgcgccg caaaacgacc agctgatgtc ggggcaccgc actccgcctc aagcctgctc
    11461 ttcgactcga acggcgaggc cagcacggcc agaacaaaac agtacatggc gtcggccggc
    11521 ccgatcttct ccggcgaaca ttatcttcgg ccttgcccgc gcgtgcctgc acgagcaaag
    11581 cacgtcaaat cagtttcata gtcctttcac caaccactcg a
//
LOCUS       DATNOI010000112        10164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_503668,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000112 DATNOI010000000
VERSION     DATNOI010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10164
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503668"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VN968_03995"
     CDS             complement(<1..867)
                     /locus_tag="VN968_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HXL28437.1"
                     /translation="MAADNPHGALFRLSVPNQHSRVTYAELFFDLVFVFAVTQISHTL
                     IGRFTPLGALQTSLLFLAVWWVWVYTSWITNWLNPEVTPVRILLFLLMLGGLVLSTSI
                     PKAFESRGLWFAAAYAAMQVGKTVFLWVSTPPSRNLARLNAVRITAWLAMSAIFWIAG
                     GFAEGNSRLVLWAIALGIEYLSPAVRFWIPKYGASSIEDWLVEGGHIAERCAGFIIIA
                     LGESIVVTGATFADLAWTTETVVAFVSAFIGSLAMWWIYFHKGVEAGSELISKSSEPG
                     RLARLAYTYLHMP"
     gene            complement(891..1127)
                     /locus_tag="VN968_04000"
     CDS             complement(891..1127)
                     /locus_tag="VN968_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HXL28438.1"
                     /translation="MPMDAHDIESMIKAAIPDAEVTIRDLAGDGDHYAATVISESFRG
                     KSRVQQHQIVYQSLRGQMGGVLHALALQTGVPEG"
     gene            1308..1667
                     /locus_tag="VN968_04005"
     CDS             1308..1667
                     /locus_tag="VN968_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HXL28439.1"
                     /translation="MKLPGPDHPITITANPKRIRVSADGVVIADTTHALTLKEATYPA
                     VQYVPREDAKMALLARTDRVTHCPYKGDANYFSIMADGKTIENSIWTYETPFPAMAEI
                     SGHLAFYPDKVKIEEVG"
     gene            complement(1765..1938)
                     /locus_tag="VN968_04010"
     CDS             complement(1765..1938)
                     /locus_tag="VN968_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HXL28440.1"
                     /translation="MTILKWALIFFLVSIVAGILGFTGVSAASADIASFLFYVFVVIF
                     LVLLILGLTIFRV"
     gene            2165..3349
                     /locus_tag="VN968_04015"
     CDS             2165..3349
                     /locus_tag="VN968_04015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXL28441.1"
                     /translation="MTSISAVGHADVGAVPKAKSRNLSLDRARTFLTLVVLTHHAVIP
                     YTYFGHTDPKYFFGFDMIVLATDSFFMAMFFFLSGLFAWSGIARKGPLNYLADRLLRL
                     GLPFVICAFTVIPLAYYAISLRHHPEIGFSDYWWNMVTKGPWPSGPIWFLWVLFAFDL
                     VACLLHRLSPNLLDPINRLSLHGRRRPAVFFAVMLAVTAAFYIPGLVYYGPSSWFEFG
                     PFSVQHGRVMLYASYFFFGAGIGVAQMDRGLLAADGRMAKVSWDWMVLAIVPYCLLWV
                     LIFIKREILGNPSPLPNWYEALYAICFTVFSVAIMFLILAFFQRFRQSGSAKLLDPMQ
                     ADAYGMFLVHYPIALWLQYWLFDYDLPAIVKVAIGLVLTVALSWALTRALRKIPGATK
                     VL"
     gene            complement(3416..5623)
                     /gene="purL"
                     /locus_tag="VN968_04020"
     CDS             complement(3416..5623)
                     /gene="purL"
                     /locus_tag="VN968_04020"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HXL28442.1"
                     /translation="MNQPQITPELVASHGLKPDEYERILKLIGRVPSFTELGIFSAMW
                     NEHCSYKSSRLHLRGLPTKAPWVIQGPGENAGVIDIGDGQAVVFKMESHNHPSYIEPY
                     QGATTGVGGILRDVFTMGARPIACLNALSFGAPEHPKTRHLVSGVVAGVGGYGNSFGV
                     PTVGGQVRFHTRYDGNILVNAMAVGLADTDKIFYAAASGVNMPIVYLGSKTGRDGIHG
                     ASMASAEFDVDSAEKRPTVQVGDPFAEKLLLEACLEIMQADCVIAIQDMGAAGLTCSA
                     VEMGAKGDLGVDLDLDAVPTRETGMSAYEMMLSESQERMLMVLKPEKEKQAEAIFRKW
                     GLDFAVVGYTTPSKRFVVKHGGDVMADLPIKELGDEAPLYDRPHVASPLLPVVHARDV
                     KPPVAISAALETLIATPELCSKRWVWEQYDHVILGNTVQRPGGDAAVVRVQDGPKGLA
                     LTVDVTPRYCEADPFEGGKQAVAEAWRNITAVGGRPLAITDNLNFGSPERPEIMGQFV
                     GCLKGISEACRALDFPVVSGNVSLYNETNGRGILPTPSIGGVGLLDDFTKSATLAFKA
                     EGEAILLVGETHGWLGQSVYLRDICGREEGAPPPVDLAAEKRNGDVVRGMIHAATATS
                     AHDVSDGGLLIALAEMAMASGIGAQLLAAPGQIVPHAWWFGEDQGRYIVTVLAEQAGL
                     VLAKMNGAGVPCARIGTTGGDAIAVAGETPVAVSSLKSAFEHWLPAYMNGKAY"
     gene            5786..6280
                     /locus_tag="VN968_04025"
     CDS             5786..6280
                     /locus_tag="VN968_04025"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXL28443.1"
                     /translation="MDDLTKSAFLNRPDLHVETEGEFKGWRTWSRDNFESHNGPFWHR
                     MEDDGQIRCAFRVEKKHLNGMSNVHGGAFMAFADYCLFAMAAPVLQGPGVTVSFGCEF
                     LDAAREGELVEGTGDIMRAGGSMIFLRGLLKSAERPLFTFSGTIKRVKRRMPAAAAPS
                     AGSG"
     gene            complement(6272..6880)
                     /locus_tag="VN968_04030"
     CDS             complement(6272..6880)
                     /locus_tag="VN968_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28444.1"
                     /translation="MVELVPEMLSGPGAQQARRRVANRLDPEKLYRELSDRLAATDTI
                     ANRVALAAECLRIERFAEAERHYDHILQLPLGDDPVYALGKAKAQFGRDRPAESLATL
                     DDLQKRWPDFQSAEGHLLYARALGAVGRVDEALEEFHAVAAYFPGAEARVRYGLLLNL
                     VGRTAEARVVFNELLIQMRRAPKYLRQAQAEWLSIAEKQLSA"
     gene            complement(7146..8750)
                     /locus_tag="VN968_04035"
     CDS             complement(7146..8750)
                     /locus_tag="VN968_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL28445.1"
                     /translation="MLIHKASCTVALAAVLLSYQPVMAETTLRIAMTASDIPTATGLP
                     NNGFEGMRFLGYPIFEGLVLWDLTRTDRLAALRPGLAEKWEQDPEDNKTWIFHLRHGV
                     KFHDGTDFNADAVIWNLDRYFNSTSPQFEPPSSAMSRARVPVMGSYRKIDDATVAITT
                     TKPASYFPYMAVYLLFTSPASFEKAGGDWAKVATFPAAGTGPFRITRIVPRQEADLAR
                     WDKYWDPAKMAKVDNVVLMPIPEANSRLAALRSGQVDWIEVPPADGIASLKSAGFAIT
                     TGSYPHVWPWFYNIGAANSPFKDVKVRQALNYCIDREGLVSLLNGTAEPSAGWLKTGD
                     PDFGNPVNRYKFDPAKGKALLAEAGYTPAKPLSFKALISNSGSGQMLPLPMNEFLQQN
                     LKEACGVTVEFDVIEWQILLTAARATPDSPNLRGAMALNISSPSSDAGVMARYFLAAN
                     FSPNGFNFEQWKDDEFEDALKTISEATDPKVISTAYRKAHERLVDNPPWLYIVHDLNP
                     RAMSPKVKGFVSPQSWFVDLTLVSVQ"
     gene            complement(8989..9690)
                     /gene="purQ"
                     /locus_tag="VN968_04040"
     CDS             complement(8989..9690)
                     /gene="purQ"
                     /locus_tag="VN968_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HXL28446.1"
                     /translation="MKSAVLVFPGINRERDMARALKLISGHEPQMVWHAETALPAGTD
                     LVVVPGGFSYGDYLRCGAIAARAPIMDAVRAFAVQGGLVVGVCNGFQILCEAGLLPGV
                     LMRNARLKFICHDVHLRVERSDTPFTRGYNAGQVIRVPIAHGEGNYEADEQTLKRLEG
                     EGRVLYRYCSAEGVVDENSNINGAAQSIAGIVNERGNVLGMMPHPENHVEDIMGCTDG
                     RGLFAGLVAHLERAA"
     gene            complement(9704..9946)
                     /gene="purS"
                     /locus_tag="VN968_04045"
     CDS             complement(9704..9946)
                     /gene="purS"
                     /locus_tag="VN968_04045"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HXL28447.1"
                     /translation="MKARVTVTLKSGILDPQGKAIEGALKSLGVDGVASVRQGKVFDI
                     ELSGSDTAKAEAALKDAADKLLANTVIENYRVEVLS"
     gene            complement(9955..>10164)
                     /locus_tag="VN968_04050"
     CDS             complement(9955..>10164)
                     /locus_tag="VN968_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXL28448.1"
                     /translation="DMMRIIVADEISPDSCRLWDVKSNEKLDKDRFRRDLGGLLEAYT
                     EVAKRLGILMENERPIGSGPVLVKS"
BASE COUNT         1923 a         3228 c         3067 g         1946 t
ORIGIN      
        1 cggcatatgc agataggtgt aggccagcct cgccagccgc ccgggctcgc tggattttga
       61 aatcagttcg gaccccgctt cgacgccctt gtggaaatag atccaccaca ttgcgaggct
      121 gccgataaag gccgacacaa aggcaaccac ggtctccgtg gtccaggcca ggtcggcgaa
      181 cgtcgcgccg gtcacgacaa tggattcgcc gagcgcgatg atgatgaatc cggcgcagcg
      241 ctccgcgatg tgcccgcctt cgacgagcca gtcctcgatc gaagacgcgc cgtatttcgg
      301 gatccagaac cggaccgccg gcgagagata ttcgatgccc agcgcgatgg cccaaaggac
      361 cagccgcgaa ttcccttccg caaagcctcc cgcgatccaa aagatggccg acatggctag
      421 ccaggcggtg atacggaccg cattcaagcg cgccagattt cggctaggcg gtgtcgatac
      481 ccacagaaag accgtcttgc cgacctgcat cgcggcgtag gcggcggcga accacagtcc
      541 ccgcgattca aaggcttttg gaatcgaggt cgagagcacg agcccgccga gcatcagcaa
      601 aaacagcagg atgcggactg gcgtgacttc cggattgagc cagttggtga tccatgaggt
      661 gtagacccac acccaccaga cggccaggaa caacaggctc gtctgcaatg cccctagcgg
      721 cgtgaaccgg ccgatcaggg tgtgggatat ctgcgtgacc gcgaaaacga acaccagatc
      781 gaaaaacagc tcggcatagg tgacgcggct gtgctggttg ggcacggaga gacgaaacag
      841 cgcgccgtgc ggattgtcgg cagccatgga ctgaccctgc cccgcccccg ctacccctcc
      901 ggcactccgg tctgcaacgc cagcgcgtgc agcacgccgc ccatctggcc ccgcagcgat
      961 tgatagacga tctgatgctg ctggacacgg gatttgccgc ggaacgactc tgaaatcacg
     1021 gtcgcggcat agtggtcgcc gtcgccggcc agatcacgga tcgtcacctc ggcgtcgggg
     1081 atcgccgcct tgatcatcga ttcgatatcg tgggcgtcca tcggcatcca ggttttctcc
     1141 aattcggcac ttcggccagc ggccgacacc ggccgcaggg tcgccagaac ttagcgcgaa
     1201 cgcccttcta ggtcacgctt tgtcgcacta tattcccgat ccaatccttg caaaaccgct
     1261 ggatcgcatc ttgcgatcac aacggactga ccatgaggcg tacaaccatg aaactcccag
     1321 gtcccgacca tccgatcacc attaccgcca acccgaagcg gatccgtgtc tccgccgatg
     1381 gcgtcgtgat cgccgacacc acccatgccc tgacgctgaa ggaagcgacc taccccgcag
     1441 tgcagtatgt gccacgcgag gatgccaaaa tggcgctgct ggcgcgcacc gaccgggtca
     1501 cccactgccc ctataagggg gatgcgaatt atttcagcat catggccgac ggcaagacca
     1561 ttgaaaattc gatctggacc tatgaaacgc cgttcccggc gatggcggaa atctccggcc
     1621 atctggcgtt ttacccggac aaggtgaaga tcgaggaagt gggctgaggg tcaggggatc
     1681 gtagcccgga tgagcgaagc gaatccgggg tcggggtcac gttcgttccc ggatttcgct
     1741 tcgctcattc gggctacgcc cgcctcatac cctgaagatc gtgagaccca ggatcagcag
     1801 gaccaggaag atcacgacga agacatagaa caggaagctg gcgatgtcgg ctgatgcggc
     1861 cgaaacgccg gtgaagccga ggataccggc gacgatcgac accaaaaaga agatcagcgc
     1921 ccatttcaga attgtcatcg cccgtcactc cagcctgcgg gccgccggat ccgcggctcg
     1981 tacaaaaaaa atcgctgatc taacttcgag cagtggcact ggttccacaa tggatgaggc
     2041 gggcggcgtc gagatcgccg ccccgcccgg cgggcaattg cgttcgtcgc gccataattg
     2101 ggccttgctg aatccggcgc ccggccgcaa cattcggcac aaacagtctg gccgggaggc
     2161 gcgcatgaca tcgatttccg ccgtggggca tgccgacgtg ggcgcagtgc ccaaggccaa
     2221 atcgcgaaac ctttcgcttg atcgcgcccg caccttcctg accctggtgg tgctcaccca
     2281 ccatgccgtc atcccctata cctatttcgg tcacaccgat ccgaagtact tcttcggctt
     2341 cgacatgatc gtgctcgcca ccgacagttt cttcatggcg atgttctttt tcctgtcggg
     2401 actgttcgcc tggtccggta tcgcccggaa gggaccgttg aactatctgg cggaccgcct
     2461 gctccggctt ggcctgccgt tcgtgatctg cgcgttcacg gtcattccgc tcgcctatta
     2521 cgcgatctcc ctgcggcacc atcccgaaat cggcttttcg gattactggt ggaatatggt
     2581 cacgaagggt ccatggccga gcgggccgat ctggtttctc tgggtcttgt tcgccttcga
     2641 cttggttgcc tgcctgctgc atcggctgtc gcccaatctg ctcgatccga ttaatcgcct
     2701 ctcgctgcat ggtcgccgtc ggccggccgt gttcttcgcg gtcatgcttg cggtcaccgc
     2761 ggcgttctac attcccgggc tggtttacta cggaccaagc agttggttcg agttcgggcc
     2821 gttctcggtg cagcacgggc gcgtgatgct ctatgcgagc tacttctttt tcggtgccgg
     2881 catcggcgtt gcgcaaatgg atcgcgggct gctcgctgca gacggccgga tggcgaaggt
     2941 cagctgggac tggatggtgc tggcgatcgt tccgtattgc ctgctgtggg tgctgatctt
     3001 catcaagcgc gaaatactgg ggaacccgtc gccgttgccg aactggtatg aagcgctcta
     3061 tgccatctgc ttcaccgtct tcagcgtggc catcatgttt ctgatcctgg cctttttcca
     3121 gaggttcagg caatcaggct cagccaagct actcgatccg atgcaggctg acgcctacgg
     3181 catgttcctg gtgcactacc cgatcgcact gtggctgcaa tactggctgt tcgactacga
     3241 cctgccggcg atcgtcaaag tcgcgatcgg gttggtgctg acagtcgcgt tgagctgggc
     3301 gctgacgcga gcattgcgca agattccggg agcgacaaag gtgctctagg cttgtggccg
     3361 gatgagccaa cggggcgcgc attcgcgcga ccgttggctc atccgggctg cgaaatcagt
     3421 atgccttgcc gttcatataa gccggcagcc agtgttcgaa cgccgacttc agcgaactta
     3481 ccgcaaccgg tgtttcaccg gcgaccgcaa tcgcatcgcc gccggtggtg ccgatccgcg
     3541 cgcacggcac gccggcgccg ttcattttgg ccagcaccag gcccgcctgc tccgcgagca
     3601 ccgtcacgat ataacgcccc tgatcttcgc cgaaccacca ggcgtgcggc acgatctgtc
     3661 cgggtgccgc caatagctgc gcgccgatcc cgctcgccat cgccatctcc gccagcgcga
     3721 tcaagagacc gccgtcggaa acgtcgtgtg ccgatgtcgc ggtggccgca tggatcatgc
     3781 cgcgcaccac atcgccgttg cgcttttccg ccgcgagatc aaccggcggc ggcgcgccct
     3841 cttcgcggcc gcagatgtcg cgcagataaa ccgactggcc gagccagcca tgggtttcgc
     3901 cgaccaagag gatcgcctcg ccctcggcct tgaacgccaa ggtggcggat ttggtgaaat
     3961 catcaagcag cccgacgccg ccgatcgacg gcgtcggcag aatgccgcga ccgttggttt
     4021 cgttgtagag cgagacattg ccggacacga ccgggaaatc gagcgcgcgg caggcctccg
     4081 agatgccttt caggcagccg acgaactggc ccatgatctc cggccgttcc ggactgccga
     4141 aattgaggtt gtcggtgatc gccagcggcc ggccgccgac ggccgtgata ttgcgccagg
     4201 cctcggccac cgcctgcttg ccgccttcga acgggtcggc ctcgcagtag cgcggcgtca
     4261 cgtcgaccgt cagcgccaga cccttcggtc cgtcctgaac ccggaccacg gcggcgtcgc
     4321 cgccgggacg ctgcacggtg ttgcccagaa tcacatgatc gtattgctcc cacacccagc
     4381 gcttcgagca aagctccggc gtggcgatca gtgtttccag cgccgccgaa attgccaccg
     4441 gcggcttcac gtcgcgcgca tggaccaccg gcaacagcgg cgacgccaca tgcggccggt
     4501 cgtagagcgg cgcttcgtcg cccagttcct tgatcggcag atcggccatc acgtcgccgc
     4561 catgcttgac gacaaagcgc ttgctcggcg tggtgtagcc gaccacggcg aaatccagtc
     4621 cccatttgcg gaagatcgcc tcggcctgtt tttccttctc gggcttgagc accatcagca
     4681 tgcgctcctg gctttccgag agcatcatct cgtaggcgct catgccggtt tcgcgggtcg
     4741 gcaccgcatc gagatcgaga tcgacgccga gatcgccctt ggcgcccatc tccaccgccg
     4801 agcaggtcag gccagccgcg cccatgtcct ggatcgcgat cacacaatcg gcttgcatga
     4861 tctcgaggca ggcttccagc agcagctttt ccgcgaaggg atcgccgacc tgcacagtgg
     4921 gacgcttctc ggccgagtcg acgtcgaatt cggccgatgc catcgaggcg ccgtgaatgc
     4981 cgtcgcggcc ggtcttggag ccgagataga cgatcggcat gttcacgccg gacgccgccg
     5041 cataaaagat cttgtcggta tcggcgagcc cgaccgccat tgcattgacc aggatgttgc
     5101 cgtcatagcg ggtgtggaaa cgcacctgcc cgccgacggt cggcaccccg aacgaattgc
     5161 cgtagccgcc gacgccggcg acgacgcccg acaccaggtg ccgcgtcttg ggatgctccg
     5221 gcgcgccaaa gctcagcgca ttgaggcacg cgatcgggcg cgctcccatg gtaaagacat
     5281 cgcgcaaaat accgccgacg ccggtggtcg cgccctgata gggctcgatg tagctcggat
     5341 ggttgtggct ctccatcttg aacacgaccg cctggccgtc gccgatgtcg atgacgccgg
     5401 cgttctcgcc cggcccctgg atcacccacg gcgccttggt cggcaacccc ctgagatgca
     5461 gccgcgagga tttatacgag cagtgctcgt tccacatcgc cgagaaaatc ccaagctcgg
     5521 tgaaactcgg cacccggccg atcagtttta gaatgcgctc atattcgtcg ggcttcagcc
     5581 catggctggc gacgagctcg ggggtgattt gcggttggtt catgaggctt cttattaggg
     5641 agatcgggag gggccgaaaa ggccttttat ggcgcaattt cctgctgtcc atagatttgc
     5701 ggacgcccaa tgcatcacgg gggtttgcac atccggcgta gatcggattt aagggctcaa
     5761 atacctcaaa ccaaggccaa atcgcttgga cgacctgacc aaatccgcat tcctcaaccg
     5821 ccccgatctg catgtcgaaa ccgagggcga attcaagggc tggcggacct ggagccgcga
     5881 caacttcgaa tcccataacg ggccgttctg gcaccggatg gaggatgacg gccagatccg
     5941 ctgcgcgttc agggtcgaga agaaacacct caatggcatg agcaatgtcc atggcggcgc
     6001 cttcatggcg ttcgcggact attgcctgtt cgcgatggcc gcgccggtgt tgcaggggcc
     6061 gggggtgacg gtgtcgttcg gctgcgaatt tctcgacgcc gcgcgcgagg gcgaactggt
     6121 cgaaggcacc ggcgacatca tgcgcgccgg cggctcgatg atctttttgc gcggcctgtt
     6181 gaaatcggcc gagcgtccgc tgttcacgtt ttcaggcacc atcaaacgcg taaagcggcg
     6241 aatgccggca gcggcggccc cttccgccgg ttcaggctga cagctgcttt tcggcgatcg
     6301 aaagccattc ggcctgggcc tggcgcaggt atttgggcgc acgtctcatc tgaatcagca
     6361 gttcgttgaa caccaccctg gcctcggcgg tgcgccctac cagattgagc aacagtccgt
     6421 aacgcacccg cgcctccgcg ccggggaagt aggctgcgac cgcatgaaac tcctcgagcg
     6481 cctcatcgac gcgcccgact gcgcccagtg cccgggcata gagcaaatga ccttccgcgg
     6541 attgaaaatc cggccagcgc ttttgcaggt cgtccagcgt cgcgagcgac tcggccggac
     6601 gatcgcgacc gaactgggcc ttcgccttgc ccaacgcata gaccgggtcg tcgcccagcg
     6661 gcaactgcaa gatgtgatcg tagtgtcgtt cggcttctgc gaaacgttcg atcctcagac
     6721 actcggcggc aagcgcaacg cggttggcga tggtgtctgt ggcggcgagc cgatcagata
     6781 gttcacgata caacttttcc ggatcgagcc tattggccac gcggcgacgt gcctgttggg
     6841 cgcctggccc gctcagcatc tccggcacca gttcgaccaa tatgtaagcc aaagcgccga
     6901 ttccgggaat catcaggatg atgaagcccc agggttgcaa gcgccctgtt ctggatgcgt
     6961 gataaaacga gcgtgatatc gagcagcagt atgactaatg cgaacggcac ggcgaattcc
     7021 aagaaagcgg tcgggtcgtc ttgtctgtcg ccatgataac ccggcaggtt cgatcgaaca
     7081 atcccgggtt gacgctcatc gctctgtccc gcacgcccgt ccctgcggat cttccttacc
     7141 cggttttatt gcacgctgac cagcgtcaga tcgacaaacc acgactgcgg cgaaacaaaa
     7201 cccttcacct tcgggctcat cgcgcgtggg ttgagatcgt ggacaatgta aagccaggga
     7261 gggttatcga cgagacgttc atgcgccttg cgataggcgg tcgagatgac tttcggatcg
     7321 gtcgcctctg aaatcgtctt gagggcgtcc tcgaattcgt cgtctttcca ttgctcgaaa
     7381 ttgaagccgt ttggcgagaa attggcggcc aggaaatagc gcgccatcac accggcatcc
     7441 gatgatggcg aactgatatt gagtgccatg gcgcctcgca gattcgggct gtcgggcgtg
     7501 gcacgggccg ccgtcagaag aatctgccat tcgatcacgt cgaattccac ggtcacaccg
     7561 caagcctcct tgaggttttg ctgcaaaaat tcgttcatcg gcaacggcaa catttgcccc
     7621 gaaccggaat tcgagatcaa tgccttgaac gacaatggct ttgccggcgt gtatccggcc
     7681 tcggcgagca gcgccttgcc tttggcggga tcgaatttgt agcggttgac ggggttgcca
     7741 aaatcgggat cgccggtctt gagccagccg gccgagggct cagcggtacc attgagcagg
     7801 gacaccagcc cttcccgatc gatgcaataa ttcagcgcct ggcgaacttt cacgtccttg
     7861 aaggggctgt tggcagcacc gatgttgtag aaccagggcc aaacatgggg atatgagccg
     7921 gtcgtaatgg caaagccggc cgatttcagc gaagcaatgc catcggccgg cggcacttcg
     7981 atccagtcga cctggcccga gcgcaaggcg gccagtcgcg aattcgcctc ggggatcggc
     8041 atcagcacca cattgtcgac tttcgccatc ttggcggggt cccagtactt atcccagcgc
     8101 gccagatcgg cctcctgccg cggcacaatc ctggtgatcc ggaacggccc ggtgccggcg
     8161 gccggaaagg tcgcgacctt ggcccaatcc ccaccggcct tttcgaacga ggcgggcgat
     8221 gtgaacaaaa ggtacaccgc catatagggg aagtaagatg ccggcttggt cgtggtgatg
     8281 gcgacggttg cgtcgtcgat cttccggtag ctgcccatca cgggaacgcg cgcccgcgac
     8341 atcgccgaac tcggcggctc gaattgcggg ctggtgctgt tgaaatagcg gtcgaggttc
     8401 cagatcacgg catcggcgtt gaaatccgtg ccgtcgtgga atttcacgcc gtggcgcaga
     8461 tggaagatcc aggtcttgtt atcctccgga tcctgctccc atttctcggc taacccggga
     8521 cgcaacgccg ccaatcggtc ggtcctggtc agatcccata gcaccaatcc ctcgaagatc
     8581 gggtagccga ggaagcgcat tccctcaaag ccgttgttcg gcagcccggt tgccgtgggg
     8641 atatccgacg ccgtcatggc gatgcgcagc gtggtctcgg ccattaccgg ttggtacgac
     8701 agcaacacgg ctgcgagagc gacggtgcag gaggctttat gaatcagcat gatatccctc
     8761 tgtcctacga tcggacgatc cgctttcagc gaagtgcagg gaattcagga gcaaatttca
     8821 ggccagccgg agaccccgcg cggtcgatgg ccagctggct gcccgctatt gtggcgccgc
     8881 cggcacgatc atgatgatag ggcgttttgg gttaatcctg cgcgcaggcg gcggtgacaa
     8941 agaatgccag cgccgcaacg accgcgggca accgcgcggt tgcatcgttc aggccgcgcg
     9001 ctccagatgc gccaccagcc cggcaaacag gccacgaccg tcggtgcagc ccatgatgtc
     9061 ttcgacgtgg ttttccgggt gcggcatcat gccgagcacg ttgccgcgct cgttgacgat
     9121 gccggcgatc gattgcgccg cgccgttgat gttggagttt tcgtcgacca cgccctcggc
     9181 ggagcaatag cgatagagca cccgcccctc gccttcgagc cgcttcagcg tctgttcgtc
     9241 cgcctcgtaa ttgccctcgc catgcgcgat cggcacccgg atcacctgcc cggcattata
     9301 gccgcgtgtg aacggcgtgt cggagcgttc gacccgcaga tgcacgtcgt ggcagatgaa
     9361 tttcaatcgc gcgttgcgca tgagtacgcc gggcaacagc ccggcctcgc agagaatctg
     9421 gaaaccgttg cagaccccga ccacgagacc gccctggacc gcgaaggcgc gcaccgcgtc
     9481 catgatcggc gcacgcgcgg cgatggcacc gcagcgcaaa taatcgccgt aggaaaatcc
     9541 gccgggcacc accacgaggt cagtacccgc aggcagcgcc gtctcggcgt gccaaaccat
     9601 ctgaggctca tgaccggaga tcagtttcag cgcgcgcgcc atgtcacgct cgcgattgat
     9661 tccgggaaag acgagaacgg cggatttcat gtggctcgct tgcctagctc agaacttcca
     9721 cccggtaatt ctcgatcacg gtgttcgcca gcagcttgtc ggcggcgtcc ttcagcgccg
     9781 cttcggcctt ggcggtgtcg gagccggata gttcgatgtc gaacaccttg ccctgccgca
     9841 cgctggccac gccatcgacg ccgagcgatt tcagcgcccc ttcgatggcc ttgccctgcg
     9901 gatcgaggat gcctgacttc agtgtaaccg tgacgcgcgc tttcactgaa accctcagct
     9961 cttcaccagc accggacccg agccgatcgg ccgctcgttt tccatcagga tgcccaatcg
    10021 cttggcaacc tcggtatagg cctccagcag gccgccgaga tcccggcgga aacggtcctt
    10081 gtcgagcttc tcgttcgatt tgacgtccca cagccggcac gaatcaggcg agatctcgtc
    10141 ggcgacgatg attcgcatca tgtc
//
LOCUS       DATNOI010000113        15242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2273940,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000113 DATNOI010000000
VERSION     DATNOI010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15242
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2273940"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VN968_04055"
     CDS             complement(<1..175)
                     /locus_tag="VN968_04055"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HXL28449.1"
                     /translation="MFEGWDAVVLARAQFAFTMSFHIVFPAFSIGLASYLAVLEALWL
                     WTGREVFINLFNYW"
     gene            complement(564..1130)
                     /locus_tag="VN968_04060"
     CDS             complement(564..1130)
                     /locus_tag="VN968_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXL28450.1"
                     /translation="MTIRSFTATASVLALCLIGAASPGIGDEAARDQTLPFTVAAVQG
                     TAPNFTGISHWFNSAPLNIADLRGKVVLVDFWTYGCVNCVNTLPHVTALYDKYKDRGL
                     VVVGVHTPEFPFERSASNVQAALKRHGIAYPVAQDNDSQTWNAYRNQYWPAQYIVDQS
                     GKIVFQHDGEGRYEEIDRTIARLLNASS"
     gene            complement(1157..1855)
                     /locus_tag="VN968_04065"
     CDS             complement(1157..1855)
                     /locus_tag="VN968_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HXL28451.1"
                     /translation="MVNLVLALLAGVVTVAAPCTLPMLPILLGASVGQTGKIRPAMIA
                     LGFVISFSAAALALSAITRIFDFDPNSLRTGAAILLLGFGLLMIWPAPFEWLSIRISG
                     LTNVGSAAVTSRQGAIGGFVLGTTLGLVWTPCAGPVLGSILTVVATSRDTAWASVLLV
                     VYAIGAALPMLAIAYGGQAVTARVRSIARISPKLQQGFGVVVIGFAIASYFQYDTQIV
                     AWLTAFYPTGQIGL"
     gene            complement(2083..2286)
                     /locus_tag="VN968_04070"
     CDS             complement(2083..2286)
                     /locus_tag="VN968_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28452.1"
                     /translation="MLKPSFRLFIIPLGAICTLASLSSQNVTPRAQRDAGSSFVRERV
                     VDCSNKNPSEVCVISPDSASSAR"
     gene            complement(2463..3074)
                     /locus_tag="VN968_04075"
     CDS             complement(2463..3074)
                     /locus_tag="VN968_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXL28453.1"
                     /translation="MQNVIAINAAARQGIIAAQATSDEMLLESIADGGRPAMHILYSR
                     HNVRVYRFILRIVRDSTMAEDLVSQVFLDVWRTARQFEGRSQVSTWLLSIARFKALTA
                     LRQRKYEDIDQDDVLEIADEADTPEDSLDRSNTSAILRACVAKLSPAHREIINLVYYD
                     EKSVEEVGAIIGIPQSTVKTRMFYARKQLADLLKGAGVDCLAA"
     gene            complement(3436..4176)
                     /locus_tag="VN968_04080"
     CDS             complement(3436..4176)
                     /locus_tag="VN968_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL28454.1"
                     /translation="MTQSQHIIIVDDEAPAREMVGDYLKMHGFTVTLCDGGKSLRAAI
                     ETSVPDLVVLDLNMPEEDGLSIIRDLKSRINVPVIMLTATASPIDRVVGLELGADDYV
                     AKPCELRELMARIRSVLRRSSPSRAAATSEGAGAKAAKERLVRFGTKWLDLEAQALRD
                     DEGNEHPLTASEFGLLKVFAANPKRVLSRERLLELANARDSEAFDRAVDLRIMRIRRK
                     IEPDPSKPAVIRTIRGGGYLFSPAGEKG"
     gene            complement(4173..5918)
                     /locus_tag="VN968_04085"
     CDS             complement(4173..5918)
                     /locus_tag="VN968_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HXL28455.1"
                     /translation="MAEQDDVLHLIDDSGTAPEPPTARKWKVAVIDDDHAVHEGTRFA
                     LSDYVLHGQTLEILSAYSAAEGRALMRAHPDIAAVLLDVIMETDAAGLDLVEYIRNDI
                     KNETVRIILRTGQPGQAPERRVIVQYDINDYKAKTELTADKLFTSLTAALRSYQQLER
                     MVQTRRGLEIIIDAASTLYDFKSMQRLAEGVLTQIASLLNVDCAGILVLRDGGAVGDD
                     FSVLAGSGCYSRFTGVAGSKLLDPDVRAMVEAAFRRRKHEFFDQRSVLYIRTGSGREV
                     VVLLQAERQLSETDRSLVEIFGSRLSIAFDNVILYQQLHEANTQLEDRVAQRTRALMQ
                     ANRRLSSQWLRLQRANGFKNEILGTVAHDLKNPLGVILGRTEMLTELISAGSSKDSVT
                     AQVEHIRDATKRLTLMVDHLISDAMADAFDITIRREPVDIAGLVSEVADANQPLATNK
                     QQAITVSAPPNFVTMCDADRMREAIDNLVSNAIKYSPIGGKIAVAVGRERNNTMIRIT
                     DEGAGLSPEDLGRLFGRFQRLSAKPTAGESSTGLGLSIVKRIIDMHGGQVTAESAGPG
                     QGSTFTVTLPATELS"
     gene            complement(5888..7939)
                     /locus_tag="VN968_04090"
     CDS             complement(5888..7939)
                     /locus_tag="VN968_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL28456.1"
                     /translation="MPAGLAARGRSRIVGLVRAVPIRWRILSIAALNSAVVVVLAAMI
                     WNGAQVLGSAWDDVRQVRESDKILALLESETGRLQNLIHRYINQPSPELFAEILLLRE
                     AVLGTLTTRASTDPMLSGSVKDLERVTERFLNGFGELRGVQATISKTYEDQVLAPARD
                     MAGLYSIIEGATGHRDALIWPSLGKSREAFTAMLVAANAYYLSLASASAEDARRNTET
                     IEKTIPVMSDLADNDLQRMALQRLRMRAVALREGLAKLSDQLASRTELLRNTIDASQA
                     EAIGAIDELSIKMRQREQKAQETFDRTLGDISRRVLSIAVIFLGIIITAGVVIALTIR
                     LPLQQIMAAMHAITTGNYDRQVQGTGAKDEVGAMARAVEVFRENAIAKRQTEDELRTS
                     KEKAEGALFELNAAQKNLIDAERLAALGGLVAGVAHEVNNPIGISLTVASSFARRAEM
                     FDAELRSDGQLRRSQLEEFVRTSRDASQQLVSNLHRAGELIQSFKQVAVDRSHAERRQ
                     FSLSEATDQIIASLRPVLKRAPIALSVDVPEGLVIDGYPGSYGQILTNLFLNAVNHAF
                     ADKRSGAISISARPRGHDDVEIIFADNGAGMTPDVQRQAFDPFFTTRRNEGGTGLGLH
                     IVYNLVTQQLGGRMMLDSRLGQGTTFRIIMPKAAKGGSATADPASDGTPQWPNRTMSS
                     T"
     gene            complement(7980..8984)
                     /locus_tag="VN968_04095"
     CDS             complement(7980..8984)
                     /locus_tag="VN968_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXL28457.1"
                     /translation="MLVYSRADLSRTVTIVAIGVALCLAAVSTTAVAREFRVADTQSE
                     DYPTVQALRYMGRLIEERSGGRHHLRVFHSRQLGEEKETIEQTRVGAIDLNRTNVALV
                     GTFVPAMNVLAMPFLFRSIDHLQKVLDGPIGDELLGSFEAHGFVGLAFYDSGARSIYN
                     SVRPIRSIADVKGLRLRVQQSELMSDMIKALGAEPIELPYGQVLTGLATRLIDGAENN
                     WPSFVTTDHYKYAGYYTLTEHTMSPEVLLMSQKAWASLSSEDRKIFREAAMRSSQFMR
                     EKWKDLEDQSRERAKAAGVTVVTDFDRKPFEAAMAAIYAKAQRDSASAKLIERIRKVE
                     "
     gene            9391..10671
                     /gene="ugpB"
                     /locus_tag="VN968_04100"
     CDS             9391..10671
                     /gene="ugpB"
                     /locus_tag="VN968_04100"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HXL28458.1"
                     /translation="MVAFASPAQAVTDIMWWHAMSGELGRQLEKLAADFNASQSEYRI
                     VPHYKGNYTETVNAAIFAFRSRGQPAIVQVNEIATATMMAARGAIYPVFELMRDEQEP
                     FSPDAYLPAVTGYYADVAGNMLSFPFNASTPILYYNKNLFRSAGLDPETAPKTWPEVG
                     AAAKKLRASGAVCGFTTSWPSWINVENFSAFHNLAISSKANGFEGLDAVLIFNNPVMV
                     RHIAQLAEWQTGKAFDYSGRDTTAEPRFQNGECGIFIGSSATRADIKANSKFEVGYGM
                     LPFWPDVEGAPQNSIIGGATLWVLRDRPRAEYKGVAKFFGFLSKPEVQAAWHQNTGYL
                     PITRAAFDLTRAQGFYDRNPGAAISIEQITLKPPTDNSRGVRLGSFVLIRSVIDEELE
                     QAFAGKKSAQQALDSAVEHGNRLLRQFERANPDR"
     gene            10905..12524
                     /locus_tag="VN968_04105"
     CDS             10905..12524
                     /locus_tag="VN968_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL28459.1"
                     /translation="MKRRDFLKSVSGLAAGAMVPAPAIWSAAKADARSETLLIVSEGG
                     PNNLDIHGVGTNVPGYEVSWNCYDRLIGHEMKSGPGGVPYYDRDKFKPELAEDMNVGD
                     MSVTFKLKKNAKFHDGAPVTARDVKWSLDRAVSVGGFPTFQMSAGSLTKPEQFVIVDD
                     NTVRVDFARKDRLTIPDLAVIVPCVVNSELVKKNASEKDPWGLEYTKQQTAGSGAYRV
                     TKWTAGTEVIMERNDDWAGGPLPKIKRVIWRMVPQAGNRRALLERGDADISYELPYKD
                     FQELKSAGKLDIVSLPFSNGIQYIGMNVTKPPFDNPKVRQAVAYAIPYQKIMDAVLFG
                     LANPMFGAAPTKTTDVAWPQPTKFFTDIDKAKALLAEAGYPNGFETTLSFDLNFAGVN
                     EPLCVLVQESLGQIGIKTTINKVPGANWRTELTKKELPLYTNVFSGWLDYPEYFFYWC
                     YHGNNSIFNTMSYKSAEMDRLIDGARNAASTGDKPTYDADVKGFVDLAFADIPRIPLY
                     QPYVNVAMQKNVSGYQYWFHRRLDYRALVKA"
     gene            12753..13766
                     /locus_tag="VN968_04110"
     CDS             12753..13766
                     /locus_tag="VN968_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL28460.1"
                     /translation="MLTMIGKRLMFAIPSLIGVVIVTFLLTRALPGDPAAYFAGPAAT
                     KEAVEQIRKKLGFDKPLIEQFFRYTADLAHGDFGNSLTTGQPVATEIRNRLPASAELT
                     LLGLVISVAIALPLGVLAATRPGSWIDHLCRVTTTAGVSLPVFFTGLVLVYVFYFRLG
                     WSPAPLGRLDVFYSAPPSVTGFYLIDALIARDFETFRSAFSQLILPALTLAIFSLAPI
                     ARMTRASMLAVLSSDFVRTARASGLSPGTVIVTYAFRNAMLPVITTLSMVFSFLLGAN
                     VLVEKVFAWPGIGSYAVEALISSDFAPVQGFVLTMAVLYVLLNLMIDILYGVIDPRVR
                     LEG"
     gene            13788..14687
                     /locus_tag="VN968_04115"
     CDS             13788..14687
                     /locus_tag="VN968_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL28461.1"
                     /translation="MSSVAPAVEPVAPARTSGLSAIFEHTRYVLGENRVTAFAFALLI
                     LIVFAALFGPYVVPHDPLASDTAAALKPPSSAHWFGTDQLGRDIFSRVIVATRLDTFI
                     AVASVTLVFLMGGLAGIAAGYFGGWTDRIVGRVADTIMAFPLFVLAMGIVAALGNTVQ
                     NIIIATAIVNFPLYARVARAEANVRRDAGFVQAARLSGNSEFRILLVHILPNIMPIMI
                     VQMSLTMGYAILNAAGLSFIGLGVRPPTAEWGIMVAEGAGFMVSGEWWIALFPGLALM
                     VAVFCFNLLGDGLRDIVDPQRRT"
     gene            14897..>15242
                     /locus_tag="VN968_04120"
     CDS             14897..>15242
                     /locus_tag="VN968_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL28462.1"
                     /translation="MTAPPLLDVRDLTVEFATRRGIVRAVQHVNISIAKGETLGVVGE
                     SGSGKSVTSYAVMRILDRAGRIADGSVVFSGIDVGSASEDQMRDLRGREISMIFQNPR
                     AALNPIRKVGDQI"
BASE COUNT         2785 a         4658 c         4809 g         2990 t
ORIGIN      
        1 gccagtagtt gaacaggttg atgaagacct cgcggccggt ccatagccag agcgcttcga
       61 gcaccgcgag atagcttgca agcccgatcg agaaagcggg aaacacgatg tgaaacgaca
      121 tcgtgaatgc gaactgcgcc cgggccaaca ccacggcatc ccagccctcg aacatcggca
      181 ccatccattg ctgtcagtga gcgcttctac cacgctcgtc gttgtgatgg aattgttgcg
      241 gcatcgaagc atcgaagtgg atcaatcgcc ccacccgagc cttaagcgcg agccccgctc
      301 gcgtcgacca cgaggggaga ggcgagccga gagtcggccg gagaagtagg acgttcatca
      361 acggaagcta gggcgatgtg ttattcagcc ggcgcttgcg cgtcacgtgt tcgtgaggct
      421 gctcaacgca gcagaaggca ggccgaaaaa ggcagggtga cccgaagggg gcatcccctc
      481 cggatcgtcg caggtgtttg gaaatcagaa ataaatctcg ggcgctggtc ttgctgttgc
      541 agctcgatag cgcagggtgc tgctcagctg gatgcgttga gcaggcgcgc aatggtgcgg
      601 tcgatctctt cgtaccggcc ttcgccgtcg tgctggaaca cgatttttcc gctctggtcg
      661 acgatgtatt gcgcgggcca atactggttg cgataggcgt tccaggtttg cgaatcgttg
      721 tcctgcgcca ccggataggc gatgccgtgc cgtttcagcg cggcctgcac gttggatgcg
      781 gatcgctcga acgggaattc cggcgtgtgg acgccgacca cgaccaggcc gcggtccttg
      841 tatttgtcat aaagcgcggt gacgtggggc agcgtgttga cgcagttgac gcagccatag
      901 gtccagaaat ccaccagcac caccttgccg cggaggtcgg cgatgttgag cggggcggaa
      961 ttgaaccagt ggctgatgcc ggtgaagttc ggcgccgtac cctgcaccgc ggcgacagtg
     1021 aacggaagtg tctggtcgcg cgccgcttca tcgccgatgc cgggagacgc tgcgccgatc
     1081 aggcacaggg caagcacgga agcggttgcg gtgaacgatc gtatcgtcat ggaagtctcc
     1141 tgtggtcgcg ggttgattac aggccgatct ggccggtcgg gtagaatgcg gtcagccacg
     1201 ccacgatctg cgtgtcgtat tgaaaatagg atgcgatcgc gaagccgatc accacgacgc
     1261 caaatccctg ctgcagtttt ggggagatgc gcgcgatgct gcgcacgcgg gcggtgacgg
     1321 cctggccgcc ataggcgatc gccagcatcg gaagcgcagc gccgatggca tacaccacga
     1381 gcaacacgct cgcccatgcg gtatcccgcg atgtcgcgac cacggtgagg attgaaccga
     1441 gcacggggcc ggcgcagggt gtccagacca gaccgagcgt ggtgccgagc acgaagccgc
     1501 cgatggcgcc ttggcgggac gtgacggcgg cggagccgac attcgtcagc ccgctgatgc
     1561 ggatcgacag ccactcgaac ggcgccggcc agatcatcaa gaggccaaac ccgagcaaca
     1621 gaatcgccgc gccggtccgc aggctattgg ggtcgaagtc gaagatgcgg gtgatcgcgc
     1681 tcagagccaa tgcggcggcg gaaaacgaga tgacgaaacc aagcgcgatc atcgccggcc
     1741 gtatcttgcc ggtttggcca accgatgcgc caagcaggat gggcagcatt ggcagcgtac
     1801 agggcgccgc cacggtgacc acgccggcga gcaatgcaag aaccaggttg accatcaggg
     1861 aaggtccatg gaaccacggg cttcgcggcc aggcgcgagc tctcgatcgt tcttcgcggc
     1921 actaggacga ggcgttacct tgggcccgcc ccagccccgt cacgtgttcg tgaggctttc
     1981 ggcggccgcc agaagcagag cgacccggag gggggcatcc cgtccgggtc gttggagtta
     2041 cttggcaggt tagaaggcct ggtcggaaac gaaaaacctg ggtcagcggg cgctgctcgc
     2101 gctgtcgggg gagatcacgc agacctccga cggattcttg ttgctgcaat cgaccacccg
     2161 ctcgcgcacg aagcttgagc cggcatcgcg ttgcgcgcgg ggcgttacgt tctggctcga
     2221 cagcgaggcc agggtgcaga tggcgccgag cggaatgatg aagaggcgga aagacggctt
     2281 cagcattttt tctcactcct gttcggctgc gttgctgatg gtctgtatag gcagagagtt
     2341 tttccgcttg atgtcgccaa ttgtttcaat tgtttcgccg gaattaatat tccggcagaa
     2401 aattgtgcca gttcttgtgt ccgcaatacc cgtaacccag ttgaaatagc tgacgtttta
     2461 cctcaggccg ccaggcagtc cacgccggct cccttgagca gatcggcgag ctgcttgcgg
     2521 gcatagaaca tccgggtctt caccgtgctc tgggggatgc cgatgatcgc gccgacctct
     2581 tccaccgact tctcatcgta gtagacgagg ttgatgatct cgcgatgcgc cggcgatagt
     2641 ttcgcgacgc acgcgcgcag aatggcgctg gtgttgctgc ggtcaagcga gtcttcgggc
     2701 gtgtcggcct cgtcggcgat ttccagcacg tcgtcctggt cgatgtcctc gtacttgcgc
     2761 tgacgcagcg cggtcagcgc cttgaagcgc gcgatcgaca acagccaggt ggaaacctgg
     2821 gaacggcctt cgaactgacg cgcggtgcgc cacacatcca ggaagacctg gctgacaagg
     2881 tcttcggcca tcgtcgaatc gcgcacgatc cggaggatga agcggtacac ccgcacattg
     2941 tgacgggagt aaaggatatg catcgcgggc cggccgccat cggcaatgct ttccagcagc
     3001 atttcatcgg aggtcgcctg ggcagcgatg atgccttggc gggctgcggc gttgatggcg
     3061 atgacgttct gcatgactgg ctcccggtga ttgcgctttt gcgctttgtt gggcggattg
     3121 atagccagcg aacgtttcgg gatgtctgca cgccaacggg aaaatggttt caaacgtcag
     3181 agaattgttt cgtcgcggcg cccgggacga aacattcggg cccgaaatgg gagtaatttc
     3241 aatgcgcgca aaattggaag cttgctgaaa ggcgctgatc tatccgttat ggccgggctt
     3301 cgtcccggcc atccgcgtct ttcttttctg actgcggcaa agacctggat gcccggcaca
     3361 aggcgcacaa ggccgggcat gacggtgcat cacgacaaga aacgttacgc gcaggtagcc
     3421 cgtgatttcg gccaatcagc ccttttcgcc ggcgggcgaa aacagatatc cgccgcctcg
     3481 gatggtgcgg atcaccgcgg gcttgctcgg gtccggttcg atcttgcgcc ggattcgcat
     3541 gatccgcaga tcgaccgcgc gatcgaaggc ctcgctgtcg cgcgcgttgg ccagttccag
     3601 caggcgctcg cgcgagagca cgcgcttcgg attggcggca aaaaccttga gcaggccgaa
     3661 ttcggaagcg gtcagcggat gttcattgcc ctcgtcgtcg cgcagcgcct gcgcctcgag
     3721 atcgagccat ttcgttccga accgcaccag gcgctctttc gccgctttcg ctcccgcgcc
     3781 ctccgaggtt gccgccgccc ttgacgggga gctgcgccgc aacaccgagc ggatccgcgc
     3841 catcagttcg cgcaactcgc agggctttgc gacgtaatcg tcggcgccga gttccagtcc
     3901 gaccacgcgg tcgatcgggc tggcggtcgc cgtcagcatg atgaccggga cattgatgcg
     3961 gctcttcaga tcgcggatga tcgaaagccc gtcttcctcc ggcatattga gatcgagcac
     4021 caccagatcg ggcacgcttg tctcgatcgc cgcgcgcaag ctcttgccgc cgtcgcatag
     4081 cgtcacggta aagccgtgca tcttcagata atcgccgacc atctcgcggg ccggcgcctc
     4141 gtcatcgacg atgatgatgt gctggctttg ggtcatgaca actctgtcgc cggcaaggtg
     4201 acggtgaagg tcgatccctg cccgggaccg gcgctttcgg cggtcacctg gccgccatgc
     4261 atgtcgataa tgcgcttgac gatggaaagc ccgaggccgg tcgagctttc gccggcggtg
     4321 ggcttggccg atagccgctg gaagcggccg aacaaccggc cgagatcctc gggcgacagt
     4381 cctgcgcctt cgtcggtgat ccggatcatg gtgttgttcc gctcgcgacc gaccgccacc
     4441 gcgatcttgc cgccgatcgg gctgtatttg atggcgttgc tgacgagatt gtcgatcgcc
     4501 tcccgcatcc ggtcggcgtc gcacatggtg acgaaattcg gcggcgccga caccgtgatg
     4561 gcctgctgct tgtttgtagc cagcggctgg ttggcatctg caacttcgct gaccaggcct
     4621 gcgatgtcga ccggctcgcg gcgaatggtg atgtcgaagg catccgccat cgcatccgag
     4681 atcagatgat cgaccatcag ggtcaggcgc ttggtggcat cgcggatgtg ttcgacctgg
     4741 gcggtgacgc tgtccttgga agagccggcg ctgatcagtt ccgtcagcat ttcagtgcgg
     4801 ccgaggatca ccccgagtgg attcttcaga tcgtgggcca cggtgccgag aatttcgttc
     4861 ttgaagccgt tggcgcgctg tagtcgcagc cattgcgacg aaagcctgcg gttggcctgc
     4921 atcagggcac gggtgcgctg ggcgacgcgg tcctcgagct gggtattggc ctcgtgcagc
     4981 tgctgataaa gaatgacgtt gtcgaacgcg atcgaaagcc ggctgccgaa aatctcgacc
     5041 agcgatcgat cggtttcgga tagctggcgc tcggcctgca acagcacgac gacttcccgt
     5101 ccgctgccgg tgcgaatgta gagaaccgag cgctgatcga agaactcatg cttgcgccgc
     5161 ctgaaggcgg cttccaccat cgcgcgcacg tcggggtcga gcaatttgga gccggcgacg
     5221 ccggtgaagc ggctatagca gccagaaccg gccagcaccg aaaagtcgtc gccgaccgcg
     5281 ccgccgtcgc gcagcaccag gatgccggcg caatcgacgt tgagaagcga agcgatttgc
     5341 gtcagcacgc cctcggccag acgctgcatc gatttgaagt catacagcgt cgaggcggcg
     5401 tcgatgatga tttccagtcc gcgccgggtc tgaaccatgc gctcgagctg ctgatagctg
     5461 cgcaacgccg cggtcagcga ggtaaacaac ttgtccgcgg tcagctcggt tttcgccttg
     5521 tagtcgttga tgtcgtattg gacgatgacg cggcgctcgg gcgcctggcc gggttgcccg
     5581 gtgcgcagga taatccgcac cgtctcgttc ttgatgtcat tgcggatgta ttcgacgaga
     5641 tcgaggcccg cggcgtcggt ctccatgatg acgtcgagca gcacggcggc gatgtcgggg
     5701 tgcgcgcgca tcaaggcccg gccctcggcc gccgaatagg ccgacaggat ttccagggtc
     5761 tggccatgga gaacgtagtc gctcagcgca aaccgcgtac cttcatggac ggcgtgatcg
     5821 tcgtcgatca cggcgacctt ccatttgcgc gcggtcggcg gttccggagc ggttccggaa
     5881 tcgtcgatca ggtggaggac atcgtcctgt tcggccattg aggggttccg tcgcttgctg
     5941 ggtctgcggt ggcggagccg cccttggcgg ccttcggcat aataatgcga aatgtggtgc
     6001 cttgtcccag ccttgaatcc agcatcatgc ggccgccgag ctgctgggta accaggttgt
     6061 aaacgatgtg caggccgagc ccggtgccgc cctcgttgcg gcgtgtggta aagaacgggt
     6121 cgaacgcctg ccgctgcacg tccggcgtca ttccggcacc attgtcggca aaaatgatct
     6181 cgacgtcgtc atggccgcgg ggcctcgcgg aaatcgagat cgccccggaa cgcttgtcgg
     6241 caaatgcatg atttaccgcg ttgaggaaca gattggtcag aatctggcca tacgagccgg
     6301 gatagccgtc gatcacaagg ccttccggca cgtcgaccga tagcgcgatc ggcgccctct
     6361 tcagaaccgg ccgcaagctg gcgatgatct ggtcggtcgc ctcgctcagg ctgaattgcc
     6421 ggcgctccgc atgcgagcgg tcgaccgcga cttgcttgaa cgactggatc aattcgccgg
     6481 cgcgatgcag gttcgacacc agttgctggg atgcgtcgcg cgaggtgcgg acaaattcct
     6541 cgagttggga ccggcgcaac tggccgtcgg atctcagctc ggcgtcgaac atctcggcgc
     6601 ggcgggcgaa actcgacgcc accgtcaggc tgatgccgat cgggttgttc acttcatggg
     6661 caacgccggc gaccagaccg ccgagcgcgg ccagacgttc ggcgtcgatc aggtttttct
     6721 gggcggcgtt caactcaaac agcgcgccct cagctttctc tttcgaggtg cgcaactcat
     6781 cctcggtctg gcgcttggcg atggcatttt cgcggaacac ctccaccgcg cgggccatcg
     6841 cgccgacctc gtccttggcg ccggttccct gcacctgccg atcgtaattg cccgttgtga
     6901 tggcatgcat cgccgccatg atctgctgca acggcaggcg gatggtcaac gcgatcacga
     6961 cgcccgcggt gatgatgatg ccgagaaaaa tcacggcaat ggacagcacc cggcgtgata
     7021 tatcgccgag cgtccgatcg aatgtctcct gcgctttctg ttcgcgctgc cgcatcttga
     7081 tcgacaattc gtcgatggcg ccgatggctt cggcctggct ggcgtcgatg gtattgcgca
     7141 acagctcggt ccggctggcg agctggtccg acaattttgc gaggccctcg cgcagcgcca
     7201 ccgcgcgcat gcgcaatcgt tgtagcgcca tgcgctgcag gtcgttgtcg gcgaggtcgg
     7261 acattaccgg gatcgtcttc tcgatcgtct cggtattgcg gcgggcgtcc tcggcggatg
     7321 ccgaggccag cgaaagataa taggcgttgg cggcaaccag catcgcggta aacgcctcgc
     7381 gggatttgcc aagcgacggc cagatcaggg catcgcggtg cccggtcgcg ccctcgatga
     7441 tcgagtagag cccggccatg tcacgggcag gggccagcac ctggtcctca taggttttcg
     7501 aaattgttgc ctggacgccg cgcagttcgc cgaagccgtt gaggaaacgc tcggtgacgc
     7561 gttcgaggtc cttcaccgag cccgacagca tcgggtcggt cgatgcgcgc gtggtcaggg
     7621 tgccgagcac cgcctcgcgc aacagcagga tctcggcgaa cagttcgggg cttggctggt
     7681 tgatgtagcg atggatcagg ttttgcaggc ggccggtctc gctctccaat aacgccagga
     7741 ttttgtccga ttcccgcacc tgccgcacat cgtcccaggc cgaaccgagc acctgggcgc
     7801 cgttccatat catggcggca agcaccacga cgacggcgga gttcagcgcg gcaatcgaca
     7861 ggatgcgcca gcggatcggc accgcgcgga ccagcccgac gatgcgggaa cggccgcgcg
     7921 cggcgaggcc cgccggcagt tccgcagcac catcctgttg gggtcttccc agggccaatt
     7981 cactccacct tgcgaattcg ttcgatcagt ttggcggatg ccgaatcgcg ttgcgcttta
     8041 gcgtagattg ccgccatcgc ggcttcgaaa ggtttgcggt cgaagtcggt cacgaccgtc
     8101 acgccggccg ctttcgcccg ctcgcgcgac tggtcctcga ggtccttcca tttttcgcgc
     8161 atgaactggc tggatcgcat cgctgcctcg cgaaaaatct ttcggtcctc gctcgacagg
     8221 ctcgcccagg ccttctggga catcagcagc acttccgggc tcatggtgtg ttcggtcagc
     8281 gtataataac cggcatattt gtagtgatcg gtagtgacaa aggaaggcca gttattctcc
     8341 gcgccgtcga tcagcctggt ggcaagtccg gtaagaactt gcccataggg caactcgatc
     8401 ggctccgcgc caagcgcctt gatcatgtcg gacatcaatt ccgattgctg cacgcgaagt
     8461 cgcaagccct tgacgtcggc gatagagcgg atcggcctga cgctgttgta gatcgaccgt
     8521 gccccggaat cgtagaacgc caggccaaca aatccgtgag cctcgaagct gccaagtaat
     8581 tcatcaccga tcgggccgtc cagcaccttc tgcaaatgat cgatggaacg aaacaaaaac
     8641 ggcatcgcga gcacattcat tgccggcacg aaagttccga ccagcgccac attggtacga
     8701 ttgaggtcga tggcgccgac gcgggtctgt tcaatggttt ccttttcctc gccgagctgg
     8761 cgcgaatgaa agacccggag gtggtggcgt cctccgctgc gctcctcgat caggcggccc
     8821 atgtatcgca gcgcctgtac cgttggatag tcttcgcttt gggtgtcggc gacgcgaaat
     8881 tcgcgcgcca cggcggtcgt cgagaccgcc gccaaacaaa gcgcgacacc gatggctacg
     8941 attgttacgg tccgcgaaag gtcggcgcgg ctgtacacga gcaactatcc cctcatgatg
     9001 atgtcgatgg tcgggaaaaa aggttcaacg ctttctagca gaagccggcg ggagatagct
     9061 attctgccag caccggtgac gtcgtcaatg gtgcgctgcg acggccgttg aaaccactgg
     9121 cgtgggccgc ccactgcaag ggctctttga ggcggccggc atgcccatcg tcggatccac
     9181 aaccagcagc cgttatggct gtaaaagtga ggtgccgcag gtgcctgcct caaatcgcgc
     9241 cttcacggag tatcggtccc gctttcgcgg ggacgacgtt tatgcttaat caggcgcgca
     9301 acgtgagagt cgtgttctgg ccgcggcaat aagattggtt ttatcgtgaa tccagctttt
     9361 aaatttttgc gattcgctgc cgcggcggcg atggtcgcct tcgcctctcc ggcccaggcg
     9421 gtcaccgata tcatgtggtg gcacgccatg tcgggcgagc ttggccggca gctggagaag
     9481 cttgccgctg acttcaacgc atcgcaatcc gaatatcgga tcgtgccgca ctacaagggg
     9541 aactataccg aaaccgtgaa tgcggcgatc ttcgcgttcc gctcgcgcgg ccagccggcg
     9601 atcgttcagg tcaacgaaat cgcgaccgcg accatgatgg ccgccagggg cgcgatctat
     9661 ccggtgttcg aattgatgcg cgatgagcag gagccatttt caccggacgc gtatcttccg
     9721 gccgtgaccg gctactatgc cgatgtcgcc ggcaacatgc tgtcattccc gttcaacgcc
     9781 tcgaccccga tcctttacta taacaagaac ctgttccgta gcgccgggct cgatcccgaa
     9841 acggccccga agacctggcc ggaggtgggc gcggcggcga agaaattgcg cgcgtcggga
     9901 gccgtttgcg ggttcaccac gtcgtggcca tcatggatca acgtcgagaa cttttccgca
     9961 ttccacaatc tggcgatttc gagcaaggcc aatggcttcg agggtcttga cgcggtgctg
    10021 attttcaaca acccggtgat ggtgcgccac atcgcgcaac ttgcggaatg gcaaaccggc
    10081 aaggcgttcg actacagcgg tcgcgacacc acggcggaac cgcggtttca gaatggcgaa
    10141 tgtggaatct tcatcggctc gtcggcgact cgcgccgata tcaaggccaa ttcgaaattc
    10201 gaggttggct acggcatgtt gccgttctgg cccgacgtcg aaggcgcgcc gcaaaactcc
    10261 attatcggcg gcgcgacatt gtgggtgctg cgggaccggc cgcgcgccga atacaagggg
    10321 gtcgcgaaat tcttcggctt cctgtcaaag ccggaggttc aagccgcgtg gcatcagaac
    10381 accggctacc tgccgatcac ccgcgcggcc ttcgatttaa cccgcgccca gggcttttac
    10441 gaccgcaatc caggtgcggc gatctcgatc gagcagatca cgctaaaacc gccgaccgac
    10501 aattcgaggg gtgtgcggct cggatcgttc gttctgatcc gcagcgtcat cgacgaagaa
    10561 ctggagcagg cttttgccgg taaaaaatcg gcgcagcagg cgctcgattc cgcggtcgag
    10621 cacggcaacc gcctgctgcg tcaattcgag cgggctaacc cggaccggta gcagtctctg
    10681 tttggcgacc tcgcccgaga agaaggcggt cacaatattt gcactcacaa taggcaaagg
    10741 cgcctctttg tatgcaatcc attcggagtc gcgtttcctc cgcacgttct tgatttagct
    10801 gtcaaatcat gcgcttaggg cgccattagg catgcgttaa gatttggcac gaaccttgcg
    10861 aagtcagctc cagacctgcc cacacgtagc gtttggagca tcacatgaag cgtcgcgatt
    10921 tcctgaaatc cgtctccgga ttggccgccg gcgccatggt gccggcgccc gcaatctggt
    10981 ccgccgccaa ggcggatgcc cgctcggaga cgttgctgat cgtctcggaa ggcggcccca
    11041 acaatctcga cattcacggc gtcggcacca acgtgcccgg ttatgaagtg tcgtggaatt
    11101 gctacgaccg cctgatcggt cacgagatga aaagcggtcc cggcggcgtt ccgtattacg
    11161 accgcgacaa gttcaagccg gaactcgccg aagacatgaa tgtcggcgac atgtcggtca
    11221 ccttcaagct gaagaagaac gcaaaattcc acgacggagc gccggtgacg gccagggatg
    11281 tcaaatggtc gctggaccgt gccgtcagcg tcggcggatt cccgaccttc cagatgagcg
    11341 cgggctcgct gacaaaaccc gagcagttcg tcatcgtcga cgacaacacg gtgcgggtgg
    11401 atttcgccag aaaggatcgc ctcacgatcc ccgatctcgc ggtgatcgtg ccctgcgtgg
    11461 tcaattcgga actggtgaag aagaacgctt ccgagaaaga cccctggggc ctcgaatata
    11521 ccaaacagca gaccgcaggc tccggcgcct acagagtgac gaaatggacc gccggcaccg
    11581 aagttatcat ggaacgcaac gacgactggg ccggcggacc gttgccgaag atcaagcgcg
    11641 tgatctggcg catggtgccg caggccggca accgccgcgc gctgctcgag cgcggtgacg
    11701 ccgacatctc ctatgaactg ccgtacaagg attttcagga gctcaaatcg gccggcaagc
    11761 tcgacatcgt gtcgctgccc ttctccaacg gcatccagta catcggcatg aacgtgacca
    11821 aaccgccgtt cgacaatccg aaggtgcgcc aggcggtggc ctacgcgatc ccctatcaga
    11881 agatcatgga tgcggtgctg tttgggctgg ccaatccgat gttcggcgcg gcaccgacca
    11941 agaccaccga cgtcgcctgg ccgcagccga ccaagttttt taccgacatc gacaaggcca
    12001 aggcgctgct tgccgaggcc ggctatccga acggcttcga gaccacgctt tcattcgacc
    12061 tcaacttcgc cggcgtcaac gagccgctct gcgtgctggt gcaggaaagc ctcggccaga
    12121 tcggcatcaa gaccacgatc aacaaggtgc ccggcgccaa ctggcgcacc gaattgacca
    12181 agaaggaact gccgctctac accaacgtgt tctcgggttg gctcgattat ccggaatact
    12241 tcttctattg gtgctatcac ggcaacaatt ccatcttcaa caccatgagc tacaagtcgg
    12301 cggagatgga cagactgatc gacggcgcgc gcaatgcggc ctcaaccggc gacaagccga
    12361 cctacgacgc cgacgtgaaa ggctttgtcg acctggcatt cgccgatatt ccgcgcattc
    12421 cgctctatca gccctatgtc aacgtcgcga tgcagaagaa cgtctcgggc tatcaatact
    12481 ggttccaccg ccgcctcgac taccgcgcgc tggtgaaggc gtaacgcggt gccgagtggt
    12541 cgcgacgcac cggtgcgtgt tgctctctgg ccctttggtg aggagagccg aactccgtca
    12601 ttccctcccc ccttgcgggg gaggggcagg gaggggggta gccacgcgca ccatcttctc
    12661 ggcttacccc tctccccagc cctcccccac aaggggggag ggagcagatc acgccgtgtt
    12721 gcgagagctg aaatccagca cgcgagtgcc ccatgctgac catgatcggc aagcggctga
    12781 tgttcgcgat tccgagtctg atcggggtgg tgatcgtcac gttcctgttg acccgcgcgc
    12841 tgcccggcga tcccgcggcg tatttcgcgg gacctgcggc gaccaaggag gcggtcgagc
    12901 aaatccgcaa gaaactcggc ttcgacaagc cgctgatcga gcagttcttc cgctatacgg
    12961 ccgatctcgc gcatggcgat ttcggcaatt cgctcaccac cggccagcct gtcgcaactg
    13021 aaatccgcaa ccgcctgccg gcctcggccg aactgacgct gctcggcctc gtcatctcgg
    13081 ttgcgatcgc gctgccgctc ggcgtgctcg ccgccacccg tcccggctcg tggatcgacc
    13141 acctctgccg cgtgacgacg accgcgggcg tgtcgctgcc ggtgttcttc accggcctgg
    13201 tgctggtcta tgttttctat ttccgcctcg gatggtcgcc ggcgccgctc ggacggctgg
    13261 atgtgtttta tagcgcgccg ccttcggtaa ccggcttcta tctgatcgat gcgctgatcg
    13321 cgcgcgattt cgaaaccttc cgctccgcat tcagccagtt gatcctgccg gcgctgacgc
    13381 tcgccatctt ctcgctggcg ccgatcgcgc gcatgacgcg ggcctcgatg ctggcggtgc
    13441 tgtcgtcgga tttcgtgcga accgcgcgcg ccagcggtct ttcgccaggt accgtgatcg
    13501 tcacctacgc cttccgcaac gcgatgctgc cggtgatcac cacgctgagc atggtgttct
    13561 cgtttttgct cggcgccaac gtgctggtgg aaaaagtgtt cgcctggccc ggcatcggct
    13621 cctacgcggt ggaagcgctg atctcgtcgg atttcgcgcc ggtgcagggt tttgtgctga
    13681 ccatggcggt gttgtacgtg ctcttgaacc tgatgatcga cattctctat ggcgtgatcg
    13741 atccaagggt aagacttgaa gggtaaggct tgaagggtga ggcaagcatg agcagcgttg
    13801 cgccagctgt tgaacctgtc gcacccgcgc gaacctcggg gctgtcagcg atcttcgagc
    13861 acacccgcta cgtgctcggc gagaaccggg tgaccgcgtt cgccttcgcg ctgttgatcc
    13921 tcattgtgtt tgcggcattg ttcggtcctt atgtcgttcc ccatgatcca ctcgcctccg
    13981 acaccgcggc ggcgctgaag ccgccgtctt ccgcgcactg gttcggcacc gaccagctgg
    14041 ggcgcgatat tttcagccgc gtcatcgtgg cgacccggct cgacactttc atcgcggtgg
    14101 cctcggtgac gctggtgttt ctgatgggcg gtctcgcagg tatcgccgcg ggctattttg
    14161 gcggctggac cgaccgcatc gtcggacgcg tcgccgatac catcatggcg tttccgctgt
    14221 tcgtgctggc gatggggatc gtggcggcgc tcggcaatac cgtgcagaac atcatcatcg
    14281 cgaccgcgat cgtgaatttc ccgctctatg cccgcgtcgc ccgtgccgaa gccaatgtgc
    14341 gccgcgacgc cggcttcgtg caggcggcgc gcctgtcggg caatagcgag tttcgcattt
    14401 tgctggtgca catcctgccg aacatcatgc cgatcatgat cgtgcagatg tcgctgacca
    14461 tgggctacgc catcctcaac gccgccgggc tgtccttcat cgggctcggc gtgcggccgc
    14521 ccacggccga atggggcatc atggtcgcgg aaggcgcggg cttcatggtc tcgggcgaat
    14581 ggtggatcgc gctgtttccg ggtctggcgc tgatggttgc ggtgttctgc ttcaacctgc
    14641 tcggcgacgg cctgcgcgac atcgtcgatc cgcagcgaag gacgtagagg atggcaaagg
    14701 tgtgtggccg cggtctcctt cacctcgccc cgcttgcggg gagaggtcgg tttcgcgcat
    14761 tagcgcgaaa ccgggtgagg gggaccctcc gcgcattcct ctttctcctg cgttcgctga
    14821 tagaagcccc tcaccccaac cctctccccg ccagagcggg gcgagggggc ctgtctcgat
    14881 tgcgggggca gcatcgatga ccgcgccgcc gctgctcgac gtccgcgatc tcaccgtgga
    14941 attcgccacc cgccgcggca tcgtgagagc ggtgcagcat gtcaacatct ccatcgcaaa
    15001 aggcgagacg ctcggcgtcg tcggcgaatc cggctccggc aaatccgtca cctcctatgc
    15061 ggtgatgcgg attctcgacc gcgccggcag gatcgctgac ggctcggtgg tgttttccgg
    15121 catcgacgtc gggagcgcca gcgaagacca gatgcgcgat ttgcgcggcc gcgaaatctc
    15181 gatgattttc cagaatccgc gcgccgcgct caatccgatc cgtaaagtgg gcgaccagat
    15241 cg
//
LOCUS       DATNOI010000114        11746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_653516,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000114 DATNOI010000000
VERSION     DATNOI010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11746
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653516"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VN968_04125"
     CDS             complement(<1..537)
                     /locus_tag="VN968_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HXL28463.1"
                     /translation="MTAPAKFLFDMDFSAPDKTRERPATPSEIAQKIASAEARAYRDG
                     YDTAQREAKAESDRRTALALEEIAIGIQGIATRFSGIEIRMETEAVDVAVAVARKLCG
                     ELISGEPLVEISRLVSDCFSHLVSTPHLVVRINDSLYEAAHERIERLAKQSGFAGRLV
                     ILAEPEIANGDCRIEWADG"
     gene            complement(537..1619)
                     /gene="fliG"
                     /locus_tag="VN968_04130"
     CDS             complement(537..1619)
                     /gene="fliG"
                     /locus_tag="VN968_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HXL28464.1"
                     /translation="MAVPQTTNAHDITSVIATLAGRQKDRPKGPPLSGPKRAAILMLA
                     LGEQYGGKVWSLLDDDEVRELSMHMSTLGTVEADVVEDLLLEFVSRMSASGALMGTFD
                     GTERLLQLYLPADRVTGIMDEIRGPAGRNMWEKLSNVQEEVLANYLKNEYPQTIAVVL
                     SKLRPEHAARVLAILPEDMALDVVGRMLRMEAVQKEVIERVEQTLRTEFMSNLSQTRR
                     RDAHEVMAEIFNNFDRQTETRFITSLEEDNRESAERIKALMFTFDDLVKLDAASAQTL
                     MRHVDKDKLGIALKSANEDVRGFFLGNMSSRAGKMLLDDMAALGPVRLRDVDEAQALL
                     VNLAKDLAAKGEIMLTKNRADDELVY"
     gene            complement(1621..3228)
                     /gene="fliF"
                     /locus_tag="VN968_04135"
     CDS             complement(1621..3228)
                     /gene="fliF"
                     /locus_tag="VN968_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HXL28465.1"
                     /translation="MQGLAGFLKGLGASRLMAMIAVTAALISFFGFVIMRVTTPQMTT
                     LFTDLSTEDSSGIIKDLERQAIPFELRNDGAVIMVPKDKVTRLRMKLAEGGLPKGGGV
                     GYEIFDKSDALGTTSFVQNINHLRALEGELARTIRAIDRVQAARVHLVLPERPLFSRE
                     TPEPSASIVVRVRGALEPQQIRAIRHLVASAVNGLKPQRVSIVDEAGQLLADGATGDA
                     DNSVGDERRAGFEKRMRNQIEAIVSSVVGAGRARVQLSADFDYNKITQTSDKFDPEGR
                     VLRSSQTREESSATLETNGQVTVNNELPGNQRQDTTTPARDQSKKSEETNNYEISRTT
                     KTEVTEVGRVNRISVAVLVDGSYVKNDKGEMVYQERGKEQLDRIAALVRSAIGFDQKR
                     GDQVEVVNLKFAEAPAVVPVAEPTGLLGMLQFTKDDVMYIIELGVMMLLGLVVLFMVI
                     RPLVKRILASEVIPALAHDSLPALTDGSDAGQSLVGANATAQMIDVAQVQGQVHAQSV
                     HRVGELAERNPNETATIIRQWLTEPAT"
     gene            complement(3405..3680)
                     /locus_tag="VN968_04140"
     CDS             complement(3405..3680)
                     /locus_tag="VN968_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HXL28466.1"
                     /translation="MTEPHRPRVKYVIGPDGSPLTIADLPAPGTKRWVIRRKAEVVAA
                     VRGGLLSLEEACSRYTLTVDEFLSWQFSIDQHGLAGLRTTRIQQYRQ"
     gene            complement(3882..4583)
                     /locus_tag="VN968_04145"
     CDS             complement(3882..4583)
                     /locus_tag="VN968_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HXL28467.1"
                     /translation="MTTTAATNAASPIVSGTTPAPTSSSSGSSLNPTTNTTLAGNFQT
                     FLTLLTTQLQNQNPLDPLDTNQFTQQLVQFAGVEQQLKTNDQLTTLVSLQKTAQSTQA
                     LGFVGKNAMVDGSTTTMTNSSANWILGVKTNSTVNISIANSTGQTVFTGSYPVNAGDG
                     QAFAWDGKGNDGTQWPDGKYTLTAQAADGSGNSVAVSTEIVGVVSSVDLTQSPPLLSI
                     NGKNYTVDQIKGIAG"
     gene            complement(4594..5463)
                     /locus_tag="VN968_04150"
     CDS             complement(4594..5463)
                     /locus_tag="VN968_04150"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HXL28468.1"
                     /translation="MTVAATGAATKPATPKSALLKAPAAAKAGAAPSSDSAAAPGTAD
                     ASATAISTGTAHADVAPQPASAGKEKAENAIVDAAKTDAPAGPSSTPGAAVAAHQPSP
                     IPEAAQALTGSSDAGVQPTGTIQPQLPAAAAAAAPAGPFSVTAATNVAVPLSGLALEI
                     AASARSGKSRFEIRLDPADLGRIDVRIDVDRSGQVTSHLTVEKPETLSMLRQDAAQLQ
                     RQLDDAGFKTGNGGLQFSLRDQSSSNQNNGDETGRNAQRLVVSSEDPTPAAVVGRTYG
                     RMLGSSSGVDIKV"
     gene            5423..6205
                     /locus_tag="VN968_04155"
     CDS             5423..6205
                     /locus_tag="VN968_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28469.1"
                     /translation="MAGFVAAPVAATVTSVATVASDCVAGRFTDAAAVALAAVWTSIF
                     VPATAAAAVAGAVAAVATAAPESALTWAADAAGAPVAAVLAAMAAAAIASGAVALPDD
                     GEAAGTIVDAMATGITTATGVGVVAAGPSCCAGLAGLALASVAGLSLEDGFAVDFVSS
                     VFEVSDFALERWAPSAFALASALALASASERCLAALRSPESAPAVWSAAEVSRDRLMS
                     AVVDVSSERRCGADGSGARPLLLVSAVALLSTSAAKLSFPGG"
     gene            6652..7857
                     /gene="mnmA"
                     /locus_tag="VN968_04160"
     CDS             6652..7857
                     /gene="mnmA"
                     /locus_tag="VN968_04160"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXL28470.1"
                     /translation="MLNSLDLEGRPQDTRIVVAMSGGVDSSATAALLKSEGYDVVGIT
                     LQLYDHGAATHRKGACCAGQDIHDARNVAERIGIPHYVLDYENRFRESVIDNFADSYA
                     SGETPVPCIECNRSIKFRDLLKTARELGASALATGHYVASRRLSDGSRALTCAADADR
                     DQSYFLFATTREQLDYLRFPLGDMTKPQARELARRFGLSVADKQDSQDICFVPTGRYT
                     DIISRLRPNAVEPGDIVDLDGRVIGRHQGIVHFTVGQRRGLGIASSAPLYVVRLDAAG
                     RRVVVGPREALRMDRIVLRDVNWIGDGALDRAVGEGLEIFVRVRSTRAPQPAWLRAAD
                     NGYEVELVAGEEGVSPGQACVFYDAPSGQARVLGGGFIQSATAKTATAGVAARGSLAQ
                     PSLVEAMRG"
     gene            7876..8514
                     /locus_tag="VN968_04165"
     CDS             7876..8514
                     /locus_tag="VN968_04165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXL28471.1"
                     /translation="MASDIDRAGVAKAYERWAPVYDLVFGKVFDHGRQSTIAEADKIG
                     GRILDVGVGTGLSLSDYSPTTKLCGVDISEPMLRKAQQRVRALGLCNVETLAVMDAKS
                     LAFADSSFDAVVAQYVITAVPDPEATLDDFIRVLKPGGELILVNHIGAESGPRRIFEL
                     AFAPLARRLGWRPEFPWARLTDWAAKHGGVSLTERRPMPPMGHFSLIRYRKS"
     gene            8567..8929
                     /locus_tag="VN968_04170"
     CDS             8567..8929
                     /locus_tag="VN968_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28472.1"
                     /translation="MRMKHSLLLFGVLMAASGVAAAQAPPTPATPPAQTAPPAPARAS
                     DCAPMQSMPQGNIAPQGTTTGQSSEPLGDKLAKSDGVLCPPAGVDPQIRAPTPDTGNT
                     PVIPPPGSPGGDPNVRPK"
     gene            9051..9127
                     /locus_tag="VN968_04175"
     tRNA            9051..9127
                     /locus_tag="VN968_04175"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9085..9087,aa:Met,seq:cat)
     gene            9301..9513
                     /locus_tag="VN968_04180"
     CDS             9301..9513
                     /locus_tag="VN968_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28473.1"
                     /translation="MLGGKGGQSFDIGAVLLDRSGMQCDDIGRVGAFQLAVYIGALDF
                     KRLDPRHQGGEVDALRYRVDQALKFA"
     gene            complement(9652..10698)
                     /locus_tag="VN968_04185"
                     /pseudo
     CDS             complement(9652..10698)
                     /locus_tag="VN968_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(10837..11058)
                     /locus_tag="VN968_04190"
     CDS             complement(10837..11058)
                     /locus_tag="VN968_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28474.1"
                     /translation="MRPINSRRFDHAPELHALFFLRRVPLERWKGRWLIGITWIGDDV
                     KGLLVANSNAIRSGDRVILAMVSSDASGR"
     gene            11498..11584
                     /locus_tag="VN968_04195"
     tRNA            11498..11584
                     /locus_tag="VN968_04195"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11542..11544,aa:Met,seq:cat)
BASE COUNT         1980 a         3594 c         3780 g         2392 t
ORIGIN      
        1 gccgtcggcc cactcgatcc tgcaatcgcc gttcgcgatt tccggttctg ccaggatcac
       61 gaggcggccc gcgaaaccgc tttgtttcgc cagccgctcg attcgttcgt gtgcggcctc
      121 gtagagggaa tcgttgatcc gaaccacgag atgcggcgtt gaaaccaggt gggaaaaaca
      181 atcgctcacc agccgggaga tctcgaccag cggctcgccg gaaatcaact cgccgcacag
      241 cttgcgcgcc accgcgaccg cgacatcgac ggcttcggtc tccatccgga tttcgatgcc
      301 ggagaaccgc gtcgcgatac cttgaatgcc aattgcgatc tcctcgagcg ccagcgcagt
      361 gcggcggtcg ctttcggctt tcgcctcgcg ctgcgcggta tcgtagccgt cgcgataggc
      421 gcgggcctcg gcggatgcga tcttctgcgc gatttccgaa ggcgtggcgg gccgttcgcg
      481 cgtcttgtcc ggcgcggaga agtccatgtc gaacaggaat tttgcggggg cggtcatcag
      541 tacaccagct cgtcgtcggc gcggttcttg gtcagcatga tttcaccctt ggccgcgagg
      601 tcctttgcga gattgacgag caacgcctgc gcctcgtcga catcgcgcaa ccgtaccggt
      661 cccagtgccg ccatgtcgtc gagcagcatc ttgccggcgc gggacgacat gttgccgagg
      721 aagaagccgc ggacatcctc gttggcgctc ttaagcgcga tgccgagctt gtccttgtcg
      781 acgtggcgca tcagggtctg cgccgatgcg gcatccagct tgacgaggtc gtcgaaggtg
      841 aacatcagcg ccttgatgcg ttcggcggac tcgcggttgt cttcctccag cgacgtgatg
      901 aagcgggtct cggtctggcg gtcgaaattg ttgaagattt cggccatcac ttcgtgggcg
      961 tcgcgacggc gggtctgcga caggttcgac atgaattcgg tgcgaagcgt ctgttcgacg
     1021 cgttcgatca cttccttctg caccgcctcc attctcagca tgcggccgac cacatcgagc
     1081 gccatgtctt cgggcaggat cgcgagcacc cgggcggcgt gctccggcct cagcttcgac
     1141 agcaccacgg cgatggtctg cgggtattca ttcttgagat agttggcgag cacctcttcc
     1201 tgaacgttgg agagcttctc ccacatgttg cggcccgcag ggccgcgaat ttcgtccatg
     1261 atgccggtca cgcgatcggc gggcaggtac agctgcagca gccgctcggt gccgtcgaaa
     1321 gttcccatca acgcgccgga tgccgacatc cgcgagacga attcgagcag caggtcttcc
     1381 accacgtcgg cttcgacggt accgagcgtc gacatgtgca tcgacagttc gcgtacttcg
     1441 tcgtcgtcga gcagcgacca gactttgccg ccatattgtt cgcccagcgc cagcatcagg
     1501 atcgcggcgc gcttgggtcc gctcagagga gggcctttcg ggcggtcctt ctgacggccc
     1561 gccagcgtgg cgatgacgct ggtaatatcg tgggcgttgg tggtttgcgg tacagccatg
     1621 tcaggtcgcc ggctcggtta gccattgacg aatgatggta gcggtctcat tcgggtttcg
     1681 ctcggccagt tcgccgacgc gatggaccga ctgcgcatgg acctggccct gaacctgggc
     1741 gacatcgatc atctgcgcgg tggcattggc accgacgagg ctctggccgg cgtcgctacc
     1801 atcggtcaac gccggcaaac tgtcgtgcgc aagcgcgggg atcacttcgg aagcaaggat
     1861 gcgcttgacc agcggccgga tcaccatgaa caacaccacg aggccgagca gcatcatgac
     1921 gccgagctcg ataatgtaca tcacgtcatc cttggtgaac tgcagcatgc cgagcagtcc
     1981 ggtcggctcg gcgaccggta cgactgcggg cgcttcggcg aatttgaggt tcacgacctc
     2041 gacctgatcg ccgcgcttct gatcaaagcc gatcgccgag cgcaccagcg ccgcgatacg
     2101 gtcgagctgt tccttgccgc gttcctgata gaccatttcg cctttgtcgt tcttgacgta
     2161 gctgccgtcg accagcacgg cgaccgagat gcggttgacc ctgccgacct cggtcacctc
     2221 ggtctttgtg gttcgggaga tttcgtaatt gttggtctcc tcgctcttct tgctctggtc
     2281 gcgcgcgggc gtcgtggtgt cctggcgctg attgcccggc aattcgttat tgacggtgac
     2341 ctgaccgttg gtttcgaggg tcgcgctgga ttcctcccgg gtctggctcg aacgcagtac
     2401 ccggccttcg gggtcgaact tgtccgaggt ctgggtgatc ttgttgtagt cgaagtcggc
     2461 cgagagctgg acgcgggcgc gtcccgctcc gaccaccgaa gaaacaattg cctcgatctg
     2521 attgcgcatc cgcttctcga agccggcccg gcgctcgtcg ccgacggagt tgtcggcgtc
     2581 gccagtcgcg ccgtcggcga gcaattgccc ggcttcatcg acgatcgaca cccgttgcgg
     2641 cttcaatccg ttgacggcgg aagcgaccag atggcggatg gcacggatct gctgcggctc
     2701 gagcgcgccg cgcacgcgca ccacgatcga ggctgatggt tcgggcgtct cgcgtgaaaa
     2761 cagcggccgc tcgggtagca ccagatggac gcgggcggcc tggacgcggt cgatggcgcg
     2821 gatggtgcgg gccaattcgc cctcgagcgc gcgcaaatgg ttgatgttct ggacgaagct
     2881 ggtggtgccg agtgcgtcgg acttgtcgaa aatctcgtag ccgacgccgc cgcccttggg
     2941 caagccgccc tctgcaagct tcatgcgcag ccgggtgacc ttgtccttcg gcaccatgat
     3001 gacggcgccg tcgttgcgca gctcgaaggg tatcgcctgg cgttccaggt ccttgatgat
     3061 cccggaggaa tcttcggtgc tgaggtcggt gaagagggtg gtcatctgcg gggtggtgac
     3121 acgcatgatg acaaagccga agaagctgat cagcgcggct gtcaccgcga tcatcgccat
     3181 cagccgtgac gcgcccagac ccttcaggaa acccgcaaga ccctgcaaaa ccaacccgcc
     3241 cctgagattc ggccgcaagc gccgactggg caattattgc ctatgggatg gtttcgatat
     3301 ggttaacgca ggttaagagc cgcggcgaaa gtttcaagat ggaaaagatc ggcttcgcga
     3361 aacgaagccg attctcatcc aaagccgtcg gctctggaaa gcgtttattg acgatattgc
     3421 tggatgcggg tggtgcgcag tccggccagg ccgtgctggt cgatggaaaa ctgccaggac
     3481 aggaattcat cgacggtcag cgtgtagcgg ctgcaagcct cctcgaggga gagcaggccg
     3541 ccgcgcactg cggcaacgac ttcggcctta cggcgaataa cccaccgttt ggtcccgggc
     3601 gcgggtagat cggcaattgt tagcggactg ccgtcaggcc cgatgacgta ttttaccctc
     3661 gggcgatggg gttctgtcat ggcgtactca caaactctca accactgaac tcacaggtaa
     3721 caacctacgc gccgcgactt aaaatttacc taagcccaag actgcaatca gagcctcctt
     3781 gaaattggct gcaaaacctt tggcccggcg tctggaggac gccgggcgtg aggcagatcg
     3841 tgaatcaatc gttaattttt ccgcggctcg atcgcctggg attagcccgc gatgcccttg
     3901 atctggtcga cggtgtagtt tttaccgttg atcgacagta atgggggcga ctgggtgaga
     3961 tccaccgaac tgaccacgcc cactatctcg gtggagacag ccacggaatt gcccgagccg
     4021 tccgctgcct gagcggtcag cgtgtacttg ccgtccggcc attgcgtgcc gtcgttgccc
     4081 ttgccgtccc aggcgaaggc ttggccatcg ccggcgttga cggggtagct gccggtgaag
     4141 acggtttgtc cggtgctgtt ggcgatggag atgttcacgg tggaatttgt cttgactccc
     4201 agaatccagt tcgctgacga attggtcatg gtcgtggtgc tgccgtcgac catcgcgttc
     4261 ttgccgacga aaccgagcgc ctgcgtcgat tgggcggttt tctgcaacga gaccagcgtg
     4321 gtcagttgat cgttggtctt gagttgctgt tccacccctg cgaactgcac cagctgctgg
     4381 gtgaactggt tggtgtccag cggatcgagc gggttctggt tctgcaattg cgtcgtcaag
     4441 agcgtcagaa acgtttgaaa gtttccggcg agtgtcgtgt tggtggtcgg attcagagag
     4501 gagcccgacg aggatgatgt cggagcgggt gttgtccccg aaacgatcgg gctggccgcg
     4561 tttgttgctg ctgttgttgt catgtccgtc tcctcagacc ttgatgtcga cgccgctgct
     4621 cgatccgagc atgcggccat aggttcgtcc cacgaccgcc gcaggggtgg ggtcttcgct
     4681 gctgacgacc agccgctgcg cgttgcggcc ggtttcgtcg ccgttgttct ggttggacga
     4741 ggactggtcg cggaggctga attgcaggcc gccgtttccg gtcttgaagc cggcgtcatc
     4801 gagctggcgc tgcaactggg ctgcgtcctg gcgcagcatt gacagcgttt ccggtttttc
     4861 gaccgtcaaa tgcgaggtca cctggccgct gcgatcgaca tcgatgcgaa catcgatgcg
     4921 gccgagatcg gcgggatcga gcctgatctc gaaacggctc ttgccgcttc gtgccgaggc
     4981 ggcgatctcg agtgcgagcc cgctcaatgg cacagcgaca ttcgttgccg ccgtgacgct
     5041 gaaggggccg gccggagcgg ccgcggccgc ggcggcaggg agctgcggct ggatcgtgcc
     5101 tgtcggctgc acccctgcat cggatgaacc ggttagcgcc tgtgccgcct cggggatcgg
     5161 cgaaggttga tgcgccgcca cggcggctcc cggcgtcgat gagggaccgg cgggggcgtc
     5221 ggttttcgcc gcatcaacaa tggcgttttc agccttctcc ttgccggcgc tggccggttg
     5281 cggagcgacg tcggcgtggg cggtcccggt cgatatggcc gtcgcggacg catcggctgt
     5341 acccggcgcg gcggcggaat cgcttgatgg cgcggcaccc gcttttgcgg cggctggcgc
     5401 cttgaggagc gcggatttcg gcgtggcagg ttttgtcgca gcaccggtgg cggccactgt
     5461 cacgtcggtc gccacggttg catcggactg cgtagccggc cgattcactg atgcggcggc
     5521 cgtcgcgcta gccgcggtct ggacctcgat ctttgtgccg gcgaccgcag ctgcggcagt
     5581 tgccggcgcg gttgctgccg tcgctaccgc cgcgccggaa tcggccttga cctgggctgc
     5641 ggatgcagcc ggtgcgccgg tggccgccgt gctggcagcg atggccgctg cggcgatcgc
     5701 cagcggtgcg gtagccttgc cggacgatgg cgaagctgcg ggaacgatcg tcgatgcgat
     5761 ggcgaccgga atcacaaccg cgaccggggt tggcgtcgtc gctgccgggc cgtcctgctg
     5821 cgccggtttg gccgggttgg ctttggcatc ggttgccgga ctatctttgg aagacggctt
     5881 tgcggttgat ttcgtgtcgt ctgttttcga agtatcggat ttcgccctgg agcgctgggc
     5941 cccatctgcg ttcgcgttag cgtcggcgtt ggcattggca tcggcgtcgg aacgttgttt
     6001 ggcggcgctg cgatcgcccg aatcggctcc tgctgtctgg tcggcggccg aggtgtcacg
     6061 cgatcgtcta atgtccgcag tggtcgatgt gtcgtcggag cggcgttgcg gcgcggatgg
     6121 ctccggcgcg cgaccgttgt tactggtgtc ggcggtcgcg ttgctgtcca ccagtgccgc
     6181 gaaactatcg tttccgggcg gctgatcgga ccgcgcaggc ctcggtagcg gcaagccctg
     6241 aaaggacggg ctggctgctg cttctgacgt tgaactgtac acggggcggc ctcgcgcaat
     6301 gaaactggaa ctaagatcag caaggaccgg gccatcgcgt ttctcaaaaa atattcatat
     6361 atttcagtat gttacaacca tgtccggcta actacggaac tgccatgggg tccgcttttt
     6421 cctgcccagc ggcaagattt gccggttgct gtgcccaaaa cggggtcgcc tgattgcctc
     6481 gccgggatac ggcctatatt aaagaacagc tttgcagtca ttccgatccg gcgaacgcgc
     6541 tcccgggacc gctgcaaagc cttctaccga caaggttttt tcactattcg caaaaatggc
     6601 ggtctaatcg cgaagggctc gatgccgccg ccatcgcaac cggccggagc catgctcaac
     6661 agtctggatc tcgaagggcg tccacaggat accaggatcg tggtcgccat gtccggcggc
     6721 gtcgattcgt cggcgacggc tgccttgttg aaatccgagg gctatgacgt cgtcggcatc
     6781 acgctgcagc tttacgatca cggcgccgcc acccatcgta agggcgcctg ctgcgccggg
     6841 caggacatcc atgacgcgcg caacgtcgcc gagcggatcg gcattccgca ttacgtgctg
     6901 gactacgaaa accgctttcg tgaatcggtg atcgacaatt tcgccgacag ctatgcttcc
     6961 ggcgagacgc cggtgccctg catcgagtgc aaccgctcga tcaagttccg cgatctgctc
     7021 aagaccgcgc gcgaactcgg cgcttcagcg cttgcgaccg ggcactatgt ggcgtcgcgc
     7081 cgcctgagcg atggatcgcg cgcgcttact tgcgccgccg atgccgatcg cgaccagagc
     7141 tatttcctgt tcgcgaccac gcgcgagcaa ctcgactatc tgcgctttcc gctgggcgac
     7201 atgaccaagc cgcaagcgcg cgaattggcg cggcgcttcg gcctgagcgt cgcggacaag
     7261 caggacagcc aggatatctg cttcgtgccg accgggcgtt ataccgacat catcagccgg
     7321 ttgaggccga atgccgtcga gcccggcgac atcgtcgacc tcgacggccg cgttatcggc
     7381 aggcatcagg gcatcgttca tttcaccgtc ggccagcggc gcggattggg catcgcctcc
     7441 agcgcgccgc tgtatgtggt gcggctcgac gccgccggcc gccgcgtcgt ggtgggcccg
     7501 cgcgaggcgt tgcggatgga ccgcatcgtg ctgcgcgatg tcaactggat cggcgacggc
     7561 gcgctcgatc gcgccgtcgg cgagggtctg gagattttcg tgcgcgtccg atcgacccgg
     7621 gcgccgcagc cggcctggct gcgcgcggcc gacaacggtt atgaagtcga actcgtcgcc
     7681 ggcgaagagg gcgtttcccc cggccaggcc tgcgtgttct acgatgcgcc ctcgggccag
     7741 gcgcgtgtgc tcggtggcgg attcatccag agtgcgaccg cgaagaccgc gacggccggg
     7801 gtcgcggcgc gcggctccct cgcgcagcct tcgctggtcg aggccatgcg cggataaaat
     7861 tttcagggcg ggggcatggc gtcagatatc gaccgcgcgg gggtcgcaaa ggcttatgag
     7921 cgctgggcac cggtctacga tctggtattc ggcaaggttt tcgatcacgg ccgccaatcg
     7981 accattgcgg aagccgacaa gatcggtgga cggattctcg atgtcggcgt cggcaccgga
     8041 ttgtcgctgt cggattattc gccgaccacg aaattgtgcg gcgtcgatat ttccgagccg
     8101 atgctgcgca aggcgcaaca gcgcgtgcgc gcgctcggcc tctgcaatgt cgagacgctt
     8161 gcggtcatgg acgccaagag tttggccttc gcggattcgt ctttcgacgc ggtggtggcg
     8221 caatatgtca tcaccgcggt gcccgatccc gaagccacgc tggatgattt catccgcgtg
     8281 ctgaaacccg gtggcgagct cattctggtc aatcacatcg gcgccgagag cggtccgcgc
     8341 cgcatcttcg aactggcgtt cgcgccgctg gcgcggcggc tcggctggcg gccggaattt
     8401 ccatgggcgc ggctgacgga ctgggccgcc aaacacggcg gcgtcagcct gaccgagcgc
     8461 cgcccgatgc cgccaatggg ccatttttca cttattcgct accgcaaatc ttgagattac
     8521 cgctcgctta ggcggaacat ccggccgtgc agaccgtttt agccgcatgc ggatgaaaca
     8581 ctcactcttg cttttcggcg ttctgatggc ggcgtccggc gtggcggctg cgcaagcccc
     8641 gcccacgccg gcgacgccgc cggcgcaaac cgcaccacct gcgcccgccc gcgccagcga
     8701 ttgcgcgccg atgcaatcga tgccacaggg aaacatcgcc ccgcagggca cgacgaccgg
     8761 tcaaagcagt gaaccactgg gcgataaact cgccaaatcc gacggcgtgc tgtgcccgcc
     8821 cgcgggcgtc gacccgcaga tccgggcacc gacgccggat accggcaata cgccggtgat
     8881 accgcccccc ggcagccccg gcggcgatcc gaacgtccgt ccgaaatagc accctttaca
     8941 atcacttggc ggaaagggcg ggtttgcttg acatgcccaa gacctgcttc ttatatgccg
     9001 cgcgttcgcg aactgcggcg gtttataagc cctgggacga tgaacgccgt ggcggggtag
     9061 ctcagctggt tagagcacgg gaatcataat cctggggtcg ggggttcgag tccctctccc
     9121 gctaccactg atcccccctc cctcagaacg gctcgggaac aaatccgaat tcgtcaattg
     9181 tgctagtcgt ccctgcactt cgacgttgaa gcctcggccg tacggttcgg cgtgtatgat
     9241 gactccggcc accagccgcc gcaccgcttc gatcagttcc gccgattcca caaagtcgct
     9301 gtgctcggcg gcaagggcgg ccagtctttc gacatcggcg cggtattgct cgatcgcagc
     9361 gggatgcagt gtgacgatat cggccgcgtt ggcgcatttc agcttgccgt ctatatcggc
     9421 gcgctcgatt tcaagcgcct tgatcctcgg caccaaggtg gcgaggtcga cgccttgcgt
     9481 taccgcgtcg atcaagcgct caagttcgcg tgagacctcg gcattgcggc gtgtcagctt
     9541 gctcatctgg tcgccagctt cgcgtgcaag gcgtcgccgc tcgtcgttat aggccttaat
     9601 gtattctgta atcgcaatcg gattggacag ctcgtctttc agtcccgcaa acacggccgc
     9661 ttcaatggcg tcgcggttga ccttccggct gttggtgcag gacccactct ccttgtaggt
     9721 gccacattgc agtcgcgcgg accgcttgcg attgccgatc gacaccattc cgccgccgca
     9781 ggatccacac cgcagcaagc ccgaaagcgg acgccgtggc gcgcgggatt gagtcgcgcc
     9841 gaggtgcgag cgcttcagct taatggcttg cgccgcgttc caggtcacat catcgatgat
     9901 gcgcagttgc ggcgcctcgg taattcgatg ctgctccttc gcattgattc gcgagatccg
     9961 tttgcccgtg gtcggatcct tgaccatccg caccttgttc cagacgatac ggccggcata
    10021 aagctcattg agcagcatgc cgtggccgcg cgcgttgttg ccattgatgg tagatgcgtt
    10081 ccacctagtg ccgcgcggcg ccgcaatgcg atcggcattg agttcggcag caatggcgcg
    10141 aggcgtgacg ccggcaacaa agcgcgcgaa gattcgctgc acgatcccgg cctgcgcgtc
    10201 gtcgatgacg agctcgcccg ccgaacccgg gacgatggaa tagccatacg cgcgtccgcc
    10261 ggcgtggcga ccgtcgcgga tcacgccctc gagtccgcgc cgtacatgca gcgccaagtt
    10321 ttcgagatac atttcgccca tcaatgcccg cagcgcgcca tcgatcttgg tcactttccc
    10381 ggtcgcggta tggatggtga ttccgagaaa atcgagttcc ttgcgagcag cgtgataatc
    10441 ggcctggtcg cgaaaaatgc gatccatgtc ttcggcaacg acgacgtcga acagccttgc
    10501 cgccgcagcg cgcatcagcg cgatgaagcc gggacgattc tttgccgacg atgcggacac
    10561 agcgcggtcc tcgaaggtca acaccaccgt catgccattg cgagcgcaga cctcgcggca
    10621 gaacgcgatc tggtcgtcga ccgatttatc gttttgcagg tcgctcgaaa aacgagcgta
    10681 gatggcgacg cggatcatgc actccgctcg gtcgcgcggg cttgaagtga tcggcgcgcg
    10741 atttctgctt catgatctat ccgggctcgc agtttggcaa gcgccctgac cacggcaaga
    10801 acgtcctccc cgggcggaat ttgcggtttt tgtgggttaa cggccggagg catcgctcga
    10861 aaccatggcg agaatgaccc gatcgccgct ccggatggca ttgctgttgg ccaccaggag
    10921 gcccttcacg tcgtcgccga tccaagtgat gccgatcagc cagcggcctt tccagcgctc
    10981 caacggcact cgccggagga agaaaagcgc gtggagttcg ggtgcgtgat cgaagcggcg
    11041 cgaattgatc ggcctcatgc tgccccgtcg gttgacgaaa aagcggcgcc ccgcttggcg
    11101 cagcagccgc cgcagtgacc ggcgcgggag tgatcgccgg attggagccg acacgtcggg
    11161 cctgcgtcag cgcggcgtcg atcagtgcga cgatatcgcc gacgcgccgg ttcttgatcc
    11221 acggtcaatg aagtggtgaa gctaccaacc atgcgtgcaa catgccgccg ccagcatatc
    11281 cacgaaaggg cggcttacct tgcgtcgcaa agacggaaag cttagcgcgt caatcggtgg
    11341 tttcgtccga tggtgcgacc tttcgctaat gcgcccgata gctcaaaaca aatgcgtttc
    11401 agggtatcgt cggaattggc ctagacttaa aatcatttca gtctgggtcc tgatctgggg
    11461 aagttttcta gcggttggac cggcggccag aataggtcgc tcgtgactag cggttcgatg
    11521 ccggcagtcg gtcgtcaggc tcataacctg aaggtcatag gttcaaatcc tatccccgca
    11581 accaaataag atgctgattt ttatgcataa atgcgaaaag ccgctcaatt gagcggcttt
    11641 ttgttatcga gaatcgccac cgagtcgcca cgcgtgctgt aaatgctgtg ttggcttcga
    11701 gcaggtcggt cctgtatcgt gggttcgaat ccctccctct ccgcca
//
LOCUS       DATNOI010000115         2464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2257149,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000115 DATNOI010000000
VERSION     DATNOI010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2257149"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VN968_04200"
     CDS             complement(<1..248)
                     /locus_tag="VN968_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HXL28475.1"
                     /translation="MSKPPPEEDPHNRIVAVTLDEESIGRSGPDIEHERAIAIYDLIE
                     KNLFAPEGADAGPFALHLGITGNRLMFDIRREDGTPVVA"
     gene            complement(245..1540)
                     /gene="hisD"
                     /locus_tag="VN968_04205"
     CDS             complement(245..1540)
                     /gene="hisD"
                     /locus_tag="VN968_04205"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXL28476.1"
                     /translation="MPVRLDRNSADFVQRFADFLAMKREVSADIESATRAIVDDVAAR
                     GDLALIEATRKFDRLDLKPGGLRITPAEIDAAVKACDAGTLDALKFARDRIEVFHRRQ
                     LPKDERFTDALGVELGWRWSAIDAVGLYVPGGTAAYPSSVLMNAVPARVAGVARVVMV
                     VPSPDGKLNPLVLAAADLGGVSEIYRVGGAQAVAALAYGTDTIAPVAKIVGPGNAYVA
                     AAKRLVFGKVGIDMIAGPSEVLVIADATGNAGWIAADLLAQAEHDVNAQSILITDSAA
                     LADEVERAVESQLATLPRADIARASWNEFGAIILVKQLDAAIELANAIAAEHLEILTS
                     DPESVAAQIRNAGAIFLGPHTPEAIGDYVGGSNHVLPTARSARFSSGLGVLDFMKRTS
                     ILKCGPDQLRALGPAAMTLGKAEGLDAHARSVGLRLNLT"
     gene            complement(1783..2238)
                     /locus_tag="VN968_04210"
     CDS             complement(1783..2238)
                     /locus_tag="VN968_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HXL28477.1"
                     /translation="MTAQLKLIALDADDLAVISTHVQDARVQTSDIIWRQGEKRLLVG
                     MNRLDWEQTLFGETAPRRLVAVLRFDRVLSCKSRNIDLGAPETVLELVGIEFHPGEAP
                     GGSAVLMFSQGGALRLGVECLECELTDLGADDLGTSDLGEGAEPSGWDA"
BASE COUNT          427 a          825 c          776 g          436 t
ORIGIN      
        1 gccaccacgg gcgtgccgtc ctcgcggcgg atatcgaaca tcagccggtt gccggtgatg
       61 ccgaggtgca aggcaaacgg ccccgcatcg gcgccctccg gcgcgaacag atttttctcg
      121 atcaggtcgt agatcgcaat cgcgcgctcg tgctcgatgt cgggaccgga acgcccgatc
      181 gattcctcat ccagcgtcac cgcgacgatg cgattgtgcg gatcctcctc tggcggcggc
      241 ttgctcatgt gaggttaaga cgcagtccga ctgaacgcgc gtgggcatcg aggccttcgg
      301 ccttgcccag cgtcatcgcc gccggtccca atgcccgcaa ctggtccggc ccgcatttga
      361 ggatcgacgt tcgcttcatg aagtcaagca ccccgagccc agaggagaac cgggcggacc
      421 gcgccgtcgg cagcacgtgg ttggagccgc cgacataatc gccgatcgcc tcgggggtgt
      481 ggggccccag gaagatcgcg ccggcattgc ggatttgcgc ggcgacgctt tccggatcgg
      541 aggtcaagat ctccagatgc tcggcggcga tggcgttggc aagctcgatc gccgcgtcga
      601 gctgcttcac caggatgatg gcgccgaact cgttccacga agcccgcgcg atatcggcgc
      661 gtggcagggt tgcgagttgc gattccaccg cgcgttcgac ctcgtccgca agcgccgcac
      721 tgtcggtgat caggatcgac tgcgcgttga cgtcgtgctc ggcctgcgcc agcagatcgg
      781 ccgcgatcca gccggcattg ccagtggcat cggcgatcac aagcacttcg gagggaccgg
      841 cgatcatgtc gatgccgacc ttgccgaaca ccaatcgctt ggcggcggcg acataggcgt
      901 tgccggggcc gacgattttt gcaactggcg cgatggtgtc cgtaccgtaa gcaagggcag
      961 ccaccgcctg cgcgccgccg acgcgataga tctcggacac gccgccgaga tcggccgccg
     1021 ccagcaccaa gggattgagc ttgccgtcgg gcgacggcac caccatcacc acgcgcgcga
     1081 cgccggcgac gcgtgccggc accgcgttca tcagcaccga ggacggatag gcggcggtgc
     1141 ctcccggcac gtacagaccc acggcgtcga tcgcgctcca gcgccagccg agctcgacgc
     1201 cgagcgcatc ggtgaagcgc tcgtccttcg gcaattgccg ccgatggaaa acttcgatcc
     1261 ggtcccgcgc aaatttgagc gcgtcgagcg tcccggcgtc gcaggccttt accgccgcgt
     1321 cgatttcggc cggcgtgatg cgcaagcccc caggcttgag gtcaaggcgg tcgaatttgc
     1381 gcgtcgcctc gatcagcgcc aggtcgcccc gcgccgccac atcgtcgaca atggcgcggg
     1441 tggcgctctc gatatcggcc gatacctcac gcttcatcgc gaggaaatca gcgaatcgct
     1501 ggacgaaatc ggcgctgttt ctgtctagtc gaacaggcat gttggctttc cggcggtggg
     1561 cctcattcga aggcctactg ctcaatggcg cggcgacaaa tcgccgtcaa cccttccgct
     1621 tgaggattaa gttaatgatg gcagagcgcg tcttccgtca ttccgggatg gtccgcagga
     1681 ccagacctca gatgtgcgct gcacatcggg gaatcttgag attccgggtt catcgctgcg
     1741 cgatgccccg gaatgacgct attatatcaa tgcgcgtggg tctcaggcgt cccatccgga
     1801 gggttcggcg ccttcgccga gatcgctggt cccgagatcg tcggcgccga ggtcggtcag
     1861 ctcgcattcc aggcactcga cgcccagccg cagcgccccg ccctggctga acatcagcac
     1921 cgcgctgccg cccggcgcct cgccgggatg aaactcgatc ccgaccagtt ccagcacggt
     1981 ttccggcgcc cccagatcga tgttgcgcga cttgcacgac aacacccggt caaagcgcag
     2041 caccgcgacc aggcggcgcg gggcggtttc cccgaaaagc gtctgctccc agtccagccg
     2101 gttcatgccg accagcagcc gcttttcgcc ttgccgccag atgatgtcgg aggtctgcac
     2161 gcgggcgtcc tgcacatgcg tggaaatcac ggcaaggtcg tcggcatcga gcgcgatcaa
     2221 tttgagctgg gccgtcaccg cgtgcaccta tcatttgcgc gtactctacc gcagatcagt
     2281 ccacgcaatc tgcgcaaact tgatgcaaag caaaaccgga tcccttttgc ggaatccgcg
     2341 ctcccgttca atcgctgatg cgctcgatgg aagcgccgca cgccgacagt ttttcctcga
     2401 gccgttcaaa cccgcggtcg agatggtaga cgcggttgac catggtctcg ccttcggcgg
     2461 cgag
//
LOCUS       DATNOI010000116         9380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1884089,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000116 DATNOI010000000
VERSION     DATNOI010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9380
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884089"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VN968_04215"
     CDS             complement(<1..206)
                     /locus_tag="VN968_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine nucleosidase"
                     /protein_id="HXL28478.1"
                     /translation="MTGKILVLTAIDDELDKARAPEGIEVIYSGVGKVNAASAATLAL
                     LVLRPTLVINYGTAGKISEKIHGLV"
     gene            complement(203..1369)
                     /gene="dapE"
                     /locus_tag="VN968_04220"
     CDS             complement(203..1369)
                     /gene="dapE"
                     /locus_tag="VN968_04220"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HXL28479.1"
                     /translation="MNDALSIARDLVRCPSVTPADAGALGVLEDVLKSAGFEVHRVTF
                     SEPGAADVDNLYARIGASAPHITFAGHTDVVPPGDETAWTHGAFSGDVKDGFLYGRGA
                     VDMKGGIACGVAAVLGYLAAHGGRPGKNGKGSISFLITGDEEDISVNGTIKLLRWVAA
                     RGEKFDHCVLGEPSNIEALGDCIKVGRRGSLSGTLYVDGVQGHVAYPQRASNPVPDIS
                     ALVLALSNEPLDHGSAQFQPSNLEFTSVDVGNTASNVIPGQARAKFNIRFNDHHTQDS
                     LRALVEQRLTKAAGNRIHARIVWEPSNSNVFVTKPGAFTDLAVAAIEEVTGRRPELST
                     SGGTSDARFIAGYCPVIEFGLVGQTMHQVDERTPVSDLEKLTMIYRGVLDRYFA"
     gene            complement(1510..1953)
                     /locus_tag="VN968_04225"
     CDS             complement(1510..1953)
                     /locus_tag="VN968_04225"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HXL28480.1"
                     /translation="MDWTWFLFSFEGRIDRAKFWLAAFIRFATVFSFMTIFLFVVAGI
                     LRASGNDFHSVSTRTMHPAFYLPGFPLFVIGVWLFAATSIKRLHDRGKTGWWLAPFFH
                     CSGPARQALGLDRQPTPGTSCQRAQSRRERLVLCRTVLPGRKERA"
     gene            complement(2022..2696)
                     /locus_tag="VN968_04230"
     CDS             complement(2022..2696)
                     /locus_tag="VN968_04230"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HXL28481.1"
                     /translation="MGNSYTRDEFLFSFEGRINRAKYWFALYAGMVTCLVRLAFLGFL
                     VFALDAIFGTSVKSVHLNIYDIFNDPPLFPLRVSFGDSGPAWLVSLLFYAGVTPIIVA
                     AIRLLAATAVRRLHDRNKSGWWTVPFFIAPVLLGKVGDWLGDSYPADFLRLVLIALSL
                     WGFVEILCLRGTRGPNRFGPDPHPQAPVNRSPRAAPNWEQLRELESVRRGAGPSPGEH
                     VKRGYD"
     gene            complement(2736..3323)
                     /locus_tag="VN968_04235"
     CDS             complement(2736..3323)
                     /locus_tag="VN968_04235"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HXL28482.1"
                     /translation="MDYKWFLFSFEGRINRAKYWLAALIILCGMIFVLMLLAALATIF
                     GIGGPLAINVIGISASIQLGDDDPASKASLFPQIVIIPMTLVFGWSYAAASIKRLHDR
                     NKSGWWIVPFIAATGVYGQFGDRLGGSYPAVFIGLAVFIAFIWGFVEMCCLKGTRGPN
                     RFGSDPLAPRDTRPRWDQQRELEFVPHRAGPSAGA"
     gene            complement(3495..4340)
                     /gene="dapD"
                     /locus_tag="VN968_04240"
     CDS             complement(3495..4340)
                     /gene="dapD"
                     /locus_tag="VN968_04240"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HXL28483.1"
                     /translation="MSLASLESTVNTAFDARDGISTATKGEVRDAVDHALELLDKGEA
                     RVAEREAGGKWKVNQWLKKAVLLSFRLNDMSAIPGGPGNASSWDKVPLKFEGWGENRF
                     RDAGFRAVPGAIVRRSAFIAKNVVLMPSFVNLGAYVDESTMIDTWSTVGSCAQIGKRV
                     HISGGVGIGGVLEPLQAEPVIVEDDCFIGARSEVAEGVIVRKGAVLAMGVFLGASTKI
                     IDRDSGEIFIGEVPEYAVVVPGNLPARPLKNGQPGPSTACAVIVKRVDERTRAKTSIN
                     ELLRD"
     gene            complement(4576..5271)
                     /locus_tag="VN968_04245"
     CDS             complement(4576..5271)
                     /locus_tag="VN968_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HXL28484.1"
                     /translation="MKNTSRAFSHIDTWVFDLDNTLYPHHVNLWQQVDVRIGEFVSAW
                     LKISAAEARRVQKDYYRRYGTTMRGMMTEHGVHADDYLAYVHQIDHSPLQPNPEMGLA
                     IAKLPGRKLILTNGSTDHADKVLARLGIGAHFEAVFDIIAAELEPKPAPQTYQKFLRL
                     HGVNARTSAMFEDLARNLVVPHQLGMTTVLVVPDGTREVVREDWELEGRDAAYVDHVT
                     DDLTGFLEGVARD"
     gene            complement(5332..6318)
                     /locus_tag="VN968_04250"
     CDS             complement(5332..6318)
                     /locus_tag="VN968_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HXL28485.1"
                     /translation="MSLPAAVISFLFSAVPAYADLKLCNRMSYVVEAAIGIDDKSATA
                     TRGWFRIDPAACRVVAQGTLTADRILLTARALGVYGSSPIPQSGGDTLCIAPDYFVIA
                     AARQCRSGQSPAPFTQISPTRADDGNQVAYLAEDSEYDDEQARLAGIQRLLVIAGYDA
                     APIDGVDGPKTQAALAAFLKSRGLSADIVQAPNFFTMMIDAVQSPSSTGLTWCNDTPH
                     KIMAAVATDDGKTVISRGWYGIEPGKCLHPDVTGAPKQIFSFAEAVDAANRALKVKDT
                     PLNWGGTTQLCTRESKFEINEQGDCGTRGLGVTGFAAVDMSSGGKTLRFALP"
     gene            complement(6516..7412)
                     /gene="argB"
                     /locus_tag="VN968_04255"
     CDS             complement(6516..7412)
                     /gene="argB"
                     /locus_tag="VN968_04255"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HXL28486.1"
                     /translation="MTDAPHISPLDQARILSEALPHMQQYDEETIVIKYGGHAMGAED
                     LAKAFARDIVLLEQTAINPVVVHGGGPQIAQMLQRLGIKSEFAAGLRITDAATIEIVE
                     MVLAGSINKALVGYINEAGGKAVGLCGKDGNMVTASKVTRTMVDPDSNIEKVVDLGFV
                     GEPEKVDLTLLNQLIGHDLIPVLAPLATAKSGQTFNVNADTFAGAVAGALKAKRLLLL
                     TDVPGVLDKSKKLIPELSVKDARKLIADGTISGGMIPKVETCIYALEQGVEGVVIIDG
                     KTQHAVLLELFTNQGTGTLIHK"
     gene            complement(7648..8025)
                     /locus_tag="VN968_04260"
     CDS             complement(7648..8025)
                     /locus_tag="VN968_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HXL28487.1"
                     /translation="MMPSRLFRILIVLAGIMGADGVVLAAASAHQADASRLASASSML
                     LFHATAVLAAVALADRGVIHVRIGIAAAFGFVIAAALFAGDLTLRQYAGQSLFSMAAP
                     TGGTLLIVSWLVLAVAAAWPRRG"
     gene            complement(8022..8675)
                     /gene="yihA"
                     /locus_tag="VN968_04265"
     CDS             complement(8022..8675)
                     /gene="yihA"
                     /locus_tag="VN968_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HXL28488.1"
                     /translation="MPPDIDAKLIEQGRKLFAGDWQFFWASPSIETLPPMAGMEVAFA
                     GRSNVGKSSLINALTGRNALARTSHTPGRTQELIFFEGPDNAGFRLVDMPGYGYASAP
                     KAKVASWTALIHTFLQGRSSLARVYVLIDARHGFKEVDLDVLKTLDKSAVSYQVVLTK
                     ADQVKPAELEQRAAEVQTALAKHPAAFPELLVTSSRTGAGMPELRAAMVRLLGERSR"
     gene            complement(8831..>9380)
                     /gene="yidC"
                     /locus_tag="VN968_04270"
     CDS             complement(8831..>9380)
                     /gene="yidC"
                     /locus_tag="VN968_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HXL28489.1"
                     /translation="KVKQQQEMMEIYKKEKINPIAGCLPVALQIPVFFSLYKVLFVTI
                     EMRHAPFFGWVKDLSAPDPTNLFNLFGLLHFDPTTLPLFGHYLALGVWPIIMGITMWF
                     QMKLNPTPPDPTQKMIFDWMPLIFTFMLAGFPAGLVIYWAWNNTLSVLQQSFIMRRNG
                     VKVELFDNLRATFASKKVESKT"
BASE COUNT         1832 a         2866 c         2983 g         1699 t
ORIGIN      
        1 acgaggccat gtatcttctc gctgattttc ccggccgtgc cgtagttgat caccagcgtc
       61 ggccgcagca ccagtagcgc cagcgtcgcc gcactcgccg cgttgacctt gccgaccccg
      121 ctgtagatca cctcgatgcc ctcgggcgcg cgcgccttgt cgagttcgtc gtcgatcgcg
      181 gtaagaacga ggatttttcc ggtcatgcga aatatcgatc cagtacgccg cgatagatca
      241 tggtcagctt ttccaggtcg gaaaccggcg tgcgctcgtc gacctggtgc atggtctggc
      301 cgaccagacc gaattcgatc accggacaat agccggcgat aaagcgcgcg tccgaggtgc
      361 cgccggaggt gctcaattcc ggccgccggc cggtgacctc ctcgatcgcc gcgaccgcga
      421 gatcggtgaa ggcgccgggt tttgtcacga acacgttcga gttcgacggc tcccagacga
      481 tacgcgcatg gatccggttg ccggccgcct tcgtcaggcg ctgctcgacc agcgcccgca
      541 gcgaatcctg cgtgtggtga tcgttgaagc ggatgttgaa tttggcgcgg gcctgccccg
      601 ggatcacgtt gctggcggtg ttgccgacat cgaccgaggt gaattcgagg ttcgacggct
      661 ggaattgcgc gctgccgtga tcgagcggct cattgctgag cgccaagacc aacgccgata
      721 tatccggcac cgggttcgac gcgcgctgcg gataggcgac atggccctgg acgccatcga
      781 cgtaaagcgt gcccgacaag gagccgcggc ggccgacctt gatgcagtcg ccgagcgcct
      841 cgatattgct gggctcgccc agtacgcaat ggtcgaattt ctcgccgcgc gccgcgaccc
      901 accgcagcag tttgatggtg ccgttgacgg agatgtcctc ctcgtcgccg gtgatcagaa
      961 acgagatcga acccttgcca ttttttccgg gccggccgcc atgggcggcg agatagccga
     1021 gcaccgcggc gacaccgcag gcaatgccgc ccttcatgtc gacggcgccg cgcccgtaga
     1081 gaaaaccgtc cttgacgtcg ccggagaatg cgccatgggt ccacgcggtc tcatcgcccg
     1141 gcggcaccac gtcggtatgt ccggcaaagg tgatatgcgg agctgaggcg ccaattcggg
     1201 catagagatt gtcgacatcg gcggcgccgg gttcgctgaa ggtgacgcga tgcacctcga
     1261 atccggcact tttcagcacg tcttccagca caccgagcgc accggcatcc gccggggtca
     1321 ccgaggggca gcgcacgagg tcgcgggcaa tggaaagggc atcattcatg ccctcgctta
     1381 ccatgaggcc ccggcgatgg gccagcactg tgcggaacaa attccagtgc gctatgctgg
     1441 tcccagcgcg gccgcgtatc taccggcgcc accggtgcta ccggcgccag cgggtcggag
     1501 ccgaaccggt taggcccttt ccttcctccc aggcagaaca gttcgacaaa gcaccagacg
     1561 ctcacgccga gactgagcgc gctgacaaga agtgccaggg gtgggttgtc tatccagacc
     1621 gagagcttgt cgagcaggcc cggagcaatg aaaaaacggc gcaagccacc aacctgtctt
     1681 gccgcgatcg tgaagcctct tgatcgaggt cgcggcaaac agccaaacac cgatgacgaa
     1741 caggggaaat ccaggcagat agaacgccgg gtgcatcgtc ctggtcgaga cactatggaa
     1801 atcattgcct gaagcacgca ggatgccggc aacgacgaac aggaagatcg tcatgaacga
     1861 aaagacggtg gcaaacctga tgaacgcggc aagccagaat ttggcgcggt cgatgcggcc
     1921 ttcaaagctg aacagaaacc aggtccagtc catgtttaaa gctccggagg tcgggaggcg
     1981 ccctcgtttt cggttatggg tcggcggcaa tggagagggc gtcagtcata acctcgcttg
     2041 acatgctcgc ccggcgatgg gcccgcaccg cgccgaacag attccagctc tctcaactgc
     2101 tcccaattcg gggctgcacg cgggctgcga ttgaccggcg cctgcgggtg cgggtctggg
     2161 ccaaatcggt tgggccctct tgtccctctc agacacagta tttcgacaaa accccagagg
     2221 ctgagagcga tcaggacgag cctgaggaag tccgccgggt aggaatcgcc aagccagtcc
     2281 cccaccttgc cgagcagaac aggagcaatg aagaacggga cggtccacca gccgcttttg
     2341 ttgcgatcgt gaagccgcct gaccgcggtg gccgccagaa gccggatagc ggcgacgatg
     2401 atcggagtta cgcccgcata gaacagcagg gaaaccagcc acgcaggacc actatcgccg
     2461 aagctgacgc gcaaagggaa taacggcgga tcgttgaaga tgtcatagat gttgaggtga
     2521 accgacttga cgctggtgcc gaagatggcg tcgagagcga acaccaggaa tcccaggaag
     2581 gccaagcgaa ccagacaggt aaccatgccg gcatagagcg caaaccagta tttggcccgg
     2641 ttgatgcggc cctcaaagct gaacaggaat tcgtctctgg tgtagctatt gcccatgacc
     2701 caacctccga accgcgagaa cgatctcgtt ttcggttatg cgccagccga tggaccggcc
     2761 cggtgcggaa caaattcgag ctcgcgctgc tggtcccagc gcggcctcgt atcgcgaggg
     2821 gccagcgggt cggacccaaa ccggttgggc ccgcgcgtcc ctttcaggca gcacatctcg
     2881 acaaaccccc atatgaaagc aatgaacacg gcaagcccaa tgaacactgc cgggtaggaa
     2941 ccgccaagcc gatctccaaa ttgaccgtag acaccggtag cagcgatgaa aggaacgatc
     3001 caccagccgc tcttgttgcg atcgtgaagc cgcttgatcg aggcggccgc gtaggaccac
     3061 ccgaagacaa gggtcatcgg aatgatgacg atttgcggaa acagcgaagc cttggatgca
     3121 gggtcgtcgt cgcctaactg gattgaagcg gaaatgccga tgacgttgat ggcaagcggg
     3181 ccgccaatgc cgaagatagt ggcaagggcg gccagtaaca tcagcacaaa aatcatcccg
     3241 cagagaatga tcaacgccgc aagccagtat ttggcgcggt tgatgcggcc ctcaaaactg
     3301 aacagaaacc atttgtagtc catcgcagcc tccggacggc cggaaatggc cgacggtccg
     3361 gagtattggt cgcatggacc ggctttgggt tcgaatccag agcgttttca agcgaagtgg
     3421 taccgttagt gttaagaaaa cgcgtcaaat tcaggaaatc ttgagtcccg tttcgattct
     3481 atcgaaacgg gactctagtc ccgcaacagc tcgttgatgc tggttttggc ccgggtgcgc
     3541 tcatcgacgc gcttgacgat cacggcgcag gcggtggacg gaccgggctg gccgttcttc
     3601 agcggccggg ccggcaagtt tcccggcacc accacggcgt attctggaac ttcgccgatg
     3661 aagatttcgc cgctgtcgcg atcgatgatt ttggtcgagg cgccgagaaa cacgcccatc
     3721 gccagcacgg cgcccttgcg cacgatcacg ccctcggcga cctccgagcg cgcgccgatg
     3781 aagcagtcgt cctcgacgat cacgggttcg gcctgcagcg gctcaagcac gccgccgatg
     3841 ccgacgccgc cggaaatatg cacgcgcttg ccgatctgcg cgcaggagcc gaccgtggac
     3901 caggtatcga tcatggtgct ctcatcgaca taggcgccga gattgacgaa ggagggcatc
     3961 agcacgacat ttttggcgat gaaggccgag cggcggacga tggcgcccgg cacggcgcga
     4021 aagcccgcgt cgcgaaaccg gttctcgccc cagccctcga atttcaacgg caccttgtcc
     4081 cacgacgatg cgttgccggg gccgccggga atcgcgctca tgtcgttgag gcgaaacgac
     4141 agcagcaccg ccttcttcag ccactggttg accttccatt tgccgccggc ctcgcgctcg
     4201 gcgacgcgcg cctcgccctt gtcgagcaac tcgagcgcat gatccacggc gtcacggact
     4261 tcgcccttgg tcgcggtgga gataccgtcg cgggcatcga acgcggtgtt gacggtggat
     4321 tcaagggaag cgagagacat cgaaggtttc cttggagatt tccttgggac gaattttgcc
     4381 tgcgcaggcg aggggctttg tcggaatttg gggcgggaga gtcaaggctc aacggctcgt
     4441 cgtcccggcg accgttcgac tcgtcattgc cgggcatgac ccggcaatcc atcttattcg
     4501 ctggacgctt tcgtagttga tggatgcccg gatcgagtcc gggcatgact gctgaatttc
     4561 cgacggggac caagcctagt ccctcgccac cccttccaaa aaccccgtca aatcatccgt
     4621 gacatgatcg acataagcgg cgtcgcggcc ttccagttcc cagtcctcgc gcacgacctc
     4681 ccgggtgccg tcgggcacca ccagcaccgt ggtcatgccg agctgatgcg gcaccacgag
     4741 gttgcgcgcc agatcctcga acatcgccga cgttcgggcg ttgaccccgt gaagtctgag
     4801 gaatttctga taggtctgcg gcgccggctt cggttcaagc tcggcggcga tgatatcgaa
     4861 caccgcctcg aaatgcgcgc cgatgccgag ccgcgccagt accttgtcgg catggtcggt
     4921 cgagccgttg gtcaggatca gcttgcgtcc gggcagtttt gcgatcgcca accccatctc
     4981 cgggttcggc tgcagcggcg aatgatcgat ctggtgcaca taagccagat agtcgtcggc
     5041 gtgcacgcca tgctcggtca tcatgccgcg catggtggtg ccgtagcggc ggtagtagtc
     5101 cttctggacc cggcgcgctt ccgccgcgga tatcttgagc caggcgctga cgaactcgcc
     5161 gatccgcaca tcgacctgct gccacagatt gacgtgatgc ggatacagcg tgttgtcgag
     5221 atcgaacacc caggtgtcga tgtggctgaa agcgcgggag gtgtttttca tcatcgtcat
     5281 cttaatccgt cgtctccgcg ttcgcgggga cgacgacggg aggatacatc atcacggcag
     5341 cgcgaaacgc aaagtcttgc cgccgctcga catgtcgacg gcggcaaagc cggtcacgcc
     5401 gagaccccgg gtgccgcaat cgccttgctc attgatctcg aacttgcttt cgcgcgtgca
     5461 cagctgggtg gtgccgcccc agttcaatgg cgtgtccttg accttgaggg cgcgattcgc
     5521 ggcgtcgacc gcctcggcga aagagaaaat ctgcttaggt gcgccggtga catcgggatg
     5581 caggcatttg ccgggctcga tgccatacca gccgcggctg atcacggtct tgccgtcgtc
     5641 ggtggcaacc gcggccatga tcttgtgcgg cgtgtcgttg caccaggtca gaccggtgga
     5701 agagggcgac tgcaccgcgt cgatcatcat cgtgaagaaa ttcggcgcct gcacgatatc
     5761 ggctgacagc ccccggcttt tcaggaacgc cgccaatgcg gcctgggtct tcggaccatc
     5821 gacgccatcg atcggcgcgg cgtcataacc tgcgatcacc agcagccgct gaatgccggc
     5881 gagccttgcc tgctcgtcat cgtattcgct gtcctcggca agataggcta cctgattgcc
     5941 gtcgtcggcc cgcgtcgggc tgatctgggt gaagggtgcc gggctttgcc cgctgcggca
     6001 ttgacgcgcg gcggcgatca cgaaatagtc gggcgcgatg caaagcgtat ccccgccgct
     6061 ctgcgggatc ggcgacgagc cgtagacgcc gagcgcgcgg gcggtcagca ggatgcggtc
     6121 ggcggtcagc gtgccctgcg cgacgacgcg gcacgccgcg ggatcgatgc gaaaccagcc
     6181 gcgcgtcgcg gtggcggact tgtcgtcgat gccgatcgcg gcctcgacca catagctcat
     6241 gcgattgcag agtttcaggt cggcgtaggc gggtacggcg gagaagagaa aggaaattac
     6301 cgcagcgggc aatgagatca gcgcagaacg caaggctgag taagaagcct gcgctgtacc
     6361 aagttcgtca tgccccggct tgtctcgggc atccacgtct ttcttgcgaa ctgggtgcaa
     6421 ggaagacgtg gatggccggg agcgcaggca agtatacata gtctgcgcaa ggcagactac
     6481 tatacccggc catgacgatg gatggtcatg cgtcatcact tgtgaatcag cgtgccggtg
     6541 ccttggttgg tgaataattc gagcaacacc gcgtgctggg tcttgccatc gatgatgacg
     6601 acgccctcga cgccctgctc gagcgcgtag atgcaggttt cgaccttggg gatcatgccg
     6661 ccggaaatcg tgccgtcggc gatcagcttg cgggcgtcct ttaccgacaa ttccggaatc
     6721 agcttcttcg acttgtcgag cacgccggga acgtcggtga gcagtaacaa ccgcttggcc
     6781 ttcagcgcgc cggcgaccgc gccggcaaag gtgtcggcgt tgacgttgaa ggtctgcccc
     6841 gacttcgcgg tcgccaatgg cgccagcacc gggatcagat catggccgat cagctggttc
     6901 agcagcgtca ggtcgacctt ttccggctcg ccgacgaaac cgagatcgac caccttttcg
     6961 atgttcgaat ccggatcgac catggtgcgc gtcaccttcg acgccgtcac catgttgccg
     7021 tccttgccgc acagcccgac cgccttgccg ccggcctcgt tgatgtagcc gaccagcgcc
     7081 ttgttgatcg aacccgccag caccatctcg acgatctcga tggtcgcggc atcggtgatg
     7141 cgcaggccgg cggcgaattc cgatttgatc ccgagccgct gcagcatctg cgcgatctgc
     7201 ggcccgccgc catgcaccac caccggattg atggcggtct gctccaacag aacgatgtcg
     7261 cgcgcgaacg ccttggcgag gtcctcggcg cccatcgcat ggccgccata tttgatgacg
     7321 atggtttcct cgtcatattg ctgcatgtgc ggcagcgcct cggacaggat gcgggcctga
     7381 tcgagcggac tgatatgggg cgcgtcggtc atgaagcggg tctcgtgggt cagcggggtg
     7441 gggcgggttc tatccgattg gcggccacgg cgcaaagctt cgctgccttc ttcccctcgc
     7501 cccttgtggg agagggtggc acgggcgcgc gaagcgcgtt cgtgacgggt gaggggttct
     7561 ctccgcggat cgcggataaa gacccctcat ccggcgcgct tcgcgcgcca tcttctccca
     7621 caagggaaga aggaagaaag tgccgcctca gccccgccgc ggccatgccg cggcgaccgc
     7681 gagcaccagc cagctgacga tcagaagcgt gccgccggtc ggcgcggcca tcgaaaacag
     7741 gctttggccg gcatattgcc gcagcgtcag atcgccggcg aacagggccg ccgcgatgac
     7801 aaagccgaat gcggcggcga tgcctattcg gacatggatg acgccgcggt cggccagcgc
     7861 caccgcggcg agcacggcgg tagcatggaa cagcagcatc gatgaggccg acgccagccg
     7921 cgaggcatcg gcctgatgcg cggaggctgc ggcgaggacg acgccgtcgg cgcccatgat
     7981 cccggccaag acgatcagaa tgcggaacag gcgggatggc atcatcggct gcgctcgccg
     8041 agcaggcgca ccatggctgc gcgcaattcc ggcattccgg cgccggtgcg cgacgatgtc
     8101 accaaaagct ccgggaaggc ggcaggatgt ttggcaagtg ccgtctgaac ctccgcggcg
     8161 cgctgctcga gttcggccgg cttcacctga tcggccttgg tgaggaccac ctgatagctg
     8221 acggccgatt tgtcgagcgt cttcaggacg tcgagatcga cctccttgaa gccatgcctg
     8281 gcgtcgatca gcacatagac ccgcgccaga ctgctgcgac cctgcaggaa tgtgtggatc
     8341 agcgcggtcc atgacgcgac cttggctttc ggcgcggagg cgtagccgta gccgggcata
     8401 tcgaccagcc ggaagccggc attgtcaggg ccttcgaaga agatcaattc ctgggtgcgg
     8461 cccggagtgt gggaggtgcg cgccagcgcg ttgcgcccgg tcagcgcgtt gatcaggctc
     8521 gatttgccga cattggagcg gccggcgaac gcgacctcca tgccggccat cggcggcagc
     8581 gtttcgatcg agggcgaggc ccaaaagaac tgccagtcgc cggcgaacaa ttttcgccct
     8641 tgttcgatca gcttcgcatc gatgtcgggg ggcatgcgaa gggctttcat catcgcggcc
     8701 aagccgtcat tgcgagcgaa gcgaagcaat ccatcttacc gcgaagaaag catggattgc
     8761 ttcgtcgctt cgcttctcgc aatgacggaa agcaagtccg aggcgtcctg gtctaggctt
     8821 taaccgttcg tcaggttttc gactcgacct tcttcgaggc aaacgtcgcc ctgagattgt
     8881 cgaacaattc aaccttcacg ccattgcggc gcatgatgaa gctctgctgc agcaccgaca
     8941 gggtgttgtt ccaggcccag tagatcacga gaccggcggg gaagcccgcg agcatgaagg
     9001 tgaagatcag cggcatccag tcgaagatca tcttctgggt cgggtccggt ggcgtcggat
     9061 tgagcttcat ctggaaccac atcgtgatgc ccatgatgat cggccagacg ccgagcgcga
     9121 ggtagtgtcc gaacagcggc agggtggtcg gatcgaagtg cagcaggccg aacaggttga
     9181 acaggttggt cggatcgggc gccgacagat ccttgaccca gccgaagaac ggcgcatgcc
     9241 gcatttcgat ggtgacgaac agcaccttgt agagcgagaa gaacaccgga atctgcaagg
     9301 cgacgggaag acaaccggcg atcgggttga tcttctcctt cttgtagatt tccatcatct
     9361 cctgctgctg cttcaccttg
//
LOCUS       DATNOI010000117        19836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1717745,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000117 DATNOI010000000
VERSION     DATNOI010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 19836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19836
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717745"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VN968_04275"
     CDS             complement(<1..1230)
                     /locus_tag="VN968_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXL28490.1"
                     /translation="MDQRDVRAAWEKFVERGALSADLRSSVAASWQRSRNHRVTVDRA
                     RAPLVADAELFRHRSKHASLRHAARCALENSKTFLGDANSIMILTDPTGLIIDTQGDD
                     RVIDAGRTVHLEHGGRWSEADIGTNAIGAAIAESKPVQIRGAEHFCSEVQRWTCAAVP
                     VHDPTDGELLGVVDISGPASTFNPQSLALAVSVGHHVESVLAQSVRRDHEELLCHFLA
                     KRSLWVNEEFIVLDRRGMILHATERALTALHNNRHGIDGDAPTRFLKTLPFDEWPVKL
                     KELLPNASFDLVENETSGIGAIMVMHARRRPIADRDIEKPKPAFERRGTASSSHPRKD
                     LAPGPQHLQDKVGSTTSRFVARDPTVRAIVRQVETAATRKMPILIRGETGTGKEQLAR
                     HAHAASGRTGAFVPVNCA"
     gene            1246..1683
                     /locus_tag="VN968_04280"
     CDS             1246..1683
                     /locus_tag="VN968_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28491.1"
                     /translation="MTKSETRIQTGLAINHLKCGVLAAITVAIMQDATSNPFQPPKRW
                     VPRIESKYRRYCHDDYGVIQPNIPSSVYFPDIPCGSSAPLISTTYTSWLIGAKEESKD
                     PELLASSSVVTEEAEGSGLNSKMVASPGIAACEGLAAAQLGRR"
     gene            complement(1746..3329)
                     /locus_tag="VN968_04285"
     CDS             complement(1746..3329)
                     /locus_tag="VN968_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL28492.1"
                     /translation="MSASNVPLLEVFGIVKRYGGLVAIDHIDLQLQPGTITGVIGPNG
                     AGKSTLIGLIGGAVVPSEGSIRLDGQDISRLPASERARAGIGRTYQIPRPFLDMTVEE
                     NLEVAQYSIQPFIRAAVARDERAALLKRTGLVDAASLPARALPLLRRKRLEVARALAL
                     KPRIVLLDEVGAGLVDSEITELIALIHSIADPRTAIVIVEHVLRVVRECCARSMVLNF
                     GKKLVEGPTMEIFANDEVAAVYLGTGGSREPSVSPARVIDAEAQDAVHIVGPEAERSV
                     MSSLIALPPVVEAKIPLLDLQGICSGYGQARVLKGIDLTICEGQAVAILGANGAGKTT
                     LARTISGAITPTSGVLRIAGEDVLGRPPHRIAELGIAHCMEGRRIFSTLTVQENLLIA
                     ARHADASAARERLGAVYDLFPVLGERKDQGGTSMSGGQQQMLAIGRALMARPRLIIFD
                     EISLGLAPVVMDKLYDALAVLKQAGMTLIIIEQDVDRALALADHAYVLEHGVFGLSGA
                     PGQIAADPRLRHIYIGTAD"
     gene            complement(3326..4264)
                     /locus_tag="VN968_04290"
     CDS             complement(3326..4264)
                     /locus_tag="VN968_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL28493.1"
                     /translation="MKTLSGLAEAGVLLGAFILLWFASDFVDDYGLQIAFRVLIYLTL
                     AESWNLLAGSVGLVSLGTSCFVGLGAYVAFGLLNNIDAPLPLVLLGCALAGGALAALV
                     SPALFRLRGLYFTVGTLALAELLRLTMVNVSAFGGAMGIFMQKDPPPLPELYHYALGV
                     ALIASLAITACARTRVSVILRAVRDDEDAAAQMGVRPYRVKLGAFVVASALVAIAGGV
                     QATKLGAIEPYGMFGLQWSIDVLSMVIIGGVGLRLGPLVGALFVVALGEALADYPEAH
                     VAIAGLILIVLIRFAPGGVVGFAETLSSPTRKRATA"
     gene            complement(4261..5136)
                     /locus_tag="VN968_04295"
     CDS             complement(4261..5136)
                     /locus_tag="VN968_04295"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL28494.1"
                     /translation="MSDVGTVLSTIISGMLLGGMLALTALGLSIVLGVMRLVNLAHGD
                     FLVVGAYAGLFFLKFTGIDPLLGLPVIALLVAALAVPLYGLLLAPLGERGAEAQMMTM
                     FAVSIILENVFVLAFSADTRSIERDYATLPLTLGSVTVPLIYVIGFAIAVSLILAVHW
                     LISKTRFGRDLRASAADAQAAETLGVDVKRVQMATFALGAACAGVGGTLIGAAFQFGP
                     SSGAAYLLNSLAIVVLGGLGNVLGTLVGGLMLGFLQSLGGLTLGDGYRDLVGMALFLL
                     VLAFRPDGFLARRRS"
     gene            complement(5155..6348)
                     /locus_tag="VN968_04300"
     CDS             complement(5155..6348)
                     /locus_tag="VN968_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL28495.1"
                     /translation="MGNLKSIFRAGAIAAGASIALGLGGARADEIVVGASLPLSGALA
                     GFGSFQQWGYKRAFDEVNKAGGISIGGTRQMVKLIIRDDKTDPNATASNTETLISRDK
                     VVAMLGSCTPALVNAGALVAERAKKPMVTGCDPLEVFMSVRKWNYVWDIFFSVPELAA
                     AQFKALTDLGVQTNKKIAIWHDNGPDGQAVGGKIWPALAKEFGYEVVQNAEFPVDNTQ
                     FTSIIAEAKSKGAEVVLGDAITPQAIAIRKQMASAGYTPKILNIEKGAEPVQFAEALG
                     KISDGIFVGGYWDPSFPYPGAADLAKAFESETKLTSSQHIADSYAAAQVLLDAIKAAA
                     STDPEKINAAIAKTDKTYVVGPVKFDENHTAKLPIVSLQWQGGTTVIVWPKSAKTGEL
                     LFPLP"
     gene            complement(6422..7084)
                     /locus_tag="VN968_04305"
     CDS             complement(6422..7084)
                     /locus_tag="VN968_04305"
                     /inference="COORDINATES: protein motif:HMM:NF036707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28496.1"
                     /translation="MRFEDANEMLKPGYQRFESGYQELEDGKWVVAGLTRMPGCRAKM
                     VDWWFGWLGDIEWYRLWHPRDHVYSGWENRVNGRYIGASHLVHEYLAGKDGPLFKLRI
                     DFHDPSETFDAKLYKESGALAVCARPGLLEAPFHVGRMTHYVRDTDYGCEMRSRFWLG
                     LISHRDPTITLPEAQVRAMRAQNLNAEFARRLHQHCVEEMGYLAEILPLLYRRVTLDD
                     AF"
     gene            complement(7160..8095)
                     /locus_tag="VN968_04310"
     CDS             complement(7160..8095)
                     /locus_tag="VN968_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXL28497.1"
                     /translation="MTEKPVMAVIGAGLMGAGIALVFAAAGHRVALFDAMAEARATAL
                     PRIRDNLKAIALDPVAAARVTLHAQLATAVADVDFVIEAAPEDLALKQELFAEIEDAA
                     KPAAVLASNTSVIPITSIIQRLRNKARALGTHWWNPPYLVPLVEVIRTVDTSDAAVGA
                     TLKLLASVGKTAVEVKKDVPGFIGNRLQHALWREAVALVADGVCDAKTVDDVVKASFG
                     TRLAVLGPLENADLVGTDLSRSIHNYVLPALDRSTAASPYLDRPVEQGRLGFKSGEGF
                     RKWTPEQQAALRERVTNHLRASFGAPVSAGSDDVG"
     gene            complement(8100..8900)
                     /locus_tag="VN968_04315"
     CDS             complement(8100..8900)
                     /locus_tag="VN968_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL28498.1"
                     /translation="MTLGLANPLSLFDVKGKVAIITGASGAFGALAAEVLAAAGAKLV
                     LAAGKARELEDIAVSCRKLGADVEPINKRPSSEAECESIVEAAKRRFGRVDILVVASG
                     KNDVSKIVEMAPERFLDVMDANVTQSWLMARSVSKQMLAQGEGGKIVLMSSARGLLGH
                     PAGYTAYCASKAAIDGITRALGCELGPTGITVNAIAPTVFRSPLTAWMFADDEKAKAV
                     RAGFLARVPKGRLGEPEDLAGPLLFLASRASDFYTGHTLYADGGYTAG"
     gene            complement(8897..9247)
                     /locus_tag="VN968_04320"
     CDS             complement(8897..9247)
                     /locus_tag="VN968_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL28499.1"
                     /translation="MHVKRYEDAKPYDAPNHRGVVGLRLQGFEPGGPTNQWIGYSLFL
                     PGGGAGPDSTPFEKVYVVLEGHMTVIIGGTETVLGPMDSCTIAPGEVREIINRTNSVC
                     KMLVVIPYPPGVKP"
     gene            complement(9311..10150)
                     /locus_tag="VN968_04325"
     CDS             complement(9311..10150)
                     /locus_tag="VN968_04325"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXL28500.1"
                     /translation="MVKIRGIEFQANTDNPMGLEFYNLSHRFGFQSPNWPYFEDVKIE
                     RIHYMAKSGVLSQRITTSMHNTTHIDAPAHVVEDTPFIDEVPLPHFFGSGLVVSIPKE
                     MWEPITYDDLDKAAGKAVRPGDVIIVNTGWHHKYRDSEEYFCKCPGFVPSAGDWFVEK
                     KIKVVGHDTQANDHPLATAIGPHRNGPLHPHLAEEFKKWSGGIDWKQAHPEWEPVHRK
                     LFTNGILGIENVGGDLDAVTGKRVTFAFFPWNWDRGDGCIIRLVAMIDKSQNYRIANG
                     AAY"
     gene            complement(10300..11070)
                     /locus_tag="VN968_04330"
     CDS             complement(10300..11070)
                     /locus_tag="VN968_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HXL28501.1"
                     /translation="MKTIETNEKSQVQVIARAAAILRALEHENAGLSLGQIAQRVDLA
                     RSTVQRIIAALEVEKLVIAASPNGKMRLGPTILRLAASVRTDFASLARPFLVALSKEL
                     GETVDLSIIKKDQAVFIDQVTGPHRLRTVSVVGDAYPLHCTANGKAYLGRIDDDAIVD
                     LIGRKYARRTKHTKVTFAELVADLRVTRQSGVAVDLEEHSSGVCATGVALRDPAGNYV
                     AISVPVPSQRFKDEYKTIARRLLETKAVLEKLIISRNL"
     gene            11344..12294
                     /locus_tag="VN968_04335"
     CDS             11344..12294
                     /locus_tag="VN968_04335"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL28502.1"
                     /translation="MVGKIALEEHFLCPGFEAYWAATVSGVAPDALQRITARLSDFGD
                     GRIEAMDKAGIAKAVLSLAGPGVQAEPDRATAVRMAAGANDYLARELQKGRDRYAGFG
                     HLAMQDANSAADELERCMRDLGFSGAMINGHTNGQYLDHPSLAPFWERAAALRAPIYI
                     HPTDPVTPSPVLDGYQALRRATWEWGFETGSHALRLVFGGVFARFPDAKVILGHLGET
                     LPYLLWRFDSRARLYGVELARPPSSYIKSNIFVTTSGMFSAEPLACAIAALGHDRVMF
                     SADYPFEDPEEAGSFMDRVALPEDVRADIASNNARRILKL"
     gene            12355..13365
                     /locus_tag="VN968_04340"
     CDS             12355..13365
                     /locus_tag="VN968_04340"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXL28503.1"
                     /translation="MTISISRRAFALTGACAVITSRSASAQTAELKVSHFLPPNHTIH
                     KALVMWSDDLEKQSAGRLKLRIYPAGQLGGGPNRQFDAARNGVVDMAFSLHGATPGRY
                     PMTELASLPFAAPSAGNKSMVTSRRLSELAPEYLAKEHEGLRILWMAVTPPLMFHSKT
                     PIRTIADFKGKRIRYAGVQFKNIIDSLGAVPLLVPPQETQDGLAKGIIDAATFPYEGA
                     ASFDIATVAKYTLEPGVSSATFALVMNPVKYASFPDDLKALIDKTTGPEAATGYGAMW
                     DEAENEGKASMLSKGAQVTVLADDQLEQMKTILRPQRDAAVADVESQGKPGRKFLDVY
                     LK"
     gene            13443..13958
                     /locus_tag="VN968_04345"
     CDS             13443..13958
                     /locus_tag="VN968_04345"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXL28504.1"
                     /translation="MTSQASSPSTPPPAVKRLQTAQLRLAAVALMTMMLGTALDVFFR
                     YAFNKPLRISYDLVESMLVIYVFHGISSVFLARRNIVIDVIDSLVGPRILRGLAVIAD
                     VVSVLCLLLFLWGMYEPALQAYQYGDRKLELGEPLYVLWAFALAGLLGTLVCAIAAFT
                     HITLRQFRRHS"
     gene            13955..15259
                     /locus_tag="VN968_04350"
     CDS             13955..15259
                     /locus_tag="VN968_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXL28505.1"
                     /translation="MSPAIIGALGISSLLVLLFVRVPVWAALTLVGFTGNFTLSGLHS
                     ALSLAGTSAFDMSSAYTLSVIPLFVLLGEVASNTRLSADLFNAARLLLSGVRGGLAIA
                     TIAASSCFGAVCGSSIVTAATMTRIALPEMRKAGYDDRLATGALAAGGSIGILIPPSI
                     ILVIYASIAEQSVPKLFAAALLPGLLLTALNMTVAIGIAIVYPHYAPRMEKASARERF
                     KALAGPWQFILLFVITIGGIYAGIFSPTEAAAIGAFGSIVLGFLGRRLTWATLWRSIE
                     TSITTSCLLFVIIIGANLFSSFMVQTQFPTMLADGASAMNLSAPVVMIMIVLAYIVMG
                     CFLEGIGMVLITVPVFLPLVVKFGYDPIWFSILVVIVVEVGLIHPPVGMNLFVIQAQA
                     PDVKIASIYRGIVPFLIAPLVLIIFLFLFPSLALWLPKLLYG"
     gene            15266..16222
                     /locus_tag="VN968_04355"
     CDS             15266..16222
                     /locus_tag="VN968_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HXL28506.1"
                     /translation="MTNVLVLETHAEAYAAALREEFPDLELQVARTPADAHVDYGKVE
                     ILIAFGMDVTDLLFTGMPGLKWVQSLATGVDHFLRSKTLARDIILTSGRGIHGPAMRE
                     TVLHLMLSISHDSNGLVRDKVGRRWDRRAWPLLAGKTAAVIGTGVAGSAIGSLLQALE
                     MRVVGVSRTPRQVAGFDTVVPQSELSEAAAKADYVINVLPANAVNLKIIGSDVFVRMK
                     PGAYFIDVGRGETVDEEALIEVLRDGKIAGAGLDVFATEPLPAQSPLWDLPNVFMSPH
                     IGGLFEEYQQMAMPLIIRNMSAYLSGRTDQMTNVIDRSRSVT"
     gene            16219..17424
                     /locus_tag="VN968_04360"
     CDS             16219..17424
                     /locus_tag="VN968_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HXL28507.1"
                     /translation="MTGMIDPASRILTRISETELNRRWNAVRQAMKQRGIDAVLMQNT
                     NDWLGGYVKWFTDLPANNGYPRTVVFHADAPMSVVEMGPSNIRRTFASDETLHRGVGE
                     MLHTPSFTSIGYTDRDDVHLAIDALRRHSNRTLGLITPGALPYAFVSALKAAFAATTF
                     IDVTSDIDAIKAIKSEEEVALIRQTARLQDEAFAEVLKEIRPGLRDIDVTAIAQRKGQ
                     LLGSEQGIFLGGSAPVGIRSPFVPRYMQGRRLEKGDHLSLLIEINGPGGFYTEIARTI
                     VLGKASAELKEAFASVKAAQDHSLSIMKPGTQVRDVFASHNAYMASNGMPAETRLYAH
                     GQGYDMVERPLIRQDDDMTLQQGMCLAVHPGYETPSLFAVICDNYVIEADGPGGCLHH
                     TEKRLFEID"
     gene            complement(17477..17857)
                     /locus_tag="VN968_04365"
     CDS             complement(17477..17857)
                     /locus_tag="VN968_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28508.1"
                     /translation="MRYGPFILGLLVLASSAHAESRSAYVTMVLQAFAAKVECPGMDI
                     VYQDLVQKAQEMHLPDGTTEKVRKAIAYMHTGGKMGERQDDDLMAEVALATKSTDLDQ
                     TRLGMTSWCETQKTRLAGFIRTKN"
     gene            18320..19294
                     /locus_tag="VN968_04370"
     CDS             18320..19294
                     /locus_tag="VN968_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL28509.1"
                     /translation="MKLAANLFAALLVLLAAQTASAQSSYPNRPVKILVGFTPGTAPD
                     LSARILADQFSEVWGTPFVVENIPGAGSNIATERVAKAAADGYTLLMGGNSALVINPS
                     LYETLPFDPLKDFEPISQVFIAANVLAVPPELPVKSVAELVALAKAEPGKLSYGHAGV
                     GTSQHLGAELFKFMAHVDIAPVPYRGTTALMPDLLANRINMSFANIVNVVPLAREGKL
                     RALAITSIKRSALAPDLPTMAESGFPGFEAVPWFGLLAPIGTPKDVLDKLHGETVKAL
                     AMPEVRKKFDELGLEPVGDTAAEFAAIIKKETPEWAKVIKDAGIKLGN"
     gene            complement(19647..>19836)
                     /locus_tag="VN968_04375"
     CDS             complement(19647..>19836)
                     /locus_tag="VN968_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arabinose ABC transporter permease"
                     /protein_id="HXL28510.1"
                     /translation="GTASGFAYMFVKLPSFLAIFLFPSLFAAIGQANATLFVAIFPLI
                     GLLAAVFILPEVYGYEHD"
BASE COUNT         3765 a         6103 c         6151 g         3817 t
ORIGIN      
        1 ggcacaattg acgggaacga aggcgccggt ccgcccgctg gcggcatggg cgtggcgggc
       61 gagttgttcc ttgccggtac cagtctcacc gcggatgagg atcggcatct tgcgtgttgc
      121 cgcagtctcg acctgacgga caatggctcg caccgtcggg tcgcgggcga cgaagcgcga
      181 tgtcgtgctg ccgaccttgt cctgaagatg ctgtggtccc ggcgccagat ctttgcgagg
      241 gtgcgacgaa ctcgcggtcc cgcggcgttc aaatgcgggc ttcggtttct caatgtcgcg
      301 atcggcgatt gggcgccgcc gggcatgcat caccatgatg gcgccgatgc cggaggtttc
      361 gttctcgacg agatcgaagc tcgcgttcgg aagcagttcc ttcaatttga ccggccactc
      421 gtcgaagggg agcgtcttta aaaagcgggt tggcgcatca ccgtcaatgc cgtgacggtt
      481 attgtggaga gcagtcaggg cgcgctcggt ggcgtggagg atcatgcctc gacggtcgag
      541 cacgatgaac tcctcgttca cccacagcga cctcttggcg agaaagtggc acagcagctc
      601 ttcgtgatct cgtcggaccg attgcgccag gacactttcc acgtgatgac cgacagagac
      661 ggccaaagcg aggctctgcg gattgaaggt gcttgccggg cccgagatgt ccaccacgcc
      721 gagcagttcg ccatcggtag gatcgtgaac cggaacagca gcacaggtcc atcgctgcac
      781 ctccgagcaa aaatgctccg cgccgcggat ttggacaggt ttcgattccg ctatcgcagc
      841 gccgatcgcg ttggtgccga tgtcggcctc gctccagcgc cctccatgtt ccagatgaac
      901 cgtgcgacca gcgtcaatga ccctgtcatc gccctgggta tcgatgatca atccggtggg
      961 atcggtgagg atcatgattg aattcgcgtc gcccaggaat gttttcgaat tctcgagcgc
     1021 acatcgggcg gcatggcgca gtgaagcgtg cttggagcgg tgacgaaaca gttcagcgtc
     1081 tgcaacaagc ggcgcccgcg cgcggtcgac cgtgacccga tgatttctgg atctctgcca
     1141 cgatgcagcg accgacgatc tcaaatcggc cgataacgct ccgcgctcga cgaatttctc
     1201 ccacgcggcc cgcacgtctc gttggtccat cccggctcct ccgacgtgac gaagtccgaa
     1261 acccgcatac aaacgggcct cgcgatcaat catttgaaat gcggggtgct tgccgcaatc
     1321 accgttgcca tcatgcaaga tgccacttca aaccctttcc agccaccgaa acggtgggtt
     1381 cctcggatcg agtcgaaata caggcgctat tgtcatgatg actacggcgt catccaacca
     1441 aacatcccat ccagcgttta cttcccagac atcccatgcg gttcttcggc gccgctgatc
     1501 agcacaacct atacttcttg gctaattggc gcaaaggagg agtccaaaga tccggagctt
     1561 ttggcgagct catcggttgt cacggaagag gccgagggat ccggtttgaa ctccaaaatg
     1621 gtcgcctcac cgggaatcgc agcttgcgaa gggctcgctg ccgctcaact tggtcgaaga
     1681 taggtgcagc gcagcatcct cgaaatgact tcaagctttc ggtgagccca ctttgtcgcg
     1741 gtcggctagt cagcggttcc gatatagata tgacgcaagc gcgggtcggc ggctatttgg
     1801 cctggcgcac cggaaaggcc gaacacgccg tgctccagga cgtaggcatg gtcggcgagc
     1861 gccaaggcgc gatcgacgtc ttgctcgatg atgatcagcg tcatgccagc ctgtttgaga
     1921 accgcaagtg cgtcgtagag cttgtccatg acgaccgggg caaggccgag gctgatttcg
     1981 tcaaaaatga tcagccgcgg gcgggccatc agggcgcgac cgatcgcgag catttgttgc
     2041 tgaccgccgg acattgaggt gccgccctgg tcctttcgct cgccaagcac cgggaacagg
     2101 tcatagacgg cgccaagccg ctcacgtgcg gccgaagcat ccgcatggcg tgccgcgatc
     2161 agcaagtttt cctgtaccgt gagcgtcgag aaaatgcgcc gaccctccat gcaatgggca
     2221 attccaagtt cggcgatgcg atgcggtggc cggcccagga cgtcttcgcc ggcgatccgc
     2281 aacacgcctg aagttggtgt tatggcgccg ctgattgtcc gtgcaagcgt tgtcttaccg
     2341 gcgccgttgg cgcccaggat ggcgacggct tgtccttcgc agatcgtcag gtcgatgccc
     2401 ttgagcacgc gggcctggcc gtagccggag catattcctt gcagatcaag cagaggaatc
     2461 ttggcctcaa cgacgggtgg cagggcaatc agggacgaca tcacgctgcg ttcggcttcg
     2521 gggccgacga tatgcaccgc gtcttgcgcc tctgcgtcga tgacgcgtgc tgggctcacg
     2581 gatggttcgc gggaaccgcc ggtgccgaga tagaccgccg caacctcgtc attggcaaag
     2641 atttccatcg ttgggccttc gaccaacttc ttgccaaagt tgagcaccat cgagcgcgcg
     2701 cagcattcgc gtacgacacg gaggacatgc tcgacgatga caattgccgt gcgcgggtcc
     2761 gctatcgagt ggatgagcgc gatcagttca gtgatttcgc tgtcgaccag gcccgcaccg
     2821 acctcgtcga gcaagacgat gcgtggcttg agcgccagcg cacgggcgac ctccagtcgc
     2881 tttcgccgaa gcagcggcag ggcgcgggcc ggcaggcttg cggcgtcgac gaggccggta
     2941 cgcttcagaa gcgccgcgcg ctcgtcccgc gcaacagcag cccggatgaa gggttgaatt
     3001 gaatattgcg cgacttcaag attttcctcg acggtcatgt ccaggaaggg gcgggggatt
     3061 tgataggtgc ggccgatgcc ggcgcgggcg cgctcggacg ccggaaggcg cgatatgtcc
     3121 tggccgtcga gcctgatcga accttcgctt gggacaaccg cgcccccgat caggccgatc
     3181 aaggtcgatt ttcccgcgcc gttcgggccg atcacgccgg taatcgttcc cggctgcaat
     3241 tggagatcga tgtgatcgat cgccaccagg ccgccgtagc gcttgacgat gccaaagact
     3301 tcgagaagcg ggacgttgct cgcgctcatg cggttgcccg cttcctggtc ggggaagaca
     3361 gagtttccgc gaaaccgacg acacccccgg gcgcgaatcg tatcaacacg atcaggatca
     3421 ggcccgcgat cgcgacatgc gcctctgggt agtcggccag cgcttccccc agcgcgacga
     3481 cgaacaacgc gccgaccagg ggacccaatc gcaggccgac gccgccgatg atcaccatcg
     3541 aaaggacgtc gatcgaccat tgcaatccga acatgccata gggctcgatg gcgccaagct
     3601 tcgtcgcctg caccccgccc gcgatcgcaa ccagggcgct tgcaacaacg aaggcgccga
     3661 gcttgacgcg gtacggacgc acgcccattt gggctgcggc atcttcgtcg tcgcgaaccg
     3721 cgcgcaggat cacggaaaca cgggtgcgcg cgcaggccgt gatggcgaga gatgcgatca
     3781 gcgcgacgcc cagggcgtaa tgatagagtt caggcagggg aggcggatct ttttgcatga
     3841 agattcccat cgccccgccg aacgcagaga cgttgaccat ggtcagccgc agcagttcgg
     3901 cgagtgcgag cgtaccgacg gtgaaataaa ggcctctcag acgaaacagc gccggcgaaa
     3961 ccaacgccgc caatgcacca ccggccagcg cacagccaag gagaaccagc gggagtggcg
     4021 cgtcaatgtt gtttaacagc ccgaaggcaa cataggcgcc gagcccgaca aaacacgacg
     4081 tgccgagcga aacgaggccc accgaccccg ccagcaggtt ccagctttcg gcaagcgtca
     4141 aatagatcag gacacggaag gcgatctgca acccgtagtc gtcgacgaaa tcggacgcga
     4201 accagagcag gatgaatgcg ccaaggagga cgccggcctc tgcaagcccc gacagcgttt
     4261 tcatgatcgc ctccgcgcga gaaagccgtc aggtcgaaaa gccagcacca gcaggaacag
     4321 cgccatgcca acaaggtcac ggtaaccgtc gccgagcgtc aggccaccga ggctttgcag
     4381 gaagccgagc atcaatccgc caacgagcgt acccagcacg ttgccaaggc ccccgagcac
     4441 gacgatggcg aggctgttga ggaggtaggc ggcgccgctc gaagggccaa actggaacgc
     4501 ggcgccgatg agcgtgcctc cgacgcccgc gcaggccgcg ccgagcgcga aggtggccat
     4561 ctgcacgcgt ttgacgtcga cgccgagcgt ttcggcggcc tgagcgtcgg cggcgctcgc
     4621 gcgcagatcg cgtccgaagc gggttttcga aatcagccag tggacggcca ggatcaacga
     4681 gacggcgatc gcaaagccga tgacatagat cagcggtacg gtgaccgacc cgagcgtcag
     4741 ggggagcgtt gcgtaatcgc gctcgatcga gcgtgtgtcg gcgctgaagg caaggacgaa
     4801 gacgttttca agaatgatcg aaaccgcaaa catcgtcatc atctgcgcct cggcgccgcg
     4861 ttcaccgagc ggcgcgagca gtaatccata gagcggaacc gcgagagctg cgacgaggag
     4921 ggcgatcacg ggcagaccga gaagtggatc gatgcccgtg aacttgagaa aaaacaggcc
     4981 ggcgtaggcg ccgaccacca ggaaatcgcc atgggcgaga ttgacaagcc gcatgacgcc
     5041 caggacgatg gagagtccaa gggcagtcag cgcgagcatc cctcccagca gcatgcccga
     5101 aatgatcgtg ctcaacacag tgccgacgtc ggacatgaag ctgggcccgt cagatcaggg
     5161 aagcgggaac aggagttcac cggtcttggc cgacttgggc cagacgatca cggtcgtgcc
     5221 gccctgccat tgcaacgaga cgatcggcag cttggcggtg tggttctcgt cgaacttgac
     5281 gggtccaacc acgtaggtct tgtccgtctt ggcgatggcg gcattgatct tctccggatc
     5341 ggtcgaagcc gcggccttga tggcgtcgag caggacctgc gcggcggcat aggaatccgc
     5401 gatgtgctgg ctgcttgtca gcttggtctc gctttcaaag gccttggcga ggtcggctgc
     5461 gcctgggtaa gggaaggaag gatcccaata gccgccgaca aaaatgccgt ccgagatctt
     5521 gccgagcgcc tcggcgaatt gcacgggctc cgcgcccttt tcgatattga ggattttcgg
     5581 cgtgtagccc gcggaggcca tctgcttgcg gatcgcaatc gcctgcggcg tgatcgcgtc
     5641 accgagcacg acctcggcac ccttgctctt ggcttcagcg atgatcgagg tgaactgcgt
     5701 gttgtccacc gggaattcgg cgttctgaac gacctcgtag ccgaattcct tggcgagcgc
     5761 cggccagatc ttgccgccga cggcctgacc atcgggaccg ttgtcgtgcc agatcgcgat
     5821 tttcttgttg gtttgcacgc ccagatcggt gagcgccttg aactgagccg ccgcgagttc
     5881 gggcaccgag aagaagatat cccagacata attccactta cgaaccgaca tgaagacctc
     5941 gaggggatcg caaccggtca ccatcggctt cttggcgcgt tcggcgacaa gtgcgccggc
     6001 gttcaccagc gccggcgtac aggagcccag catggccacg accttgtctc gggagatcag
     6061 cgtctcggta ttggaggccg tcgcgttggg atcggtcttg tcgtcgcgga taatgagttt
     6121 caccatctgc ctggtgccgc cgatcgaaat accgccggcc ttgttcactt catcgaacgc
     6181 gcgcttgtaa ccccattgct ggaagcttcc gaaacccgca agcgcgcccg acagcggcaa
     6241 cgaggcgccg acgacgattt cgtcggcccg ggcgcccccg aggccgaggg ctatggaagc
     6301 gccggcggcg atcgctccgg ctctgaagat gcttttaaga ttccccatat gtatgttcct
     6361 ccctttgttt ttgttcggcg gatctggttg tttctgcacg tttggtctcg aagaggtccg
     6421 gctagaaagc gtcgtcgagc gtcacgcggc gatagagcag gggaaggatc tcggcgagat
     6481 atcccatttc ctcgacgcaa tgctgatgca ggcggcgcgc gaattctgcg ttcaggttct
     6541 gcgcgcgcat ggcgcgcacc tgggcctccg gtaacgtgat cgtgggatcg cggtgcgata
     6601 tcaggccgag ccagaagcgc gagcgcatct cgcatccgta atcggtgtcg cgcacgtaat
     6661 gggtcattcg cccgacatgg aacggcgctt cgagcaggcc aggacgggcg caaaccgcga
     6721 gggcgccgga ctccttgtaa agcttggcgt caaaggtttc cgaagggtcg tggaaatcga
     6781 tgcgaagttt aaagagcggc ccgtctttgc ccgcgagata ctcatgaacg agatgagaag
     6841 cgccgatata tctgccgttc acccggtttt cccagccgct gtagacatgg tcacgcgggt
     6901 gccacaggcg ataccactcg atgtccccga gccagccgaa ccaccaatcg accatttttg
     6961 cccggcaccc gggcatgcgg gtgaggcccg cgacgaccca cttgccatcc tcaagctcct
     7021 gatagccgga ctcgaagcgc tggtagcccg gcttcaacat ctcgttcgca tcttcgaacc
     7081 gcatgtactt tcctccggct tgctttgatc ggcggtctag aatcccactt gatcgccgcc
     7141 tttcttgttc ggcttaggtt tagccaacgt cgtcactccc agccgagacc ggcgcaccaa
     7201 agctcgcccg caaatgattt gtcacgcgct cacgcaaggc cgcctgctgc tccggcgtcc
     7261 atttgcgaaa cccttcgccg gatttgaatc cgagccggcc ttgttcgacc ggccgatcga
     7321 gatagggcga agccgcggtc gagcgatcga gcgccggcag cacataatta tggatcgacc
     7381 tgctaagatc agtgccgacg agatcggcat tctccagcgg gcccagcact gccaaacggg
     7441 tgccgaaact cgccttgacg acgtcgtcga cggtcttcgc gtcgcagacg ccatcggcga
     7501 ccagagcaac tgcctcgcgc cacagcgcat gttgcagtcg gttgccgata aaacctggca
     7561 cgtccttctt cacttcgacg gcggtttttc cgaccgaggc tagcagcttc agcgttgcgc
     7621 cgacggcggc gtccgacgtg tcgaccgtgc gtatgacctc gacgaggggc accagatagg
     7681 gcgggttcca ccagtgcgtt ccgagcgcgc gcgccttgtt gcgcagcctt tggatgatcg
     7741 aggtgatcgg gatcacggag gtgttggagg ccagaacggc cgccggcttc gccgcatctt
     7801 cgatttcggc gaacagctcc tgcttgagcg ccagatcctc cggcgcggct tcaatgacaa
     7861 aatccacatc cgccacggcg gtggcgagtt gagcgtgcaa cgtaacccgg gcggcggcca
     7921 cgggatcgag agcgatggcc ttgaggttgt cgcggattcg cggcagcgcc gtcgctcgcg
     7981 cttccgccat ggcgtcgaac agcgcgacgc gatgacctgc ggcggcgaaa accagcgcga
     8041 taccggcgcc catgagaccc gcgccaatca ctgccatcac gggtttctcg gtcattccct
     8101 caacccgcgg tgtagccgcc atccgcatag agcgtatggc cggtatagaa gtccgaagcg
     8161 cgcgaagcga ggaagagcag cgggccggcg aggtcttccg gctcgccgag ccggcccttg
     8221 gggacgcgcg cgaggaagcc cgcgcgcaca gccttggctt tttcatcgtc cgcaaacatc
     8281 caggcggtga ggggcgagcg aaacaccgtc ggcgcaatcg cattgacggt gatgcctgtc
     8341 gggcccaatt cacagcccag agcgcgggta atcccgtcga tcgcggcctt cgaggcacaa
     8401 taggcggtat agcccgcggg gtggccgagc aacccgcgtg cggacgacat caacacgatc
     8461 ttgccgcctt caccctgggc cagcatttgc ttgctgacgc tgcgcgccat caaccaggac
     8521 tgcgtcacgt tggcgtccat gacgtcgaga aagcgctcgg gagccatctc gacgattttc
     8581 gatacgtcgt tcttgccgga cgccacgacc aggatatcga ccctgccaaa ccggcgcttc
     8641 gcggcctcca cgatactttc gcattcggct tcggaggagg gccgcttgtt gatcggctcg
     8701 acgtcggcgc cgagtttccg acaactgacg gcgatgtctt caagctccct ggctttgccg
     8761 gcggccagca ccagcttggc gcccgccgcc gccaaaacct cggccgcaag tgcgccgaag
     8821 gcgccggacg caccggtgat gatggcaact ttgcctttca cgtcgaacag ggaaagcgga
     8881 ttggcaaggc ccagtgtcat ggtttcactc ccggcggata ggggattacg accagcatct
     8941 tgcaaacgga gttggtgcga ttgatgatct cgcgcacttc ccccggcgcg atggtgcagg
     9001 aatccatcgg gcccagaacg gtctcggtgc cgccgatgat gacggtcatg tggccttcga
     9061 gcacgacata gaccttctcg aacggcgtgg aatcgggtcc agccccgccg ccgggcagaa
     9121 acagcgagta accgatccat tggttggtcg gaccgccggg ctcgaagccc tgcaagcgga
     9181 gcccgacgac accccgatgg ttgggggcgt catacggctt ggcgtcctcg tagcgtttga
     9241 cgtgcatcgc gtttccctct gcctgggcga cctgtttctc ggccttgccc gtgtcgatca
     9301 gccgctggcg ttaatatgcc gccccattcg cgattcggta gttctggctc ttgtcgatca
     9361 tcgccaccag gcgaatgatg cagccatcgc cgcgatccca attccaaggg aagaaggcga
     9421 aggtgacgcg cttgccggtg accgcatcga gatcgccgcc gacgttctca atgccgagaa
     9481 tgccgttggt gaagagcttg cgatggacgg gctcccattc ggggtgggct tgcttccagt
     9541 cgatcccgcc cgaccatttc ttgaattctt cggcaagatg cggatgcagc ggaccgttgc
     9601 gatgcggacc gatggcggtc gccaggggat gatcgttggc ctgcgtgtcg tggccgacga
     9661 ccttgatctt tttctcgacg aaccaatcgc cggccgatgg cacgaagccg gggcatttgc
     9721 agaaatattc ctcgctgtcg cggtatttgt ggtgccagcc ggtattgacg atgatgacgt
     9781 cgccggggcg caccgccttt ccggccgcct tatcgagatc gtcgtacgtg atcggttccc
     9841 acatctcctt cgggatcgag accacaaggc ctgagccgaa gaaatgcggc aacggcactt
     9901 cgtcgatgaa tggcgtgtcc tcgacgacat gggcgggggc gtcgatgtgc gtggtgttgt
     9961 gcatgctggt ggtgatgcgc tgcgacagca ccccggattt ggccatgtaa tggatgcgtt
    10021 cgatcttgac atcttcgaag tagggccagt tcggcgactg gaatccaaag cggtgggaga
    10081 gattgtaaaa ttccaatccc atggggttgt cggtgtttgc ctggaattca atgccgcgga
    10141 ttttgaccac ttgagcttct cccgatgatc tcggcgtcgt tgcccgacgg tgtaccgtat
    10201 tgtggtacaa ctgtattgta tatcggttgt atttgcaagg gagaggcgtg cgcaattatt
    10261 gcgccgcaaa aggcgcagtc gcgcgagctg ccgtaagcgt cagagatttc gggaaataat
    10321 cagcttctcc agcaccgctt ttgtttcaag cagccgccgg gcaatcgtct tgtattcgtc
    10381 cttgaagcgc tggctcggaa ccggaaccga gatcgcgaca tagttgcccg cgggatcgcg
    10441 caacgcgacg ccggtggcgc agacacctga ggaatgctcc tcgaggtcga cggcaacgcc
    10501 gctctgccgc gtaaccctga gatcggcgac gagttcggca aatgtgacct tggtgtgctt
    10561 ggtgcgacgg gcgtattttc ggccgatcaa atcgacaatg gcgtcgtcgt cgatccggcc
    10621 gagatacgcc ttgccgtttg ccgtgcaatg cagcggatag gcatcgccga cgacggacac
    10681 tgttcgaagg cgatgggggc cggtgacctg atcgatgaag acggcctgat ccttcttgat
    10741 gatggataaa tccacggttt ccccgagctc cttcgacagg gccaccagaa acggccgcgc
    10801 cagcgacgca aagtcggtgc ggacggacgc cgcaagccgg agaatggtcg gtccaagacg
    10861 catctttcca ttcggcgagg cggcgatgac gagtttttca acctcgagcg ccgcgataat
    10921 gcgctgcacc gtcgagcggg caaggtcgac gcgctgcgca atctgaccga gactgagacc
    10981 ggcgttctcg tgttccagcg cccgcaggat tgcggcggcc cgcgcgatca cctggacctg
    11041 gcttttctcg ttggtctcga tcgtcttcat gctggctccg gactatttaa acgtggcccg
    11101 cgggcatccc cttgcgggca tgctcacata ttgatacagt gtgtaccata atacggtaca
    11161 acagatcata tggaggaagc cggtgtcaag aatcccttcg attcgcggtg gccacgccac
    11221 ggcacctttt ttatatcgca cattgatgcc gccaaggcat ttacctcgtc ggcgctgatc
    11281 tactcgcaga ttttgtgacg gccgccaccg atcggcggct cgggccgaat gaggagacat
    11341 ccgttggttg gcaagatcgc gctggaggag cattttcttt gtccggggtt cgaggcgtat
    11401 tgggcggcga cagtcagcgg cgtcgcgcct gacgcactgc agcgcatcac ggcacggctg
    11461 tccgatttcg gtgacggccg gatcgaggcg atggataagg ccggaatcgc aaaggcggtt
    11521 ctttcgctgg ctggtccggg cgtgcaggcc gaacccgacc gcgcgacggc ggtcaggatg
    11581 gccgccggcg ccaacgacta tctggccagg gagctccaga agggcaggga tcgttatgcc
    11641 gggttcgggc atcttgcgat gcaggacgca aattccgccg ccgatgagct cgagcgttgc
    11701 atgcgcgatc tcggcttttc cggcgcgatg atcaacggtc ataccaatgg ccaatatctc
    11761 gaccacccct cgcttgcgcc gttctgggag agggccgcag cacttcgcgc gccgatttac
    11821 attcacccga cggacccggt gacaccaagt ccggtcctgg atggttatca ggcgttgcgg
    11881 cgagcgacgt gggagtgggg gttcgagacc ggttcgcacg cgttgcgcct tgtcttcggc
    11941 ggagtctttg cgcgttttcc cgacgcgaag gtcattctgg gtcacctcgg tgaaacgctg
    12001 ccgtatctgt tatggcgttt cgacagccgg gcgaggcttt acggcgtcga attggcccgg
    12061 ccgccgtcga gctacatcaa gtccaatatc ttcgtgacga catcgggcat gttttccgcc
    12121 gaacccttgg cctgcgcgat agccgcgctc ggccacgatc gcgtgatgtt ctcggccgac
    12181 tatccgttcg aagatcctga ggaggccgga tccttcatgg accgcgtcgc cttgccggaa
    12241 gacgttcgcg ccgacatcgc atccaacaac gcaaggcgaa ttctgaaact ttaaagcagc
    12301 aaccgtccgc agccggcgcc aagaccggaa ggaccaacac catcggagga aagcgtgacg
    12361 atctccatat cccgcagggc ttttgcgctc accggagcct gcgccgttat cacctcgcgg
    12421 tcggcgtccg ctcagaccgc cgagctgaag gtgtctcact tcctgccgcc caaccacacc
    12481 atccacaagg cgctggtgat gtggagcgac gaccttgaaa aacagtcggc cggccggctc
    12541 aagctgcgga tctatcccgc cggccaactc ggcggcggac ccaaccggca attcgacgcg
    12601 gcgcgaaacg gcgtcgtcga catggctttc agcctgcatg gtgccacacc gggccgatat
    12661 ccgatgaccg agctcgccag cctgccgttc gcagcaccat cggcgggcaa taaaagtatg
    12721 gtgacatcgc gccggctgag tgagcttgcg ccggagtacc tcgccaagga gcatgaaggc
    12781 ctgcgaattc tgtggatggc cgtgactccg ccgctgatgt tccacagcaa gacgccgatc
    12841 cgcacgatcg ccgacttcaa gggtaaacga atccgctacg ccggcgtgca gttcaagaac
    12901 atcatcgata gcctcggcgc ggttccgctg ctggtccctc cgcaggaaac ccaggacggg
    12961 cttgccaagg gcatcatcga cgcggcgact tttccatacg aaggcgcggc gagcttcgat
    13021 atcgcgaccg ttgccaagta tacgctggag cccggtgtca gttcagcgac cttcgccctc
    13081 gtcatgaacc cggtgaaata cgcttcgttt ccggatgatc tgaaggcctt gatcgataag
    13141 acgaccggcc cggaagccgc gacaggatat ggcgcgatgt gggacgaggc cgaaaacgag
    13201 ggcaaggcgt cgatgctgag caaaggcgcg caggtcacgg tgcttgccga cgaccagctg
    13261 gaacagatga agaccatcct ccgcccgcag cgcgatgccg cggtcgccga cgtcgagagc
    13321 cagggcaagc ccggccggaa gttccttgac gtctatctga aatagctcga cccgagctga
    13381 accaatccgg ttcgtccgcg aggctgccac gaaagcgacg ccgaccagga aaccgacccc
    13441 ccatgaccag ccaggcttcc tcgccctcca cgccgcctcc ggcagtcaag cggttgcaga
    13501 ccgcgcagct gcggctggcc gcggtcgccc tcatgaccat gatgttggga acggcgctgg
    13561 atgttttctt ccggtacgct ttcaacaagc cgttgcggat ttcctacgat ctcgttgaga
    13621 gcatgctggt gatctacgtc ttccatggca tctcgagcgt tttcctggca cggcgaaaca
    13681 tcgttatcga tgtgatcgat tccctggtcg gcccacggat cctgcgcggg cttgcagtga
    13741 tcgctgatgt cgtctcggtg ttgtgcctcc tgctcttcct gtggggcatg tacgaacctg
    13801 cgctgcaggc ctaccagtat ggagatcgca agctcgagct tggcgagccg ctctatgtgc
    13861 tatgggcatt cgcgctcgcc ggactgctcg gcaccctcgt ttgcgccatt gccgccttta
    13921 cccacatcac attgcgccag tttcggagac actcgtgagt cccgcgatta tcggcgcgct
    13981 aggcatttcg tcactccttg tattgctgtt cgtgcgggta ccggtctggg ccgcgctgac
    14041 actggtcggc ttcaccggca atttcacgct gtcgggtctg catagcgcat tgagcctggc
    14101 cggcacttcc gcattcgata tgtcctccgc ttacaccctc tcggtcatcc cgctctttgt
    14161 cctgctcggc gaagtggcgt caaacacgcg gctttcggct gatctgttca acgcggcgcg
    14221 tctgcttcta tccggcgtcc gcggcgggct ggcgatcgca acgatcgctg cgtcctcctg
    14281 tttcggtgcg gtatgcggat cgtccattgt gacagccgcg accatgacgc ggatcgcgct
    14341 gcccgagatg cgcaaggccg gatatgatga ccggctggcg accggggcgc tcgccgcggg
    14401 cggcagtatc ggcattctga ttccgccgtc gatcatcctg gtgatctacg cctcgatcgc
    14461 cgaacagtcg gtgccaaaac tgtttgccgc agcgctgttg ccgggcctgt tactgacggc
    14521 gctcaatatg acggtggcga tcggcatcgc catagtgtat ccgcactacg cgccgcgcat
    14581 ggagaaggcg tcggcgcgcg agcgattcaa ggccctggct ggtccctggc aattcatcct
    14641 cctgttcgtc atcacgattg gcggtatcta tgccggcatc tttagcccga ccgaagcggc
    14701 ggcgatcggc gcgttcggca gcatcgtgct cggctttctc ggtcgccgcc tgacctgggc
    14761 gacgctgtgg cgttcgatcg agacctcgat caccaccagc tgcctgctat tcgtgatcat
    14821 catcggcgcc aacctgtttt cttcgttcat ggtgcaaacc cagtttccga cgatgctcgc
    14881 ggacggcgca agcgccatga acctttcggc gcccgtcgtg atgatcatga tcgtgctcgc
    14941 ctatatcgtg atggggtgct tcctggaggg tatcggcatg gtgttgatca cggtgccggt
    15001 gttcctgccg ctggtcgtca agttcggcta tgatccaatc tggttctcga tccttgtggt
    15061 gatcgttgtc gaagtcggcc ttattcaccc tccggtcggc atgaatctgt tcgtgattca
    15121 ggcgcaggcg ccggatgtga agatcgcgag catctaccgc ggcatcgtcc cgttcctgat
    15181 cgcgccgctt gtcctcatca ttttcctgtt cctgttcccg agcctcgctt tgtggcttcc
    15241 gaagctgctg tatggctgat cgctcatgac caatgtcctg gtactcgaaa cccatgcgga
    15301 ggcctatgcc gccgccttgc gcgaagaatt tcccgacctg gagcttcagg tcgcgcgaac
    15361 gccggccgat gcccacgtcg actacggcaa ggtcgagata ttgattgcat tcggcatgga
    15421 cgtcacggat ttgctcttta cgggaatgcc ggggctcaaa tgggtgcaat cgctggcgac
    15481 cggcgtcgat cattttctgc gcagcaagac gttggcccgc gacatcatat taaccagcgg
    15541 ccgtggcatc cacggacctg cgatgcgcga aaccgttctg cacttgatgc tttcgatcag
    15601 ccatgacagc aacggtttgg tccgcgataa ggtcggtcgc cgctgggatc gccgcgcatg
    15661 gcccttgctt gccggcaaaa ccgcggccgt gatcggcacc ggggtcgccg gttcagcgat
    15721 cggctcgttg ctgcaggcat tggagatgcg ggtggtcggg gtcagccgga caccgcggca
    15781 ggtcgccgga ttcgacacgg ttgtaccgca aagcgaattg tcggaggctg ccgcgaaggc
    15841 ggactacgtc atcaacgtcc tgccggccaa cgcagtgaat ctcaagatca tcggcagcga
    15901 tgtctttgtc cgcatgaaac caggcgccta tttcatcgat gtcgggcgtg gagaaacggt
    15961 cgacgaggaa gcgctgattg aggtcctgag ggatggcaag atcgccggcg cggggcttga
    16021 tgttttcgcc accgaaccat tgccggcgca gagcccgtta tgggatcttc ccaacgtctt
    16081 catgagcccg cacatcggcg ggctgttcga ggaatatcag cagatggcga tgcccctgat
    16141 catccgcaac atgagcgcct atctctccgg ccgcaccgac cagatgacga acgtcatcga
    16201 tcgttcgagg agcgtcacat gaccggcatg atcgatcccg ccagccgcat cctcacccgg
    16261 atctccgaga ccgaattgaa ccggcgctgg aatgcggttc gtcaggcgat gaagcagcgc
    16321 ggcatcgacg cagtcctgat gcagaacacc aacgactggc tgggcggcta tgtgaagtgg
    16381 ttcaccgacc ttccggccaa caacggctac ccgcgcaccg ttgtttttca cgcggatgcc
    16441 ccgatgagcg tggtcgagat ggggccgtcg aatatccggc ggacgttcgc ttccgacgaa
    16501 acgctgcacc ggggcgtcgg cgagatgctg catacgcctt cgttcacatc gatcgggtat
    16561 acggatcgcg atgacgtgca cctggcgatc gatgcgcttc gtcgccactc caatcgcacg
    16621 ctcggcctga tcacgccggg agcgctgcct tacgcgtttg tctccgcgct gaaggcggcg
    16681 tttgccgcga cgacattcat cgatgtcacc tccgacatcg acgccatcaa ggcgatcaag
    16741 agcgaagagg aagtcgccct cattcgacaa accgcgcgtc tccaggacga ggcgttcgcg
    16801 gaggttctca aggaaattcg ccccggcctg cgcgacatcg acgtcaccgc gatcgcgcag
    16861 cggaaagggc agctgctcgg cagcgagcaa ggcatcttcc tcggcggttc agcccccgtc
    16921 gggataagat cgcctttcgt cccgcgttac atgcagggca ggcggctgga gaagggcgat
    16981 catctgtcgc tcctgatcga aatcaacggc cccggcggct tctacacgga aattgcgcgg
    17041 accatcgtgc tcggcaaagc ttccgccgaa ctgaaagagg cctttgcgtc cgtcaaggcc
    17101 gcgcaggacc actccctcag catcatgaaa ccaggaacgc aggtgaggga cgtcttcgca
    17161 agtcacaatg cctatatggc gagcaacggc atgcccgccg aaacccggct ctatgcgcac
    17221 gggcaaggct acgacatggt ggagcgcccg ctcatccgcc aggacgatga catgacgcta
    17281 cagcaaggca tgtgccttgc tgttcatccc ggatatgaga cgccatcgct atttgccgtg
    17341 atttgcgaca actacgtgat tgaggctgac ggccccggtg ggtgtctgca ccatacggag
    17401 aaaaggttgt ttgaaatcga ttgaccatgg gaggcgaggc gtggaacgtc tcgcaagcca
    17461 acgcgccgag ccgcggctag ttcttagtac gaataaaccc ggcaagcctc gtcttctgag
    17521 tctcgcacca acttgtcatg ccgaggcgtg tctggtccag gtccgttgac tttgtcgcca
    17581 aggcgacttc agccattaga tcgtcatcct ggcgctcgcc cattttccca cccgtgtgca
    17641 tgtaagcgat ggccttccgc accttctcgg tggttccgtc gggcagatgc atctcctggg
    17701 ccttctgcac cagatcctga tagacgatgt ccatgccggg gcattccacc ttggcggcga
    17761 aagcctgcaa gaccattgtc acatacgccg agcgagactc ggcatgcgcc gagctcgcaa
    17821 ggacaagcaa ccccaggatg aatggaccat agcgcatcgc cggctccctc gttcggggca
    17881 aactagcgct gacggcttca ctgcgcaacg tccggaagcg agggagcgcg gagcctgcag
    17941 ttccaacgtt tgatcagctg cgggaacctg caaagggccc gatcgatcca ctggtcttgg
    18001 tcaacaggac gaacaagcat ggcgggtcct ccgccaaccc cgcccgccgc gtcgcttcat
    18061 ggcgagcata ctattatggc gacgcatgta ccttcgtctt gcccaacggc gatttcaggc
    18121 ggccgcttct ggcgcaaagc ggccgtcgac caaacatccg cttcttcgag tctatgctcc
    18181 cgctcactgg tgtcgcggct gctgacagcc gatcgcggtc tgggccgcct tgcaggcgct
    18241 gctcgccggt gcgacacttg ccgtcaagtc cgacggcaaa gaccgggcaa aacgggcatg
    18301 cgggacgata gagggaacga tgaagctcgc tgcgaacctg ttcgccgccc tgcttgtgct
    18361 gctcgcggcg cagacggcat cggcacaatc cagttatccg aaccggccgg tgaaaatcct
    18421 ggtcggcttc acacccggca cggctcccga cctttcggcg cgcattcttg cggaccagtt
    18481 ttcggaagtc tggggcacgc cgttcgtggt cgagaatatt cccggcgccg gcagcaacat
    18541 cgccaccgaa cgcgtcgcca aggcggccgc cgatggctac acattgctga tgggaggcaa
    18601 ctcggcgctg gtcatcaatc cgagcttgta tgaaacgctg ccattcgatc cgctcaagga
    18661 cttcgaaccg atctcgcagg tattcatcgc ggcgaacgtg ctcgccgttc cgcccgagct
    18721 gccggtcaag agcgtggccg aactggtggc gctcgccaaa gcggagcccg gcaagctttc
    18781 ttatggccat gcaggcgtgg gcacgtcgca gcatctgggc gccgaattgt tcaaattcat
    18841 ggcgcatgtc gatattgcac cagtgcccta tcgcggcacc acggcgttaa tgccggatct
    18901 gctcgccaac cgaatcaaca tgtcgttcgc caatatcgtg aacgtggtcc cgctcgcgcg
    18961 cgaagggaaa ttgcgcgcgc tggccatcac ctcgatcaag cgctcggccc tggcgcccga
    19021 cctgccgacc atggcggaat ccggatttcc cggcttcgag gcggtgccgt ggttcggcct
    19081 gctcgcgccg atcggcacgc cgaaggatgt tctggataaa ttgcacggcg aaaccgtcaa
    19141 ggccctggcg atgccggagg tgcgcaagaa gttcgacgaa cttggcctcg agccggtcgg
    19201 cgacacggcg gctgagtttg ccgcgatcat caagaaagaa acgcccgagt gggcaaaagt
    19261 catcaaggat gccggcatca agctcggcaa ctagctcggc cttccaggcc caacactttg
    19321 gaagatcgtc gacgcgtcgc aatttgccgg gctgcccggc cgagagcaaa gcgatggtgg
    19381 gggcaatcct ggacgcttat ctacaccggg taacctcttg cctcaaatca gggtcgtccc
    19441 gtgcgtggcc gcagccatcc catgatcacg tcgtgcggcg ttcggcggct gcgtggcagg
    19501 ctgtgtcgct ggaccgcggc ttttgcaaac gctagtgcct gctggccgcg agcgctgccc
    19561 cgtggataag cgccgcgccc ggcggccacc ttgggactgc aattccacgt cgcgacgtgg
    19621 catgtctgct tgttcggccg ttcccctcag tcgtgctcgt agccgtagac ctccggcagg
    19681 ataaagaccg ccgccagcaa cccgatcagc ggaaagattg caacgaaaag cgtggcgttg
    19741 gcttggccga tcgccgcgaa cagcgacggg aacaggaaga tcgcgaggaa ggacggcagt
    19801 ttgacgaaca tgtaggcgaa gccgctcgcc gtgccg
//
LOCUS       DATNOI010000118         2147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_498394,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000118 DATNOI010000000
VERSION     DATNOI010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498394"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="VN968_04380"
     CDS             <1..161
                     /locus_tag="VN968_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HXL28511.1"
                     /translation="LKFFAWAYAKGGKMAEELDFVPMPTSVVTAVEGVWTKEIKDASG
                     KPLFTAAH"
     gene            421..1323
                     /gene="pstC"
                     /locus_tag="VN968_04385"
     CDS             421..1323
                     /gene="pstC"
                     /locus_tag="VN968_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HXL28512.1"
                     /translation="MVFRHLTRAAAILVLIILSGVIIALIKGSLPALQAFGLDFLIEE
                     RWNPVTEKFGALAPIYGTLVTSFIAMLIAVPLGLLIALFLTELCPMWLRRPIGIAIEL
                     LAGIPSIIYGIWGLFVFAPFLQRTLQPALIDSFDNVPFLGALFAGPPYGIGILTASLI
                     LAIMVLPFITSISRDVFEAVPPVLREAAYGVGCTTWEVVRHVVLPFARVGVIGGVMLG
                     LGRALGETMAVTFVIGNAHKVSASILAPGTTISATIANEFTEAVGDLYTSSLIALGLI
                     LFVITFIVLAAARFLLLRLEKRVG"
     gene            1326..>2147
                     /gene="pstA"
                     /locus_tag="VN968_04390"
     CDS             1326..>2147
                     /gene="pstA"
                     /locus_tag="VN968_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HXL28513.1"
                     /translation="MDRYGWRRLGNTIVITLSIGATVLGLGWLVLILGSLVYQGLSGL
                     SLAVFTQMTPPPGADGGLLNPIMGSLMLTVLAIAIGTPIGILAGTYMAEYGKAAVLSV
                     VVRFINDILLSAPSIVVGLFVYEIMVAPMGHFSGWAGAVALALIVIPVVVRTTEDMLS
                     LVPRELRESATALGLPRAHVIRYVSYRAARAGIITGVLLAIARISGETAPLLFTALNN
                     QFFSTNLNAPIASLPVVIFQFALSPYADWQKLAWIGALIITFAVLALSITARALSA"
BASE COUNT          358 a          652 c          646 g          491 t
ORIGIN      
        1 cactcaagtt ctttgcctgg gcgtatgcca agggcggcaa gatggctgaa gaactcgatt
       61 tcgtgccaat gccgacctca gtcgtgaccg ccgttgaggg cgtctggacc aaggagatca
      121 aggacgcaag cggtaaaccg ctcttcacgg cagctcatta aattgttgcg atcaacgcgg
      181 aagggcgccg gtggcgcctt tccgtgcctc gcgtatcatg aagccgcggc atcgccttgc
      241 aaaggtgccg ggatgagaag tgagtggctc tccctacgat ggccgaactg agggacgcag
      301 ccgaaagtgt ttgaggcagg gagaaagaaa ttggccgaca tagcgctatc cgggaacgcc
      361 gaagcgtcgg tggcggcaac gtctcgcact aaaattctcc gccggttgcg atttagcgaa
      421 atggtgtttc gccatctgac ccgcgccgcc gccatactgg tgctgataat tctgagcggg
      481 gttatcattg ccctgatcaa gggctcactg ccggcgctgc aggctttcgg actcgatttc
      541 ctgatcgagg agcggtggaa tccggtcacc gaaaaatttg gcgccctcgc gccgatttac
      601 ggcacgctgg tgacgtcgtt cattgcgatg ctgatcgcgg tcccgctcgg gttgctgatc
      661 gcgttattcc tcaccgaatt gtgcccgatg tggctgcgtc gaccgatcgg cattgcgatc
      721 gagctgctcg ctggaatccc cagcattatc tacggcatct ggggcttgtt cgtgtttgcc
      781 ccgttcctgc agcgcacgct gcaaccagcc ttgatcgact ccttcgacaa cgtgccgttc
      841 cttggggcgt tgttcgcagg gccgccgtac gggataggca ttcttaccgc cagtctcatt
      901 ctcgccatca tggtgctgcc gttcattacg tcgatttcgc gcgatgtctt cgaggcggtg
      961 ccgcccgttt tgagggaagc cgcctacggc gtcggctgca caacctggga agtggtgcgt
     1021 catgtggtgc ttccctttgc gcgcgttggc gtcatcggcg gcgtcatgct cggtctcggc
     1081 cgcgcgctcg gcgagaccat ggcggtgact ttcgtcatcg gcaatgccca caaagtttcg
     1141 gcatcgatac tggcgcccgg caccacgatc tccgccacca tcgccaacga attcaccgaa
     1201 gccgtaggcg acctctatac gtcgtcgttg attgcactgg ggctgatcct gttcgtgatt
     1261 accttcattg tgttggcagc cgctcgtttc ttgctgttgc ggctcgaaaa gcgggttggg
     1321 tgaacatgga ccgttacggc tggcgccgtc tgggcaatac gatcgtcatc acattgtcga
     1381 tcggtgccac agtgttggga ctggggtggc tcgtattgat cctgggctcg ttggtttacc
     1441 aaggactgag cggcctgtcg ctggcggtat tcacgcaaat gaccccgccg cccggagccg
     1501 acggcggctt gctcaacccc atcatgggca gtttgatgct gactgttctg gcgatcgcga
     1561 tcggcacgcc aatcggcatc ctggccggaa cctatatggc ggaatacggg aaagccgccg
     1621 tgctcagtgt cgtggtgcgc ttcatcaacg acatccttct gagcgcccct tcgatcgtcg
     1681 tcggcctttt cgtctatgaa atcatggtcg cccccatggg acatttttcc ggatgggccg
     1741 gcgccgtcgc gctcgcgctg atcgtgatac cggttgtggt gcggacgacc gaggacatgc
     1801 tttctctcgt tccgagggaa ttgcgcgagt ctgcgaccgc gctcggattg ccgcgcgccc
     1861 acgtgatccg ctacgtctcc taccgggcgg cccgcgccgg catcatcacg ggcgtattgc
     1921 tcgccatcgc ccgcatcagc ggcgaaacgg cgccgctgct gttcaccgcg ctcaacaatc
     1981 agttcttcag tacaaatctc aacgcgccga tcgccagtct tccggtcgtg atcttccagt
     2041 tcgcgctgag cccctatgcg gactggcaaa agctcgcctg gatcggcgcc ttgatcatca
     2101 ctttcgccgt gctcgccttg agcatcactg cgcgcgcgct ctcagcg
//
LOCUS       DATNOI010000119        12241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1883441,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000119 DATNOI010000000
VERSION     DATNOI010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12241
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883441"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VN968_04395"
     CDS             complement(<1..951)
                     /locus_tag="VN968_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HXL28514.1"
                     /translation="MNANLRNFALWVIIVLLLLALFTLFQNPGQRASSQDISFSQLLT
                     EVDQNHVRDVVIQGPEIHGTFTNGSSFQTYAPNDPTLVSRLYNGKVSITAKPPGDNVP
                     WFVSLLVSWLPFIALIGVWIFLSRQMQGGAGKAMGFGKSRAKMLTEAHGRVTFEDVAG
                     VDEAKQDLQEIVEFLRDPGKYQRLGGRIPRGVLLVGPPGTGKTLIARAVAGEANVPFF
                     TISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDERE
                     QTLNQLLVEMDGFEANEGVILIAATNRPDVLDPALLRPGRF"
     gene            complement(1208..2341)
                     /gene="tilS"
                     /locus_tag="VN968_04400"
     CDS             complement(1208..2341)
                     /gene="tilS"
                     /locus_tag="VN968_04400"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXL28515.1"
                     /translation="MLDDDHSPISAQHAKRLFADWKAAPAIVLAVSGGPDSIALMWLA
                     ARWRRTLARGPRLIAVTVDHGLRAEAKREARDVKRLAQALDLPHRTMRWTGAKPKTGL
                     PAAARAARYRLLARAARASGATHILTAHTRDDQAETLLMRLLRGSGIAGLGAMARQSE
                     RDGVLLARPFLHVSKSQLIATLKKAKLGFADDPTNRDPSFTRPRLRTLMPVLAAEGGD
                     TRNLARLASRLARANQAVEVLVDGAERYLALRDREASRPEPGAGAKAFKAKTSDAKTF
                     DAKTFDAKIFDAEAFVAMPEEIRLRLLKRAIDRFGHEGPAELGKVEALLEVVDRAVAE
                     SRRGLLPSPSQSRLKQTLAGALVSLTDGRIRVEPAPPRRSRAD"
     gene            complement(2391..3425)
                     /gene="ybgF"
                     /locus_tag="VN968_04405"
     CDS             complement(2391..3425)
                     /gene="ybgF"
                     /locus_tag="VN968_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HXL28516.1"
                     /translation="MSSRFHQIACAAAMSAMLALSSQVSAQSDDVDPEMRIQQLENQL
                     RQLTGQNEELQYRNRQLEDRLRSLQGGAPGAPVGQPAVAQPNIAAAAPPVQPYPPPGQ
                     PQAQQGYNQQGYGQQIAPPAPIAAESPGVPPAPQGRRRGDAFDPNQNPNAPGAPRALG
                     GGQRPVPAEAPIGASGGRGAGEPLDLANTAPRNPAGALPPPPRNANAAGTLTTLPPSA
                     TPKDEFDLGIGYMQRKDYALAEETMRNFAQKYPSDPLTADSQYWLGESFFQRQLYREA
                     AESFLAVTTKFDKSAKAPDSLLRLGQSLAALKEREAACAAFGEVTRKYPRASSGVKAA
                     VDREQKRVKC"
     gene            3732..4268
                     /locus_tag="VN968_04410"
     CDS             3732..4268
                     /locus_tag="VN968_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL28517.1"
                     /translation="MAVPGFDHSIYRPAYSALADVAQIVYLDHRGNGRGEDGPHAGWN
                     LAQWGDDVHAFCDVVGVESPIVLGASFGGMIALAYATRHPAHPSKLVLISTEAAGATH
                     RDRRVALFERFGGAEVGALALLEDDPIHPIESQADIAAALPAHLVQFERFANCRHAVV
                     PDAPERAMAVIRDFIGRK"
     gene            complement(4335..4826)
                     /gene="pal"
                     /locus_tag="VN968_04415"
     CDS             complement(4335..4826)
                     /gene="pal"
                     /locus_tag="VN968_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HXL28518.1"
                     /translation="MKHQMRILQGLKLAAVLAVALSMGACANKNLGADGAMASAATPG
                     SQQDFVVNVGDRVFFESDQTDLSPQAIATLEKQAQWLQSYNRYSFTIEGHADERGTRE
                     YNIALGARRAQSVRTYLASRGIDPSRMRTISYGKERPVAVCNDISCWSQNRRAVTVLN
                     ASS"
     gene            complement(5169..5903)
                     /locus_tag="VN968_04420"
     CDS             complement(5169..5903)
                     /locus_tag="VN968_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28519.1"
                     /translation="MRPAAEAIALVPERTADPLEHVDYRLAETPEDKDRIYRLRYRAY
                     LREGAILPSESERVSDRYDDLPNNFTFGVYVKGELYSSIRISVLTSEHRGSPSSEMFT
                     ELLHPELDRGKVIVDPTRFVADPEKARTFPELPYVTVRLGYIACGHFNADIGLANVRP
                     EHRAFYRKVFLQEPWGEPKLYPGLIKPVGLVAAHYPSIRERVFQRFPYMRSSAFERRM
                     LFQRRGERDSSSPGAMAQPTASLPRS"
     gene            complement(6085..8418)
                     /locus_tag="VN968_04425"
     CDS             complement(6085..8418)
                     /locus_tag="VN968_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXL28520.1"
                     /translation="MQLASETGDPDEKMPPSIYAALIDSLFQDPGPLFAGALCAAAGA
                     IMTAVKTGNPWLWPSVALLVVTGAVRALDMRQYRQRNCGLTSNEAARWEVRYQFGAML
                     YAAALGLWCMVTVLGSSDAVAHMIAISVTLCYVAAGGCRTYGRPWIFHVQMLLACGPL
                     TLALALYGSPYYFGMAVLNVLFFLALKHISTSLQKIFVRALVAREREAALASQFDTAL
                     NNMPHGLCMFRGDGRLAVVNHRFTAMMNLRDDLVHRGAGAPDIVDACVSAGSLSAASG
                     RMILSEIGNSQAKDIITTDSSPASGRSLAWTFQSMAGGGTVVLVEDITERKNAEARIS
                     HLARYDELTALPNRVNFRDEIERLLAISHSAERLSALLFVDLDQFKQVNDTLGHPCGD
                     QLLCAVADRLREMLRPEDFVARFGGDEFVVFQQNILSNEDAAGLARRIVDRLSERYKI
                     DNHLVEIGASVGIAMTSPAGISADTLLKNADMALYRAKADGRGTFCFFRDEMAQTVEA
                     RRILELDLRKALAHEEFELFYQPLVNLKSGRIATCEALLRWNHPVRGTVSPIDIIPVA
                     EDMGLIVDLGRWILRKACMECMKWPEGVSVAVNFSPQQFHQRDVLSEVRYALEVSGLP
                     AHRLEIEITESSLLRNTQLTHDVLSQLHMIGVRISLDDFGTGYSSLSYLHNFPLQKVK
                     IDRSFLEGIDSDRPLTLLRGVARLSADLGMSVVVEGIETNEQLELISVDGTVSEAQGY
                     LFSRPVPAVRIRQLLNASHGRRLPDEKLLELPSRSIA"
     gene            complement(8602..9882)
                     /gene="tolB"
                     /locus_tag="VN968_04430"
     CDS             complement(8602..9882)
                     /gene="tolB"
                     /locus_tag="VN968_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HXL28521.1"
                     /translation="MMRPGGLAAQGQQRLEVVPGGTQAIPIAIPNFAAGTPSDGEAGV
                     NVAQVITNNLRRSGLFAPIDQAAYIEKTINIDAAPQFQNWKTINAQALVTGRMTRQPD
                     GRLKAEFRLWDVTTGQQITGQQYFTSPEYWRRIAHIISDQIYEHLIGDKGYFDSRVVF
                     VDESGPKERRVKKLALMDQDGANVRYLTKGADLVLTPRFSPARDEQLITYMEFGQGDP
                     RVYLYRIETGEREIVGNFPGMSFSPRFSPDGQRVIMSLQQGGNSNLFVMDLRSKSTTR
                     LTDTPAIDTSPSYAPDGTRLCFESDRGGKPQIYVMPATGGPAQRISFGEGSYSTPVWS
                     PRGDYIAFTKQGGGQFSIGIMKTDGSGERILTSGFHNEGPTFAPNGRVLMFFRDPGGS
                     GGPSLFTVDVSGRNEQRVPTPGFASDPAWSPLLS"
     gene            complement(9983..10915)
                     /locus_tag="VN968_04435"
     CDS             complement(9983..10915)
                     /locus_tag="VN968_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolA"
                     /protein_id="HXL28522.1"
                     /translation="MKVDKTLVASVALHVVVLGWGLVSFSSRAFESTEIESVPVEFVT
                     DNELSKITAGIKSGKKENPKPLVEKVAEAKPMDDAVGKITEKKPVVTDAAPDPTPKPV
                     EKPVEKTPDPPKPVAETKPKDEPKPIEKKPDPPKVDSIAEALKKEEAKKPAPKPAAKA
                     APPPPVKKREYVFDRESISALVDKQAPSRQAFAGDALNSTAALGTAKGTAATPSASYL
                     SALVSRIHDCWQRDGGGFDEDDLKIPITLTFKVDGTLSAAPLIEIEPRNIRERAIFEG
                     ARRAIIACQPYTMLPQAKYAEWKVLPFLFHNKAY"
     gene            complement(10918..11367)
                     /gene="tolR"
                     /locus_tag="VN968_04440"
     CDS             complement(10918..11367)
                     /gene="tolR"
                     /locus_tag="VN968_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HXL28523.1"
                     /translation="MAMNMAGSSGGGGRRGRRKAVMAEINVTPMVDVMLVLLIIFMVS
                     APLLTVGVPLDLPQTQAKSLDQDKTPLQLSVDVQGKVFINNTEVAMNELIPKLKAITD
                     ARGGMDERIFMRADKKADYGTVARIMGQLSGAGFKRLALVTEVEQGS"
     gene            complement(11407..12120)
                     /gene="tolQ"
                     /locus_tag="VN968_04445"
     CDS             complement(11407..12120)
                     /gene="tolQ"
                     /locus_tag="VN968_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HXL28524.1"
                     /translation="MNPADVAQSALPLASTDVSLIALFLQAHWIVKAVMLGLLACSVW
                     VWAIAIDKTFLYARTKRAMDRFEQAFWSGQSIEELYRALSAKPTQSMAACFVAAMREW
                     KRSFESQARSFAGLQMRIEKVMNVSIAREIERLERRLLVLATVGSAGPFVGLFGTVWG
                     IMSSFQSIAASKNTSLAVVAPGIAEALFATAIGLIAAIPATIFYNKFTSEVNRQAQRL
                     EGFADEFSAILSRQIDERA"
BASE COUNT         2319 a         3812 c         3795 g         2315 t
ORIGIN      
        1 gaaccggccc gggcgcagca gcgccggatc gagcacgtcc ggccggttgg tcgccgcgat
       61 caggatcacg ccttcattgg cctcaaaacc gtccatctcg accagcaact ggttcagcgt
      121 ctgctcgcgc tcgtcattgc cgccgccgag gccggcgccg cgatggcggc cgaccgcgtc
      181 gatttcgtcg atgaagatga tgcagggcgc gttcttcttg gcctgttcga acatatccct
      241 gacgcgggag gcgccgacgc cgacgaacat ttcgacgaag tcggaacccg agatcgtgaa
      301 gaagggcacg ttggcttcgc ccgcaaccgc acgcgcgatc agcgtcttgc cggtgccggg
      361 agggccgacc agcagcacgc cgcgcggaat ccgtccgccg agccgttgat actttccggg
      421 atcgcgcagg aactcgacga tctcctgcag gtcctgcttg gcttcgtcga caccggcgac
      481 atcctcgaag gtgacgcggc catgcgcctc ggtcagcatc ttggcccgcg acttgccaaa
      541 gcccatcgct ttgccggcgc cgccctgcat ctgccgcgac aggaagatcc acaccccgat
      601 cagggcgatg aagggcagcc aggagacgag gagcgagacg aaccacggca cgttgtcgcc
      661 gggcggcttc gcggtgatcg ataccttgcc gttatacagg cgcgagacca gcgtcgggtc
      721 gttcggcgcg taggtctgga aactggaacc attggtgaag gtaccgtgaa tttccggtcc
      781 ctggatgacg acgtcgcgaa cgtgattctg atcgacttcg gtcaaaagct gcgagaacga
      841 gatgtcctgc gaggatgcac gctgaccagg attctggaaa agcgtgaaca gggccaacaa
      901 cagcaggaca ataatgaccc acagggcgaa attacgcaga ttggcgttca tcgatcttcc
      961 ttcgtggtcg cgcggatcgc ggccttatat ccttggatag tgtccttgag gtaggcacga
     1021 ggaatccccc ggtcgactgc aatacaatct aggtgccacc ctggtcgctg ccaagggaac
     1081 ctcataggac tatttagcgc atattggagc gattcccggt caaataatga tgctgaaaag
     1141 ggtgattccc gaagtggtta aggctccggc atggaacgag aaccacgatg ccagtgttca
     1201 atttccctca atcggcccgg cttcggcgtg gtggcgccgg ctcaacgcga atccggccgt
     1261 cggtcaggct taccagtgca ccagcgaggg tctgtttcag ccttgattgg ctagggcttg
     1321 gcaagagccc tcttctgctt tccgcaacgg cccggtccac cacctccagc aaggcttcga
     1381 ccttgccgag ttcggccggc ccctcatgtc cgaaccgatc gatggcgcgc tttagcagcc
     1441 gaaggcggat ctcctccggc atggcgacaa aggcctcggc atcgaagatc ttggcatcga
     1501 aggtcttggc gtcgaaggtc ttggcgtcgg aggtcttggc cttgaaagcc tttgcgccgg
     1561 ctccgggttc cggacgcgag gcctcgcgat ccctcagcgc gagatagcgc tcggcgccat
     1621 cgaccagaac ttccacggcc tggttggccc gggcaagccg cgacgccagc cgcgccagat
     1681 tgcgcgtatc gccgccttcc gcggcaagaa ccggcatgag cgtgcggaga cgaggccggg
     1741 taaaactggg atcgcggttg gtcggatcgt cagcgaagcc gagctttgcc ttcttgaggg
     1801 tcgcgatgag ctgcgatttc gaaacatgca gaaacggacg tgccaacagc acgccatcgc
     1861 gctcggactg gcgcgccatc gcgccaagac cggcgatgcc gctgccgcgc agcagccgca
     1921 tcaacagcgt ctccgcctga tcgtcacggg tatgcgcggt gaggatatgc gtggcgccgc
     1981 tggcccgcgc cgcccgcgcc agcagacgat atcgcgcggc gcgcgccgcg gccggcaatc
     2041 cagttttcgg cttggcgccg gtccagcgca tcgtgcgatg cggcagatca agggcttgcg
     2101 ccaggcgctt gacatcacgt gcctcacgct tggcctccgc gcgcaagcca tgatcgacgg
     2161 taacggcgat gaggcgcgga ccgcgcgcca aagtgcggcg ccagcgggcc gcgagccaca
     2221 tcaacgcgat cgaatccgga ccgccggaaa ctgcgagaac gatcgcaggc gcggccttcc
     2281 agtccgcgaa cagccgcttt gcatgttgtg ccgagatcgg ggaatggtcg tcgtcgagca
     2341 tggcgttcgg aaacccggtt tccgcctcgg atcagttccg aggccacgct ttagcactta
     2401 acccgctttt gttcacgatc caccgccgct ttgacgccgc tcgaggcgcg aggatatttc
     2461 cgcgtcacct cgccaaacgc ggcacaggcg gcctccctct ccttcagggc cgccagcgat
     2521 tgtccgagcc gcagcaatga gtcgggagcc ttagccgatt tatcgaattt ggtggtcacc
     2581 gcgagaaagg actctgcggc ctcgcgataa agctggcgct ggaaaaagct ctcgcccagc
     2641 cagtattgtg aatcggcggt caagggatcg ctggggtatt tttgcgcgaa gttgcgcatg
     2701 gtctcttcgg ccagcgcata gtccttgcgc tgcatatagc cgatgccgag atcgaactcg
     2761 tccttcggcg ttgccgacgg cggcaaggtt gtcagcgttc ctgccgcgtt ggcgttgcgc
     2821 ggcggtggcg gcaaagcgcc cgccggattg cgcggagccg tgttggccag atcgagcggc
     2881 tcgcccgccc cgcgcccgcc cgaagccccg atcggcgctt cggccggaac cggacgctga
     2941 ccaccgccca gggctcgcgg cgcccccgga gcattcggat tctgattggg gtcgaaggca
     3001 tcgccacggc gccggccttg cggtgccggc ggaacgcccg gtgattccgc ggcgatcggc
     3061 gccggcgggg cgatctgctg gccataacct tgctggttat agccttgctg ggcctgtggc
     3121 tgcccgggcg gcggatacgg ctgcaccgga ggcgccgctg cggcgatgtt gggctgggcg
     3181 acggcaggct gaccgacggg ggcgcccggc gccccgccct gcagtgacct cagccgatct
     3241 tcgagctgcc ggttgcggta ttgcagctcc tcattctggc cggtgagctg ccgcagctga
     3301 ttctcgagct gctgaattcg catttccggg tcgacgtcat cggattgcgc tgacacttgc
     3361 gaggacagcg ccagcatggc ggacatggcc gccgcgcacg caatttgatg aaatctggat
     3421 gacattgggg cctgacgacg tattcgacct gacatcgaaa gcggagcaca ttgagagagg
     3481 gagtacgcca aaaatgtgac tgggtcacat ccggcgttcg tctcatgcag catggtgtaa
     3541 tgttccgtgc gaaacaatca agcggcctga gcagccggat cgatcgccga ttcgcaacga
     3601 ggctggttcg caagggagat ttggccatgc gggtactcgt caacggggtg cgctgttctt
     3661 cgatgttgaa ggaacaaaac tagtcccgga tggtcccgcc atgcgggaac ggccgacatt
     3721 gctgctgctg catggcggtc cccggcttcg accactcgat ctacaggccg gcctactcgg
     3781 ccttggcgga tgttgcgcag atcgtctatc tcgatcatcg cggcaatggt cgcggcgagg
     3841 atggtcctca cgcgggctgg aacctggcgc agtggggcga tgacgtgcat gcgttctgcg
     3901 acgtcgtggg cgtcgagagc ccaatcgtcc tcggcgcgtc atttggcggc atgattgcgc
     3961 tggcttacgc gacgcgccat ccggcccacc cctccaagct cgtgctcatc agcaccgaag
     4021 ccgctggagc cacccatcga gaccggcgcg tggccctgtt cgaacgcttt ggcggcgcgg
     4081 aagtcggcgc ccttgcgctc cttgaagatg atccgataca cccgatcgag agccaggccg
     4141 atatcgcagc ggcgctgccg gcgcatcttg tgcaattcga acggtttgcc aattgccggc
     4201 atgcggtggt ccccgatgcg cccgaacgtg ccatggcggt gatccgggac ttcatcgggc
     4261 gcaaatgagc caagccgcca aaacaaagcc ggcgcccgaa ggcgccggcg aaaggctttt
     4321 ttggagtgaa cgcgttaaga actggcgttc agcaccgtga ctgcgcggcg gttctgcgac
     4381 cagcacgaga tgtcgttaca caccgcgacc gggcgttcct tgccgtaaga aatcgtccgc
     4441 atccggcttg gatcgatgcc acgcgatgcg aggtaggtac gaaccgattg ggcacgccgc
     4501 gcacccagcg cgatattgta ttcgcgggtg ccgcgctcgt ccgcatggcc ttcaatggtg
     4561 aaggaatagc ggttgtagct ctgcagccat tgcgcctgct tctccagggt cgcgatcgcc
     4621 tgcggcgaca gatcggtctg gtcgctttcg aagaacacgc ggtcgccgac attgaccacg
     4681 aagtcctgct ggctacccgg ggtcgcagca cttgccatgg cgccgtcggc gccgaggttc
     4741 ttgttcgcgc aggcgcccat tgaaagcgcc accgcgagca ccgcggccag cttcaatccc
     4801 tggaggatac gcatctgatg tttcattcgg gagcctccac gctcgcgttt ttcgactgtt
     4861 acccggtcta cagggcgatg gttaagcgaa cgttccgtca accttgatga ggtgttgcca
     4921 gacccgatca aatgccagca aataccgaaa agcaaccgcg atagttaatg agttgtaaat
     4981 gtggcgcgac ggtgaaggcg ggcaaatgcg cggtggggat aacccgtttt caggtcaacg
     5041 aaatccaccc atttcgtgca acgcgcgccg gcctcgcgca atgcgtgcct gccgagcggg
     5101 aacctgccgc ggaaaccgcc acccgcgcct attggcgcaa aactggcagg aagcgcagcc
     5161 ctgattcgtc aggaccgcgg gaggctcgcg gtgggctggg ccatcgcgcc cggcgacgat
     5221 gaatcgcgct cgccacggcg ctgaaataac attcgtcgct cgaacgcgct ggaacgcata
     5281 taggggaagc gctggaacac cctttcccga atgctcggat aatgggcagc caccagtccg
     5341 accggcttga tcaggcccgg gtacaatttc ggttcgcccc agggctcttg cagaaagacc
     5401 ttgcgataga acgcccgatg ttcggggcgg acgttggcaa gcccgatatc agcattgaaa
     5461 tggccgcacg cgatatagcc cagccgcacc gtgacatagg gaagctcggg aaacgttctc
     5521 gccttctcgg gatccgcgac gaagcgggtc ggatcgacaa tgaccttccc ccggtcgagc
     5581 tccggatgca gaagctcggt aaacatttcg gacgagggag atccccggtg ctccgaggtc
     5641 aggacgctga tgcgtataga actgtaaagc tcgcccttaa catagacgcc gaatgtgaag
     5701 ttgttgggca ggtcgtcgta acgatcactg acgcgctcgg attccgatgg caggatcgcg
     5761 ccctcacgga gatacgcgcg gtagcgcaga cggtaaatcc tgtccttgtc ctcgggcgtt
     5821 tcggcgaggc ggtagtctac gtgttcgagg ggatcggcgg ttcgttcggg cacaagggcg
     5881 attgcttcgg ccgccggcct catatgctac tccttacaaa cttcccaaca acttccgcgc
     5941 gcggcgaaga atgttaacag cttgctaacg gcaatgcaaa ggaatcgcgg cgatagggtt
     6001 aatccggcgg ctcaaattcc gcgccgcgcg cccaaaccgg tgtggaaaat cacaccgacg
     6061 aactgtcgtc atttgccgga ttttttaggc gatcgatcgc gacggcaatt cgagcaactt
     6121 ctcgtccggg aggcggcggc cgtgcgaagc gttcagcaac tggcgtatcc gcaccgccgg
     6181 caccggccgg ctgaacaaat agccttgcgc ctcggacacc gtgccgtcga cgctgatcag
     6241 ctcgagttgc tcgttggtct cgatgccttc gaccacgacc gacattccaa gatcggcact
     6301 gagccgcgcc acaccgcgca acagcgtcag cggacgatcg ctatcgatgc cctccaggaa
     6361 ggaacgatcg atcttcactt tctgcagcgg aaaattatga agataactca ggcttgaata
     6421 gccggtgccg aaatcgtcca gcgatattcg cacgccgatc atgtgcaatt gcgacagcac
     6481 gtcgtgggtc aattgcgtgt tgcgcaacag cgacgattcg gtgatctcga tttcgaggcg
     6541 gtgtgccggc aacccggaga cctcgagcgc gtaacgaacc tcgctcagca catcgcgctg
     6601 atggaactgc tgcggcgaga aattgaccgc gacgctgacg ccctcgggcc atttcatgca
     6661 ttccatgcag gccttgcgca ggatccagcg gccaagatcg acgatgaggc ccatgtcttc
     6721 ggcaaccgga atgatgtcga tcggggaaac cgtgccccgt accggatgat tccatcgcag
     6781 cagcgcttcg cacgtcgcga tccgcccgga tttgagattg acgagcggtt gatagaacag
     6841 ctcgaattcc tcgtgggcca gcgccttgcg caggtccagc tccagaatgc ggcgggcctc
     6901 gacggtttgc gccatctcgt cgcggaagaa gcagaaggtg ccgcggccgt ccgccttggc
     6961 gcgatagagc gccatgtccg cgttcttcag gagcgtatcg gcgctgatgc cggcgggcga
     7021 cgtcatcgcg atgccgacgc tggcaccgat ttcgaccaga tggttgtcga tcttgtaacg
     7081 ctcgctgagg cgatcgacaa tgcgccgggc gaggcccgcg gcgtcctcgt tggacaagat
     7141 gttctgctgg aacaccacga attcgtcgcc accgaaccgc gccacgaaat cctccgggcg
     7201 cagcatttcg cgtagtctgt cggcgacggc gcacaacagc tggtcgccac acgggtgacc
     7261 cagggtgtcg ttgacctgct tgaactgatc gagatcgaca aacagcaatg ccgacaatcg
     7321 ctcggcgctg tgcgaaatcg ccagcaggcg ctcgatttcg tcgcggaaat tgaccctgtt
     7381 gggcagcgcg gtcagttcgt cgtaacgggc gagatggctg atcctggctt ccgcattctt
     7441 ccgctcggta atgtcttcca ccagcacaac ggtgccgccg ccggccattg attgaaacgt
     7501 ccaggccagc gaccggccgc tcgctggact ggagtcggtg gtgatgatgt ccttggcctg
     7561 tgaattgccg atttccgaga gaatcatcct tccgctcgcg gccgagagcg acccggcgct
     7621 aacgcacgcg tcgacaatat cgggcgcgcc ggcgccgcga tgcacgagat cgtcgcgcag
     7681 gttcatcatg gcagtgaagc gatgattgac gacggcaagc cggccgtctc cgcgaaacat
     7741 gcacaggccg tgcggcatgt tgttcaaggc ggtgtcgaac tggctcgcca gtgcggcctc
     7801 gcgctcgcgc gcaaccagcg cccgtacgaa aatcttctgc aggctggtcg agatgtgttt
     7861 gagggcgagg aagaacagta cgttcaggac cgccatcccg aaatagtacg gacttccata
     7921 cagagccagc gccagcgtca gcggcccgca ggccagcagc atttgcacat gaaagatcca
     7981 cggacgcccg taggttcgac agcccccggc cgccacgtag caaagggtga ccgagatggc
     8041 gatcatgtgt gcgacggcat cgctgctccc cagcaccgtc accatgcacc atagcccgag
     8101 cgccgccgca tagagcatcg caccgaactg gtagcggact tcccagcgtg ccgcttcgtt
     8161 cgacgtcaga ccgcaattgc gctgccgata ttgacgcatg tcgagcgcac ggacggcgcc
     8221 ggtcacaacc agaagcgcga cgctgggcca aagccatggg ttgccggtct tcacggccgt
     8281 catgatcgcc ccggccgcgg cgcaaagcgc gccagcaaac agcggaccag gatcctggaa
     8341 taacgaatcg atcagcgccg catagatcga cggcggcatc ttctcgtcgg ggtctccggt
     8401 ctcgcttgca agctgcatcg gatgcgcgca cgtcctcttg ggctgcgcag ttttaacctc
     8461 tggcagatga agtctttctg agggaacatc gttagcggaa agttgttttt ggccccgaaa
     8521 caaaaatctg ctcgataaat cagcaggcta ggcaagcttt gccgaccaag ggaccacccc
     8581 cggcgatggc ggtgccaatc ctcaagacaa taggggcgac caggccggat cggacgcgaa
     8641 gccgggggtg ggaacccgtt gttcattgcg tccggaaacg tccacggtga acagcgaagg
     8701 cccgccactg ccgccggggt cgcggaagaa catcaaaacc ctgccgttcg gcgcgaaggt
     8761 aggtccctcg ttgtggaatc cgctggtgag gatccgctcg cccgaaccgt cggtcttcat
     8821 gatgccaatg gaaaactgac cgccgccctg cttggtgaag gcgatatagt cgccccgcgg
     8881 cgaccacacc ggagtggaat agctgccttc gccaaaggag atgcgttgcg caggcccccc
     8941 ggttgccggc atcacataga tctgcggctt tccgccacgg tcggattcga aacacaggcg
     9001 ggtcccgtcg ggcgcataag atggtgaggt atcgatcgcg ggggtgtcgg tcagacgcgt
     9061 ggtcgatttc gagcggagat ccatcacgaa caggttcgaa ttgccgccct gctgcaggct
     9121 catgatcacg cgctggccat ccggcgagaa ccgcggcgag aacgacatgc cgggaaaatt
     9181 gccgacgatt tcgcgctcac cggtctcgat tcgatacaag tagacccgcg ggtcgccctg
     9241 gccgaattcc atataggtga tcagctgctc gtcgcgcgcg ggcgaaaacc gcggcgtcag
     9301 caccagatcg gcgcccttgg tcagatagcg aacattggcg ccgtcctgat ccatcagcgc
     9361 cagcttcttg acgcgccgct ccttcggtcc ggattcgtcg acgaacacca cgcggctgtc
     9421 gaaatagcct ttgtcgccga tcagatgctc gtagatctgg tcggaaatga tgtgcgcgat
     9481 cctgcgccaa tattccggcg aggtgaaata ttgctggccg gtgatctgct ggcccgtggt
     9541 gacgtcccac aagcggaatt ccgcctttag acgtccgtcg ggctgccggg tcatgcgacc
     9601 ggtcaccagc gcctgggcgt tgatggtctt ccagttctgg aattgcggag cggcatcgat
     9661 gttaatggtt ttttcgatat aggccgcctg atcgatcggg gcgaacagtc cgctgcgcct
     9721 gaggttgttg gtgatgacct gggctacatt caccccggct tcgccgtccg atggcgttcc
     9781 cgccgcgaaa ttcggtatcg cgatcgggat cgcctgggtg ccgccgggta cgacttcaag
     9841 tcgctgctgc ccctgcgcgg cgagtccacc cggacgcatc attcctgaca ccgccaacgc
     9901 gccagccgaa aatgtgaaag cccgccggga catcggtgac gacagacccg gcgccggatt
     9961 gccgcggaac aaatcaaaca tgctagtaag ccttgttatg gaacaagaag ggaagcacct
    10021 tccactcggc atatttcgcc tgcggcagca tggtgtatgg ctggcaagca atgatagccc
    10081 gacgggcccc ttcgaaaatt gcacgctcgc ggatattgcg tggctcgatt tcgatcaacg
    10141 gcgccgctga aagcgtaccg tcgactttaa acgtaagcgt gatcggaatc ttcagatcgt
    10201 cttcatcgaa cccgccgccg tcgcgctgcc agcaatcatg aatcctgctg acaagcgcgg
    10261 aaaggtaact cgctgatggc gttgccgcgg tccccttggc cgttccgagc gccgcggtcg
    10321 agttcagcgc gtcgccggca aacgcctgtc gggacggagc ctgtttgtcg accagcgccg
    10381 agatggattc cctgtcgaat acatattcgc gtttcttgac cggtggcggc ggcgccgcct
    10441 tggctgccgg cttcggtgcc ggcttcttgg cttcttcttt tttcagagcc tccgcgatcg
    10501 aatcgacctt gggcggatcc ggcttctttt cgatcggctt cggttcatcc ttgggtttgg
    10561 tctcggcgac cggcttcggc ggatccggcg ttttctcgac gggtttttcc accggcttcg
    10621 gcgtcggatc gggggcggcg tcggtcacga ccggtttctt ttcggtaatc ttgccgacgg
    10681 cgtcatccat cggcttggct tcggcgacct tctccaccag cggcttcgga ttttcctttt
    10741 taccggattt gatgccggcc gtaatcttgg acagttcgtt gtccgtcacg aattcaacag
    10801 gaacggactc gatctcggtc gactcaaatg ccctggacga aaacgacacc agtccccagc
    10861 cgagcacgac gacgtgcaag gccacggacg caacaagtgt cttgtcgacc ttcaccttca
    10921 ggacccctgc tccacctcgg tcaccagcgc cagccgcttg aagccggcgc ccgacaactg
    10981 tcccatgatc ctcgccacgg ttccgtaatc cgccttcttg tccgcccgca tgaatatacg
    11041 ctcgtccatg ccaccacggg cgtccgtgat cgcctttaat ttcggtatca gctcgttcat
    11101 cgccacttcg gtattattga tgaacacctt tccctgaaca tcgactgaaa gctgcagcgg
    11161 agtcttgtcc tgatccaggc tcttggcctg ggtctgcggc agatcgagtg gcacgccgac
    11221 cgtcagcagc ggcgccgaca ccatgaagat gatcagcaac accagcatta cgtccaccat
    11281 cggcgtgacg ttgatctccg ccatcactgc tttgcgccgg ccgcggcgac cgccgccgcc
    11341 cgacgagccc gccatgttca tcgccatgat ctggttctca taagggggct catagcatat
    11401 cgcgcctcat gcccgctcgt cgatctggcg cgacaggatt gctgaaaatt catcggcgaa
    11461 gccttccagc cgctgcgcct gccggttcac ctcggaggtg aacttattgt agaagatagt
    11521 ggccggaatg gcggcgataa ggccgatagc cgttgcaaac agggcctccg cgatgcccgg
    11581 ggccaccacg gccagcgagg tgtttttcga ggcggcgatc gactggaagc tcgacatgat
    11641 gccccacacc gtgccgaaca ggccgacaaa cgggcccgcc gagccgaccg tcgccagcac
    11701 cagcaggcga cgttcgagcc gctcgatttc ccgggcgatg gagacgttca tgaccttctc
    11761 gatccgcatt tgaagcccgg cgaaggatcg cgcctggctt tcgaacgacc gtttccattc
    11821 gcgcatcgcc gcgacgaaac aagccgccat cgactgcgtc ggcttggcgg agagcgcgcg
    11881 ataaagttcc tcgatcgact gcccggacca gaacgcctgt tcgaagcgat ccatcgcgcg
    11941 cttggtgcgc gcgtaaagga acgtcttgtc gatcgcgatc gcccacaccc agaccgaaca
    12001 ggccagcagc ccaagcatga ccgctttcac gatccagtga gcctgcagga acagtgcgat
    12061 cagcgatacg tcggtggatg ccagcggcaa tgctgactgg gccacgtcgg ccggattcat
    12121 aagcggtatc ctctcaacgc aagcgtccca atcctccggg agcaaatggc tgccggttcc
    12181 acagccgcgc gtcgggcgcg gcggtgagcg ccaacgcaga cgccttgttg tgtcggatgg
    12241 g
//
LOCUS       DATNOI010000120         3097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2322296,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000120 DATNOI010000000
VERSION     DATNOI010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2322296"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VN968_04450"
     CDS             complement(<1..407)
                     /locus_tag="VN968_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL28525.1"
                     /translation="MAGAQLAAKIETAAKAHDSSRRTAPHVTIRGLTKRFSDATIYDN
                     FDFDIPRGKLTSVFGPNGCGKSTLINMIAGLFPVDAGEILFDGQPLNQIKFGYVFQNY
                     REAMFPWLRAFDNIAYPLKVMGVPKAVRNARVE"
     gene            complement(420..1394)
                     /locus_tag="VN968_04455"
     CDS             complement(420..1394)
                     /locus_tag="VN968_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL28526.1"
                     /translation="MKLLNFVMGAVAALALSVAGPGRAAAQDKVSVGVFPVSSSLPYF
                     VALERGFFKEQNIEPEMTRLMGGPPNVAAMMTNQIEVSAVLVTLEGLNANVKKPGVAM
                     YIALNSQTKVWKMEQFVVRNGFKADTIADLKGAKLMSAPGPANLNTAKAILGKNGLKE
                     GDYTIDQLDMGQHINAMTAGTFDGGYTLEPNASMMIKAGVARSLEAGVISKYILGDEA
                     ADAYAAGCAMTSDFIGKRPEVAKRFAAAWGKAIDFINKNPDEARKYLAKNTFTPDNVV
                     DVVSMLGYVMAGDMTQKQIGDLQKLADFGNSIGVVPEKLDVTKVLQKF"
     gene            complement(1481..2575)
                     /locus_tag="VN968_04460"
     CDS             complement(1481..2575)
                     /locus_tag="VN968_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-opening amidohydrolase"
                     /protein_id="HXL28527.1"
                     /translation="MAIEVAKVELKTVQDASGLEGLIAAGKFGADEVIAVIGKTEGNG
                     GVNDFTRILADQAFRRVLLKHGTRSEAEIHNVPMVWSGGCDGVITPHATIFARNSKIG
                     PADKSRLVIGTALSAELLPEDIGRPAMVLKVADAVKAAMKDAGITDPKDVHYVQTKTP
                     LLTIDSVRDAETRHQTLACEVHDSMGVSNGTTALGIAVALGEVPMPQAEQICKDLDLY
                     SSVASCSSGVELTQAQIVLLGNKAGAGGPYRIGHSVMRDALDIDGIYDSIRDAGIELP
                     ERPRSEDLKGRVVNCFMKCEADRRGVLRGRRQIMLDDSDVHHHRHSKAAVGGVAALAI
                     GDPAVFVSVDAMHQGPHGGGPVIAIVELDE"
     gene            complement(2624..>3097)
                     /locus_tag="VN968_04465"
     CDS             complement(2624..>3097)
                     /locus_tag="VN968_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HXL28528.1"
                     /translation="HYRALVDCRTIVTADRVRLGCYGLNGGKAGQPFCVTVDAEGEAR
                     DLGGLVDGEAVLKGQVVRVVTTGGGGWGDPLQRELEFVLRDAIEGRVSPASAEADYGV
                     VFAAASTADQLKIDEAATKARRADIEKARAAPPGMIDRGEGYEKMLRGEHRPRMR"
BASE COUNT          533 a         1024 c          913 g          627 t
ORIGIN      
        1 ttctccaccc gcgcgttgcg cacggccttc ggcaccccca tcaccttgag cggatacgcg
       61 atattgtcga acgcgcgcag ccatggaaac atcgcctcgc ggtagttctg gaagacgtag
      121 ccgaacttga tttggttcaa cggctggccg tcgaacagga tttcaccggc atcgaccgga
      181 aacagccccg cgatcatgtt gatcagcgtg ctcttgccgc agccgttggg accgaacaca
      241 ctcgtcagct tgccgcgcgg aatatcgaaa tcgaaattgt cgtagatcgt ggcgtcggaa
      301 aaccgcttgg tcagcccgcg gatcgtcacg tggggggcgg ttcgccgcga cgaatcgtgg
      361 gctttggccg ccgtttcgat cttcgcagct agctgggctc cggccacttg ggtgcgcttt
      421 cagaatttct gcaggacttt ggtgacgtcg agcttttcgg ggacgacgcc gatcgaattg
      481 ccgaagtccg ccaatttctg caaatcgccg atctgttttt gggtcatatc gccggccatg
      541 acatagccga gcatcgaaac cacatcgacc acgttgtcgg gcgtgaacgt gtttttcgcg
      601 agatatttgc gcgcctcgtc agggttcttg ttgatgaaat cgatcgcctt gccccacgcc
      661 gcggcaaagc gttttgcgac ttccggcctc ttgccgatga aatcgctggt catggcgcag
      721 ccggccgcat aggcgtcggc ggcctcgtcg cccaggatat atttcgagat cacgccggct
      781 tcgagcgaac gcgcgacgcc ggccttgatc atcatcgagg cgttgggctc aagcgtgtac
      841 ccaccatcga acgtgccggc ggtcatggcg ttgatgtgct ggcccatgtc gagctggtcg
      901 atggtgtagt cgccctcctt caggccgttc ttgccaagaa tggccttggc ggtattgagg
      961 ttggcgggcc ccggcgccga catcagtttt gcgcccttga ggtcggcgat ggtgtcggcc
     1021 ttgaaaccgt tgcggacgac gaattgttcc atcttccaga ccttggtctg gctgttgagt
     1081 gcgatataca tcgccacgcc gggcttcttc acgttggcat tgaggccttc aagtgtgacg
     1141 agcaccgccg agacctcgat ctggttggtc atcatcgccg ccacattcgg cggcccgccc
     1201 atcagcctgg tcatctcagg ctcgatgttc tgctccttga agaagccgcg ctccagcgcg
     1261 acgaaatagg gcagcgacga actgacggga aataccccga ccgacacctt gtcctgggct
     1321 gcggcccgac ccggcccggc cacgctcagg gccagcgccg cgacagcgcc cattacgaaa
     1381 ttcagcagtt tcatcgtccg ctcccgtcat ccaccttacg agcacctgcc ggtcaggcgt
     1441 ccggcagaac actcatgttc gagcaaggaa cgtgccggtt ttattcgtcc agttcaacga
     1501 tcgcgatcac cggaccgccg ccatgcggtc cctgatgcat ggcatcgacc gatacaaata
     1561 ccgcggggtc tccgatcgca agcgcggcca cgccgcctac cgccgccttc gaatgccggt
     1621 gatggtggac gtcggaatca tccagcatga tctgccgccg cccgcgcagc acgcccctgc
     1681 ggtccgcctc gcatttcatg aagcaattga cgacacggcc cttgaggtct tccgaccgcg
     1741 gccgttccgg cagctcgatc ccggcatcgc gaatcgagtc gtagatgccg tcgatatcaa
     1801 gcgcatcacg catgaccgaa tggccgatcc ggtaagggcc gccggcgccg gccttgttgc
     1861 cgagcaggac gatctgagcc tgagtcaatt ccacgcccga cgaacaggaa gcgaccgacg
     1921 agtacaggtc gagatcctta cagatctgtt cggcctgggg catcggcact tcgccgagcg
     1981 ccacggcgat gccgagcgcg gtggtgccgt tggaaacgcc catggagtcg tggacctcgc
     2041 aggccagcgt ctggtgacgc gtttcggcgt cgcgaacgga gtcgatggtc aacagcggcg
     2101 tcttggtctg gacgtaatgc acgtccttcg gatctgtaat cccggcatcc ttcatcgcag
     2161 ccttgacggc gtcagcgacc ttcaaaacca tcgcgggacg accgatatcc tcgggcagca
     2221 attccgcgct cagcgccgtg ccgatcacaa ggcgcgattt gtcggccgga ccgatcttgc
     2281 tgttgcgcgc gaaaatcgtg gcgtggggcg tgatgacacc gtcgcagccg ccggaccaga
     2341 ccatcggcac gttgtgaatt tcggcttccg agcgcgtgcc gtgcttgagc aacacgcgcc
     2401 ggaacgcctg gtcggccaga atgcgggtga aatcgttgac gccgccattg ccttcggtct
     2461 tgccgatcac cgcgatcact tcgtcggcgc cgaacttgcc ggccgcgatc aatccctcga
     2521 ggccactcgc gtcctgcacg gtcttcaatt cgaccttcgc aacttcaatc gccattcgcc
     2581 atccactccg ctgtcccttg cccaggtttc atcttccaaa gtttcatcgc atccgcggcc
     2641 ggtgctcgcc gcgcagcatt ttttcatagc cctcgccgcg gtcaatcatc ccgggcggag
     2701 cggctcgcgc cttctctata tcggcccgcc gcgcctttgt cgccgcttca tctattttca
     2761 gctggtccgc ggtgctcgct gccgcaaata ccacgccgta gtcggcctcg gcgctggcgg
     2821 gcgaaactcg cccctcgatt gcgtcgcgca gcacgaattc gagttcgcgc tgcagcggat
     2881 cgccccagcc gccgccgccg gtggtcacca cccgcaccac ctggcctttg agcaccgctt
     2941 cgccatcgac cagcccgcca aggtcgcgcg cttcgccttc ggcatcgacg gtgacgcaga
     3001 acggctggcc ggccttgccg ccattgaggc cgtaacagcc cagccgcacg cgatcggcgg
     3061 tcacgatggt gcggcaatcg accagcgcgc gatagtg
//
LOCUS       DATNOI010000121         5610 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_650447,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000121 DATNOI010000000
VERSION     DATNOI010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650447"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VN968_04470"
     CDS             complement(<1..368)
                     /locus_tag="VN968_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HXL28529.1"
                     /translation="MSASVLTTDLFASIPDLRPTQVLMAEGAVLLHGFAKPVEAELIA
                     DLRDIVEQAPFRHMVTPGGHQMSVAMTNCGGRGWVTDRSGYRYDANDPETGKPWPAMP
                     RSFRELAGQAADHAGFGGFSP"
     gene            complement(365..1111)
                     /locus_tag="VN968_04475"
     CDS             complement(365..1111)
                     /locus_tag="VN968_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HXL28530.1"
                     /translation="MKSTAKNIVPAVPSDIAGRVDAIDWAQATSDLDAQGCAMSKGLL
                     SRDECRALVALYPDDKHFRSRIVMGRHGFGRGEYKYFSYPLPHLIAELRPALYARLRD
                     TANRWNEAMGIDVRYPETHEAFLKRCHAAGQTRPTPLLLQYGASDYNCLHQDLYGEHV
                     FPLQVAILLSEPGRDFTGGEFVLTEQRPRMQSRPEVVPLAQGDAVAFAVHHRPIQGTR
                     GFYRVNLRHGVSRIRSGHRHTLGVIFHDAK"
     gene            complement(1261..1947)
                     /locus_tag="VN968_04480"
     CDS             complement(1261..1947)
                     /locus_tag="VN968_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HXL28531.1"
                     /translation="MFDLTFTIDAQDTTTPLTLSIDRAVMAGWTGRDPVARDKHIAEL
                     EAIGIARPATTPIYYRVAARRLTTEDSIEVSGGDTSGEVEFVLVGWQGRIFVGVGSDH
                     TDRKVESYGITVSKQMCDKPIAPVLWELEDVAGHWDKMVLRSHALIGGTRELYQEGTL
                     DNLLSVEELIDRGFGGGGLPDGCAVFGGTFAAKGGIRPASRFDFELVDPVLKRSIRHA
                     YDVIELPVLG"
     gene            complement(1940..3289)
                     /locus_tag="VN968_04485"
     CDS             complement(1940..3289)
                     /locus_tag="VN968_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXL28532.1"
                     /translation="MPNTPTLTSLTDDLENGRTSARKLVDECLVKIADSSGEGARAFL
                     HVDAEAAIEAAEAMDRLREVKAAPSRFAGIPVSIKDLFDIKGQVTRAGSRALDDSAPA
                     EADAPVVARLRRAGFIAIGRTNMTEFAYSGIGINPHYGTPKSAWRRDVGHVPGGSSSG
                     AAVSVVDRMAHGALGTDTGGSCRIPAAYNGIVGFKPTQRRVPLDGGVPLSFTLDSFGP
                     LARSVGCCAVLDAVLADEPIVPPQPRPIKGMRLAVPTTVALDELDDEVARTFERVLER
                     LSRQGALIERIAVPEFLDVGVMNTKGGFAAAESYAWHRYLIVSHGDVYDPRVYTRILR
                     GEAIGAADYIDLLKARKSLIARATARLAPYDALVLPTTANTPPRIADLADDKAFTKAN
                     LLSLRNCTLINMIDGCAISLPCHREGEVPVGLMLAASGGADRRIFELAAAMEDVIRV"
     gene            complement(3318..3638)
                     /locus_tag="VN968_04490"
     CDS             complement(3318..3638)
                     /locus_tag="VN968_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28533.1"
                     /translation="MKLREIAHSRTGDKGNTSNISVIAYDEKYYPLLLAQVTSARVKA
                     HFAGVVEGEVVRYELPNIAALNFVMTQTLGGGVTHSLALDAHGKSLSSALLDLEIEPA
                     SDNG"
     gene            complement(3643..4983)
                     /locus_tag="VN968_04495"
     CDS             complement(3643..4983)
                     /locus_tag="VN968_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HXL28534.1"
                     /translation="MRTIRIGSGAGYSGDRIEPAVELAEKGDVQYLVFECLGERTVAL
                     AQQARMKDPDSGYDPLLEERMRAVLPSCAARGIKIVTNMGAANPAAAARKTAEIATSL
                     GLPSLKIAAIIGDDVLDACKERDLKIMEFDGSIKQLGNRLLSANAYLGAEPIAQALSG
                     GADIVITGRASDPALFLATMIHAFGWAMDDWNLLGQGTVAGHLLECAGQITGGYFADP
                     GYKDIPDLARLGFPIGEVGEDGALVITKVAGSGGAVTAQTCKEQLLYEVHDPARYLQP
                     DVTADFSQVRVEEVARDRVRVSGGRGTERPATLKVSVGYVDSYIGEGQISYAGPGSLA
                     RGRLALEIVRERLKLTGVAAGELRFDLIGVDALHGANLAAHASEPYEVRVRVAGRTEN
                     LREAVRIGNEVETLYTNGPAAGGGVFKSARDVVAVASVLLPRELARPQIQFVGG"
     gene            5188..5598
                     /locus_tag="VN968_04500"
     CDS             5188..5598
                     /locus_tag="VN968_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HXL28535.1"
                     /translation="MAEPARARPKPTPETQHFWDGTKAGELRLQRCDACANVYFPPRP
                     FCPVCASRKVSVFKASGKAKLYSYVIHHRPVPGFTPPYAIAVVELDEGPRMMSNIVDC
                     PQTPEALELDMKLEVAFEKLDDNITLPLFRPAKG"
BASE COUNT         1025 a         1822 c         1775 g          988 t
ORIGIN      
        1 ggtgagaagc cgccgaaacc agcatgatcg gccgcctgcc cggcgagctc gcgaaacgat
       61 cgcggcatcg ccggccacgg cttgcccgtt tcgggatcgt tggcatcata gcgataaccg
      121 ctgcgatcgg tgacccagcc acgaccgccg caattggtca tcgccaccga catctgatga
      181 ccgccgggcg taaccatgtg gcgaaacggc gcctgctcca cgatatcgcg caggtcagcg
      241 atcagctcgg cttcgaccgg ttttgcaaat ccatgcagca gcacggcgcc ctctgccatt
      301 agcacctgcg tcggacgcaa atcgggaatg ctggcaaaca aatcggtcgt caaaacactc
      361 gcactcactt cgcatcgtga aagatcacgc caagggtatg acggtggccg gagcggatcc
      421 ggctcacgcc gtggcgcagg ttaacccggt agaatccccg cgtcccctgt atcggcctgt
      481 gatgcacggc aaaagccacc gcatcgcctt gcgcgagcgg caccacttcg ggccgcgact
      541 gcattcgtgg ccgctgttcg gtcagcacga attcgccgcc ggtgaaatcg cggcccggct
      601 ccgacagcag gatcgcgacc tgcagcggga acacgtgttc gccgtagagg tcctgatgca
      661 ggcagttgta gtcactggcg ccatattgca gcaatagcgg cgtcggccgg gtctggccgg
      721 cggcatggca gcgtttcagg aaggcttcgt gggtctcggg atagcgaacg tcgatgccca
      781 tagcctcgtt ccagcgattg gcagtatcgc ggagccgcgc gtaaagcgcc ggccgcaatt
      841 ccgcgatcag gtgcggaagc ggataggaaa aatatttgta ctcgccgcgc ccgaagccgt
      901 ggcgtcccat cacgatccgg ctgcggaaat gcttgtcgtc cggatagagc gccacaagcg
      961 cgcggcactc atctcgcgac agcaatccct tcgacatggc gcagccctgc gcatcgaggt
     1021 cgctggtcgc ctgcgcccag tcgatcgcat cgacgcgccc ggcgatatcg gagggaactg
     1081 cagggacgat attttttgcg gttgatttca tggctgcgat tttcgcaaat cggatcgttt
     1141 tgcgccaccc gatttccggg taaaggcccg ccgtcattgc gagcgtagcg aagcaatcca
     1201 cgcttcgccg caaagaaagc ctggattgct tcgtcgctga cgctcctcgc aatgacggaa
     1261 ctaacccagc accggcagct cgatcacatc ataggcgtgg cgaatgctgc gcttgagaac
     1321 ggggtccacg agttcaaagt cgaagcggct cgccgggcga atgccgccct tggccgcaaa
     1381 agtgccgccg aacacggcgc agccgtccgg caatcctccg cctccgaaac cacgatcaat
     1441 gagttcttcc accgaaagca ggttatcgag cgtgccttcc tggtacagct cgcgcgtgcc
     1501 gccgatcaag gcgtgcgaac gcaggaccat tttgtcccag tgaccggcga cgtcttcgag
     1561 ttcccacagc accggcgcga tcggcttgtc gcacatctgc tttgacaccg tgatgccata
     1621 gctttcgacc tttcgatcgg tgtgatcgga accgacaccg acaaagatgc ggccctgcca
     1681 gccgaccaag acgaattcca cctcgccgct ggtgtcaccg ccgctgactt cgatgctgtc
     1741 ctccgtggtc agccgccgcg cggcgacccg gtaatagatc ggggtagtcg ccggccgcgc
     1801 gatgccgatc gcttcgagct cggcgatgtg cttgtcgcgc gcgaccggat cgcggccggt
     1861 ccagccggcc atgactgccc gatcgatcga aagagtcagc ggcgtcgtgg tgtcctgggc
     1921 gtcgatggtg aatgtgagat caaacacgga tgacatcctc cattgcggcc gcgagttcga
     1981 agatgcggcg gtccgctccg cccgatgccg caagcatcag cccgaccggc acctcgccct
     2041 cgcgatggca gggcaacgaa atcgcgcagc cgtcgatcat gttgatcagg gtgcaattgc
     2101 gcagcgacag caggttggcc ttggtgaaag ccttgtcgtc ggcaagatcg gcgatgcgtg
     2161 gcggcgtgtt ggcggtagtc ggcagaacca gcgcgtcata gggcgcgagg cgagcagtgg
     2221 cgcgcgcgat cagcgacttc cgcgccttga gcaaatcgat atagtccgcg gcgccgatgg
     2281 cttcgccgcg aaggatgcgc gtgtagacgc gggggtcata gacgtcgcca tggctgacga
     2341 tcagatagcg atgccaggca taactttctg cggctgcaaa accgcccttg gtgttcatca
     2401 cgcccacatc gaggaattcc ggcacggcga tgcgctcgat cagcgcgccc tggcgcgaca
     2461 gcctttccag cacgcgctcg aaggtccgcg ccacttcatc gtcgagttcg tcgagcgcga
     2521 ccgtggtcgg cactgcgaga cgcatgccct tgatcggtcg cggctgggga gggacgatcg
     2581 gctcatcggc gagtacggcg tcgagcaccg cgcagcatcc aacgctgcgc gcgagcgggc
     2641 cgaaactatc gagcgtgaac gagagcggca cgccgccgtc gagcggtacg cgacgctgcg
     2701 tcggcttgaa gccgacgatg ccgttgtagg cggccggtat ccggcacgag ccgccggtat
     2761 cggtgccgag cgcgccatgc gccatgcgat cgacgactga caccgccgcg cccgaggacg
     2821 atccgccggg cacatgcccc acatcgcgcc gccatgcgct cttcggcgtg ccgtaatgcg
     2881 ggttgatgcc gatcccggaa taagcgaact cggtcatatt ggtccggccg atcgcgatga
     2941 aaccggcacg gcgcagccgc gctaccacgg gcgcatcagc ctccgccggg gctgaatcgt
     3001 ccagcgcgcg cgagccggcg cgggtcacct gccccttgat atcgaacaga tccttgatcg
     3061 agacgggaat gccggcaaag cgcgacggcg ccgctttcac ctcgcgcagc cggtccatgg
     3121 cttccgccgc ctcgatcgcg gcttccgcat cgacgtgcag gaaggcgcga gcgccctcgc
     3181 ccgagctgtc ggcgatcttc accaggcact cgtcgacaag cttgcgggcg ctggtgcggc
     3241 cgttttcgag atcatcggtc agtgatgtaa gtgtcggcgt gttcggcatt gcgttgctgc
     3301 ttgtccctgt tgatggatta tccgttgtcg gaagcgggct cgatctccag atcgagcagc
     3361 gcggagctca gggatttgcc gtgggcgtcg agcgccagcg agtgtgttac gccaccgccg
     3421 agcgtctggg tcatcacaaa attcagcgcg gcaatgttgg gcagctcata gcgcacgacc
     3481 tcgccttcga ccacgccggc aaaatgcgct ttgacgcgcg cgctggtgac ttgcgcaagc
     3541 agcagcggat agtacttttc gtcgtaggcg attaccgaga tgttggaggt gttgcccttg
     3601 tcgccggtgc gggaatgggc gatttcgcgc agcttcatcg cctcaacctc ccacgaactg
     3661 gatctgcggc ctggcgagtt cgcgtggcag caggaccgac gccaccgcga ccacatcgcg
     3721 tgccgatttg aaaacaccgc cgccggcggc cgggccattg gtgtagagcg tttcgacttc
     3781 gttgccgatc cgcacagcct cgcgcagatt ttcggtgcgg ccggcaacgc gaacgcgcac
     3841 ctcgtaaggt tcgctcgcgt gggcggcaag gttggcgccg tgcagcgcat cgacgccgat
     3901 caggtcgaac cgcaattcac ctgcggcgac gcccgttagt ttcaaccgtt cccgaacgat
     3961 ctccagcgcc agccgtccgc gcgccagcga gcccgggccg gcataggaaa tctggccctc
     4021 gccgatatag ctgtcgacat agccgaccga aaccttcagc gtagcggggc gctcggtgcc
     4081 gcgtccgccg ctgacgcgta cgcgatcccg agcgacttcc tcgactctta cttgcgaaaa
     4141 atccgccgtt acgtcgggct ggagataccg tgcgggatcg tgcacctcgt agagcagttg
     4201 ctccttgcaa gtctgcgcgg tcaccgcgcc gcccgagccc gcgaccttgg tgatgacgag
     4261 cgcgccatcc tcgccgacct cgccgattgg aaagccgagc cgcgccagat cggggatatc
     4321 cttgtagccg gggtcggcaa aatagccgcc ggtgatttgg ccggcgcatt cgagcaggtg
     4381 gccggcgacg gtgccctggc ccagcaaatt ccagtcatcc atcgcccagc cgaaggcgtg
     4441 gatcatggtc gccagaaaca gcgcgggatc ggaggcgcgc ccggtgatca caatgtcggc
     4501 gccgccgctc agggcttgcg cgatcggctc ggcgccgaga taggcgttgg ccgacagcag
     4561 ccggttgccg agctgcttga tcgagccgtc gaattccatg atcttgagat cgcgctcctt
     4621 gcaggcatcg agcacgtcgt caccgatgat ggccgcgatc ttcagtgacg gcaggccgag
     4681 ggaagtggcg atctccgccg tcttgcgggc tgccgcggcc gggttggccg cgcccatatt
     4741 ggtgacgatc ttgatgcccc tcgcggcgca gctcggcagc accgcgcgca tccgctcctc
     4801 gagcagcgga tcgtaaccgc tgtcgggatc tttcatgcgt gcctgctgcg ccagcgccac
     4861 ggtgcgctca ccgagacact cgaataccag atactggacg tcgccctttt cggcgagttc
     4921 gaccgcgggc tcgatgcgat cgcccgaata gccggcaccg gatcctattc gtatcgttcg
     4981 cacaccatgt cctccggcgc cgcgtcactt ctttctagcg cgggaaatcg agagcataca
     5041 agcaccccag gccagcgatt gtgcggatgc cggaatatgc gccatgcggc agggtcgctc
     5101 gaccgttgac ggcggatggt caaatgtcgc atcaagattg gaacaagcgg ccagatggcc
     5161 cgaccccgcc gggaggaaat gaccgacatg gctgagccag cacgcgcgcg accgaaaccg
     5221 acaccggaaa cccagcattt ctgggatggc accaaggcgg gtgaattgcg cctgcagcgc
     5281 tgcgacgcct gcgccaacgt ctatttcccg ccgcgcccgt tctgcccggt ctgcgcctcg
     5341 cgcaaggtca gcgtgttcaa ggccagcggc aaggcaaaac tttacagcta cgtgattcat
     5401 caccgcccgg tgcccggctt cacgccgcct tacgccatcg cggtggtgga actggacgag
     5461 ggcccgcgca tgatgagcaa catcgtcgat tgtccgcaaa cgccggaggc gctcgaactc
     5521 gacatgaaac tggaagtcgc gttcgaaaag ctcgacgaca acatcaccct tcccctgttc
     5581 cgcccggcga agggttaagc atcatgcgaa
//
LOCUS       DATNOI010000122         4296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1185476,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000122 DATNOI010000000
VERSION     DATNOI010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185476"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VN968_04505"
     CDS             <1..910
                     /locus_tag="VN968_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HXL28536.1"
                     /translation="PLDLGVRALNTFVTCCRGQRLGIFAGSGVGKSVLLSMLARNVDA
                     DITVIGLVGERGREVQEFLQDDLGEEGLARSVVVVATSDEPALMRRQAAYLTLAIAEY
                     FRDEDKDVLCLMDSVTRFAMSQREIGLSAGEPPTAKGYTPTVFTELPRLLERAGPGIG
                     AGTITGIFTVLVDGDDHNEPIADAVRGILDGHVVMERGIAERGRFPAINILKSVSRTM
                     PRSADPVYLPVIAKGRQVMATYSDMEELIRLGAYRAGSSAEVDEAIALREPLEAFLRQ
                     DKDEVTGLTDGYRQLEQILGSLETER"
     gene            1020..1439
                     /gene="fliJ"
                     /locus_tag="VN968_04510"
     CDS             1020..1439
                     /gene="fliJ"
                     /locus_tag="VN968_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HXL28537.1"
                     /translation="MKSRETLIRLKKFQVDEKRRRVAQIEGMIADFQRMSVDLEREIH
                     SEQERAGINDPSHFAYPTYAKAAIQRRENLTRSTDELRIQLEDAKSLLTEAFEELKKV
                     ELLDERDQARERAEESAREQADLDSIGLMRARIGAIA"
     gene            1639..2184
                     /locus_tag="VN968_04515"
     CDS             1639..2184
                     /locus_tag="VN968_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXL28538.1"
                     /translation="MLTPAELVWLIAAVAKGDEAAFERLYAATRAKLFGVVLRILRRQ
                     DLAEEVIQETYVKIWNSAGQFNPGQSSPITWMASIARNRAIDVVRKRGELSIEEEPAA
                     MEVAADSPDPLARREMSEELKRLLECVGRLEPDRQKLVLLAYYNGWSREQLAAKFETP
                     VNTVKTWLRRSMMDIRECLGL"
     gene            2188..3045
                     /locus_tag="VN968_04520"
     CDS             2188..3045
                     /locus_tag="VN968_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HXL28539.1"
                     /translation="MAYSEDDIALAAEYALGTLDADERAEAETKMSVDKDFAAMVEAW
                     QHKLGVLNQMVGSVEPRPELWNKIKTAIGLSEAHAALTLPATPPAASVAVEPAAVADT
                     SSVVQLSTRARRWRNVATFATAIAAALIAMIAVGVYQPDLLPGAIRPKPRTQVVEVRT
                     PSAPTPRSAQYVAVLQKESGAPAFILTVDAATKSFTVRKVGASAEPGKSFELWLISDK
                     LPRPRSLGVIGNSDFTARPVLASYDSDVINNATYAVTLEQAGGSPDGVPHSAPVFTGK
                     LIETVPPAR"
     gene            complement(3188..3385)
                     /locus_tag="VN968_04525"
     CDS             complement(3188..3385)
                     /locus_tag="VN968_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28540.1"
                     /translation="MNHSIYSADRSTHLKIVVVALVAGIAVAAFGISARTNSDDYTQT
                     ARVMKAGKAVTVTSTNSAVVR"
     gene            complement(3691..>4296)
                     /locus_tag="VN968_04530"
     CDS             complement(3691..>4296)
                     /locus_tag="VN968_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HXL28541.1"
                     /translation="ELLKNNMSDLLSYGEVQKLLKDLPKEQGELVKDIVPSQVTISGI
                     QRVLQLLLAERISIRDLSTILEGIADALAFSRNPATMVEHVRARLARQICAQNTSQSG
                     YLPLIALSARWEQAFAESLIGQGEDRSLAMQPSKLSEFMTVVRDRFEQAAREGEAPVL
                     VTSAAIRPFVRSLVERFRAQTIVLSQAEIHPRARLKTVGSV"
BASE COUNT          808 a         1296 c         1393 g          799 t
ORIGIN      
        1 gccgctcgat ctcggcgtgc gggcgctcaa tacgttcgtg acctgctgcc gcggccagcg
       61 gctcggcatc ttcgccggct ccggcgtcgg caagtcggtc ttgttgtcga tgctggcgcg
      121 caatgtcgac gccgatatca ccgtgatcgg cctggtcggc gagcgcggtc gcgaggtgca
      181 ggaattcctg caggacgatc ttggcgaaga ggggctggcg cgctcggtgg tggtggtggc
      241 gacctccgat gagcccgcgt tgatgcggcg gcaggcggct tacttgactt tagcaatcgc
      301 cgaatatttt cgcgatgagg acaaggatgt gctgtgcctg atggattcgg tcacccgctt
      361 cgcgatgtcc cagcgcgaga tcggcctgtc ggccggcgag ccgccgaccg ccaagggcta
      421 tacgccgacc gtgttcaccg aattgcccag gctgcttgag cgcgccggtc ccgggatcgg
      481 cgccggcacc ataaccggca tcttcacggt actggtggac ggcgacgacc acaacgaacc
      541 gatcgcggac gcggttcggg gcattctgga tggccatgtt gtgatggagc gggggatcgc
      601 cgagcgcgga cgttttccgg cgatcaacat tctcaagtcg gtatcccgga ccatgccgag
      661 atcggccgac ccggtctatc taccggtcat cgccaagggg cgccaggtga tggcaaccta
      721 ttccgatatg gaggaattga tccggctggg agcctatcgc gccggctcga gcgccgaggt
      781 cgatgaggcg atcgccctgc gtgagccgct cgaggcgttt ctgcgccagg acaaggacga
      841 agtgaccggc ctgaccgacg gttaccggca acttgagcaa atcctcggaa gtttggaaac
      901 ggaacgctaa ctttgtcagg ccatcatccg cgccatacga gtagcgatgc cggcggggcc
      961 cttgggggag ccggttccgt cccgtttttc agaccgggac ttctggggag tatgagtcga
     1021 tgaagtcacg tgaaacgcta atccgcctga agaagtttca ggtcgacgag aagcgccgaa
     1081 gggtcgccca gatcgaaggc atgatcgccg acttccagcg tatgtcggtt gacctggagc
     1141 gggaaatcca ctccgagcag gagcgcgccg ggatcaacga cccctcgcac ttcgcctatc
     1201 cgacctatgc caaggccgca atccagcggc gcgaaaatct gacccgctcc accgacgaac
     1261 tgcgcattca gcttgaagat gccaagagcc tgctgacgga ggctttcgag gaactcaaga
     1321 aagtcgagct gctggacgag cgcgatcagg cgcgcgagcg cgccgaggaa agcgcccggg
     1381 agcaagccga cctcgatagc atcggcctga tgcgcgcacg catcggggcg attgcctgag
     1441 gcgcttcctc acctcacctg taaacatcat cgaaagcccg ggccgcgagg tccgggtttt
     1501 tcgcaagccc gcagtccacg ggccggtgat cggtggcttg gtggcacggc ttggcgcaag
     1561 gtatgatacg acacctctgg actgccgccc ctggtgcata caaaaagcga ttagcggatc
     1621 gcgatttgga gacactgaat gctgacgcca gctgagctgg tctggctgat tgccgccgtc
     1681 gcgaaagggg acgaggccgc ctttgagcgc ctttacgcgg cgacgcgggc gaaactcttt
     1741 ggcgtcgtgc tgcgtatctt gcggcgacag gatcttgccg aggaggtcat ccaggagact
     1801 tacgtcaaga tctggaacag tgccggacag ttcaatcccg gccagtcgtc gccgatcacg
     1861 tggatggcgt cgattgcccg aaaccgtgcc atcgacgtcg tgcgcaagcg gggcgaactc
     1921 tcgatcgagg aggagccggc ggcaatggaa gtggccgccg acagcccgga tccgctggcg
     1981 cgccgggaga tgagcgaaga attgaagcgc ttgctggaat gcgtcggccg tctggagccg
     2041 gaccggcaaa aactggtgct gctcgcctat tacaacggct ggagccgcga gcaattggct
     2101 gcgaagttcg agacgccggt gaacacggtg aagacctggc tgcgccgcag catgatggac
     2161 atccgggaat gccttggact ttagatgatg gcctatagcg aagacgatat cgcgctcgcc
     2221 gcggaatatg ccctcggcac tctcgatgcc gatgagcggg ccgaggccga gaccaagatg
     2281 tccgtcgaca aggacttcgc ggcgatggtt gaggcgtggc agcacaagct cggcgtcttg
     2341 aaccagatgg tcgggtcggt cgagccgcgg cccgaactct ggaacaagat caagaccgcg
     2401 attggcctgt ctgaagcgca tgctgcactc acgcttccgg cgacgccgcc ggccgcttcc
     2461 gtggccgtcg agcctgccgc cgtggcggac acctccagcg tggttcagct gtccacgcgg
     2521 gcccggcgct ggcgcaatgt tgcgaccttc gccacggcaa ttgcagctgc cctgatcgcc
     2581 atgattgcgg tcggggtcta tcagccggac ttgcttcctg gggccatccg tcccaagccg
     2641 cggacgcagg tggttgaggt caggacgcct tccgcgccga caccgcgctc cgcccagtac
     2701 gtcgccgtgc tgcagaagga gagcggggcg cctgccttca tcctcacggt ggacgcggcg
     2761 accaagagct tcacggtgcg caaggtcggc gcctcggccg aacccggcaa gagttttgag
     2821 ctctggctga tctcggacaa gctgccgcgg ccgcgctcgc tcggggtcat cggcaatagc
     2881 gacttcaccg cgcgcccggt gctggcgtca tacgatagtg acgtcatcaa caacgcgacc
     2941 tatgcggtga cgctcgagca ggcgggtgga tcgcccgacg gcgtcccgca ctcggcgccg
     3001 gtctttaccg gcaagctgat cgagaccgtt cccccggccc gttgaactcg tcagtcgaca
     3061 aaaagaaaac gcccgtgggg agcgggcgtt ttcggtagtc aacttggggg tagttggggt
     3121 cttctattat ccacgtggcg actttggggg gctggtaaag agccgcgtga attcgtgaaa
     3181 tcctttgtta gcgaacgacc gcactgttcg tgctggtgac cgtcacggcc ttgccggcct
     3241 tcatgacacg cgcagtctga gtgtagtcat cggaattggt gcgagccgaa atgccgaagg
     3301 ccgccaccgc gataccagca acgagcgcca cgaccacgat cttaaggtgg gtcgagcgat
     3361 ccgcgctgta gatcgaatgg ttcattgaag cctcctgccg ccttcccgtc tgttgcggat
     3421 ttgtctcttc cgcttgcgga caggttcaac gtctcacgat accaaacgtg gggattcggc
     3481 tttcgtttcc gaagagggga tcacaaggcc gtgaagagat gtgaagcgcc gcgatccggt
     3541 tccggggatt catctcggca agggacaaat tattcaagga ttacaattga ttgaccctag
     3601 accgcattct tggcgcaaaa tagtgccaat tgtgcccagc caagaaatcg tttgcctgtg
     3661 gcggaaatgc cctgccggtg cggcgtgcga tcagacgctt ccgaccgtct tcagccgcgc
     3721 ccgcggatgg atttccgcct gcgacaagac gatggtttgg gcccggaaac gttccaccag
     3781 cgaccgcacg aacggccgga tcgccgccga tgtcaccagc accggcgcct cgccttcgcg
     3841 cgccgcctgt tcgaaccggt cgcgcaccac ggtcatgaat tcggaaagtt ttgacggctg
     3901 catcgccagg ctgcggtcct cgccctggcc gatcagcgac tcggcaaaag cctgctccca
     3961 tctcgcggac agggcgatca acggcaggta gccgctctgg gaagtatttt gcgcgcaaat
     4021 ctgccgcgcc agccgggcac gaacatgttc gaccatcgtc gccgggttgc gcgagaacgc
     4081 cagcgcgtcg gcgatgcctt cgagaatggt cgagagatcg cggatcgaga tgcgctcggc
     4141 gagcaatagc tgcaacacgc gctggatgcc tgaaatcgta acctggctcg gcacgatgtc
     4201 cttgaccagt tcgccttgct ccttcggcag atccttcagg agtttttgca cctcgccgta
     4261 agacagcaag tccgacatgt tgttcttgag caattc
//
LOCUS       DATNOI010000123        11335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2253450,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000123 DATNOI010000000
VERSION     DATNOI010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11335
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2253450"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VN968_04535"
     CDS             <1..300
                     /locus_tag="VN968_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXL28542.1"
                     /translation="SVIKVGGITEYLKVAALADEMGVRLAPHSPYFGPGLLATLQLMS
                     LRDDGSFIEIFYTKRAACLWHGQIDADANGSIAVPEGPGLGYEPDKEIMERYRVS"
     gene            466..1788
                     /locus_tag="VN968_04540"
     CDS             466..1788
                     /locus_tag="VN968_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL28543.1"
                     /translation="MRLPFFYGWIMVAVTFVTMAIGVNARTSFSLFFPPIIDEFGWER
                     GITAGAFSFGFLASCAVSPLIGRTMDRFGPRAVMEVGVALMAGGLLLAPLTTQPWHLY
                     LTIGVMVGAGSVCLGYSGQSQFLPNWFIRRRGLAMGLAFAGVGIGSITLLPWVQHMIE
                     QTGWRTACTAMGIVVLVVLAPINLLLRKRPEDIGLRPDGDAAPSESSKPLSYVVDPDW
                     AAIDWTLKRAVRTARFWWISLGYFCGLYIWYAVQVHQTKYLLDIGFSPSVAVWALGAV
                     SLLGIPGQIWLGHLSDRVGREWIWTASCLGFAICFAALVALKYYPALLLVYLMVLTQG
                     TLGYGLTSIMGAVVLEIFQGKHYGTIFGTMMIAALAGGAAGPWVTGFLHDVSGSYTIA
                     FAIGIGVSGLSAVAIWMASPRKIRTVAGRLHRMRDAAGAPSVPSRMMK"
     gene            1982..3214
                     /locus_tag="VN968_04545"
     CDS             1982..3214
                     /locus_tag="VN968_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXL28544.1"
                     /translation="MNEHVQTATTEPLFNPLAPEFIRDPYPAYERLRTTDPMHLTPFG
                     TFAASRHAEVSLVLRDKRFGKDYVERTVRRYGPQIMEEPVFRSMSHWMLQQDPPDHTR
                     LRGLVVKAFTARRVEDMRPRIQHIVDETLDAIAGQGHMDLIEDFAFRLPVTIICDMLG
                     IPEDHREVFYKSSRDGGRLLDPVPLSPAEIAAANAGNIMAKAYFQQLFELRRRNPGDD
                     LTTQLVHAEEDGSKLSNEELTANIILLFGAGHETTVNLIGNGLLALHRNPDQLALLKA
                     NPALITNAIEEFLRYDSSVQLTGRVTLEDIDDLGGKKIPKGETVLCLLGSANRDPAVY
                     PDRPESLDIVRPNVRPLSFGGGIHFCLGAQLARIEAEVAIATLLRRLPDLRLDDAVNP
                     EWRPTFVLRGLKRLPASW"
     gene            3429..7106
                     /locus_tag="VN968_04550"
     CDS             3429..7106
                     /locus_tag="VN968_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXL28545.1"
                     /translation="MQTTLLGLAIAFIIALIAALVGPYFIDWNQFRPQFETEATRVVG
                     APVRVGGELDARLLPTPSLRLRSVTVGGANDLGKVRADKLDVEFSLGALMRGEWRATE
                     LTINGMALDLGLDSQGRIDWPASTGKFSLGTLAIDRLNLTGRIALHDASSRGTLELND
                     IAFSGDVRSLAGSVRGDGNFRLAGVRYPFRVSSGQSADASGTRVHLSIDPGERALSAD
                     LDGVLNFEARAPRFEGAVVLAAPPGAGPRGTGGEASQTPWRISARIKADPSAARLEQL
                     EASFGSEETALKITGLADIRLGGSPLLHAVLTARQLDADKFAAKDNNTAEPIRLLPGF
                     RALMVAIPSVPFPAQIEFSSEQVMLGGRPVQNLAADLHADARSWTIDRLDLRAPGATR
                     VSLNGTSAKVGPSSDFTGALSVDSADPDALVAWLQGRSEVTHRSQKPLRLSGDVSVTS
                     SSIAIDAMKAEIDGGAVEGRLAASNQATGGGSRFYADLKAERLDLDAAAAFVRPLAGS
                     PTEWPDEAQVSLAIGRATSAGQELRPFMTKLGYGPKSISLDQLKIGEPHGVMMEGAGS
                     FDRVNGTGRLALNSSAASLGQITGLIAPLAPALASRLGAMEQGPGPARLKLALDLDKN
                     AEHADRADARAVFDLDAPQLKGVVTITATPAIAAVRGIDLDALQHSEVGIESKLTSAR
                     GGSLLVLLGLDRTIAVGEGPLEFQGSATGIWHAPLLLHVKITGAGLDAEAQGIAEPWA
                     QEPKAGVSLKVRSINLAPLFDLKPSDALAQNISLSSHVSLAGSRLILDDIDTAMSGSR
                     LRGRLALTLDEPRTVAGEVGLDVLDLAPAFALAIGAAGHDAAEPLGAGLVKGWRGRIA
                     FQALRGSLPGGGELRPVSGTVKSDGQSLTFEAINGGLGGGEATANLDAKQSANGIALN
                     ARVQLSSVDGAVLRYRGLAMPAGRASMQMTLASQGRSASALAGALSGSGTLALESARV
                     AGLDPRAFDAAIRASDNGQATNDVMLRRIVEPALSAGALSVASAQIPFNIRDGRIRVG
                     ATTLDAEGARAIVSGGYDIPADQADIRVTIASTTAGAAASRPEIQLLSVGSPDALIRT
                     VDVAALSSWLAVRAIDRETRRLDSIERGDPTAALPASIAPSAPAQPSTGASDADLPGL
                     PLSEVPASGRDPRRPPSRPRIGVPHLPLAPSNSPVAAQQVAPLPPPIEVRPPPSIMRP
                     PKPRPPLALTPPAAAPPRPTF"
     gene            7288..8016
                     /locus_tag="VN968_04555"
     CDS             7288..8016
                     /locus_tag="VN968_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HXL28546.1"
                     /translation="MTKRILLGMLTPSSNTALEPITTAMVAGLPEVSAHFSRFKVTEI
                     ALSNTALAQFDNDEILRAAELLAHAKVDSIGWNGTSSGWLGFEADVRLCEQITAATGI
                     QATTSMLALNEILERKKVTRLGYVTPYLDEVQARILENYGKLGIACQGERHLGLQDNF
                     SFSEVPVAQLENMTREVAQDGPDAITIICTNLRVAPSVAKLEREIGIPIYDTIATVVW
                     KCLKLAGVDTKRVVDWGSLFQQVA"
     gene            complement(8197..8457)
                     /locus_tag="VN968_04560"
     CDS             complement(8197..8457)
                     /locus_tag="VN968_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HXL28547.1"
                     /translation="MKSPVVKRSIVVAGHKTSVSLEEAFWNGMKEISGLRGMTLSELV
                     GEIDSNRQQGNLSSAIRLFVLDYFRTRAVSAAPEPKSAQVSH"
     gene            complement(8454..8642)
                     /locus_tag="VN968_04565"
     CDS             complement(8454..8642)
                     /locus_tag="VN968_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HXL28548.1"
                     /translation="MGDVVNLKRFKKRAEKQQSAKQAEANRTRFGRTKSERTLDQQRT
                     SRASDLLDQHRIDGEDAS"
     gene            complement(9031..10449)
                     /gene="fumC"
                     /locus_tag="VN968_04570"
     CDS             complement(9031..10449)
                     /gene="fumC"
                     /locus_tag="VN968_04570"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HXL28549.1"
                     /translation="MDRPATSPRSNSTRSETDSFGPIEVSADRYWGAQTERSRRNFRI
                     GQDRMPMPIVHALGIVKLAAAETNRELALLDQRRARAIVCAAREVIDGKLDDHFPLVV
                     WQTGSGTQTNMNLNEVIANRANELLGGELGAKTPVHPNDHVNMSQSSNDSFPTAMHIA
                     AAMGIVGDLIPALRELHRALRKKEKAFAHIVKIGRTHTQDATPLTLGQEFSGYAAQVE
                     SGIARLRVAVKDLYPLAQGGTAVGTGLNSKPKFAKAFARHVAKITKLRFTSAPNKFEA
                     LASNDAYVFAHGAINSVATGLFKIANDIRLLGSGPRSGLGELILPENEPGSSIMPGKV
                     NPTQCEASTMVCCQVFGNHTTITIAGSQGHFELNVYKPVLAYCMMHSIQLMSDVARSF
                     TEHCVEGIRADEKRIRELMERSLMLVTALAPRIGYDNAAKVAKSAHARGTTLKEEAVR
                     LGFVSAAEFDRLVQPVKMIHPG"
     gene            complement(10548..11069)
                     /locus_tag="VN968_04575"
     CDS             complement(10548..11069)
                     /locus_tag="VN968_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HXL28550.1"
                     /translation="MATDHIRYDVLARDALRGVLRRVLTDAAEHGLPGEHHFFITFLS
                     TAEGVKLSPRLLAQYPEEMTVILQHQFWDLTVTEDRFEVGLSFGGIAERLVVPFNSIK
                     SFFDPSVQFGLQFEPSDAAADAPVASLPAVAAPSSTASALTVPAPPPENKDEPAKPGE
                     GAEVVRLDRFRKK"
BASE COUNT         1987 a         3511 c         3625 g         2212 t
ORIGIN      
        1 tcggtgatca aggtcggcgg catcacggaa tatctgaaag tcgccgcatt ggccgatgaa
       61 atgggcgtca ggcttgcacc gcattcgccg tatttcgggc caggccttct ggcgacgctg
      121 caactgatgt cgctgcgtga cgacggctct ttcatcgaga ttttctacac gaagcgcgcc
      181 gcctgtcttt ggcatggcca aatcgatgcc gatgcaaatg gcagcatcgc ggtgcccgaa
      241 gggccggggc tgggctatga gccggacaag gagatcatgg agcgttaccg ggtgtcatga
      301 ccgatatgcc cagacggcgc ttagccattc cgcgacatct tgaagacatc caggaagaga
      361 tgacttgacc tggtcattcc gggatggtcc gaaggaccag acccggaatt tcgagattcc
      421 gggttcgcgc tgttcgcccc ccggaatgac gagatgcgaa accccatgcg gcttcccttc
      481 ttttacggct ggatcatggt cgccgtgacg ttcgtcacca tggcgatcgg cgtcaacgcg
      541 cgcacgtcgt tttcgttgtt ctttccaccc atcatcgatg agttcggctg ggagcgcggc
      601 atcacggcgg gcgccttctc gttcggtttc ctggcttcct gtgcggtcag tcccttgatc
      661 ggccgcacga tggatcgctt tggcccgcgc gccgtgatgg aggtcggcgt cgcgctgatg
      721 gcgggaggct tgttgctggc gccgctgacg acgcagccat ggcatctgta tttgacgatc
      781 ggcgtcatgg tcggcgccgg cagcgtctgt ctcggttact ccggtcaatc gcagtttctg
      841 cccaactggt tcattcgtcg ccgcggcctt gcgatgggac tggccttcgc cggcgttgga
      901 attggttcca ttacgttgct gccgtgggtg cagcatatga tcgagcaaac cggctggcga
      961 accgcctgca cggcgatggg tattgtggtt ctggtcgtgc ttgcgccgat caacctgttg
     1021 ctgcgcaagc ggccggaaga catcgggctt cggcctgatg gagacgcagc accttcggag
     1081 tcgtcgaagc cgctctcgta tgtcgtcgat cccgattggg ccgccatcga ctggaccttg
     1141 aagcgcgccg ttcgcaccgc acgcttctgg tggatttcgc tcggctattt ctgcggattg
     1201 tatatctggt acgcggtgca ggttcaccag accaaatatc tcctcgacat cggcttcagc
     1261 ccgagcgtcg cggtgtgggc tttgggcgcg gtcagtctgc tcggcattcc cggtcagata
     1321 tggctcggac acctgtccga tcgggtcggg cgggaatgga tctggaccgc gagctgcctc
     1381 ggatttgcga tctgctttgc ggcactggtc gccctgaaat actatccggc gttgctgctg
     1441 gtctacctca tggtgctgac gcaaggcacg ctcggctacg gtctgacgtc catcatgggc
     1501 gcggtggtcc tggaaatatt tcagggcaaa cattacggta ccatttttgg taccatgatg
     1561 atcgcagccc tcgccggagg ggcggcgggc ccctgggtga cgggctttct gcacgacgtc
     1621 tcgggaagct atacgatcgc gtttgcgatc ggtatcggcg tgagcggatt gtcggcggtg
     1681 gcgatctgga tggcgtcacc tcgcaagatc agaaccgtgg cgggccggct gcaccggatg
     1741 cgggacgctg ccggcgcgcc ctctgtcccg tcacggatga tgaaatagtc cgaagcggag
     1801 ggtggcggcg ccgacgcaag tgccggcggc gatggccccc ggatgtcctg ttctcgctcg
     1861 aggttcgacc gggcgccaat ggagtacaac cctgagaaga gttgatcatg cggggcttgc
     1921 cgataccggc ggcttgtggg ctaatccggc tccagattga tctgaccgca tcgaggcgcc
     1981 catgaacgag catgttcaga ccgcgacgac cgagccgctg ttcaacccgc tggcgccgga
     2041 gttcattcgc gatccctatc cggcctacga gcggctgcgc accaccgatc cgatgcatct
     2101 cacgccgttt ggtactttcg cggcgagccg gcacgccgag gtcagtctcg tgctgcgcga
     2161 caagcgcttc ggcaaggatt atgtcgaacg caccgtgcgc cgctatggac cgcagatcat
     2221 ggaggagccg gtcttccgca gcatgagcca ctggatgctg cagcaggacc cgcccgacca
     2281 cacccggctg cgcggtctgg tggtgaaagc gttcacggcg cgccgggtcg aggacatgcg
     2341 tccgcgcatc cagcacattg tcgatgaaac gctcgatgcc atcgccgggc agggccacat
     2401 ggacctgatc gaggattttg cgtttcggct gccggtgacg atcatctgcg acatgctcgg
     2461 aattcccgag gaccaccgcg aggttttcta caagagctcg cgcgatggcg gacgcctgct
     2521 cgatccggtg ccgctgtcgc cggccgaaat cgccgcagcc aatgccggca acatcatggc
     2581 caaggcctat ttccagcagc tgttcgagct caggcggcgc aatcccggtg acgacctgac
     2641 gacgcaattg gtgcacgccg aggaagacgg cagcaagctc tccaatgagg aactcaccgc
     2701 caatatcatt ctgctgttcg gcgccgggca cgagaccacc gtcaatctga tcggtaacgg
     2761 tctgctggcg ttgcatcgca atcccgatca gctcgcgctg ctcaaggcca atcccgccct
     2821 catcaccaac gccatcgaag agttcctgcg ctatgactcc tcggtgcaac tgaccggccg
     2881 ggtcacgctg gaagacatcg acgacctcgg cggcaagaaa atcccgaagg gtgaaaccgt
     2941 gctctgcctc ttgggttcgg ccaaccgcga cccggcggtc tatcccgacc ggccggaaag
     3001 tctcgatatc gtcagaccca acgttcgtcc gctgtcgttt ggcgggggca tccatttttg
     3061 cctcggcgcc cagttggcgc gtatcgaggc agaggtcgcg atcgcgaccc tgttgcgtcg
     3121 cctgccggat ctgcggctgg atgacgccgt taaccctgaa tggcggccga cctttgtctt
     3181 gcgcggcctc aagcggctgc ccgcgagctg gtgacgacgc ctctcgcgtg aagccgtcac
     3241 acgaggttgt tgtgacttcg gcacagatga ctctatatag agcattgttc cggcgctgat
     3301 tagcagagcg cggcggcggc ccggcggctt cgggctaccg tcgcgatggc aggtccgacc
     3361 tggcggatga atcgcgtcat gcgccgcgcg aggccagcgc gccggaacct gctcaaagag
     3421 gagacaccgt gcagacgacg ctgctcggac tggcgatcgc ctttatcatc gcgctgatcg
     3481 ccgcgctggt cgggccgtat ttcatcgact ggaatcagtt ccggccgcaa ttcgagacgg
     3541 aggccacgcg ggtggtcggc gcgccggtgc gcgtcggcgg cgaactggat gcgcgcctgt
     3601 tgccgacgcc atcgctgcgg ctgcgctcgg tgaccgtcgg cggcgccaac gacctcggca
     3661 aggttcgcgc cgacaagctg gacgtcgagt tcagcctggg cgcgctgatg cgcggcgaat
     3721 ggcgcgccac cgaactgacg atcaacggca tggcgctcga tctcggcctc gattcgcaag
     3781 gccggatcga ttggccggca tcgaccggaa aattcagtct cgggacgctt gccatcgacc
     3841 gcttgaacct gaccggccgt atcgcgctgc atgacgcctc aagccgcggc acgctcgagc
     3901 tcaacgatat cgccttcagc ggagacgtgc gctcgctggc cggatcggtt cggggcgacg
     3961 gcaacttcag gctggccggg gtgcgttacc cgtttcgggt gtcatccggt cagagcgccg
     4021 acgccagcgg cacccgcgtc catctcagca tcgatcccgg cgagcgcgca ttgtccgccg
     4081 atctcgacgg cgtgctcaac ttcgaggcgc gtgcaccgcg cttcgaaggc gcggtggtgc
     4141 tggccgctcc ccccggagcc ggaccgagag gcactggcgg cgaggcgtcg cagacgccgt
     4201 ggcggatctc ggccaggatc aaggccgatc cttcagccgc gcggctcgaa cagctcgagg
     4261 ccagctttgg ctccgaagag acggcactga aaatcaccgg gcttgcggac attcggttag
     4321 gaggatcgcc gctgctgcat gccgtgctca ccgcgcggca gctcgacgcc gacaagttcg
     4381 ccgccaagga caacaacacc gccgagccga ttcgactgtt gccgggattc cgcgccctga
     4441 tggtggcgat tccttcggtt cctttcccgg cgcaaatcga attcagctcc gaacaggtca
     4501 tgctgggcgg ccgcccggtg cagaatcttg cggccgatct gcatgccgac gccagatcgt
     4561 ggaccatcga ccggctggat cttcgcgccc cgggcgcgac ccgcgtgagc ttgaacggca
     4621 ccagcgcgaa agtcggcccg tccagcgatt tcaccggggc gctcagcgtc gactccgcgg
     4681 atccggacgc gctggtggcg tggttgcagg gccgcagtga ggtcacgcat cgcagccaga
     4741 agccattgcg cctgagcggc gatgtcagcg tgacctcgag cagcattgcc atcgacgcca
     4801 tgaaggcgga gatcgatggc ggcgccgttg aagggcggct ggcggcctca aatcaggcga
     4861 cgggtggcgg ctcgcggttc tacgcggatt tgaaggcgga gcgtctcgat ctcgatgccg
     4921 ctgcggcttt cgtgcgtccg ctggccggct cgccaaccga gtggcctgac gaagcgcagg
     4981 tatcgctggc tatcggccgc gccacttcgg ctggtcagga attgcgaccc ttcatgacca
     5041 agctcggcta cggtccgaaa agcatctcgc tggatcaatt gaaaatcggc gaacctcatg
     5101 gcgtgatgat ggaaggtgcg gggagtttcg accgcgtcaa tggcaccgga cgattggcgc
     5161 tgaattcaag cgccgcgtcg cttggtcaaa tcaccgggct gatcgcgccg ctcgcgccgg
     5221 cactggcgtc gcggctcggc gcgatggaac agggcccggg tcccgcgcga cttaaactgg
     5281 cgcttgatct cgacaagaac gccgaacatg ccgatcgcgc cgacgcgcgc gccgttttcg
     5341 atctcgacgc gccgcagctc aagggggtcg tcacgatcac ggcaacgccc gcgatcgcgg
     5401 ccgtgcgcgg catcgatctc gatgcgctcc agcacagcga agtcggcatt gaatcgaaac
     5461 tgacctcggc gcgcggcggt tcattacttg tcttgctggg gctcgatcgc accatcgccg
     5521 tgggcgaagg cccgctggaa tttcaggggt cggcgaccgg catctggcat gcgccgttgc
     5581 tgctgcacgt gaagatcacg ggagctggcc tcgatgccga agcgcaaggt atcgccgaac
     5641 catgggcgca ggagcccaag gccggcgtca gtctgaaagt tcgcagcatc aatctcgcgc
     5701 cgctgttcga tctcaaaccc tccgacgcgc tggcgcaaaa catcagcctt tcctcgcacg
     5761 tttcgctggc gggcagcagg ctgatcctgg acgacatcga caccgcgatg tcgggttcgc
     5821 gactgcgcgg gcgtctggcc ttgacccttg acgagccaag gaccgtggcg ggcgaagtcg
     5881 gcctcgatgt gctcgatctg gcgccggcat ttgcgctcgc catcggcgcc gcggggcatg
     5941 atgcggccga gccgttggga gccggactgg tgaaaggctg gcgcggtcgt atcgcgttcc
     6001 aggcgttgcg cggctcgctt ccgggcggcg gcgagttgcg ccccgtgagc ggtaccgtca
     6061 aaagcgacgg ccaatcgctg accttcgagg cgatcaacgg tggactgggc ggcggcgagg
     6121 cgaccgccaa tctcgacgcg aagcagtccg cgaatggaat tgcgttgaac gcgcgcgttc
     6181 aactcagcag cgtcgacggt gcggtcttgc gctatcgcgg cctcgcgatg cccgcgggcc
     6241 gcgcgtccat gcagatgacg ttggcgagcc agggccgcag cgcgtcggcg ctggcaggcg
     6301 cgctgtccgg aagtgggacg ctggcgctgg aatccgctcg cgtcgccggg cttgatccgc
     6361 gtgcgttcga cgccgcgatc cgcgccagcg acaacgggca ggcgacgaac gacgtcatgt
     6421 tgcggcggat cgtcgagccg gcattgtcgg cgggcgcgtt gtcggtggca tcggcgcaaa
     6481 ttccattcaa catcagggat ggccggattc gtgtcggcgc gaccacgctc gatgccgagg
     6541 gcgcccgcgc catcgtatcg gggggatacg atattcccgc cgaccaggcc gatattcgcg
     6601 tcaccatcgc ttcgacgacg gccggagccg ccgccagtcg tccggaaatt cagctgttgt
     6661 ctgtcggctc gcccgacgcg ctcatccgca cggtcgacgt cgcggcgcta tcgtcatggc
     6721 tggcggtgag ggcgatcgac cgcgaaaccc gccggctgga ttcgattgag cgcggcgatc
     6781 cgacagcggc gttgccggcg tcgattgcac cgtccgcgcc cgcgcaacct tcgaccggag
     6841 cgtcggacgc ggacctgccc ggcctcccgc tttccgaggt ccccgcttcc ggtcgcgatc
     6901 cgcggcggcc gccgtcgagg cccaggatcg gcgttccgca tttgccgctt gctccctcaa
     6961 attcgccggt tgccgcccag caggtcgcgc cactgccgcc gccgatcgag gttcgcccgc
     7021 cgccaagtat catgcggccg ccgaagccgc ggccaccgct ggcgctgacg ccgccggctg
     7081 cagcgccacc gcggccgacg ttctaagcca aagcgcggcg ccatgcttca tagccagaat
     7141 gcgcgcgagc ttgatcggcc tgcacgcgat cgtctgcgcc cgaaccgacg ttgaaaatca
     7201 ggcgttgctc tcacccacca aacgacacat tctcgatttg ctgtgctcgg agcgggcgaa
     7261 ttcaatcctg gatgccatgg gtcaccaatg acaaagcgta ttcttcttgg aatgttgacg
     7321 ccgtcgtcga atacggcgct ggagccgatc acgaccgcga tggttgccgg gctgccggaa
     7381 gtgtccgcgc acttttcgcg cttcaaggta accgaaatcg ccctgtccaa tacggcgctg
     7441 gcgcaattcg acaatgacga gatcctgcgt gccgcggaat tattggcgca tgccaaggtc
     7501 gactcgattg gatggaacgg cacatcctcc ggctggctgg gctttgaggc cgatgttcga
     7561 ttgtgcgaac aaatcaccgc cgcaaccggc atacaagcga cgacgtcgat gctggccctc
     7621 aacgaaatcc ttgaacggaa gaaagtgacg cggctcggct acgtaacgcc ctatctcgat
     7681 gaagtgcagg caagaatcct ggaaaattat ggaaagctcg gcattgcctg ccagggtgag
     7741 cggcaccttg gcctgcaaga taatttttcc ttttccgaag tgccggtcgc gcagctggaa
     7801 aacatgaccc gcgaggtggc gcaggacgga ccggatgcga tcacgatcat ctgcaccaat
     7861 ttgagggtcg caccatcggt tgcgaagctg gagcgcgaga tcggcattcc catctatgac
     7921 accattgcca ccgtggtctg gaaatgcctc aagcttgccg gcgtcgatac caagcgcgtc
     7981 gtcgattggg gttcgctgtt tcaacaggtc gcatgattgc atccgactga tccgaattct
     8041 tgccggagat atcgaccatg gctgcgttcg acacggtcat tcgtcacggt gcgattgcca
     8101 ccgttcgcgc ctttgtcgat gccgccgcat cgataagcgg tcgttgcggg ccgtcccctc
     8161 gatcaaattc gaggacgcgg tatctcggaa tacgcgttag tgagacacct gcgccgattt
     8221 tggttccggc gcggcggaca ccgcgcgggt tcgaaaataa tcaaggacga agaggcggat
     8281 ggccgaagac agattgcctt gctggcggtt gctgtcgatc tcacccacca gttcggacag
     8341 tgtcatgcct cgaaggcccg agatctcctt catgccgttc cagaatgcct cttcaaggct
     8401 gacgctggtc ttgtgacccg cgaccacgat cgagcgtttc accacaggcg atttcatgac
     8461 gcgtcctcgc cgtcgatccg atgttgatcc agtaaatcgc ttgcccgact tgtacgctgt
     8521 tggtccagcg tgcgttcgga tttggttcgg ccaaaccgtg tccggttggc ctccgcctgt
     8581 ttcgccgatt gttgtttctc ggcgcgcttc ttaaatcgct tcaggttgac cacgtccccc
     8641 atgatcgccc ccgtcatgaa ataagctgat gggatacggc atcttcacga agccgcggga
     8701 ggccgcgctg ggcatgagag caagttccgg ctaagcgcaa tccggtcagc gtcaggaaaa
     8761 tgttctcgat caataccctt gggcgtatcg cagttgcaaa accgctattc gcacttgcga
     8821 aatacgcgcc agccggctca gtcatcattc ggaaaatctc ctaccttgag tagcgacaaa
     8881 aaaatgctcc gcttttgtga agacgaaata ttttcgtgct ctagcacggg cctgcgcgac
     8941 ggcgatagct taacggtatt attctttagg cgttatatga ctcgcaaata attttcgacc
     9001 ccgtagttct acgggaatcg cgccgcggca tcagcccggg tgtatcattt tcaccggctg
     9061 caccagccga tcgaattctg ccgccgagac aaagcccagc cgcacagcct cttccttcaa
     9121 cgtagtgccg cgggcgtgag ctgacttcgc aaccttggcg gcattgtcgt agccgatcct
     9181 gggagcgagc gcggtcacca gcatcagcga gcgttccatc aattcccgga tacgtttttc
     9241 gtcggcgcgt attccctcga cacaatgctc cgtgaacgag cgtgcgacgt cggacatcaa
     9301 ttggatggaa tgcatcatgc aataggcgag caccggcttg tagacgttca gttcgaaatg
     9361 tccctggctg cccgcaatgg tgatagtcgt atggttgccg aacacctgac agcaaaccat
     9421 ggtcgacgct tcgcattgtg tcgggttgac cttgcccggc atgatcgacg agccgggttc
     9481 gttttcaggc aggatcaatt cgccgaggcc ggaacgcgga cccgatccca acaggcggat
     9541 atcgttcgcg atcttgaaca ggccggtcgc caccgaattg atcgcgccat gcgcgaatac
     9601 gtaggcgtcg tttgaggcca gcgcctcgaa tttgttggga gcgctggtga agcgaagctt
     9661 tgtgattttg gcaacgtgtc tggcgaacgc cttggcgaat ttcggcttcg aattgagtcc
     9721 ggtaccgaca gcggtgccgc cctgcgccag tggatacaga tccttgaccg cgacgcgcag
     9781 ccgcgcgatg ccgctctcga cctgggcggc gtagccggag aactcctgac cgagcgtcag
     9841 cggcgtggcg tcctgggtat gggtgcggcc gatcttgacg atgtgagcga acgccttttc
     9901 ctttttgcgc agcgcgcggt gaagctcgcg gagcgccgga atgagatcgc cgacgatgcc
     9961 catcgccgcg gcgatgtgca tcgcggtcgg aaatgagtcg ttcgacgact gactcatatt
    10021 gacgtgatcg ttgggatgaa ccggtgtctt ggcgccgagc tcgccgccca gcagttcgtt
    10081 ggcgcgattg gcgatcacct cgttgaggtt catgttggtt tgcgtgccgg agccggtctg
    10141 ccagaccacc agcggaaaat gatcgtcgag cttgccgtcg atcacctcgc gcgcggcgca
    10201 gacgatggca cgggcgcggc gctggtcgag caaggcgagt tcgcgattgg tttccgccgc
    10261 cgcgagcttg acgatgccga gcgcatgaac gatcggcatc ggcatccggt cctggccgat
    10321 acggaaattc cgccgcgacc gttccgtctg cgcgccccag tagcgatcgg cggaaacctc
    10381 gatcggaccg aagctgtcgg tctcggagcg cgtcgaattg gatcgcgggg atgttgctgg
    10441 tcgatccatg aatcatgtcc gttacgcaaa tacgactatg tcggaaccag ctcacgccag
    10501 ctccgcggac gttcattcta cagcggcgcg gatctctcgc gcgcggatta tttcttgcgg
    10561 aagcgatcca gccgcacgac ttcggcgccc tcgcccggct ttgccggttc gtccttgttc
    10621 tcggggggcg gcgcgggaac cgttagcgcc gaagctgtgg aagagggcgc agcaaccgcc
    10681 ggaaggcttg ccaccggcgc gtcggccgcg gcatcggacg gttcgaactg gaggccgaat
    10741 tgtaccgacg ggtcgaagaa gctcttgatg gaattgaacg ggaccaccag gcgctcggcg
    10801 atgccgccga acgagaggcc gacttcaaac cgatcttcgg tcactgtcag atcccagaac
    10861 tggtgctgga ggatgaccgt catttcttcc ggatattgcg ccagcagccg cggcgacagc
    10921 ttcacgcctt ccgcggtcga cagaaaggtg atgaagaaat gatgctcgcc aggcaggcca
    10981 tgctcggcgg cgtcggtcag aacgcggcgc agcacgcccc gcagcgcatc gcgcgccagg
    11041 acgtcgtatc ggatatgatc ggtcgccatt gtcggtcctg tcacgttcgg atgccgccgg
    11101 cggatcggca acccgactcg cccctttcgt acatgctact ccgaagccgg cctgaggtca
    11161 gcaggtgttg ccgccggcgg tgcaaattcc agcaatctgg gctggaatat gatttgggat
    11221 gaggcaaatt caaatcgaag tggaggcttc tgttgccagg tgcctccgaa ccccgcctga
    11281 cggagcttaa cccgtcagga cttcagactt ggtttttcaa accgcttacg cggcc
//
LOCUS       DATNOI010000124         3966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_496894,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000124 DATNOI010000000
VERSION     DATNOI010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496894"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VN968_04580"
     CDS             <1..418
                     /locus_tag="VN968_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL28551.1"
                     /translation="GAPAINDLIPGRVDVIFDNMPTVLEHVKAGSLRGLAVTTKERVA
                     VVPDLPTISESGLPGFDVFSWFGFFVPARTPEGVIARINADTNAALVYPSVKSRFEDL
                     GATPKGSTPVELAAFLKSEIDKWGPVIRDAKIRVEN"
     gene            665..740
                     /locus_tag="VN968_04585"
     tRNA            665..740
                     /locus_tag="VN968_04585"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Phe,seq:gaa)
     gene            complement(798..1811)
                     /locus_tag="VN968_04590"
     CDS             complement(798..1811)
                     /locus_tag="VN968_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXL28552.1"
                     /translation="MQYRNLGRSGLKVSPICLGTMMFGGPTDEATSARIIAKAREAGV
                     NFIDTADAYNNGNSESVVGRAIANNRQNWILATKLANQVGDDPNRGGLSRRWVLQAAD
                     ESLKRLGTDFIDIYYLHKEDHSTPLEETVRAMGELMRQGKVRYFGVSNYRAWRLAEIC
                     NICDNIGIDRPVASQPCYNALNRMPEVEHFPACSYYGLGIVPYSPLARGVLTGKYRPD
                     AAPDKDTRAGRNDKRMMQTEWRPESILLAQEIKRHAEAKGITSGQFAVSWVLNSSFVS
                     SVIAGPRTEAQWDDYLRALDYRFTAEDEALVDRLVASGHPSTPGFNDPAYPIEGRRAR
                     TAG"
     gene            complement(2050..2472)
                     /locus_tag="VN968_04595"
                     /pseudo
     CDS             complement(2050..2472)
                     /locus_tag="VN968_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030078.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
     gene            2540..3172
                     /locus_tag="VN968_04600"
     CDS             2540..3172
                     /locus_tag="VN968_04600"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HXL28553.1"
                     /translation="MIWPFDEATRQNIAARCAAFARLPASEPAPGLKRAAVAIALVEP
                     GGVGRGTALLLTLRAAGLRSHRGQWALPGGRCDEGESPMMAALRELHEELGLELTRGA
                     VLGLLDDYPTRSGYLITPVVVWAKAGASISPNPQEVASVHRIALDTIAQADAFDFTAI
                     PESTRRVIRFRHAGRFIHAPTAALIYQFREVLQGRDTRVTELEQPVFAWK"
     gene            3400..>3966
                     /locus_tag="VN968_04605"
     CDS             3400..>3966
                     /locus_tag="VN968_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL28554.1"
                     /translation="MRIWKIWLCAAAMLFGLPVASVDAQAQDFPNRAITLVIPFAPGG
                     STSIVGRVIADKMSELLGEKVVVDNRPGAGGTVGTKAVAKSDPDGYTLLLGYTGTLAI
                     GPSLYKNAGYDPRKDFAPIGLIGNAPNSLVVHPSFPARTVTELIAYAKANPGKVNFGS
                     AGAGTASHITGEYFARSAGITLVHIPDKG"
BASE COUNT          687 a         1253 c         1243 g          783 t
ORIGIN      
        1 cggggcgccc gcgatcaacg acctgattcc cggccgcgtc gacgtcatct tcgacaacat
       61 gccgacagtg ctggagcatg tgaaggccgg cagcctgcgc gggctggcgg tgacgaccaa
      121 agagcgcgtc gccgtcgtgc ccgacctgcc gaccatttcg gagtctggct tgcccggctt
      181 cgacgtgttt tcgtggttcg gcttcttcgt gcccgcacgg acgcccgagg gcgtcattgc
      241 caggatcaac gccgacacca atgcggcgct ggtctatcca tcggtcaagt cgcggttcga
      301 ggacctcggc gcgaccccga aaggctcgac cccggtcgag ctcgccgcct tcctgaaatc
      361 ggaaatcgac aaatggggcc cggtcatccg cgacgcgaag atcagggtcg aaaactgagc
      421 ccagagttgc caggacctca ctaacggcat tctcactatc ccgctcacgg cttggaaaat
      481 gagtcgtttt ccgtcatggc cgggcttcgt cccggccatc cacgtctttc ccttccggga
      541 agaggacgag aacgcggaat gaccgtcatt tcgtgcgttt ttcggcccaa cgccgggcgg
      601 gtagacagag caagcgagcc tgactataaa ccgcccgctc cccgggcctc gcgcccttgg
      661 aagtgcccag gtagctcagt tggtagagca tgcgactgaa aatcgcagtg tcggtggttc
      721 gattccgccc ctgggcacca ttccggcaga tcagacttag ctcggcgatt ttattgacgc
      781 cgaatgaccc tgacattcta ccccgccgtc ctcgcccgcc gtccctcgat cggatacgcc
      841 ggatcgttga accccggcgt cgagggatgg ccgctggcca ccaagcggtc caccagcgct
      901 tcgtcctcgg cggtgaagcg atagtcgagc gcgcgcagat aatcgtccca ttgcgcttcg
      961 gtgcgcgggc cggcgatcac gctcgatacg aacgaagagt ttagcaccca tgacaccgcg
     1021 aactggcctg atgtgatgcc tttggcttcg gcgtgccgtt tgatctcctg cgccagaaga
     1081 atcgattccg gccgccactc ggtctgcatc atgcgcttgt cgttgcggcc tgcgcgggtg
     1141 tccttgtcgg gcgcggcatc ggggcggtat ttgccggtca gcacgccgcg cgccagcggg
     1201 ctatagggca cgatgccgag gccgtaatag ctgcaggccg ggaaatgctc gacctcgggc
     1261 atccggttca gcgcgttgta acagggctgg ctcgcgaccg ggcgatcgat gccaatgtta
     1321 tcgcagatgt tgcagatttc ggcgaggcgc caggcgcgat agttcgagac gccgaaatag
     1381 cgcaccttgc cctgccgcat cagttcgccc atcgcgcgca cggtttcttc cagcggcgtc
     1441 gaatggtctt ccttgtgcag gtaatagatg tcgataaagt cggtgccgag ccgcttcagg
     1501 ctttcgtccg ccgcctgcag cacccagcgc cgcgacagcc cgccgcggtt gggatcgtcg
     1561 ccgacctggt tggcgagctt tgtcgcgagg atccagttct gccggttgtt ggcaatggcg
     1621 cggccgacga cgctttccga attgccgtta ttataggcgt ccgcggtgtc gatgaagttg
     1681 acgccggcct cgcgcgcttt ggcaatgatg cgcgccgacg tcgcctcatc ggtcggcccg
     1741 ccgaacatca tggtgcccag gcagatcggc gagactttca ggccgctgcg gccgaggttg
     1801 cggtattgca tggggtgggt cctttacaaa aatggctcga cgttagctcc ctcgccccgt
     1861 tcttcacggg gagagggttg gggtgagggg ctttctccgc gaatgctgaa acataccatg
     1921 cgcggcgacg tctccacaga tcgtcattgc gagcgcaagc gaagcaatcc atctcaccac
     1981 gcgaagaaag aatggattgc ttcgtcgctt cgctcctcgc aatgacgggg agggcagcgc
     2041 cgtcgcccct cactcgctct tcaatatctt gaacacgcct gaggccatgg cgatacagcg
     2101 cttgtcgacg gtcacctcgg tcgtgatgaa gatcaggctg cgggtcgagc gcaccacgtg
     2161 cggcttcgag accaggatct cgccgatctt gccaggctcg acgaaatgcg tatcgagctg
     2221 cacggtcgcc agcatcggct ttcccgagac gtatcacgcc gtcatgccgc aggtgcggtc
     2281 cgcgaacgtc atcagtaccc ctccctgcac caggccgcgg cggttgtggt gcttgtcctg
     2341 cgctacaagc gcatattcgt gctcgccgtc gacgacccgt tgccacagcg gcccgaccag
     2401 gctgatgaac ccgctggttt caacttttgt ccagccggcg gccttgagct tcgccgtggc
     2461 cttgtcggtc attttgtttt gttctccgcg gtcggcgtca gtcatttcat caagcgcgga
     2521 ggtgttgtag tcaagcggga tgatctggcc attcgacgaa gccacccgcc aaaatatcgc
     2581 ggcgcgctgc gcggcgtttg cgcggctgcc tgcgagcgaa ccggcgcccg ggctgaagcg
     2641 ggccgcggtc gccatcgcgc tggtcgagcc gggaggtgtg ggacgcggaa cggcgctgct
     2701 attgacgctg cgcgccgcgg ggctgcgctc ccaccgcggt caatgggcgt tgccgggcgg
     2761 acgctgcgac gagggcgagt cgcccatgat ggcggcgctg cgggagcttc acgaagagct
     2821 cggccttgaa ctcacgcgcg gcgctgtgct gggcctgctc gacgattatc cgacgcgctc
     2881 cggctatctg atcacgcccg tcgtggtgtg ggcgaaggcg ggcgcgagca tctcgcccaa
     2941 tccgcaagag gtcgcctcgg tgcatcgcat cgcgctcgat actatcgcac aggctgatgc
     3001 cttcgatttc accgccattc cagagagtac gcggcgcgtg atccggtttc gtcacgccgg
     3061 aagattcatc cacgcgccga ccgcggccct gatctaccag ttccgcgaag tgctgcaggg
     3121 gcgcgacacc cgcgtgactg agctggaaca gccggtgttc gcctggaagt aggccatgaa
     3181 ttaagacggt cattgcgagc gcagcgaagc aatccatcgt gccacaaaaa gaaagaatgg
     3241 attgcttcgt cgcggagcct gtcatcgggc gcgcgttcgc gcgacccgtt ggctcctcgc
     3301 aatgacgaga aaatgtattg ttgtccggca gtcgggttgc gaacatcgca gctttcgacg
     3361 accataggca aaacaataat gccgggaggc cctgcaaaaa tgcgtatctg gaagatctgg
     3421 ctttgtgccg ccgcaatgct gttcggcttg cccgtcgcat cggttgatgc gcaggcgcaa
     3481 gactttccga accgggccat cacgctggtg attccgttcg cgcccggcgg ctctacctcg
     3541 atcgtcggcc gcgtgatcgc cgacaagatg agcgaattgc ttggcgagaa agtcgtggtc
     3601 gataaccggc ccggcgccgg cggcaccgtc ggcaccaagg cggtcgcgaa aagcgatccc
     3661 gatggctaca cccttctgct tggttatacc ggcacgctcg cgatcggacc gtcgctttat
     3721 aaaaatgccg gctatgatcc gcgcaaggat tttgcgccga tcggcctgat cggcaacgct
     3781 cccaattcgc tggtggtgca tccttcgttc cccgccagga ccgtcactga gctgatcgcc
     3841 tatgcgaagg ccaatcccgg caaggtcaat ttcggctctg ccggcgccgg cacggccagc
     3901 cacatcaccg gcgaatattt tgcgcgttcg gccggcatta cgctggtgca tattcccgac
     3961 aaaggc
//
LOCUS       DATNOI010000125         9475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2613575,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000125 DATNOI010000000
VERSION     DATNOI010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9475
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2613575"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VN968_04610"
     CDS             <1..185
                     /locus_tag="VN968_04610"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HXL28555.1"
                     /translation="GTIKAARTRLAMVDIGGLVVRDVDAMVLPDAALSENLLGLSFLS
                     KLKRFEYANGKLVLEQ"
     gene            318..1817
                     /locus_tag="VN968_04615"
     CDS             318..1817
                     /locus_tag="VN968_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HXL28556.1"
                     /translation="MFPKPKSVLVPNTYAYESEPMVKATGFREYDARWLFGKEINLMG
                     IQALGMGLGALIAELGAKQEVVTGHDFRGYSASIKYALISGLQAAGCKVHDIGLCMTP
                     MAYFAQFELDVPCVAMVTASHNDNGWTGVKMGANRPLTFGPDEMTRLKEIVLNADFRN
                     KVGGSYQFHENFPARYIADLTKRPKLRRKLKVVVACGNGTAGAFAPQVMQAIGCEVVP
                     LDTELDYTFPKYNPNPEDMEMLHAIRDAVLEHKADVGLGFDGDGDRCGVVDNSGEEIF
                     ADKVGVMLARDMSAITKDAQFVVDVKSTGLFVTDPVLQKQGAKVTYWKTGHSYMKRRT
                     HELGALAGFEKSGHFFFNAPLGRGYDDGLISAIAICEMLDRAPGKSMADLKNALPKTW
                     SSPTMSPHCPDEAKYGIVDSVVKHFEAMQGKGEKVAGQPIRDLVTVNGVRVTVEDGSW
                     GLVRASSNKPELVVVVESPVSEQRMRDLFEAMDSVLRTHPEVGEYNQKI"
     gene            complement(1999..3468)
                     /locus_tag="VN968_04620"
     CDS             complement(1999..3468)
                     /locus_tag="VN968_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL28557.1"
                     /translation="MNSGSKDLDALRETASKALISLLWLHVPIAAVIGMARGTDWLMP
                     TILMIAFAAAATLSWRSVGNGLSTHLVIAVALMGGVSVFTFQLSGHAWQVDSHMYFFA
                     ALASLAAYCDYRPIIAGTIAVALHHLALNFILPAAVYPGGADFGRVVLHAVILLIEAG
                     VLIWLARKLSQLFETTTQKTAEAEAANAAEARANANRAEADLKAKQERDAAMRELAAG
                     FERKVGHIVEAVAVAAREMQGMSSSMSNSSGEAARQTAAAASASTQASLNVGTVASAA
                     EELTASISEIAHQATRSASVTGKAADEARRTNTVVEGLLTGTQKIGEVVTLIQNIASQ
                     TNLLALNATIEAARAGEHGRGFAVVASEVKALANQTAKATEEISTQIQSIQTATGEAV
                     SAIQAIGGTIAEINEISTAIAQAVGQQGTATREISGNVQQAANGTREVNDNIVGVAKA
                     SGELGSSASKLLEAANGLSSQSERLKLEVDSFLGSVRAA"
     gene            complement(3471..3863)
                     /locus_tag="VN968_04625"
     CDS             complement(3471..3863)
                     /locus_tag="VN968_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28558.1"
                     /translation="MSGFHPPLVLSQYPFGMGRTQNTTTSSWPAWLSATALLLVSVGA
                     VAALSLKVRPGAEVVAVAFPPWWSSRQIFEAAASAHAAIVRTTAITAILVVRPDAREG
                     LTRLREAGAWLTIDPQAIAGCFNIDKEI"
     gene            complement(3967..4467)
                     /locus_tag="VN968_04630"
     CDS             complement(3967..4467)
                     /locus_tag="VN968_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXL28559.1"
                     /translation="MTDLAVHVLEPNRRLDAIDRKILMVLQEDASLSVAEIGDRVGLS
                     STPCWKRIQRLEADGVILRRVALVDQNKIGLGISVFVSVESSDHSETWLRTFADAVSA
                     MPEVMELYRMAGDVDYMLRVVVADMQSYDVFYKKLIGAVPLKNVTSRFAMEKIKSVTA
                     LPVPAA"
     gene            complement(4536..5501)
                     /locus_tag="VN968_04635"
     CDS             complement(4536..5501)
                     /locus_tag="VN968_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HXL28560.1"
                     /translation="MNFHDAAAPKIAPRILCIGMPVRDLTFRVQGLPARGSKEHASHF
                     DEICGGNALNGAIGIVRLGGRASICGPMGDARETTSRYIFEKLADEGIDTRHIVHMPG
                     LVTPISNIMIDPSGERTIVTFRDPELWKVHLPDTDSLLEDCDAILTESRCAAFCTDLC
                     VEARRRGIPVIVDVDRAMSLREGLLTASSHLVFSSEPLQETAGVAEDGEALKKIAKLT
                     PSFLAGTRGALGTIWLDEHQNLQQTPAFAVHTVDTLGAGDVFHGAFALAITENQDLPE
                     ALRFASAAAALKCTRFGGAFAAPQRAEVDELLSHNQTPGPARVDQ"
     gene            complement(5583..6533)
                     /gene="nudC"
                     /locus_tag="VN968_04640"
     CDS             complement(5583..6533)
                     /gene="nudC"
                     /locus_tag="VN968_04640"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HXL28561.1"
                     /translation="MTAFDSFPLGQPAFVTHILDRAAHLRGNDEKLFALEGRRDARAY
                     VVYRDSLVVKQEADGPRALLTIDEALKFGANPGTIFLGLRDGAAVFGMGIAAAAVEKL
                     LTRDDAAVTELRGMAMQGVVPPDQLSAIAMAKSMVSWHQRHGYCANCGARTAMKEGGW
                     KRDCPNCKAEHFPRTDPVVIMLVASGDKCLLGRQKQFPAGMWSCLAGFVEAAETIEDA
                     VRREIFEEAGIRCTDVNYYMTQPWPYPSSLMIGCTARATSEDIIVDRAELEDARWFGR
                     DEATLMLRRQHPDGLAGPHPFAIAHHLLGRWLHVGPDARA"
     gene            complement(6539..6964)
                     /locus_tag="VN968_04645"
     CDS             complement(6539..6964)
                     /locus_tag="VN968_04645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HXL28562.1"
                     /translation="MPTDAPAWSLHSRLKEDTIDIGDLPLCRVLVIKDAHYPWLLLVP
                     RRPDAVEIIDLDEVEQAQLMTEISRVARSLKEITKCDKLNIAALGNLVPQLHIHVIAR
                     RSGDAAWPRPVWGVMPPLAHDAEEVQNFISAMRRKIWLG"
     gene            7220..7316
                     /gene="ffs"
                     /locus_tag="VN968_04650"
     ncRNA           7220..7316
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VN968_04650"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            7437..9287
                     /locus_tag="VN968_04655"
     CDS             7437..9287
                     /locus_tag="VN968_04655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HXL28563.1"
                     /translation="MTEAGIPVTPPTNEPDSAPRGGFGLGGPPEAGKHYRVLARKYRP
                     SSFEDLIGQDAVVRTVSNAFETGRIPQAWILTGVRGVGKTTTARILARALNYELPDGS
                     VKGPSIHMPKLGVHCQAIMESRHMDVLEMDAASHTGVDDVRQINDSVRYAPASARYKV
                     YIIDEVHMLSNQAFNAFLKTLEEPPEHVKFVFATTEIRKVPVTVLSRCQRFDLRRVEA
                     DVLMGHLANIAARENVEAEPEALGIIARAAEGSVRDSLSLFDQAIAHAAGVVRADAVR
                     QMLGLADRTRVIDLFDSLARGDIASAFKEFREQYDTGADPIVVLSDLAEFVNFVTRVK
                     IVPATADNVAFGETERLRARDFAAKLSMRVLSRMWQMLLKGITEVQTATRPAAAAEMV
                     LVRIAYVADMPTPDEAIRMLEQNGGASPVVAGAAASRSAAAPTVASMQSSAARASAPR
                     ASAEASARPQLIAPVSDAQSAPAARKISSFPELVALAAEKRDLLTKAALEADVRLVRI
                     EDGRLELAMERSAARTLINDLSRKLEQWTERRWTVIVSNEPGQPTLRSQNELQKSQRE
                     RAAESDPRVQEVLTRFPGARVVEVRKLAPEPPESDASVEDPAEISDSDDQ"
     gene            9309..>9475
                     /locus_tag="VN968_04660"
     CDS             9309..>9475
                     /locus_tag="VN968_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXL28564.1"
                     /translation="MADFLGMMKQAAQLQSKMQAMQEELGHVEVEGISGGGLVTVRMT
                     AKMQVKAVKIDP"
BASE COUNT         1770 a         2928 c         3026 g         1751 t
ORIGIN      
        1 acggaaccat caaggcggcg cgcacccggc ttgcgatggt cgatatcggc ggattggtgg
       61 tgcgcgacgt cgacgccatg gtgctgcccg acgcggcgct gtcggaaaac ctgctcggcc
      121 tgtcgttcct gtcgaagctg aaacggttcg aatacgccaa cggcaagctg gtgctggagc
      181 agtaagggct gcctcgccgc cgatccgccg ccattaatct aatcgccaca aaccggctgt
      241 cccgccttcc gctgggcctg cgcattcgct atgcctaagg tgcatttccc ggtcccattt
      301 caccgcaagg catcttgatg tttccgaagc cgaaatccgt actggtgccg aatacctatg
      361 cctatgaatc cgagccgatg gtgaaggcca ccgggtttcg cgaatacgac gcccgctggc
      421 tgttcggcaa ggaaatcaac ctgatgggta ttcaggcact gggcatgggg ctcggggcgc
      481 tgatcgcgga actcggtgcc aaacaggaag tggttaccgg gcatgatttt cgcggctatt
      541 cggcgtcgat caaatacgcg ctgatttcag ggctgcaggc cgccggctgc aaggtccatg
      601 acatcggcct gtgcatgacg ccgatggcgt atttcgcgca attcgaactc gacgtgccct
      661 gcgtcgcgat ggtgacggca tcgcataacg acaatggctg gaccggcgtc aagatgggcg
      721 ccaaccggcc gctcaccttc ggccccgacg agatgacccg cctgaaggag atcgtgctca
      781 atgccgattt tagaaataag gtcggtggct catatcaatt ccatgagaat tttccggcgc
      841 gctatatcgc ggatttgaca aaacgcccaa aactgaggcg caagctgaag gtcgtggtgg
      901 cctgcggcaa cggcaccgcg ggggccttcg cgccgcaggt gatgcaggcg atcggttgcg
      961 aggtggtgcc gctcgacacc gagctcgact acacctttcc gaaatacaat cccaaccccg
     1021 aagacatgga aatgctgcac gccatccgcg acgcggtgct ggaacacaag gccgatgtcg
     1081 gcctcggctt cgatggcgac ggcgaccgtt gcggcgtggt cgacaattcc ggcgaggaga
     1141 ttttcgccga caaggtcggc gtgatgctgg cgcgggatat gtccgccatc accaaggatg
     1201 ctcagttcgt ggtcgacgtg aagtcgaccg gactttttgt gacagatccg gtgctgcaaa
     1261 agcagggtgc caaagtaacc tattggaaga ccggtcactc ctacatgaag cgccgcaccc
     1321 atgaattggg cgcgctggcg gggttcgaga aatccggtca cttcttcttc aacgcgccgc
     1381 tcggccgcgg ctatgacgac ggcctgatct cggcgattgc gatctgcgag atgctcgatc
     1441 gtgcgcccgg caagtcgatg gcggatttga aaaacgcgct accgaagacg tggtcgtcgc
     1501 cgacgatgtc gccgcattgc ccggacgagg ccaaatatgg catcgtcgat agcgtggtga
     1561 aacacttcga ggcgatgcag ggcaaaggcg agaaggtcgc gggacaaccg atccgcgatc
     1621 tcgtcaccgt caatggcgtg cgcgtcacgg tcgaggacgg cagctggggc ctggtgcgag
     1681 cgtcatccaa caagccggaa ctggtggtgg tggtcgagag cccggtcagc gagcagcgca
     1741 tgcgcgacct gttcgaggcg atggattcgg tgctgcgcac ccatcccgag gtcggcgaat
     1801 ataaccagaa gatttgaggg taccttactc gtcattgccg ggcatagccg tccgaaggac
     1861 ggcgtcgctt ccgctcgcct atgacccggc aatccatccg ttttgaaaag gcatttcgtg
     1921 aagatggatg gatacgcggg tcaagcccgc gtatgacggc catgaatgtc gatgcgctac
     1981 gcgtgcatac tcttccagct acgccgcgcg gacggatccc agaaaactgt cgacctccag
     2041 tttcaaccgt tcggattgcg atgacaggcc gtttgcggcc tcgagcagct tcgaggcgga
     2101 gctgccgagt tcgccggaag ccttggccac cccgacaatg ttgtcattga cctctcgggt
     2161 gccgttcgcg gcctgctgca cgttgccgga tatctcgcgt gtcgcggttc cctgctgtcc
     2221 gacggcttgc gcgatggcag tggagatttc gttgatttcg gcgatggtcc cgccgatcgc
     2281 ctggatcgcg ctgaccgctt cgccggtcgc ggtctgaatg ctctggatct gcgtcgaaat
     2341 ttcttccgtc gccttcgcgg tctgattggc cagcgccttg acttcgctcg ccacgacagc
     2401 gaatccccgg ccgtgctcgc cggcccgcgc cgcttcgatc gtggcgttca aggcgagcag
     2461 attggtctgg ctggcgatgt tctggatcag cgtcacgacc tcaccgatct tctgcgtgcc
     2521 ggttagcagc ccctcgacaa cggtgttggt gcggcgggcc tcgtcggccg cctttccggt
     2581 aacgctggcg gagcgggttg cctggtgcgc gatctcgctg atcgaggcgg tcagctcctc
     2641 ggcagccgag gccacggtgc cgacattgag cgaagcctgt gtcgatgcgg aggcggccgc
     2701 ggccgtctgt cgcgcggctt ccccgctgct gttgctcatc gacgaggaca tgccctgcat
     2761 ttcgcgagcc gccaccgcga cggcctcgac gatgtggccg accttgcgct cgaagcccgc
     2821 ggcgagttcc cgcatcgccg cgtcacgttc ctgcttggct ttcaggtccg cctcggcccg
     2881 gttggcgttg gcgcgtgcct cggcggcgtt tgcagcttcc gcctcggcgg tcttctgcgt
     2941 tgtcgtctcg aacagctgtg agagcttgcg ggcaagccag atcaatacgc cggcctcgat
     3001 aaggagaatg acggcgtgaa gcacgacgcg cccgaaatcg gcgccgccgg gataaactgc
     3061 cgccggcagg atgaaattga gcgccagatg atgcagcgcg accgcgattg ttccggctat
     3121 gatcggccga tagtcgcaat aggcggccag ggacgccagg gccgcgaaga agtacatgtg
     3181 cgagtcgacc tgccaggcat gtcccgacaa ctggaacgta aacaccgaaa ctccgcccat
     3241 cagcgcgacc gcgatcacga gatgggtcga aagcccgttg cccacgctgc gccaggacaa
     3301 ggtggccgcc gcggcaaatg cgatcattag aatggtcggc atcagccaat cggtgccgcg
     3361 cgccatgcca atgaccgctg cgatcggaac atgcaaccac agcaacgaga tcaacgcctt
     3421 gctggcggtt tcgcgcaaag cgtccaggtc cttgctaccg ctattcatgc tcagatttcc
     3481 ttgtcaatgt tgaagcagcc ggcaatggct tgggggtcga tggtcagcca tgcgccggcc
     3541 tcacgcaaac gggtcaggcc ttcacgtgcg tcgggcctga ccacgagaat ggcggtaatg
     3601 gcggttgttc tgacgatggc ggcgtgagcc gaggcggcgg cttcgaaaat ctgtcgggag
     3661 gaccaccacg gcggaaaggc aacagccacg acttcagcgc cgggacgaac cttcagcgag
     3721 agtgctgcga ccgcaccgac gctcacgagc aataaagccg tggcgctgag ccaggctggc
     3781 caactcgacg tcgtggtgtt ttgtgtccgc cccatcccga agggatattg agacaagact
     3841 aatggtggat gaaaacccga caccgtaaaa ctacgggact gtattgaggt accgtcccac
     3901 aacacgcagt ctaggcgctg cgatcccagg caagtgctta ccgcgatatc cggccggcgc
     3961 ctatatctac gcagccggca ccggcaatgc ggtgaccgat ttgatcttct ccatcgcaaa
     4021 gcgtgacgtg acgttcttca gcggcaccgc gccgatcagc ttcttgtaaa acacgtcata
     4081 gctctgcatg tccgcgacca cgacgcgcag catgtaatcg acgtcgccgg ccatgcggta
     4141 cagttccatc acctccggca tcgcgctgac cgcgtcggcg aaggtccgaa gccaggtctc
     4201 ggagtgatcg gagctctcga ccgacacgaa aaccgagatg ccaaggccga tcttgttctg
     4261 gtcgaccagc gcgacgcggc gcaggatcac cccgtcggct tccagccgct gaattcgctt
     4321 ccagcacggc gtcgatgata gtcccacccg gtcgccgatc tcggcgacgg aaagcgaggc
     4381 gtcttcctgc aataccatca ggatcttgcg gtcgatggcg tcgaggcggc gattgggctc
     4441 gagaacgtga acggcgaggt cggtcatggg aaactttgtt ccatttatga ggccattact
     4501 cctcatatat agaaaattct tctaacacaa gcccattatt ggtcgacccg ggccggaccg
     4561 ggggtctggt tgtggctcaa aagctcgtcc acttcagcgc gttgcggagc ggcaaaagca
     4621 ccgccgaaac gggtgcattt cagcgccgcg gcggcggaag cgaaccgaag cgcctcgggc
     4681 agatcctggt tctcggtgat ggcgagggcg aaggcgccat ggaacacgtc gccggcgccc
     4741 agcgtatcca cggtgtgcac cgcgaaggcc ggcgtctgct gcaggttctg gtgctcgtcg
     4801 agccagatcg tgcccagcgc gccccgcgtt cccgccagga acgacggggt caattttgcg
     4861 attttcttga gcgcctcgcc gtcctcggcg accccggcgg tctcctgcag gggttcgctc
     4921 gagaatacca ggtgcgacga ggcggtgagc aacccctccc gcagcgacat cgcacggtcg
     4981 acatcgacga tgacagggat gccgcgccgg cgagcctcga cgcagagatc ggtgcagaac
     5041 gccgcgcagc ggctttcggt caggatggcg tcgcagtcct ccagcaggct gtcggtgtcg
     5101 ggcaggtgaa ccttccacaa ttccggatcg cggaacgtca cgatggtgcg ctcgccgctc
     5161 gggtcgatca tgatgttcga gatcggcgtc accaggcccg gcatatgcac gatgtgccgg
     5221 gtgtcgatgc cctcatccgc gagcttttcg aagatatagc ggctcgtggt ctcgcgggca
     5281 tcgcccatcg ggccgcagat cgaggcacgg ccgccgaggc gcacgattcc gatggcgcca
     5341 ttgagtgcgt tgccgccgca gatttcgtcg aaatggctgg cgtgctcctt ggaaccgcgc
     5401 gctggcaggc cctgcacgcg aaacgtcaga tcgcgcaccg gcatgccaat gcacagaatg
     5461 cgcggagcga ttttcggcgc ggcagcatcg tggaaattca ttcgggagtc cgtcatgtcg
     5521 gctccgccaa ttgccggaac caggatccgg gggtaatcga ttgaaccatg gctgagttcc
     5581 cgctatgcgc gcgcgtccgg gcccacatgc agccagcggc cgagcaaatg gtgtgcaatt
     5641 gcaaatggat gcggaccggc gaggccgtcg ggatgctgcc gtctcagcat caaggtcgct
     5701 tcgtcccggc cgaaccatcg cgcgtcttcc agttccgcgc ggtcgacaat gatatcctcg
     5761 ctggtggccc gcgcggtgca tccaatcatc agggatgacg gatagggcca tggctgcgtc
     5821 atgtagtaat tcacatcggt gcagcgaatc ccggcttcct cgaagatttc gcgccggacc
     5881 gcgtcctcga tggtttcggc ggcttcgaca aatccggcca ggcacgacca catgccggcg
     5941 ggaaattgct tctgccgccc cagcaggcat ttgtcgccgg aggccaccag catgatcacg
     6001 acggggtcgg tgcgcggaaa atgctcggcc ttgcagttcg ggcaatcgcg cttccagccg
     6061 ccttccttca tcgcggtgcg ggccccgcaa ttggcgcagt agccatggcg ctggtgccag
     6121 ctcaccatcg atttcgccat cgcgatcgcc gaaagctggt cgggcggcac cacgccctgc
     6181 atcgccatgc cgcgcaattc ggtcacggcg gcatcgtcgc gggtcagcaa tttttccacc
     6241 gccgcggctg cgatgcccat gccgaagacg gccgcgccgt cgcgcaagcc taagaagatc
     6301 gtgccgggat tagcgccgaa tttcagcgcc tcatcgatgg tcaagagcgc gcgtggcccg
     6361 tcagcctcct gcttcaccac cagcgagtcg cgatacacca cgtaggcgcg ggcgtcgcgg
     6421 cgcccttcca gcgcgaacag tttctcgtcg ttgccgcgca gatgcgcggc gcgatcgaga
     6481 atatgcgtga cgaaagccgg ctgccccagc ggaaacgaat cgaatgctgt catgtctctc
     6541 aaccaagcca gattttacgg cgcatcgcgc tgatgaaatt ctgaacctcc tcggcatcgt
     6601 gcgccagcgg tggcattacc ccccagaccg gacgcggcca ggcggcgtcg ccgctgcggc
     6661 gcgcgatcac gtggatgtgc agttgcggca ccagattgcc cagtgcggcg atattcagct
     6721 tgtcgcattt ggtgatctct ttcagcgagc gcgcgaccct tgaaatctcc gtcatcaatt
     6781 gcgcctgttc cacctcgtcg aggtcgatga tctccacggc gtcaggccgg cgcggcacca
     6841 gcaacagcca tgggtaatgc gcatccttga tcaccagcac gcggcacagc ggcaggtcgc
     6901 cgatatcgat ggtgtcttcc ttgagccgtg agtgcagcga ccaggcggga gcgtcagtgg
     6961 gcatgcatgt tccctcgtca tggccggcca tagcagtctg cgcagactgc gtaaacttgt
     7021 ccgcgatgcc gggcatccac gtcttccttg gtggcaacag gcaggacgtg gatggccggg
     7081 acatctagcg cgaagacgcg cttcgcgctt ctgcccggcc atcacggcaa ctaatgcctt
     7141 tccgtgttcc tgcacaagcc ctgcaaaccc ggtcccgcca cgcttgcttt ccggggcttt
     7201 tggccccaaa taaggaccgg gagattggcg gtggacgagc cactcgccaa ccgggtcagg
     7261 tccggaagga agcagcccta acgaggtccg gatcgggtcg ctcgtcagtc tcctacctgt
     7321 tttttcgaac gaatcgcgca aatggcggtg accaagagcg gtgacttggc ccgcgtccgg
     7381 tttgcgtcgt tgttccgcag gccggcttcg agagacaatc aattgcggat cgatcgatga
     7441 ccgaggctgg cattcccgta acacccccaa cgaacgagcc ggacagcgcc cctcgaggcg
     7501 gcttcggcct cggcggcccg ccggaagccg gcaagcacta tcgtgtgctg gcgcgaaaat
     7561 accggccctc cagtttcgaa gatttgatcg gccaggacgc cgtggttcgt accgtttcga
     7621 atgcattcga gaccgggcgg attccgcaag cctggattct gaccggggtg cgcggcgtcg
     7681 gaaaaaccac cacggcgcgg attcttgccc gcgcgctgaa ttacgaactg ccggacggtt
     7741 cggtgaaggg accgagcatc cacatgccga aactcggcgt gcattgccag gcgatcatgg
     7801 aaagccggca catggacgtg ctggaaatgg atgccgcatc ccataccggc gtcgatgatg
     7861 tccgccagat caatgacagc gtgcgctatg cgccggccag cgcgcgctac aaagtctaca
     7921 tcatcgacga agtgcatatg ctctcgaacc aggcgttcaa cgcgttcctc aagacgctgg
     7981 aagagccgcc ggagcacgtc aaattcgtgt tcgcgaccac ggaaatccgg aaagtgccgg
     8041 tcacggtgct gtcgcgctgc cagcgttttg acttgcgccg ggtcgaggcc gatgtactga
     8101 tgggccatct cgccaatatc gcggccaggg aaaacgtcga ggcggagccg gaggcgctcg
     8161 gcatcatcgc gcgcgcggcg gagggttcgg tgcgtgattc gctgtcgctg ttcgatcagg
     8221 cgattgcgca cgccgcgggc gtggtgcgtg ccgacgccgt gcggcagatg ctcggacttg
     8281 ccgatcgcac ccgggtgatc gatctgtttg attcattggc gcgcggcgac atcgcgagcg
     8341 cgttcaagga attccgcgag caatatgaca ccggcgccga cccgatcgtg gtgctgtccg
     8401 atcttgccga attcgtcaat ttcgtcaccc gcgtcaagat cgtgccggcg acagcggaca
     8461 atgtcgcatt tggcgaaacc gaacggttgc gcgcccgcga ctttgccgca aaactctcga
     8521 tgcgggtgct gtcgcggatg tggcagatgc tgctgaaggg catcaccgag gtgcagaccg
     8581 cgacgcgccc ggcggcggcc gccgaaatgg tgctggtgcg catcgcctat gtcgctgata
     8641 tgccgacgcc ggatgaagcg atcaggatgc tcgaacagaa tggcggtgca tcgccggtcg
     8701 tcgccggcgc cgcggcctca cggagtgcgg cggccccgac ggtggcctcc atgcagtcct
     8761 cggcggcacg cgcttcggcg ccacgcgcca gtgccgaggc gtcggcccgc ccgcagctga
     8821 tcgcgccggt ctctgacgcc cagtccgcac ccgcggcgcg caagatttcg agctttccgg
     8881 aactggtggc gcttgccgcc gagaagcgcg acctgctgac caaggccgcg ctggaggccg
     8941 atgtccggct ggtccgcatc gaggacggac ggcttgagct tgcgatggag cgcagcgcgg
     9001 cgcggaccct gatcaacgat ctttcccgta aactggaaca atggaccgaa cggcgctgga
     9061 ccgtgatcgt gtccaacgaa cccggacaac cgacgctgcg gtcgcaaaac gaattgcaga
     9121 agagccagcg cgagcgcgcc gccgaatccg atccccgggt gcaggaggtg ctgacgcgat
     9181 ttcccggagc cagggttgtc gaggtacgca agcttgcccc cgagccgccg gaatccgatg
     9241 ccagcgttga agatccggcc gagatttccg acagcgacga tcaatagcaa tttgatgagg
     9301 aacggttgat ggctgatttt ctcggcatga tgaagcaggc ggcgcaactg caatcgaaga
     9361 tgcaggcgat gcaggaagaa ctcggccatg tcgaggtcga gggcatctcg ggcggcggtc
     9421 tcgtcacggt gcgcatgacg gcaaagatgc aggtgaaggc ggtgaagatc gaccc
//
LOCUS       DATNOI010000126         2595 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2613335,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000126 DATNOI010000000
VERSION     DATNOI010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2613335"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VN968_04665"
     CDS             <1..564
                     /locus_tag="VN968_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL28565.1"
                     /translation="MVLHPHPQFHGTMNHQIIYQCYYAFAHRGFSVLRFNFRGVGRSQ
                     GSFDHGTGELSDAASALDWAQTINPEARACWVAGFSFGAWIGMQLLMRRPEVEGFISI
                     APPANLYDFSFLAPCPSSGLIVHGEKDAVVPPKDVNTLVEKLKTQKGIVIDQQIIPGA
                     NHFFDGKLEPLMQTVTGYLDMRLANVR"
     gene            complement(580..1254)
                     /locus_tag="VN968_04670"
     CDS             complement(580..1254)
                     /locus_tag="VN968_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXL28566.1"
                     /translation="MAARLRLISHKLCPYVQRAVIALSEKGVAFERIDIDLANKPDWF
                     LAISPLGKTPVLQVGDVAIFESAVILEYLEETQPHQVHPADPLRRAEHRGWIEFGSAV
                     LNDIAGFYAAADEATFKAKTVRLEQRFARLETRVAASPWFDGESFSLVDAVFGPVFRY
                     FDVFDGIGDFGILAGKPKLQRWRKALAARSSVQAAVSADYPSLLRDFLDRRNSWLSGL
                     QPRAAA"
     gene            complement(1317..2420)
                     /locus_tag="VN968_04675"
     CDS             complement(1317..2420)
                     /locus_tag="VN968_04675"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HXL28567.1"
                     /translation="MMLTAIGLMSGTSLDGVDVALIETDGRRVKAFGPTGYRPYTQDE
                     RGLLRQALTEAVNLPQRDARPGILREAEHAVTVAHAEAVAAFTAQNRLSREDVDIVGF
                     HGQTVLHRPAQKMTVQIGDATALARAIHIPVMHDFRAADVAAGGQGAPFVPVYHRALA
                     QSLERQGPMVVVNIGGVSNITYIDGADTLIACDTGPGNALLDDYMYRTMSQAFDCEGR
                     MAAQGAPDEAWVTRALEHPFFALPPPKSLDRNDFALLALPEMRPADGAATLTAFTAAA
                     IARIAPLLPKLPLSWIVVGGGARNLTMVRMLRERVAPATVEAGDALGWSTDSIEAQAF
                     GFLAARGLKGLPLSYPTTTGVPMPMTGGVIARP"
BASE COUNT          500 a          806 c          809 g          480 t
ORIGIN      
        1 atggtgctgc atccgcatcc gcagtttcac ggcacgatga atcaccagat catctaccag
       61 tgctactacg cgttcgcgca tcgcggattt tcggtattgc gtttcaattt ccgtggcgtc
      121 ggccgcagcc agggatcgtt cgatcacggc accggtgaac tgtcggatgc ggcgtccgcg
      181 ctcgattggg cgcagaccat caatccggag gcccgcgcct gctgggtcgc gggcttctca
      241 ttcggtgcgt ggatcggcat gcagctgctg atgcgccggc cggaggtcga aggttttatt
      301 tcgatcgcgc cgcccgccaa tctctacgac ttctcgttcc tggcgccctg cccgtcgtca
      361 ggcttgatcg tgcatggcga aaaggatgcc gtggtgccgc cgaaggacgt caatacgctg
      421 gtcgagaaac tcaagaccca gaagggcatc gtgatcgacc agcagatcat ccccggcgcc
      481 aaccactttt tcgacggcaa gctggagccg ctgatgcaga ccgtgaccgg ctatctcgac
      541 atgcggctcg ccaacgtgcg ctgagctcgc gggttggcct cacgcggcgg cgcgcggctg
      601 gaggcctgac agccaggaat tccgacggtc aaggaaatcg cgaagcagcg atggatagtc
      661 cgcactcaca gccgcctgca ccgatgaccg cgccgcgaga gccttgcgcc agcgttgaag
      721 ttttggcttg ccggccagaa ttccgaaatc gccgatcccg tcaaacacat cgaaatagcg
      781 gaacaccggg ccgaagacgg cgtcgaccag cgaaaaactt tcgccgtcaa accatggcga
      841 ggcggcgacg cgcgtctcga gccgcgcaaa tcgctgttcg agtcggactg tcttggcttt
      901 aaatgtcgct tcgtccgccg cggcgtaaaa accagcgatg tcgttgagga cagccgaccc
      961 gaattcgatc cagccgcgat gctcggcgcg cctcagggga tcggccggat gcacctgatg
     1021 cggctgggtc tcctcgagat attccaggat gaccgcggat tcgaaaatcg cgacatcgcc
     1081 gacctgcagc accggcgtct tgcccagcgg tgagattgca aggaaccaat ccggcttgtt
     1141 ggcgaggtcg atatcgatcc gctcgaaggc gacgcccttt tcgctcagcg caatcaccgc
     1201 gcgctgcaca tagggacaga gcttgtggct gatcaatctt aggcgcgcgg ccataacaaa
     1261 tctcacagat taatacattt gcatataact acatgcaatt gcatgaagct gtcaagtcac
     1321 ggccttgcga tcactccgcc ggtcatcggc attggaacac cggtagtagt gggatagctg
     1381 agcggcaggc cttttaagcc ccgcgctgcc aggaagccaa aagcctgggc ctcaatggag
     1441 tctgtcgacc agcccagcgc gtcgccggcc tcgaccgttg ccggcgccac tcgctcgcgc
     1501 aacattcgga ccatggtgag attgcgcgct ccgccgccga ccacgatcca gctcaacggc
     1561 agcttcggca gcaacggagc gatgcgggcg atcgctgcgg ccgtgaatgc cgtcagtgtc
     1621 gccgccccgt cggcgggacg catctcgggt agcgccagca aagcaaaatc gttgcggtcc
     1681 agcgatttgg gaggcggcag ggcaaagaac ggatgctcca gtgcgcgggt gacccatgcc
     1741 tcgtcggggg ccccttgggc tgccatgcgg ccttcgcaat cgaaggcctg gctcatggtg
     1801 cgatacatgt aatcgtcgag cagtgcgttg ccgggcccgg tgtcgcaggc gatcagggtg
     1861 tcggcgccat cgatataggt gatgttggag acgccgccga tattaaccac gaccatcggg
     1921 ccttgccgct caagcgactg ggcgagcgcg cggtgataga ccggtacgaa cggcgcgccc
     1981 tgcccgccgg cggcgacatc ggcggcgcgg aaatcatgca tgaccgggat atggatggcc
     2041 ctggcgagtg cggtggcatc gccgatctgc acggtcattt tttgcgccgg ccgatgcagc
     2101 acggtctggc cgtgaaagcc gacgatatcg acgtcctcgc gcgagaggcg gttttgcgcg
     2161 gtaaaggctg ccaccgcttc ggcatgcgcg accgtgaccg cgtgctcggc ctcgcgcagg
     2221 atgcccggcc gggcatcgcg ttgcggcagg ttcacggctt ccgtcagggc ctggcgcaac
     2281 agcccacgtt cgtcttgggt gtaaggccgg tatccggttg gtccgaacgc cttcacccgc
     2341 ctgccgtcgg tttcgatcaa ggcgacgtcg acaccatcga gcgaggtgcc gctcatcaga
     2401 ccgatcgccg ttaacatcaa agtcatggtg cttccgcgag acgcccctgc cctgaacgcc
     2461 cggcttgtgt caaacaagca catcttataa tgccacagcg cgccggccgg cggcagccta
     2521 tttcgccatg gtttcgcaac tgcttgagaa taacgtgaaa aaataagaat gatcagagtc
     2581 gcaccgaaat gactg
//
LOCUS       DATNOI010000127         6932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1378972,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000127 DATNOI010000000
VERSION     DATNOI010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378972"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..978)
                     /locus_tag="VN968_04680"
     CDS             complement(1..978)
                     /locus_tag="VN968_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL28568.1"
                     /translation="MRRLPTVMVATLVGLAWAGMAAAQNYPTRAMTMIVPFPAGGATD
                     TLARFLAEKMRAVLGQPVIIENVAGAAGSLGVGRAVRSPADGYTLSIGTSTTHMLTGG
                     LYALQFDLLKDLEPVIQIGSEPLLIVGRKSLPADDLKGLIAYLKANPDKASVGIAGVG
                     ATGHLTGISFQKETGTRFQFVPYRGNAPAMQDLLAEQIDFMIEPSSNFKSLVGAGSVK
                     PYAITGRTRLPSSPDIPTADEAGVAGFFASLWYGLWVPKDTPKDIIAKLNATMVQMLA
                     DPSVQKRFDELGIQITPLAQQSPEALRGFQKAEAERWWPIIKASNLKAE"
     gene            complement(1060..2820)
                     /locus_tag="VN968_04685"
     CDS             complement(1060..2820)
                     /locus_tag="VN968_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HXL28569.1"
                     /translation="MVTTSRYLEAPRRLRAFVRAHETSLVVLAGLIGTTGGLVVVAMS
                     MAVAGLHALLFDISIRERLSSQPSIEPLHALLVPSLGGLVLGLAFLLLLRWRPAREID
                     PIEANALHGGRMSFRGSVIVALQTVWSSGVGASVGLEAGYTQLASGLAASLGRGFHLR
                     RADQRIMVGCGAAAAIAGAFGAPLAGAFYAFELVIGGYTPASLTPVGVAAVAGYFVTH
                     GFAALSLGIGVGPVGDIRGRDLAIAALLGILAALFGIVIMRGVALCETLLSKTGLWPP
                     LRPALGGLAVGLLALLTPQVMSSGHGALHFAGIVSMPLTFIAVVFVLKAIASVISLGS
                     GFRGGLFFATLLLGVLGGRLFAAGFDTIWPGLNLDPNVYAIVGMSALSASVIGGPLTM
                     SFIALESTGNLWLTTAVLVAVIISTQITRELFGYSFATWRLHLRGETIRSAADVGWIR
                     DLTVGRLMRQDLTTVSADMRIEQFRGKFPLGSKNRVVAVDAAGRYVGLALVAEAHAPD
                     IDATKGLAGILHYRDVVLYPVMNIQEAIAVFDAAEAESLVVVETGGEQRPIGILSEAH
                     AMRRYAEESEQRRREAVGDI"
     gene            3015..3572
                     /locus_tag="VN968_04690"
     CDS             3015..3572
                     /locus_tag="VN968_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXL28570.1"
                     /translation="MNAHVSQGGWPVLVLNADFRPLSYYPLSLWSWQDAIKAVFLDRV
                     NIVEHYDRAVRSPSFEIQLPSVVSLKSFVKPTTHPAFTRFNVFLRDRFVCQYCSANDD
                     LTFDHIIPRSKGGQTTWENVVAACSPCNLRKGNLTPQQARMFPRQLPFAPTVHQLHRN
                     GRLFPPNYLHDSWLDYLYWDTELDP"
     gene            3801..4922
                     /locus_tag="VN968_04695"
     CDS             3801..4922
                     /locus_tag="VN968_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXL28571.1"
                     /translation="MHGILRAAVCIVLAVLTSGVRAQQAKSIQGVELHERDALNAGTV
                     TVITAPIGGPMSIMGSDMAAVLDDGDKLRVLPVLGKGSAQNLIDIIRLKNIDMGFVTT
                     DALEFVKTESNIPDIAKRVRYIAPLFYNDIHIVARQEIKTLEDLNGKRIFAERSIGLP
                     AARAIFRRMGIEADIDSQTDPDGGLQKLLNGERDAWVASVSKNAPVIKNIKNEGGKFH
                     LLEIPYQRPLQDIYLPSDFSSEEYPNLVPQGSRVDTVAVSTMLLVYNWPLESDRYRRV
                     ARFVDALFDKLPLFRQPPRHPKWRDTVISTNIPGLERFRAAQDWLDRSKEAMPASPPP
                     ARAEEFDRIVVQRNSGRKPAPEDAAKVYSDFLRWRDGQR"
     gene            complement(4996..6414)
                     /locus_tag="VN968_04700"
     CDS             complement(4996..6414)
                     /locus_tag="VN968_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXL28572.1"
                     /translation="MDNRSDIWRGVDAIKPRFITLSDKVWAMPEVCYTEARSAGEHLA
                     ELRHQGFRITENVAGIPTAAMGEWGEGGPVIAFLGEYDALPGLSQEAGVAEPRPLEVG
                     GHGHGCGHNLLGSAALLAATAAKDWLAAHKVPGRVRYYGCPAEEGGAAKTFMVRSGAF
                     DDADIAITWHPSSFWEVVVTPSLANTRADFIFTGRTSHAAASPHLGRSALDAVELMNV
                     GVNYMREHMPSDARVHYALLDTGGIAPNVVQAHARVRYSIRARDLPGMNELVERVHKI
                     AQGAALMTETRMEMRIISAVSNVLPNTPLEQSLHRIMEDLGPPRFDEADQEFAKKIRS
                     TLTEKDIASVYHAIGMDPTDRPLADFIVPLDARRNPLIGSTDVGDVSWVVPTVQVHSP
                     TVAIGTPFHTWQVVAQGKSPAAHKAMVQAAKAMAGLGVKALNEPDLIAAAKADLKKRT
                     ARTPYVSPLPDNVAPPLDMSLT"
BASE COUNT         1315 a         2224 c         2165 g         1228 t
ORIGIN      
        1 ctattccgcc ttgaggtttg acgccttgat gatcggccac caccgttccg cctcggcctt
       61 ctggaacccg cgcagggctt ccggcgattg ctgcgcgagc ggcgtgatct ggatgccgag
      121 ttcgtcaaag cgtttttgca ctgagggatc ggcgagcatc tgtaccatgg tggcgttgag
      181 cttggcgatg atatccttcg gcgtatcctt gggcacccag aggccgtacc agagcgaggc
      241 aaagaagccc gcaacacccg cctcgtccgc tgtcgggata tccggcgacg aaggcaaccg
      301 cgtccggccc gtgatggcgt aaggcttgac gcttccggcg ccgaccagcg acttgaaatt
      361 cgacgacggc tcgatcatga aatcgatctg ttccgccaac agatcctgca tggccggcgc
      421 attgccgcga tacggcacga attgaaacct ggttccggtt tccttctgga acgaaatgcc
      481 ggtcagatga ccggtcgcgc cgaccccggc aatgccgacc gatgccttgt ccggattggc
      541 cttgagatag gcaatcagcc ctttcagatc atccgccggc aggctctttc tgccgacgat
      601 caaaagcggc tcgctgccga tctgaatgac cggttccaga tctttcaata gatcgaattg
      661 caacgcgtag aggccgcccg tcagcatatg cgtggtcgag gtgccgatgc tcagggtgta
      721 gccatcggcg ggggaccgga cggcgcggcc gacgccgagg ctgccggcgg ctcccgcaac
      781 gttctcgatg atgacgggtt gaccgagaac agcccgcatc ttctcggcca gaaatcgcgc
      841 cagggtatcg gttgctcccc cggcggggaa cggcacgatc atggtcattg cgcgcgtcgg
      901 atagttttgc gcggcggcca tgccggccca tgccaggccg acaagcgtcg cgaccatcac
      961 cgtcggcaat ctgcgcatgg ggttcccccg gggatgtgct tatgcgatgc acgttgaccc
     1021 tgcttggacc ctgcttgtcg cgcttttccg cggcgctgac tagatatcgc cgacggcttc
     1081 gcgccgccgt tgttcggatt cttccgcata gcgccgcatc gcgtgggctt cgctgagaat
     1141 tccgatcggc cgctgttcgc cgccggtttc gaccacgaca agcgactcgg cttccgcggc
     1201 gtcgaagaca gcgatggctt cctggatgtt catgacagga tacagcacga catcccgata
     1261 atgaaggatg ccggcaaggc ccttcgtcgc gtcgatgtcc ggcgcgtgcg cttcggccac
     1321 cagcgccagt ccgacgtagc ggcccgcggc gtcgaccgcg accacccggt tctttgagcc
     1381 gagcggaaat ttgccgcgga attgttcgat tctcatgtcg gcgctcacgg tggtcagatc
     1441 ctgccgcatc aggcgaccga ccgtcagatc gcggatccag cccacatcgg cggcgctgcg
     1501 gatggtctcg ccgcgcaggt gcaatcgcca cgtcgcaaac gagtagccga acagttcgcg
     1561 ggtgatctgg gtcgagatga tgaccgcgac cagcacggcc gtggtgagcc agagattgcc
     1621 ggtcgattcc agcgcgatga acgacattgt cagcggcccg ccgatcaccg acgccgacag
     1681 cgcgctcatg ccgacgatcg catagacgtt cggatcgagg ttgagcccgg gccagatcgt
     1741 gtcaaagccg gcggcgaaca ggcggccgcc gagcacgccg agaagcagcg tggcgaaaaa
     1801 caatccgccg cgaaagccgc tgccgagcga tatcaccgac gctatcgcct tcagcacgaa
     1861 cacgaccgcg atgaatgtca atggcatcga gacgatgccg gcaaaatgca gcgcgccgtg
     1921 ccccgacgac atcacctgcg gggtcagcaa tgcgagcaga cccaccgcga gaccgccgag
     1981 cgcagggcgc agcggcggcc agagtccggt cttcgacagc agcgtctcgc acagcgccac
     2041 gccgcgcatg atgacgatgc caaacaacgc cgcgaggatc ccgagcagtg cggcaatggc
     2101 aaggtcgcgc ccgcgtatat cgccgaccgg accgacgccg atgccaagcg acagcgccgc
     2161 gaatccatgg gtgacaaaat agcccgccac cgcggcgaca ccgaccggcg tcaggctggc
     2221 cggcgtatag cccccgatca caagctcgaa ggcatagaag gcaccggcaa gcggtgcccc
     2281 gaacgcgccg gcgatcgcgg cggcagcgcc gcagccgacc atgatgcgct gatcggcgcg
     2341 gcgcaggtga aatccgcggc ccagggaggc ggcaagcccg ctggcaagct gggtatatcc
     2401 tgcttcaagt ccgaccgaag cgccgacgcc gctggaccac accgtctgca atgccacgat
     2461 cacactgccg cgaaacgaca tccgcccgcc atgcagggca ttggcctcga tcggatcgat
     2521 ctcccgcgcc ggccgccagc gcagcaacag caggaaggcc agccccagca ccaacccgcc
     2581 aaggctcgga accaacaggg cgtgcagcgg ctcgatgctg ggctggcttg aaagccgctc
     2641 cctgatcgaa atgtcgaaca ggagcgcgtg aagcccggcg accgccatac tcatcgcaac
     2701 caccaccaat ccgcctgttg ttccgatcag ccccgcgagg acgaccaggc tggtttcatg
     2761 ggcacgaaca aacgcgcgca gccggcgcgg cgcctcaaga taacgtgacg tcgtgaccat
     2821 tcttgctgcg cccgtatgcg gccggaaccc gccgccagcc ccgatttagc ggtctttacg
     2881 ccgatcgcgc aatccagtcg caccaggcct gagcggcgga taagcctctc aacgctcttc
     2941 acaatcccgt cacgctgcct gtagtatgtc agttcatgct gcttcgcagc taaaacgggg
     3001 gtcaggagcg ttacttgaac gcacatgtct cgcaaggcgg ttggccggta ctggtgctga
     3061 atgcggactt ccggccgctc agttattatc cgctgtctct ctggtcgtgg caggacgcga
     3121 tcaaggcggt gttcctcgac cgcgtcaata tcgttgagca ctacgaccgc gcggtgcgca
     3181 gcccttcgtt cgagattcag ctgccgagcg tggtgtcgct gaaatccttc gtcaagccga
     3241 ccacgcatcc cgccttcacc cggttcaacg tgttcctgcg cgaccgcttc gtctgccagt
     3301 attgttcggc caacgacgac ctcaccttcg atcacatcat cccccgcagc aagggcggcc
     3361 agaccacctg ggaaaacgtg gtcgcggcat gttcgccgtg caatctgcgc aagggaaacc
     3421 tgacgccgca gcaggctcgg atgtttccgc ggcaactgcc gttcgcaccc acggtgcatc
     3481 agctgcatcg caacggccgg ctgttcccgc cgaactatct gcacgacagc tggctggatt
     3541 atctttattg ggataccgaa ctcgatccgt aggaagcggc cgacggtttt gttgtcgtgc
     3601 ttgttgtcgt ggggctcata aaagttttca cgccagcctg gcgcgcttcc cgccccgggc
     3661 gttgcactcc tcgatcgtgt caaacggcgc ctgctgggga cgaattccgc cgcgcgccaa
     3721 gcatcattga gtcaaaattc agccgacttt cgccgcatcg tgggctaccg acgctcgcga
     3781 actgttgaac agaggccgcc atgcatggaa tcttgcgcgc agccgtttgc attgtactcg
     3841 ctgtcctgac aagcggcgtc agggcgcagc aggccaaatc gattcaaggc gttgagttgc
     3901 acgaacgcga cgcgctcaat gccggcaccg tgacggtgat tacggcgccg atcggcggtc
     3961 cgatgtcgat catgggctcc gatatggcgg ccgtactcga cgacggcgac aaactcaggg
     4021 tgctgccggt gctcggcaag ggctcggccc agaatctcat agacatcatc cggttgaaga
     4081 atatcgacat gggctttgta acgaccgacg cgctggagtt tgtgaagacc gaatccaaca
     4141 ttccggacat tgccaagcgc gtccgctata tcgcgccgct gttctacaac gacattcaca
     4201 tcgtggcgcg ccaggagatc aagaccttgg aggatctcaa tggaaagcgg attttcgccg
     4261 agcgcagcat cggactgccg gcggcgcggg cgatcttccg acgcatgggc atcgaggcgg
     4321 acatcgattc gcagactgat cccgatggcg ggctgcagaa gctgcttaac ggcgagaggg
     4381 acgcatgggt cgcgagcgtt tcgaagaacg ctccggtcat caagaacatc aagaacgagg
     4441 ggggcaaatt ccatttgctg gagatcccct accagcggcc gctgcaagac atctatctgc
     4501 cgtcggactt ctcgtccgag gaatatccga acctcgtgcc gcagggcagc agggtcgaca
     4561 ctgtcgctgt ctcgacgatg ttgctggtat acaactggcc gctcgaaagc gaccgctaca
     4621 gacgggtcgc ccgcttcgtc gatgcgttgt tcgacaaact cccgctgttc cgacagcccc
     4681 cgcgtcatcc gaaatggagg gataccgtga tatcgaccaa catccccggc ctggagcgtt
     4741 ttagggccgc gcaggattgg ctcgacagat cgaaggaggc gatgccggcg agcccgcccc
     4801 cagcaagagc cgaggaattc gacaggattg tcgttcagcg aaacagcggc aggaagccgg
     4861 cgcccgaaga cgccgccaag gtttacagcg actttcttcg atggcgcgat ggacagagat
     4921 aacgttatcc gttgttcgca ccgcccgcgg cgcacggcgc atgcgaaaaa tttgttcgcc
     4981 aaatcaggtc tagggtcagg tcaaggacat gtccagcggc ggcgcgacat tgtccggcag
     5041 cgggctgacg tagggcgtgc gggcggtgcg cttcttgagg tcggccttgg cggcggcgat
     5101 caggtccggc tcgttaagcg ccttgacgcc gagccccgcc atggccttgg cggcctgcac
     5161 catggccttg tgggcggccg ggctcttgcc ctgcgccacc acctgccagg tgtggaacgg
     5221 ggtgccgatg gcgacggtcg gggaatggac ctgaacggtc gggacgaccc agctcacgtc
     5281 gcccacatcg gtcgagccga tcagaggatt tcgcctggcg tccaacggca cgatgaaatc
     5341 ggccagcggg cggtcggtgg gatccatgcc gatcgcatga tagaccgaag cgatgtcctt
     5401 ctcggtcagc gtcgaccgga tttttttggc gaattcctgg tccgcctcgt cgaaacgcgg
     5461 gggaccgaga tcctccatga tccggtggag ggattgctcg aggggagtgt tgggcaagac
     5521 attggagacc gcggaaatga tccgcatctc catcctggtc tcggtcatca gcgccgctcc
     5581 ctgcgcgatc ttgtggacac gctccaccaa ttcgttcatg ccgggcagat cgcgggcgcg
     5641 gatggaatag cgcacgcggg cgtgggcctg caccacattg ggggcaatgc cgccggtgtc
     5701 gagcagagca tagtggacgc gggcgtcgct cggcatgtgc tcgcgcatgt agttcacgcc
     5761 gacattcatc agttccaccg cgtcgagcgc gctgcggccc agatggggcg aggccgcggc
     5821 gtgcgaggtg cgcccggtga agatgaaatc ggcgcgcgtg ttggccagcg aaggcgttac
     5881 caccacctcc cagaagctgg acgggtgcca ggtgatggcg atgtcggcgt catcgaaggc
     5941 gcctgagcgc accatgaagg ttttcgcagc acccccctcc tcggcggggc agccataata
     6001 acgcacgcgc cccggcacct tgtgagcggc gagccaatcc ttcgcggccg tggcggcgag
     6061 cagcgcggcc gagcccagga gattgtggcc gcagccgtgg ccatggcctc ccacctcgag
     6121 cgggcgaggc tcggccacgc ccgcctcctg gcttagaccg ggtagcgcat cgtattcacc
     6181 gaggaacgcg atgacgggtc caccctcacc ccactcaccc atcgcggcgg tgggaatgcc
     6241 ggcgacattc tccgtgatgc gaaaaccctg atggcgcagt tcggccagat gttcgcccgc
     6301 cgagcgcgcc tcggtgtagc acacctcggg catcgcccag accttgtcgc tcaaggtgat
     6361 gaaacgcggc ttgatcgcgt caacgccacg ccaaatatcg ctgcggttgt ccatgctgcg
     6421 gtcccggatt gctgtcagag tgagatcgtt atcagcttcg ccagaccctg cctagcgccg
     6481 gccagggcga cctgccatgc ggccggcgcg aaggacatgc gtcgacgatc tcgcggcgcg
     6541 atgcgcccga gtcgtgcacg acgtttccct cgaatagata gagggagtgg ggaatgccgg
     6601 atgcccgctg cacccgcggt ctcgtgtgcg cttgtagtgg tagaaagcac acgagtaaca
     6661 acgagcacac cggaatcacc cggcattccc gcacgcaatg gttttaacgg tttacttcgt
     6721 gctctccccg gtgaccgggc tcttttgcca ccgtcgcttg cggatatggt tttgtctaag
     6781 cccggtaggg ccgacagaac atccgcaaac ttgacgccag cgtcggggcg tcaggaccac
     6841 acgacttcgc cgtacgcaac aacatctctc gtccgcgtgc tgttgatcgc tcacaggctc
     6901 atcacccgcc ctgcaatccc gttgcgcgca aa
//
LOCUS       DATNOI010000128         2541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2442860,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000128 DATNOI010000000
VERSION     DATNOI010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2442860"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VN968_04705"
     CDS             complement(<1..217)
                     /locus_tag="VN968_04705"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL28573.1"
                     /translation="MATAGVGTGQHLVAAAFMKAAGVKLLEVPYKGSPPAFTDLLAGR
                     IDLFFDSVAAGLPYVQSGQAKGIAVLSS"
     gene            385..843
                     /locus_tag="VN968_04710"
     CDS             385..843
                     /locus_tag="VN968_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HXL28574.1"
                     /translation="MARRKLFTIGYEQTPAKAVLDELERTGVKLLVDVRAVAASRRPG
                     FSKNQLTAGLDEGGISYLHLRGLGTPKDGRDAARSGQFDALHKIYAKHLKTPQAKEEL
                     DELSALVKTSGPVCILCYERDHLHCHRQWIAEIIEDRDGVKVENLVAPQV"
     gene            complement(995..1906)
                     /locus_tag="VN968_04715"
     CDS             complement(995..1906)
                     /locus_tag="VN968_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HXL28575.1"
                     /translation="MQRRYITVDVFTDRAFGGNPLAVILDAGGLSTAQMQAIASEFNY
                     SETTFVLPPQDPAHDARVRIFTVRSEIPFAGHPNVGTAFVLATQAAKPPARLVFEEKA
                     GLVPVEILIEDGKVVGAEFTAPQGLKKLTQLTAEHAAACASLSAADIRTDRHPPQIVS
                     VGLPFLVAELASRDALRRARPDADAFARTLPCDGSDAVYLYTRDVPTAEACDLQARMF
                     HPGSSGLSEDPATGSATAAAAALLADLSGEWDGELKLRIGQGVDINRPSLLLTRVRKA
                     NGVVMSAHVGGACVQMMEGTFRLAGEG"
     gene            complement(1982..>2541)
                     /locus_tag="VN968_04720"
     CDS             complement(1982..>2541)
                     /locus_tag="VN968_04720"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL28576.1"
                     /translation="TNSDIRHVAVMFVDFRSFTAGARSRSPQEVVDRLDGAFAVLVDI
                     LDRHGGIVNKFLGDGFLALFGAPFESEDAALRSVAAAREMLAANESFNQATSWPLQIG
                     IGIHFGEVVAGNIGSPRRKEYTVIGDTVNFAARLESLNKEFNSRLLISAAVRDALDEA
                     GSDAVSLGEVAIRGYDRPMTVWQLG"
BASE COUNT          489 a          825 c          814 g          413 t
ORIGIN      
        1 tggacgacag cacggcgatg ccttttgcct gccccgactg cacatatggc aggccggcgg
       61 cgacggagtc gaagaacaga tcgatacggc ctgcgagcag atcggtgaaa gccggcggcg
      121 agcccttgta aggcacttcc agcagcttca cgcccgcggc cttcataaac gcagccgcca
      181 ccaggtgctg accggttccg acgcctgcgg tcgccacgga tatggagccc ggctcttgtt
      241 tgcgagccga ctatgcgacg cagcgcggat ggcggcaacg gaggctgcgg ctggcttttc
      301 caacataatc tgagcggtta tggaaatcat tccaggactg acgtagcctt agagggatga
      361 catcaccaac gcctggacgc tgacatggca cgcagaaagc tcttcaccat cggctatgaa
      421 cagacccccg ccaaggccgt gctcgacgaa ctcgagcgca cgggagtaaa gctgctggtg
      481 gacgtgcgcg ccgtcgccgc ttcgcgccgg cccggctttt ccaagaacca gctcaccgcc
      541 ggactcgacg agggcggcat ttcatacttg catctgcgcg ggctgggcac gccgaaggac
      601 ggccgcgacg ccgcgcgcag cggccaattc gatgcgctgc acaaaatcta tgcgaagcac
      661 ctgaagacgc cgcaggcaaa ggaagaactc gacgagctgt cggcgctggt caagacatcc
      721 gggccggtct gcattctctg ttacgagcgc gatcacctgc actgccaccg gcaatggatc
      781 gccgagatca tcgaagaccg cgacggcgtg aaggtcgaga atctggtcgc gccgcaggtg
      841 tgattgtcat tccgggatgc gccaacgagt ccgcgcaaaa gcgcggcccg atgacaggct
      901 ccgcgcgagc ccggaatcca tagccaccgc ggggattatg gattccgggc ctgcgcctta
      961 cggcgcatcc cggaatgacg gcggaatcat ttgttcaccc ctccccggcc agccgaaacg
     1021 ttccttccat catctgcacg caagcgccgc cgacatgcgc ggacatgaca acgccgttcg
     1081 ctttgcggac gcgggtcagc agcaggctcg gcctgttgat atcgacgccc tgcccgatgc
     1141 gcagcttcaa ttccccatcc cactcgcccg acaagtcggc gagcagtgca gcggctgccg
     1201 cggtggcgct gccggtggcc gggtcttccg aaaggccgct cgatcccggg tgaaacatcc
     1261 gcgcctgcag gtcgcacgcc tcggcggtgg gaacatcgcg ggtatagaga tagaccgcgt
     1321 cgctgccgtc gcagggaagc gtgcgggcga atgcgtccgc gtcgggcctg gcccgccgca
     1381 gcgcgtcgcg cgaggctagc tccgccacca gaaacggcag cccgaccgag acgatttgcg
     1441 ggggatgccg gtcggttcgg atatccgccg ccgatagcga agcacaggcg gcggcatgct
     1501 cggcggtgag ttgcgtgagc ttcttgaggc cctgcggcgc ggtgaactcg gcaccgacca
     1561 ccttgccatc ctcgatcagg atctcgaccg gcaccaggcc tgctttttcc tcaaacacca
     1621 gccgcgccgg cggcttggcg gcctgcgtcg ccagcacgaa ggcggtaccg acattgggat
     1681 ggcccgcgaa cgggatctcg gagcggaccg taaagatgcg aacccgcgcg tcgtgcgccg
     1741 ggtcttgcgg cggcagcacg aaggtcgtct cggaataatt gaactcggac gcaatggcct
     1801 gcatctgggc ggtcgacagc ccgccggcat ccaggatcac cgccagcgga ttgccgccga
     1861 aggcgcggtc ggtgaaaacg tcgacggtga tatagcgccg ttgcatgtga gatctccggt
     1921 ttcgcctcag ccagaacatg atgcggattg gttcgtcaag cgcggcgaac gccctgcgtt
     1981 tctatcccag ctgccacacc gtcatcggcc ggtcatagcc cctgatcgcg acttccccga
     2041 gcgacaccgc gtcgctgccg gcctcgtcca gcgcgtcacg aacggcggcc gaaatcagca
     2101 atcgggaatt gaattccttg ttgagcgatt ccagccgggc ggcaaagttc acggtgtcgc
     2161 cgatcacggt gtactccttg cgccggggag aaccgatatt gccggcaacc acttcgccga
     2221 aatgaatgcc gatgccgatc tgcagcggcc acgacgtcgc ctggttgaag ctttcattgg
     2281 cggcgagcat ttcgcgcgcc gcggcgacgg aacggagcgc ggcatcttcc gattcaaagg
     2341 gcgcgccgaa cagcgctaga aatccgtcgc ccagaaactt gttcacgatg ccaccatggc
     2401 ggtcgaggat atcgaccagc acggcgaacg cgccgtcgag acgatccacc acctcctgcg
     2461 gcgaacgcga gcgggcgcca gcggtaaagc tgcggaaatc gacgaacatg acagcgacat
     2521 ggcggatgtc gctgttggtg c
//
LOCUS       DATNOI010000129         2121 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1378936,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000129 DATNOI010000000
VERSION     DATNOI010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378936"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1534
                     /locus_tag="VN968_04725"
     CDS             101..1534
                     /locus_tag="VN968_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXL28577.1"
                     /translation="MMIKRILTICAAAAAGAAASSLALAQGYPVPPAPVYSTAPLPYP
                     SGGYPDYRRAPTELDADLLEDDEALPNAQRSTALPPPGPGLSPDDPRYRRPIYSDRGA
                     PTGPILSPDDPRYGRPEGTPPVYSDRSAPTGPIVSPDDPRYGRPAGAPPVYSDRGAPA
                     GPILSPDDPRYGRPEGTPPVYSDRGAPAGPILSPNDPRYGRPDGPPPVIYSDRGNVDG
                     RIPGPGFNPNEDNRALRPPGAIGPPGPVTGSVQPPMGPDGRPMVLSSLPPEEQPEVGP
                     AQLAPNLRRQEVAFATKEPPGTLIVDTPHTYLYYVLGKGRAIRYGVRVGRDGFTWNGV
                     QKISRKAEWPDWHPPTEMIERQPYLPRFMAGGPGNPLGARAMYLGSTVYRIHGTNQPS
                     TIGKFVSSGCIGMLNEDVSDLFDRVKVGTRVVVFPGGPPPGTATASAQPVPGPAASAS
                     PAPPQMSGAPGTQPTVVPPLPAPVTVR"
     gene            complement(1676..>2121)
                     /locus_tag="VN968_04730"
     CDS             complement(1676..>2121)
                     /locus_tag="VN968_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL28578.1"
                     /translation="VIAWSPNLTPERCKEVGVGYATKEELFSTADIVTIHVVLSQRSR
                     GLVGASDLARMKPTSYLVNTARGPIVDEAALLQTLQQKRIAGAAVDVFSVEPLPVDHP
                     FRKLDNIVLTPHLGYVTEEGFRNHYSQMVEGIDAWFKGEPLRRLA"
BASE COUNT          332 a          732 c          664 g          393 t
ORIGIN      
        1 ggaatccggc cgaattacgc ggatttgtct tgaatgcgcc gctatcttcg gccatctgcc
       61 gttaacggtc cggtggcggc ttggggttta tacaagggcg atgatgatca aacgcattct
      121 gacgatttgc gctgcggcgg cggctggcgc ggcggcaagt tcgcttgcgc tcgcgcaagg
      181 gtacccggtt ccgcccgctc cggtctattc gacggcgccg ctgccttatc cgtccggcgg
      241 ctaccccgat tatcgccgcg ctcctaccga gttggatgcc gacttgctgg aagatgacga
      301 ggcgctgccg aacgcccaga ggtcgactgc gctgccgccg cccggcccgg ggctttcgcc
      361 ggatgatccg cgctatcggc gtccgattta ttccgaccgt ggcgcgccga ctggcccaat
      421 cctttcgccc gatgatccgc gctatggcag gcccgagggt actcctccgg tatactccga
      481 ccgtagcgca ccgactggcc cgatcgtttc accggatgat ccgcgctatg gtcgccctgc
      541 gggtgctccg ccggtttatt ccgaccgtgg cgcaccggcc ggtccaatcc tttcgccgga
      601 tgatccgcgc tatggcaggc ccgagggtac tcctccggta tactccgacc gtggcgcacc
      661 ggccggcccg atcctttcgc ccaatgatcc gcgttatggc cgtcccgacg gaccgccgcc
      721 ggtgatctat tcggatcgcg gcaacgtcga tggccgaatt cccggaccgg gcttcaatcc
      781 gaatgaagac aatcgtgcct tgcgcccacc cggggcgatc ggtcccccgg gcccggtcac
      841 cggatcggtg cagcctccga tgggtcccga cggcaggccg atggtgctgt cttcgcttcc
      901 gccggaggaa cagccggagg tcgggcctgc gcaacttgcg ccgaacctgc gccgacagga
      961 agtcgccttc gccaccaagg agccgccggg aacgctgatc gtcgatacgc cgcacaccta
     1021 tctctactac gtgctgggta aagggcgcgc gatccgttat ggcgtccgtg tcggccgcga
     1081 cggcttcacc tggaacgggg tacagaaaat ctcgcgcaag gccgaatggc cggattggca
     1141 cccgccgacc gagatgatcg agcgtcagcc ttatctgccg cgcttcatgg ccggtggacc
     1201 gggcaatccg ctcggtgccc gggcgatgta tctcggctca accgtctacc gcatccacgg
     1261 caccaaccag ccctcgacga tcggcaagtt cgtctcctcg ggctgcatcg gcatgctcaa
     1321 cgaggacgtc tcggacctgt tcgaccgcgt caaggtcggc acccgcgtgg tggtgttccc
     1381 aggcggcccg ccgccgggaa cggcaaccgc gtcggcgcag ccggtgcccg gacctgccgc
     1441 ctcggcgtcc cccgcgccac cgcagatgtc gggcgcgccc ggcacgcaac cgaccgtcgt
     1501 gccgccactg ccggcgccgg tgactgtgcg ctaactctcg ccactaattc agtcgtcatc
     1561 ctgaggtgcg cgctcttgcg cgcctcgaag gatgaccgcg cacgctgacc attcttcgag
     1621 gcgcgccaaa gatggctcgc acctgaggat gacgaacgtg ttatgcggaa tacagctacg
     1681 ccagccttcg cagcggctcg cccttgaacc aggcgtcgat gccctcgacc atctggctgt
     1741 agtggttgcg aaaaccctct tcggtgacgt agccgagatg cggcgtcagc acgatgttgt
     1801 cgagcttgcg gaacggatga tcgaccggca gcggttcgac cgagaataca tcgaccgcgg
     1861 cgcctgcgat cctcttctgc tgcagggttt gcagcaacgc tgcttcgtcg acgatcggcc
     1921 cgcgcgcggt gttgacgaga tagctcgtcg gcttcatgcg cgccaggtcc gaagctccca
     1981 ccagcccgcg cgagcgctgg ctcaacacca catggatggt gacgatgtcg gcggtcgaaa
     2041 acaattcctc cttggtggcg tagccgacgc cgacttcctt gcatcgctcc ggcgtcaggt
     2101 tcgggctcca cgcgatcacg t
//
LOCUS       DATNOI010000130         7835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1880810,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000130 DATNOI010000000
VERSION     DATNOI010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880810"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1238..1816)
                     /locus_tag="VN968_04735"
     CDS             complement(1238..1816)
                     /locus_tag="VN968_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HXL28579.1"
                     /translation="MTKDKPGTIQVKSFADHKVITQPNPLRKVLRRVADKDLDDPIAR
                     AEKALAGLSGEFKNWMSVEADRLAAAHAAILKTGFTDFSREELFRAAHDIKGDAATFG
                     FPSAAAAAESLCRIIEHAPNLDEVPSDLIAHHINAIQAIVRERTRLDTVSTANELSRQ
                     LRGIADEFLMHANRDRPEHLEAILAPSIAPGD"
     gene            complement(1857..2402)
                     /locus_tag="VN968_04740"
     CDS             complement(1857..2402)
                     /locus_tag="VN968_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL28580.1"
                     /translation="MFRIDFNKLRFLVCDDNPHMRRILRTLLHSFGAREVYESEDGAT
                     ALEMYSHYVPDIVITDWSMPIFDGLELAQMIRQPESKGNPYAPIIMLTGHSEKRRVTV
                     ARDAGVTEFLAKPISAKGLYQRILNVVANPRPFIKTKTYFGPDRRRNTANAYIGPERR
                     IGGEAEVLPQPSLLDKARTTV"
     gene            complement(2526..3305)
                     /locus_tag="VN968_04745"
     CDS             complement(2526..3305)
                     /locus_tag="VN968_04745"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HXL28581.1"
                     /translation="MTTPQRFERIAFIASAGLEAQQALKQLCELYGNHEPADADIVVA
                     LGGDGLMLQTLHQHMRSGKPIYGMHRGTVGFLMNEFSSHDLHTRLAAARESVIHPLLM
                     RATDVHGAVHLHHAINEVALFRQTHQAARLRILIDERERMSELIADGIMVATPAGSTA
                     YNLSAQGPILPINAALLALTPICAFRPRRWRGALLPNTAFVVIEVLEGDKRPVAAVAD
                     HDEARDVRRVEVLSDKTISMRMLFDPGHSLEERILSEQFGY"
     gene            3591..5171
                     /locus_tag="VN968_04750"
     CDS             3591..5171
                     /locus_tag="VN968_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HXL28582.1"
                     /translation="MAIAFTSPRISGFALALLAFTMMAGARGQDAPAPPAQQPAATPQ
                     GQKSAGAAGRAAAPTSPGSPAAAEPHRLPPDSTTNQTLALPGRTLNFAATAGSIRLFD
                     DKGEPQADIAYTAYQLDGAEPRSRPVTFLFNGGPGAASAYLQFGNAGPWRLPIGGDAA
                     VSSAPPDLLPNAETWLDFTDLVFIDPVDTGYSRFVATGEDVRKRFFSIDGDVNAIALT
                     IRRWLEKNDRLLSPKFVAGESYGGIRGPKIVHELQAEQGVGVRGLILVSPVMDFREYS
                     GSSILQYVASLPTMAAVAREAKGAVTRADMADVEGYARGDFLSDLVKGTADTSATTRL
                     ADRVAALTGIDQALSRRLAGRFDVAEFRREFDRRDGKVTGRYDASVLGFDPNPDSSFH
                     HFDDPSGDPLIAPLTSAAVEFTTRKLNWRPDGSYQLLNGAVEKAWDFGRGRAPAESIS
                     QLRRILALDPKLKLLVGHGLFDLATPYFGSKILLDQLPAYASPERVKLVVYPGGHMFY
                     SRDASRQAFRAEAEALMK"
     gene            complement(5244..5795)
                     /locus_tag="VN968_04755"
     CDS             complement(5244..5795)
                     /locus_tag="VN968_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL28583.1"
                     /translation="MSKFAIRLLTLAMFSMAVLAAPFATRAYAAPDTDAPPPPPATKS
                     KAKKKTSENQPGIEDAAFSRGYRAAYATIYDRNDYAAAIEQLKALGHDDSAAVANLIG
                     YSYRKLGDYKVSQIWYERALKADPDHVKTWQYYGLWQVEQGNRDQAQYHLSRIAALAG
                     TDSEEYRSLSAALEKPPGTGLIY"
     gene            complement(5869..6411)
                     /locus_tag="VN968_04760"
     CDS             complement(5869..6411)
                     /locus_tag="VN968_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL28584.1"
                     /translation="MNNFAIRLLTLAMYAMALIAVPLATAYAAGSDNPAPPATDTKKE
                     KKKHDKNSSIDDPKFLDGYHKAYATIYDRNDYGSAIEQLKALGQDDRADVANLIGYSY
                     RKLGDYKVSQIWYERALKADPDHVKTWQYYGLWQVEQGNRDQAQYHLNKIAALTGTNS
                     EEYRSLAAALEKPPGTGLVY"
     gene            6713..7039
                     /locus_tag="VN968_04765"
     CDS             6713..7039
                     /locus_tag="VN968_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28585.1"
                     /translation="MLKTISAALLAVSVLAVPALAAGPAKTTQAPTVKSEQAKSAPVK
                     SEQVKTKTTEQAKTGVLNANAKMGRHHHKHHLSHKHMGALKTHKFSKVSKASIKHVAP
                     AAIKRG"
     gene            7261..7683
                     /locus_tag="VN968_04770"
     CDS             7261..7683
                     /locus_tag="VN968_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28586.1"
                     /translation="MGISAKRAAILLLPIALAACAGSDEARNIAYTDDRGVANQPYPN
                     NYRGELLAFMKTYLNNPVGVHDAAMAEPVQRTVGGRLRYLSCLRFTPRESDGSYREVR
                     ERAILYIDGRLDRMIENASESCAGAVYAPFPELEKMTR"
BASE COUNT         1445 a         2394 c         2495 g         1501 t
ORIGIN      
        1 cggccgctac atcgccttct acaacgcaag gcgacctcac gcagcccttg acggccgcac
       61 cccggatcaa gcctactctc acccgctgcc gctccgcacg gcagcataac ccccggccga
      121 cgatccactt aacgacgcgg aaattttgtt caaaggaccg gggccacttc attcgacctt
      181 gacgacgaca taccattcta aggtggggct cgattggatc ttcgccatag attagcgcgc
      241 ggatgggtgg agcgaagcga tacccatcaa ttgcatttcg tggaggtgat gggtttcgcg
      301 aggctcaacc gatgctgcgg ttttgacaac aagaccggct caagtgattt gcccgtcggg
      361 cggtttgtag aggggcgtgt cgagtccctt ttgcaaaaat atttcgcttc acccgtcggg
      421 caaatcatct ctacaaattc gcgccgtccc accccacaac aggggcgtat cgcgatcgtc
      481 acggacgcgg gatgggatgc ggtggacgcg gcagcgtcag gtgtgaaacg tgatgcaggg
      541 cgggtccgac aaggatccgt gagcgatcaa acggcgcgct gacgaacgat gttgttgcgt
      601 acggcgaagt cgtgtggtcc tgacgccccg atgctggcgt caagttcgcg gatgttatgt
      661 cggccctacc gggctcaggc acaacgttag tccgtggacg acggtggcaa gaaagcccgg
      721 tcaccgggga gagcgcgaag gaaaccgtta aaaccattgc gtgcgggaac gccgggtgat
      781 tccggggtac tcgtatactc gtgtgctttt taccaatgca aagcgcacac gaggccgcgg
      841 gtgcaacggg catccggcgt tccccacgcc ctctgagggg gcgaaacgac catgcacgac
      901 tcgggcgcat cgcgccgcga gcgcgaaggc gtatgtggac ttgacgtcat tgcgagcgaa
      961 gcgacccaga ctccgtcatt ccggggcgat gtgaagcatc gaacccggaa tctcgagatt
     1021 ccccgatgcg caattgcgca tctgaggtct ggtcctacgg accatcccgg aatgacggct
     1081 atgggttacg cgctgtttga aaattgaatc gagaacctgc acaggcggca gcgcgctctc
     1141 cccccttgcg ggcccccacg gggggaggga atagaaacgc ggcggctgtc tgaaaattga
     1201 atcgatcgca attcatgaac gcgaacgcgc cacgacctca atcccccggc gcgatgctcg
     1261 gggcgagaat ggcttcgagg tgttcggggc ggtcgcggtt ggcgtgcatc aggaattcgt
     1321 cggcgatgcc gcgcaattgg cggcttagtt cattcgcggt cgagactgtg tcgagcctgg
     1381 tgcgctcgcg cacgatggcc tggatggcgt tgatatgatg ggcgatcagg tcggacggca
     1441 cctcgtcaag attgggcgcg tgttcgatga tccggcacag gctctcggcg gcggccgccg
     1501 ccgaaggaaa accgaacgtg gcggcgtcgc ccttgatgtc atgggcggcg cgaaacagct
     1561 cttcgcggga aaaatccgta aaaccggttt tgagaatcgc ggcgtgggcc gccgccagcc
     1621 ggtcggcctc gaccgacatc cagttcttga attcgccgga caggccggcg agggcctttt
     1681 ccgcccgcgc aatcggatcg tcgagatcct tgtcggcgac ccgccgcagc accttgcgca
     1741 gcggattggg ctgcgtgatc accttgtggt cggcgaagga tttgacctgt atcgtcccgg
     1801 gcttgtcttt ggtcatcgtg gtttcctcgg tcgcgcgcga ctcaaacagt cgtccgctac
     1861 acagtcgttc tggccttgtc gagcagcgac ggctgcggaa ggacttccgc ttcgccgccg
     1921 atgcggcgct cggggccgat ataggcgttg gcggtattgc gccggcggtc cgggccgaaa
     1981 taggttttgg tcttgatgaa agggcgggga ttggcgacca cgttgagaat gcgctgatag
     2041 agccccttgg ccgagatcgg cttggccaaa aactcggtca cgccggcgtc gcgcgcgacc
     2101 gtgacgcggc gcttttcgga atggccggtc agcatgatga tgggggcgta gggattgccc
     2161 ttggattccg gctggcggat catctgggca agctcgaggc cgtcgaaaat cggcatcgac
     2221 cagtcggtga tgacgatatc gggaacgtag tggctgtaca tttcgagcgc ggtggcgccg
     2281 tcctcggatt catagacctc gcgcgcgccg aacgaatgca gcagcgtccg caaaatgcgg
     2341 cgcatatgcg gattgtcgtc gcagacgaga aatcgcagct tgttgaaatc gatgcgaaac
     2401 atggcccggc cccgagggca tacctttgag acggtagacc tgtgttaacc atatcgcggt
     2461 gcgggttaac gaatggttgc gattttgttg tcggcttagc ccgtgatcga cggcgaggcg
     2521 caaaatcagt agccgaactg ctcgctcagg atgcgctctt ccaggctgtg gccgggatcg
     2581 aacagcatcc gcatcgagat ggtcttgtcg gagagcacct cgacgcggcg gacatcgcgc
     2641 gcctcgtcgt ggtcggcgac ggcggcgacc ggccgcttgt cgccctccag cacttcgatc
     2701 accacaaagg cggtgttcgg cagcagggcg ccgcgccagc gccgcggccg gaacgcgcag
     2761 atcggggtca gcgccaaaag cgccgcgttg atcgggagga tcggtccctg cgccgacagg
     2821 ttataggcgg tggatcccgc tggcgtcgcc accatgatgc cgtcggcgat caattcggac
     2881 atccgctcgc gctcgtcgat caggatccgc agccgcgccg cctgatgggt ctggcgaaac
     2941 agcgcgacct cgttgatggc atgatgcaga tgcaccgcgc catgcacatc ggtggcgcgc
     3001 atcaaaagcg gatgaatgac ggattcccgc gccgcggcca gacgcgtatg cagatcgtgc
     3061 gagctgaact cgttcatcag aaagccgacg gtgccgcgat gcatgccgta gatcggtttg
     3121 cccgagcgca tgtgctggtg cagcgtctgc agcatcaagc cgtcgccgcc gagcgccacc
     3181 acgatgtcgg catcggcggg ttcgtgattg ccgtagagtt cgcagagttg cttgagcgcc
     3241 tgctgggcct ccaggccggc gctcgcaatg aaggcaatcc gttcaaatcg ctggggcgtg
     3301 gtcatgggga ttgcggcctc acgttggcgc ggaaagggaa accggtggcg ccgatggtcg
     3361 tctatacgac cttggccggg cttgtcgaga taggccgatt agcgggtgaa ggcaggggac
     3421 ggcaagctgg tgaccatgcc aacttttagt gaaccatgcc aacttgtcgt aattttgcgc
     3481 tagaacggcg cctcgatcca gaaaatgccg cgcataggat taatgtgccg tgccgggtcg
     3541 attgcaggga agcgtggaga acaacgcggc aagcgtggag aacaacggat atggcgatcg
     3601 ctttcacctc gccgcgcatt tcgggcttcg cgctggcgct gctcgcgttc actatgatgg
     3661 ccggcgcgcg cgggcaggat gcgcctgcgc cgcccgcgca gcaacccgca gcgacgcctc
     3721 agggccagaa atcagccggc gcggccggac gcgccgcggc gccaacttcg ccaggctcgc
     3781 cggccgcagc cgaaccacat cgcctgccgc cggattcgac caccaaccag acgctggcgc
     3841 tgccgggccg cacgctcaac tttgccgcga ccgcgggctc gatccgcctg ttcgacgaca
     3901 agggcgagcc gcaagccgat atcgcctaca ccgcctacca gctcgatggc gcggagccgc
     3961 gcagtcggcc ggtgacgttc cttttcaatg gcgggccggg ggctgcttcc gcctacctgc
     4021 aattcggcaa tgccggaccc tggcggctgc ccatcggtgg cgatgccgcg gtctcctccg
     4081 cgccgcccga tctgctgccc aacgccgaga cctggctcga ctttaccgat ctcgtcttca
     4141 tcgatccggt cgacacaggt tacagccgct tcgtcgcgac cggcgaggac gtgcgcaagc
     4201 ggtttttctc gatcgacggc gacgtcaacg cgatcgcgct gacgatccgc cgctggcttg
     4261 aaaagaacga ccgcttgctg tcgcccaaat tcgtcgccgg cgagagctat ggcggcatcc
     4321 gcgggccgaa gatcgttcac gaattgcagg ccgagcaggg cgtcggggtg agaggtctca
     4381 tcctcgtctc tccggtgatg gatttccgcg aatattcagg ctccagcatc ctgcaatatg
     4441 tcgcgagcct tcccacgatg gcggctgtcg cgcgcgaggc caaaggtgct gtgacccgcg
     4501 ccgacatggc cgatgtcgaa ggttacgcgc ggggcgattt tttgagcgat ctcgtcaagg
     4561 gcacagccga cacctcggcc accacgcggc ttgccgaccg ggtggcggcg ctgaccggga
     4621 tcgatcaggc gctgagccgc aggctggccg gacgcttcga cgttgccgaa ttccggcgcg
     4681 aattcgaccg gcgcgacggc aaggtgacgg ggcgttacga cgcctcggtg ctgggtttcg
     4741 atcccaaccc ggattcgagt tttcatcatt tcgacgatcc ctccggcgat cccctgatcg
     4801 cgccgttgac cagtgctgcg gtcgaattca ccacgcgcaa gctcaattgg cggccggacg
     4861 gatcctacca gctgctcaac ggtgcggtcg agaaggcctg ggatttcggc cgtggccgcg
     4921 ctcccgccga gtcgatttcc cagctgcggc ggattctcgc cctcgacccg aagctgaaat
     4981 tgctggtagg gcacggcctg ttcgatctcg ccacgcccta tttcggctca aagatccttc
     5041 tcgatcagct gccggcctat gcttcgcccg agcgcgtcaa gctcgtggtc tatccgggcg
     5101 ggcacatgtt ctattcgcgc gacgcctcgc gccaggcttt ccgggccgag gccgaggcgc
     5161 tgatgaagtg aagccgaagc cgaggcaaaa acaaaaccgg cgcaaccttc aaaggctgcg
     5221 ccggctaatg tgtgtcgtcg aactcagtaa atcaggccgg tgccgggcgg cttttccagt
     5281 gccgcggaca gcgagcgata ttcctcgctg tcggtgccgg ccagcgcggc gatccggctc
     5341 aagtgatatt gcgcctgatc gcggttaccc tgttcgacct gccacagccc gtaatactgc
     5401 caagtcttga cgtggtccgg gtcggctttc agcgcgcgct cgtaccagat ctgcgagacc
     5461 ttgtagtcgc cgagcttgcg gtaggagtag ccgatcaggt tggcgacggc ggcgctgtcg
     5521 tcgtggccga gcgccttgag ctgttcgatc gcggcggcat aatcgttgcg gtcatagatc
     5581 gtggcatagg ccgcgcgata acccctggag aatgccgcat cctcgatgcc gggctggttt
     5641 tcgctggtct tcttcttggc cttggacttg gttgcgggcg gcggcggcgg cgcgtcggtg
     5701 tcgggcgcgg catacgctct ggtggcaaag ggggcggcga gcaccgccat cgagaacatt
     5761 gccagcgtca aaagcctgat cgcaaatttg ctcatgtatc ctcctgttgg cggtgtgtag
     5821 atcaggtttc ctgacttcgc gaagacagga aacccggttt ggtgaagatc agtaaacgag
     5881 accggtgccc ggcggctttt cgagagctgc ggccagcgag cggtattcct cgctgttggt
     5941 gccggtcaag gcagcaatct tgttcaggtg atactgcgcc tggtcgcgat tgccctgctc
     6001 aacctgccac aagccgtaat actgccaggt cttgacgtgg tccgggtctg ccttcagcgc
     6061 gcgctcatac cagatctgcg acaccttgta gtcgccgagc ttgcgatagg agtagccgat
     6121 cagattggcg acgtcggcgc ggtcgtcctg gccgagcgcc ttcaactgct cgatcgccga
     6181 gccatagtcg ttgcggtcgt agatcgtggc ataggccttg tgataaccgt caaggaactt
     6241 cggatcgtcg atgctggagt tcttgtcgtg cttcttcttt tccttcttgg tgtctgtcgc
     6301 cggcggagct ggattatcgc tgcccgcagc ataggccgtc gccagaggta ctgcgatcaa
     6361 cgccatcgca tacatcgcga gcgtcaaaag cctgatcgca aagttgttca tgcatttctc
     6421 ctgattccag ttgggtaatc ttacaccgaa gcccgctgac ttgaacaccc gcgggctttt
     6481 aacattcccg ggcaatcatc ccaatttgag ggaatttttg tgaaccccgt gcggctgtgc
     6541 gctgtccgga acatgaccgg gaacatgacc aagatgtcat gcagatgaac gaagcccgtc
     6601 attgcgcttc agaatgggtt cagcgcgcgg cacgtaacgt cgcccgcatg atcaatgagc
     6661 ccggccaacc cgaccggcga cctcgtgatc cccgttgaaa gaggagacaa ccatgttgaa
     6721 gaccatttcc gccgcgctgc ttgcagtttc cgttctcgcc gttcccgcgc tggccgctgg
     6781 ccccgccaag accacgcaag cgccgaccgt caagagcgag caggccaaga gcgcaccggt
     6841 gaagagcgaa caggtgaaga cgaagaccac tgaacaggcg aagaccggcg tgctgaacgc
     6901 caacgccaag atgggccgcc atcaccacaa gcatcacctc tcgcacaagc acatgggcgc
     6961 gctcaagacc cacaagttct cgaaggtctc gaaggcctcg atcaagcacg tcgcgcctgc
     7021 cgccatcaag cgcggctgaa actgatccgc gggcgtggcc taaccacatt gccccccgcc
     7081 actccctgcg gataacaggc ccggtctgcc gccattgctt cgcaatgacg gcggaccggg
     7141 gcggtttctc ggcaagccaa gaggtcctcg atcatcgaga tgcgaattcc ttttcgcggg
     7201 caaacggtct agaacctccc gggcgtcaga tctttgaata acggatcgca cttgttgcac
     7261 ttggggatat cagcaaagcg cgcggcgatc ctgctgctgc cgatcgcgct cgcggcttgc
     7321 gcgggcagcg atgaggccag aaacatcgcc tataccgacg accgcggcgt cgccaaccag
     7381 ccatatccca acaactaccg cggcgaattg ctggcgttca tgaaaaccta tctcaacaat
     7441 cccgttggcg tgcatgatgc cgcgatggcc gagccggtgc agcgtaccgt cggcgggcgg
     7501 ctgcgttacc tcagttgcct gcgctttacg ccgcgcgaat ccgacggttc ttaccgcgag
     7561 gtgcgcgaac gcgccattct ctatatcgac ggccgcctcg accggatgat cgagaacgcc
     7621 agcgagtcct gcgccggcgc ggtctatgcg ccgttcccgg agctggagaa gatgacgcgg
     7681 tagggcggtc tactctacat gaattcttcc gcgatcgcat caaagccgcg gcgccagccc
     7741 cgcgcgcgat ccggatcgag tggcgttgct tccgaagcca tcctcacgat cacgagttcc
     7801 gcctgcggat ggatgtagat gaattgcccg tggat
//
LOCUS       DATNOI010000131        17344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2440844,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000131 DATNOI010000000
VERSION     DATNOI010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17344
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2440844"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /locus_tag="VN968_04775"
     CDS             <1..1645
                     /locus_tag="VN968_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HXL28587.1"
                     /translation="DIDGVVHEGRNTLMDRWKSVYAQKTDKRKLSEVIGGADIFLGLS
                     APNVLTPDMVKAMADKPLVMALANPTPEIMPDEARKARPDAMICTGRSDFPNQVNNVL
                     CFPFIFRGALDVGATAINEDMKHAAVDAIAQLARDPPVDAIAPGFDSGEAQGFGPGSL
                     IPSPFDPRLILRIAPAVARAAMTSGVATRPIANFEDYTAQLERFAFRSGLVMKPMFAK
                     AKTQPVRVIYAEGEDERVLHATQVVLEENLARPILVGRPSVVEARIKRFGLAIKAGQD
                     FDLINPEDDPRYRSYVQSYIDVAGRHGVTPDAARTVVRTNATVIAALAVVRGEADAMI
                     CGVEGRYMSHLRHVREIVGFLPGVSDFAALALMITSKGAYFIADTQVRPNPSAEELAE
                     MAALAALHVQRFNLKPRVAFVSHSDFGSYDTDSSRKMRRATALLKQNHPEIEADGEMQ
                     GDTALSEAARKLILPHSKLEGEANILIMPNLDTANVAYQMIKALADALPVGPILIGPS
                     RPAHILTPSVTARGVLNMTAVAAVEAQERAGRRQPTLFG"
     gene            2152..2604
                     /locus_tag="VN968_04780"
     CDS             2152..2604
                     /locus_tag="VN968_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXL28588.1"
                     /translation="MPAFIVLGRVLFAVLFIYSGAAKLIGIQATTEFIASKVAIPAML
                     VPYTSQLETMTGMSTPQTLAIVVGAFEVISGLMIALNIGARFFAILLIFFVAATIFYF
                     HDFWNQAPPDNAATLIEALKNLSIIGALFMIVGIGRLSRKAEPAYDDV"
     gene            complement(2614..3123)
                     /locus_tag="VN968_04785"
     CDS             complement(2614..3123)
                     /locus_tag="VN968_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HXL28589.1"
                     /translation="MLADAAHVMPDELKFAGDKRFFAAALDRADLIVHGRRSYEDQPN
                     SPLRKRIILTRSIGGVATDPSNPRATLWNPAAASFEAACDAAGVRSGTVAIIGGPDVF
                     GMFMDRYDSFWLSQAPRVRLPGGEPCFPGVPARSPEAILAAHGLKPGDAEILDRANDV
                     TVTPWRRMA"
     gene            complement(3188..4129)
                     /locus_tag="VN968_04790"
     CDS             complement(3188..4129)
                     /locus_tag="VN968_04790"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXL28590.1"
                     /translation="MRIAAGLIFAGVVSLCASGAAWSQTPATKGAAAPQAAPAPFPAA
                     APAPAKMPCANPNALGIARVVEIDTTGGPGFGFEHFKQLDFLRDKEVVLTFDDGPWPL
                     NTPSVLKTLADECTTGIFFSIGKHATYYPEILKQVLAAGHTVGSHTWSHATLTNKKLT
                     EQQRIEEIEKGFSAVKWALGGASPSPFFRFPALQHPPEMVTYLGTRNIAMFSCDLDSF
                     DFKASSPKQVIDVTMKKLDKLGKGIILMHDFHKHTAEALPELLRKLKDGGYKVVQMKA
                     KAPVQTLAQYDDEILKDAKLPTVSSRPVSSVVQTISE"
     gene            complement(4300..4875)
                     /locus_tag="VN968_04795"
     CDS             complement(4300..4875)
                     /locus_tag="VN968_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HXL28591.1"
                     /translation="MMDRRTMITAALAAIAGSTSTRTVLAEPAMSRITAYAFSFPALA
                     GGDIRLAEYAGRPLLVVNTASLCGYTPQYGGLQELWSRFHDRGLMMIGVPSNDFGGQE
                     PGGATEIAETAQHQYGVTFPIAAKVVVKGPNAHPFYRWAAEARPNDVPRWNFHKYLVG
                     RDGYIAEAFPATIEPSDTRVVTAIGRALAET"
     gene            complement(4946..6595)
                     /locus_tag="VN968_04800"
     CDS             complement(4946..6595)
                     /locus_tag="VN968_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28592.1"
                     /translation="MNQSSTPASTTAPQIDAAKDAFDRLTLLGWRWGLAAIVGGLAAS
                     FFLFGYALIYWRNADMDFMVIYNALVLNDGKPQLFFDHTGYLTILSVKFWFQLLHALG
                     LLDAYSLATMPSAANTAAFDAAMTGAVRAGRVLAWLIATGCVLIFADLVRRVVRDWRV
                     AMLATLAIAFSGGVAVHSRILRSELVAACPVIFALLLLIVVGRRAGVARPLAMAAAAA
                     LCVLGLENKVQAILLIGALPLLILPFGAATSASVAFWNDARSSWLATVIAAIAAIAAA
                     WAVWPLIGTGFDRALLDTAHFHPLLLGRFGIYQAALLVLIGGCMTAYAAIWRVSAAET
                     LASIFAMAAGASIALLALDLEYSTSNVIAVFNPLEKMLTFADASTTDVANGSNLFGMM
                     LLLLDGVASVLARYSFVLHSSPRPTVFLTWLIVPGIILAWRRGEGHAAIQTLALLLAA
                     IGIDALGVRRGLKSEYFIFTDPLIILAGAILLDSLGDLRFHKWAYPLAVALFGLHIAV
                     GQAEPIKYAFMRRGPESICEWNQVYMPLLPLPWCPLPPVRP"
     gene            complement(6759..8420)
                     /locus_tag="VN968_04805"
     CDS             complement(6759..8420)
                     /locus_tag="VN968_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXL28593.1"
                     /translation="MVRVREYVATRRLWRIAVLTAAAAVGAASHAETALYYWSDSDPG
                     FSQPGPPVPPRRQHARRRQAKKVEASLKQTAKPQGPLIIAISIQKQRLKIYDAAGVFA
                     ESPISSGMPGHPTPMGVFSVIQKQKLHHSNIYSGAPMPYMQRITWSGIAIHAGVLPGY
                     PASHGCIRMPMAFAMKMWNWTKMGARVVVTPGEMTPADFSHPLLVAQKVVPQPGVADE
                     PKPDAPAATKTNKASDANGAVKSTISEARPELRPTVGHDENLKPVMGESSAATPLHDR
                     TRTADASNGLPATNAPVTMTDATSSAGNASPRDEAARTDNAPPNAAAVKADDSAEPSI
                     AKSSGSASSDDKPVKTKPPAAAAVTEATKTQDGKTEVKADDARTVASKAKDQTRLDAP
                     KLAPAMAAAPKRSGQISVFVSRKDSKLYARQNFAPLFDVPVTIASSDRPLGTHVFTAQ
                     VDKDDANVLHWSVVSLPVRARYAEQRDEDERGSRRRRVAGAAEIKPMPAADSPAEALD
                     RLTIPAEAMARIAEALSTGGSIIVSDQGIAAGETGEGTDFIVSLR"
     gene            8626..9171
                     /locus_tag="VN968_04810"
     CDS             8626..9171
                     /locus_tag="VN968_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HXL28594.1"
                     /translation="MTSQAASPYDTKYLPLVLFLLAWVLWPGSAFAADEPDLIFRRST
                     VFKWISPNDKLATYGVDDPEVEGVACHFTVPEKGGFKGWLGLAEEVSDISLACRQIGP
                     IHFKGKMEQGDDMFRLRRSLFFKKMQIVRGCDAKRNVLVYMVYSDKLIEGSPKNSTSS
                     VPIMPWGAADAAVQKCGEFVQ"
     gene            10070..10879
                     /locus_tag="VN968_04815"
     CDS             10070..10879
                     /locus_tag="VN968_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL28595.1"
                     /translation="MKHRMGDEESGSDKNGFEAFRQSYQKVSTERSQPTCLVVEDDRT
                     MRHLVMNYLEEHGIRTISASGREEVAGLLAREEPSLVILDLRLGQEDGLDLLREIRSH
                     SDIPVIITTGHRRDEIDRVVGLELGADDYITKPFGLRELLARIRAVLRRREAGQAAVQ
                     RDSERGRCRFGGWQLDRRNRRLSDASGIPVALTKGEYALLIAFLDAPQRPLSREHLLQ
                     ATRIHEDVFDRSIDVQVLRLRRKLETDPNAPRVILTERGVGYVFALPVEPL"
     gene            11311..11601
                     /locus_tag="VN968_04820"
     CDS             11311..11601
                     /locus_tag="VN968_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28596.1"
                     /translation="MARVVGGLAAWGETAFPRLLFAVMSWTMAQALAGCIAYAEAMYS
                     VDLSDPIDGRHPAGPQPEREGRDQLRSHRMLRDLGITRRDIEDFARRGDVCE"
     gene            11670..12641
                     /locus_tag="VN968_04825"
     CDS             11670..12641
                     /locus_tag="VN968_04825"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HXL28597.1"
                     /translation="MPAILRARRRSLLWIVLALLPLAGGAVGQAPDHQASDARPLADQ
                     EPITPVPPPPAADPQKLALGERLFGDPRLSGDGKLACTSCHDLKTNGASDGRTITAHD
                     GSKLPFNTLTVFNAALSFRLNWEGNFRALGAQAESSLENPANMRTSVDEVLGKLNADP
                     LMVQQFRAAYGRAPDRASFLDALVTFERSLLTPGSRFDRWLGGDASALSTEELDGYQL
                     FKSLGCSSCHQGMNIGGNLFERQGIFRPLVPGKPEIVRVPSLRNVATTPPYFHDGSAA
                     TLKEAVHRMAAAQLDRTLSDQQVDCIVAFLGTLTGTYRGAAVVGAPQ"
     gene            12638..15151
                     /locus_tag="VN968_04830"
     CDS             12638..15151
                     /locus_tag="VN968_04830"
                     /inference="COORDINATES: protein motif:HMM:NF010411.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system VirA-like sensor kinase"
                     /protein_id="HXL28598.1"
                     /translation="MRVTPAAAMIPLLVVLLTWLSVCAINPDAELFDRALGEIDRFAM
                     TENALYRDVFTARAGILRNYDPLVREINELRGSLDRLRETAAVDSETTMAVDQMAASI
                     GRQEALVEQFKSDNALLHNSLSFFGRFSVRLASSDLGPAISAAAAAMLHLTLDTSSAA
                     AREVEERLDEVARQARSSGDADSVRALLAHGRLLHDLLPAVDNALKAMRAVPRKRDQD
                     ALRAMVLTRQAASRTTARQFRQLLYATSLLLVAVLVYFGLRLRARAKALQRRAAFEHV
                     IAGISMRFINAQPQNVGIEIEQALADMAGCIGCDRAYFVLSGPAPRMHLWCKAGTSFS
                     PGWPENAPALAARLGPTVDGIIHIPRVKRMPPGENKDACIALGLGGWGCVTYVDNDGT
                     RVALGFDAIGRPCRITAAGELSLLRMALDTILSAVGRHAMEKERARLETRLQQARRME
                     TVGTFTSGMAHNFNNILGGILGHSEVIEEHFGSDARLLRNLGAIRRGAERARDLVDQI
                     LTFGRRRDARRRPLSARALVTEAESLLNVSLPPGIDLAIHEPPAAAVVSGELAQLQQV
                     ILNLCNNAAQAMENAGRIEVETEVHEVTDERSLSHGELQPGRYVRISVRDAGRGMEGA
                     TLARIFEPFFTTRSAGNGLGLATVREIVREHGGAINVQSAPGEGSRFEVWLPGLATAG
                     PASEADGPVLPLGCGETVLMVASDGARLLRDEETLAALGYEPVGFTGADAALAACRAT
                     PERFDAMVVGDFGSAICSLELAAALHAAAPRLPIVLATKSIEEIGADTLVAAGIADVV
                     RWPIAAQEIATALNHCSALRKLEAGAPLSSARGAHSRMH"
     gene            15294..15692
                     /locus_tag="VN968_04835"
     CDS             15294..15692
                     /locus_tag="VN968_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28599.1"
                     /translation="MPQPFPLPGGLPLWFDQQHSTGIIDIFRELFRRARATKQFSRGM
                     KPICALAQTSGNSPWLTNRQRKRRAAPASKTGDGQMRTLSFMLALVFVMAGASIAGSS
                     DTSLPGIGTFAYSGSPVAASAPETIFVAAR"
     gene            15735..16100
                     /locus_tag="VN968_04840"
     CDS             15735..16100
                     /locus_tag="VN968_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28600.1"
                     /translation="MFVRISAVAIIVCVILGSSAQAQVRSPSKLPDRRAEFVRLCAPH
                     MEGRWAHPEAVCGCLHDYAAAVVEDTDLREALLRGISETGVPTIETEWVPRSKRSEIG
                     ATFTKIAKPTLQCMFEPKN"
     gene            complement(16191..16451)
                     /locus_tag="VN968_04845"
     CDS             complement(16191..16451)
                     /locus_tag="VN968_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6719 family protein"
                     /protein_id="HXL28601.1"
                     /translation="MKASHIKCFSLLLLGGLALRAPPASAMADQVSREQDIVGLRLGQ
                     HIQVDDGSCPAGQIKEVSGAKMTPTGILAATRCIPRLGIKRK"
     gene            complement(16472..16624)
                     /locus_tag="VN968_04850"
     CDS             complement(16472..16624)
                     /locus_tag="VN968_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28602.1"
                     /translation="MPGNRNILYLIIGALIVAVGVLGYNLYQTKKEPEGLQINVGPNG
                     LKIQNK"
     gene            16779..>17344
                     /locus_tag="VN968_04855"
     CDS             16779..>17344
                     /locus_tag="VN968_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXL28603.1"
                     /translation="MNAIDTRQRILDAVDAGFDAQLATTRDFVAIPSTRGAEGPCQDM
                     IGDLLRQRGYEVDDWHIDVDDLKDLRGFGPIEHDFSKARTVVGTYRPSSNAGKSLILQ
                     GHCDVVPAGPLDMWQTPPFSPVVKDGRMYGRGACDMKSGTIGALYALDAIRAAGLKPT
                     ARIHFQSVIEEESTGVGALSTLQRGYRA"
BASE COUNT         3189 a         5294 c         5507 g         3354 t
ORIGIN      
        1 cgatatcgac ggcgtggtgc acgaaggccg caacacgctg atggaccgct ggaagtcggt
       61 ctatgcgcag aagaccgaca agcgcaagct ttcagaagtg atcggcggcg ccgacatttt
      121 tctcgggctg tcggctccca acgtgctgac accggacatg gtcaaggcga tggctgacaa
      181 gccattggtg atggcgctcg ccaatccgac gccggagatc atgccggatg aggcccgaaa
      241 agcccgcccc gacgccatga tctgcaccgg gcgctccgac tttcccaatc aggtcaacaa
      301 cgtcttgtgc tttccgttca tctttcgcgg cgcgctcgac gtcggggcca ccgcgatcaa
      361 tgaggatatg aagcacgccg cggtcgacgc aatcgcgcag ctggcgcgcg atccgccggt
      421 ggatgcgatc gcgcccggct tcgacagcgg cgaggcgcaa gggttcggtc cgggctcgct
      481 gattcctagc ccgttcgatc cccgcctgat cctgcgcatc gcgcctgcgg ttgctagagc
      541 ggcaatgacg tcgggcgtcg ccacgcggcc gatcgcgaat ttcgaggact ataccgcgca
      601 actggagcgc tttgcgttcc gctccggcct cgtcatgaag ccgatgttcg ccaaggcgaa
      661 gacccagccc gtccgtgtga tctatgcgga aggcgaggac gaacgcgtgc tgcacgcgac
      721 gcaggtcgtg ctggaggaga atctggcgcg gccaattctg gtcggacgcc cctcggtggt
      781 cgaagcgcgg atcaagcggt tcggtctcgc catcaaggcc ggccaggatt tcgatctcat
      841 caatcccgag gacgatccgc gctatcgatc ctatgtccaa tcctacatcg acgtcgccgg
      901 acggcacggc gtgacgcccg acgccgcgcg cacggtggtg cgcaccaacg cgaccgtcat
      961 cgcggcgctc gcggtggtgc gcggcgaagc tgacgccatg atctgcgggg tcgaaggccg
     1021 ctatatgagc catctgcgcc acgtgcgcga gatcgtcggc ttccttccgg gcgtgagtga
     1081 tttcgctgca ttggcgctga tgatcaccag caagggcgcc tacttcatcg ccgataccca
     1141 ggtgcggccg aacccgagcg ccgaggagct tgcggagatg gcggcgctgg cggcgctcca
     1201 tgtccagcgc ttcaatctca agcccagggt cgcgttcgtg tcgcactcag atttcggcag
     1261 ttacgatacc gactcgtcgc gcaagatgcg ccgggccacc gcacttctga agcaaaacca
     1321 cccggagatc gaagcggacg gtgaaatgca gggcgatacc gcgctgtcgg aagcggcaag
     1381 gaagctgatc ctgccgcact caaaactgga aggcgaggcc aatatcctga tcatgccgaa
     1441 tcttgatacc gccaacgtcg cgtatcagat gatcaaggcg ctggccgatg cgcttccggt
     1501 cgggccgatc ttgatcgggc cctcgcgtcc ggcgcatatt ctcaccccct cggtgaccgc
     1561 acgcggcgtt ctcaacatga cggcggtggc cgcggtcgaa gcccaggaac gcgccggccg
     1621 ccggcaaccg accctgtttg gctaggcttg caggtattcc gacaagcctg cttccgacaa
     1681 gcctgcttcc gacaaggctg ccgagggcgg ataccggtgc ttggcgagca ggttggtgcg
     1741 gattgcgcgg gtctgatctg cggcagaagt agactaatct gcggtcgaat acgcgcaaga
     1801 tcttggtcag gcgccttttg ttgagttaac ccgattcccc gtggcggaaa aatgctctag
     1861 tgtgacagtt tggaagttat cttcattgtt gcagcgcgtc agcgccggag gacttctgag
     1921 caaagccaca ctagcatcat aatttccagt gttctgtcga agccgaagtt cgcaacacaa
     1981 gatgctgcag tgatgcggat ttcgaattct gcacgttagt gaaaatccga tcgagttccc
     2041 gccgcggcga ccgcgcattc gaaacgattt gatagtttgc aacctgaccg gaatgcccat
     2101 aaccgctccc ctgcagcaac cccgcatcag cagcattcaa tgaggccgac catgccagcg
     2161 tttattgttt taggacgcgt tctgttcgcc gtgctcttta tctactcggg agcagccaag
     2221 ttaatcggca ttcaggccac cacggagttt atcgcatcca aggttgcaat tccggccatg
     2281 cttgttccct acacgtcgca gctggagacg atgacgggaa tgtcgacgcc gcaaacgctc
     2341 gccatcgtcg tcggagcctt cgaggtcatc agcgggttga tgatcgcgct caatatcggt
     2401 gcgcggttct ttgctatttt gttgatcttc tttgtggctg cgacaatttt ctatttccac
     2461 gatttctgga atcaggctcc gccggacaat gccgcgaccc tcatcgaggc gctgaagaat
     2521 ctgtcgatca tcggcgcgct gttcatgatc gtcggaattg gccggttgtc gcggaaggcg
     2581 gagcccgcct atgacgatgt ctgagccgga cgtctaagcc atccgccgcc acggggtgac
     2641 ggtgacgtca ttggcgcggt cgaggatctc ggcgtcgccg ggtttcagac cgtgcgccgc
     2701 caggatggct tctggcgagc gcgcagggac tccgggaaag catggctcgc cgcccggcaa
     2761 ccgcacccgg ggcgcctgcg agagccagaa cgagtcgtag cgatccatga acattccgaa
     2821 cacgtccggt ccgccaatga tcgcgacggt gccggaacgc acgccggcgg catcgcaggc
     2881 tgcttcgaac gaagctgcgg ctggattcca cagggtggcc ctggggttgg aagggtcggt
     2941 cgcgaccccg ccgatgctgc gggtcaggat gatccgcttt cgcagcggcg agttcggctg
     3001 gtcctcatag gaccgccttc cgtgaacgat gaggtcggcg cgatcgagcg cggccgcgaa
     3061 gaaccgcttg tcgcccgcga atttcagttc gtcgggcatc acatgcgcgg catccgccag
     3121 catgccgtca gccgacacga tgacatagcc gtcaatgcgc agagctggca ccgcgcgcgg
     3181 gcctggtcta ttccgaaatc gtctgcacca cgctggagac gggcctggaa ctgacggtcg
     3241 gcaatttggc gtctttgagg atttcgtcgt cgtactgcgc cagggtctgc accggggcct
     3301 tggccttcat ctgcacgacc ttgtagccgc cgtccttcag tttgcgcagc agttcgggga
     3361 gggcttccgc ggtgtgcttg tggaaatcat gcatcaggat gatgcctttg ccgagcttgt
     3421 cgagcttctt catcgtgacg tcgattacct gcttcggcga gcttgccttg aaatcgaacg
     3481 agtcgaggtc gcaggagaac atcgcgatgt ttctggtgcc gagataggtc accatttcag
     3541 gcggatgctg cagtgcaggg aagcggaaga atggcgacgg cgaagcgccg cccagcgccc
     3601 atttcaccgc gctgaaacct ttttcgatct cctcgatgcg ctgctgttcg gtcagtttct
     3661 tgttggtcag ggtcgcgtgc gaccaggtgt gagatccgac cgtgtggccg gccgcgagca
     3721 cctgtttcag gatttccgga tagtaggtcg cgtgcttgcc gatcgaaaag aaaatgccgg
     3781 tcgtgcattc gtccgccagg gtcttcagca ccgagggtgt attgagcggc cacgggccgt
     3841 cgtcaaacgt cagcaccact tccttgtcgc gcaggaaatc gagttgcttg aagtgctcga
     3901 aaccgaaccc cggtccgccc gtggtgtcga tttcgacgac gcgcgcgatc ccgagcgcat
     3961 tggggtttgc gcaaggcatt tttgccggag ccggggctgc tgcggggaag ggcgctggag
     4021 cggcttgcgg ggctgccgcg cccttggtgg ccggggtctg tgaccatgcc gcgcctgacg
     4081 cacacagcga aaccacacct gcaaagatca aacctgccgc aatgcgcatc gtatttcccc
     4141 ttatgctgac gcgtccgggc ctcggctttc gccttcgccg gaacggtttc ctgcccctgc
     4201 cgacaatagc ctagtaggac ctgcgccgcc acggcaaagg cttttgttgc cagctgtcat
     4261 tttttccgtc gaacgtgtta attccgggga gaccgcgcgt caggtctccg caagcgcccg
     4321 cccgatggcg gtcacgactc gcgtatccga tggctcgatg gtcgcaggga acgcctcggc
     4381 gatgtagccg tcgcgcccga ccagatattt gtgaaagttc cagcgtggaa catcgttcgg
     4441 ccgtgcctcc gcagcccatc ggtagaacgg atgcgcgttc ggacctttga cgacaacttt
     4501 ggccgcgatc ggaaaggtaa cgccatattg gtgctgtgcg gtctcggcga tttcagttgc
     4561 cccgccgggc tcctggccgc cgaaatcgtt ggagggcacg ccgatcatca tcaagccccg
     4621 gtcgtgaaac cggctccaca attcctgcag tccgccatat tgcggcgtgt aaccgcacaa
     4681 tgacgccgtg ttgacgacga gcagcggacg gccggcatat tcggccagcc tgatatcgcc
     4741 gccggccagc gccggaaacg aaaaggcata ggcggtgatt cggctcatgg cgggttctgc
     4801 cagaacggtc cgtgtcgagg ttgaacctgc gatagcggcc agcgccgcgg tgatcattgt
     4861 cctgcgatcc atcatagcca cctcgggcgc aattcagctg cgaaagaata gtccaacggg
     4921 cgcgtgaccg ctgctcaaca agccgttatg gccggaccgg aggaaggggg caccacggca
     4981 gcggcaacag cggcatataa acctggttcc attcgcatat cgattccggc cccctgcgca
     5041 tgaaggcgta ttttattggc tcggcctggc cgacggcgat atgcagcccg aacagggcca
     5101 cggcgagggg ataggcccat ttgtgaaagc gcagatcccc aagcgaatcg agcaggattg
     5161 cgcctgcgag gatgatcagg ggatcggtga agatgaaata ttcggatttc aatccgcgcc
     5221 gcacgcccaa cgcgtcgatt ccgatcgcgg ccagcagaag cgcaagcgtc tggatcgcgg
     5281 catgcccttc gccgcgccgc cacgccagga ttatgccggg aacgatcagc caggtcagaa
     5341 acaccgtcgg acgcggtgag gagtgcagca cgaagctgta gcgcgccaga accgaagcca
     5401 ccccgtcgag cagaagcaac atcattccga acagattgga tccgttggcg acatccgtgg
     5461 tgctggcatc ggcaaaagtc agcattttct ccagcggatt gaatacggca atcacattgc
     5521 tggtgctgta ttcgagatcg agcgccagca gcgcaatcga cgcgccggcc gccatcgcaa
     5581 atatcgacgc cagcgtctcg gcggcgctga cacgccagat cgcagcatag gcggtcatgc
     5641 atccgccgat caggaccagc aatgcggcct gatagatgcc gaagcgaccc agcaacaagg
     5701 gatggaaatg cgcggtgtcg agcagggcgc ggtcaaaacc ggttccgatc agcggccaca
     5761 cggcccatgc cgccgcaatt gccgcgatgg ccgcgataac agtcgccagc cagcttgatc
     5821 gcgcatcgtt ccagaacgcg acgctggcgc ttgtcgcagc tccgaacggc aggataagca
     5881 gcggcagcgc gccgatcagc aagatcgcct gcaccttgtt ctcaagcccc agcacgcaca
     5941 aggccgccgc agccgccatc gccagcggac gggcaacgcc ggcgcggcgt ccgactacga
     6001 tcagcagcaa cagcgcaaag atgaccggac aggccgcgac cagctcgctg cgcaaaatgc
     6061 gcgagtgcac ggcgacgccg ccggagaagg caatcgcaag cgttgcgagc atggcgacgc
     6121 gccagtcccg caccacgcgg cgaacaaggt ctgcaaaaat cagcacgcaa ccggtcgcga
     6181 tcagccaggc cagaacgcgc ccggcgcgaa cagcgccggt catggcggca tcgaatgccg
     6241 cggtgttggc cgccgacggc atggttgcca gcgaataggc gtcaagcagg ccgagcgcgt
     6301 gcagcagctg gaaccagaac ttgaccgaca ggatggtcag atagccggta tgatcgaaga
     6361 acagctgcgg cttgccgtcg ttgaggacaa gcgcgttgta gatcaccatg aaatccatgt
     6421 cggcgttgcg ccaatagatc aaggcgtagc cgaacagaaa aaacgatgcc gcgaggccgc
     6481 caacgatggc tgccaacccc catcgccagc caagcaacgt cagccggtcg aaggcatcct
     6541 tcgctgcgtc gatctgcggt gcggttgtgg aggccggagt ggaagactgg ttcatgccgg
     6601 cgggtctttt agctcacccg ccggttcaaa gcgacggcgg ctgaaaggcc agggccgtct
     6661 agcgcaggat gacttgtctt cgaatcgtca tcctgctcta tcgttttgtt tgagcatgat
     6721 ctttcggaaa accggtatcc acttttccgg atcatgtttt agcgcagcga aacgatgaaa
     6781 tcagttcctt cgccggtttc gccggccgcg atgccttgat cggagacgat gatcgagccg
     6841 ccggtcgaaa gcgcctcggc aattcgcgcc atcgcttccg ccgggatggt gaggcgatcc
     6901 agcgcttcgg ccgggctgtc cgccgccggc atcggcttga tctcggccgc acccgctacc
     6961 ctgcgccgcc gcgacccgcg ttcgtcttcg tcgcgctgtt cggcgtaccg cgcccgcacc
     7021 ggcagcgaga ccaccgacca atgcaggaca ttggcgtcgt ccttgtcgac ctgggcggtg
     7081 aacacgtggg ttccaagcgg ccggtcgctc gacgcgatcg ttaccggcac gtcgaacagc
     7141 ggcgcgaaat tctgccgcgc atagagtttt gaatccttgc ggctgacgaa caccgagatc
     7201 tggccgctgc gctttggcgc cgctgccatt gccggggcga gttttggggc gtcgaggcga
     7261 gtctgatctt ttgctttcga ggctaccgtc ctggcgtcat ccgccttcac ttcggtcttg
     7321 ccgtcttggg tcttcgtggc ttccgttaca gccgcggctg caggcggttt ggtcttgacc
     7381 ggcttgtcgt cggaggatgc tgaaccgctg ctctttgcga tggacggttc ggctgaatcg
     7441 tcagccttga cggcggcggc gttcggcggc gcgttgtcag ttcgggcggc ctcgtcgcgg
     7501 ggcgacgcat tgccagcgga ggatgtcgca tcggtcatcg tgaccggggc gttggtggcc
     7561 ggcaagccgt tgctggcatc cgcggtgcgg gtccggtcgt gcaacggtgt agccgccgac
     7621 gattcgccca tgaccggctt aaggttctcg tcgtgtccga ccgtcggtct cagttccggg
     7681 cgcgcttccg aaatcgtgga cttcaccgcc ccgttcgcgt ccgacgcctt gttggttttc
     7741 gtggccgcag gcgcatccgg tttcggctcg tcggcgacac ccggctgcgg cacaaccttc
     7801 tgcgcgacca gcagcggatg cgaaaaatca gcgggcgtca tctcgccggg cgtgaccaca
     7861 acccgcgcgc ccatcttggt ccagttccac atcttcatgg caaatgccat cggcatgcgg
     7921 atgcaaccat gcgacgccgg ataaccgggg agcacgcccg cgtggatggc gatgcccgac
     7981 cacgtgatcc gctgcatgta aggcatcggc gcgccgctat agatgttgga gtggtgcagc
     8041 ttctgctttt ggatgacgct gaaaacgccc atcggcgtcg gatgaccggg catgccgctg
     8101 gaaatcggac tttccgcgaa aacaccggcg gcgtcgtaaa tcttcagcct ttgcttttgg
     8161 atcgagatcg cgatgatcag cggtccctgc ggcttggctg tttgcttcag cgacgcctcg
     8221 accttttttg cctgacgacg ccgagcgtgc tgccgccgcg gaggcacagg cggaccgggc
     8281 tgcgaaaatc ctggatcgga atccgaccag taataaagcg cggtttccgc atgcgaggcg
     8341 gcgccgaccg ctgcggctgc cgttaaaacc gcaattcgcc agagccggcg ggtcgcaaca
     8401 tactcacgca cgcgtaccat cctcgaatca cagtctcatg tttgttgtcg cagacgagaa
     8461 acgggttcac cagcacaagg cctcgacccc ccgcgatttt cgcccaaagc gtaccaaaaa
     8521 agcctcacac aatcagttaa cggcgaacag tggcggtcat cgggcagcct tcctgccgaa
     8581 cgtccgcgcc actacatagg tcgaagcccg ccgacggaga acttcatgac atcccaagcc
     8641 gcaagcccgt atgacacgaa gtatttacct ctggttttgt tcctgcttgc ttgggtgctg
     8701 tggccgggtt cggcgttcgc cgccgacgag ccggacttga ttttccggcg ctcgaccgtg
     8761 ttcaaatgga tcagcccgaa cgacaagctt gcgacctatg gcgtcgatga ccccgaggtc
     8821 gagggcgtgg cctgtcattt tacggtgccg gagaagggcg gcttcaaagg ctggctgggg
     8881 ttagcagagg aagtgtccga catttcactg gcctgccgcc agatcggtcc aattcacttc
     8941 aagggcaaga tggagcaggg cgacgatatg tttcggctgc gacgctcgct gttcttcaag
     9001 aagatgcaaa tcgtccgtgg ctgcgacgcc aagcggaacg tgctggtcta tatggtctat
     9061 tcggacaaac tgatcgaggg atcgccgaag aattcaactt cctcggtgcc catcatgccc
     9121 tggggagccg ccgatgccgc ggtgcagaaa tgcggcgaat tcgtccagtg acgggaatct
     9181 gaccgaagga tccgtaaatt atcgcgttac cgggctcccg ctcggccgga cccatggttc
     9241 agctcgcggt ttcggatcgc ggtagcccac cagatgcacg aacttttgcg gagctgattc
     9301 gttgccgcga ccgccatcgc cgcactgctg cgacattgcg ccacaaacct tgaccggctc
     9361 tttctgccgg ggatctggat cgtcgatccg cggcgcgaga gagccgtcgc gccctgtcaa
     9421 acattcaacg atccgctggc cgcctttgcg ggtcggggtt ttcttgccaa catcgatgtt
     9481 gccattgccc acgaccaaaa tccttttgaa tacatcgcaa cacgcggcta ctcgggaaag
     9541 ttgaagatcg ttcgaccgag ccggtcggag cgatgcttcg ggtcacggtc ggcgaatccc
     9601 cgagctgctt ttccaccaga gaaaggttaa gaattcggcc ggtgcgtcga acccgccggt
     9661 cctggccggg cagcgttcgg gcggtgatgc gacatcgccg cacgcccgac gttttccgcg
     9721 cgctgttggt gttacaggtg ttgccccata accgttcgga aatcgtgccg ccctcctcaa
     9781 tgcatccgat cgtcgcttcg atgaccgggt cggtaaatca cggtctccgt cagacagcgc
     9841 agccaatcct ctgtgcccgg aaacagacgg tcgcaggcag caatgtcgtc cactttttgg
     9901 tcgcgaaata gccgcgttca ctgaacggct gacgcgctga acatcgcgtc aatgttaccg
     9961 atgatggaga tcgtcgctgg tattatgctg cagtgcggaa tgtataatgc atacactcga
    10021 ttagctctta gagaaattgc agatgaccct caggaccgca attggtgcga tgaaacaccg
    10081 tatgggcgac gaagaaagcg ggtcggacaa gaacggattc gaagcgtttc gtcagtccta
    10141 ccaaaaagtt agtaccgaac gtagccagcc gacatgcctt gtggtggagg acgaccgcac
    10201 catgcggcat ctggtgatga actatcttga agagcatggc atccggacca tctcggcttc
    10261 cggacgagag gaggtggccg gcttgttagc tcgcgaggag ccgagcctcg tgatcctcga
    10321 cttgcggctc ggccaggagg acggtctcga tctgctccgc gagatccggt cccattcgga
    10381 tattcccgtg atcatcacca cgggtcatcg tcgcgacgaa atcgaccggg tggttggact
    10441 ggagctgggt gcggatgact acatcaccaa gccattcggt ctgagggaat tgctggcgcg
    10501 catccgggcg gtgctgcgcc ggcgggaagc cgggcaggcc gccgtccagc gcgactcgga
    10561 aagaggccgc tgcagatttg gcggctggca gcttgatcgg cgcaaccggc gcctcagtga
    10621 tgccagcgga atcccggtcg cgctgaccaa gggcgaatat gcgctgctga ttgcgtttct
    10681 cgatgcgccg caacgaccgc tgtcccgcga acacctcttg caggcaacac gcatccacga
    10741 agacgtgttc gaccgcagta tcgacgttca ggtgctgcgc ctgcgacgca agcttgagac
    10801 cgatccaaac gcgcctcgtg tcatcttgac cgagcgcggc gtcggctacg tgtttgcact
    10861 gcccgtcgaa ccgctctgac gatgccgccc cgaacagaac ggcacgggct gccggttttc
    10921 ggagccggaa aggtggcgcg ccgcgttaca accgtagcct caatctccgg tgctggaata
    10981 ttgcagcaat gtgcgcaagg cagaatctcc cgcgttagta gttgttggga gaggtcgatg
    11041 cgatatgaaa accggttacc tggcgaccga tcgtcgcgca ggcttatcaa tcagacaggc
    11101 cagacggcaa tatttttgcg atattcggcg gtggaaactg gcacaaggct cgaaggacca
    11161 tccgcagcct cggcgccgcg ggtttggtca tcccgcgaaa ttaaccgacc cccgactcaa
    11221 gattggccca gcggcgacgg cagagcgctc cggacgtcgt cagatgacgc caggtcgcgc
    11281 cgcgcgattt ccgtagcggc ggctgggttg atggcgaggg tcgtcggagg ccttgccgcg
    11341 tggggcgaga ctgcatttcc gcgccttctg ttcgcggtca tgtcctggac gatggcgcag
    11401 gctctggcgg gctgcattgc ctatgccgaa gcgatgtatt ccgtggacct gagcgatccc
    11461 atcgatggcc gccatccggc gggcccgcaa ccggagcgtg aaggtcgcga tcagcttcgg
    11521 agccatcgga tgctgcgaga ccttggaatt actcgccgcg acatcgaaga ctttgcgcgg
    11581 cgcggagacg tttgcgaatg atacgatcgt ggtcgccgac tttgcgtcgg cgataccctt
    11641 cggaaatttc cgtcgcgcgc gggtctgtgg tgccggccat cctgcgcgcg cgccggcggt
    11701 cgctgctgtg gatcgttttg gcgctactgc ccttggcggg cggagcggtt gggcaagctc
    11761 cggatcatca agcctccgac gcaaggccgt tggccgatca ggagccgata acgcccgttc
    11821 cgccgccgcc cgcggccgat ccgcagaagt tggcgcttgg cgagcgtctg ttcggcgatc
    11881 cgcgattgtc cggcgacggg aagcttgcat gcacgtcctg tcacgacctg aagaccaatg
    11941 gtgccagcga tggccgaacc atcacggcgc atgacggatc gaagcttcca ttcaacaccc
    12001 tgacggtctt caacgcggcg ctcagctttc gcttgaactg ggagggcaac tttcgggcgc
    12061 tcggggcgca ggctgagtcg tcgttggaaa acccggccaa catgcggacc agcgttgacg
    12121 aagttctcgg caagttgaac gccgatccgc tgatggtgca gcagtttcgc gcggcctacg
    12181 gtcgggcgcc ggatcgggcg agcttcctgg acgccctggt taccttcgaa cgatccctgc
    12241 tcacgccggg aagccggttc gaccgctggc tcggcggtga tgcgtcggcg ctctcgaccg
    12301 aggaactgga cggctatcaa ttgttcaagt cgctcggctg cagctcctgc catcagggga
    12361 tgaatatcgg aggcaatctg ttcgaacggc aggggatctt ccgtccgctg gtcccgggga
    12421 aacccgagat cgtgcgcgtg cccagcctgc gcaacgtggc gacgacaccg ccctattttc
    12481 atgacggcag cgcagcaacc ctcaaggagg cggtgcacag gatggccgcg gcgcagcttg
    12541 atcggacgtt gtcggatcaa caggtcgatt gcatcgtcgc cttcctcggg acgctgaccg
    12601 gaacctatcg cggtgcggct gtcgtcgggg cgccgcaatg agggttacac cggccgcagc
    12661 gatgataccg cttcttgttg tgctgctcac ctggctgtcg gtgtgcgcga tcaatccgga
    12721 tgcggaactg ttcgaccgtg ccctcgggga gatcgaccgt ttcgcaatga cggaaaacgc
    12781 gctgtaccgc gatgtcttca cggcacgcgc cggcatcttg cgcaactacg atccgctggt
    12841 tcgggagatc aacgaactgc gcggttcgct cgatcggttg cgcgagaccg ccgccgtcga
    12901 ttccgagacc accatggcgg tcgatcaaat ggcggcgtcg atcggcaggc aggaggcgtt
    12961 ggtcgagcag ttcaagagcg acaatgccct gttgcacaac tcgctttcgt tcttcgggcg
    13021 gttcagtgtc cgcctcgcgt catcggacct cggtccggcc atcagcgccg cggccgccgc
    13081 catgctgcat ctgacgctcg atacctcgtc agcggctgcg cgcgaggtcg aggagcggtt
    13141 ggatgaagtc gcccggcagg cgcgctcctc cggtgatgcc gattccgtcc gggcgctgct
    13201 ggcacacgga cgactgcttc atgaccttct ccccgcggtc gacaatgcct tgaaggccat
    13261 gcgcgccgtg ccgcgaaagc gagaccagga tgctctgcgt gccatggtgc tgacgcgcca
    13321 ggccgcatcg cggacaacgg cacggcaatt tcgccagctg ctctacgcaa cctcgctgct
    13381 tctggtcgcc gtcctggtct atttcggcct gcggctgcgg gcccgggcga aagcgcttca
    13441 gcgccgtgcg gcgttcgagc acgtgatcgc gggcatctcg atgcgcttca tcaacgcgca
    13501 gccccaaaat gtcggcatcg agatcgaaca ggcgctcgcc gacatggccg ggtgcatcgg
    13561 ctgcgaccgt gcttatttcg tgctgtctgg tccggcgccg cgaatgcacc tctggtgcaa
    13621 ggcgggaacg agtttttcgc cgggctggcc ggagaacgcg cctgcgcttg cggcgcgact
    13681 gggcccgacg gtcgatggaa ttatccacat tcctcgcgtc aaacggatgc cgccgggcga
    13741 aaacaaggat gcgtgcattg cccttgggct ggggggatgg gggtgcgtca cctatgtcga
    13801 caacgacggc actcgcgtcg cgcttggttt cgatgcgatc ggccggcctt gtcgcatcac
    13861 ggcggctggc gaactcagcc tgttgcgcat ggcgctcgat accattctgt ccgcggtagg
    13921 gcggcacgcg atggaaaagg aacgggcacg gctggagacg cgcctgcagc aggcccgccg
    13981 catggagacg gtaggcacct tcaccagtgg catggcccat aacttcaaca atattttggg
    14041 cgggattctc ggccattcgg aagtgataga agagcacttc ggttcggacg cccggctgct
    14101 tcgcaatctc ggtgcgattc gccgaggcgc ggagcgagcg cgcgatctcg tcgaccagat
    14161 tctgacattc ggacgccgcc gcgatgcgcg tcgtcggccc ctcagtgcgc gggctttggt
    14221 taccgaggcg gagtcgctgc tgaatgtctc gctgccgcca gggatcgatc ttgcgatcca
    14281 tgaaccgccg gcggcggccg tcgtgtctgg cgagcttgcc caactgcagc aggtgatcct
    14341 caatctctgc aacaatgccg cgcaggcgat ggaaaacgcc ggccgcatcg aggtcgagac
    14401 cgaggtgcac gaagtgaccg acgagcgctc cctcagtcat ggcgagcttc aaccgggccg
    14461 ttacgtgcgg atctcggtgc gcgatgccgg ccgcggcatg gagggggcta cgctggcccg
    14521 gatcttcgag ccgtttttca cgacacgttc ggccggcaac ggcctgggat tagcgacggt
    14581 gcgcgagatc gttcgcgagc acggcggagc gatcaacgtg cagagcgcac caggcgaggg
    14641 cagccgtttc gaggtttggc tgcccggcct ggcgaccgcc gggccggcgt cggaggcgga
    14701 cgggccggta ctcccgctgg gatgcggcga aacggtgttg atggtcgcga gcgatggcgc
    14761 gcgattgctg agggacgaag agacgttggc ggcattgggc tacgagccgg tcggattcac
    14821 cggcgccgac gcggcgttgg ccgcatgtcg cgcgacaccc gagcggttcg acgcgatggt
    14881 ggtgggagat ttcggatccg ccatctgctc gctcgaactg gcggcagcgc tgcatgcggc
    14941 agcgccccgc ctgccgatcg tgctggcgac caaatcgatc gaagaaatcg gcgccgatac
    15001 tttggtggcg gcaggcatcg cggacgtcgt gcgttggccg attgctgccc aggagatcgc
    15061 gaccgcactc aatcattgtt cggcgcttag gaaactcgaa gcgggggcgc cgctcagttc
    15121 ggcacgcggc gcccattctc ggatgcattg agccgatcgg cggcgcgacg gcttgagccc
    15181 cgtttcgata gaaccgaaac ggggttctag attcttgatt tgacgcgttt tcttgacgcg
    15241 aaccggtctc cacttcgctt gaaaacgctc tattggttca ccaacgcgca ggcatgccgc
    15301 agccttttcc gcttccgggt ggattaccgc tgtggttcga tcagcagcat tcgactggaa
    15361 tcattgatat tttcagggaa ttgtttcgtc gggcccgggc gacgaaacaa ttctcgcgcg
    15421 ggatgaaacc aatttgcgct ttggcgcaga catccggaaa cagcccttgg ttaacaaacc
    15481 gtcaacgaaa acggcgcgcg gcgccggcgt ccaaaaccgg agacggtcaa atgcgaaccc
    15541 tcagcttcat gcttgcgctc gtcttcgtga tggctggtgc ctcgattgcc ggttcgtcgg
    15601 atacctcctt gcccggcatc ggaacgtttg cgtatagcgg ctcgccggtc gccgcttcag
    15661 cccctgaaac tatttttgtt gcagcccgtt gaggcgcagc tgagttgaac aagaaaatcg
    15721 tcggtaaggc gcccatgttt gtccgcatca gtgctgtcgc aattatcgtc tgcgttattc
    15781 tgggaagctc ggcgcaggcc caggtccgat cgccgtcaaa acttcccgac cggcgcgcgg
    15841 agttcgtgcg gctgtgcgcg cctcacatgg agggacgctg ggcgcacccc gaggccgtct
    15901 gcggctgcct gcacgactat gccgccgccg tcgtcgagga taccgacctt cgcgaagcct
    15961 tgctgcgcgg tatcagcgag accggcgtgc cgaccatcga gaccgagtgg gtgccgcgat
    16021 caaaacgatc cgagatcggc gcaaccttta cgaagatcgc caagccgacg ctgcaatgca
    16081 tgttcgaacc gaagaactga agcgtgacgc ggtctggcgc ggattgatcg gcgaatattt
    16141 cagctggtag gtgatgtcgc tgcgctcgcc gagcagcgat ccggctgcat ctatttccgc
    16201 ttgatcccca accgcggaat acatcttgtc gcagcgagga ttccggtcgg ggtcatcttg
    16261 gcgccggata cttccttgat ttggccggcg gggcaggaac cgtcatcgac ctggatatgc
    16321 tggcccagcc gcaaaccgac gatatcctgt tcgcgcgata cctgatccgc catggccgat
    16381 gccggcggcg cgcgcagcgc gaggccgccg agcagcagaa gtgaaaagca tttgatgtgg
    16441 gaggctttca tgatcgccgc agcgcgccgt ctcacttgtt ctggatcttc aggccgttcg
    16501 ggccgacatt gatctgcagg ccctccggtt ccttcttggt ctggtagaga ttgtagccga
    16561 gcacgcccac cgcgacgatc agcgcgccga taatgagata caagatgttg cggttgccgg
    16621 gcatctgacg tctccgttgc gatcgcgaat tcgcgccatt gtattgatcg gcctgcgatt
    16681 ctcatagcga tctaaaacgt ctgcaaagtc aggcaaagtg tgatttgatc ggtgcgcgcg
    16741 gccttcgcga ggcggcagac aacgggaaca atgacgcaat gaacgctatc gataccaggc
    16801 aaaggatcct cgatgccgtc gacgcaggtt tcgacgcgca gctcgccacc acgcgggatt
    16861 ttgttgcaat tccctcgacc cgcggtgccg aaggtccttg ccaggacatg atcggcgatc
    16921 tcttgcggca gcgcggctac gaggtcgacg actggcatat cgatgtcgac gatctgaaag
    16981 acctgcgtgg cttcggcccg atcgagcatg atttttccaa ggcccgcacc gtggtcggca
    17041 cttaccgtcc ttcaagcaat gccggcaaat cgctgatcct gcagggacat tgcgacgtgg
    17101 tcccggccgg accgctcgac atgtggcaga cgccgccgtt ctcgccggtc gtgaaggacg
    17161 gcaggatgta tggccgcggc gcctgcgata tgaagtccgg caccatcggc gcgctctatg
    17221 cgctcgatgc gatcagggcc gcggggctca agccgaccgc gcggattcac tttcagtcgg
    17281 tcatcgagga ggagagcacc ggcgtcggcg cgctctcgac cctgcagcgc ggctaccgtg
    17341 ccga
//
LOCUS       DATNOI010000132         6833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1899750,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000132 DATNOI010000000
VERSION     DATNOI010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6833
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1899750"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1179
                     /locus_tag="VN968_04860"
     CDS             136..1179
                     /locus_tag="VN968_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXL28604.1"
                     /translation="MSVQNVMVTADIAARRRSGVLAGLLILVVLLALISLGIGPVRLS
                     PVAVIDALFGGGSDVQQVIVREIRLPRTILGFAIGAILGLSGAALQGLLRNPLASPSL
                     FGAPQSAAFGAVLVIALGLADVRSYALPVAAIAMAFASVFVLLTIAGRNAGLLILILA
                     GLAISSLAGAATALVMNLSSNPFVVLEIAFWLLGSLEDRSFRHVVLALPFIVTGAIVL
                     LSQRNAFRALSLGEETAQSLGVDVGRLRLLVITGVALGVGGAVAVTGTIGFIGLVAPH
                     LMRPLIGHDPTRLLVPSAFTGAALLLSADIAVRIIPSTTDIKVGVLTSIIGVPFFLYL
                     IMRERRALGGGVA"
     gene            1176..1958
                     /locus_tag="VN968_04865"
     CDS             1176..1958
                     /locus_tag="VN968_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28605.1"
                     /translation="MTASAFLNAWGVNVTLAGRLVLNDVSLALSSGHLVALVGPNGAG
                     KTTLLRALAGLVPSEGVIEVGGDALSSLALRERARRFAYLPQGHVVHWPLAARDIVAL
                     GRYPHGATDPARLTPKDAEAVLRAMQATDVVEFSERRVTELSGGERSRVALARVLAVE
                     APVILADEPTASLDPRHQFDVMKSLRASADKGVLVIVVTHDLGLAARFADTVLVFSDG
                     RLVSQGAPAEALSETTMGEVFRISAYRAEYQCEAVIVPWAEI"
     gene            2257..3003
                     /locus_tag="VN968_04870"
     CDS             2257..3003
                     /locus_tag="VN968_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL28606.1"
                     /translation="MNVCTDQLLIPLADPEQILGLSRYSRDRFESWAADDAQRYRILS
                     GGAEDILVLRPDVVVASLFDKRSTRELLKQKGLRLAEFAVPRNLEEAKAQIRQMGEIA
                     GHPDRAVAEIARLDAAITRARGAVVEKHYSVLPLSRRGWVAGSDSLVSSLLAETGLTN
                     AAGDLGVGAGGFASLEAVVNARPDFLLVPDSGDRAEDDGHAFLLHPALERFYPPQKRI
                     VIPDRLTVCGGVMLAQALDVLVAELKRVER"
     gene            complement(3118..4158)
                     /locus_tag="VN968_04875"
     CDS             complement(3118..4158)
                     /locus_tag="VN968_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL28607.1"
                     /translation="MIFRQLFDSVSGTYSYLLASRAGGEALILDPVLEKVDRYCQLLR
                     ELDLRLVKAVDTHLHADHVTGLGELRDRTHCVTIMGEQSKADVVAMRVGDGDRVMIEG
                     LGLDVMYTPGHTDDSYSYLMGDRVFTGDTLLIRGTGRTDFQNGSARAQYDSIFNRLLK
                     LPDETMVFPAHDYKGDTVSTIGEEKRFNPRLQVRSVDDYIELMGNLKLPNPKMMDVAV
                     PANMHVGLHQEDLARQGLSLTAREAIEGLGRPDILLVDLRENSERAKHGMLPGALHAP
                     YPAIGESLRPGGMLREVAAATGRRIVFFCAFGERSAMAVAAAKDAGLANTAHIAGGID
                     AWKKAGGPVQTG"
     gene            4316..5002
                     /locus_tag="VN968_04880"
     CDS             4316..5002
                     /locus_tag="VN968_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HXL28608.1"
                     /translation="MAEYVVEFGKDGRPIEADGRLDAAAFHRNHRPIRAVLERFLAGK
                     SGDVVEAGSGTGQHAVDFARHLPEITWWPSDLNEQHLKSILAWRAHAGLANIRSPLRI
                     DLSNPQWCAEMHDAGGPANLLAVFCANVIHIAPWRVAEGLFAGAGRYLRTEGLLFLYG
                     PFKRSGKHTAPSNAAFDNSLRDQNGEWGVRDIDDLEKLAASAGLRLVEIVEMPANNLI
                     LVFERSKRPS"
     gene            5059..5421
                     /locus_tag="VN968_04885"
     CDS             5059..5421
                     /locus_tag="VN968_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXL28609.1"
                     /translation="MPSRERLNEFIAVVESGDHAGAIERYYTEDSSMQENAAAPRVGR
                     DLLVAHERGVLARMSHVYSKAVSSVVEGDNVAIHWIFELTDKSGKVHRTDEVSLQEWR
                     GDKIFRERFFYDPSRPKT"
     gene            complement(5602..6099)
                     /locus_tag="VN968_04890"
     CDS             complement(5602..6099)
                     /locus_tag="VN968_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL28610.1"
                     /translation="MPETSTPIIAGTRIGHVHLKVADLDRALGFYCGVLGFELMQRLP
                     SGAAFVSAGGYHHHIGLNTWESKGGHPPPPGTTGLFHTAILYPTRPALADALYRVMQA
                     GIHLDGASDHGVSEALYLRDPDENGVELYCDRPKEQWPRKPDGSLAMFTRRLDLDELL
                     RQREA"
     gene            complement(6302..>6833)
                     /locus_tag="VN968_04895"
     CDS             complement(6302..>6833)
                     /locus_tag="VN968_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXL28611.1"
                     /translation="LSPGEMNEDQKKTYDESIASKRGSPPPPMMAWLNSPEMARHATR
                     LGAFLRFDTIFPAKLSEIAILVTARHWTSHYEWYAHKRLALKGGMDPQIIEDIRNRRM
                     PRFEDPKAQTIYDVAKSLHEGHGLSRALYDEAVKMLSERGLVEIIGLCGYYTMVSMTL
                     NTFEFDLPEGQVSELA"
BASE COUNT         1111 a         2126 c         2245 g         1351 t
ORIGIN      
        1 tagaagtttg tcgagatcgg agtcaatccc gtcattggct gcccgtaatc ctgactggac
       61 gagtgcgcct tttttgccgc atgacctctt gcctgaccgg aatcggcacc agggctgcct
      121 ttttgaggac gattaatgag cgttcagaac gtcatggtga cggctgacat cgcggcgcgc
      181 cgccgctccg gcgtgctggc ggggctcctt atcctcgtcg tgctgctggc gctgatctcg
      241 ctcgggatcg gaccggtgcg gctgtcgcct gttgccgtga tcgatgcctt gttcggcggc
      301 ggcagcgatg tgcagcaggt gatcgtgcgc gagattcgcc tgccgcgcac gatcctggga
      361 tttgcgatcg gcgcgatact cgggctgtcc ggcgcggcat tgcagggcct gctgcgcaat
      421 ccgctcgcct cgccctcgct gttcggcgcg ccgcaatcgg cggccttcgg cgcggtactg
      481 gtgatcgcgc ttggtcttgc cgatgtgcga tcctacgcgc ttccggtcgc cgcgatcgcc
      541 atggcgttcg cctcggtatt cgtgctgctc acgatcgcag gtcgcaacgc tggcttgctg
      601 attctcattc tggcgggcct tgcgatctcg agcctggcgg gtgccgcgac cgcgctggtc
      661 atgaacctgt cctccaatcc gttcgtggtg ctcgagattg cgttctggct gctgggctcg
      721 ctggaagatc gcagctttcg ccacgtcgtg ctggcgctgc ctttcatcgt gacgggcgcg
      781 atcgtccttt tgagccagcg caacgccttt cgcgcgctca gccttggcga ggagaccgcg
      841 caaagtctgg gcgtcgatgt cggccggcta agactgctgg tgattacggg cgtcgcgctc
      901 ggcgtcggcg gcgcggtcgc cgtcaccggc accattggct tcatcggcct ggtcgcgccg
      961 catttgatgc ggcccctgat cggccacgat ccaacgcggc tgttggtgcc gagcgcgttc
     1021 acgggcgctg cgctgctgct atcagccgat atcgccgtgc gcatcatccc ctcgaccacc
     1081 gatatcaagg tcggcgtgct gacctcgatc atcggcgtgc cgtttttcct ctatctgatc
     1141 atgcgcgaac gccgcgcgct cggcggaggc gtcgcatgac cgcatcagcc tttctcaacg
     1201 cgtggggtgt gaacgtgaca ctggccggcc gcctcgtgct caacgatgtc tcgcttgcgc
     1261 tgtcatcagg tcatctggtc gcgctggtcg gacccaacgg cgccggcaag accacgctgt
     1321 tgcgcgccct cgcgggactg gtgccctccg aaggcgtcat cgaagtcggc ggcgacgcgc
     1381 tgtcgtcgct cgccttgcgc gaacgcgccc ggcggttcgc ctatctgcca caaggccacg
     1441 tcgtgcactg gccgctggcg gcccgcgaca tcgtggcgct cggccgttat ccgcatggcg
     1501 cgaccgaccc ggcacgactg acaccgaaag atgcagaggc ggtgttgcgg gcgatgcagg
     1561 cgaccgatgt ggtggaattc agcgagcgcc gcgtcaccga actgtccggc ggcgagcgca
     1621 gccgcgtcgc gttggcgcgg gtgcttgcgg tggaagcgcc ggtgatcctg gcggacgagc
     1681 cgaccgcatc gctcgatccg cgtcaccagt tcgatgtgat gaaatccttg cgcgcgagcg
     1741 ccgataaagg cgtgctggtg atcgtggtca cccacgatct cgggcttgcc gcgcggtttg
     1801 ccgacaccgt gctggtgttc tcggacggcc ggctggtgtc gcaaggcgct cccgccgagg
     1861 cgctctccga aacgaccatg ggcgaggtat tccggatcag cgcctaccgc gcggaatatc
     1921 agtgcgaggc cgtgatcgtg ccctgggcgg aaatttagag cgggatggct tgaattgaat
     1981 cggcatgcct atctcagatc cgtcatcctg aggcgcgagc cttgcggtgc acttgcaccg
     2041 ctgggcgagc ctcgaagggt gagcggcccg ggccgtcgtc cttcgagacg gccgcgctgc
     2101 ggcctcctgc gggtgaacgc aattgcgttc atcccgggga tgacggggaa gcacctccaa
     2161 tttccattcc gatcgatcgc gctcgcagct cttgtggcgc tcggcggcgg cctggttgcg
     2221 cctgcgcgcg cggccgcttt gccgcgcatc gtctcgatga acgtttgcac cgatcaattg
     2281 ctgataccgc tggccgatcc ggagcagatc ctcggtctca gccgttattc gcgcgacagg
     2341 tttgaatcct gggcggccga cgacgcgcag cggtaccgga tcctgtcggg aggcgccgaa
     2401 gacatattgg tgctgaggcc cgatgtcgtt gtggcgagcc tgtttgacaa gcgatcgacg
     2461 cgcgaactcc tgaagcagaa aggccttcgt cttgccgagt tcgcagtccc ccgcaatctc
     2521 gaggaagcga aggcccagat tcgccagatg ggcgaaatcg ccggacatcc cgatcgtgcg
     2581 gtcgccgaga tcgcccggct cgacgcggcg atcacgcgcg cccgtggcgc cgtggtggaa
     2641 aagcactatt cggtgctgcc gctgtcgcgg cgcggctggg tcgcgggcag cgacagcctt
     2701 gtcagctcgc tgctggcaga gaccgggcta acgaatgcgg ccggcgatct cggcgtcggc
     2761 gcgggcggat tcgcctcgct tgaagcggtc gtcaatgcaa gaccggattt tcttctggtg
     2821 ccggactcag gcgaccgcgc cgaggatgac ggccacgcct ttttgttgca ccccgcgctc
     2881 gagcgcttct atccgccgca aaagcgcatc gtgattccgg accggctgac cgtgtgcggc
     2941 ggcgtcatgc tcgcgcaagc gctggatgtg ctggttgcgg agttgaagcg ggtggagcgg
     3001 tagaaggcct gtcgtcattg cgagcgaagc gaagcaatcc acaatcttga cccggagaaa
     3061 aactggattg cttcgtcgct ttgctcctcg caatgacggc aatcgagtag ggtttcctca
     3121 ccccgtctgc accggtccgc cggctttctt ccaggcgtcg atgccgccgg cgatgtgggc
     3181 ggtattggcg agtcccgcgt ccttggcggc ggcgaccgcc atcgccgagc gctcgccgaa
     3241 cgcgcagaag aaaacgatgc gccgaccggt ggctgccgcg acctcgcgca acatgccgcc
     3301 gggccgcagg ctttcgccga tcgcggggta gggcgcatgc agcgcgcccg gcagcatgcc
     3361 gtgcttggcg cgttcgctgt tttccctgag gtcaaccaga agaatatcgg gccggccgag
     3421 gccttcgatc gcctcgcgcg cggtgagcga caggccctgc cgggcaagat cctcctgatg
     3481 caggccgaca tgcatgttgg ccggcaccgc cacgtccatc atcttcggat ttggcaattt
     3541 cagattgccc atcagctcga tatagtcgtc gaccgagcgc acctgcagcc gcggattgaa
     3601 gcgcttttcc tcgccgatgg tggagacggt atcgcccttg tagtcgtggg cgggaaaaac
     3661 catggtttcg tcgggcagtt tcagcagccg gttgaagatg gaatcgtatt gcgcccgggc
     3721 gctgccgttc tggaaatcgg tgcggccggt gccgcggatc agaagcgtat cgcctgtaaa
     3781 tacccgatcg cccatcaaat agctgtagga atcatcggta tggccgggcg tgtacatgac
     3841 gtccaggccg aggccctcga tcatcacccg gtcgccgtcg ccaacccgca tcgccaccac
     3901 gtcggccttg ctctgttcgc ccatgatggt gacgcagtgg gtgcggtcgc gcagttcgcc
     3961 gagcccggtg acatggtcgg cgtgcagatg ggtatcgacg gccttgacca ggcggagatc
     4021 cagttcccgc agcagctggc agtagcgatc gaccttctcc agcaccggat cgaggatcag
     4081 cgcttcgccg ccggcgcggc tggccagcag atagctgtag gttcccgaga cgctgtcgaa
     4141 tagctggcga aagatcatgg ctggtgctgc cgccttgtcg cagaacaata gcgcaatttt
     4201 ttggtgcgca ccgcatcttt taggagatta tagtcgtcgc cgacaagcgc gcgttgcaca
     4261 tgttgttcag gtgctcatgg aaatctgtca cggcgatctg aaatgagagg acatcatggc
     4321 tgaatatgtg gtggaattcg gcaaggacgg ccggccgatc gaagccgacg gccgtctcga
     4381 cgcggcggcg tttcaccgca accatcgccc gatccgggcg gtgttggaaa gattccttgc
     4441 cgggaaatcc ggtgacgtgg tggaggcggg cagcggcacc ggccagcatg cggtcgattt
     4501 cgcccgccat cttcctgaaa ttacctggtg gccgagcgat ctcaacgagc agcatctcaa
     4561 gagcattttg gcgtggcggg cacatgccgg gcttgccaat atccgctcgc cgttgcggat
     4621 cgatctttcc aatccgcaat ggtgcgccga gatgcacgat gccggcggcc ccgcaaattt
     4681 gctggcggtg ttctgcgcca atgtgattca catcgccccg tggcgcgtcg cggaagggtt
     4741 atttgccggc gccgggcgtt acctgcgaac cgagggactg ctgttcctct atgggccgtt
     4801 caagcgcagc ggcaagcata ccgcgcccag caatgccgcg ttcgacaata gtcttcgcga
     4861 ccagaacggc gaatggggcg tgcgcgacat cgacgacctg gaaaagctcg cggcaagtgc
     4921 cggcctcagg cttgtcgaga tcgtcgagat gcccgccaat aatctgatcc tggtgttcga
     4981 gcgatcaaag cggccttcct gacgtgagat tgcgccaata gattgagtct cgtcatcgcg
     5041 agcagcgctg gaggttcgat gccatcgaga gaacgcctga atgagtttat cgccgttgtc
     5101 gagtccggcg atcacgccgg cgccatcgag cgctattaca ccgaggattc cagcatgcag
     5161 gaaaatgcgg cagccccgcg ggtcgggcgc gacctgctgg tcgcccacga acgcggcgtt
     5221 ctggcgcgca tgagccatgt ctattccaag gcggtctcgt ccgtggttga aggcgacaac
     5281 gtcgccatcc actggatctt cgagctgacc gataaatccg gcaaggtgca ccggaccgac
     5341 gaagtcagct tgcaggaatg gcgcggcgac aagatttttc gcgagcgctt tttctacgat
     5401 ccgtccaggc cgaagacgtg atggcgaggg cgtgacgctt cggcgacggt cgaagcatcg
     5461 cgcttgctga tttggtattt gttggtgaga ggacgaggcg ggcacccaaa acgacgacct
     5521 catcctgaag gagcggccgc cgcgtctcga aggatggcct catggttcga gacggcgcct
     5581 gcgcgcctct cagcatgagg gtcaggcctc ccgttgccgc aagagctcgt cgagatcgag
     5641 ccggcgcgtg aacatcgcga gcgagccgtc cggcttgcgc ggccattgct ccttgggccg
     5701 gtcgcaatac aactcgacgc cgttctcgtc ggggtcgcgc aggtaaagcg cctcgctgac
     5761 gccgtggtcg ctggcgccgt cgagatgaat tcccgcctgc atcacccgat acaatgcatc
     5821 ggccagcgca ggccgcgtcg gatacaggat cgcggtgtgg aacaatcccg tcgttcccgg
     5881 cggcggggga tgaccgcctt tgctttccca ggtgttcagc ccgatgtggt gatgatagcc
     5941 gccggccgaa acgaacgccg cgcccgaggg caggcgttgc atcagctcga accccagcac
     6001 gccgcaataa aatcccagcg cgcgatcgag atcggccacc ttgagatgga catgcccgat
     6061 gcgggtgccc gcgatgatcg gtgtggaggt ttcgggcatg gcgtgtttcc ttcttggggt
     6121 cgtacagcct cggaattatc gtgccccgga cgctgcgcag cgcgcaagcg gtgcgccgct
     6181 gatccggggt catcttgttc aacttcgagt gggtcccggc tctgcggagc agcgtgaccg
     6241 gacgatgctg cgcatcgccg ggagaacgct gcaccgcgtc cggggcacga cggtactcgc
     6301 ctcacgccag ctcggacacc tgtccctccg ggagatcgaa ctcgaacgtg ttcagcgtca
     6361 tcgacaccat ggtgtaatag ccgcacaacc cgatgatctc gaccagcccg cgctcactga
     6421 gcattttcac ggcttcgtcg taaagcgccc tggaaagtcc atggccttcg tgcagcgatt
     6481 tcgcgacgtc gtagatcgtc tgcgccttgg ggtcctcgaa acgcggcatg cggcgattgc
     6541 ggatatcctc gatgatctgg ggatccatgc cgcccttcag cgcgagccgc ttgtgggcgt
     6601 accattcgta atgcgatgtc cagtgccgcg cggtcaccag gatggcaatc tccgacagtt
     6661 ttgccggaaa tatcgtatcg aagcgcagga aagcgcccaa acgggtggcg tgccgcgcca
     6721 tctccgggct gttgagccag gccatcatcg gcggcggcgg gctgccgcgc ttgctggcga
     6781 tcgactcgtc gtaggttttt ttctggtcct cgttcatttc gccaggcgaa agc
//
LOCUS       DATNOI010000133        29799 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_122419,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000133 DATNOI010000000
VERSION     DATNOI010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 29799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29799
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122419"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VN968_04900"
     CDS             complement(<1..563)
                     /locus_tag="VN968_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018245289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HXL28612.1"
                     /translation="MTIKVLKPGLATTVQDLGRPGYYHIGIPLSGGMDRHALAAANLL
                     VGNAEGAAVLEAVFMGPELEFTDDAMVAITGAELPPKLDGVSRETWASFKVKRGQKLS
                     FDFLKKGARAYIAVAGGIDVPVVLGSRSTYALGALGGFKGRKLEAGDELPVGAATTAV
                     EEGRTVGEQLRGLPIAMPTELRVMPGL"
     gene            complement(553..1428)
                     /locus_tag="VN968_04905"
     CDS             complement(553..1428)
                     /locus_tag="VN968_04905"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HXL28613.1"
                     /translation="MQTRFSFGGDEHIFAEVDDSMSLEAFFKSLFITNAVRDARIKGV
                     IEICPANASYQVKFDPDLIKPDDMLAELKRMDSAAEKSEPVIATRIIEIPVLYNDPWT
                     HETLMRFRERHQDPNGTDLEYAARINNLDGIDAFVEAHSSAPWFVSMVGFVAGLPFLY
                     QMVERQRQIQAPKYLRPRTDTPKLTVGHGGCFSCIYSVRGAGGYQMFGITPMPIYDPN
                     QKISYLRDFMCLFKPGDIVKWKPIDRPAYDAAVADVEAGRFSPIIRDVSFSLTEFNRD
                     IDGYNHKLEGVLHDH"
     gene            complement(1513..2874)
                     /locus_tag="VN968_04910"
                     /pseudo
     CDS             complement(1513..2874)
                     /locus_tag="VN968_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
     gene            complement(2891..3133)
                     /locus_tag="VN968_04915"
     CDS             complement(2891..3133)
                     /locus_tag="VN968_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HXL28614.1"
                     /translation="MASQQIFSPLPGIFYRKPAPDQPDYKSDGDTITEDDTIGLIEVM
                     KSFNEVKAGAAGKIVRFLAENEEAVMAGQPIAEIDV"
     gene            complement(3179..3907)
                     /gene="pxpA"
                     /locus_tag="VN968_04920"
     CDS             complement(3179..3907)
                     /gene="pxpA"
                     /locus_tag="VN968_04920"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HXL28615.1"
                     /translation="MVIINCDMGEAYGLYRMGDDEGLMPQIDVANVACGFHASDFNHM
                     RKTVRLAKQHGVKVGAHPSLPDLQGFGRREMKIGREELTNCLLYQIGALKAFLDAEGV
                     VLNHIKPHGALYGMAARMEDIAEAVADAADVYKVPLFGMKGTLHEKVYRRRGHAFAAE
                     YYADLDYNAEGGLIITREHDAKDPADAAARCLRALIEGKTRTVAGNDVAVGADSICVH
                     SDTPNAVAIAEAVREAVRPYLAAR"
     gene            complement(4080..5267)
                     /locus_tag="VN968_04925"
     CDS             complement(4080..5267)
                     /locus_tag="VN968_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL28616.1"
                     /translation="MRFTRVLGVLAAASALWCTPTRAADEIVVGFATAASGFMQAYDK
                     PAQDAALIRIEEINKAGGLLGKKIKPVFADTKTDQAEGAKAGLAVLDQGADLVIVSCD
                     YDFGAPAALQAQSAGKVSFFLCAESIKAGIPGVGPYSFSGSVLAAVQGATMAEWAYKK
                     KNARSFYRLLDSWTVYNKGICDGFDWMMPHLKDSALAGSDTFKNDDASIASQITRIKS
                     LPKEPDAIMLCTMMPGAVSAIKQIRAAGIKSMILNGSGVDGSYWLNAVPDLSNFYVPV
                     QASIYGDDPNPKVTEFNKKYKDITGGDPSSQYVYPGYVLIDVWAKAVERAKTTDAEAV
                     VAELEKMNNEATLFGPRTFSKDLHHQNQGRYLIVDTEEGKPKVVDQWTISEKIPVDYL
                     VSK"
     gene            complement(5298..6371)
                     /locus_tag="VN968_04930"
     CDS             complement(5298..6371)
                     /locus_tag="VN968_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL28617.1"
                     /translation="MLQRPSVIWQTIATPVILIVVLLAIAALSYQFGSRAFNRTVVEM
                     FINIMVVAGLYVFVGNSGLLSFGHISFMCLGAYMTAWLTIPPVMKSITLKGLPTWLLH
                     TKLSMWVATPISGVFAALFALIVGRVIMRLSGIAASIATFGLLGVVNNVYSNWDSVTG
                     GQGSIVGTPATMNVWNGWLGAAVAIAIAYLYSISRSGLALRAARDEAIAASASGIDIV
                     RERLVAFVVSAFIIGLAGALYAHFLSIVNPGSFYLRTTFITLSMLVVGGMYSLSGAVA
                     GVVVISALIEMFRDLEKGVSLGGLTIALPNGVQEIAIGIITIVILMYLPTGLTRNREF
                     SWRGWPLQRHLTQPVQTALKELN"
     gene            complement(6374..7288)
                     /locus_tag="VN968_04935"
     CDS             complement(6374..7288)
                     /locus_tag="VN968_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL28618.1"
                     /translation="MTELLQIVFDALSLGSLYALGALGIALIFGVMRLVNFAHGDFIA
                     FCVFAMLWPSINAAAIVFAGNLPFYLLIPLLLLVGGALSVLSEVIVFRRFRNANPATM
                     MIASFALGFAIRNFLLMMYSSRPKSITLWPRLSLPVEVLGARIPMLQVVTIIITLVVL
                     VALTVFLKRTRYGLEMRAAAENFTMARMLGVRANRVILLAFAISGMLAAAIGLVLATN
                     SGTADIGMGANLMLIAFIATVIGGLGSVPGAVAAGFLIGGASVVFQATLPHDARVFRD
                     AFVYGAVIVVLLVRPQGLFAPKSAKQRV"
     gene            complement(7291..8013)
                     /locus_tag="VN968_04940"
     CDS             complement(7291..8013)
                     /locus_tag="VN968_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28619.1"
                     /translation="MTLLAVNDVTVHYGRLTALRGITLAIAEGEVLFVTGPNGAGKST
                     LLNAIAGVVPPVSGSITMDGAEVTGAPPEQIARRGFSLVPEGRNVFGALTIEENLKVG
                     AGMRADKQEVARDLESVYHEFPMLAERRHAAAGMLSGGQQQMLVIGRALMAAPRLMAI
                     DEPSLGLAPKIIDQVYEILVRLRAQRKLTLLIVEQSSTRAMMTGGRMILMRGGCIVLE
                     GEAGDMVKDERLKQAYFGFGDH"
     gene            complement(8010..8762)
                     /locus_tag="VN968_04945"
     CDS             complement(8010..8762)
                     /locus_tag="VN968_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28620.1"
                     /translation="MVARDVSVQFEGLKALSDVTLAVPRGRITGLIGPNGAGKTTLIN
                     VLTGFQRVGAGTVELEGETVNGVAAHKLRRKGVARTFQSGRLFRDLPVVDNLEVTGVG
                     LGQTRRAAIAEAERMMDWLGISHLAGAIAGALPYTDERRVAIGRAIMCDPRYLLLDEP
                     AAGMSEHESHDLAAIIKRIAGELGVGVLLIEHNIGLVLELCERIFVLDSGEIIEVGEP
                     DKIRNSDAVRHAYMGTQRNEVIPVLANGEAVA"
     gene            8889..9809
                     /locus_tag="VN968_04950"
     CDS             8889..9809
                     /locus_tag="VN968_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXL28621.1"
                     /translation="MALTFRQVHHFVATAETGKVSAAAAGLNVTQSAVTASIKALEAE
                     LGQKLFDRHSNGVTLTFDGQQFLQRARTIEASVSDAMRGPHRWGTQVDGTVDVAVSYT
                     VAGYFLPPLLSRFWRAFPGITVRLHEFERDAIEHNLMSGSVDAAVMLVSNLHDRRSIC
                     SRLLLRSPRRLWTCANHPLLHKGGVRLADLAGEPFLMLTVDEAERSAMRYWQRTPHVP
                     NVIFRTLSVEAVRSMVATGMGITILSDMVYRPWSLEGHRIDLKTLDDEVDTMDVGLAW
                     KSKARLSLAARAFCEFVALAYPGPDPVHFD"
     gene            9828..10256
                     /locus_tag="VN968_04955"
     CDS             9828..10256
                     /locus_tag="VN968_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28622.1"
                     /translation="MDGLIFTMESIESYGPDKLVKHSTDSVTFGSFVIAYSMNRTVWN
                     RLPDDVKKAIDEASEATVAKACADIEAEQPESHKSLERAGLSFDPISEDAARKIKARL
                     AEVAKEWAAALDSRGKPASAVLKEYESLLAASPPNYPYVA"
     gene            10373..10510
                     /locus_tag="VN968_04960"
     CDS             10373..10510
                     /locus_tag="VN968_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28623.1"
                     /translation="MPFGCSVLVLRLALQLVGNVVSLASNRDLYPLPPITGVGEAKTF
                     E"
     gene            10516..11277
                     /locus_tag="VN968_04965"
                     /pseudo
     CDS             10516..11277
                     /locus_tag="VN968_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            11613..14825
                     /locus_tag="VN968_04970"
     CDS             11613..14825
                     /locus_tag="VN968_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL28624.1"
                     /translation="MRCAKCGATSREGKRFCADCGAALVAVVRSEPASRSVAGAVAPT
                     ERIQSERRHLTVLFGDLVNSTRLAAEYDPEEWRDIVTECLEAAGDAIKDLGGYVARYM
                     GDGVLAYFGWPTASEDDAERAVRAGLTIIKAVAPLNRRLTGKAGSELAIRVGIHSGWV
                     VIDELGTNKVEVFGDTPNIASRVQARCAPNSVLMTGAVHDLVAGQFIVEDCGAHPLAG
                     IARAVQLYRAIAPSGIRRSWRRVGVRGPTPFVNRQRMFDVLWSSWLGARNGAGRYVFV
                     TGEPGIGKSRLVEEFRSRIKDAHIWMECAGERFSESTPFHAVIKLLEQALGGEKEESA
                     EGRIRQLNRALRLSGLDSSEMVPLIAEMLKLPPSREYPLPQFAPEEARGRLLAGLAGW
                     VLKLAKLQPLILAIEDLHWVDPSSIELIGMLIEQSADVPLMLMATARPEYQPPWESRA
                     TDKSIVLGRLSNDEIAEMIAGSSDVEGLTEEVVAGVVKRSDGVPIFAEELLSFVLEGE
                     GDPTANNIPTTLLDSLTARLDRLGPARRVAQVAAVLGREFDYPLLRAVVPGSDQDVQS
                     ALDALIRADLIYTQGRPPRATYQFKHALIRDAAYEGLLKTERRELHARVARTIGEQFP
                     ASAAAQPVLLARHWTEAAEAEPAIAAWKNAGQAALARCAFKEAEEAYRQASALLNRLP
                     PSETRDRDELDLCSALVRVLQVTKGYSAPETMQLGARARVLAERLGDLSQLFRLRART
                     WASIFFTGDYAAAASLAEQILEIALTESSNSSHLFFAHYAQVQARFYAGDLAGVEDHF
                     AKLSPLLDTRSIAAAADLILPIGVASRAAWQQGRVDVARERMSRAMNLARKSQDPYAI
                     AMALHFEGNLHWCLKAPRRVEVIGNRLLSLSEEHGLSYASDLARVLLGWAKSELGHAA
                     EGVELINQALAGFAAAGAKVAITYFLTLLAQAQACAGDAESGLRTIEEALAANPQELI
                     WRAYTLTCRGELRFKLGQPAMAEADFRDAIEMARSLEHKAWQLRAATSLARLLMRRGD
                     PLAARGSLVPVYSSFGEGFETPDLREARLLLEEMAHLPAT"
     gene            complement(14980..15912)
                     /locus_tag="VN968_04975"
     CDS             complement(14980..15912)
                     /locus_tag="VN968_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXL28625.1"
                     /translation="MSKATTESIIAQLGFTEEMQQLREILDSWIRGANEQIKPLLEWQ
                     FITSSKYFRPLTVFSVYRATTDEPVPNRLIYSAAVIEMMHNMTLIIDDIVDGSETRRG
                     RPTLHCQFGLLQALMASGYIVADGFRMTAGDRIDIRLMSDLIKRLGAAECLQWNVRRQ
                     LLSVEDWLNIAGEDTGSMFETCACVATRDDLLRKFGGLLGVLYHGCDDVGDVKGLESL
                     GGGGEEDLRDGILTLPASIAIKDPVIAELFCKPVRSDDELAQISRAVKAAVPAAEDRL
                     DQLAAEAKWEAHLHAANPAPLYALVDHTRELSRR"
     gene            complement(15979..18039)
                     /locus_tag="VN968_04980"
     CDS             complement(15979..18039)
                     /locus_tag="VN968_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL28626.1"
                     /translation="MTSTASQSKTSKPPMPAEKEVAIIGAGIAGLTCALRLSKRGYKV
                     TLYEKDGMLGGDLSSKLSNGMYHDVYTHLFCDWYVNFWRIVEDDLKIKRHDAFEPRMS
                     VKLLDPPSSPQHSADNTPRYTELKNPNTLKNALDNLRSGALPPPDMFLVGFTLLDLAS
                     QPFHRSNLLEQQTVNGFLYSRPYATLDCADLHNTILNEVWCISSSDTSAAAYKDFVKH
                     VLGSSGLPFAWLLRGSLEEMLIKPWQESLKNDCNIRASVKVTAVEVGDDDKLQLTLSD
                     KTKATHANVVFAVPAPELAKLVMSGRPGRCVVERLPALSELQRLCTASILVVTVFFKE
                     KLPNVPREHVGLARSRGYLTFIDISQLWTSLSNIKEQHSVLILAASDAYAYPSANEEE
                     WAHLMIQELAGYLPAVKPGRRWGDADSNIDYSKSFPQNNRAHTLFLNDMNSEQVLLEP
                     SYPDLRNVFFAGDFCRNEVKMATVESAVLSGLHAARAVQMKAEGKSDITVEPGVIPSS
                     AEFSVAKLALLPLAYGATAWAAVNVALRDLANGQAETPEGALAPVTTLALIPLRYVAD
                     WVASLETLGVALLSPAGEPDHSSSTVQQGLRATARGMLAASDHLCNLASKRSADKPRG
                     LASLLTDLLKVVSTAASEPPAARPGGAGPTLRSALAEGSGLLRGSGRHRPRLEYVRRH
                     RAKL"
     gene            complement(18067..19548)
                     /locus_tag="VN968_04985"
     CDS             complement(18067..19548)
                     /locus_tag="VN968_04985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL28627.1"
                     /translation="MSVSDLYDVIVVGGGPAGSTMAWSLARRGVRVAVIERAIFPREK
                     VCGDFVEPAGLRILEAMGVREALQASSPLPITSTRIYFGPRLGFRGVIPYYQAEHGLP
                     PHGFVVPRHILDHHLLERARAVSAKVYDGCAARGIRREDGWIHVDVRGRESDFSLRSR
                     LIVGADGAESLVARSFNLEMTDRAHISIAQRAYVEGVSVQDGEATVWFDEDVVPGYGW
                     MFPMSGGRANVGIGILSETSHRHGHSVPKDFVATIERLKIRHPGCARIKVVSKAIGGV
                     VKMYGGVGPNYFDGGVLIGDAGSFADPMTGEGITQGMESALIASSTLLKALELGRFDA
                     AFLANFERDFRSYFDPGMLFLGFCAAAMRNWHFREFWMRSALRGCERAMADGDFARIA
                     GAGFGGLNVHPLGILRQIGASILRYLGEGTMNTLRSFVIGTPIGGDAMLGDLVAWQSA
                     WKRSVSADPDWHAAWLADVVKKAVQLGPSLLTQENPRVRGLLI"
     gene            complement(19999..22470)
                     /locus_tag="VN968_04990"
     CDS             complement(19999..22470)
                     /locus_tag="VN968_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL28628.1"
                     /translation="MIGVNDKIWFLREGLFAKYVMSLVGLVVFVLAVNGATETWISYR
                     ATKTTLIDAMSEKAEATARRIEQSISELERQISWVTRSSAKTYSGVVTLEDHHADYVQ
                     LLNQVPAVSQLTLLDQNGREQLRLSRTAIAVGSGTDLSRDVQMAETVARGVSDAPAFF
                     RGSRPLMSIAVSHSGSDPAAAFTVAEIDLGLLSDFFGDAQVGKTAFAYVIDPKGQVLA
                     SSARGPDVAKDLSKLPQVAALIRPNGQALASGTDVDRHAVLTASNAIPKLGWFVCFEQ
                     PTAQALAPIRDQLVRIALLISLGLGVAILAGTVLARRMLVPITALRTGARRLGAGDFS
                     HRIDVHTKDELEELADQFNSMAGKLHETYSGLESKVEERTRDLAQSVNELKVLEEVGR
                     AVASSLDLNAVLPTVAARALEITHADAVLIFGYDAARHQFNLTEAIGIDKSTEQARLA
                     IDADGSVLGDAAATGEPVAIPNLADAADHPLRDAAIAAGFHSVLVVPLVDQKGILGSL
                     VVLRNSAGEFSENLIGLMRTFAHQSVLAMRNARLFTEVDQKGRELATAHSTVQQQADQ
                     LLNWNKSLEERVEKQLGEIERIRKLERFLAPQVAQLIASSDGHDSLLDSHRREVTVVF
                     CDLRGFTAFTESTEPEEAMAVLREYHAALGELIFRYEGTLDRYAGDGVMILFNAPIEF
                     ADHTRRAVKMAVEMRDTIGLLTQKWRNRGHSLGFGVGIALGYATLGQIGFEQRLEYAA
                     IGSVTNLASRLCDEAQANQIVVSRRVFGMVEPWVEGKPLDDLNLKGFNHPVLAVEILS
                     WRHEAEAGDVKVASAEAPRRRQQPL"
     gene            complement(22720..24726)
                     /locus_tag="VN968_04995"
     CDS             complement(22720..24726)
                     /locus_tag="VN968_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXL28629.1"
                     /translation="MKALKIAGAAVAAVIVIVAVLLIVGIPSGFLTSAIQDRVERETG
                     YRLTIAGSTKIGLWPSLNVTVNDVTLEAPKDRDASNRVTVGSVQADMTLKSVWSGHPQ
                     ITELVITRPVLHHPLLRERERLPAPAAGPAASSDGKDANALAIDRVTIVDGTMVMFNL
                     RDRVENRIEGINAEALIGDDRKIKVTGRARASEHPLKFAITATVPAPPLQRQNIPVEL
                     TLDASGLLQAPLAAKAEVRLNGSIVMINGVTGTLGDGAFNGWASVDLSSKPLVKLDLD
                     FQRLEVATSKERATSASLGSQPWSNASIDLTGLNYVDAQLRVSAAELNIGEARFAPVA
                     IDTAVAAGVLKTSFSNLGVYGGQASGQLIIDVTSGSPGYAFSGDLVGVRALPLLRSAA
                     DFDKLDGKMQAKMTLRSAGNSQRLVMSNLSGTVFAVFQDGAIRGINVAAMIRSLTSGT
                     LSGWQEGKEQTTDLTQLSASFRIDKGQATTADLNLVGPLVKITGAGTIDLAAKALAFR
                     VEPKLVLTTEGQGRTSDPVGLGIPVMIDGPWAEPRIYPDMAGILDNPDAAYAKLKEMG
                     KGLFGSSGGLGSVLGGLGGSGGSGSSGGSGSGSNGLADQLGSTLGNLLQQGLGQRRSQ
                     SRTLAAPESPRPDAPPAAAQIDPAPEVSQDSQPMNDVLKQLFNR"
     gene            24974..25648
                     /locus_tag="VN968_05000"
     CDS             24974..25648
                     /locus_tag="VN968_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXL28630.1"
                     /translation="MSKATEFAVILKMNPMFADLGTDELARISGLCHTQQLGLGEVLF
                     QKGDSGDALFGVRRGQIRIETGAADGSRLTLNFMGPGDLFGEVAVLDGQSRTADATAG
                     EPTELFVLRREDFLSHLEREPRVAIKIIQLLCQRIRWQSERMEESVLQPLPVRLARRL
                     CALATDYGSEVHISQEQLGVFVGAARESVNRQLQLWRKEDILDLQRGRILLLNMNRLT
                     AVARNE"
     gene            25920..28085
                     /locus_tag="VN968_05005"
     CDS             25920..28085
                     /locus_tag="VN968_05005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXL28631.1"
                     /translation="MDLAFSNMAARSKTSPARWRRPFLSWMDGIEAGWAIPLLLIGFT
                     VVWLAFLIIAYLDGDLHYDVLETWTLGRGIDWSYSKHPPLMGWVARAWTSVFPLTNWS
                     FQLLALTNAAFALWTVDLISRRFVRGDKRMIVLLLLMLLPIYQFHAQRFNANAVLLAA
                     WPLATYCFLRSFETREIRWAAAAGATAALAMLGKYYSVFLIGSFVFAAICHPQRRAYF
                     TSWAPWVSLVTLFTALLPHLNWLLVNGAPPVVYALTRHTGKDFAPALLESVVFLLGVA
                     MILAIPAATWALMAQDRIKNFSEDFWAMHSGLLLLFLVSIGTIAFPAITTVALGADMP
                     PLWALQGLFLFVILIVCGASYPIERFYSVNLTVAVIGIAVVAVVVAAPLHAFYRNGHP
                     LHEGRNFFSQAAAELTRRWHEQSDAALAAVGGDDGLALATAFYSPDHPVYQSPLVYPH
                     TETSPREASFSRGWAALCYDGDTSCIDSMERTAARAGRFVRTRFVVQSTVFGQPGASQ
                     GFAALMVPPYDEDKITPPSASSGPAIAQNGSEGASVLVRQDEPTCCTRNPRLDAAERL
                     SELSLPEISARDSDRKRARHAVAPAAAAYQANWPDRPASSATRDGFARWPIPTPPPRA
                     KSSVARSSFGARPALQACSTRGPSVSPGRERMADSTSEPLSVRFRRRFCAMAADFDER
                     MRVSQEKFDAFVGNARESADRRLQLWRRDSMLDQQRRGI"
     gene            complement(28170..28376)
                     /locus_tag="VN968_05010"
     CDS             complement(28170..28376)
                     /locus_tag="VN968_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28632.1"
                     /translation="MNQVSRTLEIDDDTGARLREMAAERGHEVAAALTEVMAMLDSVV
                     DLTRWVKSWRSAHELPRPHPRDRD"
     gene            complement(28701..>29799)
                     /locus_tag="VN968_05015"
     CDS             complement(28701..>29799)
                     /locus_tag="VN968_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXL28633.1"
                     /translation="SLDRPELRIQPRADLAARLGVSTESLSQTIRVATIGDVGPALAK
                     FDAGDRQVPIRVQLEDGARGDLQLLEQLRVPLGQHGERGGVPLSVVADIKLDQGPTSI
                     NRYDRERQATVAADLVGSSALGDATKKIYDLPVMKSLPKGVKVSPSGDAESLAELSDG
                     FATAITAGLMMVYAVLVLLFGTFLQPITILFSLPLSIGGAIVALLLTGKQLTTPVWIG
                     ILMLMGIVTKNAIMLVEFAVESIREGKRRDVAIIDAGMKRARPIVMTTIAMAAGMMPS
                     ALAFGAGGEFRSPMALAVIGGLVFSTVLSLVFVPAMFMMMDDVGALFWRFGKKLLASH
                     AESEHAADGHEPERAAEHKGPPTIVHSPAAE"
BASE COUNT         5482 a         9297 c         9298 g         5722 t
ORIGIN      
        1 taaagtccgg gcatgacgcg caactccgtc ggcatcgcaa tcggcaagcc gcgcaattgt
       61 tctccgaccg tacggccctc ctcgaccgct gtcgtagccg caccgaccgg tagttcgtcg
      121 ccggcctcga gctttcgacc cttgaagccg cccaaggcgc ccagcgcgta ggtcgagcgc
      181 gagccgagaa ccacgggcac atcgattccg cctgcgacgg caatgtaggc gcgcgcgccc
      241 tttttcagga aatcgaagga gagtttttgg ccgcgtttca ccttgaagga ggcccacgtc
      301 tcgcgcgaaa cgccatcgag cttgggtggc aactcagccc ccgtgatcgc gaccatggcg
      361 tcatcggtaa actcgagctc cggccccata aatacggcct cgagaacggc agctccctcc
      421 gcattaccca ccagcaggtt ggcggcggcg agcgcatgcc ggtccatacc gccggagagc
      481 ggaatgccga tgtgataata gccgggacgc ccgaggtcct ggacggtggt ggcaagaccc
      541 ggcttcaaaa ccttaatggt catggagaac cccctcgagc ttgtgattgt agccgtcgat
      601 gtcgcggttg aactcggtga gcgagaacga gacatcccgg atgatcggcg agaagcggcc
      661 cgcttcgaca tcggcgaccg ccgcgtcata ggccggccgg tcgatgggct tccacttcac
      721 gatgtcgcct ggcttgaaca ggcacatgaa atcacgcaga tagctgatct tctgattggg
      781 atcgtagatc ggcatcggcg tgatgccgaa catctggtag ccgccggcac cgcgcaccga
      841 gtagatacag ctgaagcagc cgccgtgacc gaccgtcagc ttgggtgtgt ccgtgcgcgg
      901 gcgtagatat ttcggcgcct gaatctgccg ctggcgctcg accatctggt agaggaaggg
      961 caggccggcg acgaagccga ccatcgagac gaaccagggc gcacttgaat gcgcctcgac
     1021 gaacgcgtcg atgccatcga gattgttgat gcgtgcggcg tattcgagat cggtgccgtt
     1081 gggatcctga tggcgctcgc ggaagcgcat cagcgtctcg tgcgtccagg gatcgttgta
     1141 aagcaccggg atctcgatga ttcgggtagc gatcaccggc tcggactttt cggccgccga
     1201 atccattcgt ttcaactcgg cgagcatgtc atcgggtttg atcagatccg gatcgaactt
     1261 cacctgatag gaggcgttag ccgggcagat ctcgatgacg cccttgatcc tggcgtcgcg
     1321 caccgcgttg gtgatgaaga ggctcttgaa gaaagcctca agtgacatgg aatcatccac
     1381 ctcggcgaag atgtgctcat cgccgccgaa agaaaatcga gtttgcatgg gggagtgacc
     1441 tcctgttgct gacggggcgg attctgttgc ggtcgttggg gtgatctgcg cggccggcac
     1501 ggcctctatt gcggcaagac ttgcagcatg tgatgccagc catggctcga gccaggccgt
     1561 atgaaagcgc ccggcctgca cgtcagcatc gcgcgcgagc gcctggtgca ggggcttggt
     1621 cgtcgccagc ccctcgacgt tcagttcacc gagcgcgcga tcgagccgtc ggatcgcgct
     1681 cgctctgtct tcatcgtgga cgatcagctt gccgagcaag ctgtcataga acggcggcac
     1741 cgtgtagccg gaatagagca tgctgtcgaa acgcaccccg ttgccgcctg gcacattgag
     1801 tgcggtgacg gtgcccgggt tcggcatgaa gccttttgcc gggttctcgg cgttgatgcg
     1861 gacctcgatc gagtggccat tgatgcgcac atgatcctgg ccgatgcgca ggcgctcgcc
     1921 gccggcgatc aggatcatct cgcgcaccag gtcgatgccg gtgatcatct tcgtcaccgg
     1981 atgctcgacc tggatgcgcg tgttcatctc caggaagtag aattcgtgac tgcggtcatc
     2041 gtacagatat tcgagcgtcc ccgcgccgcg gtagcgcacc gccttggcga gcgcgacggc
     2101 cgacgaacag agtttttcgc gaatggcagg tgtcagcgag ggcgagggcg cttcttccca
     2161 cactttctga cggcggcgct gcagcgagca ctcgcgctcg aagcagtgga tgacatcgtg
     2221 cccgtcgccc agcacctgca cctcgatgtg ccgcgcacct tcgatgagct tctcgacata
     2281 gagtccgccg tcgccgaagg ccgcgagcgc ctccgcctgc gcctgcggca tgaggtgatg
     2341 aaactcctcg gccgagcgcg cgatgcggat gccacggcca ccgccgccgg ctgccgcctt
     2401 gatcatgacc ggaaagccgg tcgtcttcac caaggcgaat gccgcctcgg gtgacccaag
     2461 gcggccgcgg ctgcccggaa ccgtgggaac gcctgcggcc gccgcggctt cccgcgcggc
     2521 gaccttgtcg cccatcaggc ggatggactg ggccgtcggc ccgacgaaga tcagcccagc
     2581 ggcctcgacc gcggccgcaa actcggcatt ttcggccaga aacccgtagc ccggatggat
     2641 ggcgtcggcg ccggtcgtcc tggctgcggc gagaatcgcg tcacggtcaa gatatgattt
     2701 tgacgcctgc ggcgggccta tttcgacgga ttcgtcagcc agccgcacag caagctgcgc
     2761 cttgtcggcc ttgctgtaga cctgcacagt cgcaaggccc aactcgcgtg ccgcacgaat
     2821 gatgcgcact gcgatctcgc cgcgattggc tatgaggagc ttatggatcg tcatggacgg
     2881 tttgaaggcc tcaaacgtcg atctcggcga ttggctgacc ggccatcacg gcctcctcat
     2941 tctcggcaag gaagcgcacg atcttgccgg ctgcgcctgc cttcacctcg ttgaacgact
     3001 tcatgacctc gatgaggccg atcgtgtcgt cttccgtgat ggtgtcgccg tcggatttgt
     3061 agtctggctg gtccggcgct ggcttgcggt agaatatgcc gggaaggggc gaaaagattt
     3121 gctgtgaagc catgagttcc tcgttctttg atcgcttcgg acttgaatcg gccgtgcatc
     3181 agcgcgcggc caaatagggg cggactgcct cgcgaacggc ttcggcgatg gcgacggcgt
     3241 tcggtgtgtc ggagtggacg catatggaat cggccccgac ggcgacgtcg tttccagcca
     3301 ccgtgcgcgt cttgccttcg atcaaggctc gcaggcaccg tgctgccgca tcggctggat
     3361 ccttggcgtc gtgttcgcgt gtgatgatga ggccaccctc ggcattgtag tcgagatcgg
     3421 cgtagtattc ggcggcgaag gcatgaccgc ggcgccgata gaccttctcg tgcagcgttc
     3481 ccttcatgcc aaacagcggc accttgtaga catcggccgc gtcggccacg gcttcggcga
     3541 tgtcctccat ccgcgcggcc attccataga gcgcgccatg cggcttgata tgattgagga
     3601 ccacgccttc agcatccagg aacgccttga gtgcaccgat ctggtagagc aggcaattcg
     3661 tcagttcttc gcggccgatc ttcatttccc tccgtccgaa gccctgcaaa tcgggcagag
     3721 agggatgggc gccgaccttg acgccatgct gcttggccaa tcgcacggtc ttccgcatgt
     3781 gattgaagtc agaggcatgg aatccgcaag cgacgttggc gacgtcaatc tggggcatca
     3841 gcccttcgtc gtcgcccatc ctgtagaggc cgtaggcttc gcccatgtcg caattgatga
     3901 tgaccatctt ccgttttcct tataccggct gccgcatcca agcgcggcat tcgcaaaccg
     3961 accctaaacc cgccgaatga gacgcctgcc gatttcaaca ggcgtcaagt tcgtggcgat
     4021 cgacccaagc agacaaaggc cgccgcgggc ccgcagcccc gcggcggcac gctgccggct
     4081 tacttggaca ccagatagtc gaccgggatc ttctccgaaa tcgtccattg atcgaccact
     4141 ttcggcttgc cttcctcggt atcgacgatc aggtaacgac cctggttctg atggtggagg
     4201 tccttgctga aggtacgtgg cccgaacagc gtggcctcgt tgttcatctt ctcgagctct
     4261 gccaccaccg cttcggcatc cgtcgtcttg gcgcgctcga cggccttcgc ccagacgtcg
     4321 atcaagacat atcccggata gacgtattgg ctggaaggat cgccaccggt gatgtccttg
     4381 tacttcttgt tgaactcggt gaccttcgga ttggggtcgt cgccatagat tgaagcctgt
     4441 accggcacgt agaagtttga caggtccgga acggcgttca gccaatagct gccatcgacg
     4501 cccgagccgt tgaggatcat cgacttgata ccggcggccc ggatctgctt gatggcggag
     4561 acggcgcccg gcatcatcgt gcaaagcatg atcgcgtccg gctcctttgg caggctcttg
     4621 atgcgcgtga tctgagacgc gatcgaagca tcgtcgttct tgaatgtgtc gctgccggcg
     4681 agcgcggaat ccttcagatg cggcatcatc cagtcgaagc catcgcagat gcccttgtta
     4741 tagacagtcc aactgtcgag cagccggtag aagctgcggg catttttctt cttgtaggcc
     4801 cattcggcca tggtcgcacc ctgaacggcc gccagcaccg acccggagaa tgagtaggga
     4861 ccgacgccgg ggataccggc cttgatcgac tctgcgcaca ggaagaagga gactttgccg
     4921 gctgactggg cctgcagagc cgccggcgcg ccgaaatcgt agtcgcaaga gacgatcacc
     4981 aggtccgcac cctgatcgag caccgcgagg cccgctttgg ccccctcggc ctgatcggtc
     5041 ttggtgtccg cgaacacggg cttgatcttc ttgccgagca gaccgccggc cttgttgatc
     5101 tcctcgatcc ggatcagcgc ggcatcctgg gccggcttgt cataggcctg catgaagccg
     5161 gatgctgccg tggcaaatcc gacaacgatc tcatcggctg cccgggtcgg tgtacaccac
     5221 agcgcggacg ccgcggccaa gacgcctaat actctcgtga aacgcattag caatccctcc
     5281 gttttctcga cttgacttca gttcaattcc ttcagagccg tctgcacagg ttgcgtcagg
     5341 tgccgctgga gcggccatcc tctccaggag aattcccggt tgcgggtgag gccagttggc
     5401 aagtacatca gaatgacgat cgtgatgatg ccgatcgcga tttcctgaac cccgttcggg
     5461 agcgcgatgg tcagaccgcc gaggctgacg cccttttcca ggtcgcgaaa catctcgatc
     5521 aacgcggaaa tcacgacgac accggcaacg gcaccgctga ggctgtacat gccgccaacc
     5581 accagcatcg acagggtgat gaatgtcgtg cgcagataga acgagcccgg attgacgata
     5641 ctcaggaaat gcgcgtagag cgcgccggcg agacctatga tgaaggcgct gacgacgaag
     5701 gcgaccagtc gctcgcggac gatatcgatt ccggaggcgc tggcagcgat tgcctcatcg
     5761 cgggcggcgc gcagcgccag gccggagcgg gaaatcgagt agagataggc gattgcgatg
     5821 gcgaccgcag caccgagcca tccgttccag acattcatgg tggcgggcgt gccgacaatc
     5881 gaaccttgtc cacccgtcac cgagtcccag ttcgaataga cgttgttgac gacgccgagc
     5941 agcccgaagg tggcgatcga ggcggcgatt cccgataacc gcatgatgac gcgcccgacg
     6001 atgagcgcaa acagggccgc gaacacgccc gagatcggcg tggcgaccca catcgacaac
     6061 ttcgtatgca agagccacgt gggcagccct ttgagcgtga tcgatttcat caccggcggg
     6121 attgtcagcc aggcggtcat gtaggcgccg aggcacatga aactgatatg gccgaacgac
     6181 aacaggccgg aatttccgac aaacacgtaa aggccggcga ccaccatgat gttgatgaac
     6241 atctcgacca cggtgcgatt gaaggccctg cttccgaact gatacgacaa ggccgcaatg
     6301 gcgaggagca ccacgatcag tatgaccggc gtcgcgatcg tttgccaaat caccgatggg
     6361 cgctgcaaca tggtcagacc ctctgcttgg cggatttggg tgcgaacagc ccttgcgggc
     6421 gcaccagcag gaccacgatc acggctccgt agacaaaggc atcgcggaag acgcgggcgt
     6481 catgcggcag cgtcgcctga aacacgacgc tggcgccgcc gatcaggaag ccggcggcaa
     6541 cggctcccgg cacgctgcca agaccaccga tcaccgtggc gatgaaggcg atcagcatga
     6601 gattggcgcc catcccgata tccgccgtgc cggagttggt cgccaggacc aaaccgatcg
     6661 ccgccgccag catgccgctg atggcaaagg ccagcagaat gaccctgttg gcgcgcaccc
     6721 cgagcatgcg ggccatggtg aagttctcgg ccgcggcacg catttccagg ccgtagcgcg
     6781 tgcgtttgag gaacaccgta agggcaacca gtacgaccag ggtgatgatg atggtgacga
     6841 cctgcagcat cgggatgcga gcccccagaa cttcgaccgg caggctgaga cgaggccata
     6901 gcgtgatcga cttcgggcgg ctcgaataca tcatcaacag aaaatttcgg atcgcaaagc
     6961 cgagggcgaa agaggcgatc atcatcgttg ccggattggc gttgcgaaag cggcggaaca
     7021 cgatcacttc cgacaagaca gacagcgcgc ccccgaccag caacagcagc ggaatgagaa
     7081 ggtagaacgg caagttgccg gcgaagacga tggcagccgc attgatcgac ggccacagca
     7141 ttgcaaacac gcaaaaggcg atgaaatcgc cgtgcgcgaa gtttaccagc cgcatcacgc
     7201 cgaagatcag cgcgatcccg agcgcgccca gggcatagag gcttccgagg ctcagcgcgt
     7261 caaagacgat ttgcagcagt tcggtcatgc tcaatggtct ccgaagccga aatacgcctg
     7321 cttcaggcgc tcgtccttga ccatgtcgcc tgcctcgccc tcgagcacga tacatccgcc
     7381 gcgcatgagg atcatccgac cgccggtcat catggcgcgc gtcgagcttt gctcgacgat
     7441 caacagggtc agcttgcgct gcgcacgcag ccgcaccagg atctcgtaca cttgatcgat
     7501 gattttcggg gcaagtccga gcgacggttc gtcgatagcc atgagccggg gcgcggccat
     7561 cagggcccgg ccgatcacga gcatctgctg ttggccgccg gagagcatgc cggccgctgc
     7621 gtggcggcgc tcggcaagca tcgggaattc atggtagacg gattcgagat cgcgagcgac
     7681 ctcctgcttg tcggcccgca tgccggcgcc gaccttcaga ttctcctcaa tggtcagcgc
     7741 cccgaagacg ttgcgccctt cgggcacgag cgaaaacccc cgccgcgcga tctgctcggg
     7801 cggggctcct gtcacctccg cgccgtccat cgtaatggat cccgaaacgg gcggcacgac
     7861 gccggcaatc gcattcagca aggtcgactt tccggcacca ttggggccgg tcacgaacag
     7921 cacctcaccc tcggcaatcg ccagcgtgat gccgcgcaag gccgtcaagc gcccatagtg
     7981 taccgtgacg tcgttgacgg ccagcaatgt catgcgaccg cctcgccatt cgctagcacc
     8041 ggaatgactt cgttccgttg cgtgcccata taggcgtgcc ggacggcatc gctgttgcgg
     8101 atcttgtccg gttcaccgac ttcgatgatc tcgcccgagt cgagcacgaa gatgcgctcg
     8161 cacagttcga gcacaagccc gatattatgc tcgatcagga gcacgccgac cccgagctcg
     8221 cccgcgatcc gtttgatgat cgccgccaga tcgtggcttt catgttcgga catgccggcc
     8281 gcgggctcgt ccagtagcag atagcgcgga tcgcacatga tggcgcggcc gatggcaacg
     8341 cgccgctcgt cggtgtaggg cagggccccg gcgatggcac cggccaggtg cgatatgccc
     8401 agccagtcca tcatgcgctc ggcctcggcg atcgcggcgc gccttgtctg gccgaggccc
     8461 acgccggtga cctcaagatt gtccacgacc ggcaagtcgc ggaaaagtcg gccggattga
     8521 aaggtgcgcg ccacgccctt gcgccgcagc ttgtgcgcgg cgacaccgtt gaccgtttcc
     8581 ccttccagtt cgacggtccc ggcgccaacg cgctgaaagc ccgtcaagac gttgatcagt
     8641 gtggtttttc cggcgccgtt ggggccgatc agtccggtga tgcgtccgcg cggcacggcc
     8701 agcgtgacgt cggataaggc cttcaggccc tcgaactgga cgctgacatc gcgagcgacc
     8761 aagtccaagt ccccggaagt cattgagacg ccttttcaaa cgcgaatgag cacgaagcgc
     8821 ttgattttct gcgtcgcagc aagcaattgt aaaattcgaa aaagtgctcg ctgatattgg
     8881 aaattttgat ggctctgaca ttccgtcagg ttcaccactt cgtcgccacg gccgaaaccg
     8941 gcaaggtttc ggccgccgca gccggtctca acgtgacgca atcggccgtc accgcgtcaa
     9001 tcaaggcact tgaagccgag ctcggacaaa aactgttcga ccggcattcg aacggcgtca
     9061 cgctcacatt cgacggccag caattcctgc agcgcgcccg caccatcgag gccagcgttt
     9121 cagatgcgat gcgggggccg catcgctggg gaacccaggt tgatggtacc gtcgacgttg
     9181 ccgtcagcta caccgtggcc ggctacttcc tgccgccgct tctgtcgcgc ttctggcggg
     9241 cctttccggg catcacggtg cgactccacg agttcgagcg cgacgccatc gagcacaacc
     9301 tcatgagcgg cagcgtcgat gccgccgtca tgctcgtctc caacctgcac gaccggcgct
     9361 ccatctgctc gcgccttttg ttgcgctcgc cgcgccgcct gtggacctgc gcaaatcatc
     9421 cgctattgca caagggcggc gttcggctgg ccgatcttgc cggtgagccg tttctgatgc
     9481 tgacggttga cgaggccgaa cgctctgcca tgcgctattg gcaacgtacg ccgcacgtcc
     9541 ccaacgtcat cttccgtaca ctgtccgtgg aagccgttcg cagcatggtg gcgaccggca
     9601 tgggcatcac cattctgtcc gacatggtct atcggccgtg gtctctggaa ggacatcgta
     9661 tcgatctcaa gacgctggat gacgaagttg acaccatgga cgtgggcctt gcctggaaga
     9721 gcaaagcccg gctttccctc gccgcccgcg ctttctgcga gttcgtcgcc ctcgcttatc
     9781 ccggaccgga tccggttcat ttcgattgaa gatccgcgct cggcacgatg gacgggttga
     9841 tcttcacgat ggaaagtatc gagtcctacg gaccggacaa gctggtgaag cattcgaccg
     9901 acagcgtgac gttcggcagt tttgtcatag cctattcgat gaaccgaacg gtttggaacc
     9961 ggcttccgga cgacgtgaag aaggccatcg atgaggcgtc cgaggcaacc gtggcaaaag
    10021 cctgcgccga catcgaggcc gagcaaccag agtctcacaa gagcctggag cgggccgggc
    10081 tgagttttga tccgatatcc gaagacgccg cccgcaagat caaggcgaga ctggcggagg
    10141 tcgcgaaaga atgggcggcg gctctggata gccgcggaaa gccggcgtcg gctgtgctca
    10201 aggaatatga aagcctgctt gccgcgtcac ccccgaatta tccctatgtg gcttgagaac
    10261 ccggatttcg ccgcagggag gcatcgcacg tgatcaggcg ggcaggggat gttctttatg
    10321 cgatcgagcg cacggcgcaa ttccgtggcc ggcatggctg tcggcctcgc cggtgccgtt
    10381 cggctgcagc gtcctggtgc tgcggctcgc cctgcaactc gtcggaaacg tcgtcagcct
    10441 ggcttccaat cgcgacctct atccactgcc gccgattacc ggtgttggcg aagcaaagac
    10501 ttttgaatag cggcggtatg ccccgccatc ctcgtaacac tgaccatcat ggcaacgatc
    10561 tacttcctgg tttggcagga tccatcgcgt gcgccgccca ccgtttcgat ctgctggagc
    10621 cagcgtttcg cgctggtgtg gcaagtcagc ccgatggttg cgttattctc gatcgcgacc
    10681 gggacgatac atctcggggt cgcgaccccg accgcggcat cggcgttcgg cgcattcggt
    10741 gcgttcgtcc tggcgattgc gaaaggcaag atcacgatcg cctcgcttta ccggacgctg
    10801 ctgcgggctt cgcatggcac ctgcatgatc gtcatgattt taatcggcgc gtcgatcttc
    10861 ggatacttct ttacgcttac ccacgtcacg cagggtctgg tcgcctgggt cggcgcgctg
    10921 ccaacctcgc gttgggtgat cattgtactg atcctctgcg gctacatcgt gttgggatcc
    10981 ttcatggacc agcttgccat tctggttctg gcggtgccga tcgtgatccc gttgatcaag
    11041 acgttgggct ttgatccgat ctggttcggc gtgatcaaga tcgtgactgc cgaggtcggc
    11101 atgatcacgc cgccggttgg gctcaattgc tttatcgtcg ccagatacgc caaccggccg
    11161 gttgcggaag tgtttcacgg cacgttcccg catttcatcg cgcatctgat cgcaattgcg
    11221 gtcctcgtcg cattccccgg catcatcctg tggctgccgt ctcacatggg aaattgatgc
    11281 gtgggaaatt gatgcgtcga tccgaagccg tcgcggcttc cctccgcggc gacgtcgtcc
    11341 cctgattaga acctgtcagc cgccctccca ggcctcacga acgcgcgcta tttatttgtc
    11401 tcccggtccc gtgttttcag caatcgcttt cgactatcgg aaatctatct ttgctcaaca
    11461 ggttggatca atggtaggat ttctcaggca agcaccttta ctgaatttcg tgttgctaga
    11521 gggccgtccc gggcggaagc gtaacggtca gcctcacctg aatgatgcag tcgtccgccg
    11581 gtcgcggatg tgtggaacat tgcgagcgcg ccatgcgatg tgcgaagtgc ggggcgacaa
    11641 gccgagaagg caagcgcttt tgcgccgact gcggcgcggc tttggtggcc gtcgttcggt
    11701 ccgagccagc ctcgcgtagc gtcgccggcg cagtcgcccc aaccgaacgc attcagagcg
    11761 agcgacgtca cctgaccgtg ctttttggtg acctggtcaa ctcgacccgt ctcgcggcag
    11821 agtatgatcc cgaagaatgg cgcgacatcg taaccgagtg tctggaggca gccggggatg
    11881 cgatcaagga tctgggcggc tacgtcgccc gatacatggg cgatggtgtg cttgcctatt
    11941 tcggatggcc taccgccagc gaagatgatg cagaacgtgc ggtccgtgcc ggcttgacga
    12001 ttatcaaggc cgtcgcgccg ctaaaccggc ggctgacggg aaaagcaggt tccgagctcg
    12061 cgattcgcgt gggaatccac agtggatggg tggtcattga cgagttgggc accaacaagg
    12121 tcgaggtctt cggcgatacg cccaacatcg cgtcgcgcgt tcaggcaagg tgcgccccca
    12181 attccgtctt gatgaccggt gccgtgcacg atcttgtcgc gggtcagttc atcgtcgagg
    12241 attgtggcgc acatccgctc gcgggcatcg cgcgcgccgt ccagctttac cgggccatcg
    12301 cgccgagcgg catccgtcgg agctggcggc gcgtcggcgt ccgcgggccg accccgtttg
    12361 tcaaccgtca gcgcatgttc gatgtgttgt ggtcgagttg gctcggtgcg cgcaacggcg
    12421 ccgggcgata cgttttcgtg acgggcgagc cgggaattgg caagtcgcga ctggtcgagg
    12481 aattccgctc gagaatcaag gatgcccaca tttggatgga gtgcgccggt gaacgcttct
    12541 ccgagagcac tccgttccat gcggtcatca aattgcttga acaagccttg ggcggggaga
    12601 aagaagaaag cgccgaaggg cggatacgtc agctcaatcg tgcgctgaga ttgagcggac
    12661 tcgattcttc ggaaatggtg cctctcattg cagagatgct gaaacttccg ccgtcacggg
    12721 agtatcctct accccaattc gcgccggagg aggccagggg tcgcctgctg gcgggcctgg
    12781 cggggtgggt gctgaaactt gcgaaacttc agccgctgat cctcgccata gaggatctgc
    12841 actgggtcga tccctccagc atcgaattga tagggatgct gatcgagcag agtgccgacg
    12901 tcccgctgat gctgatggcc acggcgaggc cggagtatca gccgccctgg gaatcgcgag
    12961 cgaccgacaa atcgatcgtc ctcgggcgac tgagcaatga cgagatcgcc gagatgatcg
    13021 ccggttcctc ggatgtcgaa ggtctgaccg aggaggtcgt agccggagtc gtcaaacgct
    13081 ccgatggtgt tccgattttc gcggaagagt tgctgtcgtt tgtattggaa ggcgagggag
    13141 acccgacggc caataacatt cccacgaccc tgctcgattc gctgaccgcg cgcctggacc
    13201 gtcttggacc ggcgcgcagg gtagcgcagg tcgcggccgt actcgggcgc gaattcgact
    13261 atccgctgtt aagggcggtc gtgccgggct cggaccagga cgtacagtcc gcgctcgacg
    13321 cactcatccg ggcggatttg atttacacgc aaggcaggcc gcccagagcg acctaccaat
    13381 tcaagcatgc attgatccgc gacgctgcct acgaaggctt gctcaagacc gaacgccgcg
    13441 agctccatgc ccgcgttgcg cgcacgatag gtgagcaatt tccggcgtcg gctgcggcgc
    13501 agccggtgct gctggcccgg cattggactg aagcagccga agccgagcca gccatcgccg
    13561 cctggaagaa tgcggggcag gcggcgcttg cgcggtgcgc gttcaaggaa gcggaggagg
    13621 cctatcgtca ggccagcgcg ttgctcaatc ggctgccgcc gtcggagacg cgcgacaggg
    13681 atgaactgga cctttgcagc gcgctcgtgc gcgtgctcca ggtgaccaag ggctattccg
    13741 cgccggagac gatgcagctt ggggcgcgtg cacgggtgct cgcggaacgg ctcggcgacc
    13801 tttcgcagct ctttcgcctg cgcgcccgaa cctgggcctc catcttcttc acaggcgact
    13861 acgcggccgc cgcctccctt gccgagcaaa tcctcgaaat tgctttgacc gaaagctcca
    13921 attccagcca cctgtttttc gcacactacg cccaggtgca ggcgcgattc tatgcagggg
    13981 accttgccgg agttgaagat cactttgcga agctctctcc gttgctcgac accaggtcga
    14041 ttgccgctgc agccgacctc atccttccga tcggggtcgc gagccgtgcc gcctggcagc
    14101 agggccgtgt cgatgtcgcg cgcgagcgga tgtctcgcgc gatgaatctg gccaggaaga
    14161 gtcaggaccc ctatgccatc gcgatggcgc tgcacttcga gggtaatctg cactggtgcc
    14221 tcaaggcgcc gcggcgggtc gaggtgattg ggaatcggct gttgtccctg tcggaggaac
    14281 atgggctcag ctatgcctcg gacctggcgc gcgtgctgct tggctgggcc aaatcagaac
    14341 tcggtcacgc cgctgaaggc gtggaattga tcaaccaggc cttggccgga ttcgccgcgg
    14401 caggcgcgaa agtggctatc acctattttc tcacgctcct ggcgcaggcg caggcgtgtg
    14461 ccggcgacgc tgaatcaggc ctccgcacca tcgaggaagc cctcgccgcg aatcctcagg
    14521 aattgatttg gcgcgcatat accctgacat gccgcgggga gctgcggttc aaactggggc
    14581 agccggcaat ggccgaggcc gatttccgcg acgcgatcga aatggcgcga tcactcgagc
    14641 acaaagcctg gcagctccgt gctgcgacaa gccttgcacg gttgctgatg cgacgtggcg
    14701 atcccctcgc cgcgcgggga agtctcgtgc ccgtctattc cagcttcggc gaaggtttcg
    14761 agacgcccga cctgcgggaa gccagattgc tcctggagga gatggcgcat ctacccgcga
    14821 cctgacccag agcgcgatga cttttcttcg aatcgtcatc gcgctctatc cttttgtttg
    14881 agcatgatct ccgcgcaaac gcattccgcg tttgtcgcga gggaaaaccg gtaaccaccc
    14941 tcgggtcaag cccgagggca tgcttttccg gatcatgccc taacggcgcg acagctccct
    15001 ggtatgatcg acaagcgcgt aaagcggcgc cggattggcg gcatgcaaat gcgcctccca
    15061 cttggcctcg gccgctaatt ggtcgagccg gtcctctgcg gccggtaccg ccgccttgac
    15121 cgcccgagag atttgcgcca gctcgtcatc cgatcgcacc ggcttgcaga acagttctgc
    15181 gatcaccggg tctttgatgg caatcgaggc gggcagcgtg aggatcccat ccctcagatc
    15241 ctcttcgccg ccgccgccga gcgattccag tcccttgaca tcgcccacgt cgtcgcaccc
    15301 gtgatatagc acgccgagca gcccgccgaa tttgcgaagt agatcgtccc tggtggcgac
    15361 acatgcacag gtctcgaaca tcgaccctgt atcctcgccg gcgatgttca gccagtcctc
    15421 gacactgagc agctggcgtc gcacgttcca ttgcaggcat tcggccgccc cgagacgctt
    15481 gatcagatcg gacatcagac ggatatcgat ccggtctccg gccgtcatcc ggaagccgtc
    15541 agccacgata tagccggacg ccatgagggc ttgcagaagc ccaaactggc agtgcagggt
    15601 cggtctgcct cgccgcgttt cggatccatc gacgatgtcg tcgatgatca gcgtcatgtt
    15661 gtgcatcatc tcgatcacgg ccgcggaata gatcagtcga ttcggaaccg gctcgtctgt
    15721 cgtggcccga tagacgctga agacagtgag gggtctgaaa tatttggaac tcgtgatgaa
    15781 ctgccattcg agcaggggct tgatctgctc gttggcgccg cgaatccagc tgtcaaggat
    15841 ttcgcgaagt tgctgcatct cctccgtgaa gccgagctgc gctatgatac tttctgtcgt
    15901 tgcctttgac atcgcgactt ccccaagtcc cctcgaccgc cgcccacgcc cccgtgggag
    15961 ccagtcgtcc ctgtcaggtc aaagcttcgc gcgatgcctg cgaacatatt caagccttgg
    16021 ccggtggcgt cctgagccgc gcagcagccc gctgccctcg gcaagtgcgg atcgcagagt
    16081 agggccagcc cccccaggcc gcgccgctgg aggttcgctc gcggcggtac tcactacctt
    16141 caacagatct gtcagcaggg aggcaagacc ccgtggtttg tcggccgatc tcttggaggc
    16201 gagattgcac aaatgatcgc ttgccgccag cataccccgg gcggttgccc gcaatccctg
    16261 ctgaaccgtg gagcttgaat ggtcgggttc gccagcgggg gaaagcaaag caactcccag
    16321 agtctcgagg ctcgccaccc aatcggctac gtatctcaga gggatcaacg cgagtgtcgt
    16381 cacgggtgcc agggcccctt ccggtgtctc ggcctgtccg ttcgccagat cacgcagcgc
    16441 aacattcacc gctgcccagg ccgtggcacc ataggcgagt ggaagcaatg cgagcttcgc
    16501 gaccgagaat tccgcggagc tgggaatgac gcctggctcg accgtgatat cggatttgcc
    16561 ctctgctttc atctgcaccg cacgcgccgc gtggagcccg ctcaagacag ccgattcgac
    16621 cgtcgccatc ttcacttcgt tgcgacagaa atcgccggcg aagaacacgt tccgcagatc
    16681 gggatagctt ggctcaagca gcacttgctc ggaattcatg tcgttaagga agagcgtgtg
    16741 ggcacggttg ttttgaggaa acgacttcga ataatcaata ttgctgtcgg cgtctcccca
    16801 ccggcgaccg ggcttcaccg cgggcaggta gccggccagt tcctgtatca tcagatgcgc
    16861 ccattcctct tcattcgcgg acgggtacgc ataggcgtca gaggcggcca ggatcagaac
    16921 ggagtgctgc tccttgatgt tgctcaagct ggtccagagt tgtgaaatgt cgatgaacgt
    16981 caggtaaccc cgcgaccggg ccagtccgac atgctcacgg ggtacgttcg gaagcttctc
    17041 cttgaagaat acggtaacga ccaggatact tgccgtgcaa aggcgttgca gttccgagag
    17101 tgccggaagg cgctcgacga cacaccgtcc agggcggcca ctcatcacga gctttgccag
    17161 ttcgggggcg ggcaccgcaa agacgacatt ggcatgcgtt gccttcgtct tgtcgctcag
    17221 tgtgagctgc aacttgtcgt catcgccgac ctcgacggcg gtgaccttaa cggacgccct
    17281 gatattgcaa tcgttcttca ggctctcctg ccagggcttg atcagcatct cctcgaggct
    17341 tccccgtaac agccaggcga agggcaagcc gctgctgccg agcacgtgct tgacgaagtc
    17401 cttgtaagcc gcagcagatg tgtcgctgct tgagatgcac cagacttcat tcagaatcgt
    17461 gttgtgcaga tcggcgcagt ccaacgtggc gtagggacgc gagtacagga acccgttcac
    17521 cgtctgctgc tccagaagat tcgaccggtg aaacggctgc gaggccaggt ccagcagggt
    17581 gaagccgacg aggaacatgt cgggtggcgg caatgctccc gaccggaggt tgtccaaggc
    17641 gttcttcagc gtgttggggt tcttcaactc cgtgtagcgt ggtgtgttgt ccgccgagtg
    17701 ctgcgggcta ctcggaggat caagaagctt gacgctcatt cgcggctcga aagcgtcatg
    17761 tcgcttgatc ttgagatcgt cctcgacgat gcgccagaaa ttcacatacc agtcgcagaa
    17821 cagatgggta tagacgtcgt ggtacatgcc gttgctgagt ttggaggaga ggtcgcctcc
    17881 cagcatccca tccttctcat acagcgtcac cttgtacccg cgcttcgaca ggcgtagtgc
    17941 gcaggtaagc cccgcgattc ccgcgcctat gatcgcgacc tccttttcgg ctggcatcgg
    18001 cggtttggat gttttggatt gagatgcggt agaggtcacg cgcgtcctcc tcgaaaaatc
    18061 tgatggtcag atcaacaatc cccggaccct ggggttctct tgagtcagca aggatggccc
    18121 gagctgcacg gcttttttca cgacatcggc cagccaggct gcgtgccagt ccgggtccgc
    18181 gctgacggat cgtttccatg cgctctgcca cgcgacaagg tccccaagca ttgcatcccc
    18241 gccgatcggt gttccgatga cgaagcttcg aagggtgttc atcgtgccct cgcccaggta
    18301 gcgcaggatg ctcgctccga tctgtcgcag gattcccaag gggtggacgt tcaaccctcc
    18361 gaagcctgcc ccggcgatac gggcaaaatc cccgtcggcc atcgcccgtt cgcagccgcg
    18421 cagcgccgac ctcatccaga attcacggaa gtgccaattg cgcatcgccg cggcgcagaa
    18481 acccaggaag agcatgccgg ggtcgaaata gctccggaaa tcccgctcga aattcgccag
    18541 gaatgcggcg tcaaatcttc cgagttcgag ggccttcagc aaggtcgatg aggcaatcaa
    18601 ggctgactcc atgccttgag tgattccttc gcccgtcatc ggatcagcga acgagccggc
    18661 atcgccaatc aggaccccgc cgtcgaaata gttcggaccc acaccgccat acatcttgac
    18721 gacaccaccg atcgctttac tcaccacctt gatccgcgcg cagccgggat gccggatctt
    18781 gagcctctcg atcgtcgcca cgaaatcctt cggcaccgaa tggccgtgcc ggtgggaggt
    18841 ttcgctcagg ataccgattc cgacattcgc cctgccaccg gacataggga acatccagcc
    18901 atatcctgga acgacgtctt cgtcgaacca gacggtcgcc tcgccatcct gcaccgaaac
    18961 gccttcgaca taggcccgct gggcaatcga gatgtgagcg cggtcggtca tttcgaggtt
    19021 gaacgacctc gcaacgagag actccgcgcc atccgcacct acgatcagcc gagatctgag
    19081 tgagaagtcg ctttcgcggc cacgcacatc gacgtgaatc caaccatctt cgcgcctgat
    19141 cccgcgcgca gcgcagccgt catatacctt ggccgagacc gctcgggccc gctccagaag
    19201 atggtggtcg agaatatggc gcggcacgac aaagccatga ggcggcagcc catgctcggc
    19261 ctgatagtaa gggatgacgc cgcgaaagcc gaggcgggga ccaaagtaaa tgcgggtgga
    19321 tgttatcggc aaaggcgatg acgcctgcag tgcctctcga acgcccatgg cctccaggat
    19381 tcgcaggcct gcgggctcga cgaaatcgcc gcagaccttt tcgcggggaa agatggcgcg
    19441 ctcgataacg gccacacgca caccgcgccg agccagcgac cacgccatcg tggagcccgc
    19501 tggaccgcct ccaacgacga tcacgtcata aaggtcagaa acagacactc gaaaattcct
    19561 aaacgcatgc gatcagaatt tcagagcaaa gggggggacg tcaagccata gatacacacg
    19621 agtccgaatt cggcggagac gaaaacgagg ccggaaacgg cgtgctgaac cgctcatctg
    19681 gtgcttcatg gcacagaaac ttctcttacc ctctcggtcg cagtcgcttc atgagcagca
    19741 cgcgaccaag tctctcgaag ctgcgcgcac ggccgaattt gttgaaccgg aaagattaag
    19801 tgcacagctc gggcgcatcg cgcggcgagc gcggagtcgt atgtggactt gagtttgacg
    19861 tcgttgcgga ggggaagcaa cctagactcc gtcattccgg ggcgatgcga agcatcgaac
    19921 ccggaatctc gagattcccc gatgggcatt gcgcatctga ggtctggtcc tgcggaccat
    19981 cccggaatga cgggtgtgtt acaacggctg ctgccttcgc cgcggcgctt cggccgacgc
    20041 gaccttgaca tcccctgctt cagcttcgtg gcgccagctc aaaatctcca ccgccagcac
    20101 cggatgatta aaccccttca ggttcagatc gtcgagcggt ttgccttcga cccagggctc
    20161 gaccatgcca aagacgcgcc gggagaccac gatctggttc gcttgcgcct cgtcgcacag
    20221 ccgcgaggcg aggttggtga cgctgccgat cgcggcatat tcgagccgct gctcgaagcc
    20281 gatctggccg agtgtggcat agccgagcgc gatgccgaca ccaaatccca gactatgtcc
    20341 gcggttgcgc cacttctggg tcaacagccc gatggtgtcg cgcatctcca ccgccatctt
    20401 caccgcgcgc ctcgtgtgat cggcaaattc gatcggcgcg ttgaacagga tcatcacgcc
    20461 atcgccggca tagcggtcga gcgtgccctc gtagcggaaa atcaattcgc ccagggcggc
    20521 gtgatattcg cgcagcacgg ccatcgcctc ttccggctcg gtgctctcgg tgaacgcggt
    20581 aaagccgcgc aggtcgcaga acaccaccgt gacttcgcga cggtggctgt cgagcaagga
    20641 gtcgtgaccg tcggaggagg cgatcagttg cgcgacctgc ggcgccagaa aacgttcgag
    20701 cttgcggatg cgctcgattt cgccgagctg cttttcgacg cgctcttcca gcgatttgtt
    20761 ccagttcagg agctggtcgg cctgctgctg cacggtggaa tgcgcggtgg ccaattcgcg
    20821 gcccttctgg tcgacctcgg tgaacagccg cgcgttgcgc atcgccagca ccgattgatg
    20881 cgcgaaggtc ctcatcagcc cgatcagatt ttcggaaaac tcgccggcgc tgttgcgcag
    20941 caccaccagc gatcccaaaa tgcctttctg gtcgaccagc ggcaccacca gcaccgaatg
    21001 aaatccggcc gcgatcgcgg cgtcgcgcaa tggatggtcg gcggcgtcag ccaggttcgg
    21061 gatcgcaacc ggctcgccgg tggcggcggc gtcaccgagt acgctgccgt cggcgtcgat
    21121 cgcgagccgc gcctgttcgg tcgatttatc gatcccgatc gcttcggtca ggttgaactg
    21181 atgtcttgcg gcgtcatagc caaaaatcag caccgcatcg gcatgggtga tttccaacgc
    21241 gcgggccgcg accgtcggca gcacggcatt gaggtcgagc gaggaggcga ccgcgcggcc
    21301 gacttcttcg agcaccttca gttcattgac cgactgcgcg agatcgcggg tgcgctcttc
    21361 caccttggat tccaggcccg aataagtctc gtgcaacttc ccggccatcg agttgaactg
    21421 atcggcgagt tcttccagct cgtccttggt gtgcacgtcg atgcgatggc tgaaatcgcc
    21481 ggcgccgagc cgccttgcgc cggtgcgcag cgcggtgatc ggcaccagca tccgccgcgc
    21541 cagcacggtg ccggcgagaa tcgcgacccc aagcccgagc gagatcaaca gcgcgatccg
    21601 caccagctgg tcgcggatcg gcgccaacgc ctgcgcggtc ggctgctcga agcacacgaa
    21661 ccagccgagt tttggaatgg cgttcgacgc ggtcagcacc gcatggcggt cgacgtcggt
    21721 gcccgatgcc agcgcctggc cgttcggtcg gatcaaggcg gcgacctgcg gcaacttcga
    21781 cagatccttg gccacgtcag ggcctctggc ggagctcgcc agcacctggc ctttcgggtc
    21841 gatcacatag gcgaaagcgg tcttgccgac ctgggcgtca ccgaaaaaat ccgacagcag
    21901 gccgaggtcg atttcggcca ccgtgaaggc ggcggcgggg tcggagcccg aatgcgagac
    21961 cgcgatcgac atcagcgggc gcgagcctct gaaaaaggcc ggcgcgtcgc tgacgccccg
    22021 ggcgacggtt tcggccatct gcacgtcgcg ggaaagatcg gtgccgctgc cgaccgcgat
    22081 cgcggtgcgc gatagccgca gctgctcgcg gccgttctgg tcgagcaagg tgagctggct
    22141 aacggcgggc acctggttca ggagctgcac atagtcggcg tggtgatcct ccagcgtcac
    22201 tacgccggaa taggtcttgg cgcttgatcg cgtcacccag ctgatctgcc gctccagctc
    22261 ggagatcgac tgctcgatcc gccgcgccgt cgcctcggcc ttctcgctca tcgcgtcgat
    22321 cagcgtggtc ttggtggcgc ggtagctgat ccaggtctcc gtcgcgccgt tcaccgccag
    22381 cacgaatacc acgaggccca ccagcgacat gacgtatttg gcgaacaggc cttcgcggag
    22441 gaaccagatc ttgtcattca ccccgatcat ccagaccctc gtacgcgccg ccttcgaggc
    22501 gtttcgcatc cgttgcccat cggcggaaca gcataggacc agccggaagt ctgtttagca
    22561 caattcgagg gcccggaccg ggcctctgcc agcgtggtta gccgcgattt tgggcagttt
    22621 tgggggtgag ggttgcggcc actagcctat cgctccctcc tccttatggg ggaggaggga
    22681 gcggggcaac ggggattgaa gaccgtggtt agccggacct caccgattga aaagctgctt
    22741 cagcacgtcg ttcatgggct ggctgtcctg ggatacttcc ggggccggat cgatttgcgc
    22801 cgctgcaggc ggtgcgtctg gacggggaga ctcgggcgcc gcgagcgtgc ggctttggct
    22861 gcggcgctgg ccgaggccct gctgcagcag gttgccgagg gtcgagccga gctggtcggc
    22921 cagcccattg cttcccgatc cgctgccgcc gctcgatccg cttccaccgg atccgcccaa
    22981 tccgcccagc acacttccca atccgccact gctgccgaac aggcccttgc ccatttcctt
    23041 gagcttggcg taggccgcat cgggattgtc gaggatgccg gccatgtcgg gatagatccg
    23101 cggctcggcc caggggccgt cgatcatcac ggggatgccg agcccgaccg gatcggaggt
    23161 gcggccctgg ccttcggtgg tcagcacgag cttcggttcg acccgaaacg ccagcgcttt
    23221 cgcggcgaga tcgatggtgc cggccccggt gatcttcacc agcgggccga ccagattgag
    23281 atcggcggtg gtcgcctggc ccttgtcgat ccggaacgag gccgacagct gcgtgaggtc
    23341 ggtggtctgc tccttgccct cctgccatcc cgacagcgtg cccgaggtga gcgagcggat
    23401 catcgcggcg acgttgatgc ctctgatcgc gccatcctgg aacacggcga agaccgttcc
    23461 gctcaggttc gacatgacca ggcgctggct gttgccggcg gagcgcaacg tcattttcgc
    23521 ctgcatcttg ccgtcgagct tgtcgaagtc ggccgcactc cgcagcagcg gcaaggcgcg
    23581 gacgccgacg agatcgccgg agaacgcata gccggggctg ccgctggtca catcgatgat
    23641 gagctggccg gaagcctggc cgccatagac gccgagattc gaaaagctgg ttttgagcac
    23701 gccggcggcg accgccgtat cgatcgcgac cggcgcgaaa cgcgcctcgc cgatgttgag
    23761 ttcggcagcg gaaacccgca attgcgcgtc gacatagttc aggccggtga ggtcgatcga
    23821 ggcattgctc cagggctgcg aacccagcga ggcggatgtg gcgcgctcct tcgacgtcgc
    23881 gacttcaagc cgctgaaaat cgagatcgag ttttaccagt ggcttgctgg agagatcgac
    23941 cgatgcccag ccgttgaagg cgccgtcgcc gagcgttccg gtgacgccgt tgatcatcac
    24001 gatcgaaccg ttgagccgga cctcggcctt ggccgcaagc ggggcctgca acaggccgga
    24061 tgcatcgagc gtcagctcga ccggaatgtt ctggcgctgc agcggcgggg cgggcaccgt
    24121 cgccgttatc gcgaatttga gcggatgctc gctggcgcgc gcgcggccgg tgaccttgat
    24181 cttgcgatcg tcgccgatca aggcttcggc gttgatgcct tcgatgcgat tttcgacgcg
    24241 gtcgcgcagg ttgaacatca ccatcgtgcc gtcgacaatc gtcacccgat caatcgcgag
    24301 cgcattcgca tccttgccgt cagaggacgc cgccggaccg gcagccggag ccggcagccg
    24361 ctcgcgttcg cgcagcagcg ggtggtgcag cactgggcgg gtgatgacga gttcggtgat
    24421 ttgcggatgg cccgaccaca cgcttttgag cgtcatgtcg gcctgcacgc tgccgaccgt
    24481 cacgcggttg gaggcgtcgc gatccttcgg cgcctcgagc gtgacgtcat tgacggtcac
    24541 gttcagcgac ggccacagcc cgattttggt cgagcccgcg atcgtcagcc gatacccggt
    24601 ttcgcgctcg acccggtcct ggatcgccga ggtcaggaaa cccgagggga tgccgacgat
    24661 cagcagcacc gcgacgatga cgataacggc agcaacggcg gcgccggcga ttttcagtgc
    24721 tttcatgacg atatattcca aaccgggcgg cgtctgacct ggccccgcca tatgcggggc
    24781 gtggcggatt gcccaagtcg accccggaaa caagtctacc ccggaaaccg gactggccca
    24841 aagccgccaa aaataatcta gagttgtcgc aaagctctgt gacttatgac acacttcgaa
    24901 ccgaagcgat ttttacgaaa tataaagaca ctgccgattt tgacggtttc taactagcgc
    24961 ggaaggcatt gccatgagta aagcgaccga atttgcggtc attctgaaga tgaatccgat
    25021 gttcgcggac ttgggtaccg acgaattagc gcgaatatcc ggcctttgcc acacccagca
    25081 acttgggctg ggcgaagtgc tgttccagaa aggcgacagc ggcgacgcgc tgttcggggt
    25141 tcgccgcggc cagatccgga tcgaaaccgg cgccgccgac ggcagccgcc tgaccctgaa
    25201 tttcatgggc ccgggcgatc tgttcggcga ggtcgcggtg ctcgacggcc agagccgcac
    25261 cgcggacgcc accgccggcg agccgaccga actgttcgtg ttgaggcgcg aggattttct
    25321 cagccatctc gagcgtgaac cgcgcgttgc gatcaaaatc atccagctgt tgtgtcagcg
    25381 gattcgctgg caaagcgagc ggatggagga atcggtgctg cagccgctgc cggtgcgtct
    25441 ggcgcgacgg ctctgcgcgc tggctaccga ttacggatcc gaagtgcaca tctcgcagga
    25501 gcagctcggc gtcttcgtcg gcgccgcacg cgaaagtgtc aaccgtcagt tgcagctctg
    25561 gcgcaaggaa gacattctcg atctgcagcg cggccgcatc ctgctgctga acatgaaccg
    25621 gctcaccgcg gtggcgcgta acgagtagca aagccgcacc acatcgttgc cctgattttg
    25681 ctgcgcttcg ccttgcaatt gtgggccaac tgcggcgcgc gaacatcttt cagctcgcag
    25741 cgcgctcgaa catgacctca attgagccac caatattttg aatttgggac attaagattt
    25801 tttaacaaat ctcaacacgc agcctcgcca tgcaaaacgg gttgccgcca tcgcacgccg
    25861 tgcgacgggg atcggttctg tctgtctgct gcacgctatc agtgtgagtt ggggtgccca
    25921 tggatcttgc attctcgaat atggctgcgc gctcgaagac ttccccggcg cggtggcgaa
    25981 ggccgtttct gagctggatg gatggcatcg aagccggctg ggcgattccg cttttgctga
    26041 tcggattcac cgtggtctgg ctggcgtttc tgatcatcgc ctatctcgac ggcgatctgc
    26101 attacgatgt gctggaaaca tggacgcttg gacgcggcat cgattggagc tactccaaac
    26161 atccgccgct gatgggatgg gtggcacgcg cgtggacatc ggtgtttccg ctgacaaact
    26221 ggtcgtttca gttgttggcg ctgaccaatg cggcattcgc gctgtggacg gttgatctga
    26281 tttcgcgccg cttcgtcagg ggcgacaaga gaatgatcgt gctgctgctg ctgatgctgc
    26341 tgccgatcta ccagtttcac gcccagcgct tcaacgccaa cgcggttctg ctggcggctt
    26401 ggccgctcgc cacctattgc ttcctgcgct cgttcgaaac gcgcgagatc cgctgggcgg
    26461 cggccgccgg cgccaccgcg gcgctggcga tgctcggtaa atactactcg gtgtttctga
    26521 tcggaagctt cgtgttcgcc gcgatctgtc atccgcaacg ccgcgcttat ttcacctcct
    26581 gggcgccctg ggtttccctg gtcaccctat tcaccgcgct gctgccgcat ctgaattggc
    26641 tgctggtgaa cggcgcgccg ccggtcgttt atgcgctgac gcggcatacc ggcaaggact
    26701 ttgcccctgc gctgctcgaa tcggtggtct ttctcctcgg cgttgccatg atcctggcga
    26761 taccggcggc gacatgggcg ctgatggcgc aagaccgcat caagaatttt tccgaggatt
    26821 tttgggcgat gcattcgggc ctgttgctgc tgtttcttgt cagcatcggg accatcgcgt
    26881 ttccggcgat cacgacggtc gcgctcggag ccgacatgcc gccgctatgg gccttgcagg
    26941 gacttttcct gttcgtcatt ctgattgtct gcggcgcaag ctatccgatc gaacggtttt
    27001 attcggtcaa tctgacggtt gccgtcatcg gcattgcggt ggtggcggtc gtcgtggcgg
    27061 cgccgctgca cgccttctat cggaacggcc atccattgca cgagggccgc aatttcttct
    27121 cgcaggcggc cgcggagttg acacggcgat ggcacgagca gtccgatgcg gcgcttgccg
    27181 cggtcggcgg cgacgatggc ctcgccctcg ccacggcttt ctacagcccg gatcatccgg
    27241 tctatcaatc gccgcttgtc tacccgcata cggaaacgtc gccgcgcgag gcgagcttca
    27301 gccgcggctg ggcagccttg tgctatgacg gcgacaccag ctgtatcgac tcgatggaaa
    27361 ggaccgccgc ccgggccggc cggtttgtcc gaacccggtt cgtcgttcag tcgacggtgt
    27421 tcggtcagcc cggcgccagc cagggcttcg cggccctgat ggtgccgcct tacgatgagg
    27481 acaagattac gccgccatcg gcgtcgtcgg gtccggcgat cgcgcaaaat ggttcggaag
    27541 gcgcgtccgt tctggttcgg caggacgagc cgacatgctg cacacggaat ccgcgcctcg
    27601 acgccgcgga gcgcctgtcg gagctctctc ttccagagat ttccgccagg gacagcgaca
    27661 ggaagcgcgc ccggcatgct gtcgcaccgg ccgccgcggc gtaccaggcg aattggccgg
    27721 atcgcccggc atcatccgcc acgcgcgacg gctttgcgcg ctggccgatc ccgacgccgc
    27781 cgccgcgcgc gaaatcgtcc gtcgcaagaa gcagctttgg cgcaagaccg gccttgcagg
    27841 cctgcagcac ccgcggaccg tccgtttcgc ccggtcgcga gcggatggcg gactcgacgt
    27901 cggagccgct gtccgtccgt ttccgacgtc ggttctgcgc gatggccgcc gatttcgacg
    27961 aacggatgcg cgtgtcgcag gagaagttcg atgccttcgt cggcaatgcg cgcgaaagcg
    28021 ccgaccgcag gctgcagctc tggcgcaggg actccatgct ggatcagcag cgccggggga
    28081 tctagtcgcc gaagcgcttg aggtacgggg gcacggcgcg aaagcgcctt gcccacccct
    28141 gcaagtcaag cctgcaagtc aagcttcggt cagtcgcgat cgcgcggatg cggccgaggc
    28201 agctcgtgcg cggatctcca gcttttgacc cacctcgtga ggtcgacaac cgaatcgagc
    28261 atcgccatga cttcagttag tgcggcggca acctcgtgcc cgcgctccgc cgccatctcg
    28321 cgaaggcgag cgccggtatc atcatcgatc tcaagcgtgc ggctgacctg gttcatggtc
    28381 caagtttagc atcctgagag cgcgtgtcga ggtgcaagct attgacggat tatttaggtt
    28441 aacgccgcgt aaggcgatgc cgttcgtagc tgtgttgatc aagcgcgcgg cggatcgggt
    28501 tcatgagcaa agtttcatct gcggcggtct gtcgagtcgt tgaggggtag cgtcatgccc
    28561 cgcgaaggcg ggagatccgg tacgccgcag ccttgcgaat ccatcattgc cgtcggcgat
    28621 tactggatca tccgctttcg cggacgatga cgtctgtgaa tgtggaagag tccatgcgag
    28681 cccattggaa ggccgcccca tcactccgcc gcgggcgaat gcacgatggt cggcggtccc
    28741 ttgtgctcgg cggcgcgctc cggctcatgc ccgtccgcgg catgttcgct ctcggcgtgg
    28801 gaggccagca gcttcttgcc aaagcgccag aacagcgcgc cgacgtcgtc catcatcatg
    28861 aacatcgccg gcacgaacac cagcgacagc acggtggaga ataccagacc gccgatcacg
    28921 gcgagcgcca tcggcgagcg gaattcgccg ccggcgccga atgccagggc tgaaggcatc
    28981 atgccggcgg ccatggcgat cgtggtcatg acgatcgggc gcgcgcgttt catgccggcg
    29041 tcgatgatgg ctacatcgcg ccgcttgcct tcgcggatcg attccaccgc gaattccacc
    29101 agcatgatgg cgtttttcgt cacgatgccc atcagcatca aaatgccgat ccacaccggc
    29161 gtcgtcagct gcttgccggt caacagcagc gccacgatgg cgccgccgat cgagagcggc
    29221 agcgaaaaca aaatggtgat cggctgcagg aaggtgccga acaacagcac cagcaccgca
    29281 tagaccatca tcaatccggc tgttatcgcg gtggcaaaac cgtccgacag ttcggcaagg
    29341 ctttcggcgt cgcccgaggg gctcaccttg acgcccttcg gcaggctctt catgaccggc
    29401 aaatcgtaga tttttttggt ggcgtcgccg agcgccgagg agccgacgag gtcggccgcc
    29461 accgtggcct gacgttcccg gtcgtagcgg ttgatgctgg tcggaccctg gtcgagcttg
    29521 atgtcggcga caacggacag cggcacgccg ccgcgttcgc cgtgctggcc gagcggcacc
    29581 cgcaactgct ccagcaattg caggtcgccg cgcgcgccgt cctcgagctg cacccggatc
    29641 ggcacctggc ggtcgccggc atcgaacttt gcaagggccg ggccgacgtc gccgatcgtt
    29701 gccacacgaa tggtctgcga aaggctctcg gtggagacgc cgagccgtgc ggcgagatcc
    29761 gcgcgcggct ggatccgcag ctcgggccgg tcgagcgag
//
LOCUS       DATNOI010000134        21047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_308920,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000134 DATNOI010000000
VERSION     DATNOI010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 21047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21047
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308920"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="VN968_05020"
     CDS             complement(<1..159)
                     /locus_tag="VN968_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXL28634.1"
                     /translation="MTSIPISNIRNFAIVAHIDHGKSTLADRLIQTTGGLQAREMKEQ
                     VLDSMDIER"
     gene            274..1899
                     /locus_tag="VN968_05025"
     CDS             274..1899
                     /locus_tag="VN968_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXL28635.1"
                     /translation="MSTASFLPAASRVRSRLSVRRMAAWLAACASDPKAAPRLVIGFA
                     AAHAVLWTLILVNLKAAQDVHMDVAEAFAWGQKFQFGYGKHPPLAGWVAGVWFRIFPV
                     ANWATYALAMATLGCGLAICWLIALRAVDRRRAFFVVVMLALYPIFNFKGFKYNPDLL
                     QLVTLPLVVLAFLHAFEKRSWRSGLWLGLAGALALMTKYWVLTMIGAIGIAALIHPGR
                     WQFLRSPAPWVAIATLVVAMVPHLIWLKQVDFVPLTYAGDVYGLSSRAQSTQLVLGYV
                     GHNLALLAAPVVLAALAMAWSPRWWMTLARQPLALFTRAWSRGENTGVNASQALNIWI
                     IQIVVAVGPPLGGLIFTVYMKTDWGISLFFLTPLALIAMPSLRLHRMALFHIAAIWLV
                     ITLAVLAVSPTIAARELAENPNGASGYGARSELARELTEAWQTRFHTRWAVVAGTTEI
                     GEPMTFYSPDHPAPFTPGEVWSSGLTSLEEARRLGFIGICDTSDPRLPVCDAWMAANA
                     DRAERLAITTQRFFHGHPGPAISWKVYVVPPAQ"
     gene            2225..4201
                     /locus_tag="VN968_05030"
     CDS             2225..4201
                     /locus_tag="VN968_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28636.1"
                     /translation="MIGLAISLVAGALIRTGRAADARTFINEAADLFNPENRVYVRGS
                     TFGVLVGAFPFLFVPSIVASIALGDGKSAVEAVVRFCQSMQKSELTASCQRRTRQDDW
                     FESNPMVRDLLNHHAIEPYGPFCFDHRWMLERQEEILRRGPLDRRLEATYEFNELLLE
                     NALVIEQPARGLALIEDGFNSLGPDSSGPVSGHLAFNAVCVLAALGRFDEALAAAYAM
                     ARHGYGLLARFDLAAVAERMPWWTALMRQNNWLGPLAATEAYQSFLQNEVASRPFDVS
                     DPAFNPLCAVRDGALGGKAKQRCWLSREPISPGEPVVRIRRLFGHASDGEFDIIKKDA
                     FAASGWATARQEFETDTVPVRLLFPDTRTHAEGSRIWRTAAIFAFHYDVARNPDAFDL
                     DRAVALIADRGPNPIRFEWVRGKDDVAPAFDPMVNDVGHGDAVNFAWRLLKAGCGRDI
                     FRRVANLPQEKADKVFAMLATFDRNDCRGASAEYFGLPDLPKMMERAFRKRPSLEDHI
                     AAADFASAQPRFRRGLTAAMRAYALHLYSNNRPAADWFLQGLEHFSGARCCRLLTFLI
                     HHPEDDWVLATMIERGWLPQRFGRDDAYSNALSFYYRTVLFNRMLHAPEQLENWLAPG
                     WPGSRMTRSKDRETVRLVEQWRKKPRRSHGRPSR"
     gene            complement(4249..4578)
                     /locus_tag="VN968_05035"
     CDS             complement(4249..4578)
                     /locus_tag="VN968_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HXL28637.1"
                     /translation="MTDDDASPEIDHGPSVPSGAVSREIPIINKRGLHARASAKFVQT
                     VERFNAEVWVTKGNETVGGTSIMGLMMLSAGPGTSIVVSAIGPEAQAAVDAITELVAS
                     KFNEESA"
     gene            complement(4575..4976)
                     /locus_tag="VN968_05040"
     CDS             complement(4575..4976)
                     /locus_tag="VN968_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HXL28638.1"
                     /translation="MIGLVLVTHGRLADEFKAALEHVMGPQKQIEAVTIGAEDDSDLC
                     RSDIIEAVNRVDSGDGVAILTDMFGGTPSNLAISCMSRPKVEVLAGINLPMLVKLAKV
                     REERSLPDAIAMAQEAGRKYVTIASRVLAGK"
     gene            complement(5110..5637)
                     /locus_tag="VN968_05045"
     CDS             complement(5110..5637)
                     /locus_tag="VN968_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HXL28639.1"
                     /translation="MTDAARASVHASAVLVGNRAVLIRGPSGAGKSRLAFDLVLAGRS
                     GQIPEAVLVGDDRVHLDTHDGEIVVRPARELAGLIEIRGLGIRRCEFTGAAIVGLVVD
                     LCAADAERLPPPETLLARIFGVKIPRIPVGEGYAALPLVVAALTTTGSSTSTQPSTNC
                     LEGIGNHMRHTIATE"
     gene            complement(5634..7451)
                     /locus_tag="VN968_05050"
     CDS             complement(5634..7451)
                     /locus_tag="VN968_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXL28640.1"
                     /translation="MLDRTQPDSSLSAEDASSSLVLDGANFENPSAQGWQRPLGWLRR
                     AGQFFFALSFSSLTRRIVSLNLAGLVALVASILYLSQFRAGLIDARAQSLQISAEIIA
                     GAIAASATVDTNSITIDPDRLLDLKPGETYGAPDEYSGLDFPINPERVAPVLRRLISP
                     TKTRARIFDRDGGLILDSRNLYGRGDVLRFELPPPTVEKPGIAERTMIAVRTWLNRGD
                     LPLYRELGPENGNGYQEVVQALNGQKSSMVRVNDRGEVIVSVAVPVQRFRAVHGALML
                     STQGDDIDQMVTAERLAILKVGGVASAVMIVLSLLLASTIAGPVRRLADSAERVRRRI
                     RTRVEIPDFTRRRDEIGHLSGALRDMTDALYNRIEAIEMFAADVAHELKNPLTSLRSA
                     VETLPLARSDTSRARLLAVIEHDVKRLDRLISDISDASRLDAELQRQDMTRVDLRRLL
                     TTLTSVANEARLGNDIAVECRFEGRGLADTFSVPGHDSRLGQVISNLLVNAQSFSEAG
                     GKVRIVCRRARAEIEIIIDDDGPGIREDALERIFERFYTDRPHQGFGQNSGLGLSISK
                     QIIEAHGGRIWAENRVGPTGVDGATTIAGARFVVRLPAP"
     gene            complement(7565..8266)
                     /locus_tag="VN968_05055"
     CDS             complement(7565..8266)
                     /locus_tag="VN968_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL28641.1"
                     /translation="MPTIALVDDDRNILTSVSIALEAEGYRIMTYTDGASALDGFRTS
                     PPDLAILDIKMPRMDGMETLRRLRQKSDLPVIFLTSKDEEIDELFGLKMGADDFIRKP
                     FSQRLLVERVKAVLRRAAPKDPTAVPKETDAKALDRGLLRMDPERHTCTWKNEPVTLT
                     VTEFLILQALATRPGVVKSRNALMDAAYDDQVYVDDRTIDSHIKRLRKKFKVVDDDFE
                     MIETLYGVGYRFKEI"
     gene            8477..9211
                     /locus_tag="VN968_05060"
     CDS             8477..9211
                     /locus_tag="VN968_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HXL28642.1"
                     /translation="MQPPADFDPVKLARSLLRRSRQGALATLRPGSGGPYCSLVNVAS
                     HGDGSPILLISRLALHTQNILADSRVSLMLDERAQGDPLEGSRIMLAGRAEQAGGDAV
                     AALRRRYLNAHPSAEAFVDFKDFSFFRIRPAGAHLVAGFGRIVDLRPEQFLTETSDAG
                     ALLEAEQGAIAHMNADHRDAMNLYATRLLGAEPADWCCTGCDPGGIDMQAGAITLRLD
                     FPERVTGPGELRKMLVRLAGEARAKG"
     gene            9364..9606
                     /locus_tag="VN968_05065"
     CDS             9364..9606
                     /locus_tag="VN968_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28643.1"
                     /translation="MKPGSRLASRLVLVAWLALVLSAGFGTPSSAQKNEVAALNAGIA
                     ELSRAGKYSDAIPLAQRLLANLEQAYGPSNRDVAAC"
     gene            9697..10071
                     /locus_tag="VN968_05070"
     CDS             9697..10071
                     /locus_tag="VN968_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL28644.1"
                     /translation="MASKVLRSAPEQSGALYPRQERYADAEPLFERSLAIRERALPRD
                     HPDVWTSRNNPASLYQAEGRIPDPLPLVERTIASGHAQLRVAMSTRSVTGMLRPATPA
                     ARSSRAAGEGQAKPFWLCLQAR"
     gene            10441..12057
                     /locus_tag="VN968_05075"
     CDS             10441..12057
                     /locus_tag="VN968_05075"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HXL28645.1"
                     /translation="MQETGVRNGAFGADKFGLRNLKGVNWNFGAPQLCEHSLATGEAV
                     LSADGALCADTGVFTGRSPKDKFTVRDAVTDKNMWWAGNQSITSEQFEALYADFLGHA
                     EGMTLFAQDLYGGADPVLRIKTRVFTELAWHSLFIRTLLLRPETSELASFVPELTIID
                     LPSFRADPKRHGVRSENVVAIDFARKIVLIGGSYYAGEMKKSVFTTLNYYLPEKGVMP
                     MHCSANVGPDGDAAIFFGLSGTGKTTLSADPKRTLIGDDEHGWGGDGIFNFEGGCYAK
                     CIKLSKEAEPEIYAASKRFGAVLENVVLDAHTREPDFDDGSKTENTRSAYPLDFIPNA
                     SRTGRAGHPKNVVMLAADAFGVMPPIAKLTPAQAMYHFLSGYTAKVAGTERGLGKEPQ
                     PEFSTCFGSPFLPRDPSVYGNLLRDLIAKHDVDCWLVNTGWTGGKYGTGRRMPIKATR
                     ALLTAALNGSLRNADFRTDRYFGFAVPTSLPGVEPHILDPIKTWKDKAEFDKTARALV
                     GMFQKNFAKFEAQVDAEVRAAAPEVKLAAE"
     gene            complement(12148..13638)
                     /locus_tag="VN968_05080"
     CDS             complement(12148..13638)
                     /locus_tag="VN968_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL28646.1"
                     /translation="MNGARLLMQAGLSAEAPKETVEKIEKLVAGITDDLKVVRDRVKT
                     KNVVAALEQAETRLRDWSETGLKILKPQAGGLTVLPMPFSVAKKGDAAAAAVDDLVEM
                     VAAYGFDYRTEAEATVAADHATMLALAVATALVGLALAVAFAHSMSKPIFAAMQIAEH
                     VAAGNFTDRIELRRRDELGRLLKSLAVMQAGLKARAEEESALMSSKDQANSEQVSRRT
                     RMEGEIEAFRSTITSVLTNADAMTGELTETARTLSSIAHVAGQQSIETASSADETSAN
                     VQTVSTAAGLLGESVQAIEGQLYEATSIVRRASSMAEDANQTMGALATAAQHIDEVVG
                     FIRNIAGQTNLLALNATIEAARAGDAGRGFAVVASEVKALAIQTAKATEEISSQIAEV
                     QLATKRAVDNVGAITAVMGEIDSFTATIATAVNQQNTAAAEITKNIRQAAAGTASVAR
                     GIAGTAAASEDTNRSADLVLSSANGLSRQAAELRSSVDRFLSNVAA"
     gene            complement(13837..14157)
                     /locus_tag="VN968_05085"
     CDS             complement(13837..14157)
                     /locus_tag="VN968_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HXL28647.1"
                     /translation="MRMLASLTFVVLAGLSVGALAANLTITQKGRVFSSETVTIKKGE
                     ALTFANDDSVPHNIISTSKGNEFNLGSQPPGAATDVTFKESGEALVICAIHPRMKLTV
                     KITD"
     gene            complement(14188..15111)
                     /locus_tag="VN968_05090"
     CDS             complement(14188..15111)
                     /locus_tag="VN968_05090"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HXL28648.1"
                     /translation="MKAEYRRPALIPFPKENPYTPDKASLGKKLYFDTRISVTSAQSC
                     ASCHSPGFGWGDGLAVGVGHGMAKLGRHSPTIVNAAWSGIFMWDGRLANLEEQALGPI
                     QSPGEMNMPIEQLMERLATIAEYQPLFAAAFPGAGLSPKSLAAAIATYERTVVSEEAP
                     FDLWIEGNEQAISDEAKRGFVLFNTKAQCSSCHEGWNFTNDGFQDIGLPSADVGRGQF
                     LPQVIKMKHAFKTPGLREIARRGPYMHDGSIATLEQVVEHYDQGGVDRPSRSDLVKPL
                     GLTPQERSELVAFLKTLTSNLDPTTVPVLPR"
     gene            complement(15434..16414)
                     /gene="rocF"
                     /locus_tag="VN968_05095"
     CDS             complement(15434..16414)
                     /gene="rocF"
                     /locus_tag="VN968_05095"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HXL28649.1"
                     /translation="MSGTSKSPGSPRIALLGVPIEIGASQRGTLMGPDALRTAGIGRV
                     LEQLGLGVEDHGNLAVEATSAPEGPPPNNAKYYDDIKRWIRALSERAFLLARSGAIPI
                     FMGGDHSLSMGSVNGVARHWQELGRPVFVLWLDAHADYNTPATTVTGSMHGMSAAFLC
                     GEPGLDGLLGDRARVSISPDQLDLFGIRSIDPLERDLVRDRRIAVADMRAIDEFGVGV
                     LIRRVIERVKARNGVLHVSFDVDFLDPAVAPGVGTTVPGGATYREAHLVMELLHDSGL
                     VRSVDIVELNPFFDERGRTARVAVELLGSLFGLQITDRPTPSNAVLPDGT"
     gene            complement(16463..17281)
                     /locus_tag="VN968_05100"
     CDS             complement(16463..17281)
                     /locus_tag="VN968_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXL28650.1"
                     /translation="MLAKTQHLFDDATRVTAGDSRWQGRTSDDYWAFVGPFGGATAAT
                     ILRALIDHPQRTGDPLSLTVNFCAPIAQGAFDLDVRLIKANRSTQHWCVELSQGGAEV
                     ATLATAVFAERRPSWSHQRAEFPKSPPFEQIQPYPKVAAPWVKQYDFRFIEGDPDFSG
                     AKGDGPASAFSKLWVGDRVPRKIDMLSLMSMSDAFFGRVFHARRELVPFGTVSLTTYF
                     HADAEDLAAEDINRVLAVADARIFHKSYGDQAGELWSPQGRLLATTHQIAYFKA"
     gene            complement(17384..19930)
                     /locus_tag="VN968_05105"
     CDS             complement(17384..19930)
                     /locus_tag="VN968_05105"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HXL28651.1"
                     /translation="MSRIHDLPDQSSQGSFPVLNQPIDELALAEIKGAILAKLRLAIG
                     KDAGMATKHDWYKAAALALRDRIVHHWLTGDKQSYDAGSKRVYYLSLEFLIGRLFTDA
                     LNNMGLLPVFETALGDLGVSLSELRKCEPDAALGNGGLGRLAACFMESMATLAIPAIG
                     YGIRYDFGLFRQIIANGWQHEYPDEWLGFGNPWEFQRPEVVYHVHFGGSVEHIGEKGR
                     DRAVWHPAETVQAVAYDTPIVGWRGQHVNALRLWSARSPDPLKLDVFNTGDYLGASAE
                     EARAESICKFLYPNDESPAGRELRLRQEYFFVSASLQDLIKRHLVSDGQLRGLASKAA
                     VQLNDTHPSLAVTEMMRILCDLHNFRWDEAWKITVATLSYTNHTLLPEALETWPVELF
                     ERLLPRHLEIIYRINVAHLALADARCPGDDDFKASVSLIDEKSGRRVRMGQLAFVGSH
                     RINGVSAMHSDLMKETVFHDLNHLYPARITNKTNGITFRRWLMLANPKLADLLREACG
                     EAVLDDPGLLERLEASAGDNAFQQQFRAVKRHNKLALARLIGERLNIVVDPAALFDVQ
                     IKRIHEYKRQLLNILETVALYHAIKDEPQRDWVPRVKIFAGKAAASYRYAKLIIKLIN
                     DIAEVVNNDPEIAGRLKVAFLADYNVSLAEVIIPAADLSEQISTAGMEASGTGNMKLA
                     LNGALTIGTLDGANIEIRDHVGAENIAIFGMEALDVVVRRKQGLDATDVIRRSPHLSR
                     AIKSIESGEFSPDDPARFESIGHALRNLDHYMVSADFDAYFEAQRGIDARWQVVPAWT
                     RAAIFNVARMPWFSSDRTIREYARDIWNVPVRGPSPQGLQEPGVQREATH"
     gene            20120..20353
                     /locus_tag="VN968_05110"
     CDS             20120..20353
                     /locus_tag="VN968_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28652.1"
                     /translation="MRLMIEIIIAALLVSCITATSAAFATSGRAGRTADGLGCSFSVP
                     ANASPAARCAAIRRQCGGKFFANYCGDKLLSAM"
     gene            20753..>21047
                     /locus_tag="VN968_05115"
     CDS             20753..>21047
                     /locus_tag="VN968_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28653.1"
                     /translation="MSTIAFDQFALTRIADFARSLARLHQASRRQPIDDDAIDREFNA
                     VCLSVWGYTTDDFSDELLSEEDHAWLDSLDEAQARIFAAERGYDLVDDNCMLTD"
BASE COUNT         3784 a         6504 c         6666 g         4093 t
ORIGIN      
        1 gcgctcgata tccatcgaat cgagcacctg ttccttcatc tcgcgcgcct gcaagccgcc
       61 cgtggtctgg atcaggcggt cggccagggt cgatttgcca tggtcgatat gcgcaacaat
      121 ggcgaagttg cggatgttgg aaatagggat ggatgtcata ggcgcgggat agcattcgca
      181 ccccggggcg gcaaccataa tgcggcgttt cggcggggct tcttcacgcc aagctgatac
      241 cactgatcgc aaaaacgcgc tacgcacggg gccatgtcta ctgcatcgtt cttgcccgcg
      301 gcctcgcgcg tgcgcagccg gctatccgtc cgccgaatgg cggcgtggct tgcggcttgc
      361 gccagcgatc cgaaagccgc ccctcggctg gtaatcggtt ttgccgcggc ccacgccgtg
      421 ctgtggaccc tgatcctggt caatctcaag gcggcgcagg acgttcacat ggacgtcgcg
      481 gaagccttcg cctggggcca gaaattccag ttcggctatg gcaagcatcc gccgctggcg
      541 gggtgggtcg cgggtgtctg gttcaggatt ttcccggtcg ccaactgggc cacgtacgcg
      601 ctggcgatgg caacactcgg ctgtggcctc gcaatctgct ggctgatcgc gttacgcgcg
      661 gtcgatcgcc gccgcgcctt cttcgtcgtg gtgatgctcg cactctatcc gatcttcaat
      721 ttcaagggtt ttaagtacaa tccggatctg ctgcagctgg tgacgctgcc gctggtggtg
      781 ctggcgtttc tccatgcctt cgaaaaacgc tcgtggcgat ccggcctgtg gcttggcctc
      841 gccggtgcgc tggcgctcat gacaaagtac tgggtgctga ccatgatcgg agcgatcggg
      901 atcgccgcgc tgatccatcc cggccgctgg caattcctgc gttcgccggc gccgtgggtc
      961 gcgatcgcga cgctcgtcgt tgccatggtt ccacatctga tctggctgaa acaagtcgac
     1021 ttcgtgccgc tgacctatgc cggcgacgtc tacgggctgt cgagccgcgc gcagagcact
     1081 caactcgtcc tcggctatgt cggccacaat ctggcgctgc tcgcggcacc ggtggtgctg
     1141 gcggcacttg cgatggcatg gtcaccgcga tggtggatga cgctggcgcg gcaaccattg
     1201 gccctgttca cacgcgcatg gtcgcgcggg gagaacaccg gcgtgaatgc ctcgcaggcg
     1261 ctcaatatct ggatcatcca gatcgtggtc gcggtcggac cgccgctcgg cggcctgatt
     1321 ttcacggtct acatgaaaac cgattggggt atctcgctgt tcttcttgac gccgctggcg
     1381 ctgatcgcga tgccgtcgct gcgccttcac cggatggcgc tgtttcatat tgccgcgatc
     1441 tggctcgtga tcacgcttgc ggtgctcgcc gtgtcgccca ccatcgccgc ccgcgagctc
     1501 gccgagaatc ccaatggcgc ctcaggctat ggcgcgcgct ccgaactggc ccgcgaactg
     1561 accgaggcct ggcagacccg tttccacacg cgatgggcgg tggtcgccgg caccacggag
     1621 atcggcgagc cgatgacgtt ctacagtccg gatcatccgg cgccgttcac gcccggcgag
     1681 gtctggtcgt ccgggctgac ctcgctggag gaggcgcggc gcctcgggtt tatcggcatc
     1741 tgcgatacgt ctgatccacg gctgccggtc tgcgacgcct ggatggcggc gaatgccgat
     1801 cgcgccgagc gtctcgccat caccacgcag cgcttcttcc acggccatcc cggtccggcg
     1861 atcagctgga aggtgtatgt cgtgccgccg gcgcaatagc ctgcccgctt catatgatca
     1921 gagagactct gcggattgtc cggctactgg cgtaatgcat ggcagccagc ggatgtgaac
     1981 ctgttctcgc caagttctca ccaagacacg acaggttttc cgccgtgctt ttcgagcctg
     2041 cgagcgagtt tcggatatga tttatgatgc cgttgccgac tttcctgctc gcctccgggc
     2101 gtgccttgat gaagcggatc ctgatgcggc tctggttcga ttgaggaatg atttccgcct
     2161 gcttgccgag cttccgcggg tttcccttcc tcccccttat gccgaccaaa cccaaccgca
     2221 tccgttgatc ggtcttgcca tatcgctggt ggccggggcg ctgatcagaa ccgggcgcgc
     2281 cgccgacgcc cgcacgttca tcaatgaggc cgcggatctg ttcaatccgg agaatcgtgt
     2341 ctacgtccgc ggctcgacgt ttggcgtcct tgtcggcgcc ttcccgtttc tctttgtgcc
     2401 gtcaatcgtg gcctccatcg ctctcgggga tgggaaaagc gccgtcgaag ccgtcgtgcg
     2461 cttctgtcag agcatgcaga agagtgaact cacggcgtcc tgtcagagac gaacgcggca
     2521 ggacgattgg ttcgagtcca atccgatggt gcgggatctg ttgaaccacc acgccatcga
     2581 gccgtacggg ccgttttgtt tcgatcaccg ctggatgctc gagcgccagg aggagatcct
     2641 tcgccgcggc ccgctcgacc ggcggcttga ggcgacctac gaattcaacg aactgctcct
     2701 tgaaaacgcg cttgtcatcg agcaacccgc ccgcgggctt gcgttgatcg aagatgggtt
     2761 caattcgctg ggcccggact caagcggccc cgtcagcggt cacctcgcgt tcaacgccgt
     2821 ttgcgttctg gctgcgctcg gacgatttga tgaagcgctg gccgctgcct acgcgatggc
     2881 gcgccacggc tatggtctgc tcgcgcggtt tgatctggct gccgtcgccg aaaggatgcc
     2941 gtggtggacg gcgctgatgc gtcaaaataa ctggcttgga ccgctggctg ccacggaagc
     3001 ctaccaaagc tttctgcaga acgaggtcgc cagccggccg ttcgacgttt cggatccggc
     3061 gttcaatccg ctctgcgcgg tgcgcgacgg cgcgcttggc ggcaaggcga agcagcgttg
     3121 ctggctctcg cgggagccga tctcgccagg cgagcctgta gtccgcatcc gccgcctgtt
     3181 cgggcatgcg agtgacggcg agtttgatat tatcaaaaag gacgccttcg cggcatccgg
     3241 ctgggcaacg gcgcgacagg agtttgagac cgacaccgtt ccggtgcgac tgctgtttcc
     3301 ggatacgcgg acccacgccg agggatcgag gatctggcgg acggcggcta ttttcgcgtt
     3361 ccattacgat gttgcgcgca atccagacgc cttcgacctc gatcgtgccg tcgcgctcat
     3421 cgccgaccgt ggccctaacc cgatccggtt cgagtgggtg cgcggcaaag acgacgttgc
     3481 tcctgccttc gatcccatgg tcaatgatgt cggtcatggc gatgcggtca atttcgcctg
     3541 gaggctgcta aaggcaggct gtggccggga catatttcgc cgcgtcgcaa acctgccgca
     3601 ggaaaaagca gacaaggtct tcgccatgct cgcgacgttc gaccggaacg actgccgcgg
     3661 tgcctccgcc gaatattttg gactgcccga cctgcccaag atgatggagc gcgcatttcg
     3721 caaacggccg tcgctcgagg accacatcgc cgccgcggac ttcgccagcg cgcagccccg
     3781 ctttcgacga ggtcttacgg ccgcgatgcg ggcctatgcc ttgcatcttt attcgaacaa
     3841 ccgtccggcg gccgactggt ttctgcaggg cctcgagcat ttctcggggg cgcgctgctg
     3901 ccggttgctg actttcctca ttcatcatcc cgaggacgat tgggtacttg caacgatgat
     3961 cgagcgcgga tggctgccgc agaggttcgg gagagacgat gcctactcca acgcgctgtc
     4021 gttctattac cggacggtct tgttcaatcg catgctgcat gccccggagc agctcgagaa
     4081 ttggctggcc cccgggtggc cgggctctcg catgacgcgt tcgaaagatc gcgagaccgt
     4141 tcgactcgtc gagcaatggc ggaagaagcc gcggaggtcc cacggccgcc cgagccgcta
     4201 gaaatctctt tggcacagtc agccggctga caccccgatc ccggccaatc acgcgctttc
     4261 ttcgttgaac ttgctggcga ccagttcggt gatcgcatca acggccgctt gcgcttcggg
     4321 gccgatcgcg gagacgacga tcgatgtccc cggtcccgcg gacagcatca tcagccccat
     4381 aatggaggtg ccgccgaccg tctcgttgcc ttttgtcacc cagacttcgg cgttgaagcg
     4441 ctcgaccgtc tggacgaatt tagcggaagc gcgcgcgtgc agaccgcgct tattgatgat
     4501 gggaatttcc cgggataccg cgcccgacgg gacacttggc ccgtgatcaa tttcgggcga
     4561 cgcgtcgtcg tcggtcattt gccggcgaga acccgactgg cgatggtcac gtatttgcgg
     4621 ccggcttcct gggccatggc gatggcgtcg ggtagcgacc gctcctcgcg aaccttggca
     4681 agcttgacca gcatgggaag attgatgcct gcgagtactt cgaccttcgg ccggctcatg
     4741 caggagatcg caaggttgga aggcgtgccg ccgaacatgt cggtgagaat ggcgacgcca
     4801 tcgccgctgt cgacgcggtt gacggcttca atgatgtcac ttcgacataa atcagagtcg
     4861 tcctcggctc cgatcgtgac cgcttcgatt tgcttttgcg gacccataac atgttcgagc
     4921 gccgccttga actcgtcggc aaggcgccca tgggtcacaa gtaccagacc aatcatcaga
     4981 aactcctcgc gggtgctttt tggtgcaccg cacgaacacg cgccactttg accatccaga
     5041 gcccccgcgc aagaggggat ctacgggctt ctccccgaat ttagcgggct gaggagagcc
     5101 tgcaccggtc tattcggtcg cgatagtgtg cctcatatgg ttaccaatcc cttccaagca
     5161 attggtcgaa ggttgggtag aagttgaact cccggttgtc gtcagcgcag cgacaaccaa
     5221 tggcagtgcc gcgtaccctt cgcccacggg aatacgcggt atttttacac cgaaaatgcg
     5281 cgccagaagt gtttcaggcg gcggcaggcg ctccgcgtcg gcggcgcaga gatcgaccac
     5341 caggccgacg atagccgcgc cggtgaactc gcagcggcga ataccgagcc cgcgaatctc
     5401 gatcaggccg gccagttcgc gcgccggccg gacgacaatt tctccgtcat gtgtgtcaag
     5461 atggacacgg tcatcgccga ccaaaacggc ctcgggtatt tgcccggaac gcccggcgag
     5521 gacgagatcg aacgccagcc gcgacttgcc ggcccccgac ggtccgcgga tcagtacggc
     5581 acgatttccc accagcactg cggaagcgtg gacgctcgcc cgcgcggcat ccgtcatggc
     5641 gccggcagcc tcaccacaaa gcgcgcgccc gcgatggtcg tggcgccgtc gacgccggtc
     5701 ggaccgacgc gattctcggc ccagatgcgc ccgccatggg cctctatgat ctgcttcgag
     5761 atcgacagcc ccagcccgga attctggcca aagccctgat gcggccggtc ggtgtaaaag
     5821 cgctcgaaga tccgctccag cgcatcctcg cgaatccccg gcccgtcgtc gtcgatgatg
     5881 atctcgattt ccgctctcgc gcggcggcag acgatgcgca ccttgccgcc ggcttcggaa
     5941 aaggactggg catttaccaa cagattggaa atgacctgcc cgagccgcga atcgtggccc
     6001 ggcaccgaga aggtgtccgc cagaccccgg ccttcgaaac ggcactcgac agcaatgtcg
     6061 ttgccgagcc gggcctcgtt ggcgaccgat gtcagggtcg tgagcaggcg gcgaagatcg
     6121 acccgcgtca tatcctgccg ttgcagttcg gcatcgagcc ggcttgcatc ggaaatatcc
     6181 gagatcagcc ggtcgagccg cttgacgtca tgctcgatca ccgcaagcag ccgcgcgcgg
     6241 ctggtgtcgc tgcgcgccag cggcagcgtt tcgaccgcgg atcgcagcga tgtcagcggg
     6301 ttcttcaact cgtgggcgac gtcggcggcg aacatctcga tcgcctcgat gcggttatag
     6361 agcgcatcgg tcatgtcgcg cagcgcgccg gagagatgtc cgatctcgtc gcggcggcgg
     6421 gtgaaatcgg gaatctcgac ccgggtgcgg atgcggcggc ggacgcgctc ggcgctgtcg
     6481 gcaagccggc gcaccgggcc cgcgatggtg ctcgccagca gcaacgacag cacgatcatg
     6541 accgcggatg cgacgccgcc gaccttcagg atcgcgaggc gttcggcggt caccatctgg
     6601 tcgatgtcgt cgccctgggt cgagagcatc agcgcgccgt gcaccgcgcg gaatcgctgc
     6661 accggcacgg cgaccgaaac gatcacctcg ccacggtcgt tgacgcgcac catgctgctc
     6721 ttctggccat tcagcgcctg cacgacctcc tgatagccgt tgccgttctc cggcccgagt
     6781 tcgcggtaaa gcggcaggtc gccgcgattg agccaggtgc ggacggcgat catcgttcgc
     6841 tccgcgatgc ccggcttctc gaccgtcggt ggcggcagtt cgaaccgcag cacgtcgccc
     6901 cggccgtaga gattgcggct gtcgagaatg aggccgccgt cgcggtcgaa aatgcgggcg
     6961 cgggtcttgg tcggcgaaat cagccgccgc aacaccggcg cgacgcgctc cggattgatc
     7021 ggaaaatcca gcccggaata ttcgtccggc gcaccatagg tttcccccgg cttcagatcg
     7081 agcagccggt cgggatcgat ggtgatgctg ttggtgtcga cggttgcgga ggctgcgatc
     7141 gcaccggcga tgatctcggc cgatatctgc aggctttgcg cgcgggcgtc gatcaggccg
     7201 gcgcggaatt gcgagagata aaggatgctc gccaccagcg cgacgaggcc ggcgagattc
     7261 agcgatacga tgcggcgcgt caggctggag aagctgagcg caaagaaaaa ctgcccggcc
     7321 ctgcgcagcc agccgagcgg ccgctgccag ccctgcgcgg acgggttctc gaagttggcg
     7381 ccatccagca cgagggacga cgaggcgtcc tcggcgctca ggcttgaatc aggctgcgtt
     7441 cgatcaagca atggccaaac tgccggttgt ttcggttcta gtcttgcgat gcgcggccgc
     7501 cttcgcatcc taccccaggc aaatggggac gttcgttacc aaaacgcgcc cacgcgcccc
     7561 caaatcagat ctccttgaag cgatagccca ctccgtacag ggtttctatc atttcgaaat
     7621 cgtcgtccac gaccttgaat ttcttgcgca gccgcttgat gtggctgtcg atggtgcgat
     7681 cgtcgacata gacctggtcg tcataggccg cgtccatcag ggcgttgcgg cttttcacga
     7741 cgccggggcg cgtggccagc gcctgcagga tcagaaattc cgtgaccgtc agcgtcaccg
     7801 gttcgttctt ccaggtacag gtgtgacgtt cggggtccat gcgcaaaagc ccgcggtcga
     7861 gcgccttggc atcggtttcc ttcggcactg ccgtgggatc tttcggcgcg gcgcggcgca
     7921 gcacggcttt cacgcgctcg accagcaggc gttgcgagaa cggcttgcgg atgaaatcgt
     7981 cggcacccat tttgaggccg aacaattcgt cgatttcctc gtccttggag gtgagaaaaa
     8041 tcaccggcag atcggacttc tgccgcagcc ggcgcaaggt ctccatgccg tccatgcgcg
     8101 gcatcttgat gtcgaggatc gcaaggtccg gcggactggt gcggaagccg tcgagcgcag
     8161 acgcaccatc cgtgtaggtc atgatgcgat agccttcggc ttcgagcgca atcgagacgg
     8221 atgtgagaat gttgcggtcg tcatcgacca aggcgattgt gggcatgagc ctgctttccg
     8281 aagatgtctg aaacacgggc aattccagct atgcgccgca tgagtgggct ttgtaacggt
     8341 caacgagcaa tgcaagctgg gctgaagtgt gaccgagttc cgtaataacg accgaacgtc
     8401 catgttcccc gtcgatagag acagcagatt agcccggaaa aggcctttat tgcaatgaga
     8461 tatggagata tcattgatgc agcccccggc tgatttcgat ccggttaaat tagcccggtc
     8521 gctgctgcgg cggagccgcc agggggccct ggcgaccctg aggcccggca gcggcggtcc
     8581 ctattgctcg ctcgtgaacg ttgcaagcca tggcgacggc tcgccgattc tgctgatttc
     8641 gcgtctggcg ctgcataccc agaacatcct ggccgattct cgggtctcgc tgatgctgga
     8701 cgagcgtgcc cagggcgacc cgctggaagg atcgcgaatc atgctggcgg gtcgggcgga
     8761 gcaggccggc ggcgatgcgg tggcggcttt gcgccgccgc tatctcaacg cccacccctc
     8821 cgcggaggcc tttgtcgatt ttaaggattt ttcctttttt cggatcagac ccgccggcgc
     8881 tcacttggtg gccggtttcg ggcggatcgt ggacctcagg cctgagcagt ttctgaccga
     8941 gacgtcggat gccggggcgc tgctggaagc cgagcagggc gccatcgcgc acatgaacgc
     9001 cgatcatcgc gacgcgatga acctctatgc gaccaggctg ctcggcgccg agcctgcgga
     9061 ctggtgctgc accggctgcg atcccggtgg catcgacatg caggccggcg caatcacgct
     9121 gcggctggat ttcccggagc gggtgaccgg gccgggagag ctgaggaaaa tgctggtgcg
     9181 gctggcgggc gaagcgcgcg cgaagggata gcggataatc accacgcata ttgaacggga
     9241 gcggcagtcg ccgcaaccca cggcacgtcc gcgcgcaagt catcccgtcg catatgatct
     9301 cattcgcggg gcgcgcgcgg gcgcatcggc ggcggacgca aggcggtatg aggcgcgcgc
     9361 atcatgaaac ccggcagtcg attggcctcg cgattggttt tggtggcgtg gctggcgctg
     9421 gtcctgagcg ccggcttcgg cacgccgtcg tcagcgcaaa agaatgaggt ggccgcgctc
     9481 aacgctggga tcgccgaact aagtcgagcc ggaaaatatt ccgacgcgat acccctggcg
     9541 cagcgcctgc tcgcgaacct ggagcaggca tacggtccct ccaaccgcga cgtcgcggcg
     9601 tgttgaataa tctggcgttg ctttacggca gccaggggcg cgacgccgag gccgagccct
     9661 tgtacaggcg agcgcacgcg atcatggaaa agatgtttgg cctcgaaagt gctgaggtcg
     9721 gcgcctgagc aatctggcgc cctttacccg cggcaggagc gctacgccga tgcggagccg
     9781 ctgttcgagc ggtcgctggc gatccgcgaa agggcgcttc cgcgcgacca tccggatgtc
     9841 tggacctcgc ggaacaatcc ggcttccctc taccaggccg agggccgcat ccccgatccc
     9901 ttgccgctgg tggaaaggac gatcgccagc ggacacgcgc agctgcgcgt tgccatgtcg
     9961 acaagatcgg taacgggaat gttgcgtccg gcaacgccgg ctgctcggtc cagtcgagca
    10021 gctggtgaag gacaagcgaa gcctttctgg ttgtgccttc aggcgcgctg acagcgctgc
    10081 cattccatcc agtggtctgg ggtccgtttg cgctgatcgg cgagggcgcg gcccgatagg
    10141 tcgggggtgt gcaccgcagc attggtatta cggaggtcaa tttacaaaat ttgcgactct
    10201 tggtcggttt tggacggcga cggcttctca cgcaacgcgc cgatcaggtc cgcgctgtgt
    10261 gagctggatc aatgacccgt tgagaataaa ccaaatacca agggatcggc ttggcaactc
    10321 cggcgttcat cagtattagg gttccggacc gaagccggaa ggctattgac ggtcatcgcg
    10381 agaggcgcgt ctggcggcgt cggcgcgtga gttacgcgcg ggttctggga ggattattcg
    10441 gtgcaagaga cgggcgtacg caacggtgcc ttcggtgccg acaaatttgg tttaagaaat
    10501 ctcaaaggcg tgaactggaa ttttggcgcg ccgcaacttt gcgaacactc cctggctacc
    10561 ggtgaagccg tgctttccgc cgatggcgcc ctgtgcgcgg acaccggcgt gttcacgggc
    10621 cgcagcccca aggacaaatt caccgttcgc gacgccgtga ccgacaagaa catgtggtgg
    10681 gccggcaacc agtcgatcac gtcggaacaa ttcgaagcgc tctacgccga cttcctcggg
    10741 catgccgaag gcatgacgct gttcgcacag gatctctatg gcggcgccga tccggtcttg
    10801 cggatcaaga cccgcgtctt taccgaactc gcctggcatt cgctgttcat ccgcacgctg
    10861 ttgcttcgcc cggagacatc cgagctcgcg agcttcgtgc cggaactgac catcatcgat
    10921 ctgccgagtt tccgcgccga tcccaaacgc cacggcgtgc gttcggagaa cgtggttgcg
    10981 atcgatttcg cccgcaagat cgtcctgatc ggcgggtctt attatgccgg cgagatgaag
    11041 aagtcggtat tcaccacgtt gaattattac ctgcccgaaa agggcgtgat gccgatgcac
    11101 tgctcggcca atgtcggccc cgacggcgat gctgcgatct tcttcggtct gtccggcacc
    11161 ggcaaaacca cgctctcggc cgaccccaag cgcacgctga tcggcgacga cgagcacggc
    11221 tggggcggcg acggcatctt caattttgaa ggcggctgct acgccaagtg catcaagctg
    11281 tcgaaagaag ccgaacccga aatctatgcc gccagcaagc gttttggcgc ggtgctggaa
    11341 aacgtggtgc tcgacgcgca cacccgtgaa ccggatttcg acgatggctc gaaaaccgaa
    11401 aatacccgtt cggcctatcc gctcgacttc atccccaacg cctcgcgcac cggccgcgcc
    11461 ggtcatccga agaatgtggt gatgctggcg gcggatgcgt ttggcgtgat gccgccgatc
    11521 gcgaaattga cgccggcgca ggcgatgtat cacttcctgt ccggctatac cgcgaaggtg
    11581 gcgggcacgg agcgcgggct cggcaaggag ccgcagccgg agttctcgac ctgcttcggc
    11641 tcgccgttcc tgccgcgcga tccttcggtg tacggcaatc tgctgcgcga cttgattgca
    11701 aagcacgatg tcgattgctg gctggtcaac accggctgga ccggtggcaa atacggtacc
    11761 ggccgccgca tgccgatcaa ggccacgcgg gcgctgttga ccgcggcgct gaacggctcg
    11821 ctgcgcaacg ccgatttccg caccgaccgc tattttggct tcgcggtgcc gacctcgctg
    11881 ccgggcgtcg agccgcatat cctcgatccg atcaagacct ggaaggacaa ggccgagttc
    11941 gacaagaccg cgcgcgcctt ggtcggcatg ttccagaaga atttcgcgaa attcgaagcc
    12001 caggtcgacg ccgaggttcg cgccgcggca cctgaagtga aactcgcggc ggagtgaccc
    12061 gcagggtcat tccggttcgc gctagcgcga acccggaatc tcgacctaag taagggcggt
    12121 cgcggggccg cactttttac caagttacta tgccgccacg ttggacagga agcgatcaac
    12181 cgacgagcgc agttccgcag cctgacggga caggccgttt gcagagctga gcaccaggtc
    12241 tgccgagcgg ttggtatcct cgcttgccgc ggcggttcct gctatgcccc gtgcgacgct
    12301 tgccgttccg gcggcagcct gacggatatt tttggttatt tcagccgccg cggtattctg
    12361 ctggttcacc gcggtagcga tcgtcgcagt gaagctgtcg atctctccca tgacggccgt
    12421 gatggctccg acattgtcca ccgcccgctt tgtggccagc tgcacttcgg cgatttgaga
    12481 cgagatttcc tcggtggcct ttgcggtctg gatcgccagc gccttcactt ccgaagcaac
    12541 cactgcgaag ccgcggccgg catcgccagc ccgcgccgcc tcgatggtgg cgttgagggc
    12601 cagcaggttg gtctggccgg cgatgtttcg gatgaaacct accacctcat caatatgctg
    12661 ggcagcggtc gcgagcgctc ccattgtttg gttggcgtcc tcagccatgc tcgaggcgcg
    12721 ccgtacgatg ctggtggctt cgtagagttg tccctcgatg gcctgcacgg attcgccaag
    12781 caggcccgcg gcggtcgaga cggtctggac gttggcggac gtctcatcgg cgctcgatgc
    12841 cgtctcaatc gattgctgac cggccacgtg ggcaattgac gacagcgttc gcgcggtctc
    12901 ggtcaactcg cctgtcatgg cgtccgcgtt ggtaagcacg gaggtgatgg tggaacggaa
    12961 tgcttcgatt tccccctcca tgcgtgttcg ccggctcacc tgctccgagt ttgcctggtc
    13021 cttcgacgac atcagggcgg attcctcctc ggcgcgggcc ttcaggccgg cctgcatcac
    13081 ggcaagggat ttcagaagac gaccaagctc atcgcgccgc cgcagctcga tccggtcggt
    13141 gaagttgccg gccgccacat gttccgcaat ctgcatggcg gcgaagatcg gtttgctcat
    13201 ggagtgcgcg aatgcgaccg cgagcgctaa cccgacgaga gccgttgcaa ccgcaagcgc
    13261 gagcatggtg gcgtggtctg ccgcgaccgt cgcctcggct tccgtgcggt agtcgaagcc
    13321 ataagcagcg accatttcca ccagatcgtc cactgccgca gcggcagcat cgcccttctt
    13381 ggcgaccgag aaaggcatgg gaagcacggt caggccgccg gcctgcggtt tgaggatctt
    13441 cagcccggtt tccgaccagt cacgaagcct ggtttcagcc tgctccagag cggcgacgac
    13501 attcttggtt ttgacccgat cccgaaccac tttcaggtcg tccgtgatcc cggcgaccag
    13561 tttttcgatc ttctcgaccg tctccttcgg cgcttccgcg ctgaggccag cctgcatcag
    13621 aaggcgtgcg ccgttcaacg cggcatgcgc cgaacgcgca tgattgattc ccatcagcgg
    13681 tccatcgtag agccgcacca caagatcgcc ggaggtggag atgccacgca taccgtaaaa
    13741 cgccagcccg caggcaaggg cgatgacgac actgaaggcg caaaaaaact tgtaacgaat
    13801 cgacatagaa attgctccgg cgaacttcca aatggactag tccgtaatct tcaccgtcag
    13861 cttcatccgc ggatgaatcg cgcagatgac cagtgcctcg ccggattcct tgaaggtcac
    13921 atccgtcgcc gcgccgggcg gctgcgatcc gaggttgaac tcgttcccct tgctcgtcga
    13981 aatgatgtta tggggcacgc tgtcatcgtt tgcgaaagtc agcgcctctc ccttcttgat
    14041 ggtgacggtt tcggacgaga agacgcggcc cttctgggtt atcgtcaggt tggcggccag
    14101 cgcgccaacc gacaggccag cgagaacgac aaaagtgaga gaagctagca tgcgcatagg
    14161 atgttcctgc ggttggcgtt gactggttca gcgggggagg accggcacgg ttgtcggatc
    14221 gagattgctg gtcaaggttt tcaggaaggc gaccaattcg gaccgctcct gcggcgtgag
    14281 accgagcggt ttcacgaggt cggagcggct cggccggtcg acgccgccct ggtcataatg
    14341 ttcgaccact tgttcgagcg tggcgatcga cccatcgtgc atgtaaggac ctcgcctggc
    14401 gatttcgcgc agccccggcg tcttgaaagc gtgcttcatc ttgatgacct ggggcaggaa
    14461 ctgcccccgg ccgacatccg cgctaggaag gccgatgtcc tggaaaccat cattggtgaa
    14521 gttccagccc tcatggcacg acgagcattg agccttggtg ttgaagagga cgaagccgcg
    14581 ctttgcctcg tcggaaatcg cttgctcgtt tccctctatc cagaggtcga acggcgcctc
    14641 ctcggaaacc acggttcgtt cgtaggtcgc aatggccgct gcgagcgact tgggagatag
    14701 accggcgccg ggaaaggccg ccgcgaacag cggctggtat tcggcgatgg tagccagccg
    14761 ctccatcagt tgctcgattg gcatattcat ctcgcctgga gactggatcg gaccgagcgc
    14821 ctgttcctca aggttggcaa gccgaccgtc ccacatgaag attccactcc atgcggcatt
    14881 gacgatggtc ggcgagtgcc ggccgagctt cgccatgccg tgaccgaccc cgaccgccag
    14941 tccgtcgccc caaccaaagc ccgggctatg acagctcgcg caggactgcg ccgaggtgac
    15001 cgagatacgg gtatcgaaat agagtttctt gccgagcgac gccttgtcgg gcgtgtaagg
    15061 gttttccttt gggaacggga tcagcgccgg acgccggtat tctgccttca tcgcgtcaat
    15121 ggctacagcg gctttcgggc tgggttcggt gcgacgcatc tctccgacaa tcgctgcacc
    15181 aaagctaagg tttgtgaatc cgatcaagcc gagggcgacc gccgccacga ccggaccgga
    15241 ccgggcggaa cgaatgaatt cggatgcagg catgttaggt ccttgaagcg ggcttgggag
    15301 atgccacgtc caagaaataa agattgtttg ataacggccc cataattttg gcactggtaa
    15361 aactacgagc cagcggaagc cggcgcttgt ttcggattcg cgatcggctg tcatccgaat
    15421 tgccgatcag ctttcaggta ccgtcgggaa gcaccgcgtt gcttggcgtc ggacgatcgg
    15481 tgatctgcaa accgaacagg ctgccgagca gttccaccgc gacacgcgcg gtgcggccac
    15541 gttcgtcgaa aaagggattg agctcgacga tgtcgacgga gcgcaccaat cctgaatcgt
    15601 gcaagagctc catgacgaga tgcgcctcgc gataggtcgc gccgcccggc acggtggtgc
    15661 cgacgcccgg cgcgaccgcc gggtcgagaa aatcgacgtc gaagctgaca tgcagaacgc
    15721 cattgcgcgc cttgaccctt tcgatgaccc tccggatcag gacgccgacg ccgaactcat
    15781 cgatcgcacg catgtccgcc actgcgatgc ggcgatcgcg gacgagatcc ctttcaagcg
    15841 gatcgatcga gcggattccg aacagatcga gttgatcggg actgatcgag acccgcgccc
    15901 tgtcacccag caggccatcg agcccgggtt cgccgcagag gaacgccgcc gacatgccat
    15961 gcatgctgcc ggtcacggtc gtcgccggcg tattgtagtc ggcgtgggcg tcgagccaca
    16021 gcacgaaaac cggacggccc aattcctgcc agtgtcgcgc aacgccgttt accgatccca
    16081 tcgacaggct gtgatcgccc cccatgaaga tcggaatggc gcccgagcgc gcgagcagaa
    16141 aggcgcgttc gctgagcgcg cggatccaac gcttgatgtc atcgtaatat ttcgcattgt
    16201 ttggcggcgg accttcgggg gccgatgtgg cctctaccgc aagattgccg tgatcctcga
    16261 cgccaagtcc caattgctcg agcacgcggc cgatgcccgc cgtccgtaag gcgtctggtc
    16321 ccatcagggt gccgcgttgc gaagccccga tctcgatggg aaccccgagc aaggcgatcc
    16381 gcggcgagcc ggggcttttc gatgtgccgg acatggtcgg tgtgactttc aaggctggaa
    16441 cggaagcaac gcctgaatac ggctaggcct tgaagtacgc tatctgatgc gtggtggcga
    16501 gcaggcggcc ctgcggtgac cagagttccc ccgcctgatc cccataactt ttgtggaaga
    16561 tcctggcgtc ggcgacggcg agaacgcggt tgatatcttc cgccgcaagg tcctcggcgt
    16621 cggcgtggaa ataggtcgtc agcgataccg tgccgaacgg caccagttcg cgcctggcat
    16681 gaaagacccg gccgaagaac gcatccgaca tcgacatcag cgacagcatg tcgatctttc
    16741 gcggaacccg gtcgccgacc catagcttgg agaaggcgct cgccggccca tcccctttcg
    16801 cgccggagaa atccggatcg ccctcgatga aacgaaaatc atattgcttg acccacggcg
    16861 ccgccacctt cggatagggc tggatctgct cgaacggcgg acttttggga aattcggccc
    16921 tttggtgcga ccatgacggg cggcgttcgg caaagacggc ggtcgcaagc gtcgcaacct
    16981 cggcgccgcc ttggctgagc tcgacacacc aatgctgcgt cgaccggttg gccttgatca
    17041 ggcggacgtc gagatcgaaa gcgccttgcg cgatcggcgc gcaaaagttg acggtcaacg
    17101 acagcggatc gccagtccgc tgcggatgat cgatcagcgc ccgcaggatc gtggcggcgg
    17161 tcgcgccgcc gaacgggccg acaaacgccc aataatcatc gctggtccgc ccctgccagc
    17221 ggctatcgcc ggcggtcacc cgggtggcgt cgtcgaacag gtgctgggtc ttcgcaagca
    17281 tgagagtcct tgcgcaattg gcggagcgcc ttccagggca gtatcacggc cgcctggtgc
    17341 gaattaaatg acgcgggagg taaatgttgt tcgatgatcg gcgtcagtgg gttgcctcgc
    17401 gctgcacgcc cggctcctgc agcccctgcg gcgaaggccc gcgcaccggc acgttccaga
    17461 tatccctggc atattcgcgg atggtgcgat cggacgagaa ccatggcatc cgcgcgacgt
    17521 tgaagatagc ggcccgcgtc catgccggca ccacctgcca gcgggcgtcg atgccgcgct
    17581 gggcctcgaa ataggcgtcg aaatccgcgc tgaccatgta gtggtcgaga ttgcgcagcg
    17641 cgtgcccgat cgattcgaac cgggccggat cgtcgggcga gaattcgccg ctctcgatcg
    17701 atttgatggc gcgcgaaaga tgcggcgacc gcctgatcac gtcggtggcg tccagcccct
    17761 gcttgcgccg caccaccaca tcgagcgcct ccatgccgaa gatcgcgata ttctcggcgc
    17821 cgacgtgatc gcggatttcg atattggcgc cgtcgagcgt gccgatggtc agcgcgccgt
    17881 tcagtgcgag cttcatgttg ccggtgccgg aggcttccat gcccgcggtc gaaatttgtt
    17941 ccgagagatc ggcggcggga atgatcacct cggcgaggct gacattgtag tcggccagaa
    18001 acgcgacctt caggcggccg gcgatctcag gatcgttgtt gacgacttcg gcgatgtcgt
    18061 tgatcagctt gatgatcagc ttggcatagc gatagctcgc cgccgccttg ccggcaaaga
    18121 tcttcacgcg cggcacccag tcgcgctgcg gctcgtcctt gatcgcgtga tacagggcta
    18181 cggtttcaag gatgttgagc agttgccgct tgtactcgtg gatgcgcttg atctggacgt
    18241 cgaacagcgc ggcgggatcg accacgatgt tgaggcgttc gccgatcagc cgggccagtg
    18301 caagcttgtt gtggcgcttg accgcgcgga attgctgctg gaacgcgttg tcgccggcgc
    18361 tggcttcgag acgttcaagc aggccgggat cgtcgagcac ggcttcgccg caggcctcgc
    18421 gcaacagatc ggccagcttc gggttggcca gcatcagcca gcggcggaaa gtaatgccgt
    18481 tggtcttgtt ggtgatgcgg gcgggataaa gatgattgag atcgtggaac acggtctcct
    18541 tcatcagatc ggaatgcatc gccgagacgc cgttgatacg gtgcgatccg acgaacgcca
    18601 attgtcccat ccgtaccctg cggccggatt tttcgtcgat cagggatacc gaggccttga
    18661 agtcgtcatc gccgggacag cgcgcatcgg caagcgcaag atgcgcgaca ttgatgcgat
    18721 agatgatctc cagatgccgc ggcaacaggc gttcgaacag ttcgaccggc caggtctcga
    18781 gcgcttccgg cagcagggtg tggttggtgt aggacagcgt cgccaccgtg atcttccacg
    18841 cctcgtccca gcggaagttg tggagatcgc agagaatgcg catcatctcg gtgacggcga
    18901 gactgggatg ggtgtcgttg agctgcacgg ccgcctttga cgccagcccg cgcaactggc
    18961 cgtcggatac gagatggcgc ttgatcagat cctgcagcga ggccgagacg aagaaatatt
    19021 cctgacgcag ccgcagctcg cggcccgccg ggctctcgtc attcgggtag aggaacttgc
    19081 agatcgattc ggcgcgcgct tcttccgcgc tggcgccgag atagtcgccg gtattgaaca
    19141 cgtcgagctt gagcgggtcg ggcgatcgcg ccgaccacag ccgcagcgca ttgacgtgct
    19201 ggccgcgcca gcccacgatc ggggtgtcgt aggcgacagc ctgcacggtt tccgccggat
    19261 gccagaccgc gcggtcgcgg cctttctctc caatgtgttc gacgctgccg ccgaaatgca
    19321 cgtggtagac cacctcgggc cgctggaatt cccaggggtt gccgaagccc agccactcgt
    19381 cgggatattc gtgctgccag ccattcgcga tgatctggcg gaacaggccg aaatcgtagc
    19441 ggatgccata gccgatggcg ggaatggcca gtgtcgccat gctctccatg aagcacgccg
    19501 ccagccgtcc aagaccgccg ttgccaaggg ccgcgtccgg ttcgcacttg cgcaactccg
    19561 agagactgac cccgagatcg cccagcgcgg tctcgaacac cggcaatagc cccatattgt
    19621 tcagtgcatc ggtgaacagg cggcctatca ggaattccag actgaggtaa tagacccgct
    19681 tgctgccggc gtcgtagctc tgcttatccc cggtcagcca atggtgcacg atgcggtcgc
    19741 gcagcgccag tgcggcggcc ttgtaccagt cgtgcttggt ggccatgccg gcatccttgc
    19801 cgatcgcaag cctcagcttc gcaagaatcg cgcctttgat ttcggccagc gccagctcgt
    19861 cgatcggctg gttgagcacc ggaaaactcc cctgggaaga ctgatctggc aaatcgtgga
    19921 tcctggacat ttgagtctca gccaaacaat acgcgtgttg ggccgggatc gaaacccgct
    19981 ttgaggtttc gtttgtgcgg tcctcgcatg gtttatttat gcaaggactg ggccgatttg
    20041 ccgcaatctc ccatgcgccg atgtggtggt agggtaaaat ccgccgccgt cgggataatt
    20101 ttgagggaag gaccgggcca tgagattgat gatcgagatt atcatcgcag cactgctggt
    20161 gtcctgcatc accgccacca gcgcggcctt cgccactagc ggcagagcgg gccgaactgc
    20221 cgacggcctg ggctgctcat tcagcgtccc ggcaaatgcc tcgccggccg cgcgctgcgc
    20281 ggccatccga aggcaatgcg gcggaaaatt tttcgccaat tattgcggcg acaagctctt
    20341 gtcggcgatg tgaaacaaag ccacggacag agacgatttc caatcgcgcc atggcaatgc
    20401 ttgttcacga gatttgggaa gacgtcggcg atgacggcag cgtattgccg ggactttgtc
    20461 tcgctggtcc ggatggcgag gcgtttcgca aactctacac agggatgccc ggtgcaccgt
    20521 taggttcgag gccggcagct atttcgaggc aatgacgatc tattatcggc atcatggttg
    20581 gggcatacac aaccgacttc gcttctgatc gcgagcccta tcctgaagat cgggcacaac
    20641 ggcaatcgtt cggaacaaat taacaacact ttagcaagtc tttgatatat catagtgatt
    20701 cggcgcagcg ccgatacaac atctagggtc tatccgaaat cttgaggact acatgtccac
    20761 catcgccttc gatcaattcg cgctcacccg catcgccgac ttcgcccgct cgctggcgcg
    20821 gctgcatcag gcctcccgtc gccagccgat cgatgacgac gcgatcgacc gggagttcaa
    20881 cgccgtctgc ctgtcggtgt ggggctacac cacggacgat ttcagcgacg agctgctttc
    20941 cgaagaggat cacgcgtggc tcgattcgct cgacgaggcg caggcgcgca tcttcgccgc
    21001 cgagcggggc tatgacctcg tcgacgacaa ctgcatgctg acggatt
//
LOCUS       DATNOI010000135         7292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2437785,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000135 DATNOI010000000
VERSION     DATNOI010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7292
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2437785"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VN968_05120"
     CDS             complement(<1..222)
                     /locus_tag="VN968_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28654.1"
                     /translation="MSPNADGNKPIDPVGSFARTTAQGLGSLWGMMLEHLSEKHAKAA
                     ASWSENQEGGVGRTPDGILVTSKELVMQVL"
     gene            complement(240..548)
                     /locus_tag="VN968_05125"
     CDS             complement(240..548)
                     /locus_tag="VN968_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28655.1"
                     /translation="MPKPIDGRIDASRQLKGDQYQTIALISSLRHEFLSFSGKIPCCF
                     RPSMMAVAPSGNEFSAGSDFADHHLGNAASFMKTKESVHRLLDGLINRMHHGAGCTAR
                     "
     gene            complement(659..4000)
                     /locus_tag="VN968_05130"
     CDS             complement(659..4000)
                     /locus_tag="VN968_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL28656.1"
                     /translation="MDVGTWLSGLGLSQYEAVFRESAIDADVLTELTDQHLKDLGVAL
                     GHRLKMLRAIRELAGEAPVKASAAKPQDAADRRQLTVMFCDLVDSTALTAQLDPEDMG
                     DLLRAFQRAVAVAVTRFDGHVAKWTGDGASVYFGYPRAHEDDAERATRASIALVEAVG
                     KLRRERGIALEVRIGISTGLVVVGELIGEGEARERGVVGDTPNLAARLQTLAEPGTVV
                     VSEATRRLLGRHFVLEPLGPLELKGFKSPVPAWQILRVSENVSRFEASRSEAMTPFVG
                     REQEIALLINRWRRATKGEGQVLLLSGEAGIGKSRILAALRERIGDERHLALRYQCSP
                     HHGNDAFHPVIGQIWRGAGLVSGEPAAARLDKLETMILSAGLDIGEFVPYLASLLAIP
                     TEGRYPALEMSPSEVKERTIAVLIAMVVGIAKSVPVVMLLEDAHWIDPTSLDLTSRLV
                     EKVQDLAVLIVMTARPEFVAPWIGRPHFTAVALNRFERSQVVTMIDRIMSSRALPDEV
                     LDQIIAKTDGVPLFVEELTKSVLESGLLREENGAYVLAAALTPLAIPSTLHDSLTARL
                     DRLSPIKEIAQIGATIGREFSYDLLEAVSPIRGQALQNALRQLMETELIYRRGTPPKA
                     SYIFKHALVQDTAYVSLLRGRRQRIHADIAQVLKERSAKEEYQPAIIAHHFTEAGLAE
                     QAAPYWLAAAELALSQSAPVEAEHHASAGLALIPRVTDGPERDALELGLLVARANAMI
                     PLKSISAPETFAALADAKQLLDRGVGTDLQRVSILYGLCSATTLTARLQQALDFAHQI
                     IEVAERQDDPTYRVVGYRQLGTLQFYAGKNREALQSLQKGSQYCDPRRQRALGYRFSW
                     DQGVALLSFEVLVRLSLGLLDSAAQLSERVRIELLSHGHATTVASATFSSQTWPKLVL
                     GDLEALERESAELAAYCAEKRVEQIRLLASYHYAYACAMRDPVERNIAVLRAALDALR
                     RSGGNAGNSIIKCNLAEALLTAGDLTGAEAVLEEAFAFVEQSGEQYWLAGLHRLCGHV
                     ALKRPEPDRLHAEACFKKAIEVARSQEARLLELRAAVDLAQLWRDTGSDRDPRAMLEP
                     ILAAIEGGETTRDVRNARAALAGLV"
     gene            complement(4328..4735)
                     /locus_tag="VN968_05135"
     CDS             complement(4328..4735)
                     /locus_tag="VN968_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28657.1"
                     /translation="MLRQIVHSALMVGVVIALSGCGIADSRSPVPEFMRAAASEAPPP
                     EPPPDVGRLIHEQLDSVFTATSYPRQVRVSPPHHEPYGSGWSACVRAELTSVTGKQLG
                     TETYRVTISGGTIVDRRRAEADDNCVSEIYQPI"
     gene            4917..5150
                     /locus_tag="VN968_05140"
     CDS             4917..5150
                     /locus_tag="VN968_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28658.1"
                     /translation="MKTLTVATVMLALIAMPAHAQMQGKRHTGSDERKTDQKENKVDE
                     KAYKAALERIPDSKEKYDPWGIARPADTTKKPK"
     gene            5226..6746
                     /locus_tag="VN968_05145"
     CDS             5226..6746
                     /locus_tag="VN968_05145"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXL28659.1"
                     /translation="MLRSVLRHALLAAGILLGSHAAFAENRVALVIGQSNYHSVIALP
                     NPANDARAMSRLLGDAGFDVTSAADLSQNEMREKVSDFAAKVAAKGPDTVALVFYAGH
                     GLQIDGENFLVPVDVDPKREADIPLQAIRLNDVLNTLTSVPSKTRILLLDACRNNPFP
                     AINGTAGRGLAIVDAKTGAPGTFMSFSTSPGAEAEDGSGADSPYTTALLSAAKEPGLS
                     IEDTFKRVRVSVNKVTEGRQTPWDSSSLTDDFRFFSGPSAAAPAPKPVAVKRTVDQWR
                     RELQGQPVEAANELIVADGTEEAYEAFVGLFAQPPFGPQAREWLDRHRRMVAWNNAVI
                     INTAAAYRAFLVQYPDSDLTVTARKLEDRLRYRPNIAGSLALAVGGAPNGSGGSAPAS
                     PGGAPLPTNASLAAPTCPCGGTPALPLRKVDLPPKKHADPEPPKRASVRPPPRGPREP
                     PPDDDVVVYRRSPPPDYYEPSPVPSIGIGIGMGMGRIGGGGGDYRGGGMVGRRGGY"
     gene            complement(6774..7202)
                     /locus_tag="VN968_05150"
                     /pseudo
     CDS             complement(6774..7202)
                     /locus_tag="VN968_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
BASE COUNT         1344 a         2298 c         2298 g         1352 t
ORIGIN      
        1 aagaacctgc atcaccagtt ccttgctggt caccagaatg ccgtcggggg ttcggccgac
       61 gccgccttcc tggttttccg accagctcgc tgcggccttg gcatgcttct cggaaagatg
      121 ttcgagcatc atgccccaca aactgccgag gccctgggcg gtagtccttg caaagctgcc
      181 cacggggtca atcggtttgt ttccatcagc atttggcgac attggtttct cctgtaaaat
      241 tatcgcgcgg tgcagcccgc gccgtgatgc atgcgattga ttaacccgtc caatagtcga
      301 tgaacgcttt ccttcgtctt catgaagctg gcggcattgc cgaggtgatg gtccgcaaag
      361 tcagagcccg cactgaattc gttccccgac ggcgcgaccg ccatcatcga aggcctaaaa
      421 cagcaaggaa ttttcccgga aaatgataga aattcgtgac ggagagaaga aataagagca
      481 atcgtttggt attgatcccc ttttagctgg cgcgatgcgt cgatccggcc atcaatgggc
      541 tttggcattt tgcaagccga ggttgccttt tgcaatgcaa attgcggccg gtcgataaat
      601 ctttcgttga tccggcagaa ggcattttgt catcggcgtg atcgagatcg ttccgcatct
      661 aaaccagccc ggccagcgcg gcgcgggcgt tgcggacgtc gcgcgtcgtc tcgccaccct
      721 cgatcgcggc caggatcggc tccagcatcg cgcggggatc gcggtccgac ccggtgtcgc
      781 gccacaactg cgcgaggtcg acagccgcgc gcagttcgag cagacgcgct tcctgggagc
      841 gggcgacctc aatggctttt ttgaaacagg cttccgcgtg cagccgatcc ggctccggcc
      901 gtttgagcgc aacgtgcccg caaagccgat gcaaaccggc gagccagtat tgctccccgg
      961 attgctcgac gaaggcgaaa gcttcctcca gaacggcctc ggctccggtc aaatctcccg
     1021 cggtcagcag cgcctcggcg agattgcatt tgataatgga gtttccggca tttccacccg
     1081 atctgcgcaa cgcgtcgagc gcggcacgaa ggaccgcgat gtttctctcg acagggtcac
     1141 gcatggcgca ggcgtaggcg tagtggtagc tggcgagcag acgaatttgc tcaacccttt
     1201 tttcggcgca gtaggccgcg agttcggcgc tctcacgctc cagggcctca agatcaccca
     1261 acacgagctt gggccaggtc tgggacgaaa atgtcgcaga cgcaacggtg gtggcgtgcc
     1321 cgtgacttag cagttcaatc cgcacccgtt cgctgagttg cgcggcgctg tcgagcaggc
     1381 cgagcgacag acgcaccaat acctcgaagg aaaggagagc gacgccttga tcccaactga
     1441 accgatagcc aagcgcccgc tgccggcgtg ggtcgcagta ttggctaccc ttttgcaggc
     1501 tctgtaacgc ctcacggttc tttccggcat aaaactgaag cgtcccgagc tgacgatatc
     1561 cgacgacccg gtaggtcgga tcatcctggc gctcggcgac ttcgatgatt tgatgggcaa
     1621 aatcgagcgc ctgctgcaat cgcgccgtca gggtggtggc ggagcaaagc ccgtacagaa
     1681 tggacacgcg ctgcaaatcc gtgccgacgc cccggtccag cagctgtttc gcatccgcca
     1741 gggcggcgaa tgtttccggc gcagaaatgc ttttcagagg tatcatcgcg ttggcgcggg
     1801 cgaccagaag gcccagttcg agcgcgtcgc gttccggtcc gtcggtcacg cgcgggatca
     1861 acgccaggcc ggcgctggcg tgatgctccg cttccaccgg ggccgactgc gagagcgcga
     1921 gttcagcggc cgccagccag tacggcgccg cctgctcggc aagccccgct tcggtgaaat
     1981 gatgtgcgat gatggcgggc tggtattcct ccttggcgga gcgctccttc agtacctgcg
     2041 cgatatcggc atgaatgcgc tggcgccggc cgcgcaacag cgacacgtag gcggtatcct
     2101 gcaccagcgc atgcttgaaa atgtagctgg ccttgggcgg cgttccgcgg cggtaaatca
     2161 gttcggtttc catgagctgc cgcaatgcgt tctgcaatgc ctgccctctg atcggcgata
     2221 ccgcttccag gagatcgtag gagaactcgc gcccgatcgt cgcaccgatc tgcgctatct
     2281 ccttgatcgg cgacaggcga tcgagccggg ccgtcagcga atcgtgcagc gtcgagggga
     2341 tggccagcgg cgtcaacgcg gcggcgagga cataggcgcc gttctcctcg cgcaacaggc
     2401 cggattccag cacgctcttg gtcagctcct ccacgaatag cggcacgccg tcggtctttg
     2461 caatgatctg atcgagcact tcgtcaggca gcgccctgct tgacatgatg cgatcgatca
     2521 tggtgacgac ctggcttcgt tcgaaacggt tgagcgcaac ggcggtgaaa tgcggacgac
     2581 cgatccaggg cgccacgaac tccggccgag ccgtcatcac aatcaacacg gcgaggtctt
     2641 gcactttctc gacgagccgg ctggtcagat cgagcgaggt gggatcgatc cagtgcgcat
     2701 cttcgagcag catcacgacc ggcaccgatt tggcaattcc gactaccatc gcgatcagca
     2761 cggcgatggt tcgctccttg acctcgctcg gggacatttc gagtgccgga tatcgcccct
     2821 cggtcgggat cgccagcagc gacgcgagat aaggcacgaa ctcgccgatg tccaagcccg
     2881 cgctcaagat catcgtctcc aatttgtcga gccgcgcggc ggccggttcg ccgctgacaa
     2941 gacccgcgcc acgccagatt tggcctatca ccggatggaa cgcgtcgttg ccgtgatgcg
     3001 gcgagcattg atagcgcaag gcaagatggc gttcgtcgcc gatgcgttcg cgcagcgccg
     3061 ccagaatgcg cgacttgccg atgccggcct ctcccgacaa cagcaggacc tgaccctcgc
     3121 ccttggtagc acggcgccag cggttgatga gaagcgcgat ctcctgctcg cggccgacga
     3181 acggtgtcat ggcctccgag cgcgacgcct cgaaccggct gacgttctcg cttacgcgca
     3241 ggatctgcca ggcgggaacc ggggacttga aaccctttaa ttccagcggc ccgagcggct
     3301 ccaatacgaa gtgcctgcca agcaatcttc gcgtcgcttc ggagaccacg acagtaccgg
     3361 gctctgccag ggtttgcagt cgcgcggcga gattgggtgt gtctccgacg acgccgcgct
     3421 cgcgcgcctc gccttcgccg atcagttcgc cgaccacgac caggccggtt gaaataccga
     3481 tccgcacctc gagtgcgatg ccgcgctcgc gccgcagttt ccccaccgct tccaccagcg
     3541 cgatgctggc gcgggtggca cgttcggcgt cgtcttcatg ggcgcgggga tagccgaaat
     3601 agacgctcgc gccgtccccg gtccacttgg cgacgtggcc gtcgaagcgc gttaccgcca
     3661 ccgcgaccgc gcgctgaaac gcgcggagca ggtcgcccat gtcctccgga tcgagttgcg
     3721 ccgtcaacgc tgtcgagtcg acgagatcgc agaacatcac ggtcaattga cgtcgatcgg
     3781 cggcgtcttg cggcttcgcc gcagatgcct tgaccggtgc ttcaccggca agctcgcgga
     3841 tcgcgcgcag catcttcagc ctgtgcccaa gcgcaacacc gaggtccttg aggtgctggt
     3901 cggtcagctc ggtcaggaca tcggcgtcga tcgcgctttc gcgaaagacg gcttcgtatt
     3961 gactgaggcc cagaccgctt agccaggttc cgacatccat tttatggccg acccctcatt
     4021 ggcgcggggc gccgggccag acgaaatccg gcatccagaa ttgccctcaa ctgtaacctc
     4081 ctcgcatttc gtcgattttt ggtctgggcg caattcccgg gtgccgtgga atcgtagccg
     4141 gccggtcaaa cccgcaccga ttttttatat tttataccag aagttagcct tcgctgaatt
     4201 acacgcgcgg ggacgttatg gaaggcctct gatctcgcgg cggccggcgc tcgttgttgt
     4261 gtcaggggcc tcgcgggtcc ggcgtgggct ggcggacatc aggcagccgg accgccgtcg
     4321 cggacgatca gatcggctgg tagatctcgg atacgcagtt gtcgtcggct tcggcgcgcc
     4381 gccggtcgac gatggtgccg ccactgatcg tgacccgata ggtctcggtg cccagctgtt
     4441 tgcccgtgac cgatgtaagt tcggccctga cgcaggcgct ccagcccgag ccgtacggtt
     4501 cgtggtgcgg cggcgagacc cgcacctgac gcgggtacga cgtggcagtg aataccgaat
     4561 ccagttgttc gtggatcagc cggccgacat ccggtggcgg ttcgggcggc ggcgcctcgc
     4621 tggccgcggc gcgcatgaac tccggcaccg gcgagcggct gtcggcgatg ccgcagcctg
     4681 aaagcgcgat caccacgcca accatcaacg ccgaatgaac aatttgccgc agcatccggc
     4741 gatattatcc agccgcgggc ggcattgggg ggcggtcgcg gtcaattcgt gcgccttggc
     4801 gatcaaaaat tgctgaagcg tgagaagagc aagtttatcg tgcgccgcca cgacgattcc
     4861 ttgtttgtgg tgatgtggtt gcgtaagttt ggagcagaat gccagggaga gcggcgatga
     4921 agaccctcac ggttgcaacg gtgatgctgg cattgattgc aatgccggcg cacgctcaga
     4981 tgcagggcaa gcgccacacc ggcagcgacg aaaggaaaac cgaccagaag gaaaacaagg
     5041 tcgacgagaa ggcttacaag gcagcgctcg aaagaatccc ggattccaag gaaaaatatg
     5101 atccgtgggg catcgcgcgg cccgcggata ccaccaagaa accgaaatag ctggggtttg
     5161 tcggatcgag gtttgaacct ccgcgggata acaactgact tacttgttat cctggaggca
     5221 agcccatgct tcgttctgtc cttcgccatg cacttctggc cgccggtatt ttactgggct
     5281 cgcacgcggc gtttgccgag aatcgcgttg cgctcgtgat cggccagtca aactaccatt
     5341 ccgtgatcgc gcttcccaat ccggccaacg acgccagggc catgtcccgg ttgcttggcg
     5401 atgccggctt tgacgttacc agtgctgccg atctctcgca gaacgagatg cgtgaaaaag
     5461 tcagcgattt cgcggcaaaa gtggcggcga agggccctga caccgtcgca ctggtgttct
     5521 atgcgggcca tggcctgcag atagacggcg aaaatttcct ggtgcctgtc gacgtcgacc
     5581 cgaagcggga agccgacatt ccgctgcagg cgatacgcct caacgatgtt ctgaacaccc
     5641 tgacatcggt gccgagcaag acccgcatct tgctgctcga tgcctgccgc aacaacccgt
     5701 tccccgcgat caacgggacc gccgggcgcg ggctcgcgat cgtcgatgcc aagaccggcg
     5761 ctccaggcac gttcatgtcg ttttcgacgt ccccgggtgc cgaagccgag gatggcagcg
     5821 gcgccgacag tccgtacacg acagcactgc tttcggccgc caaggagccg gggctttcga
     5881 tcgaagacac cttcaagcgc gtgcgggttt cggtcaacaa ggtgaccgag gggcggcaaa
     5941 ccccctggga cagttcctcg ctgaccgacg acttcagatt tttttcgggg cccagcgcag
     6001 ccgcaccggc tccgaagccc gtggctgtga agcgcaccgt cgatcaatgg cgtcgtgaac
     6061 tgcagggcca gccggtcgag gccgccaatg aactgattgt ggccgacggc accgaagagg
     6121 cctatgaggc gtttgtcggg ctattcgcgc aacccccgtt cggcccgcag gcccgtgaat
     6181 ggctcgatcg tcaccgccga atggtggcgt ggaacaatgc ggtgattatc aacacggcgg
     6241 cggcgtatcg cgccttcctt gtgcaatacc ccgatagcga tctcaccgtg accgcacgca
     6301 agctcgagga tcgcctgcgc taccgtccga acattgcggg cagcctggcc ttggccgtcg
     6361 gcggcgcgcc gaacgggtca ggcggctcgg cgccggcctc gccgggcgga gcgcctttgc
     6421 cgaccaacgc ctcgctggcc gccccgacat gcccctgcgg tggcacgccg gccctgccac
     6481 tgaggaaggt cgatcttccg ccgaagaagc acgccgatcc ggagccgccc aagcgagcca
     6541 gcgtgcggcc gccgccgcgc gggccacgtg aaccaccacc cgatgacgat gtcgtggtct
     6601 accgccgttc cccgccgccc gactattacg aaccgtcgcc ggtaccctcg atcggcatcg
     6661 gaatagggat gggcatggga cggatcggcg gaggtggcgg cgattatcgc ggcggcggca
     6721 tggtgggccg aaggggcgga tactgattgc ggctcacagc attgcaaagg cgccggcttg
     6781 ttgtcggtgg cgctcgacag ggtgacgcgg ccaaagctca tggtgcgccc gaggcgcacc
     6841 acccgcgcat cggccaccac gtccgaggac gtgacggcgc gcatgaaatg ggtggtctga
     6901 tcgaccgtcg tcatcggccg gtatccgcgg ttggcggcaa ggatcgcgat caccatggcg
     6961 gtatcggcca gtgccatcag cgcctggccg cagacgatgc cgccatgcct gcacaggcgc
     7021 tcggagaacg ccatgcgcag gatcgcaccc ggctgccagt cggcaggcgc gcccggcggc
     7081 ggggcgcagt cgatactgcc gatcgacagc ccaaggtcct gcacccacgg cgcgaacacg
     7141 tcgccaagaa cgcgcctggc ttcctcgata tcaaattcct cggtctgtgg ttcatattgc
     7201 atgagactgc ggttcctcgt tcgacgcggc tgcggctatt ctcacgcatt ctaaagggca
     7261 ttggaaaggg acacggctgt tgcgcggctt ga
//
LOCUS       DATNOI010000136         4554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_308447,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000136 DATNOI010000000
VERSION     DATNOI010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308447"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1873)
                     /locus_tag="VN968_05155"
     CDS             complement(<1..1873)
                     /locus_tag="VN968_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXL28660.1"
                     /translation="MASTNRLSPIPHPPKKPVVGNMLSLDSSAPTQNLARLAKELGPI
                     FWLDMMGTPFVVVSGHDLVDELSDEKRFDKAVKGSLRRVRAVAGDGLFTAYTNEPNWS
                     KAHNILLQPFGNRAMQSYHPSMVDIAEQLVKKWERLNADDEIDVVHDMTALTLDTIGL
                     CGFDYRFNSFYREDYHPFVESLVRSLETIMLTRGLPFEGLWLRNRNRDLADDVAFMNK
                     MVDEIVAERRKNAEAAADKKDMLGAMMTGVDRATGEQLDDVNIRYQINTFLIAGHETT
                     SGMLSCTLYALLKHPEVLRKAYEEVDRVLGPDIDAKPTYQQVTQLTYITQILKEALRL
                     WPPAPAYGISPLKDETIGGKYKLKKNTFVLVLPLALHRDPSVWGPNPDAFDPQNFSRE
                     AEAAQPVNAWKPFGNGQRACIGRGFAMHEAALAIGMILQRFKLVDHNRYQMVLKETLT
                     IKPDGFKIKVRPRGEKDRGAYTGGTTTTAAASTATPTPRARTRPGHNTPLLVLYGSNL
                     GTAEELATRVADLAEVNGFATKLAPLDDSVGKLPEQGGVLIFCASYNGAPPDNATQFV
                     KWLGSDLPRDALSKVRYAVFGCGNSDWAATYQSIPRMIDEQLAAHGAKSVYARGEGDA
                     "
     gene            complement(2018..3013)
                     /locus_tag="VN968_05160"
     CDS             complement(2018..3013)
                     /locus_tag="VN968_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL28661.1"
                     /translation="MMEMPPLQFAQTNGIRMGYYEAGPNSDTPPVILCHGWPEIAFSW
                     RHQLKALAAAGIRVIAPDQRGYGATDRPEPVEAYDMEHLTGDLVGLLDHLKIDKAIFV
                     GHDWGGFIVWQMPLRHIDRVAGVVGVNTPHTNRAPADPIELFRKRFGEHMYIVQFQDP
                     SRSADKIFASRVEQTFDAFMRKPAPRKDTAPADAPTAGVGASPRLNLAFPQMIANYDA
                     KHDPRTPILSPEEKQVFVDTFTKSGFTGGINWYRNMSRNWQRAADIDHTVRVPSLMIM
                     AENDHVLPPSSADGMEKIVPDLEKYLVRGSGHWTQQEKPDEVSAKLIEWRKRRFG"
     gene            complement(3208..>4554)
                     /locus_tag="VN968_05165"
     CDS             complement(3208..>4554)
                     /locus_tag="VN968_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HXL28662.1"
                     /translation="PLDIDHPSWVEDDQFDIDRHIFRGSLPAPHDRATLERIVGWMHA
                     KLLNRARPLWEFYVFEGMKDNEIGLYSKMHHACIDGGAGAALTNMIYDVTPIPRNIEP
                     PTERAKVGQEPRDIAANLVDSYQQLWTKPFDAATAAKGIDLPRSGKSDLGSILFDNAM
                     FQIESAVKFAGSIPTMLKTVSDVVGKISDPKSRDSIASMVSPPTILNKSISSERSFAG
                     VSISLSEAKALAKQAGGKLNDVVLAISSGVVRHYLVERGALPAKSMTAAVPISLREEG
                     NTEANNQVFGMICSIATNIEDPKARLEAIIAQSTKSKEMSHPLRALMPQMSNLSMLGA
                     PIMVQILALLYSRSSLSDVLPPAANITVSNVPGPRQTLYAAGAELLHIFPVSISTHGL
                     ALNITVQSYRDQLDFGFIAGANIIPHVQVLCEMLPGEFAALQAAFAPPPDLTSAAS"
BASE COUNT          809 a         1396 c         1423 g          926 t
ORIGIN      
        1 gggcgtcgcc ctcgccgcgc gcgtagacgc tcttggcgcc gtgtgccgcc aattgctcgt
       61 cgatcatccg cgggatcgac tgataggtcg cggcccagtc gctgttgccg caaccgaaca
      121 ccgcgtagcg cactttcgag agcgcgtccc tcggcaggtc gctgccgagc cacttgacga
      181 attgcgtggc gttgtcgggc ggcgcgccat tgtacgacgc gcagaaaatc aggacgccgc
      241 cctgctccgg cagcttgccc acggaatcat cgagcggcgc gagctttgtc gcaaagccgt
      301 taacctcggc gagatcggcg acccgggttg ccagttcctc ggcggtcccg aggtttgatc
      361 cgtaaagcac caagagcggc gtgttgtgtc ccggacgggt ccgcgcccgc ggcgtcggcg
      421 tggcagtcga tgccgcggct gtcgtcgtag tcccgccggt ataagcgccg cgatctttct
      481 cgccacgcgg acgcaccttg atcttgaaac cgtcaggctt gatggtcagc gtttccttca
      541 gcaccatctg atagcggttg tgatcaacca gcttgaaacg ctgcaggatc ataccgatcg
      601 cgagcgccgc ctcatgcatc gcaaagccgc gaccgatgca ggcgcgctgg ccgttgccga
      661 acggcttcca cgcattgacg ggttgcgcag cctcagcctc gcggctgaag ttttgcggat
      721 cgaacgcgtc cgggttcggt ccccacaccg acgggtcgcg atgcaatgcc agcggaagaa
      781 ccaaaacaaa ggtgttcttc ttgagcttgt atttgccgcc gatggtctca tccttcagcg
      841 gcgagatgcc ataggcgggc gccggcggcc acagccgcaa ggcttccttc agaatctgcg
      901 tgatgtaggt gagctgcgtg acctgctgat aggtcggctt ggcatctata tcgggcccaa
      961 gcacccgatc gacttcctca taggctttcc tgagaacctc cggatgtttc aacagcgcat
     1021 agagcgtgca ggacaacatg ccgctggtgg tctcgtgccc tgcgatcagg aaggtgttga
     1081 tctggtagcg gatgttgacg tcatcgagct gctcgccggt ggcgcgatcg acgccggtca
     1141 tcatggcgcc gagcatgtcc ttcttgtccg cggcggcttc ggcgttcttg cggcgctcgg
     1201 cgacgatctc gtcgaccatc ttgttcatga acgcgacgtc atctgcgagg tcgcggttgc
     1261 ggttgcgcag ccacagccct tcgaacggaa ggccacgggt cagcatgatg gtctcaagcg
     1321 aacgcaccag cgactcgacg aacgggtgat agtcttctcg ataaaacgag ttgaaacggt
     1381 aatcgaaacc gcacaatccg atggtgtcga gcgtcagcgc ggtcatgtcg tgaacgacgt
     1441 cgatctcgtc gtcggcgttg agccgctccc acttcttcac cagctgctcg gcgatgtcca
     1501 ccatgctcgg gtgataggac tgcatggcgc ggttgccgaa cggctgcagc aggatgttgt
     1561 gcgccttgct ccagttcggc tcgttcgtgt acgctgtaaa cagtccgtcg ccggcaacgg
     1621 cccgcacgcg gcgcagcgac cctttcaccg ccttgtcgaa ccgtttctcg tcgctgagct
     1681 cgtcgaccag atcgtggccc gagacgacga caaatggcgt acccatcatg tcgagccaga
     1741 agatcggccc caattccttg gccagccggg ccagattttg cgtcggtgcg ctcgaatcca
     1801 gcgacagcat gttgccgacc accggttttt tcggcggatg cggaattggg ctcagcctgt
     1861 tcgtcgatgc cattgcgata ttttcccctg ccgttcgtgt gcaagaccgt catcctgagg
     1921 agcgcgcttc tgcgcgcgtc tcgaagaatg gccgcatgca cggtaccttg ccatccttcg
     1981 aggctcgccg aagtcggcga gcacctcggg atgacgctca tccgaatctt cgtttacgcc
     2041 attcgatcag cttggcgctg acctcatccg gcttttcctg ctgcgtccaa tggccgctgc
     2101 cgcgaaccag atacttctcg aggtcgggca cgatcttctc catgccgtcg gccgaagatg
     2161 gcggcagcac gtgatcgttt tcggccatga tcatcagcga cggcacccgc accgtatggt
     2221 cgatgtcggc cgcgcgctgc cagttgcgcg acatgttgcg gtaccagttg atgccgccgg
     2281 tgaagccgga ttttgtaaag gtatcgacaa acacctgctt ttcctccggc gacaggatcg
     2341 gcgtgcgcgg atcgtgtttg gcgtcataat tggcgatcat ctgcggaaaa gccagattga
     2401 gccgcggcga cgcgccaacg ccggcggtcg gcgcgtccgc cggcgcggtg tctttgcgtg
     2461 gcgccggctt gcgcatgaag gcgtcaaagg tttgttcgac ccggctggca aatatcttgt
     2521 cggcgctccg cgagggatcc tggaactgca cgatgtacat gtgctcgccg aagcgcttgc
     2581 ggaacagttc gatcgggtcc gccggggcac ggtttgtgtg cggcgtgtta accccgacga
     2641 cgccggcgac gcgatcgata tgccgcagcg gcatttgcca gacgataaag ccgccccagt
     2701 cgtggccgac gaaaatcgcc ttgtcgatct tgagatgatc gagaaggccg acgagatcgc
     2761 cggtcagatg ctccatatcg taggcctcga cgggctccgg ccggtcggtc gcgccatagc
     2821 cgcgctgatc gggcgcgatc acccggattc cggcggcagc cagcgccttg agctggtggc
     2881 gccatgaaaa cgcgatctcc ggccagccgt ggcagagaat gaccggcggc gtgtcgctgt
     2941 tcgggcctgc ctcgtaatag cccatgcgga tgccattcgt ctgggcaaac tgcagcggcg
     3001 gcatttccat catcgtcgaa tatcctgatc cgcggatcgt attctagcgc attttcgagt
     3061 tcggtgactc catccttcga gacggcgctt cgcgcctcct caggatgagg tcttggaccc
     3121 tcatggtgag gagcgcggca atgccgcgcg tctcgaacca cgaggccatg ggagatgagg
     3181 tcatgattcc acccatgcag atcggagcta gcttgccgcg ctcgtgagat cgggcggcgg
     3241 cgcaaaggcg gcttgcagcg cggcgaattc accgggcagc atctcgcaga gcacctgcac
     3301 gtggggaatg atgttggcgc cggcgatgaa gccgaaatcc agctggtcgc gatagctctg
     3361 cacggtgatg ttgagcgcaa gcccgtgagt cgagatcgac accgggaaga tatgcagcaa
     3421 ttccgcgccc gccgcgtaca gcgtctggcg cggccccggc acgttggaga ccgtgatgtt
     3481 cgccgccgga ggcagcacgt ccgacaggct tgagcggcta tacaacagcg ccaggatctg
     3541 gaccatgatc ggcgcgccaa gcatcgacag gttggacatc tgcggcatca atgcccgcaa
     3601 cggatgcgac atctccttcg acttggtgga ttgcgcgatg atcgcctcca gccgcgcctt
     3661 gggatcttcg atgttggtgg caatcgagca gatcatgcca aacacctgat tgttggcctc
     3721 ggtgttgccc tcttcgcgca acgagatcgg caccgccgcc gtcatcgatt tcgccggcag
     3781 cgcgccgcgt tccaccagat agtgcctgac cacaccgctc gaaatcgcca gcaccacgtc
     3841 attcagcttg ccgcccgcct gcttggccag cgccttcgcc tctgacagcg agatcgacac
     3901 gccggcaaag ctgcgctccg acgaaatcga tttgttcaga atagtcggtg gcgacaccat
     3961 actggcgata ctatcgcgcg atttcgggtc ggaaatcttt ccgaccacat cggagacggt
     4021 tttcagcatg gtgggaatgc tgccggcaaa cttcaccgcg gattcgatct ggaacatcgc
     4081 attgtcgaac aggatcgatc cgagatcgct ttttcccgag cgcggcagat cgatgccctt
     4141 ggcggccgtc gcggcatcga acggcttggt ccaaagctgc tgataggaat cgacgagatt
     4201 ggcggcgatg tcgcgcggct cttgcccgac cttggcgcgc tctgtcggcg gctcgatatt
     4261 ccgagggatt ggcgtcacgt cgtagatcat gttggtcagc gctgcgccgg ccccgccgtc
     4321 gatgcaggcg tgatgcatct tggagtaaag cccgatttcg ttgtccttca tgccttcgaa
     4381 gacatagaac tcccacaacg gacgcgcacg attgagaagt tttgcgtgca tccagccgac
     4441 gatgcgttcc agcgtcgcgc ggtcgtgggg tgcgggaagg ctgccgcgga agatatggcg
     4501 gtcgatatcg aactggtcgt cctcgaccca ggaggggtga tcgatatcga gcgg
//
LOCUS       DATNOI010000137         6966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_646301,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000137 DATNOI010000000
VERSION     DATNOI010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6966
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646301"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VN968_05170"
     CDS             complement(<1..392)
                     /locus_tag="VN968_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL28663.1"
                     /translation="MRNIMKSAAVLSAVLASAPAIAGWEPTKPVEIVVAAGAGGASDQ
                     MARMMQAAIQKNNLMKQPMVVSLKGGASGAEALMYMKSSEGDANKVMIAYSLIYMLPL
                     SAKIPFNWRELTPVSVVALDQFVLWDNA"
     gene            complement(767..1564)
                     /locus_tag="VN968_05175"
     CDS             complement(767..1564)
                     /locus_tag="VN968_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXL28664.1"
                     /translation="MNAPIPATEDLIYLVEDGIARLTFNRPQARNALTFAMYEQMAAI
                     CETVNADRSIKAMILTGTGDKAFASGTDISQFRAFKTSQDALDYEARIDRVLGMLEQV
                     RVPTIGAIAGACTGGGAGIAACCDIRIGTHAARIGFPIARTLGNCLSMSNISRLVSLI
                     GPARTKDLIFKARLVEAPEALALGLLNEVVPDVETLQRRANETAQLVAGHAPITLEVT
                     KEAVRRIRRTLSRDEGEDLILRAYMSEDFREGMDAFLNKRTPNWKGK"
     gene            complement(1612..2817)
                     /locus_tag="VN968_05180"
     CDS             complement(1612..2817)
                     /locus_tag="VN968_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXL28665.1"
                     /translation="MTLHTGRHFLQIPGPTNVPDRVLRAMDMPTLDHRGPEFAELGHA
                     VLAGCQRIFRTQQPVIIYPASGSGAWEAAIVNTLSPGDKVLMAETGQFAVLWLGIAEK
                     FKLDVEFLPTDWRRGADVEAIEAKLAADRLHKIKAVLVVHNETSTSCVTHPLEVRKAL
                     NRTNHPALLMVDTISGLASLEYEHDAWGIDISVGGSQKGMMLPPGLSFNAISEKALAA
                     SKANSSLRSYWDWHDMIAINKLGTFPYTPATNLLFALKEAIAMLEEEGLDNVFARHQR
                     HGAATRAAVKAWGLDLVCLDPHAYSAALTAVMVPDGHDADALRKVILENFDMSLGTGL
                     GKLKGKAFRIGHIGHFNDLMLMGTLSGVEMGLDLAKVPHRNGGVLAAMDVLKRRDVVS
                     MPTSKAAVA"
     gene            complement(3013..4302)
                     /locus_tag="VN968_05185"
     CDS             complement(3013..4302)
                     /locus_tag="VN968_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL28666.1"
                     /translation="MRLRFKATHVVLAMLCVMYFITYIDRVNISTAASEIQKELHLSN
                     TQLGLVFSAFAYPYLLFQVIGGWVGDRFGPRKTLFWCGLIWAVATILTGLVTSLLTLF
                     IARVALGFGEGATFPTATRAMQYWTPMGRRGFAQGITHAFARLGNAITPPLIAVLMAW
                     LTWRGSFIALGLVSLLWGVVWALYFRNEPRDHPSITEQELASLPPRPVGTRPEVPWGP
                     LLRRMWPVTLTYFCYGWTLWLYLNWLPLFFKNSYSLDIKNSALFASGVFFAGVVGDSL
                     GGILSDRILKKTGSVRFARLSVTITGFAGALLSLFPILFIHDITVVALCLSGGFFFAE
                     ITIGPMWSVPMDIAPKYSGTAAGLMNTGSALAAIVSPLVAGYVIDVTGNWYLPFLMSM
                     GLLLLGGFSAFLMHPETPFEEEEGGVIPIAGRPVAAR"
     gene            complement(4376..5578)
                     /locus_tag="VN968_05190"
     CDS             complement(4376..5578)
                     /locus_tag="VN968_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HXL28667.1"
                     /translation="MTQGRHFLQIPGPSPVPDRVLRAMDMPVIDHRSSQFAELAKTVF
                     EGCQKIFQTAGPVIIFPSSGTGAWEAAIVNTLSPGDKVLMVETGHFATLWRQMAARWG
                     IEVDFVPGDWRHGADPADIEARLAQDSAHAIKAVMVVHNETSTGVTSRIGGIRAAMDK
                     VGHPALLLVDTISSLGSVDYRHDEWKVDVTVSCSQKGFMLPPGLGFNAISEKARAAAK
                     TNKMPRSYWDWEEMLKPNAAGFFPYTPATNLLYGLREAISMLLEEGLEAVFARHQRLA
                     AATRAAVAHWGLEVLCLEPSEYSPVLTAVLMPPGHDADQFRKTALDTYNMSLGSGLSK
                     LAGKVFRIGHLGECNELTLMGALSGVEMGLAAAGVPHRAGGVDAAIKSLEAPTQSNSP
                     SHLKVVKT"
     gene            5692..6429
                     /locus_tag="VN968_05195"
     CDS             5692..6429
                     /locus_tag="VN968_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXL28668.1"
                     /translation="MKRLIPSDATSTELGADSTPDREDQSLHDEILTRLRDHIVEGNI
                     PDGDRMPERQLCEMLGISRTPLREALKVLASEGLVELLPNRGARVRQLSEHDLAELFD
                     VMGGLEALAGRLACENITDAGIAEIERLHYEMYGFYLHRDMHGYFRVNQLIHLKIVEA
                     SRNATLQSTYANFAGRIRRIRYAANFARKRERWGEAMREHETILDALRRRAGSELSDI
                     LFSHLRNKRTAAAEHLKADDAAAPEPA"
     gene            6572..>6966
                     /locus_tag="VN968_05200"
     CDS             6572..>6966
                     /locus_tag="VN968_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXL28669.1"
                     /translation="MRLEDRLARETTGDVLFDPFSRGRYATDASFYQIMPAGVVVPRT
                     IDEALRALSICRDDGRTVTPRGGGTSQCGQTVNDGIVIDFSRHLNRIVSLDVESRTCV
                     VEPGIVLDDLNRQLKQHGLWFPVDVSTASR"
BASE COUNT         1335 a         2241 c         2140 g         1250 t
ORIGIN      
        1 ctcgcattgt cccacagcac gaactggtcg agcgcgacca ccgacaccgg cgtcaactcg
       61 cgccagttga acggaatctt cgccgacagc ggcagcatgt agatcagcga ataggcgatc
      121 atcaccttgt tggcgtcgcc ctcgctggat ttcatgtaca tcagcgcttc ggcgccggac
      181 gcgccgccct tgagcgagac gaccatcggc tgcttcatca gattgttctt ctggatcgca
      241 gcctgcatca tccgcgccat ctggtcggag gcaccgccgg ccccggccgc gacgacgatt
      301 tcaacgggtt ttgtgggttc ccagccggcg atcgctggcg cgctcgccag cacggccgac
      361 agcacggcgg cggatttcat gatatttcgc aggtgttttc tcccctatgt ctttgatgag
      421 cgacaagtcc ggacatggtc tgcggggcgc ccttgcgcgc ggcaacgacc ggttgagttc
      481 aagcaggccc cgaccgcact catgatgact ttcgcatgag attgcaagcc gttaccgcga
      541 actccgtcag ttcagcaacc gattgaaaga cacccttggc gtcacggcaa catcggcgcc
      601 gcctgcagcg aactcgcggt cgcaataaac ccgtggtcgg gatgtcgaac acgaatgccg
      661 cacgcgcaac gcgcgccgcc attcgagacg cgcgaactgg tgggagagcc cgcgccgctc
      721 ggccgccgca ccagcagcac ggaggtcgct atcgcttgtt gcgaacctat ttgcccttcc
      781 agttcggcgt gcgcttgttg aggaaggcat ccattccctc gcggaagtcc tcgctcatat
      841 aggcgcgaag gatcaaatcc tcgccctcgt cgcgcgacag tgtccgccgg atccggcgca
      901 ccgcctcctt ggtgacctca agcgtgatcg gcgcatggcc ggcaaccagt tgcgcggtct
      961 cattggcacg gcgctgcagc gtctcgacat ccggtaccac ttcgttcagc aaaccgagtg
     1021 ccagcgcctc gggggcttcg accagccggg ccttgaaaat cagatccttg gtccgcgccg
     1081 ggccaatcag cgaaaccagc cggctgatgt tggacatcga caggcaattg ccgagcgtcc
     1141 gcgcgatcgg aaagccgatt cgggctgcat gggtgccgat ccggatatcg caacaggcgg
     1201 cgatccccgc cccgccgccg gtgcaggctc cggcgatggc cccaatagtc ggcacccgga
     1261 cctgttccag catccccaaa acccgatcga tccgcgcctc atagtcgagc gcatcctgcg
     1321 aggtcttgaa ggcgcggaat tgcgagatgt cggtgccgga tgcgaacgcc ttgtcgccgg
     1381 tgccggtcag gatcatcgcc ttgatcgagc ggtcggcgtt gacggtctcg cagatcgccg
     1441 ccatctgctc gtacatcgca aacgtcagcg cattgcgcgc ttgcggccgg ttgaaggtca
     1501 gccgggcgat gccgtcctcg acgagataga tcaggtcttc ggtcgctggt atcggggcgt
     1561 tcatggtacg ctctttctgt tcttgccggt gttgcgcagc atagcatcag ctcaggcgac
     1621 ggcagccttt gatgtcggca tcgacaccac gtcgcgtcgt ttcaggacat ccatggcggc
     1681 gagcacgccg ccgttgcgat gcggtacctt tgcaagatcc aggcccatct cgacgcccga
     1741 cagcgtcccc atcagcatca gatcgttgaa atgtccgata tggccgatcc ggaacgcctt
     1801 gcccttgagc tttccgaggc cagtgcccag ggacatgtcg aagttttcca ggatcacctt
     1861 gcggagggcg tcggcatcgt gtccgtccgg aaccatgacc gcggtcagtg ccgccgaata
     1921 cgcgtgcgga tcgaggcaca cgaggtccag accccatgcc ttgaccgccg cgcgggtcgc
     1981 cgcgccatgg cgctggtgcc gcgcgaacac gttatccagc ccctcttcct caagcatcgc
     2041 aatggcttcc ttgagcgcaa acagcaggtt tgtcgccggc gtgtaaggga atgtgccgag
     2101 cttgttgatc gcaatcatgt cgtgccagtc ccagtaggac cgcaacgacg agttggcctt
     2161 ggaggccgcc agcgcctttt ccgagatcgc attgaagctc agccccggcg gcagcatcat
     2221 gcccttttgc gacccgccga cggatatgtc gatgccccag gcatcgtgtt cgtattcgag
     2281 cgaggcgagc ccggagatgg tgtcgaccat cagcagcgcc gggtggttgg tgcggttgag
     2341 ggccttgcga acttcgagcg gatgcgtgac gcaactggtc gaggtctcgt tgtgcaccac
     2401 cagcacggcc ttgatcttgt gcaggcgatc ggcggcaagc ttcgcctcga tcgcttccac
     2461 atcggcgccg cgccgccagt cggtgggcaa gaattcgaca tcgagcttga atttctcggc
     2521 aatgccgagc cacaacaccg caaactggcc ggtctcggcc atcagcacct tgtcgccggg
     2581 cgacagcgtg ttcacgatcg cggcttccca ggcgccgctg cccgatgccg gatagatgat
     2641 caccggctgc tgggtccgga agatgcgctg gcaacctgcg agcacggcat gaccgagttc
     2701 ggcgaattca ggaccgcgat gatccagggt cggcatgtcc atggcccgca gcacccggtc
     2761 gggcacattg gtcggtcctg gaatctgtag aaaatgcctt cccgtgtgca aggtcatctg
     2821 gcgtccttcc cggtcttgtc ttggttcgcg gctcttggtt cgcggcgctc gaatatcact
     2881 attcggcggg cttgtcccct gcccggcggg cgccgtcaat gcataagagg ccatgctttg
     2941 cctggcaaag cccaggaaac caggctttgc cgtgcaaagc ccccggggat ccctgacatg
     3001 cgaggggctg gtttatctcg ccgcgacggg tcgtccagcg atcggaatca cgccgccctc
     3061 ttcttcctcg aacggcgtct caggatgcat caggaacgcc gaaaaccctc cgagcagcag
     3121 caggcccatc gacatcaaga acggcaaata ccagttgccg gtcacatcga tcacgtaacc
     3181 ggccaccaaa ggcgaaacaa tcgccgccag cgccgagccg gtattcatca ggccggcggc
     3241 ggtaccggaa tatttcggcg caatgtccat cggcaccgac cacatcgggc cgatggtgat
     3301 ctcggcgaag aaaaagccgc ccgacaagca cagcgccacg accgtgatgt cgtgaatgaa
     3361 caggatcggg aacagcgaca gcaaggcgcc ggcgaagccc gtgatcgtca cgctcagccg
     3421 cgcaaagcgg acgctgccgg tcttcttgag aatgcggtcc gacaggattc cgccgagact
     3481 atcgccgacc acgccggcaa agaacacgcc cgaggcgaat agcgcggaat tcttgatgtc
     3541 gagactatag ctgttcttga agaacagcgg cagccagttg aggtaaagcc acagggtcca
     3601 gccatagcaa aaataagtca gcgtgaccgg ccacatccgc cgcaacaacg gcccccatgg
     3661 cacctcgggg cgcgtaccga cggggcgtgg cggcaacgaa gccagctcct gctcggtaat
     3721 ggaaggatga tcgcgcggct cgttgcggaa atacagcgcc cagaccacac cccacagcag
     3781 gctgaccagt cccagcgcga taaacgagcc gcgccaggtc agccaggcca tcaacaccgc
     3841 gatcaacggc ggcgtgatgg cgttgccgag ccgggcaaag gcgtgggtga ttccttgcgc
     3901 gaacccgcgc cgccccatcg gagtccagta ctgcatggcg cgggtggcgg tcgggaaggt
     3961 cgcgccttcc ccgaagccga gcgcgacgcg ggcaatgaac agggtcagca ggctggtcac
     4021 aaggccggtc aggatggtgg caacggccca gatcagcccg caccagaaca aggtcttgcg
     4081 cggcccgaag cggtctccga cccagccgcc gatcacctgg aacagcagat agggataggc
     4141 gaaggccgag aacaccagac cgagctgggt attgctcaga tggagttcct tctggatttc
     4201 gctggccgcc gtcgaaatgt tgacccggtc gatataggtg atgaagtaca tgacgcagag
     4261 catcgccagc acgacatggg tggccttgaa ccgaagccgc atttttgtcc ttcccgagtt
     4321 cgctttgttt atggcttcaa cggcagcacc gtcgccaagg tcgtcttcga ccctgtcagg
     4381 tcttgaccac cttcagatgc gacggggaat ttgattgggt cggtgcttcc agcgacttga
     4441 tcgccgcgtc gacgccgcct gcccgatgcg gcacgcccgc ggcggcaagg cccatctcga
     4501 cgccggacaa ggctcccatc aaggtgagtt cattgcactc gccgaggtgg ccgatgcgaa
     4561 acaccttgcc cgcgagcttg ctcaggccgg aaccgagcga catgttgtag gtgtcgagcg
     4621 cggtcttgcg gaactggtcg gcgtcatgtc ccggcggcat cagcacggcg gtcagcaccg
     4681 gggaatattc cgatggttcg agacacagca cttcgaggcc ccaatgcgcg acggcggcgc
     4741 gggtggcggc cgcgagacgc tggtgacggg cgaacaccgc ctccagcccc tcctcgagca
     4801 gcatcgagat ggcttcgcgc aggccgtaaa gcagatttgt ggccggcgtg taaggaaaga
     4861 agccggccgc gttgggcttg agcatctctt cccagtccca atacgaccgc ggcatcttgt
     4921 tggtcttggc ggccgctctg gccttctcgg agatggcgtt gaagccgagc ccgggcggca
     4981 gcatgaatcc cttttgcgaa caactgacgg tgacgtcgac cttccactcg tcgtgccggt
     5041 aatcgaccga gccgagcgag gagatcgtat ccaccagcag cagcgccgga tggccgacct
     5101 tgtccatcgc ggcgcggatg ccgccgatcc ggctggtgac gccggtggag gtctcgttgt
     5161 gcaccaccat gaccgccttg atggcatggg ccgaatcctg cgccagcctg gcctcgatat
     5221 cagcgggatc ggcgccgtga cgccaatcgc ccggcacaaa atcaacctcg atgccccagc
     5281 gcgccgccat ctgccgccac agcgtggcaa agtggccggt ctcgaccatc aggaccttat
     5341 cgccaggcga cagcgtattg acgatcgccg cctcccaggc gccggtgccc gacgagggga
     5401 agatgatgac cgggcccgcc gtctggaaga ttttctggca accctcgaat accgtcttgg
     5461 cgagctcggc gaattgggag ctgcggtggt cgatgaccgg catatccatg gcgcgaagca
     5521 cgcgatccgg aaccggactt gggccgggaa tttgcagaaa atggcgtccc tgggtcatga
     5581 attcctcgct gggctaatgg ccatattgca ttcaattatt aacgcttcaa ccaaaatatt
     5641 gtttacattt tttccatcga ccgcctgccc gccaacggat atgttcccgt catgaagcgc
     5701 ttgatcccca gcgacgctac ttcgaccgag cttggcgccg actcgactcc cgaccgggag
     5761 gaccaatccc tgcatgacga gatcctgacg cggctgcggg accacatcgt cgaaggcaac
     5821 attccggacg gcgaccggat gccggaacgc cagctgtgcg agatgctggg catctcgcgc
     5881 acgccgctgc gtgaggccct gaaggttttg gcgtccgagg ggctggtcga gttattgccc
     5941 aaccggggcg cccgcgtgcg gcaattgagc gagcacgacc ttgccgaact gttcgacgtc
     6001 atgggcgggc tcgaagctct cgccgggcgg cttgcctgcg aaaacatcac cgacgccggg
     6061 atcgccgaga tcgagcggct gcactacgag atgtatggtt tctacctgca ccgggacatg
     6121 cacggctatt tccgggtcaa ccagctgatc cacctgaaga tcgtcgaagc ctcgcgcaat
     6181 gccacgctgc agagcactta cgccaatttc gcaggacgga tccgccggat tcgctacgcc
     6241 gccaattttg cccgcaagcg tgagcgctgg ggcgaggcga tgcgtgaaca cgagaccatc
     6301 ctggatgcgc tgcgccgccg cgccgggagc gagctcagcg acatcctgtt cagccatctg
     6361 cgcaacaagc ggacggccgc cgccgaacat ctgaaggccg acgatgccgc cgcgcccgag
     6421 ccagcctgaa attccttaag agttcgaacc acaattttga attcaaaatg taaaacgctc
     6481 ctgagcgaat tgcatttctg actaggcgcc gttaccagca gctgtgttgc cggtagcgac
     6541 cagcgagagt gcgatgtcca acagaggctc catgaggctt gaagaccggc tggcgcgcga
     6601 gaccaccggc gacgtcctgt tcgacccgtt cagccgcggc cgctatgcca ccgacgcgtc
     6661 gttctaccag atcatgcccg ccggcgtggt ggtgccgcgc accatcgacg aagcgctgcg
     6721 ggccctgtcg atctgccgcg acgacggcag gaccgtcacg ccgcgcggcg gcggcacgtc
     6781 gcaatgcggc caaaccgtca atgacggcat cgtcatcgat ttctccagac acctcaaccg
     6841 catcgtctcg ctggacgtcg aaagtcgaac ctgcgtggtc gaaccgggga tcgtgctcga
     6901 cgacctcaac cggcaactca aacaacatgg gttgtggttc ccggtcgatg tgtccaccgc
     6961 ctcccg
//
LOCUS       DATNOI010000138        10542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_646153,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000138 DATNOI010000000
VERSION     DATNOI010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10542
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646153"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..2295
                     /locus_tag="VN968_05205"
     CDS             88..2295
                     /locus_tag="VN968_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HXL28670.1"
                     /translation="MAWGRKKGGGRKEPRFGPAASLAELRLSPEDRVTVGDEDDEDET
                     PKKPPAKRKARDADDDAPRERKPRPTPVSRTRKSKTRSPIGISRLFYWAAVLGLWAAI
                     AVVGVIIWVGAHLPPIQSLEIPKRPPTIQITGFDGSVLATRGELAGANVALKDLPPYL
                     PKAFIAIEDRRFYSHYGVDPVGIARAAVANILHRGVSQGGSTLTQQLAKNLFLTQERT
                     LQRKLQEVELAFWLERKHSKSEILELYLNRVYFGSGAYGVEAAAQRYFGKSAKNVTIA
                     EAAMLAGLVKSPTRLAPNRNPEGAEKRAQTVLSAMADAKFITDAQAQASIGHPSYHVK
                     AAGAGTVNYVADWIGEVLDDLIGPVEQSIVVETSIDPKLQNVAEASIIDELAAKSVKF
                     NVSQGALVAMTPDGAVRAMIGGRNYAESQYNRAVTAKRQPGSAFKPFVYLTAIEAGLT
                     PETIRQDAPLDVKGWKPENYSHEYLGPVTLTQALAMSLNTVAVRLGLEVGPKNVVRTA
                     HRLGIASKLETNASIALGTSEVSLTELVGAYAPFANGGQGVTPHVVTRIRTSEGKVLY
                     ARLPDQLGQVIEPRHVAMMNTMMQETLLSGTARKAEIPGWMAAGKTGTSQDFRDAWFV
                     GYTANLVTGVWLGNDDNSPTRKTTGGGLPVEVWTRCMRAAHQGVPVAALPNAQAGGLF
                     SNLVQTASQVSAPSAPTAAAQATSAGARPTRTSVRPDTTAGLDGWLMDRLFGR"
     gene            complement(2507..3346)
                     /locus_tag="VN968_05210"
     CDS             complement(2507..3346)
                     /locus_tag="VN968_05210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HXL28671.1"
                     /translation="MICFCAERFHWRQIWQNPGEFLTSGLTDMATRALLYRRPAEPSR
                     LLVKHGSQIYSIRLRRHRRARRYTLRIHPSDREAILTMPPRGTIADAKDFAQRHGGWI
                     AARLGRLPKAAPFHPGTVVPLRGLPYRIVHRAGLRGTVWTEIRDSGERILCVAGGVEH
                     IDRRVHDFLKREARKDLQKAAQSYAQALGVKVKRLSIRDQSSRWGSCTSAGSLSFSWR
                     LILAPPYVLDYLAAHEVAHLVEMNHSARFWRIVAKVCGHVERAKTWLDTYGNDLHRYG
                     IQD"
     gene            complement(3576..4904)
                     /gene="phaZ"
                     /locus_tag="VN968_05215"
     CDS             complement(3576..4904)
                     /gene="phaZ"
                     /locus_tag="VN968_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HXL28672.1"
                     /translation="MPIGEFGGAPPLVAEGSPALTTPMYWMYEMAQASLNPARAVTDA
                     TKLLFQNPLNPWSRTEFGKSVAAGCELFERTTRRYGKPEWGLNDTEVNGVRTPVEIRV
                     VWEKPFCRLLYFDRKLTRPLRSPQPRVLIVAPMSGHYATLLRGTVEAFLPTHEVYITD
                     WSDARMVPLTEGRFDLDDYVDYIIEMLHTLGRNMHVIAVCQPSVPVVAAVSIMEAERD
                     PLVPVSMTLMGGPIDTRRNPTAVNNLAAERGIDWFRSHVITKVPFPHPGVMRDVYPGF
                     LQLSGFISMNLDRHTDAHKALFKNLVKGDGDLVDKHRDFYDEYLAVMDLSAEYYLQTV
                     DTVFVKHALPKGEMTHRGKRVDPSKITRVALMTVEGENDDISGLGQTEATHALCSAIP
                     DHRRVHYVQKGVGHYGVFNGSRFKSEIVPRISDFMVSAANATPSLSAAAE"
     gene            complement(5040..5843)
                     /locus_tag="VN968_05220"
     CDS             complement(5040..5843)
                     /locus_tag="VN968_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL28673.1"
                     /translation="MHRGIAPHRINAMILRYWYLLMSSWPRLLELIYWPALQIITWGF
                     LQNYISQTSGFFARAGGTLIGAVILWDILFRGQLGFSISFLEEMWARNLGNLMMSPLK
                     PIEFLIALMVMSLIRLAIGVIPMTGLAMIFFDFNFYGIGLPLIAFFCNLIFTSWSLGI
                     FVSGLVLRNGLGAESIVWTLMFGVMPLACVYYPVTVLPHWLQYVAWALPPTHVFEGMR
                     ALLIDHEFRADLMVEALLINAVLLIASFAIFLALLRSARQHGSLIQSGE"
     gene            complement(5855..6649)
                     /locus_tag="VN968_05225"
     CDS             complement(5855..6649)
                     /locus_tag="VN968_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28674.1"
                     /translation="MVNNDAAPPKLAAIDPAGSAAIEVAHLVKLYKTTRAVDDVSFRI
                     ARGSVTGLLGGNGAGKTTTIAMIMGLVLPTSGRIQVLGCSMPEQSAEVLGRMNFESPY
                     VDMPMRLTVRQNLTIFGRLYAVANLRERIAKLAADLDLNEFLDRPNGKLSAGQKTRVA
                     LAKALINQPELLLLDEPTASLDPDTADWIRQHLERYRKTQGATILLASHNMLEVERMC
                     DRVIIMKRGRIEDDDSPDGIMARYNRATLEEVFLDVARGRIQEGAS"
     gene            6819..8144
                     /locus_tag="VN968_05230"
     CDS             6819..8144
                     /locus_tag="VN968_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HXL28675.1"
                     /translation="MSDVAASDFRHPRRHVRLDTILRLRWLAALGQLAAIFIVIQGLE
                     FDVPVIPCVTIVGLSALLNLVLQMAFNPMQRLEPAYAAALLALNIVELAGLLYFTGGL
                     QNPFSFLFLAPVLISATALPIRLTIALGALAIACASALVFFHEPLPWDGDEPLVLPPI
                     YLVGVWLSIVVAIGVTSLYAFQVTEEARKLSDALAATELVLTREQHLTQLDGLAAAAA
                     HELGTPLSTIFLISRELERAVPDASPLASDLKTLREQAQRCRDILAKITQLSSTGAPF
                     DLMPLSTLIEEVVAPHRDFGVQIKVRIAVAGTREPVGTRNPAILYGVGNILENAVDFA
                     RATVEVNAWWNTETVEIVISDDGPGIAPDILKRIGEPYLSRRRGADEAQGDHHGLGLG
                     VFIARTLLERTGAKVSFTNRTFPDHGAVVQIAWPRSRFEASETEAESIV"
     gene            8249..8803
                     /locus_tag="VN968_05235"
     CDS             8249..8803
                     /locus_tag="VN968_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HXL28676.1"
                     /translation="MTAIAELIDHADRSLLIVEDDKAFLERLSRAMETRGFTVTSCDS
                     VSDGLAQIGKAAPAFAVVDLRLGDGNGLDVVSALKRKRPDARAIVLTGYGNIATAVTA
                     VKMGAVDYLSKPADADDVVAALLASGTEKSELPINPMSADRVRWEHIQRIYEMCNRNV
                     SETARRLNMHRRTLQRILAKRAPR"
     gene            9084..10157
                     /locus_tag="VN968_05240"
     CDS             9084..10157
                     /locus_tag="VN968_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL28677.1"
                     /translation="MVAAIERISKRKDASSQPEGSDKASDISALIARLTTEVDQIACE
                     KTKSIQQITNQMKMLALNALIESSRAGALGAGFAVVAQEVRSVGQKVEDIARELETEL
                     TGRTANLAQSIEKMMERSRGERMVDLALNAVELIDRNLYERTCDVRWWATDSAVVDCA
                     ASPGAANVSHVAERLAVILRAYTVYLDLWLCDLNGNVLASARSDRYDVKGASVARMPW
                     FSDALKLRSGDDYVAGDIERQPLLGNAQIATYCASVREGGKANGKPIGVLAIHFDWEA
                     QARAIVEGIRVDDAKARVLLVDSKFRVIASSDGQGLLTERVPLKGGRSGFYKDGSGPQ
                     VAFHLTPGYETYKGLGWFGVILR"
     gene            complement(10254..>10542)
                     /locus_tag="VN968_05245"
     CDS             complement(10254..>10542)
                     /locus_tag="VN968_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HXL28678.1"
                     /translation="EIKSSIEDLRADQKWQDYRMHCDRLFFAFTQDLPCEIFPGQTGL
                     IVADAYGAHMHCEAPEHRLPAPTRKAMTVRFAIAAAQRINRLVDPQGHGEF"
BASE COUNT         2033 a         3324 c         3329 g         1856 t
ORIGIN      
        1 tcgcggcatc agcattaatc atcacttaac ccagccttaa accccacccg ctagtctctc
       61 gcatctgtgg cgcgggcgtt gttgcgtatg gcgtggggac gaaaaaaggg cggcggacgc
      121 aaagagccgc gattcgggcc cgccgcgtcg ctggcggaac tgcgtctcag tccggaggat
      181 cgcgtcaccg ttggcgacga ggacgacgaa gatgagacgc cgaaaaagcc gcctgccaaa
      241 cgcaaggccc gcgatgcgga tgacgacgct ccgcgcgagc gaaagccgcg tccgacaccg
      301 gtgagccgca cgcgcaaatc caagacacgc tcccccatcg gcatttcgcg gctgttctat
      361 tgggccgcgg tgctcggtct gtgggccgcc atcgccgtgg ttggcgtcat catctgggtg
      421 ggcgcgcatc tgccgccgat ccagtcactt gaaatcccca aacgcccgcc gacgatccag
      481 atcacgggct tcgacggcag cgtgctggcg acgcgcggcg aattggccgg cgccaatgtc
      541 gcgctgaagg atctgccgcc ctatctgccg aaagccttca tcgcgatcga ggaccgccgt
      601 ttctattcgc attacggcgt cgatccggtc ggcatcgcgc gtgcggcggt cgccaatatc
      661 ctgcatcgcg gggtttcgca gggcggctca acgctgaccc agcagctcgc caagaatcta
      721 tttctgacgc aggagcgcac cctgcaacgg aaattgcagg aggtcgaact cgcgttctgg
      781 ctggagcgca agcattccaa gtcggagatt ctcgaactct acctcaaccg ggtatatttc
      841 ggttccggcg cctatggcgt cgaggcggcc gcgcagcgct atttcgggaa atccgcaaag
      901 aacgtcacga tcgcggaggc cgcgatgctt gcggggctgg ttaaatcgcc gacgcggctg
      961 gccccgaacc gcaatccgga aggtgccgag aaacgcgccc agaccgtgct gtccgcgatg
     1021 gcggacgcca aattcataac cgatgcacag gcgcaggcct cgatcggaca tccgtcttat
     1081 catgtgaagg cggccggcgc cggcaccgtc aattatgtcg cggactggat cggcgaagtg
     1141 ctcgacgatc tgatcggccc ggtcgagcag agcatcgtgg tcgaaacctc gatcgatccg
     1201 aaattgcaga acgtggctga agcttccatc atcgacgaac tcgcggcgaa aagcgtaaaa
     1261 ttcaatgtca gccagggcgc gctggtggcg atgacgccgg acggcgccgt gcgcgccatg
     1321 atcggcggcc gcaattacgc cgagagccag tacaaccgcg cggtcaccgc aaaacgccag
     1381 ccgggctcgg cgttcaagcc gttcgtgtat ctgaccgcga tcgaagccgg tctgacgccg
     1441 gagacgatcc ggcaggacgc gccgctcgac gtcaagggct ggaagcccga aaactacagc
     1501 catgaatatc tcggcccggt gacattgacg caggcgctgg cgatgtcgct caataccgtg
     1561 gcggtgcggc tgggactcga agtgggaccc aaaaacgtgg tgcgtaccgc gcaccggctc
     1621 ggcattgcat cgaagctcga gaccaatgcg tcgattgcat tgggcacctc cgaagtctcg
     1681 ctcaccgaac tggtcggcgc ctacgcgccg ttcgccaatg gcggccaggg cgtcaccccg
     1741 catgtcgtga ccaggattcg caccagtgag ggcaaggtgc tgtacgcccg gctcccggac
     1801 cagcttggcc aggtgatcga gccgcgccat gtcgcgatga tgaacaccat gatgcaggag
     1861 accctgctga gcggcaccgc gcgcaaggcc gagatcccgg gctggatggc cgccggcaag
     1921 accggcacca gccaggattt ccgcgacgcc tggttcgtcg gctacaccgc caatctcgtg
     1981 accggcgtct ggctcggcaa tgacgacaat tcaccgacca ggaagacgac cggcggcggg
     2041 ttgccggtcg aggtctggac ccgctgcatg cgcgcggccc atcagggtgt cccggtcgcg
     2101 gcgctgccga acgcgcaagc gggcggtttg ttctcgaatc tcgtgcagac cgcctcgcag
     2161 gtcagcgcac cttccgcgcc cacggccgcc gcgcaagcta cgagcgctgg ggccagaccg
     2221 acgcgaacct cggtacgccc ggacacaacc gccggactgg acggctggct gatggaccgg
     2281 ctgttcggac gatagtgggg tgaactccgg atgctgcgcc gggcactgcg cttcatcatt
     2341 tttggtcgtc gtgctccgcc accgggtctc gccttcggcg agcccgatga caggctccag
     2401 cggagtatcc ggtacgccgc agcgtttcga cttgatcact ggcgctctag aataccgggt
     2461 ccacgcattc cgcggggacg acgaaggcga gaatgagacc gctacctcaa tcctgtatgc
     2521 cgtagcgatg caggtcgttg ccataggtat cgagccaggt cttggcgcgc tcgacatgac
     2581 cgcagacttt cgcgacaatc cgccagaacc gcgccgaatg gttcatctcg acgagatgcg
     2641 ccacctcatg ggcggcgaga tagtccagca catagggcgg cgcgaggatc agccgccacg
     2701 agaacgacag cgagccggcc gaagtacaag aaccccagcg gctcgactga tcgcggatcg
     2761 acagccgctt gaccttgacg ccgagcgcct gcgcatagga ctgcgccgcc ttctgcaggt
     2821 ccttgcgcgc ttcgcgcttg agaaagtcgt ggacccgacg atcgatatgc tcaacgccgc
     2881 cggcgacgca gagaatccgc tcgccgctgt cgcggatttc cgtccacacc gtgccgcgca
     2941 agcccgcccg atgcacgatc cgatacggca gcccacgcaa cggcaccacg gttcccggat
     3001 gaaacggcgc ggccttcggc aaccggccga ggcgcgcggc gatccagccg ccgtgacgct
     3061 gcgcgaaatc cttggcgtcg gcgatggtgc cgcgcggcgg catggtcagg atggcttcgc
     3121 ggtcgcttgg atgaatgcgc agggtgtagc gccgcgcgcg gcggtgccgg cgcagcctga
     3181 tcgagtagat ttgagagcca tgcttgacca aaaggcgaga aggttcggca ggccgccgat
     3241 aaaggagggc gcgagtggcc atatctgtca gtccggacgt gaggaattcg cccggattct
     3301 gccatatctg ccgccagtga aatcgctcgg cgcaaaaaca aatcatttgc ccaatataca
     3361 ggggctgacc gccgagtcgc ctctacagga tgggcccgga acgaggaata ttggctgaaa
     3421 agacccccca accggccgtc gaagaaatga ggtaatactc atttattcag agattcgtga
     3481 atcggggttt gtatgggcct attcaggggg ttaccgggtc gatcgaatca gcatcgatgt
     3541 ttcaatagcg aaacatcgat gcgtttgatc ggcgtttatt cggccgctgc ggataacgaa
     3601 ggcgtcgcgt tcgccgctga caccatgaaa tcggagatgc gaggcacgat ttccgatttg
     3661 aagcgggatc cgttgaacac accgtagtgt ccgacacctt tttgcacata atgcacccgg
     3721 cgatgatcgg gaatcgcgct gcacagcgcg tgcgtcgcct cggtttgtcc aagaccggag
     3781 atgtcgtcgt tctcgccttc caccgtcatc agcgccacgc gggtgatttt cgacgggtcg
     3841 acgcgcttgc cgcgatgggt catctcgccc ttgggcagag cgtgcttgac gaacacggta
     3901 tcgacggtct gcagataata ctccgccgac agatccatga cggcgagata ttcgtcatag
     3961 aaatcgcggt gcttgtcgac gaggtcgccg tcgcccttca ccagattctt gaacagcgcc
     4021 ttatgggcgt cggtatggcg gtccagattc atgctgatga agcccgagag ctgcaggaag
     4081 cccggataga cgtcgcgcat cacgcccgga tgcggaaacg gcaccttggt gatgacgtgg
     4141 ctgcggaacc agtcgatgcc gcgctcggcg gcgagattgt tgaccgccgt cgggttgcgg
     4201 cgggtatcga tcgggccgcc catcagcgtc atcgagaccg gaaccagagg atcgcgttcg
     4261 gcctccatga tggaaaccgc cgccaccacc ggcaccgaag gctggcacac cgcgatgacg
     4321 tgcatgttgc ggccaagcgt gtgcagcatc tcgatgatgt aatcgacgta gtcgtcgaga
     4381 tcgaagcggc cctcggtcag cggaaccatc cgggcatcgg accaatcggt gatatagacc
     4441 tcatgcgtcg gcagaaatgc ctcgacggtg ccgcgcagca gcgtcgcgta atggccggac
     4501 atcggcgcca cgatcagcac ccgcggctgg gggctgcgca gcgggcgcgt cagcttgcga
     4561 tcgaagtaca gcaggcggca gaacggtttt tcccatacga cgcggatttc aaccggggta
     4621 cggacgccat tgacctcggt gtcgttgagg ccccattccg gtttgccgta acggcgcgtc
     4681 gtccgctcga ataattcgca cccggcggcg acggatttgc cgaactcggt gcgcgaccag
     4741 ggattaagcg gattctgaaa cagcagtttt gtcgcatccg tcacggcgcg cgccggattg
     4801 agcgaggcct gcgccatctc atacatccag tacatcggcg tcgtcagcgc cggactgcct
     4861 tcggccacca gtggcggtgc gccgccaaat tcaccaatcg gcatttaatc ctcaagtctc
     4921 tttttgcatc gcagcatagt gccgaatgcg taataatgcg tcaatgagca gaattggaca
     4981 gataagtata tctacgccgc cgggaagcca aagatcccac aaataaagga taagtgactc
     5041 tattcgccgc tctggatcag cgagccgtgc tgccgggcgc tgcgcaaaag cgcaagaaat
     5101 atcgcaaatg acgcaataag gagcacggcg ttgatcagca gcgcctcgac catgagatcg
     5161 gcccggaatt catggtcgat cagcaacgcc cgcatgccct cgaagacgtg ggtcggcggc
     5221 aacgcccagg cgacatattg cagccagtgc ggcagcaccg ttaccggata gtaaacgcag
     5281 gccagcggca tcaccccgaa catcagcgtc cagacgatac tctccgcccc caggccattg
     5341 cgcagcacca ggcccgagac gaaaataccg agcgaccagc tggtgaaaat caggttacag
     5401 aagaacgcga tcagcggcag cccgatgcca tagaaattga aatcgaagaa aatcatcgcc
     5461 agccccgtca tcggaatgac gccgatcgcg agccggatca ggctcatgac catcagcgcg
     5521 atcaggaact cgatcggctt tagcgggctc atcatcaggt tgccgagatt gcgcgcccac
     5581 atctcctcga gaaacgagat cgaaaagccg agctgcccgc gaaacaggat gtcccacagg
     5641 atgaccgcgc cgatcagcgt gccgccagcg cgggcgaaaa accccgaggt ttgcgagatg
     5701 tagttctgca ggaatcccca ggtgatgatc tgcagcgcgg gccagtagat caattccaaa
     5761 agacgcggcc acgacgacat caaaagatac cagtagcgca ggatcatcgc attgatgcga
     5821 tgcggcgcga tcccccggtg catgacgagt tggctcacga cgcgccctcc tgaatgcggc
     5881 cgcgggcgac gtcgagaaat acttcctcga gcgtggcgcg gttatagcgg gccatgatgc
     5941 cgtcagggct gtcgtcgtcc tcgatgcggc cgcgcttcat gatgatgacg cggtcgcaca
     6001 tccgctccac ttcgagcatg ttgtgcgacg ccagcaggat cgttgcaccc tgtgtcttgc
     6061 gatagcgctc cagatgctgc cggatccagt cggcggtgtc gggatcgagc gatgccgtcg
     6121 gttcgtccag cagcagcagc tcgggctggt tgatcagcgc cttcgccagc gccacccgcg
     6181 tcttctgccc ggcggagagt tttccgttgg ggcggtcgag aaactcgtta agatcaagat
     6241 cggcggcgag tttggcgatc cgctcgcgca aattcgccac cgcatagagc cggccgaaga
     6301 tggtgaggtt ttgccgcacc gtgagccgca tcggcatatc gacatagggg ctttcgaaat
     6361 tcatccggcc cagcacctcg gcgctctgct cgggcatcga acaacccagc acctgaatcc
     6421 gtcccgaggt cggcagcacc agccccatga tcatggcgat ggtcgtggtc ttgccggcgc
     6481 cgttgccgcc gagcagcccg gtgacgctgc cgcgcgcaat gcgaaacgag acgtcgtcga
     6541 cggcgcgggt ggtcttgtag agttttacca ggtgcgcgac ttcgatcgcg gcggatccgg
     6601 ccggatcaat cgcggcaagc ttaggcgggg cggcgtcgtt attcaccatg cggggcgttc
     6661 attgagccat cgcggggtcg agcgcaagac tttggcaata accggtttgg caagaacttg
     6721 ctttggcaag agcttgtttt gacaagaacc tgggcccggc ggcaccgacc tgaatgggtg
     6781 aatttgtggt cgggccgttg cgcggataga ctccggacat gagcgacgtt gccgcctccg
     6841 attttcgcca tccgcgccgc catgtgcggc tggatacgat tttgcggctg cgctggctcg
     6901 cggcgctcgg acagctcgcc gccattttca tcgtgatcca ggggctcgaa ttcgacgtcc
     6961 cggtgatccc ctgcgtgacc atcgtagggt tgtccgccct gctcaacctc gtgctgcaga
     7021 tggccttcaa cccgatgcag cggctggaac cggcctatgc ggcggccctg ctggcgttga
     7081 acatcgtcga actggcggga ttgctgtact tcaccggcgg cctgcagaac ccgttttcgt
     7141 ttctgtttct cgcgccggtc ctgatctctg cgacggcgct gccgatccgg ctgacgatcg
     7201 cgctcggcgc gctcgccatc gcatgcgcct ccgcactggt tttcttccat gagccgctgc
     7261 cgtgggacgg cgatgaacct ctggtgctgc cgccgatcta tttggtcggc gtctggctct
     7321 cgatcgtggt cgcgatcggc gtcaccagcc tttacgcatt ccaggtcacc gaggaggccc
     7381 gcaagctgtc ggacgcgctg gcggccaccg aactggtact gacccgggaa cagcatctca
     7441 cgcagctcga cggtctcgcc gccgccgccg cccacgagct cggaacaccg ctgtcgacga
     7501 tctttctgat ctcgcgcgaa ctggagcggg ccgtgccgga cgccagcccg ctcgcttccg
     7561 acctcaaaac cctgcgcgag caggcgcagc gctgccgcga cattctggcc aagatcactc
     7621 agctgtcctc gaccggtgcg ccgttcgacc tgatgccgtt gtccaccctg atcgaggaag
     7681 tggtggcgcc gcatcgcgac tttggcgtcc agatcaaggt ccggatcgcg gtcgcgggca
     7741 cccgcgaacc ggtcggcacc cgcaatcccg cgatcctcta tggcgtcggc aatatcctgg
     7801 aaaacgccgt cgatttcgcc cgcgcgacgg tggaggtgaa tgcgtggtgg aataccgaga
     7861 ccgtcgaaat cgtcatctcc gatgacgggc cgggcatcgc gcccgacata ctgaagcgga
     7921 tcggcgaacc ctatctgtcg cggcggcgcg gtgccgacga ggcccaaggc gaccaccacg
     7981 gcctcggtct cggggtgttt atcgcccgga cgctgctgga gcgcaccggc gccaaggtct
     8041 ccttcaccaa ccggaccttt ccggatcacg gcgccgtggt tcagatcgcc tggccgcgca
     8101 gccgcttcga ggcgagtgaa acagaagctg aatcaatcgt ttagttccgc gccggatcgc
     8161 tatgacggtt gacctacatt gtgcgggcct tggcagcgcg gcgttgccgg cccatatgtg
     8221 gatgcccgac atgaggatac ccgagcccgt gaccgccatc gccgaactga ttgatcacgc
     8281 cgatcgctcg ctcctgatcg tggaggacga caaggcgttt ctggagcgac tgtcgcgcgc
     8341 catggagacg cgaggcttca cggtcacatc ctgcgacagc gtatccgatg gtttggcaca
     8401 aatcggcaag gcggcaccag ccttcgcggt ggtggatttg cggctcggcg acggcaatgg
     8461 ccttgacgtg gtgtcggcgc tgaagcgcaa gcgccccgac gcgcgcgcga tcgtgctgac
     8521 gggttatggc aacatcgcca ccgccgtgac ggcggtgaag atgggcgcgg tggattatct
     8581 gtcaaaaccc gccgacgccg acgacgtcgt cgcggcattg ctggcgagcg gcaccgagaa
     8641 gtccgagctg ccgatcaatc cgatgtcggc ggaccgcgtg cgctgggagc acatccagcg
     8701 catctacgag atgtgcaacc gcaacgtctc cgaaaccgca cgtcggctga acatgcatcg
     8761 ccgcaccctg cagcgcatcc tcgccaagcg cgcgccgcgg taattgagcg cgcgccgcgg
     8821 taattttctc gacacatcaa gctgccgccg cgaatcgacc ggccgatcgc cggccgtcgc
     8881 gagcccatgc gcgatgaacg ggcagaattg aacctgccgc ttctttgatc acggcagcag
     8941 gtatttttgc ggcttgaacg cgccgggaat taagggctcg ttaggcatag ccagcgataa
     9001 acagacttgt ctgtcgctat catcggcgcg cccggcgcct gcggtccgga cagatcggaa
     9061 ccagccacaa ggggacaagg ccgatggtcg ccgcgattga gcgcatcagc aaacggaagg
     9121 acgcatcgtc gcaacccgag ggcagcgaca aagcgtcaga tatttccgcc ctgatcgcgc
     9181 gattgaccac cgaggtcgac cagatcgcct gcgagaagac caaatcgatc cagcagatca
     9241 ccaaccagat gaaaatgctg gcgctgaacg cgctgatcga aagctcgcgc gccggcgcgc
     9301 tcggcgcggg ctttgccgtg gtggcgcagg aagtccgcag cgtcggccag aaggtggagg
     9361 acatcgcccg cgagttggaa accgagctga ccgggcggac tgccaacctg gcgcagtcga
     9421 tcgaaaagat gatggagcgc tcacgcgggg aacggatggt cgatctcgcg ctcaacgccg
     9481 tcgaactgat cgaccgaaac ctttatgagc gcacctgcga cgtgcgctgg tgggccaccg
     9541 attccgccgt ggtggattgc gccgccagtc cgggtgctgc gaatgtttcc cacgtcgccg
     9601 agcgccttgc cgtgatcctg cgcgcctata ccgtctatct cgatctgtgg ctgtgcgacc
     9661 tcaacggcaa tgtgctggcc agcgcccggt cggaccgcta cgacgtcaag ggggcgagcg
     9721 tcgcccgaat gccctggttt tccgacgcgc tcaagctgcg gtccggcgac gattatgtcg
     9781 ccggcgacat cgagcgtcag ccgctgctcg gcaatgccca gatcgcgacc tattgcgcca
     9841 gcgtgcgcga aggcggcaag gctaacggca agccgatcgg cgtccttgcg atccatttcg
     9901 actgggaagc gcaggcccgc gccatcgtcg aaggcatccg ggtggacgac gcaaaggccc
     9961 gcgtgctgct ggtggattcg aaattccgcg tcatcgcgtc ctccgacggc cagggcctgc
    10021 tgaccgagcg cgtgcccctg aagggcggac gcagcggatt ctacaaggac ggctccggcc
    10081 cgcaggtggc gtttcatctc acccccggct acgagaccta taagggcctc ggatggttcg
    10141 gcgtgatcct ccgttagggc aaggcgcagc gccgaccagg cgtcagcgcc gcacatgcat
    10201 aagcgctctc cccaaaggcc gagttctccc ctcactcgga gcagagccga tttctagaat
    10261 tccccgtggc cctgcggatc gaccaggcgg ttgatccgct gcgccgcggc gatcgcaaac
    10321 cgcaccgtca tcgctttgcg ggtcggtgcc ggcagccgat gctccggtgc ctcgcaatgc
    10381 atatgcgcgc cataggcatc ggccacgatc agcccggttt gcccggggaa aatctcgcag
    10441 ggcagatcct gggtgaaggc aaagaacagc cggtcgcaat gcatccggta gtcctgccat
    10501 ttctgatcgg cgcgcagatc ctcgatcgag gatttgattt cg
//
LOCUS       DATNOI010000139         3727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1711950,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000139 DATNOI010000000
VERSION     DATNOI010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711950"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VN968_05250"
     CDS             complement(<1..726)
                     /locus_tag="VN968_05250"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HXL28679.1"
                     /translation="MTRIALVVSDVDGTLLTKDKTLTDGAKRAVRRLDDAGIRFTITS
                     SRPTVGMRFLIEPLAITLPVGPFNGSSIVDPQLNPIEAHLIPAATAQRSLDVLNEFGV
                     DVWLFTNELWLTRRPDGEYVPHEKLAIRTDPTIVEDFAPYLSTACKIVGASPDAALLV
                     RCEAAMQKAVGAQATVVRSQSYYLDITPPGHDKGTFVQAMAKRQGISTDAVATIGDMQ
                     NDLAMFKKSGVSIAMGNATDDVKK"
     gene            complement(723..1241)
                     /locus_tag="VN968_05255"
     CDS             complement(723..1241)
                     /locus_tag="VN968_05255"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HXL28680.1"
                     /translation="MGVSGSGKSTIADGLAGRLRWTFEDGDRFHPASNVAKMSAGHPL
                     TDEDRWPWLQAIADEIDRVCKAGEHAVIACSALKRAYRDVLVHGRNDARVVFLNGTQE
                     LIASRLARRKGHFMPPGLLASQFSTLEPPLESENPITVSIDASVDAIVDDIVRQLNLT
                     PAERGTPRRNRS"
     gene            complement(1273..2007)
                     /gene="pgl"
                     /locus_tag="VN968_05260"
     CDS             complement(1273..2007)
                     /gene="pgl"
                     /locus_tag="VN968_05260"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HXL28681.1"
                     /translation="MNTKVIEVSDSVALAKAAAERVMARIAANGDRVAICLTGGSSPK
                     QLYQLLATDAFRSRIPWDRVHWFIGDERFVPADDPVNNMGMARQIFLDRRAPASNIHP
                     IPTDTADPDKAARRYENELQRFYGAARLDPARPLFDVVLMGVGPDGHTASLFPDYPAI
                     EETGRWVVGVPQAHVEPFVPRVSLTLPALNSCREMLFEVAGSEKRAILTRVLDGENLP
                     ANRAHSTGETIWLVDTAALPEHFRGR"
     gene            complement(2037..3551)
                     /gene="zwf"
                     /locus_tag="VN968_05265"
     CDS             complement(2037..3551)
                     /gene="zwf"
                     /locus_tag="VN968_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXL28682.1"
                     /translation="MTDVPSNQKKPDPCSFVIFGVTGDLAHRLVIPALYNLAATGLLP
                     DKFCVVGITRKGMPNDELRDGLMKGLRQFATRPVDDAIATRLLECVTCIAADPREPAS
                     FDKMRQQIERLEANRSTGGNRLFYLATPPDAFAPISRELGRTGLLREESGAWRRLVIE
                     KPFGTDLASAKVLNGELLKIVDEHQIYRIDHYLGKETVQNILVLRFANGMFEPIWNRN
                     HIDHVQITVDEKLGVGHRGSFYDSTGALRDMVPNHLFQLLSLVAMEPPIRFDAHSVRS
                     EKADVLAAIQPQSEADALKNSVRGQYRGGRIGDTEIKDYGKTEDVRPGSTTETYAALK
                     LTIDNWRWAGVPFYLRTGKALGIKRTEVAIKFKQAPFAMFSCTAAETLAENYLVIGVE
                     PTEGIALQFNTKVPGPSIRIDGVEMKFRYKDYFKAEPATGYETLIYDCMIGDNILFQR
                     ADSVEAGWKAVQPFLDAWKTAGAKGLQSYKAGSEGPADADELLRRDGRSWRELG"
     gene            complement(3548..>3727)
                     /locus_tag="VN968_05270"
     CDS             complement(3548..>3727)
                     /locus_tag="VN968_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase
                     (decarboxylating)"
                     /protein_id="HXL28683.1"
                     /translation="EVLTAALFARFRSRKQHTFAEKILSAMRDGFGGHKEPQQHPDPV
                     RQNAPDIVKPKADRS"
BASE COUNT          660 a         1138 c         1187 g          742 t
ORIGIN      
        1 tttcttgaca tcgtcggtcg cgttgcccat cgcgatcgag acgccgctct ttttgaacat
       61 ggccagatcg ttctgcatgt cgccgatggt cgcgaccgca tcggtcgaaa tgccctgacg
      121 tttggccatc gcctgcacga aggtgccttt gtcgtgcccc ggcggggtga tgtcgagata
      181 atagctttgc gaccggacca cggtagcctg cgcaccaact gccttctgca tcgcggcttc
      241 gcaacgaacc agcagcgcag catcggggct ggcgccgacg attttgcagg cggtcgaaag
      301 ataaggcgca aaatcctcaa cgatggtcgg atcggtccgg atggcgagct tttcgtgcgg
      361 aacgtattcg ccgtcgggcc ggcgggtgag ccagagttcg ttggtgaaca gccagacgtc
      421 gacgccgaat tcgttcaaga catcgagact gcgttgcgcc gtggccgcgg ggatcagatg
      481 tgcttcgatc gggttcagtt gtggatcgac gatcgaactt ccattgaagg gcccgaccgg
      541 cagcgtgatc gcgagcggct cgatcagaaa ccgcataccg acggtcgggc ggcttgaagt
      601 gatggtgaag cggatgccgg catcatccag ccgccgcacc gcgcgcttgg cgccgtcggt
      661 caaggtcttg tctttggtca gcagcgtgcc gtcgacatcg gagacgacaa gggcaatgcg
      721 ggtcatgagc ggttcctgcg cggcgtgccg cgttcagcgg gagtcagatt cagttggcgg
      781 acgatgtcat cgacgatcgc gtcgaccgaa gcgtcaatcg acacggttat gggattttcg
      841 ctctcgagtg gcggttcgag agtgctgaac tggctcgcca acaggcccgg cggcatgaaa
      901 tggccctttc gccgggcgag gcggcttgca atcaattcct gtgtgccatt gagaaagacc
      961 acgcgagcat cgttgcgtcc atgcaccagg acatcacgat aggcacgctt cagcgccgag
     1021 caggcgatca ccgcatgttc gccggccttg caaaccctgt cgatctcatc ggcgatcgcc
     1081 tgcagccagg gccagcgatc ttcatcggtg aggggatggc cggcgctcat cttggcgacg
     1141 ttgctggcgg gatgaaacct gtcgccatcc tcgaaagtcc agcgtagccg tccggcaagt
     1201 ccgtccgcga tggtgctttt accggagccg gacacgccca tcacgatcaa tgcgcagggg
     1261 gtttcgcctg tctcatcgtc cacgaaaatg ctccggcagc gctgcggtat ccacaagcca
     1321 gatcgtctcg cccgtggaat gcgcgcggtt ggccggcagg ttctcgccat caagcacgcg
     1381 cgtcaagatc gcgcgctttt ccgaacccgc tacctcgaac agcatttcgc ggcaggagtt
     1441 gagcgccggc aaggtcagcg acacccgcgg cacgaatggc tcgacgtgcg cctgcggcac
     1501 gcccaccacc cagcgtccgg tctcttcgat ggcgggataa tcgggaaata gcgatgcggt
     1561 atggccgtcg ggtccgacgc ccatcagcac gacgtcgaac aacggccggg cagggtccaa
     1621 ccgagctgcg ccatagaacc tttgcaactc gttctcgtat cggcgcgcgg ctttgtcggg
     1681 atcagcggta tcggtcggaa tcgggtggat gttggaagcc ggcgcccggc gatccagaaa
     1741 gatctgccgc gccatgccca tgttgttgac cggatcgtcg gccgggacaa aacgctcgtc
     1801 gccgataaac cagtgcacgc ggtcccacgg gatccggctt cgaaaggcat cggtcgcgag
     1861 caattggtag agttgtttcg ggctcgatcc gccggtcagg cagatcgcca cgcgatcgcc
     1921 gttggcggcg atccttgcca tgacgcgttc cgcggcggct ttggcgagcg cgacagagtc
     1981 ggaaacctcg atcactttgg tgttcacttg gcgatgatcg tcccgagcca tccgatctaa
     2041 ccgagctcgc gccagctgcg gccgtcgcgc ctgagaagtt cgtctgcgtc ggcggggcct
     2101 tcgctgccgg ctttgtagct ttgcaggccc ttggcgcccg cggttttcca cgcatcgagg
     2161 aaaggctgca ccgccttcca accggcttcc acgctgtcgg cgcgctgaaa caggatgttg
     2221 tcgccgatca tgcagtcgta gatcagcgtc tcatagccgg tagccggctc cgccttgaag
     2281 taatccttgt agcggaattt catctcaacg ccgtcgatcc ggatcgaggg tccgggcacc
     2341 ttagtgttga actgtagggc gatgccttcc gtcggctcga cgccgatcac aaggtagttc
     2401 tccgccagcg tctccgcggc ggtgcagctg aacatggcga acggcgcctg cttgaacttg
     2461 atcgccactt cggtgcgctt gatcccgagc gccttgccgg tgcgcaggta aaagggaacg
     2521 ccggcccagc gccagttgtc gatggtcagt ttcagcgcgg cataggtttc ggtggtgctg
     2581 ccgggcctga cgtcctcggt cttgccatag tctttaatct cggtgtcgcc gatcctgccg
     2641 ccccgatact ggccgcgcac ggaattcttc agagcgtcgg cctcgctctg gggctggatc
     2701 gccgccagca cgtcggcctt ctcggagcgc accgaatgcg catcgaaccg gatcggcggc
     2761 tccatcgcga ccagcgatag cagctggaac agatggttcg gcaccatgtc gcgcaacgcg
     2821 ccggttgagt cgtagaaact gccgcgatgg ccgacgccga gcttctcgtc gacggtgatc
     2881 tgcacatgat cgatatggtt gcgattccag atcggctcga acatgccgtt ggcaaagcgc
     2941 agcaccagga tgttctgcac cgtttccttg ccgaggtagt gatcgatccg atagatctgg
     3001 tgttcgtcga cgatcttgag cagttcgccg ttcaggactt tcgccgaggc aagatcagtc
     3061 ccaaacggct tttcaatcac cagccgccgc catgcgccgc tttcttccct tagcaaaccg
     3121 gtgcggccga gttccctgct gatcggcgcg aaggcatcgg gcggcgtcgc caggtaaaac
     3181 agacgattgc cgccggttga ccggtttgcc tccagccgct cgatctgctg ccgcatcttg
     3241 tcaaacgagg ccggctccct cggatcggcg gcgatgcagg tcacgcattc cagcaggcgt
     3301 gtggcgatcg catcgtccac cgggcgggtg gcgaattggc gcaggccctt catcaggccg
     3361 tcgcgcagct cgtcgttcgg catgcccttg cgggtgatgc cgaccacgca gaatttgtcg
     3421 ggaagcaggc cggtcgccgc gaggttataa agggccggga tcaccagccg atgcgccagg
     3481 tcgccggtga cgccgaaaat gacgaaggag cacggatcgg gcttcttctg attggaagga
     3541 acgtcggtca cgaacggtcg gcctttggct tgacgatatc gggcgcattc tgccgaaccg
     3601 ggtccggatg ctgttgcggc tccttatggc cgccgaaacc atcgcgcatt gccgagagaa
     3661 tcttttcggc gaaggtgtgt tgcttgcgcg agcgaaaccg cgcgaacaac gccgcggtca
     3721 gaacttc
//
LOCUS       DATNOI010000140         8171 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1158419,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000140 DATNOI010000000
VERSION     DATNOI010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158419"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2070)
                     /gene="purL"
                     /locus_tag="VN968_05275"
     CDS             complement(<1..2070)
                     /gene="purL"
                     /locus_tag="VN968_05275"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HXL28684.1"
                     /translation="MNQPQITPELVASHGLKPDEYERILKLIGRVPSFTELGIFSAMW
                     NEHCSYKSSRLHLRGLPTKAPWVIQGPGENAGVIDIGDGQAVVFKMESHNHPSYIEPY
                     QGATTGVGGILRDVFTMGARPIACLNALSFGAPEHPRTRHLVSGVVAGIGGYGNSFGV
                     PTVGGQVRFHTRYDGNILVNAMAVGLAETDKIFYAAASGVNMPIVYLGSKTGRDGIHG
                     ASMASVEFDDDSAEKRPTVQVGDPFAEKLLLEACLEIMEADCVIAIQDMGAAGLTCSA
                     VEMGAKGDLGVDLDLDAVPTRETGMSAYEMMLSESQERMLMVLKPEKEKEAEAIFRKW
                     GLDFAVVGYTTPSKRFVVKHGGDVMADLPIKELGDEAPLYDRPHVPAPKLPVIHARDV
                     TAPVGILSALEKLIATPELCSKRWVWEQYDHVILGNTVQRPGGDAAVVRVQDGPKGLA
                     LTVDVTPRYCEADPFEGGKQAVAEAWRNITAVGGRPLAITDNLNFGNPERPEIMGQFV
                     GCLKGISEACRALDFPVVSGNVSLYNETNGRGILPTPSIGGVGLLDDFTKSATLAFKD
                     EDEAILLIGETHGWLGQSVYLREICGREEGAPPPVDLAAEKRNGCVVRGMIHASTATA
                     AHDISDGGLLVALVEMAMAGGIGARLEAAPAEIVPHAYWFGEDQARYIVTVPAAEAGL
                     VLAKMKGA"
     gene            2249..2737
                     /locus_tag="VN968_05280"
     CDS             2249..2737
                     /locus_tag="VN968_05280"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXL28685.1"
                     /translation="MDDLTKSAFLNRPDLHVETSGEFKGWRTWSRDNFETLNGPFYHR
                     MDEQGRIHCAFRVENKHLNGMRNVHGGCFMAFADYCLFAMAAPALQGPGVTVSFACEF
                     LDAAREGELVEGTGEIMRAGGSMIFLRGLLKSGERSLFTFSGTLKRVKRGMPPTAGNE
                     GK"
     gene            complement(2746..3720)
                     /locus_tag="VN968_05285"
     CDS             complement(2746..3720)
                     /locus_tag="VN968_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL28686.1"
                     /translation="MRALIRRLSRIAAAWALVVYAGAALAQDYPKRPITMIVPFAAGG
                     TSDVIARTVAEQMGASLGQPIVIENVAGAGGSTALARAARAEPDGYTIAIGNAGTNAA
                     AYTIYPMLPFKPDAFAPVAMVAKTFGIVALRKDFPAKNLQEFIAYAKSNPGKINLGHA
                     GVGSSNFLICKSFVQAAGIEVTLVGYRGAAPALTDAIGGQIDGVCDAAASVSQSIDDK
                     LVRGLVVGSTVRLATLPDLPTSAQAGLPEFEAQGWNGLFAPKGTPPEIVARLNAAARA
                     AVESEPVKKRFADLSTVAPDASEHTPEVLQQLVTRDVEKYRKLLEEKK"
     gene            complement(3717..4418)
                     /gene="purQ"
                     /locus_tag="VN968_05290"
     CDS             complement(3717..4418)
                     /gene="purQ"
                     /locus_tag="VN968_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HXL28687.1"
                     /translation="MKSAVLVFPGINRERDMARALRLASGHEPAMVWHAETSLPKGTD
                     LVVVPGGFSYGDYLRCGAIAARAPIMDAVRSFASEGGLVLGVCNGFQILCESGLLPGV
                     LMRNARLKFICHDVHLRVERSDTPFTRGYNAGQVIRVPVAHGEGNYEADEETLKRLEG
                     DGRVLYRYCSPEGVVDERSNVNGAAQSIAGIVNERGNVLGMMPHPENHVEDIMGCIDG
                     RGLFAGLVAHLERAA"
     gene            complement(4431..4673)
                     /gene="purS"
                     /locus_tag="VN968_05295"
     CDS             complement(4431..4673)
                     /gene="purS"
                     /locus_tag="VN968_05295"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HXL28688.1"
                     /translation="MKARVTVTLKSGILDPQGKAIEGALKSLGVDGIASVRQGKVFDI
                     ELSGSDKAKAEAALKDAADKLLANTVIENYRVEVLS"
     gene            complement(4685..5452)
                     /gene="purC"
                     /locus_tag="VN968_05300"
     CDS             complement(4685..5452)
                     /gene="purC"
                     /locus_tag="VN968_05300"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXL28689.1"
                     /translation="MSRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATAFNAKKHQVIE
                     GKGVLNNRISEYLFQHLNDIGVPTHFIRRLNMREQLIREVEIVPLEVVVRNVAAGSLS
                     QRLGIEEGTQLPRSIIEFYYKNDQLNDPMVSEEHITAFGWATPQEIDDIMALAIRVND
                     FLTGLFLGIGIRLVDFKMECGRLFENEMMRIIVADEISPDSCRLWDIKSNEKLDKDRF
                     RRDLGGLLEAYTEVAKRLGILMENERPAGSGPVLVKS"
     gene            5760..6083
                     /locus_tag="VN968_05305"
     CDS             5760..6083
                     /locus_tag="VN968_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HXL28690.1"
                     /translation="MTTFDKREEGFENKFVHDEELKFKAEARRDRLLGLWVAEKLGLS
                     GEAANDYARTVVAADFQGPGHDEVVRKVMDDLAAKGMAPSESEIRVKMDELMAQAIAQ
                     VKAGT"
     gene            complement(6219..6707)
                     /gene="cynS"
                     /locus_tag="VN968_05310"
     CDS             complement(6219..6707)
                     /gene="cynS"
                     /locus_tag="VN968_05310"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HXL28691.1"
                     /translation="MKRSDLTEKLLDIKRDNGWSWKYICEKIGGYSEVLIVGAILGQM
                     KLTKPQAANAGELFGLSKSEVAMLNETPMRGEGVPMPPSDPLIYRFYELVMVNGPAWK
                     ALIEEEFGDGIMSAIDFDMVMERLPNPKGDRVKITMSGKFLPYKYYGASGNVPEYGSK
                     EG"
     gene            complement(6729..7640)
                     /locus_tag="VN968_05315"
     CDS             complement(6729..7640)
                     /locus_tag="VN968_05315"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28692.1"
                     /translation="MTDKFISIEGIAKRYPGADGGAIAVFEDLWLSMARGEFGCVIGH
                     SGCGKTTVLNILAGLDQPSDGAVIVDRQAIEGTSLDRAVIFQSHALLPWRTVLGNVAY
                     AVTSKWRDWDRARVKAHAQTFIDLVGLTGAEHKRPSELSGGMKQRVGIARALSITPKI
                     MLMDEPFSALDALTRGTLQDEVRRICLETGQTAFMITHDVDEAIYLADKIFLMTNGPG
                     AVLAEIVENPLPKDRGRVDLHRHPYYYAVRNHIVDFLVSRSKTFATDVPDHDPRSVPL
                     VRIGLSEPTIVAAPRTVETSSAQAFAR"
     gene            complement(7793..>8171)
                     /locus_tag="VN968_05320"
     CDS             complement(7793..>8171)
                     /locus_tag="VN968_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL28693.1"
                     /translation="FVIFICSVWPMLLNTAFGVAAVRKEWINVARTLEVGTVRRAFTV
                     ILPAAAPTILTGMRISIGIAWLVIVAAEMLVGGTGIGYFVWNEWNNLSITNVIIAILV
                     IGVVGMLLDQILARFTRMVTFPE"
BASE COUNT         1525 a         2602 c         2499 g         1545 t
ORIGIN      
        1 ggcgcccttc atcttggcca gcaccaggcc ggcctctgcc gccggcaccg tcacgatgta
       61 acgcgcctga tcttcgccga accagtacgc gtgcggcacg atctccgccg gcgccgcctc
      121 cagccgcgcg ccgattccgc cagccattgc catctccacc aacgcaacca agaggccgcc
      181 atcggagata tcgtgcgcag ccgtcgcagt gctggcgtgg atcatgccgc gcaccacgca
      241 gccgttgcgc ttttccgcgg cgagatcgac cggcggcgga gcgccctctt cgcggccgca
      301 gatctcgcgc agataaaccg actgaccgag ccagccatgg gtttcgccga tcaggaggat
      361 cgcctcgtcc tcgtccttga acgcgagcgt cgcggatttg gtgaaatcgt cgagcagccc
      421 gacgccgccg atcgaaggcg tcggcaggat gccgcggcca ttggtttcgt tgtagagcga
      481 gacgttgccg gacacgaccg ggaaatcgag cgcacggcag gcttccgaaa ttcccttcag
      541 gcagccgacg aactggccca tgatctccgg ccgctccgga ttgccgaaat tgagattgtc
      601 cgtgatggcc agcggccgcc cgccgaccgc ggtaatattg cgccaggcct cggccaccgc
      661 ctgtttgcca ccttcgaacg gatccgcctc gcagtaacgt ggcgtgacgt ccaccgtcag
      721 cgctaacccc ttcggcccgt cctgcacccg cacgactgct gcatcgccgc cgggccgctg
      781 cacggtattg cccaaaatga cgtggtcgta ctgctcccac acccagcgtt tggaacaaag
      841 ctccggcgtg gcgatcagct tctccagggc agataagatt ccgaccggcg cggtgacgtc
      901 acgcgcatgg atcaccggca atttcggcgc cggcacatgc ggccggtcgt agagcggcgc
      961 ttcgtccccc aattccttga tcggcaggtc ggccatgacg tcgccgccgt gcttgacgac
     1021 aaaacgcttg ctcggcgtgg tgtagccgac gacggcaaaa tccagccccc atttgcggaa
     1081 gattgcttcg gcctccttct ccttttccgg cttcagcacc atgagcatgc gctcctggct
     1141 ttccgacagc atcatctcgt aggcgctcat gccggtttcg cgcgtcggca ccgcatcgag
     1201 atcaagatcg acgccgagat cgcccttggc gcccatttcg accgccgagc aggtcaggcc
     1261 ggccgcgccc atgtcctgga tcgcgatcac gcaatcggct tccatgattt cgaggcaggc
     1321 ctctagcagc agcttttccg cgaagggatc gccgacctgc acggtcggcc gcttctcggc
     1381 ggaatcgtcg tcgaattcga ccgaggccat cgaggcgccg tggatgccat cgcggccggt
     1441 cttggagccg agatagacga tcggcatgtt gacgccagag gcggctgcat aaaagatctt
     1501 gtcggtttcg gcgagaccga ccgccatcgc gttgacaaga atgttgccgt catagcgggt
     1561 gtggaagcgc acctgcccgc ccaccgtcgg cacgccaaac gaattgccgt agccgccaat
     1621 gcctgcgacg acgccggaca cgagatgccg ggttctgggg tgctccggcg cgccgaaact
     1681 cagcgcattg aggcaggcaa tgggacgtgc gcccatcgtg aaaacgtcgc gcaaaatgcc
     1741 gccgacgccg gtggtcgcac cctgatacgg ctcgatgtag ctcggatggt tgtggctctc
     1801 catcttgaac acgacggcct ggccgtcgcc gatatcgatg acgccggcgt tctcgcccgg
     1861 cccctggatc acccagggcg ctttggtggg caacccgcgc agatgcagcc gcgacgactt
     1921 gtacgagcag tgctcgttcc acatcgccga gaaaatcccg agctcggtga aactcggcac
     1981 ccgcccgatc agcttcaaaa tgcgctcgta ctcatccggc ttcagcccgt ggctggcgac
     2041 gagttcgggg gtgatttgag gctggttcat gaggctcttg gacgatgaag gtctcttatt
     2101 aaggagatcg gatcagccgg aaaaggcctt ttatggcgta atttcccgct gtccaaagct
     2161 ttgcggacgc cctatgcaaa ttcgaggttt gcccgccggg gatggatcgg atttaaggac
     2221 tgaattgctc aaatgaaggc ctgatcgcgt ggacgacctg accaaaagcg cattcctcaa
     2281 ccgccccgat ttgcatgtcg aaacgtctgg cgaattcaag ggctggcgga cctggagccg
     2341 cgacaatttt gaaacgctca acggcccgtt ctaccaccgg atggacgagc aggggcgcat
     2401 ccactgcgcc ttccgggtcg agaacaagca tctcaacggc atgcgcaacg tccatggcgg
     2461 ttgcttcatg gcgttcgccg actattgcct gtttgccatg gccgcgccgg cgctgcaggg
     2521 accgggcgtc accgtgtcat tcgcctgcga atttctcgat gccgcacgcg agggcgaact
     2581 ggtcgagggg acaggtgaaa tcatgcgtgc cggcggctcg atgatctttc tgcgcggctt
     2641 gttgaaatcc ggcgagcgct cgctgttcac attttccggg accctcaaac gggtcaagcg
     2701 gggaatgccg ccaaccgctg gcaacgaggg caagtagcac cgcagctact tcttctcctc
     2761 aagcagcttg cggtattttt cgacgtcgcg cgtcaccagt tgctgaagca cttccggcgt
     2821 atgctcgctg gcatcgggtg caaccgtcga cagatcggcg aaccgtttct tgacgggctc
     2881 gctttccacc gccgctcgcg cggcggcgtt cagcctggcg acgatctcag gcggcgtccc
     2941 cttgggcgcg aacaagccgt tccagccctg cgcctcaaat tcgggaaggc ctgcctgcgc
     3001 tgacgtcggc agatccggca acgtcgccag ccgcaccgtt gacccgacta ccagccccct
     3061 caccagtttg tcgtcgatcg actgcgagac cgaagcggcg gcatcgcaga cgccgtcgat
     3121 ctgcccgccg atcgcgtcgg tcaacgccgg cgccgcgccg cgatagccaa ccagcgtcac
     3181 ttcgatcccc gccgcctgga caaaactctt gcagatcaga aaattcgacg agccgacgcc
     3241 ggcgtggccg aggttgatct tgccgggatt gcttttggcg taggcgatga attcctgcag
     3301 gtttttggcg ggaaaatcct tgcgcagcgc gacgatgccg aaggtctttg ccaccatggc
     3361 tacgggtgcg aacgcgtcgg gcttgaacgg cagcatcgga tagatcgtgt aagcggcggc
     3421 attggtgccg gcattgccga tcgcgatggt gtagccgtca ggctctgccc gcgcggcacg
     3481 cgccagcgcg gtcgagccgc cggcgccggc gacattctcg atcacgatcg gctgcccgag
     3541 gctcgcgccc atctgctcgg cgacggtgcg cgcgatgaca tcggaggtac cgccggccgc
     3601 gaacggcacg atcatggtga tcgggcgttt cggatagtcc tgtgcgagcg cggcgccggc
     3661 ataaacaacg agcgcccagg cggcggcaat tctggatagc cgccgaatta gagccctcac
     3721 gccgcgcgct ccagatgcgc caccagccct gcgaacaggc cgcggccgtc gatgcagccc
     3781 atgatgtctt cgacgtggtt ttccggatgc ggcatcatgc cgagcacgtt gccgcgctcg
     3841 ttgacgatgc ccgcgatcga ctgcgcggcg ccattgacgt tggagcgctc gtcgacgacg
     3901 ccctcaggcg agcagtagcg atagagcacc cgcccgtcgc cctcgagccg cttcagcgtc
     3961 tcttcgtcgg cctcgtaatt gccctcgccg tgggcgacgg gtacgcgaat gacctgcccg
     4021 gcgttgtagc cgcgcgtgaa cggcgtgtcg gatcgttcga cccgcaaatg cacgtcgtgg
     4081 cagatgaatt tcaatcgcgc attgcgcatg agcacgccgg gcaacaggcc tgactcgcag
     4141 agaatctgaa agccgttgca gacgccgagc acgaggccgc cctcggaagc aaaggagcgc
     4201 accgcgtcca tgataggcgc gcgtgccgcg atcgcgccgc agcgcaagta atcgccatag
     4261 gaaaatccgc cgggcaccac cacgagatcg gtgcccttcg gcagcgaggt ctcggcgtgc
     4321 cagaccatcg cgggttcatg acccgacgcc agccgcagcg cgcgcgccat gtcgcgctcg
     4381 cgattgattc cgggaaagac gagaacggcg gatttcatca gcaggagtgc ctagctcaaa
     4441 acctcgaccc ggtaattctc gatcaccgtg tttgccagca gcttgtcggc ggcatccttc
     4501 agcgccgctt cggccttggc cttgtcggag cccgagagtt cgatgtcgaa caccttgccc
     4561 tggcgcacgc tggcgatgcc atcgacgccg agcgacttca gcgcgccttc gatggccttg
     4621 ccctggggat caagaatgcc tgatttcagc gtaacggtga cacgcgcttt cacgggacca
     4681 agcctcagct cttcaccagc accggaccgg aacccgcggg ccgttcgttc tccatcagga
     4741 tgcccagccg ttttgcaacc tccgtgtacg cctccaggag accgccgaga tcgcggcgaa
     4801 aacgatcctt gtcgagcttc tcgttcgact tgatgtccca cagccggcac gaatcggggg
     4861 agatctcgtc ggcgacgatg attcgcatca tctcgttctc gaacaagcgg ccgcattcca
     4921 tcttgaaatc gaccaggcgg atgccgattc ccagaaacag cccggtgagg aagtcgttga
     4981 cgcggatcgc cagcgccatg atgtcgtcga tttcctgcgg cgtcgcccag ccgaacgcgg
     5041 tgatatgctc ctcggagacc atggggtcgt tgagctggtc gttcttgtaa taaaattcga
     5101 tgatcgagcg cggcagctgg gtaccttctt cgatgccgag ccgctgcgac agcgatcccg
     5161 ccgccacatt ccgcaccacc acctccagcg gcacgatctc gacctcgcga atcagttgct
     5221 cgcgcatgtt gagacggcgg atgaaatggg tgggcactcc gatgtcgttg agatgctgaa
     5281 acaggtactc cgaaatccgg ttgttgagga cgcccttgcc ctcgatcacc tgatgtttct
     5341 tggcattgaa cgcggtcgca tcgtccttga agtgctggat cagggttccc ggctccggtc
     5401 cttcatagag aaccttggcc ttgccttcat aaatgcggcg tcgacggctc attgggatgt
     5461 accgtgtgtt gttgaaatcc atgaatttgg tgtgctccgg atgacaaagg attgggaccc
     5521 acgacggagc tgccgtgaag gccaggaacg ggaaacagaa caggaaccgg gcccggaatc
     5581 caacctatcc gattggccta cccagcacaa tcgatccggt ccctcggggc cgagttttat
     5641 ccgcttcgct gcgactctaa gggcgttgtt ttagctactt tctccagtta tctaggcgcg
     5701 cagcaaggct gccgcaacga ggccgccgtc accatttgga cagatcggaa ccttgggcca
     5761 tgaccacatt cgataagcgc gaagagggtt tcgagaacaa gttcgtgcac gacgaggagt
     5821 tgaagttcaa ggccgaggcg cgccgcgaca ggctgctcgg actatgggtg gcggaaaagc
     5881 tcggactgtc gggcgaggcc gccaatgact acgccaggac tgtcgtggca gctgacttcc
     5941 agggccccgg ccacgacgag gtggttcgca aggtgatgga cgatctcgcc gccaagggca
     6001 tggccccgtc cgaatcggag atccgcgtca agatggacga gctgatggcg caggccattg
     6061 cgcaggtgaa agcggggacg tgagggcgag ccctcatcca gaggagcgcg ccttggcgcg
     6121 cgtctcgaag gatgagccag gcatctcatg gttcgagacg cgcgaagccg cgctcctcac
     6181 catgagggcc atttacgtgg ccgtctaagg ggtctctatc acccctcctt ggatccatat
     6241 tcgggcacgt tgccgctggc gccataatat ttgtacggca agaatttgcc cgacatcgtg
     6301 atcttgaccc ggtcgccctt cggattgggc aggcgctcca tcaccatgtc gaaatcaatc
     6361 gccgacatga tgccgtcgcc aaattcctcc tcgatcagcg ccttccaggc gggaccgttg
     6421 accatcacca gttcgtagaa gcgatagatc aagggatcgc tgggcggcat cggcacgccc
     6481 tcgccgcgca ttggcgtctc attgagcatc gccacctcgg atttggacag cccgaacagc
     6541 tcgccggcat tggcggcttg cggttttgtc aatttcatct gcccgaggat ggcgccgacg
     6601 atcaggactt cagagtagcc gccgatcttt tcgcagatgt atttccagct ccagccgttg
     6661 tcgcgcttga tgtcgagcag cttttcggtg aggtcggatc gcttcatgtg tcgtctccct
     6721 gatgaacgct aacgcgcaaa tgcttgtgca gaactggttt cgactgttcg tggcgcagca
     6781 acgatggtcg gctcgctcaa accgatccgc accagcggca cgctgcgcgg atcgtgatcg
     6841 ggcacatcgg tcgcgaaagt tttgctgcga ctgaccagaa aatcgacgat atggttgcgc
     6901 accgcgtagt aatagggatg ccggtgcagg tcgacgcggc cgcgatcctt cggcagcgga
     6961 ttttccacga tttcggccag cacagctccg gggccattgg tcatcaggaa aatcttgtcg
     7021 gcgagataga tcgcctcgtc gacgtcgtga gtgatcatga acgcggtctg cccggtctcc
     7081 agacaaatgc ggcgcacttc atcctgcagc gtgccgcggg tcaacgcgtc gagcgccgag
     7141 aacggctcgt ccatcagcat gatttttggc gtgatcgaaa gcgcacgagc aatgccgaca
     7201 cgctgcttca tgccgccgga gagctctgag ggacgcttgt gctcggcacc ggtcaagccc
     7261 accagatcga tgaaggtttg cgcgtgcgcc ttgaccctgg cgcggtccca atcccgccat
     7321 ttcgatgtta cggcataggc gacgttgccg agcacggtgc gccacggcag caaagcgtgg
     7381 ctctggaaaa tcacggcgcg gtcgaggctg gtgccttcga tcgcttgcct gtcgacgatg
     7441 acggcgccat cgctcggctg gtcgaggcct gccagaatgt tcagcaccgt ggtcttgccg
     7501 cagccggagt gcccgatcac gcagccgaac tcgccgcgcg ccatcgaaag ccagagatct
     7561 tcgaacaccg cgatcgcccc gccatcggcg cccggatagc gttttgcgat cccctcgatg
     7621 gaaatgaatt tgtcagtcat ggcaccaaca tccgtgtccc gcgcgtggtg cgacgcccag
     7681 tggtgcatcg cagatgcggg acccagcttg ctttgggtcc cggttctgca cagcggcact
     7741 gcgcgccgca gtgcgcccgg gacacgagaa taatcgccgt cttcatgccg catcactccg
     7801 ggaacgtgac catgcgcgtg aagcgagcga ggatctgatc gagcaacatg ccgaccacgc
     7861 cgatcaccag aatggcgatg atgacattgg tgatcgacag gttgttccac tcgttccaga
     7921 caaagtagcc aatgccggtg ccgccgacca gcatctcggc ggcgacgatc accagccacg
     7981 cgatgccgat cgaaatgcgc atgcccgtga ggatggtcgg agccgccgct ggcaggatca
     8041 ccgtgaaggc gcgcctgacg gtgccgacct caagcgtgcg cgcgacgttg atccattcct
     8101 tgcggaccgc cgctactccg aacgctgtgt tgagcagcat cggccagacc gagcaaatga
     8161 agatcacgaa g
//
LOCUS       DATNOI010000141         7717 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_995954,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000141 DATNOI010000000
VERSION     DATNOI010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995954"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..846)
                     /locus_tag="VN968_05325"
     CDS             complement(76..846)
                     /locus_tag="VN968_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlbB"
                     /protein_id="HXL28694.1"
                     /translation="MKSFRDIRVLPVVLIAIFSLAVLKVAGLVLDGGYVFDYQPQTNQ
                     KSWAQDTLNFPGSKFDPADITGTTHEKPKEEAAKPPAPVPEVAKPDGVVVFPEPSQPV
                     SASERVILERLQSRRQELEARAREIDIRESLLKAAERRIDSRAEELKAVESRITTATD
                     QKTETDAARFKGIITMYEGMKPKDAAKVFDRLEMSVLFDIASQIAPRKMSDILGLMAP
                     EAAERLTVEMARRAGPDRSASAVELPKIEGKVLPQKPN"
     gene            complement(843..1304)
                     /locus_tag="VN968_05330"
     CDS             complement(843..1304)
                     /locus_tag="VN968_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6468 domain-containing protein"
                     /protein_id="HXL28695.1"
                     /translation="MSHVFGIVIESLVAVLLMVTIGYCMLLNKRLKRLKADEHSLKAT
                     IAELITATEIAERAIGGLKHTVRDVNENLGNQLTSAAQMSQQLKKQLAEGDNIFRRLS
                     RIATAARPAAEPEPEPEPVPERAVPKVSAAKAVAAAAQAFSDRRRADGIAA"
     gene            complement(1301..2503)
                     /gene="fliM"
                     /locus_tag="VN968_05335"
     CDS             complement(1301..2503)
                     /gene="fliM"
                     /locus_tag="VN968_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HXL28696.1"
                     /translation="MAGNSIDDQDAIAAQWEASLDSEDPAEAAAAAAANELSGTMALQ
                     WAAMVEDGSRDFGGGKNGGERVLSQEEIDNLLGFNVGEVNLDDNSGIRAIIDSAMVSY
                     ERLPMLEIVFDRLVRLMTTSLRNFTSDNVEVSLDRITSVRFGDYMNSIPLPAVLCVFK
                     AEEWENFGLATVDSSLIYSMIDVLLGGRRGSTSLRIEGRPYTTIETNLVKRLVEVVMA
                     DAEQAFRPLSPVTFSIDRLETNPRFAAISRPANAAILVRLHIDMEDRGGNIELLLPYA
                     TIEPIRPVLMQMFMGEKFGRDPVWEGHFATEIAQAQISVDAVLYEADIPLKQLMKLKI
                     GDTLPLEMRADALVAVRCGTVTMTEGRMGRVGDRVAIRVTKQLRKPNTTFAMFEKADE
                     QTKLMEAQ"
     gene            complement(2523..3017)
                     /gene="fliL"
                     /locus_tag="VN968_05340"
     CDS             complement(2523..3017)
                     /gene="fliL"
                     /locus_tag="VN968_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HXL28697.1"
                     /translation="MSDTDTADGVAALPASAPKGKLKLIIAAVGLFVIIGGGSVTWFM
                     FFRGHSEEVHAEAAAPPKPAIFIDVPEMLVNLVGAPGERVQYLKAKIVLEVKEEKLVE
                     AIKPSMPRVTDLFQTYLRELRPADLTGSVGLFRLKEELTRRVNTAISPSQVNAVLFKE
                     IVVQ"
     gene            3388..4152
                     /gene="flgF"
                     /locus_tag="VN968_05345"
     CDS             3388..4152
                     /gene="flgF"
                     /locus_tag="VN968_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HXL28698.1"
                     /translation="MENMLLVGLSRQMTLERQMDVVANNVANVNTNGYKADRSLFSEY
                     LNSGAHEDNFKGRDRAVSYVLDNGTFKDFTQGPTEQTKNPLDVAIDGGGFLVVQTQAG
                     ERYTRDGGLQINNQGQLVTAAGDPVLGTSGPIVFQPTDHDINIAADGNVTVVEGIGHT
                     DSVRGKLRLVSFAQAQRLLKEGSNLYAAGEGGAPQPDTKSQVRQGFVEKSNVNSVAEM
                     SRMIEVTRTYTQIATMLQTQSDLHKSAIEKLADVPA"
     gene            4259..5047
                     /gene="flgG"
                     /locus_tag="VN968_05350"
     CDS             4259..5047
                     /gene="flgG"
                     /locus_tag="VN968_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HXL28699.1"
                     /translation="MRALYTAATGMAAQELNVQVISNNIANMRTTGFKKQRAAFQDLI
                     YDHVRRVGAQASDQGTVVPVGVDIGGGVKTVGTPRLMSQGTLAQTGNDLDIAIRGEGF
                     FKIQMPDGSFTYTRDGSFQMDAQGRVVNAQGNPVQPTITIPQNASAIAINAQGQLSVT
                     LPGSTTPTLVGQIGLTRFINKAGLQPIGDNLFTDTPASGPPQDGIANTDGFGDMQQSN
                     LEQANVEVVTEISDLIAAQRAYEMNAKVISAADQMLQSAAALFR"
     gene            5060..6160
                     /gene="flgA"
                     /locus_tag="VN968_05355"
     CDS             5060..6160
                     /gene="flgA"
                     /locus_tag="VN968_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HXL28700.1"
                     /translation="MTLRSLLLAAALLAAASTAASAQVSDDVIAAPVLRASVNVTSDV
                     VRIGDVIDNAGASAQIAIYRAPDLGTTGSLKVAQVLSALQAHRVIGVDTHNLKEISVT
                     RLARTLESKDIELQVARALEHRFGLGDAANLSLTFDRDVQDLRLDASNSGAMQPVAAR
                     FDPRSNRFDVSFEIANDNVATPTRLRFSGSAIETVETAVLARGIERNEVIKFSDVVVE
                     RRPKAEVGGDVASRDRAVGMQARRQLRAGQALKVADIAKPDLVQRDQNVTLTYEATGL
                     YLTIRGKALENGTEGDVVSVLNLQSKRTVSGVVSGRGQVSVSAPAARLPLAADVTSSI
                     PSSELAAPVSVAIHSTSSVAQQVAQHAAPKAE"
     gene            6160..6921
                     /gene="flgH"
                     /locus_tag="VN968_05360"
     CDS             6160..6921
                     /gene="flgH"
                     /locus_tag="VN968_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HXL28701.1"
                     /translation="MVMYTSGFNRLLVNGTLLVFLSVASGCSSIDRLSQIGEKPKLTE
                     IENPTAQPGYKPVQMPMPKPETASYNANSLWRNGSRAFFKDQRAARIGDILTVTVNIT
                     DKANIANETQRSRTSKEDSGITDFVGAKTLGANAQKILPGRLLTEDSTASSDGKGSVN
                     RQEALQTNVAAVVTQLLPNGNLVVEGKQEIRVNFEIRELIVAGIVRPEDIQSDNTIDS
                     SKIAQARIAYGGRGQITDVQQPRYGQQVMDVLLPF"
     gene            7293..7670
                     /locus_tag="VN968_05365"
     CDS             7293..7670
                     /locus_tag="VN968_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28702.1"
                     /translation="MRITTCVIAAAAMFAATAASAQTVNLTGTYRCIQGCRVAPVGSP
                     AFVTQNGWNLNLVNEAGQPSRAWPDATAPMTKIWADDWNVSAIYSPDGMTIQFDNGTI
                     WQRDIGPPPLRVVHVRRQAALIR"
BASE COUNT         1483 a         2485 c         2384 g         1365 t
ORIGIN      
        1 gccctcgccc cgcccattcg aatctagaac tgcgaatcta gaattggcgg tttaaggact
       61 tgttaaatat tcccgctaat tgggcttttg cggcagcacc ttgccctcga ttttgggaag
      121 ctcgaccgcg gaggcggatc ggtccggtcc ggcgcgccgg gccatttcaa ccgtcaggcg
      181 ctcggccgct tccggcgcca tcaggccgag gatatcggac atcttccgag gcgcgatctg
      241 ggaagcgatg tcgaacagca cggacatctc gagccggtcg aacaccttgg cggcgtcctt
      301 cggcttcatg ccctcgtaca tggtgatgat gcccttgaag cgggcggcat cggtttcggt
      361 cttctggtcg gtcgccgtgg tgatccgcga ttcgaccgcc ttcaattcct cggcgcgcga
      421 atcgatccgc ctttcggcgg ccttgagcag gctctcgcga atgtcgatct cgcgggcacg
      481 cgcctcaagc tcctgccgcc gcgactgcag ccgctccagg atcacccgct cggaggcgga
      541 caccggctgc gacggctcgg gaaagaccac gacgccgtcg ggcttggcca cttccgggac
      601 cggggctggc ggtttggcgg cctcctcctt cggcttctca tgggtcgtgc cggtgatatc
      661 agcgggatcg aatttgcttc cgggaaagtt gagggtgtcc tgggcccagg atttctgatt
      721 cgtctgcggc tgatagtcga agacgtagcc gccatcgagc accaacccgg cgaccttcag
      781 cacggcaagg ctgaagatcg cgatcaggac caccggcagc acgcgaatgt cacggaacga
      841 tttcatgcgg cgatgccgtc agcccttctg cggtcggaaa acgcctgggc cgccgcggca
      901 accgccttgg ccgccgatac cttgggtacc gcacgctccg gcacgggctc gggctccggc
      961 tccggctcgg ccgccggcct ggccgcggtc gcgatcctgg acagccgccg aaagatgttg
     1021 tcgccttccg cgagctgctt cttcaactgc tgggacatct gggccgcgga tgtcagctgg
     1081 ttgccgagat tctcgttgac gtcgcgaacg gtgtgcttga ggccgccgat ggcgcgctcg
     1141 gcgatttcgg ttgccgtgat cagttcggcg atggttgcct tcagcgaatg ttcgtccgcc
     1201 ttgagacgct tcagccgctt gttcagcagc atgcaatagc cgatggtgac catgagcaag
     1261 accgcgacca ggctctcgat cacaattccg aatacgtgac tcattgggcc tccatcaact
     1321 tggtttgctc atcggccttc tcgaacatcg cgaaggtggt gttcggcttg cgcagctgct
     1381 tggtgacgcg aatggcgacg cgatcgccga cccggcccat ccgtccttcg gtcatggtca
     1441 cggtgccgca gcgcaccgcg accagggcgt cggcgcgcat ttcaagcggc agggtatcgc
     1501 cgattttcag cttcatcagc tgcttgagcg gaatatcggc ctcgtagagc acggcatcga
     1561 ccgagatctg cgcctgggcg atttcggtcg cgaaatggcc ttcccagacc ggatcgcggc
     1621 cgaatttctc gcccatgaac atctgcatca gcaccgggcg gatcggctcg atggtggcat
     1681 agggcagcag cagctcgata ttgccgccgc ggtcctccat gtcgatatgc agccgcacca
     1741 ggatggcggc gttggcgggc cggctgatgg cggcgaagcg cggattggtt tccagccggt
     1801 cgatcgagaa ggtcaccggc gacagcggcc ggaaggcctg ctcggcatcg gccatcacca
     1861 cttcgaccag ccgcttgacg aggttggttt cgatggtggt gtaggggcgg ccctcgatgc
     1921 gcaacgaggt cgagccgcgg cgaccgccca agagcacgtc gatcatcgaa tagatcaggc
     1981 ttgaatcgac cgtcgcgaga ccgaagttct cccattcctc ggccttgaac acgcagagca
     2041 cggccggcag cgggatcgaa ttcatgtagt cgccgaaccg caccgaggtg atgcgatcca
     2101 gcgaaacctc gacgttgtcg gaggtgaagt tgcgcaagga cgtggtcatc agccgcacca
     2161 gccggtcgaa cacgatttcg agcatcggca gacgctcgta ggacaccatc gccgaatcga
     2221 tgatggcgcg gatgccggaa ttgtcgtcga ggttgacctc gccgacattg aagccgagca
     2281 ggttgtcgat ttcctcctgc gacaggaccc gttcgccgcc gttcttgccg ccgccgaagt
     2341 cgcggctgcc gtcctcgacc atggcggccc attgcagcgc catggtgccg gagagctcgt
     2401 tggccgcagc cgctgccgcc gcctccgccg gatcctcgga atccagcgac gcttcccatt
     2461 gggctgcgat ggcgtcctgg tcgtcgatgc tgttgccggc catggtcttt aaatccgtct
     2521 cgtcactgaa cgacgatttc cttgaacagc acggcgttga cctggcttgg cgagatcgcc
     2581 gtattgaccc gtctggtcag ctcttccttg agacgaaaca ggcccaccga gccggtgaga
     2641 tcggccggcc gcagctcgcg cagataggtc tggaagagat cggtgacgcg cggcatcgaa
     2701 ggcttgatcg cctcgaccag cttttcctct ttgacctcga gcacgatctt ggccttgaga
     2761 tattgcacgc gctcgccggg cgcgccgacc aggttgacca gcatttccgg cacgtcgatg
     2821 aaaatcgccg gcttcggcgg cgccgcggcc tcggcgtgca cctcttcgct gtggcctcga
     2881 aagaacatga accaggtcac agaaccgccg ccgatgatga cgaacaggcc gaccgcggcg
     2941 atgatcagct tgagcttgcc cttgggggcc gaagctggca acgccgcgac gccatctgca
     3001 gtatcggtgt ctgacatcgc ctgcccgtcc ggttggaaaa aacctcgatg cgcgaggcag
     3061 gcttgatgac gcgatacaaa acaaggtccg ccggcgcaca cgcgctctct tggtgcagac
     3121 gctaagtaac aatggttaat ggattctttc gtttggcccg cggctaggaa aaagttgccg
     3181 ggccaacatg gtcaacaagc ccttattgcc gcccccttcg gcgtccgcga aaacatctaa
     3241 cccattgtct aatcgatatt ttctcaactg gcacgggatt cgctgcgtac tcggcgaacc
     3301 gcggcttggg agagcctttt cggttcgctc gatccgcaac cagcctggga gggccgcgcg
     3361 cggatcattt gaaggggaga accaccgatg gagaatatgc ttctcgtcgg actttcgcgg
     3421 caaatgacgc tggaacggca gatggatgtc gtcgccaaca acgtcgcgaa cgtcaacacc
     3481 aacggctaca aggccgacag atcgctgttt tccgagtatc tgaactcggg cgcgcacgaa
     3541 gacaacttca aaggccgtga ccgtgccgtc agctatgtgc tggacaacgg caccttcaag
     3601 gattttacgc aaggcccgac cgagcagacc aagaatccgc tcgacgtcgc gatcgacggc
     3661 ggcggctttc tggtggtgca gacacaagcc ggcgagcgct acacccgcga cggcggcctg
     3721 cagatcaaca accagggcca gctcgtgacc gccgccggcg acccggtgct cggcacctcg
     3781 ggcccgatcg tgttccagcc gaccgaccat gacatcaaca ttgccgccga cggcaatgtc
     3841 accgtcgtcg agggcatcgg ccacaccgac tcggtccgcg gcaagctgcg gctggtcagt
     3901 ttcgcgcagg cgcaaaggct cctgaaggaa ggctccaacc tctacgcggc cggcgaaggc
     3961 ggcgcgccgc agcccgacac caagtcgcag gtgcgccagg gttttgtcga aaagtccaac
     4021 gtcaactcgg tggccgagat gagccgcatg atcgaggtga cgcgcaccta cacccagatc
     4081 gccaccatgc tgcagaccca gagcgatctg cacaaatccg cgatcgaaaa actcgccgac
     4141 gttccggcgt agctgctcat tcgagtgaat gagcagctac gcttcatttt ttgactaagc
     4201 atgatccttt ccgaaaaccg gcagccactt ttcgggatca tgcttcaagg agattgacat
     4261 gcgcgctctt tataccgcgg cgaccggaat ggcggcacag gaactcaacg ttcaggtgat
     4321 ctccaacaac atcgccaaca tgcgcaccac cggcttcaag aagcagcgcg cggcgtttca
     4381 ggacctgatc tatgaccatg tgcgaagggt cggcgcgcag gcgtcggacc agggcacggt
     4441 cgtgccggtc ggcgtcgaca tcggcggcgg cgtcaagacc gtcggtacgc cgcgcctgat
     4501 gagccagggc acgctggcgc aaactggcaa cgatctcgac atcgccatcc gcggcgaagg
     4561 tttcttcaag atccagatgc cggacggctc atttacctac acccgcgacg gctcgttcca
     4621 gatggacgcg caaggccggg tcgtcaacgc gcaaggcaat ccagtgcagc cgaccatcac
     4681 catcccgcaa aatgcctctg ctatcgccat caacgcgcag ggccagctgt cggtgacctt
     4741 gccgggatcg accacgccga ccttggtcgg ccagatcggg ctgacgcggt tcatcaacaa
     4801 ggccggcctg cagccgatcg gcgacaatct gttcaccgac accccggcat cgggcccgcc
     4861 gcaggacggc atcgccaaca ccgacggctt cggcgacatg cagcagagca atctcgaaca
     4921 ggccaatgtc gaggtcgtca ccgagatttc cgacctgatc gccgcgcagc gcgcctacga
     4981 gatgaacgcc aaggtgatca gcgccgccga ccagatgctg caatccgccg ccgcgctatt
     5041 ccgctaaggg agctttgtga tgaccctccg ttctctcctc ctcgccgccg ctttgctcgc
     5101 cgcggcatca accgccgcat ccgcgcaagt cagcgacgac gttatcgccg cacccgtgtt
     5161 gcgcgccagc gtcaacgtca cctccgacgt ggtgcggatc ggcgatgtca tcgacaatgc
     5221 cggcgcctcg gcgcagatcg cgatctaccg cgcgccggat ctcggcacca ccggctcgct
     5281 gaaggtggcg caggtgctca gcgcgttgca ggcgcatcgg gtgatcgggg tcgataccca
     5341 caatctgaag gaaatctcgg taacccggct ggcgcgaacg ctggagagca aggacatcga
     5401 gctgcaggtc gcccgcgcgc tggagcaccg ctttggcctc ggcgatgccg ccaacctctc
     5461 gctgaccttc gaccgcgacg tgcaggatct gcggctggac gcctccaaca gcggcgccat
     5521 gcagccggtc gcggcgcggt tcgatccgcg cagcaaccgg tttgacgtca gcttcgaaat
     5581 cgccaacgac aatgttgcga cgccgacgcg gttgcgtttc agcggcagcg ccatcgagac
     5641 cgtggagacc gcggtgctgg cgcgcggcat cgaacgcaac gaagtgatca aattctccga
     5701 cgtggtggtg gagcggcgcc cgaaggccga ggtcggcggc gacgtcgcga gccgcgatcg
     5761 cgcggtcggc atgcaggccc gaagacaact gcgcgccggc caggccttga aggtcgccga
     5821 tattgcaaag cccgatctgg tgcagcgcga ccagaacgtg acgctgacct acgaggcgac
     5881 cgggctgtat ctgaccattc gcggcaaggc gctggagaac ggcaccgaag gcgacgtcgt
     5941 cagcgtgctc aatctgcaat ccaagcgcac ggtatccggc gtggtatccg gccgcggcca
     6001 ggtgtcggtg tcggcgccgg cggcccggct tccgcttgct gccgatgtca cctcctcgat
     6061 tccgtcgagc gaactggccg ccccggtctc ggtcgccatc cacagcacgt catccgtcgc
     6121 ccaacaagtc gcgcaacacg ccgctccaaa agcagagtaa tggtaatgta tacgtccggg
     6181 ttcaaccgcc tccttgtcaa cggcacgctc ttggtcttcc tcagcgtcgc gagcggatgc
     6241 tcgtcgatcg atcggctgtc gcagatcggc gaaaagccaa aactgaccga gatcgaaaat
     6301 ccaacggcgc agcccggcta caagccggtg cagatgccga tgccgaaacc ggagaccgcg
     6361 tcctacaacg ccaattcgct gtggcggaac ggctcccggg cgttcttcaa ggatcagcgc
     6421 gcggcgcgga tcggcgacat cttgacggtg acggtgaata tcaccgacaa ggccaacatc
     6481 gccaacgaga cccagcgcag ccgcaccagc aaggaagatt cagggatcac cgatttcgtc
     6541 ggcgccaaga cgctgggcgc gaacgcgcaa aagatattgc cgggccggct attgacagag
     6601 gactccaccg cctcgagcga cggcaagggt tcggtcaacc gccaggaggc gctgcagaca
     6661 aacgtcgccg cggtggtgac gcagctattg ccgaacggca atctcgtggt cgagggcaag
     6721 caggagatcc gggtcaattt cgaaatccgc gaactgatcg tcgccggcat cgtgcgcccg
     6781 gaggacatcc agagcgacaa caccatcgat tcctcgaaga tcgcgcaggc ccgcatcgcc
     6841 tatggcggcc gcggccagat caccgacgtg caacagccgc gctacggcca acaggtgatg
     6901 gacgtgctgc tgccgttcta gttttgcccg ggcatgatcc cgaaaagtgg ataccggttt
     6961 tcggacgaga tcatgcccac ttaatagaga gctcccacac ttcgccgcga accctgggcg
     7021 cggtgaagtg ttccaacgcg gcctccgtca gctcccctgg cggaggccgc ggttggttgt
     7081 ggcgttcacc ccgaggatcg ctggcattcc cgcgcgattg aaaacccaaa atcccggcaa
     7141 cgggatcgcc gggaaagttc cgtcacgggt ggcgccgaac ggaaccggct tgccgccgtt
     7201 tgtgatggaa ccaaacgcgc gtgatccgaa taatcaagga tccctccgga agcccaatgg
     7261 tgggatcagc gtaatccgat ggagtgatct tcatgagaat aacaacatgc gtcatcgcgg
     7321 ccgcggccat gttcgctgca accgcagcat cggcacagac ggtcaatctg accggcacgt
     7381 atcgatgcat ccaagggtgt cgggtggctc cggtcggcag ccccgccttc gtcacgcaga
     7441 acggatggaa tctaaatctc gtcaacgaag ccggccagcc ttcccgtgcc tggcccgatg
     7501 cgacggcgcc gatgaccaag atctgggccg atgactggaa cgtgagcgcc atctattcgc
     7561 ctgacggcat gacgatccag ttcgataacg gaacgatctg gcagcgcgac atcgggccgc
     7621 ccccgctccg ggtggttcac gtgcgaagac aagccgcgct catcaggtga tgcgcaggcc
     7681 gcatcgccta tgggcggccg cggccagcac gcagcac
//
LOCUS       DATNOI010000142         2065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_811145,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000142 DATNOI010000000
VERSION     DATNOI010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811145"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VN968_05370"
     CDS             complement(<1..297)
                     /locus_tag="VN968_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HXL28703.1"
                     /translation="MFMKFLFALFVFLLPALAEAADVTGVPKIIEGDQIQIGNSRFRL
                     GGIDAPSVDQLCLNHTGERWTCGVAARDELIKHADNKTWTCHTRQVVDRRGRTVA"
     gene            complement(571..>2065)
                     /locus_tag="VN968_05375"
     CDS             complement(571..>2065)
                     /locus_tag="VN968_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28704.1"
                     /translation="ARDDEAPDWTAIGQPVAALLDTIDLEHPRPGPARAPARAPDLAE
                     IESIIRTCGGHLLRSFGQNGFIPTYAAFNLMGDPDVGGREVLMALNGLDARGYKNSTL
                     LFNLARVFIARSPARALIRPAWTGIAEPMWQPMQIRHRSAYYDAFFTEALLSFVDTGL
                     ASRTETAASRRAIAEMVDFCLTTSRENVRGQDGRIVSVVTALAPLPHPRFSRFFSQIK
                     QDLGFGIYVPDCDTTACAFSAATQAGSNDPILAQPLLDFYAGYQVGGGANEPKVTVPL
                     NDNIDYEGGVVTWIDNLAGERPFGNDLDPTLNLDILEVSFRNLARWKIIETPQRLDTV
                     RRIIGFQRRLVASGAFTNPRSHIYYLPELYCAYFGRCYAAFFALPPAAQKAIDPDGAH
                     EFIRAKVLVYVQTELIAGEMNAFDAALALIALCHLGADAKTFIPALYCIVSSLGEGGR
                     HGPFKAYEWNKMKTPTRILVGGPEATSAFVLMALALARRVMMREAGL"
BASE COUNT          401 a          621 c          685 g          358 t
ORIGIN      
        1 cgccacggtt cggccgcgcc ggtcgacgac ctgacgggtg tgacaggtcc aggtcttgtt
       61 gtcggcgtgc ttgatcagtt cgtcgcgcgc ggcgacgccg caggtccagc gctcgccggt
      121 atggttgagg caaagctggt cgaccgaggg cgcatcgatc ccgcccagac ggaagcggct
      181 attgccgatc tggatctgat cgccctcgat aatttttgga acgccggtga cgtcggccgc
      241 ctcggccagc gccggcagca gaaatacgaa aagcgcgaac aggaatttca tgaacatcca
      301 aaacggccgg attcaaactg ggctgatatt aacatgcggg cggattgtgg tggggattcg
      361 gttggcacac cagcgcccgg ccgggcggtc gctttcaact taatgcgatg acaagctata
      421 caaagggtcg tccctgatcg cagggaccca tacggcgcgg agtgtcgttt cggcacagca
      481 gcagacacct ttttcacttt tgaggccagg ggtgacgggt ccgtgcgttc gcagggacga
      541 ctcgctggga gtttgtgcca agctaccctg ctacaaaccc gcttcccgca tcatcacccg
      601 gcgcgccagc gcaagagcca tcagcacgaa cgccgaggtg gcctcggggc cgccgaccag
      661 gatgcgggtc ggggttttca tcttgttcca ctcataggcc ttgaacggtc catggcggcc
      721 gccttcgccg agactgctga cgatgcaata cagggccggg atgaaggttt ttgcatccgc
      781 gccgagatgg caaagcgcga tcagcgccag cgccgcatcg aacgcgttca tttcgcccgc
      841 gatcagttca gtctgcacgt aaacgagcac cttggcccgg atgaattcgt gcgcgccatc
      901 ggggtcgatc gccttctgcg ccgcaggggg aagtgcgaag aaggcggcat agcagcggcc
      961 gaaataggcg caataaagct ccggcaggta atagatgtgc gagcgcggat tggtgaaggc
     1021 gccgctcgcg accagccgtc tttgaaagcc gataatgcgg cgaacggtgt ccagccgctg
     1081 cggggtctcg atgatcttcc atcgcgccaa attgcgaaaa ctgacttcga ggatgtcgag
     1141 attgagtgtc ggatcgagat cgttgccgaa cggccgttcg ccggcgaggt tgtcgatcca
     1201 ggtgacgacg ccgccttcat aatcgatgtt gtcattgagc ggcacggtca ccttgggctc
     1261 gttggcgccg ccgccgacct ggtagccggc atagaaatcg agcaacggct gcgcgaggat
     1321 cggatcattg gagccggcct gcgtcgccgc cgagaaggcg caagccgtgg tatcgcaatc
     1381 cggaacgtag atcccaaaac cgagatcctg cttgatctgg gagaagaacc gggaaaagcg
     1441 cggatgcggc agcggcgcca gcgcggtgac gacgctgacg atacggccgt cctgcccgcg
     1501 cacattctcc cggctggtgg tgagacagaa atcgaccatc tcggcgatgg cgcggcgcga
     1561 cgccgcggtc tcggtccgtg aggcgagccc ggtgtcgaca aagctcaaga gcgcctcggt
     1621 gaaaaacgcg tcgtaatagg cggagcgatg gcggatctgc atcggctgcc acatcggttc
     1681 ggcgatgccg gtccaggccg gcctgatgag ggcgcgggcg ggcgagcgcg caatgaagac
     1741 ccgcgccaga ttgaacagca gcgtcgaatt cttgtagccg cgtgcatcga gaccattcag
     1801 cgccatcagc acctcgcggc cgccgacatc cgggtcgccc atcaggttga aggcagcata
     1861 ggtcggaatg aaaccgttct ggccgaacga tcgcaacaga tgtccgccgc aggtgcggat
     1921 gatgctttcg atctcagcca gatcaggcgc gcgcgccggc gcccgcgcag gcccgggcct
     1981 ggggtgctca aggtcgatcg tgtccaaaag cgccgcgacc ggctgcccga tcgccgtcca
     2041 gtccggcgcc tcatcgtcgc gcgcc
//
LOCUS       DATNOI010000143         5537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2084008,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000143 DATNOI010000000
VERSION     DATNOI010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2084008"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VN968_05380"
     CDS             complement(<1..414)
                     /locus_tag="VN968_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXL28705.1"
                     /translation="MMLKAVIWDFGGVLTTSPFEAFARFETERGLPADIIRRTNASNH
                     WENAWAKFERAEVDLETFDQLFAAESLALGAEVRGKDVLPLLSGDLRPEMVEALRRIG
                     TELKTGCITNNLPANAIGSLGGRSLYVAEVMALFDH"
     gene            546..1307
                     /locus_tag="VN968_05385"
     CDS             546..1307
                     /locus_tag="VN968_05385"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HXL28706.1"
                     /translation="MKIMLQVIAGLALLVSAPAHAIVGGGAPSAEGVGRSIVTIVGSR
                     GNFCTGALIAPRLVLTAAHCVQPGATYKIVEYGADRQPQLQDVKTVAIHPGFNMQAMT
                     AHRATADVALLQLEVAPKGKNPATLGVPQVPIVVGSRFTIAGIGVTIRGEGSSGGTIR
                     VAGLVAAGRPGSLQIRLVDPAGQGARDGLGACTGDSGGPVFEDRQDGSIIVGVISWST
                     GPNGTAGCGGLTGVTPLTLYRDWIVQTARQWGSAL"
     gene            complement(1419..2261)
                     /locus_tag="VN968_05390"
     CDS             complement(1419..2261)
                     /locus_tag="VN968_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXL28707.1"
                     /translation="MKARASNLMIGSMTLAVIAAAFAGLLGYRKIHGIAQQAPLRIVF
                     EGSASGLRKGGNVNFDGVQVGEIKSLKLDNPRKVVALTMVDNSAPIRKDTVVGLEFLG
                     LTGVAAISLTGGAAAAAPVPLDVDGIPTLTADLSEIESIRDTLHNVDRILFDNRAMLR
                     DALLSFETYTASLASRGDAIDSIMGKADGAFENFDSAMTKIDSVVPGLAHGSAEELYD
                     KVRTIRELADSFNKRSGAFMEEGRRSLLDISQAAIKVTRKLDPQAVSGDNPPAPRPPR
                     QKRQ"
     gene            complement(2374..3081)
                     /locus_tag="VN968_05395"
     CDS             complement(2374..3081)
                     /locus_tag="VN968_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28708.1"
                     /translation="MSAATHPSWLAGIMAVSPEALGLVMLEADLHARRLDGATQLAAV
                     RDALADGAATAETLRARFPDLAPHEIAGRLDVPVAETDDDPLVGRLWRFAEYRGRPAR
                     ILLYSRGLAPLDGVIAGGLAARLLGLATPREVFIAHELFHHIEATRSDTPIARRYQPT
                     LFRIGGWHWRTGIAALAEIAAGAFAQSLLDLPCHPGVLDLLALEAVAPNATAAGIAAR
                     IAATDRMNGDAGRKLTP"
     gene            complement(3086..5257)
                     /locus_tag="VN968_05400"
     CDS             complement(3086..5257)
                     /locus_tag="VN968_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HXL28709.1"
                     /translation="MTQTSPPARAYRIGIDVGGTFTKAVLIDNATHEVVGRFSVLTTH
                     ADPRGVAKGVVEVFRNVLEQSGVDPKQVIFLAHSTTQATNALLEGDVASVGVIGMATR
                     MESLLAKGQSAMKPIELAPGRHLKPVNRFISKEALTDETARKAIEELKAEGAKVIVAS
                     SAFGVDDSDAEETIRRLVVDAGLAATCGHEITKLYGLTTRTRTAVINASILPKMIDTA
                     NMTEASVREAGIQAPLMIMRGDGGVMDIREMRRRPAMTMLSGPAASVAGALMHLRVSD
                     GIYFEVGGTSTNIGVIRNGRPTVKYARVGGHETYVSSLDVRVIGIAGGSMVRVRDHRL
                     VDVGPRSAHIAGLPYAAFANPEDIVEPRIELFQPKPDDPDDYVAVRVANGTRYAITNT
                     CAANVLGYAKPGLHAYGSPEAAQRAMAALADWVGKSIADTARIILDIATDKVIPVVDD
                     LIREYKLDRDQAVLVGEGGGAAALIPHASSRTGLHHVISKDAEVISSIGVALALVRDV
                     VERVIPNPTPEDLKRIRREAFEAVVKLGAAPENVEVTIEVDQHTHRVRATAMGASEMR
                     ARQRSDEIGEVEAREIAAKAMGVGLERVTLAATTPRMRVFQGEVEERSWRIFTKRRSP
                     VRAVDLDGVIRVQRSHSVVRSASARTGFADVRRLWEDTTIYNGDSVITPDMFVIIGSQ
                     IIDLSGVNSIDQALAITRSEFDGLTASEPVALISIPPTRGL"
     gene            complement(5268..>5537)
                     /locus_tag="VN968_05405"
     CDS             complement(5268..>5537)
                     /locus_tag="VN968_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28710.1"
                     /translation="GPMVVIGTGAALLAIILANKDIPIPYLYSIWLAPTVLQGSMDPT
                     NSWTLWTIGYTKVSHGQFLKTALPFGCMMVAVNSLICYLMLGSLN"
BASE COUNT          952 a         1770 c         1783 g         1032 t
ORIGIN      
        1 atgatcgaac agcgccatca cctccgcgac gtagagcgat cggccgccca ggcttccgat
       61 ggcgttggcg ggaaggttat tggtgatgca cccggtcttg agctcggtcc caatccgcct
      121 gagtgcctcg accatctcgg gtcgcagatc tcccgacagc agcggcaaga cgtctttgcc
      181 tcgaacctcc gcgccgaggg cgagcgattc ggccgcgaac agttgatcga aggtttccag
      241 atccacttcg gcgcgctcga acttcgccca ggcattttcc caatgattgc tcgcattggt
      301 gcgcctgata atatcagcgg gtaacccgcg ttcggtctca aagcgcgcga acgcctcgaa
      361 gggcgacgtg gtgagcacgc cgccaaaatc ccagatcact gccttaagca tcattccctg
      421 ccgtgtgaac gccgattggg gaaacggcta acacggcgcc ttcgcgggga ccagcctgga
      481 tcgccgctcg atcgcccctc gatcgcccct tggcgatgcc tgccgtgcgg gctattgctg
      541 tcgaaatgaa aatcatgctc caggtcattg cgggtttggc gttgctggtc tccgcgcccg
      601 ctcacgcgat cgtcggcggt ggggcgccgt ccgccgaggg cgtcggccgc tccatcgtca
      661 ccattgtcgg ctcgcgcggt aatttctgta ccggcgcctt gatcgcaccg agactggtgt
      721 tgacggcggc gcattgcgtg cagccaggag cgacctacaa gatcgtcgaa tatggcgccg
      781 accggcagcc tcagctgcaa gacgtgaaaa ccgtggccat tcacccggga ttcaatatgc
      841 aggcgatgac ggcgcatcgc gccaccgccg acgtggcgct gctgcagctc gaagtcgcgc
      901 cgaagggaaa aaatccggcg acgctcggcg tgcctcaggt tccgattgtg gtcggcagcc
      961 ggttcaccat agccggcatt ggcgtgacga tccgcggtga aggcagcagc gggggcacca
     1021 ttcgcgtcgc cggcctggtc gcagccggcc ggccaggcag cttgcagatt cgcctcgtcg
     1081 atccggctgg acagggtgct cgcgacggac ttggcgcgtg taccggggat tccggtggcc
     1141 ccgtattcga ggatcggcag gacggctcca tcatcgtcgg cgttatcagc tggtcgaccg
     1201 gaccaaacgg caccgcgggc tgcggcggcc tgaccggcgt cacgccgctg acgctttacc
     1261 gcgactggat cgtgcagacc gcccgccagt ggggttcggc gctgtagagc gttttcaagc
     1321 gaagtggaga ccggttcgcg tcaagaaaac gcgtcaaatc aagaatctag agccccgttt
     1381 cgattcatcg aaacgaaata gggctttagc gcggaggctt actgtcgctt ttgccgcggg
     1441 ggccttggcg ccggcgggtt atctccgctg acggcttgcg ggtcgagttt gcgagtcact
     1501 ttgatagctg cctggctgat gtcgagcagg gagcgacggc cctcctccat gaaggccccc
     1561 gaacgcttat tgaagctgtc ggccagttcg cgaatggtcc gcactttatc atacaattcc
     1621 tcggcgcttc cgtgcgccag gcccggcacg acgctatcga tctttgtcat ggcgctgtcg
     1681 aagttctcga acgcaccgtc ggcttttccc atgatgctat cgatcgcgtc gcccctgctc
     1741 gccaaagacg cggtataggt ctcgaaagaa agcagcgcgt ctctcagcat cgcccggttg
     1801 tcgaacagga tccggtcgac gttgtgcagt gtgtcgcgaa tcgattcgat ctcactgagg
     1861 tcggcggtca gggtcggaat gccgtccaca tccagcggta ccggagctgc tgccgccgcg
     1921 ccgccggtca gcgatatcgc cgccacgccc gtgagtccca ggaactccag gcccacgacc
     1981 gtgtccttgc ggatcggtgc gctgttgtcc accatggtga gggcgaccac cttgcgcggg
     2041 ttgtcgagct tgagggattt tatttctcca acctgaacgc catcgaagtt cacgttgccg
     2101 cctttgcgca gaccgctggc ggagccttcg aaaacgatgc gaagcggagc ttgctgcgcg
     2161 atgccatgga ttttccggta tccaagcaag ccggcaaacg ccgcggcgat gaccgcgagc
     2221 gtcatggatc cgatcatgag attgctcgcc cgcgctttca tcccggaatc cggctgcgat
     2281 gtgcccggtt gatagatcta gtacatctcg ccatcgctcg attgtaccgg caaagctgac
     2341 gatgcgaaag ctcgatgcta caggccggtg gcatcaaggc gtgagcttgc ggccagcgtc
     2401 gccgttcatc cggtccgttg cggcgatgcg ggcagcgatc ccggcggcgg tggcgttcgg
     2461 ggcgacggcc tcgagcgcga ggagatccag caccccgggg tgacagggca gatcgagaag
     2521 cgactgcgca aaggcaccgg ccgcgatctc ggcgagcgcc gcaatgccgg tccgccaatg
     2581 ccagccgcca attcggaaca aggtcggctg gtagcgccgt gcgatcggcg tgtcgcttcg
     2641 ggtcgcctcg atgtgatgga acagctcatg ggctatgaaa acctctcgcg gcgttgccag
     2701 cccgagcaac cgcgcggcca agccgcctgc gatcacacca tcgagcggcg cgagcccgcg
     2761 gctgtagagc agaatgcggg ccggccgccc ccgatattcg gcaaaccgcc agagccggcc
     2821 gaccagcgga tcgtcatcgg tctctgccac cggcacgtca agccggccgg caatttcatg
     2881 cggcgcgaga tcggggaaac gcgcccgcag ggtttccgct gtcgcggctc cgtccgcgag
     2941 cgcatcgcga accgcggcga gttgggtggc gccatcgagc cgccgggcgt gaaggtcggc
     3001 ttccagcatg acaagcccga gcgcctcagg tgacaccgcc atgatgccgg ccagccagga
     3061 cgggtgtgtc gccgccgaca ccacgttaca gcccgcgcgt cggcggaatg ctgatcagcg
     3121 ccaccggttc gctcgcggtg agcccgtcga actcgctgcg ggtaatggcc agcgcctggt
     3181 cgatcgagtt gacaccggac agatcgataa tctggctgcc gatgatcacg aacatatccg
     3241 gcgtgatgac gctgtcaccg ttgtagatcg tggtatcttc ccacagccgg cggacgtcgg
     3301 cgaagccggt acgcgcggaa gccgagcgca ccacgctatg gctgcgctgc acgcggatga
     3361 cgccgtcaag atcgaccgcc cgaaccgggc tgcggcgttt ggtgaatatc cgccacgacc
     3421 gctcctccac ctcgccctgg aacacgcgca tccgcggcgt ggtggcggcg agcgtcacgc
     3481 gctccaagcc gacgcccatc gccttggctg caatttcgcg cgcctcgacc tcgccgatct
     3541 cgtcgctgcg ctggcgggcg cgcatctcgg acgcacccat cgcggttgcg cgaacgcggt
     3601 gggtatgctg atcgacctcg atcgttacct cgacattctc gggcgcggcc ccgagcttga
     3661 ccacggcctc gaatgcctcg cggcggatcc gcttcagatc ctcaggcgtc ggattgggga
     3721 tcacgcgctc taccacgtcg cgcaccaggg cgagcgcgac gccgatcgag gaaatcacct
     3781 cggcgtcctt tgaaatcaca tggtgaaggc cggtgcggct tgaagcatgg ggaatcagcg
     3841 cggccgctcc gccgccttcg ccgacgagca cggcttgatc gcgatcgagc ttgtattcgc
     3901 ggatcagatc atcgaccacc gggatgacct tgtcggtggc gatatcaaga atgatccgtg
     3961 cggtgtccgc gatggatttg ccgacccaat ccgccagcgc cgccatggcg cgctgcgcag
     4021 cctcggggct gccgtaggca tgcagccccg gtttggcgta gcccaggaca ttggcggcgc
     4081 aggtgttggt gatggcataa cgggtgccgt tggcgacgcg taccgcgacg tagtcgtcgg
     4141 gatcatcggg cttcggctga aacagttcga tccggggttc gacgatatct tcagggttgg
     4201 cgaaggcggc gtagggcagg ccggcgatat gagcgctgcg cggcccgacg tcgacaagtc
     4261 tgtgatctct gacccggacc atgctgccgc cggcgatacc gatcacgcgc acgtcgagcg
     4321 aactgacgta ggtctcgtgg ccgccgaccc gcgcatattt gaccgtggga cgtccattcc
     4381 ggatcacccc gatattggtg ctggtgccgc cgacttcaaa gtaaatgccg tccgacaccc
     4441 gcaaatgcat cagcgcgcct gcgacgcttg cggcgggacc cgagagcatc gtcatcgccg
     4501 ggcgacggcg catctcgcgg atgtccatca cgccgccgtc gccacgcatg atcatcaggg
     4561 gcgcctggat cccggcttcg cggacgctgg cctcggtcat gttggcggta tcgatcattt
     4621 tcggcaggat actggcgttg atcaccgcgg tgcgggtcct ggtggtcagg ccatacagct
     4681 tggtgatctc gtgaccgcag gtggcggcta gcccggcgtc caccacgagc cggcgtattg
     4741 tttcctcggc gtcgctgtcg tcgacgccga aggctgaact cgcgacgatg actttggcgc
     4801 cctcggcttt aagctcttcg atcgccttgc gagcggtctc gtcggtcagc gcttccttgc
     4861 tgataaaccg gttgaccggc ttcagatggc gtccgggcgc gagctcgatc ggcttcatcg
     4921 cgctctggcc cttggcaagc agcgattcca tgcgggtcgc catgccgatc acgccgacgc
     4981 ttgccacgtc gccttcgagc aatgcattgg tcgcctgcgt ggtggaatgg gcgagaaaaa
     5041 tcacctgctt cggatccacg cccgactgct caagaacgtt gcgaaaaacc tccaccacgc
     5101 cttttgcgac cccgcgcggg tcggcgtgcg tggtcagcac cgagaagcgg cctaccacct
     5161 cgtgggtcgc gttgtcgatg agcacggcct tggtgaaggt cccgccgaca tcgatgccga
     5221 tgcggtaggc gcgggccggc ggtgaggttt gggtcaacgt ctcgtcctta attgagcgag
     5281 cccagcatca ggtagcagat caacgagttg actgcgacca tcatgcagcc gaagggcagc
     5341 gccgttttga ggaattgccc atggctgacc ttggtgtacc cgatcgtcca caatgtccat
     5401 gaattggtcg ggtccatgct cccttgcagc accgtcggcg ccagccagat cgagtagaga
     5461 tacggaatcg ggatgtcctt gttggcaaga atgatggcaa gcaatgccgc gcccgttccg
     5521 atcaccacca tcggccc
//
LOCUS       DATNOI010000144         9224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2246162,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000144 DATNOI010000000
VERSION     DATNOI010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9224
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2246162"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..796
                     /gene="ureE"
                     /locus_tag="VN968_05410"
     CDS             254..796
                     /gene="ureE"
                     /locus_tag="VN968_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HXL28711.1"
                     /translation="MLRIDRVLGSRLEAAFSEAIHRLEHRGAVDVVNIPVVDLDRRRL
                     LATTRAGEELAIALPRHQKLFDGAVLLIDDARAIVVRAATERWMRLEPRSISDAIELG
                     YHAGNLHWRVRFQGEALFVALEGRPEDYTARLGEMIAARRVGVSILDEEVRDGGGEKG
                     TPKGDGEAATKDHFHHDGHR"
     gene            793..1467
                     /locus_tag="VN968_05415"
     CDS             793..1467
                     /locus_tag="VN968_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HXL28712.1"
                     /translation="MMLSILQAIWQADGTFPSGSFAFSYGVEGVVAQHPRLDGTALAE
                     LTTTIIRQRWATCDRIALVQAFHAAGDLRAIATIDRDVEASTFGSTMRDGSRRNGGSF
                     LASHARLGDATALRLRDAVRAGICLGHIAVMQGAVWQAMGLDEKLAQLASAYGVASGV
                     TAAAVRLGAIGALGGQKVLRGCLPLIETLVSQPVPEDAELSSFVPFLEIAAARHARAD
                     LRLFVN"
     gene            1481..2176
                     /locus_tag="VN968_05420"
     CDS             1481..2176
                     /locus_tag="VN968_05420"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HXL28713.1"
                     /translation="MLLTPTELERLTIFTAAELARRRRAKGLKLNYPEAVAIITDEIL
                     EGAREGRSVADLIGHGSTILSTDDVLSGIAAMMNIIQIECVFPDGTKLVTVHEPIRPA
                     PGSQADPEHPGLVTAADGSIDLNAGRRSLKLKARNTGDRPIQIGSHFHFFEVNKAMEF
                     DRAAAFGMRLDIPAGTAVRFEPGAWKEVSLVTFGGRSELTGLNNLTNGLSSDPAVREG
                     ALEQAKKRGFKGS"
     gene            2178..3887
                     /gene="ureC"
                     /locus_tag="VN968_05425"
     CDS             2178..3887
                     /gene="ureC"
                     /locus_tag="VN968_05425"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HXL28714.1"
                     /translation="MASIDRRDYAALYGPTTGDAVRLGDTALTAVVEKDHAVYGDECL
                     HGGGKTLRDGIGMAGITSAEGALDFLLCNVVVIDPVVGIVKGDLGIRHGRIVGIGKAG
                     NPAIMDGVDPRLIVSSGTTVRDCEGLIATPGAIDVHVHFDSAGLVEHALASGITTMIG
                     GSLGPITVGIDSGGPFNTGKMLQAAEAWPMNFGFLGRGNSHRVPPLVEQLETGVMGLK
                     LHEDWGSMPAAIDSCLKVADEHDFQVQIHTDTLNESGFVEDTLEAIGGRTIHMYHTEG
                     AGGGHAPDIIRVAGEMHCLPSSTNPTNPFTVNTFDEHLDMTMVCHHLNPAIPEDVAFA
                     ESRIRTQTIAAEDVLHDIGAISMLGSDSQGMGRIHEVICRTWQLASKMKEQRGALPGD
                     RPGFGDNARILRYIAKYTINAARTFGISGHVGSLEDGKLADIVVWRPAFFGIKPELVI
                     KGGFIAWGAMGDSAASLMTCEPILMRPQWGAFGRAPAALSACFVHPLAIARDLSGTLG
                     LSKKMLPVGGTRGLTKRDMLWNDACPKISVDPETFDVFVDGALATCEPAREVPLARRY
                     MLR"
     gene            3891..4544
                     /gene="ureG"
                     /locus_tag="VN968_05430"
     CDS             3891..4544
                     /gene="ureG"
                     /locus_tag="VN968_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HXL28715.1"
                     /translation="MLHAVDEKSTAPPAVSPAARVGIGGPVGSGKTALIEALIPVLRR
                     RGVDLAVVTNDLVTKEDAERLRRSGLIDPARVSAVEAGACPHTVIREDPTLNIAAGDD
                     LEARFPGVELILFESGGDNLASTFSLDLVDWWMFVIDVAGGDDIPRKRGPGLLRCDLL
                     VVNKIDLAPYVGVELAPMLAEAGRVRAGRPVIATNARSGVGIETVADAILAAVLFKV"
     gene            4570..5364
                     /locus_tag="VN968_05435"
     CDS             4570..5364
                     /locus_tag="VN968_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HXL28716.1"
                     /translation="MVAERAGGRTVLRRQHVGYPFHVTRAFQLDRMRPDLATLYLQSA
                     SGGLYAADRLSLDLVVGAGSALNLTTQASTVVHDGRGHGSMMRQSVTVKDNAFCAIVS
                     DPYVLFPGADLRIATIATVAAGATLIMAEGFAAHDPHGRGGTFARFSTDTSIIGPDGT
                     PLVSDRGSVGGEELVAGCGALGGMTAAGSVLMIAPPDQVADIAEIEAAVDACGCLAGV
                     TAAPNHAGLAMRLLAPDSGTLIRGIEAAFHVAARAALGVDLAPRRK"
     gene            complement(5400..7724)
                     /locus_tag="VN968_05440"
     CDS             complement(5400..7724)
                     /locus_tag="VN968_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXL28717.1"
                     /translation="MSAALRSAPLRLISSQPEGAVRRVHLLMRQRDVLRPLFRIAWDN
                     LAARFDADELEGWAAGVLALADVNAGPSCLVALWELSRNQPTEEGIAPLIVAAHTAAN
                     VCRHAGAQGAAATIVSATVARRILGAGPSLARWWRTMDDLAQRAPESVEAAGSRMGEI
                     LSAGNIHAFESFVAVGLKLAAGDRRKRLAFFSLQEELARRLVARATAGVGFAEAERRT
                     KAFVTSLWGRPPLLRSFPDDTGQAPQRRAGIAGPLIRLPEIYRGVQGDAAYSLYRAAA
                     AHAQAHLVFGGSRFPVGSLKPLQIALVTMIEDARIELQAMRRLPGLRKLWSPYHVAVA
                     AGVATAPTLLARLARALFDRAYQDDDSFVARGRSMFEARLSRLEDPAISREIGMLLGN
                     DLGQMRVQFNAKTYLVEPVYRDDGLGLWDFGENAPSSGETLDLVVDAARIEQRDDPDQ
                     TSLHPDVVDDESKLGRARPVAPDQRGIVVAKYPEWDRAHGVERPEWTTVREVLARPGD
                     PRVVEEALERADVLRNRIRRLVRGVRVGRAIRLKRQLDGHDLDVDAMLEAGIALRMGQ
                     EPDPRVFRTTSSKHRDLAVLLLLDISESTRDRLASGVSVLEVERLAVAVLAEAMSALG
                     DPFSMLAFASNGRDDVEMTTVKAFHELYDRACVGRLAALSSGLSTRLGTALRHAGELI
                     SHARSFRKLVIVLTDGEPSDIDVADPLDLVEDARRAAVGLRARGIDGFGVVLGSNAMN
                     SAIRIFGRGNTMLVPRVEDLPSRLSELYFRLARR"
     gene            complement(7721..8521)
                     /locus_tag="VN968_05445"
     CDS             complement(7721..8521)
                     /locus_tag="VN968_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HXL28718.1"
                     /translation="MNLDAYLIVSEPFYQPVGDEIAVFEAAFSERLPVMLKGPTGCGK
                     TRFIEHMAWRLNKPLVTVAAHEDMTASDLAGRYLLEPDGTVWHDGPLTMAVRYGAICY
                     LDEIVEARQDTTVVIHPLTDARRILPLEKHNEIVPAHADFQLTISYNPGYQSAVKDLK
                     ESTKQRFLAIDFGYPPPAVEAAIVAREAGTNVDLAGTLVAIAERSRNLQGHGLDEGAS
                     TRMLIHAGRLVRAGLPLEAAVRSSIVLPITDNHDIREALSAAIEACIP"
     gene            complement(8816..>9224)
                     /locus_tag="VN968_05450"
     CDS             complement(8816..>9224)
                     /locus_tag="VN968_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HXL28719.1"
                     /translation="DDEVALPPPMLEWLEQAQKGKAGSKTTAMASFPDDEQLRLQYMG
                     KLGPNEFLLRLAKDTSTILPAEFSSELGLTTREGEVLSWLSKGKTNRDIAQILGLSPR
                     TVDKHLEQIYAKLGVENRTAAAAIATSTSRRNS"
BASE COUNT         1614 a         2934 c         3026 g         1650 t
ORIGIN      
        1 gagttcgagg tcgcgcatat gccgaacgcc aaattcatcc cggttccctc cgtctggggc
       61 cacttcgccg ggggccccgg caccaatccg aaggatgtcg aattcatcga tgcgaagcta
      121 aaggagcttc ttgcatcgta agcgccggcg ccggaattaa tcttccggtc cggtatcttc
      181 ccgggaagga tatctgagca tgctgagcgt cagtcattct tcgcaggtct aaccgctgaa
      241 agggcctcac gcgatgctgc gcatcgaccg tgtcttgggc agccgcctgg aagccgcatt
      301 ctcggaggcg attcaccgcc tcgaacatcg cggcgcggtg gatgtggtca atattccggt
      361 ggtcgacctc gaccgtcgcc gactgctggc gacgacgcgg gcgggcgaag aactggcgat
      421 tgcgttgccg cgtcaccaaa agctgttcga cggcgcggtc ctcctgatcg acgacgcgcg
      481 cgcgatcgtc gttcgcgccg ccacggagcg atggatgcgc ctcgaaccgc gttcgatcag
      541 cgacgcgatc gaactgggtt atcacgccgg aaatcttcat tggcgcgtgc gtttccaggg
      601 cgaggccttg ttcgttgcgc tggaaggccg gcccgaggac tacaccgcgc gtctcggcga
      661 gatgattgcc gcgcgccgcg tcggtgtcag cattctggac gaagaggtgc gcgacggggg
      721 cggcgaaaaa ggcacgccga aaggtgacgg cgaggccgcc acgaaagatc attttcacca
      781 cgatggccat cgatgatgtt gtcgatcctg caggcgatct ggcaagccga cggaacgttt
      841 ccgagcggat cgttcgcttt ctcctatggt gtcgagggcg ttgtcgccca gcacccccga
      901 ctggatggga ccgcgcttgc cgaattgacg accacgatca ttcgccagcg ctgggcgacg
      961 tgtgaccgga tcgcgctggt acaggcattt cacgccgcag gcgaccttcg cgccatcgcg
     1021 acgatcgatc gtgacgtgga ggcctcgacg ttcggcagca cgatgcgcga cggctcgcgg
     1081 cgtaacggcg gatccttcct ggcgtcgcat gcaaggctcg gcgacgccac ggcgttgcga
     1141 ttgcgcgatg cggtgcgcgc gggcatctgc cttggccata tcgccgtgat gcagggagcg
     1201 gtctggcagg cgatgggcct tgacgaaaaa ctggcgcagc tcgcttccgc ctatggcgtc
     1261 gcctccggtg tgacggccgc ggcggtgcgg cttggcgcga tcggtgcgct ggggggccag
     1321 aaggtcctgc gcggatgcct tcccttgatc gagacgctgg ttagccagcc ggtcccggag
     1381 gatgccgaac tttcgagctt cgttccattt ctagagatcg ccgccgcgcg gcacgcgcgc
     1441 gccgacctgc gcctgttcgt caattgagag ggtgtgaacg atgctgctga cgcccaccga
     1501 gctcgagcgt ttgacgatct tcaccgcggc cgaattggcg cgccggcggc gggcgaaggg
     1561 gctcaagctg aactatcccg aagccgttgc cattatcacc gacgaaatcc tcgaaggcgc
     1621 gcgggagggc cgatcggtcg ctgatctgat cggacatgga tcgacgattc tatcaaccga
     1681 tgacgtgctg tccggcatcg ccgcgatgat gaacatcatt cagatcgagt gcgtgtttcc
     1741 cgacggtacc aagctcgtga cggtccacga accgatccgg ccggcgcccg gttcgcaggc
     1801 tgatccggaa caccccggcc tcgtcaccgc agccgacggc agcatcgatc tcaacgcggg
     1861 gcgccgctcg ctcaaattga aagcccgaaa taccggcgat cggccgatcc agatcggttc
     1921 gcacttccat ttcttcgagg tcaacaaggc catggaattc gacagggccg ccgcgttcgg
     1981 catgcggctc gatattcccg ccggcaccgc ggtgcggttc gaacccggcg cctggaagga
     2041 agtctcgctc gtgacattcg gcggccgcag tgaattgacc gggctgaaca accttaccaa
     2101 cggattgagc tcggatccgg ccgtacgcga gggggcgctt gaacaggcga agaagcgggg
     2161 cttcaaaggg tcgtaacatg gcaagcatcg atcgccggga ctacgctgca ctctacggtc
     2221 cgaccaccgg agatgccgtt cggcttggcg acacggcgct gacggccgtg gtggagaagg
     2281 atcacgccgt ctatggcgac gaatgcctgc acggtggcgg caaaaccctt cgcgacggaa
     2341 tcgggatggc cgggatcacc agcgcggagg gcgcgctgga tttcctgttg tgcaacgtcg
     2401 tcgtgatcga tcccgtcgtc gggatcgtca aaggcgatct cggcatccgc catggccgca
     2461 tcgtcgggat cggcaaggcc ggcaatcctg ccatcatgga cggcgtcgat ccgcgcctga
     2521 tcgtgtccag cggcacaacc gtgcgcgact gcgaggggct gatcgcaacg ccgggcgcga
     2581 tcgacgttca cgttcatttc gacagcgcgg gcctggtcga gcatgcgctg gcgagcggca
     2641 tcaccaccat gatcggcggt tcgctcggac caatcaccgt cgggatcgat tccggcggtc
     2701 cgttcaacac cgggaaaatg ctgcaggccg cggaagcgtg gccgatgaac tttggctttc
     2761 tgggccgcgg caattcgcac cgcgtgccgc cgctggtcga acagctggaa accggtgtga
     2821 tgggcctgaa actgcacgag gactggggct cgatgccggc ggcgatcgat agctgtctga
     2881 aggttgccga cgagcatgac tttcaggtgc agatccatac cgacacgctg aatgaatccg
     2941 gcttcgtcga ggatacgctg gaggcgatcg gcggccgcac cattcacatg taccataccg
     3001 agggtgccgg cggcggccac gcgccggaca tcatccgcgt cgccggcgag atgcattgcc
     3061 tgccgtcgtc gaccaacccg accaatccct tcacggtcaa cacattcgac gaacacctcg
     3121 acatgaccat ggtttgccat cacctcaacc cggccattcc cgaggacgtg gcattcgcgg
     3181 agagccgcat tcgcacccag accatcgcgg ccgaggatgt gctccatgat atcggcgcca
     3241 tctcgatgct cggctccgac agccagggca tgggccgtat ccacgaggtg atctgccgga
     3301 cctggcagct ggcatcgaaa atgaaggagc agcgcggagc attgcctggg gatcgcccgg
     3361 gctttggcga caatgcccgc atcctgcgct acatcgccaa atacaccatc aacgcggcgc
     3421 gcacgttcgg gatatcaggc catgtcggct cgctcgaaga cggcaagctg gccgatattg
     3481 tggtgtggcg tccggccttt ttcgggatca agcccgagct tgtgatcaag ggcggcttca
     3541 tcgcctgggg ggcgatgggc gacagcgcgg cctcgctgat gacctgcgaa cccattctga
     3601 tgcgtccgca atggggtgcc ttcggcaggg cgccggcggc gttgtcggcg tgcttcgtgc
     3661 atccgctggc gatcgcgcgc gatctgtcgg gcacactcgg cctttccaag aaaatgcttc
     3721 cggtcggcgg cacccgaggg ctgacaaagc gcgacatgct gtggaacgat gcgtgcccca
     3781 aaatttcggt cgatcccgaa accttcgacg tcttcgtcga tggtgcgctt gccacctgcg
     3841 agccggcacg cgaagtgccg ctcgcgcgtc gctatatgct gaggtgacag atgctgcacg
     3901 cggtcgatga gaagagcacg gcgccgccgg ccgtatcgcc ggcggcccgg gtcggtatcg
     3961 gtgggccggt cggatccggc aagaccgcgt tgatcgaggc gctgatcccg gtgctgcggc
     4021 gtcgcggcgt cgatcttgcg gtggtaacca acgatctcgt caccaaggaa gatgcggaac
     4081 ggctccgccg ctccggcctc atcgatcccg cgcgcgtatc cgcggtcgag gcgggggcgt
     4141 gtccgcacac cgtgatccgc gaggatccga cgttgaatat cgccgccggc gatgacctcg
     4201 aggccagatt ccccggcgtc gaattgatcc tgttcgaatc cggcggcgat aatctcgctt
     4261 ccaccttctc gctcgatctg gtcgactggt ggatgttcgt gatcgacgtc gccggcggcg
     4321 acgacattcc gcgtaaacgg gggccggggc tgttgcggtg cgatctgctg gtcgtcaaca
     4381 agatcgatct ggcgccctat gttggcgtcg aacttgcgcc gatgctcgcc gaggccgggc
     4441 gcgtccgcgc cggccggccg gtgatcgcca ccaacgcgcg atccggcgtt ggaatcgaga
     4501 cggttgccga tgcaattctt gccgcagtcc ttttcaaggt ttgaggccgg ccgcaaggcg
     4561 gaagttcgct tggtcgccga acgcgccggc gggcgaaccg tgctccgtcg ccagcatgtc
     4621 gggtacccgt ttcacgtcac gcgcgccttc cagctcgacc ggatgcggcc cgatctcgcg
     4681 acgctttatc tgcagtcggc ctccggcggg ctttatgccg cggaccggct gtcgctggat
     4741 cttgtcgtcg gcgccggctc agcgctcaac ctgaccacgc aagcctcgac cgtggtgcat
     4801 gatggacgag ggcacggttc gatgatgcgc caatcagtga cggtgaagga caacgctttt
     4861 tgcgccattg tttctgatcc ttacgtcctg tttcctggcg ctgatctccg cattgcaacg
     4921 atagcaaccg tcgctgccgg cgcaaccctg atcatggccg agggctttgc agcccacgat
     4981 ccgcatggtc gcggtggaac attcgcgcga ttttcgaccg atacaagcat catcggaccg
     5041 gatgggacgc cgctggtctc cgaccgtggc agtgttggtg gcgaagagct cgtcgccggc
     5101 tgcggcgcgc ttggcggaat gacggctgca ggttcggttc tcatgatcgc gccgcccgat
     5161 caagtggctg atatcgcgga gatcgaggcg gcggtggatg catgcggatg tcttgccggc
     5221 gtaaccgccg cgccgaacca tgccggcctt gccatgcgac tgctggcgcc ggatagcggc
     5281 acgctgatcc ggggtatcga agccgcattc catgttgctg cgcgcgcagc actcggggtc
     5341 gatctggcgc cgcgtcgcaa gtgaaacagc gatcaagcga ggcagggatc tggaccagtc
     5401 tagcgtcgcg ccagccggaa atagagttcg gacagccggc tcggcaaatc ctcgacgcga
     5461 ggcaccagca tggtgttgcc gcgtccgaaa atgcgaattg cggaattcat ggcgttcgat
     5521 cccaagacca caccgaagcc atcgatgccg cgtgcccgca gtccgaccgc ggcgcggcgc
     5581 gcatcctcga cgagatcgag cggatcggca acgtcgatgt cggacggctc gccatcggtc
     5641 aggacgatga caagttttcg gaaactgcga gcatgactga tcagctcgcc ggcgtggcgg
     5701 agcgcggtgc cgagccgcgt cgacaggccg gacgacaatg ccgccagccg cccgacgcac
     5761 gcccggtcat agagctcatg gaaggccttg acggtcgtca tctcgacatc gtcgcgcccg
     5821 ttcgaggcga acgccagcat actgaaggga tcaccaagcg cgctcatggc ttcggcgaga
     5881 accgcgaccg ccagccgctc gacctcgaga actgagacgc cggatgccag gcgatcacgc
     5941 gtcgactccg agatgtcgag cagcagcaaa accgcaaggt cacgatgctt cgaacttgtc
     6001 gtgcggaata cccgcgggtc gggctcctga cccatccgca gtgcgattcc ggcctccagc
     6061 atggcatcga catcgaggtc atgaccgtcg agttgccgct tcagccggat ggctctgccg
     6121 acgcgcacgc cgcgaaccag ccgcctgatc cggttgcgca gaacgtcggc gcgttcgagc
     6181 gcctcttcca cgacgcgcgg atcgccgggc cttgcgagaa cctcgcgtac cgtggtccat
     6241 tccgggcgtt cgacgccgtg cgcgcgatcc cattccggat atttcgcaac cacaatgccg
     6301 cgttggtccg gcgcgacggg ccgcgcccgg ccaagcttgc tttcgtcatc aactacatcc
     6361 ggatggaggg aggtttgatc cggatcgtcg cgctgctcaa tgcgcgccgc gtcgacgaca
     6421 agatcgaggg tttcgccgga ggagggcgcg ttctcgccga aatcccacag gccgagaccg
     6481 tcgtcgcgat agaccggctc gacgaggtag gtcttggcgt tgaactgcac acgcatctgg
     6541 ccgagatcgt tgcccagcag catgccgatt tcgcgactga tcgccgggtc ttccagacgc
     6601 gaaagccggg cctcgaacat cgagcgcccc ctggcgacaa aactgtcgtc gtcttggtaa
     6661 gctcggtcga acaaggccct ggccagccgc gcgagcaggg tcggggccgt ggcgacgccg
     6721 gccgccacgg cgacatgata gggcgaccat aatttgcgca atcccggcag ccggcgcatc
     6781 gcctgcaact cgatgcgggc atcctcgatc atggtaacga gcgcgatctg aagcggtttt
     6841 aagctcccga cggggaagcg ggacccaccg aacaccagat gcgcctgggc gtgcgccgcc
     6901 gccgctctat ataaagagta agcggcgtcg ccctgcacgc cacgatagat ctcgggcaag
     6961 cggatcaggg ggccagctat cccggcacgg cgttgcgggg cctggccggt gtcatcagga
     7021 aaactgcgca gcagcggcgg ccgcccccac aatgaggtga cgaaagcctt ggtccggcgc
     7081 tcggcctcgg cgaatccgac gccggccgtt gctcgtgcca ccagtcgccg cgcgagctct
     7141 tcctgcagcg agaagaaggc cagccgtttg cgcctgtcgc cggccgccag tttcaaaccg
     7201 actgcgacga agctctcgaa ggcatggatg ttgcccgccg acaggatttc gcccatccgt
     7261 gatcccgccg cctcgaccga ttccggcgcc cgctgcgcga ggtcgtccat ggtccgccac
     7321 cagcgcgcga ggctcgggcc ggcccccaga attcgccgcg cgacagtggc ggagacgatg
     7381 gtggcggcgg cgccctgtgc gccggcgtgg cggcaaacat tcgcagcggt gtgcgccgcc
     7441 acgatgagcg gtgcgatgcc ttcttcggtc ggctggtttc ggctgagctc ccagagcgcg
     7501 accaggcagg acgggccggc attgacgtcg gccagcgcca gcacgccggc tgcccagccc
     7561 tcaagctcgt cggcatcgaa gcgcgcggcg agattgtccc aagcgatgcg aaacaacggt
     7621 cggagcacat cacgctgccg catcagcaaa tgaacacggc gaacggcgcc ctccggttgc
     7681 gaggaaatca gccgcagcgg cgccgatcgc agcgcggcgc tcacggtatg caggcctcga
     7741 tggccgcgct gagcgcttcg cggatatcgt ggttgtcggt gatcggcagg acgatgcttg
     7801 accggaccgc ggcttcaagc ggcaggccgg cacgcaccaa ccgtcccgca tggatcagca
     7861 tccgggtcga ggcgccttcg tcgaggccgt ggccctgaag gttgcgggat cgctccgcga
     7921 tggcgacgag ggttccggca aggtcgacat tggttccggc ctcgcgcgcc acgatggccg
     7981 cttcgacggc cggcggcgga tagccgaaat cgatcgccag gaaacgctgc ttggtcgatt
     8041 ccttcagatc cttcaccgca ctctggtagc ccgggttgta ggagatcgta agctggaaat
     8101 cggcgtgcgc cggcacgatc tcgttgtgct tttccagcgg caggatccgc cgcgcatccg
     8161 tcagcgggtg gatcacgacc gtggtgtcct gccgggcttc gacgatttca tcgaggtagc
     8221 agatcgcgcc atatcggacc gccatggtca gcggaccatc gtgccagacg gtgccgtccg
     8281 gctccagcaa atagcggccg gcaaggtcgg aggcggtcat gtcttcatgc gcggcgacgg
     8341 tcaccagcgg cttgttcaga cgccaggcca tgtgttcgat aaaccgggtt ttgccgcagc
     8401 cggtcggtcc cttcaacatg accggcagac gctcgctgaa agccgcctcg aacaccgcga
     8461 tctcgtcgcc cacgggctga taaaagggtt ctgatacgat caggtaggca tcgaggttca
     8521 cgcgcatctc cacggatggt gcgtggggaa ggccgcccgc ccgcgcgaag acctccttcc
     8581 caccgcagtg ggttcggcaa gcatcgcctt atgggcgcgc cacagcctcg gagcgcgcaa
     8641 gcagacgttc gtcaaacgtc tcgatcatca ttagactgcc aatcgcgatc ttgcacaagt
     8701 gaccttgggc cagaattgga cggtaataag cttgcaccac cggaagcggc tgcgttcgga
     8761 ggcaggcagg gtcgcgaaac cgttttccga acctgcggcg accgcaattt ctgatttatg
     8821 aattgcgccg gctcgtgctg gtggcgatgg cggcggcggc ggtgcggttc tcgacgccga
     8881 gtttggcgta gatttgctcg agatgcttat ccaccgttct tgggctgagg ccgaggatct
     8941 gcgcgatgtc ccgattggtc tttcccttgg acaaccacga cagcacctcg ccctcacggg
     9001 tggtcaatcc gagttcactg gaaaattccg cgggcaggat ggtgctggta tcttttgcca
     9061 gccgcagcag gaactcattc ggaccaagct tgcccatgta ttgcagccgc agctgctcgt
     9121 cgtccggaaa cgacgccatg gccgtggttt tcgatccggc cttgcccttc tgggcctgtt
     9181 cgagccactc cagcatcggc ggcggcagcg cgacttcgtc atcg
//
LOCUS       DATNOI010000145        15833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2083593,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000145 DATNOI010000000
VERSION     DATNOI010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15833
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2083593"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1028)
                     /locus_tag="VN968_05455"
     CDS             complement(75..1028)
                     /locus_tag="VN968_05455"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_028174674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HXL28720.1"
                     /translation="MSAKNGSIKLVAGNSNPVLAQHISDWLHLPLTKAVVRRFADMEI
                     FVEIQENVRGSDVFIIQSTSFPANDHLMELLIITDALRRASARRITAVIPYFGYARQD
                     RKVGSRSPISAKLVANIITRAGVDRVMTLDLHAQQIQGFFDIPTDNLYASPVMVRDIR
                     ERFDLTKVMVVSPDVGGVARARGLAKRINAPLAIIDKRRERAGESEVMNVIGDVAGHT
                     CILVDDMVDSGGTLVNAADALLANGAKDVFAYISHGVLSGGAAARITGSKLKELVITD
                     SIQPTEAVRNAANIRTLSIASLIAEAIGRTASEESVSSLFD"
     gene            complement(1197..1766)
                     /locus_tag="VN968_05460"
     CDS             complement(1197..1766)
                     /locus_tag="VN968_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28721.1"
                     /translation="MLLAIACGLGGCAGGGAASGAYAMASSGSGPTVAFESIDGPPPQ
                     VFDRMVSVLDSESKLRNLSIVSREGSAAYRVRSYLSVQVNHGRATLAWVWDVYDRDQQ
                     RALRLAGEEPAGKAGRDAWAVADDLVLRKIAQAGLSGLSGMMSGTLPADTPPPAATPG
                     KDGPAVASTEPALPASGAFRVSALSYTAR"
     gene            1980..2981
                     /locus_tag="VN968_05465"
     CDS             1980..2981
                     /locus_tag="VN968_05465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HXL28722.1"
                     /translation="MRAFVLTGYGAISDNVRLAEIPGPVAGSGEVLIEVHAASLNPID
                     FKLVHGALKRISNYKLPRPIGFDASGIVISAGAGATRFKPGDAVYVRASRDTIGTFAE
                     QIALPEKFVALKPATITHAEAASLPLVGLTTLQCFGRAGARAGQRILIHAGSGGVGTF
                     AVQYADHLGLDVTSTTSAKNADFVRSLGADRVIAYDRANYLEQGGDYDIVYDTLGGAF
                     TLDAFKLVKRGGVVISLSGPPDRDFARREGAGWLVRVAVWLIGRKVYAAAAKAGAAYC
                     WSFTEASGDQLRDIAALVDKRAIRPVIDREFAFEQLAAALTYLEAGRARGKVVLKVR"
     gene            complement(3009..4124)
                     /locus_tag="VN968_05470"
     CDS             complement(3009..4124)
                     /locus_tag="VN968_05470"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HXL28723.1"
                     /translation="MAGERRKITVGLLFGGRSAEHEVSRASAANILRALDPDRYEVSP
                     IGIAKDGRWRVCDAGNGAGSGAAALVIPDNAPQIALIPGGGGSFVWCDSGQPRGGAVT
                     RFDVVFPVLHGPNGEDGTVQGALELADVPFVGSAVTGSAIAMDKDVAKRLMRDAGLPV
                     VPFITTTSLSPIDYAAAVAALGTSDLFVKPANMGSSVGVSRARSREEFAASCELAFRY
                     DHKVLVERAVSDAREIECSVLEESDGHIHASQLGEIVPASKHGFYSYQAKYIDADGAA
                     LLIPAKLSPQQARRLQELAVEVFRLLGCAGLARVDFFVKGDDMFVNELNTLPGFTSIS
                     MYPKLWEASGLSQTDLMDRLIAHALARHARRRALSSE"
     gene            complement(4245..6053)
                     /gene="yidC"
                     /locus_tag="VN968_05475"
     CDS             complement(4245..6053)
                     /gene="yidC"
                     /locus_tag="VN968_05475"
                     /inference="COORDINATES: protein motif:HMM:NF002353.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HXL28724.1"
                     /translation="MHDQNNVFYAIALSLFILITWQYFFATSFVGKPSAQKTSQLGTM
                     APDAAIRPQPQAIAPQAAAIPNPQRFQQFSRQDALARSQRVAIDTPRVKGSIALTGGR
                     IDDLSLVQYRETTDPKSPAVRLLSPSGGPQPFYAEFGWINSSGINLQLPASETEWHLE
                     GSDSLGIGRPVTLVWQNGEGLEFRRTISVDDKYLFNVRDEVVNNGQAAVVLAPYASIS
                     RHSPPPTLGYYLLHEGAIGVLGEQGLQEVTYKTLDDKKRMAFNIGRAWLGFTDKYWAA
                     ILLPETPSQLHAEFSASSLGALKTYQADYVQGAQTIAPGGTATATARLFAGAKEVAIL
                     DGYDKALNLDRFDLAIDWGWFRFITKPMFTLIDTIFRWVGNFGVAILAVTLMLKIAFF
                     PLANKAYASAAKMKALQPQLQSIRESFGNDRVKQQQATQELYRKEKINPIAGCLPTLV
                     QIPVFFALYKVLFVTIEMRQAPFFGWIRDLSAPDPTNVFNLFGLIPYDPVSLPLLGGF
                     LAVGAWPLLMGFTQWVQVRLTPASPDPTQSAVLNWMPVIFTFMLAKFSAGLVIYWTWN
                     NSLSVIQQSIVMRRNGVRIELWDKVRVMFGKRKAGD"
     gene            6465..6857
                     /locus_tag="VN968_05480"
     CDS             6465..6857
                     /locus_tag="VN968_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28725.1"
                     /translation="MGALSALAPRLLSLSALSLTMALAGCAQHSAQHEVKADPVEAAA
                     PPQGHPDFRIHRPNRALLISQPAPDCEFRGTELKTVDPDQWARLRLDYERQCYQRAEK
                     KVRDRLRLLQASSRCEIEPVRPPLATIR"
     gene            7146..7985
                     /locus_tag="VN968_05485"
     CDS             7146..7985
                     /locus_tag="VN968_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HXL28726.1"
                     /translation="MKTLYDLLGALPDDDAESLRTAFRQAAKATHPDINTDDPDAPLK
                     FRQIVRANAILSDADQRATYDRLLALTRRRPNWKLKRTIRKAASDAIKVACLSAVSIG
                     GYLVFEQVSKASIVPVKATEVATRARTEIAAVTPTIPIDTTGRDKPPDKLESAGVASD
                     AVAPSAAAPAVHTDSAQAMANAGPALDLTARDAKSYRERGIVAYLDGDLYRALADFDV
                     AIQIDPGFADAYIDRGIVFYRIHEFDRAFADIAQAKRIKNSSRTKTLPLAPRKVSSSS
                     ARN"
     gene            complement(8041..8811)
                     /gene="pgeF"
                     /locus_tag="VN968_05490"
     CDS             complement(8041..8811)
                     /gene="pgeF"
                     /locus_tag="VN968_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HXL28727.1"
                     /translation="MRLGSPLLSAIPGLRHAFFTREGGVSGGIYASLNGGLGSGDDPA
                     SVAENRRRMAEQIGVDPEHFLSVHQTHSPDVVVATGPWQGASRPLADAIVTRSEGLAI
                     GATAADCGPILLVDPNARVIGAAHAGWKGALTGIVESTVEAMEKLGAERSGIVAAIGP
                     LIRQHSYEVGREFVERFVQADAENTLFFIPAAREAHAMFDLAGFIRMRLENAGVLMID
                     DTGIDTYSDERFFSYRRSVHCGEPDYGRHVHAIALERE"
     gene            complement(8808..9878)
                     /locus_tag="VN968_05495"
     CDS             complement(8808..9878)
                     /locus_tag="VN968_05495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXL28728.1"
                     /translation="MPVWRYMELCLTHPQHGYYISRDPLGREGDFITSPEVSQMFGEL
                     LGLWAASVWKAIGSPSVLRLIELGPGRGTLMADALRALRVLPPLYQALSVHLVEINPV
                     LREKQKATLSSVRVIAWHNSIDEVPRGPAVIFANEYFDVLPIHQMVKRETGWHERTVE
                     LDGNGKLAFGFAAEPTPRFEVLLPPLVRAAPVGAVFEWRPDAEIMKIATRVRDQHGAA
                     LVIDYGHIRSDAGDTFQAIARHSFTDPLKNPGLADVTAHVDFQALVRAAEDLGARAHG
                     PVPQGEFLKRLGIETRALTLMAKTTHEISEDISSALKRLTGDGRGGMGSMFKVLAISE
                     PGLASLAGLNDQDQQSIGAELP"
     gene            complement(9935..10786)
                     /gene="lgt"
                     /locus_tag="VN968_05500"
     CDS             complement(9935..10786)
                     /gene="lgt"
                     /locus_tag="VN968_05500"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXL28729.1"
                     /translation="MPLLTIDFPVFDPIAIAIGPIAIRWYALAYIGGIVLGWLYARAL
                     IKNEKLWGGPAPITLLQMDDFILWVTVGIILGGRTGYVLFYNLPFFVQHPAEIFELWK
                     GGMSFHGGFLGCVAAVMLFCRKHDLPVLSLGDITTAVGPIGLFLGRIANFINSELWGR
                     PADSSVPWAMVFPNGGPLPRHPSQLYEATLEGIVLFTILALMIRFGALKRPGLILGSF
                     IAIYGLARIVGEFFREPDPQLGFLWGGLTMGMLLSVPMIIAGAIIIVVAWRRTNSQAP
                     DTIEGKP"
     gene            complement(10819..11760)
                     /locus_tag="VN968_05505"
     CDS             complement(10819..11760)
                     /locus_tag="VN968_05505"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXL28730.1"
                     /translation="MRLLAFSDIHNNLVAVRKLRALEKNTFDAIVVAGDIGNASAADC
                     FKILATFKCPVVYVYGNWDHELSYKHCFEPGCQLLQSNVVTIGNINFAGFSGCPTNWG
                     KNPIARKIHRRIEIENRAVVDAVRHRTTPAGEIRRTKAYRSYELQLQSAEHEVLRLNR
                     ESIRKALSKAGIDPGKCVVVTHERLARLSEEIPGVLLHLFGHLHKFSERTFKATKYVN
                     VAALDHPVSARPRVKEKWRRQDCRNFNAGNYTTIEINSAQAVTIRCLFLPHDYPNWIP
                     LKDRRYNGIEWVPEEATWTNASDPPLRQRELTWSSSP"
     gene            complement(11775..12509)
                     /locus_tag="VN968_05510"
     CDS             complement(11775..12509)
                     /locus_tag="VN968_05510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXL28731.1"
                     /translation="MIDRQVEIPTKDGHTTTFISHPERDGPHPVILFYMDAPGIREEL
                     RDMARRLATSGYYVMLPNMYYRSGVMELGPLSPDPESPERKRMFGFMNSINIPMVMED
                     TTALLAYADGQPAARKDMVGAVGYCMSGRYAVNAATHFPGRVKAAASIYGTHLATDQP
                     DSPHLAAGKTKAELYFGCAETDIYAPTEIIEKLQASMKGANAEVEIYPGTHHGFAFPK
                     RPVYDRAAAERHWERLLALYRRNLVS"
     gene            12709..13002
                     /locus_tag="VN968_05515"
     CDS             12709..13002
                     /locus_tag="VN968_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HXL28732.1"
                     /translation="MTQTSNRFFDEIGRLMNDAAGAAQGVKREVDTVVRNQAEKILRD
                     LDIVKREEFEAVKDMARLAREENEALKARIASLEAKLGVSPSAPDVGAMKTDG"
     gene            13271..14017
                     /locus_tag="VN968_05520"
     CDS             13271..14017
                     /locus_tag="VN968_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HXL28733.1"
                     /translation="MATVKELKATTRPAGGKGAARAERRAGRVPGVIYGNNQPPLTIS
                     VEDIELRQRILAGRFLTTIYDIDLEGRKHRVIPRDFHLDPVRDFPIHVDFMRLGEGAT
                     IRVSVPLRIQNAESAPGVKRGGTVNIVTHTIDLECSVDNIPQYVEADVSGLEISHSLH
                     LSDVKLPAGVRSLSREDVTLVTIVPPSGYAEELKAAAAAAAAGPAATATPGAAPAAGA
                     AAAPAAGGAAAAGAKAPAAGAKAPGGDKKK"
     gene            14039..14644
                     /gene="pth"
                     /locus_tag="VN968_05525"
     CDS             14039..14644
                     /gene="pth"
                     /locus_tag="VN968_05525"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXL28734.1"
                     /translation="MLLFVGLGNPGAKYAHNRHNIGFMAVDEIARRHGFAPWRRRFQG
                     ETAEGTLDRERVVLLRPSTFMNESGRAVQEAASFFKLSAGEITVFQDEIELPAAKVRV
                     KVGGGIAGHNGLRSISSHIGNDYRRVRIGVGHPGVKELVHGHVLSDFAKSDRPWVEAL
                     CEAIADNVGLLVTDKDSTFQNKVHLAMQAKGFIDKEDGGGS"
     gene            14803..>15833
                     /gene="ychF"
                     /locus_tag="VN968_05530"
     CDS             14803..>15833
                     /gene="ychF"
                     /locus_tag="VN968_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HXL28735.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTETAAAQAANYPFCTIEPNVGE
                     VAVPDPRLDKLAEIAKSGQIIPTRLTFVDIAGLVRGASKGEGLGNQFLATIREVDAVA
                     HVVRCFEDSDITHVEGKIAPLADIETIETELMLADLDSLEKRVDNLTKKAKGNDKDAK
                     EQLDLVNRALVLLREGRPARFLARKPEEERAFGMLGLLTSKPVLYVCNVEEGSAATGN
                     DFSKLVTDHAKKEGAVAVAISAKIEAEIATLSREERAEFLDTLGLEEAGLDRLIRAGY
                     QLLDLITYFTVGPKEARAWTIHRGTKAPAAAGVIHTDFEKGFIRAETIACADYVALGG
                     EAGARDAGK"
BASE COUNT         3041 a         4855 c         5011 g         2926 t
ORIGIN      
        1 ggtcccggct ctgcggagca gcgtgaagga cgctgcaccg catccgggac acgaggcagg
       61 agttgtctgt ggctctaatc aaacaagctc gacaccgact cttccgacgc ggtgcggccg
      121 atcgcttcgg cgatcagcga ggcaatcgac agcgtgcgga tgttggcggc gttgcgcacg
      181 gcctcggtcg gctggatcga atcggtgatc acaagctctt tcaacttgga gccggtgatg
      241 cgcgcggcgg cgccgccgga cagcacgccg tggctgatat aggcgaaaac gtccttggcg
      301 ccgttggcga gcagcgcgtc ggcggcgttc accagcgtgc cgccggaatc gaccatgtcg
      361 tcgaccagga tacaggtatg tccggcgaca tcgccgatca cgttcatgac ctcggattcg
      421 ccggcgcgtt cgcggcgctt gtcgatgatc gccagcggcg cgttgatacg cttggcgagg
      481 ccgcgcgcgc gcgccacgcc gccgacatcg ggcgacacca ccatgacctt ggtgaggtcg
      541 aagcgttcgc ggatatcgcg caccatcacc ggcgaagcgt agagattgtc ggtcggaatg
      601 tcgaagaagc cctggatctg ttgggcgtgc agatcgagcg tcatcacgcg gtcgacgccg
      661 gcgcgggtga tgatatttgc caccagcttg gccgaaatcg gcgaacgcga accgaccttg
      721 cggtcctgcc gggcgtagcc gaaataggga atcaccgcgg tgatgcgccg cgcggaagcc
      781 cggcgcaacg cgtcggtgat gatcagcaat tccatcaaat ggtcgttggc cggaaacgac
      841 gtcgattgga tgatgaacac gtccgagccg cggacgtttt cctggatctc gacgaagatt
      901 tccatgtcag cgaagcgccg gaccacggcc ttggtcagcg gcaaatgcag ccaatcggag
      961 atgtgttgcg ccaggacagg attggagttg ccggcgacga gcttgatgga gccgtttttg
     1021 gccgacatcg acccttctcc ccgcgccttg ctggatacga tgttcagcat atcgggatac
     1081 ccacaaaaaa ggcgcgtttc gacgagcgag gtgatatcaa ggagatggct cggctggcaa
     1141 cccgtcctgt caggttttcc tgcgcaaaat cgcgggtttg gcgactattt gtatcgttaa
     1201 cgggcagtgt aactcagcgc gctgacgcgg aatgcacccg aggcgggcag agccggttcc
     1261 gtgctcgcca ccgccgggcc gtccttgccc ggcgtggcgg cgggcggcgg cgtgtccgcc
     1321 ggcagcgttc cgctcatcat gccgctgagg ccgctcaatc cggcctgcgc gattttccgc
     1381 aagaccagat cgtcggccac ggcccaggcg tcgcgtccgg ccttgccggc cggctcctcg
     1441 ccggccaggc gcagcgcgcg ctgctggtcg cggtcgtaga cgtcccagac ccaggcgagg
     1501 gtggcgcggc cgtggttgac ctggaccgaa agatagcttc gcacgcgata agccgccgag
     1561 ccttcgcgcg atacaatcga caggttgcgc agcttcgact cgctgtcgag cacgctcacc
     1621 atccggtcga acacctgcgg cggcgggccg tcgatcgatt cgaaggcgac cgtgggaccg
     1681 ctgccgctac tggccatcgc ataggcgccg ctcgcggcac cgccgccggc gcagccgccg
     1741 agtccgcacg cgattgccag cagcatggct gcaaccgcgg cgcgcggtgc ggtccgggtg
     1801 cccgcgacgg cccggaacag ggttgcaatg gtgtccttca tcgaggggag cgatatcgtt
     1861 aaaatgtgtt aaggtctagg gcagggagcc tttagcgctt tgaaaagatg atttttaagc
     1921 ggtcccgctc gctctcaaac atttgacatt tgaattctcc agccccgcag gattggccga
     1981 tgcgcgcatt cgttctcacc ggctatggcg caatatcgga taatgtcagg cttgccgaaa
     2041 ttcccggacc ggttgcgggt tcgggtgaag tcctgattga agttcatgcc gccagcctca
     2101 atccgatcga cttcaagctg gtgcacggcg cgctgaagcg gatttcaaac tacaaattgc
     2161 cgcggccgat cggtttcgat gccagtggca tcgtgatatc ggcgggcgcc ggcgcaaccc
     2221 gattcaagcc gggcgatgcg gtctatgtcc gtgcatcgcg cgacaccatc ggcacgtttg
     2281 cagaacagat tgcgcttccc gaaaaattcg tcgcgctcaa gccggccacg atcacgcatg
     2341 cggaggccgc gtcgttgccg ctggtcgggc tgaccacgct gcagtgtttc ggccgcgccg
     2401 gcgcgcgcgc gggccagcgc atcctgatcc acgccggctc cggcggcgtc ggcaccttcg
     2461 cggtccagta tgccgatcat ctcgggcttg acgtcacctc gacgacgagc gcgaagaacg
     2521 cggatttcgt gcgatcgctc ggcgccgaca gggtgatcgc ctatgatcgc gcgaactatc
     2581 tggagcaggg cggcgattac gatatcgtct acgacacgct cggcggtgcg ttcacgctcg
     2641 atgcgttcaa gctggtcaag cgcggcggcg tcgtgatctc gctcagcggc ccgccggacc
     2701 gcgatttcgc gcggcgcgag ggcgccggat ggcttgtccg cgttgcggtc tggctgatcg
     2761 gacgaaaggt ctacgccgcg gccgccaagg ccggcgcggc atattgctgg tccttcaccg
     2821 aggcgagcgg cgatcaattg cgcgacatcg cggctctcgt cgataaacgc gcgatcaggc
     2881 cggtgatcga tcgcgaattc gcattcgaac aattggcggc ggcgctgacc tatctcgaag
     2941 cgggccgggc gcgcggcaag gtcgttctga aagtgcgtta gccgcttctc gcgtcgatgt
     3001 cgcgactctc actccgaact cagcgcgcgg cgccgcgcat gacgcgcgag cgcatgcgcg
     3061 atcagcctgt ccatgagatc ggtttgggac aatccgctgg cttcccaaag cttgggatac
     3121 atgctgatcg aggtgaaccc cgggagcgtg ttcagttcgt tcacaaacat gtcgtcgcct
     3181 ttgacgaaga aatcgacccg ggcgaggccc gcgcagccga gcagccgaaa tacctcgacg
     3241 gccagctcct gaagacgacg ggcctgctgc ggggaaagtt ttgccggaat cagcagggcg
     3301 gccccatcgg cgtcgatata tttggcctga tacgaataga agccgtgctt gctcgccggc
     3361 acgatctcgc cgagctgtga agcatgaatg tggccgtcgg attcttccag gacagaacat
     3421 tcaatctccc gcgcgtcgct cacggcccgt tcgaccagga ctttatgatc gtagcggaac
     3481 gcgagttcgc acgacgccgc gaattcctcg cgcgagcgtg cgcgcgagac accgaccgac
     3541 gaacccatat tggcaggctt tacgaacaaa tcgctcgtcc ccagcgcggc gacggccgcg
     3601 gcgtagtcga tgggagacaa ggatgttgtc gtgatgaacg gcaccacggg aaggccggca
     3661 tcacgcatca ggcgcttggc gacatccttg tccatcgcga tggcagaacc ggtcaccgcc
     3721 gaacccacga acggcacgtc cgccaattcg agcgcgcctt gcacggttcc gtcttctccg
     3781 ttcggcccgt gcaggacagg gaatacaacg tcgaagcggg ttacggcgcc gcctctcggc
     3841 tggccgctgt cgcaccacac gaaactgccc ccgccgcctg gaatcagcgc gatttgcggc
     3901 gcgttgtcgg ggattaccag cgcggcggcg ccgcttcccg cgccgttacc ggcatcgcag
     3961 acacgccagc gaccatcctt cgcgatgccg atcggagaaa cctcataacg gtcggggtcc
     4021 agggcgcgaa gaatgttggc ggccgaggct ctggagactt catgttcagc cgagcggcca
     4081 ccgaacaaca gcccgaccgt gatcttgcga cgctctccag ccatcgttcc accctgtgcc
     4141 attgggccgt catgacagcc gtcagctcga accgaggtcg cagattaaga ctcaatggct
     4201 ggcaaaatgc aaagccgggc cgcagccgcg atcggatcgc ggctctaatc gccggccttg
     4261 cgttttccga acatcacccg gactttgtcc cagagttcga tccggacacc attcctgcgc
     4321 atgacgatgc tctgctgaat caccgacagg gaattattcc aggtccagta gatcaccagg
     4381 cccgccgaga acttggccag catgaaggtg aagataaccg gcatccagtt caacacggcc
     4441 gattgcgtcg gatccggcga tgccggagtg agcctgacct gcacccactg ggtgaagccc
     4501 atcagcagcg gccaggctcc gaccgccaga aacccgccaa gcagaggcag agacaccggg
     4561 tcatacggga tcagtccgaa aagattgaac acgttggtgg ggtcgggggc ggacagatcc
     4621 ctgatccagc cgaagaaagg cgcctgacgc atctcgatgg tgacaaacag gactttgtag
     4681 agcgcgaaga aaaccggaat ctgaaccagc gtcggcaggc acccggcgat cgggttgatc
     4741 ttctccttcc tgtaaagctc ctgtgtcgcc tgttgttgct tcaccctgtc gttgccaaaa
     4801 ctctcgcgga tagactgaag ctgcggctgc agggccttca tcttcgccgc cgacgcatag
     4861 gctttgttgg cgagcggaaa gaaggcgatc ttgagcatca aggtcacggc caggatcgcc
     4921 acgccaaagt ttccgaccca tcgaaagatg gtgtcgatca aggtgaacat cggctttgtg
     4981 atgaagcgga accagcccca gtcgatcgca aggtcgaacc gatcaaggtt gagcgctttg
     5041 tcgtagccgt cgaggatcgc aacttccttg gcgccggcga acagccgggc ggtggcggtt
     5101 gcggtgcctc cgggcgcaat ggtctgcgct ccctgcacat agtcggcctg ataggtcttg
     5161 agcgcgccga gggagcttgc ggagaattcc gcgtggagct gcgaaggcgt ctcggggagc
     5221 aagatggcgg cccagtattt gtcggtaaaa cccagccacg cacggccgat attgaacgcc
     5281 atccgctttt tgtcatcgag ggttttgtag gtaacttcct gcaatccctg ctcgccaaga
     5341 accccgatgg cgccttcgtg aagcagataa tagccaagcg tcggtggcgg gctgtgacgg
     5401 gagatcgatg catagggagc cagaacgacg gccgcctggc cgttattgac gacctcgtcg
     5461 cggacgttga agaggtattt gtcgtcgacc gaaatcgttc ggcgaaattc cagcccctcg
     5521 ccattttgcc aaaccagcgt caccgggcgt ccgatcccga ggctgtcgga tccctccaga
     5581 tgccattcgg tttcggaagc gggcagctgc agattgatgc cggaagaatt gatccagccg
     5641 aactccgcat agaacggttg cgggccgccc gatggcgaaa gcagccggac ggccggcgat
     5701 ttgggatcgg tggtttcgcg atattgcacc agcgacagat cgtcgatccg gccgccggtc
     5761 aacgcaatcg agcctttgac ccggggcgta tcgatggcga cacgctgcga tctggcaagc
     5821 gcgtcttgtc gcgagaactg ctggaaccgt tgagggtttg gaatcgccgc ggcctgcggt
     5881 gcgatggctt gcggttgcgg tcggatggcc gcgtcgggcg ccatcgtgcc gagttgcgaa
     5941 gtcttttgcg cgctcggttt cccgacaaag gacgtcgcga agaaatactg ccaggttatc
     6001 aggatgaaaa gggatagcgc gatagcatag aagacattgt tctggtcatg catggtaagt
     6061 ccccgttgct atccggcgcg ttccgtccct tcgacggaag tcatcagatc attctagcat
     6121 attgccggcg aagcaagccc atgtgcgttt tcaccgcggg tacggcggcc atctactccc
     6181 cagcacatcg cgttgtcgcc gtgaggccat tcggcacgag gccgatttac atttgattca
     6241 tcgtaaatgt taaaatgaaa ttcgaattct tcatccatgc tcgccgcgta cggccatggg
     6301 gccgggcgcc tgttgccgcg cgaagttggt gttgcgatcg gcgctgcatg acggacgcgg
     6361 ccaacttgcc tcgttttgga tcgggaaaca tgagcatggc gatcgcgggt cgagcgccct
     6421 ctggtgagag tgcatcgcgt ggcgtgcgat cgggcgccgg gggcttgggc gcactgtccg
     6481 cgcttgctcc taggcttttg agcctctccg ccctgtcgct cacgatggcg cttgccggtt
     6541 gcgctcaaca ttcggcgcag cacgaagtta aagccgaccc cgtcgaggcg gccgcccctc
     6601 cccagggaca ccccgacttt cgaattcacc ggcccaaccg ggcgctgctg atatcgcaac
     6661 cggcccccga ctgcgagttc aggggcaccg agctcaagac ggtggatccg gatcaatggg
     6721 cgcgcctgag gctcgattac gagcgccaat gctaccaaag ggcggagaag aaggtccgcg
     6781 accgtttgcg gctgctgcag gcatcgagca gatgcgagat tgagccggtt cggcccccgc
     6841 tggcgacgat ccggtaacca gttccgccgc gacaggccga gttcatcacg gctggaccgg
     6901 atggtcattg gacctttcac cgttgcggtg cggtcatacc gacctcgcaa ggatccaggt
     6961 cgacccggag cgacccatac ccaccgcaaa tcccggtgtg gagagcggcg gtattgcgct
     7021 gcgatgtacg agtcatgttt tggccatact gaaacatcag tttttgagta tgtatggcct
     7081 tgtcagtcgg cgactccacg tcggaatttc tgagggggag cgtcagcgag agtaaccagt
     7141 caacgatgaa gactctctac gatttgctcg gcgcgcttcc ggacgatgac gccgaaagcc
     7201 tcaggacggc attccgccag gccgccaaag cgacccaccc cgatatcaat actgacgatc
     7261 cggatgctcc gctgaaattc aggcagattg tccgtgccaa tgccatcctg agcgatgcgg
     7321 accagcgtgc gacatacgac cggctgctgg cgctcacacg ccggcggccc aattggaagt
     7381 tgaagcgtac catccgcaag gccgcttccg atgcgatcaa ggttgcttgc ctttccgccg
     7441 tgtcgatcgg aggatatttg gttttcgagc aggtctcgaa ggcatccatc gttccggtaa
     7501 aagcgaccga ggttgcgaca cgtgcacgga ccgagatcgc cgcggtcacg ccgacgatac
     7561 caatcgacac gaccggccgt gacaagccgc cggacaagct ggagagcgca ggggtcgcca
     7621 gcgatgcggt cgcgccgagc gcggctgcac ccgcggtgca tacagacagc gcgcaggcaa
     7681 tggccaatgc cggtccggcg ctcgacctca ccgcgcgcga tgccaagtcc tatcgagagc
     7741 ggggaatcgt tgcgtacctt gatggcgacc tgtaccgcgc gctcgccgac ttcgacgtgg
     7801 cgattcagat cgatcccggc ttcgccgatg cctacatcga tcggggcatc gttttctatc
     7861 gtattcacga attcgaccgc gcgttcgccg acatcgctca ggcgaagcgt atcaagaact
     7921 cgagccggac caagactctt ccgttggcac cgcgcaaggt gtcgtcttca tcggccagga
     7981 attgaaccgg gtggaatgcc tgcgcttgcc cgtccggcac gccgccgtcg attgaaacaa
     8041 ttattcgcgc tccagcgcga tcgcgtgcac gtgccggccg tagtccggct cgccgcagtg
     8101 caccgagcgg cgatagctga agaagcgttc gtcggaatag gtatcgatgc cggtgtcgtc
     8161 gatcatcagg acgccggcgt tttcaagcct catccggatg aagccggcaa ggtcgaacat
     8221 cgcatgggcc tcgcgcgccg cgggaatgaa aaacaaggtg ttctccgcat ccgcctgcac
     8281 gaagcgctcg acgaattcgc ggccgacttc gtaagagtgc tggcggatca gcgggccaat
     8341 cgcggcgacg atgccgctgc gttcggcgcc gagcttttcc atcgcctcca cggtggattc
     8401 cacaatgccc gtcagcgcgc ccttccatcc ggcatgcgcg gccccaatca cgcgcgcgtt
     8461 cggatcgacc agcaggatcg gtccgcagtc ggccgcggtg gcgccgatgg cgagaccctc
     8521 gctgcgtgtg acgatggcat cggccagcgg ccgcgacgcg ccttgccagg gtccggtggc
     8581 caccacgaca tcgggcgaat gggtctggtg cacgctgaga aaatgttccg ggtcgacgcc
     8641 gatctgctcc gccatccgtc ggcgattttc cgcgacggaa gcaggatcgt cgccggagcc
     8701 gaggccgcca ttgaggctcg cgtagatgcc accggacacc ccgccctcgc gcgtaaagaa
     8761 cgcgtggcgc aggccgggaa tggcggagag cagcggcgat cccaacctca tggcagttcc
     8821 gccccgatgc tttgctgatc ctgatcgttc agccccgcca gcgaggcaag gccgggttcg
     8881 gagatcgcga gcaccttgaa catcgacccc atgccaccac ggccgtcgcc ggtcagccgt
     8941 ttcagcgcgc tcgaaatgtc ctcggaaatc tcatgcgtgg tcttcgccat cagcgtaagc
     9001 gcgcgggttt cgatgccgag ccgtttcaga aactcgccct gtggcaccgg gccatgggca
     9061 cgcgcgccga gatcctcggc ggcgcgcacc agggcctgaa aatcgacatg ggcggtgacg
     9121 tcggccagac cggggttttt cagggggtcg gtgaagctgt ggcgggctat ggcctgaaag
     9181 gtatcgccgg cgtcgctgcg aatgtgaccg taatcgatga ccagcgccgc gccgtgctga
     9241 tcgcggacgc gggtcgcgat cttcatgatc tcggcgtcgg gccgccattc gaacaccgcg
     9301 ccgaccggtg cggcgcgcac cagtggcggc agcaggactt caaagcgcgg ggttggttcg
     9361 gcagcgaaac cgaacgccag cttgccgttg ccgtcgagct cgacggtgcg ctcatgccag
     9421 ccggtctcgc gtttcaccat ctggtggatc ggcaggacgt cgaaatattc gttggcgaaa
     9481 atgaccgcgg gacccctggg cacctcgtcg atgctgttgt gccaggcgat gacacgcaca
     9541 ctggacagcg tcgccttctg cttttcgcgc agcaccggat tgatctcgac gagatggacg
     9601 ctcagcgcct gatagagcgg cggcagcacc cgcagcgcgc gcagcgcatc cgccatcagg
     9661 gtgccgcggc cgggtcccag ttcgatcagt cgcagcacgg atggcgaacc gatcgccttc
     9721 cataccgacg ccgcccacaa cccgagcaat tcgccgaaca tctggctcac ttcgggcgag
     9781 gtgatgaaat cgccctcgcg ccccagcgga tcgcgcgaaa tgtaatagcc gtgttgggga
     9841 tgcgtcaggc acagctccat gtaacgccag accggcatcg ggccggacga cttgatgagc
     9901 tgtttgagtt cggactggag cggcgaatac tcgctcacgg cttgccctcg atcgtgtcag
     9961 gcgcctggga attggttcgg cgccatgcca ccacaataat gatcgcgcct gcaatgatca
    10021 tcggcaccga tagcagcatg cccatggtca gcccgcccca caagaatccg agctgcgggt
    10081 cgggctcccg gaaaaactcg ccgacgatgc gcgcaagacc gtagatcgcg atgaagctgc
    10141 cgaggatcag gcccggccgc ttcagggcgc cgaagcggat catcagcgcc aggatcgtaa
    10201 acagcacgat tccctcaagc gtcgcctcat atagctggct ggggtggcgt ggcagcggtc
    10261 cgccattggg aaataccatc gcccacggca cgctggagtc cgccggccgg ccccacaact
    10321 cgctgttgat gaaattggca atgcgcccga gaaacaatcc gatcggcccg accgcggtgg
    10381 tgatatcgcc gagcgacagg accggaaggt cgtgcttgcg gcagaacaac attaccgcgg
    10441 cgacgcagcc caaaaagccg ccatggaacg acatgccgcc cttccacaat tcgaaaatct
    10501 cggcgggatg ctggacgaag aacggcagat tgtagaacag cacatagccg gtgcggccgc
    10561 ccaggatgat gccgaccgtc acccagagaa taaaatcgtc catctgcagc agcgtgatcg
    10621 gcgccgggcc tccccacagc ttctcattct tgatcagcgc gcgggcgtag agccagccga
    10681 gcacgatgcc gccgatatag gccagcgcat accagcggat agcgatcggc ccgatcgcaa
    10741 tggcgatcgg gtcgaacacc ggaaagtcga tggtgagaag gggcatcagg aaaatttcac
    10801 ggggggtatc cgaggtaatt atggcgaact gctccacgtc agttcacgtt gacgcagggg
    10861 tgggtccgac gcgttcgtcc atgtcgcttc ctccggaacc cactcgattc cgttgtatct
    10921 acgatctttc aatggaatcc aattcggata atcgtgcggg agaaacaagc accgtattgt
    10981 tacggcttga gcagagttga tctcgatcgt ggtgtagttg cctgcattga aattgcgaca
    11041 atcctgtctt cgccattttt ccttgacgcg aggtctggcg gagacggggt ggtcgagtgc
    11101 tgcgacatta acatatttcg tcgccttgaa agtgcgttcg gaaaacttat gaaggtgacc
    11161 gaaaagatgc aggagtacgc cgggaatttc ctcgctcaac cgagcaaggc gttcatgggt
    11221 cacgaccaca catttgccgg ggtcgatccc cgccttgctt aacgccttgc ggatgctttc
    11281 ccggttgagt ctcagcactt catgttcggc cgactgaagt tgaagttcgt agctccgata
    11341 ggcctttgta cggcgaattt cgccggccgg agttgtccga tgccttacgg cgtctaccac
    11401 ggccctattt tcgatttcga tcctgcgatg gatttttcga gcgatcggat tttttcccca
    11461 gttcgtcgga cacccgctaa agccggcgaa attgatattg ccgatcgtga cgacattcga
    11521 ctgaagcagc tggcagccgg gttcaaaaca atgcttgtag ctcaattcat gatcccaatt
    11581 gccatataca tacacgacgg ggcatttgaa cgtcgcgaga atcttgaagc aatcggcggc
    11641 gctggcgttg ccgatatcgc cggcaacgac gatcgcatcg aatgtatttt tctccagcgc
    11701 ccgcaatttt cggacagcca ccagattgtt gtggatgtcc gaaaatgcga gcaacctcat
    11761 cttgcttccc gtggctacga tacgaggttg cggcggtaga gcgccaacag ccgttcccaa
    11821 tgccgctcgg cggcggcgcg atcgtaaacc ggccgcttcg gaaacgcgaa gccgtgatgg
    11881 gtgccgggat aaatctcgac ctcggcattg gcgcccttca ttgacgcttg cagcttctcg
    11941 atgatctccg tcggcgcgta gatatcggtc tcggcgcagc cgaaatacag ttcggccttg
    12001 gtcttgcccg cggccagatg cggactgtcg ggctggtcgg tggccagatg cgttccgtag
    12061 atcgaggccg cggccttgac gcgccctgga aaatgcgtcg cggcgttgac ggcatatcgc
    12121 ccgctcatgc agtagccgac ggcaccgacc atgtccttgc gcgcggccgg ctggccgtcg
    12181 gcgtaggcga gcagggcggt cgtatcctcc atcaccatcg ggatgttgat cgagttcatg
    12241 aagccgaaca tccgcttgcg ctcgggcgat tccggatccg gcgacaacgg cccaagctcc
    12301 atgacgccgg agcggtagta catgttgggc agcatcacat aatagcccga cgttgccagg
    12361 cgacgcgcca tgtcgcgcag ttcctcgcgg atacccggcg cgtccatata gaacaggatc
    12421 acgggatgcg gaccgtcgcg ctcgggatgg ctgatgaagg tggtggtgtg gccgtccttg
    12481 gtcggaattt cgacttgccg gtcaatcatg gagttttcgc tcgggttttg caggtttctt
    12541 gatttgggcg ttcaatacga ttgcaaggaa accgcaacat gacaaggtaa ctcgccgctt
    12601 tgagagattt gccgcatcag ggaacgacag cggccttgaa gcgcgccgtc cggatcgcca
    12661 ttgtctgacc agcccttcca gttcgcagcc gaccggagat tccccaagat gacccagacc
    12721 agcaatcggt ttttcgacga gatcgggcgc ctgatgaatg acgccgccgg cgccgcccaa
    12781 ggcgtcaagc gtgaggtcga tacggtggtc cgcaatcagg ccgaaaaaat cctgcgcgac
    12841 ctcgacatcg tcaagcgcga ggagttcgag gcggtcaagg acatggcgcg gctggcgcgg
    12901 gaggaaaacg aggccctcaa ggcgcggatc gcttcgctgg aagcaaaact cggcgtttcg
    12961 ccgtcggcgc cggatgtcgg cgcgatgaag acggatgggt agggcctccc gtcgtattcg
    13021 gcgtcatacg cgggcttgac ccgcgtatcc atcgatcttc ggaagatatt gcgaggaaga
    13081 tagatcaccg ggcccggtga tgacgacagg gctaaatccg cacaaaaatc cccgcatttc
    13141 cttgtcattt tgctcctttg gggctagaac cccgccacgt ccgcagcccc cgcacccctg
    13201 gaggcttgct gcggcggcga acgggccgcg atgcggcctt taactttttg gaaaacaagg
    13261 acttaatcca atggcgaccg tcaaggaatt gaaggcgacc acacgtccgg caggcggcaa
    13321 gggggccgca cgggcagagc gtcgcgccgg cagagtgccc ggagtaatct atggcaacaa
    13381 ccagcccccc ctgaccattt cggtcgagga tatcgaactg cgccagcgta tcctggccgg
    13441 ccgctttctc accacgattt acgacatcga tctggagggc aggaagcacc gcgtgattcc
    13501 gcgcgacttc catctcgatc cggtgcgcga ttttccgatc catgtggatt tcatgcggct
    13561 cggcgagggt gccaccatcc gcgtcagcgt tcccctgcgt atccagaacg cggaatccgc
    13621 acccggcgtg aagcgcggcg gcaccgtcaa catcgtcacc cacaccatcg acctggaatg
    13681 ctcggtcgat aacattccgc aatatgtcga ggccgatgtc agcggtctgg agatcagcca
    13741 ctcgctgcat ctgtcggatg tcaagctgcc cgccggcgtg cggtcgttgt cgcgcgaaga
    13801 cgtcacgctc gtgaccatcg tgccgccgtc cggctacgcc gaagaactca aggcggctgc
    13861 ggctgccgct gctgccggtc cggcggctac ggctaccccc ggcgctgctc cggcggccgg
    13921 cgctgctgcg gctccagcgg cgggtggcgc cgccgcggcg ggcgccaagg cacctgcggc
    13981 cggcgccaag gcgcctggcg gcgacaagaa gaagtaaccg gacgcgggat gccgcgtcat
    14041 gctgctcttt gttggtctcg gcaatcccgg cgcgaaatac gcgcataacc ggcacaacat
    14101 cggtttcatg gccgtcgatg aaattgcgcg gcgtcatggt ttcgcaccat ggcgccggcg
    14161 ttttcagggc gaaaccgcgg aaggcacgct cgatcgcgag cgagtggtgc tgctgcgccc
    14221 gtcaaccttc atgaacgaat cggggcgtgc agttcaggag gccgcgagtt tcttcaagct
    14281 gtcagccggc gagatcaccg tgtttcagga tgaaattgaa ctgccggccg ccaaggtgcg
    14341 ggtcaaggtc ggcggcggca ttgccggaca caacggtctg cgctcgatct cgtcgcatat
    14401 cggcaacgac taccgccgcg tgcggatcgg ggtcggccac cccggcgtca aggaactggt
    14461 gcacggccac gtgctgtccg atttcgccaa gagcgaccgc ccctgggttg aggcgctctg
    14521 tgaggcgatc gcggacaatg tcggactgct ggtgacggac aaggattcga cgttccagaa
    14581 caaggtgcat ctggcgatgc aggccaaggg atttatcgac aaggaagacg gtggcggaag
    14641 ttaaagcacc gccgtcgtcg ccggacttga tccggcgatc catcattcaa gaacgatgga
    14701 tgcgcgggtc aattagcagg gaagacggcg cttcgcgcct tttgcccgcg tatgacgctc
    14761 cgaatagact cactcgccgc gcgcgaagga tcgggaagca cgatgggatt caaatgcggg
    14821 attgtcggat tgcccaatgt cggcaagtcg acgctgttca atgcgctgac ggaaacggcg
    14881 gcggcgcagg cggcgaatta tccgttctgc accatcgagc cgaatgtcgg cgaggtcgcg
    14941 gtgcccgatc ccaggctcga caagctcgcg gagatcgcca aatccggcca gatcattccg
    15001 acgcggctga cctttgtcga catcgcgggg ctggtgcgcg gcgcctcgaa gggcgagggc
    15061 ctcggcaacc agtttctcgc caccatccgc gaggtcgatg cggtcgcgca tgtggtgcgc
    15121 tgctttgagg attccgacat cacccatgtc gaaggcaaga ttgcgccact ggcggatatc
    15181 gagaccatcg agaccgaact gatgctcgcc gatctcgaca gcctcgagaa gcgggtcgac
    15241 aacctcacca agaaggcaaa aggcaacgac aaggacgcca aggaacagct cgacctcgtc
    15301 aaccgcgcgc tggtgctgtt gcgcgagggc agaccggcgc ggtttctcgc gcgcaagccc
    15361 gaggaggagc gcgccttcgg catgctcggc ctgctcactt ccaagccggt gctgtatgtc
    15421 tgcaacgtcg aggaaggctc ggcggcgacc ggcaatgatt tttccaagct ggtgacggat
    15481 cacgccaaga aggaaggcgc ggtcgcggtt gcgatctccg ccaagatcga agcggagatc
    15541 gcgacgctgt cgcgcgagga gcgcgccgag tttctcgaca cgcttggact ggaggaggcc
    15601 ggtctcgacc gcctgatccg cgccggctac cagctgctcg atctcattac ctatttcacc
    15661 gtcgggccca aggaagcccg cgcctggacc atccaccgcg gcaccaaggc gccggccgcc
    15721 gccggcgtga ttcataccga tttcgaaaag ggttttatcc gggccgaaac catcgcctgc
    15781 gccgattacg tcgcattggg cggcgaggcc ggcgcccgtg acgccggcaa gtt
//
LOCUS       DATNOI010000146         2647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_485030,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000146 DATNOI010000000
VERSION     DATNOI010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485030"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2647)
                     /locus_tag="VN968_05535"
     CDS             complement(<1..>2647)
                     /locus_tag="VN968_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HXL28736.1"
                     /translation="LEGTQIVLEELPDAPTKGMRSNLPLLDRQQAFGYTQEDITILMT
                     PMASTGEEANGSMGNDTPISALSDKPKQLFTYFKQNFAQVTNPPIDPIREELVMSLVS
                     IIGPRPNLFDLQGLAGTKRLEVRQPILTDADLEKIRSISEIADTHFKSRTLDTTFHAG
                     FGAAGLEQVLDELCARAEAAVREGVNIIILSDRMAGSDRIPIPSLLACAAVHHHLIRT
                     GLRTSVGLVVESGEPREVHHFACLAGYGAEAINPYLAFETIISMRDRLPSALDDYEIV
                     KRYIKSIGKGLLKVMSKMGISTYQSYCGAQIFDAVGLKADFVSKYFVGTHTRIEGVGL
                     AEMAEETARRHADAFGEAPVYKTALDVGGEYAYRTRGEDHAWNAESVSTLQHAVRGNS
                     LERYKAFAKILNEQSERLLTLRGLFKIKTAEDEKRKPVPLDQVEPAKDIVKRFATGAM
                     SFGSISREAHTTLAIAMNRIGGKSNTGEGGEESDRFKPMPNGDSMRSAIKQVASGRFG
                     VTTEYLVNADMAQIKMAQGAKPGEGGQLPGHKVDAVIARVRHSTPGVGLISPPPHHDI
                     YSIEDLAQLIYDLKNVNPDSLVSVKLVSEIGVGTVAAGVAKARADHVTIAGFEGGTGA
                     SPLTSIKHAGSPWEIGLAETHQTLVRERLRSRIVVQVDGGFRTGRDVVIGALLGADEF
                     GFSTAPLIAAGCIMMRKCHLNTCPVGVATQDPVLRKRFTGQPEHVINYFFFVAEEVRE
                     IMAALGYRTFDEMIGQTQMLDQSTLVAHWKAKGLDFSKLFVRQKELPGQKIYHAEPQN
                     HHLEAVLDRKLIEKAQAALDRGAPVKIDMEINNTDRSAGAMLSGTVAKIYGHAGLPHD
                     TIQVGFKGTAGQAFGAWLTRGITFDL"
BASE COUNT          437 a          827 c          876 g          507 t
ORIGIN      
        1 gagatcgaag gtgatgccgc gcgtcagcca cgcgccgaac gcctgacccg cggtgccctt
       61 gaagcccacc tggatggtgt cgtgcggcaa gccggcatgg ccatagattt ttgcaaccgt
      121 ccccgacagc atcgcgccgg cgctgcggtc ggtgttgttg atctccatgt cgatcttgac
      181 cggcgcgccg cggtcgagcg ctgcttgcgc cttctcgatc agcttgcggt ccagcaccgc
      241 ttccaggtga tggttctgcg gctccgcgtg atagatcttc tggccgggca gttccttctg
      301 gcggacgaac agctttgaga agtcgagccc cttcgccttc cagtgcgcca ccagggtgga
      361 ctggtcgagc atctgggtct ggccgatcat ctcgtcgaag gtgcggtagc cgagcgcggc
      421 catgatctcg cggacttcct cggcgacgaa gaagaaatag ttgatgacgt gctcgggctg
      481 gccagtgaag cgcttgcgca gcaccgggtc ctgcgtcgcg acaccgaccg gacaagtgtt
      541 gagatggcac ttgcgcatca tgatgcagcc ggccgcaatc aagggcgcgg tggagaagcc
      601 gaattcgtcg gcccctaaca gcgcaccgat caccacgtcg cgtccggtgc ggaagccgcc
      661 gtcgacctgc accacgatgc ggctgcgcag ccgctcgcgc accagggtct ggtgggtttc
      721 ggcaagcccg atttcccagg ggctgccggc gtgcttgatc gaggttaagg gggatgcacc
      781 ggtgccgccc tcgaagccgg caatcgtgac atggtcggcg cgcgccttgg caacgccggc
      841 ggcaaccgtg ccgacgccga tttcggagac gagcttcacc gataccaggc tgtccggatt
      901 gacgtttttg aggtcataga tcagctgcgc cagatcctcg atcgaataga tgtcgtgatg
      961 cggcggcggc gagatcagcc cgaccccagg ggtcgaatgc cgcacccggg caatgacagc
     1021 gtccaccttg tggccgggca attgtccgcc ctcgccgggt tttgctccct gcgccatctt
     1081 gatctgcgcc atgtcggcgt tgacgagata ttcggtcgtg acgccgaagc ggcccgaggc
     1141 gacctgcttg atcgccgagc gcatcgaatc gccattcggc atcggcttga agcgatcgct
     1201 ctcctcgccg ccttcgccgg tgttggactt gccgccgatc cggttcatcg caatcgccag
     1261 cgtggtatgg gcctcgcgcg agatcgagcc gaacgacatc gcgccggtgg caaagcgctt
     1321 gacgatgtcc ttggccggtt cgacctggtc gagcggcacg ggcttgcgct tctcgtcctc
     1381 ggcggtcttg atcttgaaca ggccgcgcaa ggtcaacagc cgctcggact gctcgttgag
     1441 gatcttcgcg aacgccttgt agcgctccag cgaattgccg cgcaccgcat gctgcagcgt
     1501 cgagaccgac tcggcgttcc aggcgtgatc ctcgccgcgg gtgcggtagg catattcgcc
     1561 gccgacgtcg agcgcggtct tgtagaccgg cgcctcgccg aacgcgtcgg cgtggcggcg
     1621 cgcggtttcc tcggccatct cggcaagacc cacgccttcg atgcgggtgt gggtgccgac
     1681 gaaatatttc gagacgaaat ccgccttcag tccgacggcg tcgaagatct gcgcgccgca
     1741 ataggactga taggtggaga tgcccatctt cgacatcacc ttcaacaggc ccttgccgat
     1801 cgacttgatg tagcgcttga cgatctcata atcgtcgagc gctgacggca gccgatccct
     1861 catcgagatg atggtctcga atgccagata gggattgatc gcctcggcgc cgtagccggc
     1921 gaggcaagcg aaatgatgca cctcgcgcgg ctcgccggat tcgacgacaa gcccgaccga
     1981 ggtgcgcagg ccggtgcgga tcaggtgatg atgcacggcg gcgcaggcca gcagcgaggg
     2041 gatcggaatc cggtccgagc ccgccatgcg gtcggacagg atgatgatgt tgacgccctc
     2101 gcggactgct gcttccgcgc gcgcgcagag ttcatcgagc acctgttcga gtccggccgc
     2161 gccgaagccg gcatggaagg tggtgtcgag ggtgcgcgac ttgaaatggg tgtcggcgat
     2221 ttccgagatc gagcggatct tttccagatc ggcgtccgtc aggatcggct ggcgcacttc
     2281 aagccgcttg gtgccggcca agccctgcag atcgaacagg ttcggccgcg gcccgatgat
     2341 cgagaccagg ctcatgacca gctcttcgcg gatcggatcg atcggcggat tggtgacctg
     2401 cgcgaagttc tgcttgaagt aggtgaacag ctgcttcggc ttgtccgaca gcgccgagat
     2461 cggcgtgtcg ttgcccatcg agccgttggc ctcttcgccg gtcgaggcca tcggcgtcat
     2521 caggatggtg atgtcctcct gggtgtagcc gaacgcctgc tgccgatcga gcagcggcag
     2581 gttggagcgc atgcccttgg tcggcgcatc cggcaattcc tccagcacga tctgggtgcc
     2641 ctcgagc
//
LOCUS       DATNOI010000147        17206 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1156576,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000147 DATNOI010000000
VERSION     DATNOI010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17206
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156576"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VN968_05540"
     CDS             complement(<1..167)
                     /locus_tag="VN968_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HXL28737.1"
                     /translation="MIHVCSLAALPDTVKATGASHVLTVMANVDQVQRPESVLPANHL
                     KVSMDDITEQM"
     gene            complement(228..845)
                     /locus_tag="VN968_05545"
     CDS             complement(228..845)
                     /locus_tag="VN968_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HXL28738.1"
                     /translation="MTAGKLSAAEIPPRVWQRMLSGRRLDLLDPSPLDVEIADIAHGL
                     ARVARWNGQTSGAHIFSVAQHTLLVEAVMREQTPRVDVRLRLAALLHDAPEYVIGDMI
                     SPFKAVLGGDYKAVEKRLLSAIHIRFGLPPVLAEEITRQIKAADRGAAYLEATQLAGF
                     AEAEAKRLFGRDPGLPPASVRDYLTPWTAARAEKRFLARFKTLLP"
     gene            complement(842..1468)
                     /locus_tag="VN968_05550"
     CDS             complement(842..1468)
                     /locus_tag="VN968_05550"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HXL28739.1"
                     /translation="MTRSARLHADGLVRCPWPGEDPFYMAYHDTEWGVPEYDDRALYE
                     KLILDGFQAGLSWITILRKRDNFRRAFDDFVPEKIARYNAKKLHALMNDAGIVRNRAK
                     IEGTVASAKGYLKIMEDGPGFSKFLWDFVDGRPKVNNFKTTASVPASTPLSIKISKEL
                     AARGFKFVGPTIVYAFMQATGMVNDHLVTCFCHETCAGKQRIPRLKVK"
     gene            complement(1465..2346)
                     /locus_tag="VN968_05555"
     CDS             complement(1465..2346)
                     /locus_tag="VN968_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HXL28740.1"
                     /translation="MKAAFLPDRGVVKISGIDARDFLNGLVTTDVTLLRPGLGRFGAL
                     LTPQGKITVDFLIAEAPSGHGGGFLIDCPRALAQGFADKLGFYKLRAKVAVENLSDSL
                     GVVAAWDGDPAVMPDLSFADPRHAALGWRILMPVELAQKVADLIGADLVDSGAYDAHR
                     IASGVPRGGLDFMYGDAFPHETNMDRLHGVDFEKGCYVGQEVVSRMQHRGTARTRTIR
                     IILDGPSPEPGAAILAGDKSVGTMGSAAGPHGLALIRVDRVADALAAGLPLTSGGLAI
                     RVAEPNDLLIAPKQTVA"
     gene            2508..3842
                     /locus_tag="VN968_05560"
     CDS             2508..3842
                     /locus_tag="VN968_05560"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXL28741.1"
                     /translation="MNQRFDTILKSGTVVNQDGEGVRDIAVTDGRIAAIGGLGQASAA
                     EVIDCKGLHILPGVIDTQVHFREPGLTHKEDLETGSRSAVMGGVTAVFEMPNTDPLTA
                     TEQTFADKVKRGHHRMHCDFAFFIGGTRDNVQNLPELERAPGCAGVKVFIGSSTGALL
                     VEDDESLRRIFQVIRRRAAFHAEDEYRLNDRKGLRVEGDPRSHPVWRDETAALLATQR
                     LVKLAHETGKRIHVLHISTKEEIEFLRDHKDVASCEATPHHLTLAAPECYERLGTRAQ
                     MNPPVRSADHRDGIWRGIGQGIIDVLGSDHAPHTLEEKAKTYPASPSGMTGVQTLVPL
                     MLDHIHAGRLSLQRFVDLTSAGPARLYNIAVKGRIAAGYDADFTIVDLKRSETITNKW
                     VASRAGWTPYDGVRVTGWPVGTFVRGRRVMWQGELVTSSTGEPVRFLETLKA"
     gene            complement(3975..4241)
                     /locus_tag="VN968_05565"
     CDS             complement(3975..4241)
                     /locus_tag="VN968_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28742.1"
                     /translation="MSHPASFRTARDPLPDQEQKQAALGYLNEAWAEARHDGVDGDCL
                     AQASLFAAFAELVGTYGEDAVATYAEGLPARIRNGEFSTTRARQ"
     gene            complement(4428..4805)
                     /locus_tag="VN968_05570"
     CDS             complement(4428..4805)
                     /locus_tag="VN968_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02301 family protein"
                     /protein_id="HXL28743.1"
                     /translation="MLKHFLAALILISACHAGPARAQDAAAPFDGDLQRLAEILGTLH
                     YLRGICGTNEGPKWRNEMQALIDAETPSGDRRARMIAGFNRGYNGFQQTYRTCTPAAS
                     VAIRRYIEEGSKISRDLTARYAN"
     gene            complement(4887..5318)
                     /locus_tag="VN968_05575"
     CDS             complement(4887..5318)
                     /locus_tag="VN968_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXL28744.1"
                     /translation="MSTVSPVQPSHPQLAVSAAIFRDGKVLLVRRARSPAKGFYSLPG
                     GRVEFGEPLHTALHREVDEETALKIEIIGLAGWHEVMPGTSGGGHYLIMSFAARWIAG
                     EPVLNDELDDFRWLAPDAVGDLKVTGGLFEVIQSARALLGG"
     gene            complement(5565..6080)
                     /locus_tag="VN968_05580"
     CDS             complement(5565..6080)
                     /locus_tag="VN968_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28745.1"
                     /translation="MTSTASQPVRSDRPMPQPTAPDKEARAAQLRAANINPRTGLATD
                     YLNHFNEAIMLLEMIPDMPECADDFLSWHPLSYAEHFTASHFKARDLAIEAYESADAN
                     VRAEFDNITAAMTSILTAVSAAMREAKQDKTRSTLAEQATAWVKPLVALAGGIINGRG
                     DETDVDYIMTH"
     gene            6143..6910
                     /locus_tag="VN968_05585"
     CDS             6143..6910
                     /locus_tag="VN968_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HXL28746.1"
                     /translation="MCGRFVITSPPAALRQIFGYIEQPNFPPRHNIAPTQPIPVVIVE
                     NGIRHFRLMRWGLLPAWVKDPGKFTLLINARVETVLEKPAFKNAMKRRRCLIPADGYY
                     EWQVSEKRKRPHFVHRRDGQPIGLAGLAETWIGPNGEELDTVAILTAPASADLAVLHH
                     RVPVTIEPDDFERWLDCGDDDAEKVMALLVAPGEGRFVWHEISTRVNRAANDDAQLIL
                     PITAEEMAAEEPRPAKKAALRKPAPAASEDDGQGSLF"
     gene            complement(7034..7615)
                     /locus_tag="VN968_05590"
     CDS             complement(7034..7615)
                     /locus_tag="VN968_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase"
                     /protein_id="HXL28747.1"
                     /translation="MTVSDRVRVNDVRMLSDNHYILKTTTFEWRRNSGEWQTQARETY
                     DRGNGAALLPYNLAQRSVVLVRQFRYPAFVNGYDDLLIEAAAGLLDNESPEVRILAEA
                     EEETGYRLGKVQKVFEAFMSPGAITEKLHFFVAEYEAGMRVGSGGGIASEGEDIEVLE
                     LPIDSALAMIGDGRIVDAKTIMLLQYAALNIFR"
     gene            complement(7637..8089)
                     /locus_tag="VN968_05595"
     CDS             complement(7637..8089)
                     /locus_tag="VN968_05595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HXL28748.1"
                     /translation="MSSTAPAPLAIAEIADADIADVIALWQRCGLTRPWNDPAADIAL
                     ARNGANAAVLAGRDGGGIVASVLVGHDGHRGWVYYVAVDPDCRHKGYGRLIMDAAEGW
                     LRGRGIEKLQLMVRPDNSQVQAFYQSLGYLEQERIIYAKWLDGREPTP"
     gene            complement(8092..9519)
                     /locus_tag="VN968_05600"
     CDS             complement(8092..9519)
                     /locus_tag="VN968_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXL28749.1"
                     /translation="MNILQPASPPLSPELIARFRKIVGDKYAVTDAADIAPYLTEERD
                     LFHGRSPLVLRPGSTAEVSAICKLATEYRIALVPQGGNTGLVGGQTPHNGEVVVSMLR
                     MNEIRDIDTASNTMTCEAGVVLQVAQQRASEVDRLFPLSLGAEGSCTIGGNLSTNAGG
                     TTALAYGVAREMALGLEVVLADGRILNGLSKLKKDNTGYDLRNLFIGAEGTLGIITAA
                     TLKLFPRPRAVETAFVGLTSPAQALRLLSISQNEAAGSLTSFELLSDIAVDFSVRHGI
                     DIRDPLEAKHPWYVLMELSSSRDDARAALESILAKGLDEGIIDDAVIAANLSQRAAFW
                     KLRDEMSAAQKPEGGSIKHDISVPVAAVPDFIEEANAAVVKLIPGSRPVPFGHLGDGN
                     IHYNVSQPVGAEPADFLARWHEVNAVVFAIVMRMGGSISAEHGIGVLKRDELPDVKDK
                     VAIGLMRGIKAMLDPSGIMNPGKVL"
     gene            complement(9567..10559)
                     /locus_tag="VN968_05605"
     CDS             complement(9567..10559)
                     /locus_tag="VN968_05605"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXL28750.1"
                     /translation="MNVSLTTQILAANEAAVALAARSLANGGLVAFPTETVYGLGADA
                     TNPKAIARLYQAKGRPAFNPLIATVRDLEAAKRIARFDTTAIKLAEAFWPGPLTLVLP
                     KTSDCAVAEFATAGLDTIAIRVPAHRVARDILRAFGGPVVAPSANLSGHVSPTTAAHV
                     QSDLAGRIDLIVDGGPVAVGVESTIVGCFDEAVLLRPGGLPRGDIERLLGRKLVQPPG
                     DADSDSGRPLAPGMLASHYAPRTLVRLNACNVEPGEALLAFGPVAVSGVDAATSVMNL
                     SARGDLDEAAANLFGYLRALDTRGAHTIAVMPVPHHGLGEAINDRLRRAAMGRD"
     gene            10628..10704
                     /locus_tag="VN968_05610"
     tRNA            10628..10704
                     /locus_tag="VN968_05610"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10662..10664,aa:Pro,seq:cgg)
     gene            12483..12959
                     /locus_tag="VN968_05615"
     CDS             12483..12959
                     /locus_tag="VN968_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28751.1"
                     /translation="MKALSLGLLLAITTLSSVHAQRSPESLPAVTVDCYQSFAAKAPL
                     PELSSKEMAIDGGSFETPLIRYRLQVADKTTLKIIKDPTRPAFRTEYGKSVWEIKRDF
                     TNRHQIVGWREELPGGIVRLFTFDFEDLLLSTIDVSPASSSAAGVELHVMKCSKSI"
     gene            complement(13010..14293)
                     /locus_tag="VN968_05620"
     CDS             complement(13010..14293)
                     /locus_tag="VN968_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HXL28752.1"
                     /translation="MVKPFPSKTHIGNHMLHPETLMLNYGYDPQLSEGAVKPPVFLTS
                     TFVFRTAEDGQDFFDFVSGRREPPEGMGAGLVYSRFNHPNSEIVEDRLSVYERTEKCA
                     LFSSGMAAIATTILAFVRPGDVIVHSQPLYGGTETLFARTLAGLSIGAVGFADGIDEA
                     GVRRAAEHAVRRGRVAMIFIETPANPTNGLVDIAMVRRVAEMIGQAQGHTPIIACDNT
                     LLGPVFQRPIEHGADLSLYSLTKYIGGHSDLIAGAALGSRALMKDVKALRGAIGTQLD
                     PHSCWMISRSLETLSIRMEKADRNARLVADYLRDHAKVADVHYLAHHEEASPAGRLFA
                     RQCTGAGSTFSFDIVGGQAAAFKFLNALQIFKLAVSLGGTESLASLPASMTHSGVPAD
                     IRQKIGVLDSTIRLSIGIEHPSDLIADIAQALNEA"
     gene            14570..15658
                     /locus_tag="VN968_05625"
     CDS             14570..15658
                     /locus_tag="VN968_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXL28753.1"
                     /translation="MALIKKVETGFYRIPLSVTLTDSTHGEMKAFELITIRLRDSEGA
                     EGVGYTYTVGRNGRAVADLLRREIPELIEDQEADDTEAIWQRIWWALHYGGRGGPSVL
                     ALSALDIALWDLKARRANLPLWRLLGGFDRSVPCYAGGIDLDLSIEDLLKQTDDNLGK
                     GFRAIKMKVGRPDLASDVARVRAMRDHLSGGFPLMADANMKWTVEAAIRAAKAFQPFD
                     LTWLEEPTIPDDVPGHRRILAQGGVPIAAGENLRSLWEFKSYIVSDAVSYPEPDVTNC
                     GGVTSFMKIARLAESFNLPVTSHGAHDITVHLLAACPNRSYLEAHGFGLDRYIEHPLI
                     LEEGMAIAPMRPGHGISFDWNALVPLSA"
     gene            15850..16653
                     /locus_tag="VN968_05630"
     CDS             15850..16653
                     /locus_tag="VN968_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXL28754.1"
                     /translation="MTSFSLAHLTVLNVPPPEMIRVAARTGYDCVGLRMIAVTDTTPG
                     YPLMHEPAMMRATKAALADTGVRVLDIEFVKITPEIDVAGLERFVAAGAELGAKHVIT
                     APYDSELARLADRLGAITDLCARYGLRTVLEFFPWTVVPDLEAAVRMVEAAGRTETGI
                     LVDTLHFDRSGSRVEQLDRISPARLPFVHVCDAPVQDTYTTEELLHAGRAERLPPGEG
                     GIDIGGILRHMPQGIPVALEVPMTAMNAAEGAEAVALRVRQAAERLLAA"
     gene            complement(16639..>17206)
                     /locus_tag="VN968_05635"
     CDS             complement(16639..>17206)
                     /locus_tag="VN968_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL28755.1"
                     /translation="SWQQFYTGIFHLQRSPQLEIADPLGLVQSQAILNPDKRFRIVLN
                     GSAATRTMSARFLTEFFGAGVQHIAFECDDIFETVAGMRSAGASFLKIPDNYYDDVES
                     RFELDPAVATRMRENQILYDRDETGEFFQVYTHAFDERFFFEIVQRRGYQGFGAPNAS
                     VRLAVQTREARPITVPRPDSASDQSGRQ"
BASE COUNT         3286 a         5198 c         5435 g         3287 t
ORIGIN      
        1 tccatctgtt cggtgatgtc gtccatcgat actttcagat ggttggcggg gagtaccgac
       61 tccggccgct gcacctggtc gacattggcc atcacggtga ggacgtggct ggcgccggtg
      121 gccttgacgg tgtcgggaag cgcggcgagc gagcagacgt gaatcatggc ggtccttttg
      181 ttggtgcaat tataggcttg cccccgccac ggcgaaagca tcgatgttca tggaagcaat
      241 gtcttgaacc gcgccaggaa ccgcttttcc gccctggccg cggtccaggg cgtcaggtaa
      301 tcgcgcacgc tggccggcgg caggccggga tcgcggccga acaggcgctt cgcctcggct
      361 tcggcaaagc ctgccagctg ggtcgcctcg agatacgccg cgcccctgtc ggcggccttg
      421 atctgtcgcg tgatctcctc ggccagcacc ggcggcaatc cgaagcggat gtggatcgcc
      481 gaaagcagcc gcttttccac cgccttataa tcaccgccaa gcaccgcctt gaacggcgag
      541 atcatgtcgc cgatgacata ttccggcgca tcgtgcagca gcgccgcgag cctcaatcga
      601 acatccacgc gcggcgtctg ctcacgcatc acggcttcga ccaatagcgt gtgctgcgcc
      661 accgaaaaaa tatgtgcgcc actggtctgg ccgttccagc gtgcgacccg cgccaggccg
      721 tgggcgatat cggcgatttc gacgtccagc ggcgaggggt ccaggagatc gagccgccgc
      781 cccgacaaca tccgctgcca gacgcgtggc ggtatttcgg ccgcggataa ttttccagcc
      841 gtcatttgac tttgaggcgc gggatgcgct gttttccggc gcaggtttca tggcaaaagc
      901 aggtcaccag atggtcgttg accatgccgg tggcctgcat gaaggcatag acgatggtcg
      961 ggccgacgaa tttgaatccg cgcgcggcca gttctttcga tatcttgatc gacagcggcg
     1021 tcgacgccgg cacgctggcc gtggtcttga agttattgac cttcggcctg ccgtcgacga
     1081 aatcccacag gaatttcgag aagcccgggc cgtcctccat gatcttcaga tagcccttcg
     1141 cgctcgcgac cgtgccctcg atcttggcgc ggttgcggac gatgccggcg tcgttcatca
     1201 gcgcatgcag ctttttggcg ttgtagcgcg cgatcttttc agggacgaaa tcgtcgaagg
     1261 cgcggcgaaa gttgtcgcgc ttgcgcagga tcgtgatcca cgacagcccg gcctgaaaac
     1321 cgtcgaggat gagcttttcg tacaacgcgc ggtcatcata ttccggcacg ccccattcgg
     1381 tgtcgtgata ggccatgtaa aacggatctt cgccgggcca tggacaccgc accaggccat
     1441 cggcatgcag gcgcgccgac ctcgtcatgc cacggtctgc ttcggtgcga tcagcaaatc
     1501 gttgggctcg gcaacgcgga tggcgaggcc gcccgatgtc agcggtagcc cggcggcaag
     1561 cgcgtcggcc acgcggtcca ctctgatcag tgcgaggcca tgggggcctg ccgccgagcc
     1621 catggtgccc accgatttgt cgccggccag aatggctgcg cccggctccg gcgacgggcc
     1681 gtccagaatg atccgtattg ttcgggtgcg ggcggtaccg cggtgctgca tccgcgacac
     1741 cacctcctgg ccgacataac agcccttttc gaaatcgacg ccgtgcaggc gatccatgtt
     1801 ggtttcgtgt gggaacgcgt cgccatacat aaagtcgaga ccaccgcgcg gaacgcccga
     1861 ggcgatgcga tgggcgtcgt aggcgccgct gtcgaccaga tcagcgccga tcagatcggc
     1921 gaccttttgc gcaagttcca cgggcatcag aatccgccag ccgagcgcgg catggcgcgg
     1981 atcggcgaac gaaagatcag gcatcacggc gggatcacca tcccacgccg ccaccacacc
     2041 gaggctgtcc gagagatttt cgaccgcgac cttggcgcgc aatttataga agccaagctt
     2101 gtcagcaaaa ccttgcgcga gcgcgcgcgg gcagtcgatc aggaacccgc cgccatggcc
     2161 ggaaggcgct tcggcaatca ggaaatccac cgtgatcttg ccctgcggcg tcagcaacgc
     2221 gccgaaccgg ccgaggcccg ggcgaagcag cgtaacgtct gtggtgacga ggccattgag
     2281 aaagtcacgg gcgtcgatgc cgctgatctt gaccacgccg cggtcgggaa gaaacgctgc
     2341 tttcataagg ggttctctga caaagcttct tttgcaagtt cgcttggttc ttttgcaagt
     2401 tcttggggaa acgccagcgg cggatatcac atggcaacgt aagacgctaa agtatcggga
     2461 acaaggcccg cgcgacaccg gttccgtaaa gcgggtactg aggacccatg aatcaacgat
     2521 ttgataccat tctgaaatcc ggcaccgtgg tcaatcagga tggcgagggc gtgcgcgaca
     2581 tcgccgttac ggatggccgc attgcagcaa tcggcgggct tggacaggcc tccgccgccg
     2641 aggtaatcga ctgcaagggc ctgcacatcc tgcccggtgt gatcgacacc caggtgcact
     2701 ttcgcgagcc gggcctcacc cacaaggagg acctggaaac cgggtcgcgc agcgccgtca
     2761 tgggcggtgt cacggccgtg ttcgaaatgc cgaacaccga tcccttgacc gccacggagc
     2821 agactttcgc cgacaaggta aagcgcggcc atcaccgcat gcattgcgat tttgcgttct
     2881 tcatcggcgg cacccgcgac aatgtgcaga atctgccgga gctggagcgc gcgcccggct
     2941 gcgccggggt caaggtgttc atcggctcgt cgaccggcgc gctattggtc gaggatgacg
     3001 aaagcctgcg gcggatcttt caggtgatcc ggcgccgcgc cgcctttcat gccgaggacg
     3061 agtaccgcct caacgatcgc aaggggttgc gcgtcgaagg cgatccacgc tcgcatccgg
     3121 tgtggcgcga cgagaccgcg gcgctgctgg cgacgcaacg cctggtcaag ctcgcccacg
     3181 agaccggcaa gcggattcac gtgctgcata tctcgaccaa ggaggagatc gaattcctga
     3241 gggatcacaa ggacgtcgcc tcctgtgaag cgacgccgca tcatctgacg ctggcggcgc
     3301 ctgaatgcta cgagcggctc ggcacccgcg cgcagatgaa tccgccggtg cgctcggccg
     3361 atcaccgcga cggcatctgg cgcggcatcg ggcagggcat catcgacgta ctcggctccg
     3421 atcacgcgcc gcatacgctg gaggaaaagg ccaaaaccta tccggcctcg ccgtcgggca
     3481 tgaccggggt gcagacgctg gtgccgctga tgctcgatca catccatgcg gggcgattgt
     3541 cgctacagcg ctttgtcgat ctcaccagcg ccgggcccgc gcggctgtac aacatcgccg
     3601 tcaagggccg gatcgcggcg ggctatgatg cggactttac catcgttgat ctcaagcgca
     3661 gcgagaccat caccaacaaa tgggtggctt cgcgcgcggg ctggacgccc tatgacggcg
     3721 ttcgcgtcac cggctggccg gtcgggacct tcgtgcgcgg ccgccgcgtg atgtggcagg
     3781 gcgagctggt gacgtcctcg accggcgagc cggtgcggtt tctggagacg ctgaaggcgt
     3841 aattgattgc ttactcgtca ttgcgagcca acggatcggc gcgaagcgcc gccccgttgg
     3901 ctcgcaatga cggctactgg gtcgcccggt cgagccggtc aagccaggcg atgacggctg
     3961 tgcttaatgc gaactcactg cctggcccgc gtggtcgaga actcgccatt gcggatgcgg
     4021 gcggggaggc cctccgcata ggtcgcgacc gcgtcttcgc cgtaggttcc gaccagttcg
     4081 gcgaaggcgg cgaacaggct tgcctgtgcc aggcagtcgc catcgacccc atcgtggcgc
     4141 gcctcggccc aggcctcgtt gagatagccg agggctgcct gcttctgttc ctgatcgggc
     4201 agcggatcgc gggcggttcg aaacgacgct ggatggctca tgaaactcgg caggctgttg
     4261 cgcgatccag ggcggagcgc gtcgaatgcg cgggggttta ccatgcgatt cggggtcgcc
     4321 tagaccagtt ctttaagaaa ggttaacggc gctgattgcc atgaaatttg tgccgaactc
     4381 gaagtttgcg cggtgccggg cggcgaagag aactttccgg taaacagcta gttggcgtag
     4441 cgggcggtca ggtcccgcga gatcttcgag ccttcctcga tgtaccggcg gatcgcgacc
     4501 gaggccgccg gcgtgcaggt ccggtaggtc tgctggaagc cgttatagcc gcggttgaag
     4561 cccgcgatca tgcgggcgcg gcgatcgccc gagggggttt cggcatcgat cagcgcctgc
     4621 atttcgttgc gccatttggg cccttcattg gtgccgcaaa tgccccgaag atagtgcagg
     4681 gtgccgagga tctcggccag ccgctgcagg tcgccgtcga acggcgcggc cgcatcctgg
     4741 gcccgcgcag ggccggcatg gcatgccgag atgagaatga gagcggccag aaagtgcttg
     4801 agcatgaagg ggatccggga cgtccgtgat atgccttctc aatgcgctgg aagcaaggct
     4861 cgaagggctt gggacgcgag cccgagtcag cccccgagca gggcgcgggc ggactggatg
     4921 acctcaaaaa ggcctccggt gaccttgaga tcgccgaccg catccggagc tagccacctg
     4981 aaatcgtcga gttcgtcgtt caggaccggc tcgccggcga tccagcgcgc cgcgaacgac
     5041 atgatcaaat agtgcccgcc gccactggtt cccggcatca cctcgtgcca tccggcgagg
     5101 ccgataatct cgatcttcag cgcggtttcc tcgtcgactt cgcgatgcag cgcggtgtgc
     5161 agcggctcgc cgaattcgac ccggccgccg ggcagcgaat agaaaccctt ggccggcgac
     5221 cgcgcgcgcc gcaccagcag gaccttgccg tcacgaaaga tcgccgcact gacggcaagc
     5281 tggggatgcg acggctggac cggtgacacc gtggacatca gagcatgatc cggaaaagca
     5341 tgccctcggg ctccgtcccg atgggtggaa gccggttttc ccttgcgacc aacgcggttg
     5401 gcgcggagat catgctcaaa caaggagacg ggatcatgat tcgattctac ctgatcgggt
     5461 catgatctag atgtgatccg gaaaaatgga aaccggtttt ccaaaaagat catgctcaaa
     5521 caaagatgag atcgtgatcc gattcaatct aatcggatca tgatctaatg cgtcatgatg
     5581 tagtcgacgt cggtctcgtc gccgcggcca ttgatgatgc cgccggccag cgcgaccagc
     5641 ggcttgaccc aggcggtggc ctgttcggcc agcgtggagc gggtcttgtc ctgcttcgcc
     5701 tcgcgcatgg cagcgctcac cgcggtgaga atggaggtca ttgcggcggt gatattgtcg
     5761 aattcggcac gaacgtttgc gtcggcggat tcataggcct cgatcgcgag atcgcgtgcc
     5821 ttgaaatggg acgccgtgaa atgctcggcg taggacaaag ggtgccagct caggaaatcg
     5881 tccgcgcatt ccggcatgtc cgggatcatc tcgagcagca tgatggcttc gttgaaatgg
     5941 ttcaggtaat cggtggcgag accggtgcgg ggattgatgt tcgcagcccg caactgcgcg
     6001 gcccgcgcct ccttgtcagg ggccgtcggt tgcggcatgg ggcgatcgga tcgcacgggt
     6061 tgggaagccg ttgatgtcat ctgccgcagt gttaacggtt ggggttaaaa ggtgctgaac
     6121 gcatacccat ctggacctca gaatgtgtgg acgcttcgtt attacctcgc cgccggcggc
     6181 cttgcgccag atctttggct atatcgagca gcccaacttc ccgccacggc ataatattgc
     6241 gccgacccag ccgattccgg tggttattgt cgagaacggc atccggcatt tccgcctgat
     6301 gcgctggggc ctgctgccgg cctgggtcaa ggatcccggt aaatttaccc tgctgatcaa
     6361 tgcacgcgtc gaaaccgtgc tggaaaaacc ggccttcaag aacgccatga agcggcggcg
     6421 ctgcctgatc ccggcggacg gctattatga atggcaggtt tcggaaaagc gcaagcggcc
     6481 tcatttcgtc catcgccgcg acggacagcc gattggcctc gccgggttgg cggaaacctg
     6541 gatcggcccc aacggcgagg aactcgatac cgtggccatc ctcaccgcac cggccagcgc
     6601 agatctcgcg gtattgcatc accgggtgcc ggtgaccatc gagcccgatg atttcgagcg
     6661 ctggctggat tgcggcgatg acgatgctga aaaggtgatg gcgctgctgg tcgcgcccgg
     6721 cgaaggccgt ttcgtctggc acgagatctc gaccagggtc aatcgcgccg ccaatgacga
     6781 cgcgcaactg atcctcccca ttacggcgga agagatggca gcggaggagc cgaggccggc
     6841 gaaaaaggct gccttgcgca aacctgcgcc ggcggcgtct gaagatgatg ggcaggggtc
     6901 gttgttttga aaatcctatc gctcgtcatg cccggccttg tgccgggcat ccacgttttc
     6961 ttccttgaat gtttgtgaag acgcggatgg ccgggacgag cccggccatg acgagtttac
     7021 aaaattgtgt gcttcaccga aaaatattca gcgccgcgta ttgcagcagc atgatggtct
     7081 tggcatcgac gatgcggccg tcgccgatca tggccagcgc agaatcgatc ggcaattcca
     7141 gcacctcgat gtcctcgccc tccgaggcga tgccgccgcc gcttccgacc ctcatacccg
     7201 cctcgtattc cgcgacaaag aaatgcagtt tctcggtgat cgcgccgggg ctcatgaagg
     7261 cttcgaatac cttttgcacc ttgccgagcc ggtagccggt ttcttcctcg gcctcggcga
     7321 gaattcgcac ttccggagat tcgttgtcca atagtcccgc cgcggcctcg atcaagagat
     7381 cgtcatagcc gttgacgaaa gccggatagc gaaactgccg caccagcacc acgctgcgct
     7441 gcgccaggtt gtagggcaac agtgccgcgc cgttgccgcg gtcgtaggtc tcgcgggctt
     7501 gcgtttgcca ttcgccgctg ttgcgccgcc actcgaaggt ggtggtcttc aggatgtaat
     7561 gattgtcgga gagcatccgg acatcattca cgcggacgcg gtcggaaacg gtcatgtgat
     7621 tcccattgat ttttgattac ggcgtcggct cgcggccgtc gagccacttg gcgtagatga
     7681 tgcgttcctg ctcgagataa ccgagcgatt gatagaatgc ctgaacctga ctgttgtcgg
     7741 gccgcaccat cagctgcagc ttttcaatgc cgcgcccgcg cagccagcct tcggcggcat
     7801 ccatgatgag gcggccatat cccttgtggc gacaatcggg gtcgaccgcg acgtaataga
     7861 cccagccgcg atgaccgtca tgtccgacca gcaccgaggc aacgatgccg ccgccgtcac
     7921 gcccggccag caccgccgcg ttcgccccgt tgcgggcgag cgcgatatcg gcggcgggat
     7981 cgttccacgg ccgcgtcaga ccgcagcgct gccacagcgc gattacatcg gcgatatctg
     8041 catccgcgat ctccgcgatc gcgagcgggg caggggctgt cgaagacacg cttacagcac
     8101 cttgccgggg ttcatgatgc ccgatgggtc gagcatggcc ttgatgccgc gcatcagccc
     8161 gatcgcgacc ttgtccttga cgtcggggag ttcgtcgcgc ttgagcacgc cgatgccgtg
     8221 ctcggccgag atcgacccac ccatccgcat cacgatcgcg aacaccaccg cattgacctc
     8281 gtgccaacgc gcgagaaaat ccgcaggctc ggcgccaacg ggctggctga cattgtaatg
     8341 gatattgccg tcgccgagat ggccgaacgg caccgggcgt gagcccggaa tcaatttgac
     8401 cacggcggcg ttggcctctt cgatgaaatc gggcaccgcg gcaaccggca ccgaaatatc
     8461 gtgcttgatc gagccgccct ccggcttttg cgcggcggac atctcgtcgc gcagcttcca
     8521 gaatgcagct cgctggctga ggttcgccgc gatcaccgcg tcatcgataa tgccctcgtc
     8581 gagtcccttg gccaggatcg attccagcgc ggcgcgggcg tcgtcgcgcg aggacgacag
     8641 ctccatcagc acgtaccagg gatgcttggc ttcgagggga tcgcggatgt cgatgccgtg
     8701 acgcacgctg aaatcgaccg cgatgtcgga aagcagctca aagctggtga ggctgccggc
     8761 ggcctcgttc tgcgatatcg acaatagcct cagcgcctgc gccggcgatg tcaggccgac
     8821 aaaggcggtt tccaccgcgc gcggccttgg aaacagtttt agggtcgccg cggtgatgat
     8881 gccgagcgtg ccctcggcgc cgatgaagag gttgcgcaga tcgtaaccgg tattgtcctt
     8941 tttcagtttt gacagcccat tcaggatgcg cccgtcggcc agcaccactt cgaggccaag
     9001 cgccatctcg cgcgccaccc cataggccag cgccgtggtg ccgccggcat tggtggagag
     9061 attgccgccg atggtgcagc ttccctccgc gcccagcgac agcggaaaca gccggtcgac
     9121 ctcggaggcg cgctgctgcg cgacctgcag caccacgccg gcctcgcagg tcatggtgtt
     9181 ggaggcggtg tcgatgtcgc ggatctcatt catccgcagc atcgagacca ccacctcgcc
     9241 gttatgcggg gtctggccgc cgacgaggcc agtgttgccg ccctgcggca ccagcgcgat
     9301 ccgatattcg gtggcaagct tgcagatggc ggagacttcg gcggtcgagc ccggccgcaa
     9361 caccagcggc gatcgaccgt gaaacaggtc gcgctcttcg gtgaggtagg gcgcgatgtc
     9421 ggccgcatcg gtgaccgcgt atttgtcgcc gacaattttg cggaaacgcg cgatcagttc
     9481 gggcgagagc ggggggcttg cgggctggag gatattcatc atcgtctttt gatggtctct
     9541 tacacgttta ctacttgcac gtttactcaa tcccgtccca tcgcggcgcg gcgcagccgg
     9601 tcgttgatcg cctcaccgag tccgtgatgc ggcaccggca tcaccgcgat agtatgcgcc
     9661 cccctggtgt cgagcgcgcg aagatagccg aaaagattgg cggcagcctc gtcgagatcg
     9721 ccgcgcgcgg acagattcat cacggaagtc gccgcgtcaa cacccgatac cgcaaccggg
     9781 ccaaacgcca gcagcgcctc gccgggctcg acgttacagg cattgagccg caccagcgta
     9841 cgcggcgcgt aatgcgatgc cagcatgccc ggcgccagag gccggccgct atcgctgtcg
     9901 gcgtcgccgg gcggctgcac cagcttacgg cccagcaggc gttcgatgtc gccgcgcggc
     9961 aggccgccgg ggcgcagcag gacggcttcg tcaaagcatc cgacaatggt ggattcgacg
    10021 ccgaccgcga ccggcccgcc atcgacgatc agatcgatcc gccccgcaag gtcgctctgc
    10081 acgtgcgccg ccgtggtcgg cgaaacgtgg cccgacagat tggccgatgg cgccaccacc
    10141 ggtccgccga acgcgcgcag gatgtcgcgc gctacccggt gcgccggcac ccggatcgcg
    10201 atggtatcga ggccggcggt ggcgaactcg gccacggcac agtcgcttgt cttcggcagc
    10261 accagcgtca gcggccccgg ccagaacgct tctgccagct tgatcgcagt ggtgtcgaag
    10321 cgggcaatcc gcttggccgc ctccagatcc ctgaccgtgg cgatcagcgg attgaacgcg
    10381 ggccggccct tggcctggta gagccgggcg atggctttgg ggttggtggc atcggcgccg
    10441 aggccataaa cggtctcggt cgggaacgcg accagcccac cgttcgccag cgaacgcgcc
    10501 gccagggcca ccgcggcctc gttagctgcc agaatctgcg ttgtcaggct cacattcact
    10561 gggtttggcg tcctcaattc ggcttcttgc gatatgcgaa acccaaggct ataagccgcg
    10621 ttcttgtcgg agtgtggctc agcccggtag agcactgcgt tcgggacgca ggggtcgcag
    10681 gttcaaatcc tgccactccg accaacatat cagatactta gcagattgca tcttcccggc
    10741 ccgggcattg gcgccatgtt tccgccccac gttttgcatg cagggtccgc cggttcaatg
    10801 gcataatgtt ccgggcagag ccgcaaggcg gccgttcgag gcccgttttc cggtagcggc
    10861 agcctgtccc cggatcctgc ggctgcggcg acagcccgcc gaccccagcg gtgcacctca
    10921 ttcggcggac gggatttagt gcagttgctt cgacaaagcg tgcggcatcg ttggcgccga
    10981 agatgcgcaa ttaaatgcaa caataaggcg atcacaatcc gtattggtgc cgatgctgat
    11041 gcgcagataa tcctctaact gcgagggcgc tctcggaaat gcggggatgc cgaacaaaca
    11101 gaaaattcgc agacgaagga acgacgtcga agccaaggtt tcgtccctac cgcgacatca
    11161 gaccagtcga tgtctgaggt ggactgagaa agacttcttg ctggccgccg cgttctcttc
    11221 ggaggcgcca tgagacgccg ggatttcact gactttcttc ggggcgcggc cgaagggggc
    11281 cgacgtgatt gatatgacct gccgacaggg ttggcacttg tagacgtgga tgagtgggtg
    11341 ggatccaagc cgagacagct cgccaacttt gacagtggcg cgcccgcagt tatcgcagga
    11401 cggcggacgc tctgcgtgca gttcggtcat acccaccaac ggcatttcca aatgaaactg
    11461 gttctctgtc aatggtaacc gagactgcgt cttaatcgtg gtgacaaagt ccgtaagact
    11521 acgaggtcgc ctagcccgct accgcttacg ctgcgccgca agagctcccg ccgatctgag
    11581 ccggcgtgac atcgctcaac gcgcttcatc acgaagcgga gcggtttcac gacgtcaatt
    11641 ccgggagact ttgaagtccg ctttgaatgc ctgggaggct gaacgttgcc ggcggaaatt
    11701 tgcgctcgcg gctatttgag tcatcgcgaa cggcgagggc gagcggaggc ctcgctgtac
    11761 ggacatcgcg atcgatcttc gaaagcctaa cggggttcgc taggggagcg cgtccggcca
    11821 gagcaatcgt aacccgcgcc gtcgaggtta ctcagccggg tcgaccgata cggcgcccac
    11881 aacagcagtt ggaagagtgc cctgccagcc tggtatttgg cagcgggtcg gcgccgttcc
    11941 gaaccctgca tgacaatctg cccttcggtc gcgcacacgc accacctcca ggcccggcct
    12001 cgcttcttca ggatcacttc aaaaatcgga aattggtcag gcattgcaat cgattcaata
    12061 gtcaagcgga gttccatatt tgtatctttc atctcgtcgc aggttcaaag tttccctgcc
    12121 agggagagaa aaattcaaca aaatcggcaa tgcctgtata gaagctacgt cctagccgag
    12181 cgctgtcgcg catccaacgc cggtaccgtg aagcgatgac cgaatcttcc aaacgatttg
    12241 aaactcaaaa catttggatg tcatcctcct ccttcaaatg tgggccgggt ccttggagat
    12301 cctggtgtcg ccgaaagact gcgagtgagc ggaagcacca ctttaaacgc cgccacgcac
    12361 gacatcctct gcccaatggc gtgtgaggat acggcggctt gagcagtatc gactataaaa
    12421 tagttccgaa ttgattgcgc ggaggctaca ctaaaagcga gtggccgctt ggagggtgta
    12481 acatgaaagc actcagtctt gggttgttat tggctatcac cacgttatcg agtgtacacg
    12541 cgcaacgctc tcctgaatcg ctgcctgctg tgacggttga ttgctaccaa tcgtttgccg
    12601 caaaggcgcc attgccagaa ctttcttcca aggaaatggc aatcgacggc ggtagtttcg
    12661 aaacgccgtt gatacgctac agactccaag tcgctgataa gacaactctt aaaataatca
    12721 aagatccaac tcgtcctgcg tttcgaactg aatacggcaa aagcgtttgg gagataaaac
    12781 gggattttac gaacaggcat cagattgtgg gttggcgaga agaattgccg ggtggaatcg
    12841 tcaggctctt cacattcgac tttgaggatc tcttgcttag tacaattgac gtatcaccag
    12901 cgagttcgtc cgccgccggg gtcgagcttc atgtgatgaa atgcagtaaa tcgatttagc
    12961 tcaactgcga ttgcgcggct atgctgtggc tctcacgcaa ggagtgctac tatgcctcgt
    13021 tcaaagcctg cgcgatgtcc gcgatcagat cggacgggtg ttcgatgccg atcgacagcc
    13081 ggatcgtgga gtcgagaacg cctatttttt gacggatgtc agccggaact ccggagtggg
    13141 tcatgcttgc gggcaggctg gcaagcgact cggtgccgcc caggctcacc gccagcttga
    13201 agatttgcag cgcgttcaag aatttgaatg cggcggcctg gccgccgaca atgtcgaacg
    13261 agaacgtgga gcccgcgccg gtgcattgcc tcgcaaacag acggccggcc ggggaggctt
    13321 cttcgtggtg agcaaggtaa tggacatcag ctactttggc gtggtcgcgc aagtaatccg
    13381 ccacgagccg cgcgtttcgg tcagcctttt ccatgcggat gctcaaggtc tcgagcgacc
    13441 ggctgatcat ccagcaggaa tgggggtcca gttgggtgcc aatggcgcct cgcaacgctt
    13501 tgacgtcttt catgagggct cttgagccga gcgcagcgcc cgcgatcagg tcggaatgac
    13561 cgccgatata tttggtcagc gagtacagcg aaagatcggc gccgtgttcg atcggccgct
    13621 gaaacaccgg cccgagcagc gtattgtcgc acgcaatgat tggcgtgtgt ccctgagcct
    13681 ggccgatcat ctcggcgacg cggcggacca tcgcgatatc gaccaggccg ttggtgggat
    13741 ttgccggggt ttcgatgaaa atcatcgcaa cccggcctct gcgcacggcg tgctccgccg
    13801 cccgcctgac tccggcttcg tcgatgccgt cggcaaagcc caccgcgccg atggagaggc
    13861 ctgcaagcgt cctcgcaaac agagtttccg tcccgccata gagtggttga gagtgcacaa
    13921 tgacgtcgcc agggcggacg aacgccagga tcgtggtcgc aatcgccgcc atgccggacg
    13981 agaacagtgc gcatttctcg gtgcgctcgt agacagagag cctgtcctcg acgatctcgc
    14041 tgttgggatg attgaaccgc gaataaacca gacccgcgcc catcccctcg ggaggctcgc
    14101 gccgacccga gacgaaatcg aagaagtcct gtccgtcttc ggcggtcctg aagacaaagg
    14161 tcgaggtcag aaagaccggt ggtttgacag ctccttccga cagttgcgga tcgtagccgt
    14221 agttcagcat caacgtttcg ggatgcagca tgtggttgcc gatgtgggtc ttcgacggaa
    14281 aaggtttgac catggcctgc ctcgctgtct ggattcggca cgtcgcctgc gttggggtct
    14341 tccgcaatga cgacgcggtc agcagcggtg gatgcgacgg tatccaactg ctccagtcag
    14401 ggttgccagg atcagtgtag caacatccgg aactgatttc accgcgcccg aggcgaattt
    14461 cgcagcgcgc atcgcggctg gcgcatatcc tggcagatcg cgcccgcgag cacttgcagg
    14521 ctgcaaggcg cgcccgctat agaacggtta tcgatagggg tcacatctca tggcactcat
    14581 caagaaggtt gaaactggtt tctatcgcat tccgctgtcc gtgacgctga ccgacagcac
    14641 gcatggagag atgaaggcat ttgagctgat cactatccgg cttcgtgatt ccgagggcgc
    14701 agaaggcgtc ggatatacct atacggttgg ccgaaacggg agagccgtag cggatcttct
    14761 ccgccgggaa attcccgaat tgatcgagga tcaagaagcc gacgacaccg aagcgatatg
    14821 gcagcgcatc tggtgggcgc tgcattacgg cggacgtggc gggccttcag tgttggcact
    14881 ctcggccctc gacatagcgc tgtgggattt gaaggcgcgg cgcgccaatt tgccactctg
    14941 gcgcctattg ggcggattcg atcggagcgt accttgttat gccggtggca tcgatctcga
    15001 tctctctatc gaagatttgc tgaagcaaac ggatgacaac ctcggcaagg gctttcgtgc
    15061 catcaagatg aaggttggcc gcccggatct ggcatccgat gtagcccgtg tcagggccat
    15121 gcgcgatcat ctcagcgggg gctttccgct gatggcggac gccaatatga agtggacggt
    15181 cgaagcagcg atcagagccg ccaaggcgtt ccagcccttt gacctgacct ggctcgaaga
    15241 gccgaccatc ccggatgatg ttcccggcca tcggcgcata ctggctcagg gtggagtgcc
    15301 cattgcggca ggagagaatc tccgctcgct ttgggaattc aagagctaca tcgtgagcga
    15361 tgccgtgtct tatcctgaac ccgacgttac caactgcggc ggggtgacct cgttcatgaa
    15421 gatagcgcgc ttggcagagt cgtttaatct tccggtgact agccacggcg ctcatgacat
    15481 caccgtgcac ctgcttgcag catgtccgaa tcgctcttac ctggaggcgc acggatttgg
    15541 gctcgatcga tacatcgaac atccgctgat cttggaggag ggcatggcga tcgcgccgat
    15601 gcgtccgggt cacgggataa gcttcgattg gaacgctctt gttccgctct ccgcctaaag
    15661 cgtcgcggag ggcagtgtga gacaagcaac tctttggaga tgcgatctca agcgcaattg
    15721 gcggaggcga cggccggcgg aatcaatcgc ggtccggcaa tgacgttcgg ctacattgcc
    15781 ggccggcacg ccgcgggcct caccggatac gaacaggccc gcggggatcg tcgggccgga
    15841 ggttcacgca tgaccagctt ctccctcgcc cacctgaccg tgctgaacgt gccgccgccg
    15901 gagatgatcc gggtggcggc gcgcaccggt tacgactgcg tcgggctgcg catgatcgcc
    15961 gtcacggaca ccacgccggg ctatccgctg atgcacgagc cggcgatgat gcgagccacc
    16021 aaggcagccc tcgccgacac cggggtgcgg gtgctcgaca tcgagtttgt caagatcacc
    16081 ccggagatcg acgtggccgg cctggagcgg ttcgtggcgg ccggcgcgga actcggagcc
    16141 aagcacgtga tcaccgctcc ttacgactcc gagcttgcgc ggttggcaga ccgactcggc
    16201 gccatcaccg acctgtgcgc ccgatacggc ctgcgcaccg tactggagtt cttcccatgg
    16261 accgttgtgc cggacctgga agcggcggtg cggatggtgg aggcggcagg gcggaccgag
    16321 accggcatcc tcgtcgatac gctgcatttc gaccgctccg gcagcagggt cgaacagctc
    16381 gaccgcattt cgcccgcacg actgcccttc gtgcacgtct gcgacgcgcc ggtgcaggac
    16441 acctacacga cggaagagct gcttcacgca ggccgggccg agcggctgcc ccctggtgag
    16501 ggcggcatcg acattggggg catcctgcgt cacatgccgc agggcatacc ggttgcgctg
    16561 gaggtgccga tgacggcgat gaacgctgcc gagggtgccg aagccgtcgc gctccgggtg
    16621 cgtcaagcgg cagagcgctt actggcggcc tgactggtcg gacgcactat ccggtctcgg
    16681 tacggttatc ggacgggcct ctcttgtttg aactgcgagc cgaaccgagg cattgggcgc
    16741 gccaaagccc tggtaaccgc gtcgctgcac gatttcgaag aagaaacgct catcaaacgc
    16801 gtgcgtgtag acttgaaaga attcaccagt ctcatcgcga tcatacaaaa tctggttttc
    16861 tctcatgcgt gtcgcgacgg cgggatcgag ctcaaacctg ctttcaacgt cgtcataata
    16921 attgtcaggg attttcagaa aagatgctcc cgcggagcgc atccctgcaa ccgtctcgaa
    16981 aatgtcgtcg cattcgaaag cgatgtgttg cactcccgcg ccaaaaaact ccgtcaggaa
    17041 gcgcgcagac atggtccgtg tcgccgcaga tccattaaga acgatacgga atctcttgtc
    17101 agggttcaga attgcttggc tctgtacgag cccgagaggg tccgcaattt caagctgcgg
    17161 tgacctttga aggtgaaaaa tgccggtgta gaattgttgc caggac
//
LOCUS       DATNOI010000148         6064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_303833,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000148 DATNOI010000000
VERSION     DATNOI010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303833"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /gene="ugpC"
                     /locus_tag="VN968_05640"
     CDS             complement(<1..836)
                     /gene="ugpC"
                     /locus_tag="VN968_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HXL28756.1"
                     /translation="MANVTLSDVRKTYPSGFEAIKGISMAVGDGQFCVLVGPSGCGKS
                     TLLRMVAGLETITGGEIDIGGRVVNQIEPADRDIAMVFQNYALYPHMSVFNNMAYGLR
                     NRGMAEPEIKTRVGEAARILELAAMLERKPRQLSGGQRQRVAMGRAIVRQPKVFLCDE
                     PLSNLDAKLRIAMRVEIRKLQRRLSTTSIYVTHDQLEAMTLADILVVMNGGQVEQIGN
                     PLDIYQKPATTFVASFIGAPPMNLMPLRSDEIKAQLADDNRTSEAGILGIRPEDFVIS
                     ND"
     gene            complement(999..1847)
                     /gene="ugpE"
                     /locus_tag="VN968_05645"
     CDS             complement(999..1847)
                     /gene="ugpE"
                     /locus_tag="VN968_05645"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018480638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HXL28757.1"
                     /translation="MVEHRRFGNLLPHLVLLAGVAIVAFPVYLAIIASTHDNAVIANG
                     QMPLIPGSHTIETYWNTIVSGTGRTTRQPVGGMMLNSLIMALGIAAGKIAISIISAYA
                     IVFFSFPFRMTAFWTIFITLMLPVEVRIFPTYKITSDLHMLDSYAGLILPLIASATAT
                     LLFRQFFMTVPKELVEASKIDGAGPMRFFWDTLLPLSVTNIAALFVILFIYGWNQYLW
                     PLLITTRDDMQTIVIGIKKIIDVHDALTEWQVAMATAVLAMLPPVAVVVLMQRLFVKG
                     LIETEK"
     gene            complement(1853..2734)
                     /gene="ugpA"
                     /locus_tag="VN968_05650"
     CDS             complement(1853..2734)
                     /gene="ugpA"
                     /locus_tag="VN968_05650"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HXL28758.1"
                     /translation="METRAIFSGKLLPYLLILPQLAVSLIFFYWPAVQALQQSFLVQD
                     AFGLSTEIVWFENYVSLLQTPEYYHAIGVTFVFSALVAFFSLTLGLLLAVMANRNIRG
                     VQIYRTFLIVPYAVAPAVASVLFIFMFQPGLGMIARAMQRNGIDWNPVLNGTHAMILV
                     VIVAVWNQISYNFLFFLAGLQAIPKSVIEAAAIDGARPMRRFWTITFPLLSPTTFFLI
                     IVNITYVFFNTLGIIDTATGGGPNGATQTLVYKVFQDGKVGTDLGGSAAQSVILMVIV
                     VALTAFQFRYVEKKVHY"
     gene            complement(2794..4107)
                     /gene="ugpB"
                     /locus_tag="VN968_05655"
     CDS             complement(2794..4107)
                     /gene="ugpB"
                     /locus_tag="VN968_05655"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HXL28759.1"
                     /translation="MNFRHYGIAATLASTLALATPAHAATEIQWWHAMTGPNNDVVVK
                     LANDFNASQGDYKIIPTFKGSYADTLNAGIAAFRAGNAPGIMQVFEVGTATMMAAKGA
                     VKPVSGLMKEQGESFDPKSYLPAITGYYSTSKGEMLSFPFNSSSMVLWINLDALKKAE
                     IAVPPKTWPEVFAAAKKLHATNPTCGFSSSWITWGLIEQFSAWHNVPIGSKANGLDGF
                     DTVLEFNTPLETKLLENLVELQKDKSYDYSGRTNTGEGRFTSGECPMYMTSSAFYPNV
                     KANAKFDYTAVAMPYFPDVKGAPQNSIIGGASLWVMGGKTPEEYKGIAKFFTFLSDTD
                     RQIYLHQVSGYLPITKAAYEKTKASGFYEKNPILEVPLKELTNSPPTENSRGLRFGGM
                     VQMRDVWAEEIEAALAGKKSAKDALDAAASRGNQMLRQFERSAVK"
     gene            complement(4316..5521)
                     /locus_tag="VN968_05660"
     CDS             complement(4316..5521)
                     /locus_tag="VN968_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXL28760.1"
                     /translation="MSSSAAPGAMTGLRVIDLTRVLGGPYCTQILADHGADVIKVEPP
                     AGDEVRDWGPPFHGEDAAYFVGINRNKRSIGLDLASEGGRAVLLKMLESADVLIENFK
                     PGTLDKWGIGSNVLRAKFPKLVHCRISGFGADGPRGGNPGYDAIIQAMTGMIAATGSP
                     QSGPMRIGVPLVDITTGLYAAIGILMALSERQRSGVGQFLETTLYETGLAIMHPHAAN
                     YFMHGKPPQLTGNEHPNLVPYAIFPTRTDNIFIGVGNDGTFRKLAKEIGKPELGSDMR
                     FARNKDRIANRDALRAELAAVFSQHDAAPLCDRLLAAGLPAGPVQSIDQALTSAHTVH
                     RGDVIEKDWYKGVASPIRLERSKPSLRNVPPKFSQHTTEVLTEFGYSTSEIDGLIAKG
                     VVCGTERKR"
     gene            complement(5705..>6064)
                     /locus_tag="VN968_05665"
     CDS             complement(5705..>6064)
                     /locus_tag="VN968_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HXL28761.1"
                     /translation="TLDGTLNVKLATPKELGGGGGAGNNPEQLFAAGYAACFIGAMKF
                     VASQGGPKVPADASVTSTVGIGPRSAGGFGLDIELDISLPGVPRADAEALVEKAHQVC
                     PYSNATRGNVDVRLKIV"
BASE COUNT         1162 a         1769 c         1955 g         1178 t
ORIGIN      
        1 ctgtcgttgg aaatcacaaa atcctcgggc cggatcccca gaatgccggc ctcgcttgtc
       61 cggttgtcgt cggccagttg cgccttgatt tcgtcggaac gcagcggcat cagattcatc
      121 ggcggagcgc cgatgaagga ggcgacgaag gtggtcgccg gcttctggta gatatcgagc
      181 ggattgccga tctgctcgac ctggccgccg ttcatgacca cgagaatgtc ggccagcgtc
      241 atcgcctcga gctggtcatg ggtgacgtag atcgaggtcg tggacagccg gcgttgcagt
      301 ttgcggattt cgacccgcat cgcaatgcgc agtttggcat cgaggttcga caagggctcg
      361 tcgcacagga acacttttgg ctgccgcacg atggcgcgtc ccatcgcaac gcgctggcgc
      421 tggccgccgg acaattgccg cggcttgcgc tcgagcatcg cggcgagttc gaggatccgc
      481 gcggcctccc cgacgcgggt cttgatctcg ggctcggcca tgccgcggtt gcgcaggcca
      541 taggccatgt tgttgaagac gctcatatgc gggtagagcg cgtagttctg gaacaccatt
      601 gcgatgtcgc gatcggccgg ctcgatctgg ttgacgacgc gcccgccgat gtcgatctcg
      661 ccgccggtga tggtctccag ccccgcgacc atgcgcagca gcgtcgactt gccgcagccg
      721 gagggaccga ccagcacgca gaactggccg tcgccaaccg ccatgctgat gcccttgatc
      781 gcctcaaaac cgcttggata ggttttgcgg acgtcgctga gggtgacgtt agccatggag
      841 ttctccggcg aatagcgaat agaagcgggc gaatagggag tagcggatgg cgaataagga
      901 aggacaacgg gcggaggggc gaacgttggc tccattcacc actcgccctt cgctattcgc
      961 cctgaccatt cgccattcgc tactcgccct tcgcatcttc acttctctgt ctcgatcagg
     1021 cccttgacga agaggcgttg catcaacacg acgaccgcga ccggaggcag catggcgagc
     1081 acggcggtcg ccatcgccac ctgccattcg gtcaaggcgt cgtgtacgtc aatgatcttc
     1141 ttgatgccga tgacgatggt ctgcatgtcg tcgcgcgtcg tgatcagtag cggccacagg
     1201 tactggttcc agccgtagat gaagagaatg acaaacaacg cggcgatgtt ggtcaccgaa
     1261 agcggcagca gggtatccca aaaaaaccgc atcggaccgg cgccgtcgat tttcgaggct
     1321 tcgacaagtt ccttcggcac ggtcatgaag aattgccgga acaacagcgt cgcggtggcc
     1381 gacgcgatca gcggcaggat cagcccggcg taggaatcca gcatgtgcag atcgctggtg
     1441 atcttgtagg tcggaaaaat gcgcacctcg accggcagca tcagcgtgat gaagatggtc
     1501 cagaacgccg tcattctgaa cgggaacgaa aagaacacga tcgcataggc ggagatgatc
     1561 gagatcgcga tttttcccgc ggcgatgccg agcgccatga tcaggctgtt cagcatcatg
     1621 ccgccgaccg gctggcgcgt ggtcctgccg gtgccggaga cgatggtgtt ccagtaagtc
     1681 tcgatcgtat gcgagccggg gatgagcggc atctggccgt tggcgatcac cgcgttgtcg
     1741 tgggtcgatg cgatgatggc cagatagacc ggaaaggcga cgatcgcgac ccctgccaga
     1801 aggacgaggt ggggtagcag gtttccaaat cggcgatgtt cgaccatggc gctcagtaat
     1861 gaactttctt ttcaacgtaa cgaaattgga acgcggtgag ggcgaccacg atcaccatca
     1921 ggatcaccga ttgcgcggcc gagccgccga gatcggtgcc gaccttgccg tcctgaaaca
     1981 ccttatagac gagggtttgc gtggcgccgt tggggccgcc gccggtcgcg gtgtcgatga
     2041 tgccgagcgt gttgaagaag acataggtga tgttgacgat gatcaggaag aacgtcgtcg
     2101 gcgagagcaa gggaaaggtg atggtccaga accgccgcat cggcctcgcg ccgtcgatcg
     2161 ccgcggcttc gatcacgctt ttcggaatcg cctgcaaacc ggcgagaaag aacagaaaat
     2221 tgtagctgat ctggttccag acggcgacga tgacgacgag gatcatcgca tgcgtgccgt
     2281 tgagcaccgg attccagtcg ataccgttgc gttgcatcgc gcgcgcgatc atgccgagcc
     2341 cgggctggaa catgaagatg aagagcacgc tggcgaccgc cggcgctacc gcatagggca
     2401 cgatgagaaa cgtgcgatag atctggacgc ccctgatgtt gcggttcgcc atcaccgcga
     2461 gcaggaggcc gagcgtgagc gagaagaacg ccaccagcgc cgaaaagaca aaggtaacgc
     2521 cgatcgcatg gtagtactcg ggagtttgca gcagactgac atagttctcg aaccagacga
     2581 tctcggtcga gagcccgaag gcgtcctgca ccagaaacga ctgctgcagg gcctgcacgg
     2641 caggccaata gaaaaagatc agggaaaccg cgagctgcgg caggatcaga agatagggca
     2701 gcagcttgcc tgaaaaaatc gcccgggttt ccatgatgcc tcgcttgatg gatcgtggcc
     2761 gcgttgcgcc cggccacgat ccttaagctt ccgtcacttg acggcgctgc gctcgaactg
     2821 ccggagcatc tggttgccac gcgaggcggc ggcgtcgagc gcgtcctttg ccgacttctt
     2881 gccggcgagc gccgcctcga tctcctcggc ccagacgtcg cgcatctgca ccatgccgcc
     2941 gaagcgcagg ccgcgcgaat tttcggtcgg cgggctgttg gtcaattcct tcagcgggac
     3001 ttcgagaatc gggttctttt cgtagaagcc cgaggccttg gtcttctcgt aggcggcctt
     3061 ggtgatcggc agatatccgg aaacctggtg cagatagatt tggcggtcgg tgtccgacag
     3121 gaaggtgaag aattttgcaa ttcccttgta ttcctccggt gtcttgccgc ccatgaccca
     3181 cagcgaagcg ccgccgatga tggaattctg cggcgcgccc ttgacgtcgg ggaaatacgg
     3241 catcgcaacc gcggtgtagt cgaacttggc gttcgccttg acgttcggat aaaatgccga
     3301 ggacgtcatg tacatcggac actcacccga cgtaaaacgt ccttcgccgg tgttggtccg
     3361 ccccgaatag tcatagctct tgtctttctg cagctcgaca aggttttcca gcagttttgt
     3421 ctccagcggg gtgttgaatt cgagcacggt gtcgaaccca tccagcccgt tggccttgct
     3481 gccgatcggg acgttgtgcc aggcggaaaa ctgttcaatc agcccccagg taatccagga
     3541 cgaggagaag ccgcaggtcg ggttcgtcgc gtgcaatttt ttggcggcgg cgaacacctc
     3601 cggccaggtc ttcggcggga ccgcgatctc ggccttcttc agggcgtcga gattgatcca
     3661 gagcaccatg ctcgacgagt tgaacggaaa cgacagcatc tcgcccttcg aggtcgagta
     3721 gtatccggtg atcgccggca gataggattt tggatcgaag ctctcgcctt gttccttcat
     3781 cagtccggaa actggtttga cggcgccctt ggcggccatc atcgtcgccg tgccgacctc
     3841 gaagacctgc atgatgccgg gcgcattgcc ggcgcggaac gccgcgatcc cggcattgag
     3901 tgtgtcggcg taactgccct tgaaggtcgg gatgatcttg taatcgcctt gcgaagcgtt
     3961 gaaatcgttg gcgagcttga ccacgacgtc attgttcggg ccggtcatcg catgccacca
     4021 ctgaatctcg gtcgcggcgt gggcgggcgt tgcgagcgcg agtgtcgagg ccagcgtggc
     4081 ggcaatgccg tagtgtcgaa aattcatcaa aaatctcccg gttggaccgc catcatcatt
     4141 aggcgcgtaa cagcgccgga tgacgcgcaa atgaccgtat aagcgatggg atgctacggg
     4201 ggaagcgccg gcgtggggaa gcgctaaaat aggcggaggt gagaccctgt gtcccggacg
     4261 cggtgcggcg ttcttgcgcc gctccgcaga cccgggacca ttcagggcac aagaactatc
     4321 gctttcgttc tgtcccgcag accacaccct tggcgatcag gccatcgatc tcgctcgtcg
     4381 aatatccaaa ttccgtcagc acctcggttg tgtgctggct gaatttcggc ggcacgttgc
     4441 gcaggcttgg cttgctgcgt tccaggcgga tcggggacgc gacgcccttg taccagtcct
     4501 tttcgatgac gtcgccgcga tggacggtgt gggcgctggt cagcgcctgg tcgatcgact
     4561 gcaccggtcc ggccggcaat ccggcggcga gcaggcgatc gcatagcggc gcggcgtcgt
     4621 gctgactgaa caccgcggca agctcggcgc gcagcgcgtc gcggttggcg atgcggtcct
     4681 tgttgcgcgc aaaacgcata tcgctgccga gttcgggctt gccgatttcc ttggctagtt
     4741 tgcggaaggt gccgtcattg ccgacgccga tgaagatatt atcggtgcgg gtcgggaaaa
     4801 tcgcgtaagg cacgaggttc ggatgctcat tgccggtgag ctgcggcggc ttgccgtgca
     4861 tgaaataatt cgcggcgtgc ggatgcatga tggcaaggcc ggtttcgtac agtgtggttt
     4921 cgagaaactg tccgacgccg gatctttgcc gctccgacag cgccatcagg atgccgatcg
     4981 ccgcataaag cccggtggtg atatcgacca agggaacgcc gatccgcatc gggcctgatt
     5041 gcggcgagcc ggtcgcggcg atcatcccgg tcatcgcctg aatgatggcg tcatagcccg
     5101 gattgccgcc gcgcgggccg tcggcgccga agcccgaaat ccggcagtgg accagctttg
     5161 gaaattttgc gcgcagtacg ttgctgccga tgccccattt gtcgagcgtg cccggcttga
     5221 aattttcgat caggacgtcg gcgctctcca gcatcttcag cagcaccgcg cgtccgccct
     5281 cggaggcgag gtcgagcccg attgagcgct tgttgcggtt gatgccgacg aaataggccg
     5341 cgtcctcgcc gtggaacggc ggtccccagt cccgcacctc gtcaccggcg ggcggctcga
     5401 ccttgatcac gtcggcgcca tgatcggcga ggatttgcgt gcaataaggg ccgccgagaa
     5461 cccgcgtgag atcgatcacg cgcaatccgg tcatcgcgcc tggggccgct gaggaactca
     5521 tggaagcaac cttggctcaa aaaaggggat cgattagggg agcgttcgat ttccagagct
     5581 agcgatgttc gggcgcggca gcaatgccta ctcgcgcgca ggcgcatgtc aggacagccg
     5641 acgagatgcg cctcgccttt gcgcgccgaa tggcccgccg cgtgtcgcag cgggccagcc
     5701 gtcattacac aatcttcagg cggacatcca cgttgccgcg ggtggcgttg gagtaggggc
     5761 atacctgatg ggccttctcc accagagcct ctgcgtccgc gcgcgggacc ccggggaggg
     5821 agatgtcgag ttcgatatca agcccaaagc caccggccga gcgcggtccg atgcctaccg
     5881 tggaggtcac cgacgcatcg gcgggcactt tcggtccgcc ttgcgaagca acgaacttca
     5941 tcgcgccaat gaagcaggcg gcatatccgg cggcgaaaag ctgttcggga ttgttgcctg
     6001 cgccgccgcc accacccagt tccttcggcg tggcgagttt gacgttcagc gttccatcga
     6061 gcgt
//
LOCUS       DATNOI010000149        15708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1875580,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000149 DATNOI010000000
VERSION     DATNOI010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15708
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875580"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="VN968_05670"
     CDS             <1..169
                     /locus_tag="VN968_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HXL28762.1"
                     /translation="IEQGSLYPALPRIREVSAHIAAAVAGVAYERGLATGQPPNDLVG
                     YIQLQMYDPHY"
     gene            complement(256..447)
                     /locus_tag="VN968_05675"
                     /pseudo
     CDS             complement(256..447)
                     /locus_tag="VN968_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase"
     gene            complement(458..1399)
                     /locus_tag="VN968_05680"
     CDS             complement(458..1399)
                     /locus_tag="VN968_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL28763.1"
                     /translation="MLDRLTGLEVFAKVAAAGSLSSAARAVGMSQTMVTKHIAALETR
                     LGVKLLHRTTRRLSITEAGRSYLEASERILAEFEAANAAVAADRVEPRGLLRLNAPVS
                     FGTRQIAPLLAEFAERHPLVTVELGLNDRLIDLAEEGWDLAIRIGNLSDSSLIARRIA
                     PCRMVVCAAPSYLEARGDPRTVSSLADHNCLGYTLSRLSGVDRWMFGARADVSIQVSG
                     DLRANNGDALRAAAIAGQGLIYQPTFIVADDLRAGTLVALSMDQPTVQFSGIYAVFPP
                     DRHPVAKVRTFIDFIATRFTPEPPWDRDLASPQKLTP"
     gene            1499..1939
                     /locus_tag="VN968_05685"
     CDS             1499..1939
                     /locus_tag="VN968_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXL28764.1"
                     /translation="MIDSRTAPYAALSLRVSLGMLFLAHASLKLFVFTPAGTAKFFGS
                     VGLPPEFAYVVITAEVFGGIALILGIWTRVVAIAATPILLGAIFTVHGAAGFFFTNAH
                     GGWEYPAFWTIALVVQALLGDGIYALRVPRARGFAGRLTDAHSH"
     gene            2003..2854
                     /locus_tag="VN968_05690"
     CDS             2003..2854
                     /locus_tag="VN968_05690"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HXL28765.1"
                     /translation="MTMPSLFLSHGAPTLPLTDAPARAFLNELGDLFARPKAILVISA
                     HWETSVPTVGAVDRNEIIHDFSGFPRALYELRYPAPGSPSVAARVSDLLRAAEFDCNI
                     DRRRGLDHGAWVPLLLMYPQADIPVLQLSVQPHLGPKHHLLVGRALAPLRKEGVLIIG
                     SGSLTHDLSEFRGHGPNDPAPDWVNGFADWFDCALTTGQTDDLLDYRRQAPFAIKNHP
                     SEEHLLPLYAALGAAGGNARAERLHASSTYGVLRMDVYAFQAPGDLIRNERNEVIGLK
                     DGIIQNN"
     gene            complement(2922..3455)
                     /locus_tag="VN968_05695"
     CDS             complement(2922..3455)
                     /locus_tag="VN968_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL28766.1"
                     /translation="MTSTASQPGAAIPPDDANRKLTVADPDDPKLRHVSVAGGTYTIL
                     VSGEQTGGRYCLIDMLVPAGGGPPPHRHDFEEMFTILDGEIELTFRGEVQRASTGSTV
                     NVPANAPHSFKNMSDKPARLLCMCTPAGQEEFFMAVGDPVDSRTAPPPQLSKEERAER
                     IQRAEALSAKYRTELLV"
     gene            3925..4722
                     /locus_tag="VN968_05700"
     CDS             3925..4722
                     /locus_tag="VN968_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HXL28767.1"
                     /translation="MKRAFGAAALGCLLAAAALAQDMMRDVDLSSADMVSAEMTRAEV
                     EAALAAATVSAPADFTRKRLSGLDLSGLDLSGAVFRAARLNKTKLVGAKLDLAVLDQA
                     WLLEADLTGASLKGANLFASQMRRARLDGADLSQARVAADLTGASLVGASIAGANLSA
                     DMRNQSMGLIRTVLKSANLERLNARGADLSRVDLEFASLKGADLTDASLKGALLAGTD
                     LTRVTFVGTDFDSADLASARLSAPVGLDRAKNFDKVKNRDRLIRIGE"
     gene            complement(4778..5998)
                     /locus_tag="VN968_05705"
     CDS             complement(4778..5998)
                     /locus_tag="VN968_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXL28768.1"
                     /translation="MNANARELAANFDIEKLTGEFYADPYPTYRALREHEPVKLLPNG
                     SRFLTRYDDLVSAYKNTKPFSSDKRKEFLPKYGDSLLYEHHTTSLVFNDPPAHTRVRR
                     LIMGALSPRAIAEMEPDLIALVDRLLETIAAKGSCELIGDFASAIPVQVIGNLLDVPM
                     EEREPLRDWSLAILGALEPVIGADVFARGNEAVKDFLSYLETLVAQRRTKPGNPERDV
                     LTRLIQGEDNGERLTEKELLHNCIFLLNAGHETTTNLIGNGLVALSENPDEKRRLIEN
                     PGLIKTAVEEILRFESSNQLGNRMTTAQVELGGITLAAGTPVTLCIGAANRDPAQFAK
                     PDNLDIGRSPNRHLAFGTGAHQCAGMALARLEGAIAISRFLARFPGYALNGPPVRGGR
                     VRFRGFLSVPCVVG"
     gene            6112..6657
                     /locus_tag="VN968_05710"
     CDS             6112..6657
                     /locus_tag="VN968_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HXL28769.1"
                     /translation="MHARTDAPPETAASWPEDIFEILQRFDVRQVPYVPDAGHSQLIE
                     RVLGASSMRAVPLTTEEEGVALLAGAWAGGQRGVLLMQSSGVGNCVNMLSLTQIFRFP
                     FLTLVTMRGEWGEFNPWQVPMGSSTAAVFELSGIKVLRASHAEEVREVVEAAAGQAYN
                     ACTPTAVLLSQRLIGAKVFTK"
     gene            6654..7244
                     /locus_tag="VN968_05715"
     CDS             6654..7244
                     /locus_tag="VN968_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXL28770.1"
                     /translation="MSQANLLERRSVVSHLLAERNDAIVVGGLGASTYDIAAAGDHDR
                     NFYLWGAMGGAVMIGLGMALAQPKLPVVVITGDGEMLMGMGSLATVGLQKPANLTIVV
                     LDNEVYGETGGQVSHTAATVDLVGVARSCGIPDARAIATMADIGTFASSMQDISSGPR
                     FASIKIDSANLERVLSSRDGQFTVTRIRGALGFGPI"
     gene            7314..8018
                     /locus_tag="VN968_05720"
     CDS             7314..8018
                     /locus_tag="VN968_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL28771.1"
                     /translation="MSTLRMTGDLRRQLILSAAKRCFARNGFAGTTTKSVAAAASISE
                     GLLFKHFPTKSALYAEILAEECEADPALHRLLGLEPSTGTLVILIREMVRHFQEVADA
                     PDQQEAQRLRLMITSQLDDGEFARLIYEKIGNLIGPVFTASLQQAVAAGEASRIAGDP
                     LNLFWFAHHTVLMATLARLPSVPCLPSGNAADLGRQICEFILRGIGLNELAVVSHLDR
                     ELSPDPGQQPLTAEGA"
     gene            8020..9213
                     /locus_tag="VN968_05725"
     CDS             8020..9213
                     /locus_tag="VN968_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXL28772.1"
                     /translation="MNIVTEANISGRAITEKPRARPVRMVRWFIIVGLLLALLVGGLV
                     WFNHFRGQMIAQFFANNKPPPSSVSIAEAKSEVIPNLLTAVGGLAAVHQVDVTSDVSG
                     RITDIMFTPGATVKAGNPLVQLFDAPDQADLANFKAQATVAQLSLDRAKQLASRQFGP
                     QATVDSAQAAYDQAQAGIAKTEAIISQKLVRAPFDGELGVRHVEVGQFLTAGTQIVTL
                     TDLSTLYANLTVTEKDSAALKVGQTVRILVDAYPGRTFEGKITAIEPQIAADTRNIRV
                     QATLENPDHILKPGMFATTTVVLPDKPAVVTVPETAVDYTLYGDSVFLITEKKADDGK
                     TSLTAVRTFVRSGNRIGGRAEILSGLKPGDRVVAVGQLKLQSGAAVAISTDAPPPIPA
                     NPPRY"
     gene            9238..12333
                     /locus_tag="VN968_05730"
     CDS             9238..12333
                     /locus_tag="VN968_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MexW/MexI family multidrug efflux RND
                     transporter permease subunit"
                     /protein_id="HXL28773.1"
                     /translation="MVWTDIFIKRPVLSVVVSLLILLIGFRAATTLPIRQYPKLSNTV
                     VNVTTVYPGASADLIGGFITTPIEQAVASAEGVDYMTSSSVQGTSTIQVYIKLNFDPN
                     QALTEVLAKVNSVKYLIPKESNDPIVTKTTGQTTAVMYLGFSSEVLSGSAISDYLTRV
                     VQPVLSTVDGVASADILGGQTFAMRVWLDPVRMAGRNVSPADVNAAIAANNFQAAAGQ
                     AKGYFIVSNVSTNTDLRNLDQFKRMIIKSKDGGFVRIEDIATVELAAQSTDASVAFNG
                     EHAIFIGVQATPQGNPLTLVKGVRALFPELERNLPPSMKMKVAYDSTKFIQSSIDEVE
                     KTLGEAVIIVVVVIFMFLASFRSVIIPVVTIPLSLIGVCSLMLIAGFSFNLLTLLAMV
                     LAIGLVVDDAIVVVENIHRHLEEGKPPVQAAMQGAREIVGPVVSMTITLAAVYAPIGF
                     LGGLTGSLFREFAFTLAGSVIVSGVIALTLSPMMCSIFLKNAEEGRFAKIINNVFGSV
                     TRWYGRRLDRSLDYRPITGVFALTILGLVGFLYLHTSKELAPEEDQGIVFSVIKGPKY
                     ANIDYLDFYGEKLDKAFSSFPETDLRFALNGINGPQNGIAGMLLKPWDERERSSIKLK
                     PLVQAQLSKIEGVNAFAFNLPPLPGGPGGLPVQMVINSTNGFQSVYEEMLKLKDAARK
                     SGLFIVSDSDLDFNQPVVRVKIDRSKASDLGITMQNVGASLATLLGGNYVNRFNLEGR
                     SYQVIPQVPRDWRLSPEALGGYYVPTATGQQVPLSTLVSIETATDPNALTHYNQLNSA
                     TFQAVPMPGVTVGQAVDFLEGAAKNLPAGFSHDYLADSRQYVQEGNQLAVTFGFALII
                     IFLVLAAQFESLRDPLVIMISVPMAIVGALIPLFFGMATMNIYTQVGLLTLVGLITKH
                     GILMVEFANELQLNEGLDKRSAIEMAARIRLRPILMTTAAMVTGLLPLLTATGAGAAS
                     RFSIGLVVVSGMSIGTLFTLFVLPAVYVAIATDHRAAAESERSKEIADFDLGPKALKP
                     T"
     gene            12425..>15708
                     /locus_tag="VN968_05735"
     CDS             12425..>15708
                     /locus_tag="VN968_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXL28774.1"
                     /translation="MSISLHPDTPEGRTLGGSPAATVAPEVGPSGTKIRSRLFTKYVA
                     LFVAVVGIALLSNGIFEVFFYYREHKAALIRIQREQAEAAAAKIGQFVKEIESQLGWT
                     TQLPWSAGSLENRRFDALRLLKQVPAITELAQLDSTGKERMRVSRLAMDVADSGLDLS
                     KDPKFTEAVAHKVYYGPVYFRRESEPYMTIALAGTRKDAGVSIAEVNLKLIWDVVSQI
                     KVGERGHAYVVGAEGRLIAHPDISLVLRNTDMSRLAQVQAALAALAAVLAAQAEQAAL
                     TAQAAQTGQAAPPPQVLPATPAWTANSGPDALQGALNIQGQEVLTASAPILPLGWIMF
                     VELPVKEAYASLYAALQRLATVLAAALIFAVLAGIFLARRMVGPIQALRAGAERIGGG
                     DFAQRISIRTGDELEGLANQFNDMGTRLQESYADLENKVETRTAELTESLEQQTATSE
                     VLEAISSSAGELDPVFRKMLENATRICGANFGQMNLYEEGSYRPVAHYNVPAAYAASL
                     AHTLFQPHPQSGLGTVARTHQVVHIEDIRALPPYLEGNPSVVALADFAGARTYFVVPM
                     LKENELIGAITIFRQQVKPFTEKQTELVANFAKQAVIAIENTRLLKELRQRTGDLTES
                     LEQQTATSEVLQVISSSPGELKPVFQKMLENAVRVCGANFGTMNLWDGDSFNIVADHN
                     VPPAFAALRQRMPIRPHPDTPMAAVVRTHQVFHAHDLKDNPAYLAGRSNVVNMVDIAG
                     ARTIVVVPMLKEDELIGVISIFRQEVRPFTDKQIALVENFTKQAVIAIENTRLLRELR
                     ERTDDLSESLQQQTATADVLKVISRSAFDLKSVLTTLAESAKSLCGAAFGVIFLRDGE
                     VLRDQAESGCPPALMNFLRDHPIRKSRATFTGRVFMDGKVAHLPDVLLDPEYNFGAAP
                     ELGGYRAALAVPLMRDGGVEGVLSLARPDPGPFTSRQIELVQTFADQAVIAIENARLF
                     GQVQERTRELSLSLDELRTAQDRLVQTEKLASLGQLTAGIAHEIKNPLNFVNNFSALS
                     AELIDELNDVLKPAALDGKMREEIDELTHMLKGNLEKVVQHGKRADSIVKNMLLHSRE
                     GSGEHRP"
BASE COUNT         2912 a         4858 c         4882 g         3056 t
ORIGIN      
        1 catcgagcag ggcagcctct atccggctct gccgcggatt cgagaggttt ccgctcacat
       61 cgccgcggcc gtcgccggcg tcgcttacga gcgcggcctg gcgaccggcc agccccctaa
      121 cgatctcgtc ggctacattc agttgcaaat gtacgacccg cattattgaa gcgatccgac
      181 ctggccttcg cacggcaacg gcgaagtaga actccggcgc gtcttagcct gggcgttgcc
      241 gcagggcttg tcggatgcca tctcgaccgg agaatagtcg ccatccaccc gatcgagccg
      301 aaacggttcg gccggatgaa gtggcggttg cgccatgaag cgcggcaccg agccccggtg
      361 catctgcgcg gcatgcacga gaaaaggatg gcacaagtag acgctgccag cttcgcccgt
      421 tgccaaagcc tctggtcgct cggccccatc ggtccgctca aggggtcaac ttttgaggtg
      481 aagcgaggtc acgatcccag ggcggctcgg gtgtaaagcg ggtcgcgata aagtcgatga
      541 acgtgcgcac tttggcgacc ggatggcgat ccggcgggaa cacggcataa ataccgctga
      601 actggacggt cggctgatcc atcgagagag caaccagcgt gcccgcgcgc aaatcatcgg
      661 cgacgatgaa cgtcggctgg tagatgagac cctgtcccgc gatcgcggcg gcccgcaggg
      721 catcgccatt gttggcgcgc agatcgcccg aaacctgaat gctgacgtca gcgcgagcgc
      781 cgaacatcca tcggtcgacg ccgctcagcc gggacagggt gtagccgaga caattatgat
      841 ccgccaggct cgaaaccgtg cgcggatcgc ctcgcgcctc gagataggag ggcgccgcac
      901 ataccaccat ccggcagggc gcaatgcgtc gcgcgatcag gcttgaatcg ctgaggttgc
      961 cgatccggat cgcaagatcc cagccctctt cggcaaggtc gatcaagcga tcgttaaggc
     1021 caagctcgac ggtgacgagt ggatggcgct ctgcgaattc ggcgagcaat ggggcgatct
     1081 gccgcgtgcc gaacgagacc ggagcattga gccggagcag cccccttggt tcgacccgat
     1141 ctgccgcgac agctgcgttg gcggcctcaa attcagcaag gatgcgctcg gaggcttcaa
     1201 ggtagctgcg cccggcctcg gtgatcgaaa gccgccgtgt ggtgcggtgc aagagcttga
     1261 cgcccagtcg cgtctccagc gcggcaatgt gcttggtgac catggtctgc gacatgccaa
     1321 cggcccgcgc tgcagacgac aggcttccgg cagcggcgac tttggcgaat acctcgaggc
     1381 ctgtcaggcg gtccaacatg gcttatctca ctctctgggt gtgatcgtat ttccaaattg
     1441 ccatattatc ccactcagaa gaataggcca tggatctccg accaatcagg agacagccat
     1501 gatcgattcc cgaaccgctc cctatgccgc gctgtcgctg cgcgtcagcc tgggcatgct
     1561 ctttctcgcg catgccagcc tcaaattgtt cgtcttcacg ccggccggaa ccgcaaagtt
     1621 tttcggcagc gtcggactcc cgcccgagtt cgcctatgtc gtcatcactg cggaagtgtt
     1681 tggcggcatc gcgctgatcc ttggaatctg gacccgcgtt gttgcgatcg cagccacgcc
     1741 gattttgctc ggcgccattt tcacggtgca cggtgcagcc gggttctttt tcaccaacgc
     1801 tcatggcggc tgggaatatc cggcgttctg gaccatcgcg ttggtcgtcc aggcgttgct
     1861 cggcgatggc atctatgcgc tgcgcgtgcc gcgtgcccgt ggtttcgccg gccggttgac
     1921 cgacgcgcac tcccattgaa cgaggcggcg gggccgtatt tcggccctgc atgatcagac
     1981 caagtgcatc ggacatcgtg tcatgaccat gccaagcctg ttcctgtcgc acggcgcgcc
     2041 gaccttgccg ctcaccgacg cgccagcccg cgcgtttctg aatgaactcg gcgacctgtt
     2101 cgcgcgtccc aaggccattc ttgtgatctc agcacattgg gagacatcgg taccgacggt
     2161 tggcgccgtg gatcggaacg agatcatcca cgacttcagc ggattcccgc gggcgctcta
     2221 tgagttgcgc tatccggctc ccggctcgcc gtccgttgcc gcgcgcgttt cagacctgct
     2281 gcgcgcggcc gaattcgact gcaacataga tcgtcgccgt ggcctggatc acggcgcctg
     2341 ggtgccgctg ttgctgatgt atccgcaagc cgacattccg gttctgcaac tctcggtcca
     2401 gccgcacctt ggaccaaaac atcatcttct cgtgggacgc gccctggcgc cgctgcggaa
     2461 agagggcgtg ttgatcatcg gcagcggcag cctgacgcac gatttgtcgg aatttcgcgg
     2521 ccatggtccg aacgatcccg cacccgattg ggtcaacggt tttgccgact ggtttgattg
     2581 cgcgctcacc acgggccaaa ccgatgatct gctcgactat cggcgacagg cgccgttcgc
     2641 gatcaagaac cacccaagcg aagagcacct tttgcccctg tacgcggcat tgggtgccgc
     2701 cggaggaaac gcccgcgccg agcggcttca cgcaagttcc acctacggcg ttctgcggat
     2761 ggacgtttat gcgtttcagg caccgggcga tttgatacga aacgagcgca acgaagtcat
     2821 tggtctgaag gacgggatca ttcaaaacaa ctgagcctgc gtgcccgcct cgcgcacgat
     2881 ctcgcgaccg gtgccaccgg tcgcgcccag aacgaggagc ttcagaccaa cagctcggtt
     2941 cgatatttgg ccgaaagcgc ctcggcgcgt tggatgcgtt cggcccgctc ttccttgctg
     3001 agctgcggcg gcggcgcggt gcggctgtcg acggggtcgc cgaccgccat gaagaactct
     3061 tcctgtcccg ctggcgtgca catgcagagc aggcgtgccg gcttgtccga catgttcttg
     3121 aacgagtggg gcgcattggc ggggacgttc acggtcgagc cggtgcttgc ccgctggacc
     3181 tcgccgcgga aggtcaactc gatctcgccg tccaggatcg taaacatctc ctcaaaatcg
     3241 tgacgatgcg gcggcgggcc gccgccggcc ggcaccagca tgtcgatcag gcagtagcgg
     3301 ccgccggtct gctcgccgct cacgaggatc gtgtaggtgc cgcccgcgac cgagacgtgg
     3361 cgcaacttcg gatcgtcggg atcggccacc gtgagttttc ggttcgcgtc atcaggcggg
     3421 atcgctgctc cgggctggct ggccgtgctg gtcatcggat gtttcctttc atggaatggc
     3481 agcgcacgtc acacccgtac acgccggccc ggtattgaac gcatcgggat tgagatggtt
     3541 cgtcacgcgg cagccgccac atgcggcagc tttctctggg catccagaat gcctagcgga
     3601 gttagccgcg gataaaatgc ccaagtcggt caatgacaaa tttttgatcg ctgataatac
     3661 ttttcaccag attcaccgat ccagaccaat ccaaggccct tttcctgctc aatgacactc
     3721 aagcgctttg acaggacagc agttcagacc aaccacattg atccagatca tacccaatcg
     3781 cgccaggccg catattttgc tagatctatc ggtccgtgac gaccaagacc gagatcgcgg
     3841 gaaagaactg gtgacgatcg cacccttggc cccataggcg gcgtctgcct tgcgtcgcaa
     3901 ggcacgtcgc tgtcggagtt ggcgatgaaa cgagcgttcg gagcggctgc actaggctgt
     3961 cttctcgccg cggcggcgct ggcgcaggac atgatgcgcg acgtcgacct gtcgtcggcc
     4021 gatatggtct ctgcggaaat gacgcgcgcg gaggtcgagg ccgcgcttgc tgcggcgact
     4081 gtctccgcgc ccgccgattt cacccgaaag agattgtccg gcctcgacct ctccgggctc
     4141 gatctgtccg gcgcagtttt tcgcgcggca cggctcaaca agacgaagct tgttggcgcg
     4201 aagctcgatc tagccgttct cgaccaggca tggctgctcg aagcggacct cacgggcgcc
     4261 agcctgaagg gcgcaaatct tttcgcctca cagatgcgtc gcgcccggct cgacggcgcc
     4321 gatctttcgc aagcgcgcgt ggcggctgac ttgaccggcg cgagcctggt cggagcttcg
     4381 atcgcgggcg caaacttgag tgccgacatg cgcaaccagt cgatgggcct catccgcaca
     4441 gtgttgaaat ccgcgaatct ggaacggctg aatgcgcgcg gcgccgacct gtcccgcgtt
     4501 gatctcgagt tcgcttcgct gaagggcgcc gacctgaccg acgcttccct caagggggcc
     4561 ctgctcgccg gcaccgacct gacccgcgtt accttcgtcg gcaccgattt cgacagcgcc
     4621 gatcttgctt cggcgaggct cagcgctccg gtcggtctcg acagggccaa aaatttcgac
     4681 aaggtcaaaa atcgcgaccg gctgataagg ataggagagt gacggctcca agggcccggt
     4741 cacaatcggt ggacggctgg cgtgaatcct ccgcacctta tccgaccacg cacggcacgc
     4801 tcaagaatcc gcgaaagcga acgcggccgc cgcgcaccgg cggtccgttc agcgcgtagc
     4861 cgggaaaccg cgccaggaat cgcgaaatcg caatcgcgcc ttcgagccgc gccagcgcca
     4921 tcccggcgca ttgatgcgcg ccggtgccga acgcaagatg ccggttgggg ctgcggccga
     4981 tgtcgagatt gtccggcttg gcaaattgcg cgggatcgcg gttggccgcg ccaatgcata
     5041 gcgtcaccgg cgtaccggct gccagtgtga tgccgccgag ctcgacttgc gccgtcgtca
     5101 tgcggttgcc gagctggttc gagctttcaa atcgcaagat ttcctcgact gcggtcttga
     5161 tcaggccggg attttcgatc agccgtcttt tctcatcggg attttccgat aacgccacca
     5221 gtccgttgcc gatcaggttg gtggtggtct cgtggccagc gttgagcagg aagatgcagt
     5281 tgtgcagcaa ctccttttcg gtcagacgct cgccattgtc ctcaccctga atgaggcgcg
     5341 tcagcacgtc gcgttcggga ttgccgggtt ttgttcggcg ctgcgccacc agggtttcca
     5401 gatagctaag aaaatccttc accgcttcgt tgccacgggc aaacacgtcc gcgccgatca
     5461 cgggttcaag cgcgcccagg atggccagcg accagtcgcg taacggttcg cgctcctcca
     5521 tgggaacatc gagcagattg ccgatcacct gaaccgggat cgcggaggca aaatccccga
     5581 tcagctcgca gctgcctttg gcggcgatgg tctcgagcag ccggtccacc agcgcgatca
     5641 gatcgggctc catctcggca atcgcgcgcg gcgacagcgc gcccatgatc agccgccgta
     5701 cgcgggtgtg cgcgggcgga tcgttgaaga caaggctggt ggtgtggtgt tcgtagagta
     5761 gcgaatcgcc atatttcggc aaaaactctt ttcgcttgtc ggaggagaaa ggcttggtgt
     5821 ttttgtaggc gctgacaagg tcgtcatagc gcgtcagaaa gcgggagccg ttgggcaaaa
     5881 gcttgacggg ctcgtgctcc cgcaaggcac gataggtcgg gtaggggtcg gcatagaatt
     5941 cgcccgtcag cttctcgatg tcgaaattgg ccgccagctc tcgcgcattc gcattcatgc
     6001 tgccatactc acaggtagag accgatatgt cgttttcgct ctttcgaaaa acgcggttgg
     6061 ctctatggtc ccgccccgga atacagcctc gccgaccgac tggaaatcaa aatgcacgct
     6121 cgcacggacg cgcctcccga aactgctgcc tcctggccgg aggatatatt cgagatcttg
     6181 cagcgcttcg atgtccgtca ggtgccttac gtgccggatg ccggtcattc gcagctgatc
     6241 gaacgcgtgc tgggcgcgtc ctcgatgcgc gcggtcccgt tgacgacgga agaggaaggc
     6301 gtggcgctgt tggccggcgc ctgggccggc ggccagcgcg gcgtgctgct gatgcagtcg
     6361 agcggggtcg gcaattgcgt gaacatgctg tcgctgaccc aaatcttccg ctttccgttc
     6421 ctgaccctgg tgacgatgcg cggcgaatgg ggcgaattca atccctggca ggtgccgatg
     6481 gggtcgagca cggcggccgt gttcgaactg tcgggaatca aggtgttgcg agcttctcac
     6541 gccgaagagg tgcgggaggt cgtcgaggcg gcggcggggc aggcctacaa tgcctgcacg
     6601 ccgaccgcgg ttctgctgtc gcagcgtctg atcggggcaa aggttttcac caaatgagcc
     6661 aagcaaatct ccttgagcgg cgcagcgtcg tctcgcacct gcttgcggag cgcaacgacg
     6721 ccatcgtggt cggcggattg ggcgcatcga cctacgatat cgccgccgcc ggcgatcatg
     6781 accgcaactt ttatctctgg ggcgccatgg ggggcgcggt catgatcggg ctgggcatgg
     6841 cgctggcgca acccaaactg ccggtggtgg tgattacggg cgacggcgag atgctgatgg
     6901 ggatgggcag tcttgcgacc gttggcctgc agaagcctgc aaatcttacc atcgtggtgc
     6961 tggataacga ggtttatggc gagacgggcg gacaggtgag ccacaccgcg gccaccgtcg
     7021 acctcgtcgg cgtcgcccgg tcttgtggga tcccagacgc ccgcgcgatc gccaccatgg
     7081 ctgatattgg aacctttgcg tcgtcgatgc aggacatctc gagcgggcct cgctttgcca
     7141 gtatcaagat tgacagcgcc aacctggagc gcgtgctttc cagccgggac ggtcaattca
     7201 ccgtgacgcg catccgtggg gcgctcggat ttggcccgat ttagcccgga ttgaggctag
     7261 tgcactgcag cagtttcctt gacatttgtt aaggtgagtg cttactcacg agcatgagca
     7321 ccctgcgcat gaccggcgac ctgcggcggc aattgatcct gagcgccgcc aagcggtgtt
     7381 ttgcccgcaa cggctttgcg ggaaccacaa ccaaaagcgt tgccgccgct gcctcaattt
     7441 cggagggatt gctgttcaag cattttccga cgaaatccgc gttgtacgcg gagatccttg
     7501 cggaagaatg cgaggcggat ccggcgctgc accggttgct ggggttggag ccgtcgacgg
     7561 gaacgcttgt cattttaatt cgcgaaatgg tccgccattt ccaggaggtc gccgatgctc
     7621 ccgaccaaca ggaggcccag cgtctcaggc tgatgatcac cagccaactc gacgacgggg
     7681 aattcgcccg gctgatttac gagaagatcg ggaatctgat cgggccggtg ttcaccgcat
     7741 cgctgcagca ggccgtggcg gcaggggagg catcccggat cgcaggcgat ccgctcaacc
     7801 tgttctggtt tgcgcatcac accgtgctga tggcgacgct ggcgcggctg ccatcggttc
     7861 catgcctgcc ctccggcaac gctgccgatc tcgggcggca gatttgcgaa ttcattctcc
     7921 gcggaattgg acttaacgaa ctcgctgtcg tctctcacct ggatcgcgaa ttgtcgccgg
     7981 atccggggca gcagccgcta actgcagaag gtgcatgaca tgaacattgt aaccgaagcc
     8041 aatatctcgg gcagggcgat cacggagaag ccgcgcgcgc gcccggtccg gatggtgcgc
     8101 tggttcatca tcgtcgggct gctgctggcg ctcctggtgg gcggtctcgt ctggttcaat
     8161 cacttccgcg gccagatgat cgcgcagttc ttcgccaaca acaagccgcc gccgtcgagc
     8221 gtcagcatcg cggaggcgaa atccgaagtg ataccgaacc tgctgacggc ggtgggcggt
     8281 ctcgccgccg tgcatcaggt cgatgtcacg tccgacgtct caggccgcat caccgacatc
     8341 atgttcaccc ccggcgcgac ggtgaaggcc ggaaatccgc tggtgcaatt gttcgacgct
     8401 cccgaccagg ccgaccttgc gaacttcaag gcgcaggcga cggtggcaca gctgtcgctc
     8461 gatcgcgcca agcagctggc ctcgcgccag ttcggcccgc aggccacggt ggattcggcg
     8521 caggccgcgt atgaccaggc ccaggcgggc atcgccaaga ccgaggcgat catttcgcag
     8581 aagctggtgc gcgccccgtt cgacggcgaa ctcggcgttc gccacgtcga ggtcggccag
     8641 ttcctcaccg ccggcaccca gatcgtgacg ctgaccgatc tgtcgacgct gtacgccaat
     8701 ctcacggtga cggagaagga cagcgcggca ctcaaggtcg gacagaccgt gcggattctg
     8761 gtcgacgcct atcccggccg tacctttgag ggaaagatca ccgcgatcga accccagatc
     8821 gccgccgata cccgcaacat ccgcgttcag gccacgctcg agaatcccga tcatatcttg
     8881 aagcccggca tgttcgcgac gacgacggtg gtgcttcccg acaagccggc ggtcgtcacc
     8941 gttcccgaga ccgccgtcga ttatacgctt tacggcgact ctgttttcct catcaccgag
     9001 aagaaagcgg atgacggcaa gaccagcctg accgccgttc gcaccttcgt gcggagcggc
     9061 aatcggatcg gcggacgcgc cgaaatcctc agcggcctga agcccggcga tcgcgtggtc
     9121 gccgtcggtc agctcaagct gcaatcggga gcggcagtgg ccatctcaac cgacgcgccg
     9181 ccgccgattc cggcgaatcc gccgcgttac taagagttaa agaatcggag cgtcgcgatg
     9241 gtctggaccg atattttcat caagcgaccg gtgttgtccg tggtcgtcag cctgctgatt
     9301 ctgctgatcg gctttcgggc cgcgaccacg ctgccgatcc ggcaatatcc gaaactgtcg
     9361 aatacggttg tcaatgtcac caccgtttat cccggcgcat cggccgatct gattggggga
     9421 ttcatcacga cgccgatcga gcaggccgtc gcgtccgccg agggcgtcga ctacatgacc
     9481 tcgtcttcgg tgcaaggcac cagcacgatc caggtctaca tcaagctgaa cttcgacccg
     9541 aaccaggcgc tgaccgaagt cctggccaag gtgaattcgg tgaaatatct cattccaaag
     9601 gaatcgaacg atccgatcgt gaccaagacc accggtcaga ccacggcagt gatgtatctc
     9661 ggcttctcct ctgaggtgct gtcgggatcg gcgatctcgg actatctgac gcgggtggtt
     9721 caaccggtgc tttcgaccgt cgatggcgtt gcttccgccg atattctggg cggccagact
     9781 ttcgcgatgc gggtgtggct cgatcccgtt cgcatggccg ggcgcaacgt ctcgccggcc
     9841 gatgtcaacg ccgcgatcgc cgccaacaat tttcaggccg ctgcgggcca ggccaagggc
     9901 tacttcatcg tctccaacgt gtcgaccaat acggatcttc ggaacctcga tcagttcaag
     9961 cggatgatca tcaaatccaa ggatggcggc ttcgtccgga tcgaggacat cgcaaccgtc
    10021 gaactcgcgg cgcaaagcac cgacgccagt gtcgccttca acggcgagca cgcgatcttc
    10081 atcggcgtgc aggcgacgcc gcagggcaac ccgctgacgt tggtcaaggg cgtccgcgcg
    10141 ctgtttccgg aactggaacg caatctgccg ccgtcgatga aaatgaaggt ggcctacgat
    10201 tcgaccaaat tcattcaatc gtcgatcgac gaggtcgaga aaaccctggg cgaagcggtc
    10261 atcatcgttg tcgtggtgat cttcatgttc ctggcatcgt tccgttcggt gatcatcccg
    10321 gtcgtgacca ttccgctgtc gctgatcggc gtctgcagcc tgatgttgat cgcgggattc
    10381 agtttcaacc tgctgacgct gctggcgatg gtgctggcga tcggtctcgt cgtcgacgac
    10441 gccatcgtgg tggtggaaaa catccatcgg catctggagg aaggcaaacc tccggtgcag
    10501 gcggccatgc agggcgcgcg cgagatcgtc ggccctgtcg tctccatgac catcacgctg
    10561 gccgcggtgt acgcgccgat cggctttctc ggcggcctca ccggttcgct gtttcgcgaa
    10621 ttcgcgttca ccctggccgg ctcggtcatc gtttcgggtg tgattgcgct gacgctgtcg
    10681 ccgatgatgt gctcgatctt cctcaagaat gccgaggaag gacgatttgc caagatcatc
    10741 aacaatgttt ttggatcggt gactcgctgg tatggccggc ggcttgatcg ctcgctcgac
    10801 tatcgtccga tcaccggggt gtttgctctg accatcctcg gcctggtcgg gttcctttat
    10861 ttgcacacgt cgaaagaact cgcacctgaa gaagatcagg gcatcgtgtt ctcggtgatc
    10921 aaggggccca aatacgccaa catcgactat ctcgatttct atggcgagaa actcgacaag
    10981 gcgttttcca gcttccccga aaccgatctg cgtttcgcac tcaatggcat caacggtccg
    11041 cagaacggga tcgccggcat gctgctcaag ccatgggacg aacgcgagcg ctcgtcgatc
    11101 aagctgaagc cgctggtgca ggcgcagctc agtaagatcg aaggcgtcaa cgcgttcgcg
    11161 ttcaacctgc cgccattgcc cggcggtccc ggcggattgc cggtgcagat ggtgatcaat
    11221 tcgaccaacg gatttcagtc ggtctacgag gaaatgctga agctgaagga cgccgcgcgc
    11281 aagagcgggc tgttcatcgt ctccgacagc gatctcgatt tcaaccagcc ggtggtgcgg
    11341 gtgaagatcg accgctcgaa ggcgagcgac ctcggcatca ccatgcagaa tgtcggggct
    11401 tcgctggcga cgctgctcgg cggaaactac gtcaaccgct tcaacctcga aggacgatcg
    11461 tatcaggtca ttccgcaggt gccgcgcgac tggcggctgt cgcccgaggc gctcggcgga
    11521 tattatgtgc cgacggcgac cggccagcag gtgccgctgt cgacactggt atcgatcgaa
    11581 accgcgaccg atcccaatgc cctgacgcat tataatcaat tgaactccgc aaccttccag
    11641 gccgtgccga tgcccggcgt cacggtcggg caggcggtcg acttcctcga gggcgcggcc
    11701 aagaacctcc cggccggctt cagccatgac tatctggctg actcccgcca gtacgtgcag
    11761 gaaggcaatc agctcgcggt taccttcggc ttcgcgctga tcatcatctt cctggtgctg
    11821 gcggcgcaat tcgagagcct gcgcgacccc ctggtgatca tgatcagcgt tcccatggcg
    11881 atcgtcggcg cgttgattcc gctgttcttc ggcatggcga cgatgaacat ctacacccag
    11941 gttggattgc tcactctggt cggtctcatc accaagcacg gcatcctgat ggtggagttc
    12001 gccaacgagc tccagctcaa tgaaggtctc gacaagcgtt cggcgattga aatggcagcc
    12061 cgcatccggc ttcgtccgat cctgatgacc acggcggcga tggtcaccgg cttgctgccg
    12121 ctgctgaccg ccaccggcgc aggcgcggcg agccggttct cgatcgggct tgtcgtcgtc
    12181 tcgggcatgt cgatcggaac gctgttcacg ctgttcgtgt tgcccgcggt ttatgtggcg
    12241 atcgccaccg atcacagggc ggcggccgaa tccgaacgca gcaaggagat cgcggacttc
    12301 gatctggggc cgaaggcgct caagccgacc tgagcaacac ggggcggtgc ggacgaaatg
    12361 atcgggtaat cttcccgcac tgcagcaaga gacagcggct cctgttcttg ctaaaacggg
    12421 ctggatgagc atttcccttc accccgacac gcccgagggg cgaacgctgg gcgggtcgcc
    12481 cgcggcgacc gtggcgccgg aggtggggcc atccggcaca aaaatccgca gccggctgtt
    12541 caccaaatat gtcgcgctgt tcgtcgcggt ggtcggcatc gcgctgctgt ccaatgggat
    12601 tttcgaggtt ttcttctatt atcgcgagca caaggcggcc ctgatccgga ttcagcgcga
    12661 gcaggccgag gcagcggcgg cgaagatcgg ccaattcgtc aaggaaatcg aaagccaact
    12721 cggctggacc acccagctgc cctggtctgc cgggtcactg gagaacaggc ggttcgacgc
    12781 cttgcggctg ttgaagcagg tgcccgccat caccgaactg gcgcagctcg attccaccgg
    12841 caaggaacgc atgcgggtat cgcgcctcgc catggatgtc gcggattccg gccttgacct
    12901 ctcaaaggac ccgaaattca ccgaggccgt cgcgcacaag gtctattatg ggccggtcta
    12961 tttccgccgg gagtccgagc cctacatgac gatcgcgctg gccggcaccc gcaaggatgc
    13021 cggcgtcagc attgccgagg ttaatctgaa gctgatctgg gacgtggtct cccagatcaa
    13081 ggtcggcgag cgcggtcacg cttatgttgt cggcgcggaa ggacgcctga tcgcgcatcc
    13141 cgatatcagc ctggtgctgc gcaacaccga tatgtccagg ctcgcgcagg tgcaggccgc
    13201 gctggcagcg ttagctgcgg tgctggccgc gcaggcagaa caggcagcgc tgaccgccca
    13261 ggcggcgcag actgggcagg cggcaccgcc acctcaggta ctgccggcaa cgccagcgtg
    13321 gacagcgaat tccggtcccg atgccttgca gggcgcgctg aatatccagg ggcaggaggt
    13381 gctgacggca tccgcgccga tcctgccgct gggctggatc atgttcgtcg agcttccggt
    13441 caaggaagcc tatgcttcgc tgtacgcggc gctgcagcgg ctggccacgg tgctggcggc
    13501 tgcgttgatc ttcgcggtgc tcgccgggat atttttggcc cggcgcatgg tcggcccgat
    13561 ccaggccttg cgcgccggcg ccgagcgtat cggcggcggc gatttcgccc agcgtatctc
    13621 gattcggacc ggcgacgagt tggaagggct cgccaaccag ttcaacgata tgggcacgcg
    13681 gctgcaggaa tcctatgcgg atttggagaa caaggttgag acgcgaacgg ctgaattgac
    13741 cgagtcactg gagcagcaga ccgcgacttc ggaggttctc gaggccatca gctcttcggc
    13801 tggcgaattg gatccggttt tccggaaaat gctcgaaaat gccacgcgca tctgcggagc
    13861 aaattttggc cagatgaatc tttacgagga ggggagctac cgtcccgttg cgcactacaa
    13921 tgtgccagcc gcctacgccg cctcgctggc gcacacgctc ttccaaccgc acccgcaaag
    13981 tggtctcggc accgttgccc gaacccacca agtggtccac atcgaagata ttcgagccct
    14041 gccgccgtat ctggagggca atccttccgt tgttgcacta gccgattttg ctggggcccg
    14101 gacatatttc gtcgtgccga tgctcaagga aaacgagttg atcggcgcta tcacgatttt
    14161 ccgccagcag gtcaagccgt ttactgaaaa gcagacggag cttgtcgcca acttcgccaa
    14221 gcaggccgtc atcgccatcg agaatacgcg cctgctcaag gagctgcgtc aacgcaccgg
    14281 tgatttgacc gagtcgctgg aacagcagac ggcgacatcg gaagtgttgc aggtcatcag
    14341 ttcatcgccg ggcgaattga agccggtgtt ccaaaagatg ctcgagaacg ccgtccgtgt
    14401 ctgcggcgcg aatttcggca cgatgaatct gtgggatggg gatagcttta atatcgttgc
    14461 agatcacaac gtcccacctg cgttcgcggc gttgcggcaa cgaatgccga tccgtccgca
    14521 tccggacacg cctatggccg ctgtcgtcag gacgcatcaa gtctttcatg ctcatgattt
    14581 gaaagacaat ccggcctacc tggccggcag gtccaacgtg gtcaacatgg tcgatatcgc
    14641 cggtgcccgc acgatcgtag tcgtgccaat gctgaaggaa gatgagctga tcggcgtgat
    14701 ttctattttc cgccaggaag tgcggccgtt caccgacaaa cagatcgcgc tggtcgaaaa
    14761 tttcaccaag caagccgtca tcgccatcga gaacacgcga ctgctcaggg aattgcgcga
    14821 acgcaccgac gatttgagcg aatcgctgca gcagcagacc gcgaccgccg atgtcctaaa
    14881 agtcatcagc cgctcggcat tcgatctgaa atccgtgctc acgacactgg cggagtcggc
    14941 gaagtcgctg tgcggcgcgg ctttcggcgt gatcttcctg cgcgacggcg aggtgctgcg
    15001 ggatcaggcg gaatcgggtt gtccgcccgc tctgatgaat tttctgcgcg accacccgat
    15061 ccgcaagagc cgtgcgacct ttaccggccg cgtcttcatg gatggcaagg ttgcgcacct
    15121 accggatgta ctgctggatc ccgagtacaa tttcggggcg gcgccggaac tcggcggcta
    15181 tcgcgccgcg ctcgccgtgc cgctgatgcg cgatggcggt gttgaaggcg tgctctcgct
    15241 ggcaaggccc gaccctggcc cgtttacatc gagacagatc gaactggtgc agaccttcgc
    15301 cgaccaggcc gtgatcgcca tcgagaacgc ccgattgttc gggcaggttc aggagcgaac
    15361 ccgtgaattg tcgctctcgc tcgacgagtt gcgcaccgcg caggatcgcc tggtgcagac
    15421 cgaaaagctc gcctcgctcg gccagctcac cgccggcatc gcccatgaga tcaagaatcc
    15481 gctcaacttc gtcaacaatt tttccgcact ctcggcggaa ctgattgacg aactgaacga
    15541 tgtgctcaag cccgccgcgc tcgacggcaa gatgcgggag gagatcgacg aactcaccca
    15601 tatgctgaag ggcaatctcg aaaaggtggt gcagcacggc aagcgcgccg actccatcgt
    15661 caagaacatg ctgctgcatt cccgcgaagg gtcgggcgaa caccggcc
//
LOCUS       DATNOI010000150         6180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2244476,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000150 DATNOI010000000
VERSION     DATNOI010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2244476"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VN968_05740"
     CDS             <1..678
                     /locus_tag="VN968_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HXL28775.1"
                     /translation="PATRARIGIVDRLFSRVGAADDLARGRSTFMVEMVETAVILNQA
                     SERSLVILDEIGRGTATFDGLSIAWAAIEHLHEANRCRALFATHYHELTALSAKLPRL
                     FNATVRVKEWQGDVVFLHEVLPGSADRSYGIQVAKLAGLPASVIARAKSVLAKLEAQD
                     RGQTARALADDLPLFAVPSRAAVEAPPPSEAEKLIEALNALHPDEMSPRDALEALYAL
                     KAKLPKQ"
     gene            complement(789..1583)
                     /locus_tag="VN968_05745"
     CDS             complement(789..1583)
                     /locus_tag="VN968_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28776.1"
                     /translation="MSPDLAFILTLALRMAVTAAFVVSASVITERSGPAIGALIATLP
                     ISAGPSYVFLALDHNAAFIAEGALASLPINAATIFLGLTYVVLAQRQSAIVSCLSAVA
                     VWIALASVIRTIHWSLAGGLIANAVAFAICVPLLRRFRNVKMPLITRRWFDIPLRASL
                     VATLVATVVTLSGWVGPTVSGIIALFPVVFTSMMLILHPRIGGPATAAVLANSGWGLM
                     GLGIAIAVLHVAALRFGSAVGLGLALMVCVGWNLGLWWNGRRKRVR"
     gene            complement(1744..2253)
                     /locus_tag="VN968_05750"
     CDS             complement(1744..2253)
                     /locus_tag="VN968_05750"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXL28777.1"
                     /translation="MDAAELRATQAPIKDRYKTDPKTAMITLKAKGSIENEGIACKVE
                     TGRALAVAGLHPATGGSGLELCSGDMLLEALVACAGVTLKSVATAIEVPLKTGLVTAE
                     GDLDFRGTLGVDKEAPVGFAEVRLRFDVDTSAPQDKLDLLLKLTERYCVVYQTIKNGP
                     KVSVTMHRV"
     gene            complement(2309..3094)
                     /locus_tag="VN968_05755"
     CDS             complement(2309..3094)
                     /locus_tag="VN968_05755"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28778.1"
                     /translation="MLVLDRLGKIYPNGVNALERFSAEIRLGEIVAIIGGSGCGKSTL
                     LRAIAGLDRATTGTVTLDETAITGPHAKIGIIFQEPRLLPWLSVADNIGFGLSEAPSH
                     VRREKVARALARVGLADKANAWPRELSGGQAQRVAIARALVPQPEVLLLDEPFSALDA
                     FTRRDLQDHLLDLWADTRPTLVLVTHDVDEAVVLADRVLVMRPRPGRLFDEIRINLAR
                     PRDRNSPLFDNFKRRVLTALDRSLDRSVPDTAAKSNAGEAMWW"
     gene            complement(3094..3939)
                     /locus_tag="VN968_05760"
     CDS             complement(3094..3939)
                     /locus_tag="VN968_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL28779.1"
                     /translation="MTVELPALEQADTMAPHAASGRLRRLARPALGLLLPVGLALAWE
                     IIVWLGLSSGRLVPPPTRLFATIVELARSGELARHIMATLTRVIAGFGLGVISGTVLG
                     AISGYWGLARRLLDPTVQALRAIPSLAWVPLFILWLGIFETSKIALIAVGVFFPVYLG
                     VMGAILSVDRKIVEVGRTFRLSGPAMIRRILLPAVLPAYVVALRVGLGLGWMFVVAAE
                     LMGASEGLGYLLLDGQQLGKPAQILAAIVIFAILGKTTDWLIELGAAPLLRWQDAFGR
                     QGGAA"
     gene            complement(4134..5108)
                     /locus_tag="VN968_05765"
     CDS             complement(4134..5108)
                     /locus_tag="VN968_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HXL28780.1"
                     /translation="MPKISRRALAAIIAVAALLPGGAYAAEPLKEIHIDWATYNPVSM
                     ILKQKGLLEKEFAKDGIGIVWVQSAGSNKALEFLNAGSIDFGSTAGSAALVARINGNP
                     IKSIYVYSRPEWTALVTAKDSKIAAVADLKGKRVAVTRGTDPHIFLVRALLDAGLSEK
                     DITPVLLQHADGKTALIRGDVDAWAGLDPMMAQSEVEEGARLFFRKPDANTWGILNVR
                     EQFLKDYPDVVRRVLATYEEARKYSVANYDDLKKTFMAITKLPDAVVDKQLKERTELT
                     HSRIGAPQRESMLAAGLALQQAGVIDAKVDVKATLDGLIDDQVPLPTN"
     gene            5384..>6180
                     /locus_tag="VN968_05770"
     CDS             5384..>6180
                     /locus_tag="VN968_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXL28781.1"
                     /translation="MDSVVTHPRPAVDERFDTARITAAIDALAATHAGREDVFRAAMA
                     QLLKAEMIQARAVAQAVLLQDRHGRRCAERLCFVQDEIIRILYAAATRHLYRSPIPSG
                     AERMAVVATGGYGRGLMAPESDIDLLFILPYKQTAWGEQVAEAILYCLWDMGLKVGHA
                     TRSVDESIRQARGDMTIRTAILETRFLTGDRPLYDELVERFDKEVVQGTAAEFVTAKL
                     AEREERHRRAGQSRYLVEPNVKDGKGGLRDLHTLFWIAKYVYRVRET"
BASE COUNT         1182 a         2019 c         1981 g          998 t
ORIGIN      
        1 ccggcgacgc gggcgcggat cggcattgtc gaccggctgt tctcacgcgt cggcgccgcc
       61 gacgacctgg cacgcggccg ctccaccttc atggtggaga tggtggagac cgcggtcatt
      121 ctcaatcagg ccagcgagcg ctcgctggtc attctcgacg agatcggccg tggcaccgcg
      181 accttcgacg gcctgtcgat cgcatgggcc gcgatcgagc atctgcatga agccaatcgc
      241 tgccgcgcgc tgttcgccac gcactatcac gaactcaccg cgctgtcggc aaaactgccg
      301 cggctgttca atgccacggt gcgggtcaag gaatggcagg gcgacgtcgt gttcctgcat
      361 gaagtgctgc cgggctcggc cgaccgctcc tacggcatcc aggtggcaaa gctcgcagga
      421 ctgcccgcat cggtgattgc ccgcgccaaa tcggtgctcg caaagctcga ggcgcaggat
      481 cgcggccaga ccgcacgcgc attggccgac gacctgccgc tgttcgcggt gccgtcacgc
      541 gcagcagtcg aggcgccgcc gccgagcgag gcggaaaagc tgatcgaagc gctgaacgcc
      601 ctgcaccccg acgaaatgtc gccgcgcgac gcgctggaag cgctgtatgc attgaaggcg
      661 aaattgccga aacagtaact gctcctcatc ctgaggagcc gcgaagcggc gtctcgaagg
      721 atgcagcaca acgggccgca tggttcgaga cgcgcgaaga cgcgctcctc accatgagga
      781 cgaccttctc accgcacgcg cttcctccgc ccgttccacc acaggccaag attccagccg
      841 acgcagacca tcagcgccag accgaggcca accgccgagc cgaaccgaag tgcggcgaca
      901 tgcagcacgg caatcgcgat gcccagtccc atcaaccccc agccgctgtt cgcgagcacc
      961 gcggcggtcg ccggcccgcc gatgcgcgga tgcaaaatca gcatcatgct ggtgaacacc
     1021 accgggaaca gcgcaatgat gccgctcacg gtcgggccaa cccagcccga cagcgtgacc
     1081 accgtcgcca ccaacgtcgc aaccagcgac gcgcgcagcg ggatatcgaa ccagcggcgc
     1141 gtgatcagcg gcattttgac gttgcggaag cgccgcagca gtggcacgca gatcgcaaaa
     1201 gcaacggcat tcgcgatcag gccgccggcg agcgaccagt ggatcgttcg tatcaccgaa
     1261 gccagcgcga tccacacggc gaccgcgctc agacaactga caatggcgct ctggcgctgc
     1321 gccagcacga cgtaggtcag accgaggaag atcgtcgcgg cgttgatcgg caggctcgcc
     1381 agtgcgcctt cggcgatgaa cgccgcattg tgatcgagcg cgagaaaaac gtaagagggg
     1441 cctgccgaaa tcggcaatgt cgcgatcagc gcgccgatcg ccggccccga gcgctcggtg
     1501 atcacagaag ccgaaaccac gaacgccgcc gtcaccgcca tgcgcagtgc gagcgtcagg
     1561 atgaaagcga ggtcggggga catgggccta gccgtcgtcc cggcgaacgc cgggacccat
     1621 agccaccgta ttcacgattt aggcggcggc gtcagaaaag catcggttga ggcaccgagg
     1681 cgcaaacgag aggacaggga gtatgggtcc cggcgttcgc cgggacgacg acggattgtg
     1741 cgatcacacc cgatgcatcg tcaccgacac cttggggccg ttcttgatgg tctggtagac
     1801 cacgcaataa cgttcggtaa gtttcagcag cagatcgagc ttgtcctgcg gcgctgacgt
     1861 atcgacgtcg aaacggagac ggacttcggc gaagccgacc ggggcctcct tgtcgacgcc
     1921 gagcgtgccg cggaaatcga gatcgccctc ggccgtgacg aggccggtct tcagtggcac
     1981 ctcgatcgcg gtcgccaccg acttcaacgt cacgccggcg caggcgacca gcgcttccag
     2041 cagcatgtcg ccggagcata attcaagccc agaacctccg gtggccggat gcaggccggc
     2101 gaccgccagc gcgcggccgg tctcgacctt gcaagcgatg ccctcgttct cgatcgagcc
     2161 tttggctttc agggtgatca tggcggtttt tggatcggtt ttgtagcgat ccttgatcgg
     2221 agcctgcgtg gcgcggagtt cagcagcgtc cattttttgt tctccagaat aaattgcgcg
     2281 tcggatgtaa cgggtcagag ggcagatgtc accaccacat cgcctcgccg gcgttggatt
     2341 tcgccgcggt atcgggcacg ctgcggtcga gcgagcgatc cagcgcggtc agcacgcggc
     2401 gcttgaaatt gtcaaacaaa ggcgaattgc ggtcgcgcgg acgcgccaga ttgatcctga
     2461 tctcgtcgaa caggcggccc ggccgcggcc gcatcaccag gacgcggtcg gccagcacca
     2521 cggcttcgtc gacatcgtgc gtcaccagca ccagggtcgg ccgcgtatcg gcccagaggt
     2581 cgagcagatg atcctgcaaa tcccgccgtg tgaacgcatc gagcgcggag aacggctcgt
     2641 ccagcagcag cacttccggc tgcggcacca gcgcgcgggc gatcgccacc cgctgcgcct
     2701 gcccgccgga tagttcgcgc ggccaggcat tggccttgtc ggcaagaccg acccgcgcca
     2761 atgcgcgtgc gaccttctcg cggcgaacat ggcttggggc ttccgacagg ccgaaaccga
     2821 tattgtcggc aacactgagc cagggcaaca gccgcggctc ctgaaagatg atgccgattt
     2881 tcgcatgggg gccggttatc gccgtctcgt cgagtgtcac ggtgcccgtt gtggcgcggt
     2941 cgaggccggc gatggcgcgc agcagcgtcg actttccgca accggagccg ccgatgatgg
     3001 cgacgatctc ccccaacctg atctcggcgg aaaaacgctc cagcgcattg acgccgttgg
     3061 gatagatctt gccaagccga tcgagcacca gcatcaggcc gctccgccct ggcggccgaa
     3121 ggcgtcctgc cagcgcaaca gcggtgctgc gccgagctcg atcagccaat cggtggtctt
     3181 gccgagaatg gcgaagatca cgatcgccgc cagaatctgc gcgggcttgc cgagctgctg
     3241 gccgtcgagc agcagatatc ctagtccctc ggatgcgccc atgagctctg cggcgaccac
     3301 gaacatccag cccaaaccga ggcccacccg cagggcgacg acataggccg gcaacaccgc
     3361 gggcagcagg atgcgccgga tcatcgccgg tccggagagg cggaaggtgc ggccgacttc
     3421 gacgatcttg cgatcgaccg agaggatcgc gcccatgacg ccgagataga ccgggaaaaa
     3481 taccccgacc gcgatcagcg cgattttcga ggtctcgaaa atgccgagcc acaggatgaa
     3541 cagcggcacc caggcgagcg acggtatcgc gcgcagcgcc tgcacggtgg gatcgagcag
     3601 ccggcgcgcc aaaccccaat agcccgagat ggcgccgagc acggtgcccg atatcacgcc
     3661 gaggccgaag ccggcgatga cgcgcgtcag tgtcgccatg atatggcgcg cgagctcacc
     3721 gctgcgcgcc agttcgacaa tggtggcgaa cagtctggtc ggtggcggca ccaaccgtcc
     3781 gctggacagg ccgagccaga cgatgatctc ccaggccagc gcgaggccaa ccggcagcag
     3841 cagtcccagc gccggccgcg ccagccgtcg cagacgaccg gacgccgcat gcggcgccat
     3901 cgtatcggct tgttcaagcg cgggcagttc aacggtcatg gccatagcaa gacgcttctc
     3961 gagcatgaat gcaagtcggc gaatgggagg acgcgcactc gctgctggtc gtccccgcga
     4021 acgcggggac ccataaccac cggccgtcat ggtgaacaaa ggcgtcgacc atcgagtaaa
     4081 acagcaaggc cgcggcgtat gggtccccgc gttcgcgggg acgaccacac ccctcaattc
     4141 gtcggcagcg ggacctggtc gtcgatcagg ccgtcgagcg ttgccttgac gtcgaccttg
     4201 gcgtcgatca cgccggcctg ctgcagcgcc agtcctgccg ccagcatgga ctcgcgctgc
     4261 ggcgcgccga tccggctatg ggtcagttcg gtgcgctcct tgagctgctt gtcgaccacc
     4321 gcgtccggca atttggtgat ggccatgaag gtcttcttca ggtcgtcgta attcgccacc
     4381 gaatatttgc gcgcctcctc gtaagtggcg agcacgcggc gaaccacgtc cggataatcc
     4441 ttcaggaact gctcgcgcac attgaggatg ccccaggtat tggcgtcggg cttgcggaag
     4501 aacagcctgg cgccctcctc cacttccgac tgcgccatca tcggatcgag acccgcccag
     4561 gcatcgacgt cgccgcggat cagcgcggtc ttgccgtcgg catgctgcag cagcaccggc
     4621 gtgatgtctt tttcgctgag gcccgcgtcg agcagcgcgc gcaccaggaa gatgtgcgga
     4681 tcggtgccgc gggtcaccgc gacgcgcttg cccttgaggt cggcgacggc tgcgattttc
     4741 gaatccttgg ccgtcaccag agccgtccat tccgggcgcg aatagacata gatcgacttg
     4801 atcgggttgc cattgatcct ggcgacaagg gccgcggagc ccgcggtcga gccgaaatcg
     4861 atcgatccgg cgttgaggaa ctcgagcgcc ttgttggagc cggccgattg cacccagacg
     4921 atgccgatac cgtccttggc gaattccttt tcgagcagcc ccttttgctt caggatcatc
     4981 gacaccggat tgtaggtcgc ccaatcgatg tggatttcct tgagcggctc ggccgcgtag
     5041 gcgccaccag gcagcagcgc cgcgacggca atgatcgcag ccagggcacg ccgtgaaatc
     5101 ttgggcatcg ttgttatctc catgaaagtg gaatgatcaa agcggatcgc aaggaagcat
     5161 cggcgcggca ggattctctt gccggcgcag caccctgctc gctttaaaaa cgctctttta
     5221 tttcaatgag ttaaatgaac gcgtcaacga gaaaatacgt acgtccaacg cttcccgctg
     5281 agaatagctt tgctcaaagc cgagggaacg cagcatggac cttctgtcat cttcgggaat
     5341 gttcccgtga cagggtcggc ggcgtccgat atcgggatag gccatggaca gcgtcgtgac
     5401 acacccccgg cccgcagttg acgaacgttt cgataccgcg aggatcaccg ccgcgatcga
     5461 cgcgctcgct gcgacccacg ccggacgcga ggatgttttc cgcgccgcca tggcgcagct
     5521 gctgaaggcc gagatgatcc aggcccgcgc ggtggcgcag gcggtgctgc tgcaagatcg
     5581 ccacggacgg cgctgcgccg aacggttgtg tttcgtccag gacgaaatca tccgcattct
     5641 ctacgccgcc gccacgcgtc atctgtatcg ctcgccgatc ccctcgggcg ccgagcgcat
     5701 ggccgtcgtc gccaccggcg gctatggccg cggactgatg gcgccggagt ccgatatcga
     5761 tctgttgttc atcctgccct acaagcaaac cgcctggggc gagcaggtcg ccgaggccat
     5821 tctgtattgc ctgtgggaca tgggcctgaa ggtcggccac gcgacgcgct cggtcgatga
     5881 atcgatccgg caggcgcgcg gcgacatgac catccgcacc gcgatcctcg agacgcggtt
     5941 cctgaccggg gatcggccgc tctatgatga actggtggag cggttcgaca aggaggtggt
     6001 gcagggcacc gcagccgaat tcgtcaccgc caagctcgcc gagcgcgagg agcgacaccg
     6061 ccgcgccggg caatcgcgct atctggtcga gcccaacgtc aaggacggca agggcggctt
     6121 gcgcgatttg catacgctgt tctggatcgc gaaatatgtt taccgcgtgc gcgagaccga
//
LOCUS       DATNOI010000151         2325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1875139,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000151 DATNOI010000000
VERSION     DATNOI010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875139"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            344..1213
                     /locus_tag="VN968_05775"
     CDS             344..1213
                     /locus_tag="VN968_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL28782.1"
                     /translation="MTTLFTILFDGVAYGMLLFVLACGLAVTLGLMNFVNLAHGAFAM
                     AGGYVCAVLVNRSGWPFFASLPLAFVASAVIGAVLERALYRHLYNRSHLDQVLFSVGL
                     VFMSVAAVDYIMGSSRIFIKLPAALEGQFDLFGVGIGRYRLMIIVICGLLTAGLQLVL
                     ARTRFGSRLRAAVDDPRAAIGLGINVPQVFAFTFAFGCGLAGLGGALSAEILGLDPYF
                     PLKFMIYFLIVVTVGGSSSITGPFLASLLLGIGDVAGKYYVPKMGPFVIYTIMIVILI
                     WRPNGLFGRTAAR"
     gene            1222..2253
                     /locus_tag="VN968_05780"
     CDS             1222..2253
                     /locus_tag="VN968_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL28783.1"
                     /translation="MTAQADVGHQARQHARWHWSEIAFWILALSCAFLFPSRYLIMTD
                     IVRLALFALSLDLILGYAGIVSLGHAAFFGVGAYSAGLLALHGIVTEPVIALVVAGLV
                     AMAVGFLTSFLVIRGADLTRLMVTLGIALLLEALAERFSNITGGTDGLQGIEMRPILG
                     LFAFDMFGKVGFFYCLAVLFLLFLLARRIVHSPFGLSLRAIRNNPLRAAAIGIPVNRR
                     LIAIYTLAAFYAGIAGALFTQTTALASLDVFSFERSADLMLVLVIGGTGYLYGGLIGA
                     VVFRMLQEVFSTITPQYWQFWIGLVLVVIVLVGRARMHRWALWAPNLIIRQLGGRKAV
                     VAVPESDAP"
BASE COUNT          332 a          723 c          738 g          532 t
ORIGIN      
        1 gaacatctac atccgcaagg tcgagaaggt cgatggcgaa ctctacaacg tcgaattcgc
       61 gaccttcgag gccgtcaagg acgccggcaa gacgaagaag tgacgtccgt gcttgcggct
      121 tgcccctcac cccaaccctc tccccgcaag agcggggcga gggagcaggg cggtattcgc
      181 ggcataaact tcgcttcggc agagggtcgg caatgaactg cgacttgctc ggtgctaacc
      241 tcgccccgct cttgcgggga gaggtcggat cgcgcagcga tccgggtgag gggctcttga
      301 acgctcggac gattttgctc gatctcagat cagcgaaccc aacatgacca cgctgttcac
      361 catcctgttc gacggcgtcg cctatggcat gctgctgttc gtgctggcct gcggcctggc
      421 cgtcacgctc ggactgatga attttgtcaa tctggcacac ggggcgttcg ccatggcggg
      481 cggctatgtc tgcgcggtgc tggtcaatcg atccggctgg ccgtttttcg caagcctgcc
      541 gctggcgttt gtcgcaagtg ctgtgatcgg cgcggtgctg gagcgcgcgc tctatcgtca
      601 cctctataac cgcagccatc tcgatcaggt gctgttcagc gtcggcctcg ttttcatgtc
      661 ggtggcggcg gtcgattaca tcatgggatc gtcgcggatc tttataaaac ttccggccgc
      721 gctcgaaggt caattcgacc tcttcggggt cggtatcggc cgctaccgcc tgatgatcat
      781 cgtgatctgt ggtctgctga ctgcaggcct gcagctggtg ctggcgcgca ccagattcgg
      841 cagccggctg cgcgcggcgg tggacgatcc gcgcgcggca attgggctcg gcatcaacgt
      901 gccgcaggtg tttgcattta ccttcgcctt cggctgcggc cttgctggtt tgggcggcgc
      961 cctgagcgcg gagattttgg ggctcgatcc gtattttccg ctgaagttca tgatctactt
     1021 cctgatcgtg gtgaccgtcg gcggctcctc cagcatcacc gggccgttcc tggcgtcgct
     1081 gctgctcggc atcggcgacg tcgccggcaa atattacgtg ccgaaaatgg ggccgttcgt
     1141 catctacacc atcatgatcg tgatcctgat ctggcgcccg aacggcctgt tcggccgcac
     1201 cgccgcccgt tgagcctgcc catgaccgcg caagccgacg tcggacacca ggccaggcag
     1261 cacgcgcgct ggcactggag cgagatcgcg ttctggattc tcgcgctttc ttgcgcattc
     1321 ctgtttcctt cccgctatct gatcatgacg gatatcgtgc ggctggcgtt gttcgcgctg
     1381 tcgcttgatc tgatcctcgg ctacgccggt atcgtttcgc tcggccacgc cgcattcttc
     1441 ggtgtcggag cctattccgc cgggctgctt gccttgcacg gcatcgtcac cgagccggtg
     1501 attgcgctgg tcgtggccgg cctcgtcgcc atggcggtcg gctttctcac cagtttcctg
     1561 gtgatcaggg gcgccgacct gacccgcctg atggtgacgc tcgggattgc gttgctgctg
     1621 gaggcgctgg cggagcgttt ttccaacatc accggcggca ccgacgggct gcagggcatc
     1681 gagatgcggc cgatcctggg cttgttcgcg ttcgacatgt tcggcaaggt cggcttcttc
     1741 tactgcctcg ccgtgctgtt cttgttgttc ctgctggcgc ggcggatcgt gcactcgccg
     1801 ttcggcctgt cgctgcgtgc tatcagaaac aatccgctgc gcgccgctgc gatcggcatc
     1861 cccgtcaacc ggcgcctgat cgcgatttat acgcttgccg cgttctatgc cggcatcgcc
     1921 ggcgcgctgt tcacccagac cacggcgctg gcctcgctcg acgtgttctc gttcgagcgg
     1981 tctgccgacc tgatgctggt gctggtgatc ggcggcaccg gctatctcta tggcggattg
     2041 atcggcgccg tggtgttcag aatgctgcag gaagtgtttt cgaccatcac gccgcaatac
     2101 tggcagttct ggatcggcct tgtgctggtc gtgatcgtgc tggtcggccg ggcgcgcatg
     2161 catcgctggg cgctgtgggc gccgaacctg atcatccggc agctcggcgg gcgaaaagct
     2221 gtcgttgccg tccctgagag cgacgcgcca tgagtatcgc gctggaaacc aaagggctgc
     2281 acaagcagtt cggcgggctc atagtcacgc gcgatctttc gctta
//
LOCUS       DATNOI010000152         9970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1155132,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000152 DATNOI010000000
VERSION     DATNOI010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9970
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155132"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VN968_05785"
     CDS             complement(<1..942)
                     /locus_tag="VN968_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HXL28784.1"
                     /translation="MKTAKDVLKSIKDNDVKYVDLRFTDPRGKWQHVTFDIGMIEEDT
                     FAEGLMFDGSSIAGWKAINESDMCLMPDPVTATIDPFFAETTMVITCDVLEPTTGEPY
                     NRDPRGMAKKAEAMVKSMGVGDTVFFGPEAEFFVFDDVRFQTTPYNTGFKLDSSELPI
                     NSDTEYEGGNLGHRIRTKAGYFPVPPQDSVQDMRSEMLGAMAKMGVKVEKHHHEVASA
                     QHELGMKFDTLTLMADQMQIYKYCIHQVAHIYGKTATFMPKPVYGDNGSGMHCHQSIW
                     KEGKPVFAGNKYADLSETCLSYIAGVIKHAKAINAFTN"
     gene            complement(1076..1414)
                     /locus_tag="VN968_05790"
     CDS             complement(1076..1414)
                     /locus_tag="VN968_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXL28785.1"
                     /translation="MKKIEAIIKPFKLDEVKEALQEVGLQGITVTEAKGFGRQKGHAE
                     LYRGAEYIVDFLPKVKIEIVIGDDLVERAIDAIRRAAQTGRIGDGKIFVSNIEEAIRI
                     RTGESGLDAI"
     gene            1806..3305
                     /locus_tag="VN968_05795"
     CDS             1806..3305
                     /locus_tag="VN968_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HXL28786.1"
                     /translation="MEVLDTAEMERADRLTIAAGTPGFALMLSAGQAVAEAAIDLVAE
                     GPMLVVAGGGNNGGDGFVAAAELAARGREVSVILLCERDSLRGDAASAARGWKYPVLP
                     CNPQAIGKPALIIDALFGAGLNRPVKGDSYDMIEAINANCAPVLSVDLPSGINGSTGA
                     MMGVAVRATETVTFFRRKPAHLLLPGRIHCGRVRVADIGIDASVLEEIRPKTFENTPQ
                     FWRKSFPVPQIDGHKYARGHAVIVSGDITATGAARLSARGALRAGAGLVTLASPRDAL
                     AVNAAALTAVMVRAIDTVVEFGELLTDKRLKTCVIGPGAGVGERTRDLVLTALSAKRH
                     LVLDADALTSFADAPDRLFEAIRASQDQQVVLTPHEGEFPRLFSDISNKHPYRSKLER
                     VRAAAERCGAIVLLKGPDTVVASPDGRATISANAPPWLATAGAGDVLAGMIAGLLAQG
                     VAGFEAASTGAWMHGEAAREAGPGLIAEDLPEVLPGVFRRLYDEFGIDY"
     gene            complement(3449..4792)
                     /locus_tag="VN968_05800"
     CDS             complement(3449..4792)
                     /locus_tag="VN968_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HXL28787.1"
                     /translation="MVRIDLMADSIFPLDRRDVIAGLGAVVLSPAMPSIATAQRQSPS
                     LTLRAGADVIALRPARPDTPIWSLGGPAPDRDIGLKRGDALEITLLNDLPVPTVLNWH
                     GIEGVAAAEPLAARPPLAPGAKETLVIPLRHAGTLMIDLRLLGDGQARPSSARALIVG
                     ESEPVSVDRDQVFLIEDWRLRPDGTAIAPGVDPKDTVPIYTINGLSTLDIPTRTNERL
                     RLRFINGCQRSVIAIKIEGHEVRVMAIDGQPAEPFPARNGAVVLAPGTRVDAFIDATA
                     PPGSTSLILLHDGKEPRPIGRLIASGEPQIRAAPLPLAPPLPSNGQPTQLDLKNALRF
                     DMALGGPQTDWATPATFAVTAPPSFRAKVGRTVVLALTNRAEIATVFHLHGHPFRLLD
                     RLDDGWKPFWLDTLAVEPGQTQRIAFAAEHAGRWLIESTKTDWAAPRLVRWCSVE"
     gene            4929..5013
                     /locus_tag="VN968_05805"
     tRNA            4929..5013
                     /locus_tag="VN968_05805"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Leu,seq:tag)
     gene            5188..6549
                     /gene="tig"
                     /locus_tag="VN968_05810"
     CDS             5188..6549
                     /gene="tig"
                     /locus_tag="VN968_05810"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXL28788.1"
                     /translation="MQVTETLAEGLKHEFQISVPASDLDAKADVRFVELKDKVRLNGF
                     RPGKVPVSHLKKVYGRSVMAETIDQTIRDTNSQIFTERGFRLATEPKVTMPTEQKAVE
                     DILTGKSDLTYTVSIEVVPTIALADFKSFAVEKPVVEVTGADVDEAIKRIADQNRSFA
                     AKGDGAKAENGDRVTISFKGTIDGAPFDGGTGENIQVTIGSNTFIPGFEEQLLGIGAG
                     ETRVLKVSFPKNYASEKLAGQPAEFETTATLIEAPQETAINDEFAKTLGLESLDKLKE
                     AARERLVAEFAGATRQRVKRMLLDRLDESHRFEAPPSLVEEEFNLMWNSIKAEMESGG
                     KTFADEDTTEEAAREEYHKIADRRVRLGLVLSEIGEKNKITVTDDEVSRAVIERARQM
                     PGHEKEVWDYYRNNNNALAQLRAPIYEDKVVDFILELANVTEKKVTREDLYKDDEAEK
                     SAA"
     gene            6843..7475
                     /locus_tag="VN968_05815"
     CDS             6843..7475
                     /locus_tag="VN968_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HXL28789.1"
                     /translation="MRDPVETYMQLVPMVVEQTNRGERAYDIFSRLLKERIIFVTGPV
                     EDGMSTLVVAQLLFLEADNPKKEISMYINSPGGVVTSGLAIYDTMQFIRPAVSTLCTG
                     QAASMGSLLLAAGQKDMRFSLPNSRIMVHQPSGGFQGQATDIMLHAQEILNLKKRLNE
                     IYVKHTGQTFKAIEDALERDKFLTAEMARDFGIVDKVIDKRSEDPMAKSP"
     gene            7886..9160
                     /gene="clpX"
                     /locus_tag="VN968_05820"
     CDS             7886..9160
                     /gene="clpX"
                     /locus_tag="VN968_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HXL28790.1"
                     /translation="MSKVGTSDSKNTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     MDIIREENKSSLVKSRDGIPTPKEICKVLDDYVIGQSHAKKVLSVAVHNHYKRLNHQT
                     KHNDVELAKSNILLIGPTGSGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVENI
                     ILKLLQSADYNVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIMEGTV
                     ASVPPQGGRKHPQQEFLQVDTTNILFICGGAFSGLEKIISARGRSTSIGFAAQVLAPE
                     DRRTGEIFRHVEPEDLLKYGLIPEFVGRLPVVATLEDLDEASLKKILTDPKNALVKQY
                     QRLFEMENIELTFADEALGAVARKAIERKTGARGLRSILESILLETMFDLPGLEGVEE
                     VVISREVVEGTARPLYIYADRSDRAVETSASA"
     gene            9471..>9970
                     /locus_tag="VN968_05825"
     CDS             9471..>9970
                     /locus_tag="VN968_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXL28791.1"
                     /translation="MTTPKPRPSIVHGESHSYPVLPLRDIVVFPHMIVPLFVGREKSI
                     RALEEVMKNDALIMLATQKNASDDDPAPDSIYETGTLASVLQLLKLPDGTVKVLVEGL
                     ERARVQKYSDRAEYYEAAAIALADTDATSVEAEALARSVVSDFESYVKLNKKISAEVV
                     GVVQAIT"
BASE COUNT         1931 a         2916 c         3173 g         1950 t
ORIGIN      
        1 gttggtgaag gcgttgatcg ccttggcgtg cttgatgacg ccggcgatgt aggacaggca
       61 ggtttccgag aggtcggcgt atttgttgcc ggcgaatacc ggcttgcctt ccttccagat
      121 cgactggtgg cagtgcatgc ccgagccgtt gtcgccatag accggcttcg gcatgaacgt
      181 ggcggtcttg ccgtagatgt gtgcgacctg atggatacaa tatttgtaga tctgcatctg
      241 gtcggccatc agcgtcagcg tgtcgaactt catgccgagt tcgtgctggg cggaggcgac
      301 ttcgtggtga tgcttctcga ccttgacgcc catcttggcc atcgcgccga gcatttccga
      361 gcgcatgtcc tgcaccgaat cctgcggggg aaccggaaag tagccggctt tggtccggat
      421 gcggtggccg aggttgccgc cctcatattc ggtgtccgag ttgatcggca gttccgacga
      481 atccagcttg aagccggtgt tgtagggtgt ggtctggaag cggacgtcgt cgaacacgaa
      541 gaattccgcc tcaggtccga agaacaccgt atcgccaacg cccatcgact tgaccatcgc
      601 ctcggccttc ttggccatgc cgcgcgggtc gcggttgtag ggctcgccgg tggtcggctc
      661 cagcacgtcg caggtgatca ccatggtggt ttctgcgaag aacggatcga tcgtcgcggt
      721 aaccggatcg ggcatcaggc acatgtcgga ttcattgatc gccttccagc ccgcgatcga
      781 cgagccgtcg aacatcaagc cttcggcgaa agtgtcctcc tcgatcatgc cgatatcgaa
      841 ggtcacatgc tgccacttgc cgcgcggatc ggtgaaacgc aggtcgacgt atttgacgtc
      901 gttgtccttg attgatttca ggacgtcttt ggcggtcttc atgaataccc ctttttggct
      961 tacaggtcgt cgtgatccgg ccgccggcag cgaccttggg tcatgctcat tacaattggg
     1021 gcaagttcaa tacaatatcc tcaagacaat atcggggggt gtcggcgggt tcgattcaga
     1081 tggcatccag cccggattcc cctgttctga tccgaatggc ttcctcgatg ttggaaacga
     1141 agatcttgcc gtcgccgata cgcccggtct gggccgcacg cctgatcgcg tcgatcgccc
     1201 gctcgaccag gtcatccccg atcacgatct cgattttgac cttgggcaga aagtccacga
     1261 tgtattccgc gccgcgatag agttcggcgt ggcctttctg ccgcccgaaa cccttggctt
     1321 cggtgacggt gatgccctgc agtccgacct cctgaagtgc ctctttcacc tcgtcgagct
     1381 tgaatggctt gatgatggct tcgatttttt tcactgaacg cctcccaggc acttccatat
     1441 ttcagattgc aggtttgttt ccacgtggcg cgtggagcgc gggttgttcg accgttgtcc
     1501 ttgcgaattg tccttaagaa ttgtcctggc gggcagccgg ggacacgtca ataaattgcc
     1561 cttgaggatg atcgtccaat gccggcttcc tcaaaagcag ggtctatgcc aagttgccaa
     1621 aacggctgat ttcgaaatgt tatcagactt ttaacaggta ttcacgtggg ggtgggctag
     1681 gcgattcacc atagttgagc ctatgaatta ggcaaaaaga tcaatcggcg tgcaatccgc
     1741 acggatttga tacagcacgg gatggaaaat gcctgaggat aaggcgcttg gatcggaaat
     1801 ttggcatgga agttcttgat acggctgaga tggagcgcgc cgatcggctg accatagccg
     1861 cgggtacgcc tggctttgcg ctgatgctga gcgcgggtca ggccgttgcg gaggccgcga
     1921 tcgacctggt ggcggagggg ccgatgctgg tggtcgccgg cggcggcaat aatggcgggg
     1981 acggtttcgt ggctgcggct gaactcgcgg cgcgcggccg cgaggtctcg gtcatcctgc
     2041 tgtgtgagcg ggacagcttg cgaggcgacg cagcctcggc ggcgcgcgga tggaaatatc
     2101 cggtgctgcc gtgcaatccc caggcgattg gcaagcccgc gctgatcatc gacgccttgt
     2161 tcggcgccgg tctgaaccgg ccggtgaagg gcgattcata cgacatgatc gaggcgatca
     2221 atgccaactg cgcgccggtt ctcagtgtgg acctgccgag cggcatcaac ggcagcaccg
     2281 gcgccatgat gggcgtcgcg gtccgcgcca ctgagacggt cacattcttc cgccgcaagc
     2341 cggcgcattt gctgttgccg ggccgaattc attgcggtcg cgtgcgcgtc gccgatatcg
     2401 ggatcgatgc gagcgtgctt gaggaaattc gtccgaagac cttcgaaaat acccctcagt
     2461 tttggcgcaa atcctttccg gtgccgcaga tcgacggcca caaatacgcc cgtggccatg
     2521 cggtcatcgt atccggcgat atcaccgcca ccggtgcggc gcggctatcg gcgcgcgggg
     2581 cgctgcgcgc cggcgccggg ctggtcacgc tggcctcgcc ccgggatgcg ctcgccgtca
     2641 atgccgctgc gctgaccgcc gtgatggttc gcgcgatcga cacggtggtc gagtttggcg
     2701 aactgttgac cgacaagcgc ctcaaaacct gcgtgatcgg gccgggtgcc ggcgtcggcg
     2761 agcggacccg cgatcttgtc ctgacggcgc tgtcggcaaa gcggcacctc gtgctggatg
     2821 cggatgcgct gacaagtttt gccgatgctc ccgatcgtct gttcgaagcg atcagggcat
     2881 cgcaggatca gcaggtggtt ctcactccgc atgaaggaga atttccgcgg ctgttcagcg
     2941 atatcagcaa caagcatcca tatcgctcga aactcgagcg cgtcagggca gcagccgagc
     3001 gctgcggcgc catcgttctg ctcaagggac cggacaccgt ggtggcatca ccggacgggc
     3061 gcgcgaccat ctccgccaac gcgccgccct ggctcgccac cgccggcgcc ggcgacgtgc
     3121 tggcaggaat gatagcgggc ctgctggccc aaggcgtggc cgggttcgag gccgccagta
     3181 ccggagcctg gatgcatggc gaggcggcgc gcgaggcagg ccccggtttg atcgcggaag
     3241 acctgccgga ggtgttgccc ggggttttcc ggcgcctcta cgatgagttc ggtatcgact
     3301 attgagacgg aaaatgccgc gtcacgtccg cgaaatgctt ggcccggccg aatcactgac
     3361 gggtgtgttc ggggacatgg ctgacacatc atcccgggtt ggattggtcc atttggggac
     3421 ccttcgaggc ccagatggtg ctcgcccctc actcgacact gcaccaccgc acgaggcgcg
     3481 gtgccgccca gtcggtcttc gtcgactcga tcagccagcg gccggcatgc tcggcagcga
     3541 atgcgatgcg ctgggtctgc ccgggctcga ccgccagcgt gtccagccag aacggcttcc
     3601 agccgtcatc gagccgatcc agcagcctga agggatggcc atggagatgg aacacggtcg
     3661 caatctcggc gcggttggtc agcgccagca ccacggtgcg gcccaccttg gcgcggaacg
     3721 agggcggggc ggtcacagcg aaggtcgccg gcgtcgccca gtcggtctgc ggcccgccta
     3781 gcgccatatc gaagcgcaag gcgttcttga ggtcgagctg ggttggctgg ccgttggatg
     3841 gcagcggggg tgccagcggc agcggcgcag cgcggatctg cggctcgccg gaggcgatca
     3901 gccggccgat ggggcgaggc tccttgccgt catgcagcag gatcaaagag gtcgagccgg
     3961 gcggagcggt cgcgtcgatg aaggcgtcga cccgggttcc cggtgccagc accacggcgc
     4021 cgttgcgggc cgggaacggt tcggcgggct ggccgtcgat agccatgaca cggacctcgt
     4081 gcccctctat tttgatggcg ataaccgacc gttggcagcc gttaataaag cgaagtctca
     4141 ggcgttcatt ggtgcgtgtt gggatatcca gcgtgctgag cccgttgatg gtgtagatgg
     4201 gcacggtgtc ctttggatcg acgccgggcg cgatcgcggt tccgtccggg cgcagccgcc
     4261 agtcttcgat cagaaacacc tgatcgcgat cgacgctgac gggctcgctc tccccaacga
     4321 tcagtgcccg ggccgacgac ggcctcgcct ggccatcgcc gagcagtcgg aggtcaatca
     4381 tcaaggtacc ggcgtgacgt aatggaatca cgagggtttc cttagccccg ggggcgagcg
     4441 gcggtcgcgc tgcgagcggt tcggctgcag cgacgccctc aatcccatgc cagttgagta
     4501 ctgtcggcac cggtaggtcg ttcaaaagcg tgatctcaag cgcatcaccg cgcttcaggc
     4561 cgatatcgcg gtctggcgct ggccccccga gcgaccatat cggtgtatcc ggccgcgctg
     4621 gacgcagggc gatgacatca gcaccggccc gcagggtcag ggagggactt tggcgctggg
     4681 cggtcgcgat cgaaggcatc gccggactga ggaccacggc gcccaatccg gctatgacat
     4741 cacgtcggtc gagcgggaaa atactatctg ccataaggtc gatgcggacc attttccgct
     4801 ggatatgtcc agttgccagg gggcctgagg agaagctttt gaaccggcca tttttttgct
     4861 gcgaacaggt cggcccatgt tataagcccg ccgcccgcgg catcgcggtc ggacatgatg
     4921 ctattctagc gggcgtggcg gaactggtag acgcgctgga tttaggttcc agtgacgaaa
     4981 gttgtggggg ttcgagtccc tccgcccgca ccaagcgctg acagcgtttg catgatgaat
     5041 actggaggac ctatctcttg aagggccggt tgcccgaaga aatggggtcc gccgaaccac
     5101 cggcgcaggc tttggggtcg cgcgtcgacc agaattgaca atgtccgggc cgttcaggcc
     5161 ggaccaagcg ggaagaagat tgacgccatg caggtcaccg aaaccctcgc tgagggattg
     5221 aagcacgaat tccagatcag cgttcccgca tcggatctcg atgccaaggc ggacgtccgt
     5281 ttcgtggaac tcaaggacaa ggtccgtctc aacggctttc gtccaggcaa ggtgccggtc
     5341 agccacctga agaaggtata tggccgctcg gtgatggcgg agaccatcga tcagaccatc
     5401 cgcgatacca actcccagat cttcaccgag cgcggcttcc ggctggcgac cgaacccaag
     5461 gtcacgatgc ccacggagca aaaagccgtc gaggacatcc tcaccggaaa atcggatctc
     5521 acctataccg tttcgatcga ggtcgtgccc acgattgcgc tcgccgactt caagagtttt
     5581 gcggtcgaga aaccggtggt cgaggtgacc ggtgccgacg ttgacgaggc catcaaacgc
     5641 atcgccgacc agaatcgcag cttcgccgcc aagggcgacg gcgcaaaggc cgagaacggc
     5701 gaccgggtca cgatctcctt caagggcacc atcgacggcg cgccattcga cggcggcacc
     5761 ggcgagaaca tccaggtgac gatcggctca aacaccttca ttcccggttt cgaagaacaa
     5821 ctgctcggca tcggggcggg cgaaacccgc gtcttgaaag tatcgtttcc gaagaactac
     5881 gccagcgaga agctcgccgg ccagccggcc gagtttgaga ccaccgcgac gttgatcgag
     5941 gcgccgcagg agactgcgat caacgacgag ttcgccaaga ccctcggtct cgaatcgctc
     6001 gacaagctca aggaagcggc gcgcgagcgg cttgtggcgg aattcgccgg cgcaacccgg
     6061 cagcgggtca agcgcatgct gctggaccgc ctcgacgaga gccatcgttt cgaggcgccg
     6121 ccgtcgctgg tcgaggaaga attcaacctg atgtggaact cgatcaaggc cgaaatggaa
     6181 tccggcggca agacgtttgc cgatgaggac accacggaag aggccgccag ggaagaatac
     6241 cacaagatcg ccgatcgtcg ggttcggctc ggtctggtgc tgtccgagat cggcgaaaag
     6301 aacaagatca cggtcaccga cgacgaggtc agccgcgcgg tcatcgagcg cgcgcgccag
     6361 atgcccggtc acgagaagga ggtctgggac tactatcgca acaataacaa cgcgctggcc
     6421 cagcttcgtg cgccgattta tgaagacaag gtggtcgatt tcatccttga actcgccaac
     6481 gtcacggaaa agaaggtcac gcgcgaggat ctctacaagg acgatgaggc cgagaagagc
     6541 gctgcctgac gacatcggcc cggcgttaag gccatgcggc gaaacgggca tagcggcagt
     6601 atccgccgct atgcgctggc gtaagaatca gcttgaactg gaatgacttc gcgcgatgtt
     6661 ggcttggtat tgacttggcc ttgtcgccgg ggaattggct catatctgtg agccctgtct
     6721 tttaactcct gtaactcctg catcacggaa cgttcgtccg ccgcccggca aattctagcc
     6781 agcccagtct tgctagtttt cttgccgatg gatgttcgcc tccgccaacc cttgggtgac
     6841 taatgcgcga tccggtggaa acctacatgc aactcgtgcc gatggtggtt gaacagacca
     6901 accgcggcga acgggcctac gatatttttt cccgcctgct gaaagagcgc atcatcttcg
     6961 tgaccggacc cgtcgaggac ggcatgtcga ccttggtggt cgcgcaactg ctgtttctcg
     7021 aagccgataa tccgaagaag gaaatctcga tgtacatcaa ctcccccggt ggagtggtga
     7081 cctcgggtct tgcgatctac gacaccatgc agttcatccg tccggcggtg tcgaccctgt
     7141 gcaccggaca ggcggcgtcg atgggttcgc tgctgctggc cgcgggccag aaggatatgc
     7201 gcttctcgtt gccgaattcc cgcatcatgg tgcaccagcc ctccggcggt tttcagggcc
     7261 aggcgaccga catcatgttg cacgcgcagg agatcctgaa cctcaagaaa cggctcaacg
     7321 agatttacgt gaaacataca ggccagacct tcaaggcgat cgaggatgcg ctggaacgcg
     7381 acaagttcct taccgccgaa atggcccggg acttcggtat tgtcgacaag gtgatcgaca
     7441 aacgctcgga agacccgatg gcgaagagcc cgtagcacca ccccacgggc caaaaaggtt
     7501 gcgttggcgt taataatcgc cgtaaccggc agttttgacc gcgaaccgtg ccgttatgcc
     7561 gcggaagttc cggtttcatg ctggtttggc agcgtgacaa tcgcgcaacg ccgggctgat
     7621 ttcggcatct gggctcgcct ggacggtgca aatcacggta ttgtcacggg tctcgggtgt
     7681 tcgcccagtt agcgaattct tgatagtcgg ctgtcagcat ggttggctag gtcggatcgg
     7741 ttatgatcgt gggggattcg agctggctgc gattcgcgtg gctgctggag agtagggtct
     7801 tttttggtac ggattttgct ctatctacac ctctgtgccg gtgcgtgccg gggtggggtg
     7861 atcgagcgga cggacggaga taagaatgag taaggtcggc acgagcgact ccaagaacac
     7921 gctgtattgc tcgttctgcg gcaagagcca gcacgaagtc cgcaaactga tcgcgggtcc
     7981 cacggtattc atctgcgacg agtgcgttga gttgtgcatg gatatcatcc gcgaggaaaa
     8041 caagtcttcg ctggtgaagt cgcgcgacgg cattccgacc cccaaggaaa tctgcaaggt
     8101 tctcgacgat tacgtgatcg gtcagagcca cgccaagaag gtgctctcgg tcgccgttca
     8161 caatcactac aagcgcctca atcaccagac caagcacaac gatgtcgagc ttgcgaagtc
     8221 gaatatcctg ctgatcggtc cgaccggttc gggcaagacg ctgctggcgc agacgctggc
     8281 gcggattctc gacgtaccgt tcacgatggc ggatgcgacc acgctgaccg aagccggcta
     8341 tgtcggcgag gacgtcgaaa acatcatcct caagctgttg cagtcggccg actataatgt
     8401 cgagcgcgcg cagcgcggaa tcgtctacat cgacgaaatc gacaagatca gccgtaaatc
     8461 cgacaacccg tcgatcaccc gggatgtgtc gggcgagggt gtgcagcagg cgctgctgaa
     8521 gatcatggaa ggcacggttg cctcggttcc tccgcaaggc ggccgcaagc atccgcagca
     8581 ggagttcctg caggtcgaca ccacgaatat tctgttcatc tgcggcggtg cgttttccgg
     8641 cctggaaaag atcatctccg cgcgcggacg ctcgacgtcg atcggtttcg cggctcaagt
     8701 gctggcgccg gaagatcgcc gcaccggcga gatcttccgt cacgtcgagc ccgaggatct
     8761 gttgaagtac ggcctgattc cggagttcgt cggccgcctg ccggtggtcg ccacgctgga
     8821 ggatctcgat gaagcttcgc tcaagaagat cttgaccgat ccgaagaacg cgctggtgaa
     8881 gcaatatcag cggctgttcg agatggagaa catcgagctg acgttcgccg atgaggcgct
     8941 tggcgcagtc gcccgcaagg cgatcgagcg caagaccggc gcgcgcggct tgcgttcgat
     9001 tctcgaaagc atcctgctcg aaaccatgtt cgatttgccg ggcctggaag gcgtcgaaga
     9061 ggtcgtgatc tcgcgcgaag tggtcgaggg taccgcccgc ccgctctata tctacgccga
     9121 tcgttccgac cgcgccgtcg aaaccagcgc aagcgcctga tcgcgcgggc caactgcggt
     9181 ttaagtatcc aaaagagacg gctgccgagc tcttcggcgg ccgtagttgc gtagtcatcc
     9241 ccacgcgttc agccgcattt cgcgggcctt ttgcagcgac ttgacacctc tgtggtcgat
     9301 agccacgtaa tagtatcggc gacgaacact tggctgtaag attcgccgca acaacgatcc
     9361 ggtgaggttg accccaagca ggccatacga tccggaacct ctcggcacct tgtggcggtt
     9421 gcgcaaaatg gcggactgcg tgcaaggggg cacaacaaaa ggaataggcc atgaccaccc
     9481 cgaaaccccg gccatctatc gttcacggcg aaagccattc ttatccggtg ctgccgcttc
     9541 gcgacatcgt cgtttttccg catatgattg tgccgctgtt cgtcggccgc gagaaatcga
     9601 tccgcgcgct cgaagaggtc atgaagaatg acgccttgat catgctggcg acgcagaaga
     9661 acgcgtcgga cgacgatccg gccccggact cgatctatga aaccggcacg cttgccagcg
     9721 tgctgcagct gctcaaattg ccggacggca ccgtgaaggt gctggtggaa gggctggagc
     9781 gcgcccgcgt gcaaaaatat tccgatcgcg ccgaatatta cgaagcggcc gcgatcgcac
     9841 tcgccgatac cgacgccacc tcggtcgagg cggaagccct ggcgcgctcg gtggtgtccg
     9901 acttcgagag ttatgtgaag ctgaacaaga agatctccgc cgaagtcgtc ggcgtggttc
     9961 aggccatcac
//
LOCUS       DATNOI010000153         7177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_686556,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000153 DATNOI010000000
VERSION     DATNOI010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686556"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1233
                     /locus_tag="VN968_05830"
                     /pseudo
     CDS             19..1233
                     /locus_tag="VN968_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            complement(1242..1940)
                     /locus_tag="VN968_05835"
     CDS             complement(1242..1940)
                     /locus_tag="VN968_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28792.1"
                     /translation="MNFKWVGPLALLAVLVIGDQIRINRPGHKYRLTVEVQTPEGVKS
                     ASGVMAVYPDRSYSRGGHTLTRGDAVLVDLGGGKNLLALLAHIDNKSVDLDGMNYVAL
                     RAYNAAGRKASFNEMSRMTGAVPVKGTLVPVLVTFTDLNDPGTAREVLPDEVEAGLGK
                     GFHVGSISVEVVPNGLWPLDFGGRLGEPVTRGIEAKLSWWNAADNPAARALQAAGLQT
                     GGAIDPKEVFTRKQ"
     gene            2091..3218
                     /locus_tag="VN968_05840"
     CDS             2091..3218
                     /locus_tag="VN968_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXL28793.1"
                     /translation="MTISADHRHAARNDLAWAISVGGIGVVAFAALLVFAWHFAATLF
                     LIFAGMLLGVALNAVTNMLGRVVNLAHSLRLTIVCLVLAGLLSGVVFLGGATIAEQAT
                     ALSGTIKSQLVGVKAFLDRNGVDTSYFELGNLAATPTDSSTPATSGAPATHNLPSAGA
                     IASGGGAIVSQTLKLLLGTVSAVGNFFIVLFLGLTFAAQPSVYRKGLLFMAPIRHRAR
                     ATAIVDRISETLERWLIAQIITMFAVFVVTWIGLAIIGVQSSFILGIQAGLLAFIPTV
                     GALLAGLIVVLASLASGWVAAASAFALFLGVHAMESYILTPIIQRHALDIPPATLFAF
                     QILLGVVFGIWGLALALPLMAIAKVMIDHFKADETPEALAA"
     gene            complement(3239..3541)
                     /locus_tag="VN968_05845"
     CDS             complement(3239..3541)
                     /locus_tag="VN968_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HXL28794.1"
                     /translation="MITEIAQIDVKPGTEKDFEAAVAKARPLFLRAKGGKGFELHKSI
                     EKPSRYRLMAKWDTLENHTVDFRGSEDFTAWRGLVGQYFASPPEVEHTETVLTSAG"
     gene            complement(3662..3895)
                     /locus_tag="VN968_05850"
     CDS             complement(3662..3895)
                     /locus_tag="VN968_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28795.1"
                     /translation="MYRPRSQSRHAALTSLEAAAASARGGFACMFSTSDEYETALIME
                     RRAQGRYRRRSPWPAVMFVGCALMVAGTVCLLS"
     gene            complement(3955..5181)
                     /locus_tag="VN968_05855"
     CDS             complement(3955..5181)
                     /locus_tag="VN968_05855"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXL28796.1"
                     /translation="MSLDTTTLYFVATMVAAMLGAMLLFFGKQEQIPALNWWGTAYLL
                     GAASIALWTLAGSALGAMLSLALNALGFVACGMVWNAARVFHGRKPNLPGLLLGAVAW
                     VAAVMTLQPEASTIRLTIGAGIVAIYAALTAAELWSERRKTMQKRWPAIAVPVLHGFV
                     LMLPILLGGLLHPHDNAFASSVWVTVFAIELVLYAVGTVFVIFMLVSERTVTAHKTAA
                     SMDPLTGLFNRRGFAEATSRVIEREANAGRPVTVLIFDLDHFKSINDRFGHPAGDEIL
                     KLFATVVINTLRITDLSGRIGGEEFAALLPCPLEEGVIAAERVRETFAASGIVAEEGP
                     VDTSVSIGVAGGPAGTELEVLLAAADSALYQAKRNGRNRVEAAEELPLSLEKWRRKTA
                     GLPGHAQRQPGVPRPA"
     gene            complement(5416..5892)
                     /gene="rpsI"
                     /locus_tag="VN968_05860"
     CDS             complement(5416..5892)
                     /gene="rpsI"
                     /locus_tag="VN968_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HXL28797.1"
                     /translation="MSDTMQSLDQLSQLKPAAPEAPKYVKKVDKLGRAYATGKRKDAV
                     ARVWIKPGAGKVTVNTREVEVYFARPVLRMMIQQPLVAAARAGQYDVICTVAGGGLSG
                     QAGAVRHGISKALTYFEPDLRGVLKKGGFLTRDSRVVERKKYGRAKARKSFQFSKR"
     gene            complement(5894..6358)
                     /gene="rplM"
                     /locus_tag="VN968_05865"
     CDS             complement(5894..6358)
                     /gene="rplM"
                     /locus_tag="VN968_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HXL28798.1"
                     /translation="MKTFSAKPAEVTKKWVLIDAKGLVVGRLASLVAMRLRGKHLPIY
                     TPHVDCGDHVIIINAAHVVLTGRKRDNKVYYNHTGFIGGIKERTAKSILEGRFPERVI
                     EKAVERMIPRGPLGRVQFGNLRVYPGPEHPHEAQQPETVDIASMNRKNMRAA"
     gene            complement(6514..6945)
                     /locus_tag="VN968_05870"
     CDS             complement(6514..6945)
                     /locus_tag="VN968_05870"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXL28799.1"
                     /translation="MATAKMSVAELERFLHVEFPQVFGDGGDISIEGADGASCLLRQR
                     YNDEMLRPGGTVSGPTLMALADYAMYVVLLSAIGPAGLAVTTNFNINFLRKGAPGQDV
                     LAAARLLKLGKRLAVGEVNLLSGSSPDPIAHVTATYSIPNA"
     gene            7019..>7177
                     /locus_tag="VN968_05875"
     CDS             7019..>7177
                     /locus_tag="VN968_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611034.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXL28800.1"
                     /translation="MSAQTARIPTRHASILLRETLGSVSVLTLNRPEARNSLSEGLIA
                     ELHAALKDI"
BASE COUNT         1334 a         2331 c         2127 g         1385 t
ORIGIN      
        1 tttgccggat gtttcgcgct gcccggactt gtcgacatgc atgtccattt gccgcccgac
       61 aatgccctga agcttaccca gggtgcagcg ctcctctacc tcttgcacgg cgtgaccagc
      121 atgcgcgaag ccggcgacct cgatggaaca gccgtcgccg cggcgcgaag attaagcagc
      181 gaagcagcgc atccggtgcc gcgggtgttc tcctgcggtc cctttgtcgg cgccggcagg
      241 gcaactttca agaacacgat cctgctggag gacgcgagcc cggcggcggc cgatgctgcc
      301 gcacgccggg tcaaggctga cggcgcgagc ttcatgaaat tctacgaggg tttgaccgag
      361 ccgatgatcc ggtccctgga gcaggcatgc gccaagcacg gcctcaaaat gatgggtcac
      421 gtccccgccg gcatcactta cgaggacgcg cgcatttccg aggtgcagca tttcttcggc
      481 gtgcccgagc cacagacgct ggaacgcgac acgctcgtca accgctcgtg cgactgacac
      541 gcggtcgacg aacggcggat ggataggatc gtcgaagcaa cgctggcgca cggcattgcc
      601 aatacgccga ccatcgtcac caaccaaaag atgctctgtt atcgggactt tgacgcagcg
      661 cgccggcaat ctgacatgcc aagggttccg ccattctatc tcgacgtgat ctggcatccc
      721 gagcgtggcc gattcaacaa ccggctgccg cgcgactatc tggagcggca ggtggttcca
      781 gcgatcgcaa gaaagcagcg gctgacgagg aaacttttcg atgccggcgc gcagctatat
      841 ctcggaaccg atgtcgcaca acccttcgtc atcccgggcg tcagcctgct cgatgaaatg
      901 gcgctgtttg tcgaggccgg gatcggcgtt gagcaagtct ggaagctcgc gacatcagac
      961 gcgggcgacc ggttcggcct gcgcggcctc ggccgcgtcg aaaccgatgc gccagctgac
     1021 attttattgt ttcggcgcga tccgaccgag gcgatctcca acatttcgag cctggaggcc
     1081 gtggtcgcgg cgggcaagct atatcgcgtc gccgatctca atcgcgccct ccaatcgagc
     1141 gaggcgtatt tcacctcgcc gctgatcaag ccgctcgccc ggcgcggcac cgagcgcgcg
     1201 ctggcgcgcg cattcagccg cactcagggg taatttcgcg gtcattgttt tcgcgtgaag
     1261 acctcttttg gatcgatcgc accgccggtc tgcagtcccg ccgcctgaag ggccctcgcc
     1321 gccgggttgt ccgcagcatt ccaccacgac aatttcgcct cgatcccgcg tgtcaccggc
     1381 tcgccgagcc gaccgccgaa gtcgagcggc cacaaaccgt tcggcaccac ctccaccgaa
     1441 atcgagccga catgaaagcc tttccccaac cccgcctcca cttcatccgg cagtacctcg
     1501 cgcgcggtcc cgggatcgtt cagatccgtg aaagtgacga gaaccgggac cagcgtgcct
     1561 ttcacgggca ccgcaccggt catccggctc atctcgttga atgacgcctt gcggccggcc
     1621 gcgttgtagg cgcgcaacgc cacatagttc atgccgtcga gatcgaccga tttgttgtcg
     1681 atatgcgcca gcaatgcgag gaggtttttt ccaccgccga gatcgaccaa tacggcatcg
     1741 cccctggtga gggtgtgccc gccacggctg tagcttctgt cgggataaac cgccatgacc
     1801 ccggaggccg atttgacccc ttcgggcgtt tgcacctcta ccgtcaaacg atatttatga
     1861 ccgggccggt tgatgcgaat ctggtcgccg atcacaagca cggccaataa agcaagcggc
     1921 ccgacccatt tgaaattcat gcttgcagcc tctcgccacg ccgcgatccc attcattgat
     1981 cgcttcgcac aaccaacatg tcagcgtcaa accggcgtgc cttctccgct tgatcccggg
     2041 cgcatttgac gaaagagtgg tgtggagaat taacctgaca aggaatcaat gtgaccatct
     2101 cggctgacca tcgccatgcg gcccgcaacg atctggcgtg ggcgatatcg gttggcggca
     2161 tcggcgtcgt cgcatttgcc gctttgttgg ttttcgcctg gcatttcgcc gcgacgctgt
     2221 ttctgatctt tgccggaatg ctgctcggtg tcgcgctcaa cgccgtgacc aacatgctgg
     2281 gtcgcgtcgt caacctggcg cattcgctgc ggctaacaat tgtctgcctg gtgctcgccg
     2341 gcctgctgtc gggcgtggtc tttctcggtg gcgccaccat cgcggaacag gccacggcgc
     2401 tgagcggcac gatcaagtcg caattggtcg gcgtgaaggc gttcctcgac agaaacgggg
     2461 tcgacaccag ctatttcgag ctcggcaatc tggccgcgac ccctacggac tcatcgacac
     2521 cggcaacatc aggtgcgccg gcgacacata atcttcccag cgccggcgcg atcgcttcag
     2581 gcgggggcgc gatcgtgagc cagaccctga aattgcttct gggtacggtg agcgctgttg
     2641 gaaatttctt tattgtgctg tttctcggac taacgttcgc agcgcagccg agcgtctacc
     2701 gaaaggggct gttgttcatg gcgccaattc ggcaccgcgc acgcgcgacc gcgatcgtcg
     2761 accgtatcag cgaaacgttg gagcggtggc tgattgcgca gattatcacc atgttcgcgg
     2821 tcttcgtcgt gacctggatt gggctggcga tcatcggcgt ccagagctcc ttcattctgg
     2881 gcatccaggc cggactgctc gccttcattc cgacggtcgg cgcgctgctc gccgggttga
     2941 tcgtggtgct tgcgagcctc gcatccggtt gggtcgccgc agccagcgca ttcgcgctgt
     3001 tcctcggcgt tcacgcgatg gaaagctaca ttctcacacc gatcatccag cgccacgcgc
     3061 tcgacattcc cccagcgacg ctgttcgcgt tccagatcct gctcggcgtc gtgttcggta
     3121 tctggggttt ggcgctggct ttgccgttga tggcgatcgc gaaagtcatg atcgatcatt
     3181 tcaaggctga cgaaacgccc gaggcgctgg cggcctgagg ccgcccgtca tcggttaatc
     3241 agccggccga ggtcagcacc gtctcggtgt gttcgacttc cggtggcgac gcaaaatact
     3301 gcccgaccaa cccgcgccag gccgtgaaat cctccgaacc cctgaaatcg acggtgtgat
     3361 tttccagcgt gtcccatttt gccatcagcc ggtagcgtga cggcttctcg atcgacttgt
     3421 gcagctcaaa acccttgccg cccttggcgc gcaggaacag cgggcgagcc ttggcgaccg
     3481 cggcctcaaa gtctttctcg gtgccgggct tgacgtcgat ttgtgcgatt tcggtgatca
     3541 ttgggttctc ggccttcggt tgagaattca catccctagc ccagccggcc gcattattga
     3601 agtcacggaa cgtccaaaag cggcgcctgg cggtgcccgc atccccactt cccgaggctc
     3661 ctcagctcaa gagacacacc gtgcccgcca ccatcagcgc gcaaccgacg aacatgacgg
     3721 ctggccaagg gcttcgccgc cgatagcggc cctgagcgcg ccgttccatg atcagcgccg
     3781 tctcatattc gtccgacgtc gagaacatgc aggcaaaccc gccgcgcgcc gatgcggctg
     3841 cggcttcaag cgaggtcagc gccgcgtgac ggctttggct gcggggcctg tacatggcct
     3901 ccgatgatat ggaccgaacg gtaaacgaga ggttactgcc gtcgcgccac cgctttatgc
     3961 gggacggggg acacccggct gccgttgcgc gtgacccggg aggcccgccg tttttcgccg
     4021 ccatttttcc agcgacaacg gcagctcttc tgctgcctcg acgcggttgc ggccatttcg
     4081 cttggcctga taaagcgcgc tgtcggcggc cgcgagcagc acctcgagtt cggtgccggc
     4141 cgggccgccc gccacaccga tgctgaccga cgtgtcgacc ggtccctcct cggccacgat
     4201 tccggaggct gcgaacgtct cgcgcacgcg ttcggcggcg atcacacctt cctccagcgg
     4261 acagggcaac aacgcggcga actcctcgcc accgattcgt cccgagaggt cggtgatccg
     4321 cagcgtgttg atcaccaccg tggcaaacag tttcaggatc tcgtcgcccg ccgggtggcc
     4381 gaagcggtcg ttgatcgact tgaaatggtc aaggtcgaag atcagcaccg tcaccggacg
     4441 gccggcgttg gcttcgcgct cgatcacccg tgacgtcgcc tccgcaaagc cgcggcgatt
     4501 gaacaggccg gtcagcggat ccatcgacgc cgccgtcttg tgcgcggtca ccgtgcgctc
     4561 cgacaccagc atgaagatca cgaaaaccgt gccgactgca tagagcacaa gctcgatcgc
     4621 gaatacggtc acccagacgc tgctcgcgaa cgcgttgtca tgcgggtgca gcaggccgcc
     4681 gagcaggatc ggcagcatca gcacgaaacc gtgcagcacc ggcactgcga tcgccggcca
     4741 gcgcttctgc atcgtcttgc ggcgctcgga ccacaattcg gcagccgtca gcgccgcata
     4801 tatcgcaacg atgccggcgc cgatcgtcag gcgtatcgtc gacgcctccg gctgcagcgt
     4861 catgacagca gcgacccagg ccacggctcc gagcaggagc cccggcagat tgggcttgcg
     4921 gccgtggaac acgcgcgctg cattccagac catgccgcag gccacgaaac cgagtgcatt
     4981 cagcgccagc gacagcatcg cgccaagcgc ggaaccggcg agtgtccaga gcgcgatcga
     5041 cgcggcgccg agcagatagg cggttcccca ccagttcagt gccgggatct gctcctgttt
     5101 gccgaaaaac agcagcatgg ctcccagcat tgccgcaacc atcgtggcaa cgaaataaag
     5161 cgtcgtggta tcgagcgaca tggaccaccg aataagattg caatgcagcg tatccggcgc
     5221 gcttgaaaaa gggcaccacg ccttggtctt ttcagatcta cgtcgaccgc gtttgaaatc
     5281 cctttgtttg cacgcgcaag ttttccggaa aaactgcttc aactcttcgg caaatgatcg
     5341 acaaaaaaag gcgcccgaag gcgccctttg aagtttcatc tctgctggat tcgcagcggg
     5401 atagtatcgg tgcggctacc gcttcgagaa ctggaacgac ttgcgcgcct tggcgcggcc
     5461 gtacttcttg cgctcgacca cgcgcgagtc gcgggtgaga aagccgccct ttttcagaac
     5521 gccgcgcaga tccggctcga aataggtcag cgccttcgag atgccgtgac gcaccgcgcc
     5581 ggcctggccc gacagaccgc caccggccac ggtgcagatc acgtcgtact gccccgcgcg
     5641 ggctgccgcg accaacggct gctggatcat catccgcagc accggccgtg caaagtagac
     5701 ttcgacctcg cgggtattga cggtgacctt gccggcgccc ggcttgatcc aaacgcgggc
     5761 caccgcgtcc ttgcgcttgc cggtggcgta ggcgcggccg agcttgtcga ccttcttgac
     5821 atatttcggc gcttccggcg cggccggctt cagttgcgac aactgatcga gcgactgcat
     5881 ggtgtcggac atcttatgcg gccctcatgt tcttgcgatt catcgaagcg atgtccaccg
     5941 tctcgggttg ctgggcctcg tgcggatgct cgggcccggg gtaaacgcgc aagttgccga
     6001 actgcacccg gccaagcggt ccgcgcggaa tcatgcgctc gacggccttc tcgatcacgc
     6061 gctcggggaa acggccctcg aggatcgact tcgccgtgcg ttccttgatg ccgccgatga
     6121 agccggtgtg attgtaatag accttgttat cgcgcttgcg cccggtgagc accacgtgag
     6181 ccgcgttgat gatgatgacg tggtcaccgc aatcgacgtg gggcgtatag atcgggaggt
     6241 gtttgccgcg aaggcgcatc gcgacaagtg aggcgagccg tccgaccacc agacctttgg
     6301 cgtcgatcag cacccacttc ttcgtcacct cggctggctt tgccgaaaac gttttcatgt
     6361 gaggaaatcc gtgaaagggg aacatcggac gcatgcgcgc cgaactgcgc ggtttctaga
     6421 taggtccgcc gcgcgcgtca atgcccagct aagataatta tctcaataga atcatcggct
     6481 tataaatatg gtgctatatt accatgtaaa aggctaagcg tttggaatgg aataggtcgc
     6541 agttacatgg gcgatcggat cgggtgaact ccctgaaagc agattgacct cacccaccgc
     6601 aagccgcttg cccagtttca aaagtcgcgc tgccgcaagg acatcctgcc ccggcgcccc
     6661 tttacgcaag aaattgatgt tgaaattggt cgtcaccgca agcccggccg ggccgatcgc
     6721 cgacagcagc accacataca tcgcgtaatc cgccagcgcc atcagcgtcg gtccggagac
     6781 cgtgccgccc ggacgaagca tctcgtcatt gtagcgctga cggagcaggc aggaagcacc
     6841 atcagcgcct tcgatcgata tatcgccgcc gtcgccgaaa acctggggaa attcgacatg
     6901 gagaaacctc tccagttccg ccacgctcat cttcgccgtt gccatgtcgt tccgtccgcc
     6961 ctcgcctaac gctgtcagtt acattaagtt gtccgcccag ccctagtgga aatttccgat
     7021 gtccgcccaa accgcccgca ttccgacgcg acacgcatcg atcctcctgc gcgagacgct
     7081 cggcagcgtc agtgtgctca cgctcaatcg gcccgaagcc cgcaacagcc tgtcggaagg
     7141 gctgatcgcg gaactgcacg cggctctcaa ggacatt
//
LOCUS       DATNOI010000154         5580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_714324,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000154 DATNOI010000000
VERSION     DATNOI010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714324"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1159
                     /locus_tag="VN968_05880"
     CDS             11..1159
                     /locus_tag="VN968_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HXL28801.1"
                     /translation="MSIKGKAYIAGIYEHPTRHAPDKSIAQLHAEVARGAIEDAGLTN
                     GDIDGYFCAGDAPGGAWPMVDYLGLKVRHIDSTETGGCSYLIHLGHAAEAIAAGKCSI
                     ALITLAGKPRTGAMPPRAAGAEADFEAAYGATTHNAYGMCAMRHMHDYGTTSEQLAWI
                     KVAASHHAQYNPHAMLREVVTVEDVLNSPMISDPLHRLDCCVISDGGGALIVTTPEIA
                     KSLKKPLVRLIGHGEAMKGPRGGKDLDLTYSAGIWSGPRAFEEAGIAPRDIKYASIYD
                     SFTITVLMQIEDLGFCKKGEGGKFVADGNLISGVGKLPFNTDGGGLCSNHPVNRGGMT
                     KIIEAVRQLRGEAHPKVQVKNCDLAIAHGTGGLLGVRHAASTCILERV"
     gene            1159..1548
                     /locus_tag="VN968_05885"
     CDS             1159..1548
                     /locus_tag="VN968_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HXL28802.1"
                     /translation="MAEPKKYPAPQGNPETAPFWEAARQGKFMIKRCTACGEPHYFPR
                     SICPFCFSDKTVWEEASGEATIYTYSLMRKSATGPYAIAYVTLKEGPSLQTNIIDCDL
                     EKLKIGQKVKVVFKPTDGAPLPFFTPV"
     gene            1684..2544
                     /locus_tag="VN968_05890"
     CDS             1684..2544
                     /locus_tag="VN968_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HXL28803.1"
                     /translation="MPINYDELMALKNLGQKYAYSDREVMLYAYGIGMGADPMDEREL
                     AFVNEAVATPRPLKVVPTFASVAAWGAGPGEMNLNRVMVVDGERDITFHKPLATAAHI
                     TADSSVLDVFDKGKDKGAVIRHQTVLKDEKGEKLATLVASRFARGDGGFGGPSEGQPE
                     PHNVPTRAPDRTIDISTRPDQALVYRLCGDRNPLHSDPEFAKRAGFPKPILHGMCTYG
                     ITCRGILRTYADYDPSAFKQHAARFSSPVYPGETVTMELWKDGNVISFEAKVKARGAT
                     VIKSGKTVLG"
     gene            2746..3624
                     /locus_tag="VN968_05895"
     CDS             2746..3624
                     /locus_tag="VN968_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL28804.1"
                     /translation="MGLLDGKVALITGAGGGLGEAYAKLFAREGAAVVVNDLGGPRDG
                     SGGDVSMAQKVVDAIKAAGGRAVANGADISTMAGGQSVFDDAIKHFGRADILVNNAGI
                     LIDQTFAKATEAAWDKVLKVHLKGTFCVSQPVFRWMRENGGGVIVNTSSTSGLIGNFG
                     QTNYGAAKGGIWGLSNVLAIEGRKYNIRVWTLAPGALTRMTADLPRYIENPGAALGPD
                     GIAPAVLYMVSELSGDQTGKVLGVSGPRGVREMRMMEKPGWKPPHQGWKAQDIVEHAR
                     EIFFSEEEIKMGARRF"
     gene            3807..4763
                     /locus_tag="VN968_05900"
     CDS             3807..4763
                     /locus_tag="VN968_05900"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXL28805.1"
                     /translation="MTKLTHKAAGTFAIAPTPFHDDGRIDDKSIDRLTDFYAEVGCDG
                     VTVLGILGEAPKLEGSEAEQVARRFVKRAKNMQIIVGISAPGFAAMRSLARSSMDAGA
                     AGVMIAPPPHLRTDEQIIGYFRQAAEAIGTDIPWVLQDYPLTLTVIMTPAVIRKIVMD
                     NPSCVMLKHEDWPGLEKISALRAFQKDGSLRPLSILTGNGALFLDFEMERGADGAMTG
                     YAFPELLIDVVRLSKEGRRDAAHDLFDAHLPLVRYEQQPGAGLAVRKYVLQKRGVIAS
                     SAQRKPGSNITATAKAEVDYLLSRVARVDKRANLQPQSSAAG"
     gene            4768..5565
                     /locus_tag="VN968_05905"
     CDS             4768..5565
                     /locus_tag="VN968_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXL28806.1"
                     /translation="MTEPVTPRPASTILLLRDAAGKDEIEVFMMVRHYEIDFNSGALV
                     FPGGSVDKGDQEIIARPELYSGGGGLDASALSFRIAAIRETFEESGILLARPHGSGAL
                     IDARRAGEIEAAHRAALCEGKTTFLQVLADNGLLLALDELVPYAHWITPEGMPKRFDT
                     WFFLAAAPPEQAGAHDGKESTDSIWVSPREALAGGESGRFKLPFPTTRNLIKLGRQKS
                     VKTALDESKGKPIVSVMPVMTRLNGGRQLRIPLEAGYDGEVFEVGAV"
BASE COUNT         1112 a         1683 c         1819 g          966 t
ORIGIN      
        1 gagcgccata ttgagcatca agggcaaggc ctatatcgcc gggatttacg agcatccaac
       61 ccggcatgca cccgacaaat ccatcgccca gttgcatgcc gaagtcgcca ggggcgcgat
      121 tgaagacgcc gggctgacga atggcgatat cgacggctat ttctgcgcgg gcgacgctcc
      181 gggcggtgcc tggccgatgg tcgattatct cgggctgaag gtgcgccaca tcgattccac
      241 cgagaccggc ggttgctcgt acctgatcca tctcggccat gccgccgaag cgatcgcggc
      301 cggcaaatgc tcgattgcgc tgatcacgct ggcgggcaaa ccgcgcaccg gggcgatgcc
      361 gccgcgtgcc gcgggcgccg aggccgattt cgaggcagcc tatggtgcaa ccacccataa
      421 cgcctacggc atgtgtgcca tgcgccacat gcacgactac ggcaccacca gcgagcagct
      481 cgcctggatc aaggtcgcgg cctcccatca cgcgcaatac aatccgcacg cgatgctgcg
      541 cgaggtcgtc accgtcgagg acgtgctcaa ttcgccgatg atttccgatc cgctgcatcg
      601 gctggactgc tgcgtcatca gcgatggcgg cggcgcgctg atcgtgacca cgcccgaaat
      661 cgccaaaagc ctgaaaaagc cgctggtgcg cctgattggg cacggcgaag cgatgaaggg
      721 acctcgcggc ggcaaggacc tcgacctgac ttattccgcc gggatctggt ccgggccgcg
      781 cgcgttcgag gaggcgggta tcgcgccaag ggacatcaaa tatgcctcga tctatgacag
      841 cttcaccatc acggtattga tgcagatcga agacctcggc ttctgcaaga agggcgaggg
      901 cggcaagttc gttgccgacg gcaatttgat ctccggcgtc ggcaagctgc cattcaacac
      961 cgacggcggc gggctgtgca gcaatcaccc cgtcaaccgc ggcggcatga caaaaatcat
     1021 cgaagcagtg cgtcagctgc gcggcgaggc ccatccgaag gtgcaggtca agaactgcga
     1081 tctcgccatc gcccacggca ccggcggcct gctcggcgtg cgccacgccg cctcgacctg
     1141 cattctggag cgtgtgtaat ggcggagcca aaaaaatatc cggcgccgca aggcaatccg
     1201 gaaaccgcgc cgttctggga agccgccagg caaggcaagt tcatgatcaa gcgctgcacg
     1261 gcgtgcggcg agccgcatta ttttccgcgt tcgatctgcc cgttctgctt ttccgacaag
     1321 acggtgtggg aggaggcttc cggcgaggcc acgatctaca cgtatagcct gatgcgcaaa
     1381 tcggcgaccg ggccttatgc gatcgcctac gtcacgttga aggaaggccc gtcgctgcag
     1441 accaatatca tcgattgcga cctggagaaa ttgaagatcg gccagaaggt caaggtggtg
     1501 ttcaagccga ccgacggcgc gccgctgccg ttctttacgc cggtgtagtc agtgggcacg
     1561 aagcacgcca agcaacagct agtcatcccc gtgcaggcgg ggatccagta aacgccggcg
     1621 attactggac ccccgctttc gcagggatga cgagcggaga aaatccggga ggatgcatcg
     1681 aaaatgccga tcaactacga cgagctgatg gccctgaaaa atctcggcca gaaatacgcc
     1741 tacagcgatc gcgaggtgat gctctacgct tatggcatcg gcatgggcgc cgacccgatg
     1801 gacgaaaggg agctcgcttt cgtcaacgaa gccgtcgcca cgccgcgccc gctgaaggtg
     1861 gtgccgacct ttgcgtcggt tgcggcatgg ggcgcggggc ccggcgagat gaatctcaac
     1921 cgcgtcatgg tggtggacgg cgagcgcgac atcaccttcc acaagccgct ggcgacggcc
     1981 gcccacatca ccgccgattc cagcgtgctc gacgtcttcg acaagggcaa ggacaaggga
     2041 gcggtgatcc ggcatcagac ggtgctcaag gacgagaagg gcgagaaact cgccacactg
     2101 gtcgcatctc gcttcgcgcg tggcgacggc ggctttggcg ggccgtccga gggccagccc
     2161 gaaccgcata acgtgccgac gcgcgcgccc gacaggacca tcgacatttc aactcgtccg
     2221 gaccaggcgc tggtctatcg tctctgcggc gaccgcaatc cgctgcactc ggatcccgaa
     2281 ttcgcaaagc gcgccggctt tccgaaaccg atcctgcacg gcatgtgcac ctatggcatc
     2341 acctgccgtg gcatcttgcg gacctatgcc gattacgatc cgagtgcttt caagcagcac
     2401 gccgcgcggt tttcctcacc ggtctatccc ggcgagaccg tgaccatgga attgtggaag
     2461 gacggcaatg tgatttcatt cgaagccaag gtgaaggcgc gtggcgcgac cgtgatcaag
     2521 agcggcaaga cggtgctggg ttagagcggc gagggcggtg cgctccctcg ccccgctctt
     2581 gcggggtcga gacgagcgaa gctcgctctt agagggttgg ggtgaggggc tcttgtcaca
     2641 tcgggactcg cggagagtcc ccctcacccg aaggcttcgc cttcgacctc tccccgcaag
     2701 cggggcgagg taaggaagag gaacgatata taaggagaaa aaaccatggg attactcgac
     2761 ggcaaggtcg cgctgatcac cggcgcgggg ggcgggctcg gtgaggccta tgccaaatta
     2821 ttcgcgcgcg agggcgcggc tgtcgtcgtc aacgacctcg gcggaccacg tgatggctcc
     2881 ggcggcgatg tctcgatggc gcagaaggtg gtcgacgcca tcaaggccgc gggcggccgc
     2941 gcggtcgcca acggcgccga catctcgacc atggcgggcg ggcagtcggt gttcgacgat
     3001 gccatcaagc atttcggccg cgccgatatc ctcgtcaaca atgcgggcat cctgatcgac
     3061 cagacctttg ccaaggcgac cgaggcggcc tgggacaagg ttttgaaggt gcatttgaag
     3121 ggcacgttct gcgtcagcca gccggtattc cgctggatgc gcgagaacgg cggcggcgtc
     3181 atcgtcaata cgtcatcgac ctcaggcctg atcggcaatt tcggccagac caattatggc
     3241 gccgccaagg gcggcatttg ggggctttcc aacgtgctgg cgatcgaagg ccgcaaatac
     3301 aatatccgtg tctggacgct ggcgccgggt gcgctgaccc gcatgaccgc cgatctgccg
     3361 cgctatatcg agaatcccgg cgcagccctt ggacctgatg gcatcgcgcc ggccgtgcta
     3421 tacatggtca gcgaactgtc gggcgaccag accggcaagg ttctcggtgt ctcgggtccg
     3481 cgcggcgtgc gcgagatgcg gatgatggag aagccgggct ggaagccgcc gcatcaggga
     3541 tggaaggctc aggatatcgt cgagcacgcc agggagattt tcttctcaga ggaagagatc
     3601 aaaatgggcg cgcggcggtt ttagcgttag ccgtcattcc cgcgcaagcg tagcgcttgt
     3661 cgctgagggc gccagtgaag cgagcgaacc cggaatccat gaccacgatc gtgagtatgg
     3721 attccgggcc tgcgccaagt agcgcatcca cgatgtgcaa ttgcacatcg ggaatgacaa
     3781 gacaagttag gttgaatgag gcacagatga caaaactcac ccacaaggcc gcagggactt
     3841 tcgccattgc gccgacgccg ttccacgacg acggccgcat cgacgacaaa tccatcgatc
     3901 ggctgacgga tttttatgcc gaggtcgggt gcgacggcgt cacggtactc ggcattctgg
     3961 gtgaggcgcc gaagctcgaa ggctcggagg ccgaacaggt cgcaagacgc tttgtgaaac
     4021 gggcgaagaa catgcagatc atcgtcggca tctcggcgcc gggatttgcc gcgatgcgct
     4081 cgctggcgcg cagctcgatg gacgcgggtg ctgccggagt gatgatcgcc ccgccccctc
     4141 atttacgtac cgacgagcag atcatcggat atttcaggca ggcggcggag gcgatcggca
     4201 ccgacattcc ctgggtgctg caggattatc cgctgacgct gaccgtgatc atgacccctg
     4261 ccgtgatccg gaaaatcgtg atggacaatc cgtcctgcgt gatgctcaag cacgaggact
     4321 ggccgggact ggaaaagatt tccgcgttgc gcgccttcca gaaggacggc tcgttgcggc
     4381 cgctctcgat cctgaccggc aatggcgcgc tgtttctcga tttcgagatg gaacgcggcg
     4441 ccgacggcgc gatgaccggc tacgcttttc cggaattgct gatcgacgtg gtgagactgt
     4501 cgaaagaagg ccgtcgcgac gctgcgcacg atctgttcga tgcgcacctg ccgctggtcc
     4561 gctacgagca gcagcccggt gcgggactgg cggtgcggaa atatgtgctg cagaagcgcg
     4621 gcgtgattgc ctcgagcgct caacgcaagc cggggtccaa cattaccgcc acggcaaagg
     4681 cggaagtgga ctatctgctg tcacgggtcg cgcgggtcga caaacgcgcg aacctgcaac
     4741 cgcaatccag cgcggcaggt tgatcgcatg actgagcccg tcacaccgcg tcctgcctcc
     4801 actatcctgc tgctgcgcga cgcagcgggc aaagatgaga tcgaggtctt catgatggtg
     4861 cgccactatg agatcgattt taactccggc gctctggtgt ttcccggcgg cagtgtcgac
     4921 aagggcgatc aggagatcat cgcgagacct gaactttatt ccggtggcgg ggggctcgat
     4981 gccagcgctt taagtttccg gatcgccgcg attcgcgaaa cctttgagga gagcggtatc
     5041 ctgctggcgc ggccgcacgg gtcgggtgcc ctgatcgatg cgaggcgggc tggcgagatt
     5101 gaagctgccc atcgcgccgc cttgtgcgaa ggcaagacca cgtttctgca ggtgctcgcc
     5161 gacaacgggc tgttgctggc gctcgatgaa ctcgtgccct atgcgcactg gatcacgccg
     5221 gagggcatgc caaaacggtt cgacacctgg ttctttcttg ccgcggcacc accggaacag
     5281 gccggcgctc acgacggcaa ggaatcaacc gattcgatct gggtgtcgcc gcgcgaagcc
     5341 ttggcgggcg gcgagagcgg ccggttcaag ctgccgtttc cgaccacgcg caacctgatc
     5401 aagctcggaa ggcaaaaaag cgtcaagacc gcgctcgacg agagcaaagg caagccgatc
     5461 gtgagcgtga tgccggtgat gacgaggctc aacggcggcc gtcagcttcg catcccgctt
     5521 gaggccggct acgacggcga ggtgttcgaa gtcggcgcgg tgtgatgcac cggaacacgg
//
LOCUS       DATNOI010000155         7948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1154030,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000155 DATNOI010000000
VERSION     DATNOI010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154030"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VN968_05910"
     CDS             complement(<1..609)
                     /locus_tag="VN968_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28807.1"
                     /translation="MIAMPAFADDAPRFQVDAAWPKPLPNDWIMGQAAGVAVDAQDHV
                     WVIQRPRSLTDDEKALTLNPPQSKCCRPAPAVMEFDSEGNLIQAWGGPGEGYDWPQVE
                     HGISIDAAGFVWIGGNGDNDGQYLKFTRAGKFVLQIGKSGQQSDSNDVTRLGRPADAE
                     VDPDTNEVYIADGYFNHRVIVFDANTGAYKRQWGAYGKKPTDE"
     gene            complement(777..1496)
                     /locus_tag="VN968_05915"
     CDS             complement(777..1496)
                     /locus_tag="VN968_05915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXL28808.1"
                     /translation="MKTGNIKVKLRDGEMGAYLAIPDRTPAGGIIAIMEIWGVNETMR
                     HHAHEFAQAGFICLVPDLFWRQEGGVELSDGNPEHVRKAFDLYYDFDYDLAVRDMEDT
                     WRYLAGLQECNGKVGAVGYCLGGKLCYLMCCRSDIDCAVAYYGTYIEHHIREAKNLHR
                     PFLLHMAMKDRWVQAEVNDLLERRLSPNPLVQIHKYPNADHAFARYGGKTYSKPEADR
                     ALELTLDFFRQHLQTAEMARV"
     gene            complement(1493..2530)
                     /locus_tag="VN968_05920"
     CDS             complement(1493..2530)
                     /locus_tag="VN968_05920"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL28809.1"
                     /translation="MKIDIFNHFMPKPYLERLAALIPGHAAVTAFPRLATLCDVEARL
                     RLLDEFGDLQNVLSLANPPLELIAGPDLTPELARIANDALAEICARHTDYFPAFVAAL
                     PMNNMDAAIAEADRAIGALGARGVQIFTHVANQPLSRPEFRPIFRRMAEHDLPIWIHP
                     MRGPDFADYKTEQQSQDEVWFAFGWPYETTACMTRLIYSGIFDELPKLKIITHHYGGM
                     IPFFSGKIKLGFRQIFYGTPERNPVVEEHHLKRAPIDYFKMLYADTAVNGEPAATRCG
                     HAFFGTRFSLFATDAPFDAEQGRGLIRETIAAVEALEISRHERERIFAGNARDLLRLS
                     AEPAVKARSRA"
     gene            complement(2542..3915)
                     /locus_tag="VN968_05925"
     CDS             complement(2542..3915)
                     /locus_tag="VN968_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL28810.1"
                     /translation="MAVADEAKSAELIARLEQLPFSRWHRSFFILAFFGIMFDAADFA
                     MFGAALPPIAREFGLGPAQSGLLATVGLIGAFFGALFWGTVSDYIGRRTSFAATVGIF
                     ALFTGLVAASWNVTSLAVSRFLSNFGLGGEVPVTSTLASEFSPGRIRGAMTGNIMAAF
                     PVGLIVAALLSLLIIPTLGWRALFVVGVVPAVLLFFVRRYMPESVRYLLSKGRLAEAE
                     ATVAQIERRALGRSLTAEEIRAAPKVKPEVAVPTGVTVFQLFAPGRARTTILVWIVSF
                     GFLWASNGILFMLPTILTQRGIPLTDAIAFQLVQAIGGFFGYSSCSFLIDRYGRRPVL
                     FLYYFVGAFFHLAFAMSTGFATYVTAFLVGWVNPGVYGATSVYVGELHPTHLRATAVG
                     WFFGIGRIGSFLAPSVVGFMLAYGLGGWTLYTFALSYLIASFALIAVGVETKGRVLEE
                     ITQAKPV"
     gene            3969..4481
                     /locus_tag="VN968_05930"
     CDS             3969..4481
                     /locus_tag="VN968_05930"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXL28811.1"
                     /translation="MGFASITPGYKDIAMTSSRLLRWLSRSPVQTFILCPLLVVAFEL
                     VRQRGELTTEPAGVVLLAWGYLQYLLVGRFRTRVGGGGPGFGVPPDRIVEDGPYRYVR
                     NPMYLGHLIVMLGLAVTFVSWFALILFAARALWFHRRVLEDEARLEKMFGPAYLDYQR
                     RVKRWIPGLL"
     gene            complement(4560..5543)
                     /locus_tag="VN968_05935"
     CDS             complement(4560..5543)
                     /locus_tag="VN968_05935"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL28812.1"
                     /translation="MTRALGVGIVAALIAAAPVAAQAQDYPTRAITIVVPFAPGGLTD
                     VPVRVLSAMMQERIGQNIVIENKPGGSGVVGGTYAVRAAPDGYTLFANSIADAQNLHY
                     IPVPYNAVDDFAMIGLIVEGPPLVLIIDAKLSYKSLAELLADARANPKKISFGTSGPA
                     TSPAIALTQLNSLGKTEIVGVPYRGSGEAARNVAAGLIEGAFAFYAQAKPLADDGKVR
                     ALAVAAPQRIASWADVPTMQELGFPNFDYSGFVGLAAPAKTAPAIIAFLNKELNAVVQ
                     SRPFRERMEALGMTVPADNTPDKLADVLRRETVRQAALAKLSGHAPLAPQR"
     gene            5713..7029
                     /locus_tag="VN968_05940"
     CDS             5713..7029
                     /locus_tag="VN968_05940"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXL28813.1"
                     /translation="MSNKSALRAAEPLLPVELGAGKVKFAQGMKAGRWVFATGLMAQD
                     FRNGIAPDVLATRAPHAGLPKREKEAQRIFENLETVLSAAGTERSNLVRTDQYYTTVT
                     AVPPYQQVRREFLRGRVPPSTSITQKRLLLPDADMSVQALAVIPDKDFTVEHLKHEHL
                     KGRPTSGYSPALTVGDFIFIPGITSMAVGDEPRRNGIAAAALMAEGAQWGGQPIKLET
                     EFIITQRIVPSLKLVGAGLEDVAHAQIYLTDAEDFSAFTEVWSRHFGENGPTFSVIPC
                     IEHGLAPYHGKIEINVLAVRPGSSARKRHIDAGVTTALHRQPQALRAGDLLFISGLMA
                     ADRDGLAPSAARDPRQPWFSSPAEMQADIIIGNIKRLCEAAGTSLENVVRLQQFHTDI
                     SEFYAVYKVWEHHLAGRPLPFSAVEVPAPLPVPGASVLIEAWVYAP"
     gene            complement(7243..>7948)
                     /locus_tag="VN968_05945"
     CDS             complement(7243..>7948)
                     /locus_tag="VN968_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28814.1"
                     /translation="SMLWLPVINGSLCAVFMVVFLRLLGDVNETVLAGSLAVAVVGAW
                     ILIAFRPQVIAGIPESRQLPYAIICTLAGLLLVALVHFLLPEVHFTAADRSIFSWQSI
                     DHTILSSKVKIVLFALALALFLVATAHRRVPDGVRGVLAGLPLVPFGALVSVAALNDL
                     DTKEQQRVFENMLRSVALSPAVAIWFIYGFSRYLKSRVFVGKLDGLNRFASLILAWTL
                     SGVMIASLSWALIVAL"
BASE COUNT         1594 a         2454 c         2434 g         1466 t
ORIGIN      
        1 ttcgtcagtc ggctttttgc cataggcgcc ccactgccgt ttgtaagcgc cggtattggc
       61 gtcgaatacg atgacccggt gattgaaata tccgtcggcg atatagacct cattcgtatc
      121 cggatcgacc tccgcatcgg ctggacggcc aagacgggtc acgtcattgc tgtcgctctg
      181 ctgtcctgat ttgccgatct gcagcacaaa tttcccagcg cgcgtgaatt tcagatactg
      241 tccatcattg tctccattgc cgccgatcca gacgaaaccc gctgcatcga tgctgatgcc
      301 gtgctcgact tgcggccagt catagccctc gccggggccg ccccacgcct ggatcaaatt
      361 gccttcggag tcgaactcca tgaccgcagg cgccgggcgg cagcatttgg actgcggtgg
      421 attgagcgtc agcgccttct cgtcatcagt gagcgagcgc gggcgttgta tgacccagac
      481 atgatcctgc gcgtccaccg ccacgcccgc ggcctgaccc atgatccagt cgtttggcag
      541 cggcttgggc caggcggcgt cgacctgaaa gcggggagcg tcatcggcga aagccggcat
      601 ggcgatcatc gccgccagaa gcactgacaa aatgaacctc acggtatcct ccctgtgcga
      661 gctgtgaacg gctgtgggac gcggcgctct ctctttttcg acaggcataa ggacgaggct
      721 aagcgcgtta tcccactcga tccgcatgga atcgcggacc ttcgatttgt gcatgatcat
      781 accctcgcca tctcggccgt ctgcaggtgc tggcggaaaa aatccagcgt caactccagc
      841 gcacggtccg cttccggctt gctgtaggtc ttgccgccgt aacgcgcgaa ggcgtggtcg
      901 gcattgggat atttatggat ctgcaccagc gggttgggtg acaagcgccg ttccaacaga
      961 tcgttcacct cagcctgaac ccaccgatcc ttcatcgcca tgtgcagcag aaacgggcgg
     1021 tgcagatttt tcgcctctcg gatgtggtgt tcgatgtagg tgccgtaata ggcgacggcg
     1081 caatcgatat cgctgcggca gcacatgagg taacaaagct tgccgccgag acaatatcca
     1141 acggcgccaa ccttgccgtt gcactcctgc aatccggcaa ggtagcgcca cgtgtcctcc
     1201 atgtcgcgaa ccgcgaggtc gtaatcgaaa tcgtaataaa ggtcgaacgc cttgcgcaca
     1261 tgctctggat tgccgtcgga gagttcgacg cctccctctt gccgccagaa taggtcgggg
     1321 accagacaga tgaagccagc ttgtgcgaat tcgtgggcat ggtggcgcat ggtctcgttc
     1381 acgccccaga tttccatgat ggcgatgatc ccgcccgccg gcgtgcggtc ggggatcgcg
     1441 agataagctc ccatctcgcc gtcgcgcagc ttcactttga tgttccctgt cttcatgcgc
     1501 gcgatctcgc tttcaccgcc ggctcggcgc ttagtcgcag gaggtcgcgc gcgttgccgg
     1561 cgaagatgcg ttcgcgctcg tggcgcgaaa tctccagcgc ctcaaccgct gcgatggtct
     1621 cgcgaatgag gcccctgccc tgctcggcat cgaacggcgc atctgttgca aacagggaaa
     1681 agcgcgtgcc gaaaaacgcg tgtccgcagc gcgtcgccgc gggctcgccg ttcactgcgg
     1741 tgtcggcata gagcatcttg aagtagtcga tgggcgcgcg cttgaggtga tgctcctcga
     1801 caaccggatt gcgctccggc gtgccgtaga aaatctggcg gaaaccgagc tttatcttgc
     1861 cggagaagaa cgggatcatg ccgccgtaat gatgggtgat gatcttcagc ttcggcagtt
     1921 cgtcgaaaat tccggaatag atcaggcgcg tcatgcaggc cgtggtctcg tacggccagc
     1981 cgaaggcgaa ccagacttca tcctgcgact gttgctcggt tttgtaatcg gcgaagtcgg
     2041 ggccgcgcat cggatggatc cagatgggca gatcgtgttc ggccatgcgg cggaagatcg
     2101 ggcggaattc cggtcggctc agcggttgat tcgcgacgtg ggtgaaaatc tgcaccccgc
     2161 gcgcgcccaa agcgccgata gcacggtcgg cttccgcaat ggccgcatcc atattgttca
     2221 tcggtagtgc ggcgacaaaa gcagggaaat agtcggtgtg cctcgcacag atttcagcga
     2281 gcgcatcgtt cgcaatccgg gcgagttcgg gtgtgaggtc aggtccggca atcagttcga
     2341 gcggcggatt ggcgagcgac agaacgttct gcagatcgcc gaattcatcg agcaatcgaa
     2401 gcctcgcttc gacgtcgcat agcgtcgcga ggcgcggaaa cgcagtgacc gccgcgtggc
     2461 ccggtatcag cgctgcaaga cgctccaggt acggcttcgg catgaagtga ttgaagatat
     2521 caattttcat gtgtcatgac ttcaaacggg cttcgcctgc gtgatctctt cgagcacgcg
     2581 acccttggtc tcgacgccca ctgcgatcag cgcgaaggag gcgatcagat aagagagagc
     2641 gaaggtatag agcgtccagc cgccgagccc gtaagccagc atgaagccaa ccaccgaggg
     2701 cgcgaggaaa gagccgatgc gcccgatacc gaagaaccag ccgactgccg tggcccgcag
     2761 atgggtcggg tgcagttcgc cgacgtagac gctggtggcg ccgtatacgc cggggttgac
     2821 ccacccgacc aggaaggccg tgacataggt ggcaaagcct gtcgacatcg caaacgccag
     2881 atggaagaag gcgcccacga aataatacag gaaaagaacc gggcggcggc catagcgatc
     2941 gatcagaaag ctgcaactcg aatagccgaa gaagcctccg atcgcctgga ccagttggaa
     3001 agcgatggca tccgtcagcg gtatcccgcg ctgcgtcagg attgtcggca gcatgaacag
     3061 aatgccgttc gaggcccaca agaatccgaa tgagacgatc cagacaagaa tagtcgtgcg
     3121 cgcgcgcccc ggcgcgaaca gttggaatac ggtaaccccg gtcgggacgg caacctctgg
     3181 cttcactttc ggcgcggccc gtatttcctc cgcggtcaac gaacggccaa gcgctcgccg
     3241 ctcgatctgc gcaacggtcg cttctgcctc cgcgaggcga cctttgctga gcagatagcg
     3301 aaccgattcc ggcatgtaac ggcgaacgaa aaacaagagc acggcgggaa cgacgccgac
     3361 cacgaacagc gcgcgccagc caagcgtcgg gatgatcaac aggctcagga gcgccgcgac
     3421 gatcagaccc accggaaacg ccgccatgat attgcccgtc atggcgccgc gaatgcggcc
     3481 cgggctgaat tcggaggcaa gcgtggacgt caccgggact tcgccgccca gaccgaagtt
     3541 cgagaggaac cgcgacacag ccagcgaggt gacattccat gacgcggcta cgagcccggt
     3601 gaacagcgca aatatgccga ccgtcgccgc aaaagaggtg cgccggccga tgtaatcgga
     3661 caccgttccc cagaataacg cgccgaaaaa tgccccgatc agtccgaccg tcgccagcag
     3721 gcccgattgg gctggaccaa gcccaaactc gcgggcaatg ggcggcaggg cagccccgaa
     3781 catggcgaaa tccgccgcgt cgaacatgat gccgaaaaac gccaggatga aaaaacttcg
     3841 gtgccaacgc gaaaacggca gctgctccag ccgtgcaatg agttccgccg attttgcttc
     3901 gtccgcgaca gccatgcgct tctggggtta aagatcgcct tcctttacga gagactagca
     3961 gaaaagtctt gggtttcgct tcgatcaccc caggctacaa ggacatcgcc atgacaagct
     4021 cgcggctgct tcgctggctc agccggtcgc cggtgcaaac attcattctt tgtccgctgc
     4081 ttgtggtcgc attcgagctg gtccggcagc gcggagagct gaccaccgag cctgcgggcg
     4141 ttgttctgct cgcctggggc tatctgcaat atctgctggt cggacgcttt cgcactcgtg
     4201 tgggcggcgg cggtcccgga tttggcgtgc cgccggatcg gatcgtggag gacgggcctt
     4261 atcgttacgt ccgcaatccg atgtacctcg ggcacctgat tgtcatgctg ggcttggcgg
     4321 tgacgttcgt ctcgtggttt gcgctcatcc tgttcgccgc gcgcgcgctc tggtttcacc
     4381 gccgcgtgct cgaggacgaa gcgcgccttg agaagatgtt tggaccggct tacctcgatt
     4441 atcagcggcg ggtgaagcgc tggatcccag gtctcttgtg aagggacgca gcgctttagg
     4501 ctcaggtccg cttcgcctgg acgggaacgg ccccattgtg cgttgcttgt gccgcgcgct
     4561 cagcgctgcg gcgccagcgg cgcatgaccc gagagcttgg ccaaggcggc ctgacgcacc
     4621 gtctcgcgac gcaggacatc cgcgagcttg tccggcgtgt tgtcggccgg cacggtcatg
     4681 ccgagcgcct ccatgcgctc gcggaacggc cgcgactgca cgaccgcgtt gagctctttg
     4741 ttcagaaacg cgatgatcgc gggcgccgtc ttggcgggcg ccgccagtcc gacaaacccg
     4801 ctatagtcga aattcggaaa tcccagctcc tgcatggtcg gcacatcggc ccagctcgca
     4861 atccgctgcg gagcggccac cgccagcgcg cgcactttgc catcgtcggc gagcggcttg
     4921 gcctgggcgt agaacgcgaa cgcgccctcg atgagaccag ccgcgacgtt gcgggcggcc
     4981 tcgccggagc cgcgatacgg gacgcccacg atctcggttt ttccgagcga attgagctgc
     5041 gtcagcgcga tcgccggcga ggtcgcggga ccggaggtgc cgaagctgat tttcttcggg
     5101 ttggccctgg cgtcggcgag gagctccgcg agtgatttgt aggaaagctt cgcatcgatg
     5161 atgagaacca gcggcgggcc ctcgacgatc agtccgatca tggcgaagtc gtcgaccgcg
     5221 ttatacggca ccgggatata gtgcaggttc tgcgcatcgg cgatcgagtt ggcgaacaat
     5281 gtgtagccgt cgggcgcggc acgcacggca taggtcccac ccacgacgcc ggagccgccg
     5341 ggtttgttct cgatcacgat gttctgcccg atccgctcct gcatcatcgc cgacagaacg
     5401 cggaccggaa cgtcggtcaa gccgccgggc gcgaacggca ccacaatggt aatggctcgc
     5461 gtcggataat cctgcgcctg tgcggccaca ggcgcggccg cgatgagtgc ggccactatg
     5521 ccgaccccta gagccctcgt catgattggc ttcccgttca tgtcatccaa cgcgccagca
     5581 taacaatccg aactggtttg tgcatcgatt actcgatccg gatgccggcg ctggccttcc
     5641 ccatatcttc ccctcccgcg cgtacaatgc caagcttgtg cggcctcctc acgacccatt
     5701 ggaccagcca cgatgagcaa taagagcgcg ttgcgcgcgg cggagccgct gcttcctgtc
     5761 gagctcggcg ccggcaaggt gaagtttgcg caaggcatga aggccggccg ttgggttttc
     5821 gccacaggat tgatggcgca ggatttccgc aacggcattg cgcccgatgt gttggccact
     5881 cgcgcgccgc atgccgggct tcccaagcgc gagaaggagg cgcagcgcat cttcgaaaat
     5941 ctcgagaccg tgctgtccgc cgccggcacc gagcgcagca atctggttcg caccgatcag
     6001 tactatacga cggtcacggc ggtgccgccc tatcagcagg tccggcgcga atttctgcgc
     6061 gggcgcgtcc cgccgtcgac ctcgatcacc cagaagcggc tgctgcttcc cgacgccgac
     6121 atgagcgtgc aagcgctggc ggtgatcccg gataaagact tcacggtgga gcacctcaaa
     6181 cacgagcacc tgaaaggacg ccccacctcc ggctattcgc ctgcgctcac ggtcggcgac
     6241 ttcatcttca ttcccggcat caccagcatg gcggtcggcg acgagccgcg ccgcaacggc
     6301 attgctgcgg cggcattgat ggcggaaggc gcgcaatggg gcgggcagcc aatcaagctc
     6361 gagaccgaat tcatcatcac gcagcgcatc gtgccgtcgc tgaagcttgt cggcgccggc
     6421 ctcgaggatg tggcgcatgc gcagatttat ctcacagacg ccgaggactt ttccgccttc
     6481 accgaggtct ggtcgcgaca tttcggggaa aacggaccga ccttctccgt catcccctgc
     6541 atcgagcatg ggcttgcgcc ctaccacggc aagatcgaga tcaacgtgct ggcggtaaga
     6601 ccgggcagtt ccgcgcgcaa gcgccacatc gatgctggcg tcacgacggc tttgcatcgg
     6661 cagccgcagg cgctgcgggc aggagaccta ctcttcatat ccggccttat ggccgcggat
     6721 cgcgatggcc ttgcgccatc ggcggcgcgc gatccgcgtc agccgtggtt ctcctcgccc
     6781 gcggaaatgc aggcggacat catcatcggc aacatcaagc ggctctgtga agccgccggc
     6841 acgtcgcttg agaacgtggt gcgcctccag caattccata ccgacatcag cgaattttat
     6901 gcagtttaca aggtttggga gcaccatttg gcaggccgtc cgctgccgtt ctcggcggtc
     6961 gaggtgccgg cgccgttgcc cgttcccggt gcgagcgtgc tgatcgaggc ctgggtctat
     7021 gcgccctgac gtctgtccgt ccaggtgcaa aggacgagaa gcctagcctg acctgctcct
     7081 ccaagccgcc acgggtaatg gtcacgtggg attaaaatgt gcgctgcggg tctgcgatga
     7141 tccgttcgtc cccgcgaacg cagggaccca gggctacagg ttgcggcccc tggattcccg
     7201 cttgcgcggg aatgaacgga caaggtcttg caacgcacat ttctacaaag cgacgatcaa
     7261 tgcccatgaa agtgaggcaa tcatcactcc acttaatgtc cacgccaaga tcaagcttgc
     7321 gaaacgattt aggccgtcca attttccaac gaagaccctc gatttcaagt accgcgaaaa
     7381 tccgtaaatg aaccaaatag cgacggccgg gctcagcgcg acgctacgta acatgttttc
     7441 gaaaacgcgc tgctgctctt tcgtgtcgag atcgttcaat gccgccacac tgacaagggc
     7501 gccgaacggc acgagtggca atcccgcgag tacgcctcgt actccatccg gcacgcgtcg
     7561 atgcgccgtc gcgaccaaaa agagcgccag cgcgagcgcg aacaggacga ttttcacctt
     7621 gctgctcagg atcgtgtgat cgatcgactg ccaagagaaa atgctcctat cagccgcggt
     7681 aaaatggact tcgggcaaga ggaaatggac gagcgcaacc aaaagcaaac cagcgagcgt
     7741 gcaaatgatc gcgtagggaa gttggcgcga ctccggaatg cccgctatca cttgcggtcg
     7801 gaaggcaatc aaaatccatg caccaacaac ggcaacggca agagaacccg cgagaactgt
     7861 ttcgtttaca tcgcccagca ggcgcaaaaa aacgaccatg aacacagcgc agagcgaccc
     7921 gtttatgacg ggaagccaca acatgctc
//
LOCUS       DATNOI010000156        21263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1704560,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000156 DATNOI010000000
VERSION     DATNOI010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 21263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21263
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704560"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(524..1594)
                     /locus_tag="VN968_05950"
     CDS             complement(524..1594)
                     /locus_tag="VN968_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXL28815.1"
                     /translation="MSVDAINATAATGIDPSRTRIAGAIKQAASTTGASFEYLLATAK
                     MESNFDPKASASTSSARGLFQFIDQTWLGTVKEAGSQLGYGQYADAISKSSSGSYSVS
                     DPAARTAILKLRDDPAAASSMAAVLTQSNSFKLTGQIGRRPTDSELYMAHFMGVGGAA
                     KLISNAEDHPNASGAALFPNAAEANHSIFYDRTGRARSVSEVYSVLTQRYDSAANSPA
                     TRTAMASVGVTPANAASASAAPVTAPIDTAAYLSSFPDTRNTVAAASQAGLKTAALQR
                     NEPIFRSLFQAGERSEPISPVVKELWGSSSSLTSVAPATSVAPASSVATATATSALSQ
                     TPTVRAPQPLDLFSDRNGTFSG"
     gene            complement(1810..2232)
                     /gene="hisI"
                     /locus_tag="VN968_05955"
     CDS             complement(1810..2232)
                     /gene="hisI"
                     /locus_tag="VN968_05955"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HXL28816.1"
                     /translation="MSADSHDVEEGLEFQPKFDASGLVTCVATDAATGDVLMVAHMND
                     EALRKTIESGEAWYFSRSRNAVWRKGETSGHVQRVVEMRLDCDQDAIWIRVEQTGAAC
                     HTGRRSCFYRRIDAGEGGARLTFVDADKVFDPTKVYRK"
     gene            complement(2281..2991)
                     /gene="folE"
                     /locus_tag="VN968_05960"
     CDS             complement(2281..2991)
                     /gene="folE"
                     /locus_tag="VN968_05960"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HXL28817.1"
                     /translation="MDALIKPLRPSKPSDVKPADSKLPEIRPAELDPAEFLAAAVRAD
                     QPRPSRAEAEAAVATLLGYIGENPDREGLLDTPRRVVEAYDELYQGYHQCPAEVLNRT
                     FGETAGYDDFVLIRDIEFTSQCEHHMMPFYGRAHIAYTPVERVVGLSKLARLTDIFAR
                     RLQTQEHLTAQIAAAIDEVLKPRGVAVMIEAEHTCMSVRGVAKHGASTFTSRFTGMFR
                     DNPAEQARFMSMVRGPQR"
     gene            3253..3708
                     /locus_tag="VN968_05965"
     CDS             3253..3708
                     /locus_tag="VN968_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HXL28818.1"
                     /translation="MLNDIYNKRIIELAGNIPRLGRLDAPDASATALSKLCGSTVKID
                     LRMDGAVVTDFAHDVKACALGQASSSIMARHVVGSTAGELRELRETVRKMLKENGKPP
                     QGKWADIALLEPVRDYKARHASTMLTFDAVVDAIGQIEAKAKQPVAAQG"
     gene            complement(3718..4647)
                     /locus_tag="VN968_05970"
     CDS             complement(3718..4647)
                     /locus_tag="VN968_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28819.1"
                     /translation="MRFVKSLDFRGWALVGAAALCIVWLGATATLGASAHAGVTLEER
                     KMPMRFSWIACQLNCRGSGWVSAVGIVTADSPQDFEEFARGRQLNGATIVLDSSGGSV
                     NDSIALGRRWRSLGALTAVGVSTATQAPQGARASVAPEAYCESMCVFLLLSGKVRYVP
                     EAAHVRVHQIWMGDRADDAKAASYSAQDMMIVERDIGRLAKYTFDMGGAGDLLSMALS
                     VPPWEELHELSREELRQTNLVTTEAVAEVLPELDGPNTPVAELTAKPVQDRFASELVR
                     AEKPQPAPVSAKSTKTAEANVPGGAVAVAAPAQ"
     gene            4889..5218
                     /gene="yidD"
                     /locus_tag="VN968_05975"
     CDS             4889..5218
                     /gene="yidD"
                     /locus_tag="VN968_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXL28820.1"
                     /translation="MKYSHCADCAGGVLRIPRNAGRAMIWLYRHTLSPLVGYNCRHLP
                     TCSVYGDEAIERFGLWAGGWMTLARLLRCQPWGTSGIDNVPLARPPGARWYLPWRFGR
                     WRGVNAP"
     gene            5427..5591
                     /locus_tag="VN968_05980"
     CDS             5427..5591
                     /locus_tag="VN968_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28821.1"
                     /translation="MRWKISHHDDPRQIDFLTILALLIMIVAAYWYFTHHPDPPSTTA
                     FIEPSQSVRW"
     gene            complement(5670..6893)
                     /locus_tag="VN968_05985"
     CDS             complement(5670..6893)
                     /locus_tag="VN968_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28822.1"
                     /translation="MKIAAVAAAMFLAAAARAHAADTATADDTARFLAGMPPSAASPL
                     TPLIQDPSWQQHAKFFDAAFGQLEQRQLSKIRNWADANLSAPRPTMFYMFSGPDFLYA
                     DAFYSKATTYVLSALEPVGAVPDLTRLPRGGIGSNLYDVERSLGSILSFSFFITKEMK
                     TDLRAGQISGTLPLLYVFLARSGKTIRDVSLVALDDKGTAYFANENAGSNPTRGVRIV
                     FASSDGQEKTLYYFSTDLSNRGVKSSGFLQFCQTLAPAGSLIKSASYLLHSGNFTTVR
                     DFLLANSATIIQDDSGIPLANYDPRKWRFFPFGRYAGPIDKFPSAYQPKYAELFRRAQ
                     PMDFGIGYRWRSFESNLLLSVQLPSDSSGSADATPSAPAAEPLQPLQPKPPRPRKPRP
                     PTFLDPPRDFFWFWR"
     gene            complement(6908..8281)
                     /locus_tag="VN968_05990"
     CDS             complement(6908..8281)
                     /locus_tag="VN968_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXL28823.1"
                     /translation="MAASETGSAAWPGRLSGGRTTVSVIVATAIVVADMVGVGVFTSL
                     GFQVKDIPSGFSILLLWTVGGIVALCGVFSYSELGAMFPRSSGEYNFLSRAFHPAFGF
                     LAGFVSATVGFAAPVALAAMAFGEYGKAVLPGAPPLALAIGVVWLVSIVQLGGVRHSS
                     TFQLISTILKVVLIIAFLTAGFVIGTPQPVSFAPQASDLAHVASAPFAIGLVFVMYSF
                     SGWNAATYIIGEMHTPQQNLPRALLAGTLIVLVLYVALNAVFLYTTPIDRLSGQLDVA
                     RIAGSSIFGEIGGRIVGAMICIGLVSSISAMMWIGPRVMMTMGEDIPALRVFARKSSN
                     GAPAFAILFQLAVANLMLFTRSFEAVLDFIQFALLFCSFFTVLGVIKLRTTQPDLPRP
                     YRAWGYPVTPVVFLLVTAFMMYYLLTERPVQSLLGALMMISGLLIYAIFRKRADPGPA
                     TLSASRE"
     gene            8651..9898
                     /locus_tag="VN968_05995"
     CDS             8651..9898
                     /locus_tag="VN968_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL28824.1"
                     /translation="MSHARAQAAQHYRARPRLRRIALLAALVPLSLALVQCGKAPNAG
                     MLAANSQDSAASFEDRFPATSFNDRFTGTNEDFVPRQSSDTPRSRSAQTGLAPYRVAS
                     LSPTVPFQLPPIREEQTTLVSLKSSAFPYLGNNPRTDEPFLNVSKGDRRGHRGLGGKV
                     FWQDETYNDNRALMHVPENFDIRKPGVIVVFFHGNGATLERDVRDRQLVPQQISESGV
                     NAVLLAPQLAVDAADSSAGKFWQPGGLKRFVAESAEHLAGLYGDRGTARAFANMPVVI
                     VGYSGGFMPAAWSLEVGGLGNRVRGVFLLDAVYGELDKFASWIVNNRSGFFVSSYTHY
                     TKRHDDELIKILKEKGIAISEDMDGPLRPGSVVFVETQEGVTHRDYVTHAWTENPVKE
                     LLVKMAATPALTRMAASAPSPAK"
     gene            10027..10677
                     /locus_tag="VN968_06000"
     CDS             10027..10677
                     /locus_tag="VN968_06000"
                     /inference="COORDINATES: protein motif:HMM:NF040431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HXL28825.1"
                     /translation="MRDFRNAKAMAQTLRAALATKGVKITVGQSLELIAEIFGLADWN
                     TLAAAIRAEKSISREKVSPPLPAVGSSSFPPPSAALAVTLHRAFGFASQRKHEYATLE
                     HLLLALIDDTDASAVMRVCSADLGALAHKLVGYLDNELKRLVVDNGANSKPTAGFQRV
                     VQRAELRGKELGRSMVTGANILEAITSETQSPAARLLGEQGMTRQHLLNVITHGTA"
     gene            complement(11104..11286)
                     /locus_tag="VN968_06005"
     CDS             complement(11104..11286)
                     /locus_tag="VN968_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28826.1"
                     /translation="MPTGLFKTSDQSVQVDYDGVSIPIPRSTYENNGYKPDFDNLPLE
                     AEYMAAKEKLGNVPKP"
     gene            complement(11557..14013)
                     /locus_tag="VN968_06010"
     CDS             complement(11557..14013)
                     /locus_tag="VN968_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXL28827.1"
                     /translation="MSAEIPSVRLVRGYCGLCIARCGTVATVTDGRFTRLDADPTHPT
                     GAAICAKGRAAPELVYHKDRLTRPLRRTRPKGDADPGWEEISWDAALDQTAAAMRRIA
                     EQHGPEAVAFSQSSPSTTAIADSAGFVRRLMNAFGTPNLVSHLELCGWGRGFATRYVF
                     GVGSVATGSAGGAMADIAESGCLILWGYNPSFSRITHATATVAGLKRGMKLIVIDPRN
                     VGLANKADVWLRVRPGTDGALALGLANIMIERDWYDQDFIRAWSNGPLLVRSDTDRLL
                     KAEDLASGGSPGQFVAWDSLAKGIVSYDPVAGRYAAPGEHLALRGEYIVATRDGAIGC
                     QPVFERYAALCKRYSSEVIEATCWIPREQLQEAAGIIWHSRPVSYYAYSGHEHHANVT
                     ETARAMAMLYALTGSFDAAGGNVLLPAVPAGSITGEDLPAAKRLAPAIGFAERPLGPA
                     RWNHASTQDFYRAVLEGIPYPVRGLIGFGTNLLLAQGDPVRGRAALAALDFYAHADLF
                     MTPTAALADIVLPVASCFEREALKTGFEISEAAQSHVQLRQAIVAPPGQARSDTDFIF
                     GLAAHLGLGEQFWNGDIDAAYREQLGPSGITLDQLRAEPAGLRKPLKTRHTKHAAGDD
                     EGNPCGFPTPSRKVEFWSETFLDHGYPALPDFVEPPTGPVARADLAASYPLILTCAKP
                     SLFCQSQHRALPGLRKRALHPEIELHPATAAARGIKNGDWVTVRTPAGGMRARARFSP
                     TLDPRVVVGEHGWWQGCDELGAADSDPFNPNGTNFNLTVDTAVRDPVGGTPSHRSNLC
                     EVQLVRGSQAKQPFRSASLT"
     gene            14303..15748
                     /locus_tag="VN968_06015"
     CDS             14303..15748
                     /locus_tag="VN968_06015"
                     /inference="COORDINATES: protein motif:HMM:NF028438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HXL28828.1"
                     /translation="MAIQAPSRIRVRFAVAALWAVCVFYAVAHAASPVPDGVSLSEST
                     VTWSTVNYATDAENGFVSGSLDKKTIVDRTFRTHVLENRYLKVTLLPEFGGRILSIIY
                     KPTGHEQLYRTEVGVPYGIEGGNFYYDWLMVYGGIFPTFPDAEHGKTWLKPWDFKVVK
                     ESAGEVTVSMSLKDDFAYAAAPRKFRSGSTGIEATYYVTLKADRAALDARVVLKNPRD
                     KTIDYEYWTCTTLAPGSDPKNPKTTGGAEIIAPIQAYSTPHWSASLADGDKSLGLGQS
                     RFENLRYFRNWPTMGIAYAAPDMQGGNFWGVINHDNEEGIIRIADNTVTPGLKMWTWG
                     FPSFTNETDARKDPNEARPYVELWAGVSDQFFHSARFPALGEVSIPETYSPTVGMSSV
                     THANENILINFSADGASVNLQFFSIEPATPLRLMLKRGDAVLFNDAVEADPKTGNRIS
                     ATIPASSGEQVQLTIRTAEGKELIAAETKIK"
     gene            16663..17592
                     /locus_tag="VN968_06020"
     CDS             16663..17592
                     /locus_tag="VN968_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HXL28829.1"
                     /translation="MPGSLDDQLGFAPDYDSPVPYMQRTRDYYTAIGYTTPYRWAHYV
                     DAPFQPLKKPLAQSRVTIITTAAPYDPAKGDQGPGAAYNGGAKFYKVYDGDTSKQHDL
                     RISHIGYDRKHTTATDSGTWFPLGLLLKAASSGRIGEVAPRFFGAPTNRSHRVTIEVD
                     APEILARCLADKVDVAVLVPNCPVCHQTSSLVARHLEAGGISTVVMGCAKDIVEHAAV
                     PRFLFNDFPLGNSAGKPHDIESQALTLELALGLLEHASGARTTMQSPLRWSEDASWKL
                     DYNNISQMSPEELARRRSEFDRQKEIARGNRAA"
     gene            17658..18851
                     /locus_tag="VN968_06025"
     CDS             17658..18851
                     /locus_tag="VN968_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXL28830.1"
                     /translation="MTRPFEGVKILDFTQVLAGPYASYQLALLGADVIKVERREGEDM
                     RRTPLSREWADRGLAPGWQAINGNKRSLTLDLQKPEAITIVTQLAASADVVMENFRPG
                     VMDKLGIGYAALSAVNPRLIYCAISGFGQTGPERSGAGYDGKIQAMSGIMAITGHETT
                     GPTRAGFAVCDVLSGATAAFGVSSALFQRTHTGKGQMVDVSMLEATLAFLSGQVADYA
                     VAGHRQQLSGNQAVSRRPTANLFKAGDGYLLLAVNSEKQYRALMAALGRADTLEDPRF
                     SDWFARQLNEPALRAIIEEALSKKGSREWEKTLEAAGAPCASIWKVEEVIDHPQISAR
                     QAIQEIDTPYGRLRFAGSGFQLAHGGGRLDSMAPELSAHTDEVLTSLGYDADAIADLR
                     ARNIV"
     gene            complement(19032..19775)
                     /locus_tag="VN968_06030"
     CDS             complement(19032..19775)
                     /locus_tag="VN968_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXL28831.1"
                     /translation="MQKVQVENNADGTTLITINRPERRNAICAETALMLQKAFQDFER
                     SGTQKAAVLTGAGNEAFSAGADTGNLPELWRCIPTVGFETNKPIISAVGGWCVGGALV
                     LSMMCDLTVAAENARFSYPEARLGFTGGLIAGLAARIPHKVAMEMMLLSRILDPQRAY
                     NIGLVNEVVPTGQQVQVALKMAREMAEFSPLVLKTLKRFVTEGVLAQGPSEQSGRAQR
                     ALHEVKESEDADEARSAVKEKRKAVYKGR"
     gene            20043..>21263
                     /locus_tag="VN968_06035"
     CDS             20043..>21263
                     /locus_tag="VN968_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL28832.1"
                     /translation="MGSEHVERRLAAILAADVAGSCRLIGIDEEGALARLKALRKTLF
                     DPKITDHRGRIVKNTGDGALVEFGSVVDAVRCAVEIQRGMAKHNIDVPQVKRIEFRIG
                     IHVGDIIIEDHDIFGDGVNIAVRLEGIAEPGGVSISDDAHRQIRGKVDITFENLGSQS
                     LKNIAEPMRVWRVPNGLVAPPVPNRPPVDDVGPLALPDRPSIAVLPFQNMSGDPEQEY
                     FADGIVDDITTALSRFKLVFVIARNSSFAYKGKAVDIKQIGRELGVRYVLEGSVRKAA
                     GKVRITGQLIDATTGVHLWADRFEGDLSDIFALQDQMTEGVVSAIAPRMFQTEIDLAV
                     RRSNNLSAYDLCLRASSHSKSWTRGGLAEALRLASRALEIDPRCGYAATLAGDCHLGN
                     VRQGWAADPKSEIAE"
BASE COUNT         4213 a         6504 c         6585 g         3961 t
ORIGIN      
        1 tcgttctcgg acaaatccga gatcaaccag gcccgcgcca aagcccgcgt cgcctctgcc
       61 cgctcgcctg ccggagcggt acgtatccac gtgatgaact gccgaacgat cattttcctg
      121 ccggcttacg caacctgaaa acgacaccga gatgccactg tcatacaaaa taagccacga
      181 cgcttaacaa agcgttcacc ataagcggct ggttttgttg acgttttgtt aagggcgcga
      241 aggtggcggg gtaagcgcag acttcgccgc gtgcgtgcct cagcctctcg cgagtcattc
      301 cgggatggtc cgaaggacca gacctcagat gcgcaattgc gcatcgggga atctcgagat
      361 tccgggttcg atgcttcgca tcgccccgga atgacggctg taggtttgtt cgcggagcct
      421 acctcggccg cgccacggat tgcttcgcgg agcctgtcat cgggcgcgca ttcgcgcgac
      481 ccgttggctc gcaatgacgg ctggttccgc atgctcgcta cagctacccg ctgaaggtgc
      541 cgttgcggtc gctgaagaga tcgagcggtt gcggcgcgcg gacggtcggg gtttgcgaca
      601 gcgccgatgt cgctgtcgcg gtcgcaaccg agcttgccgg cgcaaccgat gttgctggcg
      661 cgacggaggt cagcgacgag gagctgcccc acaattcctt caccaccggc gagatcggct
      721 cggatcgttc gccggcctga aacagcgagc ggaagatcgg ctcgttccgc tgcagcgcgg
      781 cggtcttgag gccggcttgc gaagccgctg ccacggtgtt gcgggtatcc ggaaagctcg
      841 agagataggc ggcggtgtcg atcggcgccg tcacaggggc cgcactggcg gacgccgcat
      901 tcgccggtgt cacgccgacc gacgccatcg cggtgcgcgt cgccggtgaa ttggccgcac
      961 tgtcgtagcg ctgtgtcaat accgaataga cttcggagac gctgcgcgcg cggccggtgc
     1021 ggtcgtagaa gatcgaatgg ttggcctccg ctgcgttcgg aaacagcgcg gcacccgagg
     1081 cgttcggatg gtcctcggcg ttcgagatca gttttgccgc gccgccgacg cccatgaaat
     1141 gcgccatata gagttcgctg tcggtcgggc ggcggccgat ctggccggtg agcttgaagc
     1201 tgttggattg ggtcagcacc gccgccatcg acgaggcggc tgcgggatcg tcgcgcagtt
     1261 tcaggatcgc ggttcgcgcg gcgggatcgc tgaccgagta gctgccggag gatgatttgc
     1321 tgatggcgtc ggcgtattgg ccgtagccga gctgggagcc ggcttccttg acggtgccga
     1381 gccaggtctg gtcgatgaac tgaaacagcc cgcgcgccga cgaggtcgaa gcgctggcct
     1441 tcggatcgaa attggattcc attttcgcgg tggcgagcag atattcgaag ctggcgccgg
     1501 tggtgctggc ggcctgtttg atcgcgccgg caatccgcgt gcgcgacgga tcgatgcctg
     1561 tcgcggcggt ggcgtttatc gcgtcgaccg acatctaaag gtgccccgct ccccgcgaaa
     1621 cgacttacag ggcgcccaat cgcgctcatg cccggcgtca cactgggatg attgtggtta
     1681 acggagtatt aagaaagttc cagccgtcat tccgggacac gcgtcttcgc gtgggcccgg
     1741 aatccataac ccctgcaggg agtatggatt ccgggcctgc gccaagtggc gcatcccgga
     1801 atgacggagt tatttccgat aaaccttggt gggatcgaac accttgtccg catccacgaa
     1861 cgtcaagcgc gccccgccct cacccgcgtc aatcctgcga taaaagcacg agcgccggcc
     1921 ggtgtggcac gcggcccccg tttgctcgac ccggatccag atcgcatcct gatcgcagtc
     1981 cagccgcatc tcgaccacgc gctggacatg gcccgaggtt tcgcccttgc gccacaccgc
     2041 gttgcgcgag cggctgaaat accacgcctc gccgctttcg atggtctttc gcagggcctc
     2101 gtcgttcatg tgcgcgacca tcagcacgtc gccggtcgcg gcgtcggtcg cgacgcaggt
     2161 cacaaggccg gaggcgtcga atttgggctg gaactcaagc ccttcctcga cgtcatggct
     2221 atcggcggaa atatctgcgg atgtggacac gggcagccct cgcgaaatct gaaccgaggg
     2281 ttagcgctgc ggccctcgca ccatggacat gaaacgcgcc tgctccgccg gattgtcgcg
     2341 gaacatgccg gtaaagcggc tggtgaacgt cgaggcgccg tgcttggcga cgccgcgcac
     2401 cgacatgcag gtatgctccg cctcgatcat cacggcaacg ccgcggggtt tcagcacctc
     2461 gtcgatcgcc gccgcgatct gggcggtcag atgctcctgg gtctgcaggc gccgggcaaa
     2521 gatatcggtg agccgcgcca gcttcgacag ccccaccacc cgttccaccg gcgtataggc
     2581 gatgtgggcc cggccgtaga acggcatcat gtggtgctcg cattgcgagg tgaattcgat
     2641 gtcgcggatc agcacgaaat cgtcatagcc ggcggtctcg ccgaaggtgc ggttcaacac
     2701 ctcggccgga cactgatgat agccctgata gagctcgtca taggcttcga ctacccggcg
     2761 cggggtatcc agcaggccct cgcggtcggg gttttcgccg atatagccca agagggtcgc
     2821 gaccgccgct tccgcctcgg cgcgcgaggg acgcggctgg tccgcgcgca cggcggcggc
     2881 gaggaattcg gcaggatcaa gctcggccgg acggatctcc ggaagcttcg aatccgcagg
     2941 cttgacgtca gaaggcttgc ttgggcgtag cggtttgatc aaggcgtcca tgtcaactcc
     3001 gttcgaccgg cctcaaaagg ccggagtgtc accgggcaga cggggcggct cgccgccgga
     3061 cgcctgagtc ccaaaaatac caataccgca gcggccgaat aggcttctat tcgggccgcc
     3121 atgggaaacg gctggactgt atttccaccc tttcgaccca tatatagggc tactgacagc
     3181 ggtcgccaag agcggttgcc aagcgcgatt gccccccgca agcccgaggc ccccatctga
     3241 tgctgcaatc ccatgctgaa tgacatctac aacaagcgca tcatcgaact ggccggcaat
     3301 ataccccggc tgggccggct cgacgcgccc gacgccagcg ccacggcgct ctccaaatta
     3361 tgtggctcga cagtcaagat cgacctccgg atggacggag cggtggtcac cgactttgcg
     3421 catgatgtga aggcctgcgc gctcggccag gcctcgtcct cgatcatggc gcgccacgtc
     3481 gtcggctcga ccgcgggcga actgcgcgaa ttgcgcgaga ccgtccgcaa gatgctgaag
     3541 gaaaacggca agccgccgca gggcaagtgg gccgacatcg ccctgctcga gccggtgcgc
     3601 gactacaagg cgcggcatgc gtcgaccatg ctgacttttg acgccgtggt cgatgcgatc
     3661 ggccagatcg aggccaaggc gaagcagccg gtcgcggcac agggttagag ttcgccccta
     3721 ctgtgccggc gccgccacgg cgacggcgcc gccgggaacg ttggcttccg ccgttttggt
     3781 ggatttggcc gacactggcg cgggctgcgg cttctcggcc ctcaccaact cgctcgcgaa
     3841 acgatcctgt accggcttgg ccgtgagctc ggccaccggc gtgttcgggc cgtccagctc
     3901 gggcagcacc tcggcaacgg cctcggtggt aacgaggttg gtctgccgca attcttcccg
     3961 ggacagttca tgcagctctt cccaaggcgg cacgctcagc gccatcgaca gcaagtcgcc
     4021 cgcgccgccc atgtcgaagg tgtatttggc gagccgcccg atgtcgcgtt ccacgatcat
     4081 catgtcctgg gcgctgtagc ttgccgcctt ggcgtcgtcg gcgcggtcgc ccatccagat
     4141 ctgatgcacc ctaacatggg cggcctcggg cacgtagcgc accttgcccg acaacagcag
     4201 gaacacgcac atcgactcgc aataggcttc gggcgcgaca ctcgcccgcg cgccttgcgg
     4261 ggcctgggtc gcggtgctga cgccgacggc ggtcagcgcg ccgagactgc gccagcgccg
     4321 gccgagcgcg atcgagtcgt tgaccgaacc gccgctggaa tccagcacga tggtggcgcc
     4381 gtttagctgg cgtccgcgcg cgaattcttc gaaatcctgc gggctgtccg cggtcacgat
     4441 gccgaccgcg ctgacccagc ccgagcccct gcaattgagc tggcatgcga tccagctgaa
     4501 ccgcatcggc atcttgcgtt cttcgagggt gacgccggca tgcgcggaag cgccgagcgt
     4561 agcggtggcg ccaagccata caatgcataa ggcggcggcg ccgacgagcg cccaaccgcg
     4621 aaaatcgagt gacttcacaa acctcaattc ggttccttcc caagctccca agccgagagt
     4681 ggtagcggcc ccagttcagc gccagaatga ttctcgtcac acaggcgtat caaagggtaa
     4741 ctttacggca aagaagcgtc tattaacatt tgctgcaccg cccccaaata aatgcgatat
     4801 ttgggcaggt gttactcaat tgcatgcgtt gcacggttcc gtgcatcgga accgggcaaa
     4861 tacaggctca acaacaggca cggcgcctat gaaatattcg cattgtgcgg actgcgccgg
     4921 cggggttttg cgaattccgc gcaacgcggg acgcgcgatg atctggcttt accggcacac
     4981 gctgtcgccg ctggtcggtt acaactgccg tcacctgccg acctgttcgg tctatggcga
     5041 cgaggcgatc gagcgctttg ggctgtgggc cggcggatgg atgacgctgg cgcggctgtt
     5101 gcgctgccag ccctggggca cgtcggggat cgacaacgtg ccgctggcca gaccgccggg
     5161 cgcgcgatgg tatttgccgt ggcgtttcgg gcggtggcgc ggggtcaacg cgccctagag
     5221 tttgcttcag ccaggctgaa gcaaactcgt cgcttattta tttgtcgggg gcatgatctt
     5281 atccgaaaac cggtatccac ttttcgggat catgcccgag gaattctgct gcagcgcttt
     5341 cagcagattt cctcattgct gccatgatgg gaaccgcaac cccaccgccg cattggtttg
     5401 atgctccccc atggacggca agatcaatgc gctggaaaat cagccaccac gacgatcccc
     5461 gacagatcga ctttctcacg atactcgcgc ttctcattat gatcgtcgcg gcatactggt
     5521 atttcaccca tcaccccgat cccccgagca ccacggcctt catcgagccg agccaaagcg
     5581 tgcggtggtg aggaattttg tttgaggcgt tgacaggtat ccgctccgca tcaagtgcgg
     5641 ggcaggcttt cgcttgaacc cacggcagct tagcgccaaa accagaagaa atcccggggc
     5701 ggatcgagaa aagtcggcgg acgcggcttc ctgggacgcg gtggtttggg ctgcaggggc
     5761 tgcaggggtt cggccgctgg cgccgatggc gtcgcgtcag cgctcccgga actgtccgag
     5821 ggaagctgga ccgacagcag caggttcgat tcaaacgatc tccaccgata gccgataccg
     5881 aaatccatcg gctgagcccg cctgaacagc tcggcatatt tcggctggta tgcgctgggg
     5941 aatttgtcga tcggaccggc gtagcggccg aacggaaaga accgccattt cctcggatcg
     6001 taattggcaa gcggaatgcc ggaatcgtcc tggataatcg tggcgctgtt ggcgagcaga
     6061 aaatcgcgca cggtggtgaa attgccggaa tgcaacagat aggacgcgct cttgatcagg
     6121 ctgcctgccg gcgccagggt ttggcaaaac tgcaggaaac ctgagctctt gacgcccctg
     6181 ttggaaagat cggttgagaa ataatacagc gtcttttcct gaccatcgct gctggcgaac
     6241 acaatccgca ccccccgcgt tgggttgctg ccggcatttt cattggcgaa atacgcggtc
     6301 cccttgtcgt cgagcgcgac cagactgacg tcgcggatgg tctttcccga gcgcgccagg
     6361 aagacgtaca acagcggcaa ggtgccgctg atctgtccgg cgcgcagatc ggttttcatc
     6421 tccttggtga tgaagaagct gaaactcagg atcgaaccca gcgagcgctc gacatcgtag
     6481 aggttggacc cgataccacc gcgcggcagc ctggtcagat ccggcactgc cccgaccggc
     6541 tcgagcgcgc tgagcacgta ggtcgtcgcc ttggaataga acgcgtccgc atagagaaag
     6601 tccgggccgc tgaacatgta gaacatggtc ggccgcggcg cggacagatt ggcgtccgcc
     6661 caattgcgga ttttcgaaag ctggcgctgc tcaagttgtc cgaacgcggc atcgaagaat
     6721 ttggcgtgct gctgccatga agggtcctgg atgagcggcg tcagcggcga agcggccgac
     6781 ggcggcattc ctgccaggaa acgcgcggta tcgtccgcgg tcgcggtgtc ggcggcgtgc
     6841 gcgcgcgccg cggcggcgag aaacatggcc gcagcgaccg ctgcaatttt catcggctgc
     6901 agcatcgcta ttcgcggctt gcggataggg tcgcggggcc cggatcggcc cgcttgcgga
     6961 agatggcgta gatcaagagg cccgagatca tcatcaaagc gcccagcaac gactgcaccg
     7021 gccgttccgt caagagataa tacatcataa aggcagtcac gagcaggaaa accaccgggg
     7081 ttaccgggta tccccaggcg cgatagggcc gcggcagatc aggctgtgtg gtgcgcagct
     7141 tgatgacgcc gagcacggtg aagaacgagc agaacaacag cgcgaactgg atgaaatcga
     7201 gcacggcctc gaaactgcgg gtgaacagca tcaggttagc taccgcaagc tggaacagga
     7261 tggcgaaggc cggcgcgccg ttgctcgatt tgcgggcaaa tacccgtagc gccggaatat
     7321 cctcccccat ggtcatcatg acccgcggac cgatccacat catcgcgctg atggaagaca
     7381 ccagcccgat acagatcatg gcgccgacga tgcggccgcc gatttcgccg aaaatgctgc
     7441 tgccggcgat ccgcgcgaca tcgagctgcc cggacagtct gtcgatcggc gtggtgtaga
     7501 ggaacaccgc attcagcgcg acatagagca cgagcacgat cagcgtcccc gccagcagcg
     7561 cgcgcggcag gttctgctgc ggcgtgtgca tctcgccgat gatgtaggtc gcggcgttcc
     7621 atccggagaa cgaatacatc acgaacacca gtccgatcgc gaacggggcg ctggcgacgt
     7681 gtgcaagatc ggaagcctgc ggcgcgaacg acaccggttg cggcgtgccg atgacgaatc
     7741 ccgcggtcag gaacgcgatg atcagcacga ctttcaagat ggtcgagatc aattgaaagg
     7801 tgctcgagtg cctgacgccg ccgagttgaa caatcgacac cagccagacg acaccgatcg
     7861 caagcgcaag cggcggcgcg cccggaagca cggccttgcc gtattcgccg aatgccatcg
     7921 ccgccagcgc caccggtgcg gcgaagccga ccgtcgccga cacaaagccc gccagaaacc
     7981 cgaacgccgg atgaaaggcg cgactgagga aattgtactc cccgctcgag cgcggaaaca
     8041 tggcgccgag ttcgctgtag gaaaaaaccc cgcacagcgc gacgatgccg ccgaccgtcc
     8101 atagcagcag gatcgaaaag cccgagggga tgtccttgac ctggaagccc aggctggtga
     8161 agacgccgac gccgaccata tcggccacga caatggcggt agcgacgatg accgagacgg
     8221 tggtcctgcc cccagagagc cggccgggcc aagccgcgct tcccgtttct gatgccgcca
     8281 tgtcgatcta cgccttacgc gtcacgcccg taattcttca tcgtgcaggc tcgccccgtt
     8341 caattcaagc aatgttaaca aggcttaaat acagctccgc accaggtatt attatacttt
     8401 caaagcgccc ccccggagtt ctacggcatt gcgaaaacaa ggtgatcggc ccacaatcgc
     8461 gacacgattt cgtgttcgct tgcgcgttcc gggggtgggg gagttttccc ggacgcttaa
     8521 cgttacctca acgttagcga gctgaaatac gccgatcgcc ggagggtgtt ggggcaaggg
     8581 gtggatggtc ggagccagtc cgaaattcca gacgtctgat cggttgggcc aggtgacacc
     8641 accgggtgcg atgtcacacg cccgcgcgca ggctgcgcag cactatcgcg cgcgtcctcg
     8701 actgcgccgg atcgcccttc tggcagcact tgtcccattg tcgctcgctc tcgttcaatg
     8761 cggcaaggcg cccaacgccg gaatgctggc cgccaactcg caggattcgg cagcttcgtt
     8821 cgaggaccgg ttcccggcga cgtcgttcaa cgaccgtttc acgggtacga atgaagactt
     8881 tgtgccgcgg caatcctccg acaccccccg aagccgcagc gcgcagacgg gcctcgctcc
     8941 ctacagggtg gcgtcgctta gcccgacggt accgttccaa cttccgccga tccgggaaga
     9001 acagaccacg ctggtcagcc tgaaatcctc ggcgtttccc tatctcggca ataatccgcg
     9061 caccgacgag ccgttcctga acgtttccaa gggcgaccgc cgcggccacc gcggcctggg
     9121 cggcaaggtt ttctggcagg acgagaccta caacgataac cgcgccctca tgcacgtccc
     9181 ggagaacttc gatatccgca aaccgggcgt gatcgtcgtg ttcttccacg gcaacggcgc
     9241 gacgctggag cgcgacgtgc gcgaccggca actggtgccg caacagattt cggagtccgg
     9301 cgtcaatgcc gtgctgctgg cgccgcaact ggccgtcgat gccgccgatt ccagcgccgg
     9361 caagttctgg caaccgggcg ggctcaagcg tttcgtggca gagtcggccg agcacctcgc
     9421 cggtctctac ggcgatcggg gcaccgccag ggctttcgcc aacatgccgg ttgtgatcgt
     9481 cggctacagc ggcggcttta tgccggcggc gtggagcctg gaagtgggcg gcctcggcaa
     9541 ccgcgtgcgc ggcgtgttcc tgctcgacgc ggtttatggc gagctcgaca agttcgcgtc
     9601 gtggatcgtc aacaaccggt ccggcttctt cgtcagttcc tatacgcatt acaccaagcg
     9661 gcacgatgat gaactgatca agatactgaa ggaaaagggc atcgccatat cagaggacat
     9721 ggacgggccg ctacggcccg gcagcgtggt atttgtcgag acacaagagg gcgtgacgca
     9781 tcgcgattac gtcacccacg cctggaccga aaatcccgtc aaggaattgc tggtcaagat
     9841 ggcggcaacg cccgccctga ccaggatggc tgcgagcgcc ccgtcgcccg cgaagtgacg
     9901 atccggcgcg ccttcacgcc gccgcatcgc cgcaacttcc ctgtcatatc cgccgcccga
     9961 taaaagatgc cgttgaggac gcgccggtca ggtgtccatc agttcggagg aggttcagat
    10021 gaagacatgc gcgatttccg caacgccaaa gccatggcgc aaacgctacg tgcggccctt
    10081 gctaccaagg gtgtcaagat caccgtcggt caaagccttg aactgatcgc tgagatattc
    10141 ggcctagccg attggaatac gctcgctgcc gcgatccgcg cggagaaatc gatttctcgc
    10201 gagaaagtct ccccaccact gcccgccgtg ggtagtagtt cgtttccgcc gccttcggcc
    10261 gcgcttgcgg tgacgctgca tcgggccttt ggcttcgcca gccagcgaaa gcatgagtac
    10321 gcgacactgg agcatcttct gctcgccctg atagacgaca ccgatgcttc ggcggtgatg
    10381 agggtctgca gcgccgatct tggcgcgtta gcgcacaagc tggttggcta cctcgacaac
    10441 gaactgaaga gactggtggt cgacaacggc gcaaactcca aaccgaccgc tgggtttcag
    10501 cgcgtcgtcc agcgcgctga gcttcgggga aaggagctgg gccgctccat ggtaactggc
    10561 gcaaacatcc ttgaagccat aacttccgaa acgcagagcc ccgctgcacg gcttctcggt
    10621 gaacagggaa tgacccgtca acacctattg aacgttatca ctcacggaac cgcatagact
    10681 gccgcacaag gaaggaccgc gcgaggcggg acgtcgcctc gcagcaattc ttgtcgctga
    10741 agtccgagtc tgtgcagacg tgccggcggg cactgagaat gtccgttgat cggggcagac
    10801 cggaagtggc tggcccgcgc tcaatgtgac gcaaaaaccc tcagccgaag tcaggtctcc
    10861 tgccttggtc cgcttcgcgc tattggcgcc tcaactgggc ctgcgcaatg gcttccaaaa
    10921 ctgtgttagg atttgcgagc aacgccggga ggatcaatcc ttgagtgagc cgctgacacg
    10981 tcgggcgact gtcatatgct ttttggagct tcacaacgtt ctttgaacag ccaaccccaa
    11041 ttccgtggag gcgtcttgct gaggatacag ccggtctaaa cgtcggccgg gcgcttctcg
    11101 cctttagggc ttggggacat tacccaactt ctcctttgcc gccatatatt cggcttcgag
    11161 gggcaggttg tcgaaatccg gcttgtagcc gttattctca taagtcgatc taggtatcgg
    11221 gatcgaaacg ccgtcgtaat caacctgaac cgactgatcc gacgttttga acaaccctgt
    11281 cggcatcgcg ccaggcccct tactttccgc taaagcatgc gacgggagaa ctacgcgagc
    11341 ggcttggtca accccatgcc agccacccaa acaaaaagac cgccgggtcg accgacagtc
    11401 ctttcgttaa catgcatgtc agttggggtt gacgcgctcg actttgcttt ggccgtatga
    11461 cactgccact ctggggctgg tctcttgttg gtgctagctg gtgcgagaac ctcgtcgatc
    11521 aaacccgact taaaaatgag caaggcccga aaaagatcaa gtgagcgatg ctgacctaaa
    11581 gggctgtttt gcttgagatc cccgcaccag ctgaacttca caaagattgg atcgatgcga
    11641 tggggtgccg ccgacgggat cgcgaacggc ggtgtcgact gtcaggttga agttcgtgcc
    11701 gttcggattg aacggatcgg aatcggcagc tcccaattca tcgcaaccct gccaccagcc
    11761 atgctcgccg acgacaacac gtggatcgag cgttgggctg aaacgcgccc gggctcgcat
    11821 tccgccggcg ggggtgcgca cggtaaccca atctccattc ttgatgccgc gtgctgcggc
    11881 agtcgcagga tgcagttcga tctccggatg caatgcgcgc ttgcgcaaac caggaagtgc
    11941 acgatgttgg ctctgacaaa acaggctggg cttggcgcac gtcaagatca aaggatagga
    12001 ggccgccagg tcagcgcgcg caaccgggcc ggttggcggt tcgacaaagt ccggcagggc
    12061 tggataaccg tggtcaagaa aggtctcgga ccagaattcc actttccgcg acggcgtagg
    12121 gaacccgcat ggatttccct cgtcgtcccc cgcggcatgc ttggtgtgac gcgtcttcag
    12181 gggtttccgc aatccggcag gttcggctcg aagttggtcc agggtgatac cactcggccc
    12241 gagttgctcg cgataggcag cgtcgatgtc gccgttccag aactgctcgc caaggccgag
    12301 atgcgctgcg aggccgaaaa tgaaatcggt gtccgaccgt gcctgaccgg gcggggcgac
    12361 gatcgcctgc cgaagctgaa catgcgattg tgcggcttcg ctgatctcaa atccggtttt
    12421 tagagcttcg cgttcgaagc aggaggccac aggcagcacg atatccgcca gcgcggccgt
    12481 gggggtcatg aacagatcgg catgggcgta gaaatcgagc gcggcgagcg cggcacgtcc
    12541 cctgacagga tcgccctgag cgagcagtag attggtgccg aacccgatga gtcctcgaac
    12601 cggataggga ataccttcca ggacagcacg gtagaaatcc tgagtggagg catggttcca
    12661 gcgcgcaggt ccaagcggac gttcggcaaa accgatcgcg ggtgcaagcc gcttcgccgc
    12721 aggcaggtct tctccggtga ttgacccggc tggcaccgca gggagcagca cattgccacc
    12781 ggccgcatcg aaactgccgg tcagtgcata cagcatcgcc atcgcgcgcg cggtttcggt
    12841 gacgttcgcg tgatgctcgt gcccgctgta ggcatagtag gaaaccggcc gggagtgcca
    12901 aatgatgccg gcagcttcct gcagttgctc gcggggaatc cagcatgtcg cttcaatcac
    12961 ttcggacgaa tatcttttgc atagcgctgc atagcgctcg aaaactggct ggcagccgat
    13021 ggcaccgtcg cgtgttgcga cgatgtattc gcctcgcagg gcaagatgct cgcctggggc
    13081 ggcgtagcgg ccggcaaccg gatcatagct gacgatgcct ttggccaggg aatcccaggc
    13141 cacaaattgt cccgggctgc cgccggaggc cagatcttcc gccttcagca acctgtcggt
    13201 atccgagcgc accaagagag gtccattgct ccacgcacgg atgaaatcct gatcgtacca
    13261 gtcgcgctcg atcatgatgt tagccagacc cagcgccagc gcgccgtctg ttccaggacg
    13321 cacgcgcagc cagacatcgg ccttgttggc aagcccgaca ttgcgcggat caatcacgat
    13381 cagcttcatt ccgcgtttca ggcccgcaac cgtcgccgtt gcgtgggtga tgcgcgagaa
    13441 cgaaggatta tagccccaca ggataaggca gccggattcg gcaatgtctg ccatcgcgcc
    13501 gccggcgctt cccgtggcaa cgctgccaac gccgaagacg tagcgcgtcg caaaacctcg
    13561 tccccagccg cacagttcga gatgcgatac caggttgggc gtgccgaaag cattcatcag
    13621 tcttcgaaca aagccggcgg aatccgcgat agccgttgtc gagggggacg attggctgaa
    13681 ggctacggct tcaggtccat gctgttccgc gatccggcgc atggcggcgg cggtctggtc
    13741 gagcgccgca tcccatgaaa tttcttccca tcccggatct gcgtcgccct tcggccgtgt
    13801 tcgccgcagc ggccgggtca atctgtcctt gtgatagacc agttcgggag ccgctcggcc
    13861 cttggcacaa atcgctgcgc cggttgggtg cgtcggatcg gcgtccaggc gtgtgaatcg
    13921 accgtccgtt acggtcgcga cggttccgca gcgggcgatg caaaggccgc agtaaccacg
    13981 caccagccgc acgctcggga tctctgctga catgcacaca ctccgtcgcc gtcatggtac
    14041 tgcgtatggc cggatcatgc caaccgtaac ggccgattgc cgccgctatc agattatctg
    14101 cgcaagcggt cctttgacag cgtcgtctcg cggatcagga acgcgaatac ctgcttttgc
    14161 atccgctttc agggggtttg accccagtcg gatattaccc tcattccggc gtattggctg
    14221 caggcgagcc gatcaagcct gcttcggccg gccgcgtcga tgtactagaa ttgtcgctcc
    14281 tgcgcattcg aaggcgacgc aaatggcaat tcaggctccc tcgcgtatcc gggtccgttt
    14341 cgcggttgca gcactttggg cggtctgtgt gttttatgcc gtcgcgcacg cggcgtcgcc
    14401 ggtgcccgac ggagtgtctc tcagcgagtc gacggtgacg tggagcaccg tgaactacgc
    14461 gaccgatgcg gagaacggat tcgtcagcgg gtcgctcgac aagaagacca tcgtcgatcg
    14521 cacgttcagg acccacgtgc tcgagaatcg ttatctgaag gtaacgctcc tgcccgaatt
    14581 tggcgggcgc attctttcca tcatttacaa gccgaccggc cacgaacaac tttatcgcac
    14641 ggaagtcggc gtgccttacg ggatcgaggg cggcaatttt tattacgact ggctgatggt
    14701 gtatggcggc atcttcccca cattcccgga tgccgagcac ggcaagacgt ggctgaagcc
    14761 gtgggatttc aaggtggtga aggagagtgc cggtgaagtg acggtgtcga tgtcgctgaa
    14821 agacgatttc gcatatgccg cggcgccgag gaagttcagg tcgggctcga cgggcatcga
    14881 agcgacttac tatgtcacgc tgaaggccga tcgcgccgcg ctcgatgcgc gcgtggtgct
    14941 gaaaaatccg cgcgacaaga cgatcgacta cgagtactgg acctgcacga cgctcgcgcc
    15001 gggatcggac ccgaaaaatc ccaaaacgac cggcggcgcc gagatcatcg cgccgatcca
    15061 ggcctacagc acgccccact ggtcggccag cctggccgac ggcgacaaga gcttgggcct
    15121 gggccagagc cgcttcgaaa acctgcgcta cttcaggaac tggccgacga tgggcatcgc
    15181 gtatgcggcg ccggatatgc agggcgggaa tttctggggc gtgatcaacc acgacaacga
    15241 agagggcatc atccgcatcg ccgacaatac ggtcacgccg ggcttgaaaa tgtggacgtg
    15301 gggatttccg tcgtttacga acgaaaccga cgcacgcaaa gacccgaacg aagcgcggcc
    15361 ttacgtcgaa ttatgggcgg gcgtgtcgga ccagtttttc cacagcgcca ggtttcccgc
    15421 actgggcgaa gtgtcgattc cggaaaccta cagcccgacc gtcggcatga gcagcgtgac
    15481 gcatgcgaac gagaacatcc tgatcaattt ctcggccgat ggtgcgagcg tgaatctgca
    15541 gttcttcagt atcgaaccgg ccacgccgtt gcgcttaatg ttgaaacgcg gcgacgccgt
    15601 attgttcaac gacgccgtgg aggccgaccc gaaaaccgga aatcgcattt ccgcgacgat
    15661 tcctgccagc agcggcgaac aggtgcagtt gacgatcagg accgcggaag gtaaggagct
    15721 gatcgcggcc gagacaaaaa taaaatagat ccttgccgac cgctcccgta tttgaacagg
    15781 gagcaaatag agccagcctt taaaggtccc agcgatagcg aatttgtttt caccggcgga
    15841 acatccgcgg ggttcatgcc caatgcactc gcgaacgtcg aagccccgga cagggcgtga
    15901 cgatatcagg gtagaaattt tacgccgtat atcttgccgc gacgccaaac gacttcgcat
    15961 ggctggcgct tcggacgatc cggcacaagc gccaattcga acctgtccct gacatcgatc
    16021 tcagcgtcgg tcataagctt ggctccgccc ggcgagacgt cgaatacaag gcattcgttc
    16081 ttcgtcattc cgtccaccgt aatccaggtc ggccgcttct gcagttgcct tcgcggttcg
    16141 cgctgtacct tcgcccttgg cttgtcaaca atcataaacg gccatccatc gttggaccta
    16201 attgcgatcg gccaggcgat cgtcaaaaac aggcgtcaga atgaagctga tttcgtaaag
    16261 ctcctcttag gacattgcct cgaaatttat cagtttaccc tgttcagcct gtgatgacgt
    16321 gaatggcgat gcggcgtccc aaatggcgga cctccggttc tggcacgttt tgagacatgc
    16381 caaggggacc ccgagggcat gcttcgcttg aaaacgcgct gaaagctgac gcgcatcggc
    16441 gactgaacaa gcaaccactt ggcgcgacga agtcttgcgc cgggcagtat tccgcccgca
    16501 atgtgatccg atcccatacg ccagcccgtc gcggacgaag gataaagcgg gcaccgaaca
    16561 tttgcggctg gatgtctgtt ctttgcggaa taagcggact atctttggtc gggccgaagg
    16621 ccgattgacg atctcttttg aaacgcagga aggacgcaaa ctatgccggg ctcgctcgat
    16681 gaccaacttg gctttgcgcc tgattacgat tccccggtcc cctatatgca gcggacccgc
    16741 gactactaca ccgcgatcgg atacacgacg ccgtatcgct gggcgcacta tgtcgatgcg
    16801 ccgttccagc cgctgaaaaa gccgctggcg caatcgcgcg tgacgatcat caccaccgcc
    16861 gccccttatg atccggcgaa gggcgatcag ggcccgggtg ccgcgtataa cggtggcgcc
    16921 aagttctaca aggtctatga cggcgacacg tcgaagcagc acgatttgcg catctcgcat
    16981 atcggctacg accggaagca caccaccgcg accgacagcg gcacctggtt tcccttaggc
    17041 ttgctcctga aggcagcaag ctcggggcgc atcggcgagg tcgcgccgcg cttcttcggc
    17101 gcgccgacca atcgcagcca ccgcgtaacc atcgaggtcg acgccccgga gatcctggcc
    17161 cgctgcctcg ccgacaaggt cgatgtcgcc gtgctggtac cgaactgccc ggtttgccac
    17221 cagacttcca gcctggttgc ccgccacctc gaagcaggcg gcatttcgac cgtggtgatg
    17281 gggtgcgcca aggacatcgt cgagcacgcc gctgtgccgc gcttcctgtt caacgacttc
    17341 ccgttgggta attccgccgg caagccgcat gatatcgaat cgcaggcgct gacacttgaa
    17401 ctggcgctgg gcctgctgga gcacgcctcc ggcgcgcgca ccacgatgca gtcgccgctg
    17461 cgctggagtg aggatgcctc ctggaagctc gactacaaca acatttcgca gatgagcccg
    17521 gaagaactgg cgcggcgcag aagcgaattc gacaggcaaa aagagatcgc gcgcggcaac
    17581 cgagccgcct gaccagggcc gcgcctgcca ggacaagaag caagaacaag aaaaccggcc
    17641 atcaggagga cgtctacgtg acccgaccgt tcgaaggcgt gaagattctt gacttcacgc
    17701 aagtgctggc cggcccctat gcgagctacc agctggcgct gctcggcgcc gacgtcatca
    17761 aggtggagcg gcgcgaaggc gaggacatgc gccgcacccc cctcagccgc gaatgggccg
    17821 atcgcggcct cgcccccggc tggcaggcga tcaacggcaa caaacgcagc ctgacgctcg
    17881 acctgcagaa gcccgaggcg atcaccatcg tcacgcagct cgccgcaagc gcggacgtgg
    17941 tgatggaaaa ttttcgtccc ggtgtgatgg acaagcttgg tataggctac gccgcgctct
    18001 cggctgttaa cccgaggctg atctattgtg cgatctcagg cttcggccag accggcccgg
    18061 aacgatcggg cgcaggctac gacggcaaga tccaggcgat gtcgggcatc atggcgatca
    18121 ccggtcacga gacgaccggg ccaacgcgcg ccggctttgc ggtctgcgac gtgctgtccg
    18181 gcgccaccgc ggcgttcggc gtatcaagtg cgctgttcca gcgcacccac accggcaagg
    18241 ggcagatggt cgacgtctcc atgctcgagg cgacgctggc gttcctgtcg ggacaagtgg
    18301 ccgattacgc cgtggccggc catcgccagc aactgtccgg caaccaggcg gtgagccgca
    18361 ggccgaccgc caacctgttc aaggcgggcg acggctattt gctgctcgcg gtgaacagtg
    18421 agaagcaata ccgggccctg atggccgcac tcggccgcgc cgacacgctg gaagacccgc
    18481 gcttttccga ttggttcgcg cggcagctaa atgaaccagc gctgcgcgcc atcatcgagg
    18541 aagcgctttc gaagaagggg tcgcgcgagt gggagaagac cctcgaggcg gcaggcgcgc
    18601 cctgcgccag catctggaag gtcgaagagg tgatcgatca cccgcagata tcagcgcgcc
    18661 aggccattca ggagatcgat acgccttacg gccgcttgcg cttcgccggt agcggcttcc
    18721 agcttgccca tggcggcggc aggctcgatt cgatggcgcc tgaactcagc gcccacacgg
    18781 acgaggtgct gacctcgctc ggctatgacg cagatgcaat cgcggacctg cgcgcgcgca
    18841 acatcgtctg aacggcggcg gtcgcgacgc aggaaccgca agagcatgat acggaaaagc
    18901 atgccctcgg gcttgccccg agggtggaaa ccggttttcc ctcgcgacaa acgcggcacg
    18961 cgtttgcgcg gagatcatgc tcaaacaaag agatgagacc acgatccgat tcaatctaat
    19021 cggatcatgg tctagcgtcc tttgtagacc gccttgcgct tttccttcac cgctgacctg
    19081 gcctcgtcgg cgtcttcact ttccttgacc tcgtggagtg cgcgctgtgc ccggccggat
    19141 tgctcggacg gtccctgcgc caaaacacct tccgttacaa aacgcttcaa tgtcttgagc
    19201 accagcgggg agaactcggc catttcgcgc gccattttga gcgcaacctg gacctgctgt
    19261 cctgtcggca caacttcgtt gacgaggccg atattgtagg cgcgctgcgg atcgaggata
    19321 cggctcaaca gcatcatttc catcgcaacc ttgtgcggaa tgcgcgccgc cagtccggca
    19381 atcaggccac cggtgaaacc caatcgcgct tcggggtagg aaaacctggc attctccgcg
    19441 gcaaccgtga gatcgcacat catcgagagc acaagagcgc cgccgacaca ccagccgccg
    19501 accgcggaaa taatcggctt gttggtctca aagccgacag ttggaatgca gcgccagagt
    19561 tccggaagat tgccggtatc ggcgccggcg gaaaacgctt cgttgccggc cccggtgagc
    19621 accgcagcct tctgtgtgcc ggagcgctcg aaatcctgaa acgctttttg cagcatcagc
    19681 gccgtctcgg cacaaatcgc gttgcggcgt tccgggcgat tgatcgtgat cagtgtggtt
    19741 ccgtcggcgt tgttctcgac ctggaccttt tgcatggtta ctccctttca acaatccgca
    19801 cggcagacgg agcgagaccg ttggcagctg gacccaaagc gatgctgccg agcggaatgc
    19861 gcgatctaat ccacgtttga gacgtcccgc aatgtcggtt ctttggccgc tgtcggggaa
    19921 aagcggaagc ggtgccgaca tcgtcaaacc gacgcgattg acccgtcgga catgagcata
    19981 gccggacatt ggcccgcaaa tctgctatat aaccttcaat ctccttaagc cgggggtgca
    20041 tcttgggcag cgagcacgta gagcggcgac tggcggctat tctggcggcg gatgtcgcgg
    20101 gctcttgccg cttgataggg atcgacgaag aaggcgcgct ggcgcgactg aaagctctta
    20161 gaaagacgct ttttgatcca aaaatcactg atcatcgcgg gcgcatcgtc aagaataccg
    20221 gggacggcgc tctcgttgag ttcggcagcg tcgtagatgc agtacgctgc gcggtcgaaa
    20281 ttcagcgcgg catggccaaa cacaatatcg atgtaccgca ggtcaagcgg atcgagtttc
    20341 gcatcggcat tcacgtcggt gatatcatca tcgaagacca cgatatcttt ggtgatgggg
    20401 tcaatattgc agtccgtctc gaagggatcg cagaaccggg tggtgttagt atttccgacg
    20461 atgctcatcg gcaaattcgt ggcaaggtag atattacctt tgagaatctg ggttcgcaat
    20521 ctttgaagaa tatcgccgaa ccgatgcggg tctggcgcgt tccaaacggt ctcgttgcgc
    20581 caccagtgcc gaaccgcccg ccggttgatg acgttggacc gcttgccctt cctgacaggc
    20641 cttccattgc ggtgttgccg tttcagaata tgagtggtga tcctgagcag gaatatttcg
    20701 cggacggcat cgtggacgac atcaccacgg cgctctcgcg gttcaagctg gttttcgtca
    20761 tcgcgcgcaa ttcgagtttt gcttacaagg gaaaagctgt cgatatcaag cagatcgggc
    20821 gcgagctggg cgtgcgctac gttctagaag gcagtgttcg caaggcagca gggaaagtcc
    20881 gcatcacagg ccaattgatc gacgccacga ccggggtgca cctttgggcg gatcgatttg
    20941 aaggtgacct gagcgatatt ttcgctctac aggatcagat gacggagggt gtcgtctctg
    21001 ccattgcacc aagaatgttt caaactgaaa tcgatctggc cgtgcgtcgg tcgaacaatc
    21061 tcagcgccta cgacctttgt cttcgagcat catcgcattc caaatcatgg acccggggcg
    21121 gattggccga ggctcttcgg cttgcctctc gcgcattgga gattgaccct cggtgcggtt
    21181 atgctgcgac ccttgcaggg gactgccacc tcggaaatgt acgtcaggga tgggcggccg
    21241 atccgaagtc ggaaattgca gaa
//
LOCUS       DATNOI010000157         3059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_301045,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000157 DATNOI010000000
VERSION     DATNOI010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301045"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VN968_06040"
     CDS             <1..395
                     /locus_tag="VN968_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007698268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL28833.1"
                     /translation="GGAEQDTIDIVLEEFPRLKPLLDRAGGALSGGEQQLLAIARCLC
                     GDPRLVLLDEPTEGIQPSIIDEIVETLQRLRAKSGLTMILVEQNLDFIAALSQRILII
                     QKGTITREVAPGELGDASLVGEFVGIAT"
     gene            436..1950
                     /locus_tag="VN968_06045"
     CDS             436..1950
                     /locus_tag="VN968_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXL28834.1"
                     /translation="MAVRRPTLDQLRSVAEDLGMHMGDAELKSYDALMQANYAAYDAV
                     DAMPDYVPAVKYPRTPGYRPEGEENKYNAWYVKTEIKGAPSGKLAGKKIALKDNISLA
                     GVPMMNGASTLEGYIPDTDATIVTRMLDAGGTIVGKVHCEYFCFSGGSHTCAAGPVHN
                     PHKMGYSAGGSSSGSAVVVATGEADMAIGGDQGGSIRIPAAFSGIYGMKATYGLVPYT
                     GVMPIELTIDHTGPMTNNVRDNALLLEVLAGADGLDPRQGALKIGKYSDELGGGVRGL
                     RIGIVKEGFGHPSSEADVDAKVKAGADLFRKLGATVEEISVPMHLVAPAIWLPIAAEG
                     ATEFMMKGNGMGTNWRGLYNTTLLDAHSAWRHRADELSDSLKITMLLGQYFTKHYRGH
                     FYAKAQNLNRKLRAAYDAAMSNYDLLLMPTLPMKATPLPPPDAPRELYIQRAFEMVPN
                     TAPFNASGHPAMSLPCGMSNGLPVGLMLIGKYFDEPTIYRAAAAFEGAGDWKKM"
     gene            2011..2766
                     /locus_tag="VN968_06050"
     CDS             2011..2766
                     /locus_tag="VN968_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL28835.1"
                     /translation="MDFGLKGKSALVTGGSKGIGLAIAELFAAEGANVAICARNADEV
                     GNVVKSLSAKGVKSWGRAVDVADPDALKGWIDGAAAELGGIDTIVCNVSALAVGDTPE
                     TWEKSFRTDMMHTVNSVAAALPYLEKSKSASIIIVSSVSGFEVDFAAGSYGAFKAALI
                     HYAKGLSNQLIAKGIRVNAVSPGNTYFEGGIWQNIERGMPDLYKTAMSLNPTGRMGTP
                     QEVAAGVVFLASPVATRISGTNLIIDGALTKAV"
BASE COUNT          618 a          933 c          967 g          541 t
ORIGIN      
        1 cgggtggcgc agagcaagac accattgata tcgtgctcga ggagttcccc cgcttgaagc
       61 cgctgctgga tcgcgccggc ggcgcgctgt cgggcggtga acagcaatta ttggcgatcg
      121 cgcgctgcct ttgcggcgac ccacgcctgg tcctgctcga tgagccgacg gagggcattc
      181 agccttccat catcgacgaa atcgtcgaaa cgttgcagcg gctacgggcc aagagcgggc
      241 tcacgatgat cctggtcgaa cagaatctcg atttcatcgc cgcgctctcg cagcgcatcc
      301 tgatcatcca gaaggggacc attaccaggg aagtagcgcc gggcgaactc ggcgatgcaa
      361 gcctggttgg agaatttgtc ggcatcgcaa cgtaaagccc gcttccagcg ggcatgatca
      421 gaggagaccc aagccatggc agtccggaga cccacactcg atcagctacg cagcgtcgcc
      481 gaagatctcg gcatgcacat gggtgacgcg gagctcaaat cctacgacgc tctgatgcag
      541 gcgaactatg ccgcctatga cgcggtcgac gccatgccgg actatgtccc ggcggtaaaa
      601 tatccgcgca cgcccggcta tcgcccggaa ggcgaggaga acaaatacaa cgcctggtac
      661 gtcaagacgg agatcaaggg cgcccccagc ggcaagctcg ccggcaagaa gatagcgctc
      721 aaggacaaca tctcgctcgc cggcgttccc atgatgaacg gtgcctcgac cctcgaaggc
      781 tatatccccg ataccgacgc gaccatcgtc acccgcatgc tggacgccgg gggcaccatc
      841 gtcggcaagg tccattgcga atatttctgc ttctccggcg gcagccacac ctgcgccgcc
      901 ggtcccgtcc acaatccgca caagatgggt tactccgccg gcggctcgtc gtcgggcagc
      961 gccgtcgtgg tggcgaccgg cgaggccgac atggcgatcg gcggcgatca gggcggatcg
     1021 atccgtattc cggcggcctt cagcggcatc tacgggatga aggcgaccta tggcctcgtg
     1081 ccctatacgg gcgtgatgcc gatcgaactc acgatcgacc acacaggccc gatgacgaac
     1141 aatgttcgcg acaacgcgct tctcttggaa gtgctggcgg gcgccgatgg acttgatcca
     1201 aggcaaggcg cgctcaagat cgggaaatat agcgacgaac tcggcggcgg cgtcagggga
     1261 ttgcgcatcg gcatcgtcaa ggaaggcttt gggcatccga gttcggaagc ggacgtcgat
     1321 gccaaggtca aggcgggcgc cgatttgttc cgcaagctcg gcgccactgt cgaggaaatc
     1381 tcggtgccga tgcatctcgt ggcacccgcg atctggttgc cgatcgccgc cgaaggcgcc
     1441 accgagttca tgatgaaggg caacggcatg gggaccaact ggcgcggtct ctacaacacc
     1501 acgctgctcg atgctcactc ggcgtggcgg catcgcgccg acgaattgtc cgacagcctc
     1561 aagatcacga tgctgctcgg tcagtatttc acaaagcact atcgcggtca tttctacgcc
     1621 aaggcacaga atttgaaccg caagctgcgc gctgcctatg acgcggcgat gagcaattac
     1681 gacctgctgt tgatgccgac cttgccgatg aaggcgacgc cgctgccgcc gccggacgcg
     1741 ccacgcgaac tctatatcca gcgcgcgttt gaaatggttc ccaacaccgc gccgttcaac
     1801 gccagcggac atccggcgat gagccttccc tgcggcatga gcaacggtct gccggtcggg
     1861 ctgatgttga tcggcaagta ttttgacgag ccgacgatct atcgtgccgc cgccgcgttc
     1921 gaaggagcgg gcgactggaa gaagatgtaa ccggaccgag gcggcctgcc ggcatgcgag
     1981 caggccgccg caccagcaac ggaggcgacc atggatttcg gattgaaggg taagagcgcg
     2041 ctcgtgaccg gcggcagcaa gggcattggg ctggcgatcg ccgagctgtt tgcggccgag
     2101 ggcgccaacg tcgcgatctg cgcgcgcaat gccgacgagg tcggtaacgt cgtgaagagc
     2161 ctctcggcga agggtgtcaa atcgtggggc agggcggtcg atgtcgccga cccggatgcg
     2221 ctgaagggct ggatcgacgg ggccgccgcc gagctcggcg gcatcgatac tatcgtctgc
     2281 aacgtcagcg cgctggcggt tggcgatacg cccgagacct gggagaaatc gttccgcacc
     2341 gacatgatgc ataccgtcaa ttcggtcgcg gccgcactgc cttacctgga aaagtcgaaa
     2401 tcggcgtcga tcatcatcgt ctccagcgtg tcgggcttcg aggtcgattt cgccgccggt
     2461 tcgtatggcg ccttcaaggc ggccttgatc cactacgcca agggcctcag caatcagctg
     2521 atcgccaagg gcatccgcgt caacgcggtg tcgccgggca acacctactt cgaaggcggc
     2581 atctggcaga atatcgagcg cggcatgccc gatctctaca agacggcgat gtcgctcaat
     2641 ccgaccggac gtatgggcac accgcaggaa gtcgccgccg gcgtggtgtt tcttgcgagc
     2701 ccggtagcga cccgcatctc cggcaccaac ctcatcatcg atggtgcact cacaaaggct
     2761 gtctagcgag ctgtcgaccg gggttcaggt tgcagctcgc ttggcgggaa gacgtggttg
     2821 ctggcgctcg ctcaaggttt ccgccatggc agtttcaacg cgtgatgctt tctgacgctt
     2881 gattgtcgat atgacctcat tagaggttat tggctgaagc tagtgttccg tcactgacaa
     2941 acgattcaca aatcggcgcg aacctgcgag tctgcgccga tgaggaaaat tgagcgaatt
     3001 tcgatcaccg cggccgatcg tgaacgattg gagcggctgg tgcgggaccg aaacacgcc
//
LOCUS       DATNOI010000158         3629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1703959,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000158 DATNOI010000000
VERSION     DATNOI010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703959"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VN968_06055"
     CDS             <1..597
                     /locus_tag="VN968_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HXL28836.1"
                     /translation="QISALRRQLAALEEALQASEKRDRESQGRIADLGQRLNVALAQR
                     VQELSRYRSEFFGRLRAILGNRPDIRIVGDRFVFQSEVFFDTGQALLLPEGRAELDQL
                     ATALIDLDKQIPGEIAWVLRVDGHTDVRPINSPLFKSNWELSSARAISVVQYLISLGV
                     PAQRLVAAGFAEFQPLDTAATEDAYKRNRRIELKLTER"
     gene            597..1046
                     /locus_tag="VN968_06060"
     CDS             597..1046
                     /locus_tag="VN968_06060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL28837.1"
                     /translation="MAAPFHLRPYRSEDEDAAIALWQRSWQQAYPSIDFAARAPWWRE
                     RWRNELVPSAAIIVAEQADTLIGFVTIDLKGYLDQLVVSPTHWGSELGNALVDEAKRL
                     SPDRITLLVNTDNARAIRFYERNGFARAGGDVNPTSDRPVLRMEWMP"
     gene            complement(1119..2978)
                     /locus_tag="VN968_06065"
     CDS             complement(1119..2978)
                     /locus_tag="VN968_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HXL28838.1"
                     /translation="MSAVEPLEDRHGGTPLQAGAEPAEASSIEAQLTQSAASGRARLR
                     PLLALVPYVARYRGRAVLAFIALTVAAVTTLLVPVAVRRMIDFGFTPEGIAMINSYFS
                     VMIAVVAVLAGASAARFYLVMTIGERIVADLRRDVFAHLMSLSPVFFDSARSGELISR
                     LTADTTQIKSAVGASVSIALRNLMLFIGATAMMVITSPRLSGFVLLAIPLIVLPLVAF
                     GRWVRRLSRNAQDTLADATAYASELVGAIRTVQAYTSERLANARFGGEVEQAYEAARS
                     STRARAVLTAIIIFIVFTSVVAILWVGSHDVLTGSISPGRLGQFVLYTAFAAAGLGQL
                     SEVWGEVSAASGAAERLFEILRVKSQIAAPASPRALPVPARGDVGFENVSFAYPTRPD
                     APAVDGVSLSVRAGEKVAIVGPSGAGKSTLFHLLLRFYDPASGTILFDGVPIRAADPR
                     EVRSRIALVPQESVVFATTARENIRFGRPDADDAEVERAAELAHAGEFIRRLPGGFEA
                     QLGERGVTLSGGQRQRIAIARAILRDAPLLLLDEATSSLDAESETLVQTALEELMHHR
                     TTLVIAHRLATVLSCDRIMVMDQGRIVEQGTHASLVAVNGLYARLARLQFEGV"
     gene            3161..3388
                     /gene="rpmE"
                     /locus_tag="VN968_06070"
     CDS             3161..3388
                     /gene="rpmE"
                     /locus_tag="VN968_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HXL28839.1"
                     /translation="MKADIHPNYHMITVVMTDGTEYRTRSTWGKEGDKLNLDIDSKSH
                     PAWIGGAQQILDRGGRVSRFQKKFSGFLKKD"
     gene            complement(3450..>3629)
                     /locus_tag="VN968_06075"
     CDS             complement(3450..>3629)
                     /locus_tag="VN968_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HXL28840.1"
                     /translation="LPQQLQDLIARSMTLQTKVRRLDTTIHAPVTERAAIGNPLVPQL
                     NRLKAAFEQSSLRGK"
BASE COUNT          685 a         1167 c         1187 g          590 t
ORIGIN      
        1 caaatcagcg cgctgcgccg ccagcttgcg gcgcttgagg aggcgctcca ggcctcggag
       61 aagcgcgacc gggaatccca ggggcggatc gccgatctcg gtcagcggtt gaacgtggcg
      121 ctggcgcagc gggttcagga attgtcgcgc taccgttcgg agttttttgg gcgcctgcgc
      181 gccattctcg gcaaccggcc ggacatccgg atcgtcggcg accgcttcgt attccagtcg
      241 gaggtgttct tcgacaccgg acaggcgcta ttgctgcccg aaggccgcgc cgaactcgat
      301 cagctggcga ccgcgctgat cgacttggac aagcagatcc cgggcgaaat cgcctgggtg
      361 ctgcgggtcg acggccatac cgacgtgcgg ccgatcaaca gcccgctgtt caagtcgaac
      421 tgggaattgt cgtcggcgcg cgccatctcg gtcgtgcagt acctgatctc gctcggcgtg
      481 ccggcgcagc ggctggtcgc ggctggattc gcggaattcc agccgcttga cacggcggcg
      541 accgaagacg cctacaagcg caaccgccgc atcgaattga agctgacgga gaggtagtgg
      601 ccgcgccgtt tcacctgcgc ccctatcgga gcgaggacga ggatgccgcg atcgcgctgt
      661 ggcaacgcag ctggcaacag gcctatccgt ctatcgactt cgccgcgcgc gcgccatggt
      721 ggcgcgagcg ctggcgcaac gagctggtgc cgagtgcggc gatcattgtt gcagaacagg
      781 ccgatacgct gatcggattt gtgacgatcg atctcaaagg ttacctcgac caactcgtgg
      841 tcagccctac acattggggc tcggaactcg gcaatgcgct ggtcgacgag gccaagcgct
      901 tgtcgcccga tcggatcacg ctattggtca acaccgacaa tgcccgcgcc atccgcttct
      961 atgagcgcaa cggttttgcg cgggccggcg gcgatgtgaa cccgacgtcg gaccggccgg
     1021 tgctgcggat ggagtggatg ccgtaggtaa ccccgtcatt gcgaggagcg tagcgacgaa
     1081 gcaatccatc ctgccacgga agggaccggc cccgtccctc acaccccctc gaactgcaat
     1141 ctcgccagcc gcgcatagag tccattgacg gcgaccagcg aggcatgggt accctgctcg
     1201 acgatacggc cctgatccat caccatgatg cggtcgcagg acaatacggt ggcgaggcga
     1261 tgggcgatca ccagcgtggt gcggtgatgc atcaattcct ccagtgctgt ctgcaccagc
     1321 gtttcgctct cggcatcgag cgacgatgtc gcctcgtcga gcagcagcag cggcgcgtcg
     1381 cgcaggatcg cacgcgcaat ggcgatgcgc tggcgctgcc ctcccgacag cgtcacgccg
     1441 cgttcgccga gctgcgcctc gaatccgccc ggcaggcggc ggatgaattc gccggcgtga
     1501 gcaagctcgg cggcacgctc gacctcggcg tcatcggcat cgggacggcc gaagcggatg
     1561 ttttcgcgcg cggtcgtggc aaacacgacc gattcctgcg gcaccaatgc gatgcgcgaa
     1621 cgcacctcgc gcggatcggc cgccctgatc gggacgccat cgaataaaat cgtgccggaa
     1681 gcgggatcgt agaagcgcag cagcaggtgg aacagcgtgc tttttcccgc gcccgacggg
     1741 ccgacgatcg cgaccttctc gccggcgcgc accgacagcg aaacgccatc caccgcgggc
     1801 gcatccggcc gcgtcgggta ggcaaagctg acgttttcga agccgacgtc gccgcgcgcc
     1861 ggcaccggca aggcgcgcgg cgatgccggt gccgcgatct gcgatttgac ccgcagaatt
     1921 tcgaacaagc gctcggctgc gccggaggcg gccgaaacct cgccccagac ctcgctgagc
     1981 tggcccaggc ccgccgcggc aaacgccgtg tacaatacga actggccgag ccggccagga
     2041 ctgatggagc cggtcagcac gtcgtgcgat ccgacccaga ggattgcgac gacgctggta
     2101 aagacgatga agatgatgat cgcggtgaga accgcgcggg cgcgggtcga actgcgcgcc
     2161 gcttcatagg cctgctcgac ttcgccgccg aagcgcgcat tggccagccg ctcgctggta
     2221 taggcctgca cggtgcggat ggcgccgacc agttccgacg cataggcggt cgcatccgcc
     2281 agcgtatcct gggcgttgcg cgacaaccgc cgcacccaac ggccgaacgc caccaaaggc
     2341 agcacaatca gcggaattgc caacaggaca aaacccgaaa gccgggggct cgtgatcacc
     2401 atcatcgcgg tggcgccgat gaacagcatc aggttacgca gcgcaatcga taccgaggcg
     2461 ccgaccgctg acttgatctg ggtggtatcg gcggtgaggc gcgagatcag ttcgccgctg
     2521 cgcgcggaat cgaagaatac cggcgacagc gacatcaggt gagcgaacac gtcgcgccgc
     2581 aaatccgcta caatgcgttc gccgatggtc atcaccagat agaaccgcgc cgcgctggcg
     2641 ccggcgagca cggcaacgac cgcgatcatc acgctgaagt agctgttgat catggcgatg
     2701 ccttcggggg tgaatccgaa gtcgatcatc cgccgcaccg cgaccggcac gagcagggtg
     2761 gtgactgcag cgaccgtcag cgcgatgaaa gccagaacag cgcgtccgcg ataacgcgcc
     2821 acatagggga ccagcgccag tagcggacga agccgcgcgc ggccgctcgc cgccgactgc
     2881 gtcagttggg cttcgatgga ggatgcctct gccggctccg caccggcctg gagcggggtt
     2941 ccgccatgcc ggtcttcaag cggttccact gcgctcatga gatctggacc attcgctgct
     3001 gccttcccag ataggccccg ctgccagcgc tggcaaatcc gtaaacacac gattccggga
     3061 ggttactcgg cttgttttag ggggcttgac gcgttataga gcggcccaaa tccgtcacca
     3121 cagccatgga aacacaggcg caggcgccga aggatctgcc atgaaagccg atattcaccc
     3181 gaattatcat atgattacgg tcgtgatgac cgacggcacg gaataccgga cgcgttccac
     3241 ctggggcaag gaaggcgaca agctcaacct cgatatcgac tccaagtcgc acccggcctg
     3301 gatcggcggc gcccagcaga tccttgatcg aggcggccgc gtatcccggt tccagaagaa
     3361 gttctcgggc ttcctcaaga aggattgagg cctctccagc ctctgttcgg cgacagggat
     3421 cagacgcctc ggctcacgcc ggggcgtttt tatttgccgc gcagcgagga ttgctcgaat
     3481 gccgccttca ggcgattgag ctgcggcacc agcggattgc cgatcgcggc gcgttccgtc
     3541 accggggcat ggatggtggt gtcgaggcgg cggactttcg tttgcagtgt catcgagcgg
     3601 gcgatcaagt cctgcaattg ctgcggcag
//
LOCUS       DATNOI010000159         2901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_300073,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000159 DATNOI010000000
VERSION     DATNOI010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300073"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VN968_06080"
     CDS             complement(<1..167)
                     /locus_tag="VN968_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXL28841.1"
                     /translation="MSALANQAFAKMNGIGNEIVVVDMRDKPADVTSEEARAIATPSG
                     VHYDQLMVLQPP"
     gene            385..1131
                     /locus_tag="VN968_06085"
     CDS             385..1131
                     /locus_tag="VN968_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HXL28842.1"
                     /translation="MNRCRFFRHAMVALVPAAAIWLGLGNVLAQTPPPPPAPPAVSTP
                     TPPAPAPAATPAPAQPATPAPGASSPAAPGTPPATAETPATPPPAAAPVQTADPFGEE
                     ITLTPKTVVIMRGNANWDSAFDTLIDSFKALSALLDKQGIKPAGNSMIVYTATDDTGF
                     TYLAEIPVEQEPKNLAKDMSMGKSPEGKALKFVHRGSYDNMDNTYEAITNHLDDKKLE
                     AKDTFIEEYITDPLKTAEDKLVINVYVPLK"
     gene            1128..1844
                     /locus_tag="VN968_06090"
     CDS             1128..1844
                     /locus_tag="VN968_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083310.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HXL28843.1"
                     /translation="MKLMKHRAPLVAAIAAGTLLAAPALAQTVPPAMISVTGEATVSV
                     PPDQAQIDAGVATEAKTAREASDSNNAAMGKVLLALKGAGIEEKDYQTSRLSLQPESA
                     PNRSTGPATIVGYRASNRVTIRLRDVTKVASIIDTLVGAGANDIGGINFTVSQASKLL
                     DDAREQAIADARRKAEIYAKAAGVTLGAPLSISEGGTPGPITFRRMAAGMAAAAPVAQ
                     GEETLQVTVAVSWAIKQAGQ"
     gene            complement(1980..>2901)
                     /locus_tag="VN968_06095"
     CDS             complement(1980..>2901)
                     /locus_tag="VN968_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL28844.1"
                     /translation="DGSPPKSLGEVLRWQFGPARQRQAWPVWVTREHADTPPARVSGD
                     KVRLSFVGHASWLIQTSGLNILIDPVWSARASPVSWAGPKRCNDPGIAFEALPPIDIV
                     LVSHGHYDHLDIVTLSKLATKFSPRVITPLGNDVTMRSANEAIRAEAFDWHDRVELGN
                     GIAVTLVPTRHWSARGLFDRNKALWASFVLETPAGKIYIVCDSGYGEGKHFRRVAQAH
                     GPLRLAILPIGAYEPRWFMQDQHMNPSDAVKALADCGAGQALAHHHGTFQLTDEAIDA
                     PVIALGAALDEAKIPRERFVALKPGQVLEI"
BASE COUNT          561 a          982 c          882 g          476 t
ORIGIN      
        1 ggcggctgca gcaccatcag ctggtcgtaa tgcacgccgc taggggtggc tatcgcgcgg
       61 gcttcctccg acgtgacgtc ggcgggcttg tcgcgcatat cgaccacgac gatctcgttg
      121 ccgatgccgt tcatcttggc aaacgcttgg ttggcgagcg cgctcatcga atttcccgat
      181 tgtgcgtccc ttatatggcg aatgatgccg gattgaccag tgcggcaaat ctgtgtcggc
      241 taccttcgct cgacagggaa gacccatgac gcatctggca tgggacgaaa gccgaccttg
      301 cgtgttagcc tgcatcagtc gccagcggaa tccgggaaga tgctgcgcaa cgccattgct
      361 gaaaagaccg tggagaccgt ccgaatgaat cgctgccgct tttttcgtca tgccatggtc
      421 gcgctggtcc cggcagccgc gatttggctg gggcttggca acgtgctggc gcaaacgccg
      481 cctccgcctc cagctcctcc cgctgtcagt acgccaacgc cgccggcgcc tgccccagcc
      541 gcaactcccg ctccggcgca accggcaacc ccggctccgg gagccagctc cccggccgcg
      601 cctggcactc cgcctgcaac ggctgagacc ccggcgacac cgccgccggc tgccgcgccg
      661 gtccagaccg cggatccgtt cggcgaagaa atcaccctga cgccgaaaac ggttgtcatc
      721 atgaggggca acgccaactg ggattcggcc ttcgacaccc tgatcgattc gttcaaggcg
      781 ctgtcagcac tgctcgacaa gcagggcatc aagccggccg gcaattcgat gatcgtctac
      841 accgcgaccg acgataccgg cttcacgtat cttgcggaaa taccggtcga gcaggagcca
      901 aagaatctgg ctaaggacat gagtatgggc aaatcgcccg agggcaaagc gctgaagttc
      961 gtccatcgcg gctcctacga caatatggac aacacctatg aggccatcac caatcatctc
     1021 gatgacaaga agctcgaagc caaagacacc tttatcgagg aatacatcac cgatcccctg
     1081 aagaccgcgg aggacaaact ggtgatcaac gtctatgtgc cgctgaaatg aagctcatga
     1141 agcaccgcgc tcccctcgtc gccgccatcg ctgcgggcac gctgcttgcc gcgcccgcgc
     1201 tggcgcagac cgtgccgccg gcaatgatct ccgtcaccgg cgaagccacc gtatcggtgc
     1261 cgcccgatca ggcgcagatc gacgccggcg tcgccactga ggccaaaacc gcgcgcgagg
     1321 cgtctgactc caacaacgcg gcgatgggca aggtgctgct ggcgctgaag ggcgccggca
     1381 tcgaggagaa ggattatcag acctcgcggc tgtcgctgca gccggaaagc gcgccgaacc
     1441 gcagcaccgg cccggcgacc atcgtcggct accgggccag caatcgcgtc accatccgcc
     1501 ttcgcgacgt caccaaggtc gcaagcatca tcgatacact ggtgggcgcc ggcgccaacg
     1561 atatcggcgg catcaatttc acggtgtcgc aggcgtcgaa actgctcgat gacgcccgcg
     1621 aacaggcgat cgccgatgcc cgccgcaagg ccgagattta cgccaaggcg gccggcgtca
     1681 cgctcggcgc gccgctcagc atctcggagg gtggtacgcc cggcccgatc acgttccgca
     1741 ggatggcggc cggcatggcc gcggcagcac ctgtggcaca gggcgaagag acgctccagg
     1801 tcacggtagc tgtgtcctgg gcgattaaac aggcggggca gtgatgcttc tagccagaga
     1861 ctcgacctca tcctgaggag cgcgctcttg gcgcgcgtct cgaaggatgg agatgggccc
     1921 tcgtgcttcg agacggcgct aaagcgcctc ctcagcatga gggattacaa acgagcccac
     1981 taaatctcca acacctgtcc cggcttgagc gcgacaaaac gctcgcgcgg aatcttcgct
     2041 tcatcgagcg ccgcacccag cgcaatcacc ggcgcgtcga tcgcctcatc ggtcagttga
     2101 aacgtgccgt gatgatgcgc cagcgcctgt ccggcgccgc aatcggccag cgccttgacc
     2161 gcatccgacg ggttcatgtg ctgatcctgc atgaaccagc gcggctcata ggcgccgatg
     2221 gggaggatcg ccaaccgcag cggaccatgc gcctgcgcca cccgacggaa atgcttgccc
     2281 tcgccatagc cggaatcgca gacgatgtag attttgcccg caggagtctc caggacaaaa
     2341 ctcgcccaca gcgccttgtt gcggtcgaac aacccgcgcg ccgaccagtg ccgcgtcggc
     2401 accagcgtca ccgcgatgcc gttgccaagc tccacccggt catgccaatc gaacgcctcg
     2461 gcgcggatcg cttcattggc gctgcgcatg gtgacatcgt tgccgagcgg ggtgatcacg
     2521 cgcggcgaga atttcgtggc gagttttgac agcgtcacga tgtcgagatg gtcgtaatga
     2581 ccgtgcgaca ccagcacgat gtcgatcgga ggcagcgcct cgaacgcaat gcccgggtcg
     2641 ttgcaacgct tcggcccggc ccagctcacc ggcgacgccc gcgccgacca caccggatcg
     2701 atcaggatgt tcagtccgga cgtctggatc agccagctgg cgtggccgac gaacgacaat
     2761 cgtaccttgt cgccgctgac gcgcgccggt ggcgtatcgg catgctctct cgtcacccac
     2821 acgggccagg cctgccgctg ccttgccgga ccgaactgcc agcgcagcac ttcaccgagc
     2881 gatttcggcg gcgagccgtc g
//
LOCUS       DATNOI010000160         3981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2241972,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000160 DATNOI010000000
VERSION     DATNOI010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2241972"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VN968_06100"
     CDS             <1..1182
                     /locus_tag="VN968_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXL28845.1"
                     /translation="FKAVNDTFGHPVGDKLLKIVADRLRGLVRETDTIARMGGDEFVI
                     VQPFIADPADATALAQRIITLMSEPYSIDGHQAVIGASIGIAVGPGDGSGADKLLRNA
                     DLALYRAKGDGRGTFRFFEPAMDLQMQTRRIMERDLRKALPAGEFELYYQPVVNLANS
                     EISGFEALIRWNHPERGLVSPAAFIPLAEETGFIVPLGEWVIRQACATAAQWPGDLRV
                     AVNISAVQFRNPGLMQVVVGALAASGLDPTRLEIEITETVLLHNKEATLAVLHQLRAL
                     GIRIAMDDFGTGYSSLTYLQCFPFDKIKIDRSFVKDITENSGSLNIVRAVAALARGMG
                     MMATAEGVETREQLDQITSEGCTEMQGFLFSQPIPAHEIERLFLSGYEARNGLGRIVA
                     A"
     gene            complement(1199..1714)
                     /locus_tag="VN968_06105"
     CDS             complement(1199..1714)
                     /locus_tag="VN968_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXL28846.1"
                     /translation="MTVSKTAAVDLAKAARRKDPAEGAGESPALQMGELSELLGYSLK
                     RAQLKVFEDFLRCVAPLQLTPAQFSVLLLVDKNPGRNQTEIAVTLGILRPNFVAMLDG
                     LESRDLCVRMRSTNDRRSHILVLTDKGRAVLARAKKLVATKHEARLNELLGPANRAAL
                     LEMLATIAREF"
     gene            2001..3041
                     /locus_tag="VN968_06110"
     CDS             2001..3041
                     /locus_tag="VN968_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL28847.1"
                     /translation="MNTTIMLFLVQDGITNGAIYALLGLALVLVFAVTRVILIPQGEF
                     VTYGALTYATLSAGQVPGTARLAVAMGLVAFGLDLFVGRRSLHPRLVGRAFAVNVMLP
                     VAILALTWWLAGQRTPIAINIALSLLIVAAIGLFLYRIAFQPIAHTSVLVLLIASVGC
                     HLALQGFGLVFFGAEGQRGPTLSDAAFSAGPLRFSGQSIAVYAITVAFIIGLWLLFGF
                     TLTGKALRATAVNRLGARLVGIRTTLSGQIAFLLASVIGAISGILIVPITTLYYDTGF
                     LIGLKGFIAAIIGGLVSYPLTAVAALVVGSVEAFSSFYASNFKEVIVFFLILPVLVLR
                     SLAAPAVEEEKD"
     gene            3047..>3981
                     /locus_tag="VN968_06115"
     CDS             3047..>3981
                     /locus_tag="VN968_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL28848.1"
                     /translation="MMQRLPVIVFAAVMAAIPFIPGMPPFWIVLLDNIGLAALVAMGL
                     VLLTGVGGLTSFGQAAFCGFGAYTTAVLTTAYGVSPWLTLPLSLLVSGIAAVVLGLVT
                     VRLSGHYLPLGTIAWGIGLFYLFSKLEFLGRNDGISGIPPLTIGSLRMIDPGTIYFAI
                     WIAVLISALLTMNLLDSRTGRAIRALRRGHIAAEAFGVQTPRAKLLVFIYAAVLAGLS
                     GWLYAHFQRAANPTPFGAQAGIEYLFIAVVGGAGYVWGGVLGAAIVVILKEILQSYLP
                     YLFHGEGQLETIVFGILLVVLLQLAPTGVWPWLMAR"
BASE COUNT          640 a         1257 c         1232 g          852 t
ORIGIN      
        1 ttcaaggccg tgaacgatac gtttggtcat cctgtcggtg acaagctcct gaaaatagtc
       61 gcggaccggt tgcgcggcct ggttcgcgag accgacacga tcgctcggat gggcggcgat
      121 gaattcgtga tcgtgcagcc cttcatcgca gatcccgccg acgcaaccgc tcttgcgcaa
      181 cgtatcatca cgctgatgag cgagccctac agcatcgacg gccaccaggc cgtcatcggc
      241 gccagcatcg gcattgcggt cggtcccggc gacggatcgg gtgctgacaa gctgctgcgc
      301 aatgccgacc tcgcgctata ccgggcgaaa ggcgatggac gcggcacgtt ccggtttttc
      361 gagcccgcga tggacctgca aatgcaaacc cgtcgcatca tggaacggga cctgcgcaag
      421 gcgctgcccg ccggcgagtt cgaactctac taccagccgg ttgtcaatct ggcgaacagc
      481 gaaatcagcg gattcgaggc cctgatccgc tggaaccatc cggaacgtgg attagtttct
      541 cctgccgcat tcattccgtt ggcggaagag accggcttta tcgtcccgtt gggagaatgg
      601 gtcatccggc aagcctgtgc caccgcggcc caatggccag gagatctccg tgtcgcggtc
      661 aatatttcag cggttcaatt ccgcaatccc ggcctgatgc aggtcgtcgt tggggcactc
      721 gctgcctcgg gcctggaccc gacacggctg gagatcgaaa ttaccgagac cgtcctgctg
      781 cacaacaagg aagcgacact tgccgtgctt caccagttgc gagcacttgg catccggatc
      841 gcgatggacg attttggcac cggatattcc tcgcttacct atctccagtg ctttccgttc
      901 gacaagatca agattgaccg ctcgttcgta aaggacatta cggaaaactc gggctcgctc
      961 aatatcgtgc gggcggtcgc ggcgctcgca cgcggaatgg gcatgatggc cactgccgaa
     1021 ggcgtcgaaa ccagagaaca gctcgaccag atcacgtccg aaggctgcac cgaaatgcag
     1081 ggattcctgt tcagtcagcc gatccccgcg catgagatcg aacggctgtt cctttcaggg
     1141 tacgaggcgc ggaacggtct tggccgtatt gtcgcagctt gatcgctaat ttatagacct
     1201 aaaactctct tgcgatcgtc gccagcattt ccagcaacgc ggcgcggttg gcggggccga
     1261 gcaactcgtt gagacgcgct tcgtgcttgg ttgcgacgag cttcttggcg cgcgccagca
     1321 ccgcgcggcc cttgtcggtc aggacaagaa tgtgggagcg gcgatcgttg gtcgaacgca
     1381 tccgcacgca caggtcgcgg ctctccaggc cgtcaagcat cgccacgaaa ttcggccgga
     1441 ggatgccgag cgtaacggcg atctcggtct ggttacgtcc cggattcttg tcgaccagca
     1501 gcagcacgga aaattgcgcc ggggtaagct gcagcggcgc gacgcagcgc agaaaatcct
     1561 cgaagacctt caattgcgcg cgcttgagcg aatagccgag caactcggac aattcgccca
     1621 tctgcagcgc cgggctttcg ccagcacctt cggcagggtc tttgcggcga gccgccttgg
     1681 cgagatccac ggcggcggtc ttggaaacag tcatcgctca aagctcgcaa tcgcgctagg
     1741 gtccgatcca gcctcactga atcgggcccg tcggttatct ctttgcctgg gcatgatctc
     1801 ttccgaaaac cggttcccct ttttcgggat catgccacag gcctgcaacg gcccggcagg
     1861 cgttacgggc ttgagattat aattgataat tgttatccac cataccaaat atcgacagct
     1921 tttttcaatg gctgtgatcg acgggccggc atcctttttg ggacgcgatc ggcccgacgc
     1981 gtgaggggga gcgcccggtc ttgaacacga cgatcatgct gttcctggtc caggacggca
     2041 ttaccaacgg cgcgatctat gcgctgctcg gccttgcgct ggtgctggtg ttcgccgtca
     2101 cgcgcgtcat cctgattccg cagggcgagt tcgtcaccta cggcgcgctg acctacgcca
     2161 cgctgtcggc cggccaggtg ccgggcaccg cgcggctggc ggtggcgatg ggcctggtgg
     2221 catttggcct cgatttgttc gtcggccgca ggtcgctgca ccccaggctg gtcggacgtg
     2281 ctttcgcggt caacgtcatg ctgcctgtgg cgatcctggc cctgacctgg tggctcgccg
     2341 gccagaggac cccgatcgcc atcaacatcg cgctgtcgct attgatcgtc gcggcgatcg
     2401 ggctgtttct ctaccgcatc gcgtttcagc cgattgcgca cacctcggtg ctggtgctcc
     2461 tgatcgcctc ggtaggctgc catctcgcgc tgcagggttt tgggctggta ttcttcggcg
     2521 ccgaaggcca acgcggcccg acgctatcgg atgcggcgtt cagcgccggc cccttgcgct
     2581 tcagcggcca gagcatcgcc gtctacgcca ttaccgtcgc cttcattatc ggcctgtggc
     2641 tgttgttcgg ttttaccctc accggcaagg cgctgcgcgc caccgcggtc aatcgcctgg
     2701 gcgcgcgcct ggtcggcatc cgaaccacgc tgtcgggcca gatcgcgttc ctgctcgcct
     2761 ccgtgatcgg ggcgatctcg ggaatcctga tcgtgccgat cacgacgctg tattatgaca
     2821 ccggcttcct gatcggcctc aagggattca tcgctgccat catcggcggt ctcgtgagct
     2881 acccgttgac cgcggtcgcg gccttggtgg tcggatcggt cgaggctttc tcctcgttct
     2941 atgccagcaa cttcaaggag gtcatcgtct ttttcctgat ccttccggtg ctcgtcttgc
     3001 ggtcgctggc agcgcccgcg gtcgaggaag agaaggattg atcgcaatga tgcagcggct
     3061 gcccgtcatc gtcttcgcgg ctgtgatggc ggctattccg ttcatcccgg gcatgccgcc
     3121 gttctggatc gtgctgctcg acaatatcgg gctcgccgca ctggtggcga tggggcttgt
     3181 gctcctgacc ggcgtcggcg gcctcacctc gttcggccag gccgcgtttt gcgggtttgg
     3241 cgcctatacg acggcggtgc tcaccaccgc ctatggcgtg tcaccctggc tgacattgcc
     3301 gttgtcgctg ctggtctcag gcattgccgc ggtcgtgctc ggcctcgtca cggtgcggct
     3361 gtcgggacat tatctgccgc tcggcaccat cgcctggggc atcggcctgt tctatctgtt
     3421 cagcaagctc gaattcctcg gccgcaatga cggcatctcc ggcattccgc cgctgacgat
     3481 cgggtcgctc aggatgatcg accccggcac gatctatttt gcgatctgga tcgcggtgct
     3541 gatctcggcg ctgctcacca tgaacctgct ggactcgcgc accggccgcg ccatccgcgc
     3601 gctgcggcgg ggacatatcg ccgccgaagc cttcggcgtg cagacgccgc gggccaagct
     3661 gttggttttc atctatgcgg cggtgctggc cggcctgtcg ggctggctct atgcgcattt
     3721 ccagcgcgcc gccaatccga cgccgttcgg cgcgcaggcc ggcatcgagt atctcttcat
     3781 cgccgtggtc ggcggcgccg gctatgtctg gggcggcgtg cttggcgcgg ccatcgtcgt
     3841 catcctgaag gagattctgc aaagctatct gccatatctg tttcatggcg aaggccagct
     3901 cgagaccatc gtgttcggca tcctgctggt ggtgctgctg caactggcgc cgaccggcgt
     3961 ctggccctgg ctgatggcgc g
//
LOCUS       DATNOI010000161         3316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1595761,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000161 DATNOI010000000
VERSION     DATNOI010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595761"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VN968_06120"
     CDS             <1..498
                     /locus_tag="VN968_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXL28849.1"
                     /translation="DVVLGLEASYLHGGFGGSPPATATTRSSTSPLSDGYFHQVTTTS
                     QAAISITDMATFRARAGYAYGCFLPYLFGGFALGNADITRSATVLDVTTPGPTNPPAL
                     PPTFGSAAEAQHNHLIYGYSAGLGVDINLIGGLFLRAEWEYVRFTASVDTNINTVRAG
                     LGYKF"
     gene            598..1188
                     /locus_tag="VN968_06125"
     CDS             598..1188
                     /locus_tag="VN968_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXL28850.1"
                     /translation="MKIYGDSNSGNCLKVKWVCDRLALPYTWIAVDTLKRETRTTQFL
                     KLNSAGQVPTVEFDDGRVLAESNAIIRYLARGSDLIPADAFAAAKMDEWLFWEQYSHE
                     PYVAVCRFQMVYLGKASSDLDPDKIKRGYAALARMEHQLAATRFLVGDAFSLADVALL
                     AYTRLAHEGGFHLDGYAAVRRWIGEAERSLGLPPAR"
     gene            1205..1675
                     /locus_tag="VN968_06130"
     CDS             1205..1675
                     /locus_tag="VN968_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL28851.1"
                     /translation="MAGNSSITIRRARRDDVLVIVTMLADDPLGSARERLEDPLPPSY
                     FHAFEALANDPHIQLVVAEDGAGAVVGCLQLCILPGLSSQGASRGLIEDVRVATHCRS
                     RGIGERMVQWAIAEARARGCKLVELLTHNTRVDAQRFYARLGFQPSHVGMTLRF"
     gene            1854..2078
                     /locus_tag="VN968_06135"
     CDS             1854..2078
                     /locus_tag="VN968_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28852.1"
                     /translation="MKNYSIVRIGNDYVVQANEKSVLKISSRRKAARLVTDAAELLDS
                     QSPPLAPPDADAEPSTARDPSVIPDPSKVP"
     gene            2157..>3316
                     /locus_tag="VN968_06140"
     CDS             2157..>3316
                     /locus_tag="VN968_06140"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HXL28853.1"
                     /translation="MTAAKPALRPNVPHFSSGPCAKRPGWNAQNLKDAALGRSHRAKI
                     GKAKLRLAIELTREVLEVPAGYRIGIVPASDTGAVEMSLWSLLGARPVTTIAWESFGE
                     GWVGDIVKELKLKDVTKLHAGYGEIPDLSKADPASDIVFTWNGTTSGVRVPNADWISA
                     KREGLTICDATSAAFAQALDWPKLDVVTFSWQKALGGEAAHGMLVLSPRAVERLESYK
                     PAWPLPKIFRMTKGGKLNEGIFEGETINTPSMLCVEDYLDALNWAKSIGGLQALIARA
                     DANTKVLADWKAKTPWIDFLAKDPAIRSNTSVCMKVVDPAITALNPDAQADFAKRLVA
                     LVEKEGAGFDFAHYRDAPAGLRIWCGATVEAKDVALLTQWIDWAFAETKAALP"
BASE COUNT          617 a         1052 c         1026 g          621 t
ORIGIN      
        1 gacgtcgtcc ttggactgga ggcgagttat ttgcatggcg ggtttggagg ctcgcctccc
       61 gcgactgcga cgacccgctc cagcacgtca cccctgtccg atggctactt tcaccaagtc
      121 acaacaacct cgcaggccgc aatctcgatc acggacatgg cgacattccg tgctcgcgcg
      181 ggttacgcct atggatgctt cctgccatac ctgttcggtg ggtttgcgct cggcaatgcc
      241 gatatcacgc gcagcgcaac cgtcctggat gtcacaaccc ccgggccgac aaaccctccg
      301 gcactgcccc caactttcgg gagcgcggcg gaggcccagc ataatcatct gatttatggc
      361 tattccgcag gcctcggcgt cgacatcaat ctgatcggcg gtctgttcct gcgtgcggag
      421 tgggaatacg tccgcttcac ggcttcggtc gataccaaca tcaacaccgt ccgtgcgggt
      481 ctcggctaca agttctaacc gggcgcgccg gcgaaacctc gcgcgccgcc agatactgtt
      541 gacagaattg atcccggctg ttggtctgtc ggtccggcga ggacggtgac agcggccatg
      601 aaaatctacg gcgatagcaa ttcgggcaat tgcctgaagg tgaaatgggt ctgcgacaga
      661 ctggcgctgc cctacacatg gatcgccgtc gacacgctca agcgcgagac ccgcaccacg
      721 cagtttctca agctcaacag cgcgggccag gtaccgacgg tcgagttcga cgatggccgc
      781 gtgctggccg aatccaatgc catcattcgc tatctcgccc gcggcagcga tctcatcccc
      841 gccgacgcgt ttgcggccgc gaagatggac gagtggctgt tctgggaaca atacagccac
      901 gagccctatg tcgcggtatg ccgcttccag atggtgtatc tcggcaaagc ctcgtccgac
      961 ctcgatcccg acaagatcaa gcgcggctac gctgcgctgg cgcggatgga gcaccagctt
     1021 gccgcgaccc ggtttctggt cggcgacgcc ttctccctcg ccgacgtcgc gctgctggcc
     1081 tatacgaggc tggcgcatga gggcggtttc cacctcgacg gctatgccgc cgtgcggcgc
     1141 tggataggcg aggccgaacg gtctcttggc ttgccgccgg cgcgctgagg tctactggat
     1201 tgtcatggcc gggaattcct cgattaccat ccggcgcgcg cgccgcgacg acgtcttggt
     1261 catcgtgacg atgctggcgg acgatccgct cggcagcgca cgcgagcggc ttgaagaccc
     1321 actgccgccg tcgtatttcc acgcgttcga agccctcgcc aacgatccac acatccagct
     1381 tgtggtagcg gaggatggtg caggcgccgt cgtcggctgc ctgcagctct gcattctgcc
     1441 ggggctaagc tcgcagggcg cctcgcgcgg cctgatcgag gatgttcgcg tcgccacgca
     1501 ttgccgcagc cgcggcatcg gagagcgcat ggtgcaatgg gccatcgcgg aagcgcgcgc
     1561 caggggctgc aagctggtcg aactgttgac gcataacacc cgcgtcgacg cgcagcgatt
     1621 ctacgcgcga ttgggatttc agccgagcca tgtcgggatg acgctgcgat tttgatacag
     1681 ccaatcgtgg gcaccgaccc gggcggcatt gcgtccattg cgaaacgtgt tttgccattc
     1741 gtttcggtta acgcgcgata aactaactcg tccttagtaa tattttttgc ggagcgatcc
     1801 gtgacaacct gacgtatccc gtgaggcggc tcgggcagct ttggggaccc cagatgaaaa
     1861 attattcgat tgtcaggatc ggcaacgatt acgtcgtgca ggcgaacgaa aagagcgtct
     1921 tgaaaatctc cagcaggaga aaggctgcca ggcttgtcac cgacgcggcc gaactattgg
     1981 attcgcaatc gcccccgctg gcgccgccgg acgcggacgc cgagccatca actgcccgtg
     2041 atcccagcgt catacctgat ccatccaaag ttccttgacg atctaagctg attcccttat
     2101 gcaccgcggc gggaaacctc tccccaccga gaggcagcta tctggaagga tagatcatga
     2161 ctgcagcgaa gcctgctttg cggccgaatg tgccgcattt ttcgtccggc ccctgtgcca
     2221 agcgccccgg ctggaacgcc caaaatctca aagatgccgc cctcggccgt tcgcaccgcg
     2281 cgaaaatcgg caaggccaag ctcaggctcg cgatcgagct gacgcgcgaa gtgcttgaag
     2341 tgccagccgg ttacaggatc ggcatcgtgc cggcgtccga caccggcgcg gtcgaaatgt
     2401 cgctatggtc gctgcttggc gcgcggccgg tgaccacgat cgcctgggaa tccttcggcg
     2461 aaggctgggt cggcgatatc gtcaaggagc tcaagctcaa ggatgttacc aagctgcacg
     2521 ccggctatgg cgaaattccc gatctgagca aggccgatcc tgcttccgac atcgtcttca
     2581 cctggaacgg caccacctcg ggcgtccgcg tccccaatgc cgactggatc agcgcgaagc
     2641 gcgaaggcct caccatctgc gatgccacct cggcggcgtt cgcgcaggcg ctcgactggc
     2701 ccaagctcga cgtggtgacg ttctcctggc agaaggcgct gggcggcgag gccgcgcatg
     2761 gcatgctggt gctctcaccg cgcgcggtgg agcggctcga gagctacaag ccggcatggc
     2821 cgctgccgaa aatcttccgc atgaccaagg gcggcaagct caacgaaggt atcttcgagg
     2881 gcgaaaccat caacacgccg tcgatgctgt gcgtcgagga ttatctcgac gcgctgaact
     2941 gggcgaaatc gattggcggc ctccaagccc tgattgcgcg cgccgacgcc aacaccaagg
     3001 tgctcgccga ctggaaggcg aaaacgccgt ggatcgattt cctggcgaag gacccggcga
     3061 tccgttccaa tacctcggtg tgcatgaagg tggtcgatcc cgcgatcacc gcgcttaacc
     3121 cggacgcgca agccgacttc gccaagcggt tggtggcctt ggtggagaag gaaggcgcgg
     3181 gtttcgattt cgcgcattat cgcgacgcgc cggcgggtct tcgcatctgg tgcggcgcca
     3241 cggtggaggc aaaggacgtc gcgctgctga cgcagtggat cgactgggcg ttcgccgaga
     3301 ccaaggctgc gcttcc
//
LOCUS       DATNOI010000162         5309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_640083,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000162 DATNOI010000000
VERSION     DATNOI010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640083"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VN968_06145"
     CDS             <1..778
                     /locus_tag="VN968_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXL28854.1"
                     /translation="VVIIVPGSSGVGNSAIEHAAALTSADIAVLVVDPFGARSVTSTV
                     ANQGQFSFAASSYDVLAAAKYLSVLPEIDRAHIGALGYSRGGTAVIQAAVSPLANAVL
                     GQGISLRAVAAAWPWCGYQFEQPDTRPSAIRFFVGDLDMWASPTQCQAYAALMKDRNA
                     DVSIRVFRNAVHGFGNGQQLRNEPDAMTALNAPITYINYRGEILDPYTGSPLPGIDPG
                     ALAKRSAPFLSRGARVGSQDDQAQQFVTDMVGFFQRQLAP"
     gene            complement(840..1454)
                     /locus_tag="VN968_06150"
     CDS             complement(840..1454)
                     /locus_tag="VN968_06150"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL28855.1"
                     /translation="MSETKDRLLRAGEKLFRAQGYSGTGLKQLAAEAQAPWSSMYHFF
                     PNGKEQLAEEVIRYGGDLYEQMIRQCFAAYPDPLEAVGAMFTGEAKILRSSGFRNGCP
                     VASVALDVASTTDRVRKPCADVFASWIRALAEGIAASGIPEEVATDLASYILASLEGA
                     IILSRTSRSVRPLEQTAQFVLHTLEDQLPRRMRKARNARPSVKS"
     gene            1441..2127
                     /locus_tag="VN968_06155"
     CDS             1441..2127
                     /locus_tag="VN968_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28856.1"
                     /translation="MVSDIESIPFAAGQHIAGLAAHYRSITGIAKQKSVLALNVLIYT
                     VRQGAVAAVALVKRSPASHLSQEMTMHNRVLFAALCGGLAAGTLDIGVAALLNSVSPL
                     VVLVFIASGVLGKAAYHAGAAGLILGLALQWAMSFAIALIYGLGCERAEVLQHSWVLG
                     GVLYGLVIFLVMSFAVVPLSRAVPQPAFALPALVENLLAMILFGLIVAFCAHRFGARP
                     QRSLTLTSAG"
     gene            complement(2169..3092)
                     /locus_tag="VN968_06160"
     CDS             complement(2169..3092)
                     /locus_tag="VN968_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXL28857.1"
                     /translation="MNRKVIITCAVTGAIHTPSMSKALPVTPQEIADAAVDAAEAGAA
                     IVHLHARNPKTGQPDQSPEAFAPFLKIIKQRSNCVINLTTGGAPTMTVDERVRPAAVY
                     KPEVASLNMGSMNFAFFGMLNRFKKFDHDWERKHLENKDIVFRNTFQDIEYVLKTLSE
                     TGTRFEFECYDTAHLYNLNYFLEQGMVKPPLFVQTVFGLQGGTGAHPEDVLHMKRTAD
                     RLFGDKMVWSVLGAGRNQLPIAAMAAAMGGNIRVGLEDSLWAAPGRMAASNAEQVTLA
                     RKIIEGLGLEVATSDEAREILDLKGGDKLEV"
     gene            complement(3138..4082)
                     /locus_tag="VN968_06165"
     CDS             complement(3138..4082)
                     /locus_tag="VN968_06165"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HXL28858.1"
                     /translation="MASPDRHVAIVGTGLIGRAWAAIFARAGWKVRLSDPHIPTLKAA
                     PRLIRDELRALARHGLADDPDGAVARISVAGSLREAVMDVEFVQENGPERVEDKQAIF
                     AQLDRLAPPDALLVSSTSAITASRFTETLPGRARCLVGHPVNPPHLVPLVELCGAPWT
                     SADAINRARTIYREIGQVPVTINREINGFVLNRLQGALLAEAFRLVGEGFISAEDLDH
                     TMKDGLGLRWSFLGPFETIELNAPGGIPDYCGRYTGFYKELASAAAGPEVYQSPNVDR
                     VIAAWPHRPTPERLAALTQRRNDRLAALAAHKARPHDK"
     gene            complement(4104..>5309)
                     /locus_tag="VN968_06170"
     CDS             complement(4104..>5309)
                     /locus_tag="VN968_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL28859.1"
                     /translation="AENIRLAVQARHPRRFDPLTNALSIDAINAETDATIRYLGLAGI
                     EKAEAGMLSYGGQRLLDMGVALATAPRILLLDEPLAGLAAAERERIGAIIKRISSDLP
                     VLLVEHDIDRVFQLADRVTVMNEGRVLLDGSVEDARASPKVQEVYIGSGATEVAARPR
                     ETAARVNALLTATHVDTFYGKSHILNNVNFSLHENEIIALLGRNGAGKSTLLKTLIGI
                     APASNGSIRLADSELIGLSSAQTARLGIGYVPQGRGLFAGMSVEQNLELGALKRQTGN
                     GVHWTRQRIYEYFPRIRERLDSPADYLSGGEQQMVAVARALSGDVRVLLLDEPFEGLA
                     PTVVEQLFETFDRLRKEIAIIIVDHNLDLALALSDTTVALERGQVIHQGPSKALRDDL
                     DLRRKVLWL"
BASE COUNT          939 a         1620 c         1682 g         1068 t
ORIGIN      
        1 ggttgtgatc atcgtgccgg gatcaagcgg ggtaggaaat tccgcgatcg agcatgccgc
       61 ggccctgaca tctgccgata tcgctgtttt ggtggtcgac ccattcggcg ctcgttcggt
      121 gacgagcacg gtcgccaatc agggacaatt ctcctttgcc gcgtcgagct acgacgtgct
      181 ggccgctgcc aaatacctgt cggtgttacc cgagatcgac cgcgcgcaca tcggagcgtt
      241 gggatatagc cgcggaggaa cggcggtgat ccaggccgcg gtgtcgccgc tggcgaacgc
      301 cgtgctgggg cagggaattt cgctgcgggc agtcgcggcg gcgtggccat ggtgcggcta
      361 tcagtttgaa cagccggaca ccaggccgag cgcgatacgc ttcttcgtgg gcgatctgga
      421 tatgtgggcc tctcccaccc aatgccaggc ctacgccgcg ctgatgaagg atcgaaatgc
      481 tgacgtatcc atcagggttt tcagaaacgc cgtgcacggg ttcggaaacg gccagcagct
      541 aagaaacgag cccgacgcaa tgacggcgct caacgcacct attacctata tcaactatcg
      601 aggggagatc ctggatccat ataccggatc gcctctgccg ggcatcgacc cgggcgccct
      661 ggcaaagcga agcgcgccgt tcctgtcgcg cggtgccaga gtgggtagtc aggacgatca
      721 ggctcaacag ttcgtcacgg atatggtcgg ctttttccag cggcagcttg cgccctagca
      781 aagaggaatc ttccgcgaat ctcacctgac acggcgagca ccggcccggc aaattggggt
      841 tacgatttca cgcttgggcg cgcgttcctt gccttgcgca tgcggcgggg caattggtct
      901 tcgagcgtgt gcaatacgaa ctgggcggtc tgttcgagag gcctgacact tctggatgtg
      961 cggctcagga tgatagctcc ttccagagag gccaggatat agctcgccag atccgtcgcc
     1021 acttcctccg gtattccgga cgcggctatt ccctcggcca acgcacgtat ccacgaggca
     1081 aaaacgtcgg cgcacggctt gcggacccta tcggtcgtgc tggcgacgtc gagcgcaacc
     1141 gaagcaaccg gacagccgtt tcgaaacccc gatgaccgaa gaatcttggc ctcgccggtg
     1201 aacatcgcgc cgacggcctc aagagggtct ggatatgccg cgaaacattg ccggatcatc
     1261 tgttcataca aatcgccgcc ataccggata acctcttcgg cgagttgctc ctttccgttc
     1321 gggaagaagt gatacatcga actccagggc gcctgggctt ccgccgccag ctgcttgagg
     1381 ccggtgccgg aatagccctg agctcgaaac agcttttcgc cggcgcgcaa taatcgatcc
     1441 ttggtctcag acatcgagtc cattcctttt gcagccggtc aacacatcgc aggtcttgcg
     1501 gcgcattaca gatctatcac agggatcgcc aaacaaaagt ctgttttggc attgaatgta
     1561 ttgatctata cagtccgtca gggcgcggtg gcggcggtcg ctttggtgaa acggtccccg
     1621 gcctctcatt tatcgcagga gatgacgatg cataatcggg tgttgtttgc cgccttgtgc
     1681 gggggtctgg cggccggaac gcttgatatc ggcgtggcgg cgcttttgaa ttcagtcagt
     1741 ccgctggtcg tgctggtttt catcgcgagc ggagttttgg gcaaggctgc ctatcacgca
     1801 ggggcggccg gattgatact ggggctcgcg ctgcaatggg cgatgtcgtt cgcgatcgca
     1861 ttgatttacg ggcttggatg cgagcgggcg gaagtgctgc agcattcgtg ggtcttgggc
     1921 ggggttctgt acggccttgt gatcttcctt gtcatgagct ttgccgtcgt cccgctttcg
     1981 agagccgtgc cgcagcctgc atttgcgctt ccagcgttgg tggaaaatct gctggcaatg
     2041 atcctgttcg gcttgatcgt ggctttctgc gcgcatcggt tcggcgcgcg gccgcaacgt
     2101 tcattgactt tgacatccgc cggataacgg gagagggcgg cccggatgtg acgggccgcc
     2161 attgaccgtc aaacttccag cttgtcgccg cccttcagat cgagaatttc gcgcgcctcg
     2221 tccgatgtcg ctacctcgag gcccaggcct tcgatgatct tgcgggcgag cgttacctgc
     2281 tcggcgttcg acgctgccat ccggccgggc gccgcccaca gcgaatcttc caggccgacc
     2341 cggatattgc cgcccatggc ggctgccatc gccgcgatgg ggagttgatt gcgcccggcg
     2401 ccgagcaccg accagaccat tttgtcgccg aacaggcgat cggcggtgcg tttcatgtgc
     2461 agcacgtcct ccggatgggc gccggtgccg ccctgcaggc cgaacactgt ttgcacgaac
     2521 agcggcggct tcaccatgcc ctgttcgagg aagtaattca gattgtagag gtgcgcggtg
     2581 tcgtagcatt cgaactcgaa gcgcgtgccg gtctccgaca gggtcttcaa gacgtattcg
     2641 atgtcctgga aggtgttgcg gaacacgata tccttgttct cgagatgctt gcgttcccaa
     2701 tcatggtcga acttcttgaa tcgattcagc atgccgaaaa aggcaaaatt catcgagccc
     2761 atgttgaggg aggcgacttc cggcttgtag accgcggcgg ggcggacccg ctcgtcaacc
     2821 gtcatggtcg gcgcgccgcc cgtggtgaga ttgatcacgc agttggagcg ctgcttgatg
     2881 attttcagga acggcgcaaa ggcttcggga ctctggtcgg gctgaccggt cttcgggttg
     2941 cgggcatgca gatgcacgat ggcggcaccc gcctccgcgg cgtcgaccgc ggcgtcggcg
     3001 atttcctgcg gcgtcaccgg cagcgccttc gacatcgagg gggtgtggat cgcgccggtc
     3061 acagcgcagg tgatgatgac tttgcggttc atgttttgct ctccttgttg tgcggcgaac
     3121 cttcaggatt tgtcgtttta cttgtcgtgc ggcctggcct tgtgcgcggc cagcgccgcg
     3181 agacggtcgt tgcgccgctg cgtcagcgca gctaaacgtt cgggcgtagg ccgatgcggc
     3241 cacgccgcga tcacccgatc cacattcggg ctttggtaga cctcgggacc ggcggcagcc
     3301 gacgcaagct ctttgtaaaa gccagtatag cgcccgcaat agtcgggaat gccaccgggc
     3361 gcattgagct cgatggtctc gaacggtccg agaaacgacc agcgcagccc gaggccgtcc
     3421 ttcatggtgt gatcgagatc ctccgccgaa ataaaacctt cgccgacgag tctaaaagct
     3481 tcggctagca gcgcaccctg cagccggttc agcacaaaac cgttgatctc gcggttgatg
     3541 gtgaccggca cctgcccaat ctcgcgatag attgttcgcg cacggttgat tgcatcggcc
     3601 gaggtccacg gcgcgccgca gagttcgacc agcggcacca gatgcggcgg attgacggga
     3661 tggccgacca gacagcgcgc ccggccgggg agcgtctccg tgaaccgcga ggcggtgatg
     3721 gccgaggtcg aggagaccag cagcgcgtcc ggcggcgcca gcctgtcgag ctgggcaaag
     3781 atcgcctgct tgtcttcgac cctctccggt ccgttttcct ggacgaattc gacgtccatc
     3841 accgcctctc tgaggctccc cgcaacggag atccgtgcca ccgcgccatc gggatcatcg
     3901 gccaatccgt ggcgcgccag cgcgcgcaat tcatcgcgga tcaggcgggg cgccgccttt
     3961 agcgtcggga tatgcggatc gctcagccgc accttccatc cggcacgggc aaagatcgcg
     4021 gcccaggccc gtccgatcag gccggtgccg acgatggcga cgtgtcgatc agggcttgcc
     4081 attttcgttt ctctccttcc ttctcacagc cagagcacct tgcggcgcag atcgagatcg
     4141 tcgcgcagtg ccttggacgg cccctggtgg atcacctggc cgcgctccag cgccaccgtg
     4201 gtgtccgaca acgccagcgc caggtcgaga ttgtgatcga cgatgatgat ggcgatttcc
     4261 ttgcgcaggc gatcgaaggt ttcgaacaat tgttcgacca cggtcggcgc cagaccctcg
     4321 aaaggctcat cgagcagcaa tacccggacg tcgccggaca gcgcgcgggc gaccgccacc
     4381 atctgctgct cgccgccgga aagataatcc gcaggactat ccagccgttc gcgaatccgc
     4441 gggaaatatt cgtagatccg ttgcctggtc cagtgcacgc cattgccggt ctggcgcttc
     4501 agcgcgccga gctcgagatt ctgttcgacg ctcatccctg cgaacaggcc gcggccctgc
     4561 ggcacataac caatgccgag ccgcgcggtc tgcgccgacg aaagtccgat caactcgctg
     4621 tccgcaagcc tgatcgatcc gttcgacgcc ggcgcgatcc cgatcagggt ctttaataac
     4681 gtcgacttgc cggcgccgtt gcggccgagc agcgcgatga tctcgttttc atgcaggctg
     4741 aagttcacat tgttgagaat gtggctcttg ccgtagaagg tatcgacatg ggttgccgtc
     4801 aatagcgcgt tgacgcgggc ggcggtttcg cgcggacgcg cggcaacctc ggtcgcgccg
     4861 gagccgatgt aaacttcctg caccttcggg ctggcgcgcg cgtcctccac cgaaccgtcg
     4921 agcagcaccc ggccttcatt catcacggtg acacgatcgg cgagctgaaa cacccggtcg
     4981 atgtcgtgtt cgaccagcag caccggcaaa tcggacgaga tgcgcttgat gatggcgccg
     5041 atgcgctcgc gctccgcggc ggcaagcccg gcgagcggct cgtccagcaa cagaatccgc
     5101 ggcgcggtgg caagcgccac gcccatgtcg agcaggcgct ggccgccata cgacaacatg
     5161 cccgcttcgg ccttttcgat gccggcaagg ccgagatagc ggatggtcgc atcggtctcg
     5221 gcattgatcg catcgatcga cagcgcattg gtgaggggat cgaagcggcg gggatgccgc
     5281 gcctgcacgg cgaggcggat gttttcggc
//
LOCUS       DATNOI010000163        14287 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1152528,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000163 DATNOI010000000
VERSION     DATNOI010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 14287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14287
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152528"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(386..898)
                     /gene="lepB"
                     /locus_tag="VN968_06175"
                     /pseudo
     CDS             complement(386..898)
                     /gene="lepB"
                     /locus_tag="VN968_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271942.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
     gene            complement(983..1825)
                     /locus_tag="VN968_06180"
     CDS             complement(983..1825)
                     /locus_tag="VN968_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL28860.1"
                     /translation="MNWDDLRIVAAVRDEGTYAGAGTRLRIDETTVGRRLARIQRELG
                     LRLFEAVDGLRKPTRQCEMVLAHVQAMAAHVAEINRVGESLPGLTGRFRIACTNSVAE
                     ELLSPRAGGFLLQNPGLTVQFLTASGNVKFSRWAADIAIRLRKPEKGDFTISKLADIR
                     LYFFEPAADPELEPVVCGYPEDLDSIPESQFLKARGLHPRSRCITDNVQVIKALIRTH
                     QAVGILPEYICADVLADRRLRATLLPRRRDVWLLVQNHLKRDPAARVVIDWVRDCFQE
                     ISKA"
     gene            1961..2590
                     /locus_tag="VN968_06185"
     CDS             1961..2590
                     /locus_tag="VN968_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HXL28861.1"
                     /translation="MLQLYFFPMACSLASRIALMEAGIDARYHLVHLWNKKVLEGDGD
                     FWAVSPKGAVPVLVLENGERLTENAAVLQYIADLRPELGLAPAAGDHDRYRLQEWLSF
                     VGTEIHKAFLFPTFWYANEAAKASARERIGKNLSVAAEHLESRQYVVGERFTVADAYL
                     AWSLLLLKRGGVDVNEWPSLVGYLNHIRERPQVKAAIDAEQEIRKTLAM"
     gene            complement(2629..3720)
                     /locus_tag="VN968_06190"
     CDS             complement(2629..3720)
                     /locus_tag="VN968_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tlde1 domain-containing protein"
                     /protein_id="HXL28862.1"
                     /translation="MSYRTRAPNNSAARNHRNSSGKTNPYITFGGAAVGCLVLGCAWI
                     VYANVFGASVYPTGGNFDVALIKRAPAPAVPNPPPVFDEVFALTSPAPLISTRVGLRL
                     GSSLTFDDRFAAASPQGEAPKPVPPPDRQVAAIAPPQADRQVAQLVRLPAPRPADAGA
                     ARNSTASARDMAQGAKAAVLAAVEEKPTIFEKLFGKLKSAGPSLAFASADASVTGSLG
                     SGNPGQSPNSALGGSVPYDRTTAVYDISAHVVYMPDGTKLEAHSGLGSKLDDPRFVNV
                     RMLGPTPPHVYDLQPRETLFHGVPALRLNPVGGDEAIFGRTGLLAHTYMLGPNGDSNG
                     CVSFKDYNAFLQAYRNGEVKRLAVVTRVQ"
     gene            complement(4003..4716)
                     /locus_tag="VN968_06195"
     CDS             complement(4003..4716)
                     /locus_tag="VN968_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008531683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HXL28863.1"
                     /translation="MSSSTLKPAAELARASGVRFPNESAEYRRAREALLAEEIELRRH
                     IERVAEQRRALPPGGEVKKNYAFEGEKGPVSFADLFGDKDTLVVYNYMFGPQRERPCP
                     MCTSLLSAWDGEVPDIQQRVALAVVARSPIERLKAFKRERGWHFLPLFSDISGEFSRD
                     YHALTKDGGDDAAFNVFTQQREAKGATIRHFWGGEMNGGSADPGQDPRGAPDLMPIWT
                     ILDVTPQGRGTNWYPKLNY"
     gene            complement(5090..6589)
                     /locus_tag="VN968_06200"
     CDS             complement(5090..6589)
                     /locus_tag="VN968_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXL28864.1"
                     /translation="MSPNEARLVRNTALAILALVALRLAAAAWTPLTFDEAYYWMWSK
                     HLAGGYYDHPPGVALVIRLGTMIAGDTELGVRLVSILLALPMSWAVYRAASILFGGIE
                     VAATAAILLNVTLMAAVGTLIVTPDSPLLVAASFVLFFLAKVLETGRGAWWLAVGAAV
                     GAALLSKYTSLFFGPAILIWLVAVPKLRRWLIAPWPYLGGLVSLLIFSPVILWNADHQ
                     WVSFIKQIGRARIEDFRPAFIGELIPTQIAFATPLVFILGAMGLYALARKAGALAARV
                     LINATFWTIVVYFIWHSLHARVEANWFAPVYPAFAIAAAVAAHLARWDLRPQRTVDFC
                     RRWAAPSGVLMFALLIVQANTGVLSAYRRDATVRSVGVGWGDLAGEIEAVRVRFGATC
                     VLAPDYGTTGWLAFYLPKGTCVVQPTQRIRWVNMPEPDPAQLAGKLLYVDEARPGGRP
                     YLNEMFARVERVAEFTRKRGPLAIETYALDLLENPKGDVLDRSSPPELE"
     gene            complement(6672..7805)
                     /locus_tag="VN968_06205"
     CDS             complement(6672..7805)
                     /locus_tag="VN968_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXL28865.1"
                     /translation="MNEAVRPGPENSSQTAGLPQLSVIVPTFNERDNVTTLLRRLETA
                     LTGVAWEVIFVDDNSPDGTWDVVRNLARGDPRVRCIRRINRRGLSGACIEGILASSAP
                     CAAIIDADLQHDETQLPVMLAPLQRGEADLVVGSRYVEGGSADSFNKQRAGASALATE
                     VAKRVLRVKIADPMSGFFMIRRDRFEQLAPELSTQGFKILLDIVATARGDLRIKEVPY
                     AFGSRLHGESKLDSMVALDFLGLVLAKLTHDVVSLRFLLFAMVGSIGLFVHLGTLFVT
                     LRLFDVPFPQAQAAGALVAMTSNFILNNFLTYRDQRLKGFAILRGLLLFYLVCSVGLL
                     ANVGVAFSVYDQEPIWWLAGAAGALMGVVWNYAMSGLFVWRKR"
     gene            complement(8109..8480)
                     /locus_tag="VN968_06210"
     CDS             complement(8109..8480)
                     /locus_tag="VN968_06210"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL28866.1"
                     /translation="MPSLAGLHTPSEDQWFFRERVFETCEVWGAFDSAGMTGMIAFQK
                     DWIDQLYVQPDAQRRGVGTELLQVAKSSFDRLQLWTFQCNVRARRFYEGKGFARVSGP
                     ADRPAVGDPCRNGSACLIDEV"
     gene            9133..9474
                     /locus_tag="VN968_06215"
     CDS             9133..9474
                     /locus_tag="VN968_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HXL28867.1"
                     /translation="MQSEKLEPCQDGSKQADPAVAPENAVPVDFQTLATAYGNYTKKS
                     FEQTQAFVEKLSGVRSLDKAVEIQTEFAKQAYETFVTESQKIRELYRGLAKRNLRPFE
                     GLVAKKTPTSH"
     gene            complement(9644..10084)
                     /locus_tag="VN968_06220"
     CDS             complement(9644..10084)
                     /locus_tag="VN968_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28868.1"
                     /translation="MIGQFAQTVRPRLPRPGVQARIRKKGNEMRMIMAAALAMTTSVA
                     IADDVEDAHRQAISGRDSYWNCLAQEYTRDGNKSMSEQDFTVHIASVCPSERQNFRVT
                     LLDYLTMQFPGAEAGAHMTTANNAIAFAQKDIVTAFARHKAPPK"
     gene            10146..10970
                     /locus_tag="VN968_06225"
     CDS             10146..10970
                     /locus_tag="VN968_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXL28869.1"
                     /translation="MSKPPQTPSKHSGHIYIGIGGWTFAPWRGVFYPEKLTQAKELEY
                     AASKLTSIEINGTYYGSQKPESFRKWAREVPDGFVFSLKGPRFATNRRVLAEAGDSIK
                     RFYDSGVLELGDRLGPVLWQFAPTKKFDEADFGKFLELLPRTLEGRALRHVVEVRHDS
                     FCVPDFIALLRQFETPVVFAEHGKYPAIADVVGDFVYARLQKGNDELKTCYPPKALDA
                     WAKRFQLWAGGGEPDDLPRVDAAKPKKAPRDVFAYVIHEGKVRAPAGAMELIERVK"
     gene            10984..11445
                     /locus_tag="VN968_06230"
     CDS             10984..11445
                     /locus_tag="VN968_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HXL28870.1"
                     /translation="MAKAKKLFTIGYEQTPAKAVLDELEQAGVKLLVDVRAVAASRRP
                     GFSKNQLAAGLDERGISYLHLRGLGTPKDGRDAARSGKFDALHKIYAKHLKAPQAKEE
                     LDELSALVKKSGPVCILCYERDHLHCHRQWIAEIIEDRDGVKIENLVAPQA"
     gene            complement(11634..12545)
                     /locus_tag="VN968_06235"
     CDS             complement(11634..12545)
                     /locus_tag="VN968_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HXL28871.1"
                     /translation="MKRRYITVDVFTDRAFGGNPLAVVLDSGGLSIAQMQSIASEFNY
                     SETTFVLPPQDPTHDAQVRIFTVKYEIPFAGHPNVGTAFVLATQTPKPPARLKFEEKA
                     GLVPVEILTEEGRVIGAELTAPQALKKLTPFSAEQAAACISLTAADVRTDRHPPQIVS
                     VGLPFLVVEVASRDALRRARPVASAFARTFPCDGSDAVYFYTRDVPPAEACDLQARMF
                     HPGSSGLSEDPATGSATVAAAALLADLASERDGELKLRIGQGVDMNRPSLLLTRVVKQ
                     NGAIVSAHVGGACVQMMEGTFRLAGEG"
     gene            complement(12620..13933)
                     /locus_tag="VN968_06240"
     CDS             complement(12620..13933)
                     /locus_tag="VN968_06240"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL28872.1"
                     /translation="MAEFWRGIKGERRPAVPADFQHALMQEVLRSELIRIKALIATAA
                     LLAVVLWTVYLLDPDAINHVWRGRLRPAYLYGILVPFILFELWVHAMISRHLRLDRDV
                     PVIRRYLGALIETTMPTAALALHIENMGAVQALGFVAPLAYFLFIILSTLRLDFWLST
                     FTGFVAAAELFAMALFYHPDGGADLQPEIFYHAARSLIVLVGGVLAGAVGVQVRRQFE
                     ASIQAATARDRITNLFGQHVSPEVVERLMVEGTNSASDIRRVAVMFVDFRSFTAGARS
                     RSPQDVVERLDGAFAVLVDILDRHGGIVNKFLGDGFLALFGAPFENADAAHHAVDAAR
                     KMIGAMETINAASSWPLRIGIGIHFGEVVAGNIGSPRRKEYTVIGDTVNFAARLEALN
                     KEFNSQLLISAAVRDAIGNDSDDAVPLGEVAIRGYDRPMTVWQLG"
     gene            complement(14101..>14287)
                     /locus_tag="VN968_06245"
     CDS             complement(14101..>14287)
                     /locus_tag="VN968_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL28873.1"
                     /translation="MPVWAQYFGEGLPLTHYLRIVRAIMLKGATLPNLQYDTIALLAL
                     MLLAMTIAVTRFRRTLD"
BASE COUNT         2936 a         4488 c         4384 g         2479 t
ORIGIN      
        1 agccggcggc gaacagttcg ggccaatcct cggccaccac aggccgtatg ataacggtcg
       61 gtccgatcag ggttggttga aaatcaggtt ttgacatgcc tagctgatag cgttgttcgg
      121 acttggggcg caagcggcca gtgccccgtt tcgatggaat cgaaacgagg ctgattgacg
      181 cgctttctta gcgcgaaccg gtcgccgccc cggatcaagt ccggggcagg ctttcgcttg
      241 aaaacgctct gccggaaagt gaagggccgc ccgtgggcgg ctcatgccgt ttccgttggc
      301 ttcaggacca gcttgttgag cagcgcatca aagccctgcc cgtcgcctgc gtgaaggtcg
      361 atccgtccga ccttgcccga accccctcat gactgacgcc gttcggcagg gtttcgcgcc
      421 agtgcttcac cctggcggcg ggatcggacc cgcacagatc accgccgatg aagtccacca
      481 accgctcgcg ctgcaccggc gtgccgttga tggtgagcag cccctgtttc atttgaatac
      541 gatcgccgag caatcccacc acacgcttga cgtaatcggt cgaagtattt ttcggcaaac
      601 gaaatacaac gacgtcgccg cgcgcgggtt cggagccgaa gatgcgtccg gaaaacagcg
      661 gcggcgaaaa tggcagcgaa aaatggctgt agccataagc acatttcgag acaaagaaag
      721 aatcgccctg ctgcaaggtc gggctcatcg aaccgcttgg ggcactgaag ggctggaaca
      781 ggaacgtacg aacaaatatg gggctcgata ccaataagat cagcagaatc acgccgacga
      841 cgccaagcca taccctattc cacggcgatc gcgggcttgc cgcttgagta aacgtcatga
      901 atgacccggg cctggagtgg ttcagccaat ttaaccatca tggagtgaag cgcaacacgt
      961 ttggtcatcg cgcgctacaa agctacgcct tcgaaatttc ctgaaagcaa tcgcgaaccc
     1021 agtcgatcac gacgcgcgcg gcgggatcgc gcttgaggtg gttctgcacc aacagccaca
     1081 cgtcgcggcg ccgcggcagc agcgttgccc gcaggcggcg gtcggccagc acatctgcgc
     1141 agatatattc aggcaggata ccgacggcct gatgggtgcg gatcagcgcc ttaatgacct
     1201 gaacattgtc ggtgatacag cgcgagcgcg gatgcaatcc cctcgccttc agaaactgcg
     1261 actccggaat ggaatcgagg tcctcgggat aaccgcagac caccggctca agttcggggt
     1321 cggccgccgg ctcgaagaag tacagcctga tatcggccag cttcgagatc gtgaaatcgc
     1381 ctttttccgg cttacgcagg cgaatcgcga tatctgccgc ccatcgcgag aatttcacgt
     1441 tgccgctggc ggtaaggaat tgaacggtca ggcccggatt ctgcaaaagg aacccgccag
     1501 cgcgcggaga caacagctcc tcggcgacag aattggtgca ggcaatccgg aaacgtccgg
     1561 tcaaccctgg caggctttcg ccaacccggt tgatctcggc cacatgcgcc gccatcgcct
     1621 ggacatgcgc gagcaccatc tcacactgcc gggttggttt gcgtaacccg tcgacggctt
     1681 cgaacaaccg caggccgagc tcgcgctgga tccgcgccag acgccgcccc accgtcgtct
     1741 catcgatgcg cagacgggtg ccggccccgg cataggtgcc ctcgtccctc accgcggcaa
     1801 cgatgcgaag gtcgtcccag ttcatgggcc ctgctaccac ggacctcgca ttacctgcaa
     1861 tatcgcagcc acaggctgcg atatccctgc gccagagcag ggcgatcttc gcgctatatg
     1921 ggcccgtggc aattctgttg ggcgatgacg gagacgaaca atgctgcagc tctatttctt
     1981 cccaatggcc tgttcgcttg ccagccggat cgcgctgatg gaggccggca tcgatgcgcg
     2041 ctatcatctg gtgcatctct ggaacaagaa ggttctggaa ggcgacggcg atttctgggc
     2101 ggtttcgccg aagggggccg tgcccgtgct ggtgcttgaa aacggcgaac ggctgaccga
     2161 aaacgcggcc gtcctgcaat atattgccga cctgcggccg gaactaggcc ttgcgccggc
     2221 ggccggcgat cacgaccgct accgcctgca ggagtggctc tcgttcgtcg gcaccgagat
     2281 ccacaaggcc tttctgtttc caaccttctg gtatgcgaac gaagcggcca aggcgagcgc
     2341 gcgagagcgc atcggaaaga acctgtcagt tgccgccgag caccttgaaa gccggcaata
     2401 cgtcgtcggc gagcgcttca ccgtggcgga tgcgtatctg gcgtggtcgc tgctgctgct
     2461 caagcgtggc ggcgtcgatg tgaacgaatg gccgtcgctg gtcggctatc tcaaccacat
     2521 ccgggagcgc ccgcaggtca aggcggcgat cgacgcagag caggagatcc gcaagacgct
     2581 ggcgatgtga cggtacgggc gacgacgtcc cggatcgcca ccagccgctc actgcacgcg
     2641 cgtgaccacg gccagccgtt tgacctcgcc gttccggtag gcctgcagga acgcgttgta
     2701 gtccttgaac gaaacgcagc cgttggaatc gccgttggga ccgagcatgt aggtgtgcgc
     2761 gagcaggccg gtgcgtccga agatcgcttc gtcaccgccg acgggattga gccgcagcgc
     2821 tggcacgcca tgaaacagcg tctcgcgcgg ctgcaggtca tagacatgcg gcggcgtcgg
     2881 acccagcatt ctgacgttga caaaacgggg gtcgtcgagc ttggagccga gaccggaatg
     2941 cgcttccagc ttggtgccgt cgggcatgta aaccacgtgc gcggagatat cgtaaaccgc
     3001 ggtcgtgcga tcatacggca ccgatccgcc cagcgcgctg ttcgggctct gaccggggtt
     3061 tcccgatcca aggctgccgg tcacgctggc atccgcggag gcgaaggcca gcgacggtcc
     3121 ggccgacttc aacttgccaa acagcttttc gaagatcgtg ggtttttctt cgaccgccgc
     3181 cagcacggcc gccttggcgc cctgcgccat gtcccgggcg gaagctgtgg aatttctagc
     3241 cgctccggca tcggcggggc gcggcgccgg cagccgcacc agctgagcga cctggcgatc
     3301 agcttgcggc ggcgcgattg cggcgacctg gcgatccggc ggcggcaccg gcttcggcgc
     3361 ttcgccctgt ggcgatgccg cggcaaaccg gtcatcaaac gtaagcgatg agcccaaccg
     3421 cagaccgacc cgcgtggaaa tcagcggtgc gggtgaggtc agagcgaaga cctcatcgaa
     3481 aaccggtggc gggttcggaa cggccggcgc tggcgcccgc ttgatcagtg cgacatcaaa
     3541 gttgccgccg gtgggataga cgctggctcc gaacacattg gcatacacga tccaggcgca
     3601 gcccagcacg aggcacccga cggccgcgcc gccgaacgta atataggggt tggttttccc
     3661 ggaggaattt ctgtggttgc gtgccgcgga attgttaggt gcgcgggtgc ggtaactcat
     3721 tcgccttgca tccagactag ctccaccttg ttccccttcc aggcagcctt cgagcgtgtg
     3781 cgtttcaccg gcacgttcga aatgcaaatc tgcgcgcaat gccccgaaga atcagaagtc
     3841 ccaaggatgc atctcgcaga ggcacggagc gtcattaagg cggtttttag ttaaatgcgg
     3901 attaagtgtg ggcggatgtt gggcaggtgg aaacacggag atcgccccgg cgggcggcga
     3961 tctccgtgtt tggcccgatt tagagcgcaa aaacgggtgt tttcagtaat tcagcttcgg
     4021 ataccagttc gtgccccgcc cctgcggcgt gacatctaaa atcgtccaga tcggcatcag
     4081 atccggcgca ccgcgcggat cctgtccggg atcggccgac ccgccattca tttcgccgcc
     4141 ccaaaaatgc cttattgtgg cacctttggc ctcgcgctgt tgcgtgaaaa cgttgaacgc
     4201 cgcgtcgtcg ccgccgtcct tggtcagcgc atgatagtct cggctgaatt cgcctgagat
     4261 gtccgagaac agcggcagaa aatgccagcc gcgctcgcgc ttgaaggcct taagccgctc
     4321 gatcggcgag cgcgcaacca ccgccagcgc gacgcgctgt tggatgtccg gaacctcgcc
     4381 gtcccaagcc gatagtagcg acgtgcacat cgggcacggc cgctcgcgct gcgggccgaa
     4441 catgtaattg tagacaacga gggtatcctt gtcgccgaac aggtcagcga aggagacggg
     4501 tcccttttcg ccctcgaagg cgtaattctt cttgacctcg ccgcccggcg gcaacgcgcg
     4561 gcgctgctcg gccacgcgct cgatgtgacg gcgcagctcg atctcttcag ccagcagcgc
     4621 ctcgcgggcg cggcgatatt cggcgctctc gttgggaaaa cgaacaccac tggcgcgcgc
     4681 cagctccgcg gccggtttca gagtagaact gctcatggat ttcagcgtgg aactggtcat
     4741 ggcatgcctc gcttcaatgg attttggggt cccgaagata agacgtttcg gcagcggacg
     4801 atccgacagg tggcagcgct aattttcggc cgccatgcgc gggctgaccc gtgtatccat
     4861 cgatcttcgg aggagtcttg cgaagatgat ggattgccgg gtcaagcccg gcaacgacga
     4921 aagtgtacgg caaggccgag ctcgtgtcta ggacgcagtg cagcgtcctt cacgctgcgc
     4981 cgcagagccg ggacccaccc cgccgcaaac gatgggcccc ggatcagcag cgcaccgcaa
     5041 gcgcgctgcg cagcatccgg ggcacgtgac cgcgtaccga gccggcccct tattccagtt
     5101 ccggcggcga cgaacgatcc agcacgtcgc ccttgggatt ttcgagcaga tcgagcgcgt
     5161 aggtctcgat cgcgagcggc ccgcgcttgc gcgtgaactc cgccaccctt tcgacccgcg
     5221 cgaacatttc gttcagatac ggccgcccgc ccggccgcgc ctcatcgaca tacaacagct
     5281 tgccagccaa ttgcgccgga tccggctcgg gcatgttgac ccagcggatc cgctgcgtcg
     5341 gctgcaccac gcaggtgcct ttcggcaaat agaaggcgag ccagccggtg gtgccgtaat
     5401 ccggcgccag cacgcaagtg gcgccaaagc ggacacgaac cgcctcgatc tcgcctgcaa
     5461 ggtcgcccca gccgacgccg acgctgcgca cggtggcgtc gcggcgatag gccgacagca
     5521 cgccggtatt ggcctgcacg atcaacagcg caaacatcag gacgcccgat ggcgccgccc
     5581 agcgccggca aaaatccacg gtgcgttgcg gccgcaggtc ccatcgcgcg aggtgggcgg
     5641 cgacggcggc tgcgatcgcg aacgccggat agaccggcgc gaaccagttg gcctcgacgc
     5701 gggcgtgcag cgaatgccag atgaaataca cgacgatggt ccagaacgtc gcattgatca
     5761 gcacgcgcgc cgccagcgcg ccggccttgc gcgccagcgc gtaaagtccc atcgcgccga
     5821 ggatgaacac cagcggcgtt gcgaaggcga tttgcgtcgg gatcaactca ccgataaacg
     5881 ccggccggaa atcctcgatt ctggcgcggc cgatctgctt gatgaaggag acccactggt
     5941 gatcggcatt ccacaggatg accggcgaaa atatcagaag cgaaacgagg ccgccgagat
     6001 atggccaggg cgcgatcagc cagcgccgca atttcgggac agccaccagc cagatcaaga
     6061 tcgccgggcc gaaaaacaat gaggtgtatt tcgacagtaa cgcagcacca acggcggcgc
     6121 cgaccgcgag ccaccacgca ccgcgccccg tctccagtac tttggcaaga aaaaacagca
     6181 caaagcttgc ggccaccagc aacggtgaat ccggcgtgac aatgagggtg ccgaccgccg
     6241 ccatcagcgt gacgttcagg aggatcgccg cggtcgccgc cacctcgatg ccgccgaaca
     6301 ggatcgaggc tgcgcgatac accgcccagc tcatcggcag cgccagcaat atcgacacca
     6361 gcctcacgcc aagctcggtg tcgccggcaa tcatggtgcc gagccggatc accagcgcga
     6421 cgcccggcgg atgatcgtaa tagccgccgg cgagatgttt cgaccacatc cagtaatagg
     6481 cttcgtcgaa ggtcagcggc gtccaggccg cggcggccag ccgcagcgcc accagcgcca
     6541 aaatcgcaag agctgtgttg cggacgagcc gcgcctcgtt cgggctcatg gtcgactcac
     6601 ggcctgcggt ccaggacttg cggatccaag acttgctggt ccaagacttg tggattcaaa
     6661 acttggcagg ttcatcgctt gcgccagacg aacagtccgg acatcgcgta attccatacc
     6721 acgcccatca gcgcgccggc cgcgccggcc agccaccaga tcggctcctg atcgtaaacc
     6781 gagaacgcca cgccgacatt ggcgagcagg ccgacgctgc acacgaggta gaacagcaag
     6841 aggccgcgca ggatcgcgaa acccttcagc cgctggtcgc gataggtgag aaaattgttc
     6901 aggatgaaat tgctggtcat cgcgaccagc gcaccggcgg cctgcgcttg cggaaacggc
     6961 acatcgaata atcgaagcgt gacgaacagc gtgccgagat gcacgaacag gccgatcgag
     7021 ccgaccatcg caaacagcag gaagcgcagc gacaccacgt cgtgggtcag cttcgccagc
     7081 accaggccca agaaatccag cgccaccatg gaatcaagct tgctctcgcc atgcagccgc
     7141 gagccgaagg catagggaac ttccttgatg cgaagatcgc ctctggcggt cgcgacaata
     7201 tccagcagga tcttgaaacc ttgcgtcgag agctccgggg ccagttgctc gaagcgatcc
     7261 cgccggatca tgaagaatcc gctcatggga tcggcgatct tgacccgcag cacgcgcttg
     7321 gcgacttcgg tggcgagcgc actggcccct gcccgctgct tgttgaaact gtcggcgctg
     7381 ccgccttcga catagcggct gccgaccacg agatcggcct caccacgctg cagcggggcc
     7441 agcatgaccg gcagctgggt ctcgtcgtgc tgcaggtcgg catcgatgat cgcagcgcac
     7501 ggcgcgctcg atgccaaaat gccttcgata caggccccgg acaggccgcg ccggttgatc
     7561 cgccggatac agcggacgcg gggatcgccg cgcgccagat ttcgcaccac atcccaggtg
     7621 ccgtcagggg aattatcgtc gacgaagatg acttcccagg cgacgccggt cagcgcggtc
     7681 tccagccgcc gcaacagcgt ggtgacgttg tcgcgctcgt taaaggtggg gacgatgacc
     7741 gaaagctgcg gcaaaccggc ggtctgcgac gaattttcgg ggcccggtct gacggcttca
     7801 ttcatgccgc cagcctatat ccgcagcctc aggggaagcc aaggcgcagt tccgaacccg
     7861 atgggcaaga tgttgatgca ggggagcgaa acggcccaaa aatgccaacg accacgaaca
     7921 gagggcgcgc gtacatccgg cgcagccgag ctattcagtg gtcgtcccag cgccggagct
     7981 atgaagcatc gacgtcgccg ttggatacaa catagagatg atttgggtgt ttcgcaagag
     8041 ccctgaatgt ccgaggaccc ggttcaatgg aactttaatc agcctgcgcc cctgtgcgag
     8101 gaccaatttt agacctcgtc tatcaaacac gcgctaccgt ttctgcaggg atcgccgacc
     8161 gccggcctgt cagcgggtcc agagacccgc gcaaagcctt tcccttcgta aaagcgccgg
     8221 gcccgcacat tgcactggaa cgtccaaagc tgcaggcgat cgaacgaact cttggcgact
     8281 tgcagcaact cggtgccgac gccacgccgt tgcgcgtcgg gctgcacgta caattggtcg
     8341 atccagtcct tttggaaggc gatcattcca gtcatcccgg cgctgtcgaa agcaccccag
     8401 acctcgcagg tttcaaacac gcgctcccga aagaaccatt ggtcttcact gggcgtatgg
     8461 agaccggcga gcgaaggcaa ggcgtgatcg gctctaacga cccgcgcggc ggtgtccatg
     8521 tcttcaagcc tgatctgcct taacatccgg tggataggtc cgctgtctca attacttaat
     8581 gcaagaaatt gtggaatatg gctcagccga actattccct taatgctatt cctgaagcgt
     8641 cgtacaaacc cctatttggc aacacacatc aggcggcaag gcgcagatac tcagtgaaat
     8701 cgggacaggg ctcagtgcag cggccgcggt tccgaattct tgatcccaac acaccagcgc
     8761 cgcgcaagga acaagccata gccgcgattt cttcgcacct acggccgact ctcacgctat
     8821 ggtagtaaaa ctatggagat tcgcaaaatg tcgggcccaa atgagaacaa gccgggcaag
     8881 aagaagcacg gcaaggggag ccgcaaggcg aaaccgtcgc tgaatcagaa accggatcaa
     8941 atgctcgacg agaagccgga gccggtgctg agccacaagc tggagcggct ggcgagccac
     9001 gagccggagc cactgcagag ctacgagccg gagccactgg cgagccgcga gccggagcca
     9061 ctgcagagcc acgagccgga gcaactgctg agccccgagc cggaaccgct gcagatccac
     9121 gagccggagc aactgcagag cgagaagctg gaaccatgtc aggacggcag caagcaggcc
     9181 gacccagccg ttgcgccaga gaacgccgtc ccggtcgact ttcagacgct cgcgacggcg
     9241 tatggcaact acaccaagaa gtcttttgaa cagacccaag cctttgtcga gaagctatcg
     9301 ggcgtgcggt cgctcgacaa ggcggttgag atccagaccg agttcgccaa gcaggcttat
     9361 gagactttcg ttaccgagtc gcagaagatt cgcgagctct accgcggcct tgccaagcgg
     9421 aatctcaggc ctttcgaggg cttggttgcc aagaagaccc cgacatcgca ctgaaatctt
     9481 gcggcacttc gtcgcctcat gggccccttg gtgccggctg cgcaaagagc agaggctccg
     9541 gtttgtggct gcttgtttcg cctacccgaa ccaatccccg tcgatcccgc cgctaaggta
     9601 aggtcccgac aaacgaggca aatacaggca acgctcgcgc cggctacttc ggcggagctt
     9661 tgtgcctggc gaatgccgtc acgatatctt tttgagcgaa agcgatggcg ttgttggcag
     9721 tcgtcatatg ggcacccgcc tcggcacccg gaaattgcat cgtcagatag tccaacagcg
     9781 taacccggaa attctgccgc tcggaaggac acacgctcgc gatatggacg gtgaaatcct
     9841 gttcggacat gctcttgttg ccgtcccgcg tatactcctg agcgaggcaa ttccagtatg
     9901 aatcacgtcc agatatggct tgccgatgcg catcttcgac gtcgtcggcg attgccactg
     9961 aagtggtcat cgccaacgct gccgccatta tcatccgcat ctcgttcccc tttttcctga
    10021 tcctagcttg aacgcctggc cgaggtaagc gcggacgcac ggtttgagcg aactggccaa
    10081 tcacgattga tttgattagt ctcgatccga catatcaagt ctgccctttg caatgagacc
    10141 cgccggtgag caagcccccg caaaccccgt caaaacattc aggccacatc tacatcggca
    10201 tcggcggctg gaccttcgcg ccgtggcgcg gggtgtttta tccggagaag ctgacgcagg
    10261 cgaaggagct ggaatacgcg gcctccaagc tgacctcgat cgagatcaac ggcacctatt
    10321 atggttcgca gaagccggaa agctttcgca aatgggcgcg cgaggtgccg gacggttttg
    10381 tgttctcgct gaaggggccg cgcttcgcca ccaatcgccg cgtgctggcg gaggccggcg
    10441 attccatcaa acggttttac gattccggcg tgctggaact gggcgaccgg ctcggcccgg
    10501 tattgtggca attcgcgccg accaagaaat tcgacgaggc ggattttggc aagtttctcg
    10561 agctgctgcc gcgcacgctt gagggccgcg cgctgcgcca tgtggtcgag gtgcgccacg
    10621 acagtttctg cgtgcctgac ttcattgcgc tgttgcgcca attcgagacg ccggtggtgt
    10681 tcgccgagca tggcaagtat ccagcgatcg cggacgtcgt cggcgatttc gtctatgcgc
    10741 ggctgcagaa gggcaacgac gagctaaaaa cctgctatcc gccgaaggcg ctcgacgcct
    10801 gggccaagcg gtttcaactc tgggccggcg gcggcgagcc cgacgacctg ccgcgcgtcg
    10861 atgccgcaaa gccgaaaaaa gcgccgcgcg atgttttcgc ctatgtgatc cacgaaggca
    10921 aggtacgcgc acccgccggc gcgatggaat tgatcgagcg cgtcaaataa agcggggcgg
    10981 caaatggcaa aagccaaaaa gctctttacc atcggctatg aacagacgcc cgccaaggcg
    11041 gtgctcgacg aactcgagca ggccggcgtc aagctgctgg tcgatgtacg tgcggtcgca
    11101 gcttcccgac ggcccggctt ttcgaagaac cagcttgcgg cagggctcga tgagcgcggc
    11161 atttcatatc tgcatctgcg cgggctcggc accccgaagg acggccgcga cgctgcgcgc
    11221 agcggcaagt tcgacgcgtt acacaagatc tatgcaaaac atctgaaggc gccgcaggcc
    11281 aaagaagaac tcgacgaatt gtcggcgctg gtgaagaaat ccggaccggt ctgcatcctc
    11341 tgctacgagc gcgatcatct gcactgccac cgccagtgga tcgccgagat catcgaagac
    11401 cgcgacgggg tgaagatcga aaatctggtc gcgccgcagg cgtagcgatt ttcaggttgt
    11461 ctgacgtgcc cggcgacaat aaaggctgtc attccggcgc gcgcgaagcg cgagcccgga
    11521 atcgataacc acgatcggga gtatgggttc cgggcctgtc gcctcaggtc ggcaattgcc
    11581 gaccgaagac gcatcccgga atgacgatct caatctggaa acgctgcgcc ctctcacccc
    11641 tccccggcca gccgaaacgt cccttccatc atctgcacgc aagccccgcc gacatgcgcg
    11701 gaaacgatcg cgccgttctg cttcacaaca cgcgtcagca gcaggcttgg ccggttcatg
    11761 tcgacgccct gcccgatgcg cagcttcaac tcgccatcgc gttcgcttgc gagatcggcg
    11821 agcagtgccg cggctgccac ggtggcgctg ccggtggcgg gatcttcgga caggccgctc
    11881 gatcccgggt gaaacatccg cgcctgcaaa tcgcaggcct cggccggagg cacgtcgcgg
    11941 gtgtagaaat agaccgcatc gctgccgtcg cacggaaatg tgcgggcgaa tgccgatgcg
    12001 accggcctgg cccgacgcag cgcgtcgcgt gaggccacct cgaccaccag aaacggcagc
    12061 ccgaccgata cgatttgcgg cggatgccgg tcggtcctga cgtccgcagc cgtcagcgag
    12121 atacaggccg cggcctgctc ggcgctgaat ggcgtgagct tcttgagggc ctgcggcgcg
    12181 gtgagttcgg cgccgatgac ccttccctcc tcagtcagga tctcgaccgg caccaggcct
    12241 gctttttcct cgaactttag ccgcgccggc ggctttggcg tctgcgtcgc cagcacgaag
    12301 gcggtgccga cattgggatg gcccgcgaac gggatttcgt acttcacggt gaaaatgcga
    12361 acctgcgcat cgtgcgtcgg atcctgcggc ggcaacacaa aggtcgtttc ggaataattg
    12421 aactcggacg cgattgattg catctgcgcg atcgagagcc caccggagtc gagcaccacg
    12481 gcaagcggat tgccaccaaa ggcgcggtcg gtgaaaacgt ccacggtgat gtagcgccgt
    12541 ttcatctgag atctccgatt ttgtataagg tgaaacatga cgcggatcgg ttcgtcaagc
    12601 gtagcgagag tccgcgtttt tatccaagct gccacaccgt catcggccgg tcgtatcccc
    12661 tgatcgcgac ctccccgagc ggcacggcat cgtcgctgtc gttgccgatc gcgtcacgaa
    12721 cggcggccga aatcagcagc tgcgaattga actccttgtt gagcgcttca agccttgctg
    12781 caaaattcac ggtgtcgccg atcacggtgt attccttgcg ccggggagag ccgatattgc
    12841 ctgcgaccac ttcgccgaaa tgaatgccga tgccgatgcg cagcggccag ctgctcgccg
    12901 cgttgatggt ctccatcgcg ccgatcattt tccgcgcggc gtcgaccgcg tggtgcgcgg
    12961 cgtccgcatt ctcgaacggc gccccgaaca acgccagaaa gccgtcgccc aggaacttgt
    13021 tcacgatgcc gccatggcga tcaagtatgt ccaccagcac cgcgaacgcg ccgtcgaggc
    13081 gctcgacgac gtcctgcggc gagcgcgacc gcgcacccgc cgtgaaactg cggaaatcga
    13141 cgaacatcac ggcgacccgc ctgatatcgc tggccgaatt ggtgccctcg accatcagcc
    13201 gctccaccac ttccggcgaa acgtgctggc cgaacaggtt ggtgatgcga tcgcgcgcgg
    13261 tcgcggcctg aatgctggcc tcgaactggc gacgcacctg cacgccgacc gcccctgcca
    13321 gcaccccacc gaccaggaca atcaggctgc gcgccgcgtg ataaaatatc tcgggttgga
    13381 ggtctgcgcc gccatcggga tgatagaaca gcgccatggc gaacaattcc gccgccgcga
    13441 cgaagccggt aaaggtcgaa agccagaaat ccagccgcag cgtcgagaga atgataaaga
    13501 ggaaataggc gagcggcgcg acgaagccca gcgcctgaac cgctcccatg ttttcgatgt
    13561 gcagggccag cgctgccgtc ggcatcgtgg tttcgatcag cgctccaaga tatcgcctga
    13621 tcaccggcac gtcgcggtcc agccgcaggt gccggctgat catcgcgtgc acccaaagct
    13681 cgaacaagat gaacgggacc aggatcccat agaggtaggc tggcctcaga cgacctcgcc
    13741 agacgtgatt gatcgcatcg ggatcgagga ggtagacggt ccagaggacc acggcgagaa
    13801 gtgctgcggt tgcgatcagc gccttgatcc tgatcaactc gctgcgcagc acttcctgca
    13861 tcagcgcatg ctggaaatcg gccggcaccg ccggccgccg ctcaccttta atgccgcgcc
    13921 aaaattcagc catcacgtta tccagtcatt ccgggacacg cgcgagcgtg gacctcagat
    13981 gtgtgattgc acatcgggga atctcgagat tccgggttcg ctcgcctagc gcaaaattgg
    14041 cgcgaagcgc caattttgtc gccgggctcc cgcccgggaa atgacagtaa gcattctgca
    14101 tcaatcgagg gtgcggcgga agcgcgtcac cgcgatcgtc atcgctagca gcatcaatgc
    14161 cagcagggca atcgtatcgt attgcagatt tggcagggtc gcgcccttca gcatgatggc
    14221 gcggacgatc cgcaggtaat gcgtcagcgg caggccttcg ccgaaatatt gcgcccagac
    14281 cggcatg
//
LOCUS       DATNOI010000164         3892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1365733,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000164 DATNOI010000000
VERSION     DATNOI010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365733"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VN968_06250"
     CDS             <1..868
                     /locus_tag="VN968_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL28874.1"
                     /translation="QALVDALNSKHVAGAAFDVFVEEPATKNVLFGHPNVICTPHLGA
                     STTEAQENVALQVAEQMSDYLLTGAISNAVNFPSITAEEAPKLKPFIELAEKLGSFAG
                     QLTESGILKVQITYEGHVAEMKIKAITSAVLSGLLRPMLGDVNVVSAPIVAKERGMVV
                     DEIVRAAQSDYESLITVTVTTERQERSVSGTVYADGKPRLVDIKGIRVDAEFGKSMIY
                     VTNEDKPGFIGSFAGLLGAAQINIATFHLGRNKPGGDAIALVEVDGPVPAALLAKLQT
                     LPQVKQAKALVF"
     gene            complement(900..1676)
                     /locus_tag="VN968_06255"
     CDS             complement(900..1676)
                     /locus_tag="VN968_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28875.1"
                     /translation="MDSDAVFRAKLGGRLVMGASAGVIVLGLVVICASAWGQNSSVKD
                     AAQLIFTAVLPLLGTWVGTVLAFYYTRENYEAASRSTLEAVRSGSQQLASTRVTDKMM
                     PAAAIVKANVPPAKAIDDLLLKDIGDLFDKMLPNGQKISRLLVVNDKFACIAIIHRSI
                     WMEMLNTGAKQAPPVDPARDNLGKLLDKPYVSAVGKTFKDFITGTLAYIAIDRTLADA
                     KAAMEAKPQCQDVIVTETGKSDERMQGWISNVDISRLSQA"
     gene            1965..3632
                     /locus_tag="VN968_06260"
     CDS             1965..3632
                     /locus_tag="VN968_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HXL28876.1"
                     /translation="MPIVANRLVMTSLVVFTTLAAMQAKAANVEATSAVDAVTIYPDG
                     ASVTRAITVDVPSGDNTLVIKDFPLTLDPSSLRVEGEAGAKLTIGAIDTRPPRAAPPV
                     NLPELDQRIEALKDERANLQGAITAATARRKFAERFAETSPAGIGEKGEARPIADWRA
                     AFAAVAEEVASADAAIRDAERKQRDIDREIARLESDRAIKPPSKLEVRIDLSAAAATK
                     ATLRGTYAVRAARWTPLYDARLDTGAKDRKPALELVRRAEITQTTGEDWPNIALSVST
                     VRSARGGRAPELNSLIVQYPPPARPAAPAGAVMDNARPPAQSRAMSKALDQPAEREKA
                     DEQQAAAEVGGFQVVFRIPGRVSVGASEGAKSLRVSTASIAPDLAVRSVPVIDPTAFL
                     EASFTQNEDAPLLPGRVAIYRDGVFIGRSHVAAAGKDETVRLGFGADDKVKVERSVVK
                     RNEGSAGLILTTSKTDERSFKTVIRNGHDFPIRIAIEDQLPVSENEDIQVEMLPSTTP
                     PAVTNVRDRRGVLEWAFEAKAGEVRDIAFAWRVRWPKDKGVVMTPAG"
     gene            complement(3745..>3892)
                     /locus_tag="VN968_06265"
     CDS             complement(3745..>3892)
                     /locus_tag="VN968_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HXL28877.1"
                     /translation="IENVDRVDETHIIVGNDNNLPFSSSRDPNKADDNELVLLEVGDF
                     LKAK"
BASE COUNT          754 a         1180 c         1218 g          740 t
ORIGIN      
        1 gcaggcgctg gtcgacgcgc tgaattccaa gcatgtcgcg ggcgccgcct ttgacgtgtt
       61 cgtcgaggag cccgccacca agaacgtgct gttcggccat cccaacgtga tctgcacgcc
      121 gcatctcggc gcctccacca ccgaagcgca ggagaacgtc gcccttcagg tcgccgagca
      181 gatgtcggat tatctgctga ccggcgcgat ctcgaacgcg gtcaatttcc cttcgatcac
      241 ggccgaagaa gcgccgaagc tgaagccgtt cattgaactg gccgaaaagc tcggctcgtt
      301 cgcgggccag ctcaccgaga gcgggatcct caaggtccag atcacctatg agggccatgt
      361 cgccgaaatg aagatcaagg cgatcacctc ggcggtgctg tcgggcctgc tgcggccgat
      421 gctgggcgac gtcaacgtgg tctcggcgcc gatcgtcgcc aaggagcgcg gcatggtggt
      481 cgacgagatc gtgcgcgcgg cgcaaagcga ttacgaaagc ctgatcacgg tcaccgtcac
      541 caccgaacgg caggagcgct cggtgtcggg cacggtctat gccgacggca agccgcggct
      601 ggtcgacatc aagggcatcc gggtcgacgc cgagttcggc aaatcgatga tctacgtcac
      661 caacgaggac aagcccggct tcatcggcag cttcgccggc ctgctcggcg cggcccagat
      721 caacatcgcg accttccatc tcggccgcaa caagccgggc ggcgatgcca tcgcgctggt
      781 cgaagttgac ggtccggtgc cggcggcgtt gctcgccaag ttgcagacgc tgccgcaggt
      841 caagcaggcc aaggcgctgg tgttctaaag gcccctcagg aacgcggatg tcgcagtggc
      901 tatgcctgcg atagtcgcga gatgtcgacg tttgatatcc aaccttgcat ccgctcgtcg
      961 ctttttccgg tttcggtgac gatcacgtcc tgacactgcg gctttgcttc catcgctgcc
     1021 tttgcgtcgg cgagcgttcg gtcgatcgca atgtacgcga gggtaccggt aataaagtcc
     1081 ttgaaggttt tgccgacggc ggagacgtaa ggcttatcta gaagttttcc gagattatct
     1141 ctggccggat ctaccggcgg cgcctgcttt gcgccggtat tgagcatttc catccagatc
     1201 gaacgatgga tgattgcaat gcaggcgaat ttatcgttca cgacgagcaa ccgcgatatc
     1261 ttctgaccgt ttggcagcat cttgtcgaag agatcgccga tatccttcaa aagcaaatcg
     1321 tcaattgcct tcgctggcgg gacattcgcc tttacgattg ccgcggccgg catcattttg
     1381 tcggtaacgc gcgtgctcgc caactgttgc gagccggaac gcacggcttc cagcgtgctg
     1441 cgactggcag cttcgtagtt ttctctggtg tagtagaacg cgagtacggt tccgacccag
     1501 gtgccgagta gaggcaaaac ggccgtgaat ataagctgtg cggcatcctt taccgagctg
     1561 ttctggcccc atgcggaggc gcagattacg acaaggccca gcacaataac gccggcggac
     1621 gcccccatca ccaggcgacc gccaagcttg gcacggaata ctgcatcgct gtccattgca
     1681 ccattgctca ctgaagaccc tttccaaggt tcgaccgttt aagtgcatct atcaacgata
     1741 caacgttatg ttgatatggc aataggtgtg gtgtcgctgc ggtggctgcc ctttgcaatc
     1801 ggggccctgc ggcccgccca agcgccttag ggaaacctca atcgaaccaa ttcccttgtc
     1861 gtgacgtctt atcactgggg gcgataggat tttccatgcg tctggtcctg ccggatgatc
     1921 tccggctgtg ccggcgacgc gtggatattt gcgggagaga aatcatgcct attgttgcga
     1981 accgccttgt catgacaagc ctcgtcgttt ttacgacact cgccgcgatg caggccaagg
     2041 cggccaacgt ggaggcgaca tcggcggtcg atgccgtcac catctatccc gatggcgcca
     2101 gcgtcacgcg cgccatcacg gtcgacgttc ccagcgggga taacaccctg gtgatcaagg
     2161 atttcccgct aacgctcgat ccatcctcgt tgcgggtgga gggcgaggcg ggtgccaagc
     2221 ttaccatcgg ggcgatcgat accaggccgc ctcgcgcagc accaccggtc aatctacccg
     2281 aactcgacca gcgcatcgag gcgctgaagg acgagcgcgc caacctgcaa ggcgcgatta
     2341 cggctgccac cgcgcgtcgc aaattcgccg agcgttttgc cgaaacctcg cctgcgggaa
     2401 ttggtgagaa gggtgaggcc cgtccgatcg ccgattggcg cgcggccttt gccgcggttg
     2461 ccgaggaagt agcttccgcg gacgcggcta ttcgcgacgc ggaacgcaag caacgcgaca
     2521 tcgatcgcga gatcgcccgg ctggaatccg atcgcgcgat caagccgcca agcaagctcg
     2581 aggtgaggat cgatctttcg gctgccgccg cgaccaaggc gacgctgcga gggacctatg
     2641 cggtgcgtgc cgcgcgctgg acgccgctct atgacgcccg tctcgacacc ggcgcgaagg
     2701 accgcaagcc cgcgctcgaa ctggtgcgcc gcgccgaaat aacccaaacc acgggagagg
     2761 actggccgaa tattgcgctc agcgtttcga ccgtgcgcag cgcgcgcggc ggcagggcgc
     2821 ctgagctgaa ttcgctgatc gtgcagtatc cgcctccggc gcggccggcg gcgcccgcgg
     2881 gcgctgtgat ggataatgcg aggccgcctg cccaatcacg cgcgatgtcc aaggcgctcg
     2941 atcaacccgc ggaacgagaa aaggccgacg agcaacaggc cgcggcggaa gtcggcggtt
     3001 tccaggtggt gttcaggatt ccgggccgcg tcagcgttgg cgcgagcgag ggcgccaaga
     3061 gcctgcgcgt ctccaccgcg agcatcgcgc ccgatcttgc cgtacgttcg gtcccggtga
     3121 tcgatccgac cgcgtttctc gaagcgagct tcacgcaaaa cgaagacgcg ccgttgttgc
     3181 cgggccgggt cgcgatctat cgtgatggcg tgttcatcgg tcgcagtcac gtggctgccg
     3241 ccggcaagga cgaaacggtg cggctcggtt tcggcgccga tgacaaggtc aaggtcgaac
     3301 gctccgtcgt caaacgcaac gaaggatcgg caggcctgat cctgaccaca tcgaagaccg
     3361 acgaacgctc gttcaagacc gttattcgca atggccatga tttcccgatc aggatcgcga
     3421 tcgaggatca attacccgtc agcgagaatg aagacatcca ggtcgagatg ttgccatcga
     3481 ccacgccgcc ggccgtgacc aatgtacgcg accgtcgcgg cgtgctggaa tgggctttcg
     3541 aggccaaggc aggcgaggtc agggatattg ccttcgcttg gcgagtacgc tggcccaagg
     3601 acaagggcgt cgtcatgacg ccggccggat aatgcgaagc gagcggcggc tggcgaattg
     3661 gcctaacgag aaatgaaaaa tccctcccga aaggggaggg atcttcctgt agtcgccgat
     3721 ggtgggccgg ttgtcttctg gcggttactt cgccttcaga aaatcgccga cctcgagcag
     3781 cactaattcg ttatcgtcgg ccttgttggg atcgcggctc gaggagaacg gcaggttgtt
     3841 gtcgttgccg acaatgatat gcgtctcatc gacacggtcg acgttttcga tg
//
LOCUS       DATNOI010000165         2119 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2470733,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000165 DATNOI010000000
VERSION     DATNOI010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2470733"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VN968_06270"
     CDS             <1..337
                     /locus_tag="VN968_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28878.1"
                     /translation="TKYQRIRDRHERVIIDGICNSACTLVLGIVPMNKICVTPRASLG
                     FHQAYYDKAFTFGIKVTTAAGTSDLMSYYPDAVKDWIRRNGGLTTEMKKIKNGEELWK
                     IVDPCPEEF"
     gene            complement(514..1641)
                     /locus_tag="VN968_06275"
     CDS             complement(514..1641)
                     /locus_tag="VN968_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007792251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HXL28879.1"
                     /translation="MDVRAAVAIAAGKALEISTIQLEGPREGEVLVEIKATGVCHTDE
                     FTLSGADPEGLFPAILGHEGAGVVVDIGRGVTSVNKGDHVIPLYTPECRQCPSCLSRK
                     TNLCTAIRATQGQGLMPDGTSRFSIRGKPIHHYMGTSTFSNFTVLPEIAVAKIRNDAP
                     FDKVCYIGCGVTTGIGAVINTAKVEQGAKAIVFGLGGIGLNVLQGLRLAGAEMIIGVD
                     INNDREPWGERFGMTHFVNPKDLGKDLVPHLVNMTKSGADQIGGADYTFDCTGNVTVM
                     RQALEACHRGWGQSIVIGVAPAGAEISTRPFQLVTGRVWKGTAFGGARGRTDVPKIVD
                     WYMQGKIEIDPMITHVMPLAEINNAFELMKRGESIRGVVTF"
     gene            1744..>2119
                     /locus_tag="VN968_06280"
     CDS             1744..>2119
                     /locus_tag="VN968_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXL28880.1"
                     /translation="MNHNLEHLAARAAPAIFVVLWSTGFIATKYVLHDAEPLTYLTIR
                     MAVVVGLMAVIAAIARPKWPDGIGIAHSVVAGVLVHGFYLGGTAVAIAHSIPAGLSAL
                     IPGLQPILTSTLANRWLGERVTP"
BASE COUNT          391 a          678 c          625 g          425 t
ORIGIN      
        1 gacgaagtat cagcgcatcc gcgatcgcca tgagcgcgtc atcatcgacg gcatctgcaa
       61 ttcggcctgc acgctggtgc tcggcatcgt tccgatgaac aagatttgcg tgacgccacg
      121 cgccagcctc ggcttccacc aggcctatta cgataaagcc tttaccttcg gcatcaaggt
      181 caccaccgcg gcagggacgt ccgacctgat gtcctattat cccgatgcgg taaaggactg
      241 gatccgccgc aacggcgggc tgaccaccga gatgaagaag atcaagaacg gcgaagagct
      301 ctggaagatc gtcgatccct gccccgaaga gttttgattt tcattgctgt cataccccgc
      361 gcgagcgggt atccagcacg ctacggcctt tcgactcaat cactggcact ttgggatact
      421 ggatcgcccg ccttcgctgg cgatgacagg caatcctgga cttgtgcgtc tgcgcaccct
      481 cccggggcac ggcatcacac agcatcgcaa atcctaaaac gtcaccacgc cgcgaatgga
      541 ttcgccgcgc ttcatcagct cgaacgcgtt gttgatctcg gcaagcggca tcacatgggt
      601 gatcatcgga tcgatctcga tcttgccctg catataccag tcgacgatct tgggcacgtc
      661 ggtgcgcccg cgcgcgccgc cgaacgcggt gcctttccac acccggcccg tcaccagttg
      721 aaacggccgc gttgaaatct ccgcgcccgc gggcgccacg ccgatgacga tcgactggcc
      781 ccagccgcga tggcaggctt ccagtgcctg ccgcatcacc gtgacgttgc cggtgcagtc
      841 aaacgtataa tcggcgccgc cgatctggtc ggcgcccgat tttgtcatat tgacgagatg
      901 cgggacgaga tccttgccga ggtcctttgg attgacaaag tgggtcatcc caaaacgctc
      961 gccccacggc tcgcggtcgt tgttgatatc gaccccgatg atcatctcgg cgccggcgag
     1021 ccgcagaccc tgcagcacgt tgagcccgat accacccaac ccaaagacga tcgccttggc
     1081 gccttgctcg accttggcgg tgttgatgac cgcgccaatg ccggtggtga cgccgcagcc
     1141 gatgtagcag accttgtcga acggcgcgtc gtttcggatt tttgccaccg cgatctccgg
     1201 cagcacggtg aagttcgaaa acgtcgacgt gcccatgtag tgatggatcg gcttgccgcg
     1261 gatcgaaaat cgcgaggtgc catcgggcat caggccctgg ccttgggtgg cgcgaatcgc
     1321 agtgcagagg ttggttttgc gcgacagaca tgaggggcat tgccggcatt ctggcgtgta
     1381 gagcggaatg acgtgatcgc ccttgttcac gctggtcacg ccgcgaccga tatcgaccac
     1441 cacgccggcg ccttcgtggc caaggatcgc cggaaacaat ccttcgggat cggcgccgga
     1501 cagcgtgaac tcgtcggtgt ggcagacgcc cgtggccttg atttcgacca gcacttcgcc
     1561 ctcgcgcggc ccctcaagct ggatggtcga tatctccagc gccttgccgg cggcgatagc
     1621 aacggcagcg cgaacatcca tggccattcc ttattctcaa ttttcgctat gtcataccgc
     1681 gcgagccggc ccgcacaaat gggcgttgcc gcacagcata tgatcgggca gtcagcgcgg
     1741 caaatgaatc ataatttaga gcatcttgcc gcacgcgcgg cgccggcgat ctttgtcgtg
     1801 ctctggagta ccggctttat tgctaccaaa tacgtcctgc atgatgcgga accgctgacc
     1861 tatctcacaa tccgcatggc ggtggtggtt gggctgatgg ccgttatcgc cgcgatcgct
     1921 cggccgaaat ggcctgacgg tattggcatt gcccacagcg tcgtcgcggg cgtcctggtc
     1981 catggattct atctgggcgg caccgcggtt gcgatcgcgc attcgatccc cgccgggctt
     2041 tcggcgctga ttccgggcct gcagccgatc ctgacctcga cgctggcgaa ccgctggctc
     2101 ggcgagcgcg tcacgccgc
//
LOCUS       DATNOI010000166        10540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1828200,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000166 DATNOI010000000
VERSION     DATNOI010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10540
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828200"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /gene="rpsI"
                     /locus_tag="VN968_06285"
     CDS             <1..307
                     /gene="rpsI"
                     /locus_tag="VN968_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HXL28881.1"
                     /translation="VNTREVEVYFARPVLRMMIQQPLVAAARAGQYDVICTVAGGGLS
                     GQAGAVRHGISKALTYFEPDLRGILKKGGFLTRDSRVVERKKYGRAKARKSFQFSKR"
     gene            528..1754
                     /locus_tag="VN968_06290"
     CDS             528..1754
                     /locus_tag="VN968_06290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXL28882.1"
                     /translation="MSLDTSTLYLVATMVAATLGAMLLFFGTQENFPALRWWGTAYLL
                     GAVSVALSTLAGNMLGAMLSLALNAVGFAACGMVWNAARVFHGRKPNLPGLLLGAMVW
                     VAAVMTLKPEASAIRFMIGAAIVAIYATLTAAQLWSERRKTLQRRWPAIVVPVLHGFV
                     LMLPILLGGLLRPHDAAFVGSIWVTVFSIELVLYAVATVFVIFMLVSERVVTAHKTAA
                     STDPLTGMFNRRGFAEATSRVIEREANAGRPVTVLIFDLDHFKSINDRFGHSAGDEIL
                     KLFATVVLSTLRITDLSGRVGGEEFAALLPCPLEEGVIAAERVREAFADSGIVVEDGP
                     VDTTVSIGVAGGPAGTELEVLLAAADSALYQAKRNGRNRVEAAEELPLSLEKWRRKTA
                     GLPGHARPPPGVVSPT"
     gene            1925..2161
                     /locus_tag="VN968_06295"
     CDS             1925..2161
                     /locus_tag="VN968_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28883.1"
                     /translation="MDTIPNQSPHAALNSLEAAAVSARGGFACMFSTSDEYETALITE
                     RRAQGRYAPPRSRWPLLVFAGCALIVAGAVLLLN"
     gene            2263..2565
                     /locus_tag="VN968_06300"
     CDS             2263..2565
                     /locus_tag="VN968_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HXL28884.1"
                     /translation="MITEIAQIDVKPGTEKDFEAAVAKARPLFLRAKGGKGFELHKSI
                     EKPSRYRLMAKWDTLENHTVDFRGSEDFAAWRGLVGQYFASPPEVEHTETVLTSAG"
     gene            complement(2586..3716)
                     /locus_tag="VN968_06305"
     CDS             complement(2586..3716)
                     /locus_tag="VN968_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXL28885.1"
                     /translation="MAVSAKDRLAARNDLAWAISVGGIGVVGFAALLLFSWHFAATLF
                     LIFAGMLLGVALNAMTNMLGRVVSLTHSLRLTIVCLVLAGLLSGVVFLGGATIAQQAT
                     ALSGTIKSQLVNVKAFLEKNGIDTSYFDLGNAAAPPTDSSTPAISGAPTTHNLPSAGA
                     IASSGGAIVSQTLKLLLGTVSAVGNFFIVLFLGLTFAAQPGVYRTGLLFMAPVRHRAP
                     ATVIVDRIGETLERWLIAQIITMFAVFLVTWIGLAIIGIQSSFILGIQAGLLGFIPTV
                     GALIGGLIVVLASLASGWVAAASAFVLFLGVHALESYILTPILQRHALDIPPATLFAF
                     QILLGVVFGIWGLALALPLMAIVKVMIDYFKADEIAADAVAA"
     gene            3867..4565
                     /locus_tag="VN968_06310"
     CDS             3867..4565
                     /locus_tag="VN968_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28886.1"
                     /translation="MNFKWVGPLALLAALVIGDQIRINRPGHKYRLMVEVQTPEGIKS
                     ASGVMAVHPDRSYSRGGHTLTRGDAVFIDLGGGKNLLALLAHTDNKSVDLDGMNYLAL
                     RAYNAAGRKVSFNEMSRTTGVVPVKGALVPVLVTFTDPGDPGTARVVLPDETEGALGK
                     GFRVGSISAEVVPNGLWPLDFGGPLGEPVTRGIEAILPWWNGADNPAGKALRAAGLQT
                     GEAIDPKEAFTRKQ"
     gene            complement(4573..5994)
                     /locus_tag="VN968_06315"
     CDS             complement(4573..5994)
                     /locus_tag="VN968_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL28887.1"
                     /translation="MGVLHSLGLRRPGAPQCSADGRFQIAGVTVVNPMHDRRAHATIE
                     VAGGSIAGISDTQAAPPSEFAGCFALPGLVDMHVHLPPDNALKLTQGAALLYMLHGVT
                     SVREAGDLDGTAVAAARTLGTEGVHPVPRVFSCGPFVGAGKATFKNTILLEDASQGAA
                     DAAARRVQAAGASFMKFYEGLTVPMIRALERSCAKHGLKMMGHVPAGITYEDARIAEV
                     QHFFGVPGPQTLERDALVNRSCDWHAVDERRMDAIVDTTLKHGIANTPTIVTNQRMLC
                     YRNFDAARRQPDILKVPPFYLDVIWHPEHGRPSNRMQRDYLERQVVPAIAKKQQLTKK
                     LFDAGAQLFLGTDVAQPFVIPGASLLEEMALFVEAGIGVEQVWKLATRDAGDRLGMRG
                     LGRVETDAPADILLFRHDPTAAIGNISRLEAVVAAGKLYRIADLHRALQSNQAYFTSP
                     LIRPLARRGAERALARALGRSRG"
     gene            6114..6758
                     /locus_tag="VN968_06320"
     CDS             6114..6758
                     /locus_tag="VN968_06320"
                     /inference="COORDINATES: protein motif:HMM:NF037005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL28888.1"
                     /translation="MAKPATDKRTSPRRMPTQARSRARVADILKAAGVLLGEVGYDGL
                     STNLIAERADVPVGSIYQFFEGKDDIVAALVEQFQARILKLVGEQLDAASAMRDHRAF
                     IARLVDGIAGIQAEASAFVCVFSGSQSHARFDGLARDLRRTLTRHLDRVFAEAFPRLP
                     KDDRGRMLAAWSDITGAMISNLDRSKPGERGKLLEELKTVLGAYLDAKLAEFPA"
     gene            6886..7656
                     /locus_tag="VN968_06325"
     CDS             6886..7656
                     /locus_tag="VN968_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HXL28889.1"
                     /translation="MRRPVVGVIGNAYRIEDRFATQLVGERNLRAVAEVSGALPLMFA
                     GSPDITDIGALLDAVDGVVLTGARANVHPTRFRTEPDPRHEPYDLHRDDLALALSEAC
                     VDRGIPLFGICRGLQEMNVAFGGSLHPEIRELPGRMNHRMPRLETGEIHPDPDVVFAD
                     RHDVSLVPGGAFARLFGCETIRVNSLHGQGILEPGGRVVVEGVAEDGTIEAIRIADAS
                     GFALGVQWHAEYDPQHNPINRALFQAFGEALSVRKYRS"
     gene            7830..>10540
                     /locus_tag="VN968_06330"
     CDS             7830..>10540
                     /locus_tag="VN968_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXL28890.1"
                     /translation="MKISIRMPLLFGRQAQNIKGKARQAVSGTSLSTRLGLAMVSLVV
                     VTTAVLSLFTYHSVTEAGIPRALDRLATKAMLGATKLETALNVARQDVMVVQGAPVVT
                     QMGVARAANPFEPVSDKPLRDGIAARFLAALVAKPDYAQLGIIGVADGGRELLRVDRR
                     GPGGTPRIVPEAELVQAGERDYFRRTIGQSKSDVYVSPIALEKVGGRDSPAAPTLHVA
                     IPLLTPSGQVWGISAVDFDLGPKFDRIRAEGGEENQVFVTNAAGDYLLHPDRSREFAF
                     EAGVPVRIQSDFPGFGEALTGGAANDSGIWTDRTGARFGVGWAALRLTGGAGLTILVT
                     ATYSNLNVGLAAVNSSALTGGAIAVLLAIFLAVAIARSLSKPLVQITRAAEGLSRGEL
                     MAMPSNGGREISVLSATFAEMATQIRAKQALLENTIESISDSVLVSDERGQIVVANAA
                     AKQMLEIVPGAGMTRKLNYFYPDGVTPMPASNLALVRALAGESVEDQEFIAVPESPCV
                     PAYIVANARPLKDEFGNLRGAVTVLRNVTEQKRAHQSLVDSEKMAQAIINTALDAFVQ
                     TDETGLILDWSPHAEAMLGWTRSEAVGAKAEDLIVPELQRDANNQWVKQFLHDVGIGA
                     PGWRFEAPLLHRDGREIFTEMSLTELRRGENHIINAFIRDITQKRAAEEQLIQAQKME
                     SVGQLTGGIAHDFNNMLTVITGTIEMLAEAVKGEPHLARIVTLISEAADRGSELTANL
                     LAFARKQPLQPVEIDVNALVNEVGRLLSPTLGRQIEIKSALGGDVWPALVDPGQLSSA
                     LVNLAINARDAMPDGGTLTFATSNIRLNGRDLQGVSGVDRPDDYVVVEVTDTGTGIPE
                     VFRDKIFDPFFSTKETGQGTGLGLSMVFGFVKQSGGNIEVHSEEGHGT"
BASE COUNT         2055 a         3100 c         3390 g         1995 t
ORIGIN      
        1 cgtcaacacc cgtgaggtcg aagtctattt cgcacgtccg gtgttgcgga tgatgatcca
       61 gcagccgctg gtcgcagccg cacgggcggg ccagtacgac gtcatctgca ccgtggccgg
      121 tggcggtctg tcgggccagg cgggcgccgt gcgtcacggc atctcgaagg cgctgaccta
      181 tttcgaaccg gatctgcgcg gcattctgaa aaaaggcggc tttctcacgc gtgactcgcg
      241 cgtggtcgag cgcaagaaat acggccgcgc caaggcgcgc aagtcgttcc agttctccaa
      301 gcgctaaaat tttcagcgaa tattacggat caggaactgc gaacagggcg ccctccggcg
      361 ccctttttgt tgggtatttt ccgaccagtt gacgcagttt ttccggaaaa cttgcgcaac
      421 tgcgcaaaca gatttcaaac acggtcaacg tagatttaca cagaccaaag catgcgctgc
      481 attttcggct gcgccggctt cgctgcatca caatcacccg gtggtccatg tcgctcgata
      541 cctcgacgct ttatcttgtt gcaacgatgg ttgcggcgac gctgggggcc atgctgctgt
      601 ttttcggcac ccaggagaat ttccccgcgt tgagatggtg gggaaccgcc tatctgctcg
      661 gcgccgtgtc ggtcgcgctc tcgacgctcg ccggcaacat gcttggcgcg atgctttcgc
      721 tggcgctgaa tgcggtcggg ttcgcggcct gcggcatggt ctggaatgcc gcacgcgtgt
      781 tccacggccg caaacccaat ctgccggggc tgctgctcgg agccatggtc tgggtggctg
      841 ccgtcatgac gctgaagccg gaagcgtcgg cgatacgctt catgatcggc gccgccatcg
      901 ttgccattta tgcgacgctg acggctgcgc aattgtggtc cgaacgtcgc aagacgctgc
      961 agaggcgctg gccggcgatc gtggtgccgg tgctgcacgg tttcgtgctg atgctgccga
     1021 tcctgcttgg cggcctgctg cggccgcacg acgctgcttt cgtcggcagc atctgggtga
     1081 cggtgttctc catcgagctc gtgctttatg cggtcgccac cgtgttcgtc atcttcatgc
     1141 tggtgtcgga gcgcgtggta accgcgcaca agacggcggc gtcgacggac ccgctgaccg
     1201 gcatgttcaa tcgccgtggt tttgcggagg cgacgtcgcg ggtgatcgag cgtgaggcga
     1261 acgccggccg tccggtgacg gtgctgatct tcgacctcga tcatttcaag tcgatcaacg
     1321 accgtttcgg ccattccgcg ggcgatgaga ttctcaaact attcgccacc gtggtgctca
     1381 gcacgttgcg gatcaccgac ctttcggggc gggtaggcgg cgaggagttc gcggcattgc
     1441 tgccgtgccc gctggaagaa ggggtgatcg cagccgagcg cgtacgcgag gcatttgcgg
     1501 attccgggat cgtggtcgag gacgggccgg tcgataccac cgtcagcatc ggtgtggcgg
     1561 gcgggccggc cggcacagag cttgaggtgt tgctcgcggc cgccgatagc gcgctttatc
     1621 aggccaagcg caatggccgc aatcgcgtcg aggcagccga ggagctgccg ctgtcgctgg
     1681 aaaaatggcg gcgaaagacc gcgggcctcc cgggtcacgc gcgaccgcca ccgggcgttg
     1741 tcagtcccac atagaagcat gtgccggggg ctggacccgt ggatggatgc cggttttcgg
     1801 acaggatcgc gctgcagcga aagaaaaagc gacgcgtttg attgagcgaa agcagggcca
     1861 gactccagcc gcagccccgc ggcggtaacc tgccgtttac tactcggtcc atatcatcgt
     1921 gacgatggac acgatcccca accaaagccc tcacgccgcc ctgaactcgc tcgaagccgc
     1981 agccgtatcg gcgcgcggcg ggtttgcctg catgttctcg acgtcggacg aatatgagac
     2041 cgcgctgatc accgaacgtc gcgcgcaggg tcgctacgcc ccaccgcgca gccgatggcc
     2101 gctgctcgtg ttcgcgggct gcgcgctgat agttgccggc gcagtgctgc tcctgaactg
     2161 aattgccatt ggagcagggg acgccgggcg ccgcgaggca cctttttctt ggcgactgtc
     2221 tgggttagag acttgagaat tctcaaccag aggccgcaac ccatgatcac cgaaatcgca
     2281 caaatcgacg ttaagccggg caccgagaag gacttcgaag ccgcggtcgc caaggcgcgc
     2341 ccgttattcc tgcgtgccaa gggcggcaag ggttttgagc tgcacaagtc gatcgagaag
     2401 ccgtcacgct accggctgat ggccaagtgg gacacgctgg aaaatcacac cgtcgatttc
     2461 aggggatctg aagattttgc ggcatggcgc gggctggtcg ggcagtattt cgcgtcgccg
     2521 cccgaggtcg aacataccga gacggtgctg acctcggcgg gttgaaggac gggcggtggg
     2581 cggtctcagg ccgccaccgc atcggctgcg atttcatcgg ccttgaaata atcgatcatg
     2641 actttcacga tcgccatcag cggcagggcc agcgccaagc cccagatgcc gaacacgacg
     2701 cccagcagga tctggaacgc gaataacgtg gctgggggaa tgtcgagcgc atggcgctgg
     2761 aggatcggcg tgagaatgta gctctccagc gcgtgaacgc cgaggaaaag cacgaaagcg
     2821 ctggccgcgg cgacccaccc ggacgcaagg ctcgcgagca ccacgatcaa cccgccgatc
     2881 agcgcgccga ccgtcggaat gaagccaagc agtccggcct ggatgcccag aatgaaggaa
     2941 ctctggatgc cgatgatcgc gaggccaatc caggttacga gaaaaaccgc gaacatggtg
     3001 atgatttgcg caatcagcca gcgctccagc gtttcgccga tgcggtcgac aatcacggtc
     3061 gcgggtgcgc ggtgccgaac tggcgccatg aacaacagcc ccgttcggta gacgcccggc
     3121 tgggctgcga acgtcaatcc gaggaacaat acaataaaga agttgccgac cgcgctcacc
     3181 gtacccagaa gcaatttcag ggtctggctc acgatcgcgc cgccgcttga agcgatcgcg
     3241 ccggcgctgg gaagattatg tgtcgtcggc gcacctgata ttgccggtgt cgatgagtcc
     3301 gtggggggcg cggccgcatt gccgaggtcg aaatagctgg tatcgatgcc gtttttctcg
     3361 aggaaggctt tgacgttgac caattgcgat ttgatcgtgc cgctcagcgc cgtggcctgt
     3421 tgcgcgatgg tagcgccgcc gaggaacacc acgcccgaca acaggccggc aagcaccaga
     3481 cagacaattg tcagccgcag ggaatgagtg aggctgacaa cacggccgag catgttggtc
     3541 atggcgttga gcgcgacacc gagcagcatt ccggcaaaga tcagaaacag cgtcgcggcg
     3601 aaatgccagc tgaaaagcaa caaagcagca aatccgacga caccgatgcc gccaaccgat
     3661 atcgcccatg ccaggtcgtt gcgggccgca agacgatcct tagccgagac tgccacgttg
     3721 attccttttc cagccatatc cgacagcact ctttagccaa acgcgtccgg gatcaagcgc
     3781 agaaggcgtg ccggtttgac gctggcatat tgattgtgca aagcgatgaa tggaatggga
     3841 tcgcggcatg gcgagaggct gaaagcatga atttcaaatg ggtcgggccg cttgcattgt
     3901 tggcagccct ggtgatcggc gatcagattc gcatcaatcg gcccggtcac aaatatcggc
     3961 tgatggtcga ggtgcaaacg cccgaaggca tcaaatcggc ctccggcgtc atggccgttc
     4021 atcccgacag aagttatagc cgtggcggac ataccctcac caggggcgat gccgtgttca
     4081 ttgacctcgg cggcgggaaa aatcttctcg cgctgctggc gcataccgac aacaagtctg
     4141 tcgatctcga cggaatgaat tatctggcgc tgcgcgccta caacgcggcc ggccggaagg
     4201 tctcattcaa cgagatgagc aggaccaccg gcgtggtgcc tgtgaaaggg gctctggtgc
     4261 cggttctggt cacttttacc gatccgggcg atcccgggac cgcacgtgtg gtgctgcccg
     4321 atgaaacgga aggggcgtta ggcaagggct ttcgtgtcgg ctcgatttca gccgaggtgg
     4381 tgccgaacgg tttgtggccg ctcgatttcg gcggtccgct cggcgagccg gtgacccgtg
     4441 ggatcgaggc aatattgccg tggtggaatg gtgcggataa tccggcgggg aaagcacttc
     4501 gggcggcggg attgcagact ggcgaagcga tcgatccgaa agaggccttc acgcgcaagc
     4561 aatgaccgca aattatcccc gactccgccc gagtgcccgc gccagcgcgc gctcggctcc
     4621 gcgacgcgcg agcggcctga tcagcggcga ggtgaaatac gcctggttcg attgaagcgc
     4681 gcgatggaga tcggcgatgc gatataattt gcccgctgcg accacggctt caagcctcga
     4741 aatgttgccg atcgctgcgg tcggatcgtg ccgaaacaat agaatatcgg ccggcgcatc
     4801 ggtttctacg cgccccagcc cgcgcatacc gagccggtcg cccgcatcgc gcgtcgcgag
     4861 cttccagact tgctcgacgc cgatcccggc ttcgacaaac agcgccattt cctcaagcag
     4921 actggcgccg ggaatgacga agggttgcgc gacatcggtt cccagaaaca gttgcgcgcc
     4981 ggcatcgaag agtttttttg tcagttgctg cttttttgcg atcgccggaa ccacctgacg
     5041 ctccagatag tcacgctgca tgcggttact cgggcggcca tgctcgggat gccagatcac
     5101 gtcgagatag aacgggggga cctttagtat atccggttgt cggcgcgccg cgtcgaaatt
     5161 ccgatagcaa agcatccttt gattggtgac gatggtcgga gtgttggcga taccatgctt
     5221 caacgttgtg tcgacgattg cgtccatccg ccgctcgtcg accgcgtgcc agtcgcagga
     5281 gcgattaacg agcgcgtcgc gttccaaggt ctgcggcccc ggtacgccga agaaatgctg
     5341 cacctcggca atgcgcgcat cttcgtaagt tatcccggcg gggacatggc ccatcatctt
     5401 gagcccgtgc ttggcgcagg agcgctccag cgcgcggatc atcggcacgg tcaaaccttc
     5461 gtagaatttc atgaagctcg cgccggccgc ctggacccgg cgtgcggcag catcggccgc
     5521 cccctggctc gcgtcctcaa gcagaatcgt gtttttgaaa gttgccttgc cggcgccgac
     5581 aaagggaccg cacgagaaca cccgcgggac cggatgcact ccctcggtgc ccaatgttcg
     5641 cgccgcggcg acggccgttc catcgaggtc gccggcttcg cgaacgctgg tcacgccgtg
     5701 cagcatgtag agaagcgctg cgccttgggt gagcttcaag gcgttgtccg gcggcaaatg
     5761 gacatgcatg tcgacaagtc cgggcagcgc aaaacatccg gcaaactcgg atggtggcgc
     5821 ggcctgcgtg tcggaaattc ccgcaatcga gccgccggcg acctcaatcg tcgcgtgcgc
     5881 gcgccggtcg tgcatcgggt tgaccacggt gacgccggct atttggaagc ggccatcggc
     5941 ggagcactgg ggagctccag gtcgtcgcaa tccaaggctg tggagaacac ccattgcaag
     6001 accctccgga tatgccacaa cgctcgcagt agaatcatga atatgagtat atattcatga
     6061 tgttgacaat atgagcagtt attcatattg tcaaccgggc acacggaggc gtgatggcaa
     6121 agccagcgac agacaagcgg accagcccgc gcaggatgcc cacgcaggcg cgcagccggg
     6181 cccgtgtggc tgatattctc aaggcggccg gggtgttact gggcgaagtc ggttacgacg
     6241 gcctctcgac caacctgatc gcggaacgcg ccgatgtccc ggtcggctcg atctatcaat
     6301 tcttcgaggg caaggatgat atcgtcgcgg cgctggtcga gcagtttcag gcgcggatcc
     6361 tcaagctggt gggcgagcag ctcgacgcgg ccagcgccat gcgcgaccac cgggcgttca
     6421 tagcgaggct ggtcgatggc atcgccggca ttcaggcgga ggcgtcggcg ttcgtgtgcg
     6481 tgttctccgg cagccagtcg cacgcgcgat tcgacggcct ggcgcgggac cttcgccgta
     6541 cgctcacccg tcacctcgat cgcgtctttg ccgaagcgtt tccacggctg ccgaaggacg
     6601 accgcggccg gatgcttgcg gcatggtcgg atatcacggg cgcgatgatc tccaacctcg
     6661 atcgcagcaa gccgggagag cgcggcaagc tcctcgagga gctgaagacc gtactcggtg
     6721 cgtatctcga cgccaaactg gcggagtttc ccgcgtgagc aggtcgtttt tccgtctgca
     6781 ttcgacctat gcagacccat ggctgggcgc gcaacgtgtt gatccggcgt cggcggcaag
     6841 gcatgatccg cccgatattt gcggcgtgag attggatagc aggcgatgag gaggccggtg
     6901 gtcggtgtga ttgggaacgc ctatcgcatc gaagatcgat ttgcaacgca gctggtcggt
     6961 gagcgaaatc ttcgcgcggt tgccgaagtg tcgggtgcac tgccgctgat gttcgccggg
     7021 tcgcctgaca tcacggacat cggcgcattg ctggatgccg tcgacggcgt agtgctgacc
     7081 ggcgcccgcg ccaatgttca cccgacacgc tttcgaaccg agccggatcc ccgccacgaa
     7141 ccctacgatc ttcatcgtga cgatctggcg ctggcgttat cagaagcatg cgtcgaccgc
     7201 ggcataccgc tgttcggtat ctgccgcggc ctgcaggaga tgaacgtcgc ctttggcggc
     7261 tcgttgcatc cggagatccg ggagttgccg gggcgcatga accaccggat gccgaggctg
     7321 gagacgggcg aaatccatcc ggacccggat gtcgttttcg ccgaccggca tgacgtcagc
     7381 ctggttcccg gcggcgcctt cgcacggctg ttcggttgcg agaccatccg cgtcaattcg
     7441 cttcacggtc agggcatcct ggagccgggc gggcgggtcg tcgtcgaggg cgtggcggaa
     7501 gacggcacga tcgaggcgat ccgcattgcc gacgcatccg gctttgcgct gggcgtgcaa
     7561 tggcatgcgg agtacgatcc gcaacacaat cccatcaacc gcgccttgtt ccaggcgttc
     7621 ggcgaagcgc tatcggtacg caagtaccgt tcttaaccgt ccacacgttt cgcatcagcc
     7681 cgatagaagc tcaacgtgct tctcggatgt ttttgcacac gattcttatg agtggagacg
     7741 tcaaccgccg caatttgccg gggccgataa ggtattaagc tatggtgact gcgccgccga
     7801 ttgagtgcag ctgaacattc aatctcgtca tgaagatttc cattcgaatg cctttattgt
     7861 tcggccgcca agcgcaaaat atcaagggta aggcgaggca ggcagttagc ggcacctcgc
     7921 tgtcgacccg gctggggctc gcgatggtgt cgctggtggt ggtgacgacg gccgtactga
     7981 gtctcttcac atatcactcg gttaccgagg cggggattcc gcgagcgctc gatcggcttg
     8041 ccaccaaggc aatgttgggc gcgaccaaac tggagacggc gctgaacgtt gcgcgccagg
     8101 acgtcatggt cgttcaaggc gcgcccgtag taacccagat gggtgtggcg cgggcagcaa
     8161 atccgttcga gccagtgtcg gataagccat tgagagacgg cattgctgcg cggtttttgg
     8221 cagcgctggt ggcgaagccg gattatgccc agttgggcat catcggcgtc gctgatggcg
     8281 ggcgcgaact gctgcgcgtc gaccgcaggg ggcccggcgg cacaccccgc atcgttcccg
     8341 aggccgagct tgttcaggcc ggcgagcgcg actatttcag gcgcacgatc ggccagtcaa
     8401 agtccgacgt ttacgtatcg cccattgcac ttgaaaaagt tggcggtcgc gacagtcctg
     8461 cggcgccgac gctgcatgtc gcgataccac tgttgacacc ttccggacaa gtatggggga
     8521 tcagcgcggt cgatttcgat ttaggaccga agttcgatcg cattcgtgcc gagggaggtg
     8581 aagagaacca ggtctttgtc accaacgctg ccggtgacta cctgctgcat ccagaccgaa
     8641 gtcgagaatt cgcgtttgaa gccggggttc cggtgcggat acaaagcgat tttccgggat
     8701 tcggtgaggc gttaacgggc ggagccgcga acgatagcgg aatctggacc gatcggaccg
     8761 gcgcacgatt cggggtgggt tgggcggcgc tgcgcctcac gggcggggct ggactgacga
     8821 ttctggtgac cgccacctat tcaaacctca atgttggtct cgcagccgtc aacagttcgg
     8881 cgctgacagg aggcgcgatt gccgttctgc tcgcgatttt cctcgcggtg gcgattgcaa
     8941 ggtcgttgtc caaaccgttg gtgcaaataa cccgtgcggc tgaaggtctg tcgcgggggg
     9001 aattgatggc catgccttca aacggcggcc gggagatcag tgttctgtcc gcgacgttcg
     9061 ccgagatggc aacgcagatc cgagcgaagc aagcgctgct ggaaaatacc atcgaaagca
     9121 tcagcgattc ggtcctggta tccgatgaac gcgggcaaat cgtggtcgcc aatgccgccg
     9181 ccaaacaaat gctcgagatc gttcctggcg ccggcatgac aagaaagctc aactatttct
     9241 atcctgacgg cgtgacgccc atgccggcct ccaatttggc gctggtgcgg gcactggccg
     9301 gcgaaagcgt tgaagatcag gaattcatcg ctgtgccgga atctccgtgc gttccggcat
     9361 acatcgtcgc gaacgcacgg ccgctgaagg acgaattcgg caatctacgc ggggcagtga
     9421 cggttcttcg caacgtgact gaacagaagc gagcgcacca gtctctggtc gatagcgaga
     9481 aaatggcgca ggccattatc aacaccgcgc tcgacgcctt tgtccagacc gacgagacgg
     9541 gcctcatcct cgactggagc ccgcacgccg aagcgatgct cggatggacc cggtcagagg
     9601 ccgtcggtgc gaaggcggaa gatctgatcg ttcccgagct gcagcgcgac gcgaacaacc
     9661 aatgggtcaa gcaattcctg catgacgtcg gcatcggcgc gccgggctgg cgattcgaag
     9721 cgcccttgct gcatcgggac ggcagggaga ttttcaccga gatgtcgctg acggagcttc
     9781 gtcgcggcga aaaccacatc ataaatgcgt tcattcgcga tatcacccag aagcgcgcgg
     9841 ccgaggaaca actgatccag gcacagaaga tggagtcggt gggtcagctt accggcggca
     9901 tcgcccatga cttcaacaac atgcttaccg ttattacggg aacgatcgaa atgctggctg
     9961 aggccgtaaa gggcgagcct cacctcgcgc ggatcgtgac gttgatcagc gaggcggccg
    10021 accggggatc ggaactcact gcaaatctgc tcgcatttgc cagaaagcag cccttgcagc
    10081 ccgtcgagat cgacgtgaat gcgctcgtga acgaagtggg cagattattg tcgccgacac
    10141 tgggcaggca gatcgagatc aagtcagccc tgggcggcga cgtgtggcca gctcttgtcg
    10201 atccaggcca gctcagttcc gcgctcgtca atctcgccat caacgcccgc gatgccatgc
    10261 ccgacggcgg aacgctgacg ttcgcgacca gcaacatcag gttaaacggt cgtgacctac
    10321 aaggggtcag cggcgtcgat cgtccggacg actacgtcgt cgtcgaggta accgacaccg
    10381 gcaccggtat tccggaagtc ttccgcgaca agatttttga cccgtttttc tccaccaagg
    10441 aaaccggcca aggcacaggg cttggcctca gcatggtgtt tggattcgtc aagcagtccg
    10501 gtggaaatat cgaagttcac agcgaggaag gccatggcac
//
LOCUS       DATNOI010000167        16329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1051068,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000167 DATNOI010000000
VERSION     DATNOI010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 16329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16329
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051068"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VN968_06335"
     CDS             complement(<1..120)
                     /locus_tag="VN968_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXL28891.1"
                     /translation="MSRASSRALKHPPVTAPSEPAGATSPFPELAVAIERKRLR"
     gene            414..2822
                     /locus_tag="VN968_06340"
     CDS             414..2822
                     /locus_tag="VN968_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HXL28892.1"
                     /translation="MPIANLNDSQSSASDLDLLDRYWRAANYLSVGQIYLLDNALLHE
                     PLRPEHIKPRLLGHWGTTPGLNFIYAHLNRAIRASDLNVIYVCGPGHGGPGMVANTYL
                     EGTYSEIYPDISRDADGLRKLFRQFSFPGGIPSHAAPETPGSIHEGGELGYALAHAYG
                     AAFDNPDLIVACVVGDGEAETGPLAASWHSNKFLNPAQDGAVLPILHLNGYKIANPTV
                     LGRMSDDEIRDLFTGYGHEPLFVEGSEPKTMHRLMADTLQTALQGIQAIQKTARDGRK
                     ACDRPQWPVIVLRSPKGWTGPKEVDGLKVEGFWRAHQVPIANPRGNANHLRLLEQWMR
                     SYEPDALFDRTGRLVPELQALAPVGGRRMGANPHANGGLLKRELKLPDFHDYAVDVPR
                     PGGSEAEATRLMGQFLRDVMRLNAETRNFRLMGPDETASNRLDAVFEVTERVWMERIE
                     PYDVHLARDGRVMEVLSEHLCQGWLEGYLLTGRHGFFSCYEAFIHIVDSMFNQHAKLL
                     KVSRGIPWRRPIASLNYLLTSHVWRQDHNGFSHQDPGFVDLVVNKKADIVRVYFPPDA
                     NTLLWIADHCLRTYDRINVIVAGKQPSPQWLTMQEAATHCDAGIGIWAWAGTETAGAE
                     PDVVMACAGDVPTLETLAAVDLLRKSLPDLKIRVVNVVDLMTLQPKEQHPHGTSDRDF
                     DGIFTCDRPVIFAYHGYPHLIHRLTYRRTNHAGMHVRGFGEEGTTTTPFDMVVLNELD
                     RFHLAIEVIERVPGLGVTAAHVKQQFRDALIEHSRYVREHGEDMPQIRDWIWPYGRDA
                     GVSG"
     gene            3182..3751
                     /locus_tag="VN968_06345"
     CDS             3182..3751
                     /locus_tag="VN968_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HXL28893.1"
                     /translation="MLSVIIPTEGLEQPAVATLAALVPGAAAGVVREVLLVDRASTGV
                     IERVADVTGCRFLSFEGSRAAALAAGARQARSPWLMFLHAGAVLDSGWIEETTQFIQG
                     VSNSGRSRAGIFRYARSPYADTSLRDGFKFVARMITGPSADQGLLIARDHYDRLGGYA
                     PDARRSEARLLRQLGRRSRTLLRSRIMVA"
     gene            3828..4757
                     /locus_tag="VN968_06350"
     CDS             3828..4757
                     /locus_tag="VN968_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HXL28894.1"
                     /translation="MSQPDCDDRISAVRAFNRFYTRKLGVLDQQLLKSPFSLSEARVL
                     YELATREDLAAKEIGIELGLDPGYLSRIIQNFDDNGLVTRKPLPSDRRQYRLGLTAKG
                     RQAFAKLDRRSHDDIAGMLATLSRGDAERMIGAMAVIERLLGASRGSSPPAILRDPRP
                     GDMGWVVQSHGSFYASEYGFDASFEGLVAEIAAKFLAAFDASRERCWIADLDGAQVGS
                     VFLVQHTDDVAKLRLLLVDPAGRGQGLGTRLVAECIAFAKACGYRKITLWTQSILVAA
                     RKIYQDAGFVLVAAEPHRSFGQNLIGETWEHEL"
     gene            complement(4962..5525)
                     /gene="moaB"
                     /locus_tag="VN968_06355"
     CDS             complement(4962..5525)
                     /gene="moaB"
                     /locus_tag="VN968_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HXL28895.1"
                     /translation="MSSIDESKKFVPLNIAVLTISDTRSLADDKSGTTLSERLTAAGH
                     RLAAREIVVDDVEAIRDIIKKWIAEPDVDAIITTGGTGFTGRDVTPEAIEPLFEKRMD
                     GFSIAFHLLSHAKIGTSTIQSRATAGVAGATFIFCLPGSPGACRDGWDGILAAQLDYR
                     TRPCNFVEIMPRLDEHLRRPKAKGASA"
     gene            complement(5651..6580)
                     /locus_tag="VN968_06360"
     CDS             complement(5651..6580)
                     /locus_tag="VN968_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HXL28896.1"
                     /translation="MRYDDFRRSDDIDDRRGDDGGGGGGGGGFGLPMGGGLGIGTVIV
                     LGLVGWAFGIDPRILIGGAEILTGGQAPSYQSDRRSAPAKTGAPTDEMGNMISGILGE
                     IDDRWSEIFQASGQTFTGPRIVLFRNVTNGGRCGRAESAMGPFYCPPEKQIFLDTGFF
                     RQVETRFHGCSGNACKFTAAYIIAHEAGHHVQNLLGILPRVTRLQQQAGSKAESNALQ
                     VRIELQADCLSGVWVNREEKKRPGFLEAGDIDAALTTASAIGDDTLQRQATGRVVPDS
                     FTHGSAAQRKQWFMTGYQQGTVKACDTFSAGSL"
     gene            6820..7953
                     /locus_tag="VN968_06365"
     CDS             6820..7953
                     /locus_tag="VN968_06365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009008 - DNA-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HXL28897.1"
                     /translation="MVLSRRGVSARAPRTTFESVSDTRIVVGDCVAEMSKLPAGSVDL
                     VFADPPYNLQLKGDLKRPDESHVDAVNNDWDKFSSFAAYDDFTRAWLLACRRIMKPSA
                     TLWVIGSYHNIFRVGSIMQDLGFWVLNDIVWRKTNPMPNFRGRRFTNAHETMIWAARD
                     EKAKGYTFNYEALKAANEDVQARSDWLIPLCTGDERLKGADGKKVHPTQKPEGLLARV
                     LLSSSKPGDLVIDPFNGTGTTGAVAKRLGRRYIGFERDRTYAAAAEARIAAIEPLPDA
                     TLAPFMTARDAPRVAFSELIERGMISPGARLVDSKKRHGALVRADGAIMLGDKVGSIH
                     RIGAVAQGSGACNGWTFWHIETNKGLRLIDELRAEIRSGMTAG"
     gene            8046..8375
                     /locus_tag="VN968_06370"
     CDS             8046..8375
                     /locus_tag="VN968_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28898.1"
                     /translation="MRRQSEALLLVSLLPIFLVGLFPMLIFALLGFAGLGLLGILLIC
                     AGLSDALDANSDFNQHIIVEGYARRSEQAIHASNLHSAIRSATTMRMAGAALIVASIG
                     GLIYSGL"
     gene            8547..9233
                     /locus_tag="VN968_06375"
     CDS             8547..9233
                     /locus_tag="VN968_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HXL28899.1"
                     /translation="MLKFYFNGAPNPNKVALFLEESGLPYELVPVDTRKGDQFKPEFL
                     KVNPNGKVPAIDDDGVFVFDSNAILLYLAEKTGKFLPPNTPANRAQLLSWLMFVATGV
                     GPYSGQAVHFKHFAPKDLEYAHNRYQFEARRHYGILDDHLANSRYMVGDTYTIVDMAL
                     WGWARLAPFVIGDEVFAKYPNVKRLVDEISARPAAARAIALKDKFVFKAEMDDEARDI
                     MFGHLKTKAA"
     gene            complement(9281..9694)
                     /locus_tag="VN968_06380"
     CDS             complement(9281..9694)
                     /locus_tag="VN968_06380"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXL28900.1"
                     /translation="MSKPSREFLSPKTLMPPAGYSHIAKVNSGTTVYLAGQVSSDASG
                     KLIGEGDFEAQVEQVFRNLRIAVETAGGTMADIVKLNIYLVSEVDQADVPKMRAIRDR
                     YVNVEKPPASTLVVVSRLARPGWLIEIEAVAAIDD"
     gene            complement(9765..10190)
                     /locus_tag="VN968_06385"
     CDS             complement(9765..10190)
                     /locus_tag="VN968_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXL28901.1"
                     /translation="MGATENRKLMESIFARVAVGDRSALVDALADDVTMRVTGQYSWS
                     RTFTGKATLLRELYGYLNTLLAEGRRTIPLRFIADDDHVVVEARGDMKTKAGVRYDNE
                     YCLIYRLKDGRIVEIREYCDSVLTEAVLGKFPEPAKAAG"
     gene            10283..11200
                     /locus_tag="VN968_06390"
     CDS             10283..11200
                     /locus_tag="VN968_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL28902.1"
                     /translation="MNLTALDLNLLVALDALLLEANVSRAAMRIGLSQPAASHALQRL
                     RDVLGDPLLVRVGARMELTPRAQTLRGPLAQVLDQVRGLLIPDDFDAASSERQFRLMM
                     PDLAVELLVPPLMEKIAALAPNVRLDIVPWRGSAIITAEFARTIDLVISIGNAFKGFH
                     RQLLYTDRDALAVRRGHAIGAKLKRRDVFLNARHVAVVIRGQNEDLIDGWLRAKGLER
                     RVALVVPGYIEALHIVARTDLVAFVPGRLIAALTKPLSLTTVTPPLDPGIDEQFMFYP
                     TRAQMDPGSIWLRKLMLETGRGLDRRKAA"
     gene            complement(11217..11354)
                     /locus_tag="VN968_06395"
                     /pseudo
     CDS             complement(11217..11354)
                     /locus_tag="VN968_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611976.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11391..12392)
                     /locus_tag="VN968_06400"
     CDS             complement(11391..12392)
                     /locus_tag="VN968_06400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017957677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HXL28903.1"
                     /translation="MKSFLIDRYTKGGALRLGESPEPELRNNDVMVEIHGAGVNVLDN
                     KIRDGEFKLILPYRLPLALGNDVAGVVVRIGANVRQFKPGDEVYARPAQDRIGTFAEY
                     IAMDEADVAMKPNNLTMEEAASIPLVALTAWQVLVERANLQKGQKVLIHAGSGGVGTI
                     AIQLAKHLGAYVATTTSTANIALVKSLGADVVVDYKKDDFEKVLQGYDVVLNSLGKDT
                     LEKSLAVLKPGGKLISISGPPDPDFARENGSGWLLQQVMRLLSSGIRRKSKRRGISYS
                     FLFMTANGGQLGKITSLIEAGNIRPVMDRVFPFEKTNEALAYVETGRAKGKVVVTVK"
     gene            complement(12451..13258)
                     /locus_tag="VN968_06405"
                     /pseudo
     CDS             complement(12451..13258)
                     /locus_tag="VN968_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017996926.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            13348..13779
                     /locus_tag="VN968_06410"
     CDS             13348..13779
                     /locus_tag="VN968_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007795032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL28904.1"
                     /translation="MKNLSSQPCLIARSLALVGDAWSMLIMRDAHAGLTRFDDFRKSL
                     GIAPTMLTGRLSALTDEGLLEKRRYSDRPPRDEYVLTEAGRDFLPVLFAIGAWGRKHR
                     GGGKVTRFFDADTGTEIDPVTIDRETGAPVGTRPIRVAAPE"
     gene            complement(14014..14634)
                     /locus_tag="VN968_06415"
     CDS             complement(14014..14634)
                     /locus_tag="VN968_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HXL28905.1"
                     /translation="MLKLYYAPGSCALASHIALQEAGATYTTERLDFKSNQQNSPEYL
                     AVNPKGRVPALITDRGILTETPAMLAFIAQSFPQAKLAPFDDAFAFAQVQAFNSYLCS
                     TVHVAHAHKGRGYRWATDEASFADMKHVVPKSVGAGFALIERNMLKGPWVMGETYTIC
                     DPYLFTLAGWLEGDSVDLSTLPKVADHRKRMSERPAVQKVLAEENS"
     gene            complement(15023..15499)
                     /locus_tag="VN968_06420"
     CDS             complement(15023..15499)
                     /locus_tag="VN968_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXL28906.1"
                     /translation="MQLAQLNHPSAETMNELRALNARFIHNFVTNDVASHDAILHPAF
                     INIWPTGQRWDRATYLKYWATAFDPKIIVYWDVRDELITVIGDVALVRATNKHTRRRD
                     GQEVTGMTMYTDTYLFENGAWKCIQAQLTPVAPEHQPADDTIISVYIEGKRQERLS"
     gene            complement(15519..>16329)
                     /locus_tag="VN968_06425"
     CDS             complement(15519..>16329)
                     /locus_tag="VN968_06425"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL28907.1"
                     /translation="SNTHDVATGHFSSLLKHWRNIRRLTQIELAGDANVSARHLCFLE
                     TGRAQPSREMVQLLGSALDLPLEECNALHVAAGFVPPYGDRGLAAENLQPVRQALDFI
                     LRQQEPYPGIVIDGRWDVRIRNQASRRLFKAFHQSYEMEADVAENAMHVVFHPKGLRQ
                     FITNWDEFAGQMIQILHREVAQGGRVAAQLLDEIMVYPGLPAEWRLPRNPAGSSPVMA
                     MQLRKGDFCLAFFSTFTTLAMPMDAALQKLKIECFYPADSATAEKARELAL"
BASE COUNT         3079 a         5166 c         4849 g         3235 t
ORIGIN      
        1 acgaagccgc tttcgttcga tcgcgacggc caattcagga aaaggagaag tcgcacccgc
       61 cggttcggag ggcgccgtga ccggcgggtg cttgagggca cgagaggatg ctctgctcat
      121 ggtgagaaca tagcacgcaa cgagaacaaa tcaagaacat attcaacgaa gaccgaattt
      181 caccatccgc tcgctcggcc ggccactttg ttgaggggaa tcaataccag ccaggtttgt
      241 ccgaggcagg attggcgatg gcagcgcgta tcagggaaca tgtgggcagc ccccgggttc
      301 gaccaccgcc aactgacgcg gtctctacac aatcaaagcc catcctgttt cacatcagcc
      361 gggatcgatc agggctcgat tttgttgcgc atcatcatcc ctcggaaatc agcatgccaa
      421 tcgcaaacct gaacgatagc cagtcaagcg ccagcgatct ggatttactc gaccgctact
      481 ggcgcgctgc gaattatctg tcggtcggtc agatctatct gctcgacaat gccctgcttc
      541 atgaaccatt gcgcccggaa cacatcaagc caaggctgct tggacattgg ggaaccacac
      601 ccgggctcaa ttttatctat gcccatctca atcgcgcgat tcgggcatcc gacctgaacg
      661 ttatttatgt gtgcgggccc ggccacggcg gaccggggat ggtcgccaac acctatctcg
      721 aaggcaccta cagcgagatc taccccgaca tcagccgcga tgccgatggc ctgcgcaaac
      781 tattccgcca gttctcgttt cccggcggca tcccgagcca cgcggctccg gaaacgccgg
      841 gatcgattca tgagggcggc gaactcggct acgcgctggc gcacgcctat ggcgcggctt
      901 tcgacaatcc ggacctgatc gtcgcctgcg tggtgggcga cggcgaagcc gagaccgggc
      961 cgctggccgc ctcctggcac tccaacaaat tcctcaatcc cgcgcaggac ggcgcggtgc
     1021 ttccgatcct tcacctcaat ggctacaaaa tcgccaatcc cacggtgctc ggccgaatga
     1081 gcgacgatga gatccgcgat ctcttcacag gctacggtca cgaaccgctg tttgttgaag
     1141 gcagcgagcc caagacgatg catcggctca tggccgacac gctgcagact gcgctgcagg
     1201 gcatccaggc cattcagaag accgcacgcg acggacgcaa agcctgcgat cgtccgcaat
     1261 ggcctgtcat cgtgctccgc agtcccaaag gctggaccgg cccgaaggag gtcgatggct
     1321 tgaaggtcga aggcttctgg cgcgcgcatc aggtgccgat cgccaacccg cgcggaaatg
     1381 ccaatcatct caggctgctc gagcaatgga tgcgcagcta tgagcccgac gcgctgttcg
     1441 acaggaccgg ccgcctcgtc ccggaattgc aggcgcttgc acccgtcggt gggcggcgaa
     1501 tgggcgccaa tccgcacgcc aatggcgggc tgctgaagcg cgagctcaag ctgccggact
     1561 ttcatgatta tgcggtcgat gttcccaggc ccggcggctc cgaggccgag gcgacgcgct
     1621 tgatggggca attcctccgc gacgtcatgc ggctgaacgc cgagacgcgc aatttccgat
     1681 tgatgggacc ggatgagacc gcctcaaatc gcctcgatgc ggttttcgag gttaccgaac
     1741 gggtctggat ggagcgcatt gaaccctacg acgttcacct cgcccgggat ggccgggtga
     1801 tggaagtcct gagcgaacat ctctgccagg gctggctgga aggatatctg ctcaccggac
     1861 ggcacggctt cttctcgtgt tacgaggcct tcatccacat cgtggattcc atgttcaacc
     1921 agcatgccaa attgttgaag gtctcgcgtg gcatcccctg gcgacgcccg atcgcgtcgc
     1981 tcaactatct cctgacgtcg catgtctggc ggcaggacca caacggcttc agccatcagg
     2041 accccggctt cgtcgatctc gtcgtcaaca aaaaggccga catcgtccgc gtgtattttc
     2101 cgccggatgc caacacattg ctctggatcg ccgaccattg cttgcggacc tacgaccgga
     2161 tcaacgtcat cgtcgccggc aagcagccgt cgccgcaatg gctgaccatg caagaggccg
     2221 cgacgcattg cgacgccggc atcgggatct gggcgtgggc cggcaccgaa accgcgggcg
     2281 ccgaacctga cgtcgtgatg gcctgtgccg gtgacgttcc aacgctggag acgctggccg
     2341 ccgtcgatct gcttcgcaaa tccctgcccg acctgaagat ccgggtcgtc aacgtcgtcg
     2401 atctgatgac cctgcagccg aaagaacagc acccgcacgg caccagtgat cgcgatttcg
     2461 acggaatatt cacctgcgat cgaccggtga tctttgccta tcacggctat ccccatttga
     2521 tccatcgtct gacctaccgt cggaccaatc acgccgggat gcacgtccgc ggtttcgggg
     2581 aagagggcac cacgacgacg ccgttcgaca tggtcgtctt gaacgaactc gaccgcttcc
     2641 atctggcgat cgaagtcatc gagcgcgtgc ctggcctcgg cgtcacggcg gcccacgtca
     2701 aacagcaatt tcgcgacgcc ctgatcgagc attcgcgcta tgtccgggag cacggcgaag
     2761 acatgccgca aatccgcgac tggatctggc cctatggcag ggatgcgggc gtctccggct
     2821 aaagcgtttt caagcgaagc atgcctggac gtgatccgga gggagagacc ggttcgcgtc
     2881 aggaaaacgc gtcaaatcag gaatccagaa ccggtttcga ttccatcgaa acggaaaagg
     2941 ccataggggt cggcgaccgc gattgccccg gaaggccaaa atccgccacg catcgccagg
     3001 ctcaccatgg atcaaccggc ggacaggcga cattatctgt agcccgccgt tgctcgatag
     3061 tgcctcgaac cctcccaccc ccgcgattgc cgcaaaaagc tgcaatcggc gggctgattt
     3121 gattgtgaca aggtgataac ggtcggcgtt ttctctcctg tgaggcataa tagaaccgtc
     3181 aatgctgagc gtcatcatcc ccaccgaagg cctggaacag cctgccgtcg ccacccttgc
     3241 cgcgctggta cctggtgctg ccgccggcgt ggtgcgcgag gtgctgctgg tcgaccgggc
     3301 gagcaccggc gtgatcgagc gcgtcgccga tgtaacgggg tgccgcttcc tgtcattcga
     3361 gggatcgcgc gccgcggcgc tggccgcggg cgcccgccag gcccgctcgc cctggctgat
     3421 gttcctgcac gccggcgccg tgctggatag cggctggatc gaggagacca ctcaatttat
     3481 ccagggcgtc tcgaacagcg gccgttcccg cgccgggatc ttccgctacg cccgctcgcc
     3541 ctatgccgac acaagcctgc gcgatggttt taaattcgtc gcccggatga tcacagggcc
     3601 ttccgccgac cagggattgc tgatcgcgcg ggaccattac gaccggctcg gaggctatgc
     3661 gccggacgcc cgccgctccg aggccagact gctccgacaa ctcggccgcc gctcgcgcac
     3721 gctgctgcgc agccggatca tggtggccta ggcgctttgc gatactctcc cgatatactt
     3781 gccaaagtca aagaattatt tgacaatggc aactatcgag gtgcgccatg tcccagccag
     3841 attgcgatga ccggatatcg gcggtgcgcg ccttcaaccg cttctatacc cgcaagctcg
     3901 gcgttctcga ccagcagctg ctgaaaagtc cgttctcgct gagcgaggcg cgggtgttgt
     3961 acgaactcgc tacccgggaa gatctggccg ccaaggagat cgggatcgaa ctaggccttg
     4021 atccaggcta cctcagccgg atcatccaga atttcgacga caacgggctg gtcacccgca
     4081 agccgctgcc ttcggaccgg cggcaatacc ggctcggtct gaccgcgaaa ggccgacagg
     4141 ccttcgcgaa gctcgatcgc aggtcgcacg acgacatcgc cggcatgctg gccacgctgt
     4201 cacgcggcga cgccgaacgg atgatcgggg cgatggccgt gatcgaacgg ctgctcggcg
     4261 catcgcgcgg ttcatctcca cccgcgatcc tgcgcgaccc ccgtccgggc gacatggggt
     4321 gggtggtgca gagccatggt tcattctatg ccagcgaata cggcttcgac gcttcgttcg
     4381 aaggcctggt ggcggagatc gcggccaaat tcctcgccgc gttcgacgcg tcgcgggaac
     4441 gctgctggat cgccgacctc gatggcgccc aggtcggctc ggtctttctc gtccagcaca
     4501 ccgacgatgt cgccaaactg cggctgctgc tggtcgatcc ggccggacgc ggccaaggac
     4561 tgggcacacg gctcgtcgcc gaatgcatcg cgttcgcgaa ggcctgcggt taccgcaaaa
     4621 tcacgctgtg gacccagagc atcctggtcg ccgcccgcaa gatctatcag gatgccggat
     4681 tcgtcctggt ggccgccgag ccgcaccgca gcttcggcca gaacctgatc ggcgagacct
     4741 gggaacacga gctctagcgt tagttcaatt agtcacttcg tcattccggg atggtcccaa
     4801 cgggtcggcg ctttgcgccg cccgatgaca ggctctggac cagacctcag atgtgcaatt
     4861 gcacatcggg gaatctcgag attccgggtt cgcgtcggag cctgtcatcg ggccgcgctt
     4921 cgcgcggacc cgttggacgc gccccggaat gacaaaccgg atcaggccga cgcacccttc
     4981 gccttcgggc ggcgcagatg ttcatcgagc cgcggcatga tctccacaaa attgcagggc
     5041 cgggtgcggt aatcgagctg ggcggcaaga atgccgtccc agccgtcgcg gcaggcgccc
     5101 ggcgatcccg gcaggcagaa gatgaaggtc gcgcccgcga cgcctgcggt agcgcggctc
     5161 tggatcgtcg atgtgccgat cttggcgtgg ctcagcaggt gaaacgcaat ggaaaaccca
     5221 tccatccgct tttcgaacag cggctcgatc gcctccggcg tcacgtcgcg gccggtgaag
     5281 ccggtcccgc cggtggtgat gatggcatcg acatcaggct cggcgatcca tttcttgatg
     5341 atgtcgcgga tcgcctcgac atcgtcgacg acgatttcgc gtgccgcaag ccggtgaccg
     5401 gccgcggtaa gccgctccga caacgtggtg ccggatttgt catccgccag cgagcgcgtg
     5461 tctgaaatcg tcagcaccgc gatgttgagc ggcacgaact ttttcgattc atcgatggaa
     5521 gacatgacca tgcctcttct cgtcatgccc ggcctcgtgc cgggcatcca cgtcttcaat
     5581 atccctcaaa agcaaagacg tggatggccg ggacgagccc ggccatgacg atcaatgctt
     5641 agcaataccg tcacaacgaa cccgcgctga aggtatcgca ggccttcacc gtgccctgct
     5701 gatagccggt catgaaccat tgctttcgct gcgcggcgga accgtgggta aacgaatccg
     5761 gcaccacccg cccggtcgct tgccgctgca gcgtatcgtc gccgatcgcc gatgccgtgg
     5821 tcagcgccgc atcgatgtcg ccggcttcga ggaagcccgg acgtttcttc tcttcgcggt
     5881 tgacccagac ccccgacagg caatccgcct gcaactcgat cctcacctgc agcgcattgg
     5941 attccgcctt gctgccggcc tgctgctgca gccgcgtcac gcgcggcagg atgccaagca
     6001 gattttggac atgatggccg gcttcatggg cgatgatata ggccgcggtg aatttgcagg
     6061 cattgccgga acagccgtga aaccgcgtct cgacctggcg gaaaaatccg gtgtcgagaa
     6121 aaatctgctt ttccggcggg caatagaacg gccccatcgc cgactccgcg cggccgcagc
     6181 gtccgccatt ggtgacgttg cgaaacaaca cgatacgcgg accggtaaag gtttgcccgc
     6241 tggcctggaa gatctcgctc cagcgatcat cgatctcgcc gaggatgccg gagatcatgt
     6301 tgcccatctc gtcggtcggc gcaccggtct tggcgggcgc ggacctgcga tcggattgat
     6361 agctcggcgc ctgtccgccg gtgagaattt cggcgccacc gatcaggata cgcggatcga
     6421 tgccgaaggc ccaaccgacg aggccgagca cgataacggt gccgatgccg aggccgccgc
     6481 ccatcggaag accgaagccg ccaccaccgc caccgccgcc gccatcatcg ccgcgacgat
     6541 catcgatgtc gtcgctgcgg cgaaagtcat cgtaacgcat ggtggttttt cctcattccg
     6601 gccggctggc agcgcgctcg ccgataccca acgtgccaac cacgtaaaat ttccattcga
     6661 taaatcaata acccgcccgg caaaaattgc ctagccatca atgcctagca aacgcgccgc
     6721 gataacccgg tgtttacctc ggcacgtcaa ggtatggcca gtcggttgtt ttagtcccgc
     6781 gtcgccgaca gcgtcgcctg agttcagagt attcgagtca tggtattgtc gcgtcgcggg
     6841 gtgtctgcaa gggcgccccg caccacattc gagtcggttt cggatacccg tatcgttgtc
     6901 ggcgattgcg tcgccgagat gtcgaagctt ccggcaggct ccgtcgacct ggtgttcgcc
     6961 gatccgccgt acaatttgca gctcaagggc gatctcaagc gccccgacga atcccatgtc
     7021 gacgccgtca acaacgactg ggacaagttc tcgtccttcg ccgcctatga tgatttcacc
     7081 cgcgcctggc tcctggcctg ccgccgcatc atgaaaccgt cggcgacgct gtgggtgatc
     7141 ggctcctacc acaacatttt ccgcgtcggc tcgatcatgc aggacctggg cttctgggtt
     7201 ctcaacgata tcgtctggcg caagaccaat ccgatgccga atttccgcgg ccgccgtttc
     7261 accaatgccc acgaaaccat gatctgggcg gcgcgcgacg agaaggccaa gggctatacc
     7321 ttcaactacg aggccctgaa ggccgccaac gaggacgttc aggcgcgttc ggactggctg
     7381 attccgctct gcaccggcga cgaacgcctt aaaggcgccg acggcaagaa ggtgcacccg
     7441 acccagaagc ccgaaggcct gctggcgcgc gtgctgctgt cgtcgtcgaa gcccggcgat
     7501 ctcgtgatcg atccattcaa cggcaccggc accaccggcg ccgtggcaaa acgtctcggc
     7561 cgccgctata tcggcttcga gcgcgatcgg acctatgcgg ctgcggcaga agcgcgcatc
     7621 gccgccatcg aaccgctgcc ggatgcaaca ttggcgccat tcatgaccgc gcgcgacgcg
     7681 cctcgcgttg cgttttccga actgatcgag cgcggcatga tctcgcccgg cgccagactg
     7741 gtcgactcca aaaagcgcca cggcgcgctg gtgcgtgccg acggcgccat catgctcggc
     7801 gacaaggtcg gctcgatcca ccgcatcggc gcggtggcgc agggttcggg tgcctgcaac
     7861 ggctggacct tctggcacat cgaaaccaac aagggtttgc gcctgatcga cgaactgcgc
     7921 gccgaaatcc gctccgggat gacggcgggc tgagatccgg cggccggtac acctttcatc
     7981 ttgattccac acggtggcgg ggactaaact cgacacacgc cgttgactgc gtcgagcgat
     8041 ctccgatgcg aaggcaatct gaagcactgc tgctggtgtc gctgctgccg atctttctgg
     8101 tcggcctgtt tccaatgctg atctttgcgc tgttgggatt tgccggtctc ggcctgctgg
     8161 gcatcctgct gatttgcgcg ggcttgtccg atgcgttgga cgcgaacagc gacttcaatc
     8221 agcatattat cgtcgagggt tatgcgcgcc gatccgaaca ggcgatccac gcgtcaaatt
     8281 tgcattcggc catccgttca gcgaccacca tgagaatggc cggcgccgcg ctgattgtcg
     8341 caagcattgg cggcttgatt tattccggat tgtagagtgg gaaggcgcgc ccgctgctcc
     8401 atgcaatgac ggcgccgcat accttgctca cgaactcgtc gcttgcgctt tttgcgaaaa
     8461 tttgggcatg gacatgccgc ctccccgcgg tatcgtcatc acgtcccgcc ttttctcggt
     8521 aacaaagaag caaggagaga attccaatgc tcaaattcta cttcaacggc gcgccgaacc
     8581 ccaacaaggt cgcgctgttt ctggaagagt ccggccttcc ttacgaactg gtgccggtcg
     8641 atacccgcaa gggcgatcag ttcaagccgg aattcctgaa ggtgaatccc aacggcaagg
     8701 tgccggcgat cgacgatgac ggcgtgttcg ttttcgacag caacgcgatc ctgctttatc
     8761 tcgccgagaa gaccggcaaa tttctgcctc ccaacacgcc tgccaaccgt gcgcagttgt
     8821 tgtcttggct gatgtttgtc gccaccggcg tcggcccgta ttccggccag gcggtgcatt
     8881 tcaagcactt cgcgccgaaa gacctggaat atgcccacaa ccgctaccag ttcgaggccc
     8941 ggcgtcacta cggcattctc gacgatcacc tcgctaacag tcgttacatg gtcggcgata
     9001 cctacaccat cgtcgatatg gcgctctggg gctgggcgcg tctcgcgccg ttcgtgatcg
     9061 gcgatgaagt gtttgcgaaa tatcccaacg tcaagcggct ggtcgatgaa atctcggcaa
     9121 gaccggccgc ggcgcgggcg atcgcgctga aagacaagtt cgtcttcaag gccgaaatgg
     9181 acgacgaggc gcgcgacatc atgttcggcc atctcaagac caaggcggcc tgatcaaacc
     9241 aggacggcag aacggcgccc ttcgcggcgc cgttcttgtt tcagtcgtcg atcgccgcga
     9301 ctgcctcgat ctcgatcagc cagcccggtc gcgccagtcg gctgacgaca accagcgtgc
     9361 tcgccggcgg cttctccaca ttgacgtagc ggtcgcgtat cgcgcgcatc ttcggcacat
     9421 cggcctgatc gacctccgag accaggtaaa tgttcaattt gacgatatcg gccatggtgc
     9481 cgccggcggt ctcgaccgca atcctgagat tgcggaacac ctgctcgacc tgcgcctcga
     9541 aatcgccctc gccgatcaac ttgccggacg cgtcgctcga gacctggccg gccaaataga
     9601 cggtcgtccc gctattgacc ttggcgatat gcgaatatcc cgccggcggc atcaaggtct
     9661 tcgggctgag gaattccctt gatggcttgc tcatgtcgat ctcccctgtc acttcagcca
     9721 gaacggcttg ttgagttcgt ccgctatttc cggccggcag atacctagcc cgccgctttc
     9781 gcgggctctg gaaactttcc aagcaccgct tcagttagca ccgagtcgca atattccctg
     9841 atctcgacga tcctgccgtc tttcaggcgg tagatcaggc aatattcgtt gtcgtagcgg
     9901 acacccgcct tggtcttcat gtcgccccgc gcctcgacca cgacgtgatc gtcgtcggcg
     9961 atgaaccgaa gcgggatggt gcgccgtcct tccgccaata gcgtgtttag atagccatac
    10021 aactcgcgca gaagcgtcgc ttttcccgtg aacgtcctcg accacgaata ttggccggtt
    10081 acgcgcatcg tgacgtcatc ggccagagcg tcgacgagcg ccgagcgatc gccgaccgcg
    10141 acacgcgcaa aaatgctctc catcagtttc ctgttttcgg tggcgcccat ggcgaattct
    10201 ccttcaacag cttgatgggc ccatcttcgc cggtcgcacg tcattcttcc aatagataaa
    10261 acatatgata tatattcagc tgatgaattt gaccgcgctc gatcttaacc tgctcgtcgc
    10321 gctcgacgcg ctgctgctgg aggcgaatgt cagccgcgcc gcgatgcgga tcggcctgtc
    10381 gcagccggcg gcgagccatg cgctgcagcg gctacgcgac gtgctcggcg atcccttgct
    10441 ggttcgcgtc ggcgcgcgaa tggaattgac gccgcgcgcc cagacgctgc gcggaccgct
    10501 ggcgcaagtc ctcgatcagg tgcgcgggct gctcattccg gacgatttcg atgccgccag
    10561 cagcgagcgg cagtttcgcc tgatgatgcc cgacctcgcg gtggaacttc tggtgccgcc
    10621 gctgatggag aagattgcag cgttggcacc gaacgtccgg ctcgacattg tgccctggcg
    10681 cggttcggcg atcatcaccg cggagtttgc acgcacgatc gatctcgtga tctcaatcgg
    10741 caatgccttc aagggatttc accgtcagtt gctctatacc gaccgtgacg cgctcgcggt
    10801 acggcgcggc cacgccattg gcgcaaagct gaagcggcgc gatgttttcc tcaacgcgcg
    10861 gcacgtcgcc gtcgtcatcc gcggtcagaa cgaagacctg atcgacggct ggctgcgagc
    10921 caaagggctg gagcgacgcg tagcgctggt cgtgcccggc tatatcgagg cgctccatat
    10981 cgtcgcgcgc accgatctcg tggcatttgt tcccggtcgg ctgatagccg ccctgacaaa
    11041 accgttgtcg ctgacgacgg tcacgccacc actcgacccc gggatcgacg aacagttcat
    11101 gttctatccg acccgcgccc agatggaccc aggttcgatc tggctgcgca agctgatgct
    11161 ggagaccgga cgcgggctgg acaggcgcaa ggctgcctag gctcgcgact cactaatcac
    11221 agccagaaga tgacggctgc cgcaatgcat gtgcttgaga agaagacgtg tgcgcagcgg
    11281 tcatatcgag tgtgaatacg acgccagtcc ttgagttttc caagcatgtt ttcgattttg
    11341 tggcgttttc tgtacagcgc aggatcatgg cccctattgg cccccaagcg tcacttcaca
    11401 gttacgacga ctttgccctt cgcacgaccc gtttcgacat aggccaatgc ctcgttggtc
    11461 ttctcgaacg ggaagacccg gtccatcact gggcgtatgt tacctgcctc gatcagggag
    11521 gtgatctttc cgagttgccc gccgttcgct gtcatgaaga gaaacgaata gctgatccct
    11581 cggcgtttcg attttctcct gatgccggaa ctcagcaggc gcatgacctg ttgaagcagc
    11641 cagccggagc cgttctccct cgcgaagtcc ggatcgggcg gaccggaaat cgagatcagc
    11701 ttcccgcccg gcttcagcac agcaagggat ttctccagcg tgtccttccc gaggctgttc
    11761 agcacgacgt catagccctg cagcaccttc tcgaagtcat ccttcttgta gtcgaccacg
    11821 acgtctgcgc cgagactttt cacaagagcg atatttgctg tgctcgtggt cgtggccaca
    11881 tatgccccga gatgcttggc cagctggatc gcgatcgtgc ccacgccgcc cgagccggca
    11941 tgaatcagga ccttctgccc cttctgcaag ttggcccgct cgacaagcac ctgccatgct
    12001 gtcagggcga caagcggaat ggatgcggcc tcttccatgg tcagattgtt cggcttcatc
    12061 gctacatcgg cctcgtccat ggcgatatac tctgcaaatg tcccgatgcg atcctgggcc
    12121 ggacgtgcat agacctcgtc accgggcttg aattgccgga cattggcccc gatccggacc
    12181 acgacgcccg ctacatcgtt gcccagcgcc agtgggaggc ggtagggcag gatgagcttg
    12241 aactctccgt ccctgatctt gttgtccagg acgttcacgc cggcgccatg aatctcaacc
    12301 atgacgtcat tgttccgcaa ctccggctct ggactttcgc cgagccgcag ggcgccgcct
    12361 ttcgtatagc gatcgatcag gaatgatttc atcagactga ctttcgtttc tgtggtgagc
    12421 cggcagcgcc ggcaatgcgg cgatgaaagc tcaatcggga agtctgctga atttccgaag
    12481 gttcttgtcg acgaaggact cgggcaggaa gcggcgcatg aagcggacct gtcctgcagc
    12541 ttttccggca gtatttgggc gaggccgcgc tggcggccct tacgacggct ttggcgacca
    12601 cctcgggtgc atcgcccttc gcgacgatct cgcgcatcag cttctccgcg tcggcgcgaa
    12661 ccgtatcgta gacgacaagc ggccggtccg gtcgcgtgat gttctcttcg aaggacgtgc
    12721 gggtcacgcc gggctcgaca agcacgatgc ggatgccctg cgttcgcact tcgtggtcga
    12781 gagattccga atagccttcg atggcgtgct tggtcgatgc gtagagtgcg ttatagggag
    12841 cggggatcag cccgagtatg gaactgaggt tgacgatccg gcctctacgc tgccgtctca
    12901 tcactggcag aaccgcattt gtcattcgta tgataccgaa cacgttcacg tcgaatacgg
    12961 ctttcgcctg tgacgtcgtg gattcctcag cgccgcccag cagtccgatc ccggcattgt
    13021 tgacgagaag atcgatccga cctgctcggc tcagaacctc gtcgacgacg ctctgcacgg
    13081 atgcatcgtt gatcacgtcg cagatcagca tcgtgatccc atccgcggtg tcgggcatcg
    13141 gctttcggct ggtgccgaag acacggtagc cgtcgcgccg cagcgcttgt gccgtcacca
    13201 gcccgatgcc ggaggaagca cccgtgacca gggcaatgcc gcgttctgtc ttgctcatcg
    13261 gcctctgctt tcattttgtc gtggaacatc gatggacaat cagttactat caaatcaata
    13321 tcaagtcact actaaaaaga tatggacatg aagaatcttt cgagccagcc atgcctgatc
    13381 gcccggagcc tggcgcttgt gggggacgca tggagcatgc tgattatgcg cgacgcccat
    13441 gcggggctga cccggttcga tgatttccgc aagagcctcg gtatcgcgcc gacgatgctg
    13501 acggggcggc tttcagccct gaccgacgag gggctgctgg agaaacgccg ctattccgac
    13561 cgtccgccac gagacgaata tgtgctgacg gaagccggcc gcgacttttt gccggtgctg
    13621 tttgcgatcg gcgcgtgggg acgcaagcat cgcggcgggg gcaaggtgac ccggttcttc
    13681 gacgcagaca ccggaacgga gatcgatccc gtcaccatcg accgcgagac tggcgctccg
    13741 gttggaacac gtcccattcg cgtcgccgca cccgaatgag ccgccccacg gcagatgaat
    13801 gagcgcctga caactcgcat tggacgcccg cttccggcgc aaagttgccg gtagtggtcc
    13861 gtttgtcgag cgatgcctct tggctgttgc tgccaaatgc tgatgcgaga ttaataggtc
    13921 ctgagcccag ggcgggccaa ggcgctattc gcaccgcccg ccatgcatcg cgcgcgcgtt
    13981 cgcgcggccc gttggcttta tctgcacccg atttcagctg ttctcttcgg caaggacctt
    14041 ttgcaccgcg ggccgttccg acatccgttt gcggtgatcc gccaccttcg gcagggtcga
    14101 aagatcgacg ctgtcgcctt ccagccaacc cgccagcgta aacagatagg gatcgcagat
    14161 cgtgtaggtc tcacccatca cccacggccc cttcaacatg ttgcgctcaa tcaacgcgaa
    14221 accggcgccg acgcttttgg ggaccacgtg cttcatgtcg gcaaaggaag cctcatcggt
    14281 cgcccagcga tagccgcgcc ccttgtgggc gtgcgccaca tgcacggtgg agcacagata
    14341 gctgttgaag gcctgcacct gcgcgaatgc gaaagcgtcg tcgaacggcg ccagtttcgc
    14401 ctgcggaaaa ctctgcgcga tgaaagcgag catcgccggc gtctcggtca ggatcccgcg
    14461 atccgttatc agcgccggca ccctcccctt cggattcact gcgagatatt ccggactgtt
    14521 ctgctggttg cttttgaagt cgagcctctc ggtcgtgtag gtggcgcctg cctcctgcag
    14581 ggcgatatga gaagcgagcg cgcagctgcc gggagcgtaa tagagcttga gcatgttggt
    14641 cctcatggca aagccgaaca ataacggcac actctcttgc cgtccatccc aggcggccgg
    14701 gccgtcgcct gcgatgtcag gctttcgcag ttgccgcctg cccgtctcgg gagcggcgtt
    14761 cggctcagcc tatcacgccc gccagaacgg ctcgctgacc tcgtcagcga tcgaggcaca
    14821 gcaggttccc atatcccgca gttcacgatc gctccgcgaa gcgagctctc gccttgactg
    14881 ggcgcgcgcc cgccagattt gaaccgtgga gggaacttcc gaaccaggac gaccagcgaa
    14941 ctgatcgatg ttctcgatgg ctgatgacat tgccggccac tttccgcgta gctgccgctc
    15001 aatgtttggc ccgcgcgatc tttcacgaaa gtcgttcctg acgtttgccc tcgatataaa
    15061 cgctgatgat cgtatcgtcg gcgggctggt gctcgggagc gaccggcgtg agctgggcct
    15121 ggatgcactt ccacgcgccg ttctcgaaca gataggtatc ggtgtacatg gtcataccgg
    15181 tcacttcctg tccgtcgcgg cggcgcgtgt gcttgttggt ggcgcgcacc agcgcaacgt
    15241 cgccgatgac ggtaatgagt tcatcgcgga catcccagta aacgatgatt ttgggatcga
    15301 aggcggtcgc ccaatatttc agataggtcg cgcgatccca tcgctggccg gtgggccaga
    15361 tgttgatgaa ggcgggatga aggatggcgt cgtgcgaggc cacgtcgttg gtcacgaaat
    15421 tatggatgaa gcgcgcattc aacgcacgca attcgttcat cgtctcggcc gatggatgat
    15481 taagctgggc aagctgcatt ttcatctcct gaattttgtc aaagtgccag ttcgcgcgcc
    15541 ttctccgcgg tcgcgctgtc cgccggataa aaacattcga tcttcaattt ttgcagcgcg
    15601 gcgtccatcg gcatcgcaag cgtcgtgaag gtggaaaaga aggcgaggca aaagtcgccc
    15661 tttcttagct gcatcgccat gaccggagac gatccggccg gattgcgcgg aagcctccat
    15721 tcggccggca gcccgggata caccatgatt tcgtcgagca gttgcgccgc caccctgccg
    15781 ccctgtgcga cttcgcgatg caggatctgg atcatctggc cggcgaactc gtcccagttc
    15841 gtgatgaatt gtcgcaggcc ttttggatgg aaaaccacgt gcatggcgtt ttcggccacg
    15901 tcggcttcca tttcgtatga ctggtgaaaa gccttgaaca ggcgtcggga agcctggttg
    15961 cggatgcgga catcccagcg cccatcgatg acaatcccgg gatacggctc ctgctgacga
    16021 agaatgaaat ccagtgcctg gcgtaccggc tgcaaattct cggccgccaa tccccgatcg
    16081 ccgtagggcg gcacgaaacc ggccgcgacg tgcaaggcat tgcactcctc gagcggcaga
    16141 tcgagcgcgc tgccgagcaa ctgcaccatc tcgcggctcg gctgcgcgcg tccggtttcc
    16201 agaaagcaca ggtgcctcgc cgagacattg gcatcgcctg ccagttcgat ctgggtgagg
    16261 cggcgaatgt ttcgccaatg cttcaacaag ctgctgaagt gtcctgtcgc aacatcgtgc
    16321 gtgttcgag
//
LOCUS       DATNOI010000168        13741 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1149459,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000168 DATNOI010000000
VERSION     DATNOI010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13741
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149459"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VN968_06430"
     CDS             complement(<1..227)
                     /locus_tag="VN968_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2852 domain-containing protein"
                     /protein_id="HXL28908.1"
                     /translation="MAYTADVNRWRGPDNERQRPHMLDSPWHPGWIAVTVLGFIIWWP
                     IGLALLFFTLGSRKMGCWNHQDRWANKMERM"
     gene            complement(333..1061)
                     /locus_tag="VN968_06435"
     CDS             complement(333..1061)
                     /locus_tag="VN968_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL28909.1"
                     /translation="MSWRKEQRRAERGYHHGNLKEALLQAALGLIAEKGAAGFTFADA
                     ARMAGVSPAAPYRHFRDRDELLSSIAQRGFEQFEVVLTNAWDDGRPDTVTAFERVGKA
                     YLVFARDEPAFYSAMFESGLPVDLNPTLLAASERAFGIIRAAAERLAAMAPPGTPRPP
                     AMMMALHIWSMSHGVASLFGRGDAARRKLPMSPEDLLEAQVLIYLRGLGFLTDRPAGQ
                     KDAADQKPAGPPPIPPSGPWGTPK"
     gene            complement(1140..1556)
                     /locus_tag="VN968_06440"
     CDS             complement(1140..1556)
                     /locus_tag="VN968_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXL28910.1"
                     /translation="MRFMMLMIPLGYETAPPDVKLDPERVAAMMKYNQALKDAGVLIT
                     LDGLHPPSMGARVSFAGGKPVVTDGPFTEAKEVLGGYWMINVKSREEAIDWAKRCPAT
                     GNEIIEIRQVQELADFPADVQEAAAGLTELQGKPAR"
     gene            complement(1726..2139)
                     /locus_tag="VN968_06445"
     CDS             complement(1726..2139)
                     /locus_tag="VN968_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXL28911.1"
                     /translation="MRFMVIVKADRNSEAGVMPSTELLTAMGKFNEEMVRAGVMLAGE
                     GLHPSSKGARIKFPGKGRTVSRGPFGLTGDLVAGFWLIQVKSLDEAIEWMKRAPFDDG
                     SEIEIRQVFDAEDFGEALTPELREQEQRLRAQATK"
     gene            complement(2365..2733)
                     /locus_tag="VN968_06450"
     CDS             complement(2365..2733)
                     /locus_tag="VN968_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXL28912.1"
                     /translation="MSDDNRTKIIRAIFAAYLSNDRQVVEDSLTDDFRFASPYDDEID
                     KATYFERCWRNSDWIERHELEKIFVEGDEAFVTYKCLAKGGRSFRNTEFFSFEGDKVK
                     RIDVYFGATYENGAFVKQRQ"
     gene            complement(2817..3200)
                     /locus_tag="VN968_06455"
     CDS             complement(2817..3200)
                     /locus_tag="VN968_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL28913.1"
                     /translation="MIVVDVRDMLPEQWRLGVETRMLVSASNGAAQLCIFEQWIAPGA
                     GAATHTHPVEEVLTVREGEAEMWMDERRVIVSAGQSLIVPAERQHGFRNSGNVTLHLH
                     AVLASPIFEATPEGATESVRRWEAV"
     gene            3388..4176
                     /locus_tag="VN968_06460"
     CDS             3388..4176
                     /locus_tag="VN968_06460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL28914.1"
                     /translation="MTPSPQKVALVTGAARGIGLAVAKRFLAEGWRVALLDIEGELLW
                     KSVEALAAAGDTLGLHCDVCDARAVASAIAEIERRFGRLDALVNNAGVAVFAPLLETS
                     DEDWNRVLTVNLTGPFLCTKAAVPLMREHGGGAIVNITSISAVRASTLRSAYGTSKAG
                     LAHLTKQLAVELASLGIRVNGVAPGPVETAMAKAVHTPEIRADYHDAILLNRYGLEEE
                     LAEAVFFLCGDRSSYITGQILAVDGGFDAAGIGLPTLRGDRRNG"
     gene            4377..4673
                     /locus_tag="VN968_06465"
     CDS             4377..4673
                     /locus_tag="VN968_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28915.1"
                     /translation="MSGLLINLIIQIISGAIGGNVAASTAKNIDLGTLGNTIAGAVGG
                     GAGGQILGMLIPLLANSASTPDIGSIIGQVAGGGIAGAVLTAIVGAIKNRTTAA"
     gene            complement(4766..5332)
                     /locus_tag="VN968_06470"
     CDS             complement(4766..5332)
                     /locus_tag="VN968_06470"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXL28916.1"
                     /translation="MSVEANIWLIRHAPVDGPRGVIHGPDAPADVSDAAAFAALRARL
                     PKNAFAVCSPARRAWETAAALGLDPIKEPNFREQDFGAWTGRRHNDLVAELGDAYKDF
                     WKQPASNRLPGGESFVDQISRVRAGLAALPPGDVVLVAHSGTIRAVLAIALDLATDGA
                     LRFVIDPLSLTRIDRLENGWRVVAVNQR"
     gene            complement(5342..6094)
                     /gene="cobS"
                     /locus_tag="VN968_06475"
     CDS             complement(5342..6094)
                     /gene="cobS"
                     /locus_tag="VN968_06475"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HXL28917.1"
                     /translation="MNDWLDDLKTAVAFLTRLPMPHPMGPMAANFARAQRMFPLVGAA
                     IGAVMGLVCLGMRMIGVPDLAAAALALGASAILTGALHEDGLADVADGFGGGRDPAAK
                     LEIMRDSRLGTYGALILMVSFATKLSALAALPDAVVVQGLIAAHALARGVLPAMSMSL
                     PYARKDGLAANAGRPDLATVAMAGVFAFVIALLALPWFDALGAVLVAAVAAIGMAWLA
                     RRQIGGQTGDVLGGAEQVAETAILVTLAAQLA"
     gene            6462..6896
                     /locus_tag="VN968_06480"
     CDS             6462..6896
                     /locus_tag="VN968_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28918.1"
                     /translation="MAITKAVFLAVLLCGLVVPPAQAEVRILASPGGQVGPFLDLFER
                     VRASGERVVIDGPCLSACTLVLSMVPNNRICVTRRAILGFHAARSIDARGHLYAEPEA
                     SELVLEAYPSPVRGWILRRGGLTSRLLLLRGRELTAIYRSCR"
     gene            complement(6901..7317)
                     /locus_tag="VN968_06485"
     CDS             complement(6901..7317)
                     /locus_tag="VN968_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL28919.1"
                     /translation="MQVNPYLYYNGNCEAAFKFYEKVLGAKVEMMLTHESAPESMPTP
                     PEWKKKIMHARISIDGEVIMGSDAPPGHFHQPQGFSVSLLIEDLADAERKFKALSEGG
                     SVNMAFAKTFFAKGFGMCVDQFGIPWMVFCPMPMES"
     gene            complement(7354..7890)
                     /locus_tag="VN968_06490"
     CDS             complement(7354..7890)
                     /locus_tag="VN968_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXL28920.1"
                     /translation="MFEIIAIIAVVFAIVIAIVLILAATKQNTFSVQRATTVKAPPER
                     IFPLINDFHQWGSWSPYENKDPAMKRSYSGAADGKGAVYGWEGNKNVGSGRMEILDTS
                     KSSKIVIKLDFFTPFEGHNTAEFTMLPQGDATNLTWLMHGPAPFMSKVMQVFINLDNM
                     IGKDFEIGLANLKRLTEK"
     gene            complement(7928..8341)
                     /locus_tag="VN968_06495"
     CDS             complement(7928..8341)
                     /locus_tag="VN968_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXL28921.1"
                     /translation="MPTIAGTAPVSKPALWMGRVLSGLVIVFMLFDGAIKLVPWPVVT
                     ETMDRIGYGSSETLARSLGVITLVCTILYAIPPTSILGAILLTGYLGGAIASHLRIGS
                     PLFSHILFGLYLGLMVWGGLWLRDRSLRALMPLQR"
     gene            complement(8383..8826)
                     /locus_tag="VN968_06500"
     CDS             complement(8383..8826)
                     /locus_tag="VN968_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXL28922.1"
                     /translation="MNIQDSKTIDETEIRTLIDQRAKALYAKDAQAVLSCLAADFVAF
                     SLAPPLISAMTDARSYEAWFATWQGPIGTEIRDLAIEVSGDLGVSHSLNLMAGTAASG
                     HEVKLWYRQTLSFRKTDGAWKIVHEHASVPFHMDGSFRAAIDLKP"
     gene            complement(8903..10177)
                     /locus_tag="VN968_06505"
     CDS             complement(8903..10177)
                     /locus_tag="VN968_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXL28923.1"
                     /translation="MTDTAWIDTALTSARPQAVGALLRYFRNLDTAEEAFQNACLRAL
                     KSWPQNGPPRDPAAWLIMVGRNVAIDDIRRSRKQEPLPEDEAISDLDDAEEALAERLD
                     GSHYRDDILRLLFICCHPELPATQQIALALRIVSGLTVKQIARAFLVTEAAMEQRITR
                     AKARVADADVPFETPGAVERAERLSAVAAMIYLIFNEGYSASGDTAEIRSPLCEEAIR
                     LARLLLRLFQSEPEIMGLTALLLLQHARAAARFDAEGAFVLLDDQDRSLWNQKMIAEG
                     LALIDKAMRHRRSGPYQIQAAIAALHARAARPEDTDWTQIDLLYGALEIMQPSPVVTL
                     NRAVAVSKVRGAEAALELIEPLGQRLSSYFHFFGVRGAFLMQLGRNDEARVAFDRAIA
                     LANTSAEAAHIRMHLDRLMRDSQPHGSEISKK"
     gene            complement(10174..10542)
                     /locus_tag="VN968_06510"
     CDS             complement(10174..10542)
                     /locus_tag="VN968_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXL28924.1"
                     /translation="MLYAILCYHDEDTVGSWTKEQDAAVMKKLAVVQGGLTKQGRLGP
                     VARLLPTTSATTLRKDDPPLVIDGPYAETKEQLLGFYIVDCNNLDEALDVARDLGAAN
                     PGGAYEIRPVGMMTPGSLKT"
     gene            10672..11637
                     /locus_tag="VN968_06515"
     CDS             10672..11637
                     /locus_tag="VN968_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HXL28925.1"
                     /translation="MNKAALALIVRGGSANWSPERWKSRFDEVCRGRRVVLLPDAPCD
                     PADIHYAAVWKPGHGELAAFPKLRVIFNLGAGVDALMADSSLPKVPLVRVAVGDLTGR
                     MTEYVVLHVLMHHRQEPYLRACQREKRWEPRSQWAANAISVGVMGLGTLGADAADALR
                     RVGFRVSGWSRNPRRIDGIDCYHGNAQLDTFLQRTDILVCLLPLTPDTRHILNRELFA
                     KLNRNSPLGAPVLINAGRGGLQNEADILACLDDGTLGAASLDVFADEPLPPNSRFWTH
                     PNVVLTPHNAADTDPDEISKYVARQIDRFEADGALENVVDPARGY"
     gene            complement(11719..12378)
                     /locus_tag="VN968_06520"
     CDS             complement(11719..12378)
                     /locus_tag="VN968_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL28926.1"
                     /translation="MVQKNKGPPPRAAPEPAAPKRRGRPRAYQPEIALGKALDLFRKD
                     GFAATSLDDLSAATGMNRPSLYGAFGDKRGLYLKSYARYRADARTAMIDIFRDELPIR
                     ERLDRIYAVALDIYLSGDAGPRGCFTVITAASEAISDPEIRAMVLEGFSELDKAFAAC
                     FRLAKENGELPASADPQVLAQLASATIHTIAIRARARVPRKELEAIVAGAIDVMVPPQ
                     R"
     gene            12477..13121
                     /locus_tag="VN968_06525"
     CDS             12477..13121
                     /locus_tag="VN968_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HXL28927.1"
                     /translation="MDLYFSPLACSLATRIALYETGAEARYLEVDPKTKVVQNDGSDF
                     YQVNPLGLVPTLRTDDGLLLTENAAILQYVADRFPQAGIATGPGMERSRLHQWLCFIG
                     TELHKGLFVPVLDKKAPAEVKTYILGKGLSRLDYLENYLKGREFLLDHFSVADAYLVT
                     VINWTMATPPIELAKWPTVKAYYERLRARPSIAKAVAEEFKLYQAELARHKAAA"
BASE COUNT         2516 a         4369 c         4240 g         2616 t
ORIGIN      
        1 tgcatccgct ccatcttgtt ggcccagcgg tcttgatgat tccagcaacc catttttctg
       61 ctcccgagtg tgaaaaagag aagggcaagc ccgatcggcc accagatgat gaaaccgagc
      121 acggtcacgg cgatccagcc ggggtgccag ggcgaatcaa gcatatgcgg acgctgacgc
      181 tcgttgtcgg ggccgcgcca tcgattgaca tctgcggtgt aggccatttc cctctccatg
      241 acggcatcac gccgttgtga atgtaaataa catttacata cgtaacccat cccggatttt
      301 tgtcaagccg accgcgggcg gcgggctcga atttatttag gggtgcccca gggacctgat
      361 ggcggtatcg gcggcggccc ggccggcttt tgatccgccg cgtctttttg cccggcggga
      421 cggtcggtca ggaaaccgag gccacgcagg tagatcagca cctgcgcctc caggagatcc
      481 tcgggcgaca tcggcagctt gcggcgcgcc gcatcgccgc gtccgaacag cgatgccacg
      541 ccgtgcgaca tcgaccagat atgcagcgcc atcatcatcg ccggcgggcg cggcgtgccg
      601 ggcggcgcca tcgccgcaag gcgctcggcg gcggcccgga tgatgccgaa agcgcgctcg
      661 ctcgccgcca acaaggttgg attaaggtcg acgggaagcc ctgattcgaa catcgccgaa
      721 tagaacgccg gctcgtcgcg ggcgaacacg agataggcct tgccgacccg ctcgaacgcc
      781 gtgacggtat ccggccggcc atcgtcccag gcattggtca gcaccacctc gaactgctcg
      841 aaaccgcgct gcgcaatgct cgaaagcagt tcgtcgcgat cgcggaaatg ccggtacggc
      901 gctgccgggc tgacgcccgc catgcgcgcg gcatcggcaa aggtaaaacc cgccgcgcct
      961 ttttctgcaa tcaggccgag cgccgcctgc agcagcgcct ctttcagatt gccgtgatga
     1021 tagccgcgct cggcgcggcg ttgttccttg cgccagctca tgtgaagggc ttttacatga
     1081 gccgggcgca aaggtcacta gcgcgctcgc tgggccacga ggtattgacc ggccattaac
     1141 tatcgcgccg gcttgccctg caactcggtc agtccggcag cggcttcctg aacgtcggcg
     1201 ggaaaatccg cgagttcctg gacctggcgg atttcgatga tttcgttgcc ggttgccggg
     1261 cagcgcttcg cccagtcgat cgcctcctcc cgcgacttca cgttgatcat ccagtagccg
     1321 ccgagcactt ccttggcctc ggtgaaggga ccgtcggtca cgaccggctt gccgccggcg
     1381 aacgacaccc gcgcgcccat cgacggcggg tgcaggccgt cgagcgtgat cagcacgccg
     1441 gcgtctttca gggcctgatt gtatttcatc atcgccgcca cgcgctcggg atcgagctta
     1501 acgtcgggcg gcgcggtctc gtagcctaag ggaatcatca gcatcatgaa tcgcatggtg
     1561 gagtgtcctg ttacttagtc gccaactttg ccgtcattcc gggatgtgcc ttcggtcggc
     1621 aattgccgac ctgaggcgca ggcccggaat ccatactccc ggtcgtggtt atggattccg
     1681 ggcccgcgct tcgcgcgccc cggaatgacg acggttaaag ccgttctact tcgttgcttg
     1741 cgcgcggaga cgttgctcct gctcgcgcaa ttcgggcgtc agcgcttcgc cgaaatcctc
     1801 ggcgtcgaag acctgccgga tttcaatctc ggagccgtca tcgaaaggcg cccgcttcat
     1861 ccattcgatc gcctcgtcca gtgatttgac ctggatcagc cagaaaccgg caaccagatc
     1921 gccggtaagg ccgaacggac cgcggctcac ggtgcgtccc ttgcctggaa acttaatgcg
     1981 cgcgcccttc gagctcgggt gcagcccctc gcctgcgagc atgacgccgg ccctcaccat
     2041 ctcttcgttg aacttgccca tggccgtcag caattccgtg ctcggcatca cgccggcctc
     2101 gctgttcctg tcggctttca caatcaccat gaatcgcatc atcactctcc gttgatcaac
     2161 cggccggttc gcctcgccga accatggctc gtcaatatga cgaacccggt tttgcggcgc
     2221 cgacatgtca ggcgaaatta tttccgaggt aattgccggc aaaatggcac ggcaagtgcc
     2281 gcctgcccta atcgtcggcc ttgattccac gggaccagat cgcgccgaac cgacatctcg
     2341 atcacacttc tgttgccgct gttgctattg ccgctgtttc acgaacgcgc cattctcgta
     2401 ggtggcgccg aaataaacat cgatgcgttt gaccttgtcg ccttcaaagc tgaaaaactc
     2461 cgtattgcga aaactcctgc cgccttttgc caggcatttg taggtcacaa atgcctcatc
     2521 gccttcgacg aagatctttt ccagttcgtg ccgctcgatc cagtcgctgt tgcgccagca
     2581 gcgctcgaaa taggtcgcct tgtcgatctc gtcgtcatac gggctggcaa aacggaagtc
     2641 atcggtgagg gaatcctcaa cgacttggcg atcgttggat aggtaggcgg caaagatcgc
     2701 acggatgatt tttgtcctgt tgtcgtcgga catggcttag cctcccgtca cttcgccgtt
     2761 tcgtagcgct ggataacaat gccgatcgag agagcaacgc ccgattacag gtctgttcac
     2821 accgcctccc atcgccgcac gctctcggtg gccccctccg gcgtcgcttc gaagatcggc
     2881 gacgccaaga ccgcatggag atgaagcgtg acgttccccg aattgcggaa gccgtgttga
     2941 cgctctgccg gcacgataag cgactggccg gctgagacga tgacgcgccg ctcgtccatc
     3001 cacatttccg cttcaccctc gcgcacggtc agcacttcct cgaccggatg cgtatgggtc
     3061 gcggcgccgg cgccgggcgc gatccattgt tcgaagatac ataattgcgc cgcgccgttg
     3121 ctggccgaga ccagcatgcg ggtttccacg cccagccgcc attgctccgg cagcatatcc
     3181 ctgacatcga ccacgatcat cggactggtc ctttcattcg ccaagacttg tcattcgcca
     3241 acttgcccac ggctcgccgt ttcaagcgtg ccccggaagc tccacgacgc gcaagcggca
     3301 ggaatcatgc cgcgccgcgt cccgcgacac cgcgcacggc atgggctata ggagcgcaaa
     3361 atcgaccaac cgcaaacgga gccatccatg accccctccc cacaaaaagt cgcgctggta
     3421 accggcgccg cgcggggcat cgggcttgcg gtggcaaaac ggtttctcgc cgagggctgg
     3481 cgcgtggcgc tgctcgacat cgagggcgag ttgctatgga aaagcgtcga ggcgctggcc
     3541 gccgccggcg atacgctggg gctgcattgc gacgtctgcg atgccagggc ggtggcaagc
     3601 gcgatcgctg aaatcgaacg gcgtttcggg cggctcgacg cgctggtcaa caatgccggc
     3661 gtcgcggtgt tcgcgccgct gctggagaca tccgacgagg actggaatcg cgtgctgacc
     3721 gtcaatctca ccgggccgtt cctgtgcacc aaggcggccg tacccctgat gcgcgagcac
     3781 ggtggcggcg cgatcgtcaa cattacctcg atctcggcgg tgcgcgcctc gacattgcgc
     3841 tcggcctacg gcaccagcaa ggccgggctg gcgcatctga caaaacagct cgccgtcgaa
     3901 ctggcctcac tcggcattcg ggtcaatggt gtcgcacccg gcccggtcga gaccgcgatg
     3961 gcgaaagccg tccatacgcc ggagattcgc gccgactatc acgacgcgat cctgctcaat
     4021 cgttacggtc tcgaggaaga gctggcggag gcggtgttct tcttgtgcgg cgaccgctcc
     4081 agctacatca ccggacagat tctcgccgtc gatggcggtt ttgatgccgc cggcatcgga
     4141 ttgccgacgc tgcgcggcga cagacgaaac ggctagcatc tcttcccctc tccccgcttg
     4201 cgccgcacca tttctggccg aacctgcatg cgggttgccg aagcattttt ctttccaatc
     4261 cttcgctggc gcagcatgac cttcccggtc caaacgccta agttgtatcg tgtggcgatg
     4321 gttcggcgcc ctgcaagaat ctgaaactga acaccggacc gggggagtat cgcgttatgt
     4381 cgggattact catcaatctg atcatacaaa tcatcagcgg cgccatcggc ggaaatgtcg
     4441 ctgcgagcac ggccaagaat atcgatctcg gtacgcttgg aaacaccatc gcaggtgcgg
     4501 tcggtggcgg cgcaggcgga cagatcctcg gcatgctgat tccgctgctc gccaattcgg
     4561 cctcgacgcc ggatatcggc agcatcatcg gccaggtggc cggtggcggc atcgcgggtg
     4621 ccgttctcac tgcgatcgtc ggtgccatca agaacaggac gaccgcggct tgacggttct
     4681 gcgatcatga acggagcggg gcacgaatcc gtgcctcgct cgatcgcgcc aacgatcgtg
     4741 gatgcgctat ccacccgcgc cccattcaac gctggttgac cgcgaccacc cgccagccgt
     4801 tctcgaggcg atcgatccgc gtcagggaca gcgggtcgat cacaaaacgc agggcgccat
     4861 cggtggcaag atcgagcgcg atggcgagca ccgcgcgaat ggtgccggaa tgcgcgacga
     4921 ggacgacgtc gcccgggggc agcgccgcaa ggcctgcgcg cacacgggaa atctggtcga
     4981 caaaactttc gccgccgggc agccggttgc tcgccggctg cttccaaaag tccttgtagg
     5041 cgtcgccgag ttcggcgacg agatcgttgt gccgccgtcc ggtccaggcc ccgaaatcct
     5101 gttcgcgaaa gttaggctcc ttgatcggat cgagcccgag ggcagcagcg gtttcccacg
     5161 cccggcgcgc cggactgcaa acggcgaaag catttttcgg caatctcgcc ctgagcgcgg
     5221 cgaaggctgc ggcatcgctg acatccgccg gcgcgtccgg accatggatg acgccgcgcg
     5281 gtccgtcgac cggggcgtgc cggatcagcc agatatttgc ctcaacgctc atcgatgaag
     5341 cttatgccag ctgggctgcg agcgtcacga gaatcgcggt ctcggcgacc tgctcggcgc
     5401 cgccgagcac atcgccggtc tggccgccga tttgccgtcg cgccagccac gccatgccga
     5461 tcgcagcaac ggctgccacc aacaccgcgc ccagcgcgtc gaaccacggc aacgccagca
     5521 aggcaataac aaacgcaaag acaccggcca ttgccaccgt cgccaggtct ggcctgccgg
     5581 cattggcggc aagtccgtcc ttgcgcgcgt agggcaggct catcgacatc gccggcaaca
     5641 cgccgcgtgc cagcgcatgc gcggcgatca ggccttgcac gaccaccgcg tccggaagcg
     5701 cggcgagcgc ggaaagcttg gtcgcaaaac tcaccatcag gatcagcgcc ccgtaggtgc
     5761 cgagccggct gtcgcgcatg atctcgagct ttgccgcggg atcgcggccc ccgccaaatc
     5821 cgtcggcgac atcggcgaga ccgtcctcgt gcagcgcgcc ggttaggatc gcgctcgcgc
     5881 ccagggccag tgccgccgcg gcgagatcgg gcacaccgat catgcgcatg ccgagacaga
     5941 ccagtcccat cacggcgccg atcgcggcgc cgacgagcgg aaacatccgc tgggcgcggg
     6001 caaaattcgc tgccatcggc cccatcggat gcggcatcgg cagccgggtc aggaacgcga
     6061 ccgcggtttt gagatcgtcg agccaatcgt tcatgatgca gaacccaagc aattatcttg
     6121 ttcgcgccgc gcccgacacg ggaatcgcat gcggtttttc aggttagacg atattcgtac
     6181 actctgtcgc gatctgcctg ttggtgacgc gcgcgcggct gacgctgcaa ctgtccgcca
     6241 gcagacattg acaagaccgt cgggaagtct cgcgatcacc atcgccatat tttgccggcc
     6301 atcgtggcat gggtgcgcgc ggcgctttcc tgccacactg gcatggctac attaccccgg
     6361 gctggtgttt acagcgccga cgggtacggg accttgtcgc gcatcctgcc attgactatt
     6421 tcaaccgatc aatctgagcg aaggagccgc tcttgatgcg gatggcgatc acgaaggcag
     6481 tgtttctcgc agtgctgctg tgcgggctgg tcgtgccgcc ggcgcaggcc gaggtccgca
     6541 tcctggccag tccaggcgga caggtcggtc cgttcctcga tctgttcgag cgcgtgcgtg
     6601 cttcgggcga acgcgtggtg atcgacgggc cgtgcctgtc ggcctgcacg ctggtgctga
     6661 gcatggtgcc caacaaccgc atctgcgtga cgcgccgcgc gatcctggga tttcacgctg
     6721 cgcggtcgat cgacgcgcgc ggccatctgt acgccgaacc ggaagcgtcc gaactggtgc
     6781 tggaggccta tccctccccg gtgcggggct ggatcctccg ccgcggcgga ctgacgtcgc
     6841 ggctgttgct gttgcgcggg cgagaactca cagcaattta tcgctcgtgc cggtagagcg
     6901 tcagctttcc atcggcatcg gacagaacac catccaggga ataccgaatt gatcgacgca
     6961 catgccgaag cccttggcga agaaagtctt cgcaaatgcc atgttgacgc taccgccctc
     7021 tgacagggcc ttgaacttgc gctcggcgtc agcgaggtcc tcgatcagca atgagaccga
     7081 aaacccctgc ggctggtgga agtggcccgg gggtgcgtcg gatcccatga tcacctcgcc
     7141 atcgatcgat atcctggcat gcatgatttt cttcttccac tccggcgggg tcggcatgga
     7201 ctccggcgcg ctttcgtgcg tcagcatcat ctcgaccttt gcgccaagca ccttctcata
     7261 gaacttgaac gccgcttcgc aattgccgtt gtagtataga tagggattga cctgcatcgc
     7321 ttcctccttg gtgataaagt ttcgaacgcc cggttatttc tcggtgagcc gtttcaggtt
     7381 ggcgagaccg atttcaaagt ccttgccgat catgttgtcc aggttgatga acacctgcat
     7441 caccttggac atgaagggcg ccggaccatg catcagccat gtcagatttg tggcatcgcc
     7501 ctgcggcagc attgtgaact cggcggtgtt gtgaccctcg aacggcgtga aaaaatcgag
     7561 cttgatgaca attttcgaag atttcgaggt gtccaggatt tccatgcggc cggaaccgac
     7621 attcttgttg ccttcccatc catagaccgc gcccttgcca tcggccgcac cgctatagct
     7681 gcgcttcatc gcgggatcct tgttttcata gggcgaccag cttccccatt gatgaaagtc
     7741 gttgatcagc ggaaagattc tttccggcgg cgctttcacc gtggtcgcgc gctgcacgct
     7801 gaacgtattc tgcttggtcg cggcgaggat gagcacgatt gcaatcacga tggcgaacac
     7861 gacggcgatg atggcgatga tctcgaacat aagtcgctcc gtttcgatga attgtcagcg
     7921 agacaaatta gcgttgcaag ggcattaacg cgcgcaggct cctgtcgcgc aaccagagcc
     7981 cgccccacac catcaggccg agatagagcc cgaacaggat gtggctgaac agcgggctgc
     8041 cgatccgcaa atgcgatgcg atcgcgccgc cgagataacc ggtcaggagg atcgcgccga
     8101 ggatcgaggt cggcgggatc gcgtagagaa tggtgcagac cagtgtgatg acaccaaggc
     8161 ttcgcgccag cgtttcgctc gaaccatagc cgatcctgtc catcgtttcc gtgacaacgg
     8221 gccacggcac cagcttgatg gcgccgtcga acagcatgaa cacaataacg agaccgctga
     8281 gaacgcggcc catccagagc gcgggtttcg agaccggagc ggtcccagca atcgtcggca
     8341 tggctctctc cttgagcggt tggttgtcgg gactctctga cgtcacggct tcaaatcgat
     8401 cgcggcgcgg aagctgccgt ccatatggaa aggcaccgag gcgtgctcgt gcacgatctt
     8461 ccaggcaccg tcggtcttgc ggaagctcag ggtctggcgg taccagagct tgacctcgtg
     8521 cccgctcgct gcggtgccgg ccatcagatt gaggctgtgg ctgacgccga gatcgccgct
     8581 cacttcgatc gcgaggtcgc ggatttcagt gccgatcggt ccctgccatg tggcaaacca
     8641 ggcctcgtac gacctcgcat cggtcatcgc cgagatcagc ggcggcgcca gtgagaacgc
     8701 gacgaaatcc gccgcgagac acgatagcac cgcttgggcg tctttcgcat acagtgcctt
     8761 tgcgcgttgg tcgattaagg tacggatttc ggtttcgtcg atagtcttgc tgtcttgaat
     8821 gttcatcgcg cgtttccttc ggtggattgg tgatgcacaa tccaaggacg aacggggtgg
     8881 gcgcgatccg acagaaagcg aattattttt tcgagatttc ggagccgtgc ggctggctgt
     8941 cacgcatcag gcggtcgaga tgcatccgga tgtgggcggc ttccgcggag gtgttggcga
     9001 gcgcgatggc gcggtcgaag gccacgcggg cttcgtcgtt gcggccgagc tgcatcagga
     9061 aggcgccgcg cacgccgaag aaatgaaaat aactcgaaag ccgctgtccc agcggctcga
     9121 tcagttcgag cgccgcttcg gccccgcgca ccttggacac cgccaccgca cggttgagcg
     9181 tcaccaccgg cgacggctgc atgatttcga gcgcgccata aagcagatcg atctgggtcc
     9241 agtcggtgtc ttcgggcctg gcggcgcggg catgcaatgc ggcgatcgcc gcctgaatct
     9301 ggtagggccc gctgcggcga tgccgcatcg ccttgtcgat cagcgcgagg ccctcggcga
     9361 tcatcttctg gttccacagc gagcgatcct gatcatcgag cagcacgaat gcgccctcgg
     9421 cgtcaaagcg cgccgccgca cgggcatgct gcagcaacaa tagcgcggtg agccccatga
     9481 tctcgggctc gctctggaac agccgcagca acagccgcgc caggcgaatg gcttcctcgc
     9541 acagggggct gcggatctcg gcggtgtcgc cgctcgccga atagccctcg ttgaagatca
     9601 ggtagatcat cgcggcgacc gcggagagcc gctcggcgcg ctccacggcg cccggggttt
     9661 cgaacggcac atcggcgtcc gcgacgcggg ccttggcgcg ggtgatgcgc tgctccatcg
     9721 ccgcttccgt aaccaggaac gcgcgcgcga tctgcttcac ggtgagaccc gagacgatcc
     9781 gcagcgccag cgcgatctgc tgcgtcgccg gcagctcggg gtggcagcag atgaacaaga
     9841 gccgcaaaat gtcgtcgcgg tagtgcgagc cgtcgagccg ttcggccagc gcctcctcgg
     9901 cgtcgtcgag atcggagatc gcctcgtcct cgggcagcgg ctcctgcttg cggctgcgcc
     9961 ggatgtcgtc gatcgcgaca ttgcggccga ccatgatcag ccacgcggcg ggatcgcgcg
    10021 gcggcccgtt ctgcggccag cttttcaagg cgcgcaggca ggcgttctga aaggcttcct
    10081 cggcggtgtc gagattgcgg aaataacgca acagcgcgcc gaccgcctgg ggacgcgccg
    10141 aagtcagcgc ggtatcgatc caggcggtgt cggtcacgtc ttgaggctcc ccggtgtcat
    10201 catgccgacg ggacggattt cataggcgcc gccgggattg gcggcgccga gatcgcgcgc
    10261 gacatccagc gcctcgtcga gattgttgca gtcgacgata tagaagccga gcagctgttc
    10321 cttggtttcg gcatagggcc cgtcgatcac cagcggcgga tcgtccttgc gcagcgtggt
    10381 cgccgacgtc gtcggcaaca gccgcgccac cggcccgagc cggccttgct tggtcagtcc
    10441 gccctgcacg acggcaagct tcttcatgac cgcggcgtcc tgttccttgg tccaggaacc
    10501 gacggtgtct tcatcgtggt agcaaaggat ggcgtaaagc atgaggggct cctgttgatg
    10561 ggaggacgaa tgagtatgcc cgtggccgac aggccgggga aatattttgc gcagacactt
    10621 tgggccgata tggtcgcccg cagatcccgc aaaatatgag gctgaaatcc gatgaacaaa
    10681 gcagcgctcg ccctgatcgt aaggggcgga tccgccaact ggtcgcccga acgatggaaa
    10741 agccggttcg acgaggtctg caggggtcgt cgggttgtcc tgttgccgga tgcgccatgc
    10801 gatcctgccg acatccatta cgccgccgtc tggaagccgg gccacggcga gcttgcggca
    10861 tttccaaagc tgcgcgtgat tttcaacctc ggcgcgggcg tcgatgcgct gatggccgat
    10921 tcgagcctgc ccaaagtgcc gcttgttcgc gtggcggtcg gcgatctcac cggccgaatg
    10981 accgaatatg tcgtgcttca cgtgctgatg catcatcggc aggagcctta ccttcgagcc
    11041 tgccagcgcg agaagcgctg ggagccaagg tcgcaatggg cggcgaacgc gatttcggtc
    11101 ggcgtcatgg gccttggcac gcttggcgca gatgccgccg atgcgctgcg gcgagttggc
    11161 tttcgcgtat cgggctggag ccgaaatccc cggcggatcg acggcatcga ctgctatcac
    11221 ggcaacgcgc aacttgatac gtttctgcaa cggaccgata tcctcgtctg tctgctgccg
    11281 ctcacgccgg atacgcgaca catcctcaac cgggaattgt tcgcaaaact caatcgaaat
    11341 agcccgctcg gtgcgcccgt cctgatcaat gccgggcgcg gcgggctgca gaacgaggcc
    11401 gacattttgg catgccttga cgatggcacg ctcggcgccg cctcgctgga tgtcttcgcc
    11461 gatgaaccgc tgccacccaa cagccggttc tggacccatc cgaacgtggt gctgacgccg
    11521 cacaacgccg ccgataccga tcccgacgag atttccaaat acgtggcgcg ccagatcgac
    11581 cgttttgaag ccgacggggc gctcgagaat gtcgtcgatc ccgcgcgcgg atattagcgg
    11641 cacggttcac gagcgagacc tcgcttgtgg ctacccccct ccccaaccct cacccgcaag
    11701 agggagggag cacacccgtt aacgctgcgg cggcaccatc acgtcgatcg cacccgccac
    11761 gatcgcctcc aattccttgc gcggcacgcg tgcgcgcgcc cggatcgcga tggtgtggat
    11821 ggtcgcggac gcaagctgcg ccagtacttg cggatcggcc gatgcgggca gttcgccgtt
    11881 ctctttggca agccggaagc aggccgcgaa cgccttgtcg agttcggaaa atccctccag
    11941 caccatggcg cggatctcgg ggtcggagat cgcttcggag gccgccgtga tcaccgtgaa
    12001 gcagccgcgc gggccggcgt cgccggacag atagatatcg agcgccaccg cgtagatgcg
    12061 gtccagccgt tcgcggatcg gcagttcgtc cctgaaaata tcgatcatcg ccgtacgcgc
    12121 atcggcgcga tagcgtgcgt aactcttgag gtagagcccg cgcttgtcgc cgaacgcgcc
    12181 atagaggctc ggccggttca tgccggtggc ggcgctgaga tcgtcgagcg aggtggcggc
    12241 aaaaccgtcc ttgcggaaca gatcgagcgc cttgccgagc gcgatttcag gctgataggc
    12301 gcgcggccgg ccgcggcgtt ttggcgccgc gggctcgggt gccgcccgtg gcggcggccc
    12361 tttatttttt tgtaccattt cgcaataaat tcgttgacca catttatatt gtgcggaaca
    12421 gtataaaaat caacccccgg ttcgatgcaa ctgaaccgaa agccaaggag gcatccatgg
    12481 acctgtattt ttcgccgctc gcctgttcgc tggcgacccg gatcgcgctg tatgagaccg
    12541 gcgccgaagc cagatatctc gaggtcgatc cgaagacgaa ggtcgtgcag aacgacggct
    12601 ctgatttcta tcaggtgaac ccgctcgggc tggtgccgac tctgcgcacc gacgatgggc
    12661 ttttgctcac cgagaacgcg gcgatcctgc aatatgtcgc cgatcgcttt ccgcaggccg
    12721 gcatcgcaac cggccccggc atggaacgca gccggctgca tcaatggctc tgcttcatcg
    12781 gcaccgaact gcacaagggt ttgttcgtgc cggtgctcga caagaaggcg ccggccgagg
    12841 tgaagaccta catcctcggc aagggcctgt cgcggctcga ttatctggaa aactatctca
    12901 agggccgcga attcctgctc gaccatttca gcgtcgccga cgcttatctg gtgacggtca
    12961 tcaactggac catggcgacg ccgccgatcg aacttgcaaa atggccgacg gtgaaagcct
    13021 attacgagcg cctgcgcgcc cgcccgagca tcgccaaggc ggtcgcggaa gaattcaagc
    13081 tgtaccaggc cgaactcgcg cggcacaagg cggcggcgta aggtttgcgc gaatgcgcgc
    13141 ccgatgacag gctccgcgaa tctccgtcgc ggcgctcgtg caaagaccgt cataccaatt
    13201 gagatcgtca tcacctggct tgacccggtg atccatcttc ttcgaaaaac ttttcgcgaa
    13261 gattgatgga tgctcggatc aagtccgggc atggcgagtt cgtttcgagg gctgaggttg
    13321 aggatcgctt cgcacgcacc aacttcaatt tcaaacagcc aaaacgattt cagacgcagt
    13381 ttcacgccct cgcaacgcaa agcgcccgag tcgttgatga atctttcgcc ccctggaggg
    13441 cgtggggaat gccgggtgcc ggcctcgtgt gcagtttgta ttagtagaac gcacacgagt
    13501 aacaacgagt acaccggaat cgcccggcat tcccgcacgc aatggtttta acagcttatg
    13561 tcgtgctctc cccggtgacc gggctctttt gccaccgtcg cctcgcggat atcaggctcg
    13621 tccgcacccg gtagggccga cttgccatcc gcaaaacttg acgccagcgt cggggcgtca
    13681 ggaccacacg attttgccgt ccgcagcaac atctctcgtc agcgtgccgg tgatcgctca
    13741 c
//
LOCUS       DATNOI010000169         7533 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1497351,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000169 DATNOI010000000
VERSION     DATNOI010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7533
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497351"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..883)
                     /locus_tag="VN968_06530"
     CDS             complement(257..883)
                     /locus_tag="VN968_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXL28928.1"
                     /translation="MTIELHTWNTPNGRKISVALEEMGLPYKVFPVNITKGEQIAPAF
                     LAISPNNKIPAIVDPDGPGGKRVSIFESGAILLYLGERTGKFLPAPLIERIPVLEWLM
                     WQMGGFGPMPGQVHHFIALENETDRAYGLKRYMTETRRLYGVLDRRLADREFVADSLS
                     VADFAILGWAWRHPRHKVELSDFPHVERWYNALMARPGVKRGMEAKLD"
     gene            1053..1616
                     /gene="pyrE"
                     /locus_tag="VN968_06535"
     CDS             1053..1616
                     /gene="pyrE"
                     /locus_tag="VN968_06535"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HXL28929.1"
                     /translation="MSKSASRARLAEIIRKRSFGRGEITLASGRKSDFYFNLKPTMLD
                     PEGASLLAELTFEALKDDNLDYVGGLEMGAVPLAGAIAQLSWIKGHPIAAFFVRKKPK
                     EHGARLAVEGLAKGESLQGKRVVIVEDVTTTGGSALKAVEAVREAGGEVVLVLTMVDR
                     DEGATDTFGQAGLPFRSLYKAGEFLKD"
     gene            1945..2760
                     /locus_tag="VN968_06540"
     CDS             1945..2760
                     /locus_tag="VN968_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HXL28930.1"
                     /translation="MRRRMMLVAAAALVSLVALAPRGASAEGLFDFLFGGAQKQQQRQ
                     TSSPANFFTDPFGLNQQAAPPPRPVASSGSGPAFCVRICDGKYFPLMARGNATPVQMC
                     QAFCPASPTKVFFGSTIDGAFSGNGERYADSENAFAYRKALRRDCTCNGRDPAGLAPI
                     DLTLDTSLRQGDIIATTNGLVAYSGVRAGNGQGAEFTPVASYPGLTADIRARLGEMKV
                     APVAAEMVAGEMPLPEVSRDVALPTAAAPKAASSRARSAKVEAGFASDRALND"
     gene            complement(2965..3396)
                     /locus_tag="VN968_06545"
     CDS             complement(2965..3396)
                     /locus_tag="VN968_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28931.1"
                     /translation="MSTIAFDQFALTRIADFARSLSRLHQASRRQPIDDDAIDREFNA
                     VCLSVWGYTTDDFSDDLFSDADHAWLDSLDEAHARIFAAEQGYDLVDDNGMLTDWWGY
                     CWMILAEKRGLLTPENRAAARAAIEEKYLTAPNVIGVIVGR"
     gene            3577..3870
                     /locus_tag="VN968_06550"
     CDS             3577..3870
                     /locus_tag="VN968_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXL28932.1"
                     /translation="MRAAGEYYVYILASRRHGTLYIGVTNNLRKRLEQHRSGHGSEFV
                     KKYGVHRLVHMEVFASPQDAIAREKQLKEWRRDWKIRLVEEHNLDWSDLSDLI"
     gene            complement(3938..4171)
                     /locus_tag="VN968_06555"
     CDS             complement(3938..4171)
                     /locus_tag="VN968_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28933.1"
                     /translation="MRLTIEILIAAMLLSCITATSAAFAAGGKAGRSADGLSCSFSVP
                     ANAPPAARCAAIRRQCGGKFYANYCGDKMLSAM"
     gene            4385..6907
                     /locus_tag="VN968_06560"
     CDS             4385..6907
                     /locus_tag="VN968_06560"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HXL28934.1"
                     /translation="MPDQSFQGNYPAPGQPVDELALSEIKGAILAKLRLAIGKDAGMA
                     TRHDWYKAAALALRDRIVHHWLTTEKQSYDAGRKRVYYLSLEFLIGRLFTDALNNMGL
                     LPVFEAALGDLGVDLSELRKCEPDAALGNGGLGRLAACFMESMATLAIPAIGYGIRYD
                     FGLFRQIIAQGWQQEYPDEWLGFGNPWEFQRPEVVYHVHFGGNVERFTDAKGRDHAIW
                     HPAETVQAVAYDTPIVGWRGHHVNALRLWSARSPDPLKLDVFNTGDYLGATAEEARAE
                     SICKFLYPNDESPAGRELRLRQEYFFVSASLQDLINRHLASDGQLRGLASKAAVQLND
                     THPSLAVTELMRILCDLHNFRWEEAWKITVATLSYTNHTLLPEALETWPVELFERLLP
                     RHLEIIYRINVAHLALADQRCPGDDDFKASVSLIDERSGRRVRMGQLAFVGSHRINGV
                     SAMHSDLMKETVFHDLNHLYPARITNKTNGITFRRWLMLANPRLTSLLRAACGEAVLD
                     DPSKLELLEARASDSAFQQQFRSVKHHNKIVLARLIGERLNVKVDPAALFDIQIKRIH
                     EYKRQLLNILETVALYHAIRDEPQRDWVPRVKIFAGKAAASYRYAKLIIKLINDVAEV
                     VNNDPVIAGRLKVAFLADYNVSLAEVIIPAADLSEQISTAGMEASGTGNMKLALNGAL
                     TIGTLDGANLEIRDLVGSENIAIFGMEALDVVVRRKQGLDATDVIRRSRHLSRAISAI
                     ESGEFSPDDPTRFESIGHALRHLDHYMVSADFDAYFEAQRGIDARWLVVPAWTRASIL
                     NVARMPWFSSDRTIREYAQDIWNVPVGGPGLQAPGVQREATR"
     gene            7020..>7533
                     /locus_tag="VN968_06565"
     CDS             7020..>7533
                     /locus_tag="VN968_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXL28935.1"
                     /translation="MLAKTQQLFDDATRVTAGDSRWQGWTSDDYWAFVGPFGGATAAT
                     ILRALIDHPQRAGDPLALTVNFCAPIAQGAFDLDVRLVKANRSTQHWCVELTQGGAEV
                     ATLATAVFAERRPSWSHQPAEFPKSPPPEQIQPYPKVAAPWARQYDLRFVEGERVSAI
                     HRKTRRPTRFP"
BASE COUNT         1454 a         2376 c         2305 g         1398 t
ORIGIN      
        1 attgcagccc atccttcgag acggccactt cgtgacctcc tcaggatgag gtctaacttg
       61 ttgaaacaca acaacctcat gctgaggagc gagcgacgcg agcgtctcga agcatgggca
      121 gtaagcgact cacaaatcta aggcgctctg cgcaaagcgc agaagccgag acgcctcgga
      181 tgactggatg ccgtgctggg gcctgtcatc gggctcgccg aaggcgagac ccgatggcgg
      241 ggcatgacgc gtcgggtcaa tccagcttgg cctccatgcc gcgcttgacg ccgggccgcg
      301 ccatcaaggc gttgtaccag cgttcgacat gaggaaaatc tgatagctca accttgtggc
      361 gcgggtgccg ccaggcccaa ccgaggatcg cgaaatcggc gaccgagagg gagtcggcga
      421 caaattcgcg gtcagcgagg cggcgatcca gcacgccgta gagccgtcgc gtctccgtca
      481 tatagcgttt caggccatag gcgcggtccg tttcgttctc cagcgcaatg aaatgatgca
      541 cctgtcccgg catcggccca aaaccgccca tctgccacat cagccattcc agtaccggga
      601 tgcgctcgat cagtggcgcg gggaggaact tgccggtcct ctcgccgaga tagagcagga
      661 tcgcgccgga ttcgaagatg ctgacgcgct tgccgccggg cccgtccgga tcgacgatcg
      721 cgggaatctt gttgttcggg ctgatcgcca gaaacgccgg cgctatctgc tcgcccttgg
      781 tgatattgac cgggaaaacc ttgtacggca gccccatttc ctccagcgcg acgctgatct
      841 tgcggccgtt cggcgtgttc caggtgtgga gttcgatggt catttttgtc atacctcgcg
      901 aaagcgggta tccagtaacc cggaacgtca cggccttaca acagccggcg cggaatatgg
      961 gacacgctgt tgacggcgcc acatcggctc tggaatgtgc agggatccgc cgatagattg
     1021 cagccccgct ccagcccccc gagtgcaacg ccttgtccaa gtcagcctcc cgcgcccgcc
     1081 tcgccgagat catccgcaag cgctcgttcg gccgcggtga aatcacgctg gcctcgggcc
     1141 gcaaaagcga tttctatttc aatctgaagc cgaccatgct cgatcccgaa ggcgcctcat
     1201 tgcttgcgga actgacgttc gaggccttga aagacgacaa cctcgattat gtcggcggac
     1261 tggaaatggg cgcggtgccg ctcgccggcg cgatcgcgca gctgtcctgg atcaagggcc
     1321 atccgatcgc agccttcttc gtgcgcaaga aaccgaagga gcatggcgcc cggctcgcgg
     1381 tcgaaggact ggcaaagggc gagagcctgc agggaaagcg cgtggtgatt gtcgaggacg
     1441 tcaccaccac cggcggctcg gcgctgaagg cggtcgaggc ggtgcgcgag gccggcggcg
     1501 aggtcgtgct ggtgctcacc atggtggacc gcgacgaagg tgctaccgac acatttggac
     1561 aggcgggcct tccgtttcgc tcgctctaca aggccggtga attcctgaag gattgatcgt
     1621 ttcttcctgg cacaattgcg gggaaggccg ggactcgtgt cccggacacg gtgcagcgtt
     1681 tttcacgctg ctagccggga cccacacttg cattgcgcag catgggcccc ggatcagcag
     1741 cgcaccgcaa gagcgctgcg cagcatccgg ggcacgtcgc tctgaagcga tgcgctgcat
     1801 ttcttttcgg ccatccggtg cgtgctcata tctcgtttac catctcgcac taatgtgaag
     1861 cggtgctgag gtactacttg ccacaagcgg gttcggttgc atcgggtgga gtaggcgttg
     1921 cgtacagagt ttccttatca gcccatgcgg cgccgcatga tgcttgtcgc cgccgctgcg
     1981 ctcgtaagcc tggttgccct tgcgccgcgc ggcgcgtccg ccgaaggact gttcgatttc
     2041 ctgttcggcg gcgcccagaa acagcaacag cgccagacct cgtcgccagc gaatttcttc
     2101 accgatccgt tcggtctcaa ccagcaggca gcacccccgc cgcgcccggt cgcctcaagc
     2161 ggctccggcc ccgcattttg cgtgcggatc tgcgacggca aatattttcc gctgatggcg
     2221 cgcggcaacg cgacgccggt tcagatgtgc caggcgtttt gtccggccag cccgacgaaa
     2281 gtcttcttcg gcagcaccat cgatggcgcg ttctccggca atggcgagcg ctacgccgac
     2341 agcgaaaacg ctttcgccta tcgcaaggcg ttgcgcaggg actgcacctg taacggccgc
     2401 gatcccgcag gcctcgcccc gatcgatttg acgctcgaca cctcgctgcg ccagggcgac
     2461 atcatcgcca ccaccaacgg gctggtggcg tattcgggtg tcagggccgg caacggccag
     2521 ggcgctgaat tcaccccggt cgcgtcctat ccgggcttga ccgccgatat ccgtgcgcgg
     2581 ctcggcgaga tgaaggtggc gccggtggcc gcggaaatgg tcgcgggtga aatgccgttg
     2641 cctgaggtga gccgcgacgt cgcgctgccc acggccgcgg cgccgaaggc cgcgtcatcc
     2701 agagcgcgat cagcaaaagt ggaagccggt tttgcgtccg atcgcgctct taacgattag
     2761 tgcgcatgat cttttcgcca aaccgctcac actttggcgg atcatgcgcg gcgaagcgag
     2821 cggaagtgaa ttaaacgtaa atcgggatcc tcatggtgag gagcgcgact tcgcgcgtct
     2881 cgaaccatga ggccacagct ggagcctgca gccatccttc gagacggcca cttctcatcc
     2941 tctcaggatg agggccgcag ataattaacg cccgacgatg acgccgatca cgttcggcgc
     3001 ggtcagatat ttttcctcga tggcagcgcg cgccgccgcg cggttttccg gcgtcagcag
     3061 gccgcgcttc tcggcgagga tcatccagca atagccccac cagtccgtca gcatgccgtt
     3121 gtcgtcgaca aggtcatagc cctgctcggc ggcgaagatg cgcgcatgcg cctcgtcgag
     3181 cgaatcgagc caggcgtggt cagcgtcgga aaacagatcg tcgctgaaat cgtccgtggt
     3241 gtagccccac accgagagac agacggcgtt gaactcgcgg tcgatcgcgt cgtcgtcgat
     3301 cggctgccgg cgggaagcct gatgcagccg cgacagcgag cgggcgaagt cagcgatgcg
     3361 ggtgagcgcg aattgatcga aggcgatggt ggacatgtag tcctcgaaat ctcggataga
     3421 ccctagatat tgtgtggacg tggtatcgaa tcactatgat atatcaaagg cttgctaaag
     3481 tgttcttaat tcgttccatt gtcattcatt atcgtcccgg cgaaggccgg gacccataac
     3541 caccgtgtca gagttcttgc gaagtcatgc caaaccatgc gcgcagcagg cgaatattac
     3601 gtctacattc tcgccagccg cagacacgga acgctttaca tcggggtcac caacaacctt
     3661 cggaaacggc ttgagcagca tcgaagcggt catggctcgg aattcgtgaa gaaatacggt
     3721 gtgcatcgcc tcgtccacat ggaagttttc gcatcgccgc aagatgcgat cgcacgcgag
     3781 aagcaattga aggaatggcg acgcgactgg aagattcgtt tggtcgaaga gcataatctg
     3841 gattggagtg acctgtccga tctcatttaa tgccggtggt tatgggtccc ggccttcgcc
     3901 gggacgacga caaccagacc atgacgcttt tcacagctca catcgccgac agcatcttgt
     3961 ctccgcagta attggcgtag aatttcccgc cgcattgccg gcggatcgcg gcgcagcgcg
     4021 cggcgggtgg ggcatttgcc ggaacgctga atgagcaact caggccatcg gcactccggc
     4081 ccgccttgcc ccctgcggcg aatgccgcgc tggtggcggt gatgcaggac agcagcattg
     4141 ctgcgatgag aatctcgatc gtcaatctca tggcccgctc cttcccggaa tgatcgtgac
     4201 accgcatgtt ttgcggattt tacactatca cccaggcgcg cgcatgggaa tttgcgacaa
     4261 atcggcccag tccttgcata aaaaaaccgt gcagcgctgc aaaaacgaag ccccagagcg
     4321 ggtttcgatc caggcccaac gcgggtattg tttggcagag cctcaaatgt ccaggatcca
     4381 agatttgcca gaccaatctt tccagggaaa ttacccggcg cccggccaac ccgtcgacga
     4441 actggcgctg tccgagatca agggcgcgat cctcgccaag ctgcggctgg cgatcggcaa
     4501 ggacgccggc atggccacgc ggcacgactg gtacaaggcg gcggcgcttg cgctgcgcga
     4561 ccgcatcgtg caccactggc tgaccacgga aaagcagagc tacgacgccg gccgcaagcg
     4621 ggtctattat ctcagcctgg aattcctgat cggccgcctg ttcaccgacg cactgaacaa
     4681 catgggcttg ctgccggtat tcgaggccgc gctcggcgat ctcggtgtcg atctctccga
     4741 gcttcgcaaa tgcgagccgg atgcagcact cgggaacggc ggtcttggac ggcttgcggc
     4801 gtgcttcatg gaaagcatgg cgacgctggc tattcccgcc atcggctacg gcattcgcta
     4861 cgatttcggc ctgtttcgcc agatcatcgc gcagggctgg cagcaggaat atcccgacga
     4921 atggctgggc ttcggcaatc cctgggaatt ccagcggccc gaggtggtct accacgtgca
     4981 tttcggcggc aacgttgaac ggttcaccga cgcgaagggc cgcgaccatg cgatctggca
     5041 tccggcggaa accgtgcagg cggtcgccta cgacaccccg atcgtgggtt ggcgcggcca
     5101 ccacgtcaat gcgctgcggc tgtggtcggc gcgctcgccc gacccgctca agctcgacgt
     5161 gttcaacacc ggcgactatc tcggcgccac cgccgaggag gcgcgcgcgg aatcgatctg
     5221 caagttcctc tatccgaatg acgagagccc ggcgggccgc gagttgcggc tgcgtcagga
     5281 atatttcttc gtctccgcct cgctgcagga tctgatcaac cgccatctcg cgtcggacgg
     5341 acagttgcgc ggtctggcgt caaaggccgc cgtgcagctc aacgacaccc atcccagcct
     5401 ggccgtcacc gaactgatgc gcattctctg cgatcttcac aacttccgct gggaggaggc
     5461 atggaagatc accgtggcga cgctgtccta caccaaccat acgctgctgc cggaagcgct
     5521 cgaaacctgg ccggtcgaac tgttcgagcg cctgttgccg cgccatctgg aaatcatcta
     5581 ccgcatcaat gtcgcgcatc ttgcgcttgc cgaccagcgc tgtcccggcg acgacgattt
     5641 caaggcctcg gtatccctga tcgacgagag gtccggccgc agggtacgca tggggcaatt
     5701 ggcgttcgtc ggatcgcatc gcatcaacgg cgtctcggcg atgcattccg atctgatgaa
     5761 ggagaccgtg ttccacgatc tcaatcatct ctatccggcc cgcatcacca acaagaccaa
     5821 cggcatcacc ttccgccgct ggctgatgct ggccaacccc aggctgacca gtctgttgcg
     5881 cgcggcctgc ggcgaggccg tgctcgacga tcccagcaaa ctcgaacttc tcgaggcccg
     5941 cgccagcgat agcgccttcc agcagcaatt ccgcagtgtc aagcaccaca acaagatcgt
     6001 gctggcgcgg ctgatcggcg aacgcctcaa cgtcaaggtc gatcccgcgg cattattcga
     6061 catccagatc aagcgcattc acgaatacaa gcggcaactg ctcaacatcc ttgaaaccgt
     6121 ggcgctgtat cacgcgatca gggacgagcc gcagcgcgac tgggtgccgc gcgtgaagat
     6181 ctttgccggc aaggcggcgg cgagctatcg ctatgccaag ctgatcatca agctcatcaa
     6241 cgacgtcgcc gaagtcgtca acaacgaccc cgtgatcgcc ggacggctga aggtcgcgtt
     6301 cctcgccgac tacaatgtca gccttgccga ggtgatcatt cccgccgccg acctctcgga
     6361 acagatttct accgcgggca tggaggcctc cggcaccggc aacatgaagc tcgcgctgaa
     6421 cggcgcgttg acgatcggca cgctcgacgg cgccaatctc gagatccgcg atctcgtcgg
     6481 ctcggaaaac atcgcgatct tcggcatgga agcgctcgac gtggtggtgc ggcgcaagca
     6541 gggtctcgac gccaccgatg tgatcaggcg atcgcggcat ctttcgcgcg ccatcagcgc
     6601 gatcgagagc ggcgaattct cgcccgacga cccgacccgg ttcgaatcga tcgggcacgc
     6661 gctgcgccat ctcgaccact acatggtgag cgcggatttc gacgcttatt tcgaagccca
     6721 gcgcggcatc gacgcgcgct ggctggtggt gccggcatgg acgcgggcca gcatcctcaa
     6781 tgtcgcgcgg atgccgtggt tctcgtccga ccgcaccatc cgcgaatacg cccaggatat
     6841 ctggaacgtg ccggtaggcg ggcccgggct gcaggcgccg ggcgttcagc gcgaggcgac
     6901 gcgctgacgc gaccggcgct tatccccgat taaaccgatc aaacaagaac cattacctcc
     6961 cacgtcattg aactcgcccg cgtggcgatg atactgcccg agaggatcgg gggaatttca
     7021 tgcttgccaa gacccagcaa ctgttcgacg acgcgacgcg ggttaccgcc ggtgacagcc
     7081 gctggcaggg gtggaccagc gacgattact gggcttttgt cggcccgttc ggcggggcga
     7141 ccgccgccac gatcctgcgg gcgctgatcg accatccgca gcgggccggc gatccgctgg
     7201 cccttaccgt gaatttttgc gcgccgatcg cgcaaggcgc cttcgatctc gatgtccgcc
     7261 tggtcaaagc caacaggtcg acgcagcatt ggtgtgtcga actgacccaa ggcggtgctg
     7321 aagttgcgac ccttgcaacc gccgtgtttg ccgagcgccg cccgtcgtgg tcgcaccagc
     7381 cggcggaatt tccgaaaagt ccaccgcccg aacagataca gccttatccg aaggtggcgg
     7441 cgccgtgggc caggcaatat gatcttcgct ttgtcgaggg tgagcgagtt tcggcaatcc
     7501 atcgaaagac gcgccgccca acgcgttttc caa
//
LOCUS       DATNOI010000170         6318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_758902,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000170 DATNOI010000000
VERSION     DATNOI010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758902"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VN968_06570"
     CDS             <1..670
                     /locus_tag="VN968_06570"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HXL28936.1"
                     /translation="GDAMNFDMAREMSGKSVATVTTDDDVAVETSTFSTGRRGVAGTL
                     IVEKILGAAAEEGRDLAALKVLGDRVNAATRSIGIALTSCTVPAAGKPTFDIAEDEME
                     VGVGIHGEPGRRRVKLRPASEIATEMVNAITADLGERARGEAILLINGFGATPLSELY
                     LMYDAVRRLLEPAGTIVVRSLVGNFVTSLDMAGCSATVSMLDEEMKRHWDAPVHTAAL
                     RWGM"
     gene            690..782
                     /locus_tag="VN968_06575"
                     /pseudo
     CDS             690..782
                     /locus_tag="VN968_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010967853.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
     gene            874..1530
                     /locus_tag="VN968_06580"
     CDS             874..1530
                     /locus_tag="VN968_06580"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXL28937.1"
                     /translation="MTQPDLIIFDCDGVLVDSEVLSCRCLSKVLAGYGIDLGLDQALD
                     LFLGRSMTAVVEYYEALGRSIPEQFSTELTAGVRAAFTSALCPIEGVSSVLEGLRIPY
                     CVASSSDVDRVSLSLSLTGLAPHFDNRLYTSQMVERGKPAPDLFLYAAERMQADPRCT
                     LVIEDSISGVRAAKAAGMTVWGFVGGSHYRSRDGKAILRAAGADRVFGRMADFWRADR
                     "
     gene            1537..2493
                     /locus_tag="VN968_06585"
     CDS             1537..2493
                     /locus_tag="VN968_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HXL28938.1"
                     /translation="MAASDNDKSRLDDAARAGWLYFIAGHTQDEIARMLQVSRASAQR
                     LVSLCLAERLITFRLEHPIAACMELAARLKDLFHLVYCEVVPTDPAAPLSAAGIAERA
                     ANVLESTLRSEKPTIVALGTGRAVRAAVERVSPIDCPNHQIVSLVGNISADGSASFFD
                     TVGRLADRTRARHYPMPLPFLMSSAGERAQMLKIDPIAKVRAVAAKADLRLVGIGQMD
                     QRAQVHIDGFVSREELFEMMRLGAVGELTGWAFDAKGRIIDGGTNQRLTSIPPQVPAK
                     ALTIGAAVGPAKVPAIAAALKGRLINGLVTDETTASAILKQQ"
     gene            2690..4003
                     /locus_tag="VN968_06590"
     CDS             2690..4003
                     /locus_tag="VN968_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HXL28939.1"
                     /translation="MKHVLGALLGVVTLLGSAPSIAQTTLTIATVNNGDMIRMQALTN
                     EFTAKNPDVSVKWVTLEENVLRQRVTTDIATKGGQFDVLTIGTYEVPIWAKKNWLIPL
                     DNLGSDYDTADLLPKIRDAVSVSGKLYAAPFYGESSMMMYRTDLFQKAGLTMPEKPTW
                     DFVVDAAKKLADKSTGVYGICLRGKAGWGENMAFLTAMSNSFGARWFDEKWQPQFDKP
                     EWKKTLTVYVDLMKAAGPPGASSNGFNENLALFNSGKCAMWIDATVAASFVTNPKDSQ
                     VADKVGFALAPNAGLGKNANWLWAWNLAIPAGSKKVAAAEKFIAWATSKDYTKLVASK
                     EGWANVPPGTRTSLYQNAEYLKVAPFAKLTLASIDAADPNKPTVQPVPYVGVQYAAIP
                     EFQGIGTSVGQQFSAALSGSSTVDAALAAAQSATEREMKRAGYIK"
     gene            4120..4992
                     /locus_tag="VN968_06595"
     CDS             4120..4992
                     /locus_tag="VN968_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL28940.1"
                     /translation="MATQQTQVLGRALLTPAVALLFIWMIVPLAMTIYFSTLHYSLLD
                     PGSESFVGLENFRYFLTDPAFLTSLQNTLVLVGSVLAITILLGTPLALLLDQPVIGRS
                     IVRLMVIAPFFVMPTVSALVWKNLLMHPVSGLFAWGATRLGLTPIDWFTDAPLFAVIL
                     IVAWEWLPFATLILLTALQSQDEEQKEAAEMDGASALSTFIYLTLPHLARPITVVILI
                     ETIFLLTVFAEIFVTTGGGPGLQTTNIAFLIYSQALIQFDVGNASAGGLVAVVIANIV
                     AFFLVRIIGRNLEA"
     gene            4996..5826
                     /locus_tag="VN968_06600"
     CDS             4996..5826
                     /locus_tag="VN968_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL28941.1"
                     /translation="MARKVTARRVWVSTAAAWLFGFLIFLPILWMVLTSFKTELEAFA
                     MPPTFLFFHWTTENYATVQGRSDYLHHALNSIMVAGGSTLIALLIAIPAAWSMAFAPT
                     KRTKDVLLWMLSTKMMPPVGVLVPIYLIYRDFGLLDTRAGLVLILCLGNLPIVIWMLF
                     TYFKEIPKDILEAARMDGATIGRELIYVLTPMAIPGLASTLLLNFILAWNEAFWTLNL
                     STLDAAPLTVFIASYSSPEGLFWAKLSAASTLAIAPIIILGWFTQRQLVRGLTFGAVK
                     "
     gene            5849..>6318
                     /locus_tag="VN968_06605"
     CDS             5849..>6318
                     /locus_tag="VN968_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28942.1"
                     /translation="MGQITLQRVRKSFGPVNIIKDANLDIEDGSFVVFVGPSGCGKTT
                     LLRLIAGLEDVTGGQILIDGRNVVDVPPAKRGLSMVFQSYALYPHMSVRGNIAFGLKM
                     AGLPRPEIDRKVEAAAATLNLTPYLDRKPRELSGGQRQRVAIGRAIVREPKAFL"
BASE COUNT         1140 a         1911 c         1975 g         1292 t
ORIGIN      
        1 gggcgacgcc atgaacttcg acatggcgcg cgagatgtcg ggaaaatcgg ttgcgaccgt
       61 gaccaccgat gacgatgtgg cggtggagac ctcgaccttt tcgacgggac ggcgcggcgt
      121 tgccggcacg ctgatcgtgg agaaaatcct gggcgctgcc gcagaagagg gacgcgacct
      181 agccgctttg aaagtgctgg gcgatcgcgt caatgcggcg acgcgctcga tcgggatcgc
      241 gctgacgagc tgcacggttc cggcggccgg caagccgacc ttcgatatcg ccgaggacga
      301 gatggaggtc ggtgtgggca tccacggcga gccgggacgc cgccgggtaa aactcaggcc
      361 ggcttccgag atcgcgacag agatggtcaa cgccatcact gccgatctgg gcgagcgcgc
      421 gcgcggcgag gctatcctgt tgatcaacgg cttcggggca acgccgctct cggagcttta
      481 tctgatgtat gatgcggtcc gccgattgct cgaaccggcc ggcaccatcg ttgtgcgatc
      541 gctcgtcggc aatttcgtca catcgctcga tatggccggt tgctcagcca cggtatcgat
      601 gcttgacgaa gagatgaagc gtcactggga cgcacccgtc cacaccgcgg ccttgcgctg
      661 gggcatgtag tcgtcggagc ggccgacctt catccttcat cgcctttttc gtctcgccgc
      721 gcgctgcctc catcaccggc gcggagtacg tcatcgacgg cggcaccgtt ccgaccgtct
      781 agccggattg gtcgttcaac cccatttgag tctattgtat gggttgttgc ccatgtcgtg
      841 agcatatgct ctgattgggt acggaaacct gcgatgaccc aacccgacct cattattttc
      901 gactgtgacg gcgtcctggt cgacagcgag gtgctgagct gccgttgcct ttccaaggtt
      961 ttggcgggct acggcatcga tctcggtctg gatcaggcgc tcgatctgtt cctcggacga
     1021 agcatgactg cggtcgtgga gtactacgaa gcgctgggac gctcgattcc ggagcaattc
     1081 tcaactgaac tgacggccgg ggttcgtgcc gcgttcactt ccgcgctttg cccgattgaa
     1141 ggcgttagct ccgtgctgga aggcttgcgg atcccgtact gcgtcgcttc gtccagcgat
     1201 gtcgatcggg tgtccctctc gctttcgctg accggactgg caccgcattt cgacaaccgg
     1261 ctctacacct cgcaaatggt ggaacgcggc aaacccgcgc ccgacctctt cctttacgcg
     1321 gccgaaagga tgcaggccga cccgcgttgt acgctggtga tcgaggacag catcagtggc
     1381 gtgagggcgg ccaaagcggc cggcatgacg gtttggggat ttgtcggagg cagccactat
     1441 cgatcgcgtg acggtaaagc gattcttcgt gcggcgggag ccgatcgtgt gttcgggcga
     1501 atggcggatt tttggcgagc ggatcggtag gatttgatgg cagcatccga caacgataaa
     1561 tcgcgcctgg acgacgcagc gcgggccggc tggctctatt tcatcgccgg tcacacccag
     1621 gacgaaatcg ccaggatgct ccaggtttcg cgcgcctccg cccagcgcct ggtttcattg
     1681 tgcctcgccg agcgcttgat caccttccgg cttgaacatc cgatcgcggc gtgcatggag
     1741 ttagcggcgc gtctgaagga cttgtttcac ctcgtctatt gcgaagtggt gccgaccgat
     1801 cccgccgcgc ctttgtcagc tgccggaatt gccgagcgcg ccgcgaacgt cttggaatcc
     1861 acgttgcgca gcgagaagcc cacgatcgtc gcgctgggca ccgggagagc ggtgcgggcc
     1921 gcggtcgagc gtgtctcgcc gatcgattgc cccaatcacc agatcgtgtc gctggttgga
     1981 aacatctcgg ccgacggctc ggcgagcttc ttcgataccg tcgggcggct ggccgaccga
     2041 accagggcgc ggcactatcc gatgccgctg ccgtttctga tgtcgtccgc gggcgagcgc
     2101 gcccagatgc tcaagatcga tccgattgcc aaggtgaggg cggtcgcagc caaggccgac
     2161 ttgcggctgg tcgggatcgg ccaaatggac cagcgcgcgc aggtgcatat tgatggcttc
     2221 gtctcgcgcg aagaactgtt cgaaatgatg cgattgggcg ccgtcggcga gttgaccggc
     2281 tgggcattcg acgccaaggg ccgcatcatc gatggcggga cgaaccagcg cctgaccagc
     2341 attccgccgc aggtcccggc taaagccctg accatcggag cggccgtcgg tcctgccaag
     2401 gtgccggcga tcgcggcggc gctgaaggga cgtctgatca acggcttggt caccgacgag
     2461 acgacggcaa gcgccatcct gaaacagcaa taaatcgatg caaacgctga cggctccgat
     2521 ccagcggtgg gggctggcgt cggcgttcgt gaccgtttcc ggcccgcctt tgctgatggc
     2581 aacgcggtgc atgccgccga tctacgcctt gacaacactg cggctcggtg tgaacataat
     2641 ttcaaggcgt gagcatatgc tcaacgccaa aagacttagg gaggttaccg tgaaacacgt
     2701 cctcggcgcc ctcttgggcg tggttaccct gttaggctcg gccccctcga ttgcacagac
     2761 cacgctgacg atcgccaccg tgaacaacgg cgacatgatc cgcatgcagg cgttgaccaa
     2821 cgagttcacc gccaaaaatc ccgacgtgag cgtgaaatgg gtgacgctgg aagagaacgt
     2881 cctgcgccag cgcgtcacca ccgatatcgc gaccaagggt ggtcagttcg acgtgctgac
     2941 catcggtacc tacgaggtcc cgatctgggc caagaagaat tggctcattc cactcgataa
     3001 tctcggatcc gattacgata cagccgatct gcttcccaag atccgcgatg ccgtatcggt
     3061 gtccggcaaa ctctatgccg cgccgtttta cggcgagagt tcgatgatga tgtatcgcac
     3121 cgaccttttc caaaaggccg gattgacgat gccggaaaag ccgacctggg acttcgtggt
     3181 cgatgcggcg aagaaactcg ccgacaagtc cacgggcgtc tacggcatct gcctgagggg
     3241 caaggccggc tggggtgaaa acatggcctt cctgacggcg atgtccaatt cgttcggcgc
     3301 gcgctggttc gatgagaaat ggcagccgca gttcgacaaa ccggaatgga agaagacgct
     3361 tacggtctat gtcgatctga tgaaggctgc cggccctccc ggtgcaagct cgaacggctt
     3421 caatgaaaat ctggcgctgt tcaactccgg caagtgcgcg atgtggatcg atgctaccgt
     3481 tgcggcctcc ttcgtgacca acccgaagga ttcgcaggtc gccgacaagg tcggcttcgc
     3541 gctggcgccg aatgcggggc tgggaaagaa cgccaactgg ctctgggctt ggaatctcgc
     3601 cattcccgca ggctcgaaga aggttgccgc cgccgaaaaa ttcatcgcgt gggcaaccag
     3661 caaggactac accaagctgg tcgcctcgaa agagggatgg gccaacgttc cgcccggcac
     3721 gcggacttcg ctctatcaaa atgcggaata tttgaaggtc gccccgttcg cgaagctgac
     3781 gctggcttcg atcgacgcgg ccgatccgaa caagccgacg gtgcagccgg tgccttatgt
     3841 tggggtgcaa tacgcggcaa tcccggaatt ccagggtatc ggcaccagcg tgggccagca
     3901 attttcggcg gcactatccg ggtcatcgac cgtcgacgcg gcgctcgccg ccgcgcaatc
     3961 ggcgaccgag cgcgaaatga agcgcgccgg ctacatcaag tagctcgtcg cgtcgatcct
     4021 tgcggcgatt ctccctgtga gccgcaaact ggctgccgtc cgcccgatac tgcccggacg
     4081 gcagcctcat cgagtttccc gaaataagag gaggcggcgg tggcgaccca gcagacacag
     4141 gttctcggac gggccctgct gactccggct gtggcattgc tcttcatctg gatgatcgtc
     4201 ccgctggcga tgacgatcta cttttcaacg cttcactaca gcctgctgga tccgggtagc
     4261 gagagcttcg tcgggctgga gaattttcga tattttctga ccgaccctgc ctttctgacc
     4321 tcgctgcaaa ataccctggt gctggtcggt tcggtcctcg ccatcaccat ccttctcggc
     4381 acgccgctgg cgctgctgct cgaccagccc gtgatcgggc gcagcatcgt gcgactgatg
     4441 gtgatcgcgc cgtttttcgt gatgccgacc gtcagtgcgc tggtctggaa aaacctgctg
     4501 atgcatccgg tctccggact gtttgcctgg ggcgcaacgc gattgggctt gaccccgatc
     4561 gactggttca ccgacgcgcc cttgttcgcg gtgatcctga tcgtcgcctg ggagtggctg
     4621 ccctttgcga ccctcatcct gctcacggcg ctgcaatccc aggacgagga acagaaggaa
     4681 gcggccgaga tggacggggc gagtgcgctg tcgacgttca tctatctcac tctgcctcac
     4741 ctcgcgcggc cgatcacggt ggtcatcctg atcgaaacga ttttcctgct gacggtgttc
     4801 gccgagatct tcgtcacgac cggcggcggc ccgggcctgc agaccaccaa tatcgcgttc
     4861 ctgatctact cgcaggcgct gatccagttc gacgtcggaa acgcttcggc cggcggattg
     4921 gttgcggtcg tgatcgccaa tatcgtggcc ttcttcctgg tgcggatcat cgggcggaat
     4981 ctggaggcgt aagcaatggc acgcaaggta actgcgagac gcgtctgggt gtccaccgct
     5041 gcggcctggt tgttcggatt tttgatcttc ctgccgatcc tgtggatggt gctgacaagc
     5101 ttcaagaccg agctcgaggc attcgccatg ccgccgacgt tcctgttttt ccattggacc
     5161 acggagaatt atgcgacggt gcagggccgc agcgactacc tgcaccatgc gctcaattca
     5221 atcatggttg cgggcggatc gacgctgatc gccctgctca tcgctattcc ggcggcatgg
     5281 tcgatggcgt ttgcgccgac caagcggacc aaggatgttc tgctctggat gctctccacc
     5341 aagatgatgc cgccggtcgg cgtgctggtt ccgatctatt tgatctaccg agattttggc
     5401 ttgctggaca cgcgcgcggg gctggtcctg atcctctgcc tcggcaatct gccgatcgtg
     5461 atctggatgc tgttcaccta tttcaaggaa atcccgaagg acatcctgga agcggcgcga
     5521 atggatgggg cgaccatcgg ccgcgagctc atctatgtgc tgacgccgat ggccattccc
     5581 ggccttgcct cgacgctgct tctcaatttc atcctggcgt ggaatgaggc attctggacc
     5641 ctgaaccttt cgacgttgga tgcagcgccg ctcacggtgt tcatcgcgtc ctattcaagt
     5701 ccagagggcc tgttctgggc aaaactgtcg gcggcttcga cgctcgccat tgctccaatc
     5761 atcatactcg gatggttcac ccagcggcag ctcgtccgcg ggctgacgtt cggcgccgtc
     5821 aagtagatca gcgaaggggt gatagaccat gggccagata acgctccagc gagtccgcaa
     5881 gtcgttcggg ccggtcaata tcatcaagga tgctaacctg gatatcgagg acggctcctt
     5941 cgtggttttc gtcgggcctt caggttgcgg caagaccacg ctgttgcgcc tgatcgccgg
     6001 acttgaggac gtcaccggcg ggcaaatcct gatcgacggc cgcaatgtgg tggacgtgcc
     6061 gccggccaag cgcggtttgt cgatggtgtt tcagtcttat gcgctctatc cgcatatgag
     6121 cgtccgcggc aacatcgcat tcggcctgaa gatggcgggt ctgccgcgcc cggagatcga
     6181 ccgcaaggtc gaggccgccg cggcgacgct gaacctgaca ccctatctcg accgaaagcc
     6241 gcgcgaactt tccggcggcc agcgccagcg cgtcgcgatc ggtcgtgcca tcgtgcgcga
     6301 gcccaaagct tttctgtt
//
LOCUS       DATNOI010000171         3354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_51695,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000171 DATNOI010000000
VERSION     DATNOI010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51695"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VN968_06610"
     CDS             <1..719
                     /locus_tag="VN968_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL28943.1"
                     /translation="AVLAASGRVMIRPRGFRLTSTVHHLEQVCWRAVPIVVLITFLIG
                     CIISQQGIFHFRKFGADIFVVDMLGVLVLREIGVLLVAIMVAGRSGSAYTAELGSMKM
                     REEIDALRTMGFDPIEVLILPRMLALIIALPILAFLGAMAALYGGGLVAWLYGGVDPE
                     AFLLRLRDAISIDHFTVGIIKAPVMAAVIGIVACVEGLAVQGSAESLGQHTTSSVVKG
                     IFFVIVMDGVFAIFFASIGM"
     gene            722..1492
                     /locus_tag="VN968_06615"
     CDS             722..1492
                     /locus_tag="VN968_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28944.1"
                     /translation="MAPETQNAIIRVRDISVQFGSTKVLDGLNLDVQRGEILGFVGPS
                     GAGKSVLTRTIIGLVPKVSGSIEVFGVDLDAADAAARRGVERRWGILFQQGALFSSLT
                     VRQNIQFPVREYLNVSQRLLDEITVAKLGMVGLRPEVADRFPSELSGGMIKRVALARA
                     LALDPELVFLDEPTSGLDPIGAGDFDELVRTLQRTLGLTVFMVTHDLDSLYTACDRIA
                     VLGNGKIIAAGSMADMQASQHPWLRAYFHGKRARAVMG"
     gene            1629..2498
                     /locus_tag="VN968_06620"
     CDS             1629..2498
                     /locus_tag="VN968_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXL28945.1"
                     /translation="METRANFVLIGSFTLAVIAAAFGFVLWFQSLHTTKARSPLRIVF
                     EGPAAGLRNGGSVNFNGIRVGEVISVKLDNPRRVVALAMVENNAPLRKDTLVGLEFQG
                     LTGVAAISLKGGEEAAPPVPLDEDGVPTLTADPNALQDVTEAIRGTLQNVNRLVADNQ
                     EAVKNSLHNLETFTTSLARNSEKIDSVMLKVDGVMGKADNLMLGLNTLAGGKEGGELF
                     QAVKSIRELAEDFDKRSGLLMADGRRTLADIGRAVNNLDRNPTRLLFGPANASTPEPP
                     KPAPAAAAERKRQ"
     gene            complement(2586..>3354)
                     /locus_tag="VN968_06625"
     CDS             complement(2586..>3354)
                     /locus_tag="VN968_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="negative regulator of septation ring formation"
                     /protein_id="HXL28946.1"
                     /translation="ATRNELRRGVLEMPQEAAESTAQMRKVIVDQIEALAELNRIVAH
                     HGRGLDVVSEGRSSSHREEESMLVAAGGRGAPRMREGGSASNLPPPDLGGPPSRRTEA
                     PPVSPASADQGRDGWLSDLLSRTDAGAGNRDVPRGRPQQGDGNPLESLSLDIGRLMDR
                     NLAAEMWDRYQRGESKAFSKRLYTPAGQKAFDEVARKYRSDRNFKQTVDRYIVEFERL
                     LDEVARDEGGAAALRGHLISETGLVYTLLAHAAGRLG"
BASE COUNT          559 a         1016 c         1118 g          661 t
ORIGIN      
        1 gcgctgtgct cgccgcgagc ggcagggtga tgatccgccc ccggggcttc cgcctgacgt
       61 cgaccgtgca tcatctcgag caggtatgct ggcgcgcggt gccgatcgtc gtgctgatca
      121 cgtttctgat cggctgcatc atctcgcagc aaggcatctt tcatttccgc aaattcggcg
      181 ccgatatctt cgtcgtcgat atgctgggcg tgctggtgct gcgcgagatc ggcgtgcttt
      241 tggtggcgat catggtggcg gggcgttccg gcagcgccta cacggcggaa ctcggctcca
      301 tgaaaatgcg cgaggagatc gatgcgctgc gcaccatggg gttcgatccg atcgaggttc
      361 tgattctgcc gcggatgctg gcgctgatca tcgcgctgcc gatcctggcc tttctcggcg
      421 ccatggcggc gctctatggc ggtgggctgg tggcgtggct gtacggaggc gtcgatcccg
      481 aggcgttcct gctccggctg cgcgatgcga tctcgatcga tcatttcacc gtcggcatca
      541 tcaaggcgcc ggtgatggca gcggtgatcg ggatcgtcgc ctgcgtcgag gggcttgcgg
      601 tgcagggcag cgccgaatcg ctcggccagc acaccacgtc ctcggtggtg aaggggatct
      661 ttttcgtcat cgtcatggat ggcgtgtttg cgatcttctt cgcctcgatc ggaatgtgac
      721 gatggcgcct gaaacccaaa acgccatcat ccgggttcgc gacatctcgg tgcagttcgg
      781 cagcaccaag gttctggacg ggctcaatct cgacgtgcag cgcggcgaga tccttggctt
      841 cgtcggtcct tccggcgcgg gcaagtcggt gttgacacgc accatcattg gattggtgcc
      901 caaggtcagc gggagcatcg aggttttcgg ggttgacctt gacgcggcgg atgccgcggc
      961 gcggcgcggc gtcgaacgtc gatggggaat cctgttccag caaggcgcgc tgttttcctc
     1021 gctcacggtg cggcagaaca tccagtttcc ggtgcgcgaa tatctgaacg tgtcgcagcg
     1081 attgctcgac gagatcacgg tggccaaact cggtatggtg ggcttgcggc ccgaggtcgc
     1141 cgaccgtttt ccctcggaac tctccggtgg catgatcaag cgggtggcgc tggcacgcgc
     1201 gctcgcgctc gatccggaac tggtgttcct cgatgagccg acgtctggcc tcgatcctat
     1261 cggggccggg gactttgacg agctggtcag gaccctgcag cgcactttgg ggctgaccgt
     1321 tttcatggta acccatgacc tggacagcct ttataccgct tgtgaccgca tcgccgtttt
     1381 ggggaacggc aagatcattg cagcaggatc gatggccgac atgcaggcct cgcagcatcc
     1441 ctggttgagg gcttatttcc acggcaagcg cgcccgcgct gtcatgggct agagcagata
     1501 cgacccggag gccgcgttcg cgcaaagtgg atgccggttt tgggaaaaga tcgtgcttga
     1561 gggattgggt taaacgacgg ccttgattca gcgtgctgaa ttcagtggct tggtatggga
     1621 gttggttgat ggaaacgcgg gcgaacttcg tcctgatcgg atcgttcacg ctggcggtga
     1681 ttgccgccgc gttcggcttc gttttgtggt ttcagtctct tcacaccacc aaggcgcgca
     1741 gccccttgcg catcgtcttc gaaggcccgg cggctggcct gcgcaatggc ggcagcgtca
     1801 atttcaacgg tatccgggtg ggagaggtga tctcggtaaa actcgataac ccgcgtcgag
     1861 tcgtcgcact cgccatggtt gagaataatg ccccgctccg caaggacacg ctggttgggc
     1921 tggaatttca gggcctgacc ggcgttgcgg cgatatcgct caagggcggc gaggaagccg
     1981 cgccgcctgt tcccctggat gaggatggag tccccacgct gacggctgat cccaacgcgc
     2041 tgcaggatgt caccgaggcg atccgcggca cgctgcagaa cgtcaacagg ttggtcgccg
     2101 acaatcagga ggcggtgaaa aattcgcttc ataacctaga gaccttcacc acgtcgctgg
     2161 cgcgcaattc cgagaagatc gatagcgtca tgctgaaagt cgacggcgtg atgggcaagg
     2221 ccgacaacct catgctgggc ctgaacacgc tggcaggcgg caaggagggc ggcgagctgt
     2281 tccaggccgt gaagtcgatc cgcgaactgg cggaggattt cgacaagcga tcgggtcttt
     2341 tgatggccga cggccgtcgc acgctcgccg atatcggccg cgcggtgaat aatctcgacc
     2401 gcaacccgac acgccttttg ttcggcccgg ccaacgccag cacgccggag cctccgaagc
     2461 cggcgcccgc ggcggcggcc gagaggaagc ggcagtagga cggtttcgat tacaacgccg
     2521 gttgcgaaac ccaaacaaaa cggaggcatc gctgcctccg ttttgttatg acatcagagt
     2581 gaaccctacc cgagccgccc cgccgcgtgc gcgagcagag tgtagacgag gccggtctcc
     2641 gaaatcagat gaccgcgaag cgcagcagcc ccgccttcgt cgcgcgcaac ctcgtcgagc
     2701 aggcgctcga actcgacgat gtagcggtcg accgtctgct tgaaattgcg gtcagagcga
     2761 tatttgcggg cgacttcatc aaaggccttc tggccggccg gcgtgtagag ccgcttggag
     2821 aaagccttgc tctcgccgcg ctgataacga tcccacatct cggcggcgag attgcgatcc
     2881 atcaggcggc cgatgtcgag cgacagcgac tccagcgggt tgccgtcccc ctgctgcggg
     2941 cggccacgcg ggacgtcgcg gttgccagcg ccggcatcgg tgcggctcaa caggtccgag
     3001 agccatccat cgcgtccttg atcggcgctt gcaggggaaa ccggcggcgc ttcggtgcgg
     3061 cgagacggag gcccgcccag gtctggcggc ggcaagttcg aggcgctgcc gccctcgcgc
     3121 atgcgcggtg caccgcgccc gccggcagcg accagcatcg actcttcttc gcgatgcgag
     3181 ctcgatcggc cctcgctcac gacgtccagc ccacggccgt ggtgggcgac gatgcggttc
     3241 agttccgcca aggcctcgat ctggtcgacg atcactttcc gcatctgcgc ggtgctctcg
     3301 gcggcctcct gcggcatttc cagcacgccg cggcgaagct cgttgcgggt ggcc
//
LOCUS       DATNOI010000172         3588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2374335,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000172 DATNOI010000000
VERSION     DATNOI010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2374335"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VN968_06630"
     CDS             complement(<1..426)
                     /locus_tag="VN968_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL28947.1"
                     /translation="MNLQFLIQLLVNGLVVGTLYGVVAMSFVLIYKATQVVNFAQGEL
                     LLVGAWVCWALLTKYQVPFWVGMPLTLVFMFAFGIAIQIVILRPMIGEPIISVIMVTI
                     GLSTVLQASLKWIFGVNPQPFPRVFTSQSVNFLGLQIQTV"
     gene            complement(494..2425)
                     /locus_tag="VN968_06635"
     CDS             complement(494..2425)
                     /locus_tag="VN968_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXL28948.1"
                     /translation="MMDYAGRLAHADTFAKLLRLNAREHGGEIALREKDFGLWRLFTW
                     NDYQTRVHDFALGLIELGLGRGDVIGIIGDNRPDWVSAEIATHAIGTMSLGLYRDVLD
                     EEAAYLLSYGEAKLVFAEDEEQVDKLLTLADRVPNLKHIIYSDPRGMRKYDDRCLMEA
                     DALAKLGRDRAAREPDLYDRLVDATKGEDVAILCTTSGTTANPKLAMLAAGRVLRHCA
                     TYLSFDPKGPDDEYVSVLPLPWIMEQVYVLGKGLLCRMKINFVEQPDTMMNDFREIAP
                     TFVLFAPRVWEQIAADVRAQVMDSSPLKQSLYELGMKTGLSALAQGKHSVVADILLFR
                     ALRDRLGFTRLRSAATGGAALGPDTFKFFQAMGVPLRTLYGQTELLGAYTLHPFGQVD
                     PDTTGVAMADNIEIRVENPDIHGVGEIVVRHPNMFLGYYKSPEAAAADMKDGWMHSGD
                     AGYFNGNKQLVVIDRIKDLAETARGERFSPQYLENKLKFSPYIAETVVLGAGRDALAA
                     MICIRFSIISKWAEKKRISFTTYTDLASRPEVYGLLRKEVETVNATLPPAQRISRFLL
                     LYKELDADDGELTRTRKVRRSVINEKYAGIIDAIYGGKRDIPIDTVIRFQDGTTQRIR
                     TTLKVVDLAQDVAAAEAAE"
     gene            complement(2422..3243)
                     /locus_tag="VN968_06640"
     CDS             complement(2422..3243)
                     /locus_tag="VN968_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL28949.1"
                     /translation="MEHALEVRGVSLRFGGVRALTEVSFGVSDGELFSIIGPNGAGKT
                     SIVNCISGRYRPTEGQLFYRGQDITALNPNARPSLGIGRTFQNLALFHHMSVLDNIMV
                     GRHHLLKNNFITGSPYWLTGARREELEHRRKVEEIIDFLDLQSVRKATAGTLPYGLRK
                     RVELARAMALEPQLILLDEPMAGMNFEEKEDMARYIVDLNEEFGMTVMMIEHDMGVVM
                     DISHRVMVLDFGRKIAEGDPAAVLADPHVKRAYLGEEDEVLVDPDDKPAAPECAA"
BASE COUNT          678 a         1091 c         1123 g          696 t
ORIGIN      
        1 gacggtctgg atttgcaggc ccaggaaatt caccgactgg ctggtgaaca cgcgcgggaa
       61 cggctgcgga ttgacgccga aaatccattt cagcgaagcc tgaagcaccg tcgatagccc
      121 aatggtgacc atgatcaccg agatgatcgg ctcgccgatc atcggccgca ggatcacgat
      181 ctggatggcg atgccgaacg cgaacatgaa caccagcgtc aagggcatgc cgacccagaa
      241 cggcacctga tatttggtca gcaacgccca gcacacccag gcaccgacca gcagcaattc
      301 gccttgcgcg aagttcacca cttgcgtggc cttgtagatc agcacgaacg acatcgcgac
      361 cacgccatag agggtgccga ccacgagacc gttgaccaga agctggatca ggaactggag
      421 gttcatgttg cttgacggat gtgagttgcg ggtttcttcc cctctccccc tgtgggagcg
      481 aattctctaa tcctcattcc gccgcctccg ccgcggccac atcctgcgcc agatcgacga
      541 ccttaagcgt ggtgcggatg cgctgggtgg tgccgtcctg gaagcggatc acggtatcga
      601 tcggaatatc gcgcttgccg ccatagatcg cgtcgatgat tcccgcgtat ttttcgttga
      661 tgacgctgcg gcggactttt cgggtgcggg tgagttcgcc gtcgtcggcg tcgagttcct
      721 tgtagagcag caggaaccgg gaaatgcgct gcgccggcgg cagcgtggcg ttgacggttt
      781 caacctcctt gcgcagcagc ccgtacactt cgggccgcga ggcgaggtcg gtgtaggtgg
      841 tgaacgagat gcgttttttc tccgcccatt tcgagatgat cgagaagcgg atgcagatca
      901 tcgccgccag ggcgtcgcgg ccggcgccga gcaccaccgt ctcggcgatg tagggcgaga
      961 atttcagctt gttctcgaga tattgcggcg agaagcgttc gccgcgcgcg gtctccgcga
     1021 gatccttgat gcggtcgatc accacgagct gcttgttgcc gttgaaatag ccggcgtcgc
     1081 cggagtgcat ccagccatct ttcatatcgg cggcggcggc ctccgggctc ttgtagtagc
     1141 caaggaacat gttgggatgg cgtaccacga tttcgccgac gccatggatg tccgggttct
     1201 cgacccggat ttcgatgttg tcggccatcg ccacgcctgt ggtgtcgggg tcgacctggc
     1261 caaacggatg caaagtatag gcgccgagca attcggtctg gccatacagc gtgcgcagcg
     1321 gcacgcccat ggcctggaag aatttgaagg tgtcgggacc aagcgcggca ccgccggtcg
     1381 ccgccgagcg cagccgggta aagccgagcc ggtcgcgcaa ggcgcggaac agcaggatat
     1441 cggcgaccac ggagtgcttg ccctgcgcca gcgccgacag tccggtcttc atgccaagct
     1501 cataaagact ttgcttgagc ggggacgaat ccatcacctg cgcgcgcacg tcggcggcga
     1561 tctgctccca taccctcggt gcgaacagca cgaaggtcgg cgcgatttcg cgaaagtcgt
     1621 tcatcatggt gtcgggctgc tcgacgaagt tgatcttcat ccggcacagc aggcctttgc
     1681 cgagcacata gacctgttcc atgatccagg gcagcggcag caccgagacg tattcgtcat
     1741 ccggcccctt cgggtcgaac gaaagatacg tcgcgcaatg ccgcaacacc cgccccgccg
     1801 cgagcatcgc cagcttcggg ttggcggtcg tcccggaggt ggtgcagagg atcgcaacgt
     1861 cctcgccctt tgtcgcgtcg accagccggt cgtagagatc gggctcgcgg gcggcacggt
     1921 cgcgcccgag cttcgcgagc gcgtcggctt ccatcaggca gcggtcgtca tatttccgca
     1981 tgccgcgcgg atcggaatag atgatgtgct tgagattggg tacgcgatcg gcgagcgtca
     2041 gcagcttgtc gacctgctct tcgtcctcgg cgaataccag tttggcttcg ccatagctca
     2101 acagatacgc cgcttcctca tccagcacgt cgcgatagag gcccagactc atggtgccga
     2161 tggcgtgggt ggcgatttcc gccgaaaccc agtccggccg gttgtcgccg atgatgccga
     2221 tgacgtcgcc gcgaccgagc ccgagttcga tcaggccgag tgcaaagtcg tgcacccgcg
     2281 tctgatagtc gttccaggtg aacaggcgcc agagcccgaa atccttttcg cgcagcgcga
     2341 tttcgccgcc gtgttcgcgg gcgttgaggc gcaacagctt cgcaaaagta tcggcgtggg
     2401 cgagacgtcc ggcgtaatcc atcatgccgc gcactccggg gcggcgggct tgtcgtcggg
     2461 atcgaccaga acctcgtcct cttcgccgag ataggcgcgc ttcacatggg gatcggccag
     2521 caccgcggcc ggatcgcctt ccgcgatctt gcggccgaaa tccagcacca tcacccggtg
     2581 ggaaatatcc atcaccacgc ccatgtcgtg ctcgatcatc atcacggtca tgccgaactc
     2641 ttcgttgaga tcgacgatgt agcgggccat gtcctccttt tcctcgaagt tcatgccggc
     2701 catcggttca tcgagcagga tcagctgcgg ctccagcgcc atggcgcggg cgagttcgac
     2761 acgcttgcgc aggccatagg ggagggtgcc ggcggttgcc ttgcgcaccg actgaagatc
     2821 gaggaaatcg atgatctcct ccaccttgcg gcgatgctcg agttcttcgc gtcgtgcgcc
     2881 ggtcagccag tacggcgatc ccgtgatgaa gttgttcttc agcaaatgat gccggccgac
     2941 catgatgtta tcgagcacgc tcatatgatg aaacagcgcg aggttctgga aggtacggcc
     3001 gatgccgagc gaagggcggg cgttggggtt gagcgcggtg atatcctgcc cgcgatagaa
     3061 caactggcct tcggtcggcc ggtagcggcc cgagatgcag ttgacgatcg aggtcttgcc
     3121 ggcgccgttg gggccgatga tcgaaaaaag ttcgccgtcg ctcacgccaa aactgacctc
     3181 ggtgagcgca cgtacgccac caaagcgcag tgagaccccg cgcacttcca aagcgtgttc
     3241 cacgcattcc ctccctggag cggtctcggg caaacgggtt tcgatcgccg tgagaaatcg
     3301 ctcgcgatca aaagctttcg gcgcttgaaa cgctctatcc ttggcgggtt cagctgcgcg
     3361 ccaagccgag cctacaatgg tcggactgct caacatattg gactattggg cgcagtttgc
     3421 ccagcgcgct ttgtcgcgca actggcacag ataccgtggc gaatgaccgt attgtgcccg
     3481 accggcactg ggactgattc gaggcggctg ttcgagacag cgattgaaca cgttccttcc
     3541 ggttaccccg ctccaaaagg aggagccgga atagaacaga ccgcccgt
//
LOCUS       DATNOI010000173        15368 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_827683,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000173 DATNOI010000000
VERSION     DATNOI010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15368
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827683"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..190)
                     /locus_tag="VN968_06645"
     CDS             complement(8..190)
                     /locus_tag="VN968_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28950.1"
                     /translation="MFNIVKRRDFMLADDLPTREETQRLCDEKYGLDHFVIETAQSIQ
                     RLRPSADGGSRMVKPA"
     gene            complement(427..594)
                     /locus_tag="VN968_06650"
     CDS             complement(427..594)
                     /locus_tag="VN968_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28951.1"
                     /translation="MFNIVKRRDFMLGQDVATAQEAEQRLRDQYDCDYIVVNRADVDP
                     VKEPQSPTLPR"
     gene            complement(873..1019)
                     /locus_tag="VN968_06655"
     CDS             complement(873..1019)
                     /locus_tag="VN968_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28952.1"
                     /translation="MMKPPGEQTLVAVTVKPLAFHVVLHQKVQNAELNLTAVTATPKR
                     RSNA"
     gene            1199..1864
                     /locus_tag="VN968_06660"
     CDS             1199..1864
                     /locus_tag="VN968_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28953.1"
                     /translation="MESHQTSLAPRDRAGIACDASWDFGAPARQRLRSCLVRLRKMPS
                     TTLLRIFRRFQSPGGDEGYRVAFEKLEEEASHLRRGYDGYRTAFQTLQQEATQLRQGF
                     DGYRAAFEALSAEVSQIRDERDRVLKTLHKVSRLRRSLPSGLDGRQLCFLHIGKTAGT
                     SIQHALFEALWDAAIFHDSLPGFDRVTPAELALNDDLLARSHRSLDLELPFFENEIAA
                     FKI"
     gene            1782..2327
                     /locus_tag="VN968_06665"
     CDS             1782..2327
                     /locus_tag="VN968_06665"
                     /inference="COORDINATES: protein motif:HMM:NF018669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HXL28954.1"
                     /translation="MTFLRDPIDRLISNYHFLRTRSPLSRYSEAALQAARSLSFKDFL
                     LCDDRNVRMITANFQSKALAHDIRPDFLDETMTGALLENAERNLGTFDFIGICEYFDD
                     SIHVLSEMLGVELSAKKLNVNPDRRFTEPTAEELDIARSLNGLDIQLYGKARSRFERI
                     YLSRSAPGSFHSPAGALGGSV"
     gene            complement(2324..2641)
                     /locus_tag="VN968_06670"
     CDS             complement(2324..2641)
                     /locus_tag="VN968_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28955.1"
                     /translation="MSYDSRDRHKHNGDRLTTFPSKKTPASGRMLFCSPSGGVGTDTV
                     DYSAVEYSASSAGATVNLTHTGAQSDGDAAGDILSNFENVVRNGATINFTSRSPGAAG
                     GKD"
     gene            complement(2770..4647)
                     /gene="recQ"
                     /locus_tag="VN968_06675"
     CDS             complement(2770..4647)
                     /gene="recQ"
                     /locus_tag="VN968_06675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HXL28956.1"
                     /translation="MPVPAIGSARNARSDTAPDALSVLNSVFGLPAFRGAQEAIIRHV
                     TDGGNCLVLMPTGGGKSLCYQLPSLLRQGCGIVVSPLIALMRDQVAGLLEAGVRAAVL
                     NSTLSFSEAAAVEARLLAGDLDLLYVAPERLVTPRCLAMLDRAPIALFAIDEAHCVSQ
                     WGHDFRPEYISLSVIAERFPDVPRVALTATADGLTRREIVDRLGLADAPHFVASFDRP
                     NIRYEIVDKQNAPAQLKTFITERHAGDAGIVYCLSRAKVEDTAAALAKAGVQALPYHA
                     GLDANLRARNQDRFINEDGVVIVATVAFGMGIDKPDVRFVAHLDLPKSIEAYYQETGR
                     AGRDGKPSSAWMAYGLSDIVQQRRMIDESTGAEAFRRVSIRKLDALVGLAETASCRRT
                     RLLGYFGETPVTAKCGNCDNCLSPPRVRDGKVIAQKLLSCAYRTGQRFGAMHLIDVLV
                     GRLTDRVKQFGHDKISVFGIGAELNEKQWRAALRQLVAMGHLHPDSEAFGALKLTETA
                     RGVLKGETEVMLREETAGARIRTSRAKSRRGDLAPQPAGQAGTGNPALLGALRAWRSE
                     VARTRGVPAYVVLHDSTIDGIAASRPTTLEQLRGIPGIGDKKLEHYGNELLALVKAAE
                     N"
     gene            complement(4860..5024)
                     /locus_tag="VN968_06680"
     CDS             complement(4860..5024)
                     /locus_tag="VN968_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28957.1"
                     /translation="MVDKPSDAAWAALEAANDLGDDATVAACRRVIDANLSGALASPS
                     DLHIVLDYFR"
     gene            complement(5192..6316)
                     /locus_tag="VN968_06685"
     CDS             complement(5192..6316)
                     /locus_tag="VN968_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HXL28958.1"
                     /translation="MNDAKTFLQDLDDAVSRGTAESCLHAVRHATNLLIAGRYSDDET
                     CTFGEVIGRLADEIEVAARAELATRLAQINHAPINIIHKLAFDDSIDVAGPVLRNSER
                     LDANTLVANAQTKSQPHLLAISKRKSLEHSVTDVLVTRGNREVVNSVANNAGARFSGS
                     GFLHLIKRAEGDSILAEQLGLRKDIPRHLFQQLIAKASDDVKKRLEQERPGMVDQIQA
                     SVTDVTGALQSKFGPASRSYYVAKRLVATQHQHGNLNENSIYDYARSHKLDEVTIGLS
                     LLCALPSDVIERALLDKNREMLLVLTKALDFSWATTMALLFLGAKDHRITALDLTDLE
                     NQFARLNVETSRSVLELYQTRKSAAVTETSTNRLAPHHMH"
     gene            complement(6649..8319)
                     /locus_tag="VN968_06690"
     CDS             complement(6649..8319)
                     /locus_tag="VN968_06690"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXL28959.1"
                     /translation="MRHRHFFAAASSAFAMLLSGATLAQTQGPALTGLVSSAEENAME
                     GVLVSATKEGSTVTFTVVTGKDGRFSFPASKLDPGQYVLRVRAVGYELDHHDAVEIPE
                     QKAATLDLKLRKTADLAAQLSNGEWLASIPGTDQQKGQLLNCVGCHTLERIVRSKYDS
                     DAFLTQILPRMQGYVNQSIPAHPQLRRAERLMEERGDQRVQVYRALADYLGTINLSAG
                     SPWSYPLKTFARPTGRATRVIYTEYRLPRETISPHDVIADADGIVWYSSFGEQNLGRL
                     DPKTGKVTEYPVPEHKPDFPTGFLGLRADKAGNLWLGNMYQATIVKFDRKAGSFQFWP
                     LPSEQNIDAAQVNMASAQSSHVDGKVWAQNNGFAGVHRLDVATGKIETWEPFKNAPKG
                     EPHNIYDVIPDSKNNAYFTDFRQQHIGRVDAVTGEVRLFEIPTARSAPRRGSMDAQDR
                     LWFGEYRGDRIGMFDTRTEQFKEWKLGTRWASPYDVTLDRNEEAWTGSMVTDQVTRLN
                     TRTGEMVDYLLPRSTNIRRVFVDNTTTPVTFWTGSNHGASIVKVEPLD"
     gene            complement(8430..8807)
                     /locus_tag="VN968_06695"
     CDS             complement(8430..8807)
                     /locus_tag="VN968_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXL28960.1"
                     /translation="MIDAMRATAALVMTLLLAGAARADGDAARGEARFQDCAACHRLE
                     AGANNVGPSLHGIFSRKAGELADFRYSPAIKRSGITWTPEALDKFITDPQAAVPANRM
                     PYAGMSNASDRADLIAYLSKASR"
     gene            complement(8804..10579)
                     /locus_tag="VN968_06700"
     CDS             complement(8804..10579)
                     /locus_tag="VN968_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXL28961.1"
                     /translation="MTTKLKEVDVVIVGLGWTGGILSKELTEAGLKVVALERGAPRSS
                     ENDYSLPNIRDELRYVVRHDLMQDTARDTITVRNNPQQEALPMRRLGSFLPGEVVGGS
                     GVHWSGHTWRWTDMEFKIRSNYEERYGKKFIPDDMTIQDWGITYAELEPYYDKFEYTA
                     AVSGKAGNIGGQILSGGNPFEASRARDYPLPPLTPILASEMFSDAARNAGYHPFPRPS
                     ANASQPYTNPDGSKFGGCQYCGYCQRFGCEANAKGSPHLTVIPIAMRNPNFELRTHSW
                     VTKVLKDSDGRRVTGVSYTNVLNGEEFEQPATIVLLCAYAINNVHLMLLSGIGTPYDP
                     VAQKGVIGKNYCYQTGAAATLFFDGKYFNPFMNAGGSNATIDDFNTNWAFDRAPHDFV
                     GGYNVSGGFNTPLPIGYRPVPSGTPLWGSEWKRATAKWYQTAMVISASGSVMANRYNC
                     YDLDSTYRNAFGQPLMRMTFDYQSNEHKMGRHAAGLINDIAKSMNPTRLNPASARTDP
                     WTVVPYQSTHNTGGTIMGTNPRDSALNKYLQSWDCHNLFVVGANVFPHNSSYNPTGPV
                     GALAYWTADAIKNRYVKNPGPLVQA"
     gene            complement(10590..11318)
                     /locus_tag="VN968_06705"
     CDS             complement(10590..11318)
                     /locus_tag="VN968_06705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HXL28962.1"
                     /translation="MTEQKLDRRDFLKTAVVGGAAAAGGSAAIVTPETAQAEPAAPAA
                     TAVAPQAAGYAFLNLDEAAFVESLVDHMVPADEISPKGTDLGVNVYIDRALAGAWGKG
                     ERLYMQGPWRQGTSSQGYQLPLTPAQLYRAGIEATNAHCRKAYGKSFDRIEDAQRQEV
                     LVGLSTGKIVFDNGLPVRVFWATLYQTVIEGMYSDPIYGGNRNKAGWAIIGFPGIIAV
                     HRDHVEKYRGKPFPTTPLGISDMS"
     gene            11415..11885
                     /locus_tag="VN968_06710"
     CDS             11415..11885
                     /locus_tag="VN968_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28963.1"
                     /translation="MATMSPADNARRFVHRKSARRSSAAQVSDGVGGKVLGDAAGLVF
                     AILRAPTVLRTQQGFRMACRDEPAVIVTDYFMPNGNAQYLLTRLRTTAATANIPVIVL
                     SGQQLNDMTVQSLGREICGHPGATQILRKSQDTYALFETLKKFCGFEREPVNLV"
     gene            complement(11903..12535)
                     /locus_tag="VN968_06715"
     CDS             complement(11903..12535)
                     /locus_tag="VN968_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXL28964.1"
                     /translation="MLEAVIWDFGGVLTTSPFEAFARFETERGLPADIIRRTNANNHW
                     ENAWAKFERAEVDIETFDQLFAAESLALGAEVRGKDVLPLLSGDLRPEMVEALRRIGT
                     EFKTGCITNNLPANAIGSLGGRSLYVAEVMALFDHVIESAKIGLRKPDPRIYRMMAEK
                     LGVDPKACVYLDDLGVNLKPAREMGMTTIKVLNASQAIAELETATGVSLR"
     gene            12649..13410
                     /locus_tag="VN968_06720"
     CDS             12649..13410
                     /locus_tag="VN968_06720"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HXL28965.1"
                     /translation="MKILLLTVAGLALILCAPAYAIVGGGVPSADGIGRSVVTIVGSR
                     GNFCSGALIAPKLVLTVAHCVQPGATYKIVEYGADKQPQLHDVKGVAIHPSFNLQAMM
                     AHRATADVALLQLEASPKGKNASLLGVPQAPMVAGSRFTIAGIGVTARGEGKSGGTIR
                     VAGLVATGRPGSLQIRLVDPVGQGARDGLGACTGDSGGPVFEDRQIGPAIVGVISWST
                     GPNGAAGCGGLTGVTPLTLYRDWIVQTARQWGEAL"
     gene            complement(13421..14263)
                     /locus_tag="VN968_06725"
     CDS             complement(13421..14263)
                     /locus_tag="VN968_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXL28966.1"
                     /translation="MKASASNLMIGSTTLAAIAAAFVGFLGFQKIHGIAQRGPLRIVF
                     EGSASGLRQGGNVNFDGVQVGEIRSLKLDNPRKVVALAMVDNSAPIRKDTVVGLEFQG
                     LTGVAAISLTGGAAAAPPVPLDKDGIPTLTADLSEVQSIRDTLHSVDRILVGNQAMLK
                     DTLSNFETYTATLASKGDAIDSIIRKADGAFESFDGAMAKIDNLVPGLGSGSADELYD
                     KVRTIRELAESFNKRSGAFMEEGRRSLLDISQAAIKVTRKFDPQAVSGENPPPPWKPN
                     QKRH"
     gene            14451..14606
                     /locus_tag="VN968_06730"
     CDS             14451..14606
                     /locus_tag="VN968_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28967.1"
                     /translation="MPKLLSTDEVADYRDRGYHFPIDALSAGEVTKFRRRRLPSMSTK
                     RRPSSSN"
     gene            14633..14905
                     /locus_tag="VN968_06735"
     CDS             14633..14905
                     /locus_tag="VN968_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28968.1"
                     /translation="MLLFHGSEPNRSDDRRIGLAIRYIPTHLKQAVGQKDWATLVRGK
                     DSYGHFQPEYVPKRDLEPEALAFHKAVSEEQVRVLYRGTGKTAYRA"
     gene            complement(14973..>15368)
                     /locus_tag="VN968_06740"
     CDS             complement(14973..>15368)
                     /locus_tag="VN968_06740"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXL28969.1"
                     /translation="VQLQPATAQTLALALHELVTNSAKYGALSTLSGRLSIGWEMQGD
                     FLMLAWEETGGPMVRKPTSRGFGTRSVIASIESQLGGKALFDWRSEGLICRLLVPLVR
                     QAATPEQDDPPDAMAFDPVLRVVSGSAGS"
BASE COUNT         2823 a         4619 c         4789 g         3137 t
ORIGIN      
        1 tgccacgcta ggccggtttc accattcgtg atccgccgtc agccgatggg cggaggcgct
       61 gaattgattg cgccgtctcg atgacgaagt gatccagacc gtatttctcg tcgcacaatc
      121 gctgtgtttc ttcccgggtc ggcaaatcat ccgccagcat gaaatcccgg cgcttgacga
      181 tattgaacat tcgttctttc caaccccctg cagtgaggcc gggattattc gccaaaaaaa
      241 ttacgttgtg aatccgtcaa ccgggggaga aaacgaacat cgggaacgag cgccgcgatc
      301 acggtcgccg agagcaatca ctgcacgatc atcgggaatg tctgcaccag gtgtttcagg
      361 cgacggagtg tcgccgcagt ccacgcgcct ggcgcagccg agccaaggac aattcgccga
      421 tgcggctcaa cgcgggagcg tcggcgactg gggttctttg acgggatcga cgtcagctcg
      481 gttcacgaca atgtagtcgc agtcgtattg atcccgtagc cgctgctcgg cttcctgcgc
      541 ggtcgccaca tcctggccaa gcatgaaatc tctacgctta acgatattga acatggcagc
      601 acccctaaat tgtacgcagc accaatcaaa attcagcagc gagtcgcctt aactaaactc
      661 caacatcgca ttacgaaatt acggcgattt ggtgattttg ttttgagcgc gagcgcagcg
      721 gtatctttgt cacatctccc tcttaagttt ccgttttccc ccatgcgcat ctttgcgcga
      781 tgtatttaac actcgctcga cgtgccgatt gagcgcccgc atcatgccga tgcgcgccat
      841 catggtgggt ccattttgct cggcgaccag gatcaggcat tcgatcgccg cttcggggtc
      901 gctgtcacgg cagtaaggtt aagttcggca ttttggactt tttggtgcag caccacatgg
      961 aatgccaacg gcttcacggt cacggccacc agcgtctgct cgcccggcgg cttcatcatc
     1021 accagtggca tcctgaacgt cgttcgcgca ccaacacgca acctgaacgg gcgatcagca
     1081 gcaataggca agcgagggga ttgctgcacc gcgtgcccag gccgtgcagt acagcagggc
     1141 ttgccgagat tcgaaatatt ttcgaaatca gattaacaac tcgtaatttg cacggccgat
     1201 ggaatcccat cagacttcgt tggcaccgcg cgatcgggct gggatcgcat gcgatgcgtc
     1261 atgggacttc ggcgcgccgg cacgccagcg cttgcgcagt tgcttagtgc ggttgcgtaa
     1321 aatgccatcg acgactctgc tgcggatttt ccgccgtttt caatcaccag gcggagatga
     1381 gggttatcgg gtagcgtttg aaaaattgga ggaagaggca tcccatctgc gccgcggata
     1441 tgacggctat cggaccgcct ttcagacgct ccagcaagag gcaacccaac tgcggcaagg
     1501 ttttgatggc tatcgtgcag cattcgaagc acttagtgca gaggtctctc aaatacggga
     1561 cgagcgagat cgcgtcctga agacccttca caaggtcagc cggctgcgga gatctctgcc
     1621 ttccggcctc gatggaagac aattgtgttt tctgcacata ggaaaaactg ccggcacttc
     1681 gattcagcac gctttgttcg aagcgttatg ggacgccgcg atcttccacg attcgctgcc
     1741 cgggtttgac agagtgacgc cagcagaatt ggcgctgaac gatgaccttc ttgcgcgatc
     1801 ccatcgatcg cttgatctcg aattaccatt ttttgagaac gagatcgccg ctttcaagat
     1861 atagcgaggc ggccttgcag gcggctcgat ccctgtcctt taaagacttc ctgctttgtg
     1921 acgatcgcaa cgtccgaatg atcaccgcga attttcagag caaggcgctt gcgcatgaca
     1981 tccggccgga ttttttggat gagacgatga ccggcgcttt gctcgaaaac gccgagcgaa
     2041 atttgggaac attcgatttc attgggatat gcgaatattt cgacgacagc atacacgtgc
     2101 tgtcagaaat gctcggtgtc gagttgtctg ccaaaaagct gaatgtcaat ccagatcggc
     2161 gcttcactga gccaactgcc gaagagctgg acatcgcgcg atcgctcaat gggttggaca
     2221 tccagttgta cggcaaggcc cggtcgcgct ttgagcgcat ttatctaagt cgcagcgctc
     2281 caggttcgtt tcattccccg gcaggcgcgc tcggcggcag cgtctaatcc ttgccgcccg
     2341 ccgcaccagg tgacctgctc gtaaaattga tcgtcgcccc gttgcgaacg acgttctcga
     2401 agttggaaag gatatcgccg gcggcgtcgc cgtcgctctg cgccccggta tgggtcaggt
     2461 tcacggtggc gccggccgaa gatgcggaat attcaactgc ggaataatca acggtgtcgg
     2521 tgccgacgcc gccggaaggc gagcaaaaca acatccgacc tgacgcgggc gttttcttgg
     2581 acggaaaggt cgttaaccga tcgccattat gcttgtggcg atcgcggcta tcgtagctca
     2641 taccgcgagg gaaacccccg acctaaagcc cgccgtctca actcggcggg ttttttgcgt
     2701 tgctccaata tcccttcagt cgagccgccg caccacccgc cctcgctcgc gcttcggccc
     2761 gcgagccgat cagttctccg ccgccttcac cagcgccagc agttcgttgc cgtaatgctc
     2821 gagcttcttg tcgccgatgc cggggatgcc gcgaagctgt tcaagcgtcg tcggtcgcga
     2881 cgccgcgatg ccgtcaatcg ttgaatcgtg cagcacgaca taggccggca cgccgcgcgt
     2941 gcgcgcgact tccgaacgcc acgctcggag agcaccgagc agggctggat tgccggttcc
     3001 cgcctggccg gcgggttggg gtgcaaggtc gccgcgcctt gatttggctc tactggtgcg
     3061 aatgcgggcg ccggcggttt cctcgcgcag catcacctcg gtctcgccct tcagcacgcc
     3121 acgcgcggtc tcggtcagct tcaacgcacc gaaggcctcg ctgtcgggat gcagatggcc
     3181 catcgccacc agctggcgca gggcggcgcg ccactgcttc tcattgagtt cggcgccgat
     3241 gccgaacacg gatatcttgt catgcccgaa ctgttttacg cggtccgtca aacgcccaac
     3301 cagaacatcg atcaggtgca tcgccccaaa gcgctgcccg gtgcgatagg cgcaggacag
     3361 cagtttttgc gcgatcacct tgccgtcgcg aacgcgcggt ggcgacaggc agttgtcgca
     3421 attgccgcat ttcgcagtca ccggcgtctc gccgaaatat ccgagcaggc gggtacggcg
     3481 gcagctcgcg gtttcggcga gaccaacgag cgcgtcgagc ttgcggatcg acacccgcct
     3541 gaacgcttcg gcgcccgtgg actcgtcgat catgcgccgc tgctgcacga tatcggacag
     3601 gccatacgcc atccaggcgc tggacggctt gccgtcacgc ccggcgcggc cggtctcctg
     3661 ataataggcc tcgatgcttt tcggcaagtc cagatgtgcg acgaagcgca cgtcaggctt
     3721 gtcgatgccc atgccgaacg cgacggtcgc aacgatcacg acaccgtcct cgttgatgaa
     3781 gcgatcctga ttgcgggcac gcaaatttgc gtcgaggccg gcgtgatagg gcagcgcctg
     3841 cacgccggct ttggccaggg ccgccgcggt gtcttccacc ttggcgcgcg acaggcagta
     3901 gacgatgccg gcgtcgccgg cgtggcgttc ggtaatgaaa gtcttcagct gcgctggggc
     3961 gttctgcttg tcgactattt catatctgat attgggacga tcaaagctcg cgacaaagtg
     4021 gggcgcgtcg gcaagtccta gtcgatcgac gatttcccgg cgggtgaggc catcggcggt
     4081 ggcggtaagg gcgacgcgcg gcacgtcggg aaaacgttcc gcgatgaccg agagactgat
     4141 atattccggg cggaagtcgt gcccccattg cgatacgcaa tgcgcctcgt cgatcgcaaa
     4201 caaggcgatc ggcgcgcggt ccagcatggc aaggcagcgc ggtgtcacca gtcgctcggg
     4261 cgcgacgtag agcagatcaa gatcgccggc caggagccgg gcctcgacgg cggcggcttc
     4321 gctaaaggac agcgtggaat tgagcaccgc cgccctgacg ccggcctcga gcaatccggc
     4381 gacctgatcg cgcatcagtg cgatcagcgg cgacacgacg atgccgcaac cctggcgcaa
     4441 cagcgacggc aattggtagc acagcgactt gccgccgccg gtcggcatca gcaccaggca
     4501 attgccgccg tccgtgacat gccggatgat cgcttcctgc gcgccccgaa aggcgggcag
     4561 gccgaacacg gaattcagga cggaaagtgc atcgggggcg gtgtcggacc tggcgttacg
     4621 ggcggagcca atggcgggaa cgggcatgcg gatcaggtcg tccgattaga agcgtgcgcg
     4681 ggtcggcctt ggggcgacgg caccggatcg atggatcccg gcggtagccg tgtcaatgat
     4741 tatgtcaggt ctgttgctga ttcggaccgt ctccatattc gggtccaaat tggttttgcg
     4801 caaatatgcg ccggaaaccg ccgaaaggcc ggctttccct gctcaagacc gccttgtcgt
     4861 cacctgaagt aatcgaggac gatgtgcaaa tcggatgggg aggcgagcgc gccgctcagg
     4921 ttcgcatcga tgacgcggcg gcacgctgcg acggtcgcat cgtccccgag atcgttcgcg
     4981 gcttccagag ccgcccaggc cgcgtccgag ggtttgtcaa ccatgagccc gtcagacgtg
     5041 agtctatcag acgtgagtct attagacttg gccctatcac acttgggcct atcagacttg
     5101 ggctcaccgg aaatcttgcc gaagcgtgcg ggtgcggctg cgccgaacag gattgcgttc
     5161 atggtcaatg ctccacagcc aatctcatat ctcaatgcat gtgatgcgga gcgaggcggt
     5221 tcgtactggt ttcagtaacc gcggcactct tgcgtgtctg atagagttcc agaacgcttc
     5281 gggatgtctc gacgttgagg cgggcaaact ggttttccag gtcagtcagg tccagcgcgg
     5341 tgatgcggtg atcttttgcg ccgagaaaga gcagtgccat tgtcgtcgcc cacgaaaaat
     5401 cgagggcttt ggtcaagacc agcagcatct ccctgttctt gtccagcaag gcgcgttcaa
     5461 tcacatcgct cggcaaggcg cacagcaagg atagtccgat cgtgacttca tcgagcttgt
     5521 gggacctggc atagtcataa atgctgttct cgttcagatt gccatgctgg tgctgcgtcg
     5581 ccaccagcct ttttgcgaca taataacttc ttgatgccgg accgaacttg gattgcagcg
     5641 cgccggtgac atcggtcacc gacgcctgaa tttggtcgac catcccgggg cgctcctgtt
     5701 cgagtcgctt cttgacgtcg tccgatgcct tcgcgatcag ttgctggaac agatggcgcg
     5761 ggatgtcctt gcggaggccg agttgctcgg caagaatgga atcgccctcg gcgcgcttga
     5821 tcaagtgcaa gaagccggaa cccgagaaac gcgcgccggc gttatttgca acggagttca
     5881 ccacctcccg attgccgcgg gtcacgagca cgtccgtgac cgagtgctcg agggattttc
     5941 gtttcgagat cgcgagcaga tgcggctgac tcttggtctg ggcgttggca accagggtgt
     6001 tggcatcgag acgttcggag ttccggagta cggggccggc gacatcgatc gaatcgtcga
     6061 aggcgagttt gtggatgatg ttgatcggtg cgtggttgat ctgcgccagc cgtgttgcaa
     6121 gttcggcgcg ggccgcaact tcaatctcat ccgcgagccg cccgatgacc tcgccaaagg
     6181 tgcatgtctc gtcgtcgctg taccggccgg caatcaatag attggtcgca tgccgcaccg
     6241 cgtgcagaca gctttcggcg gttcctcgtg atacggcatc gtcgagatct tgtagaaacg
     6301 tctttgcgtc attcatgttc ggcaggcctc aaatcgcagc gcatgcgcat aatactgtcg
     6361 gctaggttca atcgtacccc ggcaaacaca acttttcagt aaaggagcaa aaaagacgac
     6421 gtcgctaaat attcgctaaa atgcgcatca gtcggttggc ttgtcggtta ggcggatccc
     6481 cgcaaggttg cggttgttcg ggagctggac tgcggcggcg ggtgcgaagc cggcattccc
     6541 cgctcgaaac cgcattttct tcggcgacca tcgcagctgt gacctcatcg cggtctcaag
     6601 ccggtgatgg ctagcgacgc gtcgagcgct tcgatggtga gcaggcggtc aatccaatgg
     6661 ttcgactttg acgattgaag cgccatgatt gctgccggtc cagaaagtga ccggcgttgt
     6721 ggtgttatcc acgaaaactc gccggatgtt ggtcgagcgc ggcaacagat aatcaaccat
     6781 ctcgccggtt cgggtgttca gacgcgtcac ctggtccgta accatcgagc cggtccacgc
     6841 ctcctcgttc ctgtcgaggg tgacatcgta gggcgatgcc cagcgtgtac cgagcttcca
     6901 ctccttgaac tgttcggtcc gggtgtcgaa cattccgatg cgatcgcccc ggtattcgcc
     6961 gaaccacagc cggtcctgcg cgtccatgct gccgcgccgc ggcgcggagc gagccgtggg
     7021 aatttcgaac agtctgacct cgccggtcac ggcgtcgacg cgtccgatgt gctgctgacg
     7081 gaagtcggtg aaataggcat tgtttttcga gtccgggatg acgtcgtaga tattgtgcgg
     7141 ctcgcccttg ggggcgttct tgaacggctc ccacgtctcg atcttgccgg tcgcgacgtc
     7201 gaggcggtgc acgccggcaa agccgttgtt ctgcgcccag accttgccgt cgacatgcga
     7261 gctttgcgcg ctcgccatat tgacctgcgc ggcgtcgatg ttctgttctg acggcaacgg
     7321 ccaaaactgg aatgacccgg ccttgcggtc gaatttgacg atcgtcgcct gatacatgtt
     7381 gccaagccac aaattgccgg ccttgtcggc gcgcaggccg agaaagccgg tcgggaagtc
     7441 gggcttgtgc tcgggaaccg gatactccgt gacctttccg gtcttgggat cgagcctgcc
     7501 gagattctgt tcgccgaaac tcgaatacca gacgatgcca tccgcatccg cgatcacgtc
     7561 gtgcggcgag atcgtctcgc gcggcagacg gtattccgta tagatgaccc gcgtcgcccg
     7621 gccggtcggg cgcgcaaagg tcttgagtgg atagctccac ggcgaaccgg cgctgagatt
     7681 gatggtgccg agatagtccg cgagcgcgcg gtagacctgg acgcgctggt cgccgcgctc
     7741 ctccatcaaa cgctcggcgc ggcgcaattg cggatgcgcc ggtatgctct ggttcacgta
     7801 gccctgcatg cgcggcagga tctgcgtgag aaaggcatcg gagtcgtatt tcgagcgcac
     7861 gatgcgctcg agcgtgtggc agcccacgca gttgagcaac tggccctttt gctgatcggt
     7921 gccgggaatg ctcgccaacc attcgccatt ggacaattgc gcggcgaggt ctgcggtctt
     7981 gcgcagcttg aggtccagcg tcgcggcttt ctgctccggg atttcaacgg cgtcgtgatg
     8041 gtccagctcg tagccgaccg cgcgcacgcg gagcacatat tgacctggat ccaacttgct
     8101 cgccgggaag ctgaagcggc catccttgcc ggtgacgacc gtgaacgtga cggtcgagcc
     8161 ttccttggtg gcgctcacca acactccctc catggcgttt tcctcggcgg aactgacgag
     8221 gccggtcagt gcggggcctt gcgtctgcgc cagggtggcg cccgaaagca gcatggcgaa
     8281 cgccgaactg gcggcggcga aaaagtgacg atggcgcatg ggttcctccc ggaaggtgtt
     8341 gttcttcttg cttcttgccg gtacaggccc tgcggcctgc acgtcgtaac cagtcgtctg
     8401 cgcgccgggc ggtccgctgt tcgggccgac tacctcgatg ccttcgacag atatgcgatg
     8461 agatcggcgc ggtcgctcgc gttcgacatg ccggcatagg gcatgcggtt ggccggcacc
     8521 gcggcttgcg gatcagtgat gaacttgtcg agtgcctccg gcgtccaggt aatcccgctg
     8581 cgtttgatcg ccggcgagta gcggaaatcg gcgagctcgc cggccttgcg cgaaaaaatg
     8641 ccgtgcaagc tcggcccgac attattggcg ccggcctcca atctgtggca ggcggcgcaa
     8701 tcctggaagc gcgcttcgcc gcgcgcggcg tcgccgtcgg cgcgggccgc gcccgccagc
     8761 agcagggtca tcaccagcgc cgccgtcgcg cgcatcgcgt cgatcatgcc tgcaccagcg
     8821 ggcccggatt tttcacgtag cggttcttga tggcgtccgc ggtccagtag gcaagggcgc
     8881 ccaccggacc ggtcgggttg tacgatgaat tgtgcgggaa cacgttggcg ccgaccacga
     8941 acagattgtg gcagtcccaa ctctgcagat atttgttgag cgcgctgtcg cgcggattgg
     9001 tgcccatgat ggtgccgccg gtattgtgcg tgctttggta gggcaccacg gtccacggat
     9061 cggtgcgcgc ggacgcgggg ttgagtcgcg tgggattcat cgatttcgcg atgtcgttga
     9121 tcagccctgc agcgtggcgg cccatcttgt gttcgttgct ctggtaatcg aacgtcatgc
     9181 gcatcagcgg ctgaccgaac gcgttgcgat aggtcgagtc gaggtcgtag caattgtagc
     9241 ggttggccat gacgctgccg ctggcactga tcaccatcgc ggtctggtac catttggccg
     9301 ttgcccgttt ccattcgctg ccccatagcg gcgtgcccga cggcaccggg cgatagccga
     9361 tcggcagcgg ggtattaaag ccgccggaca cgttatagcc gccgacgaaa tcatgcggcg
     9421 cgcggtcgaa cgcccagttg gtgttgaagt cgtcgatggt ggcgttggag ccgccggcgt
     9481 tcatgaacgg gttgaaatat ttgccatcga agaacagcgt cgcggcggcg ccggtctgat
     9541 agcaataatt cttgccgatc acgcccttct gagcgaccgg atcgtagggg gttccgatgc
     9601 ccgacagcag catcaggtgc acgttgttga tggcataggc gcatagcagc acgatggttg
     9661 ccggctgctc gaattcctcg ccgttgagca cattggtgta gctgacgccg gtgacgcgcc
     9721 ggccgtcgga atccttgagc accttggtga cccaggaatg cgtgcgcagc tcaaaattcg
     9781 gattgcgcat ggcgatcggg atcaccgtca agtgcgggct gcccttggcg ttggcttcgc
     9841 agccgaagcg ctggcaatag ccgcaatatt ggcagccgcc gaatttcgag ccgtccgggt
     9901 tggtataggg ttgcgaggca ttcgccgacg gccgcgggaa cggatggtag ccggcattgc
     9961 gcgccgcatc gctgaacatc tcgctcgcca ggatcggcgt cagcggcggc aacggatagt
    10021 cgcgtgcgcg cgacgcttcg aacggattac cgccggagag gatctgcccg ccgatattgc
    10081 cggccttgcc ggagactgcc gcggtatatt caaacttgtc atagtaaggt tcgagctcgg
    10141 cataggtgat gccccagtcc tggattgtca tgtcgtcggg aatgaatttc ttgccgtagc
    10201 gctcttcgta attggagcgg atcttgaatt ccatgtcggt ccagcgccag gtgtgaccgc
    10261 tccagtgcac gccggagccg cccaccacct cgccgggcag gaacgagccg agtcggcgca
    10321 tcggcagcgc ttcctgctgc ggattgttgc gcacggtgat ggtgtcacgc gcggtgtcct
    10381 gcatcaggtc gtggcgcacg acatagcgca actcgtcgcg gatattgggc agcgaatagt
    10441 cgttctcgct cgaacgcggg gcgccgcgct cgagcgcgac caccttcagg ccggcctcgg
    10501 tgagttcctt ggaaagaatg ccgccggtcc agcccagccc gacgatgacg acatcgacct
    10561 ctttgagttt tgtggtcatg gcgcgccctt cagctcatgt ccgaaatgcc gagcggcgtg
    10621 gtcgggaatg gcttgccgcg atatttctcc acgtgatcgc gatgcactgc gatgatgccg
    10681 ggaaagccga tgatcgccca tcccgccttg ttgcggttgc cgccgtaaat cgggtcggaa
    10741 tacatcccct cgatcacggt ctgataaagc gtcgcccaga acacccgaac cggcagtcca
    10801 ttgtcgaaaa ctattttgcc ggtggacagg ccgaccagca cctcctggcg ctgcgcgtcc
    10861 tcgatccggt cgaatgattt gccgtaagcc ttgcggcaat gcgcgttggt ggcctcgatg
    10921 ccggcgcgat aaagctgcgc aggcgtcaac ggcagttggt agccctggct cgaagtgccc
    10981 tgccgccacg gaccctgcat gtagaggcgt tcacccttgc cccaggcgcc ggcaagggcg
    11041 cgatcgatgt agacattgac gccgagatcg gtgcccttcg ggctgatctc atcggcgggc
    11101 accatgtgat ccaccaggct ctcgacgaag gcagcctcat cgagattgag gaaggcgtat
    11161 ccggcggctt gtggcgcaac ggcggttgcc gcgggcgcgg ctggctcggc ctgtgcggtt
    11221 tctggcgtta caatcgctgc acttccgcct gccgcggcgg cgccgccgac cacggcggtc
    11281 ttgagaaagt cgcgccggtc gagcttttgc tcggtcatga tcgcctccct gcctccggga
    11341 tttgaaatcc gcaaggtcat gtttttggga gagagcatgc tgtttttggc tcaacccggc
    11401 aagacaggat tgagatggct acgatgtcgc cggcggataa cgcccgacgg tttgtgcacc
    11461 gcaagagtgc tcgacgttca tcggcagccc aggtttccga tggcgttggc ggaaaggttc
    11521 ttggtgatgc agctggcctg gtcttcgcaa ttctgagggc cccgaccgtc ttgcgtactc
    11581 agcagggctt ccggatggcc tgccgggacg agcccgccgt gatcgtgacc gactatttca
    11641 tgccgaatgg caatgcccaa tatttgctga cgaggttgcg caccacggcg gcgaccgcga
    11701 acattccggt tattgttctt tccggacaac agctcaatga catgacggta caaagcctgg
    11761 ggcgagagat ttgcggccat ccgggtgcga cgcaaatcct caggaaatcc caggacacct
    11821 acgcactgtt cgaaacgttg aaaaaattct gtggcttcga acgggaaccc gtcaacttgg
    11881 tctgagaaaa cggcgtccgc gattatcgga gcgacacccc cgtcgccgtt tcgagctcgg
    11941 cgatggcttg cgatgcgttc agcaccttga tggtggtcat gcccatttcg cgcgccggtt
    12001 tcagattgac gccgagatcg tcgaggtaaa cgcaagcctt ggggtcgaca cccagcttct
    12061 cggccatcat ccgatagatg cgggggtctg gcttcctgag accgatcttg gccgactcga
    12121 tgacatgatc gaacagcgcc atcacttccg cgacgtagag cgatcggccg ccgaggcttc
    12181 cgatggcgtt ggccggaaga ttgttggtga tgcacccggt cttgaactcc gtcccgatac
    12241 gccggagcgc ctcgaccatc tcaggccgca aatctcccga cagaagcggc aagacatcct
    12301 tgccccgaac ctccgcgccc agcgcaagcg attcggcggc gaacagctga tcgaaggttt
    12361 cgatgtccac ttcggcgcgt tcgaatttcg cccaggcatt ttcccaatga ttatttgcgt
    12421 tggtgcgccg gatgatgtcg gcgggcagcc cgcgctcggt ttcgaaacgt gcaaacgcct
    12481 cgaacggcga cgtcgtaagc acgccgccaa aatcccagat cactgcctcg agcatcattt
    12541 cccgccaatg tgagcgccgt tcgtggaaac ggctagcacg gcggccttgc gggggccagc
    12601 gtcgatcgcc ccttggcgaa aaccgtggtg cgggttattt ctgctcggat gaagatcttg
    12661 cttctgacgg ttgcgggcct ggcgttgatc ctctgcgcgc ccgcctatgc gatcgtcggc
    12721 ggtggggtgc cgtctgccga tggtatcggg cgctcggtcg tgaccatcgt gggctcgcgc
    12781 ggcaactttt gcagcggcgc cctgatcgct ccgaagctgg tgttgacggt ggcgcattgc
    12841 gtgcagcccg gcgccaccta caagattgtc gagtacggcg ccgataagca gccccagctg
    12901 catgacgtga aaggcgtggc cattcacccg tcattcaatc tgcaggcgat gatggcgcat
    12961 cgcgccaccg ccgatgtcgc gttgctgcag ctcgaagcgt cgcccaaggg aaaaaatgca
    13021 tcgctgctcg gcgtgccgca ggcgccgatg gtcgccggca gccggttcac gatcgccgga
    13081 atcggcgtaa ccgcccgcgg cgaaggcaaa agcggcggga ccattcgcgt cgccggcctg
    13141 gtcgcgaccg gccggccggg aagcttgcag attcgcctcg tcgatcccgt cggacagggc
    13201 gcgcgcgacg ggcttggcgc ctgcaccggg gattccggag gcccggtgtt tgaggatcgc
    13261 cagatcggcc ctgccattgt cggcgtcatc agttggtcga ccggaccaaa cggcgccgcg
    13321 ggctgcggcg gcctgaccgg cgtcacgccg ctcacacttt accgagactg gattgtgcag
    13381 accgcccgcc agtggggcga ggcgctgtag gcacgaacgt tcaatgccgc ttttggttcg
    13441 gcttccacgg cggcggcgga ttctctccgc tgaccgcctg cggatcgaat ttgcgcgtca
    13501 cttttatggc ggcctggctg atatcgagca gggagcggcg gccctcctcc atgaaggcgc
    13561 ccgaacgctt attgaagctc tcggccagtt cgcgaatggt cctcacttta tcatacagtt
    13621 cgtcggcgct tccgcttccc aggcccggca cgaggttatc gatctttgcc atggcgccgt
    13681 cgaagctctc gaaagcgccg tcagccttgc gaatgatgct gtcgatcgcg tcgcccttgc
    13741 tcgccaacgt cgccgtatag gtctcgaaat ttgatagcgt atctttcagc atcgcctggt
    13801 taccgacgag gatccggtcg acgctgtgca gtgtatcgcg gatcgactgc acctcgctga
    13861 ggtcggccgt cagggtcgga atgccatcct tatccagcgg taccggaggt gcggcggccg
    13921 cgccgccggt cagcgatatc gccgccacgc ccgtgagccc ctggaattcc aggccgacca
    13981 ccgtgtcctt gcggatcggc gcgctgttgt ccaccatggc gagggccacc accttgcgag
    14041 ggttgtcgag cttgagcgac cttatttcgc cgacctgaac gccgtcgaag ttcacgttgc
    14101 cgccttggcg caggccgctg gcggaacctt cgaaaacgat gcgcaacgga ccgcgctgcg
    14161 ctatgccatg gattttctgg aacccgagaa aaccgacgaa cgccgcggcg atggccgcaa
    14221 gcgtcgtgga tccgatcatc aggttgctcg ccgaagcttt catcctaaaa tccggctgcg
    14281 atgcactttg ccattgccgg gttgtaccgg caaaaccaac ctggcgaaag gccgatgccg
    14341 gcggcgccac gcttggcggc gcttcttgac agagccaaag gccggggtca ccttttgatc
    14401 gaactctgct gcgcgccttg caacgaaaaa caagaatgtt ggaggaaact gtgccgaaat
    14461 tgctgagcac ggatgaagtc gccgattatc gcgaccgtgg ctaccacttc ccgatcgatg
    14521 cgctgagcgc tggcgaagtg accaaatttc gtcgcaggag attgccgtcg atgtcgacga
    14581 agagaaggcc gtcttcgtcg aactgaagcc cggccaggcc tcacttcatc acgtgctgct
    14641 gttccacggc tcggagccga accgttccga tgaccggcgc atcggtctcg ccatccgcta
    14701 catcccgacc catctgaagc aggcggtcgg ccagaaggat tgggcgacgc tcgtgcgcgg
    14761 caaggatagt tacggccact tccagccgga atatgtgccg aagcgcgacc tcgagccgga
    14821 ggcgctggcc ttccacaagg cggtgtcgga agagcaggtg cgcgtgctct atcgcggcac
    14881 cggcaagacc gcctatcgcg cgtgacgccg atgcaacgca agttttccgt cgggggcagc
    14941 ccaggacgat agatcaatca agccacatcc gatcaagatc ccgcggatcc ggacactacc
    15001 cgcagtacgg ggtcgaaagc cattgcgtct ggtggatcat cctgctcggg cgtcgcggct
    15061 tgtcgcacca ggggaaccag caagcgacag atcagacctt cggaacgcca gtcgaataac
    15121 gcctttccgc cgagctgtga ctcgatactg gcgatcacgc ttcgggttcc gaatcctctc
    15181 gacgtcggct tgcgcaccat cgggccgccg gtctcttccc atgcaagcat gaggaaatcg
    15241 ccctgcattt cccatccgat ggacaggcgc cctgacaacg tggagagcgc accatatttc
    15301 gccgaattgg tgacgagttc gtgcaaagcc agcgccaatg tttgcgccgt cgcgggctgc
    15361 aactgaac
//
LOCUS       DATNOI010000174        12240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2191479,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000174 DATNOI010000000
VERSION     DATNOI010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2191479"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VN968_06745"
     CDS             <1..192
                     /locus_tag="VN968_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HXL28970.1"
                     /translation="CHLDHFQGDGTVPRLAGLSREYLTKTMADFRTRARGNNPGMSDL
                     MLAAAPDDLAALAEYLSGL"
     gene            complement(200..1075)
                     /locus_tag="VN968_06750"
     CDS             complement(200..1075)
                     /locus_tag="VN968_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXL28971.1"
                     /translation="MSAAAVARAAESEPCRDVRLRFETIKPQISAIEVSLTLFSAVDK
                     NCLDLATELLDHGASVDARDRLGARPLSHAARIGHLGMVDLLLAHGAPIDARNLAGAT
                     ALYFAAEGGHTPVAQRLIERGADVKLAGRSRISPVAAAAYAGNDAIVEALLAAGADAR
                     APDETGKPPIVYAAAGAQLDIVKRLLAQNIDINARYPNDLTLLMWASGPDEKAPEAQA
                     VKVVSYLLDAGAHIDDRDDRGRTALMIAAEGGHAEIANLLTGRGADASLKDKAGKRAA
                     DLTVLSSLRERLTRP"
     gene            complement(1235..3190)
                     /locus_tag="VN968_06755"
     CDS             complement(1235..3190)
                     /locus_tag="VN968_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28972.1"
                     /translation="MDWPALRIQVPGGKPNLDIVLSAAALGRAAPELGDGAIHGTFIR
                     TVAGSPANDLSCATSVRSRQATRWRSAGAKRCAVKRACGAHRKGAARQEVDGRTADRQ
                     LQGADRQAGEQAAARYLPPDSDGVMRALRPTRCGGEQTSLGRPRRMFVRGIRQPGLHF
                     GACLTLIFLLAATCPGALSDELSVTPAKMARIGTIDQRFQSYNVEMVEVTGGRFWRPY
                     GPNTSDALSDLYAYRPPIDLTNARLRKLASALAPAYLRVSGTWANATHFADADVTPSA
                     PPAGFNGVLTRQQWRGVIDFSHAVGTRIVTSFAISPGTRDAAGVWAPDQARRLLAYTR
                     SVGGSIAAAEFMNEPDLAAIGAAPVAYDAAEYGRDFKLFHSFVKEEFPEIMVLGPGTI
                     GDTAFASELVATSAPGGVDAVSYHHYGALSERCNGSAAPEAALSEAWLSRTDQTLSFY
                     RLLRDRFEPGKPIWLTETAEAACGGNRWAATFLDTFRYLDQLGRLAKAGVQVVMHNTL
                     AASDYGLLDERTLAPRPNYWGALLWRQLMGTTVLDSGVPIQAGFHVYAHCQREAPDGV
                     TLLVINTGRDTAHSLTLPDASERFTLDAASLQDRTVRLNGQPLELDAKGDLPGIAGVR
                     TAAGLLTFAPATITFLSIPATGNSACR"
     gene            complement(3267..3503)
                     /locus_tag="VN968_06760"
     CDS             complement(3267..3503)
                     /locus_tag="VN968_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28973.1"
                     /translation="MGSHVYNVVKAPIGWALFCDRERIGGFGSSEAALEAATAFGANA
                     LRDGHSIQINVPGPELREIEGFESWPARWDVDLK"
     gene            3767..4174
                     /locus_tag="VN968_06765"
     CDS             3767..4174
                     /locus_tag="VN968_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HXL28974.1"
                     /translation="MISRRSVLLTLAITTLSVASVPALAGSSRADLVKMFDTDNDGTL
                     DLAEVKKAASDLFTKLDRDHDGTLDKRELAGRLSAKELAAADSDHDGTLTADEYLAVV
                     EQRFNAANPDKDGTLDAKELNSAAGRALLRLMK"
     gene            4324..4404
                     /gene="pqqA"
                     /locus_tag="VN968_06770"
     CDS             4324..4404
                     /gene="pqqA"
                     /locus_tag="VN968_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HXL28975.1"
                     /translation="MAWKAPKIVEVPVGMEINMYACAARK"
     gene            complement(4626..4934)
                     /locus_tag="VN968_06775"
     CDS             complement(4626..4934)
                     /locus_tag="VN968_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HXL28976.1"
                     /translation="MAKVVVLYKNPKNAEAFDKHYSSVHIPLAKKIPGLKKYDISMGA
                     VGAPTGPSGIHLVATLYFDSADALKAGLASAEGKAAAGDLANFADGGAELYFFDTKEV
                     "
     gene            5103..5969
                     /locus_tag="VN968_06780"
     CDS             5103..5969
                     /locus_tag="VN968_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28977.1"
                     /translation="MPIDAKAFRTAIVCLSALLANGVHAEGIDTEHLFGFMIGTDVGS
                     TGEREFQSQTTGRFSKSGGRYQAINQELELEFVPARNFRIEMGTSFASHDINGVPDFE
                     DRRQLAWQGVSVDFRYRVLDRDTAPLGLTFDVETHADRFDESTAAVVRNCGMGLALAF
                     DRELVPDFAVAALNLIYQPEWTRFVGTGAAEQESTVGAAFAVMAQMRPNFFVGGETRY
                     LRKYEGIGLQEFAGQGLFVGPTAYFKLSESSRLTLAWSFQAWGRSARSMPALDLIDFE
                     RHQARLVFGVNF"
     gene            6098..6334
                     /locus_tag="VN968_06785"
     CDS             6098..6334
                     /locus_tag="VN968_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28978.1"
                     /translation="MNPVDLIVTICAVLSPITCEETHLVFSWSGSLQQCAMGAPPYIA
                     QWVGEHPKWNAVKWRCEYPHAHDKADASGATPAG"
     gene            complement(6363..6710)
                     /locus_tag="VN968_06790"
     CDS             complement(6363..6710)
                     /locus_tag="VN968_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28979.1"
                     /translation="MSRVLFATAVFLLAAPGQLAAQGKGIRFWNLTTATVSSLHLSPA
                     GKNIWGANQTLNDKDKEVDHDERLRITGVEPGRYDAKVGYRDSKQCFVRDIEIKADAV
                     FSIADKDLKDCNK"
     gene            complement(6804..7514)
                     /locus_tag="VN968_06795"
     CDS             complement(6804..7514)
                     /locus_tag="VN968_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL28980.1"
                     /translation="MQLEVDIASKAFTNAAGERFDVLADLNFTLGAGEVGVFVGPSGC
                     GKSTMLKILAGLDHDYQGRVRRPNDARIGMVFQEPRLLPWRSVEENVRLAAPRADEAT
                     LSALFDLLELTAHRSHFPGELSLGLARRVALARAFAVEPDFLILDEPLASLDDALAAR
                     LRDQIAALVDSRAVMTLLVTHSVDDAVRLGDRLFLLSPRPARILGVVPIRTPRGARGA
                     AEISDIKAKLARFSLASI"
     gene            complement(7504..8241)
                     /locus_tag="VN968_06800"
     CDS             complement(7504..8241)
                     /locus_tag="VN968_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL28981.1"
                     /translation="MLRLLSFAVFLAVWWGAALLAGAGKLPPPPAVLAAAVAEASSGA
                     LFLNLGVTLARVALAFTLAMTLGAAIGYLMGRVRLANRLGDPWLILLLNLPALVVIVL
                     AYIWAGLTEVAAIAAIAINKLPTAVVTVREGTRALDAALDEMATVFAIPRGKAFRHVI
                     LPQLAPYIAAAARSGLSLVWKIVLVAELLGRPNGVGFEIGVAFQLFDIPLLLAYSLSF
                     VAVVLAIETMLVQPFETRLSRWRPRAA"
     gene            complement(8243..9223)
                     /locus_tag="VN968_06805"
     CDS             complement(8243..9223)
                     /locus_tag="VN968_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL28982.1"
                     /translation="MKRFTRILLATAVFATLHVVPSRAAETIRLAVQKTGTFAWELAV
                     IRAHGLDRQADLTIEVSELASPEAGKVALRSGGADVIVSDWLWVSRERGLGAKLTFYP
                     YSSALGAVMVPDSSSIRTLADLKGRKLAVAGGPIDKSWLLLRASMKQEGIDLKSESTI
                     LYGAPPLLAAKTLSGEMDATINYWNFCAALEAKGLRRLIGIEDLLPKLGATGRTSMIG
                     YVFDEGWGSANRDTVASFIAVTREAKEILASSDAEWEKIAPLTGAPDAATLHAYRDRY
                     REGIPRRPIADEEADARVLYRVLAAIGGRELVGPGAELDAGTFYRAISGD"
     gene            9371..9712
                     /locus_tag="VN968_06810"
     CDS             9371..9712
                     /locus_tag="VN968_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28983.1"
                     /translation="MRCNVCALIALVATAVVARADEVNDYPTVARSEYVFGCLKANGE
                     TRQAIEQCSCSIDVIASLLPYDRYVTAETVLSMAQVRGNLGTQFRSSEQATSALNDLR
                     RAQAEAEVRCF"
     gene            complement(9768..10550)
                     /locus_tag="VN968_06815"
     CDS             complement(9768..10550)
                     /locus_tag="VN968_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HXL28984.1"
                     /translation="MQFGVRLAPAATSETYDPWPGLVQDIFNNRAMNDGAGLIAIEMP
                     YRAEDAAIVPVTLRATPPPGDSRRVLAITLVIDQNPSPMAARFELGPDASVSEISTRV
                     RVNNYTDVHAVAELSDGQLYMTKTYVKASGGCSAPAAKNADDAKGKLGQMRFRQFAKP
                     GEGPTSGAREAQIMIGHPNNSGLQMDQLTQLYIPPFFVNELRVWQDDSLVLAMESGIS
                     ISEDPNIRFTYVPNGAKRFRVEAKDTDGHVFQNEWKVDSSGI"
     gene            complement(10659..11585)
                     /locus_tag="VN968_06820"
     CDS             complement(10659..11585)
                     /locus_tag="VN968_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HXL28985.1"
                     /translation="MIQRAAIFFAFAMLGASTLSQAQQSPLAVSEIAPGIFVHTGLTA
                     LMTPENEGAIANIGFIVGDDAVAVIDTGGSVREGRQLLAAIRARTEKPIRYVINTHGH
                     PDHVFGNAAFSSGGPTFVGHRNLPQALTTRGPFYIDAFRRIVGDELMGEVRIMLPTLL
                     VDGTLELDLGHRSLMLRAWPTAHSDNDLTVLDEQTATLFAGDLVFLGHLPVLDGSIRG
                     WLKVIDELSAVAAHRVVPGHGQVREWAGALVDERRYLEKLAADVRGLIASGKPITVAA
                     DSAAASERSQWELFDDYNARNATAAFSEIEWE"
     gene            11914..>12240
                     /locus_tag="VN968_06825"
     CDS             11914..>12240
                     /locus_tag="VN968_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXL28986.1"
                     /translation="MRKVLFATCLGAMATFAGAAAANDELSKMSQDPKGWVMPAGSYD
                     NQRYSKLKQINAANVGKLQVAWTFSTGVLRGHEGGPLIVGNTMYVHTPFPNKVYALDL
                     SDENKIV"
BASE COUNT         2379 a         3724 c         3799 g         2338 t
ORIGIN      
        1 tgccatctcg atcattttca gggcgacggc acggtgcctc gcctggcggg cctgagcagg
       61 gagtacctga caaaaacaat ggccgacttc cgcacccggg cgcgcggcaa caatcccggc
      121 atgtccgact tgatgcttgc cgccgcgccg gacgacctcg ctgcgctggc ggaatatctg
      181 tcgggtctct gaagcgggtt cagggccgcg tcaatcgttc gcgcagtgaa gacagcacgg
      241 tgaggtccgc ggcgcgtttt ccggccttat ccttgagcga cgcatccgct cctcgccccg
      301 tcaatagatt ggcgatctcc gcatggccgc cttcggccgc aatcatcagc gcggtgcggc
      361 cgcggtcgtc ccgatcatcg atatgggcgc ctgcgtccag cagatatgaa acaaccttga
      421 ccgcctgcgc ttccggggct ttttcatccg gacccgatgc ccacatcagc aaggtgagat
      481 cattgggata gcgggcgttg atatcgatat tttgcgccag taaccgcttc acgatgtcga
      541 gctgagcgcc ggccgccgca tagacgatcg gcggcttgcc ggtttcgtcg ggcgctcgcg
      601 cgtcggctcc ggcagcgagc agcgcttcga cgatcgcatc gttgccggca taggcggcgg
      661 ccgcgaccgg agagatcctg ctgcgcccgg cgagtttgac gtcagcgccg cgttcgatca
      721 gccgctgcgc gaccggcgtg tggccgcctt cggcggcaaa atagagcgcg gtggcgccgg
      781 cgagattgcg ggcatcgatc ggagcgccgt gcgccagaag aaggtcaacc atcccgagat
      841 gacctattct cgcggcgtga ctgaggggcc gggcgccaag ccggtcgcgc gcgtcgaccg
      901 atgcgccgtg gtcgagcagt tcggttgcga gatcgaggca gttcttgtcg accgcggaaa
      961 acagcgtcaa cgacacttcg atcgcgctga tctgcggttt gatcgtttca aaccgaagcc
     1021 ggacatcccg gcaaggctcg gattccgcgg cgcgcgcgac ggcggctgca ctcaacgcgg
     1081 caacaatcga acatagcaaa gcgatttgac ggtacacgcg gtctctcccg gtcaggcatg
     1141 tcggctgagg gtggcatggc ggctgcgcaa ttccaagagt gcgaacaagt ggtcgtcgcg
     1201 gcatccgcga gctcgacatc cgcttgatgg tccctcatcg gcaggcgctg tttccggttg
     1261 cgggaatgga cagaaaagta atcgtcgccg gcgcaaacgt cagcaggccg gcggcggtcc
     1321 gaacacctgc gatgcctggc agatcaccct tcgcgtccag ttccaatggt tggccgttca
     1381 gacgaacagt tctgtcctgc aagctcgccg catccagggt aaaacgctcc gaggcgtcgg
     1441 gaagcgtcag cgagtgtgcg gtgtcgcggc ccgtgttgat caccaaaaga gttacaccat
     1501 caggggcttc gcgctggcaa tgcgcataga catgaaagcc cgcttgtatc ggcacaccgg
     1561 agtcgagcac ggtggtcccc atcaattgcc gccacagcag cgcgccccag taatttggtc
     1621 ggggtgccag cgtcctctcg tcgagcaggc cgtaatcgct cgccgccagg gtgttgtgca
     1681 tcacgacctg aaccccggcc tttgccagcc gcccaagctg atcaaggtat ctgaacgtgt
     1741 ccagaaaggt tgcggcccac cggttgccgc cgcacgctgc ctccgcggtt tcggtcagcc
     1801 aaatcggctt gccgggttcg aatcggtccc gaagcaaccg gtagaaactc agcgtctgat
     1861 ccgtccggga gagccacgct tcggaaagcg cggcctccgg cgcggccgaa ccgttgcacc
     1921 gctccgaaag cgctccatag tgatgatagg aaaccgcgtc tacgccgccg ggggcggatg
     1981 tggcgaccag ctccgaagcg aaggctgtgt caccgattgt gccgggaccg agcaccatga
     2041 tctccggaaa ttcttccttc acaaacgaat ggaaaagctt gaagtcacgt ccgtattcgg
     2101 ccgcgtcata ggcaacgggt gcagcgccga tcgcggcgag gtccggctcg ttcatgaact
     2161 ccgcggcggc gatcgagcct ccaaccgagc gggtataggc gagcaggcgg cgcgcctggt
     2221 ccggtgccca gacgccggcg gcatcgcggg tgcccgggct gatcgcgaat gacgtcacga
     2281 tccgcgtccc aaccgcatgc gagaaatcga tcacgcctcg ccattgctgg cgggtcagaa
     2341 ctccgttgaa tccggccggc ggcgcggatg gcgtgacatc cgcatccgca aaatgggtgg
     2401 cgttcgccca ggtcccgctg acacgcagat aggcgggcgc caatgccgag gccagctttc
     2461 gcaaccgggc gtttgtcaga tcgatcggcg gccggtatgc gtataaatcg gaaagtgcat
     2521 ccgaagtgtt cggtccatag ggccgccaaa atcttccgcc agtcacttcg accatttcga
     2581 cgttatagga ttggaaacgt tggtcgatcg tgccgatgcg ggccatcttc gcaggtgtca
     2641 cggacagttc gtcggagaga gcgccggggc aagtagcggc gaggagaaat atcaaggtca
     2701 ggcaagcccc aaagtgaaga ccaggctgtc ggatcccacg cacgaacatt ctccgtggcc
     2761 gaccgagcga agtctgttcg ccaccacaac gcgttggccg gagcgccctc atcaccccgt
     2821 cggaatctgg gggcaggtac cgggccgcgg cttgctcccc cgcttgtcga tcggcacctt
     2881 gcaattgtcg atccgctgtt cgtccatcca cttcttgccg agccgctcct ttccggtgag
     2941 caccgcaggc tcgtttgacg gcgcagcgtt tggctccggc cgatcgccag cgggtagcct
     3001 gccttgagcg gacgctggta gcgcaagaca gatcgttagc aggggaacca gcaacggtcc
     3061 ggataaaggt cccatggata gctccatctc cgagttcggg tgcggcccga ccaagggcag
     3121 cggccgatag aactatatcg agatttggct ttccacccgg aacctggatg cggagggcag
     3181 gccaatccac cgcggcgcgg caagtccggg cgcgccgggc ctgccgggca ggtggcggat
     3241 agaggctgtt gagggacgcc cgttggctat ttcagatcga cgtcccaccg cgccggccac
     3301 gattcaaagc cttcgatttc gcgcagctcc ggtcccggca cgttgatttg aatgctgtgg
     3361 ccgtctcgaa gcgcatttgc gccgaatgcg gtcgccgcct ccagcgccgc ctcactcgaa
     3421 ccaaatccgc cgatgcgttc gcggtcgcaa aacagcgccc agccgatcgg agctttcacg
     3481 acattataaa cgtgacttcc catgggtcac ctcactcagg aacagtatgc atacttaaat
     3541 ggtaaccgtt cgacggcacc accacatcgc gcctgttgag ccaatgcgaa gatcggcgac
     3601 cccgcgcgcg tcggttcggg ttggaacttc ggggcgggtt tcccttcaca ttgacacgat
     3661 tgcaatcaac gatagcctat cgcgacgctg cggcgatggc gagctaacct cgcacctcgt
     3721 gccggatttt gattaaagaa aaacgggaga tcaaccgatg atgatgatga tatcgcgccg
     3781 atcggttctg ttgacattgg ctatcactac gttgtccgtt gcaagcgtgc cggccctggc
     3841 gggctcctcc cgggccgatc tggtcaaaat gttcgacacg gataacgacg gaacgctcga
     3901 ccttgcggag gtgaagaagg cggcgtccga tttgttcacc aaactggacc gcgatcacga
     3961 tggcacgctc gacaagcgtg aattggcggg gagattgagc gccaaggaat tggccgctgc
     4021 cgattccgac cacgatggca cgctgacggc agatgaatat cttgccgtcg tggagcaacg
     4081 cttcaatgcg gcaaatccgg acaaggacgg aaccctggat gccaaggagc taaacagcgc
     4141 cgctggacgg gcgctgttgc gattaatgaa gtagttcgct cctagtcatt gatgtagttc
     4201 gctcctggtc aatcgcagcc cccgtgcaac gcaaaaccat aatgtgaaca cttgcgctat
     4261 attcgaggtg gccttaacct tcgctctggc gccacatcgc gcctaagaac ctgggagaaa
     4321 cgtatggcct ggaaagctcc aaagattgtc gaagtgccgg taggcatgga aatcaatatg
     4381 tacgcctgcg cggcgcgcaa atagaccgca tcgaggcgat ctgcccggct ccagaagcaa
     4441 cgtcttgctt cgagccgcgg caggcgtcca tcgagccgga ttcgacgccc cggtttgacg
     4501 ggcccgcttt taaagactgg cgcttggact gcagtgtcca tccgaccgcc catccggggt
     4561 cgacggcccc ggcctcccca aatactcttc tcggttaggg ttggccgagg gctggccgag
     4621 gggtctcaga cttctttcgt atcgaaaaag taaagctccg cgccgccatc cgcgaagttg
     4681 gcaaggtccc ccgccgctgc cttgccttcg gccgacgcca agccggcttt aagcgcatca
     4741 gccgagtcaa aatagagcgt agccacaagg tgaatcccgc tcggcccggt gggcgctccg
     4801 accgcgccca tgctgatgtc gtatttcttc agaccgggaa ttttctttgc gagcggaatg
     4861 tgaaccgaag aataatgctt gtcgaatgct tcggcgtttt tcggattttt gtaaaggacg
     4921 acaacttttg ccattcggtc tctcccagcg ccggtgattt gttacgatac gtgcaacccg
     4981 gtcgaacatg gagccaaccc ggtattgtcc cgcggatcaa gtatttccgg cgtctcgcca
     5041 gcaatcattg tgataggctc ctgtcggtca gccatgctgt cgcttgccac ttcggaatgt
     5101 gaatgcctat cgatgcaaaa gcgtttcgaa ccgcaatcgt gtgtctttcg gcgctcttgg
     5161 cgaacggcgt gcacgccgaa ggcatcgata ccgagcatct gtttggcttc atgatcggca
     5221 ccgatgtcgg cagcaccggc gaacgcgaat tccagagtca gacgaccggg cgattttcca
     5281 aaagcggcgg ccgttatcag gcaatcaatc aggaactcga actggaattc gtacccgcca
     5341 ggaattttcg catcgagatg gggaccagct ttgcgtccca tgatatcaat ggcgttccgg
     5401 atttcgagga tcgccgccaa ttggcctggc agggcgtttc cgtggatttc cgctaccggg
     5461 tcctggaccg ggatacggct ccgctgggtc tgaccttcga cgtggaaacc catgctgacc
     5521 gcttcgatga gagcacggcg gcggttgtcc ggaattgcgg catgggattg gctctggcgt
     5581 tcgatcgcga actggttcca gactttgccg tcgcggcgct caatctgatt tatcagccgg
     5641 aatggacgcg ctttgtcggt accggagccg cggagcagga atccaccgta ggtgctgcgt
     5701 tcgccgtgat ggcacagatg cgcccgaatt tctttgtcgg cggtgaaaca cgctatctgc
     5761 ggaaatatga gggcatcggc ctgcaggagt tcgccggcca aggtttgttc gtcggtccga
     5821 ccgcttattt caagctgtcg gagagttcgc ggctgacgtt agcctggagc tttcaggcgt
     5881 gggggcgttc agcccgatca atgcctgccc tcgatctcat cgatttcgag cgccaccagg
     5941 cgcggctggt gttcggcgtc aatttctagg ctgaaggccg ccggaccggt gcgatggtat
     6001 gatccggagg cggattcttc acggtgtctt gaaacctcac accgccggtt tcgtagctat
     6061 ttgtgctatc gatttgagga ttcggggggt gtcaggcatg aatcctgtag acctaatcgt
     6121 gacgatatgt gccgtgctgt cgccgattac ctgcgaagag acccacctgg ttttttcctg
     6181 gagcggttcg ctgcagcaat gtgccatggg tgcgccgccc tacatcgcac aatgggtggg
     6241 cgagcacccg aaatggaacg cggtcaagtg gcgctgcgaa tatccgcacg cccatgacaa
     6301 ggcggatgca agcggggcga cgcccgcggg ctgatcgatg agtcggcgac ttcgcgatcg
     6361 cgctacttgt tgcagtcctt caaatccttg tcggcgatcg aaaacaccgc atcggccttg
     6421 atctcgatat cccgcacaaa acattgcttt gagtcgcgat agccgacctt ggcatcgtaa
     6481 cgcccaggct ccacgccggt gatgcgcagg cgctcgtcgt ggtcgacctc cttgtccttg
     6541 tcgttcaggg tctggttggc gccccagatg ttcttgccgg cgggcgaaag gtgtaagctg
     6601 gaaacggtcg cggtggtcag gttccagaac cggatgccct tgccctgcgc cgcaagctgg
     6661 ccgggcgcgg cgagcaggaa cacggcggtg gcaaacagaa cgcgcgacat gatgacctcc
     6721 ctgtgcgttt cccattgaac tatcatggcg cggaacgatg acgccagccg tggcatggcc
     6781 ggataccgat ctctgatgaa gctttagata cttgccaggc taaacctcgc cagcttcgcc
     6841 ttgatgtcgc tgatttcggc ggcgccgcgc gccccgcgcg gtgtgcgaat tggcacgacg
     6901 ccaagaatcc gggccgggcg cggtgacagg aggaacagcc ggtcgccaag acgaaccgca
     6961 tcgtccacgc tgtgggtgac gagcagcgtc ataacggcgc gactgtccac cagcgcggcg
     7021 atttggtcgc gcaggcgggc ggcgagggcg tcgtcgagcg acgccagcgg ttcgtcgaga
     7081 atgaggaagt cgggctcgac cgcgaacgcg cgggcgagcg cgacgcgccg ggcgaggccg
     7141 agcgatagct cgccgggaaa atggctgcga tgcgcggtca attccagcaa gtcgaatagc
     7201 gcggaaagcg tggcttcatc cgcccgcggt gcggcaagcc gcacgttttc ttcgaccgac
     7261 cgccagggca atagccgcgg ctcttgaaac accatgccga ttcgcgcgtc gttggggcgc
     7321 ctaacgcggc cctgatagtc atgatcgagg ccggcgagga tttttagcat ggtgctcttg
     7381 ccgcagccgg atgggccaac gaacacgcca acttctccgg cgccaagcgt aaagttgaga
     7441 tcggcgagca cgtcgaaccg ctcgccggct gcgtttgtaa acgccttgct tgcgatatcg
     7501 acctcaagct gcacggggcc gccatcggga tagccgcgtt tcaaatggtt gcaccagcat
     7561 ggtttcgatg gccagcacga ccgccacgaa actcagtgaa taggccagca ggagagggat
     7621 gtcgaagagc tggaacgcca caccgatctc gaagccgacg ccgtttggac gtccgagcaa
     7681 ttcggcaacc aggacgatct tccagaccag ggaaagcccg gatcgcgccg ccgccgcgat
     7741 atagggcgcc agttgcggca atatcacgtg ccggaacgcc ttcccgcgcg ggatcgcaaa
     7801 caccgtcgcc atttcgtcca gcgccgcgtc gagcgcccgg gttccttcgc gcaccgtgac
     7861 gaccgcggtc ggtagcttgt tgatggcaat ggccgcaatc gcggcgactt ccgttaatcc
     7921 ggcccagata tacgcaagca cgatgacgac gagcgccggc aggttgagca gcaggatcag
     7981 ccagggatcg ccgagccggt tggcaagtcg tacccgaccc atcagatagc cgatggccgc
     8041 gcccagcgtc atcgccaacg tgaaggcgag ggcaacgcgt gccaatgtta cgccgagatt
     8101 gaggaacagc gcgccggaac tggcttccgc aacagctgcc gccagcacgg cgggaggcgg
     8161 cggcagcttt cccgcgcccg caagcagggc ggcgccccac cagaccgcaa gaaacacggc
     8221 gaacgacagc agacggagca tctcagtctc cggagatggc gcgatagaat gtgccggcgt
     8281 caagttctgc ccccggcccg acaagttcgc gaccgccgat cgccgccagc acgcgataaa
     8341 gaacgcgcgc gtcggcttct tcgtcggcga tcgggcggcg gggaatacct tcccgatagc
     8401 ggtcgcggta ggcgtgcaag gttgcggcgt cgggggcacc ggtcagcggc gcgatctttt
     8461 cccactccgc atccgagctc gccaaaatct cctttgcctc gcgggtcacc gcgataaagc
     8521 tggcaacggt atcgcggttc gcgctgcccc acccttcgtc gaagacgtag ccgatcatcg
     8581 acgtgcggcc cgtggcgccg agcttcggca gcagatcctc gatgccaatg agacggcgga
     8641 ggcctttggc ttccagcgcg gcgcagaaat tccaataatt gatggtggca tccatttcgc
     8701 cgctgagcgt cttcgccgcc agcagcgggg gcgcgccgta taggatcgtg gactccgact
     8761 tcaggtcgat gccctcctgc ttcatcgatg cccgcaacag cagccagctt ttgtcgatcg
     8821 gaccgccggc gacggcgagc ttgcggccct tgaggtccgc caaggtccgg atcgaagatg
     8881 aatccggtac catcaccgcg ccaagcgcgc tggaatacgg atagaacgtc aatttggcgc
     8941 cgagcccgcg ctcgcgcgac acccacagcc agtcggagac gatcacatcg gcgccgccgc
     9001 tgcggagcgc gaccttgcct gcctcgggac tcgcaagctc gctcacctca atcgtaaggt
     9061 cggcctgcct gtcgaggccg tgagcccgga tcacggcaag ttcccaggcg aatgttccgg
     9121 tcttttgcac tgcaagccgt attgtttccg cagcgcggga tggaacgaca tgcagtgtgg
     9181 cgaaaacggc cgtagccagc agaatacggg tgaagcgctt cattgtgccc ttgttgatta
     9241 tcggagcccg acgcccggca ggcatctcag gttcgacaag acgtgcgatg agtggtttcc
     9301 tcgacaggct cgttttccgt atgatagcac agctcccagc aggaggaatg cgatgccatt
     9361 tgccggatcg atgcgatgta atgtctgcgc cctgatcgcg ctggtagcaa cggccgttgt
     9421 cgcacgcgcc gacgaagtca atgactatcc cacggtggcg cgatcggagt atgtgtttgg
     9481 ctgcctgaag gcgaatggcg agacgcggca ggcgatcgag caatgctcct gctcgatcga
     9541 cgtcatcgcg tccctgctgc cctacgatcg ctacgttacc gccgagaccg tgctgagcat
     9601 ggcgcaggta cggggaaatc tcggtacgca attccgctcg tccgagcagg cgacgagcgc
     9661 gctcaatgat ctccggcgcg cccaggctga agcggaagtg agatgtttct gagccgcgcc
     9721 gtgctgcgcg gccgtaccgc gccaatccgg gcggcatcgc accgcattca aatcccggaa
     9781 ctgtcgacct tccattcgtt ctggaacacg tggccgtcgg tgtccttggc ctcgacccgg
     9841 aagcgcttcg cgccgttcgg aacataggtg aatcggatat tgggatcttc ggaaatcgaa
     9901 attccgcttt ccatcgccaa gaccagactg tcgtcctgcc acacccgcaa ttcattgacg
     9961 aagaacggcg gaatatagag ctgggtgagc tggtccatct gcaggccgga attgttcggg
    10021 tggccgatca tgatctgggc ttcccgcgcg ccgctggtcg ggccctcgcc gggctttgcg
    10081 aactgcctga agcgcatctg gccgagtttg cctttcgcat cgtctgcgtt cttggcggcg
    10141 ggggccgagc atccaccgga cgccttcaca taggttttcg tcatgtagag ctggccgtcg
    10201 ctcagttcgg cgacggcgtg aacgtcggtg taattgttga cgcgcacgcg cgtcgagatt
    10261 tccgataccg atgcgtcggg tccgagttcg aaccgcgccg ccatcggcga cgggttctgg
    10321 tcgatcacca gcgtgatcgc cagcacgcgc ctgctgtcgc cgggtggcgg cgtcgcccgc
    10381 agggttacgg gaacgatcgc cgcgtcctcg gcgcggtacg gcatctcgat ggcgatcaag
    10441 ccggcaccgt cgttcatcgc acgattgttg aagatatcct ggaccaggcc gggccaggga
    10501 tcataggtct cggatgtcgc agcaggtgcg agacggacgc cgaactgcat cgcagccagg
    10561 agaccggcga tgcagagcag gcgggaaggg tgtcccaaca tggtcgcgct cctcacaacg
    10621 acggaaagca acgaacccaa tctacttcat tgagccggct attcccattc tatttccgaa
    10681 aatgcggccg ttgcgttacg cgcattgtaa tcgtcaaata gttcccattg cgaccgttcg
    10741 gaggcagcgg cgctatcggc cgcgaccgtt atcggcttgc cactggcgat cagcccgcgg
    10801 acatccgcag cgagtttttc cagatagcga cgttcgtcga caagtgcgcc tgcccactcg
    10861 cgtacctgtc catgaccggg aaccacacgg tgggcagcaa cagcgctgag ctcatcgata
    10921 actttcagcc agccgcgaat gctgccgtcg agcacgggca ggtgaccaag gaacaccaga
    10981 tcgcccgcga acagggtagc ggtttgttcg tcgagcacgg tgaggtcgtt gtcgctgtgc
    11041 gcggtcggcc acgcccgcag catgagactc ctgtgcccga gatcgagctc gagggtgcca
    11101 tccaccagca gcgtaggtag catgatgcgt acctcgccca tcaattcatc gcccacaatc
    11161 cggcgaaatg catcgatata aaacggtccg cgcgtggtca gggcttgcgg caaattcctg
    11221 tgtccgacga aagtcgggcc accgctcgag aatgccgcat ttccaaagac atgatccggg
    11281 tgaccgtgcg tgttgatgac gtatcggatc ggcttttccg tgcgcgcgcg aatcgccgcg
    11341 agcagctgcc gtccctcgcg aacactgcct ccggtatcaa tcaccgcgac cgcgtcatcg
    11401 ccaacgatga atccaatatt ggcgatggcc ccttcgttct caggtgtcat gagcgcggtc
    11461 aggcccgtat gcacgaatat ccccggcgcg atctcgctga ccgccagcgg actttgctgc
    11521 gcctgcgaga gcgttgatgc accaagcatt gcgaacgcga aaaaaatggc agcgcgctga
    11581 atcataattt cgcctccgtc gcgccacgaa gcgacgtcgg ctgcttcgcg ctctatcgct
    11641 gaaacatgtt tgcgtcgcag cacacggagc cgacgagccg ccaagatgat ttctgttgca
    11701 cgctccaatt ggcgagccta gcatttaggt gcttccggct tcaattgcac ttcgtgtctt
    11761 gttgaagttg agcgacgcgg cagaacgaaa gtgcaatggc tttcgggcat gcattcggta
    11821 tgacatgcat tcggtatcaa gagtcgacgc cgggcgcatg tgcccaccgg acaaataaaa
    11881 taaaaagctg aaaacaacat gggagggatc aatatgcgca aggtgctatt tgcaacttgt
    11941 cttggcgcta tggcgacatt cgcaggtgcc gctgctgcca atgacgaact gagcaagatg
    12001 tcgcaagacc ccaagggctg ggtgatgccg gcaggcagct acgacaatca gcgatattcg
    12061 aagctcaagc agatcaatgc cgccaatgtc ggcaagcttc aggtggcatg gacgttctcg
    12121 accggcgtgc tgcgcggtca tgaaggcgga ccgctgatcg tcggcaacac gatgtacgtc
    12181 cacacgccgt ttccaaacaa ggtctatgct cttgaccttt ccgacgagaa caagatcgtc
//
LOCUS       DATNOI010000175         8064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1769018,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000175 DATNOI010000000
VERSION     DATNOI010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1769018"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(344..1381)
                     /locus_tag="VN968_06830"
     CDS             complement(344..1381)
                     /locus_tag="VN968_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HXL28987.1"
                     /translation="MQLPPHMKVVRITEAGGPEVLSLQDEPRPEPQTGEVLVRVAAAS
                     VNRPDIQQRRGFYPPPPGASEIPGLDIAGVVDKAAPDVAWPRVGDAVCALVTGGGYAE
                     YCRVPALQCMAIPRGYDFVRAAALPEVLFTSWNNVILLARLSEGESILVQGGTSGVGM
                     AAIQIAKLLRRARVFATAGSEQKRQICRDLGAEIALDYRSDWVQPLRSATGDAGVDVI
                     LDAQAGPYTDRELQLLAFDGRLVLIASHLGERADINVRQIVRRRLTLTGSTIRPRLPE
                     YKGQIAQALVEQVWPLLEDGRMRLDVCATFPLAEVPRAHAMLDANEQIGKVVLIVDPE
                     LAGVIPHLRAA"
     gene            complement(1381..2724)
                     /locus_tag="VN968_06835"
     CDS             complement(1381..2724)
                     /locus_tag="VN968_06835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL28988.1"
                     /translation="MDIVDRDGSAALMNRIMWRIMPLLMGMMMLSVIDRSNVGYAKLQ
                     MASSLGMTETMYGLASSLFFIGYSFFEIPSALAAHRFGARVWFARILLTWGLLTVALG
                     FTMSGSVFAAVRFLVGVAEAGAYPGIIFYLTLWFPKRYQVQAVALLTIGSPLGNMFGS
                     LFGGAFLDLNGLFGLAGWQWVFIVTGAPAVILFVLILRFLPDNPLKADFLAPAEKAWL
                     ADELRRDETAAAMHTSPLRVLIDLRVWWFALVYTMITLALYGIIYWLPTVVKGFGTTG
                     TQNGLLNALPWAVAAVVLVWLPRHLREHRTVLVGMALIALCGMSAFFTSTMLTQNWMR
                     YVALAVGTPCVSLLFPCFWYLPSQIFKGAHAAAAIAAISTIGSLGGFVAQNLMPWVAH
                     WTGSALAAMAVPATSLALLAVSALIMLATWRVRPHVVFDAASMLPAAAERTQALS"
     gene            complement(2784..3758)
                     /locus_tag="VN968_06840"
     CDS             complement(2784..3758)
                     /locus_tag="VN968_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007767897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HXL28989.1"
                     /translation="MANNQVSYPDTVPLPPLSRPAEVEELLIGAVDLHCHSGPAAMPR
                     ILDHHDELLDAAAAGFRAVLYKDHFYPGMAHAIMLEKLFPELGVRLYSGIALNNASGG
                     INPHAVDHAIKLGGKSVWMPTLSAANHINVLASGNAKTFPKTSQKMLDPTPLSALDAN
                     GRLTDETKKVIDLVAEADIILAGGHLHVTEQHLVFAEAARRGVKKMMVNHPTYIVGFG
                     DSDIRQLVGLGVVMEHSICMFIKGKSEKYSADDLAHLIEVAGVDNTILSSDLGLVGSQ
                     RPVDGFRSITQMLLDLQMPRAAIKKLVSENAAAFLNLTANRAADQNAA"
     gene            complement(3770..4702)
                     /locus_tag="VN968_06845"
     CDS             complement(3770..4702)
                     /locus_tag="VN968_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL28990.1"
                     /translation="MYHSSDPRAALAAKPVGIQSNPTRFAGAEYAKFYEMPPAEEKDG
                     ARNWYARGQNFIIAYSEATKGAVLSRAEQPDEYVVLLPDPGAGAEIVWDNERITVTAH
                     SITFVPAGKSSATLLGPAKIIRMMTTKSKDLVALCSNAESYASAHPNLPAFEPWPEPT
                     AGPKIRTYDLEVAPSPGRFGRIWRGSTFMVNFLDPKNGPRDTSKMSPHHHDDFEQCSL
                     ALAGTFIHHMRWPWTPDMGQWREDTHERCDSPSIAVIPPPAIHTSQAVYEGLNLLVDV
                     FCPPRMDFSTKPGWVLNGDEYPMPPQAETNTNTN"
     gene            complement(4692..5498)
                     /locus_tag="VN968_06850"
     CDS             complement(4692..5498)
                     /locus_tag="VN968_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXL28991.1"
                     /translation="MSGEVKPHFSSFRRRLTGGQNLVGSFIKTPTTHASEIFGALGYD
                     FVVIDEEHAPFDRAVTDVVVLAARASNLASIVRVSSGDPTKILSCLDCGATGVLVPHV
                     ASVEKARAVAEVSRYHGGRRGYSGSPRAGGYGATPMWQLVEEQDASVCVIAMIEDPEA
                     LDHIGEIAAVDGIHGLFIGRGDLTVSLGAKSSAPKVQDAVKRVIAAAQQAKKPVCVMV
                     ASAAEAKDFADLGASAFIISSDQGQMRRAAAQSLTDFNKLVQTPEHTHVS"
     gene            5641..6417
                     /locus_tag="VN968_06855"
     CDS             5641..6417
                     /locus_tag="VN968_06855"
                     /inference="COORDINATES: protein motif:HMM:NF007342.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HXL28992.1"
                     /translation="MPLRSVKVEAAARTLVLLEELNRHRVASIQVLHRATGLPKSTVV
                     RLMKSLCAMGYAANDRRQGGYTVASRVKSLSNGFHGDPLVVEAARPWALAFTAQYHWP
                     VAIAVLDGASVVVRFSTIPDSPVSPFHGTINMRLSLLRRALGRAYLAFCPADERGMLL
                     DMLSRSTDPEDRIAVDRRRTQALLAVIRKQGYAERDPMVEPRSSGTIAVPIIGKGKVL
                     ATVGMTYFMSALDRRDLAQRYAPLIQTLADNISSSVASLR"
     gene            6536..7477
                     /locus_tag="VN968_06860"
     CDS             6536..7477
                     /locus_tag="VN968_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL28993.1"
                     /translation="MPTADVSPQDQALIGAPLPPFWNDHPKDAAAWKALIQTRADQII
                     KTLPGLREKFGVKSEQMTIAGVNCYMLTPDNIPEQNRDRLLVHVHGGGYVFAPAEAAT
                     REAILMAGFGKFKVISIDYRMPPDYPYPAAMDDAMAVWKEVVKTNAPNKMAIFGTSTG
                     GGMTFAMVLRARTEGLPLPAATAPGTPWSDMTKTGDTFFTNEKVDNILVSNDGWLGDA
                     AKLYANGHDLKDPQLSPVYGDLSGFPPTILTSGTRDLFLSNTVRVHRKLRQAGVAADL
                     HVFEGQSHAQYAGDPDAPETKEHFGELTAFFDKYLAK"
BASE COUNT         1629 a         2547 c         2405 g         1483 t
ORIGIN      
        1 gaagttggcg gacatgccga tggagcggct gcactcgcgc aaactcagaa acttctcgga
       61 aacggccttc cagtaggcgc accgctgttt catggcccac gctccatctt tgccaaaaga
      121 ttagtgtgtt gcgctgacca attgaacccg cggccaatag cgggcctcgt ctaggagacg
      181 aaattggggt gaaagcagaa ccagcagctg ctggtatctt cgctcccttc ctcatttcca
      241 ctgaaatgca aagcggcgct acgtccgctt tgagccgacg gcggccatcc ggcgtcgaat
      301 gcgatatttg taaatggaaa tcttctcgct catgatgact tactcatgcc gcccgcaagt
      361 gcggaatcac gccggcaagc tccgggtcca cgatgaggac aaccttgccg atctgctcgt
      421 tggcgtccag catcgcatgg gcgcggggaa cctcggccag cggaaatgtc gcgcagacat
      481 ctagtcgcat ccgcccgtct tcgagcagcg gccagacctg ctcgaccagg gcctgcgcga
      541 tttgcccctt atattccggc aatcgcggac gaatggtcga cccggtcaga gtcagcctgc
      601 gccgcacgat ctgtcgaacg ttgatgtccg cacgttcgcc gagatggctt gcgatcagca
      661 ccagacggcc gtcaaaagca agaagctgca actcacgatc ggtgtagggg ccggcctgcg
      721 catcgaggat gacgtcgaca cctgcatcgc ccgtcgccga gcgcagcggc tgcacccagt
      781 ccgacctata atcgagggcg atttccgcgc cgaggtcacg acagatctgt cgtttctgtt
      841 ccgatccggc ggtggcaaaa acacgtgccc ggcgcagcaa tttcgcaatc tggatcgccg
      901 ccatcccgac gccgctggtt ccgccctgca cgaggatgct ctcgccctcg gaaagccgtg
      961 cgagtagaat gacgttattc cagcttgtga acagaacttc cggaagcgcg gccgcacgca
     1021 cgaagtcgta acctcgcggg atcgccatgc attgcagcgc aggaacccgg caatattccg
     1081 cgtagccacc accggtcacc agcgcacaga cggcatcgcc gacccgcggc cacgcaacat
     1141 ccggcgcggc tttatcgact acgccggcga tatccagccc aggaatctcc gacgcccccg
     1201 gcggcggcgg gtaaaatccg cgccgctgct gaatgtccgg acgattgacg cttgcggcgg
     1261 ccacgcgaac cagcacttcg ccggtttgcg gctctggccg cggttcgtcc tgcaaagaca
     1321 gcacttccgg gccgccggct tcggtgatcc tgacgacctt catgtgcggc ggcaactgca
     1381 tcacgacaaa gcctgggttc gctcggcggc cgccggcagc atcgaggcag cgtcaaaaac
     1441 aacatgcggc ctcacccgcc aggtcgccag catgatcaat gcactgaccg ccagaagggc
     1501 caggcttgtt gccggcaccg ccatggcggc aagtgcgctg cctgtccagt gcgcgaccca
     1561 tggcatcagg ttctgcgcga caaatccgcc aaggctgccg atcgtgctga tcgcggcgat
     1621 cgccgccgcc gcgtgtgcgc ccttgaaaat ctgcgacggc agataccaga agcaggggaa
     1681 caacagcgac acgcaaggcg tccctaccgc cagcgcaacg tagcgcatcc aattctgcgt
     1741 cagcatcgtg gatgtgaaga aagcggacat cccgcaaagc gcaatcaagg ccatcccgac
     1801 cagaacggtc cggtgctccc gcagatgccg cggcagccac accagaacga cggccgcgac
     1861 agcccagggc aatgcattca gcaggccatt ttgagtgcct gtcgtgccga accccttcac
     1921 cactgtgggt agccaataga tgatcccgta tagcgccagc gtgatcatcg tatacaccag
     1981 cgcgaaccac caaacccgca agtcgatgag gacgcgcagc ggactggtat gcatggccgc
     2041 ggcggtttca tcacgcctca gttcgtcggc aagccaggct ttctcggcag gggcgaggaa
     2101 atcggccttg agcggattgt cgggcaggaa tctcagaatg agaacgaaaa gaataacggc
     2161 cggcgcaccg gtgacgataa agacccactg ccatccggcc agcccgaaca gaccgttgag
     2221 atccagaaac gcgccgccaa acagcgatcc gaacatgttg ccgagcggac tgccgatggt
     2281 cagaagcgcc acggcctgca cttggtagcg cttcggaaac cacagcgtca gatagaaaat
     2341 gatgcctgga taggcgccag cttccgccac gccgaccaga aaccgcaccg cagcgaacac
     2401 cgagccgctc atggtgaagc cgagcgccac cgtgagcaga ccccacgtca ggaggatgcg
     2461 ggcgaaccag acccgcgcgc cgaatctgtg cgccgccagc gcacttggta tctcgaaaaa
     2521 cgaatagccg atgaagaaca gcgatgacgc aagaccatac atggtctcgg tcattccgag
     2581 gctgctcgcc atctgcagct tcgcataacc gacgttcgaa cggtcgatga ccgagagcat
     2641 catcatgccc atcagcaagg gcatgatccg ccacatgatg cgattcatga gcgcagcgct
     2701 gccgtcgcga tccacgatat ccatctttcc tcccgacccg tccagcgggc gtgtgcttgc
     2761 agcggccgtc taggctcttg ttttcaggct gcgttttgat cggctgcgcg gtttgccgtc
     2821 agattcagga acgcggccgc attttcactt acgagcttct tgattgccgc acgcggcatc
     2881 tgcagatcca gcagcatctg cgtgatgctg cggaagccgt cgacagggcg ctgcgacccg
     2941 accaggccga gatccgaaga aaggatcgtg ttgtcgacgc cggcgacctc gatcagatgc
     3001 gccagatcgt ccgcactgta tttttccgat ttgcccttga tgaacatgca gatcgagtgt
     3061 tccatgacca cacccagccc gaccaactgg cggatatcgc tgtcgccgaa tccgacgatg
     3121 taggttggat gattgaccat catcttcttg accccacgcc gcgccgcttc ggcgaacacc
     3181 aggtgctgct cggtcacatg aaggtgaccg cccgccaaga tgatgtcggc ctccgcaacg
     3241 agatcgatca cctttttggt ttcatcggtg agcctgccgt tggcgtccag cgcggaaagg
     3301 ggcgtcggat cgagcatctt ctgcgaggtc ttcggaaagg tcttggcatt gccgctggcc
     3361 agaacgttga tgtggtttgc cgccgacagc gtcggcatcc agacgctctt gccgccgagc
     3421 ttgatcgcgt ggtccacggc atgcgggttg atgccacccg acgcattgtt gagcgcgatg
     3481 cctgaataca atcgaacccc aagctccgga aatagttttt cgagcatgat cgcatgggcc
     3541 atgccgggat agaaatgatc cttgtagagc accgcccgaa agccggctgc cgccgcatcc
     3601 agcaattcgt catgatgatc caggatgcgc ggcatcgctg cggggccgct gtggcaatgc
     3661 agatcgacgg cgccgatcag aagttcctcg acttcggccg ggcgcgacag cggcggaagc
     3721 ggaaccgtgt cgggatagga aacctgatta ttggccatga cggtcgcgct caattcgtgt
     3781 tggtgttggt ctcggcctga ggcggcatcg gatactcgtc accgttcagg acccagcccg
     3841 gcttggtgga aaagtccatt cgcggcgggc agaagacatc gacgaggaga ttgagaccct
     3901 cgtatacggc ctgagaggta tgaatcgcgg gtggtgggat cacggctatc gacggggagt
     3961 cgcagcgctc atgggtgtcc tcacgccact ggcccatatc cggcgtccag ggccagcgca
     4021 tgtgatggat gaaggtgcct gcgagcgcca gcgagcactg ctcaaaatca tcatggtggt
     4081 gcggcgacat cttgctggtg tcgcgcgggc catttttcgg atcaagaaaa ttgaccatga
     4141 aggtcgagcc acgccagatg cgaccgaacc ggccgggcga cggcgcgacc tcgaggtcgt
     4201 aggtcctgat ctttggaccc gcggtcggct ctggccacgg ttcgaacgcg ggaaggttcg
     4261 gatgcgcgga agcgtaggat tccgcattcg agcagagagc caccagatct ttcgatttgg
     4321 tcgtcatcat ccgaatgatt ttggccggcc cgagcagcgt ggccgaactt ttgccggccg
     4381 gaacgaacgt aatcgaatgc gcggtgaccg tgatccgttc gttgtcccac acaatttcag
     4441 cgccggcgcc cggatccgga agcaaaacga catattcgtc gggctgttcg gcccggctca
     4501 gcaccgcgcc cttggtcgcc tcgctgtagg cgataatgaa gttctgaccg cgcgcatacc
     4561 agtttctcgc gccatccttc tcttcggccg gcggcatctc gtagaacttc gcatattccg
     4621 cgccggcaaa acgggtcggg ttcgactgaa tgccgaccgg cttcgccgca agcgcggcac
     4681 ggggatcgct gctatgatac atgggtgtgt tccggcgttt gaacgagctt gttgaaatcg
     4741 gtaaggcttt gcgcggctgc acgccgcatc tgaccctgat cggatgaaat gatgaatgcg
     4801 ctggcgccga gatcggcaaa atccttggct tcagcggcgc ttgcaaccat cacgcagacc
     4861 ggctttttcg cctgctgtgc cgccgcgatg acgcgtttga ctgcatcctg aactttcggg
     4921 gcagacgatt tcgcgccaag tgaaaccgtc agatcgccac gaccaatgaa caggccgtgg
     4981 ataccatcga cggcggcgat ctcgccgata tgatcgagcg cctcggggtc ttcgatcatg
     5041 gcgatcacgc ataccgacgc gtcctgttct tcgaccagtt gccacattgg cgtggcgcca
     5101 tagccacctg cgcgtggcga tcccgaatac ccgcggcgcc cgccgtggta ccgggagacc
     5161 tcagcgacgg cccgcgcctt ctcgaccgag gcgacatgcg gcaccagcac gccggtcgcg
     5221 ccgcaatcga gacaggacag gatcttggtc ggatcgcctg acgatacccg cacgatgctg
     5281 gcgagatttg acgcgcgcgc ggcaagcacg acgacgtcgg tgacggcgcg atcgaacggc
     5341 gcatgttcct catcgatcac gacaaaatcg taacccagcg cgccgaagat ctcgctggcg
     5401 tgggtggtcg gtgttttgat gaaactgccg accagattct gtccgcctgt tagcctgcgg
     5461 cggaacgacg aaaaatgcgg tttaacctca cctgacaacg cggtccctcc cgattattgt
     5521 tttgccttgc ctcagcccgt cgcgaatgac ggcagcgcag cacttgcgag agacattgca
     5581 gccgggccgg ctttcggcca tagctattcc ccgccgttcc gcacagtgga atttcaagcg
     5641 atgccgttac gttcggtcaa ggtcgaggcc gccgcccgca cgctggttct gctcgaagag
     5701 ctgaaccggc atcgcgtcgc ctcgatacag gttctgcatc gggcgacggg tcttccgaaa
     5761 tcgaccgtgg tgcggctgat gaaatcgctc tgcgccatgg gctacgccgc aaatgaccgg
     5821 cgacaaggcg gctataccgt ggcctcacgg gtcaaatcac tgagtaacgg cttccatggc
     5881 gatccactgg tcgtggaagc ggcgcgccca tgggcgcttg ccttcaccgc gcaatatcat
     5941 tggcctgtcg ccatcgcggt cctcgacggc gcctcagtcg tggtccggtt cagcaccatt
     6001 ccggacagcc cagtttcacc gtttcacggc accatcaaca tgcgcctcag cctgcttcgt
     6061 cgcgcgctcg gccgagccta tctggcgttc tgtcctgccg acgaacgcgg gatgctgctc
     6121 gacatgctgt cacgatcgac tgaccccgaa gaccggatcg cggtcgatcg caggcgaacg
     6181 caggcgttgc tcgctgtcat acgcaagcag ggctatgccg agcgcgaccc catggtcgag
     6241 ccgcgctcgt ccgggacgat cgccgtgccg atcatcggca aaggaaaggt gctcgcgacc
     6301 gtcggcatga cctatttcat gtcggcgctt gaccgacgcg acctcgcaca gcgttatgcg
     6361 ccgctcatac agactttggc ggacaacatc tcctccagcg tcgcgtcgct tcggtagatc
     6421 acgcaatgga cagcacgaag agtggcaagc gccgcgactg cagcgatctg cacttcgcgg
     6481 gcctgttttc gcaagcgtcc ctgctccagc ttttgaagcg gccaaggaaa ttgcagtgcc
     6541 tacggccgac gtcagtccgc aggaccaggc cctcatcgga gcaccgcttc caccgttctg
     6601 gaatgatcat cccaaggacg ccgccgcatg gaaggcgctg atccagacgc gcgctgatca
     6661 gatcatcaag acgctgccgg gcctgcgcga gaagttcggc gtgaagtccg agcagatgac
     6721 gatcgcgggc gtgaattgct atatgctcac tcccgacaac attcccgaac agaatcgcga
     6781 tcgcctgctg gttcacgtcc acggcggcgg ctacgtcttc gctccggccg aggccgcaac
     6841 gcgcgaagca atcctgatgg caggtttcgg caagttcaag gtgatctcga tcgactaccg
     6901 catgccaccg gattatccgt atccggctgc gatggacgac gcgatggcgg tgtggaagga
     6961 ggtcgtaaag acgaacgccc cgaacaagat ggcgatattc ggaacgtcca ccggcggcgg
     7021 gatgacgttc gcgatggtgc tgcgcgccag gacggaaggt ctgccgctcc ccgccgccac
     7081 ggcgcccggc accccatggt cggacatgac caagaccggc gacaccttct tcaccaacga
     7141 gaaggtcgac aacattctgg tgagcaacga cggctggctc ggcgatgccg cgaagctcta
     7201 tgccaacggc catgacctga aggatccgca actttcgccg gtctatggcg acctgtccgg
     7261 tttcccgccg accattctca catcgggcac gcgtgacctg ttcctgtcca atacggtacg
     7321 cgtgcatcgc aagctgcgtc aggccggcgt tgccgctgat ctccacgtgt tcgaaggcca
     7381 gtcacatgcg caatacgcgg gcgatcccga tgcacccgag acaaaggagc acttcggcga
     7441 gctaacggcg ttcttcgaca aatatctggc gaagtagggc cacgggcagg gtcgaatcga
     7501 tcgggatcga cgtctgaaga cgtcgggacg ggacggcttc gccgatcgct tccaaggggc
     7561 cacgacgttg ttctcgagag cgcaacgtct gcttctcgcc ctcagccgac tctaccgcac
     7621 cgttttctcg caggtccgct ttgcgccagc cgacactcgc tgacggtggt catacacttc
     7681 aactgtctgc gtccgcgaag ctgcgtcaag ggcgtgtgtc gccctgcccc acaaggggag
     7741 agagagagag agagcagacc gcgtacatag cacggctttg gattcaattt tcaaacagca
     7801 gctgtcaact cactccgtca ttgccgggct cgaccccggg gcatgacgag tgtcgacatc
     7861 tcaactccag acacacattc acgtcctcgc gacgcgaagc gcccgaggcg ttgatgaatc
     7921 gtccgcccaa agagggcgtg gggaacgccg ggtgcccact gcacccgcag cctcgcgtgc
     7981 actttgtagt ggtagaacgc acacgagtaa caacgagtac accggaatca cccggcattc
     8041 ccgcacgcaa tggttttaac ggtt
//
LOCUS       DATNOI010000176         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1996433,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000176 DATNOI010000000
VERSION     DATNOI010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996433"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..843)
                     /locus_tag="VN968_06865"
     CDS             complement(22..843)
                     /locus_tag="VN968_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HXL28994.1"
                     /translation="MWTAKTRGRIAAIEKKTKRYPTDLTDAEWERIRPLLPRPAKRGR
                     KPSVDLREILNAIRYMTRTGGGWRMLPKDFPPWQTVYWWFRRFVRLLLFRTIHDIALM
                     VDRERAGREASPTAGIADSQTVKAPAAKTRGYDAGKKIVGRKRHIVVDTDGRLLMVNL
                     TTADISDSAGAQAILAAIRQRWPWIKHLFADGAYDRTTLMDKAAFLDFVIEIVRRIDT
                     EPRFKVLPRRWVVERTFGWMTRWRRLVRDYEQRIDVSEAMIHVAMGSLLLRRISH"
     gene            complement(1129..>2195)
                     /locus_tag="VN968_06870"
     CDS             complement(1129..>2195)
                     /locus_tag="VN968_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXL28995.1"
                     /translation="VGWGECEAAPLPSIAAFVCPMSHGACRPVSASVLGETLDGPGDI
                     KRIAASLALNSMDLLQAPHTWSGIEVALWDLLGRVRGEPAWKLLGYSKAHPKTPYASV
                     LFGATPEQTLERARQLRAKSFKAVKFGWAPFGDSLAGDIAQLEAARQGIGPDGILLID
                     AGQVFGDNIDAAAERLAALNANRVTWFEEPFGGFAYDAYRRLADRSGTVRLAGGEAAH
                     NRYMAEHLIEYGHVGYIQIDCGRIGGLWPAKQVADFAAVKGVTYVNHTFTSNLALSAS
                     LQPYAGMQECRFCEYPTALSQLAVDLTSTRMVPNANGEIEVPDAPGLGVEINERALKA
                     YAVDVEIRVAGKTIFGTPVV"
BASE COUNT          389 a          697 c          684 g          425 t
ORIGIN      
        1 agagtctgtt tgagaatggg attaatggct gattcggcgc aagaggaggc tacccatggc
       61 gacgtggatc atggcctcgg agacgtcgat ccgttgctcg tagtcgcgca cgaggcggcg
      121 ccaccgggtc atccaaccaa aggtgcgctc gaccacccag cgccgtggca gcaccttgaa
      181 gcgtggttcg gtgtcgatgc ggcggacgat ctcgatgacg aagtcgagga aggcggcctt
      241 gtccatgagt gttgtccggt cataggcgcc atcggcgaac agatgcttga tccatggcca
      301 gcgctggcgg attgccgcga ggatcgcctg ggcgccagcg ctgtcggaga tatcggcggt
      361 ggtgaggttg accatcagga ggcggccgtc ggtatcgacg acgatatgcc gcttgcggcc
      421 gacgatcttc ttaccggcgt cgtagccgcg cgttttagcc gccggcgcct tcaccgtctg
      481 gctgtcggcg atgccggcgg tcgggctggc ctcgcggccg gcgcgctcgc ggtccaccat
      541 cagcgcgatg tcatggatgg tgcggaacag cagcagccgg acgaagcgcc ggaaccacca
      601 gtagaccgtc tgccagggcg gaaaatcctt gggcagcatg cgccagccgc cgcccgtacg
      661 cgtcatgtag cgaatggcgt tcagaatctc gcgcaaatca acactgggct tgcgtccgcg
      721 cttcgccggt cgcggcagca agggtctgat gcgctcccat tccgcgtccg tcaggtccgt
      781 cgggtagcgc ttggtcttct tctcgatggc ggcgatacgg ccgcgtgtct tggcggtcca
      841 catccccagc ttgaatcaca ccgctccgcg tcgctccaag cattttcaaa cagtctctta
      901 ggtcttcaat tgccgttcgc cgatccggaa cgccgggcgc catcattttc actgatctcg
      961 atggcatatg tgcagccaga cggtgcggcg gctctggccc acaatcgcat tgcggtgact
     1021 ttacaagcga aaacgcctct ctgagctggg cgcacgccta catggacagc acggtggaag
     1081 atcccgccgg tgcctccaga tccccgctcc caaatttcag cgcctcggct atacaaccgg
     1141 cgtaccaaat attgttttgc cggcgactct gatttccacg tcgacggcat aggccttgag
     1201 cgccctttcg ttgatttcga ccccgagccc gggagcatcg ggaacctcga tttcgccatt
     1261 ggcattgggg accatgcgcg tggaggtcag gtcgacggca agctggctga gcgccgtggg
     1321 atattcgcag aaccgacact cctgcatgcc ggcatagggc tgaagcgatg cactgagggc
     1381 gaggttcgac gtaaacgtgt gattcacgta ggtcaccccc tttaccgctg caaagtccgc
     1441 aacctgtttg gccggccaca aaccgccgat ccgcccgcag tcgatttgga tatagcccac
     1501 atgcccgtac tcgatcaggt gttcggccat gtagcggttg tgagccgctt cacccccggc
     1561 aagcctcacc gtgccgcttc gatcggcaag ccgtcgatag gcatcgtagg cgaagccccc
     1621 gaaaggttcc tcgaaccaag tgactctatt tgcattcagg gcggcaaggc gctccgctgc
     1681 ggcgtcgatg ttgtcgccaa agacctggcc ggcgtcgatc agcagtattc cgtcaggacc
     1741 tataccctgc cgggccgctt cgagctgggc gatatcgcct gcgaggctgt ccccgaacgg
     1801 cgcccatccg aacttcacgg ccttgaaact tttcgcgcgc aattgcctgg cccgctccag
     1861 ggtctgttcc ggtgtggcgc cgaacagcac cgatgcgtat ggcgttttgg ggtgcgcctt
     1921 tgaatagccc agcagcttcc acgccggttc gccccggacg cggccgagca gatcccacaa
     1981 tgccacctcg atgccagacc aagtatgggg cgcctgcagc aggtccatgc tgtttaatgc
     2041 aaggctcgcg gcgatccgct tgatatcccc ggggccgtcc agcgtctctc caaggaccga
     2101 cgccgaaact gggcggcagg cgccgtggga catggggcag acaaaggctg cgatcgaggg
     2161 cagcggtgcg gcctcgcatt ccccccaccc gacgt
//
LOCUS       DATNOI010000177         6228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_50968,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000177 DATNOI010000000
VERSION     DATNOI010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50968"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="VN968_06875"
     CDS             <1..99
                     /locus_tag="VN968_06875"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HXL28996.1"
                     /translation="SIPLRFSVSDPEGHLHVPLLVTPWSYATYRGS"
     gene            249..1088
                     /gene="pucL"
                     /locus_tag="VN968_06880"
     CDS             249..1088
                     /gene="pucL"
                     /locus_tag="VN968_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HXL28997.1"
                     /translation="MPLIKNKYGKGRVRVMRIHRDGDTHEVSQLNVKAMIEGDFARAY
                     THADNSTAVSTDTIKNVVNIVARQNTGLCTEEFCQVLAKKYLDTYPQVASVSVTAHET
                     KWSRLSFGGKPHPHSFVLDSNGKPSVEVTATREGSTLTSGIDGFTFMKSTQSGWENYV
                     KDPYTTIPETHDRMCATSMVASWKWSSKPANYPATNAKILATLLEVFGTTYSASVQDS
                     LYRMGEAALAAVPEISEISMACPNMHFILMNLSAFGLDNNNDVFLPTDEPHGQIECTV
                     GRG"
     gene            complement(1206..3929)
                     /locus_tag="VN968_06885"
     CDS             complement(1206..3929)
                     /locus_tag="VN968_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL28998.1"
                     /translation="MSYQINGKAFSERPQAGQCLRTFLRELGHFGVKKGCDAGDCGAC
                     TVLIDGEPVHSCIVPAFRAEGHAVTTIEGLAPDSGTHPMQQAFLDAQAFQCGFCTSGM
                     ILTCASLNQAQRQELGASLKGNLCRCTGYRAIEDALNGKTNIEGAAAGTAFGRSLPAP
                     AGPQLVRGAARYTFDTVIEGLLHIKMLRSPHPHAKIISIDKTAALAVAGVHAVLTFED
                     APDRLFSTARHEKAWMDPDDTRVLDNIVRFVGQKVAAVVAETEAAAEEGCRRLKVDYD
                     VLPDVIDPAQAIAAGAPRLHPDKTREHRVANAQRNIVAETHGEYGDVASALAQAAVSY
                     EGTFTTQRVQHAALETHGGLAWIDPTGILNVRSSTQVPFLTRRALTEIFDLPPDKVRV
                     FCERVGGGFGGKQEMFVEDVLALAAIKTGRPVKLELTREEQFISTSTRHPMRVTVKAG
                     ADSHGKLTALQLDVLSNTGAYGNHAGPVLFHSCGESLGVYNCANKKVDAVVAYTNTVP
                     SGAFRGYGLPQTLLAVEAAIDELARGLGISPYEIRRRNIVKPGDPMLSPPGPEHTDVF
                     YGSYGLDQCLDLVERAMQADGPNPELSADWLTGDGIALTMIDTVPPGGHLADARISLQ
                     DDGGFELTVGTAEFGNGTATVHRQIAATALATTVDQIRLLQSDTAHGGHDTGAYGSAG
                     TFVAGRATQAAAENLADDLRVFASTATKTNPDACVLENDSAVCGRQRLSFSALAEAAR
                     AQGHALSATGTSAGTPRSVAFNVQGFRVAVNKFTGEIRILKSVHAADAGRVANPMQCR
                     GQVEGGVAQSLGATLYEEMVIDDRGRVVNPKFRDYHLPSFADIPRTEVFFAETTDSLG
                     PMGAKSMSESPYNPVAAALGNALADATGIRFTAVPFKPDRLWPMLHEKFGK"
     gene            complement(3926..4753)
                     /locus_tag="VN968_06890"
     CDS             complement(3926..4753)
                     /locus_tag="VN968_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HXL28999.1"
                     /translation="MDLNTIIEVAHPQTRGQLPVWTAGDAWLAGGTWLFSEPQVHLTR
                     LIDLTDFKWPALAIGDTGLTVAATCTVAQLDALACPPEWLAAPLIDQCCRAFLASFKI
                     WKTATVGGNLCMSLPAGPMIALTAALDGVCTIWKADGSEQKIGVADFVIGNQSNVLAP
                     GDLLRQVDIPLTALKRRSAFRQISLTPVGRSAALLIGSVTSAGAMALTITASTTRPMQ
                     LSFPEIPAATELREAILHRIPDALYHNDVHGKPAWRKHMTLRLAQEIRAELLGGALP"
     gene            4910..>6228
                     /locus_tag="VN968_06895"
     CDS             4910..>6228
                     /locus_tag="VN968_06895"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HXL29000.1"
                     /translation="MDQPRAIWIKDPLGILADGAERGIVVRNGRIVELVPKGGQPTTP
                     AAFFEAGDHVVLPGLINTHHHFYQTLTRALPAALDRELFPWLQALYPVWARLTPEALN
                     LGVSVAMSELLLSGCTTTTDHHYVFPAGLEDAVDIEVAVARRLGVRVLLTRGSMNRSQ
                     RDGGLPPDSVVQDEDTILADSERVVAKHHQRGEDAMVQIALAPCSPFSVTTSLMRATA
                     ALAERLDVRLHTHLAETEDENKFCEQMHGCRPLDYLEQCGWLNARTWLAHGIHFTSEE
                     MKRLGKAKTTISHCACSNQLLASGCCPVCDMEQAGVQIGLGVDGSASNDGSNLMQEVR
                     AAFLLQRARYGVGRVSHKDALRWATRGSAACVGRPELGEIAVGKAADLALFKLDELRF
                     SGHGDPLAALVLCGAHRADRVMVGGNWVVTGGAIPGLDMPDLIRRHSA"
BASE COUNT         1104 a         1938 c         2040 g         1146 t
ORIGIN      
        1 tccattccat tgcggttttc ggtgagcgac cccgaaggtc acctccacgt accgctgttg
       61 gtcacgccct ggagctatgc gacctatcgg gggagttagt cctcgtccca aggccgcatc
      121 ccaaaaccgc gccgaagcgg ccatgcttcc gaacagcgct tgcccggtgg cacgaacgtt
      181 gctcattatt gggccggacc ggggtgaaac gagacccgcg cggaattgcc ggaactggag
      241 gctctcacat gccgctcatc aagaacaaat atggcaaagg ccgggtccgc gtcatgcgga
      301 tccatcgtga tggcgacaca cacgaggtca gccagctcaa cgtcaaggcg atgatcgagg
      361 gcgactttgc gcgcgcttat acccacgccg acaattccac ggcggtctcg accgacacca
      421 tcaagaatgt ggtcaatatc gtcgcgcgcc agaacaccgg cctctgcacc gaggaattct
      481 gccaggtgct ggcgaagaaa tatctcgata cctatccgca ggtggcctcg gttagcgtga
      541 cggcgcatga gacaaaatgg agccgcctga gtttcggcgg caagccgcac ccccacagtt
      601 tcgtgctcga cagcaacggc aagccgtcag tcgaggtgac cgcaacccgt gagggatcga
      661 cgctcacctc gggtatcgac ggcttcacgt tcatgaagtc gacccagtca ggctgggaga
      721 actacgtcaa ggatccctat acgacgatcc ccgagaccca cgaccggatg tgcgcgacca
      781 gcatggtggc gtcgtggaaa tggtcgtcga agccggcgaa ctatccggcc accaacgcga
      841 aaattctcgc gaccttgctg gaggtgtttg gcaccaccta cagtgcaagc gtgcaggaca
      901 gtctgtaccg gatgggtgag gcggcgctgg ctgccgtgcc ggaaatttcc gagatcagca
      961 tggcgtgccc gaacatgcat ttcatcctga tgaacctgtc ggcgttcggg ctggataaca
     1021 acaacgacgt gttcctgccg accgacgagc cgcacggcca gatcgaatgc acggtggggc
     1081 gggggtgatg cgagcagacg ccgcgtcgag ttcgtcgtca ttgccgggct tgacccggca
     1141 atccatctgc cagggaaaac accctttttg atggatgcgc gggtcaagcc cgtgcatgac
     1201 gagtgctatt tcccaaactt ctcatgcagc atcggccata gccggtcggg cttgaacggc
     1261 actgccgtga agcggatgcc ggtggcatcg gcaagggcat tgccgagggc tgcggcgacc
     1321 ggattgtagg gactttcgct catcgatttc gcgcccatcg gccccaggct atcggtggtt
     1381 tccgcgaaaa acacttcggt gcgcgggata tcggcgaatg acggcaaatg atagtcgcgg
     1441 aatttcggat tgaccacgcg gccccgatca tcgatcacca tctcctcata gagcgtagcg
     1501 ccgagcgact gcgcgacccc gccctcgacc tgtccgcggc actgcatcgg attggcgacc
     1561 cggccggcat ccgcggcgtg cacgcttttg aggatcctga tttcgccggt gaacttgttc
     1621 acggcgacgc gaaagccctg gacgttgaac gcgaccgacc gcggcgtgcc tgccgaggtg
     1681 ccggtcgcgc tgagcgcgtg tccctgcgcg cgggcggctt cggcgagcgc ggaaaacgac
     1741 agtcgttgcc tgccgcagac cgcggaatcg ttttccagca cgcaagcatc gggattggtt
     1801 ttcgtcgcgg tggaagcaaa aacccttaga tcgtcggcca gattttccgc cgcggcctgc
     1861 gtcgcgcggc cggcgacgaa ggtgccggcg ctgccatagg cgccggtgtc gtggccgcca
     1921 tgcgcggtgt cggactgcag caggcggatt tgatcgaccg tggtcgccag cgcggtcgcg
     1981 gcgatctggc gatgcaccgt ggcggtgccg ttgccgaact ccgccgttcc cacggtcagt
     2041 tcgaagccac cgtcgtcctg cagcgaaatt ctggcgtcgg ccaaatgccc gcctggcggc
     2101 acggtgtcga tcatggtcag ggcgatgccg tcgccggtca gccagtcggc ggagagctct
     2161 gggttcgggc catcggcctg catcgcgcgc tcgaccagat cgaggcactg atcgagccca
     2221 tagctgccat agaacacgtc ggtatgttcg gggccgggag gcgacagcat cggatcgccc
     2281 ggtttcacga tgttgcggcg gcggatttcg taagggctga tgccgagccc cctggccagt
     2341 tcgtcgatcg cggcttccac cgccaacagc gtctgcggca gaccgtaacc ccggaacgcg
     2401 cccgatggca cggtgttggt ataggcgacg acggcatcga ccttcttgtt ggcgcagtta
     2461 tagacgccaa gcgactcgcc gcacgaatga aacagcaccg gcccggcatg attgccatag
     2521 gccccggtgt tcgagagcac gtcgagctgc aatgccgtca gttttccatg gctgtcggcg
     2581 ccggccttga cggtgacgcg catcggatgc cgcgtcgagg tcgagatgaa ttgttcttcg
     2641 cgggtgagtt caagttttac cggccgtcct gtcttgatgg cggccagggc aagaacatct
     2701 tcaacgaaca tttcctgctt gccgccaaaa ccgccgccga cgcgttcgca gaacacccgg
     2761 accttgtccg gcggaaggtc aaaaatttcc gtcagcgcgc gccgcgtcag gaacggtact
     2821 tgcgtgctgg agcgcacgtt caggatgccg gtcggatcga tccaggcgag cccgccatgg
     2881 gtttccaggg cggcgtgctg gacgcgctgg gtggtgaagg ttccctcata gctgaccgct
     2941 gcttgggcca gggcgctggc gacatcgcca tactcgccat gggtttccgc cacgatgttg
     3001 cgttgcgcat tggcgacgcg atgctccctg gtcttgtcgg ggtggagccg gggcgcgccc
     3061 gctgcgatcg cctgcgccgg atcgatcacg tcaggcagaa cgtcgtagtc gaccttgagg
     3121 cgccggcagc cttcctccgc ggcggcctcg gtttccgcga ccacggcggc gaccttttgg
     3181 ccgacgaagc ggacgatgtt gtccagcacc ctagtgtcgt ccggatccat ccaggccttc
     3241 tcgtgccgcg cggtcgagaa cagcctgtcc ggcgcgtctt cgaaggtcag caccgcatgc
     3301 acgccggcaa cggccagtgc cgcagtcttg tcgatcgata tgatttttgc gtgcggatgc
     3361 ggcgagcgca gcatcttgat atgcagcaat ccctcgatca ccgtgtcgaa ggtgtagcgc
     3421 gccgcgccgc gcaccagctg cggtccggcg ggtgcgggca ggctgcggcc gaaggcggtg
     3481 ccggcggcag cgccttcgat attggtcttg ccgttgagcg cgtcctcgat cgcgcgatag
     3541 ccagtgcagc ggcatagatt gcctttcagc gaggcgccaa gctcctgccg ctgcgcctgg
     3601 ttcagggatg cgcaggtcag gatcatgccg gaggtgcaga agccgcactg gaatgcctgc
     3661 gcatcgagaa acgcctgctg catcgggtgg gtgccgctgt ccggcgcgag gccctcgatg
     3721 gtggtgacgg catggccctc ggcgcggaac gccgggacga tgcagctatg caccggctcg
     3781 ccatcgatca gcaccgtgca ggcgccgcaa tcgccggcgt cgcagccttt cttgacgccg
     3841 aaatgcccga gttcgcgcaa aaaagttcgc aggcattgtc cggcctgcgg acgttccgaa
     3901 aacgccttgc cgttgatctg atagctcatg gcaatgcgcc gcccagcaat tcggcgcgaa
     3961 tttcctgcgc caggcgcagc gtcatgtgct tgcgccaggc cggcttgccg tggacgtcgt
     4021 tgtggtagag cgcatcgggg atgcgatgca ggatcgcctc gcgcagttca gtcgcggctg
     4081 gaatttcggg aaaagagagt tgcatcggcc gcgtcgtgga tgccgttatc gtcaaggcca
     4141 tggcgcccgc actggtgaca ctgccgatca gcagcgccgc cgaccggccg accggggtca
     4201 acgatatctg gcggaatgcc gagcgccgtt tcagcgctgt cagagggata tcgacctgcc
     4261 gcaacagatc gcccggagcc agcacgttgc tttggttgcc aatgacaaaa tcggcgacgc
     4321 cgattttctg ctcgctgccg tcagccttcc agatcgtaca gacgccatcg agtgctgccg
     4381 tcagtgcgat catcggtccg gccggcaaag acatgcagag attaccgccg acggtcgcgg
     4441 ttttccagat cttgaaggac gccagaaagg cccggcagca ttggtcgatc aggggggcgg
     4501 cgagccattc gggcgggcag gccagcgcgt caagctgcgc gaccgtgcag gtcgcggcga
     4561 ccgtcaaccc ggtgtcgccg atcgcgagcg ccggccattt gaaatcggtg agatcgatca
     4621 gccgcgtcag atggacctgg ggttccgaga acagccaggt gccgcccgcg agccaagcat
     4681 cgcctgccgt ccaaacgggc agttgtcccc gcgtttgcgg gtgcgcgact tcgatgatgg
     4741 tgttcagatc catgaatata ccagagctat caacgagata cttctccctg ctgaagtctt
     4801 gcaaaaccga cgccaactgt cgtctaacga ttgcgttggt ctttgaattg caagggcaag
     4861 tgtggcaacg cttttcatgc atcttaatcc ttcggagaag cttcacgcca tggatcaacc
     4921 gcgggcgatc tggatcaagg atccgttggg cattctcgcc gatggtgcgg agcgcggcat
     4981 cgtcgtccgg aacggcagga tcgtcgagct ggtgcccaaa ggcggccagc cgacgacacc
     5041 cgcggcgttc tttgaggcgg gcgaccatgt cgtgctgccg ggactgatca acacccatca
     5101 tcacttctac cagacgctga cgcgcgcgct gccggcggcg ctggaccgcg aattgtttcc
     5161 gtggctgcag gcgctttatc cggtgtgggc gcggctcacg cccgaagccc tcaaccttgg
     5221 cgtctccgtg gcgatgtcgg agctgctgtt gtcgggctgc accaccacga ccgaccatca
     5281 ttacgttttt cctgccgggc tcgaggatgc cgtcgatatc gaggtcgcgg tcgccaggcg
     5341 gctcggcgtt cgcgtgttgc tgacgcgcgg ctcgatgaac cggtcgcagc gcgacggcgg
     5401 actgccgccg gatagcgtgg tgcaggatga agacaccatc ctcgccgaca gcgaacgcgt
     5461 ggtggccaag caccaccagc gcggcgagga cgccatggtg caaatcgcgc tggcgccgtg
     5521 ttcgccgttc tcggtgacga cgtcgttgat gcgtgcgacg gcagcccttg ccgagcggct
     5581 cgacgtccgg ctgcatactc acctggcgga gaccgaggac gaaaacaagt tctgcgagca
     5641 gatgcatggc tgccggccgc tcgactatct cgagcagtgc gggtggctca atgcccgcac
     5701 ctggctggcc cacggcattc attttacctc cgaggagatg aagcgcctcg gcaaggcaaa
     5761 gacgaccatc agccattgcg cctgcagcaa ccagctgctg gcatccggct gttgtccggt
     5821 ctgcgacatg gaacaggcgg gggttcaaat cgggctcggc gtcgatggct cggcctcgaa
     5881 cgatggatcc aatctgatgc aggaggtgcg tgcggcattc ctgctgcagc gggcgcgcta
     5941 tggtgtcggc cgggtcagcc acaaggacgc gctgcgctgg gctaccaggg gatcggcggc
     6001 ctgcgtcggc cgtcccgaac tgggcgagat cgccgtcggg aaagccgccg atctggcgct
     6061 gttcaagctc gacgaattgc gtttctccgg ccacggcgac ccattggcgg cgctggtcct
     6121 gtgcggcgcg catcgcgccg acagggtgat ggtcggagga aactgggtgg tgacaggtgg
     6181 cgccattccc ggcctcgaca tgcccgacct gatccgccgc cacagcgc
//
LOCUS       DATNOI010000178         2162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_255582,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000178 DATNOI010000000
VERSION     DATNOI010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255582"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VN968_06900"
     CDS             <1..1182
                     /locus_tag="VN968_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HXL29001.1"
                     /translation="ALYADLGHFGKRPIQTAWLFIVLPSLALNYLGQGALVIADPKAV
                     ENPFFLMFPDWALIPMVVLATLATVIASQAVITGAYSLTRQATQLGLLPRFEIRHTSE
                     SHSGQIYIPRINMLLFISVILLVLMFRSSSALASAYGIAVTGTMVVTAMMGFVVIWRV
                     WRWSAFAAAALIAPFLFLDLTFLAANLLKVFEGGWVPLALGAVVMLLMYTWRRGSRLL
                     FEKSRKLEFPLADLVAMLEKRPPQRVSGTAVFLTSDPASAPTALMHSLKHYKVLHEKN
                     VILTIETAPTPRIDPAERVRLEQISATFSKVKLRFGFMESPNVPKALAIARKLGWQFD
                     IMSTSFFLSRRALKPAAHSGMPRWQDHLFIALSRTANDATDYFQIPSGRVVEVGTQVT
                     I"
     gene            1371..1697
                     /locus_tag="VN968_06905"
     CDS             1371..1697
                     /locus_tag="VN968_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXL29002.1"
                     /translation="MADQVHDLTPEDVAKGMAEGRYLLVDVREPNEVAVEAYPDAVVV
                     PLSTFDPQDIPDPDGKQVVFACRSGKRSVTASLAAQAAGLAHDKHLAGGLLAWKAAGL
                     PTKTGG"
     gene            1706..>2162
                     /locus_tag="VN968_06910"
     CDS             1706..>2162
                     /locus_tag="VN968_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXL29003.1"
                     /translation="MTSMNKVFADLPVTVFEAMSQLARDNNAINLGQGFPDDPGPEDI
                     RRAAAEAVMDGYNQYPSMMGIPELRQAIAAHYGRWHGLVLDPLTEVMVTSGGTEALTS
                     AILAVVEPGDEVVAFQPVYDSYLPIIRQAGGIPRLVRLEPPHWRLTEEAL"
BASE COUNT          382 a          638 c          686 g          456 t
ORIGIN      
        1 gcgctctatg ccgacctcgg gcattttggc aaacggccga tccagaccgc ctggcttttc
       61 atcgtattgc cgtcgctggc gctgaactat ctggggcagg gcgctctggt catcgccgat
      121 ccgaaagcgg tggaaaatcc cttcttccta atgtttccgg actgggcatt gatcccgatg
      181 gtcgttctcg ccaccctggc gacggtcatt gcaagccagg ccgtgatcac cggcgcctat
      241 tcgttgacgc gtcaggccac ccagctcggc ctgttgccgc gattcgaaat ccgccacacc
      301 tcggaatccc attccggcca gatctatatc ccgcggatca atatgctgct gttcatcagc
      361 gtgattctgc tggtgttgat gttccgctcc tcgagcgcgc tggcatcggc ctatggcatt
      421 gccgtgaccg gcaccatggt ggtcacggcg atgatgggtt ttgtcgtgat ctggagggtc
      481 tggagatggt cggccttcgc ggccgcggcg ctgattgcgc cattcctgtt tctcgatctg
      541 acgtttctcg cggcgaacct gttaaaagta ttcgagggcg gttgggtgcc gctggcgctc
      601 ggagccgtgg tgatgctgct gatgtacacc tggcggcgcg gcagtaggct tctgttcgag
      661 aaatcgcgca agctggaatt tccgctggcc gatctggtgg cgatgctgga aaagcggcca
      721 ccacagcggg tttccggtac tgccgtattc ctgaccagcg atcccgccag cgcgccgacg
      781 gcgctgatgc acagcctcaa gcactacaag gtgctgcacg agaagaacgt catcctgacc
      841 atcgaaaccg cgccgacgcc gcggatcgat ccggcggagc gggtgaggct ggaacagatc
      901 agcgcgacct tctccaaggt gaagctgcgg tttggattta tggaatcgcc caacgtgccg
      961 aaagcgctgg cgatcgcgcg caagctgggt tggcaattcg acatcatgtc gacgtcgttc
     1021 ttcctgtcgc ggcgcgcgct caagccggcc gcgcactccg gaatgccccg ctggcaggat
     1081 caccttttca tagcgctcag ccgcaccgcc aacgatgcaa ccgactattt tcagattccg
     1141 agcggacggg tggtcgaggt gggaacgcag gttaccatct aagtctcaag ggtgccttgc
     1201 ggagcttgat tttcgtcgcg tgaggggcga ggttggcgcc gaagggcgag caatccgtat
     1261 ccgtcctgtt ggattcgctc gacctcgcgc gtattccgca aaagtggata tcgattttgc
     1321 gaatagaata cgcgcaagtt gatgcactga tgatcggtgg gaggatttga gtggcggacc
     1381 aggttcacga tttgacgccg gaagacgtag cgaagggaat ggccgagggc cgctacctgc
     1441 ttgtcgatgt ccgcgagccg aacgaggttg cggtcgaagc ttatcccgac gcggtcgtgg
     1501 ttccgctttc gacttttgat ccgcaggata ttccggatcc cgatggcaag caggtggtct
     1561 ttgcctgccg ctcagggaaa aggtcggtga cggcatcgtt ggctgctcaa gccgcaggct
     1621 tggcccacga caagcacctg gcgggcggct tgctggcctg gaaggccgcg ggactaccga
     1681 ccaagaccgg tggctgacca ttgccatgac gtccatgaac aaggtgtttg cggatcttcc
     1741 cgtgaccgtc ttcgaggcga tgtcgcagct cgcgcgcgac aacaacgcca tcaatctcgg
     1801 ccagggtttt cccgacgatc ccggcccgga agacattcgc cgcgccgcgg cggaagcggt
     1861 gatggacggc tacaaccaat acccatcgat gatgggcatt ccggaattgc gacaggcgat
     1921 cgcagcccat tacgggcgct ggcatggcct cgtgctcgat cccctgaccg aggtgatggt
     1981 gacatcgggc ggcactgaag cgttgaccag cgcgatcctg gccgtggtcg agccgggcga
     2041 tgaggtcgtt gcttttcagc cggtctatga ttcttacctt ccgatcatcc gccaggccgg
     2101 aggcatcccg cgtctggtgc gcctcgaacc gccgcattgg cggctcaccg aggaggcgct
     2161 gc
//
LOCUS       DATNOI010000179         3429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1305505,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000179 DATNOI010000000
VERSION     DATNOI010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305505"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VN968_06915"
     CDS             complement(<1..423)
                     /locus_tag="VN968_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXL29004.1"
                     /translation="MKYFRVMGLALAAAFCAGQANAQELAGTLKNIKETGAITLGHRD
                     SSIPFSYLDDNQKPIGFAMDICYKIVDAVKKQLKLDKLEVKLNPVTSSTRIPLLANGT
                     IDLECGSTTNNAERQKQISFTNTHFLTASRFVSKKSSKI"
     gene            complement(538..1923)
                     /locus_tag="VN968_06920"
     CDS             complement(538..1923)
                     /locus_tag="VN968_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXL29005.1"
                     /translation="MTRTDAIARARQQLHSGEFLAELDRRVAYQTESQNPERRDCLRA
                     YLVDELTPAFAQLDFSTRLIESPSGKHPYLLAEYRESSPAPTVLMYGHGDVVEGMAGE
                     WRDNLDPWRTTTSGDRVYGRGTADNKGQHSINLSALRAVREARGGRLGFNAKFIIETG
                     EEIGSPDLRQVCESLRDELAADLFLASDGPRLAADRPTIFLGCRGGVRIHLDVNLRDG
                     SHHSGNWGGVLANPATILASAIASLVDGQGRLLLDQLKPPRISNQIRATLADVKIEPT
                     AGEPALSENWGEEGLSAAERLYAWNTLEVLAISSGNIEKPANAIPGRAQALLQLRFVV
                     GTRADKAADAVRAHLQRNGFPMVEVRAAQSFAASRTDFDSPWINWTAESIRQTTGKAP
                     AVLPNFGGSLPNDVFSDSLGLPTIWVPHSYPGCSQHAPDEHILLPVTEEALGIMAGLF
                     WDLGEMHRPRP"
     gene            2046..>3429
                     /locus_tag="VN968_06925"
     CDS             2046..>3429
                     /locus_tag="VN968_06925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXL29006.1"
                     /translation="MKKEIRLNAFAMNCVAHQSPGLWTHPRDRTSEYNRLPYWIHLAQ
                     TLERGRFDGLFLADVLGVYDVFGGSPDAALRNAAQTPANDPLLLIPAMATVTQNLGFG
                     VTSNLSYEPPFTFARRMSTLDHLTEGRIGWNVVTGYLDSAARAAGRDKQTAHDDRYGI
                     ADEYMEVVYKLWEGSWEDDAALRDRVSGVFADPAKVHRIRHEGNNYRVDAIHLSEPSP
                     QRTPVLYQAGTSPRGRQFAAEHAECVFMSGPSAKIIAPRVAAIRELAAKAGRNPAEIL
                     MFSMFTIILGRTEAEAAAKYADYIRHISPEGALTLMSGWTGVDFSTYDLDQQVRHVQN
                     DAGRTAMDNITRADPDRVWTVREVAEHVGIGGIGPVVVGTPEIVADAIEAWFEQTDVD
                     GLNVPFAISPGDFEDITDMLVPELTRRGRYKTAYQPGTLREKLFGKGRARLAAPHPAV
                     QYRPHVAAKAA"
BASE COUNT          647 a         1076 c         1073 g          633 t
ORIGIN      
        1 gatcttgctg gatttcttcg aaacgaagcg gctggcggtg agaaaatggg tgttggtgaa
       61 ggaaatctgc ttctggcgct cggcgttatt ggtggtcgaa ccgcattcga ggtcgatggt
      121 gccgttggcc agcaatggaa tgcgggtcga tgacgtcacc ggattgagct tgacctcgag
      181 cttgtcgagc ttgagctgct ttttcacggc atcgacgatc ttgtagcaga tgtccatggc
      241 gaagccgatc ggcttctggt tgtcgtcgag gtaagagaat ggaatcgacg agtcgcgatg
      301 gccgagcgtg atcgcgccgg tctccttgat gttcttcaat gtcccggcga gttcctgggc
      361 gttggcctgc cccgcgcaga acgcggccgc gagcgcaagg cccatcacac gaaaatattt
      421 catttttctt ctccccctgt tggacggctt tcgcaatgct tgcataaatc gggccggaat
      481 agaatgtaac tttcggccgc ccgacgttcc cgaacggcgg caatcaatag cctcgtctca
      541 aggccgcggc cggtgcatct cgcccaaatc ccagaacagg cccgccatga tgccaagcgc
      601 ctcttcggtg accggcagca gaatatgctc atcgggcgcg tgctgcgagc agccgggata
      661 ggaatgcggc acccagatcg tgggcaagcc gagtgaatcg gaaaacacgt cgttgggcag
      721 cgagccgccg aaattcggca gaaccgcggg cgcttttccc gtggtctgcc ggatcgactc
      781 cgccgtccag ttgatccagg ggctgtcgaa atcggttcgg gacgcggcaa agctttgcgc
      841 ggcgcgcacc tcgaccatcg gaaagccgtt ccgttgcaga tgcgcccgga ccgcatccgc
      901 agccttgtcg gccctggtgc cgacaacaaa gcgcaactgc agcaacgctt gcgcccgccc
      961 cggaatcgca ttggcgggct tttcgatatt gcctgatgat atcgccagca cttcgagcgt
     1021 gttccatgca taaagccgct cggccgccga caagccttcc tcgccccaat tctccgacag
     1081 tgccggctca ccggcggtcg gctctatctt gacgtcggcc agcgtagcgc ggatctggtt
     1141 cgagatgcgc ggcggcttta gttgatccag caacagacgc ccctggccat cgaccaggct
     1201 tgcgatcgcg ctggccagga tggttgcggg attggccagc acgccgcccc agttgccgga
     1261 atgatgcgag ccatcgcgca agttcacgtc gagatgtatc ctcacgcccc cgcggcagcc
     1321 gaggaatatg gtgggacgat cggccgcaag ccgcggcccg tcggatgcca gaaacagatc
     1381 ggcggccagc tcatcgcgca gcgattcaca gacctggcgc aagtccggcg acccgatctc
     1441 ctcgccggtc tcgatgatga atttggcatt gaagccgagc cggccaccgc gggcctcgcg
     1501 cacggcccga agcgccgaca gattgatgct gtgctgccct ttattgtcgg cggtgccgcg
     1561 accgtaaaca cgatcgcctg atgtcgtggt ccgccacgga tcaaggttgt cgcgccattc
     1621 tcccgccatg ccctcaacca catcgccgtg cccgtacatc agaaccgtcg gcgccggtga
     1681 actttcgcga tattccgcga gaagatatgg atgcttgccg ctcggggatt cgatcaatcg
     1741 tgtcgagaaa tcgagttgcg cgaatgccgg cgtgagctcg tccaccagat aggcgcgcaa
     1801 gcaatcgcgc cgctcggggt tctggctttc ggtctgatag gcgaccctgc ggtcaagttc
     1861 ggcgagaaac tcgccggaat gaagctgctg gcgcgcccgg gcgatagcgt ccgttctggt
     1921 catgtctttc tttccaaagc tcggatcagg aaacctatcg ggatatcgcg cccgccggaa
     1981 gtgggcgttg ccagtcggaa ttttggcttg ccaaaagctg ccggaatgca acaatttcca
     2041 ccatcatgaa aaaagaaatc cggctcaacg ccttcgccat gaactgcgtc gcgcatcaat
     2101 cgccggggct gtggacccat ccgcgcgacc gcacgagcga atataaccgg ctgccgtact
     2161 ggatccactt ggcgcaaacg ctggagcgcg ggcggttcga cggcctgttc ctggctgatg
     2221 tgctcggcgt ctacgacgtg ttcggcggca gtccggacgc cgcgctgcgc aacgcggcgc
     2281 aaacgccggc caacgatccg ctgctgctga tcccggcgat ggccaccgtg acgcagaatc
     2341 tcggcttcgg cgtgacatcc aacctgtcct acgagccgcc ctttacgttt gcgcggcgga
     2401 tgtcgacgct cgaccatctc accgaagggc ggatcggctg gaacgtggtg accggctatc
     2461 tcgacagcgc ggcgcgggcc gccggcaggg acaagcagac cgcgcatgac gaccgttacg
     2521 gcatcgccga cgaatatatg gaggtggtct acaagctctg ggaaggaagc tgggaggacg
     2581 acgccgcatt gcgcgatcgt gtcagcggtg tgttcgccga tcccgccaag gtgcatcgta
     2641 tcaggcacga ggggaacaac tatcgggtcg acgcgattca tctgagcgag ccgtcgccgc
     2701 aacgcacccc cgtgctgtat caggccggca cctcgccgcg cggacggcag ttcgcagcag
     2761 agcatgccga atgcgtgttc atgtcgggac cgtcggccaa gatcatcgcg ccgcgcgtcg
     2821 ctgccattcg cgaactcgcc gcgaaagccg gccgcaaccc ggccgaaatc ctgatgttca
     2881 gcatgttcac catcattctc gggcgaaccg aagcggaagc cgccgccaaa tatgccgact
     2941 atatcaggca tatcagcccc gaaggcgcgc tgaccttgat gtctggctgg accggcgtcg
     3001 atttctcgac ctacgatctc gaccagcagg ttcgccacgt gcagaacgat gccggccgca
     3061 ccgcgatgga caatatcacc cgcgccgacc cggaccgggt atggaccgtg cgcgaggtcg
     3121 ccgagcatgt cggcatcggc ggcatcgggc cggtggtggt cggcacgccc gaaatagtcg
     3181 ccgacgcgat cgaagcctgg ttcgagcaga ccgacgtcga tggcttgaac gttccgtttg
     3241 cgatctcgcc cggagatttc gaagatatca ccgacatgct ggtgccggag cttacccggc
     3301 gcgggcgcta caaaaccgcc taccagccgg gcacgttgcg ggaaaaactg ttcggcaagg
     3361 gccgcgcgcg cctcgccgcg ccgcatccgg ccgtgcagta tcggccgcac gtagcagcga
     3421 aggcggcgg
//
LOCUS       DATNOI010000180         5138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1117463,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000180 DATNOI010000000
VERSION     DATNOI010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117463"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VN968_06930"
     CDS             <1..1249
                     /locus_tag="VN968_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXL29007.1"
                     /translation="TSAAKLAVAREALTECPKVELCIVMDGPSEGDRIVNLLEATSGL
                     PRTPISDECVGTAMLYSSGTTGRPKGILRPLPEQPPTQQLPMFDFLQKLWQYREGMIY
                     LSPAPLYHAAPQAAVNLTIRNGGTVIIMESFDPERYLELVEKWGVTHSQLVPTMFSRM
                     LKLPEAARNRYDLSSLEIAIHAAAPCPAMVKDDMIKWWGPLIQEYYGATEGLGFTACS
                     SAEWLAHRGTVGKVLLGDLHILDENMQPCPVGTPGTVWFKTAARFEYFNDPNKTKEAR
                     SPDGSMSTVGDVGYVDADGFLYLTDRATFMIISGGVNIYPQECENLLITHPKIADAAV
                     FGVPNADLGEEVKAVVQPMPGILPGPDLVDELMLFCSQSLSRQKVPRSIDFEAELPRL
                     PTGKLYKRLLRDRYWGDKTSRIL"
     gene            complement(1264..2679)
                     /locus_tag="VN968_06935"
     CDS             complement(1264..2679)
                     /locus_tag="VN968_06935"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HXL29008.1"
                     /translation="MNRGMLHRAAIAVGCTAWVMGPALAEEPARLSGYNANISESSVS
                     GISSGAFMAVQFGTAWSSVIKGVGVVAGGPFWCAKADADDFINAFTLPIMTATGSCMS
                     GPLSDLSSFFAKADAKSASGDIDSLQFVRRQKVYIFHGYNDAVVARPVTDATAAFYRH
                     YLAETNHGNLYYQTAIGAGHSLVVAQDPHKDGLNDCKDNAVPFIDACGYDQAGIILQH
                     IYGALNAPNRGQLTGTAKRFDQSVYTKPDDPGTLSLGEAGYVFVPRPCEDGEACRVHI
                     ALHGCKQDTGDIGRRFVDDTGYNSWADTNHLIVLYPQTTSSSLLPFNPEACWDWWSYV
                     DHEDSYVTKSGSQIRTIKAMLDALTAHAVPAPAPAVAPTSLTVIDASDTGADLAWTPQ
                     AGTTVYRVRRAGADGQFAPLGDVAGASFADSGLTPQTAYRWRISAVANGIEDTPFIEA
                     AAVTRAAPPPCENPGSCAIGK"
     gene            2878..3765
                     /locus_tag="VN968_06940"
     CDS             2878..3765
                     /locus_tag="VN968_06940"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HXL29009.1"
                     /translation="MDVVPLGPGFAAELRGVTLKDVASDEVAYAATRTAFEEHSVLVL
                     RGQEMTDELQLAFSRRFGPPEVTKVGSQGTGSHFVILSTIGPDGKVVPSDHRQALRNR
                     ANQLWHTDSSFKRVPALTSILSARIIPARGGETEFVSTRLAFEHLDADLRNRLENSFA
                     WHDYAHSRGQISADLASPEERAALPPQCWRMVWKNPVHGRGAIYLASHAYAVEGMEAA
                     AGKKLIDELMAAATAPGTSYLHQWRGGDVVMWDNRATMHRGRPWPAHEARLMVRTTIS
                     ATEADGVATMHRPSPQAAE"
     gene            complement(3851..4096)
                     /locus_tag="VN968_06945"
     CDS             complement(3851..4096)
                     /locus_tag="VN968_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXL29010.1"
                     /translation="MMYPRRFARVRMTGKSSKAKLIVGPKAPIIDCNIIDYSAGGACL
                     EVDPNTRLPNRFELVFGVTKKRCRVVWTAGRRLGVVF"
     gene            complement(4358..5125)
                     /locus_tag="VN968_06950"
     CDS             complement(4358..5125)
                     /locus_tag="VN968_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL29011.1"
                     /translation="MLALNATIEAARAGEAGRGFAVVASEVKSLSHQTAQSTEEINRL
                     VAEIQSAADAAATSVGQIGQEIGEVDRVASSIAAAIQQQGAATREIARSVEQSAQSSR
                     EVSSRIANVSGGAEEVSIRAVEVQGAIANASANIAALRSILVRVVRTSSEDADRRVFP
                     RFRIDVPLTVTNSRESRTASKLIDISQGGARIASVPDLSLRETCSLHIGGVAAPIAFI
                     VRDKTEHQTLLELAEEGEPRQQYLAWLQRQISGLVAA"
BASE COUNT          995 a         1621 c         1564 g          958 t
ORIGIN      
        1 cacgtccgcg gcaaagctcg ccgtcgcgcg cgaggcgctc acagaatgcc ccaaggtcga
       61 gctctgcatc gtcatggatg gtccaagcga aggcgaccgc atcgtcaatc tgctggaggc
      121 gacgagcggg ctgcccagga cgccaatatc agacgaatgc gtcggcaccg cgatgctgta
      181 ttcgtccggc accaccggcc ggccgaaggg cattctgcgg ccgctgccgg agcagccgcc
      241 gacccagcag ctgccgatgt tcgacttcct gcaaaagctc tggcagtacc gcgagggcat
      301 gatctacctg tcgcccgcgc cgctgtatca tgcggcgccg caggccgccg tcaatctcac
      361 gatccgcaac ggcggcaccg tcatcatcat ggaaagtttc gatcccgaac gctacctcga
      421 gctggtcgag aagtggggcg tcacccacag ccagctggtg ccgaccatgt tctcgcgcat
      481 gctgaagctg ccggaggcgg cgcggaaccg ctatgacctg tcatccctcg agatcgcgat
      541 ccacgccgcg gcgccctgcc cggcgatggt gaaggacgac atgatcaaat ggtggggccc
      601 gctgatccag gaatattacg gcgccaccga aggccttggc ttcaccgcct gcagcagcgc
      661 cgagtggctg gcgcaccggg ggaccgtcgg caaggtcctg ctcggcgacc tgcacattct
      721 cgatgagaat atgcagccct gcccggtcgg tacacccggc acggtctggt tcaagaccgc
      781 cgcgcggttc gaatatttca acgatccgaa caagaccaaa gaggctcgct cgccggatgg
      841 cagcatgagc acggtcggcg acgtcggcta tgtcgacgcc gacggctttc tttatctcac
      901 cgatcgcgcg accttcatga tcatctccgg cggcgtcaac atctacccgc aggaatgcga
      961 gaacctcttg atcacccatc ccaagatcgc ggacgccgcc gtgttcgggg ttcccaatgc
     1021 cgacctcggc gaggaagtca aagcggtggt gcagccgatg ccggggattc ttcccggccc
     1081 ggatctcgtg gacgaactga tgttgttttg cagccaatcc ctgtcgcgcc aaaaagtgcc
     1141 gcgctcgatc gatttcgagg cggagctgcc gcggctgccg accggaaaac tctacaagcg
     1201 cctgctgcgc gaccgctact ggggcgacaa gacgtcacgg attttatgac cgttgagttt
     1261 cattcacttg ccgatcgcac aactgcccgg gttttcgcaa ggcgggggag ccgctcgcgt
     1321 gacggcggcg gcctcgatga aaggcgtgtc ttcaattccg ttcgcaacgg ccgagatgcg
     1381 ccagcgatag gcggtttgcg gcgtcaggcc ggaatcggcg aaactggcac ctgccacatc
     1441 tcccaacggc gcaaactgac cgtcggcgcc tgcgcgccgg acgcggtaaa ccgtcgttcc
     1501 cgcttgcggg gtccacgcca gatccgcgcc ggtgtcggag gcgtcgatca ccgtgagcga
     1561 ggttggcgcc acggcaggtg ccggcgcagg cacggcgtgc gccgtcaacg catcgagcat
     1621 ggccttgatc gtcctgatct gcgatccgga cttcgtgaca tagctgtcct cgtgatcgac
     1681 gtaactccac cagtcccagc atgcttccgg attgaacggc agcaacgaac tcgacgtggt
     1741 ctgcggataa agcacgatca gatgattggt gtctgcccag gaattgtacc cggtatcatc
     1801 gacgaaacgc cgaccgatat cgccggtatc ctgcttgcac ccatgaagcg cgatgtggac
     1861 gcggcacgcc tcgccatcct cgcatggcct gggaacgaac acgtagccgg cctcgcccag
     1921 gcttagcgtg ccgggatcat cgggcttggt ataaacagat tggtcgaagc gcttcgccgt
     1981 cccggtcaat tgtccccggt tgggggcgtt caacgcgccg taaatgtgct ggaggatgat
     2041 tccggcctgg tcatagccgc acgcatcgat aaacgggaca gcattgtcct tgcagtcgtt
     2101 gaggccgtcc ttgtgcggat cctgtgcaac gaccagcgaa tggccggcac cgatcgcggt
     2161 ctggtagtaa aggttgccgt ggttggtctc ggccagataa tgccggtaaa acgccgcggt
     2221 cgcatcggtg accggcctcg ccaccacggc atcgttgtag ccgtggaaga tatatacttt
     2281 ttgccggcgc acgaactgca gggaatcgat atcgccggag gcagatttgg cgtcggcctt
     2341 cgcgaaaaaa ctgctcaggt cggaaagcgg cccggacatg catgagcccg tcgcggtcat
     2401 gatcggaagc gtgaaggcgt tgatgaagtc gtcggcgtcc gccttggcgc accaaaacgg
     2461 gccgccggca acgacgccga cgcccttgat caccgaagac caagcggtgc cgaactggac
     2521 cgccatgaac gctccggatg agatgccgga taccgagctt tcgctgatgt tggcgttgta
     2581 gccggacagt cgcgccggct cttcagcgag tgccgggccc atgacccatg ccgtgcagcc
     2641 aacagcgatc gcagcacgat gcaacattcc tcggttcatg agacgctccc tgttgaactg
     2701 tgtcagcccg tgagatgcag acgtcatcgg cacgctgatt tcgtgccaag ccgcaaatgt
     2761 tgccgaacac cacggctcgg acgccgaccg tcaggcggca ccgcttcagt tcgttgccgg
     2821 ccggcaaatt ttaccgtaaa gttacgcaat aacaataaaa acagggagga cttgccgatg
     2881 gacgtcgtgc cgttgggtcc gggattcgcc gccgagttgc gcggggtgac gcttaaggat
     2941 gtcgcctcgg acgaggtcgc atatgcagca acacgcacgg cttttgagga acattccgtt
     3001 ctggtgttgc gcggccagga gatgaccgac gagcttcaac tggcgttttc gcgccgcttc
     3061 ggcccaccgg aagtgaccaa ggtcggctcg caggggacgg ggtcgcattt tgtgatcctg
     3121 agcaccatcg gccccgacgg caaggtggtg ccatcggatc accggcaggc gttgcgcaac
     3181 agggccaacc agctctggca taccgactcg tcgttcaagc gtgtgccggc gctcacctcg
     3241 atcctttcgg cgcgcatcat cccggcgcgc ggcggcgaaa ccgaattcgt ctcgacccga
     3301 cttgccttcg agcacctcga cgcggacctg cgcaacaggc tcgagaattc ctttgcctgg
     3361 cacgactacg cgcattctcg cggccagata tcggccgacc ttgcgagccc cgaggagcgc
     3421 gcggcgctgc cgccgcaatg ctggcgcatg gtctggaaga atcccgtgca cggtcgcggc
     3481 gcaatctatc tcgcctcgca tgcctatgcg gtcgaaggca tggaggcggc ggcggggaag
     3541 aagctgatcg acgaattgat ggcggccgcg accgcgcccg gcacgagcta cctgcatcaa
     3601 tggcgcggag gtgacgtcgt gatgtgggac aaccgcgcga ccatgcatcg cggacggccg
     3661 tggccggcgc acgaggcgcg gctgatggtc cggaccacga tctccgcgac cgaggccgat
     3721 ggcgttgcga ccatgcaccg gccctctccg caggccgcgg aatagcagcg aaaaaagcgg
     3781 acaccgcggc aagcctcagg tccgatttgc atcgaatgcg gaaagccgtt cagattcaac
     3841 ggcgggtttg ttagaagacc acccccagac gtcggccggc ggtccagacc acccggcagc
     3901 gtttcttggt gacgccgaac accaattcaa atcgattggg cagtcgggta ttgggatcta
     3961 cctcaaggca tgcgccgcca gcggaatagt caataatatt gcaatcaata atgggagctt
     4021 tcggaccaac gatgagtttg gccttcgagc tcttgcctgt cattcgaacg cgagcaaacc
     4081 gtcgcggata catcactcgt ccctatccat gacctgaaac ccattggata ggttcacaga
     4141 ctatcctgaa aatttcgttt caattttcca tacaaattcc gcggggcgcc gcgtccgcct
     4201 gagcagatac ggacgcccac agcaccatga taccgccgcc gggagtacgg attgatatcc
     4261 ggaggtttca gttggctgga atctctcgcg ggagatgatt ctcatgccgc agccagatga
     4321 ccgccgcgcc agaatgtgcc gcgacggaag cccggcatca ggctgccacc agccccgaaa
     4381 tctgtcgctg aagccacgcc agatattgct gcctgggttc gccttcctca gcaagctcca
     4441 gcagcgtctg gtgttcggtc ttgtcacgga cgatgaaggc aatcggtgcc gcaacacctc
     4501 cgatatgaag gctgcatgtc tcccgaaggc tcagatcagg aacgctcgcg atgcgcgctc
     4561 caccctgcga gatgtcgatc aatttggacg ccgttcgcga ttcccgtgaa ttcgtaacag
     4621 tcaggggcac gtctatccga aatcgaggga acacgcgtcg atcggcatct tctgacgatg
     4681 ttctcacgac ccgtacaagg atcgagcgca acgctgcaat gttcgccgat gcgttggcaa
     4741 tcgccccctg tacttcaacc gcgcgaatgc tcacttcttc ggcgccgccg ctgacgtttg
     4801 caattcgcga gctgacctcc cggctggatt gcgcggattg ctcgacgctg cgcgcgatct
     4861 ctcgcgtcgc ggcgccctgc tgttggatag ccgccgcgat cgaactggcg acacgatcga
     4921 cctctccgat ttcctgtccg atttgtccga ccgacgtggc cgccgcatcc gccgctgact
     4981 gtatttcagc caccagacga ttgatctctt cggtggattg cgcggtctga tgggaaagcg
     5041 acttcacttc cgacgccacg acggcgaaac ctcttccagc ctctccggcg cgggcagctt
     5101 caatggtcgc attcagcgcc agcagatttg tctggctg
//
LOCUS       DATNOI010000181         5472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_992365,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000181 DATNOI010000000
VERSION     DATNOI010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5472
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992365"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1719
                     /locus_tag="VN968_06955"
     CDS             <1..1719
                     /locus_tag="VN968_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HXL29012.1"
                     /translation="VWRIAAPYFRSEDKWAGRGLLVAVIAIELALVADDVLVNQWRNR
                     FYNAMQEKDWDGFVRETIIFTVLATASVVLSVYQLYLNQWLQIRWRNWLTTKYLGEWL
                     QGPNHYRMQLQGDAADNPDQRVSDDVKLFVDRTLNIGVALLSSIVTLFSFVIILWGLS
                     AAAPLHAFGVDFAIPGYLVWGALIYAIFGTALAQWIGSPLVNLDFQQQRLEADFRFSL
                     VRVRENSEQIALLRGEPAERQRLSERFGRVVGNWYAIMSRTKRLTVFTQSYGQAAIIF
                     PMILTAPAYFASKIQLGAVLQTAEAFGSVQTALSFFVSTYRSLAEWRAVVARLDGFET
                     AIASAATLASSASSIKMAPSTEGDEIDLQQLLVRLPNGTPLVSTDRLSLRTSERTLVT
                     GPSGAGKSTLFRAIAGIWPFGNGSIAIPANARLMMLPQRPYFPIGALQAAIVYPAEAS
                     AFSSDQVRDALIAVGMPQLATRLEEEAHWNRMLSLGEQQRLGLARALLHRPQYLFLDE
                     ATASLDEPSEAALYRLLEEKLPATTIVSIGHRSTLKAFHQRNVVLVRDGDQFALQSQS
                     EDAKHP"
     gene            complement(1745..2257)
                     /locus_tag="VN968_06960"
     CDS             complement(1745..2257)
                     /locus_tag="VN968_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HXL29013.1"
                     /translation="MRLDWLQAATDAIRRNDHRTVAAGAVAGLGAALAIGGMEWLSAA
                     SHFPLVIIPFATSIVLVIGSPEAEPAQPRALVGGHVMATLVGLAMLRLTGPHAWSAAA
                     AVGLAIVAMYATGTFHPPAGINPLLVVSNSLPWSFLFAPVLVGAVLLTAFAYVWHRWI
                     RRRPWPQRWL"
     gene            complement(2325..3317)
                     /locus_tag="VN968_06965"
     CDS             complement(2325..3317)
                     /locus_tag="VN968_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HXL29014.1"
                     /translation="MKKLVLAAVVLAMTAGSTFAADIAVKALKAPPPAPFEPWDVAFG
                     SAIMNDYIFRGVTQSNHKPSVAAYFEPRYNVTKDLQLYVGVSGESISFTNRAAAEIDV
                     YGGIRPTFGAFAFDIGVWGYLYPGGTCFFGGNGFDFAGHFQGTECATNFLPNGNTMKK
                     DVSFFEVYGKGNYTVNDNWQFGVTEFYTPSFLNSGAWGNYSSVTGKYTAPATIFGSSG
                     VGMYVSGEFGRQWFGTTDSFYGVAAFPNGIHYADYDTWNIGIGFTYKVFTLDLRYSDT
                     DLSKGNCSAFTGAFNASGNTNVTPINPGGTGSNWCGAAGIAKLSADLTAMTNLK"
     gene            complement(3748..4242)
                     /locus_tag="VN968_06970"
     CDS             complement(3748..4242)
                     /locus_tag="VN968_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29015.1"
                     /translation="MTEALDRRWTSDNRRITMAKGKKKKDKKSKKTKKKVLTAKKKTA
                     KKSSKKAAKKSAKKATKSAKKAAKKSKAAAPKKAAKKAGAKKRSAKKQAAPSITPPAP
                     EAVAPKPKPKPRAPKPAATPAPTPAPVPSWAAPSPAASSPAPSWGHSDNSSSPSSSDG
                     EDEN"
     gene            complement(4267..>5472)
                     /gene="glcF"
                     /locus_tag="VN968_06975"
     CDS             complement(4267..>5472)
                     /gene="glcF"
                     /locus_tag="VN968_06975"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HXL29016.1"
                     /translation="VLLGDELDSPRGRIYLIKEMLEKNRPPTQEVVKHIDRCLSCLAC
                     MTTCPSGVNYMHLVDQARARIEKDYRRPPTERLLRAVLAWVLPRPGLFRLSMRLARFG
                     RPLAALLPTSKAASASPTLWRRIRAMLALAPGRLPASGPSGGTVFPALGARRGRVALL
                     QGCAQQVLAPRINQAAINLLTRHGIEVVLVKDEQCCGALTHHLGRDGDALARARANIG
                     VWLEEAGRDGLDAILVTTSGCGTVIKDYGFMLREDRDFAGPAAEISALARDITEYLGG
                     IDLRRQQQQQSDIVVAYHSACSLQHGQKITQLPKELLSKSGFVVKDVPESHLCCGSAG
                     TYNILQPDIAGRLRDRKVANIAMVKPDVIAAGNIGCMVQIAGGTSVPVVHTIELLDWA
                     TGGPRPGLS"
BASE COUNT         1021 a         1733 c         1641 g         1077 t
ORIGIN      
        1 gtatggcgga tcgccgctcc ttatttccgc tccgaggaca aatgggccgg ccgcggcctg
       61 cttgtcgcag tgatcgccat tgagctggcg ctggtcgcag acgatgtgct ggtgaaccag
      121 tggcgcaatc gattctacaa cgccatgcag gaaaaagact gggacggttt tgtccgggaa
      181 accatcatct ttaccgtcct ggcaaccgcc tccgtcgtgc tttcggtcta ccagctctat
      241 ctcaatcagt ggcttcagat ccgctggcgc aactggttga ccaccaaata tctcggcgag
      301 tggcttcagg ggcccaacca ctatcggatg cagctgcagg gcgatgcggc cgataacccg
      361 gatcaacgtg tttccgacga cgtcaagctg ttcgtcgacc gcacgctgaa tatcggcgtc
      421 gccctgttga gctcgatcgt gacgctgttt tcctttgtca tcatcctgtg gggactgtct
      481 gcggcggcgc cgttgcatgc tttcggcgtt gacttcgcaa tccccggtta cctcgtatgg
      541 ggtgcgttga tctacgccat cttcggaacg gcgctggcgc agtggatcgg ctcgccgctg
      601 gtcaacctcg attttcaaca acagcgcctc gaggccgatt tccgtttcag cctggtgcgg
      661 gttcgcgaaa actccgaaca gatcgcgctg ttgcgcggcg agcccgccga acgccagcgg
      721 ctgtcggagc gcttcggccg cgtcgtcgga aactggtacg ccatcatgag ccggaccaag
      781 cggctgacgg tatttaccca aagctacggc caggccgcca tcatattccc catgattctc
      841 accgcgcccg cctatttcgc cagcaagatc cagctcggcg ccgtgctgca gaccgcggaa
      901 gcgtttggca gcgtgcaaac ggcattgtcg ttcttcgttt cgacctatcg ctcgctggcc
      961 gaatggcgcg ccgtcgttgc ccgtctcgac ggatttgaaa ccgcgatcgc cagcgccgcc
     1021 acgctggcga gcagcgccag ctcgatcaag atggcaccat cgaccgaagg cgatgaaatc
     1081 gacctgcaac agcttctcgt ccgcctgccc aatggcacgc cattggtctc caccgaccgt
     1141 ctgagccttc gcacaagtga gcgcaccctg gtcaccgggc cgtccggcgc cggcaaatcg
     1201 acgctgtttc gcgccatcgc cggtatctgg ccattcggaa acggctcgat tgcgatccct
     1261 gccaacgcca ggctgatgat gctgccgcag cggccgtatt ttccaatcgg agcgctacag
     1321 gctgcgattg tctatcctgc ggaagccagc gcgttcagtt cggatcaagt ccgcgacgcc
     1381 ctcatcgcgg tgggcatgcc tcagctcgcg acgcggctcg aggaggaagc gcactggaac
     1441 cgaatgcttt cgctcggcga acagcaacgc ctcggactcg cgcgcgcgct gctgcatagg
     1501 ccgcaatatc tgttcctcga cgaggcgacc gcgtcgctgg atgagccctc cgaggcggcg
     1561 ctgtatcgtc tgctcgagga gaaattgccg gcaaccacca tagtctcgat cggccatcgc
     1621 tccacgctca aggccttcca ccagcgcaac gtcgtgctgg tccgcgacgg cgaccagttc
     1681 gcgttgcaga gccaaagcga ggacgccaag catccgtgac gtcatgagcc ttgcgcgatc
     1741 ggctctacag ccagcgctgc ggccacggcc ggcgccgaat ccagcggtgc cagacatagg
     1801 caaacgcggt cagcagcacg gcgcccacaa gcactggcgc gaacaagaat gaccagggca
     1861 ggctgttgga aaccaccagc aggggattga tcccggccgg cggatgaaat gttccggtcg
     1921 catacatcgc aacaatggcc aatccaaccg cggccgcggc cgaccaggca tgcgggccgg
     1981 tcaaccgaag catcgcgagc ccgaccaggg tcgccatgac atgcccgccg accagagcgc
     2041 gcggctgcgc cggctccgcc tcgggcgatc cgatcacgag cacgatcgag gtcgcaaacg
     2101 ggatgatgac cagcggaaaa tgcgatgcgg ccgagagcca ctccatgccg ccaatcgcca
     2161 gagcggcccc aagacccgca acagctcccg ccgcgacagt ccggtgatcg ttgcggcgga
     2221 tggcatccgt cgccgcctgc agccagtcca gcctcattca gatcacccca aacaaaaggg
     2281 gcggcagatc gctctgccgc ccccaaaagt ctcgagagaa tgacttactt caggttggtc
     2341 atcgcggtca ggtcggccga gagcttggcg atgccagccg cgccgcacca gttcgaacct
     2401 gtcccgcccg gattgatcgg ggtgacgttg gtgtttccgg aagcgttgaa ggcacccgta
     2461 aaggcgctgc agttaccctt cgacaggtcg gtgtcggagt agcgcaggtc gagcgtgaat
     2521 accttgtagg taaaaccgat gccgatgttc caggtgtcgt aatcagcata atggatgcca
     2581 ttcgggaacg ccgcgacacc gtagaagctg tcggtggttc caaaccattg ccgcccgaac
     2641 tcaccggaga catacatgcc aacaccgctg ctgccgaaga tcgtagccgg ggcggtgtat
     2701 ttgccggtga ccgaagaata gttgccccaa gcgcccgagt tcaggaaact cggagtgtag
     2761 aactcggtca cgccgaactg ccagttgtca ttgacggtgt agttgccctt gccgtagacc
     2821 tcgaaaaagc tgacatcctt cttcatggtg ttgccgttgg gcaggaagtt ggttgcgcat
     2881 tccgtgcctt gaaaatgccc cgcgaaatca aacccgttac cgccgaagaa gcaggttcca
     2941 cccggataca ggtagcccca gacgccgata tcgaaggcaa aggcgccgaa ggtcgggcga
     3001 ataccgccat agacatcgat ttcagccgcg gcgcggttgg tgaacgaaat gctctcgccg
     3061 gagacgccga cgtagagctg caggtccttg gtaacattgt agcgcggctc gaaataggcc
     3121 gcgaccgacg gcttgtgatt ggactgggtg acgccgcgga agatgtagtc gttcatgatc
     3181 gcgctgccga aagcgacatc ccagggttca aacggtgccg gcggcggcgc cttcagggcc
     3241 ttcacggcga tatcggccgc aaaggtcgaa cccgcagtca tcgcaagtac tactgcggcc
     3301 aaaaccagtt tcttcatgac gatccccatc acatgtaaaa cagcccagtt ccgacgcccc
     3361 cgcggacaag cgataaccga ctgcaaccga atttctttaa cctgaccaca atttggaatg
     3421 cgcttgccga tcagtgaagc gaaaaagtca agcatctgcc gactttttag gcgttatggc
     3481 cgttccatga aatgttgtcg gtgtgtgttg cattccgaca acaattttga ccgattttta
     3541 ggtcgtctaa cgggcatttg ggagacaggc gcggcgaacg acggttcggt cgccatcgac
     3601 cgatcccacc gaatcaagtc gatcggggta atccgctcca ggttttttgg ctctaggttt
     3661 ggacgcgctc gcaagaaagg ccgcagcgac gggcgctgcg gcctttggcg tttggacgtg
     3721 acccgcgcgg taccgggtcg cggttggcta gttctcgtct tctccgtccg aagatgacgg
     3781 gctcgacgag ttgtccgagt gtccccatga cggtgcggga cttgaggcgg ccggactggg
     3841 agcggcccag cttgggaccg gagccggcgt gggggctggc gtagccgcag gcttgggcgc
     3901 cctcggcttg ggcttcggct tgggtgccac ggcttcgggg gcgggaggcg tgatcgaagg
     3961 agccgcctgc ttttttgccg aacgcttctt ggcgccggcc ttcttggccg ctttcttcgg
     4021 ggctgccgcc ttggactttt tggcggcttt cttcgcagac ttcgtagcct ttttggccga
     4081 tttcttggcg gccttcttcg aagatttctt cgcggttttc ttcttcgcag tcagaacctt
     4141 tttcttggtc ttcttactct tcttgtcctt cttctttttc cctttagcca tcgtgatcct
     4201 cctgttgtcg ctggtccaac gtctatcgag cgcttcagtc atccgcttcc gcgcggggcc
     4261 aatcgatcag ctcaatcctg gccggggacc gcctgtcgcc caatcgagaa gctcaatcgt
     4321 gtgcaccacg gggactgacg tgccgccggc aatctgcacc atgcatccaa tattgcccgc
     4381 ggcaatcacg tccggcttga ccatcgcaat gttggcgacc tttcggtcgc gcaatctgcc
     4441 cgcaatgtca ggctggagaa tgttgtacgt ccccgccgaa ccgcaacaca aatggctctc
     4501 gggtacatct ttcaccacga atccgctctt ggaaagcaat tctttcggaa gctgtgtgat
     4561 tttctgtcca tgctgcagcg aacaagccga gtgataggca acaacaatat cgctctgttg
     4621 ctgctgctga cgccgcagat cgatgccgcc gagatactcg gtgatgtccc tggccagcgc
     4681 cgaaatctcc gcggcggggc ctgcgaaatc gcggtcctcg cgcagcatga aaccataatc
     4741 cttgatcacc gtgccgcagc ccgacgtcgt caccaggatg gcgtcgagac cgtctcggcc
     4801 ggcttcctcg agccagacac cgatgttggc ccgcgcgcgc gccagcgcgt cgccatcgcg
     4861 tccgagatgg tgggtcagcg cgccgcaaca ttgctcgtcc ttgaccagca cgacctcgat
     4921 gccgtgacgc gtcagcagat tgatcgcggc ctgattgatt cgcggtgcca gcacctgctg
     4981 ggcgcagccc tgcagcagcg cgacgcgtcc gcgccttgcg cccagcgccg ggaaaaccgt
     5041 cccacctgac ggccctgatg ccggcaggcg gcccggcgcg agcgccagca tcgccctgat
     5101 tcgccgccat aaggtcggcg acgccgacgc agccttggag gtcggcagca aggcggcgag
     5161 cggccggccg aaccgtgcga gcctcatgct caggcgaaac aggccgggac gcggcagcac
     5221 ccatgccagt accgcacgca gcagccgctc ggtcggcggc ctccggtagt ctttttcgat
     5281 cctggcccgc gcctgatcga cgagatgcat gtagttcacg cccgacggac aggtggtcat
     5341 gcaggcaagg caggagaggc agcggtcgat gtgcttgacc acctcctgcg tcggcggccg
     5401 gttcttttcc agcatttcct tgatcagata gattcggccg cgcgggctat cgagctcgtc
     5461 gccgagcagc ac
//
LOCUS       DATNOI010000182        10803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_958064,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000182 DATNOI010000000
VERSION     DATNOI010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10803
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958064"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="VN968_06980"
     CDS             complement(<1..153)
                     /locus_tag="VN968_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HXL29017.1"
                     /translation="MTYCCGVLVRDGLVMIADTRTNAGLDNVSTFRKLHIFSKPGDRI
                     MAVASAG"
     gene            complement(549..1805)
                     /locus_tag="VN968_06985"
     CDS             complement(549..1805)
                     /locus_tag="VN968_06985"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HXL29018.1"
                     /translation="MKPASRINRATFFTSLVAVALLTSSARCDVIMDWNAKADAIGIE
                     KQLANVPNARGLAMLHIAMFEAVNAIDRRYAPYKLNLAAERTTSKEAAAASAAYDILV
                     ALYPDQKADLDATLAASLSGIAETEAKSKGIELGKKAAAGIIALRVNDSGDKTENYRP
                     YTTPGIYVPTALPIESTSPALTPWVMGTGSQFRPGPPPALSSETWTRDLNEIREIGSR
                     NSSKRTAEQTNIGRFWFQTGPRTYNPIVKQIAMARKMDVVDCARLFALSSIAGVDAFI
                     AVFDAKYTYNLWRPVTAIRNADLTSNAATPREESWTPLGVTPMHPEYPCAHCIVAAAI
                     STVLQSVAGDEVDAISLTSPTAPGVTRKWTRLQDYSDEVSNARIYAGFHYRFSTEVGK
                     DMGKKIGQLTVATQLRGAVASAQPKP"
     gene            1921..3492
                     /locus_tag="VN968_06990"
     CDS             1921..3492
                     /locus_tag="VN968_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXL29019.1"
                     /translation="MRADPQNQAAPLSYLAETKSYRFGRFVVDRRSACLRCDGVVLPL
                     RPKSFDVLVYLVQNPGRLVPKGELIDNVWQDVMVTDNSLVQCIKDIRQALNDNAHAEI
                     ETVAKRGYLFASPVVVIDGNAVDLGSPTLTAAAAGGDGALPLPDRPSIAVLPFANMSG
                     DSDQEYFADGISEDLITGLSRIRWLFVIARNSTFVYKHRAVDVKQVSRELGVRYVLEG
                     SVRRAGSRIRITAQLVDAITGGHHWAERYDRELGDIFAVQDEITSSVVAAIEPRLLAA
                     EGIRSLSRSAEDLGAWELVARAQTHVWRLTRLDYETAIDALKRTVEIHPDYAPARSLL
                     AFCLSFAAHMGWVNRDHGLLVGRQHAVRAVALDDCDSWGQIALGYLALMEKRTEESIA
                     AFRRAVLLNPSSAAAHGKSWPRPGICRARPRGDRACRGSDQAQSPGSGDGAVPRGCRG
                     SSLLCRSIRGSCRILVAALASTPRFSGGAAIALRGPRADRTDRGGPEISRNRTARATP
                     AIDRLDQSQRSLSDA"
     gene            complement(3584..4570)
                     /locus_tag="VN968_06995"
     CDS             complement(3584..4570)
                     /locus_tag="VN968_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL29020.1"
                     /translation="MNLHQSVPWIAAAALIALPFVYREPYHLHILILILIWSFAYTSW
                     SMMGRFGLVSLGHGGFMGLGAYVTALLWNHLGVSPWLGIPLAMIAAAVLAVIVAYPCF
                     RFRITGHYFVLVTLALSGIVLQVITATRDYTGGSLGYTPDRTKGSHLLALQFDDKITW
                     YLIALGVWALGLVIWRWVDRSMDRHALEAISEDEDAAAAAGVNVTWEKLKITVISALM
                     TSLAGALYCQYQMFISPDTVSGISVSLQMVFAVIVGGLYVSLGPTVGAVITILLAEIL
                     RIGFGTKAVGWDNLVYGVLLVVFIIFLPKGILGSILEKLKTQRQRQVRRIND"
     gene            complement(4567..5439)
                     /locus_tag="VN968_07000"
     CDS             complement(4567..5439)
                     /locus_tag="VN968_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL29021.1"
                     /translation="MTDIFDIYLLEAVVNGILLGGLLALLALGLNLIFGVIDVTWICY
                     AELVMIGMYGMYYLVQVFGLPYYVAAPFTILLVAGLGALLHLLVIAPLLSAPPINQLL
                     ATGGVLFVLQSFATVAFGIDFRNLGIRMPVLKFAEMHFSYSRLLAFAAALIGMLLVYF
                     FMKRTYVGTAIRAISQDRQIMALMGVDTRRLYLITSALGGALAGLASCLLVLQYDIHP
                     FVGLSFGPITFLICVLGGLGNFVGGFVAAFVFAEIISLGGLFSDLEWGYVLAFAFFIV
                     MMFIRPAGLLARRS"
     gene            complement(5463..6167)
                     /locus_tag="VN968_07005"
     CDS             complement(5463..6167)
                     /locus_tag="VN968_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL29022.1"
                     /translation="MLELKSVDAGYGTFQALFGVSLDVRAGEAVGVIGPNGAGKTTLM
                     RVISGLIRPRAGAISMEGTDVLRTPEHRIVSLGIAHVPENRRLFPRLTVQDNLKMGAF
                     MPEARARYAERLAFVFDLFPRMKERRNQLAGTMSGGEQQMCAIGRALMSDPKLLLLDE
                     PSAGLAPVVVQQVFELVKRIRANGLTVLIVEQNVQQVLRVVDRAYLMEAGSIRASGKS
                     SEMLANDTIRQAYLGM"
     gene            complement(6181..6936)
                     /locus_tag="VN968_07010"
     CDS             complement(6181..6936)
                     /locus_tag="VN968_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL29023.1"
                     /translation="MLVVEGLVKRFGGFTAVNKVSFKVDQGEILGLIGPNGSGKSTIF
                     NMLSGTLSPSAGSIKFAGTEIAGLAPHRIINRGIGRTFQIPRPFHRLTMFDNVALAGY
                     FGQGRHSRAKAEDAAERALAMVGLPADRTISVDGLGAAGLKKLELAKALATGPKLLLA
                     DESLGGLDDTEMDQAADMLRKIRDELGITIIWVEHIMGVLMRVVDRVMVLDHGEKISD
                     GLPSEVSNDPRVVEVYLGTDADATQAAAAEARR"
     gene            complement(6966..8276)
                     /locus_tag="VN968_07015"
     CDS             complement(6966..8276)
                     /locus_tag="VN968_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL29024.1"
                     /translation="MLVRKAVRLLGGLVVAAVALGMAVSAQAQEKKIKIGVIYDLTGP
                     LAGGGSELQYIGAKIMLDQYAKTGVEGYKVEAIYADAQSKPDVAINEAVRLVEQEKVD
                     MLLGFFSSAQCVPVAARVEQLKKFMWITTCISSSVLDNKNFKYVFRPQASGDQFGMMT
                     MDFIAQNSQSKFGKEPKDLRVAIIHEDGAYGVDVAKGNVAGAKKAGFNVVLKEGYSAT
                     APDLSALVTKLKRAQPDVIFHTGYNPDITLLLRQTREQGLKFGALVGHGAGYGVYEKL
                     KEGLGPDANYIFNTDPISIWLANQKSMDPKLPPIIKMVGEEFDKVKPGVAIRSAHVGM
                     AASNTYVFLTEVLPRAIKKYGGVDPDALRKAALEVDLPEGGTMLGFGVKFYGEGTQMA
                     GQNERSFPVVIQYVDDKSYVVWPKSQAQREAVLPLPAGTTYSYK"
     gene            complement(8705..9220)
                     /locus_tag="VN968_07020"
     CDS             complement(8705..9220)
                     /locus_tag="VN968_07020"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXL29025.1"
                     /translation="MDIATFRTTQEPFKELYRKDAKAAFLTLRAKGTADESAVTCKVE
                     TGRGLALAGIHPKAGGTGRELCSGDMLLEALIACAGVSMRAAAAVLDIPIKSAVVSAE
                     GDVDLRGTLGVTEGVPVGFKAIRLRFDVKTDAPQNQLDQLLELTDRYCVIFQTIQNSP
                     KTTVKLNRVGL"
     gene            complement(9287..9712)
                     /locus_tag="VN968_07025"
     CDS             complement(9287..9712)
                     /locus_tag="VN968_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXL29026.1"
                     /translation="MTVNKGRCFCGAIEIEVHGAPEAMGYCHCNSCRSWSASPVNAFT
                     LWKPQNVEITKGAEFLSRFMKTEASGRQFCKRCGGHLMTDHPQLGLVDVYAATIPTLK
                     FSPGVHVNYAETVLPMKDGLPKLKDFPAELGGSGVPVPE"
     gene            9893..>10803
                     /locus_tag="VN968_07030"
     CDS             9893..>10803
                     /locus_tag="VN968_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXL29027.1"
                     /translation="MAEDALSNLLKTVRLTGATFFDIEAQDPWAVRSPAPASILPRIL
                     PGADHLISYHVVTAGRCFARIVGGQPIVVEAGEVVVFTHSDPHIMSSSPGLRADPPAA
                     DLLDIAFAGQMPFHINYVSGGPISAKLVCGYLACDAQPFNPLLEALPPVIKVGNPRDG
                     DAGWLGQFIRFAVAEVAEKRAGGETVLTKLSELMFIDVPRRYIETLPPQNTGWLAGMR
                     DPLVGKALSLIHDQPARNWTIETLAKQSGLSRTILAERFAKLVGIPPMHYLAKWRMQI
                     ASELLSGSNANIASIAAKTGYESDAAF"
BASE COUNT         2076 a         3318 c         3316 g         2093 t
ORIGIN      
        1 gccggcgctc gcgaccgcca tgatgcggtc gcccggtttg ctgaagatat ggagtttgcg
       61 aaaggtcgag acgttgtcga ggccggcatt ggtacgggtg tccgcgatca tgaccagacc
      121 gtcccgcacc agcactccgc agcaataggt cattccaatc cccgaattct tcagcgcgcg
      181 aaattactaa ccaatttcgc aagcgcctcc aaccccattc gggggcatcc gcgcaggttt
      241 ggggcgattc cgtcgtggcg ttgccgatat cgaccggtta gacgattttc cgtcgaaacc
      301 tgagggcagg gcggcgcatg aaaaagcaaa tgaatatcgg cggtctggtg gtggccgcgc
      361 aaatcttggg cattccgggc gctttcgcag gccccctggg aagacggcat cgcggcccat
      421 aagcgcggcg actacgtgcc ggagttgcga gtatcgatta aaaccgtcgc tgtaagatgc
      481 gccaaattgg cgcgttttgc agcgttcacc gcagagaaac gcctctgata tgtaaaagcc
      541 ttctcacgtc agggtttcgg ctgcgcggag gcaacggcgc cacgcaactg cgttgccacc
      601 gtcaactgcc cgattttctt ccccatgtct tttccaacct cggttgagaa gcggtaatga
      661 aagccggcgt aaatgcgggc gttcgagacc tcatcactgt agtcctgcag gcgcgtccat
      721 ttgcgcgtga ctccaggagc agtcgggctc gtcagcgaga tagcatcgac ctcatcccct
      781 gcgacgcttt gaagaacggt ggaaatagcg gctgcgacta tgcagtgcgc gcacggatat
      841 tcgggatgca tcggggtaac gccgagcggc gtccatgact cttcgcgcgg cgttgcggca
      901 ttggatgtga gatcggcatt gcggatcgcc gtcaccggcc gccatagatt gtaggtatat
      961 ttggcgtcga acacggcgat gaaggcgtca acgcccgcga tcgaactgag cgcgaacaga
     1021 cgggcgcagt caacgacgtc cattttccgg gccatcgcga tctgcttgac gatcggattg
     1081 taggttcggg gtccagtctg aaaccagaag cggccgatat tggtctgctc agccgtgcgc
     1141 ttgctgctgt tgcggcttcc gatctcgcgg atctcgttca aatccctggt ccacgtctcc
     1201 gaactaagcg ctggaggagg gcccggacgg aactgcgagc ccgtgcccat cacccacggt
     1261 gtcagcgccg gactggtgga ttcgatcggc agcgcggtcg gcacgtagat gcccggcgtc
     1321 gtgtagggcc ggtaattttc cgtcttgtcg ccggagtcgt tgacgcgcag cgcgataatt
     1381 ccagccgccg cttttttgcc aagttcgatc cccttcgact tcgcctccgt ttcggcaatg
     1441 cccgaaagcg aagccgcgag cgtcgcgtcg aggtcggctt tctgatcggg ataaagcgcc
     1501 accagaatat cataggcggc ggaagcagcc gccgcttcct tggaagtcgt gcgttctgcc
     1561 gcgaggttga gcttgtaagg ggcgtatcgt cggtcgatcg cgttgaccgc ttcaaacatc
     1621 gcaatatgga gcatcgccag tccgcgcgca ttcgggacat tggcaagttg cttctcgatg
     1681 ccgattgcgt cggccttggc gttccagtcc atgatgacgt cgcagcgcgc ggaactggtc
     1741 agcagcgcca ccgcgacgag actggtgaag aacgtcgctc ggttgatcct tgacgcgggt
     1801 ttcatgggca aactccttca cggcgtgcca ggcacgacct ccgggattgg taggccgatc
     1861 ttgccgatct gcggcggggt gtcgtgaagg cctgatgaaa ttctgctgaa atggtctagg
     1921 atgcgggcgg atccgcaaaa ccaggctgca cccctgtcat atctggctga aaccaaaagc
     1981 tatcgtttcg ggcggtttgt cgtcgatcgc cgatccgcct gtttgcgttg cgatggcgtg
     2041 gttttacccc tgcggccgaa gtccttcgac gtgctggttt atctggtaca gaacccgggc
     2101 cgcctcgttc cgaaaggaga attgatcgat aacgtctggc aggacgtcat ggtcaccgac
     2161 aactcactgg ttcagtgcat caaggacatc cgtcaggcgc tcaacgacaa cgcccatgcg
     2221 gaaatcgaaa ctgtcgccaa acgcggctat ctttttgcat cgccggtggt tgtgatcgat
     2281 ggcaatgctg tggacctcgg ctcaccaact ctaacggctg cggcggctgg cggggacggc
     2341 gccctgccgt tgcccgatcg tccttccatt gcggtattgc cattcgccaa tatgagcggc
     2401 gattccgatc aggagtactt tgccgatggc atttccgagg atttgattac cggactgtcc
     2461 cgcattcgct ggctgttcgt catcgcccgc aattcgacat tcgtctacaa gcaccgcgcg
     2521 gtcgacgtga aacaggtttc gcgcgagctt ggggtgcgct acgtgctgga aggcagcgtg
     2581 cgccgcgccg gctcgcggat acgcatcacc gcacagttag tcgacgcgat aaccggcggt
     2641 catcattggg cggagcgcta tgaccgcgaa ctgggcgata tcttcgccgt ccaggatgaa
     2701 atcacctcca gcgtcgtcgc ggctatcgag cctcgtctgc ttgcagcgga gggcatccgc
     2761 tccctttcgc ggtccgcgga agatctcggc gcctgggaac tcgtcgctcg cgcccaaacg
     2821 catgtctggc gcctgacacg cctggattac gagaccgcga tcgacgctct caaacggacc
     2881 gttgagatac atccggacta cgcgcctgct cgaagcttgc tggcgttttg cctctcgttt
     2941 gctgcgcata tggggtgggt caaccgcgat cacgggttgc tcgttggtcg ccaacacgct
     3001 gttcgcgcgg tcgccctcga cgattgcgat tcctggggac agatcgcgct tggctatttg
     3061 gccctcatgg aaaaacgcac cgaggaatcg atcgcggctt ttcgtcgggc ggttctgctc
     3121 aacccgagtt ccgcggcggc ccacggcaaa tcttggccac ggcctggcat ttgccgggcg
     3181 cgaccgcgag gcgatagagc atgccgagga agcgatcagg ctcagtcccc tggatccgga
     3241 gatggcgctg ttcctcgggg ctgtcgcggt agctcattat tgtgccggtc gatacgcgga
     3301 agctgtcgaa tactcgtcgc agctcttgcg tctacgccca ggttttcagg gggcgcagcg
     3361 attgcgctgc gcgggcctcg cgcagaccgg acggatcggg gaggcccgga aatttctcgc
     3421 aaccgtacgg ctcgagcaac cccagctatc gatcgactgg atcagagcca gcgttcccta
     3481 tcagacgcct gaactgatgg agcggttcct ggacggcatg cgaaaggcag gattgaccga
     3541 gattgagagc aaaggcggct agcctgcgtc gctggattct atgtcaatca ttgatgcgcc
     3601 gaacctggcg ctggcgttgc gttttcagct tctcgaggat gctgcccaaa attcccttgg
     3661 gcaggaagat gatgaagacc accagcagca cgccgtagac cagattgtcc cagccaacgg
     3721 ccttcgttcc aaagccgata cgcaatattt ccgccagcag aatggtgata acagcgccga
     3781 ccgttggtcc gagcgacaca tagaggccgc caacgatgac ggcaaagacc atctgcaagg
     3841 ataccgaaat gccgctcacg gtgtcggggg agatgaacat ctggtactgg cagtagagcg
     3901 cgccggcgag tgacgtcatc agcgcgctga tcacggtgat cttgagcttt tcccaggtca
     3961 cgttgacgcc ggccgcagcg gctgcatcct catcctcgga gatcgcctcc agggcatggc
     4021 ggtccatgct gcggtcgacc cagcgccaga tcacgaggcc aagcgcccag accccgagcg
     4081 cgatcagata ccaggtgatc ttgtcgtcga actgcagcgc cagcagatgg ctgcccttgg
     4141 tccggtcggg cgtgtaccca agcgagccgc cggtgtagtc gcgcgtggcg gtgatgacct
     4201 ggaggacgat tccggacagc gccagcgtga ccagcacgaa atagtggccg gtgatgcgga
     4261 agcggaagca aggatacgcc acgatcaccg ccagcaccgc cgcggcgatc atcgccaggg
     4321 gaatgcccag ccacggcgac acaccgaggt ggttccacag cagcgccgtc acataggcgc
     4381 cgaggcccat gaacccgcca tgcccaagtg aaaccaggcc gaagcggccc atcatcgacc
     4441 acgacgtgta ggcgaacgac cagatcagga tcaggatcag aatgtgcaga tggtagggtt
     4501 cgcgatagac gaagggcagg gcgatgagcg ctgcggcggc aatccagggc accgactgat
     4561 gcaggttcac gagcgcctcg ccagcagccc ggcgggcctg atgaacatca tgacgatgaa
     4621 gaaggcgaag gccagcacgt agccccattc cagatcggaa aacagcccgc cgagcgagat
     4681 gatctcggcg aatacaaacg cggcgacgaa gccgccgaca aaattgccga gcccgcccag
     4741 cacgcaaatc aggaaggtga tcggtccgaa cgacagtccg acaaatgggt ggatgtcgta
     4801 ttgcaagacc agcaggcagg acgcgaggcc ggctagcgcg ccgcccagcg ctgacgtgat
     4861 gagatagaga cggcgcgtgt cgacgcccat cagcgccatg atctgccgat cctgggagat
     4921 tgcgcggatg gcggtgccga cataggtccg cttcatgaag aaatagacca gcagcatgcc
     4981 gatcagcgcg gcggcgaaag cgagcaggcg tgaataactg aaatgcatct cggcgaactt
     5041 caacaccggc atccggatgc cgaggttacg gaagtcgatg ccgaacgcga cggtggcgaa
     5101 gctttgcagc acgaacagca cgccgccggt ggcgagcagc tggttgatcg gcggcgccga
     5161 cagcagcggc gcgatcacca gcaaatgcag cagcgctccc aggcctgcca ccagcaggat
     5221 ggtgaagggg gccgcaacgt aatagggcag cccgaaaacc tggaccagat agtacatgcc
     5281 atacatgccg atcatgacaa gttcggcgta acagatccac gtcacgtcga tgacgccgaa
     5341 gatcaggttc agcccaagcg ccagcaacgc cagcaggccg ccgagcagaa tgccgttcac
     5401 gacagcttcg agcaggtaga tgtcgaaaat atccgtcatg gttttatcta gactccgaaa
     5461 tcttacatcc caagataggc ttgccggatg gtgtcgttgg ccaacatctc gctcgacttg
     5521 cccgaggcgc ggattgagcc tgcctccatc aggtaggcgc gatccaccac gcgcaggacc
     5581 tgctgtacat tctgctcgac gatcagcacg gtcaggccat tggcgcggat tcgcttcacc
     5641 agttcgaaaa cctgctgaac cacgacaggc gcgagaccgg ccgagggttc gtcgagcagc
     5701 agcagcttcg gatccgacat cagcgcgcgg ccgatggcgc acatctgctg ctcgcctcct
     5761 gacatggtgc cggcgagctg gttgcggcgt tccttcatgc gtggaaacag atcaaacaca
     5821 aacgccagcc gttcggcgta gcgggcccgc gcttcgggca tgaaggcgcc catcttgagg
     5881 ttgtcctgca ccgtcagacg aggaaatagc cggcggttct ccggcacgtg ggcgatgccg
     5941 agactgacga tgcgatgctc cggcgtcctc aggacgtcgg tgccctccat tgaaatcgct
     6001 cccgcgcgag gccggatcag accggagatc acccgcatca aggtggtctt gccggcgccg
     6061 ttggggccaa tcacgcccac cgcttcgccc gccctgacgt cgagactgac gccgaacagc
     6121 gcctggaagg ttccgtagcc cgcatccacc gatttcagtt cgagcatgcg tgcaccccgc
     6181 ctaacgacgc gcttcggccg cggcggcctg ggtcgcatcg gcatcggtgc cgagataaac
     6241 ctcgacgacc cgcggatcgt tcgacacctc gctcggcagg ccatcggaaa tcttctcgcc
     6301 gtgatccagc accatcaccc gatcaaccac gcgcatcagc acgcccatga tgtgctcgac
     6361 ccagatgatg gtgatgccaa gctcgtcgcg aatcttgcgc agcatgtcgg cggcctggtc
     6421 catctcggtg tcatcgagtc cgcccaggct ttcgtccgcc agcagcaatt tcggcccggt
     6481 cgcaagcgcc ttggccagct ccagcttctt caggccggcg gcgcccagcc cgtcgacact
     6541 gatggtgcgg tccgccggca ggccgaccat ggccagcgcg cgttccgcgg cgtcctcggc
     6601 tttcgcacgg ctgtgccggc cctgcccgaa gtagccagcc agcgccacat tgtcgaacat
     6661 cgtcagccgg tgaaacggtc gcgggatctg gaaggtccgg ccgatgccgc gattgatgat
     6721 ccggtgcggc gcgagacccg cgatctccgt cccggcgaat ttaatcgaac ccgccgacgg
     6781 cgaaagcgtg ccggagagca tgttgaagat cgtgctcttg cccgagccgt tcgggccgat
     6841 caggccgagg atctcgccct gatcgacctt gaacgatacc ttgttgacgg ccgtgaagcc
     6901 gccaaaccgc ttgaccaagc cctcgactac cagcacggcg gtcccttccg tcgatccagg
     6961 accacctatt tgtagctgta ggtcgtgccc gccggcagcg gcagcacggc ctcgcgttgc
     7021 gcctggctct tcggccacac cacatacgac ttgtcgtcga cgtactggat caccacaggg
     7081 aacgagcgtt cattctgtcc ggccatctgg gtgccttcgc cgtagaactt gacgccgaag
     7141 cccagcatcg tgccgccctc gggaaggtcg acttcgagcg ccgccttgcg cagcgcgtcg
     7201 gggtcgacgc caccatattt cttgatggcg cgcggcagca cctcggtcag gaacacgtaa
     7261 gtgttcgaag ccgccatgcc gacatgcgcc gatcgtatgg cgacgcccgg tttcaccttg
     7321 tcgaattcct cgccgaccat cttgatgatc ggcggcagct tcggatccat tgatttctgg
     7381 ttcgcgagcc agatcgagat cggatcggtg ttgaagatgt agttcgcgtc ggggccgagg
     7441 ccttccttga gcttctcgta tacgccgtag cccgcaccgt ggccgaccag ggccccgaac
     7501 ttcagcccct gttcgcgcgt ctggcgaagc agcagcgtga tgtcgggatt gtagccggta
     7561 tggaagatga catctggttg agcgcgcttc agcttggtga ccagcgcaga gagatcgggc
     7621 gcggtagccg aatagccttc cttcagcacc acgttgaagc cggccttctt ggcgccggca
     7681 acgttgcctt tggccacgtc aaccccgtag gcgccatcct cgtgaatgat ggcgacgcgc
     7741 aggtccttcg gctccttgcc gaacttggac tgcgagttct gggcgatgaa gtccatcgtc
     7801 atcatgccga actggtcgcc gctcgcctgc ggacggaaga catacttgaa attcttgttg
     7861 tcgagcaccg acgatgaaat gcaggtggtg atccacatga acttcttgag ctgctcgacg
     7921 cgggcggcca ccggcacgca ctgcgccgac gagaagaagc ccaagagcat gtcgaccttt
     7981 tcctgctcga ccaggcggac cgcctcgttg atggcgacgt caggcttgct ctgggcgtcg
     8041 gcgtagatcg cctcgacctt gtaaccttcc acaccggtct tggcgtactg gtcgagcatg
     8101 atcttggcgc cgatgtactg cagctccgaa ccgccacccg cgagcgggcc ggtcaggtcg
     8161 tagatcacgc cgatcttgat tttcttttcc tgggcctggg ccgaaaccgc catgcccagc
     8221 gccaccgccg ccacaaccag cccgccgagc aggcggacag ccttacgcac cagcatcaaa
     8281 acctccctgg aaaattgtgc attgcgtttt tgtttttgtt gacgcccatc acatggggag
     8341 cgccgaccga tgtcaagcgc ctagcgacgc cgacccgacg ccagggcgtc gcaggtcatg
     8401 ccaattgttt gagcgtgccg aaccgcgcgt cgggaaatgt tgccatgcgc gtgcttacgc
     8461 gcgagcgcgg cgggcgtcta cctcacggcg agggctggat tgcgcgaggg gtcgaattac
     8521 cggagttgcg caatattgat tgagccgtcg ccctcagacg cgccaattgg cgcgtctgag
     8581 ggcgagcgaa ccggttatcc gggactccgg cctgtgaaat gccgccgcac cttgtcgcgc
     8641 ggcggcattt caccgtcatg aatttgcgtc gcggctcaag ccggtggagc gcgaaccaac
     8701 acccttacag gccaaccctg tttaacttta ccgtggtttt tgggctgttc tggatcgtct
     8761 gaaagatcac gcaatagcga tcggtgagtt cgagcagctg atcgagctgg ttctgcggtg
     8821 cgtcggtctt gacgtcgaag cgcaagcgga tcgccttgaa gccgacgggc acgccctcgg
     8881 tcacgccgag tgtgccgcgc agatcgacgt cgccttcggc tgaaaccacc gctgacttga
     8941 ttggaatatc gagcacagcc gccgccgctc tcatcgagac gccggcgcaa gcgatcaggg
     9001 cttcaagcag catgtcgccc gagcagagct cccggccggt gccaccggcc ttcgggtgga
     9061 tacccgcgag cgcgagtccg cgtccggtct cgaccttgca ggtcacggcg gattcgtcgg
     9121 cagtaccctt ggccctcaag gtgagaaacg cggccttggc atcctttcgg tagagctcct
     9181 tgaatggctc ctgtgtcgtt cggaacgtcg caatatccat agatcttctc ctcgactggt
     9241 ttggtgttga tcgggcgcgc gagcgatgag tcctcccgcg ttggcgctac tccggcacgg
     9301 gcacgccaga acctccgagc tcggcgggaa agtcctttag tttcggtagt ccgtccttca
     9361 tcggcaggac tgtctcggcg tagttgacgt ggacaccggg gctgaacttc agggtaggta
     9421 tcgtggccgc atagacgtcc accaaaccca actgcgggtg gtcggtcatc agatggccgc
     9481 cgcatcgctt gcaaaactgg cgaccgctcg cctctgtttt catgaaccgg gatagaaact
     9541 ccgcaccttt tgtgatttcg acgttctgcg gcttccacaa ggtgaaggcg ttcacgggtg
     9601 acgccgacca ggatcggcac gaattgcaat ggcaataacc catcgcctcg ggagctccgt
     9661 gcacttcgat ttctatggcg ccacagaaac atcggccttt gtttactgtc atgaccttgc
     9721 aactcctgat tcttgctcat tccgagcgcg cccaccgcga gcgatttaaa cgttccggcg
     9781 gcgaactgaa tggcagggcg ttcgggttac ttgacagagc gtccggattt cgaaagggct
     9841 gtcgcagaag gcatccgcga tcgatgccac gccacttgcc aggcagagcc gaatggccga
     9901 ggacgctcta tccaatttat tgaagactgt gcggctgacc ggcgcgacgt tctttgacat
     9961 cgaggctcag gacccatggg ctgtccggtc gccggcaccg gcttcgatct tgccgagaat
    10021 tcttccgggc gccgaccatt tgatttccta tcatgtggtg acagccggcc ggtgttttgc
    10081 gcgtattgtc ggtgggcaac cgattgtcgt cgaagccggc gaagtcgttg tcttcaccca
    10141 cagcgatccg catatcatgt cgagcagccc cggcctccgc gccgatccac ccgcggcaga
    10201 cctgctcgac atcgcctttg ccggtcaaat gccgttccat atcaactacg tcagtggcgg
    10261 accgatctca gccaagctcg tctgcggata tctcgcctgc gatgcgcagc cgtttaatcc
    10321 gttgctcgag gcgttgccgc ccgtgatcaa ggtgggaaac ccgcgcgatg gcgatgccgg
    10381 ctggctcggc cagttcatca gatttgcagt ggcggaagtg gcggagaagc gcgccggcgg
    10441 tgagacggtg ctaaccaaac tgagcgagtt gatgttcatc gacgttccgc gccgctacat
    10501 tgaaacgctg ccgccgcaga acaccggctg gcttgcgggc atgcgtgatc cgctcgtagg
    10561 caaggcgctg tcgctgatcc acgatcagcc ggcgcgcaac tggacgatag agacgctggc
    10621 gaagcagtct ggactgtctc gcaccatcct ggccgaacgc tttgcgaagc ttgtcgggat
    10681 tccgccgatg cactatctcg ccaaatggcg gatgcagatt gcttcggagc ttttgagcgg
    10741 cagcaacgcc aacattgcca gcatcgcggc gaaaaccggc tacgaatccg acgcggcatt
    10801 cag
//
LOCUS       DATNOI010000183         7963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_91293,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000183 DATNOI010000000
VERSION     DATNOI010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91293"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VN968_07035"
     CDS             complement(<1..414)
                     /locus_tag="VN968_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HXL29028.1"
                     /translation="MMRKTSLILLSAATGAALTLFVTQPRAVMMGSSARAATSDTYRQ
                     LNLFGDVFERVRSDYVEKPDDSKLVESAISGMLTGLDPHSSYMDAKSFRDMQVQTRGE
                     FGGLGIEVTMEDGLIKVVSPIDDTPASKAGIMANDI"
     gene            complement(411..1712)
                     /locus_tag="VN968_07040"
     CDS             complement(411..1712)
                     /locus_tag="VN968_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXL29029.1"
                     /translation="MPAAAQALPTPAQVLPAAQDAASTQQTTAASPDAIKQREQELEA
                     ARAQQKSAAELQLKLQADLAAIGQDRSKLNQRLIDIAGQVRSVEARIGDAETRLRPLD
                     SREQQIRGSLGSRRSEIVEVLATLQRAGRRMPPALLVRPEDALQSLRTAMLLGAVVPE
                     LRGRADRLATDLGELVSVRKSIASERDQLAVDRDKLRDDQTRLAALVDERQRKQNSIE
                     KDMEAESARAIALSQQADGLEGLIAKMEQDLKSAARAAASASLRGAPVAPNGKPNLGG
                     LKDSARLSPAIAFASAKGLFALPVNGVKIREFGGSDGSGGVEKGISLSTRAGAQVTTP
                     CDGWVVYAGPFRSYGQLLILNAGGGYHVLIAGMERISVNIGQFVLTGEPVATMGTKSQ
                     VASILAANASQPVLYVEFRKDGTPIDPGPWWAANEGEKVRG"
     gene            complement(1939..2421)
                     /gene="rlmH"
                     /locus_tag="VN968_07045"
     CDS             complement(1939..2421)
                     /gene="rlmH"
                     /locus_tag="VN968_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HXL29030.1"
                     /translation="MRLVVISIGRLKQGPERELAERYRERFDDIGRKLGFRGLEIQEI
                     PESRARDAATRMTDEAAAISAAIPTKSVLVALDERGESIDSAAFARHLGRWRDEQVAN
                     TIFVIGGADGLSPDLRRKAKLNIAFGSATWPHQMVRVMLLEQVYRAATILAGHPYHRV
                     "
     gene            complement(2672..3028)
                     /gene="rsfS"
                     /locus_tag="VN968_07050"
     CDS             complement(2672..3028)
                     /gene="rsfS"
                     /locus_tag="VN968_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HXL29031.1"
                     /translation="MKAQPDADKTLNMILSRLDDMKAEETVTIDLRGKSAFSDYMIVT
                     SGRANRHVGAIAENVTKGLKETGIKSIHIEGLPNCDWVLIDSGDVIVHVFRPEVREFY
                     NLERLWTQSPTAAKAI"
     gene            complement(3224..3871)
                     /locus_tag="VN968_07055"
     CDS             complement(3224..3871)
                     /locus_tag="VN968_07055"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HXL29032.1"
                     /translation="MALPSISQAIPLYTNGMRIGLLGGSFNPPHVAHRAISLFAIKRL
                     KLDRVWWLVTPGNPLKDQRALHDLEERMIAARKMANDPRIDVSCLESVIRTRYTVDTI
                     TYLRRRASGLRFVWIMGADNLAQFHRWQNWRRIAAEAPIAVIDRPPQSFRALAAPAAQ
                     ALAHYRLPENQAGRLADQRAPAWVFLTGMKLNLSSTGLRNPDGSWRTQRGQAGVK"
     gene            complement(3961..5253)
                     /locus_tag="VN968_07060"
     CDS             complement(3961..5253)
                     /locus_tag="VN968_07060"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HXL29033.1"
                     /translation="MSAPLKAIDGNADLPVLMTGLAIHARAAARVLALASPEQKNHAL
                     DAIERAIRANASAILGANAEDVAEVRAGGASSAFVDRLTLTQARIDAMADGVATVRDI
                     ADPIGVVTESWQRPNGMIIERVRVPLGVVAVIFESRPNVAADAGVLCLKSGNAVILRG
                     GSDSFRSCRAIHDCLVQGLREAGLPEASITLVPTRDRAAVGLLLAGLNGGVDVIVPRG
                     GKSLVARVEAEARVPVFAHLEGVNHVYVDHAANLEMAKSIVLNAKMRRPGVCGAAETL
                     LVDRKGASTNLKPLVAMLIDAGCEVRGDDAVQHADPRVQPASDEDWDTEYEDAIITAK
                     VVDGVDEAIAHIHNHGSHHTDAIVTEDAGAARKFLNEVDSAIVLHNASTQFADGGEFG
                     FGAEIGIATGKFHARGPVGAEQLTSFKYRVHGTGQTRP"
     gene            complement(5408..6550)
                     /gene="proB"
                     /locus_tag="VN968_07065"
     CDS             complement(5408..6550)
                     /gene="proB"
                     /locus_tag="VN968_07065"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HXL29034.1"
                     /translation="MARPELKNFRRIVVKVGSSLLIDSDAGEVRAAWLAALAADLAKL
                     HGEGRELLIVSSGSIALGRSRLKLPRGTLKLEESQGAAAVGQIALARIWSEVLGHHGI
                     GAGQILVTLQDTEERRRYLNARSTIAKLLEWRAVPVINENDTVATTEIRYGDNDRLAA
                     RVATMTSADLLILLSDIDGLYDAPPAGNPNARLIAIVETVTSQIEAMAGAAESELSRG
                     GMRTKIEAAKIATTSGTHMLIASGKIEHPLQAIADGGRCTWFLTPANPVTARKRWIAG
                     SLEPRGTLTIDAGAVSALRAGKSLLPAGVIRVDGQFARGDAVVVRGPDTHEIGRGLVA
                     YDADDAEKIKGRSSPDVMTILGISGRAEMIHRDDLVVGPSGAFSAQ"
     gene            complement(6844..7893)
                     /gene="obgE"
                     /locus_tag="VN968_07070"
     CDS             complement(6844..7893)
                     /gene="obgE"
                     /locus_tag="VN968_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HXL29035.1"
                     /translation="MKFLDEAKVYIRSGDGGNGCVAFRREKYIEFGGPSGGNGGRGGD
                     VVIEVVDGLNTLIDYRYQQHFKAQKGTNGMGKDRHGANGKPIVLKVPVGTQIFDEDRE
                     TLIHDFTTLGEKFVLAEGGNGGFGNAHFKSSTNRAPRNANPGLEGEERWIWLRLKLIA
                     DAGLVGLPNAGKSTFLSVVSAAKPKIADYPFTTLHPQLGVVDVDGREFVLADIPGLIE
                     GAHEGAGLGDRFLGHVERCRVLLHLVDATCEHAGKAYKTVRTELDAYEGQLTDKIEIV
                     ALNKIDAVTPEQLKAQKDRLKRAAKKTPLLMSGVTGAGVKDALRALVELIGEAPVSVK
                     AKGAAQAEPWAPQAT"
BASE COUNT         1379 a         2525 c         2530 g         1529 t
ORIGIN      
        1 gatgtcgttg gccatgatgc cggccttgga ggcgggcgta tcatcgatcg gcgagacgac
       61 cttgatcagc ccgtcttcca tcgtgacttc gatgccgagc ccgccgaatt ccccgcgggt
      121 ctgcacctgc atgtcgcgga aactcttggc gtccatgtaa ctggaatgcg gatcgaggcc
      181 ggtcagcatc ccgctgatgg cggattcgac gagcttgctg tcgtcgggct tctcgacata
      241 gtcgctgcgc acccgctcga aaacatcgcc gaacaaatta agctggcgat aggtatccga
      301 cgtcgcggcg cgtgcactgg accccatcat cacggcgcgt ggctgcgtca cgaagagcgt
      361 caatgccgcg ccggtggcgg cgctaaggag aattagagaa gtcttgcgca tcatccgcga
      421 accttttcgc cttcatttgc ggcccaccat ggacctggat caattggagt gccgtcctta
      481 cggaactcga catagagcac gggctgactc gcgttggcgg cgagaatgga tgccacctgg
      541 gattttgtcc ccatggtggc gaccggctcc cctgtaagta caaactggcc gatgttaacc
      601 gaaatgcgct ccatcccggc gatcaacaca tgatacccgc ccccggcatt caggatcaag
      661 agttgtccgt agctgcgaaa cggcccggcg taaacaaccc aaccatcaca cggtgttgtg
      721 acctgggctc cggcccgagt tgacaaagaa atgccttttt caacgcctcc ggaaccatcg
      781 gaaccgccaa actcgcgaat cttgacgcca ttaaccggta aagcgaataa acccttggcg
      841 gaagcaaaag caatcgccgg gctcagccgg gcggagtcct tcagccctcc gagatttggt
      901 ttgccattcg gagcaacggg ggcgccccgc aggctggcgc tcgcggccgc tcgcgcggca
      961 ctcttcaaat cctgctccat cttggcgatc aggccctcga ggccgtcggc ctgctgggac
     1021 agcgcgatag cgcgcgcgct ttccgcctcc atgtcttttt cgatcgaatt ctgttttcgc
     1081 tgccgctcat cgacgagcgc ggccagccgg gtttggtcat ccctgagttt gtcgcgatca
     1141 accgcaagct ggtcgcgctc gctggcgatg gacttgcgaa ccgagaccag ttcgccgagg
     1201 tcggtggcca gtctgtccgc acgtccgcgc aattcgggca caacggctcc gagcagcatg
     1261 gccgtgcgaa gcgactgcag cgcgtcttcc ggccttacca gcagggccgg cggcatccgc
     1321 cgtcccgcgc gctgcagcgt agccagcacc tcgacaattt cggaacggcg cgaaccaagc
     1381 gaaccgcgaa tctgctgctc gcgagagtcg agcgggcgca gccgggtttc ggcgtcgcct
     1441 atcctggcct cgacgctgcg cacctgtccg gcgatgtcga tcagccgctg attgagcttg
     1501 ctgcgatcct gaccgatcgc ggcgagatcg gcctggagct tcagctgcaa ctccgccgcg
     1561 ctcttttgct gcgcgcgggc ggcttcgagt tcctgctcgc gctgtttgat cgcgtcaggt
     1621 gacgcagccg tcgtctgctg tgtcgatgcc gcgtcctgcg ccgcaggcaa gacctgggct
     1681 ggtgtcggca aggcttgggc cgccgccggc aaggcttggg ctgccgacgg ccaggcttgg
     1741 gctgccgccg gcaagacctg ggccgccgcc ggcaacaggc tcatgccggc aaagcctgcg
     1801 gacaacaagg caatcgaaag cggaaggcgg actgaggaca gctgaccgcg gcgagcggtg
     1861 tcagggtatg aacgaatgta gggcggtgcc tgcatttagt ccgaatcagt gtttttcaat
     1921 gcttccgcgg ggcgcggctc aaacgcgatg ataggggtgg cccgccaata tggtggcggc
     1981 ccgataaacc tgttccagaa gcatgacgcg aaccatttgg tgcggccagg tggcggagcc
     2041 gaacgcaatg ttcagttttg ccttgcgccg caaatcgggc gaaagtccgt ccgcgccgcc
     2101 aatcacgaaa atagtattgg cgacctgttc atcccgccag cggccaagat gccgggcaaa
     2161 agcggcgctg tcgatgctct cgccgcgctc gtcgagcgcc accagcaccg atttcgtcgg
     2221 aattgccgcc gaaatagcgg cggcttcgtc ggtcatccgg gttgcggcgt cgcgggcgcg
     2281 gctttcggga atttcctgga tctcaaggcc gcgaaaaccg agtttgcggc cgatatcgtc
     2341 gaatcgctcg cgatagcgct cggccaactc ccgctccggg ccctgcttca gccggccgat
     2401 tgagatcacg acgagccgca tcagcgcatc ttccggcaaa gcatgccttt ggagttgatc
     2461 cgaaggtgca atccggtttg ccgcagaaaa tgctccaaat caataattcg cgcgcgttcc
     2521 gcccgcaaaa ccgcaaatcc gttccccgga tcaagtccaa ggacatgcgg cggcacgcgc
     2581 agcccggcgc cagcatgcgc ctaagcctat cccatcggcc cgatcgtcag accggcagat
     2641 cagatggcct tgagctcaga tagccctgaa ctcagatagc cttggccgcc gtcgggctct
     2701 gggtccacaa tctttccaga ttgtagaact cacgcacctc cggcctgaat acgtgcacga
     2761 tcacgtcccc ggaatcgatc agcacccagt cgcagttggg taagccctcg atgtggatac
     2821 ttttgatccc ggtttccttg agacctttgg taacattttc cgcgatcgcg ccgacgtgcc
     2881 ggttggcccg gcccgaagtg acgatcatgt agtcggaaaa cgcagattta ccgcgaaggt
     2941 cgatggtgac cgtttcttcc gccttcatat catcgaggcg ggagaggatc atattcaggg
     3001 tcttgtcggc gtcgggttgc gccttcaagg ccgcggcttg tgtcgatgtt ttacgcgccg
     3061 ttttgggagc cttatcagtg gccttgggta aaacggactt ggacaataca gatgtggcca
     3121 gggaccattc ctttcactgt accgcggacg ccgaatcctg gcgccgctga ctatactacg
     3181 catgtggggt taacggtttc aacattccag tgagccccaa ttctcacttc acgcccgcct
     3241 gtcccctctg tgttctccag ctgccgtccg ggttccgcag gccggtcgat gacaggttca
     3301 atttcatgcc ggtcagaaag acccaggccg gcgcacgctg gtcggccagt cggcctgcct
     3361 ggttctcggg cagccgataa tgcgcgagcg cctgggctgc gggcgcggcc agcgcgcgaa
     3421 agctttgcgg cggccggtcg atcacggcga tcggcgcctc ggctgcgatg cgccgccaat
     3481 tctgccagcg atgaaattgc gcgagattgt cggcgcccat gatccagaca aagcgcaggc
     3541 cggaagcgcg gcggcgcaaa taagtgatcg tgtcgaccgt gtatctggtt cggatgacgg
     3601 attcgagaca gctcacgtcg atgcgcggat catttgccat tttgcgcgcg gcaatcatgc
     3661 gttcttcgag atcgtgcaag gcccgctggt ccttgagcgg attgcccggc gtcaccagcc
     3721 accacacgcg gtcgagcttc agccgcttga tcgcgaacag gctgatggcg cgatgcgcga
     3781 catggggcgg attgaacgag ccgccgagca ggccgatgcg catgccgttg gtatagagcg
     3841 ggatggcctg ggagatggat ggcaacgcga ttggagattg attcaacgga gccaccgcaa
     3901 cgtccgcaac gttgttttcc tgtttgaggg gcgagttttc gcttgaaaac gctgtgacgt
     3961 tcacggccgg gtctgccccg tgccatggac gcggtatttg aaactggtga gctgctcggc
     4021 gccgaccggg ccgcgggcgt gaaatttgcc ggtggcgatg ccgatttctg cgccgaagcc
     4081 gaattcgccg ccgtcggcga actgggtcga cgcgttatgc agcacgatcg ccgaatcgac
     4141 ctcgttgagg aatttgcggg cggcgcctgc atcttccgtc acgatcgcat cggtgtgatg
     4201 cgaaccgtgg ttgtgaatat gcgcgatcgc ctcgtcgacg ccgtcgacca ccttcgcggt
     4261 gatgatggcg tcctcatatt cggtatccca gtcttcgtcg gaagccggtt gtacgcgcgg
     4321 atcggcgtgc tgcacggcgt cgtcgccgcg cacctcgcag ccggcatcga tcagcatcgc
     4381 aaccagcggc ttgagattgg tcgatgcgcc cttacggtcg accagcaagg tttcggcggc
     4441 gccgcagacg ccgggacggc gcatcttggc gttcagcacg atcgacttcg ccatttcgag
     4501 gttggcggcg tgatcgacat agacatggtt gacgccttcg agatgcgcaa acaccggcac
     4561 gcgcgcttcg gcctcgacgc gggcgaccag gctcttgccg ccgcgcggca cgatcacgtc
     4621 gacgccgccg ttcagcccgg ccagcaagag gccgaccgcg gcgcgatcgc gggtcggcac
     4681 cagcgtgatc gaagcctccg gcaggccggc ttcgcgcaag ccctgcacca gacaatcatg
     4741 gatcgcccgg caggaacgaa aactgtcgga gccgccgcgc aggatcaccg cgttgccgga
     4801 tttcaggcac agcacaccgg catcggcggc gacgttgggg cggctttcga agatcaccgc
     4861 gacgacgccg agcggcaccc gcacgcgctc gatgatcatg ccgttcgggc gctgccagct
     4921 ctcggttacg accccgatgg gatcggcgat gtcgcggacg gtcgcgacgc cgtcggccat
     4981 cgcatcgatg cgcgcctgtg tcagcgtcag ccgatcgacg aaggcgctgc tggcgccgcc
     5041 ggcgcgaact tccgcgacat cttcggcgtt ggcgccaagg atcgcggagg cattggcgcg
     5101 aatggcgcgc tcgatcgcat caagcgcgtg gtttttctgc tcgggggagg cgagcgccag
     5161 cacccgcgcg gcggcgcgcg cgtggatagc aaggccggtc atcaggaccg gcaaatcggc
     5221 attgccgtcg attgccttca gcggcgcgct catcttggtt cgagtcccag ctttcagtta
     5281 agaccatatc ctagcacgga aatccgcttt ttacgaggtg cgggccgggc gttgctgggg
     5341 cgggcggggc ggtgccagct cttccgggtt ggcgtgcggc cggcccggtc gccactcggc
     5401 cattggccta ctgtgcggaa aaggcgccgc tcgggccgac caccagatcg tcgcgatgga
     5461 tcatctcggc ccgtcccgag ataccgagga tggtcatcac atccggcgac gagcggccct
     5521 tgatcttctc ggcgtcgtcg gcgtcatagg caacgaggcc gcggccgatc tcgtgggtgt
     5581 ccgggccgcg cactaccacg gcatcgccgc gggcaaactg cccgtcgacc cggatcacgc
     5641 cggccggcaa caggctcttg ccggcgcgca aggcgctcac cgcgccggca tcgatggtca
     5701 gcgtccccct tggctccaat gagcctgcaa tccatcgctt ccgcgcggtc acgggattgg
     5761 ccggcgtcag aaaccaggtg cagcgcccgc catcagcaat ggcctgcagc gggtgctcga
     5821 tcttgccgga cgcgatcagc atatgcgtac ccgacgtggt ggcaatcttc gccgcctcga
     5881 tcttggtgcg catgccgcca cgcgacagct cggattccgc ggcacccgcc atcgcctcga
     5941 tttgcgatgt gacggtttcg acaatcgcaa tcagccttgc gtttggatta ccggcgggcg
     6001 gtgcatcgta gaggccgtcg atatcggaga gtaggatcag aaggtccgcg ctcgtcatgg
     6061 tggcgacccg ggcggcgagg cgatcattgt cgccgtagcg gatttcagtg gtggcgacgg
     6121 tgtcgttttc gttgatcacc ggcaccgcgc gccattccaa cagtttggcg atggtcgagc
     6181 gggcgttgag ataacggcgg cgttcctcgg tgtcctgcag cgtcaccagg atctgcccgg
     6241 cgccgatgcc gtgatgccct agcacctccg accagattcg cgccagcgcg atctgtccca
     6301 ccgcggcggc gccttggctc tcctccagct tcagcgtgcc gcgcggcagt tttaggcggc
     6361 tgcggccgag cgcgatcgaa cccgacgaga cgatcaggag ttcgcgcccc tcgccatgca
     6421 gttttgccag gtcggcggct agcgccgcca gccatgccgc ccgcacttcg ccggcatccg
     6481 agtcgatcag cagcgacgag ccgaccttga cgacgatgcg gcggaagttt ttcagctctg
     6541 ggcgggccat ggaatgagac tttgcggctg ggcttcgaag agtgcgcgga atgctaaggg
     6601 attgcagcga cggaagcgcg ctccctctcc cacccaattc gggtttaccc gagttgggca
     6661 tatcaaattg tcgaagccgg atatatccga cttcgagcgg gagagggttg gggtgagggg
     6721 gtacggtcca tcgtcagtcc ttaacccctc acccggatcg catatgatgc gatcccacct
     6781 ctcccgatgg gagaggtggg actgaactcg cggcaggctc cgttgcgatc catgtttcac
     6841 gcgctaggtc gcttgcggcg cccatggctc ggcctgcgcc gcgcccttgg ccttcaccga
     6901 caccggggct tcgccgatca actcgaccag cgcccgcagc gcgtccttca cgccggcacc
     6961 cgtgacgccc gacatcaaca gcggggtctt cttggcggcg cgcttcagcc ggtccttctg
     7021 cgcctttaat tgttccggcg tcaccgcgtc gatcttgttc agcgcgacga tctcgatctt
     7081 gtcggtgagt tgtccctcat aggcgtcaag ctcggtgcgc accgtcttgt aggccttgcc
     7141 ggcgtgctcg caggtagcgt cgaccagatg cagcaagaca cggcagcgct cgacatggcc
     7201 caaaaaccgg tcgccgaggc cggcgccttc atgcgcgcct tcgatcagcc ccggaatgtc
     7261 cgccagcacg aattcgcggc catcgacatc gaccacgccg agctgcggat gcagcgtggt
     7321 gaagggatag tcggcgatct tcggcttggc agcgctgacg acggagagga aggtcgactt
     7381 gccggcgttg ggcaggccga ccaggccggc atcggcgatc aacttcagcc gcagccaaat
     7441 ccagcgctcc tcgccttcca ggccggggtt ggcgttgcgc ggcgcgcggt tggtggacga
     7501 tttgaaatgt gcattgccga atccgccatt gccgccttcg gcgagcacga atttctcgcc
     7561 gagcgtggtg aaatcgtgga tcagtgtttc gcgatcctcg tcgaaaatct gggtgccgac
     7621 cggcaccttg agcacgatcg gcttgccgtt ggcgccatgg cggtccttgc ccatgccgtt
     7681 ggtgcccttc tgcgccttga agtgttgctg atagcggtag tcgatcaggg tgttcaatcc
     7741 atcgaccacc tcgatgacga cgtcgccgcc gcggccgcca ttgccgccgc tcggtccgcc
     7801 gaactcgatg tacttctcgc ggcgaaacgc cacgcagccg ttgccgccgt cgccggagcg
     7861 gatatagact ttcgcttcat caaggaattt catggcctat aggtagggca ggggcgccga
     7921 tgcggcaacc tttagatgcg gtaaaattga cgaaaagcct taa
//
LOCUS       DATNOI010000184         5730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2011091,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000184 DATNOI010000000
VERSION     DATNOI010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011091"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VN968_07075"
     CDS             <1..602
                     /locus_tag="VN968_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXL29036.1"
                     /translation="AVERRAGRGSDADVDAARSGLTRAQVRLVQQKAELRRIEADASL
                     PTQAEGQLNIARSELLATQAAIEKMTIRAPIDGTVLQMNAKPGELASPSATQALVLFG
                     NVSALRVRAEVDERDIEKIKAGQSVLVRPAAFRDREIAGTVAFIAPLVESGRYNALGQ
                     RNMTDVDVVEVLVNLGEPGPLAVGMKVDVYFRQDRASRN"
     gene            639..785
                     /locus_tag="VN968_07080"
     CDS             639..785
                     /locus_tag="VN968_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29037.1"
                     /translation="MPFAGQNGRAPKIPWIIWFGRDSDESETAIAATASRYKMVMQNQ
                     KKSR"
     gene            complement(987..1130)
                     /locus_tag="VN968_07085"
     CDS             complement(987..1130)
                     /locus_tag="VN968_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29038.1"
                     /translation="MRTKKIRGEDSFAGSISIAELEEGPEGIADVIRCEEAVSGINMA
                     QRG"
     gene            1078..2049
                     /locus_tag="VN968_07090"
     CDS             1078..2049
                     /locus_tag="VN968_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HXL29039.1"
                     /translation="MEIEPAKLSSPRIFLVRMLVFLVLCALVMVVLYKQIVTAFFANP
                     GLNALIGAVLAIGIILSFRQVIRLYPEVAWVNNFRIADPGLAVERLRPTLLAPMAAIL
                     GGERTGRMTISQQTMRHLLDSIATRLDEARDISRYMTGLLVFLGLLGTFWGLIETVGS
                     VGKVIDGLKVGGDAGALFDTLKEGLAAPLGGMGISFSSSLFGLAGSLILGFLDLQSSQ
                     AQNRFYTDFEDWLASTVRGYSSESGSASGGELQAAVTQLRATLDEGGGNRGTTAAMAN
                     LAEAIQGLVAHMRNEQQMIREWADGQGEQNREIKKLLERIARQPEKN"
     gene            2327..3358
                     /locus_tag="VN968_07095"
     CDS             2327..3358
                     /locus_tag="VN968_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HXL29040.1"
                     /translation="MALARTRRSETGFNYWPGFVDALSTLVLSIVFLLSVFLVVQFFL
                     SQEVTGKDKALEQLNAKIAQLNELLSLEKLGKLNLDDQISQLRAGLASAEAERNRVKG
                     LYEGLAGAGNDAAGRAAELNKALDSEKVISARALAQIEVLTQQITALRRQLAALEEAL
                     DASEKRDKESQGRIADLGQRLNVALAQRVQELSRYRSEFFGRLRAILGNRPDIRIVGD
                     RFVFQSEVFFDTGQALLLPEGRAELDKLATALIDLDKQIPAEIAWVLRVDGHTDVRPI
                     LNSPIFRSNWELSSARAISVVQYLISLGVPAQRLVAAGFAEFQPLDTAANEDAYKRNR
                     RIELKLTER"
     gene            3484..3933
                     /locus_tag="VN968_07100"
     CDS             3484..3933
                     /locus_tag="VN968_07100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL29041.1"
                     /translation="MSATFHLRPYCADDEDAAIALWQQTWQQAYPDIDFAARVTWWRE
                     RWRRDLVPNAAIMVAEEAGALIGFVTIDRKGYLDQLVVSPDRWGSELGNALVDEAKRL
                     SPDGIMLLVNTDNARAIRFYARNGFVRAGADVNPTSGRPVLRMEWKG"
     gene            complement(3979..4179)
                     /locus_tag="VN968_07105"
     CDS             complement(3979..4179)
                     /locus_tag="VN968_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29042.1"
                     /translation="MPFRRGGFALTPPSVLIFVISLALAVLALLVHYGHVSVPIIGVS
                     HVFDVLAIAYVVLMVGVLFRGI"
     gene            complement(4386..>5730)
                     /locus_tag="VN968_07110"
     CDS             complement(4386..>5730)
                     /locus_tag="VN968_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL29043.1"
                     /translation="GASVSIALRNLMLFIGATVMMVITSPRLSGFVLLAIPAIVIPLV
                     AFGRWVRRLSRNAQDTLADATAYASELVGAIRTVQAYTSERLANARFGGEVEQAYEAA
                     RSSTRARAVLTAIIIFVVFTSVVAILWVGSHDVLTGSISPGRLGQFVLYAAFAAAGLG
                     QLSEVWGEVSAASGASERLFEILRVKSAISAPALPRAMPVPARGDVGFEKVSFAYPTR
                     LDALAVNGVSLTVRAGEKVAIVGPSGAGKSTLFHLLLRFYDPAAGVISFDGVPIREAD
                     PRELRSRIALVPQDSVVFAATARENIRFGRPEASDAEVERASDLAHATEFIRRLPGGF
                     ESQLGERGVTLSGGQRQRIAIARAILRDAPLLLLDEATSSLDAESETLVQTALEELMR
                     HRTTLVIAHRLATVLSCDRIMVMDQGRIVEQGTHASLVAANGLYARLARLQFEGV"
BASE COUNT         1148 a         1794 c         1787 g         1001 t
ORIGIN      
        1 cggccgtcga aaggcgggcc ggccgaggtt cggatgcaga tgtggacgcg gcccgttcgg
       61 gtttgacgcg tgcgcaagtt cgcctggtcc agcaaaaagc cgagcttcgc aggatcgagg
      121 ccgacgcgtc gctgccaacg caggccgaag gccaactcaa catcgcgcgc agcgaactgt
      181 tggcgaccca ggcggccatt gaaaagatga cgatacgtgc gccgattgac ggcaccgtct
      241 tgcaaatgaa tgccaagccg ggggaactgg cgtctccatc ggcgacgcag gcgctcgttc
      301 tgttcggcaa cgtttcggca ttgcgcgtgc gtgccgaggt ggacgaacgt gacattgaaa
      361 aaataaaggc cggacaatcg gtgctggtgc gtcccgccgc gtttcgcgac cgcgaaatcg
      421 cgggaacggt cgccttcatt gctccgctgg tggagtccgg acgctacaat gcgcttggcc
      481 agcgcaatat gactgacgtc gacgtggtgg aagtgctggt caacctgggc gagcccggtc
      541 cgctcgcggt cggcatgaag gtggacgtgt atttccgcca ggatcgcgcc tcgcgcaatt
      601 gagctccgtc gtgcttgcac gctactatgg ctaacaacat gccgtttgcc ggccaaaatg
      661 gaagggctcc gaaaattccc tggatcattt ggttcggccg cgacagcgac gaaagcgaaa
      721 ccgcgatcgc ggccaccgct tcgagataca aaatggtcat gcaaaatcaa aaaaaatcgc
      781 gctagagcct tgtccgtttc ggtagaatcg aaacggacaa ggctctagat tctcgatttg
      841 acgcgttttc ttgacgcgaa ccggactcca cttcgcttga aaacgcgctg gacaccaacc
      901 attcgtgaag tacgctctca cccaccccgg tttcaaccag aggttccgca tgtcacctga
      961 aatcaggacg tatccgagct tttttctcag ccccgctgtg ccatattgat gcccgaaacc
     1021 gcttcctcgc aacggatgac atcggcaatg ccttcaggtc cctcttccag ctccgcgatc
     1081 gagatcgaac cggcaaaact gtcctcgccg cggattttct tggtccgcat gctggtcttt
     1141 ctggtgctgt gcgcgctggt catggtcgtg ctctacaagc agatcgtcac ggccttcttc
     1201 gccaatcccg ggctcaacgc gctgatcggc gcggtgctgg cgatcggcat cattttgtcg
     1261 tttcgtcagg tgatcaggct ctatcccgag gtcgcctggg tcaacaattt ccgtatcgcc
     1321 gatcccggcc tcgcggtcga acggctacgc ccgacgctgc tggcgccgat ggcggcgatt
     1381 ctgggcggcg agcgcaccgg ccgcatgacg atctcgcagc agaccatgcg gcatctgttg
     1441 gattcgatcg ccacgcggct cgacgaggcc cgcgacattt cccgctatat gaccggcctc
     1501 ctggtgttcc tcgggctgct cggcacgttc tggggcctga tcgaaaccgt cggctcggtc
     1561 ggcaaggtga tcgacgggtt gaaggtcggc ggcgacgccg gcgccctgtt cgacaccctc
     1621 aaggaaggtc tggccgcgcc gctcggcggc atgggcattt cgttctcatc ttcgctgttc
     1681 ggcctcgccg gttcgctgat tctcggcttt ctcgatctgc aatcgagcca ggcgcagaac
     1741 cggttctata ccgacttcga agactggctg gcctccacgg tgcgcggata ttccagcgag
     1801 agcggctcgg cttcgggagg cgaactccag gccgccgtca cgcagttgcg ggcgacgctg
     1861 gacgaaggcg gcggcaaccg cggcaccacc gcggcgatgg ccaacctcgc cgaagccatt
     1921 cagggtctgg tcgcccatat gcggaacgaa cagcagatga tccgcgaatg ggccgacggc
     1981 cagggcgagc aaaaccgcga gatcaagaaa ttgctggagc ggatcgcgcg ccagcccgag
     2041 aagaactaga cgaattagga agggtgcaaa agttgaaaca gcggaaacgc actccctctc
     2101 ccacccaact cgggtttacc cgagttgggc atccaaatct aacgaagtcg gatatatccg
     2161 acttcgatgg gagagggttg gggtgagggg atacgaccta tcgttaggac tgcaacccct
     2221 cactcggatc gcgagagcga tccgacctct ccccatggga gaggtggacc tgaactcgag
     2281 atagattctg actcaccggt cagattctac agcggagaac tctcacatgg ccctcgcccg
     2341 tacccgccgc agcgaaaccg gtttcaacta ctggccggga ttcgtcgatg cgctgtcgac
     2401 gctggtgctt tcgatcgtgt tcctgctgtc ggtgttcctg gtggtgcagt tcttcctgtc
     2461 gcaggaagtc accggcaagg acaaggcact cgagcagctc aacgccaaga tcgcccagct
     2521 gaacgagctg ctgtcgctgg aaaaactcgg caagctcaat ctcgacgacc agatctcgca
     2581 gctgcgggcg gggctggcat cggcggaagc cgaacgcaat cgcgtcaagg gtctctatga
     2641 aggcctcgcc ggcgccggca acgacgccgc cggccgcgcc gccgaactca acaaggcact
     2701 ggactccgaa aaggtgatat cggcgcgcgc gctggcgcag atcgaggtac tgacccagca
     2761 gatcaccgcg ctgcgccgcc agctggcggc actcgaagaa gccctcgacg cctccgaaaa
     2821 gcgcgacaag gaatcgcaag gccgcatcgc cgacctcggc caacgattga acgtggcact
     2881 ggcgcagcgg gtgcaggaat tgtcgcgcta ccgttcggaa ttcttcggaa ggctgcgcgc
     2941 catcctcggc aaccggcccg acatccgcat cgtcggcgac cgttttgtct ttcagtcgga
     3001 agtatttttt gacaccggac aggccttgct gttgcccgag ggccgcgccg agctcgacaa
     3061 gctggcaacc gcgctgattg atttggataa gcagatcccg gccgaaatcg cctgggtgct
     3121 gcgggtcgac ggccataccg acgtgcggcc gatcctcaac agtccgatct tcaggtcgaa
     3181 ctgggagctg tcgtcggccc gcgcgatctc ggtcgtgcag tatctgatct cgctcggcgt
     3241 gccggcgcaa cggctggtcg cggccgggtt tgccgaattc cagccgctcg acaccgccgc
     3301 caacgaggac gcctacaagc gcaaccgccg catcgagttg aagctgacgg agcgctgagg
     3361 gcacgatgcc aaaaggaccc cgcagatcgt cgttgccggg cttgacccgg caatccatca
     3421 aaagagactt ttgccaagca gatcgatgcc cggatcaggt ccgggcatga cggcgttgcc
     3481 tggatgagcg ccacctttca ccttcgcccc tattgcgccg acgacgagga cgccgcgatc
     3541 gcgttatggc agcaaacctg gcagcaggcc tatccggaca tcgatttcgc cgcgcgcgta
     3601 acctggtggc gcgagcgctg gcgaagagat ctggtgccga atgcggcgat catggtcgcg
     3661 gaagaggccg gcgcgctgat cggatttgtg accatcgatc gcaaaggcta tctcgaccag
     3721 ctggtcgtca gtcccgatcg ctggggttcg gaactgggta atgcgctggt cgatgaggcc
     3781 aaacgcttgt cgcccgatgg catcatgctg ctggtcaaca ccgataatgc ccgcgccatc
     3841 cgcttctatg cgcgcaacgg gtttgttcgc gccggcgcgg acgtcaatcc gacctcggga
     3901 cgccccgtac tgcggatgga gtggaagggg tagaacgccg tttcgccact tccgcgcgaa
     3961 agcggctgga ttggggttct aaatcccgcg aaacagcacg cccaccatca acacgacgta
     4021 ggcgatggcc agaacatcaa acacgtgcga cacgccgatg atcggcaccg aaacatgccc
     4081 atagtgtacc agcaaggcga gaaccgccaa tgccagcgaa atcacgaaga tcaggaccga
     4141 gggaggcgtg agcgcaaaac cgccgcgacg aaaaggcatg cttatctcca ttttgcttca
     4201 tgcccggcaa atacaaggct gcagctgcgg acaaactgcc aaggccgaca agcgtttcaa
     4261 ttgcggaaca aaattctcga tcatgatccg acgagagtcg gatcatcatc tcattcctgg
     4321 tttgagcatg gtcttttcga aagcctaatc cccacgggat cgcgccaagg ggaagtagag
     4381 agggcttaca ctccctcgaa ctgcaatctc gccagcctag cgtagagacc gttggcggcg
     4441 accagcgagg cgtgggtgcc ctgctcgacg atgcggccct gatccatcac catgatgcgg
     4501 tcgcaggaca atacggtggc gaggcgatgg gcgatcacca gggtggtgcg gtggcgcatc
     4561 aattcttcca gtgcggtctg caccagcgtt tcggattccg catcgagcga cgaggtcgct
     4621 tcatcgagca gcaggagcgg cgcgtcgcgc aggatggcgc gcgcgatcgc gatgcgctgg
     4681 cgctggccgc ccgacagcgt cacgccgcgc tcgccaagct gcgattcgaa tccgcccggc
     4741 aggcggcgga tgaattcggt ggcgtgggca aggtccgacg cgcgctcgac ctcggcatca
     4801 gaggcctcgg gccggccgaa gcggatgttt tcccgcgcgg tcgcggcgaa caccaccgaa
     4861 tcctgcggta ccagcgcgat gcgggagcgc agctcgcgcg gatcggcttc cctgatcggc
     4921 acgccgtcga acgaaatcac gcccgcggcg ggatcgtaga accgcagcag caaatgaaac
     4981 agcgtgctct tgccggcgcc cgacgggccg acgatcgcga ccttctcgcc ggctcgtacc
     5041 gtcagcgaaa cgccattcac cgccagcgca tccagccggg tcggataggc aaagctgact
     5101 ttctcaaacc cgacgtcgcc gcgcgccggt accggcatgg cgcgcggcaa ggccggtgcg
     5161 gagatcgcgg atttgacgcg aaggatttcg aacaatcgct ccgatgcgcc ggaggcggcc
     5221 gaaacttcgc cccagacctc gctgagctgg ccgagccccg cggccgcgaa cgccgcatac
     5281 agcacgaact ggccgagccg gcccggactg atggagccgg tcagcacatc atgcgatccg
     5341 acccagagga tcgccacgac actggtaaag acgacgaaga tgataattgc cgtaagaacg
     5401 gcgcgggcgc gggtcgaact gcgcgccgct tcataggcct gttcgacttc gccgccgaag
     5461 cgcgcattgg ccagccgctc gctggtatag gcctgcacgg tgcggatagc gccgaccagt
     5521 tcggaggcat aggcggtggc atccgccagc gtatcctggg cgttgcgcga caaccggcgc
     5581 acccagcgcc cgaacgccac cagcggaatc acgatcgccg gtatcgccag cagcacgaag
     5641 cccgaaagcc gcgggctggt gatcaccatc atcacggtgg cgccgatgaa cagcatgagg
     5701 ttgcgcagcg caatcgatac cgaggcgccg
//
LOCUS       DATNOI010000185         8027 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2584572,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000185 DATNOI010000000
VERSION     DATNOI010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8027
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2584572"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VN968_07115"
     CDS             complement(<1..764)
                     /locus_tag="VN968_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL29044.1"
                     /translation="MRKVFCHLISAGVLVILPVATANAADPAAREPYGIGLEGFAYPY
                     PVSMLPLTNDGEQVRMAYMDVAPAQPNGRTVVLLHGRNFPSSYWAPVIKTLTDAGYRV
                     VVPDQIGFGKSSKPSADLHFDTLARNTNALLDHLQIAKAEFVAHSLGGMLGVRIARAY
                     PDRVAHLLLAAPIGLEDYRLYVPPTPTEKILENEDRLTADGYRKQLQTNYSLKLPPDQ
                     VTPFIDARFNIKGSSEYPRWLRAFVSSAQMIYREPVV"
     gene            complement(961..1656)
                     /locus_tag="VN968_07120"
     CDS             complement(961..1656)
                     /locus_tag="VN968_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXL29045.1"
                     /translation="MKAGPDIAMVAALVGDPARSNMLTALMTGRALTASELAHQAGVT
                     PQTASSHLSKLEAGGLIEPEKQGRHRYYRLTGPDVAGVLEGLAGLAARAGHMRVRTGP
                     KDPALRRARICYDHLAGDLGVQMLDSLKKQRLVRQSKQAIELTAEGKRFMENSLQISA
                     EALAHPRRPVCKACLDWSERRHHLAGTLGAAMMTRFAELKWAARDPTPGSRVVNFTRN
                     GEKHFAELFGKSE"
     gene            1732..2040
                     /locus_tag="VN968_07125"
     CDS             1732..2040
                     /locus_tag="VN968_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HXL29046.1"
                     /translation="MAVTVFIRYQIDPFKRAMFEEYARRWLTIIPKCGGDLVGYWVPH
                     EGTNNIAFALISFESLAAYESYRARLRADQEGMANFSFAEDNKFIVAEERTFLRKVTL
                     "
     gene            2085..2435
                     /locus_tag="VN968_07130"
     CDS             2085..2435
                     /locus_tag="VN968_07130"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HXL29047.1"
                     /translation="MIAVIFEVWPKPEHRGEYLDLAAELRPILETIDGFISIERFESL
                     TEKGKILSLSFFRDEEAVAAWRNIPQHRKTQAKGRARIFDNYRLRIAGVVRDYGMTDR
                     TQAPNDSRATHDAH"
     gene            2498..3313
                     /locus_tag="VN968_07135"
     CDS             2498..3313
                     /locus_tag="VN968_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HXL29048.1"
                     /translation="MPVTTAEKRATFRKMHESGCFAIPNPYDVGSALALQHLGFKALA
                     STSAGFAWTIGKADNRVTLDEVCTHLTALCATVDLPVNADFEGGFAHEAEKVGANVAR
                     AVKTGVAGLSIEDSTGEAAKPLYERGHAIERIEAARAAIEADNSGVLLTGRCEAFLVG
                     QADLDLVIDRLKAYSDAGADCLYAPGIKTREQISAVVKAVHPKPVNLLIGASGLSIAE
                     AGDLGVRRISVGGSLARSAWAGFMEAAREIADKGTFTELASGYSGAELNKMFK"
     gene            complement(3380..3829)
                     /locus_tag="VN968_07140"
     CDS             complement(3380..3829)
                     /locus_tag="VN968_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29049.1"
                     /translation="MANPRNPNDPYRSPDDPYRSNPTDDEIRRQAQLDSELQPDPELA
                     EGPASGGKIAMFAVAIALVLGAVFYGLNSSTVNQAGTSSSTAQNTPQSSPPAAPPGMR
                     DVTPRPNTDQGVTTGAAPARPQTPPASAPTEMNRPDHPPANNAAPSK"
     gene            4180..4416
                     /locus_tag="VN968_07145"
     CDS             4180..4416
                     /locus_tag="VN968_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL29050.1"
                     /translation="MRKLFEEEGSSFSSFVLAERLLGARRMLVDRRYANLNIAQIAHE
                     NGFGDFSYFNRVFRRHFGATPSDFREAARREWLP"
     gene            complement(4492..5700)
                     /locus_tag="VN968_07150"
     CDS             complement(4492..5700)
                     /locus_tag="VN968_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXL29051.1"
                     /translation="MRAIPAIILLCTAFNLGYAQAEPSAEIIVQGKALADTAGCASCH
                     TADPAKPFAGGKRIDTPFGGIYSPNLTPDRDTGLGAWSDDDFYRALRHGIAPDGSRYY
                     PAFPYPNFTKLIRDDILAIRAYLATLVPISNRPPQAELRWPLNYRVLMRGWDFLFFRP
                     GIFEPNQQKSPDWNRGGYLVEGAAHCGSCHTPKNMFGADKRGRAYGGGLVQGWFAPRL
                     DSAERSGLKSWSVDDIVEYLQSGRNGKSHAGGLMAEVVVHSTSVMSDADVHAIAVYLK
                     DLPAGKREPAVIPPSPELLADGEKIYKWACIACHEVDGSGAPRIYPPLPDNANLQSAD
                     PSSALRIILDGAQTLTTPRAPNGGSMPAYAEKLSDQAIADVTNYIRNAWGNAAPLVTP
                     AQVAKARREK"
     gene            5980..>8027
                     /locus_tag="VN968_07155"
     CDS             5980..>8027
                     /locus_tag="VN968_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXL29052.1"
                     /translation="MGINQGPISLDQKYTQGSGHVFLTGIQALVRLPMAQIRRDRAAG
                     LNTAGFVTGYRGSPLGGYDQQLFAARKHLEQYNIKFQPGVNEDLAATAIWGSQQLNLS
                     PGARYDGAVGIWYGKGPGVDRCGDVFRHGNAAGSAKNGGVLCLAGDDHGAKSSTVPHQ
                     SDHAFISALMPYLYPSSIHEMIEMGLLGIAMSRYSGCWVGMKVITETVETTAEVDLTD
                     EMTPFIIPTDFEMPPGGLNLRWPDDRYEQDRRLQDYKGFAAIAFARANKVNRVTMDSP
                     NARFGIMASGKSYEDIRQALRELGVTAEVAAKIGLRLYKIGMPWPLEPQGVREFAVGL
                     EEIFIVEERREIVENQVKQELFNWRDDVRPRIVGKMDEHDKRFLTFAAELSVASLASS
                     LTERLLRLNLNPEIAEMLRAKADWFNGREATQMQAVAPISRTPYFCSGCPHNTSTKVP
                     EGSRAFAGIGCHFMALWMDRSTETYTHMGGEGVPWVGVAPFTDEKHVFANLGDGTYFH
                     SGSLAIRQAVSSGANITYKILYNDATAMTGGQHVDGELSPQQITFQLHSEGIRDIYLV
                     SENPDAYPASDIAPGTRTAHRDELDAVMKTLREVKGATAIVFVQTCAAEKRRRRKRGT
                     LEDPARRVIINPAVCEGCGDCSVQSNCISVEPLETELGRKRTINQSSCNKDYSCLKGF
                     "
BASE COUNT         1536 a         2467 c         2553 g         1471 t
ORIGIN      
        1 accaccggct cgcgatagat catttgcgcc gagctgacaa aggcgcgcag ccagcgtgga
       61 tattccgaac tgcccttgat gttgaagcgc gcgtcgatga acggcgtgac ctggtcgggc
      121 ggcagtttta gcgaataatt ggtctgaagc tgcttgcggt agccgtcggc cgtcagcctg
      181 tcctcgttct ccagaatctt ttcggtcggc gtcggcggca cgtagagccg gtaatcttcc
      241 aacccgatag gggccgcgag caacagatgt gcgacccgat ccggataggc gcgtgcgatc
      301 cgcacgccga gcatcccgcc cagcgagtgc gcgacgaact cggcctttgc gatctgcaga
      361 tgatcgagca gcgcgttggt attgcgcgcc agcgtgtcga agtgcaggtc ggcggacggc
      421 tttgaggatt tgccaaagcc gatctggtcc ggcaccacca cgcggtagcc ggcgtcggtc
      481 agcgtcttga tcacaggcgc ccagtagctc gacggaaagt tgcggccgtg cagcagcacc
      541 acggtgcggc cgttcggctg cgccggtgcg acgtccatat aggccatccg cacctgctcg
      601 ccgtcattgg tcagcggcag catgctgacg gggtagggat aggcaaaacc ttccagcccg
      661 atgccgtagg gttcgcgcgc ggcggggtcc gcggcgttgg cggttgcgac gggcaagatg
      721 acgaggacgc cagcagagat caaatgacag aaaacttttc gcatcagaca aacccaacct
      781 catgttttgt catgcccggt catagcagtc tgcgtcgtgc agaccgccta cacttgtctg
      841 cgctgccggg catcgacgtc gttactccat agcagcaagt aaggcgtgga tagccgggac
      901 aagcccggtc atgacgggaa gttgaagttg acgtggcagt agcgaaagct ctccgcccgg
      961 ctactcgctt ttcccaaaca gctcggcgaa gtgtttctcg ccattgcggg taaaattgac
     1021 gacgcggctg ccgggggtgg gatcgcgcgc ggcccatttc agctcggcga accgcgtcat
     1081 catggccgca cccagcgtgc ccgcaagatg atggcgtcgc tcgctccagt caaggcatgc
     1141 cttgcatacc ggacggcgcg gatgggccag cgcctcggca gagatctgca ggctgttttc
     1201 catgaagcgc ttgccctcgg cggtgagttc gatcgcctgc ttgctctggc gaaccagccg
     1261 ctgctttttc aggctgtcga gcatctgcac gccgaggtcg cccgccagat gatcgtagca
     1321 aatccgcgcc cggcgcagcg ccggatcctt cggcccggtg cgcacgcgca tgtgaccggc
     1381 gcgcgccgca aggcccgcaa gcccctccag cacgccggcg acgtcggggc cggtgaggcg
     1441 atagtaacgg tggcggccct gcttctcggg ctcgatcaga ccgccggcct cgagcttgga
     1501 aagatgcgaa ctcgcggtct gcggggtgac gccggcctgg tgtgccagtt cgctcgcggt
     1561 cagcgcgcgg ccggtcatca gcgccgtcag catgttggag cgcgcgggat cgccgaccag
     1621 tgctgcgacc atggcgatgt cggggcctgc tttcatagtt cgatgatagc cgaagcatta
     1681 gcgtcgatca agtagctatt tttgggagca atcaaaaccc cggaggttcc cttggccgtc
     1741 accgttttca tccgctacca gatcgatccg ttcaagcgcg cgatgttcga ggagtacgcc
     1801 aggcgttggc tgaccatcat tccgaaatgc ggcggcgatc tagtcggcta ctgggtgccg
     1861 cacgagggca ccaacaatat tgcgttcgcg ctgatttcgt tcgagagtct cgccgcctat
     1921 gagagttatc gcgcaagact tcgcgccgac caggagggca tggccaattt cagtttcgcc
     1981 gaagacaaca aattcatcgt tgcggaggaa cgaacatttc tgcgcaaggt gacgttataa
     2041 aacatcccaa cgtaaaacat cccaacgcac aggaggagac gccaatgatc gccgtgatct
     2101 tcgaggtctg gcccaagccg gagcacagag gagaatatct cgacctcgcg gccgaactgc
     2161 ggccgatcct ggaaaccatc gatggcttca tctcgatcga acggttcgaa agcctgaccg
     2221 agaaaggcaa gatcctgtcg ctgtcgttct tccgcgacga ggaggctgtc gcggcatggc
     2281 gcaacattcc ccagcatcgc aaaacccagg ccaaggggcg tgccaggatt ttcgataatt
     2341 accggctgcg tattgccggt gtcgtgcggg actacggcat gaccgatcgc acgcaggcgc
     2401 caaacgacag ccgggccacg cacgacgcgc actagcgccg cgcgcgcccg gcgattatcg
     2461 tcacgcagct aaccgaacat aaaaacaggg agcgatcatg ccggtcacaa cagcagaaaa
     2521 gcgagcgaca ttccgcaaga tgcacgaaag cggttgtttc gcgattccca atccttatga
     2581 cgtcggcagc gcgctggcgc tgcagcactt gggcttcaag gcgctcgcgt ccaccagcgc
     2641 gggctttgcg tggacgatcg gcaaggccga caatcgcgtc acgctcgacg aggtctgcac
     2701 gcatctcacc gcgctgtgcg cgacggtcga tctgcccgtc aacgccgatt tcgagggcgg
     2761 ctttgcgcat gaggcggaga aggtcggcgc caatgtcgcg cgcgcggtca agaccggcgt
     2821 ggccggtctg tcgatcgagg attccactgg cgaagccgca aaaccgcttt acgagcgcgg
     2881 ccatgcgatc gaacgcatcg aggccgcgcg cgcggcgatc gaggccgaca attccggcgt
     2941 gctcctgacc ggccgttgcg aggcgtttct ggtcgggcag gccgatcttg atctggtgat
     3001 cgaccgactc aaagcctatt cggacgcggg cgccgactgc ctctatgcgc cgggcatcaa
     3061 gacccgggaa cagatttcgg ctgtcgtgaa agccgtgcac ccaaaacccg tcaatcttct
     3121 gatcggtgcg tccggcctct cgattgccga agccggcgat ctcggcgtcc gccgcatcag
     3181 cgtcggtggt tcgctggcgc ggagcgcctg ggccggattc atggaggccg cgcgcgagat
     3241 cgcggacaag ggaacattca ccgaattggc cagcggctat tccggcgccg aattgaacaa
     3301 gatgttcaag taacggccac aacgaaaacg cagcgggcca tctagcctgc tgcgcttccg
     3361 gtctgatgcg cgtccggcgt tacttgctgg gcgctgcgtt gttggccggc ggatgatcgg
     3421 ggcggttcat ctcggtcgga gccgaagccg gcggtgtctg cggacgcgct ggagcggcac
     3481 ccgtcgtcac gccctgatcg gtgttggggc gaggcgtcac gtcacgcatg cccggaggcg
     3541 ccgcgggagg cgaactctgc ggggtattct gggcggtcga agaggaggtg ccagcctgat
     3601 tgaccgttga actattcagg ccgtagaaaa ccgcgcccag caccagggcg atggcgaccg
     3661 cgaacatcgc aattttgccg ccactggcgg gaccctcggc aagctcggga tcgggctgca
     3721 gctcgctgtc gagttgggcc tgacgacgga tctcgtcgtc ggtaggattg gaacgataag
     3781 gatcgtccgg ggaacgatag ggatcgttcg gattacgtgg gttggccatg atgggttcct
     3841 ccgttggccc ataccaaccc ctggcgggag cgggggttcc tgattcgacg cgatattggc
     3901 ggatccaagt tggggaaccg gaggcagaat cacggacggt tcccgccgct atggctgaat
     3961 taataagcga tgccgaacgt ggccgtctga gaaagccgct cgaagagcgc aaaacggctc
     4021 cggacgaagc cggggctttc tcaaagtgtc tcaggagaga aattatgatc aacgcaagag
     4081 atctcgccgc cacatcaatc gacgatggat ggccaagaca gtctcagaac ctatagttca
     4141 cgccgacacg accgatgtcg ccgctaaccc ggtcgttatg tgcggaaatt gttcgaagaa
     4201 gagggctcgt cgttttcctc gttcgtcctc gccgaacgat tgctcggggc ccggcgcatg
     4261 ctggtcgatc ggcgttatgc caacctgaac attgcgcaga tcgcgcatga aaacggtttc
     4321 ggtgatttct cctatttcaa tcgcgttttc cgtcgccact tcggtgccac gccctccgat
     4381 tttcgtgaag ctgccaggcg cgaatggctg ccgtgacgct tcgaggcgcg tcagtcatgt
     4441 ctgggtcgcg tcatttcgtg ccgcgccgcg tccgggacac gtcacaccac gtcacttttc
     4501 gcgccgcgcc tttgcgacct gtgctggcgt gaccaggggc gcggcgttgc cccacgcatt
     4561 cctgatgtag ttcgtgacgt cggctattgc ctgatcggaa agtttctctg cgtaagccgg
     4621 catcgaaccg ccgttgggcg cgcggggcgt cgtcaatgtc tgggcgccgt cgagaatgat
     4681 gcgcagcgcg ctggagggat cggcggactg cagattggca ttgtcgggca atggtggata
     4741 gatccgcggc gcgccgctgc cgtcaacttc gtggcaggcg atgcaggccc atttatatat
     4801 cttctcgccg tccgccagca gctctggcga tggcggaatc accgcaggct cccgcttgcc
     4861 agccggcaga tccttcagat agacggcgat ggcgtggaca tcggcgtcgc tcatgaccga
     4921 ggtcgaatgg accaccactt ccgccatcag cccgccggcg tggctctttc cgttgcggcc
     4981 gctctgcaga tattcgacga tgtcgtcgac gctccaggat ttcagtccgc tgcgctcggc
     5041 actgtcgagg cgcggcgcga accagccctg cacgagaccg ccgccatagg cgcggccacg
     5101 cttgtcggcg ccgaacatgt tcttgggtgt gtggcatgag ccgcaatgcg cggcaccttc
     5161 gaccagatag ccgccgcggt tccagtccgg gctcttttgc tggttgggct cgaatatgcc
     5221 gggccggaag aacagaaaat cccagccgcg catcagcacc cggtaattga gcggccagcg
     5281 cagttccgcc tgcggcggcc ggttgctgat cggcacaagc gtcgcgaggt aggcgcggat
     5341 cgccagaata tcgtcgcgga tcaatttcgt gaaattgggg taggggaacg cggggtaata
     5401 gcgcgagccg tcgggcgcta tcccgtgacg cagggcgcgg taaaagtcgt cgtcgctcca
     5461 tgcgccgagg ccggtatcgc ggtccggcgt caggttcggc gaatagatcc cgccgaacgg
     5521 cgtgtcgatc ctttttccgc cggcaaaggg ctttgcggga tcggcggtgt ggcaactggc
     5581 gcaaccggcg gtgtccgcca gcgccttgcc ctgcacgatg atctccgccg agggctcggc
     5641 ctgcgcataa ccaaggttga acgcggtgca caaaagtatg atcgcgggaa tcgctcgcat
     5701 tgcgcaataa ttgtccgtgc accacatttg cttctctcgg atgctatcac gcaaaatcat
     5761 gccggcgaga gcatcacgtc gtttcgctgg cgcgccatgc gacgacacaa gacgaaaact
     5821 gccggcgccg tttcgggcat gaaattgcga actggggacg cggggctcca ttgcggagat
     5881 ttgtggtgcg ctggccgcgg aaaagcgaca tagactggtt ctaagttgtg aaaacaacta
     5941 gctaaaagag cctcagcgtc gcggctcaaa gaggatggaa tgggaatcaa ccagggtccg
     6001 atcagtctcg atcagaaata cacccaaggg tcgggccacg tcttcctgac cggaattcag
     6061 gcgctggtcc gcctgccgat ggctcagatc cgccgcgacc gcgccgccgg cctcaatacc
     6121 gcgggttttg tgacgggata ccggggttcg ccgctcggcg gctatgacca gcaattgttc
     6181 gccgcgcgaa aacacctcga gcaatacaac atcaaatttc agcccggcgt gaacgaagac
     6241 ctggcggcca ccgctatctg gggctcgcag cagttgaact tgtcgcccgg cgcaagatac
     6301 gatggcgcgg tcggcatctg gtacggcaag ggccccggcg tcgaccgctg cggggacgtg
     6361 tttcgccacg gcaacgccgc gggctcggca aaaaatggcg gcgtgctctg tcttgccggc
     6421 gacgatcacg gcgcaaagtc ttccaccgtg ccgcatcagt cggaccacgc cttcatctcc
     6481 gccttgatgc cctacctcta tccctccagc atccacgaaa tgatcgagat ggggctgctc
     6541 gggatcgcga tgtcgcgcta ttccggctgc tgggtcggca tgaaggtgat caccgagacc
     6601 gtggagacca cggcggaggt cgacctcacc gacgagatga cgccgttcat catccccacc
     6661 gatttcgaga tgccgccggg tggactgaac ctgcgctggc ccgatgaccg ctacgaacag
     6721 gaccggcgcc tgcaggacta caagggcttt gcggcaatcg cattcgcccg cgccaacaag
     6781 gtcaaccgcg tcaccatgga ttcgccgaat gcgcggttcg gcatcatggc ctcgggcaag
     6841 agctacgagg atatccggca ggcgctgcgc gaactcggag tgacggcgga agttgccgcc
     6901 aagatcggcc tgcgacttta caagatcggc atgccctggc cgctggagcc gcagggggtg
     6961 cgggaattcg cggtcggcct cgaagagatt ttcatcgtcg aagaacgccg cgagatcgtc
     7021 gagaaccagg tcaagcagga attgttcaac tggcgcgacg acgtgcggcc gcgcatcgtc
     7081 ggcaagatgg acgagcacga caagcgcttc ctgacattcg cggccgagct gagcgttgcg
     7141 tcgctcgcga gctcgttgac cgagcggctg cttcgcctca acctcaatcc cgaaatcgcg
     7201 gaaatgctgc gcgccaaggc cgactggttc aacggccgtg aggcgaccca gatgcaggcc
     7261 gtggcgccga tcagccggac gccgtatttc tgctcgggct gcccgcacaa cacctcgacc
     7321 aaggtacccg agggcagccg cgccttcgcc ggcatcggct gccacttcat ggcgctgtgg
     7381 atggatcgca gcaccgaaac ctacacccat atgggaggcg agggcgtgcc gtgggtcggc
     7441 gttgccccct ttaccgacga aaaacatgta ttcgccaatc tcggcgacgg cacttatttc
     7501 cactcgggaa gccttgcgat ccgtcaggcc gtctcgtccg gcgccaacat cacctacaag
     7561 atcctctaca acgacgcgac cgcgatgacc ggcggccagc acgtcgacgg cgagctttcg
     7621 ccgcagcaga tcaccttcca gctgcactcg gaaggcattc gcgacatcta tctggtttcg
     7681 gaaaatcccg acgcctatcc ggcctccgac atcgcgcccg gcaccaggac cgcgcatcgc
     7741 gatgaactcg acgcggtgat gaaaacgctg cgcgaagtga agggcgccac cgccatcgta
     7801 ttcgtgcaga cctgtgccgc cgaaaaacgc cgccgccgca aacgcggcac gctggaggat
     7861 ccggcccgcc gcgtcatcat caacccagcg gtgtgcgagg gctgcggcga ttgctcggtg
     7921 caatcgaact gcatctcggt ggaaccgctg gagaccgaac tcgggcgcaa gcgcaccatc
     7981 aaccagtcga gctgcaacaa ggattactcc tgcctgaaag gtttttg
//
LOCUS       DATNOI010000186         8876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_570445,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000186 DATNOI010000000
VERSION     DATNOI010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8876
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570445"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VN968_07160"
     CDS             <1..1124
                     /locus_tag="VN968_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29053.1"
                     /translation="AGLAGKGDIYAADVFHRFVRWVAKVEEDAKHGRQSSVGHWAYYS
                     AHKHRLIENIGALVGQLRSTMSKTKDELDFSYQSLDALSAYVEEIGAERAEREIYDHL
                     VAYVGEVLRLRIQGHWAVNNRDWQPYPYLVGPMHDPVMPINVVQGELSGLAPVNLRLA
                     AANEVRSKRKPSAEVADAATGVRPAAPKGVLASLPGDAYEVTKRWADGRPWIVILKGD
                     IAVAGIPCRDEAAFGRKGDLVCGTLSREWLFGTRRFAANSQFRFYRGREDGRLNDVKL
                     GADQEIDGLPCLGGTVVWFHPNQRVSSLHLASNRDVDSIPCASGDNLILALNFHQNGR
                     LASAVLAREHVVAGQRYERGTLLRFDRDGGLIYAQPYLH"
     gene            complement(1322..1450)
                     /locus_tag="VN968_07165"
     CDS             complement(1322..1450)
                     /locus_tag="VN968_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29054.1"
                     /translation="MIYSMATTTAGPSQFLTLMKLFNLAVLVVVTLYSGAKWLAMK"
     gene            1906..3366
                     /locus_tag="VN968_07170"
     CDS             1906..3366
                     /locus_tag="VN968_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXL29055.1"
                     /translation="MEHRRFLAHPEAHRPFIGGDLLRRIGGRTAVEALVDGLYDRIET
                     DAVLRPLFNRDLANEREAQKRFFAEWLGGDTSYSDRAHLPLHHRHDLLPITRTLAEKW
                     LGHLRDALDSAVSDIEARRAIQDKVRVLAMALVNEGAMPSALRARPHGTCLRYEPAIE
                     SVTLARRGDAAALRRLVQRAPNVLVSAPHAARLLQLAVLGGRKPVVEFLLDAGVDVNK
                     PSPIEPLIFVTPLCAARAKRRADIEALLLRCGAKEDVFTHAFLGDLDRLHEDVGSDPR
                     AAQAIDPAVDALQITPLHHAVAGGRVEALRALLSGVSQANEPLLGGARAVREAVARES
                     VAMVAMLIEHGANAASIGAGRWVLHPELAPMLARAGASIDRSGSWIGLSCTGNQGRKD
                     DPQYVAGLLRHGARVEDSRLTGQRTDGGRATALHYAAKAGFVKTIEVLLEHGADPTAR
                     DDNGFTPLDWVERAAPSIDRQAVRRLLHRPRWSRPP"
     gene            3463..4365
                     /locus_tag="VN968_07175"
     CDS             3463..4365
                     /locus_tag="VN968_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29056.1"
                     /translation="MRIMCRLVSCLVLLFPIATAPAGPARAAVTCPFISDQQLTGAMP
                     ATKWSLISNQDGRGCIFQGARGDTLMLTVFRNPTADRAKELYATFVKTLGERMPVIPV
                     SGIGDTTQAGTTTPDAVRPEASVVALSGEYILSLSVYRSGRPADGTLLKPLTEVARRA
                     IGNVGATSEKFGSCEWLTADDAEGFLDRSTLTIQRTGAESCLIFDGAANTMIVAVIAT
                     SRDTQVNMMNRNSGCKHVPLPEFGREAFGEHSCNSGNTNAVNIFVWKNGKQASILFAP
                     TKPHPESGSVEHLKAVASRVYGKM"
     gene            4514..5179
                     /locus_tag="VN968_07180"
     CDS             4514..5179
                     /locus_tag="VN968_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXL29057.1"
                     /translation="MGALIFDFDGVIADSEAIANTVLAEIVTGLGHSTTLDQALARYS
                     GRRWDEAVAEIEAAIGKPLPADFSSELKRATLDRFRTDLKEVSGAADFIRRFSHIPRC
                     IASSSSIDRLRLCLSVLALEAEFGSHVFSADMVARGKPHPDIFLFAAGKLGVSPDECL
                     VIEDSAGGIKAAVAAGMTAVGLCAASHIREGHDLKLRDAGAVHLAHSWPEVERFARQF
                     FKC"
     gene            5291..6757
                     /gene="radA"
                     /locus_tag="VN968_07185"
     CDS             5291..6757
                     /gene="radA"
                     /locus_tag="VN968_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HXL29058.1"
                     /translation="MAKNTLSFVCQNCGAAYNRWAGKCESCGEWNTLAEEDITGATTM
                     PVSIRSKRKGRLFALETLTGKSVEAPRLPSGMAELDRVTGGGFVRGSVLLVGGDPGIG
                     KSTLLTQATSLMARAGHRAVYISGEEAVAQVRLRAERLGLADAPVQLAAETSVEDIVS
                     TLSEGAVPRLIVIDSIQTMWTDTVESAPGTVTQVRASAQALIRFAKKSGAAIILVGHV
                     TKDGQIAGPRVVEHMVDAVLSFEGEGSQQFRILRAVKNRFGPTDEIGVFEMTGLGLRE
                     VSNPSELFLSERDLGSPGTAVFAGIEGTRPVLVELQALVAPTSLGTPRRAVVGWDPSR
                     LSMVLAVLEAHCGVKLSGHDVYLNVAGGLRIQEPAADLAAAAALVSSLVNAPLPTDAV
                     YFGEISLSGAVRPVAQTSARLKEAAKLGFGRAVLPESARGDVGGDAGLVLNTVGGLTS
                     LVADIAARGSPRGNREGNRESTAVEKNATPARFRRQES"
     gene            6921..7550
                     /locus_tag="VN968_07190"
     CDS             6921..7550
                     /locus_tag="VN968_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HXL29059.1"
                     /translation="MPITILDLLLLAVMLISGLLAMVRGFMREILSIAAWGTAAVVTL
                     YAFSKLLPTAKTYFNNDTVATVVVVAGVFVGTLILVSIVTVRISDMILDSRIGALDRT
                     LGFLFGLARGLLIVVVAFLFFSWLVPDKQRPDWVTGAKSRVVLQGTGDWLMSLLPDDP
                     ENTILKRFKKNKPDDEQTDTDPAAPATGDGYTKPARDSLKKLIEKPAAR"
     gene            7574..>8876
                     /gene="purF"
                     /locus_tag="VN968_07195"
     CDS             7574..>8876
                     /gene="purF"
                     /locus_tag="VN968_07195"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXL29060.1"
                     /translation="MDCQQSPSDEAAQYDLNLDPPPTPDLLDSDLQDSDLEGDTLREE
                     CGVFGIFGHPDAAAITALGLHALQHRGQEAAGIVSFDGGRFHSERRLGLVGDTFSRRA
                     VIDRLPGTSAVGHVRYSTTGETILRNVQPLFAELNAGGFAVGHNGNLTNGLTLRRELV
                     RNGAMMQSTTDTEVILHLVAQSKRNRFIDRFIEALRAIEGAYSLVSLTNKKLIGARDP
                     LGIRPLVLGELDGCPILASETCALDIIGAKYVRDVNPGEVVVFDEHGSQSHKPFPPMP
                     PRPCIFEYIYFSRPDSIVGGRSVYDVRKAMGAELARESHIEADVVVPVPDSGVPAALG
                     YSRESGLPFELGIIRNHYVGRTFIQPTQSVRELGVRMKHNANRAAIEGKRIILIDDSL
                     VRGTTSKKIVRMMRDAGAREVHMRIASPPITHSDYYGIDTPE"
BASE COUNT         1584 a         2810 c         2812 g         1670 t
ORIGIN      
        1 tcgcggggct tgcaggaaag ggcgacatct atgcggccga cgtctttcac cggttcgtgc
       61 gctgggtggc caaggttgag gaagacgcca agcatgggcg ccagagctcc gtcgggcatt
      121 gggcgtacta ctcggcgcac aaacatcgtc tgatagaaaa tatcggcgcg ctggtcgggc
      181 agctccgttc gacgatgtca aagacgaaag acgagctcga cttctcgtat caaagtcttg
      241 acgccttgtc cgcgtatgtc gaagaaatcg gggccgagcg ggccgagcgg gaaatctacg
      301 atcacctggt ggcctatgtc ggcgaagtgc tgaggctgcg tattcaaggg cattgggcgg
      361 tcaacaaccg cgattggcag ccctatccat atctggtggg accgatgcac gatcccgtca
      421 tgccgatcaa cgtggttcag ggagagctct caggcctcgc gccggtcaac ctgaggctag
      481 ccgctgcgaa cgaggtccgc agcaagcgca agccgtcggc agaggtcgcc gatgcggcga
      541 ccggcgttcg cccggccgcg cctaagggcg tgctggcgag ccttccaggg gatgcctacg
      601 aagtcaccaa acgatgggcg gatggccggc cgtggatcgt catcttgaag ggcgacattg
      661 cggttgccgg catcccctgc cgagatgagg ccgcgttcgg ccgcaagggt gatctcgtct
      721 gcggcacgct gtcccgcgag tggttgttcg gtacgcggcg attcgccgcc aattctcagt
      781 tccgtttcta tcggggacgc gaggatggac gcctcaatga tgtgaaattg ggcgcggatc
      841 aagagatcga tggcctgcct tgccttggcg gaacggtggt gtggtttcac cccaaccagc
      901 gcgtcagctc tctccatctg gcaagcaatc gcgatgtcga tagtattccc tgcgccagcg
      961 gtgacaattt gattctggcg ttgaacttcc atcagaacgg gcgcctcgcg tctgccgtgc
     1021 tggcgagaga acacgtcgtc gccggccagc gctacgaacg ggggacgctg ctgcgtttcg
     1081 atcgggatgg tgggctgatt tatgcccagc cgtatttgca ctaagtcctt caacgtcgtt
     1141 gtcgctgcaa gcggactata tgaagtctcc ggcaggcggt tgcttcgcaa tgctctgttt
     1201 ctcgcttagt tgtttttctt tccagcgact ggacggtggc agtctcgcct cgtgccggta
     1261 tgctgcacgg cctgcttacg gcaagccgtc cgcacattcc gcgcgggtcg gttccagcaa
     1321 actatttcat tgccagccat ttcgcgcccg aatagagcgt tacgaccacc aggacggcga
     1381 gattgaaaag cttcatgagc gtaaggaact gactcggccc tgccgtcgtg gtcgccatgc
     1441 tgtagatcat ccaactagac cccgctagac ctaaaagaaa taccagtcca cggcgtggag
     1501 tgccaggggc ctgtttgact ttgcctccaa cgttttggaa attaggccgc cggtttttgg
     1561 ccattcgatc accccgtcca ttgccgccat taccatagcg tggtccatag catggtccgc
     1621 tacgggctgc tttgcaccag gcgaaacgac gataggacgc agccgaactg aatagcattt
     1681 catgaagttg gtgtggcttt tcgtgcgaca aaaaccccgg atattatagt cggcacaatg
     1741 ccgatgtgga aatgcgtttc atcgctgtgc tgatccctcc ccatcgcaat atgccgtgag
     1801 caaaggcgtg catcgtcacc gtaattccag ccgcctgctt gatccgaaga cggtgaaaag
     1861 gtaactgact tctcgatcag aacacctgcc gaacgcgagc gctcgatgga gcaccgtcga
     1921 tttcttgccc acccagaggc gcaccgcccc ttcatcggtg gcgatctcct ccggcgcatc
     1981 ggaggccgta ccgcggtcga agcgctcgtc gacgggctct atgaccgcat cgagaccgac
     2041 gccgtgctgc ggccgctctt caaccgcgat ctcgccaacg agcgcgaggc gcagaagcgc
     2101 ttcttcgccg agtggctcgg cggcgatacg agctacagcg accgcgccca cctgccgctc
     2161 catcatcgcc acgatctgct gcccatcacc cgcacgctcg ccgaaaagtg gctcgggcat
     2221 ctccgcgacg cgctcgactc ggcggtgtcg gacatcgagg cgcgccgcgc tatccaggac
     2281 aaggtccggg tcctggcgat ggcgctggtc aacgagggag cgatgccttc ggcgctgcgc
     2341 gcgcgaccgc acggtacgtg cttgcgttac gagccggcga tcgagtcggt caccctcgcc
     2401 cggcgcgggg atgccgcggc gctgcgtcga ctggtgcagc gcgcgccgaa cgtcctcgtc
     2461 tccgcgccgc atgccgcacg tctcctgcaa ctcgccgtgc tcggcggacg caagcctgtc
     2521 gtcgagttcc ttctggacgc tggggtcgat gtgaacaagc cctcgccgat cgagccgctc
     2581 atcttcgtaa cgccgctgtg tgcggcacga gcgaagcggc gcgccgacat cgaagcgctc
     2641 ctgcttcgct gcggtgcgaa ggaggatgtc ttcacgcacg cgttcctcgg agacctcgat
     2701 cgtctccacg aggacgtcgg cagcgaccca cgggcagcgc aggccattga tcccgccgtc
     2761 gatgctctcc agatcacgcc gctgcatcat gccgtggctg gcggccgggt ggaagcgttg
     2821 cgcgcgcttc tctcaggcgt ctcgcaggcc aatgagcccc tgctcggcgg tgcccgcgcg
     2881 gtccgcgaag cggtggcgcg cgagagcgtg gcgatggtcg cgatgctcat cgaacacgga
     2941 gcgaacgcgg cgtcgatcgg cgcgggccgc tgggtcctcc atcccgagct ggcgccgatg
     3001 ctcgcgcgcg cgggggcgag catcgatcga tcggggtcgt ggatcgggct ctcgtgcacc
     3061 ggcaaccaag gacgaaagga tgacccccag tatgtcgcgg ggctgctgcg tcacggcgcg
     3121 cgcgtcgagg acagccgcct cacggggcag cggaccgacg gcgggcgagc cacggcgctc
     3181 cattacgctg ccaaggcggg tttcgtgaag accatcgagg ttcttctcga gcacggcgcc
     3241 gatccgaccg cccgagacga caacgggttc accccgctcg attgggttga gcgggctgca
     3301 ccatcgatcg atcgtcaggc agttcgacgc ctgcttcacc gtccgcgctg gtcccgacct
     3361 ccgtgatggt gtgaaggcgc tcatcttcat tcaccgttac gcgtacctcg acatcggggc
     3421 cggcgaaaat tcccggttcg tccacgacag cggaaaagtc caatgcgcat catgtgtcgc
     3481 ctcgtctctt gcctcgtcct gctttttccg atcgccacgg cgccggccgg tccggcgcgc
     3541 gcggccgtga cgtgcccgtt catcagcgac cagcagctca ccggcgcgat gccggcgacc
     3601 aaatggtcgc tcatttcaaa ccaggacggc cggggctgca ttttccaggg cgcgcgcggc
     3661 gacacgctga tgctcacggt gttccgcaat ccgacggcgg accgcgccaa ggaattgtac
     3721 gcgacgtttg tcaagacgct cggggagcgc atgccggtca tcccggtgtc cgggatcggc
     3781 gacacaacac aggcggggac gaccacgcct gatgccgtgc gtccggaagc gtcggtcgtg
     3841 gcgctgtccg gggaatacat cctgtcgctc agcgtctacc gatccggccg gcccgccgac
     3901 ggcacactct tgaaaccgct gaccgaggtt gcgcggcgcg caatcggcaa tgtcggcgcg
     3961 acgagcgaga aattcggcag ctgcgaatgg ctcacggccg acgatgccga gggatttctc
     4021 gaccggagca cgctgacgat ccagcgcacc ggcgcggaga gctgcctgat cttcgatggc
     4081 gccgccaata ccatgatcgt cgccgtgatc gcgacgtcgc gcgacaccca ggtcaacatg
     4141 atgaaccgca acagcggatg caagcacgtg ccgttgcccg agttcggcag agaggccttc
     4201 ggcgaacatt cctgcaacag cggcaacacc aatgcggtca atatttttgt ctggaagaat
     4261 ggcaagcagg cgtcgatcct gttcgcaccc accaagccgc atcctgaatc cggatccgtc
     4321 gagcacctga aagccgtcgc cagccgggtg tacggaaaaa tgtagtcggc ggcgaaggcg
     4381 gcctaaccag cggtgcctga tcgacacacc atgcaacaga aagtgttcga ctgctttcgg
     4441 ttgcatcggt ccattttggt gccggaccgc atattaagca tgcgaccctc tggtcttccc
     4501 cggaattgaa ataatgggtg cattgatttt tgacttcgac ggcgtcatcg ctgacagcga
     4561 ggcgatcgcg aacaccgtgc tggcggaaat cgtcaccggc ctcggtcatt caaccacgct
     4621 tgaccaggca ctcgcgcgct attccggccg aaggtgggat gaagccgtgg ccgagatcga
     4681 agcggctatt ggaaagccgc tgcctgccga tttttccagc gaactcaagc gcgcgacgct
     4741 ggacaggttc cggacggatt tgaaggaagt cagcggagcc gcagatttca tcagacgttt
     4801 ttcccacatc ccccgatgca tcgcatcatc cagttcgatc gatcgtctgc ggctgtgcct
     4861 ttcggtgttg gcattggaag cggagttcgg cagtcatgtt ttcagtgccg atatggtggc
     4921 gcggggaaag ccgcatcccg acatctttct gtttgccgcc ggcaaattgg gcgtcagccc
     4981 ggatgaatgt ctcgtgatcg aggacagcgc gggcggcatc aaggcggccg ttgcagcggg
     5041 gatgacggcg gttggcttgt gtgccgccag tcatattcgc gagggccacg atctcaagct
     5101 gcgcgatgcc ggcgccgtgc atttggcgca ttcgtggccg gaggtcgagc gcttcgctcg
     5161 ccaatttttc aaatgttagg gtgaggttcg gtattccggc aatctgcgcc atccgttggt
     5221 atcatgctgg cggctgatct ttccatgcta tgcaacggct tatccggatt aaacagcgac
     5281 tcactccccc atggccaaga acaccctctc ctttgtctgc cagaactgtg gcgcggcgta
     5341 taaccggtgg gctggcaagt gcgaatcctg cggcgagtgg aatacgctgg ccgaggagga
     5401 tatcaccggc gcaaccacga tgccggtctc gattcgctca aaacgcaagg gccggctgtt
     5461 cgcgctggaa accctcaccg gcaaaagcgt tgaagcccct cgcctgccct ccggcatggc
     5521 cgagctcgac cgcgtcaccg gcggcggttt tgtgcggggc tcggtgctgc tggtcggcgg
     5581 cgatcccggc atcggcaaat cgacgctgtt gacgcaggca acctccctga tggcgcgggc
     5641 cggccatcgc gcggtgtaca tttcaggcga ggaagcggtg gcgcaggtgc ggctgcgcgc
     5701 cgagcggctt ggcctcgccg acgcgccggt gcagctcgcc gcggaaacca gcgtcgagga
     5761 catcgtttcc accctgtccg agggcgcggt gccgcggctg atcgtgatcg attcgatcca
     5821 gaccatgtgg accgacacgg tggaatcagc accgggcacg gtgacgcaag tgcgcgcctc
     5881 ggcgcaggcg ctgataagat ttgccaagaa atccggtgcg gccatcatcc tggtcggcca
     5941 tgtgacaaaa gacggccaga tcgcaggtcc ccgcgtggtc gagcacatgg tcgatgcggt
     6001 gctgtcgttc gagggcgaag gctcgcagca gtttcgtatt ctgcgcgcgg tgaagaaccg
     6061 gtttgggccg accgacgaaa tcggcgtgtt cgagatgacg ggcctcggct tgcgcgaggt
     6121 ctccaaccct tccgaattgt tcctgtccga gcgcgatctc ggcagtccgg gcaccgcggt
     6181 attcgccggc atcgagggca cccgtccggt gctggtggaa ttgcaggcgc tggtggcgcc
     6241 gacctcgctc ggcaccccgc gccgcgccgt ggtcggctgg gatccgagcc ggctctcgat
     6301 ggtgctggcg gtgctggaag cccattgcgg cgtcaaactg tccggccatg acgtttattt
     6361 gaacgtggcg ggcggcctgc ggatccagga gcccgcggcc gacctcgccg ccgccgccgc
     6421 cctggtctcc tcgctggtca acgcgccgct gccgaccgac gcggtctatt tcggcgagat
     6481 ttcgctgtcg ggcgcggtcc ggccggtggc gcaaacctcc gcgcggctga aggaagccgc
     6541 caaactcgga ttcggccgcg ccgtgctgcc cgaatcggcg cgcggggatg tcggcggcga
     6601 cgccggcctt gtgctcaata ccgtcggcgg attgaccagc ctggtcgcgg atatcgccgc
     6661 tcgcggttcc cccagaggca atcgagaagg aaaccgcgag agcacagcgg tggagaaaaa
     6721 tgcaacacct gcgagattcc ggcgccagga gagctagccg gacgtgacct tacctttgca
     6781 cgccgctata ccacgcgcgc gcaagacgga gattgcacac ggtttccgac gttgcgggac
     6841 atgccatatc accgtcaccc tagggcggca ccgacacgcc gattcgcggt tttgggcttt
     6901 tgagcggacc tgactagccg atgccgataa cgatacttga cctcctcctg ctcgccgtga
     6961 tgctgatttc cggcctgctg gcgatggtgc gcggcttcat gcgcgagatc ctgtcgatcg
     7021 cggcctgggg tacggccgcg gtggtcacgc tttatgcatt ctcgaagctg ctgccgaccg
     7081 ctaaaaccta tttcaataac gatacggttg cgaccgtggt cgtggtcgcc ggcgtgtttg
     7141 tcggtaccct aatcttggtt tccatcgtca cggtgcggat ttccgacatg atcctggatt
     7201 cccggatcgg ggcgctcgac cgcaccctcg ggttcctgtt cggactggcg aggggccttt
     7261 tgatcgtcgt cgtcgccttc ctgttcttca gctggctggt tccggacaag cagcggccgg
     7321 actgggtcac cggggccaaa tcccgggtgg tattgcaggg aactggggat tggctgatgt
     7381 cgctcttgcc ggatgacccc gagaacacca tcttgaagag attcaagaag aataaaccag
     7441 atgacgaaca aactgacacc gacccggcgg cgccggctac tggtgatggt tataccaagc
     7501 ctgcccggga cagccttaaa aagctgatcg agaagcccgc ggcacgttga gatcaggcca
     7561 aaagagaggc gcgatggact gtcaacagag cccttccgat gaagccgcac aatacgatct
     7621 gaacctcgac ccaccaccta ccccggactt gctggattcc gatctgcagg attctgacct
     7681 tgaaggcgac acgttgcgcg aggagtgcgg tgtgttcggc atctttggcc accccgatgc
     7741 cgccgcgatc acagccctcg gattgcacgc tttgcagcat cgcggccagg aggccgcggg
     7801 catcgtttcc ttcgatggag gccggtttca cagcgagcgg cggcttggcc tcgtcggcga
     7861 caccttttcc cgccgcgccg tgatcgaccg gctgccgggc acatcagcgg tcggccacgt
     7921 gcgctattcc acgaccggcg aaaccatcct gcgcaacgtg caaccgctgt tcgccgagct
     7981 caatgccggc ggatttgcgg ttggacacaa cggcaatctc accaacgggc tgaccctgcg
     8041 ccgtgaactg gtgcgcaacg gcgccatgat gcaatccacc accgacaccg aggtcatcct
     8101 gcacctggtg gcgcagtcca agcgcaaccg cttcatcgac cgcttcatcg aagcgctgcg
     8161 cgcgatcgaa ggcgcctact cgctggtgtc gctgaccaac aagaagctga tcggcgcccg
     8221 cgatccgctc ggcattcgcc cgctggtgct gggcgaactc gacggctgcc cgattctggc
     8281 gtcggaaacc tgcgcgctcg acatcatcgg cgccaaatac gtccgcgacg tcaatccggg
     8341 tgaggtcgtc gtgttcgacg agcacggcag ccagagccac aagccgtttc cgccgatgcc
     8401 gccgcggccc tgcatcttcg aatacatcta tttctcgcgg ccggattcga tcgtcggcgg
     8461 ccgctcggtc tatgacgtgc gcaaggcgat gggcgccgaa ctcgcccgcg agagccatat
     8521 cgaggccgac gtggtggtcc cggtgccgga ttccggcgtg ccggcggcgc tcggctactc
     8581 cagggaatcc ggcctgccgt tcgagctcgg catcatccga aaccattacg tcggacgcac
     8641 cttcattcag ccgacccaga gcgtgcgcga actcggagtg cgcatgaagc acaatgcgaa
     8701 ccgcgccgcg atcgagggca agcgcatcat cctgatcgac gattcgctgg tgcgcggcac
     8761 cacctcgaaa aagatcgtcc gcatgatgcg cgacgccggc gcccgcgagg tgcacatgcg
     8821 catcgccagc cccccgatca cccattccga ttattacggc atcgacacac ccgagc
//
LOCUS       DATNOI010000187         8962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1213209,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000187 DATNOI010000000
VERSION     DATNOI010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8962
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213209"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2016)
                     /locus_tag="VN968_07200"
     CDS             complement(<1..2016)
                     /locus_tag="VN968_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXL29061.1"
                     /translation="MNILPGNMRFGAGQPVKRLEDQRLLTGKGLFIDDRPEDGALWLH
                     VLRSPHAHANIVSIDIDATREMPGIEAVYTGADLVADDIGTLPTLLVFKRPDGSSMTA
                     PPRRLLAHEIVRFAGEPVAAVVATSRYAAQTAAEAIVVEYDVLPSVVDPVAATRPGAP
                     AVWAEAPDNIVAAMSYGDAAAVEAAFAGAAHVVSLDLISQKLIPSAMEPRSTIAEIEK
                     KTGRLILHVQSQTPGSTRDLLAEAVLKRPKESVRVLVGDIGGGFGQKTSLYPEDGMVA
                     YAAVKLNRKVRWRGDRTDEFVGGTHGRDLTSTGEFALDAKGRVLAYRVRSLGGTGAYL
                     TGTGAIIPLVLGPFVQSGVYDLPLVHYEVKAVMTNTAPVGAYRGAGRPEGVFIAERLM
                     DAAARQIGMDPRAIRKINYIKPAQLPYTNAVGQVYDSGAFAHMLERASKLADWDGFAT
                     RKKAARKKGLLYGRGLTSYIEWTGGRAHTEKVSLHATAEGRIILHSGTQAMGQGSQTT
                     YSQMIAESLGIAMDKIDIVQGDTDLATGFGSVGSRSLFVGGTAVAVSSNDLIAKAREK
                     ASHLLEASVEDIEYRDGMLTVVGTDKRIGLFDIARHEKGARLSVDSEGEVDGPSWPNG
                     THICEVEIDPETGVSKVVRYTTVDDVGVAVNPMLATGQVHGGVAQGIG"
     gene            complement(2275..3150)
                     /locus_tag="VN968_07205"
     CDS             complement(2275..3150)
                     /locus_tag="VN968_07205"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXL29062.1"
                     /translation="MADFHGVFPYLVSPVDADGKVRTGVLGRLCDDLIKSGVHGLTPL
                     GSTGEFAYLNNAQRRAVVQTTIEAAKGRVPVVAGVASTATADAVAQAKFYETLGADGI
                     LAILEAYFPLGDAQVESYFRAIADAVDIPVVIYTNPQFQRSDLTLDVIARLAAHPRIG
                     YIKDASTNTGRLLSIMNRCGDAIRVFSASAHIPAAVMLIGGVGWMAGPACIVPRQSVE
                     LYNLCKAARWDEALTLQRRLWRVNEAFARYNLAACIKAGLAIQGYDVGDPIPPQAALS
                     ADERKAVEAVLREIG"
     gene            complement(3143..3634)
                     /locus_tag="VN968_07210"
     CDS             complement(3143..3634)
                     /locus_tag="VN968_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29063.1"
                     /translation="MSRDGGARQKADVDAVYTFGMPRPGSKAFADIYSQQLGTRTYRL
                     VHGEDVVPTVAPTSFGFRHLGRVLHCERQGRFDQANLTSDTSSDFPPFVSGVAKDLWD
                     HLHEPLSAVLSIAARIKLATALLTGMGPSNMRTDPGGILIEMLPPRLRDHMPDRYIGA
                     CNG"
     gene            complement(3624..4838)
                     /gene="rocD"
                     /locus_tag="VN968_07215"
     CDS             complement(3624..4838)
                     /gene="rocD"
                     /locus_tag="VN968_07215"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HXL29064.1"
                     /translation="MSSTVADLIATEARLGAQNYEPLGVVLSRGEGVWVWDTQGNRYL
                     DCLSAYSAVNQGHCHPKILAAMVEQAGRLTLTSRAFHNDQLASFYEQIAELTGSHKVL
                     PMNSGAEAVESAIKSVRKWGYEVKGVPDGQAEVIVCADNFHGRTLGIVGFSTDAAARE
                     HFGPFAPGFRLIPFGNARALEEAITPNTVAFLVEPIQGEAGVIIPPSGYFAKVRELCT
                     AHDVMLILDEIQTGLGRTGKLLAEQHDGIEADVTLLGKALSGGFYPVSAVLSNKAVLG
                     TLKPGQHGSTFGGNPLACAVARAALRVLVEEGMIENAAVQGERFLDGLKSIRTNTIRE
                     VRGRGLMLAVELHAEAGDARRYCEALQGRGILAKDTHGHTIRIAPPLVITADQVDWAL
                     ERIAATLRHDFP"
     gene            complement(4956..5582)
                     /locus_tag="VN968_07220"
     CDS             complement(4956..5582)
                     /locus_tag="VN968_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29065.1"
                     /translation="MLVGIGWLDAGKVEQWRRGQIDYLERVVQTNLPRISEAMKLFRS
                     WATRRGLSASPTSYIARSPRRQTLRFSRSGDPTIEALYRTHWVSQELSERKRQHLAEK
                     ASRAPDLVVVQPLNREWKCHRCGKTGDLLMMENPGPACLSCVGLDDLEFLPAGDALLS
                     RRVKAKSTRCAVVVRFSRTRRRYERQGLLVEPLALADVQHDLKLQRRE"
     gene            complement(5925..6530)
                     /locus_tag="VN968_07225"
     CDS             complement(5925..6530)
                     /locus_tag="VN968_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29066.1"
                     /translation="MALKRGRKAQRRKQVVAQKRRAEISEASLPARVLRAAQAPIQHC
                     LLTESVFDVGMGTLVLARGATPHHVALSSFLIDTFCLGIKDVMFESVDSEVFEMYMDA
                     TDAGSPIVSVDPSYARKLLRDLAAWSQSIGFAPHRDFAAVERMFGDVSADASDAVFRF
                     GRDGKPVYIPGPNDTAPLIQRRIEQLQKHLGDDGFGFETAA"
     gene            6792..7076
                     /locus_tag="VN968_07230"
     CDS             6792..7076
                     /locus_tag="VN968_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29067.1"
                     /translation="MEKRFTRDTHRARLAWIRAKMFIGSSGGVTERNMAIDVSEEIAR
                     KGQELREMTRNLDLLKVDVDTIAIVIRFRGASAPTGVQGEDATEGDPERP"
     gene            complement(7259..7831)
                     /locus_tag="VN968_07235"
     CDS             complement(7259..7831)
                     /locus_tag="VN968_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HXL29068.1"
                     /translation="MNQSTPTPPPDWLVTALDAHRQWVAEKYAQQPGATASGPPGMRA
                     TPSQLRLHDVDLTDVAMPGAILAGAVLTRIGLAGAHLEAVDFSDARLTEVDLTGVRAD
                     RATFYDAVLTNCVLARAGLSGADLAAASLHGCRLAGADLTGAALTKAQLYECDLTEAK
                     LDRARAYRASIIGGRSPGRAWLAPTCATLC"
     gene            8442..8684
                     /locus_tag="VN968_07240"
     CDS             8442..8684
                     /locus_tag="VN968_07240"
                     /inference="COORDINATES: protein motif:HMM:NF023494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HXL29069.1"
                     /translation="MRTLFLALATSATVFATGVTEVAAGDYRYCIQGDDFGAGDCSFA
                     SYQQCQATASGRTAYCGANLRFPNVVLTVRTRLRHH"
     gene            complement(8652..>8962)
                     /locus_tag="VN968_07245"
     CDS             complement(8652..>8962)
                     /locus_tag="VN968_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29070.1"
                     /translation="AVYGPVCTVVWEGWSREAPPYPDHWRFSDAGRRSAWIASSRRHP
                     KTCTQAEVKRHAITSTTLTDIVQSVRMKSWITAMARSGASPSLPGSTPLVMTQPSSNC
                     "
BASE COUNT         1629 a         2909 c         2776 g         1648 t
ORIGIN      
        1 tccgatgccc tgcgcgacgc cgccgtgaac ttgccccgtc gccagcatcg gattgacggc
       61 gacgccgacg tcgtcgaccg tggtgtagcg aacgaccttg ctgacgccgg tctccggatc
      121 gatctcgact tcgcagatat gggtgccgtt gggccagctc ggcccgtcga cctcgccttc
      181 gctgtcgacg ctcaggcggg cgcccttttc gtgcctggcg atatcgaaca ggccgatccg
      241 cttgtcggtg ccgaccaccg tgagcatgcc atcgcgatat tcgatgtcct cgaccgaggc
      301 ctccagcagg tgcgaggcct tctcgcgcgc tttcgcgatc aggtcgttgc tcgataccgc
      361 taccgcggtg ccgccgacga acagcgagcg cgaaccgacg ctgccgaatc cggtggcgag
      421 gtcggtgtcg ccctggacga tatcgatctt gtccatggca atgccgagcg actcggcgat
      481 catctgcgaa taggtggtct gcgacccctg ccccatggcc tgcgtgcccg aatgcaggat
      541 gatgcgccct tcggctgtgg cgtgcaggct gaccttttcg gtatgcgcgc gcccgccggt
      601 ccattcgata tagctggtca ggccgcggcc gtagagcagg cctttcttcc tggccgcctt
      661 cttgcgagtg gcgaagccat cccagtcggc aagttttgac gcgcgctcca gcatgtgcgc
      721 gaaggcgccg ctgtcgtata cctggccgac ggcgttggtg tagggcagtt gtgcgggctt
      781 gatgtaattt atcttgcgaa tggcgcgcgg gtccatcccg atctgccgcg ccgcggcgtc
      841 catcagccgc tcggcgatga acacgccctc ggggcggccc gcgccgcgat aggcgccgac
      901 cggtgcggtg ttggtcatca cggccttgac ctcgtaatga accagcggca ggtcatacac
      961 gccggactgc acgaacgggc cgagcaccaa gggaatgatg gcgccggtgc cggtgagata
     1021 ggcgccggtg ccgccgagcg agcgcacccg ataggccagc acgcggcctt tggcgtcgag
     1081 cgcgaattcg ccggttgaag tgaggtcgcg gccatgggtg ccgccgacga actcatcggt
     1141 gcggtcgccg cgccagcgca ccttgcggtt aagcttgacc gcggcgtagg ccaccatgcc
     1201 gtcttccgga tagaggctgg tcttctggcc gaaaccgccg ccgatatcgc cgaccagcac
     1261 gcgaacgctt tccttcggcc gcttcagcac agcttcggca agaaggtcgc gggtcgagcc
     1321 cggcgtctgc gattggacgt gcaggatcag ccgtccggtc tttttctcga tctcggcgat
     1381 ggtgctgcgc ggctccatcg cggaagggat cagtttctgg ctgatgagat cgagcgagac
     1441 cacatgcgcg gcgccggcga acgccgcttc caccgcggcc gcgtcgccat agctcatcgc
     1501 ggcgacgatg ttgtcgggcg cttccgccca taccgccggc gcgccgggcc tggtggcggc
     1561 aaccggatcg accaccgacg gcagcacgtc atattcaacc acgatcgctt ccgcggcggt
     1621 ctgcgccgcg tagcgcgatg tcgccaccac ggcagcgacc ggctcgccgg caaaacgtac
     1681 gatttcgtgg gccagcagcc ggcgcggcgg cgccgtcatc gacgagccgt cgggccgctt
     1741 gaaaaccagc agcgtcggaa gggtgccgat gtcgtcggcg accagatcgg ctccggtgta
     1801 aaccgcctcg atgccaggca tttccctggt ggcgtcaata tcgatcgaca cgatgttcgc
     1861 gtgggcatgc ggcgagcgca gcacatgcag ccacagcgcg ccgtcttccg gtctgtcgtc
     1921 gatgaacagg cccttcccgg tgagaagtcg ctgatcttcg agccgcttga cgggctggcc
     1981 cgcgccgaaa cgcatgttgc cgggaagaat attcatcagt ttgtccttcg agtctcttgt
     2041 tcagtcgctg cgttctagcc gatacgcaag gaacaggcca accaacgttt tgatgtccgc
     2101 ggcgttcaaa aaatcgtcat ggccgggctt gtcccttgct ccgtcattcc gggatgcgcc
     2161 ttcaggcgca gacctcagat gtgcaattgc acatcgggga atctcgagct gatcatctct
     2221 ggattccggg ttcgcgctgc gcgcgccccg gaatgacgga cggcaggcga actgtcaccc
     2281 gatctcccgc aacaccgctt cgaccgcctt gcgctcatcc gcgctcaatg cggcctgcgg
     2341 cggaatggga tcgccgacgt cgtagccctg gatcgcaagc ccggccttga tgcaggccgc
     2401 cagattgtag cgcgcgaagg cttcgttgac gcgccacagc cgccgctgca acgtcaacgc
     2461 ctcgtcccag cgcgcagcct tgcagagatt gtaaagctcc acgctctggc gcggaacgat
     2521 gcaggccggt cccgccatcc accccacgcc gccgatcagc atcaccgcgg cggggatatg
     2581 cgcggaggcc gagaacacgc ggatggcgtc gccgcagcgg ttcatgatcg acagcagccg
     2641 tccggtattg gtggaggcat ccttgatgta gccgatccgc ggatgggcgg cgagccgcgc
     2701 gatcacgtcg agcgtcaggt cggagcgctg aaactgcgga ttggtgtaga tcaccacggg
     2761 aatatcgacc gcatccgcga tggcgcgaaa ataggattcg acctgcgcat cgccgagcgg
     2821 gaaatatgct tcgaggatcg ccagaatgcc gtcggcgccc agcgtctcat aaaacttcgc
     2881 ctgcgcgacg gcatcggccg tcgcggtcga tgcgacgccg gcgaccaccg gcacgcggcc
     2941 ctttgccgct tcgatcgtgg tctgcaccac cgccctgcgc tgcgcgttgt tgaggtaggc
     3001 gaactcgccg gtcgatccga gcggcgtcag cccatgcacg cccgacttga tgagatcgtc
     3061 gcagagacgg ccgagcacgc cggtgcggac ttttccgtcg gcatcgaccg gcgagaccag
     3121 atagggaaag acgccgtgga aatcagccat tgcaggcgcc gatgtagcgg tccggcatgt
     3181 gatcgcgcag ccgcggcggc agcatctcga tcagaattcc tccgggatcg gtacgcatgt
     3241 tcgaaggtcc catgccggtc aggagcgccg tggccagttt gatccgcgcc gcgatcgaca
     3301 gcaccgcgga aagcggctca tgtaaatgat cccagaggtc cttggcaacg ccgctgacga
     3361 aggggggaaa gtccgaactc gtatcggagg tcagattggc ttgatcgaac ctgccctgtc
     3421 gttcgcagtg cagaacccgt ccaagatgcc tgaaaccaaa cgaagtgggt gcgacggtcg
     3481 gcacgacatc ttcgccgtgc accagccgat aggtccgcgt ccccaactgt tgactgtata
     3541 tatccgcaaa cgccttgctg cccgggcgcg gcatgccgaa cgtgtagacg gcatcgacgt
     3601 cagccttttg ccgggcaccg ccatcacggg aaatcgtgcc tcaaggtggc tgcgatccgc
     3661 tccagcgccc agtcgacctg atcggcggtg atcacaagtg gcggcgcgat gcggatcgtg
     3721 tggccatggg tgtccttggc aaggatgcct ctgccctgca gtgcctcgca gtaacgacgg
     3781 gcatcgcccg cttccgcgtg aagctcgacc gccagcatca gcccgcgtcc gcgcacctcc
     3841 cggatcgtgt tggtgcggat gctcttcagg ccatcgagga agcgctcgcc ctggacggct
     3901 gcgttctcga tcatcccttc ctcgacgagc acgcgcaggg ctgcccgcgc caccgcgcag
     3961 gccagcgggt tgccgccgaa ggtcgagccg tgctggccgg gtttcagggt gccgagcacc
     4021 gccttgttcg aaagcacggc cgacaccgga tagaaacctc cggacaatgc cttgccgagc
     4081 agcgtcacgt ccgcctcgat gccgtcgtgc tgctcggcga gcagcttgcc ggtgcggccg
     4141 agcccggtct ggatctcgtc caggatcaac atcacgtcgt gcgcggtgca gagttcgcgg
     4201 accttcgcaa agtagcctga tggcgggatg atgacgccgg cctctccctg gatcggttcg
     4261 accaggaagg cgaccgtgtt cggcgtgatc gcctcctcca gcgccctggc gttgccgaac
     4321 gggatgagcc gaaagcccgg cgcgaacggc ccgaaatgct cgcgtgccgc cgcgtcggtg
     4381 ctgaagccga cgatcccgag ggtgcgcccg tgaaaattgt cggcgcagac aatgacctcg
     4441 gcctgcccgt ccggcacgcc cttcacctcg tagccccact tgcgcaccga cttgatcgcg
     4501 ctctccaccg cctcggcgcc gctgttcatg ggcagcacct tgtgcgagcc ggtcagctcc
     4561 gcgatctgct cgtagaagga agccagttgg tcgttatgaa aggcgcggga cgtcaacgtc
     4621 agcctacccg cctgctccac catggcggcc aggatcttcg gatggcagtg gccctggttg
     4681 acggccgaat aggcggagag gcaatcgaga taacggttgc cttgcgtatc ccacacccag
     4741 accccttcgc cgcgcgacag aaccacgccg agcggctcgt agttctgcgc gcccaggcgc
     4801 gcttccgtcg cgatcaaatc ggcaactgtt gagctcatct ttcgtcactc cagttgcagg
     4861 tcgcagcggg tcgtgtcctt gggcatcatc ctgcccgcaa tgcccgcggt cgggcaggta
     4921 gactttggcc tatcgaggaa gtccacggta ggacattact cgcgtcgctg caatttgagg
     4981 tcgtgctgca cgtccgccaa cgccagcggt tccaccagca gaccttgtcg ttcgtagcgg
     5041 cggcgcgttc ggctgaagcg cacgacaaca gcgcatctcg tgcttttcgc cttcacccgc
     5101 cgcgacagca gcgcatcgcc cgccggcaag aactcgagat catcgagacc aacgcaggac
     5161 aggcacgccg ggccgggatt ttccatcatc aggaggtcac cggttttgcc gcatcggtgg
     5221 cacttccact ccctgttcaa tggctggacc accaccagat ccggggcacg gcttgctttc
     5281 tccgccaggt gttggcgttt ccgctcggaa agctcttgcg atacccaatg ggtccggtac
     5341 agcgcctcga tcgttgggtc accgcttcgg ctgaaacgca acgtctgacg ccgcgggctg
     5401 cgcgcgatat aactcgtcgg gctcgcggac aagcctcttc gggtcgccca ggaccgaaac
     5461 agtttcatgg cctccgatat gcgcggcagg ttggtctgga caacacgttc gaggtagtcg
     5521 atctgacctc gccgccactg ttccaccttc ccggcatcca accacccgat cccgacgagc
     5581 acgtcgatcg agcttgcata gcctcgagca gccagcgccg cctccgcggc cttgacgaca
     5641 cgctcggcga gtgcgttccg gttcttgggg ttcatcgctg caaaaaacct cgttgcggtg
     5701 cagggcacgc cgaaccgtcg tccgcccaac gccgacacgc gctggcggcg cggtttcatt
     5761 ccctcaaagg gacaatccgc tcattccgtt caggtcgatc gctgacattg cggcatcagt
     5821 tcgctgaaac aaagcagcca atagacaaga aaacgaggtc ttcggcgaaa tatgctttcg
     5881 cgattccgcc ctacgcgtgc tgcgccgtat cggcgggatt cgcctcaggc cgccgtttcg
     5941 aagccgaacc cgtcatcgcc gagatgcttc tgcagttgct cgatccgccg ctgaatgagg
     6001 ggagcggtgt cgttcggacc gggaatatag acaggcttgc cgtcacgacc gaaccgaaag
     6061 accgcgtcgc ttgcgtccgc gctcacgtcg ccgaacatcc gctccaccgc tgcgaagtcc
     6121 cggtgcggcg cgaaaccgat cgattgcgac cacgcggcga gatcgcgcag gagcttgcgg
     6181 gcgtagctcg gatcgacgga aacgataggc gacccagcat ccgtcgcgtc catatacatc
     6241 tcgaaaactt cgctgtcgac cgattcaaac attacatcct tgatgccgag acagaacgtg
     6301 tcgatcagaa acgaacttaa cgcgacgtga tggggcgtgg cgccgcgcgc cagaaccagc
     6361 gtccccatgc caacgtcgaa tacggattcg gtgagaaggc agtgttgaat gggcgcttgg
     6421 gccgcccgga gcacacgggc gggcaggctt gcctccgata tctcggcccg tcgcttctgc
     6481 gccaccacct gtttccgccg ttgcgctttc ctgccgcgct tcagcgccat gttcgtcgcc
     6541 atggcccact cccaaagtga ttcggaattc ctgcattata caagtaaggt tccgcttggt
     6601 ggagtggcgg cgcgtcaaat attctgggga caaggctctg atcgaaactg caacggtggc
     6661 gcgcccgcct ggcgaatcac catgatctca acaaacttca gcccggtgcc atgagccttt
     6721 cagccgacgc cttatacaag acgtccgacg tggaattgct ggcggcgtat atcgaagccc
     6781 ggtgacaatt cgtggaaaag aggttcacgc gcgatacaca tcgggcgcgg ctggcgtgga
     6841 tcagggccaa aatgttcatc ggcagctcgg gcggggtcac cgagcgcaac atggccatcg
     6901 acgtctcgga agagattgcc cgcaagggtc aagaattgcg cgagatgacg cgcaacctcg
     6961 atctgctcaa ggtcgacgtc gacaccatcg cgatcgtcat tcgattccgc ggcgcatcgg
     7021 cgccgacagg cgtgcaaggc gaagacgcga ctgaaggcga tcccgaacgc ccttgagctg
     7081 cctcgtccaa ctgcgggcac cagtcggcga tttgcagatc aggttgcatc atcatgccta
     7141 ctctccccgc gctcacggag gcgggccgca aatttggcaa acctgtccga tcgtggtttg
     7201 acaattgtcc ccgtcacgtc tgcccccgcc aggtccacag ccgtaaggtc gacctcgctc
     7261 aacacagcgt agcgcaggtc ggtgccagcc aggcgcgccc cggcgagcgt cccccgatga
     7321 tggaagcccg gtacgcgcgt gctcggtcga gcttggcctc ggtgaggtcg cattcgtaca
     7381 gttgcgcctt ggtcaacgcg gcgcccgtaa ggtctgcacc ggcaagccgg cacccatgca
     7441 acgacgctgc cgccagatca gccccagaga gccctgcgcg cgcgagaacg cagttagtga
     7501 gcaccgcgtc gtagaacgtg gcccgatcag ccctcacgcc cgtcagatcg acctccgtca
     7561 gacgcgcgtc tgagaagtcg acggcttcca ggtgcgcccc ggccaagcct atgcgcgtca
     7621 acaccgcacc cgcgagaatc gcgcccggca tcgcgacgtc ggtgaggtcc acatcgtgaa
     7681 gacgaagctg cgacggcgtt gcccgcatac ctggtggccc ggaagcggtg gcgccaggct
     7741 gctgggcgta cttttcagcg acccactggc ggtgggcgtc aagagccgtc accagccagt
     7801 cgggcggtgg cgttggtgtt gactgattca tccgtgcgct ccgaaagcgg tattgacgaa
     7861 acagaatggg gtcaaagttc gacgcccatc ccagtcgagg ccgaggcgcg caacgtccgg
     7921 tccattcgac cgcctggaac atcatgtcac cggtcggagc gtccgcgcag ttcgtagcgc
     7981 actgcatcct gaaacgcgaa aaaattttgg aaactggaga tcagccatcc gtcatcggtt
     8041 gaatgaaagc ggccatgtag agacgaatgg gccacgatga taccattttg acggcgacct
     8101 gcgcgtgctt atccaaagcg ggacaccata gctcccgcgt ccaccaacca gtttccgtcg
     8161 cgaaacgcat tttacccgta tggaataagg gttttttcgc aacggagcct gcaacgcgaa
     8221 cgcggatggc tacttgcgcc gttcggcggc aatgcggtga agcttgctct gatatctcaa
     8281 taccgggcgg ctgatctcat ggcggacatc agctctttag gtcgtaccga gagtttgcaa
     8341 tcgatctcaa aaatttgcat ccactgatgt gaaccagctc acatcgactc cgcacgtttg
     8401 atccgacggt caagtggggc acctcaacgt aggagaggaa aatgcgcaca ctctttcttg
     8461 ccctcgcgac gtccgcgaca gtcttcgcaa ctggcgtaac cgaagtcgca gccggcgatt
     8521 atcgctattg catccaaggc gacgattttg gtgcgggcga ttgtagcttc gctagctacc
     8581 agcaatgcca ggccaccgcg tcaggtcgca cggcctactg cggagccaat cttcgtttcc
     8641 cgaatgtcgt cctaacagtt cgaactcggc tgcgtcatca ctaatggagt ggatcccggc
     8701 aggctgggag atgcacctga acgcgccatc gcggtgatcc aagacttcat ccgcactgat
     8761 tgaacgatat cggtcagcgt cgtagaggtg atcgcgtgcc gcttcacttc cgcttgtgtg
     8821 caggttttcg gatgccggcg tgacgaggct atccacgcgc tgcggcggcc ggcgtccgaa
     8881 aagcgccaat gatcgggata ggggggagcc tcgcgactcc acccctccca caccaccgta
     8941 catacgggtc cgtatacggc gg
//
LOCUS       DATNOI010000188         2806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1599472,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000188 DATNOI010000000
VERSION     DATNOI010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599472"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VN968_07250"
     CDS             complement(<1..549)
                     /locus_tag="VN968_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HXL29071.1"
                     /translation="MAIAIRAKQGLTRRHLLVRSASTLALAGLGSLARPYLSRAADRP
                     MIANGLASGDISTDSAVVWARADRPARMRVECSTVESFRTIIRVVSSDALPDHDFTSK
                     VLFENLPPGQDIFYRVRFEDISEPGISGEAQVGHFRTAPTDKGSISRSSISFVWSGDT
                     AGQGWGIDPARGGMRTYKTMLES"
     gene            complement(680..1123)
                     /locus_tag="VN968_07255"
     CDS             complement(680..1123)
                     /locus_tag="VN968_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29072.1"
                     /translation="MILKTILTAAICAVLLAGSSPSKADQYLPDEFLSLDLSRAVLSP
                     KPLGPTTEFTPVAIEARTDPGSEGVGTRAEPRANPRIAVSRSLKVAVPNVTVAHIRPQ
                     KPRGAVRAKLAQRHGNPLDAEARDTRIQVWPCQSGGICNWQRQND"
     gene            1425..1745
                     /locus_tag="VN968_07260"
     CDS             1425..1745
                     /locus_tag="VN968_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL29073.1"
                     /translation="MITAAQLRAARVLLGIDRRRLAELSRLSVPTIQRMEASETMVRG
                     NVDSLVKLIAAFNEAGIEMIDEGAVSSAQGRGVRLKTDSNSAKTVPGTRPENKAPSGT
                     RART"
     gene            1760..>2806
                     /locus_tag="VN968_07265"
     CDS             1760..>2806
                     /locus_tag="VN968_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003548932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXL29074.1"
                     /translation="MSAIVLQMSCVAGLLAISALAIALSRSRRATAAVYGATLAICVA
                     ALFGCLRWLVGDIANSSANASDVILPIGLPWLGAHFRLDALAAFFLVVVNLGGAAASL
                     YGLGYGHQDPAPHRVLPFFPAFLAGMNLVVLADDAFSYLLCWEFMSLVSWALVMAHHR
                     ETGNARAGYVYLVMASFGTLALLLAFGLLAGPAGDYGFAAIRAAQHTPYVAAMVLILM
                     LLGAGSKAGLVPLHVWLPLAHPAAPSHVSALMSGVMTKVAIYGFIRVVFDLLGQPTWS
                     ASVIVLFLGGITAIMGILYAMMEKDLKRLLAYSTIENVGIIFVSLGLALAFQANGLKL
                     PAALAFTAALFHVLN"
BASE COUNT          493 a          865 c          859 g          589 t
ORIGIN      
        1 gctttccagc atggtcttgt aggttctcat gccgccgcgc gcgggatcga tgccccagcc
       61 ctggcccgcg gtatcgcccg accagacaaa tgagatggaa gatcgcgata tcgagccctt
      121 gtctgtcggc gcggtccgga aatgcccgac ctgggcttcg cccgaaatcc cgggttcact
      181 gatgtcttca aaccggaccc gatagaaaat atcctgcccc ggcggaagat tttcgaatag
      241 cactttcgac gtgaagtcat gatccggcaa tgcatcggat gatacgacgc gaatgattgt
      301 cctgaagctc tcgacggtcg agcattccac ccgcatgcgg gccggccgat cggccctcgc
      361 ccacaccaca gcggagtcgg tggatatgtc gcctgaggcc agtccgttcg cgatcatcgg
      421 acgatccgcc gcccggctca gatacggcct ggcgagactt cccaacccgg ccagcgcgag
      481 cgttgatgct gaccgaacca gcagatgacg tctggttaaa ccctgcttcg cgcgtatcgc
      541 aatcgccatg gccggcaccc tcgtcgaatc gcgacggagg ttgcctgctc aacttgactc
      601 tgggccgacg gttttgcgac aggcggatga ctgaaagcga cccgcgcggg acggcgttgc
      661 gggcccggct acgccccgct caatcgttct gccgctgcca attgcagatg ccgcccgact
      721 ggcacggcca gacctgaatc cttgtatcgc gcgcttccgc atcgagcgga ttgccgtgac
      781 gttgcgcgag cttggctcgg accgcgccgc gcggtttctg cgggcggata tgcgcgaccg
      841 tgacgttggg aaccgcgacc ttgagactcc tggaaacagc gatcctggga ttggccctcg
      901 gttcggcgcg cgtgcccacg ccctcgcttc cgggatcggt tcgagcctcg atcgcaaccg
      961 gcgtgaattc ggtcgtaggc ccaagcggtt tcggcgaaag caccgccctg gaaaggtcga
     1021 ggctgaggaa ctcgtcggga aggtactgat ccgccttcga cggactgctc cccgccagca
     1081 gcacagcgca aatcgcggcc gtcaaaatcg ttttcagaat catttttgag gcccgctgaa
     1141 atgctgggga gaaccctatg taggacgcct gcagcgcaat tcaaacggaa gttggcgtca
     1201 aaccaatgtt gccaacaaca ttcgcgctcg tcgatgtcag cggccccgaa ggcatcttgt
     1261 gcgccatgat ggcttcggcg cgatgttcag tgcggcgacg gcgcggagga ccctgtgaag
     1321 aaaccattta gaatagatat attatatatc acttattgac actttaacat cattagttac
     1381 catgcacgaa tgatgtttgg ttaggggtcc ccgggggcag acttatgatc acagcggcac
     1441 aattgcgggc tgccagggtg cttcttggca tcgatcggcg tcggctcgcc gaactgtcga
     1501 ggctttccgt gcctaccatt cagcgcatgg aggccagcga aacgatggtc cggggcaatg
     1561 tcgattcgct ggtcaaattg atcgccgcgt tcaacgaggc cgggatcgag atgatcgacg
     1621 agggcgccgt cagttccgcg cagggccgcg gggtgcggct gaaaaccgat tcaaattcgg
     1681 cgaagacggt tccgggaact cgaccggaaa acaaggcacc gagcggaacc agggcgcgga
     1741 catgagttct gctgtcggta tctcggctat tgtcctgcaa atgtcgtgcg tcgcaggatt
     1801 gctggcgatc tcagcacttg cgatagccct gagccgctca cgccgcgcca cggcggcggt
     1861 ctacggcgcc acgttggcca tttgcgtggc cgccttgttc gggtgcctcc gctggctggt
     1921 cggcgatatc gcaaattcct cggcaaatgc ctcggacgtg atcctgccga tcggattgcc
     1981 gtggcttggc gcccatttcc ggctggatgc actggctgct tttttcctcg tcgtcgtcaa
     2041 tctgggcggc gccgcagcca gcctctatgg tctcggctac ggtcaccagg acccggcgcc
     2101 gcaccgcgtg cttccgttct tccccgcctt cctggccggc atgaatctcg tggtgctggc
     2161 ggacgatgcg ttttcctatt tgctttgctg ggaattcatg tcgctggttt cctgggcgct
     2221 ggtcatggcg caccatcgtg aaaccggcaa cgccagggcc ggatacgtct acctcgtgat
     2281 ggcaagcttc ggcacgctcg ccttgctgct ggccttcggc ctgttggcgg gaccggcggg
     2341 agattacgga tttgcggcca ttcgcgcggc gcaacatacg ccatatgtcg cggcgatggt
     2401 gttgatcctg atgctgctcg gagccggttc aaaggccggc ctcgtgccgt tgcacgtttg
     2461 gctgccgctc gctcatccgg cggcgccgag ccacgtgtcg gcgctgatga gcggcgtcat
     2521 gaccaaggtc gcgatctacg gcttcattcg cgtcgtcttc gatctcctgg gacagccgac
     2581 ctggtcggcg agcgtgatcg ttctctttct cggcggcatc accgccatca tgggaatcct
     2641 gtatgccatg atggaaaagg atctaaagcg cctgctcgcc tacagcacga ttgaaaacgt
     2701 cggcatcatc ttcgtcagcc tcggccttgc actggcgttc caggccaacg gcctgaagct
     2761 tccggcggca ctcgctttca ccgcggcgct gtttcacgtg ctcaac
//
LOCUS       DATNOI010000189        11793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1652623,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000189 DATNOI010000000
VERSION     DATNOI010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11793
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652623"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..942
                     /locus_tag="VN968_07270"
     CDS             160..942
                     /locus_tag="VN968_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL29075.1"
                     /translation="MPTLARAQSADLVLCDRLAADPSDPDKPADVKGTPDVATADVAT
                     AIKYCKIASASSPRALYQLGRAYAANRQLPEAMAAWRKAADKGSTSAMVELGVAYGTG
                     AGVARDEAQARKLFERAAEAGNPRGVTNLAALSGGGGGAPADPARARQLLAKAAEANA
                     EAQYQLGLMMANGTGGAQDDAGARNLFEKAAAQNHPGALERMGAFTQEGRGGPKDAGA
                     AKAYYERAAALGDEDAKKALKRMECPYAIKDKRGNFVTNLCF"
     gene            1030..1179
                     /locus_tag="VN968_07275"
     CDS             1030..1179
                     /locus_tag="VN968_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29076.1"
                     /translation="MIRKLQSGEYRLYSRKVNPKTGKRRNLGTFKSRAAAEKHERDVQ
                     YFKRH"
     gene            1605..2150
                     /gene="msrA"
                     /locus_tag="VN968_07280"
     CDS             1605..2150
                     /gene="msrA"
                     /locus_tag="VN968_07280"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXL29077.1"
                     /translation="MTVSTERAVLAGGCFWGVQDLLRRYPGVISTRVGYSGGEVPNAT
                     YRNHGNHAEAIEIIFDPKIIAYRKLLEFFFQIHDPSTLNRQGNDRGASYRSAIFYTSD
                     EQKRIAEDTIADVDASGLWPGKVVTEVAPVGPFWEAEPEHQDYLEKYPDGYTCHFIRP
                     NWTLPHRAETAQSGTRGATAA"
     gene            2147..3775
                     /locus_tag="VN968_07285"
     CDS             2147..3775
                     /locus_tag="VN968_07285"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012755690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HXL29078.1"
                     /translation="MTKGSDLLVAALENEGVQCIFAIPGEENLDVLESLRRSKVKLVL
                     TRHEQAAGFMAATYGRLTGRAGVCLTTLGPGALNLTTAAAYAHLGAMPMVMITGQKAI
                     RSSRQARFQIVDIVATMRPLTKMATQIVSAQSIPTLVRDAFRVAQQERPGPVHLELPE
                     DIAADAAAADIVPPHVVEIPIAPPAAIERAAAMIMAARRPLVMLGAAASRPHLAGPLS
                     AFVRRARIPFFNTQMGKGAVNAGSNLYIGTAALSERDWVHEAIDQADLIISIGHDTVE
                     KPPFLMGRNGPQVIHVGATPATVEQVYFPQAEIVGDVGASLAGLADRLEGKLPHASAL
                     LGLREGILAHLAEGSTEDRFPLTPQRIVHDVRKVIPPDGIVALDNGMYKIWFARNYRT
                     SVANTLLLDNALATMGAGLPSAIAASLINPDKRVLAVCGDGGFMMNSQELETAVRLKQ
                     NLVVLVLEDRAYGMIRWKQEVDGFSDFGLGFGNPDFVSYAKSYGAKGSRVGNAAEFAT
                     VLETAFLDGGVHLVVAPIDYSENMRVLVHELHSV"
     gene            complement(3916..6000)
                     /locus_tag="VN968_07290"
     CDS             complement(3916..6000)
                     /locus_tag="VN968_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HXL29079.1"
                     /translation="MKIGTLLTISIVSLSAVGGGLALYVAAAKYQTMDKISVAQRRLE
                     IVRAVGDIPRYMNPERGFATNIMFGPPAVDPALRAELNDKYRKQTDAAREKMNQIKPT
                     LAGTLEDGAAVASGIDALNVKFAVLRETIDKALDGPPEARRDGAKKAVADNVAFNTGV
                     TVLLDEQVRKMALLDGGAYRQASYANIAWTLRDVGGTNASLYKNLVAAKRVATDAEKL
                     DLSRSQGRNDQILLSLQELRGNPATPANVAAALTKMNEAYVERFGQEQKLVKEGAISG
                     KYEHDVETYYAESQRGLGAIIEVREAFYDNAEQLLSSAYSDARFSFIIALAGLAAVAG
                     ASVGLVMMVRRRVCKPIVDLTAMMSRLASGDVSDDISGSERDDEIGAMAAAVRVFKDN
                     MIKADRLAAEKDADNEVKMRRARLLDELTRAFETKVSELVGGLSSASSVMEETAQSMS
                     STASATNRQAGVVAAASEQTSANVQMVASATEELTSSVAEIGRQVAQSTEIAARAVES
                     ARRSGDTARSLADGAQKIGDVVTLIQTIAAQTNLLALNATIEAARAGDAGRGFAVVAS
                     EVKSLAGQTAKATTEISEQIAAIQTASDETVTAIRNVVDVIAEIDQIGVAIASAIDQQ
                     GTATKEISKNVQQAARGTQEVTSNISGVQRAADDTGSAANQVLGAAEQLSSQSRDLAG
                     QVNRFLSEVRAA"
     gene            complement(6445..6954)
                     /locus_tag="VN968_07295"
     CDS             complement(6445..6954)
                     /locus_tag="VN968_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012652479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29080.1"
                     /translation="MSSIYAASSTPATPDRALTVRAGLIGGLAGGIIIWIYEALVWVG
                     VQHLMPLAGIPRNATGLVFGKQVQEQLGTAAYFVGTGIHFLFALAWGVLFAYLWPIFR
                     RRGYEATLVALFYAIVIWIVMHAAIMVATTSHPDYFDPMVVIGGFMSHFFYTVPLALI
                     VKQRLAATG"
     gene            7131..7721
                     /locus_tag="VN968_07300"
     CDS             7131..7721
                     /locus_tag="VN968_07300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXL29081.1"
                     /translation="MTHRILVFYGSYRSDRLGIRLAQFVVEGLRGRGDEVELIDAKAV
                     GLPMLDRMYKEYPKGEAPERLEQLAGKIRDADGFVFITGEYNWGIQPGLKNLTDHFLE
                     EWFWRPAAIASYSAGRIAGARAAIAWHGTLSEMGMVVISSTIAAGPIAQTLSEKGEPI
                     GEGGKALAHGFPRFADDLAWWIEAAKAQRARKPPPY"
     gene            8115..8522
                     /locus_tag="VN968_07305"
     CDS             8115..8522
                     /locus_tag="VN968_07305"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXL29082.1"
                     /translation="MSEHLKEIPPAAVLLGREIISVDARSGEVQLRFLTDRQFANRHG
                     TIQGGMLAAMLDSATGNAVMANLPHDRTAVTTRLDTTFVKPAALGPLTATARLVKQDK
                     RSALAEAELVDSSGQIVARARAELRVRQRDTGI"
     gene            8640..9251
                     /locus_tag="VN968_07310"
     CDS             8640..9251
                     /locus_tag="VN968_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29083.1"
                     /translation="MVRKKAKRTTPTVCIVESLDFLQEDSLKEGEIISRTLRLSEKPA
                     HYTYLRSSDEMKAFVKEFGQSEHRYLHVSCHGNASTFFTTTGRMSAVDFAELLAPHVD
                     NRRVFLSACQAAQSEFAKSLLSNSECWSVLAPVDNIYFDDAAIFWSAFYHIMFKDNPD
                     SMHRSGIERAVKKCADLINAEFRLFFPEDGKVKSKTFGSTEER"
     gene            complement(9282..9716)
                     /locus_tag="VN968_07315"
     CDS             complement(9282..9716)
                     /locus_tag="VN968_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29084.1"
                     /translation="MRFRVRIFAHVLERLGLAMAGAACGLFVAALVGSSFALLTSQAF
                     LLLMMMGGAVGFYLGIDTPPLPFHPTKGPPSNGGAGGGIDSAEFLSAVGTFLATLSAF
                     ASVGVIVLRLDPHIAWTTAIMAGWVVGLAMQIIAGAITRTRR"
     gene            9842..10777
                     /locus_tag="VN968_07320"
     CDS             9842..10777
                     /locus_tag="VN968_07320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HXL29085.1"
                     /translation="MVFDHDLESSLDTVCKVAAGPVAGVFGPASLTWRIDREAAIFLG
                     AGRALLLQLAHPWVAAGIAEHSRTLADPIGRFHRTFNTVFTMVFGTLDSALAAARRLH
                     RRHGAVTGMLPSTAGRYAAGSAYRANEVSALRWVHATLVETALLTHDLVQPALDDGER
                     EQYWGEARLFAALFGIRQTDLPSNWTSFMAYNEAMWEADILTVSPAARTIAEQMLFGR
                     VAGLRVPKWYLALTAHLLPERLRGEFGLSFGEREARSMNLALARIRRFYPRLPMRLRT
                     VGPYQEALARLQGKKQPDLAVRSLNRLWIGRPMME"
     gene            11189..>11793
                     /locus_tag="VN968_07325"
     CDS             11189..>11793
                     /locus_tag="VN968_07325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL29086.1"
                     /translation="MPSRKPWSAKACETVFADVNRDTVTAAVKTSPRPDLAVAFVGDL
                     ASRDACDALLAHAESAVGRVTHFVHSASPPRREADHALAVSAETWEQMRAVNVDAGFH
                     LARELARKLIAAHEPGSFLFLTSLHASTPRNLPHYSTAKAALAMLVKELAKSFGRFGI
                     RVNALVPGAIAAGGFVADPALAKHIPVGRLGRGEDLAPMALA"
BASE COUNT         2173 a         3618 c         3779 g         2223 t
ORIGIN      
        1 ggcaccggtg ctgcgcgcct ccgggctcga gccttgaata tcagggcagt tcgacgctaa
       61 cctctctcgg tcaaaacgag cgtcatgatg atacggctcc aagcgttttc gttcgccgcg
      121 attggttgca tcgccgtccc ggcgatcatc attgtggcca tgccaacttt ggctcgcgcc
      181 caatcggccg atctcgtgct gtgcgatcgt ctcgccgccg acccgtccga tccggacaag
      241 ccggccgacg tcaaaggcac gcccgacgtc gcaaccgccg atgtcgcgac cgcgatcaaa
      301 tattgcaaga tagcttccgc ctcgtcgccg cgcgcgctgt accagcttgg ccgcgcttac
      361 gccgccaacc ggcaattgcc ggaagcgatg gctgcctggc gcaaggccgc cgacaaaggc
      421 tctacctcgg cgatggttga actcggcgtg gcctatggca ccggcgctgg cgtggccagg
      481 gacgaagccc aggcgcgaaa actcttcgag cgcgccgccg aagccggcaa tccgcgcggc
      541 gtcactaatc tggccgcact ctccggcggt ggtggcggcg cgccggcgga tccggcgcga
      601 gcaaggcaac tgctggcgaa agccgccgag gccaatgccg aagcgcagta tcagctcggc
      661 ttgatgatgg cgaatggcac cggcggggcg caggacgatg ccggcgcgcg gaacctgttc
      721 gaaaaggctg ccgcgcaaaa tcatcccggc gcactggagc ggatgggcgc gttcacccag
      781 gagggacgcg gcggaccaaa ggatgccggc gccgccaagg cctattacga aagggcggcg
      841 gcactcggcg atgaagacgc caaaaaggcg ctcaagcgca tggagtgccc ttacgcgatc
      901 aaggacaagc gcggaaattt cgtcaccaat ctttgtttct agagcgtcgt ccagggtcaa
      961 gccccatggg aacgcgagcg ggggtttcgc gattaatgca gggaaaacgc tgcaggaggt
     1021 ctttgtccca tgatccgaaa attgcaatcg ggcgaatatc ggctctattc ccgaaaggtg
     1081 aatccgaaaa ccggcaagcg ccgcaatctc ggtaccttca aatcacgcgc ggctgcggaa
     1141 aagcacgagc gggacgtgca gtatttcaag cggcattagc tgcttcagct gcttagcggg
     1201 gctcaacgcg accggaaaaa tcgttgagtc accctttgcg atcatcttga ccgtcgtcga
     1261 tgggcaggtt gctcgcttcc aaatgctgga agacagcttt gcagtctctc ggatggcccg
     1321 tggcggatgc ctacgccgcg ctgcgactgc tggctggtga tcccaggatc tctaggtgag
     1381 atggctggtc caccagccat cggcgctcgc gcgcagacaa agggatcgct gattagcccg
     1441 gttgcaggcg ccggccgcag ttgtttttct gaaagctcgc accacgtaag tgttggtgag
     1501 gaggcctcgg atatttgcat ttccgcatcg ttcggcctca ttgtagcctc ttcatggaag
     1561 ttcggagaac gacgccccgc gagggagcct tagggaggat gaacatgaca gtttcaacgg
     1621 aacgcgcagt ccttgccggt ggctgcttct ggggtgtgca ggatctgctg cggcgatatc
     1681 ccggcgtgat ttcaaccagg gtcggctaca gcggcggcga ggtgccgaac gcgacctatc
     1741 gcaatcacgg caatcatgcg gaagccatcg agatcatctt cgatcccaag atcatcgctt
     1801 accgaaaact gctggaattc tttttccaga tccacgatcc ctcgacgttg aaccgccagg
     1861 gcaacgacag gggcgcgagc taccggtcgg cgatcttcta taccagtgac gagcagaagc
     1921 ggatcgccga agataccatc gccgatgtcg atgcgtccgg cctctggccg ggcaaggtgg
     1981 tcaccgaagt ggctcccgtc ggacctttct gggaggccga gcccgagcat caggattatc
     2041 tggagaaata tcctgatggt tacacctgtc acttcatccg gccgaactgg acgctgccgc
     2101 accgggcgga gacagcgcaa tcaggcaccc gcggagccac ggcagcgtga caaagggctc
     2161 cgatctcctg gtggccgccc tcgagaacga gggcgtccaa tgcatcttcg ccattcctgg
     2221 cgaggaaaac ctcgacgtgc tggagtcgct gcggcgctcg aaggtcaagc tcgtgctgac
     2281 gcggcacgag caggccgccg gcttcatggc ggccacctac gggcgtctca ccgggcgcgc
     2341 cggcgtctgc ctgacgacgc tcggtcccgg tgcgctcaat ctgacgacgg ctgcggccta
     2401 cgcgcatctg ggcgcgatgc cgatggtgat gatcacggga cagaaggcga tccggagcag
     2461 ccgccaggcg cgcttccaga tcgtcgacat tgtcgcgacc atgcgacccc tgaccaagat
     2521 ggcaacgcag atcgtctcgg cgcagagtat tccaaccctg gttcgcgatg ctttccgggt
     2581 tgcgcagcag gagcggccgg gcccggtcca tctcgagctg cccgaggata tcgcggccga
     2641 cgcggccgcg gccgatatcg ttcccccgca cgtggtcgag attccgatcg cgcctccggc
     2701 tgcgatcgaa cgcgccgccg ccatgatcat ggccgccagg cggccgctcg tcatgctggg
     2761 cgcggcggcg agccgcccgc accttgccgg gccgttgtcg gcgttcgtgc gacgggccag
     2821 gattccgttc ttcaacaccc agatgggcaa gggcgccgtc aatgccggct ccaatctcta
     2881 tatcggcacc gccgcgctgt cggaacggga ttgggttcac gaggccatcg accaagccga
     2941 cctgatcatt tcgatcggcc acgataccgt cgagaagccg cctttcctga tgggccgcaa
     3001 cggaccgcag gtgatccatg tcggcgccac gccggcgacg gtggagcagg tctatttccc
     3061 gcaggccgag atcgtcggcg acgtcggcgc cagcctcgca ggcctcgccg accgcctgga
     3121 aggcaagctt ccccatgcct cggcattgct gggtttgcgg gagggcatcc ttgcacacct
     3181 cgcggagggt tcaacggagg atcggtttcc gttgacgccg cagcgcatcg tgcacgacgt
     3241 tcgaaaagtc attccgccgg acggcatcgt tgcgctcgac aacggcatgt acaagatctg
     3301 gttcgcccgc aactatcgca ccagcgttgc caacacgctg ctgctcgaca acgcgctggc
     3361 gacgatggga gcgggtcttc cttcggcgat cgcggcgtcg ctgatcaatc cggacaagcg
     3421 tgtgctcgcc gtctgcggcg acggcggctt catgatgaat tcccaggagc tcgagacggc
     3481 ggtgcggctc aaacaaaacc ttgtcgtgct cgtgctcgag gaccgcgcct acggcatgat
     3541 ccgatggaag caggaagtcg acgggttttc cgattttggt ctcggctttg gcaatcctga
     3601 tttcgttagc tatgcgaagt cctatggcgc caagggctcc cgcgtcggca acgccgccga
     3661 attcgcgacc gtgctggaaa ccgctttcct ggacggcggt gtgcatctgg tggtggcgcc
     3721 gatcgactac agtgaaaaca tgcgcgtcct cgtgcatgag ctgcactcgg tatgatggcg
     3781 tcgcaacccg cgtcttggtg gtcaatcctc gctacgagct tgaagaactc cagcgcgcgc
     3841 aaaatccctc gacatccccg gactgaaatt ccggcaagcg ttgccagatg gtttcggcgg
     3901 gccaatcccg ccggattacg cggccctgac ctccgagagg aagcggttga cctgcccggc
     3961 gagatctctc gattgcgaag ataattgttc cgcggcaccc aacacctgat tggcggctga
     4021 gccggtgtcg tcggccgcgc gctggacgcc ggatatgttc gaggtgacct cctgggtacc
     4081 gcgcgcggct tgctggacgt tcttggaaat ttccttggtc gcagttccct gttggtcgat
     4141 cgcggacgcg atcgctaccc cgatctgatc aatctcggcg atcacgtcga ccacgttgcg
     4201 gatcgccgtc accgtctcgt cgctcgcggt ctggatggcc gcgatctgct cggatatttc
     4261 ggtagtggcc ttcgcggtct ggccggcgag cgatttgact tcggatgcaa cgacggcaaa
     4321 accccgtccg gcgtcgccgg cacgcgccgc ctcgatggtt gcgttgagcg cgagcaggtt
     4381 ggtctgcgcg gcgatggtct ggatcagggt cacgacgtcg ccgatctttt gcgcgccgtc
     4441 ggccagcgag cgcgcggtat cgccgctgcg gcgggcgctc tcgacggcgc gggcggcgat
     4501 ttcggtggat tgcgcgacct ggcggccgat ctccgcgacc gaggatgtca actcctcggt
     4561 ggcactggcg accatctgca cgttggcgga ggtctgctcc gaggccgcgg cgacgacgcc
     4621 ggcctgacgg ttggtggccg acgcggtgga agacatcgac tgggcggttt cttccatcac
     4681 ggaagacgcc gacgacagcc cgccaaccag ttcggagacc ttggtctcga acgcgcgggt
     4741 gagttcgtcg agcaagcggg cgcgccgcat cttgacctcg ttgtccgcgt ctttctccgc
     4801 ggccaggcga tccgccttga tcatgttgtc cttgaagacc cgcaccgcgg ccgccatcgc
     4861 gccgatctcg tcgtcgcgct cgctacctga aatgtcgtcg gagacgtcgc cgctggcaag
     4921 acgcgacatc atggcggtga ggtcgacgat cggtttgcag acgcggcgac ggaccatcat
     4981 cacgaggccg acgctcgcgc ctgcgaccgc ggcgaggcct gcgagcgcga tgataaagct
     5041 gaagcgcgcg tcggaatagg ccgaactgag gagctgttca gcgttgtcgt agaaggcttc
     5101 gcgcacctct ataatcgcgc ctagaccgcg ttgcgactcc gcgtagtagg tctcgacgtc
     5161 gtgctcgtat ttgccgctga tggcgccttc cttgaccagc ttctgttcct ggccgaaacg
     5221 ctcgacatag gcttcgttca tcttggtgag cgccgccgcc acgttggctg gcgtcgcggg
     5281 attgccgcgc aactcctgca gcgacaacag gatctggtcg ttcctgccct gggagcggct
     5341 caaatccagc ttttcggcat cggtggccac gcgcttggcg gcgacgaggt ttttgtagag
     5401 gctggcgttg gtcccgccga cgtcgcgcag ggtccaggcg atgttggcgt aactcgcctg
     5461 ccgataggcg ccgccgtcga gcagcgccat ctttcggacc tgctcgtcga gcagcacggt
     5521 gacgccggta ttgaacgcca cgttgtcggc gacggccttc ttggcgccat cgcggcgggc
     5581 ctcgggcgga ccgtcgaggg ccttgtcgat ggtctcgcga agtactgcga attttacgtt
     5641 cagggcgtct atgccgctgg ccacggcggc tccatcctca agcgtgccgg ccagggtcgg
     5701 cttgatctga ttcatctttt cgcgggcggc gtcggtctgc ttgcggtatt tgtcgttgag
     5761 ttccgcgcgc agggccgggt ccaccgcggg aggcccgaac atgatgttgg tggcgaaccc
     5821 gcgctcgggg ttcatgtagc gcggaatgtc tcccacggcc cggacgatct ccagccggcg
     5881 ctgcgcgacg gagatcttgt ccatcgtctg gtatttcgcg gcggcgacat agagggcaag
     5941 accgccgccg acagcggaaa gcgagacgat tgagatcgtc agcagggtgc caattttcat
     6001 gagttggggg ccattcacga gggtggaggg agccgaattt cgatcatggt agttactccg
     6061 tattaatccc gagtttacgt tactgcacgc gaggggaggc aggtggttgc gtgggcgggg
     6121 gcatggaggg cgaattcgtc attccggggc gcgcgaatcg cgagccctga atccataacc
     6181 acgacgggga gtatggattc cgggcctgcg ccaagcggcg catccacgat gtgcgcttgc
     6241 acatcgggaa tgacagagtc tggattggtc gccgctgcgc tcctcacaaa gaccgtgata
     6301 ctccgcgaaa gcggagtatc cagtacgctg cggccttccg gctccatcac tggcgcctcg
     6361 gaacactgga tcgcccgctt tccgcaggca tcggcacatc gccgctgacc cgcaacgatg
     6421 ggagtggcga gcgggatgtt ggcgtcagcc ggttgcagca agccgctgtt tgacgatcaa
     6481 cgcgagcggc acggtgtaga agaaatgcga catgaagcca ccgatgacca ccatcgggtc
     6541 aaagtagtcg ggatggctgg tggtggcgac catgatcgcg gcatgcatca cgatccaaat
     6601 gactatcgcg tagaacaggg ccaccagcgt cgcctcgtag cctcggcgtc ggaagatcgg
     6661 ccacaggtat gcgaacagca cgccccaggc caacgcgaac aggaagtgga ttcccgtgcc
     6721 gacgaaatag gccgccgtcc caagctgctc ctgcacctgc ttgccgaaca ccaggcccgt
     6781 cgcgttgcgt ggaatgcccg ccaatggcat cagatgctgc accccgaccc agacaagggc
     6841 ttcgtagatc cagatgatta ttccccccgc cagtccgccg atcaaaccgg cgcggaccgt
     6901 caatgcccgg tctggtgtcg ccggcgtgga tgacgccgca tagatgctgc tcatgattgt
     6961 gtttgctccc cttgatttct tgtttaagag tcggccgtct cgcatatgcg agatatgcaa
     7021 cggatttcgc cgttcttcca agacgggtta tcttgcatcc ggtaaccggt cttcatcaag
     7081 ggaaatgggg cttgctaatc cagcctgcgg gctgatggtg tgtggatgcc atgacccatc
     7141 gtattctcgt gttctacggc tcgtatcgct ccgaccgcct gggcatccgc ctggcgcagt
     7201 tcgtcgtcga aggcttgcgc ggtcgcggcg acgaggtcga gctgatcgac gccaaagcgg
     7261 tcggcctgcc gatgctggac cggatgtaca aggaatatcc gaagggcgag gcgcccgaac
     7321 ggcttgagca acttgccggg aaaattcgcg acgccgacgg ttttgtcttc atcaccggcg
     7381 aatataattg gggcatccag cccggcctga agaatctcac cgatcatttt ctggaagaat
     7441 ggttctggcg tcccgccgcc atcgccagct attcggcagg gcgtattgcc ggcgcgcgcg
     7501 ccgccatcgc ctggcacggc acgctgtccg agatgggcat ggtggtgatt tccagcacga
     7561 tcgcggccgg gccgattgcg cagacactgt ctgaaaaggg cgagccgatc ggcgagggcg
     7621 gcaaggcgct cgcgcatggg tttccgagat ttgccgatga tctcgcctgg tggatcgagg
     7681 ccgccaaggc gcagcgggcg cgtaagccgc cgccgtattg agcagcgttc ggctaacgcg
     7741 cgccaagcct actcatggct gcaggagccg aaggtggccg gcgtagcttt ctaccgcgat
     7801 tggcttctcg ggggctgtca gcggactttc agcccatttc accgcggggt cgtcacaacg
     7861 gatgcattgg aggtcggtgc ggccggagag ggtgacaacc ggcaccatgc gttttccgca
     7921 tctcgggcag cgcgttaggt cattcatgag ctcctcccga ttttttctcc ataacaggga
     7981 aacacaactt ttgttccatc cgagtcgagc gttttgcgtg gttgcgatgc ggtgagtgag
     8041 gccccggatt tcgcttggct catccggacc acgcttgcta cgttccgcgc cgagcaacgg
     8101 gagtacggcg atcgatgtcg gaacatttga aggagatacc cccggctgcg gtcctgttag
     8161 ggcgggaaat catttccgtg gacgcccggt caggtgaagt gcaattgcgg tttctgaccg
     8221 accgtcaatt tgccaaccgt cacggcacca tccagggcgg catgcttgcc gcgatgctcg
     8281 actcggcgac cggcaacgcg gtcatggcca accttccgca tgatcgaacg gcggttacga
     8341 cgcgattgga cacgacattc gtcaagccag cggcgttagg accgctgacg gccaccgccc
     8401 gcctcgtcaa acaggataag cggtcggcat tggcagaggc cgagcttgtc gacagttccg
     8461 gccagattgt cgcccgtgcc cgcgcggagt tgcgcgtgcg acagcgcgat accggaattt
     8521 agcgcgcagg gcgcaatagc gagacgccga tcggcgcatg aattggcgga acacgctggg
     8581 cgattccgcc ctgtgggctg gcgccgggtt cgcgggtaaa tcgagggagg cagtagataa
     8641 tggtgcgcaa gaaggcaaaa cgaacgacac cgacggtctg catcgtcgaa agccttgatt
     8701 tccttcagga ggattctctc aaagaggggg aaatcatctc tcggaccttg cgcctgtcgg
     8761 aaaagcccgc gcactatacg tatttgcgtt catcggacga aatgaaggct ttcgtaaagg
     8821 agttcgggca gtcggaacat cgatatctcc acgtgtcgtg ccacggaaac gcaagcacat
     8881 ttttcactac cacgggccga atgtcggctg tggatttcgc tgaactgctc gcgccgcatg
     8941 tcgataaccg gcgggtattc ctgtcggctt gccaggcagc ccaaagcgag ttcgccaaat
     9001 cacttctgtc gaacagcgag tgctggtcgg tacttgcacc cgtagacaat atatatttcg
     9061 atgacgccgc gatattttgg tctgccttct atcatatcat gttcaaggat aatccggatt
     9121 cgatgcacag aagcggcatc gagcgggcgg tcaagaaatg tgcagatctg ataaatgccg
     9181 aattcaggct attttttccg gaagacggca aggtcaagtc gaagacgttc ggctcgacgg
     9241 aggaacgctg aagaggctcg ccgggcgaga gcggaagcgt ctcagcggcg ggtgcgcgta
     9301 atcgctccgg caatgatttg catggcgagg ccaacgaccc atcccgccat gatagctgtc
     9361 gtccaggcga tgtgcggatc gagacggagc acaatcacgc cgaccgaagc aaaagccgac
     9421 aacgtcgcga gaaacgtgcc taccgcgctc aaaaattccg cggaatcgat tccgcctccg
     9481 gcgccgccgt tagaaggcgg accttttgtt ggatgaaaag gcagcggagg ggtgtcgatg
     9541 ccgaggtaaa atccgacggc gccgcccatc atcatcaaga gcaggaaagc ctgggaggtc
     9601 agcagcgcga agctcgaccc caccagagcg gcaacaaaca ggccgcacgc agccccggcc
     9661 atcgcaagac ccaggcgttc cagaacgtgg gcaaatatgc gcacgcggaa tcgcatcgtg
     9721 gtaaccccgc agacgacgaa gtggttatgc taggacgttt tcctatggcg tgaaagggat
     9781 tacctgcagg gcaggcggcc aacccgttgg ccattgcacc ctgcgaaccg gggatatgcc
     9841 gttggtcttc gaccatgatc ttgaaagcag tctcgatacc gtctgcaagg tggcggccgg
     9901 accagttgcg ggagtcttcg gtccggcttc actgacctgg cggatcgacc gcgaggcggc
     9961 catcttcctc ggcgccggcc gcgctttgct gcttcaactg gcgcacccct gggtcgccgc
    10021 cgggattgcc gagcattccc ggactctcgc cgatccgatt ggccgtttcc accgaacgtt
    10081 caacacggtt ttcacgatgg tgttcggcac gctcgattct gcgctggccg cggcccgccg
    10141 gctgcatcgg cgccacggcg cggtgacggg aatgttgccg tcgacggcag gccgatacgc
    10201 ggcgggctct gcctaccgcg ccaatgaggt ctccgcgctg cggtgggttc acgccacgct
    10261 ggtggagacg gccttgctga cccacgatct cgtccagcct gcgctcgacg atggcgagcg
    10321 tgaacaatat tggggcgagg cccggctgtt cgcggccttg ttcgggattc ggcaaaccga
    10381 tttaccatcg aactggacct cgttcatggc ctacaacgag gcgatgtggg aagcggatat
    10441 tctcaccgtc agccccgccg cccgcaccat tgcggagcag atgctgtttg gccgcgtggc
    10501 aggattgcgc gtgccgaaat ggtatctggc cctgacggcc cacctgctgc cggaacggtt
    10561 gcgcggagaa ttcggcttga gcttcggcga acgtgaagcg cgcagcatga atctggcgct
    10621 ggcgcggatt cgacggttct atccccggct ccccatgcgc ctgcgaacgg tgggtcctta
    10681 tcaggaagcg ctggcgcgcc tgcaaggcaa aaagcaaccc gatctggccg tgcgttcgct
    10741 caaccggctc tggatcggcc ggccgatgat ggaataatgc ctcgtcatgc ccggacttga
    10801 tccgggcgca aggaaatcct ttcgaagaaa gatgggtcac cgggtcaagc ccggtgatga
    10861 cgggtgggtt ttgcggagca gggaaggagt gcttcgcgta catgccaact tgaacatttt
    10921 caaacagcca aagacgcgtc attgcgagcc aaggaaaatt gcaacgacgc ccgacgggca
    10981 aatcacccca tcgatccgat gacccattgt gcattggcgg gatacgctac accattgcgc
    11041 cccaagggct ggccacgcaa aagcgctgcc acgcaaaaac caactcaatt tcttttcgca
    11101 gggagtgatc acatgagcct gctggacaac ccattcgacc ccgcgcgcga ggcggctctg
    11161 gtgaccggcg cgggaaacgg catcggccgt gccatcgcgc aagccctggt cggcgaaggc
    11221 gtgcgaaacc gtgttcgccg atgtgaaccg ggatacggtc acggctgcgg taaaaacctc
    11281 gccgcggccg gacctggcgg ttgctttcgt cggcgacctc gccagccgcg acgcctgcga
    11341 tgcgctgctg gcgcacgccg agtccgccgt cgggcgggtg acgcacttcg ttcacagcgc
    11401 gtcaccgccg cggcgcgagg ccgatcacgc tttggcggtc agcgccgaga cctgggagca
    11461 gatgcgtgcg gtcaatgtcg acgccggctt tcatctcgcc cgcgaactgg cgcgaaaact
    11521 catcgcggcc catgagcccg gctcgttcct gttcctgacc tcgctgcacg ccagcacgcc
    11581 gcgcaacctg ccgcactact cgactgcaaa ggcagcgctc gccatgctgg tcaaggagct
    11641 tgcaaaaagc ttcggccgct ttggaatccg ggtcaacgcg ctggtgcccg gcgcgatcgc
    11701 ggcgggcggt ttcgtcgccg atccggcgct cgccaaacac atcccggttg gccgcctcgg
    11761 ccgaggcgaa gatctcgccc cgatggcgct ggc
//
LOCUS       DATNOI010000190         9247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1302068,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000190 DATNOI010000000
VERSION     DATNOI010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9247
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302068"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VN968_07330"
     CDS             <1..429
                     /locus_tag="VN968_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HXL29087.1"
                     /translation="RLMNMKPTGNGRRQGYAHVPMPRMTNTYMLAGERDPAEILASVK
                     NGIYAANFGGGQVDITSGKYVFQCTEAYKIENGKIGAPLKGAMLIGNGPTDLHRITMI
                     GNDLALDTGIGTCGKNGQGVPVGVGQPTLRMERITVGGTG"
     gene            429..1199
                     /gene="lepB"
                     /locus_tag="VN968_07335"
     CDS             429..1199
                     /gene="lepB"
                     /locus_tag="VN968_07335"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXL29088.1"
                     /translation="MSIGKESVASRAAGWGAQLLQLAAIVGIVFVAKGALAEPFYVPS
                     GSMEPTLLIGDALLASKYPYGYGTASLPIQINLPETGRVFAEPPKLGDVVVFRWPGDR
                     AQAWVKRVVGLPGDRIQMRQGQLFINDHAASLKPDGMGEAEDDNGAVEPAYRYIETLP
                     NGVAHAIFKLRDNGRLDNTPEVTVPPDRLFVMGDNRDNSADSRVPVREGGVGLLPMDN
                     LVGRADAVVGSWDLGMRSQPMWTWLSGFRGSRFFTAVR"
     gene            1332..1679
                     /locus_tag="VN968_07340"
     CDS             1332..1679
                     /locus_tag="VN968_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HXL29089.1"
                     /translation="MTFDDVRKIALAWPEVEDGTSYGTPALKVRKKLLVRLREDGDGL
                     VMPGVPPDERDMLVESQPKVFYFTDHYRDYPMVLIRLSKTKRAIVEPLLRRQWRTLAS
                     KAAIRAFDAGSTS"
     gene            1695..2267
                     /locus_tag="VN968_07345"
     CDS             1695..2267
                     /locus_tag="VN968_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXL29090.1"
                     /translation="MDKDDFIAAALRNPINEIIAEELSRLALPDAWIVAGCLVQTVWN
                     VQTARAVDHGIADYDIFYFDPDTSWQAEDAVIQKLQGRLAKLAVKVEVRNQARVHLWY
                     PQKLGLPYPALHCSTQGIDRFLTKNTQVGIRRTQSGHDVYAPNGFDDIAKMIVRPNPE
                     PNFSAANYEAKAERWKKLWPEITVLPAETK"
     gene            complement(2394..2948)
                     /locus_tag="VN968_07350"
     CDS             complement(2394..2948)
                     /locus_tag="VN968_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAN domain-containing protein"
                     /protein_id="HXL29091.1"
                     /translation="MWSGRLLRACLVTLAFSAVVAATRPAQAQANFDRPGGDYQSAPV
                     ESGDPADCALVCERDRRCRAWSFNYPTDIAGGAVCWLKSNVPARTQNNCCVSGVRGAG
                     VVEPRNGATETSIDRAGGDYRSFELKSGDGEEECKAACTGDNKCRAWTYARAGYVAKA
                     AHCFLKKEIKPPRRKAGFISGVVR"
     gene            complement(3171..4541)
                     /locus_tag="VN968_07355"
     CDS             complement(3171..4541)
                     /locus_tag="VN968_07355"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HXL29092.1"
                     /translation="MARDQIDMTPLQSRDELVAWLAAGVKPPSEFRIGTEHEKTPFTL
                     SGHHPVAYEGARGIGALLEGMQLLLGWEPIMECGNIIGLYDVTGGGAISLEPGGQFEL
                     SGAPVETVHQTQAELMAHLAQVREIAAPLGIGFLGLGMTPSWSRAQIPMMPKGRYKIM
                     TNYMPKVGQYGLDMMYRTCTVQTNLDFSSEADMVKKLRVSVALQPVAAALFANSPFTE
                     GEPNGFLSFRSEIWRETDSARSGMIPWAFEGGMGFERWVDYALDVPMYFVKRGDAYID
                     VAGSSFRDFFAGKNPAVPGERPTLSDWANHLSTIFPEVRLKRYLEMRGADGVPWGRLP
                     ALPAFWVGLLYDGTSLDAAWDIVKHWNAGERQALRDDVPRLGFKARIRDRYLFEIARE
                     CVALSHSGLRRRGRIDHLGRDESRHLEPLERTIDSGRTPAEEMLEKFKGPWNGSVEPA
                     YDEYAF"
     gene            complement(4638..5126)
                     /locus_tag="VN968_07360"
     CDS             complement(4638..5126)
                     /locus_tag="VN968_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HXL29093.1"
                     /translation="MKRKPSTEATAAWIRLMRVQGRVLDAVEQDLKKAGFPPLAWYDA
                     LLELSRSPSGAVRPVELEKQMLIPQYSTSRLIDRLVDEGLAARRECKIDKRGQFVEIT
                     EAGRELQKKMWSAYSAAIEKHVGSKLSDADAARLCALLDRLGCSCDEAQALAARDGAP
                     AR"
     gene            5426..6040
                     /gene="gstA"
                     /locus_tag="VN968_07365"
     CDS             5426..6040
                     /gene="gstA"
                     /locus_tag="VN968_07365"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HXL29094.1"
                     /translation="MKLYYSPGACSLSPHIALLEAGLPYDLVKVDLKAKQLENGDDYL
                     KVNPKGQVPALGLDSGELVTEGPVIVQMIADKVAAKNLAPARDSAERYKLQEWLNFIT
                     TEVHKNFGPLFSPVLADDAKAFFKDRVMGKFKYIDSQLPGHDYLMGKQFTVADGYLFT
                     MLCWADRMKFDLSALPDLLAYKARVGARPMVQEALTKEGLMKAA"
     gene            complement(6211..8427)
                     /locus_tag="VN968_07370"
     CDS             complement(6211..8427)
                     /locus_tag="VN968_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXL29095.1"
                     /translation="MAFKNFKVETDADGIALVTWDIPGRSMNVLDETTITELEAIVKQ
                     TSADAAVKGVVVTSGKEAFSAGADLSMLEGMNRRYTDLLKAKGEVAANQMLFDESRKS
                     SLVFRAIETSGKPWAAAINGLALGGAFELTLSCHYRVAAENPKTRLGLPEAKVGLFPG
                     AGGTQRVPRIVSPQDAMPLLLKGETVNLEKAKALKLIDAVVPAGDLIKAAKDWIKGGG
                     KAVAPWDEKGFKLPGGPVFSKAGMMMFPAGNAIYRRETYDNYPAQRAIMSCVYEGLLL
                     PIDAALRVESRYFAQVLRSSEAAAMIRSLFLSMQELNKGARRPQNVPPTKVKKLAVIG
                     AGFMGASVGYVSARAGIEVVLIDRDQESADKGKGHAKAVIDDLIKKGRAKEADRDAIL
                     SRISATADYNVLKDCDLIIEAVFEDRKVKAETYAKAQPLLKPDAIFASNTSTLPINSL
                     AEEFKDQGKFIGIHFFSPVEKMMLVEIIVGKNTGDIALATALDYVRAIGKTPIVVNDS
                     RGFFANRCVLRFTAEGLEMLMEGVPPAMIENTAKMAGMPVGPLSLSDEVALDLVLKIM
                     KATEADLGANAIDQTQKKLLVEMVEKQGRFGRKNGKGFYDYPEKGKGQKSLWSELSGL
                     QPKHLDPDTLDIEELKQRFLVVQAVEAARTVEDHVITDMREADVGSILGFGFAPFTGG
                     TLSYIDFMGTKKFVELCQKLEAKYGSRFTPPKLLIEMAAKGETFYGRFPPKKLAAA"
     gene            complement(8562..>9247)
                     /locus_tag="VN968_07375"
     CDS             complement(8562..>9247)
                     /locus_tag="VN968_07375"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXL29096.1"
                     /translation="RAAKAWDEGRFNNSVIPVKDVNGLTILAKDEHMRPTTTMQSLGQ
                     LQASFAAVGVMGGFDAVAIQSHPEIERINYVHHAGNSSGIVDGAGAVLLGSKEAGAKH
                     GLKPRAKIRAFTNIGSEPAMMLTGPVDVTEKLFERSGMKKSDIDLFELNEAFASVVLR
                     YIQAFDIDNAKINVNGGAIALGHPLGATGAMILGTVLDELERTNKSTALITLCIGGGM
                     GTATIIERV"
BASE COUNT         1748 a         2963 c         2766 g         1770 t
ORIGIN      
        1 cggctgatga acatgaagcc gaccggcaac ggccgccgcc agggctatgc ccatgtgccg
       61 atgccgcgca tgaccaacac ctatatgctc gcaggcgagc gcgatccggc ggaaatcctg
      121 gcctcggtca agaacggcat ctatgccgcg aatttcggcg gcggccaggt cgacatcacc
      181 tcaggtaaat atgtcttcca gtgtaccgag gcctacaaga tcgagaacgg caagatcggc
      241 gcgccgttga agggcgccat gctgatcggc aacgggccga ccgacctgca ccgcatcacc
      301 atgatcggca acgatctcgc gctggatacc ggcatcggca cctgcggcaa gaacggccag
      361 ggtgtgccgg tcggcgtcgg ccagccgacg ctgcggatgg agcggattac cgtgggagga
      421 accggctgat gagcatcgga aaagaatcag tagcctcgag ggcggcgggc tggggcgcgc
      481 aacttctcca gctcgcggcg atcgtgggta ttgttttcgt tgccaagggc gcgctcgccg
      541 agccgttcta tgtgccgtcg ggctccatgg agccaacgct gctgatcggc gatgcgctgc
      601 tggcgtcgaa atatccctat ggctacggca ccgcctcgct tccgatccag atcaacctgc
      661 ccgagaccgg gcgggtgttc gccgaaccgc cgaagctcgg cgatgtcgtg gtgttccgct
      721 ggccgggcga tcgcgcgcag gcctgggtca agcgtgtcgt cggcctgccc ggcgatcgca
      781 tccagatgcg gcagggccag ctgttcatca acgaccatgc cgcgtcgctg aaacccgacg
      841 ggatgggcga agccgaggac gacaacggcg cggtcgaacc ggcctatcgc tacatcgaga
      901 ccttgccgaa cggcgtcgcg cacgcgatct tcaagcttcg cgacaatggc cggctcgaca
      961 acacccccga agtgacggtg ccgccggacc ggctgttcgt gatgggcgac aaccgcgaca
     1021 actccgccga cagccgcgtc ccggtgcgcg aaggcggggt cgggctattg ccgatggaca
     1081 atctggtcgg ccgcgccgac gccgtcgtcg gctcctggga tctcggcatg cgcagccagc
     1141 cgatgtggac ctggctgtcg ggttttcgcg gttcgcggtt cttcaccgcg gtgcgctaga
     1201 gttgcgttcg acaagatcga aacgcaactc tggatttttg atttgacgcg ttttcttgac
     1261 gcgaaccggc cgccaccccc ggatcaagtc cgagggcatg cttcgcttga aagcactctg
     1321 gacgcgccac catgaccttc gacgacgtca gaaagatcgc actggcgtgg ccggaggtcg
     1381 aggacggcac ttcctatggc acgccggcgc tcaaggtgcg caagaagttg ctggtgcggc
     1441 tgagggaaga cggcgacggc ctggtaatgc cgggcgttcc gcccgatgag cgcgacatgc
     1501 tggtggagag ccagccgaag gtgttttact tcaccgacca ctacagagac tatccgatgg
     1561 tgctgatccg gctgtcgaag acaaagcgcg ccatcgtcga gccgctgctg cgccggcaat
     1621 ggcggacgct ggcttcaaaa gcggcgatca gggcattcga cgccggttcg acgtcatgac
     1681 cggcatgcgc gcccatggac aaagacgact tcatcgccgc cgcgctccgc aatcccatca
     1741 atgaaataat tgccgaagaa ttatcccggc tggcgctgcc ggatgcgtgg attgtcgccg
     1801 gatgcctggt gcagacggtg tggaacgtac agaccgcgcg cgcggtcgat catggtatcg
     1861 ccgactacga tattttctat ttcgatcccg acacgtcctg gcaggccgaa gacgccgtga
     1921 tccagaaatt gcaaggccgg ctggcaaaac tcgccgtcaa ggtcgaggtg cgcaatcagg
     1981 cccgcgttca tctctggtat ccgcaaaagc ttggcctgcc ctaccccgcg ctgcattgtt
     2041 cgacccaagg catcgatcgt ttcctgacga aaaataccca ggtcggaatc cgccgcacgc
     2101 aatcaggcca cgacgtctac gcacccaacg gctttgacga tatcgccaaa atgatcgtcc
     2161 ggcccaatcc cgagccgaat ttctcggcgg cgaattacga ggcaaaggcg gagcgctgga
     2221 agaagctgtg gccggaaatc acggtgctgc cggcggagac aaaatagtcc gatgtcgtta
     2281 tgtgacgacc aatcagacac ggtcattccg gggcgatgca aagcatcgaa cccggaatct
     2341 cgagatttcg ggtctggtgc taacgcacca tcccggaatg acggtgcaaa tccctaccgc
     2401 accacgcccg agatgaaccc ggccttgcgc cggggcggct tgatttcctt tttcaggaag
     2461 cagtgcgcgg cttttgcaac atatcccgcc cgcgcatagg tccaggcccg gcacttgttg
     2521 tcgccggtgc aggccgcctt gcactcttct tccccgtcgc cgcttttcag ttcgaaactg
     2581 cgataatcgc cgccggcgcg gtcgatcgag gtttcggtcg cgccgttgcg cggttcgacc
     2641 acgccggcgc cgcgcacgcc cgagacgcag caattgttct gggtgcgcgc cggcacgttg
     2701 ctcttgagcc agcagaccgc gccgccggcg atatcggtcg ggtagttgaa gctccaggcc
     2761 cggcagcgtc ggtcgcgctc gcataccagc gcacaatccg ccggatcgcc cgactccacc
     2821 ggcgcactct gataatcgcc gcccggccga tcgaaattgg cctgagcttg cgccggtcgc
     2881 gtggcggcga cgaccgccga aaaagcgagc gtcaccaaac acgccctgag caggcgccca
     2941 ctccacatct gcgattgctt tcggtcagcg catgatccgc caacatgtaa gcggtttggc
     3001 gaaaagatca tgcgctcttt caatagaatt agagcgcgat cggacgcaaa acagggtccc
     3061 acttttgctg atcgcgctct cgttttgact tacccgttcg cggggcgcca tttgatcgcc
     3121 atcaacctgt acgctggatg aacgctacaa aaatctgcca acatgctgac ctagaatgca
     3181 tattcgtcat aggccggttc aacggagccg ttccaggggc ctttgaattt ctccagcatc
     3241 tcttcggcgg gggtgcgccc tgagtcgatc gtccgctcca gcggctcgag atgccggctc
     3301 tcgtcgcggc cgaggtgatc gatgcggccg cgccgccgca gccccgaatg cgacagcgcc
     3361 acgcactccc tggcgatttc gaacaaatag cgatccctga tcctcgcctt gaagcctagt
     3421 cgcggcacgt cgtcacgcaa cgcctgacgc tccccggcat tccagtgctt gacgatgtcc
     3481 cacgcggcgt cgaggctagt accgtcatag agcaatccga cccagaatgc cggcagcgcc
     3541 ggcagccgtc cccacggcac gccgtcggcg ccacgcattt ccagatagcg cttcagccgc
     3601 acttcgggaa agatcgtcga aagatggttg gcccaatccg acagcgtcgg acgctcgccg
     3661 ggaactgccg gattcttacc ggcgaagaaa tcgcggaacg acgatccggc gacgtcgata
     3721 taggcgtcgc cgcgcttgac gaaatacatc ggcacatcga gcgcataatc gacccagcgc
     3781 tcgaacccca tgccaccctc gaacgcccat ggaatcatcc cggatcgcgc gctgtcggtc
     3841 tcgcgccaga tctcggagcg gaacgacagg aagccgttag gttcgccttc ggtgaacggc
     3901 gaattggcga acaaagcggc ggccaccggc tgcagcgcaa ccgatacccg cagcttcttg
     3961 accatgtcgg cttccgacga aaagtcgaga ttggtctgca ccgtgcaggt ccggtacatc
     4021 atgtcgagcc cgtattggcc gaccttcggc atgtagttgg tcatgatctt gtaacggccc
     4081 ttaggcatca tcgggatctg cgcccgcgac catgacggcg tcatgccgag cccgagaaac
     4141 ccgattccca agggggcagc gatctcgcgc acctgcgcca gatgcgccat cagttcggct
     4201 tgggtttgat gcacggtctc gaccggcgcg cccgacagtt cgaattgtcc gccgggctcc
     4261 agcgaaatcg cgccgccgcc ggtgacgtca tacaggccga tgatgttgcc gcactccatg
     4321 atcggctccc agccgagcag gagttgcatg ccctccagca gcgcgccgat gccgcgcgcg
     4381 ccttcgtagg cgacgggatg atggccggaa agcgtgaacg gcgttttttc gtgttcggtg
     4441 ccgatccgga attcggaggg cggcttcacg cccgccgcca gccacgcaac caattcgtca
     4501 cgcgactgca gcggcgtcat atcgatctgg tcacgcgcca taaaatttcc ggatgtgggg
     4561 cgtttcgacc gaacgcgcac ggtgtcgggg aaggttacgg acagcattgt ccgcaaacac
     4621 ggaaagtaaa gctaatatca tcgcgcgggc gcgccgtctc ttgcggcgag cgcctgagcc
     4681 tcatcgcacg agcagcccag ccggtccagc agcgcgcaaa gccttgctgc gtccgcatcg
     4741 gacaatttcg agccaacatg cttttcgatg gcggccgaat aggcgctcca catcttcttc
     4801 tgcaattcgc gccctgcctc ggtaatctcg acaaactggc cgcgcttgtc gatcttgcac
     4861 tcgcggcgcg ccgccagccc ctcgtccacc agccggtcga tcagccgcga ggtcgagtat
     4921 tgcggaatca gcatctgctt ttccagctcc acagggcgca cggcgcccga aggcgaacgc
     4981 gacagctcga gcagcgcgtc gtaccaggcc agcggcggga aaccggcctt tttcagatcc
     5041 tgctcgacgg cgtcgagtac ccggccctga acccgcatca ggcggatcca ggctgccgtc
     5101 gcctcggtcg atggtttgcg tttcatggcc tggtgccctg tttccgaaat tcgtaatata
     5161 ttgcggcacc ctctgatacg aagggcacga ccaaatccaa gaccaactct gtgaccaacg
     5221 ccatgactcc cgtgccgcgg ttcgatgtgg agtccctaaa gcctgcggta accggtgcag
     5281 gaacttcaaa tcggcagcac ccatccataa tgtgacgaaa cagtgtaccc cactaaatgc
     5341 atttgcatca atcttgacta attcatgcag atgcatttac tcgtccggtc tcccatagcc
     5401 cataatccaa aggaaggaaa ttccgatgaa actgtattat tcgcccggcg cctgttcgct
     5461 ttccccgcac attgcgcttc tggaggcagg gctgccctac gatctggtca aggtcgacct
     5521 gaaagccaag cagctcgaga atggtgacga ttacctgaag gtgaacccaa aaggtcaggt
     5581 cccggcgctc ggcctcgaca gcggcgaact ggtgaccgaa ggtccggtca tcgtgcagat
     5641 gatcgccgac aaggtcgcgg ccaagaacct cgcgcccgcc cgcgacagcg ccgagcgcta
     5701 caagctgcag gagtggctca acttcatcac cacggaagtg cacaagaatt ttgggccgct
     5761 gttcagcccg gtgctggccg acgacgccaa ggcgttcttc aaggaccggg tgatgggcaa
     5821 gttcaaatat atcgatagcc agcttcccgg ccacgactac ctgatgggca agcagttcac
     5881 cgtcgccgac ggctatctgt tcacgatgtt gtgctgggcc gatcggatga agttcgatct
     5941 ttccgcgctg ccggaccttc tggcctacaa agcccgcgtc ggcgcccgcc cgatggtcca
     6001 ggaagccttg accaaggaag gcctgatgaa ggcggcgtga gctttcgcgt ccgcgcgaag
     6061 caccgcaacc gcatcgtcgt cccggcgaag gccgggatcc ataaccagag gccttcgtgg
     6121 tgaaagaagg cgtctactgc ggtgcccttc agatgggaca cagcgtatgg gtcccggcgt
     6181 gcgccgggac gacgatcttc ttacccttcg ttacgccgcg gccagcttct tcggcgggaa
     6241 gcggccatag aacgtctcgc ccttggccgc catctcgatc aacagctttg gcggggtgaa
     6301 gcgcgagccg tatttggctt ccagcttctg gcacagttcg acgaactttt tcgtgcccat
     6361 gaagtcgata taggacagcg tgccgccggt gaacggcgca aagccgaagc cgaggatcga
     6421 gccaacatcg gcctcgcgca tatcggtgat aacgtgatct tccaccgtgc gcgcggcttc
     6481 caccgcctgc accaccagaa atcgctgctt caactcctcg atgtcgagcg tatcgggatc
     6541 gagatgtttc ggctgcaacc ctgaaagctc cgaccacagg cttttctggc ccttgccctt
     6601 ctcgggataa tcgtaaaatc ccttgccgtt cttgcggccg aagcggccct gcttttcgac
     6661 catctcgacc aagagcttct tctgggtctg gtcgatcgcg ttggcgccga ggtcggcctc
     6721 ggtcgccttc atgatcttga gcacgagatc gagcgcgacc tcgtcggaca gcgacagcgg
     6781 cccgaccggc atgccggcca ttttcgcggt gttctcgatc atcgccggcg gcacgccttc
     6841 catcagcatt tccaggcctt cggcggtgaa gcgcagcacg cagcggttgg caaagaagcc
     6901 gcgggaatca ttgaccacga tcggcgtctt gccgatcgcg cgcacgtaat ccagcgcggt
     6961 cgcgagtgcg atgtcgccgg tgttcttgcc gacgatgatc tccaccagca tcattttctc
     7021 gaccggcgaa aagaagtgaa tgccgatgaa cttgccctga tccttgaact cttccgccaa
     7081 cgaattgatc ggcaaggtcg aggtgttgga ggcgaagatc gcgtccggct tcaacagcgg
     7141 ctgcgccttg gcataagttt ccgccttgac cttgcggtcc tcgaacaccg cttcgatgat
     7201 gagatcgcag tccttcaata cattgtagtc ggcggtcgca ctgatgcgcg acaggatggc
     7261 gtcgcgatcg gcttccttgg cgcggccctt cttgatcaaa tcgtcgatca cggccttggc
     7321 gtggcccttg cccttgtcgg cgctctcctg atcgcggtcg atcagcacca cctcgatgcc
     7381 ggcacgcgcc gagacgtaac cgacgctggc gcccatgaag ccggcgccga tgacggcgag
     7441 ttttttcact ttggtcggcg gcacattttg cggccgccgc gcgcccttgt tcaattcctg
     7501 catcgacagg aacaggctgc ggatcatcgc ggccgcttcg ctggagcgca gcacttgcgc
     7561 gaagtaacgc gattcgacgc gcagcgccgc gtcgatcggc aacagcaagc cttcgtagac
     7621 gcaactcatg atggcgcgct gggccggata gttatcatag gtctcgcggc ggtagatcgc
     7681 gttgccggcc ggaaacatca tcatgccggc tttggaaaaa accgggccgc ccggcaactt
     7741 gaaacccttc tcatcccagg gtgcaacggc cttgccgccg cccttgatcc agtctttcgc
     7801 cgccttgatc agatcgccgg ccggaacgac agcgtcgatc agcttcagcg ccttggcttt
     7861 ttcgagattg accgtctcgc ccttcaacaa cagcggcatc gcgtcctgtg gcgacacgat
     7921 gcgcggcacc cgctgcgtgc cgccggcgcc gggaaacaag ccgactttcg cttcgggcaa
     7981 accgagccgg gtcttcggat tttccgcggc gacgcggtag tggcacgaca atgtcagttc
     8041 gaacgccccg ccgagcgcga gcccgttgat cgcggcagcc cacggcttgc cgctcgtctc
     8101 gatcgcccga aacaccagcg aggatttacg gctctcgtcg aacagcatct ggttggccgc
     8161 gacctcgccc ttggctttca gcaggtcggt atatcgacga ttcatgccct ccaacatcga
     8221 caagtccgcg cccgcggaga acgcctcctt gcccgaggtg acgacgacgc ccttcaccgc
     8281 ggcgtcggcg gaagtctgct tcacgattgc ttcgagctcg gtgatcgtgg tctcgtcgag
     8341 cacgttcatc gaacggcccg gaatgtccca ggtcaccagc gcaatgccgt cggcgtcggt
     8401 ttcgactttg aaattcttga aggccattgt gtttgctccc gattcccgtt tccgtcatcc
     8461 tgaggtggcc tggccctttg cgaggccctc gaaggatgac ccgatgaaat tatccatcct
     8521 tcgaggctcg ggcgttgccc gagcacctca ggatgacgcc gtcagactct ttcaataatg
     8581 gtcgcggtgc ccatgccgcc gccgatgcac agcgtgatca gcgcggtcga tttgttggtg
     8641 cgctcgagtt cgtcgagcac ggtgccgaga atcatcgctc cggtagcgcc gagcggatgg
     8701 ccgagcgcga tcgcgccgcc attgacgttg atcttggcgt tgtcgatgtc gaaggcctgg
     8761 atgtagcgca gcaccaccga ggcgaacgcc tcgttcagct cgaacagatc gatgtcggat
     8821 tttttcatgc ccgagcgctc gaacagcttt tcggtgacat cgaccggacc ggtcagcatc
     8881 atcgccggct ccgagccgat attggtgaag gcgcggatct ttgcacgcgg ttttaaaccg
     8941 tgtttcgcgc cggcctcctt gctgccgagc aataccgcgc cggcgccgtc gacgatcccg
     9001 gaggaattgc cggcgtggtg cacgtagttg atgcgttcga tttccggatg cgactggatc
     9061 gccaccgcat cgaagccgcc catcaccccg accgccgcga acgacgcctg caactggccg
     9121 agcgactgca tcgtcgtggt gggccgcatg tgctcgtcct tggcgagaat ggtgaggcca
     9181 ttgacgtcct tcaccgggat caccgaattg ttgaagcggc cctcgtccca cgccttggcg
     9241 gcgcgtt
//
LOCUS       DATNOI010000191         2104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1892863,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000191 DATNOI010000000
VERSION     DATNOI010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892863"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VN968_07380"
     CDS             complement(<1..220)
                     /locus_tag="VN968_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29097.1"
                     /translation="MMTAARAMRFAAGRVIIVIAMALLAAADDRIAPMPGVFPDHPAP
                     VPANSFAEYAPEPNPTPGKKDVLRCALND"
     gene            complement(217..>2104)
                     /locus_tag="VN968_07385"
     CDS             complement(217..>2104)
                     /locus_tag="VN968_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXL29098.1"
                     /translation="EHGMRPYEAALKGAREIGFTIISITFSLIAVFIPVLLMGGIVGR
                     VFREFAVTVSVAIIVSGFVSLTLTPMLCARVLKAHDETRKPNIVLRAFERVFDSWLRG
                     YEWALDWVLAKKAVMLLVTLATLGGTVYLYMIVPKGFFPQEDTGFLIGVTEAATDTSF
                     EAMKVRQQALVDVLKSDPAIDYINSTVGSGGPNPTANYGRLFIALKPQKERDGAAAVI
                     GRLRQKATQIPGMQAFFQSIQNLSVGGRISKSQYQYVLQSGDTESLYRIAPEMRDKIA
                     KIPGLLDVTTDLYIKNPQMTVDIDREKAAVYGVTVDQVRNQLYNAYGGRQVGTIYMPS
                     NDYQIILEAQPEFRVDPSDLSKLYMKTANNQTIPLDAVAKLVPTVGPLQINHQGQQPA
                     VTISFNLAPNYSLGYAVDQITAIEQSSNLPATIATGFSGTAQVFQDSLRGQGVLILAA
                     VFAAFVILGILYESFIHPITIISGLPSAGIGAILTLMLFNMELSVIAMIGIVMLVGIV
                     KKNAIMMVDFAIERRRVGLSAEHAIREAALLRFRPIMMTTFAAIFGTLPIAMGAGAGA
                     ELRQPLGIAVVGGLCVSQLLTLFITPVIYIYLDRIDRRLKRRLEPQAGEAEGAERPQV
                     VAAE"
BASE COUNT          422 a          642 c          653 g          387 t
ORIGIN      
        1 cgtcattgag cgcgcaccgt agaacgtcct ttttgccagg cgttgggttt ggctctggcg
       61 catattccgc aaagctgttt gccggcacag gagcgggatg gtcggggaac acaccgggca
      121 ttggcgcgat acgatcatcc gccgcggcga gtagtgccat ggcgatcacg atgatgacac
      181 gacccgcagc gaaccgcatc gcccttgccg ccgtcatcac tctgccgcga cgacttgcgg
      241 ccgctcggcg ccctcggcct cgcctgcttg cggttcgaga cgacgcttca ggcggcgatc
      301 gatccggtcg agatagatat agatcaccgg cgtgatgaac agcgtcagga gctgcgacac
      361 gcagaggccg ccgaccacgg cgatgccaag cggctgacgc agttccgcgc cggcgcccgc
      421 gcccatcgca atcggcaacg tgccgaaaat cgcggcgaag gtcgtcatca tgatggggcg
      481 gaagcgcagc agtgccgcct cgcgaatggc gtgctcggcg ctcaagccga cgcggcgccg
      541 ctcgatcgca aaatccacca tcatgatggc gttcttcttg acgatgccga ccagcatcac
      601 gatgccgatc atcgcgatca ccgacagttc catgttgaac agcatcagcg tgaggatggc
      661 gccgatgccc gccgagggca ggccggaaat gatggtgatc ggatgaatga agctctcgta
      721 gagaatgccg aggatcacga acgccgcaaa gacagcggcc aggatcagca caccctgccc
      781 gcgcaaggaa tcctggaaca cctgcgccgt gccggagaag ccggtggcga tggtggccgg
      841 cagattcgag ctttgctcga tcgcggtgat ctggtccacc gcatagccaa gcgaatagtt
      901 cggcgcgagg ttgaaggaga tcgtcaccgc gggctgctgc ccctgatgat tgatctgcag
      961 cgggccgacg gtcggtacaa gtttcgcaac cgcgtcgagc gggatggtct ggttgttcgc
     1021 ggtcttcatg tagagcttgg acagatcgga cggatcgacg cggaattcag gctgggcctc
     1081 caggatgatc tgataatcat tggacggcat gtagatggtg ccgacctggc gcccgccata
     1141 ggcgttatag agctggttgc ggacctggtc gacggtgacg ccgtagaccg cggccttctc
     1201 gcgatcgata tcgaccgtca tctgcgggtt cttgatatag aggtcggtag tgacgtcgag
     1261 cagtccgggt atcttcgcaa tcttgtcgcg catttccggc gcaatacgat aaagcgattc
     1321 ggtgtcgccg ctctgcagga cgtattgata ctggctcttg gaaattcgcc cgccgacgct
     1381 caagttctga atgctctgga agaaggcctg catgccggga atctgggtcg ccttctgccg
     1441 caagcgccca atcacggcgg cggcaccgtc tcgttctttc tgcggtttca gcgcgatgaa
     1501 cagccggccg taattcgcgg taggattcgg accacccgaa ccgaccgtgc tgttgatgta
     1561 gtcgatcgcc gggtcggatt tcagcacgtc gaccagcgcc tgctgccgca ccttcatggc
     1621 ctcgaaggag gtatcggtcg cggcttccgt cacgccgatc aggaaaccgg tatcttcctg
     1681 cggaaagaag cccttcggca cgatcatgta gagataaacg gtgccgccga gcgtcgccag
     1741 cgtcaccagc agcatgacag cctttttcgc cagcacccag tcgagcgccc attcatagcc
     1801 gcgaagccag gaatcgaaca cccgttcaaa ggcgcgaagc acgatgttcg gcttcctcgt
     1861 ttcatcatgc gccttcagca cccgcgcgca cagcatcggc gtcagcgtca gcgacacgaa
     1921 tccggaaacg atgatcgcga ccgaaaccgt cactgcgaac tcacggaaca cgcggccgac
     1981 gatgccgccc atcagcagca ccggaatgaa cacggcgatc agcgagaacg tgatggaaat
     2041 gatggtgaag ccgatttccc gcgcgccctt cagcgccgcc tcgtaaggcc gcatgccgtg
     2101 ttcg
//
LOCUS       DATNOI010000192         6173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1301710,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000192 DATNOI010000000
VERSION     DATNOI010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301710"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(426..1478)
                     /locus_tag="VN968_07390"
     CDS             complement(426..1478)
                     /locus_tag="VN968_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL29099.1"
                     /translation="MAASTAAASKSGLYADPREDWLTLRSEEIIDPLRPIVDPHHHLW
                     DRGGQRYLIEEMAQDIASGHNILATVYVEARSMYRASGPEALRPVGEVEFANGAAAMS
                     ASGGYGPAAICAGIVGHANLLLGEGARPVLEAEIAAGNGRFRGIRHSSAWDADPNVAH
                     MYATRPKGLLLDPTFRKGFACLARLGLSFDAWLFHPQIGELTDLARAFPDTKIVLDHC
                     GGPIGIGSYANRRDEIFPAWKASIREIARCPNVVVKLGGLAMRLLGYDFHERPMPPSS
                     EQAAVAWRPYIETCIEAFGPERSMFESNFPPDKGQCSHQVIFNAFKRIAARYSEAEKT
                     ALFSKTATEFYRLKLG"
     gene            complement(1525..1923)
                     /locus_tag="VN968_07395"
     CDS             complement(1525..1923)
                     /locus_tag="VN968_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017170488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXL29100.1"
                     /translation="MRITADTNVLIRAVTGDNARQSKAAQAALKNADVVALAIPALCE
                     LVWVLSQGYKIPQREVAEAIRRLMNGANVVVNRPAAEAGLSLLDAGGDFADGVIAYEG
                     SWLGADAFVSFDKKAVKLIETHGGSARLLP"
     gene            complement(1920..2171)
                     /locus_tag="VN968_07400"
     CDS             complement(1920..2171)
                     /locus_tag="VN968_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019334760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXL29101.1"
                     /translation="MSTLTVTAKGQVTLRKDILRHLGVQPGQKIALDKLPNGRIEVRA
                     ARPAGKISDAFNFLKRKNGPSLSIEEMNKIAARGWAGKR"
     gene            complement(2248..3066)
                     /locus_tag="VN968_07405"
     CDS             complement(2248..3066)
                     /locus_tag="VN968_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL29102.1"
                     /translation="MLRSFSAAFALLVQLTMPAAAQDASRSSECLAMTNAPPRTTPAS
                     LRSAAAKTDEVAITYAGHSTYYIDTPAGVRIATDYSGAYTTGRPPDVVTMNRAHSSHY
                     SLFPDSRIPYVLHGWGEDGQPARIALRVGDVLIRNVTTDIRRYYGDDSSGEMIKDGNS
                     IFIFEVAGLCIGHLGHLHHKLDESHYAAIGRLDIVMVPIDGTYTMSLDGISDITRRLR
                     SSVVLPMHRFMTPLDDFMRRIGQQFEIDTRTARTLKISRDSLPSAPTVIILDGV"
     gene            complement(3125..4685)
                     /locus_tag="VN968_07410"
                     /pseudo
     CDS             complement(3125..4685)
                     /locus_tag="VN968_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605144.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
     gene            complement(4682..5434)
                     /locus_tag="VN968_07415"
     CDS             complement(4682..5434)
                     /locus_tag="VN968_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL29103.1"
                     /translation="MKPVESALHVRGLTKRFDRLAVDALDLTVQPGEFYALVGPNGAG
                     KTTTLRMVAGLSRPDVGSVAVFGVDALSDPVAAKQMMAWVSDEPMIYDKLTPLEYLEF
                     VAGLWGVDPAISGRSANDLLVSLGLEPHLHERCEGFSKGMRQKVALAGALVHDPRLII
                     LDEPLTGLDAVSARHVKGLLTDRVRAGCTVIMTTHILEVAERMADRIGVIASGRLVAE
                     GTLAELRRQNGHNDTSLEDLFIALLNVEAVAA"
     gene            5736..>6173
                     /locus_tag="VN968_07420"
     CDS             5736..>6173
                     /locus_tag="VN968_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL29104.1"
                     /translation="MLDFVQQLVSGIALGCVYGLIALGFVLVYKATEVVNFAQGDLMM
                     LGGFFAFTFIGILGLNYWIGFAAAVAAMALFGMLAERVVVRPILGYPQFSIIMATIGL
                     GYFLRSVVGMIWGTDDFTIETPFSHGVLRIGSLVLADDKLSVIA"
BASE COUNT         1250 a         1915 c         1905 g         1103 t
ORIGIN      
        1 ctccggaaag gaggtgagcg gcccgcgagc acggatcagg taaatacgat tgagagcagg
       61 cccgagcaaa gcaggactag acccgcaacg gtcacaccga ggaaactgta agaagcgaat
      121 tcttcacgca tggtacacca cctatctctc gggaatgctg gttaccgtct ctgctatcgt
      181 gtgccccgct cgaacagaac gactgtcact ttgcacagtc aaaaggagtt tgcgcggtac
      241 cgaaaatcgg tttctgactg ttgttttaga gcaacagggg ccggcccacc ccgaaaatca
      301 gcccacgcgt gtgtgcatgc cgcagtttag agttggccgt tgcgccagcg cggtactgca
      361 ccaaccagga acaaaacggc gtccgctcgg gtcgcaaatg cgaagtgcga cgctaagggg
      421 cgacttcaac ccaatttcag ccgataaaac tccgttgccg tcttcgagaa caatgcggtc
      481 ttctcggcct cgctatatcg cgcggcgatg cgtttgaagg cgttgaagat cacctgatgg
      541 ctgcactggc ccttgtctgg tggaaagttg ctctcgaaca tgctgcgctc gggaccaaag
      601 gcctcgatgc aggtctcgat atagggacgc cacgctacgg ccgcctgttc agacgacggc
      661 ggcatcggac gctcgtgaaa atcataaccc aataaccgca tcgccaggcc gccaagtttt
      721 acgacgacgt tgggacatct ggcgatttcc cggatcgacg ctttccacgc tggaaagatc
      781 tcatcgcgcc ggttggcata gctgccgatg ccgatcggtc cgccgcaatg atcgagcacg
      841 atttttgtat ccggaaaagc gcgagcgaga tcggtgagct cgccgatctg tggatgaaac
      901 agccacgcat cgaaactcaa gccgagccgc gcaagacagg caaagccctt gcgaaaggtc
      961 ggatcgagca ggaggccttt cgggcgagtg gcgtacatgt gggcgacgtt cgggtccgca
     1021 tcccacgcgg aagagtgccg gatgccgcga aagcggccgt tgccggccgc gatttccgcc
     1081 tccagcaccg gtctcgcgcc ttcgccgagc agcagattgg catggccgac gataccggcg
     1141 cagatcgcgg cgggaccata accgccgctc gcgctcatcg cggcggcacc gttggcgaat
     1201 tcgacctcgc ccacggggcg caacgcttct ggcccgctgg cgcgatacat cgagcgagcc
     1261 tcaacataga cggtcgcaag gatgttgtgg ccggacgcga tgtcttgcgc catctcctcg
     1321 atcagatagc gctggccgcc gcgatcccag agatgatgat gcggatcgac gatcggacgc
     1381 agtggatcga tgatttcttc gctccgcaag gtcagccagt cttcgcgcgg atcggcatag
     1441 agcccgctct tcgatgcggc ggcagtgctg gcggccatgg atcgtcactc tctgaatggt
     1501 tgcgatacca aaggtatgcg atatctaggg cagcagtcgc gcggagccgc cgtgtgtctc
     1561 aatcagcttc acggccttct tgtcgaaaga gacaaaggca tccgcgccga gccaacttcc
     1621 ttcataggcg ataacaccgt cggcaaaatc accacctgca tctagcagac tcaatccagc
     1681 ttccgctgca ggccggttca ccacgacatt tgcgccgttc atcaggcgcc ggatcgcttc
     1741 cgcgacctcg cgctgcggaa tcttgtagcc ctgcgacagc acccagacca gttcgcaaag
     1801 cgccggtatc gctagcgcaa ccacgtctgc gttcttaagc gccgcctgag cggctttgct
     1861 ttgccgtgcg ttgtccccgg taaccgcccg aatcagcaca ttggtatcgg cggtgatcct
     1921 catcgtttgc cggcccagcc ccgggcggcg atcttgttca tttcctcaat cgacagcgac
     1981 gggccatttt tccttttcaa gaaattaaag gcatcggaaa tcttgcccgc aggccgagcc
     2041 gccctcacct cgatccggcc attaggaagc ttgtctaggg cgattttctg tccgggctga
     2101 acgcccagat gcctcaggat atccttgcgc agggtgactt gtcccttggc ggtaacggta
     2161 agcgtgctca tggacggcct catttctgag aatgtaaggc aaaaatacct tacaatcaag
     2221 taacctacgg gccagcttcc atagctttca aacaccgtcg agaatgatga ccgtcggcgc
     2281 gctcggcagc gaatcccgcg aaatcttcag ggttcgcgcg gtccgcgtat cgatctcgaa
     2341 ctgctggccg atccggcgca tgaagtcgtc gagcggggtc atgaagcgat gcatcggcag
     2401 cacgacggag gagcgcagcc gccgcgtgat gtccgaaatg ccgtcgagcg acatggtgta
     2461 agtaccgtcg atcggaacca ttacgatatc gagccggccg atcgccgcgt aatggctctc
     2521 gtcgagcttg tgatgcaggt gcccgagatg gccgatgcag aggcctgcga cctcgaagat
     2581 aaagatcgag ttgccgtcct tgatcatctc gccgctcgaa tcgtcgccgt aatagcgcct
     2641 gatgtcggtc gtcacattgc ggatcaacac atcgccgacg cggagcgcga ttcgtgccgg
     2701 ctgtccgtcc tcgccccagc cgtgcagcac atacggaatg cgggaatccg gaaatagcga
     2761 ataatgcgaa ctgtgcgcgc gattcatggt gacgacatcc ggcgggcgac ccgtcgtgta
     2821 ggctccacta tagtcggtcg cgatccgcac tcctgcgggc gtgtcgatgt aataggtcga
     2881 atggccggca taggtgatcg cgacctcgtc ggtctttgcc gcagctgagc gaaggcttgc
     2941 cggcgttgtg cgcggtggcg cgttcgtcat cgcaagacat tcgctgctgc gcgaggcatc
     3001 ctgggcagcc gccggcatgg tcagctgaac cagcaaggca aacgcggcgg aaaaagatcg
     3061 caacatggtg acggtccgtg gtccggcagc ggccacactg tatcgcgcaa tcccgccacg
     3121 gcaatcactt gtccgagagg ctttgcatct ctcggtccgc cggctgggcc cgccgcggac
     3181 taatcttcca ggtggccgcg aggatcccgg cggtcatcaa ccccgtgaca ggcgcgacaa
     3241 gcgggacagc cagcgccagc accgcggttg ccgcccatcc gatcgaggaa aacgcttccg
     3301 cgaaggttgc cagccgcgac gaggtctggc ggcggcgaaa ctggctgcgc cgtgcctgca
     3361 cccgaaacca gagctggatc gcggtcgccg atgcggaggc gacgatgacg ccgaccgcgg
     3421 tgaccgccgc ctgcagcggc gaggcaaaca gcagcgctgt gacgcgcgaa aatcacgcta
     3481 atcgcgatca gcaccacttc gattttagcc cggatgaccc gcgaggccgg gagcggcgcg
     3541 gtcgtcacca aatcggcagc gtcttcgccc gagatcgtca gccatgcaag tccacccgcg
     3601 agctggcctg cggccatcac gatgaccggc gtgatcagca cgatggccaa cgaactgtcc
     3661 gcaaagcttc gccacagcag cagcgccggc ggcaccagat aaagcagctg catcagggtc
     3721 tgcgacacca gccacggatc gcgctgcagc agcaaaaatt ccttccgccg cagcgcctgt
     3781 ggccgtgagc tcgcacgaaa cattgcggcg cgcgagccct ggcgggacgg tgcggcattc
     3841 gccgaagcgc ggacgacgat gtcggcaaac ccgggcgaga atatcgccgt cacgctacca
     3901 agcaggacga ggccgcccgc caacaacggc agcagcgctt cgccgtcgcc aagcgcggcg
     3961 cgcgccggcc accagagcat gctctcaatg ccaggtgcga acgctgccgc ggcatcggaa
     4021 gtcagaaccg taaaccgcga catcgtactg taggacagga tcgcggcgat ctgcagcgca
     4081 atgacgaagc cggcgccgat gatcgcggct agaatttgcg ccaccagccg cgtccggctc
     4141 gggccgatca atcgaaacaa tacgatcgtg actgcaaccg ccaccgccgc cgctgacaat
     4201 ccaaccgcca tcacgacgcc gtaggcggaa aaccatctga ccccaccgcc gatcacgaga
     4261 acgttgacga agggcgttga cagcaacagc gccatcccgg tcacagacag cgcgatagcg
     4321 gcgatgcgga ccgagaacac gttggttagg gcgaccggtg acgacatgat cagatcgaga
     4381 tcggcgcggg cgtaaaacac ccgcgtcacc gattcgatcg cctgcgacag catcaaggcc
     4441 caggccagga atatcgtcgc ggtgatgacg atgagggtgg atttgtcgag cggggcctgc
     4501 agatgcgcaa agcggccgat caccgcatag gccggcaggt gcatgatcgc ggcaaagacg
     4561 atcaggccga tgatggcgcg gcgccttcgc ccgcgacgtc cgcctgtcat catcgcgagc
     4621 cattcacgcc atgccaggcg gaattcgtgc cgggcaaacc acgtcaaggc agtagttgag
     4681 ctcatgcggc gaccgcctcg acattcagaa gcgcgatgaa caggtcctcg aggctggtat
     4741 cgttatgccc gttctgccgg cgcaactcgg caagggtgcc ttcggcgaca agccgtcccg
     4801 aggcgatgac gccgatccgg tcggccatgc gttcggcgac ttcgagaata tgcgtggtca
     4861 tgatcacggt gcagccggcg cgcacccgat cggtgagcaa tcccttgacg tggcgcgcgg
     4921 agacggcgtc aagaccggtg agtggttcgt ccaggatgat cagccgcgga tcgtgtacca
     4981 gcgctcccgc aagcgcgacc ttctggcgca tgcccttgga aaatccctcg cagcgctcgt
     5041 gcagatgcgg ctccagaccg agcgagacca ggagatcgtt tgcggagcgt ccggagatcg
     5101 ccggatcgac gccccaaagc ccggcgacga attcgagata ttccagcggc gtcagcttgt
     5161 cgtagatcat gggttcgtcg gagacccagg ccatcatttg cttggcggcg accggatcgc
     5221 tgagcgcgtc gacgccgaac accgcgaccg aaccgacatc gggacgcgag aggccggcca
     5281 ccatgcgcag cgtcgtggtc ttgccggcgc cgttgggccc gaccagcgcg tagaactcgc
     5341 ctggctggac cgtgagatcg agcgcgtcga ccgcaaggcg gtcaaaacgc tttgttaacc
     5401 ctcgcacatg cagcgcggac tcaactggtt tcatgatgcc gggacaatcc gtggtttcac
     5461 atcggatgcg aaccatggac ccaagatatt tcggcgcggt gaatcctgcg ccgcaaatcc
     5521 cgccaatgct tgcgacttgc ccaaagctgc ggcatttctc cgcaatccag gcttgggaca
     5581 gcaaccagcg ctttgttgac aggggtcggc gctttgggct caataagcgc cggacaatgc
     5641 gaagcttttc agcgcaggcg gcgctggcta gcaagtggac gcaagatgcg ccaaaggcga
     5701 tcgatagaat cgccgcgcgt cgggggagga tggcaatgct ggacttcgtt cagcagctgg
     5761 tgagtggcat tgcgctcggc tgcgtctacg gcctgatcgc ccttggcttc gtccttgttt
     5821 acaaggcgac cgaggtcgtc aatttcgcac agggcgacct gatgatgctg ggcggatttt
     5881 tcgccttcac cttcatcggc attctcgggc tcaattactg gatcgggttt gccgccgcgg
     5941 tagcggcgat ggcgctgttc ggcatgctgg ccgagcgcgt ggtggtgcgc ccgatcctcg
     6001 gctatccgca attttcgatc atcatggcga ccatcgggct tgggtatttc ctgcgctcgg
     6061 tcgtcggcat gatctggggc accgacgact tcacgatcga gacgccattc agccatggcg
     6121 tgttgcggat cggcagcctg gtgctggccg atgacaaact ctccgtgatc gcg
//
LOCUS       DATNOI010000193         7278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2010030,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000193 DATNOI010000000
VERSION     DATNOI010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010030"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1031
                     /locus_tag="VN968_07425"
     CDS             3..1031
                     /locus_tag="VN968_07425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HXL29105.1"
                     /translation="MADALAILMDQPEILPRWAKLQWWLRYLGRHAKQFNPRGRSKNN
                     VAHHYDLDGRLYSLFLDADKQYSCAYFETPDVDLDDAQLAKKRHLAAKLLIGHGNRVL
                     DIGSGWGGLGLYLAEMTGANITGITLSTEQLAVSNARAAEKNLAASAKFFLEDYRDIS
                     GPFDRIVSVGMFEHVGVDFYETYFRQCAELLTDDGVMLLHSIGRSNGPDVTSPWTTKY
                     IFPGGYIPAISEVIPAIERSGLLVCDIEILRLHYAETLKAWRERFMARREEAVRLYDE
                     RFARMWEFYLAGSEMSFRKQNLMNFQIQLSKRQGVVPMTRDYIAHEEAKLRGIERGKQ
                     PRLQIAGE"
     gene            complement(1062..1211)
                     /locus_tag="VN968_07430"
     CDS             complement(1062..1211)
                     /locus_tag="VN968_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29106.1"
                     /translation="MLIDAMEKVSRPEFRAVPCDLSVLAAVRAQLARPAAPYVHGGSR
                     FSLRN"
     gene            1409..1531
                     /locus_tag="VN968_07435"
     CDS             1409..1531
                     /locus_tag="VN968_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29107.1"
                     /translation="MAQKTYPSSFDPQRDIGTDYALIAIGVGAALIALLYLLLI"
     gene            1829..3328
                     /locus_tag="VN968_07440"
     CDS             1829..3328
                     /locus_tag="VN968_07440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HXL29108.1"
                     /translation="MAPFDSNVLKLAPDSGTPAYRSAPHNIEAEQSLLGAILVNNDAF
                     YRVSDFLEPKHFFEPIHQTIYETAGSLIRMGKVATPVTLKTFVPADTDIGGMTVGQYL
                     ARLAAEATTIINAQDYGRTVYELALRRDLIRIGEDMVNVAFDAPVDFAPRAQIEDAER
                     RLYELAESGRYDGGFQRFAQALTVAVDMAAKAFQRDGKLSGIATGLRDLDTKMGGLQS
                     SDLIIVAGRPGMGKTALATNIAYNVAKAHRAEVQADGTMKSVNGGIVGFFSCEMSAEQ
                     LATRILAEQTSISSSSIRRGGITEADFEKIRDYSIELQSLPLYVDETGGLSISQLTAR
                     ARRLKRQKGLDLIVVDYIQLLQGSGKRSDNRVQEVTEITTSLKALAKELNVPVIALSQ
                     LSRQVESRDDKRPQLSDLRESGSIEQDADVVMFVFREEYYLANKEPRVGTPEYEKWQL
                     DMSLVHGKAEVIIGKQRHGPTGTVELQFEGQFTRFGDLAQDSHLPVQTY"
     gene            3377..4642
                     /gene="alr"
                     /locus_tag="VN968_07445"
     CDS             3377..4642
                     /gene="alr"
                     /locus_tag="VN968_07445"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXL29109.1"
                     /translation="MNIAPDPKSIPTSTVLSPEANQAAALATAPGVLTVDLDAIVANW
                     RKLEKTAVPAECAGVIKADAYGCGVGPVARALAGAGCKTFFVATLGEARAARAALPSA
                     AIYVLSGFFQNSGDAFAGIDARPVIGDLNELAEWDVFCRRSGWAGGAAIHIDTGMNRL
                     GLTVAEAQGIIPRITAGDHGITLVMSHLACAEILHHPLNAKQLATFREIASLFSGVPA
                     SLSNSSGVFLGGQFQFDMVRPGAALYGVNPTPEADNPMQPVVELKARIAQIRNVEKGE
                     TVGYGGTWTARRPTKLAIVSVGYADGYFRAGSSNDGTRGADVVVAGKRCPVAGRISMD
                     LMAIDITDLAHNAARRGHLVTLIGEGITVDELAHHFGTIGYEVLTSLGPRYARVYKGG
                     TAATPEPPAPVAEPVSEAVAVGSTSAAPA"
     gene            4658..5428
                     /locus_tag="VN968_07450"
     CDS             4658..5428
                     /locus_tag="VN968_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXL29110.1"
                     /translation="MKIATFNINNINRRLPNLLRWLRAAKPDIVSLQELKSTDADFPL
                     SAIEKAGYGAVWRGQKTWNGVAILARNADPVLIRTELPGDADDHEARYIEAAVSGIIV
                     TSIYLPNGNPQPGPKFDYKLDWFRRLKLHAAKFLKRDVPVVLAGDYNVAPTELDIYPT
                     RSWDKDALIQPKSRAAFKSLVAQGWCDAIRERHPSKPMFTFWDYKRNRWPRDAGLRLD
                     HLLLSPAVAPRLIKAGVDRKVRGEEGASDHAPAWIVLS"
     gene            complement(5425..5700)
                     /locus_tag="VN968_07455"
     CDS             complement(5425..5700)
                     /locus_tag="VN968_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29111.1"
                     /translation="MSNALAECDAGFPWLAGAMLAFESCEVIHLRLAKLAIGGHDAGC
                     EAQLMVNEKINAMFEAGASLMSGASAAAVIGRYREHVAANAKRLSVV"
     gene            complement(6698..7066)
                     /locus_tag="VN968_07460"
     CDS             complement(6698..7066)
                     /locus_tag="VN968_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXL29112.1"
                     /translation="MTLRGSCHCGDTQFEVSEAPASVTRCTCSLCAKRGALWAYYTPA
                     QFRLTTPMEKVATYRWGSRTVKHHFCANCGCGTYSESPDWSTGKPDFENPRVGVNSRL
                     LNDFDLDAVPVTVIDGKNLW"
BASE COUNT         1431 a         2339 c         2149 g         1359 t
ORIGIN      
        1 cgattgcgga tgcgctggcg atcctgatgg atcagcctga aatcctgcca cgctgggcca
       61 agctgcaatg gtggcttcgt tatctcggca ggcatgccaa gcagttcaat ccccgcggcc
      121 gctcgaaaaa caatgtcgca caccattacg acctcgacgg gcggctctat tccctctttc
      181 tcgatgccga caagcaatat agctgcgctt atttcgagac accggatgtg gaccttgacg
      241 atgcccaact cgccaagaag cgccatctcg ccgcaaaact gttgatcggg catggcaacc
      301 gcgtgctcga cattggttcc ggctggggcg gacttgggct gtatctggcc gaaatgaccg
      361 gcgccaatat caccggcatc acgctgtcga ccgaacaatt ggcggtgtcg aacgcccgcg
      421 cggccgagaa aaatctggcg gcgtcggcga agtttttcct ggaggactat cgcgacattt
      481 ccggcccatt cgaccgcatc gtgtcagtgg gcatgttcga acatgtcggc gtcgacttct
      541 acgagaccta tttcagacag tgtgccgaac ttcttaccga cgacggcgtt atgttgctgc
      601 attcgatcgg ccgttcgaac ggacccgacg ttaccagccc ctggaccacg aaatacattt
      661 ttcccggcgg ttacatccct gcaatctccg aagtgatccc tgcgatcgag cgttccggac
      721 tgctggtgtg cgacatcgaa atcctgcggc tgcattacgc cgaaacgctg aaagcgtggc
      781 gcgagcgttt catggcgcgg cgagaagaag cggtgcggct ctatgacgag cgctttgcgc
      841 ggatgtggga attttacctg gcggggtcgg aaatgtcgtt ccgcaagcag aacctgatga
      901 atttccagat ccagctctcc aaacgccagg gcgtggtgcc gatgacgcgg gattacatcg
      961 cacatgagga agcgaaactg cgcgggatcg agcgcggcaa gcaaccgcgg ctgcaaattg
     1021 caggcgaata ggcccgtagc gcaaaatctg gtttccagaa tttagtttcg gaggctgaac
     1081 cgggatccgc catgaacgta cggcgccgcc ggacgcgcca gttgcgcccg caccgccgcc
     1141 agcaccgaca ggtcgcaagg caccgcgcgg aattccggac gggagacctt ttccatggcg
     1201 tcgatcagca tcgacaacca tttagtctgg ccgttttccg atcgccatgg ctgaagttgc
     1261 tggcccatca cctgttccct gccgcaaaag gcgacattac aaaccctgat gtcaccaacc
     1321 cgttcccgaa gaccgtacaa ttgtagaaaa tccgccaaat gtgatctgct tcacagatgg
     1381 accggccggc ttggcctaaa tgtcccaaat ggcccagaag acgtacccat catcgttcga
     1441 tccgcagcgc gacatcggca ccgactatgc cttgatcgcc attggcgtcg gggctgccct
     1501 gatcgccctg ctctatctcc tcctgatttg attcagcggt gagatggcgg ttcggaatat
     1561 tcctcgcccg attgggatga gtgtccccgg cgcggcgaaa agttcaggct gcgccgaaat
     1621 tttttctttt attcaggcga tgggacgagc ctcgcccgag gtctgcccga atcccgtcaa
     1681 gcgccaacac gcggcccggt cgggattaac acattgcagt gtgtgaatcg ggcataaggc
     1741 gcgttgccgc ttccgtgatg tccgggagtg gtgcttgatc gccgcccccg ccccgtatcc
     1801 gacaaagtcc caacaacact cgagaaccat ggccccattt gattcgaacg ttctcaagct
     1861 cgcacccgac tcgggaactc cggcctatcg gagtgcaccg cacaatatcg aagcggagca
     1921 gagcctgctg ggtgcgatcc tggtcaacaa cgacgcgttc tatcgagtgt cggattttct
     1981 ggaaccgaaa catttcttcg agccgatcca tcagaccatc tacgagaccg ccggcagcct
     2041 gattcggatg ggcaaggtcg ccacccccgt gacgctgaag accttcgttc cggccgacac
     2101 cgacatcggc ggcatgacgg tcggccaata tctcgcccgc ctcgccgcgg aagcgaccac
     2161 catcatcaat gcgcaggatt atgggcgcac ggtctacgaa ctggcgctgc gccgcgacct
     2221 gattcggatc ggcgaggaca tggtcaatgt cgccttcgat gcgccggtgg atttcgcgcc
     2281 gcgcgcgcag atcgaggatg ccgagcgcag gctgtacgaa ctggccgaat ccggccgcta
     2341 cgacggcggc ttccagcgct tcgcgcaggc cctcaccgtc gccgtcgaca tggcggcaaa
     2401 ggcgttccag cgcgacggca aattgtccgg cattgcaacg ggtttgcgcg atctcgatac
     2461 caagatgggc ggcctgcaat cctccgatct gatcatcgtc gccggccgtc ccggcatggg
     2521 caagaccgcg cttgccacca acatcgccta caatgtagcc aaggcgcatc gcgccgaagt
     2581 ccaggccgac ggcaccatga agtccgtcaa cggcggcatc gtcggcttct tctcctgcga
     2641 aatgtcggcc gagcagctcg ccacgcgtat tctcgccgaa cagaccagta tctcgtcgag
     2701 ttcgatccgg cgcggcggca ttaccgaagc cgatttcgag aagatccgcg actactccat
     2761 cgaattgcag tcgctgccgc tttatgtcga cgaaaccggc ggcctttcga tttcgcagct
     2821 gaccgcgcgc gcgcggcggt tgaagcgcca gaaaggcctc gatctgatcg tggtcgacta
     2881 tatccagctg ctgcagggat cgggcaagcg ttccgacaac cgagtgcagg aggtgaccga
     2941 gatcaccacc agcctgaagg cgctggccaa ggaattgaac gttccggtca tcgcgctgtc
     3001 gcagctgtcg cgccaggtcg aaagccgcga cgacaagcgc ccgcaactgt ccgacctgcg
     3061 tgaatccggc tcgatcgaac aggacgccga cgtggtgatg ttcgtgtttc gcgaggaata
     3121 ttacctcgcc aacaaggagc cgcgggtcgg cacgcccgag tatgaaaaat ggcagctcga
     3181 catgtcgctg gtgcacggca aggccgaagt catcatcggc aagcagcgcc atggcccgac
     3241 cggtacggtc gaactgcagt ttgagggcca gtttacgcgg ttcggcgatc tcgcgcagga
     3301 cagccatttg ccggtgcaga cctactgatc cggcctgccc ggttgaaccg ccgggccccc
     3361 gcgcgtagtg tgcggcatga acatcgctcc cgacccgaaa tcgatcccca ccagcaccgt
     3421 gttgtcgccg gaagcaaacc aggcggccgc gcttgcgacc gcgcccggcg tgctcacggt
     3481 cgatcttgac gccatcgttg cgaactggcg caagctggag aaaaccgcgg tgccggccga
     3541 atgcgccggc gtgatcaagg cggacgccta tggttgcggg gtaggtccgg tcgcccgcgc
     3601 gctcgccggc gccggctgca agacgttttt cgtcgccacg ctgggcgaag cccgcgccgc
     3661 ccgcgcggcg cttccctcgg cggcgatcta tgtgctcagc ggcttcttcc agaattccgg
     3721 cgacgcgttt gccgggatcg acgccaggcc ggtgatcggc gatctcaatg aactcgccga
     3781 atgggacgtg ttctgccgcc gctccggctg ggccggcggt gcggccattc atatcgacac
     3841 cggcatgaac cggctcggcc tcaccgtcgc ggaggcgcag ggcatcattc ccaggatcac
     3901 tgccggcgat cacggcatca cgctggtcat gagccatctt gcctgcgccg aaatcctcca
     3961 tcatccgctc aacgccaagc agctcgcaac cttccgcgag atcgccagtc ttttctccgg
     4021 ggtgccggca tcgctgtcga attcctccgg ggtttttctc gggggccaat tccagttcga
     4081 catggtgcgg ccgggggcag ccctttacgg cgtcaatccg acgcccgagg ccgacaatcc
     4141 gatgcagccg gtcgtcgaac tgaaggcccg catcgcgcag atccgcaacg tcgagaaagg
     4201 cgaaaccgtc ggttacggcg gcacctggac cgcgcggcgg ccgaccaaac tcgcgatcgt
     4261 atcggtcggc tatgccgacg gatatttccg cgccggcagc agcaatgacg gcacccgcgg
     4321 cgccgacgtc gtggtcgccg gcaagcgttg cccggtggcg ggccgcatct cgatggacct
     4381 gatggcgatc gacatcaccg atctcgccca taacgcggca cggcgcggcc atctggtgac
     4441 gctgattggc gagggcatca cggtcgacga gcttgcgcac catttcggca ccatcggcta
     4501 cgaggttttg accagcctcg gcccgcgcta tgcgcgggtt tacaagggcg gcacggcagc
     4561 gacgccagaa ccgcccgctc cggtggccga gccggtctcc gaggcagtgg ccgtgggatc
     4621 gacatccgca gcacctgcct gacgtcttca gtcaggcatg aagatcgcca ccttcaacat
     4681 caacaacatc aaccggcggc tgcccaacct gttgcgctgg ctgcgcgccg caaaacccga
     4741 catcgtttcc ctgcaggaac tgaaatcgac cgacgccgat tttccgcttt ctgcgatcga
     4801 gaaggccggc tacggcgcgg tctggcgcgg ccaaaagacc tggaacggcg tcgccatttt
     4861 ggcgcgcaac gccgatccgg tgctgatccg gaccgagttg cccggcgacg ccgacgacca
     4921 cgaggcgcgc tatatcgagg ctgccgtcag cggcatcatc gtcaccagca tctaccttcc
     4981 gaacggcaat ccgcagcccg ggccgaaatt cgactacaag ctcgactggt tcaggcggct
     5041 caagctgcac gccgcgaaat tcctcaagcg ggatgtcccg gtcgtgctgg cgggcgacta
     5101 caacgtcgcg ccgacggagc tggatatcta tccgacgcgg tcatgggaca aggatgcgct
     5161 gatccagccg aagagccgcg cggccttcaa atcgctggtc gcgcaaggct ggtgtgacgc
     5221 gatccgcgaa cgccatccgt ccaagccgat gttcacgttc tgggattaca agcgcaaccg
     5281 ctggccgcgc gacgccggct tgcggctcga tcatctcttg ctgagtccgg cggtcgcacc
     5341 gcggctgatc aaggccggcg tcgaccgcaa ggtgagaggc gaagaaggcg ccagcgacca
     5401 cgcgccggcg tggatcgttt tgagctagac cacggaaagc cgttttgcgt tggcggcgac
     5461 atgctcgcgg tagcggccga tgacggccgc agccgacgcg ccggacatca ggctcgcacc
     5521 ggcctcgaac atcgcattga tcttctcgtt caccatcaac tgggcctcgc atcccgcgtc
     5581 atggccgccg atcgccagtt tcgccagccg aagatggatg acttcgcagg actcgaatgc
     5641 cagcatggcg ccggcgagcc agggaaagcc ggcatcacat tccgcaaggg cattgctcat
     5701 cgcgtttcca gacttttgaa ttcgagatga gtttaatacg cgtcaaattc taaggaccaa
     5761 ggcgcgagac cgctgacatt ttcggcgcgg gagccacggc cggttaacca cgagggcgcg
     5821 acgaacttct cgtcattgcg aggagcgaag accgtcatac caattgagat cgtcgtcacc
     5881 gggcgaaagc gcgaagcgcg tcttcgcgct agatgacccg gtaatccatc tttcttcgaa
     5941 aggattttct tacgaagatt gatggatgcc cggatcaagt ccgggcatta cgggtgcgtt
     6001 ttgtggagca agcacttcgc acacacacac acacacacac acacacacac acaccgactt
     6061 cgaacttttc aaacagcaaa accaattcca gacgcggctt cacgatctcg cggcgcgatg
     6121 cgcccgaggt ttgcataaat cttccgcccc ctttgagggc gtggggaatg ccgggtgccc
     6181 gctgcacccg cggcctcgtg tgcactttgt attggtggaa agcacacgag taacaacgag
     6241 tacaccggaa tcgcccggca ttcccgcacg caatggtttt acggcttatg tcgcgctctc
     6301 cccggtgacc gggctctttt gccaccgtcg cctgcggaaa cttggtttgt ccgcgcccgg
     6361 caggggccga cttgccatcc cgcaaaactt gacgccagcg tcggggcgtc aggaccacac
     6421 gattttgccg tccgctgcaa catctctcgt ccgctcgctt ggtaatcact cacaggcctc
     6481 atcgacctgc cctgcaatcc cgtcgcgcgc aaaacgctgc cgcgtccacc gcaccccatc
     6541 ccgcgttcgt gacgatcgcg atacgcccct ctgtgtggga cgggatggcg cgaatttgta
     6601 gagatgattt gcccgacggg tgaagcgaaa tatttttgca gacagggctg gacacacctg
     6661 tccacaagcc acccgacggg caaatcaccg cacggcctca ccacagattc ttgccgtcga
     6721 tcaccgtcac cggcacggcg tccagatcga aatcattcaa tagccgcgaa ttgacaccga
     6781 cgcgcggatt ctcgaaatct ggcttgccgg tcgaccagtc cggcgactcg gaataggtgc
     6841 cgcaaccgca gttggcacag aaatggtgtt tgacggtacg gctcccccat cgataggtcg
     6901 caactttctc catcggtgtg gtcaggcgaa actgcgccgg cgtgtagtag gcccacaacg
     6961 caccgcgctt cgcgcagagc gagcacgtac agcgcgtcac actggccggc gcctcgctca
     7021 cttcaaattg cgtgtcgccg caatggcagc tgcccctgag cgtcatggcc cgatcccctt
     7081 ttcgattggc aaagtgccta tatccttgct gtgggtagct aatcgaaccc tgctgccagc
     7141 gttgtgtcag caatgatttg ccgctgcctc atggtgagga ggcgctcttg cgccgtctcg
     7201 aaccatgagg ccaatgtgca gcactcatcc ttcgagacgc ggcaaagacg ccgctccttt
     7261 agaatgagcg cagttgcg
//
LOCUS       DATNOI010000194         5018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2496949,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000194 DATNOI010000000
VERSION     DATNOI010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2496949"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            267..1946
                     /locus_tag="VN968_07465"
     CDS             267..1946
                     /locus_tag="VN968_07465"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HXL29113.1"
                     /translation="MTSTVPQASTILASLGQAAFVWDIATDAIAWSDHAAAVFPDIPA
                     AALASGAGFAKLIEPVASIRTDALGHSSSARGGTAYRIEYGVRTSTSAPVLWIEESGC
                     WFAGADGRPARAQGIVRVNNERHAHDEQLVRLLRHDPLTGELNRTHLVASLAESIEEA
                     ARFRASSAFMLIGIDRLARINDAFGFDVADAVISEVATRIRARLRNGDVLGRFSGNKF
                     GLILKNCTVEDVNIAAERFLAGIRDDVVPTRSGPVSVTASIGAVSVPRYARNADEAVN
                     RAQETLDMAKRRAGSFSLWRPSVERDAQRRVNIRVTDEIVTALNEGRIAMAFEPVVEA
                     RSRDTAFYECLVRMEQDDGQVLLAPDIVPVAEKLGLIRLVDHRVLELVVAELAGSVNV
                     QLSLNISPDTTMDPDWWASIESLMRAHPGVAERLIIEITETVAIQDIDGLRGFVTRLK
                     NFGSRIAIDDFGAGYTSFRNLRKLGVDIVKIDGAFVQNIARSADDRAFVQTLIDLARR
                     LEIKTVAEWVQDDESAALLRDWGCDYIQGRLIGLASAARPWEKRAEVPAAS"
     gene            complement(2108..2713)
                     /gene="phaR"
                     /locus_tag="VN968_07470"
     CDS             complement(2108..2713)
                     /gene="phaR"
                     /locus_tag="VN968_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HXL29114.1"
                     /translation="MAKTDQPTTIKKYANRRLYNTGTSTYVTLEDLASMVKEGEDFLV
                     YDAKTGDDITRSVLAQIIFEQENKAGQNLLPTTFLRQLIRFYGDSMQMVVPRYLEQSI
                     DTLTREQEKFRKQLTTTFSMTPFAPLEEHVRRNMEMFERTFSMFKPFAAPRAENAGGT
                     EPEKAASEPAAQEDNIDDLRRQMKEMQDRLERMSKEPKKEE"
     gene            3019..4197
                     /locus_tag="VN968_07475"
     CDS             3019..4197
                     /locus_tag="VN968_07475"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXL29115.1"
                     /translation="MSDDVVIVSAARTPVGSFNGAFATMPAHDLGAIAIKAALERGGI
                     EPARVSEVIMGQILTAAQGQNPARQASIAAGIPVESPAWGVNQLCGSGLRTVALGYQA
                     LLNGDSEIVVAGGQESMSMAPHAQYLRGGVKMGPVEFIDTMIKDGLWDAFNGYHMGNT
                     AENVARQYQITRAQQDEFAVGSQNKAEAAQKAGRFRDEIVPVTIKTRKGDIVVSDDEY
                     PRHGATIEAMGKLKPAFEKEGTVTAGSASGINDGAAAVVLMTAKQAAKEGKKVLGRIV
                     SWGQAGVDPKIMGTGPIPASRAALKKAGWSIGDLDLIEANEAFAAQACAVNKDLGWDT
                     AKVNVNGGAIAIGHPVGASGARVLVTLLHEMQKRDAKKGLATLCIGGGMGIAMCIARD
                     "
     gene            4372..>5018
                     /gene="phbB"
                     /locus_tag="VN968_07480"
     CDS             4372..>5018
                     /gene="phbB"
                     /locus_tag="VN968_07480"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HXL29116.1"
                     /translation="MARVALVTGGTRGIGAAISKALKAADYKVAASYAGNDAAADKFK
                     AETDIPVYKWDVSSFEACAAGVKQVEADLGPVDVLINNAGITRDGAFHKMTLEQWNAV
                     INTNLGSLFNMTRQVIEGMRARKFGRIINISSINGQKGQFGQVNYSAAKAGDIGFTKA
                     LALENAKGGVTVNAICPGYINTEMVQAVPKEVLEKSILPLIPVGRLGEPDEIARAV"
BASE COUNT          951 a         1533 c         1597 g          937 t
ORIGIN      
        1 acggcgccat catccagggt ctgctgtggt cggccactgt ttgacatcgc ttgatcaaaa
       61 cccggctgaa ctgtcacgtt ttgggacgtt gcgccccgtt tgccacatgg cgatcgcaag
      121 agccatgccg gtcctccccg ctgtgctccc ggcctgcaaa aacgtggttt ggcggaatat
      181 tcatgaactt tgcctagggt gagggccgac gcaggcagta tcctcggacc gggaatcacc
      241 cgggggtgtc ccgaaacgag gcgattttga catccaccgt tccgcaagct tcgaccatcc
      301 tggcctcgct cggccaggcg gccttcgtgt gggacatcgc aaccgatgcg attgcctgga
      361 gcgatcacgc cgccgcggtt tttccggata ttccggcggc ggcgctggca agcggcgctg
      421 ggtttgcaaa actgattgag ccggtggcct cgatccggac cgacgcgctt ggccattcat
      481 cgtccgcgcg cggtggcacg gcctatcgaa tagagtatgg cgtacggacc agcacatcag
      541 ctcccgtgct ctggatcgag gagtccggct gctggttcgc cggtgctgac ggcaggccgg
      601 cgcgcgcgca aggcatcgtc cgcgtcaata atgagcggca tgcccacgac gagcaactgg
      661 tgcggttgtt gcgccacgat ccgctcaccg gcgagctcaa tcgtacgcat cttgttgcat
      721 cgctggccga gtccatcgag gaagccgcgc gcttccgcgc ctcttccgcc ttcatgctga
      781 tcggcatcga tcgtctggcg cgcatcaacg acgccttcgg cttcgacgtc gcggacgcgg
      841 tgatttccga ggtcgcgacc cgcattcgcg cgcggctgcg caacggcgat gtgcttggac
      901 gattctccgg caacaaattc ggactgatct tgaagaactg caccgtcgag gacgtcaata
      961 tcgccgccga gcggtttctg gccggcattc gcgacgacgt ggtgccgacc agatccggtc
     1021 ccgtgtccgt gacggcatcg atcggcgcgg tcagcgtgcc gcgttacgcg cgcaacgccg
     1081 acgaggccgt caaccgcgcc caggaaacgc tcgatatggc gaagcgacgc gccggatcgt
     1141 tttcgctgtg gcgcccgagt gtcgagcgcg acgcccagcg ccgcgtcaat atccgcgtca
     1201 ccgatgaaat cgtcaccgcg ctcaacgagg gccggatcgc gatggcgttc gagccggtgg
     1261 tggaggcccg ctcgcgcgac accgcgttct acgagtgtct cgtccgcatg gagcaggatg
     1321 acgggcaggt tctgctggcg ccggacatcg tccccgtagc cgagaagctc ggcctgatac
     1381 gtctggtcga tcaccgcgtg cttgaactcg tggtggccga actcgcaggc tcggtgaacg
     1441 tgcagctcag cctcaacatc tcgcccgata ccacgatgga cccggactgg tgggcttcaa
     1501 tcgaatcgct gatgcgcgcg catcccggcg tggccgagcg gctgatcatt gaaatcaccg
     1561 agacggtcgc gatccaggat atcgacggcc tgcgcggctt tgtcacgcgg ctgaaaaact
     1621 tcggcagccg gatcgcgatc gacgatttcg gcgccggtta cacctcgttc cggaatttgc
     1681 gcaagctcgg cgtcgatatc gtgaagatcg acggcgcctt cgtgcagaac atcgcccgct
     1741 cggccgacga tcgcgccttc gtgcagacgc tgatcgacct ggcgcgccgg ctggaaatca
     1801 agacggtcgc ggaatgggtt caggatgacg aatccgcagc actgctgcgc gactggggct
     1861 gcgactacat ccaggggcgg ctgatcggcc ttgcgtccgc ggcgcgtccc tgggaaaagc
     1921 gggctgaagt gccggcggcg agctaaaata tccgccgtcg tcccggcgaa cgccgggacc
     1981 tataaccccc ggcattattt gttgcagaag gcctctgcca catcgctcta tcgatagaac
     2041 acaatgtatg ggcccggcgt tcgccgggac gacgtgcgga gagtttcgaa caggcggcgc
     2101 gccgcgctca ctcttccttc ttcggctctt tcgacatccg ttcgagacgg tcctgcattt
     2161 ccttcatctg acggcggaga tcgtcgatgt tgtcttcctg tgcggcgggc tcggaggcgg
     2221 ctttctcagg ctcggtgccg ccggcgtttt cggcgcgggg ggctgcgaat ggcttgaaca
     2281 tcgagaaggt tcgctcgaac atttccatgt tgcggcggac atgctcttcc agcggcgcga
     2341 atggcgtcat gctgaaggtg gtggtgagct gcttgcggaa tttttcctgc tcgcgcgtca
     2401 gcgtgtcgat ggactgctcg agatagcgcg gaaccaccat ctgcatgctg tcgccgtaga
     2461 atctgatcaa ctgccgcagg aaggtggtcg gcagcaaatt ctggccggcc ttgttttcct
     2521 gttcgaagat gatctgcgcc aataccgagc gcgtaatgtc atcgccggtt ttggcgtcat
     2581 agacgaggaa gtcttcgcct tccttcacca tcgacgcgag gtcttcgagc gtcacatagg
     2641 tgctggtgcc ggtattatag agccgccggt ttgcatattt cttgatcgtg gtcggttggt
     2701 ctgttttcgc catgggctca cacatgcaca ccggagagga cgggaacccg acggcatcgc
     2761 cgaagggcaa gcgagcaacg caatcgcaac aaagtaagca ttttcaattg agttcggcta
     2821 ccgttttgtg cgacacggtt aattccaaag cataggcatt gctcaggaaa ctgccaagaa
     2881 cgctattttg aatgcgccgc ggcgtgaatt cgccctctgg ccgattgaca tgccttaacg
     2941 acgttaacag gataaagtga gagacaggcc tgccatccgg ccgcccgccg tcaagatcct
     3001 caagcccagg agatgtccat gtcagacgat gttgtcatcg tcagcgccgc ccgcaccccg
     3061 gtcggcagtt tcaatggcgc gttcgccacc atgcccgccc acgatctcgg cgcgatcgcc
     3121 atcaaggcag cgcttgagcg cggcggcatc gagccggcgc gggtgtccga ggtgatcatg
     3181 ggccagattc tgaccgcggc ccagggccag aacccggccc gtcaggcctc gatcgccgcc
     3241 ggcatcccgg tggaaagccc ggcctggggc gttaatcaat tgtgcggttc gggcctgcgc
     3301 acggtggcgc tgggctatca ggcgctgctc aacggcgatt ccgaaatcgt cgtcgcgggc
     3361 ggccaggaat cgatgagcat ggccccgcac gcccaatacc tgcgcggcgg cgtcaagatg
     3421 ggcccggtcg aattcatcga caccatgatc aaggacggcc tgtgggacgc cttcaatggc
     3481 taccacatgg gtaacaccgc cgagaacgtc gccaggcagt atcagatcac gcgcgcccag
     3541 caggacgaat tcgcagtcgg ctcgcagaac aaggccgagg ccgcgcagaa ggccggccgt
     3601 ttcagggacg agatcgttcc ggtcaccatc aagacccgca agggcgatat tgtcgtcagt
     3661 gacgatgaat acccccgtca cggtgcgacg atcgaagcca tgggcaaact caagccggcc
     3721 ttcgaaaagg aaggcaccgt caccgccggc agcgcttccg gcatcaatga cggcgccgcc
     3781 gcggtggtgc tgatgaccgc gaagcaggcg gccaaggaag gcaagaaagt gctggggcgg
     3841 atcgtgtcgt ggggccaggc cggcgtcgat cccaagatca tgggcaccgg ccccatcccg
     3901 gcctcgcgcg ccgcgctgaa aaaggccggc tggagcatcg gcgacctgga tttgatcgag
     3961 gccaatgagg ccttcgcggc gcaggcctgc gcggtcaaca aggacctcgg ctgggatacc
     4021 gccaaggtca acgtcaacgg cggcgcgatt gcgatcggcc acccggtagg cgcctccggc
     4081 gcccgcgtgc tggtgacgct gctgcatgaa atgcaaaagc gcgacgccaa gaagggcctc
     4141 gccacgctgt gcatcggcgg cggcatgggc atcgcgatgt gcattgcacg cgactaatca
     4201 tgcggcgctg catgatgaaa agatcatctc gcagctgcgt ccgttcttgg taagataaag
     4261 cgcccggcct aagtgccggg cgttttattc ttgatgttgg tcaatccgag cgtttaattc
     4321 ggcgttacat aaaaagtgta gctcaagaag acgtttaggg agggattcaa tatggcacgt
     4381 gtcgcattgg tgacgggggg aacacggggc ataggtgccg caatcagcaa ggcgctgaag
     4441 gccgccgact acaaggttgc cgcgagctat gcgggcaacg acgccgcggc ggataaattc
     4501 aaggccgaga ccgacatccc cgtctacaaa tgggatgtca gttcgttcga ggcctgcgcc
     4561 gccggcgtca agcaggtcga ggccgacctc ggcccggtcg acgtgctcat caacaatgcc
     4621 ggcatcaccc gcgatggcgc attccacaag atgacgctcg agcaatggaa tgcggtgatc
     4681 aacaccaatc tcggttcgct gttcaacatg acgcgtcagg tgatcgaagg catgcgcgcc
     4741 cgaaaattcg gccgcatcat caatatctcc tcgatcaacg gccagaaggg tcagttcggc
     4801 caggtcaatt attccgccgc caaggccggc gatatcggct tcaccaaggc gctcgcgctg
     4861 gagaatgcca agggcggcgt cacggtgaat gcgatctgcc ccggctatat caacaccgag
     4921 atggtgcagg cggtgccgaa ggaggtgctg gagaaaagca tcctgccgct gatcccggtg
     4981 ggtcgcctcg gcgagcccga cgagatcgcc cgcgccgt
//
LOCUS       DATNOI010000195         5649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_363608,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000195 DATNOI010000000
VERSION     DATNOI010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363608"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(399..956)
                     /locus_tag="VN968_07485"
     CDS             complement(399..956)
                     /locus_tag="VN968_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXL29117.1"
                     /translation="MNAHVSQGGWPALVLNADFRPLSYYPLSLWSWQDAIKAVFLDRV
                     NIVEQYDRAVRSPSFEIQLPSVVSLKSFVKPSTHPAFTRFNVFLRDRFVCQYCSSNDD
                     LTFDHIIPRSKGGHTTWENVVAACSPCNLRKGNLTPQQARMFPRQFPFAPTVHQLHRN
                     GRLFPPNYLHDSWLDYLYWDTELDP"
     gene            1150..2910
                     /locus_tag="VN968_07490"
     CDS             1150..2910
                     /locus_tag="VN968_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HXL29118.1"
                     /translation="MLTTSRYLEAPRRLRAFVRAHETSLVVLAALVGTIGGWVVLAMS
                     LAVAALHAVLFNIDIRDRLSSQLGIDPLRAVLVPSLGGLVLGLALLLLQRWRPAREID
                     PIEANALHGGRMSFRGSVIVALQTVWSSGVGASVGLEAGYTQLASGISASLGRAFHLR
                     RADQRIMVGCGAAAAIAGAFGAPLAGAFYAFELVIGGYTPASLTAVGVAAVAGYFVTH
                     GFAALSLGIGVGPVGDVLGRDLALAGLLGILAALFGIAIMRGVALCESLLTGSGIWLP
                     LRPALGGLAVGLMALLTPHVMSSGHGALHFTSIVSMPLTMIAGVFVLKALASVISLGS
                     GFRGGLFFAALFLGALGGRLFAAGIDMIWPGLNLDPNVYAIVGMSALSASVIGGPLTM
                     AFIALESTGNLWLTTAVLVAVIISTQITRELFGYSFATWRFHLRGETIRSAADVGWIR
                     DLTVRSLMRPDVPTVNADMPIGQFRGRFPLGSKTQVVAVDAAGRYAGLALVADAHAPG
                     LDAKSGLNGILHHRDVVLHPVMNIQEAIEVFDAAEAESLVVVESGDEHRPIGILTEAH
                     AMRRYAEESEQRRREAVGDV"
     gene            3217..4194
                     /locus_tag="VN968_07495"
     CDS             3217..4194
                     /locus_tag="VN968_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL29119.1"
                     /translation="MGRLLTVLVATLLGSGSIDMAAAQNYPARPIAMIVPFPAGGATD
                     TLARFLSEKMRGILGQALIIENVAGAAGSIGVGRAVRSPADGYTLSIGTSTTHMLTGG
                     LYALQFDLLRDLEPIILIGSEPLLIVGRKSLPADDLKGLIAYLKANPDKASVGIAGVG
                     ATGHLTGISFQKATGTKFQFVPYRGNAPAMQDLLAEQIDFMIEPSSNFKSLVGAGSVK
                     PYAITGRARLASSPDIPTADEAGLPGFLASLWYGLWVPRDTPKDIIARLNATMVQVLA
                     DPSVKERFAELGIQIAPLAQQSPEALRAFQKAEAERWWPIIKAANLKAE"
     gene            complement(4200..5168)
                     /locus_tag="VN968_07500"
     CDS             complement(4200..5168)
                     /locus_tag="VN968_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HXL29120.1"
                     /translation="MPVILGSGEHRYRVVENWAKLPEGWNLSDVASVAVDSKDRVYVF
                     NRGAHPMVVLDREGNFITSWGEGLFNRAHGLHIDTEDNLYCTDDGDHTVRKCSGDGKV
                     LLTIGIPNKPAPFMSGEPFHRCTHTALSPKGEIYVSDGYGNACVHKFTPDGRLIKTWG
                     EPGTDPGQFNIVHNIATDADGYVYVADRENHRVQVFDGNGKYETQWNNLHRPCALYCC
                     GGKQPNFVIGELGPGMPVNRKVPNLGPRLTIVDAKGKRIARLGGENGPGLEAGKFLAP
                     HGIALDSRGDIYVGEVGVTDWKTSFPDTPMPTEVGVVRCLQKLEKV"
     gene            complement(5268..>5649)
                     /locus_tag="VN968_07505"
     CDS             complement(5268..>5649)
                     /locus_tag="VN968_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HXL29121.1"
                     /translation="QFSDIESFVAEGARMKSGETLAADLIVLATGYKGQQHLVRKLFG
                     DEVAGRVGPIWGFGEGQELRNMYTRTAQPGLWFIAGSLAQCRINSKYLALQLKAIEEG
                     LLPRGALSSPLPLCERVPSQSEGG"
BASE COUNT          965 a         1738 c         1829 g         1117 t
ORIGIN      
        1 agcgtggtca ccgaggagag cgcgaagtaa gccgtaaaac cattgcgcgg ggaatgccgg
       61 gtcgttccgg tgtgaccgtg gtgactacgc tcgtacgctt tttattttgc gtgcgaggct
      121 gcgggtgcat cgagcgcccg gcattccctg cgccctctgt tttctaaggg cggaacgttc
      181 ttgcaaacct cggacgcatc gcgccgcgag atcgcggagt tgtatctgga attcacgtca
      241 ttgcgagcgt agcgaagcaa tccatttcgc cgcgaaggaa gctggattgc ttcgcttcgc
      301 tcgcaatgac gacttaagaa aatcctctcg gctgtttgaa aatcgaatcg gaaacggcac
      361 tggagcgagc cttgcgagag cggcccgcgc gccggccttt acggatcgag ctcggtatcc
      421 cagtacaaat aatcgagcca gctgtcgtgc agatagttcg gcgggaacag ccggccgttg
      481 cgatgcagct gatgcaccgt cggggcgaac gggaactgcc ttggaaacat ccgcgcctgc
      541 tgcggcgtca ggttgccctt gcgcagattg cacggcgaac acgcggcgac cacgttttcc
      601 caggtggtat ggccgccctt gctgcgcggg atgatgtgat cgaaggtgag atcgtcgttg
      661 gaggagcaat actggcagac gaagcggtcg cgcaggaaca cgttgaaccg ggtgaaggcg
      721 ggatgcgtgc tgggcttgac gaaggatttc agcgacacca cgctcggcag ctggatctcg
      781 aacgaggggc tgcgcaccgc gcggtcatat tgctcgacga tattgacgcg atcgagaaac
      841 accgccttga tcgcgtcctg ccacgaccag agagacagcg ggtaataact cagcggccgg
      901 aagtccgcat tcagtaccag agccggccaa ccgccttgcg agacatgtgc gttcaagtaa
      961 cgctcctggc ccccatatta gctgcgaagc agcttgcgtt gacatactac aggcagcgtg
     1021 acgggattgt gaagagcatt ggcggctttt atccgccgcc gcgcggggtc gcttccggat
     1081 tgcacgatcg gcgcaaacac cgttaaacga ggcggacggc accttttggc cgcatttgga
     1141 cgcagcaaga tgctcaccac gtcgcgctat ctcgaggcgc cgcgccggct gcgggccttc
     1201 gtgcgcgcgc atgaaacgag tctggtggtc cttgcggcgc tggtcggaac catcggcggg
     1261 tgggtcgtgc tggcgatgag ccttgcggtt gccgcccttc atgccgtcct gttcaacatc
     1321 gatatcaggg accgtctttc gagccagctc ggcatcgacc cgctgcgcgc cgtgctggtt
     1381 ccgagcctgg gtgggctggt gctgggattg gccttgctcc tgctgcagcg atggcggccg
     1441 gcgcgcgaga tcgacccgat cgaggccaat gccctgcatg gcgggcgcat gtcgttccgc
     1501 ggcagcgtga tcgtggcatt gcaaacggtc tggtccagcg gcgtcggtgc ttcggtggga
     1561 ctcgaggccg gctacaccca gcttgccagc ggcatctccg cttccctggg ccgcgccttt
     1621 catctgcgcc gcgccgatca gcgcatcatg gtcggctgcg gcgccgcggc cgcgatcgcg
     1681 ggcgcgttcg gagcgccgct cgccggcgcc ttctatgcct ttgagctggt gatcggaggc
     1741 tataccccgg ccagcctgac ggcggtcgga gtggcggcgg tcgcgggcta ttttgtcacc
     1801 catggatttg ccgcgctgtc gcttggcatt ggcgtcggtc cggtcggcga cgtgctcggg
     1861 cgcgatctcg cccttgcggg gctgcttggc attctggcgg cgctgtttgg catcgccatc
     1921 atgcgcggcg tggcgctgtg cgagtcgttg ctgaccgggt ccggaatctg gctgccgctc
     1981 cgcccggcgc ttggcggtct tgccgtcggt ctaatggcat tgctgacgcc gcatgtgatg
     2041 tcgtcggggc acggggctct gcattttacc agcatcgttt cgatgccgtt gacgatgatt
     2101 gccggtgtgt tcgtactgaa agcgctggcg tcggtgatat cgcttggcag cggctttcgc
     2161 ggcggattgt ttttcgccgc gctgtttctg ggcgcgctcg gcggccgcct gttcgcggcg
     2221 ggcattgaca tgatctggcc gggcctcaac ctcgatccca acgtctatgc gatcgtcggc
     2281 atgagtgcgc tgtcggcctc ggtgatcggc ggaccgctga cgatggcgtt catcgcgctg
     2341 gagtccaccg gcaatctctg gctcaccacc gcggtgctgg tcgcggtcat catctcgacc
     2401 cagatcaccc gggaattatt cggttactcg ttcgcgacct ggcgattcca cttgcgcggc
     2461 gagaccatcc gcagcgccgc cgatgtcggc tggatccgcg atctcacggt gcggagcctg
     2521 atgcggccgg acgttcccac cgtaaacgcc gacatgccca ttggacaatt tcgcggcagg
     2581 tttccgctgg gatcgaagac ccaggtggtg gcggtcgatg ccgccggccg ctacgccggg
     2641 ctggcactgg tggcggacgc gcatgcgccg ggtctcgacg cgaagagcgg ccttaacggc
     2701 atccttcatc atcgcgatgt cgtgctgcac cccgtcatga acatccagga agcgatcgag
     2761 gtctttgatg ccgcggaagc agaatcgctt gttgtcgtcg aaagcggcga cgaacaccgg
     2821 ccgatcggaa tcctcaccga agcccacgcg atgcggcgct atgcggaaga atccgaacaa
     2881 cggcggcgcg aggcggtcgg cgatgtctga gcggcacggc agagaagggc agaaagaatt
     2941 acgccagagc atgatgagtt taggttgaag cggcggacca cttctccctc gccccgctct
     3001 tgcggggaga gggtcggggt gaggggctct ctcggcgcga ccggactcgc ggatagtctc
     3061 cctcacccgg attgctccgg acgatgcttt gcatcgctcg tagcaatccg acctctcccc
     3121 gcaagcgggg cgaggtgagc caacatccgc gccgattcaa ctcaaagcca tcacgcccta
     3181 aacatgtatc ggtgcgctca attcgggagg agccccatgg gccgactgct gacggttttg
     3241 gtcgcgacgc tgcttggttc tggatcgatc gacatggccg ccgcccaaaa ctatccggcg
     3301 cgcccgattg ccatgatcgt gccgttcccc gccggcggcg cgaccgacac gctggcccga
     3361 ttcctgagcg agaaaatgcg gggaattctc ggtcaggccc tgatcatcga gaacgtggcc
     3421 ggagccgccg gcagcatcgg cgttggccgc gccgttcgtt cacccgccga tggttacacg
     3481 ctgagcatcg gcacctccac cacgcacatg ctgaccggcg gcctctacgc gcttcagttc
     3541 gatctgttga gagatctgga gccaatcatt ctgatcggca gcgagccgct tttgatcgtg
     3601 ggcaggaaga gcctgccggc agacgatctg aagggactga ttgcgtatct caaggccaat
     3661 cccgacaagg cgtcggttgg catcgccggt gtcggcgcga ccggtcatct cacgggtatt
     3721 tccttccaga aagcaaccgg aaccaaattt caattcgtgc cgtatcgcgg caatgccccg
     3781 gccatgcagg acctgctggc ggagcagatc gatttcatga tcgagccatc gtcgaacttc
     3841 aagtcgctgg tcggcgccgg aagcgtcaag ccttatgcga tcacgggcag ggcgcggctg
     3901 gcttcgtcac cggatattcc gaccgcggac gaggccggtc tgcccggctt ccttgcttcg
     3961 ctctggtacg gcctttgggt acccagggat acgccaaagg acatcatcgc caggctgaat
     4021 gcgacgatgg tgcaagtgct cgccgatccc tcggtgaaag agcgttttgc ggagcttggc
     4081 atccagatcg cgccgctcgc gcagcaatcg cccgaagcgt tgcgtgcatt ccagaaagcc
     4141 gaggcggaac gctggtggcc gatcatcaag gcggcgaacc tcaaggcgga atgaaagtgt
     4201 tacaccttct ccagcttctg caggcagcgg accacgccga cctccgtcgg catcggcgta
     4261 tccgggaaac tcgtcttcca gtcggtgacg ccgacttcgc cgacatagat gtcgcccctg
     4321 gaatccagcg cgatgccgtg cggggccagg aatttgccgg cttcgagtcc cggaccattt
     4381 tccccgccga gccgcgcgat acgctttccc ttggcgtcga cgatggtcag gcgcggaccc
     4441 aaattcggca ccttgcgatt gaccggcatg cccggaccga gctcgccaat aacaaaattc
     4501 ggctgcttgc cgccgcagca gtaaagcgcg cagggccggt gcaaattatt ccactgcgtc
     4561 tcgtacttgc cgttgccgtc gaacacctgg acgcgatggt tctcgcggtc ggcgacatag
     4621 acgtagccgt cggcgtcggt tgcgatgttg tggacgatat tgaactgccc gggatcggtg
     4681 cccggctcgc cccaggtttt gatcagtctg ccgtcgggcg tgaatttgtg gacgcaggca
     4741 ttgccgtacc cgtccgacac gtagatttcg cccttgggcg agagcgcggt gtgggtgcag
     4801 cgatgaaacg gctcgccgct catgaagggc gcgggtttgt tcgggatgcc gatcgtgagc
     4861 agcaccttgc cgtcgccaga gcacttgcgc accgtatggt cgccgtcgtc ggtgcaatag
     4921 agattgtcct cggtatcgat gtgcagccca tgcgcgcggt tgaacaggcc ttcgccccag
     4981 ctggtgatga aattgccttc gcggtcgagc accaccatcg gatgggcgcc gcgattgaag
     5041 acgtagacac ggtccttgct gtcgaccgcg accgaggcga catcagagag attccacccc
     5101 tccggcagct tcgcccaatt ctccaccacg cggtagcggt gttcgcccga gccgagaatg
     5161 accggcatgt gtgcttcctt tcttccttct ccccttgtgg gagaaggtgc actcgatgcg
     5221 aagcatcgag ggcggatgag gggtctgcat ccgcggagac agatccctca cccgccttcg
     5281 ctttgcgaag gcaccctctc gcacaagggg agaggggaag aaagcgcgcc gcgcggcagc
     5341 aggccttcct cgatcgcctt gagctgcagc gccagatatt tcgagttgat ccggcactgc
     5401 gccaggctgc cggcgatgaa ccacagcccc ggctgcgcgg tgcgggtgta catgttgcgc
     5461 agttcctgac cctcgccgaa accccagatc gggccgacgc ggccggcgac ttcatcgccg
     5521 aacagttttc gcaccagatg ctgttggccc ttgtagccgg tcgccagcac gatcaggtcg
     5581 gcggcaagcg tctcgccgct cttcatccgg gcgccttcgg cgacaaagga ttcgatatcc
     5641 gaaaattgc
//
LOCUS       DATNOI010000196         2306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1011110,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000196 DATNOI010000000
VERSION     DATNOI010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011110"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VN968_07510"
     CDS             <1..274
                     /locus_tag="VN968_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HXL29122.1"
                     /translation="PADVIERALADKNREMLLVLAKALDFSWQTTMSLLFLGAKDHRI
                     TAQDLNDLEREFGRLNLETSRSVLKFYQTRKHAAATESRAEAAARA"
     gene            320..547
                     /locus_tag="VN968_07515"
     CDS             320..547
                     /locus_tag="VN968_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29123.1"
                     /translation="MNAIPSGAVAGERLGTATDEPNSDKSSDAAWIVLEAANDLGDHA
                     TVEACRRVIDANLNGTPPSQSDLLIVLDYFR"
     gene            867..1508
                     /locus_tag="VN968_07520"
     CDS             867..1508
                     /locus_tag="VN968_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXL29124.1"
                     /translation="MIEAVIWDFGGVFTTSPFEAFARFETERGLPAGIIRRTNASNHL
                     ENAWAKFERAEVDTEAFDRLFAAESLALGAEVRGKDVLPLLVGDLRPDMVEAFKTIAA
                     KLKTGCITNNLPANAIGSLGGRSIYVAEVMALFDHVIESAKIGLRKPDPRIYRMMVQA
                     LHVDPKRCVYLDDLGVNLKPAREMGMTTIKVVNAPQAIAELEKATGLVLRLPP"
     gene            complement(1516..1695)
                     /locus_tag="VN968_07525"
     CDS             complement(1516..1695)
                     /locus_tag="VN968_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HXL29125.1"
                     /translation="MSDEPYKSWQCRTCGYIYDEAAGAPDEGLAPGTRWADIPDDWVC
                     PECGTPKSDFDMVEL"
     gene            complement(1799..>2306)
                     /locus_tag="VN968_07530"
     CDS             complement(1799..>2306)
                     /locus_tag="VN968_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL29126.1"
                     /translation="AGAAWQGRIGEVDEAVLRQSFELNFYGHQRVAQAAVKIMRAQGT
                     GGCLLFNVSKQAVNPGPDFGPYGLPKAATLFLVRQYALDYGSEGIRANAVNADRIRSG
                     LLTEDMIASRSKARGLSEQAYMRGNLLGREVAAEDVAQAFLAQALALKTTADVTTVDG
                     GNIAAALR"
BASE COUNT          465 a          694 c          708 g          439 t
ORIGIN      
        1 gccggccgac gtgatcgaac gcgccttggc ggacaagaac agggaaatgc tgctggtgct
       61 ggccaaagcc ctcgactttt cgtggcagac cacaatgtca ttgctgtttc ttggcgccaa
      121 ggatcaccgc atcaccgcgc aggacttgaa cgacctggaa agggagtttg gccgcctcaa
      181 cctcgagaca tcgcggagcg ttctgaaatt ctatcagaca cgcaagcatg ccgcggctac
      241 cgagtcccga gccgaggcgg ctgcccgcgc ttgatatgga ctgagattat cgatctggaa
      301 tggcagggaa gtatcgcaaa tgaatgcaat cccatccggc gcagttgcag gcgagcgcct
      361 cggcacggct accgatgaac cgaactccga caagtcctca gacgcggcct ggatcgttct
      421 ggaggcagcg aacgatctcg gagaccatgc gaccgtcgag gcctgtcgcc gtgtcatcga
      481 cgcgaacctg aacggcacgc ccccatcgca gtccgatttg cttatcgtgt tggattactt
      541 ccggtaaata tagaccggtc ttggctgact caaaaagcga ccaatgaaac tcgtcatgcc
      601 cggccttgcc gccttcgcta aagctacggc ggcctagcac cgaaaaagcc cgtcgaagcc
      661 tcggcgtaga cgggtgccgg gcatccacgt cttagctgca tttaagcaag aaagacgtgg
      721 atggccggga cgaagcccgg ccatgacgaa aacgaggggg cgcaaacatc ggcgcctaaa
      781 atcggacttg cccgcctcaa ggctggtgcc ggcccggacg tcgtgttagc tgcttcgtaa
      841 atcgcgctgg cattggcagg aaatggatga tcgaggcagt gatctgggat ttcggcgggg
      901 tgttcactac ttcgcctttc gaggcttttg cgcgtttcga gaccgaacgc gggctgcccg
      961 ccggcattat caggcgcacc aatgccagca atcatctgga aaatgcctgg gcgaaattcg
     1021 agcgcgccga agtggatact gaagcattcg atcgattgtt cgcggcggaa tcgctggcgc
     1081 tcggcgcgga agtgagaggc aaggacgtct tgccgcttct ggtcggggat ctgcggcccg
     1141 acatggtcga ggcgttcaaa actatcgcgg ccaaactcaa gaccgggtgc atcaccaaca
     1201 accttcccgc caacgcaatc ggtagcctgg gcggccgatc gatctatgtc gccgaggtga
     1261 tggcactgtt cgatcacgtc atcgagtcgg caaaaatcgg tttaaggaag cctgatccgc
     1321 gcatctaccg gatgatggtg caagcacttc acgtcgatcc caagcgttgc gtctatctcg
     1381 acgatctcgg cgtcaacctg aagccggcgc gggaaatggg catgaccacc atcaaggttg
     1441 tcaatgcgcc gcaagccatc gccgagctcg agaaggcgac agggttggta ctgcgtttgc
     1501 ctccgtaact cgcatctaca gctccaccat gtcgaagtcg ctcttgggcg tgccgcattc
     1561 aggacaaacc cagtcgtctg gaatgtcggc ccaccgggtg cccggagcca ggccttcgtc
     1621 cggcgcgccg gctgcttcgt catagatgta gccgcaagtc cggcactgcc aggatttgta
     1681 gggctcgtca ctcattggat tctcggtctt gcatggttcg tgcccgggac gtgtcacgag
     1741 tgtagtgggg agatttcgcg aagaccagag ggctgcgccg cagcaacttc cactccagtc
     1801 agcgcagcgc ggcggcgatg ttgccgccgt cgacggtggt gacgtcggcg gtggttttca
     1861 gcgccagcgc ctgggccagg aatgcctgcg cgacgtcctc ggccgcgact tcccggccga
     1921 gcagattgcc gcgcatatag gcctgctcgc tgagcccgcg ggcttttgag cgtgacgcga
     1981 tcatgtcttc ggtcaggagg ccggaacgga tgcggtcggc attgaccgca ttggcgcgaa
     2041 tgccttcgct gccgtagtcg agcgcgtatt gccgcaccaa gaacagcgtc gccgccttcg
     2101 gcagaccata ggggccgaag tcgggtccgg gattgaccgc ctgtttggaa acgttgaaca
     2161 gcagacagcc gccggtgccc tgcgcccgca tgatcttgac ggcggcctgc gccacgcgct
     2221 ggtgaccata aaaattcaat tcaaaactct gccgcaatac cgcctcgtcg acctcaccga
     2281 tcctcccctg ccaggcggcg ccggca
//
LOCUS       DATNOI010000197         6339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1130969,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000197 DATNOI010000000
VERSION     DATNOI010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130969"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VN968_07535"
     CDS             <1..339
                     /locus_tag="VN968_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="family 3 adenylate cyclase"
                     /protein_id="HXL29127.1"
                     /translation="DATVEEMGSFNDLQRISASAENAARLLQSSGDVNVLDLLPGIVA
                     PTLVLHCREDAAMPFAQGRLIASRIPRARLVPLESRNHVLLPRDPAWAAFVSEARRFL
                     GEGVPTMSGA"
     gene            601..1023
                     /locus_tag="VN968_07540"
     CDS             601..1023
                     /locus_tag="VN968_07540"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HXL29128.1"
                     /translation="MMQDDDNSFGRAVERVRTALAAMGFGNPEPYIDCWAKSDDATLF
                     GAWGPIERGYARLTETFRWVGSRFKSGALVPQDIVCFESGDLAYTVGFEVGEVVVDRG
                     PATPMTIRVTHIYRRIDGEWRLVHRHADFPPADQRAPA"
     gene            complement(1020..2045)
                     /locus_tag="VN968_07545"
     CDS             complement(1020..2045)
                     /locus_tag="VN968_07545"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HXL29129.1"
                     /translation="MTALRIRARDLMTEQELIEVRTRSTWKSVALIAHAWTLILGSIA
                     MVAVWPNPLTFVLAVAIIGSRQLGLSILMHDGAHGCLANGERLNMALSQWLCAYPVFA
                     ETKAYRRYHLQHHARTQQHDDPDLILSAPFPITRPSYRRKFWRDISGQTGYAQRKAQF
                     LNALGDQSWPLAQRAAHFWDRLGPQIVFNLAFLAGLAAAGVWWAYPLLWLLPLVTWMM
                     VITRIRNIAEHAVVPDSDDPLRNTRTTRAGFLERLFIAPYYVNYHLEHHLLFYVPCYN
                     LPRVHEILSRSPHAARMEVQPNYAAVLRLATAKPANEDRPGQLVSNVRRARAGIALDN
                     NQISSGF"
     gene            complement(2042..2674)
                     /locus_tag="VN968_07550"
     CDS             complement(2042..2674)
                     /locus_tag="VN968_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HXL29130.1"
                     /translation="MRSRLLDAASRLFKERGLAAISIADIAAAVDAFPSQITYYFRTK
                     EALFVEAACRDVLYVARAAEQHAGKQLSPADYARALVDSVMGSDAVGFFVEAMTLTRR
                     RQDLAPLVERTVERLHAEGLRAYAAEMAAHGWHSRFDPETSARRFWTIAIGVTLEGQA
                     MGRSPDDLVAEMLRLLGPQADVPAAAARLAVVRTGNSAPDSLSADKEKQP"
     gene            complement(3121..3729)
                     /locus_tag="VN968_07555"
     CDS             complement(3121..3729)
                     /locus_tag="VN968_07555"
                     /inference="COORDINATES: protein motif:HMM:NF044451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL29131.1"
                     /translation="MRENKPERSRREEYTEATRQALLSTGRDIFASEGYQAAGIEAIS
                     RAARVTRGAFYHHFEDKKALFDAVVVSMQAEAAARIGERARAQRKMWDRLSVGIDAYL
                     DACLEPAYARIVIQEAPAVLGNKRYREIEEAYPMALLTATLNALKREGELDFEDIDLL
                     SRMVDAMICKVALMLPEADHPKKLRERGQKIIGSLLAVFRRT"
     gene            3891..4451
                     /locus_tag="VN968_07560"
     CDS             3891..4451
                     /locus_tag="VN968_07560"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HXL29132.1"
                     /translation="MVLASEDRMNSHRMLELARALAIAKSRQDVPAAMKLFHQDMLLE
                     NPAFGTTARGLAANEKALTSFFTSFPDYNVVLQGHAGNDDTLMCWGTVRMTMTGDRFG
                     VVPNGRPAELPVFIQFVFKDDLIAGERFFFDLSALCAQSGVSTDAVRRKLFGEAAASQ
                     IAADQNPPAGASRNAASACSPGIEGQ"
     gene            4497..4949
                     /locus_tag="VN968_07565"
     CDS             4497..4949
                     /locus_tag="VN968_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HXL29133.1"
                     /translation="MAHLFDIVIDLNPRLNIGGGPFGQRILFGAAGGSFEGPRLRGEV
                     LAGGGDWALFRPDGAMMLDVRLTLRTHDDALVHMTYGGRWITPPELRADMADPVKRYQ
                     VDPASYYFRTNPLFETGAKQYAWLNDIVCVGSGYLIEGGIAYKVSQVA"
     gene            5011..5712
                     /locus_tag="VN968_07570"
     CDS             5011..5712
                     /locus_tag="VN968_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HXL29134.1"
                     /translation="MSLQRRLVSEALGTSFLLIAVVGSGILGERLAAGNTAIALLANA
                     LATGAALFALIEWLAPLSGAHFNPLVTLAMAFRGDITRRAAAAYVPIQLIGAVIGVGV
                     ANAMFDAPVFSWSTHARHGPSQWLGEFVSTFGLVGAVWTCSRMRPSSVAGVVAAYIGG
                     AFWFTPTDFANPAVTLARALTDTFAGIRPSDVPGFIIAEIAGAAAAVVMFRWLLPNAC
                     TGVSEVNPTASLGKR"
     gene            complement(5846..>6339)
                     /locus_tag="VN968_07575"
     CDS             complement(5846..>6339)
                     /locus_tag="VN968_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXL29135.1"
                     /translation="SPLNVTTTGTPTIPAPTNVKIDAVGAASVTLSWTAPADATGFDV
                     FRAGTSGGPFAKVNPSLVTGTSFTDTGLTGSTTYFYVIKSEGAGGGLSAPSMEVSAKT
                     EAPPTCFTATNFDHVVAGRAHDNFFFAEANGSNQLLGLDNVFIVTTLKQTGPNSYVIA
                     SCP"
BASE COUNT         1138 a         1956 c         2036 g         1209 t
ORIGIN      
        1 gatgccacgg tcgaagagat gggctcgttc aatgacctgc agcgcatctc ggcctcggcc
       61 gagaatgccg cccggctgct gcagtcgagt ggagacgtca atgtcctcga ccttctgccg
      121 ggcatcgtcg cgccgacact cgtcctgcat tgccgcgagg acgccgccat gccattcgcc
      181 caaggtcggc tcatcgccag ccgcattccg cgggccaggc tcgttccgct cgagagccgc
      241 aaccacgttc tcttgccgcg cgatccggcc tgggccgctt tcgtcagcga ggcacgccgg
      301 tttcttggcg aaggcgtgcc gacgatgtct ggcgcatagc gaaccggcac tacggtggcc
      361 gataattcca gcgcccgcta atgtgccgag cgcttcagtt tcatcgtccg gatttcgccc
      421 ggttcgcctg ctacggcgac acgagtggct ccttcatcga agtgtttccg tggggccacg
      481 tcctcgatcg ggttgccccg tcgagcgcga gctttcgaca tgccgcagcg gccgggggcc
      541 cgcattcttg caagcccgtc gcgttccggt ggcgcgccag cgattgagat gagacattcc
      601 atgatgcaag acgatgacaa tagtttcggc cgcgcagttg agcgagtgcg cacagcgctc
      661 gccgcgatgg ggttcggcaa tcccgagccc tatatcgact gctgggcgaa gtctgacgac
      721 gccacgctgt tcggagcgtg gggtccgatc gaaaggggtt atgcgcggct caccgagacg
      781 tttcgttggg tcggcagccg cttcaagagt ggagcgttag tgccgcagga catcgtctgc
      841 ttcgagagcg gcgatctggc ttacaccgtc ggcttcgagg ttggcgaagt cgtcgtggac
      901 cgcggcccag cgacgccgat gacgattcgc gtcacccata tctatcgacg aatcgacggc
      961 gagtggcgtc tcgttcaccg ccacgccgat ttcccgccgg cagatcaacg ggcgccagcc
     1021 taaaacccgg acgagatttg attgttgtcg agagctatgc cggcccgggc acgccgaacg
     1081 ttactcacga gttggcccgg ccggtcctca tttgcaggct tcgcggtcgc aagccgcagc
     1141 acggctgcat agttcggctg cacctccatc cgtgcagcat gagggctgcg agagaggatc
     1201 tcgtgaacgc gcggcaggtt gtagcagggg acatagaaca gcagatgatg ctcgagatga
     1261 tagttgacgt aatagggcgc aatgaacagt cgctcgagaa atcccgcgcg cgtggtgcgc
     1321 gtgttgcgca acggatcgtc gctatccggc accacggcat gttcggcgat gttgcggatg
     1381 cgggtaatga ccatcatcca ggtcacgagc ggtaacagcc acaacagcgg ataggcccac
     1441 cacacgcccg ctgcggcaag tcctgccaag aacgccagat tgaagacgat ctgcggtccc
     1501 agcctgtccc agaagtgcgc ggcccgctgc gcgaggggcc acgattggtc gccgagcgca
     1561 ttgagaaact gcgccttgcg ctgcgcataa ccggtctggc cgctgatgtc gcgccagaat
     1621 ttccggcgat agctaggccg ggtgattgga aagggcgccg aaaggatcag atcaggatcg
     1681 tcgtgctgtt gggtgcgtgc atgatgctgc agatgatagc ggcgataggc cttggtctcg
     1741 gcgaacacgg ggtaggcgca gagccattgg ctgagagcca tgttcaacct ctcgccgttc
     1801 gccagacaac cgtgcgcgcc gtcatgcatc aggatcgaaa gtccgagctg acgtgagccg
     1861 atgatcgcaa ccgccagcac gaaggtgagg gggttgggcc acaccgcaac catcgcgatc
     1921 gaaccgagaa tcagcgtcca tgcatgcgcg atcagggcaa cactcttcca ggtcgaccgc
     1981 gtgcgcacct cgataagctc ttgctcggtc atcaagtcgc gcgcgcgaat gcgaagtgcg
     2041 gtcatggctg tttctccttg tcggcggaca agctgtcggg agcggaattt cctgttctaa
     2101 cgaccgcgag ccgcgctgcc gcggcaggca catcggcctg cgggccgagc aggcgcagca
     2161 tctcggcgac aagatcgtcg ggtgagcggc ccatcgcctg cccttccagc gtgacgccga
     2221 tcgcgatcgt ccagaagcgc cgcgcactgg tttccggatc gaaccgcgaa tgccagccgt
     2281 gcgcggccat ctcggccgca taggcgcgca gaccttcggc atgcagccgt tcaaccgtcc
     2341 gctccaccag cggcgcgaga tcctggcgac ggcgggtcag cgtcatcgct tcgacgaaga
     2401 agccaaccgc atccgacccc atcacgctgt cgaccagtgc gcgggcatag tctgccggcg
     2461 agagttgttt tccggcgtgc tgctcggccg cgcgcgcgac atagagcacg tcacggcatg
     2521 ccgcctcgac gaacagcgct tccttggtgc gaaaataata ggtgatctgg ctcggaaagg
     2581 cgtcgaccgc tgcggcgata tcggcgatcg aaatcgccgc cagcccgcgc tctttgaaaa
     2641 ggcggctcgc cgcatcgagc agacgcgagc gcatttggcg gcccgccgta cgggtagccc
     2701 gtgcggtgtg cttggggtcg cctaaacgct cttcggtcga agcgcctttc atacgtcctc
     2761 gtcgctgcgc aaacgctgtg gtagccagct gttttttagt ttgtatgtca tacaaacaaa
     2821 cccgtcaagt ggccttggag ggggctggcc gcaagccagg aaggagggat ggagcgaagg
     2881 tgaccgtcgc cttgctggcg gcgaactgcg gcactagtcc ggccgctacg cggaagcaca
     2941 tgtcgcgttc gagcggcggc aggggggcaa caagcacgac cgcgatctgc cgaggcagcg
     3001 ccaccgaacg gtcggcgccg cgatgtcgtc atgatcccgg cgcgcgccga tgatctcttc
     3061 cggtccggca actcagccta tagcagacgc cgccacggtg acttatggca ctcgcaagtc
     3121 tcaagtccgg cggaagactg ccagcagact gccgatgatt ttctgtccac gctctctgag
     3181 tttcttcggg tggtcggcct ccggcagcat cagcgccacc ttgcagatca tggcgtcaac
     3241 catgcggctg aggaggtcga tgtcctcaaa atcaagctcg ccctcgcgct tcagggcgtt
     3301 cagggtcgcg gtgagaaggg ccattggata ggcttcctcg atttcccgat agcgcttgtt
     3361 gccaagcacc gccggcgcct cctggataac gatacgcgcg taagctggct caaggcaggc
     3421 gtccaggtag gcgtcgattc ccacgctcaa acgatcccac atcttccgct gggcccgggc
     3481 ccgttctccg atcctggccg ccgcctccgc ctgcatggaa acgacaacgg catcgaacag
     3541 ggccttctta tcctcgaagt ggtgatagaa ggcgccccgc gtcacccgcg ccgcccgcga
     3601 gatcgcctcg attccggccg cctggtagcc ctcgctcgca aagatgtccc ggccggtcga
     3661 cagcagcgct tgtcgggtgg cctccgtgta ctcctcacgg cgacttctct caggtttgtt
     3721 ctctcgcatg caggcaacat accgcttgac gaaaattgtt catatgatat gtaacataca
     3781 ttagttatgt taaaaacatg acgaatgccc tgcgaagaag atgatcgggg ggccacttgg
     3841 ggcagcaccc ggaacgcgca gccggccatt ttcatggcgg agaagcgtgt gtggtgctgg
     3901 catcggagga tcggatgaat agccatcgaa tgctcgaact ggcgcgtgca cttgccattg
     3961 ccaagagccg gcaggatgtc cctgccgcca tgaagctttt ccaccaggac atgctgcttg
     4021 agaacccggc cttcgggaca acggcgcgag gccttgccgc gaatgagaaa gcgctgacca
     4081 gcttcttcac ttccttcccg gactacaacg tcgtcctgca aggtcacgcc ggcaatgacg
     4141 acactttgat gtgctggggg acggtgcgga tgaccatgac cggcgaccgg tttggcgtgg
     4201 tccccaacgg caggccggcc gagctaccgg tgttcattca gtttgtcttc aaggacgacc
     4261 tgatcgccgg cgagcggttc ttcttcgacc tgtcggcgct ctgtgcccag tcaggcgtct
     4321 cgacggacgc ggtccggcga aaactgttcg gagaggcggc ggcttcgcag atcgcggccg
     4381 accagaaccc accggcaggt gcctcgcgga acgcagcatc tgcctgctcc ccagggattg
     4441 aagggcaata acccatgaca tcagacccac gcgtgaaggc cgttggcgag atcaagatgg
     4501 cgcacctgtt cgacatcgtc atcgatttga atccgcggct gaatattggc ggtggcccgt
     4561 tcggtcagcg gattcttttc ggcgctgccg gcggatcgtt cgaagggccg cggctgcgcg
     4621 gcgaggtgct ggcgggtggc ggcgactggg cgttgtttcg tcccgacggc gcgatgatgc
     4681 tggacgtccg attgaccctg cgcacgcacg acgacgccct ggtgcacatg acctacggtg
     4741 gccgctggat tacgccgccc gaactgcgcg ccgacatggc cgatcccgtc aagcgatatc
     4801 aggtcgatcc tgccagctac tatttccgga ccaaccctct gttcgaaacc ggggcgaagc
     4861 aatacgcctg gctgaacgac atcgtctgcg tagggtcggg ttacctgatc gaaggtggca
     4921 ttgcctacaa agtctctcag gttgcctgac agcgaggctg cggcgaccga agtggcagat
     4981 ccgggcgatg cgcgcgttgc agtcccagcg gtgtcgctgc aacgtcgact ggtgtcggag
     5041 gcgctcggca ccagcttcct gctgatcgcg gtggttggct ccggaatcct gggagagcgg
     5101 ctcgctgccg ggaacactgc cattgcgctg ctcgccaacg ccttggctac cggggcggcg
     5161 ctgttcgccc tgatcgagtg gctggcgccg ttgtcgggtg cacatttcaa tccgctggtc
     5221 acacttgcca tggctttccg tggcgatatc acgcgccgcg ccgctgcggc ctatgtgccg
     5281 atccaactca ttggcgctgt catcggcgtc ggagtcgcaa atgcaatgtt cgatgcgccg
     5341 gtgttttcct ggtccacgca tgcgagacac ggcccttcgc aatggcttgg cgaattcgtg
     5401 tccacgttcg ggctggtggg cgcggtctgg acgtgttcgc ggatgcgccc gtcgtcggtg
     5461 gctggggtgg ttgccgccta tatcggcggt gcattctggt ttacgcctac ggacttcgcg
     5521 aacccggcgg tgacccttgc gcgagccctc acggacacat tcgccgggat taggccttca
     5581 gacgttcctg gcttcatcat cgcggaaatc gcgggcgcag cagccgcggt cgtgatgttc
     5641 cggtggctgc tgccgaacgc atgcacaggt gtcagcgaag taaatccgac ggcaagcttg
     5701 ggtaagcggt aggttcgcgc agttgttaaa ggaaacgcgc gccgcgttcg cagacgcgat
     5761 gtcgatgatc gcgcggggct gaaatcaaag ccctatgcat tacggccgcg tgcaaaaccg
     5821 tacgcggcgt gtcggcgcct tggcgtcaag ggcaggaggc gatcacgtaa gaattcgggc
     5881 cggtctgctt cagcgtggtg acaatgaaca cgttgtcgag gcccagcaat tggttcgagc
     5941 cgttggcctc ggcgaaaaaa aagttgtcgt gcgcgcgacc ggccaccacg tggtcaaaat
     6001 tcgttgccgt gaagcaggtc ggcggggcct cggtcttggc gctgacttcc atagagggag
     6061 cgctcagtcc accacccgca ccctcggatt tgatgacata gaaatatgtc gtgctccctg
     6121 tcagcccggt atcggtgaaa ctcgtcccgg taaccaggct cggattgacc tttgcgaagg
     6181 gaccccccga tgtccccgcg cggaacacgt cgaagccggt cgcgtccgcc ggcgccgtcc
     6241 aggacagcgt caccgatgca gcgccgaccg cgtcgatctt cacgttggtc ggagccggga
     6301 tcgtaggcgt tccggtggtc gtgacattga gcggactag
//
LOCUS       DATNOI010000198         6921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1502490,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000198 DATNOI010000000
VERSION     DATNOI010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502490"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VN968_07580"
     CDS             complement(<1..572)
                     /locus_tag="VN968_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXL29136.1"
                     /translation="MSGLTFTEDAARHLARVYLTPDVVAQRAETMKQLALSPGERVLD
                     IGCGPGFLCESIAAAVGSDGAVTGIDISSDLIALCKTRNPPKWLSYAVGDATQIAQPD
                     ASFDVVVCTQVAEYVPDVDRVLAEAFRVLKPGGRAVIVATDWDAVVWHSEKPERMAAV
                     MKSWESHCAHPRLPRSLSNRFVRAGFRVDGA"
     gene            complement(769..2811)
                     /gene="thiC"
                     /locus_tag="VN968_07585"
     CDS             complement(769..2811)
                     /gene="thiC"
                     /locus_tag="VN968_07585"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HXL29137.1"
                     /translation="MNKPVSSAELKPAVTTGPLPSSRKIFVTPDEAPDVRVPLREIML
                     SEGAGEPNLSVYDTSGPYTDPAVTIDVNKGLPRNRTAWVLERGGVEAYEGRAVKPVDN
                     GNVSADHAAKAFDAFHKPLRGLDAASVPSHLAGSEASEARSRGQGGGSTRAVVRGATP
                     TPNPSPQGGGEQKPPMITQLEFARAGIITKEMIYVAMRENLGRKVQHERAEAALADGE
                     SFGANIPAFITPEFVRSEIARGRAIIPSNINHGELEPMIIGRNFLTKINANIGNSAVT
                     SSVEEEVDKMVWAIRWGADTVMDLSTGRNIHTTREWILRNAPIPIGTVPIYQALEKVN
                     GDPVKLDWEVYKDTLIEQCEQGVDYFTIHAGVRLPYIHLTANRVTGIVSRGGSIMAKW
                     CLAHHKESFLYTRFDEICDLMRKYDVSFSLGDGLRPGSIADANDRAQFAELETLGELT
                     KIAWDKGCQVMIEGPGHIPMHKIKINVDKQLQECGEAPFYTLGPLTTDIAPGYDHITS
                     GIGAAMIGWFGCAMLCYVTPKEHLGLPDRNDVKTGVITYKIAAHASDLAKGHPAAQLR
                     DDALSRARFDFRWIDQFNLGLDPDTAKSFHDETLPKEAHKVAHFCSMCGPKFCSMKIT
                     QDVRDYAATLNDPAAVGMAMSGVIEDGMAQMSQKFREMGSNVYLDAEKVKESNRVL"
     gene            complement(2843..3229)
                     /locus_tag="VN968_07590"
     CDS             complement(2843..3229)
                     /locus_tag="VN968_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29138.1"
                     /translation="MSTSIFIARLIGPVMLVIGLAVLTNQRAFREMADEFLASRALLF
                     LSGLIIMPVGLASVLTHNVWTADWLVLITLLGWLTAIGGAVRLCAPEYVMQSGRAMLK
                     RPHFITIAAAFWIVAGLLFCLFGLLH"
     gene            complement(3634..4278)
                     /locus_tag="VN968_07595"
     CDS             complement(3634..4278)
                     /locus_tag="VN968_07595"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXL29139.1"
                     /translation="MPYPDRHAYPDRNAYPDRFYPVVDSVAWVARLTKLGVGTIQLRA
                     KNLDDAQSLQIVSDALEVTKGTPTKLVVNDYWRAAVVAGARHLHLGQEDLAGADLAAI
                     RDAGLTLGLSTHDDAELETALRAKPDYIALGPIFPTTLKSMRFAPQGIAKITEWKKRV
                     GNIPLVAIGGIKLEQAREIFAAGADSIAVVSDVTQNADPDVRVRAWLGLNAEAA"
     gene            complement(4265..5047)
                     /locus_tag="VN968_07600"
     CDS             complement(4265..5047)
                     /locus_tag="VN968_07600"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HXL29140.1"
                     /translation="MLNFYGKSFTSRLLVGSALYPSPAIMQGAIRASGAEIVTVSLRR
                     EAAGGKTGDAFWSLIRELDVTVLPNTAGCRSVREAVTTAKLARELFATSWIKLEVIAD
                     NDTLQPDVVGLVEAADILIKDGFEVFPYCTEDLSVAMRLVETGCKVVMPWAAPIGSAR
                     GITNRDSLKLLRERLPGIALVVDAGLGAPSHAAEALELGYDAVLLNTAIAKAADPVAM
                     AKAFRLGVEAGRAAYEAGLMEARDFASPSTPVVGTPFWHAVS"
     gene            complement(5222..5419)
                     /gene="thiS"
                     /locus_tag="VN968_07605"
     CDS             complement(5222..5419)
                     /gene="thiS"
                     /locus_tag="VN968_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HXL29141.1"
                     /translation="MRVTVNGEQREIASKSVEALLGELEYEGTHFAIALNYDVLPKSR
                     WSQTQLNNGDEIEIITPRQGG"
     gene            complement(5410..6426)
                     /locus_tag="VN968_07610"
     CDS             complement(5410..6426)
                     /locus_tag="VN968_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL29142.1"
                     /translation="MYQGTLSSRGDSPVSVIGAGIAGAWQALLFAQAGHAVTLHERSD
                     QGMTQSTSYWAGGMLAPWCEAETSEPLISRLGIRSLELWREHVPDTPFNGSLVVAHPR
                     DRADFERFARLTTGHKRLDASALDEIEPSLEGRFRDGLFYADEGHVEPRRVLPELHAR
                     FAAAGGTIKFNSDPTNHDLDGVVIDCRGVFARDRQNDLRGVKGEIILIETDEVELSRP
                     VRLIHPRWPLYVIPRGGNRFMLGATSIETEDSGVSVRSALELLGAAYAVHPAFAEARI
                     VEFGSALRPAFPDNLPRIAIDKERIAVNGLYRHGFLLAPALAELTLGYVERGVIDNEV
                     MQCA"
     regulatory      complement(6414..6556)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         1211 a         2221 c         2223 g         1266 t
ORIGIN      
        1 gcgccatcca cgcgaaaacc ggcgcggaca aaccggttcg aaagcgaccg gggcaagcgg
       61 ggatgcgcgc aatgcgattc ccacgacttc atcaccgcag ccatccgctc cggcttttcg
      121 gaatgccaga cgaccgcgtc ccagtccgtt gccacgatta cggcccggcc gcctggcttc
      181 aggacacgga acgcctcggc aagaacgcga tcgacgtcgg gcacgtattc ggcgacctgg
      241 gtgcagacaa cgacgtcgaa tgacgcatcg ggctgcgcga tctgcgttgc atcgccaacc
      301 gcataggaaa gccatttcgg cggatttcgc gttttgcaaa gcgcgatcag gtcgctcgaa
      361 atatcgatgc cggtgacagc cccgtcgctg ccaacggcgg cggcgatgct ttcgcacaga
      421 aagcccgggc cgcagccgat atccagcacc cgctcgcctg gcgaaagcgc gagctgcttc
      481 atggtctccg cccgctgtgc caccacatcc ggggtgaggt agaccctcgc caaatgtcgc
      541 gcggcgtctt cggtgaaggt gagtccgctc atggttgcct cagatggatg gatgcccgcg
      601 tcaagcacgg gcatgatggt aactccgctt catccttgag gagcatcgcc aacgggtcgc
      661 gcgaatgcgc gcccgatgac aggctccgcg atgcgtctcg aaggatgcag gacggccaca
      721 tggttcgaga cgcgcagctg tgctgcgctc ctcaccttga ggctcacctc acaacacccg
      781 attactctcc ttcaccttct ccgcatcgag atacacgttg ctgcccatct ccctgaattt
      841 ttgcgacatc tgcgccatgc cgtcctcgat cacgcccgac atcgccatcc ccacagccgc
      901 cggatcgttc agcgtcgcgg cgtagtcgcg gacgtcctgc gtgatcttca tcgagcagaa
      961 ctttgggccg cacatcgagc agaaatgcgc caccttgtgg gcttccttcg gcagcgtctc
     1021 gtcgtggaag ctttttgccg tgtccggatc gagcccgagg ttgaactggt cgatccagcg
     1081 gaagtcgaac cgcgcgcgcg agagcgcgtc gtcgcgcagc tgcgcggcgg ggtggccctt
     1141 ggcgagatcg ctggcatgcg ccgcgatctt gtaggtgatg acgccggtct tgacgtcgtt
     1201 gcggtcgggt agcccaagat gctccttcgg cgtcacatag cacagcatcg cgcagccgaa
     1261 ccagccgatc atggcggcgc cgatgcccga ggtgatgtgg tcgtagcccg gcgcgatgtc
     1321 ggtggtcagc ggccccagcg tatagaacgg cgcctcgccg cactcctgaa gctgcttgtc
     1381 cacgttgatc ttgatcttgt gcatcgggat gtggccgggg ccttcgatca tcacctggca
     1441 gcctttgtcc cacgcgatct tcgtcaattc gccgagcgtc tccagttcgg cgaattgcgc
     1501 gcggtcgttg gcgtcggcga tcgagccggg acgcaggccg tcgccgagcg agaacgatac
     1561 gtcatatttg cgcatcaggt cgcagatctc gtcgaatctt gtgtagagga aactctcctt
     1621 gtgatgcgcc aggcaccact tcgccatgat cgagccacct cgagagacga tgccggtgac
     1681 gcggttggcg gtcaggtgga tgtagggcag ccgcacgccg gcgtggatgg tgaagtaatc
     1741 gacgccctgt tcgcactgct cgatcagcgt gtccttgtag acctcccagt cgagttttac
     1801 gggatcgccg ttgaccttct ccagcgcctg gtagatcgga acggtcccga tcgggatcgg
     1861 cgcgttgcga agaatccatt cgcgggtggt gtggatgttg cgcccggtcg aaaggtccat
     1921 cacggtgtcg gcgccccagc ggatcgccca caccatcttg tcgacttctt cctccaccga
     1981 cgacgtcacc gcggagttgc cgatattggc gttgatcttg gtgagaaaat tgcggccgat
     2041 gatcatcggc tcgagctcgc catggttgat gttgctggga atgatcgcgc gtccccgcgc
     2101 gatctcggag cgcacgaatt ccggcgtgat gaaggcgggg atgttggcgc caaaactttc
     2161 gccgtcggcg agcgcggctt ccgcgcgttc gtgctgaact ttgcggccga gattttcgcg
     2221 catcgcgacg tagatcatct ccttggtgat gatgccggcg cgggcgaatt cgagctgcgt
     2281 gatcatggga ggcttttgct cccctccccc ttgcggggag gggttggggg tgggggtagc
     2341 cccacgcacc actgcccgtg tggacccccc accctgacct cgagagcgag cttcgctcgc
     2401 ctcggacccc gcaaggtggg agggaacgga ggcagcgtcc aggccgcgca gcggcttgtg
     2461 aaaggcatcg aacgccttcg ccgcgtgatc ggcggagacg ttgccgttat cgaccggctt
     2521 gacggcgcgg ccttcgtacg cttcgacgcc gccgcgttcg agcacccagg cggtgcggtt
     2581 gcgcggtaga cccttgttga cgtcgatggt cacggccgga tcggtgtagg gaccggaggt
     2641 gtcatacacc gaaagattcg gctcgccggc gccttcgctc agcatgatct cgcgcaatgg
     2701 gacgcggaca tccggcgcct catccggcgt gacgaagatc ttgcgcgagg agggcagcgg
     2761 acccgtggtg acggcgggct tgagttcggc ggatgagacg ggtttgttca tggtgttgct
     2821 cccttccgga gatcaggttc tttcagtgca gaagtccaaa caggcaaaac agcaggccag
     2881 ccacgatcca gaacgcggcg gcgatcgtta tgaaatgagg ccgcttcagc atggcgcggc
     2941 cgctctgcat cacatattcc ggcgcgcaca gccgcaccgc gccgccgatc gcggtgagcc
     3001 aacccaacag cgtgatcagc acgagccagt ccgcggtcca gacattgtgc gtcagcacac
     3061 tggcgaggcc gacaggcatg atgatgagcc cgctgagaaa cagcagcgcc cggctcgcca
     3121 aaaactcgtc cgccatctcg cgaaacgccc gctgattggt aagcacggcg agcccgatca
     3181 cgagcatcac cgggccgatc agccgtgcga tgaagatgga tgtcgacatc ggccatccct
     3241 ttcatgccgc ctgcgcgaac gtcgttggcc aggcgcgcac ccgcgcgtcg aaatcgtcat
     3301 ccccgcgtga gcgcaaggcg ggcatacctg gaggtgcgag ctcttgcgag cttcgaagga
     3361 tgacagagcc gggccgtcgc ccttcgaggg ccgctgaaga agcggccacc tcagggtgac
     3421 gggtcgtagg gtatgcgatg acgctgccgg cagctccttc cgtcattgcg aggagccaac
     3481 gggtcccgcc ttcggcgggc ccgatgataa actccgcgac caagcaatcc attcgtgcca
     3541 gcacgcgcgg atacatggat tgcttcgcgg agcctgtcat cgggcggcgc tttgcgccga
     3601 cccgttggct cgcaatgacg ggtgagagct tcatcacgcg gcctccgcgt tcagtccgag
     3661 ccaggcgcgc acccgcacgt cggggtcggc gttctgggtg acgtcgctga cgaccgcaat
     3721 cgaatcggcg ccggcggcga aaatctcgcg cgcctgttcg agcttgatgc cgccgatcgc
     3781 gaccagcggg atgttgccga cgcgtttctt ccattcggtg atttttgcga tgccctgcgg
     3841 cgcgaaccgc atcgatttca gcgtggtcgg aaaaatcgga ccgagcgcga tgtagtcggg
     3901 cttggcgcgc aacgcggttt cgagctcggc gtcgtcatgg gtggagagcc caagcgtcag
     3961 gccggcgtcg cggatcgcgg caagatcggc gccggcaagg tcttcctggc cgagatgcag
     4021 gtgtctggcg cccgcaacca cggccgcgcg ccagtaatca ttgaccacga gttttgtcgg
     4081 cgtgcctttt gtcacttcga gcgcatcgct gacgatctgc agcgactggg cgtcatcgag
     4141 gtttttggca cgcaactgga tcgtgccaac gccaagcttc gtcagccgcg cgacccaggc
     4201 gacgctgtcg acgacaggat aaaaccgatc aggatacgcg ttgcgatcag gatacgcgtg
     4261 gcgatcagga tacggcatgc cagaacggtg tcccaaccac tggagttgaa ggggatgcaa
     4321 aatcgcgggc ttccatcagc ccggcctcat aggccgcgcg tccggcctcg acgcccaggc
     4381 gaaacgcttt tgccatcgcg acggggtcgg cggctttggc gatcgcggtg ttcaatagca
     4441 cggcgtcgta gccgagttcg agcgcttctg ccgcgtgcga gggcgcgccg aggccggcat
     4501 cgaccaccag cgcgatcccg ggcaggcgct cgcgcaaaag tttcaggcta tcgcggttgg
     4561 tgatgcccct ggcgctgccg atcggcgccg cccacggcat caccaccttg caccccgttt
     4621 cgaccagccg catcgcgacg ctcagatctt cggtgcaata ggggaacacc tcaaacccgt
     4681 ccttgatcag gatgtcggcg gcttcgacca ggccgaccac gtccggctgc agcgtgtcgt
     4741 tgtcggcgat cacctcgagc ttgatccagg acgtggcgaa aagttcgcgc gcgagctttg
     4801 ccgtggtcac ggcctcgcgc acgctgcggc agccggcggt gttcggcagc accgtgacat
     4861 cgagttcgcg gatcagcgac cagaacgcgt cgccggtctt gccgccggcg gcctcgcgcc
     4921 gcagggacac cgtgacgatc tcggcgcccg aggcgcggat cgccccttgc atgatcgcgg
     4981 gcgagggata cagcgcgctg ccgaccagga ggcgggaggt gaaggatttg ccgtagaagt
     5041 tcagcatggc gtcagtgtcc tgtcctgccg caagccgcgg cccgtcatcc tgaggtgcgc
     5101 gctcttgcgc gcctcgaagg atgacatcgg tgtgcacgcg gccatccttc gaggctcgcc
     5161 gaaggcggct cgcacctcag gatgacggca gaatttgtgg tggcaagtct cgctcatccc
     5221 atcaccctcc ctgccgcggc gtgatgatct cgatctcgtc gccgttgttc agttgggtct
     5281 gcgaccaccg gctcttcggc agcacgtcgt aattgagcgc gatcgcaaaa tgcgtgccct
     5341 catattcgag ttcgcccaac agcgcctcga cgctctttga agcgatctcg cgctgctcgc
     5401 cgttgacggt cacgcgcatt gcattacctc gttgtcgatc acgccgcgct caacgtagcc
     5461 cagcgtcagc tcggccagtg ccggcgccag caggaaacca tgacgataaa gcccgttcac
     5521 cgcgatcctc tccttgtcaa tcgcgatgcg tggcagattg tcgggaaatg ccggccgcaa
     5581 ggccgagccg aattcgacaa tgcgcgcctc ggcgaaggcc ggatgcacgg cataggcggc
     5641 gcccaataac tccagggcgg agcggacgct gacgccgcta tcctcggtct cgatcgaggt
     5701 cgcgcccagc atgaagcggt tgccgccgcg cgggatcaca taaagcggcc agcgcggatg
     5761 gatcaggcgc accggccgcg acaattctac ctcgtcggtc tcgatcagga tgatctcgcc
     5821 cttgacgccg cgcaggtcgt tttgcctgtc gcgcgcgaaa acgccacggc aatcgatcac
     5881 gacgccgtcg agatcatggt ttgtcggatc gctgttgaat ttgattgtgc cgccggcggc
     5941 ggcaaaacgc gcgtgcaatt ccggcagcac ccggcgcggc tcgacatggc cttcatcggc
     6001 atagaacagg ccatcgcgga agcggccctc gagcgatggc tcgatctcgt ccagcgcgct
     6061 cgcatcaagc cgcttgtggc cagtcgtgag gcgggcaaag cgctcgaagt cggcgcggtc
     6121 gcgcggatgc gccacaacca gcgagccgtt gaacggcgtg tccggaacat gctcgcgcca
     6181 aagctcgagc gaacggatac cgagccggct gatcaggggt tccgaggtct cggcctcgca
     6241 ccagggtgcg agcatgccac cggcccagta gctggtcgac tgtgtcatcc cctggtcgct
     6301 gcgctcatgc agcgtaaccg catggccggc ctgcgcaaac aacagcgcct gccacgctcc
     6361 ggcaatgcct gcgccgatga cggaaacagg ggaatccccc cgtgaagata gtgtaccctg
     6421 gtacatccct gtcccttcgc cggcatgacc cggatcaggt tcaaagggtc accgcggtct
     6481 cttaggccct gcatcggaac agagtctcga gcgtgcggtc tctcagctcc tcgtcggaac
     6541 tcccctcgga acagggctaa tgtaggccga tgcggcgggg tgtcaacgcg gccgtccggg
     6601 aaccgcactg aatgtgtacc cggaattcga ccgtgtcccg cacgcggtgc agcgttcttt
     6661 acgccgctcc gcagaaccgg gacctatggc gacgaagttc tcgatggacc cggatcagca
     6721 gcgcacacgc cgcaagtgcg gcgcgctgcg cagcatccgg ggcacgcttc ctaccgattg
     6781 ggaagctgat gtgcgttggc gtcaaccgcg ctcatcgccg ccgctttcgg cggcgcgccg
     6841 gcgagaaccg tttcaacatg cacgatgcgt tggagccgct ggcgctttgc cgcgtaataa
     6901 taaccgcccg cataataggc c
//
LOCUS       DATNOI010000199        28167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_577887,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000199 DATNOI010000000
VERSION     DATNOI010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 28167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28167
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577887"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VN968_07615"
     CDS             <1..481
                     /locus_tag="VN968_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HXL29143.1"
                     /translation="LPDTVKATGASHVLTVMANVDQVQRPESVLPANHLKVSMDDITE
                     QMDGFVAPSDQHIEQVLNFVRGWDRSAPLVVHCYAGISRSTASAFAAACLVNPHRDEI
                     SIARQIRAASPIASPNRLIVSLADKALGREGRMLRALDEMGPGSMMVEGRPFRIDLE"
     gene            501..1478
                     /locus_tag="VN968_07620"
     CDS             501..1478
                     /locus_tag="VN968_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD regulator"
                     /protein_id="HXL29144.1"
                     /translation="MSEQPLTPIEIGLTAAIVAIEGNEPLILTASGGVGDRLAGLPFG
                     PFDAVAHRTFEIGLRAWVEEQAGLRLGYVEQLYTFGDRGRHTQAGDTDAHVASIGYLA
                     LTRAADNASRAAGATFEPWYRFFPWEDWREARPAMIERDIFPELTRWAAQTEQPEAAR
                     ALSRKDRVRLYFGTEGAHWDEERVLDRYELLYEAGLIEEARRDGRPAALSRGSIPALG
                     VAMRFDHRRILATAMGRLRAKLKYRPVVFELLPPEFTLTELQRTVEAISGRHLHKQNF
                     RRLVEAGALVEPTGVMSTQTGGRPAALFRFRREVLQERPAPGLRVRGRR"
     gene            1572..4262
                     /locus_tag="VN968_07625"
     CDS             1572..4262
                     /locus_tag="VN968_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HXL29145.1"
                     /translation="MFDSPLDFLSLVIAIVAVIFARKALNQTVALRARLELMETNASS
                     AAVERPVPVPPPLPVQEVPIAASASDQIRIETTEAEALPGQPAPPPPIPDIPVAASQP
                     GPGFEERIGTRWVVWVGGLTLALGGFFMVRYSIDAGLLGPGVRTLLGGLFALALLAAG
                     EWTRRKESISAIAALPIANIPAILTAAGTAVAFATVYAAYALYGFLVPATAFILLGLV
                     ALGTLAAALLHGPALAGLGIAAAFVTPVLVSSDKPDFWALYIYLAIVTAAAFGLARIR
                     LWRWLAITTVAFALLWTFPCLQCGPSMVGPHAFHVIAGFVLAALLVVCGLMFGPPADE
                     GRIEPISSGSLAAYLAGSAMIVLASFHTDAAIIVFALLVAGTLFVAWRAEAATGAVGA
                     AAIFVFVVFAEWAVRANPDMLVLPGGPLPGIGPAATDSSVSLHPISAAIFAIGFGAAG
                     FLAQGRFASAIIPVVWSAAAVFTPLTLLIALYARIAHLDRSIPFAILAVVLAAAYGAA
                     TEILTKRDNRPGLAISIALFATGALGALALALTFALEKGWLTIALALMSMGTAWISMQ
                     RPIPFLRSLAAVLAGIVVLRIGYEPRIVGDAVGTTPIFNWLLWGYGIPALSFWTASHF
                     LRRRGDDAPLRTVESAAILFTVLLAFMEIRHAVNGGDVYHDNAGLTEIALQVCVALAM
                     AIGLERLRIRTGSIVHNVSAILLTVFAGLASVFGLLLLENPMIWWSIDVGGVFINQIL
                     LGYALPALLALLLSYAVAGRRRAFYANTIAAGALVLALTYVTLEIRRLYHGPVLTSGA
                     TGDAEQYTYSVAWLAFGVALLGIGIVFNSQRARLASAVVIALTILKAFLIDMSTLTGV
                     YRALSFMCLGLVLVAIGWLYQRILFRRQEAPTAVHAAPGT"
     gene            complement(4355..6028)
                     /locus_tag="VN968_07630"
     CDS             complement(4355..6028)
                     /locus_tag="VN968_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HXL29146.1"
                     /translation="MFNFRAPEGELAKSIATDLLNANVMIADADLTITYMNKAVMALL
                     TEAESDIQKDLPRFGVAKLIGSNIDVFHKNPAHQRQMLANLNATHRATIRVGGRSFDL
                     VATPLKNKDGKRLGTVVEWNDAALRLQNLGYTSIVAAIDKAQAVIEFTLDGTVVTANG
                     NFLNALGYSLPEIQGKHHSIFVEPEMRGSEAYREFWAKLNRGEYQAAEYKRIGKGGKE
                     VWILASYNPILDEKGKPFKVVKYATDISAQKLKTADLAGQIAAISMSQAVIEFNLDGT
                     VLTANDNFLHALGYSLAEIKGHHHRMFVDPAERDGAGYREFWASLNRGEYQAAEYKRI
                     GKGGREIWIQASYNPILDMNGKPFKVVKYATDTTAQVIARMKSESVRGMMESVAAGAE
                     ELNASVREISEAMAKSKETAVSAVSRVEVADQQAQRLAAAAEAMSGIVDLIGNITGQI
                     NLLALNATIESARAGEAGRGFAVVASEVKSLANQAKQATDKIGQEIGNLNGISGDVVE
                     ALTSIRQAIQNVSEYVTSTAAAIEEQSTVTSEMSSSMQRAAAQAASIGR"
     gene            6060..6980
                     /locus_tag="VN968_07635"
     CDS             6060..6980
                     /locus_tag="VN968_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL29147.1"
                     /translation="MSEGVAWSLPRIDFIIQINVNFGAGRTTDGLSVLWLVRAGLARG
                     GRHIDPWTRYAIQELPISVMRRKPGPTTNRALLSGGPDPVEKLADLELEAVALPALDG
                     ALCDFTVSAYIGPMARPRSFDPDEALDLARDVFWQKGFQSTSLDDITAATGLAKPSLY
                     AAFGDKNALFLKVLDRYHERIVANAERVLKEGPSARDAIERWLTGFVPFCSGVKGSRG
                     CLSVNAAADGASDQKQVRDRIQRFNRKLEELLRNRLRADRAQFSDAFDPAGSAHTLMA
                     IYMGLMVLAKDAPDAARVRATLNQAMKLLA"
     gene            7033..8007
                     /locus_tag="VN968_07640"
     CDS             7033..8007
                     /locus_tag="VN968_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HXL29148.1"
                     /translation="MQTIQIEAFGNPAEVLKVVDVPDVGAPAEHEVVIALEASPINMS
                     DLLMISGRYGYRPNLPSVMGTEGVGRVIAVGSGVKHLKQGDRTLIPYPAPAWAERIKT
                     DAGRLRPLPSGDVNQFAMLGINPPTAYLMLTDFVALPSGSWVIQNSANSGVGRALIPI
                     AKSLGLKTVNVVRRDDVVAEIKAIGGDVVLVDGPDLAKRVAAETGKAPIALAVDGVGD
                     TSTTNLVGCLAEKGVHVFYSTISGKPSVVPATHLIFHDISMRGFWLANWFANAKPDQI
                     TGMYDRLVPLVASGAISAPIAGTYSFAEIAEAVAVASKKRGKALFTVG"
     gene            complement(8094..9794)
                     /locus_tag="VN968_07645"
     CDS             complement(8094..9794)
                     /locus_tag="VN968_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HXL29149.1"
                     /translation="MSWLNNFKIVFKVSLIVALMAIVTVGTVIFAAHRMRAVDDANTD
                     IVTRMDKSTTMAARAGRRAENYLSSAFQLAAETTDAGNAKFLAQTADSRKAYESAMAT
                     ILKDMPEKAGIIQPTIAGFQKAFAACEPGIQYAATTNTPDENLKAATRLKAECVPSAD
                     AAIQAHAKMVDDLIAGAAKIADDLTDEANSTIRTLWISAGVGLLIGLASAMWIGIKGL
                     SRPIAELKAVMEAFARNNLSADVPGVERRDELGEMARMVEVFKKNGLEVERLKADQQA
                     GELRTAQQRKADMIKLADDFEGAVGEIIDTVSSASTELEASASTLTSTAERAQELTTM
                     VAAASEEASTNVQSVASATEEMASSVNEISRQVQESARMAGEAVGQARTTNDRVSELS
                     KAAARIGDVVELINTIAGQTNLLALNATIEAARAGEAGRGFAVVASEVKALAEQTAKA
                     TGEIGQQISGIQAATQDSVNAIKEISGTIEKLSEISSTIAAAVEEQGAATQEISRNVQ
                     QAAQGTQQVSSNITDVQRGASETGSASSQVLSAAKSLSGDSNRLKLEVGKFLNTVRAA
                     "
     gene            complement(10155..12158)
                     /locus_tag="VN968_07650"
     CDS             complement(10155..12158)
                     /locus_tag="VN968_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HXL29150.1"
                     /translation="MSFLNLRIRGRLYGGFAALLLFCAGLAGFAVWQLGELRTQIAAL
                     ELQSSNTIRVGEIATDLQAIRRAILRYTFDHDEKSYTEADTRLKKASDLLVEAGKTTK
                     SEERRAAYKEIAKNVEELKAKRLALGDAVNQLVAGRASLFADGDKMAEDVQKFVDAAA
                     KTDYAPSAASLEANVLLVRIANWRMLATRDPKGFATFKTNTEKAQAQIAIFEKADLPP
                     NLAALLANVKTGVAKYVEAFNKSGPNLVAGDDLYYRAVTPLTVDAIGKLDGVKESIGK
                     AFAKTNADTEGLISTTITMQEVVAGAAVLLGLLIAFFIARGIIGPLSGLTSGMKELAG
                     GNFGVVLPGLDRKDEVGDMAQAVETFKVKAEEKARDEAEAKVKQDLVAAKQRRADMIK
                     LADDFEGAVGEIVETVSSASTELEASANTLTSTAERAQELTTMVAAASEEASTNVQSV
                     ASATEEMSSSVNEISRQVQDSARMAGEAVDQARKTNDRVSELSKAAARIGDVVELINT
                     IAGQTNLLALNATIEAARAGEAGRGFAVVASEVKALAEQTAKATGEIGQQISGIQAAT
                     QESVNAIKEISGTIEKLSEISSTIAAAVEEQGAATQEISRNVQQAAQGTQQVSSHITN
                     VQRGASETGSASSQVLSAAKSLSGDSNRLKLEVGKFLNTVRAA"
     gene            complement(12332..14359)
                     /locus_tag="VN968_07655"
     CDS             complement(12332..14359)
                     /locus_tag="VN968_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL29151.1"
                     /translation="MKFNRIGNKLGLASLVSILLSVGMVANQSLTEQSITDANQRADV
                     DQQIADNALRAESEMRSMQLAVRGVRLARTKPDVEKLRGEFSAAQAAQAKWLDAAISL
                     AVLPETRELFTRLRASTQKYAEGADEQAQLHTKLLDLNAKRITLSAEWDGRIEKLYAL
                     LGNSEAANRSAAEANLRDADGALNAVRAEGWQFFSTGEAGQKQKLVRAATMLSESLKR
                     ARGFADDKALREGIESLELLVKEFVEASTQAIDADEAKNAISKLRTIPLANQVADLLR
                     TSVPVAQKAVADARANAADKLLQAGRVNLGFGTVIIVVLLGSMIFSFLAIARPMTRLN
                     AAMGRMAAGALDIVIPGADRGDEIGDVAKTISVIRANAEQAVRDEADAKGDQDRVAAK
                     QRKADMTRLADDFENAVGEIVETVSSASTELEASATTLTSTAERAQELATLVAAASEE
                     ASTNVQSVASATEEMSSSVNEISRQVQESARMAGEAVDQARATNDRVGELSKAAARIG
                     DVVELINTIAGQTNLLALNATIEAARAGEAGRGFAVVASEVKALAEQTAKATGEIGQQ
                     ISGIQAATQDSVNAIKEISGTIEKLSEISSTIAAAVEEQGAATQEISRNVQQAAQGTQ
                     QVSANISDVQRGASETGSASSQVLSAAKSLSGDSNRLKLEVGKFLDSVRAA"
     gene            complement(14692..14925)
                     /locus_tag="VN968_07660"
     CDS             complement(14692..14925)
                     /locus_tag="VN968_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29152.1"
                     /translation="MSLYFFRVSHGRYSGAPDHGSEFASREDAWSEMTKVCGNLVGGV
                     SRSLKQDGELQMELLDEAKKPVFRIRLVAESLG"
     gene            complement(15102..15695)
                     /locus_tag="VN968_07665"
     CDS             complement(15102..15695)
                     /locus_tag="VN968_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29153.1"
                     /translation="MLARLVLGALTASAVMAPALAGQIMSGEEARKFVSGKVFAFTCF
                     DGTRGAGRVLDDGGAMGAVQFSGSGPVRHMRLPGNTLQVRGQSVCASIKGLPFEPCFN
                     LDKKDERNFRGSVSGMGFAYCDFRHQGAAQMLMARAVARPRVLHPPEEQTRSADASRT
                     TGSVRVETPRVEHGAIEPVKSEPAKSENVLELRRSTD"
     gene            complement(15807..16793)
                     /locus_tag="VN968_07670"
     CDS             complement(15807..16793)
                     /locus_tag="VN968_07670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HXL29154.1"
                     /translation="MATFKAIRVDKADKGTTVALTQFDEADLMDGDVTLGVEWSTLNY
                     KDGLAVTGKAPVVRRFPMIAGIDLAGTVERSSHPQWKPGDKVICTGWGMGETHLGAYA
                     EKARVKGDWLVRLPQGMSARDAMAIGTAGFTAMLAVLALEKHGLVPKDGPIVVTGAAG
                     GVGSVATAVLSKLGYRVIASTGRMSEAAYLKNLGAAEVIDRSELSGPAKPLAKERWAG
                     GIDSVGSTTLANILSMTKYAGAIAACGLAAGMDLPSSVAPFILRGVCLLGIDSVMCPI
                     ELRRVAWSRLARDLDPGKLVEITHEIGLGEVVAAGAKILAGQVRGRIVVKIL"
     gene            complement(16837..18087)
                     /locus_tag="VN968_07675"
     CDS             complement(16837..18087)
                     /locus_tag="VN968_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL29155.1"
                     /translation="MAITADDLRTEPGIGSWRTPLVIIVCGCVIALLSFGPRSSLGFF
                     VQPMSREFSWGRDVFGLALAVQNLLWGLGQPIAGAIADRFGVLRVMCVGAVLYAGGLV
                     MMRYAATPLSLDLGAGVLIGFGLSGCSFNLVLSAFSKLLPPERRGIALGAGTAAGSFG
                     QFLFAPFGVAMIGNLGWQAALTVFAGLMLLIIPLSLALATPPATSTSLPVVQQQSFKT
                     ALAEAFGHRSYVLLVLGFFTCGFQLAFITVHLPAYLSDRGVSAQTGGWVIATIGLFNI
                     IGSLGVGWLQNKFPKRYILSVIYFARAMSIVAFISFPITTFSAIVFGASTGLTWLSTV
                     PPTSALVALMFGTRWFATLYGFAFVSHQVGGFLGVLLGGVVFEKFGSYTPIWWLSVLF
                     GVLSALINLPIVEAPVVRPVAQPA"
     gene            18462..19574
                     /gene="nadA"
                     /locus_tag="VN968_07680"
     CDS             18462..19574
                     /gene="nadA"
                     /locus_tag="VN968_07680"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HXL29156.1"
                     /translation="MPIAAIYGPDDFGNPVPGRPVTPARTRVSAAERARELPRPSLEW
                     TPEVERATAHLYERVKNVISPVEWPFMAPYVKAINELKKTRGAVILAHNYQTPEIFHC
                     VADIGGDSLQLAIEATKVKSDIIVQCGVHFMAETSKILNPHKTVLIPDQRAGCSLASS
                     ITGADVRLLRERFPGVPVVAYVNTSADVKAEVDICCTSSNALQVVESLHAPTVIFLPD
                     QYLAKYVASQTNIKIIAWKGACEVHERFTGDELRAYREADPTVQIIAHPECPPDVLAE
                     ADFTGSTAHMINWVRKKHPKRVVMITECSMADNVQAELPDVEIMRPCNLCPHMKRITL
                     PKILDSLIYLREEVTIDPDIAEKARRSVERMINLKN"
     gene            19718..21325
                     /locus_tag="VN968_07685"
     CDS             19718..21325
                     /locus_tag="VN968_07685"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HXL29157.1"
                     /translation="MQDQDRDLTRTADDVVIVGGGLAGLFCALKLAPRPVTVISAAPL
                     GQGASTAWAQGGIAAAVAEGDSAEAHAADTIAVGAGLVDETIALGLAREAGARIHDLL
                     RYGVPFDRDLEGKLAVAQEAAHSARRIVHVRGDMAGKAIIAALIDAVRQTPSIRVIEG
                     FAAEALLTEHGAVAGLQLRQAGDAVAKPVSIASRAVVLATGGIGHLYAVTTNPAEASG
                     LGLAIAARAGAVIADPEFVQFHPTAIMIGRDPAPLATEALRGEGATLINDRGERFMQA
                     RHPLAELAPRDIVARGVFAEIAAGRGVLLDATHALGGHFAEKFPTVHASCMSAGIDPA
                     EQPIPVAPAAHYHMGGIAVDSHGRTSLRGLWAGGEVSSTGAHGANRLASNSLLEAVVY
                     AARIAEDIDGTTIARRARLLDVSITPRNCAMPPLTLTNLRAIMTSHVGVIRDSEHLAE
                     AVRSFAAIERDTGNIALRNMATTALLVAASAWSRRESRGAHYRIDYPVEKPALAHRTM
                     TTLAAARNIAASLSETAPARLAQPVIA"
     gene            21340..22218
                     /gene="nadC"
                     /locus_tag="VN968_07690"
     CDS             21340..22218
                     /gene="nadC"
                     /locus_tag="VN968_07690"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HXL29158.1"
                     /translation="MTTPASLLNPEAFLSPLAIDQAVRRALDEDLGRAGDITSIATIP
                     EATHAGATLIARQAGVIAGLPLAVAVFQKLSPDIAIQAHYRDGGTVAAGVHVLTISGP
                     ARAVLTAERTALNFVGRLSGIATLTADYVRHTIGTRLRICCTRKTTPGLRALEKYAVR
                     CGGGFNHRFGLDDAILIKDNHIAVAGGIRPVLARARANVGHLVKIEIEVDTLSQLREV
                     LDTGLADVVLLDNMDIADLTEAVKLTDGRVVLEASGGVTLESIAKIAATGVDYVSSGA
                     LTHSAPNFDVALDIDA"
     gene            complement(22227..22754)
                     /locus_tag="VN968_07695"
     CDS             complement(22227..22754)
                     /locus_tag="VN968_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29159.1"
                     /translation="MSEGKMTDTGTGDKAGPTLAIKPLTECSAGELIRLTSGAWAIVA
                     NDSSARRIFVISGDDAPLTYVLPKDSTEACLSYGTGFRVASVHASFVGMHTFGHEGFD
                     PVGKLIVARPYAHDGRTSRYFAAPAGQPRFLDLDNFQTVSEPLGHRALFKDWEVSISR
                     PGHPEPVAVVKSPAH"
     gene            complement(22980..24368)
                     /locus_tag="VN968_07700"
     CDS             complement(22980..24368)
                     /locus_tag="VN968_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HXL29160.1"
                     /translation="MSVMRNRSKGVRFAPFGLGLCVFALIPTEIGYQDIASLLARQPG
                     VAERWQKRVSAAASTIQVATYSFGRPIGTALPQPPTYLLARLDNQGIDITGSLTRNPI
                     TKVPPRYQASDFPKVDRTLKGDRLVIVRLEPAAPANATPSNEDPSTSNALVAGAKTAA
                     AMPPAGTTPLDPELAEALKAPPLDDSKMAPDAAPPRGGFSVKTASLFFGSSSIGAAES
                     MERWQPGQEPTIVTPGAGPDPDMKILAPPPPAADGPSRAVDSGESIVAKSEVNSDVQR
                     LTPAERLGLFDEKTRAKSEKCLAEAVYFEARGEAVRGQIAVAQVVMNRAFSGFYPTTV
                     CAVVYQNKHHHLACQFTFACDNVADVVREPDMWVRARKIAKAMLDGQLWLPEVGKSTH
                     YHAYWVRPSWVNEMKKMYKFGVHTFYRPRAWGDGSEAPSWGTPAQTAEISAELAEAAK
                     SSAELSDTTVRR"
     gene            complement(24764..27613)
                     /gene="ppdK"
                     /locus_tag="VN968_07705"
     CDS             complement(24764..27613)
                     /gene="ppdK"
                     /locus_tag="VN968_07705"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HXL29161.1"
                     /translation="MAKAASKLKKTAAKSKSSVTARAKAATPASVRKAFKKSPAKAAP
                     KPVSKPVAPEAAKSGKWVFTFGDGKAEGKAELRDLLGGKGANLAEMANLGLPVPPGFT
                     LPTSVCTYFYAHDKSYPGELKAQVEKALDHVGKLTGKAFGDARNPLLVSVRSGGRASM
                     PGMMDTVLNLGLNDKTVEALAEMSGDRRFAYDSYRRFITMYSDVVLGFEHHHFEDILD
                     TFKDGQGYSLDTDLTGDDWVDLVGRYKDAVARETGHDFPQDPHAQLWGAIGAVFSSWM
                     NARAVTYRRLHDIPESWGTAVNVQAMVFGNMSDTSATGVAFTRNPSTGESRLYGEFLI
                     NAQGEDVVAGIRTPQDITEYARNESGSDKASMETAMPDAFKELTRIYTLLEKHYRDMQ
                     DMEFTVEQGKLWMLQTRGGKRTAKAALRIAVELANEGLISKKEAVSRIDPASLDQLLH
                     PTIDPSATRDVIATGLPASPGAAAGEIVFSSDEAAKLQADGRKVILVRIETSPEDIHG
                     MHAAEGILTTRGGMTSHAAVVARGMGKPCVSGCGSIRVDYGRGTMSIGSRTFKTGDVI
                     TIDGSLGQVLAGRMPMIEPKLSGEFGTLMGWADSFRKLGVRVNADTPEDARTAIKFGG
                     EGIGLCRTEHMFFEETRIRTVREMILAEDEQARRAALSKLLPMQRADFVELFEIMKGL
                     PVTIRLLDPPLHEFLPHTQAEIEEVARAMNTDPRRLADRARDLAEFNPMLGFRGCRLA
                     IAYPEIAEMQARAIFEAAVEAEKRPGKAVGLEVMVPLIATKMEFDLVKARIDATALSV
                     MKATGKKLAYQVGTMIELPRACLMAGDIAGTAEFFSFGTNDLTQTTYGISRDDAAGFL
                     GTYIAKGILEIDPFISVDRDGVGELVKIGVARGRKTRPDLKVGICGEHGGDPASVSFC
                     HEIGLDYVSCSPYRVPIARLAAAQAALGKAIASQA"
     gene            complement(27704..27859)
                     /locus_tag="VN968_07710"
     CDS             complement(27704..27859)
                     /locus_tag="VN968_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HXL29162.1"
                     /translation="MTLTVAHISPIMAVIAGLLILIMPRLLNLVVAIYLILSGLIGLG
                     LLKWLHM"
BASE COUNT         5006 a         8937 c         8757 g         5467 t
ORIGIN      
        1 gcttcccgac accgtcaagg ccaccggcgc cagccacgtc ctcaccgtga tggccaatgt
       61 cgaccaggtg cagcggccgg agtcggtact ccccgccaac catctgaaag tatcgatgga
      121 cgacatcacc gaacagatgg atggctttgt cgcgccgtcg gaccagcata tcgaacaggt
      181 cctgaacttt gtccgcggct gggaccgcag cgcgccgttg gtcgtgcatt gttacgccgg
      241 catcagccgc tcgaccgcga gcgccttcgc cgccgcctgt ttggttaatc cgcatcgcga
      301 cgaaatttcg attgcacggc aaatccgcgc cgcctccccg atcgcatcgc cgaaccggtt
      361 gatcgtcagc ctggcggaca aggcgctggg gcgtgaaggg cgcatgctgc gcgcgctcga
      421 cgaaatgggc ccgggcagca tgatggtcga aggccggcca ttccgcattg atctcgaatg
      481 atgcccgcaa acgacgccag atgagcgaac agcctctgac gccgatcgaa atcggcctca
      541 ccgcggccat cgtcgcgatc gaaggcaacg aacccttgat cctcaccgca tcgggcggcg
      601 ttggcgacag gctcgcgggg cttccgttcg gcccgttcga cgcggtcgcg caccgcactt
      661 tcgagatcgg cctgcgcgcc tgggtggaag aacaggcggg attgcggctc ggttacgtcg
      721 agcagctcta taccttcggc gaccgcggca ggcataccca ggccggcgac accgatgccc
      781 atgtcgcctc gatcggctac ctcgcgctga cgcgcgcggc cgacaatgca tcgcgcgcgg
      841 cgggggcgac tttcgaacct tggtatcggt ttttcccatg ggaagactgg cgcgaagcgc
      901 ggcccgccat gatcgagcgc gacatctttc cggaactcac ccgctgggcg gcgcagaccg
      961 agcagcccga ggcggcacgc gcgctgagcc gcaaggatcg cgtccggctg tatttcggca
     1021 ccgagggcgc gcattgggac gaggagcgcg tgctcgaccg ctacgagttg ctttacgaag
     1081 ccggattgat cgaagaggca cgacgggacg gacgccctgc ggcactgtcg cgcggatcta
     1141 tccctgcgct tggtgttgcg atgcgcttcg accaccggcg aatcctggcg accgcgatgg
     1201 ggcggctgcg cgccaaactg aaataccgtc ccgtggtgtt tgaactattg ccgcccgaat
     1261 tcacacttac tgaactgcaa cgcaccgtgg aagcgatctc ggggcggcac ctgcacaagc
     1321 agaatttccg gcgcctggtg gaagccggcg ccctggtcga accaaccggg gtgatgtcga
     1381 cacagactgg tggacggccc gccgcattgt ttcgcttccg ccgcgaggtg ctgcaggagc
     1441 ggcccgcacc gggcctgcgg gtgcgcgggc gacgctgacg gtttgacggc actggcggcc
     1501 ggacggcgcc ggcgtggtgc aaaaatttga cgcggtacgc ccgaacgccg ggtcgatcgc
     1561 ctggaggctt gatgttcgat tcgccgctcg actttctttc gctcgtgatc gccatcgttg
     1621 cagttatctt tgcgcgcaag gcgctcaatc aaaccgtcgc gttgcgcgcg cggctggagt
     1681 tgatggagac aaatgcgtcg tcggcggcgg tggaacggcc ggtgccggtg ccgccgccgc
     1741 ttcctgttca ggaagtaccg atcgctgcat cggcatcgga ccagatcaga attgaaacaa
     1801 ccgaagcaga ggcgctgcct ggccaaccgg cgccgccgcc tccgatcccg gacattccag
     1861 ttgccgcatc ccagcccggt cccggcttcg aggaacgtat cggcacccgc tgggtggtct
     1921 gggtcggcgg cttgacgctg gcgcttggcg gattcttcat ggtccgctat tcaatcgacg
     1981 ccggcctgct cggccccggc gtgcgcacat tgctgggcgg cctgttcgcg ctagcgctgc
     2041 ttgccgcggg cgaatggacc cgccgcaagg aaagcatctc cgcgatcgca gcgctgccga
     2101 tcgccaatat cccggcgatc ctcaccgccg ccgggacagc cgttgccttc gcgacggtct
     2161 acgccgctta tgcgctgtac ggttttctgg ttcccgccac cgccttcatt ctgctcggac
     2221 tggtggcgct gggcacactc gccgcggcgc tgttgcacgg accggcgctg gcgggcctcg
     2281 gcatcgccgc cgccttcgtc acccctgtgc tggtttcttc cgacaaaccg gacttctggg
     2341 cgctgtacat ttatcttgcc atcgtcaccg cggcggcgtt cggcctggcg cggatcaggc
     2401 tgtggcgctg gcttgcgatc acaacagtag cgttcgcgct gctgtggacc ttcccctgcc
     2461 tgcaatgcgg cccgtcgatg gtcggaccgc atgcgttcca cgtgattgcc ggattcgtcc
     2521 tcgccgcatt gctggtagtg tgcggattaa tgttcggccc gcccgccgac gaaggccgga
     2581 tcgagccgat ttcatccggc tcgctcgcgg cctaccttgc gggctcagca atgatcgtgc
     2641 tcgcgagttt ccataccgac gccgccatca tcgtgttcgc tctgctagtg gccggcacat
     2701 tattcgtggc atggcgcgca gaggccgcca ccggcgcggt cggcgcggcg gccattttcg
     2761 tgttcgtggt gtttgccgaa tgggcggttc gcgccaatcc ggacatgctg gtgctgccgg
     2821 gcgggccgct gcccggcatc ggccctgccg ccaccgacag ttcggtctcg ttgcacccga
     2881 tatcggcggc gattttcgcg atcggattcg gcgccgccgg atttttggcg cagggccgct
     2941 tcgcgagcgc catcataccg gtggtatggt cggctgcggc cgtgttcacg ccgctgacgc
     3001 tgttgatcgc gttgtacgcc cgcatcgctc atctcgatcg ctcgatcccg tttgctatcc
     3061 tggcggtggt gctggcggca gcgtatggcg ccgcgaccga aatcctgacc aagcgggaca
     3121 atcggccggg actagcgatt tcgatcgcgc tgtttgcgac cggcgcgctg ggcgcgctgg
     3181 cgctggcgct gacctttgca ctcgaaaagg gctggctcac gattgcgctg gcgctgatgt
     3241 cgatgggcac ggcgtggata tcgatgcaac ggccgatacc attcctgcgc tcgctggcgg
     3301 ctgtcctcgc cggcatcgtg gtgctgcgca tcggctacga gccgcgcatc gtcggcgatg
     3361 cggtcggtac caccccgatc ttcaactggc tgttgtgggg ctacggcatt ccggcgctgt
     3421 cgttctggac cgcaagccac ttcctgcgcc gccgcggcga cgacgcgccg ctgcgcacgg
     3481 tggaatcggc agcgatcctg ttcacggtgc tgctggcgtt catggaaatt cgtcacgccg
     3541 tcaacggcgg cgacgtctac cacgacaacg ccggcctgac cgagatcgcg ctgcaggtat
     3601 gtgtggcgct cgccatggcg atcggcctgg aacggctgcg gattcgcacc ggcagcattg
     3661 tccacaacgt cagcgcgatc ctcctcaccg tctttgccgg actggcgagc gtattcgggt
     3721 tgttgttgct cgagaatccg atgatctggt ggtcgatcga cgtcggcggc gtgttcatca
     3781 atcagatctt gctcggctat gccctgcccg cgctgcttgc gctgctgttg tcctacgcgg
     3841 tcgccggccg gcgccgtgcc ttctatgcca acaccattgc ggcgggcgcg ctggttctcg
     3901 cgctgaccta tgtcacgctt gaaatccgcc ggctctatca cggcccggtg ctgaccagcg
     3961 gcgccaccgg cgacgccgag caatacacct attcggtcgc gtggctggcg ttcggcgtgg
     4021 ccctgctcgg catcggcatc gtcttcaatt cgcagcgcgc aaggctcgcc tccgcggtcg
     4081 tgatcgcact taccattctg aaagcgttcc tgatcgacat gtcgacactg acgggcgtct
     4141 accgcgcgct gtcgttcatg tgcttaggcc tggtgctggt ggcgatcggc tggctgtatc
     4201 agcgcatcct gttccgccgg caggaagcgc caacagccgt gcacgccgcg cccggcacgt
     4261 agcctcggca cttgcgaccg ctatggcgct gcactgcgta gaagcagcgc ggccgaccgc
     4321 gatgcgtcca tcgcaatcaa aatcgcgcca tcgctcatcg cccgatactg gcggcttgcg
     4381 cggccgcgcg ctgcatgctc gacgacatct cgctggtcac cgtactctgc tcctcgatcg
     4441 ccgccgcggt ggaggtgaca tattcgctga cgttttgaat agcctgcctg atggaggtga
     4501 gcgcttcgac cacatcgccg gaaatgccgt tcagatttcc gatctcctgt ccgatcttgt
     4561 cggtggcctg cttggcctgg ttggcaaggc tcttcacctc ggacgcaaca accgcgaacc
     4621 ctcggccggc ctcgccggcc cgtgccgatt cgatcgtggc gttgagcgcc agcagattga
     4681 tctggccggt gatattcccg atgaggtcga cgatgccgct catggcttcg gccgccgcgg
     4741 cgagacgttg tgcctgttga tcggcgactt cgacccggct gaccgccgaa accgcggttt
     4801 cctttgactt cgccatcgcc tcggagattt cgcggaccga ggcgtttaat tcttcggctc
     4861 ccgccgcgac cgattccatc atgccgcgca cgctctcgct cttcatgcgc gcaatcacct
     4921 gagccgtggt gtcggttgcg tatttcacaa ccttgaacgg cttgccgttc atatcgagga
     4981 tcgggttgta ggatgcctga atccagatct ccctgccacc tttgccgatg cgcttgtatt
     5041 ccgccgcctg gtattcgccc cggttcagac ttgcccagaa ttcgcggtag ccggcgccgt
     5101 cgcgttccgc gggatcgacg aacatacggt ggtgatgacc cttgatctct gccagcgagt
     5161 atccgagcgc gtgcaggaaa ttgtcattgg ccgtcagtac ggtgccgtcc aagttgaatt
     5221 cgatcaccgc ttgcgacatg gaaatcgcgg cgatctggcc ggcgagatcg gcggtcttca
     5281 gcttctgcgc ggagatatcg gtggcatatt tgacgacttt gaacggcttg cccttttcat
     5341 caagaattgg attgtaggat gccaggatcc aaacctcctt gcctcccttg ccgatgcgct
     5401 tgtattccgc cgcctggtat tcgcccctgt tgagctttgc ccagaattca cgataggctt
     5461 cgctgccacg catctccggt tcaacgaata tgctgtgatg cttgccctgt atttccggca
     5521 acgaatagcc gagcgcgttg aggaaattcc cgttggcggt cacgacggtt ccgtccaagg
     5581 tgaattcgat aaccgcttgc gccttgtcga ttgcggcgac gatcgaggta tacccaaggt
     5641 tctgcaagcg cagcgccgca tcgttccatt caaccacggt cccaagacgc ttgccgtcct
     5701 tgttcttcaa gggcgtggcg acaaggtcaa aagaccggcc cccgactctg atcgtcgccc
     5761 ggtgcgtcgc attcagattt gccagcatct ggcgctgatg ggcgggattc ttgtggaaga
     5821 cgtcgatgtt ggagccgatc aatttcgcaa cgccaaatcg cgggaggtcc ttctggatgt
     5881 cggattctgc ttccgtgaga agggccatga ccgccttatt catgtaggta atcgtcaggt
     5941 ccgcatccgc gatcatcacg ttcgcgttca gcagatccgt cgcgatcgac ttggcaagct
     6001 cgccttctgg ggcgcgaaaa ttaaacattg agggaatcct ttggatttgc ggtcggtgct
     6061 tgagcgaagg cgttgcatgg agccttccac gcatagattt tatcatccaa attaacgtta
     6121 atttcggcgc aggtagaact acggatggtc tgtccgttct ttggcttgtc agagccggct
     6181 tggcgagggg tggccgtcat atcgatccat ggacgcgcta cgcaattcaa gaattgccga
     6241 tttcggtcat gcgacggaag cccggcccga cgacgaaccg ggccttgttg tcaggcggcc
     6301 ctgaccctgt tgagaagctt gccgacctcg agcttgaggc ggttgctctc cccgcgcttg
     6361 atggcgctct ttgcgatttc acggtcagcg cgtacatagg tccgatggca agaccgcgca
     6421 gtttcgatcc cgacgaggca ctggacctag cgcgcgacgt gttctggcaa aaaggcttcc
     6481 agagcacatc gctcgacgac atcaccgccg ccaccggcct cgccaagccg agcctctatg
     6541 cggccttcgg cgacaagaat gcgttgttcc tgaaggtcct cgaccgctat cacgaacgca
     6601 ttgtcgctaa cgccgagcgc gtcctcaagg aagggccctc ggcgcgcgac gcgatcgaac
     6661 gctggttgac cggttttgtc cctttctgct cgggcgtcaa gggaagcaga ggttgcctct
     6721 cggtcaatgc cgcagccgac ggcgcctccg accagaagca ggttcgcgac aggatccaac
     6781 gcttcaaccg gaaactggag gaattgctgc gcaaccggct tcgcgctgac cgtgcgcaat
     6841 tttcggatgc gttcgatccg gcgggcagcg cacatacgct gatggcgatc tatatgggac
     6901 tgatggtgct ggcgaaagac gcgccggatg cggcccgtgt ccgcgccacc ctaaatcagg
     6961 cgatgaagct acttgcttga cacctgatct ccattatgta ccttatagta cataacatag
     7021 gagaacatgg tcatgcagac gatccagatt gaagcgttcg ggaatccggc tgaggtcttg
     7081 aaagtggtcg acgttcccga tgtcggcgcg ccggccgaac acgaggtggt gatcgcgctc
     7141 gaagcctcgc cgatcaatat gagcgatctc ttgatgattt ccggccgcta cggctatcgg
     7201 ccgaacttgc caagcgtgat gggaacggaa ggcgttggcc gcgtcatcgc cgtcggcagc
     7261 ggtgtgaagc atctgaaaca gggcgaccgc accctgatac cctatcccgc gcccgcatgg
     7321 gcggaacgca tcaagacgga tgcgggccgg cttcggccgc tgccgagcgg cgacgtcaac
     7381 cagttcgcca tgctgggcat caatccgcca accgcatatc tgatgctgac cgactttgtc
     7441 gccttgccat cgggatcctg ggtcattcag aacagtgcca actccggcgt cgggcgggca
     7501 cttattccga tcgcgaaatc gctcgggctg aagaccgtca atgtcgtgcg gcgagacgat
     7561 gtcgttgccg agatcaaggc catcggcggc gacgtcgtcc ttgtcgatgg accggatctc
     7621 gccaagcggg ttgcagccga gaccggcaaa gccccgattg ccctggccgt ggacggcgtc
     7681 ggcgacacgt cgacgaccaa cctcgttggg tgcctggccg aaaagggcgt gcacgtgttc
     7741 tacagcacga taagcggcaa gccatccgtc gttcccgcga cgcaccttat tttccacgac
     7801 atttcgatgc gcggattctg gctggccaat tggttcgcga acgcgaagcc cgatcagatc
     7861 accgggatgt acgatcgtct tgtgccgctg gtcgcgtcgg gtgcgatcag cgcgccgatt
     7921 gccggcacct acagtttcgc ggagatcgcc gaggcggtag ccgtggcatc aaaaaagcgc
     7981 ggcaaggcgt tgttcacggt aggttgaacc agccggcggc ttggcatccg catgatgccc
     8041 gcttgtggcg aacgtcatgg aggccggctc agcaagagcc ggcctccgct ttgtcaggcg
     8101 gccctcaccg tgttgaggaa cttgccgact tcgagcttga ggcggttgct gtcgccggac
     8161 agtgactttg ctgcggaaag aacctgcgac gaggccgatc cggtctcgct ggcgccgcgc
     8221 tgcacgtcgg taatgttgga ggagacctgc tgcgtgccct gcgcggcctg ctggacgttg
     8281 cgggaaatct cctgggtcgc ggcaccctgc tcttccacgg cggccgcgat ggtcgaggag
     8341 atctcggaca gtttttcgat ggtgccgctg atttccttga tcgcgttcac cgagtcctgc
     8401 gtcgcggcct ggatgccgct gatctgctga ccgatctcgc cggtggcctt cgcggtctgc
     8461 tccgccaggg cttttacttc ggaagcgacg accgcaaaac cgcggccggc ttcgccagcg
     8521 cgcgccgcct cgatggtggc attcaaagcg agcagattgg tctgtcccgc gatggtattg
     8581 atcagctcaa ccacatcgcc gatgcgagct gcggccttcg acaattcgct gacccggtcg
     8641 ttggtggtcc gggcttggcc gacggcttcg ccggccatcc gggccgattc ctgaacctga
     8701 cggctgatct cgttgaccga cgacgccatc tcctcggtcg cggaggccac cgactgcaca
     8761 ttggtggagg cttcctcgga ggccgcggcg accatcgtcg tcagttcctg cgcacgttcc
     8821 gcggtcgaag tgagcgtact tgccgaagcc tcgagctcgg tcgaagccga cgacacggtg
     8881 tcgatgatct cgccgaccgc gccttcgaaa tcgtcggcga gcttgatcat atcggccttg
     8941 cgctgctggg cggtgcgcag ttcgccggcc tgctggtcgg ccttcaggcg ttcgacttcg
     9001 aggccgtttt tcttgaacac ttccaccatg cgggccattt cacccagctc gtcgcggcgt
     9061 tcgacgcctg gcacatcggc gctcaaattg ttgcgggcaa atgcctccat caccgccttc
     9121 aactcggcga tcggccgcga cagacccttg attccgatcc acatcgcgga ggccaggcca
     9181 atcagcagac cgacgcccgc cgagatccag agcgtgcgga tcgtggaatt ggcctcgtcc
     9241 gtcagatcgt ccgcgatctt ggcggcgcct gcgatgagat cgtcgaccat cttcgcatgc
     9301 gcctggatag cagcatcggc ggacgggacg cattcggcct tgagccgcgt ggcggctttc
     9361 aggttttcgt caggcgtgtt cgttgttgcc gcgtattgga tgcctggttc gcacgccgca
     9421 aatgctttct gaaaaccggc gatggtcggt tgaatgatgc ccgccttttc cggcatatcc
     9481 ttcaggattg tcgccatggc ggactcgtag gctttccggc tgtcagcagt ttgcgcgagg
     9541 aacttggcat tgcctgcatc ggtggtttcc gccgccaatt gaaacgcgga ggacaggtaa
     9601 ttttcggccc ggcgaccggc acgggccgcc atggtggtgg atttgtccat gcgggtgacg
     9661 atatcggtgt tggcgtcgtc caccgccctc atccgatgag cagcaaaaat gacggtcccg
     9721 accgtgacaa tggccatcag cgcgacgatc aggctgacct tgaaaacaat cttgaaattg
     9781 ttgagccacg acatgttcgg tcccccaagg aagttggcgt taccagccgc gtggagcgca
     9841 accggtcgtg ttcggcgaag cggaaatccg ccgtcaccga tttatggtgc gtgtttgttc
     9901 gaaactcgga acatccgtgc ccctaagcaa tagcgctcgc ggcacttcgt cagggttaat
     9961 ttcggatacc gtgcaactac gggcatccag ccaaaacacg cggcatcgcg tggtaatggc
    10021 gtggcacagg accgtcttga tcgatgtccc ggcaaccaaa tggctgtcca ggctcgtcaa
    10081 ttatcggatc gccgaagaac gcatgatcgc aagcgggact ccagccctgg tcgggccgga
    10141 cttcctgatg atcgtcaggc cgccctcacc gtgttgagga acttgccgac ttcgagcttg
    10201 aggcgattgc tgtcgccgga cagcgattta gccgccgaca acacctgcga cgaagccgag
    10261 ccggtctcgc tggcaccgcg ctgcacgttg gtgatatgag aactcacctg ctgggtgccc
    10321 tgcgcggcct gctggacgtt gcgggaaatt tcctgggtcg cggcaccctg ctcttccacg
    10381 gcggccgcga tggtcgagga gatctcggac agtttttcga tggtgccgct gatttccttg
    10441 atcgcgttga ccgactcctg ggtcgcggcc tggatgccgc tgatctgctg accgatctcg
    10501 ccggtggcct tcgcggtctg ctcggccagg gcttttactt cggaagccac caccgcaaaa
    10561 ccgcggccgg cttcaccggc gcgcgccgcc tcgatggtgg cattcaaggc gagcagattg
    10621 gtctgtccgg cgatggtgtt gatgagttca acgacgtcgc cgatgcgggc tgccgccttc
    10681 gacaactcgc tgacgcggtc gttggtcttg cgcgcctgat ccacggcttc gccggccatc
    10741 cgagccgaat cctgaacctg acggctgatc tcgttgaccg acgacgacat ctcttcggtc
    10801 gccgaggcca ccgactgcac attggtggag gcttcctcgg aggccgcggc gaccatggtg
    10861 gtgagttcct gcgcgcgttc cgcggtcgag gtgagtgtgt tggctgaggc ttcgagttcc
    10921 gtcgaagccg acgatacggt ctcgacgatc tcgccgaccg cgccttcgaa atcatcggcg
    10981 agcttgatca tatccgccct gcgctgcttt gctgcgacca gatcctgctt cactttggct
    11041 tccgcttcgt cgcgggcctt ctcctcggcc ttgaccttga aggtctcgac cgcttgcgcc
    11101 atgtcgccga cttcgtcctt gcggtcgagg ccgggaagca ccacgccgaa attgccgccg
    11161 gccagttcct tcatgccgga ggtgaggccg gagagcgggc cgatgatgcc gcgagcaatg
    11221 aagaacgcga tcagcaggcc aagcagcaca gctgcgccgg ctacgacttc ctgcattgtg
    11281 atggtggtgc tgatcaggcc ttcggtatct gcgttcgtct tggcgaaagc tttcccgatc
    11341 gattccttca ccccgtcgag ttttccgatt gcgtcgacgg tcaggggcgt gacggctcta
    11401 taataaaggt catcgcccgc caccaggttg ggcccggact tgttgaaagc ctcaacgtat
    11461 ttggcgacac cggtcttgac gttcgccagc agcgccgcga ggttcggcgg caggtcggcc
    11521 ttctcgaaaa tcgcgatctg cgcctgggcc ttttcggtgt tggtcttgaa ggtcgcgaac
    11581 cccttggggt cgcgggtcgc cagcatccgc cagtttgcga tgcgaaccag aagtacgttg
    11641 gcttcgaggg aggctgcgct cggggcataa tcggtttttg ccgcggcatc gacgaatttc
    11701 tggacgtcct ccgccatctt gtcgccgtcg gcgaacagcg atgcgcggcc ggcgaccaac
    11761 tgattgacgg cgtcgccgag cgcgaggcgc ttggccttca attcctcgac gttcttggcg
    11821 atctccttat aggccgcacg ccgttcttcg gacttggtcg tcttgccggc ctccacaaga
    11881 agatcgctcg ccttcttcaa ccgtgtatcg gcttcggtgt aggacttctc gtcgtgatcg
    11941 aacgtgtagc ggaggatcgc gcgacggatc gcctggaggt ctgtggcgat ttcgccgacc
    12001 cggatggtgt tgctagactg cagctccagc gccgcgatct gggtacgaag ttctcccaac
    12061 tgccaaaccg cgaatccggc aaggcccgcg caaaacagca gaagcgccgc gaagccgcca
    12121 tacaagcgac cgcgaatccg aagattgaga aacgacatga ggtcaatcct tgctggcggt
    12181 gagttgaaat cggggagacg aacatcgcca cgtttggcga acttcacaat tttcgttcga
    12241 agagcggatc gagcgctctc gcgtccgaca aaatacggct cgtaacgcta cgtcagggtt
    12301 aattacagcg cccgtaggaa tacgggggac gtcaggcggc cctgaccgag tcgaggaatt
    12361 tgccgacctc gagcttgagg cggttgctgt cgccggacag tgacttcgcc gcggagagaa
    12421 cctgcgacga ggccgatccg gtctcgctgg cgccgcgctg cacgtcgcta atgttggcac
    12481 tgacctgctg ggtgccctgc gcggcctgct gcacgttgcg ggaaatttcc tgggtcgcgg
    12541 caccctgctc ttccacggca gccgcgatgg tcgaggagat ctcggacagt ttttcgatgg
    12601 tgccgctgat ttccttgatc gcgttcaccg agtcctgcgt cgcggcctgg atgccactga
    12661 tctgctggcc gatctcgccg gtggccttcg cggtctgctc cgccagggct tttacttcgg
    12721 aagcgacgac cgcaaaaccg cggccggctt cgccagcgcg tgccgcctcg atggtggcgt
    12781 tcagggccag gaggttggtc tgtccggcga tggtgttgat gagttcaacg acgtcgccga
    12841 tgcgggccgc cgcctttgac aactcaccga cgcggtcgtt ggtggcgcgg gcctgatcga
    12901 ccgcctcgcc ggccatccgg gccgattcct gcacctgacg gctgatctcg ttgacggatg
    12961 aggacatctc ctcggttgcg gaagccaccg actggacatt ggtggaagct tcctcggatg
    13021 ctgctgcgac caaggtggca agctcttgcg cgcgctccgc ggtcgaggtg agcgtcgtgg
    13081 ccgatgcctc cagttcggtc gaagccgacg acacggtttc gacgatctcg ccgaccgcat
    13141 tttcgaaatc gtcggcaagc ctggtcatat cggccttgcg ttgcttggcc gccactcgat
    13201 cctgatcgcc cttggcgtcc gcttcgtccc ggaccgcctg ttcagcgttc gcgcgaatga
    13261 cgctgatggt cttggcgacg tcgccgatct cgtcgccccg gtcagcaccg gggatgacaa
    13321 tgtcgagagc gcctgcggcc atccttccca tcgcggcgtt cagcctggtc atcggtctgg
    13381 cgatcgccaa gaaggagaag atcatcgatc cgagcagcac cacgatgatc accgtgccga
    13441 atcccagatt gacgcggccg gcctgcagca atttatccgc cgcgttcgcc cgcgcgtcgg
    13501 cgaccgcctt ttgtgcgacg ggcacgctcg tccgcagcag gtcggcgacc tgattggcga
    13561 gcggaatcgt gcgcaatttg ctgatggcgt tcttcgcttc atcggcatcg atggcctgcg
    13621 tgctggcttc gacgaactcc ttgaccagca gttcgaggct ctcgatacct tcgcgcagcg
    13681 ctttatcgtc ggcaaatccg cgggcgcgct tcaatgactc ggacagcatc gtggcggcgc
    13741 ggacaagctt ttgtttttga ccggcttccc cggtcgagaa gaactgccag ccttcggcgc
    13801 ggacggcgtt cagcgcgcca tcggcatctc gcaaattcgc ctcagcggcg ctccggttcg
    13861 cagcttcgct gttgccgagc aacgcataca gcttctcgat gcggccgtcc cactcggcgg
    13921 acagcgtgat gcgcttggca ttgagatcga gcagcttggt gtgaagctgc gcctgttcgt
    13981 cggcgccttc ggcgtatttc tgggtcgagg ccctgagcct ggtgaacagc tcccgtgtct
    14041 ctggcaacac agccagcgag atggcggcat cgagccattt cgcctgcgcc gcttgcgccg
    14101 ctgaaaactc gcccctgagc ttctcgacat caggctttgt ccttgcaagg cgcacgccgc
    14161 ggaccgcgag ctgcatgctc cgcatttccg attcggccct aagggcgtta tcagcaatct
    14221 gctgatcgac gtcagcgcgc tgattggcat cggtgatgga ctgctccgtc aaactctggt
    14281 tggccaccat gccgaccgac agcaggatgc taacgaggct ggcaaggccg agcttgttgc
    14341 cgatgcggtt gaacttgatg gtcatcgcag gattgctttt cgatggtggg gagacaaatg
    14401 gcggcggaat cggaataacc ggaaatctgc gttcgaacga cgagcgctct cccggtaatc
    14461 atacggttca ccggttccat gttcgtttaa gcgcggcacc gtagaactac ggggaatggc
    14521 gtgatcttca cgggtcaccg cacgcagcgc gcgccgcgcg gatggcaacg cagccgtcaa
    14581 cgacgggaaa cccgacgcga agtttgacga tgattgaaac gacgatcggc gaaccggtag
    14641 ctgcctgccg aatgacagcc agcggtggcg acccgagcct ggcggagcag cctagcccag
    14701 cgattccgcc acaaggcgga tcctgaacac cggctttttg gcctcgtcca acagctccat
    14761 ctgcaattcg ccgtcttgtt tcaggcttcg ggaaacgccg ccaacaaggt tgccgcaaac
    14821 cttggtcatc tcggaccagg catcttcacg gctcgcgaat tcggatccgt ggtcgggagc
    14881 gcccgaatag cgaccatggc tgacccggaa aaaatacagc gacattattg aacccatgcg
    14941 ttgggccgcc cccaggcctg tcgaagatcg gcaggttgga tcgggattcg ctaccaacac
    15001 atgaatgtat tcacccgtgt gactacggga tgacgatggg tcacgcgccg ccagtttttc
    15061 gattaagcat gttgcgtgcg ccggccagca ccggcgtcgc gtcagtcggt cgagcggcgc
    15121 agctctagca cgttttccga tttcgcaggc tcggatttga ccggctcgat cgcgccgtgc
    15181 tcgactcgcg gtgtctcgac acgcacggat cccgtggtcc gcgacgcatc ggccgagcgg
    15241 gtctgctcct ccggcggatg cagcacacgc ggccgggcca ccgcccgcgc catcagcatc
    15301 tgcgcggcgc cttgatgacg gaaatcgcaa taggcaaagc ccatgcccga gaccgaacca
    15361 cggaaattgc gctcgtcctt cttgtcgaga ttgaagcacg gctcgaatgg aaggcccttg
    15421 atcgaggcgc agacggactg gccgcggacc tgaagcgtat tgccgggaag gcgcatgtgg
    15481 cgcaccggac cggaaccgct gaattgtacg gcgcccatgg cgccaccgtc atcgagcaca
    15541 cggcctgcgc cgcgggtgcc gtcaaagcag gtaaacgcga acaccttgcc ggagacaaac
    15601 ttgcgggctt cctcgccact catgatctgt ccggcgaggg caggcgccat caccgcactg
    15661 gccgtcaacg cacccaacac taaacgcgca agcatgctaa actccaactt accgagcagg
    15721 ggcaaccgct tgatatcttt acccgatgct taccatacca accgtggcaa cattggagca
    15781 gcttggttgg taaagtctga acaaactcag agaattttta ccacgattct accgcgaacc
    15841 tgcccggcga ggattttagc gccggccgcg acgacctcgc cgaggccaat ttcatgagtt
    15901 atttcaacaa gttttcccgg atccagatcc ctcgcgagcc ggctccaggc gaccctgcgc
    15961 aactcaattg ggcacatcac ggaatcgatg cccagaaggc acacgccgcg taaaataaac
    16021 ggcgccaccg aggacggcag gtccataccg gcggcgaggc cgcacgcggc gattgcgccg
    16081 gcgtatttgg tcatcgagag aatgttggcg agcgtggtcg agccgacgct gtcgatcccg
    16141 ccggcccagc gctcttttgc cagcggcttg gccggccccg acagctcgct gcggtcgatc
    16201 acctcggccg caccgagatt tttcagatag gccgcctccg acatgcggcc ggtcgacgcg
    16261 atcacccgat agccgagttt tgagagcacc gcggtcgcga ccgagcccac gccgccggcg
    16321 gcgccggtca cgacgatcgg gccgtccttg ggcacaaggc catgcttttc cagcgcgagc
    16381 accgccagca tcgccgtaaa accggcagtg ccgatcgcca tcgcatcgcg cgccgacatc
    16441 ccttgcggca ggcgcaccag ccagtcgccc ttgacgcgtg ccttctcggc ataagcgccg
    16501 agatgagtct cgcccatgcc ccagccggtg cagatcacct tgtcgccggg cttccattgc
    16561 ggatgcgacg accgctcgac ggtgcccgca aggtcgatgc ccgcgatcat cggaaagcgc
    16621 cgcaccaccg gcgccttgcc ggtgacggca aggccatcct tgtagttcag cgtcgaccat
    16681 tcgaccccca gcgtgacgtc gccgtccatc aggtcggctt cgtcgaattg cgtaagcgcg
    16741 accgtggtgc ccttgtccgc cttgtcgacc ctgatcgcct tgaacgttgc caacgccaac
    16801 tccccgggct tttttctgat ccttatattt accggatcag gccggctgcg caaccggacg
    16861 cacgaccggc gcttcgacga tcggcaggtt gatcagggcc gacagcacgc cgaacagcac
    16921 cgaaagccac cagatcggcg tatacgaccc gaatttctcg aatacgacgc caccaagcag
    16981 cacgccgagg aagccgccga cctggtggct gacgaaggca aatccataca gcgtcgcaaa
    17041 ccagcgcgtg ccgaacatca gggccaccag cgccgacgtc ggcggcaccg tcgagagcca
    17101 ggtgaggccg gtggaggcgc cgaaaacgat cgcggagaat gtcgtgatcg ggaacgagat
    17161 gaaagcgacg atcgacatcg cgcgcgcgaa atagatcacc gacaggatgt aacgttttgg
    17221 aaatttgttt tgcagccagc caacacccag cgagccgatg atgttgaaca gaccgatggt
    17281 ggcaatcacc cagccgccgg tttgcgcgga aacaccgcga tcggacaggt aggccggcag
    17341 atgcacggtg atgaaagcca gctggaagcc gcaggtaaag aagccgagca cgagcaacac
    17401 ataggagcga tgaccgaacg cttccgccag cgccgtcttg aacgactgct gctgcacgac
    17461 cggcaggctg gtcgatgtcg ccggcggcgt cgcgagcgcg agtgaaagcg gaatgatcag
    17521 cagcatcagg cccgcgaaca ccgtcagcgc cgcctgccag ccaagattgc cgatcatcgc
    17581 cacgccgaac ggcgcgaaca gaaattgccc gaacgatccg gcggcagtgc cggcacccag
    17641 cgcaattccc cgccgctccg gcggcagcag cttgctgaac gccgatagga ccaaattgaa
    17701 cgaacagccg gacagtccga agccgatcag gaccccggcg ccgagatcaa gcgacagcgg
    17761 cgtcgcggcg tagcgcatca tgacgagacc gccggcgtac agaacggcgc cgacacacat
    17821 gacgcgcagc acaccgaagc ggtcggcgat cgcgccggca atcggctggc ccagtcccca
    17881 caacagattt tgcacggcaa gcgcgaggcc gaagacgtcg cggccccatg agaactcccg
    17941 gctcatcggc tgcacgaaaa agccgaggct cgatcgcggc ccgaaactca acagcgcgat
    18001 cacgcagccg cagacaatga tcaccagcgg cgtccgccaa ctgccgatgc cgggctcggt
    18061 gcggagatcg tctgctgtaa tcgccatgca atttccctct ggtggttggc gtcgaaaccg
    18121 aaagacggta gacaaggtta cagggagtta atgcattcgc atgtaaatcc caacctgcat
    18181 ttggccgaaa cttctcgccg caatgcagcg atgcaaatca ggcgggcagc tctgacgaat
    18241 taatctgccg ggcgcctagc ggcaccgccg tatcggtgtt atgtatgatt tgctcgctat
    18301 gagaataagt cgattccctc atcgcgaacc gagcctccgc agcgctgcgg ccaggtccgg
    18361 atcaacgcca cacagctttt gaaaccgccg gccccccgag gccggatttc tcaaaagcta
    18421 aatatgctca agttgagtat atttgaatca aggaggccaa catgcccatc gcagcaatct
    18481 atggtcccga cgatttcgga aatcccgttc ccgggcgccc cgtcaccccg gcgaggaccc
    18541 gcgtgagcgc cgcggagcgc gcgcgtgagc tgccaaggcc atcgctggaa tggacccccg
    18601 aggtcgaacg cgccaccgca catctttatg aacgagtaaa gaacgtgatc tcgccggtcg
    18661 agtggccgtt catggcgcct tacgtgaagg ccatcaacga actgaagaag acgcgcggcg
    18721 cggtgatcct ggcgcacaat taccagacgc cggagatctt ccattgcgtc gccgatatcg
    18781 gcggcgactc gctgcagctc gcgatcgaag ccaccaaggt gaagtccgac atcatcgtgc
    18841 agtgcggcgt gcacttcatg gcggagacct cgaagatcct gaatccgcac aagacggttc
    18901 tgatcccgga ccagcgcgcc ggctgctcgc tggcatcgag catcaccggc gcagatgtgc
    18961 ggctgttgcg cgaacgtttt cccggcgtgc cggtcgtggc ttacgtcaac acatctgccg
    19021 atgtgaaggc cgaggtcgat atctgctgca cgtcgtccaa cgcattgcag gtggtggaga
    19081 gcctccacgc gcccacggtg atctttctcc ccgaccagta tctggcgaaa tacgtggcct
    19141 cgcagacgaa cataaaaatc atcgcctgga agggcgcctg cgaagtccac gagcgtttta
    19201 cgggcgacga actgcgcgcc tatcgcgaag ccgacccgac ggtgcagatc atcgcgcatc
    19261 cggaatgccc gcccgacgtg ctggcggagg ccgatttcac cggctcgacc gcgcatatga
    19321 tcaactgggt ccgcaaaaaa caccccaaac gcgtggtgat gatcaccgaa tgctcgatgg
    19381 ccgacaacgt ccaggccgaa ctgccggatg tcgaaatcat gcggccatgc aacctgtgcc
    19441 cgcacatgaa acggattacg ctgccgaaga ttttggacag cctgatttat ctccgagagg
    19501 aagtcacgat cgatcctgat atcgccgaga aggcacggcg ctcggtcgag cggatgatca
    19561 atctgaagaa ttgagtttca cggtcgtccc ggcgaacgcc gggacccata cggcgcggac
    19621 tcacgtcttt tgcagtgtgt ctgatgcctt ctgtaacaat tgacgccagg gattatgggt
    19681 cccggcgttc gccaggacga caataatgga gtcagacatg caagatcagg atcgtgacct
    19741 cacgcgcacc gccgacgatg tcgtcatcgt tggcggcggt ctcgccggcc tgttctgcgc
    19801 actgaaactg gcgccgcgtc cggttaccgt gatttccgcg gcgcctctgg gccaaggcgc
    19861 ctcgacggcg tgggcgcaag gcggcattgc ggcggcggtc gccgaaggcg acagcgcgga
    19921 agcgcatgcg gccgatacca tcgccgtcgg cgccggcctg gtcgatgaaa cgatagcgct
    19981 gggcctcgca cgcgaagccg gcgcgcgcat ccacgatctg ctgcgttacg gcgtgccatt
    20041 cgatcgcgac ctcgaaggca agcttgcggt ggcacaggag gccgcgcatt ccgcccgacg
    20101 catcgtgcac gtccgtggtg acatggccgg caaggccatc atcgcagcgc tgatcgacgc
    20161 cgtgcgccag acgccgtcga tccgggtgat cgaaggtttt gcagcggaag cacttttgac
    20221 cgagcacggc gcggtcgctg gtctgcaatt gcgccaagcc ggcgacgccg tggcaaaacc
    20281 cgtatcgatt gcgtcgcgcg cggtggtgct ggcgaccggg ggcatcgggc atctctatgc
    20341 ggtgacgaca aatccggccg aggccagcgg actggggctg gcgatcgccg cgcgcgccgg
    20401 cgctgtcatc gccgatccgg aattcgtgca gtttcacccc accgccatca tgatcggtcg
    20461 cgatccggcg ccgctggcga ccgaagcgct gcgcggcgaa ggcgcgacgc tgatcaacga
    20521 caggggcgaa cgtttcatgc aggctcgaca tccgctggct gaactggcgc cacgcgatat
    20581 cgtggcgcgc ggcgtgttcg cggaaatcgc cgccggccgt ggcgtgttgc tcgacgccac
    20641 ccacgcgctc ggcgggcatt tcgccgaaaa atttcccacg gttcatgcaa gctgcatgtc
    20701 cgccggcatc gacccggcgg aacagccgat cccggtggcg cctgccgcgc attatcacat
    20761 gggcggcata gcggtggatt cccacggccg cacctcgctc aggggattgt gggccggcgg
    20821 cgaggtatct tccaccggtg cgcacggcgc caaccggctg gcgtcgaact cgctgctcga
    20881 agccgtggtc tacgccgcgc gcatcgccga ggatatcgat ggcaccacga tcgcccggcg
    20941 ggctcgcttg ctggatgtgt ccatcacacc gcgaaattgc gccatgcctc cgcttaccct
    21001 gacaaatctt cgggcgatca tgacctcgca tgtcggtgtc atccgcgata gcgagcacct
    21061 cgcggaagcc gtgcggtcgt tcgcggccat cgagcgcgac accggcaaca tcgcgctgcg
    21121 caatatggcc acgacagcgc tattggtcgc ggcgtcggca tggtcgcggc gcgaaagccg
    21181 cggcgcgcat taccgcatcg attatcccgt cgagaaaccg gcgctggcgc atcgcaccat
    21241 gacgacgctc gccgccgcgc gaaatatcgc cgcgtcactc tccgagaccg cgccggcgcg
    21301 gttagcacag cccgtcatcg cctgaatgga atgcacatta tgaccacgcc cgcctcgctg
    21361 ctcaatcccg aagcctttct gtccccgctc gccatcgacc aggccgtgcg gcgcgcgctg
    21421 gacgaagatc tcggccgcgc cggcgacatc acctcgatcg ccaccatccc ggaagcaacc
    21481 cacgccggtg cgactttgat tgcccggcag gccggcgtga tcgccggatt gccgctggcg
    21541 gtcgcggtgt ttcagaagct gtcgcccgac atcgccatcc aggcgcatta tcgcgatggc
    21601 ggcaccgtcg cggccggcgt gcatgtgctg accatctccg gaccggcgcg cgctgtgctt
    21661 acggctgagc gcaccgcctt gaattttgtc ggccggctgt cgggcatcgc cacgctgacc
    21721 gccgattatg tgcgccatac catcggcacc aggttgcgga tctgctgcac ccgcaagacc
    21781 acgccgggcc tgcgcgcgct tgaaaaatat gccgtgcgct gcggcggcgg cttcaatcat
    21841 cgcttcggcc tcgatgacgc gatcctcatc aaggacaatc acatcgcggt cgccggcggc
    21901 atcaggccag tgctggcgcg cgcgcgggcg aacgtcgggc atctggtcaa gatcgagatc
    21961 gaggtcgata cgctctcgca gttgcgcgag gtgctcgaca ccggcctcgc cgacgtggtg
    22021 ctgctggaca atatggatat tgccgatctg accgaggccg tgaaactcac ggacggacgc
    22081 gtggtgctgg aggcttccgg cggcgtcacg ctggagtcga ttgcgaagat cgcggcgacc
    22141 ggcgtcgatt acgtctcgtc cggcgcgctg acgcattcgg cgccgaattt cgatgtcgcg
    22201 ctcgacatcg acgcgtaagc gagcgcttag tgagccggag acttgaccac cgcgaccggc
    22261 tcggggtgtc ccggcctgct gatcgacact tcccaatcct taaacaatgc gcgatgtcca
    22321 agcggttccg agacggtttg aaaattatca aggtcaagaa accggggttg gccagctggc
    22381 gccgcgaaat agcggctggt gcgcccgtcg tgcgcgtagg gccgggcaac gatcagcttt
    22441 ccgacgggat cgaacccctc atggccaaag gtatgcatcc cgacgaagct tgcgtgtact
    22501 gacgccactc gaaatccggt gccatagctt agacacgcct ccgtgctgtc cttcgggagg
    22561 acataggtca gcggcgcatc atcaccggaa atcacgaaaa ttcttctggc gctcgaatcg
    22621 ttcgctacaa tcgcccaggc gccgctggtc aggcgaataa gctcgcctgc gctgcactct
    22681 gtcagcggct tgatggcaag agtcggccca gccttgtcgc ccgtcccagt gtcagtcatt
    22741 ttgccctcac tcatatactg atgaggtttg tcgcagcctg cctgttattt cgggcattcg
    22801 tcatctgcgc aattgtgccc gcctgcaacg ataatagccg tcgagacacc atttgattcg
    22861 ggcacaattg tgggagttga agctttgcct gggatatcgc ggcagcggcg gtgtaaccgc
    22921 tcacccgggt cgcgctgagc actcggcgca agcgtaccca ggtttcaaga atcgggtctt
    22981 tagcgccgca ccgtcgtatc cgacagctcc gccgaactct ttgcggcttc cgcgagctcg
    23041 gcggaaatct ccgccgtctg cgccggcgtt ccccagctcg gcgcctcgct gccgtcgccc
    23101 caggcgcgcg gccggtagaa ggtgtggacg ccgaatttgt acatcttctt catttcattg
    23161 acccaggagg gccgcaccca ataggcgtgg taatgcgtcg acttgccgac ttccggcaac
    23221 catagctggc cgtcgagcat cgctttcgcg atcttcctgg cgcggaccca catgtcgggc
    23281 tcgcgcacca cgtcggcgac gttgtcgcag gcgaaggtga actggcacgc aaggtggtgg
    23341 tgcttgttct ggtagaccac ggcgcacacc gtggtcgggt agaagccgga gaacgcgcgg
    23401 ttcatcacca cctgcgccac cgcgatctgg ccgcgcacgg cttcgccgcg ggcttcgaaa
    23461 tagacggctt ccgcgaggca tttctcggat ttggcccgcg tcttttcatc gaacaggccg
    23521 aggcgttcgg ccggcgtcag acgctggacg tcggagttga cttcgctctt ggcgacaatg
    23581 ctctcaccgc tatcaaccgc cctcgacggc ccatccgcgg ccggcggcgg cggcgccagg
    23641 attttcatgt ccggatcggg accggcgccc ggtgttacga tggtcggctc ttgcccaggc
    23701 tgccagcgct ccatgctctc ggcggcgccg atcgacgagc tgccgaaaaa caggctcgcg
    23761 gttttcaccg agaagccacc acgcggcggt gcggcatcgg gcgccatttt cgaatcgtcg
    23821 agcggcggcg ctttcagcgc ttcggcaagt tccggatcga gcggggtagt tccggccggc
    23881 ggcatcgcgg cggcggtctt tgcgccggcc accagggcat tggacgtcga cggatcctcg
    23941 ttcgacggcg tcgcgttcgc gggcgccgcg ggttcgagcc ggacgatgac aaggcgatcg
    24001 cccttcaggg tgcgatcgac cttgggaaaa tcggaggcct gatagcgcgg cggcaccttc
    24061 gtgatcggat tgcgcgtcag cgaacccgtg atgtcgatgc cctgattgtc gaggcgcgcc
    24121 agcagatagg tcggaggctg cggaagagcc gtcccgatcg gacggccgaa gctgtaggtg
    24181 gcgacctgga tggtgctggc ggcggcggac acccgcttct gccagcgctc ggcgacgccc
    24241 ggctgccggg ccagcagcga tgcgatgtcc tgatatccaa tttcggtcgg gatcaatgcg
    24301 aagacgcaaa gaccaagacc gaagggcgcg aacctcacgc ccttcgaccg gttacgcatg
    24361 actgacatcg atacgctcac gcaacactac tcaatacaga cgcacgatcg aaccccagta
    24421 catccgtgca attcgatctt ttttggtgat atcgaattta ggttgcgagg gagttaatcg
    24481 gcgttgcgca cgcgacacac gcaagaactc gctggacttt gcacaatcgc atcaaaaacg
    24541 ttggtaaaca ggcacatgaa tgaggtggta aacatggcgt gaacgaaaat gatgaagaaa
    24601 attttcggca ccctgcaaat ttttccgccg cgtcatcgcg gcacacccgt gatcgcaggg
    24661 atctcggcag gcatgccgcg gcccgcttct tgacgcgcct tgcgaacaac aaatggccgg
    24721 gaagattccc ggccatttgc gtctcaaaac cgatagctga tcgttacgcc tggctggcga
    24781 tcgccttgcc gagcgccgcc tgtgccgcgg ccaaccgcgc gatcgggacg cggtagggcg
    24841 agcacgatac gtaatccagt ccgatctcgt ggcagaacga gaccgaagcc ggatcgccgc
    24901 catgttcgcc gcagatgccg actttcagat cggggcgggt cttgcgcccg cgcgcgacgc
    24961 cgatcttcac caactcgccg acgccgtcgc gatcaaccga aatgaaagga tcgatttcca
    25021 ggattccctt ggcgatgtaa gtgcccagga accccgcggc gtcatcgcgg ctgatgccat
    25081 aagtcgtctg cgtcagatcg ttggtgccga acgagaagaa ctccgccgtc ccggcaatgt
    25141 cgccggccat caggcaggcg cgcggcagtt cgatcatggt gccgacctga taggcgagct
    25201 ttttcccggt cgccttcatc accgacagcg cggtcgcatc gatccgcgcc ttgaccagat
    25261 cgaactccat cttggtcgcg atcaacggca ccatgacttc gaggccgacc gccttgccgg
    25321 ggcgcttctc cgcttcgacc gcggcctcga agatggcgcg cgcctgcatc tcggcgatct
    25381 ccggataggc gatggcgaga cggcagccgc ggaagccgag catcggattg aattcggcga
    25441 gatcgcgggc gcgatccgcc agccggcgcg gatcggtatt catcgcgcgc gcgacttcct
    25501 cgatttcggc ctgggtgtgc ggcaggaatt catgcaatgg cggatcgagc agccggatcg
    25561 tcaccggcag gcccttcatg atctcgaaca gttcgacgaa atccgcgcgc tgcatcggca
    25621 acagttttga gagcgcggcg cggcgcgcct gttcgtcctc ggcgagaatc atctcgcgca
    25681 cggtgcgaat cctagtctcc tcgaagaaca tgtgctcggt gcggcaaagc ccgatgccct
    25741 cgccgccgaa ttttatggcg gtgcgcgcat cctccggcgt atcggcgttg acgcgaacgc
    25801 cgagcttgcg gaaggaatcg gcccacccca tcagggtgcc gaattcgccg gacaatttcg
    25861 gctcgatcat cggcatccgc ccggccagca cctggcccag cgatccatcg atggtgatga
    25921 catcgccggt cttgaaggtg cgcgagccga tgctcatggt gccgcggccg taatcgacgc
    25981 ggatgctgcc gcagccggag acgcagggct tgcccatgcc gcgcgcgacc accgcggcgt
    26041 gcgaggtcat gccgccgcgc gtggtcagaa tgccttcggc ggcgtgcatg ccgtgaatgt
    26101 cttccgggct ggtttcaatc cgcaccaaaa taaccttccg tccgtcggcc tgcagtttcg
    26161 ccgcctcatc ggacgagaac acgatttcgc cggcggcagc ccccggggat gccggcaggc
    26221 cggtggcgat cacgtcgcgg gtcgccgagg gatcgatggt cggatgcagc aattggtcga
    26281 gcgatgccgg gtcgatccgc gacaccgctt ccttcttgga gatcaggcct tcattggcga
    26341 gttcgacggc gatgcgaagt gcagctttcg ccgtgcgctt gccgccgcgg gtctgcagca
    26401 tccacagctt gccctgctcg accgtgaact ccatgtcctg catgtcgcgg tagtgctttt
    26461 ccaaaagcgt gtagattcgc gtcagttcct tgaacgcgtc gggcatcgcg gtttccatcg
    26521 atgccttgtc ggagccggac tcgttgcgcg catattcagt gatgtcctgc ggcgtgcgga
    26581 tgcccgccac cacgtcttcg ccctgggcgt tgatcaggaa ttcgccgtac agcctgctct
    26641 cgccggtgga gggattgcgc gtgaacgcaa cgccggtcgc cgaggtgtcg ctcatattgc
    26701 cgaacaccat cgcctgcacg ttgaccgcgg tgccccagga ctccggaatg tcatgcagcc
    26761 gccggtaggt caccgcgcgc gcgttcatcc acgacgaaaa caccgcgccg atcgcgcccc
    26821 acaattgcgc gtgcggatcc tgcgggaagt catggccggt ttcgcgcgcc accgcgtcct
    26881 tgtagcggcc gaccagatcg acccagtcgt cgccggtcag atcggtatcg agcgaatagc
    26941 cctggccgtc cttgaaggta tcgagaatgt cctcgaagtg atgatgctcg aagccgagca
    27001 ccacgtccga atacatcgtg atgaagcggc ggtagctgtc ataggcgaag cggcgatcgc
    27061 cggacatttc ggccagcgcc tcgaccgtct tgtcgttgag gccgagattg agcacggtgt
    27121 ccatcatgcc cggcatcgaa gcgcggccgc cggaacgcac cgaaaccagc agcggattcc
    27181 tggcatcgcc gaacgccttg ccggtcagct tgccgacatg gtccagcgcc ttttcgacct
    27241 gcgccttcaa ttccccagga taggatttgt cgtgcgcata gaaataggtg cagaccgatg
    27301 tcggaagcgt gaagcccggc ggcaccggca atccgagatt ggccatctcg gcgagattag
    27361 cgcccttgcc gcccagcaaa tcgcgaagtt ctgctttgcc ctcggccttg ccgtcgccga
    27421 aggtgaacac ccatttaccg gatttcgccg cctcgggcgc aacgggtttg ctgaccggct
    27481 tcggcgccgc tttggccgga ctttttttga acgccttgcg gacgctggcc ggcgtcgcgg
    27541 ccttggcccg ggccgtcacg gatgactttg atttcgctgc ggttttcttg agtttcgatg
    27601 cggctttggc catgacttac aatcaatccg cgagagggaa aggggatgcg cgcttacacc
    27661 acgttttgcc ggttccgcgc aaggcgcgaa gccggtttca acgttacata tggagccatt
    27721 tgagcagccc gaggccgatc aggccactga gaatgagata gatcgcgacc accagattaa
    27781 gcagccgcgg catgatcaga atcagcaggc ccgcaatcac ggccatgatt ggtgaaatgt
    27841 gggcgactgt cagggtcatg taaaaattct ccgctggtgt ttgtgaagcc gaatcgagcc
    27901 ggactttatc cggtagagca gttccacgat agcagacgct tgtcgcttca acgggttcca
    27961 gcgcctgcat aaaatgccga aaattgcagc ggaaagatgc gcatgttccc gggaacgatg
    28021 ccgcgcgcga tgaattggga gggattgcgt gccggtgcaa accggcgtgc gtcgtcgcaa
    28081 aggagttcac catgcgaaac agaatactgg ccattgcagc aatcgccggc gcgatcagcg
    28141 taccgatcgc ggcgcaggcg cagagcg
//
LOCUS       DATNOI010000200         2936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_66311,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000200 DATNOI010000000
VERSION     DATNOI010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66311"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VN968_07715"
     CDS             <1..187
                     /locus_tag="VN968_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29163.1"
                     /translation="DSMRLIVGIIIGCALTVGGAYIADTVSAAGAKPVMVNWEVVAKN
                     LDSVTTLARAGWKRIAG"
     gene            complement(283..1233)
                     /locus_tag="VN968_07720"
     CDS             complement(283..1233)
                     /locus_tag="VN968_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020479881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL29164.1"
                     /translation="MRREGSALHGFGVVTLKEIADHFTSILVVVLVVLVVATAIAVVR
                     GAVDQIKEITAEDPFLFLRLFTRGAPIPLVALLSFLVPLFAIGLGFDSVNGEHNRRTL
                     SRILAQPIYRDALLFGKFAAGLFTLSVSLIVLWLLVIGLGLLGLGVPPNAEEMARALV
                     LLVVTMVYAGFWFALALLCSVVFRSSATAALVALGLWLFLTILWPLFSPALADAVTTA
                     GDVLGVLDTRQAFARLSPTVLFNEIVAVVLDPAVRSTQQSMLAALGLALIERGAIPDA
                     PIPLLQSVLIVWPQIVGLVAGAILLFVAAYVVFQRQEVRA"
     gene            complement(1223..2170)
                     /locus_tag="VN968_07725"
     CDS             complement(1223..2170)
                     /locus_tag="VN968_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL29165.1"
                     /translation="MNATVIEAKNLTKRYDGVAVVNGISFSVARGEIFGLLGPNGAGK
                     TTTILMLLGLSDISDGQARVLGHDPAREPLAVKRQVGYLPDQIGFYDGLSAADNLRYT
                     ARLMGLERAEREQRISASLGHVGLADVADNRVSTFSRGMRQRLGVAEILMKEAQIAIL
                     DEPTSGLDPQATVELLEIIRDLKKRGVSVLLSSHLLERVQSVCDRVALFNQGKIALIG
                     TVSELGREVLGGGFRVEVEAEGQGLAERIAAIPGVQQVEPAGANRLRLLATGDVRPEA
                     AAAVVAAGGRLLRLSVEEPSLEAIYSRYFQTHEGELHAT"
     gene            complement(2167..>2936)
                     /locus_tag="VN968_07730"
     CDS             complement(2167..>2936)
                     /locus_tag="VN968_07730"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HXL29166.1"
                     /translation="VKLATDLPAKLTLNPQLPELRGTSKSSFEFQLTIKNDSPKKVVA
                     SLSAQAPQNFDATYTEQYGSQELNALPLDPGQSKDVKLKVRPPNTVAAGKYKVTAKVS
                     ADDATAASELVLDITGQPKIDIAGREGVLSAKASAGQETSIPVILTNSGTAPAEQIEL
                     SGSVPTGWKVNFEPKTVDRIAPNENKEVQALVTPAAKAVAGDYVTTVRAAARGESASQ
                     SFRVAVTTSTIWGIVGIALIGGALLVMIGAVVWFGRR"
BASE COUNT          526 a          977 c          976 g          457 t
ORIGIN      
        1 ggactccatg cgcttgatcg tcggcatcat catcggctgc gcgctgaccg ttggcggcgc
       61 ctatatcgcc gacaccgtct cggccgccgg ggccaagcca gtcatggtga actgggaggt
      121 ggtggcgaaa aacctggaca gcgtcaccac cctcgcgcgc gcgggctgga agcgaatagc
      181 cgggtgattg atcggcgcgc gcactccctt tgcgccctct ggcgactttc ctagagcggc
      241 gcgcagccgc cctcacctgc gattgccgat cgccgagaac gatcaggccc gcacttcctg
      301 gcgctggaag acgacatatg cggcgacgaa gagcaggatc gcgcctgcca ccaggcccac
      361 gatctgcggc cacacgatca gcacgctttg caggagcggt atcggcgcat ccgggatggc
      421 gccgcgctcg atcagggcga ggcccagtgc cgcgagcatg gactgctgcg tcgagcgcac
      481 ggccggatcg agcacgaccg caacgatctc gttaaacaac acggtcggag aaagccgcgc
      541 gaacgcctgc cgcgtgtcga gcacgcccaa gacatcccct gcggtggtga ccgcgtcggc
      601 cagcgccggc gagaacagcg gccagagaat ggtcagaaac aaccacaagc cgagcgcgac
      661 cagcgcagcc gtcgccgacg agcggaacac gaccgagcac agcagtgcga gcgcgaacca
      721 gaagccggca tagaccatgg taaccacgag caagaccagc gcgcgcgcca tctcttcggc
      781 gttgggggga actccaagcc cgagcaaccc gaggccgatc accagcagcc acaacacgat
      841 cagactgacc gacagggtga acagcccggc ggcgaatttt ccgaacagca gggcgtcgcg
      901 atagatcggc tgcgcgagga tgcgcgagag ggtgcggcgg ttgtgctcgc cattgaccga
      961 gtcgaagcca agcccgatcg cgaacagcgg caccaggaag gacagcagcg ccaccagcgg
     1021 tatgggtgca ccgcgggtga acaagcgcag gaacaggaac ggatcttcgg ccgtgatctc
     1081 cttgatttga tcgacggcgc cgcggaccac ggcgatcgcg gtggccacca ccagaacgac
     1141 gagcaccacc acgaggatgc tggtgaagtg atctgcgatc tccttcagcg tcacgacccc
     1201 gaaaccgtgc aaggccgaac cttcacgtcg catgcagctc tccctcgtgg gtctggaagt
     1261 agcgggagta aatcgcctcg aggctcggct cctcgaccga cagacgcaac aggcgcccgc
     1321 cggctgccac caccgcggcc gcggcttccg gccgcacgtc gccggtggcc agcaatcgga
     1381 ggcgattggc gcccgccggt tcgacctgtt gcaccccggg gatcgccgcg atgcgttcgg
     1441 caaggccctg cccttcggct tccacctcga cgcggaagcc gccgccgagc acctcgcggc
     1501 cgagctccga caccgtgccg atgagcgcga tcttgccttg attgaacagc gccacgcgat
     1561 cgcaaacgct ttgcacccgt tcgagcaggt gcgaggacaa cagcacgctc acaccacgct
     1621 ttttgaggtc gcggatgatc tcgagcaact cgacggtggc ctgcgggtcg agacccgacg
     1681 tcggttcgtc gaggatcgcg atctgcgcct ccttcatcag gatttcggcg acgccgagtc
     1741 gctggcgcat gccgcgtgaa aaggtgctga cgcggttgtc ggcgacgtcg gcgagcccga
     1801 cgtggccgag cgaagcgctg atccgctgct cgcgctcggc gcgctccaac cccatcagcc
     1861 gcgcggtgta gcgcagattg tcggcggcgc tgagcccatc gtagaagccg atctggtcgg
     1921 gcaggtaacc gacctgacgc ttgaccgcca gcggctcgcg ggcgggatcg tggccgagca
     1981 cgcgcgcctg cccgtccgag atgtcgctca ggccgagcag catcaggatg gtggtggtct
     2041 tgccggcgcc gttcggaccg agcagtccga aaatctcccc gcgcgccacg gaaaacgaaa
     2101 tgccgttgac gacggccact ccgtcgtagc gcttggtgag gtttttcgcc tcgatgacgg
     2161 tggcgttcat cgccgtccga accacaccac ggcgccgatc atgaccagca gcgccccgcc
     2221 gatgagcgcg atccccacga tgccccaaat ggtggaggtc gtgaccgcga cgcggaacga
     2281 ctgggacgcg gattcaccgc gcgcggcggc ccgcaccgtg gtgacgtaat cgccggcgac
     2341 ggccttggcg gcgggcgtga ccagcgcctg cacctccttg ttctcgttgg gggcaatgcg
     2401 gtcgacggtc ttcggctcga aattgacttt ccagcccgtg ggaaccgagc ccgacagctc
     2461 gatctgctcc gccggcgcgg tgccggaatt ggtgaggatc accgggatcg acgtctcctg
     2521 gccggcggaa gccttggcgc tcagcacccc ctcgcggccg gcaatgtcga tcttcggctg
     2581 gccggtgatg tcgagcacga gttcggacgc ggcggtggcg tcgtcggccg atactttcgc
     2641 cgtgaccttg tatttgccgg ccgcgaccgt gttcgggggc cgcaccttca gcttcacgtc
     2701 cttcgactgg ccgggatcga gcggaagcgc gttgagctcc tggctgccgt attgctcggt
     2761 ataggtggcg tcaaagttct gcggcgcctg cgccgacaag ctcgccacca ctttcttggg
     2821 gctgtcgttc ttgatggtga gctggaactc gaagctcgac ttggacgtgc cgcgcagctc
     2881 gggaagctgc ggattgagcg tgagcttggc aggcagatcg gtcgcgagct tcacct
//
LOCUS       DATNOI010000201         7640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2503799,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000201 DATNOI010000000
VERSION     DATNOI010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7640
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2503799"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VN968_07735"
     CDS             <1..490
                     /locus_tag="VN968_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL29167.1"
                     /translation="YLFADNADLARWQSWLRSTAGSDLAEYKDFGGGVYRAASFAENR
                     IATCLFIGPARDAGDWDVVKNLFAADALSDDQRRMLLSGKSTDGLASAGPIVCACFGV
                     GRTAILAAIAGGARTAAEIGVQLKAGTNCGSCIPELKRLIAQVGAAAPVHEQHMLAAT
                     AS"
     gene            complement(547..1374)
                     /locus_tag="VN968_07740"
     CDS             complement(547..1374)
                     /locus_tag="VN968_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL29168.1"
                     /translation="MSPLRQLLGRIGLGFALIVIVSPAVLFFLWMLSLSVKFEVDNAS
                     YPPVFIPEHFNWGNYAAVIDSKRFSTYFTNSLIVTGAATAFALLIGVPAGYGLARMRA
                     HKAAVVILIARITPGLSYLIPLFLLFQWLGLLGTLWPQIIIHLVVTVPIVIWIMIGYF
                     ETTPMELEESALIDGATRWQVFRYVALPIARPGIAVAFILAVIFSWNNFVFGIVLAGR
                     ETRTLPVAVYNMISFDQLSWGPLAAAALIVTLPVLLLTVFAQRQIVTGLTAGAVKGG"
     gene            complement(1371..2315)
                     /locus_tag="VN968_07745"
     CDS             complement(1371..2315)
                     /locus_tag="VN968_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL29169.1"
                     /translation="MSGLAASEAAPARSGAASRETGGWRPPSYWPFVLPALIVVLAVI
                     IFPWAYTIWMSLHEWKVGAPPTFVGLANYIRLPGDARFVESVWHTLVYTALSVALPLL
                     FGTLAAVVFHAKFPMRGFLRGLFILPMMATPVAIALVWTMMFHPQLGILNYLLSLVGL
                     PPQLWVFHPATVIPSLVLVETWQWTPLVMLIVLGGLAAIPAEPYESAQIDGATRWQVF
                     RYISLPLIMPFLFIASMIRMIDAVKSFDIIFAITQGGPGSASETINLYLYSVAFVYYD
                     VGYASAIVVIFFALIVALAGALLHLRQSTHWNDTGGIA"
     gene            complement(2312..3637)
                     /locus_tag="VN968_07750"
     CDS             complement(2312..3637)
                     /locus_tag="VN968_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HXL29170.1"
                     /translation="MAHFGPTRRSLLQGTLAASALSLTGFPARAEPQWKKYAGTTIEA
                     NLIKGPRGELLQKYAFEFTDLTGIKVESEVIPEQQQRQKAVIELTSGKPSFDVIHVSY
                     HVQKRQFEKAGWLADLTPFMKDPNLTAPDLTVDDFSAGGLQFAQNDKGEMHSLPWSVD
                     YFILYWNKELFAKKGVALPKTFDEMATAAEKLNDPANGIYGFTGRGLRNANMTLWTNF
                     FLNYGGEFLDAKGNILTDGPEAIEATKMYQRLLTKTAPPGVAGFNWMESMASLTQGRA
                     AMWIDGVGWAPPIEDPNASRVVGKIGYALVPAGPKGQYSAAYGDGVGIPQASAKKEAA
                     YLYCQWAVSKTMGARLLQSGGGVPFRNSVLNDETVRKGVKMPPEWLDSVIGSAKISKL
                     GLPVIVPVAEFRDIVGAALTATLSGADPATELKKAHEQFRPILERSEKT"
     gene            3855..4754
                     /locus_tag="VN968_07755"
     CDS             3855..4754
                     /locus_tag="VN968_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXL29171.1"
                     /translation="MYLDAPPRLIETKVFSAMPDAYRRKGARTDWADANRRGQPTDCF
                     IEGPSFDTEGNLYIVDIPFGRIFKIAPDGKWLLVIEYDGWPNGLKIGPDGRILVADYM
                     HGIMEFDAKAGKMKPVLTARNSESFKGCNDLHIASHGEIYFTDQGQTGLHDPTGRVYR
                     LSPNGRLDCLTDTGISPNGLVLDPTETVLFVAMTRDNAVWRLPFMKDGSVSKVGRFCS
                     TFGASGPDGLTMDKAGRLFVAHASLGHVFVFAPNGELIARIKSCAGASCTNVAIGGPN
                     RDRLYVTESATGTVLSADISELR"
     gene            4751..5590
                     /locus_tag="VN968_07760"
     CDS             4751..5590
                     /locus_tag="VN968_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXL29172.1"
                     /translation="MKSKQFGNAGPQVSVVGQGTWYIDHGDRKTAVAALRRGVDLGMT
                     HIDTAEMYGDAELVVADAIAGQRDKVFLVSKVLPSNASRRGTITACERSLKRLNTDRL
                     DCYLLHWRGSYPLAETVAAFELLVSSGKIRSWGVSNFDDDDLEEMLAVAGKGRIACNQ
                     VLYHLRERAIEHAVIPWCEKHGVAVVAYSPFGHNDFPQPRSIAGEVLQRIAEAHGVSP
                     RQVALAFLTREPCMFAIPKASTPEHAAENAAAGDLVLSDEEIAGIDKAFPRGPKPRSL
                     PML"
     gene            5858..6799
                     /locus_tag="VN968_07765"
     CDS             5858..6799
                     /locus_tag="VN968_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXL29173.1"
                     /translation="MPLPERKSTARAAPARADRPFRGIALILASTVFLGVSDVTAKYL
                     SATLPSIEIAGIRFLVFALIMVPAMLPGSPLFALRTGRPGLQAMRGVALLLSSLFFIS
                     GLRFLPIAEASATGFVAPLFVTALSIVFLGENVGVRRWLATAAGLIGVLIILRPGSSA
                     FHPAAFFPIVSALAWACTLIMTRMMSGREHAITTMTWSSIVGVCVISALVPFVWVAPS
                     WHDILFGVLIGVASTAGQWIVVLAFRYADASVLAPFSYTQLVWVSLLGFVVFGEVPDV
                     WTVTGAAFIVASGLYTAHRERIRRSQLSAEAEPSPNA"
     gene            complement(6852..7223)
                     /locus_tag="VN968_07770"
     CDS             complement(6852..7223)
                     /locus_tag="VN968_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL29174.1"
                     /translation="MSIRRIVPNINSANPSASKPFYEKVLGLDPAMDMGWIITFASPS
                     NPTAQVSVVASDTRTEPHADISVEVADVDACHAAAQLQGYSIVYPLTDEPWGVRRFFV
                     RDPNGVIVNVVSHEAEPARPA"
     gene            complement(7233..>7640)
                     /locus_tag="VN968_07775"
     CDS             complement(7233..>7640)
                     /locus_tag="VN968_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXL29175.1"
                     /translation="GRITMQQLADRVGLSVSPCHRRVKLLEERGVITRYIATVDQKSL
                     GLHVSVFISIKLARQKEEDLNRFAKAISKWDEVLECYLMTGNRDYLLRVVVADLSSYE
                     AFLKNKLTRLDGIASIESSFALSQVKYSIALPV"
BASE COUNT         1419 a         2361 c         2419 g         1441 t
ORIGIN      
        1 ctatctgttc gccgacaacg ccgatctggc tcgatggcaa tcatggttgc gctcgaccgc
       61 cggcagcgat ctcgccgaat acaaggattt tggcggcggc gtctatcgcg cggcatcgtt
      121 tgccgaaaat cgcatcgcga catgcctgtt catcggtcct gcgcgtgatg cgggcgattg
      181 ggatgtcgtc aagaacctgt tcgcagcgga cgcgctcagc gacgatcagc gccggatgtt
      241 gctgtcgggg aaatcgaccg acgggctggc cagcgccggc cccatcgtct gcgcctgttt
      301 tggcgtcggc cgtaccgcga ttctcgcggc catcgcaggc ggcgcacgca cggccgccga
      361 gatcggtgta caattgaaag ccggcaccaa ttgcggctct tgcattccgg aattgaagcg
      421 ccttatagcg caggtgggcg cggccgcgcc ggtgcatgag caacacatgc tggctgcgac
      481 cgcgagttag cttcgtagcc cggatggagc gaagcgcaat ccgaactaca ggatggctcc
      541 ggatggctac ccgcccttga ccgcgccggc cgtcaggcct gtcacgatct ggcgctgcgc
      601 gaacaccgtt aatagtaaga ccggcaaggt gacgatcaac gccgccgccg cgagcgggcc
      661 ccagctcaac tggtcgaagg agatcatgtt gtagaccgca accggcagcg tgcgggtctc
      721 gcgccccgcc agcacgatgc caaagacaaa gttgttccag gagaagatca ccgccaggat
      781 aaacgcaact gcgattccgg gccgcgcgat cggcagtgcg acataacgga acacctgcca
      841 gcgggtggcc ccgtcgatca atgctgattc ttcgagctcc atcggcgtcg tttcgaaata
      901 gccgatcatg atccagatca cgatcggcac cgtcaccacc aggtggatga tgatctgcgg
      961 ccacagcgtt ccgagcaggc ccagccactg gaacaggaga aacagcggaa tcagatagga
     1021 cagccccggc gtgatgcgcg cgatcaggat caccaccgcc gccttgtggg cacgcatgcg
     1081 ggcgaggcca tagcccgcgg gcacgccgat cagcagcgcg aacgcggtcg cagcgccggt
     1141 gacgatcagg ctgttggtga aataggtcga gaaccgcttg gaatcgatga cggcggcata
     1201 gttgccccag ttgaaatgct cggggatgaa gaccggcgga tacgacgcgt tgtcgacctc
     1261 gaacttgacg gataacgaca acatccagag aaagaacagc accgccggcg acacgatcac
     1321 aatcagcgcg aagccgagtc cgatccggcc aagcaattgc cgtagcgggc tcatgcgatg
     1381 cctccggtat cgttccagtg cgtgctttgg cgcaagtgaa gcagcgcacc cgccagggcc
     1441 acgatgagcg cgaagaagat caccacgatc gcggacgcgt atccgacatc gtaatagacg
     1501 aaggcaacgc tatagagata gaggttgatc gtctccgagg cgctgccggg gccgccctgg
     1561 gtaatggcga agatgatgtc aaagctcttc accgcatcga tcatgcggat catcgacgcg
     1621 atgaaaagaa atggcatgat cagcggcagc gagatgtagc ggaacacctg ccagcgggtg
     1681 gcgccatcga tctgcgcgct ctcatagggc tcggccggga tcgcggcaag gccgccgagc
     1741 acgatcagca tcaccagcgg cgtccattgc caggtttcga ccaggaccag ggatgggatc
     1801 acggtcgcgg ggtgaaacac ccaaagctgc ggcggcaggc cgacgagcga cagcaggtag
     1861 ttgaggatac cgagttgcgg gtggaacatc atcgtccaca ccagcgcgat cgcgaccggg
     1921 gttgccatca tcggcaggat gaagaggccg cgcagaaagc cgcgcatcgg aaatttggcg
     1981 tgaaacacca ccgccgcgag cgtgccgaac aataacggca gcgcgaccga cagcgcggtg
     2041 tagaccagcg tgtgccacac cgactcgacg aaacgcgcat cgcccggcag gcggatgtaa
     2101 ttggcaagtc caacaaaggt cggcggcgcg ccgaccttcc attcgtgcag gctcatccag
     2161 atcgtgtagg cccacgggaa gatgatgacc gcaagaacca cgatcaacgc gggcagaacg
     2221 aatggccaat aggacggcgg ccgccatccg ccggtttcgc gcgaggcggc gcccgatcgg
     2281 gccggcgccg cttcgcttgc cgccaagccg ctcacgtttt ctcgctgcgc tccagaatcg
     2341 gtcggaactg ttcgtgcgcc ttcttcagct cggtcgctgg gtcagcgccc gacagcgtcg
     2401 cggtcagcgc ggcgccgacg atgtcgcgga actcggcgac cggaacgatc acgggaaggc
     2461 cgagctttga gatcttggcc gagccgatca ccgagtcgag ccattccggc ggcatcttca
     2521 cccccttgcg caccgtctca tcattgagca ccgaattgcg gaacggcacg ccgccgccgc
     2581 tttgcaagag tcgcgcgccc atggtcttcg ataccgccca ttggcaataa aggtaggcgg
     2641 cctccttttt cgcactcgcc tgcggaatgc cgacaccgtc gccataggcg gcggaatact
     2701 ggcctttcgg tccggccggc accagcgcat agccgatttt gccgaccacg cgcgacgcat
     2761 tgggatcctc gatcggcggg gcccagccga cgccgtcgat ccacatcgcc gcccgtccct
     2821 gcgtcagcga ggccatcgat tccatccagt tgaaaccggc gacgcccggt ggcgccgtct
     2881 tggtcaggag gcgctggtac atcttggtgg cctcgatcgc ctccgggcca tcggtgagga
     2941 tgttgccctt ggcgtcgaga aattcgccgc catagttgag gaagaaattg gtccacagcg
     3001 tcatgttggc attgcgcagg ccgcggccgg taaagccgta gatgccgttg gcggggtcgt
     3061 tgagcttttc ggcggcggtg gccatttcgt cgaaggtttt gggcagcgca acgcccttct
     3121 tcgcgaacag ctccttgttc cagtacaata tgaagtagtc gaccgaccac ggcagcgaat
     3181 gcatctcgcc cttgtcgttc tgggcaaatt gcagaccgcc ggcggaaaaa tcgtcgaccg
     3241 tgagatcggg cgcggtcagg ttcggatcct tcatgaaagg ggtcaggtcc gcgagccagc
     3301 cggccttttc gaattgccgc ttctgcacgt gataactgac gtgaatgacg tcaaagctcg
     3361 gcttgccgga ggtgagttcg atgacggctt tctgccgttg ctgctgctcc ggaatgactt
     3421 cggattcaac cttgatgcct gtgaggtcgg taaattcgaa ggcgtatttc tgcagcagct
     3481 cgccgcgcgg gcccttgatc aaattggctt cgatagtagt gccggcatat ttcttccact
     3541 gcggctcggc gcgcgccgga aatccggtga gactgagcgc gctcgcggcg agcgttccct
     3601 gcaataggga acgccgggtc gggccgaaat gagccatgtt gttatcctcc ctgcaatccg
     3661 cctttgaggc ggtgattatt tttgacgacg cgttcggcgg gccgccgagc gggtgtgcct
     3721 gtgcggattc atagcacgct gccgcctcaa agcatccggc tttttgcgga ggctgcccag
     3781 cgcgaggtga catcgacggc gcggacttgc tcatctatgg tgaccctctt cttttgcagg
     3841 tgaactcgcg aatgatgtat ctcgacgctc cgccgcggct cattgaaaca aaagtgtttt
     3901 cggcgatgcc cgatgcatac aggcgcaagg gcgcgcgcac cgattgggcc gacgccaacc
     3961 ggcggggcca gccgaccgac tgcttcatcg agggcccgtc attcgacact gagggcaatc
     4021 tctacatcgt cgatattccg ttcggccgca tcttcaaaat cgcgccggac ggcaaatggt
     4081 tgcttgtgat tgaatatgac ggctggccca acggcctgaa gatcggcccc gacggccgca
     4141 tccttgtcgc cgactatatg cacggcatca tggagttcga cgcgaaggcg gggaagatga
     4201 agccggtatt gacggcgcgg aattccgagt cgttcaaggg ctgcaacgac ctgcacatcg
     4261 cctcgcatgg cgagatctat tttaccgatc agggacagac cgggctgcac gatcccaccg
     4321 ggcgggtcta ccggctgtcg ccgaacgggc ggctcgactg cctgaccgac accggcatca
     4381 gccccaacgg cctggtgctc gatcccaccg agaccgtgct gtttgtggcg atgacgcgcg
     4441 acaacgcggt ctggcgcttg cctttcatga aagacggcag cgtttccaag gtcggccgct
     4501 tctgttcgac gtttggcgcc agcggtcccg acgggttgac gatggacaag gccggccggc
     4561 tgtttgtggc gcatgcgtcg ctcggacatg tgttcgtatt cgcccccaac ggcgaattga
     4621 tcgcgcggat caaatcctgc gcaggagcaa gctgcaccaa tgtcgccatc ggcggcccaa
     4681 atcgcgaccg cctctacgtg acggaatcgg cgaccggcac cgtgctatca gcggacatca
     4741 gtgagttgag atgaagtcaa agcagttcgg caatgccggc ccgcaagtgt ccgtggtcgg
     4801 gcagggcacc tggtacatcg accacggcga tcgcaagacc gcggtcgccg ccttgcgccg
     4861 cggcgtcgat ctcggcatga cccacatcga caccgccgag atgtatggcg atgccgagct
     4921 ggtggttgcg gatgcgatcg ccgggcagcg cgacaaggtt ttcctggtgt cgaaagtgtt
     4981 gccgagcaac gcgtcgcggc gcggcaccat caccgcatgc gagcgctcgc tgaagcggct
     5041 gaacaccgac cggctagatt gctatctcct gcactggcgt ggatcgtatc cgctggcgga
     5101 aacggttgcc gcgttcgagc tactcgtaag ctcagggaaa atccgttcat ggggcgtcag
     5161 caatttcgat gacgacgatc tcgaggagat gctggctgtt gccggaaagg gcaggatcgc
     5221 ctgcaaccag gtactctacc acttgaggga acgcgcgatc gaacacgcgg tgattccgtg
     5281 gtgcgagaag cacggcgttg cggtggttgc ctattcgcca tttggccaca atgattttcc
     5341 acaaccgcgc agcattgcag gcgaggtgct gcagcggatc gcggaagcac acggcgtatc
     5401 gccgcgccag gtcgcgctgg cgtttctgac gcgcgagcct tgcatgtttg cgattccaaa
     5461 ggcttcaacg cccgagcacg ccgcagaaaa cgccgcggcg ggagatctcg tgctcagcga
     5521 cgaggaaatc gcaggcatcg acaaggcctt tccgcgcggg ccgaagccgc gaagcctgcc
     5581 gatgctgtag cggacacgtt tcttaacaaa gtgtttcggc ggcgacggca agtggccgcg
     5641 ccggcaacgc caagtggccg cgccggcgac gatgagctac cgcgccgact gcggcggagc
     5701 gtcgcgcgca atcgcgcata tcggcttcct gttttcgggc attgtcgcgg gcggacaatt
     5761 gtcgttttta gaggttcacc cgattcgata atttctgccc ccgagttcac ccgtgacccc
     5821 gccccccgcc gcagccgcca ggctggaccg cgcttcattg cctctgcccg agaggaagtc
     5881 gaccgcccgt gccgcgccgg cgcgggccga ccggccgttc cgcggcatcg cgctgatcct
     5941 ggcatcgacc gtgttcctcg gggtctccga tgtaacagca aaatatctct cggcgacact
     6001 gccgtcgatc gagatcgccg ggatcaggtt tctggtgttc gcgctgatca tggttccggc
     6061 gatgctgccg ggttcgccgc tgttcgcgtt gcgaaccggg cgtcccggtc tgcaggcgat
     6121 gcgcggcgtc gcgctgttgt tgtcgtcgct gttcttcatc tccggcttgc ggttccttcc
     6181 aatcgcggaa gcatccgcca ccggtttcgt cgcgccattg ttcgtcaccg cgctgtcgat
     6241 tgtttttctc ggcgagaatg tcggtgtgcg ccgctggctt gccaccgcgg ccggcttgat
     6301 cggcgtgctg atcatcctgc ggccgggctc gagcgcattt catcccgccg cgtttttccc
     6361 gatcgtctcc gcgctggcct gggcctgcac cttgatcatg acgcggatga tgagcggcag
     6421 ggaacacgcc atcaccacca tgacctggtc gtcgatcgtc ggcgtttgcg tcatctctgc
     6481 gctggtgccg ttcgtctggg tcgcgccgag ctggcacgat atcctgttcg gagtcttgat
     6541 cggcgtggcg tcgacggcgg gacagtggat tgtcgtgctc gcctttcgct atgccgacgc
     6601 ctccgtgctc gcgccgtttt cctacaccca gctggtctgg gtcagcctcc tcggcttcgt
     6661 ggtcttcggc gaggtgcctg acgtctggac cgtgaccggc gccgccttca tcgtcgccag
     6721 cggtctctac accgcgcatc gcgaacgcat caggcgctcg cagctatcgg cggaggccga
     6781 gccgtctccc aacgcgtgat cggccgccgg cagcggcata ggtccccggc gttcgccggg
     6841 agcacggcct ctcacgccgg ccgcgctggc tcagcctcgt ggctcacgac attgacaatg
     6901 accccattgg ggtccctgac aaaaaatcgg cgaacgcccc acggttcgtc ggtcagggga
     6961 tatacgatcg aatacccctg cagttgagcg gctgcgtgac aagcgtccac gtcggccacc
     7021 tcgacactga tatccgcgtg cggctcggtc cgggtgtcgg atgccaccac gctaacctga
     7081 gcggtcggat tggagggcga agcgaaggtg atgatccaac ccatgtccat agccgggtcg
     7141 agcccgagaa ccttttcgta aaacggtttc gaagccgagg gattggccga gttgatgttg
     7201 ggtacaattc tgcggatgct catgggatgg tctcacaccg gcaatgcaat tgaatatttc
     7261 acttggctga gcgcaaaact cgattcgatc gaggcgatgc cgtcgaggcg cgtcagcttg
     7321 tttttcagaa acgcttcata cgacgacagg tcggcgacga cgacgcgcag caggtagtcg
     7381 cggtttccgg tcatcagata gcactccaga acctcgtccc atttcgagat cgccttggca
     7441 aaccggttga ggtcctcctc cttctgccgc gccagcttga tcgagatgaa cacgctgaca
     7501 tgcagtccga gcgatttctg gtcgacggtc gcgatgtagc gcgtgatcac gccgcgctct
     7561 tccagaagtt ttacgcggcg atgacagggc gagaccgaca ggccgaccct gtcggcgagc
     7621 tgctgcatgg tgatgcggcc
//
LOCUS       DATNOI010000202         6538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2543615,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000202 DATNOI010000000
VERSION     DATNOI010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543615"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="VN968_07780"
     CDS             <1..1329
                     /locus_tag="VN968_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXL29176.1"
                     /translation="DGMGVLAKIREAGLSIPVVVQTAHGGIDNVVSAMRAGAQDFVVK
                     PVGIERLQVSLRNALNASALKGELQRIRHSREGRLTFADIITRSGTMAGVLRTAQKAA
                     ASTIPVLIEGESGVGKELFARAIHGSGERRSKPFVAVNCGAIPDNLVESILFGHEKGA
                     FTGATERHTGKFVEASGGTLFLDEISELPLAAQVKLLRALQEGAVEAVGGRKPVKVDV
                     RIVSATNRKLLERVKSGQFREDLFYRLHVLPLTIPPLRMRREDIPHLLRHFLARFCAE
                     ENRLITGISGEAMTHLSQLEWPGNIRQLENAVYRAVVMSEGNELGLADFPQIHAQPQA
                     MPETHHNQVEPLVVGPAFHSTVPAMVSGNEIPIAPLPSGGTLAMLTRSGEMRPLEEME
                     TEIIRFAISHYRGQMSEVARRLKIGRSTLYRKLDEAAVDEQAADGRDGAN"
     gene            1638..3683
                     /locus_tag="VN968_07785"
     CDS             1638..3683
                     /locus_tag="VN968_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXL29177.1"
                     /translation="MRDRSTNRAGFDRVLMAVAATFLTVSATSALAQANPARSSAAEL
                     AIDAAIPRPDPVNLPPVSINDFKPDTTASVPEAATTVPAAAPAAATATPSVEPAAQPT
                     KAASTVAPADQPVADKLRDMLGTKSLRYFDRKAERAAVEKFYTTRDFAPLWTQAGSLN
                     ESGKGVVARLRDAASDGLNAADYPVPDFSAATTPDALAEADLKLTASMLDYARQAQSG
                     RMHWSQVSGDIQYPEHPIDPAEVLTNVTTAKDASAALDGYNPPQKLYRELKAKLAELR
                     GQGEGKVVPLWEGPALKFTPASKKQPVAELMEDARVPLLRAKLGITENADDAHYDAKV
                     AEAVRKFQAGAELKATGVLDDRTVKALNSPKRDRQIDTVIVNMERWRWLPRDLGAPSI
                     GDAYVILNIPDYTLKVMQHGAQVWATKVVTGKPGIHATPLLTETMKFITVNPTWNVPP
                     SIIYNEYLPALQQDPTVLDRMGLKLEHARDGSIHISQPPGEANALGRIRFNFPNKFLV
                     YQHDTPDKNLFTREERPFSHGCMRVQNPDQYAATLLNITAPNEHYTPEKIRGMYGHSE
                     IDIKFPTPIPVNITYQTAFVDDSGRLQLRKDVYGRDGTMLALLRNSRSKDLENVVAHS
                     QPNYVRPPGNLPTDVSFASDNTYSSGPNFFERLFGGPMAPPPVPSRRVPQQRRLFTR"
     gene            3951..5534
                     /locus_tag="VN968_07790"
     CDS             3951..5534
                     /locus_tag="VN968_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HXL29178.1"
                     /translation="MLAGFARQFNALSLSKAGYHFGLTSLLLLAGAGSVHDAAALNET
                     RTLSFHHTHSDEDLTVTFKRDGRYDEEALKQLNHYLRDWRSQDQTTMDRHLFDILWEV
                     YRDVDGKKPIQIVSSYRSPSTNAMLRRRSSGVARFSQHMLGHAMDFFIPDVPLEQIRF
                     AGLRLQRGGVGFYPTSGSPFVHLDTGSIRHWPRMTHDQLAHVFPDGRTVHVPSDGNPL
                     RGYDLALADIEKRGDGDGVSTKSKPGLFAALFKSKSNDEEDEGASAPVAGEAAAPAGA
                     MAASPADAVPMPRSKPKAGSTLQLASADAQIVQPAKSRQAAAASAEKAEPKPQTPADI
                     INARGFWGDAPTKPKQATPAQVAAISARRAVAAADPQPTASVPAAFEALAYAPPSNSP
                     VDRSNIVAASAPIPRSFRPARNPMAAPTIDQVVAKGPQGQDSAIATSTRIAAGTGNGV
                     WMRVMMLAPSASTSMSTTVLGDTDMTLMRIHFVKPQAVVAMSFSDDPQMGLVSDRFTG
                     PAVATLATQSFVMRTASLR"
     gene            complement(5722..5994)
                     /locus_tag="VN968_07795"
     CDS             complement(5722..5994)
                     /locus_tag="VN968_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HXL29179.1"
                     /translation="MATSAAAVKEEPATTFAKDQLKAIVERIERLEEEKKTISDDIRD
                     VYAEAKGNGFDVKALRTIVRLRKQDANERAEEETILETYMQALGML"
BASE COUNT         1275 a         2137 c         1978 g         1148 t
ORIGIN      
        1 gacggcatgg gcgtattggc caagatccgc gaagccggtc tcagcatccc cgttgtcgta
       61 cagaccgccc atggcggcat cgacaatgtg gtgtcggcga tgcgcgccgg cgcccaggat
      121 tttgtggtca agccggttgg catcgagcgg ctgcaggtgt cgctgcgcaa tgcgctgaac
      181 gccagcgcgc tgaagggtga actgcagcgc atccggcaca gccgcgaagg cagactgacg
      241 ttcgccgata tcatcacccg cagcgggacc atggcgggcg tgctgcgcac cgcgcaaaag
      301 gccgccgcct cgaccatacc ggtgctgatc gaaggcgaat ccggcgtcgg caaggaattg
      361 ttcgcccgcg ccatccatgg cagcggcgag cgcagatcaa agccgttcgt tgccgtcaat
      421 tgcggcgcca ttcccgacaa tctggtggag tcgatcctgt tcggccatga gaagggcgcc
      481 ttcaccggcg ccaccgaacg ccacaccggc aaattcgtcg aggcctccgg cggcacgctg
      541 ttcctcgacg aaatcagcga attgccgctg gccgcccagg tgaaactgct tcgcgcgctg
      601 caggaaggcg cggtcgaggc ggtcggcggg cgcaagccgg tcaaggtcga cgtccgcatc
      661 gtctcggcga ccaaccgcaa gttgctggag cgggtcaaga gcggacaatt ccgcgaagat
      721 ttgttctacc gcctccacgt gctgccgctg acgattcctc cgttgcggat gcggcgcgaa
      781 gacatcccgc atctgttgcg gcattttctg gcgaggttct gcgccgaaga aaaccgtctc
      841 attaccggca tcagcggcga ggcgatgact catctgtcgc aactcgagtg gcccggcaac
      901 atccgccagc tcgagaatgc ggtctatcgc gccgttgtca tgagcgaggg caacgagctc
      961 ggccttgcgg attttccgca aatccacgcg caaccccagg caatgccgga aacccaccac
     1021 aaccaggtcg agccgctggt cgtcggtccg gcgtttcact cgaccgtgcc agccatggtt
     1081 tcaggtaacg agatacctat tgcaccgttg ccgtcaggcg gaacgctggc gatgctgacg
     1141 agatcgggag aaatgcggcc cctagaagag atggagaccg agatcatccg gttcgcgatc
     1201 tcgcattatc gcgggcagat gtccgaggtt gcacgccgat tgaaaatcgg ccgctcgacg
     1261 ctttaccgca agctcgatga ggcggccgtc gacgagcagg cggccgacgg aagagacggg
     1321 gcaaactgag acaatgtcgg attgtgacac tccgctgttt gaaatgaagc ggaagacatc
     1381 ggcgcttcca gaacatttga accgttgctt gccggtgaca gacaaagcga agggcgcatg
     1441 gcaaaaacgc gcaatcggtt gcaatcggaa ccgcttgaag tcagtttgtc gtgagttgcc
     1501 cgcctgacgc tggctgcctg tcggtggcgc gtgtatcgtc tgcatgtgaa tcgttgcatg
     1561 ccggcgaaag ctgtgacgga cgatagacga tacaaaggct gttccatgac ggagcagttt
     1621 cgccaagggg tgtgacgatg cgtgaccgtt cgaccaaccg tgctggattt gaccgcgtcc
     1681 tgatggccgt tgcggcgact ttcctcacgg tgtccgcaac ctcggcgctc gcgcaggcca
     1741 atccggcccg cagcagcgcc gcggagctcg cgatcgacgc agcgatcccg cgtcccgacc
     1801 ccgtaaatct ccctcccgtg agcatcaacg atttcaaacc ggacaccacc gcgtcggtgc
     1861 ccgaggcggc gaccaccgta cccgcagcgg ctccggcggc ggccaccgca acgccatcgg
     1921 ttgaacccgc cgcccagccg accaaggccg ccagcaccgt cgcaccggca gatcagccgg
     1981 tcgccgacaa actgcgcgac atgctgggca cgaaatcgct gcgctatttc gatcgcaagg
     2041 ccgagcgggc cgcggtcgag aagttttaca ccacgcgtga ttttgcgccg ctgtggaccc
     2101 aggccggcag tctgaacgag agcggcaagg gcgtcgtcgc gcgtctcagg gatgccgcct
     2161 ccgacggttt gaacgccgcc gactatccgg tgccggattt cagcgcggcg accacgccgg
     2221 atgcgctcgc ggaagccgac ttgaaactga ccgccagcat gctggactac gcgcgccagg
     2281 cgcagagtgg ccggatgcac tggtcgcagg tcagcggcga catccagtat ccggaacatc
     2341 cgatcgatcc cgccgaagtg ctgacgaacg tcacgaccgc caaggacgca tccgcggcgc
     2401 ttgacggcta caacccgccg caaaagctgt atcgcgaatt gaaggccaag ctcgccgagt
     2461 tgcgcggcca aggcgaaggc aaggtggttc cgctttggga agggccggcg ctcaagttca
     2521 cgccggccag caagaaacag cccgttgccg agctgatgga agatgcgcgc gtgccgctgc
     2581 tgcgcgccaa gctcggcatc accgaaaacg ccgacgacgc tcattacgat gccaaggtcg
     2641 ccgaggccgt gcgtaaattc caggcaggcg ccgagctcaa agccaccggt gttctggacg
     2701 accgcaccgt gaaggccttg aacagcccca agcgcgaccg ccagatcgac accgtgatcg
     2761 tcaacatgga acgttggcgc tggctgccgc gcgatctcgg cgcgccctcg atcggcgatg
     2821 cctatgtcat tctcaatatt ccggactata cgctcaaggt gatgcagcac ggcgcgcagg
     2881 tctgggccac caaggtggtg accggaaaac ccgggatcca cgccacgccg ctgctgacgg
     2941 aaacgatgaa gttcatcacg gtcaatccga cctggaacgt gccgccgtcg atcatctata
     3001 atgaatatct gcccgcgctg cagcaggatc cgaccgtgct ggaccgcatg gggctgaagc
     3061 tcgagcacgc ccgcgacggc agcatccaca tctcgcagcc gcccggcgaa gccaacgcgc
     3121 tcgggcgcat ccgcttcaat ttcccgaaca agttcttggt ctaccagcac gatacgccag
     3181 acaagaacct gttcacgcgg gaagaacggc cgttcagtca cggctgcatg cgggtgcaga
     3241 atccggatca atacgccgcg acactgctga acatcacggc gccgaacgag cactacaccc
     3301 ctgaaaaaat ccgcggcatg tacggccaca gcgagatcga catcaaattc ccgacgccga
     3361 taccggtgaa cattacctat cagacggcgt ttgtggatga ttcgggcagg ctgcagctgc
     3421 gcaaggacgt ctacggccgc gacggcacca tgctggcgct gctgcgcaac tccagaagca
     3481 aggatctgga gaacgtggtc gcgcattccc agccaaatta cgtccggccg ccaggcaatc
     3541 ttccgactga cgtgagcttc gccagcgata acacttattc ttccggaccg aacttcttcg
     3601 agcggctgtt cggggggccg atggcgcctc ccccggtgcc gagccgccgc gtcccgcagc
     3661 aacggcggtt gttcacccgc tgattttcca ccgctttcgg cggcgggtcg gtatttcgca
     3721 gcaaaatcaa tggcctcgct atttcggcga ggccattttc attaaccatt ctccaccctt
     3781 ggttaaccag cggcactttt ttgccacact cgccgggtta ggttaacact aactttgggg
     3841 gatgggcgca aatcgacaaa ccctcccacg tcaaggctgc gttaatcctc tttcgccaca
     3901 cggggtagcg agagagttca tcgaacggtc gactctgggt gggctcatac gtgctggctg
     3961 gtttcgcacg ccaattcaat gcgctgtcgt tgtcgaaggc cggatatcat ttcggcctga
     4021 catcgctgtt gcttctggcc ggagccggat cggttcacga tgcggccgcg ctcaacgaaa
     4081 cccgcaccct ttcgtttcac cacacccatt ccgatgaaga tctcaccgtc accttcaagc
     4141 gcgacgggcg ctacgacgaa gaggcgctga agcagctcaa tcattatctc cgcgactggc
     4201 gcagccagga ccagaccacg atggatcgtc atttgttcga catcctctgg gaagtctacc
     4261 gcgacgtcga cggcaagaag ccgatccaga tcgtctcgtc gtaccgctcc ccctctacca
     4321 acgccatgct gcgccgccga tcgtcggggg tagcccgctt cagccagcat atgctcggcc
     4381 acgccatgga tttcttcatt ccagacgtgc cgcttgagca gatccgcttt gccgggcttc
     4441 gcctgcaacg cggcggcgtc gggttctacc cgacatcggg atcgcctttc gtccacctcg
     4501 acaccggcag cattcgccac tggccgcgaa tgacccacga tcagctggcc cacgtattcc
     4561 cggacggccg caccgtgcat gttccgtcgg acggcaatcc gctcaggggc tatgacctgg
     4621 ctctcgccga catcgaaaaa cgcggcgacg gcgacggcgt ttcgaccaag agcaaaccgg
     4681 gcctgttcgc cgctctgttc aagagcaagt cgaacgacga ggaggacgag ggcgccagcg
     4741 ctcccgttgc cggcgaagcc gccgcccccg ctggtgccat ggccgcttct cccgccgacg
     4801 ccgtgccgat gccgcgctcg aagcctaagg ccggttcgac cctccagctg gcttcggccg
     4861 acgcgcagat cgtccagccc gcaaaatcca ggcaggccgc cgcggcgtca gctgaaaagg
     4921 ccgaaccgaa gccgcagacg ccggctgata tcatcaacgc ccgcggtttc tggggcgacg
     4981 cccccaccaa accgaagcag gcaaccccgg cgcaggtcgc cgccatcagc gcccgccggg
     5041 cggtggccgc cgccgacccg caaccgaccg caagtgttcc ggccgcgttc gaggccttgg
     5101 cctacgcgcc gccatccaat tcaccggttg accgctcgaa tatcgtcgcg gccagcgcgc
     5161 cgataccgcg cagcttccgg ccggcccgca atccaatggc cgcacccacg atcgaccagg
     5221 tcgtcgccaa gggaccgcag ggtcaagaca gcgcgattgc gacatcgacg cggattgccg
     5281 cgggcaccgg caacggtgtc tggatgcgcg tcatgatgct ggcgccgagc gcgagcacgt
     5341 cgatgtccac gacggtactg ggcgataccg acatgacctt gatgcggatc catttcgtca
     5401 agccgcaggc cgtcgtcgcg atgagcttct cggacgaccc gcagatggga ctggtcagcg
     5461 accggttcac cggaccggcc gttgcgacgc tggcgacgca gtcattcgtg atgcgtaccg
     5521 catcgctgcg ctgagcgctg cgtcgaacct gaaccagttc cacaacttcg gcgtcgtcct
     5581 cgcgaacgcg aggacccata atccctgacc ctcgcaagag aggtttggaa ctggcccctc
     5641 ggattctctt gagcatcggc ggttatgagt cccggcattc gccgggacga cgagagcttg
     5701 agcgcttgcg cggctagcgc ctcacaacat tcccagcgcc tgcatatagg tttcgagaat
     5761 ggtctcctcc tcggcgcgct cgttggcgtc ctgcttgcgc agacgcacga tcgtgcgcag
     5821 cgccttgacg tcgaagccgt tgcccttggc ctcggcgtag acgtcgcgga tatcgtcgga
     5881 gatggtcttc ttctcctcct ccagccgctc gatgcgctcg acgatggcct tgagctgatc
     5941 ttttgcgaat gttgtcgcgg gctcttcctt gacggcagcg gcagaggtgg ccatcgggta
     6001 ctcctgaagg aactgacggg tgaatctgaa agagcaagcg ccgcgcttgg ccgcacgccg
     6061 attctcaaaa tgaatcttga aaagaccctc aagagtgcgg cgccatgcgt caaggcaaca
     6121 tcgcgctcat ccacagtgca cccacaggag aaagcatctg ttgtcgggtg aagcccgcga
     6181 tcccggcctt gtgccggcgt ttcgcggacg gcctcagcaa ggagcggttc cctgaccagc
     6241 accacaccag gaccagggtc cggagcactc gccagcgcgc gttttcttcg acaaggcgat
     6301 gccttttgtt agcgaggggt tagtgcttgc cgtccatctt cacggtttcg aacgcgtcgc
     6361 tgcgagccgc caccgggcgg acgtctgagt ttctttgagg aaggcacgga tgcagaccct
     6421 aggttccacc ctggacgagc agaaaggata cggccaaggc tttgattttc tgaggatatt
     6481 cttggctacc gccatcatcg cctggcacac cgcgagcctg accggccatc tggaaatg
//
LOCUS       DATNOI010000203         3837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_926939,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000203 DATNOI010000000
VERSION     DATNOI010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926939"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VN968_07800"
     CDS             <1..129
                     /locus_tag="VN968_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S16"
                     /protein_id="HXL29180.1"
                     /translation="YGPPEKQAMLEAPDLKTRAEILIAVTEMDLAKKRTSGDPPLQ"
     gene            217..807
                     /locus_tag="VN968_07805"
     CDS             217..807
                     /locus_tag="VN968_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXL29181.1"
                     /translation="MITSQIRYLTHPQVNIEPAVPVSSWGLSEVGRIRAQTLANAGSL
                     AGTTQIVCSGERKAIETAGIIAAKLNVAVEAREAMHENDRSATGFLDPDEFETVADRF
                     FAKPLVSIRGWERAIDAQLRIVREVELVLARDRAGDVLFVGHGAVGTLLFCHYSGVAI
                     DRAYDQPAGGGHYFAFVKHGRRVLHPWRRMECLLPA"
     gene            complement(1467..3050)
                     /locus_tag="VN968_07810"
     CDS             complement(1467..3050)
                     /locus_tag="VN968_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018096219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXL29182.1"
                     /translation="MQFVFEDYVLDLDRRELSRGSKAIATGPQVFDLLVYLVQNRAHV
                     VSKDNVLDAVWGGRIVSESTLTSHVNAVRKAVGDSGGEQRLIRTIARKGFRFVGDVSE
                     VQSSDGSSHANDESAGSNETPAPALALPDKPSIAALAFHNLSGDPEQDYFADGVVEDI
                     ITALSRIRWLFVIARNSSFTYKGRAVDVKQIGRELGVRYVLEGSVRKIANRVRITGQL
                     IDATTGAHLWAERFEGNLDDIFELQDQIAMSVVGAIAPQLERAEIERAKRKPTENLSA
                     YDYYLRGMANLHLGTREAIHEALSLFDEAIRLDPDFASAYAMAAWCHFWRKVNGWMID
                     RPRETAEGARLARRAVELGRDDAVALTRSGHALAHLAGDLEGGIALLDRALVLNPNLA
                     AAWFLGGFLRVWHGESDGAIEYFGRAMRLSPLDAETYRMQAGMAVAHLFAGRFDTASS
                     WAEKAFRDLPSFLMVVSIIAASHALAGRIDEGRRAMDHLRQLDPALRISNLRDWLPIH
                     RPEDLATFSDGLRKAGLPE"
     gene            3339..>3837
                     /locus_tag="VN968_07815"
     CDS             3339..>3837
                     /locus_tag="VN968_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018096220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL29183.1"
                     /translation="MKIVVIGGSGLIGTKLVNILRGKGHEVVAASPSSGINTITREGL
                     ADALIGAQVVVDVANSPSFEDKAVLEFFETSNRNLLAAEAAAGVGHHVALSVVGAERL
                     PESGYLRAKMAQEKLIKASGIPYTILRATQFFEFVDSIIKSGTAGGVIRLSPALLQPV
                     ASDDVV"
BASE COUNT          724 a         1219 c         1188 g          706 t
ORIGIN      
        1 tatggcccgc cggaaaaaca ggcgatgctt gaggcgcccg atctcaagac ccgcgccgag
       61 atcctgatcg cggtgaccga aatggatctc gcgaagaagc gcaccagcgg cgacccgccg
      121 ctgcaatgag attttacgat cgtcattccg gggcgtgagc gaagcgaaac gaacccgaaa
      181 tgacggcttg gaaattgcga ttgccgcatc atccagatga taaccagcca gatccgctac
      241 cttacccacc cacaggtgaa catagagccg gctgtgccgg tctcgtcatg gggcttgagc
      301 gaggtcggaa gaatacgcgc gcagacgctg gcgaacgccg gatcgctcgc cggcacgacg
      361 cagatcgtct gcagcggcga gcgtaaggca atcgagacag ccgggatcat tgctgcaaaa
      421 ttgaacgtcg ccgtcgaagc gcgggaggcg atgcacgaga acgatcgatc ggcgacaggt
      481 ttcctagacc cggacgagtt tgagacggtc gccgatcggt ttttcgcaaa accgctcgtc
      541 agcatccggg gatgggagcg cgccatcgat gcccagttac gtatcgtccg cgaggtcgaa
      601 ctcgtgctgg cccgggatcg cgcgggcgac gtgctctttg tcggccacgg cgccgtcggc
      661 accctgttgt tctgtcacta ctccggggtc gcgatcgatc gcgcctatga tcaaccggcg
      721 ggcggtggtc attacttcgc gttcgtcaag cacgggcggc gcgtgctgca tccgtggcgc
      781 cggatggaat gcttgcttcc tgcgtgaggc atcgactcga tcatgatccg attagattga
      841 atcggatcat gatctcatcc tttcgttgga gcatgatctt ttcggaaaac cggttcccgc
      901 ccatcgggtc gtggcccgag ggcatgcttt tccggatcat gctctgacag cagcggcgct
      961 ctgatggttc caccaaaaac cctcatggtt ccgcaaaaaa ataagtccct gggcgagcac
     1021 ctaagcactc gaaagggcgg actgcggctc tcgggcagct taagttacaa ccatgccgtg
     1081 tcactgattc gcagtgcacg caacaggccc ggcaagttgc cagcgcctgc gaaggataag
     1141 gcggccgcct cgatcacaaa tgcagccgac actttcgccc gatgatgttt gccgttcgcg
     1201 ttgtgcggcc attcgcgagt gcttctgcgt ttgaaggctt gattctgtag caattgaaat
     1261 tcggcgacct ggcaagagcg accctctgcc gtacccgtag agggtgggcc gcgtcgctgt
     1321 ccggagcccg cgttgggcgc ttcctcccag actgcccggc ggccgagcgc cgccgggcac
     1381 cttttcggtc agcgggtatc ccatgccgcc gcaattccgc cagcgcaacc gccgcgcgcc
     1441 gcgccacccg cccgaaacgc cgattgtcat tccggcagcc cggcttttcg caggccgtcc
     1501 gagaacgtgg cgagatcttc cggtcgatga atcgggagcc aatccctgag attggagatg
     1561 cgcagcgcgg gatcgagctg acgcaaatga tccatcgccc ggcggccttc gtcgattcgg
     1621 cccgccagcg cgtggcttgc cgcgataatg ctgaccacca tcagaaagct tggcaaatcc
     1681 cggaacgcct tctccgccca tgacgacgcc gtgtcaaagc gccctgcgaa gagatgcgcc
     1741 acggccatgc cagcctgcat tcgataggtt tccgcgtcga gcgggctcag acgcatggcc
     1801 cgcccgaaat actcaatggc gccgtcggac tcgccgtgcc agaccctcag aaatccaccg
     1861 agaaaccagg cagccgccag gtttggattg agcaccaggg ctcggtcgag caaagcgatg
     1921 ccgccttcga ggtcgccggc aagatgggcg agcgcgtggc cgcttctcgt gagtgcgacc
     1981 gcatcgtctc tgcccaactc gacggcccgg cgcgccaatc gagcaccctc ggcggtctcc
     2041 cgcgggcgat cgatcatcca gccgttgacc ttgcgccaga aatggcacca cgccgccatc
     2101 gcataggccg acgcgaaatc cggatcgagc cgtatcgcct catcaaacag tgacagcgcc
     2161 tcgtggatgg cctccctggt tcccagatgc aggttggcca ttccgcgcaa ataatagtca
     2221 taagcgctga gattttcggt cggcttccgc ttggcgcgtt cgatctccgc acgctccagc
     2281 tgcggcgcga tcgcgccgac gacgctcatg gcgatctggt cctgcagttc gaagatgtcg
     2341 tcgaggttgc cttcgaaccg ctccgcccag agatgcgccc cggtggtcgc gtcgatcaat
     2401 tgccccgtga tccgcacccg gttggctatc ttgcggacgc tgccttccag cacatagcgt
     2461 acgcccagtt cgcggccgat ctgcttcacg tccacggcgc ggcccttgta ggtgaagctc
     2521 gaattgcgcg cgatcacgaa cagccagcgg atgcgcgaca atgcggtaat gatgtcttcc
     2581 acgacgccgt cggcgaagta atcctgctcg gggtcgccgc tcagattgtg gaacgcaagg
     2641 gcggcgatcg acggcttgtc gggaagcgcc agcgcaggtg caggcgtttc atttgacccg
     2701 gcggactcgt cgttggcgtg gctggagcca tccgatgatt ggacttcgct gacgtcgccg
     2761 acgaaccgga atcccttgcg ggcgatggtc cggatcaggc gttgttcccc gccgctgtca
     2821 ccgaccgcct tgcgcaccgc attgacgtga ctggtcagcg tcgactccga gacgatgcgg
     2881 ccgccccaca ccgcgtccag cacattgtcc ttgctgacga cgtgcgcgcg gttctgcacg
     2941 agatagacca gcaggtcgaa gacctgcggc ccggtcgcaa tggccttcga cccccggctg
     3001 agctcgcgcc ggtcaaggtc gagcacataa tcttcgaaaa caaattgcac gtcggcggcg
     3061 ctccatcacc tcaggcgcag tgccgggatc gatttcgtcg cggccgggct gacagttgtt
     3121 ccggccggac aagcccttga tagcacggcg gtcctcgggg gccaccgttc gcggcgcgac
     3181 cgtttgtcag cggccaggcg gtgggatagc ggcccgcttg gacaaataag caaggaaaac
     3241 acaatgccaa ttcaaggccg ctgcaaagtc ttccggccgg cccggaggca ttctcccatc
     3301 accagcccga tgggcgggcg gcaaacggga gagaaattat gaagatcgtg gttatcggag
     3361 gcagcggcct gatcggaacc aagctcgtga acattcttcg aggcaagggc catgaggtgg
     3421 tagcggcatc gcccagttcg ggcatcaaca ccatcacgcg cgagggactg gccgacgcgc
     3481 tgatcggcgc gcaggtggtc gtcgacgtgg ccaactcgcc gtcgttcgag gacaaggccg
     3541 ttctggaatt cttcgaaacc tcaaatcgaa accttctcgc cgcggaagcc gccgcgggcg
     3601 tcggacatca cgttgcattg tcggtggtcg gtgccgaacg ccttcccgaa agcggttacc
     3661 ttcgcgcgaa gatggcgcag gagaagctga tcaaggcttc cggcattccc tacacgatcc
     3721 tgcgcgcgac gcagttcttc gagttcgtcg acagcatcat caagtcgggt accgccggcg
     3781 gtgtcattcg gctgtcgcca gccctgctgc agccggtggc atcggacgac gtcgttg
//
LOCUS       DATNOI010000204        11742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_917984,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000204 DATNOI010000000
VERSION     DATNOI010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11742
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917984"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..1222
                     /locus_tag="VN968_07820"
     CDS             254..1222
                     /locus_tag="VN968_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL29184.1"
                     /translation="MGSWFRIVAAAVAMSAAGGGAAAQSPFPSKPVHIFVPYAAGGGV
                     DILARTLGDVVSRQWGQTVVVENRPGAGGVVASQALVASPPDGYTLIVVASGHATNAF
                     LYPKIPYDTFKDFTPISLLASSPNILLVRTDSPFKTLGDMIAQARSKPGSLSFGHAGT
                     GTSTHLAGELLKNLAKIDLNAIPYKGGAPSINDLLGGQIPMSFNNGPESVGQLEAGTV
                     RALAVTTASRASFLPNVPSMAEAVPGYDTEVWWGLLGPAGMPADLIAKLSHDFVAALN
                     TDPVKERLGKLGAPPIGSSPSEFDAKIRADASKWGPIIEAAGMKAE"
     gene            1219..1704
                     /locus_tag="VN968_07825"
     CDS             1219..1704
                     /locus_tag="VN968_07825"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL29185.1"
                     /translation="MTPMIPACLAPRDVSRPKVSPPPNACDTHAHVFGPAARFRYADD
                     RSYTPPDAPLEKYLDMLDSIGFARGVLVQGSAHGRDNSAMLDALAHRPDRLRPFHEAL
                     VRANPERLVWGGDWPHPRVEGEMPDAGHLFELFQLWTPDQATQHRILVTNPAKLYDFP
                     N"
     gene            1718..2767
                     /locus_tag="VN968_07830"
     CDS             1718..2767
                     /locus_tag="VN968_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HXL29186.1"
                     /translation="MSVNNKRVFYVKYLAHEIYTDILRKRPDVRLDRLENESAEAISA
                     PILAAAHAYQVGAARDELAPQFHVDRDLLRRAPNLLIVSSNGAGYDPVDVEACTEAGV
                     VVVNQSGGNARSVAEHALGMLLTLSKRILEADRALRRDPNVNRNALIGTEAQGKTIGI
                     VGLGNVGRRIAELCSGLLGMKVLACDPYLDATEMAARGGEKVELDDLLSRSDYVSISC
                     PLTKDSRGMIGARQFALMQPHAYFITTARGLIHDEAALVEALRDKRIAGAGLDVWARE
                     PPPPDHPLLQFDNVLASPHTAGVTKEARANMGRIAAEQILDALDGKRPPRLINPEVWP
                     RYAKRFERTFGFAPG"
     gene            complement(3246..3914)
                     /locus_tag="VN968_07835"
     CDS             complement(3246..3914)
                     /locus_tag="VN968_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXL29187.1"
                     /translation="MSSTGPKQAIFTSLAEVAQALGHAHRLELLEHIAQGERSVEELS
                     ARANLSFANASRHLQILRRARLVDTERRGKRVLYRLAGDNEVVDLMRALGRVGERNAA
                     EVGRIMIDYFHARDSLEAVSRKDLVSRLRDGLVTVLDVRPEDEFALGHLPGALNIPLS
                     KLEHRLSELPADREVIAYCRGPYCVLSFEAVAALRARGYLVRRLEDGYPEWKAAGLPI
                     EAEA"
     gene            3987..4727
                     /locus_tag="VN968_07840"
     CDS             3987..4727
                     /locus_tag="VN968_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL29188.1"
                     /translation="MIIRQFLHQEPVGISYLFGCGGRAAGAAVDPVGEIEPYMRAAEV
                     AGMRIHFVIDTHVHADHLSAGRQLAKAAGAEYVLAAHAQVSFPFRAVCDDEVLPLGNV
                     AAKVLHTPGHTPEHICILVSDRTRADEPWFVLTGHTLMVGDLGRTELAVSAERGAMDL
                     FQSARRLKALPDYVEVLPGAYAGSVCGRRLSGKPWSTIGFEKRHNEALGIGEEAEFIR
                     FMLADIPPAPPEAAVLRATNSGATAAAG"
     gene            4731..5993
                     /locus_tag="VN968_07845"
     CDS             4731..5993
                     /locus_tag="VN968_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL29189.1"
                     /translation="MSEAASALQSEDSRVRLGLKENWRQFALLIVINAFVGGMVGLER
                     TVVPLIGSEEFGIASTTLVVSFIVSFGVIKALANLVSGQLADAWGRKRVLVLGWLVGL
                     PVPFMIIWAPSWGWIIAANALLGINQGLAWSMTVIMKVDLVGPKSRGLAVGLNEFAGY
                     LAVGVTAFLTGYLASRYGLRPVPIYLGIGYAVLGALLSIMLVHDTRDHVRLEAAASSR
                     QSTPMSFREIFVLTSFRDRNLFAASQAGLVNNLNDGMSWGIFPLFFASFGLGVERIGI
                     LKAVYPATWGILQIATGPLSDRWGRKGLIVVGMWIQSAGLFVTAATRQFEWWLVGSLL
                     LGLGTAMVYPSLIAAVSDASDPTWRARSLSVYRFWRDLGYAIGALSAGIIADLFGIAW
                     AIGIIGALTFSSGTIVAAVMTSRSGREP"
     gene            5990..6379
                     /locus_tag="VN968_07850"
     CDS             5990..6379
                     /locus_tag="VN968_07850"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXL29190.1"
                     /translation="MSEIYSGRCLCGAVRFEATGKPKWVRWCHCESCRRHSGAPVSAF
                     VSFENDAVTITEGVITKYNSSPGVERGFCARCGSTLTCANVRLPTETHYYVGAFERAA
                     ELKPTGEFFAGERLPWVHTQDGSSAIE"
     gene            6399..7556
                     /locus_tag="VN968_07855"
     CDS             6399..7556
                     /locus_tag="VN968_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HXL29191.1"
                     /translation="MKTFNDPPYCTERVYLDYNASTPIDPAVAAAMKPFLADHFGNPS
                     SGHWAALPAKTALEEARRQIAALLGCEDDEVVFTSGGSEANNLALKGVYFARRDSGEH
                     IITTRIEHPAIVEPCRFLERLGARITYLPVDRYGRVDPGDLGQAITPRTILISIMHAN
                     NEVGTIQPIEECARIAREHGVLFHTDAAQSVGKIATDVNGLGVDLLSIAGHKLYAPKG
                     IGALFIRRGVTLEPHNHGAGHESGRRAGTESAMLAVGLGKACELARDLATIDRVRVLR
                     DHFWRELQERFDDRITLNGHPAHRLPNTLNVSFIDHVGVDVLERLDGVAASTGSACHA
                     GRVELSPVLEAMGVALEVGMGAIRFSLGRGTTDEEIEAVVERLNAAIAVAP"
     gene            7776..8867
                     /locus_tag="VN968_07860"
     CDS             7776..8867
                     /locus_tag="VN968_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXL29192.1"
                     /translation="MFEIDASFRPAAATLALLTAFLLLRDARFEFRARLGALFGIGTA
                     AYMFCSGAPGVWRASSLLLPLCIGNSVFFWWFALALLEDDFRLEAVHVGVLIALLALG
                     FARVGAHIIEYGALGQALAIFHNLVILTLVAHVLLLAWRGYDNDLLENRRRFRLLFLI
                     GGSLTAVGIAVAEVALAGRPPAAWMLALQSVLVSILATSAAAWLLSASASRLAFQNIT
                     TAKRAEPAPVMDRQRLIDEALGVALERAINIDKLYLEQGLTIAALAKRLGVPEHRLRK
                     HINEALGHSNFNAYVNQHRIAAAKAALRDPKLAHLPVLTIALDSGFASLPPFNRAFRA
                     ETGQSATAYRSAALVETRSANGRIESEKS"
     gene            8982..9797
                     /locus_tag="VN968_07865"
     CDS             8982..9797
                     /locus_tag="VN968_07865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HXL29193.1"
                     /translation="MIQFTSILDEFRQIWPYIFAGDLARYLIGAGSVFLLIWVIFRSR
                     LAARKIRGNTPPAGQIWREIGHSLMTVCIFATVGTAIGIGAMHGVFQVYADISLHGWL
                     YLVGSVVAMIVVQDTYFYWVHRLMHRAPLLWKVHATHHRSHNPTPWTAYAFDPGEALI
                     HALFMPLFVAVVPMHVSGLFAFTAHMMLRNAIGHCGYELFPKGWADRPILGLVTMVTH
                     HDMHHEHAPRNFGLYFTWWDRFMGTEHPEYRTRTKLVTPDHEMPDNLKGTYAS"
     gene            9880..10524
                     /locus_tag="VN968_07870"
     CDS             9880..10524
                     /locus_tag="VN968_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXL29194.1"
                     /translation="MNLYERWVLPPILDLIMRQKHLTKYRRAVVAVARGRVLEIGVGS
                     GLNFPLYGKEVELVYGIDPSRRLLSIARRRAAASAVPVELHLGSATAIPLADDTVDTV
                     VMTWTLCSIPDPLAALREMRRVLKPSGNLCFVEHGLSPEPSVEHWQRRITPAWRRVAG
                     GCHLDRKIDELIRLAGFDLTNLQVEYAPGPRAMSYMYEGCACRTHSGGIGPAGA"
     gene            complement(10621..10995)
                     /locus_tag="VN968_07875"
     CDS             complement(10621..10995)
                     /locus_tag="VN968_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXL29195.1"
                     /translation="MLKSKTAVLKPVGLTLAQYVALAELEQKQGITAATLARACFVSP
                     QAIMILLKTMEQQGLIARQPHPRHPNVLELHITDVGREALHAGRAQVAPVEDRIFAAF
                     SKKELEAFRAFLSRFVSAIDAG"
     gene            complement(11064..>11742)
                     /locus_tag="VN968_07880"
     CDS             complement(11064..>11742)
                     /locus_tag="VN968_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29196.1"
                     /translation="VLDVSDREAVERFAGAIADRHGGVDIVFSNHYARVQPGENPAEV
                     IDGYVAANNLGTTHILRNFAPLIRDNGRLLVVASRAGSLRALAPALHGRFENLQTLDD
                     VDRAVCAWRDIVRQGLAAGQAWPEWINIPSKIGQVAAVRVLAQQRRAEDLRRGILIAA
                     ISPGLIDTGASRQWLDMTGAPPPDEVAIPLLDLALEAAPDAAFYGGLINLGRGEPGRF
                     RTLVPWI"
BASE COUNT         2136 a         3618 c         3592 g         2396 t
ORIGIN      
        1 tcgtgccagg ccttctcttg aaaggcctgg ggcaggtttt gtccgtcggc agccgccatc
       61 gaattctccc ttatctcgcg acgatttttc gttcgcgacg tgatcgcggc cgtgttgtcg
      121 aggcgcggaa acttgacgcg atctgcggga ttgtcaacat gttcggatcg taccggtgat
      181 ctgcgcttca cggtggtgcc aacggtacgc cggcgtgcga taaaataaac gagaatatcg
      241 ggaggggcat gccatgggca gctggtttcg gatcgttgcc gcggcagtgg cgatgtctgc
      301 cgcgggtggt ggtgcggctg ctcagtcgcc attcccgtcc aaaccggtgc atattttcgt
      361 gccctatgca gccgggggcg gcgtcgacat cctggcgcgc acgctgggtg atgtggtctc
      421 ccggcaatgg ggccaaaccg tcgtggttga aaaccggccg ggggcgggtg gggtggtcgc
      481 gtcgcaagcg ctggtggcgt cgccgccgga tggctacacc ctcattgtcg tggcgagcgg
      541 tcacgccacc aatgcgttct tgtaccccaa aattccctac gacaccttca aggacttcac
      601 gccgatctcg ctgctggcgt cgtctcccaa tatcctgctg gtgcgcaccg attctccgtt
      661 caagacattg ggcgacatga tcgcgcaggc gcgctcgaaa ccgggcagcc tgtccttcgg
      721 ccatgccggg accggaacat cgacgcatct cgccggcgag ttgttgaaaa atctcgccaa
      781 gatcgatctc aatgcgattc cctataaggg cggtgcgccc tcgatcaacg atctgttggg
      841 cgggcaaatt ccgatgtcct tcaacaacgg cccggaatcg gtcggacagc tcgaagccgg
      901 taccgtccgc gcgctcgccg tcacgaccgc ctcgcgggca tcgtttctgc ctaatgtgcc
      961 cagcatggcc gaggccgtac cgggctacga cacggaagtg tggtggggcc tgctcggccc
     1021 ggccggcatg cctgccgacc tgatcgcgaa actctcgcat gatttcgttg cggccttgaa
     1081 cactgacccg gtgaaggaac gtctgggcaa gctcggcgcg ccgccgattg gcagttcgcc
     1141 ttcggaattc gacgccaaga ttcgtgccga tgccagcaaa tgggggccca tcatagaggc
     1201 cgccggcatg aaagccgaat gacgccgatg attcccgcct gtctggcacc gcgcgatgta
     1261 agccggccga aagtatcgcc gccgccgaac gcctgtgaca cgcatgcgca cgtgttcggg
     1321 ccggcggcgc ggtttcgcta tgcggatgat cgcagctaca cgccgccgga cgcgccgctc
     1381 gaaaaatatc ttgatatgct cgacagcatc ggctttgcgc gcggcgtgct ggtgcagggc
     1441 agcgcgcacg gccgcgacaa ttccgccatg ctcgacgcgc tggcgcatcg accggaccgc
     1501 ctgcggccat ttcacgaagc gctggtgcga gccaatccgg aacgtctggt gtggggcggc
     1561 gactggccgc atccgcgcgt cgaaggcgag atgcccgatg ccggacacct gttcgaattg
     1621 ttccagctgt ggacgccgga ccaggcaacg cagcaccgca tcctcgtgac caatccggcc
     1681 aaactctatg atttcccgaa ctgaaggctc gcaataaatg tccgtcaaca acaagcgtgt
     1741 gttttacgtc aaatatctgg cgcatgagat ctataccgac atcctgcgga agcgacccga
     1801 cgtgcggttg gaccggctcg agaatgaaag cgccgaggcg atctcggcgc cgatcctggc
     1861 cgcggcgcac gcctatcaag tcggtgcggc gcgcgatgaa ctcgcacctc agttccatgt
     1921 cgaccgcgac ctgctgcggc gcgcgccgaa cctgctgatc gtctcgagca atggcgcggg
     1981 ctacgatccc gtcgatgtcg aggcctgcac cgaagcaggc gtggtggtcg tcaatcagtc
     2041 cggcggcaat gcccgctcgg tggccgaaca cgcgctcggg atgctgctga ccttgtcgaa
     2101 gcgcattctc gaggctgacc gcgcgttgcg gcgcgacccc aatgtcaatc gcaacgcctt
     2161 gatcggcacc gaggcgcagg gcaagaccat cggcattgtc gggctcggta atgtcggccg
     2221 ccgcatcgcc gaactctgca gcggcctgct cggcatgaag gtgctggcct gcgatcctta
     2281 tcttgacgcg accgagatgg cggcgcgcgg cggcgagaag gtcgaactgg acgacctgct
     2341 gtctcgttcc gattacgtgt cgatctcttg cccgttgacg aaagacagtc gcggcatgat
     2401 tggcgcgcgc caattcgcgc tgatgcagcc gcatgcctat ttcatcacga cggcgcgcgg
     2461 ccttatccac gatgaggccg cgctggtcga ggcgctgcgc gacaaacgca tcgccggggc
     2521 cggcctcgac gtctgggcca gggagcctcc gccgccggac catccgctgc tgcagttcga
     2581 caatgtgctg gccagcccgc atacggcggg ggtgaccaag gaggcgaggg cgaacatggg
     2641 ccgaattgcc gccgagcaga ttctcgatgc gctcgatggc aagcggccgc cacgtctcat
     2701 caatcccgag gtttggcccc gctacgccaa acgcttcgag cggaccttcg gatttgcgcc
     2761 gggatagggg ctcggaccgc tgcgccgggc agcatgcggg agattttcga gagagaagat
     2821 ttcgaccgtt tgcggctgga tgccagcaat aaatctactg tgaatcaacc gccggacgaa
     2881 tggcacgttg ctattttcgc cgcgccctcg acacctgtcc gggccgggac cacgtttctt
     2941 ttgtgcgctt tttcagggaa cgcttgtttc cgcgggcgca aaaggaagct gcgatatgcg
     3001 tcggataggt tcattgttgc gcgctgcgcc atcccggatc gggcgccgtc tggcgctgat
     3061 cgtcgccatt gcggcggcaa gcctgccttc ggccggcgca tagaccgcgc cacgcgacta
     3121 gaggatcgac ggccccgctg gcttcctttc aggactgcca cttcccttcg ccataacggc
     3181 tgaatgtttg ttcgcgtcgc tttcgcgcgt agtggcgtca acggcatccg tcgatgttgc
     3241 tccgttcagg cctcggcttc gatcggcaaa cccgcagcct tccattcggg ataaccatcc
     3301 tcgagtcgac gaacgagata gcctcgcgcg cgaagcgctg cgaccgcctc gaacgagaga
     3361 acgcaatagg gaccgcggca ataagcgatg acctcgcggt ccgccggcaa ctcgctcaag
     3421 cgatgttcca gtttgctaag cgggatgttg agggcgccgg gaagatgccc gagcgcaaac
     3481 tcgtcttcgg gccgcacgtc gagaaccgtc acaaggccat ctctgagccg agagaccaga
     3541 tcctttctcg acacagcctc cagcgaatcg cgagcatgga agtagtcgat catgatgcgg
     3601 ccgacctcgg ctgcgttacg ctcgcccaca cgtccgagcg cccgcatcag gtcaaccacc
     3661 tcattgtcgc cggcgagccg atataacacg cgcttcccgc ggcgctcggt atcgacaagc
     3721 cgcgcgcgcc gaagaatctg caagtgtcgc gacgcgttgg cgaagctcag gttggcccgc
     3781 gcagaaagtt cttcgacgct gcgctcgcct tgggcgatgt gctccagcaa ctccagccga
     3841 tgcgcgtgac caagcgcctg ggccacctcc gcaaggctgg tgaaaatcgc ttgcttgggt
     3901 ccagtgcttg acaccgcttc ctctcagtga aacattcagc agaatgattg aatatattta
     3961 gctcgtgaag tgcgggagcg caagccatga tcatccgtca attcctgcat caggaacccg
     4021 ttggtatctc atatcttttc ggctgcggtg gacgtgcggc cggtgccgcc gtcgatccgg
     4081 tcggcgagat cgagccttac atgcgcgccg cagaggttgc cggtatgcgc atccattttg
     4141 tgatcgacac ccacgttcat gccgaccatc tctcggcagg ccggcagctc gccaaggcgg
     4201 ccggcgctga gtatgtgctc gcggcgcacg cgcaggtttc gtttccattc agagcagtgt
     4261 gcgacgacga ggtgcttccg cttggtaacg tcgccgcgaa ggtgctgcat acgccgggcc
     4321 acacaccaga acacatttgt atcctcgtca gcgatcgcac ccgggccgat gagccctggt
     4381 tcgtgctgac cggacacact ttgatggtcg gcgacctcgg ccgcacggag ctcgccgtca
     4441 gcgccgaacg aggagcgatg gacctgttcc agagtgcgcg ccgactgaaa gcgctgccgg
     4501 actatgtgga ggtgctgccc ggagcttatg ccggctcggt ctgcggccgg cgtctcagtg
     4561 gaaaaccctg gtcgaccatc ggcttcgaga aacgccacaa cgaggcgctc gggatcggag
     4621 aggaggccga gttcattcgc ttcatgctcg ctgacattcc gccggcccca cccgaggcgg
     4681 ccgtattgag ggccaccaac tccggtgcga cggccgcggc aggctgaccg gtgagcgaag
     4741 cggcatcagc tctacagtca gaagactcgc gtgtcaggct cgggctcaag gaaaactggc
     4801 gacagttcgc gctgctgatc gtgatcaatg cctttgtcgg tggcatggtt gggctggaac
     4861 gaacagtcgt gccgctgatc gggtcggaag agttcggcat tgcctcgacg acgctcgtgg
     4921 tctccttcat cgtcagcttc ggcgtgatca aagcccttgc caatctcgtc tcaggccaac
     4981 tcgccgatgc ctggggccgg aagcgtgttc tcgtcctcgg ctggctggtc gggctgcccg
     5041 tccccttcat gatcatatgg gccccgagtt ggggatggat cattgcggcc aatgcgctgc
     5101 tcggcatcaa tcaggggctt gcctggtcga tgacggtgat catgaaggtc gatctggtcg
     5161 ggccgaagtc gcgcggcctc gccgtcggcc tcaacgagtt tgccggctac cttgccgtag
     5221 gtgtcacggc attcctcacc ggctatctcg cctctcgata cggccttcgg ccagtgccga
     5281 tctatctcgg catcggctat gcggtcttgg gggccttgct gtcgatcatg ctggtccacg
     5341 acacgcgtga tcacgtccgg ctcgaagccg ctgcgtcttc gcggcaatca actccgatga
     5401 gcttccggga aatcttcgtg ctcacctcgt tccgtgaccg caatctcttc gccgcgtcgc
     5461 aagctggcct cgtcaacaac ctcaacgacg gcatgagctg gggcatcttc ccgctgttct
     5521 ttgcctcctt cggcctcggc gtcgagcgca tcggcatcct gaaggcggtc tatcccgcga
     5581 catggggaat cctgcagatc gccaccggcc cactcagcga tcgttggggc cgcaagggcc
     5641 tgatcgtcgt cggcatgtgg atccagtcag cgggcctgtt cgtcaccgcc gcaacgcgcc
     5701 agttcgaatg gtggctcgtc ggcagtcttc tgctcggact aggaacagcg atggtgtacc
     5761 cgagcctgat cgcggccgtc tccgacgcct cagatccgac gtggcgcgct cgctcgctca
     5821 gcgtctaccg cttttggcgc gacctcggct acgcgatcgg cgcactgtcg gcgggcatca
     5881 tcgccgattt attcgggatc gcctgggcga tcggcattat tggcgcgctc accttttcat
     5941 cggggaccat cgttgcggcg gtcatgacaa gcaggagtgg gagagaacca tgagcgaaat
     6001 ctactctgga cgctgccttt gtggcgctgt gcgcttcgag gcaaccggca agcccaagtg
     6061 ggtgcgttgg tgtcactgcg aaagctgccg gcggcatagc ggagctccag taagcgcttt
     6121 tgtttcgttc gagaatgacg ccgtcacgat aacggagggc gtcattacga aatacaactc
     6181 ttcgccgggt gttgagcgcg gtttctgcgc gcggtgcggt tcaactctca cctgtgccaa
     6241 tgttcgcctt ccgaccgaga cacactatta cgtcggggcc ttcgaacgag cagcggaact
     6301 aaaacccaca ggcgaattct tcgccggtga gcgccttccc tgggtgcata cccaggacgg
     6361 aagctcggca atagagtagg ccgtggagaa gctgcgtcat gaaaaccttc aacgatccgc
     6421 cctactgcac cgagcgcgtc tacctcgact acaacgcaag cacgcccatt gatccggcgg
     6481 ttgccgccgc gatgaagccg tttcttgcgg atcacttcgg caatccgtcc agcggccatt
     6541 gggccgccct acctgccaag accgcccttg aagaagcgcg tcggcaaatt gcggccctgc
     6601 ttggctgtga ggatgatgaa gttgtcttca caagcggtgg aagcgaggcc aacaatcttg
     6661 cgctcaaagg cgtgtacttt gcacgacgcg acagtggtga gcacatcatc accacgcgga
     6721 tcgaacatcc ggcaatcgtt gaaccatgtc gttttcttga acgcttgggc gcgcggataa
     6781 cttacctgcc agtggataga tacgggcgcg tcgatcccgg tgacttaggc caagccatca
     6841 cgccgcgcac gattctcatc agcatcatgc acgccaataa cgaggtgggc accatccagc
     6901 cgattgagga gtgcgcgcgt atcgcccgcg aacatggcgt cctctttcat accgacgccg
     6961 ctcaatcggt cggcaagatt gcaactgacg tgaacgggct cggcgtcgat ctgctttcga
     7021 tcgccggaca caagctctac gcgcccaagg gtatcggcgc cttgttcata cgtcgtggcg
     7081 tcacgctcga accgcacaat cacggggccg gacacgagag cgggcggcgc gccggaaccg
     7141 agagcgccat gcttgcggtc ggtctcggaa aggcttgcga actggcgcgc gatctagcca
     7201 caattgatcg ggtccgggtc ctgcgcgacc acttttggcg cgaactgcag gagcggttcg
     7261 acgatcgcat caccctcaac ggacatcctg cgcatcgtct gccgaacacg ctcaatgttt
     7321 ccttcattga ccacgtcggc gtcgacgtac tggaacggtt ggacggtgtc gccgcctcaa
     7381 cgggctcagc gtgccatgcc gggcgcgtcg agctatcgcc cgtgcttgag gcgatgggcg
     7441 tcgcgctgga agtgggcatg ggcgctatcc gcttcagtct tgggcgtggg acaacagacg
     7501 aggaaattga agccgtggtt gaacgcctga acgccgccat cgccgtcgcg ccatgacccg
     7561 ggatgcgtcc gggcacagca ggcatccttc agaccgtatt gatgtttcgt cgcgaagcgt
     7621 tgaatggtaa aatatcggtc tgtgatgctg ttcgcactac tagcaaaggt ttctgaatga
     7681 tgctgagcga tcaatctctt accgcaggtt catatctgtc gcagcgagcg ctgccttttg
     7741 ctgggatgaa ttggtaatgt gctcgaacct aatcaatgtt tgagatcgac gcctccttcc
     7801 gaccagccgc cgccacacta gcgctgctaa ccgcattcct gttgctgcgc gacgcaaggt
     7861 ttgaattccg ggcgcgattg ggcgcgctgt ttggaatcgg gactgccgcc tatatgtttt
     7921 gctcgggcgc tccgggggtc tggcgcgcca gctcgttgct gctgccgcta tgcattggaa
     7981 acagcgtttt cttctggtgg ttcgcgctgg cgctgctgga ggacgacttt cgtctcgaag
     8041 cagtacatgt tggcgtccta atagctttgc tggctttggg tttcgctcgt gttggtgccc
     8101 acattattga gtacggcgcg cttgggcagg cgctcgctat ctttcataac ctcgtcattc
     8161 tgacactcgt cgctcacgta ctgttgctgg cctggcgcgg ctatgacaac gacctcttgg
     8221 agaaccgcag gcggtttagg ctgctgttcc tcatcggcgg aagtctgacg gctgttggga
     8281 tagcggtcgc cgaggtcgcc cttgccgggc gcccccctgc tgcctggatg ctagcgctac
     8341 aatctgtact tgtttcgatc ctcgcgacgt cggcggcagc atggctgctc agcgcatccg
     8401 catcacgctt ggcgttccaa aacataacga ctgccaagcg ggccgaacct gctccggtta
     8461 tggatcgcca gaggctgatc gatgaggccc ttggcgtagc cttggagcgt gccatcaata
     8521 ttgacaagtt gtatcttgag cagggcttga cgatcgccgc actggccaaa agattgggag
     8581 tgccggagca tcgcctgcgc aaacacatca acgaggcgct cggccatagc aatttcaacg
     8641 cttacgtgaa ccagcatcgt atcgccgccg ccaaggccgc gttgcgagac cctaaactgg
     8701 cgcatttgcc ggtgcttacc attgcgctcg actccggctt tgcctcgctc cctcccttca
     8761 accgcgcgtt tcgcgccgaa accggccagt ccgcgacggc ttaccggagc gccgcgttgg
     8821 tggagacacg cagcgcaaat gggcggatcg aatctgaaaa aagctaggcg atttcgaaat
     8881 cgagcgcatt tggcaagaaa tcgagcggac agggtggggt tcagcggcta gttccggcga
     8941 tggttctcgt ggaggaccaa cgcaaggaga gtatccgcac catgatccaa tttacatcaa
     9001 tcctcgatga attccgccaa atctggccat atatctttgc gggcgacctc gcccgatatc
     9061 tgatcggcgc cggttccgta ttccttctca tctgggtgat tttccggtcg cgattggcag
     9121 ccagaaagat tcgaggaaat actccgccgg ccggtcaaat ctggcgggag atcggtcatt
     9181 cgctcatgac ggtttgcatc ttcgcgactg tcgggactgc gatcggaatc ggagcgatgc
     9241 atggcgtatt tcaggtctac gccgacattt ccctccatgg ctggctttat ctcgttggaa
     9301 gcgtcgttgc catgatcgtt gtgcaggata cctatttcta ctgggtgcat cggctcatgc
     9361 accgcgcacc gctgctttgg aaagtgcatg ccactcacca tcgctcgcac aatccaactc
     9421 cgtggacggc ctacgcgttc gatccgggcg aagccctgat ccacgcactg ttcatgccgt
     9481 tattcgtcgc agtcgtcccg atgcacgtta gcggcctctt cgcatttacc gcacacatga
     9541 tgctgcgcaa tgccatcggt cattgcggct acgagctgtt tccgaaaggc tgggcagatc
     9601 gtccgatact gggtctcgtg acgatggtca cccaccatga catgcatcac gagcatgcgc
     9661 cccggaattt cggcctttat tttacttggt gggaccgatt tatgggaacg gagcatccgg
     9721 aataccggac gcgcaccaag ttggtgacac cggaccatga gatgcccgac aacctcaaag
     9781 gcacctacgc gtcataaatc ggagcgagaa gctgagtcga ggaattgctt aaggtcatcg
     9841 ccctggctgg ggcgagctgg agagcgaaga ggaacaacga tgaatctcta tgagcgctgg
     9901 gtactaccgc ccattctcga tttgatcatg cgacagaagc acctcacgaa atatcgtcgc
     9961 gcggtggtcg ccgtggcccg tggccgcgtg ctcgaaattg gtgtcggctc gggattgaac
    10021 tttccgctgt atggcaaaga ggttgagctt gtctacggga tagacccctc gcgccggctg
    10081 ctctcaatcg ctcgtcggcg tgccgctgcg tcagcggtgc ccgttgaact tcatctgggt
    10141 tccgcaactg ctatcccact tgcggacgat accgttgaca ctgtggtgat gacctggaca
    10201 ctttgttcga ttcccgaccc gctggctgcg ctgcgcgaga tgcgaagggt gctcaaacca
    10261 agcggcaacc tctgctttgt cgagcacggg ctttcgcccg agccaagcgt cgagcactgg
    10321 cagcgccgga ttaccccagc ctggcgccgc gtggcgggcg gctgtcatct ggaccggaag
    10381 atagatgaac ttatccgtct ggccggcttc gatctgacga acctacaagt ggaatacgcg
    10441 ccgggacccc gcgccatgtc ctatatgtac gaaggatgcg cgtgccgcac tcattcgggc
    10501 gggatcggcc ctgccggcgc atagaccgcg ccacgcgacg accgtcctat ggttcgggtc
    10561 cgccgcaagc cagcaacatc ctggaagttg cgtctttgac tgcgccaagg ctgattggtt
    10621 ttatcccgcg tcgatcgcac tgacaaagcg ggaaaggaaa gcgcgaaagg cctccagttc
    10681 cttcttggag aaagccgcga agatcctgtc ctcgacagga gcgacttgag cgcggccggc
    10741 gtgcagcgcc tctcggccga catcggtaat gtggagttcg agcacattcg ggtgccttgg
    10801 gtgcggctgc cgcgcgatca gtccctgctg ctccatggtt ttgagcagga tcatgatggc
    10861 ctgtggactg acaaagcagg cccgcgccag ggtagcggcc gtgatgccct gtttctgttc
    10921 gagttccgcc aacgcaacgt attgcgcgag cgtgagcccc acgggcttga gcaccgcggt
    10981 cttggacttg agcatcgctt gttcggcacg cttgagaatt ttgccgaggt ttcgctcgtc
    11041 tgcccccgtt ttttttggtt gtttcatatc cagggcacga gtgtccggaa ccgcccaggc
    11101 tcgccgcgac cgaggtttat cagcccgcca tagaaggctg cgtcgggcgc cgcctccaac
    11161 gcaagatcca acaacgggat cgcaacctcg tcaggcggtg gggcgccggt catatcgagc
    11221 cattgccgcg acgcgccggt gtcgatcaat ccgggtgaga tcgcggcgat caggattccc
    11281 cgccgtaaat cctccgcccg tcgctgctgt gccagcacgc gaacggcggc tacctgaccg
    11341 atcttcgagg ggatattgat ccactccggc caggcctgtc cggccgcaag tccctgacgg
    11401 acaatatccc gccacgcgca tactgcgcgg tcaacgtcat caagcgtttg aagattctca
    11461 aagcgcccgt gcagcgcagg ggccaaggcg cgcaagctgc ccgcgcggct cgccaccacc
    11521 aacagccgcc cgttatcgcg aatgagcggc gcaaaattcc gcaggatatg ggttgtcccg
    11581 aggttgttgg cggcgacata accgtcgatg acttcagcgg gattttcgcc cggttgcacc
    11641 cgcgcgtagt ggttcgagaa cacgatatcg acgccgccat ggcggtcggc gatcgcgccg
    11701 gcaaaacgct ccacggcctc acgatccgac acgtcgagaa ct
//
LOCUS       DATNOI010000205         4176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2490717,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000205 DATNOI010000000
VERSION     DATNOI010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490717"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /gene="livM"
                     /locus_tag="VN968_07885"
     CDS             complement(<1..1134)
                     /gene="livM"
                     /locus_tag="VN968_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HXL29197.1"
                     /translation="MTASAPERTAVSRARRAVFIFKKALFSALVALVLFSLMIGVRTE
                     AGPSGQLTYWTRFDDLAAMVGAVFGGSIVVELLRQWWGPVGTIRVVPPSVQSALAVAR
                     RGIAPLLLIFTFLVPVIFYDQRYILDLGILVLTYVMLGWGLNVVVGLAGLLDLGYVAF
                     YAVGAYSYALLATNFGLSFWICLPLAGILAALWGVMLGFPVLRLRGDYLAIVTLAFGE
                     IIRLVIINWQSLTGGPNGVTGIPRPTLFGIPLTPGDDGLAARLGIEFSPTHRIVFLFY
                     LILALALLTNWVTIRLRRLPLGRAWEALREDEVACRALGINITTTKLTAFATGAMFGG
                     FAGAFFATRQGFISPESFTFQESALVLAIVVLGGMGSQLGVALA"
     gene            complement(1146..2063)
                     /locus_tag="VN968_07890"
     CDS             complement(1146..2063)
                     /locus_tag="VN968_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663086.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HXL29198.1"
                     /translation="MDYFAQQLINGIVLGSIYGLIAIGYTMVYGIVGMINFAHGDIFM
                     IGGFIALISFLILVSIGLTAVPVILLIVLLVSMAITALYGWTVERIAYRPLRHSFRLA
                     PMLSAIGMSFVLTNYSQVAQGARVKPVPPIITGGYMLHQGSGGFVVQLSNVQIIVVIT
                     TVVLLAIFTWLVARTRLGRDMRACEQDQTMAALLGVDVDRTISMTFVIGAALAAVAGM
                     MYLLYYGLVDFFMGFVAGIKAFTAAVLGGIGSLPGAMLGGLAIGLIETFWSAYFSVEY
                     KDVAAFSILIIVLIFLPTGLLGRPEVEKV"
     gene            2332..2487
                     /locus_tag="VN968_07895"
     CDS             2332..2487
                     /locus_tag="VN968_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29199.1"
                     /translation="MPNQNQNPGQQNQNPGQKPGQQQGGGQKPGQQQQDPNRQGQNPN
                     QQDKGGR"
     gene            2800..3342
                     /locus_tag="VN968_07900"
     CDS             2800..3342
                     /locus_tag="VN968_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29200.1"
                     /translation="MRTDWRISSFNGVLLAAYFIPTWAIVAFRIMISPIHGLYERPNV
                     SVALFISDHLQLAALTTVRFAWLLALGKLTVVGFFAIFVVLITRVSIRKAGGCDEALA
                     MALAIGSVISFASMVMAAQVGESQAVKLHATELLMLLGTAIVLLVEAPARRQPAQAQV
                     DRNATADALALEQAQLLNNR"
     gene            complement(3304..3696)
                     /locus_tag="VN968_07905"
     CDS             complement(3304..3696)
                     /locus_tag="VN968_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXL29201.1"
                     /translation="MDDQKRRDDGMAQRRKVLGDAWVDKSIANKNSFNTEFIDLITRH
                     AWGEIWTRPHFDQRTRRVLVIGTMVALGQWDEFRLHVRAALAEGGFTPEDIKEILLQQ
                     AIYCGVPAANHAVKEASAIVQELGLLKG"
     gene            complement(3806..>4176)
                     /locus_tag="VN968_07910"
     CDS             complement(3806..>4176)
                     /locus_tag="VN968_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL29202.1"
                     /translation="EGGIAAVADTVITGWLTADFREREPQIAANMKAMLIASPVQGYL
                     ACCEALSTLDQRALLPRIKSPTLVIAGRHDTATPIAAGEFIRSQIPGASLTILDAAHI
                     SNVEQPHAFTDAVIGFLTQR"
BASE COUNT          907 a         1303 c         1267 g          699 t
ORIGIN      
        1 cgcgagcgcg acgccgagct gcgaacccat gccgccgagc acgacgatcg ccagcaccag
       61 cgccgattcc tggaaggtga aggattctgg actgatgaaa ccctgccgtg tcgcgaagaa
      121 cgcgccagcg aaaccgccga acatggcacc ggtcgcgaat gccgtcagtt ttgtcgtggt
      181 gatgttgatg ccaagcgcgc gacaggcgac ctcgtcctcg cgcaacgcct cccaggcgcg
      241 gccgagcggc aaacgtcgca gccggatggt cacccagttg gtgagcaatg caagcgccag
      301 gatcaggtaa aacaggaaca cgatgcgatg agtgggggag aattcgatgc cgagcctggc
      361 cgcaagcccg tcatcgccgg gcgtcagcgg aatcccgaac aacgtcgggc gcggaattcc
      421 ggtgacgccg ttggggccgc cggtcaagct ttgccaattg atgatgacga gacggatgat
      481 ctcgccaaat gccagcgtga cgatggcgag atagtcgccg cgcaaccgca gcaccgggaa
      541 gcccagcatc acgccccata gcgccgcgag gatgccggcc agcggcaggc agatccagaa
      601 cgacagcccg aaatttgtcg ccagcagcgc gtaggaatag gcaccgacgg cgtaaaacgc
      661 gacatagccg aggtcgagca gcccggcgag gccgaccacc acgttcagcc cccagcccag
      721 catcacatag gtcagcacca ggatgccgag atcgagaata tagcgctgat cgtagaagat
      781 caccggcacc agaaacgtga agatcaacag cagcggcgct atcccgcgcc gcgcaaccgc
      841 aagcgcgctt tgcacggagg gcggaacgac cctgatggtg ccgaccggcc cccaccattg
      901 ccgcagcaat tcgacgacga tgctgccgcc gaacacggcc ccgaccatgg cggcgagatc
      961 gtcgaacctc gtccagtagg tcaattgtcc gctaggtccc gcctctgtgc ggacaccgat
     1021 catcagcgaa aacagcacca gcgcaaccag cgcgctgaac agggccttct tgaagatgaa
     1081 tacggcacgt cgagcgcgcg acaccgccgt acgttcgggc gcggatgctg tcacgcgctc
     1141 gcccgtcaaa ctttttcaac ttcgggccgg ccaagaagcc cggttggaag gaaaatcagc
     1201 acgataatca ggatcgagaa cgccgcgacg tccttgtatt cgaccgagaa ataggccgac
     1261 caaaacgtct cgatcaggcc gatcgcgagc ccgcccagca tcgcgccggg cagcgaaccg
     1321 atgccaccga gcaccgccgc ggtgaaggcc ttgatgccgg cgacgaagcc catgaaaaaa
     1381 tccaccaggc cataatacaa caggtacatc atgcccgcga ccgcggcgag cgctgcgccg
     1441 atcacgaacg tcatggagat ggtgcggtcc acatcgaccc cgagcagcgc cgccatggtc
     1501 tggtcctgct cgcaggcgcg catatcgcgg ccgagccggg tccgcgccac cagccaggtg
     1561 aagatcgcga gcagcactac ggtcgtgatg acgacgatga tctgcacatt ggatagctgc
     1621 accacaaagc ctccgctgcc ctgatgcagc atgtagcccc cggtgatgat cggcgggacc
     1681 ggcttgaccc gcgcgccctg tgccacttgc gaataattgg tcagcacaaa tgacatgccg
     1741 atggccgaca gcattggcgc aaggcggaac gagtgccgca gcggccgata agcgatgcgc
     1801 tccaccgtcc agccgtagag cgcggtaatc gccatcgata ccagaagcac gatcaagagg
     1861 atcacgggca cggcggtcag gccgatcgag accaggatca aaaacgagat cagcgctata
     1921 aagccgccga tcatgaaaat gtcgccatga gcaaaattga tcatgccgac aatgccatag
     1981 accatggtgt agccgatggc gatcaggcca tagatcgagc cgagcacaat gccgttgatg
     2041 agttgctggg cgaaatagtc catgcgctgc cgctacttgg atgacgcggc gatggtcccg
     2101 gcagccggaa cggacgcgtc gtcccatttt ctaacagtgg gaacccgctg cggcaacagc
     2161 gccaggtgcg gcttaagggc ttgtacatgg ggagtttttg tcccccaggt tccgccgcca
     2221 cagtcgcgac aagctgtcgt cacgcaatga tcccggcgaa acccgagttc ccctgaaacc
     2281 aattgccggc cgtgtagttg ggtcgggtta cgttcaacca gggagatccc tatgcctaac
     2341 cagaaccaga atccgggcca gcagaaccag aacccgggcc agaagcccgg ccagcagcag
     2401 ggcggtggcc agaagcccgg acagcagcag caagatccga accgtcaggg ccagaacccc
     2461 aatcagcagg acaaaggcgg acgttagttc gaacctgaac tgaaccaaac ttagcgagag
     2521 cactgaagtc ccgccgaaag gcgggacttt tctcttttgg ttatcgctca tcgtcacccc
     2581 cgggcccttg acccgggggt ccatcaacct tccaaggatt ttgcgaagaa gatggatgac
     2641 cgggtcaagc ccggccatga cggcggagcg acgagacccg gcggctccca caattaaggt
     2701 aaataaaggg tttaaattgc cgctggcagt ttaagcgagt tacctcgccc gcaacgcggc
     2761 cactccggtc cggagcagcg cggagaagcg ggaagcgaca tgcggacaga ctggcggatc
     2821 agttcattca acggcgtgtt gctggcggct tatttcattc cgacctgggc catcgtcgcc
     2881 ttcaggatca tgatttcgcc gatccacggc ctctacgaac ggccgaatgt atcagtcgct
     2941 ctattcatca gcgatcatct gcaactggcg gcgctgacca cggtccgctt cgcctggcta
     3001 ttggcattgg gcaagctcac ggtggtcgga tttttcgcga tcttcgtggt tctgatcacc
     3061 cgcgtctcga tccgcaaagc cggcggctgc gatgaagcgc tggcgatggc gcttgccatt
     3121 ggcagcgtga tcagttttgc cagtatggtg atggccgcgc aggtgggcga gagccaggcc
     3181 gtcaagctgc atgcgaccga actgttgatg ctgctcggca ctgcgatcgt attgctggtc
     3241 gaagctcccg ctcggcggca gccggctcag gcgcaagtcg atcgcaacgc gaccgccgac
     3301 gccctagccc ttgagcaggc ccaactcttg aacaatcgct gatgcctcct tgacggcgtg
     3361 gttggccgcg ggcaccccgc aatagatcgc ctgctgcagc aggatttcct tgatatcctc
     3421 aggcgtgaag ccgccttcgg cgagcgccgc gcgcacgtga aggcggaatt catcccattg
     3481 accgagcgcg accatggtgc cgatcaccag cacccgccgg gtacgctggt cgaaatgcgg
     3541 ccgggtccag atttcgcccc acgcatggcg cgtgatcagg tcgatgaact cggtgttgaa
     3601 cgaattcttg ttggcgatcg atttatcgac ccaggcatcg ccgagcacct tgcgtcgctg
     3661 cgccatgccg tcatccctgc gtttttgatc atccattgaa gtctctccta gtcattgcga
     3721 gcgcagcgaa gcaatccaga atccctccgc ggaaaacagt ctggattgct tcgtcgcttc
     3781 gctcctcgca atgacgctgt tagagttagc gttgcgtcag gaagccgatc accgcgtcgg
     3841 tgaaggcgtg cggctgctcg acattggaaa tatgcgcggc atcgaggatc gtcaggcttg
     3901 caccggggat ctggctgcga atgaattcgc cggccgcgat cggcgtcgcc gtgtcatgac
     3961 gcccggcaat caccagcgtc gggcttttga tcctgggcaa cagggctcgc tgatcgagcg
     4021 tgctcagggc ttcgcagcag gcaagatagc cctgcaccgg agacgcgatc agcatcgcct
     4081 tcatattcgc tgcgatctgc ggctcgcgct cgcggaagtc cgcggtcagc cagccggtaa
     4141 taacggtatc ggcgacggca gcgatgccgc cttcct
//
LOCUS       DATNOI010000206        16618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_674744,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000206 DATNOI010000000
VERSION     DATNOI010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 16618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16618
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674744"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VN968_07915"
     CDS             <1..1071
                     /locus_tag="VN968_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXL29203.1"
                     /translation="GSLGARNDIYASGIEHKLGMHAAPTCTMTMGDKGGAVGYLIGEE
                     NRGMLCMFTMMNQARLGVGLEGVGIADRAYQQALAFAQERRQGRAVGKQGDGSDPIIV
                     HPDVKRMLMQMRALTAAARTICYATAVALDVSVRATDAKVRDDATARGALLTPIAKAF
                     STDIGNEVASLGVQIHGGMGFIEETGAAQHLRDARITPIYEGTNGIQSIDLVTRKLAA
                     NGGASVWALLDELSGIVKRVEASNDPAFGTTGAKLRDALGSLERSSRWLLERVTSAPN
                     DALAGATPYLRLFGSTLGGCMLAGEALAARDLGEGAGDPLRYVTLARFFAENISVQAG
                     ALERTVIDSAEAVNGADAVLLG"
     gene            complement(1983..3158)
                     /locus_tag="VN968_07920"
     CDS             complement(1983..3158)
                     /locus_tag="VN968_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HXL29204.1"
                     /translation="MRVCATLPLRERDLRLDLIRGLANWAIFLDHIPHEVLSSITTRN
                     YGFSDAADLFVFISGYTAALVFGRVMIERGYLAAASRLVKRALQLYAAHIVVVVVYIA
                     VIASVSRGLHDPNDLNVFNVAVFISKPLWESIQALALRYRPVNLDVLPLYILLLATFA
                     PALWLMVRKPLLTLVGSMVVYLAGRHFGWNLPTSPSGSWYFNPFTWQFLFFLGAWIAL
                     GGAQAIQSIVRTRTVFWLAIIYLIFAFVVTMATHTPDLGNLLPPWISQPFDPNDKTNL
                     APYRIVHLVALAIAVTRFLPADSPILRWRWLTPPIKCGQNSLQVFCIGIVLSFCAHAV
                     IELSLNSFLVQIVVGATGILLMTAVAYCWTWPKQLLASSAQIRETRVTGNLASSGRR"
     gene            complement(3169..4362)
                     /locus_tag="VN968_07925"
     CDS             complement(3169..4362)
                     /locus_tag="VN968_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXL29205.1"
                     /translation="MSSEDIKPPSKRSLLTAATAAVVLGSVVCGYGFIERAESKQEVV
                     QWTNTQAIPTVALAQFIPGGRHQTLTLPGNIQPYNRAAIFARVNGYVKSWDHDIGSSV
                     KAGQVLATIDAPDLDQQLGQAKATLASVKANEQIATLTANRNNILLQKQIVAQQLADQ
                     TTADAAAKEAVVDANEANVRQLEAMQSFKTLAAPFDGVVTARNVELGMLINSGGSGQP
                     LFEVSDLHRVRIFVQVPQSFSAGLAPGMKATFEMPQYPGVQFDATLSHISKAINATSH
                     SMQVELQADNAAGKFFGGSYCNVHFELPTDANLVKLQSTALVTGNQGTQVATLDSNNK
                     VVLKSVQLGRDLGDSVEIVAGLSPSDRVINNPPETLAAGDNVRVATATPQVAAPASQS
                     KTSQH"
     gene            complement(4380..7571)
                     /locus_tag="VN968_07930"
     CDS             complement(4380..7571)
                     /locus_tag="VN968_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXL29206.1"
                     /translation="MIGIVRLALRRPYTFIVMALLILIFGTASALRTPTDIFPNINIP
                     VISVVFTFTGLPADDMAGRVVTFYERSLPNSVNDIEHIESQSIANYGIIKIFFQPTVN
                     INAALAQVNGMSQTVLKQMPPSITPPLILSFNASSVPILQLALSSDKLSETQIFDDAL
                     NFIRPALAPVPGAALPLPFGGKVRQVQADLNQQALHQYGVSANDVINALSLQNLITPV
                     GTQKIGTYEYTVNLNDSPQAVKAFNDLPVKTVNGTVVYMRDVAYVHNGSPPQINAVHV
                     NGASAVLLTIVKAGASSTLDIINGVKSLLPSVSQTLPSSLKLIAVGDQSVFVTDAVSS
                     VIKEGLIAATLTGMMILLFLGSWRSTFIITLSIPLAILAALTGLSLLGETINVMTLGG
                     LALAVGILVDDATVTMENINWHLEQGKEIEPAILDGARQIVVPATVSLLCICIAFVPM
                     FGLGGVAGYLFRPLAEAVMLALAASYVLSRTLVPTLANYLLRNQHIHGSSDGVDHAPA
                     KASRNPFLRFQRGFEHHFENVRKAYLGLMQLALQNRIKVIAGFLVFSLLSFGLAPYLG
                     QDFFPTVDGGQIKLHIRAPTGSRIEATTALADRIGTAIHGIVPANELDGIVSNIGLTV
                     SGINMAYNNSGTIGVGDADILISLKPNHAPTDDYIKTMREKLPQQFPGTSFAFLPADI
                     VSQVLNFGVPAPIDLQVAGRDLAANRKYANDLLAKVRAIPGIADARIQQAFQQPTLNV
                     NVDRSLTSLAGLTEKDVATAMLTTLSGSSQSAPTYWLDPANGVSYPVSVQTPQRDINT
                     MTGLKNIPVTSSGAGAAQLVGGLANVERTNSNAVVSHYNVQPVIDIYATPQGRDLGAL
                     ASDIQKVIHATAPDLPKGASVALRGQVSTMTSAYQQLFVGLAAAIVLIYLLIVINFQS
                     WADPFVIVMALPTALAGIVWILFGTGTTLSVPALTGAIMCMGVATANSILVISFARER
                     MAAGVNAMVAAFEAGGSRFRPVLMTALAMVIGMLPMAIEPGQNAPLGRAVIGGLIFAT
                     CATLVLVPTIFSLVHGRQHDAAKQPDARTAVSQ"
     gene            7799..8725
                     /locus_tag="VN968_07935"
     CDS             7799..8725
                     /locus_tag="VN968_07935"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL29207.1"
                     /translation="MNKTAPKLGAIDLNLLVVFDAIMRDRSVTRAGQRLGLSQPAMSH
                     ALTRLRHMLKDELFVRSPTGMVPTPRAELLATPIRIALDGLQQSLEPVQFEPAKATTT
                     FRIAVDNYAAIVLVAPVAMQVAKEAPGVTLDFRPSGTLNVPELLDRSELHLAIGPCGA
                     DGERFSVRRLLQDQFVVVHRKGHPAAKEREFSTEKLTSLPQLEISSAQFGTDFVETGP
                     GRSKSAPRPAMRAPFLSAAQILATSDLVSVLPLNVARNMTRSHQLVFRRLSRSPKPIE
                     ATMIWLRRLDNQPAHAWLRDVVTRAANDSRAA"
     gene            9569..9955
                     /locus_tag="VN968_07940"
                     /pseudo
     CDS             9569..9955
                     /locus_tag="VN968_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017964593.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(10038..10979)
                     /locus_tag="VN968_07945"
     CDS             complement(10038..10979)
                     /locus_tag="VN968_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HXL29208.1"
                     /translation="MHLTGIHHLTAISAKPRENLAFYTGLLGMRLVKKTVNQDDVSAY
                     HLFYADGKANPGTDLTFFDFPAAPERRGTNSISRTGLRVAGEKSLGFWRDRLKQAGGH
                     TGEVTEVDGRLTLPFEDGEGQRLVLVDDGGAGSASPWEKSVVPPEHQIRGLGPIVLTV
                     HDLARTEQVLTGVMNMRRVRDYAAYAAQIQVFAMGEGGLAAELHVIENADLQIARQGA
                     GGVHHVAFRTPDEAQYHAWTQRLNDLGIRNSGEIDRFYFRSLYFREPNGILFEIATDG
                     PGFATDEPMEKLGEKLALPPFLEPRRAQIEAGLKPIG"
     gene            11115..11876
                     /locus_tag="VN968_07950"
     CDS             11115..11876
                     /locus_tag="VN968_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXL29209.1"
                     /translation="MTGHLIVTDEGAARMIKLRRPEKKNAITQDMYRAMSDAIDTAQN
                     NPDIRCLIITGGSGVFTAGNDLEDFLKDGTSNADTPRASDATKFLYSLAHNVKPIIAA
                     VDGVAIGIGTTMLFHCDYVLASHTATFSTPFIHLGLVPEGASSLLMPRTMGHQRAFAM
                     LVMGRTVSAADAQVAGFVNVVVAPGHTEAEARKVAREICALPAEAVAISRKLLRLPPE
                     DLTRRIDQEGHLFGERMRSKEAVAAFKAFFSRRKA"
     gene            12160..13791
                     /locus_tag="VN968_07955"
     CDS             12160..13791
                     /locus_tag="VN968_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HXL29210.1"
                     /translation="MLNTSASRADAQTPAPPVELRILAINDFHGNLLPPTGGIRIADP
                     DDKTKKIVVPAGGAEHMATLVKQLGQGSKNTIFVAAGDLIGASPFLSAMFHDEPTIES
                     LSMMGLEVASVGNHEFDEGKDELLRMQNGGCHPVDTCLGPHPFLGARFHYLAASTVEK
                     STGKTVFPPYEIKQFDGIPVAFIGLTLKGTADIVSPVGVAGLEFKDEADTVNALVPEL
                     KAQGVEAMVVLIHEGGFPAGDYNECPGISGPIVDIVRKLDHAVDVVISGHTHQAYVCD
                     IDGRLVTNGDKFGTIVTSIDLKLDPVTRDVISAKADNVIVRTGVYAKDPEQTELLASY
                     DKFAAPVANRVAGSVTDTLSRTPNDAGESPLGDIIADAQLAATSAETNGGAVIAITNP
                     GGIRTDIPKKDAGAVSYADVFASQPFRNQLVTLTLTGMQIKNMLEQQWLDPKRPRILQ
                     VSKGFSYTWDGARAYGDRVAADRMSLNGQRIGPATSYRVTVNNYLSVGGDGFTVLKEG
                     TAPRIGVYDVDALFAFFRANSPIGPAPADRIVRMN"
     gene            13892..14821
                     /locus_tag="VN968_07960"
     CDS             13892..14821
                     /locus_tag="VN968_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HXL29211.1"
                     /translation="MTTLLLSHPASLDHLTPPGHPERPDRMRAVAEVLAEDRFGKLVR
                     GEAPEGSLDTVTLCHNEHYVEELRHIAPTSGLIYLDGDTSMSPGTWEAVMRGVGGAVA
                     ATEAVMSGAHHNAFVAIRPPGHHAEINKPMGFCFFDNAAIAARHAQRKYGIARAAVVD
                     FDVHHGNGTQDIFWADPTVMYCSTHQMPLFPGTGARGERGEHDNIVNAPLASEDGSAK
                     FRAAFENVILPQLQKFAPELIIISAGFDAHYRDPLASLNLKSDDFGWVTRKLMDVADS
                     SAGGRIVSVLEGGYDLQGLKESAAAHVTALMGG"
     gene            14892..15143
                     /locus_tag="VN968_07965"
     CDS             14892..15143
                     /locus_tag="VN968_07965"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXL29212.1"
                     /translation="MAENTQVDVKKLSFERAMEELETIVTRLEGGKVPLEESVAIYER
                     GESLKRRCEELLRQAEARVDKITTDANGQVSGAEPLDVQ"
     gene            15374..>16618
                     /locus_tag="VN968_07970"
     CDS             15374..>16618
                     /locus_tag="VN968_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29213.1"
                     /translation="MRVQHRHIIYVQGYDPRGLAQYYRMFRTELRKFGRLYALTATIS
                     RPQVAPDGETASWTIETKAGDWQTRTNYDFLRWEDLIQRDLASPIWRTVFHAMWIYWR
                     LVFGGTIARFWRAHWRFATFISYPHYLLFNEALLSLGIAFGFGKGLEALGIPGLFSLA
                     ASAAVFVAVVGTMLKYTENSTYLLYLLSDTIWTWEFSHRQRPEWDQRIDRFAQYLATV
                     ARTSGAEEIVVVGHSSGSFLGAEILARALKLDPALGRHGPRVVLLTIGGNFPIVGFHA
                     ASRDFRDHLRLLAVEPSIDWIDCQSRKDVMNFYPFDPIKGHGIDVGAARRNPTIVSVR
                     FREIIKPEHYNLFRWKFFRVHFQFVMANERPHAYDFFMIVCGPILLIERMARPEAALA
                     VATGDAAAREWAWKRLETATADP"
BASE COUNT         3266 a         5068 c         5150 g         3134 t
ORIGIN      
        1 ggctcgctcg gcgcgcgcaa cgacatttac gccagcggca tcgagcacaa gctcggcatg
       61 catgcagcgc cgacctgcac catgacgatg ggcgacaagg gcggcgccgt cggctacctg
      121 atcggcgagg aaaaccgcgg catgctctgc atgttcacga tgatgaacca ggcccggctt
      181 ggtgtcgggc tcgaaggggt cggcatcgcc gatcgtgcct atcagcaggc cttggcgttc
      241 gcccaggagc gccgccaggg ccgcgccgtc ggcaagcagg gcgatgggtc ggaccccatc
      301 atcgtgcatc ccgacgtcaa gcggatgctg atgcagatgc gcgctctgac cgcggcggcg
      361 cggacgatct gctacgccac cgcggtcgcg ctcgacgttt ccgtgcgggc cacggatgcg
      421 aaggtgcgcg atgatgccac ggcccgcggc gcgctgctga cgccgattgc aaaagcgttc
      481 tccaccgaca tcggcaatga ggtcgcctcg ctcggcgtgc agatccacgg cggcatggga
      541 tttatcgagg aaaccggcgc cgcgcagcat cttcgcgatg cgcgcattac gccaatctat
      601 gaaggcacca acggcatcca gtcgatcgat ctggtgaccc gaaaactcgc tgccaatggc
      661 ggcgcgtcgg tctgggcctt gctcgacgag ctgtccggca tcgtcaaacg ggtcgaggcc
      721 tccaacgatc ccgcgttcgg taccaccggc gccaagctgc gcgatgcgct gggatcgctc
      781 gagcgcagca gccgatggct gctggagcgc gtcacctcgg cgccgaacga cgcgctggcc
      841 ggcgcgaccc cctatctcag gctgttcgga tcgacgcttg gcggctgcat gctcgccggc
      901 gaagcgctgg ccgcccgcga ccttggcgag ggcgccggcg atcccttgcg ctatgtcacg
      961 ctggcgcggt tctttgccga aaatatctcg gtgcaggcgg gcgcgctgga gcggacggtg
     1021 atcgacagcg cggaagcggt caacggcgcg gatgcggtgt tgctggggta attcatccgc
     1081 gcacatgcgg gattcgcgcc gggcgaaaga tggattgctt cgtcgcttcg ccatgacgcc
     1141 tgctccgcca agaaccgtca tactccgcga aagcggagta tccagtacgc cgcggcttct
     1201 cgactccatc gttgacgctt cggaatactg gatcgcccgc tggagcctgt catcgggcgg
     1261 cgcgatgcgc cgacccggtg gcgggcgacg acggctgagg gtttggcgcg ctcgtattca
     1321 tcagacataa ctgcgccctc gcgacgcgta gcgtccgagg cgttgtttcg ctcgtccgcc
     1381 ccaaggaggg cgtggggaat gccggatgcc cgctgcaccc gcggtctcgt gtgcgctttg
     1441 tagtggtaag aagcacacga gtaacaacga gcacaccgga atcacccggc attcccgcac
     1501 gcaatggttt taacggctta tgtcgtgctc tccccggtga ccgggctctt ttgccaccgt
     1561 cgcttgcgga tatggttttg tctgagcccg gtagggccga cagaacatcc gcaaacttga
     1621 cgccagcgtc ggggcgtcag gaccacacga cttcgccgta cgctgcaatc gtctctcgtc
     1681 cgcgtgctgt cgatcgctca caagtctcat cgacctgccc tgcaatcccc ttgcgcgcaa
     1741 aacgctgccg cgtccaccgc atccgcaccc cgcgtccgtg acgatcgcga tacgcccctc
     1801 tggtggggtg ggatggcgcg aatttgtaga gatgatttgc ccgacgggtg aagcgaaata
     1861 tttttgcaag ggggactgga cagctagcat cagattgatc ttgttcgaca aattagcccg
     1921 agcgcgcacg ggcttcgagg atgctgcgac cgccccgacc agcgaagcca gcaaagcggc
     1981 cgtcatcttc ggccagatga tgccaaattg cccgtcacgc gagtttctcg aatttgagcc
     2041 gacgaagcga gtagctgttt cggccatgtc cagcaatatg cgactgcggt catgagcaat
     2101 attcctgtcg cgccaacgac aatctgaacc aagaacgagt tcaggctcag ttcgatgacg
     2161 gcgtgggcgc aaaacgaaag cacgatgccg atgcagaata cctgaagcga gttctgaccg
     2221 cacttgatcg gcggcgttag ccaacgccat cgaaggatgg gcgaatccgc tggcaggaac
     2281 cgcgtgactg caatggcaag agcaacgaga tgcacaattc gatagggcgc tagattggtc
     2341 ttgtcgttgg gatcgaacgg ctgcgaaatc cacggcggaa gcaaattgcc caggtcgggt
     2401 gtgtgagttg ccatcgtcac cacgaaagca aagataaggt atatgatcgc gagccagaac
     2461 accgtccggg ttcgaacgat cgattgaatg gcttgggcac cgccaagcgc gatccaggcg
     2521 cccaggaaga acaggaattg ccaggtgaag ggattgaaat accaagatcc cgatggcgag
     2581 gttggcagat tccagccgaa atgccttcca gcaagataca caaccattga accaaccaac
     2641 gtcagcagcg gtttgcgaac catcagccac agtgccggtg caaacgtcgc caacaacagg
     2701 atatagagtg gaaggacgtc cagattgacg ggtctatatc gcagtgccag ggcctggatg
     2761 gattcccaaa gcggcttgct gatgaaaacg gcgacgttga acacatttaa gtcgttgggg
     2821 tcgtgaaggc ctcgtgagac agacgcaata acggcgatgt aaacgacgac cacaacgatg
     2881 tgcgcggcat aaagttgcaa agcgcgcttt accagccttg atgcggctgc aagatagccc
     2941 cgctcgatca ttactcttcc gaacaccaag gcggcagtat aaccggagat gaaaacgaat
     3001 aggtccgcgg catcgctgaa gccgtaattc ctggtcgtga tcgagctcaa cacctcgtgc
     3061 gggatgtgat cgagaaagat cgcccaattc gccaagccgc gaatcaggtc cagtctgaga
     3121 tctcgctctc ggagaggaag tgttgcgcaa acccgcatgg aacgcgcctc aatgctgtga
     3181 cgttttcgat tgagatgcgg gtgcagcgac ctgcggcgtt gcggtggcta cgcgaacatt
     3241 gtcgcctgct gccaaggttt ccggaggatt attgataacc cgatcggacg gcgacaagcc
     3301 ggcaacgatc tccacgctgt cgccgagatc gcggccgagt tgcacactct tgaggacgac
     3361 cttgttgttg ctgtcgaggg tagcgacttg cgtgccttga ttgcctgtca caagcgcggt
     3421 cgattgaagc ttcacgaggt tagcgtcagt cggaagctcg aagtgcacat tgcagtagct
     3481 cccgccgaaa aacttgccgg ctgcattgtc agcctgaagc tcgacctgca tgctgtggga
     3541 agtcgcattg atggccttgg aaatatgaga aagcgtcgcg tcgaattgca ctcccggata
     3601 ttgcggcatt tcgaaagtcg ctttcatccc tggggcgagc ccggctgaga acgattgcgg
     3661 cacctgcacg aaaatgcgaa cccgatgcag atcggatact tcgaagagcg gttggcctga
     3721 tcctcctgag ttgatcaaca ttccgagctc cacgtttcga gcggtcacga cgccatcaaa
     3781 gggcgcggcg agcgtcttga acgattgcat cgcctcgagc tggcggacgt tggcctcgtt
     3841 ggcgtcgacc acggcttctt tcgcggcggc gtccgctgtg gtttggtccg ccagctgctg
     3901 ggcgacgatc tgtttttgca gcaagatgtt gtttcggttg gcggtcagcg tcgcaatttg
     3961 ctcattggcc ttgacgctcg ccaatgtcgc tttcgcctga cccaactgct gatcaagatc
     4021 gggagcgtcg atcgtggcca gcacttgtcc ggctttgact gacgatccaa tgtcgtggtc
     4081 ccagcttttc acgtaaccat tcacccgcgc aaagatcgct gccctgttgt agggttgaat
     4141 atttccaggc agcgtcagcg tttgatgcct gccgccgggg atgaactgag cgagggcgac
     4201 ggtcgggatg gcctgcgtat tcgtccactg cacgacttcc tgcttgctct cagcgcgctc
     4261 aatgaaacca tagccgcaga cgacgctgcc gagaacaacc gcggcagtgg cggccgtcag
     4321 gagactccgc ttgcttggag gtttgatatc ttctgaggac attttgtttt gttctccgct
     4381 cactgtgata ctgcggttcg ggcgtctggc tgctttgcag cgtcatgctg ccggccatgc
     4441 accaggctga agatggtggg caccaaaacc agtgtggcgc aggtggcaaa gatcagcccg
     4501 ccgatcacgg cgcgtccgag tggcgcgttc tgtccgggct cgattgccat cggcagcatg
     4561 ccgatgacca tggcgagggc cgtcatcagg accgggcgaa accgcgagcc gccggcctcg
     4621 aatgcagcca ccatggcatt gacgccggcc gccatccgtt cccgggcaaa gctgatcacc
     4681 aggatgctgt tggcggttgc cacacccatg cacatgatcg cgccggtgag cgcgggaacc
     4741 gaaagcgtgg tcccggtccc gaacaggatc cagacgatgc cggccaacgc cgtcggcagc
     4801 gccatgacaa tcacgaaggg atcggcccaa gattggaagt tgatgacaat caacaggtag
     4861 atcagcacga tcgccgccgc gaggccgacg aacagctgct gataggcgct tgtcatcgtg
     4921 ctgacctggc cacgtaacgc aacgctcgca ccttttggga ggtccggtgc cgtcgcgtga
     4981 ataaccttct ggatgtcgga ggcgagagcg ccgagatcgc gtccctgagg cgttgcataa
     5041 atgtcgatca ccggctgaac attgtagtgg gaaacgacgg cgttgctgtt ggtccgctcg
     5101 acattcgcca ggcctcccac gagttgtgca gcgccggcgc cggaggacgt gaccgggata
     5161 tttttcaaac cggtcatggt attgatgtcg cgctgcggcg tttgaaccga aaccgggtaa
     5221 gacacgccgt tggcgggatc gagccaatag gtcggggcgg actgcgagct tcccgacaag
     5281 gtggtcagca tcgcggtggc gacgtccttt tcggtaaggc ccgcaagcga ggtgagtgaa
     5341 cgatcgacat tgacgttaag ggtcggctgc tgaaacgcct gctggatgcg cgcatcggcg
     5401 atccccggga tcgccctgac ctttgccagc aaatcatttg catacttccg gtttgcggca
     5461 aggtccctgc cggcaacctg caggtcgatc ggcgcgggca cgccgaagtt caggacctgg
     5521 ctgacgatgt cagctggaag gaaggcgaag ctcgtgccgg ggaactgctg cggcagcttc
     5581 tcgcgcatgg tcttgatgta gtcatcggtc ggcgcatgat tgggcttgag gctgatcagg
     5641 atgtcggcat ctccaacgcc gatggtgccg gagttgttat aggccatgtt gatgccgctg
     5701 accgtcagcc cgatattgct gacgatgccg tcgagttcgt tcgccgggac gatgccgtga
     5761 atggctgttc cgatccggtc ggcgagagct gtggttgctt cgatccgtga tcccgtaggg
     5821 gcgcggatat gaagcttgat ctggccaccg tccacggtcg ggaagaaatc ctggccaagg
     5881 taaggcgcga ggccgaacga cagcaggctg aacaccagga atccggcaat caccttgatc
     5941 cggttctgca gggcgagctg catcaagccc agataggcct tgcggacgtt ttcgaaatga
     6001 tgctcgaagc cgcgctggaa gcggagaaac ggattgcggc tggctttggc cggggcgtga
     6061 tcaacaccat cggatgaacc gtggatgtgt tgattgcgca aaagatagtt cgccagcgtc
     6121 ggcaccaggg tgcgcgacaa cacataggaa gcagccagcg cgagcatcac cgcctcggcg
     6181 agcggccgaa acaggtagcc ggcgacgccg ccgagcccga acatcggcac gaaggcgatg
     6241 cagatgcaga gcagcgacac tgtcgccggc accacgatct gccgtgcgcc atcgagaatg
     6301 gcgggctcga tctccttgcc ctgctcgaga tgccagttga tgttctccat ggtgacggtc
     6361 gcgtcgtcga ccagaattcc aacggcgagc gctagcccgc cgagggtcat gacgttgatc
     6421 gtctcgccca gcagcgaaag gcccgtcagc gcggccaata tcgcaagcgg gatcgagagc
     6481 gtgatgatga acgtggagcg ccagcttccg aggaacagca ggatcatcat cccggtcagg
     6541 gttgccgcga tcaggccttc cttgatgacg ctggacactg catcggtcac aaaaaccgac
     6601 tgatcgccca ccgcgatcag cttcaggctg ctgggaagcg tctgcgacac ggaaggcagc
     6661 aggctcttga cgccgttgat gatgtcgagg gtcgaacttg cgcccgcctt cacgatggtc
     6721 agcagcacgg cgctggcgcc gttgacatga accgcgttta tctgtggcgg gctgccatta
     6781 tgcacgtagg cgacgtcgcg catgtacacg acagtgccgt tgacggtctt caccggaagg
     6841 tcgttgaagg ctttgaccgc ttgcggcgag tcgttcaggt tgacggtgta ttcgtaggtg
     6901 ccgatcttct gggttccaac cggcgtgatc aggttctgca gggagagcgc gttgatgacg
     6961 tcgttcgccg aaacgccgta ttgatgcaaa gcctgctgat tgagatcggc ctggacctgg
     7021 cgcaccttgc cgccgaacgg aagcggcagg gcggcacccg gcaccggagc caaagccggc
     7081 cggatgaaat tcagtgcgtc atcgaagatc tgggtttcgg acagcttgtc gctcgaaagc
     7141 gcgagctgca gaatgggaac gctcgaggca ttgaagctca ggatcaatgg cggggtgatg
     7201 ctcggcggca tctgcttcag cacggtctgt gacatgccgt tgacctgcgc caatgcggcg
     7261 ttgatgttca cggtcggctg aaagaagatc ttgatgatcc cgtaattggc gatcgattgg
     7321 gactcaatgt gctcgatgtc gttgacgcta ttgggcaacg agcgctcata aaacgtcacc
     7381 acacggccgg ccatgtcgtc ggcaggcagg ccggtgaagg tgaaaaccac gctgatgacg
     7441 gggatgttga tattcgggaa gatgtcagtt ggcgtccgca gggcggaagc ggtcccgaag
     7501 ataaggatca gcagggccat cacgatgaag gtatacggcc gtcgcagcgc aagccgaacg
     7561 attccaatca tagcgtcctc ctgtgcgaga ccatgctccg gtcgccttaa ccgccgatcg
     7621 ttagagcgga cttagcgtcg cttccgcccg accgtggtcc aatgccgctc gcgcatccgt
     7681 gaacgcgcgc tgctgcaaca gacaagctgg aatctagggt tgagtttcgc attcggaaaa
     7741 tgcattctct tcatccgcac atgcttgatt tgaatgaccg ttaaggagtt gcggtcgcgt
     7801 gaacaagaca gcccccaaat taggcgcgat cgacctgaac ctgctggtgg tgttcgacgc
     7861 catcatgagg gaccgatcgg tcacgcgggc ggggcagcga ttaggcctca gtcaacctgc
     7921 catgagccat gcgctgaccc gtttgcgcca catgctgaaa gacgaactgt tcgtgcggag
     7981 cccaaccggg atggtgccga cgccgcgggc cgaactgctc gccacgccga tccggattgc
     8041 gctcgacggg ctgcaacagt cgctggaacc cgttcagttc gagccggcaa aggcgacgac
     8101 gaccttccgc attgccgtgg acaattatgc cgcaatcgtt ctggtcgcac cggtagcgat
     8161 gcaggtcgca aaggaggctc ccggggtgac gctggatttt cggccgagcg gcacgttgaa
     8221 cgttcccgaa ctgcttgatc gaagcgaact gcatttggcg atcggtccgt gcggcgccga
     8281 cggcgagcga ttttcggtca ggcggctgct gcaggaccaa ttcgtcgttg tgcaccgcaa
     8341 gggtcatccg gcagcgaaag aacgggaatt ctcgacggag aagttgacga gcctgcccca
     8401 actggaaatc tcgtccgccc aattcggcac cgattttgtc gagacgggcc ctggacgatc
     8461 aaagtctgcc ccaagaccag cgatgcgtgc gccattcttg tcggcggcgc agatcctggc
     8521 gacgtccgac ctggtatcgg tgcttccgct caatgtcgcc cggaacatga caagatcgca
     8581 tcagcttgtg ttccgtcgac tgtcgcgttc gcccaagccg atcgaagcta ccatgatttg
     8641 gctgcgacgc ctcgacaacc agcctgcgca tgcctggctg cgcgacgtcg tcacccgtgc
     8701 agccaatgac tcccgtgccg cgtgaacttg caaaaatccg atggaaccaa atcaacaaac
     8761 ctgcacggat cgaacggcca aaatcgagaa acaccctgca tgacgcacca tcgggaactt
     8821 tggcgccgcc ttcgaagttc atcccgtgga taagttcttc ccgtggatat gacaactcgg
     8881 caagggacca ccaactatga ggcgagcgca gcctgtttca atcttcgctc gcggatggat
     8941 gatcggcaga gtggccgtca tcagcgtggc ggtgctgggg ctgctggcct ctcccgcgcc
     9001 ggcccaggat gccggcgtca gcggcattcc tccggggcct gcaaatgcgc gcggattaaa
     9061 cggctcggtc agcgatccca gcggcatcgg caacgcggcc aaagtgcccg cgataccgcc
     9121 gccagtcatt acccccgtgc ccgtacccac tctatccggt cctgctggta cttaccgaac
     9181 atcccctact taccgaacgt tcccggtgca gcgatcggtc aggatgagac gcacgcggtc
     9241 tgcggcatcc ggatctcgtc gctcggctgc ccgagcggcg gtcagggaga aagacaggct
     9301 gctcgaccac aagattacaa gcatctgcag gggatgctga tcgacatacg cgacgcgtgg
     9361 cgcgtccccc tggtccggat ccgtcggctt ccgagtgacc ggggacatcg ataacagttc
     9421 agaccgacga cacctggagc tgttcgcggg cgtagcgagg ctacaccgcc cggactgtcg
     9481 ccagaaaact ctcgacctct tgcttcaggc gctcggactg ctttgcgagt tcgccggccg
     9541 aatcgagaac cagttcggcg gcagttctgg ctcgccccag tatttcgagc gacggccgaa
     9601 gccgggaaag ccgcaagacc tcaccgccca cacctgcatc aatatacgct tgccgaccta
     9661 cggcggaatc tacgcctggg aattcgagaa acgcgggcgt ggcttgcggg ttcgggtgga
     9721 agggcagctg gtgttcaacc acgtcgccct gaggctgaat gcggtactgg ccgggtttgg
     9781 cctcgcgtac ctgccggagg accaggtgaa gacccagctc gccgacgggt ggctgatccg
     9841 ggtgcttgca gactggtgcg cgccttttcc gggctaccac ctctactacc cgagccgccg
     9901 acagccggcg ccggccttcg ccttgctggt tgacgcgctg cgctatcggg gctgacctgc
     9961 gcgctgattc attaagtcgt cattccgggg cgcgctcttg tgcgagcccg gaatgacgac
    10021 ctttgtttgt gaccattcta cccgatcggc ttcaatcctg cttcgatctg cgcgcgccgc
    10081 ggttccagga acggcggcag cgccagtttt tcgccgagct tttccatcgg ctcgtcggtg
    10141 gcaaagccgg gtccgtcggt ggcgatctcg aacaggatgc cgttgggctc gcggaaatac
    10201 aggctgcgaa aataaaaccg gtcgatctcg ccggagttgc ggatgccgag gtcattcagc
    10261 cgctgcgtcc acgcatgata ttgcgcctcg tccggggtgc gaaacgcgac gtggtgcacg
    10321 ccgccggcgc cctgccgcgc gatctgcaaa tccgcattct cgatcacgtg cagctccgcg
    10381 gccagaccgc cctcgcccat cgcaaacacc tggatctgcg cggcgtacgc ggcatagtcg
    10441 cgcacgcggc gcatgttcat cacgccggtc aggacctgct cggtgcgggc gagatcgtgc
    10501 acggtgagca cgatcggacc aaggccccgg atctggtgtt cgggcggcac gacgctcttc
    10561 tcccacggcg aggccgaacc ggcgccgccg tcatcgacca gcaccagccg ctggccctcg
    10621 ccatcttcaa acggcagcgt cagccggccg tcgacttcgg tgacctcgcc ggtgtggccg
    10681 ccggcctgct tcaggcgatc tcgccagaaa ccgagacttt tctcgccggc gacgcgcagt
    10741 ccggtgcggg agatgctgtt ggttccacgg cgctcgggtg ccgccggaaa atcgaagaaa
    10801 gtgaggtcgg tgccgggatt ggccttcccg tccgcataga acagatgata ggcgctgacg
    10861 tcgtcctggt tgacggtttt cttgaccagc cgcatgccga gcaggccggt gtaaaacgcc
    10921 agattctcgc gcggtttcgc ggaaatcgcg gtcagatggt ggatcccggt caagtgcatc
    10981 gcatgatcct catcctttgg acaacttgcc cgggaaaatc ggcggaggca tttttgcctc
    11041 atcagggatc atgtaggata tggtccggcg actcgccagc ggtttgcatt gccgcttgtt
    11101 cggaggacag cttcatgacc gggcacctga tagtcaccga cgaaggcgcg gcgcgcatga
    11161 tcaagctgcg acggccggaa aagaagaacg cgatcacgca ggacatgtat cgcgccatga
    11221 gcgacgcgat cgacaccgcg cagaacaatc cggatatccg ctgcctcatc atcaccggcg
    11281 gctccggggt atttaccgcc ggcaacgatc tggaggattt tctgaaggac ggcacctcga
    11341 acgccgacac gccgcgcgca tccgacgcca ccaagttcct ttattcgctc gcgcacaacg
    11401 tcaagccgat catcgccgcg gtcgatggtg ttgcgatcgg catcggcacc accatgctgt
    11461 tccattgcga ttacgttctc gccagccata ccgcgacgtt ctcgaccccg ttcatccatc
    11521 tcggtctggt tccggagggc gcctccagcc tgttgatgcc gcgtaccatg gggcatcagc
    11581 gtgcgtttgc gatgctggtc atggggcgca ccgtcagcgc agccgatgcg caagtcgccg
    11641 gcttcgtcaa tgtggtggtg gcgcccggcc acaccgaggc cgaggcgcgc aaggtggcgc
    11701 gcgagatctg cgccctgccg gccgaagcgg tcgcgatctc gcgaaagctt ctaagactgc
    11761 cgcccgagga tctgacccgc cggatcgatc aggagggcca tttattcggc gaacggatgc
    11821 gctccaagga agcggtggcc gcattcaagg catttttctc gcgcaggaaa gcttgagatt
    11881 tcttaacccc gcacttgggg gcgaggcact gcgccaaccg aatcaaaatc tctcgggtca
    11941 tatcaatatc gaagttgaat gatatatttt cgccatcgaa tgaaacggcg gaaaattccg
    12001 ctgcgtgagg cgcgaatgtg cgacccgaga ggctcgttga tcggcaagcg cagtgcctgg
    12061 cccctcaccc ggcgcgtcgc gccgacctct ccccgtaaag aacggggaga ggttaagaag
    12121 gccgcatccg ctgctctcgc aattctcacc actgcactga tgttgaacac ttcggcctcg
    12181 cgcgcggacg cacagacacc cgcgccgccg gtcgagctac gaattctcgc catcaacgac
    12241 tttcacggca acctgctgcc gccaaccggc gggatcagga tcgccgatcc cgacgacaag
    12301 accaaaaaga tcgtcgtccc agccggcggc gccgagcaca tggcgacgct ggtcaagcaa
    12361 ctcggccaag gcagcaaaaa caccatcttt gtcgcggccg gagacctgat cggcgccagt
    12421 ccgttcctgt cggcgatgtt ccacgacgag cccaccatcg aatccctgtc gatgatgggg
    12481 ctggaggtcg cctcggtcgg caatcacgaa ttcgacgagg gcaaggatga actgcttcgg
    12541 atgcaaaacg gcggctgcca tcccgtcgac acatgtctgg ggccgcaccc gttcctcggc
    12601 gccaggtttc actacctcgc ggccagcacg gtcgagaaga gcaccggcaa gaccgtgttc
    12661 ccgccctacg agatcaagca attcgacggc attccggttg ccttcatcgg cctgaccttg
    12721 aagggaacgg ctgacatcgt ctcaccggtg ggcgttgcag gcctcgaatt caaggacgag
    12781 gccgacacgg tgaatgcgct ggtgccggaa ctgaaggcac agggcgtcga ggccatggtc
    12841 gtgctgatcc atgaaggcgg ctttccggcc ggcgattata atgaatgccc gggcatatcc
    12901 ggcccgatcg tcgacatcgt ccggaaactc gaccacgccg tcgacgtggt gatcagcggc
    12961 cacacccatc aggcctatgt ctgcgacatc gacggccggc ttgtcaccaa tggtgacaaa
    13021 ttcggcacta tcgtcacatc aatcgatctg aagctcgatc ccgtgacccg cgacgtcatc
    13081 agcgccaagg ccgacaacgt catcgtccgc accggcgtct atgccaaaga tccggaacag
    13141 accgaactgc tggcgtcgta cgataaattt gccgcgccgg tcgccaaccg cgtggcgggg
    13201 tcggtcactg acacgctgtc gcgcacgccc aacgatgccg gcgagagccc gctcggcgat
    13261 atcatcgccg atgcccagct ggccgcgacc agcgccgaga caaatggcgg cgccgtgatc
    13321 gccatcacca atccgggcgg tatccgcacc gacattccga aaaaagacgc gggtgcggtg
    13381 agctacgccg atgtattcgc cagccagccg ttccggaacc agctggtgac gctgacgctg
    13441 accggaatgc agatcaagaa tatgctcgag cagcaatggc tcgatccgaa acggccgcgg
    13501 attctgcagg tctcgaaggg cttcagctac acgtgggacg gcgcccgggc gtatggcgac
    13561 cgcgtggccg ccgatcgcat gtcactcaac gggcaacgca tcggacccgc gacgagctat
    13621 cgcgtgaccg tgaacaatta tctatcggtc gggggcgacg gctttaccgt actgaaggaa
    13681 ggcaccgcgc cgcgtattgg cgtctacgat gtcgatgctc tcttcgcctt cttccgggca
    13741 aacagcccga tcgggcccgc gccggccgat cgcatcgtgc gaatgaactg aattgcaaag
    13801 cccgatcggc gggctcggct cttcccgagc ccgcgtcgac cccctagatt agctggtcct
    13861 cgcaagcgcg ttcgacgcgc caaggtattg catgacgacg cttcttctct ctcatccggc
    13921 aagcctcgac cacttgaccc cgccgggaca tccggaacga cccgatcgga tgcgcgcggt
    13981 cgccgaagtg ctcgcggagg accgcttcgg caagctcgtg cgcggcgaag caccggaagg
    14041 cagtctggat acggtcacgc tctgccacaa cgagcattac gtcgaggaac tccggcacat
    14101 cgccccaacg agcggcctga tctatctcga cggcgacacg tcgatgtctc ccggaacctg
    14161 ggaagcggtg atgcgcggcg tcggcggcgc ggtcgccgcc accgaggcgg tgatgtcagg
    14221 cgcccaccat aatgcgttcg tcgcgatccg cccgccgggc catcacgccg agatcaacaa
    14281 gccgatgggc ttttgtttct tcgacaacgc cgccatcgcc gcgcgccatg cccagcgcaa
    14341 atacggcata gcgcgggccg ccgtggtcga ttttgacgtg catcatggca acggcaccca
    14401 ggatattttc tgggccgatc cgacggtgat gtattgctcg acgcaccaga tgccgctgtt
    14461 tcccggaacc ggcgcccgcg gcgagcgcgg cgaacacgac aacatcgtca acgcgccgct
    14521 ggcctccgaa gatggcagcg ccaaattccg cgccgccttc gaaaacgtaa tcctgccgca
    14581 attgcagaaa tttgcgcctg agctgatcat catctccgcc ggcttcgacg cgcactaccg
    14641 cgatccgctg gcgtcgctca atctcaaatc ggatgatttc ggctgggtca cgcgcaagct
    14701 gatggacgtc gccgattcga gcgccggcgg ccgaattgtc tccgtgcttg agggcggcta
    14761 tgacttgcaa ggcctcaagg agtcggctgc ggcacacgtc acggcattga tgggcgggtg
    14821 aatgcccgcc acaatacctc cgcaaaattg tctctgaatc ggcgccggcg ggcgcatccc
    14881 gggaaagcga tatggctgaa aacacccaag tggacgtcaa gaagctgagc ttcgaacgcg
    14941 cgatggaaga gcttgaaacc atcgtcacgc ggctcgaagg cggcaaggtt ccgctggagg
    15001 aatccgtcgc catctacgag cgtggtgagt ccctgaaacg gcggtgcgag gaattgctgc
    15061 ggcaggcgga ggcgcgggtc gacaagatca ccaccgacgc caatggccag gtgtccggtg
    15121 cggagccgct cgacgttcaa tgacgccgcg accatttggg tgccagtgcg ccgcccccgc
    15181 taacattaga gaatatttga cgaaattagc cgaattcgac gccaagcgac ccgcacgagg
    15241 gctgaaatag ctcttggcgc gttacgcggc ctgttatagt ccccgcggtc acagccgatt
    15301 tgaagtgcct gatcttgcgg caactggtgc aggaatttcg aatcggcggc actcgtccgg
    15361 ggatcagcaa taagtgcgcg ttcagcaccg ccatatcatc tatgtgcagg gatacgatcc
    15421 gcgcggcctt gcgcagtatt accgcatgtt tcgcaccgaa ctgcggaagt ttggccggct
    15481 gtatgcgctc accgccacca tcagccgtcc gcaagtggcg cccgatggcg agaccgcatc
    15541 ctggaccatc gagaccaagg caggcgactg gcagacccgc accaattacg attttctgcg
    15601 atgggaagac ctgatccagc gcgaccttgc ctcgccgatc tggcgcacgg ttttccatgc
    15661 gatgtggatc tattggcgcc tggtgttcgg cggcacgatt gcgcggttct ggagagcgca
    15721 ttggcggttc gcgaccttca tcagttaccc gcattacctg ctgttcaatg aagccctgct
    15781 gtcgctgggc attgcgttcg gcttcgggaa aggtctggaa gcgctcggca ttcccggcct
    15841 gttcagcttg gccgcgtcgg cggcggtgtt cgtggccgtg gtcggcacca tgctgaaata
    15901 cacggaaaac tcgacctatc tgctctatct gctgtccgac acgatctgga cctgggaatt
    15961 ctcacaccgc cagcggccgg aatgggacca gcgcattgac cggtttgcac aatatctcgc
    16021 caccgtcgca cgcacgagcg gcgccgagga aatcgttgtc gtcggccaca gttcgggatc
    16081 gttcttgggc gccgagattc tggcgcgcgc gctcaagctc gatccggctc tcggtcgaca
    16141 cggcccgcgc gtcgttttgc tcacgatcgg cggcaatttc ccgatcgtcg gctttcatgc
    16201 cgcgtcaagg gatttccggg atcacctccg cctgctggcg gttgagcctt cgatcgactg
    16261 gatcgactgt cagtcgcgca aggatgtgat gaatttctac ccgttcgatc cgataaaagg
    16321 ccacggcatc gatgtcggcg ccgccagacg caaccccacc atcgtatcgg tccgattccg
    16381 cgaaatcatc aagcccgagc actacaacct gttccgctgg aaattctttc gagtccattt
    16441 ccagttcgtc atggccaacg aacggcccca cgcctacgat tttttcatga tcgtctgcgg
    16501 gccgatcctg ctgatcgaac gcatggctcg tcccgaggcg gcgctggcag tggcgaccgg
    16561 cgacgccgcc gcccgcgaat gggcctggaa aaggctggaa accgcgaccg ccgatccg
//
LOCUS       DATNOI010000207         7135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2166985,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000207 DATNOI010000000
VERSION     DATNOI010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2166985"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..552
                     /locus_tag="VN968_07975"
                     /pseudo
     CDS             226..552
                     /locus_tag="VN968_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(571..954)
                     /locus_tag="VN968_07980"
     CDS             complement(571..954)
                     /locus_tag="VN968_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL29214.1"
                     /translation="MPMVTWDHIHLRSPDPEATAAWLEDILGGVIIRGPGRIDVKLGG
                     ANVFIAPVTAGDGVNTSPVTPYQGLDHFGLAVKDIDAVAAEIKAKGVEFTKEPTTIRP
                     GVRICFIRGPQGISVELLERDRKYA"
     gene            1254..1910
                     /locus_tag="VN968_07985"
     CDS             1254..1910
                     /locus_tag="VN968_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29215.1"
                     /translation="MNTGLHPIEWTELALARLARVGGDEFSDQPEGVEARTFRRGLIW
                     LPAALIIGLGAWMLMFGSSSKTTAESDPVRPAAAAAPEPVPSPTANIRQEGSPPNPAT
                     APVETTAPLPLEAIAPVAAAPVDGLKISSQYWRRGGLGSKALVTFTLRNGNDYAVKDI
                     EISCAFARRDGSHLTDRRRTIHDTLNMKSRKTFGHLHVGFVNVNADRAKCSPVAARRV
                     "
     gene            2049..2480
                     /locus_tag="VN968_07990"
     CDS             2049..2480
                     /locus_tag="VN968_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29216.1"
                     /translation="MPVVRYFLFVGGVLLALLLLSNVVLPQLPVEAAKVEAGPNISVI
                     RIYSDRKWPERVVFDTNLPTIVPQQTATILVRAPAQTTVADVSAKSRALQSFAEFKPS
                     EPKKPEPKPVHKRRIARAHVVPPMELVAQQPRFGFFANNTW"
     gene            2626..2790
                     /locus_tag="VN968_07995"
                     /pseudo
     CDS             2626..2790
                     /locus_tag="VN968_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024030.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
     gene            2832..3278
                     /locus_tag="VN968_08000"
     CDS             2832..3278
                     /locus_tag="VN968_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HXL29217.1"
                     /translation="MTKMKIGKGDWIVVCDGRKALILENLGDRVFPNLHTKEVHEHPD
                     MSTSAQGSDAPGRVHQSMGSARSSIEQTDWHDESERTFLKTLAERLNVAVTTGETTAL
                     TMVASPRALGMIRSDYSAAVRKALHGELDKDLVRMPVHEIEKQLLA"
     gene            complement(3338..5284)
                     /locus_tag="VN968_08005"
     CDS             complement(3338..5284)
                     /locus_tag="VN968_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXL29218.1"
                     /translation="MTFLTTSPPLARALAERNYDSPTPVQTAVSADAAADRDLLVSAQ
                     TGSGKTVAYGLAIAKKLLDGAERFERAAAPLALIVAPTRELALQVQRELAWLYQYADA
                     RVVSCVGGMDPRREQRELAAGAHIVVGTPGRLCDHLRRGRLDVSELKAVVLDEADEML
                     DLGFRDDMEFILKTTPDTRRTLLFSATLPRGIVALAKQYQQQAFRVEVAGDEGGHADI
                     EYRAIRIAAGDVEHAVVNVLRFFESPSTLVFCNTRHAVRHLQATLLERGFSVVALSGE
                     LTQNERTQALQALRDGRARVCVATDVAARGIDLPKLDLVIHADLPNDAEVMQHRSGRT
                     GRAGRKGVSVLLVPAARKRRAELLLNLAGIDAVWSTAPTADEIRKLDQERMLRDALFA
                     AETTSDDLILARALLAERSPEDIATALARLYRARLPSPEDILDPGQGAGRSRDISGRE
                     KDGWASRGDDRATAPRAKTAKASPPHGMADGSVWFRAAIGRKKNAEARWLLPMICRRG
                     GIDKHDIGAIRILETTTEFEISKAVAEAFAVKIRRPDKEDNIRIEPLADAAQSLAPSE
                     KRVHAPHEAKQAKQADRSSRQNDRSRDGPKHNGKFHNAGSSRFDKEAGSRTKQWNQDR
                     QPQLAGASPAKPAFGNKAKKHRRG"
     gene            6242..6832
                     /locus_tag="VN968_08010"
     CDS             6242..6832
                     /locus_tag="VN968_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HXL29219.1"
                     /translation="MVGLLYALPGTQLTRRLAEEGRLHKGHDLMKVEQAGDQCTLGCN
                     FDTKRPLRDILVDYVFSPTAYAGRLSRLVVNLDRSDRRRDLPDGDMRKRLGGIDSVHK
                     IMRALPEVREPFWKTFVEVAKTNPGALRYIVMLMALYMHLGPFSKRVISEIDRRVAEL
                     DGMHPVRAPGSSVRARPVKATGNNNRKNGQVSAVNS"
BASE COUNT         1369 a         2147 c         2226 g         1393 t
ORIGIN      
        1 aactggcccg cgaaatgcat ggacgtcact gcactggccg ggtgctggag caggagacac
       61 gtgagcgact gggtcgattc cggcggcgat gcgccgtatt tctgtaccga cgacggggaa
      121 ctccaggatc tggaaaacca agatctggaa aacaaagacc cggatctttc gctaccccgc
      181 cgcgcggggc gccggaaagt gccgctgacg gtcggcctcg cggcgacgct gctggcgctg
      241 gcgtcgcatc tcggacatgc gcgtgaccgc ggccaatatg ccaacgccaa ccccgaactg
      301 aaagcgtggt tcgaaggcct gaagagcggc aaggggccct gttgctccga tgccgacggc
      361 accgcggtca gcgacgtcga ctggcaatcc cacggcgggc actatcgcgt gtggctcgac
      421 ggcgaatgga tcgatgttcc cgacgaagcc gtgatcaccg aacctaaccg catcggccga
      481 accatggtct ggccgttgcg cggctatctc gggctgacca tccgctgctt catgccgggc
      541 agcatgacgt agctccgatc gcgccggccc tcacgcatat ttccggtcgc gctccagtag
      601 ctcgaccgag atgccctgcg gtccgcggat gaagcagata cggacgcccg gacggatcgt
      661 cgtcggctcc ttggtgaatt cgacgccctt ggccttgatc tcggcggcca ccgcatcgat
      721 gtccttcacc gccaacccga aatggtcgag tccctgatag ggcgtcaccg gcgacgtatt
      781 gactccgtcg cccgccgtga ccggcgcgat gaacacattg gcgccgccga gcttgacgtc
      841 gatccgcccg ggtccgcgga tgatcacgcc gccgaggatg tcctcaagcc aggccgcggt
      901 cgcctccgga tccggactgc gcaaatggat gtgatcccag gtgaccatcg gcatcgttgc
      961 tctcccttcg tttttgttgc aggtcgcttg tggccgcgct ggcgccaatt ttagcggtcg
     1021 cgttcgcgca tggccatccc ctcgatcgcc gacgggccga tctcgtcatt gcattccagg
     1081 attatcgggc ttgaacgcaa ccattttcgt tttcgtcgat tatggataag gtaaagcggc
     1141 ggaaccgcga aatcggcagt cgtgtggaag gccttgcggt gtattgcccg cgcggccttt
     1201 ttacgatgcg gcgcaatagg caggatccgc ttcgcgtgtc gacggcatga ccaatgaaca
     1261 ccgggctgca tccgatcgaa tggacagagc tagcgctcgc aaggctagcc agggtcggcg
     1321 gcgacgaatt ttcagatcag cctgaggggg tggaggccag aaccttcagg cggggattga
     1381 tctggttgcc ggccgccctg atcatcgggc tcggggcttg gatgctgatg ttcggctcct
     1441 ccagcaaaac aaccgccgaa agtgatccgg tgcgcccggc ggcggccgcg gcaccggaac
     1501 cggtgccatc gcccaccgcg aacatccgcc aagagggatc cccgcccaat ccggcgacag
     1561 cgcccgtgga aacgacagcg ccactgccct tggaagcgat cgcacctgtg gcagcggctc
     1621 ccgtcgatgg gctgaagatt tcatcgcaat actggcgccg gggtgggctg ggatcgaaag
     1681 ccctggtcac attcacgctg cgcaacggca acgactatgc cgtcaaggat atcgagattt
     1741 cctgcgcctt tgcccgccgg gacggaagcc atttgaccga ccgcaggcgg acgattcatg
     1801 acaccctcaa tatgaagagc cgcaaaacct ttggccacct gcatgtcggc tttgtcaacg
     1861 ttaacgccga ccgagccaag tgctcgccgg ttgccgcccg ccgggtgtag ctgcggctat
     1921 cggccgggag cggtaagcac acgcggtaac atcgccgtta tctgggcacc gcagccctag
     1981 gaaccaatcg cgccatcccc agttaagcaa tggtccgcgc gcccgcgcgg atgggagcga
     2041 aacctaaaat gcccgtggtg cgatattttt tgttcgttgg cggagttctg ctcgcgctgc
     2101 tgcttctcag caatgtagtt ctgccgcaat tgccggttga ggcggcgaag gtggaggcgg
     2161 gccccaacat atccgtgatc aggatctact cggatcgaaa atggcctgaa cgggtggttt
     2221 tcgacaccaa cctcccgacg atcgttcccc aacagacggc aacgatactg gtcagggctc
     2281 cagcccagac cacggtcgcc gacgtctcgg ccaagtcgcg cgcgctgcag tcatttgccg
     2341 agttcaagcc gtccgaaccg aagaagccgg aaccgaagcc ggtgcacaag cgcagaattg
     2401 caagagccca cgtggtcccg ccgatggaac tggtcgccca gcagccgcga tttggttttt
     2461 ttgccaacaa tacctggtag gccagcttgc tgggcgcctg aaggcgatgc tgtgaagtag
     2521 gttgcgagcg aagctcagcc gccgcggcag atactcttga tttgagtcgt tcagcagccc
     2581 gtcacgcgca tctgatgcag ccttgatgta ggcgtcggcc gcggtgacgg cgatctcgac
     2641 aactggcgcg atctccgcgc cgaggcgtcg cgggcccaca acaaccgtac ggcatcttac
     2701 gtgatcggga cgccgatgct ggcggactgt ctggaggggg ctctatcgca gttcggcaag
     2761 tcccttgagg atttcgaaga gcatctttga tcccatctca aacgacaacc aggtttcccc
     2821 ggagcaccgc aatgaccaaa atgaagattg gcaagggcga ctggatcgtc gtttgcgacg
     2881 gtcgaaaggc gctgatcctc gagaatctcg gcgatcgcgt gtttccgaac ctgcacacaa
     2941 aggaagtgca cgagcacccc gacatgtcga cgagcgcgca gggaagcgat gcgccgggca
     3001 gggtgcatca atcgatgggg agcgcgcgaa gctcgatcga gcagaccgac tggcatgacg
     3061 agtcggaacg gaccttcctg aagacccttg ccgagcggct caacgttgcg gtcacaacgg
     3121 gcgaaaccac cgcgctgaca atggtcgcgt caccgcgtgc gctgggcatg atccggtcgg
     3181 attattccgc cgcggttcga aaagcgctcc atggcgaact ggacaaggat ctcgtcagga
     3241 tgccggtcca cgaaatcgag aaacaactat tggcgtgaat gtcggaattc cggggcatac
     3301 cgggcctttc atccgttgac cagcaggcgc gcgcgcttca gccgcgacga tgcttcttgg
     3361 ccttgtttcc gaatgcaggt ttcgccggcg atgcaccggc aagctgcggt tgcctatcct
     3421 gattccattg tttcgtcctg gaacccgctt ccttgtcaaa tctcgaactg cctgcgttgt
     3481 gaaatttgcc gttgtgcttc ggcccatctc gagatctatc gttctgacga gagctccggt
     3541 cggcctgctt ggcctgcttg gcttcgtgag gcgcgtgcac ccgtttttcc gaaggcgcca
     3601 gactctgggc agcatctgcc agcggctcga tgcggatgtt gtcttccttg tcggggcgcc
     3661 ggattttgac ggcgaacgcc tctgcgaccg ctttggaaat ttcgaactcg gtggttgttt
     3721 ccagaatgcg gatggcgccg atgtcatgct tgtcgatacc gccacgacgg caaatcatcg
     3781 gcagcagcca gcgcgcctcc gcgtttttct tccgtccgat cgcggcgcga aaccatacgc
     3841 tgccgtccgc catgccgtga ggcggcgatg cttttgccgt cttcgctcgt ggcgcggtcg
     3901 cgcgatcgtc gccccgcgac gcccagccgt ctttttcccg accgctgatg tcacgggatc
     3961 ggccagcgcc ctggccgggg tcgagaatgt cctcgggcga gggaagccgc gcccgataca
     4021 gccgcgccag cgccgtggca atatcctcgg gcgaccgttc ggcgagcaac gcccgcgcca
     4081 gaatcaggtc gtctgacgtc gtctccgccg cgaacagcgc atcgcgcagc atgcgctcct
     4141 gatcgagctt gcgaatttca tccgccgtcg gagctgtgct ccagacggcg tcgatgcctg
     4201 ccagattaag cagcaattcc gcgcgccgct ttcgcgcggc gggcaccagc aggacgctga
     4261 cgcccttgcg gcccgcacgg ccggtgcggc cagagcgatg ctgcatgacc tccgcgtcat
     4321 tcggcagatc tgcgtgaatg acgaggtcaa gcttcggcag atcgatgccg cgcgccgcca
     4381 cgtcggtggc cacgcagacg cgagcccgtc catcccgtaa ggcttgcagc gcctgggttc
     4441 gctcgttctg ggtcaattcg cccgatagcg cgaccacgga gaagccgcgc tccagaagag
     4501 tcgcctgcaa atgccggaca gcgtgtctgg tgttgcagaa caccagcgtg ctcggtgatt
     4561 caaaaaatcg caacacattg acgaccgcgt gctcgacatc gccggcggcg atacggatgg
     4621 cccgatactc gatatcggcg tggccacctt cgtcgcccgc gacctcgacg cgaaaggcct
     4681 gttgttgata ttgctttgcc agtgcgacga tgccgcgcgg caacgtcgcc gaaaacagta
     4741 acgtgcggcg ggtgtccggc gtggtcttga ggataaattc catatcgtcg cgaaatccga
     4801 gatcgagcat ctcgtcggcc tcgtcgagca cgacagcctt cagttccgag acgtcgagac
     4861 ggccgcgccg caggtgatcg catagccgcc caggcgtgcc gaccacgatg tgggcgcctg
     4921 cggcaagctc gcgctgttcg cggcgcggat ccatgccgcc gacgcaggaa acgacgcgcg
     4981 catcggcata ttgatagagc cacgccagtt cgcgctgcac ctgcaaggca agttcccgcg
     5041 ttggcgcgac gatcaaggcg aggggtgctg cagcccgctc gaaccgctcg gcaccatcaa
     5101 gaagcttttt tgcgatggcc agcccatagg cgacggtttt gcccgagccg gtttgcgccg
     5161 aaaccagcaa gtcgcggtcg gcagcggcgt ccgccgacac ggcggtctgg acaggcgtcg
     5221 gagaatcgta gttgcgctcc gccaaggctc gggcgagcgg cggactggtg gtcaagaatg
     5281 tcacgagatg cagaccttgg ctggcgtttg gcgccggaac attgaacagg aacgggtcga
     5341 agctgctgcc tggaggatgc gcagccgcat ggcccgacgg atttgagtga ggtggggatg
     5401 cttttaggtc aagcggagcc aaaacgccat tgattgctgg cggattcctg caaggtcggc
     5461 tggcgcgcct ttggtagtgg gtcagttctg ggatgatcag gaaaaatacc tagagtgcgt
     5521 agagctacgt gctttcaaat ccggctgctg cccgtagaac cgacgcgatt gtccaaacgt
     5581 aaacagcggg atagccggat tctcggattg attgttttta aattgctagt tcaagtagct
     5641 ggttggttcc taccgagcaa aggacactcc atgacgctcc atcagctaat tccaaccagg
     5701 gcgcctatca ctgatcgatc cggcgtactc cggttgaagg cgcttttctg tgaggagcgt
     5761 tgccgcgaat cctccgatcc gacatcaaaa caagactggg catagttggc gatcgagtgg
     5821 cacacgatgg cgcatcttgc ggcgaggctg aatggtgatt cggaggaggg cagttgaacc
     5881 gcaggggcgt tgaattcacc gtcacgcaag tggagccggg attgtggaaa tggcatttcc
     5941 aaatcggcga aacaattacg actggcaaga cgcactcgaa cctcatgttc cgattcgctg
     6001 gtcagtattg atctgtttcc gactgattgg atggaacgtg ctcaacaaag cacactctag
     6061 gagcctgtcc gagtaatcgg atttttctcg acgaagaaca agggtcgtga ttcaaacggt
     6121 ggatgagcaa gacatttcgt ccttgggacg ttgatcaggt ttggctgctg ccgccgtcga
     6181 ttcaggattt ggtgccttcc gggcacatga cttcatcgag gagacgaaca ttccggtctg
     6241 catggttgga ctactctatg cgctgcctgg cacacagctg acgcggcggc tagccgagga
     6301 aggccgcctg cacaaaggcc atgacctcat gaaggtcgag caggcgggcg accaatgcac
     6361 gctcggctgc aatttcgata ccaagcgtcc gcttcgtgac atccttgtgg attacgtgtt
     6421 cagcccgacg gcatatgccg gacggctgtc aaggctcgtt gtcaatctcg accgctccga
     6481 ccgccgccgc gacctgccgg atggcgacat gcgcaagagg cttggcggca tcgacagcgt
     6541 gcacaagatc atgcgggcct tgccggaggt tcgcgagccg ttctggaaga ccttcgtcga
     6601 ggtcgccaag accaatccag gcgcgctgcg ttacatcgtg atgctgatgg cgctgtacat
     6661 gcacctcggg ccgttctcga agcgcgtgat cagtgagatt gaccgccgcg ttgccgagct
     6721 ggacggtatg catccggtga gggccccggg gtcgtctgtt cgtgctcgtc cggtaaaggc
     6781 tacgggcaat aataaccgga agaatgggca ggtttcagcc gtgaactcat aaaaatgcag
     6841 ttggatggac ggcaaggtcc gctatgccac cgatagctgc ctggcagact cacctgcggg
     6901 cgggaaggat caactccttg ggaatgtcgc actgtctcat gggggctcat ggggactgtt
     6961 ccactactct atcgggtgat tcagacggtc tcccgactgg caaaaacgcc caaaacacga
     7021 tcgttggtca gtagcgccga gagagtggca cccgctatcc cactgtctcg tatctcattc
     7081 cggtgtttcg ctatctccca gggtgactta ctgctttccc ggggctgggg gtttc
//
LOCUS       DATNOI010000208         9849 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2542489,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000208 DATNOI010000000
VERSION     DATNOI010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9849
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2542489"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VN968_08015"
     CDS             <1..182
                     /locus_tag="VN968_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29220.1"
                     /translation="AFVRAGAGDLAVRHVAAAGIFVFSILERDDFSSDRHPALSFCLS
                     MIFSENRYPLFRIML"
     gene            498..1844
                     /locus_tag="VN968_08020"
     CDS             498..1844
                     /locus_tag="VN968_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXL29221.1"
                     /translation="MKDLTQGSIVSHISTMAPQIFAGMIMIMLCQLIDLYFVSDLGDA
                     AIAGVGAAGNAGFLINALMQVLSVGTVALISHAAGRKDQADANLVFNQSLGLSAACGL
                     FTLIAGSALARSYMRSIAADHVVVEAGTTYLLWFMPALALQFAILVMASALRGTGIVR
                     PTMIVQTSTVILNIILAPVLIAGWGTGWALGVAGAGLASSIAIAAGGVMMWIYFHRYE
                     HYVVINRKLCRPQIAQWKRILNIGLPAGGELAILFVSMAVIYYALSDFGAAAQAGFGI
                     GTRLLGVVHMPALSIAFAAGAIAGQNFGAGNGERVKETFRSVLLLGTAVMLAFTVLAQ
                     WRPELWLGAFTKDPETIAVGALFLRMISLNLVAQGLIFVCSSMFQGLGNTKPALLSSG
                     ARLIMYGLPLIWLSAKPGFRIEQVWYLSIATTTLQAALSLWLLRLEFRKRLVPVAP"
     gene            2179..3981
                     /locus_tag="VN968_08025"
     CDS             2179..3981
                     /locus_tag="VN968_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL29222.1"
                     /translation="MTAEVDKRPAAIRVVIPFVFRHWLNQPATAATVAGGLLGATVAD
                     LFMPLFSGHLVDAVTLGASDPAARRAAFTAFGAIVALGLVSMTLRLVGLQAIVPFTLR
                     IMSDVSRDAFMRVQRFSTDWHANSFAGSTVRKITRGMWALDLLNDTILMALLPSLAVL
                     LGSMILLGLHWSSLGLVIAAGALIYVSMTVVFSTRYIAPAARISNAWDTKVGGTLADA
                     LTCNAVVKSFGAEAREDARLARVISRWRTRVRRTWLRYNYTSTAQLVVLLCVRGSVVG
                     GALLLWIAGRASPGDVTYVLTSYYIIHAYLRDVGMHINNLQRSVNDMEELVAIHDEAI
                     GIADAAGALPISVHGGRIVFEDVTFHYGGHRIPLYDGLSVDIRAGERVGLVGRSGSGK
                     TTFVKLVQRLYDVSGGRILIDGQDIAKATQHSLRSQIAIVQQDPILFHRSLAENIAYG
                     RPGASMAAIEQAARLANAHEFILRLPKGYGTLVGERGVKLSGGERQRVALARAFLADA
                     PVLILDEATSSLDSESEALIQQAMERLMKGRTSIVIAHRLSTVRSLDRILVFDRGEIV
                     EQGTHAVLATRTGGIYRGLFERQATEFDRVSAAE"
     gene            4264..5373
                     /locus_tag="VN968_08030"
     CDS             4264..5373
                     /locus_tag="VN968_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL29223.1"
                     /translation="MHGTRKLPDLIGEIYDAALEPALWNDVVVSINDFIGSRGCGLVS
                     KDLTSHCGRTLYYHGVDPHYIKLYAESYSRFDPLVSLPAFGQVVGIPDLIDYDEYRKG
                     PFYQEWLRPQRCVDVANALLEKPASNSAILLAVLPGARMLDDEMRRRIAQIVPHAHRA
                     LLINRTIDSRQSEAATFADTLDGLSAGIFLVDAGCRIVHANVAGDEMLRADDFLCSIG
                     GQLVAGNAQVNRTLREIFADRRGAPGGKGTALPLTGHDGERYVMHVLPLTSAARTAIG
                     VAYKAVAALFVRKVALGTPSGELLARNFELTPAELRVLLSIVEIGGVPETAATLGVAE
                     TTVKTHLHRVFAKTGTRRQADLVKLAAGFSSPLAV"
     gene            complement(5393..6301)
                     /locus_tag="VN968_08035"
     CDS             complement(5393..6301)
                     /locus_tag="VN968_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXL29224.1"
                     /translation="MPSPKLSPEHLGLLLGFIGMAIFGGTLPATRLAVSGFDPLALTA
                     LRTAIAGLCALTLLLVLRRPLPPRRLWFQLAIAMLCVSILFPFLMALAVQVVDASHGG
                     VVLGILPIATALVAVAITHERPKPLFWVASVAGASLVIAFALRQGGGALSAGDLQLFA
                     AVAIAAIGFTFSGRLTAEMPGWEVISWVQVIALPISLPAAALTLPADLTHIALDPWLA
                     LLYVAVFSQWIGFFAWNAGMAMGGITRVSQVQLLQPFITFALAALFNDETITLQILMF
                     AAAVVATVAIGTGTRVRETPVVQGSP"
     gene            complement(6340..7074)
                     /locus_tag="VN968_08040"
     CDS             complement(6340..7074)
                     /locus_tag="VN968_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXL29225.1"
                     /translation="MTFALLLLGALAGGFVSGLAGFGTALMALGIWLYVVPPSIAVPL
                     VLICSILGQTSTMPSFWRSIDFKLVWPFLIGGLAGVPLGTLLIAHADPRIFKVSVGVL
                     MLVFPTALYFNRTPIALSFGGRLADATIGFAGGILGGLAGLSGPLPILWASVRGWGKD
                     ERRGVFQTFSWTVLSAALCLQAGTGFITGQVLWLALLVLPATLFGAWLGARVYRVLSD
                     RNFHDVVLGLLFLSGVGLVWSGLGLR"
     gene            7331..8050
                     /locus_tag="VN968_08045"
     CDS             7331..8050
                     /locus_tag="VN968_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL29226.1"
                     /translation="MGATFLVCHGAWSAGWAWKKMHPLMAAAGHRLVTPTYTGLGERA
                     HLANPSIDLETHIRDILNVIKYEDLRDIILIGHSYGGMVATGVADRARDRISQLIYLD
                     AFVPKDGQSLFDLNEPGRQWMLGLAKAGDGWRVPPNPTPPDTPPTDVEWLTGRRVPMP
                     IKCFEMKLALHGGEPTLPRSYVYATRIAPADTFGPFAARAKSEPGWRYYEIDASHAPN
                     VTAPEALMALLDKIRYDESGE"
     gene            8377..8631
                     /locus_tag="VN968_08050"
     CDS             8377..8631
                     /locus_tag="VN968_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HXL29227.1"
                     /translation="MRKYIIPIVAAVGLGMAAPAAAYDSAGLISMQAALDIATDLGLV
                     TVSRTQFAGDEWQVEGRDRSGRYMEVDVDATTGEVLNVDR"
     gene            complement(8866..9283)
                     /gene="rnpB"
                     /locus_tag="VN968_08055"
     ncRNA           complement(8866..9283)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VN968_08055"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            9559..>9849
                     /locus_tag="VN968_08060"
     CDS             9559..>9849
                     /locus_tag="VN968_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXL29228.1"
                     /translation="MQYLLMIYQNEAEYGKIDAATGKKMMEEYGAFTQSIVQSGNFKA
                     GDRLQDTHTATTVRVREGKTLTTDGPFAETREQLGGYYLVEAKDLDAALAIAA"
BASE COUNT         1804 a         3062 c         3108 g         1875 t
ORIGIN      
        1 gcgcttttgt ccgcgctggc gctggcgatc tcgctgttcg gcatgtggca gcggctggaa
       61 tttttgtatt ttcaattcta gagcgggatg acttttcttc ggatcgtcat cccgctctat
      121 ctttttgttt gagcatgatc ttttcggaaa accggtaccc acttttccgg atcatgctct
      181 gagcgcgtcg ctcgccatat tgcaaattgc ccgcgacgca tctataagca cggcgccgtt
      241 gctcgaaccg gggagggatt tgcatgattt cgatgtattc gaagatcgcg ccctcgcgtc
      301 caatgctcat gccggacgcc cgtctggccc tctgagggcc acctccgtca gcatcgatcg
      361 ggcctgacgt cccgatcttc caaaatttcc cgctaagtca tcgccatttt ttcaacgacg
      421 tccgtcgttc ggccctgtgc cggcgcgcgt cacgcaacgg agccggtcgg cgcgcaagcg
      481 cgattggatg tgctggtatg aaagatctca cgcaaggctc gatcgtcagc catatctcca
      541 ccatggcgcc gcagatcttt gccggcatga tcatgatcat gctctgccaa ctgattgatt
      601 tgtattttgt ttctgatctg ggcgacgcgg cgatcgccgg cgtcggcgcg gcgggcaatg
      661 ccggctttct gatcaacgcg ctgatgcagg tgctgagcgt aggcacggtt gcgttgatct
      721 cgcatgccgc ggggcgcaag gatcaagccg atgccaatct ggtgttcaat cagtcgctcg
      781 gtttgtcggc ggcttgcggg ttgttcacgc tgattgccgg atccgcgctc gcacgcagtt
      841 acatgcgctc gattgcggcg gaccatgtcg tcgtcgaggc gggtacaacg tatctgcttt
      901 ggttcatgcc ggcattggcg ctgcagttcg ccatcctggt catggcctcg gccttgcgcg
      961 gcaccgggat cgtgcggccc accatgatcg tccagaccag cacggtgatc ctcaacatca
     1021 ttcttgctcc agttctgatc gcgggctggg gcaccggctg ggcgttgggc gttgccggcg
     1081 ccgggcttgc gagctcgatt gcgattgcag ccggcggggt gatgatgtgg atctattttc
     1141 accgctatga acactacgtc gtcatcaatc ggaaactgtg ccgtccgcaa attgcgcaat
     1201 ggaagcgcat attgaacatc gggctgcctg ccggaggcga actcgctatt ctgttcgtct
     1261 cgatggccgt gatatattat gcgctcagcg atttcggcgc ggcggctcag gccggcttcg
     1321 gcattggcac gcgcctgctg ggcgtggttc atatgccggc gttgtccatt gcgttcgcgg
     1381 ccggggccat cgccgggcaa aacttcggtg ctggcaacgg cgagcgggtg aaggaaacct
     1441 tcagaagcgt cctgcttctc ggcaccgccg tgatgctggc tttcacggtg ctggcacagt
     1501 ggaggccgga gttgtggctc ggagccttta ccaaggaccc cgaaacaatc gccgtcggcg
     1561 cgctgttcct gcggatgatc tctctgaacc tagtcgcgca agggttgatc tttgtgtgtt
     1621 caagcatgtt tcagggattg ggtaacacca aaccggcgct gttgagctcc ggtgcgcgcc
     1681 tgatcatgta cgggctgccg ttgatctggc tttcggccaa gcctggtttt cgcatcgagc
     1741 aggtctggta tttgtcgatt gcgacgacca cgctgcaagc ggcgttgagt ttatggttgc
     1801 tgcgcttgga gttcaggaaa cggctggtgc cggtcgcgcc atgaggcagg gcaccgcagt
     1861 ttccttgcac gactttggcg ccggcatgtt gcaaaacgcc cgtgatgcat ttataagcag
     1921 tggattgtcg ctcgaaccgg ggaggatttg catgatttcg atgtattcgc agatcgcgcc
     1981 ctcgcgtccg aagccagtat cggatgcccg cgtggccctt tgagggccgt ttccgccagc
     2041 atcggtcggg cctgacgtcc cgacctccct tgctcccgta agtcaccgcg attttttcaa
     2101 cgacgcttgc gttcggccat gccgtcgctc gtcaaccaac ggagccggtc cgcgcgcgag
     2161 cgtagccgga tgacgccgat gaccgcagaa gtcgacaagc gccccgctgc gatccgcgtg
     2221 gtgatcccgt tcgtgtttcg ccactggctc aatcagccgg ctactgccgc gaccgttgcc
     2281 ggcggcctgc ttggcgcgac cgtcgccgat ctgttcatgc cgctgttttc cggacatctg
     2341 gtcgatgcgg tgacgctggg cgcatccgat ccggccgcgc ggcgcgcggc gttcaccgcg
     2401 ttcggcgcca tcgtggcgct gggcctcgtc tcgatgacgc tgcgccttgt cgggctgcag
     2461 gcgatcgtgc cgttcacgct gcggatcatg tccgacgtgt cgcgcgacgc cttcatgcgc
     2521 gtgcagcgct tctcgaccga ctggcacgcc aacagcttcg ccggctctac cgtgcgcaag
     2581 atcacccgcg gcatgtgggc gctcgatctg ctcaacgaca ccattctgat ggcgctgttg
     2641 ccatcgctgg cggtgctgct gggctcgatg atcctgctcg gcctgcactg gtcttcgctc
     2701 ggcctcgtga tcgcggcggg cgcgctgatc tacgtgtcga tgacggtggt gttctcgacg
     2761 cgctatatcg cgccggcggc gcgcatctcc aacgcctggg acaccaaggt cggcggcacg
     2821 ctggccgatg ccttgacctg caacgcggtg gtgaaatcgt tcggcgccga agcgcgcgag
     2881 gacgcgcggc tggcgcgcgt catcagccga tggcgcacgc gggtgcgccg gacctggctg
     2941 cgctacaact acaccagtac agcgcagctc gtggtgctgc tctgcgtccg cggctccgtg
     3001 gtcggcggcg cgttgttgct gtggatcgcg ggtcgcgcct cgccgggcga cgtcacctat
     3061 gtgctgacca gctactacat catccacgcc tacctgcgcg atgtcggcat gcacatcaac
     3121 aatttgcagc gttcggtcaa cgacatggag gaactggttg cgatccacga cgaggcgatc
     3181 ggcattgccg atgccgccgg cgcgttgccg atcagcgtcc acggcggacg cattgtgttc
     3241 gaggatgtca cgttccatta cggcggccat cgcatccccc tctacgatgg cctgtcggtg
     3301 gacatccgcg ccggcgaacg cgttggcctg gtcggccgct ccggctccgg caagaccacc
     3361 ttcgtcaagc tggtgcagcg gctttacgat gtcagtggcg gacgaatcct gatcgacggt
     3421 caggacattg ccaaggcgac gcagcattcg ttgcgcagcc agatcgccat cgtgcagcag
     3481 gatccgatcc tgtttcatag atcattggcc gagaacatcg cctacggccg gcccggcgcc
     3541 agcatggcgg cgatcgagca ggccgcgcgg ctggccaatg cgcatgagtt catcctgcgg
     3601 ctgccgaaag gctacggcac gctggtcggc gagcgcggcg tgaaactgtc gggcggcgag
     3661 cgccagcgcg tggcgctggc gcgggcgttt ctcgccgacg cgccggtgct gatccttgac
     3721 gaggcgacct cgagcctgga ttccgagtcg gaagccttga tccagcaggc gatggaacgc
     3781 ctgatgaagg gccgcacctc gatcgtgatc gcgcatcgtc tgtcgacggt gcgcagcctc
     3841 gaccggatcc tggtgttcga tcgcggcgag atcgtcgagc agggcaccca tgccgtgctc
     3901 gccacccgga ccgggggaat ctatcgcggg ctgttcgagc ggcaggcgac ggagttcgat
     3961 cgcgtgtcgg cagcggagta gcgcggcagg tcatcggagc gcatccggcg ggttcgcgcc
     4021 aacggctcgc gcgaatgcgc gcccgatggc gggctccgcg cggtccgatg acaggttccg
     4081 acgcgaaccg ggaatagcga cctcattttg gggttcgtca aacatgcttg gcggatagcg
     4141 gtgaacctca aaggccgccg acagacccat ctacggcggc ggtttaatta tcttatcctc
     4201 cgatcggagg ataaggccgc tcagtgagac gctacttggc cagctacgcg acggatcagc
     4261 cctatgcacg gaacccgaaa acttcccgat ctcatcgggg aaatctatga cgcggcgctc
     4321 gaacccgcat tgtggaatga cgtcgtcgtc agcatcaacg atttcatcgg aagccggggc
     4381 tgcggacttg tctcgaagga tctgacgagt cactgtgggc gtacgcttta ttaccacggc
     4441 gtcgatcccc actatatcaa gctttacgcg gaatcttatt ccagattcga tcctctcgtc
     4501 agcttgccgg ccttcgggca agtcgtgggc attccggacc tcatcgacta tgacgaatac
     4561 cgcaaggggc ccttctatca ggagtggctg cgaccccagc gttgtgtcga tgttgcaaac
     4621 gcgttgctgg aaaaaccggc ttcgaattct gcgattctcc ttgcggttct cccgggcgcg
     4681 cggatgctcg acgatgaaat gcggcggcgc atcgcgcaga tcgtgccgca tgcccaccgg
     4741 gcgctgctca tcaacaggac gattgattcc aggcaatccg aagccgcgac atttgccgac
     4801 acgctggacg gtctcagcgc cggaatcttc cttgtcgatg ccggctgccg gatagtccat
     4861 gcaaacgtag ccggcgacga aatgctgcgc gcggacgatt ttctgtgctc gatcggcggg
     4921 cagctcgtcg ccggcaacgc gcaggtcaat cggactttgc gcgaaatatt cgccgatcgt
     4981 cgcggcgcgc cgggcggcaa gggcacagcc ttgcctctga cggggcatga cggcgagcgt
     5041 tacgtcatgc acgtgctacc gctgacgtcg gcggcgcgga ccgccattgg cgtggcctac
     5101 aaggccgtcg ccgccttatt tgtccgaaag gtggcgctgg gcactcccag tggcgaactt
     5161 ctcgcgcgca atttcgaact gacgccggcg gagctgcgtg tgctgctttc catcgtcgag
     5221 atcggcggcg tgccggagac ggcggcaact ttgggtgtcg ccgaaaccac cgtcaaaacc
     5281 catttgcacc gggtgttcgc caagacagga acccgccgcc aggccgatct cgtcaagctc
     5341 gccgccggat tttccagtcc gttggcggtc tgatgcatga gcccgacggc ggtcaaggcg
     5401 aaccctgaac gaccggcgtt tcccgaaccc gcgtgcctgt gccgattgcc acggtggcga
     5461 cgacggccgc ggcgaacatc aggatttgca gcgtaatcgt ctcgtcgttg aacaatgccg
     5521 cgagcgcaaa agtgatgaag ggctgcagca gttgcacttg cgatacgcgc gtgatgccgc
     5581 ccatcgccat gccggcattc cacgcgaaaa acccgatcca ctgcgagaac accgcgacgt
     5641 agagcagcgc gagccacgga tccaaggcga tgtgcgtcag atccgccggc agggtcagcg
     5701 cagccgcggg cagtgagatc ggcagcgcga tcacctgcac ccagctgatg acctcccatc
     5761 ccggcatctc ggcggtgagc cgtccagaga aggtaaaacc aatcgccgcg atggcgaccg
     5821 cggcgaacag ctgcagatcg ccggcggaga gcgcgccgcc gccctgccgc agcgcgaacg
     5881 cgatcaccag cgacgcgccg gcgacggacg cgacccagaa cagcggtttt ggccgctcat
     5941 gggtaatggc gacggcgacc agcgcggtgg cgatcgggag aattcccagc accacgccgc
     6001 catgcgaagc atcgacgacc tgcaccgcca gcgccatcag gaacggaaac aggatgctca
     6061 cgcacagcat cgcgatcgcg agctggaacc acaatcggcg cggcggcagc ggccgtcgca
     6121 gcaccagcag caaggtcaac gcgcacagcc cggcgatcgc ggtgcgaagc gccgtgagcg
     6181 caagcggatc gaatcccgaa accgccaggc gcgtcgcggg cagcgtaccg ccgaagatgg
     6241 ccatgccgat gaaacccagc aatagaccca gatgttcggg agacaatttg ggggaaggca
     6301 tggcatctga ggtagcgggt tcgctcgcac catgcaaggc taccgcaatc cgaggccact
     6361 ccagacgaga ccgacgcctg acagaaacaa caggccaagc acgacgtcgt ggaaattccg
     6421 gtcgctcaac acccgataaa cccgcgcgcc gagccaggcg ccgaacaacg tcgccggcag
     6481 gacgagcagt gcgagccaaa gcacttgccc ggtgatgaat ccggtccccg cctgcaggca
     6541 cagcgcggct gacagcacgg tccagctgaa ggtctgaaag acgccgcgcc gctcgtcctt
     6601 gccccagccc cgcacgctgg cccacaatat cggaagcggg ccggatagac cggcaaggcc
     6661 gcccagaatc ccgcccgcga atccgatggt ggcgtctgcc aatcggccgc cgaagctcaa
     6721 cgctatcggg gtgcggttga aatagagcgc cgtcggaaac accagcatca gcacgccgac
     6781 gctcactttg aagatgcggg ggtcggcatg agcgatcaac agggtaccca gcggcacgcc
     6841 ggcgaggccg ccgatcagga atggccagac cagtttgaag tcgatcgacc gccaaaacga
     6901 cggcatcgtc gaagtctgcc cgaggatgga acagatcagg accagcggca ccgcgatcga
     6961 aggcggcacc acgtagagcc agattccgag cgccatcagc gcggtcccga agccggcaag
     7021 gccggacacg aagccgccgg cgagcgcgcc gagcaataga agcgcaaatg tcatggcttt
     7081 cggattgatc ctgtcaggcc gtcattgcgg ggcgcatcga agatgcgaac ctcagatgtg
     7141 caattgcaca tccgggaatc cttggattcc gggttcgtgg cggagcctgt catcgggccg
     7201 cgctttgcgc ggacccgttg gcacgccccg gaatgacggc ctttgaattg gcgggcgtcg
     7261 tttacatcgt atgacaggcc ggtagtatta caaattgacg ctggtccaag aactcaacaa
     7321 ggaaaccatc atgggagcaa cctttctcgt ctgccacggt gcatggtcgg caggatgggc
     7381 gtggaagaag atgcatccgc tgatggccgc agcggggcat cgcctggtca caccgaccta
     7441 caccggcctt ggcgagcggg cgcatctcgc gaacccgtcg attgatctcg aaacccacat
     7501 ccgcgatatc ctcaacgtca tcaaatatga agacctgcgc gacatcatcc tgatcggcca
     7561 cagctatggc ggcatggttg ccaccggcgt cgccgaccgc gcgcgcgacc gtatttcgca
     7621 gttgatctac ctcgatgcgt ttgtcccgaa ggacgggcaa tcattgtttg atttgaacga
     7681 gccggggcgg cagtggatgc tgggactggc gaaggccggc gacggctggc gggtgccgcc
     7741 caatccgacg ccgcccgata cgccgccaac cgatgtcgag tggctgaccg ggcgccgcgt
     7801 ccctatgccg atcaaatgtt ttgagatgaa gcttgcgctg cacggcggcg agccgacgct
     7861 gccgcgcagc tatgtctacg ccacccgcat cgcacccgcg gacaccttcg gtccatttgc
     7921 cgcgcgcgcc aagagcgagc ccggctggcg ctattacgaa atcgatgcca gccacgcgcc
     7981 gaacgtcacc gcgccggaag cgctgatggc gctgctggac aagattcgat atgacgagag
     8041 tggcgaatag ggagtagcga atagcgaatg gtaagccgct ccctatcgat actttttcga
     8101 ttcgctactc gccattcgcc attcgccttt ttgaaagaat ccacccagcg gggagtttcg
     8161 ccccgcaatg acggttgcag aattggcgag gggtccggcg tgctgcgccg aaaaaaccca
     8221 tctccttatc gccaacatcc ggtcaacaaa tggccgcggc cgcgcgcggt ttcgtgatgt
     8281 gcaaaatcgg aacccacggc gcttcgaacg gttttcgggg aggttatgcc ggtcggaagg
     8341 cgtaacggga aagatagctt cgacgaggag taacccatgc gaaaatatat cattccaatc
     8401 gttgcagccg tagggctcgg catggcagcg cccgccgcgg cctatgactc ggccggcctc
     8461 atttcgatgc aggcagcatt ggacattgcg accgaccttg gtcttgtcac cgtatcccgc
     8521 actcaattcg ccggtgacga atggcaagtc gaggggcggg acaggtcggg ccgatatatg
     8581 gaggtcgatg tcgacgcgac caccggtgaa gtgcttaatg tcgaccggta gtcgcgccgg
     8641 gtgcgtctcg aacggatgat gcaaaaggtg ggatgggcgt aagtaattcg ccgtcattcc
     8701 gaggcgtgct aaggggtctg cgcaaaggcg cggtcccgat gacaggcgcc gctacggacc
     8761 ccgaatgtcc ggattccggg ttcacgcgaa gacgggtgcc ccggcatgac agtcgttgat
     8821 atggcgaggg gtccggcgtg ttgcgccgaa cccctcattt tcaaagatat cagccagcct
     8881 gtaagccggg ttttgtaggg caccgcctgc ttgcgcaaac gatacgtgac ggccattcct
     8941 ctgggacaac gtttgcacgt tgcctcgagc aacctacccg gacggcgagc ctgacactgc
     9001 cctgcggcgt tttcgctttc gcgaacgagc ccgcgttgcc gtccctattc ggtcttgctc
     9061 ccggtgtggt ttgccatgcc gtttccgttg ccggacccgc ggtgcgctct taccgcacct
     9121 tttcaccctt gcctcctccg aagaggaggc ggttcgttct ctgtggcact gtccctgggg
     9181 tcgcccccgc cggacgttat ccggcaccgt atgtcgatgg agcccggact ttcctctccc
     9241 gcagcctttc ggccgtagca ggagcggccg cccggccgac tgacgcgcta ggcatggggg
     9301 ctccgggccg ccgcgtcaag ccgataacgg cacggggccg gatcccatcg cgccatcaag
     9361 acgaaaaagg ccgttaccgc cagctacatc agagccttgc gccttgcgaa atgcctaaca
     9421 ggctgcccgc gatcaggcgg ttcgcctcga ttgtgtccac cgcatcggtc aaaataaatt
     9481 atcctgaaga tgtcgttttg cgagtggccc gttcgactgg atgtcggcga atggcgccgg
     9541 tcaagaggag tgagcaaaat gcagtatttg ttgatgatct atcagaacga agccgagtac
     9601 ggcaaaatcg acgctgcaac gggcaagaaa atgatggagg aatacggtgc attcacccag
     9661 tccatcgtcc agagcggcaa tttcaaggcg ggcgaccggc tgcaggacac gcacacggcg
     9721 accaccgtcc gcgtccgcga gggcaagacc ctcaccaccg acggtccgtt cgcggagacg
     9781 cgcgagcaac tcggcggcta ctatctggtc gaagccaagg atctcgacgc cgcgctcgcc
     9841 atcgccgcg
//
LOCUS       DATNOI010000209         5040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_236605,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000209 DATNOI010000000
VERSION     DATNOI010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236605"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VN968_08065"
     CDS             <1..237
                     /locus_tag="VN968_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HXL29229.1"
                     /translation="ALWLYRKVVFGALVKPSLASIKDLTFRESLTLMPLVFLTILFGI
                     YPKPVLDMSAASVQQLVTNYSHAVTAMKAAALLQ"
     gene            361..1800
                     /gene="nuoN"
                     /locus_tag="VN968_08070"
     CDS             361..1800
                     /gene="nuoN"
                     /locus_tag="VN968_08070"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HXL29230.1"
                     /translation="MSFESAGYQLLPVLPELALAAGAMVLLMLGAYRGGPQTTSLVTG
                     LAVCLLVLVGVLELLLPAGKLTTFSGSFIVDDFARFLKILTLTGSAATLILSAEFLAD
                     PSRRIFEYAILVLLSTLGMMVLISAGDLIMLYLGLELMSLALYVVAASNRDNAKSTEA
                     GLKYFVLGALSSGMLLYGASLVYGFTGTVSFAGIAAAAKTGSVGIVFGLVFLLAGLCF
                     KVSAVPFHMWTPDVYEGAPTPVTAFFASAPKVAALAVFTRVALTAFPDIVSQWQQIVV
                     FVAIASMALGSFAAIGQKNIKRLMAYSSIGHMGFALVGLAAGTVEGAQGVLVYISIYV
                     AMTLGTFAVILTMKRNGQHVETISDFAGLSRTNPLLAFFFAMLLFSLAGIPPLAGFFA
                     KWYVFVAAIKAGLFTLAVVGVLTSVVGAFYYLTIIKVMYFDEPLGQLDPMRMELRTVL
                     AVAGLFNIFFFVYPGPLVSMATVAAKSLF"
     gene            1801..2613
                     /locus_tag="VN968_08075"
     CDS             1801..2613
                     /locus_tag="VN968_08075"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HXL29231.1"
                     /translation="MTFSLGPRAASAGYRLAAFDSIGSTNAEAIARARGGEPGPMWFV
                     TSEQTAGRGRRHRPWIAPRGNLASSILEVIDVSPAVAATLGFAAGLALEAALQRVSVE
                     ASLRAAGSDHMKFSLKWPNDVLAGRQKLAGILLEAEAVAGDRLAVVVGIGTNVIAAPE
                     ATPTPATSLAALGVNIGAEELFAVLSDSWTEFRGIWDRGRGFGEIRRLWLERAAGLGH
                     GVAIQSGSAIVEGTFDTIDEQGCMIVRTVDGKRIPIAAGDVYFGSVASVGAT"
     gene            2613..4283
                     /locus_tag="VN968_08080"
     CDS             2613..4283
                     /locus_tag="VN968_08080"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HXL29232.1"
                     /translation="MARPDELTFAPLGGVGEIGMNLSIYGLGNRHQRSWLAVDLGVSF
                     GDEEHLPGIDLIMPDIRFLEKERKNLIGLVLTHAHEDHFGAIMDLWPKLKCPIYATKF
                     SAALFEAKCASERNPPKIPVTVVPSGGRIDLGPFSVEFIPVAHSIPESHALAIHTSAG
                     TVLHTGDWKIDPTPIIGVPTDERRLREIGDAGVLALVGDSTNAVREGRSPSEAEVAKT
                     ITELVKAAKGRVAVTTFASNVARLRAVADAAKAAGREVVVVGRAMERVVQVARETGYL
                     DGVQSFRGADLYGHFPPDKVLALCTGSQGEPRAALSRIANDDHPQVTLNKGDCVIFSS
                     RTIPGNEKTVGGIINGLINQGIEVITDRTHLVHVSGHPRRDELRDMISWVRPQLLIPV
                     HGEALHLFEHAKLARAVGVPKVLICRNGDLVRLGPGDPGIIEQLPSGRLYKDGSILED
                     SKSRAVIERRRLAFAGCAFVAIAVTDKGELADDPEVDLVGVPEKNTAGEVIDEIVFDV
                     VVSTVEGLPRARRRDPDAMAESVRRAVRATINEQWGKKPLCYVHVMMV"
     gene            4305..4709
                     /gene="mce"
                     /locus_tag="VN968_08085"
     CDS             4305..4709
                     /gene="mce"
                     /locus_tag="VN968_08085"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HXL29233.1"
                     /translation="MLGRLNHVAIAVRDAEKAARIYGAVFGADISAAVPLPEHGVITV
                     FVTLPNTKIEFIQPLGEASPIAKFLERNADGGIHHICYDVPDIIAARDTLVREGARVL
                     GDGEPRIGAHGKPVLFLHPKDFSGALVEIEQA"
     gene            4716..4991
                     /locus_tag="VN968_08090"
     CDS             4716..4991
                     /locus_tag="VN968_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HXL29234.1"
                     /translation="MAYSISTAFAIYFVLWWIVLFLTLPFGVRSQHEDGEGAPGTDPG
                     APIVSHMGRKLIWTTVISAIIFASAMLAFNAGYFNIERLSKLMGIPL"
BASE COUNT          842 a         1503 c         1680 g         1015 t
ORIGIN      
        1 gcgctctggc tctatcgcaa ggtggtgttc ggcgcgctgg tcaagccgtc gctggcgagc
       61 atcaaggatc tcaccttccg cgaaagcctg acgctgatgc cgctggtgtt tctcaccatc
      121 ctgttcggga tttacccgaa gccggtactc gacatgtcgg cggcgtcggt gcagcaactc
      181 gttaccaatt acagccacgc ggtgactgcc atgaaggcag ccgcgctgct gcagtgaaca
      241 cagaagcgtt tttgagcgaa gtggaaaccg gttcgcatga agaaaacgcg tcaaacaaag
      301 aggatggagc ctcgtttctg aatcaatcag aaccgaggct ttgggtcagg acaacgcgcc
      361 atgagctttg agagtgcagg ttatcagtta ctgcccgtgc tgccggagct ggcgctggcc
      421 gcgggtgcca tggtgctgct gatgttaggg gcctatcgcg gcggcccaca gacgaccagc
      481 ctcgtcaccg gacttgcggt ttgcctgctg gtgcttgtgg gcgtcctgga actgctgttg
      541 ccggcgggaa agctgacgac gtttagcggc agcttcatcg tcgatgattt tgcccggttc
      601 ctgaagatat tgacgctgac cggctcggcg gcgaccttga ttctgtcggc ggaatttctc
      661 gccgatccat cccggcgcat cttcgaatac gccatcctgg tgctgctctc gacgctgggc
      721 atgatggtgc tgatctcggc cggcgacctg atcatgctct atctcggcct cgaactgatg
      781 tccctggcgc tttatgtggt cgcggcgagc aatcgcgaca acgcgaaatc cacggaggcc
      841 ggcctgaaat atttcgtgct gggcgcgctg tcgtcgggca tgctgctgta cggcgcgtcc
      901 ctggtctatg gcttcaccgg caccgtgagc ttcgccggca tcgccgcggc ggccaagacg
      961 ggcagcgtcg gcattgtgtt cggtttggtg tttctattgg ccgggctttg cttcaaggtt
     1021 tcggcggtgc cattccacat gtggacgccc gacgtctatg agggcgcgcc gacgccggtc
     1081 accgcattct tcgcctccgc accgaaggtc gcggcgttag cggtgttcac ccgcgtggcg
     1141 ctcaccgcat ttccggacat cgtgtcgcaa tggcagcaga tcgtggtgtt cgtcgcgatc
     1201 gcctcgatgg cgctgggatc gttcgctgcc atcggccaga agaacatcaa gcgactgatg
     1261 gcctattcgt cgatcggcca tatgggcttc gcactggtgg ggctggccgc cggaaccgtc
     1321 gagggcgcgc agggcgtact ggtttatatc tcgatctatg tggcgatgac gctcggcacc
     1381 ttcgcggtga tcctgaccat gaaacgcaac ggtcagcacg tcgagaccat cagcgacttc
     1441 gccggattgt cgcgcaccaa tccgctgctg gcattcttct ttgcgatgct gctgttctcg
     1501 ctggcgggca ttccgccgct ggcgggcttc tttgcgaaat ggtacgtgtt tgtggctgcc
     1561 atcaaggccg gattgtttac actggccgtg gtcggtgtgc tcaccagcgt ggtcggcgcg
     1621 ttttactatc tcaccatcat caaggtgatg tatttcgacg agccgctcgg ccagcttgac
     1681 ccgatgcgca tggaattgcg cacggtgctg gcggtcgcgg ggctgttcaa catcttcttc
     1741 tttgtctatc cggggccgct cgtcagcatg gcaacggtcg cggcgaagtc gctgttctaa
     1801 atgacatttt cgctcggtcc tcgagccgca tcggcgggat accggctcgc tgcattcgac
     1861 agcatcggct cgaccaacgc ggaagccatc gcgcgtgcgc gcggcggcga gcctggaccg
     1921 atgtggtttg tgacgtcgga acagacggcg ggacgtggac gacggcatcg accctggatt
     1981 gcgccgcgtg gtaacctcgc cagcagcatc ctcgaagtga tcgacgtttc gcccgcggtt
     2041 gcggcgacgc tgggctttgc tgcggggctt gcgctcgagg cggcgctgca aagagtaagc
     2101 gtcgaagcgt cgctgcgggc ggcgggatcg gaccatatga agttctcgct gaaatggccc
     2161 aatgatgtgc tggcggggcg acaaaagctc gccggcatcc tgctggaggc cgaggccgta
     2221 gcgggcgatc gcctggccgt cgtggtcggc atcggcacca acgtcatagc cgcgcccgag
     2281 gccacgccaa cgcctgcgac ttcgctggct gcgctggggg tcaatatcgg ggcggaggaa
     2341 ttgtttgccg tcctgtcgga ctcgtggacg gaatttcgtg gcatctggga ccggggccgt
     2401 ggcttcggtg aaatccggcg gctctggctg gagcgcgcgg ccgggctcgg gcatggggtc
     2461 gcgattcagt ccggcagcgc catcgtcgaa gggacattcg ataccatcga cgagcaaggc
     2521 tgcatgatcg tccggacagt agacggcaag cggatcccga ttgccgcggg cgacgtctat
     2581 ttcggctcgg tggcgtcggt aggggcaacc tgatggcgcg accggacgaa ctgacctttg
     2641 cgccgctcgg tggcgtcggt gagatcggca tgaacctgtc gatctacggc cttggcaacc
     2701 gccaccagcg ctcctggctc gcggtcgatc tcggcgtatc ctttggcgac gaggagcatc
     2761 tgccgggcat cgatctgatc atgcccgata tccgctttct cgaaaaggag cgcaagaatc
     2821 tcattggcct ggtgttgacc catgcccacg aagaccattt cggcgccatt atggatctgt
     2881 ggccgaagct gaaatgcccg atctatgcga ccaaattcag cgccgcgttg ttcgaggcca
     2941 aatgcgcatc ggagcgcaat ccgccgaaaa ttccggtcac ggtggtgccg tcgggcgggc
     3001 gcatcgatct cgggccgttc agtgtcgagt tcattccggt ggcgcattcg attccggaat
     3061 cccatgcgct cgcgatccat acctctgccg gcaccgtgtt gcacaccggc gactggaaga
     3121 tcgatccgac gccgatcatc ggcgtgccca ctgacgaacg gcggttgcgc gagatcggcg
     3181 acgccggcgt gctggcgctg gtcggcgatt ccaccaacgc cgtgcgggaa gggcgctcgc
     3241 cgtcggaggc tgaagtcgca aagaccatca ccgaactggt gaaggcggcg aagggccggg
     3301 tcgcggtgac cacgtttgct tccaacgtcg cgcggctgcg cgcggtcgcg gacgctgcga
     3361 aggcggccgg gcgcgaggtc gtggtcgtcg gccgcgccat ggagcgcgtg gtgcaggtgg
     3421 cgcgcgaaac cggctatctc gatggcgtgc agagtttccg tggcgccgac ctctacggcc
     3481 attttccgcc tgacaaggtg ctggcgctat gtaccggcag ccagggcgaa ccgcgcgcgg
     3541 cgctgtcccg catcgccaat gacgatcatc cgcaggtgac gctcaacaag ggcgattgcg
     3601 tgatcttttc ctcgcgcacc atccccggca atgagaagac ggtgggcggc atcatcaatg
     3661 gcctgatcaa ccagggcatc gaggtgatta ccgaccgcac ccacctcgtc cacgtctccg
     3721 gccatccgcg gcgcgatgag ctgcgcgaca tgatttcatg ggtgcgtcca caattgctga
     3781 tccccgtgca cggcgaggcg ctgcatctgt ttgaacatgc aaaactggcg cgggccgtcg
     3841 gcgtgccgaa ggttctgatc tgccgcaacg gcgatctggt caggctgggg ccgggcgatc
     3901 ccggcatcat cgagcagctg ccgtcgggac ggctctacaa ggatggctcg atcctggagg
     3961 attccaaatc gcgcgccgtc atcgagcggc gcagactggc cttcgccggc tgcgcgttcg
     4021 tggcgattgc ggtgaccgac aagggcgaac tggccgacga ccccgaggtt gatctggtcg
     4081 gcgtccccga aaagaatacg gccggagagg tcatcgacga gatcgtgttc gacgttgtgg
     4141 tgtcgacggt ggagggcctg ccgcgggcgc gccgccgcga tcccgacgcg atggcggagt
     4201 cggtgcggcg ggcggtgcgt gccacgatca acgagcagtg gggcaagaag ccgctgtgct
     4261 acgttcacgt tatgatggtc taggtttcag ggcggaggag tgtcatgctg ggccggctca
     4321 atcatgtggc gatcgcggtc agggacgccg agaaggccgc caggatctat ggcgcggtgt
     4381 tcggcgccga catctcggcg gcggtgccgt tgccggagca tggcgtcatt accgtgttcg
     4441 tgacccttcc caacaccaag atcgaattca tccagccgct cggcgaggca tccccgatcg
     4501 ccaaatttct cgagcgcaac gccgacggcg gcattcacca catttgctat gatgtgcccg
     4561 acatcatcgc cgcgcgcgac accctggtca gggagggcgc gcgggtgctc ggcgacggcg
     4621 agcccaggat cggtgcccac ggcaagcccg tgctgtttct gcacccgaag gatttttccg
     4681 gcgcgctcgt cgaaatcgaa caggcctgag ccgcgatggc ctacagcatc tcaaccgcgt
     4741 tcgcgatcta cttcgtgctg tggtggattg tactgttcct gacgctgccg ttcggcgtgc
     4801 gcagccagca cgaggacggc gagggtgcgc cgggtaccga tcccggcgcg ccgatcgtga
     4861 gccatatggg ccgcaagctg atctggacca cggtgatatc ggccatcatt ttcgccagcg
     4921 cgatgctggc cttcaacgcg ggctatttca atatcgagcg gctgtcgaag ctgatgggga
     4981 ttccgctctg accgctggat ggtgggggaa gaaagtgacg tttcaaagaa tcattttagg
//
LOCUS       DATNOI010000210         6586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_704388,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000210 DATNOI010000000
VERSION     DATNOI010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704388"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="VN968_08095"
     CDS             complement(<1..1535)
                     /locus_tag="VN968_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXL29235.1"
                     /translation="MAPIKFGVGQGVLRKEDDALIRGRGRYTDDHAPQAAMHALVLRS
                     PHAHAKFTINTSRARGLPGVATILTAEDVKDLGGLPCLFNLEVDPFTGPPYAILAHGV
                     VRHVGDAVAFVVADTIDQARDAVEAIEVEWTPLPAVIGVANAVRKGAPQVWPDHPGNV
                     LFDVPIGDKHATEAAFAKAHAVAEISVVNPRVVTNFMETRAAVAEYDAKRDHLTLTIG
                     SQGSHRLREILCGMVLKMPMEKMRVICPDVGGGFGTKLFPYREYALIAVAARKLHKTV
                     KWTADRADHFMGDAQGRDNVTTARMALAEDGKFLAMDVDLIGDMGAYLSTFGPYIPHG
                     GAGMLPGLYDIQTFHCRVRTVFTNTVPVDAYRGAGRPEAAYVVERLVDAAARKLGMTP
                     DAIRRKNFIAPRAMPYKTATGKIYDSGDFTAHMKRAMEIANWKEFPKRAKAAKKQGLV
                     RGIGLATYVEVCGTMGEETANVRLDPNGDVSILIGTQSSGQGHQTAYAQLVAEQFGIA
                     PERV"
     gene            complement(1628..3157)
                     /gene="murJ"
                     /locus_tag="VN968_08100"
     CDS             complement(1628..3157)
                     /gene="murJ"
                     /locus_tag="VN968_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HXL29236.1"
                     /translation="MLGRIFTVGGYTLLSRLTGLARDIMLAAILGAGPVADAFYVAWR
                     LPNNFRAIFAEGAFNTAFIPAYAHVHGKGGETSARLFADRIFTLLLLSQVVLLVVAWL
                     FMPQAMRILAPGFPEDSEQRRLAIELTRITFPYLLLITMVTLYGGMLNVMQRFASAAA
                     ASIFLNIAMMATLALAALFPTVGHAAAWGVLISGFLQYFLLAGDLARRGGLPRFAPLR
                     LDEDVRAFFRAIGPATLGSMGTQVAMFADTIIATFLPVGALSALYYAERLYQLPIGVI
                     GIAIGTVLLPEMSRRITAGDEDGAAASQRRAFEFTLLATVPFVAAFLTVPDVIMRAMF
                     ARGAFTTANAAAAGATLAAYAVGLIPFVLIRSAVATFYARKDTATPVKASLTGLTANV
                     LLKILLMGSLAQVGLALATAVGAWINLLLVVGLAVRAGYLDLDRALLQSLAKFVVSGA
                     VLGAALWLAARFAASHVLELSALRHEAALLLLIVVGTIVYAGSILLLFGWGWPRSLVR
                     S"
     gene            complement(3260..4411)
                     /locus_tag="VN968_08105"
     CDS             complement(3260..4411)
                     /locus_tag="VN968_08105"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXL29237.1"
                     /translation="MNQHLRPEPVPLIDLAAQRRRLGKSVDEAVSRVLTHCQFINGPE
                     VTQLEAALAAFSGAKHVVSCASGTDALLMVLMAKGVGRGDAVFCPSFTFCATGEAVAL
                     TGATPVFVDVDEETFNMDAGSLKRGIATAIKRGLKPRAVIPVDLFGQSADHDAIGRVA
                     EADGLFVLDDAAQAFGASYKGRRLGTFGLVTATSFFPAKPLGCFGDGGAIFTDDAELA
                     ETLRSIRVHGQGSDKYDNVRLGLTGRLDTLQAAILIEKLKIFEDEIAARNVVAERYAR
                     GLGNIVSVPRLASGCTSVWAQYTIRLPKGSDRDAFAAALKAQGIPTAIYYPKSMHQQT
                     AYRDFPVADGGVPISEALSDDVISLPMHAYLDEPTQERIIRAVRGALTA"
     gene            complement(4452..5471)
                     /locus_tag="VN968_08110"
     CDS             complement(4452..5471)
                     /locus_tag="VN968_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXL29238.1"
                     /translation="MISKGPASGGKAEAKRALRVGVIGAGVMGSNHARVLGGLPDTTL
                     VGVVDPLLAHRTRVMDLTGCRTFATLDELIAEGIDAVTVAAPTHLHHELALACIAREI
                     HVLVEKPIASSVEEGQEIVDAARRAGVTLMVGHVERFNPAVAAIKAAISGEDILSIAI
                     TRVGPFPPRMSNVGVVIDLAVHDIDLIRWFTESDIEEVQPQLSSAVAEREDIALLQFR
                     TASGVLAHINTNWLTPFKARNVTVATRGKYVMGDLLTRQVTECFGFKPDGSYSMRHLP
                     VGHDEPLRAELIAFLGAVRSGGMPAVTGDEGVASLEIAIRCLETPARPAATSAPLKGP
                     RRVVG"
     gene            complement(5662..>6586)
                     /locus_tag="VN968_08115"
     CDS             complement(5662..>6586)
                     /locus_tag="VN968_08115"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HXL29239.1"
                     /translation="ISGEVRAVVKFVEKPDPATAAGYIKSGYLWNSGNFMFRASVLLD
                     EYRNVDADSVRVVSDAVTKAGRDLGFVTLDPDSFAKARAISIDYAVMEKTSRAAVVPV
                     ACGWSDVGSWLAVWELSDKDAQGNAAQGAAVFEDSRNCNVSSDKALVALEGVDDLVVV
                     ATQDAILVSRQKDANGLKRLVAKLKTVAPDVTEDHLKVHRPWGSYQSIDTGDRHQVKR
                     IVVKPGNRLSLQKHHHRSEHWIVVRGTARVTVNELVKTVHENESIYIPIGAVHRLENP
                     GKILLELIEVQTGSYLGEDDIIRIEDDYRRT"
BASE COUNT         1259 a         2135 c         2076 g         1116 t
ORIGIN      
        1 acgcgctcgg gcgcgatgcc gaactgctcg gcgaccagtt gcgcgtaagc ggtctggtgg
       61 ccttgcccgc tcgactgcgt gccgatcaga atcgaaacgt cgccgttggg atcgagccgg
      121 acgttggcgg tctcctcgcc catcgtgccg caaacctcga cataggtcgc aagcccgatt
      181 ccgcgcacca gaccctgctt cttggcggcc ttggcgcgtt tcggaaattc cttccagttc
      241 gctatttcca tcgctcgctt catatgcgcg gtgaagtcac cggaatcata gatcttgccg
      301 gtggccgtct tgtagggcat cgcgcgcggc gcgatgaagt tcttgcgccg gatcgcatcc
      361 ggcgtcatgc cgagcttgcg cgcagctgcg tcgacgagcc gctcgaccac ataggccgcc
      421 tccggacgtc cggctccgcg ataggcatcg accggcacgg tgttggtgaa caccgtgcgg
      481 acgcggcagt gaaaggtctg gatatcgtag agaccgggca acattccggc gccgccgtga
      541 ggaatgtaag gcccgaaggt cgagagatag gcccccatgt cgccgatcag atcgacgtcc
      601 atcgcgagga acttgccgtc ctcggccagc gccatccgcg ccgtcgtcac gttgtcgcgg
      661 ccttgcgcgt cgcccatgaa atggtcggca cggtcggcgg tccacttgac ggtcttgtgc
      721 agttttcgcg cggcgaccgc gatcaatgca tattcgcggt agggaaacag cttggtgccg
      781 aacccgccgc cgacgtccgg gcagatcacc cgcatctttt ccatcggcat cttcaggacc
      841 atcccgcaaa ggatttcgcg caagcgatgg ctgccctggc tgccgatcgt caatgtcaaa
      901 tggtcgcgct tggcgtcgta ttcggcaacg gccgcgcggg tctccatgaa gttggtaacg
      961 acccgcggat tgacgaccga tatttcggcc accgcatgcg ctttggcgaa cgctgcctcg
     1021 gtcgcatgct tgtcgccgat cggcacgtcg aacagcacgt tgccgggatg gtctggccag
     1081 acctgcggcg cgcccttcct gaccgcattg gcgacgccga tcacggcagg cagcggcgtc
     1141 cattcaacct cgatcgcctc gaccgcatcg cgggcctgat cgatggtgtc cgccacgaca
     1201 aaggccacgg cgtcgccgac atggcgcacc acgccgtgcg ccaggatcgc atagggcgga
     1261 ccggtgaacg gatcgacttc cagattgaac aggcagggaa gaccgccgag atccttgacg
     1321 tcctctgccg tcaggatcgt cgccacgccc ggcaggccgc gggcgcggct cgtgttgatg
     1381 gtgaatttcg catgcgcatg cggcgagcgc agcaccagtg cgtgcatcgc agcctgcggc
     1441 gcgtggtcgt cggtgtagcg gccccgacct cgaatgagcg catcgtcttc cttgcgaagg
     1501 acgccttgac cgacgccaaa tttgatggga gccatcgtat ctccgtttcc ccagttttga
     1561 ttttccgggt ttcccgttgt gatttctggc catattgcag cggatcgcaa cgcggcgcaa
     1621 acggacttta gctgcgcacc agcgacctcg gccagcccca gccgaacagc agcaggatcg
     1681 aaccggcata gacgatcgtg ccgacgacga tcaggagcag aagtgctgcc tcatgccgca
     1741 acgcactcag ctccaggaca tgcgatgcag cgaatcgcgc ggcaagccac agcgcagccc
     1801 cgagcacggc accggacacg acaaacttcg ccagcgattg cagcagcgca cggtcgaggt
     1861 cgaggtaccc tgcccgcacc gcaagcccga ccaccagcag caggttgatc caggcgccga
     1921 ccgcggtggc aagcgccagg ccgacctggg ccagcgatcc cataagcaga attttcagca
     1981 ggacgttggc ggtaagcccc gtgagcgatg ccttcaccgg cgtcgcggtg tccttgcggg
     2041 cgtagaaggt cgcgaccgcg ctgcggatca gcacaaacgg gatcagcccg acggcgtagg
     2101 ccgcaagggt agcgccggcg gccgcggcgt tggccgtcgt gaacgcgccg cgcgcaaaca
     2161 tcgcgcgcat gatcacatcg ggcacggtga ggaatgcggc gacgaatggc accgttgcga
     2221 gcagcgtaaa ttcaaaggca cggcgctgcg acgccgccgc gccgtcctcg tcgcccgcgg
     2281 tgatccggcg cgacatttcc ggcagcagca cggtgccgat ggcaatgccg atcacgccga
     2341 tcggcaattg ataaagccgc tcggcgtaat acaacgccga caatgcgccg accggcagga
     2401 aggtggcgat gatggtatcg gcgaacatcg caacctgggt gcccatcgaa cccagcgtcg
     2461 ccgggccgat cgcgcggaag aacgcgcgga cgtcctcatc gagccgcaac ggcgcgaagc
     2521 gcggcaggcc gccacgccgg gcgaggtcgc cggccagcag aaaatattgc aggaagccgg
     2581 agatcagcac gccccaggcc gcggcgtggc cgacggtcgg aaacaatgcg gccagcgcca
     2641 gcgtcgccat catcgcgatg ttgagaaaaa tagacgccgc tgcggcactg gcaaaacgct
     2701 gcatgacgtt gagcatgccg ccataaagcg tcaccatcgt aatcagcaga agatagggga
     2761 acgtgatccg cgtgagctcg atcgcgagac ggcgctgttc ggaatcttcc gggaagcccg
     2821 gggcaagaat gcgcatcgcc tgcggcatga acagccacgc gacaacaagc aggacgactt
     2881 gcgacagcaa cagcagggta aagatgcgat cggcgaacag ccgtgccgag gtctcgccgc
     2941 ccttgccatg gacatgggca taagccggaa tgaaggccgt gttgaaggcg ccctcggcaa
     3001 agatcgcacg aaaattgttg ggcaggcgcc aagccacgta aaacgcgtcc gccaccggcc
     3061 cggcgccgag gatcgccgcg agcatgatgt cgcgcgcgag gccggtcagc cgcgaaagga
     3121 gcgtgtagcc gccgacggtg aaaatacgac cgagcatgcg ccgcttctac agcatgcggc
     3181 ggcgcttcaa aacggtgaaa tcagaagatt cgtcgtttgg gcatgatctg gtccgaaaac
     3241 cggcaaccac ttttcgggat catgccgtaa gcgcgccacg cacagccctg atgatgcgct
     3301 cttgcgtcgg ctcatcgaga taggcatgca tcggcaggct gatgacgtcg tccgacagcg
     3361 cctcgctgat cggcacgccg ccgtcggcga ccgggaaatc ccggtaggcg gtctgctgat
     3421 gcatcgattt cggatagtag atcgcggtcg gaatgccctg cgccttcaag gccgcggcaa
     3481 aagcgtcgcg gtcactcccc ttcggcagcc ggatggtgta ttgcgcccac accgaggtgc
     3541 atccgctggc aagacgcgga accgacacga tattgccgag accccgggca tagcgctccg
     3601 caacgacgtt gcgtgcggcg atctcgtcct cgaagatttt cagcttctcg atcaggatcg
     3661 ccgcctgcag ggtgtcgagc cgtccggtga gcccgagccg aacattgtcg tacttgtcgg
     3721 agccctggcc atgcacgcgg atgctgcgca gcgtctcggc gagttcggca tcgtcggtga
     3781 aaatcgcccc gccatcgcca aagcagccga gcggttttgc ggggaaaaag ctggtcgcgg
     3841 tgacaaggcc gaaggtgccg agccggcggc ccttgtagct cgcgccgaac gcctgggctg
     3901 cgtcatcgag cacgaacagg ccgtcagctt ccgcgaccct gccgatggcg tcatggtcgg
     3961 cgctttgccc gaacagatcg accggaatca ccgccctcgg cttgaggccg cgcttgatcg
     4021 cggtcgcgat gccgcgcttc agcgaacccg catccatgtt gaaggtctct tcatcgacat
     4081 caacgaacac cggcgtagca cccgtcaacg ccaccgcctc gccggtcgcg caaaatgtga
     4141 acgacggaca gaacactgca tcgccacggc cgacgccctt ggccatcagc accatcagca
     4201 gcgcgtcggt gccgctggca cagctcacga catgctttgc gccgctgaaa gccgcgagcg
     4261 cagcctcgag ctgcgtcacc tccggaccat tgatgaactg gcaatgggta agcacccggg
     4321 acaccgcctc atcgacggat ttgccgagcc ggcggcgctg cgcggcgagg tcgatgaggg
     4381 gtacgggttc tggacgcagg tgctggttca tggagccttg caggaatttg gaatgggaca
     4441 ttgacgcgcg attagccgac cacgcggcgc gggcccttga ggggtgcgga ggttgcggca
     4501 ggccgtgccg gagtttccag gcaacggatc gcgatttcaa ggctggcgac gccttcgtcg
     4561 ccggtcaccg cgggcatgcc gccggagcgc accgcgccga ggaacgcgat caattcggcc
     4621 cgcagcggct cgtcatggcc caccggcaga tgccgcatcg agtagctgcc gtccggcttg
     4681 aagccgaagc actcggtcac ctgacgggtg aggagatcgc ccatcacgta tttgccgcgg
     4741 gtggcgacgg tgacgttgcg cgccttgaac ggcgtcagcc agttggtgtt gatatgcgcg
     4801 agcacgccgg aggcggtgcg gaactgcaat agcgcgatat cctcgcgctc ggcgacggcg
     4861 ctggagagct gcggctgcac ctcctcgatg tccgactccg tgaaccagcg gatcagatcg
     4921 atgtcgtgca cggcgagatc aatcacgaca ccgacgttcg acatgcgcgg cggaaacggt
     4981 ccgacgcggg tgatcgcgat cgaaagaatg tcctcgcccg atatcgccgc cttgatggcg
     5041 gcgacggccg ggttgaaacg ttcgacatga cccaccatca gcgtcacgcc ggcccggcgt
     5101 gcggcgtcga cgatctcctg cccctcctcg accgaggacg cgatcggctt ttcgaccagg
     5161 acgtgaatct cgcgcgcgat gcaggcgagc gcaagctcgt gatggaggtg cgttggcgct
     5221 gcgaccgtca cggcatcgat accttcggcg atcaactcgt ccagcgtggc gaaggtgcgg
     5281 cagccggtca gatccatgac gcgcgtccga tgcgccagca gcggatcgac aaccccgacc
     5341 agcgtcgtat ccggcaaccc gcccagcacg cgggcatggt tgctgcccat gacgccggcc
     5401 ccgatgacgc cgacgcgaag cgcgcgtttt gcctcggcct tcccgcccga tgcgggcccc
     5461 ttggaaatca taaatcagcc ccgatcatgt ttcgacacca acatcccgtg gctctcctag
     5521 cacgttcgtc acaaacgtgg cgaatgccga gaggctctcc cggacacgtt ttgagtcaga
     5581 aaattacacc atcccgctac cggcccccgg cagcgaacat gatccaaaag aagcctgatt
     5641 cgaggcgaaa cgatgaattg ctcaggtgcg ccgataatca tcctcgatac ggatgatgtc
     5701 gtcctcgccg agatagctgc cggtctgaac ctcgatcagt tccagcagaa tcttgccggg
     5761 attttccagc cggtggaccg cgccgatcgg gatatagatc gactcgttct cgtgcacggt
     5821 cttgacgagt tcattgaccg ttacgcgcgc cgtgccgcgc acgacgatcc agtgctcgga
     5881 gcggtgatga tgcttttgca gtgagagccg gttgcccggc ttcacgacga tgcgcttgac
     5941 ctggtggcgg tctccggtgt cgatcgattg ataacttccc cacgggcggt gcaccttgag
     6001 atgatcctcg gtcacatcgg gtgctaccgt cttcagcttg gcgaccagcc gcttcagtcc
     6061 gttggcgtcc ttctgacgcg acaccagtat ggcgtcctgc gtcgccacca ccacgagatc
     6121 gtcgacccct tccagcgcga ccagcgcctt gtcgctcgaa acgttgcagt tgcgtgaatc
     6181 ctcgaacacc gcggcgccct gcgcggcgtt gccctgggca tccttgtccg acagttccca
     6241 caccgcaagc caggagccga catcggacca gccgcacgcc accggcacca ccgcggcgcg
     6301 cgacgtcttt tccatcaccg cgtagtcaat ggagatcgcc ctggcctttg caaacgaatc
     6361 cggatcaagc gtcacgaagc caagatcgcg cccggccttg gtcacagcat cgctcaccac
     6421 tcgcacgctg tcggcatcga cattgcgata ttcgtcgagc agaacagatg cgcgaaacat
     6481 gaagttgccg ctgttccaga gataaccgga cttgatgtaa ccggcggccg tcgccggatc
     6541 gggtttttca acaaatttga cgaccgcgcg gacctcgccg gaaatg
//
LOCUS       DATNOI010000211        18467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2327212,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000211 DATNOI010000000
VERSION     DATNOI010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18467
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2327212"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VN968_08120"
     CDS             complement(<1..729)
                     /locus_tag="VN968_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXL29240.1"
                     /translation="MTKMSKVRNLEIVACDAGWRNYHFVKITTEDGIVGWSEFDEGFG
                     SPGVGAVIQRLSARVIGQNAFQHERIYAELFSATRPGAGGVVAQALGAIENALLDAKA
                     KLLGVPCYELLGGKIRDRIRVYWSHCATWRINHPSWYKPPINDLDGVKAIGREVREKK
                     FTALKTNIFTYDAGKPQGWRPGFGSPFEPEINVDHKVLRDLRMHLEAIRDGAGPDVDI
                     LLDLNFNAKTEGYLKILRAIADLDM"
     gene            complement(775..1566)
                     /gene="soxA"
                     /locus_tag="VN968_08125"
     CDS             complement(775..1566)
                     /gene="soxA"
                     /locus_tag="VN968_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HXL29241.1"
                     /translation="MRSARAIFLAALLIAAGIGVSPLPAAEIPPDQRRSGYDFMTPDT
                     QAIQNDDTANPGMLWVLDGEALWKGKTGAAGKACADCHEDARASMKGVAARYPAFDRT
                     LGRPVNLEQRINMCRVQHQQTTPLAYESRDLLALTAFVAQQSRGDAIVTGADPQLEPF
                     VAKGRGLFMQRQGQLNLGCTNCHDDNWDKRLAGSAITQGHPTGYPLYRLEWQSLGSLQ
                     RRMRACMTGVRTQAYDYGAPELVELELYLMSRAQGMPMDSPAVRP"
     gene            complement(1563..1883)
                     /gene="soxZ"
                     /locus_tag="VN968_08130"
     CDS             complement(1563..1883)
                     /gene="soxZ"
                     /locus_tag="VN968_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HXL29242.1"
                     /translation="MASALINVPKKAKRGEIIEIKTLMSHIMETGYRRTATGEIIPRD
                     IITSFACRYNGVEIFRADLFPAIAANPFMTFFTVATESGKFDFEWIGDRGFSETASAS
                     ILVE"
     gene            complement(1883..2362)
                     /locus_tag="VN968_08135"
     CDS             complement(1883..2362)
                     /locus_tag="VN968_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxY-related AACIE arm protein"
                     /protein_id="HXL29243.1"
                     /translation="MHKQQGSTRRQFLGLAGGAAALSAVPIVTVRPADATPAMLAAAI
                     RNVVGEAEVRTGKVKLDVPPLVENGNTVPMTVNVFSPMTPEDYVKSIHVFNEKNPQPN
                     IGNFYLGPRAGRAQVSTRIRLADSQKVVAIARLSDDSFWSASVDIVVTLAACTEEVI"
     gene            complement(2379..2843)
                     /gene="soxX"
                     /locus_tag="VN968_08140"
     CDS             complement(2379..2843)
                     /gene="soxX"
                     /locus_tag="VN968_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HXL29244.1"
                     /translation="MPLASPAIQILGACLAAVAAALPGAASAAALRTYAIVGDAIPQS
                     LTGEPGDVTRGRALVVDRANTCILCHNGPFPEAKFQGDLAPGLGGAGGRWSEGQLRLR
                     LVDASRLNPATIMPSYYRVDGLDRVGPAWRDKPILSAEQIEDMVAYLASLRE"
     gene            complement(2830..4107)
                     /locus_tag="VN968_08145"
     CDS             complement(2830..4107)
                     /locus_tag="VN968_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL29245.1"
                     /translation="MDALRQMTRRNVVRGIAASAASLTLRCPAFAQSAARVVVIGGGF
                     GGASCARALKRIDPKLEVTLIEPNRTFIACPFSNEVIAGLRDIEAQRFSYDRISAEGV
                     TFVAQAAVKIDPQTRVVGLADGQSLSYDRLVLAPGIDIRFDALSGYDEEATARMPHAW
                     KAGEQTMLLRRQLEAMDDGGLVALAVPAGPSRCPPAPYERASLIAHYLKTRKPRSKIL
                     ILDAKDAIPQQRLFENAWQELYPGMIERVSLSQGGRVTSVDPATNTIVTDFGNYTAAV
                     ANVIPPQKAGRIAGIAGAVDNTGWCPIDPVSFASKLVPNIHVIGDACFGGVIPKSASA
                     ANAEARACAAAVANLIAGTAPPTPKLFGACYSTVAPGYAFAQSGTYQPRDGIFAEIEA
                     GGTSPVDAPREVRRRDADNAQSWFKTITVDAFG"
     gene            complement(4161..6338)
                     /locus_tag="VN968_08150"
     CDS             complement(4161..6338)
                     /locus_tag="VN968_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL29246.1"
                     /translation="MNQMPTFNRRAFVIGTAAVGAGLALGLDIPFGGPTVVRAADGSP
                     EVNAWVVIRPDDTVVIRIARSEMGQGTLTGLAQLVAEELECDWSKVTTEYPTPGQSVA
                     RKRAWGDFSTGGSRGIRTSQDYVRKGGATARMMLIQAAANDWKVPVAECSAANSVITH
                     APSGRTTTYGKVVEAAAKLPPPADVKLKDPKDWKLVGKGLKRLDTPDKVTGAMVYGID
                     VKLPGMLNAAIKDCPVTGGTLKSYDEAKIAGMKGVKKVVKVGDTAVAVVADTWWHAKT
                     ALDALPIVWDEGENAKVTSASIAQWLAEGLDSGPAYIGNQSGDAKAAFAGAVKKVEAV
                     YNYPYQNHATMEPMNATALYTSDKCEVWCGTQNGEAAFAAVLEGSGLPADKCDVHKQI
                     LGGGFGRRGQTDYVRQAVDIAKQMPGTPIKLLWSREEDMLRGRYHPVTQCKMTAALDA
                     DNNLTALHMRISGQSILFSLRPEALVNGKDPATFAGLNPDGDAALGYSIPNLLIEHSM
                     RNPHIIPGFWRGVNVNHNALYLECFMDELAHAAGQDPLEFRRKLLTKHPKNLAVLNAV
                     AEKIGWDKPAPQGVYRGLAQYMGYGSYVAAAAEISVTDGTKIKVHRIAAATDPGYAVN
                     PAQIERQIAGSFVYGLTGLFYGGCTVKDGRIEQTNFDTYNSMRIAEMPKVESIVMPSG
                     GFWGGVGEPTICVAGPAVLNAYFGATGKRIRSVPLRDQNITFA"
     gene            complement(6349..6798)
                     /locus_tag="VN968_08155"
     CDS             complement(6349..6798)
                     /locus_tag="VN968_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL29247.1"
                     /translation="MANLTINGKAFDVDVEADTPLLWAIRENAGLTGTKYGCGIAQCG
                     ACTVHIDGVAMRSCGVQVSEAVGKQITTIEGLAAEGALHKVQQAWINNDVPQCGYCQS
                     GMIMAVAALLKEKPKPTDQDIDEAITNICRCGTFQQVREAIHAAASV"
     gene            complement(7726..8625)
                     /locus_tag="VN968_08160"
     CDS             complement(7726..8625)
                     /locus_tag="VN968_08160"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL29248.1"
                     /translation="MQDLDWNDLRYILCLSRTGRLAGAARKLGVNETTVARRIARVEG
                     LLGVRMFERDAGVLRPTDSGQIVVRRAERIELDVDAVKDGVTSADSIAVGKVRVTAVP
                     LVLNYILIPALPDLLRSHTRLQLELAADHRNLSVTNREADIALRMARPDNEYRAVAHR
                     VGIVDYAVYGASAARPPLPWITYEASWSHLPHVRWMAKAVASDPDARISVVVNDSELA
                     LNAVRTGLGRSLLPCRIGDGVSEVSRLSGAKPVLSREMWLIIHPDLKQLARVRAVIAW
                     IERLFSERPVALQTKITDARRRI"
     gene            8757..9347
                     /locus_tag="VN968_08165"
     CDS             8757..9347
                     /locus_tag="VN968_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXL29249.1"
                     /translation="MSAVLSRRRLTTLAMFGLTAHVALLSMPMGTVQAQGMKPIQEPI
                     TGNEELGDLSQPQQALAQFYRAFNTRDLKMMDENFAHSDEVAIDNPLGGIRRGADEPH
                     KMYEGVFKSPADVHVEFWDYTIHRAGDVFWAVGRERGTYLDGEIVKNLSVRTTRIFQL
                     IDGRWRQMHHHGSIEDAKLLSDYQSVVRSPTPKTLK"
     gene            complement(9546..9743)
                     /locus_tag="VN968_08170"
     CDS             complement(9546..9743)
                     /locus_tag="VN968_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29250.1"
                     /translation="MLVLFGEFDNGISEDLVRGVYPALYPHAEIEQISNLRTLSDAGN
                     ADPFCNQAASSYFSISVKSCA"
     gene            9895..10875
                     /locus_tag="VN968_08175"
     CDS             9895..10875
                     /locus_tag="VN968_08175"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL29251.1"
                     /translation="MFSRYLKMAALCIGVTIIAAAGTASLMAQTYPAQPIKVIVGFGP
                     GSAADILARLVGKQMEVRLGQPIVVENRPGNSSMIAAETVARAAPDGYTLFMATIANT
                     LNPAETKSGFNLGKDLAPIALLGMVPNVLVAHPSVPANSLEELIALAKSKPESLTFGS
                     SGFATASYMAAELFNDKAGTKILTVPYQGGSNQAVSDLLSGRITLMFNVAATLAPHVE
                     AGKLKAFAVAQSTRASIMPDVPTLAEAGMTGYDAGIWIGLLAPPGTAPAIIEKLSAAA
                     NEALKTQDVETTLKRQGTDPRGGTPKEFADFIRADIEKWLTILARPGLGK"
     gene            11105..12874
                     /locus_tag="VN968_08180"
     CDS             11105..12874
                     /locus_tag="VN968_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXL29252.1"
                     /translation="MNGQAGRRTEVTRSGGEGMRRRVLAGLWVALLLAILGFLVIYPL
                     LTLLLGALTDTNPVVDGFSLRHLSIVNFLAVLANPNVAEALFNTLIACGGGTLIAVAI
                     GLAFSWIVVRTNTPFKGFIAAASILPLFAPPLVAGVAWTILGSPKTGLINTLFKWAGL
                     DWRVDFYSLWGLVFVFGIYYAPYVYMFTSSALRNMDPSLEEAAEISGASAFATLFSVT
                     FPLIMPAIVSGMLLSFIVMLGIYGIPAVLGAPTNISVLTTYIFKLTNWSPPLYNTAAA
                     VAIILMVVTGALVFLQQKVLSGRSYTTVAGKAYRPRNLDLGRWRWFTFGLGVVYLLVV
                     VILPSLALIVAAFRKFMFIRDAASLFDMRQYSLMHFYSIFDNPLTMRSIYNAVEVGVI
                     TAVVGGTLAFAIGYTIHRTSVAGRRGIDLVSTLPVAIPGLVIGVAYLWAWIGIPGGLY
                     GTIWILALAFIARFMPDTVKALSTSFLQIHRELEEAAWVCGKGMLGTIRTIVLPLARP
                     GVIASMTLLFVLAIRELGSSLFLYTSNTMVMSVLLLDYYEGGNLGKTAAFSLVQTILL
                     GVLIGGANLLSRGQAQGSVARTG"
     gene            12899..13939
                     /locus_tag="VN968_08185"
     CDS             12899..13939
                     /locus_tag="VN968_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXL29253.1"
                     /translation="MTKEFVAKIVAMAAAAGLGVALNAAPALAQAEFGSPELIAAARA
                     EGKLVYYTANFAEVEQQVIKAFNQRFPEIKVEMVRAPGGQLITRVKTEAAAGKLIADV
                     VDHSDRSLMQPLADMFQDYAPPNAADYNPDAQISPNLWPRATLVWSIAYNTELVKDPP
                     KTWMDLTKPEYDKMTGQVFAQSGGTTWTRIMFERQVLGEDYWAKQATTHPILYPSGAP
                     MADSLVRGEVAMGPLLYNAIYPKQKDGAPIKIFFPPEGAPVNPYATGIPKTAAHPNAA
                     KLFLNWCLSKEGQTFMIKELGNLTSLKEPPIYPEGFDPKIVKVWLPKFDDYVKLHAAW
                     VADWDKTFGYRQ"
     gene            13968..15071
                     /locus_tag="VN968_08190"
     CDS             13968..15071
                     /locus_tag="VN968_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL29254.1"
                     /translation="MTATLDVTDLRKQFAIGRPAIDGASFAVPAGEIVVLLGPSGCGK
                     TTTLRCVAGLEHPTSGEISIGGRVVSSPGRGILVPPRSRDLGMVFQSYAVWPHMTVRQ
                     NVVYPLKHRKIARAEAARKVDEVLELVGLSEYADRPVVALSGGQMQRVALARSIVYRP
                     QLLLLDEPLSNLDAKLRLRLRDDLRIILKQTGMTALYVTHDQAEAVVLGDRIGVMRDG
                     KLLQMGSPDEIYNRPADLFVANFTGATNEIAGTLIERNGMSGLADVGCGQRVEVALLH
                     AISAGEKIRIALRPENIALGKQGGSNAFNARVLDRRYQGTQTVYDIDLFGHRLEVLEL
                     GTAARHQVGAETSVCLPREGCWAYRDTGSSSYD"
     gene            15170..16012
                     /locus_tag="VN968_08195"
     CDS             15170..16012
                     /locus_tag="VN968_08195"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL29255.1"
                     /translation="MTIIDSQVHAYEANTPKRPWHSVPNWPDHVTGDEMVAAMDKVGV
                     DGTIFISAFSLYRYDASYAVEVQRAHPGRFAIVKPVDPDDPDVADVVADWKNTPGTVG
                     IRIMLTREAKRDPDDPGLDRILRAAVRHDFPVNMLCWANLDAGTALIDRHPDTRFIID
                     HLGILQPRVPPAPPQPWADLAKVLELAKRPNAVIKVSGACTLSREPYPFPDIWDPLAR
                     VFDAWGFDRCLWGTDWTRAFAVVNYEQAVEPFLKTSRLSDTERAMLMGGACAKAYGWS
                     PKKG"
     gene            complement(16089..17063)
                     /locus_tag="VN968_08200"
     CDS             complement(16089..17063)
                     /locus_tag="VN968_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL29256.1"
                     /translation="MWKVVTALVTLASLAATPDVRAQQWPTRPLTMINPFAAGGPNDV
                     LARLFAQRMGEILGQTIIIENVAGAGGMNGADRVAKAAPDGYTFLQGTVGTQAQNQTL
                     FKKPAYNSTTDFAPVALIVEAPLVLVARKDLRVKDMKEFVAYAKANKDKMQFASAGTG
                     SAIHLGCALMNMVTGLDIVHVPYRGANPAMQDLMSGRVDYLCDIITTAKPQIDGGAVK
                     AIAILAKERSPVLPNVPTAVEQGFDVDAYTWNAFFLPKGTPDAIVQKLNHATIEAMKT
                     PAVREKLESFGLKFVSDDRTTPEYLAKFVQSEIAKWAVPIKTSGVSVD"
     gene            complement(17097..>18467)
                     /locus_tag="VN968_08205"
     CDS             complement(17097..>18467)
                     /locus_tag="VN968_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HXL29257.1"
                     /translation="MTDWVPALASLPGPRYLAFVLALEADIAAGRVKPGMRLLPQRDM
                     AQRLELSVGTISKAYAEAEQRGLISGEVGRGTFVQRRRPEARGLGSSEATINLALNVP
                     PYTGEDDAIASVLAAIVADGELSNLLGYLPHQGLRRHREAMVTWLTSLGVTADADHLF
                     ITHGGQHALSIALGMVASPGDTVLTESYTYSGMLALSAQCGYRLHGVATDASGLVPES
                     LDRAFAETGARVLYCMPTLQTPTGSVMPLQRRHAIAEIVRKHDAYLLEDDAYGYLLSP
                     PLAPVSRLIPERSFYIVSFAKCLAPGLRIAAMIAPEAFRDRSINALRATGWMAVPVMA
                     EVVARLIANGGLARQARLKRDKAALRDAIVRRVLKEWLPAAFAAPGFHIWLPLPAGRT
                     LAALIAQAAQAGITLAPPGTLQRMEQESLGVRLCLGAPATEADLERALIEIRRILEMA
                     EAISFV"
BASE COUNT         3324 a         5777 c         5825 g         3541 t
ORIGIN      
        1 catgtcgagg tcggcaatgg cgcgcaggat tttgagatag ccttcggtct tggcgttgaa
       61 attcagatcg agcaggatgt cgacatccgg tccggcgccg tcgcggatgg cttcgagatg
      121 catccgcaga tcgcgcagga ccttgtggtc gacattgatc tcgggttcga acggcgagcc
      181 aaatcccggc cgccagccct gcggcttgcc ggcatcgtag gtgaagatgt tggtcttgag
      241 cgcggtaaat tttttctcgc gaacctcgcg tccgatcgcc ttgacaccat ccagatcgtt
      301 gatcggcggc ttgtaccagc tcggatgatt gatgcgccag gtcgcgcaat gcgaccagta
      361 gacccggatg cgatcgcgga ttttgccgcc gagcagctcg tagcagggca cgccgagcag
      421 ttttgctttc gcgtcgagca aagcgttttc gatcgcgccc agcgcctgcg ccaccacgcc
      481 accggctccg gggcgcgtcg ctgaaaaaag ctccgcatag atccgctcgt gttgaaatgc
      541 attctgtccg atcacgcgcg ccgagaggcg ctggatcacc gcgccgacgc cgggcgagcc
      601 aaaaccttcg tcgaattcgc tccagccgac aatgccgtct tcggtggtga tcttgacgaa
      661 atggtagttt ctccagccgg catcgcaagc gacgatttcg agatttcgta ccttcgacat
      721 ttttgtcatg acgttccctt attttcttga tggcaatcgt cgaactgcct gggctcacgg
      781 tcgtactgcc ggtgaatcca tcggcattcc ctgcgcccgc gacatcagat aaagctccag
      841 ctcgaccagt tccggcgcgc cgtaatcgta agcctgagtc cggacgccgg tcatgcaggc
      901 gcgcattcgg cgctgcagcg aacccagcga ctgccattcc agccggtaca gcggatagcc
      961 ggtcgggtgc ccctgcgtaa tcgcggatcc cgcgaggcgt ttgtcccaat tgtcgtcgtg
     1021 gcaattcgtg cacccgagat tgagctggcc ctgacgctgc atgaacaatc cgcgtccctt
     1081 cgcgacgaag ggttcgagtt gcggatcggc gccggtgacg atcgcatcgc cgcgcgactg
     1141 ctgcgcgaca aaggcggtga gcgccagcag gtcgcggctt tcgtaagcga gcggcgttgt
     1201 ctgttgatgc tggacgcgac acatattgat gcgctgttcc aggttgacgg gacggccgag
     1261 cgtcctgtcg aaggcgggat agcgggcggc aacgcccttc atgctggcgc gcgcgtcctc
     1321 atggcaatcg gcgcaggcct tgcccgcggc accggttttg cctttccaca acgcttcgcc
     1381 gtcgagcacc cacagcatgc cgggattggc ggtatcgtcg ttctggatgg cctgggtgtc
     1441 cggtgtcatg aaatcgtaac cggatcggcg ctgatccggc ggaatttcag cggcgggtag
     1501 cggcgagaca ccgataccgg ccgcgatcag cagcgccgca aggaatatcg cgcgcgcgga
     1561 tctcattcga ccaggatcga ggccgacgcg gtctcgctaa atcccctgtc gccgatccac
     1621 tcgaagtcga atttgccgct ctcggtcgcg accgtgaaga acgtcataaa gggattcgcc
     1681 gcaatcgccg ggaacagatc ggcgcggaaa atttccacgc cgttgtaacg gcaggcaaaa
     1741 ctggtgatga tgtcgcgcgg gatgatctcg ccggtggcgg tgcggcggta gccggtctcc
     1801 atgatatgcg acatcagcgt cttgatctcg atgatctcgc cgcgctttgc ctttttcgga
     1861 acattgatca gcgccgaggc catcagatca cctcttcggt gcaggcggcg agcgtcacga
     1921 cgatgtcgac gcttgccgac cagaacgagt cgtcggacag ccgcgcgatg gcgacgacct
     1981 tctggctgtc ggcgaggcga atgcgggtgg aaacctgcgc gcggccggcg cgcgggccga
     2041 gatagaaatt gccgatattc ggctgcgggt tcttctcgtt gaagacgtgg atgctcttga
     2101 cgtaatcttc cggtgtcatc gggctgaaaa cattcacggt catcggcacc gtgttgccgt
     2161 tttcgaccag cggcggcacg tcaagcttga ccttgccggt ccgcacctcg gcttcgccca
     2221 ccacgttgcg gatcgcggcg gcgagcatcg cgggggtcgc gtccgccggc cgcactgtca
     2281 cgatcgggac ggcactcaac gcagccgcgc cgccggcgag gccgaggaac tggcggcgcg
     2341 tggaaccttg ttgtttgtgc atcgcgggtt ctgctttcct attcgcgtaa gcttgcgaga
     2401 tacgccacca tatcctcgat ctgctcggcc gacaggatcg gcttgtcgcg ccatgccggg
     2461 ccgacgcgat cgaggccgtc cacgcgataa taggacggca tgatggttgc gggattgagg
     2521 cgggaggcgt cgacgaggcg gagccgaagc tggccttcgg accagcggcc accggcaccg
     2581 ccgaggccgg gcgcgagatc gccctgaaat ttcgcttccg gaaacggacc gttgtgacag
     2641 aggatgcagg tattggcgcg gtcgacgacc agtgcacggc ctcgcgtgac gtcacctggc
     2701 tcgcccgtca gcgattgggg aatcgcatct cccacgatcg cataggtccg caacgccgcc
     2761 gcgctggccg cgccgggcag ggcggcggcc acggccgcga ggcacgcgcc gagaatctgg
     2821 attgccgggc tagccaaagg catccaccgt gatcgtcttg aaccaggact gcgcattgtc
     2881 agcgtcgcgc ctacgcacct cgcgcggcgc atcgaccggg ctggtgccgc cggcttcgat
     2941 ctcggcgaat atgccgtccc tgggctggta ggtgccggat tgcgcgaagg cgtagccggg
     3001 tgcgaccgtg ctgtagcagg cgccgaatag ttttggcgtc ggcggggctg tgcccgcgat
     3061 caagttcgcc accgcggcgg cgcatgccct ggcttcggca ttcgcggccg acgccgattt
     3121 agggatgacg ccaccgaagc aggcgtcacc gatcacgtga atatttggca caagcttcga
     3181 ggcaaagctg accggatcga tcgggcacca gccggtattg tcgaccgcgc ccgcgatgcc
     3241 ggcgatgcgg ccggccttct gcggcggaat gacgttggcg accgctgccg tgtagttgcc
     3301 gaaatcggtg acgatggtgt tggtcgcggg atcgaccgac gtcacccggc cgccctgcga
     3361 cagcgacacc cgctcgatca tgccgggata gagttcctgc caggcgttct cgaacaggcg
     3421 ctgctgcgga atggcgtcct tggcgtcgag aataaggatt ttggagcgcg gctttcgcgt
     3481 tttcagataa tgggcgatca ggctggcccg ttcataaggc gccggcgggc accgcgaggg
     3541 gcctgcaggc acggccagcg ccacaaggcc gccgtcgtcc atggcttcga gttgccggcg
     3601 caacagcatg gtctgctcgc ccgccttcca ggcatgcggc attctggccg tggcttcctc
     3661 gtcatagcct gatagcgcgt cgaacctgat atcgatgccg ggcgccagca ccagacggtc
     3721 atagctgagc gattggccgt cggcgagacc gacaacgcgt gtttgcgggt cgatcttcac
     3781 cgccgcctgc gcaacgaagg tcacgccttc ggcgctgatc cggtcatagc tgaatcgctg
     3841 cgcttcgatg tcccgcaagc ccgcgatcac ctcgttgctg aaggggcagg cgataaaagt
     3901 tcggttcggc tcgatcaggg tcacctcaag tttcggatcg atccgcttga gcgcccgcgc
     3961 gcagctggcg ccgccaaagc caccgccgat cacgaccacg cgcgccgcgg attgtgcgaa
     4021 cgcgggacag cgaagcgtca gcgatgcggc tgacgccgcg atgccacgaa cgacgtttct
     4081 tcgcgtcatc tgccgcagcg cgtccatgag caatttttcc gaaaatggag cggcggcccc
     4141 aaaggccgcc gctcgaaaca tcaggcgaag gtaatgttct ggtcgcgcag cggcaccgag
     4201 cggatgcgct tgccggtcgc cccgaagtag gcgttcagca ccgccggtcc cgcgacgcag
     4261 atggtcggct cgccgacgcc gccccagaag ccgccgctcg gcatcacgat cgattccacc
     4321 ttcggcatct cggcgatccg catcgaattg taggtgtcga agttggtctg ctcgatgcga
     4381 ccatccttga ccgtgcatcc gccatagaac agcccggtca ggccgtagac gaaggagccc
     4441 gcgatctggc gctcgatctg cgccggattg acggcgtagc ccgggtcggt ggcggcagcg
     4501 atccgatgca ccttgatctt ggtgccgtcg gtcacggaga tttccgccgc cgccgcgacg
     4561 tagctgccat agcccatgta ctgggcgagg ccgcgataga cgccctgcgg cgccggcttg
     4621 tcccagccga ttttttcggc gacggcattg agcacggcaa ggttcttcgg atgcttggtc
     4681 aagagcttgc gacggaactc gagcgggtcc tggccggcgg catgcgcgag ttcatccatg
     4741 aagcattcga gatagagcgc gttatggttg acgttcacgc cgcgccagaa gcccgggatg
     4801 atgtgcggat tgcgcatcga atgttcgatc aggagattgg ggatcgaata tcccaacgcg
     4861 gcatcgccat cgggattgag gccggcgaac gtcgccggat ccttgccgtt gacgagcgcc
     4921 tccggccgca ggctgaacag gatcgactgg ccggagatgc gcatgtgcaa cgcggtcagg
     4981 ttgttgtcgg catcgagcgc cgcggtcatc ttgcactgcg taactggatg atagcgtccg
     5041 cgcagcatgt cctcttcacg cgaccacaac agcttgatcg gcgtgcccgg catctgcttg
     5101 gcgatgtcga cggcctgccg gacatagtcg gtctggccgc gccggccgaa gccgccgccg
     5161 agtatctgct tgtggacgtc gcatttgtcc gcgggcaggc ccgatccctc cagcactgcg
     5221 gcgaaagcgg cctcgccgtt ttgcgtgccg caccagacct cgcatttgtc cgatgtgtag
     5281 agcgcggtgg cgttcatcgg ctccatcgtg gcgtgattct ggtagggata gttgtagacc
     5341 gcctcgacct tcttcacggc gccggcaaat gctgccttgg cgtcgccgct ctgattgccg
     5401 atataggcgg ggcctgaatc caggccctcc gccagccatt gcgcgatcga cgcgctggtg
     5461 accttggcgt tttcgccctc gtcccagacg atcggcaagg cgtcgagcgc ggtcttggcg
     5521 tgccaccagg tgtcggccac gacggcgacg gcggtatcgc cgaccttcac caccttcttg
     5581 acgcccttca tgccggcgat cttggcctcg tcatagctct tcagcgtgcc gccggtgacg
     5641 gggcaatcct tgatcgcggc gttgagcatg ccgggcagct tgacgtcgat gccatagacc
     5701 atcgcgccgg tgaccttatc aggtgtgtcg agccgcttca gtcccttgcc gaccagcttc
     5761 cagtccttcg gatccttcag cttgacgtcc gccggcggcg gcaatttcgc ggcggcctcg
     5821 accaccttgc cgtaggtcgt ggtgcggccc gacggcgcat gcgtgatgac gctgttggcg
     5881 gcgctgcatt cggccaccgg caccttccag tcgttggccg cggcctggat cagcatcatg
     5941 cgcgcggtcg cgccaccctt gcggacatag tcctgcgagg tccggatgcc acggctgccg
     6001 ccggtggaaa aatcacccca ggcgcgcttg cgggcgacgc tctggccggg ggtcggatat
     6061 tcggtagtga ccttcgacca gtcgcattcc agttcttcgg cgacgagctg ggcgaggccg
     6121 gtgagggtgc cttgtcccat ttcggaacgg gcgatgcgaa tgacgacggt gtcgtcgggc
     6181 cggatcacca cccaggcgtt gacctcgggc gagccatcgg cggcgcgcac cacggtcggg
     6241 ccaccgaagg gaatatcgag gccgagggcg aggccggcgc cgacggcggc ggtgccgatc
     6301 acgaaggcgc ggcgattgaa tgtgggcatc tggttcatgg cggcctcctc aaacgctcgc
     6361 ggcggcatgg atcgcctcgc gcacctgctg gaaggtgcca cagcggcaga tgttggtgat
     6421 ggcctcgtcg atatcctgat cggtcggctt cggcttctcc ttgagcaggg ccgcgaccgc
     6481 catgatcatt ccgctctggc aatagccgca ttgcgggacg tcattattga tccaggcctg
     6541 ctgcaccttg tgcagcgcgc cctcggccgc cagcccctcg atggtggtga tctgcttgcc
     6601 gacggcctcg ctgacctgca cgccgcagga tcgcatcgcg acaccgtcga tgtgaacggt
     6661 gcaggcgccg cattgtgcaa tgccgcaacc gtatttggtg ccggtcaggc cggcgttttc
     6721 ccggatcgcc cataacagcg gggtgtccgc ttcgacgtcc acgtcgaaag ctttgccatt
     6781 gattgtgagg tttgccatcg ctgatcccct gattagctca atccatcgat tgaacttcag
     6841 gggaatgctg gactgaaaat tggaacggtt caaatcaaga ttctgcgttt gcagcgcgga
     6901 agataattga tcggctggtg ttgcggcgct gttccgggag gagcctccgg catctctggc
     6961 gcatacgcca caagccgtct gtcaccaccc gcccgccgtc atgccccgcg aaagcggggc
     7021 atccaatacg tcgcggccta tcgtttccgt cacccccgtc tctgtaatat tggatcgccc
     7081 gctttcgcgg gcgatgacag ccgagagtaa atctttcaga cacagcttcg cgatctcgcc
     7141 gcaggtctcg cgcgagtttt gctctggact tcccgccctc tccaatcaga gggcgcaggg
     7201 aatgccgggc gcccgatgcg cccgcagccg cgcgtgtagt ggtagtaaac acgcgcgtta
     7261 gtcaccacgg tcacaccgga aaacgcccgg cattccccgc gcaatggttt taacggtttc
     7321 cttcgtgctc tccccggtga ccgggcttgt ttgccaccgt catcggcggt tgcccgccga
     7381 cttgacacca gcgtcggggt gtcaggacca cacgacttcg ccgtccgctt cagcgccgtt
     7441 cgtcaaaggc gtatccgcgt ccaccgcatc ccgtcccgcg gccgtgacga tcgcgtgtcg
     7501 cccctctgtg ggacgggaca gggcgagctt gtagagatga tttgcccgac ggggaaagcg
     7561 caatattttt gcaaacaggg ctggacacgc gatccacctg acagcccgtc gggcaaatca
     7621 gtcgacgtgt acgaccaggt cgcgacgcga ttgcggtgct ttccgtgact cccggtgtgc
     7681 cgtgcggtga tcgaatgagg cggcggtctt gcgcctcggc gaggtttaga tgcgacggcg
     7741 cgcgtccgtt atttttgtct gtagtgccac gggccgttca gagaacaacc gctcgatcca
     7801 tgctatcacc gctctgaccc gcgccaattg cttgaggtcg ggatggatga tcaaccacat
     7861 ttcacgagag agaacaggct ttgcgccgct caatcgggag acttcggaaa cgccatcccc
     7921 gatacggcaa ggcagcagcg agcggcccaa gcccgtacgg accgcgttta aggctaattc
     7981 cgagtcattg acgacgacgg atattcgcgc gtccggatcc gatgcgacgg ctttcgccat
     8041 ccaacgtaca tgcggcaagt gcgaccagct ggcctcatag gtgatccaag gcagaggggg
     8101 acgagccgcc gacgcaccat acaccgcgta atcgacgatg ccgacacggt gtgcgaccgc
     8161 gcgatattca ttgtccggtc gcgccatacg caacgcgatg tcggcttccc ggttggtgac
     8221 gctgagattg cgatgatcgg ctgcaagttc gagctgcaac cgcgtatgcg atcggagcaa
     8281 atccggcaat gcggggatca gaatatagtt tagcaccaac ggcaccgctg ttacgcgcac
     8341 tttgccgacc gcaatactgt cggcgctggt cacgccgtct ttgactgcgt cgacatcaag
     8401 ttcgatgcgc tcggctcgac gaacgacgat ttgcccactg tcggtcggcc taagaacacc
     8461 tgcatcgcgc tcgaacatgc ggactcccaa cagtccctca acgcgcgcaa tgcgccgcgc
     8521 gaccgtggtc tcattgacgc caagcttccg cgccgcgcca gcaagacggc ccgtgcgcga
     8581 caggcacaga atgtagcgta ggtcgttcca gtccagatcc tgcatttttg cagtctagta
     8641 cagcaaagat acctgtcgag cagcattttg ctgaagatag ggtcaataag gcggtgcgcc
     8701 gtcaccactc gcggagtgca acaaattgcc aacaatgctt aggagaggag aagcccatgt
     8761 cggcggttct atcccgtaga aggctaacca cattagccat gttcggtttg acagcccatg
     8821 tcgcgttgtt atcgatgccg atgggtacgg tgcaagctca aggcatgaaa ccgatacagg
     8881 agcccatcac aggtaacgag gaactcggag atcttagcca gcctcaacag gcattggcgc
     8941 aattctaccg cgcgttcaat actcgcgatc tgaagatgat ggacgaaaat ttcgcccact
     9001 ccgatgaagt cgcgattgat aaccctctcg gaggaattcg acggggcgcc gatgagcccc
     9061 acaagatgta tgagggggta ttcaagagtc cggcggacgt tcacgttgag ttttgggatt
     9121 ataccattca tcgcgctggc gatgtatttt gggccgtggg gcgggaacgt ggcacgtacc
     9181 tcgatggtga aatcgtaaaa aatctaagcg ttcgcacgac ccgaattttt caactgatcg
     9241 atggtcgctg gcggcaaatg catcatcacg gatctatcga agacgcgaag cttcttagcg
     9301 actatcagag tgtggtgcga tccccaaccc cgaaaacgct aaagtaaccc gactggaggc
     9361 aatcatatca cgacggtttg ccaaagttcg ttgagcgccc gcgcccttac cgcggacgcg
     9421 cgaccgcctg ctaccttttg gccctcgcgc gatcacctcc gcttgggatg caatgcgacc
     9481 atgctcaaat gaatagaccg gagccgctgg cgaagaatgg acaggcggac cggagcgcac
     9541 cgagatcacg cgcagctttt tacgctgatc gagaaataag agcttgccgc ctggttgcaa
     9601 aatggatcgg cgtttcctgc atcggataat gtccgcaaat tgctgatctg ctcgatttcg
     9661 gcatgtggat agagcgccgg atagacgccc cgcacaagat cctcgctgat gccgttgtca
     9721 aactcaccga acagcacgag catgggcgcc ttcttccgcc ggccgaattg aagtaaaact
     9781 tgccgccggt ttagcctggc gcaaggaaac ggccaagctg cgggaccggc atccgcaagc
     9841 gtcgacaaag ccatatgcgg atagaccgca aagatcgcgc aatcaggggg gacgatgttc
     9901 agccgctacc tcaagatggc cgcgctttgt atcggcgtaa cgatcatcgc ggccgcgggc
     9961 acggcctccc tcatggcgca aacctatccc gcgcagccga tcaaggtcat cgtcggattc
    10021 gggcccggtt ctgccgcgga tatcctagcc cggcttgttg gcaagcagat ggaagtccgt
    10081 ctcggtcaac cgatcgtggt cgaaaatcgg ccgggtaaca gcagcatgat cgccgctgaa
    10141 accgtggcgc gcgccgctcc cgatggatac acgcttttca tggcgacgat cgccaacacg
    10201 cttaatccgg cggagacgaa gtcgggtttc aatctcggca aggaccttgc gccgatcgct
    10261 ctgctcggca tggttcccaa cgtgctggtg gcgcatccat cggtcccggc caacagtctg
    10321 gaagagttga tcgcgctggc caagagcaag cccgaaagcc tgacattcgg ttcctccggc
    10381 tttgcaaccg cgagttacat ggcagccgag ttgttcaacg acaaagcggg caccaagatc
    10441 ctgacggtcc cttatcaagg cggaagcaat caagccgtct ccgacctgct ctctggccgc
    10501 atcacgttga tgttcaatgt ggctgcgacc ctggccccgc atgtcgaggc aggcaagctg
    10561 aaggccttcg ccgtggcgca gtcgacgcgc gcatcgatca tgccggacgt tccgacactc
    10621 gccgaggcgg gaatgacagg ctacgatgct ggaatctgga tcgggctcct tgccccgccc
    10681 ggaacggccc ccgcgatcat cgagaagctg tcggccgctg cgaacgaggc gttgaaaact
    10741 caggatgtag agacgaccct gaagcggcaa ggtaccgatc cgcgtggcgg cacgccgaag
    10801 gaatttgccg acttcatccg agccgacatc gaaaagtggc tcacgatact tgcgcggccc
    10861 ggccttggga aatgaagccc gggcggattt ggcaagtctt ggtaagtctt gggcaagtcg
    10921 tgctcgtcga caagggtctg atgacaccgt cgcagaacgt ccaatcgaca tccagcggca
    10981 ttctgacgct tcttccgaca accgactttc tgctactttc cgcccgccac gcgatcgact
    11041 aggcttgttg tgcggtgcgg ccagaataac aaagaaggct cgccggtgag gggaaaaaag
    11101 cgctttgaac ggacaggcag gccgccgcac cgaggtcacg cgcagcggtg gtgagggcat
    11161 gcgccggcgc gtattggcgg gcctgtgggt tgcgctattg ctggcgatcc tgggcttcct
    11221 cgtcatctat ccgctgctca cgttgctgct gggcgccctg accgacacca atccggtggt
    11281 cgacggcttc agcctgcgcc acctctcgat cgtcaatttt ctcgccgtgc tggctaaccc
    11341 gaacgtcgcc gaggcgctgt tcaacacact gatcgcctgc ggcggcggaa ccctgatcgc
    11401 ggtggcgatc gggctggcgt tctcctggat cgtcgtgcgc accaacacgc cgttcaaggg
    11461 ttttatcgcg gcggccagca tcctgccgct gttcgcgccg ccgctggtcg cgggcgtggc
    11521 atggaccatc ctcggttcgc caaaaacggg cctgatcaac acgctgttca aatgggcggg
    11581 tctcgactgg cgcgtggatt tttattcgct gtggggattg gtgttcgtat tcggcatcta
    11641 ttacgcgccc tacgtctaca tgttcacctc ctcggcgctg cgcaacatgg atccgagcct
    11701 ggaggaggcc gccgaaattt ccggcgccag tgcattcgcg acgctgttct cggtgacgtt
    11761 cccgctgatc atgccggcga tcgtgtccgg catgctgctg tcattcatcg tgatgctcgg
    11821 catctacggc attccggcgg tgctgggcgc gccgaccaat atcagcgtgc tgacgactta
    11881 cattttcaag ctcaccaact ggtcgccgcc gctgtacaat acagcggccg cggttgcgat
    11941 catcctgatg gtggtcaccg gcgcgctggt gtttctgcaa caaaaagtgc tgagcggacg
    12001 cagctacacc acggtcgccg gcaaggccta ccggccgcgc aatctcgatc tcgggcgctg
    12061 gcgctggttc actttcgggc tcggcgtcgt ctatctgctg gtggtggtga tcctgcccag
    12121 tctcgcactg atcgtggcgg cgttccgcaa attcatgttc atccgcgatg ccgccagcct
    12181 gttcgacatg cggcaatatt cgctgatgca tttctacagt atcttcgaca acccgctgac
    12241 gatgagatcg atctacaatg cggtcgaggt cggcgttatc accgcggtgg tcggcggtac
    12301 gctcgccttt gcgatcggct acaccatcca tcgcaccagc gtcgcgggcc ggcgcggcat
    12361 cgatctggtc tcgacgttgc cggtggctat ccctggcctc gtgatcggcg tcgcttattt
    12421 gtgggcctgg atcggcattc ccggcggact ctacggcacc atctggatcc tggcgctggc
    12481 tttcattgcg cgcttcatgc cggatacggt caaggcgctg tcgacttcgt tcctgcaaat
    12541 ccatcgcgag ctcgaggaag cggcctgggt ctgcggcaag ggcatgctcg gcaccatccg
    12601 aaccatcgtg ctgccgttgg cgcggccggg cgtgatcgcc tcgatgacgc tgttgttcgt
    12661 gctggcgatc cgcgaactgg gctcgtcgct gtttctctac accagcaaca ccatggtgat
    12721 gtcggtgctc ttgctcgact actacgaagg cggaaatctc ggcaagaccg ccgccttcag
    12781 tctggtgcag acgatcctgc tcggcgttct catcggcggc gcgaacttgc tgtcgagggg
    12841 gcaggctcag ggcagtgtcg cccgcaccgg ataaatgaaa ccaaggggag gattggcaat
    12901 gaccaaagaa tttgtcgcca agatagtcgc catggccgct gcggccggtc tcggcgtcgc
    12961 gttgaacgcc gcaccggcgt tggcgcaggc ggaattcggc tcgcccgaac tgatcgccgc
    13021 ggccagggcg gaaggcaaac tggtctacta cacggcgaat ttcgccgagg tcgaacagca
    13081 ggtgatcaag gcgttcaacc agcgctttcc ggagatcaag gtcgagatgg tgcgtgcgcc
    13141 gggcggccag ctcatcaccc gcgtgaagac cgaggccgcg gccggcaagc tgattgccga
    13201 cgtggtcgat cactccgatc gctcactgat gcagccgctg gccgacatgt tccaggatta
    13261 tgcgccgccg aatgccgccg actataatcc cgatgcgcag atttcgccga acctgtggcc
    13321 tcgcgccacg ctggtctggt cgatcgccta caacacggag ttggtaaaag atccgccaaa
    13381 aacctggatg gatctgacca agcccgaata cgacaaaatg accgggcagg tgtttgccca
    13441 gtccggcggc accacctgga cccgcatcat gttcgagcgt caggtgcttg gcgaggatta
    13501 ctgggccaag caggcgacga cccaccccat tctctatccg tcgggcgcgc cgatggcgga
    13561 ctccttggtg cgcggcgagg tcgcgatggg gcccttgctc tacaatgcga tctacccgaa
    13621 acagaaggac ggcgcgccga tcaagatttt cttcccgccc gaaggcgcgc cggtcaatcc
    13681 ctatgccacg ggaattccga agaccgcggc gcatcccaat gccgcaaagc tgttcctcaa
    13741 ctggtgcctc tcgaaagagg gtcagacttt catgatcaag gagctcggca acctgacgtc
    13801 gctgaaggag ccgccgatct atccggaagg tttcgatccc aaaatcgtaa aagtctggct
    13861 gcccaagttc gacgactatg tgaagctgca cgcggcctgg gttgcggact gggacaagac
    13921 cttcggctac cggcagtgat cgtcgcccgc atgaagtctt tgaaaccatg acggcgacgc
    13981 tcgacgtcac cgacttgcgc aagcagtttg cgatcggccg gccggccatc gacggcgcca
    14041 gctttgccgt gccggccggc gagatcgtgg tgctgctcgg tccgtccggc tgcggcaaga
    14101 ccaccacctt gcgctgcgtc gccgggctcg agcatccgac gtcgggcgaa atcagcattg
    14161 gcgggcgcgt ggtatcgtcg cccgggcgtg gcattctggt gccgccgcgc tcgcgcgacc
    14221 tcggcatggt gtttcagtcc tatgcggtct ggccgcacat gacggtgcgg cagaacgtgg
    14281 tctatccgtt aaaacaccgc aaaatcgcgc gcgccgaggc cgcccgcaag gtcgacgaag
    14341 tgctggaact ggtcggcctg tcggaatatg cagaccgccc ggtggtggcg ttgtcgggcg
    14401 ggcagatgca gcgcgtcgcg ctggcgcgca gcatcgtcta ccggccgcaa ctgctgttgc
    14461 tcgacgaacc cctgtccaat ctcgatgcca aattgcgcct gcggcttcgc gacgacctgc
    14521 gcatcatcct caagcagacc ggcatgaccg cgctttacgt cacccatgac caggccgagg
    14581 cggtcgtgct tggcgaccgc atcggcgtga tgcgtgacgg caagctcttg cagatgggat
    14641 cgccggacga aatctacaac cggccggccg atctgttcgt cgccaatttc accggcgcca
    14701 ccaacgagat tgcgggcacc ttgatcgagc gcaacggcat gtccggactg gccgacgtcg
    14761 gctgcggcca gcgcgtcgag gtggcgctgc tgcacgcgat atcagccgga gaaaaaatcc
    14821 gcatcgcctt gcggccggaa aacatcgcgc tcggcaaaca gggcggcagc aatgccttca
    14881 acgcccgcgt cctcgatcgc cgctatcagg gcacccagac ggtctacgat atcgacctgt
    14941 tcggtcatcg gctggaagtc ctcgaactcg gcaccgcggc ccgccatcag gtcggggccg
    15001 agacgtcggt ttgccttccg cgggaagggt gctgggcgta tcgtgatacg ggatcatcgt
    15061 cctacgatta gcggcaacag cgggccccaa gcgagttggc ggcgccgttg cctctggaca
    15121 cgaacctgct gcaacgtcca aactcgcttg gcacagagga gccgccccga tgacgattat
    15181 cgattcccag gtccatgcct atgaggcgaa cacgccgaag cgaccttggc acagcgtgcc
    15241 gaactggccc gatcacgtca cgggagacga gatggtggcg gcgatggaca aagtcggcgt
    15301 cgacggcacg atcttcatct ccgccttttc cttgtaccgc tacgacgcca gctatgccgt
    15361 ggaagtgcaa cgggcccatc ccggccggtt cgccatcgtc aagccggtcg acccggacga
    15421 tcctgatgtg gccgatgtcg tcgccgactg gaagaatacg ccgggcacgg tcggaatccg
    15481 catcatgctg acaagggagg ccaagcgcga tcctgatgac ccgggcctcg accggatcct
    15541 gcgcgcggcc gttcgccacg actttccggt caacatgctg tgctgggcca acctggacgc
    15601 gggcacggcg ctcatcgatc gccatcccga cacgcgattc atcatcgacc atctgggcat
    15661 cctgcagccg cgcgtgccgc ccgcaccgcc gcagccctgg gccgaccttg cgaaggtgct
    15721 ggaactcgcc aagcgtccga acgcggtgat caaggtcagc ggcgcctgca cgctgtcgcg
    15781 ggagccgtat cctttcccgg acatttggga tccgcttgcg cgcgtgtttg atgcgtgggg
    15841 tttcgatcgc tgcttgtggg gcacggattg gacgcgcgcg ttcgccgtcg tcaactacga
    15901 gcaggccgtc gagcccttcc tgaagaccag ccgcctgagc gacaccgagc gggccatgct
    15961 gatgggcggc gcctgcgcca aggcttatgg ctggtcaccg aaaaaaggtt agcaccggct
    16021 gatcatgcgc aacaaggcgg acatccatca acgcggtgga attatgagtt tcacgcgctg
    16081 gccgcggatc agtcgacact gacgccgctg gtcttgatcg gcaccgccca tttggcgatc
    16141 tcactctgca cgaacttcgc gagatattca ggcgtggtgc gatcgtcgga gacgaatttg
    16201 agcccgaagc tttccagctt ctcccgcacc gccggtgttt tcatcgcctc gatcgtcgca
    16261 tggttgagct tttgaacgat ggcgtccggc gtccccttcg gcaggaaaaa cgcgttccag
    16321 gtgtaggcgt cgacgtcaaa accctgctca acggcggtgg ggacgttcgg gagcaccggc
    16381 gagcgctctt tggccaggat ggcgatggcc ttgaccgcgc cgccgtcgat ctgcggcttt
    16441 gcggtggtga tgatatcgca gagatagtcg acgcggccgc tcatcaggtc ctgcatcgcc
    16501 ggattggcgc cgcggtaggg aacgtggacg atatcaagcc cggtcaccat gttcatcagc
    16561 gcgcaaccga gatggatcgc cgaaccggtg ccggcggaag cgaattgcat cttgtccttg
    16621 ttggccttgg cgtaggcgac aaattccttc atgtccttga cgcgaagatc cttgcgcgca
    16681 accagcacca atggggcttc gacgatcagc gccaccggcg caaaatccgt cgtcgaattg
    16741 taggccggct tcttgaacag cgtctggttc tgggcctggg ttccgaccgt cccttgcagg
    16801 aacgtgtagc cgtcgggggc tgccttcgcg acccggtcgg cgccattcat cccgccggcg
    16861 ccggcgacgt tttcgatgat gatcgtctgc cccaggatct cgcccatgcg ttgagcgaac
    16921 agccgcgcca gcacgtcgtt cggcccgccg gctgcgaacg gattgatcat ggtgagcggc
    16981 cgtgtcggcc attgctgggc cctgacgtcg ggcgttgcag ccagcgaggc gagggtgacg
    17041 agcgcggtga cgactttcca catggtgttc tccggagctt gttcctggtg tttggctcag
    17101 acgaatgaaa tggcttcggc catctcgaga attcgtcgaa tttcgatcag ggcgcgctcg
    17161 aggtccgctt cggtggcggg cgcgcccagg caaagccgga cgccgaggct ttcctgctcc
    17221 atccgctgca gcgtgccggg cggcgccagc gtgatgcccg cttgggctgc ctgggcaatc
    17281 agcgccgcca gcgtgcggcc ggccggcagc ggaagccaga tatgaaaacc cggcgcggcg
    17341 aacgctgccg gcagccactc cttcaggacg cgccggacga tggcgtcgcg aagggcggcc
    17401 ttgtcgcgct tcagccgggc ctgccgggca aggccgccat tggcgatcag cctcgccacc
    17461 acttcagcca tgaccggcac cgccatccag ccggtggcgc gcaacgcatt gatcgagcgg
    17521 tcgcgaaatg cttccggcgc gatcatcgcg gcaatgcgca gccccggggc gaggcatttg
    17581 gcgaaactca cgatataaaa gctgcgttcg gggataagcc gggacaccgg cgcgagcggc
    17641 ggcgacaaca aatatccgta cgcgtcatct tccagcagat aggcgtcgtg cttgcgaacg
    17701 atctcggcga tggcgtgccg ccgctgcagc ggcatcaccg atcccgtcgg cgtttgcagc
    17761 gtcggcatgc agtagagaac gcgcgcgccg gtttcggcaa acgcacggtc aaggctttca
    17821 ggaaccagcc ccgatgcgtc ggtcgcaacg ccgtgcaggc gataaccgca ttgcgccgag
    17881 agcgcgagca tgcccgaata ggtgtagctt tcggtcagca cggtatcgcc cggcgatgcg
    17941 accatgccga gcgcgatcga cagcgcgtgc tgcccgccat gcgtgataaa gagatggtcg
    18001 gcgtccgcgg tcacgccgag cgaggtgagc cacgtcacca tcgcctcgcg gtgccttcgc
    18061 aagccctggt gcggcaagta cccgagcagg ttggacagct cgccgtcggc gacgatcgcc
    18121 gccagcactg atgcaatggc gtcgtcttcg ccagtatagg gcgggacatt gagcgccaga
    18181 ttgatcgttg cctccgacga gccgagaccg cgcgcctcgg gccgtcgccg ttgcacgaac
    18241 gtcccgcgtc cgacttcgcc cgagatcaga ccgcgctgct cggcctccgc ataggctttt
    18301 gaaatcgtcc cgacactcag ttccagccgc tgcgccatat cgcgctgcgg caacaaccgc
    18361 atgcccggct tgacccggcc tgcggcgatg tcggcttcca gcgccagaac gaaggcgaga
    18421 taacggggcc cgggaagcga agccagggcg ggtacccagt cggtcat
//
LOCUS       DATNOI010000212         6744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_68003,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000212 DATNOI010000000
VERSION     DATNOI010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68003"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..812
                     /locus_tag="VN968_08210"
     CDS             171..812
                     /locus_tag="VN968_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXL29258.1"
                     /translation="MIEAVIWDFGGVFTTSPFEAFARFESERGLPAGIIRRTNANNHL
                     ENAWAKFERAEVDIETFDKLFAVESLALGAEVRGKDVLPLLVGDLRPDMVEAFKRIAV
                     KLKTGCITNNLPANAIGSLGGRSIYVAEVMALFDHVIESAKIGLRKPDPRIYRIMVEA
                     LHVDPNACVYLDDLGVNLKPAREMGMNTIKVVNAPQAIAELEMVTGLVLRSRS"
     gene            complement(821..1000)
                     /locus_tag="VN968_08215"
     CDS             complement(821..1000)
                     /locus_tag="VN968_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HXL29259.1"
                     /translation="MSDEPYKSWQCRTCGYIYDEAAGAPDEGLAPGTRWADIPDDWVC
                     PECGTPKSDFDMVEL"
     gene            complement(1105..3159)
                     /locus_tag="VN968_08220"
     CDS             complement(1105..3159)
                     /locus_tag="VN968_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HXL29260.1"
                     /translation="MQSAWNDRDAEATVARYENLGRDLALRVYTTRLLGQDRRLVLHG
                     GGNTSVKTGVSDLNGDAVDVLCVKGSGWDMGSIEPAGLPAVRLAPLLKLRGREKLSDE
                     EMVRLQRANLIDPAAPNPSVEALLHAFIPYKFIDHTHSTAVLALTDQPDGEALCREVF
                     GRRVGYVPYLMPGFELAKAAAAVFDADPSVEGLILVKHGIFSFGADARQAYERMIALV
                     TLAEARLARQRKPAFTSAKLPACPAPVADVAPIIRGACSLPDDKTEGAWKRFILDFRG
                     NDAVMNFVNGAEVARFGQAGVVTPDHNIRIKNRPLVVAAPADGDLSGFKNSVRDAVGV
                     YGESYKGYFDRNNARVGGIKTMLDPVPRVVLVPGVGLFGLGRSKKDAKIAADLAEAAV
                     ATITDAEAVGRFEPLPESDLFDVEYWSLEQAKLGNAREPPLTGQVAVITGAAGAIGFA
                     TAKAFAAAGAEVALLDADEAAVHLKAKAIGGAALGLRCDVTDATSVKHAFAQVASVFG
                     GVDIAVSNAGAAWQGRIGEVDEAVLRESFELNFYGHQRVAQASVKIMRAQGTGGCLLF
                     NVSKQAVNPGPDFGPYGLPKAATLFLVRQYALDYGSEGIRANAVNADRVRSGLLTEEM
                     IASRSRARGLSEQAYMRGNLLGREVAAEDVAQAFLAQALALKTTADVTTVDGGNIAAV
                     LR"
     gene            complement(3180..4289)
                     /gene="mtnA"
                     /locus_tag="VN968_08225"
     CDS             complement(3180..4289)
                     /gene="mtnA"
                     /locus_tag="VN968_08225"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HXL29261.1"
                     /translation="MKVDGRHFRSIWLEPDGWTVGAIDQRRLPHDFVVARLTDCDAAA
                     DAIRSMLVRGAPLIGATAAYGMALAMRADSSDAALDHACKTLIATRPTAINLKWALDE
                     MRALLRHLPPSERAAAAYSRAGKIAEEDIGINKAIGHHGLALIEAIAASKKPGERVNV
                     LTHCNAGWLATVDWGTATAPIYLAHDHGHAVHVWVDETRPRNQGASLTAWELGHHGVP
                     HTVIPDNTGGHLMQHAMVDLVMVGTDRVTANGDVCNKIGTYLKALAAHDNGVPFYVAL
                     PSPTIDFSVDDGIKEIPIEQRAADEVTMMTGRTAQGRIETVRIVPDGSPVANYGFDVT
                     PARLVTGLITERGVLGASRAALSAAFPERTAAAAE"
     gene            complement(4508..5383)
                     /locus_tag="VN968_08230"
     CDS             complement(4508..5383)
                     /locus_tag="VN968_08230"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HXL29262.1"
                     /translation="MTKAVLGIIGGSGIYDLPGLENAREEAIRSPWGEPSAPVRHGTI
                     AGLPIVFLPRHDKGHRLSPSDINYRANIDVLKRAGVTDLVSLSACGSFKEELPPGAFV
                     LVDQFIDRTHKRESSFFGKGCVAHVSMAHPVSPRLRIHLAAAAEAEGIAIARGGIYVC
                     IEGPQFSSYAESMTYKNLGYSVIGMTNMPEAKLAREAEICYATVAMVTDFDCWHPDHD
                     AVTVQDIIRVLTSNADKAKSLVARLARDFPSKHEPCPIGSDRSLDTALITGPEARDAE
                     LLKKLDAVAGRVLRA"
     gene            complement(5380..6513)
                     /locus_tag="VN968_08235"
     CDS             complement(5380..6513)
                     /locus_tag="VN968_08235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HXL29263.1"
                     /translation="MNSPLVTVGVPVYRGQQELPVTLECLRTQTYPNLDVLISVDAAD
                     QESARACEPFLRRDARFRMQVQPLRLGWAGNTDWTMRERRGEFYIFQQHDDQVSPTYV
                     ADLVEAASRTPNAAICFAEMHTSGLRTMVSRGFQLSGTSIERALAYLETLDCVPFRGL
                     IRGLALASTSGLLLSDFDPFDSCGTEIRFMAQLALWGEFRFVPGPIYYKRLHGANLHL
                     KRQNWSEHQKQLAWASLAAWMIEVVAPAGRDPEERRRLFDVVLARFLVPRAPWKWVRA
                     PARRLASTSARVLGPLRVLLDRLKHSERILNTVAGRWMLYETSDPQQRVALLGLIFDR
                     LKSGGRFYPSAYLQLTWEALEKEANERFIGRLNRGTKQAQEKP"
     gene            complement(6510..>6744)
                     /locus_tag="VN968_08240"
     CDS             complement(6510..>6744)
                     /locus_tag="VN968_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29264.1"
                     /translation="SLRALDPRELKDVTVKGGVIVAWGQTDIYDPASELHRRFEIGVT
                     STGQFHSIDPGKLTMAPYFADICAGRILEGSRH"
BASE COUNT         1245 a         2185 c         2058 g         1256 t
ORIGIN      
        1 acgcccgcct ccgaggcaga cttgcacatc gtcttgaatt atttcaggtg aattgcagac
       61 gggcgcgcga acctcaagca gccaaaattg gacttgccac cccaaggctg gtccgggccc
      121 gcccagcgtg ttagccgttt cccaagcggc gttcacctgg gtaagcattg atgatcgagg
      181 cagtgatctg ggatttcggc ggggtgttta ctacctcgcc tttcgaggcg ttcgcgcgtt
      241 ttgagagcga acgcgggctg cccgccggca tcatccggcg caccaatgcc aacaatcatc
      301 tggaaaacgc ctgggcgaaa ttcgagcgcg ccgaagtgga tatcgagacg ttcgacaagt
      361 tgttcgcagt ggaatcgctg gcgttaggcg cggaagttcg aggcaaggac gtcttgccgc
      421 ttctggtcgg agacctgcgg cctgacatgg tggaagcgtt caagcgcatc gctgtcaaac
      481 tcaagaccgg gtgcatcacc aacaacctcc ccgccaacgc aatcggcagc ctgggcggcc
      541 gatcgatcta tgtcgccgag gtgatggcac tgttcgatca cgtcatcgag tcggcaaaaa
      601 tcggtttaag gaagcctgat ccgcgcatct atcggatcat ggtggaagct cttcacgtcg
      661 atcccaacgc ttgcgtctat ctcgacgatc tcggcgtcaa tctgaagccg gcgcgagaaa
      721 tgggcatgaa caccatcaag gttgtcaatg cgccgcaagc gatcgccgag ctcgagatgg
      781 tgacggggtt ggtgctgcgt tcgcgttcgt gaatttgtag ctacagctcc accatgtcga
      841 agtcgctctt gggcgtgccg cattcaggac aaacccaatc gtctggaatg tcggcccacc
      901 gggtgcccgg agccaggcct tcgtccggtg cgccggccgc ttcgtcatag atgtagccgc
      961 aagtccggca ctgccaggat ttgtagggct cgtcactcat ttcaattctc ggtttcgcat
     1021 ggtccgtggc cgggagctgt cacgagttta gtggggagat ttcgcgaaga ccagagggct
     1081 gcgccgcagt aacttctact gccgtcaccg cagcacagcc gcgatgttgc cgccatcgac
     1141 ggtagtcacg tcggcggtgg tcttcagcgc cagcgcctgg gccaggaagg cctgcgcgac
     1201 atcctctgcc gcgacttcgc ggccgagcag attgccgcgc atataggcct gctcgctgag
     1261 cccgcgggcc ctggagcgcg atgcgatcat ctcttcggtc aggagaccgg aacggacgcg
     1321 gtcggcattg acggcgttgg cgcgaatgcc ttcgctgcca tagtcgagcg cgtattgccg
     1381 caccaggaac agcgtcgccg ccttcggcag gccataggga ccgaagtcgg gcccgggatt
     1441 gaccgcctgt ttggaaacgt tgaacagcag gcagccgccg gtgccctgcg cccgcatgat
     1501 cttgacggag gcctgcgcca cgcgctggtg accgtaaaaa ttcaattcaa agctctctcg
     1561 caataccgct tcatcgacct cgccgattcg gccctgccag gcggcgccgg cattcgacac
     1621 cgcgatgtcg accccgccga agactgaagc cacttgcgca aacgcatgct tgaccgaggt
     1681 tgcatcggtg acgtcgcatc gaagcccaag cgccgcgccg ccgatcgcct ttgcctttag
     1741 gtgcacggca gcctcgtcgg catcgagcag cgcgacttcg gcgccggccg ccgcgaacgc
     1801 ttttgccgtt gcaaagccga tggcgccggc ggcgccggtg atcacggcga cctgcccggt
     1861 aagcggcggc tccctcgcgt tgccgagttt cgcctgctcc agcgaccagt attcgacgtc
     1921 gaacagatcg gactccggca gcggctcgaa gcggccgacc gcctcggcat ccgtgatggt
     1981 ggcaaccgca gcttccgcaa gatcggcggc aattttcgcg tccttcttgc tccggccaag
     2041 cccgaacagc ccgacgcctg ggaccagcac cacccgcggc accggatcga gcatggtctt
     2101 gatgccgccc acgcgcgcat tgttgcggtc aaaatagcct ttgtaggatt cgccatacac
     2161 cccgacggcg tcacggaccg agttcttgaa accgctgagg tcgccatccg ctggcgcggc
     2221 taccaccagc ggccggttct tgatgcggat attatgatcc ggcgtcacca cgccggcctg
     2281 gccaaagcgc gcgacttccg cgccattgac gaaattcatc accgcatcgt tgcctcggaa
     2341 gtccagaata aagcgcttcc aggcgccctc ggttttatca tccggcaggc tgcaggcgcc
     2401 gcggataatc ggcgcgacgt ctgccactgg tgccgggcag gccggcagtt ttgcgctggt
     2461 gaaggccggc ttgcgctgcc tcgccaaccg cgcttcggcc agcgtcacca gcgcgatcat
     2521 gcgctcgtaa gcctggcggg cgtcggcgcc gaagctgaaa atgccgtgct tgacgaggat
     2581 caggccttcg acggatggat cggcatcgaa taccgcagcc gcagccttgg ccagttcgaa
     2641 ccccggcatg aggtatggca catagccgac ccgccggcca aaaacctcgc ggcacaatgc
     2701 ttccccgtcg ggctgatcgg tgagcgcaag caccgcggtg gaatgggtgt ggtcgatgaa
     2761 tttgtacggg atgaacgcgt gcagcagcgc ctcgaccgag ggattcggcg ccgcaggatc
     2821 gatcagattg gcacgctgca gccgcaccat ttcctcatcg gaaagttttt cgcgcccgcg
     2881 aagttttagc agcggcgcga gccgcaccgc cggcaggccc gcgggctcga tcgagcccat
     2941 atcccagccg gagcctttga cgcagagaac atcgaccgca tcgccgttga ggtcgctgac
     3001 gcccgtcttc accgaggtat tgccgccgcc gtgcagcacc agccgccgat cctggcccaa
     3061 caggcgcgtg gtatagaccc ggagcgccag atcgcggccg agattctcgt agcgggctac
     3121 cgtcgcttcg gcatcgcgat cgttccacgc gctctgcatc gacctgcttc cttggctagt
     3181 tattcggccg ctgccgctgt gcgttcggga aacgcggcgg atagcgcggc gcgactggcg
     3241 ccgagcacgc cgcgctcggt gatcagtccc gtcaccaacc gcgccggcgt gacgtcgaaa
     3301 ccgtaattcg cgaccggcga accgtcgggc acgatccgca cggtttcgat ccgcccctgc
     3361 gcggttcgcc ctgtcatcat cgtgacttcg tcggccgcac gttgctcgat tggaatctcc
     3421 ttgatgccgt catcgacgct gaaatcgatg gtcggcgaag gcagcgccac atagaacggc
     3481 acgccattat catgtgcggc gagggccttg aggtaagtgc cgatcttgtt gcagacgtcg
     3541 ccattggcgg tcacccgatc ggtgccgacc atcacgaggt cgaccatcgc gtgctgcatc
     3601 agatgaccgc cggtgttgtc ggggatcacg gtgtgcggca cgccgtgatg gccgagctcc
     3661 caggcagtga gggaagctcc ctgattgcgc gggcgggtct cgtcgaccca aacatggacg
     3721 gcatggccgt ggtcgtgcgc cagatagatc ggggccgtcg cggtgcccca atccaccgtc
     3781 gccagccagc cggcattgca atgggtcagc acgttgacgc gctcgcccgg tttcttgctc
     3841 gcggcaatcg cctcgatcag cgcaaggccg tgatggccga tagccttgtt gatgccgatg
     3901 tcctcttcgg caatcttgcc cgcgcgcgaa taggctgccg cggcccgttc cgacggcggc
     3961 aaatgccgca gcagcgcgcg catctcgtcg agcgcccatt tcaggttaat tgccgtcggc
     4021 cgcgttgcga tcagcgtttt gcaggcatga tcgagcgcgg catccgaact gtcggcccgc
     4081 atcgccagcg ccatcccata ggctgcggtg gcgccgatca gcggcgcgcc gcgcaccagc
     4141 atcgaacgga tggcgtcagc cgcggcatcg cagtccgtca gccgcgccac gacaaagtcg
     4201 tgcggcagcc ggcgctggtc gatcgcgccg acggtccagc cgtcgggctc aagccagata
     4261 ctgcgaaaat gcctgccatc gaccttcatg acatatccct cgagactcta tagcatttcc
     4321 aggccgctaa tttcatcctt cgagacgcat cgcggagcct gtcatcgggc gcgcattcgc
     4381 gcgacccgtt ggcgatgctc ctccggatga ggtcttggac cctcgtggtg aggagcgcgg
     4441 caaccccgcg cgtctcgaac catgaggccc cttgcctcat catgattcga cacctccaaa
     4501 aatccgctca cgcgcgcaaa acccgccccg ccaccgcatc gagcttcttc agcagctccg
     4561 catcgcgcgc ttcgggaccg gtgatcaacg cggtatcgag cgaacggtcg gagccgatcg
     4621 gacacggctc gtgcttgctg ggaaaatccc tggccagccg cgcgaccagg ctcttggcct
     4681 tgtcggcgtt cgacgtcagc acccggatga tgtcctgtac cgtgacggca tcgtggtcgg
     4741 gatgccaaca atcgaaatcg gtcaccatgg caacggttgc gtaacagatt tccgcctcgc
     4801 gggcgagctt ggcctccggc atgttggtca tgccgatcac cgaatagccg agattcttgt
     4861 aggtcatgct ctcggcatag cttgagaatt gcggaccctc gatgcagaca tagatgccgc
     4921 cgcgcgcgat cgcgatcccc tcggcctcgg ccgcggcggc aaggtggatg cgcagccgcg
     4981 gcgacaccgg atgcgccatc gacacatgcg caacgcagcc cttgccgaag aacgagcttt
     5041 cgcgcttgtg ggtgcggtcg ataaactggt cgaccagcac aaacgcgccg ggcggcaatt
     5101 cttccttgaa cgaaccgcag gccgacagcg agaccaggtc ggtaaccccc gcgcgcttca
     5161 gcacgtcgat gttggcgcgg taattgatgt cggacggcga cagccggtgt cctttgtcgt
     5221 ggcgcggcag gaacacgatc ggcaggcccg cgatggtgcc gtgccgtacc ggcgccgacg
     5281 gctcgcccca cgggctcctg atcgcctcct cgcgcgcgtt ctcgagcccc ggcaggtcgt
     5341 agatgccgga gcccccgata atgccaagca ccgctttcgt catggttttt cctgcgcctg
     5401 cttcgtaccc cgattcagcc tcccgatgaa gcgctcgttt gcttccttct ccagtgcttc
     5461 ccacgtcaat tgaaggtaag ccgatggata gaatcggcca ccgctcttca gcctatcaaa
     5521 aatcaaaccc agcagcgcga cacgttgctg ggggtcgctg gtttcataaa gcatccagcg
     5581 tccggccacg gtgttcagaa ttcgctcgct atgctttagc cgatcgagca ggacacgaag
     5641 cggaccaagt accctggcag aagtcgacgc cagccggcgg gccggagctc tcacccattt
     5701 ccacggtgca cgcggcacaa gaaagcgagc caggacgacg tcaaacagcc gccgccgttc
     5761 ctccggatcg cgtcctgccg gcgcaaccac ctcgatcatc catgcggcta gcgaggccca
     5821 tgccagctgt ttctgatgct cggaccagtt ttgccgtttc aaatgcaaat tggcgccgtg
     5881 caggcgtttg taatagatcg gacccggcac gaagcgaaat tcgccccaca gagcgagttg
     5941 cgccatgaaa cgaatttctg tgccgcagct atcgaacgga tcgaaatcgc ttagcaacaa
     6001 ccctgatgtg gaagccagtg ccaagccgcg gatcagaccg cgaaacggca cgcagtccag
     6061 ggtttccaga taggcgagag cgcgttcgat cgacgtcccc gatagttgaa acccgcgact
     6121 gaccatggtt cgaagaccgc tggtatgcat ctccgcaaag cagatcgccg cgttgggcgt
     6181 gcgcgatgca gcctcgacca ggtcggcgac ataggtcggc gatacctgat cgtcatgctg
     6241 ctgaaaaata tagaattccc cgcgtcgttc tcgcatggtc caatcggtgt tcccggccca
     6301 accgaggcgc aagggctgga cctgcatcct gaaccgggcg tcccggcgca ggaacggttc
     6361 gcacgctctt gcgctctcct ggtctgcggc gtcgaccgag atcagcacgt ccagattcgg
     6421 ataggtctgg gttctcagac actccagtgt aaccgggagt tcctgctgtc ctcgatagac
     6481 gggcacaccg acggtcacca gcggactatt caatgcctgc taccttccag gatgcggccc
     6541 gcgcaaatat ccgcgaaata gggtgccatc gtgagtttgc ccggatcgat ggagtgaaat
     6601 tggcccgtcg aagtgacgcc gatttcgaag cggcggtgca attcgctggc cggatcataa
     6661 atgtcggtct ggccccatgc gacgatgacg ccgcccttga ccgtgacgtc cttcaactcg
     6721 cgcggatcga gagcacgaag cgac
//
LOCUS       DATNOI010000213         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_758749,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000213 DATNOI010000000
VERSION     DATNOI010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758749"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VN968_08245"
     CDS             complement(<1..521)
                     /locus_tag="VN968_08245"
                     /inference="COORDINATES: protein motif:HMM:NF037542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HXL29265.1"
                     /translation="MTSNVVETRLPRSNTSRLSNEAERPTAAADQFSRAKQLAESLLS
                     ERGEASGALLARELHGVLRGLNAGDRHGFQRYLATKFQPDTAALRVAAERYLADTAAE
                     AAAALAQAADPPRQELLRRMNMAPGGTGTLIAMRSEITAHLRHEPELKLLDADLKHLF
                     ASWFNRGFLELRR"
     gene            complement(556..2130)
                     /gene="mdcA"
                     /locus_tag="VN968_08250"
     CDS             complement(556..2130)
                     /gene="mdcA"
                     /locus_tag="VN968_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HXL29266.1"
                     /translation="MCGPGSKICPPERATELLEAIIEPGDRVVIEGDNQKQADFLAAA
                     LAKADPARLHDLHMVQSVLALPDHLAVFERSIANRLDFAFAGPQSRRLAELVASGSVK
                     IGAIHTYLELYARMLVDLTPRVALVVADKADRHGNLYTGPNTEDTPMIVEAAAFSGGV
                     VVAQVNEIVEHLPRVDVPGDWVDVVVQSATHYAIEPLFTRDPAKIRENKILMAMMAIA
                     AVYEPYEIRRLNHGIGYATAAIELILLTFGAARGLKGKLHFVLNPHPTLIPAIEAGFV
                     ESVYCFGSEVGMERYVSARADVFPVGADGNLRSNRALAQAAGQYACDLFVGATLQIDA
                     QGNSSTATKGRIAGFGGAPNMGADARGRRHSSPAWLHAGHQAGDSTRGRKLILQMVET
                     RQPDGMPSFVERLDAIDLATAAGFELPPVMIYGDDVTHVITEQGVANLLLCRSPKERE
                     GALRAIAGDTDFGRAQARDMTDNLRERRIVMQPSDLGIDAKDATRDLLSARTIDDLVV
                     WSGGLYVPPLKFRTSR"
BASE COUNT          382 a          659 c          680 g          412 t
ORIGIN      
        1 atccgccgca gctcgagaaa gccgcggttg aaccaggatg cgaacagatg cttgaggtcg
       61 gcatcgagca gcttcagttc cggctcgtgg cgcaaatgcg cggtgatctc gctgcgcatc
      121 gcgatcagtg tgccggtgcc accgggcgcc atgttcatcc gccgcagcag ttcctgccgg
      181 ggcggatcgg cggcctgcgc gagggcggcc gcggcttcag ccgcggtatc ggcgaggtag
      241 cgttccgccg cgacacgcag tgccgctgtg tcgggctgaa acttggtcgc cagatagcgc
      301 tggaagccgt gccggtcgcc cgcattgagg ccgcgcagta ccccgtgcag ctcgcgcgcc
      361 agcagcgcgc cggatgcttc gccgcgttcg gagagcagtg attccgcaag ctgcttggcg
      421 cggctgaact gatcagccgc ggcagttggc cgctcagctt cgttgctgag acgtgaggta
      481 ttggagcgcg ggaggcgcgt ttcgacaacg ttcgatgtca tctcatcttc cttgttgctt
      541 gagtgatcga ggccgctacc gggacgtgcg gaatttgagt ggcggcacat acaggccgcc
      601 ggaccagaca acgagatcgt caattgtccg cgcggagagc agatcgcgcg tcgcgtcctt
      661 tgcatcgatc ccaaggtcgg acggttgcat cacgatccgg cgttcgcgca ggttgtcggt
      721 catgtctcga gcttgagcgc gtccaaagtc cgtgtctccg gcgatggcgc gcagcgcgcc
      781 ttcacgttcc ttgggagacc ggcacagcag gagatttgcg acaccctgtt cggtgatgac
      841 gtgggtgacg tcgtcgccgt agatcatgac cggcggcaat tcgaatcctg ctgctgttgc
      901 gagatcgatc gcgtcgagcc gctccacgaa gctcggcatc ccatctggct ggcgggtctc
      961 gaccatctgc aggatgagct tgcggcctct ggtgctgtcg cctgcctggt ggcccgcgtg
     1021 cagccaggct ggactggaat gccggcgccc tcgcgcatct gcgcccatgt tgggcgcgcc
     1081 gccgaaaccg gcgatccgtc ccttggttgc cgtcgaactg ttgccctgcg catcaatctg
     1141 aagggtcgcg ccgacgaaga ggtcgcacgc atattggccg gccgcctgcg ccaacgcgcg
     1201 gttggatcgc agattaccgt ctgcgccgac gggaaatacg tcggcgcgtg cggaaacata
     1261 gcgctccatc cccacctcag atccgaaaca atagacactc tcgacaaagc cggcctcaat
     1321 cgccgggatc agcgtcgggt gcggattgag aacgaagtgc aatttgccct tcaggccgcg
     1381 cgcggcgcca aaggtcaaga ggatcaattc gatcgcagcg gtcgcatagc caatgccgtg
     1441 attcagccga cgaatttcat agggttcgta gactgccgcg atggccatca ttgccatcaa
     1501 tattttgttt tcccgaatct tggcaggatc gcgggtgaat aacggttcga tcgcataatg
     1561 cgtcgcgctc tggacgacga catccaccca gtccccggga acatcgaccc gtggcagatg
     1621 ttcaacgatc tcattgacct gcgccacgac cacgccgccg ctgaaagcag cggcttccac
     1681 gatcatgggc gtgtcttcgg tgttcgggcc ggtataaagg ttgccgtgcc ggtcggcttt
     1741 gtccgcgacg acgagcgcga cccgcggcgt cagatccacc agcattcggg cgtagagttc
     1801 aagataggtg tggatcgcac caatcttcac cgagccggac gcaaccagtt ccgccagcct
     1861 ccggctttgc ggcccagcaa aggcaaaatc gagtcggttc gcaatgctcc gctcgaagac
     1921 ggcaagatga tcggggagcg ccagcaccga ctgcaccata tgcagatcat gcaagcgcgc
     1981 agggtcggct ttcgcgagcg ctgccgccag gaaatcggct tgtttttggt tgtcaccttc
     2041 gatcacgacc cgatcgcccg gctcgatgat cgcctcgagc aattcggtgg ctcgctcagg
     2101 tggacagatt tttgagccgg ggccgcacat caa
//
LOCUS       DATNOI010000214         7730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2179648,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000214 DATNOI010000000
VERSION     DATNOI010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179648"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VN968_08255"
     CDS             complement(<1..299)
                     /locus_tag="VN968_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007630249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXL29267.1"
                     /translation="MKIVVIGGTGLIGSKTVAILRQGGHEVVAASPKSGINTITGEGL
                     KAAMAGAQVVIDLANSPSFEDKAVLEFFETSGRNLLAAEAAAGVRHHVALSIVGT"
     gene            complement(375..773)
                     /locus_tag="VN968_08260"
     CDS             complement(375..773)
                     /locus_tag="VN968_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29268.1"
                     /translation="MLGNIGPEMGRKRIAAAAFIQFEFFDLSRTIVRAGMAFCCPAKS
                     EEMAMRASSLFFAGLAVAIISVAPANAEKNTKVEDTYKCSTTTWPVISAFQCNKPVAT
                     SYVECVKMVTDKGWRSSDAWWGCSNQGFKN"
     gene            complement(940..2109)
                     /locus_tag="VN968_08265"
     CDS             complement(940..2109)
                     /locus_tag="VN968_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HXL29269.1"
                     /translation="MTASIVGWAHTPFGKFDTETVESLVVKVATDALADAGISAADVD
                     EIVLGHFNAGFSPQDFTASLVLQADPQLRFKPATRVENACATGSAAVHQGIRAIAAGA
                     AKIVLVVGVEQMTRAPAAEIGRNLLRASYLPEDGDTPGGFAGVFGKIAQGYFQKYGDQ
                     SDALAMIAAKNHKNGVANPYAQMRKDYGYEFCRSESEKNPFVARPLKRTDCSLVSDGA
                     AALVLTDAETAKTMGKAVNIRATAHAQDFLPMSKRDILLFEGCTVAWQRALQSAGVQL
                     SDLSFVETHDCFTVAELIEYEAMGLTPRGQGARAIKEGWTQKDGKLPVNPSGGLKAKG
                     HPIGATGVSMHVMSAMQLAGQAPEGMQIKNAKLAGIFNMGGAAVANYVSVLEPAK"
     gene            complement(2162..2806)
                     /gene="eda"
                     /locus_tag="VN968_08270"
     CDS             complement(2162..2806)
                     /gene="eda"
                     /locus_tag="VN968_08270"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HXL29270.1"
                     /translation="MTAKQEKLAALFRQAVVIPVLTIERLEDAVPLAKALVAGGVRVL
                     EVTLRTPVAIPAAQAIISSVPEAVVGIGTILNADDLARAEALGAVFGISPGATPDLLK
                     AAAASALPFAPGIATASELMQALAHGFNLVKFFPAEQSGGIKALRALAGPFPDARFCP
                     TGGIGEANAAAWLAEPNVSAVGGSWPCPAADIRSGNWTGITAMCNRTMKLLKPA"
     gene            complement(2803..3747)
                     /locus_tag="VN968_08275"
     CDS             complement(2803..3747)
                     /locus_tag="VN968_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HXL29271.1"
                     /translation="MTRVACIGECMIELKQAKGGLYSLGYGGDTLNTSVYLARLGVDV
                     DYVTALGDDTLSDEMIAGWTAEGVGTKRVARLPGKLPGIYMIRTDDKGERRFFHWRES
                     AAARALMDLPETEDILNSLASYDVVYLSAITLSIYSENGRKRLMAALKRARLLGTRFA
                     FDTNFRARGWPDLNMARSVFSDAFAAADIVLASTEDLLPLYPGEANATLMAGISSPEV
                     VLKLSEPASVLRFAGGSHEVKAEPLTKPAVDTTAAGDSFAAAYIAARLAGSDPIDAAR
                     AGHRLAGVVVGYPGAIIPRYAMPARKTPRSASSRKVSR"
     gene            complement(3781..4767)
                     /gene="denD"
                     /locus_tag="VN968_08280"
     CDS             complement(3781..4767)
                     /gene="denD"
                     /locus_tag="VN968_08280"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HXL29272.1"
                     /translation="MHILVLGAAGMVGRKLTERLLRDGRLGDREISRMTLHDVVAPTK
                     PANAAIPISVVTSDFADPDTAASLVGHRPDVIFHLAAIVSGEAELDFDKGYRINLDGT
                     RMLLDAIRLAGGGYKPRAVFTSSIAVFGAPFPEAIGDEFFQTPLLSYGTQKAICELLL
                     ADYSRRGFMDGIGIRLPTICIRPGLPNKAASGFFSNILREPLAGKEAVLPVSEDVRHW
                     HASPRSAVGFLIHAATMDSATAGARRNLTMPGLSATVGEEIAALKRVAGDKVAARIKR
                     EPDPFIMSIVDGWPRNFDARRARQLGFTTAEQTFDDIIKIHIEDELGGKFVA"
     gene            complement(4878..5816)
                     /locus_tag="VN968_08285"
     CDS             complement(4878..5816)
                     /locus_tag="VN968_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL29273.1"
                     /translation="MTDLPAPKPVNLKAVLSGSSPTAEKDDHSEGMSYLESVPRRLVT
                     LYLPLSIIVIILLFPFYWMGLTAIKPDEQLLDLDKFNPFWTWSPTFKHIYKLLFQSYY
                     PHWLWNTMYVAVCATVLSIFASVLAAYAIVRLRYRGAHWVGGLIFLSYLVPPSILFIP
                     LATVVFQYGLFDSPLALILTYPTILIPFSTWLLMGYFKTIPFELEECALIDGASRWQI
                     LIKIVLPLAIPGLISAFIFCFTLCWNEFIYALTFLQSTQNKTVPVAIVNEFVDGDIYR
                     WGSLMAGALAGSLPLVILYAFFVEHYVSAMTGAVKE"
     gene            complement(5809..6768)
                     /locus_tag="VN968_08290"
     CDS             complement(5809..6768)
                     /locus_tag="VN968_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL29274.1"
                     /translation="MADIALQPRVAKRQIREATAWDQLRTNRNWLGFWFMLPAMAFLI
                     FFLAYPLGLGIWMSFTDAKIGRPGEFIGTENYEWLWGDSIFWLSVFNTLLYTTIASAI
                     KFAVGLYLALLLNENMPFKAMLRALVLIPFIVPTVLSALAFWWIFDSQFSIISWSLKH
                     LGLITQNINFLGDVTWARICVIFVNIWRGVPFVAITLLAGLQTVQPSLYEAATLDGAS
                     KWEMFRFITYPLLTPIIAVVMTFSVLFTFTDFQLIWAMTRGGPVNATHLMATLSYQRA
                     IIAGQLGEGAAISSAMIPFLLAAIMISWFGLQRRKWQQGESND"
     gene            complement(6873..>7730)
                     /locus_tag="VN968_08295"
     CDS             complement(6873..>7730)
                     /locus_tag="VN968_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL29275.1"
                     /translation="PVATIGGYMTYRKSATDKAGFSEFPKDFPGFLEMCKALKKNNTP
                     AGFALGHATGDANGWLHWVLWGHGAYTVDKDDKVVINSPETVKALEYCKALSDSFIPG
                     VASWNDSSNNKAFLSGELYCTSNGISIYVAAKDDPTKKDLAEDTYHALYPVGPVGKPT
                     ELQLAVPILAFNFTKYPNASKAFIAFMVEKENYEKWLSGARGYLTHTLNAYDSAPVWT
                     ADPKNQVFSQASKRALPASGIGTPGEKAATAIADFLVVDMFANYCTGTKDAKSAISDC
                     ERQLKRIYR"
BASE COUNT         1501 a         2389 c         2424 g         1416 t
ORIGIN      
        1 tcggttccga cgatggatag cgcgacatgg tgccggacgc ccgctgcggc ctccgccgcg
       61 agaaggttgc ggccggaggt ctcgaagaat tccagcaccg ccttgtcttc aaatgagggc
      121 gaattggcga ggtcgatcac cacctgcgcg ccggccatgg ccgctttgag cccctcgccg
      181 gtgatggtgt tgatgccgct tttgggcgag gcggcgacga cctcgtggcc gccctggcgc
      241 agaatggcga cggtcttcga gccgatcagg ccggtgccgc cgatcacgac gatcttcatg
      301 gttgaccctc ccttgtcgat gactggagat ccctagatga ctggcgatcc taaaatgggg
      361 ttcgaaaaga ttggtcagtt cttgaacccc tgattgctgc acccccacca ggcgtcggag
      421 gatcgccaac ccttgtccgt caccatcttc acacattcga cgtagctggt ggctacaggc
      481 ttgttgcact gaaaagccga aataaccggc catgtcgtag tggagcattt gtaggtatct
      541 tcgacctttg tgttcttttc ggcattcgcc ggtgcaactg agatgatcgc gacggctagt
      601 ccagcgaaga aaagtgacga ggctctcatg gccatttcct ccgactttgc aggacagcaa
      661 aatgccatcc cggcgcggac gatcgtgcgg ctgagatcaa aaaactcaaa ttgaataaat
      721 gctgcggcag ctatgcgctt ccggcccatc tccgggccaa tattgccgag cacggtccta
      781 cgtgcgcgaa gctaaaccaa gacgtcgtcc cagcgaaaag aagggaccca cagccaccgg
      841 cgctagttgt cggtctctgc cgccggtgcc ctattgatgg gacaacgcag tatgggctcc
      901 ggccttcgcc ggggcgacaa tggagttttt tccgagcggc tacttcgcag gctccagcac
      961 cgagacgtaa ttggcaaccg ccgcgccgcc catgttgaag atgcccgcga gttttgcatt
     1021 tttgatctgc atgccctcgg gcgcttgccc tgcgagctgc atggcgctca tcacatgcat
     1081 ggaaacgcca gtggcgccga tcgggtggcc cttggccttc aatccgccgg agggattgac
     1141 cggcagcttg ccgtccttct gggtccagcc ttccttgatg gcgcgggcgc cctgccccct
     1201 cggcgtcagt cccatcgcct catattcgat cagctcggcg acggtgaagc agtcgtgggt
     1261 ttcgacaaag gagagatccg aaagctgcac gccggcggat tgcagcgcgc gctgccaggc
     1321 cacggtgcag ccttcaaaca gcagaatgtc gcgcttcgac atcggcagga aatcctgggc
     1381 gtgcgcggtg gccctgatgt tgacggcctt gcccatggtt ttcgcggttt cggcatcggt
     1441 cagcaccagc gccgcggcgc cgtccgacac cagcgagcaa tcggtgcgct tcaagggtct
     1501 tgccacgaac gggttcttct cgctttcgga gcggcagaat tcgtagccat aatccttgcg
     1561 catctgcgcg tagggattgg cgacgccatt tttgtggttc ttggcggcga tcatcgccag
     1621 cgcgtcggac tggtcgccgt atttctggaa atagccttgt gcgatcttgc cgaacacgcc
     1681 ggcgaaaccg ccgggggtgt cgccatcctc cggcagatag gaggcgcgca gcaggttgcg
     1741 gccgatctcg gctgcgggcg cgcgcgtcat ctgctcgacg ccgaccacca gcacgatctt
     1801 cgcagcaccc gcggcaatgg ccctgatacc ctgatgcacc gccgcggagc cggtggcgca
     1861 ggcattctcg acccgtgtgg ccggcttgaa gcgcagctgc ggatcagcct gcagcaccaa
     1921 tgaggcggtg aaatcctgcg gcgagaaccc ggcattgaaa tgcccgagca cgatttcatc
     1981 gacatccgcg gcggagatac cggcgtccgc cagcgcgtcg gtggcgacct tcaccaccag
     2041 gctctcgacc gtctcggtat cgaacttgcc gaacggcgtg tgcgcccaac cgacgatgct
     2101 ggctgtcatg gtgtgctccc tagtctggct cgcagggttg taacctatcg gccgatttac
     2161 ttcacgccgg tttcagcaat ttcatggtcc gattgcacat ggcggttatg ccagtccaat
     2221 tgccggacct gatatccgcc gccgggcatg gccaggaccc gcccacggct gatacgttcg
     2281 gctcggccag ccacgcggca gcgttggcct cgccgatgcc gccggtcgga caaaaccgcg
     2341 catccggaaa cggcccggcc agggcccgaa gcgctttgat accgccggac tgctcggccg
     2401 ggaagaattt cacaagattg aaaccgtggg ccagcgcctg catcagttcg gacgccgtcg
     2461 cgatgcccgg cgcgaacggc aacgcgcttg cggcggcagc cttgagcagg tcgggtgtgg
     2521 cgccgggact gatgccgaag acggcgccga gcgcttccgc gcgggccaga tcgtccgcgt
     2581 tcaggatggt gccaatcccg acgacggctt ccggcacgct tgagatgatc gcctgtgcgg
     2641 ccggaatcgc aaccggcgta cgcagcgtga cttccagtac ccgcacgccg cccgcaacca
     2701 gtgccttggc gagcgggacc gcgtcttcaa gccgttcgat ggtaagaacc gggatgacga
     2761 cggcctgtct gaacagcgcg gcgagttttt cctgttttgc ggtcatcgtg agaccttgcg
     2821 agaggacgcc gatcgcggtg ttttcctggc cggcatggca tagcgcggaa tgatcgcgcc
     2881 gggatagccc acgaccacgc cggcgagacg atgcccggcg cgcgcggcgt cgatcgggtc
     2941 cgagcccgcc agccgagcgg cgatgtaggc cgctgcaaaa ctgtcgcccg ccgcggtggt
     3001 gtcgacggca ggttttgtca ggggctcggc cttcacctcg tgcgaaccgc cggcgaaccg
     3061 caggacactg gcgggctcgc taagcttcaa caccacttcc ggactggaaa taccggccat
     3121 cagggtcgcg ttggcttcgc cgggatagag cggcagtaaa tcttccgtcg aagccagcac
     3181 gatatccgcg gccgcaaagg catcgctaaa gacgctccgc gccatgttga gatcgggcca
     3241 gccgcgggcg cggaaattgg tatcgaacgc gaagcgggtc ccgagcagcc gcgcgcgctt
     3301 gagagccgcc atcaggcgct ttcggccgtt ctcgctgtag atcgacagcg tgatcgcgga
     3361 gagatagacg acgtcgtagc ttgcgagcga attcaggatg tcctcggttt cgggtaagtc
     3421 catcagggcg cgcgcagctg cactttcgcg ccagtggaag aaccgccgct cacccttgtc
     3481 gtcggtccgg atcatgtaga tgcccggcaa tttgcccggc agccgcgcga cgcgcttggt
     3541 cccgacgcct tcggcggtcc agcccgcgat catctcatcg ctcaatgtat cgtcgccgag
     3601 cgccgtgaca taatcgacgt caacgccaag gcgggcgaga tagaccgatg tgttcaacgt
     3661 atcgccgcca tagccgagcg aatacaggcc gcccttggcc tgcttcagct cgatcatgca
     3721 ttcgccgatg cacgctaccc gggtcatgac cgcttccgca cgggtccgct ggaacgcacc
     3781 ctaggccaca aacttgccgc cgagttcgtc ctcgatgtga atcttgatga tgtcatcaaa
     3841 ggtctgttcg gcggtggtga agccgagctg tcgcgcccgc ctggcgtcga aattgcgcgg
     3901 ccagccgtcg acaatgctca taatgaacgg gtccggctcg cgcttgatcc gcgccgccac
     3961 cttgtcgccc gccacccgct tcagcgccgc gatctcctcg ccgacggtcg ccgaaagccc
     4021 gggcatggtg aggttgcggc gtgcaccggc ggtcgcggag tccatggtcg ccgcatgaat
     4081 caggaacccg accgccgagc gtggcgaggc atgccagtgc cggacgtcct ccgacaccgg
     4141 cagcaccgct tccttgcccg ccagcggctc gcgcaggatg ttggaaaaga accccgaagc
     4201 cgccttgttg ggcagcccgg gacgaataca aatggtcggc agccggatgc cgatgccgtc
     4261 catgaacccg cggcgcgaat aatccgccag cagcagttcg cagatcgcct tctgggtgcc
     4321 gtagctcaac agcggcgtct gaaagaactc gtcaccgatg gcctcgggaa acggcgcgcc
     4381 gaacaccgca atcgaggaag tgaagacggc gcgcggcttg tagccgccgc cggcaagccg
     4441 gatcgcgtcc agcagcatcc gcgtgccgtc cagattgatg cgatagccct tgtcgaaatc
     4501 aagctcagcc tcgccggaga cgatcgcggc gagatggaag atcacatcgg ggcggtgccc
     4561 caccagcgat gccgcggtat cgggatcggc gaagtcgctt gtgacaacgc tgatggggat
     4621 tgccgcattc gcaggcttgg tcggagccac gacatcgtgc agcgtcatcc gcgaaatctc
     4681 gcggtcgccg agacgtccgt cgcgcagcag ccgctcggtc agttttcgcc cgaccatgcc
     4741 ggcggcgccg agaaccagaa tatgcaagac ccaactcctt gttcttgttc tatagcgttt
     4801 tcgagcaaag tgggaaccgg ttcgcgtcaa gaaaacgcgt taaaataaat gagagatgag
     4861 acgcgggccc tgcgcgccta ttccttgacg gctccggtca ttgccgacac gtagtgctcg
     4921 acgaagaacg catagaggat gaccagcggc agcgagcccg ccagtgcgcc cgccatcagc
     4981 gatccccagc ggtagatgtc gccatcgacg aattcgttga cgatggcgac cggcaccgtc
     5041 ttgttctggg tcgattgcag gaaggtcagc gcgtagatga attcgttcca gcacagggtg
     5101 aagcagaaaa tgaacgccga gatcaagccg ggtatggcca gcggcagcac gatcttgatc
     5161 agtatctgcc agcggctcgc cccgtcgatc agcgcgcatt cctccagctc gaacgggatg
     5221 gtcttgaaat atcccatcaa gagccaggtc gagaacggaa tcaggatggt cgggtaggtc
     5281 aggatcagcg ccagcggcga gtcgaacagc ccgtactgga acacgacggt ggcgagcggg
     5341 atgaacagga tcgacggcgg caccagatag gacaggaaga tcagcccgcc gacccaatgc
     5401 gcgccgcggt agcgcagccg cacgatggca taggccgcca gcacgctggc gaagatcgac
     5461 agcacggtgg cgcagaccgc cacatacatc gtgttccaga gccaatgcgg ataatagctc
     5521 tgaaacagca atttgtagat gtgcttgaag gtcggactcc aggtccagaa cggattgaac
     5581 ttgtcgagat cgagcagctg ctcgtccggt ttgatcgccg tcaggcccat ccagtagaac
     5641 ggaaacagca ggatgatgac gatgatcgac agcggcagat agagcgtgac gaggcggcgc
     5701 ggcaccgact ccagatagct catgccctcg ctgtgatcgt ccttctcggc tgtcggcgac
     5761 gagcccgaca gtacggcctt gagattgacg ggcttgggtg cgggaagatc agtcattgga
     5821 ttccccttgc tgccacttgc ggcgctgcag cccgaaccag gaaatcatga tcgcggcgag
     5881 caggaacggg atcatcgcgc tcgagatcgc ggccccctcg ccgagttggc cggcaatgat
     5941 ggcgcgctga tacgacaagg tcgccatcag atgggtggcg ttgaccggtc cgccacgggt
     6001 catcgcccag atcagctgga agtcggtgaa ggtgaacagc accgaaaacg tcatcacgac
     6061 cgcgatgatc ggggtcagca gcggataggt gatgaagcgg aacatctccc acttgctcgc
     6121 gccgtccagc gtcgccgcct cgtagagcga gggctgcacg gtctgcaacc ccgccagcag
     6181 cgtgatcgcg acaaagggca cgccgcgcca gatattgacg aagatcacgc agatccgcgc
     6241 ccaggtgacg tcgccgagaa aattgatgtt ctgggtgatc aggccgaggt gcttgagcga
     6301 ccatgaaatg atcgagaatt gcgaatcgaa gatccaccag aatgccagcg ccgacagcac
     6361 cgtcggcacg atgaagggga tcagcaccag cgcgcgcagc atcgccttga acggcatgtt
     6421 ctcgttcaac aacagcgcca gataaagccc gaccgcgaat ttgatcgcgc tcgcgatcgt
     6481 ggtgtagagc agcgtattga acaccgacag ccagaagatc gaatcgcccc acagccactc
     6541 gtagttctcg gtgccgatga attcgcccgg ccgtccgatc tttgcatcgg tgaacgacat
     6601 ccagatgccg aggccgagcg gataggccag gaaaaatatc aggaacgcca tcgccggcag
     6661 catgaaccag aaaccgagcc agtttcggtt ggtccgaagc tggtcccacg ccgtggcctc
     6721 gcgtatctgc cgcttggcca cccggggttg gagtgcgatg tcagccatgg gcgaaatccc
     6781 gcaatgtttc cggcgaccta gccgtcttgt ccgcgttacg aagtgtactc aatggcgcgg
     6841 gcggcgatag cctgccgccc gctccattgt cgtcagcgat aaatccgctt caattgccgc
     6901 tcgcagtccg agatcgcgct cttggcgtcc ttggtgcccg tgcaataatt ggcgaacatg
     6961 tcgaccacca ggaagtcagc gatcgcggtc gcggccttct cgccgggcgt gccgatgccg
     7021 gaagcgggaa gcgcgcgttt gctggcctgg ctgaacacct ggttcttcgg gtcggcggtc
     7081 cacaccggcg ccgagtcata ggcattcagg gtgtgggtga gatagccccg cgcacccgac
     7141 agccacttct cgtagttctc cttctccacc atgaaggcga tgaaggcctt cgaggcgttc
     7201 ggatatttgg tgaaattgaa cgcgaggatc ggaaccgcga gctgcagttc ggttggcttg
     7261 ccgacaggac caaccgggta cagcgcatga taggtatcct cggcaagatc cttcttggtc
     7321 ggatcgtcct tggctgcaac gtaaatcgag ataccgttcg acgtgcagta gagctcgccc
     7381 gacaggaacg ccttgttgtt ggacgagtcg ttccaggagg cgacgccggg aatgaagcta
     7441 tcggacagcg ccttgcaata ttccagcgct ttcacggttt ccggcgaatt gatgacgacc
     7501 ttgtcgtcct tgtcgaccgt ataggcgccg tggccccata gcacccagtg cagccatcca
     7561 ttggcgtcgc cggtggcatg gccgagcgcg aagccggccg gggtgttgtt cttcttgagc
     7621 gccttgcaca tctcaaggaa gcccggaaag tccttcggaa actcgctgaa gcccgccttg
     7681 tctgtcgccg acttgcggta ggtcatgtaa ccgccgatgg tcgcgacggg
//
LOCUS       DATNOI010000215         3099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2381919,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000215 DATNOI010000000
VERSION     DATNOI010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2381919"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="VN968_08300"
     CDS             <1..1882
                     /locus_tag="VN968_08300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL29276.1"
                     /translation="GILRVSDLRELFDCAETLGRVELPPGKRLAILTNGGGIGVLAVD
                     RLVELGGIPAAITPTTREKLDGVLPPTWSGSNPVDIVGDADPARYAAALEVLLADASN
                     DAILVMNVQTAIASADDIAATVTDVVGKYRQQHRGFAKPVLAVWVGADQKIGDLLSGA
                     GIPNYPTEDDAVRGFMHLVQHREVVATLAQVPPAMPSVFIPDIDAARRIVSAALADGR
                     QWLDPVEIKRLLEAYEIAMVETFAAADAEQAVVHASAIFAQGATVVLKIMSRDIVHKS
                     DVGGVVLNLTTPDAVRSATTDILARAKALRPEARIAGVIVQAMMVRPKARELILGLAD
                     DPTFGTVVVFGRGGTAVEIINDKALALPPLDLQLARDLIERTRVSRLLRAYRDVPAVK
                     PDAVALVLVKLAQLAADIPEIRELDINPLLADEAGVLAVDARVAVGSVDRKFRGSGPA
                     NFAVRPYPSQWQRHLEIKDGWRVLARPIRPEDEPVIHEFLRHVTSHDLRLRFFAPMKE
                     FTHEFIARLTQLDYARAMAFVAFDEATNEMVGVVRIHSDSIYETGEYAILLRSDLKGK
                     GLGWALMQLIIEYAKSEGLKTISGDVLKENTVMLEMCRHLGFEVKADPVEHNICDVKL
                     VL"
     gene            complement(2056..2895)
                     /locus_tag="VN968_08305"
     CDS             complement(2056..2895)
                     /locus_tag="VN968_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXL29277.1"
                     /translation="MAFKDILLTLTSYPEPTPVSVLDHAVSIAATFGAHLAAVSCEVH
                     VQVPGHFLAGSIANIPGIIAGEAEKSRKSARDMLAAFDAAAAKAGVLHETFVEKCPTF
                     EVPDLLVDYARLRDLTLVPVPESYDQWYAETVIFGSGRPTLVLPETSRSRPFELETVA
                     VAWDFSRAAARAVSDALPLLERARKVRVVTVTNEKSLDTKHSAEELAKNLARHGIDVM
                     LDKVDANGRPIGEVLETYTVSHQVDVLVMGAYGHARWREFILGGATKSLLSKPPLPIL
                     FSH"
BASE COUNT          574 a          930 c          991 g          604 t
ORIGIN      
        1 gggaattctg cgggtgtcgg atctccgcga gttgttcgac tgcgccgaga cgctggggcg
       61 tgtcgaattg cctccaggaa aacggctggc gatcctgacc aatggcggcg gcataggtgt
      121 cctcgcggtc gatcggctgg tcgaactggg aggaatcccc gctgccatca cgcccaccac
      181 ccgagagaag ctcgacgggg ttttgccgcc aacgtggtcg ggatccaatc cggtcgatat
      241 cgttggtgac gccgaccccg cgcgctatgc ggcggcgctt gaagtgttgc tggccgacgc
      301 cagcaacgat gccatcctgg tgatgaacgt tcagaccgcg atcgcttcgg ccgacgacat
      361 cgccgcgacc gtgaccgatg tggtcggcaa gtaccgccag cagcatcgcg gctttgccaa
      421 gccggtgctc gcggtttggg tcggcgcgga ccagaagatc ggcgatttgc tcagcggcgc
      481 cgggattccc aactacccca ccgaagacga tgcggtgcgc ggcttcatgc atctggtgca
      541 gcaccgcgag gtggtcgcca cgctggcgca ggtgccgcct gccatgccca gcgtgtttat
      601 acccgacatc gatgccgcca ggcggatcgt tagcgcagca cttgccgacg gccggcagtg
      661 gctcgacccg gtcgagatca agcggctgct tgaggcctat gagatcgcga tggtggagac
      721 gtttgcggct gccgacgccg aacaggccgt ggttcacgcc tcggcgatat tcgcgcaagg
      781 tgcgaccgtg gtgctcaaga tcatgtcgcg agacattgtt cacaagtccg acgtcggggg
      841 cgtcgttctc aatctcacca cccccgatgc cgtgcgttca gcgacaaccg acatcctcgc
      901 gcgggcgaag gcgcttcgcc cggaggcgcg gatagccggc gtcatcgtgc aggcgatgat
      961 ggtgcggccg aaggcgcgcg aactgattct aggcctcgcc gacgatccga cctttggcac
     1021 cgtcgttgtg ttcggccgcg gcggcacggc ggtcgagatc atcaacgaca aggcgctggc
     1081 gcttccgccg ctcgatctgc aattggcccg tgacctgatc gagcgcaccc gcgtctcgcg
     1141 gctgctgcgc gcctaccggg acgtgccggc ggtcaagccg gatgccgtcg cgctggttct
     1201 ggtcaagctg gcgcagttgg ccgcggatat ccctgagatc cgcgagctgg acatcaatcc
     1261 gttgctggcg gatgaagccg gagtgttggc agtcgatgcg cgcgttgcgg tcggatcggt
     1321 ggaccgaaag ttcaggggct cgggccctgc caatttcgcg gtgcggccat atccgtcgca
     1381 atggcagcgg catctcgaga tcaaggacgg ctggcgcgtt ttggcacgcc cgattcgccc
     1441 cgaggacgaa ccggtgatcc atgaattttt gcggcacgtc accagccacg atctgcgctt
     1501 gcggttcttc gcaccgatga aggaattcac ccacgagttc atcgcccggc tgacccagct
     1561 cgactatgcc cgcgcgatgg cctttgtcgc gttcgacgaa gccaccaacg agatggtggg
     1621 cgtggttcgg attcactccg attcgatcta cgagaccggc gaatacgcga tcctgctcag
     1681 atccgatctc aagggaaagg ggctgggctg ggcattgatg cagctgatca tcgaatacgc
     1741 gaaatccgaa ggtctcaaaa cgatctcggg ggacgttctg aaggaaaaca cggtcatgct
     1801 ggaaatgtgc cggcacctcg gtttcgaagt gaaggccgat ccagtcgagc acaacatctg
     1861 cgatgtgaaa cttgtgctct agtccgatca tgagaccggc gcggtctcaa cgcgctcgaa
     1921 gtacatggaa tgacgatcac gttttcgcgg cggtatccag accgatccgg cggagcttca
     1981 gttgcagacg gaatgtatcc ttgtcgcgcc catcttaact ctccacggcg cgctggccgc
     2041 caccgcggcg gatagtcagt gcgagaacag gatcgggagc ggcggtttgg acaacaggct
     2101 cttggttgcg ccccccaaaa tgaactcacg ccatctcgcg tgcccgtaag ctcccatcac
     2161 gaggacgtcg acttgatgtg aaacggtata ggtctcgaga acctcgccga tcggcctgcc
     2221 gtttgcgtcg accttgtcga gcatgacgtc gatgccgtga cgcgccaggt tcttggccag
     2281 ttcctcggcg gagtgtttgg tatcgaggct cttttcgttg gtgaccgtaa cgacgcgcac
     2341 ctttctggcc cgttcgagca gggggagtgc atcggaaacc gcccgggccg cggcccggct
     2401 gaaatcccag gcgacggcca cggtttcaag ttcgaacgga cgcgagcgag aggtttccgg
     2461 caagacaagg gttggccggc cagatccgaa gatcaccgtc tcggcgtacc attggtcata
     2521 ggattccggc acgggtacga gcgtcagatc gcgaagccgg gcataatcga ccagcaggtc
     2581 cggaacttcg aaggtcgggc atttttccac gaaggtctcg tgcagtaccc ccgcctttgc
     2641 cgcggctgcg tcgaatgcgg cgagcatgtc cctcgcgctc ttccggctct tttcggcttc
     2701 gcccgcgatg atgccgggaa tgttggcaat cgaaccggca aggaagtgtc cgggcacttg
     2761 tacatgcacc tcgcaggaaa ccgcggcaag gtgagcgccg aaagtggcgg cgattgaaac
     2821 cgcatgatcg agcacagaca ccggagttgg ctcgggataa ctcgtcagtg tcagcagaat
     2881 atccttgaac gccatgcgcc tctccttttc gagacggcaa tctatcgttc cggaaagcat
     2941 ccgcgtttga tccatcgcaa tggactagta ctgactatga gagatcggtg agtcgcttgc
     3001 tgcccatgct tcgagacgct cgcgtcgctc gctcctcagc atgaggttgt tgtgtttcaa
     3061 caagttagac ctcatcctga ggaggccacg aagtggccg
//
LOCUS       DATNOI010000216         8949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1293306,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000216 DATNOI010000000
VERSION     DATNOI010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8949
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293306"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="VN968_08310"
     CDS             complement(<1..108)
                     /locus_tag="VN968_08310"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509139.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HXL29278.1"
                     /translation="MARDKIALIGSGQIGGTLAHLIGLKELGDVVMFDIA"
     gene            complement(244..1428)
                     /gene="zapE"
                     /locus_tag="VN968_08315"
     CDS             complement(244..1428)
                     /gene="zapE"
                     /locus_tag="VN968_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HXL29279.1"
                     /translation="MLSTPPASFHAHYQALVSSGAIEPDAAQAEAAEAFAGLEQRLAS
                     YKPLRKQGLLGRLFADKDEAPPRGLYVHGDVGRGKTMLMDMFFQQSLVVHKRRAHFHE
                     FMAEVHERIYGFRQNIARGEIADGDVIALTAASIFEQAWLLCFDEFHVTDIADAMILG
                     RLFSKLFELGTVVVATSNVAPEDLYKGGLNRSLFVPFIAQIADHMDVLRLDARTDFRL
                     EKLAGVKMWLVPADRAADAALDKAWDSMTGKAPCKPRDISIKGRVLQVPCSAQGVARF
                     SFADICEKPLAASDYLRLAHDYHTILIDRIPVMDYPERNAAKRFIALIDTLYDNAVKL
                     MASAQADPLSLYLATEGHEANEFKRTSSRLIEMSSESYLALPHGHKDSAASGSSAGLV
                     ET"
     gene            complement(1614..2150)
                     /gene="thpR"
                     /locus_tag="VN968_08320"
     CDS             complement(1614..2150)
                     /gene="thpR"
                     /locus_tag="VN968_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HXL29280.1"
                     /translation="MPRLFTGLEIPAEIGQTLSDLRGGLPGARWIDPENYHVTLRFIG
                     DIDGVAANEIASLLFRINRKPFEVALQGLSSFGGRKPRAVVASIAPSRPLIELQAELE
                     RLMQRFGLDPEGRKFTPHVTLARLRDASNQDVADYLSVRGYFPTQVFTASRFVLFSSR
                     ASTGGGPYVVEDSYALSA"
     gene            complement(2296..3000)
                     /locus_tag="VN968_08325"
     CDS             complement(2296..3000)
                     /locus_tag="VN968_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HXL29281.1"
                     /translation="MARSYGTSVSEVERHGRMFAHMLVLGMALMGASAAFAQAPAVGA
                     GKPIKMVVLGDSLSAGLGLSASAAFPARLKQSLKIKGIDVDMINAGVSGDTSSGGRDR
                     LDWSIPPGTDAVILELGANDALRGTDPKVTRAALTDILARLKARKVAVLLCGMLAPPN
                     YGSDYSARFNAIYPDLAKSFGVPLYPFFLEGVAADARLNQADGMHPTAEGVDVIVKNI
                     LPTVEAFLGAISGQRS"
     gene            2999..3733
                     /locus_tag="VN968_08330"
     CDS             2999..3733
                     /locus_tag="VN968_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL29282.1"
                     /translation="MDSLIKPSSLAGLEPDTISISNVNLSLGTGAARVHILKDISLRV
                     AQGEAIGLIGPSGSGKSTLLMVMAGLERPDSGEVVVNGTPFNALDEDALARFRGRHVG
                     IVFQSFHLIPTMTALENVAVPLELAGNPDASQRAAQELTSVGLGDRLHHYPTQLSGGE
                     QQRVALARALAPDPAILVADEPTGNLDEATGKQIVDLLFTKHAERGMTLVLVTHDTSL
                     AQRCDRVVRLRSGRVDGQLAERETAS"
     gene            3730..6300
                     /locus_tag="VN968_08335"
     CDS             3730..6300
                     /locus_tag="VN968_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXL29283.1"
                     /translation="MTVASEPTHGGRASSLALRYALRELRGGLRGFYVFIGCIALGVM
                     AIAGVGSVAASLGEGLAREGRTLLGGDVTFSLIQREAKPQEVAFLRARGEVSVAATLR
                     GMARAADGRAALVELKAVDAAYPMLGELTLDPKMPVADLLAERDGAFGAAADQTLLAR
                     LDLKIGDRVSIGSATFQIRSAVGAEPDKLAGGLGFGPRFLVSEGGLRATGLLQPGSLV
                     RWIYRVKLPDNAADDRAATALADDARRTLPEAAWEIRSRGNASPQLERTISRFTQFLT
                     LVGLAALLVGGVGVANAVKSHIDRRRDVIAAFKALGATGRDVFTIYLTQVVVLAVAGS
                     VIGLAVGAALPFIIVGVFGKLLPLPVVPALHQGELALSFVYGLLTALAFALWPLGRVH
                     DVPVAALFREAVSSEWHRPRWRYLALMAVVIALLIAVAVGLAYDKRIAAVFVASSVLV
                     FALLRGIAAALMALARRLPRTRMTMLRLAIANIYRPGALTPSVVMSLGLGLAVLVTIT
                     QIDGNLRRQFMAALPDRAPSFYFIDIPTAEAGRFGAFLKETAPQSTVEDVPMLRGRIV
                     SARGVKAEDLKASTDAEWVLQSDRGLTYTGEIPRGSEIVEGKWWGADYDGPPLVSMEK
                     KIADGLRLKIGDEIVVNVLGRDIPATIGNLRNVDWQSLGINFVLVFSPNAFRGAPHTH
                     IATLTENQPDPASDARIIKSVAEAFPTVTSVRVREALETIGNVVTNLVLAIRGASAVT
                     LISAVLVLGGALAAGHRHRVYDAVILKTLGATRARLLGAYALEYLMIGFATAIFGVIA
                     GSVAAWLIVTRLMTLSFIWQAGSAAGVVAAALIVTVGLGLAGTLLALNQKPATVLRNL
                     "
     gene            6586..7377
                     /locus_tag="VN968_08340"
     CDS             6586..7377
                     /locus_tag="VN968_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HXL29284.1"
                     /translation="MSDLDRNYASPFGRAAGRVDAATVDAGLRAYMLRIYNYMSIGLA
                     ITGLAALGVYMAAVTTDPAGAAAKFGNAFLTPFGYAMYVSPLKWLFILAPLAMVFAIS
                     AGIERLKPATSQMLFWVFSALMGISLSSIFLVYTHTSIVRVFFITAATFGALSLYGYT
                     TKRDMSGMGSFLFMGLIGIIIASLVNLFLASSMLQFIVSVVGVLVFAGLTAWDTQRLK
                     NDYIYGYASQGGDVAERAAITGALSLYLNFINLFTLLLQLLGQRD"
     gene            7474..8013
                     /locus_tag="VN968_08345"
     CDS             7474..8013
                     /locus_tag="VN968_08345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL29285.1"
                     /translation="MSSPEIRPATAADLSAITEIYEHAVRYGTATFELIPPDLAEMTR
                     RFGVLMDGGFPYLVATLEGRVVGYAYAGAYRPRPAYRFTVENSVYLEPAIHRRGIGLL
                     LLQRLIIECEARGYRQMIAVIGDTANAGSIGVHTRTGFQMIGTHPSVGFKFGRWLDTV
                     MMQRALGEGATTLPADSKA"
     gene            complement(8431..8802)
                     /locus_tag="VN968_08350"
     CDS             complement(8431..8802)
                     /locus_tag="VN968_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29286.1"
                     /translation="MSNPTTDISRDWLSSKRTNLLAWWIPNSAILAGLFAPVSLRTAV
                     WVISLGWMGAACIMNARRCNRTHCRYTGPYFIAMIVPVMALGAALVTVGILGWICLGV
                     IILGGAGLIWWATERAWGKFS"
     gene            complement(8810..>8949)
                     /locus_tag="VN968_08355"
     CDS             complement(8810..>8949)
                     /locus_tag="VN968_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HXL29287.1"
                     /translation="ECTGCGTKLKPKSGDCCVFCSYGSVPCPPIQAQRAGEPGAAPCC
                     S"
BASE COUNT         1611 a         2899 c         2789 g         1650 t
ORIGIN      
        1 ggcaatgtcg aacatcacga catcgccgag ctccttcaag ccgatgaggt gagccaacgt
       61 tccgccgatc tgaccggagc caatcaaagc aatcttgtcg cgcgccatgg gaaatctgtc
      121 ctttgaaccc gaaggaggag gatggatgga agctggatgg ctggttatcc ctttcgaccg
      181 acccgttcaa gtcgcggcgt gctcaattgt cggtcttgcc ttgatttcga ccagccgcgc
      241 gcgtcacgtt tccaccaatc cggcgctgga gccgctggcc gcggagtcct tgtgcccgtg
      301 aggcagcgcc agataggatt cagagctcat ctcgatcagc cgcgacgagg tccgcttaaa
      361 ctcgttggcc tcgtggccct cggtggcgag atacagcgac agcggatcgg cctgcgccga
      421 agccatcagc ttcacggcgt tgtcgtagag cgtgtcgatc aaggcgatga accgtttggc
      481 ggcgttgcgc tcggggtagt ccatcacggg aatgcggtcg atcaggatgg tgtggtagtc
      541 gtgcgcgagc cgcagatagt ccgatgcggc gagcggcttt tcgcagatat ccgcgaatga
      601 aaatcgcgcg acgccctgcg ccgagcatgg cacctgcagg acacggccct taattgaaat
      661 atcacgcggc ttgcaaggcg ccttgccggt catgctgtcc cacgccttgt cgagcgcggc
      721 atcggcggcg cgatcggccg gcaccagcca cattttcacg cctgccagct tctccagccg
      781 aaaatcggta cgggcatcga gccgcagcac gtccatatga tcggctatct gcgcgatgaa
      841 cggcacgaac agcgaccggt tcagaccgcc cttgtagaga tcctcgggcg cgacgttgga
      901 ggtcgccacc accacggtgc cgagctcaaa cagttttgag aacaaccgtc ccaggatcat
      961 cgcatcggcg atgtcggtga catgaaattc gtcgaaacat aacagccagg cctgctcgaa
     1021 aatcgacgcg gcggtgagcg cgatcacatc gccgtcggcg atctcgccgc gcgcgatgtt
     1081 ctgacgaaag ccatagatcc gctcatgcac ctcggccatg aattcgtgga aatgtgcgcg
     1141 gcgcttgtgc acgaccaggc tttgctgaaa aaacatatcc atcagcatgg tcttgccgcg
     1201 cccgacgtcg ccgtggacat agaggccgcg cggcggcgcc tcgtccttgt ccgcgaacag
     1261 ccggccaagc aggccttgct tacgcaaggg cttgtagctt gcaagccgct gctcgaggcc
     1321 cgcgaaggct tcggcggctt cggcctgcgc ggcgtcgggt tcaatcgcgc cggaggagac
     1381 gagggcctga taatgggcgt ggaacgaggc gggcggtgtg gacagcatgg cccccttacg
     1441 gccctaggcc aacggaaatt gcaagccggg atttgggacg ggggctatgc aggtgcgaga
     1501 attacggacg ctgcggcagc gttgagcaag ccgtcatacc cgccaaccgg tccggccttt
     1561 ggccggccgg atgacggata atgacggctg tgaatagggc gcgtccatgc cttctaagcg
     1621 ctcagcgcgt aggaatcctc gaccacatag ggcccaccac cggtggacgc ccgggacgag
     1681 aacagcacga accgcgatgc cgtgaacacc tgggtcggaa aatatccgcg caccgacaga
     1741 taatcggcga catcctgatt ggaggcgtcg cgcagccgcg ccagcgtcac atgcggggtg
     1801 aatttccgcc cctcgggatc gagcccgaag cgctgcatca gccgctcaag ctcggcctgc
     1861 agctcaatca gcggccggct cggcgcgatc gaggcgacca ccgcgcgggg cttgcgcccg
     1921 ccaaagctcg acagcccctg cagggcaacc tcgaatggct tgcgattgat ccgaaacagc
     1981 agcgaggcga tctcattcgc cgcgacgcca tcgatatcgc cgatgaagcg caaggtgacg
     2041 tgataatttt cgggatcgat ccagcgcgcg cccggcaagc cgccgcgcaa atcggaaagc
     2101 gtctggccga tctcggccgg aatttccagt ccagtgaata atcgcggcat cgcaagactc
     2161 ccgatgctga aacccggtaa cgaatcaccg atagcttttt tctaacgtag ttatgtgact
     2221 ctgcgaagct tccgcagttc aaacacggca aagcatccgg gaaaacaccg cattagggtt
     2281 atcgctgcct gtttttcaac tgcgttgccc ggatatcgcg ccaagaaatg cctccacggt
     2341 aggcagtata ttcttaacga tcacgtcgac gccctccgcc gtcggatgca tgccgtccgc
     2401 ctgattgagc ctggcgtccg cggcgacgcc ctccaggaaa aacggataca atggcacccc
     2461 gaacgatttc gccagatcgg gatagatcgc gttgaaacgc gctgaataat cgctcccata
     2521 attcggcggc gcaagcatcc cgcacagcaa aactgcgacc ttgcgcgctt tcagccgcgc
     2581 cagaatgtcg gtcagcgcgg cccgggtaac cttggggtcg gtgcctcgca gggcatcgtt
     2641 ggcgccgagt tccaggatca cggcgtcggt tcccgggggt atcgaccagt cgagccggtc
     2701 acgaccgccg gagctggtat cgccggagac cccggcgttg atcatatcaa catctatccc
     2761 cttgatcttc aaggattgtt ttagccgcgc cggaaacgcc gcggaggccg aaaggccaag
     2821 tcctgcgctc agcgaatcac ccaatacaac catcttgatc ggcttgcccg ccccgacagc
     2881 cggcgcttgc gcaaatgccg cgctcgcccc catcagcgcc atccccaaca cgagcatgtg
     2941 cgcgaacatc cgcccatgcc tctcgacctc gcttaccgaa gtgccatatg accgagccat
     3001 ggacagtctc atcaaaccct cgtcattggc cggccttgag ccggacacca tttcgatctc
     3061 gaacgtcaac ctctcgctcg gcacgggcgc ggcgcgcgtt cacatcctca aagatatcag
     3121 cctgcgggtg gcacaaggcg aagcgatcgg cttgatcggg ccttcaggtt cggggaaatc
     3181 caccttgctg atggtgatgg cgggattgga gcgtcccgac agcggagagg tggtggtcaa
     3241 cggcacccct ttcaatgccc tcgacgagga cgcgctggcg cgctttcgcg gccgtcacgt
     3301 cggtatcgtg ttccagtcgt ttcacctgat tccgaccatg acggcgctgg aaaacgtcgc
     3361 ggtgccgctg gagctggccg gtaatcccga tgcgtcgcag cgcgcggcgc aggaactgac
     3421 ctcggtcggt ctcggcgacc gcctgcatca ctatccgacc caattgtcgg gcggcgagca
     3481 gcagcgcgtg gcgcttgccc gcgcgctggc acccgatccg gcgatcctgg tggcggacga
     3541 gccgaccgga aatctcgacg aagccaccgg caagcagatc gtcgatctgc tgttcaccaa
     3601 acacgccgaa cgcggcatga ccctggtgct ggtgacccac gacacctcgc tggcgcagcg
     3661 ctgcgatcgc gtggtgcggc tgcgctcggg ccgcgtcgat ggacagcttg cggaacgcga
     3721 aaccgcttca tgaccgttgc ctccgaaccg acccatggcg gccgcgcgtc atcgcttgcc
     3781 ctgcgctacg ccttgcgcga attgcgcggc ggcttgcgtg gcttttacgt gttcatcggc
     3841 tgcatcgcac tcggcgtgat ggcgattgcc ggcgtcggct cggtcgccgc cagcctcggc
     3901 gagggcctgg cgcgcgaggg ccgcaccctg ctcggcggcg atgtgacctt ctccctgatc
     3961 cagcgcgagg ccaaaccgca ggaagtcgcg tttcttcgcg cgcgcggcga ggtatccgtc
     4021 gcggcgacgt tgcgcggcat ggcgcgcgcc gccgacggcc gcgccgcatt ggtcgagctg
     4081 aaggcggtgg atgccgccta tccgatgctg ggcgaattga ccctcgaccc gaaaatgccg
     4141 gtggccgatc tgctggcgga gcgtgacggc gcattcggtg cggctgccga tcagaccttg
     4201 ctggcgcggc tcgatctcaa gatcggcgac cgcgtcagca tcggcagcgc gacctttcaa
     4261 atccgcagcg cggtcggcgc cgagccggac aagctcgccg gcgggctcgg tttcggtccg
     4321 cgattcctgg tcagcgaagg cggcttgcgc gcgaccgggt tgttgcaacc cggcagcctg
     4381 gtgcgctgga tctatcgggt caagctgccg gacaatgcag cggacgatcg tgccgcgacc
     4441 gcgctggccg atgacgcgcg cagaaccttg ccggaagccg cctgggagat ccgcagccgc
     4501 ggcaacgcct cgccgcagct ggagcgcacc atcagccgct tcacgcaatt tctgacgctg
     4561 gtcggccttg cggcgctgct ggtcggcggc gtcggcgtcg ccaatgccgt caaaagccat
     4621 atcgatcgcc gccgcgacgt catcgctgcg ttcaaggcgc tgggcgccac cgggcgcgac
     4681 gtcttcacga tctatctgac gcaggtcgtc gtgctcgccg tggccggctc ggtgatcggg
     4741 ctcgccgtcg gcgcggcact gcccttcatc attgtcggcg tattcggcaa gctgctgccg
     4801 ctgccggtgg tgccggcgct gcaccagggt gaactcgccc tgtcgttcgt ctacggcctg
     4861 ctgaccgcgc tcgccttcgc cctttggccg ctcggcaggg tgcatgatgt gccggtggcg
     4921 gcgctgtttc gcgaagcggt gagcagcgaa tggcatcgcc cgcgctggcg ctacctcgcg
     4981 ctgatggccg tggtgatcgc attgctgatc gcggtggcgg tcgggctcgc ctacgacaag
     5041 cgtatcgcgg ccgtattcgt cgcatcctcg gttctggtgt tcgcgctgtt gcgcggcatc
     5101 gccgccgcct tgatggcgct ggcgcgccgc ctgccccgca cccgcatgac catgctgcgg
     5161 ctggcgatcg ccaatatcta ccggcccggc gcgctgacgc cgtcggtggt gatgtcgctc
     5221 ggcctcggtt tggccgtgct ggtcacgatt acccagatcg acggcaatct gcggcggcaa
     5281 ttcatggccg cactgcccga tcgcgcgccg tcgttctatt tcatcgatat tcccaccgcc
     5341 gaagccggcc gcttcggcgc attcctcaag gaaaccgcac cgcaatcgac cgtcgaagac
     5401 gtgccgatgc tgcgcggacg cattgtctcc gcccgcggcg tcaaggccga agacctcaaa
     5461 gcctcgaccg acgccgaatg ggtgctgcag agcgaccgcg gcctgaccta taccggcgaa
     5521 atcccgaggg gctccgagat cgtcgagggc aaatggtggg gagccgatta tgacggcccg
     5581 ccgctggtct cgatggagaa gaaaatcgcc gacgggctca ggctcaagat aggcgacgag
     5641 atcgtcgtca acgtgctcgg ccgcgatatc ccggccacga tcggcaattt gcgcaacgtc
     5701 gactggcaga gcctcggcat caatttcgtg ctggtgttct cgcccaacgc cttcaggggc
     5761 gcgccccata cccatatcgc gacgctgacc gagaaccagc cggaccccgc cagcgacgcc
     5821 cggatcatca aatcggtggc ggaagcattc ccgacggtca ccagcgtgcg ggtccgcgag
     5881 gcgcttgaaa ccatcggcaa cgtggtcacc aacctggtgc tggcgatccg gggcgccagc
     5941 gcggtcacgc tgatctccgc cgttctggtg ctgggcgggg cgctggccgc cggccaccgc
     6001 catcgggtct acgacgcggt cattctcaag actttgggcg ccacccgggc ccggctgctc
     6061 ggcgcctacg cgctggaata cctgatgatc ggctttgcca ccgccatctt cggcgtcatc
     6121 gccggctcgg tggcggcctg gctgatcgta acccggctga tgaccctgag tttcatctgg
     6181 caggccggca gcgccgcggg cgtggtggcg gcggcgctga tcgttacggt gggactgggg
     6241 ctcgcgggaa ccttgctggc tctgaaccag aagcccgcca cagtattgcg gaatttatga
     6301 caaattgaag caggcgttac tgcagagtta acgcagatgc gcgtaaagcc ccgtatatcg
     6361 gggctttaca gccgcttcga cgtcgcctga tatcatgccg cagagcgaca ttcagccgcg
     6421 cgtggaagct tgcagcaaat gtgttagttt cccacatacc agcctgggac agaagccggt
     6481 ttgatgacac gaatttaatg tcggcaaatg gcggcatctg ggatagatct taatcggcgc
     6541 cgcaaaaccc ttgcgctccg ccggacaacc aacgggaatt cgaccatgtc ggacctagac
     6601 cgtaactacg cttctccttt cggccgggcc gccgggcgcg ttgacgccgc gaccgtcgac
     6661 gccggtctgc gcgcctacat gctgcgcatc tacaactaca tgagcatcgg cctggccatc
     6721 accggcctcg ccgcgcttgg cgtgtatatg gccgcggtga caaccgatcc cgcgggcgct
     6781 gctgccaagt tcggcaacgc cttcctgacg ccgttcggct acgccatgta tgtgagcccg
     6841 ctgaaatggc tgttcatcct ggcgccgctg gcgatggtgt tcgcgatctc ggccggcatc
     6901 gaacggctca agcccgcgac gtcgcagatg ctgttctggg tattctcggc gctgatgggc
     6961 atttcgctgt cgtcgatctt cctggtgtac acccacacct cgatcgtgcg ggtgttcttc
     7021 atcaccgcgg ccacgttcgg cgcgctgagc ctctatggct acaccaccaa gcgtgacatg
     7081 agcgggatgg gatcgttcct gttcatgggc ctgatcggca tcatcatcgc aagcttggtc
     7141 aatctcttcc tcgccagctc gatgctgcag ttcatcgtgt cggtggttgg tgtgctggtg
     7201 ttcgcgggcc tcaccgcctg ggatacccag cggctgaaga acgactacat ctacggctat
     7261 gcctcgcagg gcggtgacgt ggcggagcgc gccgccatta ccggtgcgct gtcgctgtac
     7321 ttgaacttca tcaacctgtt cacgctgctg ctgcagctgc tcggccagcg cgactgaccg
     7381 ctgagccacc gctgagtcct ggctgacaaa ttcgagcccc ggccggaagg ccggggcttt
     7441 tcttttgcct gaagcgaaaa taatctcgac gccatgtcct ctcccgaaat ccggcccgcc
     7501 accgcggccg acctctctgc cattaccgag atctacgaac acgcggtccg ctacggcacc
     7561 gcgacgttcg agctgatccc ccccgatctc gccgagatga cgcggcgctt tggcgtgctg
     7621 atggacggcg gctttcccta ccttgtcgct accctcgaag gccgcgtggt cggatacgcc
     7681 tatgcgggag cgtaccggcc gcggccggcc tatcgcttca cggtggagaa ttcggtttac
     7741 cttgagcccg cgatccaccg ccgcggtatc ggcctgttac tgctgcaacg gctgatcatt
     7801 gagtgcgagg cgcgaggcta tcgccagatg atcgcggtca tcggcgatac cgccaatgcc
     7861 ggctcgatcg gcgtccacac caggaccggc ttccagatga tcggaaccca tcccagcgtc
     7921 ggcttcaaat tcggccgctg gctggacacg gtgatgatgc aacgcgcgct cggcgaaggc
     7981 gccaccacgc tgccggcgga ctcgaaggcc tagaatcgca gggtgtgcaa agcgacttgt
     8041 ccgccgcagc tcgaagagcg acctgtccgc catagcccaa ggggcgacgg cggaaggcgg
     8101 aaagcgtgcc catccttcgc gctaacgatc tcgtgtgtcc cggacgcggc ttcggcggtg
     8161 aaccgccggg cctggacaca aaatcgcgaa aacaacccca tgcaaagtag actgggtcca
     8221 ttcaaagtcg ctgagttcgt agcgcatgat tcgagactcc atgtttcgcg gtgccgctaa
     8281 tgctatgaaa tcacttccgc tttcggcggc atcgcggacg tggccggact tgctgctggc
     8341 tcgacccagt cgcgaatgac ccaatctcgg acatctgggc tccgccacat tcggaggcgc
     8401 aaataagacc cttttggaag ttgtcgtgcg ctacgagaac tttccccatg cccgctcggt
     8461 agcccaccat atgagtccgg ctcctccaag gatgatcacg cccaaacaaa tccatccaag
     8521 tatgccgacg gtgacgagcg ccgccccgag cgccatcacg ggcacgatca tggcgatgaa
     8581 gtagggcccg gtgtatcggc agtgtgtgcg attacagcgt cgcgcattca tgatgcacgc
     8641 cgcgcccatc caaccgaggg agatgaccca gacagcagtt cgcagcgata ccggagcgaa
     8701 caaaccggcg aggatcgctg agtttggtat ccaccacgcg agcaaattgg tgcgtttgct
     8761 gctgagccaa tcgcgcgaga tgtccgttgt cggattgctc atggtggaac taggaacaac
     8821 acggcgcggc gcctggctcg ccggcgcgct gggcctgaat tggcggacac ggcactgaac
     8881 cataggaaca gaacacgcaa caatcgcccg acttcggctt tagcttggtg ccgcagccag
     8941 tacattcat
//
LOCUS       DATNOI010000217         3405 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1988161,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000217 DATNOI010000000
VERSION     DATNOI010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988161"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VN968_08360"
     CDS             complement(<1..289)
                     /locus_tag="VN968_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL29288.1"
                     /translation="MPVKVHSLDHLVINVSDVARSAEWYRKVLGMEVRVFDPGQGKPP
                     RTSLIFGNQKINVRPRDADKVEWFTADHETAGSDDLCFLTSSTPDEVVAHLK"
     gene            427..1402
                     /locus_tag="VN968_08365"
                     /pseudo
     CDS             427..1402
                     /locus_tag="VN968_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
     gene            1476..1730
                     /locus_tag="VN968_08370"
     CDS             1476..1730
                     /locus_tag="VN968_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HXL29289.1"
                     /translation="MRNAMLAMVALMSAGAATIAGPTPAAAIDYPYCIQGGGWGVPGD
                     CSYRSYGECMAAASGRRVYCNINPRVAFGQQRRGRYYRYY"
     gene            1949..2404
                     /locus_tag="VN968_08375"
     CDS             1949..2404
                     /locus_tag="VN968_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXL29290.1"
                     /translation="MVKYSEPMLDRTFAALADPTRRALLARLRQRDGISVSELAQPFA
                     MSLPAIMKHLDVLSDAGLVARAKTGRVVACRLTAGPMEQAMDWLNRYQRFWSDNLDRL
                     AAFVEEEQWPPNQALPATPALSRDRASPSNVVSMPRPRKSTRRGRTRNK"
     gene            2389..2748
                     /locus_tag="VN968_08380"
                     /pseudo
     CDS             2389..2748
                     /locus_tag="VN968_08380"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
     gene            2776..>3405
                     /locus_tag="VN968_08385"
     CDS             2776..>3405
                     /locus_tag="VN968_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXL29291.1"
                     /translation="MQPHKIVSREGWIEARKAHLAHEKEYTRARDRLSEARRALPWVK
                     VEKAYVFDGPKGKQTLADLFAGRSQLVVQHFMFAPDWTEGCKSCSFWADGFDGFFVHL
                     AQRDVAYVAVSRATLAEIEAYKKRMGWTAKWVSSYGSDFNFDYGVSFTKDDETKGTMH
                     YNYGTEPYRGEEVHGLSVFSKKETGDVFHTYSAYARGPEELLGFYFYLDR"
BASE COUNT          662 a         1029 c         1069 g          645 t
ORIGIN      
        1 ccttgaggtg cgcgaccacc tcgtccggcg tgcttgaggt gagaaagcac agatcgtcgc
       61 tgcctgcggt ctcatggtcc gcggtgaacc actcgacctt gtcggcgtcg cgcggccgca
      121 cattgatctt ctggttgccg aagatcagcg acgtccgtgg cggcttgccc tggcccggat
      181 cgaatactct gacctccatg ccgaggacct ttcgatacca ttcggcggag cgcgccacat
      241 cggagacatt gatcacgaga tgatcgagcg aatggacttt gacgggcatg gattatcctt
      301 tggtcgcagt tcgatgcgcg cctgccgcta ctagctgcat caggctttgt ttgttacaac
      361 gcggcccctc gtcaactcaa cagataccaa ccggaccttt tagattcggg acaggagaga
      421 aacggcatgg ttttacgtcg caccgcgatt tgtctgatcg caatcggcat ctccgcaatc
      481 gcctcgatcg gcagcgcctc ggcgctggat tatccgacgc ggccggtccg ctgggtggtc
      541 ggctatcctc caggcggcgc gaccgatatt ctggcgcggt tgatcggcca gcggctctcc
      601 gagcggctcg gccagcaatt cgtgatcgag aacaagcccg gcgccggcaa caacatcgcc
      661 accgaggcgg tggtcaatgc ggagcccgat ggctatacgg tgctgctggt caatcccgcc
      721 aactacatca acgcctcgct ctacggcaat ctcaaattca acttcatccg cgacatcgcg
      781 ccgattgcgg cgttcaatcg cgtgcccaat gtgatgacgg tcaataatga cgtgccggca
      841 aagtcggtcg ccgagttcat tgcctatgtg aaggacaatc cgggcaaggt gaacatggca
      901 tcatcaggca acggcacctc ggtgcatctg tccggtgaaa tgttcatggc gatgtcgggc
      961 accaagatgc agcacgtgcc ctatcggggg ggccgcacct gcgatcaccg acatgcttgg
     1021 cggccaggtt cagttgatct tcgacaacat gccctcgatc atccagcata tccgcgccgg
     1081 ttccttgcgc gcgctggcgg tgacctcgac ggcgcggtcc ccgctcttgc cggatgtccc
     1141 ggccctcgcc gacaccattc ccggctatga ggcgagcgcg ctgttcggca tgggcgtgcc
     1201 gaagaatacg ccgaaggaaa tcatcgagaa gctgaacaag gagatcaacg ctgtgctcgc
     1261 ggaacccgcg atcaaggcga agctgatcga cctcggcggt gagccggtga tcgggccgcc
     1321 ggaagcgttc ggcgcgatga tcgtggccga aaccgaaaaa tgggagaagg tggtcaagtc
     1381 cgccggcgtg cacgtggact gaaacagctg ccggaacctt ctcgaagcgg tatcgttttg
     1441 aagacgaggg cgaactcgcg cggaggagga gaactatgcg caatgcgatg ctggcgatgg
     1501 tggctttgat gtcagccggg gctgccacga tagccggccc tacgcccgcc gccgcgattg
     1561 attatcccta ttgcatccag ggcggtggtt ggggcgttcc gggcgattgc tcctaccggt
     1621 cctatggcga atgcatggcg gcggcctccg gccgacgcgt ctattgcaac atcaatcctc
     1681 gcgttgcatt cggccagcag cgccgcggcc ggtactatcg gtattattga aaccggtctg
     1741 cgagcgtcat ccgttcgtcg tcccggcctt gagccgggac ccatactccg agaacttgcg
     1801 attcaagggc ggtgtacaac gactgcctta acatacgcgc cggtgacgtt cgccgggacg
     1861 actcgttgcg agcttgcgac gtcactccgg tcgtgcccgg caagctggta gacttgacac
     1921 gtctttcgtt gccgctatac taaaccatat ggttaagtat agcgagccga tgctggaccg
     1981 cacctttgcc gcgctcgcgg acccgacgcg gcgggcgctg ctggcgcggc ttcgccaacg
     2041 ggacggcatc tcggtcagcg agctggcgca gccatttgcg atgtcgctgc ccgcgatcat
     2101 gaagcatctc gacgtgctgt cggacgcagg cctggtcgcg cgcgcaaaaa ccggccgcgt
     2161 tgtggcgtgc cgcctgaccg cggggccgat ggagcaggcg atggactggc tcaatcgcta
     2221 ccagcgcttc tggtcggata atctcgaccg ccttgccgct ttcgtggagg aagaacaatg
     2281 gccaccaaat caagcgctgc cggcgacacc ggccttgtca cgcgaccgag cctcaccctc
     2341 aaacgtcgtc tcaatgcctc gcccgagaaa gtctacgcgg cgtggacgaa cccgcaacaa
     2401 atagcccgct ggttcgggcc gtcgtcggtc aaggccggca ccgggcaggc cagcatcgac
     2461 gcgtggatcg gcggacgcta ccgcatcggc ttcaccaacg aagacggaga gcatcatgaa
     2521 gtcggcggcg tctatcgcga gatggcgccg aaccagcggc tggtgttcag ctgggcctgg
     2581 cattcgacgc cggaacgcga atcgctggtg acggtgtcgc taaagcctga cggcgacggc
     2641 acgctgctca ccctccacca cgagcaattg ttcgatcagg ccgcgcgcga cggccatgag
     2701 cgcggttgga tcggcacgct cgacaagctt ggaaagcatc tcgcctgaaa cgtttcaatg
     2761 taagaggagc cggtcatgca accgcacaaa atcgtctcgc gtgaaggatg gatcgaagcc
     2821 cgtaaagcgc atctggcgca tgaaaaggaa tacacaaggg cgcgtgaccg gctgagcgag
     2881 gcgcggcgcg cgctgccctg ggtcaaggtc gagaaggcct atgtgttcga cggcccgaag
     2941 ggcaagcaga cgctggccga tctgttcgcg ggccgcagcc agttggtggt gcagcatttc
     3001 atgttcgcac ccgactggac cgaaggctgc aagagctgct cgttctgggc cgacggcttc
     3061 gacggcttct tcgtgcatct ggcgcagcgc gacgtcgcct atgtcgcggt gtcgcgcgcg
     3121 acgctggcgg agatcgaggc ctacaagaag cggatgggct ggaccgccaa gtgggtgtcg
     3181 tcctatggca gcgacttcaa cttcgattat ggcgtgtcgt ttacgaagga cgatgagaca
     3241 aagggcacga tgcactacaa ctatggaaca gagccatacc gaggcgagga agtgcatggt
     3301 ctcagtgtgt tcagcaaaaa agagaccggc gacgtcttcc atacttattc cgcttacgca
     3361 cgcggtcctg aagagttgct cggcttttac ttctacctcg atcgc
//
LOCUS       DATNOI010000218        10591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1414263,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000218 DATNOI010000000
VERSION     DATNOI010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414263"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VN968_08390"
     CDS             complement(<1..913)
                     /locus_tag="VN968_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HXL29292.1"
                     /translation="MSGPLPATLPHAIRTGSWLAREALTDARGLGRYADAFCAIAIAL
                     WLAPFAAHAARSHLPNPPARPHLPYPRLAFPLEISGGQYAPVGWVDIPGWSEDDHLLA
                     YKAFRASCRPISAQHALPADPKALGISLRDPCRAAKTLDLSDGVKARAFFEEHFLPLR
                     ISRLGEDSGFVTGYYEPVIDGSRTQTDVYTVPIYRRPSNLFVRGFKQSNIGLPNKGQV
                     FRKIGRRKLVPYYDRAEIEDGAIAGRGLEICWLKNQTDLLFTQIQGSARVRIEDGSTI
                     RINYDAHNGYPYTAVGRILIDRGIVPKD"
     gene            complement(837..1544)
                     /locus_tag="VN968_08395"
     CDS             complement(837..1544)
                     /locus_tag="VN968_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HXL29293.1"
                     /translation="MDIYTIIFLALAVFIFLRLRNVLGQRTGSERPPYDRTARNVVQG
                     AQDNNNVVPIPGAVIDQTPLPPTADVVPPSDRWKGLAEPGTPIAQGLDAIAAQDSSFD
                     PRHFLSGARSAYEMIVLAFANGDRRALKDLLSAEVYESFETAIKDREKLEQKTETRFV
                     SIDKAELVGAEARDRAAQLTVRFVSQMVSVTRDKAGTIVDGNPDKVTDITDVWTFARD
                     VTSRDPNWKLVGTGSAH"
     gene            2039..2518
                     /gene="secB"
                     /locus_tag="VN968_08400"
     CDS             2039..2518
                     /gene="secB"
                     /locus_tag="VN968_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HXL29294.1"
                     /translation="MTNGNGAPAEAAPPPQLNVLAQYTKDLSFENPNAPASLAPQAQQ
                     PAINIQINVSANNIAENEFEVTLSVEGKAENGGKVMFSFDLAYAGVFRLLNIPKQDLH
                     PMVMIECPRLLFPFAREIIATSVRDGGFPPLMLDPVDFVGLYRQNMERQAAQQARPS"
     gene            complement(2560..3255)
                     /gene="dnaQ"
                     /locus_tag="VN968_08405"
     CDS             complement(2560..3255)
                     /gene="dnaQ"
                     /locus_tag="VN968_08405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HXL29295.1"
                     /translation="MREIVLDTETTGLDPLRGDRLVEIGCVEIFNRMPTGQTFHRHLN
                     PERDMPAEAFAVHGLSSEFLADKPLFAHVVDDFLEFIADAPLVIHNASFDIGFINAEL
                     DRIKLPTIARDRLVDTLLLARRKHPGVSNRLDDLCSRYAIDNSRRTKHGALLDAELLA
                     EVYIDLIGARQSQLVLAAETRETRVGGDGEMPRRQRLAPLAPRVTEDDRAAHRAFVAT
                     LGDKPIWSDFLTA"
     gene            complement(3287..3886)
                     /gene="coaE"
                     /locus_tag="VN968_08410"
     CDS             complement(3287..3886)
                     /gene="coaE"
                     /locus_tag="VN968_08410"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133574.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HXL29296.1"
                     /translation="MLLLGLTGSIGMGKSTTAKLFAEAGVPVYDADATVHMLYEGEAA
                     PAIEAAFPGTTADGKVDRNKLSARVVHDPAAMKQLEQIVHPMLGASRQKFLDDAERSG
                     APVAVVDVPLLFETGGEKRVDAVVVVTTTPEIQHQRILARDNMTGEKLDAILARQLPD
                     AEKRRRADFVVDTSHGLDPVRARIQDILTEAVKMPQRRS"
     gene            complement(3922..4530)
                     /locus_tag="VN968_08415"
     CDS             complement(3922..4530)
                     /locus_tag="VN968_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HXL29297.1"
                     /translation="MTLWRGKHPLVLASQSLTRQALLANAGLDFEAVAAELDERAVQQ
                     ASGLLVPGDIAALLAREKALSVSSRQPGKYVIGADQTLSQGARLFSKPAGRPQAAEQL
                     RALAGRSHELHSAVAVARDGKILFEAVAIAGMTMRRLGEAEIDVYLDQAGEAVTSSVG
                     AYQLEGLGVHLFERIEGDHFTILGLPLLPLLAFLRSERLLGV"
     gene            complement(4527..5363)
                     /locus_tag="VN968_08420"
     CDS             complement(4527..5363)
                     /locus_tag="VN968_08420"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HXL29298.1"
                     /translation="MPTTGNYFHLHLVSDSTGETLITVARAVAAQYANVTPVEHVYPL
                     VRSQKQLDRVLSEIEEAPGIVLFTLLEKDLVGRLEAKCQQINIPSLSIIGPVMQLFQA
                     YLGSATTGRVGAQHTLNTEYFKRIDALNYTMMHDDGQHVEGLEEADVVLVGVSRTSKT
                     PTSIYLANRGIRAANVPLVPGIPLPHQLETLTKPLVVSLHATPERLIQVRQNRLLSMG
                     DRDNDTYIDRQAVADEVAFARRLSAKFNWALLDVTRRSIEETAAAIMKLFTDRQRQRL
                     AE"
     gene            5838..6263
                     /gene="hemJ"
                     /locus_tag="VN968_08425"
     CDS             5838..6263
                     /gene="hemJ"
                     /locus_tag="VN968_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HXL29299.1"
                     /translation="MPYEWIKALHVIAVISWMAGMLYLPRLFVYHCEAEAGSKQSETF
                     KVMERRLLKAIINPAMIVTWLAGLYLAWAGHWFSSGWLHGKLLLVVVLSGVHGFFSRC
                     VKDFAADRNLRSQKFYRIINEVPTVLMIGAVILVVVKPL"
     gene            6534..7799
                     /gene="rho"
                     /locus_tag="VN968_08430"
     CDS             6534..7799
                     /gene="rho"
                     /locus_tag="VN968_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXL29300.1"
                     /translation="MREMKLQDLKAQTPAELVSFAEEKGVENASTMRKQELMFAILKQ
                     LAIQETDIIGEGVVEVLSDGFGFLRSPDANYLPGPDDIYVSPSQIRRFGLRTGDTIEG
                     HIRSPKEGERYFALLKVNTLNFEDPEKSKHKVNFDNLTPLFPDQRFRLELEDPTRKDL
                     SARVIDIVAPIGKGQRALIVAPPRTGKTVLMQNIAHSITANHPECYLIVLLIDERPEE
                     VTDMQRSVKGEVVSSTFDEPAVRHVQVAEMVIEKAKRLVEHGRDVVILLDSITRLGRA
                     YNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALVDTGSRMDEVI
                     FEEFKGTGNSELILDRKVSDKRTFPAIDISRSGTRKEELITDPQLLKKMYVLRRILNP
                     MGTMDAIDFLLDKLRNTKNNSEFFESMNT"
     gene            8044..9438
                     /gene="mnmE"
                     /locus_tag="VN968_08435"
     CDS             8044..9438
                     /gene="mnmE"
                     /locus_tag="VN968_08435"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HXL29301.1"
                     /translation="MHPRDQTIFAVSSGRPPSAISIVRVSGPQAGVALTLIAGKMPVP
                     RMATRALLRDRDRQPIDDAMALWFPGPASATGEDVAEFHVHGGRAVLAALFAALSDFD
                     HVRAAEPGEFTRRAFENGKLDLTEAEGLDDLIHADTDRQRRQALRQLKGLLGDQARDW
                     RAQVIEASALIEAGIDFSDEADVPAELIAPALKKIKALLGEVQHVLAAQGHGERLREG
                     LVVAIAGPPNVGKSTLMNQLARREVAIVSPHAGTTRDVIEVQLDLDGYPVTVIDTAGI
                     RETADPVEQEGVRRAQARAAEADLVLWLVDAHDGKNLEGNLQQGAAPAWVVRNKIDLD
                     AVESDAAVLGIDAPNPRQPAAVLGQTRRHVDFKISASTGDGIQELIAALIAFAQNYFG
                     AGEGGLISRERQRKLLQETAVSLQRSLSVIPEGEELAAEDLRMAARSLGRLLGRVDVE
                     DVLDVIFREFCIGK"
     gene            9911..>10591
                     /locus_tag="VN968_08440"
     CDS             9911..>10591
                     /locus_tag="VN968_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL29302.1"
                     /translation="MIARPESFDVIVIGGGHAGCEAASAAARIGAKTALVTHRFATIG
                     AMSCNPAIGGLGKGHLVREVDALDGLMGRVADAAGIQFRMLNRRKGPAVRGPRAQADR
                     KLYAAAMQAAIRETANLCVIEGEADELIVTNGRVTGIHLADRSEFSAGAVVITTGTFL
                     HGLIHLGERNWPAGRVDEAPAMGLSKSFERAGFTLGRLKTGTPPRLDGRTIDWSAVEM
                     QPGDDPPEP"
BASE COUNT         2069 a         3218 c         3270 g         2034 t
ORIGIN      
        1 ggtccttcgg gacgatgccg cgatcgatca ggatgcggcc gaccgccgta tagggataac
       61 cattatgcgc gtcgtaattg atgcggatgg tcgagccgtc ctcgatgcgg acccgcgccg
      121 agccctggat ttgcgtgaac agcagatcgg tctggttctt gagccagcag atttcgaggc
      181 cccggccggc gatcgcgccg tcttcgattt cggcccgatc gtaatagggc acgagcttgc
      241 gacggccgat cttgcgaaac acctgtccct tgttgggcaa gccgatattg ctttgtttga
      301 agccgcggac gaacaagttc gaggggcggc ggtaaatcgg gacggtatag acgtcggtct
      361 gggtcctgga cccgtcgatg acaggttcat agtagccggt gacaaaaccc gaatcttcgc
      421 ccagccgcga gattcgcagg ggcagaaaat gctcctcgaa gaatgcccgc gccttgacgc
      481 catcggatag gtcgagggtc ttggccgcgc ggcagggatc gcgcaacgag atgcccaacg
      541 ccttgggatc ggcgggaagc gcatgttgcg ccgagatcgg ccggcagctc gcgcgaaacg
      601 ccttgtaggc caggagatgg tcgtcttcgc tccagcccgg gatatcaacc cacccgaccg
      661 gggcatattg accgccgctg atctcaagcg gaaaggcgag gcgcggatac ggaagatgcg
      721 gtcgcgcggg aggattgggc agatgagaac gcgcggcatg tgcggcaaac ggagccagcc
      781 acaatgcgat ggcgattgca caaaacgcgt cggcgtatcg ccccaagcca cgcgcgtcag
      841 tgagcgcttc ccgtgccaac cagcttccag ttcggatcgc gtgaggtaac gtcgcgggca
      901 aaggtccaga catcggtaat gtcggtgacc ttgtccggat tgccgtcgac aatcgtgccg
      961 gccttgtcgc gggtgaccga aaccatctgc gacacaaaac gaaccgtcag ctgcgccgcc
     1021 cgatcgcgcg cctccgcacc cacaagctcg gccttgtcga tcgaaacgaa acgggtttcg
     1081 gtcttttgtt cgagcttctc gcgatccttg atcgcggtct cgaaactctc atagacctcg
     1141 gccgatagca ggtccttcag cgcgcgacga tcgccattgg cgaacgccag cacaatcatc
     1201 tcataggcgc tgcgcgcgcc gctgaggaaa tgtcgcggat caaacgagga atcctgcgcg
     1261 gcgatggcat cgagcccctg ggcgatcggg gtgccgggct cggccaggcc cttccagcga
     1321 tcgctcggcg gcaccacatc cgccgtcggc ggtagcggcg tctggtcgat caccgcgccc
     1381 ggaatcggaa ccacattatt attgtcctgc gcgccctgca ccacgttgcg cgcagtgcga
     1441 tcgtagggcg gccgctcgct tccggtgcgc tgtccgagga cattacgcag acgcaggaaa
     1501 atgaagaccg ccagcgccag gaagatgatg gtgtaaatat ccacgtcgca ttcgctttct
     1561 ggtctgcgcc ggcaacggag ccggcggtag agcgttccct gaaggagaac ggcagagact
     1621 acatcatgga tgggtctgaa gcatgtaggc acgaaacctg gcctggccaa tggcgcgttt
     1681 ttggcttgaa aagccatacc ggtggaattg gccaagccaa ctgctcgcag cccgtttact
     1741 gctaacgaat tgtagcgcct tttggttgcg aggggaaatc cgatactgcc cggaaatcgc
     1801 gcatattcaa caatttagag tgcagcctcc gcagcggacc ggcccgcttg gccgggttct
     1861 gccgtgccgg cctgttcgcc aagcatcgcc ttggccggac aggggcaatt gtcatccttg
     1921 tggaatgagg cgaggctatg atagccactc gccccgacga gggcattttc gcaccccacc
     1981 agcgaattca ggcgcaaacc agtcccgcgc gccttagaat gttccaggag agacttccat
     2041 gaccaacggt aacggtgcgc ccgccgaggc ggcccctcct ccccagttga acgtgctggc
     2101 gcagtatacc aaggacctgt cgttcgaaaa tccgaacgcg ccagcctcgc tggcaccgca
     2161 ggcgcagcag cccgcgatca atatccagat caacgtcagc gccaacaata tcgccgaaaa
     2221 cgaattcgag gtgacgcttt cggtcgaagg caaggcggaa aacggcggca aggtgatgtt
     2281 cagcttcgat ctggcctatg ccggcgtatt ccgtcttctc aacataccga agcaagacct
     2341 gcatccgatg gtcatgattg aatgtccgcg gctgctgttt ccgttcgcac gcgaaatcat
     2401 cgcgacatcg gtgcgcgacg gcggattccc cccgctgatg ctggatcccg tcgattttgt
     2461 cggtctctac cggcagaata tggaacggca ggcggcccag caggccagac cgagctgaac
     2521 cggctcgcgc ctgggcgtta ctcgctgggc gttaactggt tacgcggtca ggaaatcgct
     2581 ccagatcggc ttgtcgccca gcgtggcgac aaaggcccga tgcgccgcgc gatcatcctc
     2641 cgtgacccgc ggcgcgagcg gcgccagacg ttgccgccgc ggcatttcgc cgtccccacc
     2701 aacacgcgtc tcgcgggtct ccgccgccag gaccagttgc gactgccgcg ccccgatcag
     2761 gtcgatgtag acttcggcca gaagttcggc gtcgagcaac gctccgtgct tggtgcggcg
     2821 ggaattgtcg atggcatagc gcgagcagag atcatcgaga cggttcgaaa cgcccggatg
     2881 ctttcgccgc gccagcagaa gcgtgtcgac cagccggtcg cgcgcgatcg tgggcagctt
     2941 gatgcgatca agctcggcgt tgatgaagcc gatgtcgaat gaagcgttgt ggatcaccag
     3001 cggcgcgtcg gcgatgaatt ccaggaagtc atcgaccaca tgcgcgaaca gcggcttgtc
     3061 ggcgaggaac tcactggata gaccatgcac cgcgaaggcc tcggccggca tgtcccgttc
     3121 cggattgaga tgccggtgaa aggtctgccc cgtcggcatg cgattgaaaa tctcgacaca
     3181 gccgatttca accagccggt cgccccgcag cgggtcgagg ccggtggttt cggtatcgag
     3241 aacgatttcg cgcatgcatc aggccgcaaa gttaaggaat aacgaatcag gatcgtcgct
     3301 gcggcatctt aaccgcctca gttagaatgt cctggattcg tgcgcgcacg ggatcaagcc
     3361 cgtgcgatgt atccaccaca aaatccgctc gccggcgctt ttcggcatcg ggcagttgtc
     3421 gcgccaggat ggcgtcgagc ttttcaccgg tcatgttgtc gcgtgccaga attcgctggt
     3481 gctgaatttc cggcgtcgtc gtcaccacca ccaccgcgtc gacccgtttc tcgccaccgg
     3541 tctcgaacaa cagcggcaca tcgaccaccg cgaccggtgc gccggaccgc tcggcatcgt
     3601 caaggaattt ttgacgggag gcgccgagca tcggatgaac gatctgctca agctgcttca
     3661 tcgcggccgg atcatgcacc actcgcgccg aaagcttgtt gcgatcgacc ttgccgtcgg
     3721 cggtcgtgcc cggaaacgcc gcttcgatgg caggcgccgc ctcgccttcg tacaacatgt
     3781 gaacggtcgc atcggcgtcg taaaccggaa cgccggcttc cgcgaacagt tttgcggtgg
     3841 tcgattttcc catcccgatc gagccggtca gcccaaggag caacattcaa cactcgccat
     3901 tcgctgcatg gtaaggaatt ttcaaacacc gagcagccgc tcgcttcgca ggaacgcgag
     3961 caatggcagc agcggcaaac cgaggatggt gaaatggtcg ccttcaatcc gctcgaacag
     4021 atgcaccccg agcccctcaa gctgataggc gccgacgctt gatgtgaccg cctctcccgc
     4081 ctgatccagg tagacgtcaa tttcggcctc gccaagccgc cgcatggtca tccctgcgat
     4141 cgcaaccgct tcgaacaata tcttgccatc gcgcgccacg gcaacggcgg aatgcagctc
     4201 atggctgcga ccggccaatg cgcgtagttg ctccgcggcc tgcgggcgac cggccggctt
     4261 gctgaagaga cgcgcgccct gcgacagcgt ctgatccgcg ccgatgacat atttgccggg
     4321 ttgccgtgac gataccgaaa gcgctttctc gcgcgcgaga agggccgcga tgtcgccagg
     4381 caccaaaagg ccggaagctt gctgcacggc ccgctcatcg agctcggccg ccacggcttc
     4441 gaagtcgagg cccgcattgg ccagcagcgc ctgccgcgtc aggctttgcg aggcgagaac
     4501 caacggatgt tttccgcgcc agagggtcat tcggctagcc gttgccgctg ccggtcggtg
     4561 aacagtttca tgatggcggc ggcggtctcc tcgatcgatc gccgcgtgac gtcgagcagc
     4621 gcccagttga acttggcgct caagcggcgc gcgaacgcga cttcatccgc gacggcctga
     4681 cggtcgatat aggtgtcgtt gtcccgatcg cccatgctca atagccggtt ctgccggacc
     4741 tggatcaggc gctcgggcgt cgcatggagg ctcaccacca gcggcttggt cagggtctcc
     4801 agttgatgcg gaagcgggat gccgggcacc agcggaacgt tcgccgcgcg gatgccgcgg
     4861 ttggcaaggt agatcgaggt cggggttttc gaggtgcggg acacgccgac cagaaccacg
     4921 tcggcctctt ccagcccttc gacgtgctgg ccgtcatcgt gcatcatcgt gtagttcagc
     4981 gcgtcgatgc gcttgaagta ttccgtattc agcgtgtgct gtgcgccgac gcgccccgtc
     5041 gtggcagaac cgagataggc ctgaaacagc tgcatgaccg gcccgatgat cgaaaggctg
     5101 ggtatgttga tctgctggca cttggcttcc agccggccga ccagatcttt ttccagcaag
     5161 gtgaacagca cgatgccggg cgcttcctcg atttcactca gcacccgatc gagttgcttc
     5221 tggctccgca ccagcgggta gacatgctcg accggcgtca cattggcata ttgcgcggcc
     5281 accgcgcgcg ccaccgtgat cagcgtctcg ccggtcgaat cggaaaccaa gtgaagatga
     5341 aaatagttgc cggtcgtggg caagggaacc cctgtgtatc ctgtgaatcg ctgtggagct
     5401 tttccacgag aatcgtgcga cggccgatcc atcggggata acccggcttt ttcttcacag
     5461 ggctcgaata aggataagtc cagtccacgg cggcaatgat agtgaggttc tgtggacttg
     5521 tctcttgata atcggcttac agccatgcgc aagtgcttga gcgagcgtga cgttatcgac
     5581 atcaccgttc gagctgaaaa attgtggatg gatgtgaaca agcccggaga attgcgggac
     5641 agttttgcgt gggcccaatc caccgttact tagactcaaa cctaagattc taaaattatt
     5701 gttttagaaa agtgccgctt gcggaaaggc ttccattcgg gtctttaggc gacatgcttt
     5761 tccaacgcga tcatccgtcg aaacgtttca cgttgatcgg ttggaaattc cagaccatcg
     5821 cgggcgaata gaattccatg ccgtatgaat ggatcaaggc gctgcatgtg atcgccgtca
     5881 tctcctggat ggcgggcatg ctgtatctgc cgcggctgtt cgtttatcac tgcgaggcgg
     5941 aagccggatc gaagcagtcc gaaactttca aggtgatgga acggcggttg ctgaaggcga
     6001 tcatcaaccc tgcgatgatc gtgacctggc tggccgggct ttacctcgcc tgggccggcc
     6061 attggttttc atcgggctgg ctgcacggca agcttctgct ggtggtggtg ctgtcgggtg
     6121 tccacggttt tttttcccgc tgcgtcaagg attttgccgc cgaccgcaat ctgcgaagtc
     6181 agaaattcta tcgtattatc aatgaagtac ccacggtcct catgatcggg gcggttatcc
     6241 tggtagtggt taagccgctt taacaggcac ccatgccaat gcggtgcccg ccaagtctcc
     6301 ttcaagtctc cttgcagaaa gctgaccgat tttctatatt gtcaaaatcc caccccacgc
     6361 aggcggatgt ggttgcgttc cggtttcctc gttgaaccgg ccgccacatc aggtttgaag
     6421 cctctccggc cccttcctcg ctcagacctg cggaccttct ttcttttcgg gcgtccgtat
     6481 tttcttaaag ccacctcgcg atccctccct agttattcca caggaccatc ccaatgcggg
     6541 aaatgaaact tcaagacctc aaggctcaga caccggccga actcgtttcg ttcgccgaag
     6601 agaaaggggt cgaaaacgcc agcacgatgc gcaagcagga gctgatgttc gccatcctca
     6661 aacaactcgc gatccaggaa accgacatta tcggtgaagg cgtcgtcgag gttctctcgg
     6721 acggatttgg attcttgcgt tcgccggacg ccaattatct gccggggccc gatgacatct
     6781 atgtctcgcc ctcccagatc cgccgcttcg ggcttcgcac cggcgatacc atcgaaggcc
     6841 acattcgcag cccgaaagaa ggcgaacgct attttgcgct gctgaaggtc aacacgctga
     6901 attttgaaga cccggagaaa tccaaacaca aggtcaactt cgacaatctg acgccgctgt
     6961 ttcccgacca gcggttccgg ctcgagctcg aagacccgac cagaaaggat ctttctgcaa
     7021 gggtgatcga catcgtcgcg ccgatcggca agggccagcg cgccctgatc gtggcgccgc
     7081 cacgcaccgg caagaccgtg ctgatgcaga acatcgctca ctcgatcacc gccaatcatc
     7141 cagagtgcta tctgatcgtg ctcctgatcg acgagcgccc ggaagaagtc acggacatgc
     7201 agcgctcggt gaagggcgaa gtcgtctcat cgacgttcga cgagccggcg gtgcgtcacg
     7261 tccaggtcgc cgagatggtg atcgagaaag ccaagcggct ggtcgagcat ggtcgcgacg
     7321 tcgtcatcct gctggactcg attacccgtt tgggccgggc ttacaacacc gtggtgccgt
     7381 catccggcaa ggtgctgacc ggcggcgttg acgccaacgc gctgcagcgg ccaaagcgtt
     7441 tcttcggcgc cgcgcgcaac atcgaagagg gcggttcgct caccatcatc gcgaccgcgc
     7501 tggtcgatac cggcagccgc atggacgaag tgatctttga agaattcaag ggcaccggca
     7561 attccgaatt gattctcgat cgcaaggttt ccgacaagcg gaccttcccc gcgatcgaca
     7621 tctcacgctc cggcacccgc aaggaagagc tgatcaccga tccgcaactc ctgaagaaga
     7681 tgtacgtgct gcgccggatc ctcaatccga tgggcacgat ggacgcgatc gactttctgc
     7741 tcgacaagct gcgcaacacc aagaacaact cggagttctt cgagtcgatg aatacctgaa
     7801 ctggctctcc ggctttgaag gggcgtcctc gcgcgagcgg aggcgccctt ttttcgtgcc
     7861 cggttcaagg cgcccgcgtg gccggtccga ctgttcgtgc tatgttctca aacgctgttc
     7921 gagcgcttta tcgaggcgcg caggagggcc actgttcgac aacgccgcat cccgcgtttt
     7981 gaatccgcga cccctgcgca aattttcgtt ggcttcgcaa gctggccctg gagcaaatag
     8041 gcgatgcatc cgcgcgacca aaccatcttc gctgtgtcat cgggccggcc gccaagtgcg
     8101 atttcgattg tgcgcgtatc cggcccgcag gccggggtgg cgctgaccct gatcgccggc
     8161 aaaatgccgg tgccgcggat ggccacgcgt gcgctgctgc gcgatcgcga tcgccaaccg
     8221 atcgacgatg cgatggcgct ctggtttccc ggaccggcga gcgcgaccgg agaagacgtc
     8281 gccgagtttc acgtccatgg cggtcgcgcc gttctcgcgg cgctgttcgc ggcgctctcg
     8341 gatttcgacc atgtgcgcgc ggcggagccg ggcgaattta cccgccgcgc attcgagaac
     8401 ggcaagctag atcttaccga ggccgagggt ctcgacgatc tgattcacgc cgacactgac
     8461 cggcagcgcc gccaggcgct gcgtcagcta aaaggcctgc tcggcgacca ggcgcgcgac
     8521 tggcgcgcgc aggtcatcga ggcctcggcg ctgatcgaag cggggatcga tttctccgac
     8581 gaagccgacg tgcctgcgga gctgatcgcg ccggcgctga aaaaaatcaa agcgctgctc
     8641 ggcgaagtcc agcacgtgct ggcggcgcaa gggcacggcg aacggctgcg cgagggactg
     8701 gtggtcgcga tcgcagggcc gccgaacgtc ggcaagtcga cgctgatgaa tcagctggcg
     8761 cggcgcgagg tggcgatcgt ctcgccgcac gccggcacca cgcgtgacgt catcgaagtg
     8821 cagctcgatc tcgacggcta tccggtgacg gtgatcgaca ccgccggcat ccgcgagacc
     8881 gccgatcccg tagagcagga aggcgtgcgc cgcgcccagg cgcgcgcggc cgaagcagac
     8941 ctcgtgctgt ggctggtcga tgcgcatgac ggaaaaaatt tggaaggaaa tttgcagcag
     9001 ggtgccgcgc cggcgtgggt ggtgcgcaac aagatcgatc tcgacgcggt cgaatccgat
     9061 gccgcggtgc tgggaatcga cgcgccgaat cccagacaac ccgcggctgt cctggggcag
     9121 acccggcgcc atgttgactt caaaatctcg gccagcacgg gtgacggcat tcaggaattg
     9181 atcgccgctc tgatcgcgtt cgcgcaaaat tattttggtg cgggggaggg cggcctgatc
     9241 agtcgggaac ggcagcgcaa acttctgcag gagacggccg tgtcgctaca gcgcagcctg
     9301 tcggtgattc cggaaggtga ggaactggcg gcggaggatt tgcggatggc ggctcggtcg
     9361 ctcggcaggc tgctcggccg ggtcgacgtc gaggatgtcc tggacgtgat cttccgcgaa
     9421 ttttgtatag gtaaatgaga ttgtctcatt aacgttaacg aaacgtttcg tgtgaaaccg
     9481 tttcacgtga aacacaaaac taaagataaa aaattgtgtc gcgcctctcg agagcgagat
     9541 gggttttgag cgacagcaac agaaatgcgc tccctcccgc ttgctgggtc gaggcgagcg
     9601 aagcttgctc tgtaaggctg gggtgggggt gcagtgcaat tgcttggcaa gcagttccga
     9661 ccgacccccg cgctcgatca agcgcaatag ccgaagcctc gcttcgtcgt tcttatctaa
     9721 ggacggccgc taaaggcggt ccacgcttct ccgcaaagcg ggagaggtga actgaatccg
     9781 cggactgacg gattccatca aaaacatccc tctctcgcga gctgatgttt ggcggggatg
     9841 tttcacgtga aacaccccgc gataaactta cggcttccga gattcgtcgc cccgcgataa
     9901 aagctccggc atgatcgccc gaccggaatc gtttgacgtt attgtcattg gaggaggcca
     9961 cgccggctgc gaagccgcca gcgccgccgc ccgcatcggt gcgaagaccg cgctggtgac
    10021 ccaccgcttt gcgaccatcg gcgcgatgtc ctgcaatccc gcgatcggtg gtctcggcaa
    10081 gggccatctg gtccgcgagg tcgatgcgct ggacgggctg atgggccgcg tggccgatgc
    10141 cgcggggatt cagtttcgca tgctcaatcg ccgcaagggt ccggcggtgc gcggtccgcg
    10201 cgcccaggcc gaccgcaagc tctacgcggc ggcgatgcag gccgccatcc gtgagactgc
    10261 gaacctctgc gtgatcgaag gcgaggcgga cgaactgatc gtgacgaacg gtcgcgtcac
    10321 gggcattcat ctcgctgata gaagcgagtt ttcggccggt gcggttgtca tcaccaccgg
    10381 gacctttctg catggactga tccatctcgg cgagaggaat tggccggccg gccgcgtcga
    10441 tgaggctcca gccatgggac tctcgaagtc gttcgagcgt gccggcttca cgctcggacg
    10501 gttgaagacc ggcaccccgc cgcggctcga tggccgcacc atcgactggt ctgcagtcga
    10561 gatgcagccg ggcgacgatc caccggagcc g
//
LOCUS       DATNOI010000219         2290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_936974,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000219 DATNOI010000000
VERSION     DATNOI010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936974"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /gene="glnT"
                     /locus_tag="VN968_08445"
     CDS             complement(<1..1076)
                     /gene="glnT"
                     /locus_tag="VN968_08445"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HXL29303.1"
                     /translation="MTIDLVQAAKEKGIRYFLISFTDLMGTQRAKLVPAEAIGQMVKS
                     GAGFAGFATWLDMTPADPDMFAIPDPDSLVQLPWKPEVGWLAADPWMNGKPVEQAPRN
                     LLKRLKAKAAESGMQLKSGVECEFFLISPDGAGIRDSADRQAKPCYDQSALMRRYDVI
                     SEICDGMLKLGWKPYQNDHEDANGQFEMNWEYDDALVTADRHAFFKYMVKSIAEKHEM
                     RATFMPKPFSHLTGNGCHMHVSLWKGAQNLFEDEQGELGVSALGYHFIGGIIHSADAL
                     AAILNPAVNSYKRINAPRTLSGATWSPNSVTYSGNNRTHMIRIPEAGRFELRLADGAA
                     NPYAMPAAILAAGLDGINAKRDPG"
     gene            complement(1109..>2290)
                     /locus_tag="VN968_08450"
     CDS             complement(1109..>2290)
                     /locus_tag="VN968_08450"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HXL29304.1"
                     /translation="LLFLGASISRYPLEGYREKCGTDVVLGSRFAKKPIHLRTPVTIA
                     GMSFGALSGAAKEALGRGASAVGTSTTTGDGGMTAEERGHSSLLVYQYLPSRYGMNPD
                     DLRRADAIEIVIGQGAKPGGGGMLLGQKISERVAKMRTLPEGIDQRSASRHPDWTGPD
                     DLEIKIEELREITDWEKPIYVKVGASRPYYDTALAVKSGADVVVIDGMQGGTAATQEV
                     FIENVGIPTLAAIRPAVQALEELGMHRKVQLIVSGGIRNGADVAKALALGADAVAIGT
                     AALIALGDNSSQLEAEYQALGTTAGAYDDWHEGRDPAGITTQNPELAKRLDPVLGGRR
                     LANFLSVLTLETQTIARACGKSHVHNLEPEDLVALTVEAAAIARVPLVGTDWIPGQVR
                     Y"
BASE COUNT          366 a          726 c          750 g          448 t
ORIGIN      
        1 ttgcccgggt cgcgcttggc gttgatgccg tcgaggcccg ccgccaggat cgccgccggc
       61 atggcgtagg gattggcggc gccgtcggcg aggcgaagtt caaatcgtcc ggcttccgga
      121 atgcgaatca tgtgggtgcg gttgttgccc gaataggtca ccgaattcgg cgaccaggtt
      181 gcgcccgaca gcgtccgcgg cgcgttgatc cgtttgtagg aattgaccgc cggattgagg
      241 atcgcggcca gcgcgtcggc ggaatgaatg atgccgccga tgaagtggta gccgagcgcg
      301 gagacgccga gttcgccttg ctcatcttcg aacaggttct gggcgccctt ccacagcgac
      361 acatgcatgt ggcacccatt gccggtcaga tgcgagaacg gctttggcat gaaggtcgcg
      421 cgcatctcgt gcttttccgc gatcgacttc accatgtatt tgaagaacgc gtgccgatcc
      481 gccgtgacca gggcgtcatc atattcccag ttcatctcga actgcccgtt ggcgtcttcg
      541 tggtcgttct ggtacggctt ccagccgagc ttgagcatgc cgtcgcagat ctcgctgatg
      601 acgtcatagc gccgcatcag cgcggactgg tcataacaag gtttggcctg ccggtcggcg
      661 ctatccctga tgccggcgcc atcaggcgag atcaggaaga attcgcattc cacgccgctc
      721 ttgagctgca taccgctttc ggcggccttg gccttcagtc tcttgagcag gtttcgcggc
      781 gcctgttcga ccggcttgcc gttcatccag gggtcggcgg cgagccagcc gacttccggc
      841 ttccagggca gctgaacgag gctgtccgga tcgggaatgg cgaacatgtc gggatccgcc
      901 ggcgtcatgt cgagccaggt cgcaaagccg gcgaagccgg cgccgctttt caccatctgg
      961 ccgatcgcct cggctggcac cagcttggcc cgctgggtcc ccatcaggtc ggtaaacgaa
     1021 atcaggaagt atctgatgcc cttttctttg gccgcctgca cgagatcgat tgtcatgggg
     1081 tcctcttgat gaccatggga agggggcgtc agtatctgac ttgacccggg atccaatcgg
     1141 tgccgaccag cgggacgcgg gcgatcgcgg cggcttccac cgtgagcgcg acgagatctt
     1201 ccggttcaag attgtggacg tggctcttgc cgcaggcgcg ggcgatagtc tgggtttcca
     1261 gcgtcagcac cgagagaaaa ttggccagtc gccggccgcc cagcacggga tcgagccgct
     1321 tggccagttc gggattctgc gtcgtgatcc cggcgggatc gcggccttcg tgccagtcgt
     1381 cataggcgcc ggcggtggtg ccgagcgcct ggtattccgc ctccagctgc gaactgttgt
     1441 cgccgagcgc gatcagcgcc gccgtgccga tggcgacggc gtcggcgcca agcgccagcg
     1501 ccttggccac gtcggcgcca ttgcggatgc cgccggagac gatcagttgc accttgcggt
     1561 gcatgccgag ttcctccagc gcctgcaccg cgggccggat cgccgccagc gtcgggatgc
     1621 cgacattctc gataaacact tcctgcgtcg ccgccgtgcc gccctgcatg ccgtcgatca
     1681 cgaccacgtc cgcgccggac ttcacggcaa gcgcggtgtc gtaatagggg cggctggcgc
     1741 cgaccttgac gtagatcggc ttttcccaat cggtgatctc gcgaagttcc tcgatcttga
     1801 tttcgagatc gtcgggaccg gtccagtccg gatgccgcga cgccgagcgc tggtcgatgc
     1861 cttccggaag ggtccgcatt ttcgccaccc gctcggatat tttctggccg agcagcatgc
     1921 cgccgccgcc gggcttggca ccttgcccga tcacgatctc gatggcgtca gcgcgccgca
     1981 aatcgtcggg attcatgccg tagcgcgacg gcagatattg atagaccagc aacgaggaat
     2041 ggccgcgctc ttccgccgtc atgccgccgt cgccggtggt ggtgctggtg ccgaccgcgg
     2101 aggcgccgcg tccaagcgct tccttggcgg cacccgagag ggcgccaaaa ctcatgccgg
     2161 cgatcgtcac cggcgtcctg agatgaatag gcttcttggc gaagcgcgac cccagcacga
     2221 cgtcggtgcc gcacttctcg cggtagcctt cgagcggata gcgcgagatc gatgcgccga
     2281 gaaacagcag
//
LOCUS       DATNOI010000220         3178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1818762,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000220 DATNOI010000000
VERSION     DATNOI010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818762"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VN968_08455"
     CDS             <1..871
                     /locus_tag="VN968_08455"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXL29305.1"
                     /translation="GAKGFLGKLAALAKARPAYFKPIIGLERGLTDPITELNMGQTAE
                     VVGHLFAITRAQSDAYAVESHKRLANAQAQGWLKGEVETAFARDGKFYEHDDGVRPDS
                     SPESLAKLKPVFERPWGQVTAGNSSQITDGASWTILASEEAVAKHGLAPKAVIVDSQW
                     SALDPSIMGLGPVLSATELLKRNKLSLGDIETWELNEAFATQVLGCLAAWNDDKFCRE
                     ILGLEGAAGQIDQAKLNVDGGAISLGHPVGCSGNRIVLHLVNAMKRLGTKRGIATECI
                     GGGQGGAMLIETV"
     gene            880..2976
                     /locus_tag="VN968_08460"
     CDS             880..2976
                     /locus_tag="VN968_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXL29306.1"
                     /translation="MDSRIMDVLGDRVLELGPKPDASALYRNFKLTRDDDGIAWLLFD
                     RAGASANTLSADVIEELDSVLAALESQRPSGLVIRSAKPSGFIAGADVNEFRGASDPR
                     PVETAIGRAHAVIDRLEALKVPTVAVIHGFCLGGGLEVALACQSRIAIDGARFGFPEV
                     MLGLHPGLGGPARFTRLVNPMQAMTLMLTGKTIDARKAKSLGLVDAVAQERHVRNAVR
                     DAVFGRLKRAHPGVLNTVLNSGPVRGFLGSRMRAEAEKAAPHESYPAPYALIDLWEKH
                     GGDKTAMLAAEKTSFANLMVTPTAQNLIRVFFLREQMKKLAGSGNQISHVHVIGAGAM
                     GGDIAAWCANQGMRVTLSDMKAEPIAGAMKRASDLFGKIMRKHTDIRDALDRLVPDLD
                     GEGIRNADLIIEAVPEKLELKQKIYAGLEPKMKPGAILATNTSSIPLQDLRTTLKSPE
                     RLVGLHFFNPVSRLQLVEVVSHDGTDPQLLKQALAFVGAIDRLPLPVKSSPGFLVNRA
                     LTPYMLEAMVMLDEQIDKRTIDAAAEKFGMPMGPIELADQVGLDICLAVGDMLRSKFG
                     DSLPPTPAWLREKVAAGDLGRKTGRGFYVWKDGKPDKMSRSPATVQPTAEMIDRLMLP
                     MSNVCVACLREGIVDNADVVDGAMIFGTGYAPFRGGPLNYARTRGPENVASALRALAQ
                     KFGGRFTPDAGWDSFK"
BASE COUNT          606 a         1004 c         1025 g          543 t
ORIGIN      
        1 cggcgccaag ggatttctcg gcaagctggc ggcgctcgcg aaagcacggc cggcttattt
       61 caagccgatc atcggccttg agcgcgggct gaccgatccg atcaccgagc tcaacatggg
      121 ccagacggcg gaagtggtcg gtcatctgtt cgccatcacg cgcgcgcaat cggatgccta
      181 tgcggtcgaa agccacaagc ggctggccaa cgcgcaagcg caaggctggc tgaagggcga
      241 ggttgaaacc gcgttcgcac gcgacggcaa gttctacgaa catgacgacg gcgtgcggcc
      301 ggattcctca cccgagagcc tcgccaagct gaagccggtg ttcgagcggc cctggggtca
      361 ggtcaccgcc ggcaactcct cgcagatcac cgacggcgca tcgtggacga ttctcgcatc
      421 cgaagaggcc gtcgccaagc atggtctcgc gccaaaggcc gtgatcgtcg acagccaatg
      481 gtcggcgctc gatccctcta tcatgggcct cgggccggtg ctgtcggcga ccgaactgtt
      541 gaagcgcaac aaattgtcgc tcggggatat cgagacctgg gaactgaacg aggcgttcgc
      601 gacgcaagtg ctgggctgtc tcgccgcctg gaacgacgac aaattctgcc gcgagatttt
      661 gggtctcgag ggggccgccg ggcaaatcga ccaggcaaaa ctgaatgtcg atggcggcgc
      721 gatcagcctt ggtcatcccg tgggctgcag cggcaatcgc atcgtgctgc atctcgtcaa
      781 cgcgatgaag cggctgggca ccaagcgcgg catcgccacc gaatgtatcg gcggcgggca
      841 gggcggcgcc atgttgatcg agacggtata gggaacgtca tggatagcag gatcatggac
      901 gttctcggcg accgcgtgct cgaactcggg ccaaaacccg acgccagcgc gctttatcgc
      961 aatttcaagc tgacccgcga tgacgacggc atcgcctggc tgctgttcga ccgcgccggt
     1021 gccagcgcca acacgctgtc ggccgacgtc atcgaagaac tcgattccgt gctggcggcg
     1081 ctggaaagcc agcggccgtc cgggctggtc attcgctccg cgaagccgtc cggcttcatc
     1141 gccggcgccg acgtcaacga atttcgcggc gccagcgatc cgcggccggt cgagaccgcg
     1201 atcggccgtg cccatgcggt gatcgaccgc ctcgaagcct tgaaggtccc gaccgtcgcc
     1261 gtgatccatg gcttctgcct cggcggcggc ctcgaagtcg cgctcgcctg tcagtcgcgc
     1321 attgcgatcg acggcgcccg ctttggtttt cccgaagtga tgctcggcct gcatcccggc
     1381 ctcggcgggc ccgcgcgttt cacgcgtctg gtcaatccga tgcaggcgat gaccctgatg
     1441 ctgaccggca agaccatcga tgcgcgcaag gcaaagtcgt tgggcctcgt cgatgccgtg
     1501 gcgcaggagc ggcatgttcg caatgctgtc agggacgcgg tattcggccg cctgaaacgc
     1561 gctcaccccg gcgttctgaa taccgtcctg aattccggtc ccgtccgcgg ctttctcggt
     1621 tcgcggatgc gcgcggaggc tgaaaaggcc gcgccgcatg agagctatcc tgcgccttac
     1681 gcgctgatcg atctctggga aaagcacggc ggcgacaaga ccgcgatgct ggcggccgaa
     1741 aagacctcgt tcgccaatct gatggtaacg ccgaccgcgc agaacctgat ccgggttttc
     1801 ttcctgcgcg agcagatgaa gaagcttgcc ggcagcggca accagatcag ccacgttcac
     1861 gtcattggcg cgggcgcgat gggcggcgac atcgcggcgt ggtgcgccaa tcagggcatg
     1921 cgggtcacgc tatcggacat gaaggcggag ccgattgccg gcgcgatgaa acgcgcatcg
     1981 gatctgttcg gcaagatcat gcgcaaacac accgatatcc gcgacgcgct ggatcggctc
     2041 gttcccgatc tcgacggcga gggcatccgc aacgccgacc tcatcatcga agcggtgccg
     2101 gaaaagctgg agctgaagca gaagatctat gccgggctcg agccgaaaat gaaacccggc
     2161 gcgatcctcg ccaccaacac ctcgagcatt ccgctgcagg atctgcgcac gacgctgaaa
     2221 tcgccggagc ggctggtcgg attgcacttc ttcaatccgg tgtcacgcct gcaactggtc
     2281 gaggtcgtca gtcatgacgg caccgacccg caactgctga aacaggcgct tgccttcgtc
     2341 ggcgccatcg accggctgcc actgccggtg aagagttcgc cgggttttct cgtcaaccgc
     2401 gcgctgacgc cttacatgct cgaggcgatg gtgatgctgg acgagcagat cgacaagcgc
     2461 accatcgacg ccgcagcgga aaaattcggc atgccgatgg ggccgatcga gctcgccgat
     2521 caggtcggcc tcgacatctg cctcgcggtc ggcgacatgc tgcgctcgaa attcggcgat
     2581 tccctgccgc cgacgccggc gtggctgcgc gagaaagtgg cggccggcga tctcggccgc
     2641 aagaccggca ggggtttcta tgtctggaaa gacggcaagc ccgacaagat gtcccgttcg
     2701 cccgcgacgg tacagccgac ggcggaaatg atcgaccggc tgatgctgcc gatgtcgaac
     2761 gtctgtgtcg cctgcctgcg cgagggcatc gtcgacaatg ccgacgtggt cgatggcgcc
     2821 atgatcttcg gcaccgggta tgcgccgttt cgcggcggtc cgctgaacta tgcgcgaacc
     2881 cgcgggcctg aaaacgtggc gtcggcgttg cgcgcgctgg cgcaaaagtt cggcggccgg
     2941 ttcacgcccg acgccggctg ggatagtttc aaataataga gcatgatccg gaaaagtggg
     3001 taccggtttt ccgaaaagat catgctcaaa caaaaaaata gagcgggatg acgattcgaa
     3061 gaagagtcat cccgctctag gatttccgat ccgaccgacg gggcgctcct catgaccgac
     3121 acccaagccg ttcacgcccc gccggattcg gaaaccgagc ccctcggcga tctgtcga
//
LOCUS       DATNOI010000221        11837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2191001,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000221 DATNOI010000000
VERSION     DATNOI010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2191001"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VN968_08465"
     CDS             <1..370
                     /locus_tag="VN968_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXL29307.1"
                     /translation="PALNDAEIERILDAAAHAGVKEASYVLLRLPLEVRDLFREWLMA
                     NYPDRYRHVFTMIRDMRGGRDYDSQWGTRMKGTGPMAWMIGRRFEIACEKLGLNKRRS
                     KLTTDHFARPKRSGQQLSLF"
     gene            391..846
                     /locus_tag="VN968_08470"
     CDS             391..846
                     /locus_tag="VN968_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL29308.1"
                     /translation="MNQKPPAPVARLTMITLGVGNMRASIAFYEALGFARKFRATGEA
                     VAFFETGGTAIGLFPWHQLAQDVTLPEDPRPQAFRGVTLAWNCRSTEEVDAVLNFAIS
                     KGASLLKPAHKTDYGGYSGYFVDPDGHPWEVVVAPSIEVGEDRRIHLPD"
     gene            complement(890..1072)
                     /locus_tag="VN968_08475"
     CDS             complement(890..1072)
                     /locus_tag="VN968_08475"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HXL29309.1"
                     /translation="MSNLEPLPVLIKLYPNRRLHDGEIGRYRSLDELRVWRERQVPFL
                     IVDSKTGDDVTEKILS"
     gene            1210..1947
                     /locus_tag="VN968_08480"
     CDS             1210..1947
                     /locus_tag="VN968_08480"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HXL29310.1"
                     /translation="MIREKSGNDQTRLPNGVIAIAPPSFRRERALIKRGVWPVAGCDE
                     AGRGPLAGPVVAAAVVLDPKRIPKGIDDSKRLTPERREELFEEICLTASFAVAFASPA
                     RIDRDNILRASLWALARAVHALPEMPRHVFVDGRDRLDTACDCEAVIGGDGLVLSIAA
                     ASIIAKVARDRLMCKLAQDCPGYGFESHKGYAVPEHLEALTRLGPTAHHRRLFAPVVA
                     AREKHEAVAGRPVRTQTEAGIEVSAEA"
     gene            complement(1980..4205)
                     /locus_tag="VN968_08485"
     CDS             complement(1980..4205)
                     /locus_tag="VN968_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL29311.1"
                     /translation="MHQRTIRFWLTCLVIGCVLPATLGSVFLFTISYQQQRAILERNT
                     ITTARALMQAIDAELFGAQSALQVLAASDRLASGDLAGFYLQASEALPNIGGNYIAIT
                     DSTGQQRLNTLKPFGDPLPQVLPSAKMRRVFENGKPAISDFLVVPDVGQSAITLEVPV
                     FSNGKVIYSLAMGIFGDRLGDILRRQNLPLDWITAILDGRGTIAARTHLPERFVGGKG
                     AAAFLVRSANRAGGITEADSLEGTPVLSAYNRSPRSGWAVAIGIPRTSLTGNPWRSLG
                     FNAAVTLALLALSIWMAKVISVRINRSIRSLHVPALALGTPELLSIPDNEIVEVNDVG
                     QALMKASRMIRERVVEREQGELATREMILAKQIADRTSRAKSEFLASMNHELRTPLNA
                     ISGFAQLLGRSGDTLEREKRIRYAQSIMEASTQLEELIDDVLDMARFETSEVDVNCEV
                     LDCLEVMTEVSRSLEVSARKKGVLFTVDVSGNLPAIVADRGRLIQVLLNLGSNAIKYN
                     VEGGWVLLTAFPCDDVVRFIVRDTGKGIPAERHGEIFEPFNRPGAELTQKEGTGIGLT
                     ISRRLVQAMDGKIGFESSVGRGSKFWVELPVAGAIAAKMARAPSLFATAADTRCKILY
                     IEDKIPNVEMMRAIIEDLSNTRFVDAQTVEEGLRIARSLRPDLVITDIHLPDGKGFDV
                     LRGLRKDDRTSQIPVVALTADTMPSNLHNMELAGFDHILTKPLKMPDLMKILRATLKA
                     A"
     gene            4949..6457
                     /locus_tag="VN968_08490"
     CDS             4949..6457
                     /locus_tag="VN968_08490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXL29312.1"
                     /translation="MRFTSLVIELIRARPRLVVWLVVLLQAALWLMLPLLLYGSPPGD
                     LATVLAYGREYQVGTDLGPPLAFWLGDIAFRAAGNHMFGVYLLAQLCAVATFWTLFLL
                     ARAVVGGQQAVLAVLLTMTVTAFGSPGVEFGPLVLARPLWALLLLHSWQLIGQGRHNA
                     WFAWSIEAGLLLLTTSAALGLLILVAGFAVATLRGRRTLTSFDPLYALLVIVVLALPY
                     LIWLIRADTLALPPWPAIADLSDRALHWGDLLGGLLLAISGIVLLVVINSAWFGRNPE
                     EAPIIYRPPVEPLARDFVYFFAVAPALAGSLISGFFNFDHVAGGAGIALLMSGLAVIV
                     ATGDLVHLRRQRVLRLVWAAAVVAPALAVILTALLLPWTGSAEEATSLPATAIAHFFG
                     DSFERRTNRPLRAVTGDPQLASLIALDTARPHLLLDTAPERTPWLTLAKFSETGGVVV
                     WRASDTVGTPPADIAQRFPGLVPEVPRVFEWPVTGRQPLLRIGWAIVRPKTP"
     gene            complement(6625..7443)
                     /locus_tag="VN968_08495"
     CDS             complement(6625..7443)
                     /locus_tag="VN968_08495"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXL29313.1"
                     /translation="MTPDNAPTVQQLLAFYLEAGVDCALTEAPVNRLSDPDIIPLSSE
                     AAPPKPVRTVPAAIPAARGEAAPAPEAAILSAREAARTAPSLEVLRALLENFDGCALK
                     STATRLVFADGNPQARIMFVGEAPGREEDIEGLPFVGRSGKLLDRMIAAIGLDRSSAY
                     IANVIPWRPPGNRTPTPQETQICLPFIQRQIELVNPDVLVTLGNPSTQTLLSTREGIM
                     KTRGRWFDYDTGTRMIRALATFHPAYLLRSPSYKRMAWQDLRSIAKALEHSSTG"
     gene            7589..9247
                     /locus_tag="VN968_08500"
     CDS             7589..9247
                     /locus_tag="VN968_08500"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HXL29314.1"
                     /translation="MSTEELPPRESMEFDVVIVGAGPSGLAAAIRLKELNADLTVVVV
                     EKGSEVGAHILSGAVIDPVSLDKLIPDWREDADCPLKTQVKADKFYWMTTGMAIPLPA
                     IAMPPLMNNHHCYIGSLGNVCRWLARKAEALGVEIYPGFAATEVLYDEKGAVRGIATG
                     DMGVAKDGSHKSSYTRGMELVGKYTLFAEGARGSLTKQLIARFSLDAGSEPPKFGIGL
                     KEVWQIDPARHQKGLIQHSFGWPLANSTGGGSFLYHYDDNLVAVGFVVHLNYDDPYLS
                     PFDEFQRFKTHPAIRTLFEGGKRLAYGARAITEGGYQSVPRLSFKGGALIGCAAGFVN
                     VPRIKGVHNAVGSGMLAAQHVNAALGSGRANDELVDYENAWRDSPVGKDLYPVRNVKP
                     LWSKFGTVVGVALGGIDMWCNTLGFSLFGTQSHAKPDRKTLDPAKAHAPIAPPKPDGK
                     LTFDKLSSVFLSNTNHEEDQPVHLKVADMNLQKTSEHDVYAGPSNRYCPASVYEWVEE
                     SAGPRFQINAQNCVHCKTCDVKDPNGNITWVPPEGGGGPNYEAM"
     gene            9490..11274
                     /locus_tag="VN968_08505"
     CDS             9490..11274
                     /locus_tag="VN968_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL29315.1"
                     /translation="MFSIRFNRWTIAAVAFTVLAVPASVSAQTPDHPADNSAQFPSRG
                     DLKALTTSGSYLAARHASVERDASSAAAFYRSALRTDPKNNELMDRAFISSLADGDID
                     EAVKLADRILAIDKSNRVARLVVGVRDLKLKKYAAAQLNINQSIRGPITDLVATLLSG
                     WASYGAGDSKTAIANIDKLTGPEWYPLFKDLHAGMILELAGKEKDAGLRLERAHKLDD
                     SMLRVADDYARWLSRNKDAAAATAVYEAFDKKLPRHPLVLEGLRETKAGKKLSPLVDS
                     PQTGAAEALYGIGATLTRRGGEDLALVYLQLALYLHPNHSLALLSLADLYESVKKPAM
                     AIKVYERMPASSPLKRNAQIQLATNLDAAERSDEAIKILKTVTTEDPKDIEAIMALGN
                     IERGRKKFADCVTTYSQGIDALPQANEKTNWIYYYYRGICEERSKQWSKAEADMRKAL
                     DLQPEQPHVLNYLGYSWIDQGINLDEGMKMIKRAVDQRPDDGYIVDSLGWAYFRIGNF
                     DDAVKNLERAIDLKPEDPTINDHLGDAYWRIGRTLEAKFQWAHARDLKPEPEELPKIE
                     AKIDNGLPDDASSSAASADKKKEDGKGG"
     gene            11303..>11837
                     /locus_tag="VN968_08510"
     CDS             11303..>11837
                     /locus_tag="VN968_08510"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HXL29316.1"
                     /translation="MPALTEDARAKVNLTLRVVGRRVDGYHDLESMVAFADCADRLSL
                     TPGSKLNLIATGPLAQDCGETAENLVFKAAKLLGERVPDLSLGDFTLDKVLPVAAGIG
                     GGSADAAAALRLLAQTNGLAIDDARLIEVAKLTGADVPVCLGSRPCVMTGVGETLLPL
                     SLPRLPCVMVNPRVPVAT"
BASE COUNT         2195 a         3618 c         3755 g         2269 t
ORIGIN      
        1 ccccgcgctg aacgatgccg agatcgagcg cattctcgat gcggcagcgc atgccggggt
       61 caaggaagcg agctatgtgc tgctgcggct gccgctggaa gtgcgtgacc tgttccgcga
      121 atggctgatg gcgaattatc cggaccgcta tcgccacgtc ttcaccatga tccgcgacat
      181 gcgcggcggt cgcgattatg actcgcaatg ggggacgcgg atgaaaggca ccgggccgat
      241 ggcctggatg atcgggcgcc ggttcgagat cgcctgcgaa aagctcgggc tcaacaagcg
      301 gcgttcgaaa ttgaccaccg accatttcgc gcgcccgaag cgcagcgggc agcagttgag
      361 cctgttctag tactgtaaag aagttccggc atgaatcaga aaccacctgc gccggttgcg
      421 cgcctcacca tgatcacgct cggcgtcggc aacatgcgcg ccagtatcgc gttttacgag
      481 gcgctgggtt ttgcgcgaaa atttcgcgca accggcgagg ccgtcgcgtt cttcgaaacc
      541 ggcggcacgg caatcgggct ttttccgtgg catcagctcg cgcaggacgt cacgctgccg
      601 gaagacccgc gcccgcaggc ctttcgcggg gtgacgctgg cctggaattg ccgcagcacg
      661 gaagaggtcg atgcggtgct gaattttgcg atttcgaagg gggcatcgct gttgaagccc
      721 gcgcacaaga ccgattacgg cggctactcc ggatattttg tcgatccgga cggtcatccc
      781 tgggaagtcg tggtcgcacc cagcatcgag gtgggcgagg accggcgcat tcacctgccg
      841 gattgaaccg gcgtgaaccg cggttgcgtt gcggccggca acgccgccgt tacgataaaa
      901 tcttttcggt cacgtcatcg ccggttttgg aatcaacaat tagaaacggc acctgccgtt
      961 cccgccagac ccgcagttca tcgagactgc gatagcgccc gatttcccca tcgtggagcc
     1021 gccggttcgg gtagagcttg atgaggacgg gcaatggttc aagattgctc atttgaacct
     1081 ccagatgaac tcaatcctat catgaaatgc gagggctccc agccgtctgc ctgttgagag
     1141 cggtgaaatg ccggaggcga gtgcgagttc ccagttgcgc cgcaggcttc gcttgcgcac
     1201 gatcccggca tgattcgcga aaaatccgga aacgaccaga ctaggttgcc aaacggcgtg
     1261 attgcaatcg cgccgccgag ctttcgccgc gagcgtgcgc tgatcaagcg cggggtctgg
     1321 ccggtggccg gctgcgacga ggccgggcgc ggcccgctgg cgggcccggt ggtggccgcg
     1381 gcggtcgtgc tcgatcccaa aaggatcccg aagggcatcg acgattccaa acggctgacg
     1441 ccggagcgcc gcgaggaact gttcgaggaa atttgcttga ccgcgtcgtt tgcggtggcc
     1501 tttgcctcgc cggcgcggat cgatcgcgac aatatattgc gtgcgtcgct gtgggcgctg
     1561 gcgcgtgcgg tccacgcgct gccggaaatg cccagacatg tgttcgtcga tggccgcgac
     1621 cggctcgata cggcctgcga ttgcgaagcg gtgatcggcg gcgacgggtt ggtcctttcg
     1681 atcgcggcgg cgtcgatcat cgccaaggtc gcgcgcgacc ggttgatgtg caagctggcc
     1741 caggattgtc cgggctacgg ttttgagtcc cacaagggct atgccgtgcc ggagcacctt
     1801 gaggcgctga cccggcttgg accgacggcg catcaccgcc gcttgttcgc gccggtcgtg
     1861 gcggcccgcg agaaacacga ggcggtcgcg ggccgcccgg tgcggacgca gacggaagct
     1921 ggcatcgagg tctccgccga ggcctgaccg ggggacggtt gggtgtccgg cgatcggcct
     1981 caagccgcct tgagggttgc gcgcagaatt ttcatcagat ccggcatctt gagcggcttg
     2041 gtcaagatat gatcgaagcc tgcgagctcc atgttgtgca aattggaggg catggtatcg
     2101 gcggtcagcg cgacaaccgg aatttgcgag gtccggtcgt ccttgcgaag acccctcagg
     2161 acgtcaaaac ccttgccgtc cggcaggtga atatcggtaa tgacgagatc gggccgcagc
     2221 gagcgcgcta tcctgagccc ttcctccacc gtttgcgcat cgacgaagcg cgtattgctc
     2281 aagtcttcga tgatggcgcg catcatctcg acgttcggaa tcttgtcttc aatataaagt
     2341 atcttgcatc ttgtgtccgc cgcggtcgcg aacagcgacg gtgcacgggc catcttcgcc
     2401 gcgatcgcgc cggccactgg cagctcgacc cagaacttgc tgccccgtcc gaccgagctt
     2461 tcgaagccga tcttgccgtc catcgcttgc accagccgcc gactgattgt cagcccgata
     2521 ccggttcctt ccttctgtgt gagttcggcg ccaggacgat tgaaaggctc gaatatttcg
     2581 ccgtgacgct cggcaggaat tcccttgccg gtatcccgga cgatgaagcg cacgacgtcg
     2641 tcacagggaa acgcggtcag caatacccat ccgccctcga cattgtattt gatcgcattg
     2701 cttccaaggt tcaacagaac ctgaatcagc cgcccgcggt cggcgacaat ggcgggcagg
     2761 tttccggaga catcgaccgt aaagagcacg cctttctttc tcgccgaaac ctccagcgag
     2821 cggctcactt cggtcatgac ttccagacaa tccagaacct cacagttcac gtcgacctcg
     2881 ctggtttcga aacgagccat gtcgaggacg tcgtcaatga gttcttccag ttgggtgctc
     2941 gcttccatga tggactgcgc atagcgaatc cgtttttcgc gctcgagcgt atcgccggac
     3001 cgacctagca attgggcaaa gcccgagatc gcattcagcg gtgtccgcag ctcgtggttc
     3061 atcgatgcga ggaactccga cttcgcgcgg ctggttcggt cggcgatttg ctttgccagg
     3121 atcatttcac gggtggccaa ttcgccttgc tcgcgctcca ccacacgctc cctgatcatt
     3181 cgggaggcct tcatcagcgc ctggccgacg tcgttgactt cgacgatttc gttgtctgga
     3241 atcgacagca attccggtgt tccgagcgca agggccggga cgtgaagcga ccggatcgaa
     3301 cgattgatcc ggacgctgat gactttcgcc atccagatgc tcaaggcgag cagcgcaagg
     3361 gtcacggcgg cgttgaaacc cagtgatcgc cacgggtttc cggtcaggct ggtcctcggg
     3421 atgccgatgg cgacagccca gcccgaccgt ggtgatcggt tgtacgcgct caacacggga
     3481 gtgccctcga gcgagtcggc ttcggtgata cccccggcgc ggttggctga cctcaccagg
     3541 aaggccgccg caccctttcc gcccacaaat cgttcaggga ggtgggttcg agccgcgatc
     3601 gtgccgcggc cgtctaaaat tgccgtgatc caatccagcg gaagattctg ccggcgcagg
     3661 atatcgccaa ggcgatcgcc gaagatgccc atggcgagag agtagatcac cttcccgttg
     3721 gagaagaccg gcacttccag ggtgatcgca gactgcccga catcgggaac aaccaaaaaa
     3781 tcggagattg ccggttttcc gttctcgaac acccgccgca ttttggcgga gggcagcacc
     3841 tgaggcagcg gatcaccgaa tggcttgagt gtattgagac gctgctgccc ggttgaatcg
     3901 gtaatggcga tgtagttgcc tccgatattc ggcaatgcct cgctcgcctg cagataaaaa
     3961 cccgccagat caccggaagc caaacgatcc gatgctgcga gcacctgcag cgccgattgg
     4021 gcaccgaaca gctcggcatc gatcgcctgc atcagcgcgc gggcggtcgt gatcgtgttg
     4081 cgttcgagaa ttgcccgctg ctgctgatag gaaatggtga acaggaacac agatcccagc
     4141 gtcgccggca gaacgcaacc gatgaccaga catgtgagcc aaaatcgaat cgttctctga
     4201 tgcaagtgag attccttgac gcggcctcat caaaggcctc gttatgagaa aaacgcatgg
     4261 caaaacacaa acacagatca atcgggacca cggcggagtc atcccgcctc gtggcgacga
     4321 tcaaccgagg tcgcgcaacg cactattcga cgcttactgt gaaatttacc ggccgttaca
     4381 gggccgccga caggctggat cagccgcatc gcaagccgtc gacgcatctt ccgcaaatca
     4441 atcggctcgc gcgaattgtg agacgcggga aacgcaatgt caattgctcg aaagagtcac
     4501 cgacatttcg agcgcgtgtt cccgcagttg cagaagtggc acatcatgat tgttcgcgac
     4561 ggcgagcaga agatctgtcg acgcgaccga atatgtcacg acagtagctc tttgcagggg
     4621 atcggaagat atcatgaaaa attcggcgcg gcggaagtga agcgcggcac gagaagtcgg
     4681 ccctcggacg ccgcgcattc gccgcgcgga acgaaagtgt ctcgcgaagt gtcacggcta
     4741 aggtgttcgg cctcggagag aaagccggct cacggcagtc acgatcttgc tccgatttca
     4801 gctcacgttg tgccggctgg atgggaagcg agggcgcctg cgcgttgacg cgggatcggt
     4861 ctgttgcctc agcggctcac gcgctttatc acacgttcga gatcgaacag gaccggccat
     4921 aggttcgatc gggcatttcg gacgtttcat gcgctttacc tccctggtca tcgaactgat
     4981 ccgcgcccgg ccgcggctgg tagtctggct tgtggtgctg ctgcaggcgg cgttgtggct
     5041 gatgctgccg ctattgctgt atggcagtcc tcccggcgat cttgcgacgg tactggccta
     5101 tggtcgggaa tatcaggtgg gaaccgatct cggcccgccg ctggcgttct ggctcggcga
     5161 tatcgcgttc cgcgccgccg gcaatcatat gttcggcgtc tatctgctgg cgcagctttg
     5221 cgccgtcgca acgttctgga ccttgtttct cctggcacgc gcggttgtcg gcggacaaca
     5281 ggcggtgctg gcggtcttgc tgacgatgac agtcacggcg ttcggttcgc ctggtgtgga
     5341 gtttggcccc ctggtgctgg cgcgtccgct ctgggcgctg ctgctgctgc attcctggca
     5401 actgatcggg caggggcgcc acaacgcgtg gttcgcatgg tcgatcgaag ccggactctt
     5461 gctgctgacg acctcagccg cgctcggcct gttgattttg gtcgccggat ttgcggtcgc
     5521 aaccttgcgc ggacggcgca cgctgacatc gttcgatccg ctgtacgcgc tgctggtgat
     5581 tgtcgttttg gcgctgccat atctgatctg gctgatccgc gccgatacgc tggcgttgcc
     5641 gccatggccg gcgatcgcgg atttgagcga ccgggcgctg cactggggcg acctgctcgg
     5701 cggcttgctg ttggcaattt ccggcatcgt gctgctggtg gtgatcaact cggcctggtt
     5761 tggccgtaac ccggaggagg cgccgatcat ctatcggccg ccggtcgagc cgctggcccg
     5821 ggatttcgtt tattttttcg ccgtcgcccc cgcgcttgcg ggaagtctga tctccggatt
     5881 tttcaatttc gatcatgtcg cgggcggggc gggcatcgcg ctgttgatgt cgggactggc
     5941 ggtcatcgtg gcgaccggcg acctggttca tctgcggcgt cagcgcgtgc tgcgtctggt
     6001 ctgggccgct gccgtcgtcg cgcctgcgct cgcggtaatc ctgacggcgc tattgttgcc
     6061 ctggaccggc agcgccgagg aggcgacgtc gctgcccgcg accgcgatcg cgcatttttt
     6121 tggcgacagt tttgagcggc gcaccaatcg cccgctgcgc gcggtgaccg gcgacccaca
     6181 gcttgcgagt ttgatcgcat tggacaccgc gcgtccgcat ctgctgcttg acacggcacc
     6241 cgaacgaacg ccttggctga cattggcgaa attcagtgaa accggcggcg tcgtggtctg
     6301 gcgcgcatcc gacacggtcg gcacgccgcc ggccgacatc gcgcagcgat ttcccggcct
     6361 ggtgccggag gtgccgcggg tgttcgagtg gccggtgacc ggccggcagc cgctgctgcg
     6421 gatcggctgg gccatcgtgc ggccgaagac gccgtaagat cgtttgcgag caaggcacgc
     6481 ggtcggtttc ctttacctct cccgtcgaag tcggatttat ccgactttga cgatttgaga
     6541 tacccaactc gggtaaaccc cagggtaaac ccgagttgga tgggagaggg agcgcgcttc
     6601 cactgccgcg tgcagaaccg agcttcagcc ggtggaagaa tgctccagcg cctttgcgat
     6661 cgaacgcaaa tcctgccacg ccatccgctt gtaggacggt gaacgcaaca gatatgccgg
     6721 atggaaggtg gcgagcgcac ggatcatccg cgtgccggtg tcatagtcga accagcgccc
     6781 gcgggttttc atgatgccct cgcgggtcga cagcagggtc tgggtcgagg gattgccgag
     6841 cgtgaccagc acatccggat ttaccagttc gatctgccgc tggatgaacg gcaggcagat
     6901 ttgggtctcc tgcggcgtcg gcgtgcggtt gcccggcggc cgccatggga tgacattggc
     6961 gatgtaggcg ctggagcggt cgagcccgat cgctgcgatc atgcggtcca gcaacttgcc
     7021 ggagcgtccc acgaacggca gcccctcgat gtcctcctca cgcccggggg cttcgccgac
     7081 aaacatgatg cgcgcctgcg ggttaccgtc ggcgaacacc aggcgtgtcg cggtcgattt
     7141 cagcgcgcag ccatcgaaat tctccaacag ggcgcgcagc acctcgagcg aaggtgccgt
     7201 tcgcgccgcc tcccgcgccg acaggatcgc ggcctccggc gctggcgctg cctcgccgcg
     7261 cgccgccggg atcgcggcag gaacggtcct gaccggtttc ggcggcgcgg cttcgcttga
     7321 tagggggatg atgtcgggat cggacagccg gttgaccggc gcttccgtca acgcgcagtc
     7381 gactccggcc tccagataaa aggccagcaa ttgctgaaca gtgggggcat tatcaggcgt
     7441 catggttcca tgctggggca tcaggccaat ctaggatgga ttgcgctgca gcgaaacgcc
     7501 tcaacgcgca tttccatggt tgttctcagg cgaaaaatcg gaaacaaacg acttatcatc
     7561 gctttctcaa ccgggctgag atcagatcat gagcaccgaa gaattgccgc cgcgcgaatc
     7621 catggaattc gacgtcgtga tcgtcggcgc cggaccctcg ggccttgccg ccgcgatccg
     7681 gctcaaagaa ctcaatgccg atttgacggt cgtcgtggtg gagaaggggt cggaagtcgg
     7741 cgcgcatatc ttgtccgggg cggtgatcga tccggtgtcg ctcgacaaac tgattcctga
     7801 ttggcgcgag gacgccgact gtccgctgaa gacccaggtc aaggccgaca agttctactg
     7861 gatgaccacg ggcatggcga ttccgctgcc ggcgatcgcg atgccgccgc tgatgaacaa
     7921 tcaccattgc tatatcggct cgctcggcaa tgtgtgccgc tggctggcgc gcaaggccga
     7981 ggcgctcggc gtcgagattt atccgggctt tgccgccacc gaagtgctgt acgatgaaaa
     8041 gggcgcggtg cgcggcatcg ccaccggcga catgggcgtc gccaaggacg gctcgcacaa
     8101 atcgtcgtac acgcgcggca tggagctggt cggtaaatac acgctgttcg ccgagggcgc
     8161 gcgcggcagc ctgaccaaac agctgatcgc aagattttcc ctcgatgccg gcagcgagcc
     8221 gccgaaattc gggatcggcc tcaaggaggt ctggcagatc gaccccgcca ggcatcaaaa
     8281 aggtctgatc cagcattcgt tcggctggcc gttggccaat tcgaccggcg gcggctcgtt
     8341 cctgtaccac tacgacgaca atctggtggc ggtcggtttt gtcgtgcatc tcaactacga
     8401 cgatccctat ctgtcgccgt tcgatgaatt ccagcgcttc aagacccatc ccgcgatccg
     8461 caccttattc gaaggcggca agcggctcgc ttatggcgcg cgcgccatca ccgagggcgg
     8521 ttatcaatcg gtgccgcgcc tgagctttaa gggcggcgcg ctgatcggct gcgcggcggg
     8581 ctttgtgaat gtgccgcgca tcaagggcgt ccacaatgct gttggcagtg gcatgctggc
     8641 tgcccaacac gtcaatgccg cgctgggctc gggccgcgcc aacgatgaac tggtcgatta
     8701 tgagaatgcc tggcgcgatt cgcccgtcgg caaggacctg tacccggtcc gcaacgtcaa
     8761 gccgctatgg tcgaagttcg gcaccgttgt cggcgtggca ctcggcggca tcgacatgtg
     8821 gtgcaacacg ctcggcttct cgctgttcgg aacccagtcg catgccaagc ccgaccgcaa
     8881 gacgctcgat ccggcgaaag cccatgcgcc gatcgcgccc ccgaaaccgg acggcaagct
     8941 gaccttcgac aagctttcct cggtgttcct gtccaacacc aaccatgaag aggaccagcc
     9001 ggttcatctc aaggtcgccg acatgaacct gcagaagaca tccgaacatg acgtctatgc
     9061 cggtccgtcc aatcgttact gtccggccag cgtctatgaa tgggtggagg agagcgccgg
     9121 gccacgcttt cagatcaacg cccagaattg cgtccattgc aaaacctgcg acgtgaagga
     9181 cccgaacggc aatatcacct gggttccgcc ggagggcggg ggtggaccga attatgaggc
     9241 gatgtaacca cgattgcgtg agccggcacg cgccaatgcg gcccccggcc acgataccgc
     9301 cacactgcaa gcgttttcag gcggccccgg cggccatcga gctttgctgc caatcgaatc
     9361 gccacccgta tccttgcggt tgagcgccaa acgcggcatt gtcacacggc tggatgccgc
     9421 ccgttgggtt cgcatcgaga atcggttggt gatgctgccg taaattcctg aaagccaagg
     9481 cgaaccctga tgttttccat tcgtttcaat cgctggacga tcgccgcagt cgccttcacc
     9541 gtgctggccg tgccggcttc ggtttcggct cagacgcctg atcatccggc cgacaactcg
     9601 gcgcaatttc cctctcgcgg cgatctgaaa gcgctgacca cgtccggcag ctatctggcc
     9661 gcccgtcatg ccagcgtcga gcgggacgcc agctcggcgg cggcgtttta ccgctcggcg
     9721 cttcgcaccg atccgaaaaa caacgaatta atggatcggg cgttcatttc atcgctcgcg
     9781 gatggcgaca ttgacgaggc cgtcaagctc gccgatcgaa tcctggcgat cgacaaatcc
     9841 aatcgcgtgg cccggctggt ggtcggcgtg cgcgatctga aactgaagaa atacgcggcc
     9901 gcgcagttga acatcaatca gtcgatacgc ggaccgatca cggatctggt ggcgacgctg
     9961 ctgtcgggct gggccagtta cggcgccggc gattccaaga cagccattgc caacatcgac
    10021 aagctgacgg gcccggaatg gtacccgctg ttcaaggacc tgcacgccgg catgatcctc
    10081 gaacttgcgg gcaaggaaaa ggacgccggc ctgcgcctcg agcgggccca caagctcgac
    10141 gattcgatgc tgcgcgtggc cgacgactat gcgcgctggc tgtcgcgcaa caaggatgcg
    10201 gcggcggcga ccgccgtcta cgaggcgttc gacaagaagc tgccgcggca tcccttggta
    10261 ttggaaggcc tgcgcgagac caaggccggc aagaagctgt caccgctggt cgattcgccg
    10321 cagaccggcg ccgccgaggc gctgtacggc atcggcgcga cgctgacgcg ccgcggcggc
    10381 gaggatctcg cgctggtcta tctgcagctc gcgctgtacc tgcaccccaa tcattcgctg
    10441 gcgctgttgt cgctggccga cctctatgag tcggtgaaga agccggcgat ggcgatcaag
    10501 gtctatgagc gcatgccggc gagttcgccg ctgaagcgca acgcgcagat tcaacttgcg
    10561 accaacctcg atgctgccga gcgcagcgat gaagccatca agatcctgaa aaccgtcacg
    10621 acggaagatc ccaaggatat cgaagccatc atggcgctcg gcaatatcga gcggggccgc
    10681 aagaagttcg ccgactgcgt cacgacctac tcgcagggca tcgatgcact gccgcaggcc
    10741 aatgaaaaga ccaactggat ctattactat tatcgcggca tctgcgagga gcgctccaag
    10801 cagtggagca aggccgaggc cgatatgcgc aaggcgctcg atctgcagcc cgagcaaccg
    10861 catgtgctga actatctcgg ctattcgtgg atcgatcagg gcatcaatct tgacgagggc
    10921 atgaagatga tcaagcgcgc ggtcgatcag cgccccgacg acggctacat cgtcgactcg
    10981 ctgggctggg cctatttccg catcggcaat ttcgacgacg cggtgaaaaa tctcgagcgc
    11041 gcgatcgatc tcaagcccga agacccgacg atcaacgacc atctcggcga cgcctattgg
    11101 cgcatcggcc gcacactgga ggcgaaattc cagtgggccc atgcccgcga cctgaaaccg
    11161 gagccggaag aactgccgaa gatcgaggcc aagatcgaca acggcttgcc cgacgatgct
    11221 tcttcctccg cagcctccgc ggacaagaag aaagaagacg gcaagggcgg ttgagggcgg
    11281 ctgttcgaag ggctgctcgc cgatgccggc gctaaccgaa gatgcgcgtg ccaaggtcaa
    11341 cctgaccctt cgggtggttg gacgccgggt cgatggctat cacgatcttg aaagcatggt
    11401 tgcgttcgcc gattgcgccg accggctcag cctgacgccc ggatccaaac tcaatctcat
    11461 agccaccggg ccactggcgc aggactgcgg cgagaccgcc gaaaacctgg tgttcaaggc
    11521 cgcgaaattg cttggcgaac gcgtgcccga tctcagcctt ggcgatttca ccctcgacaa
    11581 ggtgctgccg gtcgcagcgg gcatcggcgg cggctcggcc gatgccgccg cggcgctgcg
    11641 attgctggcg cagaccaatg gtcttgcgat cgacgacgcg cgcctgatcg aggtcgcgaa
    11701 attgaccggc gccgacgtgc cggtctgtct gggctcgcgt ccctgcgtca tgaccggcgt
    11761 cggtgaaacc ctgctgccgc tgagcttgcc gaggctgccc tgcgtgatgg tcaatccgcg
    11821 ggttccggtg gcgaccc
//
LOCUS       DATNOI010000222         2985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1836608,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000222 DATNOI010000000
VERSION     DATNOI010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836608"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="VN968_08515"
     CDS             complement(<1..1168)
                     /locus_tag="VN968_08515"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: protein motif:HMM:NF017873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HXL29317.1"
                     /translation="MRAHIIGGGFGGLAAAALLIRNAGVSGEDVTIYEADEQVGGGFF
                     LGGSAESGYNLPGSVFDKEFRCTFELLKSIPSASNPSISVTEEFFAFNTGEPYHDGAH
                     IFDRDGRVVHGPRFGLSLGDGLSLARVLLTPEAMLNGRRIVEFFSRRFFSTEFWFLWS
                     TIMGSLPEHSAIEFRRYMNRFLYLFGHLSDMTGVMRTPINQHQAFIEPLVAWLHPRGV
                     NFLTAAFVQDIGFAPSPGRMTVDRLDYERGGSVTSVAVAPEDLVLVTTGSQAADLSVG
                     SMTEPPRPRPSGRSWALWTRLAKGSPKFGKPDAFFGEARMPLSRWVTFTVTDRGTEFI
                     ERISALTGSELGRGGLVTLKDSGWVLSLSIFHQPEIIGQPPGTSVWWGYGLYPER"
     gene            complement(1969..2946)
                     /locus_tag="VN968_08520"
     CDS             complement(1969..2946)
                     /locus_tag="VN968_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXL29318.1"
                     /translation="MRARVSRETGRAVEVISCYEAGYDGFWLHRQLEAHGIRNYVIDP
                     ASLQVDRRARRVKTDRIDTERLLRSLMAYLRGEPKVWSVVRVPSVAEEDARRLHRERD
                     RLVSERVQHVNRIKGLCALQGIYDYNPLRPQAMAGLEQLRTTQGGALPPRLKSEIKRE
                     LQRLELVVEMIATLEAERNAIVEDEASTHVNAKKIQNLHKLKAIGPEFAAVLVGEIFH
                     RDFNNRRQLASYVGFAPSPFQSGNVAHDQGISKAGNRKGRTTGIELAWLWLRYQPDSD
                     LSVWFRTRVGTTKGRIRRIAIVALARKLLVALWRYLETGVVPRGAVLKG"
BASE COUNT          561 a          978 c          871 g          575 t
ORIGIN      
        1 tgcgctcggg atacagaccg tagccccacc agacgctcgt gcccggcggc tggccgatga
       61 tttctggctg gtggaagatg gacagcgaca gaacccagcc cgagtccttc agagttacga
      121 gcccgccgcg tcccagctcg ctgccggtca gcgcggagat tcgctcgatg aattcggtcc
      181 ctctgtcggt gaccgtgaaa gtcacccagc gcgacaaggg catccgggcc tccccgaaga
      241 atgcgtcggg ttttccgaat ttcgggctcc ccttggcgag gcgcgtccat agcgcccagg
      301 accgtccgct cggccgcgga cgcggcggct cggtcatcga tcccaccgaa aggtcggccg
      361 cttgcgatcc ggtggtcacg agcacgagat cctccggcgc gaccgcgacc gaagtcacgc
      421 tgccgccccg ttcatagtcg agtcgatcga cagtcatgcg gccgggcgac ggcgcgaaac
      481 cgatgtcctg cacgaaggca gcggtgagga agttcacgcc gcgcgggtgc agccacgcga
      541 ctagcggttc gatgaacgct tggtgctgat tgatgggcgt gcgcatcacg cccgtcatgt
      601 ccgacaggtg accgaacaag tacaggaagc ggttcatgta gcgccgaaac tcgattgcgc
      661 tgtgctcggg cagcgacccc atgatcgtcg accagaggaa ccagaattcg gtcgagaaaa
      721 atcgccgaga aaagaactcc acgatgcgcc gcccgttcag catcgcttcg ggcgtcagca
      781 gaacccgcgc aaggctgagg ccgtcaccca ggctcaggcc gaaacgtggg ccatgcacga
      841 cgcggccgtc gcgatcgaag atgtgcgccc cgtcatggta cggctcgcca gtattgaacg
      901 cgaagaattc ctccgtgacg gaaatagagg gattgcttgc ggagggaatc gacttgagca
      961 gttcgaacgt gcagcggaat tccttgtcga acactgagcc aggcaggttg tagccactct
     1021 ccgcgctgcc gccgagaaag aacccgccgc ccacctgctc gtccgcctcg tagatcgtga
     1081 cgtcctcgcc ggacaccccc gcgttccgga tgagaagcgc ggccgctgcc agtccgccaa
     1141 atcctccacc gatgatgtgc gccctcatcg gctttctccc cggactctgc ggcggcgctt
     1201 tgaaaccgac aacccacatc aactgccgcc ggtcgctgca agcgaccatg ctgctacggc
     1261 accgccgagc agcgtgataa actcgcgccg cgtaatgccg agcatgggca ttcctacgag
     1321 cagacgcgtt gcggagcaag gggtgccgtc ggttcccctc ctaggctcga ctagaggata
     1381 gcaccattag ccggtggcaa atagagtgct gcactgcgga atccaggtcg cttccgtcgc
     1441 ctgaattatg gtcgcggtag ggacgctcat tactgagcgc ccccgcacag atcctggcgt
     1501 gcgctgctaa cgcaccgggc ttatatgagg acgcggctgt caaggggata ttccggtggt
     1561 gtttagcgat cttgatagct cttggttacg gatttaggcg cgcggtcaag ccccgcagcc
     1621 gcaaagcggt gcgccgaagg cgcagggctt gagggcgcgc ctaaatccgt aacatcatcg
     1681 aagagatcgc aaatactttc ctactccagc actcctgcgt ttctttgagc tatggtttcg
     1741 ttccgatcca aggcgagccc tttgcgtgcg tgcggcactc gccgcaccaa cttatatccg
     1801 gccgggttca acccgttcca aaatccctca tgcgcattgc gagggcgcgg gctccaaggg
     1861 ggatgggacc tttctcttcc gcggcatctt tcagccctag cccggtgccg gtcacacatc
     1921 caccttggat ctcataagct tctgttatcg gtatttggtc ttcgttcgtc accctttgag
     1981 gacggcacct cgcggcacca cgccagtttc aagataacgc cagagcgcca ccagcagctt
     2041 gcgcgccaac gccacgatgg cgatgcggcg gatgcggcct ttcgtggtac cgacacgcgt
     2101 ccggaaccag acgctcaaat cgctgtcggg ttgatagcgc agccacagcc aggcgagttc
     2161 gatcccggtg gttcgtccct tgcggttgcc agccttgctg atcccttggt catgcgccac
     2221 gtttccgctc tggaacgggc tcggcgcgaa gccgacataa cttgccagtt ggcggcgatt
     2281 gttgaagtca cgatggaata tctcgccaac cagcaccgcg gcaaactccg gaccgatcgc
     2341 cttgagctta tggagatttt ggattttctt tgcgttgaca tgcgtcgatg cttcatcctc
     2401 aacaatcgcg ttgcgctccg cttcaagagt ggcgatcatc tcaaccacga gctccagacg
     2461 ctgcaattcc cgcttgatct ctgattttag ccgcggcggt aacgcgccgc cttgcgtcgt
     2521 gcgcaattgc tccagccccg ccatcgcctg cgggcgcaac ggattgtaat cgtaaatgcc
     2581 ttggagcgcg caaagcccct tgatacggtt gacgtgctga acgcgttccg agactaaacg
     2641 gtcccgctcg cggtgcagcc gccgcgcatc ctcttcggca acgctgggca cccgcaccac
     2701 actccacacc ttcggctcgc cgcgcagata cgccatcagc gagcgcagca accgctcagt
     2761 gtcgatccga tccgtcttca ctcgtcgcgc ccgccggtcc acctgcaggc tcgccgggtc
     2821 aatgacgtaa ttgcgtatgc cgtgcgcttc gagttgccgg tgcagccaga aaccatcgta
     2881 gccggcctcg tagcacgaga tcacttcgac cgcccgaccc gtctcgcggc tgacacgtgc
     2941 ccgcacctcc tcgatcaact cgagtagcct tttccatttg catcc
//
LOCUS       DATNOI010000223         8050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1471128,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000223 DATNOI010000000
VERSION     DATNOI010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471128"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1101)
                     /locus_tag="VN968_08525"
     CDS             complement(124..1101)
                     /locus_tag="VN968_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HXL29319.1"
                     /translation="MTPLRLVRAALLLLMAILPHELCAHEATLGVLEFREVRPGAFVG
                     RWTMDPGIGAARVDLRVPPHCFLRLPEMNCGERGLVGPITITNLGADMSAVLIKIIPI
                     ADQPRSYTISTANPVVSVLGTGAPTLQTWIELARTYVNYGIDHILLGADHLLFVLGLI
                     WIVGGGWRLVKTITAFTIGHSASLAAAAFGLIGVPERPLNACIALSIVFVGVEIAKQQ
                     RGEAGLTARYPWAVAFTFGLVHGIGFASALAGLGLERRLLPAALLFFNIGVEIGQLAF
                     VLLVLALIWGHQRLDAVLPRWGSVLPAYAIGAVSMFWFLGRLLRVLSTT"
     gene            complement(1098..1907)
                     /locus_tag="VN968_08530"
     CDS             complement(1098..1907)
                     /locus_tag="VN968_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXL29320.1"
                     /translation="MRPIAAGTARFLRREPLVHFVLLGALIFGADAVLHPPPKDDKVI
                     TVTKAMRQSFIENFDEDKSRTPSDAEMEKMIESWVASEILYREGKALGVDRGDDMIRD
                     RVAFKLQLLIFDQIRVPQPTEDQLRAWFADNHGRFDEQERVGFYLTPPTDEITAQRQL
                     ADIEGQHESEDLQRQTHAILGRPVASLAASFGESFRDALLALPQGQWKALQSKEGWHV
                     VRLDSRRAAVLASLDNVRDEAVRLWLTDETRKRAWEAVSRLKAAYTVRYEP"
     gene            2314..4338
                     /locus_tag="VN968_08535"
     CDS             2314..4338
                     /locus_tag="VN968_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29321.1"
                     /translation="MFPSAKTILLTAASVSLALTHTATANDRDGDKPRANPYLAGDMH
                     NHNTCTDGSVSAGYAIDRSVAAGVAAGGGTNFDLDWFTLGNHGGSGNRDCRFSDTSAN
                     LPGDPTTTWSQTLGQTIDGITITSLKGTPNGTGSGATMWRWQSIKEVEYPIIVNRTPI
                     YNKVLVEGLEWIVPGHEHTDVAVIAGQNPRGFGSADKMAEYEFRFDRADTDAIGLLDA
                     SNKQVWTGKDNVNNSGTAGHQKAVSGVTWLQTHFPLQSYAIPTHSERAGAFNPASSAG
                     YNIEHFRDFNNAGPTVAFGIESPGHMAQGSLTGGSGSYTPNAVGGGTYGFSGVYTAKV
                     GGLWDGLLGEGRNVFIYASSDWHNRGAGGARDSFTTSDFIPGEFTKLYLPNTEHFKAQ
                     SIIDGMRSGNSYSVNADIIGPDMVFRARGRDDDWKTMGETLVVRPGEKITVEMSMTVP
                     AKNNSPYSFNNPLLQQVGIQQPLNKPSLDHVDLITGKITGVVPPGSPNYAVPNVSGVA
                     GAAIAYNPTTTISQQIHANNMRSTRLRDGSTKLTFTTTFTAGSTPSYIRARGTNIPVA
                     TPNVTDSAGSPILDINNSLVSCTDPACPTHLATLNGVKKVTFDVQAYSNVWFYANPIF
                     IRPEGSAKLLVEQNADLAKRLAERADNDHDDHDRHDHDDRDHDGRDHDRD"
     gene            4563..4778
                     /locus_tag="VN968_08540"
     CDS             4563..4778
                     /locus_tag="VN968_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29322.1"
                     /translation="MAQTRALAAALPADDLSRYFAERDLHQAIVEAGNGEFDDCLELA
                     AHATDEIKERRHVLQPGERLKVLKSNE"
     gene            4926..5549
                     /locus_tag="VN968_08545"
     CDS             4926..5549
                     /locus_tag="VN968_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HXL29323.1"
                     /translation="MDQIEPLFPIPLLRSPRLLGPDLNAAAVAAIRGARIESNLRSDQ
                     LFHTEVADPRDNKLFREIADLVVPKLVDFGVLLFGEKLRWTVKEMWTNMLEKGGSQTL
                     HSHANSFVSGIIYLTPSHPACRTVFMRPLGGSDFSFRHHTRSAAMGPFNAGKYVLPEA
                     ELGDLVLFPSYLYHEVPRNQGEQRITVSFNAIPDSLDCWGYRINFAP"
     gene            complement(5579..>8050)
                     /locus_tag="VN968_08550"
     CDS             complement(5579..>8050)
                     /locus_tag="VN968_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HXL29324.1"
                     /translation="EIVWTRQLSLALGSEMMAVLGAVAGFFAGLALGAFALDRVIRRT
                     RSPRTAYIALEAVIGLWGLICIWLLPAAGRALSPLLGTEPAPALLWAASFALPTLVLL
                     PATAAMGGTLTALERLVREACGDGRVSAGVYGANTFGAVAGTLTSTFLLLPALGLSGT
                     LVCLASVNAVCAAGVLALGSAAGPAPMEASAAEPRIIGGSRLTITLLATGLLGIAFEV
                     LVVRLAAQVMQDTIYTFAGLLAAYLLGTAAGGLAWQRAGRRVQDASLGWLLAGTALAC
                     LTTALLTPYIAGIAETAVEGGLAGELAVAMALFLVPSAAMGALFGLLAQRTRDERGSL
                     GWAVGINSIGASMAPLLTAQFLIPGLGAWRALIPVALGYLLLVPLRRAALVWCAAPAL
                     VAIVLLVRPAPSLTRVPAGGALLAVREGPMVTASVVDDASGARYLEVNGRFRMGGTSS
                     ARSDYRQAMLPLLLHPAPHRALFLGIGTGATLIGGSQMPGVTVQGVELSREVVELLPW
                     FAPSGATAAAPRVTVADARRYVAADTTQYDVIIADLFHPSLDGSGALYTTDHFEAVRR
                     RLAPGGVFCQWLPLYQLDLPSLRAIIRGFLEIYPGGSAWLNHYSVRTPMLALIGPRDG
                     GALDLDALDARLRDPAVRPVARRLGLETSMDLLGQYLGGPRVLSAFAGQGPRNTDDYP
                     FVTFDARRNVQALTAPPWTLLRTVTQALRPDPAELVAEPMRDALSERLTAYWRARNRF
                     LDAGAALPGDPRGAALIETASPGLLDALRLSSEFDPAYGPLIGMARSLMTSDRAAAAR
                     LLHAINDAAPSRGEAREMLSREFGQ"
BASE COUNT         1612 a         2634 c         2480 g         1324 t
ORIGIN      
        1 cggcgagacc gccggccacg ccgacttgct cgtgcgcgaa caccggttgc gccagcgcga
       61 gaactgcaag cgcgaccaca agacgcggaa aaacgcgaaa ggacatggtt cgaccgcttc
      121 ctgtcatgtg gtggaaagca cgcgcaagag acggcctaaa aaccagaaca tcgacactgc
      181 gccgatcgcg taggccggta agacactgcc ccagcgcggc agaacggcgt cgagacgctg
      241 gtgcccccag atcagggcca gcaccaaaag cacgaatgcg agttggccga tctccacgcc
      301 gatattgaag aacagcaggg cagcaggcag caggcgacgt tccagaccca gtccagccaa
      361 tgcgctcgcg aagccgatgc cgtgaaccag gccgaaggtg aatgcgacgg cccagggata
      421 gcgggcggtc aggccggctt ctccgcgctg ctgtttcgcg atctcgacgc ccacgaacac
      481 gatgctcagc gcgatgcagg cgttgagcgg gcgttccggg acgccgatca gcccgaaggc
      541 cgcggcggcg agcgaggcgc tgtgtccgat cgtgaaggct gtgatcgtct tgaccagccg
      601 ccacccgccg cccacgatcc agatcaaacc cagcacgaac aacagatggt cggcgcccag
      661 caggatgtgg tcgatcccgt aattcacata ggtccgggcc agttcgatcc aggtctgcag
      721 tgtcggagcg ccggtgccga gcactgagac gacggggttc gcggtcgaga tcgtatagct
      781 gcgcggctgg tccgcgatcg gaatgatctt gatcagcacc gccgacatgt cggcaccgag
      841 attggtgatc gtgattggcc cgaccagtcc tctctcgccg caattcatct ccggcaggcg
      901 cagaaaacag tgcggcggca ctcgcaggtc gacacgcgcg gcgccgatgc cgggatccat
      961 ggtccagcgg ccaacgaagg ccccgggtcg aacctcacgg aattcgagca cgcccagcgt
     1021 ggcttcgtgc gcgcaaagct catgcggaag gatggccatg aggagcagca gggctgcccg
     1081 caccagtcgg agcggcgtca tggctcgtac cgcacggtat aggccgcctt caggcggctc
     1141 accgcctccc aggcgcgctt gcgggtctcg tcggtcagcc agagccggac ggcttcgtcc
     1201 cgaacgttat cgagactggc gagcacggcc gcgcgccggg aatccagtcg aacgacgtgc
     1261 cagccttcct tggactggag tgctttccat tgaccctgcg gcaacgccag aagcgcatcg
     1321 cggaagcttt cgccaaagga tgcggccagg cttgcgaccg gacgtccgag aatggcgtgc
     1381 gtctgcctct ggagatcctc cgattcgtgc tgcccctcga tatccgcgag ctggcgctgc
     1441 gccgtgatct cgtcggtcgg cggcgtcagg taaaacccga cacgctcctg ctcgtcgaac
     1501 cgcccgtgat tgtccgcaaa ccacgcgcgc agttgatctt ccgtcggctg cggaacgcgg
     1561 atctggtcga agatcagcaa ctgcagcttg aaggcgacgc ggtcgcggat catgtcgtcg
     1621 ccccggtcga cgccaagcgc cttgccctcg cggtacagaa tctcgctcgc gacccagctc
     1681 tcgatcatct tttccatttc ggcgtcggaa ggtgtgcgcg atttgtcttc gtcgaaattt
     1741 tcgatgaagg attggcgcat cgccttggtc accgtgatga ccttgtcatc cttcggcggc
     1801 ggatgcagaa ccgcatcggc accaaagatc agcgccccga gcagaacgaa atgcaccagc
     1861 ggttcgcgcc gcagaaagcg cgcggtgcca gccgcgatcg gtcgcaacga atctgccagg
     1921 cgatttgccg gatggacggc gctgcggccg gaattttcgt tggatgatac aagaagggtt
     1981 tgatcggtcg gtagcactgg caatgtcata tcttgttcac aaatctctca cggcatggcg
     2041 tacgcgcaag gctcgtgcgc gctggcttat gcaacggcgc agtcagtctg tcacttgcca
     2101 atcgggcagc tcttgaaaaa aatcgaaacc gaccagccgg atgtctgatc tggttttttc
     2161 cggggactgt tctttccaga aataaaacct tcaccaaact gtctccgaat tctgattttc
     2221 gtgggccata gagaaaattc gcgcatcgcg caccgcgagt gaacttcgca ttttcaattg
     2281 gcgcgtatcg agcatcgcta agggggcgat catatgtttc catctgccaa gacaattttg
     2341 ctgaccgcgg cttcggtctc gctggctttg acgcataccg ctacggccaa cgaccgggac
     2401 ggtgacaagc ccagggccaa cccatacctc gccggcgaca tgcacaatca caatacctgc
     2461 accgacggtt ctgtttcggc cggctacgcg atcgatcgtt cggttgcggc tggcgtcgcc
     2521 gccggcggcg gcaccaattt cgatcttgat tggttcaccc tcggcaacca tggcggttca
     2581 ggcaaccgcg actgccgttt cagcgacacc agcgccaacc tgccgggcga tccaacgacc
     2641 acctggagtc agacgctcgg ccaaaccatc gacggcatca caataaccag cctgaagggc
     2701 acgccgaacg gcacgggcag cggcgccacc atgtggcgtt ggcagtcgat caaggaagtc
     2761 gaatatccga tcatcgtcaa ccgcacgccg atctacaaca aggtgctggt cgaagggctg
     2821 gaatggatcg ttcccggcca cgagcatacc gacgtcgctg tcatcgccgg acagaatccg
     2881 cgcggtttcg gcagcgccga caagatggcg gagtacgaat tccgctttga ccgcgccgat
     2941 accgacgcga tcggcctgct cgatgccagc aacaagcagg tctggaccgg caaggacaac
     3001 gtcaacaaca gcggtacggc aggccatcaa aaggcggtgt cgggcgtaac ctggttgcag
     3061 acgcattttc cgctgcaatc ctacgccatc ccgacccaca gcgaacgcgc gggcgcattc
     3121 aatcccgcct ccagcgccgg ttacaacatc gagcatttcc gcgatttcaa caatgcggga
     3181 ccgaccgtcg ccttcggcat cgaatccccg ggtcacatgg cccagggatc tctcaccggc
     3241 ggctcgggct cctacacgcc caatgcggtc ggcggcggca cctatgggtt cagcggcgtc
     3301 tatacggcga aggtcggcgg gctgtgggac ggcctgctcg gcgaaggccg caatgtcttc
     3361 atctatgcca gttccgactg gcacaatcgc ggggcgggtg gcgcgcggga ctcgtttacg
     3421 accagtgact tcatcccggg cgaattcacc aagctctatc tcccgaacac cgaacacttc
     3481 aaggcgcagt cgatcatcga cggcatgcgt tcgggcaact cgtattcggt caatgccgac
     3541 atcatcgggc cggatatggt gttccgcgcc aggggacgcg acgacgactg gaagaccatg
     3601 ggcgaaacgc tggtggtaag gcctggcgaa aaaatcacgg tcgagatgtc catgaccgtt
     3661 cctgccaaga acaacagccc ctacagcttc aacaatccgt tgttgcagca ggtcgggatc
     3721 cagcagccgc tgaacaagcc gtcgcttgac cacgtcgacc tcatcaccgg aaaaatcacc
     3781 ggtgtggtcc cgcccggcag cccgaactac gcagtgccga atgtcagcgg cgtcgccggt
     3841 gcggcgatcg cgtacaaccc gacaacgacg atttcgcagc agatccacgc caacaatatg
     3901 aggagcacac ggctgaggga cggctccacc aagctgacct tcacgaccac cttcaccgcg
     3961 ggctcgacac cgagctatat ccgcgcccgc ggaaccaaca ttccggtcgc aacgccgaat
     4021 gtcaccgaca gcgccggcag cccgatcctc gatatcaaca actctctggt aagctgcacg
     4081 gatcccgctt gcccgacgca tttggcaacc ctcaacgggg tcaagaaggt gaccttcgac
     4141 gtgcaggcct actcgaacgt ctggttctac gccaacccga tcttcatccg gccggaaggt
     4201 tcggcgaagc tgctggtgga acagaacgcc gacctcgcaa agaggcttgc ggagcgagcc
     4261 gataacgatc acgacgatca tgatcggcac gaccacgacg atcgtgatca cgacgggcgc
     4321 gatcacgacc gcgattgagg tcagtaagtg tggcggtgca ggattttccc tgcaccgcca
     4381 actcttcatt cccacgcagg atacccgccg cgcgtccgcc ggcttgttgt ggaccaatgc
     4441 atctacgtcg aatgaatgcc ttaactgttt gccttctcgc ctgcatcgcg tgcggcgcgg
     4501 ccgggccgtg tagcggccaa tccgcttcgg ccgagccaac cagggagggc tgcgaggcgg
     4561 cggtggcgca gacgcgtgct ctggcggcgg cccttcctgc tgacgatctc tcccgttatt
     4621 ttgccgagcg cgacctgcac caggccatag tcgaagccgg caacggcgag ttcgacgatt
     4681 gcctggaatt ggctgcgcac gcgacggacg aaataaagga gcggcgccat gtgttgcagc
     4741 ccggcgagag gttgaaagtc ctgaaatcca acgaatagtc gctgcggcgg cggccttcgc
     4801 cgcgcggacg ccatcgataa aatctggcag aatcgatcgc tgtcacctga ttgttttacg
     4861 gctgttgcag tatgcccttc gcgcgggcat tgctcgcatt tcccagtcac cgtggacctt
     4921 gagacatgga tcagatcgag ccgcttttcc cgatccctct gctgcgttcg ccgcggctgc
     4981 tcggtcctga tctcaacgcg gcggcggtgg cggcgatccg cggcgcgcgg atcgagagca
     5041 atctgcgctc tgatcagctc ttccataccg aggtcgccga tccgcgcgac aacaagctgt
     5101 ttcgggagat tgctgacctg gtcgtcccga agctggtcga tttcggggtt ctcctgttcg
     5161 gtgaaaagct gcgctggacc gtcaaggaga tgtggaccaa catgctcgag aagggaggaa
     5221 gccagacgct gcactcgcat gccaatagtt tcgtctcagg catcatttac ctgacgcctt
     5281 cgcatccggc gtgcaggacg gtgttcatgc ggccgctcgg tggttctgat ttttcctttc
     5341 gccatcatac ccgaagtgcc gcgatgggcc ctttcaacgc gggcaagtac gtattgcccg
     5401 aggccgaact gggagatctc gtgctgtttc ccagttacct ctatcacgaa gtgcccagga
     5461 atcagggcga gcagaggatc acggtctcct tcaacgccat tcccgacagc ctggattgct
     5521 ggggctacag gatcaacttc gcgccctgag ccgaattgcg ctggggcggc ctgcatggtc
     5581 attggccgaa ttcccgcgac aacatctccc gtgcttcgcc acgtgacggc gccgcgtcgt
     5641 tgatcgcgtg caacaagcgc gcagccgccg cccggtcgga cgtcatcagt gaccgcgcca
     5701 tgccgatcag cggtccatag gcggggtcga actcggaact gagacggagc gcatcgagca
     5761 acccaggaga cgccgtctcg atcagagcgg cgccgcgcgg atcgcccggc agtgccgcgc
     5821 ccgcatccag aaagcgattg cgcgcgcgcc aataggcggt caggcgctcg ctcagcgcgt
     5881 cgcgcattgg ctccgccacg agctccgccg gatcgggtcg caatgcctgc gtgacggtgc
     5941 gcaggagggt ccatggcggc gccgtcagcg cctgaacatt gcgcctcgca tcgaacgtca
     6001 cgaagggata gtcgtcggtg ttgcgcggcc cctggccggc aaaggcggaa aggacgcgcg
     6061 gtcctcccag atattgcccg agcaagtcca tcgatgtctc aaggcccaac cgccgcgcga
     6121 caggcctgac cgccggatcg cgaagccgcg catccagcgc gtccagatca agggcgccac
     6181 catcgcgagg gccgatcaaa gcgagcatcg gcgtgcggac gctgtaatgg ttgagccagg
     6241 ccgaaccgcc cggatagatc tccagaaatc cgcggatgat cgcgcgcagc gatggcagat
     6301 caagctgata caagggcagc cactggcaaa acaccccgcc tggagccaag cgtcttcgca
     6361 cggcctcgaa atggtcggta gtgtagagag cgccgcttcc gtccagcgac ggatggaaca
     6421 ggtcggcgat gatcacgtcg tactgagtag tgtccgcggc gacgtatcgg cgcgcatcgg
     6481 cgactgtgac ccgaggcgcc gcagcggtcg caccggaagg cgcaaaccag ggtaacagct
     6541 caaccacctc gcgcgatagt tcgacgcctt gcacggtcac gcccggcatc tgcgatccac
     6601 cgatcaaggt ggccccggtt ccgatgccca ggaatagcgc ccggtgcggc gccggatgca
     6661 gcaacagcgg cagcatcgcc tgccgatagt cggaccgcgc cgaacttgtg ccgcccatgc
     6721 gaaagcggcc gttgacctcc aggtagcgcg cgccggaagc gtcatcgaca acgcttgccg
     6781 tcaccattgg cccttcgcgg acggccagca gcgcgccgcc tgcaggcacc cttgtcagag
     6841 agggggcagg gcgcaccaac agcacaatag cgacgagcgc cggtgccgcg caccagacca
     6901 gcgcagctct gcgcagcggt accaacagca ggtaacccag cgccaccgga atcagcgccc
     6961 gccatgcacc aaggcctgga atcaagaatt gcgccgtcag cagcggtgcc attgaagcgc
     7021 cgatgctgtt gatgccgacc gcccatccga gcgagccacg ctcatcgcgc gtccgctgcg
     7081 ccagaagacc gaacagtgct cccatggcgg ccgacggcac gagaaacaac gccattgcga
     7141 cagccagttc tccggcgagg cctccctcga ccgcggtttc ggcgatgcct gcgatatagg
     7201 gcgtaagcaa cgccgtcgtc agacaggcga gggcggtccc tgcgagcagc cagcccaggc
     7261 tcgcatcctg gacccggcgt ccggctcgct gccacgcaag gcccccggcc gcggtgccga
     7321 gcagataggc tgccagcaac cccgcaaagg tgtagatcgt gtcctgcatc acctgcgccg
     7381 cgagcctcac gaccaggacc tcgaaggcga ttccaagcag accggtcgcc aacagcgtga
     7441 tcgtgaggcg cgagccgccg atgatccgcg gctccgctgc gctcgcttcc atcggcgccg
     7501 gaccagccgc cgagccgagc gcaagcacgc cggcggcgca cacggcgttc acgctggcga
     7561 gacaaaccaa tgtgccggaa agtcctaatg cgggcagcag caaaaacgtg gaagtcaatg
     7621 tgccggcgac ggcgccgaag gtgttcgcac catatacccc ggcgctgacg cggccatcgc
     7681 cgcacgcctc gcgcacgagc cgttcaagcg cggtcaacgt tccgcccatt gctgccgtcg
     7741 ccggcaacag caccaaggtc ggcaacgcga agctggccgc ccacagcagg gccggcgcag
     7801 gctcggtgcc gagcaggggc gacaaggcgc ggccggctgc gggcagcagc cagatgcaga
     7861 tcaggcccca aaggccgatg accgcctcca atgcgatgta cgccgttcgc ggtgatctgg
     7921 tgcgccggat cacccggtcg agggcaaacg ccccaagcgc cagcccggcg aagaagccgg
     7981 caacggcgcc gagcaccgcc atcatttcgc tgccgagcgc gaggctcaac tggcgcgtcc
     8041 acacgatctc
//
LOCUS       DATNOI010000224         2266 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_947752,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000224 DATNOI010000000
VERSION     DATNOI010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947752"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VN968_08555"
     CDS             <1..378
                     /locus_tag="VN968_08555"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HXL29325.1"
                     /translation="EGEIDGAFVSLVQLQAGALVIAADAFLTSRREQLVALASRHTVP
                     AMYELREFAAVGGLISYAPSLTAAYRQVGIYASKILKGAKPADLPVMQAVKIELVINL
                     KTAKVLGLTFPITLLGRADEVIE"
     gene            375..1364
                     /locus_tag="VN968_08560"
     CDS             375..1364
                     /locus_tag="VN968_08560"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL29326.1"
                     /translation="MKRREFITLLGGTAAWPLAARAQQGERMRRIGVLMASAADDSEN
                     QARMAAFLQGLAQLGWTDGRNVLIDTRWATTNADDLRRHTAELAALAPDVLVAATGTA
                     TAAPLLQATRVVPIVFVIVIDPVGAGFVASLARPGGNATGFTAFEYGVSGKWLELLKE
                     VAPGVTRVAVLRDPAVASGIGQFAAVQAVAPSLGVELSPVDTRDAPEIERAVTVFARS
                     GNSGLIVTPSPVASRHRELIIALAARHKLPAVYGFRFYVTTGGLISYGPDPIDQYRRA
                     AGYVDRILKGEKPADLPVQAPIKYETVINLKTAKALGLEIPSSVLARADEVIE"
     gene            1361..>2266
                     /locus_tag="VN968_08565"
     CDS             1361..>2266
                     /locus_tag="VN968_08565"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL29327.1"
                     /translation="MKRREFIALLAGAAAAWPLGARAQQPVMALVGLLSSAQLDDRQI
                     GAIRQGLKDAGYIEGRNVAIKYRSADARFDRLPALAADLVADPVAAIIALAPPAAVAA
                     KAATATIPIIFATGADPVGLGLVSSFNRPGGNATGVTFVVNTLGAKRLELLRELVPSA
                     TVIGLLVNPGNPTSDSQTRDVQAAARAIGVEPLILNVGSERNIDAAFASAVQQRVNAV
                     IVGADSFFVSRSDQLVGLTARHAMPAVYFLREFADIGGLVSYGPSQTDAYRLAGGYAA
                     RILKGEKPADLPVVQSVKFELVVNLK"
BASE COUNT          374 a          740 c          736 g          416 t
ORIGIN      
        1 gaaggcgaga tcgacggcgc tttcgtctcg ctcgtccagt tgcaagccgg cgcgctcgtc
       61 atcgccgccg acgcgttcct caccagccgg cgcgagcagc tagtggcatt ggcatcacgc
      121 cataccgttc cggcgatgta cgagttgcgt gagttcgccg cggtcggcgg cctgatcagt
      181 tatgcgccga gcctcaccgc tgcctatcgc caggtcggca tttacgccag caagatcctc
      241 aagggcgcca agcccgccga cctgccggtc atgcaggcgg taaaaatcga gctggtgatc
      301 aatctcaaga ctgccaaagt gctcggtctc accttcccga tcacgctgct cggccgcgcc
      361 gacgaggtga tcgaatgaag cgccgcgagt tcatcacgct tctcggcggt acggccgcgt
      421 ggccgctcgc ggcgcgcgcg cagcagggcg agcggatgcg gcgcatcggc gtgctgatgg
      481 cctcggcggc ggatgattcg gaaaatcagg cccgcatggc ggcgttcctg cagggcttgg
      541 cgcaattggg ctggaccgac ggccgcaacg tgctcatcga cacccgctgg gccacgacca
      601 atgccgacga ccttcgcaga cacacggcgg aattggccgc gctcgcgccg gacgtcctcg
      661 tcgcggcgac tggcaccgca accgcggcgc cgttgctgca ggcgacccgc gtcgtgccga
      721 tcgtgttcgt gattgtcatc gacccagtcg gcgccggttt cgtcgcgagc ctggcgcgac
      781 cgggaggcaa cgcgaccgga ttcacggctt tcgaatacgg cgtgagcgga aaatggttgg
      841 agctgctcaa ggaggtggcg ccgggcgtga cgcgagtggc ggtcctccgc gatccggccg
      901 tcgcctccgg gatcgggcag ttcgccgccg tccaggcggt ggcgccgtcc ttgggggtgg
      961 agttgagccc ggtcgacacg cgcgatgcgc ccgaaatcga gcgcgccgtc acggtattcg
     1021 cgcgctccgg caatagcggc ctgatcgtga cgccgagccc agtggcgagc cgtcatcgcg
     1081 agctgatcat cgcgctggcg gctcggcaca agctgcccgc ggtctacggc ttccgtttct
     1141 acgtcactac gggcggcctg atttcttatg ggcccgatcc gatcgatcag taccggcgcg
     1201 cggccggcta cgtcgatcgc atcctcaagg gcgagaagcc ggccgacctg ccggtgcagg
     1261 cgccgatcaa gtacgaaacg gtgatcaatc tcaagaccgc caaggctctt ggcctcgaaa
     1321 ttccatcatc ggtgctcgcc cgtgctgacg aggtgatcga gtgaaacgac gcgagttcat
     1381 cgctctgctc gccggcgcgg cagccgcttg gccacttggg gcgcgcgcgc agcagccggt
     1441 catggcgttg gtcggcttgc tcagcagcgc ccagctagat gatcgacaga tcggcgctat
     1501 tcggcagggc ctcaaggacg ctggctatat cgaaggccgc aatgtcgcca tcaaatatcg
     1561 ctcggccgat gctcgcttcg accgattgcc ggcattggcc gccgatctgg tggccgaccc
     1621 cgtggccgcg atcattgcgc tcgccccgcc ggctgcagtg gccgccaaag ctgcgaccgc
     1681 aaccatcccg atcattttcg cgaccggcgc cgatccggtc ggtctcggtc tcgtttccag
     1741 tttcaaccgt cctggcggca atgccacggg cgtgactttt gttgtcaaca cactcggggc
     1801 aaaacggttg gaattgctgc gcgagctggt cccgagcgcc actgttatcg gcctcctcgt
     1861 caatcccgga aatcccacgt ctgactccca gactagagac gtgcaggctg cagcgcgcgc
     1921 aatcggcgtt gagcccctga tcctgaatgt cggcagcgaa cgcaatatcg acgcggcttt
     1981 cgcgagcgcc gtccaacagc gtgtcaacgc cgtcatcgtc ggcgctgatt cattctttgt
     2041 gagtcggagt gaccaactag tcgggctgac agcgcgtcat gcgatgcccg cagtctattt
     2101 tttgcgcgag tttgccgaca ttggtggact ggtaagctat ggacccagcc aaacggatgc
     2161 ctatcgcctc gccggtggct acgctgcgcg gattctgaag ggcgagaagc ccgccgactt
     2221 gccggtggtg cagtcggtca agttcgagct ggtcgtcaac ctaaag
//
LOCUS       DATNOI010000225         8660 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2380894,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000225 DATNOI010000000
VERSION     DATNOI010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8660
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2380894"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /locus_tag="VN968_08570"
     CDS             complement(<1..1609)
                     /locus_tag="VN968_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL29328.1"
                     /translation="MHASRLRGKSAAGVIAALVIALSAATPAAAQKRGGTLRLYHNDN
                     PPSTSLLEESTIASVTPFSAVFNNLVVFDPAKVHESIDTVVPDLAESWSWNATNTRLT
                     FKLRQGVKWHDGKPFTAKDVQCTWRMLIGKSEVSEFHRNPRKVWYGKLQDVTINGDYE
                     ATFELSEPQPSLPVLLASAFSVIYPCHIPQATMRTRPVGTGPFKLVEFNRGASIRLVR
                     NPDYWKKDRPYLDEITFRMIDSRATRMLAFATGEFDITFPADISVSLMKDVKARAPNA
                     ICEMITTGTQSNLMVNRVNPPFDNPEIRKAMSLAIDRKAFNTILMDGLALMGGAMLPK
                     PAGEWGMPPEMVSSLTGYGPDTDENIAQARAIMQKLGYSDAKPLGIKIQTRNLPNYRE
                     AAVIVTDQLKKIYIAGELDILETPQWYARLARKDYTIGLNVTGVSVDDPDGNIVENYS
                     CKSERNYTQYCNAEVDQLLAAQSRELDKEKRRKIVWDIERLLVDDAARPNIISNVAAN
                     CWQPYVRNYIPHDNSQYNSLRFEDVWLD"
     gene            1827..2924
                     /locus_tag="VN968_08575"
     CDS             1827..2924
                     /locus_tag="VN968_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL29329.1"
                     /translation="MTTRRDFLKAGTAAVTTGIVFCSCGLKQSAHAEQPTRQKLPVMV
                     GGKRVKTIDVHAHCHFREAGALLGADAAVQLPPVNGAAEAFIEIDKRLAAMDAQAIDL
                     EVLSINPYWYNRERDLAAAIVKIQNEKLAELCASKPDRFAAFASLTLQAPDLAVQELE
                     TAVKKQGLKGAAIGGVVNGVEFSDPKFHPVWAKAEQLGVPLFIHPQGVPELAKRLSGN
                     GWLGNTIGNPLETTIALSHLIFEGTLDRFPGLKVIAAHGGGFLPSYADRSDHACLVGP
                     KGCNADVKLGKAPTEYLKQIYFDSLIFSPEAIRHLVAQVGAGQIVLGSDYPYPWQLQP
                     VDHIFASASLSDDDKADILGRTAAKLFELTT"
     gene            complement(3012..3302)
                     /locus_tag="VN968_08580"
     CDS             complement(3012..3302)
                     /locus_tag="VN968_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29330.1"
                     /translation="MTSFRPTKIQQFAIEAKLNLMFGANVYDRIFLGFEVLEVVDGEL
                     RGWAPTEHGAAVIDVHYASKLVWIAETVLKRPIRRVSVLVRGLKHNTSEQPL"
     gene            complement(3635..5341)
                     /locus_tag="VN968_08585"
     CDS             complement(3635..5341)
                     /locus_tag="VN968_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXL29331.1"
                     /translation="MSGPARGRRRRAITLRALWPWLGAILALTAFNTTFAQGQTLTPD
                     LLRPVRDGFLSPQDSPLRRTADGDLADEWRPRARDTPAPSRIGNIPTYGLAAASGASA
                     SGFDSLNRTRKKPKYYPGQARPKPPPGPGSRAPVISTGPARLSIPPSETANMPPISPA
                     MAGLVAGQPPRKRLRIDDDPFGAVGDYAGGFLVKSAVELSGGYDSNPGRTAVARGSPF
                     VVVAPEFLAVSDWERHAVVADLRGSFTGYGNNFPPPGDGTISSVPTKLDRPDFTGHID
                     GRLDVSHDTRLTGEVRLRVATDNPGSPNIQAGLAKYPVYTTLGSTVGVDQNLNRLQLS
                     AGGTLDRTVYTDSKLTDGTSTSNDDRNFNQFGGIGRVSYDLLPGVKPFAEAEGDSRVH
                     DVRLDRSGFARDSSGGYVKAGSSFEFSRLLTGELSLGYAARDYVDPRLDRLQGLLTSA
                     SLTWTATPLTTARFYSTTSIDETTVPGVSGVLTHIYTVEADHDFRRWLTAIGKFTWGS
                     LDYQGDGRRDKIYTVSGDLIYKMSRSLWVKGTLRRDWLDSNTPGNSTASTVAMLGVRV
                     QN"
     gene            5607..6629
                     /locus_tag="VN968_08590"
     CDS             5607..6629
                     /locus_tag="VN968_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HXL29332.1"
                     /translation="MANPKPLMADSSGTDDANAAVASALRTLDAGGSGIAAIAAALAG
                     PLGPAFTAAVDLIREAKGRVIVTGLGKSGHVARKIAATLASTGTPAFFVHAAEASHGD
                     LGMITADDVIIALSWSGEQPEMKNLVNYSKRFAIPMIAVTSNAASPLGQAARIALELP
                     KAREACPHNLAPTTSSLMQAATGDALAIALLEGRGFTALEFANFHPGGKLGAMLKFVR
                     DLMHTGDAIPVKPLGTKMSDALVEMSSKGFGCVCIVDGLGEIAGIITDGDLRRHMRPD
                     LMTALVDEVMTKNPRTIAPGMLASDVMRESIDSSKPAVMVLIVAEDNKPVGIVHVHDV
                     LRAGVA"
     gene            complement(6668..7213)
                     /locus_tag="VN968_08595"
     CDS             complement(6668..7213)
                     /locus_tag="VN968_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXL29333.1"
                     /translation="MSHIAPLEPPYAPEIQQQFDRIMRGAPPLMLFRVVAGNARAWEK
                     FRAGSLLDRGPLSPREREIVIDRTCALTGCEYEWGVHVSAFAEAAHLTDAQVRATVLE
                     GADAPCWSAAEQALIAAVDALHLRACLSDNEFKALSAHYDDAKILEIILLCGFYRTVS
                     YLANGLDLPLEANAARFPVAN"
     gene            7286..7627
                     /locus_tag="VN968_08600"
     CDS             7286..7627
                     /locus_tag="VN968_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL29334.1"
                     /translation="MPKQISAVKKPAVRGSRSGRPIMALLDLLGRRWTLRIIWELREQ
                     PLTSRALRAACDEASPTVLQARLSDLRQAGLVELMPASGYRLTAMGRELHETFLPLHR
                     FAERWSKLKAN"
     gene            complement(7631..8077)
                     /locus_tag="VN968_08605"
     CDS             complement(7631..8077)
                     /locus_tag="VN968_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HXL29335.1"
                     /translation="MTEMLYTLGTWNWPIFGLILMALELLTPGVFLFWIGLAALLVGL
                     ISFAIDPPWQLQFLMFAAFATAAVPLWRRLARNNTAASTSNPFLNKRAEALVGRVFTL
                     EKPIIDGWGTVRIDDTVWRVAGPDTPAGSRVRIVQADGARLTVVSI"
     gene            complement(8088..>8660)
                     /locus_tag="VN968_08610"
     CDS             complement(8088..>8660)
                     /locus_tag="VN968_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXL29336.1"
                     /translation="VSPWGLKVNRIEIKDIVPPADLVEAMGRQMKAERVKRADILQAE
                     GQRQSEILRAEGAKQGQILQAEGRREAAFRDAEARERSAEAEAKATQMVSEAIAKGDV
                     AALNYFIANNYIKAFGQFADAPNQKVIMLPIEAVSLLGSLAGIGEIAKATFGESAASA
                     QAAAHRPSVPQAGPNPQLAGPNPPPATPQP"
BASE COUNT         1567 a         2770 c         2762 g         1561 t
ORIGIN      
        1 tgtccagcca gacgtcctcg aaccgcaggc tgttgtattg gctgttgtcg tgcgggatgt
       61 aattcctgac atagggttgc cagcagttcg cggccacatt ggagatgatg ttcgggcgcg
      121 ccgcgtcgtc gaccagaaga cgctcgatat cccagacgat tttccgacgc ttctccttgt
      181 ccagttcgcg ggactgcgcg gccagcagtt gatccacctc ggcattgcag tactgggtat
      241 aattgcgctc cgacttgcag gaatagttct cgacgatatt gccgtcggga tcgtcgacgc
      301 tgacgccggt gacgttcaat ccgatcgtgt aatccttccg cgcgagccgc gcataccatt
      361 gcggcgtttc cagaatatcc agctcgccgg cgatgtagat cttcttgagc tgatcggtga
      421 cgatcaccgc ggcttcgcga tagttcggaa gatttcgcgt ctgaatcttg attcccagcg
      481 gcttggcatc gctgtagcca agcttctgca tgatcgcgcg ggcttgcgcg atattttcgt
      541 cggtgtcggg accatacccc gtcagcgacg acaccatttc cggcggcatg ccccattcgc
      601 ccgccggctt cggcagcatg gccccgccca tcagcgccaa tccgtccatc aaaatcgtgt
      661 tgaaggcctt gcgatcgatc gccagcgaca tggctttgcg gatctccgga ttgtcgaacg
      721 gcggattgac gcggttgacc atcaggttcg attgcgtgcc agtggtgatc atctcgcaga
      781 tcgcgttcgg cgcacgcgcc ttgacatcct tcatcaggga aacgctgata tccgccggaa
      841 aggtgatatc gaattcgccg gtcgcgaatg ccagcatccg cgtcgcgcgg ctgtcgatca
      901 tccgaaacgt gatctcgtcc agataggggc ggtccttttt ccagtagtcg ggattgcgca
      961 ccaggcggat cgaagcgccg cggttgaact cgaccagttt gaacggcccg gtaccgaccg
     1021 ggcgggtgcg catggtcgct tgcggaatgt ggcacggata gatgacggaa aaagcgctcg
     1081 ccagcagcac cggcaggcta ggctgcggct cgctcagctc aaaggtggcc tcgtaatcgc
     1141 cattgatcgt gacgtcctgc agcttgccgt accagacctt gcgcgggttg cggtgaaatt
     1201 cggaaacctc gctcttgccg atcagcatcc gccaggtgca ttgcacgtcc ttcgccgtga
     1261 agggcttgcc gtcgtgccat ttcacgccct gccgcagctt gaaggtcagc ctggtattgg
     1321 tcgcgttcca cgaccagctt tccgccagat ccgggaccac ggtatcgatg ctctcatgga
     1381 ccttggcggg atcgaaaacc acgagattgt tgaagacggc cgaaaacggc gtcaccgaag
     1441 cgatggtcga ttcttcgagc aatgaagtgc tgggcggatt gtcgttgtga tagagccgca
     1501 gagtgccgcc ccttttttgt gcggccgccg gcgttgcggc cgatagagcg atgaccagag
     1561 ccgcgatgac gccagctgct gacttgcctc gcaaccggct ggcgtgcatt tgagcctccc
     1621 aaaaggtttt tgtttttcta tagggccgcc gccgatcggg cgcaacgtgg cgcggcgcga
     1681 acagaccgtc ttgccgccgg cccatggcgt gccttacgct agcctccagt ctggccgtcc
     1741 gcccgcgcac tgcggtggat cgagaccggg tacggcgtgg tggtggcaga ccgcgccagc
     1801 caacaacagc catgggagga attgtcgtga ccacacgccg tgattttctg aaagccggca
     1861 ccgccgccgt caccaccggc attgtcttct gcagctgcgg cctaaagcag agcgcgcacg
     1921 ccgagcagcc gacgcgccaa aagctacccg tcatggtcgg cggcaagcgg gtcaaaacca
     1981 tcgacgtgca cgcccattgc cacttccgcg aagccggcgc gctgctcggc gccgatgccg
     2041 cggtccagct tccgccggtc aacggcgcgg ccgaggcgtt catcgagatc gacaagcggc
     2101 tcgccgcgat ggacgcgcag gccatcgacc tcgaagtact gtcgatcaat ccctactggt
     2161 acaaccgcga gcgcgatctg gccgcagcga tcgtgaagat ccagaacgaa aagctggcgg
     2221 aactctgcgc ctcaaagccc gaccggttcg cagccttcgc ctcgctcacc ttgcaggcgc
     2281 ccgatctcgc ggtgcaggaa ctcgagaccg ccgtgaagaa acagggattg aagggcgctg
     2341 cgatcggcgg cgtggtcaac ggcgtggaat tctccgaccc aaaattccat ccggtgtggg
     2401 cgaaggcgga acagctcggc gtgcccttgt tcatccatcc gcagggcgtt cctgaacttg
     2461 ccaagcgatt gtccggcaac ggctggctcg gcaacaccat cggcaatccg ctggagacga
     2521 cgatcgcgct ctcgcacctc atcttcgagg gcacgctcga tcgctttccc gggctgaaag
     2581 tgatcgccgc gcatggcggc ggcttcctgc cctcctacgc cgatcgttcg gaccatgcct
     2641 gcctggttgg ccccaagggc tgcaatgccg atgtgaaact cggcaaggcg ccgacggaat
     2701 atctcaagca gatctatttc gattcgctga tcttctcgcc cgaggcgatc cgccatctgg
     2761 tggcccaggt cggcgcaggg caaatcgttt tgggcagcga ctatccctat ccctggcagc
     2821 tccagccggt cgaccatatt tttgcctccg cctcgctcag cgacgacgac aaggccgaca
     2881 tcctcggccg taccgcggca aaactgttcg agctcacgac gtagcgcggc tggcgggaag
     2941 ccgccgtgcc tcgtgcgccc gcgccggcgc cgctgcgctc tcccttgaat acctctgtgc
     3001 gtaccgggtg ctcacagagg ctgctcgctg gtattatgct tcaagccgcg cacgagaacg
     3061 ctgacccttc gaatcggccg ttttaatacg gtctcggcga tccagacaag cttggaggcg
     3121 tagtgaacgt cgatgaccgc cgcaccatgc tcggtgggag cccagccgcg cagctcgccg
     3181 tcgacaacct caaggacctc gaacccgaga aagatcctgt catagacgtt ggctccgaac
     3241 atcaggttca gcttcgcttc gatggcgaat tgctgaattt tggttggacg aaatgaggtc
     3301 atgcgggagt cccttgatga ccataagtac ggtcggcgcc taaaaaggtt cactgtgggc
     3361 gtatgcggca atcgcgcgct atcagagaag cgcgtcgcgg gaaccgccgc ggcccccagg
     3421 acttccgcag gccaacggtg gacctcggtc cgcattttgt aggcgaggcg ttggagggat
     3481 atgacgacaa ctttggggca gcaccgtgcg ccacagcacg caacgcgtca tgcgcgcggg
     3541 catgacccgc gcatcgatcg atcctcacaa gaaacatttt ttcgaaggga tatggatggc
     3601 cggatcaggt ccggccttga cgcaggcaga gtccctagtt ctgcaccctt accccgagca
     3661 tcgccacggt cgatgccgtg ctgttgccgg gagtgttgct gtccagccag tcgcggcgca
     3721 gcgtgccttt gacccagagg ctgcggctca tcttgtaaat gagatcgccc gagaccgtgt
     3781 agatcttgtc gcggcggccg tcgccctgat agtcgagcga accccaggtg aatttgccaa
     3841 tggccgtgag ccagcggcgg aaatcgtgat cggcctccac ggtatagatg tgcgtcagca
     3901 cgccggagac accgggcacc gtggtctcgt cgatcgaggt ggtggaatag aaccttgccg
     3961 tggtcagcgg cgtcgcggtc caggtcagcg aggctgacgt gagcaggccc tgcaggcggt
     4021 cgagccgcgg atcgacgtag tcacgcgcgg cgtagccgag cgagagttca ccggtcagca
     4081 gccgcgaaaa ttcgaaagag ctgccggcct tgacgtagcc gcccgaggaa tcgcgggcaa
     4141 agcccgagcg gtcgagcctg acgtcgtgca cgcggctgtc gccctcggcc tcggcgaacg
     4201 gcttgacgcc gggcaggaga tcgtagctga cgcggccgat gccgccgaat tgattgaagt
     4261 tgcggtcgtc attgctggtc gaggtgccgt cggtgagttt tgaatccgtg tagacggtgc
     4321 ggtcgagggt gccgccggcg gaaagctgca gccgattaag gttctggtcg acgccgacgg
     4381 tgctgccgag cgtcgtatag accggatatt tggcaagccc ggcctggatg ttcgggctgc
     4441 cgggattgtc ggtggcaacg cgcaaccgca cctcgcccgt caggcgggtg tcgtggctga
     4501 cgtcgagacg gccgtcgata tggccggtga aatccggccg gtcgagcttg gtcggcaccg
     4561 atgagatggt gccgtcgccc ggcggcggaa aattgttgcc gtagccggtg aacgatcccc
     4621 gcaagtcggc gaccacagcg tgacgctccc agtcggagac cgcgagaaat tccggcgcca
     4681 ccacgacaaa cggcgatccc ctggcgacgg ccgtgcggcc gggattgctg tcatagccgc
     4741 cactgagctc gaccgcggat tttaccagaa agccgccggc gtagtcgccg accgcgccaa
     4801 acgggtcgtc gtcgatcctc aagcgcttgc gcggcggctg gccggcgact aagcctgcca
     4861 tcgcaggcga aatcggcggc atgttggcgg tctcggacgg cgggatcgac agccgggccg
     4921 ggccggttga aatcaccggc gcacggctgc cgggacccgg cggcggtttc ggcctggctt
     4981 ggcccggata gtatttcggt ttcttgcggg tgcggttgag cgagtcaaag ccggaggcgg
     5041 aggcgccgct ggcggcggca aggccgtagg tcgggatatt gccgatgcgg gaaggcgcag
     5101 gcgtatccct ggcgcgcggc ctccattcgt cagccaaatc cccgtcggcg gttcggcgca
     5161 gcggcgaatc ctgcggcgac agaaaaccgt cgcgcacggg acgcagcagg tccggggtga
     5221 gggtctggcc ctgggcgaaa gtggtattga acgcggtcag cgcaaggatc gcaccgagcc
     5281 atggccataa cgcgcgcaaa gtgattgcgc gccggcggcg gccccttgct ggacccgaca
     5341 tcacgatgaa agatacccca gccaaatcat ctacttaggc gacgatttcc tgccccgcgc
     5401 ggacccgcct ccagggaaaa cgtcgttaac ggagttaaaa caagtatggt taatgggcgg
     5461 ttgagaacgg ggcgtccgcg tcgcatcgcc ggtccggcca tgctaaggag gtgccgggtc
     5521 gagaccgacg cccctcccct ggaacgcagg actcccctgg aacgcaagac tcccctggaa
     5581 cgcaagagtc ccttggaaca tatgacatgg ccaatccgaa accgctgatg gcagattcat
     5641 ccggcaccga tgacgcgaac gccgccgtgg cgtcggcgtt gcgcacgctc gatgccgggg
     5701 gcagcggcat cgccgcgatt gccgcagccc ttgccggtcc gctcgggccg gccttcaccg
     5761 ccgcggtcga tctcattcgg gaagccaagg gccgcgtgat cgtcacgggc ttgggcaaat
     5821 ccggccatgt ggcacgcaag atcgccgcca cgctggcctc caccggcacc ccggccttct
     5881 tcgtgcacgc cgcagaggcc agccatggcg atctcggcat gatcaccgcc gacgacgtca
     5941 tcatcgcgtt gtcgtggtcc ggcgagcagc cggaaatgaa aaacctcgtc aattattcca
     6001 agcgcttcgc tataccgatg attgccgtga cctcgaacgc ggcctctccg ctcggccagg
     6061 ccgcgcggat cgcgctcgaa ctgccgaagg cgcgcgaggc ctgtccgcac aatctggcgc
     6121 cgaccacatc gtcactgatg caggccgcga ccggcgatgc gctcgccatc gcgctgctcg
     6181 aaggccgcgg cttcaccgcg ctggaattcg ccaatttcca tcccggcggc aagctcggcg
     6241 cgatgctgaa attcgtccgc gacctcatgc ataccggcga cgccatcccg gtgaagccgc
     6301 tgggcaccaa aatgtcggac gcgctggtgg agatgtcgtc gaagggattc ggctgcgtct
     6361 gtatcgtcga cggtctgggc gagatcgccg gcatcatcac cgacggcgat ctgcgccgtc
     6421 acatgcggcc cgacctgatg accgccctgg tcgatgaggt gatgacgaaa aacccaagga
     6481 caatcgcccc cggcatgctc gccagcgacg tcatgcggga gagcatcgat tcgtcgaagc
     6541 cggcggtgat ggtgctgatc gtcgccgagg acaacaagcc ggtcggcatc gttcacgtcc
     6601 acgacgtgct gcgcgccggg gtggcgtaaa cggaccgtca tgccgaagat gcgacccgga
     6661 atgacggtta gtttgccacc ggaaaccgcg ccgcattcgc ctccagcggc agatcaagcc
     6721 cgttcgccag atacgacacg gtgcgataga agccgcacag caggatgatt tcaaggatct
     6781 tcgcatcgtc gtaatgcgcc gacagcgcct tgaattcatt gtcgctgagg caggcgcgga
     6841 gatgcagcgc atcgaccgcc gcgatcagcg cctgctcggc ggccgaccag cacggcgcgt
     6901 cggcgccctc cagcacggtg gcgcggacct gcgcatcggt gagatgcgca gcctcggcaa
     6961 aggcgctgac atgcacgccc cattcatact cgcacccggt caacgcgcag gtccggtcga
     7021 tgacgatttc gcgctcccgc ggcgagagcg ggccgcggtc cagcagactg ccggcgcgga
     7081 atttctccca ggcccgcgcg ttgccggcga ccacgcggaa cagcatcagc ggcggcgcac
     7141 cgcgcatgat gcggtcgaat tgctgctgaa tctccggcgc atagggcggc tcaagcggcg
     7201 cgatatgtga catcgccaac tcccgtgcta cattaaacgt agcatcatgc tacaatatat
     7261 gtagcaaact gcaagaagga cttcgatgcc gaaacagatt tcagccgtga aaaagcccgc
     7321 cgtgcgcggc tcccgctcgg gccgcccgat catggcgctg ctcgacctgc tgggccggcg
     7381 ctggaccttg cggatcatct gggagcttcg cgagcagccg ctgacgtcgc gcgcccttcg
     7441 cgccgcctgc gacgaggctt cccccacggt gctgcaggcc cggctgtcgg atctgcggca
     7501 ggcgggcctg gtcgaactca tgcccgcaag cggatatcgc cttacggcga tgggccgcga
     7561 attgcacgaa accttcctgc ctctgcaccg ctttgccgag cgctggagca aactcaaggc
     7621 caattgatct tcaaatcgac accaccgtca gcctggcgcc atcggcctgc acgatcctga
     7681 cgcggctgcc ggcgggggta tcggggccgg cgacgcgcca gaccgtatcg tcgattctta
     7741 cggtgcccca cccgtcgatg atcggctttt ccagcgtgaa cacccggccg accagcgcct
     7801 cggcgcgctt gttgagaaat ggattgctcg tgctcgcggc ggtattgtta cgcgcaagcc
     7861 gccgccacag cggcaccgcc gccgtggcga acgccgcaaa catcaggaac tgcaactgcc
     7921 agggcggatc gatcgcgaac gatatcaacc cgaccagtaa cgcagcgagc ccgatccaaa
     7981 acaggaagac gccgggtgtc agcagctcca gcgccatcag gatgaggccg aagatcggcc
     8041 agttccaggt gcccaacgtg taaagcatct cggtcatgat atgaccctca gggttgcggc
     8101 gtcgccggcg gcgggttcgg gcccgccagc tgcgggttcg ggcctgcttg cggcaccgac
     8161 ggacggtgcg cagcggcctg cgcggacgcc gcactttcgc cgaaggtggc cttggcgatc
     8221 tcgccgatac cggcgagcga gccgagcagg ctcaccgcct cgatcggcag catgatcacc
     8281 ttctggttcg gcgcatccgc aaactgcccg aacgccttga tgtaattgtt ggcaataaaa
     8341 tagttcagcg cggcgacatc gcccttggcg atggcctcgg agaccatctg cgtcgccttg
     8401 gcttccgctt cggcggaacg ttcgcgcgcc tcggcgtcgc ggaacgcggc ctcgcggcgg
     8461 ccttcggcct gcaggatctg gccctgcttg gcgccttccg cgcgaaggat ctcggattgc
     8521 cgctggccct cggcctgcag aatgtcggcg cgcttgacgc gctcggcctt catctggcgt
     8581 cccatcgctt cgacgagatc ggcgggcggc acgatgtcct tgatctcgat gcggttgact
     8641 ttcaatcccc acggcgacac
//
LOCUS       DATNOI010000226        11248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_222913,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000226 DATNOI010000000
VERSION     DATNOI010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11248
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222913"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..154
                     /locus_tag="VN968_08615"
     CDS             <1..154
                     /locus_tag="VN968_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL29337.1"
                     /translation="VARQRVQSSSEAIERVAQVTGFRDPERMRRAFIRAFGQPPQSLR
                     RAARAG"
     gene            complement(179..640)
                     /locus_tag="VN968_08620"
     CDS             complement(179..640)
                     /locus_tag="VN968_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29338.1"
                     /translation="MRNPFGVMDVPDPNDEEVMLFAGSTTLEGSADDENAKSWCTADN
                     GGQHDMIEGPWSSRWNGGADPTIAGDAANKWKQGRAEARTTGERVYLLFDWDSGARKG
                     LIDARREGPQRLVGKYINLSNPAITRPWIGLIVNNQRIDGRWTQGRLDFRR"
     gene            complement(939..2186)
                     /locus_tag="VN968_08625"
     CDS             complement(939..2186)
                     /locus_tag="VN968_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HXL29339.1"
                     /translation="METSASIEIAKHTLLSCGLILAIGTVAGLLARKIRIPDVAVFLI
                     TGIAIGPQALGLVHIGADSALNQIILLFGASYILFDGGASLRFDVLKQVWITIVVIAT
                     VGVIITAAITGIAAHYILGVPLIVALLLGATLASTDPATLVPIFRQVRIRDRVAQTVM
                     SESAFNDAMGAIVTFGVLAVATGSGEFSLASSMFDLLKQSAVGIVAGIVLGYVAAFLI
                     AHEKWAFLAEYAPVVTLVAVIGAYFAADGMEASGFMAVFVFGIVLGNKETFGFKMEAG
                     EAQKLDEYILTTAFIMRLFIFILLGAQVDFALMSKYWLGGVAVVTVLMLVARPATVFL
                     CALPDRRARWSVGEMLFMCWTRETGVIPAALAGLLLGMKAPGAQMIASVTFIAILMTI
                     LIQAPSTEWLGRRVGLLETKARS"
     gene            complement(2508..3455)
                     /locus_tag="VN968_08630"
     CDS             complement(2508..3455)
                     /locus_tag="VN968_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL29340.1"
                     /translation="MPVTLDPDAAAVFKAFQEAGRPPYETVSPAEAREFYSQARFVSN
                     PEPPELDQVQPLTIPSPAGPIPARIYTPRKLRKADGMAPCLVFFHGGGWVIGDLDSHD
                     VVCRKLADEGELIVMSVDYRLAPEHKFPAAVDDAIAATRWIAEHAKQHGIDASRLMVG
                     GDSAGGNLAAVVAIAARDGNGPDIAGQVLIYPATDFAMTHASHREPETSILLTHSVIR
                     WFRDHYLNGTADVHDWRASPARAGTLVGLPPAYVLTAGADPLRDEGDEYAHRLTEAGV
                     PVTYRHFPGQFHGFFTMGKLLPQANVAASEIGVWLKALN"
     gene            complement(3599..4378)
                     /locus_tag="VN968_08635"
     CDS             complement(3599..4378)
                     /locus_tag="VN968_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL29341.1"
                     /translation="MPDRIRLDGKVAVVTGAAGVIGTATMRLLAARGARIVAVDRKAA
                     DLQSAVKDLPASAQALAVPADVTSEDEVADYVRAAVDQFGTVDVFYNNAGIEGDVAPI
                     TKYSLETFRRVIDVNVVGVFLGMKHVLPVMLKQDRGSIINTASIAGLIGSPHIAVYSA
                     SKHAVIGLTRSAAWECTATGVRVNCVCPGMIDSRMLSAIIEGRNPGNAPTPNEKIVER
                     IPARRLGHASEVASIVAFLASDEASYVSGSAYTVDGGRTAA"
     gene            4610..5575
                     /locus_tag="VN968_08640"
     CDS             4610..5575
                     /locus_tag="VN968_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HXL29342.1"
                     /translation="MFRTIALISIAAFVAATTISLAPPARAQIGNIFSEPTPRPPGNI
                     PRGGNQPPPDDEEEVPELPQGRLLPTPNRLPPGQGVPAPGNIQSQPLAPPPGTTVVPQ
                     NAPPGVAVAPPQPNQGVAVAPPGAAPPPGLPPGQKQPKGVPQSPATLQPGDEVVSEPP
                     AQKIVNKKASFSGLDKITGRIINFDEDIGETIQFGALRVKTDACYTRPATEAANTDAF
                     VEVDEITLQGEVKRIFSGWMYAASPGLHGVEHPIYDIWLTDCKAPDATVVSAQPDAPR
                     VASPPPAQKRAQKQPPRQQPQPFQPPPQDLPPPQRQQSLFPPFGR"
     gene            complement(5602..6279)
                     /gene="aat"
                     /locus_tag="VN968_08645"
     CDS             complement(5602..6279)
                     /gene="aat"
                     /locus_tag="VN968_08645"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HXL29343.1"
                     /translation="MTSRDSASSEITPEVLLRAYACGIFPMAESADDPTLFWVEPEMR
                     GVIPLDGLRIPSRLARTVRSDAFTVTVNTAFKAVIAGCAAPQPGRDDTWINKRIRDLY
                     IGLHQLGHGHSVEVWQNDDLVGGLYGVSLGRAFFGESMFHRARDASKVALVHLVARLI
                     AGGFQLLDTQYVTEHLRSLGAIEISRRRYRGLLDKAVAGEADFAKLPVDRPIGGAKAL
                     EIIAERH"
     gene            complement(6314..7405)
                     /locus_tag="VN968_08650"
     CDS             complement(6314..7405)
                     /locus_tag="VN968_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL29344.1"
                     /translation="MNPTKPTLLYIDDDAALARLVERGLTRLGFTVVHAASGTQGLDR
                     IQQGGIDVVALDQYMPGLDGLETLDRILGIPNAPPVVFVTASQDSAIAVTALKAGAAD
                     YLVKDAQGDFIPLLQVAVNGALRQARIQKARDDAEAEVHASRDRYAALAAEREVLLRE
                     VNHRVGNSLQIIASLLHLQANSSTQDDVKAALTNAMGRVAAVAQVHRRLYTSHDLKSV
                     LLNQYLDALLEDLRRSAEGNKMSRLTLKAEPVEIDPDRAVAIGIIVNELVMNAVKYAY
                     PEGAGPIHVTLHAQGDDLLLAIADDGVGLNVKLDPRSTGMGQRIVSAMASKLEASVER
                     DPGHAGTRILLRFRRVNKPAAKPGCVAAS"
     gene            complement(7402..7842)
                     /locus_tag="VN968_08655"
     CDS             complement(7402..7842)
                     /locus_tag="VN968_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL29345.1"
                     /translation="MSKPVKIIMIEDDEGHARLIERNIRRSGVNNDILPFSNGTAAMN
                     YLFGGDGTGLDHRGEALLILLDLNLPDMTGIDILRRVKESKYLKCAPVVVLTTTDDSQ
                     EIKRCYELGCNVYITKPVNYESFANAIRQLGLFFSVIQVPQAAT"
     gene            complement(7839..9368)
                     /locus_tag="VN968_08660"
     CDS             complement(7839..9368)
                     /locus_tag="VN968_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HXL29346.1"
                     /translation="MMPDAPRWRATGQILLLAAGLVVLTVISAGSVYLVNKARDDARW
                     VVHTVEAENQISFTQLQLRRAESAQRGFLLTSQPNFQTDFEEAASQLAPALTRLGQLI
                     SDNPVERQLLGQMVALSNQRIEEFRQTIELARTQRLNDAAKMVREDIGREAMNRIYDL
                     AGGMRAEEDRLFVLRATSADRSQTLAASMTGIGSGFVVVLAGISIFLVRRSARARDDA
                     EALLRDNNLNLETTIDERTADLREANNEIQRFAYIVSHDLRSPLVNIMGFTSELEELR
                     GDIFRRIGTLGRAAASASPVPKIEGENEPVLDGEDRQLSQDFAEALGFIKSSIAKMDR
                     LISAILNLTREGRREFEPVRIETRELIEGIVATVAHQAAEAEAQIRIEPLPDIVSDRL
                     ALEQIFSNLIDNALKYLKPGVPGDISVRGRTKLGFAIFEIADNGRGIDPKDHQRIFDL
                     FRRAGTQDKPGQGIGLAHVRALVRRLGGTMSVASELHSGSTFTITLPIKWTASSRNRD
                     S"
     gene            complement(9502..10851)
                     /gene="accC"
                     /locus_tag="VN968_08665"
     CDS             complement(9502..10851)
                     /gene="accC"
                     /locus_tag="VN968_08665"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HXL29347.1"
                     /translation="MFDKILIANRGEIALRVLRACKELGISTVAVHSTADADAMHVRL
                     ADESVCIGPPPSKDSYLNVPALLAACEITGADAVHPGYGFLSENARFAEILADHNLHF
                     IGPKAEHIRLMGDKIEAKKTAKRLGIPVVPGSDGGVGPDDDAMAIAQTIGFPVLVKAA
                     SGGGGRGMKVAQTADDLLLALSTASNEAKSAFGDASVYLEKYLQKPRHIEIQVLGDGR
                     GGAIHLGERDCSLQRRHQKVWEEGPSPALDAASRAKIGATCAKAMQEMKYLGVGTVEF
                     LYEDGEFYFIEMNTRIQVEHPVTEMITDIDLVLEQIRIAAGGDLPVTQQEVIINGYAI
                     ECRVNAENPVTFRPSPGKILQYHPPGGLGVRIDSAVYQGYVIPPYYDSLVGKLIVHGK
                     TRAECLMRLRRALDEMVVEGIETTLPLFRALVREPAIIDGDYHIHWLEQYLAGQPAG"
     gene            complement(10964..>11248)
                     /locus_tag="VN968_08670"
     CDS             complement(10964..>11248)
                     /locus_tag="VN968_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HXL29348.1"
                     /translation="ASASASASVAVADLAKHPGVVPSPMVGTAYWASEPGAKPFIDVG
                     TKVSVGQTLLIIEAMKTMNQIPSPRAGTVTQILVEDGQPVEFGEPLVIIE"
BASE COUNT         2021 a         3549 c         3618 g         2060 t
ORIGIN      
        1 ggtggcgcga cagcgcgtgc aatcctccag cgaggcgatc gagcgggtcg cgcaggtaac
       61 cggctttcgc gatccggagc ggatgcgccg cgccttcatc cgcgccttcg gccagccgcc
      121 gcaatcgctg cgccgcgcgg cgcgggcggg gtgagccgaa ctgcgggaac tgaacgcttt
      181 atctgcggaa atccagacgc ccttgggtcc aacgtccgtc aattctttga ttgttcacga
      241 tcaagccgat ccagggacgt gtgatggctg gattgcttaa attaatgtat ttgccgacca
      301 gccgttgcgg gccctcgcgc ctcgcatcga tcagaccttt gcgtgcgccg gaatcccagt
      361 cgaacagaag gtagacgcgt tctcccgttg ttctcgcttc ggctcgaccc tgtttccatt
      421 tattcgcggc atcgcccgct atggtggggt ccgcgccgcc gttccagcgg ctcgaccaag
      481 ggccttcgat catgtcgtgt tggccgccgt tgtcagcggt gcaccaggac tttgcattct
      541 cgtcgtctgc ggacccctcc agcgtcgtac tgccggcgaa cagcatcacc tcctcgtcat
      601 tgggatcggg gacgtccatg acgccgaatg gatttctcat tggtttgctc caactgctga
      661 cagcgacgtc ttgcgccgac agacgcgggt tcgccgtttc cagagcgctc actcttgcaa
      721 atccggaggg tccgtcgcca cccgattccc gtccgcgccg ggttcgttca gccctcagca
      781 actgacatca accgcttgtt cagtgcaatg attcaaggcg cgctggcgtg gtgttcggca
      841 tcatggtggt cggggtccga gcaaacgaaa atccgcttgg gacccgaacc tatcgtgtac
      901 acacagccaa actctgcccg gcgcgccgtc aaagacagct acgaccgggc tttcgtttcc
      961 aacagcccga ccctgcggcc cagccattcg gtcgatgggg cctgaatcag gatcgtcatt
     1021 aaaatggcga tgaaggtgac ggaggcgatc atctgggcgc caggcgcctt catgccgagc
     1081 agcaggccgg ccagtgcagc gggaatgacg ccggtctcgc gggtccagca catgaacagc
     1141 atctccccaa cgctccagcg cgcgcgacga tccggcagcg cgcacaaaaa caccgtcgcc
     1201 gggcgcgcca ccagcatcag cacggtgacg acggcgacgc cgccgagcca gtatttgctc
     1261 atcaatgcaa aatcgacctg cgcgccgagc aggatgaaaa tgaaaaggcg catgatgaac
     1321 gccgtggtca ggatatattc gtcgagtttt tgggcctcgc cggcttccat cttgaagccg
     1381 aacgtctcct tgttgccgag cacgatgccg aacacgaaca ccgccatgaa gccgctggcc
     1441 tccatgccat cggcggcgaa ataggcgccg atgacggcga ccagcgtcac gaccggcgcg
     1501 tattccgcga ggaaagccca tttctcatgg gcgatgagga acgcagccac atagccgagc
     1561 acgataccgg cgacgatgcc gacggcggat tgcttgagca ggtcgaacat cgaggaggcc
     1621 aacgagaact cgccgctccc cgtcgccacc gccagcaccc cgaaggtgac gatcgcaccc
     1681 atggcgtcgt tgaatgccga ttcgctcatc acggtctgcg ccacccgatc gcgaatccgg
     1741 acctggcgga aaatcggcac cagcgttgcc ggatcggtcg aggcgagggt tgcgccgagc
     1801 agcagcgcca cgatcagggg gacgccaaga atgtaatgcg cggcgatgcc ggtgatagca
     1861 gcagtgatga tgacgccgac agtcgcgatc acgacaatgg tgatccagac ctgctttaag
     1921 acgtcaaagc gcagcgacgc gccgccatca aaaagaatgt agctggcgcc gaacagcagg
     1981 atgatctggt tcagcgccga atccgcgcct atatgcacga gccccagcgc ctgtgggccg
     2041 atcgcgatgc ctgtgatgag aaacaccgcg acatcgggaa tccggatctt ccgcgccagc
     2101 aggccggcga ccgtgccgat cgccaggatc agcccgcacg acagcagcgt gtgttttgcg
     2161 atttcgatcg aggccgacgt ttccaattcg ttatctcacc aggcgatgga aatagctggt
     2221 gcgcgccatt tccgcagatc atgatttgca aggccgcgac gctgccctac gcgccgtgaa
     2281 atggcaaggt cgccgccaat ctcgggacgt ttgccatatc aggaatcatc aaccagcccc
     2341 cttagagcac gagtctgtac ggacctttcg aaaccaacta agactgcacc ttcgttcatg
     2401 aggatttcgg gatgcctgtc gacgcaattc ctgctcggaa gcgtcttcgc aggcctggcc
     2461 atccgtttgg cgcttgccaa gcgccgctga cgcttttctt cgacgctcta gttcagcgcc
     2521 ttcagccaga cgccgatttc gctcgccgcg acattggcct gcggcagcag cttgcccatg
     2581 gtgaagaagc cgtggaattg gccgggaaaa tgccggtagg tcacaggcac gccggcttct
     2641 gtcaggcggt gtgcatattc gtcaccttcg tcgcgcaggg gatcggcgcc cgcggtcagc
     2701 acataggccg gtggcaatcc gacgagcgtc ccggcgcgcg cgggcgatgc ccgccaatca
     2761 tggacgtcgg cggtgccgtt cagatagtga tcgcgaaacc atcggatgac ggaatgcgtc
     2821 agcaggatgc tggtctcggg ctcgcggtgc gaagcgtgcg tcatcgcaaa gtctgttgcc
     2881 ggatagatca gcacctggcc tgcgatgtct ggcccattgc cgtcgcgcgc cgcgatcgcg
     2941 accacggccg cgaggttgcc gccggcgctg tcgccgccga ccatcagccg cgacgcgtcg
     3001 ataccgtgct gttttgcatg ttcggcgatc cacctggtcg cggcgatcgc gtcatcgacg
     3061 gcggcgggaa atttgtgctc gggcgcgagc cgataatcga cggacatcac gatgagctcg
     3121 ccttcatcgg ccaatttgcg gcacaccacg tcgtgggaat cgagatcgcc gatcacccag
     3181 ccgccgccgt ggaaaaacac caggcatggc gccatgccgt cggccttcct gagtttcctt
     3241 ggcgtgtaga tgcgcgccgg gatcggcccg gcaggcgagg ggatcgtcag cggctgcacc
     3301 tggtccagtt cgggcggttc ggggttgctg acgaagcgag cctgagaata aaactcgcgc
     3361 gcctcggcgg gcgaaaccgt ctcatagggc ggacggccgg cttcctgaaa ggccttgaag
     3421 acggcggcgg catcgggatc gagggtcacg ggcatggtga gaagacctgt tgatgctgag
     3481 ctgtaacgtt gtcattccgg ggcgcgcaaa gcgcgaaccc ggaatctcaa gattccccga
     3541 tgtgcaattg cacatctgag gtctggtccc ttcggaccat cccggaatga cggtgaagtc
     3601 aggccgcggt tcgtccgccg tcgacggtgt aggcggagcc ggagacatag cttgcttcgt
     3661 ccgacgcgag gaacgccacg atggaggcga cctcggacgc atgcccgagc cggcgcgccg
     3721 gaatccgttc gacgattttt tcgttggggg tgggcgcgtt gccgggatta cggccttcga
     3781 tgatcgcgct cagcatccgg ctgtcgatca tgccggggca gacgcaattc acgcgcaccc
     3841 cggtcgcggt gcattcccac gcggcactcc tggtgagccc gatcaccgca tgcttgctgg
     3901 cgctgtagac cgcgatgtgc ggcgatccga tcaagccggc aatcgatgcg gtgttgatga
     3961 tgctgcccct gtcctgcttc agcatcaccg gcagcacatg cttcatccca agaaacacac
     4021 cgaccacatt gacgtcgatc accctgcgaa acgtctccag cgaatatttc gtgatcggcg
     4081 cgacgtcgcc ctcgatgccg gcattgttgt agaagacgtc gacggtgccg aactggtcga
     4141 cggcggcgcg gacgtagtcg gcgacttcgt cctcgctggt gacgtcggcg ggcacggcga
     4201 gcgcctgcgc agaggccggc agatccttga ccgcgctttg cagatcggcc gccttgcggt
     4261 cgaccgcgac aatgcgcgcg ccgcgcgcgg ccaacagccg catcgtggcg gtgccgatga
     4321 cgccggcggc cccggtgacg acggcgacct tgccgtccag acgaattcga tcgggcatgg
     4381 gttaggtttc ctctgctgtt ccggttgttt ccggatttct gacggtccgg cgcagccgcg
     4441 cgaacgttga tcggggcgac tatttcatct gcggcggggg ctgaccagtg gccggaaccg
     4501 gcggtaaacg gccctttccc cgccgtattc ggcgtgttaa ccgggcccct gcgatcggcg
     4561 gataagtcta taaaatgtcg cgagcccgat tcgcctgtaa agccgcgaga tgtttcgaac
     4621 cattgccctg atcagtattg cggcatttgt tgccgccacc acgatctcgc ttgcgccgcc
     4681 cgcgcgcgcg cagatcggca atatattttc cgaaccgacg ccgcgcccgc ccggcaatat
     4741 tccccgtggc ggcaatcagc cgccacccga cgacgaagag gaagtgccgg aactgccaca
     4801 gggccgcctg ctgccgaccc ccaatcgtct gccgccgggg cagggtgtac cggcacctgg
     4861 caacatccag tcgcagcctc tggcgccgcc gccgggcacg accgtggttc cgcagaatgc
     4921 gccaccgggc gtcgcggtgg cgccgccgca acccaaccag ggcgtcgcgg tggcgccgcc
     4981 gggcgccgcg ccgccgccgg gattgccgcc gggacagaag cagcccaagg gcgtgccgca
     5041 atcgccggcc acattgcagc ccggcgacga ggtggtttcc gaaccgccgg cgcagaagat
     5101 cgtcaacaag aaggcgagct tttccgggct cgacaagatc accgggcgca tcatcaactt
     5161 cgatgaggat atcggcgaga ccatccagtt tggcgcgctg cgcgtcaaga ccgacgcctg
     5221 ctacacgcgt ccggcaaccg aggccgccaa taccgacgcc tttgtcgagg tcgacgagat
     5281 cacgctgcag ggcgaggtga agcggatttt ttcgggctgg atgtatgcgg cgagccccgg
     5341 cctgcatggt gtcgagcacc cgatctacga catctggctg accgactgca aagcccctga
     5401 cgccacggtc gtgagcgcgc agccggatgc gccacgagtg gcgtcgccgc ctcccgccca
     5461 gaagcgcgcg caaaagcagc cgccgcgtca gcagccgcag ccattccagc cgccgccgca
     5521 ggacctgccg ccgccgcagc gacagcagag tctgttcccg ccgtttggcc ggtaacgcgc
     5581 tttttggggc gtgcgctcgt gctagtggcg ttccgcgatg atctcgagcg ccttggcgcc
     5641 gccgatcggc cgatcgaccg gcaatttcgc aaaatccgct tcgcctgcga ccgccttgtc
     5701 gagcagcccg cggtagcgcc gccgcgagat ctcgatggcg cccaggctgc gcaaatgctc
     5761 ggtcacatat tgcgtgtcga gcaactggaa gccgccggcg atcagccgcg ccaccagatg
     5821 caccagcgcc accttcgagg cgtcgcgggc gcgatgaaac atgctctcgc cgaagaaggc
     5881 gcggccgagg ctgaccccgt acaatccgcc gacaaggtcg tcattctgcc agacctcgac
     5941 gctgtggcca tgtccgagct ggtgcagccc gatgtagaga tcgcggatgc gcttgttgat
     6001 ccaggtgtcg tcgcggcccg gctgcggcgc tgcgcagccc gcgatcaccg ccttgaacgc
     6061 ggtattgacg gtgacggtga aggcgtcgga gcgcacggtg cgcgccaggc gcgaggggat
     6121 gcgaaggccg tcgagcggga tcacgccgcg catttccggt tcgacccaga acagggtcgg
     6181 atcgtcggcg ctttcggcca tcgggaagat gccgcaggca taggcgcgca gcagcacttc
     6241 gggggtaatt tcggacgagg cggagtcgcg tgacgtcatg cacgacagga tagcagggtg
     6301 agggccgcgg cggctagctc gccgccacac aaccgggctt cgccgcgggc ttgttgacgc
     6361 ggcgaaaccg cagcaggatc cgggtgccgg catggccggg gtcgcgctcg acgctggctt
     6421 cgagcttgga ggccatggcg gagacgatgc gctgtcccat cccggtggaa cgcgggtcaa
     6481 gcttgacgtt gaggccgacg ccgtcgtcgg cgatcgccaa caggagatcg tcgccctgcg
     6541 catggagggt aacgtgaata ggtccggcgc cctcggggta ggcgtatttc acggcgttca
     6601 tcacgagctc gttgacgatg atgccgatcg ccaccgcgcg gtccggatcg atctccaccg
     6661 gctcggcctt caaggtcagc cgcgacatct tgttgccctc ggcggacctc ctgagatcct
     6721 cgagcagcgc gtcgagatac tggttcagga gcacgctctt gaggtcgtgg gaggtgtaaa
     6781 gccggcggtg aacctgcgcc accgcggcga cccgtcccat cgcgttggtc agcgccgcct
     6841 tgacgtcatc ctgggtgctc gaattggcct gcaggtgcaa tagcgaggcg atgatctgca
     6901 ggctgttgcc gacccggtgg ttgacctcgc gcagcagcac ctcgcgctcg gcggcaagcg
     6961 cggcatagcg gtcgcgcgag gcgtggacct cggcctcggc gtcgtcgcgg gccttctgga
     7021 tccgcgcctg ccgcagcgcg ccgttgacgg cgacctggag cagggggatg aaatcgccct
     7081 gggcgtcctt taccagatag tcggcggcgc ccgccttcag cgcggtgacg gcgatcgccg
     7141 agtcctgcga ggcggtgacg aacaccaccg gcggcgcgtt cgggattccc aatatccgat
     7201 ccagcgtttc aaggccgtcg aggccgggca tgtattgatc gagggctaca acgtcgatgc
     7261 cgccttgctg gatccgatcg agcccctgcg tgccgctcgc cgcatgaacg accgtgaagc
     7321 cgagccgggt cagaccgcgc tccaccagcc gggccagcgc cgcgtcgtcg tcgatgtaca
     7381 gcagcgtcgg cttcgtaggg ttcatgtggc ggcttgcgga acctggatga cggaaaagaa
     7441 cagcccgagc tggcgaatag cgttggcgaa gctctcgtag ttcaccggct tggtgatgta
     7501 gacgttgcaa ccgagttcat agcagcgctt gatctcctgg gagtcgtcgg tggtggtgag
     7561 caccacgacc ggcgcacatt tgagatattt gctctccttg acccgccgca gaatgtcgat
     7621 gccggtcatg tccggcagat tgagatcgag cagaatcagc agcgcctcgc ccctgtgatc
     7681 gaggccggtg ccatcgccgc cgaaaaggta attcatcgca gctgtaccat tgctgaacgg
     7741 caggatgtcg ttgttgacgc cggagcgacg gatattccgc tcgatcaggc gagcgtgccc
     7801 ctcatcgtcc tcgatcatga tgattttgac tggtttgctc atgaatctct gttccggcta
     7861 ctggctgtcc atttgatggg cagtgttatc gtgaatgtgc tgccgctgtg aagttccgat
     7921 gcaaccgaca tggttccacc aagtcgtcgc accaaagccc ggacatgggc aagtcctatg
     7981 ccctgtccgg gcttgtcctg ggtaccggcg cgacgaaaaa gatcgaaaat ccgctgatga
     8041 tccttgggat cgatgccgcg gccgttgtcg gcgatctcga aaatcgcaaa gcccagcttg
     8101 gtccggcccc ggacggaaat gtcgccggga actcccggct tgagatattt gagcgcgttg
     8161 tcgatcagat tggagaaaat ctgttccagc gcgaggcggt cgctgacgat gtccggcagc
     8221 ggctcgatgc gaatctgggc ttcggcctcg gcggcctggt gcgcgacggt ggccacgatg
     8281 ccttcgatca gttcgcgggt ctcgatccgc accggctcga attcgcgccg cccctcgcgg
     8341 gtgaggttga ggatggcgga tatcaggcgg tccatcttgg cgatcgacga tttgatgaat
     8401 cccagcgctt cggcaaaatc ctgcgaaagc tgcctgtctt cgccgtcaag gaccggctcg
     8461 ttctcgcctt cgatctttgg caccggcgaa gccgaagccg cggcgcggcc aagtgtccca
     8521 atccgcctga aaatgtcgcc gcgcagttcc tcgagttcgc tggtaaagcc cataatgttc
     8581 accagcggcg agcgcagatc gtggctgacg atataggcga agcgctggat ctcgttgttg
     8641 gcctcgcgaa gatcggcggt gcgctcgtcg atcgtggttt cgagattgag gttgttgtcg
     8701 cgcaacaggg cttcggcgtc gtcgcgggcg cgcgcggagc gccgtaccag aaagatcgag
     8761 atgccagcca gcacgacaac gaaacccgaa ccgatgccgg tcatcgacgc ggccaaggtc
     8821 tgactgcggt ctgcactcgt cgcgcgcagc acaaacagac gatcttcctc cgcccgcatc
     8881 ccgccggcca gatcgtagat gcggttcatc gcctcgcggc cgatatcctc gcgcaccatc
     8941 ttcgcggcgt cattcaagcg ctgggtccgg gcaagttcga tggtttgacg gaattcctcg
     9001 atccgctgat tgctgagggc aaccatctgg cccagcagct gcctttcaac gggattgtcg
     9061 cttatgagtt ggcccagccg cgtcagtgcc ggcgctagct gggatgccgc ttcctcgaaa
     9121 tcggtttgaa aattgggctg cgatgtcaga agaaaaccgc gctgcgcgct ttcggcccgg
     9181 cgcaattgca attgcgtgaa ggatatctga ttttcggctt caacggtgtg cacaacccag
     9241 cgcgcgtcat cccgcgcctt gttcaccaga tagaccgaac cggcgctgat aacagtcagc
     9301 accacaaggc ccgccgcaag cagcaggatt tggcccgtcg cacgccagcg cggggcgtca
     9361 ggcatcacga ccgtctcgcg tcataagcga acgaagttca agcagtcccc cgaacaggga
     9421 aagatgtttc ccagccaacc agaacgccgg ttcggtcaat tggttccagg gacctgcaac
     9481 cgatgtgggc taaacagcgg ctcaaccggc cggctggccg gccagatatt gctcaagcca
     9541 gtggatgtga tagtcgccgt cgataatggc gggttcgcgg accagggccc ggaacagcgg
     9601 cagcgtggtc tcgatgcctt ccaccaccat ttcgtccagc gcgcggcgca gccgcatcag
     9661 gcactcggcg cgggtcttgc catggacgat cagcttgccg accagcgagt cgtaataagg
     9721 cgggatgacg tagccctggt agaccgccga atcgatccgc acgccgaggc cgccgggcgg
     9781 atggtattgc aggatcttgc cgggcgaggg gcggaaggtc acggggtttt cggcgttgac
     9841 gcggcactcg atggcgtagc cgttgatgat gacttcctgc tgcgtcaccg gcagatcgcc
     9901 gccggccgcg atccggatct gctccagcac gaggtcgata tcggtgatca tctcggtgac
     9961 gggatgttcg acctggatgc gggtgttcat ctcgatgaaa tagaattcgc cgtcctcgta
    10021 gagaaattcc acggtgccga cgccgagata tttcatctcc tgcatcgcct tggcgcaggt
    10081 cgcgccgatt ttcgcgcgcg acgccgcatc gagcgccggc gaggggcctt cttcccagac
    10141 cttttgatgg cgccgctgca gcgagcagtc gcgctcgccg agatggatgg cgccgccgcg
    10201 gccatcgccg agcacctgga tttcgatgtg gcgcggcttc tgcagatatt tttccagata
    10261 gaccgaggca tcgccgaatg cggacttggc ttcattggag gcggtcgaca gtgccagcag
    10321 caggtcgtcg gcggtctgcg caaccttcat gccgcgtccg ccgccgcccg acgccgcctt
    10381 caccagcacc ggaaagccga ttgtctgcgc gatggccatc gcatcgtcgt caggaccgac
    10441 gccgccgtcg gagcccggca ccacgggaat gccgagccgc tttgcggtct tcttggcctc
    10501 gatcttgtcg cccatcaggc ggatgtgttc ggccttcggc ccgatgaaat gcagattatg
    10561 atcggcgagg atttcggcaa agcgggcgtt ttccgacagg aagccgtagc cgggatgcac
    10621 cgcgtcggcg ccggtgattt cgcaggccgc gagcagcgcg ggcacgttga gatagctgtc
    10681 ctttgacggc ggcggcccga tgcagacgct ttcgtcggcg agccgcacat gcatggcgtc
    10741 ggcgtcggcg gtggaatgca ccgcgaccgt ggaaatgccg agttccttgc acgcgcgcag
    10801 aacccgcaat gcgatttcgc cgcgattggc tatcaggatc ttgtcgaaca tgttgtccta
    10861 agcagcgaat agcgaaaggg ggcgaatggc aagtagcgag tagcgaatgg aaggaagcgc
    10921 cggcttcgtt tccctattcg ccattcgcca ctcgctattc gccctactca ataatcacca
    10981 gcggttcgcc gaattcgacc ggctggccgt cctcgacgag aatctgcgtc acggtgcccg
    11041 cgcgcggcga ggggatctgg ttcatcgtct tcatggcttc gatgatcagc agtgtctgtc
    11101 cgaccgacac cttggtgccg acgtcgatga acggcttggc gccgggctcg gaagcccagt
    11161 aggcggtgcc gaccatcggc gagggcacca cgcccgggtg cttggcgaga tcggcgacgg
    11221 cgaccgacgc tgaggccgac gcggaagc
//
LOCUS       DATNOI010000227         3951 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_397759,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000227 DATNOI010000000
VERSION     DATNOI010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397759"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VN968_08675"
     CDS             complement(<1..607)
                     /locus_tag="VN968_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXL29349.1"
                     /translation="MKSFKVTDFNAPLTEVDAPTPQPSGTQVLIKVKAAGVCHSDLHI
                     WEGGYDLGHGRKPLSLKDRGVSLPRTMGHETVGEVLAFGPDARGDTGGLKVGDVALVY
                     PWLGCGKCETCLAGEENMCLKPNSLGVYCDGGYADHMTVPHPRYLLNLKGLDPVTAAP
                     YACSGVTTYSALKKVEASFNTPIVIFGAGGLGLMALSLLKAM"
     gene            complement(680..1669)
                     /locus_tag="VN968_08680"
     CDS             complement(680..1669)
                     /locus_tag="VN968_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HXL29350.1"
                     /translation="MMHDIRQPALRDPLLTVENLVVEYTVGGKTIHAVSDVSLQVARG
                     ETLGLVGESGCGKSTLGRAVLQLRRAVSGRVLFDGQDLSAMQGDALRRMRRRVQLIFQ
                     DPIASLNPRRRIGDIVAEPLVIAGVKDPKNREQLVSEVLTAVGLDPGLVTGRLPHEFS
                     GGQCQRVCIARALVLNPEFIICDEPVSALDVSIRAQILNLLEDMKARFGLTLLFIAHD
                     LAVVKAVSDRVAVMYLGRLCEVGPSEQLFASPAHPYTALLIEAIPVPDPDVRPAESVA
                     VGEPPSPLAPPWGCRFRTRCPRADQRCSDEVPELRAVAAGQFAACHHPLIGAA"
     gene            complement(1666..2661)
                     /locus_tag="VN968_08685"
     CDS             complement(1666..2661)
                     /locus_tag="VN968_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL29351.1"
                     /translation="MTEPVLLSVEGVAVDLPTPRGNLRAVDQVDLKVGAGQTLGIVGE
                     SGCGKTMLSRAILQLLPKKAKLSGRVMFDGQDLVTLEPERLRKLRGRSLAVVFQDPMT
                     SLNPVLTIGSQLIETLQEHLELDGLLAKKRSVELLAAVGIPAPEQRLMQYPHQLSGGM
                     RQRVAIAIALSCEPKLLIADEPTTALDVTIQAQILDLLAREQRRRHMAMILITHDLGV
                     VAGRTDEVAVMYAGRVVERAPTPALFKNMRMPYTEALLAAIPKIDAAPHTPLPAISGR
                     PPDPTRPIQGCSFSPRCRYATGRCYQDKPLLIQAEAPDHQYACFHPIKAAAGVSA"
     gene            complement(2658..3527)
                     /locus_tag="VN968_08690"
     CDS             complement(2658..3527)
                     /locus_tag="VN968_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL29352.1"
                     /translation="MATLDLGIDENVAVRAINRTRRLGPLFWAAIAWMSLVFAAAALA
                     GLLPFSPTDMDMLERRAPPSALHWLGNDGLGRDELARLIYGARVSLTVGLCAPIIGIT
                     VGGALGMLAGYFRGRFETLVVGSMDVLLAFPPLILALAVTAYLGQSILNLTFILGVLG
                     IPAFMRVARAATLTLARREFVIAAQALGATHARILLRELLPNVILPLFAFFLLGVAVT
                     IVVEGALSFLGLGVPPPVSSWGSMIGEGRESLEMAPRLAFIPAVTMFLTVLSFNLVGD
                     TLRALTDPRQGAL"
     gene            complement(3514..>3951)
                     /locus_tag="VN968_08695"
     CDS             complement(3514..>3951)
                     /locus_tag="VN968_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL29353.1"
                     /translation="EDPIGNLRFFVLPALTLALAEWPGIMRVLRSDMIATLQEDYIAL
                     AKAKGLKASRILFVHALKPSSLTLVTITGINIGRLIGGALIVETIFALPGIGRLLVGA
                     IYTRDLIILQGVVLFVAAGFVLMNFIVDMFYAVLDPRIRHGHA"
BASE COUNT          781 a         1283 c         1269 g          618 t
ORIGIN      
        1 ccatggcttt gagcagcgac agcgccatca gacccagacc gccggcgcca aaaataacga
       61 tcggcgtgtt gaagctggcc tcgacctttt tcagggcgct gtaagttgtg acaccggagc
      121 acgcataggg cgccgcggtc acgggatcca gccccttcag attgagcaga tagcgcggat
      181 gcggcaccgt catgtggtcg gcatagccgc cgtcgcaata gaccccgagc gaattgggct
      241 tcaggcacat gttctcttcg ccggccaggc acgtctcgca tttgccgcag ccgagccagg
      301 gatagaccag cgcgacgtcg ccgaccttga gcccgccggt atcgcccctg gcgtccggcc
      361 cgaacgccag cacttcgccg acggtctcat gccccatcgt ccgcggcagc gacacgccgc
      421 ggtccttcag cgagagcggc tttcgtccat ggccgagatc gtagccgcct tcccagatgt
      481 gcaggtcgct gtggcagacc ccggccgcct tcaccttgat caggacctgg gtccccgatg
      541 gctgcggcgt cggcgcatcc acttccgtca aaggggcgtt gaaatcggtg accttgaaac
      601 tcttcatcgc ttcgctcccg tcaaaatttt cttgtattgt ccggactttg ccacgcattc
      661 ccaagcagtt gctagatgtt tacgccgccc cgatcaaggg atgatggcag gcggcaaact
      721 ggccagcggc gaccgcgcgc agctccggca cctcgtcgct gcagcgctga tcggcccggg
      781 ggcaacgggt ccggaaccgg cagccccagg gcggcgcgag cggcgatggc ggttcgccga
      841 ccgccacgct ttcggccgga cgcacgtccg gatcgggcac cgggatggcc tcgatcaaca
      901 gcgcggtata agggtgcgcc ggactcgcaa acagttgctc ggaggggccg acctcgcaca
      961 gccggccgag atacatcacc gccacccggt cgctgactgc ttttaccacc gcaagatcgt
     1021 gggcgatgaa gagcagcgtc agtccaaagc gcgccttcat atcctccagc agattgagga
     1081 tctgggcgcg aatggagacg tcgagcgccg aaaccggctc gtcgcagata atgaactccg
     1141 gattgaggac cagcgcgcgc gcaatgcaga cgcgctggca ctgtccgccg gaaaactcat
     1201 gcggcaggcg tccggtcacc agaccgggat cgaggccgac ggcggtgagt acttcggaga
     1261 cgagctgctc gcggtttttt gggtccttga cgcccgcgat caccagcggc tcggcgacga
     1321 tatcgccgat ccgccgccgc gggttcaagg acgcaatcgg gtcctggaag atcagttgca
     1381 cgcgccggcg catccgccgc aaggcatcgc cttgcatcgc cgagaggtcc tgaccgtcga
     1441 acagaacccg ccccgagaca gcccggcgca actgaagcac cgcgcggccg agcgtggact
     1501 tgccgcagcc ggactcgccg accagaccga gcgtctcgcc gcgcgcaacc tgcaggctga
     1561 cgtcggacac cgcatggatc gtcttgccgc ccaccgtata ttcaacgacg aggttttcca
     1621 ccgtgagcag cggatcgcgc aaagcgggct gcctgatatc gtgcatcatg cgctcacacc
     1681 ggctgcggct ttaatcggat ggaagcaggc atactgatgg tcgggcgctt ctgcttggat
     1741 aagcaagggt ttgtcctgat aacatcgtcc ggtggcataa cggcagcgcg gcgaaaacga
     1801 gcaaccctgg atcggacggg tcggatccgg cggccgcccc gaaatcgccg gcaagggcgt
     1861 atgcggggcg gcatcgattt tcgggatcgc cgccagcagc gcctcggtgt agggcatgcg
     1921 catgttcttg aacaaagcgg gcgtcggggc acgttccacc acccggccgg cgtacatgac
     1981 ggctacctcg tcggtgcggc cggcgacgac gccgaggtca tgggtgatca ggatcatcgc
     2041 catatgccgg cgccgctgtt cgcgcgccag cagatcgagg atctgggcct ggatggtcac
     2101 gtcaagcgcg gtggtcggct cgtcggcgat caacagtttc ggctcgcagg acagcgcgat
     2161 ggcgatggcc acgcgctgcc gcatgccccc cgaaagctga tgcgggtatt gcatcaatcg
     2221 ttgctccggc gcgggaatac cgaccgcggc cagcagttcc acactgcgct ttttcgcaag
     2281 caagccatca agttcgaggt gctcctgcag cgtttcgatc agctggctgc cgatcgtcaa
     2341 caccggattg agcgaggtca tcgggtcctg gaataccacc gcgagcgatc ggccgcgaag
     2401 tttgcgcaga cgttcgggtt caagggtcac gaggtcctgc ccgtcaaaca tcacgcggcc
     2461 ggaaagtttg gcctttttcg gcagcagctg caggatggcg cgcgacagca tggtcttgcc
     2521 gcatccggat tcgccgacaa tgccgagggt ctgaccggcg ccgactttca gatcgacctg
     2581 atccacggcg cgaagattgc cgcgcggcgt cggcagatcg accgccacgc cctcgacgga
     2641 aagcaacacc ggctcggtca cagcgcgccc tggcgcggat cggtcagcgc ccgcagcgtg
     2701 tcgccgacca ggttaaagga caatacggtc aggaacatcg tgacggcggg aatgaaggcg
     2761 agcctcggcg ccatttccag gctctcgcgt ccctcgccga tcatgctgcc ccaactcgac
     2821 accggaggcg gcaccccgag gcccagaaaa gacaatgcgc cctcgacgac gatggtgacg
     2881 gcaacgccaa gcaggaagaa ggcgaacagc ggcagaatga cgttgggcaa gagttcgcgc
     2941 agcaggatgc gcgcatgggt cgcgcccagc gcctgggcgg cgatcacgaa ttcgcgccgt
     3001 gccagggtaa gggttgccgc ccgcgcgacg cgcatgaagg cgggaatgcc gagcacgcca
     3061 agaatgaagg tgagattgag gatcgattgt ccgagatagg cggtcacggc cagcgccaat
     3121 atcagcggcg gaaaggcgag caggacgtcc atgctgccga ccaccaatgt ctcaaaccgg
     3181 ccgcgaaaat agcccgccag catgccaagc gcgccgccga ccgtgatccc gatgatcggc
     3241 gcgcaaaggc caaccgtgag cgaaacccgc gcgccataga tcagccgcgc cagttcgtcg
     3301 cgccccaggc cgtcattacc caaccagtgc agggccgacg gtggcgcccg ccgctccagc
     3361 atgtccatgt cggtcggact gaagggcaac aggccggcca gggccgcggc ggcaaaaacc
     3421 agactcatcc aggcaatcgc cgcccaaaac agcggcccga gccggcgcgt ccggttgatc
     3481 gcacggacgg cgacattctc atcgatgcca agatcaagcg tggccatgac ggatcctggg
     3541 atcgaggacg gcatagaaca tgtcgacaat aaagttcatc agcacgaagc cggcggcgac
     3601 gaacagcacc acaccctgca aaatgatcag gtcccgggtg tagatcgcgc cgaccagaag
     3661 ccggccgatc cccggcagtg cgaaaatcgt ctccacgatc agggcgccgc cgatcaggcg
     3721 gccgatattg atgccggtga tcgtcacgag ggtcagcgac gatggtttca gggcgtgcac
     3781 gaaaaggatg cgcgacgcct tcagtccctt ggccttggcc agcgcgatat agtcctcctg
     3841 cagggtcgcg atcatgtcgg aacggagcac gcgcatgatc cccggccatt cggcgagcgc
     3901 cagcgtcagc gcgggcagca cgaagaagcg caagtttccg atcggatctt c
//
LOCUS       DATNOI010000228         2335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1817857,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000228 DATNOI010000000
VERSION     DATNOI010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1817857"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1924)
                     /gene="yidC"
                     /locus_tag="VN968_08700"
     CDS             complement(110..1924)
                     /gene="yidC"
                     /locus_tag="VN968_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HXL29354.1"
                     /translation="MNDQKNMILAIVLSALVLLAWQYFVGLPQMEKQRQIAQQQQGQQ
                     PQGAPESRPPAPGGTPQLPGPPSAAPQAQLSREDALAKSKRIAIDTPRLSGSIALTGA
                     RIDDLSLIQYRETVDPRSPAIVLLSPSGSPHPYYAEFGWLKSTGDTRVPGSDTVWTQE
                     GSGGLGVGHPVTLVWDNGEGLIFRRTIAVDDKYLFTVKDEVTNKGEATVALNPFALIS
                     RHGTPPTLGYYILHEGLIGVIGDQGLKEITYKDVEKKNEEFSNVKNAWLGFTDKYWAA
                     ALLPQNSAQLLKAKFSAETLGTIKTYQTDYLLDAQTVPPGSTSTALGRLFAGAKEVAV
                     IDGYEKQLNLNRFEQLIDWGLFYFVTKPMFKLIDLIFHWVGNFGVAILIVTVLVKIAF
                     FPLANKSYASMAKMKAVQPQMQMIRERYGDDKVKQQQAMMELYKKEKINPVAGCLPMV
                     IQIPVFFALYKVLFITIEMRHAPFFGWIKDLSAPDPTSVFNLFGLLPFDPSHAPLVGQ
                     FLMIGAWPLVMGMTMWVQMKLNPAPPDPTQKMIFDWMPIIFTFMLANFSAGLVIYWAW
                     NNFLSVIQQSVIMRKNGAKIELFDNIKALFAKSGVKNQ"
     gene            complement(1937..>2335)
                     /gene="rnpA"
                     /locus_tag="VN968_08705"
     CDS             complement(1937..>2335)
                     /gene="rnpA"
                     /locus_tag="VN968_08705"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXL29355.1"
                     /translation="TERVIRPQPGPFVPMERLRQRTDYLAVATGARQPTPAFVLQGLR
                     RNDVGPPRFGFTVAKKVGKATERNRVRRRLKEMVRLAPAERMRAGYDYVLVGRRAALA
                     LPFDRLIAEFEGALERLHGGPRPYHRRAGG"
BASE COUNT          439 a          723 c          713 g          460 t
ORIGIN      
        1 cgaacgatcg cggaaatggc ggggcgctgc gatgctcctc accacgaggc cggaaaaggc
       61 cgcgatgctc ctcaccatga ggccgagaga gtctgggcac tgccagcaac tactggttct
      121 tgacccccga cttggcgaac agtgccttga tgttgtcgaa cagctcgatc ttggcgccgt
      181 tcttgcgcat gatcacgctc tgctggatga cggagaggaa attgttccat gcccagtaga
      241 tcaccaaccc ggcggaaaaa ttagccagca tgaaggtgaa gatgatcggc atccagtcga
      301 agatcatctt ctgggtcggg tcgggcggtg ccgggttgag cttcatctgc acccacatcg
      361 tcatccccat caccagcggc caggcgccga tcatgaggaa ctgcccgacg agcggcgcat
      421 gggaagggtc gaagggcaac aggccgaaca aattgaacac ggaagttggg tcgggcgcgg
      481 aaagatcctt gatccagccg aagaacggcg cgtgacgcat ctcgatggtg atgaacagca
      541 ccttgtagag cgcgaagaat accgggatct gaatcaccat cggcaggcac ccggcgaccg
      601 gattgatctt ctccttcttg tacagctcca tcatcgcctg ctgctgcttc accttgtcgt
      661 cgccgtagcg ctcgcgtatc atctgcatct gcggctgaac cgctttcatc ttcgccatcg
      721 acgcatagga tttgttggcg agcggaaaga aagcgatctt gacgagcacc gtgacgatta
      781 gaatcgccac gccgaaattg ccgacccagt gaaagatcag gtcgatgagc ttgaacatcg
      841 gcttggtcac gaaatagaac aatccccaat cgatcaactg ctcgaatcgg ttgagattga
      901 gctgcttctc gtatccatct atgaccgcca cttccttggc gccagcgaac agccgtccta
      961 acgccgtgct ggtgctgccc gggggcacgg tctgtgcgtc gagcagatag tcggtctggt
     1021 aggtcttgat cgtgccgagc gtttcggccg agaatttcgc cttcagcagt tgcgccgagt
     1081 tctgtggaag cagcgctgcc gcccagtact tgtcggtgaa acccagccaa gcgttcttga
     1141 cgttggagaa ctcctcgttc ttcttctcga catccttgta ggttatctcc ttcaagccct
     1201 ggtctccgat cacgccgatc aatccctcgt gcagaatgta atagccgagc gtgggcggcg
     1261 tgccgtggcg cgagatcagc gcgaacggat tgagcgcgac ggtagcctct cccttgttgg
     1321 tcacttcgtc cttgacggtg aaaagatact tgtcgtcgac cgcgatggtg cggcggaaga
     1381 taagtccttc gccgttgtcc cagacgagcg tcaccgggtg tccgacgccg aggccgcccg
     1441 atccctcctg ggtccagacg gtatcggagc ccggcaccct ggtgtcgccg gtcgatttca
     1501 gccaaccgaa ctcggcgtaa taaggatgcg gactgccgga gggcgacagc aagacgatgg
     1561 cgggcgagcg cggatcgacc gtctcccgat attggatgag ggacagatcg tcgatgcggg
     1621 caccggtgag cgcgatcgac ccggaaagac gaggcgtatc gatcgcaatc cgcttcgatt
     1681 tggcgagcgc gtcttcgcgc gaaagctgcg cttgcggcgc agcgcttggc ggcccaggca
     1741 gttgcggcgt cccgcccggc gccggcggac ggctctcggg cgcgccttgc ggctgctgcc
     1801 cttgctgctg ctgggcgatt tgtcgctgct tctccatctg cggcagcccg acgaaatact
     1861 gccacgccag caataccagc gcggagagca cgatggcgag aatcatgttc ttctgatcgt
     1921 tcatgccagg ctctcgtcac ccgcccgcgc gccgatggta tgggcgtggc ccgccgtgca
     1981 gcctttcgag cgcaccctcg aactcggcga tcaatcgatc gaacggcagc gccagcgcgg
     2041 cgcgccgccc gaccaagaca taatcatatc ccgcccgcat gcgttccgcc ggggcgagcc
     2101 gcaccatctc cttgagccgg cgccgcactc ggtttcgttc cgtcgccttg ccgaccttct
     2161 tggccaccgt gaacccgaac cgcggcgggc cgacgtcgtt gcgtcgcagt ccctgcaaca
     2221 cgaatgccgg ggttggttgc ctcgccccgg tcgcgacggc gaggtagtcc gtccgttgcc
     2281 tcagtcgctc catcggaacg aaggggcccg gttgcggccg aattacgcgc tcagt
//
LOCUS       DATNOI010000229        11611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_359318,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000229 DATNOI010000000
VERSION     DATNOI010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11611
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359318"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VN968_08710"
     CDS             complement(<1..203)
                     /locus_tag="VN968_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXL29356.1"
                     /translation="MSEWIGVTIALVSSCLGGSAAAITRYLVGNADPITLAILRWGIG
                     FACLLPTALLLRVRWPPRRDWLAV"
     gene            287..1114
                     /locus_tag="VN968_08715"
     CDS             287..1114
                     /locus_tag="VN968_08715"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL29357.1"
                     /translation="MSRHQGFADSLRRWRRHRGLSQLDLAGRTEISQRHLSFMELGRA
                     SPSRDMVMRLAAALDVPLRQHNALLAAAGFAPVWRQTDLAAPELREIRKALDFMLAQQ
                     EPFPAVAVDRHWNLLQSNSGAVRLIEFLVGPLAPGAPVNLADALVAPDVLRPYLVNWA
                     DVVRYFIRSVEADAAADGTPETAALYERLLGYQGVRAAINAAPTEPATMPVLPMHFRK
                     GNTSLRLFTTIATLGIPLDITLQEMRIECFFPTDDETATIMRRWNAEQRSAAPQASA"
     gene            complement(1187..2272)
                     /locus_tag="VN968_08720"
     CDS             complement(1187..2272)
                     /locus_tag="VN968_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXL29358.1"
                     /translation="MQQVEVAVIGTGWCGGIRAETLSRSSIVDKLHICEIRRDRLAEV
                     KQLTNAATATSDYHDILNNDRIKVVYISTTPESNHYPIARDCLKAGKHVLLEKPIALE
                     LFEADELIEIARRGNLKFTIGYSQRFNTKLAYARKKIADGTLGKPVSVMVSRHLSRGL
                     GQKIASRGRLSPAAMESTHDLDFVFWLLEPARPIRVYSQGAYGYMEAVNGSYDIMWST
                     VTMDNGVLVSIGGGWNLPPSYPNYSATTIEITGTEGSLFLDDTQRSNWLNTMQEGTHY
                     PMSTMPGEWVDHVYAGQMAPETMHFLECVLRDQPPMVSPESARRVMEAYLAADLSAES
                     GEPVSLPLDNQALSAVQELYRKFRTPV"
     gene            complement(2394..3407)
                     /locus_tag="VN968_08725"
     CDS             complement(2394..3407)
                     /locus_tag="VN968_08725"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL29359.1"
                     /translation="MTQGLRRQRNGIGIAAALGSALFLLFGAGASAQDADNYPHRPVM
                     MIVPFAPGGASDFVARIIQHGVGEILGQQIVVDNRPGAAGIVGTEVAARAAPDGYTVF
                     LGNIGTLSINPGVYSNMRIKPDKDLTPVTICADTPSILITRPDFPANTVSELIAYAKA
                     NQGKVVFASPGSSTLNRLEMEVFKKEAGLDLVHVPYKGGAGPAVTDVLGGHVDLMFTT
                     ISSAMQFVKEKQVKALAVTTRERMADLPDIPTMYELGWKNLVTSSWQGVLVPSGTPRP
                     IVDKLHAAIVKVLAEPEVQARMRKGGVIAVGSKSPEDFKTYMDVETAKWTKVIEENGL
                     HSD"
     gene            3944..5545
                     /locus_tag="VN968_08730"
     CDS             3944..5545
                     /locus_tag="VN968_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29360.1"
                     /translation="MKTQSGRILFAAGLITAALASGATGQQDEKLGKVVFPTSCDPNV
                     QAEFDRGVAMLHSYWFLIARRKFEAILQQDPTCAMAYWGVAMDYLGNTLATTPTRADS
                     QAAWDALEKARAAGAKTPRERDWIEALSAYFRDHDKVAVDVRLAAYNAAMERMAQNYP
                     DDYEVQVYYALTLQASAPKSDLTYANQIKSAALLEKLFEQNPQHPGVTHYLIHAYDFA
                     PLAEKGIASARRYAGIAPAVPHARHMPSHIYSMVGLWEESIASNASALEIQPDYYHAS
                     DFTVYAHLQLAQDAKADAMIQKSLATADRGDRPINFVNFTAKAAMPARYVLERADWAG
                     AAALPMTPTQYPMADSLIRFTRGLGMARTGNLAGAKEEIEAMKVLRTTLQRADQSYWA
                     DRTEEQMLAISAWVALKEGAPDQALRFMRAAADSEDGSLKHVAMENRLYPFRELLGEL
                     LLEMGQPGAALNEFETALKQTPNRFRAFWGAARAADSAGDRQKAAEYFGKLVDLARNA
                     DTERQEIREAKAYSRRDETIGGARK"
     gene            5598..6182
                     /locus_tag="VN968_08735"
     CDS             5598..6182
                     /locus_tag="VN968_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL29361.1"
                     /translation="MIRAIALATMLGALVAGVAVYAINPRGWRNGAAPLFGALDHGAK
                     TAIMPEDWPICTTMASVASDAEWAQLDPDFKAGKKALGAQDWSGAIAAFELAALRDPL
                     NADIQNYIGYAYRRLRQLGPAIGHYQQALMLKPRHRSAHEHLGEAYLVLGKPDKAEQL
                     LAALENLCLIPCEESDDLKRAIAAYKRLATARAR"
     gene            complement(6393..7205)
                     /locus_tag="VN968_08740"
     CDS             complement(6393..7205)
                     /locus_tag="VN968_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HXL29362.1"
                     /translation="MAAVLLAAALSVNPGHAQSEKPGVEKLYILNCGEGVAGDISRWS
                     PGVNEGKSMDFVDTCYLIKHAQGWFLWDTGLSDAVAAMPNGLAPSDPRAVYWHRPKTL
                     AAELDQLGVKPADIKAMAISHTHPDHIGNVEMFPTTMLYVQKAEYEWPGANNEPRFKP
                     AHPVTKLEGDHDVFGDGSVTLLSTPGHTPGHQSLLVKLPHTGALVLSGDAVHFKSNWD
                     NRGVPSNNFSKEQTLASMQKISDTLAKEKAQLWINHDKAQRDGLKMSPEFYD"
     gene            complement(7347..7796)
                     /locus_tag="VN968_08745"
     CDS             complement(7347..7796)
                     /locus_tag="VN968_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29363.1"
                     /translation="MHAFAAAGAGFLLAVLWFDLMFDVQTRKPVGDLLPGEVLASISS
                     YYRRVTTEAYPMNRLVALVMVLTLAAICAEIVLGENRWWIGWGSLALAGSGFVLAITR
                     TVPNAVRLGRGNDSPEQQSTLARAIYRDHLLSFGCMMVVLGLQLTAR"
     gene            8066..8884
                     /locus_tag="VN968_08750"
     CDS             8066..8884
                     /locus_tag="VN968_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HXL29364.1"
                     /translation="MSSHTSISQDKLARLIGTAKTPVLIDVRTDEDFAADPRLIPGAV
                     RRSHHDAADWSREYSGGAAIVVCMRGAKLAQGTAAWLRHAGVSAETLEDGFEGWKAAK
                     LPLVPASKLPARDAQGRTVWVTRARPKIDRIACPWLIRRFVDPNAVFLFVTPAEVVAV
                     GERFNAVPFDIENVFWSHRGELCTFDVMIEEFGLATPPLLRLATMVRGADTARLDLCP
                     EAPGLLAASLGLSRMYDDDLEQLEAGIALYDAFYRWCRDATGETHNWPTNKAKP"
     gene            8881..10275
                     /gene="chrA"
                     /locus_tag="VN968_08755"
     CDS             8881..10275
                     /gene="chrA"
                     /locus_tag="VN968_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HXL29365.1"
                     /translation="MIDIAANGTSAEADAGHGISFGEAFRVWLRVALLSFGGPAGQIA
                     VMHRILVEEKNWISESRFLHALNYCMLLPGPEAQQLATYIGWLLHRTAGGIMAGGLFI
                     LPGVIAIMGLSYIYAAFGHVGVIEALFFGLKAAVLAIVVQAVVRVGKRALRNGVMIGL
                     AAAAFIAIFFFNVPFPIIIISAGVIGYVGAKSGRPEFAAVPHGGGKSTAAVDSMLGDE
                     LPDHVRPSVPRALRVSAIWLLLWLVPVAALLITFGEANVFSQIALFFSKMAMVTFGGA
                     YAVLAYVAQQAVEHYHWVQPREMLDGLGMAETTPGPLIMVLQFVGFMAAFRDPGTLPP
                     MLAATLGGLLATWVTFTPCFLWIFLGAPFVETLRGNKGLAGALTAITAAIVGVILNLS
                     IWFALHTVFRETAPVRSFGLSFDMPVISSVDVAALVLAIAAATAIFRFNMGMLTVLGG
                     SCAAGVLLRLAGMI"
     gene            complement(10406..>11611)
                     /locus_tag="VN968_08760"
     CDS             complement(10406..>11611)
                     /locus_tag="VN968_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29366.1"
                     /translation="GVETWEKDSWKEAGNANVWAPMSADEELGTIYLPVSTPTNDYYG
                     GHRPGDGLYGESLVCLDAATGKKVWHYQLVHHGLWDYDSPAAPNLIDITVAGKPIKAV
                     AQVTKQAFVYVFDRVTGQPVWPIEEQPVPASSVPGESASKTQPFPTKPAPIDIQGVRD
                     EDLIDLTPEIHQEAMDIAAKYDHGPLFTPPSERGTIQVPGIGGGANWSGAAIDPDTGM
                     LYVGTYRLPFVVALHKPQAWEGSYDFVASPRYLPGPRGLPLLKPPFGSILAIDMNSGE
                     HRWRIPVGHGETMGAIKNLGIRDQLGFPSRSWALATKTVLIVVQMGYYGPTHFVPELQ
                     RPMADLNNRDPHLWVYDKASGEMLAEIALPANATGAPITYMAGGKQYIAFPVGGGPLV
                     EEELIAVSL"
BASE COUNT         2085 a         3669 c         3649 g         2208 t
ORIGIN      
        1 acggcaagcc agtcgcgacg cggcggccat cgcactcgaa gcaaaagcgc cgtcggcagc
       61 aaacatgcaa aaccgatgcc ccagcgcaga atggcaagcg tgatcggatc ggcgttgccg
      121 accagatagc gcgtaatcgc cgccgcgctg ccgccgaggc agctcgagac caatgcgatg
      181 gtcacaccga tccattcact caccgcccgt ccccccgtgt cgcgctgcgc ttgtttgtgg
      241 catgttgccg aatggggtcg tttcagcaac tacctggcag gtactcatgt ctcggcatca
      301 gggcttcgca gacagcctgc gccgctggcg ccggcaccgc ggcctgtcgc agctcgatct
      361 ggccggacgc acggaaatct cgcagcgcca tctcagcttc atggaattgg gccgcgcatc
      421 gcccagccgc gatatggtga tgcggctggc ggccgccctc gacgtgccgc tgcgccagca
      481 caatgcgctg ctggcggctg ccggctttgc cccggtctgg cggcagaccg acctagccgc
      541 acccgaactt cgtgagattc gcaaggcgct cgacttcatg ctggcccagc aggagccgtt
      601 tcccgccgtc gcggtcgacc ggcactggaa tttgctgcag agcaattcgg gcgcggtacg
      661 cctgatcgaa ttcctggttg gaccattggc gcccggcgcg ccggtcaatc tggccgatgc
      721 gctggtcgca ccggatgtgc tgcggccgta tctggtgaac tgggcggacg tcgttcgcta
      781 cttcatccgc agcgtggaag ccgacgccgc cgccgacggc acgcccgaga cggcggcgct
      841 ttacgaaaga ctgctcggct atcaaggggt gcgggcggca atcaacgccg cgccgaccga
      901 accggcgacc atgccggtgc ttccgatgca tttccgcaag ggcaacacca gcttgcgatt
      961 gttcaccacc atcgcgacgc tcggtattcc cttggacatc acgctgcagg aaatgcgcat
     1021 cgaatgcttt tttccgaccg atgacgaaac cgccacgatc atgcgccggt ggaatgccga
     1081 gcaacgatcc gccgcaccgc aggccagcgc gtgaacgggt agatgcgctg ggccttgcgg
     1141 atggttcatg gccctgaggg gcgagcccag gcggcgccgc gactggctag accggcgtgc
     1201 ggaacttcct gtaaagctcc tgaaccgcgg agagcgcctg gttgtcgagc ggaaggctga
     1261 cgggctcgcc ggattccgcc gagagatccg cggcgaggta ggcctccatc acgcggcgcg
     1321 cgctttccgg cgacaccatc ggcggctggt cccgcaatac gcattcgagg aagtgcatcg
     1381 tctcgggtgc catctgcccg gcgtagacgt gatcgaccca ctcgcccggc atggtcgaca
     1441 tcggatagtg cgtcccttcc tgcatcgtat tgagccagtt cgagcgctgg gtatcgtcca
     1501 ggaacaggga gccttccgtg ccggtgatct cgatcgtcgt ggcgctgtag ttcggatagc
     1561 ttggcggcag gttccagccg ccgccgattg aaaccagcac gccattgtcc atggtcacgg
     1621 tgctccacat gatgtcatag gagccgttga cggcctccat gtagccgtag gcgccctgcg
     1681 aatagacgcg gatgggcctc gccggctcga gcagccagaa cacgaaatcg aggtcgtggg
     1741 ttgattccat cgccgccggc gacagccggc cgcggcttgc gatcttctgg ccgaggccgc
     1801 gcgacagatg ccggctcacc atgaccgaca ccggcttgcc cagcgtgccg tcggcgatct
     1861 tcttcctggc gtaggcgagc ttggtgttga agcgctgcga gtagccgatg gtgaatttga
     1921 gattgccgcg tctggcgatc tcgatcagct cgtcggcctc gaacagttcg agcgcgatcg
     1981 gcttttccag gagcacgtgc ttgccggcct tgaggcaatc gcgcgcgatc ggatagtggt
     2041 tggattccgg cgtcgtcgag atgtagacca ccttgatccg gtcgttgttg aggatgtcgt
     2101 gatagtctga tgtcgcggtt gccgcgttgg tcagctgctt gacctcggcc aggcggtcgc
     2161 gcctgatttc gcagatatga agcttgtcga cgatcgacga gcgcgacagc gtttccgccc
     2221 gaatgccccc gcaccagcca gttccgatca ccgcgacttc gacctgttgc acgcttgtgt
     2281 ctcctgtttt ccactatcga attccggcga atatggcttt ggccatgccg atacccggtg
     2341 ccgtgcatag aaaggacctt tcacccgcgc cgcgtcaacc ggaacgcagc ggctcaatcc
     2401 gaatgcagcc cgttctcctc gatcaccttg gtccacttgg cggtttcgac gtccatatag
     2461 gtcttgaagt cctcggggga cttgctgccg accgcgatca cgccgccctt gcgcatgcgc
     2521 gcctgaacct ccggctcggc gagcaccttg acaatcgcgg cgtgcagctt gtcgacgatc
     2581 ggccgcggcg tgcctgatgg cacgagcacg ccctgccagg atgacgtcac gaggttcttc
     2641 catccgagct cgtacatggt cggtatgtct ggcagatcgg ccattcgctc ccgggtggtg
     2701 acggcgagcg ccttgacctg cttttccttc acgaactgca tagccgagga gatggtggtg
     2761 aacatgaggt ccacatgtcc gccgagaacg tccgtcaccg ccgggccggc gccgcccttg
     2821 taggggacgt gcacgaggtc gaggcctgcc tccttcttga aaacctccat ctccagccgg
     2881 ttgagcgtgc tgctgccggg cgaggcaaag acgaccttgc cctggttcgc cttcgcataa
     2941 gcgatcagct cgctgacagt gttggccggg aagtccggcc gggtgatcag gatgcttggc
     3001 gtgtcggcgc aaatcgtaac cggggtcagg tccttgtccg gcttgatacg catgttggag
     3061 tagacgcccg ggttgatcga gagggtaccg atattgccga gaaagacggt gtagccgtcc
     3121 ggcgccgcgc gggccgcgac ctcggtgccg acgatgcccg ccgcgcccgg ccgattgtcg
     3181 accacgatct gctggccgag gatctcgccg acgccgtgtt ggatgatgcg ggcgacgaaa
     3241 tcggaagcgc cgccgggcgc gaacggcacg atcatcatga cgggtcgatg gggatagttg
     3301 tcggcgtcct gggcggaggc tcctgcgcca aacaggagga acagtgcgct gccgagcgcc
     3361 gccgcgattc caattccatt tcgctgccgg cgcagaccct gggtcacgtt caaaggcttt
     3421 tctccctgac gatcggcggg cgcttgatgc acctctgact tcgatcataa caacgggagt
     3481 tgaggtcgca agcgccaagg ggtgcgggcg gccgtctgcg ccggtgcgcc gtgacctcct
     3541 gtgcatcgat ttgacgatat ccgtgtagct tccgcttatc cccccgatag cggcggtatt
     3601 gcgaacgtcg cggcacgtcg catttgtgcc aagcacggaa gtccccggct ctttcaggcg
     3661 cacgggaagt ggtgtaatct tcaatcggcg cgctgccaat gggactgccg aaatcccgca
     3721 aatttaagga cagcgcctgt gccttcggcg ggcaagcatt gtgcaattca ttcgatattg
     3781 ggtttgcggg ccttggaccg atgcgtatgt ccgcatcgca atgccttttg caggacaacc
     3841 gcgcaaacag tcaaatctgg agccaaagga atagacggag acgtatccgt ttaatggagc
     3901 aagctcacgg gccatcgccc gcaagccaga gaggagattt acgatgaaga cgcaatcggg
     3961 gcgaattttg tttgccgctg gcctgatcac cgcggcgctc gcaagcggcg caaccggcca
     4021 gcaagacgag aagctcggca aagtggtctt ccctacctcc tgcgatccca atgtgcaggc
     4081 cgagttcgac cgcggcgtcg ccatgctgca ttcatactgg ttcctgatcg cccgccggaa
     4141 gttcgaggcg attctacagc aggatcccac ctgcgcgatg gcctattggg gcgtggcgat
     4201 ggactacctc ggcaacaccc tggcaaccac gccgacgcgg gccgactcgc aggcggcctg
     4261 ggacgcactc gaaaaggcgc gggcggccgg ggcgaagacg ccgcgcgagc gcgactggat
     4321 cgaggcgctc agcgcctatt tccgcgacca tgacaaggtc gccgtggatg tccgactggc
     4381 ggcctacaat gccgcgatgg agcggatggc gcaaaactat cccgacgact acgaggtgca
     4441 ggtctattat gcgctgacgc tgcaagcctc ggcgccgaaa agcgacctca cctacgccaa
     4501 ccagatcaaa tccgcagcac tgctggaaaa actcttcgag cagaacccgc agcatccggg
     4561 tgtgacgcac tatctcattc acgcctacga ctttgcgccg cttgccgaaa agggaattgc
     4621 ctccgcgcgg cgctatgccg gcattgcgcc ggccgtgccg cacgcgcggc acatgccatc
     4681 gcacatctac tccatggtcg gactttggga agaatccatc gcctcgaacg cttcggcgct
     4741 tgagatccag ccggactact atcacgcgtc ggattttacc gtctatgcac atctgcaact
     4801 cgcgcaggat gccaaagccg atgcgatgat ccagaagtct ctcgccaccg ccgaccgcgg
     4861 tgatcgcccg atcaacttcg tcaatttcac cgccaaggcg gctatgccgg cgcgctatgt
     4921 cctggaacgg gcggactggg ccggcgctgc cgcgctgccg atgactccca ctcaatatcc
     4981 catggcggat tcgctgatcc gcttcacgcg cggtctcggc atggcgcgca ctggcaatct
     5041 cgcaggcgct aaagaagaga tcgaagcgat gaaggttttg cgcacgactc tgcagcgagc
     5101 cgaccagtcc tactgggccg accgcaccga agaacaaatg cttgccattt ctgcgtgggt
     5161 cgccttgaaa gaaggcgcgc ccgatcaggc cctgcggttc atgcgggcag ccgccgacag
     5221 cgaggacggt agtctcaagc acgtggcgat ggagaatcgc ctctatccgt tccgcgaact
     5281 gctgggggaa ttgcttctcg agatggggca gcccggagcc gccctgaacg agttcgagac
     5341 cgcgctcaag caaaccccca accgcttccg cgcgttctgg ggagccgccc gtgctgccga
     5401 cagtgcgggc gaccgccaga aggcggccga gtacttcggc aagctggtgg acttggccag
     5461 gaacgctgat acggagcggc aggaaatccg tgaggccaag gcatactcgc gccgggatga
     5521 aaccataggt ggtgcacgaa aatgacgcct cggctgccgg gtctagcacc cggcagccgt
     5581 caacccgaga gacaacgatg atccgcgcga tcgcattggc tacaatgctc ggtgcgctcg
     5641 tcgccggcgt ggccgtctac gccataaacc cgcgggggtg gcggaacggg gctgcaccgc
     5701 tgttcggtgc gctcgaccat ggggccaaga cggcaatcat gcccgaagac tggccgatct
     5761 gcacaaccat ggcatcggtc gcatcggatg ccgaatgggc gcagctcgac cccgacttca
     5821 aggccggcaa gaaggcattg ggtgcgcagg actggagtgg tgcaatcgca gcgttcgaac
     5881 tcgccgcact gcgcgacccg ctcaatgccg atatccagaa ttacatcggc tatgcctatc
     5941 ggcggctacg tcagctgggg ccggccatcg gccattatca gcaggcgctg atgctgaaac
     6001 cgcgccaccg cagcgcgcat gagcatctcg gcgaggccta tctggtgctc ggcaagcccg
     6061 acaaggccga gcaactgctg gcagcgctgg aaaacctctg cctcattccc tgcgaggaat
     6121 ccgacgacct caagcgggcc attgcggcct acaaaaggct ggccacagcc agagcgcgat
     6181 gacttttctt cgaatcgtca tcccgctcta tctttttgtt tgagcatgat cttttcggaa
     6241 aaccgctaac cacttttccg gatcatgctc taggatcaga tcaggcgccg taagagcgtt
     6301 gcaacaagcg ctggaaatca tggcatcttg cgcttggtcc gtttctacgg ccgcgcaatg
     6361 gccggaccaa tccgccggta acgcacgcct cctcaatcgt aaaactccgg cgacattttc
     6421 aggccgtcgc gctgcgcctt gtcgtggttg atccagagct gcgctttttc cttggcaagc
     6481 gtgtcggata tcttttgcat cgaggccagg gtctgctcct tgctgaagtt gttggaggga
     6541 accccgcggt tgtcccagtt gcttttgaaa tgaacggcat cgccggacag aacgagcgcg
     6601 ccggtatgcg gcagcttcac cagcagggat tgatgacccg gcgtatgtcc gggggtcgaa
     6661 aggagggtga cgctaccgtc gccgaatacg tcatggtcgc cttccagttt tgtcacggga
     6721 tgcgcgggct tgaaccgggg ctcgttgttg gccccgggcc attcgtattc ggctttctgg
     6781 acgtagagca ttgtggtcgg aaacatctca acattgccga tgtgatcggg atgggtgtgc
     6841 gaaatggcca tcgccttgat gtcggcgggc ttgacgccaa gctggtcaag ctctgccgcc
     6901 aatgtcttcg ggcgatgcca gtagacggcc cttggatccg atggagcaag gccgttcggc
     6961 atcgccgcga ccgcgtccga tagcccggtg tcccacagga accagccttg cgcgtgcttg
     7021 atcagatagc aggtgtcgac aaagtccatc gatttgcctt cgttgacccc aggcgaccag
     7081 cgcgaaatat cgccggcgac gccttccccg caattgagga tgtaaagctt ttcgacgccg
     7141 ggcttttccg attgcgcatg gccgggattg accgacaagg ccgctgccaa aaggaccgcc
     7201 gccagaccac cacctgcttg tttcacgtca tttctccaac gccgacagac ccgaaatttc
     7261 cccgatgggc tggatagtac tgcatagcca atgttgcatc cagacgacgc ctggcgcgaa
     7321 aggtctggcg cgaagaccag gcgccatcat cgcgccgtca attgcaggcc aagaaccacc
     7381 atcatgcacc cgaagcttaa gagatgatca cgataaattg cgcgcgccag cgtcgattgc
     7441 tgctccggtg aatcattgcc gcggccgagc cggaccgcgt tcgggaccgt gcgggtgatg
     7501 gcgagcacaa agccgctgcc ggccagcgca agcgaacccc agccgatcca ccagcggttt
     7561 tcgcccagca cgatctccgc gcagatcgca gccagcgtca gcaccatcac cagggcgacc
     7621 agccggttca tcggataggc ctccgtcgtg acgcgtcggt aataggacga aatcgaggcc
     7681 agcacttcgc ccggcaagag atcgccgacg ggcttgcggg tctgcacatc gaacatcaga
     7741 tcgaaccaca acacggcaag caggaaaccg gcccccgccg cggcgaatgc atgcatggcg
     7801 aagcccccct tcttcgcggc catcataatt catcatcggc ccgatgacgg gagcctggct
     7861 tgcgtatcgc gccgtcagcg gcggcgggtg tgaacaaggc gttacgcgcg gcgcgaactt
     7921 ctaccggctc ccgccggcaa ggccaacgac tggattgaca gcccgcgtca gtttcgctgt
     7981 aatggcgctc tgggggttct gcgatccccc cacgaggcga catgcccaag tatcgcgtcc
     8041 cgtttctttt cacgagggcg cattcatgtc atcccacacc tccatatccc aggataaact
     8101 tgcccgtctg atcggcacgg cgaaaacgcc cgtcttgatc gacgtccgaa cggacgagga
     8161 ttttgccgcc gacccgcggc tgattccagg tgcggtcagg cgcagccatc acgacgccgc
     8221 cgactggagc cgcgaatatt ccggcggcgc tgctatcgtc gtctgcatgc gcggcgcaaa
     8281 actggcgcaa ggcacggccg cctggttgcg gcatgccggt gtatcagcgg aaacgctgga
     8341 agacggtttc gaaggctgga aggcggcaaa actgccgttg gtgccggcgt caaaactgcc
     8401 cgcgcgcgat gcgcagggac gcacggtctg ggtaacccgc gccaggccga agatcgatcg
     8461 catcgcctgc ccctggctga tccggcggtt cgtcgatccg aatgcggtgt ttctgtttgt
     8521 gacacccgcc gaggtcgtcg cggtcggcga acgcttcaat gccgttccct tcgatatcga
     8581 aaacgtgttc tggagtcatc gcggtgaact ctgtaccttc gacgtgatga tcgaggagtt
     8641 tggcctcgcg acgccgccct tgctgcggct ggcgaccatg gtacgcggcg ccgataccgc
     8701 gcggctcgat ctctgtccgg aagcgccggg gttgctcgcg gcgtcactag gcctgtcgcg
     8761 gatgtatgac gacgatctcg aacaactcga ggccgggatc gcgctgtacg atgcgttcta
     8821 tcgctggtgc cgcgatgcga ccggcgaaac ccataactgg cctactaaca aggcaaagcc
     8881 gtgatcgata tcgccgccaa cgggacctca gccgaggctg atgccggtca cggcatcagc
     8941 ttcggcgaag cctttcgggt ctggctccgg gtcgccctat tgagtttcgg cggcccggcc
     9001 ggacagatcg cggtgatgca ccgcatcctg gtcgaggaaa agaactggat ttccgagagc
     9061 cgattcttgc acgcgctgaa ctattgcatg ctgctgccgg ggccggaggc gcagcagctt
     9121 gcgacctata tcggctggct cttgcaccgc accgccggcg gcatcatggc cggcggtctt
     9181 ttcatacttc ccggcgtcat cgccatcatg ggcttaagct acatctatgc cgccttcggc
     9241 catgtcgggg tcatcgaggc gctgttcttc ggcctcaagg cagccgtgct ggcgattgtc
     9301 gttcaggccg tggtccgcgt cggaaaacgc gcgctgcgca acggggtcat gatcgggttg
     9361 gcagccgcag ccttcatcgc gatcttcttt ttcaacgtgc ccttcccgat catcatcatc
     9421 tcagccggcg tgatcggcta tgtcggcgcc aagagcgggc ggccggaatt tgccgctgtt
     9481 ccgcatggcg gtggcaaaag taccgccgcg gtcgacagca tgctcggcga tgaattgccg
     9541 gaccatgtcc gcccgagcgt tccgcgcgcg ctaagagtaa gcgccatctg gctgctgcta
     9601 tggctggtgc cggtcgcagc ccttctgatc acgttcggcg aggccaacgt gttcagccag
     9661 atcgcattgt tcttcagcaa gatggcgatg gtgacgttcg gcggcgccta tgcggtgctg
     9721 gcctacgtcg cgcagcaggc ggtcgagcat tatcactggg tgcagccgcg cgagatgctg
     9781 gatggcctcg gcatggcgga gaccacgccg ggcccgctga tcatggtgct gcagttcgtc
     9841 ggcttcatgg ccgcgtttcg cgatcccggc acgctgccgc cgatgctggc ggccacgctc
     9901 ggcgggttat tggcgacctg ggtcaccttc acgccctgct ttctctggat ctttctgggc
     9961 gcgccgtttg tcgagaccct gcgcggcaac aaggggctgg cgggcgcact gaccgcgatc
    10021 actgcggcca ttgtcggcgt gatcctcaat ctgtcgatct ggttcgcgct gcataccgtg
    10081 ttccgggaaa ccgcgccggt gcgatcgttc gggctgtcgt tcgacatgcc cgtgatatcg
    10141 agcgtcgatg ttgccgcgct cgtcctggcc atcgcggctg cgaccgcgat ttttcgattc
    10201 aatatgggca tgctgacggt gctgggaggt tcgtgcgccg cgggcgtgct gctgcggctg
    10261 gcggggatga tctgatctct cgtcgtcccg gcgaaggccg ggacccataa ccccgggcgt
    10321 tgattgttgc agaaggtctc ggcaccggtg ccgaaacgag aatcctcggc gtatgggccc
    10381 cagcgttcgc cggggcgacg ccaactcaaa gcgaaaccgc gatcagttcc tcctcgacca
    10441 gcggaccgcc gccgacgggg aacgcgatat attgcttgcc gccggccatg taggtgatcg
    10501 gcgcgccggt cgcgttggcc ggcagcgcga tctccgccag catttcgccg ctggccttgt
    10561 cgtagaccca cagatgcgga tcgcgattgt tgagatcggc catcggacgc tggagctcgg
    10621 gcacgaagtg cgtcggcccg tagtatccca tctgaaccac gatcaagacc gtcttggtcg
    10681 ccagcgccca gctgcgggac ggaaacccca gctgatcgcg aatacccagg ttcttgatgg
    10741 cgcccattgt ctcgccgtga ccgacgggaa tgcgccagcg atgctcgccg ctattcatgt
    10801 cgatggcaag gatgctgccg aacggaggct tgagcagcgg caatccgcgc ggccccggca
    10861 aataccgggg gctcgcaaca aaatcgtagg aaccttccca ggcttgcggc ttgtggagtg
    10921 cgacaacgaa cggcagccgg taggtgccaa catagagcat gccggtgtcg ggatcgatcg
    10981 cggcacccga ccagttcgcg ccgccaccga tgccgggcac ctggatcgtg ccgcgctccg
    11041 acggcggcgt gaacagcggg ccgtgatcat atttggcagc gatgtccatc gcctcctggt
    11101 ggatctccgg cgtcaggtcg atcaggtcct cgtcgcggac gccctggatg tcgatcggcg
    11161 cgggttttgt gggaaacggc tgtgtcttcg acgcgctctc gccgggcacg ctcgacgccg
    11221 gcaccggctg ctcctcgatt ggccagaccg gctggccggt gacgcggtcg aatacataca
    11281 caaaagcctg ttttgtgact tgcgcgaccg ccttgatcgg ctttcccgcc acagtgatgt
    11341 cgatcaggtt gggagccgcc ggcgagtcgt aatcccacaa cccgtgatga acgagctgat
    11401 agtgccagac cttcttgccg gtcgccgcgt cgagacacac gagactctcg ccgtagagac
    11461 cgtcgccggg gcggtggccg ccgtaataat cattggtcgg cgtgctgacc ggcaagtaga
    11521 tggtgccgag ctcctcgtcg gcgctcatcg gcgcccagac attggcattg ccggcttcct
    11581 tccaggaatc cttctcccag gtctcgaccc c
//
LOCUS       DATNOI010000230         4712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_196576,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000230 DATNOI010000000
VERSION     DATNOI010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196576"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VN968_08765"
     CDS             complement(<1..788)
                     /locus_tag="VN968_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL29367.1"
                     /translation="MQTLTRSGTLRIGASDLEYRMIGPAPDEAPTIVMLHEGLGCAAL
                     WGDFPDRLAAATGAGVFVYSRAGYGASTKVALPRPLDYMHVEALDVLPKLLDKVGFRR
                     GLLLGHSDGASIAAIYAGGVQDHRVRAVAMIAPHFIVEDIGLTSIAETRKAYEAGGLK
                     EKLKRWHRDVDNTFYGWNDAWLDHRFRSWDISEYLAYIRVPLAILQGADDQYGTIRQI
                     EIAQEECYCPVEVTMIPGAGHSPHREAPEPTLDTISEFVERILY"
     gene            complement(982..2517)
                     /locus_tag="VN968_08770"
     CDS             complement(982..2517)
                     /locus_tag="VN968_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HXL29368.1"
                     /translation="MSAYNAVTWLLDRNVDEGRGGKLAFTDTVSELTYGDLQKQSCRV
                     ANLLRRLGVHREERVAMIMLDTVDFPAVFLGAIRAGVVPVPLNTLLTSDQYAYVLADC
                     RARVLFVSEALLPVVKDMVGRMADLDHVIVAGNDAHGYKKLSDELAGESSSFATAATH
                     ADEPAFWLYSSGSTGMPKGVRHLHSNLAATAETYARQVLGIREDDVGLSAAKLFFAYG
                     LGNALTFPMSVGATTVLNSERPTPAVMFALMNRYNPSIFFGVPTLFSAMLNDETQNNE
                     RCGSRLRICTSAGEALPESVGNSWKSRFGVDILDGVGSTELLHIFLSNAPGDIKYGSS
                     GRPVPGYRVRLVNEAGSDVPDGEVGELLVDAPSAGEGYWNQRSKSRQTFEGHWTRTGD
                     KYIRDADGRYTFCGRSDDMFKVSGIWVSPFEVESALITHPAVLEAAVVPEADPEGLLK
                     PKAFVVLRADADTDGLHEALKEHVKQKIGPWKYPRWIDVVDSLPKTATGKIQRFKLRD
                     GAT"
     gene            2604..3545
                     /locus_tag="VN968_08775"
     CDS             2604..3545
                     /locus_tag="VN968_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL29369.1"
                     /translation="MKPASQPDRTTRDMTENLDLETGFLEQLGQRVRTMRALRGMSRK
                     VLAKVSGISERYIAQLESGKGNVSIVLLRRVSNAMGAHLEDLIPATEPSPDWPVIRDL
                     VRKATPNQIAQAKDALAGQGNGASAQRRMSFAGIALIGLRGAGKSTLGKMLAKKIGWS
                     FVELNKEIEAQNGLSVAEIIALYGQEGFRRLEQAALGQLLARKELMVLATGGGIVSEP
                     LTFDLILSSFYTIWLKAEPEEHMARVRAQGDLRPMADDRSAMVELRTILRSREPLYAR
                     ASAVVDTAGLSVDDAAARLIDAVAPVLHDEARMFARG"
     gene            3658..>4712
                     /locus_tag="VN968_08780"
     CDS             3658..>4712
                     /locus_tag="VN968_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXL29370.1"
                     /translation="MMSQEQNDLITRTGPKDACGKLMRMYWQPAALVDELTGPRPVKA
                     VKLLGEDLVLFRDEQGRHGLIDRHCAHRGADLAFGRLEHGGLRCAFHGWLFDVSGQCL
                     ETPAEPKGSVLCKNITQRAYPVVEKSGILWTWLGEGEPPAFPEIDCFVAPDSHTFAFK
                     GLIGCNWLQALEVGIDPSHASFLHRFFEDEDPAAAYGKQFRGASAGSDMPMTKILREY
                     DRPIINVEHTEYGLRLIALREIDEERTHVRVTNQLFPHGFVIPMSTEMTITQWHVPVD
                     DENCYWYAIFTSYSTPVDKKKMRDQRLELYELPDYRSRKNKSNDYGFDPHEQLTATYT
                     GMGADINVHDQWAVESMG"
BASE COUNT          927 a         1451 c         1466 g          868 t
ORIGIN      
        1 aaatacaaaa tgcgttcaac aaattctgaa atcgtatcga gcgtcggctc cggcgcttcg
       61 cgatgcggcg aatgtccggc accgggaatc atcgtcactt cgaccggaca atagcattct
      121 tcttgcgcga tctcgatctg acggatggtc ccgtactgat cgtcggcgcc ttgcaggatc
      181 gcgagcggta cccggatgta ggcgagatac tccgaaatat cccagctacg aaacctgtgg
      241 tcgagccacg cgtcattcca accgtagaag gtgttgtcga catcgcggtg ccagcgcttc
      301 agcttctcct tgaggccgcc ggcctcatag gccttcctgg tctccgcgat cgacgtcagc
      361 ccgatatctt ccacgatgaa atgcggcgcg atcatcgcca ccgcgcgcac gcgatgatcc
      421 tggacaccac cggcgtagat cgccgcaatc gaagcgccgt cggaatgccc gagcaacagg
      481 ccgcggcgaa agccgacttt atcgagcagc ttcggcaaca catcgagcgc ttcgacatgc
      541 atgtagtcga gcgggcgcgg cagcgcgacc tttgtcgagg cgccatagcc ggcgcgtgaa
      601 tacacgaaca cgccggcgcc agtggcggcg gcaagccggt ccggaaaatc tccccacagc
      661 gcggcacacc ccaagccttc gtgcagcatg acgatggtcg gcgcctcatc cggcgccgga
      721 ccgatcatgc ggtattcgag atcggaggcg ccgatgcgaa gggtgccgga gcgggttaga
      781 gtttgcatga aattagcttt ccaaaagtaa tgcacatgtc ggaattctca tgcgggtcgt
      841 cccggcgaac gccgggaccc ataacccctg gcgccgatgt tgacagatgg tatctgcccc
      901 accggccaaa acgcgagtcc gcgccgtatg ggtcccggcg ttcgccggga cgactcgtgg
      961 aggcgatcat gcttccctct actacgtcgc cccgtcccgc agcttgaacc gctggatttt
     1021 gcccgtagcg gttttcggca ggctgtcgac gacgtcgatc cagcgcggat atttccacgg
     1081 gccgatcttc tgcttgacgt gctccttcag cgcctcatga aggccgtcgg tgtccgcgtc
     1141 cgctcgcagc accacgaacg ccttgggctt cagcaatcct tccggatcgg cttcgggcac
     1201 cacggcggcc tccagcaccg cgggatgcgt gatcaacgcg ctctcgacct cgaacggcga
     1261 cacccagatg ccggacacct tgaacatgtc gtcgctgcgg ccacagaaag tgtagcgccc
     1321 gtcggcgtcg cgaatgtatt tgtcgccggt gcgggtccag tggccttcaa acgtctgacg
     1381 gctcttgctg cgctgattcc agtagccttc cccggccgag ggcgcatcga ccaggagttc
     1441 accgacttcg ccgtcgggaa catcgctgcc ggcttcgttg acgagccgca ccctgtagcc
     1501 cggcaccgga cggccggatg agccgtattt gatatcgccg ggggcattgg agagaaaaat
     1561 atgcaacagc tcggtcgagc cgacgccatc gagaatatcg acgccgaagc gtgacttcca
     1621 gctgttgccg accgattccg gcagcgcctc gcccgcggag gtgcagatgc gcaagcggga
     1681 gccacagcgt tcgttgttct gggtttcgtc gttcagcatc gctgagaaca aggtcggcac
     1741 gccgaagaaa atgctgggat tgtatctatt catcagcgcg aacatcacag ccggcgtcgg
     1801 acgctcggag ttgaggacag tggtggcgcc caccgacatc gggaacgtca gcgcgttgcc
     1861 gaggccatag gcaaaaaaca atttcgccgc cgatagacca acgtcgtcct cgcgaatccc
     1921 gaggacttgc ctggcgtagg tctcagccgt ggcggcaaga ttggaatgca gatgccgcac
     1981 gcccttcggc atgccggtgg agcccgacga atagagccag aatgccggct cgtcggcatg
     2041 ggtcgcggcg gtcgcgaatg aggagctttc gcccgcgagt tcatcggaca gttttttgta
     2101 gccgtgcgca tcgttgccgg cgacgatgac gtgatcgaga tcggccatcc gcccgaccat
     2161 gtccttgacc acaggcagca gcgcctcgga aacaaacagc acccgcgcgc ggcaatccgc
     2221 cagcacatag gcatattgat ccgaggtcag cagcgtgttg agcggcaccg gcaccacgcc
     2281 ggcgcgaatg gcgccgagaa acaccgccgg aaagtcgacc gtgtcgagca tgatcatcgc
     2341 cacgcgctct tcacggtgga cgccgagacg ccgcagcaga ttggcgacgc ggcagctctg
     2401 cttctgcaga tcgccatagg tcaattcgga aacggtgtcg gtgaacgcga gttttccgcc
     2461 gcggccttca tcgacattgc gatcaagcag ccaggtcacc gcgttgtagg cgctcacgcg
     2521 tcttctcccc agtttttaag aattataatt catagaaagg caccagactc gcttgctgtc
     2581 aatccttgtc ggcattatgt ttcctgaagc ccgcatcgca accagaccga accacgaggg
     2641 acatgaccga aaatctcgat ctcgaaaccg gctttctcga gcagctcggc cagcgtgtcc
     2701 gcaccatgcg tgccctgcgc ggcatgtcgc gaaaggtgct ggcaaaagtt tccggaattt
     2761 ccgaacgcta tatcgcccag ctcgaaagcg gcaagggcaa cgtctcgatc gtgctgctgc
     2821 gccgggtgtc gaatgcgatg ggcgcgcatc tcgaggatct cattcccgca accgagcctt
     2881 cgccggactg gcccgtcatt cgcgatctcg tgcgcaaggc gacgccgaac cagattgcgc
     2941 aggccaagga cgccctcgcc ggtcaaggca acggcgcctc ggcacagcgg cggatgtcgt
     3001 tcgccggcat cgcgctgatc ggcctgcgcg gcgccggcaa atccacgctc ggcaaaatgc
     3061 tggcgaagaa gatcggctgg agttttgtcg agctgaacaa ggagatcgaa gcgcagaacg
     3121 gattgtcggt cgccgagatc atcgcgctgt acggccagga aggttttcgc cggctggagc
     3181 aggccgcgct cggacagttg ctggcgcgga aagagctgat ggtgctggcg accggcggcg
     3241 gcatcgtctc cgagcccctg accttcgacc tgatcctgtc gtcgttctat accatctggc
     3301 tgaaggccga gccggaagaa cacatggcgc gggtgcgggc gcagggcgat ctgcgtccga
     3361 tggcggacga tcgctcggcc atggtcgagc tgcgcaccat cctgcggagc cgcgagccgc
     3421 tttatgcccg ggcgtctgcg gtagttgata ccgcgggcct ttccgtggac gatgccgccg
     3481 cgcggttgat cgacgcggtg gcgccggtgt tgcatgacga ggcccgcatg ttcgcgcggg
     3541 ggtgaacctc gtatcccgaa cgcaatctgg gcaaggaatg tgcttgcggg gaaacgcggg
     3601 gctagtaacc tgatttcgaa tgagatcaat tctgcttcga taaaggaccc cgccgccatg
     3661 atgagccagg aacagaatga cctgatcacc cggaccgggc caaaggatgc atgcgggaaa
     3721 ttgatgcgga tgtactggca gccggcggcg ctggtggatg agctgacggg accgcgcccg
     3781 gtcaaggcgg tcaaattgct cggcgaagat ctggtgctgt ttcgggacga gcaaggccgt
     3841 cacggcctga tcgatcgcca ttgcgcgcat cgcggcgccg atctcgcgtt cggccgtctg
     3901 gagcacggcg gcctgcgctg cgccttccac ggctggctgt tcgatgtctc gggccaatgc
     3961 cttgaaacac cggcggagcc gaagggttcg gtgctgtgca agaacatcac ccagcgcgcc
     4021 tatccggtgg tcgagaagag cggcattctc tggacgtggc tcggcgaagg cgagccgccg
     4081 gcgtttccgg aaatcgactg ttttgtggcg ccggatagcc acaccttcgc gttcaagggc
     4141 ctgatcggtt gcaactggct gcaggcgctc gaggtcggca tcgatccgtc gcacgcctcg
     4201 ttcctgcatc gctttttcga agatgaagat ccggcggctg cttacggcaa gcagtttcgc
     4261 ggcgcatcgg ccggcagcga catgccgatg accaaaatcc tgcgcgaata tgaccgaccg
     4321 atcatcaatg tcgagcacac cgaatacggc ttacggctga tcgcgctacg cgagatcgac
     4381 gaggagcgca cccatgtgcg cgtcaccaat cagctgttcc cgcatggttt tgtcatcccg
     4441 atgagtaccg agatgacgat tacgcagtgg cacgtgccgg tcgatgacga gaactgctac
     4501 tggtacgcga tcttcaccag ctattcgacg ccggtcgaca agaagaaaat gcgcgaccag
     4561 cgcctcgaac tgtacgagct tcccgattat cgctcgcgca agaacaagtc gaacgattac
     4621 ggtttcgatc cgcacgagca gttgacggcg acctacaccg gcatgggcgc cgatatcaac
     4681 gtgcacgacc agtgggcggt cgaatcgatg gg
//
LOCUS       DATNOI010000231         6252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1492260,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000231 DATNOI010000000
VERSION     DATNOI010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492260"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2754
                     /locus_tag="VN968_08785"
     CDS             <1..2754
                     /locus_tag="VN968_08785"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HXL29371.1"
                     /translation="LIDTDGVKGVTSNPSIFEKAIGSSDEYDGAIGKALKKGDRPIAE
                     LFEHLAVEDIQHAADVLRPVYDELKGEDGFVSLEVSPYLAMDTKATIAEAERLWKDVK
                     RKNLMVKVPATPEGLPAIQHLIGEGISINITLLFSQKVYVQVAQAYLAGLEKYVAKGG
                     DPSRVASVASFFVSRIDSAVDKALDEKIARANDPTEKERLAALKGKIAIANAKLAYRD
                     YKRLFSGSRWDKLAAKGAKPQRLLWASTGTKNKDYSDVLYVEELIGPNTVNTVPPATL
                     DAFRDHGTPRDSLEENIEDARGVLAELDTSGISLDAITADLVKDGVKLFADAADKLYG
                     AVAHKRAAVLGAGIDRQEFALGSSIGKAVEKSTEDWRASAKIRRLWQHDQSVWTGTDE
                     NKWLGWLNSAAAADIADYEDFAHRVKGQNFTDAVVLGMGGSSLGPEVLAQTFEKQPGF
                     PKLHVLDSTDPAQVRTLQASINIANTLFIVSSKSGGTTEPNVMKDYFFARVSETIGAD
                     KAGHRFIAVTDPGSSLEKVATRQGFARIFHGDPSIGGRYSVLSPFGLVPAATAGIDVA
                     TLLKHTLAMVRSCGPDVPPHENPGVQLGLAMGLAGLKGRDKVTILSSRKIADFGAWAE
                     QLIAESTGKDGKGLIPIDGEPLGDVAIYGNDRFFIDIRTEGENDLAHDDRLAALEKAG
                     HPLVRIVMKSIEHIGQEFFRFELATAVAGAVLGINPFNQPDVEDAKIKTRELTAAYEK
                     SGALPAEQPVMSTALADLYTDDKNAAALRKAGADGDLGSWLKAHLARANAHDYVALLA
                     YIERDSAHIDALQQMRLEVRDMRHVATCAEFGPRFLHSTGQAYKGGPDSGVFLQITAD
                     DDKDLAVPGQKASFGVIKAAQARGDFDVLTERGRRALRVHLKGDLDAGLRMLDTAIQN
                     ALN"
     gene            2982..4019
                     /gene="gnd"
                     /locus_tag="VN968_08790"
     CDS             2982..4019
                     /gene="gnd"
                     /locus_tag="VN968_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HXL29372.1"
                     /translation="MQLGMIGLGRMGGNIVRRLMKHGHTTVVYDKDPKAVAALAADGA
                     AGSGALEDFVSKLEKPRTAWVMLPAGKITEATIDALSKLMQAGDVIIDGGNTFWQDDV
                     RRGKALKERGIHYVDVGTSGGVWGIERGYCMMIGGDKAVVDRLDPIFAGLAPGIGDIE
                     RTGGREGRDPRIEQGYIHAGPVGAGHFVKMVHNGIEYGLMQAYAEGFDILRNANIDAL
                     PAEHRFDLDIADIAEVWRRGSVIPSWLLDLTASALARNDTLDNYSGFVEDSGEGRWTV
                     NAAIDEAVPAEVLTAALFARFRSRKEHTFAEKILSAMRDGFGGHKEPQQHPDPARQTA
                     PGIIKPKADRS"
     gene            4016..5548
                     /gene="zwf"
                     /locus_tag="VN968_08795"
     CDS             4016..5548
                     /gene="zwf"
                     /locus_tag="VN968_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXL29373.1"
                     /translation="MTDVSSHQNQPASRKKPDPCSFVIFGVTGDLTHRLVVPSLYNLA
                     ATDLLPDKFCVVGIARKGTSNNALRDSLMKGLRQFATRPVDDEIAQRLLECVTSIEAD
                     PKDPASFDAVRERLEKLEANRSTGGNRLFYLATPPDAFAPISRELGRTGLLTEENGAW
                     RRLVIEKPFGTDLASAKALNGELLKIVDEHQIYRIDHYLGKETVQNILVLRFANGMFE
                     PIWNRNHIDHVQITVDEKLGVGHRGSFYDSTGALRDMVPNHLFQLLSLVAMEPPIRFD
                     AQSVRTEKADVLAAIQTQSEADALKNSVRGQYQGGKIGDTEIEDYCKTEDVKPGSTTE
                     TYSALKLTIDNWRWAGVPFYLRTGKALGIKRTEVAIKFKQAPFSMFSCTEVDRLSQNY
                     LIISIEPTEGITLQFNTKVPGPTINIDGVEMKFRYKDYFKAEPATGYETLIYDCMIGD
                     NILFQRADSVEAGWQAVQPFLDAWKKAGASGLQSYKAGSEGPADADALLARDGRSWRK
                     LG"
     gene            5553..>6252
                     /gene="pgl"
                     /locus_tag="VN968_08800"
     CDS             5553..>6252
                     /gene="pgl"
                     /locus_tag="VN968_08800"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HXL29374.1"
                     /translation="MAANEPHHVIAVSDAAALAKAAAERVMARIAANSGRVAICLTGG
                     SSPKQLYQLLATDAYRSRIPWERVHWFIGDERFVSADDPLHNMAVARRIFLDRSAPVS
                     NIHPIPTDTADPREAARHYESELKSFYGASELVSSRPLFDLVLMGVGPDGHTASLFPD
                     YPAIEEIERWVVGVPQAHVEPFVPRVTLTLPALGSCREMLFEVSGPDKRAILTRVLDG
                     ENLPANRARSTGETI"
BASE COUNT         1259 a         1962 c         1961 g         1070 t
ORIGIN      
        1 ctgatcgata ccgacggcgt caagggcgtc acgtcgaacc cgtcgatctt cgaaaaggcg
       61 atcggcagtt cggacgaata tgacggcgcc atcggcaagg ccctgaagaa gggcgatcgc
      121 ccgattgccg aactgttcga gcatctcgcc gtcgaggaca tccagcatgc cgccgacgtg
      181 ttgcgaccgg tttatgatga actgaagggc gaggacggtt tcgtcagttt agaggtgtcg
      241 ccctatctag cgatggatac caaagccacc atcgccgagg ccgaacggct ctggaaggac
      301 gtcaagcgca agaatttgat ggtcaaggtg cccgcgacgc cggagggcct gcccgcgatc
      361 cagcatttga tcggcgaagg catcagcatc aacattacgt tattgttctc gcaaaaagtc
      421 tatgtgcagg tcgcccaggc ctatctcgcc ggtctcgaga aatatgtcgc gaaaggcggc
      481 gatccctccc gtgtcgccag cgtcgccagt ttcttcgtca gccgcatcga cagcgcggtc
      541 gacaaggcgc tcgatgaaaa gatcgcacgg gccaacgatc ctaccgagaa ggagcggctg
      601 gccgcgctga aaggcaagat cgcgatcgcc aatgccaagc tcgcctatcg ggattacaag
      661 cgcctgttct ccggatcgcg ttgggacaag cttgcggcca aaggcgccaa gccgcagcgc
      721 ttgctgtggg cctcgaccgg taccaagaac aaggattaca gcgacgtgct ctatgtcgag
      781 gagctgatcg gccccaacac cgtcaacacc gtgccgccgg cgacgctcga cgcgttccgc
      841 gaccacggca cgccgcgcga cagtcttgag gaaaacatcg aggatgcgag gggcgtgctc
      901 gcggagcttg acacatccgg catatcgctc gatgcgatca cggccgatct ggtcaaggac
      961 ggcgtcaaac tgtttgccga tgccgccgac aaactttacg gcgcggtcgc gcacaagcgc
     1021 gcggcggtgc tcggggctgg tatcgaccgc caggagttcg cgctcggaag cagcatcggc
     1081 aaggcggtgg aaaagagcac cgaggactgg cgcgcgtcgg ccaaaatccg tcggctctgg
     1141 cagcacgatc aatcggtctg gaccgggact gatgaaaaca agtggctggg ctggctgaac
     1201 agcgccgcag cggccgatat cgccgactac gaggactttg cgcaccgtgt gaaaggacag
     1261 aacttcaccg acgcggtcgt gctcggcatg ggcggatcga gcttggggcc ggaagtgctg
     1321 gctcagacgt tcgaaaaaca gcccggcttc ccgaagctgc atgtgcttga ttcaaccgat
     1381 cccgcgcagg tccgcacgct tcaggcctcg atcaacatcg ccaatacgct gttcatcgtt
     1441 tccagcaaat ccggcggcac caccgagccg aacgtgatga aggattattt cttcgcccgc
     1501 gtctcggaga cgatcggcgc cgacaaggcc gggcatcggt tcatcgcggt gaccgatccc
     1561 ggctcgtcac tggagaaggt cgcaaccaga cagggttttg cgcgcatctt ccacggtgac
     1621 cccagcatcg gcgggcgcta ttcggtgctg tcgccgttcg ggctggtgcc ggcggcaacg
     1681 gcgggcatcg acgttgccac gctgctgaag catacgctgg cgatggtgcg ctcatgcggg
     1741 ccggatgtgc cgccccacga aaatccgggc gtgcagctcg gcctcgccat gggccttgcg
     1801 ggtctcaaag gccgtgacaa ggtcacgata ctctcgtcaa gaaagatcgc cgacttcggc
     1861 gcctgggccg agcagttgat cgcggaatcc accggcaagg acggcaaggg cttgatcccg
     1921 atcgacggcg aacccttggg cgacgttgcg atttacggca acgaccgctt cttcatcgac
     1981 atcagaaccg agggtgagaa cgacctggcg catgacgaca gactggcggc gctggaaaag
     2041 gcaggccacc ccctggtccg tattgtcatg aaatcgatcg agcatatcgg tcaggaattc
     2101 tttcgatttg agctggcgac cgcggtcgca ggcgccgtgc tgggcatcaa tccgttcaac
     2161 cagcccgacg tcgaggacgc caagatcaag acccgggaat tgaccgcggc gtacgaaaaa
     2221 tccggcgcac tgcccgccga acagcccgtg atgtccactg ccctggccga tctctatacc
     2281 gacgacaaga atgccgcggc cttgcgcaag gctggcgccg acggcgatct cggctcctgg
     2341 ctgaaggccc atctcgcccg cgccaatgcg cacgattacg tggcgctgct cgcctatatc
     2401 gaacgcgatt ccgcgcacat cgacgccttg cagcagatgc ggcttgaggt gcgcgacatg
     2461 cgtcatgtcg caacctgcgc cgaattcgga ccgcgtttcc tgcattccac cgggcaggcc
     2521 tacaagggcg gccccgacag cggcgtgttt ctgcagatca ccgccgatga cgacaaggat
     2581 ctcgcggtgc ctggtcaaaa ggcgagtttt ggcgtgatca aggctgccca ggcccgcggc
     2641 gatttcgatg tactgaccga gcgcggccgc cgcgctttgc gcgtgcatct caagggcgac
     2701 ctcgatgccg gactaaggat gctcgatacc gcgattcaga atgcgttgaa ctagagtcat
     2761 gagaatgcga gcggaaaccg ggcgttcggt cacctctccc acagggagag gtcggatcgc
     2821 gtcggcgatg caaggcatca tcgacacgat ccgggtgagg ggttacgctc tcccgttaga
     2881 ccgtcctccc tcttcccatg ggagagggag cttacctcca ctgccgcgac actgaggcga
     2941 aacatcaaga gcgacccgaa ccgaatttca aggaaagcaa aatgcagctc ggcatgatcg
     3001 gcttgggtcg gatgggcggc aatatcgtcc gccggctgat gaagcacgga cacaccaccg
     3061 tggtgtatga caaggatccg aaggccgtgg cagcgctcgc ggccgacggt gctgccggct
     3121 cgggcgcgct ggaagatttc gtcagcaagc ttgagaagcc gcgaaccgcc tgggtgatgc
     3181 tgccggcggg caagatcacc gaggcgacca tcgacgcgct gtcgaaactg atgcaggccg
     3241 gcgacgtcat catcgacggc ggcaacacgt tctggcagga cgacgtccgc cgcggcaagg
     3301 cgctgaagga gcgcggcatc cattacgtcg acgtcggcac gtcgggcggc gtgtggggca
     3361 tcgagcgcgg ctattgcatg atgatcggcg gcgacaaggc ggtggtcgat cgcctcgatc
     3421 cgatcttcgc aggcttggcg ccgggcatcg gcgatatcga gcgcaccggc ggccgcgagg
     3481 gacgcgatcc gcgcatcgag caaggctata ttcatgccgg cccggtcggc gccgggcact
     3541 tcgtcaagat ggttcacaac ggcatcgaat atggcctgat gcaggcctat gccgagggat
     3601 tcgacattct caggaatgcc aatatcgacg cgctgcccgc cgagcatcgc ttcgatctcg
     3661 acattgccga tatcgccgaa gtctggcggc gcggcagcgt gatcccgtca tggctgctcg
     3721 atctcaccgc ttccgcgctg gcaaggaacg atacgctgga taattactcc ggtttcgtcg
     3781 aggattccgg cgaaggccgc tggaccgtca acgccgccat cgacgaggcc gtgccggccg
     3841 aggtgctcac ggctgcgctg ttcgcacgct tccgctcgcg caaggaacat accttcgccg
     3901 aaaagattct ctcggcaatg cgcgatggtt ttggcggcca taaagaaccg cagcagcacc
     3961 ccgatccggc gcggcagacc gcgccgggga tcatcaagcc aaaggccgac cgctcgtgac
     4021 cgacgtctcc tcacatcaga atcagcctgc aagccggaag aagcccgatc cctgctcctt
     4081 cgtcatcttc ggcgtcaccg gcgacctgac ccaccggctg gtcgtcccct ccctttataa
     4141 tcttgcggcg accgacctct tgcccgacaa attctgcgtg gtcggcatcg cccgcaaggg
     4201 cacgtcaaac aacgccctgc gcgacagcct gatgaagggc ctgcgccaat tcgccacccg
     4261 gccggtcgac gatgagatcg cccaacgcct gctggaatgc gtcaccagca ttgaagccga
     4321 cccaaaggac ccggcctcgt tcgatgccgt gcgggagcga ctcgagaaac tggaagcaaa
     4381 ccggtccacc ggcggcaacc gcctgtttta tctggcgacg ccgcccgatg ctttcgcgcc
     4441 gatcagccgg gaactcggcc gcaccggcct gctcacggaa gaaaacggcg cgtggcggcg
     4501 gctggtgatc gaaaaaccgt tcggaaccga ccttgcctcg gcgaaagcgc tgaacggcga
     4561 attgctcaaa attgtcgacg agcaccagat ctatcggatc gatcactacc tcggcaagga
     4621 gacggtgcag aacatcctgg tgctgcgctt tgccaacggc atgtttgagc cgatctggaa
     4681 ccgcaatcac atcgaccacg tgcagatcac cgtcgacgag aagctcggcg tcggccaccg
     4741 cggcagcttt tacgactcaa ccggcgcgct ccgcgacatg gtgccgaacc acctgtttca
     4801 actgctgtcg ctggtcgcga tggagccgcc gatccggttc gacgcgcaat cggtgcgcac
     4861 cgaaaaggcc gacgtgctgg cggcgatcca gacccagagc gaagccgacg ctttgaagaa
     4921 ttccgtgcgc ggccagtatc aaggcggcaa gatcggcgac accgagatcg aggattattg
     4981 caagaccgag gacgtcaagc ccggcagcac caccgaaacc tattccgcgc tgaaactgac
     5041 catcgacaat tggcgctggg ccggggttcc cttttatctg cgaaccggca aggcgctcgg
     5101 catcaagcgc accgaagtgg cgatcaagtt caagcaggcg ccgttttcga tgttcagttg
     5161 caccgaggta gaccgcctgt cgcagaacta cctcatcatc agcatcgagc cgaccgaggg
     5221 cattacgctg cagttcaaca ccaaggtgcc gggaccgacg atcaacatcg acggcgtcga
     5281 gatgaaattc cgctacaagg actacttcaa ggccgagccg gccaccggct acgagacgct
     5341 gatctatgac tgcatgatcg gcgacaacat cctgtttcag cgcgccgaca gcgtcgaagc
     5401 gggctggcag gcggtgcaac cgttcctcga tgcgtggaag aaggcgggcg ccagcggcct
     5461 gcaaagttac aaggccggca gcgaaggtcc ggcggacgcc gacgcgctgc tggcgcgcga
     5521 cggccgcagt tggcgcaagc tcggttagcc ggatggccgc gaacgagcct caccatgtga
     5581 tcgcggtgtc cgatgccgcg gcgcttgcca aagccgccgc ggaacgggtc atggcaagga
     5641 ttgccgccaa tagcgggcgc gtggcgattt gcctgaccgg tggatcgagc ccgaaacagc
     5701 tctatcaatt gctcgcgacc gacgcctatc gaagccggat cccgtgggag cgcgtgcact
     5761 ggttcatcgg cgatgagcgt tttgtctccg cggacgatcc gctccataac atggcggtgg
     5821 cgcggcggat ctttctggat cgctccgcgc cggtttccaa catccacccg attccgaccg
     5881 acaccgccga cccccgggag gccgcgcggc attacgaaag cgagttgaaa tcgttctacg
     5941 gcgcgagcga gctggtttct tcccggccat tgttcgatct cgtgctgatg ggggtcgggc
     6001 ccgacggcca taccgcatcg ctgtttccgg attatcccgc catcgaggag atcgagcgct
     6061 gggtggtcgg cgtaccccaa gcccatgtcg agccgttcgt gccgcgggtg acgctgacct
     6121 tgccggcact cggctcctgc cgcgaaatgc tgttcgaggt gtccggcccc gacaagcgtg
     6181 cgatcttgac gcgtgtgctg gatggcgaga acctgcccgc caatcgtgcg cgatccaccg
     6241 gcgagacgat tt
//
LOCUS       DATNOI010000232         5066 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1099693,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000232 DATNOI010000000
VERSION     DATNOI010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099693"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1685
                     /locus_tag="VN968_08805"
     CDS             <1..1685
                     /locus_tag="VN968_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXL29375.1"
                     /translation="FLNLFIGLSITTAVGLWMVGSLNLLSIAFAVLFVGLGVDFGIQF
                     SVRYRSERFKGDDLRLALEKAAERSAVPLSLAAMATAAGFLSFLPTDYKGISELGKIA
                     GVGMLVAFTSSITVLPALLRLLNPPGESEPVGYAFLAPVDYFLEKHRVVIIVGTLLIA
                     VAGLPLLYFLRFDFNPINLRSPKVESIATFLDLRSDPNTGANAINVMTNSDADAKKIE
                     ERLSKVPEVLRAMSLDSFVPEDQPAKLKLIGQGARILGPALNPDSIDAPPSDQENVES
                     LKGSAENLRRTAGDAKGPGAVASRRLADALSKLADSDQATRDKTQAIFVSPLKVVLDQ
                     LKNSLQAQPVSLKTLPADLVNSWKTKDGLTRVEALPRGDPNDNDTLRKFAAAVLNAEP
                     NAIGGPVSILKSGDTVVKAFIHAGITALLVISFLLWLALRRVTDVLLTLVPLLVAGAV
                     TLEICVLIGLPLNFANIVALPLLLGVGVAFKIYYVTAWRSGRTNLLQSSLTRAIFFSA
                     LTTATAFGSLWLSSHPGTASMGKLLALSLVTTLAAVLLFQPALMGKPRDIGE"
     gene            complement(1653..2303)
                     /locus_tag="VN968_08810"
     CDS             complement(1653..2303)
                     /locus_tag="VN968_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HXL29376.1"
                     /translation="MRTVAYSGFFLATCFASSAFAADPTGDWKVADGVANIRVAECNG
                     GMWGAVSWEKVPGGHDINNPDVSKQSRPTLGMPILIGMKKKPGVDQWEGQVYNGKDGK
                     LYSSTIKPVGSDQLEIQGCVLGFLCGGETWTRVAGPIPSSPANSMAKGGPKTAGAALP
                     KAAAPTTKPAAPTTTATAPKTNPAAKTPGQKLATTGQPADAVGDICLLPDIAGFAH"
     gene            2443..3834
                     /gene="hpnO"
                     /locus_tag="VN968_08815"
     CDS             2443..3834
                     /gene="hpnO"
                     /locus_tag="VN968_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobacteriohopanetriol synthase HpnO"
                     /protein_id="HXL29377.1"
                     /translation="MLYPNLDVSEMFVDRQSQRSSMHARHLNEQLVRVLKTIGYDVGF
                     QKGQGQYLFDRDGARYLDLLSGFGVFAIGRNHPALRQALKSVLDSDLPNLVQLDVSTL
                     AGVLAERLLEFVPYLDKVFFANSGAECVEAAIKFARGATGRPGIVYCAHGYHGLTYGA
                     LSLTDDPNFRGGFEPLLPGCTSIPFNDLAALEQALSSRQVAAFIVEPIQGKGVNMPTD
                     EFLPGAAALCRKYGTLMVADEIQTGMGRTGRFLAVEHWNVEPDIVLLSKALSGGHVPV
                     GALLTRKSVFEKIFNQMDRAVVHGSTFAKNDLAMAAGIATLEVIESEKLVDAAAKRGA
                     ELRLALTRMVPGYELMKEVRGKGLMIGVEFGPPKSMRLKASWNLLETANKGLFCQLIT
                     VPLFKDHKILTQVSGHGSHTIKLLPSLTITEEDCSWIETAFDSVIASSHKVPGAIWSL
                     GKTLVDNAVRKSA"
     gene            3935..4264
                     /locus_tag="VN968_08820"
     CDS             3935..4264
                     /locus_tag="VN968_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXL29378.1"
                     /translation="MLEIRYYVAASGEGPFAEWFAELDPAASAKIVRALARIEQGNLS
                     NVKSVGEGVLEYRVDFGPGYRIYFGRDGETIIILLTGRTKKRQQRDIDAAHAYWLEYK
                     RSKRGRG"
     gene            4278..4589
                     /locus_tag="VN968_08825"
     CDS             4278..4589
                     /locus_tag="VN968_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXL29379.1"
                     /translation="MARTKSFKELVQGRVKADKKFAEALLREGIDAMLSGDVDTGKAI
                     LRDYIKATVGFEELGEATGAPPKSLIRMFGPRGNPQAKNLFSVIGYLQKRAGLSLHVA
                     G"
     gene            complement(4737..>5066)
                     /locus_tag="VN968_08830"
     CDS             complement(4737..>5066)
                     /locus_tag="VN968_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HXL29380.1"
                     /translation="FNGKLDVAPPVIDLTAQGFTLIGGRLDYVDTRPIGAIVYRRRAH
                     VINLFVAQTASAEPRSARIDSIQGFNIRRWSVRGLNYWAVSDLAADELAEFGDKFETA
                     MRTGTVG"
BASE COUNT          952 a         1520 c         1544 g         1050 t
ORIGIN      
        1 tgttcctgaa cctctttatc ggcctttcca tcacgaccgc cgttgggctg tggatggtgg
       61 gttcgctgaa cctgctctcg atcgcattcg ccgttctgtt cgtcggactg ggcgtcgact
      121 tcggcatcca gttcagcgtc cgctaccggt cggagcgatt caaaggcgac gacctgcggc
      181 tggcgctgga aaaggccgcc gaacgctccg cggtcccgct ttcactcgcc gcgatggcga
      241 cggcggccgg attcctctcg ttcctgccaa ccgactacaa gggcatttcg gaactcggca
      301 agatcgcagg cgttgggatg ctggttgcct ttacgtccag catcacggtg ctgccggctt
      361 tgctgaggct gctcaatcct cccggggaat cggagccggt cggttatgcg ttcctggcgc
      421 cggtcgatta tttcctcgaa aagcaccgcg tcgtcatcat cgtcggcacg cttttgatcg
      481 cggtcgccgg tctgccgctg ctttatttcc tgcggtttga tttcaacccg atcaacctgc
      541 gaagtcccaa ggtcgaatcg atcgcgactt ttctcgatct gcggagcgac ccgaacaccg
      601 gcgccaacgc catcaatgtg atgacgaact cggacgccga cgccaagaag atcgaagaac
      661 gcctgtccaa ggtgccggaa gtgctccgcg cgatgtcgct ggacagtttt gtgccggaag
      721 accagccggc aaaattgaag ctgatcgggc aaggcgccag gattcttggt cccgcgctca
      781 acccggactc gatcgatgcc ccgccgtccg atcaggagaa tgtcgagtcc ttgaagggat
      841 cggcggaaaa cctgcgcagg accgccggcg atgccaaggg ccccggcgcc gtggcatcgc
      901 ggcggcttgc cgacgcgctg tcgaagctcg ccgattcgga tcaggccacg cgggacaaga
      961 cccaggccat cttcgtttcg ccattgaagg tggtgctgga ccagctcaag aactcgctgc
     1021 aggcccagcc ggtcagcctg aagacactgc cggccgatct ggtgaattcg tggaaaacca
     1081 aggatggcct gacccgcgtt gaggcgctgc cacgcggcga tcccaacgac aacgacacac
     1141 tgcgcaagtt cgcagccgcg gttctgaacg ccgagccgaa cgcgatcggc gggccggtat
     1201 cgattctcaa atccggcgac accgtcgtca aggccttcat ccacgccggc attaccgcgc
     1261 tgctcgtgat cagctttctg ctttggctgg cattgcggcg agtaaccgac gtgctgctga
     1321 cactggtgcc gctgttggtc gccggcgcgg tgacgctgga aatctgcgtg ctgatcgggc
     1381 tgccgctcaa cttcgccaac atcgtggcgc tgccgttgct gctgggcgtc ggcgtcgcgt
     1441 tcaagatcta ttatgtcacc gcgtggcgct cgggcagaac caacctgctg cagtcgagcc
     1501 tgacgcgtgc gatatttttc agcgcgctga cgacagccac cgcgttcgga agcttgtggc
     1561 tatcgagtca tccgggtacc gccagcatgg gtaagctgct ggcgctctcc ctggtcacga
     1621 cgcttgccgc ggtgctgttg ttccagccgg cgctaatggg caaaccccgc gatatcgggg
     1681 agtaggcaga tatcgccaac cgcatcggcc ggctgccccg tcgtcgccag tttttgaccg
     1741 ggcgtcttgg cagccggatt ggtcttcggc gcagtggctg ttgttgtagg tgcggcgggc
     1801 tttgttgtcg gtgcggcggc cttgggtaga gcagcacccg cggtcttcgg cccgcccttg
     1861 gccatgctgt tggccgggct actgggaatc gggcccgcga cgcgggtcca ggtttcgccg
     1921 ccgcagagga agcccaacac gcaaccttga atttcaagtt gatcggaacc aaccggcttg
     1981 atggtcgaac tgtagagttt tccgtccttg ccgttataga cttggccttc ccattgatcg
     2041 acgccaggct tcttcttcat gccgatcagg atcggcattc ccagcgtcgg cctgctttgc
     2101 tttgagacat ccggattgtt tatgtcgtgg ccgcccggca ccttctccca cgacaccgcg
     2161 ccccacatgc cgccattgca ttctgcaacc cgaatgttgg cgacgccgtc cgccaccttc
     2221 cagtcgcctg tcggatccgc cgcaaaggct gatgatgcaa agcaagtcgc caaaaaaaat
     2281 ccggaatatg ctactgtacg catgatgttt gtacgcatga tgtttcgagg gcagtgcaac
     2341 atgatttaga cctgtgctta agtagaggat ccgagcgagg gcaaaacgcc gcattgagcg
     2401 ttttcagttg acggaatggc caccaaccga agtatgtaac tgatgctata tccaaatcta
     2461 gacgtttccg agatgtttgt ggatcgccag tcgcagcgca gttccatgca cgcgcgccac
     2521 ttaaatgagc aactggtgcg ggtcctcaag acaattggtt acgatgtcgg cttccagaaa
     2581 ggtcaaggcc aatacctgtt cgatcgcgac ggcgcgcgct atctcgatct tttgagcggt
     2641 tttggcgttt ttgcgattgg ccggaatcac ccggcgctgc gccaggcctt gaagagcgtc
     2701 ctcgacagcg acctgcctaa cctcgtgcaa ctcgatgttt cgacgcttgc gggcgtgctg
     2761 gccgaacggc tgctggaatt tgttccatat ctggacaagg tgttctttgc caattccggg
     2821 gcagaatgtg tcgaagctgc gatcaaattc gcccgcggcg ccaccggccg gccgggcatc
     2881 gtgtactgcg ctcatggtta ccacgggttg acctatggcg cgctgtcgct caccgacgat
     2941 ccgaactttc gcggcggctt cgagccgttg ctgcccggat gcacctcgat acccttcaac
     3001 gatctcgccg ctctggagca ggcgctgtcg tcgcgtcagg ttgccgcctt tatcgtcgag
     3061 ccgatccagg gcaagggcgt caacatgccc acggatgaat ttctgccggg cgccgcagcg
     3121 ctctgccgca agtacggcac cttgatggtg gccgacgaaa tccagaccgg aatgggtcga
     3181 accgggcggt tcctcgcggt cgagcactgg aatgtcgagc ctgacatcgt cctgctctcg
     3241 aaggcgctgt ccggcggtca tgttccggtc ggtgcgttgc ttacccgcaa gagcgtcttc
     3301 gaaaagatct tcaatcaaat ggatcgcgcg gttgtgcacg gatcgacatt cgcgaaaaac
     3361 gatctggcga tggcggccgg aattgcgacg ctcgaagtga tcgagtccga aaaattggtc
     3421 gatgcggccg ccaagcgcgg cgccgaactt cggctcgcgc tgacgcggat ggttcccggt
     3481 tatgaattga tgaaagaagt ccgcggcaag gggctgatga tcggcgtgga gttcggtccg
     3541 ccgaagtcaa tgcggctgaa ggcgtcgtgg aacctgctcg agaccgccaa caagggtttg
     3601 ttctgccagc tcatcacggt gccgctgttc aaggatcaca agatcctcac gcaagtctcc
     3661 ggccacggca gccacaccat caagctgctg ccgtcgctga ccattaccga agaagactgc
     3721 agctggatcg aaaccgcgtt cgattccgtc atcgcctcca gccacaaggt ccccggcgcg
     3781 atctggtcgc tcggcaagac actggtcgac aacgccgtgc gcaaatcggc ttaatcccgt
     3841 actccaaaga ctgtccttac gcagggcgcg atagcggagc ttattgccgt ctttgccctt
     3901 gaacgtaggg taacatatat gttaccctac gttcgtgctt gaaatccgct attacgttgc
     3961 cgcgagcggt gaaggtccct tcgccgaatg gttcgcggaa ctggacccgg cagccagcgc
     4021 caagatcgtc cgtgcgctcg cccgcatcga gcaaggcaat ctctccaatg taaaaagcgt
     4081 gggagaaggt gtgctggaat atcgggtcga ttttggtcct ggttatcgga tttatttcgg
     4141 acgggatggc gagacgatca tcatcctgct gaccggccga accaagaagc ggcagcagcg
     4201 cgatatcgat gcggcgcatg cttactggct ggaatacaag cggagcaagc gcggacgagg
     4261 ctgaacggag acgtgcgatg gcaagaacaa agagcttcaa ggaattggtg cagggtcggg
     4321 tcaaggccga caagaaattt gcagaggccc ttcttcgcga aggcatcgat gcgatgctga
     4381 gcggcgatgt cgataccggc aaggcgatcc tgcgcgatta catcaaggcg accgtcggtt
     4441 tcgaagaact gggcgaagca accggcgcgc cgccaaagag cctgatccgc atgttcggac
     4501 cgcgcggtaa tccgcaagcc aaaaacctct tcagcgtgat tggctacttg caaaagcgcg
     4561 cgggcctcag cctgcacgtc gccggctagg cgatcgcatc cggtctccgc agtcgtcggg
     4621 cgccaaatga gcaaattgcg gtgacagtcc attattgctt tgcggagccg gtcatcgggc
     4681 gcgcattcgt gcccgttggc gccgacccgt tggctggcaa tgacggtttg agaatcctag
     4741 cccaccgtcc cggtccgcat cgcggtttcg aacttgtcgc caaactcggc gagttcgtcg
     4801 gcggcgagat ctgagaccgc ccagtagttc aatccgcgca cgctccagcg cctgatattg
     4861 aatccctgta tcgagtcgat cctggcgcta cggggttcgg cgctggcggt ctgggccacg
     4921 aacagattga tcacatgcgc gcgccgtcgg tagacgatcg cgccgatcgg gcgggtgtcg
     4981 acgtaatcga gccggccgcc gatcagcgtg aagccctgcg cggtgaggtc gatcaccggc
     5041 ggcgccacgt ccagcttgcc gttgaa
//
LOCUS       DATNOI010000233        11845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1815396,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000233 DATNOI010000000
VERSION     DATNOI010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11845
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815396"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1481
                     /locus_tag="VN968_08835"
                     /pseudo
     CDS             1..1481
                     /locus_tag="VN968_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045207.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            1612..3552
                     /locus_tag="VN968_08840"
     CDS             1612..3552
                     /locus_tag="VN968_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXL29381.1"
                     /translation="MRRILLALAIALTVIALPGVTFAISDEETVRLLKRDADQGNANA
                     QGRLGYFYESGRGGLAKDDREAARLYRLAADQGNASARNNLGGLYRDGRGGLTKDDRE
                     AQRLFKLAADQGNAYGQLNLGLFNRDGRGGLSKDDREAARLFKLAADQAQADARVNLG
                     YFYETGHGGLAKDDREAVRLYRLAADQGNATAQSNLGVFYRDGRGGITKDDREALRLF
                     KLAADQGNPYGQNNLGGLYRDGGGGLAKDDREAGRLYKLAADQGNASARVNLGYFYEK
                     GRGDLAQDDREAARLYKLAADQGSAAGQNYLGVLYRDGRGGLTKDEREAARLFKLASD
                     QNYAYGQQNLGIFYRDGRGGLTMDNREAARLLKLAADQGNANARTALNQITQQTSQEV
                     TAAPNAPLKQEVVEPNLPTVPERRVALVIGNSAYQTVGGLRNTANDARAIAEVLKADG
                     FTSVRIILDMTRASMIASLNEFQREADTSDWAVVYYAGHGMEVSGANYLVPIDARLGD
                     DRDILDEAVSVDRILSAVENAKKLRLVMLDACRDNPFLKRMHRSIATRSISRGLAAIE
                     PMGATLIVFAAKDGETAEDGTGGHSPFAASLIKRLQQPTIEINKLFRLVTSDVLKATG
                     NQQRPFVYGSLPGEEDYYFRSR"
     gene            complement(3599..4198)
                     /locus_tag="VN968_08845"
     CDS             complement(3599..4198)
                     /locus_tag="VN968_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HXL29382.1"
                     /translation="MTRPSPFSVRGRRALMVMRDEREISIVRRQLSRLGMTIAEHDPA
                     EAPPDENADVVVMDADSIPIKSDLATAWKAGAPIIALIGTETPSRLKWLLDLQPASFL
                     IKPLHSAGLYTALVVAFDSAQRRIDETAHLERLEDRIRSRRVVFAAVLQIMRAHDLSE
                     SDAFRLIRQTAMRHRTTIEQLSAEIIAAGGMPNRTSWTA"
     gene            complement(4195..5376)
                     /locus_tag="VN968_08850"
     CDS             complement(4195..5376)
                     /locus_tag="VN968_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXL29383.1"
                     /translation="MKKPSVPLGLVFSRSGPYAMMAGEMGKSALMAIDEVNRSDEFDF
                     TFTPHLRDPGGVVAAYHTACDDLIREEQVDHIVGCYTSASRKQVIPIVERTERLLWHP
                     ARYEGFESSDNVIYVGAAPNHNVVPLVRHMLDQVSSDVFCIGSNYVWTWETNRVIREI
                     VTSAGGRILAERLLELGETAVDHIVREIIDRKPPAVFNTLVGESSYVFVRALHEAMIR
                     ARLTIPMLSCSLCEPELKLIGPAASVGYITSSAYFESIERPENRAFVARWKAGHGADS
                     SPSVDGQSTYVCVMLLARAIRRAGSTDVGAVRRAAANHRYDSPQGPVWVDPDNNHCFL
                     TPRLARSVPGCKFKIFWEADAPERPDPYLSNLDLTAVGTRLERSNDAMTKRSSHLRIV
                     K"
     gene            5738..7255
                     /locus_tag="VN968_08855"
     CDS             5738..7255
                     /locus_tag="VN968_08855"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXL29384.1"
                     /translation="MGVRLPTLEQINDLGSDFGLVLNADEVAAFQQAFKGPLASYGRL
                     DELVPPSLAPVAPRSPGYRPSASENPYGAWYWKTNIQTGAEGLLSGKKVAIKDNICVS
                     GVPMMNGSVLLEGYVPELDATVVTRILEAGGTIAGKAACEDLCFSGASHTCAGGVIRN
                     PHNPAHSAGGSSGGSAALVAAGEVPMALGGDQGGSIRTPSSWCGIYGLKPTWGLVPTT
                     GSMPISYSVDHCGPMGASVEDVARLLTVIAGHDGWDTRTISARTGDYMAALGKPAKGL
                     RVGILREGFGHPESDPAVDAKVRATIEALAKSGVEATEISLPWHLDGPHVWSGIILEG
                     AAEMMLKGYGVGNNIHGYYPLSMQEAFARGMGTRINDVSPTVKLVLMLGQYMHRNYHG
                     RYHSKAQNLRVLLRRAYDEAFEKFDVLAMPTIPFTATPIPPADAPLGTAIDVALNMQA
                     NTCSFDVSGHPAFTVPCGRVNGLPVGLMLVGRHFEETTLIRLASAIEAGGDWKLN"
     gene            7274..8677
                     /locus_tag="VN968_08860"
     CDS             7274..8677
                     /locus_tag="VN968_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXL29385.1"
                     /translation="MPTRDLHYCELVEIGQRIQRRELSPVEATQAQLDRIARLDGQLK
                     SYAYVMASSALEQAHAAEREIGSGKVRGPLHGVPIAVKDLCWTKDAPTAAGMRIYRDN
                     RPTQDATVVARLKEAGAVILGKLQLTEGAYADHHPDIDPPRNPWDATLWPGASSSGSG
                     VATAAGLCYGSLGSDTGGSIRFPSAANGVTGLKPTWGRVSRYGVFELAATLDHIGPMA
                     RSAVDCGAILGTIAGADPNDPTAVLEPVPDYLADLSGSLRGTRIGVDRRWTSEGVDAA
                     ATKVFQEALRVAAELGAEIHEIVFPDPTAMITDWFPLCGVETAVAHEATYPSRKPEYG
                     PGLAGLIDLGLQQSGKDYQKIVLRREAFKGMVRALFEKVDVLAVPAQTFAAPTLAKMA
                     ILGENPELITGLLRFSCPFDMTGSPTITLPGGFTESGGPVAFQFVGRHLDEARLVAAG
                     DAFQRVTDWHRRHPSLL"
     gene            8692..9690
                     /locus_tag="VN968_08865"
     CDS             8692..9690
                     /locus_tag="VN968_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HXL29386.1"
                     /translation="MTSASWLKTSIMAKRGLAKGEAGKRHELSIAAQGDFHYVYGPYA
                     RPVLTIDPGDVVVVETEDAFGGVITSEQDRPTAKLQFPFLNPQCGPVAVSGVEKGDCL
                     AVQIHAVETRGAQPAGTTCIIPEFGGLVGTASTAMLNAPLPERVRKMHVDKDGVRWSD
                     KIILPYEPFIGTIGVSPEIEAISSLQPDYHGGNMDLPDVAPGAILYFPVHTKGGLLYV
                     GDCHATQGDGELSGVAIEQRATVTLQVDVIKNWSFAWPRLETENFLMTIGSARPLEDA
                     ARIAYRELVRWMSADYGFDEIDAYMLLSQAGRIRLGNMVDPKYTMGASILKKYLVS"
     gene            9719..10942
                     /locus_tag="VN968_08870"
     CDS             9719..10942
                     /locus_tag="VN968_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL29387.1"
                     /translation="MKVNRRRFLQGTAAAAATGAFGSSAWAEDPINVASIHDLSGGLD
                     IYGKPMVDAITLAVEEANAAGGLLGRQIKLINYDTQSNMQLYTQFAQQAALKDKVAVV
                     HGGITSASREVIRPVLDRFKTLYFYNTQYEGGVCDRNQFDTGVTPAQTVEKLVPYAMK
                     KWGKKVYVIAADYNYGQITSQWVKKYVTENGGEVSSIDFFPLDVTNFGSTISKIQAAK
                     PDFVWSALVGGAHISFYRQWAAAGMRKSIPMASTTFAVGNEHIVLSPDECNDMLVCYN
                     YFQDLKNKTNETFVAAFQKRFGADYPNITELAMGTYQGFRLWTEAVKKAGSIDRMKVI
                     EALETGISIDAPSGKVTIDPPTHHCVLDVHIAEVKDKKLNVLEDFAQQKPSDTAAVCD
                     LIKNPADNQQYVIKI"
     gene            10982..>11845
                     /locus_tag="VN968_08875"
     CDS             10982..>11845
                     /locus_tag="VN968_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL29388.1"
                     /translation="MDLIALLAIQVVYAIASLALISVGLAIIFGMMRVINLAHGEFLM
                     LGGYAAIVATSHGISIWIAMLVVAPIVVGIIGVIVELTIIRFLYGRMVDTMLATWGLS
                     LFLVGLTTAIFGNTTVGISAPLGSFQIGAYRTSGYTLFVIAVAAVVMAGIFGVLRWTR
                     LGLIARGTMQNANMAAALGVNPPRVYAVTFGIGAALSGLAGAVLAPVSGVFPTIGVAY
                     VAKSFITVIGGGAAILSGTVSASALFGTINQIATFATTPVFGEVALLATAIVLIRLLP
                     QGITGRFFRRGL"
BASE COUNT         2372 a         3576 c         3667 g         2230 t
ORIGIN      
        1 ctccgatctg cgcatcatcg atcccggttt caccaccgcc tacatcacgc gcgatcacgg
       61 ctacatggtc tatgacacgc tgctggccac cgattccagt ttcaagatcc agccgcagat
      121 ggcggactgg aaggtctccg acgacaagct cacctacacc ttcacgctgc gcgacggcct
      181 gaaatggcac gacggcgcgc cggtcacggc ggaagattgc gtggcgtcgc tgaagcgctg
      241 gggcaggaac gacagcatgg gccagaagct gatggatttt accgccagca tcgaggccac
      301 cgacgccaag acgatcacgc tgaaactgaa agagccctac ggcctggtgc tggaatcgat
      361 cggaaaaccg tcgtcctata cgccattcat gatgcccaag cggttggccg aaacaccgcc
      421 cggtcagcag atcaaggagc agatcggctc cggccccttc aagttcgtgc aggcggaatt
      481 ccagcccggc gtaaaggcgg tgtatgagaa gaacgccgac tatgtgccgc gcaaggagcc
      541 gccgagctgg acctcgggcg gcaaggtggt gaaggtcgat cgcgtcgaat ggatcacgat
      601 ggcggacgcg cagaccgcgg tgaacgcgct gcagtccggc gacatcgatc tcatggagaa
      661 tccgccgttc gatcttctgc cggtgctcac cgccaacaag gacctgaagg tcgacatcct
      721 gaacaagctg ggcttccaga cgctcgggcg aatgaacttc ctttatccgc ccttcgacga
      781 cgtcaaggtt cgccgcgccg ccttgatggc catcaaccag aaggacgtgc tggatgcgct
      841 ggtcggcaat cccgaatatt acaagatctg tggcgcgttt ttcgtctgcg gcacgccgct
      901 cgccaccgag gaaggttcgg agtcgctggt caaaggcaat ggcatggcgg aagccaagaa
      961 gcagctcgcc gaatccggct atgacggcac cccggtcgtg atcatggcgc ccggcgatgt
     1021 cgtgacgctc aaggcgcagc cgatcgtatc ggcgcaattg ctgcgcgagg ccggcttcaa
     1081 ggtcgacgtg caggccaccg actggcagac cgtggtcgcc cgccgcgcca gccagaagcc
     1141 gccgaaggag ggcggctgga acatgttctt caccaactgg gtcggcgccg acgtggtcaa
     1201 ccccattgtc aacttctcga tcggcggccg cggcaagaat ggcggttggt tcggctgggc
     1261 cgaggacgcc aagatcgaac agatgaaaga cgccttcgcg cgctcgtcgt caccggagga
     1321 gcagaagaag atcgccgccg acatccagaa ggaagtctga ccaggttatc tacatcccgc
     1381 tcggccagta tcgagtggcc aatgcatggc ggaaatcgct caccggcgtg ctcgacggtc
     1441 cggccacgcc ggtcttctgg aacatcgata agtcggagtg aaaacgacgc cgcggggcag
     1501 cgcagcgatc gggcgtccgg ccggcgcgct ttgtcgcttg cgaggtttcg gccaaggaaa
     1561 ctaatatcgt aagcgcgtcc actccgccca cgcgatctga aggaatagct gatgcgtcgc
     1621 attctgctgg cacttgcaat agcgcttact gttattgcgc tgcccggcgt tacttttgcg
     1681 atcagcgacg aagagacggt acgccttctc aagcgcgacg ctgaccaggg taacgcgaat
     1741 gcgcaaggtc gtctcggcta tttctacgaa agcggtcgcg gtggccttgc caaagacgac
     1801 cgcgaggctg cacgcctcta caggctcgcc gccgaccagg gcaacgcctc cgcgcggaac
     1861 aatctcggcg ggctttacag agacggccgt ggcggcctca ccaaggacga ccgcgaggct
     1921 cagcgcctct tcaagctcgc cgccgatcag ggcaatgctt acggacagct aaatctcggc
     1981 ctcttcaacc gagatggccg cgggggtctt agcaaggacg accgcgaggc tgcgcggcta
     2041 ttcaagctag ccgccgacca ggcccaggcc gacgcgagag ttaatctcgg ctatttctac
     2101 gaaaccggtc acggtggtct tgccaaggac gaccgcgagg ctgtgcgcct ttaccggctc
     2161 gccgcggatc agggcaacgc cacagcacaa agcaatcttg gcgttttcta tcgagatggt
     2221 cgcggtggca tcaccaagga cgaccgcgag gctctgcgcc tcttcaaact cgccgccgat
     2281 caaggcaacc cctacggcca aaacaatctc ggcggtctct atcgagacgg cgggggtggc
     2341 cttgccaagg acgaccgcga ggctgggcgg ctctataagc tggccgccga ccagggcaac
     2401 gccagcgcga gagttaatct tggttacttc tacgagaaag gccgcggtga tcttgcccag
     2461 gacgaccgtg aggctgcgcg cctctacaag ctcgccgccg accagggcag cgccgccggg
     2521 caaaattatc tcggcgttct ttaccgggac ggtcgcggtg gtctcaccaa ggacgagcgc
     2581 gaagccgcgc gcctcttcaa gctggcctcc gatcagaact acgcctatgg gcaacaaaat
     2641 cttggtattt tctaccgaga cggtcgtggc ggtctcacca tggacaaccg tgaggctgcg
     2701 cgcctcctga aactcgccgc cgaccaaggc aacgccaacg cgcgaacggc cttgaaccag
     2761 atcacccagc agacctcgca agaggtaact gcggctccca atgcaccttt aaagcaagag
     2821 gtggtcgaac ccaatttgcc tactgtgccg gagcgccgcg tggcacttgt cattgggaat
     2881 tccgcttatc aaactgtagg cggactccgt aatacggcca acgacgcaag agcgattgcc
     2941 gaggtgctca aggccgacgg attcaccagc gtgcgaataa ttttggatat gacccgggca
     3001 agcatgattg cctccctcaa tgagtttcaa cgcgaagctg acacatcgga ttgggccgtc
     3061 gtctattatg ccggtcacgg gatggaggtt agtggcgcga attatctcgt gccgatcgac
     3121 gcaagactgg gggacgaccg ggatattctg gatgaggcgg tttcagtcga tcgaattctg
     3181 agtgcggtcg aaaacgcgaa aaagctcagg cttgtgatgc tcgacgcatg tcgcgataat
     3241 ccttttctga aaaggatgca tcggagcatc gcgacacgct cgataagccg cggactggct
     3301 gctatcgaac ccatgggcgc cacactgatc gtgtttgccg ccaaggatgg tgagacggcg
     3361 gaggacggaa ctggtggcca cagtccattt gcggcctcgc tcatcaagcg attgcagcag
     3421 ccaaccattg aaatcaacaa gcttttccgc ctcgtaacca gtgacgtcct gaaagctacc
     3481 ggcaatcagc agcggccatt cgtctatgga tcccttccag gcgaggaaga ctattatttc
     3541 aggtcgagat gaatttgcgc aggcgcgtgt tgggacgttg gagcgcgacg atccgaaatc
     3601 acgcggtcca cgatgtccgg ttcggcattc cgccggccgc gatgatttca gcactcaatt
     3661 gctcgatcgt cgtgcggtgc cgcatggccg tctgccggat cagcctgaac gcgtcggatt
     3721 cggaaagatc gtgagcgcgc atgatttgca gaaccgcggc gaacaccaca cgccgggagc
     3781 ggatgcggtc ctccagtctt tcgagatgcg cggtttcatc aattcttcgc tgcgccgaat
     3841 cgaacgcgac caccagcgcg gtgtagagtc cggccgagtg cagcggcttg atgagaaagg
     3901 atgccggctg caggtcaagc agccacttca accggctggg cgtctcggtt ccgatcagtg
     3961 cgatgatcgg cgcgccggcc ttccacgccg tggccagatc agacttgatg ggaatgctgt
     4021 ccgcatccat gacgacgacg tcagcgtttt cgtccggcgg cgcctcagcc ggatcatgct
     4081 cggcaatcgt catgccgagg cggctgagtt ggcggcgcac gatcgagatt tcacgctcgt
     4141 ctctcatcac catgagcgcg cggcggccgc ggacggagaa gggcgatggt cttgtcattt
     4201 cacgatacgc aggtggctgg accgttttgt catggcatca ttgctccttt caagcctcgt
     4261 gccaaccgct gtcagatcga ggttcgaaag ataggggtcg gggcgctccg gggcgtccgc
     4321 ttcccagaag atcttgaact tacacccggg cacggagcgt gccaaacggg gtgtcaggaa
     4381 gcagtgatta ttgtcgggat cgacccaaac cgggccttgc ggtgaatcgt accgatgatt
     4441 tgccgcagcg cgccggacgg ctccgacgtc ggtcgagccg gcgcggcgga tggcgcgggc
     4501 aagcagcatg acgcagacat aagtcgactg cccatcgacg gacgggctgc tgtcggcgcc
     4561 gtgaccggcc ttccaccgtg cgacaaaagc tcgattttcg ggccgctcga tgctctcgaa
     4621 ataagcagac gaggtgatgt atccgacgga ggccgccggt ccaatcagtt tgagctcggg
     4681 ctcgcacaga ctgcagctca gcatcgggat cgtcagtctc gcgcggatca tcgcctcgtg
     4741 gagcgcgcga acgaagacgt agctggattc cccgacaagc gtgttgaaca cagcgggcgg
     4801 cttgcggtcg atgatttccc tgacgatgtg gtcgacggcg gtctcgccga gctccagcag
     4861 gcgttcggca aggatgcggc cgcccgcgct tgtcacaatc tcacgaatga cccggttggt
     4921 ctcccaggtc cagacatagt tggacccgat acagaacacg tcgctggaga cttgatccag
     4981 catatgccgc accagcggga cgacgttgtg attgggagcg gcgccgacat aaatgacgtt
     5041 gtcgctgctc tcgaaacctt catatcgcgc cggatgccag agcagccgtt cggtgcgttc
     5101 gacaatggga atcacctgct tgcgtgacgc cgacgtatag cagccgacga tatgatcgac
     5161 ctgttcttcg cgaatgagat catcgcatgc cgtatgataa gcagcgacca cgccgccggg
     5221 atcacgcaga tgcggagtga atgtgaaatc gaactcgtcg ctgcgattga cttcgtcgat
     5281 ggccatcaac gcgctcttgc ccatctcgcc ggccatcatg gcgtaaggtc ccgatcggga
     5341 aaataccagg ccgagaggca ccgatggctt cttcataaag cagctcgaag gttgcgttgc
     5401 gaatgatcac atcgcaggaa tgtcgatctg tcaccttgta tttgtatcat caatctgcgc
     5461 tgcgcgtcct ttgtgctcgc ccatgcataa aaaaggtgcc ggcatcagat tgacaagccc
     5521 ggtgacgcta agacacaatc aattagtacg tgaatgcgtg catggcgaaa gagttctcgc
     5581 tcagccgatg acgtctgagc ggcaaccttc gattttcgtt ctgtaacgca ggactacgcg
     5641 accattgata gcagcggcgt ggatgccatt cggggcctgt ttgggtcgcg ggtggctttt
     5701 tttgtttgtc ttccaaaaga caagaattgg gtgaagcatg ggtgttcgcc ttccgacatt
     5761 ggagcagatc aacgacctcg gatccgattt cggtctggtt cttaacgctg acgaagttgc
     5821 ggcctttcag caggccttca aaggaccctt ggcctcctac ggccggctcg acgagttggt
     5881 cccgcccagc ttggcgccgg tggcgccacg ctcgcccgga taccggcctt cggcatccga
     5941 aaatccatac ggcgcctggt attggaagac gaatatccaa accggcgcgg aagggctgct
     6001 gagcggcaag aaggtagcga tcaaggataa tatctgcgtc tcaggtgtcc cgatgatgaa
     6061 cggctcggtg ctgctggaag ggtatgtgcc ggagctcgat gccacggtgg tgacgcggat
     6121 tcttgaagcg ggaggcacca ttgcgggcaa ggcggcttgc gaggatctct gtttttccgg
     6181 cgcaagccac acctgtgcgg gaggcgtgat ccgcaatccg cacaaccccg cgcatagcgc
     6241 tggcggctct tcgggtggaa gtgctgcgct ggtggcggcg ggtgaggtgc cgatggcgct
     6301 cggcggcgac cagggtggat cgatccgcac gccgtcaagc tggtgcggca tctatggcct
     6361 gaagccgacc tggggacttg tgcccaccac gggttcgatg ccgatcagct attccgtcga
     6421 tcactgcggt ccgatgggcg caagcgttga agacgtcgcc cggcttttga cggtcatcgc
     6481 cggccacgac ggctgggata cgcggacgat ttcagcgcgc accggcgact acatggccgc
     6541 gctcggcaag cccgccaaag gattgcgtgt aggaatcctg cgtgaaggat ttggtcatcc
     6601 tgaaagcgat ccggcggtgg acgccaaagt gcgcgcgacc atcgaagcat tggcaaagag
     6661 cggcgtcgaa gcaaccgaaa tctcgttgcc ctggcatctc gacgggccgc atgtctggag
     6721 cgggatcatc ctggaggggg cggcggagat gatgctgaag ggatatggcg tcggcaacaa
     6781 catccacggc tactatccgc tttcgatgca ggaagccttt gcccgcggca tgggaacgcg
     6841 gatcaacgac gtctcgccga ccgtgaaact ggtgctgatg ctcggccaat acatgcaccg
     6901 caactatcac gggcgttatc attcaaaagc gcaaaacctg cgggtgctgc tgcgtcgcgc
     6961 ctatgacgag gcgttcgaaa aattcgacgt gctggcgatg ccgacgattc cgttcaccgc
     7021 aacgcccata ccgccggcgg atgcgccgct cggcactgcg atcgacgtgg cgctcaacat
     7081 gcaggccaac acctgttcct tcgacgtctc ggggcatcct gccttcacgg tgccatgcgg
     7141 ccgcgtcaac ggattgccgg tcggcctgat gctggtcggc cgccatttcg aggaaacaac
     7201 cctgatacgg cttgcttcgg cgatcgaggc gggcggtgac tggaagctga actagaacac
     7261 gaggcaacac ttgatgccaa cgcgcgatct gcattactgc gaattggtcg aaatcgggca
     7321 acgtattcag agacgcgaac tgtctcccgt ggaggcaacg caagcccaac tcgatcgcat
     7381 cgcgcgtctc gacggccaat tgaaaagcta cgcctatgtc atggcttcgt cggcgctcga
     7441 gcaggcgcac gccgccgaga gggaaatcgg cagtggcaag gtgaggggac cgcttcatgg
     7501 ggtgccgatc gccgtcaagg acctgtgctg gaccaaggac gcaccgaccg cggcgggcat
     7561 gaggatctat cgcgacaacc gccccaccca ggatgcgacg gtcgttgcca ggctcaagga
     7621 ggccggcgcg gtcattctcg gcaagctgca actgaccgaa ggcgcttacg ccgaccatca
     7681 tccggacatc gatccgccgc gcaatccctg ggatgcaacg ctctggccgg gcgcatcttc
     7741 aagcgggtcg ggcgtcgcga ccgccgcggg gctttgttac ggctcgctcg gatccgatac
     7801 cggcggctcg atccgttttc cgtcagccgc caacggtgtg accggcctga agccgacctg
     7861 ggggcgggtc agccgctacg gcgtgttcga gctcgccgcg acgctggacc atatcggacc
     7921 gatggcacga agtgcggtgg attgcggcgc gatattgggc accatcgcag gcgccgatcc
     7981 aaacgatccg acagctgtgc tggagccggt accggactat ctggcggatc tgagcggcag
     8041 cttgcggggg acacggatcg gggtcgatcg gcgctggacc agcgaaggcg tcgatgccgc
     8101 tgcgaccaag gtttttcagg aggcgctgcg cgtcgcggcc gaactcggcg cggaaattca
     8161 tgaaatcgtc tttcccgatc cgacagccat gatcaccgac tggtttccgc tgtgcggcgt
     8221 cgagaccgcg gtcgcgcatg aggcgaccta tccctcgcgg aagccggaat acggtccggg
     8281 gctcgccgga ttgattgacc tcggtctgca gcaatccggc aaggactatc agaagatcgt
     8341 tctgcgccgc gaagcattca agggaatggt gagggcgctg ttcgaaaagg tcgatgttct
     8401 cgccgtcccg gcccaaacct ttgccgcgcc gacgctcgcc aaaatggcca tcttgggcga
     8461 aaatcccgaa ctgatcaccg gcctgttgcg cttcagctgt ccctttgaca tgacgggaag
     8521 tccgaccatt acgcttccgg gcggctttac cgagagtggc ggtccggtcg cgttccagtt
     8581 cgtcggccgc catctcgatg aagctcgttt agtagccgcc ggcgacgcgt tccagcgcgt
     8641 caccgactgg caccgccgcc atccttcact cctgtgattg aggagatgtc gatgaccagc
     8701 gcctcttggc tgaaaacttc gatcatggca aagcgcgggc ttgccaaagg cgaggccgga
     8761 aaacgccacg aactctcgat cgcggcgcaa ggggattttc attacgtcta tgggccctat
     8821 gccaggccgg tcctgacgat cgatcccggc gacgttgtcg tggtggagac cgaagacgcc
     8881 ttcggcggcg tcatcaccag cgagcaggac aggccgaccg cgaaactgca gtttcccttt
     8941 ctcaatccgc agtgcggccc ggtggccgtc agcggcgtcg agaagggcga ttgcctggcc
     9001 gtccagatcc atgccgtgga gacgcgaggc gcgcagcccg ccggcaccac ctgcatcatc
     9061 cccgaattcg gcggcctcgt cggaactgcg tcaacggcca tgctcaatgc gccgttgccg
     9121 gagcgggtgc ggaaaatgca tgtcgacaag gatggcgtgc gctggagcga caagatcatc
     9181 cttccctacg agccgtttat cggcaccatc ggggtttcac ccgagatcga ggcaatctcc
     9241 tcactgcaac cggactacca cggcggcaac atggatttac cggacgtagc gcctggcgcg
     9301 atcctttact ttccggtgca caccaagggc ggcctgctct atgtgggcga ttgccatgcc
     9361 acccagggcg atggcgaatt gtcgggggtt gcgattgaac agcgcgccac cgtcaccttg
     9421 caggtcgatg tcatcaagaa ctggagcttt gcgtggccgc ggcttgagac cgaaaatttt
     9481 ctcatgacca tcggcagcgc ccggccgctg gaagacgcgg cccgcatcgc ctatcgcgaa
     9541 ctggtgcgct ggatgagcgc ggactacggc ttcgacgaga tcgacgccta catgctcctc
     9601 agtcaggccg gtcgcatccg gctcggaaac atggtcgatc caaaatacac gatgggcgcg
     9661 tcgatcttga aaaaatatct ggtctcatga cgtatcggca acaacgagga gtacggcaat
     9721 gaaagtcaat cgcagaaggt ttttgcaggg cacggccgcg gccgccgcca ccggcgcgtt
     9781 cggttccagc gcctgggcgg aagacccgat caacgtggcc agcatccacg atctctcggg
     9841 tggtcttgac atctacggca agccgatggt cgacgcgatt acgctggcgg tcgaggaagc
     9901 caatgcggcc ggcggcctgc tcggacggca gatcaagctg atcaactacg acacgcaatc
     9961 gaacatgcag ctctataccc agttcgcgca gcaggcggcg ctgaaggata aggttgcggt
    10021 cgtgcatggc ggcatcacct ccgcctcgcg cgaggtgatc cggccggtgc tcgatcgctt
    10081 caagacgctg tacttctaca acacgcaata tgaaggcggc gtctgcgacc gcaaccagtt
    10141 cgataccggc gttacgcctg cgcaaaccgt tgaaaagctc gtgccttacg ccatgaagaa
    10201 gtggggcaag aaggtctacg tcatcgccgc cgactacaat tacgggcaga tcacttcgca
    10261 atgggtgaag aaatacgtca ccgagaacgg cggcgaagta tcctcgatcg acttcttccc
    10321 gctcgacgtc accaatttcg gctcgaccat ctccaagatc caggcggcga agcctgattt
    10381 cgtctggtcg gcgctggttg gcggcgcgca tatctcgttc tatcggcaat gggccgcggc
    10441 cggcatgcgc aagagcattc cgatggcgtc gaccaccttc gccgtcggta acgagcatat
    10501 cgtgctgtcg cccgacgaat gcaacgacat gctggtctgc tacaattact tccaggacct
    10561 gaagaacaag accaacgaaa ccttcgtcgc cgctttccaa aaacgcttcg gcgccgatta
    10621 tcccaacatc accgagctcg ccatggggac ctatcagggg ttccggctgt ggacggaggc
    10681 ggtcaagaag gccggcagca tcgaccgcat gaaggtcatc gaggcgctgg aaaccggaat
    10741 cagcatcgat gcgccctcgg gcaaggtcac gatcgatcct ccgacccacc attgcgtcct
    10801 cgacgttcac attgccgaag tgaaggacaa gaaactcaac gtgctcgaag acttcgcgca
    10861 gcagaagccg tcggacaccg cagcggtgtg cgacctcatc aagaatccag ccgacaacca
    10921 gcaatacgtc atcaagatct aaaggccggc tggcctgcaa ctctgtttgg gattagcgaa
    10981 cgtggatctc attgctctcc tggcgatcca ggtcgtttac gcgatcgcca gcctggcgct
    11041 gatcagcgtc gggctggcga ttattttcgg catgatgcgg gtcatcaatc tcgcacatgg
    11101 ggaatttctg atgctcgggg gctatgcggc gatcgtcgct accagccatg gcatcagcat
    11161 ctggatcgcg atgctggtgg tggcgcccat cgtggtcggc atcatcggcg tcatcgtcga
    11221 attgacgatc atccgcttcc tgtacgggcg gatggtcgac accatgctcg cgacctgggg
    11281 cctcagcctg tttctggtgg ggctgacgac ggcgatcttc ggtaacacca cggtcggaat
    11341 ctcggcgccg ctcggcagtt tccagatcgg tgcctatcgc accagcggat acacgctgtt
    11401 cgtcatcgcg gtggcggccg tcgtgatggc cggcattttc ggcgtgctgc gctggacccg
    11461 ccttggcctg atcgcgcgcg gcacgatgca gaacgccaac atggcggcag cacttggggt
    11521 caatccgccg cgcgtctacg ccgttacctt tggcatcggc gccgcgctgt cgggcttggc
    11581 cggcgcggtg ctggcgccgg tctccggcgt ctttcccacc atcggcgtcg cctatgtcgc
    11641 caaatccttc atcacggtga tcggaggcgg cgccgccatt ctcagcggca cggtttctgc
    11701 atccgccctg ttcggcacca tcaaccagat cgccacattt gccaccacac ccgtgttcgg
    11761 cgaggtggcg ttgcttgcga ccgccatcgt cctgatccgt ctgctgccgc agggcatcac
    11821 cggtcgcttc ttccggagag gtctg
//
LOCUS       DATNOI010000234         2841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_218751,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000234 DATNOI010000000
VERSION     DATNOI010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218751"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1051)
                     /locus_tag="VN968_08880"
     CDS             complement(155..1051)
                     /locus_tag="VN968_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HXL29389.1"
                     /translation="MTTTITPHTSRATFAIGSELTARRVVDRLTESFLEGEAAIAAFE
                     RSDGRWDVTLHFAEAPDQTSVRELVGIAAGGDVAQAITFDTVEAKDWVKATLDELVPV
                     RAGRFIVHGQHDRSRISPNKLGIEIEAALAFGTGHHGTTRGCLLLLDDVLKARRPKRV
                     LDLGTGTGVLAIAAAKASRVKMLASDIDPLAVRVARDNARLNGTGDLVETIRATGFSA
                     PEFTRHGPFDLVLANILANPLRQMATQMARHLAPSALAILSGLLPHQAQSVIAAYRAR
                     RLVLKRHLQIEGWSSLLMQRVS"
     gene            complement(1547..>2841)
                     /locus_tag="VN968_08885"
     CDS             complement(1547..>2841)
                     /locus_tag="VN968_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009336931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HXL29390.1"
                     /translation="APRSTAGAISRGGSRTAINGNRTFSSNRNANQRSRAVHQALNSR
                     AVAGALHHRDALRNPGTRARIAATAATAGWHNGHGRGWWRHGHGGYGWVGPLFWPFAY
                     YDLYGYAMWGYDPSFWDYGYNDIYAGMFAPYGYDDLAGYLPPGIGGNYVGRRQASRAF
                     ASTNPEPDQLTQMCGDDSRDIAGLPIDQIQQAVQPNETQRAALDDLANASVKAAQGIK
                     AACPTQLSLTAPARLAAMQQRIEAMIAAVGTVQPPLENFYGLLNDEQKARLNALGQDQ
                     QRRKSAQNTGGGSLAQSCGATQAGVTDWPAAEIDAKLHPTDAQRASLTALQNASARAD
                     DMLKGSCQTDDALTPPARLAAAGKRLEVMLQAITTVRTALDDFYGKLTDEQKAQFEAI
                     GPGRAGLSRQSDAAPTHSRRRHHAGFGGIIRHFMAFGW"
BASE COUNT          468 a          912 c          927 g          534 t
ORIGIN      
        1 ccattgccaa gaaacagcgg ggcgtcatcg ttcagattct ggatcgtttc gtcgaagccc
       61 gcgtcaagcg gacgttgcgc ggagagaagg gcgtcatcgc ccgccgcgaa aagaaaccgc
      121 cggagtagac gactgcctca cccgctacgt tagctcaact cacgcgctgc attaagagac
      181 tgctccagcc ttcgatttgc aggtgccgct ttagaacaag tcggcgcgcc cgataggcgg
      241 caatcacgct ttgagcctga tgcggcagca gccccgatag aatggccagc gccgatggtg
      301 cgagatgcct tgccatctgg gtcgccattt gccgcagcgg attggcgaga atattcgcca
      361 gtaccagatc gaacggccca tgcctcgtga actccggggc tgaaaacccg gttgcccgga
      421 tcgtctcaac cagatcaccc gtaccattga gccgcgcatt gtcgcgcgcc acccgaaccg
      481 cgagcggatc gatatcgctt gccagcatct tcacccgcga agccttggcc gcggcgatgg
      541 cgagcacgcc ggtgcctgtt cccaggtcga gcacgcgttt tggccggcga gccttcagca
      601 catcatcgag cagcagcagg cagccgcgcg tggtgccgtg gtggccggtg ccgaacgcca
      661 gcgccgcctc gatctcgatg ccgagcttgt tgggcgagat gcgcgagcga tcatgttggc
      721 catgcacgat gaatcgcccg gcccggaccg ggacgagttc gtcgagcgtg gccttgaccc
      781 aatccttggc ctcaaccgta tcgaacgtga tggcctgcgc gacgtcaccg ccggcggcga
      841 taccgacgag ttcgcggacc gacgtttggt cgggcgcctc ggcaaaatgc aacgtgacgt
      901 cccagcgccc gtcggatcgc tcgaacgcgg cgatcgcggc ctcgccctcc aaaaaactct
      961 cggtcagccg atcgaccacg cgccgcgccg tcagttcgct gccgatggcg aaggtcgcgc
     1021 ggctggtgtg gggtgtgatc gtggtggtca tcaagggtcc ggggagttgc aggcggatga
     1081 cgtgtgcgct tcacttaccc ggtaatcggg tcgtgcaaca gactgagctt cccgcggtat
     1141 ctcaacacac cgtccgaacg tttagggacg tgtcaccctg ccgccggttt ccttctatcg
     1201 cggccttgtg cggcaatggg ttacggtccg aactgcaagc gctgttcgag cggcttgcag
     1261 tggaatccgc gtttgatact gccgtgcgtc cacagcctgc ttatgccgaa cgcaagggtt
     1321 gttcacagcg ttctgcaccg aaacataccc ggacaaaccc gcgtttttca caggcctgcg
     1381 ccggatatct gcaccggaaa tccacaggct tctccaccga gtcatccacc ggttttccac
     1441 aatttttcca ccggttgtcg accgactcgt cctcaggcaa atagccggcc gcgcggcgtc
     1501 gtggccgggc gggggtggcg ccggcggcgg gattagtatg atgcgcttac cacccgaagg
     1561 ccatgaaatg ccggatgatt ccgccgaagc cggcgtgatg gcgccgacgg gagtgcgtcg
     1621 gcgcggcatc tgactggcgg gacaggccgg cacggcccgg gccgatcgcc tcgaactgcg
     1681 ccttctgctc gtcggtcagt ttcccataga aatcatccag tgcggtgcgc accgtcgtga
     1741 tcgcctgcag catgacttcc agccgtttgc cggcggcagc gaggcgggcc ggcggcgtca
     1801 gcgcatcatc tgtctggcag gaacccttga gcatatcgtc cgccctggcg ctggcgttct
     1861 gaagggcggt gagacttgcg cgctgcgcgt cggtcggatg cagcttggcg tcgatctccg
     1921 cagccggcca gtccgtgacc ccggcctgtg tggcgccgca gctctgggcg agcgagccgc
     1981 cgccggtgtt ctgcgccgac ttcctgcgct gctggtcctg tcccagcgcg ttgagccggg
     2041 ccttctgctc gtcgttcagc aggccgtaaa agttctcgag tggcggctgt accgtgccga
     2101 ccgcggcaat catcgcttcg atgcgttgct gcattgccgc gagccgggcc ggtgccgtca
     2161 acgataactg ggtgggacag gcggctttga tgccttgggc cgccttcacc gaggcattgg
     2221 cgagatcgtc gagcgccgcg cgctgggttt cgttgggctg aacagcctgc tggatctgat
     2281 cgatcggcag gccggcgatg tcgcggctgt cgtcgccgca catttgcgtc agctgatctg
     2341 gctccggatt ggtgcttgcg aaggcacgcg atgcttgccg cctgccgaca taatttccgc
     2401 cgatgcctgg cggcaagtag cccgcgagat cgtcataccc gtaaggcgcg aacatcccgg
     2461 catagatgtc gttgtagccg taatcccaga acgaggggtc gtagccccac atcgcatagc
     2521 cgtacaggtc gtaataggcg aacggccaga acagcgggcc gacccagcca tagccgccat
     2581 ggccgtgtcg ccaccagccg cggccgtgcc cgttatgcca tccggcggtc gccgccgtcg
     2641 cagcgatccg ggcgcgggtg cctggattgc gtaacgcgtc ccggtgatgc aaagcgccgg
     2701 ccaccgcgcg cgaattcagc gcctggtgta cggcgcggga tcgctgattg gcgttgcggt
     2761 tggagctgaa tgtccggttg ccgttgatcg ctgtcctgct gccgccacgg gaaatcgccc
     2821 cagcggtgga tcggggagcc g
//
LOCUS       DATNOI010000235         5930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2001421,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000235 DATNOI010000000
VERSION     DATNOI010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001421"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1357)
                     /locus_tag="VN968_08890"
     CDS             complement(119..1357)
                     /locus_tag="VN968_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL29391.1"
                     /translation="MDFNMSDRQREWLNRVDSFMHKHVRPAVPTYRQQDEAGERWKVI
                     PILEDLKKKARAEGLWNMFMPPSAHEDDEFRGAGLTNLEYAPLAEQMGHIGWASEVFN
                     CSAPDTGNMEVLMRYGTKEHKKKWLRPLMDGEIRSAFLMTEPAVASSDASNIETSIKR
                     DGDHYVINGRKWWSSGVGDPRCQVLIVMGKTDTSAPRHQQQSQILVPRDAKGITVEKM
                     LPVFGFDDAPHGHAQVLLEDVRVPASNILLREGRGFEIAQGRLGPGRIHHCMRTIGKA
                     EEALEKMVKRLSSRTAFGRKIIEYSIWEQRIAEARTDIEMNRLLCLKAADMMDKVGNK
                     SAQLEIAMIKVAAPNMALKIIDNAIQAFGGAGVSDEAGLARDYASMRTMRLADGPDEV
                     HNRAIARLELRKYANSPTTH"
     gene            1587..2276
                     /locus_tag="VN968_08895"
     CDS             1587..2276
                     /locus_tag="VN968_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL29392.1"
                     /translation="MPSDHTRTAILSAAERLYADRGFADVTLRDIVAAADVNLAAVNY
                     HFGSKDELIAELFVTRSLATNRERLNELKAAEAAGGGRANIDSILRALVGPTLRGCLG
                     PDRERSAAARFMIRASIESVPPIRRIKNREIDHLRKFAAAMRRSLPDRSEVELYWGLH
                     FALAMAHQTIRDGERLTKLSEGLCDLNDVQGIIDRVVTVSVMALTGGEAQSKSPARPV
                     APYGRLTRQDL"
     gene            2281..2565
                     /locus_tag="VN968_08900"
                     /pseudo
     CDS             2281..2565
                     /locus_tag="VN968_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493192.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2750..3178)
                     /locus_tag="VN968_08905"
     CDS             complement(2750..3178)
                     /locus_tag="VN968_08905"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HXL29393.1"
                     /translation="MADENKTPIFGDVGKMPSFDFTKLMKDFKLPGVDLATIMDREKK
                     NIEALTEANRVAFEGWQALVHRQAEILQDSIKQAVGTAQGEDAGTKRMDLASHAFETA
                     LSNMRELAEMAAKSQKDAFEVIRKRVEENLAALRAPGTDE"
     gene            complement(3258..4139)
                     /locus_tag="VN968_08910"
     CDS             complement(3258..4139)
                     /locus_tag="VN968_08910"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXL29394.1"
                     /translation="MSGIRHGLDLDQSNAGVSGTSRDMGNRPANLRQVQADDQSSGYL
                     SRVPIIISGRTARRTLEACERELIQYKAMERRLRDALAEREARLRQRDELIRRQELLK
                     RESDHRLLNDLQMTISLLSLQSRGATNAEAASQLAVAANRVAMIARIHHRLNSYGGVQ
                     TIEFKKFLEDFGLDFSAMVSSHERPEQVVVEGIEINLPVATAIPLGFIASELITNAAK
                     YGKSRITIRLEPDPEYGYALSVSNDGPALPEGFDPAASAGLGMRIIQSFVRQIGGALR
                     SGRGDDGEGARFTVQFR"
     gene            complement(4224..4616)
                     /locus_tag="VN968_08915"
     CDS             complement(4224..4616)
                     /locus_tag="VN968_08915"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXL29395.1"
                     /translation="MKREAIRVEPLSSYLAKWQAPISPVTRAGNMVFVSGFPPFDPET
                     GEILSVPIERQTELILEQMKLCLETAGTSLQNVMKCNIYCTSAKHFPVVNAIYARYFP
                     EDPPARIFVCVPEWPGPFDIEIDCVAVI"
     gene            4692..5444
                     /locus_tag="VN968_08920"
     CDS             4692..5444
                     /locus_tag="VN968_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXL29396.1"
                     /translation="MEVWCGARAPRGRASAVIVKASLRYGSGPPARPWSQYRKARAPL
                     ADVLFPTIGVHNNALETDMPFLAKVGGLAILAGAGAAVFFFGGYYSVAGTAEDPAIVT
                     WALTRVRTASIDRHAVDQPPASINDPASVQAGAKAFAAHGCTTCHGGPGVTWAKFSEG
                     LHPDPPDLKEVVDHRSPAQLFWVVRNGINMTGMPSFALAGVKDDEIWSIVAFLKKLPG
                     VSEADYKAWTAPSSAPAAATPSAAPAPPAENK"
     gene            complement(5459..>5930)
                     /locus_tag="VN968_08925"
     CDS             complement(5459..>5930)
                     /locus_tag="VN968_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29397.1"
                     /translation="TMWLDDIEGSTETVFPDRPAFLALVALHDMLKPTPLFRVGSWLL
                     VCIIVCGVAWRRRETPEGAFALGVCGSAIVYVLTFVTVGVASDFRYGYWAVLAGIVGG
                     VVAALEHDPEKWVPVFRKDRAQTKDRADDDSKKSHPALGRPGPGVPAKARRKES"
BASE COUNT         1118 a         1868 c         1745 g         1199 t
ORIGIN      
        1 gatccgatgc cgctcctcga cgggcttggt gcccgaaaat tcttcgtctc ttctgacgcc
       61 gtcggccacg atgacgctcc ctcaagtttt tttaaggagc gccacgtgtg ggcccccgtt
      121 agtgcgtagt gggagagttt gcatacttcc gaagttcaag tctggcaatg gctcggttat
      181 ggacctcgtc cgggccgtcc gccaggcgca tcgtgcgcat cgaggcgtaa tcgcgcgcaa
      241 gaccggcttc gtcggagacc cccgccccac cgaacgcctg gatggcgttg tcgatgatct
      301 tcagcgccat attcggcgcc gccaccttga tcatggcgat ttcgagctgc gcggacttgt
      361 tgccgacctt gtccatcatg tcggcggcct tcaggcacag caggcgattc atctcgatat
      421 cggtgcgggc ttccgcaatg cgctgctccc agatcgaata ttcgatgatc ttcctgccga
      481 acgcggtgcg cgacgacagc cgcttcacca ttttctcaag tgcctcctcg gccttgccga
      541 tggtgcgcat gcagtgatgg atgcggccgg gacccagacg tccctgcgcg atctcaaagc
      601 ccctgccctc gcggagcaga atgttgctgg cgggaacgcg gacgtcttca agaagcacct
      661 gcgcgtggcc atgcggcgca tcgtcgaaac cgaacaccgg cagcatcttc tcgaccgtga
      721 tgcccttggc atcgcgcggc accaggatct gcgactgctg ctgatgacgc ggcgcgctgg
      781 tatcggtctt gcccatcacg atcagaacct gacagcgcgg atcgccgacg ccggacgacc
      841 accatttgcg gccgttgatc acgtaatgat cgccgtcgcg cttgatgctg gtttcgatat
      901 tggaggcatc cgacgacgcc accgcgggct cggtcatcag gaaggcggaa cggatttcgc
      961 cgtccatcag cgggcgaagc cacttcttct tgtgctcctt ggtgccgtac cgcatcagca
     1021 cttccatgtt gccggtatcg ggcgccgagc aattgaacac ttccgaagcc cagccgatat
     1081 ggcccatctg ctcggccagc ggcgcgtatt ccagattggt caatcccgcg ccacggaatt
     1141 catcgtcctc atgcgccgac ggcggcatga acatgttcca caggccttcg gcacgcgcct
     1201 tcttcttcag gtcttccagg atcgggatca ccttccagcg ctcgccggct tcgtcctgct
     1261 gccggtaggt cggcacggcg ggccgcacat gcttgtgcat gaacgaatcc acacggttga
     1321 gccattcgcg ctgacggtca gacatgttga agtccattgg gcgttcctca catttttggc
     1381 gaaaactttg gcggcactgt ctccccgcag cctgttcacc gcaagactat tatcgcgcgt
     1441 gtcagcgcgg cggtggcggc cggcgtggta tctgccggag tggcgatgcg tcatgcgcat
     1501 tgacattccg gccttcaaac catagtttca tacaactgtt tgaaatgcaa ctccgtcagt
     1561 ttgcacttgt cgcataggcc catgacatgc cgtcggatca caccagaacc gcgattctca
     1621 gcgccgccga acggctttat gccgatcgcg gcttcgccga cgtcacgctg cgcgacatcg
     1681 tggccgcagc ggacgtcaat ctcgcggcgg tgaactatca tttcggctcc aaggatgagc
     1741 tgatcgccga actgttcgtc acccgcagcc tcgccaccaa ccgcgagcgg ctcaacgaac
     1801 tgaaagccgc ggaagcagcc ggcggcggac gcgccaatat cgattccatt ctgcgcgcgc
     1861 tggtggggcc gacgctgcgc ggttgtctcg gccccgaccg cgagcgctcg gcggcggcgc
     1921 gtttcatgat ccgggcctcg atcgaatcgg tgccgccgat ccgccgcatc aagaatcgcg
     1981 aaatcgatca tttgcggaaa ttcgccgccg cgatgcggcg ctcactaccc gaccgcagcg
     2041 aggtcgagct ctattgggga ttgcatttcg cgctcgcgat ggcgcaccag accatccgtg
     2101 acggcgagcg attgacaaag ctgtcggaag gcttgtgcga tctcaacgac gtgcagggga
     2161 tcatcgaccg cgtggtgacg gtttcggtga tggcgttgac aggaggtgag gcgcaaagca
     2221 agtcgcccgc ccggccggtg gcgccgtatg ggcgactaac ccggcaagac ctgtgaaccc
     2281 tatctgttga accggaatag ccgaatcgaa gcggatctga tcgaccagtt gttcaattcc
     2341 agcgagaagc gtttggcccg cctgctgctg ctggcgaact ttggcaagga tagcagtccg
     2401 cagccgattg ggaatcacat caacccggaa acgctggcgg agatgatcgg caccacccgg
     2461 tcgcgcgtca gcttcttcac gaacaaattt cgcaagctgg gcttcatcag ctacaacggc
     2521 gagatcgagg tgcaccattc actgttgaac gcggttctgc acgacagcca gcagacccag
     2581 gggggactag cctgcgctcg ccgactcgaa accgcattcg gcgccgtctg gtcgagagtc
     2641 gcacgatcgg ttcgcagggc tacggcgagc gcggtagcag tcaactgcca cgcacgatcc
     2701 gggcattaaa atcaggctcg ccgccggcga tccgggtcgc gagcgtattc tattcgtctg
     2761 ttccgggggc acgaagggcc gccagatttt cctcgacgcg cttgcgaatg acctcgaacg
     2821 cgtccttctg agatttggcc gccatctcgg ccagctcgcg catattcgaa agcgcggttt
     2881 cgaaagcgtg cgacgccaag tccatccgct tggtgccggc atcctctcct tgcgccgtgc
     2941 cgacagcctg cttgatcgaa tcctgcagaa tctcggcctg tcgatggacc agcgcctgcc
     3001 agccttcgaa tgcgacacga ttggcctcgg tcagagcttc gatattcttc ttttcgcggt
     3061 ccatgatcgt cgcaagatcg actcccggca gcttgaaatc cttcatcagc tttgtgaagt
     3121 cgaagctcgg catttttcca acgtcgccaa atattggggt cttgttctcg tcagccattt
     3181 gcgtcgctcc tgtcgtgagg gttgctgtcc tgttcgataa ccatcgctgg ccgccatccg
     3241 gcgaggtccg cggccattca gcgaaactgc acagtaaatc gcgcgccctc gccatcgtcg
     3301 cctcgaccgg accgcaacgc gccgccaatc tgccggacga aagattgaat aatcctcatg
     3361 cccagtcccg cgctggcggc ggggtcgaac ccctccggca aagccgggcc atcattggaa
     3421 accgaaagcg cgtagccata ttccggatcc ggctccaggc ggatggtgat ccggctcttg
     3481 ccgtactttg ctgcgttcgt gatcagctcg ctggcaatga agccaagcgg aatcgcggtg
     3541 gcgaccggca gattgatttc aatgccctcg acgacgacct gctccgggcg ctcatgcgaa
     3601 gacaccatcg cggaaaaatc gagaccaaag tcctcaagga acttcttgaa ttctattgtt
     3661 tgcacacctc cgtaggaatt aagccgatgg tggattcgcg caatcatcgc cacgcgattg
     3721 gccgcaacgg ccaattgcga ggccgcttct gcattggttg ctccccggct ctgcaacgaa
     3781 agcaggctga tggtcatttg caggtcgttc aacaaccgat gatcggactc cctcttcaat
     3841 agctcctgtc gacggatcag ctcgtctctc tgacgaagcc gggcctcgcg ttccgccaag
     3901 gcgtcgcgca gacgtctttc catggctttg tattgaatca actcgcgctc gcaggcctcg
     3961 agcgtgcggc gcgccgtcct cccggagatg ataattggga cacgcgacaa atacccgctg
     4021 ctctggtcgt cggcctgaac ctgccgcagg tttgctggtc gattgcccat gtctctggat
     4081 gtgcccgaga cgcccgcatt ggattgatcc aaatcaagtc cgtgtcggat gccggacatt
     4141 atttggctgg gtaaatagcg gcagcgttcc cagtcgggaa caagcaggcc gccggaacga
     4201 caagcccgcg tcctccggcc cgatcagatc accgcaacgc aatcgatttc gatgtcgaac
     4261 ggccccggcc attccggcac gcagacgaag atgcgcgcgg gcggatcttc ggggaaatag
     4321 cgcgcgtaga tcgcattgac gaccgggaag tgcttggccg aggtgcagta gatgttgcac
     4381 ttcatcacat tctgcaggct tgtgcccgcg gtctccagac acagcttcat ctgctccagg
     4441 atcagttcgg tctggcgctc gatcggcacc gacagaatct cacctgtctc gggatcgaac
     4501 ggcgggaacc ccgagacaaa caccatgttg ccggcacgcg tgaccggcga gatcggcgcc
     4561 tgccatttcg caagatagga agacagcggt tcgacgcgga tggcttctcg tttcatcgca
     4621 atgctttcgg gtgcggcttg atctgacaac gaagaactag cacgggcagc gcggatcatg
     4681 cttcgagcgc gatggaagtg tggtgtggcg cgcgcgcccc ccgcgggagg gcgtccgctg
     4741 tcatcgtcaa agcctcgttg cggtatggtt ccggcccccc ggccagacca tggtcacaat
     4801 atcgtaaagc gcgcgcaccg ttggccgatg tattatttcc caccattggt gtgcacaaca
     4861 acgcgttgga gaccgacatg cctttccttg cgaaggttgg cgggctggca atccttgccg
     4921 gcgccggtgc agcggtcttc tttttcggag gctattatag tgtcgcaggg accgccgaag
     4981 acccggccat tgtcacctgg gcactgaccc gggtgcgcac ggcgtcgatc gatcgccacg
     5041 cagttgacca gcctccggct tctatcaacg atcctgccag cgtgcaggcc ggcgccaaag
     5101 cgtttgccgc acatggctgc accacttgtc atggcggacc gggcgtcaca tgggccaaat
     5161 tctcggaagg actgcatccc gatccgccgg atctgaagga agtcgtcgat catcgctccc
     5221 cggcgcagtt gttttgggtg gtcaggaacg gcatcaacat gactggcatg ccaagcttcg
     5281 cgctggcggg tgtcaaggac gatgagatct ggtcgatcgt cgcgttcctg aaaaaactgc
     5341 cgggcgtctc ggaagctgat tacaaggcat ggacggcacc atcgtccgcc cccgccgcgg
     5401 ccacgccttc tgcagcacct gcgccccctg cagagaacaa gtagattggc ttcgctgctc
     5461 aggattcctt ccggcgcgct ttcgccggga cgccgggccc gggccggcct agagcgggat
     5521 gacttttctt cgaatcgtca tccgctctat cttttgtttg agcacgatct tttcggaaaa
     5581 ccggtaccca cttttccgga tcatgctcta gcgcggcgac gacgccacca acgatgccgg
     5641 cgagaacagc ccaatagccg tagcgaaagt ccgacgcgac gccgaccgtg acgaaggtca
     5701 gcacatagac gatagccgat ccgcaaacgc cgagtgcgaa ggcgccctct ggcgtctcgc
     5761 gtcgtctcca ggcgaccccg catacgataa tgcaaaccag caaccacgag ccaacccgga
     5821 acagcggcgt cggcttgagc atgtcgtgca gcgcgaccag cgcgaggaag gccgggcgat
     5881 ccggaaatac ggtctcagtc gaaccctcga tgtcgtcgag ccacatcgtg
//
LOCUS       DATNOI010000236         2213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_744255,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000236 DATNOI010000000
VERSION     DATNOI010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744255"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VN968_08930"
     CDS             <1..454
                     /locus_tag="VN968_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL29398.1"
                     /translation="EGIVLQTADVKVTAFRTPHPPIMDNFAYKFETPDGVIVFSSDTA
                     YNPKLAEFAKGADVLVHEALYVPWVDRLVARVKNGATLKKHLLESHTAVEDVGKIAAA
                     AGVKVLVLSHLVPGDLDVTDDTWSEGAKKNFSGKIVVARDLMELKLPV"
     gene            complement(487..1893)
                     /locus_tag="VN968_08935"
     CDS             complement(487..1893)
                     /locus_tag="VN968_08935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL29399.1"
                     /translation="MTMTRRNFLSASAALAVTPALHTDAWGAPLPREADIVVIGAGAA
                     GIAAARRIQAANRRVIVVEATGQIGGRCLTDARTFDVPFDRGARWMHNPDTNPMIKLA
                     RNAGLETYPAPPGQKIRVGRRNARAGEIEYFLAALVRANRAIDDASRGKADIACASAL
                     PKDLGDWAATVEFTLGANAAGRDLKDISAIDKARGQDRNNAQIACRQGLGTLIAKLGE
                     QVPLALSMPASRIAWDRREVAVDTPAGKIAARAAVITVSSNVLTAGNIKFTPDLPKRS
                     LDAAAKLSLGSYDHIALQLPGNPLGLARDEIIIEQSTSQRTGLLFANIGASSLCSIDV
                     GGSFGRDLAAQGEPAMIAFAVEWLTKLFGSDAAAAVKKSSATRWDIAPFALGAMSVAA
                     PGAQPSRKILTEPIGCMFLAGEATHETLWGTVDGAWESGERAAEAALRKIGALKDTEP
                     ATQAAPRVKRRHRGASIN"
     gene            2097..>2213
                     /locus_tag="VN968_08940"
     CDS             2097..>2213
                     /locus_tag="VN968_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipo-like protein"
                     /protein_id="HXL29400.1"
                     /translation="MGVVLDTIGKLIADYLQKEIPGYEPFTPSDPEHLRGVIE"
BASE COUNT          402 a          705 c          703 g          403 t
ORIGIN      
        1 ggagggcatc gtgctgcaga cggctgatgt aaaggtgacg gcgttccgaa cgccgcatcc
       61 gccgatcatg gacaatttcg cctacaaatt cgagacgcct gacggcgtca tcgtgttctc
      121 cagcgacacc gcctacaatc cgaagctcgc ggagttcgca aaaggtgccg acgtgctggt
      181 gcacgaggcc ttgtatgttc cctgggtcga tcgtctggta gcacgggtca aaaacggtgc
      241 cacgctcaaa aagcaccttc ttgagagcca taccgccgtc gaggacgtcg gcaagatcgc
      301 cgctgccgcc ggcgtcaaag tactggtgct cagccatctg gtgcccggcg atctcgatgt
      361 gaccgacgac acctggagcg agggcgcgaa gaagaatttt tccggcaaga tcgtcgtcgc
      421 cagggattta atggagctga aactgccggt gtaaccgttc ttcatccctt ccgatcgcgc
      481 cagaattcaa ttgatcgacg ctccccgatg ccggcgcttg accctcggcg cagcctgcgt
      541 tgcgggctcg gtatccttca atgcgccgat ctttcgcagc gccgcctcgg ccgcgcgctc
      601 gccgctttcc catgcgccgt caaccgttcc ccacagcgtc tcgtgggttg cctcgcccgc
      661 gaggaacatg cagccgatcg gctccgtcag gatttttcgc gacggttggg cgcccggcgc
      721 cgcgacagac atcgcgccaa gcgcaaacgg cgcgatatcc cagcgggtcg cgctcgattt
      781 cttgacggcc gcggctgcgt cgctgccgaa cagcttggtc aaccattcca ccgcaaaggc
      841 gatcatggcc ggttcgccct gcgccgcaag atcgcgcccg aacgagcctc caacatcgat
      901 cgagcacaac gacgacgctc cgatgttcgc gaacagcagc cctgtccgct gcgaggtgct
      961 ctgctcgatg atgatttcgt cgcgcgccag cccgagcgga ttgcccggca gctgcagggc
     1021 gatgtgatcg tagctgccga ggctcaattt cgccgcggca tcgagcgagc gtttgggaag
     1081 atccggcgtg aatttgatat tgcccgcggt cagcacgttg ctcgacaccg taatgaccgc
     1141 ggcgcgcgcg gcgatcttgc ccgccggcgt gtccaccgcg acctcgcggc ggtcccaggc
     1201 aatccggctg gcgggcatcg acagcgcgag cgggacctgc tcgccaagct tcgcgatcag
     1261 cgtgccgagg ccctggcggc aggcaatctg cgcgttgttg cgatcctgcc cgcgggcctt
     1321 gtcgatcgcg gagatgtcct tcagatccct gccggcggca ttggcgccga gcgtgaattc
     1381 gaccgttgcc gcccagtcgc cgagatcctt tggcagcgcg gatgcgcaag caatatcagc
     1441 cttgccgcgc gaggcatcgt cgatggcgcg gttggcgcgc accagggccg cgaggaaata
     1501 ctcgatttcg ccggcgcgtg cattgcggcg gccgacgcgg attttctggc ccggcggcgc
     1561 cggataggtt tcgagccccg cattgcgcgc gagtttgatc atcgggttgg tgtcgggatt
     1621 gtgcatccat cgcgcgccgc gatcgaacgg cacgtcgaat gtcctggcgt cggtgaggca
     1681 gcggcctccg atctggccgg tcgcctccac cacgatgacc cgacggttcg cagcctggat
     1741 gcgtcgcgcc gcggcaatgc cggcggcgcc tgcgccgatg acgacaatat cggcctcgcg
     1801 cggcagtggc gccccccatg catcggtgtg cagcgccggt gtcaccgcga gtgccgcgga
     1861 cgccgacagg aagttgcggc gcgtcatagt catgtcatgg tttccgaaaa cattgaaaag
     1921 ccggagaaac acgggagaac agctggtgaa cttgccgcat ccgggcgcgg ccggcaaccg
     1981 tcatggtgaa tcaatcatga ttgatccgcc aaatgcatga actaaattgc aacggctttc
     2041 gaccatgcta aggaagaaga aaaaggcggt cagaacgccc gctgggagaa aatgtgatgg
     2101 gcgttgtgct cgataccatc ggcaagctga tcgcggacta ccttcagaag gaaataccgg
     2161 gctatgagcc gttcacgccg agcgaccccg aacatttgcg cggcgtcatc gag
//
LOCUS       DATNOI010000237        12504 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1463474,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000237 DATNOI010000000
VERSION     DATNOI010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12504
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463474"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /locus_tag="VN968_08945"
     CDS             complement(<1..123)
                     /locus_tag="VN968_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HXL29401.1"
                     /translation="MSAEKTIQHTITNKLREAFAPESLDVTDESHLHEGHAGHRP"
     gene            232..891
                     /locus_tag="VN968_08950"
     CDS             232..891
                     /locus_tag="VN968_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HXL29402.1"
                     /translation="MPIDSSKFFDSIRIKPNKLSAKQQAAAREAAAMCEWPDCKNKGP
                     HRAPKGRANDKEYWHFCLNHVREYNQSYNFFSGMNADAVARYQKDALTGHRPTWKMGA
                     NTSSKKGKGSPEADLEGASDPFSMFSELNGRGRWRPGPGSGDEPKPETRKIFNAERKA
                     LQVMGLGPDATLEVVKAKYKALVKQHHPDANGGDRSTEDRLIEIIKAYNYLKTVVRGA
                     T"
     gene            complement(954..2045)
                     /locus_tag="VN968_08955"
     CDS             complement(954..2045)
                     /locus_tag="VN968_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase/methylcitrate synthase"
                     /protein_id="HXL29403.1"
                     /translation="MNLQLSKSPIGLDGIPAAETVLSHVDGERGELIIAGERVGDLVR
                     KTGFEGVTARLWSGGTGQPIGEAEVRATLGAARERAYARLADLLGITRGLSIVDGFRA
                     AIAGLRAENGLSHEATIVGAFPVIAGALVQRARGGEPVAPDPAASHAADTLSIMLGRK
                     PDAREVAALDAYLVTVCDHGMNASTFTTRVVASTQADLFAAVTAGYCALTGPLHGGAP
                     EPVLEMLDAIGTAERIKPWVDGALSRGERLMGFGHRIYRVRDPRADVLKGAIERLAGG
                     GSGLPFAGEVEAYIRQALRRKNPDRPLDTNVEFFTAILLDALTIPRQAFTPVFAVARA
                     AGWTAHAREQQRTGRLIRPSSAYIGPMPD"
     gene            2118..3341
                     /locus_tag="VN968_08960"
     CDS             2118..3341
                     /locus_tag="VN968_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase family protein"
                     /protein_id="HXL29404.1"
                     /translation="MRESGALYLSAREASAELAISPATLYAYVSRGLIRSEPSSDSRS
                     HRYRAEDVRGLKERRTPSPEPRGLKSFDADLPVMDSAISTITEDGPIYRGVNCIELAE
                     TDRLEHAATLLWDVTGTDPFSSDNCPHVSDEMRGVAEAARNAAPIDRTIAVLALAASA
                     DPRAFTRAADGRALVGGRILRLVVATMLNGISSPKPLHLQIARAWAPGHKHAADLIRR
                     ALVLLADHELNASTFTVRCAASTGLNLYDAVIAGLVALKGPRHGGAGVLAAQLVKMLV
                     DRDVAPIIRERVALGERFAGFGHGVYKHGDPRAHALLDALARAGAAEKLTREIPERIL
                     EATGEFVNIDYALAVLVHTLGLPPGHELVLFAMARTVGWIAHACEQLRHGGLIRPRAR
                     YIGPAPGRSPAFSRP"
     gene            complement(3357..4034)
                     /locus_tag="VN968_08965"
     CDS             complement(3357..4034)
                     /locus_tag="VN968_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXL29405.1"
                     /translation="MTSLIDPLIAFVSAHAWLGYLTLFLAALLEAVPVVGSVVPGSTI
                     ILALSALVPGGELRLVWVMVAAIAGALLGDGSAFWIGHRTQREILSSWPMSNYPRVVA
                     QSEAFFHRWGALAVFFARFVPPIRAFVPITAGALGMSLPRFFTVNIPAILLWAPAHVL
                     PGVLAVSALHRYAGIPHHSGIGKHYWMLTVVVGALVVGLTVWAIRRRKGRAMIEPTAE
                     KVRGDFR"
     gene            4342..5340
                     /gene="cobS"
                     /locus_tag="VN968_08970"
     CDS             4342..5340
                     /gene="cobS"
                     /locus_tag="VN968_08970"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HXL29406.1"
                     /translation="MTTAAMTKAQEPAGLPDMKVSVRQVFGIDSDLEVPAYSEVDPHV
                     PDVDTDYRFDRATTLAILAGFAKNRRVMVTGYHGTGKSTHIEQVAARLNWPCVRVNLD
                     SHISRIDLVGKDSIVVRDGMQVTEFRDGILPWALQHNIALVFDEYDAGRPDVMFVIQR
                     VLEVSGRLTLLDQNKVIKPHPAFRLFSTANTVGLGDTSGLYHGTQQINQGQMDRWSIV
                     TTLNYLAHDEEVEIVLAKAYHYRNQEGRDIVNKMVRLADLTRNAFANGDLSTVMSPRT
                     VITWAENAEIFSDIGFAFRVTFLNKCDELERPLVAEFYQRCFNAELAESSVNVALS"
     gene            5344..5754
                     /locus_tag="VN968_08975"
     CDS             5344..5754
                     /locus_tag="VN968_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL29407.1"
                     /translation="MPSISVERTIGTTKRAVLGGLIGYNTEKMGKQKYKRLAISLRQG
                     DKIVGGIVGEVWTTVLFIQLFWIERKLRGKDYGTKLIGAIEDEAKRFGATRSYLDTMS
                     FQAPGFYRACGYEEFGSIEGYPGGVTRHWFTKSL"
     gene            5751..7649
                     /gene="cobT"
                     /locus_tag="VN968_08980"
     CDS             5751..7649
                     /gene="cobT"
                     /locus_tag="VN968_08980"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HXL29408.1"
                     /translation="MSTSNQKFRTGSKEAPTEPFKRAVTACLRAIAKAPELEVTFAAE
                     RPGLAPGKARLPEPARKMTRRDAAIVRGHADSIALKLACHDPKVHRKLMPGNPQARGV
                     FEAVEQARVEALGSRRMAGVAKNLTAMLDDHFHRGKFDEITDRADAPLSDALAMLVRE
                     RLTGLAPPAAARKMVDLWRPVLEDKIGARLDRLDRLAEDQAKFGDAVHDLLSALELGD
                     DRNAEADDDDNQDDNRDGENDQSGAEGSPDSDAAQEMSADQAQATSDEMSESALESAQ
                     ASTSDTFDDGELGDDETPGEATRPNSRGVNEPRGPEYHAFAPKFDEVIAAEDLCDHDE
                     LERLRSYLDKQLAHLQGIVARLANRLQRRLMAQQNRAWEFDLEEGVLDPARLSRVVTD
                     PYHPLSFMNEKEATFRDTVVTLLLDNSGSMRGRPITVAATCADILARTLERCGVKVEI
                     LGFTTRAWKGGQSREAWLAAGKPPNPGRLNDLRHIIYKSADAPWRRARKNLGLMMREG
                     LLKENIDGEALDWAHKRLLGRAEQRKILMMISDGAPVDDSTLSVNPGNYLERHLRYII
                     EEIETRSPVELIAIGIGHDVTRYYRRAVTIVDAEELGGAITEKLAELFSETHGVAPAR
                     TGRRRLHS"
     gene            7834..8904
                     /locus_tag="VN968_08985"
     CDS             7834..8904
                     /locus_tag="VN968_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HXL29409.1"
                     /translation="MRAPISRRRFLKFTAAGLATTAMPEIAWAQLATEPRPKPLVPNE
                     FSVAEPISIEVNARPIPFFDTRDHARVRFGSLEYRSGLVLTSSFRGFGGLSGLRLDAK
                     GERFISISDHGSWFTGRIVYRGREMTGLADVEAAPLLGADGRPITARGWYDSESIALD
                     GPDVYIGLERANEILRYDFSKGFTRARGEVVPTPPAMRKLPRNKGIEALVMVPKALPL
                     AGTLIALSERGLDAKGNLIAFLIGGKTPGQFGVRRTDNFDISDAVLLSSGALLILERK
                     FSWLSGIGIRIRSIPLNSVAPGALVDGPAIFDADLGHEVDNMEGIDVHVTPEGDTVLT
                     MISDDNFSLLQRTLLLQFKLAE"
     gene            9147..10108
                     /locus_tag="VN968_08990"
                     /pseudo
     CDS             9147..10108
                     /locus_tag="VN968_08990"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
     gene            10199..11311
                     /locus_tag="VN968_08995"
     CDS             10199..11311
                     /locus_tag="VN968_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HXL29410.1"
                     /translation="MSALFSPIKLRSLALPNRIMVAPMCQYSAENGEANDWHFTHINT
                     LALSGAAMFCIEATHVEAIGRITPGCLGLWNDATEAALKPILASVRKHSRIAVAMQLA
                     HAGRKGSSHTPWDGGQQIPLAEGGWQTEGPSAVPHKEGELAPLALDALGLKRVRDAFV
                     AAAKRAERLGLDALELHSAHGYLLHQFLSPISNKRTDQYGGSLQNRMRYPLEVFDAIR
                     AAFPEQKPVGVKVSATDWVEGGWDLAQTIEFAKELKQRGVDWIDASSGGVSPLQKIPL
                     APGYQVPFAQGIKEATGVTTMAVGLITEAKQAEDIVASGKADMVALARGMLYDPRWGW
                     HAAAELGGEVEAPPQYWRSQPSTQKALFGATTFGTR"
     gene            11631..>12504
                     /locus_tag="VN968_09000"
     CDS             11631..>12504
                     /locus_tag="VN968_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXL29411.1"
                     /translation="MMKIICGVDVSKATLDVCIEPGSISGSFKNTTAGIAAPAVFCRQ
                     HRADLVVMEATGGFERRAFLLLSNEGLSCAVTNARNVRQYAESMGVLEKTDRIDARII
                     ARFARDKGLRPMPVPSQAQQRIKALVARLRQVTDDLTVQKQRRSSLLDNAEMLASLDE
                     VIALLKRQSRKLEGEIASTIDDDPLWAQLAETWRSVKGVAGLSKGSDTLKLAGERGFI
                     PMSLNLNPAYVSSHWDSVLAGAARTGRTPSRQDWRPVREVFIADTDEEAWKLSTGDMM
                     GRMMGEYFLPLLGHF"
BASE COUNT         2330 a         3961 c         4041 g         2172 t
ORIGIN      
        1 cggcctgtgg ccggcgtggc cctcatgcag atgtgattcg tccgtgacgt cgaggctttc
       61 cggcgcgaaa gcttcacgca acttgtttgt gatggtatgc tggatggttt tttcggcact
      121 catgcccgcg gcaatacgtc catgaccgcg ttccgtcaat ggcgcatcaa gagcgaggga
      181 ttcgcaatgt caagacttga agcatttcgc attgcgttgt catagtcaga catgccgatt
      241 gattcatcca aattcttcga ctccattcgc atcaagccca acaagctgag cgcgaagcag
      301 caggcggccg cgcgcgaagc agccgcgatg tgcgaatggc ccgactgcaa gaacaagggg
      361 ccgcaccgcg cgcccaaggg ccgcgccaac gacaaggagt actggcactt ctgtctcaat
      421 cacgtccgcg agtacaacca gtcctataat ttcttctccg gcatgaatgc cgacgccgtt
      481 gcccgttacc agaaggatgc gctgaccggc catcgtccga cctggaagat gggcgccaat
      541 accagctcca agaagggcaa gggcagcccg gaagcggatc tggaaggcgc gtccgatccg
      601 ttcagcatgt tcagcgagct caatggccgc ggccgctggc ggcccggtcc gggctcaggc
      661 gacgaaccca agccggaaac ccgaaaaata ttcaacgccg agcgcaaggc gctgcaggtg
      721 atggggctcg gcccggacgc gacgctcgag gtcgtcaagg cgaaatacaa ggcgctggtg
      781 aagcagcacc atccggatgc caatggcggc gaccgctcga ctgaagatcg cctgatcgaa
      841 atcatcaagg cgtataatta tctcaagacc gtcgtgcgcg gcgcaacctg agcatcgccg
      901 ggacgacgca gagagcgcgc gttgcgctct catcttttca tcaagccgtt atctcaatcc
      961 ggcatcgggc cgatataggc tgaacttggc cgtatcagcc ggccggttcg ctgctgctcg
     1021 cgggcgtgcg ccgtccatcc ggcggcgcgg gccaccgcga agaccggcgt gaaggcctgc
     1081 cgcgggatcg tcagggcgtc gagcaggatc gcggtgaaga actccacatt ggtgtcgagc
     1141 ggccggtccg ggtttttccg ccgcagtgct tggcggatat aggcctccac ctcgccagcg
     1201 aacggtagac cgctgccgcc gccggcgagc cgttcgatcg cacccttcag cacatccgcg
     1261 cgcgggtcgc ggacgcgata gatccggtgg ccaaaaccca tcagccgttc gccgcgtgaa
     1321 agcgcgccgt cgacccatgg cttgatgcgc tcggccgtgc cgatggcatc cagcatttcc
     1381 agcaccggct ccggggcgcc gccgtggagc ggcccggtca gcgcgcaata tcccgccgtc
     1441 acggccgcga acaggtcggc ctgggtcgag gccacgacgc gcgtcgtgaa cgtcgaggcg
     1501 ttcatgccgt gatcgcacac cgtcaccaga taggcgtcga gggctgccac ttcgcgcgcg
     1561 tcgggtttgc ggccaagcat gatcgacagc gtatcggcgg catggctggc ggccggatcg
     1621 ggcgcgacgg gttcgccgcc cctggcgcgc tgcaccagtg cgccggcgat caccggaaac
     1681 gccccgacaa tcgtcgcctc atgcgaaagt ccgttttccg cgcgcaatcc ggcgatggcg
     1741 gcccgaaagc catcgacgat cgacaagccc cgggtaatgc cgagcaggtc ggcaagccgg
     1801 gcataggcgc gttcgcgggc cgcgcccaat gtggcgcgca cctcggcctc accgattggc
     1861 tggccggttc cgccgctcca cagccgggcg gtcacgccct cgaagccggt cttgcgtacg
     1921 agatcgccga cgcgctcgcc ggcgatgatc agttcgccac gctcgccatc gacatggctg
     1981 agcacggttt cggcggcggg aatgccgtcc agcccgatcg ggctcttgga aagttgcagg
     2041 ttcatgatcg gtctcctctc ggacctcgaa ggtcgaccct ctcgacagat tgatcaatct
     2101 tgattatgta aatcaatatg agagaatccg gagcacttta cctctccgcg cgggaggctt
     2161 cggccgaact ggcgatctcg cccgccacgc tctacgccta tgtcagtcgc ggattgatcc
     2221 gctcggagcc ttcgtcggat tcgcgaagcc atcgctaccg ggcggaggac gtccgcggcc
     2281 tcaaggaacg gcgcacgccg tcgccggagc cgcgcggtct caagagtttc gacgccgatc
     2341 tgccggtgat ggattcggcg atctcgacca tcaccgagga tggcccgatc taccgcggcg
     2401 tgaattgcat cgagcttgcg gagaccgaca ggctcgaaca tgccgcgacc ttgttgtggg
     2461 atgtgaccgg caccgatccg ttctcgtccg acaattgccc gcatgtgtcc gacgaaatgc
     2521 gcggcgtcgc cgaggccgcg cgaaatgccg cgccgatcga ccgcaccatc gccgtgctgg
     2581 cgctggccgc gagcgccgat ccaagggcct ttacccgcgc cgccgacggc cgcgcgctgg
     2641 tcggcggccg catcctgcgc ctggtggtcg cgactatgct gaatggcata tcatcgccaa
     2701 aaccgctgca tcttcagatc gctagggcat gggccccggg ccataaacat gccgccgatt
     2761 tgatccgccg ggcgctggtg ctgctggccg atcatgaact gaatgcctca acctttaccg
     2821 tgcgctgcgc cgcatccacc gggttgaacc tttatgatgc tgtcattgcc ggcctcgtcg
     2881 cgctgaaagg ccccaggcat ggcggtgccg gagttctggc ggcgcagctc gtcaagatgc
     2941 tggtcgatcg cgatgtggct cccatcatcc gggagcgcgt cgcgctgggc gagcgtttcg
     3001 cgggcttcgg ccacggcgtc tacaagcacg gcgatccgcg cgcgcacgcc ctgctggacg
     3061 cattggcgcg cgcaggtgct gcggagaagc tcacccggga gattccggag cgaatcctcg
     3121 aggcgaccgg cgaattcgtc aatatcgatt atgcgctggc ggtactggtg cacacgctgg
     3181 gcctgccgcc gggccacgaa ctggtgcttt tcgccatggc gcgcacggtc ggatggatcg
     3241 cgcacgcctg cgagcaattg cgccatggcg gcctgatccg cccccgcgcg cgctatatcg
     3301 gcccggcgcc ggggcgcagc ccggccttca gcaggccatg acgccaaggc cgaatcctat
     3361 cgaaaatcgc ctctgacctt ctcggcggtc ggctcgatca tggctctgcc ctttcggcga
     3421 cggatcgccc agaccgtcag ccccacgacc agcgcgccga ccaccaccgt cagcatccaa
     3481 taatgcttgc cgatcccgct atgatgcgga attccggcat atcgatgcag cgccgatacc
     3541 gccagcacgc ccggcagcac atgcgcgggt gcccacagca ggatcgccgg aatgttgacg
     3601 gtgaaaaagc gcggcagcga catgcccaac gcacccgccg taatcggcac gaaggcacga
     3661 attggcggca cgaagcgggc gaaaaacacc gccagcgcgc cccagcgatg gaagaacgcc
     3721 tcgctctgcg cgaccacgcg cggatagttc gacatcggcc aggaactcag gatctcgcgc
     3781 tgggtgcgat gtccgatcca gaacgccgag ccgtcgccga gcagcgcgcc ggcgatcgcc
     3841 gcaaccatca cccagacaag gcgcaactcg ccgccgggca ccagcgcgct cagcgcgagg
     3901 atgatggtcg agcccggaac caccgagccc acgactggaa cggcttccag cagggcggcg
     3961 aggaatagcg tgagatagcc aagccacgcg tgcgccgaga cgaacgcaat caggggatcg
     4021 atcaaggagg tcacaaaatt cctgtgctgg tactgctgtc cggtttgagc ccagacctag
     4081 cataacttcc ggcaggggtc gaaagcgctg tcggagcccc agcatgaggg gtggctaatt
     4141 gtcccggcga aagtacggct cgattcgcag ggcagccctc caaaaaccga agccagcggc
     4201 tatcttgatg ataacgtgac ggaactttga ttgcgccgcg ggcttctgcc ggccggtgct
     4261 ctctgctagg ttcacttgcg cacccatccg cagccaagcc gcaaatctgg tttcgggagc
     4321 gcccgagatc tcggaggatt gatgacgacc gccgccatga ccaaagcgca ggagcccgcc
     4381 ggcttgcccg acatgaaggt gtcggtccgg caagtgttcg gcatcgatag cgatctcgaa
     4441 gtgccggcct attccgaggt cgatccgcat gtgccggacg tcgatacgga ttatcgcttc
     4501 gatcgtgcca ccacgctcgc catcctcgcc ggcttcgcca agaaccgccg cgtcatggtg
     4561 acgggctatc acggtaccgg caagtcgacc catatcgagc aggtcgcggc gcgactgaac
     4621 tggccctgcg tgcgcgtcaa cctcgacagc catatcagcc ggatcgacct ggtcggcaag
     4681 gattccatcg tcgtgcgcga cggcatgcag gtcaccgaat tccgcgacgg cattctgccc
     4741 tgggcgctgc agcacaatat cgcgctggtg ttcgacgaat acgacgccgg ccgtcccgac
     4801 gtgatgttcg tgatccagcg cgtgctggaa gtctccggcc gcctgacgct gctcgaccag
     4861 aacaaagtca taaaaccgca tccggcgttc cggctgttct cgaccgccaa tacggttggc
     4921 ctcggcgata cctcgggcct ctatcatggc acccagcaga tcaaccaggg ccagatggac
     4981 cgctggtcga tcgtcaccac gctgaattat ctggcgcatg acgaggaagt cgagatcgtg
     5041 ctggccaagg cctaccacta ccgcaaccag gaaggccgcg acatcgtcaa caagatggtg
     5101 cggcttgcgg atcttacccg caacgccttt gccaatggcg atctgtcgac ggtgatgagc
     5161 ccgcgcacgg tgatcacctg ggcggagaac gccgagattt tctccgacat cggctttgcg
     5221 ttccgcgtca ccttcctcaa caagtgcgac gagctggaac ggccgctggt cgccgaattc
     5281 tatcagcgct gtttcaatgc cgagctcgcg gagtcttcgg tgaacgtggc gctcagctag
     5341 tccatgccgt ccatttccgt cgagcgcacc attggaacga ccaaaagggc ggtgctcgga
     5401 ggactgatcg gctacaacac cgagaagatg gggaagcaga aatacaagcg cctcgccatc
     5461 tcgctacgcc agggcgacaa gatcgtcggt gggattgttg gagaggtctg gaccaccgtg
     5521 ctgttcatac agttgttctg gatagagcgg aagcttcgcg gcaaggacta tggaacaaag
     5581 ctgatagggg cgattgagga tgaagcgaag cgttttgggg ccacgcggtc ctatctggac
     5641 acgatgagct ttcaggcgcc gggcttctac cgggcatgcg gttatgagga attcggttcg
     5701 atcgagggtt atccgggcgg cgtaacgcgt cattggttta caaaatcgct atgagcacat
     5761 ccaaccaaaa attccggacc ggatcgaagg aagcgccgac cgagccgttc aagcgcgcgg
     5821 tgaccgcctg cctgcgtgca atcgcaaagg cgccggagct tgaagtgacg ttcgccgccg
     5881 agcgcccggg tctcgcgccc ggcaaggcgc ggctgccgga gccggcccgc aagatgacta
     5941 ggcgggatgc ggcgatcgtt cgcggccacg ccgattcgat cgcgctgaaa ctcgcctgtc
     6001 acgatcccaa agtgcaccgc aagctgatgc cgggcaatcc gcaggcgcgc ggcgtgttcg
     6061 aggcggtgga gcaggcccgg gtcgaggcgc tcggctcgcg gcggatggcg ggggttgcga
     6121 aaaaccttac cgcgatgctc gacgatcatt tccatcgcgg caagttcgac gagatcaccg
     6181 accgcgccga cgcgccgcta tcggacgcgc tggcgatgct ggtgcgcgag cgcctcaccg
     6241 ggctagcgcc gccggcagcc gcccgcaaga tggtcgacct ctggcgtccg gtgctggaag
     6301 acaagatcgg cgcgcggctc gaccggctcg atcgccttgc cgaggatcag gcgaaattcg
     6361 gcgacgcggt gcatgatctg ttgtccgcgc tcgaactcgg cgacgaccgc aacgccgagg
     6421 ccgacgatga cgacaaccag gacgacaacc gcgacggcga aaacgaccag tccggcgcgg
     6481 aaggctcgcc cgacagcgac gcagcccagg aaatgagcgc cgatcaggcg caggccactt
     6541 ccgacgagat gtcggagagc gcgctggaaa gcgcgcaggc ctccaccagc gacaccttcg
     6601 acgacggcga actcggcgac gacgagacac cgggcgaagc gacgcggccg aactcgcgcg
     6661 gcgtcaacga gccgcgcggg ccggaatatc acgcgttcgc gccgaaattc gacgaggtca
     6721 tcgcggccga ggatctttgc gaccatgacg agctggagcg cctgcgcagc tatctcgaca
     6781 agcagctcgc gcatctgcag ggcatcgtcg cgcggctcgc taaccggctg cagcgccgcc
     6841 tgatggcgca gcagaaccgc gcctgggaat tcgatctcga ggaaggcgtg ctcgatccgg
     6901 cgcggctgtc gcgcgtggtg accgatccct atcatccgct gtcgttcatg aacgagaagg
     6961 aagcgacctt ccgcgatacc gtggtgacgc tgctcttgga caattccggc tcgatgcgcg
     7021 ggcggccgat cacggtggcg gcgacctgtg ccgatattct ggcgcggacg ctggagcgtt
     7081 gcggcgtcaa ggtcgaaatc cttggcttca cgacacgggc ctggaagggc ggccaatccc
     7141 gcgaagcgtg gctcgccgcc ggcaagccgc ccaatcccgg acgcctcaac gatctccggc
     7201 acatcatcta caaatcggcc gacgcgccgt ggcggcgcgc gcgcaagaat ttggggctga
     7261 tgatgcgcga gggactgttg aaggaaaaca tcgacggcga agcgctggac tgggcgcaca
     7321 agcggctgct cgggcgcgcc gaacagcgca agatcctgat gatgatttct gacggtgcgc
     7381 cggtcgatga ttccacgctg tcggtcaatc ccggcaatta tctggagcgc catctgcgct
     7441 acatcatcga ggaaatcgag acccgctcgc cggtcgagct gatcgcgatc ggcatcggtc
     7501 acgacgttac ccgctattat cgccgcgcgg tgacgatcgt cgacgccgaa gaactcggcg
     7561 gtgccatcac cgagaagctc gccgaactgt tcagcgagac ccacggtgtt gcccccgcca
     7621 ggacaggccg ccgcagattg cattcgtaag atttcgatct atgaagttcg gatcggaagc
     7681 cgctacaggg acacttcacc ttctcccatc gaagtcggat atatccgact tcgacagatt
     7741 tggataccca actcgggtaa acccgagttg ggtgggagag ggagcgcgct tgcgttgctg
     7801 tgaggtcgaa taatgcctcg atagatggat tccatgcgcg caccgatcag ccgccgccgc
     7861 ttcctgaaat tcacggcggc agggcttgcg acgactgcca tgccggagat cgcgtgggcg
     7921 cagctcgcca cggagccgcg gccaaagccg ctggtcccga acgagttctc ggtcgccgag
     7981 cccatctcga tcgaggtcaa cgcccggccg attccctttt tcgacacgcg cgatcacgcg
     8041 cgtgtccggt tcgggtcgct ggaatatcgc agcgggctgg tgctgacctc gagctttcgc
     8101 ggctttggcg ggctgtcggg gctgcggctc gacgccaagg gcgagcgttt catttcgatc
     8161 agcgaccacg gcagctggtt caccggccgt atcgtctatc ggggccgcga gatgaccggg
     8221 cttgccgatg tcgaggccgc cccgctgctc ggcgccgacg gccggccgat caccgcacgc
     8281 ggctggtacg actcggagtc gatcgcgctc gatggtccgg acgtgtatat cggcctcgaa
     8341 cgcgccaacg agattctgcg ttacgatttc agcaaggggt ttacccgggc gcgcggcgag
     8401 gtggtgccga cgccgccggc gatgcgcaag ctgcctcgca acaaggggat cgaggcgctg
     8461 gtgatggtgc caaaggccct gccgctggcc ggcacgctga tcgcgctttc ggagcgcggt
     8521 cttgacgcga agggcaacct gatcgcattc ctgatcggcg gcaagacgcc tggccagttc
     8581 ggggtccgcc gcaccgacaa cttcgacatc agcgatgcgg tgctgctgtc ctcgggtgca
     8641 ctcctgatac tggaacggaa gttttcgtgg ctctccggga taggcatccg gatccgcagc
     8701 attccgctca actcggtggc gccgggggca ctggtcgatg gccccgcgat tttcgatgcc
     8761 gatctcggtc acgaggtcga caacatggaa ggcatcgacg tccacgtcac gcccgagggc
     8821 gacactgtgc tgacgatgat ctccgacgat aatttctcgc tgctccagcg cacgctgttg
     8881 ctgcagttca aactggcgga gtgattcttg tcggcccggc gaacgcggcg atgacgcggg
     8941 ttttgcgcgg gcctaaatta tgtcgtcccg gcgaacgccg ggacccatac gccgtgtcct
     9001 ctcgtttgag cagtggggca atcaacaagg ccagtggtta tgggtccccg cgttcgcggg
     9061 gacgacagga acggttcttg caacgctgct gccggccgtc acatgatgga taagctcatc
     9121 acggcggcga tgaaaccgga gcgtcgatgc gcaagtccat tccattgatt tcaattctcg
     9181 cgagcttcct gtttgccttt gcggcgtgcg cggaaaactg gccgtcgcga ttgatcaagg
     9241 cgatcatccc gttcggcgcc ggcagcgccg ccgatgtggt gccgcggctg gtgttcgacc
     9301 ggctcgcggc ggaactcggt caacccatcg tgatcgagaa tcgcgccggc gccggcggca
     9361 tgctcggcac cgcggcggtg gcgaaggccg aacccgacgg ctacagcatt cttgcccatt
     9421 cttccgcgct gacgatcgcg ccgtcgattt ttccgaattt cacattcgat gccaccaggg
     9481 acctcgcgtc ggtcttgatg atcggctcca gcgccaatgt gatgatcgtg ccggtctcgc
     9541 ggccctggaa gaccgtgcag gatttcatcg ctgacgccaa agcgaaaccc ggctcgatct
     9601 cgttcggctc ggtcggcatc ggcagcgcgg tgcatatcag cgccgagaat tccgcctcgc
     9661 cgccggcatc gagaccaccc acgtacccta tcgtggcggt tctgaagtca tcaccgatat
     9721 tctcggggct cggatcgatt tctatttctg tccgctcgcg accgcgctgc ccctgatccg
     9781 cgaaggtcgg gtgcgcgcgc ttttggtgtc gacctcaagg cgtgtcgccg atctgcccga
     9841 cgtgccgaca ccgcaggagg ccgggctgaa agatgccgac agcgcgatct ggttcggcgt
     9901 gttcatgccg tcgaagacgc cgcgcgacat catcgagaaa tttcatgctg ccggggtcag
     9961 ggtgctgtcg gaacccgcga tgcaggaaaa cctgaaaaaa ctcggcgtcg aagcgttgcc
    10021 gatgacgcct gcggaaatgg acgatctcgt caagcgcgag accgcggcca ccatggaagt
    10081 gatcaaggcc gccgggatca agccataggg cgatcaagca agggtgccat gcggttgagg
    10141 aaagggcggc gcctcgctag atagagggca acgaatttcc ctcccaccgg acatccccat
    10201 gagtgccctg ttctctccga tcaaactgcg cagccttgcg cttcccaacc gcatcatggt
    10261 ggcaccgatg tgccagtact ctgctgaaaa cggcgaggcc aatgactggc atttcaccca
    10321 tatcaacacg ctcgcgctgt cgggcgctgc gatgttctgt atcgaggcga cccacgtgga
    10381 agcgatcggc cgcattacgc cgggatgtct cggcctgtgg aatgacgcca ccgaggcggc
    10441 gctgaagccg atcctggcct ccgtgcgcaa gcattccagg atcgcggtgg cgatgcagct
    10501 cgcccatgcc ggccgcaagg gatcgagcca tacgccatgg gatggcgggc agcaaatccc
    10561 gctcgccgaa ggcggctggc agacggaagg gccgtccgcg gtgccgcaca aggagggcga
    10621 actggcgccg ctcgcgctcg atgcgctggg gctaaagcgc gtgcgcgatg cattcgtggc
    10681 ggccgccaag cgcgccgagc ggctaggcct cgatgcgctc gaactgcact ctgcccacgg
    10741 ctatctgctg catcagtttc tgtcgccgat ctccaacaaa cgcaccgatc agtatggcgg
    10801 ctctttgcaa aaccgcatgc gctatccgct ggaagtgttc gacgcaattc gcgcggcgtt
    10861 cccggagcaa aagccggtcg gcgtcaaggt gtcggcgacc gactgggtcg agggcggctg
    10921 ggatctcgcg cagaccatcg aatttgccaa ggagctgaaa cagcgcggcg tcgactggat
    10981 cgatgcgtcc tccggcggcg tgtcgccgct gcagaaaata ccgcttgcgc ccggctatca
    11041 ggtgccgttc gcgcaaggca tcaaggaggc caccggcgtg accaccatgg cggtcggtct
    11101 gatcaccgaa gccaagcagg ccgaggacat cgtcgcgtcc ggtaaggccg acatggtcgc
    11161 gctggcgcgc ggcatgctct acgacccgcg ctggggctgg cacgccgcgg ccgaactcgg
    11221 cggcgaggtc gaagcgccgc cgcaatactg gcgctcgcag ccttcgaccc agaaggcgct
    11281 gttcggcgcc acgacgttcg ggacgaggta gaagggagaa ggtggccgta ggcggccttt
    11341 ggccgccgtt ctttaatagg acgccgatcc tttgcatcgg ctatgcgaag tccgtgccgg
    11401 atgacgggtc acgtcccatc gataggccga gaccccttgt atctgcatat cgagcaaaat
    11461 gtgcaatgga agcgactgag cgctttggcc ggtggccacc ggccaaagcg cgcggcgatg
    11521 tcgcccgata cccccttggc aacaaccgtg ggtgagcatg ggatcttcgc cgttgtcacg
    11581 cacaaccgaa cagtcggtgg attacgatcg tacgcacaag gcaggttacc gtgatgaaga
    11641 tcatttgtgg agtggatgtt tcgaaggcta cgctcgatgt ttgcatcgag cctggctcga
    11701 tctctggcag cttcaagaat accacagctg ggatcgcggc gccggcggtg ttctgccgcc
    11761 agcaccgggc ggatctggtg gtgatggagg cgaccggggg ctttgaacgc agggcgttcc
    11821 tgctgctgtc gaacgagggc ctgtcctgtg ccgtgaccaa tgcgcgcaat gtccggcaat
    11881 atgccgagtc gatgggcgtg ctggagaaga cggatcggat cgatgccagg attatcgccc
    11941 gcttcgcccg cgacaagggg ttgcggccga tgccggtgcc gagccaagcc cagcagcgca
    12001 tcaaggccct tgtggcaagg ctcaggcagg tcaccgatga tcttaccgtg cagaagcagc
    12061 gccgctccag tctgctcgat aacgccgaga tgctggccag cctcgatgag gtgatcgcct
    12121 tgctgaagcg gcagtcgcgc aagctggagg gcgaaattgc ttccacgatt gatgacgatc
    12181 cgttgtgggc gcagctcgcc gaaacgtggc gttcggtgaa gggcgtggcc ggcttgagca
    12241 aggggtcgga caccctgaaa ctcgcgggtg aacgcggctt cattccgatg agcctcaacc
    12301 tcaacccggc ctacgtttcc agtcattggg attcggtact ggccggcgcc gcgcggaccg
    12361 ggcgcacgcc gagccggcag gactggcggc cggtgcgcga ggtgtttatc gccgataccg
    12421 acgaggaggc ctggaagctt tcgaccggcg acatgatggg ccggatgatg ggcgaatatt
    12481 tcctgccgct gctcggtcac ttcg
//
LOCUS       DATNOI010000238         3019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_493295,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000238 DATNOI010000000
VERSION     DATNOI010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493295"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1487
                     /gene="trmFO"
                     /locus_tag="VN968_09005"
     CDS             54..1487
                     /gene="trmFO"
                     /locus_tag="VN968_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HXL29412.1"
                     /translation="MTGIKTSSESVHIVGAGLAGSEAAWQVARAGVRVILHEMRPGRM
                     TEAHRTDGLAELVCSNSFRSDDAANNAVGLLHAEMRRLGSLIMRAADANQVPAGGALA
                     VDRDRFSAAVTKALQDHPLIEIQRAEIDGLPPADWRNVIVATGPLTSAPLADAIREIT
                     DEDALAFFDAIAPIVHRDSIDMSAAWFQSRYDKVGPGGTGADYINCPMTRDQYDGFVD
                     ALIAGEKTDFKDWETNTPYFDGCLPIEVMAERGRETLRHGPMKPVGLTNPHDPTVKAY
                     AIVQLRQDNKLGTLYNMVGFQTKLKHGAQQRVFRTIPGLENAEFVRLGGLHRNTFLNS
                     PKLLDAQLRLRAEPRLRFAGQMTGCEGYVESASIGLMAGLYAAADARSASLAPPPATT
                     ALGSLLGHITGGHIETIDAGQRSFQPMNINFGLFPPLASPPTKKPDGTRLRGNDKTIA
                     KKQAMSARALSDLDRWIADNLNVAAAA"
     gene            1495..2625
                     /locus_tag="VN968_09010"
     CDS             1495..2625
                     /locus_tag="VN968_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HXL29413.1"
                     /translation="MSLPRDDAAVLSARWIEGVLLKRDVFSTVERGRFRSEAGEVDAV
                     LRRLDSVPWWSYIPARHLFARERRALALARDLRVGPELLWAGRQALVRGFINGVALHL
                     AKPTGDVAYFRSAKQALRKLHRAGICHNDLAKEQNWLRGSDGNAYVTDFQLAACFKTR
                     GRLFRIAAYEDLRHLLKHKRSYAPQALTPKERKILARKSFVASIWLLTGKKIYRAITR
                     GLFNFTDREGGGRRLVNDAPVLIDLIRNNPGVRDTAIVAFADRRTGVGLYAFVEADQT
                     ALEAQLGSELAAAKGVRPPEHIQVVRALPRDAAGKVRTEVLQLVAMNQLDLIEPMMTS
                     EADRAFLKNILEARKNLRDRFNFEAADLVPRPATDVITRKGG"
     gene            complement(2737..>3019)
                     /locus_tag="VN968_09015"
     CDS             complement(2737..>3019)
                     /locus_tag="VN968_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HXL29414.1"
                     /translation="SGMEEVFAGRQTAKLRAVLHQLVGDAQGHLKTAFALLATVPPRV
                     RPVFLPLKLVARELMRMSRADSDPFAPRVPSRLRTLWTLWRASRSREFS"
BASE COUNT          553 a         1029 c          934 g          503 t
ORIGIN      
        1 acaagccgct tcgcgcattc caacgcgccg gaaaacgcgc tacgcctgtg ctcatgacag
       61 gcatcaaaac ctcctccgaa tcagtgcata tcgtcggcgc gggcctcgcc ggttccgaag
      121 cggcctggca ggtcgcccgt gcgggcgtcc gcgtcatcct tcatgaaatg cgccccggcc
      181 ggatgaccga ggcgcatcgt accgacggcc tcgccgaact cgtgtgctcc aattcgtttc
      241 gctccgacga tgccgccaat aacgcggtcg ggctgctgca tgccgaaatg cgccgcctgg
      301 gctcgctgat catgcgcgcc gccgacgcca atcaggttcc cgccggcggc gccttggccg
      361 tcgaccgcga ccgattctcc gctgccgtca ccaaggccct ccaggatcac cccctgattg
      421 aaatccagcg cgccgaaatc gacggcctgc cgcccgcgga ctggcgcaac gtcattgtcg
      481 cgaccgggcc cttgacctcc gcgccgctgg cggatgcgat ccgcgaaatc accgacgagg
      541 atgcgctggc attcttcgat gcgatcgccc cgatcgtgca ccgggattcc atcgacatgt
      601 cggcggcctg gttccagtcg cgctacgaca aggttggccc cggcggtacc ggcgccgatt
      661 acatcaattg cccgatgacc agggaccaat atgacggctt cgtcgacgcc ctgattgcgg
      721 gcgagaagac cgacttcaag gactgggaaa ccaatacgcc ctatttcgac ggctgcctgc
      781 cgatcgaggt gatggccgag cgcggccgcg agacgctgcg ccacgggccg atgaagccgg
      841 tcggattgac caatccgcat gatccgaccg tgaaggccta cgccatcgtg cagctgcgcc
      901 aggacaacaa gcttggcacg ctctacaaca tggtcggctt ccagaccaag ctcaagcacg
      961 gcgcccagca gcgcgtcttc cgcaccattc ccgggctcga gaacgccgaa ttcgtccggc
     1021 tcgggggcct gcatcgcaac accttcctga attcgccaaa actgctggat gcgcaattgc
     1081 ggctgcgggc cgaaccgcgg ctgcgcttcg ctggacaaat gaccggctgc gagggctatg
     1141 tggaatccgc gagcattggt ttgatggccg ggctttacgc cgcggccgat gcacgctcgg
     1201 cgtcgctggc gccgccgcca gcgacgacgg ccctgggatc gctgctcggc cacatcaccg
     1261 gcggccatat cgagaccatc gatgcaggtc agcgctcgtt ccagccgatg aatatcaact
     1321 ttggactttt tccgccactc gccagtccgc cgacaaaaaa gcccgatggc acgcggctgc
     1381 gtggcaacga caagaccatt gcgaagaagc aggcgatgag cgcccgcgcg ctttcggacc
     1441 tcgatcgctg gattgccgac aacctcaacg tagcggcggc ggcgtgagcg tttcatgagt
     1501 ttgcccaggg atgacgccgc ggttctgtcg gcgcgatgga tcgaaggcgt gttattgaag
     1561 cgcgacgtgt tctccaccgt cgagcgcggc cgttttcgca gcgaggcagg cgaagtcgat
     1621 gccgtgctgc gccggctcga ttccgtgccg tggtggtcct acatcccggc gcgccacctg
     1681 ttcgcacgcg agcgccgcgc gctggcgctg gcgcgcgacc tgcgagtcgg gccggaactg
     1741 ctgtgggccg ggcggcaggc gctggtgcgc ggattcatca acggcgtggc gctgcatctg
     1801 gcaaaaccca ccggcgacgt cgcctatttt cgttccgcca agcaggcgct gcgcaagctg
     1861 catcgcgccg gcatctgcca caacgatctc gccaaggagc agaactggct gcgcggcagc
     1921 gatgggaacg cctatgtgac ggacttccag ctcgcagcct gcttcaagac gcgcggccgg
     1981 ctgttccgca tcgcggccta cgaagatctc cgccatctcc tgaagcacaa gcgcagctac
     2041 gcgccgcaag cgctgacgcc aaaggaacga aagatcctcg cgcgcaaatc gtttgtcgcc
     2101 agcatctggc ttctgaccgg caagaagatc taccgcgcca tcacccgggg actgttcaac
     2161 tttaccgatc gcgagggcgg cggccggcgg ctcgtcaatg acgcgccggt gctgatcgat
     2221 ctgatccgga acaatcccgg tgtccgcgat accgcgattg tcgcgttcgc cgaccgccgc
     2281 accggtgtcg ggctttatgc ctttgtcgag gccgaccaga ccgcgctcga agcgcaactg
     2341 ggcagcgaac ttgcggcggc gaagggcgtg aggccgcccg aacatatcca ggtggttcgg
     2401 gccttgccgc gcgatgccgc cggaaaggtt cgtaccgagg tgctgcagct tgtcgcgatg
     2461 aaccagctcg acctgatcga acccatgatg accagcgagg ccgaccgtgc gttcctgaaa
     2521 aatattctgg aagcgcgcaa gaacctgcgg gaccggttca atttcgaggc ggccgatctg
     2581 gtgccgcggc cagccacgga cgtcatcacc cgcaaaggcg ggtgatccag tattccgtga
     2641 cactgctggt agaatcgaga agccacggcg tactggatac ccacattcgc gggtatgacg
     2701 accgggtgtg acgcagcgcc ctgcgccgcc ttcgtcctac gaaaactccc gcgaccgtga
     2761 cgcccgccac aacgtccata gcgtccgcaa tctcgaaggc acccgcggcg cgaacggatc
     2821 agagtccgcg cgcgacattc gcatcagctc gcgggcaacc agcttgagcg gcaggaacac
     2881 cggcctcacc cgcggcggca cggtcgcaag cagcgcgaag gcggtcttca aatgcccctg
     2941 cgcatcgccg accagctgat gcagcaccgc acgcagctta gcggtttgcc tgcccgcaaa
     3001 cacctcttcc atcccgctg
//
LOCUS       DATNOI010000239         8229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_604156,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000239 DATNOI010000000
VERSION     DATNOI010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604156"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VN968_09020"
     CDS             <1..1248
                     /locus_tag="VN968_09020"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HXL29415.1"
                     /translation="TVIATSPLHMRVKLNMTPEQVIETSIANVARARNHIDDVEWSAE
                     DGTRSEMEFLCRIVEAVIKAGATTVNIPDTVGYTVPEEYTNFMRTLIERVPNSDKAVF
                     SVHCHNDLGMAVANSLAGIAGGARQIECTVNGIGERAGNAALEEVVMAINIRNDVFPW
                     WNKIDTTMLTRASKLVSAATSFPVQYNKAIVGRNAFAHESGIHQDGVLKDASTYEIMR
                     PEMVGLKKSSLVLGKHSGRHAFVHKLEEMGYKLGANQLEDAFVRMKALADRKKDIYDE
                     DIEALVDQEIAASHDRIKLTSLTVIAGTHGPQRATMKLDVDGQIKIEEAEGNGPVDAV
                     FNCIKLLVPHEAKLELYQVHAVTEGTDAQAEVSVRLAHEGRSMTSKASDPDTLVASAK
                     AYLGALNKIVMKRQRDIPAAAAS"
     gene            1722..2723
                     /locus_tag="VN968_09025"
     CDS             1722..2723
                     /locus_tag="VN968_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXL29416.1"
                     /translation="MHKLILAAASVAALALAGPASAQSPIVIKFSHVVASNTPKGAAA
                     EKFKELAEKYTDGKVKVEIYPNSTLYKDKEELEALQLGAVQMLAPSNSKFGPIGVREF
                     EVFDLPYVLPDLATLRKVTDGPLGAKLLKLLEPKGMTGLAYWDNGFKEMTANKKLVTP
                     DDYKSLKFRIQSSKVLEAQFRSLGSIPQVMAFGEVYQALQTGVVDGQENTWSNIYTQK
                     MHEVQKYATITNHGYIGYVVVTNKKFWDGLPADIRTALEKAMKEATAFGNGQSAKENE
                     DALELIKKSGKTEIVSLTPAQDAAMRKAMDPVYKDVASRVGQPLIDAFIKETGGVSH"
     gene            2864..3433
                     /locus_tag="VN968_09030"
     CDS             2864..3433
                     /locus_tag="VN968_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXL29417.1"
                     /translation="MLRILDRLEEVIITSLMAAATILIFVSVVHRFGTGVPFLYPYLI
                     AIHISWAQELCIYMFIWMAKFGAAYGVRTGIHVGVDVLINQLNPRWQKRVILFGLCGG
                     ALFTFIVGSMGAKFVFELMSTDQVSPDLELPSWLVYACIPLGSYLMCFRFLQVSWTYF
                     RTGDLPHHDATHVDGIEVSSKAPVALGEA"
     gene            3433..4719
                     /locus_tag="VN968_09035"
     CDS             3433..4719
                     /locus_tag="VN968_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXL29418.1"
                     /translation="MSAALIFGLLFALMLTGMPISISLGLTVLTFLFTMTEVPIESVG
                     LKLFSGLDNFGIMAIPFFILAGTFLTRGGVARRMIAFTTSLVGHLPGGLGLAGVAACA
                     MFAAISGSSVATVVAIGSIMMPAMVEHGYPRRFGVGVISTSGALGILVPPSIILVLYG
                     VSTNTSIGALFMAGIVPGIMLASMLAVVTWFIAWRNGYPRMQRATFREQWTAFRESVW
                     GLLLIVIVLGGIYGGIFTPTEAAAVAAVYAFFITVFVYKELKLREVSKVLLQAASMSS
                     MLLYIITNAVLFSFLMTHEQIPQEMAAWIIDKNFSLWMFLLVVNILLLMAGNVMDPSS
                     ILLIMAPILFPLATKIGIHPVHLGVLMIVNTEVGLCHPPVGLNLYIASGIAKMGISEI
                     TIAVLPWLCAMLLFLGLITYIPEISLWLPRLLGMIS"
     gene            4916..6514
                     /locus_tag="VN968_09040"
     CDS             4916..6514
                     /locus_tag="VN968_09040"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXL29419.1"
                     /translation="MSPSKGTLLGACLLASLLAVGPMAHAQRATEAAAVDQSTQSLPP
                     VQRSLQTAARPATPANPLGGPEQRVALVIGNSNYQNVSQLSNPANDAKSVAQLLNAAG
                     FEVISATDLSHNEMIQVVQDFSSKIADRGPNTVAMVYYAGHGVQLAGENYLVPVDARI
                     SSETDLVSGSVRLVDMMATLDAIPSRLRIAILDACRNNPFPSLNEAGRGLAIVDAPNG
                     SIVAYSTAPGSEALDGVGDHSPYTAAFLRLAHEKNLPIEQLFKRIRLDVNNSTGGQQT
                     PWESSSLTSDFYFFGDTALAATRLPAQEKAVYTADDLPSRASRAAYDYVLAENSIEHY
                     QEFIQLYPHDPLCYRIRALLDSLMQAKAWHNAVLTNSTVAYQSFYDKFASSPYAQSAL
                     KLAQQPKMVPLSQPTHIMVPASLKFGGLGSGNRNAAPGKIVNLPVGGLNKSDALQNSK
                     FDRAVSRIDRRPAKSFAQIRDRQGPMRMSRSNFGRPPRSGNLRFASTQFHGSGFRHQF
                     RSGGGFGGHGFGGGGFGGGGFGRR"
     gene            6742..7308
                     /locus_tag="VN968_09045"
     CDS             6742..7308
                     /locus_tag="VN968_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HXL29420.1"
                     /translation="MRRFAIAGTAALAIAASTAVYAQHHRWFYEHVRMSPEDRAAFTD
                     ARIAAVKAGLKLTPDQEKLWPPVETAVRDFAKLRIDRANARMNAKPDDSQDAQKQDSQ
                     NLPDPVARLRERADAMATSAAAMKRIADAADPLYKTLDDSQKRRLAILTRMDGRFGGG
                     DWRHHGFMRGMDRDDGDRDGGRGGPERL"
     gene            7493..7568
                     /locus_tag="VN968_09050"
     tRNA            7493..7568
                     /locus_tag="VN968_09050"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7526..7528,aa:Lys,seq:ctt)
BASE COUNT         1598 a         2442 c         2517 g         1672 t
ORIGIN      
        1 accgtgatcg cgacttctcc gctgcatatg cgcgtaaaac tcaacatgac cccggagcag
       61 gtgatcgaga cttcgatcgc caacgtcgcc cgcgcgcgca accacatcga cgacgtcgaa
      121 tggtcggccg aggacggcac ccgcagcgaa atggagtttt tgtgccggat cgtcgaggcc
      181 gtgatcaagg ccggcgcgac cacggtcaat attcccgaca cggtcggcta taccgtgccc
      241 gaggaataca cgaacttcat gcgcaccctg atcgaacggg tgccaaactc cgacaaggcc
      301 gtattctcgg tgcattgtca caacgacctc ggcatggcgg tggcgaattc gctggcgggc
      361 atcgccggcg gcgcgcggca gatcgaatgc accgtcaacg gcatcggcga gcgggcaggc
      421 aatgccgcgc tcgaggaagt cgtgatggcc atcaacattc gaaacgacgt gtttccctgg
      481 tggaacaaga tcgacaccac catgctgacg cgggcctcga aactggtatc ggcggcgacg
      541 tcgtttccgg tgcagtacaa caaggcgatc gtcggccgca atgcattcgc gcacgagagc
      601 ggcatccatc aggacggcgt gctcaaggac gcatcgacct atgagatcat gcggccggaa
      661 atggtcggct tgaaaaaatc gtcgctggtg ctcggcaagc attccgggcg tcatgcgttc
      721 gtgcacaagc tggaggagat gggctacaag ctcggcgcca accagctgga agacgcgttc
      781 gtgcggatga aggcgctcgc cgaccgcaag aaggacatct acgacgagga catcgaggcg
      841 ctggtcgatc aggaaatcgc agcctcccat gaccgcatca agctgacctc gctcacggtg
      901 atcgcgggca cccatggtcc gcagcgcgcc accatgaagc tcgatgtcga cggccagatc
      961 aagatcgagg aagcggaggg caacggtccg gtcgatgcgg tgttcaactg catcaagctc
     1021 ttggtgccgc acgaggcgaa gctggaactg tatcaggtcc acgccgtcac cgaaggcacc
     1081 gacgcgcagg ccgaggtttc ggtgcggctc gcccatgagg gccgctcgat gacctcaaag
     1141 gcttccgatc cggacacgct ggtggcctcg gccaaggcct atctcggggc gctcaacaag
     1201 atcgtcatga agcgccagcg cgatattccg gcggcggcag cgagctgatt gcttcagatg
     1261 cagcgaagtc aaacgcggtg cttgggcgcc gcgtttttgt ttcggctcgt cctcgcgttt
     1321 aattggaatg aaccttcggt tgatccggcc gcgcgaccaa tcggcccggc ggaaactcaa
     1381 gatcgtcgtg cgcgatgtcc agcacgggcc ggcttgtgct cgattatctc cgcgccgcgg
     1441 agcggggcga gcaatccgcc tagagaaagc aggggcgtcg aggatgccgg ccgagttcga
     1501 ggatgaatcg ccaaatggac tcaatcgtcg atggtcgtgc gcaactggcg caatcggcca
     1561 ggaacgacac catgtcgcgt attgcagtgc agcagaactc acaaaacagc cctgctaaag
     1621 gggcaatggc gtttcggtcg gcttctctgt attggtgcgc cttgaatagg gcgtcgcggc
     1681 cgcgccgttg gcgcgcttcg cagagtcacg cgggaggaaa catgcataaa ttaattctgg
     1741 ctgctgcgtc ggtcgcagca ttggctctgg caggaccggc gtcggcgcaa tcgccgatcg
     1801 tcatcaagtt cagccacgtg gtggcttcga acacgccgaa gggcgccgcc gccgagaagt
     1861 tcaaggaact cgcggagaaa tacaccgacg gcaaggtcaa ggtcgagatc tatccgaatt
     1921 cgacgctcta caaggacaag gaagaactgg aagcgctgca gctcggcgct gtgcagatgc
     1981 tggcgccgtc gaattccaag ttcggtccga tcggcgtgcg cgaattcgag gtgttcgatc
     2041 ttccctatgt tctgccggat ctcgccacct tgcgcaaagt caccgacggg ccgctcggcg
     2101 cgaaactgct gaagctgctc gagcccaagg gaatgaccgg tctcgcttat tgggacaacg
     2161 gcttcaagga gatgaccgcg aacaagaagc tggtcacgcc cgacgactac aagagcctga
     2221 aattccgcat ccagtcgtcg aaggttcttg aagcgcagtt ccgctcgctg ggttcgatcc
     2281 cgcaggtgat ggcgttcggc gaggtgtacc aggcgctgca gaccggcgtg gtcgatggcc
     2341 aggaaaacac ctggtcgaat atctacaccc agaaaatgca cgaggtgcag aagtacgcca
     2401 caatcaccaa ccacggctac atcggctatg tggtggtcac gaacaagaag ttctgggacg
     2461 ggcttcccgc tgatattcgc accgcactcg agaaggcgat gaaggaagcc accgctttcg
     2521 gcaacgggca gtcggcgaag gaaaacgagg acgcgctcga actgatcaag aagtccggca
     2581 agaccgaaat cgtcagcttg acgccggcgc aagatgccgc catgcgcaag gcgatggacc
     2641 cggtttacaa ggacgttgcg agccgggttg gtcaaccctt gatcgatgcg ttcatcaagg
     2701 aaaccggcgg agtcagccac taacacaatg ccggctctcg cgaaaagctg cgagagctcg
     2761 cttttcattt gaacggcgtc ggaccgcggg gaccgaccga cgccggttcg gtagatcatc
     2821 ggcgacgcgc ccgaaagttc acgggggcgt atgaggggga gagatgcttc gtatccttga
     2881 tcggcttgag gaagtaataa tcacctcgct gatggcggcc gcgacgatac tgattttcgt
     2941 ttccgtcgtg caccggttcg gcaccggcgt gccgtttctg tatccctatc tgatcgccat
     3001 tcacatctcc tgggcgcagg agctgtgcat ttacatgttc atctggatgg cgaagttcgg
     3061 cgcggcctat ggggtgcgca ccggtatcca tgtcggcgtc gacgtcctga tcaaccagct
     3121 caacccgcgc tggcaaaagc gcgtcatcct gttcggtctg tgcggcggtg cgctgtttac
     3181 cttcatcgtc ggctcgatgg gtgcgaaatt cgttttcgaa ctaatgagca ccgaccaggt
     3241 ctcgcccgat ctggaattgc cgagctggct ggtctacgcc tgcattccgc tcggctccta
     3301 tctgatgtgc ttccggtttc tgcaggtgtc gtggacctat ttccggaccg gcgacctgcc
     3361 gcatcatgat gcgacgcacg tcgacggcat cgaagtcagc tcgaaggcgc cggtggcgct
     3421 gggagaagcg tgatgagcgc tgctcttatt ttcggactgc tgttcgctct gatgctgacg
     3481 gggatgccga tctcgatttc gctcggcctg acggttctga ccttcctgtt cacgatgacg
     3541 gaagtgccga tcgagagcgt cggactgaaa ttgttttccg gtctcgacaa tttcggaatc
     3601 atggcgatac cgtttttcat cctcgccggc accttcctga cccgcggtgg cgtcgcgcgc
     3661 cgcatgatcg cctttaccac ctcgctggtc ggccatttgc cgggagggct cggccttgcc
     3721 ggtgtagcgg cctgcgcgat gttcgcggcg atttcgggat ccagcgttgc caccgtggtg
     3781 gcgatcggct cgatcatgat gcctgccatg gtcgagcatg gctatccgcg ccggttcggc
     3841 gtcggcgtga tctcgacctc cggcgcgctg ggcatcctgg tgccgccgtc gatcatcctc
     3901 gtgctgtatg gcgtttccac caacacctcg atcggggcgc tgttcatggc cggcatcgtg
     3961 ccgggaatca tgctggcttc gatgcttgcg gtggtgactt ggttcatcgc ctggcgcaac
     4021 ggctacccac ggatgcaaag ggctacgttc agggaacaat ggaccgcgtt ccgtgagagc
     4081 gtctggggcc tgctgctgat cgtcatcgtg ctcggcggca tctatggcgg aatctttacg
     4141 ccgacggaag ccgccgccgt ggccgcggtc tatgccttct tcatcacggt gttcgtctac
     4201 aaggaactga aactccgcga agtctcaaaa gtgctgctgc aggcggcgag tatgagttcg
     4261 atgctgctct acatcatcac caatgcggtg ctgttctcgt tcctgatgac ccacgagcag
     4321 atcccgcagg agatggcggc ctggatcatc gacaagaatt tcagtctgtg gatgttcctg
     4381 ctggtagtca acatcctgct cctgatggcg ggcaacgtca tggacccgtc gtcgatcctg
     4441 ctgatcatgg caccgatcct gtttccgctc gcgaccaaga tcggcatcca tccggttcac
     4501 ctcggcgtcc tgatgatcgt gaataccgag gttggactgt gccatccgcc ggtcggcctc
     4561 aatctctata tcgccagcgg cattgccaaa atgggcatca gcgaaatcac catcgcggtg
     4621 ctgccctggc tctgcgcgat gctgttgttc ctggggctga ttacctacat ccccgaaata
     4681 tcgctgtggt tgccgcgcct gctcggcatg atctcatgat ctggaatctg atttgaccga
     4741 gttgaatcgg gcacgatctt tacggcattg gatcaagccg cagggcgtgc ttcgcggatc
     4801 atgctctcag agatcgaccc gccccggccg tgaccgagat cacatatccc tgcgtgaacc
     4861 ccgactagcc ttgctgcatc caatctcacg ccgagatttg ttgtgggaga gcgcgatgag
     4921 cccatccaag ggaacgttat tgggtgcctg tcttttggcc agtctgctgg cggtcgggcc
     4981 gatggctcat gcgcagcgag caaccgaagc cgccgccgtg gatcagtcga cgcaatcgct
     5041 gccgccggtc cagagatcac tgcagacggc ggcaaggccg gctaccccag ccaatcccct
     5101 gggtggcccg gagcagcgcg tcgcgctggt catcggcaat tcgaactacc aaaacgtgtc
     5161 ccagctctca aatcccgcca atgacgcgaa gtcggtggca caactcctca atgccgcggg
     5221 ctttgaggtg atttcagcga cggatctcag tcacaatgag atgattcagg tcgtgcagga
     5281 tttctccagc aagattgcgg atcgcgggcc gaacacggtg gcaatggtgt attatgccgg
     5341 ccacggcgtg caactcgcgg gcgagaatta tctcgttccc gtcgacgcaa gaatttcttc
     5401 cgagaccgat ctggtcagtg gttcggtccg gttggtcgac atgatggcga cgctggacgc
     5461 gatcccgagc cgcctgcgga tcgccatcct cgatgcctgc cgcaacaatc cgtttccctc
     5521 gctcaacgag gccggacgcg gactggcgat cgtcgatgcg ccgaacggct caatcgtggc
     5581 ctattcgact gcaccgggca gcgaagcgct cgacggcgtc ggcgaccata gtccttacac
     5641 cgctgcgttc ttgcggctgg cgcatgagaa aaacctgccg atcgaacaat tgttcaagcg
     5701 cattcgtctt gacgtgaaca attcaaccgg tgggcagcag accccctggg aaagctcgtc
     5761 gctcacaagc gatttctatt tctttggcga taccgcgctt gcagcaacga ggctgccggc
     5821 acaggagaaa gccgtctaca cggcggatga tcttccgtcg cgcgcctcgc gcgcggccta
     5881 tgactacgta ttggctgaaa attccatcga gcactatcag gaattcatcc agctttaccc
     5941 tcacgacccg ctctgctacc gcatccgcgc gctgctggac agcctcatgc aggcgaaggc
     6001 atggcataat gcggtgctga caaattcaac cgtcgcgtac caatcgtttt acgacaagtt
     6061 cgccagcagt ccctatgcgc agagtgcctt gaagttggcg cagcagccga aaatggttcc
     6121 gctctcgcag cccacgcata tcatggtgcc agcttcgctg aagttcggcg gtctcggatc
     6181 cggaaaccgt aacgcggctc ccggcaagat cgtcaatctg ccggtcggtg gtttgaacaa
     6241 atccgacgcg ctgcagaatt cgaagtttga tcgagcggta agccgcatcg accgtcggcc
     6301 tgcgaaatcg ttcgcgcaaa ttcgcgatcg tcaggggccg atgcgcatgt cgcggtccaa
     6361 cttcggcagg ccgccgagaa gcggcaacct ccgcttcgcg tcgacccagt ttcatggatc
     6421 cgggtttcga catcagttcc ggtccggcgg cggatttggc ggccatgggt tcggtggcgg
     6481 tgggttcggc ggcggcgggt ttggacgcag gtgagtgtcg tgccgtccgg caatgattgt
     6541 tgaaaaggca gaggtttctc ccaacccgcc acccgccgaa ggccgacgcg gtcctgggcg
     6601 ggtggctttg acttttctcc ccggtcgaaa ccacatcgcg gcagattaca tcttgttaaa
     6661 ccggctgatc tgtccaaact gtaagttgat tgcgggcgcg cgagcgccca tcttcggtca
     6721 accttttcaa aggaggttcg catgaggagg tttgctatcg ccggcaccgc ggcactcgcc
     6781 atcgccgctt ccaccgcggt ctatgcccag caccatcgat ggttttatga acatgtgcgg
     6841 atgagcccgg aggacagggc ggcctttacc gacgcgcgga tcgcagccgt caaggcgggg
     6901 ctgaagctta cccccgacca ggaaaaactg tggccgccgg tcgagaccgc ggtgcgggat
     6961 ttcgcaaagc tgcggatcga tcgcgccaat gcgcggatga atgccaagcc ggatgattcc
     7021 caggatgcgc agaagcagga ttctcagaat ttgcccgatc cggtcgcccg cctgcgtgag
     7081 cgcgccgatg cgatggcgac ctcagcggcc gcgatgaaac ggatcgcgga tgcggctgat
     7141 ccgctttaca agaccctgga cgacagccag aagcgccgcc tcgcgattct gacgcgtatg
     7201 gatggccggt tcggcggcgg agattggcgg caccatggct tcatgcgcgg tatggaccgt
     7261 gatgacggcg atcgggatgg cggccgaggc ggccccgagc ggctctagcc gcgtcgaccc
     7321 cctaatcgcc cgcccaatcg attgaaatgg cgcaaaggcc gccggattcc ggcggctttt
     7381 tcgttttccc tttcagcggg ttatgccggg gaaatcgact taccttgctg gacatcgggg
     7441 ggtcgctttg ctaaacgacg ccctcttgtg aaggctttcc gggccggttt ccgggcgcat
     7501 agctcagttg gtagagcaga tgactcttaa tcatcgggtc ccaggttcga gccctggtgc
     7561 gcccaccaag cttttcaagc acttagcggg cttttgcggc ttccattccg acaaactatg
     7621 gcccgtcatt ccgacaaagc ggttggtttt gttcgctcgg cacgacgctt ttgaacgccg
     7681 gcgattagct gtttgcgagt gcgcttacgt gggtagttgt cgagccgacc gatggcgtcc
     7741 tgccgcgcgc agctcggcat cggaaagatc ggaatgcacc tggaccgagc gcttccgctt
     7801 ccaatggcgc cccttgcttg cgtgcccaag ttggtggctc gaaaaaatat gcccagcggc
     7861 catttttcag cgccttgcgg cggcagtatc ggggcaactt caaagcggat ctcaacggct
     7921 tcacgcgagg gttgccagat cgagtttggg ctgattctgc ctgatggttc ggcccggcgc
     7981 aacatagtca tcgacgacag cctttgccca cactgcttca cgtgatcgcc ccacaccgcg
     8041 gtagctcgta ggcggcggcg cttcgccgcg agaaaccgcg agcactaacc caacttgcgc
     8101 agaatcgcac cctgactata agcaaatcaa tgccttagcc gaaaagttgg cactacattt
     8161 tcttgggaat tgtgatcagg cgctgtgttg gagagcaggc aaatcttgct cggtgaggcg
     8221 cattcgctt
//
LOCUS       DATNOI010000240         5450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1097862,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000240 DATNOI010000000
VERSION     DATNOI010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097862"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..330)
                     /locus_tag="VN968_09055"
                     /pseudo
     CDS             complement(1..330)
                     /locus_tag="VN968_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491272.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
     gene            complement(566..1627)
                     /locus_tag="VN968_09060"
     CDS             complement(566..1627)
                     /locus_tag="VN968_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL29421.1"
                     /translation="MPRFRSTSVSRRPLRKLLRIIVHRKPPDKLAVNPFRHYVSARAP
                     FAAKTRTGDMTENPDPETGFLEQLGQRVRTMRALHGMSRKVLAKVSGISERYIAQLES
                     GKGNVSIVLLRRVSDAMGAHLEDLIPANEPAPDWPVIRDLLRKATPNQIAQAKDILSG
                     QASGASALRRTPFAGIALIGLRGAGKSTLGKMLAKKIGWSFVELNKEIEAQNGLSVAE
                     IIALYGQEGFRRMEQAALGQLLARKQLMVLATGGGIVSEPLTFDLILSSFYTIWLKAK
                     PEEHMARVRRQGDLRPMADDRSAMAELRTILLSREPLYARASAVVDTAGLTVDAAAAR
                     LIDAVAPVLQEEARMFARG"
     gene            1626..3164
                     /locus_tag="VN968_09065"
     CDS             1626..3164
                     /locus_tag="VN968_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HXL29422.1"
                     /translation="MSGDSYNAVTWLLDRNLDEGRAKKLAFTDTVSELTYGELQKQSC
                     RLANLLRRLGVRREERVAMIMLDTTDFPIVFLGAIRAGVVPVPLNTLLTSDQYAYILA
                     DCRARVLFISEALLPVVKDMLGRMPDLDHVVVSGNDAHGYKKLSDEIPRESDSFATVA
                     THADEPAFWLYSSGSTGMPKGVRHLHSNLAATADTYAKQVLGIREDDIVLSAAKLFFA
                     YGLGNALTFPMSVGATAVLSAERPTPALMFALMNKHNPSIFYGVPTLFSAMLNDETLK
                     HERCGSRLRICTSAGEALPESVGNAWKTRFGVDILDGVGSTELLHIFLSNAPGDIKYG
                     TSGRPVPGYKVRLVNDAGSDVPDGEVGELLVDAPSAGEGYWNQRSKSRRTFEGNWTRT
                     GDKYIRDADGRYTFCGRSDDMFKVSGIWVSPFEVESALITHPAVLEAAVIPEADPEGL
                     LKPKAFVVLKADAKTGGLQEALKEHVKQKIGVWKYPRWIEVVDALPKTATGKIQRFKL
                     REHS"
     gene            3353..4171
                     /locus_tag="VN968_09070"
     CDS             3353..4171
                     /locus_tag="VN968_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL29423.1"
                     /translation="MIALTPSGTLRIGASDLEYRMVGPAADDAPTIVMLHEGLGSVGL
                     WGDFPDRLQAATGAGVFVYSRAGYGGSTPAELPRPLDYMHIEALEILPKLLDEIGFRR
                     GLLLGHSDGASIATIYAGGVQDHRIRGVAMIAPHFVVEDISVKSIAGIKQAYGTTNLR
                     SKLKRWHRDVDNAFYGWNAAWLDPKFRDWDISEYLAYIRVPVAILQGAEDEYGTIRQI
                     EIAQEECYCPVDVTIVPGAGHSPHREVPEATLNAISEFAGRILHIHEGSQGRAA"
     gene            4196..4717
                     /locus_tag="VN968_09075"
     CDS             4196..4717
                     /locus_tag="VN968_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HXL29424.1"
                     /translation="MSIPPSNAAKLPLPQWAYVPGETAEAEADHDTLWQAMALVPSRF
                     QDFVPARHPALRYGIALNDAGYFWESQEILEAVWAAAPQGGRERILLRACIQIANANL
                     RLRMQKPRAAARFFGEALGELDTLGRNATAAGDGFVDAFPTARLAALLKAKLAQPALT
                     KADWVEFGKIGRA"
     gene            4817..>5450
                     /locus_tag="VN968_09080"
     CDS             4817..>5450
                     /locus_tag="VN968_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXL29425.1"
                     /translation="MAGEDRVLANGARHIDFQTDPSRYRHWKLDVEGEVATLTMDVDE
                     NGGLFEGYQLKLNSYDLGVDIELADAVQRLRFEHPGVKVVIVRSGKNRVFCAGANIRM
                     LAGATHAHKVNFCKFTNETRNGLEDSSENSGQRFITVVNGTAAGGGYELALATDHIML
                     ADDGAAAVALPEVPLLAVLPGTGGLTRVVDKRKVRRDHADYFCTIEEGIKG"
BASE COUNT         1024 a         1692 c         1727 g         1007 t
ORIGIN      
        1 ccttgggttc agccggggtt tcaaggcact ggccggagac gtcgaacagc cagccatgga
       61 aggcgcagcg caggccgccg acctcgcatt tggccgtctc gagcacggcg gcctgcgctg
      121 cgcgcaatgg cgctcgatca ggccgtagcg tccctgctcg tcgcgaaaca acaccagatt
      181 ctcgccaagc aatttgacgg gttttaccgg gcgcgggccc tgcagctcgt cgaccagcgc
      241 cgccggctgc cagtacatcc gcatcaattt cccgcacgcg tccgcacgtc cggtccgggt
      301 catcaggtca ttctgttcct ggctcatcat ggcggtgcgt cctttcaagc gggcagcgag
      361 cccactctaa ccaaacctcg tagccccggc cagcggtctc gttccccgga tgctgcgcag
      421 cgcactcggg tccgcgctcc gcgcggcccg agcacaggct ccgcggtgcg ctgctgatcc
      481 ggggtccatg ttaacaatga gtcggtcccg gctctgcgga gcagcgcgaa gaggcgctgc
      541 accgcgtccg ggacacgaga gttgttcacc cccgcgcaaa catccgcgcc tcctcttgca
      601 gcaccggcgc aacggcgtcg atcaggcgcg cggcggcggc gtcaacggtg aggccggcgg
      661 tgtcgaccac ggcggaggcg cgggcataca gcggctcgcg gctgagcagg atggtgcgca
      721 gttcggccat cgccgagcga tcgtccgcca tcggacgcag atcgccctgg cggcgcaccc
      781 gcgccatgtg ttcctccggc ttggccttca gccagatggt gtagaacgac gacaggatca
      841 ggtcgaaggt caagggctcg gaaacgatgc cgccgccggt cgccagcacc atcagctgtt
      901 ttcgcgccag caattgtccg agcgccgcct gctccatgcg ccgaaaacct tcctggccgt
      961 agagcgcgat gatctcggcg accgagagtc cattctgcgc ctcgatctcc ttgttcagct
     1021 cgacaaaact ccagccgatc ttcttcgcca gcattttgcc gagcgtggat ttgccggcgc
     1081 cgcgcaaccc gatcagggcg atgccggcaa acggcgtgcg ccgcagcgcc gatgcgccgc
     1141 ttgcctggcc ggaaaggatg tccttggctt gcgcaatctg gttcggcgtg gccttgcgca
     1201 ggaggtcgcg aatgaccggc cagtccggtg caggctcgtt tgcgggaatg aggtcttcga
     1261 gatgcgcgcc catcgcgtca gacacccggc gcaacagcac gatcgagacg ttgcccttgc
     1321 cgctttcgag ctgggcaatg tagcgttcgg aaatgccgga gactttcgcg agcacctttc
     1381 gcgacatgcc gtgcagcgcg cgcatggtgc ggacgcgctg gccgagctgc tcgagaaagc
     1441 cggtttcggg atcgggattt tcggtcatat ccccggttcg ggttttggcc gcgaacggcg
     1501 cgcgtgcgga aacataatgc cgaaaaggat tgacagcaag cttgtccggt ggctttctat
     1561 gaactataat tcttaaaagt ttgcggaggg gacgtcgtga cacgcttgtc gaacggaagc
     1621 gcggcatgag cggcgactcc tacaatgccg tgacctggct gctcgaccgc aatctcgacg
     1681 aagggcgggc gaaaaaactc gccttcaccg acaccgtttc cgaactgacc tatggcgagt
     1741 tgcaaaagca aagctgccgc ctcgccaatc tgttgcgccg gctcggtgtc cgccgcgaag
     1801 agcgcgtcgc gatgatcatg ctcgacacaa cagattttcc gatcgtgttt ctcggcgcga
     1861 tccgcgccgg cgtggtgccg gtgccgctca acaccctgct gacctccgat cagtatgcct
     1921 acatactggc ggattgtcgc gcccgggtgc tgttcatttc cgaagcgctg ctgccggtgg
     1981 tcaaggacat gctcgggcgg atgcccgatc tcgatcatgt cgtcgtttcc ggcaatgatg
     2041 cgcacggcta caagaaactg tccgacgaga tcccgcgcga gagcgattca ttcgcaacgg
     2101 ttgcgaccca cgccgacgag ccggcgttct ggctgtattc gtcgggctcg accggcatgc
     2161 ccaagggcgt gcggcacttg cattccaatc tggcggcgac ggccgacacc tacgccaaac
     2221 aggttctcgg cattcgcgag gatgacatcg tgctgtcggc ggcaaaactg tttttcgcct
     2281 atggcctcgg caacgcgctg acgtttccga tgtcggtcgg tgccaccgcg gttctcagtg
     2341 ccgagcgtcc gacgccggcg ttgatgtttg cgctgatgaa caagcacaat cccagcattt
     2401 tctatggcgt gccgacgctg ttttccgcga tgctgaacga cgagacgctg aagcacgaac
     2461 gctgcggctc acgcttgcga atctgcacct ccgcaggcga agcgctgccg gaatcggtcg
     2521 gcaacgcgtg gaagacgcgc ttcggcgtcg atatcctcga cggcgtcggc tcgaccgagc
     2581 tgttgcatat ttttctctcc aatgcgcctg gcgatatcaa atacggcacg tcgggacgtc
     2641 cggtgccggg ctacaaggtg cggctggtga acgacgcagg tagcgatgtt cctgacggcg
     2701 aggtcggcga actcctggtc gatgcgccct ccgccggaga gggctactgg aatcagcgca
     2761 gcaaaagccg caggacattc gagggaaact ggacccgcac cggcgacaaa tacattcgcg
     2821 acgccgacgg gcgttacact ttctgcggcc gcagcgacga catgttcaag gtgtccggca
     2881 tctgggtgtc gccgttcgag gtcgagagcg cgttgatcac ccatcccgcg gtactcgaag
     2941 ccgccgtaat acccgaagcc gacccggagg gcttgctgaa gccgaaagcc tttgtggtgc
     3001 tgaaagcgga tgccaaaacc ggcggcctgc aggaagcgct gaaggagcac gtcaagcaga
     3061 agatcggcgt gtggaaatat ccgcgctgga tcgaagtcgt cgacgccttg cccaaaaccg
     3121 cgaccggaaa gatccagcgg ttcaagttgc gcgaacatag ctagccgtca tggtcgggct
     3181 tggcccggcc atccacgcct tgagtggcag cccaagaaag acgtggatgc ccgcgacccg
     3241 tctacgccga ggcttcgacg ggctttcagt gtcagtccgc cggggcttta gcgaaggcgg
     3301 gcaagcgcgg gcatgacgaa aatgagtttc aaactgcgcg ttaaagagag acatgatcgc
     3361 cctcacaccc tccggtacct tgcgcatcgg cgcttccgat ctcgaatacc gcatggttgg
     3421 tcccgcagcc gatgacgcgc cgaccatcgt catgctgcac gaaggtcttg gctcggttgg
     3481 gctgtgggga gattttccgg accggcttca ggccgccacc ggagccggtg tgttcgtcta
     3541 ttcgcgcgcg ggctatggcg gctcaacgcc ggccgagctg ccgcggccgc tcgactacat
     3601 gcacatcgag gcgctcgaga ttttgccaaa actgctcgac gaaatcggct tccgccgcgg
     3661 ccttctgctc gggcattccg acggcgcttc gatagcgacg atctacgccg gcggtgttca
     3721 ggatcaccgc atccgcggcg tagcgatgat cgcgccccat ttcgtcgtcg aagatatttc
     3781 ggtgaagtcg atcgccggga tcaagcaagc ctatgggacc accaatctcc ggtcgaagct
     3841 gaagcgctgg catcgcgacg tcgacaacgc gttctacggg tggaacgcgg catggctcga
     3901 cccaaagttt cgcgactggg atatttcgga atatctcgcc tacatacggg tgccggttgc
     3961 aatcctgcag ggcgccgagg acgagtatgg gacaatccgc cagatcgaga tcgcccaaga
     4021 agaatgctat tgtccggtcg atgtgacgat cgtccctggt gccggacatt cgccgcatcg
     4081 tgaagtgccg gaggcgacgc tgaacgcgat ttcggaattt gcaggacgca ttttgcacat
     4141 ccacgagggc tcgcaggggc gggccgccta acgcattggt cggggccatg cgcgcatgag
     4201 cattcctccc tcaaatgccg ccaagcttcc gctgccccag tgggcctatg ttcccggcga
     4261 aactgccgag gccgaagccg atcacgatac gttatggcag gcgatggccc tggtgccgtc
     4321 gcgattccag gacttcgttc cggcgcggca tccggcgctg cgctacggga ttgccctcaa
     4381 cgacgccgga tatttctggg aatcgcagga aatcctggaa gcagtttggg cggcggcgcc
     4441 gcaaggcgga cgcgagcgaa tcctgctgcg ggcctgcatc cagatcgcca acgccaatct
     4501 caggctgcgg atgcaaaagc cgcgtgcggc ggctcggttt tttggtgagg cgctgggcga
     4561 actcgataca cttggacgca atgcgacggc ggccggcgac ggattcgtcg atgcttttcc
     4621 gacggccaga ctcgcggcac tcctgaaggc caaactggcg cagccggcgc taaccaaggc
     4681 ggattgggtg gagtttggca aaatcggccg agcatgaaac aaaatgcata tttcggccca
     4741 atagccccta gacctcgccc aaaacatgca ttattatgca tgttaacgga tagcctaaaa
     4801 acaggtgagg gtgcccatgg ccggcgaaga tcgcgtcctc gctaatggcg caaggcatat
     4861 cgattttcag accgatccgt cgcgctaccg gcattggaag ctggacgtcg agggcgaggt
     4921 cgcgacgctc accatggatg tcgatgagaa tggcgggctg ttcgaaggct atcagctcaa
     4981 gctgaattcc tacgatcttg gcgtcgacat cgaactggcg gacgcggtgc agcggctccg
     5041 cttcgagcat cccggcgtga aggtcgtgat cgtgcgctcc ggcaaaaacc gcgtgttctg
     5101 cgccggcgcc aacatccgca tgctggcggg ggccacccac gcccacaagg tcaacttctg
     5161 taaattcacc aacgagaccc gcaacggact ggaagactcg tccgagaatt ccgggcagcg
     5221 tttcatcacg gtcgtgaacg gcactgcggc cggcggcggc tatgagctgg cgctcgcaac
     5281 cgatcacatc atgctggccg acgatggtgc cgcggcagtg gcgctaccgg aagtgccgct
     5341 gcttgcggtg ttgccgggca ccggcggact gacgcgcgtg gtcgacaagc gcaaggtgcg
     5401 ccgcgatcac gccgattatt tctgcaccat cgaggaaggc atcaagggca
//
LOCUS       DATNOI010000241        10830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_38535,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000241 DATNOI010000000
VERSION     DATNOI010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38535"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4363
                     /locus_tag="VN968_09085"
     CDS             <1..4363
                     /locus_tag="VN968_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HXL29426.1"
                     /translation="GKAYVLPRTGQRGIPLVSVNTPSVSVNVFRIGDRNLINTVIDSD
                     FQRSLSKYELSRLGNERGVKVWSGELATATTLNQDVTTAFPVDQALGDLQPGVYVMTA
                     AAKGPGSDDDGSLATQWFIVSDLGLTAFSGNDGIHVFVHSLASTDAVPKADVRLVARN
                     NEILANRKTDESGHVLFEAGLARGEGGLSPAMLTVTSEKADYAFLSLKTDAFDLSDRG
                     VAGRAVPSGADAFVYAERGVYRSNETVYLTALLRDGQGNAVTGGPLTLVIERPDGVEF
                     RRAVLTDQGAGGRSMALALNSAVPTGTWRVRAFTDPKGSSVGETTFMVEDYVPERIEF
                     DLSAKDKQIKAEAPVELKVDGHFLYGAPASGLQLEGDMLVAPASARPGFAGYQFGVAD
                     EQTASNERTPIENLPEADANGVATFPVSLAKPPSSTRPQEAQIFIRMAEAGGRAVERK
                     LVLPVAPATAMIGVKPLFGDKSVAEGDKAGFDVVFVAPDGKQLARDGLRYELLRIASR
                     FQWYRQNSSWDYEPVKSTTRVADGDLTVAADKPSRITLSPQPGRYRLDVKSTDTDGPL
                     TSVQFDVGWYSDGSADTPDLLETSIDKPEYASGDTMVVSVNARSAGKLTINVLGDRLL
                     TTQSTDIKEGTAQVKIPVGKDWGTGAYVVATLRRPLDAAALRMPGRSIGLKWFGIGKK
                     TRTLQVNLSPPALVRPGATLKLPVKLGGLSPGEDAKIVVAAVDVGILNLTNYKPPAPD
                     DYYLGQRRMSSEIRDLYGQLIDGMQGTRGQIKTGGDAAGAELQGSPPTQKPLALYSGI
                     VTVAADGSAEISFDIPEFAGTARVMAVAWTATKLGRATTDVTVRDPVVLTATLPRFLL
                     NGDHGTMSFDLDNVEGTPGDYTINVKTSGPVKVTGNSDTTVKLAAGQRTSMSLALDAG
                     GAGMANLDVDIKGPNGLTLARHYALDVKPATQILARRSIRTLAKGESLTLTSDMFTDL
                     VQGTGGVSLSVSLSTALDAATILKALDRYPHGCSEQITSRAMPLLYVNDLAAGAHLAM
                     DTAVDQRIKDAIDRLLARQGSNGSFGLWSAGGDDAWLDAYVTDFLTRAREKGFAVPDV
                     LFRNALDRIRNSVVNANEPEKGGGRDLAYGLYVLARNGAAPIGDLRYLADTKLDNLAT
                     PIAKSQLAAALALVGDKGRAERVYAAALESLAPKPVLEFGRTDYGSALRDAAALVSLA
                     SEGNAPRATLTQAVARVEVARGLTPYTSTQENAWLVLASRALAKETMTLDVDGAAVKT
                     ALYRSYKAAEMEGKPIKITNTGDAAVQAVVSVSGAPVTPEPAASNGFKIERSYFTLDG
                     KPADVAKARQNDRFAVVLKITEPKPEHGHIMVSDYLPAGLEIDNPHLVSSGDTGTLDW
                     IEDGEKPENTEFRDDRFTAAIDRAANDKAVFTVAYVARAVSPGRYVLPQAYVEDMYNP
                     SRYGRSGTGAVEVRPAK"
     gene            4480..6594
                     /gene="pbpC"
                     /locus_tag="VN968_09090"
     CDS             4480..6594
                     /gene="pbpC"
                     /locus_tag="VN968_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HXL29427.1"
                     /translation="MDGRDKPGHDGIGEGRARRVFIGIAFAILAIVGVFAAWVVSLGP
                     LPLAQARQVSTTIVDRNGKLLRAYAMADGRWRLPVDARHNVDPGYLKLLLAYEDRRFY
                     SHAGVDPLALGRAAFQLVTRGHIVSGGSTITMQLARLMEPRRERSVHAKLRQMVRALE
                     IERQLTKAQILDLYLALAPFGGNLEGVRAASIAYFGKEPKRLSLAESALLVALPQSPE
                     NRRLDRYPNVARAARDRVLNRVVEEGHVTAEDAAQAKSVGVSRLRKPMPILAPHSADQ
                     AMATVKDADVIALTLDSSLQKVLEALARDRATALGQNISIAIIAVDNESGDVLARVGS
                     PDYFDDRRAGQVDMTRAVRSPGSTLKPFIYGLAFEDGFVHPESLIDDRPIRFGSYAPE
                     NFDMTFQGTVPVRKALQLSLNVPAIALLDRVGASRLSSRLKQAGVNLVLPKDEAPGLA
                     MGLGGVGVTLQDLAQLYSGLARLGSTRPLREIVKATDAPRDSLRLMDQAAAWQVGNVL
                     IGTPPPENGVHNRIAFKTGTSYGYRDAWSVGFDGRITIGVWVGRPDGAPVPGLVGRTA
                     AAPILFDAFARTGKLPAALPKPPKGTLVASNAKLPLPLRRFRPIGELVWTGGEQAPHI
                     QFPLNGSRIDLDRSGGTELAAMPVKVVGGVLPLTMLMNGVSVGEIDSRRQRLVDPPGP
                     GFARLTVIDAMGAADTVVIRIQ"
     gene            6646..8388
                     /locus_tag="VN968_09095"
     CDS             6646..8388
                     /locus_tag="VN968_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXL29428.1"
                     /translation="MVETYARPRFGEPRQPKNRVNPGSRLVGVLDFATASHVRAIAFL
                     LLCGLLLFLPGFFNIPPIDRDEARFAQATKQMVESGDFVDIRFQDEVRYKKPVGVYWL
                     QAAAVETASALGLPRAQVRVWLYRVPSLIGAIGAVLLTYWTALAFVTRRGAVLAGLIL
                     GSSVLLGVEARLAKTDAMLLLTVVAAMGALARVYLSWQRGEDPAHPPWTWPVVFWTAL
                     AAGILLKGPLILMFVVLTIATLAILDRSATWLWRLRPVWGLMWLLVLVLPWFIAIFWR
                     AGDAFFADSIGGDMLSKLAAQESHGAPPGVYLLLFWVTFWPGAPLAAMATPAVWRARR
                     EPGAQFLLAWLVPSWIVFELVLTKLPHYVLPLYPAIAILTIGALERRVLSRSWLMRGA
                     AWWFVIPAGASVIAVIGAITLTRQPVFLAWPFVAAALIFGLFAWWLYDDNRAERSLLN
                     AVVAAMFLAVAIYGVIVPALTPLFPSSEIARALRNVVCVGPKAAAAGFHEPSLVFMTG
                     TSTVLTDGSGAADFLGQGSCRFALVEARSERGFAQRAEAIGLRYNVAARIDGYNISQG
                     RAISIAIFRSEGTE"
     gene            8389..9234
                     /locus_tag="VN968_09100"
     CDS             8389..9234
                     /locus_tag="VN968_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXL29429.1"
                     /translation="MSAQAGIGGQSNYFSQVLFLARTSATQLVRPPSHSRRAEAARRS
                     ARRGLLLVAILGAVIIALMVALDVAEIGLMPPRGTASLWPVRILTDFGKSAYVLWLLA
                     IMLFAVAVIFPRLRATSRSLLLSFGTRLQFLFFAVLVPVLAGELIKWIVGRGRPFVGG
                     KADAFNFAPFVGTEAYASFPSSHAIVGFALAFAVTALWPRTWAAMMVYAILIAASRLV
                     LLAHHPSDVVAGALVGIVGAMFVRYWFAARRLGFAIRHDGTIVPLAGPSPAHLKRVAR
                     DAFAP"
     gene            9320..10072
                     /locus_tag="VN968_09105"
     CDS             9320..10072
                     /locus_tag="VN968_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXL29430.1"
                     /translation="MPSSDAATVDVSIVVPVRNEAENIGPLIAEIAAALGGRWQYEII
                     YVNDGSTDTTAERIAAIMKQRENLRQIRHATSSGQSAAVRSGVRAARGAIVATLDGDG
                     QNNPAFLPDLISAIEKGGECVGLAAGQRVGRKDTGFKKIQSRIANGVRNGILRDGTRD
                     TGCGLKAFRREVFLMLPYFDGLHRFLPALVRREGYEIAYVEVTDRPRRSGVSNYGFFD
                     RLWIGIMDLAGVWWLIRRKKSMPVVTEVMSND"
     gene            10065..10385
                     /locus_tag="VN968_09110"
     CDS             10065..10385
                     /locus_tag="VN968_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HXL29431.1"
                     /translation="MTDMLNTFVTYLHDVFVIKFDGWVILGFVAQGFFTMRFVVQWIA
                     SERARKSVIPVAFWFFSIGGGALLLVYALYRRDPVFIAGQALGLVVYVRNLYFIIVNG
                     RQAT"
     gene            complement(10387..>10830)
                     /locus_tag="VN968_09115"
     CDS             complement(10387..>10830)
                     /locus_tag="VN968_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HXL29432.1"
                     /translation="EHMGDIIDKNLSELATKKIKRRFQFSLEGAEELSAFHKRIMDSL
                     RIAFGVFMSGDVNEARKLLAEKALLRNTELAATERHLDRLREGRPETIETTSLHLDVL
                     RDLRRIHSHICSVAYPVLDAAGELAAYRSTESDVAALPAAHPLPR"
BASE COUNT         1784 a         3421 c         3580 g         2045 t
ORIGIN      
        1 cggaaaggcc tacgtgctgc cgcgcaccgg ccagcgcggc attccgctgg tcagcgtcaa
       61 cacgccctcg gtatcggtca atgtctttcg gatcggcgat cgcaacctga tcaatacggt
      121 gatcgacagc gactttcagc gtagcttgag caagtacgag ctttccagac tgggcaatga
      181 gcgcggcgta aaagtctggt cgggcgaact tgccaccgcg accacgctca atcaggacgt
      241 caccacggcg tttccggtcg accaggcact cggcgacctg cagccgggcg tctatgtcat
      301 gaccgccgcg gccaaaggcc ccggcagcga cgacgacggc tcgctggcga cgcagtggtt
      361 catcgtatcc gatctgggcc tgaccgcgtt ctccggtaat gacggcatcc atgttttcgt
      421 ccattcgctg gcctcgaccg atgcggttcc caaggcggac gtgcggctgg tggcgcgcaa
      481 caacgagatt ctggccaacc gcaagaccga cgagtcaggt cacgtgctgt tcgaggcggg
      541 cctggcgcgc ggcgagggcg gtctgtcgcc ggcgatgctg acggtgacca gcgagaaagc
      601 cgactacgcg ttcctcagcc tgaaaaccga cgccttcgac ctctcggatc gcggcgttgc
      661 cggccgcgct gtgccatcag gcgccgatgc cttcgtctat gccgaacgcg gcgtctatcg
      721 ctccaacgaa accgtgtacc tcacagccct tctgcgcgac ggccagggca atgccgtgac
      781 cggcgggccg ctgacgctcg tgatcgagcg tcccgacggc gtcgaattcc gccgcgccgt
      841 gctgacagac cagggggcgg gcggacgcag catggcgctg gcgctcaact ccgccgtccc
      901 caccgggacc tggcgggtgc gggcgttcac cgatcccaag ggctcgtcgg tgggcgaaac
      961 caccttcatg gtcgaggact acgtccccga acgtatcgaa ttcgacctct cggccaagga
     1021 taaacagatc aaagcggaag ctccggtaga gcttaaggtc gacggccatt tcctctatgg
     1081 cgcgccagcc tccggtctgc agctcgaagg cgacatgctg gtggcgccgg cttccgcgcg
     1141 cccggggttt gccggctatc agttcggcgt cgccgacgag cagactgcca gcaacgaacg
     1201 cacgccaatc gaaaacctgc ccgaggccga cgccaacggc gtcgcgacct tcccggtcag
     1261 tctggcaaag ccgccgtcat cgacgcggcc gcaggaagcc cagatcttca ttcgcatggc
     1321 cgaagccggc ggtcgcgccg tcgagcgcaa gctggtgctg ccggtcgcac cggcgacggc
     1381 gatgatcggc gtcaagccgc tattcggcga caagagtgtg gccgagggcg acaaggccgg
     1441 tttcgacgtc gtgttcgtcg ctcccgacgg caagcaactg gcgcgcgatg gcctgcgcta
     1501 cgagctgttg aggatcgcat cccgctttca atggtatcgg caaaattcgt cctgggacta
     1561 cgagccggtt aaatcgacca cgcgggtcgc cgatggcgac cttaccgtcg ccgccgacaa
     1621 gccgtcgcgg atcacgctgt cgccgcagcc cggccgctat cgtctcgatg tcaaatcaac
     1681 cgacaccgat gggccgctga cctcggtgca gttcgacgtc ggctggtatt ccgacggcag
     1741 cgccgacacg cccgatctgt tggaaacctc gatcgacaaa ccggaatatg catccggcga
     1801 caccatggtg gtctcggtca acgcgcgttc ggcgggaaaa ctcaccatca atgtgctggg
     1861 cgaccggttg ttgacgacgc agagcaccga catcaaggaa ggcaccgcgc aggttaaaat
     1921 tcccgtcggc aaggattggg gcaccggcgc ctatgtggtg gcgacgctgc gccgtccgct
     1981 cgatgccgcg gcactgcgga tgcccggtcg gtccatcggc ctgaaatggt tcggcatcgg
     2041 caagaagacc cgcacgctcc aggtcaatct ctcgccgccg gccctggtgc gtcctggcgc
     2101 tactttaaaa ctgccggtta agctgggcgg gctaagccca ggcgaagacg ccaaaatcgt
     2161 cgttgcagcg gtcgacgtcg gcattctcaa tttgacgaat tacaagccgc cggcgccgga
     2221 cgattactat ctcggccagc gccgcatgag ctcggaaatc cgcgacctct atgggcaatt
     2281 gatcgacggc atgcagggga cccgcggcca gatcaagacg ggcggcgacg ccgccggcgc
     2341 cgagctgcag ggcagtccgc cgacgcaaaa gccgctggcg ctgtattccg gcattgtcac
     2401 ggtggccgcc gacggttcgg ccgaaatcag cttcgatatt cccgagtttg ccggcaccgc
     2461 gcgggtgatg gcggtggctt ggaccgcgac caagcttggg cgagccacca ccgatgtcac
     2521 ggtgcgcgat cccgtggtgc tgacggcgac gttgccgcgt ttcctgctca acggcgatca
     2581 cggcaccatg agttttgatc tcgacaatgt cgagggcacg ccgggcgatt acaccatcaa
     2641 cgtcaagacg tcggggcccg tgaaggtcac tggcaattcc gacaccacgg taaaactcgc
     2701 ggccgggcag cgaacctcga tgtcgctggc gctcgatgcc ggcggcgccg gaatggcgaa
     2761 cctcgatgtc gacatcaagg gaccgaacgg gctgacgctg gcgcggcatt atgcgctcga
     2821 cgtcaagccg gcgacgcaga ttctggcgcg ccgctcgatc cgcactttgg ccaagggtga
     2881 gagcctgacg ctgacgtccg acatgtttac ggatctggtg cagggcaccg gcggcgtctc
     2941 gctgtcggtc agcctgtcga cggcgctcga tgccgcgacc atcctgaagg cgctggatcg
     3001 ttatccgcat gggtgctcgg agcagatcac cagccgcgcg atgccgctgt tgtacgtcaa
     3061 tgatctggcg gcgggcgcgc atcttgcgat ggacacggcg gtcgatcagc gcatcaagga
     3121 tgcgatcgat cggctgctgg cgcggcaagg ctccaacggg tcgttcgggc tatggtcggc
     3181 cggcggcgac gacgcctggc tcgacgccta tgtcacggat tttctcaccc gcgcccgcga
     3241 aaagggattt gcggtgccgg acgtgttgtt cagaaacgcg ctcgatcgca tccgcaactc
     3301 ggtcgtcaac gccaatgagc ccgaaaaggg cggcgggcgc gatctcgctt acggcctcta
     3361 tgtgctcgcc cgcaatggcg cagcaccgat cggcgatctg cgctaccttg ccgacaccaa
     3421 gctcgacaat ctcgccaccc cgattgccaa atcgcagttg gcggcggcgc tggcgctggt
     3481 tggcgacaag ggcagggcgg agcgggtcta tgccgcagcg ctcgaaagcc tcgcgccaaa
     3541 gccggtgctg gagttcggcc gaaccgacta cggcagcgcg ttgcgcgacg ccgcggcatt
     3601 ggtttcgttg gccagcgaag gcaatgcccc gcgggcaacg ctgacgcagg ccgtcgcacg
     3661 cgtggaagtg gcgcgcgggc tgacgcctta cacctcgacc caggaaaatg cctggctggt
     3721 attggcgtcg cgggcgctgg ccaaggaaac aatgaccctc gatgtcgatg gcgcagcggt
     3781 caagaccgcg ctgtatcgca gctacaaggc cgccgagatg gagggcaagc cgatcaagat
     3841 caccaacacc ggcgatgcgg cggtgcaggc ggtggtttcg gtgagcggcg cgccggtaac
     3901 cccggagccg gcggcgtcga atggtttcaa gatcgagcgc agttatttta ccctcgacgg
     3961 caagccggcc gacgtcgcca aggccaggca gaatgaccgt ttcgccgtcg tgctcaagat
     4021 caccgagccc aagccggaac acggtcacat catggtgtcg gattatctcc ccgcaggtct
     4081 cgagatcgac aacccgcatc tggtctcgtc cggcgacacc ggcacgctgg actggatcga
     4141 ggacggcgaa aagccggaaa ataccgaatt ccgcgacgac cgcttcaccg cggccatcga
     4201 ccgcgctgcg aacgacaagg cggtgttcac ggtggcctat gtggcgcgcg cggtatcgcc
     4261 gggcagatac gtgctgccgc aagcctatgt cgaggacatg tacaatccct cgcgctatgg
     4321 ccgctccggc accggcgcgg tcgaggtgcg cccggcgaaa tgagcgacgc gggctctcac
     4381 gtttcctcgt cgtcatgccc gggcaaaggg cgcgaagcgc cgtcttcgcg ccaggtgtcc
     4441 cgggcatcca cggcttttgc tgccgcggac agggaagacg tggatggccg ggacaagccc
     4501 ggccatgacg gtattggtga gggacgcgcg cgccgcgtct ttatcggcat tgcctttgcc
     4561 attttggcca tcgtcggcgt cttcgcggcg tgggttgtct cgctgggtcc attgccgctc
     4621 gcgcaggccc ggcaagtctc caccaccatc gtcgaccgca atggcaaatt gttgcgtgcc
     4681 tacgcaatgg ccgacggccg ttggcggctg ccggtcgatg ccaggcacaa tgtcgatccg
     4741 ggctacctca aattgctgct ggcgtatgag gaccggcgtt tttattccca tgcgggcgtc
     4801 gatcccctgg cattgggccg cgccgcgttt cagctcgtta cccgcggtca cattgtttcc
     4861 ggcggctcga ccatcacgat gcaattggct cgattgatgg agccgcggcg cgaacgctcg
     4921 gttcacgcca agctgcgcca gatggtccgc gcactcgaaa ttgagcggca actgacaaaa
     4981 gctcaaattc tcgacctcta tctggcgctg gcgccgttcg gcggcaatct cgagggcgtg
     5041 cgcgcggcct cgatcgctta tttcggcaag gagccgaagc ggttgtcgct ggcggaatcg
     5101 gcgctgctgg tggcgttgcc gcaatcgccg gaaaaccgca ggcttgatcg ctaccccaac
     5161 gtcgctcgcg ccgcgcgtga ccgggttctc aatcgcgtgg tcgaagaggg tcatgtgacg
     5221 gccgaggatg cggctcaggc aaaatccgtt ggggtgtcgc ggctgcgcaa gccgatgcca
     5281 atccttgcgc cgcattctgc cgatcaggcg atggccaccg tgaaagatgc cgatgtcatc
     5341 gcgctgacgc tggattccag tctgcaaaag gttctcgaag cgctggcgcg cgaccgcgcg
     5401 accgcgttgg gccagaatat ttccattgcc atcatcgcgg tcgacaacga gagcggcgac
     5461 gtgctggcgc gggtcggttc gcctgactat ttcgatgacc ggcgcgccgg gcaggtcgat
     5521 atgacgcgcg cggtgcgttc gccgggctcg acgctcaagc cattcatcta tggcctcgcg
     5581 ttcgaggatg gctttgttca tccggaaagc ctgatcgacg accggccgat ccgcttcggc
     5641 tcttacgcgc cggagaattt cgacatgacg tttcagggaa cggtgccggt gcgcaaggcg
     5701 ctgcaactgt cgctgaacgt gccggcaatc gctttgctcg accgcgtcgg tgctagccgt
     5761 ctgtcgtcac gcttgaagca ggcgggcgtc aatctggtgc tgccgaagga cgaagcgccg
     5821 ggcctcgcga tggggctcgg tggcgtcggc gtcacgctgc aggacctggc gcagctctat
     5881 tcgggattgg cccggctggg cagcacccgg ccgctgcgcg agatcgtgaa agcgaccgac
     5941 gccccgcgcg attccctgcg actgatggat caggccgcag cctggcaggt cggcaacgtg
     6001 ctgatcggca cgccgccgcc ggaaaatggc gtgcacaacc ggatcgcgtt caagaccgga
     6061 accagctatg ggtatcggga tgcatggtcg gtcggcttcg acggccgcat caccataggt
     6121 gtctgggtcg ggcgtcccga tggcgcgccg gttcccggtc ttgtcggccg caccgccgcg
     6181 gcgccgattt tgttcgatgc cttcgcccgc accggaaaat tgcccgcagc cctgccaaaa
     6241 ccgcccaaag gtacgctggt agcgagcaac gccaaactgc cgttgccgct gcggcggttc
     6301 cggccgatcg gggaactggt ctggaccggc ggcgagcagg ccccgcacat ccagtttccg
     6361 ttgaatggct cgcggatcga tttggatcgc tcagggggta cggaacttgc cgcgatgccg
     6421 gtcaaagtcg tcggaggcgt gctcccgctg acaatgctca tgaacggtgt atcggtgggc
     6481 gaaatcgaca gccggcggca gcgtctggtc gatccacccg gcccgggttt cgccagattg
     6541 accgtgatcg acgccatggg tgccgcggac acagttgtca tccgaatcca atagaatctt
     6601 gctaaccggt tgcccccgat agcagtttcg gcgtatgcgg aaccgatggt cgagacctat
     6661 gcccgccccc gatttggaga gccgcgccag cctaagaacc gggtgaaccc gggcagcagg
     6721 cttgtcggcg tgcttgattt cgccaccgcc agtcatgtcc gcgcgattgc gttcctgctg
     6781 ctgtgcggtc tgctgctgtt tctgcccgga tttttcaaca ttccgccgat cgaccgcgat
     6841 gaggcgcggt tcgcgcaggc gaccaagcaa atggtcgaga gcggggactt cgtcgacatc
     6901 cgtttccagg acgaagtgcg ctacaagaaa ccggtcggcg tgtactggct gcaggcagcg
     6961 gcggtcgaaa ctgcgtccgc gctaggcttg ccgcgggcgc aggtgcgcgt ctggctctac
     7021 cgcgtgccgt ccctgatcgg cgcgatcggc gccgtgctgc tgacctactg gacggcgctc
     7081 gcattcgtga cacggcgcgg agccgtcctg gccgggctga tcctgggcag ttcggtgctg
     7141 ctcggcgtcg aagcgcggct ggcgaaaacc gatgcgatgc tgctgctcac cgtcgttgcc
     7201 gcgatggggg cgctggcgcg ggtgtatttg tcatggcaac ggggcgagga tcccgcgcat
     7261 ccgccgtgga catggccggt ggttttctgg acggcgctgg ccgccggaat cctgctcaag
     7321 ggaccgttga tcctgatgtt cgtcgtgctg acgatcgcca cgttggcgat cctcgatcgc
     7381 tcggcgacat ggctgtggcg gctgcggccg gtatggggcc tgatgtggct gctggttctg
     7441 gtattgccct ggttcatcgc catcttctgg cgcgccggcg acgcgttttt cgcggattcg
     7501 atcggcggcg acatgttgag caagctcgcc gcgcaggaat cccacggcgc accgccggga
     7561 gtttatctgc tgctgttctg ggtgacgttc tggccgggag cgccattggc ggccatggca
     7621 acgcccgcgg tatggcgggc gaggcgggag cccggcgcgc agtttctgct ggcgtggctg
     7681 gttccttcct ggattgtgtt cgaactggtg ctgaccaagc tgccgcatta tgtgctgccg
     7741 ctgtatccgg cgatcgcgat cctgaccatc ggcgcactgg aacgccgcgt gctgtcgcga
     7801 tcgtggttga tgcgcggcgc ggcctggtgg ttcgtgattc ccgccggggc ttcggtcatc
     7861 gccgtgatcg gcgccatcac gctgacccgg cagccggtgt ttctggcctg gccgttcgtg
     7921 gccgcggcgc tgatcttcgg gctgttcgcg tggtggcttt acgacgacaa tcgcgccgag
     7981 cgctcgctgc tgaatgccgt ggtcgccgcg atgttccttg ccgtggccat ctacggcgtc
     8041 atcgtgccgg cgctgacgcc gctgttcccg agttctgaga tcgcgcgcgc gctgcgcaac
     8101 gtcgtctgcg tcgggccgaa ggccgctgcg gctggttttc acgagcccag tctcgtcttc
     8161 atgaccggca cctcgacggt gctgacggat ggatcgggtg ctgcggactt tctggggcag
     8221 ggcagctgca ggttcgcgct ggtggaagcc cgttcggaac gcggctttgc ccagcgcgcc
     8281 gaggccatcg ggctgcgcta caatgtcgcc gcccgcatcg acggctataa tatttcacag
     8341 ggccgggcga tttcgatcgc catcttccgc tccgagggca cggagtagat gtccgctcag
     8401 gccggcattg gcggacagtc gaattatttt tcgcaggtgc tcttcctggc gaggacgtcg
     8461 gcgacgcaat tggtgcggcc gccgtcgcat tcgcggcgcg cagaagccgc gcgaagatcg
     8521 gcgcgccgcg ggttgctgct ggtcgctatc ctcggtgccg tcatcatcgc gctgatggtt
     8581 gcgctcgacg tcgccgaaat cggcctgatg ccgccgcgcg ggaccgccag cctttggccc
     8641 gttcgcatcc tgaccgactt tggaaaatcg gcctatgtgc tgtggttgct ggccatcatg
     8701 ttgttcgccg tcgcggtcat tttcccgcga ttgcgggcga cttcacgatc gctgctgctc
     8761 agttttggca cgcgtctgca gtttctgttt tttgccgtgc tggtgccggt gctcgccggc
     8821 gaactcatca aatggatcgt cgggcgcggc cggccgtttg tcggcggcaa ggccgatgct
     8881 ttcaatttcg cccccttcgt cggaaccgaa gcctatgcca gctttccgtc gagccacgcc
     8941 atcgtcggct ttgcgctggc gttcgcggta accgcgcttt ggccgcggac gtgggccgcg
     9001 atgatggtgt atgccattct gatcgccgcc agccgcctcg tgctgctggc ccatcatccg
     9061 agcgacgtgg tcgcgggcgc gttggtcggc atcgtcgggg cgatgttcgt gcggtactgg
     9121 ttcgccgcgc gccgcctcgg gtttgcgatc cgccacgacg gcacgatcgt gcccctcgct
     9181 ggaccctcgc cagcgcacct caaaagggtt gcccgcgacg ctttcgcccc ataagaagcg
     9241 gacgccgcaa gcgccggacg caagcgtcac cggcggcggc ccaactcaag ccagccctgc
     9301 tcaagcgacg agtgttgatt tgccatcttc tgacgcagct acggtcgatg tttccatcgt
     9361 tgtgcccgta cgcaacgaag ccgagaacat cggtccgctg atcgccgaga tcgccgcggc
     9421 gctcggcggt cgatggcagt atgaaatcat ctacgtcaat gacggctcga ccgacacgac
     9481 cgccgagcgg attgccgcga tcatgaagca gcgtgaaaat ctgcggcaga tccgtcacgc
     9541 cacgtcgtca ggccagtcgg cggcggtgcg cagcggggtg cgcgcggcgc gcggcgccat
     9601 tgtcgcgacc ctcgacggcg acggacaaaa caacccggca ttcctgccgg acctgatttc
     9661 ggcgatcgag aagggcggcg aatgcgtcgg tcttgccgcc ggccagcgcg tcggacgcaa
     9721 ggacaccggc ttcaagaaaa tccagtcgcg catcgccaat ggggtgcgca acggaatcct
     9781 gcgcgatggc acccgcgata ccggctgcgg cctgaaagcg ttccggcgcg aggtattcct
     9841 gatgctgcct tacttcgatg ggctgcatcg ttttctgccg gcgctggtgc gccgcgaagg
     9901 ctacgagatc gcctatgtcg aagtgaccga tcggccgcgc cgctcgggcg tttcaaacta
     9961 tggatttttc gatcggctat ggatcgggat catggatctc gccggggtgt ggtggctgat
    10021 ccgccgcaag aagtcgatgc cagttgtgac ggaggtaatg tccaatgact gacatgctca
    10081 acacattcgt gacctacctg cacgatgtct tcgtcatcaa attcgacggc tgggtcattc
    10141 tggggttcgt ggcgcaagga tttttcacca tgcgctttgt ggtgcaatgg atcgcgtcgg
    10201 agcgcgcgcg caagagcgtg atcccggtgg cgttctggtt cttctcgatc ggcggtggcg
    10261 cgctgctgct ggtgtatgcg ctgtatcgca gagacccggt attcatcgca ggccaggcgc
    10321 tcgggctcgt ggtctacgtc cgcaatctgt attttattat cgtcaacgga cggcaggcga
    10381 catgagctat cgcggcaggg gatgcgccgc aggcagcgcc gccacatcgc tttcggtgct
    10441 tcgataggcg gccagctcgc cggcggcgtc gagcacggga taagccaccg agcagatgtg
    10501 cgagtggatg cgccgcaagt cacgcagcac gtcgagatgc agcgaagtgg tctcgatcgt
    10561 ctcgggtcgg ccttcgcgca gacggtccag atgccgctcg gtggcggcaa gctcggtatt
    10621 gcgcaacagt gccttctccg cgagcagctt gcgcgcttca ttgacatcgc ccgacatgaa
    10681 gacgccgaac gcgatccgca gcgagtccat gattcgcttg tggaatgccg acagttcttc
    10741 cgccccctcg agcgaaaact ggaacctgcg cttgatcttc ttggtcgcga gctcgctcag
    10801 attcttgtca atgatgtcgc ccatatgctc
//
LOCUS       DATNOI010000242         6772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_998604,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000242 DATNOI010000000
VERSION     DATNOI010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6772
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998604"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VN968_09120"
     CDS             <1..195
                     /locus_tag="VN968_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29433.1"
                     /translation="ALITSSISAIPGALTARVRARIAGTAGIGRQWVRSEHRKNGKRF
                     PVFLASHQRFVVGGVAVQMS"
     gene            complement(290..1054)
                     /locus_tag="VN968_09125"
     CDS             complement(290..1054)
                     /locus_tag="VN968_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXL29434.1"
                     /translation="MKKFLLGAVGLVALSFAAPASAADLAARPYTKAPAMIAAVYDWS
                     GFYVGANGGWGTSHNCYTNTAIGGVAIPAAAEGCHDATGGTAGGQLGYRWQTGGFVYG
                     LEAQGNWADLRGSNPSLAAPGATNRTRLDAFGLFTGQIGFAWNNALLYAKGGAAVTDN
                     KYEGLLTGTGTVLDRSTSTRWGATVGAGVEYGFASNWSAAVEYDHMFIGTQSNTFTSV
                     FPVAGVNTRTDSIRQDADTVTARVNYRWGGPVVGKY"
     gene            1555..2046
                     /locus_tag="VN968_09130"
     CDS             1555..2046
                     /locus_tag="VN968_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXL29435.1"
                     /translation="MAGSVNKVILIGNLGADPEIKRTQDGRPIANLRIATSDTWRDKN
                     TGERKEKTEWHRVVIFSEGLCKIAEQYLKKGAKVYIEGQLQTRKWTDQSGVEKYSTEV
                     VLQNFNSTLTMLDGRSGGGGGNFGSDDSGGDFGSSGPSTAAPRRAVAAGARNSDMDDD
                     IPF"
     gene            2238..2636
                     /locus_tag="VN968_09135"
     CDS             2238..2636
                     /locus_tag="VN968_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HXL29436.1"
                     /translation="MSLAPLLDAAPAIPLHAFAAMTAFVLGVVQFAAPKGTLPHRTTG
                     WIWVCLMAAVAVSSFWIHQIRLVGPWSPIHLLSIFTLVVLPLGVWMAHRHRVADHRRI
                     MIFIFSGALVIAGLFTLLPGRIMHSVVFGH"
     gene            2866..5583
                     /gene="gyrA"
                     /locus_tag="VN968_09140"
     CDS             2866..5583
                     /gene="gyrA"
                     /locus_tag="VN968_09140"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HXL29437.1"
                     /translation="MSDSEDTKPGEPPAPSDIRPVSILDEMKRSYLDYAMSVIVARAL
                     PDARDGLKPVHRRILYSMYEQGHTPDKKYVKSARVVGDVIGKYHPHGDQSIYDAMVRM
                     AQDFSMRVPLIDGQGNFGSVDGDPPAAYRYTEARLARSAMAVLGDIDKDTVDFQPNYD
                     SSESEPRVLPAKFPNLLVNGAGGIAVGMATNIPPHNLGEVVDACVALIENPALTIDEL
                     INIIPGPDFPTGGIILGRQGIRSAYHLGRGSIVMRGKVSIETIRKDREAIVISEIPYQ
                     VNKATMVERIGELHREKKIEGITDLRDESDRDGFRVVVELKRDAMADVVLNQLYRFTP
                     LQTNFAANVLALDSGRPQVMNLKDLLTLFIAFREQVITRRTKFLLNKARDRAHILVGL
                     AIAVANIDEIIRVIRTSPDPNTARETLMSRDWPAKDVAAMITLIDDPRHRLAEDGTAR
                     LSMEQAKAILELRLARLTALGREEISEELDKLAVEIADYLDILRSRARVQTIVKDELA
                     AVKSEFATPRKTVIIEHEDEVEDEDLIQREDMVVTVSHAGYVKRVPLSTYRAQRRGGK
                     GRSGMQTRDEDFVSRLFVASTHTPVLFFSSRGQVYKEKVWRLPVAAPNGRGKALINIL
                     PLEQGERITTIMPLPEDESSWANLDVMFATTGGNVRRNKLSDFVDVRRSGIIAMKLDA
                     DEAIVDVQICTEHDDVLLTAAGGQCIRFPVTDVRVFQGRTSMGVRGIALPANDKLISL
                     TILRHMDADAEERAAYLRRANAVRRGGVEEDAGTDSEEASGAIELGEQRYVEMSAHEQ
                     FVLTISENGYGKRSSSFEYRTTGRGGKGIVAMSVNGRNGKLVASFPVEDSDQIMLVTD
                     KGQLIRCPVEGIRIAGRSTQGVIVFDTAEDEHVVSVEHIGEDTENGNGG"
     gene            complement(5655..>6772)
                     /locus_tag="VN968_09145"
     CDS             complement(5655..>6772)
                     /locus_tag="VN968_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HXL29438.1"
                     /translation="ALVLNYFGQAALLITDASALDHPFYQLAPDWLHYPLVAFATVAT
                     VIASQAIISGVFSLTQQSIQLGFLPRMQIKHTTSHAMGQIYVPLVNWLLAAATLGAVI
                     GFGTSDALAGAYGIAVSLLMAITTLLAALVAIQWGYSPVIVIAVNGFFFVIDIIFFAA
                     NSTKLFEGGWFPLLLAAVIAFLMLTWRSGVKLVERARGRLRQPEEDLIETAVNRCRAR
                     LSGTAVFLASAPKGVPLALTEFVKHNHVLHQRVVLVTVLIEEAPRIADEDRAEIIEVI
                     EGITRVILHYGFMQQPTIFEGLKLACEQGKLPGIDLTDITYYIGRETIIPSEDIPGMW
                     VWRETVFAFLQRNAERSAAFFGVPTRQVVEFGTEIEI"
BASE COUNT         1339 a         2047 c         2108 g         1278 t
ORIGIN      
        1 gccttgatca cttcatccat atcggcaatt ccaggcgcgt tgaccgcgcg cgttagggcg
       61 cgcatcgccg gaacagccgg gatcggacgc caatgggtgc gctcggaaca taggaagaac
      121 gggaaacgat ttccagtctt ccttgcaagc catcaacggt ttgttgtggg cggggttgcc
      181 gtgcagatgt catgagatgt caggattgga aaaccccgaa cggaaaaacg aaaaggccgg
      241 catgaaagcc ggcctttgct tatttcgaga tgatcagttc gggctggcgt cagtacttcc
      301 cgacgaccgg gccgccccaa cgatagttca cgcgagccgt gaccgtgtcg gcatcctgcc
      361 ggatgctgtc ggtccgcgta ttcacgccag ccaccggaaa cacgctggtg aaggtgttgc
      421 tctgggtgcc tatgaacata tggtcgtatt cgacggcggc cgaccagttc gaagcgaagc
      481 catattcaac gccggcgcca actgtcgcgc cccagcgcgt cgaggtcgaa cggtcaagga
      541 ccgttccggt tccagtgaga aggccctcgt atttgttgtc ggtgaccgca gcgccgccct
      601 ttgcgtacag aagggcatta ttccaggcaa agccgatctg gccggtgaac aggccgaagg
      661 cgtcgaggcg tgtgcggttg gtggctccgg gcgccgccaa gctcgggttt gatccgcgca
      721 gatcggccca gttgccttgc gcttccaatc cgtaaacgaa accgccggtc tgccagcgat
      781 atcccagctg accgcccgcc gtgccgccgg tcgcatcgtg gcagccttcg gcagcggcag
      841 gaatggcgac tccgccgatt gcggtattgg tatagcaatt gtggctggtt ccccagccgc
      901 cgttggcgcc gacgtagaag ccgctccagt catagaccgc agcgatcatc gcgggtgcct
      961 tggtgtacgg acgggctgca agatcggcag ccgaggcggg cgcagcgaag ctcagcgcaa
     1021 ccaagcccac cgcacccagc aaaaacttct tcatccaaat ctctccagtt cttatccgct
     1081 tcgcttggtc cccgaagcgt ttttcaattc cgaccgccga gacgatccaa ctgatggtcg
     1141 caatatatcg gtttcaacag gtaaagccgt ctccgaaata caacactcgg gaaggaagtg
     1201 gatcggacgg aagttatgga agttaatgat ttatttggga cattcgcaaa cctgatggga
     1261 acttgtcgcg ggtcgggacc ccctcctggc cgtcgtaggt tctataatac ctgatgggga
     1321 ttttctcggc gattgtttta tggaacaaaa ctggaacggc gacggaagca gtgctatatg
     1381 tggataaccg gggatcgccg ggctcgcggg agcggcaagc gtatagggtc ggttcaaggt
     1441 ggtcccggga agtcccaagg aagtctcaag acaatacctt gcgctcaagc caacggctga
     1501 gcgtccggcg agaccggtga ttttgccagt gaatttcgag agtggagagc ggcgatggcg
     1561 ggaagcgtga acaaggtcat tctgattggc aatctcgggg ccgatccgga aatcaagcgg
     1621 acgcaggatg ggcggccgat cgccaatctg cgcatcgcga catcggacac ctggcgggac
     1681 aagaacaccg gcgagcgcaa ggaaaaaacc gaatggcatc gcgtggtgat cttcagcgaa
     1741 ggcctgtgca agatcgccga gcagtatctg aagaagggcg ccaaggttta catcgaaggc
     1801 cagttgcaga cgcgcaaatg gaccgaccag agcggcgtcg agaaatactc gaccgaagtc
     1861 gtcctgcaga atttcaactc cacgctgacc atgctcgatg gtcgcagcgg cggcggtggc
     1921 ggcaatttcg gctccgatga ttccggcggc gattttggct cctccggtcc gtcaaccgcc
     1981 gcaccgcgtc gcgcggtcgc ggccggcgcc cgcaacagcg acatggacga cgatatcccg
     2041 ttctgagcct gcgatattct tgcattcatg cttgcgcatc ggccaggccg atgccgccgc
     2101 cggcggtcgt ctggatggtg acgcgcccgg cgacccgcga agcgaattga acttgacagt
     2161 ggaaagataa atgtaaatac tcgttacatc gatctcttcg gggccgtgct cccggtgttt
     2221 tggatgtggt gtttcagatg tcgcttgccc cgctgttgga cgccgctccg gcgatccccc
     2281 ttcatgcctt cgcggcgatg accgccttcg tgcttggcgt ggtccagttc gcggctccga
     2341 agggaacgct gcctcatcgc accaccggct ggatctgggt gtgcctgatg gcggccgtgg
     2401 cggtcagttc gttctggatc catcagatca ggctggtcgg cccgtggagc ccgatccatc
     2461 tgctgtcgat cttcacgctg gtggtgcttc cactcggtgt gtggatggca catcgtcacc
     2521 gggtcgccga ccatcgccgc atcatgatct tcattttttc cggggcgctc gtcattgccg
     2581 ggctgttcac gctgctgccg gggcggatca tgcacagcgt cgtgtttggt cactgatagc
     2641 gcctgcaaaa acgctgtccc gcgcgaggcg tttttcgttg ccaaccggcc ccgccggagc
     2701 gcctccaacc accgcttccg tggcccgcgc aagggggcac cgccgggctc gtaagtctct
     2761 ggaaaaacga ggggaaaatc cgcttgcgcg gcgccgttta ggggtggttt gggcaccttc
     2821 gatacgctat atgattcgga tcaagaactg accggatttc cccctttgtc tgatagtgaa
     2881 gatacgaagc ccggagagcc gccggcgccc tcggatattc gtcccgtatc catcctcgac
     2941 gaaatgaaac gctcctacct cgattacgcc atgagcgtga ttgtggcgcg cgcgctcccc
     3001 gatgcgcgcg acggtctcaa gcctgtgcat cgccgcattc tgtattcgat gtatgagcaa
     3061 ggccacacgc ccgacaagaa gtacgtcaag tccgcgcgcg tcgtcggcga cgtgatcggt
     3121 aagtaccatc cgcacggcga ccagtcgatt tacgatgcga tggtccggat ggcgcaggat
     3181 ttctccatgc gcgtgccgct gatcgacggg cagggtaatt tcggctcggt cgacggcgat
     3241 ccgccggcgg cttatcgata tacggaagcg cgtctcgccc gatccgcaat ggcggttctc
     3301 ggggacatcg acaaggacac cgtcgatttc cagcccaatt acgacagttc cgagagcgag
     3361 ccgcgcgttc ttccggccaa attcccgaac ctgctggtca acggcgctgg cggcatcgcc
     3421 gtcggcatgg cgaccaatat tccaccgcac aatctcggcg aagtggtcga cgcctgcgtg
     3481 gcgctgatcg agaatcccgc gcttaccatc gacgagctca tcaatatcat ccccggaccg
     3541 gattttccga ccggcggcat catcctcgga cgccagggca tccgctcggc ctatcacctt
     3601 ggccgcggct cgatcgtgat gcgcggcaag gtgtcgatcg agaccatccg caaggaccgc
     3661 gaagcgatcg tcatttcgga aattccgtac caggtgaaca aggccaccat ggtcgagcgc
     3721 atcggcgaac tccatcgcga gaagaagatc gagggcatta ccgatctgcg cgacgaatcc
     3781 gaccgcgatg gtttccgcgt cgttgtcgaa ctgaagcgcg atgcgatggc ggacgttgtg
     3841 ttgaatcagc tctatcgctt cacaccgttg caaacgaatt ttgccgccaa tgtgttggcg
     3901 ctcgattccg gccggccgca ggtgatgaat ctgaaggacc tgctgacgct gttcatcgcc
     3961 ttccgcgagc aggttattac ccgccgcacc aaattcctgc tcaacaaggc ccgcgaccgc
     4021 gcccatatcc tggtcggtct cgccattgcg gtggccaata tcgacgagat catccgggtg
     4081 atccggacct cgcccgatcc caacaccgcg cgcgaaaccc tgatgtcgcg cgactggccg
     4141 gccaaggacg tggcggctat gatcacgctg atcgacgatc cccgtcatcg gctcgccgaa
     4201 gacggcaccg cgcggctgtc gatggagcag gccaaggcca ttctcgaact gcgtctggcg
     4261 cgcctgacgg cactcggccg cgaggaaata tccgaggaac tcgacaagct cgcggtggaa
     4321 atcgccgatt acctcgacat cctgcgttcc cgcgcccgcg ttcagacgat cgtcaaggac
     4381 gaacttgcag cggtgaagtc ggaattcgcg accccgcgca agaccgtcat catcgagcat
     4441 gaagacgagg tcgaggacga ggacctgatc cagcgcgaag acatggtggt cacggtctcg
     4501 catgccggct acgtcaagcg cgtgccgctg tcgacctatc gcgcgcaacg gcgcggcggc
     4561 aagggccgtt cggggatgca gacccgcgac gaggatttcg tcagccggct attcgtggcc
     4621 tcgacccaca ccccggtgct gttcttctcg tcgcgcggac aggtctacaa ggaaaaggtc
     4681 tggcgcctgc cggtggcggc gccgaacgga cgcggcaagg ctctgatcaa tatcctgccg
     4741 ctggagcaag gtgagcgcat caccaccatc atgccgctgc ccgaggatga atcctcctgg
     4801 gccaatctcg acgtgatgtt cgccaccacc ggcggcaatg tccgccgcaa caagctgtcg
     4861 gacttcgtcg atgtccggcg ttccggcatc atcgccatga aactcgacgc cgacgaggcg
     4921 atcgtcgatg tgcagatctg taccgagcat gacgacgtgc tgttgacggc tgccggcggc
     4981 cagtgcatcc gcttccccgt caccgacgtg cgcgtgttcc agggccgcac ctcgatgggc
     5041 gtgcgcggca tcgctttgcc ggcaaacgac aagctgatct cgctgaccat tctgcgccac
     5101 atggacgccg atgccgagga gcgcgccgcc tatctgcgcc gcgccaatgc ggtgcggcgc
     5161 ggcggcgtcg aggaggacgc ggggaccgac agcgaggagg cctccggcgc cattgaactc
     5221 ggcgagcagc gctatgtcga aatgtccgcc cacgagcaat tcgtgctgac catcagcgag
     5281 aacggctatg gcaagcgctc gtcgtcgttc gaataccgga ccaccggtcg cggcggcaag
     5341 ggtatcgtgg ctatgtcggt gaatggccgc aacggcaagc tggtggcgtc attcccggtc
     5401 gaggacagtg accagatcat gctggtgacc gacaaggggc aattgatccg ttgtccggtc
     5461 gagggcatcc ggattgccgg ccgctccacc cagggcgtca tcgtgttcga taccgccgaa
     5521 gacgagcatg tggtgtccgt cgagcatatc ggcgaggaca ccgagaacgg caacggcggt
     5581 taaagaggcg aatggcgagt agcgaataga aagttcgcct tactattcgc tattcgcact
     5641 cgctattcgc ctgctcaaat ctcgatctcg gtgccgaact cgacgacctg gcgggttggc
     5701 acgccgaaga acgcagcgga gcgttcggca ttgcgttgca ggaacgcgaa cacggtttcc
     5761 cgccacaccc acatgcccgg gatatcttcg ctggggatga tggtctcgcg gccgatgtaa
     5821 taggtgatat cggtgagatc gatgccgggc agcttgccct gctcgcaggc gagcttcaat
     5881 ccttcgaaga tcgttggctg ttgcatgaag ccatagtgca ggatcacgcg ggtaatgccc
     5941 tcgatcacct cgataatctc cgcccgatct tcgtcggcaa tgcgcggggc ttcctcgatc
     6001 agcacggtaa cgagcaccac gcgctggtgc agaacgtggt tgtgcttgac gaattccgtc
     6061 agcgccagcg ggacgccctt gggggccgac gccagaaaca ccgcggtacc cgagagtctg
     6121 gcgcggcatc tgttgaccgc ggtctcgatc agatcttctt ccggctggcg cagcctgccg
     6181 cgcgcccgct cgaccagctt gacgccgctt cgccaggtca acatcaggaa ggcgatcacg
     6241 gccgcgagca acagcggaaa ccacccgccc tcgaacagtt tggtcgaatt cgctgcaaag
     6301 aagatgatgt cgatgacgaa gaagaagccg ttgaccgcga tcacgatgac gggcgaatag
     6361 ccccactgga tcgccaccag agcggccagc aaagtcgtga tcgccatcaa cagcgacacg
     6421 gcaattccgt aagcgcccgc aagcgcgtcc gacgtgccga agccgatcac ggcgccgagt
     6481 gtcgccgccg ccagcaacca gttgaccagc ggcacataga tctggcccat cgcgtggctg
     6541 gtggtgtgtt taatctgcat gcgcggcagg aagccgagct ggattgactg ctgcgtcaat
     6601 gaaaataccc cggagatgat ggcctgcgag gcaatgacgg tggccacggt ggcgaaggca
     6661 acgagcggat agtgcagcca gtcaggtgcg agctgataga agggatggtc gagcgcgctg
     6721 gcgtcggtga tcagcagtgc cgcctgcccg aagtaattga ggacgagggc tg
//
LOCUS       DATNOI010000243        13783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1105014,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000243 DATNOI010000000
VERSION     DATNOI010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13783
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105014"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VN968_09150"
     CDS             complement(<1..528)
                     /locus_tag="VN968_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXL29439.1"
                     /translation="MAGFITIEKGLGPEGRVAVVRFDRGDGINALSPEALRQLTDAAR
                     SFEDDSGTSVVVLTGSAKAFSAGFDLKDAEGRSRSTMDLGSLRRHLKLGPRLTRAWQE
                     MEQITIGAIEGFCIGGGVALVVALDFRVMARDAHMRVPEIGLGMNMSWRSVPRMLHLI
                     GPARTKQAVILADQRI"
     gene            complement(850..1578)
                     /locus_tag="VN968_09155"
     CDS             complement(850..1578)
                     /locus_tag="VN968_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29440.1"
                     /translation="MTAAVSAWLTAGDARAANGAFAVDAADISEVGSCKVESWMSTAT
                     NSDFSAVANPSCVANIFKPVELSLLTNRSRSDGDWSTSIAPKSKWNIVPTRIGKFGFS
                     FYAGGSFDALTGENLTAFAVVPATYRLSETMRINVNAGWLWDRMADRHYLTYGLGFDW
                     KFTDVLQLTLEAFGQAGASDIPSVVQPRFQAGVRYRPNEIFSVDLIYGRNITGENANW
                     ITVGTTIRFPAPEGKPARERTGHL"
     gene            1674..2303
                     /gene="nthA"
                     /locus_tag="VN968_09160"
     CDS             1674..2303
                     /gene="nthA"
                     /locus_tag="VN968_09160"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HXL29441.1"
                     /translation="MTGHDHDHHHHDHDHSELSETELRVRALETILTEKGYVDPAALD
                     AIIQAYETKIGPRNGAQVVAKAWSDPVFKRTLLEDATQAVSALGHVSRVGDHLVAVEN
                     TPVQHNLVVCTLCSCYPWEMLGLPPVWYKAAPYRSRAVKDPRGVLADFGVSLPKETEI
                     RVWDSTAETRFLVLPMRPEGTDGWSEQQLAELVTRDSMIGTGSPKKPGG"
     gene            2306..2965
                     /gene="nthB"
                     /locus_tag="VN968_09165"
     CDS             2306..2965
                     /gene="nthB"
                     /locus_tag="VN968_09165"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HXL29442.1"
                     /translation="MNGVHDMGGMDGFGKVEPELNEPMFHAEWEARVLAMVRAMGAAG
                     AFNIDTSRFYRETLPPHVYLGSSYYRKWQLGLEGLLMDKGYITTEELVAGHAVEPAKP
                     LKHGKFTLADVERIMVRGKFGRVAPAPAKFRPGDHVRTKNIHPATHTRLPRYARGRVG
                     VIERDHGCQVFPDSAATESGENPQWLYTVVFDGTELWGPDADPSVKISIDAFEPYLEP
                     A"
     gene            2965..3351
                     /locus_tag="VN968_09170"
     CDS             2965..3351
                     /locus_tag="VN968_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HXL29443.1"
                     /translation="MSIDPQAAWHATSSLPDVPRDQDGPVFREPWEAQAFAMALALHA
                     RGLFTWTEWAQMLAKEIKRAQGSGDADTGETYYRHWLATLEKLVAAKGVATSETLHRY
                     RDAWDRAADRTPHGSPIEVRPEDFGS"
     gene            complement(3443..4015)
                     /locus_tag="VN968_09175"
     CDS             complement(3443..4015)
                     /locus_tag="VN968_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29444.1"
                     /translation="MSLTPDALTANAEVEDRLGETFGLEFDIDPEEPPWFSVDGVEAI
                     RQFAGDGSGGVFAQLGDARVLYVSSEGEAGILAADLDEFIKLLVACPYWQDVLKFSAG
                     GNLDEMRRAAAFFETARAGDEELQEARALLKSQLGLTGPSDPVGALHRAASSEVIVRG
                     PHGPPFVSLFYYSHVLDEEGMRRRFPWLYG"
     gene            complement(4121..4837)
                     /gene="queC"
                     /locus_tag="VN968_09180"
     CDS             complement(4121..4837)
                     /gene="queC"
                     /locus_tag="VN968_09180"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HXL29445.1"
                     /translation="MSDQLPSKTALVLFSGGQDSATCLAWALQRFARVEMLGFNYGQR
                     HAIELECRGRLLDGMKLLRADWAARLGESHTLEIPTLATISDTALTRDVAIAMGEDGL
                     PNTFVPGRNLVFLTFAAALAYRRGITHIIGGMCETDYSGYPDCRDETIRALQTALNLG
                     MAKTFELHTPLMWLDKAGTWKLARDLGGAGLVDVIREHSHTCYLGERGAQHAWGYGCG
                     ECPACALRARGWREYAAGAA"
     gene            4969..5784
                     /gene="mazG"
                     /locus_tag="VN968_09185"
     CDS             4969..5784
                     /gene="mazG"
                     /locus_tag="VN968_09185"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HXL29446.1"
                     /translation="MPPSRDILGLIEIMAMLRTPGSGCPWDLEQNFATIAPYTIEEAY
                     EVAEAIARGDLDDLRDELGDLLLQVVFHARMAEEQNAFSFGDVVEAISAKMIRRHPHV
                     FADRDGRLTPSDVKGAWDRIKAEEKAERAARRLGEETARKSLLSGIKAGQPALTRAME
                     LQRKASSVGFDWNDPRAVLHKIREEADEIEAALDRGDADELAQETGDLLFALVNLARH
                     VGADPDLALRGTNAKFERRFGYIERALAAQGRSLESASLAEMDALWNAAKGEE"
     gene            complement(5836..7215)
                     /gene="hflX"
                     /locus_tag="VN968_09190"
     CDS             complement(5836..7215)
                     /gene="hflX"
                     /locus_tag="VN968_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HXL29447.1"
                     /translation="MEPRNPEEGVDRPRSAAGKETGRVVVIGPYLRMRRGDPEAQADH
                     YVMRGSEARLEEAVGLARAIDLTIAEALIAPVSQIRPATYLGKGKVEEIVGLITGQEI
                     ELVVMDCALSPIQQRNLEKAWNTKVLDRTGLILEIFGRRAKTREGALQVELAHLNYQR
                     SRLVRSWTHLERQRGGFGFMGGPGETQIEADRRLIGDRIARLENEIKKVQATRRLHRA
                     GRRRVPYRVVALVGYTNAGKSTLFNRLTRADVQAADMLFATLDPTLRALSLPHGGKAM
                     LSDTVGFISNLPTQLVAAFRATLEEVLQADVILHVRDISHEDADAQQRDVDTVLHQLG
                     IDPDAGQRILEVWNKIDRFDPEMRENLQNIAARRPPERPCFLVSAETGAGIDALLTAI
                     EDRLAATRMTLDLTIDAADGAGISWLHRNSEVLNKELHDGRFDMTVRVDETKRDIVVN
                     RFDAVPHAM"
     gene            complement(7227..7475)
                     /gene="hfq"
                     /locus_tag="VN968_09195"
     CDS             complement(7227..7475)
                     /gene="hfq"
                     /locus_tag="VN968_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HXL29448.1"
                     /translation="MAADRAQNLQDTFLNHVRKTKTPLTIFLVNGVKLQGIVTWFDNF
                     CLLLRRDGHSQLVYKHAISTIMPGAPIQLFEGGEDAPA"
     gene            complement(7786..9156)
                     /locus_tag="VN968_09200"
     CDS             complement(7786..9156)
                     /locus_tag="VN968_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXL29449.1"
                     /translation="MANDILIVDDEADIRDLVAGILEDEGFTTRTARDSDSALAEIAN
                     RRPHLVFLDIWLQGSKLDGLQLLEQIKKDHAEVPIVMISGHGNIETAVAAIKRGAYDF
                     IEKPFKSDRLILVATRALETSRLKREVKELKQLAPAASVLTGRSACMNQLRQTIERAA
                     KANSRILIVGPSGAGKELAARTLHNASNRSEGPFVVINAAAITPERMEVELFGIEQSN
                     GEQPRKAGALEEAHSGTLFIDEIGDMPRETQNKILRVLVDQTFQRSGGTSKVNVDVRI
                     ISSTARNLEEEIAEGRFREDLYHRLSVVPIRVPPLSERREDIPELIDYFMDQISVATG
                     LPKRQIGQDAMAVLQSHVWPGNVRQLRNNVERVMILAGGGPEVIITADMLPQDVGSMV
                     PAMPTSNNGEHIMGLPLREAREVFERDYLIAQISRFSGNISRTAEFVGMERSALHRKL
                     KALGVG"
     gene            complement(9174..11498)
                     /locus_tag="VN968_09205"
     CDS             complement(9174..11498)
                     /locus_tag="VN968_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HXL29450.1"
                     /translation="MTSADSSAPSFDPSFAESKGGSLRRWLAPFAVGLALLSAFLTFV
                     VLTGLTPIEPTREVVVSFMLINGATILVLVGIIVREVWQVVQARRRGRAAARLHVQIV
                     SLFSVIAVLPAVLVSIVANVTIDRGLDRLFSGPTREAIQNSLTIARAYTDEHAQLIRG
                     DILGMANDIARARPLYDRHLGTFLEMLTASAASRNLPGAMLIDKDSKPLEMARTGIQQ
                     DFVTPDPELLSKVNETEPQIAVFPENYVAAVIRLRGFDDTFLYVARLLDPRVVAQLKQ
                     TQASVAEYAEIESRRLGLQVAFGLMFAVIALTILMASVLIGLNFANWLVAPIRRLMSA
                     ANIVSTGDLHVQVPVHKSEGDLAQLGETFNKMTQELRSQRDELVSASDLIDSRRRFIE
                     AVLSSASAGIIGVDASGNVGILNRSAEKLIGHAESETLDHPLSDVLPELDDMMKAARE
                     GTQRLVQGQITIMRDGHERNLSVRVSAEQTSQSRDSYIITLDDITELVSAQRTSAWAD
                     VARRIAHEIKNPLTPIQLSAERIRRKFGKVITEDKAVFEQCTDTIVRQVDDIRRMVDE
                     FSRFARMPKPVIEGEDVGDTVRQAVFLMRVGHPDIDIEAAIKDEPMRAQFDRRLISQA
                     LTNIIKNATEAIEAVPADELGKGRIEVVAARENDDIVIDVIDNGIGLPKVSRARLLEP
                     YVTTREKGTGLGLAIVGRVLEDHGGRIELNDAADIRPGARGAWMRLRFAVSGHAAKVE
                     VKEPAAETKKAAYETAGPAEATNNETRIEAATGG"
     gene            complement(11674..13116)
                     /gene="ntrC"
                     /locus_tag="VN968_09210"
     CDS             complement(11674..13116)
                     /gene="ntrC"
                     /locus_tag="VN968_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HXL29451.1"
                     /translation="MPAGSILVADDDTAIRTVLNQALSRAGYEVRLTGNAATLWRWVS
                     QGEGDLVITDVVMPDENAFDLLPRIKKMRPNLPVIVMSAQNTFMTAIRASERGAYEYL
                     PKPFDLKELIAIVGRALAEPKERVANHADESEFDSIPLVGRSPAMQEIYRVLARLMQT
                     DLTVMISGESGTGKELVARALHDYGKRRNGPFVAVNMAAIPRDLIESELFGHERGAFT
                     GANTRASGRFEQAEGGTLFLDEIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRI
                     VAASNKDLRILIQQGLFREDLFFRLNVVPLRLPPLRERIEDLPDLIRHFFALAEKDGL
                     PPKKLDALALERMKQHRWPGNVRELENLARRLAALYPQDVITGSVIDGELAPPAVTSG
                     GSVHHGVDNLGGAVEAYLSSHFSGFPNGVPPPGLYHRILKEIEVPLLTAALAATRGNQ
                     IRAADLLGLNRNTLRKKIRDLDIQVYRSGN"
     gene            complement(13126..>13783)
                     /locus_tag="VN968_09215"
     CDS             complement(13126..>13783)
                     /locus_tag="VN968_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL29452.1"
                     /translation="RMLTRLICDEADRIVTLVDRMEVFGDDRPVARGPVNIHSVLDHV
                     KRLAQSGFARNIRFVEEYDPSLPPVLANQDQLIQVFLNLVKNAAEAVVDLGSDAEIQL
                     TTAFRPGVRLSVPGKKSRVSLPLEFCVKDNGPGVPEDLLPNLFDPFVTTKPTGSGLGL
                     ALVAKIVGDHGGIIECESQPRKTTFRVLLPMFNSAKHIDHGNRDHASGTPSHASQDTR
                     "
BASE COUNT         2597 a         4387 c         4188 g         2611 t
ORIGIN      
        1 aatgcgctga tcggccagga tcaccgcctg cttggtccgg gcgggcccga tcaaatgcag
       61 catgcgcggc acgctccgcc agctcatatt catgccgagc ccgatttcag gcacccgcat
      121 gtgggcgtcg cgggccatga cgcggaagtc cagcgccacc accagcgcca cgccgccgcc
      181 gatgcaaaaa ccttcgatcg cgccgatggt gatctgctcc atctcctgcc aggcgcgggt
      241 caggcgcggt ccaagcttta agtgccggcg caacgacccg agatccatgg tgctgcgtga
      301 ccggccctcg gcgtctttca ggtcgaagcc ggcgctgaat gccttggcgc tgccggtgag
      361 cacgacgacg gaagtgccgc tgtcgtcctc aaaactgcgc gccgcatcgg tgagctgacg
      421 cagcgcttcc ggcgacagcg cgttgatacc gtcgccacgg tcgaagcgca ccacggcgac
      481 gcggccctcg ggcccgagac ccttttcgat ggttatgaac cccgccaacg tccgcgctcc
      541 ctgcccgcta ttttcttgca acgatacctc acaaatgcgg tttatcactt gtcgggaaga
      601 aaaatttccg cgacctcata catcccctga tcgaacgtcg ctatcttgag cgctttctcc
      661 agcgcaggcg ctcgcgatcg ggacttatct cgtcgtcaac cggcttggcg aattttatgt
      721 actctcgatc gcgttcggga cggcatatgg cgcggtctcg tgaacggctt caggcggctt
      781 aagcaagcac ctgggcaagc aagcacctgg gcaggcaagc atctgggcag gcaagcatct
      841 gggaacggct cacaaatgcc cggtacgctc gcgtgccggc ttgccttccg gcgccggaaa
      901 ccggatcgtg gtcccgaccg tgatccaatt ggcgttctcg ccggtgatgt tgcggccgta
      961 gattagatcg accgagaaaa tctcgttggg ccggtaacgc acgccggcct gaaaccgcgg
     1021 ctgaaccacg cttgggatat ccgatgcgcc ggcctggccg aaggcttcca gcgtcagctg
     1081 caatacatcg gtgaatttcc agtcgaagcc aaggccatag gtcaggtaat gccggtcggc
     1141 catgcggtcc cacagccagc cggcattgac gttgatccgc atggtttcgg acagcctgta
     1201 ggtcgcaggc accaccgcga aggccgtgag attttcgccg gtcagcgcgt cgaacgagcc
     1261 gcccgcatag aatgaaaatc cgaattttcc gattctggtg ggaacgatat tccacttcga
     1321 cttgggagcg atactggtgc tccagtcgcc gtcgctgcgg gaccggttgg tcagcaggct
     1381 cagttccacc ggcttgaaga tattggctac gcaggaggga ttggccaccg ccgaaaaatc
     1441 gctgttggtc gcggtcgaca tccagctctc gaccttgcac gatccgactt cggaaatgtc
     1501 ggcggcatcc accgcgaagg caccgttggc cgcccgcgcg tcgcccgccg tgagccacgc
     1561 cgaaacggcc gccgtcacgc gaaatgcgat gcgtagcccc caaaacccca ttcgcaaggg
     1621 ctatcagagt ctgtgcctcg acatagcccg aatattgcgt ttatcctcag cccatgacag
     1681 gccacgatca cgatcatcat caccacgacc atgaccattc ggaattgtcc gagaccgagc
     1741 tgcgggtgcg cgcgctggaa accatcctga ccgaaaaagg ctatgtcgat ccggcagcac
     1801 tcgatgccat tatccaggcc tacgaaacca agatcggtcc ccgcaacggc gcccaggtcg
     1861 ttgccaaagc ctggtccgat cctgttttca agcgcaccct gctggaagac gccacccagg
     1921 ccgtcagtgc gcttggtcac gtcagccgag tcggcgatca cctcgtcgct gtcgagaaca
     1981 cgccggtgca gcacaacctg gtcgtgtgca cgctgtgttc atgctatcct tgggaaatgc
     2041 tggggcttcc tccggtctgg tacaaggcgg cgccctaccg ttcccgtgct gtaaaagatc
     2101 cgcgcggcgt gctcgccgac ttcggcgtga gcctgccgaa ggaaaccgag attcgggtgt
     2161 gggattcgac cgccgagacg cgcttcctgg tgctgccgat gcgacccgaa ggcaccgacg
     2221 gctggagcga acagcagctc gccgagctcg tgacgcgcga ctccatgatc ggcaccgggt
     2281 cgcccaaaaa gccgggtggc tgatcatgaa tggcgtgcac gacatgggcg gcatggacgg
     2341 ctttggcaag gtcgaaccgg aactgaacga gccgatgttt catgccgaat gggaagcgcg
     2401 cgtgctggcc atggtgcgcg caatgggtgc ggcgggcgca ttcaacatcg acacctcacg
     2461 cttctatcgc gagacgttgc cgccccacgt ttatctcggc agttcatact acaggaaatg
     2521 gcagctcggg ctcgaaggcc tgctgatgga caagggctac atcacaaccg aggaactcgt
     2581 cgccggtcac gcggtagagc cggcgaagcc tcttaaacac ggcaagttca cgctcgccga
     2641 tgtcgagcgc atcatggtgc gcggcaaatt cggacgcgtt gcccctgccc cggcgaaatt
     2701 caggcccggc gatcacgtgc gcacgaagaa tatccatccc gcaacacaca cgcggttgcc
     2761 tcgttacgcg cgaggccgcg tcggcgtgat cgaacgcgac cacggctgtc aggtttttcc
     2821 ggactcggcc gcgacggaat ccggtgagaa cccgcaatgg ctttacaccg tcgtattcga
     2881 tgggaccgaa ttatggggtc cggacgccga cccgagtgtc aagatatcga tcgatgcgtt
     2941 cgagccctat ctggagccgg cctgatgtcg atcgatccgc aggccgcatg gcacgccacg
     3001 agctcgttgc cggatgttcc ccgcgaccag gatggcccgg tgtttcgcga accctgggaa
     3061 gcacaggcct tcgcgatggc actcgccctg cacgcgcgag ggctgttcac ctggaccgaa
     3121 tgggcgcaaa tgcttgccaa ggaaatcaag cgtgcccagg gatcaggcga tgccgacacc
     3181 ggcgagacct actaccggca ttggctcgcg acactggaga aactcgtcgc cgcgaaaggc
     3241 gttgcaacgt ccgaaaccct gcatcgctat cgcgatgcct gggaccgcgc cgccgatcgc
     3301 acgccacatg gttcgccgat cgaagtgcgg ccggaggatt ttggaagcta gcctggcgtg
     3361 atctctcccc ccgtccccgc aaaaaagggg cgagggaaaa gaaacccgac cgcggctgac
     3421 aacccctcgg gaatcaatcc ctttacccgt agagccaagg gaaccgcctc cgcatgccct
     3481 cttcgtcgag gacatgggag tagtagaaca aggaaacaaa cggcgggcca tgtggccccc
     3541 gaacgatcac ctccgaggac gctgcgcgat gcagcgcacc aactggatcg cttggccccg
     3601 tgagcccgag ttgcgatttc aacagagcac gcgcctcttg caactcttca tcgcctgccc
     3661 tcgcggtttc aaagaaagct gcggcccggc gcatctcgtc gaggttgccg cccgcggaaa
     3721 atttgaggac atcctgccaa tagggacagg cgacgaggag tttgatgaac tcgtcgagat
     3781 cggcggcgag aataccggcc tccccttcag acgagacgta aagcacgcgc gcgtccccga
     3841 gctgcgcgaa cacgcccccc gagccgtcac cagcaaattg cctgatcgct tcaactccat
     3901 ccaccgaaaa ccacggcggc tcctccggat cgatgtcaaa ttccagaccg aaggtctcgc
     3961 ctaatcgatc ctcgacttcc gcattcgcgg tcaacgcatc aggggtaagg gacattcgga
     4021 tccatcacgg ggtcgttgat gcaggccaaa tttgtcgcaa gatgacgacg gcaggttcgg
     4081 tcgtgccttg agaaagttgc ctcgccggct cacccaacgg ctaagccgcg cccgccgcat
     4141 attcccgcca tcccctggcg cgcaacgcgc aggccgggca ttcgccgcaa ccatagcccc
     4201 acgcgtgctg cgcgccgcgt tcgccgagat agcaggtgtg ggaatgttcg cggatcacat
     4261 cgaccagccc tgccccgccg aggtcacgcg ccagtttcca ggtgccggcc ttgtccagcc
     4321 acattagcgg cgtatgcaat tcgaacgtct tggccatgcc gagattgagc gcggtctgca
     4381 acgccctgat ggtctcgtcg cggcaatccg gatagccgga ataatcggtc tcgcacatcc
     4441 cgccgataat gtgcgtgatg ccgcgccgat aggccagcgc agccgcaaat gtcaggaaca
     4501 ccaggttgcg gccgggcacg aaggtgttgg gcaggccgtc ctcgcccatc gcaatcgcga
     4561 cgtcgcgcgt cagcgcggtg tcggaaatcg tggccaatgt cggaatctcc agggtgtggc
     4621 tctcgccgag tctagccgcc cagtccgcgc gcaataattt catcccatcg agcaggcggc
     4681 cgcggcattc aagttcgatg gcgtgtcgct ggccgtaatt gaaaccgagc atttccacgc
     4741 gcgcaaagcg ctgcagcgcc caggcgagac aggtggcgga atcctgtccg cctgaaaata
     4801 gcaccagcgc ggtctttgat ggcaattgat cgctcatcgg ctgcccttag caccgtcagg
     4861 cgctcgcgcc aatcggtgga aaccacaaac ccattcactt gttttcgctg ggcgggcagc
     4921 acgtcttgcg gcatacaaag cctggttttt cgcaagcttc ggtgtaccat gcccccttcc
     4981 cgcgatattt taggcctgat cgagatcatg gcgatgctgc gaacccccgg cagcggttgc
     5041 ccttgggacc tcgaacagaa tttcgcgacc atcgctccct acaccatcga ggaggcctat
     5101 gaagtggccg aggcgatcgc gcgcggcgat ctcgacgatc tccgcgacga actcggcgat
     5161 ctcctgctgc aggtggtgtt tcatgcccgc atggccgaag agcagaatgc gttttcattc
     5221 ggcgacgtgg tcgaggcgat cagcgccaag atgatccgaa ggcatccgca tgtcttcgcc
     5281 gacagggatg ggcgattgac gccttcggat gtgaagggcg cgtgggaccg catcaaggcc
     5341 gaggagaagg ccgagcgcgc ggcgcggcgg cttggggaag aaaccgcgcg caaatcgctg
     5401 ctgtcgggca tcaaggcggg acagccggcc ctgacgcgcg cgatggaatt gcagcgcaag
     5461 gcgtcaagcg tcggcttcga ctggaacgat ccgcgtgcgg tattgcacaa gattcgcgag
     5521 gaagccgacg agatcgaggc cgcgctcgac cgcggcgatg ccgacgaact cgcccaggaa
     5581 accggcgatc tgctgttcgc gctggtcaat ctggcgcggc atgtcggcgc cgatcccgat
     5641 ttggcgctgc gcggcaccaa cgccaaattc gaacggcgct tcggctatat cgaacgcgcg
     5701 ctcgcggcgc agggccgctc gctcgaaagc gcctcgctcg ccgagatgga cgcgttgtgg
     5761 aacgcggcga agggtgaaga gtaaagagag cagccccgtc ctcgcaatga cgacggttct
     5821 gacggcgaaa gtcgttcaca tcgcgtgcgg caccgcatca aaccggttga ccacgatatc
     5881 gcgcttagtt tcatcgacgc gcacggtcat gtcgaaccgt ccgtcgtgaa gctccttgtt
     5941 gagcacttcg gaatttcgat gcagccagct gatgccggcg ccgtcggcgg cgtcgatcgt
     6001 aagatccagc gtcatgcgcg tggcggccag ccggtcttcg atcgcggtca acagcgcgtc
     6061 gatgcccgcg ccggtttcgg ccgagaccag gaaacacgga cgctccggcg ggcggcgggc
     6121 agcgatattt tgcaggtttt cccgcatctc cggatcgaag cgatcgatct tgttccagac
     6181 ctcgagaatg cgctggcccg catccggatc aatgccgagt tgatgcagca ccgtatcgac
     6241 gtcgcgctgc tgcgcgtccg cgtcctcatg cgagatgtcg cggacgtgga gaatgacgtc
     6301 ggcttgcagc acttcctcca gcgtcgcacg aaacgccgca accagttgcg tcggcaggtt
     6361 ggaaatgaag cctacggtat ccgacagcat cgccttgccg ccatgcggca gactgagcgc
     6421 acgcaaggtt gggtccagcg tggcgaacag catgtcggcg gcctgcacat cggcgcgggt
     6481 caggcgattg aacagcgtcg acttgccggc gttggtgtag cccaccagcg ccacgacccg
     6541 atacggcacc cgccgccggc cggcgcgatg cagccgccgc gtcgcctgaa ctttcttgat
     6601 ttcgttttcg aggcgcgcga tgcggtcgcc aatcaggcgg cggtcggctt caatctgcgt
     6661 ttcaccggga ccgcccatga atccgaaacc gccgcgctgg cgctcgagat gggtccatga
     6721 ccgcaccagg cgacttcgct ggtagttcaa atgcgcgagt tcgacctgca gtgcgccttc
     6781 cctggtcttg gcgcggcggc cgaagatttc cagaatcagc ccggtgcggt cgagtacctt
     6841 ggtgttccag gccttctcaa gattgcgctg ctggatcggc gacaacgcgc aatccatcac
     6901 caccagttca atctcctggc cggtgatcag tccgacgatc tcctcgacct tgcccttgcc
     6961 gagatacgtc gcgggccgga tctgactgac cggcgcaatc agcgcctccg cgatcgtcag
     7021 atcgatagcg cgggcgagac ccacggcctc ctcgagccgc gcctccgaac cacgcatgac
     7081 ataatgatcc gcttgcgcct ccggatcgcc gcggcgcatg cgcaagtatg ggccgatgac
     7141 gactacccgc ccggtctctt tgcccgctgc cgaccgtgga cggtcgacgc cctcttcggg
     7201 attacggggt tccaatcaga tcactctcaa gccggagcat cctcgccgcc ttcgaacaac
     7261 tggatcggtg cgcccggcat gatggtcgag atcgcgtgct tgtagacaag ctgcgaatga
     7321 ccatcgcgcc gcagcagcag gcagaaattg tcgaaccagg taacaatccc ctgcagcttt
     7381 actccgttga ccagaaagat cgtcagtggc gtcttggttt tgcgaacgtg attgaggaag
     7441 gtgtcttgta ggttttgtgc gcggtctgcc gccattgttt ttatcccgca gtttgtgctt
     7501 cttttgtgct tctttttatt gaaccggacg ctggccctct cgcggagcgt gacccggttg
     7561 ccgacttccc ctgagatccc cctccgggag gtacggcagg acgattagaa ggcaggcgtg
     7621 gtttttaggc aagccagttc gcgcattggc cacaggcgca attctccgta agaatacgga
     7681 aatagatgaa atttctcaag aaaatgcatg gaatgaatgc gtcgatgcgg tgtttaacga
     7741 ccggcgagcg gccatatttc ggattcgaaa ttctcaggtc ggtattcagc cgacgccgag
     7801 ggccttgagc ttgcgatgca gggctgaacg ctccatgccg acaaactccg ccgtgcgcga
     7861 gatattaccg gagaatcggc tgatctgggc gatcaaatag tctcgttcga acacttcccg
     7921 cgcctcacgc agcggcaagc ccatgatgtg ctcgccatta ttgctggtcg gcattgccgg
     7981 gaccattgag cccacgtcct gcggcagcat atcggccgta atgatgactt ccggaccacc
     8041 gcccgccagg atcatcacgc gctcgacgtt gttgcgaagc tgccggacat ttccgggcca
     8101 gacatgcgac tgcaataccg ccatcgcgtc ctggccgatc tggcgcttcg gcagcccggt
     8161 agcgaccgag atctgatcca tgaaatagtc gatcagttcg gggatatcct cgcggcgctc
     8221 cgatagcggc ggcacgcgga tcggcaccac cgacaaccga tgatagagat cttcgcgaaa
     8281 ccgtccctcc gcgatctctt cttccagatt gcgcgccgtc gaggaaatga tgcgaacgtc
     8341 gacattgacc ttgctggtac cgcccgaccg ctggaacgtc tgatcgacca gcacccgcag
     8401 gatcttgttc tgggtttcgc gcggcatgtc gccgatttca tcgataaaca acgtgccgct
     8461 atgcgcctcc tccagcgcac cggccttgcg tggctgttcg ccattggact gttcgatacc
     8521 gaacagttcg acttccattc gctccggcgt aatcgccgcg gcgttgatga cgacgaaagg
     8581 tccttcggat cgattcgatg cgttatgcag cgtgcgcgcc gccaattcct ttccggcacc
     8641 ggacggcccg acgatcagga tgcggctatt ggccttggcg gcccgctcaa tggtctggcg
     8701 cagttggttc atgcaggccg agcggccggt caaaacgctg gccgccggcg cgagttgctt
     8761 gagttccttc acttcgcgct tcaggcgcga ggtctccagg gcccgcgtcg ccaccagaat
     8821 caaccggtcg gacttgaaag gcttttcgat aaagtcgtag gcgccgcgct tgatcgccgc
     8881 gaccgcggtc tcgatattgc cgtgcccgga gatcatcacg atcggcacct cggcatggtc
     8941 cttcttgatc tgttccagca gctgcaaccc gtcgagcttg ctgccctgca gccagatgtc
     9001 gagaaacacc agatgcgggc ggcgattggc aatttccgcg agtgccgaat cgctgtcgcg
     9061 cgcggtccgg gtcgtaaaac cctcgtcttc gagaatcccc gcaaccagat cgcgaatatc
     9121 ggcttcgtca tcgacaatca gaatgtcatt tgccatcggt cacgcctgtc cggtcagccg
     9181 cctgttgcgg cttcgattct tgtttcattg ttggtcgcct ccgccggccc cgcggtttca
     9241 tacgccgctt tttttgtctc tgcggccggc tctttcactt cgactttcgc ggcgtggccc
     9301 gacaccgcaa accgcaaccg catccaggcc ccgcgcgcgc ccggtcgaat gtccgcggca
     9361 tcgttgagct cgatccggcc gccatggtct tccagaacgc ggcccacaat cgccaggccg
     9421 aggccggtgc ccttttcgcg cgtcgtgacg tagggttcga gcaggcgcgc ccggcttacc
     9481 tttggcaggc cgatgccgtt gtcgatgaca tcgatcacga tgtcatcgtt ctcgcgcgcc
     9541 gcaacaacct cgatacgtcc cttccccagc tcgtccgccg ggacggcctc gatcgcttcg
     9601 gttgcgttct tgatgatgtt ggtcaaggct tgcgatatca gccgccgatc gaactgcgcc
     9661 cgcatcggct cgtccttgat cgcggcttcg atatcgatgt cgggatgccc gacccgcatc
     9721 aggaataccg cctgccgcac ggtgtcgccg acatcctcgc cttcgatcac aggcttcggc
     9781 atccgcgcga agcgcgagaa ttcgtcgacc atccgcctga tatcatcaac ctgccgcaca
     9841 atggtgtccg tgcattgctc gaagacggcc ttgtcctccg tgataacctt gccgaatttg
     9901 cgacggatgc gttcggcgga gagctgaatt ggcgtcagcg gatttttgat ttcgtgcgcg
     9961 atgcggcgcg cgacatcggc ccatgccgaa gtccgttgtg cggaaaccag ttcggtaatg
    10021 tcgtcaagcg tgatgatgta gctgtcgcgc gactggctgg tttgttcggc gctgacgcga
    10081 accgacagat tgcgttcatg gccgtcacgc ataatcgtga tctggccctg gaccagccgc
    10141 tgggtgccct cgcgcgcggc tttcatcatg tcatcgagtt cgggcagcac gtccgacaac
    10201 ggatgatcca gcgtctccga ttcggcgtgc ccgatgagtt tctcggcgga gcggttgagg
    10261 atgccgacat tgccggaggc atcgacaccg atgatgccgg cgctcgccga cgacaacacc
    10321 gcctcgataa accggcgacg gctgtcgata aggtcgctcg cgctcaccag ctcgtcgcgc
    10381 tggctgcgca attcctgcgt catcttgttg aaggtctcgc cgagctgcgc aaggtcgcct
    10441 tccgacttgt ggaccggaac ctgcacatgc aggtcgccgg tcgagacgat gttggcggca
    10501 ctcatcagcc gccggatcgg cgcgaccagc cagttggcga aattcagtcc gatcagaacc
    10561 gaggccatca ggatggtcaa ggcgatcacc gcgaacatca gcccgaacgc cacctgaagg
    10621 ccgagccggc gcgattcgat ctcggcgtat tcggcaacgc tggcctgggt ttgcttgagc
    10681 tgcgcgacca cgcgcggatc gagcagccgg gccacgtaga ggaacgtgtc gtcgaacccg
    10741 cgcagccgga tcacggccgc gacgtagttt tcggggaaca ccgcgatctg gggctcggtt
    10801 tcgttgactt tgctcaggag ttccgggtcc ggcgtcacga agtcctgctg aatgccggtt
    10861 cgcgccatct ccagaggctt gctatccttg tcgatcagca tcgctcccgg caaattacgc
    10921 gatgcggcac tggccgtcag catctcgaga aacgttccga gatgccgatc gtacaatggc
    10981 cgggcgcggg cgatatcgtt ggccattccc agaatgtcgc cgcgaataag ctgcgcgtgt
    11041 tcatccgtat aggcgcgggc aatggtcagg gagttctgga tcgcttcacg cgtcggacct
    11101 gaaaacagcc gatcgaggcc gcgatcgatg gtcacattgg cgacgatcga caccagcacg
    11161 gccggcaaca ccgcaatgac ggaaaacagg ctgacaatct ggacgtgcag ccgcgccgcc
    11221 gcccggccgc gccgacgcgc ctgcaccacc tgccagactt cacggacgat gatgccgacc
    11281 agcaccaaga tggtcgcgcc attgatcagc atgaaggaaa cgacaacttc gcgtgtcggt
    11341 tcgatcgggg tcaggccggt gagcaccacg aaggtgagaa acgcagacaa cagggccagg
    11401 ccgacggcaa acggcgccag ccatctccgc agggaccccc ccttggattc ggcgaacgac
    11461 ggatcgaacg atggagccga gctgtctgcg ctggtcattt aggcaatggt gagctgaagg
    11521 gccttagatc cgccgctagc gaactgatgc attcatatca caatgttgcc aaattacgac
    11581 gattccgcgg cgtgtcccgc ggttggactg cattggcctg cagctttcgc catcaccata
    11641 ccgctgcgat cgggactcag ggcgcccgcg gccctaatta ccgctccggt aaacctggat
    11701 gtccaggtcg cgaatcttct tccgcagcgt gttgcggttg aggccgagca ggtcggcggc
    11761 gcggatctgg ttgcccctgg tcgccgccag cgccgccgtc agcagcggca cctcgatttc
    11821 cttcaggatc cggtgataga ggcccggcgg cggcacgcca ttggggaacc cggaaaaatg
    11881 cgacgataga taggcttcga cggcgccgcc gagattgtcg acaccgtggt ggacgctgcc
    11941 gcccgaggtc acggcgggag gcgccaactc gccgtcaatg accgagccgg tgatgacgtc
    12001 ttgcggatac aatgccgcga gccggcgcgc gaggttttcg agctcgcgca cattgccggg
    12061 ccagcgatgc tgcttcatgc gttccagcgc cagcgcatcg agcttcttcg gcggcaatcc
    12121 gtctttctcg gccagcgcga aaaaatgccg gatcaaatcg ggcaaatcct cgatccgctc
    12181 gcgcagcggc ggcaggcgca acggcacgac attcaggcgg aagaacaaat cctcgcggaa
    12241 caaaccttgc tgaatcagga tccgcagatc cttgttgctc gcggcaacga tgcgcacatc
    12301 ggtcttgatc ggtgtgcgac cgcccacggt ggtgtactcg ccctgttgca ggacccgtag
    12361 cagacgggtc tgcgcctcca tcggcatgtc gccgatttcg tcgagaaaca gcgttccgcc
    12421 ttcggcctgc tcgaaccggc ccgaggcgcg ggtgttggcg ccggtgaagg cgccgcgttc
    12481 atggccaaac agttcggact caatgaggtc gcgcgggatc gccgccatat tgaccgcgac
    12541 gaaggggccg ttgcgccgct tgccgtagtc gtgcagcgca cgcgcgacga gctccttgcc
    12601 ggtgccggat tcgccggaga tcatcacggt gagatcggtc tgcatcagcc gcgccagcac
    12661 ccggtagatt tcctgcatcg caggcgaacg gccgaccagc ggtatggaat cgaattcgga
    12721 ctcgtcggcg tggttggcca ctcgctcctt cggttccgcc agtgcgcggc cgacgatggc
    12781 gatcagctcc ttcaggtcga acggctttgg cagatactcg taggcgccgc gctcggatgc
    12841 gcggatggcg gtcatgaagg tgttctgcgc gctcatcacg atgaccggaa gattcggccg
    12901 catctttttg atccgcggca acaaatcgaa cgcgttctcg tcgggcatca cgacgtcagt
    12961 gatgaccaga tcgccctcgc cctggctgac ccagcgccac aaggtcgccg cgtttcccgt
    13021 caggcgcacc tcgtatccgg cgcgcgagag agcctggttc agaaccgtac ggatggcggt
    13081 atcatcgtcg gcgacaagta tactacctgc gggcattgtt attcctcatc ttgtgtcctg
    13141 tgaggcatgc gacggcgtcc ccgaagcgtg atcgcgattg ccatggtcga tatgcttggc
    13201 gctgttgaac atcggcagca gcacgcggaa ggtggtcttg cgcggctggg actcgcactc
    13261 gatgatgccg ccatgatcgc cgacaatctt ggccaccagc gcaagaccca gcccgctgcc
    13321 ggtcggtttg gtcgtgacga acggatcgaa caggttcggc aacaagtcct cgggcacgcc
    13381 cgggccgttg tccttgacgc agaattcgag cggcaacgac acccgcgatt ttttccccgg
    13441 caccgacagc cggacgcccg gacggaacgc ggtggtgagt tggatctccg cgtcgctccc
    13501 gagatcgacc acggcctcgg cggcgttctt caccagattc aggaacacct gaatcaattg
    13561 gtcctgattc gcaagcactg gcggcagcga cgggtcgtat tcttccacaa accggatgtt
    13621 gcgggcaaag cccgattgcg ccagccgctt gacgtgatcg agcaccgaat gaatgttgac
    13681 cggaccgcgc gccaccggcc ggtcgtcgcc gaacacttcc atgcgatcga ccagcgtgac
    13741 gatgcggtcc gcctcatcgc agatcagccg cgtcagcatg cgg
//
LOCUS       DATNOI010000244        18449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_601355,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000244 DATNOI010000000
VERSION     DATNOI010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18449
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601355"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="VN968_09220"
     CDS             <1..972
                     /locus_tag="VN968_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXL29453.1"
                     /translation="SVQSIADILFLQGVDIGVVRADSLDYLEKKGIAHDLRKQLTYVT
                     KLYNEEMQVIAPVAVRNLRDLDGKRVSVDLPDGSTFVTALAVFERLGLQANNFAYLEQ
                     RIAMEKLKKGELDAVIVVGGKPYKSVSNFVNDGGRFHLVTVDYDRPLQGDYLPANMTS
                     KDYPNLIKEGESVDTIAVPAVLAAYNWAPNTDRYRKLSLFVDAFFTKFPALQNPPFHP
                     KWKEVSLSAPLPGWQRLPSAKQWLDQHGIEPVARNRFEEFPAQTAAPAAGFKPAPTAA
                     VRQAPAPVAAARPQTPAEQEALFKQFQAWEAERNASAQARPAPQQPR"
     gene            complement(1042..1425)
                     /locus_tag="VN968_09225"
     CDS             complement(1042..1425)
                     /locus_tag="VN968_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HXL29454.1"
                     /translation="MASEHTVGFEPADRLAKDRESVRRRFWLKLKRVVARLPFAEDLL
                     AAYYCAFDKQTPRHVQAALLGAIAYFILPFDFIPDMLPVLGFTDDAAVLATAIRMVAT
                     HITPEHREAARAALKRGIETAEPSE"
     gene            complement(1523..1846)
                     /locus_tag="VN968_09230"
     CDS             complement(1523..1846)
                     /locus_tag="VN968_09230"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HXL29455.1"
                     /translation="MKQEAIMVERLSAEARKSALQGLPGWTETSGREAIARNFTFKDF
                     NEAFGFMCRAALVAEKNDHHPEWRNVYKTVEVILATHDAGGVTALDIDLAKAMNTIAR
                     QLGVA"
     gene            2093..3442
                     /locus_tag="VN968_09235"
     CDS             2093..3442
                     /locus_tag="VN968_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXL29456.1"
                     /translation="MFITLAGILALVGMLAGGYYFAMRPVPLRIAVGPTNSDDVKLVQ
                     ALTQAFSHAHGQVRLHPMQTDGAAASAKALAEGKADLAVIRGDLDVPKNAQAVATLRK
                     NVTVLWVPPAAKGKGKKAGSKITKIAQLSGHRVGVVGRTPANVNLLKVILQQYGVDPN
                     KVDIVQFPAGESADAIRNQKADAYLAAGPVNSKITADAIAASTRDGGTPTFLAIDSAE
                     AIAQNHPMYESSEIPAGAFGGSPARPDEEVKTISFAHHIVARKGLSEATVAAFTRQLF
                     AIRQTVMAEFPLAAKIETPDTDKDAVIPVHPGAAAYVDGEEKTFLDRYSDFIWWGLMG
                     LSAMGSAGAWFAGYLKKEERSNNSSLRERLLNMLGAARRSDSTEELDQMQTEADGILR
                     ETLQCFEHGAIEEGALTAFNIALEQFHNAVADRKALLMSMPQNLQRANVALRASGTM"
     gene            complement(4151..4456)
                     /locus_tag="VN968_09240"
     CDS             complement(4151..4456)
                     /locus_tag="VN968_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29457.1"
                     /translation="MRRSLYIGHRWIGIATCLLFAMWFVSGLVMMYVAFPSLTEGERI
                     AALPDIAWDRVRLTPDRAMAAVGLTQYLRELRLNMLNDEPVYRLLDGDGRRQTVLGG"
     gene            complement(4482..4628)
                     /locus_tag="VN968_09245"
     CDS             complement(4482..4628)
                     /locus_tag="VN968_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29458.1"
                     /translation="MQRKPDAKTTMALRVYNLFDTIYATGGPTTQWLLGMPRTAELSL
                     NVKF"
     regulatory      complement(4877..5087)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5255..6684
                     /locus_tag="VN968_09250"
                     /pseudo
     CDS             5255..6684
                     /locus_tag="VN968_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
     gene            complement(6734..7132)
                     /locus_tag="VN968_09255"
     CDS             complement(6734..7132)
                     /locus_tag="VN968_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL29459.1"
                     /translation="MKPASADGMAALPNDVLIVEDDPLIALDFEDTILGFGVKTVRTA
                     GNVAKALEMIADRAPDFALLDVGLVREKSFAIAERLDALKIPFVFVTGYGAAGTFPAA
                     FARRPRLPKPYSAEELKALLYGRSDGAAKG"
     gene            7304..8074
                     /locus_tag="VN968_09260"
     CDS             7304..8074
                     /locus_tag="VN968_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL29460.1"
                     /translation="MATQRGATPEGIERPFNNLLRRLSASDFALIGPHLAHEEAAAND
                     LLYNPGDDVEIVHFPCGPSLATYLVSNEDGRDVETILVGREGAVGGIVSEGYLPAYTR
                     IMVKFGGPFVRLHVGKLDAAKTKSATLRNVFARYADCMLAQIFQSTACNAIHSIEQRT
                     AKWIISAMERTDDADAVPLTHEQLATMLGVGRSYVSRVIQTFKAEGVLETRRGSIVVR
                     DREALRIRACLCDESVKNHFEEVLRGVYPSDEALGAAK"
     gene            8434..10260
                     /gene="typA"
                     /locus_tag="VN968_09265"
     CDS             8434..10260
                     /gene="typA"
                     /locus_tag="VN968_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HXL29461.1"
                     /translation="MKLRNVAIIAHVDHGKTTLVDRLLQQSGTYRDNQRVVERAMDSN
                     DLERERGITILAKAASVQWKDTRINIVDTPGHADFGGEVERILNMVDGALVLVDAAEG
                     PLPQTKFVVSKALKVGLKPIVVINKVDRPDARPTEVINEVFDLFAALDASEEQLDFPI
                     LYGSAKQGWMAEGPDGPKDAGMQPLFDLILRHVAPPTVEDGPFRMIGTILEANPYLGR
                     IITGRITSGTIKPNQAVKVLGGDGKLIETGRITKIIAFRGIERTPLDEAEAGDIVAIA
                     GLTKGTVADTFCDPSVDTPLQAQPIDPPTVSMSFIVNNSPLAGTEGDKVTSRMIRDRL
                     LREAEGNVALRVTEAADKDSMEVSGRGELQLAILIETMRREGFELSVSRPRVVLKKDE
                     ATGEWQEPVEEVVIDVDEEHSGVVVQKMSERKAEMIEMRPSGGHRLRLVFYAPTRGLI
                     GYQGELLTDTRGTAIMNRLFHGYAPYKGDIQGRRNGVLISNDQGEAVAYAMFKLEDRG
                     PMMITPGAKVYKGMIVGEHTRDNDLEINILKGKQLTNIRTTSKDEAVRLTPPIQMTLE
                     KALAYIEDDELVEVTPKSIRLRKKFLDANDRKRAEKAKEAVA"
     gene            10350..11594
                     /locus_tag="VN968_09270"
     CDS             10350..11594
                     /locus_tag="VN968_09270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL29462.1"
                     /translation="MTLPSEIDVAIIGAGAAGLGAARALENSGLSVVVLEARSRVGGR
                     AHTIMAAPDITFDLGCGWLHSGDRNSFVKIAAQLDFEIDKTRPPWREQSFDIGFPPRE
                     RADFIEALDAFYDRAEEAANSGRDSAASEYLEPGNRWNPMIDAISTYVNGCELDRVSI
                     LDMEAYEDTEINWRVRRGYGALMQAYGAPCPLALNCEVKRIDHSGKRVQIETSQGRLS
                     AGKVIVTIPTNLIASEALRFHPELPAKIDAARGLPLGLANKVMLALDEPEALPKDGNL
                     RGATMRTAMGGYHLRPFGQPCIEGFFGGRFARELEDAGDGALAAQSIDEIAALLGNDF
                     RRKLKPMSESRWAHDRFAQGSYSHALPGHAGKRAVLAAPIDGRLFFAGEATSPNFFST
                     AHGARDSGERAAREVLASVAKG"
     gene            complement(11671..12078)
                     /locus_tag="VN968_09275"
     CDS             complement(11671..12078)
                     /locus_tag="VN968_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HXL29463.1"
                     /translation="MNRWPDDDVILYDGVCVFCSRWIRFVAARDVDRRFRFTAIQSGY
                     GTRLAQAFGIDPDDPDTNAVIHGGIAYLKSDGALTVLSNLPGWGWVRALRFVPKPLRD
                     AVYNLVARNRYRIFGKYEACFVPGAELKARVME"
     gene            complement(12090..12989)
                     /locus_tag="VN968_09280"
     CDS             complement(12090..12989)
                     /locus_tag="VN968_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL29464.1"
                     /translation="MQSSKAFFVLVVLGLVGAQSATAQPRFGAQGAEAEPARMQQWLV
                     PSPDPETAAHALLFRPSGDGPFPLALIAHASTQNVLRRAQMPQPEYRALAAWLVARGF
                     AVLVPERPGHGATGGKYLEDQGGCDEADYSRSGYATADSIKAALGYLREQPFVRQDGT
                     VIVGHSAGAWGALALAGENPKGVSAIIAFAPGRGGHANDLPNQVCAPHTLMQSAAEFG
                     EDALVPVIWLVAANDSYFSPDLSRQLADAFRTGGGRVDFRVLPASGSEGHWLAETEAG
                     VKIAGPELDSALKALTPVAAKKR"
     gene            13268..13777
                     /locus_tag="VN968_09285"
     CDS             13268..13777
                     /locus_tag="VN968_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL29465.1"
                     /translation="MSTTLIEVRPAKAADAAAVASTHDEAWRAAYQGIIPGAELEKLI
                     NRRGPQWWDSAIRKGSRVSVLVFGDKVAGYANYGRNRARSLHFEGEIYELYLRPEFQG
                     LGFGRRLFTSARRDLLQSGLKSMVVWALSDNDPATEFYRALGGRMVARSSEKFGPKSL
                     DKVAFAWTN"
     gene            13877..14407
                     /gene="ppa"
                     /locus_tag="VN968_09290"
     CDS             13877..14407
                     /gene="ppa"
                     /locus_tag="VN968_09290"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HXL29466.1"
                     /translation="MRIEAISIGIDPPRQVNVIIEVPVGGEPIKYEMDKDAGTLVVDR
                     FLYTAMRYPGNYGFIPHTLSGDGDPCDVLVANTRAIVPGAVMSVRPVGVLLMEDEAGG
                     DEKIIAVPSSKLTQRYDKVKTYSDLPDITLHQIQHFFEHYKDLEPGKWVKVLRWGGAE
                     DAHQLILEGIARAKAK"
     gene            14789..15610
                     /locus_tag="VN968_09295"
                     /pseudo
     CDS             14789..15610
                     /locus_tag="VN968_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
     gene            complement(15666..16550)
                     /gene="folD"
                     /locus_tag="VN968_09300"
     CDS             complement(15666..16550)
                     /gene="folD"
                     /locus_tag="VN968_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HXL29467.1"
                     /translation="MTARIIDGKVIASELRARVAAEVARVQRERGITPGLAVVLVGND
                     PASEVYVRSKHKQTQAAGMASFEHVLPADVKQNDLLALIEELNRDSSVHGILVQLPLP
                     KSLDTETIINAIDPAKDVDGLHPSNAGRLAGGLAALSPCTPLGCIILTKSVHASLEGM
                     NAIVIGRSNLVGRPLVQLLLNENATVTIAHSRSRDLAQLCARADLVYAAVGKPEMVRG
                     DWIKPGATVIDVGISRLPGADGKTRLVGDVAFAEALNVAAAITPVPGGVGQMTVACLL
                     VNTLRAACAIHGLPKPAV"
     gene            complement(16547..16897)
                     /locus_tag="VN968_09305"
     CDS             complement(16547..16897)
                     /locus_tag="VN968_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HXL29468.1"
                     /translation="MDPWRYSTQGISVALRVTPRGGRDDIDGLETLADGRMVVKVRVR
                     AIAEGGEANRAVTELLAKALGVPKARVRILSGTTSRLKQIAVDGDPARLGDALRSLTA
                     AKPAKNKGRNKGLR"
     gene            complement(16917..17207)
                     /locus_tag="VN968_09310"
     CDS             complement(16917..17207)
                     /locus_tag="VN968_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HXL29469.1"
                     /translation="MRAVLDIILIVLDLYVWLLIASAILSWLIAFNVVNTRNQFVAAV
                     AEFLYRITEPMLAPIRSFMPNLGGLDISPIILILIIMFIQRVIQYYIYPSVF"
     gene            17401..17476
                     /locus_tag="VN968_09315"
     tRNA            17401..17476
                     /locus_tag="VN968_09315"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17434..17436,aa:Ala,seq:cgc)
     gene            complement(17583..18395)
                     /locus_tag="VN968_09320"
     CDS             complement(17583..18395)
                     /locus_tag="VN968_09320"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXL29470.1"
                     /translation="MTYQHILYEVSEKIATITLHRPDRMNAWTPIMERDVRHAMEAAA
                     ADDDVRVIVLTGSGRAFCAGADMESLKGLDPGDIKRAETVPPFDMNRRPDWQTRYAYY
                     PSIPKPVIGMLNGATAGIGLVHALYCDLRFANDNTVFTTAFSRRGLIAEHGISWMLPR
                     IVGHANALDLLMSARRVQSDEALRIGLVNRLYPPDQLREQTYAYARDLAEFVSPSAIA
                     VIKRQLYDVPFQTLAEATIDANREMVVALRGSDFREGVASFMEKRPPRFTGK"
BASE COUNT         3594 a         5947 c         5688 g         3220 t
ORIGIN      
        1 tccgtgcaaa gcatcgccga catcctgttc ctgcagggcg tcgatatcgg cgtcgtgcgc
       61 gccgattcgc tcgactatct cgagaagaag ggcattgccc acgacctcag gaagcagttg
      121 acctacgtca ccaagctgta caacgaggaa atgcaggtca tcgccccggt ggcggtccgc
      181 aacctgcggg acctcgacgg caagagagtc agcgtcgacc tccccgatgg cagcactttc
      241 gttaccgcac tggccgtgtt cgagcggctc ggcctccagg cgaacaattt cgcctatctc
      301 gagcagcgca tcgcgatgga gaaattgaag aagggcgagc tcgacgccgt gatcgtcgtc
      361 ggtggcaagc cgtacaaatc cgtcagcaac ttcgtcaacg acggaggccg gttccacctg
      421 gttacggtcg attacgacag gccgctgcag ggcgactatc tgcccgcgaa catgacgtcg
      481 aaggattatc cgaacctgat caaggaaggg gaatcggttg acaccatcgc ggttcccgcg
      541 gtgctggccg cctataattg ggcacccaac accgaccgct atcggaagct ctccctgttc
      601 gtggatgctt tcttcacgaa attcccggcc ctccaaaatc cgccgttcca tccgaagtgg
      661 aaggaggtgt cgctttcggc tccgcttccc ggttggcagc gcttgcccag cgccaagcag
      721 tggctggatc agcatggcat cgaacccgtc gcgcgcaacc ggttcgagga atttccggcg
      781 cagaccgccg ctcctgcggc aggctttaag ccggccccta ctgccgcagt gaggcaggcc
      841 ccagcccctg tggcggcggc aaggccgcag acgccggccg agcaggaagc cttgttcaag
      901 caattccagg catgggaagc cgagcggaat gcctcggcgc aagcccgccc cgcaccgcaa
      961 cagccgcgat aggttctatc caggagtgat cccgagtcgg acgaatattc cggctcgccg
     1021 atcgctctcg atgaactacc gtcattccga tggttcagcc gtctcgatcc cccgcttcaa
     1081 cgcggcgcgg gcggcttcgc gatgctccgg cgtgatgtgg gtggcgacca tccggatcgc
     1141 ggtcgccagt accgcggcgt cgtcggtgaa gccgagcacg ggcagcatgt ccggaatgaa
     1201 atcgaacggc aggatgaaat aggcgatggc gccgagcagt gccgcctgca catggcgcgg
     1261 ggtctgcttg tcgaacgcgc aataatacgc cgcgagcaag tcctcggcga acgggagcct
     1321 ggcgacgacc cgcttcagct tgagccagaa gcgcctgcgc acgctttcgc ggtctttcgc
     1381 cagccggtcg gccggctcaa atcccacggt atgttcggat gccatcattc aaggctccct
     1441 cgagatgcgc ggtaaatcgc ctcggctgcc gcctccagat ttggggacgc ggcggcgccg
     1501 acgcaagatt gcgcatcgct ggtcaagcga cgccgagctg ccttgcgatg gtattcatcg
     1561 cctttgcaag atcgatgtcg agggcggtga cgccgccggc gtcatgggtc gccagaatca
     1621 cctccaccgt cttgtagaca ttgcgccatt cggggtgatg atcgttcttt tcagccacca
     1681 atgcggcgcg gcacatgaac ccgaatgctt cgttgaaatc cttgaaggta aagtttcgcg
     1741 caattgcctc gcgtcccgag gtctcggtcc accccggcaa accctgcagc gccgattttc
     1801 gcgcctccgc cgacagccgc tccaccatga tcgcctcctg tttcacacca gctctaccct
     1861 aacaccaacg gcaccccttg ggatccacac ctacatcgag ccggcaatgc cctgccacta
     1921 accatgacgc agatgtaacc tcgcgccggc gggaaatttg ctacaaggcc ggtctaaccg
     1981 cattggcaag atgaaattaa gcgtcaaacg attgtttgcc cgatcgatga ccgagggcct
     2041 cgaccttgtc gggccgaccc tgccggcgcc gcgttcggcg aaacagaaga cgatgttcat
     2101 caccctggcg gggatcctcg ccctggtggg catgctcgcg ggcggttact attttgcgat
     2161 gcggccggtg cccctgcgga ttgcagtcgg tccaaccaac agcgacgacg tcaaactggt
     2221 tcaggcgctg acccaggcct tttcccatgc ccacggtcag gttcgtttac acccgatgca
     2281 gaccgacggc gccgccgcga gtgcaaaggc gcttgccgaa ggcaaggccg atctcgcggt
     2341 catccgcggc gatctcgatg tgccgaaaaa tgcccaggcc gtggcaacgc tgcgcaagaa
     2401 cgtcaccgtg ctatgggtgc ctccggccgc caagggcaaa ggcaagaaag ccggttcgaa
     2461 gatcacgaag atcgcgcagc tctccggaca ccgcgtcggt gtggtcggac gcactccggc
     2521 caatgtcaat ctgctcaagg tgatcctgca gcaatacggt gtcgatccga acaaggtcga
     2581 tatcgttcag ttcccggccg gcgagtccgc ggacgccatc cgcaaccaga aggcggacgc
     2641 ctatcttgcc gccggaccgg tcaacagcaa gattaccgct gatgcaatcg cggcatcgac
     2701 ccgcgatggc ggcacgccga cctttctcgc gatcgattcg gccgaggcga tcgcgcaaaa
     2761 ccacccgatg tacgaatcct ccgaaatccc cgccggggcg ttcggcggct cgcccgccag
     2821 gccggacgaa gaggtgaaga cgatcagctt cgcccatcat atcgttgcac ggaagggtct
     2881 gtcggaagcg accgtcgccg cctttacccg gcagctgttt gcgatcaggc agacggtgat
     2941 ggcggagttc ccgctggcgg cgaagatcga gacacccgac accgacaagg acgccgtgat
     3001 ccccgtgcat ccgggtgcgg cggcttacgt cgacggcgag gaaaaaacct tcctggaccg
     3061 ctacagcgat ttcatctggt gggggctgat gggcctgtcc gcgatgggct cggccggcgc
     3121 atggttcgcg ggctatctga aaaaggagga gcgcagcaac aacagttcat tgcgcgaacg
     3181 gttgctgaac atgcttggcg ccgcgcgccg cagcgattcc accgaagagc tcgaccagat
     3241 gcagaccgag gcggacggta tccttcgcga gacgctgcag tgcttcgagc acggcgccat
     3301 cgaggaaggg gcgttgaccg ctttcaatat cgcacttgag caattccaca acgccgtggc
     3361 cgaccgcaag gcgttgctga tgagcatgcc gcagaacctg caaagggcca acgtcgcgct
     3421 gcgcgccagc ggcacgatgt agaaatcagc gcgccagccg atcacgatcc cgctcaccga
     3481 aatcgtcaac cccaatagcg acagcagcca caccacgata tcccacgccg gacgccaggc
     3541 cagcagccat gggaaattgg gcgcgtcatg gccggcgtaa cgcagcgcca tctcgcgtga
     3601 cggtccacgg ccggcgaaga attcgccggg atcgagcgac agccagccgc tgaatatcca
     3661 ggtcagcacg aacatgcccg caaagaatcc cgtgacatga tgccacgcca tccagccgcg
     3721 atagggcgtg atgctgccgc cggaatagcg ccgccgcagc cgcacccgca aacccccgat
     3781 ccagatgccg ctcaccgccg ccagcatgca gatgcccgaa atccacaaca cgacctgccg
     3841 ccacagcgga ccgtcctggc gcagcagggt gggatagatc cagtgcggta tagatccgag
     3901 ccagttccag atgcgctcgg tccgcgtggt atcgagcacg atctcaccgc tacgggagga
     3961 aacgtagagc tccgtgccat cggcgtcgcc gagcgagacg agaaagaact gccgcgcggg
     4021 atcgaatcgc gcggtgacgc tccattggtc gcgatcgacg gtgccggccg attgcacacc
     4081 cgccgcagcg ggatgtcgcc tcaccaccgc cagcgcctgt tcaagcgaga cgctgtcgat
     4141 gacgcggcca tcaaccgccg agaaccgttt gccgccggcc atcgccgtcc agcaaccgat
     4201 agaccggctc atcgttcagc atgttcagcc gcagttcgcg cagatactgc gtcagcccga
     4261 cggccgccat cgcgcgatcc ggcgtgagcc gtaccctgtc ccaggcgata tcgggaagcg
     4321 ccgcaatgcg ctccccctcg gtgaggcttg gaaacgccac atacatcatg acaaggccgg
     4381 acacgaacca catcgcgaac agcaggcagg tggcgatgcc gatccatcgg tggccgatat
     4441 acaacgaccg ccgcaaccgc cggatcaccg cgcgcgcacg gtcagaattt tacattgagc
     4501 gacaattccg cggtccgcgg catgccgagc agccactgcg tggtcggacc gcctgtcgca
     4561 taaatcgtgt caaacagatt gtagacccgc agcgccatcg tcgtcttggc atccggcttc
     4621 cgttgcacgc cggcattgac gacggtgtat cgagatcgcg gctcacggcc gcgccctgcg
     4681 tccaggcatc gaccggtaag aagatggaga gcgcaaagga gtacagcaaa gacgcgcgtc
     4741 gggacgcacg aaggcagacg gaggtcatca aatggactcg cggttaacga ggcacgtcac
     4801 cgcgaacgaa gtctccccac agcccggttc gggcgaggtt cggctaccac caggacaccc
     4861 cggcccaatg tgcttgcgtg tgaccacgac aggcggcagg tctcctggct cgccggtcgc
     4921 tgcctttcac cgccttccca gacacaagtg cccagtggct aatggcgaaa ggctcgccgc
     4981 ttacagttgc gggggcagcc gcggaatcgg gaccctgttc gaagaactgg ccctcaccgc
     5041 attccctttt gatcccccgg ggggaaaccg tcaccacgac gttatgttga aaccgcaaaa
     5101 gctgtcaacg gcgtcggttt tgatagcgat gccgggatca ttcggacggg tgttcgaccg
     5161 atcccaattc caacgccaaa cctgtcatcg ccgcgtcaac caacttccgt aagtgggggc
     5221 aatttccttc gagatcttcg atcaggagct cgctatggcc attcgactgt cccgccgccg
     5281 ttttctctcg accaccgcgg ctaccggctt cgccacgctg gcgatgccct atctcagccg
     5341 cgccgccgac cggccgcaaa tcacccatgg cgtacaatcc ggcgacgttg gcgccgacgg
     5401 cggggtggtg tgggcgcgcg cggaccggcc gtcgcaaatg atggtcgagg tcgccacaac
     5461 ggaatcgttt ggcaatgcga gggctttgcc accgatcgcg gcccttcccg agagcgattt
     5521 caccgccaaa atgctgctgg aaaatctccc ggccggacag gacatttttt atcgggtcaa
     5581 gttccgcgac ctgtcgcata ccgacgtgtc gagcgaaccg gtagtcggcc gcttccgcac
     5641 cgcgccggcc gaccggcgcg atgtcagctt cgtctggggc ggcgatgtcg ccgggcaagg
     5701 ctggggcatc aatcccgatg acggcggcat gctgacctac gccactatgc gcaagcacaa
     5761 gccggatttc ctgctccatt ccggcgacac catctatgcc gacggcatca ttgccgccga
     5821 ggtgaaatta cccgacggca aggtctggaa aaacgtaacg atcccggaaa gggccaaggt
     5881 ggccgaaacg ctcgatgaat tccgcgccgc gcataagtac aatttcctcg acgacaatgt
     5941 gcgtgccttc aatgccgagg tcccgatctt cgtgcagtgg gacgaccacg aagtcaccaa
     6001 caactggtcg gcgtcgaagc agcttcccgc cgcctacaag gagcgcgaca tcatcttgct
     6061 tgccgcgcgc gccggccgcg cgttccacga gatgtacccg atgcgcgaaa gcatcgtcga
     6121 gccgggccgg gtctatcgca cgctcaatta cggcccgcat ctcgatgtgt tcatgctgga
     6181 cgagcgcagc tatcgcggcc cgaacggccc taacctgcaa accgtctacg gtcccgacag
     6241 ctacttcatc ggccccgacc agctgttgtg gctgaaacgc gcattattgg ccgcgagctc
     6301 gaaatcgccg agatcctgcg cttcatcaag acgtcgggcg tcaccaacac ggtatggctg
     6361 acggcggatg tgcattacac cgccgcgcat tactacaatc cggacaaggc gcaatttcag
     6421 gacttcgagc cgttctggga atttgtctca ggccccctgc acgccggcac gttcggaccg
     6481 aacgaactcg acaacacctt cggtcccgag gtgaagttca tcaaggcgcc gggatcggac
     6541 aagcagaacc tgccgccatc ggccggcatg cagttcttcg gccatgtcaa gatcgatggc
     6601 gcatccgggc agatgacggt gaccctgcgc gaccgcgccg atgtggcgct gtggtctacc
     6661 acgctcgatc cgaagcccgg gtagccaacc ggccgttgat gtcgctcgcg gcgcgcgggg
     6721 caaatgtcgc cggtcagccc ttcgctgcgc cgtctgaccg gccatacaac aacgccttca
     6781 gctcttcggc agaatagggt ttcggcagcc ttggcctgcg cgcgaacgct gccggaaacg
     6841 tgccggcggc gccgtatccg gtgacgaaca caaaaggaat ttttagcgcg tccagccgct
     6901 cggcaatggc aaaactcttt tcccgaacca gcccgacgtc gagcagagca aaatccgggg
     6961 cgcggtcggc gatcatctcg agcgccttgg ccacattgcc cgcggtccgc accgtcttca
     7021 ccccgaatcc aagaatcgtg tcctcgaagt cgagcgcgat gagcggatca tcctcgacaa
     7081 tcaacacgtc attgggcaag gcggccatgc cgtcggcaga ggcgggcttc atgatctcgt
     7141 cgagcaagag gatactgacc caggcctgac gcctatgttg gcgcatcgcc cggtttccac
     7201 accggacccc accacaccgc gctttccctg tctgtgcact tgtgcacaca tggggttatg
     7261 tgaaggcggt attggtgggg ccagggagga gcgaggttcg aggatggcga cgcagcgcgg
     7321 cgcaacaccc gagggcattg aacggccctt caacaacctg ttgcggcggc tcagcgccag
     7381 cgactttgcg ctgattgggc cacatctcgc gcacgaagaa gccgcggcaa acgacctgct
     7441 ctataatccc ggcgacgatg tcgagatcgt ccactttccg tgcggaccga gtcttgcgac
     7501 atatctcgtc tccaacgaag acggccgcga cgtcgaaacc attctggtcg gccgcgaggg
     7561 cgcggttggc gggattgtca gcgagggcta tctgccggcc tatacccgta tcatggtcaa
     7621 gttcggcggg ccgttcgtac gcctgcatgt cggaaagctg gatgcggcga agacgaagtc
     7681 ggccacgttg cgcaacgtgt tcgcacgcta tgccgactgc atgctggccc agatcttcca
     7741 gtcgacggcc tgcaacgcga tccattcgat cgagcagcgc acggcgaaat ggatcatctc
     7801 ggcgatggag cgtaccgacg acgccgacgc cgtgccgctg acgcatgagc aacttgccac
     7861 catgctgggg gttggccgca gctatgtcag ccgcgtgatc cagacgttca aggcggaagg
     7921 ggtgctggag acccgccgcg gctcgatcgt ggtgcgcgac cgcgaggccc tgcggatccg
     7981 ggcctgcctc tgcgacgaat ccgtcaagaa tcattttgag gaagtgctgc gcggggtata
     8041 tccgagcgat gaggcccttg gcgccgccaa atagcggcgc caacgccgcc ttgctggcca
     8101 aatcacgcgg cgcaatagcg ctgatcctga tcaggcacgc gctgcgcgga ctgcggcgtt
     8161 aactgttttg cgcgggaagc accgggcaac gcccgaagtg ctggaaatgc ctatgaattt
     8221 tgcctgatgc gatgaggttt cccgttgttt ttcgccgatt catgactata ttgcgctgca
     8281 acgagtaaag gtttgcccgg tccttaggcc gggcttcctt tttgggcgtc atgcgccctt
     8341 cgaattccag gttttgagcg tgatccggaa aagtgggtcc cggttttccg gtgagatcgt
     8401 gctccagcca ttgcctgacc cagaagaaga gctatgaaac ttcgcaacgt cgccattatc
     8461 gcccacgtcg accacggcaa gaccacgctg gtcgatcgcc tgctgcagca atccggcacc
     8521 taccgcgaca accagcgcgt ggttgagcgc gcgatggact ccaacgacct ggagcgcgag
     8581 cgcggcatca ccattctcgc caaggccgcc tcggtgcagt ggaaggacac ccgcatcaac
     8641 atcgtcgaca cgccgggcca cgccgatttc ggtggcgagg tcgagcgcat cctcaacatg
     8701 gtggacggcg cgctggtgct ggtggacgcc gccgaaggcc cgctgccgca aaccaaattc
     8761 gtggtgtcga aggcgctgaa ggtcgggttg aagccgatcg tcgtcatcaa caaggtcgac
     8821 cgccccgacg cgcggccgac cgaagtcatc aatgaagtat tcgacctgtt cgcggcgctc
     8881 gatgcgtccg aggagcagct cgactttccg attttgtacg gatcggccaa acagggctgg
     8941 atggcggaag gccctgatgg tccgaaggac gccggcatgc agccgctgtt cgacctgatc
     9001 ctgcgccatg tcgcgccgcc gacggtcgag gacggcccgt tccgcatgat cggcaccatt
     9061 ctggaggcca atccctatct cggccgcatc atcaccggcc gcatcacctc cggcaccatc
     9121 aagccgaacc aggccgtcaa ggtgctgggc ggcgacggca agctgatcga gaccgggcgg
     9181 atcacaaaaa tcatcgcgtt ccgcggcatc gagcgcacgc cgctggatga ggccgaagcc
     9241 ggcgacatcg tcgccattgc cggcctcacc aagggcaccg tcgccgatac cttctgcgat
     9301 ccttcggtcg acacgccgct tcaggcgcag ccgatcgatc ccccgacggt gtcgatgtcg
     9361 ttcatcgtca acaactcgcc gctggccggc accgaaggcg acaaggtcac cagccgcatg
     9421 atccgcgacc gcctgctgcg cgaggccgaa ggcaatgtcg cgctgcgggt gaccgaagcc
     9481 gccgacaagg actccatgga agtctcgggc cgcggcgaat tgcaactcgc gatcctgatc
     9541 gagaccatgc gccgcgaagg ctttgaactg tcggtgtcgc gcccgcgcgt tgtcctcaag
     9601 aaggacgagg cgaccggcga gtggcaggag ccggtcgaag aggtcgtgat cgacgtcgac
     9661 gaggagcatt ctggcgtggt cgtgcagaag atgagcgaac gcaaggccga gatgatcgag
     9721 atgcgtccct cgggcggcca ccggctgcgg ctggtgttct acgcgccgac ccgaggcctg
     9781 atcggctatc agggcgaatt gctgaccgac acccgcggca ccgcgatcat gaaccgcctg
     9841 ttccacggct atgcgcccta caagggcgac atccagggcc gccgcaacgg cgtcctgatc
     9901 tccaacgatc agggcgaggc cgtagcctat gcgatgttca agctggagga ccgcggcccg
     9961 atgatgatca cgcccggcgc caaggtctac aagggcatga tcgtcggcga gcatacccgc
    10021 gacaacgacc tcgagatcaa catcctgaag ggcaagcaac tcaccaacat ccgcaccacc
    10081 tcgaaggatg aagcggtgcg cctgaccccg ccgatccaga tgaccctcga aaaggcgctg
    10141 gcctatatcg aggacgacga actggtcgag gtgaccccga aatcgatccg gctgcgcaaa
    10201 aaattcctcg acgccaacga ccgcaagcgc gcggaaaagg ccaaggaagc ggtggcctaa
    10261 gtgggcgaat agggagtggc gaatggcgag tagacgaacc atataagtga agttctattc
    10321 gctcttcgcc actcaccatt cgccctgtca tgaccctccc ctccgaaatc gatgtcgcca
    10381 tcatcggtgc gggcgccgcc ggccttgggg ctgcgcgggc gctggaaaac tccggcctct
    10441 cggtggtcgt gctggaagcg cggagccgcg tcggcgggcg tgcccacacc attatggcgg
    10501 cgcccgatat caccttcgac ctcggctgcg gctggctgca ttcgggcgac cggaattcgt
    10561 tcgtcaaaat cgccgcgcaa cttgatttcg aaatcgacaa aacccgcccg ccatggcgcg
    10621 agcagagttt tgacatcggg tttccgccaa gggagcgcgc cgacttcatc gaagccctcg
    10681 atgcgttcta tgaccgcgcc gaagaggccg cgaatagcgg ccgcgacagt gccgcgagcg
    10741 aatatctcga gcccggcaat cgctggaatc cgatgatcga tgcgatctcg acctacgtca
    10801 acggctgcga actcgaccgc gtctcgatcc tcgacatgga ggcctacgag gacaccgaga
    10861 tcaactggcg ggtacggcgc ggctacggcg cgctgatgca ggcctatggc gcgccctgcc
    10921 cgctcgcgct caattgcgag gtgaagcgca tcgatcattc cggcaagcgg gtacagatcg
    10981 agacatcgca aggccggctt tcggccggca aggtgatcgt caccatcccc accaacctga
    11041 tcgccagtga ggcgcttcga tttcacccgg agctgccggc aaaaattgac gccgcgcgcg
    11101 gcctgccgct tggcctcgcc aacaaggtga tgctggcgct cgacgaaccc gaggcgctgc
    11161 cgaaggacgg caatctgcgc ggcgccacca tgcgcaccgc aatgggtggt tatcatctgc
    11221 ggccgttcgg ccagccctgc atcgaaggtt ttttcggcgg ccgctttgcg cgggaattgg
    11281 aagatgccgg cgacggcgca ctcgcggcac aaagcatcga cgagatcgca gcgctgctcg
    11341 gcaatgattt ccgccgcaaa ctaaaaccga tgtcggagtc gcgctgggcg cacgaccggt
    11401 tcgcccaagg ctcctactcg cacgccctgc ccggccatgc ggggaagcgc gcggtgctgg
    11461 cggcgccgat tgatgggcgt ttgttttttg ccggagaggc gacctcgccg aactttttct
    11521 ccacggcaca tggcgcgcgg gatagtgggg agagggcggc gagggaggtg ctggcttcgg
    11581 tcgcgaaggg atgagtgtcg atctctggat gttcgcggaa cctgtcatcg ggtggcgctt
    11641 tgcgccgacc caccgatgac gcaatttccc ttactccatc actctcgcct tcagctcggc
    11701 acctggaaca aagcacgctt cgtacttccc gaaaatccgg tagcggttgc gggcgacgag
    11761 attgtagacc gcgtcacgta gcggcttcgg cacgaagcgc aaggcgcgca cccagcccca
    11821 gcccggcaaa ttcgacagca ccgtcaatgc cccgtcggat tttaagtagg caataccgcc
    11881 atgaatgaca gcattggtgt caggatcatc cgggtcgatg ccgaacgcct gcgccagccg
    11941 cgtgccgtag ccggactgga tcgcggtgaa gcggaaccgg cgatcgacat cgcgcgcggc
    12001 gacgaagcgg atccagcgcg agcaaaacac gcagacgccg tcgtagagga tcacgtcgtc
    12061 gtccggccac ctgttcaccg tgcccagcgt catcgctttt tcgccgcgac cggcgtgagc
    12121 gccttcagcg cgctgtccag ttccggtccg gcaatcttga cgccggcctc ggtctccgcc
    12181 agccagtggc cctcgctgcc ggatgccggc agcacgcgaa agtccaccct tccgccgccg
    12241 gtgcggaacg catcggccag ctgccgcgac agatcgggtg agaaatagct gtcattggcc
    12301 gccaccagcc agatcaccgg caccagcgca tcctcgccga attcggccgc ggattgcatc
    12361 agcgtgtgcg gcgcgcagac ctggttcgga aggtcgttgg cgtggccgcc gcgccccggc
    12421 gcgaaggcga tgatggcgga gacgcctttc ggattctcgc cggccagcgc cagtgctccc
    12481 caggcgccag ccgagtgccc gacaatgacc gtgccgtcct gccggacgaa aggctgttcg
    12541 cgaagatagc cgagcgcggc tttaattgag tctgctgtcg catagccgga gcgggaatag
    12601 tcggcttcgt cacagccgcc ctggtcctcg agatattttc cgccggtcgc gccgtgcccc
    12661 ggacgttccg gcaccagcac ggcaaagccg cgcgcgacta gccaggccgc cagcgcgcgg
    12721 tattcgggct gcggcatttg ggcgcgccgc agcacgtttt gcgtcgaggc gtgggcaatc
    12781 agcgccagcg gaaacggccc atcgcccgag gggcggaaca acagtgcgtg cgccgcggtt
    12841 tcaggatccg gcgatggcac cagccattgc tgcatgcggg caggctcggc ttcagcacct
    12901 tgcgcgccga accgcggctg cgccgtcgcg ctctgcgcac cgacgagtcc aagcacaacc
    12961 agtacgaaaa acgccttgct cgattgcatg aattgccctg acgccaaccg gaaaacccga
    13021 ctcggattcg cgcggcacaa tacctatggc gaatcaaatc cagtgcattt ttccgtgaac
    13081 gcggcctgcc aaaacgtttg gtccggttgt catagtccgc caaccccgat cgaaatgtcg
    13141 cttaaaacac gtccctgtcc tctttcggta caaccggacg gtaaaactga tgcccaaaat
    13201 ccacaggtta accgataatt aacgatggac cttgatgcgg acgcgctgaa ttgctttgat
    13261 tagaaccatg agcacaaccc tgatcgaggt tcggccggcc aaagctgcgg atgcggccgc
    13321 ggtggcgtcc acccatgacg aagcctggcg cgcggcctat cagggcatca ttcctggtgc
    13381 cgagctggag aagctgatca accgccgcgg cccgcaatgg tgggacagcg cgatccgcaa
    13441 gggcagccgc gtcagcgtgc tggtgttcgg cgacaaggtc gcaggctacg ccaattacgg
    13501 ccgcaaccgc gcgcgcagcc tgcacttcga aggcgagatt tatgagctgt acctgcggcc
    13561 ggaatttcag ggccttggct ttggccgccg gctgttcacc tccgcgcggc gcgacctgct
    13621 gcagagcggc ctgaagagca tggtggtctg ggcgctctcc gacaacgacc ccgcgacgga
    13681 attttaccgc gcccttggcg gccgcatggt cgcgcgttcg tcagagaaat tcgggcccaa
    13741 gtcgctcgac aaggtggcgt tcgcctggac taattgagtt cttcggattc gctgactttc
    13801 gcagcacgct actcgcctcg ggccgagttg ccgcccgccg atccaaagcc tgatctcgga
    13861 gctatgcgga gcatccatgc gcattgaggc catctcgatt gggatcgatc cgccgcgtca
    13921 ggtcaacgtc atcatcgaag tgcccgtggg cggcgagccg atcaaatacg agatggacaa
    13981 ggacgccggc acgctggtgg tcgaccggtt tctctacacc gcgatgcgct atcccggaaa
    14041 ttacgggttc attccgcata cgctctcggg cgatggcgat ccttgcgacg tgctggtcgc
    14101 caacacccgc gcgatcgtcc cgggcgccgt gatgagcgtg cgccccgtgg gcgtgctgct
    14161 gatggaagac gaagccggcg gcgacgagaa aatcatcgcg gtgccgtcgt cgaagctgac
    14221 ccagcgctat gacaaggtga aaacctacag cgacctgccc gacatcacgc tgcaccagat
    14281 ccagcacttc ttcgagcact acaaggatct cgaacccggc aaatgggtga aagtgctgcg
    14341 ttggggcggc gccgaggatg cgcatcaatt gattctcgaa ggtatcgcgc gcgccaaggc
    14401 aaagtagccg ttgatgagac atgagtcgcg acgcgcacgc tctgctctct ccccccggcg
    14461 accggggtct gccccggttg cgcgtcttaa tcttgcgcag gtcgggccgg cccgacttgc
    14521 gttgggggag agccagagag gagggtgccg cggactccgg tgcccgtggc tcaccccctc
    14581 cctcccccac gaggggggag ggaacgaagc aagcgagcaa atcgaagctt ctcccaactt
    14641 cggcggacca tgcgccggct cataagaatc atccgcaaca tcctgctgct cgtgatcgct
    14701 gcgatggtga tcctcgccgg cgtgctgacg ttcaatgtgg ttacgcaccg ctcgcggcaa
    14761 gatcgaggtc tccgcggtac cccgcgcgct cgatgaaggc ctgttgatcg cggaaggcct
    14821 cattaaaggc ctcggccggc cagccgcatt gatcggcgtc gccgaaaaag gctatgccac
    14881 gctggttttg acggcgcatg cgacgcccgg tcattcgtcg atgccgccgc gcgataccgc
    14941 gatcggcatg atgagcgcag cgttggcgcg gctcgaagac catcggctgc cgatgcagat
    15001 tcgcggcgcc gtctcggaaa tgttcgacac actggcgccg gaaatgagcg gcttcaaccg
    15061 cgtcgcgctg tccaatctct ggctgttcaa gctgttatta ttgcgcgaat tcgaaaagag
    15121 cggaccgagc gaagcgacag tccgcaccac caccgcgctg acgatcttca atgccggcga
    15181 caaggacaac gtgctgcccg gcaatgccga ggccacggtc aacttccgcc tgatcccggg
    15241 cgatacgcag gcgagcgtga ccgatcatgt tcgtcctacc gtcaataacg accgtatctc
    15301 gatcgaaccg tttcccggca acaccgatcc gccgccggtg acgggcaccg cgagcccatc
    15361 ctaccgcatg ctcaaccgga ccatccggga gatttttccc gacgtcgtgg tcgcgcccgg
    15421 gctgatggtg gcggcgaccg actcgcgcca ctacgccggg atcgccgaca acatcttccg
    15481 gttttcgccg gtgcgcgcca actcggaaga tctgaaacgg tttcacggca ccaacgagcg
    15541 cctcagcatc gaaggctatg ccgatatgat ccggttctat cgacggctga tcgagaatac
    15601 ggcagggtga cgctttcttc cctccccgca agtgcccgca agcaggagag ggggaagagt
    15661 ccaagtcaca ccgccggctt gggcaacccg tgaatggcgc aggcggcgcg cagcgtgttg
    15721 accagcagac acgccaccgt catctgaccg acgccgccgg gcaccggtgt gatcgcggcc
    15781 gcgacattca gcgcctcggc aaacgcgaca tcgccgacca gacgggtctt gccgtcagcg
    15841 cccggcagcc ggctgatacc gacatcgatc accgtggcgc ccggcttgat ccaatcgccg
    15901 cgcaccattt cgggcttgcc gacggcggcg taaaccagat cggcgcgggc gcagagttgc
    15961 gcgagatcgc gcgagcgcga atgggcgatc gttaccgtgg cgttttcgtt gagcagcaat
    16021 tgcaccagcg gcctgccgac cagattggaa cggccgatga cgatggcgtt catgccttcg
    16081 agcgacgcat gcacgctctt ggtgaggata atgcacccca acggcgtgca cggcgacagc
    16141 gcggccaatc cgccggccag gcgtccggcg ttgctgggat gcagcccgtc gacatccttg
    16201 gccggatcga tggcgttgat gatggtttcg gtatcaagcg atttcggcag cggcagctgc
    16261 accaggatgc cgtgcacgct cgaatcgcga ttgagctctt cgatcagcgc cagcaaatca
    16321 ttctgcttaa cgtcggccgg caacacgtgc tcgaacgacg ccatgccggc ggcttgcgtt
    16381 tgcttgtgct tgctgcggac atagacctcg ctggcggggt cattgccgac cagcaccacc
    16441 gcgaggccgg gcgtaatccc ccgctcgcgc tgtacccgcg cgacttcagc ggcaacacgg
    16501 gcgcgaagct ctgatgcaat gacctttcca tcgatgatcc gggccgtcat ctcagtcctt
    16561 tgtttctgcc tttgtttttt gccggctttg cggcggtcag gctccgcagg gcgtcgccga
    16621 gcctggcggg atcgccatcg acggcgattt gtttcaggcg cgatgtcgtg ccggacagaa
    16681 tccgcaccct ggccttgggc acgccgagcg cttttgccaa cagctccgtg accgcgcgat
    16741 tggcctcgcc gccttcggcg atggcgcgga cacgcacttt taccaccatg cggccatcgg
    16801 ccagcgtctc aagtccgtcg atgtcgtcgc ggccgccgcg cggcgtgacc cgcagcgcga
    16861 cgctgatgcc ttgggtggaa taacgccaag gatccataaa gcatccaggc cggcgatcag
    16921 aatacgctcg gataaatgta gtattggatc acccgctgaa tgaacatgat gatcaggatc
    16981 aggatgatcg gcgagatatc gagaccgccg agattcggca tgaacgagcg aatcggcgcc
    17041 agcatcggct cggtgatccg gtacagaaac tccgcgaccg ccgcgacaaa ctggttacgc
    17101 gtgttcacga cattgaaggc gatcagccag gacaggatgg cggaggcaat cagcagccag
    17161 acatagagat cgagaacgat aagaatgatg tcgagaacgg cacgcatggg tgggcttact
    17221 cgcggggttg gtcgcggatt cgacttcttg ctagataccg gttcatcccc ttgcaaacaa
    17281 gggaagttcc gggcaaatgc cgccgaaacg gcgttttcgc cgttcttgac ttggccttgg
    17341 tgcggattgt aaatggcgcc gattgtctgc cgtcgtggtt aacgtcgggg cagtcgcgat
    17401 ggggccatag ctcagctggg agagcgcgtc gttcgcaatg acgaggtcgg cggttcgatc
    17461 ccgcctggct ccaccacgct tcgcccttcg ggctacgcgt ggcgcagccg cgcaagaccg
    17521 aaggcgaagc gtgcccggca tagcccgaag ggcgacgacg ggctctggca attcacctgg
    17581 tattacttcc ccgtaaaccg cggcggccgc ttttccatga aactcgccac gccttcgcga
    17641 aaatcgctgc cgcgcagcgc caccaccatc tcccggttgg cgtcgatggt cgcttcggcc
    17701 aaggtctgga acggcacatc gtaaagctgc cgcttgatga cggcgatcgc gctcggcgaa
    17761 acgaactcgg ccagatcgcg cgcataggca taggtctgct cgcgcaactg atcaggcgga
    17821 tacagacggt tgacgagccc gatgcgcagt gcctcgtcgc tttgcactcg gcgcgcagac
    17881 atcaagagat caagcgcatt ggcgtgaccg acgatgcgcg gcagcatcca gctgatgcca
    17941 tgctcggcga tcagcccgcg gcgcgagaac gcggtggtga acacggtgtt gtcgttggcg
    18001 aaacgaagat cgcaatacag cgcgtgcacg agcccgatgc cggcggtggc gccgttcagc
    18061 atgccgatga cgggtttggg tatcgacgga tagtaggcat agcgcgtctg ccagtccggc
    18121 ctgcggttca tatcgaacgg cggcacggtt tcggcgcgct tgatatcgcc gggatcgagc
    18181 cccttcaagc tttccatgtc cgcgccggcg cagaatgcgc gccccgaacc ggtgagcacg
    18241 atgacgcgca catcgtcatc cgcggccgcg gcttccatgg catggcgcac gtcgcgctcc
    18301 atgatcggcg tccacgcatt catgcggtcg ggccggtgaa gcgtgatggt cgcgatcttc
    18361 tcgctcactt catagagaat atgctgatag gtcaccgcga tctccccgtg ttcatcattg
    18421 tccttgcgac aaattgctat tgttgatgg
//
LOCUS       DATNOI010000245         3325 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1132096,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000245 DATNOI010000000
VERSION     DATNOI010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132096"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1091
                     /locus_tag="VN968_09325"
     CDS             282..1091
                     /locus_tag="VN968_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL29471.1"
                     /translation="MQTLHVNGYDMAYLEVGNGPALVCVHGSLCDFRIWSCVLGPLSR
                     RHRVITVSLRHFFPEHWDGVGDTYSIAQHTADVIAFIEKLDTGPVDLMGHSRGGHISF
                     RVAQQRPDLLRRLILAEPGGELDATLDPAFKPGPSPLAARIAATAEKIAAGDIDGGLM
                     LFMDALEGPGAWKRLPATPKQLLRDNAMTLLGQTGDKRPPFSLADAEAIKAPTLFIGG
                     ANTKGALPQVLHTLAAHVSNSRTAMIPGATHPMFEQAPQKFCEIVLEFLGA"
     gene            complement(1111..1803)
                     /locus_tag="VN968_09330"
     CDS             complement(1111..1803)
                     /locus_tag="VN968_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29472.1"
                     /translation="MIPDYLERLARELSFDRSLARNVRQEVEDHLWEAVAADPTGNML
                     AAQQRAIANFGDARAIAAQFAVVSLARHSRRAGAAAVLVIATVFIAMKARIAWIAAAQ
                     LAIGEDMRAVSGLVVLIDRYAFWLSVIIGLGGWAYIVSRRIPAGFYPAYRKQLRRFFV
                     LCCAAAAALVVSVVSDGVLTALLLRGTELSAGFLVPVFSMAIEIVCVGILVFHIRGIT
                     RRTASTAALLNT"
     gene            complement(1800..2135)
                     /locus_tag="VN968_09335"
     CDS             complement(1800..2135)
                     /locus_tag="VN968_09335"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL29473.1"
                     /translation="MLKGHLDMIVLAALSAAGPAHGYAVIEEIKRRSGGAFDLPEGTI
                     YPALHRLELAGLLKSSWMTADSGRRRRVYTLTRAGNRALIERRAVWKQFSDAIGGLLG
                     GTRPRENPA"
     gene            complement(2252..2611)
                     /locus_tag="VN968_09340"
     CDS             complement(2252..2611)
                     /locus_tag="VN968_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HXL29474.1"
                     /translation="MNTINRSNDDVQSASNGGLNRRTVLMTAAVAASAAAGVSTAPAD
                     TAPAVSFGAPLVELQVPAGVLSLEQKAAMIKGITDVVLRATKQPSDPARKSFVQIFET
                     ADGGFGVNGQVFVPRGK"
     gene            complement(2710..2904)
                     /locus_tag="VN968_09345"
     CDS             complement(2710..2904)
                     /locus_tag="VN968_09345"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HXL29475.1"
                     /translation="MPVAKVYLPEGLLTPDQRREIVRGIHDVIVSVEKRPADSPTYVL
                     INEVPAGDWGATGNVYTPRT"
     gene            complement(3033..>3325)
                     /locus_tag="VN968_09350"
     CDS             complement(3033..>3325)
                     /locus_tag="VN968_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiE"
                     /protein_id="HXL29476.1"
                     /translation="VPFSCHQIDPIKELLIAADFSDIGIAVVGLEKQIPDMALFARGL
                     VYGNPLIDQIRTRGGVEPELIVDALLQALRHEFGVDPGRMPLQAIVFSATKP"
BASE COUNT          647 a         1061 c          999 g          618 t
ORIGIN      
        1 atcctgtggc tccaaatctt gtcgctcaat ggttcgagcc tgatgcttgc gccaaatcta
       61 ggcttggaga ttgttgctat ccacccggat tccgggggaa cctggcgcca tcaacagatg
      121 gccatcattt attgcgctag cggcacaatt caatgcgccc gagccatggc gagcgttagc
      181 cggtccgcgc aacccgccct gcccttgaag gggaatgggc acacgccaga tttcatggaa
      241 caatcgatca agaccccttc cctcatccgc acgagccctc catgcaaacg cttcacgtca
      301 atgggtacga catggcctat ctcgaagtcg gcaatgggcc tgcgctggtg tgcgtacacg
      361 gctcgttgtg cgacttccgc atctggtcgt gcgtgctcgg cccgttatcg aggaggcatc
      421 gggtgatcac cgtcagcctg cggcatttct tccccgaaca ttgggacggc gtcggcgaca
      481 cctattcgat cgcgcagcat acagccgacg tgatcgcctt tatcgagaag ctggacaccg
      541 ggccggtgga tctgatggga cactcgcgcg gcggacacat ctcgtttcgg gtggcgcagc
      601 agcggcctga tctgttgcgc agactgatac tggccgagcc cggcggcgaa ctcgatgcca
      661 cgctcgatcc ggcattcaag cccggcccct ccccgctcgc agcgaggatc gcagcaacag
      721 ctgagaaaat cgccgccggg gatatcgacg gcggactgat gctgtttatg gatgcactcg
      781 aaggccccgg cgcgtggaag cggctgcccg caacgccgaa gcagttgttg cgcgacaatg
      841 ccatgacgct gctgggccag accggggaca agcgcccgcc gttttccttg gccgacgcgg
      901 aggcaatcaa ggcaccgacg ctgttcatcg gcggcgccaa taccaagggc gcgttgcccc
      961 aggtgctgca cacgctggcg gcgcatgttt cgaattcgag gaccgcgatg attcccggcg
     1021 ccacgcatcc gatgttcgaa caggcgccgc aaaagttctg cgagatcgtg ctggagtttc
     1081 tgggagcttg ataaccagcc ctgctcgacg tcacgtgttc agcaaggccg ccgtggacgc
     1141 cgtccgccgg gtgatgccgc gaatgtgaaa gacgagaatt ccgacacata cgatttcgat
     1201 cgccatcgag aacaccggga ccagaaatcc cgctgataat tccgttccgc gcaacagcag
     1261 tgcggtgagc acgccatcgc tgacgaccga aacgacaagc gctgcggccg ctgcacagca
     1321 cagcacaaag aagcggcgga gctgtttgcg atacgccgga tagaacccgg cgggtatccg
     1381 gcggctgacg atgtaggccc agccgccaag gccaatgatg accgacagcc agaatgcata
     1441 gcgatcgatc agaaccacca ggccgctgac cgcccgcatg tcctcaccga tggccaactg
     1501 cgccgctgca atccatgcaa tgcgggcctt catcgcgatg aagacggtgg caatgaccag
     1561 aaccgcggcg gcgccagccc ttcgactgtg cctggccagc gagaccacgg cgaactgggc
     1621 cgcgatggcg cgcgcatcac cgaaattcgc gatggcgcgc tgctgcgccg caagcatatt
     1681 ccctgttgga tcggccgcga cggcttccca caaatggtct tcgacttctt gccgcacgtt
     1741 tcgcgcaagc gatcgatcga aactgagctc gcgcgcgagc cgttcgagat agtcgggaat
     1801 catgccgggt tctcccgtgg ccgggttcct ccaagcagac cgccaatggc atctgaaaat
     1861 tgtttccaga ccgcgcggcg ctcgatcagc gcacgattgc cggctcgggt cagtgtgtag
     1921 acccgccgcc gccgcccgga gtcggcggtc atccagctgc ttttgagcag gcccgccagc
     1981 tcgagacgat gcagcgcggg gtagatcgtc ccctcgggca gatcgaacgc gccgccactt
     2041 cgccgcttga tctcctcgat gacggcgtag ccatgcgccg ggcccgccgc cgacagcgcg
     2101 gcgaggacga tcatgtcgag gtgccccttg agcatttcgc cttccatacc ttgtattact
     2161 aggcgtcggt acccaagcgg tcaagccgag aaccgctgcg cacgctcgcg ccaaacatga
     2221 tcgttttcgt ttcgccagct gaagtcgcag cctacttgcc tcgcggcacg aacacctgac
     2281 cattgacacc gaagccgccg tcggcggtct caaagatctg cacaaacgat ttccgtgccg
     2341 gatccgacgg ttgcttcgtc gccctgagaa cgacgtcggt gatgcctttt atcatggccg
     2401 ccttttgctc aagcgaaagc acgccggcag gaacctggag ctccaccagc ggtgcgccga
     2461 agctgacggc cggagcagtg tccgccggcg ccgtcgagac accggccgcc gcactcgcgg
     2521 cgacagccgc cgtcatcagg acagtcctgc gattcaatcc accgttgctt gctgattgca
     2581 cgtcgtcgtt cgacctgttg atggtgttca tggtccgacc tcgcaatggt gatgggttat
     2641 cgccgccgcc cgcatgcggc ttgccgcgct cttggcggaa tgccggaagc ggaatggagg
     2701 aaattgtcgt caggtccggg gcgtgtagac gttgccggtg gcgccccaat cgccggccgg
     2761 gacctcattg atcagcacgt aggtcggcga atccgctggc cgcttttcga cgctgacgat
     2821 cacgtcgtga atccccctga cgatctcacg ccgctgatcc ggtgtgagaa ggccttcggg
     2881 cagataaact ttggcaaccg gcatggcagg ctccttcctc gcgctctata ccttgcaacg
     2941 atatgcctcg taatactagg tgtcaaggct acactcaggc cggctgccgc atggcgacgt
     3001 tgaggcgatt cagacgttga tggctaagcc tgctagggct ttgttgcaga aaacacaatg
     3061 gcctgcagcg gcatgcggcc gggatcgacg ccgaattcat gacgaagcgc ttgtagcagc
     3121 gcgtcgacga tcaattcggg ctcgacgccg ccccgtgtcc ggatctgatc gatcagcgga
     3181 ttaccgtaga ccagcccccg cgcaaaaagc gccatgtccg gaatctgttt ttccaatccg
     3241 accacggcaa tgccgatgtc gctgaagtca gccgcgatca acaattcctt gatcggatcg
     3301 atctgatggc aggagaacgg cacgt
//
LOCUS       DATNOI010000246        11582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1080163,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000246 DATNOI010000000
VERSION     DATNOI010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11582
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080163"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VN968_09355"
     CDS             <1..316
                     /locus_tag="VN968_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="class II poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HXL29477.1"
                     /translation="PWKGVHKTAQIMGEGTTFVLSNSGHLQSLLNPPTNPKASFMIGA
                     VHPSGPDAFVASAEKRKGSWWLDWRDWLHARSGEEVAAAASPGSERHPVLAAAPGTYV
                     FD"
     gene            282..1265
                     /gene="phaZ"
                     /locus_tag="VN968_09360"
     CDS             282..1265
                     /gene="phaZ"
                     /locus_tag="VN968_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006112 - energy reserve metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxyalkanoate) depolymerase"
                     /protein_id="HXL29478.1"
                     /translation="MLQLRGPTSLTNDPTGGHRVATPEIEARQIAIEGQLLQVAIRRG
                     SGQQPPLLLFNGIGANWEVAKPFLEALTSTTAIIFDVPGIGGSPRPLLPYRPSTLARL
                     AAALVAELAYTEIDVAGVSWGGGIAQQFAHQYPKLCRRLVLAATAPGFTMVPASPAVL
                     WKMATPRRYTDKDYMNRIAADLYGGAFRYDPSLIGRHAAAMHGARNLGYLYQLLAMSG
                     WTSLPWLWSLPQQTLVLMGRDDPLVPPINGQILAGLIPNAELRMIDDGHLFIVTRPVE
                     TAALIEAFLADASKHVDPSSPLSRLASWVRDLISTFGGRRDNQAQQGGRKS"
     gene            1262..1690
                     /gene="phaP"
                     /locus_tag="VN968_09365"
     CDS             1262..1690
                     /gene="phaP"
                     /locus_tag="VN968_09365"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HXL29479.1"
                     /translation="MTDTISYIEMLRKLGAALGLPKPNVDQLVETQTKNIEAFGQSAQ
                     LVAQGAQSVAQKQRERMKAGLREAMAFARENQALGRVNANLAQQTEVARKIFEIAVKG
                     AQETAATARQSGTDAVKIVQDRMKEALEGMRASIGRNEPT"
     gene            1783..3002
                     /locus_tag="VN968_09370"
                     /pseudo
     CDS             1783..3002
                     /locus_tag="VN968_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            3460..3735
                     /locus_tag="VN968_09375"
     CDS             3460..3735
                     /locus_tag="VN968_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29480.1"
                     /translation="MQFRHLLLSLAVALIVAGPLPARADAPAAKDVGTIGDCLRKQDK
                     KRGSQEAEEAACLMTVAKPCMGGDETGVSSGRHPDRERLVWDKIING"
     gene            3735..3962
                     /locus_tag="VN968_09380"
     CDS             3735..3962
                     /locus_tag="VN968_09380"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HXL29481.1"
                     /translation="MNALAPDQQDKLREMQLSWIHTRDLACAFWYDYFQGTMVNPMIA
                     YCNNRETARRAIFLRIFAADIAERSGNSRPE"
     gene            3974..5047
                     /locus_tag="VN968_09385"
     CDS             3974..5047
                     /locus_tag="VN968_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXL29482.1"
                     /translation="MSSTIQPATMKGPAIFLAQFIGGAPPFDSFDAICGWAAGLGYKG
                     VQVPTSDPRLIDLDKAAASQTYCDEIAGVAASHGLVITELSTHLQGQLVAVHPAYDEA
                     FDAFAPSAVRGRPKERQIWAVHQLVKAAQASRRLGLRGSVSFTGALAWPFLYPWPRRP
                     PGLIEIAFDELARRWRPILDAYDEAGVDLCYEIHPGEDVCDGITFEMFYQRTGNHPRC
                     AINYDPSHFVLQQLDYLAFIDIYAERIRAFHVKDAEFNPTGRQGVYSGFQPWIGRAGR
                     FRSLGDGQVDFKAIFSRMAAIGFDRWAVLEWECCLKNAEDGAREGAGFIRDHIIRVTD
                     RAFDDFAGGVVDGAQTRRMLGLV"
     gene            5061..6212
                     /locus_tag="VN968_09390"
     CDS             5061..6212
                     /locus_tag="VN968_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXL29483.1"
                     /translation="MAEPRIRLGMVGGGEGAFIGAVHRIAARLDDRYELVAGALSASP
                     DKAMRSAAAIGLRPDRSYPDFRTMARAEAARPDGIEAVAIVTPNHLHALVAEAFLEAG
                     IHIICDKPLTATLAEARLLRDKARAQGLIFAVTYNYSGYPLVRHARAMVQAGEVGAIR
                     IVQVEYPQDWLAGPLEATGQKQADWRTDPVRSGPGGCIGDIGTHAYQLAYFVSGMMPE
                     QILAEVTTFVPGRRVDDNVQILLRYPNGARGALWASQVAPGQDNTLRLRVFGENGALD
                     WRQEEPNCLIWSPLGEAPRLIRRGTAAMNSSGQRVSRIPPGHPEGYLEAFATIYTEVA
                     ACIMARRTGSSIDPAVTFPTIEDGFAGVAMVDAALRSSAAGGLWMATAR"
     gene            6420..7694
                     /locus_tag="VN968_09395"
     CDS             6420..7694
                     /locus_tag="VN968_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXL29484.1"
                     /translation="MGLLTAGLLQRLVLAAAVLTVAAAGAAAETTVKWLHIEANPAQV
                     KIWEEVARTYEAGHPGVKIEMQFLENEAYKAKLPTILQSKDRPHIIYSWAGGVLKSQI
                     EAGVLEDITDQVKGFGDTITPAALAVFSQNGRVYGLPIALSQVGFLYNKDLMAKAKVD
                     AATIRTWGDLLAAVKALKAAGVTPIVVGGADKWPLHFYWTHLAVRIGGKAAFDAALRG
                     ENGGFAGATFQQSGELFKQLVDLQPFQNGFLGFKNPQAVGYFGDGKAAMTLAISTTYH
                     LQRALAADKVGLGEDKIGWFDFPVVPAGKGAPSDTLGGITGWLITKSAPQEAVDFLRS
                     FISRDVQTRLAAGNFLIPVVKGADAGLNNSFMKRIAENLAKSNYHQNFYDQSLGPSVG
                     RVVNDAAAEIAGGSMTPQQAAAAIEAAWKQGN"
     gene            7736..8638
                     /locus_tag="VN968_09400"
     CDS             7736..8638
                     /locus_tag="VN968_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018114809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL29485.1"
                     /translation="MRSSMGPLPVRRSLRDGRFAVLVLFLPPALVLFTLFVVLPIGEA
                     AWYSGFNWNGFGKPTNWIGLDNYRFVLETRAFWLALRNNGLIIAVSLLIQLPLALALA
                     LTLAERFRGAIALRMLFFLPYILAEIATGLIFSFVYDGDYGLVASIWHAFGAEAPYLL
                     ASTDTAMLAVLVVIVWKYFGFHMMLFIAALQGMDRNLVEAARIDGATRPQLLGYVVIP
                     LLYPTIRLSVFFAIVGSLQLFDLVMPLTRGGPADSSNTMVSFLYNNGISRMRVGYGSA
                     IGVILFVICVTFAFTYKRWFMRDE"
     gene            8631..9479
                     /locus_tag="VN968_09405"
     CDS             8631..9479
                     /locus_tag="VN968_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL29486.1"
                     /translation="MSNVETTRPAVDLAVLHKALFLALVATFVLVPLLATVLGGFKSL
                     GELRVNPFGLPHHWEWSNYADILVSKRYWQLLRNSMIISTLTVTLTLIVASMAAFTFA
                     HVKFFGSSMLLSYLTLGLLFPAATAVLPLFIKVRDLGLLDSYFGVALPQVAFSLAMSI
                     LLLRRFFKDLPHELLEAARVDGCSYINFFRYVTLPLSRPILATVGTITFVNSWNAYLL
                     PLVMLNTDELYPWPLGIMVYQGEYSSEWHLILAFITLTILPTIILFLFAQKHIVAGLT
                     AGAVKG"
     gene            9520..10617
                     /locus_tag="VN968_09410"
     CDS             9520..10617
                     /locus_tag="VN968_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXL29487.1"
                     /translation="MRKVGIGLIGCGNISAAYLKAAQRFPILDIKAVADMRSEAAEKR
                     GAEFGLPWMRVDQLLKRDDIEIVINLTVPLAHTDVSLAVLNAGKHVHSEKPLGVNVAE
                     ARKVMDLAAQKGLRVGCAPDTFLGGGHQTARKLIDDGAIGTPVAGSAFFMCPGHERWH
                     PAPGFYYLRGGGPMLDMGPYYITDLVQLLGPVASVMGSTARPKSERLVTSEPMKGKLI
                     PVEVATHVAGTLEFESGALVSVAMSFDVLNHRHAPIEIYGDSGSMLVPEPNRFGGEVQ
                     VAKTGGEWESVRLTHGHVDGEFRSIGVADMATAIVTDRPHRASGALAFHVLEVMEAFQ
                     TSSDEGRRVKIGSRVDRPAMMPAGRETGQID"
     gene            10630..11271
                     /locus_tag="VN968_09415"
     CDS             10630..11271
                     /locus_tag="VN968_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HXL29488.1"
                     /translation="MRKAMIVCGGWPGHDPDLCASMIRGWLKREGFDVRIETTTAAFA
                     DPAIHDLSLIIPIYTMSKIEKPEALNLCAAVQSGVGLAGHHGGMCDAFRDSVDYQFLC
                     GGQWVAHPGNIIDYRVDLTKPDDPIMQDLKSFEHRSEQYYMHVDPANEVLATTTFTGE
                     HAPWIEDVVMPVVWKKRYGEGRVFYSSLGHRAYELDVPEIRTIMTRGMLWAAR"
     gene            11271..>11582
                     /locus_tag="VN968_09420"
     CDS             11271..>11582
                     /locus_tag="VN968_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXL29489.1"
                     /translation="MARGETSPAGRARLEDVARAAGVSPATVDRVVNRREGVRQKTIA
                     RVEAAVAKLGYRADPAATRLARNQSFRFAFILPTGSNSFMVNLTEQVKRTADWLAGQR
                     AF"
BASE COUNT         2100 a         3602 c         3747 g         2133 t
ORIGIN      
        1 tccttggaag ggtgtgcaca agaccgccca gatcatgggc gaggggacga ccttcgtgct
       61 ttcgaacagc ggccacctcc agagcctcct caacccgccg accaatccga aggcctcctt
      121 catgatcggg gcggtccatc cgtctggtcc agacgcattc gtggcctcgg ccgagaagcg
      181 taagggaagc tggtggctcg actggcgcga ttggctgcac gcacgatcgg gcgaagaggt
      241 tgcggcagcc gcgtcgccag gtagcgaacg ccatcccgtc cttgctgcag ctccggggac
      301 ctacgtcttt gactaacgac cctacaggag ggcaccgagt cgcgacgccc gagatcgagg
      361 cgcggcaaat cgcgatcgag gggcagttgc tccaggttgc catcagacgc ggcagcggcc
      421 aacagccgcc gctgttgctc ttcaacggaa tcggggccaa ctgggaggtg gcgaaaccct
      481 tcctggaagc actcacgagc acgacggcaa tcatctttga cgtgcctggc atcggcggtt
      541 cgcccaggcc gctgctgccg tatcgcccat cgacgctggc tcggcttgcc gccgctctcg
      601 ttgcggagct cgcctacaca gagatcgacg ttgccggcgt gtcctgggga ggtggaatcg
      661 cgcagcagtt cgcgcatcaa tatccaaagc tgtgccggcg gctggtgctc gccgcgaccg
      721 cacccggttt cacgatggtg ccggcaagtc ccgcggtgct gtggaaaatg gcgacgccgc
      781 gccgctacac cgacaaggat tatatgaaca ggatagcggc ggacctctac ggaggagcct
      841 ttcgctacga cccatccttg atcggccggc atgcggccgc gatgcatggc gcgcgcaacc
      901 tgggctactt gtatcagctt cttgcgatga gcggctggac cagcctgccg tggctgtggt
      961 cgctgccgca gcagacgctg gtgctgatgg gccgcgacga tccattagtc cctccgatca
     1021 atggtcagat tctggcgggc ctgatcccga acgccgagct ccggatgatc gatgacgggc
     1081 acctgttcat cgtgacgcgg ccggtggaaa ccgccgccct gatcgaggca tttctggccg
     1141 acgcgagcaa gcacgttgat ccatcatctc cgctttcacg attggctagt tgggtcagag
     1201 atctcatttc gacattcgga ggacgccgcg ataaccaagc gcagcaagga gggaggaaat
     1261 catgacggac acgatctcgt acatcgagat gctgaggaaa ctcggggcgg ctcttggctt
     1321 gccgaagccg aacgtcgacc aactggtgga gacccagacg aagaacatcg aagcgtttgg
     1381 ccaaagcgcc cagctagtcg ctcagggcgc gcagtcggtc gcgcagaagc agcgcgagcg
     1441 gatgaaagcc gggttgcgag aggctatggc gttcgcccgc gagaaccagg cgctcggaag
     1501 ggtcaacgcg aatctagcgc agcagacgga agttgcgcgc aagatctttg aaattgcagt
     1561 caagggggct caggaaaccg cggccaccgc gcggcaatcc ggtaccgatg ccgtgaagat
     1621 cgttcaagac cgcatgaaag aggctcttga ggggatgcgc gccagcattg gtcgaaacga
     1681 gccgacctga accaggagga tttccggcaa agcgacacgc cagcttttgc tcggagccgc
     1741 caaacgcagg caggcttcac cactcagcgc agggaggaac cgatggcgac tcagcaggca
     1801 aaatcaatcg ggaaaacgga ctacgctccg cctccaatcg aaggtgattt ctacaggata
     1861 gccgccgtac tgaacgacag cgagcggaca ctgctcaaac gtgttcgcga gttcacggaa
     1921 ggcgtcatcg cgccattgat cgaggattat tggagccgcg atgcatttcc cttcgagatc
     1981 attccgaaga tggcgcaagt cggtatcgga ggggtgggct atcagggaaa cggcgccgcc
     2041 ggcggcggcc gctggctgct gaacggcctt gtcgcgatgg agttggcgcg tgtcgatgcg
     2101 tcggtcgcga cgttctgggg cgtgcacacg ggcctttcgg ccggatcaat ctatctgtgc
     2161 ggggacgagc agcagaagca acgctggctg ccgcctatga tgcgcttcga caagatcggc
     2221 tcgtttggct tgacggagcc gctagtgggg tcggcgacat caggtggaat gatgacgaca
     2281 tgccgccggg aaggcgacag ctggatcctc aacggccaga agaagtggat cggaaattcc
     2341 acgttcgccg acatcaacgt gatctgggca cgagaggaag actcgaacca ggtcaagggc
     2401 ttcgttgtgg gaaaggacaa tccgggcttc tcggtcgaga agatcaagac caagatggcc
     2461 ttgcgggtcg tgcagaacgg gttgatcacc ctgagcgact gctgcgttcc cgaggccgac
     2521 cgcttgcaga acgccaactc gttcaaggac accgccaagg ttctcaggat gacgcgcacc
     2581 agcgtggcgt ggttcgccgt aggctgccag atgggagcat acgagcacgc gctccgctat
     2641 gcgacggagc ggacgcaatt cgggaggccc atcggcggat tccagctcgt ccaggatctg
     2701 ctggtgcgca tgcttggcaa cgtcacatcg acccaggcga tgatgctgcg tcttgcgaac
     2761 ttcaagacga aggtgtgatg cgcgatgagc atgcgtcgct cgcgaaggcg ttctgcacgg
     2821 tcaaatgccg cgaaaccgtc ggttatgcgc gcgagttgct cggcggcaac ggcatcctgc
     2881 tcgagaacca catcggacgc ttcgtggccg atgccgaagc aatctactcc tatgagggca
     2941 caagggagat gaacacgctg atcgtcagca agtccattac aggattgagt gccttcgtgt
     3001 gacgccaagc attggcccga tccaccgacc gaaccgccct tttctttgga cgtccccgcg
     3061 catcgaggtc caccggcgat gtctcgctct gtcgatccgg cggatcgcaa tcgcgccgtt
     3121 acgccaattc cctctagcga ttgctctgat aagtcttcag gacgatcggt tcccccgatc
     3181 ggttctttcc ttggccgcca acaagatttc ttttgcggct ttgatgacag ggggttcaag
     3241 ggaagtgatg agaaaatgcg ccatgatctc accccatgcc attcctgact ggccgcttct
     3301 gtgaaggctt tcggacgccg gctgcccgtg catgcggcac cgctcgtgag cggccggccc
     3361 ttcacataaa tggactcacc gcagcagtac ggccgagttc gaaccggcgg tcgggtagcg
     3421 gggaaatctg atttgtcttc gtcggtgggt tcaaggcaca tgcaattccg tcatttgttg
     3481 ttgtcgctcg ccgtcgcgct gatcgttgcc ggcccgcttc cggcgcgggc ggacgctccg
     3541 gcggcaaagg atgtcggcac gatcggcgac tgcctgcgca agcaggacaa gaagagggga
     3601 tcgcaggaag ccgaagaggc ggcctgcctc atgaccgtgg cgaagccttg catgggcggc
     3661 gatgaaaccg gcgtgagctc ggggcgccac ccggatcgcg agcggctggt ctgggacaag
     3721 atcatcaacg gttgatgaac gcgctcgcgc ccgaccagca ggacaagctg cgcgagatgc
     3781 agctatcatg gattcatacc cgcgatctgg cctgcgcgtt ctggtacgat tatttccagg
     3841 gcacgatggt caatccgatg atcgcgtatt gcaacaaccg cgagaccgcc cggcgcgcga
     3901 tcttcctgcg gatttttgcc gcggatatcg cggaacggtc cggcaattcg cggcctgaat
     3961 aggagagagt gcgatgtcat cgacgattca accggctacc atgaaggggc cggcaatctt
     4021 cctggcgcag tttatcggcg gtgcaccgcc gtttgacagc ttcgacgcca tctgcggctg
     4081 ggctgcaggg ctcggttaca agggcgtcca ggtgccaacc tccgatccgc gcctgatcga
     4141 cctcgacaag gcggcggcct ctcaaaccta ctgcgatgaa atcgccggcg tggccgccag
     4201 ccacggtctg gtgatcaccg aattgtccac ccatctgcag ggacaactgg tcgccgttca
     4261 tccggcctat gacgaggctt ttgacgcttt cgcgccatcg gcggttagag gcagaccgaa
     4321 ggagcgtcag atctgggcgg tgcaccaact cgtgaaagcc gcgcaggcct cgcgccggtt
     4381 aggcctccgc ggcagcgtca gcttcaccgg cgcgctggcc tggccgttcc tctatccatg
     4441 gccgcggcgg ccgccgggat tgatcgagat cgccttcgac gaactggctc gccgctggcg
     4501 tccgatcctg gacgcctatg acgaggccgg tgtcgatctc tgctatgaga ttcatccggg
     4561 cgaagacgtc tgcgacggca tcaccttcga aatgttctac cagcgcaccg gcaatcatcc
     4621 gcgttgcgcg atcaactacg atccctcgca tttcgtcctg cagcaactgg actacctcgc
     4681 cttcatcgac atctatgcgg agcgcatccg cgccttccac gtcaaggatg cggaattcaa
     4741 tccgaccggc cggcagggtg tctattcggg tttccagccc tggatcggtc gggccggccg
     4801 tttccgttcg ctcggcgacg ggcaggtcga cttcaaggcg atcttctccc gcatggctgc
     4861 catcgggttt gaccgctggg cggtgctcga gtgggaatgc tgcttgaaga acgccgagga
     4921 cggtgcccgc gagggcgcag gctttatccg agaccacatc atccgcgtca ccgaccgggc
     4981 gttcgatgat ttcgccggcg gcgtggttga cggcgcacag acacggcgca tgctgggtct
     5041 ggtctgagca ggaggacacg atggctgagc cgcgcattcg actggggatg gtgggcggcg
     5101 gggagggcgc cttcattggc gccgtccacc gcatcgccgc gcgccttgat gaccgttacg
     5161 aactggtggc cggggcgctc tccgcctcgc ccgacaaggc gatgcgctcg gcggcggcaa
     5221 tcgggcttcg gccggatcga tcttatccgg acttccggac catggcgcgg gccgaggcgg
     5281 caaggcccga cggcatcgag gcggtggcca tcgtcacccc gaaccacctg catgccctcg
     5341 tcgccgaggc atttctggag gcgggtattc atatcatctg cgacaagccg ctgacggcga
     5401 ccctggcgga ggcgcgcctt ctgcgcgaca aggcgcgcgc gcaagggctg atcttcgctg
     5461 tcacctataa ttattccggc tatcccctgg tccgtcacgc ccgcgccatg gtgcaggccg
     5521 gtgaagtcgg cgcgatccgg atcgtgcagg tcgaataccc gcaggattgg ctggccggcc
     5581 cgctcgaggc caccggccag aagcaggccg actggcgtac cgatccggtc cgctccggtc
     5641 ccggcggctg catcggcgac atcggcacac acgcctacca gctggcgtat tttgtcagcg
     5701 gaatgatgcc cgaacaaatc ctcgctgagg taaccacctt cgtgccgggc cgacgcgtcg
     5761 atgacaatgt gcagattttg ctgcgctacc ccaatggagc gcggggcgcg ttatgggcga
     5821 gccaggttgc gccgggtcag gacaatacac ttcgcctgcg ggtcttcggc gaaaacggtg
     5881 cgctggactg gcgccaggaa gagccgaact gcctgatctg gtcgccgctc ggcgaggcgc
     5941 cgcgattgat ccgtcgcggc acggcggcga tgaactccag cggtcagcgc gttagccgca
     6001 tcccacccgg ccatcccgag gggtatctgg aggcgtttgc aacgatctat accgaggtcg
     6061 ctgcgtgcat catggcgcgc cggaccggtt cctccatcga tccggcggtg acctttccga
     6121 caatcgagga cggttttgcc ggtgtcgcga tggtggacgc cgccttgcgc tcaagcgcag
     6181 ccggcggtct ctggatggcg acggcgcggt gaatgccgag ctgacgtcgt gaggcgcttg
     6241 ttgatcgcgg cgcattcaca cccagccgcc atccacgaca taatgctgcg cggtgcaggc
     6301 cgagggaagc ttaatcggtc ggcggttgat ggttatagat caaaatctga gttgcgcacc
     6361 tcaaaaaatg cggtagcttc gcgccaaccg gccacctgac gccggatcgg aggattctca
     6421 tgggactgct gacagcagga ttgctgcaaa ggctggtgtt ggctgccgcc gtgctgacgg
     6481 tggcggccgc gggcgccgcg gcagagacca ccgtgaagtg gctccatatc gaggccaatc
     6541 cggcccaggt gaagatctgg gaggaggtcg cgcgcaccta tgaagccggc catcccggcg
     6601 tcaagatcga gatgcagttc ctcgagaacg aggcgtacaa ggcaaagctg ccgaccatcc
     6661 tgcaatccaa ggaccggccg cacattatct atagctgggc gggcggcgtc ctgaagtcgc
     6721 agatcgaggc tggcgtcctc gaagacatca ccgatcaggt gaagggtttt ggcgacacca
     6781 tcacgccggc ggcgctcgcc gtcttcagcc agaacggccg tgtctacggc ttgcccatcg
     6841 cgttgtcgca ggtcggcttc ctctacaaca aggacttgat ggcgaaagcc aaggtcgacg
     6901 ccgccacaat caggacctgg ggcgatctgc tggccgcggt gaaggcgctg aaggcggccg
     6961 gcgtcacgcc gatcgtggtc ggcggcgccg acaagtggcc gctgcatttc tactggaccc
     7021 accttgcggt gcggatcggc ggcaaggcgg cgttcgatgc ggcgctgcgc ggcgagaacg
     7081 gcggcttcgc cggagcgacg ttccagcagt ccggcgagct gttcaagcag ctcgtcgacc
     7141 tgcagccgtt ccagaacggc ttcctcggct tcaagaatcc gcaggccgtc ggatatttcg
     7201 gcgacggcaa ggccgcgatg acgctggcca tcagcacgac ctatcatctg cagcgcgcgc
     7261 tcgccgccga caaggtcggc ctcggcgagg acaagatcgg ctggttcgat tttcccgtcg
     7321 tgcccgccgg caagggcgca ccgagtgata cgctcggcgg catcaccggc tggctcatca
     7381 ccaaaagcgc gccgcaggag gccgtcgatt ttctcaggtc ctttatctcc agggacgtgc
     7441 agacacggct cgccgccgga aatttcctga ttcccgtggt gaagggggcc gacgcaggcc
     7501 tcaacaactc cttcatgaag cggatcgccg aaaacctggc gaagtcgaac taccatcaga
     7561 acttctacga ccagagcctc ggtccgtcgg tcggccgcgt cgtcaacgac gccgccgcag
     7621 aaatcgccgg cggcagcatg acgccgcagc aggcggcggc tgcgatcgag gccgcgtgga
     7681 aacagggtaa ctgacggtgc gcggacaaac ggcaggcgcg ccgccgctcg tctccatgag
     7741 atcgtcgatg gggccgctgc cggtccgccg ctcccttcgc gacggccggt ttgcggtgct
     7801 ggtgctgttt ctgccgcccg cgctcgttct gttcacattg ttcgtcgtgc tgccgatcgg
     7861 cgaggccgcc tggtattccg gcttcaactg gaacggcttc ggcaagccga ccaactggat
     7921 cggcctcgac aattaccgct tcgttctgga aacccgcgcc ttctggctcg cgctgcgcaa
     7981 caacggcctg atcatcgccg tctcgctcct gatccagctg ccgctggcgt tggcgctcgc
     8041 gctgacgctg gccgagcgct ttcgcggcgc gatcgcattg cgcatgctgt tcttcctgcc
     8101 ctatatcctg gccgagatcg cgaccggcct gatcttcagc ttcgtctatg acggcgacta
     8161 tggcctcgtc gcatcgatct ggcacgcgtt cggcgccgag gcgccgtatc tgctcgccag
     8221 caccgacacc gccatgctgg cggtcctggt cgtcatcgtc tggaagtact tcggcttcca
     8281 catgatgctg ttcatcgcgg cgctgcaggg catggacagg aatctcgtcg aggcggcgcg
     8341 catcgacggc gccacgaggc cgcagctgct gggctatgtg gtcattccgc tgctctaccc
     8401 gacgatccgg ctctcggtgt tcttcgccat cgtcggctcg ctgcaattat tcgacctggt
     8461 gatgccgctg acgcgcggcg gcccggcgga ttcgtcgaac accatggtga gcttcctcta
     8521 caacaacggc atctcgcgga tgcgggtcgg ctacggcagc gccatcggcg tgatcctgtt
     8581 cgtgatctgc gtcaccttcg ccttcaccta caaacgctgg ttcatgcgcg atgagtaacg
     8641 ttgagacgac ccgccctgcc gtcgacctcg cggtgctcca caaggcgctg tttctcgcgc
     8701 tcgtcgccac cttcgtgctg gtgccgctgc tggcgaccgt gctcggcggc ttcaagtcgc
     8761 tcggcgaact gcgcgtcaat ccgttcggcc tgccgcatca ctgggaatgg tcgaactacg
     8821 ccgacatcct ggtctcgaag cgctactggc agctgttgcg caactcgatg atcatctcga
     8881 cgctgaccgt gacgctgacg ctgatcgtcg cgtcgatggc agcgttcacc tttgcccatg
     8941 tgaaattctt cggcagctcg atgctgctga gctacctgac gctgggcctg ttgtttccag
     9001 ccgcgaccgc ggtgctgccg ctgttcatca aggtgcgcga cctcgggctg ctcgatagct
     9061 atttcggggt ggcgctgccg caggtggcgt tcagtctcgc gatgagcatc ctgttgctgc
     9121 ggcgcttctt caaggatctg ccgcacgaat tgctggaagc cgcgcgggtc gatggctgca
     9181 gctacatcaa cttcttccgc tacgtgacgc tgccgctgtc gcggccgatt ctggccaccg
     9241 tcggcaccat caccttcgtc aacagctgga acgcctatct gctgccgctg gtgatgctga
     9301 acaccgacga gctctatcca tggccgctag gcatcatggt ctatcagggc gaatattcgt
     9361 ccgagtggca tctgatcctg gctttcatca cactgacgat cctgccgacc atcattctgt
     9421 tcctgttcgc gcaaaagcac attgtcgccg gcctcacagc gggcgcggtc aagggatgac
     9481 aggtcgcgcg gcgcgcatcg actaattatg gagcacgcaa tgcgaaaagt cggtatagga
     9541 ctcatcggct gcggcaatat cagcgccgcc tatctgaagg cggcgcagcg ctttcccatc
     9601 ctcgacatca aggcggtggc cgacatgcgc agcgaggcgg cggagaagcg aggggccgaa
     9661 ttcggcctgc cgtggatgcg cgtcgaccag ttgctcaagc gcgacgacat tgaaatcgtc
     9721 atcaatctta ccgtgccgct cgcccacacc gacgtcagcc tcgccgtgct caacgccggc
     9781 aagcatgtgc attcggaaaa gccgctcggc gtgaatgtcg ccgaggcgag gaaggtgatg
     9841 gatctcgcgg cgcaaaaggg tctccgcgtc ggctgcgcgc cggatacatt cctcggcggc
     9901 ggccatcaga ccgcgcgcaa gctgatcgac gacggcgcga tcggcacgcc ggtcgccggc
     9961 agcgccttct tcatgtgccc ggggcacgaa cgatggcacc ctgcgccggg cttctactat
    10021 ttgcgcggcg gcggaccgat gctcgacatg ggtccgtatt acatcaccga cctggtgcag
    10081 cttctgggtc cggtcgcaag cgtgatgggc tcgacggcgc gaccgaaatc ggagcggctg
    10141 gtgacgagcg agccgatgaa gggcaagctt attccagtgg aggtcgcgac ccatgtcgcc
    10201 ggcaccctgg aattcgagag cggcgcgctg gtctcggtcg cgatgagctt cgacgtgctg
    10261 aaccaccggc atgccccgat cgaaatctac ggcgacagcg gcagcatgct ggtgcccgaa
    10321 cccaaccgtt tcggcggcga ggtgcaggtg gcgaagaccg gcggggagtg ggaatcggtc
    10381 cggctcaccc atggtcatgt cgacggcgaa ttccgctcga tcggtgtcgc cgacatggcg
    10441 acggcgatcg tcaccgaccg gccgcatcgc gccagcggcg cgctcgcgtt ccatgttctc
    10501 gaagtcatgg aggcgttcca gacctcctcc gacgaaggtc ggcgcgtgaa gatcggaagc
    10561 cgcgtcgaca ggccggcgat gatgccagcg gggcgcgaaa ccggacagat tgattgaggg
    10621 gcaacaacaa tgcgcaaggc gatgatcgtg tgtggcggct ggccgggaca cgatcccgat
    10681 ctgtgcgcct cgatgatacg gggctggctg aagcgggagg gattcgacgt ccggatcgag
    10741 accacgacgg cggcatttgc cgatccggcg atccatgacc tgtccttgat cattcccatc
    10801 tacaccatgt cgaagatcga gaagccggaa gccctcaatc tgtgtgcggc ggtgcagagc
    10861 ggcgtcgggc tggccggcca tcacggcggc atgtgcgatg ccttccgcga ttcggtggac
    10921 tatcagtttc tctgcggcgg ccagtgggtg gcgcatccgg gcaacatcat cgactacagg
    10981 gtcgacctga cgaagccgga tgatccgatc atgcaggatc tgaaaagctt cgagcaccga
    11041 tccgagcagt attacatgca tgtcgacccc gccaacgagg tgttggcgac cacgaccttc
    11101 accggcgagc acgcgccctg gatcgaggac gtggttatgc cggtggtgtg gaagaagcgc
    11161 tatggcgagg gccgggtgtt ctactcctcg ctcggccatc gcgcctatga actcgacgta
    11221 ccggagatca ggacgatcat gacccgcggt atgctgtggg cggcacgctg atggcgaggg
    11281 gtgaaaccag tccggccgga cgcgcgaggc tcgaagacgt cgcgcgcgcg gccggcgttt
    11341 cgccggcgac tgtcgaccgc gtggtcaacc gacgcgaggg ggtgcgccaa aaaaccatcg
    11401 cgcgcgtgga agctgccgtt gccaagctcg gctaccgcgc cgatcccgcg gcgacgcggc
    11461 ttgcgcgcaa ccagagcttc cgcttcgcgt tcatcctgcc gaccggcagc aacagtttca
    11521 tggtgaacct gaccgaacag gtcaagcgca cggcggattg gctggccggc cagcgcgcct
    11581 tc
//
LOCUS       DATNOI010000247         8276 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1749957,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000247 DATNOI010000000
VERSION     DATNOI010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8276
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749957"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="VN968_09425"
     CDS             complement(<1..145)
                     /locus_tag="VN968_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXL29490.1"
                     /translation="MPQTIHRGIKALIDEANAEIETLSATEAIKAAQSDDVVIVDIRD
                     PREI"
     gene            complement(289..1464)
                     /locus_tag="VN968_09430"
     CDS             complement(289..1464)
                     /locus_tag="VN968_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXL29491.1"
                     /translation="MFLQFFTSLRDAQVPVTLREYLTLMEALNADLAEQTVENFYYLA
                     RTALVKDERNLDKFDRVFGTVFKGLESLLDAMEKADIPAEWLKKLAEKYLTEEEKKQI
                     EAMGWDKLMETLRQRLKEQKGRHQGGNKWIGTAGTSPFGAEGYNPEGIRIGQEKNRNH
                     RAVKVWDRREFKDLDGNVELGIRNIKIALRRLRKFARTGAPDELDLDTTIKETANHGY
                     LDVHMRPERRNAVKVLVFFDIGGSMDSHIEQVEELFSAAKTEFKHMEYFYFHNCLYEG
                     VWKQNRRRFTDRTPTWDVLHKYPHDYKVVFVGDASMSPYEIMVPGGSVEHVNEEAGSV
                     WLERITRTYPHAVWLNPVAQRHWDYSESTTLIRRLFSERMFPITIEGLEGAMKELVR"
     gene            1649..2491
                     /locus_tag="VN968_09435"
     CDS             1649..2491
                     /locus_tag="VN968_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXL29492.1"
                     /translation="MKFTGTKDYVATDDLKIAVNASIVLERPLLIKGEPGTGKTVLAE
                     EVAKALGAPLLTWHIKSTTKAQQGLYEYDAVSRLRDSQLGDARVSDISNYIRRGKLWE
                     AFTHEKRPVLLIDEIDKADIEFPNDLLLELDRMEFFVYETGENIKAKLRPIVMITSNN
                     EKELPDAFLRRCFFHYIKFPDIDTMNRIVDVHFPGIKKRLVEEALRIFFEVREVPGLK
                     KKPSTSELLDWLKLLLNEDMTPEMLRERDPRKLIPPLHGALLKNEQDVHLFERLAFLS
                     RREV"
     gene            2561..4063
                     /locus_tag="VN968_09440"
     CDS             2561..4063
                     /locus_tag="VN968_09440"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL29493.1"
                     /translation="MNVQQQIRAASADSTEHFGVVIAGAGISGVGAAYHLTKQCPGTS
                     FVVLETQASFGGTWRTHRYPGIRSDSDLHTFGYRFKPWTSAPIATAAEILSYMGEVIE
                     ENGLGRHIRYRHRISSARWSSEENLWTIEAVRTDSGEAVRFTANFFWMCQGYYRHAEG
                     YTPEWKAMETFKGRIVHPQQWPEDLDYKDKRVVVIGSGATAATLIPAIANDSAHVTML
                     QRSPTYFRTGRNAIDIAETLRTLQVDETWIHEITRRKILYDQAEFTRKTFEEPEAAKK
                     DLLSAVEAYLGPDYDIATHFTPKYRPWRQRIAFIPDGDLFQGIKSGKASVVTDEIDRF
                     TETGILLKSGKTLEADIIVTATGFNLSANGDMEFGVDGKPLDFADTVTYRGMMFTGVP
                     NLVWVFGYFRASWTLRVDLVADFVCRLLDHMKQTGARKVTPAMRAEDHNMALLPWIDP
                     ENFNPGYMMRGMHLLPKRGDKPEWQHNQDYWAEKDEFPAIDLNDRAFVYE"
     gene            complement(4084..5313)
                     /locus_tag="VN968_09445"
     CDS             complement(4084..5313)
                     /locus_tag="VN968_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL29494.1"
                     /translation="MQACPANPPSEARATVLPALSQPRSLPPRAMTLFSFGGAMLLAA
                     SSSAATPLYRLYQQSMHLTPFWITIVFAVYVASLLAGLLTVGGLSDYVGRRPVILAAL
                     LLNAAAMLLFAEARDVGQLILARAVQGLCVGTGTTALGAAILDTNRARGPLLNSVTAF
                     LGMTAGSLGAAALITFAPDPLHRVYEVLLGLTALMIALLWVMPESTPRKAGALASLRP
                     QIGVPRQSRSVLIRLTPANVAAWALGGLYLSLMPTVVATAMGVVSPWIGGVVVSALML
                     TAAIAVAAFREWPARRLILTGTATLSLGVAVSMFGIQQQQVAALLAGTVIAGAGFGST
                     FSGTLRALLPTAEPDQRAGLLSAFYVQSYLAFSLPAVAAGLSVPLIGLSAAAYLYGTV
                     IILLAITSMMASLRTDR"
     gene            5435..6001
                     /locus_tag="VN968_09450"
     CDS             5435..6001
                     /locus_tag="VN968_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL29495.1"
                     /translation="MAVKERVRVGGRSARIQSAVHEAVRNLSLKTNRDELTVPQIAAE
                     AGVTPSTIYRRWGDLSALLADVAVQRLRPIADPEDTGAVASDLRVFIEQFMEEMSSPV
                     GRALIRDVFSPPGETYPVQCAGFTYEHLKTIVSRAKARGEPPFDIDEVIDHVVAPIIY
                     HILYGNRELTLDYCHCLLDRLQSLNPKY"
     gene            complement(6019..7665)
                     /locus_tag="VN968_09455"
     CDS             complement(6019..7665)
                     /locus_tag="VN968_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXL29496.1"
                     /translation="MDTFDYVIVGAGSAGCVLTNRLSEDQGNSICVLEAGPRDWHPYI
                     HLPAGFIKTFHMKRINWAYQQEPGPWTGGRSIYAPRGKTLGGSSSINGHIYNRGQRQD
                     FDTWAQLGNRGWGYPDVLPYFKRLERRVGEGEDAFRGRDGNLTVTTMEWRDPLCEAFM
                     AGAMSLGIPRNPDYNGAIQEGVSYAQRTIKNGLRVSAATAFLHPAMKRPNVHVRTHAH
                     ATEIVFDGKRAIGVRYAKGGKGGAVTEVRAAREVILAGGTYNSPQLLQLSGVGSPELL
                     ASLGIAVRHALPGVGEGLQDHYAPRSVARVKNIKTINERRRGLSLAVEAIKWATTRRG
                     LLSLSPTMVYCFWHSGETTESSDLQLTFTPASYKEGVQGQLENEPGMTVASWQQRPES
                     RGYVRARSADPFEPPIIQTNYLAEELDRRTIVAGMKLARQLLKSDPLAPYYAHEDFPG
                     PDVQTDDEFLAAATQRGTTTFHPGCTCRMGPAGSSWAVVDDQLRVHGLQNLRVIDASI
                     MPRMISANLNASTMMIADKASDLIRGRKPPEAATVIATGV"
     gene            complement(7683..>8276)
                     /locus_tag="VN968_09460"
     CDS             complement(7683..>8276)
                     /locus_tag="VN968_09460"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HXL29497.1"
                     /translation="MPWLEPISLRGPHARLEPLSQDHCEGLTEAVKDGELWKLWYTFI
                     PRAEDMGKEIDRRLGLQAAGSMLPWTVFDADGRIAGMTTYMNVDAPNRRVEIGSTWYA
                     TRVQRSALNTQCKLLLLDHAFENLDCIAVEFRTHFFNHQSRRGIERLGAKLDGILRSH
                     QIASNGTLRDTVVYSIIAGEWPTVKAHLTYQLNEKAR"
BASE COUNT         1608 a         2597 c         2563 g         1508 t
ORIGIN      
        1 cgatctcccg ggggtcgcgg atgtcgacga tcacgacgtc gtcgctctgc gcggccttga
       61 tggcctcggt cgcgctcaac gtctcgatct cggcattggc ctcgtcgatc agcgccttga
      121 tgccgcggtg gatggtctgg ggcattttat catacctctc acttccgtca ttccggggcg
      181 atgccaacgg gtccgcgcaa agcgcggccc gatgacaggc tccgcatcga acccggaatc
      241 tcgagattcc gggttcgcgc tgcgcgcgcc ccggaatgac gacgacaact accgcaccag
      301 ttccttcatc gcgccttcaa gaccttcgat ggtgatcggg aacatgcgct ccgaaaacaa
      361 tcggcgaatg agggtggtcg attccgaata gtcccagtgc cgctgcgcca ccggattgag
      421 ccacaccgcg tgcggatagg tccgcgtgat gcgttcgagc cagaccgatc ccgcttcttc
      481 attgacatgc tcgaccgagc cgccgggcac catgatctcg tatgggctca tcgaggcgtc
      541 gccgacgaac accaccttgt agtcgtgcgg atatttgtgc agcacgtccc aggtcggggt
      601 gcggtcggtg aagcggcggc ggttctgctt ccagacgcct tcatagaggc agttgtggaa
      661 gtagaaatat tccatgtgct tgaattcggt cttggccgcc gagaacagtt cctcgacctg
      721 ctcgatatgc gaatccatcg agccgccgat atcgaagaac accagcacct tgaccgcgtt
      781 gcgccgctcg gggcgcatat gcacgtcgag atagccgtgg ttggcggtct ccttgatggt
      841 ggtgtcgaga tcgagttcgt caggcgcgcc ggtgcgggcg aatttgcgca ggcgtcgcag
      901 cgcgatcttg atgttgcgga tgccgagttc gacgttgccg tcgaggtcct tgaactcgcg
      961 gcggtcccac accttcaccg cgcgatggtt gcggttcttt tcctgaccga tgcggatgcc
     1021 ttccggattg tagccctccg cgccgaacgg cgaggtgccg gccgtgccga tccatttgtt
     1081 gccgccctga tgccggccct tctgctcctt caagcgctgg cgcaaggtct ccatgagctt
     1141 gtcccagccc atcgcctcga tctgcttttt ctcttcctcg gtgaggtatt tttccgcgag
     1201 cttcttcagc cattcggcgg gaatgtccgc cttctccatg gcgtcgagca ggctctccag
     1261 ccccttgaac acagtgccga acacgcgatc gaacttgtcg agattgcgct cgtcctttac
     1321 cagcgcggtg cgcgcgaggt agtagaaatt ctccaccgtc tgctcggcga ggtcggcgtt
     1381 gagcgcctcc atcagcgtca gatattcgcg caaggtcacc ggtacctggg cgtcacggag
     1441 cgatgtgaag aattgcaaaa acatgagcta cagattgccc gccgggccgt gaacgtcaag
     1501 ggcttccgcc tgccagacag gcctagacct gccgggtttt tcaactagaa ttgaaccagc
     1561 aaggcttttt cacaacgacc gccgatccgg cggtcgtttc ctgttcagac gcgcccgcgc
     1621 gcaagaataa caacaaggca aatactcgat gaagtttacc ggcaccaagg actacgtcgc
     1681 caccgacgac ctcaagatcg ccgtcaacgc ctcgatcgtg ctcgagcgac cgctgctgat
     1741 caagggcgag cccggcaccg gcaaaaccgt gctggctgag gaagtcgcca aggcgctcgg
     1801 tgcgccgctt ttgacctggc acatcaagtc caccaccaag gcgcagcagg gtctgtacga
     1861 atacgacgcg gtgtcgcgct tgcgcgacag ccagctcggc gatgcccggg tctccgatat
     1921 ctccaactac atcaggcgtg ggaaattgtg ggaagccttc acccacgaga agcgcccggt
     1981 gcttctgatc gacgagatcg acaaggccga tatcgaattt cccaacgatc tgttgctcga
     2041 actcgatcgc atggagttct tcgtctacga gaccggcgag aacatcaagg caaagctgcg
     2101 cccgatcgtg atgatcacct cgaacaacga aaaggaattg ccggacgcgt tcctgcgccg
     2161 ctgtttcttc cactacatca aatttcccga catcgacacc atgaaccgga tcgtcgacgt
     2221 gcattttccc ggcatcaaga aacgcctggt ggaggaagcc ttgcggatct tctttgaagt
     2281 gcgcgaagtg ccgggcttga agaaaaagcc ctcgacgtcg gagctgctgg actggctgaa
     2341 actgctgctc aacgaagaca tgacgccgga aatgctccgg gagcgcgacc cgcgcaagct
     2401 gatcccgccg ctgcacgggg cgctgttgaa gaacgagcag gacgttcatc tatttgagcg
     2461 cctcgccttc ctcagccgaa gagaggtgtg aggacggccc gcttcgcacc gcggcaatcg
     2521 aattaagaaa tagaacaata aaaatagcag ggatcgggag atgaacgttc aacagcagat
     2581 ccgcgcagcc tccgccgaca gcaccgagca tttcggtgtc gtaatcgccg gcgcgggaat
     2641 ttccggggtc ggtgcggcct accatctgac caagcagtgt ccggggacaa gttttgtcgt
     2701 gctcgaaacg caagcgagtt tcggcggaac ctggcgaacc caccgctatc ccggaatccg
     2761 ctcggacagc gacctgcata ctttcggcta ccggttcaag ccgtggacca gcgccccgat
     2821 cgcgaccgca gccgagattc tcagctacat gggcgaggtg atcgaggaaa acggactcgg
     2881 ccgtcacatc cgctatcggc acaggatttc ctcggcgcgg tggtcgagcg aggaaaatct
     2941 ctggaccatc gaagctgtta ggaccgatag cggcgaagcg gtacgcttca ccgcgaactt
     3001 cttctggatg tgccagggct attaccggca cgcggaaggt tatacgccgg agtggaaggc
     3061 catggagacc ttcaagggcc ggatcgttca cccgcagcaa tggcctgagg atctcgacta
     3121 caaggacaag agggtcgtcg tgatcggctc cggcgccacc gcggcgacgc tgattccggc
     3181 tatcgccaat gattccgccc atgtgaccat gctgcagcgc tcgccgacct attttcggac
     3241 cggtcgcaac gcgatcgaca tcgccgagac cctgcggacg ctgcaggtcg acgagacctg
     3301 gatccatgaa atcacgcgcc ggaaaattct ctacgatcag gccgagttca cccgcaagac
     3361 cttcgaagaa ccggaagcgg ccaagaagga cctgctgtcc gccgtcgaag cctatctcgg
     3421 ccccgattac gatatcgcga cccacttcac gccaaaatac cggccgtggc ggcagcgcat
     3481 cgccttcatt ccggacggcg atctgttcca gggcatcaag tccggcaagg cctcggtcgt
     3541 caccgacgag atcgaccgct tcaccgagac cggcatcctg ctgaagtccg gcaagacgct
     3601 cgaagccgac atcatcgtca ccgcgaccgg cttcaacctg agtgccaacg gcgacatgga
     3661 atttggggtc gacggcaagc cgctggattt cgccgacacc gtgacctatc gcggcatgat
     3721 gttcaccggc gtgcccaacc tcgtctgggt gttcggatat ttccgggcga gctggacgct
     3781 gcgggtcgat ctggtcgccg attttgtctg tcggctgctc gaccatatga agcaaacagg
     3841 ggcaaggaag gtcacgcccg ctatgcgcgc ggaagaccac aacatggcgc tgttgccgtg
     3901 gattgatcct gaaaatttca accccggcta catgatgcgc ggcatgcatc tgttgccgaa
     3961 gcgcggcgac aagccggaat ggcaacacaa ccaggattac tgggccgaaa aggacgagtt
     4021 tccggccatc gacctcaacg atcgcgcgtt cgtttacgag tgaacggagc gagcggcgcc
     4081 gtatcagcga tccgtccgga gcgacgccat catggacgtg atcgcaagca ggataatgac
     4141 ggtgccgtag agataggcgg cggcggagag accgatcagc gggaccgata atcctgccgc
     4201 gaccgccggc aggctgaacg ccagatagga ctgcacatag aacgcggaca atagcccggc
     4261 ccgctgatcg ggctcggcgg tgggcagcaa ggcgcgcaat gtgccggaga atgtcgaccc
     4321 gaatcccgcg ccggcgatca cggtcccggc cagcaatgct gcgacctgct gctgctggat
     4381 tccgaacatc gagaccgcca ccccgagcga tagcgtggcg gtgcctgtca agatcagacg
     4441 ccgcgccggc cattccctga aggccgccac cgcgatggcc gccgtcaaca tcagcgccga
     4501 taccacgacg ccgccgatcc agggcgagac gacgcccatc gcggtggcca ccacggtcgg
     4561 catcagggag aggtagagtc cgccgagtgc ccaggccgcc acattggccg gtgtgagcct
     4621 gatcagaacg gacctcgact ggcgcggcac gccgatttgc ggtcgcagcg acgccagggc
     4681 cccggccttt cgcggcgtgc tctcgggcat gacccagagc agcgcgatca tcagcgccgt
     4741 caggccaagc agaacctcat agaccctgtg cagcgggtcc ggcgcgaacg tgatcagtgc
     4801 cgccgcgccg agcgaaccgg ccgtcatccc cagaaacgcg gtgacgctgt tgagcagagg
     4861 gccccgcgcg cgattggtat cgaggatggc ggcgcccaac gccgtcgtgc cggtgccgac
     4921 gcaaagtccc tgcaccgccc gcgccagaat cagttgcccg acatcgcggg cttccgcgaa
     4981 cagaagcatc gccgcggcgt tcagcagcag cgccgccagg atcaccggcc ggcgccccac
     5041 ataatcggat aatccgccga ccgtcagcag gccggccagc aggctcgcga catacaccgc
     5101 aaagacgatc gtgatccaga acggggtgag atgcatcgac tgctgataga gccggtacag
     5161 cggcgtcgcc gcgctgctgc tcgccgcgag aagcatcgcc ccgccgaagc tgaacagcgt
     5221 catggcgcgc ggtggcaggg agcggggttg ggatagtgcg ggaagtactg tggcgcgggc
     5281 ttcggacggc ggattggccg gacaggcttg catgtcgggt tcctaaagct aatatatagc
     5341 cttagcgata tagggtggag aaccccttaa agcaacttat ttgcgttaag ccgaaccacc
     5401 gttttggcat ggcaaggttg cacggagcaa aagcgtggcc gtcaaagaac gcgttcgcgt
     5461 cgggggacgc agcgcccgaa ttcagagcgc cgtgcacgag gcggtgagaa atctcagcct
     5521 caaaaccaac cgcgatgaac tcacggtgcc tcagatcgcc gccgaggccg gggtgacacc
     5581 gtcaacgatc tatcggcggt ggggcgacct ttcagcgctg ctggcggatg tcgccgtcca
     5641 gcggttgagg ccgatcgcgg atcccgagga caccggcgcg gtggcgtcag atctgcgggt
     5701 gttcatcgag caattcatgg aagagatgtc ctctccagtc ggccgggcgt tgatcaggga
     5761 tgtgttctcc ccgccgggag agacataccc ggtccagtgc gcgggcttta cctacgaaca
     5821 tctcaagacc atcgtttcgc gggcgaaagc gcgcggcgaa ccgcctttcg atatcgatga
     5881 ggtgatcgac cacgtcgtcg cgccgatcat ctatcacatc ttgtacggca accgggaact
     5941 gacgctggac tattgccatt gcctgttgga ccgccttcaa tcgcttaacc cgaagtatta
     6001 ggtcgttcaa aaaacatctc agacgccggt cgcaatcaca gtagcggcct ccggcggctt
     6061 gcgaccacga atgagatccg acgccttgtc ggcgatcatc atggtcgagg cattgagatt
     6121 ggccgagatc atccgcggca tgatggaggc gtcgatcacg cgcaggtttt gcagcccatg
     6181 gacccggagc tggtcgtcga ccaccgccca gctgctgccg gccgggccca tgcggcaggt
     6241 gcatccgggg tgaaacgtcg tggtgccgcg ctgggtcgcc gcggcgagga attcatcgtc
     6301 ggtctgcacg tcgggtccgg gaaagtcttc gtgcgcataa tagggcgcga gcggatccga
     6361 cttgagcagc tgccgcgcca gcttcatgcc ggcgacgatg gtgcggcgat cgagctcctc
     6421 ggccagataa ttggtttgaa tgatcggcgg ctcgaacgga tcggccgagc gggcgcggac
     6481 atagccgcgg ctttccggcc gctgctgcca ggatgcgacc gtcatgccgg gttcgttttc
     6541 gagctggccc tgcacgccct ccttgtagct cgcgggcgta aacgtcagct gcagatcgga
     6601 gctctcggtg gtctcgccgg agtgccagaa gcaatagacc atggtcggcg acagcgacag
     6661 caggccgcgg cgcgtcgtgg cccatttgat cgcctccacc gcaagactga ggccgcgccg
     6721 gcgctcattg atggtcttga tgttcttgac gcgggcgacc gaacgcggcg cgtaatggtc
     6781 ctgcaatccc tcgccgacac cgggcagcgc gtggcgcacc gcgatgccga gcgaggccag
     6841 cagttccggc gatccgacgc ccgacaactg caaaagctgc ggcgagttgt aggtgccgcc
     6901 cgccaaaatg acttccctgg ccgcgcgcac ctcggtgaca gcaccgccct tgccgccctt
     6961 ggcgtagcgc acaccgattg cgcgcttgcc gtcgaacacg atctcggtcg catgcgcgtg
     7021 cgtccgcaca tggacattcg gccgtttcat cgccggatgc aggaacgcgg tcgccgcact
     7081 gacgcgcagg ccgtttttga tggtgcgctg ggcgtaggat acgccctcct ggatcgcgcc
     7141 gttgtaatcg ggattgcgcg gaatgccgag gctcattgca ccggccatga acgcctcgca
     7201 gagcggatcc cgccattcca tggtggtgac ggtcagattg ccgtcgcggc cgcgaaaggc
     7261 gtcctcgccc tcacccacgc gccgctcgag ccgcttgaaa tagggcaaca cgtccggata
     7321 gccccagccg cgattgccga gctgcgccca ggtatcgaaa tcctggcgct ggccgcgatt
     7381 gtagatgtgg ccgttgatcg aagaggagcc gccgagcgtc ttgccgcgcg gcgcatagat
     7441 gctgcggccc ccggtccagg gacccggctc ctgctggtac gcccagttga tgcgcttcat
     7501 gtgaaaggtc ttgatgaagc ccgccggcag atggatgtag ggatgccagt cgcgaggtcc
     7561 ggcttcgagc acgcagatgc tgttgccctg gtcctcgctc agccggttgg tgagcacgca
     7621 tccggcggag cccgcgccca cgatcacata atcgaatgta tccatcatct tccgccggtc
     7681 cgctaccgcg ccttctcgtt caactgatag gtcagatgcg ccttcaccgt cggccattcg
     7741 ccggcgatga tgctgtagac cacggtatcg cgcagcgtgc cgttcgatgc aatctgatgg
     7801 ctgcgcagga tgccgtcgag cttggcgccc agccgctcga tgccgcgccg gctctgatga
     7861 ttgaaaaaat gcgtgcggaa ttctaccgca atgcaatcga ggttttcgaa cgcatggtcg
     7921 agcaacagca atttgcattg ggtattgagc gcgctgcgct gcacgcgcgt ggcataccag
     7981 gtggaaccga tctcgacgcg ccggttcggc gcatcgacgt tcatgtaggt ggtcatgccg
     8041 gcgatcctgc cgtcggcatc gaaaaccgtc catggcagca tggagcctgc cgcctgcagc
     8101 cccagccggc gatcgatctc cttgcccatg tcctcagcgc gcggaatgaa ggtgtaccag
     8161 agcttccaca gctcgccgtc cttcacggcc tccgtcagcc cctcgcaatg gtcctgcgac
     8221 aacggctcaa gccgcgcatg cggaccgcgt agcgatatcg gttcaagcca gggcat
//
LOCUS       DATNOI010000248        13948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2033811,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000248 DATNOI010000000
VERSION     DATNOI010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13948
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033811"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VN968_09465"
     CDS             complement(<1..548)
                     /locus_tag="VN968_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXL29498.1"
                     /translation="MISKFFIERPVLSNVIAILMILIGGVSLFSLAVAQYPDIVPPTV
                     QVTTRYPGASAKTVIDTVALPIEQQVNGVEDMLYMQSYSGADGTYSLTVTFKIGTDLN
                     FAQVLVQNRVSSALSQLPQSVQNQGVTVQKRSTAILLFVTLTSPNKTYDSLFLSNYAT
                     INIRDELSRLPGVGNVTVFGAG"
     gene            complement(557..1756)
                     /locus_tag="VN968_09470"
     CDS             complement(557..1756)
                     /locus_tag="VN968_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXL29499.1"
                     /translation="MKPTHSLTSRLNGRARRRAAAVSRIAVTILLFVVLSACEQNSFV
                     PPPPPKVEVGVPIKRTVTRFLEATGNTAPIKSVDLVARVQGFLQSINYQDGSFVKQGT
                     TLFTIEPETYKLKLEQAQAAEVGAQATVKQSEADFKRQMELVQKQAVSQSTLETSTST
                     RDNAQASLQQAQANTKIAAVNYGYTNVVAPFDGIVSAHLVSVGELVGAASPTQLATIV
                     QFDPIYVNFNVNEQDVLRIRTEAVRQGLTADDLRQVPVEVGLQNETGYPHKGKLDYAA
                     PTITQSTGTLAVRGVLPNSDRVLLPGYYVRVRVPTDRQEGALLVPDVALGSDQAGRYV
                     LVVNGDNVVEQRKVQLGPLEGDLRVIESGLKPDDRVVTAGLLRAIPGQKVDPQLQKIE
                     TSAAPAK"
     gene            1945..2766
                     /locus_tag="VN968_09475"
     CDS             1945..2766
                     /locus_tag="VN968_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29500.1"
                     /translation="MVSMLSRTAALLAWIATVIFAVAMPAFSQAPTDAQREAIRSECR
                     SDYEAHCASVPPGGAASLQCLQKSMSSLSSPCQTAVRAVGAPAAPKAESAPAAPKAES
                     AAPATTAKPATESAAKAATTTAKKPGDAQVAAIRGACRSDYPKVCAGVPTGGAPALQC
                     LEKNKSKVSANCEKAISAASGGAAAAGAAGAPAATATAAPATAPAATAAPALVLRPLR
                     PREEIFVLRSACGGDVRSLCGGVQPGGGRIVQCLATRAASLSPACKEVLSQFAAR"
     gene            3041..4843
                     /locus_tag="VN968_09480"
     CDS             3041..4843
                     /locus_tag="VN968_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL29501.1"
                     /translation="MLEKAPNGKITSLLNALDETLSAGDVDRAADLFQTDCYWRDLVT
                     FTWNIKTMEGKEQVRDMLKARLADTKPSNWKIADGEDASEADGITESWIQFETGVARG
                     FGHIRLKDGRIWTLLTTMSELKDHEEPLGFDRPMGAKHGAERNRTTWKEDREAEATEL
                     GYSRQPYCVIVGGGQGGIALAARLRQLNVPTIIVEKNEKPGDSWRKRYKSLCLHDPVW
                     YDHLPYLPFPRNWPVFSPKDKIGDWLEMYTKVMELNYWGSTECKKASYDEKSREWTVV
                     VQRDGKEIVLKPKQLILATGMSAKPNMPKFEGMDIFKGDQHHSSQHPGPDKYRNKKAV
                     VIGSNNSAHDICAALWEAGADVTMVQRSTTHVVRSDSLMELGLAALYSEQAVQSGMTT
                     AKADLIFASLPYKILHEFQIPVYNAIRERDADYYKRLEKAGFLLDYGDDDSGLFMKYL
                     RRGSGYYIDVGASELIANGSIKLKSGVDVKKLTEHSVILSDGTELPADLVVYATGYGS
                     MNGWAADLISREVADKVGKVWGLGSNTTKDPGPWEGEQRNMWKPTQQEGLWFHGGNLH
                     QSRHYSQFLSLQLKARMEGIPTPVYGLQKVHHLS"
     gene            4998..5678
                     /locus_tag="VN968_09485"
     CDS             4998..5678
                     /locus_tag="VN968_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007814007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HXL29502.1"
                     /translation="MFRSRHLLPLAALAVALACGAPAPAQNIVDEWSSVKPPPPPELK
                     PVTLDPKTTALLAMDLIKQFCNTELRPRCVASIPRIEKLLADARAKGVTIIYTLVPTA
                     GPNVPAPAIGDTLPAVAPKGNEPVIVALVDKFMLGDKDTGLANILKDKGITTVIAVGT
                     AAHGAVLYTASTAALRGFNVIVPVDGMSTVGQDTYVEQYVAYNLTHAPILSPKITLTR
                     TDMISFSN"
     gene            5733..6884
                     /locus_tag="VN968_09490"
     CDS             5733..6884
                     /locus_tag="VN968_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXL29503.1"
                     /translation="MRIAVIGGGPGGLYFAYLWKRRHPDARIDLFEQNRAGATWGFGV
                     VFSEQALEFLRTDDPDTVDVIAPRMESWNNITLNLRGESVEIDGVGFSSIGRLELLGI
                     LQERASSVGVALRYDTLIASVDQLTGYDLIVGADGLNSLIRRSFEGDFGTSLSYSANK
                     FAWYGTSKRFETLSQTFVETGLGSFNAHHYRYSPDMSTFLVECDLATWQRYGFANRTV
                     EQSKAICEQVFASTLDGDSLVSNKSVWRNFPWIWNERWSFKNMVLIGDALHSAHFSIG
                     SGTRLAIEDAIALTRALEAERDIAAGLARYQTGRKPIVKKLVTAARTSADWYEKFPEH
                     MKLGLMDFAYSYITRSGRIDDARLRAMSPAFMAKYAASRQSLRQSGGKG"
     gene            6881..8515
                     /locus_tag="VN968_09495"
     CDS             6881..8515
                     /locus_tag="VN968_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011082939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HXL29504.1"
                     /translation="MTSPKADTALEIADAVPPDSPGALEIGFAIPQQYNASRILFDNL
                     SRGWGGRLALTGPTGERSYAELCAEASRWGHGFQSLGLGRGDRVLMFLDDTPAYPAAF
                     FGAVRAGLVPLLTNTLTTPDLLQFYLSDSGATAAVADAEFCARFDATACKDTALRTLI
                     VINGAAAQHAVPNTVIAEQWLRGFPAELAEADTHRNEMAFWMYSSGSTGRPKGIVHLQ
                     HDMAYTEAAFGRNVLKLRDSDVCFSVPKIFFAYGFGNSITFPFSAGAATLLLPGQPKP
                     AAIFAAIERFRPSVFFGLPTLYTSLTKADGAAATDFSSLRMALSAAEVLSAEVFNAWK
                     LLSGLGIVEGLGSTEELHIYLSNRPDRKKVGAAGLRVPGYEIALKDKDGREVGDNEEG
                     IMWVRGDSSTPLYWNRPDKSAETIRDGGWIYTGDRFVRDSDGFYFFRGRADDLVKISG
                     QWVYPLEVELCLAEHPDVRECVVFALELPDRRMTLKAVVVMNSRTFDAGEATTMLQDF
                     VKANLLPYKYPREIKFIDELPKTGTGKIDRQAVMKM"
     gene            complement(8669..9862)
                     /locus_tag="VN968_09500"
     CDS             complement(8669..9862)
                     /locus_tag="VN968_09500"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HXL29505.1"
                     /translation="MASPGARAVLIAGGGVGGLAVALGLAQKGIRSILLEKASALGAI
                     GAGIQLGPNAFHAFDYLGVGEAARGMAVYIDQLRLMDALTAEEITHVDLREAFRARFG
                     NPYAVVHRGDLHGVFIKACRDHDLIDLRVSSEVIGYDQDGASVTARLGSGERVIGSLL
                     IGADGLWSNIRKQVAADGPPRVSGHTTYRSVIPTEQMPEDLRWNAATLWAGPKCHIVH
                     YPLSGWKVFNLVVTYHNDAPEPVVGKPVSDDEVMQGFGQVHPRAQDIIRHGKNWRLWV
                     LCDRDPGPRWVDGRVALLGDAAHPTLQYFAQGACMALEDAVCLSHMLGAYPDDHAAAL
                     ERYRSQRFPRTARVQLQSRLIGEHIYHPAGEHARIRNAIMRAKTSEDYYNDLAWLYGG
                     TGLAG"
     gene            complement(9862..10371)
                     /locus_tag="VN968_09505"
     CDS             complement(9862..10371)
                     /locus_tag="VN968_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXL29506.1"
                     /translation="MQNKSAPLTMDAVYAKPGYLFRRMQQIAVAIFVEECKAYDLTPV
                     QYAALVAIRTHPGIDATRLSAVIAFDRSTLGNVIERLEAKQLIERKPSREDRRVKLLY
                     LTRAGGALLRDIMPSVDRAQARMLQPLKPDDRRTLMALLTQLVDLNNEASRVPLRAED
                     ALEHLGRSS"
     gene            complement(10361..10993)
                     /gene="maiA"
                     /locus_tag="VN968_09510"
     CDS             complement(10361..10993)
                     /gene="maiA"
                     /locus_tag="VN968_09510"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HXL29507.1"
                     /translation="MKLHGYFRSSAAYRVRIALNLKGLSAHHLPHHLRKGEQRDPAYL
                     AINPQGLVPTLQDDAGAIITQSLAIIEWLEETHPEPPLLPKDRLRRAKIRAFAMALAC
                     DTHPVQNLKVLARLRELGLPEEQVTGWAAWANREGLAACERLIANEPGPFCFGAAPTM
                     ADLCLVPQLGNARRFGVDVHAFLRLLEAEAAAKNIKAFVDAAPERQPDAE"
     gene            11105..12145
                     /gene="gtdA"
                     /locus_tag="VN968_09515"
     CDS             11105..12145
                     /gene="gtdA"
                     /locus_tag="VN968_09515"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HXL29508.1"
                     /translation="MEAVQKTPEREAFYRKIDGENLSALWNVMGELITPEPKSACRPH
                     LWRFDAIRDYMTEAGKLITAKEAERRVLVLENPGLRGQSKITTSLFAGVQMVIPGDVA
                     PAHRHSQSALRFVLEGKGAYTAVDGERTAMEPGDFVITPSMTWHDHSNETSEPMFWLD
                     GLDIPMVQFFDASFAEGSNEDQQKLTRPAGDSFARYGHNLLPVDEKRASKTSPIFNYP
                     YSYTREALEQAKVRNEWDACHGLKLKFSNPETGDFAMPTIGTFIQLLPKGFETARYRS
                     TDATVFAAIEGKGRSRIGEQTFEWGPRDLFVAPSWHWVTHEAEDDSVLFSFSDRPVQQ
                     KLDLFREDRGNA"
     gene            complement(12726..13562)
                     /locus_tag="VN968_09520"
     CDS             complement(12726..13562)
                     /locus_tag="VN968_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL29509.1"
                     /translation="MLDRADDPAASPQAHADTQPRPVTFRGAGFVPGAGRYSGWIALV
                     LAIGFWQLAGSAALVNPLFLPAPSAIAVAIYKLALSGALWHHLSVSVMRIGTGWILGT
                     VAGVIVGFPIGLSSLARGVGITFISALFPIPKIALLPLLILWLGIGEEPKIATIALGV
                     FFSTAISVYSGVDNVPRNLIRMAQSFNVPFHSIVRRVIWPGALPSILAGFRITASVAL
                     LLVVSAEMIGAEYGIGSFVLQAGNLMQTDQLLAGVVILSLFGLAVGKLINLLETRLLH
                     WR"
     gene            complement(13555..>13948)
                     /locus_tag="VN968_09525"
     CDS             complement(13555..>13948)
                     /locus_tag="VN968_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL29510.1"
                     /translation="KQLSGGMRQRVGIARALAVRPAILLMDEPLSALDSQTRELLMED
                     FIGLLADGSMGAVYVTHNLEEAVRLADRIVVLSRRPGRIREVVTIPLTRGERGDITAR
                     AKLLALQSELWSLIREQAIDAEREVQHA"
BASE COUNT         2707 a         4384 c         4328 g         2529 t
ORIGIN      
        1 tggccggcgc cgaacacggt gacgttgccg acgccgggca accgcgacag ctcgtcgcga
       61 atgttgatgg tggcgtagtt cgacaggaac aggctgtcat aggtcttgtt cggcgacgtc
      121 aatgtcacga acaacaggat cgccgtggac ctcttctgca cggttacgcc ctggttctgg
      181 accgattggg ggagctgcga cagcgcgctc gacacccggt tctgcaccag cacctgcgcg
      241 aagttgagat cggtaccgat cttgaaggtc accgtcagcg aataggtgcc gtcggctccg
      301 ctgtaggact gcatatagag catgtcctcg acgccgttga cctgctgctc gatcggcagc
      361 gccacggtgt cgatcacggt cttggcgctg gcgccgggat aacgcgtggt gacctgcacc
      421 gtcggcggca cgatgtcggg atattgcgcg accgcgagag aaaacagcga caccccgccg
      481 atcaggatca tcaggatcgc gatgacgttg gaaagcaccg gccgctcgat gaagaatttc
      541 gaaatcatgg ccggtcctat ttggctggcg ctgccgaggt ttcgatcttc tgcagttgcg
      601 gatcgacttt ctgccccggg atcgcgcgca acagcccggc ggtgacgacg cggtcatccg
      661 gtttcaggcc gctttcgatc acgcgcaaat caccttccag cgggccgagc tgcaccttgc
      721 gctgctcgac gacattgtcg ccgttcacga ccagcacata tcgtccggcc tgatcgctgc
      781 ccaacgcgac atcggggacc agcagcgcac cctcctgccg gtcggtcgga acgcggacac
      841 gcacgtaata tccgggcaac aacacacgat cggaattcgg cagcacgccg cgtaccgcca
      901 gcgtcccggt cgactgggtg atcgtcggcg ccgcgtagtc gagcttgccc ttgtgcggat
      961 agccggtttc gttctgcaac ccgacctcga ccggcacttg cctgagatcg tccgcggtca
     1021 acccctgccg cacggcttct gtccgaatgc gcaggacatc ctgttcgttg acgttgaaat
     1081 tgacgtagat cggatcaaac tgaacgatgg tggcgagctg ggtcggcgac gctgcgccga
     1141 cgagttcgcc gaccgagacg agatgcgcgc tgacgatgcc gtcgaacggc gcgacgacat
     1201 tggtgtagcc gtaattgacc gctgcgatct tggtgttggc ctgggcctgc tgcagactgg
     1261 cctgggcgtt gtcgcgggtg gacgtggagg tttcgagcgt cgactgtgag accgcctgct
     1321 tttgcaccag ctccatctgg cgcttgaagt cggcttcgct ctgtttcacc gtcgcctgcg
     1381 cgccgacctc cgcagcctgc gcttgctcga gtttcagctt gtaggtctcg ggctcgatcg
     1441 tgaacagcgt cgtgccttgc ttcacgaagc tgccgtcctg gtagttgatc gactgcaaga
     1501 acccctgcac ccgcgcgacc agatcgacac tcttgatggg ggcggtgttg ccagtggcct
     1561 cgagaaaacg cgtgacggtc cgctttatcg gcaccccgac ctcgaccttc ggtggcggcg
     1621 gcggcacaaa gctattttgc tcgcacgcgc ttaaaacgac aaacagcaat atggtgacag
     1681 caattcgcga aactgccgcg gctctccgcc gcgctctgcc attcaacctg ctggtcaacg
     1741 aatgcgtagg cttcattccc acatgccaat tcattttgtt ccagagaagg gttttcggag
     1801 agcggttttc gccgactatg aatagcaaca atccacttgc agtgaaaccc gaaagagaaa
     1861 atgatccccg ctcgcgaaga tttgtatctc cctgctgaaa atttcgcttc ggctgcagcg
     1921 cgacaacgag aaggatttct tcaaatggtc agcatgctga gccggactgc ggctctcctg
     1981 gcgtggattg caaccgtcat attcgctgtc gcaatgccgg cattttcgca agcgccgacc
     2041 gatgcgcagc gcgaggccat ccggtccgag tgccgctcgg actatgaggc ccattgcgcc
     2101 agtgttccgc cgggcggcgc ggcgtcgctg caatgcctgc agaaaagcat gtcaagcctc
     2161 tcctcaccct gccagacggc ggtacgcgcg gtgggagcgc cggctgcgcc caaagcggag
     2221 tccgcgccgg ccgcacccaa ggcagaatcg gctgcccccg caacaacagc caagccggcc
     2281 accgagagcg cagccaaggc agcgaccacg acggccaaaa agcctggcga tgcgcaggtt
     2341 gccgcgatcc gcggcgcatg ccggtccgac tatccaaaag tctgcgccgg cgtgccgacc
     2401 ggaggtgctc cggcactgca atgtctggag aagaacaagt cgaaggtttc ggcgaactgt
     2461 gagaaggcga tcagcgcggc gagcggtggc gctgctgcag ctggggcagc cggggctccg
     2521 gcagccacgg ctaccgccgc tcctgctacc gcccccgccg ctacggccgc cccggcgctg
     2581 gtgctgcggc cgctgcggcc gcgcgaggag attttcgtct tgaggtccgc ctgtggcggt
     2641 gatgttcgtt ccctttgcgg cggcgttcaa cctggcggtg gccggatcgt gcaatgcctg
     2701 gctacaaggg cagcctctct ctcgccggcc tgcaaggaag ttctgagcca gttcgctgcg
     2761 cgataggttc agacatcgcc acgttcgaca agaaggtgtt gaacgggtac gtcgctgccc
     2821 atgacccaga acaatcgcgc gcatgttgcg attagcccgc aattcagacc aacccggctg
     2881 tttcttgctc gcctctccgc ggcaacgtgc tagaattata tatcttgaaa gatcaatgac
     2941 cccgcgcgag cccgccgttt tcgttggcgc ctcgtttcag ctcgccggaa caaagaaaac
     3001 agcgcaggct ctcgccaccg gcgcaaggga ggacatcaag atgctcgaga aagccccgaa
     3061 cggaaaaatc accagcttgc tcaatgcgct cgacgagacg ctgtcggccg gcgacgtgga
     3121 ccgcgccgcc gatctgtttc agacggactg ctactggcgc gacctcgtga ccttcacctg
     3181 gaacatcaag acgatggagg gcaaggaaca ggttcgcgac atgctcaagg cgcggctcgc
     3241 cgacaccaag ccatcgaact ggaaaattgc ggacggtgag gatgcttctg aagccgacgg
     3301 gattacagag agctggatac aattcgaaac cggggttgcc cgcggcttcg gccacatccg
     3361 cctgaaggac ggccgaatat ggacgttatt gacgaccatg agcgaactca aggaccacga
     3421 agagccactt ggcttcgacc gtccgatggg cgccaaacac ggcgccgaac gcaatcgaac
     3481 gacgtggaag gaagatcgcg aggcggaagc gactgaactc ggctactcgc gtcaacccta
     3541 ttgcgtcatc gttggtggcg gtcagggcgg catcgcgctg gcagcccggc tacgccagct
     3601 caatgtgccg acgatcatcg tcgagaaaaa cgaaaagccc ggcgacagct ggcgcaagcg
     3661 ctacaagtcg ctctgcctgc acgatccggt ctggtacgat catctgcctt atctgccgtt
     3721 tccccggaac tggccggtgt tctcaccgaa ggacaagatc ggggattggc tcgaaatgta
     3781 tacaaaggtg atggagctca attactgggg ctcaacggag tgcaagaagg catcttacga
     3841 cgagaagagc cgcgaatgga ccgttgtcgt gcagcgcgac ggcaaggaaa tcgtcctcaa
     3901 gccgaaacag ctgatactcg cgacggggat gtcagccaag cccaacatgc cgaaattcga
     3961 aggcatggat atttttaagg gcgatcagca ccattcgtcg cagcatccgg ggccggacaa
     4021 gtacaggaac aagaaagccg tcgtgatcgg ctcgaacaat tccgcccacg atatttgtgc
     4081 cgcgctctgg gaagccggag cagacgtcac catggttcaa cgctcgacga cccatgttgt
     4141 gaggtcggac tcgctgatgg agctcggact ggctgcactc tactccgaac aggcggtgca
     4201 gtccggaatg acgacggcca aggccgacct gatctttgcg tcactcccct acaagattct
     4261 gcatgagttc cagatccccg tctataacgc gatcagggaa cgcgatgccg attactacaa
     4321 gcgtcttgag aaggcaggct tcctgctcga ttatggtgac gacgattccg gcctgttcat
     4381 gaaatatctg cggcgcgggt caggatacta catcgatgtc ggtgcgtcgg aactgatcgc
     4441 caatggcagc atcaagctga agagcggcgt cgatgtcaaa aaactcacgg agcattccgt
     4501 gattctgagc gacggcaccg agctgccggc cgatctcgtc gtatacgcca ccggctacgg
     4561 ttcgatgaac ggctgggccg cggacctgat ctccagggaa gtcgctgaca aggtaggcaa
     4621 ggtttgggga ctaggctcga atacgaccaa agacccgggt ccatgggaag gcgagcagcg
     4681 caacatgtgg aagccgacgc agcaggaggg gctctggttc catggcggta acctgcacca
     4741 gtcgcgacac tactcgcagt ttctgtctct ccagttgaag gcgcgaatgg agggcattcc
     4801 cacaccggtt tacggtcttc agaaggtcca ccatctcagt tgatcctcct acagggaggt
     4861 cacacgcaac ggcgcgaggt gttcgcacct tcagcaactc gccggcgagc tagttgaaca
     4921 ggtaattcgg ggtttcgccg ccttgacggc gcgcgcactt gctggtccct tggggtaaca
     4981 aacctgggga ggaaaacatg ttccggtcac gccacctgtt gccgctcgcc gcgcttgcgg
     5041 tcgccctggc ctgcggcgcg cccgcgcctg cgcagaacat cgtcgacgag tggtcgagcg
     5101 tgaagccgcc gcctccgccc gagctcaagc ccgtcacgct cgatcccaag accaccgcgc
     5161 ttctggcgat ggatctgatc aagcagttct gcaacacgga actgcgaccg cgttgcgttg
     5221 cttccattcc caggatcgag aagctgctcg ctgacgcccg cgccaagggt gttaccatca
     5281 tctataccct ggtccccact gcgggaccca acgttcctgc tcccgcgatc ggcgacaccc
     5341 tccctgcggt cgcgcccaag ggcaatgaac ctgtcatcgt cgcccttgtc gacaaattca
     5401 tgctgggcga caaggatacc ggtcttgcga atatcctgaa ggacaagggg atcaccaccg
     5461 tcatcgcggt cgggaccgcg gcccatggcg ccgtgcttta caccgcgagc accgcggcgc
     5521 tgcgcggctt caacgtgatc gtgccggtgg acggcatgtc gaccgtgggc caggacacct
     5581 atgtcgagca atatgtcgcc tataacctga cccacgcgcc gatcttgtca ccgaagataa
     5641 cgctgacccg gaccgacatg atcagcttct cgaactgaac accggctggc gcagcgctgg
     5701 cgatggcgcg gcgccgtcga gaggaggcga aattgcgtat cgccgtgatc ggcggcggcc
     5761 cgggcggcct ctatttcgcc tatctctgga agaggcgcca cccggacgct cggatcgacc
     5821 tgtttgagca gaaccgcgcc ggcgccacct ggggatttgg cgtggtgttc tccgagcagg
     5881 cgcttgaatt cctgcgcact gatgatcccg acaccgttga cgtcatcgcc ccaaggatgg
     5941 agagctggaa caacatcact ctcaatctgc gcggcgagag cgtcgagatc gacggcgtcg
     6001 gcttctcctc gatcggccgg ctcgagctgc tcgggattct gcaggagcgc gcatcctcgg
     6061 ttggtgtcgc actgcgctac gacacgctga ttgcatccgt ggaccagttg accggctatg
     6121 atctgatcgt gggagctgat ggcctgaatt cgctgatccg gcgcagcttc gaaggcgatt
     6181 tcggcacctc gctgtcctat tccgccaaca agttcgcctg gtatggcacg tcaaaacggt
     6241 tcgagacgct gtcgcagacc tttgtggaaa ccggactcgg atcgttcaac gcgcatcact
     6301 atcgatactc gcccgacatg agcacgttcc tggtcgaatg cgatttggcg acatggcagc
     6361 gctacggctt tgccaacagg acggtcgagc aatcgaaagc catctgcgag caagtgttcg
     6421 cgtccacgct cgacggcgat tccctggtat cgaacaaatc ggtgtggcgc aattttccct
     6481 ggatctggaa cgaacgctgg tcattcaaga atatggtgct gatcggcgat gccttgcatt
     6541 cggcgcattt ttccatcggc tcgggtacgc ggctcgcgat cgaggatgcg atcgcgctca
     6601 ccagggcgct ggaggcggaa cgcgacattg ccgcaggcct cgcccgttac cagaccgggc
     6661 gcaagccgat cgtgaagaag ctggtgacgg cggcgcgcac cagcgccgac tggtacgaaa
     6721 aatttcccga gcatatgaag ctcggcctga tggattttgc ctatagctac atcacccgct
     6781 cggggcggat cgacgatgcg cgcctgcgcg cgatgtcgcc ggcgttcatg gcgaaatacg
     6841 ccgcgtcgag gcaatcgttg cgccaaagcg ggggcaaggg atgacatcgc caaaggcgga
     6901 tacagcactt gagatcgccg acgccgttcc ccccgatagc ccgggggcgt tggagatcgg
     6961 ctttgcgatc ccgcaacaat acaatgccag ccgcatcctg ttcgacaatc tttcccgcgg
     7021 ctggggcggt cggctggcgc tgaccggccc gactggtgag cgcagctatg cggagctctg
     7081 cgccgaagcc tcgcggtggg gccacggctt ccagtcgctg ggactagggc gcggcgaccg
     7141 cgtgctgatg ttcctcgacg ataccccggc ctatccggcg gcgttcttcg gcgcggttcg
     7201 cgcagggctc gtgccgcttt tgaccaatac gctgaccaca cctgacctct tgcaattcta
     7261 cctttccgat tccggcgcca cggccgccgt ggccgacgcc gagttttgcg cgcgcttcga
     7321 tgccacggcc tgcaaggaca cggcgttgcg cacgctgatc gtgatcaacg gcgcggccgc
     7381 tcaacacgcc gtgccgaata ccgttatcgc cgaacaatgg ctgcggggct ttcccgccga
     7441 gcttgcggag gccgataccc atcgcaacga gatggcgttc tggatgtatt cgtcgggctc
     7501 gaccggacgc cccaagggca ttgtccatct gcagcacgac atggcctata ccgaggctgc
     7561 cttcgggcgc aacgtgctga agctcaggga cagcgacgtc tgcttttcgg tgccaaaaat
     7621 cttcttcgcc tatggttttg gtaactccat caccttcccg ttctcggccg gcgccgccac
     7681 gctgctgctg ccgggccagc caaagccggc ggcgatcttt gcggcgatcg agcggtttcg
     7741 cccaagcgtg tttttcggat tgccgacgct ctatacctcg ctgaccaagg ccgatggtgc
     7801 tgcggcaacc gatttctcat ccctgcgcat ggcgctgtcc gccgccgaag tgctttcggc
     7861 cgaggtcttc aatgcctgga agctcctcag cgggcttggg attgtcgaag ggctcggctc
     7921 caccgaagag ctgcacatct acctctccaa ccgcccggac aggaagaagg tcggggccgc
     7981 ggggcttcgc gtgcccggct acgagatcgc gctgaaggac aaggacggcc gcgaggtcgg
     8041 cgacaacgag gaaggcatca tgtgggtgcg cggcgattcc tcgacgccgc tgtactggaa
     8101 ccggccggac aaatcagcgg agaccatccg cgacggcggc tggatctaca ccggcgaccg
     8161 ctttgtgcgc gacagcgacg gcttttattt cttccgcggc cgcgccgatg acctggtcaa
     8221 aatctccggc caatgggttt atccgctcga ggtcgagctt tgcctcgccg agcatcccga
     8281 cgtgcgcgaa tgcgtggtgt ttgcgctcga gctgccggac cggcgcatga ctttgaaagc
     8341 agtggtcgtg atgaacagcc gcaccttcga tgcaggcgag gcgacaacaa tgctgcagga
     8401 ttttgtcaag gccaacttgc tgccctacaa atatccgcgc gagataaaat tcatcgacga
     8461 gctgccgaag accggcaccg gcaagatcga ccgccaggcg gtgatgaaga tgtagcggcg
     8521 tactgcttcc gtttatcgca accgtcatcg cccgcgaagg cgggcgatcc agtaatcgcc
     8581 ggcagtgatg atgacgaacg ctgctgatta ctggatgccc cgccttcgcg gggcatgacg
     8641 agcggggttg cacgcgcacg ctcaggccct atcccgccag ccccgtcccg ccatagagcc
     8701 aggcgagatc gttgtaatag tcttccgacg ttttcgcgcg catgatcgcg ttgcggatgc
     8761 gggcgtgttc gcctgcgggg tgatagatgt gctcgccgat caggcgggat tgcagctgca
     8821 cccgcgcggt gcgcggaaag cgctgtgaac ggtaacgctc cagcgcggcg gcatggtcat
     8881 ccggataggc gcccagcatg tgcgacaggc acaccgcgtc ctccagcgcc atgcaggcgc
     8941 cttgcgcgaa atactgcagc gtcggatgcg cggcatcgcc cagcagagcg acgcggccgt
     9001 cgacccagcg cggacccgga tcgcggtcgc ataataccca cagccgccag ttcttgccgt
     9061 ggcgaatgat atcttgcgcg cgcggatgca cctgcccaaa accttgcatc acctcgtcgt
     9121 ccgacaccgg ttttcccacg accggttccg gcgcgtcatt gtgataggtg acgacgaggt
     9181 tgaacacctt ccagcccgac agcggatagt ggacgatgtg gcatttggga ccggcccaca
     9241 gcgtcgccgc attccagcgc aaatcttccg gcatctgctc ggtcgggatc accgaccggt
     9301 aggtggtgtg accggaaacc cgcggcggcc cgtcggctgc aacctgcttc ctgatgttcg
     9361 accacagccc gtcggcgccg atcagcagcg acccgatgac acgctcgcct gaaccaagcc
     9421 gcgccgtcac cgacgcgccg tcctgatcgt agccgatcac ttcagagctc acgcgcagat
     9481 cgatcaggtc gtgatcccgg caagctttga tgaatacgcc gtgcagatcg ccgcgatgca
     9541 ccaccgcgta agggttgccg aaacgcgcgc ggaaggcctc gcgcaagtcg acatgggtga
     9601 tctcttccgc tgtgagcgcg tccatcagcc ggagctgatc gatatagacc gccatgccgc
     9661 gcgccgcctc gccgacgccg agatagtcga aagcatggaa cgcgttcggt ccaagctgga
     9721 tgcccgcgcc gatggcgccc aaggcggacg ccttttcgag aagaatggaa cggataccct
     9781 tttgtgcaag accgagcgcc accgcgaggc cgccgacacc accgccggcg atcaggacag
     9841 ctcgtgcgcc cggcgatgcc atcagctcga ccttccaagg tgttcgagcg cgtcttcggc
     9901 gcgcagcggc acccgcgacg cctcgttgtt gagatcgacc agctgcgtca acagcgccat
     9961 cagcgtcctg cggtcgtccg gtttcagggg ctgcagcatc cgcgcctgcg cgcgatcgac
    10021 cgagggcatg atatcgcgca gcagcgctcc gccggccctg gtcagataaa gcagttttac
    10081 ccgccggtcc tcgcgcgatg gcttgcgctc gatgagttgc ttggcctcca accgctcgat
    10141 cacgttgccc agcgtcgagc ggtcgaaggc gatcacggcg gaaagccgcg tcgcgtcgat
    10201 gccgggatgg gtccggatcg cgaccagtgc ggcatactgc accggcgtca ggtcgtacgc
    10261 cttgcattcc tccacaaaga tcgcaaccgc gatctgctgc atccgccgaa acagatagcc
    10321 gggcttggca tagacggcat ccatcgtgag aggcgcggac ttattctgca tcgggctgcc
    10381 gctccggtgc ggcgtccaca aacgccttga tgtttttcgc ggccgcttca gcttcgagca
    10441 ggcgcagaaa cgcgtggaca tcgaccccaa aacgccgcgc attgccaagc tgcggcacca
    10501 ggcagagatc ggccatggtc ggcgccgcgc cgaagcagaa tggcccgggc tcattggcga
    10561 tcaatctttc gcaggccgca agaccctcgc ggttggccca tgcggcccat cccgtcacct
    10621 gttcttcggg caggccaagt tcgcgcaatc tcgccagcac tttcaggttc tgcaccgggt
    10681 gggtatcgca ggcgagcgcc atcgcgaagg cgcggatttt ggcgcggcgc aaccgatctt
    10741 tcggcagcag cggcggttcg ggatgggttt cctccagcca ttcgatgatg gcgagcgact
    10801 gggtgatgat cgcgccggca tcgtcctgca gcgtcggcac cagcccttgc ggattgattg
    10861 cgagataggc cgggtcgcgc tgttcgccct tgcgaaggtg gtgtggcagg tgatgcgcgc
    10921 tcaacccctt caggttcaat gctattctga cccgataggc cgcgctgctg cggaaatagc
    10981 cgtgcagctt catcggaacc tccctctggt tctcgttgtt tgctttcatt gccgtcattg
    11041 acgctagcca tattgtaagt atgctgtcaa tctaccaacc aacaagaacg acgggaggcc
    11101 cgccatggaa gccgtacaga agacgccgga acgcgaggcg ttttaccgca agatcgacgg
    11161 cgaaaatctt tccgccttgt ggaacgtgat gggcgagttg atcacgccgg agccgaagag
    11221 cgcctgccgg ccgcatctgt ggcgcttcga tgcgatccgc gactacatga ccgaagcggg
    11281 caagctgatc accgccaagg aagccgagcg gcgggtgctg gtgctggaaa atcccgggct
    11341 gcgcgggcaa tcgaagatca cgacgtcttt gtttgccggc gtgcagatgg tgatcccggg
    11401 cgacgtcgcg ccggcgcacc gccacagcca gtcggcgctg cgtttcgtcc tcgagggcaa
    11461 gggcgcctat accgccgtcg atggcgagcg caccgcgatg gagcccggcg attttgtcat
    11521 tacgccgtcg atgacgtggc atgaccattc caacgaaacc tccgagccga tgttctggct
    11581 ggacgggctc gacatcccga tggtgcagtt cttcgacgcg tcgttcgcgg aaggctccaa
    11641 cgaggaccag cagaagctca cgcggcccgc cggcgacagc tttgcccgct acggccacaa
    11701 tctgctgccg gtcgatgaga agcgcgcttc aaaaacctcg ccgatcttca actaccccta
    11761 cagctacacc cgcgaggcgc tggaacaggc gaaagtgagg aacgagtggg acgcctgcca
    11821 cggcctgaag ctgaaattct caaatcccga aaccggcgat ttcgcgatgc cgaccatcgg
    11881 cactttcatc cagctgctgc cgaagggatt tgagaccgcg cgctaccgct cgaccgacgc
    11941 cacggtattc gcggcgatcg agggcaaggg gcgttcgcgg atcggcgagc aaacgttcga
    12001 gtggggcccg cgcgatttgt tcgtggcgcc gagctggcac tgggtcaccc acgaggccga
    12061 ggatgattcg gtgctgttca gcttctccga ccgcccggtg cagcagaagc tcgatttgtt
    12121 ccgcgaagac cgcggcaacg cgtgaggtgc ggcgtcgacc caggccgtca tggccgggcc
    12181 tgtccggcca tccacgtctt tttttgccgc gacactgtta agacgtggat gcccggcaca
    12241 aggccgggca tgacgaaacc cgtcattgcg agcgaacggg tcgcgcgaat gcgccagcct
    12301 gccgcccaag ggccctgatc ggaaaagagt aggttttagc cccatcgaga ggttaggatt
    12361 tggagtggcg ctcgcttttc tcaggaggtt cgaccatgac acggcacctt ttcttcgcga
    12421 tcaccctcat cggcaccgga gtgctcctca attcaccggc agaagcagtg acagccgcgc
    12481 gatgtgagga tcaagccgca aattgcgtgg gcagatgtgc caatcccagg ggtggggcaa
    12541 atcagaacaa atgcatgcgc aactgcgacc ggcaggtgac cagatgtttg ataggcacga
    12601 cgcaatatag cgcggcccgg cggtggtagg cggtgacggg caaccgctcc acgccgcttg
    12661 cgccgaccgg cgaaccggat cacggtgcga tagcccggac gctccttccg ggctacgaga
    12721 gcgggctaac gccaatgcaa cagccgcgtt tccagcagat tgatcaactt accgaccgcg
    12781 agcccgaaca gcgacaggat gacgacgccg gcgagcagct gatcggtctg catcaggttg
    12841 ccggcctgca gcacaaacga gccgatgccg tattcggcgc cgatcatctc cgcgctcacc
    12901 accaatagca gcgcgacgga agcggtgatg cggaatccgg caaggatcga cggcagcgcg
    12961 ccgggccaga tcacccggcg cacgatcgaa tgaaacggca cgttaaaact ctgcgccatc
    13021 cggatcaggt tgcgcggcac attgtcaacg ccgctataga ccgagattgc ggtggaaaag
    13081 aacaccccaa gcgcgatggt cgcgatcttc ggctcctcgc cgatcccgag ccacaggatc
    13141 aacagcggca atagcgcgat tttcggaatc ggaaacagcg ccgagatgaa ggtgatgccg
    13201 acgccgcgcg ccaggctgga gagcccgatc ggaaagccga cgatgacgcc ggcgaccgtg
    13261 ccgagaatcc agccggttcc gatgcgcatg accgaaaccg acagatggtg ccaaagcgcg
    13321 cccgacagcg cgagcttgta gattgcgacc gcgatcgccg atggcgccgg cagaaacagc
    13381 ggattgacga gagcggcgct gccggccagc tgccaaaacc cgatcgccag caccagcgcg
    13441 atccagccgg aatagcggcc ggctcccggc acaaaacccg caccgcgaaa tgttaccggc
    13501 cgcggttggg tgtcggcatg cgcttgcggc gaggccgccg gatcgtcggc gcggtcaagc
    13561 atgctggacc tcgcgctcgg cgtcgatcgc ctgctcgcgg atcagcgacc ataattcgct
    13621 ctgcagcgcc agcaatttgg cgcgggcggt gatgtcgccg cgttcgccgc gcgtcagcgg
    13681 gatggtgacg acctcgcgga tacggccggg ccggcgtgat agcacgacga tgcggtcagc
    13741 caaacgaacg gcttcctcga gattatgcgt gacatagacc gcgcccatcg aaccatccgc
    13801 aagcagaccg ataaaatcct ccatcagcaa ttcgcgggtt tgcgaatcca gcgccgacag
    13861 cggctcgtcc atcaacagga tcgcgggccg caccgccagc gcgcgggcaa tgccgacgcg
    13921 ctggcgcatg ccgccggaca attgtttt
//
LOCUS       DATNOI010000249        29710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_225327,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000249 DATNOI010000000
VERSION     DATNOI010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 29710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29710
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225327"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VN968_09530"
     CDS             complement(<1..1356)
                     /locus_tag="VN968_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HXL29511.1"
                     /translation="MPPGSGALPWAATIGALGVVYGDIGTSPLYALKEAAKAAAHGGP
                     LAPEAILGVVSLILWALILIISLKYALLILRADNRGEGGIVALLALLSARNARPGTWR
                     AQLLVVGLVGAALLYGDGAITPAISVLSAIEGLKVDAPSLAPAVVPLTIAILIGLFIV
                     QKKGAGFIGNIFGPVMLAWFVVIALLGVHGIVRAPGVLAALSPLYAFDLLIHEDFHIS
                     FAVLGAAFLAVTGGEAMYADMGHFGRVPIRLAWFIVALPALVLNYFGQAALLITDASA
                     LDHPFYQLAPDWLHYPLVAFATVATVIASQAIISGVFSLTQQSIQLGFLPRMHIIHTT
                     SHAMGQIYVPLVNWLLAAATLGAVIGFGTSDALAGAYGIAVSLLMAITTLLAALVAIQ
                     WGYSPVIVIAVNGFFFVIDAIFFAANSTKLFEGGWFPLLLAAVVAFLMLTWRSGVRLV
                     EK"
     gene            complement(1515..1775)
                     /locus_tag="VN968_09535"
     CDS             complement(1515..1775)
                     /locus_tag="VN968_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29512.1"
                     /translation="MIKALAAIAIAAFVAAALTVLPGFAPQVEASVPVPLAKADRLDI
                     HAVGPNCSQKAWPNFEASCLRVAGKKTMAIKEARLVTADRTP"
     gene            2058..2555
                     /gene="coaD"
                     /locus_tag="VN968_09540"
     CDS             2058..2555
                     /gene="coaD"
                     /locus_tag="VN968_09540"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HXL29513.1"
                     /translation="MPRIALYPGSFDPVTNGHLDVVRHAVGLCDRLIVAIGVNSGKKP
                     MFSTDERLEMVAEVFAPIAAAAGCAFDCTTYDNLTVAAAQKHGATIMIRGLRDGTDLD
                     FEMQIAGMNETMAPDVHTVFVPASAAVRPITATLVRQIATMGGDVSAFVPPAVAARLK
                     AKFKG"
     gene            2607..3149
                     /locus_tag="VN968_09545"
     CDS             2607..3149
                     /locus_tag="VN968_09545"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXL29514.1"
                     /translation="MIRILAIFAALICAAPAIAQPLPANLDKPNAIVIDTTKGRVVFK
                     LRADLAPKHAERIKQLARDGYYNNVPFHRVIEGFMAQTGDGKNFNGTGGSKYPNLPAE
                     FSSVPFKRGIVGMARTSDPNSANSQFFIMFADGSSLNGQYTVIGEVVSGMDVVDKLKR
                     GEPVADPDKMVKVQVASDIK"
     gene            3186..3575
                     /locus_tag="VN968_09550"
     CDS             3186..3575
                     /locus_tag="VN968_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29515.1"
                     /translation="MAGAILFASTSLTAQAQPAQVNTLKEVFAKLGSCWKPPPASQAN
                     PGIDITVIVSFNRAGEILGHPKITYESEQATDNDRLAYRVAVMEALQRCTPMPFTEAM
                     AGAVAGRPFAVQFRNRKSPPKPIEKRA"
     gene            3572..4036
                     /locus_tag="VN968_09555"
     CDS             3572..4036
                     /locus_tag="VN968_09555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXL29516.1"
                     /translation="MTDTENTLILETTQGPVTIEMRPDLAPGHVARIKELVREGFYDG
                     IVFHRVIEGFMAQTGCPQGTGTGGSGKKLKAEFNREPHVRGTTSMARASSPDSGDSQF
                     FICFDDASFLNNQYTVWGKVTEGMENVDKIKRGEPVQNPDKIIKAHMAADAA"
     gene            complement(4171..4659)
                     /locus_tag="VN968_09560"
     CDS             complement(4171..4659)
                     /locus_tag="VN968_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HXL29517.1"
                     /translation="MKPIFAVCAAVAALGLMASSARAEISAEAARDSVAPFYKALNAE
                     LANESAELIKQSTAPQWVSCRGNDICNSRDEVIAGIGQRLKSVPDLKWEIKEILVSGN
                     QVTVRGEATGTPAGEFMGAPHTGKSFKVMSIDVHTLDGGKMVRSYHIEDWLGAVRQLS
                     AK"
     gene            4744..5670
                     /locus_tag="VN968_09565"
     CDS             4744..5670
                     /locus_tag="VN968_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL29518.1"
                     /translation="MNEIDLRRFDLNLMVVFEVLMAERNVTRAAERLGRTQSAVSHSL
                     SRLRSQLGDPLLIKAGVRMQPTAFAIDLMEQARPMLRGIERVLSPRRVFDPACSQRVF
                     RLAAPDFASTLFTSLLARLRSDAPGVSVEWTTAREPTLLDVAEGQVDVAIVPAQLRMP
                     DGVNSEAIGALKWRCFGRQGHPAFSKWGRKSWSHWPHLAVRVGDSLTSPVNLAAGAAR
                     LERRIAGWVPHFSAIAAILAGSDLLATLPSIAMAATLHPYRLDSREVPFPIAPLPHAM
                     IWSTGRSSHPEMTWLRDRLLPVVKSKFTDLAG"
     gene            5790..6866
                     /gene="queA"
                     /locus_tag="VN968_09570"
     CDS             5790..6866
                     /gene="queA"
                     /locus_tag="VN968_09570"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HXL29519.1"
                     /translation="MRTDLFDFELPTGHIALRPASPRDGARMLIVQPDGVLRDGIVAD
                     LPYWLEPGDQLVVNDTRVISAQLSGRRIGRETEPKIEATLIKRLDGSRWQALVRPAKK
                     LSAGDVVRFGHDGKICLLGHLDAEVEHKGDDGEVTLSFSFHGPALDSAIADLGRPPLP
                     PYIAGKRAPDERDAADYQTMFAANEGAVAAPTAGLHFTPALETALRQRGVGIHKVTLH
                     VGAGTFLPVKVEDTSAHKMHSEWGIISRDTSEALNTARAKGGRIVAVGTTSLRLLESA
                     TAPDGTIEPFTGETAIFITPGYGFRAVDILLTNFHLPRSTLFMLVAAFSGLDTMKRAY
                     AHAISGGYRFYSYGDACLLFRAHD"
     gene            complement(7027..7608)
                     /locus_tag="VN968_09575"
     CDS             complement(7027..7608)
                     /locus_tag="VN968_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29520.1"
                     /translation="MTRPARQKESRSLRLYARLLELYPPSFLQRHRAEMLQNFADLED
                     AEASKAKLWLLIGKDLAMSLMSHFFGSRLGHYVIAVLVTWILIFAIGYFFYGPTPGRP
                     ALHAFAGFLPGMLAMYFATRLYGTPQNSISHFFRSRFDIYVIGVFVAWILLFVIGYYI
                     YGPTRGHPALQVFGGFLPGMLAMYIATHVYGMP"
     gene            complement(7613..7936)
                     /locus_tag="VN968_09580"
     CDS             complement(7613..7936)
                     /locus_tag="VN968_09580"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXL29521.1"
                     /translation="MSKSRPQLPLTPAVFHILLALFGKERHGYDIMQQVRADSGDAVK
                     MGPGTLYGSLDRMIEAGLVAKGNTQDPRRIYYRLTALGQTTLRAETERLSRAALLARR
                     QLGSA"
     gene            complement(8124..9161)
                     /locus_tag="VN968_09585"
     CDS             complement(8124..9161)
                     /locus_tag="VN968_09585"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXL29522.1"
                     /translation="METQVKNDPVVAIAGVTGAVGSEFIATMDKRAFRVGKLKALASA
                     RSAGKTIDFRGEKITIEELDEASFEGVDIALFSAGASIARTFAPIAVKAGAVVVDNSS
                     AFRMDPNVPLVIPEINANRITEHKGIIAVPNCSAITALVPLWPIHRVNRIKRVILSTY
                     QAASGAGAAAMRELLDSTRACLHGQIHQPKVLPHFYAFNLFNHDSAVDPATGYNGEET
                     KVMQETRKIFEDDGIAVGVTCVRVPVLRAHSQAITFECENPITEHEVRAILIDAPGVK
                     IVDDRAKNYFPMPIDASGQDDVLVGRIRQDLSDASGHSISMFVAADQLLKGAALNAIQ
                     ITELLPQRVMP"
     gene            9406..10539
                     /gene="tgt"
                     /locus_tag="VN968_09590"
     CDS             9406..10539
                     /gene="tgt"
                     /locus_tag="VN968_09590"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HXL29523.1"
                     /translation="MSLPNHFDLLATDGPARTGRLTTPHGVVRTPAFMPIGTAGAMKG
                     MHWRDVRDAGTDIVLGNTYHLMLRPGAERIASLGGLQTFTGWQGPMLTDSGGFQVMSL
                     AQLRKVSESTVTFRSHIDGAKVELSPERAIEVQLLLGSDIAMQLDECVRLPAEHGDIE
                     RAMQLSLRWAERCTRAFETAAPGTMLFGIAQGGDVPELRRASARGLVDIGFHGYAIGG
                     LAVGEPQAVMLAMIEEVAPILPQDRPRYLMGVGTPEDMLEAVARGIDMFDCVMPTRNG
                     RHGVAFTRFGQVNLRNARHADDPRPLDEESLWPSTRSCARAYLHHLVKSGETLGAMLL
                     SEINIAYYQHLMRGIGEAIAKGTFESFRERTRADWAKGDIAPR"
     gene            complement(10644..10859)
                     /locus_tag="VN968_09595"
     CDS             complement(10644..10859)
                     /locus_tag="VN968_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29524.1"
                     /translation="MSATAAIRSTNTRHPDPRGCDLPTCPVCADSMVAAEASAYLSDN
                     VISYLWTCDTCGYGFVTKHSVKRFACN"
     gene            11082..12059
                     /gene="cysK"
                     /locus_tag="VN968_09600"
     CDS             11082..12059
                     /gene="cysK"
                     /locus_tag="VN968_09600"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HXL29525.1"
                     /translation="MNASPATSAVHRPGRGRVFDSIVDAACDTPIVRLRKLPQANGVH
                     ATILAKLEYFNPAASVKDRIGAAMIIAMEKAGLIHADTVLIEPTSGNTGIALAFVAAS
                     RGYRLKLVMPESMSIERRKMLAFLGAEIVLTPAAQGMKGSIATAEELVRTTPNAVMPQ
                     QFKNLANPEIHRRTTAEEIWNDTGGDIDVFVAGVGTGGTITGVGQVLKPRKPSLRIVA
                     VEPEESPVLSGGQHSPHKIQGIGAGFIPDILDRSVIDEIVTINSATAIETSRALARNE
                     GIPGGISSGAAIAAALQIGKRPESAGKTILAIVPSFAERYLSTALFEGI"
     gene            12062..12301
                     /locus_tag="VN968_09605"
     CDS             12062..12301
                     /locus_tag="VN968_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HXL29526.1"
                     /translation="MADQPRRPRTLNDARTEAEAAFKRTTTKTPEAPPKTTALPGVKE
                     LVSLRIDQDVLEHFQEGGPGWQDRINEALRKAAGK"
     gene            12317..12697
                     /locus_tag="VN968_09610"
     CDS             12317..12697
                     /locus_tag="VN968_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29527.1"
                     /translation="MHSRTGGFQVVLFVGLVAGLVSGLVLLMSGLLSTPASAESVPFN
                     CTILPGAEMASITMTNSLASDASCIVTCKFSTTKYDNDPQITCAKPVPAGKEVEMCRL
                     PDGGDRLVKFTEGHAECTRYQRRD"
     gene            complement(12749..14179)
                     /locus_tag="VN968_09615"
     CDS             complement(12749..14179)
                     /locus_tag="VN968_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXL29528.1"
                     /translation="MRYLAVIVCLICMIFTADAAKADRRVAFVVGNGAYKNVAQLPNP
                     PVDAKAMAAVLRNVGFEVVEGTNLTRDTMTERLLDFGKKAQGADVAVFFYAGHGIAIS
                     GTNYLLPVDADIKSEMDVKLGAAINIDLTLDQTMSDAKVKLVFLDACRDNPFAAKIKS
                     NSATRSVSVQSGLAEMKSGEGTLIAFATGPGQTALDGKEGTNSPFTRALIAHIATPGV
                     EIQQAMTEVRAQVNEETNKGQLPWGHTNLIGAVYLNPSAAPAASAAAPAVTGSTAVAS
                     TSSGSDVELEFWRSVKESNKPEELNAYLTNFPNGKFKSLALARIASLESGPSTTTRTL
                     TPGVDPATFTEQASQTTEDQIGLDKARRRDVQRRLTGLGFDTKVTGEFDDQTRAVITR
                     WQAARGYPKSGFFNKLQHKALLAEIVAGAPTAAREAPVEKPARPARASAGNAAAPPPP
                     QRGPDPGGAAFVGGLMGGVVGGMLRH"
     gene            complement(14366..15244)
                     /locus_tag="VN968_09620"
     CDS             complement(14366..15244)
                     /locus_tag="VN968_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXL29529.1"
                     /translation="MDRTPSKPRAALWMAGWLALMLIMTIAGRETTRELSVFQIMEVR
                     SILGFFMLYPLIRLHGGFSAMKTSRPLQHVGRNLIHYAAQLGWFFALTLIPLGQVVSI
                     EFTMPIWTAILAASFLGERITVWKLAAIVLGVVGVLVIVRPAAGEINPGQLIALGAAI
                     GFGISIAMMKSLTRTENTLTIIFWMLVIQSAAGFFPALYVWIWPSAYAFGWIVVIAFC
                     GTFCHYCMARAMLYADATVVLPMDFLRVPLTATAGWLIYSERLDMFTVLGAALILAGN
                     LLNLRAADPVPARIGT"
     gene            16062..16406
                     /locus_tag="VN968_09625"
     CDS             16062..16406
                     /locus_tag="VN968_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29530.1"
                     /translation="MTAHLHSSIWTLEVDDKPTLAFEAKKYSEANELCYEDWLRVELG
                     LQKFNGVPLCRADSALRIRLARPAEMVLYRRAVEANNSSDDSNLVYLIELDDVGRSVE
                     PTNPGAFPPQRT"
     gene            16776..17036
                     /locus_tag="VN968_09630"
     CDS             16776..17036
                     /locus_tag="VN968_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29531.1"
                     /translation="MPNSPQRLPSTTPKRPYFIGPEDAIALRELYEELPYAAMRAAEA
                     LRTDGTILRGDDLLGFSRAEDAVTEIIRRINEILENVHAKGP"
     gene            17204..17602
                     /locus_tag="VN968_09635"
     CDS             17204..17602
                     /locus_tag="VN968_09635"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXL29532.1"
                     /translation="MLVLQPAEWAKPRGFSHGVAVDGPGRWVVLAGQTGGDEKGDYQP
                     DMAGQVAVALRRIIKLLGEAGAGPEHIVRLTWYLTSRSEYEAAGAGIGAAWRETLGRN
                     FPPSTLLYIEGLVDIRAKVEIEVTAFVPTT"
     gene            17614..17829
                     /locus_tag="VN968_09640"
     CDS             17614..17829
                     /locus_tag="VN968_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29533.1"
                     /translation="MTNRSTSADFVTAFATGWPEEEPDVMILSLTTHKGVQDFALTPE
                     QALMIAKTMKQTAARLAVPKGVSAKRS"
     gene            complement(17859..18422)
                     /locus_tag="VN968_09645"
     CDS             complement(17859..18422)
                     /locus_tag="VN968_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29534.1"
                     /translation="MARRQACRAAADGKPRGNRPGRTITAFAGLRWLLLAAIALLAAS
                     GSGTASYAQSGPFTGMAGSWSGGGIVTLDDGSTERIRCRANYAVGAGGNGLNQNLLCA
                     SDSYKFDLRSNVMAEGGSLSGTWSESSRSINGTLEGRGGNGSFQVLASAAGFSANISL
                     TTHGNKQSVVIRSESQFRGASITLSRR"
     gene            complement(18367..18729)
                     /locus_tag="VN968_09650"
     CDS             complement(18367..18729)
                     /locus_tag="VN968_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29535.1"
                     /translation="MRHNRIRKIFCAFVVTSAAAALSAVPAAAQTPYDGLWNVTIVTK
                     AGSCEASTRSTLTVADGKVSAAGADVTGSIGREGLVRVSIGGAYANGQLSGNTGSGKW
                     NGASAGVPCSGRWEASRQ"
     gene            complement(18951..19541)
                     /locus_tag="VN968_09655"
     CDS             complement(18951..19541)
                     /locus_tag="VN968_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HXL29536.1"
                     /translation="MRITLVGSRHFGVTTFNMLRQHGVEIAGVVVADAEDRLAAAARA
                     ADIHVTVQADPRLVVAREIADRTDLIVTAHSHARIAREALAAAKLGGIGYHPSLLPRH
                     RGIAAVEWTIREGDPITGGTVYHLADRMDAGAIAAQEWVFVKKGETARELWERALAPL
                     GQKLLAEVIDYAKIHNALPAKAQDEQFATNAPRLPK"
     gene            19880..20158
                     /locus_tag="VN968_09660"
     CDS             19880..20158
                     /locus_tag="VN968_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29537.1"
                     /translation="MQSLNSQRQSNHGIHWPAIIATVLVQILVVLALSVAAAFYLEWS
                     SDVNQAEFASATHRSSLSDSNHLPEVSIPIRAVRGRAGCDPKAQRTGP"
     gene            complement(20178..20555)
                     /locus_tag="VN968_09665"
     CDS             complement(20178..20555)
                     /locus_tag="VN968_09665"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXL29538.1"
                     /translation="MVAMSLDRWHAGTARSRRGLPNRFRSAVVSMPRFTRLLNFLAQI
                     HGMVERRALPRQRIFKAGTIEFDGAGIDCIVRNLSAVGAGLEVASPAGIPHEITLNIR
                     SCEMRRHAYIVWRKEKRLGVAFG"
     gene            complement(20964..21563)
                     /locus_tag="VN968_09670"
     CDS             complement(20964..21563)
                     /locus_tag="VN968_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29539.1"
                     /translation="MSTSALLACLALLYGIANADPAVFIVTRADPDTAVSASVAAGLK
                     SVSVSLSGLGDRVGPQTPPGGTSHAIAGGAGGTTVATAEPFVADMQPFAEPFNMRGGP
                     AASQSTGLDYLIPVIDRAGTFTPNAADASTRALLTGVSFTFGAELYVVDATPVAEDPE
                     RSSGDMMILGFVGVGLMPYLVARALLPRPALSAFTIQSK"
     gene            complement(21842..22186)
                     /locus_tag="VN968_09675"
     CDS             complement(21842..22186)
                     /locus_tag="VN968_09675"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL29540.1"
                     /translation="MTNHQTFTDPGAGTWLTIDFLPGVELMPLAQPVPDGSIHRARLR
                     AGTTIPVHAHPADEFVLVLSGTIETAGRRCEAGTFWTTPAGTRQGPHVAVTEVELLTV
                     RLGAMGPFGDDG"
     gene            22250..22735
                     /locus_tag="VN968_09680"
     CDS             22250..22735
                     /locus_tag="VN968_09680"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HXL29541.1"
                     /translation="MKQGSRKSGAITLGAIEEGRRTNMRQLLLRASRIVNRHVVEGLH
                     ARGYADLRSTHTTLLSNIDLAGSTVTVAADRAGITKQAMGRLATELEGAGYIRVHGDP
                     KDARARVLQLTKTGKRLMLDSLEVMAELERRYARSVGRDRLAAVLRGLAAFVEGAAEW
                     D"
     gene            24105..24251
                     /locus_tag="VN968_09685"
     CDS             24105..24251
                     /locus_tag="VN968_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29542.1"
                     /translation="MTTIQAVVFGVMLALTPSMLVLAFLLWREGIGLADDGSNGEAYF
                     HRKY"
     gene            complement(24502..24762)
                     /locus_tag="VN968_09690"
     CDS             complement(24502..24762)
                     /locus_tag="VN968_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29543.1"
                     /translation="MTDELLAAMHGVLARYHRSIAKKATLDIVRQYHTDLAQRFADEA
                     AQIPRRTAILERLHEREVETRQTMAPSTNDVRDKPATRYDHP"
     gene            complement(24786..24995)
                     /locus_tag="VN968_09695"
     CDS             complement(24786..24995)
                     /locus_tag="VN968_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29544.1"
                     /translation="MTDKITYDIYRGTADRTLRLATMPGAGLPAHLKRKEWVLMPNGK
                     SPVHSDAARDVGMRGYCFFQVVESQ"
     gene            complement(25190..25444)
                     /locus_tag="VN968_09700"
     CDS             complement(25190..25444)
                     /locus_tag="VN968_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29545.1"
                     /translation="MGIFGRKVDDEFDALIHAIGRLLHGKGHDLQRAVLTDLVSRWIM
                     GHHPSQRRQALAIHVQAVRDLIELNEDALSDHKRGEPEDW"
     gene            complement(25755..26348)
                     /locus_tag="VN968_09705"
     CDS             complement(25755..26348)
                     /locus_tag="VN968_09705"
                     /inference="COORDINATES: protein motif:HMM:NF019501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
                     /protein_id="HXL29546.1"
                     /translation="MTPISVQGMEDSPERWLRDRMKCFLLALLVSVTGSAALAQTTGA
                     VNPSAASAQPSGNADVPPGGCMPIGLTTSGEIVFPIQCKEFIEQHRGKDVEQNSAAVE
                     PKPADVVEQKPAAVKEKPAAAVEDKPAVTQSEAMAPVAPENSQPVDKPFETVPLPKRA
                     ERKLREHVSLSGCQRYRTYDAASGTYRGYDGRVRPCR"
     gene            26802..27302
                     /locus_tag="VN968_09710"
     CDS             26802..27302
                     /locus_tag="VN968_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29547.1"
                     /translation="MSKTEARQHFGSVHIYWHGPAHCWDDTSTRRHHQIHQIHQVRHK
                     KNDPNWHDAMSEMPPDGGSASASTRLAPVPKQLAAAPTQLAAVPTQHAPAPTPWVNRW
                     VDIEQSPISSRWVDIAQVAPPLVIRAAEPLVTPRGVVMVIMFVIGSLAIIIFLQPSRR
                     NWRSTG"
     gene            complement(27359..27730)
                     /locus_tag="VN968_09715"
     CDS             complement(27359..27730)
                     /locus_tag="VN968_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29548.1"
                     /translation="MGNFIHQQNLAHYRRLLAEAELATSEDEIRHSMLMRLLAEEETN
                     GLLMPWTTTGRARGHGPSTEPAFNGTYVRRATDAATYPTQSPGDAGAEPSCASGSRDA
                     GQYGSSSKFMMVVATPPFRPS"
     gene            complement(27974..28288)
                     /locus_tag="VN968_09720"
     CDS             complement(27974..28288)
                     /locus_tag="VN968_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29549.1"
                     /translation="MRKFLLIAVVLLSATAAHAGLAVTAANTDVRAPEASLPSSAQRP
                     DASALAARRQGMAQQKMMMQKQMMMRKQMAMQREMQRHPIRTRLHFALLNFKRKLHYA
                     FH"
     gene            complement(29222..29347)
                     /locus_tag="VN968_09725"
     CDS             complement(29222..29347)
                     /locus_tag="VN968_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29550.1"
                     /translation="MLAEKFILVLETIISRTRPDGSSIVVSTSRHVPIKLPSKSG"
BASE COUNT         5770 a         8940 c         9270 g         5730 t
ORIGIN      
        1 tttttcgacc agcctgacgc cgctgcgcca ggtcagcatc aggaaagcga cgacggccgc
       61 cagcagcagc ggaaaccagc cgccctcaaa cagtttcgtc gagttcgccg cgaagaagat
      121 ggcgtcgatc acgaagaaga agccgttgac cgcgatcacg atgacgggcg aatagcccca
      181 ctggatcgcc accagagcgg ccagcaatgt cgtgatcgcc atcaacagcg acacggcaat
      241 tccgtaggcg cccgcaagcg cgtccgacgt gccgaagccg atcacggcgc cgagtgtcgc
      301 cgccgccagc aaccagttga ccagcggcac atagatctgg cccatcgcgt ggctggtggt
      361 gtggatgatg tgcatgcgcg gcaggaagcc gagctggatc gactgctgcg tcagtgaaaa
      421 caccccggag atgatggcct gtgaggcaat cacggtggcc acggtggcaa aggcgacgag
      481 cggatagtgt agccagtcag gtgcgagctg atagaaggga tggtcgagcg cgctggcgtc
      541 ggtgatcagc agtgccgcct gcccgaagta attgaggacg agggctggga gtgcgacgat
      601 gaaccatgcg agccggatcg gtacccggcc gaaatggccc atatcggcat acatcgcctc
      661 gcctccggtc acggcgagaa aggcggcgcc aaggaccgcg aagctgatgt ggaagtcctc
      721 gtgtatcaat aaatcgaaag cataaagcgg acttagcgcg gcaagcacgc cgggcgcccg
      781 cacgatgccg tggactccga gcagtgcaat caccacgaac caggcgagca tgaccggccc
      841 gaagatattg ccgataaaac ctgcgccctt tttctgaacg atgaaaagac cgatcaggat
      901 ggcgatggtc agcggcacca ccgcgggcgc cagcgaaggc gcatcgacct tcaggccctc
      961 gattgcgctc agcaccgaga tcgccggcgt gatggcgccg tcgccgtaga gcagcgccgc
     1021 gccgacaagg ccgacgacca ggagctgcgc acgccacgtc cccggccggg cattgcgcgc
     1081 ggaaagcagc gccagcagcg cgacgatgcc gccttcaccg cggttgtcgg cgcgcaggat
     1141 cagcagcgca tatttcagcg aaattatcag gatcagcgcc cacaggatca gcgataccac
     1201 gccgaggatc gcttcagggg caagcggacc gccgtgggcg gccgctttgg cggcttcctt
     1261 cagcgcgtag aggggactgg taccgatatc gccatagaca acgccaagcg cgccgattgt
     1321 tgccgcccaa ggaagcgcgc cggaaccggg tggaatggcg gcgggaacgg cagtcactgg
     1381 cggagccccc aagcggtttc aagggtatcc aaggatgtcg cgcccgtcgg ctggaggggc
     1441 gcgatcgcat gccagtctgc gacggggctt cgcaaattac tatgagattt gtcgggttgc
     1501 ggaaagcagt tgcgttatgg cgtgcggtcg gcagttacca gccgggcttc cttgatggcc
     1561 atggtcttct tgccggcaac gcgcaggcag gatgcctcga agttcggcca ggccttctgc
     1621 gaacagttcg ggcccacggc gtggatatcc agccggtcgg ctttggccaa cggcacgggc
     1681 acgctggctt cgacctgcgg ggcaaatccg ggaagaacgg taagtgcggc agcgacaaac
     1741 gcagcgatgg cgattgctgc aagagccttg atcatgacgg tcccctgtca ctgggccttt
     1801 acggcccgtc gtttcgttaa ccatttgata gccagcaccg gtttccggat gtcttcgccg
     1861 attgccagaa tggtttcgtc ccgcctgagt tttgtttcgt cgcccgttcc ggacgaaaca
     1921 aagccggctt gcatagaaga cgcgccgacc ccgcaagtca ttcagacatc tgtgaaatat
     1981 aaaaacccgg gtattagctt cggggcgggg aaccagttcg gcttgccggg ccgctccggg
     2041 cgcggtagag ggggcccatg ccccgtatcg cgctttatcc cggttccttc gatcccgtca
     2101 ccaatggcca cctcgatgtg gtccggcacg ctgtcggcct gtgtgaccgg ctgatcgtcg
     2161 ccattggggt caactccggt aaaaagccga tgttctcgac cgacgagagg ttggaaatgg
     2221 tcgcggaggt ttttgccccc atcgctgccg ccgccggctg cgcgttcgac tgcacgacct
     2281 acgacaacct cacggttgcc gccgcgcaaa agcatggcgc gaccatcatg atccgtggcc
     2341 tgcgcgacgg caccgacctc gatttcgaga tgcagatcgc cggcatgaac gaaaccatgg
     2401 cgcctgacgt gcataccgtg ttcgttccgg cttcggcggc cgtccgcccg atcaccgcca
     2461 cactggtgcg ccaaatcgca accatgggcg gcgacgtctc ggcgttcgtg ccgccagcgg
     2521 tcgcggcccg cctcaaggcc aaattcaagg gctagctgac aggctcgccg gcactctctg
     2581 acctcatgtt ctgacttgga gttttcatga tccgaattct agctattttt gctgcgctga
     2641 tttgcgcggc ccccgcgatc gcgcagccgc tgcctgccaa tctcgacaag ccgaatgcga
     2701 tcgtcatcga caccaccaag ggccgcgtcg tcttcaagct gcgcgccgac ctcgcgccga
     2761 agcacgccga gcggatcaag caactggcgc gggacggcta ctacaacaac gtgccgttcc
     2821 atcgcgtcat cgaaggtttc atggcgcaga ccggcgacgg caagaatttc aacggaaccg
     2881 gcggctcgaa atatccaaat ctgcctgctg aattctccag cgtgccgttc aagcgcggta
     2941 tcgtcggcat ggcgcgtacc agcgatccca actcggcgaa ttcgcagttc ttcatcatgt
     3001 tcgccgacgg ttcgtcactg aacggccagt acacggtgat cggcgaggtg gtgtcgggca
     3061 tggacgttgt cgacaagctc aagcgcggtg agccggttgc cgatcccgac aagatggtga
     3121 aggtgcaggt cgcatccgac atcaagtgag cggcggtggc ctggcctcgc caaccgcgga
     3181 catggctggc aggggcgata ctgttcgcct caacgagttt gacggcgcag gcccagccgg
     3241 cacaggtcaa taccctcaag gaggtgtttg cgaagctcgg gagctgctgg aagccgccgc
     3301 cggcgtcaca agccaatccc ggaatcgaca tcacggtgat cgtcagtttc aaccgcgccg
     3361 gcgagatcct cggccatccg aagatcacct atgaatcgga acaggccacc gacaatgacc
     3421 ggctggcgta ccgcgtcgcc gtgatggagg cattgcaacg ctgcacgccg atgccattta
     3481 ccgaagcgat ggccggcgcc gtggccggcc ggccgttcgc ggtccagttt cgcaatcgga
     3541 aatctccacc caaacccata gagaagagag catgactgac accgaaaaca ctttgatcct
     3601 tgaaaccacc caggggccgg tcacaatcga aatgcggccc gatctcgcac ccggtcacgt
     3661 cgcccggatc aaggaactgg tacgcgaggg attctacgac ggtatcgtgt tccaccgcgt
     3721 gatcgagggg ttcatggccc agaccggatg tccgcaggga accggcaccg gcggctccgg
     3781 caagaagctc aaggccgagt tcaacaggga accgcatgtg cgcggcacca cctcgatggc
     3841 gcgcgcctcc agcccggatt ccggcgacag ccagttcttc atctgcttcg acgatgcctc
     3901 gttcctcaac aaccagtaca cggtctgggg caaggtgacc gagggcatgg aaaacgtcga
     3961 caagatcaag cgcggcgagc cggtacaaaa ccccgacaag atcatcaagg cgcatatggc
     4021 cgcggacgcg gcgtgaagcc caaatcgtca tacgcgggca aaagcgcgaa gcgcgtcttc
     4081 gccggatgac ccgcgtatcc atcaatcttc agaagagtct aacgtagaag atggattgcc
     4141 ggatcaagtc cggcaatgac ggtctgcggc tcacttcgcc gagagctgac ggaccgcacc
     4201 cagccaatcc tcgatgtggt aggaacgaac catcttgccg ccgtcaaggg tgtgaacgtc
     4261 gatcgacatc accttgaatg acttgccggt atgcggcgcg cccatgaatt cgcccgccgg
     4321 tgtgccggtt gcttcgccgc gcacggtaac ctggttgccc gagaccagga tctccttgat
     4381 ttcccatttc aggtcgggga ccgatttgag acgttgtccg atcccggcga tgacttcatc
     4441 gcggctgttg cagatatcgt tgccgcggca ggagacccac tgcggcgcgg tcgattgctt
     4501 gatgagttcg gcgctctcgt tggcgagttc ggcattcaag gctttgtaga acggcgcgac
     4561 gctgtcgcgt gccgcctcag cgctgatttc ggcccgggcc gaagatgcca tcaagcctag
     4621 ggcggcaaca gcggcgcaga cggcgaagat tggtttcatg gcggctccag tgacttgatt
     4681 gagggggcgc cggtggtatg aaccttattg gataatcgat ccaatgagta atcatggcac
     4741 atcatgaatg aaatcgattt acgtcgcttc gacctcaacc tcatggtggt gtttgaagtc
     4801 ttgatggccg aacgtaacgt cacccgggcg gccgagcggt tgggacggac gcaatcggcc
     4861 gtcagtcatt cgctgtcgcg attgcgcagc caattgggcg atccgctgct gatcaaggcc
     4921 ggcgtaagga tgcagccgac ggcgttcgcc atcgatctca tggagcaggc ccggccaatg
     4981 ctgcggggca tcgagcgcgt gctttcgccg cgacgtgttt tcgatccggc atgttcgcag
     5041 cgggttttcc ggttggcagc ccccgatttc gcatcgaccc tgttcacaag cctgcttgcg
     5101 cgactgcggt cggacgcgcc cggtgtttcc gtcgagtgga cgacggcgcg ggagcccacg
     5161 ctgctcgacg tggcggaggg ccaggtcgac gtcgcgattg tgccggcgca acttcgcatg
     5221 cctgacggcg tcaacagcga agccatcggg gcgctgaaat ggcgatgctt cggccgtcag
     5281 ggccacccgg cgttttccaa atgggggcgc aagagctggt cgcactggcc gcatcttgcc
     5341 gtacgtgttg gcgacagcct gacgagcccc gtgaatctcg cggcgggcgc ggccaggctg
     5401 gagcgaagga tcgccggctg ggtgccgcat ttctccgcca tcgctgccat tctggccggt
     5461 tcggatttgc tggccaccct gccatcgatt gcgatggccg cgacgttgca tccttaccgg
     5521 ctggacagca gggaggttcc atttccgatc gcgccgctcc cgcacgcgat gatctggagc
     5581 accggccgaa gcagccatcc cgagatgacc tggctgcgcg atcggttgct gcctgtcgtc
     5641 aaaagcaaat ttaccgatct cgctggttaa atcacgcgcg agaccccctc cgttttgacg
     5701 gtcgaaatgg ccgtgcggcg tgttacaccg gcgacccgtt atgcgacgcg ctcaggtgct
     5761 tcgcgaagat gatcaagtcc cggaccagca tgcgcaccga cctgttcgac ttcgagcttc
     5821 ccaccggcca catcgcgctg cgcccggcga gcccgcgcga tggcgcgcgg atgctgatcg
     5881 tgcaacccga cggtgttttg cgcgacggca tcgtggccga tcttccgtac tggcttgagc
     5941 ccggcgatca gctggtggtg aacgatacca gggtgatatc ggcccagctg agtggccgcc
     6001 gcattggccg ggaaaccgag ccgaagatcg aggcgaccct gatcaagcgc ctcgatggct
     6061 cgcgctggca ggcgctggtc aggcctgcca agaaactgtc ggcaggcgat gtggtccgct
     6121 tcggccatga cggcaagata tgcctgctcg gtcatctgga cgccgaggtc gagcacaagg
     6181 gtgacgacgg cgaggtgacg ctgtcgtttt catttcacgg ccccgcgctc gattccgcca
     6241 tcgccgatct gggccggccg ccgctgccgc cttacatcgc cggcaagcgc gcgcccgacg
     6301 aacgcgatgc cgccgactac cagaccatgt tcgcggccaa cgaaggcgcg gtcgcagccc
     6361 cgactgcggg actgcatttt acccccgcgc tggagacggc actgcgccaa cggggcgtcg
     6421 ggattcacaa ggtaacattg catgtcgggg cagggacctt tttgccggtc aaggtggaag
     6481 acaccagcgc tcacaaaatg cactccgaat ggggcatcat ttcccgcgac acatcagaag
     6541 cgctgaacac agcaagggcc aaaggcggcc gcattgtcgc cgtcggcacg acctcgctgc
     6601 ggctattgga aagcgccacc gcgccagatg gcacgatcga gccgttcacc ggggaaaccg
     6661 cgatcttcat cacgccgggt tatggttttc gcgccgtcga tatcctcctg accaattttc
     6721 acctgccgcg ctcgacgctg ttcatgctgg tggcggcctt cagcggcctc gacaccatga
     6781 agcgggccta tgcccatgcg atttccggcg gctaccggtt ctattcctat ggcgatgctt
     6841 gtctgctgtt tcgtgcccac gactagggcg cggactcgtt agagtgtagc cacattcgga
     6901 ttgacaggcc gacggacctt gaaaatgtct gctcatcggg caagaccgca agtgaacggc
     6961 acgcggtcag aacgacacga gtgaccctca gccgacctaa ctcatgcagc cgggatgcga
     7021 gctgttttac ggcattccat agacatgtgt cgcgatgtac atggccagca tcccggggag
     7081 aaagcctccg aaaacttgca aagccggatg accacgcgtc gggccgtaga tgtaataccc
     7141 gataacgaac agcaatatcc aagcgacaaa gacgccgatc acgtatatgt caaaccgtga
     7201 cctgaaaaag tgcgaaatgc tgttttgcgg cgtcccatag agacgtgtcg cgaagtacat
     7261 ggccagcatc ccggggagaa agcctgcgaa agcatgcaaa gccggacggc cgggcgtcgg
     7321 gccgtagaag aaatacccga tcgcgaagat caatatccac gtaacaagga cagcgatcac
     7381 gtaatggcca agccgtgacc cgaaaaagtg cgacatcagg ctcattgcga ggtcctttcc
     7441 tatcagcagc caaagtttcg ccttcgacgc ctccgcgtct tccaggtcgg cgaagttttg
     7501 cagcatctcc gcgcgatgcc gttgcagaaa tgacggcggg tagagttcca acagccgcgc
     7561 gtagaggcgt aacgaccggg actccttctg ccgcgcgggg cgcgtcatgg cgtcacgcgc
     7621 tcccgagttg gcggcgcgcg aggagggccg cgcgagacag ccgctccgtt tccgcgcgca
     7681 acgtggtttg gccaagcgcc gtgagcctgt agtaaatgcg gcgcgggtct tgggtgttgc
     7741 ctttggcaac caggcccgcc tcgatcatcc ggtcgagcga gccgtacagc gttcccggac
     7801 ccatcttcac cgcgtctccc gagtccgccc tgacctgctg catgatgtcg tagccgtggc
     7861 gctccttgcc gaacaaggcg agcaggatgt ggaagacggc gggtgttagc ggcagttgag
     7921 gtcgcgactt gctcatgcgg ctatgatata tccgtgacgg atatagtcaa tagcaacggc
     7981 gaacttgcac cattaatacc ctaacgtcgc catggcactt atcggacctc ctgcgatgtc
     8041 cgactggagt ctgcaatggg caacaaggcg gacgtccgtc agcgctatgg atttatgagt
     8101 tcacgcccta acagggcgtg ggctcagggc atcacccgct gcggcagcaa ttccgtgatc
     8161 tgaatcgcgt tgagcgcggc tcccttcagc aattggtcgg ccgccacaaa catcgagatc
     8221 gaatggccgc tagcgtcgct gagatcttgg cgaatgcggc cgaccaggac gtcatcctgg
     8281 cctgacgcat cgatcggcat cggaaagtaa ttttttgccc gatcatcgac gatcttcaca
     8341 cccggcgcat cgatgaggat cgcgcgcacc tcgtgctcgg tgatcgggtt ttcgcattcg
     8401 aaggtgatgg cctggctgtg cgcgcgcagc accggcacgc gcacacaggt cacgccgacc
     8461 gcaatcccgt catcttcgaa gatcttccgg gtctcctgca tgactttggt ttcctcgccg
     8521 ttgtagccgg ttgccggatc gaccgcgctg tcgtggttga acaggttgaa ggcgtaaaag
     8581 tgcggcagca cctttggttg atggatttgc ccgtgcaggc acgcacgcgt tgaatccagc
     8641 aactcccgca tcgccgccgc acccgcgcca ctggccgcct gataggtcga caggatcacg
     8701 cgcttgatgc ggttgaccct gtgaatcggc cataggggca ccagcgcggt aatcgccgag
     8761 cagttcggaa cagcgataat gcctttgtgc tcggtgatgc ggttggcatt gatttcgggg
     8821 atcaccagcg gcacgttcgg atccatccgg aaggccgacg aattgtcgac cacgacggcg
     8881 ccggctttca ccgcgatcgg cgcgaacgtc ctggcaatgc tcgcacccgc cgagaacagc
     8941 gcgatatcga cgccttcgaa tgaggcctcg tcgagctctt cgatcgtgat cttctcgcca
     9001 cggaagtcga tcgtcttgcc cgccgagcgc gcgctcgcca gcgctttgag cttaccgacg
     9061 cgaaaggcgc gcttgtccat cgtggcgatg aattcggaac cgacggcgcc ggtcacgccg
     9121 gcaatggcga caacgggatc gttcttcact tgggtctcca tcaggcaata aaaaagcccc
     9181 ggaccttttc aggcggggct ccggtccaga tgatgcgatc gtccaactac gcgcgcacgt
     9241 ctcccgaagc ccgacagggc tttgcggttt tggtggtgcg tttggtgcgg ttcatcatgg
     9301 cgcgggaaca tatggggggc tttgaccagc gtcaacggct ttcgggcaga aaatcggctt
     9361 caacgaggtt gaacaggatg ccgttgtgac gcaataagct ggtgcatgag ccttcccaat
     9421 cacttcgatc tcctcgcgac cgacgggccg gcgcgcaccg gccggctaac gacaccgcac
     9481 ggcgtggtgc gcacgccggc cttcatgccg atcggcaccg ccggcgcgat gaagggcatg
     9541 cattggcgcg atgtccgcga tgccggcacc gatatcgtgc tcggcaatac ctatcatttg
     9601 atgctgcggc cgggcgccga gcgcattgcc tcgcttgggg gcttgcagac ctttaccggc
     9661 tggcagggcc cgatgctgac cgattccggc ggcttccagg tgatgtcgct ggcgcagtta
     9721 cgcaaggtga gcgaaagcac ggtgaccttt cgctcgcaca tcgatggcgc caaggtcgag
     9781 ctgtcgcctg agcgcgcgat cgaggtgcag cttctgctcg gctccgatat cgccatgcag
     9841 ctcgacgaat gcgtgcggct gccggcggag cacggcgata tcgagcgggc gatgcagctc
     9901 tcgctgcgct gggcggagcg ctgcacgcgc gcgttcgaga ccgcggcccc cggaaccatg
     9961 ctgttcggga tcgcgcaggg tggcgacgtg ccggaactgc gccgggcaag cgcgcggggc
    10021 ctggtcgaca tcggctttca cggctacgcc atcggaggcc tcgcggtcgg cgaaccgcaa
    10081 gcggtgatgc tggcgatgat cgaggaggtg gcgccgattt tgccgcagga tcgcccgaga
    10141 tatctgatgg gcgtcggtac gccggaagac atgcttgagg cggtggcgcg cggcatcgat
    10201 atgttcgatt gcgtcatgcc gacgcgcaat gggcgccatg gcgtggcgtt cacgcggttc
    10261 ggtcaggtca atttgcgcaa cgcccgccac gccgacgatc cgcggccgct tgacgaggaa
    10321 agcttgtggc catcgacgcg cagttgcgcg cgggcgtacc tgcatcatct cgtcaagtcg
    10381 ggtgagacgc tcggggccat gctgctgtcg gaaatcaata tcgcgtatta ccagcacctg
    10441 atgcgcggca tcggtgaggc tattgcgaag gggacgttcg aaagctttcg ggaacgcacg
    10501 cgcgccgact gggcgaaggg cgatattgcg ccgcgctaga gccctttttc gtttcaatag
    10561 aatcgaaacc ggggctctag atttttgttt atcggatttt tgtttgacgc gtcttcttca
    10621 cgcgaaccgg tgccgtcgca agctcagttg caggcaaatc gcttgaccga atgcttggtt
    10681 acgaaaccgt aaccgcaggt atcgcaggtc cagagatagc tgatgacgtt gtcggacaga
    10741 taagccgacg cttccgcagc caccatggaa tccgcgcaaa ccgggcaggt cggcagatcg
    10801 catccgcgcg ggtcgggatg acgcgtattg gtcgaccgga ttgcagcagt agctgacatc
    10861 gtgacctccg tggcgatctg aactcgagct ttgtcatact ctgatttgtt tgacgatgtg
    10921 gcaacacaat tgcgttggtt ttacgtttat ttggttgcta ttaaattttg caacgcagcg
    10981 aggacccatc cgcgggattc tggacttgca cggcggcacc agctgtccct aatggagaat
    11041 agcctctaaa tgcacagaca tcgcgtgaca ggagtttcgt tatgaatgct tcgccagcta
    11101 cgagtgcggt gcaccgtccc ggccgcggca gggttttcga cagcattgtc gatgcggcct
    11161 gcgacacgcc gatcgtccgt ctgcgcaaat tgccgcaggc gaacggggtg cacgcgacca
    11221 ttctcgccaa gctggaatat ttcaatccgg ccgcgagcgt gaaggaccgc atcggggctg
    11281 caatgatcat cgcgatggag aaggctggcc tcatccacgc cgatacggtg ctgatcgagc
    11341 cgacctcggg gaataccggc atcgcgctgg cattcgtcgc cgcctcgcgc ggctatcggc
    11401 tcaagctcgt gatgccggag tcgatgtcga ttgagcggcg caagatgctg gcgtttctcg
    11461 gtgccgaaat cgttctgaca cccgcggccc agggcatgaa gggctcgatc gcgaccgcgg
    11521 aagagctggt gcgcacgacg cccaacgccg tgatgccgca gcagttcaag aacctcgcca
    11581 atccggaaat tcaccgccgc accacggcgg aggaaatctg gaacgacacc ggcggcgaca
    11641 tcgatgtctt cgtggccggg gtcggtaccg gcggcaccat caccggcgtc gggcaggtgc
    11701 tcaagccacg caagccgtcg ctgcggatcg tcgcggtgga gcccgaggaa agtccggtac
    11761 tctccggcgg ccagcattca ccgcacaaga tccagggcat cggcgccggc ttcattccgg
    11821 acatcctgga ccgttcggtg atcgacgaga tcgtcacgat caacagcgcg accgcgatcg
    11881 aaacctcgcg cgcgctggcg cgcaacgagg gcattcccgg cggcatttcc tcaggtgccg
    11941 cgatcgccgc agcccttcaa atcggcaaac gtccggaaag cgccggcaag accatccttg
    12001 cgatcgtgcc gtcgttcgcc gagcgttatc tgtcgacggc gttgttcgaa ggaatctaga
    12061 cgtggcggat caaccaagac gaccgagaac cctgaacgac gcgcgcaccg aagccgaagc
    12121 ggcgttcaag cggacgacga ccaagacgcc ggaggcgccg cccaaaacca ctgcccttcc
    12181 gggtgtgaaa gagctggtct cgctgcggat cgatcaggat gtgctcgagc actttcagga
    12241 gggcgggcca ggctggcagg atcggatcaa tgaggcctta agaaaagcag ctggcaaata
    12301 gtcgcgggcg gtatcgatgc attcgcgcac cggcggcttc caggtcgttc tattcgtcgg
    12361 ccttgtcgcc ggtcttgttt ccggccttgt gctgctgatg tccggccttc tgtcgacgcc
    12421 ggcgtcggcg gagtcggtgc cgttcaactg cacgattttg ccgggagcgg agatggccag
    12481 catcacgatg accaattcgc tggccagcga cgcgtcctgc atcgtgacct gcaaattctc
    12541 gacgaccaaa tacgataacg atccgcagat cacttgcgcc aaaccggtcc cggccggcaa
    12601 ggaagtcgag atgtgccggc tgcccgacgg tggcgacagg ctggtgaaat tcaccgaggg
    12661 ccacgccgag tgcaccagat accagaggcg ggattgaaaa aagcccggga tgagccgggc
    12721 tttttgacga tccagggcct tttgatgatc agtgccgaag catgccgccc accacaccac
    12781 ccatcaggcc gccgacaaag gcagcacccc cgggatccgg accacgctga gggggagggg
    12841 gagcagcggc attgccggcg ctggcacgtg caggacgggc cggcttttct accggcgcct
    12901 cgcgggcggc ggttggggcg cccgcgacga tttccgccaa cagcgccttg tgctgcagct
    12961 tgttgaagaa gccgcttttg ggatagccgc gcgcggcctg ccagcgcgtg atcacggcgc
    13021 gcgtctggtc gtcgaactcg ccggtgacct tggtatcgaa accaaggccg gtcagccggc
    13081 gctgtacgtc gcgccgacgg gccttgtcga gaccgatctg gtcttcggtg gtttggcttg
    13141 cctgctcggt gaatgttgcg gggtcgacgc ctggggtcag ggtacgggtc gtggtcgacg
    13201 gaccgctttc cagagaggcg atccgggcca gcgccagcga cttgaactta ccgttcggga
    13261 agttggtcag gtaagcattg agttcttccg gcttgttcga ttctttcacc gagcgccaaa
    13321 attccagttc gacgtcggaa ccgctggacg tggaggcaac ggcagtgctg ccggtaacgg
    13381 ccggcgcagc cgcgctcgcc gccggcgccg cgctcggatt gagatagacc gcgccgatga
    13441 ggttggtgtg gccccagggc aattgaccct tgttggtctc ttcgttgacc tgggcgcgga
    13501 cctcggtcat cgcctgctgg atctcgacac ccggcgtcgc gatgtgggcg atcagggcgc
    13561 gggtaaacgg actgttggtg ccttccttgc catccagcgc ggtctggccg gggccggtcg
    13621 cgaaggcgat cagggtgcct tcgccggatt tcatctcggc aaggccggac tgcaccgaca
    13681 cgctgcgggt cgcggaattc gacttgatct tggcggcaaa gggattgtca cggcaggcgt
    13741 cgaggaacac cagcttgacc ttggcatcgc tcatggtctg gtcgagcgtg aggtcgatat
    13801 tgatcgcggc gccgagcttg acgtccattt ccgatttgat atcggcgtcg accggcaaca
    13861 aatagttggt gccgctgatg gcgatgccgt ggccggcata gaaaaatacc gcgacgtcgg
    13921 cgccttgcgc cttcttgccg aagtcgagca gccgttcggt catggtatcg cgcgtcaggt
    13981 tggtgccttc gacgacttcg aagccgacat tgcgaagtac cgccgccatc gccttggcgt
    14041 cgaccggagg gttgggaagc tgggcgacat tcttgtaggc gccgttaccg acgacaaagg
    14101 cgacgcgccg gtcggccttg gcggcgtccg cggtgaagat catgcaaatc aggcaaacga
    14161 tgacggcgag gtaacgcatc tgaaatcccc aggctgagtc gagagcaatt tcggcgggca
    14221 acgcatcggc gggaacctac gaaaataccg cagttcgcgc cacgaaaatt tgagctgttc
    14281 ccccaatctt ggcagccgcc atcgccgcgc gtattgcgtg acctagatca cgattcgcgg
    14341 ccccgacccg gagatcgcgg accgatcagg tgccgatccg cgccgggacc ggatcggccg
    14401 ctctcagatt caagagattg ccggcaagga tcagcgccgc gccgagcacc gtgaacatgt
    14461 ccagccgctc cgaatagatc agccagccgg cggtggccgt cagcggaacc cgcaggaaat
    14521 ccatcggcag caccacggtc gcgtccgcat acaacatcgc gcgcgccatg cagtagtggc
    14581 agaaggtgcc gcagaacgcg atcacgacga tccagccaaa ggcataggcc gaaggccaga
    14641 tccagacgta gagcgcggga aaaaatccgg ccgccgactg gatcaccagc atccagaaaa
    14701 tgatggtcag ggtattttcg gtgcgggtca gggacttcat catggcgatg gaaatgccga
    14761 atccgatggc ggcacccagt gcgatcagtt gaccgggatt gatttcgccg gcggccggac
    14821 gaacgatgac gaggaccccg accacaccga gcacgatggc tgcaagcttc cagaccgtga
    14881 tccgctcacc aagaaaactc gcggcaagga tcgcggtcca gatcggcatg gtgaattcga
    14941 tcgataccac ctggccgagc gggatcagcg tcagcgcgaa gaaccagccg agctgtgcgg
    15001 cgtagtgaat gagattgcgc ccgacatgct gcagcggccg cgatgtcttc atcgccgaga
    15061 atcctccgtg gaggcggatc agcggataca gcatgaagaa gccgaggatc gagcgcacct
    15121 ccatgatctg aaacacgctc agttcgcgcg tcgtctcgcg gccggcgatc gtcatgatca
    15181 gcatcagggc aagccagcct gccatccaca gcgcggccct gggtttggaa ggtgttcggt
    15241 ccatcggggg gattgccagg caggccgcgc aagcgccggc gcggctgggt gggggagggc
    15301 aggccggtat cggcgacctc atgcggactt gcaacacgca aatatgctgc gctgcgcgca
    15361 cttaatttcg gtgtgggagc gcccgcccga tcggtgctaa gggatcggct gatcttgtcc
    15421 gtcattcccg ggcgcgtcca acgggtccgc gcaagcgcgg cccgatgaca gattccgacg
    15481 cgaacccgga atctccaacc aacctacccc ccctcaggag cgccaacagt tcaaaggatt
    15541 ggcgaagctg atagcgtgaa taatcggaac ccgccagcgc acattgcaca cgtacagggc
    15601 gagaatcccc gtggcaatct tctcagaccc ctacttcgtc aggggcctcg gcaaaaccgc
    15661 cgtaaacggt acgacggcga gcgctcggcc ctcctcatct gcgattacga aagcttccgg
    15721 aacttctggc ctaccactct tgatggcctc agccaaaagt tcacgcgccg ctagtatcgc
    15781 ctcgtgctca gctgcagaaa ggtccggcaa gtctacgcct tcgtcatcgg gaactatttg
    15841 gtcgtccgct tgtagatgga aataaaatcg tcccatggcg acctcccatt catgagggca
    15901 acctaatcga tggtcgcttg ttcctccggt cctttccttg cgccgcagca ttacaactaa
    15961 attcgattct tgttgggaac caccggcttc aggttcggtt tctcttagtc cacagactcc
    16021 ggcccgttaa gttcgatttt ctgagtgcag gggtggttca gatgaccgcg cacctccatt
    16081 cttcgatttg gacgctcgag gtcgacgaca aacccacgct ggcttttgaa gccaagaagt
    16141 acagcgaagc gaatgagctt tgctatgagg attggctgcg cgttgaactg ggcctgcaaa
    16201 aattcaacgg cgtcccgttg tgtcgtgccg actcagccct aaggatacgg ctcgcccggc
    16261 cggctgaaat ggtcctgtat cggcgagcgg tcgaagctaa caattcgtca gacgacagca
    16321 atctggtcta tttgatcgaa ctggacgacg tcggtcgttc cgttgaaccg accaaccccg
    16381 gggcatttcc gcctcagcgt acctaattgc cgcacgacgc tacccctact aatgcgttga
    16441 tcggctcctg caaagctcaa atggaaagag catccaaagg agacggcact cgcgttgtag
    16501 gaataccgtt ttcattacgc cccagttttt cgcctgacga gattttcact attcgcgggg
    16561 gagaagagat atttatagat ttattggccg ttcctctcga cccgagggcg actcccgttc
    16621 ttgccaaaat tgcagaaaga acgttgcgga aaacgccccg cagacggcgg gcttgatgtt
    16681 ggcatggtcc ggtttgaatt tagtggctct tgacggacgg tcgccccgtc ccggctcctg
    16741 tgctatcctt ggcggaaagc aggagggata tcgcaatgcc caattctcct caaaggctgc
    16801 cttccacaac tccgaagcgt ccgtatttta ttggaccaga ggatgcgatt gcgttgcgag
    16861 aactttatga agagctgccc tacgccgcaa tgcgtgcggc ggaagcgctg cgcacggatg
    16921 ggaccattct gagaggagat gacttgctgg gtttctcgcg tgcggaagat gccgtcacgg
    16981 aaatcatccg acgaattaac gagattcttg agaacgttca tgccaagggg ccctaaaggc
    17041 gagaagcgca gcaatgcaag cacaaagctg gggcgtcgtc cggggcgatc ggtgccaagg
    17101 gatcgctgat ctcttccgtc attccgggtt cgcgtcatcc aggtcggctg tcgctgacgc
    17161 gccccggaat gacgatacct gacaaacgct ggagaaccag ttcatgcttg tcctgcaacc
    17221 ggccgaatgg gcaaagccac gcggcttttc gcacggtgtc gcggtcgacg gtcccggccg
    17281 atgggtcgtc cttgccggcc agaccggcgg cgacgagaag ggcgactatc agcccgatat
    17341 ggccggacag gtcgcggtgg cgttacgaag gatcatcaag ctgctcggtg aagccggggc
    17401 cgggcccgag cacatcgttc gcctgacctg gtatctgacc agccgcagcg aatatgaggc
    17461 cgccggcgcc ggcatcggtg cggcctggcg cgagacgctg ggacgaaact tcccgccctc
    17521 gacgctgctc tacatcgaag gtctcgtcga tatcagagcc aaggtcgaga tcgaagttac
    17581 cgcgttcgta ccaacgacat aggaagtgta gagatgacca accgatccac atccgcagat
    17641 tttgtgacgg ccttcgccac cggctggccc gaggaggaac cggatgtcat gattctttcg
    17701 ctgaccacgc ataaaggcgt tcaggatttt gcgctcaccc cggaacaggc gctgatgatc
    17761 gcaaagacca tgaaacagac agccgcccgg ctggctgtgc caaaaggcgt tagcgccaaa
    17821 aggagttagc cgacgcggcc aattggtttg ataatgggct aacggcgcga cagtgtaatc
    17881 gaggcgcccc tgaattggct ctcggaccgg atcaccacgg actgtttgtt gccgtgcgtg
    17941 gtcaaggaaa tattggcgct gaacccggcc gcgctcgcca ggacctgaaa actgccattg
    18001 ccaccgcgtc cctcgagggt tccgttgatg ctgcggctgg attcgctcca ggtcccggag
    18061 agcgatccgc cctccgccat gacattgctc ctgaggtcga atttgtagct atcgctggcg
    18121 cagagcaggt tctggttgag gccgttgccg ccagcaccca cggcataatt ggcgcgacat
    18181 cggatgcgct cggtcgatcc gtcatcgagc gtgacgatgc ctccgccgga ccagctgccg
    18241 gccatgccgg tgaacggccc cgactgggcg tagcttgcgg tgccggatcc cgacgccgcc
    18301 agcaaggcga tggctgcaag cagcagccac cgaaggcccg cgaaggctgt tattgttcgg
    18361 cccggtctat tgccgcgagg cttcccatcg gccgctgcac ggcacgcctg ccgacgcgcc
    18421 attccatttt cctgatcccg tattgccgct gagttgcccg tttgcatatg caccaccgat
    18481 cgaaaccctc acgagcccct cccgtccgat gctgccggtg acgtcagcgc cggcggccga
    18541 gactttgccg tccgcgaccg taagtgttga gcgcgtcgag gcttcgcaag aacctgcctt
    18601 ggtcacgatc gtgacattcc agagtccgtc atagggcgtc tgggccgcgg cgggaacggc
    18661 gctgagggca gcggcggcag aggtgacaac gaaagcacaa aatatcttac gaatgcgatt
    18721 gtggcgcatg ggtcaaatcc ccgatgtgtt tggtgatgca gccttagtct aggcacaatg
    18781 tgtctaaaat ttgctgcgcc gcgtcaacgc gaaaagttcc ctaaatatca aagatttgaa
    18841 gtcaaaattg gttcccggct gggaacagaa atttgttcca cgacggcccg tggacggagc
    18901 tttgagcgcc tgattcgaga aataggtatt atgttactcg agaagccagg ctatttcgga
    18961 agccgcggcg cgttggtcgc aaactgctcg tcctgggctt ttgccggcag ggcgttgtgg
    19021 attttcgcgt aatcgatgac ttccgccaaa agcttctggc cgagcggcgc cagtgcgcgc
    19081 tcccacagct cccgggcggt ctcgcccttc ttgacgaaaa cccactcctg cgcggcgatg
    19141 gcgccggcat ccatgcggtc ggcaaggtgg tagacggtac cgccggtgat cggatcgccc
    19201 tcccggatcg tccattccac cgccgcgatc ccccgatgcc gcggcagcag cgagggatga
    19261 tagccgatac ccccgagctt tgccgcggcc agcgcctccc tggcgatgcg ggcgtggctg
    19321 tgtgcggtca cgatcaggtc ggtccgatcg gcgatttcgc gggcgaccac gagcctgggg
    19381 tcagcctgta ccgtgacgtg gatgtcggcg gcacgggccg cggcggcgag ccggtcttcg
    19441 gcgtcggcca cgacgacccc ggcgatttcg acgccgtgct ggcgcagcat attgaatgtc
    19501 gtcacgccga aatggcggga gccgacgagg gtgatccgca tgttggctta tccgtcttgt
    19561 gcttaaggca tctgggccgt cgataacacg ccgcaggcag ctgtcatgcc gccggcagcg
    19621 gaggggtagc gggttatcaa caagaccggg gaaaatcgca aggcacgggc acggcgaggc
    19681 cagcgcgcgc acaacctaaa acaaagccgc caagcggaat atcatcgcct gacggcttcg
    19741 cagtggtggt cccggactcg ggggagatat ccggtacggg cgtgatagcg cgtttcgtcg
    19801 catccggtag tgaaccagtt cacatttggg cgtcgcgaat tgatctatga ggatgccagc
    19861 ccggatccgg gaggcagtta tgcaatcgct caatagccaa cgtcaaagca accacggcat
    19921 ccattggccg gcaatcattg cgaccgtgct ggtccagatt ctggttgtgc tggcgctctc
    19981 ggtcgcggct gctttctacc tggaatggtc atcggacgtt aatcaggccg aattcgccag
    20041 cgcgactcac cgatcatcgt tgtccgattc aaatcatctt ccggaagttt cgattccgat
    20101 tcgggcggtg cgaggtcggg cgggatgcga tccgaaggct caacgaaccg gcccgtaaac
    20161 ggacggcggc tttttcgcta accgaacgcg acaccgagcc gcttttcctt gcgccacacg
    20221 atgtaggcat gccggcgcat ctcgcagctg cggatattca gcgtgatctc gtggggaatg
    20281 cccgccgggc tcgcaacctc gaggccggcg ccgacggcgg agaggttgcg aacgatgcaa
    20341 tcgattcccg cgccgtcgaa ctcaatggtt ccggccttga atatgcgttg ccgcgggagt
    20401 gcacgtcgct caaccattcc atggatctgc gccaaaaagt ttaagagtcg ggtgaatcgc
    20461 ggcatcgaaa ccacggctga tctgaaacga tttggtaacc cacgccgact tcgcgcggtt
    20521 ccggcatgcc agcggtccaa tgacatggcg accatgatcc atgtccggta gcggacaaag
    20581 cgcagttcct tgatgcgatg atgagcctca tctttcgaac gaaagaaggc gcctagccga
    20641 ggcccaggca gcaaccggct aggtcaggtc gggttgcggg ggggggggac cgcctcaacc
    20701 ctcaaacaga tcccgtcgat ccgaacaaga gagttcgcgg agctctccga gcgttccgcg
    20761 cggcgcgccg cattgcctgc acttgatctg cgtcgagggc ggcgcgcctt ccgggcagtc
    20821 gaggacaatg ccgagggagt cgcacttgat gcagacaatt ctaaagcgag aatgccggcc
    20881 cgcaaacagc tcttccggca attcttgctg cgaacttcac ctgcccgggc ggtgaacttc
    20941 acctgtccgc cgtgcgcgcc cggctacttt gactgaatgg tgaaagcgga taaagcgggc
    21001 cgcggaagca gcgctcgtgc gacgagatag ggcatgaggc cgacgccgac aaaacccaag
    21061 atcatcatat ccccgctgga gcgctcggga tcttccgcaa cgggagttgc gtccaccacg
    21121 tagagttcgg cgccgaaggt gaacgagacg ccggtgagga gcgcccgggt gctggcgtca
    21181 gccgcgttgg gcgtgaaagt gcctgcgcga tcgatgacgg gaataaggta atcaaggccg
    21241 gtactttggc tggcagccgg accgcccctc atattgaatg gttctgcgaa cggttgcatg
    21301 tctgccacga atggttcggc ggtagccacc gtcgttccgc ccgcgccgcc agcgatcgca
    21361 tgcgacgtgc cgccgggtgg cgtctgcggg ccgacgcgat cgccgaggcc tgacagggaa
    21421 actgaaacgc tcttcagacc ggctgcgacg ctcgctgaca ccgcagtgtc agggtcggcg
    21481 cgggtgacga tgaagacggc aggatctgcg ttggcgatac cgtatagcaa cgccaggcac
    21541 gcaagtaacg cactggtact catatgaatt taaacgccta aaaggtattc aagtaaaata
    21601 tttgtatgat ttaacgatag taatgcaggc ctgtcaacga gcacagtaac cactcgctaa
    21661 aaccttaagt agtttatttg catagagcac ctcagaccag gatctctagg ctgacggaac
    21721 ggacgagcgg cggttttatc tgcagctaag cttgatcgaa cgcgacgcca atcggctttt
    21781 gcttgtgccg gcgccgcaca agccccgggg cggaccgctc cgcccggccg ggagcatgtg
    21841 gtcatccatc gtcgccgaac ggccccatgg ccccaagccg cacggtgagc aactcgactt
    21901 cggtgacggc gacgtgcgga ccttggcggg taccggccgg cgtcgtccag aatgtgccgg
    21961 cttcacagcg cctgccggcg gtttcgattg tgcccgacag caccagcacg aattcgtcgg
    22021 ctgggtgggc gtggaccggg atggtcgtgc cggcgcggag gcgcgcgcgg tgaatggacc
    22081 cgtcgggcac cggctgggcg agcggcatga gttcgacgcc cggcagaaag tcgatcgtca
    22141 gccacgtgcc ggcgcctgga tcggtgaagg tttgatggtt ggtcattgcg cggctccagt
    22201 aggagttgat aacctagagt tccgatcgaa ggtaacccaa ggttatgata tgaagcaagg
    22261 ctcacgcaaa agcggtgcga tcacgttggg agccatcgag gaggggcgcc gcaccaacat
    22321 gcggcagctg ctgctgcgtg cctcgcgtat cgtgaatcgg cacgtcgtgg aaggcttaca
    22381 tgcccgaggc tatgccgacc tgcgttccac tcacacgacg ctgctgtcca acatcgacct
    22441 ggccggcagc accgtgaccg tcgcggcgga ccgggcgggg atcaccaagc aggcgatggg
    22501 ccgacttgcg actgaactcg aaggcgctgg ctacatccgc gtacacggcg acccaaaaga
    22561 tgcccgcgcc cgcgtcctgc aactcaccaa aaccggcaaa cggctcatgc tcgacagcct
    22621 cgaggtcatg gccgaactgg agcgccgcta cgcccgttcg gtcggtcggg atcgacttgc
    22681 cgccgtactc cgaggtcttg cagcctttgt cgaaggggcg gcggagtggg attaggtaca
    22741 gaccctgggc ggggcacctg tccagcggtc gccgtcacct gcgccaaagc ttcacctgcg
    22801 cgtcggctcc ggatgtcgtg ctggttcgtc cggccgagtc ggcaatggtg gcctggaggg
    22861 gaggggtgtt ctggatggtt cttgttcgcg gcctgacatg cgatggctct ccgggagcca
    22921 cctaatacct gcggacagtc agtttctgca aggcaatttc tgcaaggcaa atagcggcgg
    22981 attgagcggt tctataggtt cgacctgacg gtgccgcgtg cgcggactgt ttgaaacgga
    23041 aggccgttcg ccgcgctcaa tgcgctggtg gctgtcgcca gcaggcaggt gaacccttga
    23101 tctgtgaagt cgaccacaga gtggaccgca gcgccgtgtc atcttgttcc acggagggcg
    23161 atcccatgta cgtcatcaac gctgggccac atcgcggatg atggcgcttg acgatgatga
    23221 gaccgcgcgc tgcgtattcc tgacccacac agtagtaggg gtggcgtggc tacgctgctc
    23281 aacattgctc agtcgagcgc tgccctgtgg tggtcgcttc gagcggcggc atgacttgac
    23341 ttccctgagt tcagctactt tcgaggcctg cctcgttttg caaagatcaa ttcctgatgg
    23401 tcagccgttc tacgaagaag gccggctttt ttcagcgcct cggttcgccg cgctccgggc
    23461 ggcatctcgc gcgcttcctc aagcgcgtcc gccgctcgcg cgctatgact gcggtgatca
    23521 gcggagattc cgccttcctg cttctcgccc acctacttct cgtccatgtc ttattggggc
    23581 ggcttgagtt cgccgccaaa ccagccgcga acgttggatt cctttgggtc cctcgatgca
    23641 gtcgaggtct ggacacgacc gcacttctgg cactcgaatg tccggcggtc gcagccggag
    23701 ggtccgccct cgatcctgga gagaagcatt cgcgggtgat tgcagatcgg gcagcgaggc
    23761 cgcttgatcg cggcgaggtg ggatgggtag acaggctgat attccggcat ggcaattccc
    23821 cgcggcggga gggttacgaa acacttggtc acagacgacc tgccgagaca ggacggtgat
    23881 caacccacca tgtcggcgcg gagatctgac tcgctggtca atactgctca ttttccaatt
    23941 ttaatttgat cagcgtcctg tccgcggcgg gggatgcggg accgtttgcc gcgaaaggcg
    24001 gtggattgcg gtgaaggagg gccaccgccg ccatattagc accacgatgc gcaccaaccg
    24061 tgacagggta tgggccgggc gaagtcgaac gcgtggggac gccgatgacg acgattcaag
    24121 ccgtggtttt tggggtgatg ctggcattga cgccgagcat gcttgtgctt gcgtttctgc
    24181 tgtggcgaga gggaattgga cttgccgacg acggttccaa tggcgaagcc tattttcatc
    24241 gcaaatatta gagcttgacg atcaaccacc tgatcccaag gcccaataac cagatccagc
    24301 cgcccgtcga gatgatgatg gcaaccagaa aggttatctt caaaatcaat cgacgcattt
    24361 tatttatttg cgaactgact atttgcgaac tgacttcggc tgtcggccga tgatcttccg
    24421 gtcgccctga accgcttgtg aatgggcttg tccagttgcg gcgccccagt cggaatggga
    24481 tgtcgtggcg gctttgcccg attacggatg gtcgtagcgg gtcgccggtt tgtcccgtac
    24541 gtcatttgtc gacggtgcca tcgtctgcct ggtttcgact tcgcgttcat gaaggcgttc
    24601 caggatggcc gtgcgccttg gtatctgcgc agcctcatcc gcaaaccgct gcgcgagatc
    24661 ggtgtgatat tgtcgcacga tgtcgagcgt tgctttcttg gcgatactgc gatggtacct
    24721 ggcaagtacc ccgtgcatcg ctgcgagcag ttcatcggtc atggctattg ctgcttttca
    24781 gtctgttact ggctttcgac gacctgaaaa aagcagtagc cgcgcatgcc gacgtctcga
    24841 gccgcgtccg aatgcaccgg agattttcca ttcggcatca gcacccactc cttgcgcttg
    24901 agatgcgcag gcagcccggc gcccggcatc gtggccaggc gcaaggttcg atcggcggtt
    24961 ccgcgataga tgtcgtaggt gatcttgtcg gtcatcaaat gaggatagcg agacaacccc
    25021 tcgccgcaat tgcatccgtg gcttaacctt accggttaag gttgcacggt ggccggtcgc
    25081 cgaactgatt tttgtccgta gcgcgaaacc ctggccatgt cccgatccat cctgaggatg
    25141 ctcggcgata aagactgcgg ccgatcacgg cgccgcgcca gcgaggctgt caccaatcct
    25201 ccggttcgcc gcgcttgtgg tcggacagcg cgtcctcgtt caattcgatc aggtctcgca
    25261 ccgcctggac atgaatcgcc agcgcctggc ggcgctgtga cggatgatgg cccatgatcc
    25321 accgcgatac gagatcggtc agcacggcgc gctggaggtc atggcccttg ccatgcaaca
    25381 gccgtccgat cgcatgtatc agcgcatcga attcgtcatc gacctttcgc ccaaagatac
    25441 ccatcgtcgc ttaccgttct cttggaagaa ccgcaacgta cgaagcctgg ccgcgaactc
    25501 cggcattttg caactttccg atttgcctgg tcaagccggc cttgaacgcg ggggagcgac
    25561 tttcgtgccc cgcctggctt cactgcgttg cagcatgcgc gtcggttccg cctgatgggc
    25621 ggctccagcc gcggccgcgg gcgtatcagg aaacctagcc tggcgtgctg acgaaggtgg
    25681 ttgccttggc ggctaaaagc ggcggtcggt ggccggcgat tgagtgacag gtctttaaaa
    25741 ccgccgatca gaatttatcg gcagggacgg acccgtccgt cgtagcccct gtaggttccg
    25801 gatgctgcat cgtaggtccg atagcgctgg cagccgctca agctcacatg ctcgcgcagc
    25861 ttgcgctcgg cacgctttgg caatggaact gtttcgaacg gtttatctac cggctggctg
    25921 ttctccggcg ccaccggtgc catggcctcc gattgcgtga cggcgggctt gtcttccacg
    25981 gcggcggccg gtttctcttt cacggcagcg ggtttctgct ccacgacgtc ggcgggtttt
    26041 ggctccactg cagcggagtt ctgctccacg tccttgccgc ggtgctgttc gatgaactcc
    26101 ttgcattgaa tcgggaaaac gatttctccg gaagtggtca gcccgatcgg catacagccg
    26161 ccgggtggca cgtctgcatt cccggaaggt tgcgctgaag cggcgctggg attcactgcg
    26221 ccagttgtct gggccagagc tgcgctgccc gtgaccgaaa caaggagagc caataaaaaa
    26281 cacttcatgc gatctctcaa ccaacgttcc ggggaatctt ccatcccctg cacggagatc
    26341 ggcgtcatgt cgcgccacga tccgccgtcg aacccatgcg cgacggctcc ggcggagaca
    26401 tcaggcgcga cggcacctaa agcctcgccg ctccacaggg cagcgccgaa tagattgcgc
    26461 cggcgaccat gatcagcgca agaataatga ctatggtttc atgaccccgc cattttatcg
    26521 cgcccgattg tggccaaatc ggtcaaggat ctcggcaaaa gttgggacgc gctgcggcag
    26581 gccctccgtg cacggacaca tggttaatag gacacccgtg ttgtccttaa aaacctggaa
    26641 ttatatgcaa tcttcggtgt ttaaatgctc ggcccacgag taatatttgg gcggggcaac
    26701 gggctggata tgatcggaga cggggcgccc ccatgcgcgt cgcagtaact gttgtgtttg
    26761 cgttgatggt ggcgacacca tcggaggcgt caaagtcctg catgagcaag accgaagcgc
    26821 gccagcattt tggttcggtg catatctatt ggcacggccc ggcgcattgc tgggacgaca
    26881 cgtcgacacg tcggcaccat cagatccacc agatccatca ggttcggcac aagaaaaatg
    26941 atccgaactg gcatgacgcg atgtcggaga tgccgcctga tggcgggtct gcgtctgcgt
    27001 cgacacgact tgcgcctgtc ccgaaacaac ttgccgctgc gccgacacaa cttgccgctg
    27061 tgccgacaca acatgcgcct gcgccgacac catgggtcaa tcgctgggtg gatatcgagc
    27121 aatctcccat tagctcgcgc tgggttgata tcgctcaggt cgcgccgccg ctggtcatcc
    27181 gcgcagccga gccgctggtg acgccgcgcg gcgtggtgat ggtgatcatg ttcgtcatcg
    27241 ggagcctagc gatcatcata ttccttcaac catcgagaag aaattggcgt tcgacaggtt
    27301 agggcgctct ccaacagtca ggtgtcggcg ctgggctccg gcaatttcaa cctggatttc
    27361 aacttggcct gaaaggcggc gtcgccacca ccatcataaa ctttgaagat gagccatatt
    27421 gaccagcgtc ccgggaaccc gaagcgcatg atggttccgc gccggcatca cccggtgact
    27481 gagtcggata ggtggctgcg tctgttgctc gtctgacgta agtgccgttg aaggccggct
    27541 cagtggatgg cccgtggccc ctggcgcgcc cggtcgtcgt ccacggcatc aagagcccgt
    27601 tggtctcttc ttcggccaat agcctcatca gcatgctgtg acgtatttca tcttccgacg
    27661 tggcaagttc ggcttcggct agcagccgtc gataatgcgc caggttttgc tgatggataa
    27721 aatttcccat gacaaggctc ccccgatatc gccgtctcct tacgccggat tgcgttgaca
    27781 tgctgtgcca taatgctcag cgagcgaagt tttccccgcc attcgttccg ctggttggaa
    27841 ggctatcgat taccgcggct gcacgaccgg gatgacggca gccagggtgt caaacactgc
    27901 cgtgatcgat cccgcgcctg cttgcggcgg tccccttgtg ctagtcgact gccgaaagtg
    27961 cttctcgttg caactagtga aacgcgtagt gcagctttcg cttgaagttg agaagtgcga
    28021 aatggaggcg cgtgcggatc gggtgtcgct gcatttctct ctgcatggcc atctgcttgc
    28081 gcatcatcat ctgtttttgc atcatcattt tctgctgagc catgccttga cgccttgccg
    28141 cgagagcgct ggcgtcgggg cgctgcgccg acgacggcag gctcgcttcg ggagccctga
    28201 catcggtatt ggccgcggtg acggccagtc cggcatgagc cgccgtggca gacaaaagaa
    28261 caactgcaat caggagaaac ttgcgcatcg tcgcctccat ttgatgatgg cgacagtctc
    28321 tcccatccag gcacgaaatt atgtgatccg gatcaccctt gccgccctcg cgcggatcac
    28381 cgtgcgtgcc ccgggcagtt gctcccgcct gatcggggat gcgccatgtc ggaatcaaac
    28441 tccattccac caacctgctt ttcccatatt aagtcccata tcgagcggcc ccgctgtcca
    28501 aggtgccacg aaccacgcat gcttcattcg aaaaccgagg cgggaccggc aggttcgaat
    28561 accgcacgtt cgactgccag aagtgcagtc gtactcacac gatgattatt gcgagcgatc
    28621 caacggattc caacgtgcgc cgttggcgcc cggcgcccgt cacgcacgct cgcgaaacga
    28681 cgacggcctc agctatcgca gtctcccgcc aagctgaggc cgtcactttg ttggcatcgg
    28741 tcgaaacgag cagggctgcc cacgatccgg atgacgacgc tgcaaagcgt gcgtttccgg
    28801 gacccgctag tcaagttgct cagttatcaa aatgaaacga atttgccgaa agcggcctac
    28861 tcaaagtcgg gctgcgaggc gtcgcctagc atcttggcgg cttgatccac aacgtctcga
    28921 tcgggcccga caggggcctc atcggcgcgg gcgacgggcg gcctgtcgtg ttgccgcacg
    28981 atcgaaagcc gacacgcatc gtgtgacgcg agagcttccg tcagtttccg caggcaacga
    29041 accaattcct cttgggaact catattccgt gaaccctttt tccagcatcg cccggttgcg
    29101 gctgacaagt gcgattggct caccatggcg ctggaatgcc ctgatgtcgg gcatcgcgaa
    29161 cctcgccact ccgatcgagg tgagggcagc gtccagcggc atcgtgctgc ggaccctcaa
    29221 actacccgct cttgctcggc agcttgatcg gaacgtgccg cgaggtgctg acgacgatcg
    29281 agcttccgtc ggggcgggtg cggctaatga tcgtttcaag gaccagaata aacttctcag
    29341 caagcatgtg cgttgcctcg tttgttggct tcgcacgatg gatagcctgg atgacggcca
    29401 acaaaggtga ccgggctcac accgtggcgc tggaatgcca atcgtgcgga ggagctggcg
    29461 gaccgcgaga taatggaatc ggattgcgaa gcctcaacag cagcccatga agggcttaac
    29521 ccctagacgt ggcggtcaag ctgcggctcg aggccggcgc gttcagccag cccgcgcgtt
    29581 tgggtgcgcg taagtttgtt tcgcagccag cgtgcggaat ccgaggcaaa gcggccagcg
    29641 ccggcaagga acctcaatca acgcccaggt tacgctggat gcgacgataa ccgcgccgat
    29701 ggccatcgcc
//
LOCUS       DATNOI010000250         6492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1460230,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000250 DATNOI010000000
VERSION     DATNOI010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460230"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /gene="opgC"
                     /locus_tag="VN968_09730"
     CDS             complement(<1..376)
                     /gene="opgC"
                     /locus_tag="VN968_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HXL29551.1"
                     /translation="MVNQDRAPFSHAVERDLRLDLFRGMGLWMIFLDHIPHDIVAWLT
                     LRNYGFSDAAEFFVFISGYLAGFIYGPIVRGGHFLAATKRLVMRAWQMYVAHIMLFLL
                     FTAQIARTVRKFDNPMYEDEFNV"
     gene            869..1330
                     /locus_tag="VN968_09735"
     CDS             869..1330
                     /locus_tag="VN968_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXL29552.1"
                     /translation="MNINRRELMLPALALGLLGVIPASMVPAFAGADEDAVAKNLEAF
                     RAAQAVGNAEALAGLCAAELSYSHSSGKVEDKATLLAGVTNAKYKWTSLEYKNPTIRI
                     VGPAAIVRFNFVGEQEFTADGKKVPQNLHILMNWQKQGADWKLLSRSATAL"
     gene            complement(1480..1671)
                     /locus_tag="VN968_09740"
     CDS             complement(1480..1671)
                     /locus_tag="VN968_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29553.1"
                     /translation="MSDSYIIEVGSKTAGIVVRNPEGYRFFAATNRFNALEGQLFRNA
                     REAERAAVRLANGNLELAA"
     gene            complement(1903..2337)
                     /locus_tag="VN968_09745"
     CDS             complement(1903..2337)
                     /locus_tag="VN968_09745"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HXL29554.1"
                     /translation="MTDSQAVHAPLNTENEPCGDLSIRTMAMPADTNQNGDIFGGWLL
                     SQMDIGGGIFASKMAKSRTVTVAIEAMNFRKPVFVGDVVSVHADLVRIGKTSITVHVE
                     AWVLRRKETHSILVTDGNFTYVSIDVQGHPQVVKRDQPPISA"
     gene            complement(2363..4459)
                     /locus_tag="VN968_09750"
     CDS             complement(2363..4459)
                     /locus_tag="VN968_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXL29555.1"
                     /translation="MESRIMNVLADRVLELGPKPDADSPYRHFKLTRDSDGIAWLLFD
                     REGASANTLPADVMEEFDTVLAALENQRPTGLVIRSAKKSGFIAGADVNEFRGATDPQ
                     AVEAAIGRAHAVIDRLQALKIPTVAVIHGFCLGGGLEVALACQSRIAIDGARFGFPEV
                     MLGLHPGLGGTARFTRLVNPMQAMTLMLTGKTVDARKAKSLGLADAVTQERHVRNAVN
                     DAVFGRLKRARPGLLNTILNLGPARGFLASRMRREAEKAAPHEHYPAPYALIDLWEKH
                     GGDKAAMLTAEKTSFANLMVTPTAQNLIRVFFLREQMKKLAGSGNRIGQVHVIGAGAM
                     GGDIAAWSAGQGLRASIGDVKPEPIAGAIKRASDLFGKIMRKPTDIRDALDRLTPDLD
                     GEGVRNADLIIEAVPEKLELKQKVYASLEPKLKPGAILATNTSSIPLEDLRSTLQSPR
                     RLLGLHFFNPVSRLQLVEVISHDGTDPTMLKQALAFVGAIDRLPLPVRSSPGFLVNRA
                     LTPYMLEAMVMLDERIDKLAIDAAAEKFGMPMGPIELADQVGLDICLAVGDMLRSKFG
                     DLLPPTPAWLREKVAKGELGRKTGKGFYIWKGGKPDKSSRLPDTAQPTDEMIDRLILP
                     ISNVCVACLREGIVDNPDMVDGAMIFGTGYAPFRGGPLNYARTRGVDNVVSTLHALAQ
                     KFGGRFAPDAGWDSFK"
     gene            complement(4462..5745)
                     /locus_tag="VN968_09755"
     CDS             complement(4462..5745)
                     /locus_tag="VN968_09755"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXL29556.1"
                     /translation="MARPVFIIDGSRTPFLKARSGPGPFTPVDLAVQCGRPLLARQPF
                     APTAFDQVILGCVNVIADEMNPARVAALRLGMGEAMVAFTMQINCGSGMQSIDTGYRY
                     IREGISNLILAGGAEALSHAPLVWPQQGVRWFAGLAGAKGILARLVALAKVRPSYFKP
                     IIGLERGLTDPITELNMGQTAEVVGHLFGITRAQSDAYAAESHKRLANAQAQGFLKGE
                     VETAFARDGRFYDHDDGVRPDSTPETLAKLKPVFERPWGQVTAGNSSQITDGASWTIL
                     ASEDAVKKHGLTPRAVIVDSQWAALDPSIMGLGPVLSATELLKRNKLALGDIETWELN
                     EAFATQVLGCLAAWNDEKFCREILGLDGAAGQIDHAKLNVDGGAISLGHPVGTSGNRI
                     VLHLVNAMKRLGTRRGIATECIGGGQGGAMLIETV"
     gene            complement(5745..>6492)
                     /locus_tag="VN968_09760"
     CDS             complement(5745..>6492)
                     /locus_tag="VN968_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase domain-containing
                     protein"
                     /protein_id="HXL29557.1"
                     /translation="APAPDAGAATRFYRQLSRYSSAFALCADMALLTLGGALKRKEML
                     SARFGDILSELYLLSAALKRWQDEGRQQADFAALEWCMASGFRTIENRFSEILANLPN
                     RFVAVILKFLVQPFGARVLGPSDRVVQQCAELVLEPSAARDRLTPDLSLIDDDRGVAR
                     LEKAFVLVTGTEDIARRMRAADIHDWREAVKQGVITQAEGDQLAVAHEAVVKVIEVDD
                     FPPEALSPQSNKPAEVHRFFQELGEQRAAS"
BASE COUNT         1264 a         2026 c         1993 g         1209 t
ORIGIN      
        1 gaacattgaa ttcatcctca tacatcgggt tgtcgaactt cctgaccgta cgggcgatct
       61 gtgccgtaaa gagcaggaac agcatgatgt gggcaacata catttgccag gcccgcatca
      121 ccagccgctt ggtggcggcg agaaaatggc caccgcgtac aatcggaccg tagatgaatc
      181 cggcgagata gccggaaatg aacacgaaga actcggcagc atcgctgaag ccgtagttgc
      241 gcagggtcaa ccaggcaacg atgtcgtgcg gaatgtgatc gaggaaaatc atccacagcc
      301 ccatgccgcg gaagagatcg agccggaggt cgcgctcgac ggcgtgcgag aaaggcgccc
      361 tatcctgatt gaccatcgtg ccttatccat tttgtcgtgt tgggcatctt gtcttgttgg
      421 gtatcgcgcc aagggtagac aggcgacatc ggaggtgttg cgaaagaccg cgccgccaag
      481 caatcaatgc gttcacggtt cgcagtcgat ggctttaagg aaaccgtggg ctagaaacac
      541 atcctttggt cgattgacga acgcttattg agaccttcca ggataccggc gtcaatgtcg
      601 gggatggtca aaagcaccgt cttgactcgc tccccatcac gtccggtcta ctccgaaatc
      661 tgatacgaag cgatccagag caagcaacgg cctggattgc ttcgctaagc ctgtcatcgg
      721 acgcgcattc gcgcgacccg tggctcgcaa tggcggggac gttcgcaaac gcaacatcgt
      781 gcgcggtctt tcgcggggcc aaagcgggtg ctcgacaaaa gcgagcgcga ctgcaagact
      841 gcctacaacc ggaacaaggg aggaagcgat gaatatcaat cgtcgtgagc tgatgttgcc
      901 ggcgcttgcg ctgggattac tgggcgtgat tccggcgagc atggtcccgg ccttcgctgg
      961 cgccgatgaa gatgccgttg cgaagaatct cgaggccttt cgcgcggcgc aggcggtcgg
     1021 caatgccgag gcgctcgccg ggctctgcgc ggccgaactc agctacagcc actccagcgg
     1081 caaggtcgag gacaaggcga ctctcctggc gggcgtcacc aacgcaaaat acaaatggac
     1141 gtcgcttgaa tataagaatc ccacgatccg tatcgtcggt ccagccgcca tcgtgcgctt
     1201 caactttgtc ggcgagcagg aatttacggc cgacggcaag aaagttcctc agaacctcca
     1261 catcttgatg aactggcaaa agcagggcgc ggactggaag cttctgtccc gatcggccac
     1321 cgcgctctga tcgcggccgt catagcggcg aaagccggca atcgcgtaat ctgaagcgca
     1381 agcgatgcgg cggggttgat cggagcggtt gcgaccgcac cgaagcgatg cggcttgcaa
     1441 gagtggcccg ggataaccgc tgagtccgca gcatgacagc tatgcggcta gctcgagatt
     1501 accgttggcc aggcgaacgg ctgcccgctc ggcctcgcgt gcattgcgaa acaactggcc
     1561 ttccaatgca ttgaagcgat tcgtggccgc gaaaaaccgg tagccctccg gattccgcac
     1621 cacgattccc gccgtcttcg agccgacttc gatgatgtaa ctgtccgaca tttctactcc
     1681 gcgcccttat tcctcagtgc gggccaataa ccatgcaggc gctgaaaagt tccggggcga
     1741 gaaatcacga gactcagggc gtcacaacac caaatcgtca tcaccgggct tgtcccggtg
     1801 ttcgacattt tactttccca gagggagaca aagacatgga tcgccgggac atctcgtgcg
     1861 aagacgcttc gcgctcttgc ccggccgcga gggggaaatt tactacgccg aaatcggtgg
     1921 ctggtcccgc ttgacgactt gcgggtgtcc ctgaacgtcg atcgagacgt aggtaaaatt
     1981 gccgtcggtc accaggatcg aatgtgtttc cttgcggcgc aaaacccatg cttcgacgtg
     2041 aaccgtgatc gaggtttttc cgatccgaac cagatcggcg tggacggaaa ccacgtcgcc
     2101 gacgaacacc ggtttgcgaa aattcatcgc ctcgatcgcc accgtgaccg tgcgcgattt
     2161 tgccatcttg gacgcaaaga tgccgccgcc gatatccatc tggctgagca gccagccgcc
     2221 gaaaatgtcg ccgttctggt tggtgtcggc tggcatcgcc atcgtgcgga tcgacaggtc
     2281 gccgcagggc tcgttttccg tattcaatgg ggcgtgaacg gcttggctgt cggtcatggt
     2341 ggagcccctg acaaaaatcg gattatttga agctatccca gcccgcatcc ggcgcaaaac
     2401 ggccaccgaa tttctgcgcc agcgcgtgca aggtcgagac cacgttgtcc accccgcggg
     2461 tccgcgcgta attcagcggg ccgccgcgga acggcgcgta accagtgcca aagatcatag
     2521 cgccatcgac catgtcggga ttgtcgacaa tgccctcgcg caaacaggcg acgcagacat
     2581 tcgagatcgg caggatcagc cggtcgatca tttcgtccgt cggctgcgct gtgtctggca
     2641 aacgggagct cttgtcgggc ttgccgccct tccagatgta aaaacccttg ccggtcttgc
     2701 ggccgagctc gccttttgcg actttctcgc gcagccacgc cggcgtcggc ggcaacaggt
     2761 cgccgaactt cgagcgcaac atgtcgccga cggcaaggca gatgtcgagg ccgacctgat
     2821 cggcgagctc gatcgggccc atcggcatgc cgaatttttc cgccgccgca tcgatggcaa
     2881 gcttgtcgat cctctcatcc agcatcacca tcgcctcgag catgtagggc gtcagcgcgc
     2941 ggttgacgag gaagcccggc gaactcctga ccggcagtgg cagccggtcg atggcgccga
     3001 cgaaggccag cgcctgttta agcatcgtgg gatcggtgcc gtcatggctg atgacctcga
     3061 ccagttgcag ccgcgagacc gggttgaaga agtgcaaccc cagcaggcgc cgcggcgatt
     3121 gcaacgtcga gcgcaggtct tccaatggaa tgctcgaagt attggtggcg aggatcgcgc
     3181 ccggcttcag tttcggctcg agggaggcat agaccttctg cttcagctcc agcttttccg
     3241 gaaccgcttc gatgatgaga tcggcgttgc gcacgccctc gccgtcgagg tcgggcgtca
     3301 gccggtccaa cgcgtcacgg atgtcggtgg gcttgcgcat gatcttgccg aacagatcgg
     3361 aagcgcgctt gatcgcgccg gcaatcggct ccggctttac gtcgccgatc gaggcccgca
     3421 ggccctgacc ggcagaccat gccgcaatat cgccgcccat cgcaccggcg ccgatgacgt
     3481 gaacctggcc gatcctgtta ccggagcctg cgagcttctt catctgctcg cgcagaaaga
     3541 acacccggat caggttttgt gccgtcggcg tcaccatcag gttcgcgaac gaggtctttt
     3601 cggccgtcag catcgcggcc ttgtcgccgc cgtgcttctc ccagagatcg atcaacgcgt
     3661 aaggcgccgg atagtgctca tggggcgcgg ctttttcggc ctctctgcgc atgcgcgagg
     3721 cgagaaagcc tctcgcagga ccgagattca ggattgtgtt cagcagcccg ggccgggcgc
     3781 gtttcagccg gccgaacacc gcgtcgttga cggcattgcg gacgtggcgc tcctgcgtca
     3841 cggcatcggc cagcccgagc gactttgcct tccgcgcatc gacggtcttg ccggtcagca
     3901 tcagcgtcat cgcctgcatc ggattgacga gacgcgtgaa gcgcgcggtg ccgccgagac
     3961 cgggatgcag gccgagcatc acctccggaa aaccaaagcg ggcgccgtcg atcgcaatac
     4021 gtgactggca ggcaagcgcg acttcgagcc cgccaccgag gcagaagccg tgaatcaccg
     4081 cgacggtcgg aatcttcaaa gcttgaagcc ggtcgatcac cgcatgcgcc cggccgatcg
     4141 cggcttcgac agcttgcgga tcggtcgcgc cgcgaaattc attgacgtca gcgccggcga
     4201 tgaagccgga tttcttcgcc gagcgtatga caaggccggt cgggcgctgg ttttccagcg
     4261 ccgccagcac cgtgtcgaat tcctccatga catcggccgg cagcgtattg gcgctggcgc
     4321 cttcacggtc gaacagcagc caggcaatgc catcgctatc gcgggtcagc ttgaaatggc
     4381 ggtaggggct atcagcatcc ggcttcggcc caagctcgag cacgcggtcg gcgagaacgt
     4441 tcatgatcct tgattccatg atcacaccgt ctctatcagc atggcacccc cctgcccgcc
     4501 gccgatgcat tcggtggcga tgccgcgcct ggtccccaat cgtttcatcg cattgacgag
     4561 atgcagcacg atacggttgc cgctggtccc caccggatgc ccgaggctga tcgcgccgcc
     4621 gtcgacgttg agctttgcgt gatcgatctg cccggcggcg ccatcaagtc ccagaatctc
     4681 gcggcagaat ttttcgtcgt tccaggccgc aagacacccc agcacctggg tcgcgaatgc
     4741 ctcgttcaat tcccaggtct cgatatcccc gagcgccaat ttgttccgct ttaaaagctc
     4801 ggtcgccgac agcaccgggc cgaggcccat gatagaggga tcgagcgcgg cccattgact
     4861 gtcgacgatc acggccctgg gcgtcaggcc gtgctttttg accgcgtctt ccgacgcaag
     4921 gatggtccat gacgcgccgt cggtgatctg cgaggaattg ccggcggtga cttggcccca
     4981 gggccgctcg aataccggct tcagtttggc caaggtttcg ggcgtggaat ccggccgcac
     5041 gccgtcgtcg tggtcgtaga atctaccgtc gcgggcgaac gcggtctcga cctcaccctt
     5101 gagaaaaccc tgcgcttgcg cgttggcaag ccgcttgtgg ctttcggcgg cataggcatc
     5161 cgactgcgcg cgcgtgatgc cgaagaggtg accgaccact tcggccgtct ggcccatgtt
     5221 gagctcggtg atcggatcgg tcagtccgcg ctcgagccca atgatcggct tgaaatagga
     5281 ggggcgcact tttgcgagcg ccacgagcct ggccaaaatc cccttcgcgc cggcgagccc
     5341 ggcaaaccag cgcacgccct gctgcggcca caccagcggc gcgtgactga gcgcttccgc
     5401 cccgccggcg aggatcaagt tggaaatgcc ttcgcggata taacggtagc cggtgtcgat
     5461 cgattgcatg ccggagccgc aattgatctg catggtgaat gccaccatcg cttcgcccat
     5521 gccgagccga agggcggcga cacgcgccgg gttcatctcg tcggcgatga cgttgacgca
     5581 gccgaggatg acctgatcga acgcggtagg tgcaaacggt tgccgggcca atagcggccg
     5641 gccgcactgc acggcgaggt cgaccggggt gaacggcccc ggcccactgc gcgctttaag
     5701 aaatggcgtc cggctgccgt cgatgatgaa aaccggtcgc gccatcagct cgccgccctc
     5761 tgctcaccaa gttcctggaa aaaccgatgc acttcggccg gtttgttgga ttgcggtgac
     5821 agcgcctccg gcgggaagtc gtcgacttca atcaccttca ctaccgcttc atgcgccacc
     5881 gccagctgat cgccctcggc ctgggtaatg acgccctgct tcaccgcctc acgccagtcg
     5941 tgaatatcgg cggcgcgcat tctcctggcg atatcttcgg tgccggtcac cagaacaaac
     6001 gccttctcca gccgcgccac cccgcggtca tcatcgatga gcgacaggtc aggggtcagg
     6061 cggtcacgcg ccgccgacgg ctccagcaca agctccgcgc attgctggac cacacggtcg
     6121 gaaggaccga gcacgcgggc gccgaacggc tggaccagga atttcaggat gaccgcgaca
     6181 aaccggttcg gtaaattcgc gagaatttcc gagaagcggt tttcgatggt gcggaagccg
     6241 ctcgccatgc accattcgag cgcagcgaaa tcggcttgct ggcggccctc gtcctgccag
     6301 cgtttcagcg cggcagacag caaatatagt tcggacagga tatcgccgaa acgcgccgac
     6361 agcatttcct tgcgttttag cgcgccgcct agcgtcagca gcgccatgtc ggcgcacaac
     6421 gcgaaggccg acgagtagcg cgagagctga cgatagaatc gggtcgccgc tccggcatcg
     6481 ggcgccggcg cg
//
LOCUS       DATNOI010000251         5593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1985383,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000251 DATNOI010000000
VERSION     DATNOI010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985383"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..1131)
                     /locus_tag="VN968_09765"
     CDS             complement(277..1131)
                     /locus_tag="VN968_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29558.1"
                     /translation="MQQRFHAWLSDNPDQAILRWIFRSVVTVTIAVLALDLISMNGWI
                     ANPDAAATPAELKQGSPTLDLPSLVPSILAPLLPGGDKRLLPLLQPQGAMAKPMTFEL
                     VGGGKLMATGTITPGISEAFAAEVSRRGDYIKTVALNSPGGSVADALTMGRLIRERRF
                     STEVEAGKYCASSCPLVFAGGVERRAADKAIIGVHQVAAISSAASGPLRDEMSVAQNI
                     SARCQRYLGDMGVNLQVWVHAMETPHDKLFVFKPDELKLLNMVTVAPASAPVPAPATL
                     SAPTKTRS"
     gene            complement(1159..2505)
                     /locus_tag="VN968_09770"
     CDS             complement(1159..2505)
                     /locus_tag="VN968_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29559.1"
                     /translation="MPPNNTLASQAPPSRSASALLSNGVTRLEGMLKLTTAILALAAG
                     VYTFLGVRELLNGSPTTVFFAAVIYSVAVSVGIYAFWVFLMQFMPHVIDRTGRSLMFG
                     CMLLGSLMIVAMSSWLNASALAGAAAIQQHLAITVQNYTRDLDTANTNAIAAQGLLPD
                     IQLASSRFAKLADAERAGSLTGTAGSGTVVQLLTQMSGQLDSLGQEVQASGKRVSALY
                     EQGGKSLARMRELISDRGPISTRSDAFATESLALMGVIANLQQTSVAPAVKRAATSLS
                     SGFIAPAAGGRTSDLADRQTAVVGKVESSIAAQAASLSDAADKILATPRIEPIRFQTL
                     SPAEAVLRYAGDFIPSWAGAISIDLMPAVLVLILCVVHAAIRREGLPAVSASNMTAGE
                     LLAALQMAREVEDARRRSPNEEVSPAEVKTDGQPVAEPAAAADENVTSLSSARLTK"
     gene            2643..2960
                     /locus_tag="VN968_09775"
     CDS             2643..2960
                     /locus_tag="VN968_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29560.1"
                     /translation="MATNNVLTDVIAEFGRKYLWWEPVSGQPHSEDRIITQTMNLGTY
                     DDILLLEEAVGKARLLEVMLRAEPGWINDRSWEFWRGRLSFATGASIPEKGPRRAFHA
                     ATP"
     gene            2944..3609
                     /locus_tag="VN968_09780"
     CDS             2944..3609
                     /locus_tag="VN968_09780"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HXL29561.1"
                     /translation="MPRHLEPKLSILPAAQRQIWTSLAPALQLSFVLYGGTAVALHLG
                     HRESLDFDFFRYEPLDKDQVRDAFGFVRGAAILQDSPDTLVVLAEMPSGLVKVSFFGG
                     IGFGCVNDPLQTRDGTLLVASLDDLMATKLKATLDRAEAKDYRDIAEMISAGVSLPAG
                     LSAFKQMFNGEPAQVLRALGYFEDGDLATLDKADQNLLRNARDKIQRLPRVIVKSGSL
                     TGR"
     gene            complement(3625..4518)
                     /locus_tag="VN968_09785"
     CDS             complement(3625..4518)
                     /locus_tag="VN968_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL29562.1"
                     /translation="MTTILEKTAGLVAFVQTVDSGSFSGAGRFIGSSPSAVSKSVARL
                     ERRLGVRLLQRSTRTLSLTAEGAAYYERVAPLLRAIADAEDVVQMADNARGLLRVTAP
                     VFVGRTLIGAWSEAFLARHPNVKLELSVTDRQVDLIREGFDVALRIGELQDTELTARK
                     LANLSMVIVASPDYLARRGVPRAIEDLRGHACLRYLLAGRSYPFMFADGTSLTPDGPL
                     DSDDGITLFHAALHGAGIAQVLRFAAEDDLAAGRLVEILPDLAMPKVPMHVVHAFGRQ
                     LPVRARLFIDFLVERMGPLGG"
     gene            4634..5383
                     /locus_tag="VN968_09790"
     CDS             4634..5383
                     /locus_tag="VN968_09790"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016555499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXL29563.1"
                     /translation="MSDLKGKVAIVTGASKGIGAGIAKGFAAAGAAVVVNYASSREGA
                     DRVVADITAQGGRAIAVQGDVSKAADVKRLFEQTKVSFGALDVLVNNAGVYQFDPLET
                     VTEKEFHREFDTNVLGTILTIQEALKYFGPHGGSVINISSIASLNPTPDSVVYAATKS
                     AVDSITRGLSKELGARKIRVNAIAPGGTASEGLVTAGILGSDFEKQIIGMTPLGRFGQ
                     PEDIARVAVFLASDDSSWLTGERITASGGWR"
BASE COUNT         1100 a         1686 c         1784 g         1023 t
ORIGIN      
        1 aatatatccc ggacctcatc ctgaggagcg cgcctttgcg cgcgtctcga aggatggccg
       61 cgagtccgcg ctttgcgtcc atccttcgag acgcttgctt cgcaagctcc tcaggatgag
      121 gtcggagata tatttcacaa cctcgttcgc cgggacccac gccgtgccgc cagatgcttt
      181 gcacggtgct tgttgcagat gtctcgatga aggaaatgcc ggtggttatg gatcccggcg
      241 ttcgccggga cgacccgcgg agaggcggcg cccaaatcac gatcgcgtct tcgttggtgc
      301 cgataacgtc gcaggtgcgg gtaccggcgc tgatgctggc gcgaccgtca ccatattgag
      361 caatttcagc tcatccggct tgaagacgaa cagtttatcg tgcggggtct ccatggcgtg
      421 gacccagacc tgcaggttca cgcccatgtc gccgagatag cgctggcagc gcgcggagat
      481 gttttgcgcc acgctcatct catcacgcag agggccgctg gcggccgaac tgatcgccgc
      541 cacctggtgc acgccgatta tggccttgtc ggcggcgcgt cgctcgacgc cgccggcgaa
      601 caccagcgga cacgacgacg cgcagtattt tcccgcctcg acttccgtcg agaatcttct
      661 ttcgcgaatg agccgcccca tcgtcagagc atcggccacc gagccgcccg gcgagttcag
      721 cgccacggtc ttgatataat cgccgcgcct gctgacttcc gccgcgaagg cttccgagat
      781 tcccggcgtg atagtgccgg tcgccatcaa cttgccgccg cccaccagtt cgaacgtcat
      841 cggcttggcc atcgcaccct gcggctgcag cagcggtagg aggcgcttgt cgccgccggg
      901 caacaatggt gccaggatcg acggcaccag gctgggaaga tccagcgtcg gtgaaccctg
      961 ctttagctcg gctggtgtcg ccgcggcgtc ggggttggcg atccacccgt tcatgctgat
     1021 gaggtcgagc gcgagcacgg cgattgtgac cgtgacgacg ctgcggaaaa tccagcgcaa
     1081 aatcgcttgg tccggattgt ccgacaacca ggcgtgaaaa cgttgctgca tggtgaggaa
     1141 ttgcggcacg gcgatccgct atttggtgag ccgcgccgac gacagcgagg tgacattttc
     1201 gtccgcggcc gccgccggct cggcaacggg ttggccgtct gtcttcactt cggccggcga
     1261 aacctcctcg tttggacttc ggcgccgcgc gtcctcgacc tcgcgcgcca tctgcaatgc
     1321 cgcgagcagc tcgcccgctg tcatgttgga tgcgctgaca gcgggaagcc cttcgcgccg
     1381 gatcgcggcg tgcacgacgc agaggataag caccagcacc gccggcatca ggtcgatcga
     1441 gatcgcgcca gcccaactcg ggatgaaatc gccggcatag cgcagcaccg cttcggcggg
     1501 cgaaagtgtc tgaaacctga tcggttcgat ccgcggcgtg gccaggatct tatccgctgc
     1561 gtccgaaagc gatgccgcct gtgcggcaat cgagctctcc accttgccaa ccaccgcggt
     1621 ctgccgatcc gcaagatcgg acgtgcgacc gcccgcggcc ggagcgatga aacccgatga
     1681 cagcgaggtt gcggcgcgct tgaccgccgg tgcgacggaa gtctgctgca aattggcgat
     1741 cacgcccatc agggccagcg actcggttgc gaatgcatcg ctgcgcgtcg aaatcggtcc
     1801 gcggtcgctg atcaattcgc gcatcctggc gagactcttg ccgccttgct cgtacaatgc
     1861 cgagacgcgt ttgcccgagg cctgaacttc ctggccgaga ctgtcgagct gcccggacat
     1921 ctgtgtcaga agctgcacca cggtgccgga tccggcggtg cccgtcagcg aaccggcacg
     1981 ctcggcgtcg gcaagtttcg cgaagcgcga tgacgccagt tggatatccg gcaacagtcc
     2041 ttgtgctgcg atggcgttgg tgttggcggt atcgagatcg cgggtgtagt tctgcacggt
     2101 gatggcaaga tgctgctgga tcgcagcggc cccggccagt gcggaggcgt tcagccatga
     2161 cgacatcgcc acgatcatca acgagccgag cagcatgcaa ccgaacatca ggctgcggcc
     2221 ggtgcgatcg atcacgtgcg gcatgaactg catcaggaat acccagaacg cgtagatgcc
     2281 gaccgatacc gcaaccgaat agatcaccgc ggcgaagaat accgtggtcg ggctgccgtt
     2341 gagcaattcg cggacgccga gaaaggtgta gacgccggcc gccagcgcga ggatcgcggt
     2401 ggtgagtttc agcatgcctt cgaggcgcgt gacgccgttg gacagcagcg ctgaagcaga
     2461 gcgggaaggc ggggcctgag atgccagggt gttattcggg ggcataatgg gttccgtgca
     2521 gttcaaaaaa caactgaaca gcgcccccgc tgctcgctaa acccttagct tctggagata
     2581 aattcggacg agattatgtc ggcaaccgtg gaaaacaaac gccaaatcgg ctatcatggc
     2641 gcatggcgac aaacaacgtt ttgaccgatg tgatcgcgga gttcggccgg aaatacctgt
     2701 ggtgggaacc tgtgagcgga cagccgcatt ccgaagatcg gatcatcacc caaacgatga
     2761 atctcgggac atacgacgac attcttctgc tggaggaggc ggtcggcaag gcgcgcttgc
     2821 ttgaagtaat gttgcgagcc gagcccggtt ggatcaacga ccgctcctgg gaattctggc
     2881 gcggccggtt gtcgtttgcc acgggagcgt cgatcccaga aaagggtccg cgaagagcgt
     2941 ttcatgccgc gacaccttga accgaaactc tccatcctgc cagcggcgca acggcaaatc
     3001 tggaccagcc tagcacccgc ccttcaattg agttttgtcc tctacggggg gacagccgtc
     3061 gcgctccatc tcggacatcg agaatcgctc gatttcgact ttttccgata tgaaccgctc
     3121 gacaaggatc aggtacgcga cgcgttcgga tttgttcgcg gggcagcaat cctgcaagac
     3181 tcgccggaca cgttggtggt gctggccgaa atgccatccg gcctggtgaa ggtttcgttt
     3241 ttcggtggca tcggctttgg ctgcgtcaat gatccacttc agacccgcga cggcacattg
     3301 cttgtcgcat ctctcgacga tctgatggcg accaagctga aggcgacact cgatcgcgcc
     3361 gaagccaaag attatcggga tatcgccgag atgatttccg cgggcgtgtc cttgccggct
     3421 ggcttgagcg cattcaagca aatgttcaat ggcgaaccgg cgcaagtcct gcgcgccctc
     3481 gggtatttcg aggatggaga tttggctacg ctcgataagg ccgatcagaa tcttctacgg
     3541 aatgcgcggg ataaaatcca acggctgcca agggtaattg tgaagtctgg ctctctcaca
     3601 ggacgttgat tcgagttgcc gacgtcaacc gcccagcgga cccattcgct ccacgagaaa
     3661 atcgatgaac agccgcgccc gcaccgggag ctgcctgccg aaggcgtgaa cgacatgcat
     3721 cggaaccttg ggcatcgcca gatcgggtag aatttccaca aggcgcccgg cggcgagatc
     3781 gtcttcggcg gcaaaccgca ggacctgggc gatgccggcg ccgtgaaggg ctgcgtgaaa
     3841 tagcgtgatg ccgtcgtccg aatcgagcgg tccatccggt gtcaacgagg tgccgtcggc
     3901 gaacatgaag gggtaggatc ttcctgccag cagatatcgc aggcaggcgt gtccacgcag
     3961 atcttcgatt gcacggggca cgccacggcg cgccaggtaa tcgggcgagg cgacaatcac
     4021 cattgaaagg ttggccagtt tgcgggccgt cagctccgtg tcctggagtt cgccgatgcg
     4081 cagcgcgaca tcgaaacctt cgcggatcag atcgacctgc cggtcggtga cgctgagttc
     4141 gagtttgacg ttcggatgtc gtgcgaggaa ggcctccgac catgccccga tcagcgtcct
     4201 cccaacaaag acaggggcgg tcacgcgcag caggccgcgg gcgttatcgg ccatctgcac
     4261 gacgtcctcg gcatccgcga tcgcgcgcag cagcggtgcg acgcgctcgt aatatgcggc
     4321 gccctcggct gtgaggctca gcgtccgcgt ggatcgctgc agcagccgca cgccgagccg
     4381 gcgctccagg cgcgcgacgc tctttgacac cgcagaagga ctggagccga tgaagcgccc
     4441 ggcgccggag aacgagcctg aatcgaccgt ctgcacgaag gcgaccagac cggcggtttt
     4501 ttcaagaatc gtggtcattc gagaattcca ggcacaattg atgtgctgag gatgcgccta
     4561 atcggctatc cgttcctgag tagataggtc gcagcagaca tcggcgatag agccggcaat
     4621 caggagaatt cggatgagtg acctcaaggg caaggtggcg atagtcaccg gcgcgtcgaa
     4681 aggtatcggc gcgggcatag cgaagggctt tgccgcggcg ggggccgcgg ttgtggtgaa
     4741 ttatgcctcc agccgggaag gcgcggaccg cgtcgtggct gatatcacag cccagggcgg
     4801 cagggcgatc gccgtgcaag gcgacgtgtc gaaggctgcc gacgtgaagc ggctgttcga
     4861 acaaaccaag gtgtcattcg gtgcgctgga cgtgctcgtc aacaacgccg gcgtctatca
     4921 gttcgacccg ctcgagactg tgacggaaaa ggaatttcac cgcgagttcg acaccaacgt
     4981 gctcggaacc atcctcacga tccaggaggc gctgaaatat ttcgggccgc atggcggcag
     5041 cgttatcaac atctcatcga tcgcaagcct gaacccgacg ccggactcgg tcgtctatgc
     5101 cgcaaccaag agcgcggtcg actcgatcac gcgcggcctg tcgaaggaac tgggcgcgcg
     5161 caaaattcgc gtcaacgcca tcgcgcccgg aggtaccgcg tcggagggac ttgtcacggc
     5221 cggcatcctc ggcagcgatt tcgaaaagca gattatcggc atgacaccgc tgggccgttt
     5281 cggccagccc gaagacatcg cgcgcgtggc cgttttcctg gcatccgacg actcctcgtg
     5341 gctgaccggc gaacgcatca ccgcgtccgg cggttggcgc tagagcggga tccggaaaag
     5401 tggggaccgg ttttccctcg ggacaaacgc gaggcgtttg cccggagatc atgctcaaac
     5461 aaaaagatag agcgggatga cgattcgaag aaaagtcgtc ccgctctagg ccgacgccgc
     5521 ggctgctgcg gagggctgta agcctcgcca gaagtgacta tctttctccc ctcgaaaggc
     5581 gttgccgcgg atg
//
LOCUS       DATNOI010000252         4011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_632160,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000252 DATNOI010000000
VERSION     DATNOI010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632160"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1894
                     /locus_tag="VN968_09795"
     CDS             <1..1894
                     /locus_tag="VN968_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXL29564.1"
                     /translation="GLPFFALAANNPLLQAWFVRTGHPSGPDPYFLYASSNIGSFLAL
                     LSYPVLLEPMLTLRTQNLIWTGGYALLIVLIGACGVLLLRSPATPAVDVLADETDAPA
                     PPWSLRARWIFLAAVPSGLLIAVTAHISTDIAAAPLLWVLPLSLYLLTWVLVFQSRPL
                     LPHQLMLRLQPLAITGVIVLLAVGGEQNLLLTLGGHQLCFFVIAMACHGELARTRPAA
                     NYLTGFYVALSFGGMIGGLFAGLIAPYTFSWIAEYPILLALAALCRPSGGNERFPRWS
                     SWYWPFLAVLAIALIAPSWSGGKVATWLGEHRVWVAGAVGVLSASLALGLNANRWKIF
                     ATVVVALVLIRAYPSDKGRVDTVRSFFGVHKILVSPRGQYHVLMHGTTIHGAEKFQNN
                     DGTPVTGRPEPITYYHEDGGIGQAIDAVRERKGAPLRVAVIGLGAGTLACASKPGESW
                     KFFEIDQTMVDTARDPKYFTYIQGCEPDLKPVIGDARLTFAREPDGIYDLIVVDAYSS
                     DAIPVHLATQQAMAIYKSKLAPQGAVVVHVSNRHLELSSVIVGIAETNDLKSWVYSED
                     SGRDDDYIFATSVVVCAREEADVGKLASSEDWALEEADENQRVWTDDYSNVLGAVWRR
                     LRDGEP"
     gene            2066..2293
                     /locus_tag="VN968_09800"
     CDS             2066..2293
                     /locus_tag="VN968_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HXL29565.1"
                     /translation="MPTSAEMKDHVYKILELVGSSEKSIDDAIQNAVTRASKTIRDMK
                     WFEVVQTRGHIEKGSVAHYQVTLRVGFTLER"
     gene            2458..>4011
                     /locus_tag="VN968_09805"
     CDS             2458..>4011
                     /locus_tag="VN968_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXL29566.1"
                     /translation="MHDQVDVLIIGSGASGAAVAWSLADTRMRILCLEQGDWVKPTDY
                     PSNGRDWEARLFGDFAVSPNRRARPTDYPINDANSPIKVVNFNGVGGSTIMYTAHFPR
                     LHPADFKVRTLDGVADDWPVDYATLEPFFAENDRMMGVSGLAGDPAYPPKQPPMPPLP
                     LGKSGTRFGKAMNQLGWHWWPSDSTIATTDYEGRARCINLGHCTPGCAQGAKASTDIT
                     YWPHAIRAGVELRTLCRVREITLDEHGMASGVVYYDAEGKEHFQPAEVVILACNGVGT
                     PRLMLNSVSARFPNGIANSSGLVGKNLMFHPYALTYGYVDEPLDGNHGPPLCLWSQEF
                     YETDPRRDFVRGYTFQFSRGVGAIFEAIVSSQAGRLPWGEDHHRVYRKLLNHRIGLSA
                     ICEDLPEEHNRVRLDPVLKDSSGIPAPRIDYTISANSLKMMEHGIARAKEILAAAGAG
                     NICVESPILNGGWHLLGTARMGSDPERSVVNEWGRCHDVKNLFIVDGSIWVTSGGVNP
                     TSTIQALALY"
BASE COUNT          707 a         1285 c         1271 g          748 t
ORIGIN      
        1 cgggctgccg ttcttcgcgc tggcggccaa caatccgctg ctgcaggcct ggttcgtccg
       61 caccggccat cccagcggtc ccgaccctta cttcctctat gcctcgtcga atatcggcag
      121 ttttctggcg ctgttgtcct atccggtgct gctggagccg atgctcacgc tgcgcacgca
      181 aaacctgatc tggaccggcg gctacgcgct gctgatcgtg ctgatcgggg cttgcggggt
      241 gctgctgctg cgctcgcctg caaccccggc cgtcgatgtg ctggccgatg agaccgacgc
      301 gccggcgccg ccgtggagcc tgcgcgcgcg gtggatcttt cttgccgcgg tgccatcggg
      361 cctcttgatc gcggtgaccg cgcatatctc caccgatatc gccgcggcgc cgctgttgtg
      421 ggtgctgccg ctatcgctct atctcctgac ctgggtcctg gtgttccagt cgcgcccgct
      481 gctgccgcac caattgatgt tgaggctgca accgctggcg atcacgggcg tgatcgtgct
      541 gctcgcggtc ggcggcgaac agaatctgct attgacgctc ggcggccacc agctttgttt
      601 ctttgtcatt gcgatggctt gccatggcga gctggcgcgc acccgcccgg ccgcgaacta
      661 tctcaccggc ttctatgtcg cgctgtcatt cggcggcatg atcggcggat tgttcgcagg
      721 gctgatcgcg ccctatacgt tttcatggat cgcggaatat ccgatcctgc tggcactggc
      781 ggcgttgtgc cggccgagcg gcggcaacga gcgttttccg cgctggagca gctggtactg
      841 gccatttctg gccgtgctcg cgatagcact gatcgcgccg tcctggtcgg ggggcaaggt
      901 cgcgacatgg ctcggcgagc accgggtttg ggtggccggc gccgttggcg tgctctcggc
      961 gtcgctggcg cttgggttga acgccaatcg ctggaaaatc ttcgcaaccg tcgtggtggc
     1021 gctggtgctg atccgcgcct atccgtcgga caagggccgc gttgataccg tgcgcagctt
     1081 cttcggcgtc cacaagatcc tggtgagccc gcgcgggcag tatcacgtct tgatgcacgg
     1141 caccacgatc cacggcgccg agaaatttca gaacaacgac ggcacgccgg tcaccgggcg
     1201 gcccgagccg atcacctact atcacgagga cggcggcatc ggtcaggcga tcgatgcggt
     1261 gcgcgagcgc aagggcgcgc cgttgcgggt cgcggtgatc ggccttggcg ccggcacgct
     1321 ggcctgcgcc tcaaagcccg gcgaaagctg gaagttcttc gagatcgatc aaacgatggt
     1381 cgataccgcg cgcgatccaa aatactttac ctacattcaa ggctgcgaac cggatctgaa
     1441 gccggtgatc ggcgacgcgc ggctgacctt cgccagggag ccggacggca tctacgacct
     1501 gatcgtcgtc gatgcctatt cgtcggacgc gatcccggtc catctcgcca cccagcaggc
     1561 gatggcgatc tacaagagca agctcgcgcc gcagggcgcc gtggtcgtgc acgtctcgaa
     1621 ccggcatctg gaactgtcca gcgtgatcgt cggcatcgcc gagaccaacg atctcaagag
     1681 ctgggtctac agcgaggatt ccggccgtga cgacgactat atcttcgcaa cctcggtcgt
     1741 ggtctgcgca cgcgaggagg ccgacgtcgg caagctggcg tcgtctgagg attgggcgct
     1801 ggaggaagcc gacgagaacc agcgggtgtg gaccgacgat tattccaacg tgctcggcgc
     1861 ggtgtggcgg cggctgcgcg acggcgagcc gtagagcatt gccgcagccg gcgagcagac
     1921 gatggttgat gttgccgcgc agaaggagag gaacccgccg ggtccagagc gcgttcacaa
     1981 tggacttggt tcacgatcgg accaaacgcc gggccggggg agataagcct gaaattgtcc
     2041 actgacacca caacggaggt tcgccatgcc aaccagcgcc gaaatgaagg atcatgtcta
     2101 caagatcctg gaactcgtcg ggtcgtccga aaaaagcatc gacgatgcga ttcagaacgc
     2161 cgtcacccgc gcctcaaaaa ccattcgcga catgaaatgg ttcgaggtcg tgcaaaccag
     2221 aggccatatc gagaagggat ctgtcgccca ctaccaggtc acgctgcgcg ttggctttac
     2281 gctggaacgc tgactcccgg caaaacaccc ccgtccagcc catgtcccgc tgaccggggc
     2341 taggcgggct gtgcggccac ggcttctagc cgctccttga ccccacgcgg atgtagggca
     2401 ggatcacaca cattcgattc ctgacggcct tcgcaaccgc gcgagagaaa aatttcgatg
     2461 catgaccagg tcgatgtgct gattatcggc tcgggcgcat cgggcgccgc tgtcgcgtgg
     2521 agccttgccg ataccaggat gcggatcctc tgcctcgagc aaggcgactg ggtcaagccg
     2581 accgattatc caagcaacgg acgcgattgg gaggcgcgcc tgttcggcga tttcgccgtc
     2641 agtcccaatc gccgtgcacg gccgaccgac tatccgatca acgacgccaa ttcaccgatc
     2701 aaggttgtga acttcaacgg cgtcggcggc agcaccatca tgtacacggc gcatttcccg
     2761 cgcctgcacc ccgcggattt caaggtgcgc acgcttgacg gtgtcgccga cgactggccg
     2821 gtcgactatg caaccctgga gccgttcttc gctgagaacg atcgcatgat gggcgtctcc
     2881 ggtctcgccg gcgatccggc ctatccgccg aagcagccgc cgatgccgcc gctgccgctg
     2941 ggcaagtcgg gcacccgctt cggcaaggcg atgaaccagc tcggctggca ttggtggccg
     3001 tcggattcga ccatcgccac caccgattac gaggggcggg cgcgctgcat caacctcggt
     3061 cactgcacgc cgggctgcgc gcagggcgcc aaggccagta ccgacatcac ctattggccg
     3121 catgcgatcc gcgccggcgt tgagctgcgc acgctgtgcc gggtccgcga aatcacgctc
     3181 gacgagcacg gcatggcttc cggcgtcgtc tattacgacg cggaggggaa agagcatttt
     3241 cagcccgccg aagtcgtcat cctcgcctgc aacggcgtcg gcaccccgcg gctgatgctg
     3301 aattcggtat cggcgcgatt tccaaacggc atcgccaatt ccagcgggct ggtcggcaaa
     3361 aacctgatgt ttcaccccta cgccctgacc tacggctatg tggatgaacc gctcgacggc
     3421 aatcacggcc cgccgctatg tctgtggagc caggaattct acgagaccga tcctcgacgc
     3481 gatttcgtgc gcggctatac gtttcagttc agccgcggcg tcggcgcgat cttcgaagcg
     3541 atcgtcagtt ctcaagcggg gcggctgccc tggggcgaag accatcaccg cgtctatcgc
     3601 aagctgctga accatcgcat cggtctttcg gcgatctgcg aggacctccc cgaggagcat
     3661 aatcgcgtga ggctcgatcc tgtgctgaag gattcgagcg gcatccccgc accgaggatc
     3721 gattacacca tcagcgccaa cagtttgaag atgatggagc atggcatcgc acgggcgaaa
     3781 gaaatcctcg ccgccgccgg cgccggcaat atctgcgtcg agagcccgat cctgaatggc
     3841 ggctggcacc tgctcggcac cgcgcgcatg ggcagcgatc ccgagcgctc ggtggtcaat
     3901 gaatggggcc gttgccatga cgtaaaaaac ctgttcatcg tcgacggcag catctgggtg
     3961 acttccggcg gcgtgaatcc gacctcgacc atccaggcgc tggcgctcta c
//
LOCUS       DATNOI010000253         2823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1992504,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000253 DATNOI010000000
VERSION     DATNOI010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992504"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="VN968_09810"
     CDS             complement(<1..1613)
                     /locus_tag="VN968_09810"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HXL29567.1"
                     /translation="MNAPTAIPTPTPVPPYKHTPLFPLGPDTAPYKKITTEGVRVEQV
                     LGKDMLVVSREALRALSEAAFGDINHYLRPGHLQQLRNILDDKEASDNDKFVAFDFLK
                     NANIAAGGVLPMCQDTGTAIIMGKKGFRVLTDGDDEAALSEGARDAYLRRNLRYSQVA
                     PLSMYEEKNTANNMPAQCEIYAEGGSLDSDLAYKFMFMAKGGGSANKSFLFQATPSVL
                     TRDRMLAFLKEKVLTLGTAACPPYHLAIVIGGTSAELCMKTVKLASARYLDTLPTHGS
                     ADGNAFRDLEMEQEILKMTQSLGVGAQFGGKYFCHDVRVIRLPRHGASLPIGLGVSCS
                     ADRQVLGKITKDGVYLEELEHNPAQYLPAVEQALGGEVVKIDLDQPMKDVLTTLSRHP
                     IKTRLSLTGTMIVARDSAHAKLRERLEKGEPLPDYFKNHPVYYAGPAKTPDGYASGAF
                     GPTTAGRMDSFVDQFQAAGGSMVMVAKGNRAVAVREACKKHGGFYLGSIGGAAANLAE
                     HCIKKVEVVEYPELGMEAIWRIEVEDFPA"
     gene            1833..2147
                     /locus_tag="VN968_09815"
     CDS             1833..2147
                     /locus_tag="VN968_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor family
                     protein"
                     /protein_id="HXL29568.1"
                     /translation="MKTRLVVLLAAALALMIFVPTGANAVGVGKMCGGIGGILCDAGL
                     FCQKKTGACSIIDMSGTCAKIPRFCPKHIQPVCGCDGKTYNNDCERQQAMVSNNHDGK
                     CY"
     gene            2435..>2823
                     /locus_tag="VN968_09820"
     CDS             2435..>2823
                     /locus_tag="VN968_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29569.1"
                     /translation="MATLGASPARADRCDDLAAQLKSQIDGLSIGKTAANLIYLSHPQ
                     AKQITLGCASRNISNQLFARADSRKPKPAFLELVAGASAIIFTLPKDEMLKGATRCIK
                     RMGLFRGDDVKTRYRRLDMNCSRTKTDA"
BASE COUNT          551 a          894 c          852 g          526 t
ORIGIN      
        1 aaggccggaa aatcctcgac ctcgatgcgc cagatcgctt ccatgccgag ctcgggatat
       61 tccaccacct cgaccttctt gatgcagtgc tcggcgagat tggccgcggc gccgccgatc
      121 gagcccagat aaaatccgcc gtgcttcttg caggcctcgc gcaccgcgac cgcgcggttg
      181 ccttttgcga ccatcaccat cgatccgccg gcggcctgga actggtcgac aaaggaatcc
      241 atccgccccg cggtggtcgg accaaatgcg ccggaagcat aaccgtccgg ggtcttggcg
      301 ggcccggcgt aatacaccgg atgattttta aaatagtccg gcagcggttc gcccttctcc
      361 agccgctcgc gcaatttggc atgcgcggaa tcgcgcgcga cgatcatggt gccggtcagc
      421 gacagccggg tcttgattgg atgccttgaa agcgtcgtca gaacatcctt catcggctgg
      481 tcgagatcga tctttaccac ttcgccgccg agcgcctgct cgaccgcggg cagatattgc
      541 gccggattgt gttcgagctc ttcaagatag acgccgtcct ttgtgatctt gccgagcacc
      601 tggcggtccg ccgagcacga cacgcccaag ccaatcggca acgaggcgcc atggcgcggc
      661 aaccggatca cccgcacatc gtgacaaaaa tatttgccgc cgaattgcgc gccgacgccg
      721 agcgactgcg tcatcttgag gatttcctgc tccatctcga gatcgcggaa agcattgcca
      781 tcagccgagc catgcgtcgg caacgtgtca agatagcgtg cggaggccag ttttacggtt
      841 ttcatgcaga gctcggcgga ggtgccgccg atcacgatgg cgaggtgata gggcgggcag
      901 gccgcggtgc caagcgtcag caccttctcc ttcaaaaacg ccagcatgcg atcccgcgtc
      961 agcaccgagg gcgtggcctg gaacaagaag ctcttgttgg ccgacccgcc gcccttggcc
     1021 atgaacatga acttgtaggc caagtctgaa tctaaagagc cgccctcggc atagatctcg
     1081 cactgcgccg gcatgttgtt ggcggtgttt ttctcctcgt acatcgacag cggcgccacc
     1141 tgcgaatagc gcaagttacg gcgcagatag gcgtcgcgcg cgccctcgct caaggcggcc
     1201 tcgtcgtcgc catcggtgag cacgcgaaaa cccttcttac ccatgatgat ggcggtgccg
     1261 gtatcctgac acatcggcaa cacgccgccg gcggcgatgt tggcgttctt cagaaagtcg
     1321 aaggcgacga acttgtcgtt gtcgctggcc tccttgtcat cgagaatgtt gcgcagctgc
     1381 tggagatgcc cgggccgcaa ataatgattg atgtcgccga acgcggcctc cgacagcgcc
     1441 cgcagcgcct cgcgcgacac caccagcatg tccttgccca acacctgctc gacccgcacg
     1501 ccctcggtcg tgatcttctt gtacggcgcg gtgtccggcc caagcggaaa cagcggcgtg
     1561 tgcttgtagg gcggaacggg ggtgggcgtg ggaatggcgg tgggggcgtt catgacatgc
     1621 tcgcagtttt gggaaccgga ggcgcctctt cgaggcgggg cgattgaaac gttctaagca
     1681 cttttgggag aaaaggaagc gagcgccgtc cggacgagcg cggcgaaatc cggctgcgct
     1741 tgcaccctgt cttcgccgcc attgcgcatc gccaggcgcc gagataacct ctggttgcta
     1801 atccaaaaat caaacgttgc agggaggcga ccgtgaaaac aaggcttgtt gttttactcg
     1861 cggcagcgct ggcgctgatg atcttcgtcc ccaccggcgc gaacgcggtc ggcgtcggaa
     1921 agatgtgcgg cggcattggc ggcatcctgt gcgacgccgg cctgttttgc cagaagaaaa
     1981 ccggagcatg cagcatcatc gacatgtccg gcacctgcgc aaagattccg cggttttgcc
     2041 caaagcatat tcagccggtc tgcggctgcg atggcaagac gtacaacaat gattgcgagc
     2101 gccaacaggc catggtttcc aacaatcatg acggaaagtg ctactagacg ccaccacgag
     2161 gtagccgcgc gccccgtctc aggtccaagc ggcggcgctc tcttgatccc cggcaaatgt
     2221 cctgccgtaa tagccaatgc gatcacggca cgtattgacg cggtttgaca gaccttgcat
     2281 gaaccttccg cgcgtttcaa tggcgcgggg ggtatccatg acaaaacagc agcttgcagc
     2341 gccgatctca aggcttaggt cggcaggttt tgggtcggca ggttttgggt ccgcgcgcgt
     2401 cgccgccgcg ctgacgctgc tggccgccat tgccatggcg acactcgggg cctcgccggc
     2461 gcgggccgac cgctgcgacg atctcgcggc ccagctcaaa agccagatcg acggcctcag
     2521 catcggcaag accgccgcca atttgatcta tctgtcgcac ccgcaggcca agcaaatcac
     2581 gcttggctgc gccagccgca acatcagcaa ccagttgttc gccagagccg acagccgcaa
     2641 gccaaagccg gcgtttctcg agctggtcgc cggcgcttcc gccatcatct tcaccttgcc
     2701 gaaggacgag atgctgaaag gcgcgacgcg ctgcatcaag cggatgggcc tgttccgcgg
     2761 tgacgacgtc aagacccgct accgccgcct cgatatgaat tgcagccgca ccaaaaccga
     2821 cgc
//
LOCUS       DATNOI010000254         7609 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1638435,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000254 DATNOI010000000
VERSION     DATNOI010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638435"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /gene="thrS"
                     /locus_tag="VN968_09825"
     CDS             complement(<1..1398)
                     /gene="thrS"
                     /locus_tag="VN968_09825"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HXL29570.1"
                     /translation="MPDKHESASGFQYSITNLKPAEPTQKIAVTFPDGARREFPKGIT
                     GLDIAKGISPSLAKRTVAMGLDGELTDLADHIEHDVKIEFVARDDPRALEMIRHDAAH
                     VLAEAVQSLWPGTQVTIGPVIENGFYYDFFRNEPFTPDDFAAIEKRMREIIARDKPFT
                     KEIWTREQARQVFADNGEMFKVELVDAIPTDQTIKIYKQGDWFDLCRGPHMTSTGKIG
                     NAFKLMKVAGAYWRGDSNNPMLTRIYGTAFAKQEDLDAYLKQIEEAEKRDHRKLGREL
                     DLFHFQEEGPGVVFWHPKGWTVFQELIAYMRRRLAGDYSEVNAPQILDKALWETSGHW
                     DWYRENMFAAQSAGDDAEDKRWFVLKPMNCPGHVQIFKHGLKSYRDLPLQLAEFGVVH
                     RYEPSGAMHGLMRVRGFTQDDAHVFCTEAQLADECLKINDLILSTYADFGFEGDLTVK
                     LSTRPDKRVGTDEMWD"
     gene            1614..1868
                     /locus_tag="VN968_09830"
     CDS             1614..1868
                     /locus_tag="VN968_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29571.1"
                     /translation="MPVTPEILRTSKQLRDMLWPFDFVVVDNEEYGPVWFDTAPLEPF
                     EIVAQRSTGCVYALTGAQRHVLLATSEGSRASWRRACRNV"
     gene            1861..2211
                     /locus_tag="VN968_09835"
     CDS             1861..2211
                     /locus_tag="VN968_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29572.1"
                     /translation="MSELVIARPYWQDVLRYGKGDLSAMRDVLRDRIEEFEEEALDDN
                     PEIEEFRPLPAPTAGIGRAQRPRKTAASWGHGAWCQGGRSQFGRLSVRAAGRQIQEAI
                     IAACSNPSPGNEIT"
     gene            2257..3432
                     /locus_tag="VN968_09840"
     CDS             2257..3432
                     /locus_tag="VN968_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL29573.1"
                     /translation="MKSTNVERRFAATALMLGNFITGTSILAPAGMLGELSEGLQVSI
                     RDAGLLITFGAIVLCIASPLAAWLTSRIERHALLTATLAVLAIANAASAFAPDYATLL
                     VIRLIMLAVAALYTPQAAGTAALIVPVEKRGGTIAYIFLGWSLAAAIGLPLVTFIASR
                     YGWRATYGCIGVAACFSCLLLAWRLPGGLIGAPVDLKTWADLGRNRLIVLLLLITALQ
                     VSGQFAVFTYLGPLLTRLTRASPDAVGLAFATYGVFGFLGNVIATRLVDSWGSYRTSL
                     LFMALLLAGACIWALGAGNFTIMAAAVAVWGLGFASTNSMQQVRLVGAAPALGAASVS
                     LNTSALYIGQAIGSAAGGVLFARDLLHGAGYVSVAFIALALGVVTLTRPRPTVALST"
     gene            3518..3886
                     /locus_tag="VN968_09845"
     CDS             3518..3886
                     /locus_tag="VN968_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29574.1"
                     /translation="MRTIFAVVLALYSGAAMAQTSPPTVGGKPLVQVKPSGEAAKSKS
                     KATAKPKAPSTAAKVQACLEIDDATKERLNCYDGIFPPQPKPKAPTAKAVNDCRFHKE
                     EDERLTCFNGFAEKIPKLPQ"
     gene            complement(3929..5122)
                     /locus_tag="VN968_09850"
     CDS             complement(3929..5122)
                     /locus_tag="VN968_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL29575.1"
                     /translation="MRWIALLSALVPLSFVLIQCGRAPSPGTLAANAQAGAGDSFDDR
                     FPAPQFRDRFPSANESFVQRQAPDSSRKRTAQAEPAPYRVASLAPTVPYQRPAREDQT
                     ALIGLKSSAFPYLGNNPRTDEPFLNVAKGDRRGHRSYGGRVYWQDETYNDNRVLMHVP
                     EHFDVRKPGVIVVFFHGNGATLERDVRDRQLVPQQITDSGVNAVLLAPQLAVDAADSS
                     AGKFWQPGGLKRFVAESTDHLARLYGDPGAAKAFANMPVVIIGYSGGFLPAAWSLEVG
                     GLGNRVRGVFLLDAVYGELDKFASWIENNRSGFFVSAYTRYTKRHDRELMQILKDKGI
                     AISEDMDGPLRPGSAVFVETPEGVTHRDYVTHAWTENPVKEVLLKMAPALTRMAASAS
                     APASR"
     gene            5524..6897
                     /locus_tag="VN968_09855"
     CDS             5524..6897
                     /locus_tag="VN968_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXL29576.1"
                     /translation="MTTSETGSVAWTGQLPRSGSTVSMLVATAIVVADMVGVGVFTSL
                     GFQVKDIPSGFSILLLWAVGGIVALCGVFSYSELGAMFPRSSGEYNFLGRAFHPAFGF
                     VAGWVSATVGFAAPVALAAMAFGEYGKSVLPGVPPLALAIGIVWLVSIVQLTGVRHSS
                     TFQLISTVLKVVLIVAFLIAGFVIGTPQPVSFAPDVSDFAHVTSAPFAIGLVFVMYSF
                     SGWNAATYIIGEMRMPQQNLPRALLTGTLIVLVLYVALNAVFLHTAPIDKLSGQIDVA
                     RIAGSYIFGELGGRIVGAMICIGLVSSISAMMWIGPRVMMTMGEDIPSLRVFARKSTS
                     GAPAYAILFQLAVANLMLFTRSFEAVLDFIQFALLFCSFFTVLGVIKLRIAQPDLPRP
                     YRAWGYPITPAVFLLVTGFMMYYLLTERPLQSALGTLIMISGLLIYAVFRNRAVPGFP
                     AASPSRE"
     gene            6900..>7609
                     /locus_tag="VN968_09860"
     CDS             6900..>7609
                     /locus_tag="VN968_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29577.1"
                     /translation="MLQPVRIAAVAATMFLAAAAAARAADTVSADDTAHFLAGMAPSP
                     DSPLAPLTRDPAWQRHARFFDAAFAQLEQRQLSKIRGWAEINLAAPRPTMLYMFSGPD
                     FLYADAFYSKATTYVLSALEPVGSVPDLTKLPRGSIGSTLYHIERSLSSVLSFSFFIT
                     KLMKSDLHAGQVGGTLPILYVFLARSGKTIRDVSLISLDDKGAAYFANENAGPNATRG
                     VRILFAGSDGQERTLYYFS"
BASE COUNT         1307 a         2343 c         2387 g         1572 t
ORIGIN      
        1 atcccacatc tcgtcagtgc cgacgcgctt gtccggccgg gtcgagagct ttaccgtgag
       61 atcgccctcg aagccgaaat ccgcataggt cgacaggatg agatcgttga tcttcaggca
      121 ctcgtcggcg agctgggctt cggtgcagaa cacatgcgcg tcgtcctggg tgaagccgcg
      181 cacccgcatc aagccgtgca tcgcgcccga cggctcgtag cgatgcacca cgccgaattc
      241 ggccagttgt aacggtaggt cgcgatagct cttcaggcca tgcttgaaga tctgcacgtg
      301 gccgggacag ttcatcggct tcagcacaaa ccagcgcttg tcctcggcat cgtcgccggc
      361 cgattgcgcc gcaaacatgt tttcgcggta ccagtcccaa tggccggagg tttcccacag
      421 cgccttgtcg agaatctgcg gggcgttcac ttcgctgtaa tcgcccgcca ggcgccggcg
      481 catataggca atcagttcct ggaagacggt ccagcccttc gggtgccaga acaccacgcc
      541 ggggccctct tcctggaaat gaaacaggtc gagttcgcgc cccaatttgc ggtggtcgcg
      601 cttctcggct tcctcgatct gcttcaggta ggcgtcgagg tcttcctgct tcgcgaacgc
      661 cgtgccgtag atccgcgtca gcatcggatt gttggagtcg ccgcgccaat aggcgcccgc
      721 gaccttcatc agcttgaacg cattgccgat cttgccggtg gacgtcatgt gcggtccgcg
      781 gcacagatcg aaccagtcgc cctgcttgta tatcttgatg gtctggtcgg tcgggatcgc
      841 gtcgaccagt tcgaccttga acatctcgcc gttgtcggcg aatacctgcc tggcttgctc
      901 gcgggtccag atttccttgg tgaagggttt gtcgcgcgcg atgatctcac gcatccgttt
      961 ttcgattgcc gcaaaatcgt ccggcgtgaa cggctcgttg cggaagaaat cgtaatagaa
     1021 gccgttctcg atcaccgggc cgatcgtcac ctgggtgccc ggccacagcg actgcaccgc
     1081 ttcagccagc acatgcgcgg catcgtgccg gatcatttcc agcgcgcgcg ggtcgtcgcg
     1141 ggcgacgaat tcgatcttga cgtcatgctc tatatggtcg gcgagatcgg tgagttcccc
     1201 atcgagtccc atcgccaccg tgcgcttggc cagcgacggc gagatgcctt tggcgatatc
     1261 caggccagtg atgcctttgg ggaattcgcg cctggcgccg tcggggaagg tgacggcgat
     1321 cttttgcgtg ggctcggccg gtttcaggtt ggtgatgctg tactggaatc cagaggcgga
     1381 ttcgtgcttg tcgggcatag gtctctcctg tggctcactc ctgcgaacga gcgcaggtaa
     1441 gcgggaaaca gcggtatagc aggggattcg ggcggtgcaa tcggcaccct caagaaagtc
     1501 agcggagaac cggctcccgg attgaacggc cctccttccc gaagcaacca acgcggatat
     1561 gccatgcgcc ggagcgtcgc tcgcgcgctc gccatcacgt atgagctcag ccgatgccgg
     1621 tgacaccgga aattctcaga acgagcaaac agcttcggga catgttgtgg ccgttcgatt
     1681 tcgtggtggt cgataacgag gaatatggac cggtctggtt cgacaccgcg ccattggagc
     1741 cgttcgagat cgtggcgcag agaagcaccg gctgcgtgta cgcgttgaca ggtgcgcaac
     1801 gccacgtcct gctagccaca tccgaaggca gccgggcatc atggcggcga gcctgcagga
     1861 atgtctgagc tcgtgatcgc gcgcccttat tggcaggatg tcctgcgcta tggaaagggg
     1921 gacttgtcgg cgatgagaga cgtcctgcgc gacaggatcg aggaattcga ggaggaggcg
     1981 ctcgacgaca atccagaaat cgaagagttt cggccgctgc ctgcgcccac ggctgggatt
     2041 ggccgagccc agcgaccccg caaaactgct gcatcatggg gtcatggtgc ttggtgccaa
     2101 ggtggtcgtt cgcaattcgg acggttatcc gtcagagccg ctggccggca gattcaagaa
     2161 gcgataatcg cggcttgcag caatccttct cccggcaacg agatcacctg acgctcccgc
     2221 tttccttccg cgtggatatg atctacatgc atatgcatga aaagtaccaa tgtcgaacgc
     2281 cgcttcgctg ccacagcctt gatgcttgga aatttcatca ccggcacttc cattctggcg
     2341 ccggcaggca tgttgggcga actgtcggaa ggactgcaag tcagcatccg cgacgccggc
     2401 ctgctgatca cgttcggcgc gatcgtgctg tgcatcgcct cgccgctcgc ggcatggttg
     2461 accagccgga tcgaacgcca cgcgctgctc accgcgacgc tcgcagtgct ggcgatcgcc
     2521 aacgccgcgt cggcgttcgc gcctgactat gcgaccctgc tcgtcatccg cctgatcatg
     2581 ctggcggtcg ccgcgctcta tacgccgcag gccgcaggga ccgccgcgct gattgtgccc
     2641 gtggagaaac gcggcggcac catcgcctat atcttcctcg gatggtcgct cgccgcggcg
     2701 atcgggctgc cgctggtgac gttcattgcc agccgctacg gctggcgcgc gacctacggc
     2761 tgcatcggcg ttgccgcctg cttcagttgc ctgctgctag cctggcgtct gcccggcgga
     2821 ttgatcggcg caccggtgga cctcaagaca tgggccgatc tcgggcgaaa ccggctgatc
     2881 gttctgctgc tattgatcac ggccttgcag gtctcgggac agttcgccgt tttcacctat
     2941 ctgggcccgt tgctgacgcg gctgacccgg gcgagcccgg acgcggtcgg gctggcgttt
     3001 gcgacctatg gcgtgttcgg atttctgggc aacgtgatcg cgacccgcct ggtcgatagt
     3061 tggggcagct accggacatc gctattgttc atggccttgc tgctggccgg ggcctgcatc
     3121 tgggcgcttg gcgccggcaa tttcacgatc atggcggccg cggttgccgt ctgggggctt
     3181 ggttttgcct cgaccaattc catgcaacag gtacggctgg ttggcgccgc cccggcgctc
     3241 ggcgccgcgt cggtgtcgct gaacacttcg gcgctctata taggccaggc gatcggctcc
     3301 gcggccggcg gggtactgtt cgcgcgcgac ctgctccacg gcgcgggcta cgtgtcggtg
     3361 gccttcattg cgctggcgct tggcgtggtg accctgacca ggcctcgccc tactgtggcc
     3421 ttgtcaacat aagcgggacg ccttatccgc tcgaacccgt cgacaagatt ttcgccgcgt
     3481 gcttttcttc agggcgcggg aatctggagg gggtgaaatg agaacaatat ttgcggtcgt
     3541 cttggcgctg tattcgggcg cggcaatggc tcaaaccagc ccgccgacgg tcggaggaaa
     3601 gccgctggtc caggtgaagc ccagcggcga agctgccaaa tcgaaatcga aagcaacagc
     3661 gaaaccgaaa gccccgtcaa ccgccgcgaa ggtgcaggct tgtctcgaaa tcgacgacgc
     3721 aacgaaagag cggttgaatt gctacgatgg cattttcccg ccgcagccaa aacccaaggc
     3781 gcccacggca aaagccgtca atgattgccg gttccacaag gaagaagacg agcggctgac
     3841 atgctttaac gggttcgccg agaaaatacc caagctcccg cagtaatccg tcgccgctcg
     3901 gttttttccc gagggacggc gtaccggctt atcggcttgc gggcgcagag gcgcttgccg
     3961 ccattctcgt cagcgcaggc gccatcttga ggagcacctc cttgaccggg ttttcggtcc
     4021 aggcgtgggt gacgtaatca cgatgcgtca cgccttcggg ggtctcgaca aataccgcgc
     4081 tgccgggccg caacggcccg tccatgtcct ctgagatcgc gatgcctttg tctttcagga
     4141 tttgcatgag ctcacgatcg tgccgcttgg tgtagcgcgt atacgcgctg acgaaaaagc
     4201 cggatcggtt attctcgatc caggaggcga acttgtccag ttcgccatag accgcatcga
     4261 gcaggaacac accgcgtacc cgattgccaa gtccgccgac ttccaggctc catgctgccg
     4321 gcaggaagcc gccgctgtag ccgatgatga cgaccggcat gttcgcgaaa gccttggcgg
     4381 caccggggtc gccgtagaga cgggcgaggt gatcggttga ctccgccacg aagcgcttga
     4441 gcccgccggg ctgccagaac ttgccggcgc tggaatcggc ggcatcgacc gcgagctgcg
     4501 gcgccagcag cacggcattg acgccggaat cggtaatctg ttggggcacg agttgccggt
     4561 cacgcacgtc gcgctcaagt gtcgcgccgt tgccgtggaa gaaaaccacg atgacgcccg
     4621 gtttgcggac atcgaaatgc tcggggacgt gcatcaggac gcggttgtcg ttgtaggtct
     4681 cgtcctgcca gtaaacccgg ccgccatagc tgcgatggcc ccggcggtcg ccctttgcga
     4741 cgttcaggaa cggctcgtcc gtgcgcggat tgttgccgag atacggaaac gccgaggatt
     4801 tcaggccgat cagcgcggtc tgatcttctc tggccggacg ctggtatgga accgtcggtg
     4861 ctaacgaggc gacccggtat ggggcgggct ccgcctgcgc cgtccgtttg cgggacgaat
     4921 cgggagcctg ccgctgcaca aaactctcat ttgcgctcgg gaagcgatcc ctgaactgcg
     4981 gcgccgggaa gcgatcgtcg aaggagtccc cggctccggc ctgcgcattg gctgcgagcg
     5041 tgccggggct cggcgcccgt ccgcactgaa tgagaacgaa cgacaacggc accaacgccg
     5101 acaagagggc gatccagcgc aatcgatgag agccggcgcg gccttgggaa gcttccggcc
     5161 gcgcatgccg cggaacgccg ggtgacgtca cctggcacgc gcgatcatat gtctggaatc
     5221 tcgccccgat catccaccca ctgccccaat gcccaccggc ggccgtcgta tatcagcgcg
     5281 ccagtgttga ggtgacgtta agctttcggg aaaattctcc ccacttcccg aaggcctcag
     5341 aaaacacgaa atcgtgtcgc gattgtgggg cggcaagcgt gttttcgcaa tgccgcaggc
     5401 aagccggccg atcaaaggta ttgaaatacc taatcgtggt cgggagttaa accattgtta
     5461 agactacttg aattgatcgg ggcggggcgg cacgatgaaa aaactggggg cgctgggacc
     5521 ggcatgacga catcagaaac gggaagcgtg gcgtggactg gccagctccc gagaagcggg
     5581 tccacggtct cgatgctggt tgccaccgcc atcgtcgtgg ccgacatggt cggcgtcggc
     5641 gtcttcacta gccttggctt tcaggtcaag gatattccat cggggttttc gatcctgttg
     5701 ttatgggccg ttggcggcat cgtcgcgctg tgcggggtgt tttcctacag cgaactgggg
     5761 gcgatgtttc cgcgctcgag cggcgaatac aattttttgg gccgcgcctt tcatccggct
     5821 ttcggtttcg tggcgggctg ggtgtcggca accgttggct tcgcagcacc ggtggctctg
     5881 gccgcgatgg ctttcgggga atacggcaaa tcggtgcttc cgggcgttcc gccgctggcg
     5941 cttgcgatcg gcatcgtctg gctggtgtcg atcgtgcaac ttacaggcgt caggcactcc
     6001 agcacctttc agctcatctc gaccgtgctg aaagtcgtgc tgatcgtcgc cttcctgatc
     6061 gcgggattcg tgattggcac gccgcaaccg gtgtcgtttg cgcctgatgt ctccgacttc
     6121 gcccatgtca ccagcgcgcc gttcgcgatc ggtctggttt ttgtgatgta ttcgttttca
     6181 ggttggaatg ctgcaaccta catcatcggc gaaatgcgca tgccgcagca gaacttgccg
     6241 cgcgcgctgc tcacggggac gctgatcgtt ctcgtgctct atgttgccct caacgcggtt
     6301 ttcctccaca ccgcgccgat cgacaaattg tccggtcaaa tcgacgtcgc ccggatcgcc
     6361 ggcagctaca tcttcggcga gctcggcggc cgcatcgtgg gcgccatgat ctgtatcggg
     6421 ctggtgtcct cgatcagcgc gatgatgtgg atcggcccac gcgtcatgat gaccatggga
     6481 gaggacatcc cgtcgttgcg ggtgttcgcc cgcaaatcga ccagcggagc gccggcgtat
     6541 gccatccttt tccagcttgc ggtcgcgaac ctcatgctgt tcacccgcag tttcgaggcc
     6601 gtgctcgatt tcatccagtt cgcgctgttg ttctgttcgt tcttcaccgt gctcggagtc
     6661 atcaagctgc gcatcgccca gcccgatctg ccgcggccct accgtgcctg gggctatccg
     6721 ataacgccgg cggtcttcct gctcgtgaca ggtttcatga tgtactacct tttgacggaa
     6781 cggccgttgc agtcggctct cggtacgttg atcatgattt cgggccttct gatctacgcc
     6841 gttttccgca accgggccgt cccaggcttc cccgccgcat ccccaagccg cgaatgaaga
     6901 tgcttcagcc ggtgcgaatt gcagccgttg ctgcaaccat gttcctcgcg gccgcagctg
     6961 ctgcgcgcgc cgccgacacg gtgagcgccg acgacaccgc gcatttcctt gcggggatgg
     7021 cgccatcgcc ggattcgccg ctggcgccgc tgaccaggga tccggcctgg cagcgccacg
     7081 ccaggttctt cgatgcggcg ttcgcccagc tcgagcagcg ccagctttcg aaaatccgcg
     7141 gctgggcgga gatcaatctg gcggcgccgc ggccgacgat gctctacatg ttcagcggtc
     7201 cggatttcct ttacgcggac gcgttttatt ccaaggccac gacctacgta ttgagcgcgc
     7261 tcgagccggt gggctcggta cccgatctca ccaaattgcc gcggggtagt atcggatcga
     7321 cgctgtacca tatcgagcgt tcgctgagct cggtcctgag cttcagcttt ttcatcacca
     7381 agctgatgaa gtcagatctg cacgccggac aggtcggcgg caccttgccg atcctttatg
     7441 tgtttctggc gcgctcgggg aagaccatcc gcgacgtcag cttgatctcg ctcgacgaca
     7501 agggcgctgc gtatttcgcc aacgaaaatg ccggcccgaa cgcgacgcgc ggcgtccgca
     7561 tcctgttcgc cggaagcgac ggccaggaaa ggacgctgta ttatttctc
//
LOCUS       DATNOI010000255         3415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2013465,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000255 DATNOI010000000
VERSION     DATNOI010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013465"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VN968_09865"
     CDS             <1..218
                     /locus_tag="VN968_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL29578.1"
                     /translation="PLEVKEKMFNPFFTTKPAGEGTGLGLSMSHDIVVKQHGGKIDVD
                     TMPGVFTEFIITLPRTAAAQAQTGVSN"
     gene            215..604
                     /locus_tag="VN968_09870"
     CDS             215..604
                     /locus_tag="VN968_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL29579.1"
                     /translation="MSVYILVVDDEPDVEALFRQQFRRDLRSGRFTMEFVPSAPAALA
                     RAANVADPSLILILSDINMPGMSGLEMLPKVRAERPDVPVIMITAYGDVETRRKAIER
                     GAVGLLTKPIDFALLRHEIDTRLGQAA"
     gene            601..1851
                     /locus_tag="VN968_09875"
     CDS             601..1851
                     /locus_tag="VN968_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL29580.1"
                     /translation="MTATILVVDDEPDLEALILQKFRRQIRDGEVAFVFARDGIEALQ
                     SIQDHPHVDMVVSDINMPRMDGLSLLLKLQEAEDKKSTIIVSAYGDMSNIRTAMNRGA
                     FDFLTKPIDFGDLETTIDKTIRHVEMMREARRRQAEAERAHASLSRYFSPQIAARLAA
                     VGESNGMEVHWREVATIFTDITGFTSLVETAAPEVLGALLNEYVGGMTDVVFAHEGTV
                     AKIIGDAVQILFNAPGDQPDYATRAIACAHDLDVWAEAFRGRWKSKGVNFGATRIGVH
                     AGPALVGNFGGGRFFDYTAYGDTINTAARLEGANKFLGTRICVSASVADGTKKFRGRP
                     VGDLMLRGRSEPLRAYEPLTAETFKRPVTRQYSEAFAKLEAGDASAMPAFAALVGLHA
                     DDALAGFHLRRLLNGAKGVRMQLE"
     gene            1882..2625
                     /locus_tag="VN968_09880"
     CDS             1882..2625
                     /locus_tag="VN968_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29581.1"
                     /translation="MTRDFPAGNYRFIPAVFQYSSGAAAGSGFEVERVRFDRLLPLAE
                     GFASAAKYIQAAGQPLTSLCACELRSPAAFTEDGFRTFNEHYVKTLAQWGLFDGSVNP
                     VARSNVCPEIDPPAEPSFYAFSFTRPSQGQSPTFVIAGGAEARGGPGSYPERIVRYRD
                     LSPDGVREKVRFTVGEMEDRLDAFGFGWRDTTAVQAYTVRDFHPLMSDELVRRGATRC
                     GLTWHFARPPVIDLEYEMDCRRVMRETVI"
     gene            2681..2872
                     /locus_tag="VN968_09885"
     CDS             2681..2872
                     /locus_tag="VN968_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29582.1"
                     /translation="MPVKIFYAQGNKNIAGLEAEINKWQESLGPRASVTHTSTAMAEH
                     RDDVNGGETRVIVTIWFEL"
     gene            complement(2909..>3415)
                     /locus_tag="VN968_09890"
     CDS             complement(2909..>3415)
                     /locus_tag="VN968_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL29583.1"
                     /translation="ALISRITDAMLACPFLILAIALAAFLGPSLGNAMIAIGISTTPI
                     FVRLTRGQVMTVKVEDYVEAARAMGNPRWRIALFHILPNIMPALLVQATLSIAAAIIA
                     EAALSFLGLGQQPPAPSWGSMLNAAQRFLTNAPWMALWPGLAIFLVVLSFNLVGDGLR
                     DALDPRQR"
BASE COUNT          670 a         1062 c         1076 g          607 t
ORIGIN      
        1 tccccttgga agtgaaggag aaaatgttca atcccttctt caccaccaag cccgctggcg
       61 agggcaccgg acttggcctg tcgatgagcc acgacatcgt ggtgaaacaa cacggcggca
      121 agatcgacgt cgatacgatg cccggcgtat ttactgaatt cattattacg ctgccgcgga
      181 cggcggcagc acaagcgcaa actggagttt caaattgagc gtttacatcc tcgtcgtcga
      241 tgacgagccc gatgtcgaag ccttgttccg tcagcagttt cggcgcgacc tgcgctccgg
      301 acgcttcacg atggaattcg tcccgtcggc gccggcagca cttgcccgcg ccgcgaacgt
      361 tgccgaccct tcgctgatcc tgatcttgtc cgacatcaac atgcccggca tgagcgggct
      421 cgagatgctg ccgaaggtgc gtgcggagcg gcccgacgtt cccgtcatca tgatcaccgc
      481 gtatggcgat gtcgaaaccc ggcgcaaggc gatcgaacgt ggcgcggtcg gccttttgac
      541 caagccgatc gatttcgcgc tgctccgcca cgaaatcgat accaggcttg ggcaagccgc
      601 atgaccgcga ccatcctcgt cgtcgatgac gagcccgatc tcgaggctct gatcctgcaa
      661 aagttccgca ggcaaattcg cgacggcgag gtggcgtttg tattcgcccg cgacggcatc
      721 gaggcgctgc agtcgatcca ggatcatccg catgtcgaca tggtggtgtc cgatatcaac
      781 atgccgcgga tggacggcct ttccctgctg ctgaagctgc aggaggccga ggacaagaaa
      841 tcgaccatca tcgtttccgc ctatggcgac atgagcaaca tccgcaccgc gatgaatcgc
      901 ggcgcgttcg atttcctgac caagccgatc gatttcggcg acctcgaaac caccatcgac
      961 aagaccatcc gtcatgtcga gatgatgcgc gaggcgcgcc gccgccaggc cgaggccgag
     1021 cgcgcgcacg cgtcgctgtc gcggtatttt tcgccgcaga tcgccgccag gcttgccgcc
     1081 gtcggcgaaa gcaacggcat ggaggtgcat tggcgcgagg tcgcgaccat cttcaccgac
     1141 atcaccggtt tcacgtcgtt ggtggaaacc gccgcacccg aagtgctggg tgcgctgctc
     1201 aacgaatatg tcggcggcat gaccgatgtc gtgttcgcgc atgaggggac ggtcgccaag
     1261 atcatcggcg acgcggttca gatactcttc aacgcgcccg gagaccagcc ggattatgcg
     1321 acgcgcgcca tcgcctgcgc gcatgacctc gacgtctggg ccgaggcgtt tcgcggacgc
     1381 tggaaatcca aaggcgtgaa tttcggggca acccggatcg gcgtccacgc cggcccggcg
     1441 ctagtcggca atttcggcgg cggccgcttt ttcgattaca ccgcttatgg cgacaccatc
     1501 aacaccgcgg caaggctcga gggcgccaac aagtttttgg gtacccgcat ttgcgtcagc
     1561 gccagcgtgg ccgatggcac caaaaagttt cggggacggc cggtcggcga tttgatgctg
     1621 cgcgggcgca gcgagccgct gcgcgcctac gagccgctga cagcggagac gttcaagcga
     1681 ccagtcacca ggcaatactc cgaagccttc gccaagctgg aagccggcga tgcctcggca
     1741 atgccggcct tcgctgccct ggtcgggctg catgccgacg atgcgctggc cggctttcac
     1801 ctcagacggc tgctcaacgg cgccaagggc gtccgcatgc agctggaata aacatccaag
     1861 tgacgttagg ggacaccaga catgacgcgt gactttccgg ccggcaatta tcggttcatc
     1921 ccggcggtgt ttcaatattc gagcggggct gcggctggtt caggctttga ggtcgagcgc
     1981 gtgcgcttcg atcgcctgct gccgctggcc gagggttttg cgtctgccgc gaaatatatc
     2041 caggcggcgg gccagccgct gacgtcgttg tgtgcctgcg agctgcgctc gccggcggcc
     2101 ttcactgaag atggcttccg aaccttcaac gagcattacg taaaaacgct ggcgcaatgg
     2161 ggcttgttcg acgggtcagt caatccggtg gcgcgcagca atgtctgtcc ggagatcgac
     2221 ccgccggccg agccgtcgtt ctacgcgttt tcgttcaccc gtccgagcca aggccaatca
     2281 ccgacctttg tgatcgccgg cggcgccgaa gcgcgcggcg gccccggcag ctaccccgag
     2341 cgtatcgtgc gctatcgcga cctcagccca gacggcgtga gggagaaagt ccggttcacg
     2401 gtcggagaaa tggaagaccg tctcgacgcg ttcgggttcg gctggaggga taccacggcg
     2461 gtgcaggcct acaccgttcg ggactttcat ccgctgatgt cagatgaact ggtccgccgc
     2521 ggcgccacgc gttgtggcct gacctggcat tttgcgcgcc cgccggtgat cgacctcgaa
     2581 tacgagatgg attgccgccg cgtcatgcgc gagacggtga tctagaatat ttcccgcgcg
     2641 cgccgcacga ctaaaatcga accgcaaggg agagctgcaa atgccagtca agatctttta
     2701 cgcgcagggc aacaagaata tcgcgggatt ggaggctgaa atcaacaaat ggcaggaaag
     2761 ccttggcccg agagcctccg tgacccatac atcgacggcc atggcagagc atagggacga
     2821 cgtaaacggc ggggagacgc gcgtgattgt gacgatctgg ttcgaattat gatcggccga
     2881 aactcagacc aggaaaccat tggttcgctc agcgctggcg cggatcgagc gcgtcgcgca
     2941 ggccgtcgcc gaccagattg aacgacagca ccacgagaaa gatcgcaagc cccggccaca
     3001 gcgccatcca cggcgcgttg gtcagaaagc gttgcgcggc attgagcatg ctgccccagg
     3061 acggcgcggg cggctgctgg cccaagccta agaacgacag cgccgcttcg gcgatgatcg
     3121 cagccgcaat cgacagcgtt gcctgcacca gcagcgccgg catgatgttc ggcagaatat
     3181 gaaacagcgc gatccgccag cgcggattgc ccatcgcccg ggcggcctcg acatagtcct
     3241 cgaccttgac ggtcatcacc tggccgcggg tcaaccgcac gaagatcggc gtcgttgaaa
     3301 tgccgatggc gatcatggcg ttgccgaggc tcgggcccag aaacgccgcc agcgcgatcg
     3361 caaggatcag gaacgggcag gccagcatgg cgtcggtgat gcggctgatc agggc
//
LOCUS       DATNOI010000256         5191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1094285,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000256 DATNOI010000000
VERSION     DATNOI010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094285"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="VN968_09895"
     CDS             <1..1172
                     /locus_tag="VN968_09895"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HXL29584.1"
                     /translation="VPEGTQDVAVNDVIAVLAGDGEDVKAAGAGAAGAPPKAASPAQT
                     PAAAKPAPTPAPAAAPAAKAAAPAPQAAVPASQANGHARIFSSPLARRLAKEAGIELS
                     RINGSGPHGRVIARDVEEARSGKGLKAPAAAPAGAPRIAPSMSDKQIRALYEDGSYEV
                     IPHDGMRRTIAQRLTASVQTIPHFYLTMDCNIGKLVEAREEINAAAPKDKDGKPAYKL
                     SVNDFVIKALAIALQRIPNANVSWTDGGMLKHRHSDVGVAVAMPGGLITPIIRKAETK
                     PISVISAEMKDFAARARARKLKPEEYQGGTTAVSNLGMYGIKDFTAVINPPHATILAV
                     GTGEERAIVKHGKIEVAQIMSVTMSCDHRAVDGALGAELIGAFKLLIENPVMMVV"
     gene            1302..2720
                     /gene="lpdA"
                     /locus_tag="VN968_09900"
     CDS             1302..2720
                     /gene="lpdA"
                     /locus_tag="VN968_09900"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXL29585.1"
                     /translation="MPDTSYDIIIIGSGPGGYVTAIRAAQLGFKTAIVEKSYLGGICL
                     NWGCIPTKALLRSAEIFHYMQYAKDYGLSADNVSFDPKAVVQRSRGVSKRLNDGVGFL
                     MKKNKVTVIWGDASIDAPGKITVKKSTAEAPKGALGEGAYQAKHIILATGARPRVLPG
                     LEPDKKLIWTYFEAMIPEKMPKSLLVVGSGAIGIEFASFFHTMGAEVTVVEVLPQILP
                     VEDAEIAGLARKRFEKQGIKILTNTKVTKLEKKTDSVVATIDDGKKPQTVEFDRVISA
                     VGVVGNIENLGLEKLGVKTERGCVVIDGYGKTSVPGIYAIGDVSGPPMLAHKAEHEGV
                     VCVEAIKGLHPHAMDKNLIPGCTYCNPQVASVGLTEASAKEGGREIRVGRFPFVGNGK
                     AIALGEDQGLVKVVFDKKTGQLLGAHMIGAEVTELIQGYVVAMNLETTEEELMHTVFP
                     HPTLSEMMKEAVLDAYGRVLNI"
     gene            2757..3998
                     /locus_tag="VN968_09905"
     CDS             2757..3998
                     /locus_tag="VN968_09905"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXL29586.1"
                     /translation="MKDNDNLTIERPTFVTHLECAMEGDHYPADQVHNLSRAGKPLLV
                     RYDLAAVKQALTKDALAQRPADLWRYREMLPVRKIADIVSLGEVTTPLIRLPKLAKKL
                     GGGEIIVKDEGRLPTGSFKARGLVMAVSMGKALGIRHMAMPTNGNAGAALAAYATGCG
                     IKTTIFCPADTPEVNVSEIELQGATVYRVNGLIDDCGKIVGEGKAKAGWFDTSTLKEP
                     YRIEGKKTMGLELAEQLGWDVPDVIFYPTGGGTGLIGMWKAFAELEAIGFIGSKRPRM
                     VAVQAAGCAPMVRAFENGTEHAARWEDAHTIASGIRVPQAIGDFLILRAVRESKGFAI
                     AVTDETISAALNEVAREEGLLPCPEGAATYAAYKQSLADGRVTKNDRVMLFNCATGLK
                     YPLPPVTRTLDRHQPIDYAKL"
     gene            4084..5055
                     /locus_tag="VN968_09910"
     CDS             4084..5055
                     /locus_tag="VN968_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugD"
                     /protein_id="HXL29587.1"
                     /translation="MRRTGWAALIALLISGGSALSENFPSHPITIIVPFSAGGPSDAM
                     ARILAERMKARLGEALLIENVTGAGGSIGVGRAVRSPPDGYTISFGHLGTHVANGAIY
                     KLGYDLVTDLEPVVLLPSNPMIVVSKNAVPAKSLKELIEWLKARPTPATAGTAGAGSG
                     SHIAGLYFENVTGIKLQYVPYRGTAPAMNDLIAGQIDVIVDQTSNSISQVRAGNIRAY
                     AITADKRLEQASDVPTTDEAGLPGFHMTLWSGLWVPKDTPKDIVAKLNASAVDALNDP
                     AVRRQLENLGLQMPPADQLSPEALGSWQKAEIAKWWPMLKAANVKVD"
BASE COUNT         1051 a         1622 c         1681 g          837 t
ORIGIN      
        1 tggtgcccga aggcacgcag gatgtcgccg tcaacgacgt gatcgcggtg ttggccggcg
       61 atggcgagga cgtcaaggcg gcgggggcag gggctgccgg cgcgccgccg aaggcagcgt
      121 cacctgcgca aacacccgct gctgcgaagc cggcgccgac accggcaccc gctgcggctc
      181 ctgcggcaaa ggccgcagct cccgcgcctc aggcggcggt accggcgtcg caggccaacg
      241 gccatgcccg gattttctcg tcgccgctgg cgcgacgcct ggcaaaggaa gccggcattg
      301 agctctcgcg catcaacggt tccggcccgc acggccgcgt catcgcgcgc gacgtcgagg
      361 aagccagatc cggcaagggg ctgaaagccc cggctgccgc gcccgccggc gcgccgcgca
      421 ttgctccatc gatgtcggac aagcagatcc gcgcgcttta cgaggacggc agttacgagg
      481 tgatcccgca tgacggcatg cgccgcacca ttgcgcagcg cctcaccgcc tcggtgcaga
      541 ccattccgca tttctacctg accatggact gcaacatcgg caagctggtc gaggcccgcg
      601 aggagatcaa cgccgcggcg cccaaggaca aggacggcaa gccggcctac aagctctcgg
      661 tcaacgattt cgtcatcaag gcgctggcga tcgcgctgca gcggattccc aacgccaatg
      721 tcagctggac cgacggcggc atgctcaaac acaggcattc cgatgtcggc gtggccgtgg
      781 cgatgccggg cggcctgatc acgccgatca tccgcaaagc cgagaccaag ccgatctcgg
      841 tcatctccgc cgagatgaag gatttcgccg cccgcgcccg cgcccgcaaa ctcaagccgg
      901 aggaatatca gggcggcacc acggccgtct ccaacctcgg catgtacggc atcaaggact
      961 ttaccgcggt gatcaatccg ccgcacgcca ccattttggc ggtcggcacc ggcgaggagc
     1021 gcgccatcgt caagcacggc aagatcgaag tcgcgcagat catgagcgtg acgatgtcct
     1081 gcgaccaccg cgcagtcgac ggcgcgctcg gcgccgagct gatcggggcc ttcaagttgc
     1141 tgatcgagaa tccggtcatg atggtggtgt gagagtgagg tgcttcacgc cgtcattgcg
     1201 agcgcagcga agcaatccat ggcgaggcgg gcaagctgga ttgcttcgtc gcaactgctc
     1261 ctcgcaatga cgaagcacac gatgagaacg agcgagtaaa catgcccgat acatcctacg
     1321 acatcatcat catcggttcc ggtcccggcg gctatgtcac cgcgatccgc gcggcgcagc
     1381 tcggtttcaa gaccgcgatc gtggaaaaat cctatctcgg cggcatctgc ctgaactggg
     1441 gctgcatccc gaccaaggcg ctgctgcgct cggccgagat ctttcattac atgcagtacg
     1501 ccaaggatta cggcctttcg gccgacaacg tgtcgttcga tccaaaggcc gtggtgcagc
     1561 gctcgcgcgg cgtctcaaag cgcctcaacg acggcgtcgg ctttctgatg aagaagaaca
     1621 aggtcacggt catctggggt gatgcctcga tcgacgcgcc cggcaagatc acggtcaaga
     1681 aatcgaccgc cgaagccccc aagggcgcgc tcggcgaggg cgcctatcaa gcaaaacaca
     1741 ttattctggc gaccggcgcg cggccgcgcg tgctgccggg gctcgagccc gacaagaaac
     1801 tgatctggac ttacttcgag gcgatgatcc ccgagaagat gccgaagtcg ctactggtgg
     1861 tcggctccgg cgcgatcggg attgaattcg cgtcgttctt ccacaccatg ggcgccgagg
     1921 tgaccgtggt cgaggtgctg ccgcagatat tgccggtgga agatgccgaa atcgcaggtt
     1981 tggcgcgcaa gcggttcgaa aagcagggca tcaagatcct caccaatacc aaggtgacaa
     2041 aactcgagaa aaagaccgac agcgtggtcg ccaccatcga cgacggcaag aagccgcaga
     2101 cggtcgaatt cgaccgggtg atttcggctg tcggcgtggt cggcaatatc gaaaatctcg
     2161 ggttggaaaa actcggcgtc aagaccgagc gcggctgcgt cgtgatcgac ggttacggca
     2221 agaccagcgt gcccggcatc tacgccatcg gcgacgtctc aggtcccccg atgctggcgc
     2281 acaaggccga gcatgaaggc gtggtgtgcg tcgaggccat caagggcctg cacccgcatg
     2341 ccatggacaa gaacctcatt ccgggctgca cctattgcaa tccgcaggta gcctcggtcg
     2401 gcctcaccga ggcgtcggca aaggagggcg gccgtgagat ccgtgtcggg cgttttccgt
     2461 ttgtcggcaa cggcaaggcg atcgcactgg gtgaggacca gggcctggtc aaggtggtct
     2521 tcgacaagaa gaccggacag ttgctcgggg cgcatatgat cggcgcagaa gtgaccgaac
     2581 tgatccaggg ctacgtggtt gccatgaacc tcgaaaccac ggaagaagaa ctgatgcaca
     2641 ccgtgttccc gcacccgacg ctgtcagaga tgatgaagga agcggtgctg gacgcctacg
     2701 gccgcgtcct gaacatctag acgcgtgctg aacatctaca aaagagaccg tatcccgtga
     2761 aagacaatga caatctgacg atcgaacgtc cgacgttcgt gacccatctc gaatgcgcga
     2821 tggaaggcga tcactacccg gccgaccagg tccataacct ctccagggcc ggcaagccgc
     2881 tgctggtgcg ctacgacctt gccgccgtga agcaggcttt gaccaaagac gcgctggcac
     2941 agcggcccgc cgatctatgg cgttaccgcg agatgctgcc ggtgcgcaaa atcgccgaca
     3001 tcgtcagcct cggcgaggtg acgacgccac tgatccgcct tccgaagctc gccaaaaaac
     3061 tcggcggcgg cgaaatcatc gtgaaagatg aaggccggtt gccgaccggc tcgttcaagg
     3121 cacgcgggct tgtcatggcg gtgtcgatgg gcaaggcgct cggcatcagg cacatggcga
     3181 tgccgaccaa cggcaatgcc ggtgcggcgc tcgcggccta cgcgaccggc tgcggcatca
     3241 agaccacgat cttctgcccg gcggatacgc ccgaagtgaa tgtcagcgag atcgagctgc
     3301 agggcgccac cgtctaccgc gtcaacggac tgatcgacga ttgcggcaag atcgtcggcg
     3361 agggcaaggc caaagccggc tggttcgaca cctccacgct gaaggagccg taccggatcg
     3421 agggcaagaa gacgatgggg ctggaacttg ccgagcagct cggctgggac gtcccggacg
     3481 tgatcttcta tccgaccggc ggcggcaccg gcctgatcgg catgtggaag gcgttcgccg
     3541 agctcgaagc catcgggttc atcggctcga agcggccgcg gatggtggcg gtgcaggcgg
     3601 cgggctgtgc gccgatggtg cgcgcctttg agaacggcac cgagcacgcc gcccgctggg
     3661 aggatgccca caccatcgcg tccggcatcc gcgttcccca ggcgatcgga gattttctta
     3721 ttctgcgcgc ggtgcgcgag agcaagggct ttgcgattgc ggtgacggat gaaacgattt
     3781 ccgctgcgct gaacgaggtc gcgcgtgaag aaggcttgtt gccgtgcccg gaaggcgcgg
     3841 caacctatgc cgcctacaaa caaagtctcg ccgacgggcg ggtgacgaaa aatgaccgcg
     3901 tgatgctgtt caactgcgcg acaggcctca aatacccgtt gccgccggtc acgcgcacgc
     3961 tcgaccgcca ccagccgatc gactacgcga agctgtagct atcgccaagg cgaagctgta
     4021 gctatcgcaa cgcgaagcgg tagctatcgc agcttgaatg gcgcggcagg gttggagggt
     4081 gtgatgcgaa gaacaggttg ggccgcgttg attgcgctct tgatatcggg cggcagcgcg
     4141 ctctcggaaa actttccatc gcatcccatc accatcatcg tgccgttttc cgccggcgga
     4201 ccgtccgatg cgatggcgcg gattctggcc gagcgcatga aggccaggct cggcgaagcc
     4261 ctactgatcg agaacgtgac gggcgcgggc ggctcgatcg gcgtcggacg cgcggtgcgc
     4321 tcgccgcccg atggctacac catcagcttc ggccatctcg gcacccacgt cgccaacggc
     4381 gcgatctaca agctcggcta cgacctcgtc accgatctcg agccggtggt gctgctgccg
     4441 agcaatccga tgatcgtcgt cagcaagaac gcggttccgg ccaaatcgct gaaagagctg
     4501 attgagtggt tgaaagcgcg cccgacgccg gcgacggccg gcaccgccgg cgccggctcg
     4561 ggaagtcaca tcgccgggct gtattttgag aacgtcaccg gcatcaagct gcaatatgtg
     4621 ccgtatcgcg gcacggcgcc ggcgatgaat gatctcatcg ccggacagat cgatgtgatc
     4681 gtcgatcaga cctccaattc catcagccag gtgcgcgccg gtaacatccg cgcctatgcc
     4741 atcaccgccg acaagcgcct cgagcaggca tcggacgttc caaccaccga cgaagcgggt
     4801 ctgcccgggt ttcacatgac gttgtggtcc ggcctgtggg tgccgaaaga cacgccaaaa
     4861 gacatcgtcg caaagctcaa cgcttcggcc gtggacgcgc tgaacgaccc ggcggtgcgc
     4921 aggcaactcg aaaacctggg gctgcagatg ccaccggccg atcagctctc gccggaagcg
     4981 ctcggcagct ggcaaaaggc cgagatcgcg aaatggtggc cgatgctcaa ggctgccaac
     5041 gtcaaggtgg actagtccgg ggcgcgaact agtggttaca atctgatact tggtgccggc
     5101 gcagagacgc caccggaggc ccatgtgccg tcgccgcgcc atgccagctg gcagacgttc
     5161 cgaatgtctc tggaggtgga ttgggaggtg g
//
LOCUS       DATNOI010000257        22845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1458489,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000257 DATNOI010000000
VERSION     DATNOI010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 22845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22845
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458489"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1188)
                     /locus_tag="VN968_09915"
     CDS             complement(1..1188)
                     /locus_tag="VN968_09915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXL29588.1"
                     /translation="MSADLMDDFPPSRDGLVTLANWRKAPFSAWGFRNVRRLIPTANI
                     AASNHAAGLEMSLEDIGHIGFTSHDGNPTTISRALRTTNTDAFVVLRRGRLAMEWYDN
                     GMSATTPHIVFSVSKSICGTLGGILWGRGLLDPDDRVLDYIPELASSVYAGCTIRHLL
                     DMAVGIAFNEDYEDPRGDVVRYRHAAGWDPLPAGEPPIDLRSFLGQQTPDGRKHGGLF
                     HYVSTNTDVLGWVYERACGQPYADILSEYLWVPLGAERDAYITVDARDAMRAAGGICV
                     APRDLARFGEMIRRRGIADGRQVVPGAWIDDINCNGDPEAWAASEFAEIFPKGSYRSQ
                     WYRIDRDRGVLVAFGIHGQFIYIHPQAELVIVRMASEATPLDPDRARGWRRGFDAIAE
                     EFM"
     gene            1478..2188
                     /locus_tag="VN968_09920"
     CDS             1478..2188
                     /locus_tag="VN968_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXL29589.1"
                     /translation="MRRILLGAAAMIVVAIPALAADLPARKYTKAPAYTAPEAVYNWT
                     GFYIGGNLGGAFAGNNSLQGSGGRFMGGVEGGFDYQFAPNWVVGAEAQYDWLSNNNNG
                     RLFPGGVLVTSKNNELGSVTGRVGYTWGPALLYGKGGFAFRDNNNLGVTVAGVPAGFT
                     TNGKRQDGYTVGAGLEYMFAPSWSAKAEYQYYNFGSTTFTTGPAAIVGTRFKDDEHTV
                     KAGINYRFGWGGPVAAHY"
     gene            2440..2679
                     /locus_tag="VN968_09925"
     CDS             2440..2679
                     /locus_tag="VN968_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29590.1"
                     /translation="MRVFALALILCGVAVPCLAQTVLKSEPLVLAPYEIAFVQDASCP
                     VGKVLKVTGAIRGLHRRKACVALAAEQASLAKATP"
     gene            complement(2761..3015)
                     /locus_tag="VN968_09930"
     CDS             complement(2761..3015)
                     /locus_tag="VN968_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29591.1"
                     /translation="MPYALFCNEARLSKTYPTEADVWKLARQSGLVVDVVMGEENRYP
                     RPVLDNDYEIRPCRPDPHEDPARNQAEAERDAELEFQLNS"
     gene            3551..3919
                     /locus_tag="VN968_09935"
     CDS             3551..3919
                     /locus_tag="VN968_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29592.1"
                     /translation="MIETLKARVNGDEALVRRGRFLTTTFLLEVGQTSWLISIFEGKV
                     LSVTPGPFVMPSSSFSLRAPEQEWEKFWSSRPPPGSNDLMALIKRRVLKAEGNLQVFM
                     ANLRYFKEALAKLRPEGVKA"
     gene            3916..4764
                     /locus_tag="VN968_09940"
     CDS             3916..4764
                     /locus_tag="VN968_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL29593.1"
                     /translation="MSARFEPIVGRYMHLELFGRPHRIYVEEAGEGTPLLCLHTAGAD
                     GRQYRALMNDARITRGHRVIAFDMPWHGKSSPPSGWHNEEYQLTSAHYTTMILKIMTA
                     LELDRPILIGCSIGGRIALHLALEHPQQFRAIIGLQAGAHVDPYYDLNFLHRPDVHGG
                     EVCAAIVSGLVGPDAPDAERWETLWHYMQGGPGVFKGDLYFYTLDGDIRGRVAEIDTS
                     RCPLFLLSGEYDYSCTPEETLGVASSIQGCEVTIMKGLGHFPMSENPTEFLKHLLPVL
                     EKIKGR"
     gene            complement(5453..7036)
                     /locus_tag="VN968_09945"
     CDS             complement(5453..7036)
                     /locus_tag="VN968_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXL29594.1"
                     /translation="MTRIVSTAAVRAALIEGREIALLDVREEGPYSRGHPLFAVSLPL
                     SRLELKVLDLVPRFDAPIVLYDDGGGLVERAEARLRALGYTDVARLDGGLDGWRHAGG
                     EVFIDVNVPSKAFGELVESKRHTPSLSAREVDRLIAEKADVVVLDSRRFEEYRNMSIP
                     TGTSVPGGELVLRLQDLAPRPETTVIVNCAGRTRSIIGTQSLINAGIPNPVFALRNGT
                     IGWTLAGLDLDRGAGRRFPKLSAEARATALNRARALAERAGIRSVGRDTVEGWRREPR
                     GFTLFLLDVRNPEEFEAGHLPGFRSAPGGQLVQATDEWVGVRHAHLVLADDDGVRAML
                     AASWLIQMGWRNVHVLDNGIAGDELEIGPRPPRRPPFPAADVLPLAPEALAAELDETV
                     VVDLATSPRHIAGHVPGAWFVVRARLAEALHRLPAGRRPVLTSCDGTLARYAAADLAV
                     LPDRPVAILAGGTRAWEAAGLPIERGLTRAADAVDDVYKRPYEGTDNAAAAMQAYLDW
                     EFGLVEQLARDGTHGFEVF"
     gene            7494..8531
                     /locus_tag="VN968_09950"
     CDS             7494..8531
                     /locus_tag="VN968_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative FMN-dependent luciferase-like
                     monooxygenase"
                     /protein_id="HXL29595.1"
                     /translation="MKGSVSSKRLGFFTRLLDDAAPADRYRLAAAQIRHAESAGFDSA
                     WIAQHHFHEGEGGLPAPFAFLGFVAAQTCRIRLGTGIVTLPLENPVRVAEDAAVLDLL
                     SNGRLELGVGTGGNPSAFAAFGLDSAERAEIFARNLDIVRNALAGGPLAGGDTLYPPR
                     PQLCDRIWQATFSVSGGTRAGKAGDGLLLSRTQPRSGDAPRASLAEIQHPIIDAYLDA
                     LPPGREPRIMASRSVFAADDRQEAFRLADIGLRRARDHFVASGHASPGDALTDMIAAF
                     DVHVGTPEDVIVSLHADSTLSRVTDLVFQAHSIDPPHRYILRSIELIAETVAPALGWA
                     KRTRADAALVE"
     gene            8559..9194
                     /locus_tag="VN968_09955"
     CDS             8559..9194
                     /locus_tag="VN968_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain protein"
                     /protein_id="HXL29596.1"
                     /translation="MSTEPKDVIDLLAGIEPGSSLDGIRARRLQARENAQKSYLALFE
                     PADIGDVAVAERYAVAAFVAGIHAEPAAASFYSAGFARSADRSELVDVLGAEIERART
                     EGPYGAFPAGLLSVEVKGGLIHRIAGKSRSVLGGRLAAALEHAHLLVFHPRDANPAAI
                     RVLLEAGWSNTAIVTLSQLVAFLCFQIRTIAGLRTLGASLGHGASVSAKAS"
     gene            9210..9818
                     /locus_tag="VN968_09960"
     CDS             9210..9818
                     /locus_tag="VN968_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylhydroperoxidase domain protein"
                     /protein_id="HXL29597.1"
                     /translation="MSESIVVRASKDQPVTFTQEELGWLPWLDPLPEAEFTERHWAGL
                     VERSRAKSDYFRLLVRDPQILEARTKTDKDIFYNIADGLPRAERELAAAATSRYNGCI
                     YCTSVHARFATVHSKRRDDIQRLLDQGVGADLGERWNAIVSASVALASTPSAFGQEHI
                     EQLRGVGLNDAEIVDVINGAAFFNWANRLMLSLGEPSTVVDA"
     gene            9880..10629
                     /locus_tag="VN968_09965"
     CDS             9880..10629
                     /locus_tag="VN968_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL29598.1"
                     /translation="MAFALDHVVIAVNDLDRAVADYQSLGFTVYPGGVHHGGVSHNAL
                     IVFEDGSYFEIIAYREAAPANRWWHVLTTAGEGIVDFAVLPENTEKDLGAARARGLNL
                     EGPTPGGRLRLDGVRLDWQIVRPATTDLPFWCGDVTPRHLRVPEGAIRHHANGVTGIS
                     RVRILVSDAVTSTERYRALVGPDAVSLENGIARINLESTVFELVVPDNDPARGHLNSR
                     GEGVWSLELRGVAAKALDPKRLHGADLAIVG"
     gene            complement(10647..12281)
                     /locus_tag="VN968_09970"
     CDS             complement(10647..12281)
                     /locus_tag="VN968_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL29599.1"
                     /translation="MTGLLAIANLTVSLPPGADRPFALDDVSIDLRRGEIHCLIGESG
                     SGKSMIANAILGLLPENVRVTAGDIRFEEISLLTLGEQEMRKIRGAHIGMIFQEPMTA
                     LNPLRSIGDQIGEMFWTHTDLDSATIRTRVLSLLSDVQIAQPEEIERAYPHELSGGQR
                     QRAMIAMALALEPAILVADEPTTALDVTTQAQILALIRDLQARKGTSVLFITHDFGVV
                     AEIADRVTVLQHGRVVEHGARDQLLNHPSEPYTQELIAAVPSYLPPPSSAADGEKILT
                     ISHVSKTYRQFGLLRRSRRVTQALKDVSASLTKGTTLGIVGESGSGKSTLARCIVRLI
                     DPDRGVIRLGDAELTSMSRRDLRAQSRHIQMVFQDPYGSLNPRRTAGELVTQGLILHG
                     MPGPQARAEAKQMFGVVGLDPQAMDRYPHEFSGGQRQRIGLARALALRPDVLVADEPV
                     SALDVSVQAQILRLLANLRRTFGLSMIFITHDLRVAAQICDRVAVMKDGEIVEHGATA
                     EVFASPQHDYTRLLLRSIPGQRWVPQAAGSYLATAG"
     gene            complement(12278..13108)
                     /locus_tag="VN968_09975"
     CDS             complement(12278..13108)
                     /locus_tag="VN968_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL29600.1"
                     /translation="MQTLTRFLGNPVALIGLGLLVVVTAMALSAASLFPNNPLDLAGR
                     PLQWPLSNPRFWLGTDVQGRDIAAVMFYGARVSLLIGVVATLIAMTIGILIGALAGYY
                     GGIADEMLMRLTEAFQTLPNFLLLLVLVAVFGSDIKTVTIAIGLVSWPASARLTRAEF
                     LSLRNREFVEACRTIGMRDMQIIFREILPNAMPPVIVYASVVMAVAILLESALAFLNL
                     SDPNVPSWGNLIGGGRAVLRTQWYVSAIPGVAILMTVLAVSLIGQGLNDALNPRLKRR
                     "
     gene            complement(13114..14085)
                     /locus_tag="VN968_09980"
     CDS             complement(13114..14085)
                     /locus_tag="VN968_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL29601.1"
                     /translation="MRFFLFAIRRLAASVPTLLIVLAGLFMLLQFAPGDTVDALVAQM
                     GGGDSAMMEQLRKYYGLDATATVQLIRYLTRLVQLDLGFSAIYGKPVIDVILERLSAT
                     LLLMASSLSFAFAGGLVLGVLAARRVNQWPDGVISTIGAIFYATPAFWLGLMGIIIFA
                     VNLRVLPAGGLEEIGAGYTGLRRAVDIVWHLALPTATLGLIFLATYLRIMRASMLEVM
                     TLDFVRTARAKGLAKAAVLRRHVLRNALLPMVTLVGLQAGTMLGGSVVVESVFALPGL
                     GRLAYEAVVQRDLNTLLGIVFVSALLVICVNFLVDILYARLDPRIAN"
     gene            complement(14286..15881)
                     /locus_tag="VN968_09985"
     CDS             complement(14286..15881)
                     /locus_tag="VN968_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL29602.1"
                     /translation="MRLTRRDLLVSTAAAAYAATRPDVSWAQEQPKRGGTLSVHLPGE
                     QRILNPAIRASTGVYIVTSKIVESLVDLGADGKPTPVLATSWEAAPDGKTVTFKLREG
                     VNWHDGKPFTSADVQYTALELWKKYLNYGTQLQQYLEAVDTPDDHTAVCRYSRPMPLP
                     LLLRALADLGYIVPRHVFEGTNILENPANTAPIGTGPFKFVQYERGQFIIAERNPNYW
                     RKEFPYLDRIVWRVIADKSAASAALETGQIQLSTYSGLSLADYDRLKKDARFDISSRG
                     IEANAFNNTLEFNVRRKELSDVRVRRAIVHAIDVPFFIENFLYGRGKPATGPIPSSSP
                     AFYPGGEVPYAHDLKKAEALLDEAGYKRGADGIRFALKIVPIQNGEDVPLLATYIQQS
                     LSKIGIKIDIVNLDIAGALSAVYRDWNFDLATGWHQYRGDPAVSTTVWYRSGSPKGAP
                     WTNQWGWQSDAVDKLIDEAASELDTGKRKLLYADWVKVINEELPIWMVTERQFIAATS
                     KQVQNHHTSPRWDSGDWHDTWIS"
     gene            complement(16168..16593)
                     /locus_tag="VN968_09990"
     CDS             complement(16168..16593)
                     /locus_tag="VN968_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HXL29603.1"
                     /translation="MSDVTAVAAPRPIAEIASYHAHVYYDPAATRSTAEQLRAWIGER
                     FPVQLGRWHDARIGPHDAAMFQVAFANDVFATLVPWLMLNHGGLSILIHPNTANPRRD
                     HVIDPFWIGTRLAVHDEKLPVESEPEGAPQPNTTPHISP"
     gene            complement(16796..17584)
                     /locus_tag="VN968_09995"
     CDS             complement(16796..17584)
                     /locus_tag="VN968_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXL29604.1"
                     /translation="MKKLLLASTGFLALVASAPAFAADLPARPYTKAPPAMVATINDW
                     SGFYVGFNAGGGSSRDRWNLLTNGGVAVNPALSNEGTTNASGAAVGGQIGYRYQVSNW
                     VFGVEAQGDWSSFKGNSRNLILPTLTDRTRVNSFGLFTGQVGYSWSPSFLAYLKGGAA
                     VVGDRYDTFTTATGVMVDRASTTRWGGTVGAGLEYAFAPNWSVGVEYDHLFMGNKNLN
                     FVTAAGLTTMTHRINQDIDIGLVRVNYKFGPSGMFGPGGTFGKY"
     gene            18200..19225
                     /locus_tag="VN968_10000"
     CDS             18200..19225
                     /locus_tag="VN968_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HXL29605.1"
                     /translation="MKAVYTELHRSHDPQFFLVRGVVKRTTEQPERADRLLAGLRAGK
                     HDLVTPSAFGQGPRARVHSPEYLGFLAEAWDAWTELGDCGPEMIANMHPVRNAATYPT
                     HIVGRLGWHTIDTSCPIGPGTWAAACAATDVATTAAQLVFDGEDAAYALCRPPGHHAY
                     RDLASGFCFLNNSAIAAAHLRGRHERVAILDVDVHHGNGTQGIFYERPDVLTVSIHAD
                     PAFFNPFVWGYAHERGAGPGLGANLNLPLPIGTGDDGYTQALGAAEKTIRAFAPGALV
                     VALGLDASEHDPLAGLAVTTDGFRRIGGAIARLGLPTVFVQEGGYLSEILGTNLTAAL
                     AGFEEVR"
     gene            complement(19268..19867)
                     /locus_tag="VN968_10005"
     CDS             complement(19268..19867)
                     /locus_tag="VN968_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29606.1"
                     /translation="MRSRKPKPQEPTATATVAFHEAGHALASVLAFRAIGSGRRAIKY
                     VEISEGEMPAGQWSGSCRGPDIYSPAWPIARIALQNRRKMEWQIVIDLAGPIAEAVAR
                     GERRKKHVGAFALFNCGSSEDFSHAGKVIADLCALTKRRQAFPRFEQRALAVVLAHWP
                     AVSMLAAALIETGRLDGDQVEQIVTAGVRRRDDQAAIPL"
     gene            20738..21022
                     /locus_tag="VN968_10010"
     CDS             20738..21022
                     /locus_tag="VN968_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29607.1"
                     /translation="MKSALDCEAFVGVIIQRIAPKSRTDPNWAVRGVRFGKADRDKSG
                     EVLATVVEQMQREFSLTDNQDDAPQTKDAKPEVIQICRDEERASAASGYD"
     gene            complement(21358..21939)
                     /locus_tag="VN968_10015"
     CDS             complement(21358..21939)
                     /locus_tag="VN968_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29608.1"
                     /translation="MHRLSSSFVLGFHGCDQSVADDLLNGKDFVPSANDWDWLGSGIY
                     FWETNPVRGIEFAKTGRSRGKITAPAVVGAVIDLGFCLDLISSTGVAAVKAAHEDFIA
                     YAKKADKPVPKNRLGPDRLLRDLDCAVINHLHKVRDAAKLPAFQTVKAVFIEGGRIYE
                     EAGFFEKTHIQICVREKSCIKGVFRVSDDYLTA"
     gene            complement(21940..22104)
                     /locus_tag="VN968_10020"
     CDS             complement(21940..22104)
                     /locus_tag="VN968_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29609.1"
                     /translation="MSEQELREFKAAMAKLRLEHTSSREKATQFLKEEGFLDENGEVS
                     KRYSAAVAAA"
     gene            complement(22227..>22845)
                     /locus_tag="VN968_10025"
     CDS             complement(22227..>22845)
                     /locus_tag="VN968_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXL29610.1"
                     /translation="IWYTVGIFGGTPGVLRFDPVTGLSEVYNVPAPAFGVRGGDIDKN
                     GVVWASLSSGHLGSFDRRKCKGPLNGPKATGNHCPEGWTIYQYPGPGFEGVGENSAES
                     SYYTWVDQHNTSGLGENIPMSTANLGDGFVALKDGNMITIRIPYPLSFYAKGFDGRID
                     DPNAGWKGRGLWSTNGDRTPWLKEGGKGARPRAVHIQFRPDPLAN"
BASE COUNT         4373 a         6965 c         7106 g         4401 t
ORIGIN      
        1 ctacatgaat tcttccgcga tcgcatcaaa gccgcggcgc cagccccgcg cgcgatccgg
       61 atcgagtggc gttgcttccg aagccatcct cacgatcacg agttccgcct gcggatggat
      121 gtagatgaat tgcccgtgga tgccgaatgc caccagcacc ccgcggtcgc ggtcgatgcg
      181 ataccattgg ctgcgatagc tgcctttcgg aaatatctcg gcgaattcgg aagccgccca
      241 tgcctcggga tcgccgttac agttgatatc gtcgatccac gcgccgggta ccacctgacg
      301 gccatccgcg atgccgcgcc ggcgaatcat ctcgccgaag cgggcgaggt cacgcggcgc
      361 gacgcagatg ccgccggcgg cgcgcatcgc gtcgcgggcg tcgacggtga tataggcgtc
      421 gcgctccgcg ccgagcggca cccacaaata ttcgctcagg atgtcggcgt agggctgacc
      481 gcaggcgcgc tcatagaccc agccgagcac gtcggtattg gtcgatacat agtgaaacag
      541 tccgccgtgt ttccgtccgt ccggcgtttg ctggccgaga aaactgcgca gatcgatcgg
      601 cggttcgccc gcgggcagcg gatcccagcc cgcggcgtgg cggtagcgca ccacgtcgcc
      661 cctggggtct tcataatcct cgttgaaggc gatgccgacc gccatatcca ggagatgccg
      721 gattgtgcag ccggcgtaga ccgacgaggc cagttcaggg atgtaatcga ggacgcggtc
      781 atccggatcg agcaagccgc ggccccagag aattccgccg agcgtgccgc aaatcgactt
      841 gctcacggaa aagacaatgt gcggcgtagt ggcgctcatg ccgttgtcgt accactccat
      901 cgcgagccgc ccccgcctga gcacgacgaa cgcatcggta ttggtcgtgc gcagcgcgcg
      961 gctgatcgtg gtcgggttgc cgtcatggct ggtgaaaccg atatgtccga tatcttccag
     1021 agacatttcg agtcccgccg catgattgga ggccgcgatg ttggcggtcg gaatcaagcg
     1081 gcggacgttg cgaaagcccc aggcgctgaa cggcgccttg cgccagttcg ccagcgtcac
     1141 cagtccgtcg cgcgaaggcg gaaaatcgtc catcaaatcg gcggacattt tgtctcacct
     1201 cattcttgtg cttttggcgg atttgcgggt accggcgcgt gaactgcgat cacttttcgg
     1261 cgaggctcga acctgactat tgccatcgct gacacagaac atagccataa accactccgg
     1321 tagcgggcga aaagccggcc agcgcggtgg aacaaaatgg cactgttgcc gccccgccac
     1381 agccggcagc catcggtact ccggcttcgc cgcgaagcaa ccaaactgag gccatgttgt
     1441 ttgatgaggg tcgcaacaca gtaacgggga accagtgatg agaagaattt tgctcggcgc
     1501 tgccgctatg atcgtggtcg cgattccagc actggctgcc gatttgccgg cacgtaaata
     1561 taccaaggcc ccagcctata ccgcgccgga agcagtctat aactggaccg ggttctacat
     1621 cggcggcaat ctgggcggcg cgttcgctgg caacaacagc ctacaaggaa gcggcggccg
     1681 gttcatgggc ggcgtcgagg gcggcttcga ctatcagttc gccccgaact gggtggttgg
     1741 tgcggaagcc cagtatgact ggctgtccaa caacaacaat ggccgtcttt tcccgggtgg
     1801 cgttctggta acctcgaaaa ataacgaact tggctcggtc acgggccggg tcggctacac
     1861 ttggggtccg gcgctgctgt acggcaaggg cggcttcgcg ttccgcgaca ataacaattt
     1921 gggcgttacc gtcgccggcg ttccggccgg atttaccacc aatggaaaac gccaggacgg
     1981 ctacaccgtt ggcgccggtc tcgaatacat gtttgcgccc agctggtccg ccaaggccga
     2041 ataccagtat tacaacttcg gcagcaccac ctttacgacg ggacccgccg cgatcgtcgg
     2101 cacccggttc aaggacgacg agcacaccgt caaggccggc atcaactacc gctttggctg
     2161 gggtggccct gtcgcggcgc actactgaga caggccgctt cgatgcgacc aagggccggc
     2221 tccgcgccgg ccttttttat gggcgaaggc cgtccgggta aggcgggcag gaccgtcacg
     2281 tggcgcatcg tgcgccagag aagtcgcgca agccattgcg gcagagcgtc ttccatcgcc
     2341 ggaccggatt gcactttcga cccaaaaata aattttggcg tctgtgaaac ttctgctcgc
     2401 gataggctat tgtactgcgg gcagtggtgg acaacgaaca tgcgggtttt tgcgttagcg
     2461 ctgattttat gcggggttgc ggtgccgtgc ctggctcaga ccgtcctgaa atccgaacca
     2521 ctggtgcttg cgccctatga aatcgccttc gtgcaggatg cttcatgtcc ggtcggcaag
     2581 gtgctcaagg tcaccggcgc gatcagaggc ttgcaccgca ggaaggcctg cgtggcgctc
     2641 gccgccgaac aggcgtcgct ggccaaggca acgccctagg atttggcctc gcggcgtcgc
     2701 tccagctctc tgcaaaaatt gaaacgtcag ggattgtggg ccccggtgtt cgccgggtcc
     2761 tcacgaattc agctggaatt ccagttcggc gtcgcgctcg gcttcggcct ggttcctggc
     2821 gggatcctcg tgcgggtccg gccggcaggg cctgatttcg tagtcgttgt ccagcacagg
     2881 tcgcgggtac cggttttctt cgcccatcac cacgtccacc accagcccac tctgccgggc
     2941 gagtttccag acatccgcct cggtcggata ggtcttgctc agcctggcct cgttgcaaaa
     3001 cagcgcgtaa ggcatcgtat cgctccgctc atcatccatc ttaacgccca aggcgatgtt
     3061 gggttgcaac ggagcgcgcc aatgtgacgg gaagacggcc tgcgaaagct atttcggtgc
     3121 atcgtcgaac cggagggcag tcaggctcgc ttcgccaaat tcaagcgatg tcaaggcggg
     3181 cttcgaagaa tcggtaatgc ggccgacccg cgccaactgg gccggccgtt gccggggaat
     3241 cccgggcggg ggctttttga caggagtccc tgagtcgtaa acgagacctg aatcacccgg
     3301 aaaataatcc ccgggactct ccggctagaa tcgcccgatg ttttgcaggc catgacaatc
     3361 attctgcaaa actcctgcgg gcgcgtgcat ctcacgctca ttgttgcgct gctggcggca
     3421 tgcgcggcaa acctggtgct ggcatgcgcc tggctttagc cgattgacgc cgccgctatt
     3481 ctacccatca tgcggcaaag cgcatgaggc gccaggctgc ggcttgcccg atattgcaag
     3541 gggaggcggg atgatcgaga cgctcaaggc gcgggtcaac ggcgacgagg cgctggtcag
     3601 gcgcgggcgg tttctcacga cgacgttcct tctggaagtc ggccagacct cctggctgat
     3661 ctcgatcttc gaaggcaagg tcttgtcggt gacgccaggg ccgttcgtga tgccgtcgtc
     3721 gtcattttcc ctgcgcgcgc cggaacagga atgggaaaaa ttctggagca gccggccgcc
     3781 gcccggctcc aacgatctga tggccctgat caagcgccgc gttctgaagg cggaaggcaa
     3841 tcttcaggtc ttcatggcca accttcgtta cttcaaggaa gcgcttgcga aattgcgtcc
     3901 tgagggagtc aaggcatgag cgcgcgattc gagccgatcg ttggccgcta catgcatctt
     3961 gaattgttcg ggcgcccaca tcgcatctat gtcgaggagg caggcgaggg cacgcccttg
     4021 ctctgcctgc acaccgccgg cgccgacggc cgtcaatacc gcgcgctgat gaacgatgcg
     4081 cgcatcacgc gcggccatcg cgtcatcgcc ttcgacatgc cctggcacgg caagtcgtcg
     4141 ccgccctcgg gctggcacaa cgaggaatat cagctcacct cggcgcacta cacgacgatg
     4201 atcctcaaga tcatgaccgc gctcgaactc gatcggccga tcctgatcgg atgctcgatc
     4261 ggcggccgca tcgcgctgca tctggcgctc gaacatccgc aacagttccg cgccatcatc
     4321 ggcctgcagg ccggcgccca tgtcgatccc tattacgatc tgaattttct gcaccgcccc
     4381 gacgtccatg gcggcgaagt ctgcgccgcc atcgtttccg gactggtcgg gcctgacgcg
     4441 cccgatgcgg agcgctggga gacgctgtgg cattacatgc agggtggtcc gggcgtcttc
     4501 aaaggcgatc tgtatttcta cacactcgac ggcgacatca gaggccgcgt cgcggagatc
     4561 gacaccagcc gctgcccact gtttctgctg tccggcgaat acgactattc ctgcaccccg
     4621 gaggaaacgc tcggcgttgc aagcagcatc caggggtgcg aagtcaccat catgaagggc
     4681 ctcgggcatt ttccgatgag cgagaacccg acggagtttc tcaaacacct gctgccggtg
     4741 ctggagaaga tcaaaggccg atgatttttg cggcggcggc gcagcgcccg aaacggagct
     4801 gcccggcggc cttcatgtgc ccgttgaggc ggctctattt ccgcagtgga gggggcgcgg
     4861 aatcagtccc gatggcgacg cgcatcctgg cgatggtggc gcgacaatac gcctcccgct
     4921 cccgagtgaa gcgctgctga ttggcgcgaa agttggcgac ccggttctgt atttcggttc
     4981 ggacttcgcc agaaacagtt ggccgcggcg cctggatgtt gaatggggtg ctggccggcg
     5041 gttcgaccaa cttgatcgcg tcgatcggcg cggcctcaac gcgaggcttt ctgtcgggct
     5101 ccgcgcccgg tttccacgcg cccggtttcc tggcgtctcg caacttgtcg tctcgttccc
     5161 ggtcggagac cgattgaacg aacgccaacg tttgcgcgat caaaagctcg cgctctctta
     5221 cccattccat cacacacctc gcccgtgaac actccaacaa gaaccgttgc gccaaagcaa
     5281 gcgacgtgag gtgtgcggta gctgaacgct gtggataacg tgggctcacg tcgactccga
     5341 cgataggcct cagtcttgtc cgcgacggcg atggtcgcga gatatgcggc gagctgctcg
     5401 gcttcgagcg tggtgccggt tgagaggatt gaacccccga cgttcggaac gctcagaaca
     5461 cttcaaagcc gtgggttccg tcgcgagcga gctgctcgac gaggccgaat tcccaatcga
     5521 gataggcctg catcgcggcc gccgcgttgt cggtgccctc atagggccgc ttgtagacgt
     5581 cgtcgaccgc gtcggcggcg cgagtcagcc cgcgctcgat cggcaatccg gccgcctccc
     5641 acgcccgggt tcctccggcc agaatggcga ccggtcgatc ggggaggacg gcgagatcgg
     5701 cggcggcgta acgcgccagg gtgccatcgc aggatgtcag caccggccgg cggccggcgg
     5761 gcaatcggtg cagcgcctcg gcgagccgcg cgcgaacgac gaaccaggcg ccaggcacat
     5821 gcccggcgat atggcgcggg ctggtggcca ggtccaccac gaccgtctca tcgagctccg
     5881 ccgccagcgc ctcgggcgcg agcggcaaca cgtcagccgc cgggaagggc gggcggcgag
     5941 gcgggcgcgg gccgatttcg agctcatcgc cggcgatgcc gttgtccaga acatggacgt
     6001 ttcgccaacc catctggatc agccaggaag cggccagcat tgcgcgcacg ccgtcgtcgt
     6061 cggccaggac gaggtgggcg tgacgcacgc ccacccattc gtcggtcgcc tgcaccaatt
     6121 gcccgcccgg cgccgagcgg aagcccggca gatgaccggc ttcgaattcc tcgggattgc
     6181 gtacgtcaag caggaacagc gtgaaccccc tcggttccct ccgccatcct tcgaccgtat
     6241 cccggccgac cgagcgaatc cccgctcgtt ccgcaagcgc ccgcgcgcgg ttcagcgctg
     6301 tcgcacgcgc ctcggccgag agttttggaa accgccggcc ggcgccgcga tcgaggtcca
     6361 gtccggcgag cgtccagccg atggtgccgt tgcgcagggc gaaaaccgga ttgggaatcc
     6421 ccgcattgat caatgactgg gtgccgatga tcgagcgggt ccgcccggcg cagttgacga
     6481 tcaccgtcgt ctccggccga ggcgccagat cttgcagtcg caacaccagt tcgccgccgg
     6541 gcacgctggt gccggtggga atcgacatat tgcgatactc ctcgaaacgg cgggaatcga
     6601 gcacgacgac gtcggctttc tccgcgatca accgatcgac ttccctggcg gagagcgatg
     6661 gcgtatgccg cttcgactcc accaattccc caaaggcctt gctcggcacg ttgacgtcga
     6721 tgaacacctc accaccggcg tgccgccatc cgtcgaggcc gccatcgagc cgcgcgacgt
     6781 cggtgtagcc gagcgcgcgc agacgggcct ccgcgcgctc caccagtccg ccgccgtcat
     6841 cgtagagaac gatcggtgcg tcgaagcgcg ggacgagatc gagcacctta agctctaacc
     6901 ggctgagcgg cagcgacacc gcgaacagcg gatgaccgcg cgagtagggc ccttcttcgc
     6961 gcacgtcgag caacgcgatc tcgcgccctt cgataagggc ggcgcgaacg gccgcggtgg
     7021 agacaatgcg ggtcatggca gatcttcctt gtgggagctg acaggctcgc gccggtgggc
     7081 cggctcagcc cgcggagcga tcccaaggct tggcgacggc gagtgcaggg agttcatgct
     7141 gtgcacgaaa tggatatggc agggcgttct ctggatcgcc acttcggcga tgtctgcatt
     7201 ttcgcatgaa ggagagggac gaaccagttt tgtttgcgca accgtgttca attaacggcg
     7261 tacgcatggc ccgattcgag cgcggccaca gagttatcac agtgcggcga tcgtgatcgt
     7321 tctcgagaac agccttgctg cggacatcgg gaaccgtgag agaacattct cgttgacacc
     7381 ctgtaaaaag cgtcagaacg ataactcggg aggttgctgc ggtttcgcgt ttcaagggac
     7441 cggcaaaacg gggctgggcc tgcatcttcc gttgttccgg ttcgaagggt tcgatgaagg
     7501 gttcggtaag ttcgaagaga ctgggcttct tcacgcggct tcttgatgat gctgctccag
     7561 cggaccgata tcgcctggcc gcagcgcaga tcaggcacgc ggaaagcgca ggctttgatt
     7621 ccgcatggat tgctcagcac cattttcacg aaggcgaggg cggtcttcct gcgcctttcg
     7681 cctttctcgg tttcgttgct gcgcagacct gtcgcattcg cctcgggacc ggcatcgtca
     7741 cgctgccgct ggaaaatccc gtgagggtgg cggaggacgc cgcggtgctc gatttgctgt
     7801 cgaacggccg cctcgaactc ggtgtcggga ccggcggaaa cccgtcggct ttcgcagcct
     7861 ttggcctgga cagcgccgag cgcgcggaaa ttttcgcgcg caatcttgat atcgtccgca
     7921 acgccctggc gggcgggccg ctcgccggtg gcgatacgct ctatccaccg aggccgcaat
     7981 tgtgcgaccg gatctggcaa gcgacttttt cagtgagtgg cggaacgcga gctggcaaag
     8041 cgggcgacgg cttgctgttg tctcggaccc aacctcgatc cggagacgcc ccgcgggcgt
     8101 cgctggcaga gattcagcat cccattattg acgcttacct cgacgcgctg ccgccgggac
     8161 gcgagccgcg aatcatggcg tcgcgtagcg tctttgccgc cgatgatcgt caggaagcat
     8221 tccgcctggc cgacatcgga ctgcggcgcg cccgcgatca tttcgtcgct tccggacacg
     8281 cctcgccggg agacgcgctg accgatatga tcgctgcgtt cgacgttcat gtgggaaccc
     8341 ctgaagatgt catcgtgtcg ctccatgccg acagcacgct gtcgcgggtg accgacctcg
     8401 tctttcaggc gcattcgatc gatccgccgc atcgatacat tctgcgatcc atcgagttaa
     8461 tcgccgaaac ggttgcaccc gcattgggct gggcaaaacg gacgcgggcc gatgcggcgc
     8521 tggtcgagta acaagacgtg gcagaaagaa gccgaaccat gagtacagag ccaaaagatg
     8581 tcatcgatct cctcgccggt atcgaacccg gatccagcct cgacgggatc cgcgcccggc
     8641 gcttgcaggc gcgggagaat gctcaaaaga gctatcttgc cttgttcgag cccgccgata
     8701 ttggcgatgt cgcggtcgcg gaacgatatg ctgttgcggc tttcgtggct ggcatccatg
     8761 ccgagccggc cgcagccagc ttctactcgg ccgggtttgc aagatcggcg gatcgatcgg
     8821 aactggtcga tgtgctcgga gccgaaatcg agcgagcaag gacggaaggc ccgtacggcg
     8881 cttttcccgc cgggctgcta tcggtcgagg tcaagggtgg gttgatccat cgcattgccg
     8941 gaaaaagccg gagtgtcctc ggcggacgat tggccgcggc ccttgagcac gcccatttgc
     9001 tggtcttcca tccccgtgat gccaatccgg cggccatccg ggtgctgctg gaagccggtt
     9061 ggtcaaacac ggcgatcgtg acgttgtcgc aactcgttgc gttcctgtgt tttcagattc
     9121 gaacgatcgc gggtctccgc acgcttggcg cgtcactcgg gcacggcgcc agcgtttcag
     9181 ccaaagccag ttgagggagc gccaggatca tgtcggagtc aatcgtcgtt cgcgccagca
     9241 aggatcagcc cgtgacgttc acccaggagg agttgggctg gctgccctgg cttgatccgc
     9301 ttcccgaagc cgagtttacc gagcggcatt gggccggact ggtcgagcga tcccgcgcaa
     9361 agtcggatta cttccggctg ctggtgcgcg atccccagat tctcgaagcc agaaccaaga
     9421 ccgacaagga catcttctac aacatcgcgg acggcttgcc gcgggcggag cgcgaactcg
     9481 cggccgcggc aacgtcccgc tacaatggat gcatctattg cacgtccgtg catgcgcgtt
     9541 tcgctaccgt ccactccaag cgccgcgatg acattcagcg gctgctcgac caaggcgtgg
     9601 gcgccgatct cggggagcgc tggaacgcga ttgtcagcgc ctcggttgcg cttgcctcga
     9661 cgcccagcgc ctttggtcaa gagcatattg aacaattgcg cggcgtgggt ctcaatgacg
     9721 ccgaaatcgt cgacgtgatc aacggtgcgg ccttcttcaa ttgggccaac cggctgatgc
     9781 tctcgctcgg ggagccctca acggtggtcg acgcatgacc gcatcggccc ctcgtcgatt
     9841 gtcgtttttc cgttagtccc gccatccgga atgcaaaaca tggctttcgc tctcgaccat
     9901 gtcgttattg ctgtcaacga cctcgatcgc gccgtcgcgg actatcaatc gctcgggttc
     9961 acggtgtatc caggcggcgt ccaccatggc ggcgtgtcgc ataatgcgct catcgtgttt
    10021 gaagacggct cgtatttcga gatcattgcc tatcgggaag cggcgccggc caaccgctgg
    10081 tggcatgtcc tgaccacagc cggtgaaggc atcgtcgact tcgcggtctt gccggaaaac
    10141 actgaaaaag atctcggggc ggcgcgggcg cggggtttga atctcgaggg gccgacgcca
    10201 ggcggccggc ttcggctgga cggtgttcgg ctcgactggc agatcgtgcg tccagcgaca
    10261 accgacctgc cgttctggtg cggcgacgtc accccgcgcc atttgcgcgt ccccgagggc
    10321 gccatccgcc atcacgccaa cggggtcacc ggtatttcgc gggtgcggat tttggtctct
    10381 gacgccgtga cgagtaccga gcgttacagg gcgctcgtcg gtccagatgc ggtcagtctg
    10441 gaaaacggca tcgcccggat aaatctcgaa agcacggtgt ttgagttggt ggtgcccgac
    10501 aatgacccgg cccgcggtca cctcaattca cgcggggagg gcgtgtggtc gctcgaactg
    10561 cgcggcgtgg cggcgaaagc gctcgatccg aagcggctgc atggcgccga ccttgccatc
    10621 gtcgggtgag cgttgtaacc gaggcttcaa cccgcggtcg ccaggtatga cccggccgct
    10681 tgtggcaccc agcgctggcc cggaatcgat cggagcagca gtcgcgtgta gtcatgttgg
    10741 ggcgaggcga aaacctcggc tgttgcgccg tgttcgacga tttcgccgtc cttcatgacg
    10801 gcaacgcggt cgcaaatctg ggccgccacc cgcaaatcgt gggtaatgaa gatcatcgac
    10861 aggccaaacg tcctccgcag gttggccagc agccggagga tttgcgcctg cacggagaca
    10921 tcgagcgccg aaaccggttc gtccgcgacc agcacgtcgg gacgcagcgc cagcgcgcgc
    10981 gcaagcccga tacgctgacg ctgcccaccg gaaaattcat gcggatagcg gtccatggct
    11041 tgcggatcga ggcccaccac gccaaacatt tgctttgctt ccgcccgtgc ctgtgggccg
    11101 ggcatgccat gaaggatcaa tccctgcgtc accagttcgc ccgcggtccg cctcggattg
    11161 agggagccgt aagggtcctg gaaaaccatc tggatgtgac gcgattgcgc tcgcaaatcg
    11221 cgcctggaca tggatgtcag ctcggcgtcg ccgagccgga tcacgccacg atccggatca
    11281 atcaggcgta cgatgcagcg cgccagcgtc gattttccgg aaccgctttc gccgacgatc
    11341 ccgagtgtgg tgcccttggt cagcgacgcg ctgacgtcct tgagtgcctg ggtgacgcgg
    11401 cggctgcgcc gcagcagccc aaactgccga taggtcttgg atacatgcga gatcgtcagg
    11461 attttttcgc catcggcggc actcgaaggc ggcggcagat acgacggaac cgcggcgatc
    11521 agctcctggg tgtagggctc ggacggatga ttcagcaatt ggtccctggc gccgtgctcg
    11581 acgacacggc catgctgcag caccgtcacg cgatccgcga tctcggcgac gacgccgaaa
    11641 tcatgggtga taaacaggac ggaggtgcct ttgcgcgcct gcaggtccct gatcaaggcc
    11701 aggatctgtg cctgcgtggt gacatcgagc gccgtggtcg gctcgtcggc aaccagaatg
    11761 gccggctcga gcgccagcgc catcgcgatc atcgcgcgtt gccgctggcc gccggacagc
    11821 tcgtgcggat aggcgcgttc aatttcttcg ggttgcgcga tctggacatc cgaaagcaag
    11881 gacagcacac gcgtccggat cgtcgcgctg tcgaggtcgg tgtgggtcca aaacatctcg
    11941 ccgatctggt cgccaatcga gcgcaacggg ttaagcgcgg tcatcggctc ctgaaagatc
    12001 atgccgatgt gcgcgcccct gatcttgcgc atctcctgct caccgagcgt gagcagcgaa
    12061 atctcctcga accggatatc tccggcggta acccggacat tttccggcaa cagacccagg
    12121 atcgcattcg cgatcatcga ctttccggat ccgctttcgc cgatcaggca gtggatttct
    12181 ccgcggcgca gatcgatgct gacatcgtcg agcgcaaacg gccggtcggc tccgggtgga
    12241 agcgaaaccg tcaagtttgc gatggcaagc agtccggtca tctgcgcttc agccgcgggt
    12301 taagcgcgtc gttcaatccc tgtccgatca gcgacaccgc cagaactgtc atcaggattg
    12361 cgacaccggg aatggcggac acgtaccatt gggtgcgcag caccgcccgg ccgccgccga
    12421 tcaggttacc ccaggacggg acgttcgggt ccgagagatt gagaaaggca agcgcgctct
    12481 cgagcaggat ggcgacggcc atgacgacac tggcatagac gatcaccggc ggcatcgcgt
    12541 tcggcaatat ctcgcgaaag atgatctgca tatcgcgcat gccgatggtg cggcatgcct
    12601 cgacgaattc gcgattgcga agtgagagaa attcagcccg tgtcagccgg gccgaggccg
    12661 gccaggatac caatccgatc gcgatcgtaa cggttttgat atcggagcca aagaccgcga
    12721 ccagcacaag caacagtaaa aaattcggca gcgtctgaaa ggcttcggtc aggcgcatca
    12781 gcatttcgtc ggctattccg ccgtagtaac cggccagcgc cccgatcaaa atgccgatcg
    12841 tcatcgcgat cagcgtcgcg accacgccga tcagcaggga aacccgcgcc ccgtaaaaca
    12901 tcaccgccgc gatgtcgcgg ccctgcacat cggtgccgag ccagaaccgc ggattgctga
    12961 gcggccattg cagcggccgc cctgcgagat ctagcggatt gttgggaaac aggcttgccg
    13021 cgctcagcgc catggcggtc accaccacga gcaggccgag cccgatcagc gccacgggat
    13081 tccccaggaa tcgggtgagc gtctgcatcg tcgtcaattc gcgatgcgcg ggtccagacg
    13141 cgcgtagaga atgtccacca ggaagttgac gcagatgacg agaagcgccg aaacaaacac
    13201 gataccaagc agcgtattga ggtcgcgctg cacgacggcc tcataggcca gacgtccaag
    13261 gccgggcagc gcaaagacgc tttccacgac aacggagcct cccagcatgg tgccagcctg
    13321 caggccgacc agcgtgacca tcggaagcag tgcgttgcgc agcacatgcc gccgcagcac
    13381 cgctgccttg gccagtccct tggcgcgcgc cgtccgcacg aaatccagtg tcatcacctc
    13441 aagcatcgag gcacgcatga tccgaaggta agtcgccagg aaaatcaggc caagcgtcgc
    13501 cgtcggcagc gccagatgcc agacaatatc gacggcgcgg cgaaggccgg tgtaaccggc
    13561 gccaatctct tccaggccgc cggccggcag cacgcgaaga ttgacggcga agatgatgat
    13621 gcccatcagc ccaagccaga acgccggggt tgcgtagaag atcgcgccga tcgtcgaaat
    13681 caccccgtcc ggccattggt tgacgcgacg cgcggccagc acgcccagca caagtcctcc
    13741 cgcaaacgcg aagctcagcg acgatgccat caacagcagc gtcgccgaga gacgttcgag
    13801 aatgacgtcg atcaccggct tgccgtagat cgcggaaaac ccgaggtcca gttgaaccag
    13861 cctcgtcaaa tagcgaataa gctgaaccgt cgcggttgcg tccaggccat aatacttgcg
    13921 cagctgttcc atcatcgcgc tgtcgccgcc gcccatctgc gccacgaggg catcgaccgt
    13981 gtcgcccggt gcgaattgga gcagcatgaa caagccggcc agcacgatca gcagcgttgg
    14041 cacgctcgcc gcgagccgac gaattgcgaa caggaagaaa cgcatgacgg acggcccgac
    14101 ctctgtggac gttctggtct agagcctttt ccgtttcgat aggatcgaaa cggggctcta
    14161 gcttcttgat ttgacgcgtt ttcttgacgc gaaccggttt ccacttcgct tgaaaacgcg
    14221 ctggaccatc gccaaaagga atgaaacggc gttcctgatc agatggccgc cgtcaactga
    14281 tccggtcaac tgatccatgt gtcatgccag tcgccggaat cccatcgtgg tgaggtatgg
    14341 tgattctgca cttgcttgct ggtggccgcg ataaattggc gttccgtgac catccagatc
    14401 ggcagctcct cgttgatgac cttgacccag tccgcgtaca acaacttgcg tttaccggta
    14461 tcaagttcgg aggcagcttc gtcgatcaac ttgtcgaccg catcggactg ccagccccat
    14521 tggttggtcc acggcgctcc cttcggcgag cccgaccgat accagaccgt ggtggaaacg
    14581 gcaggatcgc cgcgatattg gtgccagccg gtggcgagat cgaaattcca gtcgcggtac
    14641 accgcggaaa gcgcgccggc gatgtcgaga ttgacgatat cgatcttgat tccgatcttg
    14701 ctcagcgatt gctggatata ggtggcgagc agcggcacgt cctcgccgtt ctggatcggc
    14761 acgatcttca atgcgaagcg aattccgtcc gcgccgcgct tgtatccggc ttcgtccagc
    14821 agcgcctcgg ccttcttgag atcgtgggca tagggtacct cgccgccggg atagaaggcg
    14881 ggtgaggagg aggggatcgg tccggtcgcc ggcttgcccc gtccatacag gaagttttcg
    14941 atgaagaacg gaacgtcgat ggcatggacg atggcacggc gaacccgcac gtcggacaat
    15001 tccttccggc gcacgttgaa ctccagcgta ttgttgaaag cgttcgcttc gatgccgcgg
    15061 cttgaaatgt cgaagcgtgc atccttcttc aggcgatcgt agtcggcgag cgacagcccg
    15121 gagtaggtcg agagctggat ctgcccggtt tccaatgcgg ccgaggccgc cgacttgtcg
    15181 gcgatcacgc gccacacgat cctgtcgaga tacgggaatt ccttgcgcca gtagttcggg
    15241 tttcgttcgg cgataatgaa ttgccctcgt tcatactgca cgaacttgaa gggcccggtt
    15301 ccaatcggcg cggtgttggc gggattttcg agaatgttgg tgccttcgaa cacatggcgc
    15361 gggacgatgt atccgagatc ggccagggct ctcagcagca gcggcagcgg catcggccgg
    15421 gaatagcggc acaccgcggt atggtcgtcg ggcgtatcga cggcctcgag gtactgctgc
    15481 agttgtgtgc cgtaattcaa atatttcttc caaagctcca gcgcggtgta ttgcacgtcg
    15541 gcggatgtga agggtttgcc atcgtgccag ttgacgcctt cgcgcaattt gaaggtgacg
    15601 gtcttgccat ccggcgccgc ttcccacgac gttgccagca ctggcgtcgg tttcccgtcc
    15661 gcaccgagat caacaagcga ttccacgatc ttggaagtaa cgatgtagac gccggttgat
    15721 gcgcgtatcg ccggattgag gatacgctgc tcgccaggga gatgaacact cagggtgccg
    15781 ccacgtttcg gctgctcctg ggcccatgaa acatccgggc gcgtagcggc gtaagctgcg
    15841 gcggcggtgg aaacgagaag gtcgcgacgg gtcaggcgca tgaaagtctg cctcttggaa
    15901 ttgccatcgg acgacaatcc atcaacagcc gacgaaaaaa caggctaatg tttagtcaga
    15961 accgctaggc cgagcaaaaa agtcattctg tacggccgcc gcaggttcgg aacgaagatt
    16021 cttccatttc gggcttccgg gacgatgacg acgccgcgac ggtggccgaa tccggcagac
    16081 tcgccgaagt ccgcgcgaat atcctattgt gaccgttcgg cgcgtggcga ttgcatgatc
    16141 gagccatcac ggaacgatct ggtccgtcta cggagaaatg tgcggcgtcg tgttgggctg
    16201 cggcgctccc tccggttcgc tttcgactgg aagcttctcg tcgtggaccg caaggcgcgt
    16261 tccgatccag aagggatcga tgacgtggtc gcggcgcgga tttgccgtgt tgggatgaat
    16321 gaggatgctc aggccgccat ggttgagcat gagccacggc accaatgtcg cgaagacatc
    16381 gttggcaaag gcgacctgga acatcgccgc gtcgtgagga ccgatccgcg catcatgcca
    16441 gcggccaagc tgcaccggaa agcgctcgcc gatccaggcg cgtagctgct cggcagtgga
    16501 tcgcgtggcc gccggatcat aatagacgtg ggcgtgatag cttgcgatct cggcgatcgg
    16561 cctcggagcg gccacggctg ttacatcact catcgatgtg ccatccttcg gtttgcgacg
    16621 gatccagtcc gttgacggcg aaaggggaga gcgaaacggg tctggtttct cgccggcgaa
    16681 gcatgaataa atcacaaaaa gcccttggcc ggaagcggtg cgcgttcgtt gcttcgacct
    16741 caaacaaaag ccccggccgt gccggggctt ttccgtccgt cggagcggaa cgcgatcagt
    16801 acttcccgaa cgtcccgccc gggccgaaca tcccgcttgg gccgaacttg tagttcactc
    16861 ggaccagacc gatgtcgatg tcctggttga tgcgatgggt catggtggtg agaccggcgg
    16921 cggtgacaaa gttgagattc ttgttgccca tgaacaggtg atcgtattcg acgccgactg
    16981 accagttcgg agcaaaggca tattccaaac ctgcgccgac cgtaccgccc cagcgcgtgg
    17041 tactggcgcg gtcgaccatc acgccggtcg cggtcgtgaa cgtgtcgtag cggtcgccga
    17101 cgacggcggc gccgcccttg aggtaggcca ggaaactggg gctccagcta tagccgacct
    17161 gaccggtgaa gagaccaaaa ctgttgacgc gggtacgatc agtgagcgtc ggcagaatca
    17221 ggttgcgact attgcccttg aagctcgacc aatcgccctg cgcttcaacg ccgaacaccc
    17281 agttcgacac ctggtagcga taaccgatct ggccgccgac cgcggcgcca ctggcgttcg
    17341 tggtaccctc gttcgagagc gctggattca cggcaacgcc gccgttcgtg aggagattcc
    17401 atctgtcgcg gctggagccg ccgccggcgt tgaaaccgac atagaagccg ctccagtcgt
    17461 tgattgtagc gaccattgcg ggcggcgcct tggtataggg gcgtgccgga agatcggcag
    17521 cgaaagcggg cgccgatgcg acgagtgcaa gaaatcccgt cgaagcgagg agaagctttt
    17581 tcatttttga attccgtgcc agttctttat ggagagtccc cagggccttt tggtggcccc
    17641 tcgaatagac ttcgactgcc tcccggcagc gaatagcaaa gcgtctttcg cagaacgatc
    17701 gttgcgtgag cgaaagttaa tcgcgtcttt cgggcattta tgggactctt ctaatctcac
    17761 gatttgctac gtttgctaac gatgtggaac caggatttgc cgatatatgg acaaatcgtg
    17821 gctcctgccc acgaccagcc acaatgcatc aggagattct tgatgcgtga gttgctgccg
    17881 ctttcggctg atgcccgcac acgtcgctat catcgcgatg cgcggcacca tcggatcgct
    17941 tccactcttc ggatgtagtc tgttttaagc aggcggatcg tgtcggtttc cgaatgcgat
    18001 gcaatccaag ccgtcaaagc atccgcctca agatgataac gacaggaagc atcgcctcat
    18061 cctcattcga caccggcgcg actgttgcgt tgatcggcaa cgcggtaagc tatgttggcc
    18121 cgatgatcgc gcgtccgctt ttcaaagcgc aatgccgacg agagcccagg gatttttttt
    18181 cagtgatagg agtattcgtg tgaaggctgt ctataccgaa ctgcatcgaa gccacgaccc
    18241 gcaattcttt ctggttcgcg gcgtcgtcaa gcgcaccacc gagcagcccg aacgcgctga
    18301 ccgtctgctc gcgggactca gggcgggcaa gcacgatctg gttacgccga gcgcgttcgg
    18361 tcagggaccg cgtgcaagag tgcacagtcc tgaatatctc ggttttctcg ccgaggcatg
    18421 ggacgcctgg accgagcttg gggattgcgg ccccgagatg atcgccaaca tgcatcccgt
    18481 ccgcaacgcc gcgacttacc cgacgcacat tgtcggccgc ctcggctggc atacgatcga
    18541 tacgtcctgt ccgatcggcc ccggcacctg ggctgccgct tgcgctgcca cggatgtggc
    18601 gacgacggcg gctcaactcg tcttcgacgg cgaggatgcg gcttacgcac tctgccgtcc
    18661 tccgggccat cacgcctacc gcgacctcgc cagcggcttt tgtttcctca acaacagcgc
    18721 gatcgcagcc gcccatctgc gaggcaggca tgagcgcgtg gcgatcctcg acgtcgacgt
    18781 ccatcatggt aacggtacgc aaggcatctt ctacgagcga cccgacgttc tgacggtttc
    18841 gatccatgcc gatccagcct tcttcaaccc gtttgtttgg ggctatgcgc acgagcgcgg
    18901 cgccgggccc gggcttggtg ccaatctcaa ccttccgctg ccgatcggca ccggggacga
    18961 cggctacacg caggcgctcg gcgccgccga aaagacgatc cgtgcttttg cgcctggcgc
    19021 actggtcgtg gcgctcggcc tcgatgcgtc cgagcacgac cctctcgcgg gtcttgcggt
    19081 gaccaccgac ggattccggc gcattggcgg cgccatcgcg cggttggggc ttccaactgt
    19141 tttcgtccag gaaggcggat acctgtcaga gattctcggg accaatctga cagctgccct
    19201 cgccggattt gaagaggttc gctagctgga gggcaggcgc agcaccgtgt cggcaacgga
    19261 gcatctatca caacgggatc gcggcttgat catcgcgcct ccgcacacca gcggtgacaa
    19321 tctgctcgac ctgatctcca tcgagccgac ctgtctcgat cagggcggcc gccagcatcg
    19381 agactgcggg ccagtgcgcc agtacgactg cgagcgcgcg ttgctcgaaa cgcgggaagg
    19441 cctgtcgtcg cttggtgagc gcacagagat cggcgataac ctttccggcg tggctaaaat
    19501 cctcactgct gccgcagtta aacaacgcga acgcgccgac atgcttcttg cgacgctcgc
    19561 cgcgagccac cgcttcggct atcggcccgg cgagatcaat cacgatctgc cattccatct
    19621 tgcggcgatt ctgcagagcg atgcgcgcta tgggccaggc cggagagtag atatcgggac
    19681 cgcggcaaga accgctccat tgaccagcag gcatctcgcc ttcgctgatc tcaacgtact
    19741 tgattgcacg tcgtcccgac ccgatagcgc ggaacgccag cacggatgcg agagcgtgcc
    19801 cggcctcatg gaaggccacg gtcgccgttg ccgttggctc ctgcggtttc ggtttgcgcg
    19861 atctcatggc ccgctcggaa tttctcgatg caggaaattg gacgtcaatg cgtcgactat
    19921 agaaggacga ccagccgacg tatcccaatt ccgagtagcc agatccaagc aaccatcgac
    19981 gcgatggcgg cggctaaaaa ggtcgccttt atgataggtt cgcgcatgtt acgctgccgc
    20041 ggcgtcagct gccttccgag tgttggctcg gaggatgcgg ttggaggaat atttcgacgg
    20101 tcattgaacc gcacggcgaa cactcggatg cgcggatatc gccgaaaaat tcgcgtgaga
    20161 ggtccttgag cctgcagcat gagcgcgtca cgctgtgggc aatttggtat atccggtttc
    20221 atgcttcgtc cccgcgcgaa gaaaattcgg agattgcaac gcacatatca ggaatgcgtt
    20281 cccgtcattc gcgtccgttg aatgccagcg actgatgcag gtctgttcgg caacggactc
    20341 aggaaccgac ttttgcgcgg tcctgttgcc gcgttcgagt gctgcacgat accgggagtt
    20401 gcggtggccc gtgccagatg cttcttggtt ggccgtgaat tgcatatttg cggggcggac
    20461 tgcggagatt ttgatcgacg gttttgttcg gccggtttga ccgcgaaatt accgcgaatc
    20521 ccaccatcca agtcccacca taaagaaacg gcagactgag catgtttgcc gagcgcgctg
    20581 ccctcaaatc gcgaggaacc gcattcgtgc catgacgttc taacagcgtg gtattggaca
    20641 attctttcgg tgcggcatgg cttggggtct tttgagcagg tcggacaagc ggagtgctgt
    20701 ctcgcgcaag cgtttggagt cggcagtcac tgaggccgtg aagtccgcgt tggattgcga
    20761 ggcttttgtt ggtgtgatta tccaacggat agcaccgaaa tcgcgtaccg acccaaattg
    20821 ggctgtcagg ggtgtgcggt tcggcaaagc tgatcgggat aaatcgggcg aggtgctggc
    20881 aactgttgtc gaacagatgc agcgcgaatt tagcctaacc gacaatcaag acgacgcgcc
    20941 gcaaacgaaa gatgcaaaac cggaagtgat acaaatttgt cgcgatgagg aacgcgcttc
    21001 tgccgccagt gggtatgatt agatcggccg gcattcctgg cagcgagcgg cgatgatgga
    21061 tggggagggg gagccatgtc acttcccgcg cttataggct ccttgcatca tctcccatcg
    21121 gctcatcagt ctcatcggtt acaaggggcg cctgcaatct gccggcctct gcataaagca
    21181 gttgctacag ttgatgagga tgcatcgtta gtttgggcca cagggctcct ggaaaaacgc
    21241 ggatgaagaa tccagcgaga aagaaagcga aggctcgttt tgaggccgcg cttgatagcg
    21301 ccgtgaacat gctgcccaag tcgccgaaag agaagaaggc gccggcgcgg gaaaaatcta
    21361 cgccgtcaaa taatcgtccg acacgcggaa gacgcccttg atgcacgact tttctcgaac
    21421 gcaaatttga atgtgggtct tttcgaagaa gcccgcttcc tcgtagatcc tgccgccttc
    21481 gataaagact gccttgacgg tttgaaaggc cggtagtttg gccgcgtctc ttactttgtg
    21541 gagatggttt atcaccgcgc aatcaaggtc tctgaggagc ctgtcggggc caagccggtt
    21601 ttttggaacc ggcttgtccg cctttttggc gtaagcaatg aagtcttcat gggctgcctt
    21661 gacggcagcc acaccggtgg aagaaatcag atcaaggcaa aaccccaaat cgatcacggc
    21721 gcccacaacc gcaggtgccg tgattttgcc gcgcgaccgt cccgttttgg cgaactcaat
    21781 ccccctcacg ggattggttt cccaaaagta gatgcccgat ccgagccagt cccaatcatt
    21841 ggcgctggga acgaaatctt ttccgttcag gagatcatca gcgaccgatt ggtcgcaccc
    21901 gtgaaaaccg aggacaaacg aactggaaag ccggtgcaat tacgcagctg caacggccgc
    21961 ggagtatcgc ttggacactt cgccattttc gtccaggaat ccctcttcct tcagaaattg
    22021 agtggctttc tctcgggacg aggtgtgctc cagacggagc tttgccatcg cggccttgaa
    22081 ttcccgcagt tcctgctcgc tcatattgtc aactccacgg agcttgggca gaaggataat
    22141 agatagaatc tctaaaagtt caatgactta tgcgcgaacg ccccgccgcc atgcgccgcg
    22201 gggcgttcaa attgtctgag aaaacctcag ttcgccagcg gatcgggccg aaactggatg
    22261 tgcacggcgc gcggccttgc gcccttgccg ccttccttga gccagggcgt gcggtcgccg
    22321 ttcgtgctcc acaacccgcg gcctttccag ccggcgttcg gatcgtcgat acgcccatcg
    22381 aagcccttgg catagaagct gagcggatag ggaatccgga tcgtgatcat gttgccgtcc
    22441 ttcagcgcca cgaagccgtc accaagattg gcggtggaca tcgggatgtt ctcgcccagt
    22501 ccagacgtgt tgtgctggtc gacccaggtg taatagctcg actcggcgct gttctcgccg
    22561 acgccttcga aaccggggcc gggatactga tagatcgtcc agccctccgg gcaatgattg
    22621 ccggtggcct tcggcccatt gagcgggcct ttgcacttgc ggcggtcaaa gctgccgagg
    22681 tgaccactcg ataacgacgc ccagaccaca ccgttcttgt caatatcgcc gccgcggact
    22741 ccgaacgccg gcgcgggaac gttgtacact tccgacagtc cggtcaccgg gtcgaatcgc
    22801 aggacccccg gcgtgccacc gaagatgccg acggtgtacc agatc
//
LOCUS       DATNOI010000258         2525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_899570,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000258 DATNOI010000000
VERSION     DATNOI010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899570"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VN968_10030"
     CDS             complement(<1..492)
                     /locus_tag="VN968_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXL29611.1"
                     /translation="MSTYRLKNLLSPRSLALIGASPRRGSVGRAVLHNIRKAGFKGEF
                     GLVNTHYADIDGIAAVASLDSLPFVPELVVITAPAAAVAGIIDDAGKLGAAGALIIPA
                     GLGHGAGSLADAAERAAQKYGMRLIGPNCLGIMMPAANLNASFSAHMPVAGNLALISQ
                     SGAI"
     gene            complement(545..1366)
                     /locus_tag="VN968_10035"
     CDS             complement(545..1366)
                     /locus_tag="VN968_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXL29612.1"
                     /translation="MPLVGEPSAAAIAAIDKCMAVAGDMGARVTAMAVEEDILVRPKV
                     TISDDLDNTAAAEAVRSVSNAHGLLKASDAAATRFGVRNEQRLSRFAAADIAMNIAVW
                     ARLKDMSIVPIKPHDDQSEKIVERLIFESGRPILMCPEESASELAVAFDQVVIAWDHT
                     APAARAVADALPMLQAAASVRIVTATDNNTPAELESGAALVSHLAEHGIKATFETVKI
                     DGSAVGKVFEAYVKANAIDLLIMGAYRHSRLNELVWGGATKTAIGRPPCWVIMSH"
     gene            1579..>2525
                     /locus_tag="VN968_10040"
     CDS             1579..>2525
                     /locus_tag="VN968_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HXL29613.1"
                     /translation="MHAMVLPAPGAPLQMQERPDPSPGDGEIRVKVSACGVCRTDLHV
                     ADAELPGIKYPIVPGHEIVGRVDLVGSNVTTHRIGDRVGIPWLGYTCGVCRFCKDGME
                     NLCDRPQFTGYMRDGGFATHTIADARYAFPLGEEGDDVSIAPLLCAGLIGWRSLVLAG
                     NAGRLGIYGFGAAGHIVAQIARWQGWSVYAFTRSDDTAAQEFARRLGAVWAGGSDQLP
                     EPPLDAAIIYAPAGQLVPAALRAVRKGGRVVCAGIHMSDIPSFPYDLLWEERQLVSVA
                     NLTRQDGLDFLKIAPQAGIRTQTTAFPLAEANEVLTKLRT"
BASE COUNT          483 a          800 c          743 g          499 t
ORIGIN      
        1 aatggcgccc gactgagaga tcagcgccag atttcccgca acaggcatgt gcgcagagaa
       61 actcgcattg aggtttgccg ccggcatcat gatgccgaga caattgggcc cgatcagcct
      121 catgccatat ttttgcgcgg cgcgttcggc agcgtccgcc agcgaccccg cgccatggcc
      181 aagcccggcg gggatgatca gggcgcccgc cgcgcccagc tttcctgcgt catcgatgat
      241 gcccgcgacg gcagcggccg gcgcagtaat caccacaagt tcgggtacga atggcaagct
      301 atcaaggctg gcgactgcgg cgatgccatc aatgtcggca taatgcgtat tgacgagtcc
      361 aaactcgccc ttgaatcccg ccttgcgaat attgtgaaga acggcccgcc cgacggagcc
      421 tcgccgcgga ctcgccccga tcagcgcgag tgaacgcggc gaaagcagat ttttcaaacg
      481 ataggtcgac atcagctcaa ttgcccccgg accgccgccc agtatgtaag cgaacttcgg
      541 gacgctaatg cgacatgata acccagcacg gtggccgacc gatggcggtt ttggtcgcac
      601 cgccccacac cagttcgttc agccgggagt gacggtaggc gcccatgatc agaaggtcga
      661 ttgcgttggc cttcacatac gcctcgaaca cctttccgac cgcgctgccg tcgatcttca
      721 ccgtttcaaa agtggccttg attccgtgct cggcgagatg gctcacgagt gctgccccgg
      781 attccagctc tgccggggta ttgttgtcgg ttgcggtgac gatgcgcacg ctggcagccg
      841 cctgcagcat tggcaatgcg tcggcaacag cccttgcggc cggcgcggta tgatcccacg
      901 cgatcacgac ttgatcgaat gccaccgcca attcgctggc ggattcctcc gggcacatca
      961 ggatcggcct gcccgattcg aatatcaacc gctcgacaat cttttccgat tggtcatcat
     1021 gtggcttgat cggcacgatc gacatatcct tgagccgcgc ccataccgca atattcatgg
     1081 caatatctgc cgcggcaaat cggctaagcc tttgctcgtt gcgcacgcca aagcgcgttg
     1141 ccgccgcgtc ggatgctttc aacaagccat gcgcgttcga cacgctccgc accgcctcgg
     1201 ccgccgccgt attatccaga tcgtccgaga tcgttacctt cggtctgacc agaatatcct
     1261 cttcaaccgc catcgcggta acccgggccc ccatatcacc agccaccgcc atgcatttgt
     1321 cgatcgcagc aatcgcggcc gcactcggct cgccgaccag cggcaacaga atatccttga
     1381 tcgccatgtg cgctctcctt tgatgagcga tagggtactc gcacgcaagc cgtgttgatc
     1441 ttcgtcaaga cgacaagccc gcttttgaca attcataggg tattcccgca aaatggattt
     1501 gattgaggtc aagggctcgc agggccccgg cggtatgcgt tatcctggcc accgtatcgg
     1561 ttgctcgcgg agaaacagat gcatgcgatg gtgttgccgg cgccgggggc gcctctccag
     1621 atgcaagaac gcccggaccc cagccccggc gacggagaaa ttcgcgtcaa ggtaagcgcg
     1681 tgtggggttt gccggactga ccttcatgtc gccgacgcgg agcttcccgg catcaaatac
     1741 ccgatcgttc cgggccacga aatcgtcggc cgcgtcgacc ttgtcggcag caacgtcacc
     1801 acacacagga tcggtgaccg ggtcggaatc ccctggctcg gatacacctg cggcgtctgt
     1861 cgtttctgca aggacggcat ggaaaatctc tgcgatcgtc cgcagtttac cggatatatg
     1921 cgggacggcg gcttcgccac gcacaccatc gccgacgcac gctacgcatt cccgctcggt
     1981 gaggaaggcg acgatgtttc gattgcgcca ctgttgtgcg ccggcctgat cggctggcga
     2041 tcgctggtgc tggcgggcaa tgccggacgg ctcggcatct atggcttcgg cgcagccggg
     2101 cacatcgttg cccagatcgc gcgctggcag ggctggtctg tttatgcttt tacgcgatct
     2161 gatgatactg cggcgcagga atttgcccgc cgactcggtg cggtgtgggc cggagggtcg
     2221 gaccagctgc ccgaacctcc gctcgatgcc gcgatcatct acgcccccgc cggacaattg
     2281 gtgccggcgg cactgcgggc ggtacgcaag ggcggcaggg tagtatgcgc gggaattcac
     2341 atgagtgata ttccgagctt tccctacgat cttctttggg aagaaagaca gctggtgtcg
     2401 gttgccaacc tcacccgcca ggacgggctg gatttcctta aaattgctcc tcaggcggga
     2461 atccggaccc agacgacggc attcccgctt gccgaggcta acgaggtgct tacaaaactg
     2521 cggac
//
LOCUS       DATNOI010000259        17912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1987086,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000259 DATNOI010000000
VERSION     DATNOI010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17912
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1987086"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /gene="aroC"
                     /locus_tag="VN968_10045"
     CDS             <1..999
                     /gene="aroC"
                     /locus_tag="VN968_10045"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXL29614.1"
                     /translation="EDIQRDLDRRRPGQSRFTTQRQELDAVKILSGVMAHPETGVQVT
                     TGTPIALLIENTDQRSKDYSEIKDKFRPGHADFTYEAKYGLRDYRGGGRSSARETAMR
                     VAAGAIARKIVPGMTVRGALVQMGPHQIDREKWDWDEIARNPFFCPDKDKAAFFESYL
                     DGIRKKGSSIGAVIEVVAEGVPAGLGAPIYAKLDSDLAAALMSINAVKGVEIGAGFGA
                     AELSGEENADEMRMGNQGVSFLSNHAGGILGGISTGQPVVARFAVKPTSSILSPRRTV
                     DRNGADTDIVTKGRHDPCVGIRAVPVGEAMMACVLADHFLRHRGQVGSAARTDNAI"
     gene            1064..2248
                     /locus_tag="VN968_10050"
     CDS             1064..2248
                     /locus_tag="VN968_10050"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL29615.1"
                     /translation="MNAAELQKITDWLIDGARSAPTPPQMMAEACERLVRAGLPLWRV
                     GVFVRTLHPDIFGRNFIWRPGAEVEVGTVDFAILDSPEFQSSPLAIVFREGIEVRARS
                     DDPESKRFPIVEDMRAEGVTDYIALPLLFMDRAVHASSWTTKQAGGFSEEQLNALRSV
                     IAPLARYIEIVSLRRTASLLLDTYVGNRAGERILGGQIRRGHTDTMDAAIWLSDLRGF
                     TALSDRLPAETVVDILNGYFDCQVSAIRTHGGEVLKFMGDGLLAVFPIDEYVGDTQQV
                     CSSVLEAARESRASVDVMHYPIGETVEHFRFGVALHVGRIRYGNIGGGNRLDFTCIGP
                     AVNLAARLEKIAGRLSRTIVASAGFAGICPDGWADLGEFPIAGFSKAERVYGLQDETP
                     AA"
     gene            complement(2275..2580)
                     /gene="clpS"
                     /locus_tag="VN968_10055"
     CDS             complement(2275..2580)
                     /gene="clpS"
                     /locus_tag="VN968_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HXL29616.1"
                     /translation="MIDAVTKPKTKVKTKTERPRLHKVILINDDFTPREFVVTVLKAE
                     FRMTEDQAHKVMITAHRLGVCVVAVFTKDVAETKATRATDAGRAKGYPLLFTTEPEE"
     gene            2907..3752
                     /locus_tag="VN968_10060"
     CDS             2907..3752
                     /locus_tag="VN968_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HXL29617.1"
                     /translation="MRWCVSIGALLVAGAMAGGDVAGIAAPNPTNALADKDAASSVDL
                     ELVLAVDVSYSMDMDELAIQREGYAQAIISKEFLQALKTGPTGKIAVTYFEWAASSDQ
                     KIIIPWRVIDGPETADAVANEIMKTPIRRASRTSISGAINFAMPLFDENPYHGLRRVI
                     DISGDGPNNNGAPVTGARDAALEKGITINGLPIMVKEPSYSTMDIDNLDLYYEDCVIG
                     GPGSFVVTIKDREKFKEAIRTKLLLEVADRTPERRTVPVAEKEKEPRVSCLIGEKIWQ
                     DRWGR"
     gene            complement(3892..5130)
                     /locus_tag="VN968_10065"
     CDS             complement(3892..5130)
                     /locus_tag="VN968_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXL29618.1"
                     /translation="MAERLVIDHVAHHGDGVAFADGAAVYVAYTLGGETVEVSAVPGH
                     PDRRRLLAVERASPERVTPFCPHFGVCGGCAIQHWESERYRAWKRDIVIETLAQAKLT
                     CEVDPLIDAHGLGRRRITLHARIGTHDVLKVGFAAAGSHDIIPIDRCPILAPGLNGAL
                     AAGWAIAEPLTSIGKPLDIQITATDNGLDVDVRGSGPLPAARIATLSRIAEQHRLARL
                     TRHGDLVLMRNSPTIAIGAAQLTLPPGSFLQATAAGEEALAALVSGHLTRARHIADLF
                     CGVGPFALRLAAKSRVSAFDSDAGAVAALQKAATSTPGLKPVKAEARDLFRRPLMPQE
                     LRDYEAIVFDPPRQGAQAQAVQLAASKIAVVIGVSCNVATFARDARILVDGGYKLERV
                     TPVDQFRHTPHVELMAKFTR"
     gene            complement(5123..5857)
                     /locus_tag="VN968_10070"
     CDS             complement(5123..5857)
                     /locus_tag="VN968_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HXL29619.1"
                     /translation="MSPARKRADTLLVERGLFESRARAQAAIEAGLVTANDKQVMKPS
                     ESIAADAVLQAQAAHPFVSRGGVKLAGALQQYPIDVEGHVCLDVGASTGGFTEVLLAN
                     GASLVFSIDVGRDQLHPSLRGHPNVVSMEQTDIRSLEGKRLPARPDVVVIDVSFISLK
                     AVLPVALSLAAAPMHLLALIKPQFEAPRKHSRRGIVRDGTVHQQICNDIAGFAASLGC
                     SDIQVFPSSIKGGDGNTEFFIGARRG"
     gene            complement(5881..6456)
                     /locus_tag="VN968_10075"
     CDS             complement(5881..6456)
                     /locus_tag="VN968_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HXL29620.1"
                     /translation="MTKVYLAGPDVFLPDAADIGRRKVALCAQHGLIGLYPLENAIDR
                     AASDASLQIFRGNEAMMIEADAIIANLTPFRGPGADAGTVYELGYMAGRGKLCLGYSN
                     DPSSYADRVGEFIRVISRDGRLVDASGLTVENFGLTDNLMMIHALDLHGCALMTPRQA
                     PADIWHDLTAFESCARMAAGRMASAPARASS"
     gene            complement(6654..8579)
                     /gene="dxs"
                     /locus_tag="VN968_10080"
     CDS             complement(6654..8579)
                     /gene="dxs"
                     /locus_tag="VN968_10080"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HXL29621.1"
                     /translation="MTVFSKTPLLDTIHTPDDLRRLKVEQVRQVADELRQETIDAVSV
                     TGGHFGAGLGVVELTTALHYIFDTPRDRLVWDVGHQAYPHKILTGRRDRIRTLRTGGG
                     LSGFTKRTESDYDPFGAAHSSTSISAALGMAVARDLSGGKNNVIAVIGDGAMSAGMAY
                     EAMNNAGAMNSRLIVILNDNDMSIAPPVGAMSAYLSRLYSGRTYRSLREAAKQINKRL
                     PKVLADRANRVEEYSRGFMVGGGTLFEELGFYYVGPIDGHNLDHLLPVLKNVRDMEDG
                     PILVHVVTQKGKGYPPAEASADKYHAVVKFDVATGAQAKAKPNAPAYQNVFGQTLVKE
                     AQKDDKIVGITAAMPSGTGIDIFCKAFPERTFDVGIAEQHAVTFAAGLATEGYKPFCA
                     IYSTFLQRGYDQVVHDVAIQGLPVRFAIDRAGLVGADGATHAGSFDNAYLGCLPGFVI
                     MAASDEAELVHMVATQVAINDRPSALRYPRGEGRGVEMPEAGIPLEIGKGRIIREGSK
                     IALLSFGTRLGECEKAADELAAHGLSTTIADARFMKPLDVELVLKLAREHEILLTIEE
                     GAIGGFGSHVMQTLAEHGMLDGGVRMRSMVLPDVFLDHDSPNAMYARAGLDAKGIVAK
                     VFETLGKDFKAETVKLA"
     gene            complement(8916..10214)
                     /locus_tag="VN968_10085"
     CDS             complement(8916..10214)
                     /locus_tag="VN968_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29622.1"
                     /translation="MRVQHRHIIYVQGYDPRGLAQYYRMFRTELRKFGRLYQLDATVS
                     RPQVAPHGETASWTIETEASDWQTRTRYDFLRWEDLIQRDLAAPIWRTVFHAIWIYWR
                     VVFSGTFARFWKANWRFATFITYPHFLLLIEAMCALGIAFAFARGLEALGIPDPFSMA
                     AAAALFLAILGTVLKLSENATYLLYLLADTIWTWEFSHRKRPEWDERIDRFARYLIDV
                     AREGEADEIVVVGHSSGSFLGAEIMARALKLDPNLGQHGPRVVLLTIGGNFPIVGFHA
                     ASQDFRDHLRLLAVEPSIDWIDCQSRKDVMNFFPFDPIAGHSIEVGAARRNPTIVPVR
                     FREIIQPGHYKLFRWKFFRVHFQFVMANELPHAYDFFMIVCGPIPLSERMARPEAALA
                     FATGDAAERECAWKMLETAVPGRGDAADLGELEPTARRRG"
     gene            complement(10367..10618)
                     /locus_tag="VN968_10090"
     CDS             complement(10367..10618)
                     /locus_tag="VN968_10090"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXL29623.1"
                     /translation="MAENTQMDVKKLSFERAIEELESIVKRLEEGKVPLEESVAIYER
                     GEALKRRCEELLRQAEARVDKITTDANGQPTGTEPLDVQ"
     gene            complement(10691..11620)
                     /locus_tag="VN968_10095"
     CDS             complement(10691..11620)
                     /locus_tag="VN968_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HXL29624.1"
                     /translation="MTTLLVSHRSSLDHQTPPGHPERPDRIRAIEQVLGESRFDRLVR
                     EQAPEGSLDLVRLCHNEHYIEQLQHIAPKSGMIYLDGDTSMSPGTWEAAMRGVGGAVA
                     ATDAVMSGTHDNAFVAMRPPGHHAETNRPMGFCLFDHAAIAARHAQRKYGIARAAVVD
                     FDVHHGNGTQDIFWSDPTVMYCSTHQMPLFPGTGARGERGEHDTIVNAPLASEDGSVQ
                     FRAAFENVILPQLRKFSPELIIVSAGFDAHYRDPLASLNLRADDFGWVTQKLMDVADA
                     SAGGRIVSVLEGGYDLQGLKESVAAHVTALMGV"
     gene            complement(11724..13394)
                     /locus_tag="VN968_10100"
     CDS             complement(11724..13394)
                     /locus_tag="VN968_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HXL29625.1"
                     /translation="MRHPLCPVLAALLLAFALEPCSRAEAQAAPVELRILAINDFHGY
                     LQPPPGGIRIADPNDRSKKIAVEAGGAEQMATLVKQLRAGASNTIFVAAGDLIGASPF
                     LSALFHDEPTIESLSMMGLEVASVGNHEFDEGKEELLRMQNGGCHPTDGCQGPHPFLG
                     AKFRYLAASTIEKNTGKTVFPPYAIREFEGIPVAFIGLTLKGTPNLVSPVGVAGLEFR
                     DEADTVNALVPELKARGVEAIVVLIHEGGFPTGDYDECPGISGPIVDIVRKFDHAVDV
                     VISGHTHQAYVCEIDGRLVTSGDKNGTLVTAIDVRLDPATRDIISARADNVIVRTAAY
                     ARDPEQTALIASYDKVAAPIANRPAGSITETLSRAPNDAGESPLGDIIADAQLAATRA
                     DKDGGAVIAFTNPGGVRTDIPKKADGTVSYADVFSSQPFRNQLVTLTLTGAQIKNALE
                     QQWLDPKRPRILQVSKGFNYTWDNARPYGDRVLADRMSLNGQPIDPAKGYRVTVNNYL
                     AVGGDGFTALKEGTGRQFGIYDVDALHGCFQANSPISPQPADRIVRLN"
     gene            complement(13592..14356)
                     /locus_tag="VN968_10105"
     CDS             complement(13592..14356)
                     /locus_tag="VN968_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXL29626.1"
                     /translation="MTGHLIVADEGAMRVIRLRRPEKKNAITLDMYRGMSDAIDTAQN
                     NPAIRCLIITGGSGVFSAGNDVDDFLKDATANAETPRASNMTKFLYSLAHNVKPIIAA
                     VDGVAIGIGTTMLFHCDFVLASTTATFSTPFIQLGLVPEAASSLLMPRTMGYQRAFAT
                     LVMGRKMSAEDARIAGFVNVVVSPGHTEAEARKVAREICALPAEAVATARKLLRPPPE
                     DLTRRIDQEGHLFSERMRSHEAVAAFKEFLSRRQKA"
     gene            14490..15437
                     /locus_tag="VN968_10110"
     CDS             14490..15437
                     /locus_tag="VN968_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HXL29627.1"
                     /translation="MQLTGIHHLTAISAKPRENLAFYAGLLGMRLVKKTVNQDDVSAY
                     HLFYADGRANPGTDLTFFDFPAAPERRGSNSICATSLRVAGEQSLGFWRDRLKQAGGH
                     AGDISEVDGRLTLPFEDPEGQRLVLVDDGGVGSASPWEKSAVPAEHQIRGLGPIMLSV
                     RDLARTEQVLTGVMNMRSVRDYAAPDARAQARVFAMGEGGPAAELHVIEQKDLPEARQ
                     GAGGVHHVAFRTPNDEQYHAWARRLNELGVRNSGEIDRFYFRSLYFREPNGILFEIAT
                     DGPGFATDEPMAALGEKLSLPPFLEPRRAQIEAGLKPLE"
     gene            15730..15969
                     /locus_tag="VN968_10115"
     CDS             15730..15969
                     /locus_tag="VN968_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29628.1"
                     /translation="MTKLRLLPAAVIAAVMLTTPVVAQEYHPNARQAVASAPDRAAPG
                     VRYVDGRLCYPAPRVGAFATQPWENETPCQPLPVY"
     gene            complement(16192..16644)
                     /locus_tag="VN968_10120"
     CDS             complement(16192..16644)
                     /locus_tag="VN968_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29629.1"
                     /translation="MNHAQPVSMLSRGWIAGRATLLAVTVLGLLAAPARSPAQDAGVS
                     GIPPGPANARGLNGSVNDPSGIGNAARVPAIPPPSISPAMPSTVSPPTAFRTLPVQRG
                     VKIKRTRYAAARSRRGAKETVREREDKATVSERDKLLDRKILSICRGC"
     gene            16912..>17912
                     /locus_tag="VN968_10125"
     CDS             16912..>17912
                     /locus_tag="VN968_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL29630.1"
                     /translation="MAMAVTQGTLSASDHSAQLRKAVIASTIGTAIEWYDFFLYGTAA
                     GLVFGKLYFPNEDALTATLAAFGTYFIGFVGRPIGAAIFGHYGDRIGRKATLIATLLT
                     MGIATFLIAFVPTYASIGIWGAVILTVLRMLQGIGVGGEWGGSVLLAMEWSRTHGQRG
                     LVASWPQFGVPCGLFLANLAILAFSQLSGDQFLTWGWRIPFALSIILVGVGLWIRLGI
                     LETPVFQQLLDNRKIEKAPIVEVFRKHPKEIILSALLRMAEQAPFYIFTAFIFAYGVT
                     TLKMSRDFILIAVLAASCLSFVSIPLSGHISDLIGRRKMYLIGAATVGVFGFLYFAMV
                     "
BASE COUNT         3210 a         5605 c         5683 g         3414 t
ORIGIN      
        1 gaggacatcc agcgcgatct cgatcgccgc cggcccggac agtcgcgctt caccacccag
       61 cgccaggagc tagatgcggt aaaaattctc tccggcgtaa tggcgcatcc ggagaccggc
      121 gtgcaggtga cgaccggcac gccgatcgcg ctgttgatcg agaacaccga tcagcgctca
      181 aaagattatt ccgagatcaa ggacaagttt cgcccgggcc acgccgattt cacctatgag
      241 gcgaaatacg gcctgcgcga ttatcgcggc ggcggacgct cgtccgcgcg cgagaccgcg
      301 atgcgtgtcg cggccggcgc catcgcgcga aagatcgtgc ccgggatgac cgtgcgcggc
      361 gcgctggtgc agatgggccc gcaccagatc gatcgcgaaa aatgggattg ggacgagatc
      421 gcgcgcaatc cgtttttttg cccggacaag gacaaggccg cgttcttcga aagctatctc
      481 gacggtatca gaaaaaaggg atcctcgatc ggggcggtga tcgaggtggt cgcggaaggc
      541 gtgccggccg ggctgggcgc gccgatctat gccaagctcg attctgattt ggccgcggcg
      601 ctgatgagca tcaacgcggt caagggcgtc gagatcggcg ccggcttcgg tgctgccgaa
      661 ttgtcgggcg aggaaaatgc cgacgagatg cggatgggca atcagggcgt cagcttcctg
      721 tccaatcatg ccggcggcat tttgggcggc atctccaccg ggcagccggt ggtggcgcgc
      781 tttgcggtga agccgacatc ctcgatcctg tcgccgcgcc gcaccgtgga tcgtaacggc
      841 gccgacaccg acatcgtgac caagggccgc cacgaccctt gcgtcggcat ccgcgcggtt
      901 ccggtcggcg aagccatgat ggcctgcgtg ctcgccgacc atttcctgcg ccatcgcggg
      961 caggtcggca gcgcggcgcg caccgataac gctatctgat ttcgtatttg gctatttgta
     1021 tcgccttgac gtttcagaaa cttgcgccgc aaatgcatgc ctcatgaacg cagccgaact
     1081 gcagaaaatc accgactggc tcatcgatgg agcgaggtcc gcaccgaccc cgcctcagat
     1141 gatggcggaa gcctgcgaac gcctggtgag agcgggcttg ccgctgtggc gcgtcggcgt
     1201 gttcgttcgg acgctgcacc cggatatttt cggccgcaat ttcatctggc ggcccggagc
     1261 cgaggtcgag gtaggcacgg tcgattttgc tattctggat tcgccggagt ttcaaagtag
     1321 cccgctggca atcgtcttta gagagggaat agaggtcaga gcccgttcgg acgacccgga
     1381 gagcaaacgt ttcccgatcg ttgaagacat gcgcgcggaa ggcgtcaccg attatattgc
     1441 gctgccgctc ttgttcatgg atcgcgccgt tcacgcatcg agctggacga caaagcaggc
     1501 cggaggcttc agcgaggaac aactgaacgc tttgcggtcc gtgatcgcgc cgctggcgcg
     1561 gtacatcgag atcgtcagcc tgcgccgcac cgcctcgctc ctgctcgaca cctatgtcgg
     1621 caaccgcgcc ggcgagcgga ttctcggcgg gcagatccgc cgcggccaca ctgatacgat
     1681 ggacgccgcg atctggctgt cggatctgcg cggcttcacc gcgttgtcgg accggctgcc
     1741 ggccgaaacc gtggtcgata tcctcaacgg ctactttgac tgccaggtgt cagcgatcag
     1801 gacccacggc ggggaggtcc tgaagttcat gggcgacggg ctgctcgcgg tgtttccgat
     1861 tgacgagtat gtcggagaca cccagcaggt ttgctcgagc gtgctggaag ccgcgcgcga
     1921 atcccgagcc agcgtcgatg tgatgcatta cccgattgga gaaaccgtcg agcatttccg
     1981 cttcggagta gcgctgcatg ttggaaggat tcgctacggc aatatcggcg gcggcaaccg
     2041 cctcgacttc acctgtatcg gtcctgcggt caatcttgcg gcgcgactgg aaaaaatcgc
     2101 cggccggctt agtcgcacga tcgtggcatc tgcaggattt gcgggaatat gccccgacgg
     2161 ctgggccgat ttgggcgagt ttccgatcgc cggcttttcg aaggccgagc gcgtgtatgg
     2221 cttgcaggac gagacgcccg cggcgtgaaa ccggcaagat ctgctggcct cttgttattc
     2281 ctccggctcg gtggtgaaca gcagcgggta gcccttggcg cggccggcgt cggtggcgcg
     2341 cgtcgccttg gtctcggcga cgtccttggt gaagaccgcg accacgcata cgccgagccg
     2401 gtgcgcggtg atcatgacct tgtgggcctg gtcctcggtc atgcggaatt ccgccttcag
     2461 caccgtcacg acaaattcgc ggggcgtgaa gtcgtcgttg atgaggatga ccttgtgcag
     2521 gcgcggccgt tcggtcttgg tcttgacctt ggttttcggc ttggtgacgg cgtcgatcat
     2581 tccggctagc acctcctgac gtttttccga cgtttccgcg gtcgccggca aaagaataca
     2641 atctccgagc ccgctgtgga accggctgtt ttgcgatggc ttccttgcag cggcggcagc
     2701 gcgcgcagat agccctgcct gaaaaacctg ctggcgctcg tcgaccgagc gcaaggcgga
     2761 agccaatttg cgttgagcgc cgcagcagag gccttggtga aattaaggcg gtttgattga
     2821 cttgaatgtg taggacaagc ccatgtttct gcttttcgaa cgccggcttg catgtcacca
     2881 tcgcctcaag actacggagg accgctatgc gctggtgtgt ctctatcgga gcgttgcttg
     2941 tcgcgggagc gatggccgga ggcgacgttg ccggtatcgc cgcgccgaac cccacaaatg
     3001 cgttggcgga caaggacgcc gcatcctctg ttgacctcga actggtcctc gctgtcgacg
     3061 tctcctattc gatggacatg gacgaactgg cgatccagcg cgagggctac gcgcaggcca
     3121 ttatctcgaa ggaatttctg caggcgctga aaaccggccc caccggcaag attgcggtga
     3181 cctatttcga atgggcggca tccagtgacc agaagatcat cattccctgg cgggtgatcg
     3241 acgggccgga aaccgcggat gctgtggcca acgaaatcat gaagaccccg atccgccggg
     3301 cgtcgcggac gtcgatctcg ggcgcgatca atttcgcgat gccactgttc gacgagaacc
     3361 cctatcacgg actgcggcgt gtcatcgata tttccggcga cggtcccaac aacaacggcg
     3421 cgccggtgac cggcgcgcgc gacgcggcgc tggagaaagg catcaccatc aacggcctgc
     3481 cgatcatggt gaaggagccg tcctattcca cgatggacat cgacaatctc gatctctact
     3541 acgaggactg cgtgatcgga ggccccggct cgttcgtggt gacgatcaag gatcgcgaaa
     3601 agttcaagga ggcgatccgg accaagctcc tgctcgaagt cgccgaccgc acgcccgagc
     3661 gccggacggt cccggtcgcc gagaaggaga aggagccgcg cgtctcctgc ttgatcggcg
     3721 agaaaatctg gcaggatcgc tggggacggt aactcgcagc gacgtgcccc ggatgctgcg
     3781 caacgcgccg ctctttgcgg cgtggtgcgc tgctgatccg gggcccatga gagtttgggt
     3841 cccggctctg cggcgcatcg taaagagacg ctgcgccgcg tccgggacac gctacctcgt
     3901 aaacttcgcc atcaattcga catgcggcgt gtgccggaat tgatcgaccg gcgtcacccg
     3961 ctcgagtttg taaccgccgt cgaccagaat ccgcgcgtcc cgcgcaaagg tcgcgacgtt
     4021 gcacgacact ccaatcacga cagcaatctt gctggccgcg agctgaacgg cttgcgcctg
     4081 cgcgccctgc cgcggcgggt cgaacacgat cgcctcgtaa tcgcgcaact cctgcggcat
     4141 cagcgggcgg cggaacagat cgcgggcctc ggccttgacc ggcttcagtc cgggcgtcga
     4201 tgtcgccgcc ttctgcaacg ccgctaccgc gcccgcatcg ctgtcgaacg ccgaaacccg
     4261 cgattttgct gcaagccgca gcgcgaacgg tccgacccca cagaacaaat cggcgatgtg
     4321 cctggcgcgg gttaaatgtc cggataccag cgccgccagt gcttcttcgc cggcggcggt
     4381 ggcctgcagg aacgaacccg gcggcaaagt taactgcgct gcgccgatcg caatcgttgg
     4441 ggagttgcgc atcagcacca ggtcgccatg gcgcgtcagc cgcgccaggc ggtgttgctc
     4501 ggcaatgcgc gacagcgttg cgatcctggc cgccggcaac ggtcccgagc cgcgcacgtc
     4561 gacatcgagg ccgttgtcgg tcgcggtgat ctggatgtcg agcggctttc cgatcgatgt
     4621 caacggctcg gcgatagccc acccggccgc aagcgcgccg ttcaagccag gggccagaat
     4681 cggacagcga tcgatcggga taatgtcgtg cgaacctgct gccgcaaaac cgaccttgag
     4741 cacgtcgtgg gtgccgatgc gggcatgcag cgtaatccgc cggcgcccga ggccatgggc
     4801 atcgatcagc ggatcgactt cgcaggtgag cttggcctgc gccagcgtct cgatgacaat
     4861 atcgcgcttc catgcgcgat aacgctcact ttcccaatgc tgaatcgcgc agcccccgca
     4921 gacgccaaaa tgcggacaga atggcgtgac ccgctccggg ctggcgcgct cgaccgcgag
     4981 caggcggcgg cggtcgggat ggccgggtac ggcggaaacc tcgaccgtct cgccgccgag
     5041 cgtgtacgcc acgtataccg ccgcaccgtc cgcaaaggcg acgccgtcgc cgtgatgagc
     5101 gacgtgatcg atgacgagac gttcagccac gtcgagcgcc gatgaagaat tcggtattgc
     5161 cgtcgccacc cttgatcgat gacggaaaca cctggatatc gctacatccc agtgacgccg
     5221 cgaagcctgc gatgtcgttg cagatctgct ggtgcactgt tccatcgcgg acgatgccgc
     5281 gcctggaatg ctttcgcggc gcctcgaact gcggttttat caatgccagg aggtgcatcg
     5341 gcgcggccgc caatgacagc gccaccggca gcacggcttt cagcgaaatg aaactgacgt
     5401 cgatcacgac aacatccggc cgcgcgggca ggcgcttgcc ttcaaggctg cggatatcgg
     5461 tctgttccat ggaaacgacg ttgggatggc cgcgcagcga tggatgcagc tggtcgcgcc
     5521 cgacatcgat cgaaaacacc aggctggcgc cattggcgag caaaacctcg gtgaaaccgc
     5581 cggtcgatgc gccgacgtcg agacagacat gaccctcgac gtcgatcgga tattgctgta
     5641 gtgcgcccgc cagtttgacc ccgccgcgcg agacaaaggg atgcgcggct tgcgcctgca
     5701 acaccgcatc ggcggcgatg ctctcggacg gcttcatgac ctgcttgtca ttggccgtga
     5761 cgagtcccgc ttcgatcgcc gcctgtgccc gggcccggct ttcgaacagg ccgcgctcga
     5821 ccagcaatgt gtccgcgcgc ttgcgggcag gggacatgct ccgctcgccc tttgccatgt
     5881 tcaacttgaa gcacgcgcag gcgcagaggc cattcgcccg gccgccatgc gcgcgcaact
     5941 ctcaaacgca gtcaaatcgt gccagatgtc cgcgggtgct tgccggggcg tcatcagcgc
     6001 gcagccgtgc aggtcgagcg catggatcat catcaaattg tcggtcaacc cgaaattctc
     6061 gaccgtcaat cccgacgcat cgaccaggcg tccatcgcgt gaaatcaccc tgatgaattc
     6121 acctacgcga tcggcatagg acgagggatc gttggaatag cccaggcaaa gcttgccgcg
     6181 tcccgccata tagccgagtt cgtagacggt cccggcatcc gcgcccggtc cgcgaaatgg
     6241 cgtgagattg gcgataatgg catcggcctc gatcatcatg gcttcgttac cgcgaaatat
     6301 ctgcaaggag gcgtcgctgg ccgcgcggtc gatcgcattt tccagcggat acagtccgat
     6361 cagcccatgc tgcgcgcaca gcgcgacctt gcgccgtccg atatctgcgg catcgggcag
     6421 gaaaacgtcg gggccggcga gatagacttt tgtcattccg gatgcgccaa ccgaactcgg
     6481 gtttaccgga gttcggcaat atcattgtcc aagtcggcaa cagccgactt ggatgcgcag
     6541 gcccggaatc catactcccg atggtggtta tggattccgg gctcgctcgt ttcactcgcg
     6601 ccctcagcga caagcgctac gcgcttgcgc ggggaatgac acagggcgga acgtcaggcc
     6661 agctttaccg tctcggcctt gaaatccttg cccagcgtct cgaacacctt ggcaacgatg
     6721 cccttggcgt cgaggccggc gcgggcgtac atcgcgttcg gcgagtcatg gtcgaggaac
     6781 acgtcgggca gcaccatcga gcgcatccgc acgccgccgt cgagcatgcc gtgctccgcc
     6841 agcgtctgca tgacatggga gccgaacccg ccgatcgcac cttcctcgat cgtgagcagg
     6901 atttcgtgct cgcgcgcgag tttcaggacc agctcgacgt cgagtggctt catgaagcgc
     6961 gcgtcggcaa ttgtcgtgga cagtccatgg gccgccagtt cgtccgccgc cttctcgcat
     7021 tccccaagac gggttccgaa cgacagcagc gcgatcttgc tgccctcgcg aatgatgcgc
     7081 cctttgccga tctcaagcgg aatgccggct tccggcatct cgacgccgcg gccttcgccg
     7141 cgcggatagc gcagtgcgct cggacggtcg ttgatggcga cctgggtcgc gaccatgtgc
     7201 acgagctccg cctcgtccga cgccgccatg atgacgaagc cgggcaggca gccgagatag
     7261 gcgttgtcga acgagccggc atgggtcgca ccgtcggcgc cgaccaggcc ggcgcgatcg
     7321 atcgcgaagc gcacgggcag gccctggatc gctacgtcat ggacgacctg gtcatagccg
     7381 cgctgcagga aggtcgaata gatcgcgcag aatggcttgt agccttcggt tgcgaggccc
     7441 gccgcaaacg tcacggcatg ctgctcggcg atgccgacat cgaaggtgcg ctcgggaaac
     7501 gccttgcaga aaatatcgat gccggtgccc gacggcattg ccgcggtaat gccgacgatc
     7561 ttgtcgtctt tctgggcttc cttgacgagg gtctggccga acacgttctg ataggcggga
     7621 gcgttcggct tggccttggc ctgcgcaccg gtcgccacgt cgaacttgac gacggcgtga
     7681 tacttgtcgg cggaggcctc ggccggcgga tagcccttgc ccttttgggt gacgacatgg
     7741 accaggatcg ggccgtcctc catgtcgcgc acgttcttca agaccgggag cagatggtcg
     7801 aggttatggc cgtcgatcgg gccgacataa tagaaaccga gttcctcgaa cagcgtgccg
     7861 ccgccgacca tgaagccgcg cgaatattct tcgacacggt tggcgcggtc ggccagcacc
     7921 ttcggcagac gcttgttgat ctgttttgcg gcctcgcgca gcgaacggta ggtcctgccg
     7981 gaatacagcc gcgacaaata cgccgacatc gcgccgaccg gcggcgcaat cgacatgtcg
     8041 ttgtcgttga gaatgacgat cagccgggaa ttcatcgcgc cggcattgtt catggcttca
     8101 taggccatgc ccgcggacat cgcgccgtcg ccgatcacgg cgatgacatt gttcttgccg
     8161 ccggagaggt cgcgcgccac cgccatgccg agcgccgcgg agatcgaggt cgaggagtgc
     8221 gcggcgccga acggatcgta atcgctttcg gtgcgcttgg tgaaacccga caatccgccg
     8281 ccggtgcgca gcgtgcggat gcggtcgcgg cggccggtca ggatcttgtg cggataggcc
     8341 tggtggccga cgtcccagac caggcgatcg cgcggggtgt cgaaaatata gtgcagggct
     8401 gtcgtcagtt cgacgacgcc gagaccggcg ccgaaatgcc cgcccgttac cgagacggca
     8461 tcgatggttt cctgacggag ttcatcggcg acttgccgga cctgttccac cttgagccgg
     8521 cgcagatcgt cgggggtgtg gattgtgtcg agaagcggcg ttttgctaaa tacggtcacg
     8581 gcgatattcc aattttgcag gtcgggacga gcccccgacg tgagatgggt tgcgccgcat
     8641 cagcgcaacg aaaaacccag acgccggatg ccacctgagc aggccgaagc ctggttttaa
     8701 gtctagatgc gctccggatc gttcaatgtg atgtgcatca ccttcgacgg atttaccctt
     8761 cccagcgatt tttgtcagcc atttgaagtg cttactgctg cgagataatc cgcggcaggc
     8821 ccctttcaac agcccatagc cttaaagcct tacactaaag cgacgctcaa agccaacccg
     8881 ccatacccga ggattgggcg gccgcgagcc ggctttcaac cccggcggcg tgccgttggt
     8941 tccaactcgc ctaaatcggc ggcatcccca cggccgggaa ccgcggtttc gagcattttc
     9001 caggcgcatt cgcgctccgc ggcgtcgccg gtcgcaaacg ccagcgccgc ctcgggccga
     9061 gccatgcgtt cgcttaacgg gatcggcccg caaacgatca tgaaaaaatc gtaggcatgc
     9121 ggcagttcgt tggccatgac gaactggaaa tgcacccgaa agaacttcca gcggaacagc
     9181 ttgtaatgtc cgggctggat gatttcccgg aaccggaccg gcacgatcgt ggggttgcgt
     9241 cgggcggcgc cgacctcgat gctgtgtccg gcgatcggat cgaacggaaa gaagttcatc
     9301 acatccttgc gcgactggca gtcgatccag tcgatcgaag gttcaaccgc cagcaggcga
     9361 aggtgatcgc ggaaatcctg tgacgcggca tgaaatccga cgatcgggaa attgccgccg
     9421 atggtgagca agaccacccg cgggccatgc tggccgaggt ttgggtcgag cttcagcgcg
     9481 cgcgccataa tctcggcccc caaaaacgat cccgaactgt ggccgacgac gacgatttca
     9541 tcggcttcgc cctcgcgagc gacgtcgatg aggtatcggg caaaacggtc gatccgctca
     9601 tcccattccg gccgcttgcg gtgcgagaat tcccaggtcc agatcgtgtc cgcgagtaga
     9661 tagagcaggt aagtcgcgtt ctcgctgagc ttcagcacgg tgccgaggat tgcgaggaac
     9721 agggccgccg cggcggccat gctgaacggg tcggggatgc ccagcgcttc cagacctctc
     9781 gcgaaggcga aggcaatgcc gagcgcgcac atggcctcga tcagcagcag gaaatgcggg
     9841 taggtgatga aggtcgcgaa gcgccaattc gccttccaga atcgcgcgaa ggtaccgctg
     9901 aacacgacgc gccaatagat ccatatcgca tggaaaaccg tgcgccagat cggcgcggca
     9961 aggtcgcgct ggatcaggtc ttcccatcgc agaaaatcgt aacgggtacg ggtctgccag
    10021 tcgcttgcct cggtctcgat ggtccaggat gcggtctcgc cgtggggcgc aacctgcggg
    10081 cggctgacgg tggcgtcgag ctgatacagc cggccgaatt tccgcagctc ggttcggaac
    10141 atgcggtaat attgcgccag accgcgcgga tcgtatccct gcacatagat gatatggcgg
    10201 tgctgaacgc gcacttactg gtgatccccg agctggctcg gggactatat caggccatgc
    10261 ggcgtgccaa gagctatttc ggcccttgcg cgctcgtgtc gcgtcgattc accgatgttc
    10321 ccgcctgttg ccgggcgcgc gcctgcgcac cggggcgaca cggttgtcat tggacgtcga
    10381 gcggttcggt tccggtcggc tggccattgg cgtcggtggt gatcttgtcg acccgcgcct
    10441 ccgcttgccg cagcagttct tcgcatcgcc gtttcaacgc ctcgccgcgc tcgtagatgg
    10501 cgacggattc ttccagcggc accttgcctt cctcgagccg cttcacgatc gattcaagct
    10561 cctcgatcgc gcgctcgaag ctcagcttct tgacgtccat ttgggtattt tcggccatct
    10621 tgcttcccgg gttcgccctg tcgccggcgc ggacttaagc gtatttgtgc gggcgtattg
    10681 tggcggggat tcacacgccc atcaatgccg tgacatgcgc cgccaccgac tctttcaggc
    10741 cttgcagatc atagccgcct tcgagaaccg agacaattcg cccgccggcg cttgcatcgg
    10801 cgacatccat cagcttttgc gtcacccagc cgaagtcgtc cgcccttaga ttgagcgagg
    10861 ccagcggatc gcggtaatgt gcgtcgaagc cggccgagac gatgatcagt tcggggctga
    10921 atttccgcaa ctgcggcagg atcacgtttt cgaacgcggc gcggaattgg acgctgccgt
    10981 cttccgacgc cagcggcgcg ttgacgatgg tgtcgtgctc gccgcgctcg ccgcgcgcgc
    11041 cggtgccagg aaacagcggc atctggtgtg tcgagcaata catcaccgtc ggatcggacc
    11101 agaagatatc ctgggtgcca ttgccgtgat ggacgtcgaa atcgactacc gcggcgcggg
    11161 cgatgccgta tttgcgctgc gcgtggcgcg ccgcgatggc ggcatgatcg aacaggcaaa
    11221 agcccatcgg cctgttggtt tcggcgtgat ggcctggagg ccgcatcgcc acgaaggcgt
    11281 tatcatgcgt gcccgacatc acggcgtcgg tggcggcaac cgcgccgccg acgccgcgca
    11341 tcgccgcttc ccaggtgccc ggcgacatcg aggtgtcgcc atcgaggtag atcatgccgc
    11401 tcttgggcgc gatatgctgg agttgttcga tatagtgttc gttgtgacaa agcctgacca
    11461 gatcgagcga accttcggga gcctgttcgc gcaccagcct gtcaaagcgg ctttccccca
    11521 gaacctgctc gatcgcgcga atccggtcgg gacgttcggg atgcccgggc ggcgtttgat
    11581 ggtcgaggct tgagcgatgg ctaaccagca gcgtggtcat gcagtacctt ggcgcgcgaa
    11641 atgcgcgctt cttgagaagg ggctaatcta ggcggtcatt gaccgcccgg aaagagccga
    11701 agttgcggat atcgctttgc cattcagttc aaccgaacga tgcggtcggc tggctgaggc
    11761 gaaatcgggc tgttggcctg aaaacatccg tgcaaggcat cgacgtcgta gatgccgaat
    11821 tgccgcccgg ttccttcctt cagggcggta aaaccgtcgc cgccgaccgc gagataattg
    11881 ttcaccgtca cgcgatagcc cttcgcggga tcgatcggct gcccgttcaa cgacatgcga
    11941 tcggcgagca cgcggtcgcc gtacggcctt gcgttatccc atgtgtagtt gaaacccttt
    12001 gaaacctgca ggatccgcgg ccgtttcgga tcgagccatt gctgctcgag cgcgttcttg
    12061 atctgcgcgc cggtcagcgt cagtgtcacg agctggttcc ggaacggctg gctggaaaac
    12121 acatcggcgt agctcaccgt gccatccgcc tttttcggaa tgtcggtgcg gacgccgccc
    12181 ggattggtga aggcgatcac cgccccgccg tccttgtcgg cgcgggtcgc ggcgagttgc
    12241 gcgtcggcaa tgatatcgcc gagcgggctc tcgccggcat cgttgggcgc acgcgacagc
    12301 gtttccgtga tcgatcccgc cgggcggttg gcgatcggcg cggccacctt gtcgtaggac
    12361 gcgatcagcg cggtctgctc cggatcccgg gcataggcgg ccgtgcgcac gatcacattg
    12421 tcggccctgg cgctgatgat gtcgcgcgtt gcaggatcga gcctcacgtc gatcgcggtg
    12481 acgagggtgc cgtttttgtc gcccgaggtg acgagccggc cgtcgatctc gcagacatag
    12541 gcctgatggg tgtggccgct gatcaccacg tcgaccgcgt gatcgaattt cctgacgatg
    12601 tcgacgatcg gtccggatat gccgggacac tcatcataat cgccggtcgg aaatccgccc
    12661 tcgtggatca gcacgacgat ggcctcgacg ccgcgcgcct tcaattccgg caccagcgcg
    12721 ttgacggtgt cggcctcgtc cctgaattcg agaccggcga cgccgaccgg cgagacgagg
    12781 ttgggggtgc ccttcagcgt caggccgatg aaggcgaccg gtatgccctc gaattcccgg
    12841 atcgcgtaag gcgggaacac ggtcttgccc gtgtttttct cgatcgtgct tgccgcaaga
    12901 tagcggaatt tcgcgccgag gaacggatgc ggcccctggc atccgtcggt cggatggcag
    12961 ccgccattct gcatccgtag cagctcttcc ttgccctcat cgaattcgtg attgccgacg
    13021 gaagcaacct cgagccccat catcgacagc gattcgatcg tgggctcatc gtgaaacaac
    13081 gccgacagga acgggctggc gccgatcagg tcgccggcgg cgacaaaaat cgtgttgctg
    13141 gcgcccgcgc ggagctgctt gaccagcgtc gccatctgct cggcgccgcc ggcctccacc
    13201 gcgatctttt tactcctgtc gttgggatcg gcgatcctga tcccgcccgg cggcggctga
    13261 aggtagccgt ggaaatcgtt gatcgcgagg atcctcaact cgaccggcgc ggcttgcgct
    13321 tcggcgcgcg aacagggctc aagcgcaaac gccagcagca acgctgcgag caccgggcaa
    13381 agcggatgtc tcatgacttt acaccgaagc attttcgagt tcgctggctt catccttcga
    13441 gacgcatcgc ttcgcgatgc tcctcaggat gaggtctcag accctcatgg tgaggagcgc
    13501 ggcaaggccg cgcgtctcga accatgaggc ccgttggatg cctgatgata gtgcttcgcc
    13561 ccaaccggaa atgcccaaaa gctgcttctg ctcacgcctt ctgcctgcgc gaaagaaact
    13621 ccttgaaggc ggccactgcc tcgtgggagc gcatgcgttc gctgaacaga tggccctcct
    13681 ggtcaatccg gcgggtcagg tcttcgggcg gcggccgcaa cagcttgcgc gcggtcgcca
    13741 ccgcctccgc cggcagcgca caaatctcgc gcgccacctt gcgcgcctcg gcctcggtat
    13801 gaccgggcga caccacgacg ttgacgaagc cggcgatgcg ggcgtcctcg gcgctcatct
    13861 tgcgtcccat caccagcgtc gcgaacgcgc gctgatagcc catggtgcgc ggcatcaaca
    13921 ggctggaggc ggcttccgga accaggccca gctggatgaa cggcgtcgag aacgtcgcgg
    13981 tggtcgaggc gagcacaaag tcgcaatgga acagcatggt ggtgccgatt ccgatcgcga
    14041 cgccatcgac cgcggcgatg atcggcttca cgttgtgcgc aagcgaataa agaaattttg
    14101 tcatgttgga ggcgcgcggc gtctcggcgt tggcggtggc gtccttcagg aaatcgtcga
    14161 cgtcattgcc ggcgctgaac acgccggagc cgccggtgat gatcaggcag cggatcgcgg
    14221 gattgttctg cgcggtgtcg atcgcgtcgc tcatgccgcg atacatgtcc agcgtgatcg
    14281 cgttcttctt ttccggccgc cgcagcctga tcacccgcat cgcgccttcg tcggccacaa
    14341 tcaaatgtcc ggtcatgaag ccgtccttca gacaagcggc aaggtcgccg ctggcgagtc
    14401 tttgaaccgt atcctacatc atctccacgg cggcccaaac cggccgcgct tacttttcaa
    14461 cggcgacgca atgacgcgag ggccacgcaa tgcagttgac gggaattcac catctgaccg
    14521 cgatttccgc caagccgcgc gagaatctcg ccttttacgc cggcctgctc ggcatgcggc
    14581 tggtcaagaa gaccgtcaac caggatgacg tcagcgccta ccatctgttc tacgccgacg
    14641 ggcgcgccaa ccccggcacc gatctcacct tcttcgactt tccggccgcg ccggagcggc
    14701 gcggcagcaa cagcatttgc gctacctcat tgcgcgtcgc cggcgagcaa agcctcggct
    14761 tttggcggga tcggctgaag caggcgggcg gccacgccgg cgatatcagc gaagtcgacg
    14821 gccggctgac gctgccgttc gaggatccag aaggtcaacg cctggtgctg gtcgatgacg
    14881 gcggcgtcgg ttcggcctcg ccctgggaga agagcgcggt gccggccgag caccagattc
    14941 gcgggctcgg tccgatcatg cttagcgtgc gcgatctcgc ccgcaccgag caggtgctca
    15001 ctggtgtcat gaacatgcga agcgtgcgcg attacgccgc gccggatgcg cgcgcgcagg
    15061 ctcgggtgtt cgcgatgggc gagggcggcc cggcggcgga attgcacgtg atcgagcaga
    15121 aggatctgcc ggaagcgcgg cagggcgccg gcggcgtgca tcacgtcgcg ttccgcaccc
    15181 ccaacgacga gcaataccac gcctgggcgc gccggctgaa cgagcttggc gtccgcaata
    15241 gcggcgagat cgaccgcttc tattttcgca gtctttattt ccgcgagccg aacggcatcc
    15301 tgttcgagat cgccaccgac ggcccaggct tcgccaccga cgagccgatg gcagcgctcg
    15361 gcgagaaact ctcgctaccg ccattcctcg agccgcgccg cgcccagatc gaggccggcc
    15421 tgaagccgct tgaatagaac tgccggaaat caaaaatgag gtcgtcattc cgggatgtgc
    15481 ctctggtcgg caattgccga cctgaggcgc aggcccggaa tccatactcc cgatggtggt
    15541 tatggattcc gggctcgcgc aggagcgcgc cccggaatga cgactgtcat ttgtgatcgt
    15601 acgacggccc ggcctcgcgc gccgttgaaa tggcgtgagc aaatgttgcc tttaaatggg
    15661 tgatcgcttc accatctcga tcaagcgaag ccaaaccttg tggcttttca tatccgatgg
    15721 agcaattgaa tgacaaagct tcgccttctg ccggccgccg tgatcgccgc cgtcatgctg
    15781 acaacccctg tggtggccca ggagtatcat cccaacgcgc ggcaggccgt agcaagcgcc
    15841 cctgaccgcg ccgcgcctgg cgtgcgttac gttgacgggc gcctctgcta cccggctccg
    15901 cgcgtcggcg catttgccac ccagccctgg gagaacgaaa ccccttgcca gcccctgccg
    15961 gtctattgat ctgtccgcgc ggcgaaccgg cgagttcgct aaagcctgtt ccggctgagc
    16021 cgagtcatga ccgtctttca tggccgcatg gttcgagacg cgcggcgttg ccgcgctcct
    16081 caccatgagg gttcaagacc tcatcctgag gaacgcgctg ttgcgcgcgt ctcgaaggga
    16141 tgaagccact taacgggaaa atgcccgggc aaaccactcc ggcgcgagag ttcagcaccc
    16201 ccggcagatg ctcagaattt tgcggtcgag cagtttgtct cgctcgctga cggttgcctt
    16261 gtcctcgcgc tccctgaccg tttccttggc cccgcggcgg gatctggctg ccgcatatcg
    16321 cgtgcgtttg atcttcaccc cgcgctgcac cggcaaggtc cggaaggccg ttggcggcga
    16381 cacggtggac ggcatcgccg gacttatcga cggcggcggg atcgccggca ccctggccgc
    16441 gttcccgatg ccgcttggat cgttgaccga gccgtttaat ccgcgcgcat tcgccggccc
    16501 cggcggaatg ccgctgacgc cggcatcctg ggcgggcgat ctcgcaggcg ccgccagaag
    16561 accgagtact gtaacggcca gcaacgtcgc tcggccggcg atccatccgc gagacaacat
    16621 cgaaacaggc tgcgcgtggt tcatcagtga tcccttgccg ctggtccatt tctattccac
    16681 gggcagaact tgaaaagcgg agccaaagtt cccatggcgc gtagttggtt ggctcacctt
    16741 tcagcgtgca tgaacggact tcgtcacaaa ttcccgcgct gcagcaatcc gaatgcctta
    16801 gatgcgcctt ccggtcggcg cgaactgatt gtagcatcgt accgaatgac taagtgacct
    16861 gttgagaaac ggttttaggg gcagcgaaac aaccaaccaa gggacaattc aatggctatg
    16921 gcagtcacgc aagggacgtt gtccgcatcc gatcacagtg cgcagctgcg caaggcggtc
    16981 atcgcatcga ccatcggcac cgcgatcgag tggtacgatt ttttccttta tggcacggct
    17041 gccgggctgg tgttcggcaa gctctacttt cccaacgagg acgcgttgac ggcgacgctc
    17101 gcggcctttg gcacctattt catcggcttc gtcggacgcc cgatcggcgc cgctatattc
    17161 ggtcactacg gcgatcgcat cggccgcaag gctaccctca tcgccacgct tctgaccatg
    17221 ggtatcgcga ccttcctgat cgcattcgtg ccgacctacg cctcgatcgg catctggggc
    17281 gcggtcatcc tgacggtgct gcggatgctt cagggtatcg gcgtcggtgg cgagtggggc
    17341 ggttcggtgc tgctggcgat ggagtggtcg cgcacccatg gtcaacgcgg tttggtggcg
    17401 tcctggccgc aattcggagt gccgtgcggg ctgtttctgg caaaccttgc catccttgcg
    17461 ttcagccaat tgtcgggcga tcagttcctg acctggggct ggcgaattcc tttcgcgctc
    17521 tcgattattc tggtcggcgt cggactatgg attcgccttg gcatcctcga gacgccggtg
    17581 ttccagcagc tcttggataa caggaagatc gagaaggccc cgatcgtcga ggtgttcagg
    17641 aagcatccga aggagataat cctctcggca ttgctgcgga tggccgagca ggcgccgttc
    17701 tatatcttca ccgccttcat cttcgcttac ggcgtcacta ctttgaaaat gtcgcgcgat
    17761 ttcatcctga tcgcggtgct ggctgcctct tgcctctcgt tcgtcagcat cccgctgtcg
    17821 ggccacatct ccgacctcat cgggcgcagg aagatgtatc tgatcggcgc ggcaaccgtc
    17881 ggcgtgttcg gcttcctgta tttcgcaatg gt
//
LOCUS       DATNOI010000260         2795 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1376571,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000260 DATNOI010000000
VERSION     DATNOI010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376571"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VN968_10130"
     CDS             <1..489
                     /locus_tag="VN968_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HXL29631.1"
                     /translation="APKDKLPPLLQGFGAVPPLVTDIDLSMDDKFLYVACWGTGEMRQ
                     YDVADPRKPKLAGSVRIGGIVNRAPHPNGKAYAGGPQMVEISRDGKRVYWTNSLYSTW
                     DNQFYPDGVPGAEVMANASPSGGLALARDYWVSFPDGYRAHQIRLEGGDCSTDSFCYP
                     SV"
     gene            493..1161
                     /locus_tag="VN968_10135"
     CDS             493..1161
                     /locus_tag="VN968_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29632.1"
                     /translation="MNGTDWTPAGLWLAVVASGLFHGVNPGMGWPLAVSAGLMEKSSR
                     ALVAALWPLTAGHLLAMLLVLLPFALLVTLVEWQRQIQIGAAVLVIGFGIFRLINRRH
                     PRALARIRPTQLGLWSFAVAIAHGAGLMLVPIYLGLCRASDLDKGHQAAGALINFNLG
                     MAVLTSVVHSASMIAAGGLSAWLVYRYLGLKFMQRSWFNLETTWAVSLILVGAIALAF
                     SVTA"
     gene            complement(1213..1560)
                     /locus_tag="VN968_10140"
     CDS             complement(1213..1560)
                     /locus_tag="VN968_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrlR family regulatory protein"
                     /protein_id="HXL29633.1"
                     /translation="MKNGFYSIHIHMLDGVRGRDSGVFVLRNGVLIGGGPYFWSVGSY
                     TVDKGTWKGDLRTNQHTPFADRFVRPLFAGQEVTSGFSGTYADDRAEVFGTTLVGNRS
                     LSFRATLKRLADI"
     gene            1700..2149
                     /locus_tag="VN968_10145"
     CDS             1700..2149
                     /locus_tag="VN968_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL29634.1"
                     /translation="MDAVTPKGSQTADTHSHVVRPESMEWQKTRFPGCEAKTLLFDRT
                     TGLMTALMRFAPGAVLPDHEHVNIEQTYVLEGSLVDKEGPAKGIEAKAGEFIWREQGS
                     RHVAWCPEGGLMLAIFQVPNKFFEADGRVVDAAGQDWDETWGHTARQ"
     gene            2293..2793
                     /locus_tag="VN968_10150"
     CDS             2293..2793
                     /locus_tag="VN968_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXL29635.1"
                     /translation="MSRSSPTPEAVKSRPPLPPFTRETAAQKARMAEDAWNSRDPERV
                     ALAYTEDSRWRNRSEFFEGRAAIVAFLTRKWAREHEYRLIKDLWAFGDNRVAVRFQYE
                     WHDDAGQWHRSYGNEQWEFDEHGLMRRREASINDVAIAEADRRFRWAAPGPRPADVPG
                     LGESPF"
BASE COUNT          515 a          871 c          894 g          515 t
ORIGIN      
        1 gcgccgaagg acaagctgcc gccgctgctg cagggctttg gcgccgtgcc gccgctggta
       61 accgacatcg acctgtccat ggacgacaag tttctctacg tcgcctgctg gggcaccggc
      121 gagatgcgcc aatacgatgt cgccgatccg aggaagccga agcttgcagg ctcggtgcgc
      181 atcggcggca tagtaaaccg cgcgcctcac ccaaacggca aggcatatgc aggcggaccg
      241 cagatggtgg agatcagccg cgacggcaaa cgggtgtact ggaccaactc gctctactcg
      301 acatgggata accaattcta tcccgacgga gtgccgggcg ccgaggtcat ggccaatgcg
      361 agcccgagcg gcggtctcgc gctggccagg gactactggg taagcttccc cgacggatac
      421 cgtgcgcatc agatccggct cgagggcggt gactgctcga cggattcgtt ctgctatccg
      481 tcggtttgaa ttttgaacgg aacggattgg acacctgccg ggctttggct cgcggtcgtc
      541 gcgagcggcc tctttcacgg ggtgaacccg gggatgggat ggccgctcgc cgtttcagcc
      601 ggtctgatgg aaaagagctc gcgtgccctg gtggcggcgc tttggccgct gacggccggc
      661 catctgctgg caatgcttct agtacttctt ccctttgcgc tgctggttac actggtcgaa
      721 tggcagcgcc agatacagat cggcgccgcc gtgcttgtca tcgggttcgg catcttccgg
      781 ctcatcaacc ggcgccatcc acgggccttg gcgcggatac gaccgacgca actggggctc
      841 tggtccttcg ccgtggcgat cgcgcacggc gcgggactga tgctcgtgcc gatctatctc
      901 ggactctgcc gggcttccga ccttgacaaa ggccatcagg cggcaggagc cctcatcaac
      961 ttcaatctcg gcatggcggt cctgacctct gtggtccact cggcctcgat gatcgcggcc
     1021 ggcggattgt cggcgtggct ggtctatcgt tatctcggcc tcaagttcat gcagcggagt
     1081 tggttcaatc tggagacgac ctgggccgtc agcctcattc tggtaggagc tattgcactg
     1141 gcgttcagcg tcacggctta gaacggaagt cgcgtgtggc tgcaaaggcg gccatcgccg
     1201 gcgctcgacg catcaaatat ccgccaatct cttcaacgtc gcgcgaaaac tcaggcttcg
     1261 gtttcccacc agggtggtgc cgaatacctc ggcacggtcg tcggcataag tccccgaaaa
     1321 cccgctggtg acttcctggc cggcgaatag cgggcgaacg aagcggtcag caaacggcgt
     1381 gtgctggttg gtgcgcaggt cgcccttcca tgtccccttg tctacggtgt aggagccgac
     1441 cgaccagaaa taagggcccc cgccgatgag cacgccgttt cgcagcacga atacgccgct
     1501 gtcgcggccc cttacgccgt cgagcatatg gatgtgaata gagtagaacc cattcttcat
     1561 tgatacgccg ccgatcgtcc gggctgggaa cgataaagcc tatagcgatg gacgtcgagc
     1621 gaagccgtgg gagagccggc tgacgggcgc aagcaaagct gctatgatcg cttgaagttc
     1681 actgcaacgg agcattgcca tggatgccgt gacgccgaag ggttcccaga ccgccgacac
     1741 gcattcccac gtcgttcgcc ctgagagcat ggagtggcag aagacccgct tccccggctg
     1801 cgaagccaag acgctgctgt tcgatcgcac gaccggcctg atgaccgcgc tgatgcggtt
     1861 cgcgccgggc gcggtgctgc ccgaccacga gcatgtcaac atcgagcaga cctacgttct
     1921 tgagggcagc ctcgtcgaca aggaaggccc ggcgaagggc atcgaggcca aggccggcga
     1981 attcatctgg cgcgagcagg gcagccgtca cgtcgcatgg tgtcccgagg gcgggctgat
     2041 gctcgcaatc ttccaggtcc ccaacaagtt cttcgaggcc gacggccgcg ttgtcgatgc
     2101 cgcgggccag gattgggacg agacctgggg tcatacagcc aggcagtgag acctgttcgc
     2161 ttccgccttt ttccggcatc gaggggacgc aacccatttt cgacagcgct gccgacgctg
     2221 ccgagcgccg atcagatccg gctatcgggc gataggcaac tcgccatcga ctcgtcgcaa
     2281 ggcggcgata caatctcgcg gtcatcacca acgcccgagg ctgtcaagtc gcgtcccccg
     2341 cttccgccgt ttaccagaga gaccgccgcg caaaaggccc gcatggccga agacgcctgg
     2401 aattcgcgcg atcccgaacg cgtggcgctg gcctataccg aggacagccg ctggcgtaac
     2461 cgctccgaat ttttcgaagg ccgcgccgcg atcgtcgcgt tcctcacccg caaatgggcg
     2521 agggagcacg agtatcgtct gatcaaggat ctctgggcgt tcggcgacaa tcgcgtcgcg
     2581 gtgcggtttc aatacgaatg gcatgacgat gccgggcagt ggcatcgttc ctacggcaac
     2641 gagcaatggg aattcgacga gcacggcctg atgcgccgcc gcgaagccag catcaacgat
     2701 gtcgcgatcg cggaagccga ccgccgcttt cgctgggccg caccgggccc acggccggcg
     2761 gatgttccgg gattgggtga aagtccgttt tgaag
//
LOCUS       DATNOI010000261        10392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2478247,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000261 DATNOI010000000
VERSION     DATNOI010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10392
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2478247"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VN968_10155"
     CDS             <1..637
                     /locus_tag="VN968_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HXL29636.1"
                     /translation="VCGPSGSGKSTLIKCVNALEPFQGGEIILDGIKVNDPKTDLPKL
                     RARVGMVFQHFELFPHLKIIENLCLAQEKVLGRSHDEALAKGSKLLDRVGLKDHARKY
                     PAELSGGQQQRVAIARALAMDPIAMLFDEPTSALDPEMISEVLDVMVDLAREGMTMMV
                     VTHEMGFASKVAHRVIFMDQGEIVEDALKTDFFGSPRSDRAQKFLSKILSH"
     gene            833..1690
                     /locus_tag="VN968_10160"
     CDS             833..1690
                     /locus_tag="VN968_10160"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HXL29637.1"
                     /translation="MDQIAYVNGSFVPLSEAKVSVLDRGFLFADGIYEVAAVLDGKLI
                     DNASHLARLERSVGEIALALPETTARIQEIQRELVARNNLVNGMVYLEVTRGADAGRD
                     FAFPKGVKPTLVMFTSVKDIVNAPSAKTGIAVITVPDLRWERRDIKSVALLAQVLAKQ
                     AAAEAGAGEAWMIEDGKVTEGGSSSAFILTQDDVLVTRQNSSAILPGCTRKAVVALAQ
                     ERQLRVEERPFTVEEALKAKEAFITSATVFVQAVVSIDGKAVANGKPGPMTDRLREIY
                     VDFARATAQ"
     gene            1927..3117
                     /locus_tag="VN968_10165"
     CDS             1927..3117
                     /locus_tag="VN968_10165"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXL29638.1"
                     /translation="MVELGPPPGAVLSREKLSAIDDFVNGEIASGKIPGAIVLIQRHG
                     QQVYFKTFGKRDVEKGTPMTADAIFPIHSVTKTITSVAAMMLVDRGKIALDDPVSKYI
                     PSFAGTKVGVERKDDSGRAVLDLVPPRRPITIEDLLLHTSGITYGFYGEGLVKAAYDG
                     IYLGDFDNAEFVERVAKLPLAEQPRTLWDYGHSIDILGRVIEVVSGQSLFQFEKTNLL
                     DPLHMTTTKFFLTDPAERVRYAQPLAKDRHVERNSLDITRWESGGGGMVSTAADFARY
                     GQMLLNGGTLDGRTYLSPATFAAMTTDHIGPGSGVARNYFYYPGDGFGFGYGFGVRTD
                     PGNAVPPPPGSLGEIKWDGATGVYLVVDRAQDMFFVVMENSPSGRMHLQVTLKKIVYN
                     AFEK"
     gene            3161..4348
                     /locus_tag="VN968_10170"
     CDS             3161..4348
                     /locus_tag="VN968_10170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXL29639.1"
                     /translation="MLRKPDEKNQAIVTPLRIAAFDRARTFIILLVLVHHSVVNYTYF
                     GSGDKMRWIGFDLVVLFNDSFFMAFMFFISGLFVRDSLTRKGPAIFLRDRAWRLGVPY
                     LASVFVLMPIAYYPTFLRYHLPGTTDFNFLHFWWHTLTIGPWPSGPAWFLWVLLALDA
                     IVAAVWLVAPRAIGMLGQLTFSVRNRPTAAFVAFLIVSVAVYLPMHLIFGDASWLEPG
                     RFPLPIQTSRILLYTAYFFTGVGVGAAGLRAGLLAETGELARRWPVWLGFAVLFYGAI
                     LGLVYVHHNWVADFNSPPLLWRTGYGLAFAMFSAAMAFTVPAIFLRFAESGWRLLDAL
                     QPSAYGVYLVHYIFIIWLQYAVYDYSLPAGIKFAIVFIGTLAMGWGLTVMLRKIPIVA
                     RMI"
     gene            complement(4402..5475)
                     /locus_tag="VN968_10175"
     CDS             complement(4402..5475)
                     /locus_tag="VN968_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HXL29640.1"
                     /translation="MMRHWLIGLLLLVGPPAFAQQAVPSIQFDSVPNPLRLPPNIYFG
                     EVSGVSVNSKGHVFVLSRGNTSGPAYAAAATQLLEFDAKGGFVREIGKNLYAWSFGHT
                     VKIDPADNIWVTDKGSDMVIKFDPEGRVAMVFGRKQEASDEETGPVKHPKPPLPAEDG
                     RFRQVTDVAWDKAGNTFISDGYINSRVAKVDKDGNWLKSWGDRGKEPGQFNTPHSIAT
                     DAKGNVYVADRGNHRIQVFDGDGKFLRQFKIDVPVPPDAKPAIGKIPDEAMMAGGTFA
                     PGSPWSICITPPPNQVLYSSDAWPGRIYKLSLEGKVLGMLGQSGKQLKQFGWIHAMAC
                     PSENVIYAAELLNWRVQKLILKP"
     gene            5750..6652
                     /locus_tag="VN968_10180"
     CDS             5750..6652
                     /locus_tag="VN968_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL29641.1"
                     /translation="MAGYPDRLVKIVVPFAPGGGTDVIARTLAQEMAKDLGATVIIEN
                     KPGAGTIIGTQAVATSEPDGYTLLMGTFANAVNPSLNAKLPYDAHKDFVPVGLVARSF
                     NIVVVNPKSPFQSIADLIAAAKADPEKLSYGTFGTGTSAHLAGELFKNMAKVNLTTVP
                     YKGAAPAITDLIGGQIQVMFTTVASAASLIEGGQLRALAVTSAERSPAFPRLPTVAEA
                     GVPGYAAESWYGLFAPARTPADIIDRLNKSAGLAVQSEAFKKLGVNEGLVMVARPPQE
                     LDRYFRAEEERWRKVIQDAGIKVE"
     gene            6958..7575
                     /locus_tag="VN968_10185"
     CDS             6958..7575
                     /locus_tag="VN968_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29642.1"
                     /translation="MSGTTQRTLAALSLALICIASPASAADDTVRIRGTIERVEGPVY
                     VVKSRDGAELKLTVTDNPLFVAIAPSTMADIKPGMFVGSAGMMQPDGTQKAIEVHIFP
                     ESMRGTGEGHYDWDLKPQTKMTNGNVEQTVAGVDGPLLSVKYKDGEKKLVVTPETVVV
                     TYVPGNKDDLKPGTRIFVGAAKKQPDGTLQTPRITYGRDGAGPAF"
     gene            7657..8220
                     /locus_tag="VN968_10190"
     CDS             7657..8220
                     /locus_tag="VN968_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HXL29643.1"
                     /translation="MRSIVSLTLAACAIAVMSSPTLAQAQAQAPAAAPATPPAPPSAG
                     GTPDAMPFDIPYGQSIGLEHAKQVMAAAEAEAKKRNWKMNIAVVDTNGELVQFERMEG
                     AQIASGSISVGKARTAARFRRESRLFYNAFETGHGYVSTLDPTLVASPGGFPLVEGGK
                     LIGAVGCSGGTGDQDAAVCKAGAEVVK"
     gene            complement(8344..9330)
                     /locus_tag="VN968_10195"
     CDS             complement(8344..9330)
                     /locus_tag="VN968_10195"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL29644.1"
                     /translation="MRARRWFLSGGLCLGLIALTTAPIASAQEDPSKYPTRPVHIIVG
                     FSAGGGNDIIARIFGQKLSEGLGQPVIVENKPGGGAIVATEYVAKSAPDGYTLLVGAS
                     GMAINPAVYARLPYDSIRDFVAVSELASFPLILIVNPSSPIKSVADLVAYAKANPDKA
                     NYASSSASFQLVTELFKQKTGAPMQVIPYKGANDSVMAVISGQVTAAIADAGPALSQV
                     LGGQARALAVAAPKRMEELPDVPTLKEAGADVDAVLWSGLFAPKETPPAIVKKLEGEI
                     MRIARLPEVSSRLKPLGILSVGNSSEEFGRILAADIARWAAVAKAGNIKIEQ"
     gene            complement(9342..>10392)
                     /locus_tag="VN968_10200"
     CDS             complement(9342..>10392)
                     /locus_tag="VN968_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXL29645.1"
                     /translation="AYLGRKPDAVAVLERLARTCGIRVAEFNSIDLNISQDSPCFAGF
                     DPLPLLETADLGLLLDSDVPFIPQYARHAGAIKWIQIDVDPLKSDFPMWGFPTDMRIQ
                     GDCAIVLRQLLDAVEARADDAWRKRVAERIASWSGARDATVKRRSAAGANKGISGALN
                     PAFLFATLNAKLSQDDVVINEAIRNAPILQEHITRTRPSSYVGLAGGGLGFSGGMALG
                     LKLAQPERRIVQVIGDGGFHFSSPDSVYAVAQQYQIPVLTVVLDNGGWQAVKSAVQRV
                     YPKGIAAETDQFQSRLRSGRQGEQRDFSAVARAFGAHGECITEPDDLDSAIDRCLSAL
                     GDGKAAVLHVHITPL"
BASE COUNT         1924 a         3194 c         3254 g         2020 t
ORIGIN      
        1 ggtatgcggt ccgtcgggat cgggaaaatc gacgctgatc aaatgcgtca acgcgctgga
       61 gccgttccag ggcggcgaaa tcatcctcga cggcatcaag gtcaacgatc ccaaaaccga
      121 cctgccgaaa ctgcgcgccc gtgtcggcat ggtgttccag cattttgagc tgttcccgca
      181 cctgaagatc atcgagaacc tttgcctggc gcaggaaaag gtgctgggac ggtcgcatga
      241 cgaggctctc gcgaagggct caaaactgct cgaccgcgtc ggtctgaagg accacgcgcg
      301 caaatatccg gccgaactgt ccggcggcca gcaacagcgc gtcgccatcg cgcgggcgct
      361 ggcgatggat ccgatcgcca tgctgttcga cgagccgacc tcggcgctcg atcccgaaat
      421 gatctccgag gtgctcgacg tcatggtcga cctcgcccgc gagggcatga ccatgatggt
      481 cgtcacccat gaaatgggct ttgccagcaa ggtcgcgcac cgggtgattt tcatggacca
      541 gggcgaaatc gtcgaggacg cgctgaagac cgatttcttc ggcagccccc gcagcgaccg
      601 cgcgcaaaag ttcctgtcga aaatcttgtc gcattaggac ccgatgtcat tccggggcgt
      661 cgaagaggcg aacccggaat ctcgagattc cgggtctggt cctgcggacc atcccggaat
      721 gacatcggat ctttgcacgg gctatcgcga attccgtttc caattcgtcg atcaagccct
      781 aagaatttgt gtatacatgc ggcgcaactg tttgcccgat aggagaaatc gcttggatca
      841 gatcgcttac gtcaacggtt cgttcgtgcc tctcagtgag gccaaggtct cggtactgga
      901 ccgcggtttt ttgttcgccg acggcatcta cgaagtcgct gccgtgctcg acggcaagct
      961 gatcgacaat gcctcgcatc tggcgcggct ggagcgctcg gtcggcgaga tcgcgctggc
     1021 cttgccggaa acgaccgcgc gcattcagga gatccagaga gaactggtcg cgcgcaacaa
     1081 cctggtcaac ggcatggtct atctcgaagt gacgcgcggc gccgacgccg gacgcgactt
     1141 tgcgtttcca aaaggcgtca agccgacgct ggtgatgttc acgtcggtca aggacatcgt
     1201 caacgcgccg tcggccaaga cgggtatcgc tgtcatcacc gtgcctgatc tccgctggga
     1261 gcggcgcgac atcaagagcg tggcgctgct ggcgcaggtg ctggcaaaac aggccgcggc
     1321 ggaggccggc gccggcgagg cctggatgat cgaggatggc aaggtgaccg agggcggctc
     1381 gtcctcggcc ttcatcctga cccaggatga cgtgctggtg acgcggcaga attcatccgc
     1441 gatcctgccg ggctgcaccc gcaaggcggt ggtcgcgctc gcccaggagc gccagcttcg
     1501 cgtcgaggaa cggccattca cggtcgagga ggcgctcaag gccaaggaag ccttcatcac
     1561 cagcgccacc gtgttcgtgc aggcggtggt gtcgatcgac ggcaaggccg tcgccaacgg
     1621 caagcccggc ccgatgaccg accgcttgcg cgagatctat gtggactttg ctcgggccac
     1681 ggcgcagtag cgcctgtctc gctcgccgcc aaaggcggta ttcccgttta ttcccgatca
     1741 cgatttccac aagcaggtac tcaccgtctg gatcgggctc cggcaatacc gtaacttcgc
     1801 ttcgtccaac ggatcggtca ggcaaaagag atgtctccgc acgacattgc gtcaagccgc
     1861 cgctgcgcgg tgcggttcgc cgcgatggcc gcggtcgtct gcgtcgcagc ccccttatcg
     1921 gccgcgatgg tcgaacttgg gccgccgccg ggggccgtgc tgtcgcgcga aaaactttca
     1981 gccattgacg atttcgtcaa tggcgaaatc gcctccggca agatccccgg cgccatcgtt
     2041 ctgatccagc gtcacggcca gcaggtctat ttcaagacct tcggcaagcg cgacgtcgag
     2101 aagggaacgc cgatgacagc ggacgccatc ttcccgatcc attcggtcac caagaccatc
     2161 accagcgtgg cggcgatgat gctggtcgac cgcggcaaaa tcgcgctcga tgatcccgtc
     2221 agcaaataca tcccgtcctt cgccggcacg aaagtcggcg tcgagagaaa ggatgattcc
     2281 ggccgcgcgg tgcttgacct ggtgccgccg cgccggccca tcactatcga ggacttgttg
     2341 ctgcacacgt cgggcatcac ctacggtttc tatggtgagg ggctggtcaa ggccgcctat
     2401 gacggcattt atctcggtga tttcgacaat gccgaattcg tagagcgggt ggcaaagctg
     2461 ccgctcgccg agcagcctcg cacgctctgg gattacgggc attcgatcga catcctcggc
     2521 cgtgtgatcg aggtcgtgtc cggccagtcg ctctttcagt tcgaaaagac aaacctgctc
     2581 gatccgcttc atatgaccac cacgaaattc ttcctgaccg atcctgcgga acgggtgcgc
     2641 tacgcccagc cgctggccaa agaccgccat gtcgagcgaa actcgctcga catcacgcga
     2701 tgggaatccg gtggcggcgg aatggtctcc acagcagccg atttcgcgcg ttatgggcag
     2761 atgctgctga acggcgggac gctcgacggc agaacctatc tcagtcccgc gaccttcgcc
     2821 gcgatgacca cggatcacat cgggccgggg tcgggcgtcg cgcgcaacta tttctattat
     2881 cccggcgacg ggttcggatt cggctatggt tttggcgtcc gcaccgatcc cggcaatgcg
     2941 gtgccgccgc cgccgggctc gctcggggag atcaaatggg atggcgccac cggcgtctat
     3001 ctcgtggtcg accgcgccca ggacatgttc ttcgtcgtaa tggaaaattc gccatcgggg
     3061 cgcatgcacc ttcaagtgac cctgaaaaag atcgtataca acgcattcga gaaatgatct
     3121 gaaactaccg gctgatgggt cttggcatgg aacggaattg atgctgcgga aaccggacga
     3181 aaaaaatcag gccatcgtga cgccgctacg catcgcagcg ttcgatcgtg cgcggacgtt
     3241 catcatcctc ctggtgctgg tgcaccattc tgtcgtcaat tacacctatt tcggcagcgg
     3301 cgacaaaatg cgctggatcg gttttgatct ggtggtgctg ttcaacgaca gtttcttcat
     3361 ggcattcatg tttttcatct ccgggctgtt cgtccgcgac agcctgacgc gaaagggccc
     3421 tgcgattttc ctgcgcgacc gcgcgtggcg gctcggcgtg ccgtatctgg cgtcggtgtt
     3481 cgtgctgatg ccgatcgcgt attatccgac attcctgcgc taccatctgc cgggaaccac
     3541 ggatttcaac ttcctgcatt tctggtggca cacgctcacc atcgggccgt ggccttcggg
     3601 gccggcgtgg ttcctgtggg tgctgctggc gcttgacgcg atcgtcgccg ccgtctggtt
     3661 ggtcgcgcct cgcgccatcg ggatgctcgg ccagctgact ttttccgtac gcaatcggcc
     3721 gacggcggct tttgtcgctt ttctgatcgt ctcagtcgcg gtctatctgc cgatgcactt
     3781 gattttcggc gacgctagct ggctcgaacc tgggcgattt ccgctgccga tccagaccag
     3841 ccggatcctg ctctacaccg cctacttctt taccggcgtc ggcgtcggcg ccgcaggcct
     3901 tcgggccggg ctgctcgcgg aaaccggcga actcgccaga cgctggccgg tctggctcgg
     3961 ctttgcagtg ttgttctacg gcgcgatcct cggcctcgtt tacgtccacc acaactgggt
     4021 agcggatttc aactcgccgc cgctcttgtg gcgaacaggt tacggcctgg ccttcgcgat
     4081 gttcagcgcc gcgatggcgt tcacggtgcc ggcgattttc ctgcggtttg ccgaatccgg
     4141 ttggcgcctg ctcgatgcgc tgcagccttc ggcttacggc gtgtacctcg tgcactacat
     4201 tttcatcatc tggctgcaat acgccgtcta cgattactcg cttcccgcag gcatcaagtt
     4261 cgcgatcgtg tttatcggca cacttgcgat gggctggggg cttacggtga tgttgcgaaa
     4321 gattcctatc gtggcgcgga tgatttgaga gccgtcattc cggggcgatg cgaagcatcg
     4381 aacccggaat gacggccggc ctcacggctt caggatcagc ttctgcaccc gccagttcag
     4441 caactcggcg gcgtagatca cgttctccga gggacacgcc atcgcgtgga tccagccaaa
     4501 ctgcttgagt tgcttgccgg attggccgag catgccgagc accttgccct cgaggctgag
     4561 cttgtagata cggccgggcc acgcgtccga actgtacagc acctgattgg gtggcggcgt
     4621 gatgcagatc gaccacggcg aacccggcgc aaaggtgccg cccgccatca tcgcctcgtc
     4681 agggatcttg ccgatcgcag gttttgcgtc cggcggcacc ggcacgtcga tcttgaactg
     4741 gcgcaagaat tttccgtcgc cgtcgaacac ctgaattcga tggttgccgc gatcggcgac
     4801 atagacattg cccttggcgt cggtcgcgat ggagtgcggg gtgttgaact ggccgggctc
     4861 cttgccgcgg tcgccccatg acttcagcca gttgccgtcc ttgtcgacct tggcgacgcg
     4921 ggaattgatg tagccgtcgc tgatgaaggt gttgccggcc ttgtcccacg cgacatcggt
     4981 gacctgacga aaccggccat cctcggcggg cagcggcggc ttcggatgct tgaccggtcc
     5041 ggtctcttcg tcggaggctt cctgcttgcg gccgaacacc atggcgacgc ggccctcggg
     5101 atcgaacttg atcaccatgt cggagccctt gtcggtcacc cagatgttgt cggcaggatc
     5161 gatctttacc gtgtgcccga acgaccaggc atagaggttc ttgccgattt cgcggacgaa
     5221 cccgcccttc gcatcgaatt cgagcaactg cgtcgccgcc gcggcgtagg ccggcccgga
     5281 ggtgttgccg cgcgacagca cgaaaacgtg gcccttggaa ttgaccgaga cgcccgacac
     5341 ttcgccgaaa tagatgttgg gcggcagccg gagcggattc gggaccgagt cgaattggat
     5401 cgagggcacc gcctgctgtg cgaaggccgg cggcccgacc aacagcaata gcccgatcag
     5461 ccagtgcctc atcatcatat cctcccggag cattcgttgt ttttttgacg tcgtagcgga
     5521 tgctagtcta tcgctattcc ctgcgtccat cgccagcagg cgcgttcccc gtcatatccg
     5581 ttcgccgccc ggcgcatggc gctgcgcggc aaggcatgat ctgctggtgc gcttttcgaa
     5641 ctccagtcgc atgcggtccc ctatgctttt tgaacgcaac ataacgacga cgtctcttct
     5701 gttcgcgctt gcagcattat tgcccgctgc gttgttggcg aatggtgctt tggccggtta
     5761 ccccgaccgg ctcgtcaaaa tcgtcgtgcc gtttgcgccg ggcggcggca ctgacgtgat
     5821 tgcgcggacg ctggcgcagg agatggcaaa ggacctcggc gctaccgtga tcatcgagaa
     5881 caaaccgggg gcggggacga tcatcggtac ccaggccgtc gcaaccagcg agccggatgg
     5941 ctacacgctg ctgatgggaa cattcgccaa tgcggtcaat ccgagcctca atgcgaagct
     6001 gccctacgat gcgcacaagg actttgtccc ggtcgggctg gttgcgcgct ccttcaatat
     6061 tgtcgtggtc aatcccaaat cgccttttca atcgatcgcg gatctcatcg cagcggcgaa
     6121 agccgatccg gaaaaattgt cctacgggac atttgggacg ggcacgtcgg cgcatctcgc
     6181 gggcgagctg ttcaagaaca tggcgaaggt caatctgacc acggtgcctt acaagggcgc
     6241 cgcgcctgct atcaccgatc tgatcggcgg ccagatccag gtgatgttta cgacggtcgc
     6301 aagtgctgcc tcgctgatcg agggcggcca gttacgggcg ctcgccgtga cgtcggcgga
     6361 acgctcgccg gcctttccgc gactgccgac cgtcgccgag gccggggttc ccggctatgc
     6421 cgcggagagc tggtatggcc tgttcgcgcc ggccaggacg cctgccgaca tcatcgaccg
     6481 cctcaacaaa tccgcaggcc tggcggttca atcggaggct ttcaagaagc tcggcgtgaa
     6541 tgagggtctc gtcatggtcg cgcggccccc gcaagagctc gatcgatatt ttcgcgccga
     6601 ggaggaacgc tggcgcaagg tgatccagga cgccgggatc aaggtcgaat agcggcggct
     6661 ccggttgcgg aaggccgcgg tcgaaagttt gttgccggct tgcaggaata ttcgcggagc
     6721 gcgggtgttg cagaggggcg tccgtagtgc gcaacccgtt gcgctactct ttgaagtatc
     6781 aagaaaaaag actggaaccg ggggaaacgc gatgaggaca ccggcgaaca cctcgcccga
     6841 ggccaaggag gcctttgcgg aaatcgccgg cttcttcgac aagcatctgg gaaatcggcc
     6901 gccgatggaa taacgaaaga gtcagccgtc aattcgaaaa tccagggagg attaacaatg
     6961 tccggcacca cacagcgtac ccttgctgcc ctaagcctcg ctctgatctg catcgcgtcg
     7021 ccggcatcgg cggcggatga taccgtccgc atccgcggca ccatcgaacg cgtcgaaggt
     7081 ccggtttacg tggtcaagag ccgtgatggt gccgaactga agctgaccgt caccgacaat
     7141 ccgctgtttg tcgcaatcgc tccctcgacc atggcggaca tcaagccggg catgttcgtc
     7201 ggctcggccg gaatgatgca gcccgacggc acccagaagg cgatcgaggt gcatatcttc
     7261 ccggaatcga tgcgcggcac cggcgaaggc cattacgact gggatctcaa gccgcagacc
     7321 aagatgacca atggcaatgt cgagcagacg gttgccgggg tcgatggacc gctgctgtcc
     7381 gtcaagtaca aggatggcga gaagaaactg gtggtgaccc cggaaaccgt tgtggtgacc
     7441 tatgtccctg gcaacaagga cgatctcaag ccgggcacca ggatcttcgt cggcgccgcg
     7501 aagaagcagc ctgacggcac gttgcaaacc ccgcgtatta cctacggcag agacggcgcg
     7561 ggaccggcat tttgactccg atagggcgtc gcgcatagcg tcgcggcact gtcgacagcc
     7621 tttacaacgg aagcagactt caacctggag gatactatgc gttcaatcgt ttcgctgaca
     7681 ctggcagcct gcgccattgc ggtcatgagt tcgccgacac ttgcgcaagc acaagcacaa
     7741 gcgccggcgg cagcgccagc aacgccgccg gcgccgccgt ccgcgggcgg tacgcccgat
     7801 gcgatgccgt tcgatattcc ctacggtcag tcgatcgggc tcgaacatgc caagcaggtg
     7861 atggcggcgg ctgaagcgga ggccaaaaag cgaaactgga agatgaacat cgccgtggtc
     7921 gataccaacg gcgaactcgt ccagtttgag cggatggagg gcgcgcagat cgcatccggc
     7981 tcgatttcgg tcggcaaggc gcgcaccgcg gcacgctttc gccgcgaatc ccgcctgttc
     8041 tacaacgcgt ttgaaaccgg ccacggctat gtcagcacac tcgatccgac cttggtcgcc
     8101 agtcccggcg gatttccgct ggtcgaaggt ggcaagctga tcggcgcggt tggctgcagc
     8161 ggcggcaccg gcgatcagga cgccgcggta tgcaaggccg gcgctgaggt cgtgaaatag
     8221 cacggcgttt tctgctccgg cgcgacgaag cgccgggttt cgttggcgcc gtggcgcgac
     8281 accgcacgcg gcgccaaaac gagcgttatt gtcgcgcgta tctttgcccg accggcgagg
     8341 gcgctattgc tcgatcttga tattgccggc cttggcgact gcggcccagc gcgcgatatc
     8401 ggcagccaga atccggccaa actcttcgga cgaattgccg accgacagga tgccgagcgg
     8461 tttcaagcga gagctcacct ccggcaatcg cgcgatgcgc atgatttcgc cctcaagctt
     8521 tttgacgatc gcgggcggcg tctccttggg cgcgaagagg ccgctccaca gcaccgcatc
     8581 gacatcggcg ccggcctctt tcaaggtcgg cacgtcaggc agttcctcca tccgtttggg
     8641 cgccgcaacc gcgagcgcgc gggcctggcc tcccagcacc tggctcaacg ccggaccggc
     8701 atcggcgata gccgcggtga cctgacctga gatcaccgcc atgaccgaat cgttggcgcc
     8761 cttgtaggga atgacctgca tcggcgcacc tgtcttttgc ttgaacaact cggtcaccag
     8821 ctgaaacgag gcggacgagc tcgcataatt tgccttgtcc ggattggcct tggcgtaggc
     8881 gacaaggtcg gcgaccgatt tgattggcga cgatgggtta acgatcagga tcagcggaaa
     8941 cgatgcgagt tcggagacgg caacaaaatc ccggatcgag tcgtatggca gcctggcgta
     9001 gacggcaggg ttgatggcca tgccgctggc gccgaccaat agcgtgtagc cgtcgggcgc
     9061 cgacttcgcg acatattcgg tggcgacgat cgcgccgccg cctggcttgt tttcgacaat
     9121 gacgggctgg ccgaggcctt cggacaattt ttgcccgaaa atccgcgcga tgatgtcgtt
     9181 gccgccgccc gcgctgaagc cgacgatgat gtgaacaggc cgggtcggat atttcgatgg
     9241 gtcttcctgc gcggaagcga tcggggctgt ggtgagcgcg atcaatccaa gacaaagacc
     9301 gcctgacaga aaccagcgac gggccctcat cggcttgttc ctcagagcgg cgtgatgtga
     9361 acgtgcagca cggcggcctt gccgtcgccg agcgcagaca ggcagcggtc gatggcggaa
     9421 tcaagatcgt cgggttcggt aatgcattcg ccatgggcgc cgaacgcccg cgcgacggcc
     9481 gaaaaatccc gctgctcgcc ctggcggccc gatctcagcc gcgactgaaa ctgatcggtt
     9541 tcggcggcaa tgcccttggg atacacccgc tgcacggcgg atttcaccgc ctgccagccg
     9601 ccattatcca gcacgacggt tagaaccgga atctgatatt gctgcgcgac cgcatagacg
     9661 ctgtcgggcg atgagaaatg aaatccgccg tcgccgatta cctgcacgat gcggcgctcc
     9721 ggctgcgcca gttttaatcc aagcgccatg ccgcccgaga atccaagccc gccgccggcc
     9781 aggccgacat agctcgaagg cctggtgcgc gtgatatgct cctgcaggat cggcgcgttg
     9841 cggatcgctt cgttgatgac aacgtcgtcc tgcgacagtt ttgcgttcag cgtggcgaac
     9901 aggaaggccg ggttgagcgc gcccgaaatg cctttgttgg cacccgcggc ggaacggcgt
     9961 tttacggttg cgtcgcgtgc accgctccag cttgcgatgc gttccgcgac gcgtttgcgc
    10021 caggcgtcgt cggcgcgcgc ctccacggcg tcgagcaact gccgcagcac gattgcgcaa
    10081 tcgccctgga ttcgcatgtc ggtcggaaat ccccacatcg gaaaatccga cttcaaggga
    10141 tcgacatcga tctggatcca tttgatcgcg ccggcatgcc gggcgtattg cgggatgaac
    10201 ggtacatccg aatccaggag caggccgaga tcggcggtct cgagcagggg taggggatcg
    10261 aagccggcga aacagggcga atcttgcgag atattgaggt cgatcgaatt gaattccgcg
    10321 acccggatgc cgcaggtccg cgccagcctc tccagcaccg cgacggcgtc cggcttgcgg
    10381 ccgagatagg cg
//
LOCUS       DATNOI010000262         8889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_816663,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000262 DATNOI010000000
VERSION     DATNOI010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816663"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VN968_10205"
     CDS             complement(<1..289)
                     /locus_tag="VN968_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL29646.1"
                     /translation="MTAPLDPDIEIIIPLLPLRDPETMTPQSAREALRALAAARAAIP
                     PPPVASADDIKVKGAAGPLAARVYRVSREKSPTVVFFHGGGWVAGDLETHDR"
     gene            complement(390..1586)
                     /locus_tag="VN968_10210"
     CDS             complement(390..1586)
                     /locus_tag="VN968_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL29647.1"
                     /translation="MAHDEPQGAGPTRLVIRNIGLLLSGAMEKPILDADTILAENGRI
                     TAIGRAKDIDAEGATTLVDANGTTVAPGLIDSHVHPVAGDWTPRQNQINWIDSFLHGG
                     VTTMISAGEVHMPGRPRDVVGLKAMAIFAQRSFWTLRPGGVKVHAGAPVIENEMVEED
                     FRELAAAGVKLLGEVGLGGVKDGPTARKMVGWARKYGIQSTIHTGGPSIPGSGLIDKD
                     VVLEADTDVIGHINGGHTALPDDQIRCICEGCKRGLELVHNGNERSALYTLRIAREMG
                     DLSRVILGTDSPAGSGVQPLGILRMVSLLSSLGEIPAEIAFCFATGNTARMRALDCGI
                     IEVGRSADFVIMDKAQHSPGKNILDSVQLGDLPGIGMTIIDGIVRTQRSRNTPPATRV
                     PEIVGH"
     gene            1762..2535
                     /locus_tag="VN968_10215"
     CDS             1762..2535
                     /locus_tag="VN968_10215"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HXL29648.1"
                     /translation="MSSFNQESVLSVHHWTDNLFSFTTTRDPSFRFRNGEFTMIGLKV
                     GEKPLLRAYSVASANYEDRLEFFSIKVPDGPLTSRLQHLKQGDEIIVSRKATGTLVID
                     NLEDGRNLYLIGTGTGLAPFLSVIKDPETYERFEKVVLLHGCRRVAELAYGEMITENL
                     PKDELIGELVRNQLIYYPTVTRDPFRNRGRITDLITSGKLFSDAGLAPLESAHDRVMI
                     CGSPALVHDTRALLSGKDFVEGNHGEPGQFLVEKAFAER"
     gene            complement(2532..2882)
                     /locus_tag="VN968_10220"
                     /pseudo
     CDS             complement(2532..2882)
                     /locus_tag="VN968_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407622.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3155..3280
                     /locus_tag="VN968_10225"
     CDS             3155..3280
                     /locus_tag="VN968_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29649.1"
                     /translation="MNNKTQPADRGAAIYKALWHAIIERALQPGAKLPEDAIGEK"
     gene            3290..4612
                     /locus_tag="VN968_10230"
     CDS             3290..4612
                     /locus_tag="VN968_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL29650.1"
                     /translation="MSFDTILRNGCIAGREHEPVDIAVRDGRIAAITPRSNQATQDAA
                     CDIALDGKLVIPGFVETHIHLDKSCISDRCSCRTGTLQEAIESVAAAKLAFTEQDIYA
                     RGRRTLEKAIVQGTTGMRAHVEVDPRIGLKGFRAVSQLKRDFAWAVDIEICVFPQEGL
                     LNDPGCDELLVQACDDGADLIGGCPYTDNDPHGQIARIFELAKRYDLDIDLHLDFDLD
                     PSWMHLDEVCRQTAKYGWGGRVAIGHVTKLSALDHAALEKIGVKLANAGIAVTVLPAT
                     DLFLMGRGAERSVPRGVAPAHRLLAQGVTCSLATNNVLNPFTPFGDCSLLRMANLYAN
                     IAQLGRPDELQACMDMVTLLPAKLMNLGDYGIAVGNPADIVVLDCADRVSAVTELAQP
                     LLAMKRGRISFSRPTATLYWPGATPSLVKDDFGAGPFADARSLARSLP"
     gene            4688..5518
                     /locus_tag="VN968_10235"
     CDS             4688..5518
                     /locus_tag="VN968_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HXL29651.1"
                     /translation="MADLSKGVPADLRVAIAGLGPIGKRVAEALDRGIDGLALVAVSA
                     QHPEKHRSWLSGLTRTPAVLPIDALCDVADIVIECAPGKLVRSIVEPFATSGKTAVVL
                     SAGALLDHEDLIEIAKRNGGQIVVPTGALIGLDAVTAAAVGTIHSVRMVTRKPIAGLV
                     GAPYIIANNIDIERITEPLRIFDGTAREAAKGFPANLNVAVALSLAGIGADRTKLEIW
                     ADPTVTRNIHRIEVDSDSARFSMSIENIPSENPKTGRITALSVIACLRKQRASLRVGT
                     "
     gene            5623..6378
                     /locus_tag="VN968_10240"
     CDS             5623..6378
                     /locus_tag="VN968_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29652.1"
                     /translation="MIQTIEAVNGGYRFMPGVSQYSCGIAALAGFGIERISFSNVVPL
                     RQGFEKVAEIIKAAGRPLTAFGACELRSPAPFTEDGFKAFNETYIKTLVEWGVMRDGV
                     NPVARSNVCPEIDPPSEPGFHAFSFTSAALGAPASFVISGSGESVEGKANYRDHTVRL
                     GDTSPEGMLEKAKWVLDEMERRMSAFSGDWRQTTAVQLYTVRNVFPFLEGELGKRGVL
                     RHGLTWHFNRPPVVDLEYEMDCRRVHIERVIEV"
     gene            6504..7124
                     /gene="pncA"
                     /locus_tag="VN968_10245"
     CDS             6504..7124
                     /gene="pncA"
                     /locus_tag="VN968_10245"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HXL29653.1"
                     /translation="MQIRDDDLLLVIDVQTDFCPGGALAVADGDAVVPIINRLTERFS
                     HVALTQDWHPSGHSSFATSHPGSAPFQTIRMPYGEQTLWPDHCIQGAPGAAFHPQLVT
                     ERAELVIRKGFRPEIDSYSAFYENDRRTPTGLAGYLRERGLRRIFLAGLATDYCVYYS
                     AVDARRLGFDTVLIEAGCRAIDLAGSLDAAWAAMATAGVQRVNDLN"
     gene            7430..>8889
                     /locus_tag="VN968_10250"
     CDS             7430..>8889
                     /locus_tag="VN968_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL29654.1"
                     /translation="MSALALWVGLATPALAAGKTITAVMHSDLRIIDPGFTTAYITRD
                     HGYMVYDTLLATDSNFKIQPQMADWKVSDDKLTYTFTLRDGLKWHDGAPVTAEDCVAS
                     LKRWGRNDSMGQKLMDFTASIEASDEKTITLKLKEPYGLVLESIGKPSSYTPFMMPKR
                     LAETPPGQQIKEQIGSGPFKFVQAEFQPGVKAVYEKNVDYVPRKEPPSWTSGGKVVKV
                     DRVEWITMADAQTAVNALQSGDIDLMENPPFDLLPVLTANKDLKIETLNKLGFQTVGR
                     MNFLYPPFDNVKVRRAALMALNQKDVLDALVGDPKYYRICGAVFVCDTPLATEVGSET
                     LVKGNGMAEAKKQLAESGYDGTPVVIMAPSDVVTLKAQPIVSAQLLREAGFKVDVQAA
                     DWQTVVARRASQKPPKEGGWNMFFTNLTGADVVNPIANVQISGRGKNGGWFGWAEDAK
                     IEQMKDAFARSSSPEEQKKIAADIQKEAYEQVIYIPL"
BASE COUNT         1697 a         2670 c         2778 g         1744 t
ORIGIN      
        1 gccggtcatg ggtttcgaga tcgccggcga cccaaccgcc accatggaaa aacaccacgg
       61 tcggcgattt ttcgcgtgag acgcggtaga cgcgcgcggc aagcggcccg gccgcgccct
      121 tcaccttgat atcgtccgcg ctagcgaccg gcggcggcgg aatggcggcc cgcgccgcgg
      181 ccagcgcgcg tagcgcctcg cgggcgcttt gcggggtcat ggtttcggga tcgcgcagcg
      241 gcagcagcgg aatgatgatt tcgatgtcgg gatcgagagg tgcggtcatg ggccctccag
      301 gcgccggcaa ctacgcgagc gcaggttccg ctcgccgctt cggccatttg tcgtttgcga
      361 atggttttcg gattgaaccc acggacgtgt caatgcccga cgatctccgg cacccgggtt
      421 gccggcggcg tgttgcggct gcgctgggtg cggacgatgc cgtcgatgat ggtcatgccg
      481 atgccgggga gatcgccgag ctgcacgctg tcgaggatgt tcttgccggg cgaatgctgc
      541 gccttgtcca tgatgacgaa gtcagccgag cggccgacct cgataatgcc gcagtccagc
      601 gcgcgcatcc gcgcggtgtt gccggtcgcg aagcagaacg cgatttccgc cggtatctcg
      661 ccgagcgatg acagcagcga taccatccgc aagatgccca gtggctgcac gccggaaccg
      721 gccggtgaat cggtgccgag aatgacgcgg ctgagatcgc ccatctcgcg cgcgatccgc
      781 aaggtgtaga gcgcggagcg ctcgttgcca ttgtgaacca gctccagacc gcgcttgcag
      841 ccctcgcaga tgcagcggat ctggtcatcg ggcagggcgg tgtggccgcc attgatgtgg
      901 ccgatcacgt cggtgtcggc ttccagcacc acgtccttgt cgatcaggcc ggagccagga
      961 atcgatgggc ctccggtgtg gatggtgctt tgaattccat acttgcgcgc ccatcccacc
     1021 atcttgcgcg cggtcggacc atccttgacg ccgccaaggc cgacctcgcc gagcaacttg
     1081 acgccggccg ccgctagttc cctgaagtct tcctcgacca tctcgttttc gatcacgggg
     1141 gcgccggcat ggacctttac gccgccgggg cggagcgtcc agaacgagcg ttgtgcaaaa
     1201 atcgccatcg ccttcaggcc gacgacgtcg cgtggacggc cgggcatgtg gacctctcca
     1261 gcggaaatca tggtggtgac gccgccgtgc agaaagctgt cgatccagtt gatttgattc
     1321 tgccgcggcg tccagtcgcc ggccacggga tgcacatggc tgtcgatcag gcccggtgcg
     1381 acggtcgtgc cgttggcatc gacgagggtg gtggcgcctt cggcgtcgat gtccttggcg
     1441 cgtccgatcg ccgtgatcct gccgttctcg gccagaatcg tatcggcatc gaggatcggc
     1501 ttctccatcg cgcccgagag cagcaggccg atattgcgga tcaccagcct ggtggggccg
     1561 gcgccctggg gttcatcatg ggccatggat ttattccttt ttgctcaatg ccttcgcgat
     1621 cgtggacggc aacttgagcc cgacttgacg ccgggatcaa gttggaatat tcgtttgtat
     1681 acaaatgaac tgtggattgc gatcggcagc cccgcaggcc cggggatttt cgaccgcccg
     1741 cccttactcg gatcaagcag aatgagcagc ttcaatcagg aaagcgttct cagcgtccat
     1801 cactggaccg ataatttgtt tagctttacg accacccgcg acccgtcgtt ccgtttccgc
     1861 aacggcgaat tcacgatgat cggcctcaag gtcggcgaga agccgctatt gcgcgcctac
     1921 agcgtcgcca gcgccaatta tgaagaccgg ctcgaattct tttcaatcaa ggtgccggac
     1981 gggccgctga catcgcgtct gcagcatctg aagcaaggcg acgagatcat cgtcagccgc
     2041 aaggcgaccg gcacgctcgt gatcgacaat ctggaggacg gccgcaatct gtatttgatc
     2101 ggcaccggca ccgggctcgc gccgttcctc agcgtgatca aggacccgga gacttacgag
     2161 cgtttcgaaa aggtggtctt gctgcacggc tgccgtcgcg tcgccgaatt ggcctatggc
     2221 gagatgatca ccgagaacct gcctaaggac gaattaatcg gtgaactggt ccgcaaccag
     2281 ttgatctatt atccgaccgt gacgcgcgac ccgttccgca accgcggccg gatcacggac
     2341 ctgattactt caggcaaact gttcagcgac gcaggccttg ctccgctgga atctgcacat
     2401 gaccgcgtga tgatctgcgg cagccccgcg ctggttcatg acacgcgagc gctgttatca
     2461 ggcaaagatt ttgtcgaagg caatcacggc gagccgggac agttcttggt cgagaaggca
     2521 ttcgcggaac gctaaagcgc ggcgcacgtg cactcgttta tgcgagagtg atggccgtta
     2581 tcttgccgga caaccgcccc ggttttgatc caggcctcga caagtgcgcc agcttccttc
     2641 tgcgcgccgg gagcaggggt gctgccgaag ccaggcgccc acgccgagcc gaccagggtg
     2701 tcggtaccga tgtgatagat gaggtcctcg acttttcgtc cgccgttgcg gaccgggtcc
     2761 ttgatctgag cgcaaatctc ggcgagcgat ttgccttccc acgccatctc gcgcggcgcg
     2821 aggtgccata ccgggtgacc cggcacgcgg ccgggctcga agttggcgtt ctggcgacac
     2881 gacatgcaag tcatatatcg gctggtctgt tgcgaaatga ttgatgcagt ttgtaaaatg
     2941 aggtggcgtt ttcaagcggc gagcagtccg tgcggtcggt gacccaccct ccgatttgtc
     3001 aggttattgc ctggccaggg cctagccggg catcggtcgt catgctttga tcaagctcag
     3061 ccgaatgtaa tgaccgggga gtgttttgaa tggcgggcgc ctcgaacctc gatcggtgcg
     3121 ttgatgacgg cggatttcgc tgacgacgat gttgatgaat aacaagaccc aacccgccga
     3181 tcgcggtgcg gcgatctaca aggcgctctg gcacgccatc atcgagcggg cgcttcagcc
     3241 cggcgcgaaa cttcccgaag atgcgatcgg cgagaaatag gcctcttgga tgtcattcga
     3301 tacgattttg cgcaatggat gcatcgccgg ccgcgagcac gaaccggtcg atatagccgt
     3361 gcgcgatggc cggattgccg ccatcactcc gcggtcgaac caggcaaccc aggacgcagc
     3421 ctgcgatatc gcgcttgacg gaaagcttgt gatcccgggt ttcgtcgaga cccatatcca
     3481 tctcgacaaa tcatgcatct ccgatcgctg cagctgccgc accggcacct tgcaggaagc
     3541 gatcgagagc gtcgctgccg caaagcttgc cttcaccgag caggacattt acgcgcgagg
     3601 gcggcgcacg ctggaaaaag cgatcgtgca gggcacgacg gggatgcgcg cccatgtcga
     3661 agtcgatccg cggatcggac tgaaaggctt tcgcgccgtc agccagctga agcgagattt
     3721 cgcctgggcg gtggatattg aaatctgcgt tttcccgcag gaagggctgc tcaacgatcc
     3781 cggctgcgac gaattgctgg tccaggcttg cgatgatggc gctgatctga tcggcgggtg
     3841 cccctacacc gacaacgatc cgcacgggca aatcgcgcgc atcttcgaac ttgcgaaacg
     3901 ttacgatctc gacatcgacc ttcatctcga tttcgatctt gatccgtcct ggatgcacct
     3961 cgatgaagtt tgccggcaga ccgcgaagta cggctggggc gggcgcgtcg ccatcggaca
     4021 cgtcaccaaa ctgtcggcgc ttgaccatgc cgcgctggaa aagatcggcg tgaaattggc
     4081 caatgcgggt atagcggtaa ccgtgttacc cgcgaccgat ctgtttttga tgggccgcgg
     4141 cgccgagcgt tcggtgccgc gcggggtggc gccggcgcac cgcctgctcg ctcagggcgt
     4201 cacctgttcg cttgccacca acaatgttct taatccgttt acgccgtttg gcgattgctc
     4261 gctgctgcgg atggcaaacc tctatgccaa tatcgcgcag ctcggacggc ccgacgagct
     4321 gcaggcgtgt atggacatgg tcacgctgtt gccggcaaag ctgatgaatc ttggggatta
     4381 cggcattgcc gttggcaatc cggccgatat cgtcgtgctc gattgcgccg accgggtgtc
     4441 ggcggtcacc gaactggcgc aaccgctgct ggcaatgaag cgcggacgga taagcttttc
     4501 ccggccgacc gccaccctct attggccagg cgcaacgccg tctttggtaa aagacgactt
     4561 tggagcaggt ccgttcgccg acgcgaggtc tctcgcccgg tcgctaccgt agttgtttgc
     4621 caggattaag cgaggcccgg cgcgcggaac gctgtgacgg tgctgccatc ggaggatcga
     4681 gacgctaatg gccgatttat caaagggcgt accagccgac ttgcgcgttg ctattgccgg
     4741 gttagggccg atcggaaagc gggtagcgga agcgctggac cggggaatag acggattggc
     4801 tcttgtcgct gtgtcggcac aacatcccga gaagcaccga agctggcttt ccggcctgac
     4861 gagaacgccc gcggtcttgc cgatcgatgc gctctgcgac gtcgcggata tcgtcatcga
     4921 gtgcgcccca ggcaagctcg tcagatcaat cgttgaaccg ttcgcaacaa gcggaaagac
     4981 tgcggtggtt cttagcgcgg gcgcgctgct cgaccacgag gacctcatcg agattgccaa
     5041 gcgaaatggc ggccagatcg tggtccccac gggtgcgctg attggcctcg acgccgtcac
     5101 cgcggcggcg gtcgggacca tccattcggt tcggatggta acgcgaaagc cgatcgccgg
     5161 gctcgtcggc gcgccttaca ttatcgcgaa caatatcgat atcgagcgaa ttaccgagcc
     5221 gctcaggata ttcgatggta cggcgcggga ggcggcaaag ggatttccgg cgaatctgaa
     5281 tgttgcggtc gcgctgtcgc tggcggggat aggcgctgac cggaccaagc tggaaatctg
     5341 ggccgatccg acggtgacac gcaacattca tcgcattgag gtggactccg actctgcccg
     5401 cttttccatg tcgatcgaga atataccgtc agaaaatcca aagacgggtc ggatcaccgc
     5461 gctttcggtt attgcctgtc tgcgcaaaca gcgagcatcg ctccgtgtcg gaacttgaat
     5521 cacgggcaaa tgccattcaa ccgaagcatc tgatttcagc ttttattcgt actgcggatt
     5581 cagacttgcg aacagcttca ccgcgaaatc aacgagacat ctatgatcca gaccatcgag
     5641 gcggttaatg gcggctacag gttcatgccg ggtgtcagcc agtattcgtg cggtatcgcg
     5701 gcgcttgccg gcttcggcat cgagcgcata tccttctcga atgttgttcc attaaggcag
     5761 ggttttgaaa aggtcgcgga gattatcaag gctgccggcc ggccgctgac ggcgttcggc
     5821 gcctgcgagc tgcgttcgcc cgcgccgttt acggaagacg gttttaaggc tttcaacgag
     5881 acctatatca aaacgctggt cgaatggggg gtgatgaggg acggcgtgaa tccggtcgcg
     5941 cgcagcaatg tctgtccgga aatcgacccg ccttccgaac cgggatttca tgccttctcg
     6001 ttcacgtcag cggcgctggg cgcgccggcg tcgttcgtca tttccggcag cggcgagtcc
     6061 gttgagggca aggcaaacta tcgcgatcat acggtccgcc tcggtgacac cagcccggag
     6121 ggaatgctgg aaaaagccaa atgggtattg gacgaaatgg agcggcgcat gagcgcattc
     6181 tccggcgatt ggcgtcagac tacggcggtg cagctctaca ccgttcgcaa tgtgtttcca
     6241 tttctggaag gcgaactcgg caagcgcggc gtgctgcgcc acggcctgac ctggcacttc
     6301 aatcgcccgc ccgtggtcga tctggaatat gagatggact gccgccgcgt ccacatcgag
     6361 cgcgtaatcg aagtttgacg tttcgtcctc ttattgccaa tctgcattca gacatccgcc
     6421 ccttcagctt ccggttgccg gatcaaagct caacgcacct tgctaggggc aacgtttggg
     6481 gagcaacgat aggcaggttc acaatgcaaa tccgtgacga cgatctactg ctggtaatcg
     6541 acgtgcagac cgatttttgc cccggcggcg cgcttgcggt cgccgacggc gatgccgtgg
     6601 taccgatcat caatcgcctc accgagcgct tcagtcatgt cgcgctgacc caggattggc
     6661 atccgtccgg tcacagctcc tttgccacat cgcatccggg ttctgctccc tttcaaacca
     6721 tccgcatgcc atatggagag caaacgctct ggcccgatca ctgtatccag ggcgcgccgg
     6781 gcgccgcctt tcacccgcaa ttggtgacgg agcgggccga gttggtgatc cgcaagggat
     6841 tccgccccga aatcgattcc tattccgcgt tctatgaaaa cgaccggcgt acgcctaccg
     6901 gtttggctgg ttatttgcgc gagcgcggtt tgcggcggat cttcctggcc gggctcgcga
     6961 ccgactattg cgtgtattac tccgcggtgg acgcgcgccg gcttggcttc gacaccgtgc
     7021 tcatcgaggc aggctgcagg gccatcgatc tggcgggctc gctcgatgcc gcctgggcgg
     7081 cgatggcgac ggccggcgtt cagcgcgtca acgatctcaa ctagcgcgcg ttccgcaaaa
     7141 gcgggccctc cgacttgcat acgaggggtg gacactggtt ccgcctcgcc atcaagtcgg
     7201 cgcagattgc gcagatttat cagcggtaaa atgcgggcaa gtagatccgg tctttcttct
     7261 ccggtcgcgg cacgtcgcaa aagggccata accggaaaat cgtgaccctt tcaaagacta
     7321 gggacttgga atagtttttg gcgttgccag gagggacgcc gtgaaacttc ctcgccgacg
     7381 ttttttgtat ctggccgcag gcgctgccgc gctccctgtt cggcttaccc tgtcggccct
     7441 tgcgctctgg gttgggctgg cgacgcccgc gctcgccgcc ggcaagacca tcaccgcggt
     7501 gatgcactcc gatctgcgca tcatcgatcc cggcttcacc accgcctaca tcacgcgcga
     7561 tcacggctac atggtctacg acacgctgct ggccaccgat tccaatttca agatccagcc
     7621 gcagatggcg gactggaagg tctccgacga caagctcacc tacaccttca cgctgcgcga
     7681 cggcctgaaa tggcacgacg gcgcgccggt cacggcagag gattgcgtgg cgtcgctgaa
     7741 gcgctggggc aggaacgaca gcatgggcca gaagctgatg gatttcaccg ccagcatcga
     7801 ggcgtcagac gaaaagacca tcacgctgaa actgaaagaa ccctacggcc tggtgctgga
     7861 atcgatcgga aaaccgtctt cctatacgcc gttcatgatg cccaagcggt tggccgaaac
     7921 accacccggc cagcagatca aggagcagat cggctccggc cccttcaagt tcgtgcaggc
     7981 ggaattccag cccggcgtga aggcggtgta cgagaagaac gtcgactatg tgccgcgcaa
     8041 ggagccgccg agttggacct cgggcggcaa ggtggtgaag gtcgatcgcg tcgaatggat
     8101 cacgatggcg gacgcgcaga ccgcggtgaa cgcgttgcag tccggcgaca tcgatctcat
     8161 ggagaatccg ccgttcgatc ttctgccggt gctcaccgcc aacaaggacc tgaagatcga
     8221 aaccctgaac aagctgggct tccagacggt agggcgaatg aacttccttt atccgccctt
     8281 cgacaacgtc aaggtccgcc gcgccgcctt gatggccttg aaccagaagg acgtgctgga
     8341 tgcactggtt ggtgacccca aatattatcg catatgcggc gccgtctttg tctgcgatac
     8401 gccgcttgcc accgaagtcg gctcggagac gctggtcaaa ggcaacggca tggcggaagc
     8461 caagaagcag ctcgccgaat ccggctatga cggcacgccg gtcgtgatca tggcgcccag
     8521 cgatgtcgtg acgctgaagg cgcagccgat cgtatcggcg caattgctgc gcgaggccgg
     8581 cttcaaggtc gacgtgcagg ccgccgactg gcagaccgtg gtcgcccgcc gcgccagcca
     8641 gaagccgccg aaggagggag gctggaacat gttcttcacc aacttgaccg gcgccgacgt
     8701 ggtgaacccg attgccaacg tgcagatcag cggccgcggc aagaatggcg gctggttcgg
     8761 ctgggccgag gacgccaaga tcgaacagat gaaagacgcc ttcgcgcgct cgtcgtcacc
     8821 ggaggagcag aagaagatcg ccgccgacat ccagaaggaa gcctatgaac aggtgatcta
     8881 catcccgct
//
LOCUS       DATNOI010000263         3320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2343057,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000263 DATNOI010000000
VERSION     DATNOI010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2343057"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VN968_10255"
     CDS             complement(<1..180)
                     /locus_tag="VN968_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29655.1"
                     /translation="MSHRVAAFYQFAALPDFRELREPLRAICAEFRLKGSMLLAHEGI
                     NGTLAGSAEGIAALVE"
     gene            447..2033
                     /gene="ggt"
                     /locus_tag="VN968_10260"
     CDS             447..2033
                     /gene="ggt"
                     /locus_tag="VN968_10260"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXL29656.1"
                     /translation="MRNFHLAGRSTVHAQNAMVATSHPLAALTAIEVLRSGGTAADAA
                     VAACALLGVIEPQSTGIGGDCFALVQPRGEGKIVSYNGSGRAPQAATPEWYLERNIRA
                     IPLTSAHAVSIPGAIDAWATILRDHGKLGLDRLLQPAIKAAEEGYVVAPRIAFDWKNQ
                     FDKLKNGTNTERYLLPHGKPAAVGDVIRQPELGQTLRAIAKSGRDAFYKGAIAEDMVE
                     TLRGIGGLHTLDDFATHTTEITTPIGTMYKGHDVWQCPPNGPGITMLVMLNILARFDL
                     TKFAGLSVERFHLEAEAARAAYMMREQYIGDPSHVDVDVVRILAKEFAEEHASKISMD
                     RLRDLPNVAPPMNPSTVYITVVDSNRNVCSFINSIAHSFGSAIVSNKTGILLQNRGGG
                     FRIQPGHPNCIAPGKRPLHTIIPSLVTKAGRSVMPFGVMGGQYQPVGQVHVLTNMLDY
                     GCDVQEAIDMPRGLHYEGVYQLEDGVPADIVEGLNKLGHQTTSVVGPLGGGQAIWIDW
                     DKGTLTGGSDPRKDGCALGY"
     gene            complement(2126..2437)
                     /locus_tag="VN968_10265"
     CDS             complement(2126..2437)
                     /locus_tag="VN968_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29657.1"
                     /translation="MDGITTRAMALILDFLFVDFRTGFVNHDHASTLVAVVKVNGDLT
                     GDQVGGFPGVVLVLAIQSNRIFEPDTVSDVKMKNGHWLLLEVNAVKPSVFQDAVRRHQ
                     A"
     gene            2757..3224
                     /locus_tag="VN968_10270"
     CDS             2757..3224
                     /locus_tag="VN968_10270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXL29658.1"
                     /translation="MRRFQRAKMPMAAAIAMILAAVPLACVSTIAAAQTAGKTMTTAS
                     GLQMIDSTVGTGATPKPGQICVMHYTGWLYENGQKGKKFDSSVDRNEPFEFPIGQRRV
                     IAGWDEGVATMKVGGKRTLIIPPALGYGARGAGGAIPPNATLMFDVELLDVKG"
BASE COUNT          661 a         1007 c         1045 g          607 t
ORIGIN      
        1 ttcaaccagc gcggcaattc cctcggcgct gccggcaagc gtgccgttga tgccttcgtg
       61 ggccagcagc atgctgccct tcagcctgaa ctcggcgcag atcgcgcgca gcggctcgcg
      121 cagttcccgg aaatccggca atgcggcgaa ttgatagaag gcggcgaccc tgtgtgacat
      181 cgccgtggtt tatcaggagg gaggggacgc aatcaacaga tacggcgtcg tcccggcgaa
      241 ggccgggacc catacgccgt gccgtggcgg tcagaaaatg gagccaatgg cccttgctac
      301 aacgaacatc ggtggctgtg ggtaccggcc ttcgccggga cgacgcatgc tatgcgtctc
      361 caagcattgc tcatcacctc acgaatatgc catgtaaacc agcgcctgga gagcaacggt
      421 aaaacgacaa aagtgagtca tttggcatga ggaatttcca tctcgccggc cggtcgacgg
      481 tccacgccca aaatgcgatg gtggcgacgt cgcatccgct ggcggcgttg acggcgatcg
      541 aggtgctgcg ttccggcggc accgcagccg acgccgccgt ggcggcctgt gcgctgctcg
      601 gcgtgatcga gccgcaatcc accgggatcg gcggcgattg ctttgcgctg gttcagccca
      661 ggggggaggg caagatcgtc tcctataacg gctccggccg cgcgccgcag gctgcgacgc
      721 ctgaatggta tctcgagcgc aacatccgtg cgatcccgct gacatcggcg cacgccgtca
      781 gcattcccgg tgcgatcgat gcctgggcaa ccatcctgcg cgatcatggc aagctggggc
      841 tcgatcgcct gctgcagcct gcgatcaagg ccgcggaaga aggctacgtc gtagcacccc
      901 gcatcgcctt cgactggaag aaccagttcg acaagttgaa gaacggcacc aacaccgagc
      961 gctatctgct gccgcacggc aagccggcgg ccgtcggcga cgtgatcagg cagcccgagc
     1021 tcggccagac gctgcgggcg attgcaaaaa gcggccgcga cgcgttttac aagggcgcca
     1081 tcgccgagga tatggtcgag accttgcgcg gtatcggcgg gctgcacacg ctcgacgact
     1141 tcgccacaca taccaccgaa atcacgacgc cgatcggcac catgtacaag ggccacgacg
     1201 tctggcagtg cccgccgaac ggcccgggca tcaccatgct ggtgatgctg aacatcctgg
     1261 cgcgcttcga tctgacgaaa ttcgcaggcc tgagcgtcga gcggtttcac ctcgaggcgg
     1321 aagcggcgcg ggccgcctac atgatgcgcg agcagtacat cggcgatccc agccatgtcg
     1381 atgttgatgt ggtccgaatc ctggccaagg aatttgccga ggagcacgcc agcaagatca
     1441 gcatggaccg gctgcgcgat ctgccaaacg tggcgccgcc gatgaatccc tcgacggtct
     1501 acatcaccgt ggtggacagc aaccgcaacg tctgttcgtt catcaattcg atcgcgcact
     1561 cgttcggttc ggcgatcgtc tccaacaaga ccggcattct cctgcaaaat cgcggcggcg
     1621 gtttccgcat tcagcccggt caccccaatt gcatcgcgcc ggggaagcgg ccgctgcaca
     1681 ccattattcc gtcactggtc accaaggccg gccggtcggt gatgccgttc ggcgtgatgg
     1741 gcggacaata tcagccggtc gggcaggtgc atgtgctgac caacatgctc gactacggtt
     1801 gcgacgtgca ggaggcgatc gatatgccgc gcggcctgca ttacgaaggc gtctaccagc
     1861 tcgaggatgg tgtgccggcc gatatcgtcg agggcctgaa caagctcgga catcagacca
     1921 ccagcgtggt cgggccgctc ggcggcggtc aggcgatctg gatcgactgg gacaagggca
     1981 cgctcacagg cggttccgat ccacgcaagg acggctgcgc gctcgggtat tgaggttcga
     2041 cctcatcctg agcgttctgg tcgctttcga atcagactct caggatgaga ctgcaaccgg
     2101 cgggaacatt tgcgccgcga tggcctcagg cttgatgtcg acggaccgca tcctggaaca
     2161 cgcttggctt gacggcattg acctcaagga ggagccaatg cccatttttc attttgacat
     2221 cgctgacggt gtccggctcg aagatccggt tggactggat tgcaagaacg agcacgacgc
     2281 caggaaaacc gccgacctga tcgcccgtca aatcgccatt gaccttgacg acggccacga
     2341 gggtcgatgc gtggtcgtga ttgacgaagc cggttctgaa atctacaaaa aggaagtcaa
     2401 ggattagcgc catcgcgcgt gtcgtgattc cgtccatcga cgaaagtgtg ccgcggatct
     2461 cggtacggtt cgttcgctgg tgcgaccggt tcggagccag acctcattgt tttggttgaa
     2521 acgatgaggc gccgcatcgc ggccagttca ccgcccactt cttgaggttg tcatcacgca
     2581 gggcggatac ggaactcgca ggatcgcggc gaattatccg gggacgctat atctcgcatc
     2641 gccaacccat cgaagtgttc caggtccagt ccggtggatt tacgcctgtc aatgggtgga
     2701 aacgccgatg gcgttgggct agacccgctc cgatgattga gggaaggtgc ccaaagatgc
     2761 gacgttttca gcgtgcaaaa atgccgatgg cagcagcgat tgcaatgata ctcgcagctg
     2821 tgccgttggc gtgtgtatcc accatagcag cggcccagac cgcgggaaaa accatgacca
     2881 cagcttcagg cttgcagatg attgacagca cggtcggcac cggcgctacg ccgaagcccg
     2941 gtcagatttg cgtcatgcat tacaccggct ggctctatga gaacgggcag aagggcaaga
     3001 aattcgactc ttcggtcgat cgcaacgagc cgttcgagtt tccgatcggg cagcgcaggg
     3061 tgatcgccgg ctgggatgaa ggcgtcgcca ccatgaaagt cggcggcaag cgtaccttga
     3121 tcattccgcc cgcgctgggc tacggcgccc gcggcgccgg cggcgccatt cctccgaacg
     3181 cgacgctgat gttcgacgtc gagttgctcg acgtgaaggg gtaggcagcc gtcgtctaaa
     3241 ttcgtcatgg ccggcatcgc atagccctga agcttgtcgg aaccgatagc ttcgccgacg
     3301 ccctgcttgg ccttgccgat
//
LOCUS       DATNOI010000264         5696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_253281,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000264 DATNOI010000000
VERSION     DATNOI010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5696
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253281"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VN968_10275"
     CDS             <1..189
                     /locus_tag="VN968_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29659.1"
                     /translation="LGEMNPGQLWETTLDTNERSLLQVKIKEVDEADDIFTKLMGDVV
                     EPRREFIQDNSLAANVDV"
     gene            321..1745
                     /locus_tag="VN968_10280"
     CDS             321..1745
                     /locus_tag="VN968_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXL29660.1"
                     /translation="MTALEKIERGPIAAATSGNRLTVELTETLKLAVPMALTQLGQIA
                     MMTTDLALVGRLGDEAVAAAALAHTVFFVCFTFGMGLMSAVAPLAAQALGARNPRLVR
                     RSLRVGLWAGLLISLPMMAFPLYGEQILVTLGQAPATARLAQQYLFGLAWGITPALWF
                     LAIRGFMGAINRPEPGLWITLAAIPANAVLVYLLIHGALGLPRLELFGAGLGSTIIDF
                     GMLLAALWIAYGRRPFRKYRVLAHIWRIDWSLLRQLIVIGAPISIAFLLEYGLFAAAA
                     LLMGLIGTTALAAHQIALQVTAVLFMVPLGIGMAATVRVGHAVGRNDPAAVKRAGFVA
                     TLLGIVLVSALTLAVILGRYAVARFFFGEAAASAGDAVELTATLLAVGATFFVADGIQ
                     TVAAGALRGLNDTRVPLVFAAISYWLIGFTAAYGLAFRAGFGAVGVWIGLSCGTAVYA
                     ILLVLRFRRLASHPALAATAESWR"
     gene            1742..2215
                     /locus_tag="VN968_10285"
     CDS             1742..2215
                     /locus_tag="VN968_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HXL29661.1"
                     /translation="MRVHEVDSNVDSGALLAGAQFVDAYSIAIDGAALDARQAAEKML
                     ARLPRWIEVLLALRNFLVAPLGLKTSAPDGPGARDFIGILPVVRQTPDRIVAGFNDKH
                     LDFRLVVDVASAGHTRCITATTLVLTHNLLGRTYLAMIRPFHRLVVRSLLRQVAA"
     gene            complement(2238..3353)
                     /locus_tag="VN968_10290"
     CDS             complement(2238..3353)
                     /locus_tag="VN968_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HXL29662.1"
                     /translation="MTAFLKRLSARTRSRKAQLALALRVTLAAAAAYAIATALHLMLP
                     LWAVLTSLIVTQMSVGRSLKASRDYMLGTVGGAIYGGAIAVLIPHSGEIGLLALLVLA
                     VAPLAFIAAIHPSLNAAIVTAVIVLLVPEMRHANPLDSVIDRVMEVTVGAVTGLLVSF
                     LVLPSRAHSQIRVNAARLLDLLAAALSELLAGLTRGLDNDALHRIQDGIGAALVNLNA
                     TGAEAERERSARLSSGAETGPLLRTILRLRHDVVMIGRASVVPLPSDLQTRLAVPLAN
                     VSEAIATYLRSTADALRTGAGSPTLGPVEQALQAYAEEVAALRGEGLTRDLPGDAVER
                     FFALGFSLEQMRQNLKDLERCVAEWSDAPMAIPRAQD"
     gene            3487..4857
                     /locus_tag="VN968_10295"
     CDS             3487..4857
                     /locus_tag="VN968_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXL29663.1"
                     /translation="MADETATLAAYVVNLKFECIPHEVLERAKVLTLDFLGSAIRARR
                     DAESTPSLLKMLEALALDGRGESTVFGDSKTWTPAVAALLNGALGHSLDFDDTHADSS
                     LHASAPVVPAAFAVGELTGASGRDVLTAIVAGYEVCCRLGNALDPTSHYARGFHPTAT
                     AGTYGAAAAAAKLFGLSKEQIIAAFGVSGSQAAGSLQFLVNGAWNKRYQVGAAAMNGV
                     IAATLARNDFVGATESIEGKHGLLVGYSDNAHPDKAVAGLGKIYETMKIGVKPYPSCR
                     YTHAALDALIAMRREHNLTPDQIKRVEIGLHRNGITLTGDAATKRHPTSIVGGQFSMF
                     FTGSVALDQGRFGWDDYDRLGDATIDALADKFDVVQDDRLEVGRSHPFGARVSITTED
                     GVHERLHADPSGEPNSFPDPQAMAQKFLTLARPVLNGRADELANAILSLERFDRVEAA
                     THLGRQ"
     gene            complement(4868..5446)
                     /locus_tag="VN968_10300"
     CDS             complement(4868..5446)
                     /locus_tag="VN968_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL29664.1"
                     /translation="MLPPLKAGVTLAQPNGLVIDTERLKLRQWCGADVAPNAAMLSDP
                     GTARFIAADGKPPNSELAGWRNAAVMAGHWALHGAGMFVVEEKSSGKFVGRVGPWFPP
                     GWPGFEVGWGIAREFRGKGYALEAARASIDWSFATFDLDRILHCIDCENTPSQAVAQR
                     LGADKQHEIDLFGHVADVWVTWRDRWTTQLRK"
BASE COUNT          982 a         1892 c         1797 g         1025 t
ORIGIN      
        1 ctcggcgaga tgaacccggg tcagctctgg gaaaccaccc tcgataccaa cgagcgctcg
       61 ctgttgcagg tgaagatcaa ggaggtcgac gaggccgatg acatcttcac caagctgatg
      121 ggcgacgtgg tcgaaccgcg ccgcgaattc atccaggaca actcgctcgc cgccaacgtg
      181 gatgtgtgag caatcttccc cgtagagtcc gcaaatgcgc tttacgcgcc gtgtccgcca
      241 ttcgctgcac gtaacacccg tctgcgattg gagggtggcc acgcttcgct ttgcccactc
      301 cacgcgttag tctgccccct atgacagccc ttgagaaaat cgagcgcgga ccgatcgctg
      361 cggcaacgtc cggcaatcgc ttgacggtcg agttgacgga gacgctcaag ctcgcggtgc
      421 caatggcgct gacgcagctc gggcagatcg cgatgatgac gaccgatctt gcgctggtcg
      481 gacgcctcgg cgacgaggcg gtcgccgcgg cggcgctcgc ccatacggtg ttcttcgtct
      541 gcttcacctt cggaatgggc ctgatgtcgg cggtggcccc gctggccgcg caggcactcg
      601 gcgcccgcaa cccgcgcctg gttcggcgtt cgcttcgcgt tgggctttgg gcgggcctgc
      661 tgatttccct gccgatgatg gcgtttccgc tgtacggcga acaaatcctt gtgacgctcg
      721 gccaggcgcc cgctaccgcg cgtctcgcgc aacaatatct gttcggtctg gcgtggggca
      781 tcacaccggc actgtggttc ctggcaatcc gcgggttcat gggcgcgatc aaccggccgg
      841 agccgggatt gtggatcacg cttgccgcga tcccggccaa tgccgtgctg gtctatctcc
      901 tgatccatgg cgcactggga ttgccgcggc tggaactgtt cggcgcaggt ctcggctcga
      961 ccatcatcga tttcggcatg ctcttggcag cactctggat cgcctatggc cgccggccgt
     1021 tcaggaaata tcgcgtactc gcgcacatct ggcgcatcga ctggtcgctg ctacggcaac
     1081 tgatcgtgat cggcgcgccg atctccatcg cgttcctgct ggagtacggc ctgttcgccg
     1141 cggcagcgct tttaatgggc ctgatcggga ccacggcgct agccgcgcat cagatcgctc
     1201 tgcaggtcac cgccgtcctg ttcatggtgc cgctcggtat cggaatggcg gccacggtgc
     1261 gggtaggaca cgccgtcggc cgcaatgatc cggcagcggt caaacgtgcg ggctttgtcg
     1321 ccacgctgct cggaatcgtg ttggtatccg ccttgacgct ggccgtaatt ctcggccgat
     1381 acgcggtcgc gcgattcttc ttcggcgaag ccgcggcgag cgctggcgac gccgtcgagc
     1441 tcaccgcgac gcttctggcg gtcggtgcga cgttctttgt cgccgacggc atccagacgg
     1501 tcgcagccgg cgcgctacgt ggcttgaacg acacgcgggt gccgctggtg ttcgccgcga
     1561 tcagctactg gctgatcgga tttaccgccg cctacgggct cgccttccgg gccggatttg
     1621 gcgctgtcgg cgtctggatc gggctgtcgt gcggaactgc cgtttatgcc atcctcctcg
     1681 tcttgcgctt ccgccggctt gccagtcacc cggcgctcgc ggcaaccgca gaaagctggc
     1741 gatgagagtc cacgaggtcg attcgaatgt tgacagcggc gcgctgctcg ctggcgccca
     1801 gttcgtcgat gcctacagta tcgcgattga cggtgccgcg cttgatgcgc ggcaggcggc
     1861 tgagaaaatg ctggcgcgct tgccgcgatg gatcgaggtg ctgctggctt tgcgcaattt
     1921 tctggtggcg ccgcttgggc tgaaaacgtc ggcaccggac gggcccggcg cccgagattt
     1981 catcgggata cttccggtcg tgcgccaaac gcccgatcgc attgttgcgg gctttaacga
     2041 caagcacctg gattttcgcc tggtggttga tgtcgcaagc gccggccata cccggtgcat
     2101 caccgcgacc acgctggtat tgacgcacaa cctgcttggc cgaacctatc tggcgatgat
     2161 ccggccattt caccggctcg tcgttcgcag cttgctgcgg caggtcgcgg cctgagagtt
     2221 tcgccgtctg atccggccta atcctgcgcg cgcggtatcg ccatcggcgc gtcagaccac
     2281 tccgcgacgc agcgctccag gtccttcaga ttctgccgca tctgttcgag cgaaaatccc
     2341 agcgcgaaaa atcgttcgac cgcgtcgccg ggcaggtcgc gggtcaggcc ctcgcctcgc
     2401 agcgctgcga cttcttcagc ataggcctgc agagcctgtt cgaccgggcc gagcgttggc
     2461 gaaccagctc cggtgcgcaa cgcatcggct gtcgaacgca gataggtggc aattgcctcg
     2521 ctgacattgg ccaacggcac cgccaggcgc gtctgcagat cggaaggcag gggcaccaca
     2581 ctcgcccgcc cgatcatcac gacgtcgtgg cgcagccgca atatcgtccg cagcagcggt
     2641 ccggtttcgg cacccgatga caaacgggcg gagcgctcgc gctcggcttc cgcgcccgtc
     2701 gcattcagat tgaccaacgc cgcgccaatc ccatcctgga ttcgatgcaa ggcgtcgttg
     2761 tcgagcccgc gcgtcaggcc cgccagcaat tcggagagtg ccgccgcgag caagtcgagc
     2821 agccgcgcgg cattgacgcg gatctgactg tgcgcccgcg acggcagcac caggaacgac
     2881 accaaaagcc cggtgaccgc cccgacagtg acctccataa cgcgatcgat caccgaatcg
     2941 agaggattgg cgtgcctcat ttccgggacc agcagcacga tcaccgcggt gacgatggct
     3001 gcgttcaggc tgggatggat ggcggcgatg aaagccagcg gcgcgaccgc gagcaccagc
     3061 aacgcaagca gtccgatctc cccggaatgc gggatcagca ccgcgatggc gccgccatag
     3121 atcgcgccgc ccacggtccc gagcatataa tcacggctcg ccttcagcga tcggccgacg
     3181 ctcatctggg tgacaataag cgacgtcagc accgcccata gcggcaacat caggtggagt
     3241 gccgtggcga tcgcgtaggc ggcagcagcc gccagggtga cccggagcgc cagggccagc
     3301 tgcgccttgc gggaccgcgt ccgggcggac aggcgtttca agaaggcggt catgtgcccc
     3361 attccggaat gttatagacc aaacccgtta tagacgacct taggcatggc tgcttcccat
     3421 cgcccgcagc cggcttgaaa gccggaatca ggccgcctac cctgtgcaga acagcgagga
     3481 aacacgatgg ccgatgaaac cgcaacgctc gccgcctacg tcgtcaacct gaaattcgaa
     3541 tgcattccgc acgaagtgct ggagcgtgcc aaggtcctga cgctcgattt tctcggcagc
     3601 gccatccgcg cgcgacgcga cgcggaatcg acgccgtcgc tgctcaaaat gctcgaagcg
     3661 ctcgcgctcg atggtagggg cgaatccacc gtgttcggcg acagcaaaac ctggacgccc
     3721 gcggttgcgg ccctgctcaa cggcgcgctc ggccattccc ttgatttcga cgacacgcat
     3781 gcggattcct cgctgcatgc gagcgcgccg gtggtgccgg ccgctttcgc ggtcggcgag
     3841 ttgaccggcg catccggccg ggacgtgctc accgccatcg tcgccggcta cgaggtatgc
     3901 tgtcggctcg gcaacgcgct cgatccgacc tcgcattatg cgcgcggatt ccatcccacc
     3961 gccaccgccg ggacctatgg cgccgccgcg gcagcggcaa agctgttcgg gctgtcgaag
     4021 gagcagatca tcgcggcgtt tggtgtctcc ggcagccaag ccgcgggctc gttgcagttc
     4081 ttggtcaacg gcgcctggaa caagcgctac caggtcggcg ccgccgccat gaacggcgtg
     4141 atcgcggcca ccttggcgcg caacgatttc gtcggcgcaa ccgagtcgat cgagggcaag
     4201 catggcctgt tggtcggcta cagcgacaac gcccatccgg acaaggcggt cgccggcctc
     4261 ggcaaaatct acgagaccat gaagatcggc gtaaaaccct atccgagctg ccgctacacc
     4321 cacgccgccc tcgatgcgct gatcgcaatg cggcgggagc acaatctcac ccccgaccag
     4381 atcaaacgcg tcgagatcgg cctgcaccgc aacggcatca cgttgaccgg cgacgccgcc
     4441 accaagcggc acccgacctc gatcgtcggc gggcagttct cgatgttctt caccggctcg
     4501 gtagcgctcg atcagggccg cttcggctgg gacgactatg ataggttggg cgacgctaca
     4561 atcgatgcgc tcgccgacaa gttcgacgtg gtgcaggacg atcggcttga ggtcggccgc
     4621 agccatccgt tcggcgcgcg cgtgagcatc acaaccgagg acggcgtgca cgaacggctc
     4681 catgccgacc cctccggcga accgaactcg ttccctgacc cgcaggcgat ggcgcagaaa
     4741 ttcctgacgc tcgcccgccc ggtgctgaac ggccgcgccg atgagcttgc caacgcgatc
     4801 ctgtcgcttg agcgtttcga tcgggtggaa gccgcgaccc acctcggacg gcagtaggcg
     4861 tagcggttta ctttctgagt tgcgtcgtcc accggtcgcg ccaggtcacc cagacatccg
     4921 cgacatggcc gaacaggtcg atctcatgct gcttgtcggc accaagtcgc tgcgccaccg
     4981 cctgagacgg cgtgttttcg cagtcgatgc agtggaggat acggtccaga tcgaaggtcg
     5041 caaacgacca gtcgatcgac gcccgggccg cctcgagcgc gtagcccttg ccgcgaaact
     5101 cgcgggcgat gccccagccg acctcaaacc ccggccagcc cggcggaaac cagggtccaa
     5161 cccgcccgac gaattttccg cttgattttt cctcgacaac aaacatgccg gcgccgtgca
     5221 gcgcccaatg tccggccatc acggccgcgt tgcgccatcc cgccaattcg ctgttgggcg
     5281 gcttgccatc ggcggcgatg aatcgcgcgg tgccgggatc ggacagcatc gcggcattgg
     5341 gcgcgacgtc ggcgccgcac cactgccgca gtttgagacg ctccgtatcg atcacaaggc
     5401 cgttcggttg ggcaagcgtg acgccggcct tgagcggggg taacatcggt ttccgtcctc
     5461 acaattactt ggcaagcatg gaagtgctta ccgccgtcat tgtgaacagc gcaagcgacg
     5521 gtgagatgat atatcgccgc tgggacgggg ctgggcaatt gctgctatga tatcaacgaa
     5581 aatcatggag gcctcatgag ctggatgccc accaacgatc ccgtgctcgg cgatcccaaa
     5641 tcctgcgacg cgctcgaact ggttatcgtg ccgcgcaccc gcgacctcgg cgatgg
//
LOCUS       DATNOI010000265        12719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1124851,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000265 DATNOI010000000
VERSION     DATNOI010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12719
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124851"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(12..72)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(186..527)
                     /locus_tag="VN968_10305"
     CDS             complement(186..527)
                     /locus_tag="VN968_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HXL29665.1"
                     /translation="MKIPKDAVLLRIFIGESDEFGGRPLHEAIVLKAREMHLAGATVL
                     RGPMGFGQSSLLHTAKILRLSADLPLIIEIVDAEEKIRELLPVLEEMIGSGLVTLEKV
                     QVLQYGKNKND"
     gene            complement(530..1051)
                     /gene="crcB"
                     /locus_tag="VN968_10310"
     CDS             complement(530..1051)
                     /gene="crcB"
                     /locus_tag="VN968_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HXL29666.1"
                     /translation="MKGGSAMQDSTSPTSKADKRTTKHPPSRPRRPTWWQTIWDTAGS
                     YISVALGSVVGGVARFLVSVLFVSEFGNGFPWSTLFVNVTGSFIIGFYAALTGPDGRL
                     LASPRQRQFVMVGICGGYTTFSAFSLETLRLVQSGNLQAALLNIGISVAGWLTAVWIG
                     HALATRINRLRGT"
     gene            complement(1048..1440)
                     /gene="crcB"
                     /locus_tag="VN968_10315"
     CDS             complement(1048..1440)
                     /gene="crcB"
                     /locus_tag="VN968_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HXL29667.1"
                     /translation="MDTLDGVVWVALGSVLGGIARFWLSGFVARRVGETFPWGTMVIN
                     VSGAFVIGVLAASAAGHGALAAVRPWQFAVTGFLGCYTTVSSFSLQTLALARDGEMAR
                     ASGNLLLSLLLCLAAVTAGFAAGSHVFG"
     regulatory      complement(1757..1819)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(1891..2859)
                     /locus_tag="VN968_10320"
     CDS             complement(1891..2859)
                     /locus_tag="VN968_10320"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HXL29668.1"
                     /translation="MSMPCLFKGRLSLPVIGAPLFIISVPDLVIAQCKAGVVGSFPAL
                     NARPPALLDEWLARIKQELAAHDQAHPDRLSAPFAVNQIVHRSNNRLDQDLALCEKHK
                     VPMVITSLGAREELNQAAHRWGGIVFHDVINQKFAHKAIEKGADGLILVSAGAGGHAG
                     EISPLAFVAETRQWFDGPIALSGAIANGRAIRAARILGADFAYIGSAFIATAEANAVE
                     GYKQMITTSSAEDIVYSNLFTGVHGNYLKPSIVAAGMDPDNLPESDASKMSFGTDASG
                     ERAKPKAWKEIWGSGQGVGSVGKILPAAELIARFKKEYDEAVDPPL"
     gene            complement(2994..3536)
                     /locus_tag="VN968_10325"
     CDS             complement(2994..3536)
                     /locus_tag="VN968_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29669.1"
                     /translation="MSMVAAGVGWPQTPPGPSSLPPSLGVQTPAQQAAPDRPAPAQGE
                     PAPSSAPPEREDNPGLINEMGKFFEKTLSILPPLKSPQETIEDLNSRARDAGDGLTSM
                     AKPSSMVTGRMGCPVAANGVPNCKAGADKLCQAKGFKEGKSLDTDAAEKCSAKVYLPG
                     YKRGPGDCRTENYVIRALCQ"
     gene            complement(3661..4242)
                     /locus_tag="VN968_10330"
     CDS             complement(3661..4242)
                     /locus_tag="VN968_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HXL29670.1"
                     /translation="MLKRIYNWCIDAAHKPYALWILGAVAFAESSFFPVPPDVMLIPM
                     SLARPERAWLYASVCTAASVLGGILGYAIGALLYDSIGHWLIQFYGLGGKVEAFRAGY
                     AEYGAWIILLKGLTPIPYKLVTITSGFANYNIWLFVALSVIARGGRFFVVAILLNRYG
                     EWIRIRIEKHLGLWVGLAAGVLVLGFIVAFRMI"
     gene            complement(4304..4705)
                     /gene="hisE"
                     /locus_tag="VN968_10335"
     CDS             complement(4304..4705)
                     /gene="hisE"
                     /locus_tag="VN968_10335"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HXL29671.1"
                     /translation="MSDSLERLYLAVIAAKDLDPATSRTARLFQRGPTKMAKKLAEEA
                     IEVVIDAVNGNTPAVIRESADLLYNLTVLWASAGVRPEDVWREMERREHLLGIAEKLP
                     KSVVKLPKAAPAPAVRRRIVALEGRSLRKRH"
     gene            complement(4832..5263)
                     /locus_tag="VN968_10340"
     CDS             complement(4832..5263)
                     /locus_tag="VN968_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29672.1"
                     /translation="MFARNALFSSLGRAAVMATVAAVALTTVEPSLALAGSVAAGKGV
                     SAAAATSGATDFSARRRYYRGGGGAAAAAAFAGIVGTGLAIAASQNRRDDYYYDSSPA
                     YYGGGPYYGGDAYYGGTPYYGGYGYRHSGVPYFNGHPNAGW"
     gene            5570..6532
                     /locus_tag="VN968_10345"
     CDS             5570..6532
                     /locus_tag="VN968_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL29673.1"
                     /translation="MSDVRQPVAIQFTLDRPIDAGAAPFVMAAARGMFASEGLAVTTN
                     IASGSQDAIARVAAGASDFAQVDINALIRFRDRPKAPPIKAVFVLFNQSPYAIIARKN
                     RGIHALSDIEGKNLGVGEGDLSIRLWPALARQNGIKTASVKQNSISAAVREPMLSAGQ
                     VDAVTGFSYLSGVNLRDRGVPADDLAVLRFADYGCEAYGFSVIVNPAFAAAKPEAVRG
                     FLRAVIAGTHLAIKDPARGADEVVSRMDDGSRDLELERLDTVIRDNILTSEVKRDGIG
                     GIDPARLERSIDQIAEDFKFQKRPSAKDIFDDAFLPPLDGRLIN"
     gene            6623..8428
                     /locus_tag="VN968_10350"
     CDS             6623..8428
                     /locus_tag="VN968_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015441 - ABC-type beta-glucan transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015775 - beta-glucan transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan ABC transporter ATP-binding protein/
                     permease"
                     /protein_id="HXL29674.1"
                     /translation="MSLLRLYTRVLHLLGKEARLGWILAGANLLLAAAQFAEPVLFGR
                     IVDVLSGNPSTGPLASSSAWPLLGAWAAFGLFTIGCSAAVALHADRLAHRQRQAVLTN
                     YFEHIMQLPLTFHTGTHSGRLMKVMLNGTDSLWRLWLAFFREHFAAIMSLVVLLPLAL
                     YINWRLAILLFALCVVFTVLTTLVVRKTYGMQSEVEAHYSDLSARASDALGNVALVQS
                     FVRIDAEVQGLRFVADKLLAVQMPVLSWWALVTVITRASTTITVLAIFTVGIALHDQG
                     ITSVGEIVMFVSFATMLIQKLEQVVSFINNVFMEAPRLQEFFHVLDAVPAVRDRPDAI
                     DAGRLSGLVEFKDVSFSYDGKRPAVEDLSFTALPGQTVALVGPTGAGKSTAIALLHRA
                     FDPQSGFIRIDGMDVRGLTLTALRRNIGVVFQEALLFNRSIADNLRVGKPDATDQEMR
                     IAAARAQALEFIERSERGFESNAGERGRMLSGGERQRLSIARALLKDPPILILDEATS
                     ALDAVTEAKLNAALDEVMKGRTTFVIAHRLSTIRNATRILVFDSGRVIESGTFDELVA
                     KGGRFAELARAQFMVQEPARASIHAKGTDSAALKI"
     gene            8660..10021
                     /locus_tag="VN968_10355"
     CDS             8660..10021
                     /locus_tag="VN968_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXL29675.1"
                     /translation="MPCKSSSCWIVLLAALAIVAPRVVQAEALLVVEADTGKVLQADN
                     ATYPWYPASVTKLMTAYVTLKAVKDGHITLDTLFTVSPVAASQSPSKMGFRPGTQVTV
                     DNALKMMLVKSANDMAAVLAEGVGGSIDGFSAQMNQAAQRLGMTQSSYVNPNGLPADG
                     QITSARDLAILARAIIRDLPEYEYFVHIPSIRYGRKVTQNFNKLIGRYPGADGFKTGF
                     ICASGYNLVASATRNGKRLIAVVLGASSGQMRAVRAAQLLERGFANNQLAWLRPSLGT
                     VDNLVPIDASPPNLRDEMCGGKHKRPASDEDEDAVASNASGPSTGETAITFFTAGLQP
                     PMARPSDLLAAEAAPSEPIPVYTGPTKTGPALIAAVAMDSEQQTPPHRGKKKSRVASK
                     KPDTGAEPAAAKHANAKPDGTPKVAEKPAKPKAVVKPKPAPKSTTSQANPVDQKAVTA
                     PHT"
     gene            10174..11856
                     /locus_tag="VN968_10360"
     CDS             10174..11856
                     /locus_tag="VN968_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXL29676.1"
                     /translation="MERIWLKQYPAGVPADVDVTQYTSLVELLEESFAKFSDRKAFIC
                     MDRSISYRDLDEMSLALAAYLQSKGLQKGARVALMMPNVLQYPISTAAVLRAGYTAVN
                     VNPLYTPRELEHQLKDSGAEAIIVLENFATTVQQVMARTAVKHVIVGSMGDLLGLKGM
                     IVNLVVRRVKKMVPAYSIPGAVAFNDAVVAGRSVRLNKPKLAPDDVAFLQYTGGTTGV
                     SKGATLLHRNILANVLQNDAWLQPALRKPPHVDQLLIVCALPLYHIFALTACFLLAMR
                     AGGVNLLIPNPRDVAGFIKELMKYQVNSFPAVNTLYNGLLHSPGFDKVDFSKLKTSFG
                     GGMATQKAVAEAWLKTTGCALSEGYGLSETSPTLTCNPADTDKFSGSIGLPVPSTYLS
                     IRDDDGNEVPLGEPGEICAKGPQVMAGYWNRPEETANVMTADGYFRTGDIGVMTPDGY
                     TKIVDRKKDMILVSGFNVYPNEIEEVIASHPGVLECAVIGVVDPRSGEAVKAFVVKKD
                     PNVTAEDIIKFCGTQLTNYKVPKQIEFRTSLPKTNVGKILRRELRDEKKAAA"
     gene            11883..12437
                     /locus_tag="VN968_10365"
     CDS             11883..12437
                     /locus_tag="VN968_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HXL29677.1"
                     /translation="MTGACDLAPADILAFWRDAGCERWYKRDDAFDAEVRSRFFELWQ
                     KAAAGELSSWEATDDGALALVIVLDQFPRNMFRDDVRAYSSDGLARAVADRAIARGAD
                     IGVEAGLLEFLYMPLMHSEQLADQQRCVELFRKAGGADNLKYAEDHAEIIRRFGRFPH
                     RNPVLGRPTTPAEQAFLDSDGFSG"
     gene            12546..>12719
                     /locus_tag="VN968_10370"
     CDS             12546..>12719
                     /locus_tag="VN968_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HXL29678.1"
                     /translation="MTIKVGDKLPEAKFRVMTAEGPQVKTTDDIFKGKKVALFAVPGA
                     YTGTCHKMHLPSIF"
BASE COUNT         2485 a         4070 c         3851 g         2313 t
ORIGIN      
        1 ctgctcgcag tggcgcagca ggagtcatca gcctcttcgg cggttttggg ggaccccatc
       61 cccgttttgg atatttcgac tttagcgggg aaatcagcga aaacaaggat caactggttc
      121 cagggctcga cccgcaatta aattgcattg gcgcgttgcc ggagcctcgc gttcatcccc
      181 acccatcaat cgtttttgtt cttgccgtat tgcagcacct ggactttctc aagcgtaacg
      241 agcccgctgc cgatcatctc ttcgagcaca ggcagcaatt cccggatttt ttcttcagca
      301 tccacgatct caatgatcaa aggcaagtcc gccgagaggc gcaggatctt tgcggtgtgc
      361 aacaggctcg actggccgaa gcccatcggt ccacgcaaca ccgtggcgcc ggcaagatgc
      421 atctcgcgcg ccttcaatac gatggcttca tggagcgggc ggccgccgaa ttcatcgctc
      481 tcgccgatga aaatgcgcag caacactgcg tctttcggta ttttcatttt caggttcctc
      541 gcaaacgatt gatgcgcgtc gcgagcgcgt gaccgatcca cacggccgtc aaccagcccg
      601 cgacggagat gcctatgttg agtaatgcgg cctgcaaatt gccggattgc acaagtcgca
      661 acgtctcgag actgaaagcg gaaaacgtgg tgtagccccc gcaaattccg accatcacga
      721 actgcctctg ccgcgggctc gcgagcaacc ggccgtcggg ccccgtcagc gcggcataaa
      781 acccgatgat gaaggagccc gtcacgttga cgaacaaagt gctccaggga aagccatttc
      841 cgaactcgct cacgaacaga acagaaacca gaaagcgcgc cacgccgccg accacgctgc
      901 ccagggcgac ggaaatatag gaacccgctg tgtcccagat tgtttgccac caggttggac
      961 ggcgcggacg agaaggcgga tgtttcgttg tccgtttgtc tgccttggag gtcggtgagg
     1021 tgctgtcttg catggcagag ccgcccttca cccgaacacg tgggagccag ccgcgaagcc
     1081 agcggtgacg gccgcgagac aaagaagcag cgaaagcagc aaattcccgg atgcacgtgc
     1141 catttcgccg tcacgcgcca gagccaaggt ctgaaggcta aacgaggaca cggtcgtgta
     1201 gcatccaagg aaaccggtca cggcgaattg ccagggtctc accgcggcaa gagccccatg
     1261 gccggccgcc gatgcggcga gaacgccaat gacgaaggca ccgctcacat tgatgaccat
     1321 tgttccccag ggaaatgtct cgcccacgcg ccgcgccacg aaaccggaga gccagaatct
     1381 cgcgatgccg cccaacacgc tgccaagcgc aacccagacg actccatcaa gtgtatccac
     1441 cgcatgcctc gacttctctt cgctcttcca ggcggctcag gtcgagcgct ggtaagccaa
     1501 tcatcgtgtc tgcgccccac ctcttgcgga attgcttcgc tcgtcggttg atccctgagc
     1561 gatcgccttc agcgtggcga ggcgtatgat ctcatcgtcc gttgcatttt ttccccgcca
     1621 acaagcgttc ctgtcaacag caacacccgt cggcacacca tctctggttg gcaaacgaca
     1681 cagcaacctt acttcgcgtg gagacggcca aggagcctga gcgggtgcat cgccatggcc
     1741 tgacccccgc attctcgaca cggcaggagt catcagccct tatcaggcgg ttttggggaa
     1801 ccccttcccc ttggataaga gaatactaga cgatgtcaaa ccagggatca atatcgaccg
     1861 acagcagccg gcccttcttg gccggcagca tcacagcggc ggatcgaccg cttcgtcgta
     1921 ttcctttttg aatcgcgcga tcaattcggc ggcgggcagg attttgccga cgctgccgac
     1981 gccctggccg ctgccccaga tttccttcca ggctttcggc ttggcgcgct cgcccgatgc
     2041 gtcggtgccg aagctcattt tcgacgcgtc ggactccgga aggttgtcgg gatccatccc
     2101 ggccgccacg atcgacggct tcaggtaatt gccgtgcacg ccggtgaaca ggttcgaata
     2161 gacgatatct tccgctgatg acgtggtgat catctgtttg tagccctcga ccgcgttggc
     2221 ttccgcggtg gcgataaagg ccgagccgat ataggcaaag tcggcgccga gtatccgcgc
     2281 tgcgcggatg gcgcggccgt tggcgatcgc gcccgacagc gcgatcggcc cgtcgaacca
     2341 ttgccgggtc tccgccacga atgccagcgg cgatatctcg ccggcatggc cgcccgcgcc
     2401 ggccgatacc aggatcagac cgtccgcgcc cttctcgatc gccttgtgcg cgaatttctg
     2461 gttgatcacg tcatgaaaaa cgatgccacc ccagcgatgc gcggcctgat tgagttcttc
     2521 gcgcgcgccg agcgaggtga tgaccatcgg caccttgtgc ttctcgcaaa gcgcgagatc
     2581 ctgatcgagc cggttgttcg accggtgcac gatctggtta accgcgaacg gcgctgacag
     2641 ccggtcggga tgcgcctggt cgtgcgcggc aagctcttgc ttgatccgcg ccagccactc
     2701 gtcgagcagc gccgggggac gcgcgttcag cgccggaaac gatccgacca cacccgcctt
     2761 gcactgcgcg atcaccagat cgggcacgga aataatgaac agcggcgcac cgatcacggg
     2821 taaagacaga cggcccttga acaagcaagg catggacatt ccgaaaaaat ccttttggtc
     2881 attcagggca agattcgtgg caaacgtgat gcgtcgatct aaagatgcca gacatcatac
     2941 caacaaggca atcgtaccga ggaggccagc cggccaacag cccgatcccg gcgctactgg
     3001 cacagcgcgc ggataacgta attttcggtc cggcagtcgc ccggcccgcg cttgtagccc
     3061 ggcagataca cctttgccga gcatttctcg gccgcgtcgg tgtccaggct cttgccctcc
     3121 ttgaagccct tggcctgaca gagcttgtcg gcgccggcct tgcaattcgg cacgccgttg
     3181 gccgccacgg gacatcccat gcgccccgtc accatcgacg atggtttggc catgctcgtc
     3241 aggccatcgc ccgcatccct ggcgcgcgag ttcaaatcct caatggtttc ctgagggctt
     3301 ttcaggggag gaaggatcga cagggttttc tcgaagaatt tccccatttc gttgatcagg
     3361 ccgggattgt cttcgcgctc cggcggagcg gatgacggtg caggctcgcc ttgcgcgggc
     3421 gcaggccgat caggcgcagc ttgctgggcc ggcgtctgga cgccaagcga gggtggcagc
     3481 gaagatggcc ccggcggcgt ttgcggccac ccgacgcccg cagcgaccat cgacatcgac
     3541 aatgccacgc ccgcggtcgc aacccgcgtc ctggatagcg cgataaggct aatggttcga
     3601 tcaggcatga cgtcgagcgt aacctgaagt agcaaaccgg cgaagtactc accgggattg
     3661 tcaaatcatt cgaaatgcaa cgataaaacc cagcaccagg acgcccgccg ccagcccgac
     3721 ccatagcccc aggtgtttct cgatcctgat ccggatccat tcgccgtagc ggttgagaag
     3781 aatagcgacc acgaaaaacc gcccgccacg cgcaatcacc gacaatgcga cgaacagcca
     3841 gatgttgtaa ttggcaaaac cggaggtgat ggtgacgagc ttgtagggga tcggcgtcaa
     3901 acccttcagc aggatgatcc acgcaccata ttcggcatag ccggcgcgga aggcctcgac
     3961 cttgcctcca aggccgtaaa actggatcag ccaatgtccg atcgagtcat acagcagcgc
     4021 gccgatggca tagccgagaa taccgccgag caccgaggcc gccgtgcaga ccgaggcata
     4081 gagccacgcc cgctccggcc gcgccagcga catcggaatc agcatcacat ccggcggcac
     4141 cggaaagaac gaactttccg cgaacgccac cgcccccaaa atccacagcg cgtagggctt
     4201 gtgcgcggcg tcaatacacc aattgtaaat ccgtttcagc atggcgcgat gaaccaccct
     4261 agagcggatt tgtccatggc gggacatgcc gattgaaggc ggtttagtgt cgcttgcgca
     4321 ggctgcggcc ttcgagcgcg acaattcgtc gccggacggc tggggccggg gccgccttgg
     4381 gcagcttgac cacggatttc ggcagcttct cggcgattcc gagcagatgt tcgcgccgct
     4441 ccatttcgcg ccacacgtcc tcgggacgca cgccggccga cgcccataaa acggtgagat
     4501 tgtagagcag atcggcactc tcgcggatga ccgcgggggt attgccgttg acggcatcga
     4561 tcacgacttc gatggcctct tccgccagtt tcttggccat cttggtcggg ccgcgctgaa
     4621 acagccgtgc ggttcgcgac gtggccggat cgagatcctt ggccgcgatc accgccagat
     4681 aaagccgttc gagcgaatca ctcatcctgc caaactaatc caaaccagtg gccagcgtgt
     4741 taacggccgg ttaaccgagg ccgtaaaccg gaaagaaaat ccgccggcca cccaagcagc
     4801 cggcggatct atcgattagg aaaatgaagg gttaccagcc ggcgttggga tgaccgttaa
     4861 aatacggcac tccagaatgg cgataaccat atccaccgta atagggggta ccgccataat
     4921 aggcgtcgcc gccataatag ggccctccac cgtagtaggc cgggctgcta tcgtaataat
     4981 agtcgtctcg gcggttctgc gatgcggcga tcgcaagacc ggtgccgacg attccggcaa
     5041 aagcggctgc cgctgcggca ccaccgccgc cacggtaata gcgacgccgg gcgctgaagt
     5101 ccgtggcgcc gctggtcgct gccgccgccg atacgccctt gccggcggcc acagagcccg
     5161 cgagagccag cgatggctcg actgtggtca gcgccaccgc cgctacggtc gccatcacgg
     5221 cggcgcggcc aagggatgaa aacaaagcgt ttcgcgcgaa catctcaaaa tcctccatga
     5281 tggcccggcc tggcaggcct cccggagcca accagcgagc ccacaatagg ttccccgatc
     5341 ctgatccgag cggctggatc gtggcggtca tcgaccgttc agatatcatc cggcagaata
     5401 cccccgggtg ccccggggcg ccctgaaacc gatatagccg gcgcaaccgc tgtccggtcg
     5461 cgatgtagtc tcaatgcgcg taaatcaaag gaatgcggcc cgctgctttt tggcttcgct
     5521 gacgggcctg ctggttcttg ctattttcgg cctggcagcg agccgggcgg tgagcgacgt
     5581 tcggcaaccg gtcgcgatcc agtttaccct cgaccgaccg atcgatgcgg gtgctgcccc
     5641 gttcgtcatg gctgcggcgc gaggcatgtt cgcctcggaa ggtctcgcgg tcaccaccaa
     5701 tatcgccagc ggctcgcagg acgcgatcgc gcgcgtcgcc gccggcgcca gcgatttcgc
     5761 gcaggtcgac atcaacgcgc tgatccgctt tcgcgacaga ccgaaagctc cgcccatcaa
     5821 ggcggtattc gtgctgttca atcagtcgcc ctatgccatc atcgcccgca agaaccgcgg
     5881 catccacgcg ctctccgata tcgaaggcaa gaacctcggc gttggcgaag gcgacctttc
     5941 gatccggttg tggccggcgc tggcacggca aaacggaatc aagaccgcaa gcgtgaagca
     6001 aaacagcatc agcgccgccg tgcgcgaacc gatgctgtcg gcggggcagg tcgatgccgt
     6061 gaccgggttt tcatacctgt ccggggtcaa tttgagggat cgcggcgttc cggccgacga
     6121 tctggcggtg ctgcgcttcg ccgactacgg ctgcgaggcc tacgggtttt cggtgatcgt
     6181 caatccggcg ttcgcggccg ccaagcccga ggcggtgaga ggcttcctgc gtgcggtaat
     6241 tgccggcacc catctggcca tcaaggaccc cgcgcgcggg gctgatgagg ttgtcagccg
     6301 gatggacgac ggatcgcgtg atctcgagct ggaacgcctg gataccgtta tccgcgacaa
     6361 catcctcacc agcgaagtga agcgcgatgg catcggcggc atcgatccgg cccggctgga
     6421 gcggtcgatc gatcaaatcg ccgaggattt caaattccag aaacggccat ccgccaagga
     6481 tattttcgac gatgctttcc tgcctccgtt ggacggccgg ctgatcaatt gagcaaagaa
     6541 tcagctgaat ctccaggcga tccctgatta gaatgcgcgc ccgagcgatc aacggtcccc
     6601 gcgacccctt tgagtccccc gcatgtccct gctccgcctt tatacgcgcg tccttcattt
     6661 gctcggcaag gaggcacggc tgggctggat tctcgccggc gccaatctgc tgctggccgc
     6721 cgcgcagttc gccgagccgg tgctgttcgg ccgcatcgtc gatgttctct ccggcaaccc
     6781 gtccacgggt ccgctggcgt cgtcgtcagc gtggccgctg ctgggggcct gggcggcgtt
     6841 cggcctgttt accataggct gcagcgctgc ggtcgcactg catgccgacc ggctggcgca
     6901 ccggcagcgt caggcggtac tgacaaatta tttcgagcac atcatgcagc tgccgctgac
     6961 cttccacacc ggcacccatt ccggccgcct gatgaaggtg atgttgaacg gcaccgactc
     7021 gctgtggcgg ctgtggctgg catttttccg cgagcatttc gccgcgatca tgtcgctggt
     7081 ggtgctgctg ccgctggcgc tttacatcaa ctggcggctg gcgatcctgc tgttcgccct
     7141 gtgcgtggta ttcaccgtgc tgaccacgct cgtggtgcgc aagacctatg gcatgcaaag
     7201 cgaggtcgag gcgcattaca gcgatctgtc cgcccgcgct tcggatgcgc tcggcaatgt
     7261 cgcgctggtg caaagcttcg tgcgcatcga tgccgaggtg caagggttgc gcttcgtcgc
     7321 cgacaagcta ttggcggtgc agatgccggt gctgtcgtgg tgggcgctgg tcaccgtcat
     7381 cacccgggcg tcgaccacca tcaccgtgct ggcgatcttc accgtcggca tcgcgctgca
     7441 cgatcagggc atcacgtcgg tcggcgagat cgtgatgttc gtgagtttcg cgaccatgct
     7501 gatccagaag ctcgaacagg tggtcagctt catcaacaat gtgttcatgg aggcgccgcg
     7561 gctgcaggag ttcttccatg tgctggatgc cgtgcccgcg gtccgcgacc ggcccgacgc
     7621 gatcgacgcc ggacgactct cgggcctggt cgaattcaaa gacgtctcgt tttcgtatga
     7681 cggcaagcgg ccggcggtcg aagacctttc cttcaccgcg ctacccggcc agaccgtcgc
     7741 gctggtgggt ccgaccgggg ccggcaagtc gacggccatc gcgttgctgc atcgcgcgtt
     7801 cgatccgcag tcgggcttca tcaggatcga cggcatggat gtccgcggcc tgacgctgac
     7861 ggcgctgcgg cgaaatatcg gtgtggtgtt ccaggaggcc ttgctgttca accgttcgat
     7921 cgcggacaat ttgcgtgtcg gcaagccgga cgccaccgac caggaaatgc ggatcgccgc
     7981 cgcccgtgcc caggcgctcg aattcatcga gcgcagcgaa cggggcttcg agagcaatgc
     8041 cggcgagcgc gggcgcatgc tgtccggcgg cgagcgccag cggttgtcga tcgcccgcgc
     8101 gctgttgaag gatcccccga tcctgatcct cgatgaggcg accagcgcgc tcgatgcggt
     8161 caccgaagcc aagcttaacg ccgccctcga cgaggtgatg aagggacgca cgactttcgt
     8221 gatcgctcac cggctgtcga ccattcgcaa cgcgacacgg attctggtat tcgatagtgg
     8281 acgcgtcatc gagagcggaa ctttcgatga actggtcgcg aaaggcggtc gttttgcgga
     8341 actcgcaagg gctcaattca tggttcagga gccggcccgg gccagcattc atgcgaaggg
     8401 aaccgacagc gcggctctca agatttaggg attgccgaaa ttcagcccat ttcgtccacg
     8461 atttaatcgc acagcctctg ttctggaccg ataagccggt atagattcgt ccccgccaca
     8521 accggcggcg ccggacaggc ttgaaacccg aacatcacgc gccaaggcct cctcttcata
     8581 aggcctcctc ttcataagac ctcctcgttg aaggctggtc tcaaaggacc ggaatcggat
     8641 agtgcatctt cctcgcccgt tgccttgcaa gtcatcgtcg tgctggatcg ttcttctcgc
     8701 ggcgctcgct atcgttgctc cgcgcgtcgt gcaggcggag gcactgctgg tcgtcgaggc
     8761 cgataccggc aaggtcctgc aggccgacaa tgcgacctat ccctggtatc ccgcatcggt
     8821 gaccaagctg atgacggcct atgtcacgct caaggccgtc aaggatgggc acatcacgct
     8881 cgataccctg ttcacggtat cgcctgtcgc ggcctcgcag tcgccatcca agatgggttt
     8941 ccggccgggg acgcaggtca ccgtcgataa cgccctcaag atgatgctgg tgaaatcggc
     9001 caacgacatg gcggcggtgc tggctgaagg cgtcggcggt tcgatcgacg gtttttcggc
     9061 tcagatgaat caggccgcgc agcggctggg catgacgcag tcgagctacg tcaacccgaa
     9121 cgggctgccg gcggacggtc agatcacttc ggcgcgcgat ctggcgatcc tcgcccgtgc
     9181 gatcattcgc gacctgccgg aatacgagta tttcgtccac attccctcga tccgctacgg
     9241 ccgcaaggtc acgcagaatt tcaacaagct gatcgggcgc taccctggcg ccgatggctt
     9301 caagaccggc ttcatctgtg cttccggcta caatctggtg gcatcggcca cgcgcaacgg
     9361 caaacggctg atcgcggtgg tgctcggcgc gtcatccggc cagatgcgcg cggtgcgcgc
     9421 cgcccaactg ctcgagcgcg gttttgccaa taaccagctg gcgtggttgc ggccctcgct
     9481 cggcaccgtc gacaatctgg tgccgatcga cgcctcgccg cctaacctgc gcgacgagat
     9541 gtgcggggga aaacataaac gcccggccag cgacgaggat gaagacgccg tcgccagcaa
     9601 tgccagcggt ccaagcaccg gcgaaaccgc catcacgttt ttcaccgccg ggctgcagcc
     9661 gccgatggcg aggccctcgg acctgctggc ggcggaggcc gcgccctcgg agccgatccc
     9721 ggtctatacc ggtccgacca agaccgggcc tgccctgatc gccgccgtgg ccatggacag
     9781 cgaacagcag acgccaccgc accgcggcaa gaagaagtcg cgggtggcgt cgaaaaagcc
     9841 cgatactggg gccgaaccgg ccgccgcaaa acacgcgaat gccaaaccgg atggcacccc
     9901 aaaggtcgcg gaaaagccgg caaagcccaa ggccgtcgtc aagcccaagc ctgcgccgaa
     9961 gagcacgacc agccaggcca atccggtgga tcaaaaggcc gtcaccgcac cgcacacctg
    10021 agctggattt cggccgctcg gacgccccgg tcctggttag cgatgtggcg atagcgcgcg
    10081 gccgcttgcc atgggccgcc gcattgctca atactgccca aaacaaggcc tcccgtccca
    10141 aaacgggtta agcaaaccgg aaggggaaac accatggagc gcatctggct caagcaatat
    10201 ccggccggcg tgcccgccga tgtcgacgtg acccaataca catctctcgt cgagttgctg
    10261 gaagaaagct ttgcgaagtt cagcgaccgc aaggccttca tctgcatgga caggtcgatc
    10321 agctatcgcg atctcgacga gatgtcgctg gcgctcgcgg cctatctgca gagcaagggc
    10381 ctgcaaaagg gcgcccgcgt cgcgctgatg atgccgaacg tgctgcagta tccgatatcg
    10441 accgccgcag tgctgcgcgc cggctatacc gccgtgaacg tcaatccgct ctacaccccg
    10501 cgcgaacttg aacaccagct caaggattcc ggcgccgagg cgattatcgt tctggagaat
    10561 ttcgcgacca ccgtgcagca ggtgatggct agaaccgcgg tgaagcacgt catcgtcggc
    10621 agcatgggcg atctgctcgg cctgaagggc atgatcgtca atctggtggt acgccgcgtc
    10681 aagaaaatgg tgccggcgta ttcaattccg ggcgccgtcg ccttcaacga cgcggtcgtg
    10741 gccggccgca gcgtgaggct gaacaagccg aagctcgcgc ccgacgatgt cgccttcctg
    10801 caatacaccg gcggcaccac cggcgtctcc aaaggcgcga cgctgcttca tcgcaatatt
    10861 ctggccaacg tgctgcagaa cgatgcctgg ctgcagcccg cgctgaggaa gccgccgcat
    10921 gtcgaccaac tactcatcgt ctgcgcgctg ccgctctatc acatcttcgc gctgacggcc
    10981 tgtttcctgc tggcgatgcg cgccggaggc gtcaatctgt tgattcccaa cccgcgcgac
    11041 gtggccggct tcatcaagga gctgatgaaa taccaggtca acagtttccc cgcggtcaac
    11101 acgctctaca acgggctgtt gcattcgccc ggcttcgaca aggtcgactt ctccaagctg
    11161 aaaacctcgt tcggcggcgg catggcgacg cagaaggccg tcgcggaagc ctggctgaag
    11221 acaaccggct gtgcgctgtc cgaaggctat ggcctgtcgg aaacctcgcc gaccctgacc
    11281 tgcaaccccg ccgataccga caagttctcg ggctcgatcg gcctgcccgt tccttcgacc
    11341 tacctctcga tccgcgacga tgacggcaac gaggtgccgc tcggcgagcc cggcgaaatc
    11401 tgcgccaagg gcccgcaggt catggcgggc tactggaaca ggccggaaga gaccgcgaat
    11461 gtgatgaccg cggacggcta cttccgcacc ggcgacatcg gcgtgatgac gccggacggc
    11521 tacaccaaaa tcgtcgatcg caagaaggac atgattttgg tgtcgggctt caacgtctat
    11581 cccaacgaga tcgaggaggt catcgccagt caccccggcg tgctggagtg cgccgtgatc
    11641 ggcgtcgtcg acccgcggtc gggcgaagcc gtcaaggcgt ttgtcgtcaa gaaggacccc
    11701 aacgtcaccg ccgaagacat catcaagttc tgcggcacgc aattgaccaa ctacaaggtt
    11761 ccaaagcaga tcgagtttcg gaccagcctg cccaagacca atgtcggaaa gatcctgcgc
    11821 cgcgaattgc gcgatgaaaa gaaggccgcg gcctgaacga agatgatcgc gggcggctgt
    11881 tcatgaccgg cgcctgcgac ctcgcgccgg ccgatatcct cgcattctgg cgcgacgccg
    11941 gctgcgagcg ctggtacaag cgcgacgacg cgttcgacgc cgaggtccgc agccgcttct
    12001 tcgagctgtg gcagaaggcc gctgccggcg aactgtcatc ctgggaagca accgacgacg
    12061 gcgcgctggc gctggtcatc gtgcttgacc agtttccgcg caacatgttt cgcgacgatg
    12121 tcagggccta ttccagtgat gggctggccc gcgcggtcgc ggaccgcgcc attgcacgcg
    12181 gcgccgacat cggcgttgag gcaggcttgc tggagtttct ctacatgccc ttgatgcatt
    12241 cggagcaact ggccgaccag cagcggtgcg tcgaactgtt ccgcaaggcc ggcggcgcgg
    12301 acaatctgaa atacgccgag gatcacgccg agatcatccg ccggttcggg cgtttcccgc
    12361 atcgcaatcc tgttctcggg cgcccgacga cgccggcgga acaggccttt ctcgatagcg
    12421 acggattttc gggctgatga cagaacggtg aacgcccggg gccattgccg tttccgtggc
    12481 gccgaatatt gtgcctgcgg cctgcccggt ttaagacatt cccgcacagt caaggagaat
    12541 tgaagatgac catcaaagtc ggcgacaagc tgcccgaggc aaaatttcgc gtgatgacgg
    12601 cggaaggtcc gcaggtcaag accaccgacg atattttcaa gggcaagaag gtggcgctgt
    12661 tcgcggtgcc cggcgcctac accggcacct gccacaagat gcatctgccg agcattttt
//
LOCUS       DATNOI010000266         8135 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2228301,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000266 DATNOI010000000
VERSION     DATNOI010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2228301"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VN968_10375"
     CDS             complement(<1..394)
                     /locus_tag="VN968_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXL29679.1"
                     /translation="MPPQRFALPSEVPGLAARRIAADILDGVLHKHRTLDEQLDGAGA
                     HPGLKGLSDRDRALMRRLVATILRRLGTLGHVLSRLLDRGIPTDAPRAQSALLIGAAQ
                     ILWMDVPDHAAVDLSVRLVQSDRRAAKYA"
     gene            579..797
                     /locus_tag="VN968_10380"
     CDS             579..797
                     /locus_tag="VN968_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HXL29680.1"
                     /translation="MTDDASRPAAPAPAEPRKPLTPAAERALAEAKARSEAAEANVQP
                     AAKEFQGPKGPEPTRYGDWENKGIASDF"
     gene            complement(881..1393)
                     /locus_tag="VN968_10385"
     CDS             complement(881..1393)
                     /locus_tag="VN968_10385"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXL29681.1"
                     /translation="MSLRIALALAATIGAGMLMSSGAEARPDMVGFHGDYSPGTIVVK
                     TNERRLYLVLDDGHAVRYPVGVGKAGKQWAGTTRIDGKYLEPAWSPPAEVKHDKPSMP
                     DVIPGGSPRNPMGVAAMTLAGGEYAIHGTNMPGSVGGFVSYGCIRMLNDDIADLYQRV
                     SVGTTVVVSR"
     gene            complement(1816..3768)
                     /gene="acs"
                     /locus_tag="VN968_10390"
     CDS             complement(1816..3768)
                     /gene="acs"
                     /locus_tag="VN968_10390"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HXL29682.1"
                     /translation="MSEKIYGVPAEWAKRAWVDQAKYREMYARSVSDPNRFWAEQGKR
                     IGWIKPFTKVENASFAPGNISIKWFEDGVLNVAWNCIDRHLDKRGDQTAIIWEGDSPS
                     ESKHITYRQLHDEVCKFANILRTRNVKKGDRVTIYLPMLPEAAYAMLACARIGAIHSV
                     VFAGFSPDSLAQRITDCESKIVITADEGLRGGKKVPLKANVDAAIARSGGVDWVVVVK
                     RTGAAVDMNPSRDFWYHDAARMVTTECPVEHMHAEDPLFILYTSGSTGQPKGVLHTSG
                     GYLVFASMTHQYVFDYHDGDVFWCTADVGWVTGHSYILYGPLANGATTLMFEGVPNYP
                     DNSRFWNVVDKHKVNIFYTAPTAIRALMQGGDEPVKKTSRKSLRLLGSVGEPINPEAW
                     EWYHRVVGDERCPIVDTWWQTETGGILITPLPGATKLKPGSATEPFFGVQPEIVDADG
                     KVLGGECQGNLCIARSWPGMMRTVYGDHARFEQTYFSTYKGKYFTGDGCRRDADGYYW
                     ITGRVDDVINVSGHRMGTAEVESSLVAHAKVSEAAVVGYPHDIKGQGIYAYVTLMTGT
                     EPSEELRKELVAWVRKDIGPIASPDQIQFAPGLPKTRSGKIMRRILRKIAEDEPGSLG
                     DTSTLADPAVVDDLVAHRQNKKDAPA"
     gene            4179..5282
                     /locus_tag="VN968_10395"
     CDS             4179..5282
                     /locus_tag="VN968_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HXL29683.1"
                     /translation="MMDQQNFEAARPVSADPHVAMAPGHPASVESLARELGLKLGDQN
                     QLTIRRIKRGKSYSFVRANGTPIRHTGTIKRLHSMAVPPAYAEVRYAPDPTSHLQAVG
                     RDAAGRLQYRYHSDWEKVREHRKAHRLARLVAALPKIRRNVSAHLKGDAPTREFALSA
                     VIELIARTAIRPGNESYARLNGTRGATTLLKSNVVLEDDCVVLTFKAKGGKAVRKECD
                     AAKLVRAIGILRGVPGKRMFQYRDNSGNVRGVSTTQVNAFLREIAGIKISLKDFRTLM
                     ASAVVLESLSRISPAASARGRRKQVLEAVRGAADELSNTPAICRKSYVHDTIVTAFED
                     GILERFAATMKGYRSQSKREQLLAQVVSAAAAA"
     gene            complement(5524..5937)
                     /locus_tag="VN968_10400"
     CDS             complement(5524..5937)
                     /locus_tag="VN968_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HXL29684.1"
                     /translation="MNYWLVKSEPSSWSWDQQVAKGAKGEAWTGVRNFTARQNLVKMK
                     KGDRAFFYHSNEGKEIVGIAEIVKEAYPDPSDKTGKFVCVDIKADEPLKTPVTMAAIK
                     ADKRLTGMALVKYSRLSVQPVTAEEWKIVCKMAGM"
     gene            complement(6055..7035)
                     /locus_tag="VN968_10405"
     CDS             complement(6055..7035)
                     /locus_tag="VN968_10405"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HXL29685.1"
                     /translation="MPSFNSIAVIGAGAWGTALAGVAARAGRDVVLYARNAESAAQIA
                     ATRANPKLSGALLNTGVRVTDEIALAAAADVILIATPAQNLREAVTAIAPHLAKATPV
                     IACAKGIERGTHKFMTEVIAETAPDAHPAILSGPGFADDVARGLPTAVTLAATDEALA
                     ISLVQALGSSTFRPYHTTDVRGVEIGGAAKNVLAIAAGIVVGRKFGASAQAALTTRGF
                     SELVRLGRACGARSETMSGLSGLGDLILTCSSPQSRNFALGIALGRGERPPQDKLAEG
                     EFTAPVLVELAASQDVDMPVSSAVAAILSGATTIDEAIESLLTRPFKAEG"
     gene            complement(7052..8134)
                     /gene="tsaD"
                     /locus_tag="VN968_10410"
     CDS             complement(7052..8134)
                     /gene="tsaD"
                     /locus_tag="VN968_10410"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041801209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXL29686.1"
                     /translation="MTQSLVLGIETTCDETAAAVVERQSDGTGRILSNIVHSQTEEHA
                     AFGGVVPEIAARAHVDRLDGIVANAMKESGVGFAQLAAVAAAAGPGLIGGVIVGLTTA
                     KAIAMVHDTPLIAVNHLEAHALTPRLTGALDFPYCLFLASGGHTQIVAVVGVGNYVRL
                     GTTVDDAMGEAFDKVAKMLALPYPGGPEVERAAATGDPQRFAFPRPMLGRADANFSLS
                     GLKTAVRTEASQRTPLEPQDVSDLCAGFQAAVLESTADRLSVGLRLFHEQFGPPRALV
                     AAGGVAANQAIRGALQDVAEKAQTKLIIPPPALCTDNGAMIAWAGAERLAMGMIDTMD
                     APPRARWPLDEKAMAPAGFANTRAGF"
BASE COUNT         1474 a         2655 c         2527 g         1479 t
ORIGIN      
        1 cggcatattt cgcagcccgc cgatccgact gcaccagccg caccgacaga tcgaccgcgg
       61 catgatcggg cacatccatc cagagaattt gcgcggcgcc gatcagaagc gcgctctgcg
      121 cgcgcggcgc gtcggtcgga atgccgcgat cgagcagccg cgacagcaca tggcccaagg
      181 taccgagccg ccgcaagatc gtagcgacca gccgccgcat cagggcgcgg tcgcgatcgg
      241 agagtccctt taatcctgga tgggcgccgg cgccgtcgag ctgctcgtcg agcgtccggt
      301 gcttgtgcag cacaccatca agaatatccg ctgcgatccg ccgcgccgcg agaccgggca
      361 cttcggaggg cagggcgaat ctttgagggg gcatggagtg ggaacatctc gggggattga
      421 atggcgattc gcggtttgcg cgcatgtaat cgaaaaggtc acatgcgaat atgccaaata
      481 taagaatcgt ttttatcatt tcatagttcg agcgtgagct ttattatcaa agattgaatg
      541 gaacgagcgt ggttggtgcg gtggctggaa actctgaaat gacggacgat gcttcacggc
      601 ccgcggcgcc cgcgccggct gaaccgcgca agccgctcac gccggcagcc gagcgcgccc
      661 tcgccgaagc caaagcgcgc agtgaagccg ccgaggccaa cgttcagccg gcggcaaaag
      721 agttccaggg tcccaagggc ccggaaccca cgcggtacgg cgactgggag aacaagggca
      781 tcgcctcgga tttttaacct ccgcgtaagc ccatgatgga tatggttgcc gcaccaatag
      841 ccagtgcagc tggcgcgact tcaagtcctg atccggagga tcagcgcgag accaccaccg
      901 tcgtgccgac ggagacccgc tggtagaggt cggcgatatc gtcattcaac atgcggatgc
      961 agccatagga cacgaaaccg ccgaccgagc ccggcatgtt ggtgccgtgg atcgcgtatt
     1021 ctccgccagc cagcgtcatc gcggcgacgc ccatcggatt tcgcggcgag ccaccgggaa
     1081 tgacgtccgg catcgatggc ttgtcatgct tcacttcagc cggcggcgac catgccggtt
     1141 cgagatactt gccgtcgatc cgggtggtgc cggcccattg cttgccagcc ttgccgaccc
     1201 cgacgggata gcgcaccgcg tggccgtcat cgagcacgag atagagccgg cgctcattgg
     1261 tcttcaccac gatcgtcccc ggcgaatagt cgccgtggaa gccgaccatg tccggccgcg
     1321 cctcggcccc gctcgacatc aacatccccg ccccgatggt ggctgccagc gccagcgcaa
     1381 tcctcaacga catcgatctc tccactttcg cttcaagcac cccgttccgg gccgtcaaat
     1441 tccatgcaaa gcccgcgaat gccccggtgt gcagccggtt ttgccgacgc atttttaacc
     1501 gctcgcgtta accaaccggt tgccacgttg gccctgggac tcatttagtc gggggcaaga
     1561 aaggaaatgt tcaaaacaaa gtaaatgact tgaaaacaac actctttagg aaacgtgccg
     1621 cggacgggca gcttgccgct gacgatcgcg tgagtgtgcg gggggcaggg atcatgcgcg
     1681 ccatgtgtcc cggacgcgat gcagcgtctt cacgctgctc ggcaggagcc aggacccacg
     1741 aagtccagag aggaagtcca gagagtatgt gtgcgctgca tccggggaac ggcaccggcg
     1801 cgtggagcct tgctcctagg ccggcgcgtc cttcttgttc tggcggtgtg caaccagatc
     1861 gtcgaccacg gccggatccg ccagtgtcga ggtatcgccg agactgcccg gttcgtcctc
     1921 ggcaatcttg cgcaggatgc ggcgcatgat cttgcccgat cgggtcttgg gcaaccccgg
     1981 tgcgaactgg atctggtcgg gagacgcgat cgggccgatg tccttgcgca cccacgccac
     2041 cagttccttg cgcagctcct cgcttggttc ggtgccggtc atcagcgtca cataggcgta
     2101 gatgccctgg cccttgatgt cgtggggata gccgaccacg gcggcctccg agaccttcgc
     2161 atgcgccacc agcgagcttt cgacctcggc ggtgcccatc cggtggccgg agacgttgat
     2221 cacgtcatcg acgcgcccgg tgatccagta atagccatcg gcatctctgc ggcagccatc
     2281 cccggtaaaa tacttgccct tgtaggtcga gaagtaggtt tgctcgaagc gggcgtgatc
     2341 gccatagacc gtgcgcatca tccccggcca ggatctggcg atgcacagat tgccctggca
     2401 ttcgcccccc agaaccttgc cgtcggcatc gacgatctca ggctgcacgc cgaagaacgg
     2461 ctcggtcgcc gaacccggtt tgagtttggt ggcgccaggc agcggcgtga tcagaatgcc
     2521 gccggtctcg gtctgccacc aggtatcgac gatcgggcag cgctcgtcgc cgaccacgcg
     2581 atgataccat tcccacgctt ccggattgat cggttcgccg acgctgccga gcaaccgaag
     2641 actcttgcgc gaggttttct tgaccggctc gtcgccgccc tgcatcagcg cgcggatcgc
     2701 cgtcggcgcg gtgtagaaaa tgttgacctt gtgcttgtcg accacgttcc agaaccgcga
     2761 attgtcggga tagttcggca cgccttcgaa catcagcgtg gtcgcgccgt tcgccagcgg
     2821 cccatagagg atgtagctgt ggccggtgac ccagccgacg tcggcggtgc accagaagac
     2881 atcgccgtcg tgatagtcga acacgtattg atgcgtcatc gaggcgaata ccagatagcc
     2941 gcctgaggta tgcagcacgc ccttgggctg cccggtcgag cccgaagtat agaggatgaa
     3001 cagcgggtcc tcggcgtgca tatgctcgac cggacattcc gtcgtcacca tcctggcagc
     3061 gtcgtggtac cagaaatcgc gcgacggatt catgtcgacc gcagcgccgg tccgcttgac
     3121 cacgacgacc cagtcgacgc cgccgctcct ggcgatagcg gcatcgacat tggccttcag
     3181 cgggactttc ttgccgccgc gcagtccctc gtcggcggtg atgacgattt tggactcgca
     3241 gtcggtgatg cgctgggcga ggctgtcggg tgaaaagccg gcaaacacca ccgaatgaat
     3301 ggcgccgatc cgcgcgcagg ccagcatcgc gtaggcggct tccggaagca tcggaagata
     3361 gatcgtgacg cggtcgccct tcttgacgtt gcgggtgcgc aggatgttgg cgaatttgca
     3421 gacctcgtcg tgcagctgcc gataggtgat gtgcttcgac tccgaggggc tgtcgccctc
     3481 ccagatgatg gcggtctggt cgccgcgctt gtcgaggtgg cggtcgatgc aattccaggc
     3541 gacgttgagg acgccgtcct cgaaccattt gatcgaaatg ttgccgggag cgaaggacgc
     3601 gttctcgacc ttggtgaaag gcttgatcca gccgatgcgc ttgccctgtt cggcccaaaa
     3661 gcgattggga tcgctgaccg aacgtgcata catctcgcgg tatttggcct ggtcgaccca
     3721 tgcgcgcttg gcccattccg ccggcacacc gtaaattttc tcggacatct taaccctcca
     3781 cccatgcggc cagtgaacgc cgacactgca agccgtcttc attgttataa cgattatgcg
     3841 tcgggcctga tggcgccgac aagcggaaca aatagtacct ttggcggtgc tcctcgccat
     3901 gcgcaggatc aaaccgtccg acgacgcggc aggggtagcc ccggcatccg ttcagcatcc
     3961 aacgccatca cgatcgcgtt ttccgataac caaaaaatcc ccgaacaggc cagctttgct
     4021 gccaagaggt atctaaagac cgtcgccttc cacttcttgg gctggcgctc gcggcaatta
     4081 ccccctaaat ggcgtcccct aggtcgtgcg gtctgccgga accaaagatc aagaaccggc
     4141 attaccgcgc gcgcatcagg agcaaggcat ctaaggtcat gatggatcag cagaatttcg
     4201 aggctgcgcg gcccgtttcc gccgatccgc atgtggctat ggcgccggga cacccggcaa
     4261 gtgtcgaatc actcgcccgg gaactgggtt tgaagctcgg cgaccagaac caattgacca
     4321 tccggcgcat caagcgcggc aagagctatt cgttcgttcg cgccaacgga acgccgatcc
     4381 gccacaccgg caccatcaag cgcctccact cgatggcggt gccgccggcc tatgccgaag
     4441 tgcgctacgc ccccgacccg acctcgcacc tgcaggccgt cggccgcgat gccgccggcc
     4501 ggttgcaata ccgctatcac tccgattggg aaaaggtccg cgagcaccgc aaggcgcacc
     4561 ggctggcgcg gctggtcgcg gccttgccga aaattcgccg taacgtctcg gcgcatctca
     4621 agggcgacgc gccgacccgc gaattcgcgc tgtcggcggt gatcgagctg atcgcgcgca
     4681 ccgcgatccg tcccggcaac gaatcctacg cccgcctcaa cggcacccgc ggcgccacca
     4741 cgctgctgaa gtccaatgtc gtgctggaag acgactgcgt cgtgctgacc ttcaaggcca
     4801 agggcggcaa ggccgtgcgc aaggaatgcg acgccgccaa gctggtgcgc gccatcggca
     4861 tccttcgcgg cgtgccgggc aagcggatgt tccagtaccg cgacaattcc ggcaatgtcc
     4921 gcggggtctc gaccacacag gtcaacgcgt tcctgcgcga gatcgccggc atcaagatat
     4981 cgctgaagga ttttcgtacc ctgatggcgt ccgccgtggt gctggagtcg ctgtcgcgga
     5041 tttcgccggc cgccagcgcc cgcggccgtc gcaaacaggt gctggaggcc gttcgcggcg
     5101 ccgccgatga actttccaac accccggcga tctgccgcaa gagctacgtc cacgacacca
     5161 tcgtcaccgc attcgaagac ggcatcctcg agcgttttgc cgcaacgatg aaaggctatc
     5221 gctcgcagtc gaaacgcgag cagcttttgg cgcaagtggt gtcagccgcc gcggcggcgt
     5281 agctttcttc gtcttccttc tccccttgtg ggagcaggtg gccataggcg gccttcggcc
     5341 gccgtccttt aagaggacgc cgatgcttta catcggctgc gcgaagttcg tgccggatga
     5401 ggggttacgc cctatcgata gaccgtaacc cctcacccgt cttcgcttcg cgaagccacc
     5461 ctctcccacc aggggaccca caaggggaga ggggaagtaa gcagctgagg aaagcgccgg
     5521 gttttacatt cccgccatct tgcagacgat cttccactct tccgccgtca ccggctgcac
     5581 cgacagcctt gaatatttca ccagcgccat gccggtcagc cgcttgtcgg ccttgatcgc
     5641 ggccatcgtc accggcgtct tcaggggttc gtcggctttg atgtcgacgc agacgaattt
     5701 gccggtcttg tcgctgggat cgggataggc ctccttgacg atctcggcga tgccgacgat
     5761 ctccttgccc tcgttggaat gatagaagaa ggcgcggtcg cccttcttca tcttcacgag
     5821 gttctgtcgg gctgtgaaat tgcgcacgcc ggtccaggct tcgcccttgg cgccctttgc
     5881 cacctgctga tcccacgacc agctcgaagg ttccgatttc accagccagt agttcatgcc
     5941 gcccaccatc tacccgtcat ggccggactt gttccggcca tccatgtcct tgcttattgc
     6001 cgttttatca agacgtggat gcccgcgaca agcgcgggca tgaggaagcg ggaactatcc
     6061 ctccgccttg aacgggcgtg tcagcagact ttcgatcgct tcgtcgatcg tcgttgcacc
     6121 gctcaggatt gccgcgaccg cacttgatac cggcatgtcg acatcctgcg aagccgccag
     6181 ttcgaccagc acgggtgcgg tgaattcgcc ttcggccaac ttgtcctgtg gcggccgttc
     6241 gccacgcccg agcgcgatgc cgagcgcaaa attgcgcgac tgcgggctgg agcaggtcag
     6301 gatcagatcg cccaggcccg aaagtcccga catcgtttcg ctgcgcgcgc cgcaggcgcg
     6361 cccgagccgc accaattcgc tgaagccgcg cgtggtcaac gcggcttgcg ccgacgcgcc
     6421 gaatttcctg ccgaccacga ttccggcggc gatcgccagc acgttttttg cggcgccgcc
     6481 gatctcgacg ccgcggacat ctgtcgtgtg atagggccgg aaggtcgacg agcccagcgc
     6541 ctgcaccagg gagattgcga gcgcctcatc ggtcgccgcc agcgtgaccg ccgtaggaag
     6601 gccgcgcgcg acgtcgtcgg caaaacccgg tcccgacaga atagccgggt gcgcatcggg
     6661 ggctgtttcc gcgatcacct cggtcatgaa tttgtgggtg ccgcgctcga tgcccttggc
     6721 gcaggcgatc acgggtgttg ccttggcgag atggggcgcg atcgccgtca cggcttcgcg
     6781 caaattctgc gcgggcgtgg cgatcaggat gacgtccgct gccgcggcca gcgcgatctc
     6841 gtccgtcacg cggacgcctg tgtttagcag cgcgccggac aatttcggat tggcgcgcgt
     6901 tgccgcaatc tgcgctgcac tctccgcgtt gcgcgcataa agaacgacat cgcgcccggc
     6961 gcgcgcggcg acgccggcca gcgcggttcc ccatgcgccg gcgccgatca ccgcgataga
     7021 gttaaaggac ggcatcgcgg tcgatctccc gctaaaaacc cgcgcgcgta ttggcgaatc
     7081 ccgccggcgc catcgccttt tcatcgagcg gccagcgcgc gcggggcggg gcatccatcg
     7141 tatcgatcat ccccatcgcc agccgttccg cgccggccca ggcaatcatg gcgccattgt
     7201 cggtgcacag cgcgggcggc ggaatgatca gcttggtctg cgctttctcg gccacatcct
     7261 gcaacgcgcc gcggatcgcc tgattggcgg caacgccgcc ggcggcgacc agcgcgcgcg
     7321 gcgggccgaa ttgttcgtga aacagcctca agccgacact gagacgatcc gccgtcgatt
     7381 ccagcaccgc ggcctggaaa ccggcgcaga ggtcgctgac gtcctgcggt tccagcggcg
     7441 tccgctgact ggcctcggta cgcaccgcgg tcttcaatcc cgacaacgag aaattggcgt
     7501 cggcgcgccc cagcatcggc cgcggaaagg caaaccgctg cggatcgccg gtggccgcgg
     7561 cgcgctcgac ctccggcccg cccggatagg gcagcgccag catttttgcc accttatcga
     7621 aagcttcgcc catggcgtcg tcgacggtgg tgccgagacg gacatagttc ccaacgccga
     7681 caacggcgac gatctgggtg tggccgccgg aggcgagaaa aaggcaatac ggaaagtcga
     7741 gcgcgccggt cagccgcggc gtcagcgcgt gggcctcgag atggttcacc gcgatcagcg
     7801 gcgtatcatg caccatcgcg atggcctttg cggtggtcag gccgacgatc acgccgccga
     7861 tcagcccggg gccggcggcg gcggcgactg ccgcgagctg ggcaaagcca actccggatt
     7921 ccttcatcgc attggcgacg atgccgtcga gccgatcgac atgggcgcgc gccgcgatct
     7981 ccggcactac gccaccaaaa gcggcatgct cttcggtttg ggagtggaca atattggaca
     8041 aaatccgtcc ggtcccgtcg ctttggcgct cgaccacggc ggcggcggtt tcatcgcagg
     8101 tggtctcgat cccgagaacc agactttgcg tcagc
//
LOCUS       DATNOI010000267         6142 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2332079,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000267 DATNOI010000000
VERSION     DATNOI010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2332079"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..253
                     /locus_tag="VN968_10415"
     CDS             26..253
                     /locus_tag="VN968_10415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HXL29687.1"
                     /translation="MDPIAAKYIGAGIACIGMGGAGAGVGIIFGNYLAAAVRNPSAAQ
                     GQFGNLIFGFAVTEALGIFSLLIALLLLFAL"
     gene            319..876
                     /locus_tag="VN968_10420"
     CDS             319..876
                     /locus_tag="VN968_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HXL29688.1"
                     /translation="MAESHGDAKGATAHTEAEGGHKAPFPPFQKDTFASQLVSLLIAF
                     VALYLIVSRIALPRVGSLLDERQKKIEGDLAAAQTLRDESDAALKAYESELAAARSRA
                     QAIGTETREKLNATSEAERKTLEERLSVKLAEAEKTIASTREAAMTNVRGIASDAAIA
                     IVQRLSGLAPDRRTVNKAVDASLKG"
     gene            882..1367
                     /locus_tag="VN968_10425"
     CDS             882..1367
                     /locus_tag="VN968_10425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195257.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HXL29689.1"
                     /translation="MFAEPEFWVAVAFVILMGVFAYFGIHRTVLKTLDHRSDRIKAEL
                     DDARRLKDEAAKVLAEYESRHASAEREAQDIIASARAEAERIAAEAKTKMEDFVTRRT
                     KTAESKIALAEAQALADVRAAAANAAVAAASAILSKSVKGEVADDLLAKGIAEVREKL
                     N"
     gene            complement(1551..2201)
                     /locus_tag="VN968_10430"
     CDS             complement(1551..2201)
                     /locus_tag="VN968_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29690.1"
                     /translation="MAAGRAGRLRVALAFAVAGALSGCGGGSMFGASSDSSPSISDRF
                     GQLFGSKSQAVGEAPPPAVDNELTCPSVSIRAGASTYAVAAPGKQAVGNDLRYQATIT
                     RTARDCNLNGDQITARIGIQGRVIAGPAGNPPTIEIPIRVAVVQSGVQEKTIATKVYR
                     TTVSMEEGSSVPFSLVAEDLVYPVPPGATGDSYVFYIGFDPQALKPEPKAATRRKK"
     gene            complement(2361..2948)
                     /locus_tag="VN968_10435"
     CDS             complement(2361..2948)
                     /locus_tag="VN968_10435"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HXL29691.1"
                     /translation="MALFRLPSSGPAALAPRGNGLLLRAPQMSDFLQWAHLREYSREY
                     LTPWEPIWPSDDLTRSGFRRRLRRYAEDVAADRSYPFIVFRESDGAMIGGVTLANVRR
                     GIVQAGTIGYWVGQPHAHRGYMTSALRVLLPSLFGELNLHRVEAACIPSNAPSIRVLE
                     KCGFNREGLARRYLCINGVWQDHLLFGLLHEDFRG"
     gene            complement(3070..4359)
                     /locus_tag="VN968_10440"
     CDS             complement(3070..4359)
                     /locus_tag="VN968_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXL29692.1"
                     /translation="MSVDVTKLPSGLTIITDTMPHLETAALGVWAGVGGRDEKPNEHG
                     ISHLLEHMAFKGTARRSSREIVEEIEAVGGDLNAGTSTETTAYYARVMKADVPLALDV
                     LSDILANPSFVPDELEREKSVIVQEIGAAQDTPDDVVFEHLNELCYPDQPMGRSLLGT
                     AKTLKGFNRDTLRGYLSTHYRGPDMVVAAAGAVDHKRVVEEVTQRFSSFDGVAAPKPQ
                     AATFGKGGSRVVHRELEQAHLTLALEGVPQTDLSLFSLQVFTNTLGGGMSSRLFQEVR
                     EKRGLCYSIYAFHAPYSDTGFFGLYTGTDPGDAPEMMEVIVDVMNDAVETLTEAEVAR
                     AKAQMKAGLLMALESCSSRAEQLARHVLAYGRPLTVEELVTRIDAVSVESTRNAARAL
                     LSRSRPAVVALGSGRGLDTAVAFAEGLTRSKAKTLLH"
     gene            complement(4356..5774)
                     /gene="thrC"
                     /locus_tag="VN968_10445"
     CDS             complement(4356..5774)
                     /gene="thrC"
                     /locus_tag="VN968_10445"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXL29693.1"
                     /translation="MTRYISTRGEAPALGFCDVMLTGLARDGGLYVPEAWPQLSLDTI
                     AGFFGRPYWEVAVDVIRPFVGGEIPDAELGRMANEAYATFRHPAVVPLSQVSPNQFVL
                     ELFHGPTLAFKDVAMQLISRLMDHVLAQRAQRTTIVVATSGDTGGAAVDAFAGIENVD
                     LVVLFPHGRISDVQRRMMTTTGAANVHALAVEGTFDDCQAIVKGLFNHHGFRDQVSLS
                     GVNSINWARIVAQVVYYFTSAVALGSPARTVDFTVPTGNFGDIFAGYVAKRMGLPIRW
                     LRIAANVNDILPRTLKTGIYEVREVHASASPSMDIQVSSNFERLLFEASRRDAASVRR
                     LMASLRLSGRFVLPDATLAAIREEFDAGRADETETAAAIRAAWREAGDLVDPHTAVAL
                     AVADRDTPDLRIPNIVLSTAHAAKFPDAVEAACGVRPQLPPWLEGLMSRPEHIRVMNN
                     DQVEVERFVLSVSRAAKQGVAG"
     gene            complement(5910..>6142)
                     /locus_tag="VN968_10450"
     CDS             complement(5910..>6142)
                     /locus_tag="VN968_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HXL29694.1"
                     /translation="AMARALGWGGNGLKVAPFYIDLEQPLPASGIPKPGPLEVHLKDD
                     HLQYAITWFGLAGAVVIAFGVWWQGQRRLASS"
BASE COUNT         1183 a         2044 c         1879 g         1036 t
ORIGIN      
        1 caaacctcaa gaaggagttc gtttcatgga tccgattgcc gcgaagtaca ttggcgctgg
       61 tattgcctgc attggcatgg gtggcgccgg cgcaggcgtg ggcatcatct tcggcaacta
      121 tctcgccgcc gcggtgcgca atccgtcagc tgcccagggc cagttcggca acctgatttt
      181 cggcttcgcg gtgaccgaag cgctcggcat cttctcgctg ctgatcgcgc tgctgctctt
      241 gttcgcgctc tgatccatca ccgatctggc caatgagatt ttcggttcga atctgcattg
      301 accgaacagg agaagcccgt ggctgagagt catggcgacg ccaagggcgc gaccgcccac
      361 accgaggcag agggcggcca caaggccccc ttcccgccgt tccagaagga cacctttgcg
      421 tcgcaactgg tgtcgctgct gatcgcattc gtcgcgcttt atctgatcgt ttcgcgaatt
      481 gcgctgccgc gcgtcggcag cctgctcgac gaacgccaga aaaagatcga gggcgatctg
      541 gccgcggcgc aaacgctgag ggacgagtcc gacgcggcgc tgaaagccta tgagagcgag
      601 ctcgccgccg cgcgctcgcg cgcgcaggcg atcggcaccg agacccgcga aaagctcaac
      661 gcgacctccg aggccgagcg caagacgctg gaagagcggc tgtcggtcaa actcgccgaa
      721 gccgaaaaaa ccattgcgtc gacccgcgaa gcggcgatga ccaacgtccg cggcattgcc
      781 tcggacgcgg ccatcgcgat cgtgcagcgg ctctccggcc tggctcccga ccgcaggacc
      841 gtcaacaagg cggtcgacgc ctccttgaag ggataatcct gatgttcgcc gaaccggaat
      901 tctgggtcgc cgtcgccttc gtcatcctga tgggggtgtt cgcctacttc ggcatccacc
      961 gcaccgtgct caagacgctg gaccatcgca gcgaccgcat caaggccgaa ctcgacgacg
     1021 cccgccgcct caaggacgag gcggctaaag tgctcgccga atacgagtcc cgccacgcca
     1081 gcgcggaacg cgaggcgcag gacatcatcg catccgcaag ggccgaagcc gagcgcatcg
     1141 cggccgaagc caagaccaag atggaagatt ttgtcacccg gcgcaccaag accgccgaaa
     1201 gcaagatcgc gctcgccgaa gcccaggcgc tggccgatgt gcgtgcggcc gcggccaacg
     1261 ccgcggtggc cgcggcttcc gccatcctgt cgaaatcggt gaagggcgag gtcgccgacg
     1321 atctgttggc caagggcatc gccgaagtcc gcgagaagct gaactgacag attcaattcg
     1381 taacagcaga agccggtgca atcagcgccg gctttttcgt tgtgtcatcg ctcgcgctta
     1441 ttgatgtcat cacccgcgaa agcgggtgat ccagattcca gagcgttaga attaatcacc
     1501 gccgtcacgg aatactggat cgcccggtca agccggcgat gacaacaaga ttacttcttc
     1561 ctgcgcgtcg cggccttcgg ctccggcttc agcgcctgcg gatcgaatcc gatatagaag
     1621 acgtaggaat cgccggtcgc tcctggcggc accggataca ccagatcctc ggccaccaga
     1681 ctgaacggca cgctgctgcc ttcctccatc gacacggtgg tccggtagac cttggtggcg
     1741 atcgtcttct cctgcacgcc ggactgcacc accgcgacac gtatcggaat ctcgatcgtc
     1801 ggcggatttc ctgctgggcc ggcaatgacg cggccctgaa tgccgattcg tgcggtgatc
     1861 tggtcgccgt tcagattaca atcgcgcgcg gtgcgggtga tcgtcgcctg ataacgcaga
     1921 tcgttgccga cggcctgctt gccgggtgcc gccaccgcat aggtggacgc gccggcgcga
     1981 atgctcaccg aggggcaggt caattcattg tctaccgccg gtggcggcgc ctcgccgacg
     2041 gcttgcgatt tcgagccgaa cagctggccg aacctgtcgc tgatcgacgg gctggaatcg
     2101 gacgaggccc cgaacatgct accgccgccg cacccggaca aggcgcccgc aacggcgaac
     2161 gccagcgcga cgcgcagacg gcccgcccga ccggcagcca tcgtccgcat catcagcttg
     2221 ttacgcttca tccggttatc ctctgggacg tatcgacgca gtttacgcgt gaaaaggtgc
     2281 gcgcttttgc gcgttataac agcgcaatgc cggcgaaccc aaggcgccaa aaaagcccgt
     2341 gccacaatct aacaacaagg ttagccgcga aaatcctcat gcagcaggcc aaacaaaagg
     2401 tggtcctgcc agacaccatt gatgcagaga tagcgtcgcg ccaggccttc gcggttaaag
     2461 ccgcatttct ccaaaacccg gatcgagggc gcattggagg gaatgcaggc ggcctcgacg
     2521 cgatgaagat tgagttcccc gaacagcgac ggcaatagca cgcgcagcgc cgatgtcatg
     2581 tagccgcgat gcgcgtgcgg ttgcccgacc cagtagccga tggtaccggc ctgcacgatg
     2641 ccgcggcgga cgttggcgag cgttaccccg ccgatcatcg ccccatccga ttcgcgaaat
     2701 acgatgaacg gataggacct gtcggcggcg acgtcttcgg catagcggcg caaccgtcgc
     2761 cgaaagcccg agcgcgtcag atcgtcggac ggccagatcg gctcccacgg cgtgaggtat
     2821 tcccggctat attcgcgcaa atgcgcccat tgcagaaaat cggacatctg cggcgcgcgc
     2881 agcagcaggc cgttgccgcg cggcgcaagc gccgccggtc cgctggatgg caaacgaaac
     2941 agggccatgt ctgtactccc ctgcccaact ctgggcgtcc caaagcaccc tattgtctga
     3001 gcatgatctc ttcggaaaac cggtgtcgac cctcggatca ggcccgaggg catgcttccg
     3061 gatcatgctc tagtgaagca gcgtcttggc cttcgatcgg gttaatcctt ccgcaaaagc
     3121 caccgccgtg tccagacccc tgccgctccc aagcgcgacc accgccggac gactgcgcga
     3181 caacagggcg cgcgccgcgt tgcgcgttga ttccacgctg acggcgtcga tcctggtcac
     3241 cagttcttcc accgtcagcg ggcgcccata ggccagaaca tgccgcgcca gctgctcggc
     3301 gcgcgaactg cagctttcca gcgccatcaa tagccccgcc ttcatctgcg ccttggcgcg
     3361 cgcgacctcg gcttccgtca gggtctcgac ggcgtcgttc atgacatcga cgatcacctc
     3421 catcatctca ggcgcgtcgc ccggatcggt gccggtatag agcccgaaaa acccggtgtc
     3481 ggaatacggc gcatgaaaag cgtagatcga atagcacagc ccgcgtttct cgcgcacctc
     3541 ctggaacagc cgcgacgaca tcccgccgcc caacgtattg gtaaaaacct gcaaggagaa
     3601 caacgacagg tcggtctgcg gcaccccctc gagcgccagc gtcagatgcg cctgttcgag
     3661 ttcgcggtgc accacgcggg agccgccctt gccgaacgtc gcggcttgcg gctttggcgc
     3721 ggccacgccg tcgaagcttg agaaccgctg cgtcacttcc tcaaccaccc gcttgtgatc
     3781 gacggcgccg gccgccgcca ccaccatgtc cgggccgcgg taatgcgtcg aaaggtagcc
     3841 gcgcagcgta tcgcggttaa aacccttcaa ggtctttgca gtgccgagca atgagcgccc
     3901 catcggctga tccgggtaac acaattcgtt gagatgttcg aacaccacgt cgtcgggcgt
     3961 atcctgcgcg gcaccgatct cctgcacgat gacgctcttt tcgcgctcca gctcgtcggg
     4021 cacgaacgat ggattggcga gaatgtcgga cagcacgtcg agcgccagcg gcacgtcggc
     4081 tttcattacc cgcgcataat aggccgtggt ttcggtcgag gtgccggcgt tgagatcgcc
     4141 gccgaccgcc tcgatttcct cgacgatctc gcgcgacgag cgtcgcgcgg tgcccttgaa
     4201 cgccatgtgt tcgagcagat gcgagatgcc gtgctcgttc ggcttttcgt cgcggccgcc
     4261 gacgccggcc cacaccccga gcgccgcggt ctcgagatgc ggcatggtat cggtgatgat
     4321 ggtaaggccg gagggaagct tggtgacatc gacgctcatc cggcaactcc ttgcttcgcg
     4381 gcgcggctga ccgacagcac gaaccgctcg acttcaacct gatcgttgtt catgaccctg
     4441 atgtgctcgg gcctggacat cagaccctcg agccagggcg gcagctgcgg acgcacgccg
     4501 caggcggcct ccaccgcatc gggaaatttc gccgcatgcg cggtcgacag cacgatgttg
     4561 ggaatcctca aatccggagt gtcgcgatcg gctaccgcca gcgccaccgc ggtatgcgga
     4621 tcgacaagat cgccggcctc gcgccaggcg gcgcggatgg cggctgcggt ctcggtctcg
     4681 tcggcacgcc cggcgtcgaa ttcctcgcgg atcgccgcca gtgtcgcgtc gggcagcacg
     4741 aaacgcccgg acagtctcag cgacgccatc agccggcgca cgctggccgc gtcgcggcgg
     4801 ctcgcctcga acagcagccg ctcgaaattc gaggagacct gaatgtccat cgacggcgac
     4861 gccgacgcgt gaacctcgcg cacttcatag atgccggtct tgagcgtgcg cggcaggata
     4921 tcgttgacat tggcggcgat ccgcaaccac cggatcggca gacccatgcg cttggcgaca
     4981 tagccggcaa agatgtcgcc gaaattcccg gtcggcacgg tgaaatccac cgttcgcgcg
     5041 ggcgagccga gcgcgaccgc ggacgtgaaa taatacacca cctgggcgac gatccgcgcc
     5101 cagttgatgg aattgacacc cgatagcgaa acctgatcgc gaaagccgtg gtgattgaac
     5161 agccccttca cgatcgcctg gcaatcgtca aacgtgcctt cgaccgcgag cgcgtgaacg
     5221 ttggcggcac cggtcgtggt catcatccgc cgctgcacgt cggagattcg gccgtgcgga
     5281 aacagcacga ccagatcgac attttcgatt cccgcaaacg catcgaccgc ggcgccaccg
     5341 gtatcgccgg aggtcgcgac cacgatggtg gtgcgctgcg cccgttgcgc cagcacgtga
     5401 tccatcagcc gcgagatcag ctgcatcgcc acgtccttga acgccagcgt cggcccgtgg
     5461 aacagctcca gcacgaattg attggggctg acctggctga gcggcaccac cgcaggatgg
     5521 cggaacgtgg cataggcctc gttggccatg cggccgagtt cggcatcggg aatctcgccg
     5581 ccgacaaacg ggcggatcac gtcgaccgcg acttcccaat acggccggcc gaaaaagccc
     5641 gcgatggtat caagcgaaag ctgtggccag gcttcgggca cgtagaggcc gccgtcgcgg
     5701 gcgagcccgg tcagcatcac gtcgcagaat cccagggccg gggcctcccc ccgcgtcgaa
     5761 atataccgcg tcaaaccgcc ctccaaaggc ctaggcgccc gccaaacctt tgatattaat
     5821 gggttatata actttgccgg cgacaaagcc cgcgacacca taaagcgttt tcgacggaag
     5881 gggaaatcgg gattctgccc tcctgtcgct caagacgagg cgaggcgcct ctgcccttgc
     5941 caccacactc cgaacgcgat caccaccgcg cccgccaagc cgaaccaggt gatggcgtat
     6001 tgcaggtgat cgtccttgag atggacctcg agcgggccgg gttttgggat gccgcttgcg
     6061 ggcagcggct gctccagatc gatgtagaac ggcgcgacct ttaggccatt cccgccccag
     6121 ccgagcgccc gcgccatcgc cg
//
LOCUS       DATNOI010000268         5123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1999631,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000268 DATNOI010000000
VERSION     DATNOI010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999631"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /gene="aroC"
                     /locus_tag="VN968_10455"
     CDS             complement(<1..800)
                     /gene="aroC"
                     /locus_tag="VN968_10455"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXL29695.1"
                     /translation="MSFNTFGHMFRVTTFGESHGVAIGCVVDGCPPLLPLAVEDIQHD
                     LDRRRPGQSRFTTQRQEADAVKILSGVMAHPETGAQVTTGTPIALLIENTDQRSKDYS
                     DIKDKFRPGHADYTYEAKYGLRDYRGGGRSSARETATRVAAGAIARKILPGVTVRGAL
                     VQMGPHQIDRDKWDWDEIARNPFFCPDKDKAAFFESYLDDIRKKGSSIGAVIEVVAEG
                     VPAGLGAPIYAKLDADLAAALMSINAVKGVEIGSGFGAAELSGEENAD"
     gene            complement(1036..1635)
                     /locus_tag="VN968_10460"
     CDS             complement(1036..1635)
                     /locus_tag="VN968_10460"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXL29696.1"
                     /translation="MPAPTIYYIRHGETEWNAAGRFQGSQDIPLNDLGRTQAVTSGGI
                     LAGVLSRDGRDPSSLPFVASPLGRARSTMELVRGAIKLPPGDYAIDDRLREIGYGQWE
                     GLTLPEMQQQDAATFASRNEDKWGVAAPSGESYASVTLRVREWFDSLVADTVAVAHGG
                     TMRALMVALGIANPLEAAETPIGQGVVYVFSDGGMEKYG"
     gene            complement(1647..2477)
                     /gene="fabI"
                     /locus_tag="VN968_10465"
     CDS             complement(1647..2477)
                     /gene="fabI"
                     /locus_tag="VN968_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HXL29697.1"
                     /translation="MSQSSGLMQGKRGVILGVANSRSIAWGIAKACHAAGAEIAFTWQ
                     GDALKKRVEPLAKELGGILLGHCDVTDPATIDAVFNVLREKWGKIDFVVHAIAFSDKD
                     QLDGRYLETTADNFSKTMLISCYSLTAIAQRAEKLLTDGGSILTLTYYGAEKWMPHYN
                     VMGVAKAALEASVRYLAADLGEKNIRVNAISAGPIKTLAASGIGDFRYILKWNEYNAP
                     MRRTVTTEEVGDSAMYLLSDLSRGVTGEVHHVDSGYHVVGMKRPDAPDISLSNSLSKE
                     "
     gene            2675..3163
                     /locus_tag="VN968_10470"
     CDS             2675..3163
                     /locus_tag="VN968_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXL29698.1"
                     /translation="MTAFRQSIEAMIPALRRYARALARDADIADDLVQDTLVRALRSE
                     RLFLGGDVRSWLYTILTNLNKNRRRSLARRPQFMPLLDNNPDASGTEAEGRDIARALA
                     TLVEEQRAVLLLVMLEGLSYREVADIQGVPIGTVMSRLARARAHVKASLEGERVALRR
                     VK"
     gene            3474..4244
                     /locus_tag="VN968_10475"
     CDS             3474..4244
                     /locus_tag="VN968_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HXL29699.1"
                     /translation="MTDPNISITEDELHAYVDKELPAERRGDVEAWLAAHPDDAERVQ
                     SWRAMAEALHARYDSVADEAVPKRLEIERLERQPRKWLYGAIAATLLAFVGGGGVGWL
                     ARGAAASPSAFQKLTVDALDAYRLYVVEVRHPVEVPGSERTHLQQWLTKRCGWDVHAP
                     ELDATGLKLVGGRLLPGPTGPASFLMYESASGERFTLYTSRAKTGTAQMRYTAAENSG
                     AMYWSEDGVGYVLSGPIDKDRLNQVARLVYDQTEKTPG"
     gene            complement(4489..>5123)
                     /locus_tag="VN968_10480"
     CDS             complement(4489..>5123)
                     /locus_tag="VN968_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HXL29700.1"
                     /translation="GGFEDILNSMFGGAAARGARPGAGSSFEFDPGGIAIDLDLNVTM
                     TVSLEEAVHGVTKRVRLPTGKELDVKIPAGVVAGQQIRLKGQGETAPGHRPGDLLITV
                     SIAPHAIFKVDGSDLRVDLPITLYEAVLGGKVRVPTLGSAVELSIPKNTSSGRTFRLK
                     GKGLPKATGMTGDLFVTTRIMLPDGNDAELEALMQKWRDGHPYNPRSDMT"
BASE COUNT          907 a         1640 c         1628 g          948 t
ORIGIN      
        1 tcgtcggcgt tttcctcgcc ggacaattcg gccgcgccaa accctgagcc gatctcgacg
       61 cccttcaccg cgttgatgct catcagcgcc gcggcgaggt cggcatcgag tttggcgtag
      121 atcggcgcgc cgaggccggc cggcacgcct tcggcgacga cctcgatcac cgcgccgatc
      181 gacgatccct tctttcggat gtcgtcgaga tagctctcga agaacgcggc cttgtccttg
      241 tccgggcaga agaacggatt cctggcgatc tcgtcccaat cccatttgtc gcgatcgatc
      301 tggtgcgggc ccatctgcac cagcgcgccg cgcacggtca cgccgggcag gatcttgcgg
      361 gcgatggcgc cggccgcgac ccgcgtcgcg gtctcgcgcg ccgacgaacg cccgccgccg
      421 cgataatcgc gcaggccgta tttggcctca taggtgtagt cggcgtggcc cgggcgaaac
      481 ttgtccttga tgtcggaata atccttcgag cgctgatcgg tgttctcgat cagaagcgcg
      541 atcggcgtcc cggtcgtcac ctgcgcgccg gtctccggat gcgccatcac gccggacaga
      601 atctttaccg cgtcggcttc ctggcgctgg gtggtgaagc gcgactgccc gggccggcgg
      661 cggtcgagat cgtgctggat gtcttcgact gcgagcggca aaagcggcgg gcagccatcg
      721 accacgcagc cgatcgcgac cccgtggctc tcgccgaagg tcgtgacccg gaacatgtgg
      781 ccgaaggtgt tgaaggacat cgcgttgagc tcggtcggca agcaacaatc aatattgtcg
      841 taacgcgcgc ggttcgagcg gtcaaatgac ctctcgttcg tcatggccgg gcttgtcccg
      901 gccatccacg tctaacttgc agcgcgttcc caaaaaaggc gtggatgccc gggcataggc
      961 gagcggaagc gacgccgtcc ttcggacggg ctatgcccgg ggcatgacgg tggatgggtt
     1021 acgcaccgtc ggcccttaac catacttctc catgccgccg tcgctgaaga cgtagacgac
     1081 gccctgcccg atcggcgttt cggcggcctc gagcgggttt gcgatgccga gcgcgaccat
     1141 cagcgcccgc atggtaccgc catgggcgac cgcgacggta tcggcgacaa gcgaatcgaa
     1201 ccactcgcgc acgcgcaacg tgacgctggc atagctttcg ccggagggcg ccgcgacgcc
     1261 ccatttgtcc tcattgcggg atgcaaaggt cgcagcgtct tgctgctgca tctcgggcag
     1321 cgtcaagcct tcccactggc cgtagccgat ctcgcgcagc cggtcgtcga tcgcgtaatc
     1381 gcccggcggc agctttatcg cgccgcggac cagttccatc gtcgagcgcg cgcggccaag
     1441 cgggctggcg acaaacggca gcgacgacgg atcgcgcccg tcccgcgaaa gcacaccggc
     1501 aagaatgcca cctgatgtca cggcctgggt gcggccgaga tcgttgagcg gaatatcctg
     1561 cgatccctgg aagcgtcccg ccgcgttcca ttccgtctcg ccatggcgaa tgtagtagat
     1621 cgtcggcgcc ggcatcgtaa gcttgattat tccttgctca acgaattgct cagcgagata
     1681 tccggcgcgt ccgggcgctt catgccgacg acgtgataac cggaatcgac gtgatgcacc
     1741 tcgccggtga cgccgcgcga cagatccgac aagagataca tcgcgctgtc gccgacttcc
     1801 tctgttgtga cggtgcggcg catcggcgcg ttgtattcgt tccacttcag aatatagcgg
     1861 aaatcgccga tgccggacgc cgccagcgtc ttgatcggcc ccgccgaaat cgcgttgacg
     1921 cgaatgttct tctcgccgag atcggcggcg agatagcgca cgctcgcctc cagcgccgcc
     1981 ttggcaacgc ccatcacgtt gtaatgcggc atccatttct cggcgccgta ataggtcagc
     2041 gtcaggatcg agccgccgtc ggtcaggagc ttctcggcgc gctgcgcgat cgcggtcagc
     2101 gaatagcagc tgatcagcat ggtcttggaa aaattgtcgg cggtggtttc gagatagcgg
     2161 ccgtcgagtt ggtccttgtc ggagaacgcg atggcgtgca ccacgaaatc gatcttgccc
     2221 catttttccc tgagcacgtt gaataccgca tcgatggttg cgggatcggt gacgtcgcaa
     2281 tggcctagca gaatgccgcc gagttccttc gccagcggct cgacccgctt cttcagcgcg
     2341 tcgccctgcc aggtgaaggc gatttcggca ccggccgcat ggcaggcctt ggcgataccc
     2401 caggcaatcg agcggctatt ggcaacgccg aggatcactc cgcgcttgcc ctgcatcagc
     2461 cctgaacttt gcgacatccg gtttccaatc gaccttgatc aggtctgggg gtacaccagc
     2521 acttccactc ggtacagccc aaatccgggc ttccgttccc tgttgttggt cttgcagcct
     2581 taacagccgg aatagtggtg tcatttcggt gttatcttgg tgtcaggcgt tgccgataca
     2641 ggacgccagg cggacaaccc acgatcgaga tgagatgacc gcgttccgtc aaagtatcga
     2701 agccatgatc ccggcgctgc gccgctatgc acgcgcgctg gcgcgcgatg ccgatatcgc
     2761 cgacgatcta gtgcaggaca cgctggtgcg ggcgctgcgc tcggaacggc tgtttctcgg
     2821 cggcgacgtc agaagctggc tctacaccat cctgaccaac ctcaacaaga acaggcggag
     2881 gtcgctggcg cggcggccgc aattcatgcc gctgctcgac aacaatcccg acgccagcgg
     2941 aaccgaagcg gaaggccgcg acattgcccg cgccctcgca accctggtgg aagagcagcg
     3001 cgcggtgctg ctcctggtga tgctggaagg attgagctac cgcgaggtcg ccgacattca
     3061 gggcgtgccg atcggcaccg taatgtcccg gcttgcgcgc gcccgcgccc atgtgaaagc
     3121 ctcgctcgaa ggcgagcgcg tcgcgctgcg gcgggtgaaa tgatgacagc tatgctgcga
     3181 gccgttgtga tgcgtcagaa acctgaccgg gtatccgtgc gtaatcatat cgtcgtttcg
     3241 ccgcagctgc ggcggtgcgg ttgcaccgca ggcatcggtg agcctcgaag atgacagaga
     3301 tcgggagtaa ggcggtgtct ttcgaggcgc gttcggtcag cggcgccatc gcgccgccga
     3361 cgctgcgctg ctccagcggc gacggcggag ccaacacgca gcgatgacgg caaaccgcaa
     3421 ggacattggt cagatacgac gttgaccaga cacgacagag aagacaaacg acaatgaccg
     3481 acccaaatat ctccatcacc gaagacgaac tgcacgccta cgtcgacaag gaactgccgg
     3541 cggaacgtcg cggcgatgtc gaggcatggc tcgcggcaca tcccgatgat gccgagcggg
     3601 tgcaatcatg gcgcgcgatg gcggaagcgt tgcatgcgcg ttacgattcc gtcgccgacg
     3661 aggcggtgcc gaagcggctt gagatcgagc ggctggagcg gcagccgcgc aaatggctct
     3721 acggtgcgat tgccgcgacg ctgctggcgt tcgtcggcgg cggcggcgtc ggctggctgg
     3781 cgcgcggcgc ggcggcctcg ccgtcggcgt tccagaaact gaccgtggat gcgctcgacg
     3841 cgtaccggct ttatgtcgtc gaggttcgcc acccggtcga ggtgcccgga agcgaacgca
     3901 cccatctcca gcagtggctg accaagcgtt gcggctggga cgtccatgcg ccggagctgg
     3961 acgcgaccgg cttgaagctg gttggcgggc ggctgttgcc cggcccgacc ggcccggcat
     4021 cgttcttgat gtatgagagc gcatcgggcg aacgcttcac gctttacacc tcacgcgcga
     4081 aaaccgggac ggcgcagatg cgctacaccg cggccgagaa cagcggcgcc atgtactggt
     4141 cggaagacgg cgtcggctat gtactatccg gcccgatcga caaggatcgg ctcaatcagg
     4201 tcgcgcggct ggtctacgac cagaccgaaa agacccccgg atgaactatg tcacggcctc
     4261 tgtcccaatt tcgacatcga aaatctcgaa ttggaacatg cgtgcgtctc aatatcgcac
     4321 caagcatcac gcgaaattga gtcgcgttcg atccgccgat cgacgccgcg gcctcgatcg
     4381 gggtttgccg cgctggtccc ccggtcgagg ctgcactttt tttctatttg tggtcccctg
     4441 atccgcccct gcaccatttc gggtggcgcc attcgggtga cggcgcgatc aagtcatgtc
     4501 gctgcgcgga ttgtaagggt gtccgtcgcg ccacttttgc atcaacgcct ccagctcggc
     4561 atcgttgccg tccggcagca tgattcgcgt ggtgacgaag agatcgcctg tcattccagt
     4621 cgctttcggc agccccttgc ccttcagacg aaaggtgcgg ccgctcgagg tgttcttcgg
     4681 tatcgacaat tcgaccgcgc tacccaaagt cggcacgcga accttgccac cgagcaccgc
     4741 ttcatacagc gtgatcggca gatcgacgcg cagatcgctg ccgtcgacct tgaagatcgc
     4801 atgcggcgcg atgctgacgg tgatcaggag atcgccgggg cggtgaccgg gcgcggtctc
     4861 gccttgcccc ttcagcctga tctgctggcc ggcaacgact ccggcaggaa tcttgacatc
     4921 gagttccttg ccggtcggca accggacccg cttcgtcacg ccgtggaccg cttcttccag
     4981 tgacacggtc atggtgacgt tgagatcaag atcgatcgcg atgccgccgg ggtcgaactc
     5041 aaagctgcta ccggcgccgg gtcgcgcgcc gcgtgccgca gcaccgccga acatgctgtt
     5101 gaggatgtcc tcgaaaccgc cgc
//
LOCUS       DATNOI010000269        11390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1830583,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000269 DATNOI010000000
VERSION     DATNOI010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11390
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830583"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VN968_10485"
     CDS             <1..1071
                     /locus_tag="VN968_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HXL29701.1"
                     /translation="SFQMSFAATLGLVALVQIGMPRLFASPDNSATARVALWGGREIM
                     TLALASFVAGLATTPYAAFHFHRVTPYGVLANLAAMPVVSAVVMPAGLLGLLAMPFGL
                     DGVFWQIMGWGIDWMIVVTEWVAGLPGAIGRMASFGIGPLIAASAGIVLLGLLRTPLR
                     WAGAAVLVLAVVWALAVPQPDIVISGDGHNVGVRGRDGRLHLMRTAKDAFLVKEWLAA
                     DADPRQPGDASLADGVSCDEAGCVTQLADGGLVALALRPDAWSDDCERAALVVAARQA
                     PPSCSSPVIGQDRLRRQGAMALRRSRDGFAVDAVKPKGIDRPWSPATAGDTEAEPNLA
                     TRPAAPRAVDATPAEADQQTED"
     gene            1224..1424
                     /locus_tag="VN968_10490"
     CDS             1224..1424
                     /locus_tag="VN968_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29702.1"
                     /translation="MDPTKLRMRYLLGIFLAAVVTAAALRHFLHVQVGLAREDIRTGV
                     LITAGIGIFLMIAFFRWSRRRP"
     gene            complement(1432..1926)
                     /locus_tag="VN968_10495"
     CDS             complement(1432..1926)
                     /locus_tag="VN968_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29703.1"
                     /translation="MTGVLPFIIQLQLRSGEICPVSDPVHICRARTQVTNAQLGLMPG
                     VELWRSQMAAVANVLLREIHATTAQQPIMDIDVVLSANKADRSVKIVKEHLILGTDKR
                     DAEEQRDLWLSENPSIKVVKIHRVKREPQTLLTLIGSKHVPRVSITVEYQEPDAADAN
                     VPAE"
     gene            complement(2058..2312)
                     /locus_tag="VN968_10500"
     CDS             complement(2058..2312)
                     /locus_tag="VN968_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29704.1"
                     /translation="MKKAATILASVAALAVTALAVPGSAEARTYRHWHHYYGYALYYD
                     YDHGNMLSPVSYIYPAANWGPFFHRVRHYGPVLPYQPPAY"
     gene            complement(2529..2699)
                     /locus_tag="VN968_10505"
     CDS             complement(2529..2699)
                     /locus_tag="VN968_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29705.1"
                     /translation="MFRFGWLTVTAEDLAVWEKLPNAAFSLVRTTAATDAGEEFRLGA
                     FELREDLSLGEK"
     gene            complement(3062..3478)
                     /locus_tag="VN968_10510"
     CDS             complement(3062..3478)
                     /locus_tag="VN968_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29706.1"
                     /translation="MCDYSLHAVATRPARVGETLISTTFRGTSTRGFASENAPAVAVC
                     MLPGTELAFAENVRYDNRWIWTKSVNFRVGKFGEIDPDVPHRHHDAIEFPDGSHVLVT
                     QLCEGQRATVLQLPVVDRVAEHAQSAANATPRVSVD"
     gene            complement(4061..4771)
                     /gene="lexA"
                     /locus_tag="VN968_10515"
     CDS             complement(4061..4771)
                     /gene="lexA"
                     /locus_tag="VN968_10515"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HXL29707.1"
                     /translation="MLTRKQYELLRFISERLKESGVPPSFDEMKDALDLRSKSGIHRL
                     ITALEERGFIRRLPNRARAIEVIKLPELSASGGGNGRRGFTPSVIEGTLGRVRSSSSI
                     GSEDDGNRPVAVPVMGRIAAGTPIEALQTRSHTISVPPDMLGSGEHYALEVRGDSMVD
                     AGILDGDMALIQRNETAETGDIVVALIDEEEATLKRFRRRGASIALEPANTSYEVRIL
                     PPNRVKIQGKLIGLYRKY"
     gene            complement(4958..6169)
                     /gene="glp"
                     /locus_tag="VN968_10520"
     CDS             complement(4958..6169)
                     /gene="glp"
                     /locus_tag="VN968_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HXL29708.1"
                     /translation="MALMPVTEALSAVLAGAEPLPEETVALDAAHHRVLARDVAARRT
                     QPPQAMSAMDGYAVRAADASNVAARLKVIGEVAAGRPFDKTVGAGEAVRIFTGGVIPD
                     GADAVIIQEDTVAEGGGITITEAAVAGRHIRPAGVDFREGDVLLARGIRLTDRDLSLA
                     AGMNYPELAVRRRPRVAILATGDELVMPGSTPGPGQIVYSNGYALRALARAEGAETID
                     LGIAADTVEATTHGIRRARDSGADILITTGGASVGDHDLVKQSLEAEGVTMAFWRIAM
                     RPGKPMMHGRLGAMQVIGLPGNPVSSYVCGFLFLVPLIRRLSGRSVIHHTSETALLGC
                     SVAANDLRTDYLRARLELRGDGALVATPVDLQDSSLLGNLAAARALVIRAPFAPAAAA
                     GSACELFRLPD"
     gene            complement(6196..6828)
                     /locus_tag="VN968_10525"
     CDS             complement(6196..6828)
                     /locus_tag="VN968_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXL29709.1"
                     /translation="MTRPLLSPGSADALLFDLGRVVLDIDFSKVVACWAGHAGCEPAH
                     LAGRFSWRDEFYQQHEKGEISDAEFFTALRALLGVELSDAQFLEGWNEIFAGEMPGMP
                     QLLARASQRLPLYAFSNTNSAHVEHFSQAYADVLSHFREMFLSSTIGLRKPDAAAYDH
                     VVKAIGVPASRIVFFDDLAENIEGARARGLTAVHVTSPDDVAHALAALGI"
     gene            complement(7030..8163)
                     /locus_tag="VN968_10530"
     CDS             complement(7030..8163)
                     /locus_tag="VN968_10530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HXL29710.1"
                     /translation="MNEATRIRPATAGELGASGEEFQNLHEFVRKARGNLNQNAWDYI
                     VGAAETETTMRRNRMALDEIAFRPRVLRNVARVDASVTSFGRKLRLPVMLAPVGALEL
                     FDPGAAAAVARGAGAFGAAHMLSSVTEPGLEKVAEAAPDALRIYQLYVRGDDAFVEDC
                     VSRAMTNKYAAFCLTVDTAHYSRRERDIAKRYVRASRLRATGGDFQKGLEWRTVKLIK
                     DKYKIPLVIKGIATAEDAAIALDHGVDWIYVSNHGGRQLDHGRGSMQVLPEIAAAVAG
                     RAKIMVDGSFCRGTDIVKAIASGADLVGIGRLQCWALAAAGEAGVLRMLELLEDEVIR
                     CLGLLGVRNFAELDKSYLHAATPTNLPHVFSAFPLLEIEPYRY"
     gene            8643..8840
                     /locus_tag="VN968_10535"
     CDS             8643..8840
                     /locus_tag="VN968_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29711.1"
                     /translation="MSETLLTIRGIESRPVDAALKLGGDGRFRNVPPQFGYWLGIRGR
                     RGFGTQPSTRQDGLDEIRKAV"
     gene            8932..10266
                     /locus_tag="VN968_10540"
     CDS             8932..10266
                     /locus_tag="VN968_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXL29712.1"
                     /translation="MRKNSLFFLGAATGACLTFLVTGPQGAQLVAAARAAAGSDTYSQ
                     LNLFGDVFERIRTNYVEKPDDGKLIESSINGMIAALDPHSRYMNEKAWRDMQETTSGE
                     FGGLGIEVTMEDGLVKVVAPMDDTPAAKAGILSGDLITHINDEAVQGLSLEEAVGKMK
                     GPIDTKTQLKIVRKGVDKPIDISIVREIIRVRPVSFHVEGGEIGYVRITAFNEQTTDG
                     LKKAIAEMSKQIPPEKLAGYVVDLRNNPGGLLDQAVSVSGTFMARGEIVSTRGRTAEE
                     TQRFSARTGDLIKGKPLVVLINGGSASASEIVAGALHDHKRATLIGTRSFGKGSVQTI
                     IPLGTGNGALALTTARYFTPSGHSIQAQGIKPDIEVLQDVPDELKGRAELKGEASMHG
                     HLSAEGAEQAGSQSYVPPAEKDDKALAAAYNLLRGVTVNAGVPASPKTAVPN"
     gene            complement(10263..10796)
                     /locus_tag="VN968_10545"
     CDS             complement(10263..10796)
                     /locus_tag="VN968_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXL29713.1"
                     /translation="MEALAAGHDAAVQMIDTSVVRVHQDGACIADNKHQEMGRSREGL
                     TCKIHAVVDTNGLLVHLALTPGEGHDNRLRSVLLSALLPQTMPLADRGYDADWIGSLP
                     ASRARGPTSRRNAIPKPDQLQPLPASRAQSRRTVLQQDQAVSARRYPNDKLPANYLAF
                     VKLASIRIWLRANESTS"
BASE COUNT         2106 a         3488 c         3696 g         2100 t
ORIGIN      
        1 agcttccaga tgtcgttcgc ggcgacgctt ggcctggtgg cgctggtgca gatcggcatg
       61 ccgcgtttgt ttgcttcgcc cgacaattcg gccaccgctc gcgtcgcgct atggggcgga
      121 cgggagatca tgacgctggc gctggcctcc ttcgtggcgg gactggcgac gacgccctat
      181 gccgcctttc actttcaccg ggtgacgccc tatggcgtcc tcgccaatct ggcggcgatg
      241 cctgtggtat cggcggtggt gatgccggcc ggcctgctcg gcctcctggc gatgccgttc
      301 gggctcgacg gcgtgttctg gcagatcatg ggatggggca tcgactggat gatcgtggtg
      361 acggaatggg tcgcaggctt acccggtgcg atcggccgca tggcgtcgtt tgggatcggc
      421 ccgctgatcg cggcgagtgc tgggatcgtc ctgctcggac tcttgcgcac gccgctgcgc
      481 tgggctggcg cggcggtgct ggtgctcgcg gtggtctggg cgctggcggt gccgcagccg
      541 gatatcgtga tttccggcga tgggcacaat gtcggcgttc gcggtcgcga cgggcggctg
      601 catctgatgc ggacggcgaa ggacgccttc cttgtgaagg aatggctggc tgccgacgcc
      661 gatcctcggc agcccgggga tgcctcgctt gcagacggcg tctcgtgcga cgaggctggt
      721 tgcgtgacgc agctggccga tggcgggttg gtggcgctgg cgttgcggcc ggacgcatgg
      781 agcgacgatt gcgagcgcgc ggcgctggtg gtggcggcga ggcaagcgcc gccttcatgt
      841 tcatccccgg tgatcgggca ggatcggttg cgacgacagg gggcgatggc gctgcggcga
      901 agccgtgacg gatttgcggt cgatgcggtc aagccaaagg gcatcgatcg tccatggtcg
      961 ccggcgacgg caggcgacac ggaggccgaa ccaaacctcg cgacacgccc cgcggcgccg
     1021 cgtgccgttg acgcgacgcc ggcggaagcc gaccagcaga cggaggactg attgctcatt
     1081 tccggtggcg gcaaaaccgg cgccggttcg tgccatcggc gtggcttcgc attgccgcaa
     1141 aattgcaaac acgggcagag gaaaacgcgt tcaacctcag gagtgtcttg aaaggtaatc
     1201 tgtgccggtg ttagatctga acgatggacc cgaccaagct tcggatgaga tacctgctcg
     1261 gtattttcct tgcagcggtg gtgaccgccg ccgcgttacg ccatttcctt cacgttcaag
     1321 tcggcctcgc ccgcgaagat attcgcaccg gagttttgat aactgccggg atcggcatat
     1381 ttctcatgat cgcattcttc cgatggagca ggcgtcgtcc gtgaacggct ttcattccgc
     1441 gggaacgttg gcgtccgcgg cgtcgggctc ctgatactcc accgtgatcg aaacgcgcgg
     1501 gacatgcttg ctgccgatca gcgtcagcaa ggtctgcggc tctcgcttca cccggtgaat
     1561 cttcacgacc ttgatcgatg gattttccga cagccagagg tctctttgtt cttctgcatc
     1621 gcgcttgtcg gtgcccaaga tcagatgctc tttcacgatc ttgaccgaac gatcggcctt
     1681 attggcagac aggacaacgt ctatatccat gatgggttgc tgcgcggtcg ttgcatgtat
     1741 ttcgcgcaac agaacatttg cgacagcagc catttgcgac ctccaaagct cgacccctgg
     1801 catcagcccc agttgagcgt tggtaacctg agtccgcgcg cgacagatgt gaactgggtc
     1861 actgaccgga caaatctcgc ccgaccgcaa ttgcagttgg atgatgaaag gcaggacgcc
     1921 ggtcatgccg gccggcctgg ctggcatgct tatccggcga tgagagtgac cgatcgtcgc
     1981 cttaagcgcg cgctatccgc aggcgcgcgc tatctggaag gcggccggag agcccggcgt
     2041 ttcgacgaag cggcagtcta ataggcgggc ggctgatagg gcagaaccgg gccgtagtgg
     2101 cgaacccgat ggaaaaacgg gccccagttc gcggcggggt agatgtagct cacaggcgat
     2161 agcatgttgc cgtggtcgta gtcgtagtac agcgcgtaac catagtagtg atgccagtgc
     2221 cggtaggtgc gcgcctcggc cgaaccgggg accgcaagcg cggtcaccgc aagcgcggca
     2281 accgaagcca aaatcgtagc tgcctttttc atcgtcgatc cttcaaaaaa aggcaccgag
     2341 ccaagccaac ggtacaccag cgcaatcgtt tcgacccgtg gtgattccgt ccgccttcgg
     2401 tgcggcgtcg acgttaccgt ttcgcggcgc gcgcgccaag cggtctctcg gcagcgcgac
     2461 gccagccgga agccagcctg cagcaggccg atggacgcgg tgttgaaccg atatttcccg
     2521 aaaatgcttc acttctcacc gagcgagaga tcctcccgca gttcgaaggc gcccagccga
     2581 aactcttcgc cggcgtccgt cgccgccgtc gtccggacca gcgaaaacgc cgcattcggc
     2641 agcttctccc agaccgccag atcttccgcg gtaaccgtga gccacccgaa ccggaacatg
     2701 tatcggcccg gttcggttac tcggccggct tgttgccatg tgactttatc gaccaatcat
     2761 tttttccatc tgaagtcggc aagttttccc gctgaagtct gtaaccgaca tcggccggcc
     2821 agaccggcgt tccagccgtc gtgccggtcc tgaacggttg tttgcgcgtc cgcatttcga
     2881 aggcgtcgag ctgcaaggca aatgtcgcca gcgccagcct cagattattg agctctttgg
     2941 ctcgcgcgtc atcgcgatga cccgtagcgc cgtcgcctgt ggttcgatgc gggccaatat
     3001 tcattgacgc tgctcgctgc caccccgccg ggcgcaaccg gcgggggtcc aaacgaatgt
     3061 tttaatcaac ggagacgcgc ggcgttgcgt tggcagcgga ctgcgcatgc tccgcaaccc
     3121 gatcaaccac cggcaattgc aggacggtcg cgcgttgccc ttcgcagagc tgcgtcacca
     3181 gcacatggct tccatctgga aactcgatcg catcatggtg acgatgcgga acatccgggt
     3241 cgatctcgcc gaatttccca acccggaaat tgactgattt cgtccagatc catcggttgt
     3301 cgtatcggac gttttcggcg aacgccaact cggtcccggg aagcatgcag accgccaccg
     3361 cgggcgcatt ttccgaggcg aaaccacgag tcgaagttcc gcggaatgtt gtggagatga
     3421 gcgtctcgcc aacccgggcg gggcgcgttg ctacagcgtg cagactatag tcacacatcg
     3481 gcttgctcct cgttcgagat agcaggcccg cgccgcctag aggcgcaggc ctctatcaga
     3541 gtggacagta gcacgtgagc ctttgcccgg ttgtaggcaa atgtgcggct ccggccgacg
     3601 gggctgatca caaatatgtt ttaaggaggc tggaccgacg cagggcaggg atgcgtgccg
     3661 caggaagccg atccgcaggc gcatccgcgg gcaaggcgct gaatttgtga gcaccgcctg
     3721 tcgccgggcc ggcgttgctc tcgctgaacc aaaatttgcc gtcgccacct gcgggcaagc
     3781 ggggcggtca agtaaacgcc cgagccgcga ttcccacgaa cgttgcggaa tagacgttcc
     3841 ggaataccgg atgccacacc ggagcctgtc atcgggcccg ccgaaggcgg agacccggtg
     3901 gcggcgcatg accgcagagg tgagtggcgc ggcgatcagc gagacgtcaa tcgccgaccc
     3961 cgcgatcggc ctgtcccagg tcttttcgag atgggtcttt tcgagatgga tctttttggg
     4021 acgcggcttt tcggggtgga cttttcggga tcatgcccgc tcaatacttc cggtaaagcc
     4081 cgatcagctt gccctgaatc ttgacccggt tcggcggcag gatgcggact tcataggagg
     4141 tgttggcggg ttccagcgcg atcgaggcgc cgcggcggcg gaagcgtttc agcgtggctt
     4201 cctcttcgtc gatcagcgcc accacgatgt cgccggtctc ggcggtctcg ttgcgctgga
     4261 tcagcgccat gtcgccgtcg agaatcccgg catccaccat ggaatcgccg cgcacctcga
     4321 gcgcgtagtg ctctccggaa ccgagcatgt ccggtggcac actgatggtg tgactgcggg
     4381 tctgcaacgc ctcaatcggt gtgccggcgg cgatgcgacc catgaccggc acggcaaccg
     4441 gacggttgcc atcatcctcg gagccgatgc tggaactgct gcggaccctg ccgagcgtgc
     4501 cttcgatgac gctgggcgta aagccgcgac ggccgttgcc gccaccgctg gccgaaagct
     4561 cgggcagctt gatgacctcg atggcgcggg cgcgattggg aaggcggcga atgaagccgc
     4621 gctcttcaag cgccgtgatg aggcgatgga tcccggactt cgagcgcaaa tccagcgcat
     4681 ccttcatctc gtcaaaggag ggcggaacgc cggattcctt caggcgttcg ctgatgaatc
     4741 gcaggagttc gtattgtttg cgcgtgagca tctcgaccaa tccccaggtt tgacgtcgtt
     4801 cgattccgag acgcgggagt tagctattcc caattccaag cgtctgatac tcgaaacaaa
     4861 tcatgaacgg accctatatg ttccatatgt gttccgcaac cacttaattt tgcgtcaacg
     4921 aagccaggtt tcgcgaatgc gctgcctaaa atggggatca atccggaagc cgaaacagtt
     4981 cgcaggccga tccggcggcg gcggccgggg cgaacggcgc acggatcaca agcgcccgtg
     5041 ccgcagcgag atttcccaac aacgaagagt cctgcaggtc gaccggcgtg gcgaccaggg
     5101 cgccgtcgcc gcgcagctcg aggcgggcgc gcagatagtc tgtgcgcaaa tcgttggcgg
     5161 cgacgctgca tccgagcagc gccgtttcgc ttgtgtggtg gatgacgctg cggcccgaaa
     5221 gcctgcgaat cagcggcacc aggaacagga atccgcagac gtaggacgat acgggattgc
     5281 cgggcaggcc gatcacctgc atcgcgccca gccgtccatg catcatcggc ttgcccgggc
     5341 gcatcgcgat ccgccagaac gccatggtga cgccctcggc ttccagcgat tgcttgacga
     5401 ggtcgtggtc gccgaccgag gcgccgccag tcgtgatgag gatgtcggcg cctgagtcgc
     5461 gggcgcggcg gatgccatgc gtggtggcct cgacggtgtc ggcggcaatg ccgagatcga
     5521 tggtctcggc accttcggcg cgcgccagcg cccgcagcgc gtagccgttg gaatagacga
     5581 tctgcccggg gccgggggtc gagcccggca tcaccagttc gtcgccggtg gcgaggatcg
     5641 cgaccctggg tcggcggcgc accgcgagtt cgggatagtt catgccggcg gccagcgaca
     5701 ggtcgcgatc ggtgaggcgt atgccgcgtg ccagcaggac atcgccctcg cgaaagtcga
     5761 cgccggcggg gcggatatgc cgtccggcga ccgcagcttc ggtgatggtg atgccacccc
     5821 cctcggccac ggtgtcttcc tggatgatga cggcatcggc accgtcgggg atcacgccgc
     5881 cggtaaaaat ccgcaccgcc tcgccggcgc caacggtctt gtcgaacggg cggcccgccg
     5941 cgacctcgcc gatcaccttg agccgcgccg ccacgttcga cgcgtcggcc gcgcgcaccg
     6001 catagccgtc catcgccgac atcgcctgcg gcggctgcgt gcgccgtgcc gcgacgtccc
     6061 gcgccagcac gcggtgatgc gccgcatcga gcgcgaccgt ctcctcgggc agcggctcgg
     6121 cgcccgcgag aaccgccgac agcgcctcgg taaccggcat cagtgccaag ggagaaactc
     6181 ctaaagccaa aacttctaga ttccgagcgc ggccagcgcg tgcgccacat cgtcaggtga
     6241 tgtcacgtga acggcggtca agccccgcgc ccgcgcgcct tcgatattct cggcgagatc
     6301 gtcaaagaac acgatacggg aggccggtac gccgatagct ttcaccacat gatcataggc
     6361 cgccgcgtcg ggtttccgga gcccgatcgt cgacgacagg aacatttcgc ggaaatggct
     6421 gagcacatcg gcataggctt gcgagaaatg ctcgacatgg gcgctgttgg tattggagaa
     6481 ggcgtagagc ggcaggcgct gcgatgcccg cgccagcagc tgcggcatgc caggcatctc
     6541 gccggcgaag atctcgttcc atccttccag gaactgggcg tcagacagct cgacgccgag
     6601 cagggcgcgg agtgcggtaa aaaattcggc gtcgctgatc tcgccctttt cgtgctgctg
     6661 atagaattcg tcgcgccagg aaaatcgccc cgcgagatgc gcgggctcgc atccggcgtg
     6721 accggcccaa caggcgacaa ccttgctgaa atcaatatcg agcacgacgc ggccgagatc
     6781 gaacaacagc gcatcggcgg agccggggga gagcaagggg cgggtcattg ggattgtgta
     6841 cgaaaattgg gggatgggag caacggtgtg acgattgtcc gtttgatcca atttgcagat
     6901 tcaagtcaga catctcgccg gatccgaatg ccgagattat ggaccggtcg gtcgcgccgg
     6961 ggcaccggcc tcgtcgaatc tgacccgtgg ccggctgtcg tgacgacccg atcgcgcccg
     7021 gcgccgccgt caatagcgat acggttcgat ctccagcagc ggaaacgcac tgaacacatg
     7081 gggcaggttg gtcggcgtcg ccgcgtgcag ataggatttg tcgagttccg cgaaattcct
     7141 gacgccgagc aggccgaggc accggatcac ttcgtcttcg agcagttcca gcatccgcag
     7201 gactccggct tcgccggcag ccgcaagcgc ccagcattgc aggcggccga tgccgacgag
     7261 atcggcgcct gacgcgatcg ccttcacgat atcggtaccg cggcagaacg agccgtcgac
     7321 catgatcttg gcgcggccgg cgacggcggc cgcgatctcc ggcagcacct gcatcgagcc
     7381 acggccatga tcgagctggc gtccgccgtg gtttgagacg tagatccagt ccaccccgtg
     7441 atcgagcgcg atcgcggcat cttccgcggt cgcgatgccc ttgatgacca gcggaatctt
     7501 atacttgtcc ttgatcagtt ttaccgtgcg ccattcgagc cccttctgga agtcgccgcc
     7561 ggtggcgcgc agccggctgg cgcggacgta acgcttggcg atgtcgcgct cgcgccggct
     7621 gtaatgcgcg gtgtcgaccg tcaggcagaa cgcggcatat ttgttggtca tcgcgcgact
     7681 gacacagtcc tcgacaaagg cgtcgtcgcc gcggacatag agctgataga tgcgcaacgc
     7741 gtcgggcgcc gcctcggcga ctttctccag gccaggctcg gtcacggagc tcagcatatg
     7801 ggcggcgccg aaggcgcccg cgccacgcgc gacggcagcg gcggcgccgg gatcgaacag
     7861 ctcgagcgcg ccgaccggcg ccagcatcac cggcaggcgc aatttgcggc cgaacgatgt
     7921 gaccgacgca tcgaccctgg cgacattgcg cagcacccgc ggccggaacg cgatctcgtc
     7981 cagcgccatg cggttgcggc gcatcgtggt ttcggtctcg gcggcgccga cgatgtagtc
     8041 ccaggcgttc tggttcaggt tgccgcgcgc tttccgcaca aactcgtgaa ggttctggaa
     8101 ttcctcgccg cttgcgccga gttcgccagc cgtcgccggc cggatgcggg tcgcctcgtt
     8161 catagtatcc tctcccggaa aacgcgtggg ttcttgttgg gcttggcctt tcgcgtatcg
     8221 cttccagagc ttctgcgcca tcgcaaaaat tggtatggct ggtatcggcg ggacccaagc
     8281 aaggtattcc cggtctgccg aagcggagaa atggccatgt caaaaccggc ggtatggatg
     8341 acgctcggcg taagttaaca ttgtgtttag cgcggagccg atgatcgaaa gccgccatct
     8401 cggtttcgcg cagccggcgg ccggcgatca tcggtcgcga cggggcgaca tccagttcgg
     8461 ctcgggccga ttacaaattg gacagcaaac gagcgatttc gggctttgcc ggcgtcgcgg
     8521 accacgtata tcgtctcact ccagaagacc gcccggcgcg aagccgcgat aacggggtta
     8581 tcccgggata acgggttatc ccgggataac caggcccgac gccaaaacgg ggaaatgaca
     8641 agatgtctga gacgttgctg actatccggg gcatcgagag ccggccggtg gatgccgcgc
     8701 tgaaactcgg cggcgacggt cggttccgca acgttccgcc gcaattcggc tattggctgg
     8761 gtatccgcgg caggcgcggt ttcggcaccc agccctcgac gcggcaggac ggcctcgatg
     8821 agatccgaaa ggcagtgtga gtttatgggt cgagtcgagc accgcgtaag acaccgattt
     8881 atcctgccca tggcgggcgg cagcaactga cagcaggaag gttcgcaaac aatgcgcaag
     8941 aactccctct ttttccttgg cgcggcaacc ggagcatgcc tgacctttct ggttaccggg
     9001 cctcagggcg cgcaattggt tgcggctgcc agggctgccg ccgggagcga cacctattcg
     9061 cagctcaatc tgttcggcga cgtgtttgag cggatcagga ccaactatgt tgaaaagccc
     9121 gacgacggca agcttatcga gtcgtcgatc aatggcatga ttgcggcgct ggaccctcac
     9181 tcgcggtaca tgaatgagaa ggcctggcgc gacatgcagg agaccaccag cggcgagttc
     9241 ggcgggctgg gcatcgaggt tacgatggag gacggcctcg tcaaggtcgt tgcgccgatg
     9301 gatgacacgc ccgccgccaa ggccggcatc ctgtcgggcg acctgatcac ccatatcaat
     9361 gacgaagcgg tccaggggct cagcctggag gaggctgtcg gcaagatgaa ggggccgatc
     9421 gacaccaaga cccagctcaa gatcgtccgc aagggtgtcg acaagcccat cgacatttcg
     9481 atcgtgcgcg aaatcattcg cgtgcgtccg gtcagcttcc acgtcgaagg cggcgagatc
     9541 ggctatgtca ggatcaccgc attcaacgaa cagaccaccg acggactgaa aaaggcgatc
     9601 gccgagatgt cgaaacagat cccgccggaa aagctggcgg gctacgtcgt cgacttgcgc
     9661 aataacccgg gcggactgct cgatcaggcc gtctcggtgt ccggcacatt catggcgcga
     9721 ggcgagatcg tctcgacgcg gggccgcacc gccgaggaga cccagcgttt cagcgcgcga
     9781 accggggatc tcatcaaggg caaaccgctc gtcgtcctga tcaacggcgg atcggcgtcc
     9841 gcatccgaga tcgtggccgg tgcgttgcac gatcacaagc gcgcgaccct gatcgggacc
     9901 cgctcgttcg gcaagggctc ggtacagacg atcatcccgc tcggtaccgg caatggcgca
     9961 ttggcgctca cgaccgcgcg ttattttacg ccgtccggcc actcgatcca ggcccagggc
    10021 atcaaacccg acatcgaggt gttgcaggac gtgcccgacg agttgaaggg ccgcgcggaa
    10081 ctcaagggag aagcgtcgat gcacggccac ctctccgccg aaggcgcaga acaggccggc
    10141 tcgcagtcct acgttccgcc ggcagagaag gacgacaagg cgcttgccgc ggcctacaac
    10201 ctcctgcgtg gcgtcaccgt caatgccggt gtgccggcat ctcccaagac agcggtgccg
    10261 aactaggacg tggactcatt ggcacgcagc caaattcgga ttgatgcaag cttgacgaac
    10321 gccaaatagt tggctggaag cttgtcattc gggtagcgac gcgccgacac tgcttgatct
    10381 tgctgaagaa ccgttcgacg agattgcgcg cgcgatgcgg gtaagggctg aagctgatcg
    10441 ggttttggga ttgcgtttcg gcgggatgtt ggcccacgcg ctctgctggc gggcaagctc
    10501 ccgatccaat cggcgtcata cccacgatcc gcgagcggca tcgtctgtgg aagcaaggcg
    10561 ctgagtagaa ccgaacgtag ccgattgtcg tgcccttcac cgggcgtaag ggccagatgg
    10621 accagcaggc cgttggtgtc caccaccgcg tgaatcttgc acgtcaggcc ctctcgtgag
    10681 cgacccatct cttggtgttt gttgtccgcg atgcaggctc cgtcctggtg gacacgcacg
    10741 acggaagtat cgatcatctg cactgctgcg tcatgaccgg cggccagcgc ctccattatc
    10801 tggtcccaga tgccagcgcg tcgtcagcga aacgattgta gcaagtcgta atcacgtccg
    10861 tcgcggagcc gctaatgctc tgaatttacc tctgctttcg gcggcatagc ggacatggcc
    10921 ggacttgctg ctggctcgat cacgtcgcga atgacccagg ctgtgtgaaa actcgcgcgc
    10981 gggcaaaatg tagagaacac aattcttcta aattgcattt ctggatgcat gagaggcgtg
    11041 cttcctccgt tcagaacgac ggttgagaaa aacattctgc accgtcggcg agcgcgcgag
    11101 ttttcacaca gcctggaccc tttgcggact tatcgaaatc gaaaaagcaa cttaacttgg
    11161 atgggactga agggctgaga cgggcgccac tgcggcctta actacggaag tctttttgtc
    11221 gccgtaaagg gattgaaccc ccgttctgca agggcagccc acgaagctgc gccaagatgt
    11281 ggcaatcgga aatagagtcg tggcttggcc ggatcggtat ccaacatgaa cccagtcggt
    11341 agccctcgga cacttgtggc atagaacccg ccatccggcg acctctggga
//
LOCUS       DATNOI010000270        10162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_830450,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000270 DATNOI010000000
VERSION     DATNOI010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10162
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830450"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VN968_10550"
     CDS             complement(<1..381)
                     /locus_tag="VN968_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL29714.1"
                     /translation="MPIDRSEPEPPGQLRPPGLGLLLAELRGIFEFNASLLLSPLLMR
                     APKGDGHPVLTLPGFLASDLSMAPMRRYLREIGYDSYAWKMGRNIGGVSRLRASLRDR
                     LAEIHAAAGRKVSVVGWSLGGVYAR"
     gene            complement(384..638)
                     /locus_tag="VN968_10555"
     CDS             complement(384..638)
                     /locus_tag="VN968_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HXL29715.1"
                     /translation="MKVNVEIDCTPLEARQFFGLPDVQPMQKAVMDNLQKQMLANIEK
                     VSPEALMQSWFTFDPKLAERFGDMFVTMAGLGGGRSNDKK"
     gene            complement(740..1993)
                     /locus_tag="VN968_10560"
     CDS             complement(740..1993)
                     /locus_tag="VN968_10560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL29716.1"
                     /translation="MAELKADVLVLGAGMVGVSAALHLQQRGRDVILVDRRELAGEET
                     SFGNAGMIESASVFPYMFPRDFGQILQYAINGSPQVRYQFFDLPSFLPWLVRYYLASS
                     PERALRSAMAELPLIRRSLIEHEALIAEAEVPELLRRTGWIKLFRSDATLANAVRDFE
                     RAKQYGVAGELLDGDAIAMREPNLTGEFAGGIHFPAPGFVPDPGGLAKAYAALFKRKG
                     GRFLVGDARTLEQTTGGWRVGGSDGTVVGRETVVALGPWSDLVFGPLGYSIPLGVKRG
                     YHLHLAPRGNAVLNHPVLDSDLGYLLAPMNRGIRLTTGVEFARRDRPPTYIQIEQALP
                     RAQALFPLGDAVDAKPWMGARPCLPDMLPVIGKAPRHAGLWFDFGHQHHGLTLGPATG
                     RLLAEMMTGEAPFADPTPFAVERFG"
     gene            2193..3791
                     /locus_tag="VN968_10565"
     CDS             2193..3791
                     /locus_tag="VN968_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL29717.1"
                     /translation="MSVRPTLLAATTVVIVAFAMSPASAQTLRYANQGSLKSLDPYTL
                     NESTTHAHLGHVYEGLVTRDKDLKTIPALAESWEIPEPTRWRFHLRKNVKFQNGDPFT
                     TDDVVFSAERVQAKGSNLQTRIPAGTKVVKVDDYTVDFVLTSPNPILTAQWDTWYIMD
                     KKWAEANNTVEPTPVAATSPSFASLHANGTGPFTVESHEPGVKTVYKANPNWWRKPEH
                     NLKEIVFTPIASDATRVAALLSGEVDVIEPVPIQDINRVNASAGASVLTGPELRTIFL
                     GMDQVRDELLYSNVKGKNPFKDIRVREAFYKAVDVDLIKNRVMRGLSTPSALMIAPQL
                     FKLSGDFTRPKLDPDGAKKLLTEAGYPDGFEITMDCPNDRYVNDAAICQAIVGMLARI
                     GIKIDLLAQPKAQYFAKVLKPGGFQTSFYMLGWTPSTLDAQNVLYDIMGCRDDLKSPR
                     GEANLGGYCNKQMDAIADKVLLETDTAKRDLLIKEAFEVAAKDFGYIPLHQQALAWGV
                     SKKVKLTQRADNQVLLYWATKQEE"
     gene            3894..4874
                     /locus_tag="VN968_10570"
     CDS             3894..4874
                     /locus_tag="VN968_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL29718.1"
                     /translation="MLAFTLRRAIQAVGVMIAVGIISFSMFRFAGDPVNQIVSLDTSA
                     AERAAISKSLGLDDPVPLQFARYFGNAMRFDFGMSYQFRQPVANLLMERMPATLELAT
                     CATIFAMVFGILMGVYSALKRDTVLAKILQAVSLIGISLPTFLIGILLIYLFAVVLGW
                     LPSFGRGNVVRIGWWTTGLLTVSGLKALIMPSVTLGLFQMTLIMRLVRAEMLEVLRTD
                     YIRFARARGLTTRAIHYGHALKNTLVPVITVAGLQFGSVIAFAIITETVFQWPGMGLL
                     FVQAVQNVDIPIMAAYLLMVSLIFVTINLVVDILYTIVDPRLRSTISRPA"
     gene            4888..5862
                     /locus_tag="VN968_10575"
     CDS             4888..5862
                     /locus_tag="VN968_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL29719.1"
                     /translation="MTEVAFPHKADSSAPPAHAPRGGWFRRALESDVFYSFRRSKLTM
                     AAATITALFFLLAILAPLLAVQNPFDPAQLQLMNSRIAPLWTADGQSPFLLGTDEQGR
                     DVFSAILYGLRISLTVGVLGVLFSGTLGIILGLIAGYVGGAVDGLIMRIADVQLTFPA
                     ILIALLVNGVVKSVFGNRLDAMSTLAVLVFAIGLSFWVQYARTVRGSVMVEKNKDYVA
                     AAQLIGLPAPVIMLRHVLPNATGPILVIATINLALAIITEATLSFLGAGMPDTMPSLG
                     TLIRIGNNYLFAGEWWIVAFPGFALAALIISINLLGDWLRDALNPKLR"
     gene            5894..6880
                     /locus_tag="VN968_10580"
     CDS             5894..6880
                     /locus_tag="VN968_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL29720.1"
                     /translation="MTVPVLSVRNLGVEFATRRSTLRAIDGISFDIAKGEVLGVVGES
                     GAGKSVTGLAVIGLIDPPGDLAGGEIYLSGLRIDNLPPEEMRRIRGKRIGMIFQDPLT
                     SLNPLYRIGDQLVETIRTHLNLSEQAARKRAVDLLAEVGIPAPEKRIDAYPHEFSGGM
                     RQRVVIALAICAEPELIIADEPTTALDVSVQAQIISLIKRLGRDHGTAVMLVTHDMGV
                     IAETSDRVAVMYAGRIAEIGPVQDVVQNPLHPYAKGLMGAIPTLAGDAARLVQIPGSM
                     PRLSAIPPGCSFNPRCAFAFDRCRVDRPEPIRHGAQAVACHLYDAAAKETAA"
     gene            6877..7878
                     /locus_tag="VN968_10585"
     CDS             6877..7878
                     /locus_tag="VN968_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HXL29721.1"
                     /translation="MTSASFIDVRNLRREFDVSKPWLNRVLEGGHLEFLKAVDGVTFD
                     IAKGETFALVGESGSGKTTVARMVVGLLPPSSGEVVIDGVSMSDPRQLQARQQLRRRI
                     QMIFQDPYASLNPRFRVDAIVAEPIRAFDLIHGERNIQARVGELLGLVGLHADDGWKY
                     PHEFSGGQRQRVAIARALASEAEFIVCDEPTSALDVSVQAQILNLMRDLQDKFGLTYL
                     FISHNLAVVRHMANRIGVMYLGRIVEIAEGRELFNSPRMPYTKMLLGAVPDLAMSGRQ
                     RIPVKGEIPNPINPPPGCAFNPRCPLVFDLCRRLAPALIDGVACHAVNNPAHTAVPA"
     gene            7980..9782
                     /locus_tag="VN968_10590"
     CDS             7980..9782
                     /locus_tag="VN968_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HXL29722.1"
                     /translation="MSNINPDPFTTRPEIEGTFGVVTSTHWIATAVGMATLEKGGNAF
                     DAGVATAFTLQVVEPHLNGPGGDVPIIVHDVKRGRTEVICGQGPAPSGATIAHYRSEG
                     LEMVPGTGLLAACVPGTFESWMLLLRDYGTLRLRDVLEPAIAYARDGYPLVERASATI
                     ATVEQMFREHWTTSAAVYLPNGEVPKPGALFTNKRLSETYARILQEAESAGADRVAQI
                     ERARNAWSHGFVAEAIDQFCRTQEIMDVSGSPHRGVLSADDMARWQPSVEAPLTYDYG
                     RYTVCKPGVWSQGPVMLQQLALLKGFELDGLDPAGAEFIHLQTECAKLAFADREKFYG
                     DPKFNEIPIATLLSDAYNDERRKLISDKASLDFIPGSVEGFGSVVKLRRQEGNREAVG
                     AMGAGEPTVGRFGEVRGDTVHFDIIDTAGNMISATPSGGWLQSSPVIPELGFCLGSRA
                     QMFWLDENHPAALAPGKRPRTTLSPSMALRDGEPYLAWGSPGGDQQDQWTTQFFLRHV
                     HARMNLQEAIDAPAWHSEHFPISFWPRTARPGVLVVENRVPKATVDTLKSRGHIIETG
                     PDWSEGRLTAASKVGRCRRAAANPRGMQGYAAGR"
     gene            9789..>10162
                     /locus_tag="VN968_10595"
     CDS             9789..>10162
                     /locus_tag="VN968_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HXL29723.1"
                     /translation="MTWSIIARDNLTGQFGIALATRFFAVGARVPHIAAGIGGIATEA
                     LVNPYYAIDGLKLLREGRKPRDIVETLIAADAGRESRQLHIMDAGGLVASHTGRDCVD
                     WCGHVEGKGFSIAGNMLAGARVL"
BASE COUNT         1874 a         3331 c         3073 g         1884 t
ORIGIN      
        1 acgcgcatag acgccgccga ggctccagcc gacgacactg accttgcggc cggccgccgc
       61 gtgaatctcg gcaagccgat cccgcagcga cgcccgcaag cgcgagacgc cgccgatgtt
      121 gcggcccatc ttccatgcat agctgtcgta tccgatttcc cggagatatc gccgcatcgg
      181 cgccatcgac agatcgctgg cgaggaatcc gggcagcgtc agtaccggat gaccgtcgcc
      241 cttcggcgcg cgcatcaaga gcggcgaaag caacaggctg gcgttgaatt caaatatccc
      301 cctcagctcg gccagcagca ggccgagccc cggcgggcga agctggcccg gcggctccgg
      361 ctcacttcgg tcaatcggca tcgctatttc ttgtcattgc tgcgcccacc gccgaggccg
      421 gccatggtca cgaacatgtc gccaaaccgc tccgcaagct tcggatcgaa cgtgaaccag
      481 ctctgcatca gcgcctccgg agagaccttc tcgatgttgg ccagcatctg cttctggaga
      541 ttgtccatca ccgccttctg catcggctgc acgtccggta gtccgaaaaa ctgccgggct
      601 tcgaggggcg tgcaatctat ttcaacgtta accttcatgt ccactcctgg tcgcatgttg
      661 cccgcctgtc acagtatcgc cacgataggc aacgcgacgc aagctacctg aagggacagg
      721 tatcctgagc cttatatggt cagccgaaac gctccacggc aaagggggtg gggtcggcaa
      781 acggcgcctc gccggtcatc atctccgcca gcaggcggcc ggtagcgggg cccagcgtca
      841 ggccatggtg ctggtgtccg aaatcgaacc acagcccggc atggcgaggc gccttcccga
      901 tcaccggcag catatccggc aaacacggcc gcgcgcccat ccagggcttt gcatcgacgg
      961 cgtctcccag cgggaacaac gcctgcgccc gcggcaaggc ctgctcgatc tggatgtaag
     1021 tcggaggccg atcgcggcgg gcaaattcga cgccggtggt gagacggatg ccgcggttca
     1081 tcggcgccag caaatagccg agatccgaat cgagaacggg atgattgagc acggcattgc
     1141 cgcgcggcgc cagatgcaga tggtatccgc gcttgacgcc aagcgggatg gaatatccga
     1201 gcgggccgaa taccaggtcg gaccagggtc ccaacgccac gacagtttcg cgcccgacca
     1261 ccgtaccgtc ggatccgccg acacgccatc cgcccgtggt ctgctccagt gttctcgcat
     1321 cgcccacgag aaagcgcccg cccttgcgtt tgaacagcgc cgcataggct ttggccaatc
     1381 cacctggatc gggcacgaag ccaggcgcgg gaaaatggat gccaccggcg aactctccgg
     1441 tcagattggg ctcgcgcatc gcgatggcgt cgccatcgag cagttcgcct gcgacgccgt
     1501 actgcttcgc gcgttcgaaa tcccgcaccg cgtttgcaag cgttgcatcg gagcgaaaca
     1561 gcttgatcca gccggttcgc cgcagcaact ccggcacttc ggcttcggcg atcagcgcct
     1621 cgtgctcgat gaggctgcgc cggatcaacg gcaattcggc cattgcgctg cgcagcgccc
     1681 gttccggcga ggatgcgaga tagtagcgca ccagccacgg cagaaagctc ggcaggtcga
     1741 agaactggta acgcacctgc ggtgagccgt tgatcgcata ttgcaggatc tgtccgaaat
     1801 cccgtggaaa catgtaggga aagaccgacg cggattcgat cattccggcg ttcccgaaac
     1861 tggtctcctc gccggcgagt tcgcgcctgt cgaccaggat gacatcccgt ccccgctgct
     1921 gcagatgcaa ggcggcgctg acgccgacca tgcccgcccc caatacgaga acatcggcct
     1981 ttagttccgc catccggtac tcccgttgaa tgtcacgtat ctgctttcgc tgtgtgcacc
     2041 gtagccagtt attgcggcac cgccgcgcaa accggcctgg cgttaaggaa atcgcgcttg
     2101 cgtggcgccc gaactctggg gcaacatgga tgcgcgatag ctgtgtataa tcccgaaaac
     2161 ggacagcgcg ccacagcgga gaacgtgaac aaatgtcggt acgtccaacc ttgcttgcag
     2221 cgacaactgt cgtgatcgtc gctttcgcga tgtcgccggc atcggcccag actctgcgct
     2281 acgccaacca gggttcactc aaatcgctcg atccctacac cctcaacgag agcaccaccc
     2341 acgcgcatct cgggcacgtt tatgaaggcc tggtgacgcg cgacaaggac ctgaagacca
     2401 ttcccgcact ggcggagagc tgggaaatac cggagccgac ccgctggcga ttccacctgc
     2461 gcaagaacgt caaattccaa aatggcgatc ccttcaccac cgacgatgtc gtcttctcgg
     2521 cggagcgcgt tcaggccaag ggctcgaatc tgcaaacccg cattccagcc ggaacaaaag
     2581 tcgtcaaggt cgacgactac accgttgatt tcgtcctgac ctcgccaaat cccattctga
     2641 ctgcgcaatg ggatacctgg tacatcatgg acaagaaatg ggccgaggcg aacaacacgg
     2701 tggagccgac gccggtcgcg gcgacctcgc cgagttttgc ctcgctccac gccaacggca
     2761 ccggtccttt cacggtcgag agccacgagc ccggcgtgaa aaccgtctac aaggcaaatc
     2821 cgaactggtg gcgcaagccc gagcacaatc tcaaggaaat tgttttcacg ccgatcgcct
     2881 ccgacgccac gcgcgtcgcc gccctgctgt cgggtgaggt cgacgtgata gagccggttc
     2941 cgatccagga tattaaccgg gtgaatgcaa gcgccggtgc ttcggtcctg accgggccgg
     3001 aactgcgcac catctttctc ggcatggacc aggttcgcga cgaactgctg tattcgaacg
     3061 tgaagggcaa gaatccgttc aaggatatcc gcgtccgcga ggcgttctac aaggccgtcg
     3121 acgtcgacct gatcaagaac cgcgtgatgc gcggcctttc gacgccgtcg gcgctgatga
     3181 tcgcgccaca gctgttcaag ctctcgggcg atttcacccg accgaaattg gatcccgatg
     3241 gcgccaagaa actcctgacc gaagccggct atcccgatgg tttcgaaatc accatggatt
     3301 gccccaacga ccgctacgtc aatgacgccg cgatctgcca ggcgatcgtc ggcatgctgg
     3361 cccgcattgg gatcaagatc gacctcttgg cgcagcccaa ggcgcagtat ttcgccaagg
     3421 tgctgaagcc cggcgggttc cagacctcgt tctacatgct gggctggacg ccaagcacgc
     3481 tcgatgcgca gaacgttctc tacgacatca tgggctgccg cgacgatctg aaatcgccgc
     3541 gcggtgaagc caatctgggc ggctattgca acaagcagat ggacgcgatc gccgacaagg
     3601 tgctgcttga gaccgacacc gccaaacgcg atttgttgat caaggaggcg ttcgaggtcg
     3661 cggcaaagga tttcgggtat atcccgctgc atcagcaggc cctggcatgg ggggtgtcga
     3721 agaaagtgaa attgacgcag cgcgcggaca atcaggttct gctgtactgg gcgaccaaac
     3781 aggaagaata ggcagcttca ataccaaagt cccggtggct tcgcgccccc gggacttttc
     3841 atttccggcg gacagcgacg tccgccacag caagagaaca gtgaaaggat cacatgctcg
     3901 ctttcactct tcgccgagcc atacaagccg tcggcgtcat gatcgcagtc gggatcatct
     3961 cgttctcgat gttccgcttc gccggcgacc cggtcaacca gatcgtctcg ctcgatacct
     4021 cggccgccga gcgcgccgcg atcagcaagt cgctcgggct cgacgatccg gtgccgttgc
     4081 agttcgcgcg atatttcggc aacgcgatgc ggttcgattt cggcatgtcg taccagttcc
     4141 gccagccggt ggcgaacctc ttgatggagc ggatgccggc aacactggaa ctcgccacct
     4201 gcgccacgat tttcgcgatg gtgtttggca tcctgatggg agtctattcg gcgctcaagc
     4261 gcgatacggt gcttgcaaag atcctgcagg cggtgtcgct gattgggatc tcgctgccga
     4321 ccttcctgat cggcattctc ctgatctatc tgttcgcggt ggtgttgggc tggctgccgt
     4381 cgttcggccg cggcaatgtc gtgcggatcg gctggtggac caccgggcta ttgacggtct
     4441 ccggcctcaa ggccctgatc atgccctcgg tcacgctcgg cctgttccag atgacattga
     4501 tcatgcggct ggtgcgggcc gaaatgctgg aggtcctgcg aaccgactac atccgcttcg
     4561 cgcgggcgcg cggattgaca acgcgggcga tccattacgg tcacgcgctg aaaaataccc
     4621 tggttcccgt catcaccgtc gccgggctgc agtttggctc ggttattgca ttcgcaatca
     4681 ttaccgaaac cgtattccaa tggccgggaa tgggactgct ctttgtgcaa gcggtgcaaa
     4741 acgtcgatat tccgatcatg gcggcctacc tgctgatggt gtccctgatc ttcgtcacca
     4801 tcaatctcgt ggtcgatatt ctctacacca tcgtcgatcc gcgcctgcgc tcgaccatca
     4861 gccgcccggc ctgagcgagg gatcttcatg actgaagtcg ccttcccgca caaagccgat
     4921 tcgagcgctc cgcccgcgca cgcgccgcgg ggaggctggt tcaggcgcgc tctcgaaagc
     4981 gacgtgttct attcgtttcg tcgctccaag ctcacgatgg ccgcagccac catcaccgcg
     5041 ctgttttttc tgctggccat ccttgcacca ttgcttgccg tgcagaatcc gttcgatccg
     5101 gcgcagcttc aactgatgaa ttcacggatc gccccgctgt ggaccgccga cggccaaagc
     5161 ccgttcctgc tcggcaccga cgaacagggc cgcgatgtgt tttccgccat tctctacggc
     5221 ttgcgtattt ctctcaccgt cggcgttctc ggcgtcctgt tctccggcac gctgggcatc
     5281 attctcggat tgatcgccgg ctatgtcggc ggcgcggtcg atggcctgat catgcgcatt
     5341 gccgatgtgc agctcacctt ccccgccatc ctgatcgcgc tgctggtcaa cggcgtcgtc
     5401 aaatccgtat tcggcaaccg cctcgacgcg atgagcacgc tggcggtatt ggtgttcgcg
     5461 atcggtttga gcttttgggt gcaatatgcg cgcaccgttc gcggctctgt catggtggag
     5521 aagaacaagg attatgtggc agccgcgcag ttgatcggcc tccccgcccc cgtgatcatg
     5581 ctgcgccacg tgctgcccaa tgcgaccgga ccgatcctgg tgatcgccac catcaatctg
     5641 gcgctggcga tcatcaccga ggcgacgctg tcgtttctcg gagccggaat gcccgatacc
     5701 atgccctcgc tcggaacgct gatacggatc ggcaacaatt atctgtttgc cggcgagtgg
     5761 tggatcgtcg cctttccggg ctttgcgctc gcggcgctga tcatctccat caacctgctc
     5821 ggcgactggc tgcgcgacgc gctcaatcca aaactccgat gagaaccgcc gcaatttgcg
     5881 agtatctgat cgcatgaccg tacccgttct ctccgtgcgc aatctcgggg tggaattcgc
     5941 cacccgccgc agcacgctgc gcgccatcga cggaatttcc tttgacatcg ccaagggcga
     6001 agtgctgggc gtggtcggcg aatccggcgc cggaaaatcc gtcaccggtc ttgccgtgat
     6061 cggcctgatc gatccgcccg gcgaccttgc cggcggcgag atttatctgt cgggcctgcg
     6121 gatcgacaat ctgccgccgg aagagatgcg ccgtatccgg ggaaaacgga tcgggatgat
     6181 ctttcaggat ccgctgacca gcctcaatcc gctgtaccgg atcggcgatc agctggtcga
     6241 gaccatcagg acccatctca atctctcgga gcaggctgcg cgcaagcgcg ccgtcgacct
     6301 gttggccgag gtcggcatcc ccgcccctga aaagcgcatc gacgcctatc cgcacgaatt
     6361 ctccggcggc atgcgccagc gcgtcgtgat cgcgctcgcg atttgcgccg agcccgaatt
     6421 gatcatcgcc gacgagccca ccaccgcgct cgatgtctcc gtgcaagcgc agatcatctc
     6481 gctgatcaag cgactggggc gcgatcacgg taccgccgtg atgctggtga cccatgacat
     6541 gggcgttatc gccgaaacct ccgaccgcgt cgcggtgatg tatgccggcc ggatcgccga
     6601 aatcgggccg gtgcaggacg tcgtacagaa cccgctgcat ccctatgcca agggcctgat
     6661 gggcgcgatc ccgacgctgg cgggtgacgc ggcgcgtctg gtccagattc ccggctcgat
     6721 gccgcggcta tcggccattc cgccgggctg ctcgttcaat ccgcgctgcg cgttcgcctt
     6781 cgaccgctgc cgggtcgacc ggccggagcc gatccggcat ggcgcgcaag ccgtggcctg
     6841 ccatctctac gacgccgccg cgaaggagac tgcggcatga catcggcgtc ctttatcgac
     6901 gtcaggaatc tgcggcgcga attcgatgtc tcgaagccgt ggctcaaccg tgtgctcgaa
     6961 ggcggccatc tggaatttct gaaagccgtc gacggtgtca ccttcgacat cgccaagggc
     7021 gagacgtttg ccctcgtcgg cgaatccggc tccggcaaga ccaccgtcgc ccgcatggtc
     7081 gtggggttgc tgccgccgag ctcgggtgaa gtcgtcatcg acggcgtttc gatgagcgac
     7141 cccaggcaat tgcaggcgcg ccagcaactg cgccgccgca tccagatgat cttccaggat
     7201 ccctatgcga gcctcaatcc gcgctttcgg gtcgacgcca tcgtcgccga accgatccgc
     7261 gccttcgacc tgatccatgg cgaacgcaac attcaggcgc gggtcggcga gttgcttggt
     7321 ctggtcggcc tgcatgccga cgatggctgg aaatatccgc acgaattttc gggcgggcag
     7381 cgccagaggg tcgcaatcgc ccgcgcgctg gcgtccgagg ccgagttcat cgtctgcgac
     7441 gagccgacct ctgcgctcga cgtctcggtg caggcgcaga tcctcaatct gatgcgcgac
     7501 ctgcaggaca agttcgggct cacctacctc ttcatcagcc acaacctcgc ggtggtccgg
     7561 cacatggcga accgcatcgg cgtgatgtat ctcggccgca tcgtcgagat tgccgaaggg
     7621 cgcgaacttt tcaatagccc gcgaatgccc tacaccaaaa tgctgctcgg cgcggtgccg
     7681 gatctggcga tgtccggccg tcagcggatt cccgtcaaag gcgaaatccc caatccgatc
     7741 aacccgccgc ccggctgcgc gttcaacccg cgctgcccgc ttgtcttcga tctgtgccgc
     7801 agactggcac cggccttgat cgacggcgtc gcctgccacg cggttaacaa tccggcccac
     7861 accgccgtac ccgcatgatc acgatgacga gagcaagaca ccgcagtgga cccgttggca
     7921 ggcagcctcg gctgtggtca attgcgcccg ccacggcggc atcacaacga taggtttcaa
     7981 tgagcaatat caatcccgat ccgttcacga ccaggccgga aatcgaaggc acttttgggg
     8041 tcgtgacgtc cacccattgg atcgccaccg cggtcgggat ggccaccctg gaaaaaggcg
     8101 gcaacgcctt cgatgccggt gtcgccaccg cgtttacgct gcaggtggtc gagccgcatt
     8161 tgaacggtcc cggcggcgac gtgccgatca tcgtgcatga tgtcaagcgc ggccgcaccg
     8221 aggtgatttg cggccagggc cctgcgccgt caggcgccac catcgcgcat taccgcagcg
     8281 aaggcctgga gatggtgccc ggcacgggac tcttggcggc ctgcgttccc ggcacgttcg
     8341 aatcctggat gctgctgctg cgcgactatg gcacgctgcg gttgcgcgac gtgctggagc
     8401 cggccatcgc ctatgcccgc gacggctacc cgctggtgga gcgcgcatcc gccaccattg
     8461 caaccgtcga gcagatgttc cgcgagcact ggacgacgtc ggcggcggtc tatctgccaa
     8521 atggcgaagt gccaaaaccc ggcgcgctct tcaccaacaa aagactttcc gaaacttatg
     8581 cccgtatcct gcaggaagcc gagagcgcag gcgccgaccg cgtcgcgcag atcgaacggg
     8641 cgcggaacgc ctggtcgcat ggctttgtcg ccgaggcgat cgatcaattc tgccggacgc
     8701 aggaaatcat ggacgtcagc ggctcgccgc atcgcggcgt gctcagcgcc gacgacatgg
     8761 cgcgctggca accatccgtc gaagcgccgc tgacctacga ctatggccgc tacaccgtct
     8821 gcaagcccgg ggtctggagc cagggtccgg tgatgctgca gcagctcgcg ctgctgaaag
     8881 gcttcgaact cgacgggctc gatcccgcag gcgccgaatt cattcatctg cagaccgaat
     8941 gcgccaagct tgcttttgcc gaccgcgaga agttttatgg cgacccgaaa ttcaacgaga
     9001 tcccgatcgc gacgctgctg tcggatgcct ataacgacga acgccgcaag ctgatctcgg
     9061 acaaggcgtc gctcgatttc atccccggct cggtcgaagg attcggttcg gtggtaaaac
     9121 tgcgccgcca ggagggcaac cgcgaggcgg tcggcgccat gggcgccggc gagcccaccg
     9181 tcggccgctt cggcgaggtg cgcggcgaca ccgtgcattt cgacatcatc gataccgccg
     9241 gcaacatgat ctcggcgacg ccgtccggcg gctggctgca atcgtcgccg gtgattccgg
     9301 aactcggctt ctgcctcggc agccgcgcgc agatgttctg gctcgacgag aatcaccccg
     9361 cagcgctggc accgggcaag cgcccgcgca ccacgctcag tccgagcatg gcgctgcgcg
     9421 acggcgagcc ctatctggca tggggttccc ccggcggcga tcagcaggat caatggacca
     9481 cgcaattctt cctgcggcac gtgcacgccc ggatgaacct gcaggaagcg atcgacgcgc
     9541 cggcatggca ttcggagcat ttcccgatct cgttctggcc gcgcaccgcc cgtcccggcg
     9601 tgctcgtggt cgagaaccgc gtgccgaagg cgaccgtcga caccttgaag agtcgcggcc
     9661 atatcatcga gaccggtccc gactggtcgg agggccgcct caccgcggcc tcgaaggtcg
     9721 gccgctgccg gcgtgctgcc gccaatcccc gcggcatgca gggctatgcg gccggacgat
     9781 agaccgagat gacctggtca atcatcgccc gcgacaacct gaccggccaa ttcggtatcg
     9841 cgcttgcgac acgttttttc gcggtgggcg cgcgggtgcc gcacatcgcc gccggtatcg
     9901 gcggcatcgc gacggaggcg ctggtcaatc cctattacgc catcgatggt ttgaagttgt
     9961 tgcgcgaagg acgtaagccg cgcgacatcg tcgagaccct gattgcggcg gacgccggcc
    10021 gcgaaagccg gcaattgcac atcatggacg ccggcggact tgtcgcgtcg cataccgggc
    10081 gcgactgcgt cgactggtgc gggcacgtcg aaggcaaggg tttttcgatc gccggcaaca
    10141 tgctcgcggg cgcccgcgtg ct
//
LOCUS       DATNOI010000271         4184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1066259,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000271 DATNOI010000000
VERSION     DATNOI010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066259"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1080
                     /locus_tag="VN968_10600"
     CDS             106..1080
                     /locus_tag="VN968_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL29724.1"
                     /translation="MASRRTAICLIAFGISAIASIGGAAALDYPTRPVRWVVGYPPGG
                     ATDIIARLIGQRLSERLGQQFVIENKPGAGNNIATEAVINAEPDGYTVLLVNPANYIN
                     ASLYTNLKFNVVRDIAPIAAFNRVPNVMTVSNDVPAKTVAEFIAYVKANPGKVNMASS
                     GNGTSVHLSGEMFMMMSGTKMQHVPYRGAAPAITDMLGGQVQLIFDNMPSIIQHVRAS
                     SLRALAVTTTTRSSQLPDVPTVADTVPGYEASALFGMGAPKNTPKEIIAKLNQEINAV
                     LAEPAIKARLIDLGGEPLIGTPDAFGAMIVAETEKWEKVVKAAGVHVE"
     gene            1173..1418
                     /locus_tag="VN968_10605"
     CDS             1173..1418
                     /locus_tag="VN968_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HXL29725.1"
                     /translation="MLVTLVLAAAVAATIAGSGPAAAVDYPYCVQGRGVGIPGDCSYT
                     SYGQCMASASGRGLYCNVNPRVAFGQPQRGRYDRGYY"
     gene            1608..2063
                     /locus_tag="VN968_10610"
     CDS             1608..2063
                     /locus_tag="VN968_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXL29726.1"
                     /translation="MVKYQDHALDRTFAALADPTRRALLARLGKQDGISVSELAQPFA
                     MSLPAIMKHLDVLSDAGLIVRAKTGRIVACRLTAKPMEQAMDWLSHYQRFWSETLERL
                     AAFVEDDEWHPNQALPATPVSSEDRASPSNVVSMPRRPKSTPPGPTRKK"
     gene            2063..2404
                     /locus_tag="VN968_10615"
     CDS             2063..2404
                     /locus_tag="VN968_10615"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXL29727.1"
                     /translation="MRWFGRSDAKPGSFQAEIEARVGGRYRVSFSTDDEYYEVGGIYR
                     EVVPNEKLVFSWAWHSTPERESLVTVTLKPDGDGTLLTLHHEQLFDQAARDGHERGWL
                     GSLDKLERLIA"
     gene            2430..3167
                     /locus_tag="VN968_10620"
     CDS             2430..3167
                     /locus_tag="VN968_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HXL29728.1"
                     /translation="MQPHNIVSREEWIAARKAHLAHEKEYTRARDRLSAERRALPWVK
                     VDKAYWFDGPNGKQTLADLFAGRSQLVVQHFMFAPDWSEGCKSCSFWADGFERMIPHL
                     AARDTTLVAISRAPLQKLDAFKARMGWTFNWLSSADDDFNYDYGVSFTPEQLKSGDKA
                     YNFGTSGFSIEDAPGISVFFRDEAGNIFHTYSCFARGLDMMNAAYHYLDLTPMGRHEE
                     GMPYPMDWLRLRDQYQPAPGTAACCQT"
     gene            3212..3571
                     /locus_tag="VN968_10625"
     CDS             3212..3571
                     /locus_tag="VN968_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HXL29729.1"
                     /translation="MPYVDGFIVAVPKKNLEAYRAMAKKAGKVWREHGALEYCEWVAE
                     DVQVGKRTSFPRSVKLKPGETVIFSWITYKSRAQRDRVNAKVMADKRLAGMMDIKSLP
                     FDAKRMIFGGFESLVKA"
     gene            complement(3579..>4184)
                     /locus_tag="VN968_10630"
     CDS             complement(3579..>4184)
                     /locus_tag="VN968_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL29730.1"
                     /translation="AGSYVALIRDIRFVVPARTAGLLMVGLFAIFSSAPRLLVESFGF
                     SPITLGLLFAAVVFVVFAAGMLATRLSARLGLHRATVVGLAITVIGGVTLLLAGLVAK
                     GSFLPFITATAIFLLGLGIASPLSSAAALSPFGNKAGVAAALLGFAQMAGGALGTLLA
                     AAISSDPALGLGIVAPLASPLALILCRLGARPSGVGPAARA"
BASE COUNT          865 a         1294 c         1291 g          734 t
ORIGIN      
        1 atgtgccgca ctggccgtat cagactttgt ttgttacaac gcgggcgttt gtcaattcaa
       61 cagatgaaac cggacttcag agccgggctt cagggagaat aatgtatggc gtcacgtcgc
      121 accgcgattt gtctgatcgc attcggcatt tcggcaatcg cttcgatcgg cggcgccgcg
      181 gcgctggatt atccgacccg gccagtgcgc tgggttgtcg gatatcctcc gggtggagcg
      241 accgatatca tcgcgcggct gatcggccag cggctctccg agcggctcgg ccagcaattc
      301 gtgatcgaga acaagcccgg cgccggcaac aacatcgcca ccgaagcggt gatcaatgcg
      361 gagccggatg gctacacggt gctgctggta aaccctgcga actacatcaa cgcctcgctc
      421 tacaccaacc tgaagttcaa cgtcgttcgc gacatagcgc caatcgcggc gttcaatcgc
      481 gtgccgaatg tgatgacagt cagcaacgac gtgccggcga aaacggtcgc cgagttcatc
      541 gcctatgtga aggctaatcc gggcaaggtg aacatggcgt catcgggtaa cggcacctcg
      601 gtgcatctgt ccggtgagat gttcatgatg atgtcgggca ccaagatgca gcacgtccca
      661 taccggggcg ccgcgcccgc gatcaccgac atgctcggcg gccaggtgca gctgatcttc
      721 gacaacatgc cctccatcat ccagcatgtc cgggccagtt ccttgcgcgc gctggcggtg
      781 acgaccacga caaggtcttc gcaactgcct gacgtgccga ccgtcgccga caccgttccg
      841 ggctacgaag cgagtgcgct gttcggcatg ggcgcgccga agaacacgcc gaaggagatc
      901 atcgcgaagc tcaaccagga gatcaacgcg gtgctggctg aaccggcgat caaggcccgc
      961 ctgatcgacc tcggcggcga gccgctgatt ggcacgccgg acgcctttgg tgcgatgatc
     1021 gtggccgaaa ccgaaaaatg ggagaaggtg gtcaaggctg ctggtgtgca cgtcgaatag
     1081 gcacgggcgg aaatttcgtc agaagggatc gttcgaacct gagccgacgc aggctgcact
     1141 aatacgcagg aggtgaattc atgcgcaaca cgatgttagt aacgttggtt ttggcggcag
     1201 ccgttgccgc cacgatcgcc ggctccggtc ctgcggccgc ggtcgattat ccctattgtg
     1261 tccagggcag gggtgtcggc attcccggcg actgctctta cacatcttac ggtcaatgta
     1321 tggcgtccgc gtccgggcga ggcctctatt gtaacgtcaa tcctcgcgtt gcgtttggcc
     1381 aaccgcagcg cggacggtat gatcgaggct actactgagg cctgtctgca cgcatccagc
     1441 aattccgccg gcatgtccga cggaattcat acgttgctct gggcagcgcc gtatccatcc
     1501 gccgcggcaa tgcgccggca cggccgccgg ttgtatccga attgtgacga cgggaacgcg
     1561 tcagcgggcg gcttgactct cgctcgctcg cgtctatact aaaccgtatg gttaagtatc
     1621 aagaccatgc tctggaccgc acctttgcgg cacttgcaga cccgacgcgg cgcgcgctgc
     1681 tggcgcggct cggcaagcaa gacggcattt cggtgagcga actggcgcag ccattcgcga
     1741 tgtcgttgcc ggcgatcatg aagcatctcg acgtgctgtc ggacgcaggc ctgatcgtgc
     1801 gtgcgaaaac cggccgtatc gtagcctgcc ggttgaccgc aaaaccgatg gagcaggcga
     1861 tggactggct cagccactac cagcgcttct ggtccgaaac tctcgagcgc cttgctgctt
     1921 tcgtggagga cgatgaatgg cacccaaatc aagcgctgcc ggcgacgccg gtctcgtcgg
     1981 aagaccgagc ctcaccttca aacgtcgtct caatgccgcg ccggccaaag tctacgccgc
     2041 ctggaccgac ccgcaaaaaa taatccgatg gttcggacgc tcggacgcca agcctggttc
     2101 gttccaggcg gagatcgagg cgcgggtcgg cggccgttac cgtgtcagct tctccactga
     2161 cgacgagtat tacgaagtcg gcggcatcta tcgcgaagtg gtgccgaacg aaaaactggt
     2221 gttcagctgg gcctggcatt cgacaccgga acgcgaatcg ctggtcacgg tcacgctgaa
     2281 acccgacggc gacggcacgc tgctcacgct gcaccacgag cagctgttcg atcaggccgc
     2341 ccgcgacggc catgagcgcg gatggctggg gtcgctcgac aagcttgaaa gacttatcgc
     2401 ctgaaaccca tccaacggag gagccggtca tgcagccaca caacatcgtc tcgcgcgagg
     2461 aatggatcgc cgcgcgcaaa gcccatctgg ctcacgagaa ggagtacacc cgcgcccgcg
     2521 accggctcag tgcggagcgc cgcgcgttgc catgggtcaa ggtcgacaag gcctattggt
     2581 tcgacggacc gaacggcaag cagaccctgg ccgatctgtt cgccggccgc agccagctcg
     2641 tggtgcagca tttcatgttc gcgcccgact ggagtgaagg ctgcaagagc tgctcgttct
     2701 gggccgacgg attcgaacgc atgatccctc atctcgcggc gcgcgacacc acgctggtgg
     2761 cgatctcgcg cgcacccttg caaaagcttg acgcgttcaa ggcacgcatg ggctggacct
     2821 ttaactggct gtcgtcggcg gatgacgatt tcaattacga ttacggggtt tcgttcacgc
     2881 cggaacagct caaatcaggt gacaaggcct acaatttcgg cacctcaggc ttcagcatcg
     2941 aagatgcgcc cggcatcagc gtgtttttcc gcgacgaggc gggcaacatc ttccacacct
     3001 attcgtgctt cgcgcgcggc ctcgacatga tgaatgccgc ctatcattat ctcgatctga
     3061 cgccgatggg ccgccacgaa gagggcatgc cgtacccgat ggactggctg cggctgcgcg
     3121 accagtatca gcccgcaccg ggcaccgccg cctgctgcca gacataatcc ataatcgcgc
     3181 gcgacactga ggcataacgg gaggaatgct tatgccttat gtcgatggat ttatcgtcgc
     3241 tgttccaaag aaaaatctgg aggcttaccg cgccatggcg aagaaggcgg gcaaggtctg
     3301 gcgcgagcac ggcgcgctgg aatattgcga atgggtcgcc gaggacgtcc aggtcgggaa
     3361 gcgcacttcg ttcccgcgca gcgtcaagct caagcccggc gagaccgtga tcttttcctg
     3421 gatcacctac aaatcgcgcg cgcaacgcga ccgcgtcaac gccaaggtga tggccgacaa
     3481 acggctggcc ggcatgatgg atatcaagtc gctgccgttc gacgccaagc ggatgatctt
     3541 tggcggtttt gagagtctcg tgaaggcgtg acgcagcctt acgctcgggc ggccggtccg
     3601 acccccgacg ggcgtgcgcc gagccggcaa aggatcagtg cgagcggaga cgccagcggc
     3661 gccacaattc cgagccccag cgccggatcg ctggagatgg ccgccgccag caatgtgccc
     3721 aatgccccgc ctgccatttg cgcgaagccg agcagggcgg ccgcaacgcc agccttgttg
     3781 ccgaatggcg atagcgccgc agcgctcgac agcgggctcg caatgcctaa tccgagcagg
     3841 aagatcgctg ttgcggtaat gaagggcagg aatgaaccct tcgcaaccag cccggcgagc
     3901 agcagcgtga cacccccgat gacggtaatc gccaggccaa ccaccgtggc acggtggaga
     3961 ccgagccgcg ccgataatct cgtcgccagc atgccggccg caaacaccac gaaaacgaca
     4021 gccgcgaaca acaggccaag tgtgatcggg gagaacccaa aactttcaac aagcaggcgc
     4081 ggcgcgctcg agaaaatggc gaacaggccg accatgagaa gacccgcggt cctggccggc
     4141 acaacaaacc tgatatcccg gatcaaggcg acataagatc cggc
//
LOCUS       DATNOI010000272         5634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1204147,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000272 DATNOI010000000
VERSION     DATNOI010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204147"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VN968_10635"
     CDS             <1..289
                     /locus_tag="VN968_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXL29731.1"
                     /translation="KGVNTIAIVSVNDAFVMNAWKRDTDQRDEATFLADGNAEFTKAI
                     GMELDASGNGLGIRSHRYSMLVDDGVVKKFNLEPAPGKVEVSGGDTLLGQL"
     gene            complement(352..804)
                     /gene="rnhA"
                     /locus_tag="VN968_10640"
     CDS             complement(352..804)
                     /gene="rnhA"
                     /locus_tag="VN968_10640"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HXL29732.1"
                     /translation="MSSLPSVTIHTDGACSGNPGPGGWGAILKFGDVEKELKGGEAHT
                     TNNRMELMAAISALETLKKPCTVDLYTDSQYVRQGITGWIHGWKKNGWRTSDKKPVKN
                     VDLWQRLDASLHQHRVRWHWVKGHAGHGENERADQLAREGVAMARLKT"
     gene            complement(801..1784)
                     /locus_tag="VN968_10645"
     CDS             complement(801..1784)
                     /locus_tag="VN968_10645"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HXL29733.1"
                     /translation="MAVYTDVAADELAEFLGGYDIGELLSYKGIAEGVENSNFLLHTT
                     AGYFILTLYEKRVAKGDLPFFLGLMTHLAGHGINCPQPVQNKSGEALSTLAGRPAAII
                     NFLEGIWPRKPNAAHCAGVGQALAKMHLAGRDFGMTRANALSVSGWRPLFDQASSRAD
                     ELQHDLRAFIGAELDHLENNVWPKNLPQGVIHADLFPDNVFFLGEHVSGIIDYTFACT
                     DMLAYDVAICLNAWCFESDYSFNVTKARAFLGGYGRERPLSDAEQDALPLLARGAALR
                     FLLTRLVDFLNVPAGALVRPKDPLEYARKLRFQQGVKTMRDYGVAASGLVA"
     gene            complement(1790..2755)
                     /gene="ispH"
                     /locus_tag="VN968_10650"
     CDS             complement(1790..2755)
                     /gene="ispH"
                     /locus_tag="VN968_10650"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HXL29734.1"
                     /translation="MPAKPPLRIVLCSPRGFCAGVVRAIDTVERALTIYGAPVYVRHE
                     IVHNRYVVDSLKTKGAIFVEELAEIPDNTNAPVVFSAHGVPKSVPADARARNFFSLDA
                     TCPLVTKVHREAAIHFKRGREILLIGHSHHPEVVGTLGQLPQGAVTLIETAHDAATFT
                     PKDPNNLAFVTQTTLSIDDTAEIVAMLKERFPNISGPHKEDICYATTNRQLAVKKVAP
                     VVDALIVVGAPNSSNSQRLREVAEREGCPVSVLAQRAGDLDWNMFEGIKSLGITAGAS
                     APEVIVEEIMGAFAERYELHVETVSAAEENEFFPLPRSLRPDAAE"
     gene            2970..3662
                     /locus_tag="VN968_10655"
     CDS             2970..3662
                     /locus_tag="VN968_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HXL29735.1"
                     /translation="MSNAPLMPKATAVWLVDNTALTFDQVADFTKMHPLEVRAIADGD
                     AAQGIKGMDPISTGQLTRDEIERGEKDVNYRLKLGESKVTLPPAAKKKGPRYTPVSRR
                     HERPSAILWLVRNHPELKDAQIMRLVGTTKTTIASVRDRTHWNASTLTPMDPVTLGLC
                     SQIELDFEVQRAAKEKPIDTAYGGATLLPASETTKKEPEYEPTEKQRDDLNVDAVFAK
                     LKTIGGKKQDEE"
     gene            complement(4545..4763)
                     /locus_tag="VN968_10660"
     CDS             complement(4545..4763)
                     /locus_tag="VN968_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HXL29736.1"
                     /translation="MDIKVRDSNGALLAEGDSVTLIKDLKLKGSSTVLKRGTMIKGIH
                     LTDNADEIEGRTDKVKGLVLRTEFLKKA"
     gene            complement(5040..5246)
                     /locus_tag="VN968_10665"
     CDS             complement(5040..5246)
                     /locus_tag="VN968_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HXL29737.1"
                     /translation="MNEDVFNASLRKFLKKVGITSQREIEKAVRDAMEAGRLKGHEKL
                     PAKMVLTVGGVSLSHEVTDEIELG"
     gene            complement(5262..5531)
                     /locus_tag="VN968_10670"
     CDS             complement(5262..5531)
                     /locus_tag="VN968_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29738.1"
                     /translation="MRRNSARFYLALLLITPLLGGCLERGQPTMVNAGGDDDDAFCRA
                     SNAVGSPEYVACRKDRDVQRNNANAKADRAQRNLGEYMLNHPDHP"
BASE COUNT         1057 a         1776 c         1736 g         1065 t
ORIGIN      
        1 caagggcgtg aacacgatcg cgatcgtttc ggtcaatgac gcctttgtca tgaacgcgtg
       61 gaagcgcgac accgaccagc gcgacgaagc caccttcctc gccgacggca acgccgaatt
      121 caccaaggcg atcggcatgg agctcgacgc ctccggcaac ggtctcggca tccgctccca
      181 ccgctactcg atgctggtcg atgacggcgt ggtcaagaaa ttcaatctcg agccggcgcc
      241 gggcaaagtc gaggtttccg gcggcgatac gttgctgggg cagctgtagc ctccgcaatg
      301 acagccgtgg ttggagcctg tgcctgagaa acaaacgctt cagcccgcca gtcatgtctt
      361 cagccgcgcc atggcgacgc cttcgcgcgc cagttgatcg gcgcgttcgt tctcgccgtg
      421 gccggcgtgg cccttgaccc agtgccagcg cacccggtgc tgatgcagcg acgcatccag
      481 ccgctgccac aggtcgacgt tcttgaccgg cttcttgtcc gaggtgcgcc agccgttctt
      541 cttccagcca tggatccagc cggtgatgcc ctgacggaca tactggctgt cggtgtagag
      601 atcgacggtg cacggcttct tcaaggtttc gagcgccgag atcgccgcca tcagttccat
      661 gcggttgttg gtggtatgcg cctcgccgcc cttcaattcc ttttcgacgt cgccgaattt
      721 caggatcgcg ccccagccgc cgggcccggg attgcccgag cacgcgccat cggtatgaat
      781 ggtgaccgac gggagcgaac tcacgccacc agccccgagg ctgcgacgcc gtaatcgcgc
      841 atggtcttca cgccctgctg aaagcgcagc ttgcgggcat attcgagcgg atcctttggc
      901 cgcaccagcg cgccggcggg aacgttgagg aaatcgacca ggcgggtcag caggaaacgc
      961 aacgccgcac cgcgggccag tagcggcagc gcatcctgct cggcgtcgga caatgggcgc
     1021 tcgcggccat agccgccgag gaaggcgcgc gccttggtga cgttgaacga ataatcgctc
     1081 tcgaaacacc aggcattgag acagatggcg acgtcgtagg ccagcatgtc ggtgcaggca
     1141 aaggtgtaat cgatgatgcc ggatacgtgt tcgccgagga agaagacatt gtccggaaac
     1201 agatctgcgt ggatcacgcc ttgcggcaaa ttcttcggcc agacgttgtt ttcgagatga
     1261 tcgagctcgg cgccgataaa cgcgcgcaag tcgtgctgca gttcatcggc acgcgatgag
     1321 gcctgatcga acagcgggcg ccagcccgac accgacagcg cgttggcgcg cgtcatgccg
     1381 aaatcgcgcc ccgccagatg catcttggcc agcgcctgcc cgacgccggc acagtgcgcg
     1441 gcgttgggct tgcgcggcca gatgccttcc agaaagttga tgatcgccgc cggacggccg
     1501 gccagcgtgc tcaacgcttc accgctcttg ttctgcaccg gctgcggaca attgatgccg
     1561 tggccggcca gatgggtcat caggccgaga aagaacggca ggtcgccctt ggcgacgcgc
     1621 ttctcgtaca gcgtcagaat aaaatatccg gcggtggtgt gcagcaggaa attggaattt
     1681 tcgacgccct cggcgatgcc cttgtaggac agcaattcgc cgatgtcgta accgcccaaa
     1741 aactccgcca gctcgtcggc ggcgacgtcg gtataaaccg ccatcgcgat tattccgccg
     1801 cgtcgggccg cagcgagcgc ggcaacggaa agaactcgtt ctcttccgcg gccgagaccg
     1861 tctccacatg caattcgtaa cgctcggcga acgcacccat gatctcttcg acgatcactt
     1921 ccggcgccga ggcgcccgcg gtgatgccga ggctcttgat gccctcgaac atgttccagt
     1981 cgagatcgcc ggcgcgctgc gccagtaccg agaccgggca accctcgcgc tcggcgactt
     2041 cgcgcaagcg ctgcgagttc gacgagttcg gcgcgccgac cacgatcagg gcgtcgacca
     2101 ccggcgccac ctttttcacc gcgagctggc ggttggtggt ggcgtagcag atgtcttcct
     2161 tgtgcgggcc ggagatgttg ggaaaccgct ccttcagcat cgcgacgatc tcggcggtgt
     2221 cgtcgatcga cagcgtcgtc tgggtgacga aggcgagatt gttgggatcc ttcggcgtga
     2281 aggtcgcggc atcgtgagcg gtctcgatca gcgtcaccgc accttgtgga agctgtccta
     2341 acgtgccgac cacctccgga tggtgggaat gcccgatcag caggatttcg cggccgcgct
     2401 tgaaatggat cgcagcctcc cggtgcacct tggtgaccag cggacaggtg gcgtcgagcg
     2461 aaaagaaatt gcgcgcccgg gcatcggccg ggaccgattt cggaaccccg tgggccgaaa
     2521 ataccaccgg cgcgttggta ttgtcgggga tttcggccag ttcctcgacg aaaatcgcgc
     2581 ccttggtctt gaggctgtcg acgacatagc ggttgtggac gatctcgtgg cggacataga
     2641 ccggggcacc atagatggtg agcgcccgtt ccacggtgtc gatggcccgc accacgccgg
     2701 cgcaaaagcc ccggggcgaa caaagtacga ttctcagggg tggtttggct ggcattaggg
     2761 cgatctcgga ccgaatcacc cctcaccggc atggagggaa cggtcaattc gaataaggac
     2821 ttgggacggc tttcgggcgc tgtcaaggcc ggtcagcaaa ccattgcgcg gttagccccg
     2881 gacggcttat ataggtgata ttcccgtcat cgctgatgac catggcttcg cctccaaaaa
     2941 gggtgggcga agcgcttagg agatatgcca tgagcaatgc accgctgatg ccgaaggcga
     3001 ctgccgtgtg gctggttgac aataccgccc tgactttcga tcaagtggcc gatttcacca
     3061 agatgcaccc gctcgaggtc cgcgccatcg ccgatggcga cgccgcccag ggcatcaagg
     3121 gcatggaccc gatctcgacc ggccagctca cccgcgacga gatcgaaagg ggcgagaagg
     3181 acgtgaacta ccgcctcaag ctcggcgaga gcaaggtgac gctgccgccg gccgccaaga
     3241 agaagggccc gcgctatacg ccggtgtcgc gccgccacga gcggccgagc gcgatcctgt
     3301 ggctggtgcg caatcacccg gaattgaagg acgcgcagat catgcgtctg gtcggcacca
     3361 ccaagaccac catcgccagc gtccgcgacc gcacccactg gaacgcctcg accctgaccc
     3421 cgatggaccc ggtgacgctc ggcctgtgct cgcagatcga gctcgacttc gaggtgcagc
     3481 gcgccgccaa ggagaagccg atcgacaccg cctacggcgg cgccactttg ctgccggcct
     3541 ccgagaccac caagaaggag ccggaatacg agccgaccga aaagcagcgc gacgacctca
     3601 atgtcgatgc ggtgttcgcc aagctgaaga ccatcggcgg caagaagcag gacgaggaat
     3661 aggcggatca attcggcagt ctggatgcga tagcgcaccg ggccgccgca gcaacggtgg
     3721 tcatccccgc gaatgcggga tccactacgc cgcggctgtt cggctccatc accgaccctc
     3781 tggaatactg ggccacccgc cttcgcgggt ggtgacggca cgcgtgtggc gcgcttgtat
     3841 tcaatgaatt gccgcagagc ataaattttc aaaacagcac gttgtttgca gacgcagctt
     3901 cgtttgcaaa cgcagcttcg cgatctcgcc gcgcgtttcg cgcgagtttt gatagcaact
     3961 tgccgccctc tgcgatcaga gggcgtgggg gaacgccggg cgcccttggc acccgcggcc
     4021 tcgtgtgcac tttgtattgg tagaaagcac acgagcaaca acgagtaccc cggaatcacc
     4081 cgacgttccc gcacgcaatg gttttacggc ttacttcgtg ctctccccgg cgaccgggct
     4141 cttttgccac cgtcgtccaa cggattaagg tcttgtccgc gcccggtagg gccgactgac
     4201 ctccgcgaac ttgacaccag catcggggtg tcagaaccac acgacttcac cgtacgcagc
     4261 aacatcgttc gtcagcatgc cgccgattgc tcacaggccg ttcgcagacc tgccctgcca
     4321 ccacgtgtca cgcctgacgc tgccgcgtcc accgcatccc gccccgcgtt cctgacgatc
     4381 gcgaaccgcc cctctgtggg gtgggatggc gcgaatttgt agagatgatt tgcccgacgg
     4441 gtgaagcgaa atatttttgc aaaggggact cgacacacga ttcaaaaagc agcccgacgg
     4501 gcaaatcacc gaccccattc cgacgaaccc ttacgacgtt tctctcacgc cttcttcaaa
     4561 aactccgtgc gcagcaccag gcccttgacc ttgtcggtgc ggccttcgat ctcgtcggcg
     4621 ttgtcggtga ggtgaatgcc tttgatcatg gtgccgcgct tgagcactgt ggacgagccc
     4681 ttcagcttca gatccttgat cagcgtgacg ctgtcgccct cggcgagcag cgcgccgttg
     4741 gaatccctga ccttgatgtc catgcagtgt tgtcctttgt gataaaaaag cgggacgcct
     4801 tttcttccct tctccccttg tgggagaagg tggccgtagg cggccttcgg ccgccgttct
     4861 ttaatagcac gccgatgctt tgcatcggct atgcgaagtc cgcgccggat gaggggttct
     4921 tttctaattg atacgccgcg acccctcacc cgtctcgcat ctgacgatgc gagccagcca
     4981 ccctctccca caagggggcc cacaagggga gaggggaaga aggggcgcgc cgcgttgcgc
     5041 taacctaact ctatttcgtc ggtgacctcg tggctgaggc tgacgccgcc cacggtcaga
     5101 accattttgg ccggcagttt ttcatggccc ttgagacggc cggcctccat ggcgtcgcgc
     5161 accgcctttt cgatttcgcg ctgcgaggtg atgccgacct tcttcaggaa tttgcgcagg
     5221 ctggcattga agacgtcttc gttcatgacg gcctcctgat gttacgggtg gtccggatgg
     5281 ttcagcatgt attcgccgag attgcgctgc gcccgatcgg ctttcgcatt ggcgttgttg
     5341 cgttgaacgt cgcggtcctt gcggcaggcg acatattccg gcgagcccac cgcattgctg
     5401 gcgcgacaga acgcgtcgtc gtcatcgccg ccggcattga ccatggtcgg ctgtccccgc
     5461 tcgaggcagc ccccgagcaa cggcgtgatc agcaatagcg cgagatagaa tcgtgcggag
     5521 ttacgtcgca tgcgcgggtt ccgttcgttg ttcctatttc gtcattgccg ggcttgaccc
     5581 ggcaatccat caaaaagaag gcgtttgctt gagatggatg cccgggtcaa gccc
//
LOCUS       DATNOI010000273         2130 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_11157,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000273 DATNOI010000000
VERSION     DATNOI010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11157"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..498
                     /locus_tag="VN968_10675"
                     /pseudo
     CDS             160..498
                     /locus_tag="VN968_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            764..2098
                     /locus_tag="VN968_10680"
     CDS             764..2098
                     /locus_tag="VN968_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HXL29739.1"
                     /translation="MSQPRDDRQDDLFRSSLEAIINLRHPLVRLAAEINWDFLAKRFG
                     SVCRVGPGQPPLPTRLVAGLFILKHMHSLSDEALCDRWVENPYFQYFCGEVVFRHELP
                     FDRSSLTRWRQRLGEEQIAALLQESLSVAHRSGAIETKDLERVVVDTTVQEKAIAHPT
                     DARLTHRAIEKLVDLAKCEGVELRQSYLRVAKRAAIMVGRYTHAHQFKRARRQLKFLR
                     TRLGRIIRDISRKIEGNTALEDRFGPLLDLASRVRHQGQRQRGPKVYSLHAPEVECIG
                     RGKARAPYEFGCKVSIVTPVTAPNGGQFVLHAKALHGNPYDGHTLGPVIADLEKLTGV
                     AVRGIHGDKGYRGHNYPDRFKVWITGQVRRVTKAVRREMRRRAAVEPVIGHLKEDHRM
                     GRNYLKGRDGDRINAVLAAAGYNFSLLLRWFRRLLRCLLLILAGVVLAPRLA"
BASE COUNT          403 a          654 c          659 g          414 t
ORIGIN      
        1 acctggaaca ctgacttggc aatgtcgaga ccgattgtcg tgatcatctg catggctagc
       61 tcctctgaat cgtgggagcc ttaacagcgc ccacatccgt ggcactctcg tgccggtgga
      121 ggagccgtcc acagcatcaa gtgcggacat tcgcgaatgc tgaagcacat cgccgagttg
      181 ttcgccatcg agaaggacat tcgcggccgc agtgccgagg agcgacgcct cattcgacaa
      241 cagaggccgg ccgctcgccg atgcattcca gaagtggcta cccacaaagc ttggtctgat
      301 cagtcagaag ggcaagctcg ccgacgccat ccgctatgcg ctctcgcgcg ctgggaggga
      361 ctgacgcgct tccttgatga tggccatatc aagctcgaca acagccccgt cgaacggaaa
      421 aatgcgctct tcgcaggctc cgacgacggc gccgagcacc gcgccgtcat cgcttcggtg
      481 atcgaaacct gcaagctgaa cgacggcgat ccgctcgcct aagagctgtt acgttgacgg
      541 ccacgacgtg gactttggga aggcacacgc caggtagcgc tgcttacgtt gtcgcccgtg
      601 aagtatttgg aagtcattga ttgatcggtg tgatttgatc tgaacaagta ttcgatcttg
      661 caagaaagcc ctatgttgat ggcggttttc ttgcgttttg ggattcccga tttgggcggt
      721 tcgtgattcc ttatgacggc gagattcgct gttgggggga acgatgagcc agccgcgtga
      781 cgaccgccag gacgacttgt ttcgctcgtc tctggaggcg atcatcaacc ttcgccatcc
      841 gctggtgcgg ctcgcagcgg agattaattg ggattttctg gcgaagcgct tcggttcggt
      901 atgtcgcgta gggccggggc agccgccgct gccaacgcgg ctagtggccg ggctgttcat
      961 cctcaaacac atgcacagtc tctctgacga agcgctgtgc gacaggtggg tagagaaccc
     1021 gtatttccag tatttttgcg gagaagtggt gttccggcat gaactgccgt ttgaccgctc
     1081 gtcgctgacc cgctggcgcc aacggctggg tgaggagcag atcgccgcgc tgttgcaaga
     1141 gagcctctcg gtggcgcacc ggagcggggc gatcgagacc aaagatctcg agcgggtcgt
     1201 ggtggacacc accgtgcagg agaaagcgat cgcgcatccg accgacgccc ggctcaccca
     1261 ccgcgcgatc gagaagctgg tcgatctggc caaatgcgag ggtgtcgaac tgcgccagag
     1321 ctatctgcgg gtggccaagc gcgccgccat catggtcggg cgctatacgc atgcgcatca
     1381 gttcaagcgc gccaggcggc aactgaagtt cctgcgcacg cggctcggcc gcatcatccg
     1441 cgacatcagc cgcaagatcg agggcaacac agcgctcgaa gaccgcttcg ggccgctact
     1501 cgatctcgcc tcgcgtgtgc gccatcaagg gcagcgtcag cgcgggccga aggtctattc
     1561 gttgcacgcg cccgaggtgg agtgcatcgg ccgcggcaaa gcacgggctc cctacgagtt
     1621 cggttgcaag gtctcgattg ttacgccggt caccgcgccc aacggcgggc agttcgtgct
     1681 gcatgccaag gcactgcacg gcaatcctta cgacggtcac acactcggtc ccgtcatcgc
     1741 cgatctcgaa aagcttacag gggttgccgt ccgcggcatc cacggcgaca agggctatcg
     1801 cggtcacaac tatccggacc ggttcaaggt ctggatcacc ggccaggtcc ggcgtgtcac
     1861 caaggccgtt cgccgcgaga tgcgacgccg ggccgccgtc gagcccgtga tcggccatct
     1921 caaggaagat caccgtatgg gtcgcaacta tctcaagggc cgcgacggcg accgcatcaa
     1981 cgcggttctc gccgccgccg gctacaactt cagcctactc ctgcgctggt tcaggcgcct
     2041 attgcgctgc ctgttgctga tccttgctgg cgtcgtcttg gcgccccgcc tcgcttaaaa
     2101 ccgcgctcgg aaacattctt cacggtcgac
//
LOCUS       DATNOI010000274         6594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1984830,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000274 DATNOI010000000
VERSION     DATNOI010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984830"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1837)
                     /locus_tag="VN968_10685"
     CDS             complement(143..1837)
                     /locus_tag="VN968_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL29740.1"
                     /translation="MGLLSRLKIRTKLASMVVLSALTVCAIIALSASMSRGRMIEDRI
                     GQLHATVDMLVGLAQTLQDEVTAGKMTLAEAQTQFRQRGRKMTFNNGQGYPVVYNADT
                     SIMLNGANPQLEGKITGATDSNGVVIAKAQMEAAQRSPEGGTATYLYPRPGQTIPVPK
                     MVFVRGFAPWNVVMSYGLYIDDLDGDVNALLLRLGAIGAGIMLLMLLLSWLISRDVLG
                     ALDRQRTRMQRIATGSLDQAVEETERGDEIGRMAETLEMLRQTALTARTLEAEQIATK
                     QQGEQEKREALITLADRFDASVGRLVGMMASGSTELEATAQSMTGTAERTNQQATVVS
                     SAAAEASSRVRTVATAAEELSSSISEISRQVAQSADIASRAVDSARRTDTIVRALADG
                     AHQIEHVAELISSIAGQTNLLALNATIEAARAGDAGRGFAVVAAEVKSLASQTAEATK
                     EIGTRIAQIQSATKEAVEAIQGITATIEEVSAIATTIGSAIEEQGAATAEIARNVTQT
                     AQATQEVTTNIGGVSAAANETGGAAGHVLSAASNLSKHAEQLSGEVNVFLAGVRAA"
     gene            complement(1990..3237)
                     /locus_tag="VN968_10690"
     CDS             complement(1990..3237)
                     /locus_tag="VN968_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL29741.1"
                     /translation="MDQDTPRENPETKSPQPARMALNVLALCFALSVFGRGLAESFAV
                     FLKPISESFGWDRAQVVSVYSLAALAGGLSAPLVGRLFDRSGPRTVYSLGLALLGGAF
                     LVAAHAQQLWQFQLSIGLCVGIGIAFIGNVPNSILLGRWFGPRLPTAMAIVYSATGAG
                     VLILLPVSQVLIDRFGWRGAYQIFGGGALFLLLPLLLLPWRLFSTGSPNVVKKADPDF
                     VDQGWTLLGAMRHHAFWALFSTFFFTAIGMYAISPQVVAYLIDAGFPPLQAATAWGFS
                     GVVLLFGMLGISWLDGIIGRRPSVLFSYALSIAGILMLWGLQWYPNFWMLTGFVVCFG
                     SMIGSRGPLLTATAMKIFRGKRIGTIYGTISIGSGLGSALGSWSGGLIHDWTHSYNPL
                     IAFALVSVVLGMIPFLVVPALRR"
     gene            complement(3293..4549)
                     /gene="tyrS"
                     /locus_tag="VN968_10695"
     CDS             complement(3293..4549)
                     /gene="tyrS"
                     /locus_tag="VN968_10695"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HXL29742.1"
                     /translation="MTAFKSDFLNVLRSRGFIHQISDPDSLDALAAKRELVAYIGFDC
                     TAPSLHVGSMVQIMCLYWLQQTGNKPIALMGGGTTRVGDPSGKDATRRILSIEDIERN
                     KEGIKQVFSQHLLEFGDGKHDAVMPDNAEWLTKLNWIEMLRDIGRHFSVNRMLAMDSV
                     KLRLDRDQEMSFIEFNYMILQAYDYVVLNQRYDCRLQMGGSDQWGNIVNGIDLGRRMG
                     TPQLYALTTPLITTANGEKMGKTAGGAVWLNADMLSPYDYWQFWRNTADADVERFLKL
                     FTTLPLDLIADLSALKGQDINQAKIMLANKATELVHGFGAAMSAAATARKTFEEGSLS
                     SDLPTIEISISELDAGIGLLSAVVKAGLAASNGEVRRQIKAGGVRVNDVPVSDEKMVL
                     TPAHLTEEGVIKLSVGRKRHILLRPA"
     gene            4717..5820
                     /locus_tag="VN968_10700"
     CDS             4717..5820
                     /locus_tag="VN968_10700"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HXL29743.1"
                     /translation="MMLTAIGLMSGTSLDGVDVALIETDGRRVKAFGPSGYRPYTDAE
                     RGLLRQALAEAVDLPRRDARPGILREAERAVTVAHAEAVAAFTAQHRIAREDVDIVGF
                     HGQTVLHRPAQKMTVQIGDAAALARAIHIPVMHDFRAADVEAGGQGAPFVPVYHRALA
                     QTLQREGPIAVVNIGGVSNITYIDGTDTLIACDTGPGNALLDDFMFRTMGQRFDCEGR
                     MAAQGAVDVAWVMQALAHPFFALPPPKSLDRNDFASQILRGVSPADGAATLTAFTAEA
                     IARIAPLLPKQPRSWIVAGGGARNLTMLRLLRERLEPATVETAEGLGWSGDAIEAQAF
                     GFLAARGLKGLPLSYPATTGVPIPMTGGVIARP"
     gene            5916..6536
                     /locus_tag="VN968_10705"
     CDS             5916..6536
                     /locus_tag="VN968_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXL29744.1"
                     /translation="MAAPLKLISHKLCPYVQRAVIALNEKGVPFERIDIDLANKPDWF
                     LAISPLGKTPVLQVGDTAIFESAVILEYLEETQPKPLHPADALARAEHRGWIEFGSAV
                     LNDIAGFYAAADEATFNAKTLQLEQRFARLETRVAAAPWFDGESFSLVDAVFGPVFRY
                     FDVFDDIADFGILAGKPKLTQLAQLEVCFCDFARHAASQIKQLDSA"
BASE COUNT         1270 a         2045 c         2022 g         1257 t
ORIGIN      
        1 tttcggccat attcccggga gtctcgcgga aaatctggga gttcaacacc ggcaagcccc
       61 cggctgaatt tccggcgccg aacaacaact gacctcgacg agcttcgacc ggaagccgca
      121 cctatgacga aaaacgaatc cgtcaggctg cgcgcacgcc ggccaggaag acgttgacct
      181 cgcccgacaa ttgctcggcg tgcttggaga ggttagaggc ggcggaaaga acatggccgg
      241 cggcgccgcc ggtttcattg gccgcggcgc tgacgccacc gatatttgtc gtcacctcct
      301 gcgtcgcctg cgccgtctgg gtcacgttgc gcgcaatctc cgcggtagca gcaccttgtt
      361 cctcgatcgc ggagccgatc gtcgttgcga tcgcgctgac ttcctcgatc gtggcggtga
      421 tgccctggat cgcctcgacg gcttccttgg tggcgctctg gatctgggca atgcgggtac
      481 cgatctcctt ggtggcttcc gcggtctggc tggcgaggct cttgacctcg gccgccacca
      541 ccgcgaaacc cctgccggca tcgcctgcgc gggcggcttc gatggtggca ttgagcgcga
      601 gcagattggt ctgccctgcg atacttgaaa tcagttccgc cacatgctcg atctggtggg
      661 caccatcggc aagggcccgc acgatggtgt cggtgcggcg cgcgctgtcg acggcgcggc
      721 tggcgatatc ggcggattgc gcgacctgac ggctgatttc gctgatcgag gaggacagct
      781 cttccgccgc cgtggcgacg gttcgcacgc gcgaactggc ctccgccgcc gccgagctga
      841 cgaccgtcgc ctgctggttg gtgcgttccg ccgtgccggt catggattgc gcggtggctt
      901 ccagttcggt cgatcccgaa gccatcatgc cgaccagccg gcccaccgag gcatcaaatc
      961 gatcggcgag cgtaatgagt gcctcgcgct tctcctgttc accttgctgc ttcgtcgcga
     1021 tctgctcggc ttccagcgtc cgcgccgtca gcgccgtctg ccgcaacatc tccagcgttt
     1081 ccgccatgcg gccgatctcg tcgccgcgtt cggtttcttc caccgcctga tcgagcgatc
     1141 ccgtcgcgat gcgctgcatc cgggttcgct gccggtcgag cgcacccaga acatcccgag
     1201 agatcagcca tgacagcagc agcatcagca acatgatgcc ggcgccgatc gcaccaaggc
     1261 gcagcagcaa tgcattcaca tctccatcga gatcgtcgat gtagagtccg tagctcatga
     1321 cgacgttcca cggcgcgaaa ccgcgcacaa agaccatttt tggaacagga atggtttggc
     1381 cgggacgggg gtaaaggtag gtggcggtcc ctccctcagg actgcgctgg gcggcttcca
     1441 tctgggcctt ggcgatcacg acgccgttgg aatcggtcgc accggtgatc ttgccttcga
     1501 gctgaggatt ggccccgttc agcatgatcg aagtatccgc gttgtagacg acgggatagc
     1561 cttgcccgtt gttgaacgtc atcttcctgc cgcgctgccg aaactgtgtc tgcgcctcgg
     1621 cgagagtcat tttgcctgcc gtaacctcgt cctggagtgt ctgggcgagg ccaaccagca
     1681 tatcaaccgt cgcgtgcaat tgtccgatcc ggtcctctat catgcggcct cggctcatcg
     1741 aagccgacag cgcgatgatg gcgcagaccg tcagcgccga cagcacaacc atgctcgcca
     1801 gtttcgtacg aattttcaaa cggctaagca gacccatttc gaccctccag cgaattcccc
     1861 gcaaggcaag gtatcactac tttcttacca atcatgaatg cgagcgggat tgcggcaggc
     1921 cagccgatat tcgcgaaata ggcgaagaaa caacatcaac ggggccgatc tgggtgtgcg
     1981 ctaatcgatc tatcggcgca acgccggtac aaccaggaac ggaatcatgc ccagcaccac
     2041 gctgaccagc gcgaacgcga tcaacggatt atagctgtgg gtccagtcgt ggatcaggcc
     2101 tccgctccag gaaccgagcg ccgagccgag gccgctgccg atggaaatgg tgccgtagat
     2161 cgttccaatc cgcttgccgc gaaagatctt catcgcggtt gccgtcagca gcgggccgcg
     2221 tgagccgatc atgctgccga aacacacgac gaatcccgtc agcatccaga agttcggata
     2281 ccactgcagg ccccacaaca tcagaattcc agctatcgaa agcgcatagc tgaacaggac
     2341 cgatggcctg cggccgatga tgccgtcgag ccagctgatg ccgagcatgc cgaacagcag
     2401 caccacgccg ctgaaacccc aggcggtcgc cgcctgcagg ggcggaaatc ccgcgtcgat
     2461 cagataggcc acgacttgcg gtgaaatcgc atacatgccg atcgcggtga agaagaaggt
     2521 cgagaacaac gcccagaacg cgtgatggcg catggcgccg agaagcgtcc agccttgatc
     2581 gacaaaatcc ggatcggcct ttttgacgac gttcggggat ccggtcgaga acagccgcca
     2641 cggcaacaaa agcagcggca acagcagaaa cagcgcgccg ccaccaaaaa tctgataggc
     2701 gccgcgccag ccgaagcgat cgatcaacac ctgcgagacc ggcagcagga tcaatacgcc
     2761 ggcgccggtc gccgaataca cgatcgccat cgcggtcggc aatcgcgggc caaaccagcg
     2821 gccgagcagg atcgagttcg gaacattgcc gatgaacgca atgccgatcc cgacgcagag
     2881 gccgatgctc agctggaact gccagagctg ttgcgcgtgg gcggcgacca gaaacgcccc
     2941 gcccagcagc gccaggccaa gcgaatagac ggtgcgcggg cccgagcggt cgaacagccg
     3001 gccgaccagc ggcgccgaca gtccgccggc gagggctgcc agcgaataga ccgagacgac
     3061 ctgggcgcgg tcccagccaa agctctccga gatcggcttg aggaacacgg caaaactctc
     3121 ggcgagacca cggccaaaca ccgacagcgc aaaacacagc gccagcacgt taagcgccat
     3181 ccgtgccggc tgcggtgatt ttgtctccgg attctcgcgc ggcgtatctt gatccatgcc
     3241 caccaaggaa cgcttttcgg cggcgaagag caggggtctt gggcgaatgc gcctatgcag
     3301 gccgcagcag gatgtggcgc ttcctgccaa ccgacagctt tatgacgcct tcctccgtta
     3361 ggtgcgctgg cgtcagtacc atcttctcgt ccgacaccgg aacatcattg acgcgcacac
     3421 cgcctgcttt tatctgacgc cgcacttcgc catttgaggc tgcgagcccc gccttaacga
     3481 ccgccgagag taatccgatg ccggcatcaa gttcacttat cgaaatttcg atggttggca
     3541 gatcgctgga aagggagccc tcttcaaaag ttttccgcgc agtcgctgcc gcagacatcg
     3601 ctgcgccaaa accatgtacg agttctgtgg ctttgtttgc gagcataatt ttcgcttggt
     3661 tgatatcctg cccctttaag gctgaaagat ccgcgataag atcaagcggc agtgtagtaa
     3721 agagcttgag aaagcgctcg acatcagcgt ccgcagtgtt gcgccaaaat tgccaataat
     3781 cataagggct gagcatatcc gcatttagcc aaacggcccc accggccgtt ttccccatct
     3841 tctctccatt cgcggtcgtg atcagcggcg tcgtcagcgc gtacaattgt ggcgttccca
     3901 tgcggcggcc aagatcgatg ccgttgacga tattgcccca ttggtcggag ccgcccatct
     3961 ggagacggca atcgtaacgc tggttcagca cgacgtagtc gtaggcctgc aggatcatgt
     4021 agttgaattc gatgaagctc atctcctgat cgcggtcgag ccgtagcttg accgagtcca
     4081 tcgccagcat gcggttgacg gagaaatgcc ggccgatatc gcgaagcatc tcgatccagt
     4141 tcagtttggt cagccattcg gcattgtcgg gcatgacggc gtcgtgcttg ccgtcgccaa
     4201 attccaagag gtgttgtgag aacacctgct tgatgccctc cttgttgcgc tcgatatcct
     4261 cgatcgaaag aatcctgcgt gtcgcgtcct taccggaggg gtcgccgacg cgggtggtgc
     4321 caccgcccat cagcgcgatt ggcttgttgc cggtctgctg cagccagtag aggcacatga
     4381 tctgtaccat ggagccgaca tggagcgaag gtgcggtgca gtcgaagccg atataggcca
     4441 cgagttcccg cttggctgcc agcgcatcca gcgagtccgg gtccgaaatc tgatgaataa
     4501 agccgcggct ccgcagcaca ttgagaaaat cggatttaaa tgcagtcatt tgtgtgcgac
     4561 tctgatcatt cttacttttt cacgctattc ttaacgggtt gcggaaccat agcggaatag
     4621 gctgccgctg cccggcgcgc tgtggcatta taagatgtgc tggtttgata caagctgggc
     4681 gtcagcggca gtgcgccagc aaaggcacta tttgcgatga tgttgacggc aatcggtctg
     4741 atgagcggca cctcgctcga cggggtcgat gtcgcgttga tcgagaccga tggcaggcgg
     4801 gtcaaggcgt ttggtccgtc gggttacagg ccctacaccg acgccgaacg cggcctgctg
     4861 cgccaggccc tggcggaggc ggtcgacctg ccgcggcgcg atgcgcggcc gggcatcctg
     4921 cgcgaggccg agcgcgccgt cacggtcgcg catgcggaag cggtcgccgc ctttaccgcg
     4981 cagcaccgga tcgcccgcga ggatgtcgat atcgtcggct ttcatggcca gaccgtgctg
     5041 caccggcccg cgcagaaaat gaccgtgcag atcggcgatg cggcggcgct ggcgagagcc
     5101 attcatatcc cggtgatgca tgatttccgc gccgccgatg tcgaggccgg cgggcagggt
     5161 gcccccttcg tgccggtcta tcaccgcgcg ctggcccaga cgctgcagcg cgaggggccg
     5221 atcgccgtgg ttaatatcgg cggcgtctcc aacatcacct atatcgacgg caccgatacc
     5281 ctgatcgcct gcgacaccgg gcccggcaac gcgctgctcg acgatttcat gttccgcacc
     5341 atgggccagc ggttcgattg cgaaggccgc atggcagccc agggcgcggt ggatgtggcg
     5401 tgggtgatgc aagcgctggc gcatccgttc tttgcattgc cgccaccgaa atcgctggac
     5461 cgcaacgatt ttgcctcgca gatcttgcgc ggggtttctc ccgccgacgg tgcggcgacc
     5521 ctgacggcct tcaccgccga agccatcgcc cggatcgctc cgctgttgcc gaagcagccg
     5581 cggagctgga tcgtggccgg cggcggcgcc cgcaatctca cgatgttgcg gttgctgcga
     5641 gagcggctgg agccagccac cgtcgagacc gccgaggggc tgggctggtc cggcgacgcc
     5701 atcgaggcgc aggcctttgg gtttctggcg gcgcggggct tgaagggcct gccgctcagc
     5761 tatcccgcca ccaccggggt cccgatcccg atgaccggcg gggtgatcgc gcggccgtga
     5821 gcggggggcg gtcgatgcaa ttgcatcgag cttgacagtt tcatgccggt gcatgtagtt
     5881 tgatgcaaat gcatctaact acgagacccc ccgaaatggc cgcacccttg aaactgatca
     5941 gccacaagct ttgcccctat gtgcagcgcg cggtgatcgc gctgaacgag aagggcgtcc
     6001 ccttcgagcg gatcgatatc gatctcgcca acaagccgga ttggtttctg gcgatttcgc
     6061 cgctcggtaa gacacccgtg cttcaggtcg gcgacacagc gatcttcgag tcggccgtca
     6121 tcctggaata tctggaggaa acccaaccaa aaccgctgca tccggccgat gccttggccc
     6181 gggccgagca tcgcggctgg atcgaatttg gctcggctgt gctcaacgac atcgccggct
     6241 tttacgccgc agccgatgag gcaaccttca acgccaagac attgcaactc gagcagcggt
     6301 ttgcgcggct cgagacgcgg gtcgcggccg caccatggtt cgacggcgaa agtttttcgc
     6361 tggtcgatgc ggtgttcggt ccggtgtttc gctatttcga cgtgttcgac gacattgccg
     6421 acttcggaat tctggcaggc aagccgaagc taacccaact tgcgcagttg gaggtgtgtt
     6481 tttgtgattt tgcgcgacat gctgcgagtc aaatcaagca gttagacagt gcgtgaaggc
     6541 gatttgggca tgtagtgcca accttggcca ttgacgctcg gaagcgagca tgat
//
LOCUS       DATNOI010000275         4159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2007432,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000275 DATNOI010000000
VERSION     DATNOI010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2007432"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="VN968_10710"
     CDS             <1..171
                     /locus_tag="VN968_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXL29745.1"
                     /translation="RLVESYKPTIVGTVGIGVRKADQELLQKINASLAKLKANGAVDK
                     ILDKWGLKAKAA"
     gene            289..942
                     /locus_tag="VN968_10715"
     CDS             289..942
                     /locus_tag="VN968_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXL29746.1"
                     /translation="MQNFLRDATEFLPILLQGVWLTILVTIGSLMLSTVLGLFWALMR
                     VSGVRALSGLSAGLINVIRGIPIIVLLFYLYFVMPDFGVALTALQASILGLGIAYSAY
                     QAENFRAGIEAIDKGQIEAAQTIGMGWWLTMRRVVLPQAVRIVLPPYGNIMIMLLKDS
                     SQASTITVAELALQGKLIASSTFKNTNVFTLVALMYLTMSIPLILLVRHFEKRANRR"
     gene            939..1670
                     /locus_tag="VN968_10720"
     CDS             939..1670
                     /locus_tag="VN968_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HXL29747.1"
                     /translation="MIKLSNVHKSFGSVEVLKGITASVQKSEVVCIIGPSGSGKSTIL
                     RCINGLESYEAGDIVVEGARVDSHAPSIVAIRTQVSMVFQRFNLFPHRTAIENVVEGP
                     IYVKNEPRAEALERGRTLLAQVGLAEKADAYPAQLSGGQQQRVAIARALAMQPKAILF
                     DEPTSALDPELVGDVLGVMRKLADDGMTMVVVTHEMAFARDVADRVLFIDGGVVVEQG
                     AAKAVLDQPQHVRTQDFLRRVLHPL"
     gene            1693..2988
                     /locus_tag="VN968_10725"
     CDS             1693..2988
                     /locus_tag="VN968_10725"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXL29748.1"
                     /translation="MTVTHTPLALRPSLYADTAVPPVAAPPLETDRTVSVAIIGGGFA
                     GLSTALHLAGHGTDAIVLEAQEPGWGASGNNGGQLNPGLKLDPDTIEATFGTDLGRRM
                     IAFAYDTPVFTLDLIRRHRIACEARQNGTLRAAYHEANAAAVENTAEQCIRRGMPVTV
                     LDRDALREATGTDRYVRAMLDRRGGDLQPLSYARGLAGAAIAAGAAIHGRTPATSLRR
                     EGGRWRIDTPCAVVRADKILIATNGFTDDLWPGLRRTIVPVFSSIAATEPLPDDIARQ
                     IMPTRLVLYESGHITVYYRVDASNRLLMGGRGPMQWISDPSAVSYLTRYAVRLWPALR
                     GIGWTHGWNSRLAMTADHYPHVHEPGPGALVYLGCNGRGVGLATAMGQQLARRLIGGE
                     AAEIDLPITPLKPIRFHALWPVAVKSVVLYGQIRDRLGL"
     gene            3160..>4159
                     /locus_tag="VN968_10730"
     CDS             3160..>4159
                     /locus_tag="VN968_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXL29749.1"
                     /translation="MSETVEFDYVIVGAGSAGCVLANRLSADGKNSVLLLEAGPKDSN
                     LWIHVPLGYGKLFKDNSVNWMYQTEPEPGLGGRQVFQPRGKVLGGSSSINGLLYVRGQ
                     HEDYDRWRQHGNAGWGYEDVLPYFKKAENQQRGADKYHGAGGPLPVSDWRHADPLSEA
                     FVAAAAETGIPANPDFNGATQEGSGLFQTTTRRGRRASTAFSYLRPAKGRGNLHVETS
                     ALAQRILFEGRRARAVEYRQGSSLRTARARNEILVSGGAYNSPQLLQLSGVGPAELLK
                     QHGIGVVLDASGVGNDLQDHMQVRLVTRCAQRVTLNDIVNHPVRRFMAGLQYAALRK"
BASE COUNT          751 a         1296 c         1340 g          772 t
ORIGIN      
        1 cgtctggtcg aatcctacaa accgaccatc gtcggcacgg ttgggatcgg cgtccgcaag
       61 gccgatcagg aactgctcca gaagatcaac gcctcgcttg ccaagctcaa ggcgaatggc
      121 gccgtcgaca agattctcga caaatggggg ctcaaggcaa aagccgcctg acacctgacc
      181 gggcgaagcg gccatcggca ttgcggcgaa atcgagccgg gtcaaaagga tagggcgcga
      241 tcgtgattca atttgaagcg agcagggtgc aacgcgtgat ttgaaccgat gcaaaatttc
      301 ctgcgcgacg ccaccgagtt cctgccgatc ctgctgcagg gcgtatggct gacgatcctc
      361 gtcaccatag gctcgctgat gctgtcgacc gtcctcgggc tgttttgggc gctgatgcgg
      421 gtgtccggcg tgcgggcgct gtccgggctc agcgccggcc tgatcaatgt gatccgcggc
      481 atcccgatca tcgtgctgtt gttctatctc tactttgtga tgcccgactt cggcgtcgcg
      541 ctgaccgcgc tgcaggcttc gatccttggg ctcggcatcg cctattcggc ctaccaggcg
      601 gagaatttcc gcgccggcat cgaggcgatc gacaagggac agattgaggc cgcgcagacc
      661 atcggcatgg gctggtggtt gaccatgcgt cgcgtggtgt tgccgcaggc ggtgcgtatc
      721 gtgctgccgc cctacggcaa catcatgatc atgctcttga aggattcctc tcaggcatcg
      781 accattacgg tcgccgaact ggcgttgcag ggcaagctga tcgcctcctc gacgttcaag
      841 aataccaacg tgttcacgct ggtcgcactc atgtatctca ccatgagcat cccgctcatc
      901 ctgctggttc ggcatttcga gaagcgagcg aaccgccgat gatcaagctc agcaacgtcc
      961 acaagagctt cggttcggtc gaggtgctca agggcattac cgcctcggtc cagaaaagcg
     1021 aggtcgtttg tattatcgga ccgtcgggct cgggcaagtc gactattctg cgctgcatca
     1081 acggcctgga aagttacgag gccggcgaca ttgtcgtgga gggagcgcgg gtcgacagcc
     1141 atgcaccgtc gatcgttgcg atccgaaccc aggtatcgat ggtattccag cgcttcaatc
     1201 tgtttccaca ccgcacggcc atcgagaacg tcgtcgaagg gccgatctat gtgaagaacg
     1261 aaccgcgtgc cgaggcgctt gagcgcggcc gcaccctgct ggcgcaggtc ggcctcgccg
     1321 agaaggccga cgcctatccg gcgcagcttt cgggtggcca gcagcagcgt gtcgccatcg
     1381 cccgcgcgct ggcgatgcag cccaaggcga tcctgtttga cgaaccaact tccgcgctcg
     1441 accctgaact ggtcggcgac gtgcttgggg tgatgcgcaa gcttgccgac gacggcatga
     1501 ccatggtcgt ggtcacgcat gagatggcat ttgccaggga tgtggcggat cgggtgcttt
     1561 tcatcgatgg tggcgtcgtc gtcgagcagg gagcggcgaa ggccgtgctc gaccagccgc
     1621 agcatgtgcg gacgcaggat ttcctgcgcc gcgtgctgca tcctttgtga cgcggagccg
     1681 ccggatgttg cgatgacggt gacccacact ccgcttgcgc tgcgaccaag cctttatgcc
     1741 gataccgccg ttccgccggt tgcggcgcct ccgctcgaga ccgacaggac cgtgtcggtc
     1801 gcgatcatcg gtggcggctt tgccggactt tcgaccgcgt tgcatctcgc cgggcatggg
     1861 accgacgcga tcgtcctcga agcccaggag ccgggctggg gcgcgtcggg caacaacggc
     1921 ggtcaactca acccggggct caagctcgat cctgacacca tcgaagcgac cttcggcacc
     1981 gatctcggcc ggcgcatgat cgcgtttgcc tacgacacgc cggtcttcac gctcgatctt
     2041 atccgccgtc atcggatcgc ctgcgaggcg cggcagaacg gcacgctgcg cgccgcctat
     2101 cacgaagcga acgcggccgc cgtcgagaac accgcggaac agtgcatccg gcgcggcatg
     2161 ccggtcacgg tactcgaccg ggacgccctg cgagaggcga cgggaacgga tcgatacgtc
     2221 cgcgccatgc tggatcggcg cggtggcgat ctgcaaccgc tgagctatgc gcgcggtctt
     2281 gcgggtgccg cgattgcagc cggcgccgcg attcacggcc ggacgcccgc gacgtcgttg
     2341 cggcgcgaag gcgggcgatg gcgcatcgat acaccgtgcg cggtggttcg ggccgacaag
     2401 atcctgatcg ccaccaacgg tttcaccgac gatctctggc ctggcctgcg ccgcacgatt
     2461 gtgccggtgt tcagctcgat cgcggcaacc gagccgctgc ccgacgatat cgcgcgacag
     2521 atcatgccga cgcgattggt gctgtatgaa agcggccata tcaccgtcta ctatcgtgtc
     2581 gatgcatcga accgcctgct gatgggcggt cgcggtccga tgcaatggat cagcgatcca
     2641 tccgcggtca gctatctcac gcgctatgcg gtccggcttt ggccggcgct tcgtggtatc
     2701 ggctggacac acggctggaa cagccgcctc gccatgaccg ccgaccacta cccacatgtg
     2761 catgagcctg ggcccggcgc gctggtctat ctgggctgca acggccgtgg cgtcggcctc
     2821 gcaaccgcaa tgggacaaca actggcgcgc aggctgatcg gcggcgaggc cgccgagatc
     2881 gacttgccga ttacacccct caagccaatc cgcttccacg cattatggcc ggtggcggtg
     2941 aagagcgtcg tgctctatgg acaaattcgc gatcgcctcg gactgtaggg ctttgatacc
     3001 tgacggtgac ggcgaaaaag gtcgccctgc gatctcgccg cgtgcattga cagacctgaa
     3061 taagacattc agatagcaag gaattcgcgc atgtctgcga tttcaacctg tcctcgacaa
     3121 ggtctggata actagcgcga tacacggggt gggggcgaat tgtccgaaac tgtcgaattc
     3181 gactatgtca tcgtcggcgc cgggtcggcc gggtgcgtgc tcgccaatcg cctcagcgcc
     3241 gatggaaaga attcggtgct gctgctcgaa gccggcccga aggactctaa tctctggatt
     3301 cacgtgccgc tcgggtatgg caagctgttc aaggacaaca gcgtcaactg gatgtaccag
     3361 accgaaccgg agccgggact gggcgggcgt caggtgtttc agccgcgcgg caaggtgctc
     3421 ggcggctcga gttcgatcaa cggcctgctc tacgttcgcg gacagcatga ggattacgat
     3481 cgctggcgcc agcacggcaa cgccggctgg ggctatgaag acgtattgcc gtatttcaag
     3541 aaagccgaga accagcaacg cggggccgac aagtatcacg gcgccggcgg cccgctgccg
     3601 gtgtcggact ggcggcacgc tgatccgctg tcggaagcct ttgtcgccgc cgcggcggag
     3661 accggcatcc ccgcaaatcc cgacttcaac ggcgcgacgc aggaaggctc gggcttgttc
     3721 cagaccacga cgcggcgcgg cagacgggcg agtacggcgt tttcctatct tcgaccggcc
     3781 aaagggcgcg gcaatctgca tgtcgagact tccgcgctgg cgcagcgcat cctgttcgaa
     3841 ggtcgtcgcg cccgggccgt tgagtaccgg cagggcagca gcctgcgaac ggcgcgggca
     3901 aggaacgaga ttctggtctc cggcggcgcg tataattcgc cgcaattgct ccagctctcc
     3961 ggcgtcgggc ctgcggagct tttgaagcag cacggcatcg gcgttgtgct cgacgcatcc
     4021 ggcgtcggca atgacctgca ggatcacatg caggtcaggc tggtgacgcg ttgcgcccag
     4081 cgcgtcaccc tcaatgatat cgtcaatcat ccggttcgcc ggttcatggc cggtctgcaa
     4141 tacgccgcac ttcgcaagg
//
LOCUS       DATNOI010000276         4203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_385027,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000276 DATNOI010000000
VERSION     DATNOI010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385027"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1432
                     /locus_tag="VN968_10735"
     CDS             95..1432
                     /locus_tag="VN968_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HXL29750.1"
                     /translation="MADEQLFEDLPEQERPQAEVRGALRLRVPERSQIGMQVAALDDL
                     ISEDHPVRSVWAFVEGLDLSALHEVIKAREGQPGHPPAAPELMVALWLWATVDGVGGA
                     RQLDRLCRDHLVYRWLCGGVSMNYHSLSTFRTAHMAVLERLLAQGVASMVQAGLVSLD
                     TLAQDGLRVRAGAGAGSFRRHARLDDLLTAAQTRVERLRAEVETDPAAGDRGRRAAKE
                     RGAREREARIAAAQERMKELEAERERREKTNKKEVAKQKPPRASTTDADARVMKMADG
                     GWRPAYNMQIISTPEHQVIVAVDIDTSGSDRGLAQPGIETVHAEGYEPSNYLVDGGFT
                     KNDAIEWAHANDIKVWCPPAQTKHGTDPYAPKKGDGVGVADWRERMKSEDGKAFYRLR
                     CEHECINAHARRMGLRQLTLRGKIKARIHLLWFAVAHNMMRFFALHAKAQAAA"
     gene            complement(1724..2410)
                     /locus_tag="VN968_10740"
     CDS             complement(1724..2410)
                     /locus_tag="VN968_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29751.1"
                     /translation="MPIITEIQETVLELDRLQRQLDRVNTSLALFDRPARFLFLNGGI
                     YVLLPSLLLLAAHYLVTVAMNVSPLYLRIASVIIPMPFGMAAFALSKIGYRGAFGLGA
                     LTAAVSVTGMFAVVAYLDGTSMVPETLREWRETFEYGLSIALAFLTGNILVILVLFIL
                     PSTIASSGKPNAAAYRMARMLGQHVGQETMRRRARRIQDLMQTVGPLLGVVATAAGSI
                     YTGLKGVFGH"
     gene            complement(2698..3096)
                     /locus_tag="VN968_10745"
     CDS             complement(2698..3096)
                     /locus_tag="VN968_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29752.1"
                     /translation="MPIPARAAPLITAEEAALPPQKGAVPNSGRGITRGPKIQVPEAE
                     AGVQTSPMRFQVKFQTFGGSSIDLDALKVTYLKSPVVDLTPRIKPFALPTGIDMPDAL
                     LPPGDHLLRIDIKDSDGRVSSISFLLKIAP"
     gene            complement(3204..>4203)
                     /locus_tag="VN968_10750"
     CDS             complement(3204..>4203)
                     /locus_tag="VN968_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXL29753.1"
                     /translation="TNGALGLLPLSLLPTAAAEVKTDDEPLFSSYRNVPWLARTHAVT
                     MVPSASALQTLRKLPPGKASRQDLVAFGDPYFSKEQADEAAKGDKPVQLADASGTTRG
                     APLKRRSSPQLEGADNADLAMLPRLPDTADELKSIALALQADPSKVLNLGKDANESAV
                     KKMDLSGFKIVAFATHGLVPGELDGLTQPALALSAPSLSGSEGDGLLTMEEILSLKLD
                     ADWVVLSACNTGAGAGAGAEAASGLGRAFFYAGTRALLVTNWSVHSQSAKDLVTDLFK
                     RQADDPKLTRGEALRQAMMALADGKGYTDASGKTEFAYAHPLFWAPYTIIGDGGRR"
BASE COUNT          818 a         1272 c         1351 g          762 t
ORIGIN      
        1 gctcctcaga gtgtgagaat ctttcggtaa cgagcgtttg ccacggccgc catgctgtga
       61 ttcgctggca ttgagcgcga agcaggggct ggggatggct gacgagcaat tgttcgagga
      121 tttgcccgag caggagagac cgcaggcgga ggtgcgcggt gcgctgcggc tgcgggtgcc
      181 agaacgcagt cagatcggta tgcaggtggc agcattggac gatctgattt cggaggatca
      241 tccggtacgc tcggtttggg cgttcgtcga ggggctggat ctgtcggcgt tgcacgaggt
      301 catcaaggcg cgcgagggcc agcccggaca tccgccagca gcgccggagc tgatggtggc
      361 gctttggcta tgggccacgg tcgacggggt cggcggcgcc cgccaattgg accggctgtg
      421 ccgcgatcat ctggtctatc gctggttatg cggcggggtg tcgatgaact atcacagcct
      481 gtcgaccttc cggaccgccc atatggcagt tctggagcgg ctactggcgc agggcgtcgc
      541 gtcgatggtg caggctgggt tggtgtcgct cgacacgttg gcgcaggatg gcctgcgggt
      601 acgcgcgggt gccggggcgg gttcgttccg ccggcacgcg cggcttgatg atctgctcac
      661 cgcggcgcag acgcgggtgg aacgcctgcg cgctgaagtc gagacggatc cggcggctgg
      721 cgatcgtggc cggcgcgcgg caaaggagcg tggcgcccgc gagcgcgagg cgcggatcgc
      781 ggcggcgcag gagcgcatga aggaactgga ggccgagcgg gaacggcgag agaagaccaa
      841 caagaaagag gtggccaagc agaagccgcc gcgcgcttcg accaccgatg ccgacgcgcg
      901 ggtgatgaag atggcggatg gtggatggcg ccccgcttac aacatgcaga tcatatcgac
      961 gccggaacac caggtgatcg tcgcggtcga catcgatacc agcggttcgg atcgcggcct
     1021 ggcgcaaccg gggatcgaga ccgtgcacgc tgaaggctac gaaccctcca attacctggt
     1081 cgatggcggc ttcaccaaaa acgacgctat tgagtgggcc catgccaacg atataaaggt
     1141 atggtgcccg ccagcgcaga ccaaacacgg caccgaccct tatgctccga agaaaggtga
     1201 cggtgttggc gtcgcggact ggcgtgaacg catgaagagc gaggacggca aggcgttcta
     1261 ccgactgcgc tgcgaacacg aatgcatcaa cgcccatgcc cgccgcatgg gcctgcgcca
     1321 gctaaccctg cgcggcaaga tcaaggcccg catccatctg ctgtggttcg cggtggctca
     1381 taacatgatg cgcttcttcg cgttgcacgc caaggcgcag gcagcggcat agcagggagc
     1441 ccacgctgac agcacctacc aagcgccctc gcctccggtc ggctcagcca agcatcgggg
     1501 taacccagct tccgcaatgc ggaattgcgc ttgatcgccg ccattcggtc gccgactcgg
     1561 ccgaaaactc cagcagccgt aaaattttgt gggcgagcgc gaaacattct caccctctca
     1621 gcatgaggtt gttgtgtttc aacgagttag acctcatcct gaggaggtca cgaagtggcc
     1681 gtctcgaagg atgggctgca ataccgattt gcgattccag gtactaatga ccgaatacgc
     1741 ccttcagccc ggtatagatc gagccagcgg cggtcgcgac cacgccgagc agcggcccca
     1801 cggtctgcat caggtcttga atacgccgcg cccgtcgccg catggtttcc tgcccgacat
     1861 gctgtcccag cattcgggcc attcggtagg cagcagcgtt cggcttcccg ctcgacgcaa
     1921 tcgtcgaggg aaggatgaag agtacgagta tcaccagaat atttccggta aggaaggcaa
     1981 gcgcgatgct caagccgtac tcaaatgttt cccgccactc ccgcagggtt tcgggcacca
     2041 tcgaggttcc atcgagatag gcgactacgg cgaacatgcc ggtcacgctg acggccgcgg
     2101 tcaaggcacc gaggccgaac gcaccccgat atccgatctt gctcagcgcg aacgccgcca
     2161 ttccgaacgg catgggaatg atcaccgagg cgatccgcaa atagagcggc gatacattca
     2221 tcgcgaccgt caccaggtag tgagctgcga gcagcagcag cgacggcagc agaacgtata
     2281 tgcctccatt cagaaacaga aatcgcgccg gacgatcgaa caatgccagc gaggtgttga
     2341 cccggtcgag ttgccgctgc aggcggtcga gttcgaggac ggtctcctga atctccgtga
     2401 tgatcggcaa ggcaactctc agatcgcggt tgcaatgctt gcaaacaacg gcctcgtcct
     2461 tgatcgtctc tgcgcaaaaa ggacattcca tcggacgcta agccggttta cgcctgcggc
     2521 gtgccgcaag ccgtgcattt gcctccgcaa gttcggaccg cagtcgatcg cgcttcgtca
     2581 acagttcggc gcgttcggtg atgagcgact ccggaatcgc ggtgtcacga ccgcatgtcg
     2641 cgcagaccgg agccagatcg tccttttcct cgttgcagaa cgggcatcgc atcggcgcta
     2701 aggcgctatc ttcagcagga aacttatgct cgacacacgg ccatccgaat ccttgatatc
     2761 gatccgcagc agatgatcgc caggcggcaa tagcgcatcc ggcatatcga tcccggtggg
     2821 cagtgcgaaa ggcttgatgc gtggtgtcag atccacgacc ggcgatttga ggtaggtgac
     2881 cttcaatgca tcgaggtcga tactcgaccc gccgaaagtc tgaaacttga cctgaaaacg
     2941 catcggcgaa gtttgcacgc cggcctccgc ctcgggcact tgaattttgg ggccgcgtgt
     3001 aatgccacgt cccgaattcg gaaccgcgcc tttttgcggc ggcagcgcgg cttcctccgc
     3061 tgtgatcagc ggcgccgcgc gagctggaat cggcattgcc gtcgccgcgc cgaaaattgc
     3121 cgcgcaggtc agtgtcaaaa gatgcctgaa catgattctc cattcattgc agtatggacg
     3181 ttaccggaca tcaccgattt gcgtcaacgt cgtccgccgt cgccgatgat cgtgtagggc
     3241 gcccagaaca gtggatgggc gtaggcgaat tcggtcttgc cggaggcgtc ggtgtaacct
     3301 ttgccgtcgg caagcgccat catcgcctgc cgcagcgcct cgccgcgcgt cagctttgga
     3361 tcatccgcct gccgcttgaa caggtcggtc acgagatcct tcgccgattg cgaatggacc
     3421 gaccagttgg tgaccagcaa tgcgcgggtg cccgcataga agaacgcccg gccgagaccc
     3481 gaggcggctt cggcgccggc gcccgcgccg gcaccggtat tgcaggccga cagcacgacc
     3541 caatcggcat cgagcttgag cgagagaatc tcttccatgg tcaggagccc gtcgccttcg
     3601 gatcccgaca gcgagggcgc cgatagagcg agagctggtt gggtcaatcc atcgagttcg
     3661 ccgggcacca ggccatgggt cgcgaatgcg acgatcttga accccgacag atccatcttc
     3721 ttgacggcgc tctcgttggc gtccttgccg agattgagca ccttcgacgg gtccgcttgc
     3781 agcgccagcg caatcgattt cagttcgtcg gccgtgtccg gcagccgcgg caacatcgcg
     3841 aggtcggcgt tgtcagcccc ttcgagttgc ggactgctgc gccgcttcaa cggcgcgccg
     3901 cgcgtggtcc cggatgcatc agccaactga accggcttgt cgccctttgc cgcctcatcg
     3961 gcctgctctt tgctgaagta aggatcgccg aaggcaacca gatcctgccg tgatgccttg
     4021 ccgggcggta atttgcgcaa ggtttgcaac gccgacgctg acggcaccat tgtcacggcg
     4081 tgggtgcggg caagccacgg cacattgcgg tagcttgaaa acagcggttc atcatcggtc
     4141 ttgacttcag ccgccgcggt cggcagcagc gacaacggca gaagacccag tgcgccattg
     4201 gtc
//
LOCUS       DATNOI010000277         4589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2551523,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000277 DATNOI010000000
VERSION     DATNOI010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2551523"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VN968_10755"
     CDS             complement(<1..359)
                     /locus_tag="VN968_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL29754.1"
                     /translation="MITGLDHIVVLLGDIKAGAKACELVLGRAPSWRSQSDGAETVLF
                     TLDNMTLELMAPAGSTPTADRIRGVAKIRGEGLASICFRVRDIAKMHRRLDRLALKPE
                     PVAEVESRDTDSGGTLH"
     gene            complement(418..1677)
                     /locus_tag="VN968_10760"
     CDS             complement(418..1677)
                     /locus_tag="VN968_10760"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HXL29755.1"
                     /translation="MTVYSGPVFDMAVNQFGVIANHLSIPMDERDRLLMPKRAITISC
                     PIHRDDGTIAVFEGYRVQHHLTLGPTKGGTRFAPSVDIGEVAALAIWMSWKCALVGLP
                     YGGAKGGVNVDLATISKRELEALSRRYMQEMIPFVGPHTDVMAPDMGTNEQVMAWFMD
                     TYSMYQGRTVTEIVTGKPVSSGGTLGRLEATGRGVAHLAKRVMKELAIDLNNGTAVIQ
                     GFGNVGSYAALELQRFGLKVIAVSDHTGALYDETGLDIPALMRHSGVHGSIAAFSNEL
                     TFDPAQILTLPCDVLVPAAMERVIDAHIAENLKCRVLAEGANGPTTPDADLVLEKRQD
                     EIFLIPDILCNSGGVVVSYFEWVQDLQQLFWEEEEVMRREYQILDRAFDQMAARAKAD
                     QIPHRTAAMAIGVEKVRAAKTTRGLFP"
     gene            1898..2809
                     /locus_tag="VN968_10765"
     CDS             1898..2809
                     /locus_tag="VN968_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HXL29756.1"
                     /translation="MQALFIGQTYIDVTFITDHMPTGDEKHVASAYAVSFGGNAVTAA
                     FCCAKLGIVPDLIATVANDWLGRMFQDMSAKYGISIHPRKVNSSSLSFIMPKDGKRAI
                     VRCRDDEHIHPFPLLNLGGCRALHIDGHQPDAAIHYAKLCREDGILTSLDGGGLRTNT
                     HELLEFIDVAIVAERLCEQMDLTPEKMLDYLKSRGCRVGGVTQGERGLLWYDEAGAVR
                     TMPALPISRERVLDTNGAGDVFHGAYVFSYLNNPGKSWHDHFEFARAASTYKIQRLGN
                     EAGLPTLADIENVKQEFQIGRKKEFWD"
     gene            2813..3754
                     /gene="rbsK"
                     /locus_tag="VN968_10770"
     CDS             2813..3754
                     /gene="rbsK"
                     /locus_tag="VN968_10770"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HXL29757.1"
                     /translation="MGRVFVAGSINMDVVATADRHPRIGETIAGQAVLYFPGGKGANQ
                     AVAAAKLGAATTLIGRLGRDGFGDELKAFLAAQGIDLSFVQPTSDTHTGTAIITIANA
                     DNTIVVIPGANAHVSAADVEAPMLAKADIAVSQFEIPLPTITAFFKRARAAGATTILN
                     PAPAVEFGRELLDLVDILILNESELGLLAKTELHDTDDHARFVDAARSLQAGQDKIIC
                     VTLGKRGVLALVNDQPEIIPGRAVTAVDTTGAGDCFVGAVAALLAGGQPIRNALDYAN
                     AAASICVQRMGAAPSMPTAAEVEATLSSGRPGERRDP"
     gene            complement(3861..>4589)
                     /locus_tag="VN968_10775"
     CDS             complement(3861..>4589)
                     /locus_tag="VN968_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HXL29758.1"
                     /translation="AKGLEVDVIELGARVMARAVTAEISDYFQERHTAAGIRIHLGVQ
                     ATSIESDGTNVTGVSLSDGHHVPADLVVVGVGVLPNVELAAEAGLPVAAGIIVDEQLL
                     TSDPNISAIGDCALFASPRFGGSLRLESVQNATDHARCVAARLTGDARTYDGQPWFWS
                     DQGDYKLQIAGLTTGYDRVVVRGDRGQRSFSAFCYKAGQLVGVESINRGSDHVFGRKI
                     LGMNRSIEPEQAADMSFDLKAALA"
BASE COUNT          847 a         1497 c         1392 g          853 t
ORIGIN      
        1 cagtgcaggg tcccgcccga atcggtgtcg cggctttcga cctcggccac cggctccggc
       61 ttcagcgcca ggcgatcgag cctgcggtgc atttttgcga tgtcgcggac gcggaagcaa
      121 atgctggcga gcccctcgcc ccggattttc gccacgccgc ggattcggtc ggcggtcggt
      181 gtgctgccgg ccggcgccat cagctcgagc gtcatgttgt cgagcgtgaa cagcacggtc
      241 tcggcgccgt cgctctggct tcgccacgac ggcgcgcgcc cgagcaccaa ttcgcaagcc
      301 ttggcgccgg ccttgatatc ccccagcagc acgacgatgt ggtccaggcc ggttatcatc
      361 gaggcaatcc cttgatcgag aaatggcgct ctggcttcac gagcgccgcc tcgatagtta
      421 tggaaacagc cctcgcgtgg tcttcgcggc gcggaccttt tccacgccga tcgccatcgc
      481 ggcggtgcga tgcggaatct ggtcggcctt cgcgcgggcc gccatttggt cgaaggcgcg
      541 atcgaggatc tggtattcgc gccgcatcac ttcctcttct tcccagaaca gctgctgcag
      601 atcctgcacc cactcgaaat agctcaccac gacgccgccg gaattgcaga ggatgtcggg
      661 tatcaggaaa atttcatcct ggcgtttttc caacaccaga tcggcgtccg gcgtggtcgg
      721 tccgttggcg ccttccgcca gcacgcgaca tttgaggttt tctgcgatat gcgcgtcgat
      781 cacgcgctcc atcgccgccg gcaccagcac gtcgcagggc agtgtgagaa tttgcgcagg
      841 atcgaacgta agctcgttgg agaaagcggc gatactgccg tgcacgccgg aatgtcgcat
      901 caacgcgggg atatcgagac cggtctcgtc gtaaagcgcg ccggtgtgat cgctgaccgc
      961 gatcaccttg aggccgaacc gctgcaattc gagcgcggca taggagccga cattgccaaa
     1021 accctggatc accgcggtgc cattgttcag gtcgatcgcg agctctttca tcacccgctt
     1081 cgcgagatgc gcgaccccgc gccctgtcgc ttcgagccgg cccaatgtgc cgccggagga
     1141 cactggcttg ccggtgacga tctcggtgac ggtgcggccc tgatacatgg aataggtgtc
     1201 catgaaccag gccatcacct gctcattggt gcccatgtcc ggcgccatca cgtcggtatg
     1261 cggcccgacg aacggaatca tttcctgcat gtagcgccgc gacagcgcct ccagctcgcg
     1321 tttggaaatc gtggccagat cgacgttgac cccgcccttg gcgccgccat aggggaggcc
     1381 gaccagcgcg cacttccagc tcatccagat cgcaagtgcc gccacctcgc cgatatcgac
     1441 actgggcgcg aaacgcgtgc cgcccttggt cggaccgagg gtaagatgat gctgtacgcg
     1501 atagccctcg aacaccgcga ttgtgccgtc gtcgcgatgg atcggacagg agatggtgat
     1561 cgcccgtttc ggcatcaaca gacggtcgcg ctcgtccatc gggatcgaca ggtgattggc
     1621 gatgacgccg aactgattca ccgccatatc gaataccggg ccggaataga ccgtcatcgt
     1681 cgctcctttt ttctctctgt gaggaactct gaaccgccgc tggccgtcgg aaatgcgacg
     1741 cactggcgat gtggaaccac ggtcgtagtc ccgccataca cttatctcaa gcgattaagc
     1801 gggtgattcg gctaataatt ttagcaaatc tccggcgcga agcggtgcta gtgtggggtg
     1861 ccgccgggac caccatcagc gtatgacgga tgactttatg caggccctct tcatcggaca
     1921 aacctatatc gacgtcacct tcattaccga ccacatgccg actggcgacg agaagcatgt
     1981 ggcgtcggcc tatgcggtct cgttcggcgg caacgccgtc acggcggcct tctgctgtgc
     2041 caagctcggc atcgtgccgg acctgatcgc caccgtggcc aacgactggt tggggcggat
     2101 gtttcaggac atgagcgcga aatacggaat ctcgatccac ccgcgcaagg tcaattcctc
     2161 ctcgctgtct ttcatcatgc cgaaggatgg caagcgggcg atcgtgcgct gtcgcgacga
     2221 cgagcatatc caccccttcc cgcttttgaa cctcggcggc tgccgcgcac tgcatatcga
     2281 cggccaccag ccggacgccg cgatccacta tgccaagctg tgccgcgaag acggcatcct
     2341 gacatcactc gatggcggcg gtcttcgcac caacacccac gaactgctgg aattcatcga
     2401 cgtcgccatc gtcgccgaac ggctgtgcga gcagatggac ctgacgccgg aaaagatgct
     2461 ggattatctc aagagccggg gctgccgggt cggcggcgtg acccagggcg aaagaggcct
     2521 gctctggtac gacgaagcgg gggcggttcg taccatgccc gcgctgccga tttcgcgcga
     2581 gcgcgtgctc gataccaacg gtgccggtga tgtatttcac ggcgcttacg tgttttcgta
     2641 tctcaacaat cccggcaaaa gctggcacga ccatttcgaa tttgcgcgcg cggcctcgac
     2701 atacaagatc caacgcctcg gcaacgaggc cggattgccg acgcttgccg acatcgaaaa
     2761 cgtcaagcaa gagtttcaga tcgggcgtaa gaaggaattt tgggattagg caatgggccg
     2821 cgttttcgtt gccggaagca tcaacatgga tgtggtggcg acggccgacc ggcatccgcg
     2881 catcggcgag acgatcgccg gccaggccgt gctttatttt cccggtggca aaggcgccaa
     2941 ccaggcggtc gcggcggcga agctgggtgc agcgacgacg ctgatcggcc ggctcggcag
     3001 ggatgggttc ggcgatgaat tgaaggcgtt cctcgccgcc cagggaatag acctgagctt
     3061 cgtgcagccg acttccgata cccataccgg aacggccatc attaccatcg ccaatgccga
     3121 caacaccatt gtcgtcattc ccggcgccaa tgcgcatgtg agtgccgccg atgttgaggc
     3181 gcccatgctc gcgaaggccg atatcgcggt cagccagttc gaaattccat tgcccaccat
     3241 caccgccttc ttcaagcgcg cccgcgcggc gggtgccacc acgatcctca atccggcgcc
     3301 cgcggtcgaa ttcggccgcg agctcctgga tctcgtcgat attctcatcc tcaacgagag
     3361 cgaactcggc ctgttggcga agaccgagct gcacgacacc gacgatcacg cgcgcttcgt
     3421 cgacgcggcg cgatcgctgc aagccggtca ggataaaatc atctgcgtga ccctgggaaa
     3481 gcgcggcgtg ctggcgctcg tcaacgacca gcccgagatc attccgggcc gcgccgtcac
     3541 ggccgtcgat actaccggtg ccggcgattg ttttgtcggc gccgtcgcag cgctgctcgc
     3601 cggcggacag ccaatccgca acgcgctgga ctacgccaat gccgcggcct cgatctgcgt
     3661 gcagcggatg ggcgccgcgc catcgatgcc gacggcagcg gaagttgagg caaccttatc
     3721 ttcaggtcgt cccggcgaac gccgggaccc ataaccactg accccgatga ttgcagaggg
     3781 tatctgccac acccctaaac gagacaacac ggcgtatagg ttccggcgtt cgccggaacg
     3841 acgaattcag cagtatcgac tcacgctagc gcagccttca aatcgaagct catgtccgcc
     3901 gcctgttccg gctcgatcga ccggttcatg cccaggattt tccgtccgaa cacgtggtcc
     3961 gacccgcggt tgatcgactc gacgccgacg agctgcccgg ccttgtagca aaatgccgag
     4021 aacgatcgct gcccgcgatc gccgcgcacc acgacacgat cgtatccggt ggttagcccc
     4081 gcgatctgca gcttgtagtc gccctggtcg ctccagaacc acggctggcc gtcataggtc
     4141 ctggcatcgc cggtcagccg cgccgcgacg caacgcgcgt gatcggtggc gttctgcacc
     4201 gactccagcc gcagcgaccc gccgaagcgc gggcttgcga acagcgcgca gtcgccgatc
     4261 gcggagatgt tcggatccga cgtcagcaat tgttcgtcga cgatgatccc cgccgcgacc
     4321 ggcaggccgg cttctgctgc cagttcgaca ttgggcagaa ccccgacgcc gaccacgaca
     4381 aggtccgctg gcacatggtg gccgtcgctg aggctgacgc cggtcacgtt ggtgccatcg
     4441 ctctcgatgc tggtggcctg cacgccgaga tggatgcgaa tgcccgccgc ggtatggcgc
     4501 tcctgaaagt agtccgagat ttccgcggtc accgcgcgcg ccatgacgcg ggctccgagc
     4561 tcgatcacat ccacctcgag gcccttggc
//
LOCUS       DATNOI010000278         2132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1100266,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000278 DATNOI010000000
VERSION     DATNOI010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100266"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="VN968_10780"
     CDS             complement(<1..1632)
                     /locus_tag="VN968_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXL29759.1"
                     /translation="MVTAPRQIPDAVRATQARASDPKSSAFVSANAGSGKTHVLVQRV
                     IRLLLNGVPPEKILCITFTKAAAANMAERVFSTLGHWVTLDDNALDAAIVDAGITRPD
                     AILRMRARELFACALETPGGLKVQTIHALCTRLLQQFPFEANVPARFSVIDDRDQNEM
                     MERANLKVLLDASRDPNSAAGRALNVAMASAADVTFKDVVREACLSRDHFMAWTDSAG
                     SADAAAAQISTALDVDPADRIEEVEREIIDGPNLPRSRWKEVAATLDAGSKSEQGHAA
                     RLRQALISIGSAQVEEYLGIFLTDERTPRKTVMTKKFGDSHPAIARLFELEGDRIGPL
                     IERRRAVTARDRTQALLQIATAAAANYRREKQERGLLDYDDLIDKTLAMLDRVSSGWV
                     HYKLDRGVDHVLIDEAQDTSPRQWDIVAHIISEFTSGQGARDGVTRTIFAVGDEKQSI
                     FSFQGAAPREFDTRRRGLEKKFIDAGLKFDPVSFTYSFRSGPAILQSVDYVFREEAIY
                     RSIHSAETGYPIHNFLADAGPSLIDLWELQLPDKKE"
     gene            complement(1626..>2132)
                     /locus_tag="VN968_10785"
     CDS             complement(1626..>2132)
                     /locus_tag="VN968_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXL29760.1"
                     /translation="GEIPIRLDNERTFVLSARADRIERRQDGTFAILDYKTGQPPTGK
                     QVRMGLSPQLTLEAAILREGGFEGIPADSLVGELVYVRLSGNNPPGEQKSLELKFNKS
                     DAPQPPDVAADEARLKLEALIRAFENETQAYTSLNLSMWSNRYGNYDDLARIKEWSAA
                     GGLGIEEW"
BASE COUNT          360 a          662 c          693 g          417 t
ORIGIN      
        1 ctccttcttg tccggcagct gcagttccca gaggtcgatc aggctcggtc cggcatcggc
       61 gaggaagttg tggatcgggt acccggtttc ggcggaatgg atgctgcggt agatcgcctc
      121 ttcccgaaac acataatcga ccgattgcag gatcgcaggg cccgaacgga acgagtaggt
      181 gaacgatacc gggtcgaatt tcaggccggc atcgataaac tttttttcca ggcctctgcg
      241 gcgggtgtcg aattcgcgcg gcgccgcgcc ctgaaatgag aagatcgact gcttctcgtc
      301 gccgaccgcg aagatggtgc gggtaacgcc atcgcgggcc ccttggcctg acgtgaattc
      361 ggaaatgatg tgggcgacga tgtcccattg ccgggggctg gtgtcctgcg cctcgtcgat
      421 cagcacgtga tcgacgccgc gatcgagctt gtaatgcacc cagccggagg agacgcggtc
      481 cagcatcgca agtgtcttgt cgatcaggtc gtcatagtcg agcaggccgc gctcctgctt
      541 ctcgcgccgg tagttcgccg ccgccgcggt tgcgatctgc aacagcgcct gggtccggtc
      601 gcgggccgtg accgcacggc ggcgctcgat cagcggtccg atacgatcgc cttccaactc
      661 gaacagacgc gcaatcgccg gatggctgtc accgaatttt ttcgtcatca cggttttacg
      721 gggcgtgcgt tcgtcggtga gaaatatccc gagatactcc tccacttggg cgctgccgat
      781 cgatatcagt gcttgccgaa gccgcgcggc gtggccttgc tcggacttgc tgccggcgtc
      841 gagggttgcc gcgacttcct tccagcgcga tcttggcaag ttgggcccgt cgatgatctc
      901 gcgctcgacc tcttcgatgc ggtcggcggg atcgacatcg agggcggtgg agatctgcgc
      961 cgcggcggcg tcggcgctgc cggcgctgtc ggtccatgcc atgaagtgat cgcggctcag
     1021 gcaggcctcg cgcacgacat ccttgaaggt gacgtcggca gcgctggcca tggcgacatt
     1081 gagcgcccgg ccggccgcgc tattgggatc gcgagaggcg tcgagcagca ccttgagatt
     1141 ggcgcgctcc atcatctcgt tctggtcgcg gtcgtcgatc acggaaaaac gcgccgggac
     1201 gttggcctcg aacgggaatt gctgcagcag ccgggtgcac agcgcgtgga tggtctgcac
     1261 tttcaatcca cccggcgttt ccagcgcgca ggcgaacaat tcgcgggcgc gcatccttaa
     1321 aatcgcatcg gggcgcgtaa ttcccgcatc aacgatcgcg gcatcgagcg cattgtcgtc
     1381 gagcgtgacc caatggccga gggtggagaa cacccgctcg gccatattgg ccgcggcggc
     1441 cttggtgaag gtgatgcaga ggatcttctc cggcggcacg ccattgagca acagccggat
     1501 cacgcgctgc accagcacgt gggtcttgcc cgacccggca ttggcggaga cgaacgcgga
     1561 agacttagga tccgatgcgc gcgcctgcgt ggcgcggacg gcgtcgggaa tctggcgcgg
     1621 cgcggtcacc attcttcgat tcccagtccg ccggccgcgg accattcctt gatccgggcg
     1681 agatcgtcgt aattgccgta gcggttcgac cacatcgaca gattaagcga ggtataggcc
     1741 tgggtttcat tctcgaaggc gcggatcagc gcctccagct tcagtcgcgc ctcatcggcg
     1801 gcgacatcgg gcggctgcgg ggcatcgctt ttgttgaact tcagctccag tgatttctgc
     1861 tcgcccggcg gattgttgcc gctcaggcgc acatagacca gttcgccgac caagctatca
     1921 gccggaatac cttcaaaacc gccttcgcgc aggatcgccg cctccagcgt cagctgcggc
     1981 gacagcccca tccgcacctg cttgccggtc ggcggttgcc cggtcttgta gtcgagaatg
     2041 gcaaacgtcc cgtcctggcg gcgctcgatc cggtcggcgc gtgccgacaa aacaaaagtg
     2101 cgctcgttgt caagccggat cggaatttcg cc
//
LOCUS       DATNOI010000279        20170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_580169,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000279 DATNOI010000000
VERSION     DATNOI010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 20170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20170
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580169"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="VN968_10790"
     CDS             complement(<1..1222)
                     /locus_tag="VN968_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXL29761.1"
                     /translation="MNIQDNSRYHQAHVRSLVDPEGFWGEAAREIDWIEPAKKVFDPA
                     SGLYGRWFAGAVVNTCYNALDRHVANGRAEQVALIHDSPQAGRVTRLTYAQMLHEVKT
                     LAAIMGDFGVAKGDRVILYMPMVPEAMIAMLACARIGAVHSVVFGGFAAKELATRIED
                     AKPKLIFSASCGLEPGRIVQYKPLLDEAIKLSSDKPQACIILQRPQWACELVGGRDHD
                     WSELRTKAMSAKKSAACVPVLATDPLYILYTSGTTGKPKGVVRDNGGHLVALKWSMFN
                     LYGVNPGEVWWCGSDIGWVVGHSYIIYGPLIHGATSIMYEGKPIGTPDAGAFWRVIAE
                     HKAVALFTAPTAFRAIRKEDPDGQFIRQYDLSKFRTLFLAGERADPPTVHWAEKQLKV
                     PVIDHWWQTETGWCI"
     gene            complement(1337..1861)
                     /locus_tag="VN968_10795"
     CDS             complement(1337..1861)
                     /locus_tag="VN968_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29762.1"
                     /translation="MRDGLSGLIRSVALASVLWATPAYHACATGNEPVTDPQIDPAPC
                     VAAAAADDADKIIAVCGALVDNDKTLRADRIKALIARAGAYGRKDMIDRAIGDYDTAL
                     RLDPALSDIFNARGELWRKKGDRPRALADFGAAIKLNPQHEAARANYRSLALELERIG
                     AQMAIKNRPNPPLK"
     gene            2027..3571
                     /locus_tag="VN968_10800"
     CDS             2027..3571
                     /locus_tag="VN968_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HXL29763.1"
                     /translation="MTTFEWLIALLLGAVLLSALARRIKVPYPTFLAVGGMLLAFVPS
                     GPSWTLEPDLALALFVAPVLLDAAFDTSLRDLRDNWLPVSTLVFIAVGVTTAAVAALA
                     HWLLPDMPWAAAIALGAIVAPPDAAAATAILRQVNLPYRILKILEGESLLNDASALLI
                     YRVAVGAAAVEHFRWSEFAPSIVLALAGSLVAGYLFARTSMLLTRRISDAPSSIIVQF
                     AGTFTVWIVAEHVGLSGILTIVAYAITIARTAPARTPARLRVPSYAVWETAVFVLNVL
                     AFMLIGMQLRPIWTRLDDPVRLNYCLIAAAVLGAVILTRIAWVAIYGTTLRLLIARGF
                     FQPGSSMAVPTVRGGIVVAWCGMRGIVTLAAAFALPESFPYRDLMLLTAFAVVLGSLV
                     IQGLTLRPLISALHLKDENPVASEVARARAVAYRAALEEIDGDPSEEAEILRLEYRAL
                     LLRAEGDPDGGVASGELPADPLRRRAIGAARRSILRQRQSEAIGDDAFHRLEEEFDWA
                     ELSAQG"
     gene            complement(3636..4439)
                     /locus_tag="VN968_10805"
     CDS             complement(3636..4439)
                     /locus_tag="VN968_10805"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXL29764.1"
                     /translation="MQRLKGKTAMVVGAGSIGPGWGNGKATAVTFAREGARVFCVDRN
                     GAAAEETVKIIAAEGGTAAAFTADAANASEVEAMVAACLTAYGRIDVLDNNVGIAEMG
                     NVVDVSEASWDHVFDVNLKSAFLAMKHVIPVMARQGGGSIINISSIASIRHLGISYVT
                     YATTKAAMNQMTRTTAVEYAPKHIRVNAVLPGLMKTPMVEHSAGLAASYAAGDVEAMW
                     RARDAQVPMGHMGDAWDVANAALFLASDESKYVTGIELIVDGGITLKAG"
     gene            4722..5864
                     /locus_tag="VN968_10810"
     CDS             4722..5864
                     /locus_tag="VN968_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HXL29765.1"
                     /translation="MGAAILLCSSGLHLAYGKPFMIVGSDEKVWWDDDGKTVLSAPGK
                     DTVLIVDLANPEDPKIVATLPLKNSIVGPPVNLDIDPTGAVALVADSVDVTKDGDALK
                     QVPDNKIYVIDLKASPPKLAATVTGGKQPSGLSISPSGKMALVANRGDNSISVLSLNG
                     TDVKVTDTLTFPDSVAHVVFTPDGKRALAVRFPAHKVSVLDIAGDKVTYNKLDLPTGQ
                     WPYNVVVTPNGKIALTSDNGGAGSSDGSVDTTSVIDLEANPPRIIDRVVVGDGPEGLA
                     MSPKGDLAVAAILRGSNNKKAFFYQKNGSLSILKIDGKKVTKTQDIEVGGLPEAVLFT
                     PDGKYILAGNYMTQDFSILKVSGTRVTDTGKRFKVPGHPASARMSH"
     gene            6008..7012
                     /locus_tag="VN968_10815"
     CDS             6008..7012
                     /locus_tag="VN968_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL29766.1"
                     /translation="MRSSNFPSRPSRRSVLIGGAAFGALTSMPRRARADQWPSRPVKL
                     VVPFAAGGTTDILARVVAAKASEEFGQQFIVENKAGAGGNIAADAVAKADPDGYTFIV
                     GTPGIHAINQFVFKSMPYDQLKDIAPVIVIARVPNLCSVTNALPVKSVAELIAYAKSK
                     PGELFYGTPGLGSTAHVSCELFKSMTGIEITHVPYKGSAPALTDLIAGRVHLMIDNLP
                     AAQPFAESGSIRPLAISTATRWPLLPDLPTIAEAGVPGYEAASWFTIGAPAKTPDEIV
                     VRLNASVDKFLKSDDGIARLRRLGAEPAGGSPDDMQRYVLAEADKWGKVAKFAGIRPE
                     "
     gene            7015..7278
                     /locus_tag="VN968_10820"
     CDS             7015..7278
                     /locus_tag="VN968_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29767.1"
                     /translation="MGASKIGLARRALAATLLLVLCGSASAQQEDLAIEQQGVARRVL
                     LHRPPEAALLVKLGYHAKMAITPARPSLKRHVEAFRNVGAEAG"
     gene            complement(7225..7704)
                     /locus_tag="VN968_10825"
     CDS             complement(7225..7704)
                     /locus_tag="VN968_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29768.1"
                     /translation="MSHVMKICFGVALLLVTLVPPVQADDFKLSNNQRISCSRGLSAG
                     KLSTATCKSYAYLFNIKTTEYFRCQVSLALTRDNKEVINVQTDGGCTKKPRIFETDSS
                     YSFDATETEPPNTNSFFGPGGYAVWASDNTAQKVRGCIIISSGLGSDISKCLDMTFQ"
     gene            7921..8226
                     /locus_tag="VN968_10830"
     CDS             7921..8226
                     /locus_tag="VN968_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29769.1"
                     /translation="MTRFSTRSFLIAAALTALTTSAFAEAAAPTPWVLTPDMGYGYDK
                     EGKTFSYKMGTNNAKLLLKGAKKVPKGTLFFIGENGQLYMRTGPFLEGDGKFMFGSN"
     gene            complement(8292..9422)
                     /locus_tag="VN968_10835"
     CDS             complement(8292..9422)
                     /locus_tag="VN968_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29770.1"
                     /translation="MDAVVLPSATAGAMAQVVSPQAMAEYRRKLKEYLEARAAFDQEA
                     GAYWSSIADKRRVRNAKRRDRQSIALDDYVLTQPPVYSGPKRPVDPSPSPPEPEEPGR
                     AHKPIPVVADLLKAAAAQFQFTPQRPASEVDFKRAYARVAATAGLTRDQAVRVYSFET
                     GGNGNHDMQSGLSASRPGSRAISTAIGYNQLLTTNTVELLAEQGPEFARALAEKAAQL
                     SGAPRQAMDRKIAVLKRMAAYARSVPDEWAQHEKIGDTPQGWALHAMVLDIDVGPLLQ
                     THKLLTSVVFARAKGFSRPLTAAELEMMNLTGDGTGLDMVTMPQAMREQVPTSNFFQR
                     GGYERNPVAIRNNTVAKLLDVTDRRMDSNSNLPGAKELAAAF"
     gene            complement(9572..10216)
                     /locus_tag="VN968_10840"
     CDS             complement(9572..10216)
                     /locus_tag="VN968_10840"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HXL29771.1"
                     /translation="MTRPFDDTTRRNIAERCAAFARLPASELASQLAPALKRAVVAIA
                     LTEAADAADGTAVLLTLRAAGLRAHRGQWALPGGRCDEGETQVKTALRELHEELGLEL
                     GEENVLGWLDDYPTRSGYLITPVVVWAAAGAAISPNPAEVASVHRIALGDIEQPDAFD
                     FVAIPESSRRVIRFRHAGQFIHAPTAALIYQFREVLAGHDTRVAELEQPVFAWK"
     gene            10306..10731
                     /locus_tag="VN968_10845"
     CDS             10306..10731
                     /locus_tag="VN968_10845"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXL29772.1"
                     /translation="MTDKAAAQLKSSGWTIVDTTGFISLVGPLWQRVVDSEHEYALVA
                     QDKHHNRRGLVQGGLLMTFADRSCGMTARYVSGRPTLATVQLDTHFVDSGKIGDILIS
                     RPRVVRSTRSLIFVTTEVTADKRCIVMASGVFKILKNDT"
     gene            10888..11901
                     /locus_tag="VN968_10850"
     CDS             10888..11901
                     /locus_tag="VN968_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXL29773.1"
                     /translation="MQYRQLGRSGLKISPICLGTMMFGGPTDEATSTRIVAKAREAGV
                     NFIDTADAYSKGNSEQVIGRAISNNRQNWVLATKLANPMGDDPNRGGLSRRWVLQAAD
                     ESLKRLGTDYIDIYYLHKEDHSTPLHETVRAMGELIQQGNVRYFGVSNYRAWRVAEIC
                     NICDAIGIDRPIASQPYYNAMNRMPEVEHFPACGFYGLGLVPYSPLARGVLTGKYRPD
                     APPDKDSRAGRNDTRMMQTEWRPESLQLAQQIRQHAEAKGITAGQFAVSWVLNSSFVS
                     AVIAGPRTEEQWDDYLRALDYRFTAEDEALIDRLVTSGHPSTPGYNDPAYPIEGRRAR
                     TSI"
     gene            12090..12353
                     /locus_tag="VN968_10855"
     CDS             12090..12353
                     /locus_tag="VN968_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29774.1"
                     /translation="MTRSRRETVTFRHPFRIDGIDRRLQAGAYEVVTDEELIEGLSFP
                     CFRLVATMIMVPGAPPHHQSTEMLAMNSVDLADAQRADAVASL"
     gene            12422..12601
                     /locus_tag="VN968_10860"
     CDS             12422..12601
                     /locus_tag="VN968_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29775.1"
                     /translation="MQILSARHVKTEESERLGVVSGWYSTKVSGTFVTGPHTTQEDCL
                     TKIAELNPAPAKKKF"
     gene            12635..12919
                     /locus_tag="VN968_10865"
     CDS             12635..12919
                     /locus_tag="VN968_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 family protein"
                     /protein_id="HXL29776.1"
                     /translation="MALTQGSFQEYDFNRMVVLFTMKNDEANVACAISTDAMDQLEGS
                     SRTPPAQREQQFLRLRDRIEARAARKFSDVELEGNPPGVILRAIDFRAPR"
     gene            complement(13476..14102)
                     /locus_tag="VN968_10870"
     CDS             complement(13476..14102)
                     /locus_tag="VN968_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29777.1"
                     /translation="MDILVILGGLLLALVVAGFSSARSYFEKGRLQGMEEATRELSRG
                     ISSHYELEGQAIPESVAKALKGFNAVAAKRWKKRKGSTDPYHAQLWTVGDAIGEACWM
                     KGHSAGIRRKAPAEGKIRVDLSLTELLQLSWLAHLGFQHMMPNYRGFEIHRFSGEEDA
                     LEGAKAVGKIESVIPAKERPFADLAAQLKNRQKLIFDWWKPMPDRLTA"
     gene            14525..15436
                     /locus_tag="VN968_10875"
     CDS             14525..15436
                     /locus_tag="VN968_10875"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXL29778.1"
                     /translation="MIALRQLNSHRRNSHRRAGGRFAVAVVAALAAAPAAFAADMAAR
                     TPVYAKAPIATDWTGFYLGAGIGARSSQVDGDVSQDSINSFNNFFAGNLCAPGGSSCS
                     GESLNGTAFRISPYLGYNYQFATQWLAGIEGDMGFGSKTTTLANAFYPNTGISSFRGQ
                     DSFAVKTGWDAGLRGRLGFLVTPSFLVYGTAGAAWQHIDATSTCSTDTNSFSFCEPRL
                     FAPSVITDSATKLGWTVGAGIETTLSQNWIVRGEYRYADFGTITHTDTRACGSPPHCL
                     LSSLAITDTLRLRTQTATFGVAYKFGP"
     gene            15452..15898
                     /locus_tag="VN968_10880"
     CDS             15452..15898
                     /locus_tag="VN968_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29779.1"
                     /translation="MDDVSQWFWLVLDQARPNLGRLATWLEAASRAEIEDLAREFRRA
                     KQHVVADYSKGIEVDGVVLSDEATEDFCDWVVARGRDFWQAAVASRADLSGLAREYQR
                     AEEFGKAASRAWNASALGQKYRGSHSPRLLAYAVYDARFGADLDDA"
     gene            16249..17769
                     /locus_tag="VN968_10885"
     CDS             16249..17769
                     /locus_tag="VN968_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL29780.1"
                     /translation="MNRSKQNAAVSRGVAGSDRLWAPRLAQESRLKQLEDSFVRHRVE
                     GEAVEPEIERAVLLGALNRRQDAQAAFIDILRRVPANFSALNEFGALLTDMGAIDAAC
                     RVYAEAIVHHPANPMGHVNLANLLFRASKHAEAREHYETALRIDPNHVQAHQGLGAVL
                     SDLGDRAGARNHFQKGFRGHAISSLPYRGTKPPVALLQLVSSGGGNIPTAAFLDDGTF
                     LTSVIVTDYLDPSIPLPPHQLIFNAIGDADLCEPALEAAAGLIARTSAPVVNDPRAVM
                     KTGRISNARRLGAIAGVVAPRTIAMPRDVLAGPDGASSIVDHRFTFPLLLRSPGYHTG
                     RNFILVASAAELAAAAASLPGNDLLVIEYLDARGKDGNARKYRVMMIAGRIYPLHLAI
                     SRQWKVHYFTSDMADQPDHRLQEAGFLGDMPAALGDKAIAALERIRDALGLDYGGIDF
                     GLGQNGDLLLFEANATMVITPPSPDERWAYRRAAIGKILDAVVAMIMQKSAQAGRA"
     gene            17793..18119
                     /locus_tag="VN968_10890"
     CDS             17793..18119
                     /locus_tag="VN968_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29781.1"
                     /translation="MVAKYSPLTSPKLDRRNQGLEVPERFIEFNLMASMDWEPTWIAR
                     EIALILKSSWSFPGVEHLAESRFRIIRGLGPPCRFICPTRRLGGNGKCAYHDDRSQRG
                     GEMLPR"
     gene            complement(18323..18814)
                     /locus_tag="VN968_10895"
     CDS             complement(18323..18814)
                     /locus_tag="VN968_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29782.1"
                     /translation="MFGQLSRNAKQRLRLFLSECRRRARVLRDRLRQQPDRTNPSSRF
                     VPGFLHSYGAREARGFELLKEWLSPEQLAQYEARSYFEVTGCHSGKRYRISHGNSMNI
                     HELDGAGRPRFGWCFAPKGYLVAGDVMLAQKIALETDERGALAVANKFAVPMGRRNTL
                     FDC"
     gene            complement(18823..19044)
                     /locus_tag="VN968_10900"
     CDS             complement(18823..19044)
                     /locus_tag="VN968_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29783.1"
                     /translation="MATQIVMDRMGDTRHNFDAKNPEALSKAEERFRELTGAGFTAAV
                     RTSSGEPVVQRTFDPNAEETLFFPRLVGG"
     gene            complement(19165..19569)
                     /locus_tag="VN968_10905"
     CDS             complement(19165..19569)
                     /locus_tag="VN968_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29784.1"
                     /translation="MLQGQPVPKSRQVYIRENWLVRHVWLARVAALATTEIRHPQVYV
                     PDPTERLVSKIREFVETHGARLMVGLQRSDDKLVQHLQAERIPFVTFNDAEAYSDRFG
                     AHWTPAGHKLVAERLLRLLSENNVIPTDELLR"
     gene            19982..>20170
                     /locus_tag="VN968_10910"
     CDS             19982..>20170
                     /locus_tag="VN968_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29785.1"
                     /translation="MTRIRGNRTQERVSPLRFIEKEMKGRIVKTGKRIVTAALGGALC
                     LAASMGLSAAASRSPTTMK"
BASE COUNT         3749 a         6373 c         6254 g         3794 t
ORIGIN      
        1 cgatgcacca gccggtttca gtctgccacc agtgatcgat caccggcacc ttcaattgct
       61 tctccgccca gtgcaccgtc ggcggatcgg cgcgctcgcc ggcgagaaac agcgtgcgga
      121 attttgacag atcatactgg cggatgaatt ggccgtcggg gtcttccttg cggatggcgc
      181 ggaacgcggt tggtgctgtg aacagcgcca ccgccttatg ttccgcaatc acccgccaga
      241 acgcgccggc gtccggcgtg ccgatcggct tgccttcata catgatcgag gtcgcgccat
      301 gaatcagcgg gccgtagatg atgtagctgt ggccgaccac ccagccgatg tcggagccgc
      361 accaccagac ctcgccgggg ttgaccccgt agagattgaa catcgaccac ttcaatgcga
      421 cgaggtgccc gccattgtcg cgtaccacgc ctttgggttt gcccgtggtg cccgacgtgt
      481 agaggatgta gagcggatcg gtcgcgagca ccggcacgca ggcggcggat tttttcgccg
      541 acatcgcctt ggttcgcagt tccgaccagt cgtgatcgcg gccgcccacg agttcgcaag
      601 cccactgcgg ccgctgcagg atgatgcagg cctgcggctt gtcgcttgaa agtttgatgg
      661 cttcgtcgag cagcggcttg tactgcacga tgcggccggg ctcgagcccg cagctcgcgg
      721 aaaaaatcag ttttggcttg gcgtcctcga tccgggtggc aagctccttg gcggcaaaac
      781 cgccgaacac cacgctgtgc accgcgccga tccgcgcgca ggccagcatc gcgatcatgg
      841 cttccggcac catcggcata tagaggatga cgcggtcgcc cttggcgacg ccgaaatcac
      901 ccatgatggc agcgagcgtt ttcacctcat gcagcatctg cgcgtacgtc aatctggtga
      961 cgcggcccgc ctgcggcgaa tcgtggatca gcgcgacctg ctcggcgcgg ccattcgcga
     1021 catggcggtc gagcgcgttg taacaggtgt tgaccacggc gcccgcgaac cagcggccat
     1081 agaggccgct cgccggatcg aacacctttt tcgccggctc gatccagtcg atctcgcgcg
     1141 ccgcctcgcc ccaaaagccc tcggggtcga ccagcgagcg gacatgcgct tggtgatacc
     1201 ggctgttgtc ctggatgttc atggcgttgc tcccgcgcga tccgcgccgc cctcgtcatg
     1261 cccggcctgg tgccgggcca tgacgacgcg gggacaatcg caatttattt ggctgccatt
     1321 gtcacgcgaa gcagccttat ttcaagggtg gattaggcct gttcttgatc gccatctgcg
     1381 cgccgatccg ctccagttcc agtgccagcg acctgtaatt ggcgcgcgcc gcctcgtgtt
     1441 gcggattgag tttgatggcg gcgccgaaat cggcgagcgc gcggggccgg tcgccctttt
     1501 tgcgccagag ctcgccgcgg gcgttgaaga tatcggaaag cgccgggtcg agccgcagcg
     1561 cggtgtcgta gtcgccgatg gcgcggtcga tcatgtcctt gcggccgtag gcgccggcgc
     1621 gcgcgatcag cgccttgatg cggtcggccc gcagcgtctt gtcgttgtcg accagcgcgc
     1681 cgcagacggc aatgatcttg tcggcgtcat cggcggccgc agcggcgacg cacggcgcgg
     1741 gatcgatctg cggatcggtg acgggctcat tgccggttgc gcaggcgtgg tacgccggtg
     1801 ttgcccacag gaccgacgcg agcgccacgc tccggatcag gcctgacaaa ccatctcgca
     1861 tcaatctgtc ccgcgtatcg gtgttcgata tttcgctatt gcaacgagcg tagcaagcgg
     1921 caggcggttc ggacaatgat ggatttcggg cgattagacc gggcgcgttt cgatgctaga
     1981 ttgccggcga ctgaccgaca agacccggcg acaaggaccc ccaggtttga cgacattcga
     2041 atggctgatt gccttgctgc ttggcgcggt gctgctgtcg gcgctggccc ggcggatcaa
     2101 ggtgccctac ccgacctttc tcgccgtcgg cggcatgctg ctggcctttg tgccatccgg
     2161 cccgtcctgg acgctggagc ccgatctggc gctggcgctg ttcgtggcgc cggtcttgct
     2221 ggatgccgcc ttcgacacct cgctgcgcga cctccgggac aactggctgc cggtctcgac
     2281 gctggtattc atcgcggtcg gcgttaccac cgccgcggtc gcggcgctcg cccattggtt
     2341 gctgccggac atgccgtggg cggccgcgat cgcactcggc gccatcgtgg cgccgccgga
     2401 tgctgccgcg gcgaccgcga tcctgcgcca ggtcaacctg ccctatcgga tcctaaaaat
     2461 cctcgaaggc gaaagcctgc tcaacgacgc cagcgcgctg ctgatctatc gcgtcgccgt
     2521 cggcgccgcc gcggtggagc atttcaggtg gagcgagttt gcgccgtcga tcgtcctggc
     2581 gctggcgggg agcctggtgg ccggctacct gttcgccaga acttcgatgc tgctgacccg
     2641 gcggatcagc gacgcgccaa gctccatcat cgtgcagttc gccggcacct tcacggtgtg
     2701 gattgtcgcc gagcatgtcg gcctctccgg cattctgacc atcgtcgcct atgcgatcac
     2761 cattgcgcgc accgctccgg cgcgcacgcc ggcgcggctg cgcgtgccat cctatgcggt
     2821 gtgggaaacc gcggtgtttg tcctgaacgt gctggcgttc atgctgatcg gcatgcagct
     2881 ccgcccgatc tggacccggc tcgacgatcc cgtgcgcttg aactattgcc tgatcgccgc
     2941 cgccgtgctc ggcgccgtga tcctgacgcg catcgcctgg gtggcgatct acggcaccac
     3001 gctgcggttg ctgatcgcgc gcggattctt tcagccgggc agttcgatgg ccgtgccaac
     3061 cgtgcgaggc ggcatcgtgg tggcctggtg cggcatgcgc ggcatcgtga cgctggcggc
     3121 ggcgttcgcg ctgccggaaa gttttccgta tcgcgatctg atgttgttga cggcgtttgc
     3181 cgtggtgctg ggctcgctgg tgatccaggg cctgaccctg cggccgctga tctcggcgct
     3241 gcaccttaag gacgagaacc cggtggcatc ggaagtggcg cgcgcccgcg ccgtcgccta
     3301 tcgcgcggcg ctggaggaaa tcgacggcga cccgtccgaa gaagccgaaa tcctcagact
     3361 tgaatatcgc gcgctgctgc tgcgcgccga gggcgacccc gatggcggcg ttgccagcgg
     3421 cgaactcccg gccgatccgc tgcggcgtcg cgccatcggc gcggcgcggc gctcgatcct
     3481 cagacagcgc cagtccgaag cgatcggcga cgacgccttt catcggctcg aggaagaatt
     3541 cgactgggcc gaattgagcg cgcagggcta gctacaaatt ctgcgtcgtc ccggcgaacg
     3601 ccgggacgac gagaggggtg tttcacttga cgttactaac ccgccttcag cgtgatcccg
     3661 ccatcgacga tcaactcgat ccccgtgacg tatttggact cgtcggaagc cagaaacagc
     3721 gccgcgttgg cgacatccca ggcgtcgccc atgtggccca tcggcacctg cgcgtcgcgg
     3781 gcgcgccaca tcgcctcgac atcgcccgcg gcatagctcg cggccaatcc ggcggaatgc
     3841 tcgaccatcg gcgtcttcat caggcccggc aagacagcat tgacccggat atgtttcggc
     3901 gcgtattcga ccgccgtcgt gcgcgtcatc tggttcatcg cggccttggt ggtggcatag
     3961 gtgacgtagg aaataccgag atggcggatc gaggcgatcg acgagatatt gatgatcgag
     4021 ccgccgccct gccgcgccat cacggggatg acgtgcttca tggcaaggaa ggcgcttttc
     4081 agattgacgt cgaagacgtg atcccagctc gcttccgaga cgtcgaccac gttgcccatc
     4141 tcggcgatgc cgacattgtt gtcgagcacg tcgatgcggc cataggccgt gaggcatgcc
     4201 gccaccatcg cctcgacctc gctggcgttg gcggcatcgg cggtgaaagc cgccgccgtg
     4261 ccgccttcgg ccgcgatgat tttgacggtc tcttccgcgg cggcgccgtt gcgatcgacg
     4321 cagaacaccc gcgcgccctc gcgcgcaaaa gtcaccgcgg tcgccttgcc attgccccag
     4381 cccggcccga tcgaaccggc gccgaccacc atcgctgtct tgcccttgag ccgttgcatc
     4441 gacttctccc tctgagataa ctgtagcccg gattacacac gcgcatcgtc ataacattga
     4501 cgccgaccgg atgcccagat cggccggccg aaagcccggg tcaacaatgt acgggacggc
     4561 gccctatctc gcggtcgacg gttactgcgc ttcgcactgg caacaggaca tttccccgcg
     4621 tactctaggg ctgcaaaacc ggcccatcag aggccggttc tatccgggag aacgcttcaa
     4681 tgcctgttac tcgtaccgtc ggactatgct tgggagcatc cttgggagcg gcgatcctgc
     4741 tgtgcagctc cggccttcat ctcgcttacg gaaaaccgtt catgatcgtc ggcagcgacg
     4801 agaaagtctg gtgggacgat gacggaaaga cggtcctgtc ggcgccgggc aaggacacgg
     4861 tcctgatcgt cgatctcgcc aatcccgagg atccgaagat cgtcgccacc ctgccgctga
     4921 agaattcgat cgtcggaccg ccggtcaatc tcgacatcga tccgaccggc gcggtcgcgc
     4981 tggtggcgga ttcggtcgat gtcaccaagg atggcgacgc gctgaagcag gtgccggaca
     5041 acaagatcta cgtcatcgac ctcaaggcga gcccgcccaa actggcggcg acggtcaccg
     5101 gcggcaagca gccgtcaggc ctgagcatca gtccgtccgg caagatggcc ctggtcgcca
     5161 accgcggtga caattcgatc agcgtgctct cgctcaacgg caccgacgtc aaggtcaccg
     5221 acaccctcac cttccccgac agcgtcgcgc atgtggtgtt cacgccggac ggcaagcggg
     5281 cgctcgccgt gaggtttccc gcacacaagg tctcggtgct cgatatcgcc ggcgataagg
     5341 tgacctacaa caagctcgat ttgccgaccg gccagtggcc ctacaatgtc gtggtcacgc
     5401 ccaacggcaa gatcgcgctg acgtccgaca atggcggcgc cggctcgtcc gacggcagcg
     5461 tcgacaccac cagcgtgatc gatctcgagg ccaacccgcc gcgcatcatc gatcgggtgg
     5521 tggtcggcga cggcccggaa gggctggcga tgtcaccgaa gggcgatctc gcggtcgccg
     5581 ccatcctgcg cggctccaac aacaagaagg cgtttttcta ccagaagaac ggcagcctct
     5641 cgatcctgaa gatcgacggc aagaaggtga ccaagaccca ggacatcgag gtcggcggat
     5701 tgcctgaggc ggtcctgttc acccctgatg gcaagtacat tctggccgga aactacatga
     5761 cccaggattt ctcgatcctg aaagtcagcg gcaccagggt caccgacacc ggcaagcgtt
     5821 tcaaagtgcc ggggcatccg gcttcggcgc gcatgagcca ctaggatcgc gcgcaccggg
     5881 atcgcaagga gcgggtgacc gatttagagg caagccaggc ctcgcgcgag acgctgaccg
     5941 acgctataga agcgtccggt cggcgttttg ccgtgaccgg agcacttcaa caaggacacc
     6001 attgcgcatg cgatcatcga atttcccgtc gcgtcccagc cgccgctccg tgctgatcgg
     6061 cggcgcagca ttcggcgcgc tgacctcgat gccgcgcagg gcacgcgccg atcagtggcc
     6121 gtcacggccg gtcaagctgg tggttccgtt cgccgccggc ggcaccaccg atattttggc
     6181 gcgggtcgtc gccgccaagg cctcggagga attcggccag cagttcattg tcgagaacaa
     6241 ggccggcgcg ggcggcaaca tcgcggccga tgccgtcgcg aaggccgatc ccgacggcta
     6301 caccttcatc gtgggcacgc cgggcattca cgccatcaac cagttcgtgt tcaagagcat
     6361 gccgtacgac cagctcaagg atatcgcgcc ggtgatcgtc atcgcccggg tgcctaacct
     6421 ctgctcggtc accaatgcgc tgccggtgaa atcggttgcc gaactgatcg cctatgccaa
     6481 gtcgaagccg ggcgaattgt tctacggcac gccggggctc ggcagcaccg cgcatgtctc
     6541 atgcgaattg ttcaaatcga tgacgggtat cgagatcacc cacgtgccct ataaaggcag
     6601 cgcgccggcg ctgaccgacc tgatcgccgg ccgcgtccat ctgatgatcg acaatcttcc
     6661 ggcagcgcag ccgttcgccg aatccggctc gatccgtccg cttgcgattt cgaccgcgac
     6721 gcggtggccg ctattgccgg accttccgac catcgccgaa gccggcgtgc ccggctacga
     6781 ggcggcgtcg tggttcacca tcggcgcgcc cgcgaaaacg cccgatgaga ttgtcgtccg
     6841 gctcaacgcc agcgtcgaca aatttctgaa atccgacgac ggcatcgcgc gcctgcgcag
     6901 gctcggcgcc gagccggccg gcggatcgcc cgacgacatg cagcgttacg tgctcgccga
     6961 agccgacaag tggggcaagg tcgccaaatt cgccggcatc aggccggagt gagtttgggg
     7021 gcgagcaaga tcggactggc acgccgtgcg ctggccgcaa cgttgctgct tgttctgtgc
     7081 ggatcggcat cggcgcagca ggaagatctc gccattgaac agcagggtgt cgcccggcgc
     7141 gtgctgctgc atcggccgcc cgaggcggcc ttactcgtga agctcggcta tcacgcgaag
     7201 atggctatca cgcctgcccg accatcattg aaacgtcatg tcgaggcatt tcgaaatgtc
     7261 ggagccgagg ccggatgaaa tgatgatgca gccgcggact ttttgcgcgg tgttgtcgct
     7321 ggcccacacc gcgtagccgc cggggccgaa aaacgaattg gtgtttggcg gctcggtctc
     7381 ggtagcgtcg aacgagtagc tggaatcggt ctcgaagatg cgaggcttct tggtgcagcc
     7441 gccgtcggtc tggacgttga tgacttcctt gttgtcccgc gtcagcgcca ggctgacctg
     7501 gcaccggaaa tattccgttg tcttgatgtt gaagaggtag gcgtaggatt tgcaggtcgc
     7561 ggtgctgagc ttgccggcgc tcaggccgcg gctgcaggaa attcgctggt tgttgctgag
     7621 cttgaagtca tcggcttgaa cagggggaac cagcgtcacc agcaacagcg cgacaccaaa
     7681 acaaatcttc atgacgtggc tcatccgaat catcccccgt gacgtttttt caaaaacggt
     7741 tctagactga aaccgaaaga tagtcgcgaa gtcggatgcg ggaaatcaca aagtctaaag
     7801 tctaatgtag cgcgccgcca tgaatggacc ggtcatattg acgggtgaat ggcgttcgtg
     7861 gtttgttcaa gcatgggcaa accgaccagc tttgaagacc ggccgccggg gagaacgatg
     7921 atgacgagat tttcgaccag gagctttttg atcgcagccg cgctgaccgc gctgacgaca
     7981 tccgcatttg cagaagccgc cgcgccgacg ccctgggtgc tgacgcccga catgggctat
     8041 ggctacgaca aggaaggcaa gacgttttcc tacaagatgg gcaccaacaa tgccaagctg
     8101 ctgctgaagg gcgcgaaaaa ggtgccgaag ggcacgttgt ttttcatcgg cgagaacgga
     8161 cagctctata tgcggaccgg accgttcctt gaaggcgacg gcaagttcat gttcgggagt
     8221 aattgagcgg attctctctt cccttctccc cttgtgggag aaggtggcac cgcccattaa
     8281 aacacagcct actaaaacgc cgccgcgagt tccttcgcgc ccggcagatt gctgttgctg
     8341 tccatccggc ggtcggtgac atcaagcagt ttcgccacgg tgttgttgcg gatagcgacc
     8401 ggattgcgct cgtaaccgcc gcgctggaaa aaattcgagg tcgggacctg ctcgcgcatc
     8461 gcctgcggca tcgtcaccat gtcgagcccg gtgccgtcgc cggtcagatt catcatctcc
     8521 agttcggcgg cggtcagcgg gcggctaaaa cccttggcgc gcgcgaagac gacggaagtg
     8581 agcagcttgt gggtctgcag cagcgggccg acatcgatgt cgagcaccat cgcatgaagc
     8641 gcccagccct gcggcgtatc gccgattttc tcgtgctgcg cccattcgtc cggcaccgaa
     8701 cgcgcatagg ccgccatccg cttcaacacc gcgatcttgc gatccattgc ctgacgcggc
     8761 gcgcctgaca attgcgcggc cttttccgca agcgcgcggg cgaattcggg gccctgctcg
     8821 gcgagcagtt cgacggtgtt ggtggtgagc agctgattgt agccgatcgc ggtcgagatc
     8881 gcacgcgagc ccggccgcga ggcgctcaat cccgattgca tgtcgtgatt gccgttgccg
     8941 ccggtctcga acgaatagac ccgcaccgcc tgatcgcgcg tgagccccgc cgtggcggcc
     9001 acgcgggcat aggcccgctt gaaatcgact tcgctggcgg gccgctgcgg ggtaaactgg
     9061 aactgcgccg cagccgcctt caacagatcg gctaccaccg gaatgggttt gtgcgcgcgg
     9121 cccggttcct cgggctcggg cggcgatggc gatgggtcca ccggccgctt cggcccggaa
     9181 tacaccggcg gttgcgtcag cacataatca tcgagcgcga tcgactggcg atcgcgccgc
     9241 ttggcgttgc ggacccgccg cttgtcggca atcgaactcc agtaagcgcc ggcttcctgg
     9301 tcgaacgccg cgcgcgcttc gagatactcc ttcagcttgc ggcgatattc cgccatcgcc
     9361 tggggcgaca ccacctgcgc catcgcgccc gccgtcgccg acggcaatac aacggcatcc
     9421 atcgcgaccg cctgctgagc gatcagcagc agcgcgagtg caaagaacct gcggtgagaa
     9481 cgaatcgcat cagggcgcat cggggtgttg tagagcaact gtggcgcgca agtcagcccc
     9541 cgaaaccgtg ctattgcggc gtgggagacg tctacttcca ggcaaatacc ggctgttcca
     9601 gctcggcgac gcgggtatcg tggccggcca gcacttcgcg gaactgatag atcagggcag
     9661 cggtcggcgc gtggatgaac tggccggcgt gacggaaccg gatcacccgc cgcgagcttt
     9721 cgggaatggc gacgaagtcg aacgcgtccg gctgctcgat atcgcccagc gcgatgcgat
     9781 gcaccgaggc gacctctgcc ggattgggcg aaatggcggc gccagcggcc gcccacacca
     9841 cgaccggcgt gatgagatag ccggagcgcg tgggatagtc gtcgagccaa cccagcacat
     9901 tctcttcgcc gagctcaagg ccgagttcct cgtgaagttc gcgcagcgcc gtcttgacct
     9961 gcgtctcgcc ctcgtcgcag cgtccgcctg gcagcgccca ttgaccgcga tgggcgcgca
    10021 agcccgccgc gcgcagggtc aacagcacag ccgttccgtc ggccgcatcg gcagcttcgg
    10081 tgagcgcgat ggcgacgacg gcccgcttca acgccggcgc tagttggctt gccagttcac
    10141 ttgccggcag ccgtgcaaac gccgcgcaac gctctgcgat attccgccgg gtggtatcat
    10201 caaatggccg ggtcatcccg cttgactaca acacgccggc cattgatgaa atgaccacaa
    10261 gacctgcaag acccgaattc gccgccgaga ccggaaagac ccgacatgac cgacaaggcc
    10321 gcggcgcagc tcaaatcctc cggctggacc atcgttgaca ccaccgggtt tatcagtctg
    10381 gtcgggcctt tatggcagcg ggttgtcgat agcgagcacg aatatgcgct cgtcgcgcag
    10441 gacaagcacc acaaccgccg cggcctggtc cagggcgggc tgttgatgac attcgcggac
    10501 cgcagctgcg gcatgacggc gcgatacgta tcgggccggc cgacactggc gacggtccag
    10561 ctcgacaccc attttgtcga cagcggcaag atcggcgata tcctgatctc caggccgcgc
    10621 gtggtccgct cgacccgcag cctgattttc gtgaccaccg aggtcaccgc cgacaagcgt
    10681 tgtatcgtga tggccagcgg cgtgtttaag atattgaaga atgatacgta gcgcttgcgg
    10741 ccatcgcgag gggccacgaa acacactccg tcattccggg atggtccgaa ggaccagacc
    10801 tcagatgtgc aattgcacat cggggaatct cgagattccg ggttcgcgct gcgcgcgccc
    10861 cggaatgaca ctgaaaggaa tattccgatg caatatcgtc aactcggccg cagcggcctg
    10921 aaaatctcgc cgatctgtct cggcaccatg atgttcggcg ggccgaccga tgaggcgaca
    10981 tcgacgcgga tcgttgcgaa ggcgcgggag gccggcgtca atttcatcga taccgcggac
    11041 gcctattcaa aaggcaattc cgaacaggtg atcggccgcg ccatttccaa caaccgccag
    11101 aactgggtgc tcgcgaccaa actcgccaac cccatgggcg acgatcccaa tcgcggcggg
    11161 ctgtcgcggc gctgggtatt gcaggccgcc gacgaaagcc tgaagcggct cggcaccgac
    11221 tacatcgaca tctattattt gcacaaggag gatcattcga cgccgctgca cgagaccgtg
    11281 cgcgcgatgg gcgaactgat ccagcaaggc aacgtgcgtt acttcggcgt ctcgaactat
    11341 cgggcctggc gggtcgccga aatctgcaac atctgcgatg ccatcggcat tgaccgcccg
    11401 atcgccagcc agccctatta caacgcgatg aaccgcatgc ctgaagtgga gcacttcccg
    11461 gcctgcggat tctatggcct cggcctcgtg ccctacagcc ccttggcgcg cggcgtgctg
    11521 accggaaaat atcgccccga tgcgccgccc gacaaggaca gccgcgccgg ccgcaacgac
    11581 acccgcatga tgcagaccga gtggcggccg gaatcgctgc aactggcgca gcaaatccgg
    11641 cagcatgccg aggcgaaggg gatcaccgcc gggcagttcg cggtgtcgtg ggtgctgaac
    11701 tcttcgttcg tctcggccgt gatcgcaggc ccgcgcaccg aagagcaatg ggacgattat
    11761 ttgcgcgcgc tcgactatcg ttttacggcc gaggacgaag ccctgatcga tcgcctcgtc
    11821 acatcgggcc atccttccac gccgggctac aacgatccgg cgtatccgat cgagggacga
    11881 agggcacgga cctcgatttg aaaaagaaag tcgtggctat ttggggctaa aaggcgcaca
    11941 cgtccgctgt tcgcctcacg cgccgggtgc gcttccgtct ccgcggggtt gcgaacagac
    12001 gatttgcaac aatagctggc gaaacggcgc gaaagcggtt caattttcat tgaatcggct
    12061 gagcctgcgc caatttcgag gtggtgagct tgacgaggtc gcggcgcgaa acggtaactt
    12121 tcaggcatcc gttccggatc gacggcatcg atcgccggct gcaggcggga gcctatgaag
    12181 tcgtcaccga cgaagagctg atcgaagggc tgtcgtttcc ctgcttccgc ctcgtcgcca
    12241 ccatgatcat ggttcccggc gcgccgccgc accatcagtc gaccgagatg cttgccatga
    12301 actcggtcga tctcgccgat gcgcagcggg ccgacgcggt ggcatcgcta tgaacgcgca
    12361 tcccggatca atcgaaacca ctcgccgcct ccggaataac atcgcacctc aaggaatccc
    12421 catgcaaatt ctctccgcgc gtcacgtcaa aaccgaagag tccgagcgtc tcggcgtcgt
    12481 ctccggctgg tacagcacca aggtgagcgg caccttcgtc accggccccc ataccacgca
    12541 ggaagattgc ctgacgaaaa tcgccgagct caatccggca ccggccaaga agaaattctg
    12601 aactgacagt ctgttcaatc gaggagactt gaagatggcg ctgacccaag gcagctttca
    12661 ggaatacgac ttcaaccgga tggtggtgct gtttacgatg aagaatgacg aggccaatgt
    12721 cgcctgcgcc atttccaccg acgcgatgga tcagctcgag ggctcgtcgc gaacaccgcc
    12781 ggcccagcgc gagcagcaat tcctgcggct gcgcgaccgg atcgaggcgc gcgcggcgcg
    12841 gaaattcagc gatgtcgaac tggaaggcaa ccctccgggc gtgatcctgc gcgcgatcga
    12901 tttccgcgcg ccgcgctgaa tgatcgggcg acagtgagcg gggcaagatc ccaccatccg
    12961 gacttgcgga ggatgccgcc cgacatccga aaactagtta gcgcttcctc tcgggaatcc
    13021 cgatgcaggt ggtcgtgccg tcggttcccc tggcggccgt gctgcagggc acggccctga
    13081 cggtctcggt ggagatcccg tcactggcaa cgatgtcccg gccggtttgg ctattcggaa
    13141 gcagcgggcc gcctggctgc ggaaccaagt tggggttccc ttgtgtcccc gttgcggacg
    13201 gcgccaattc gagggtcgag ggcgggggac tgcctgcagt ccccgttgtc tgttgggcaa
    13261 atcccgctaa cggtagcatg gtagccgcga cggcagcgat aagggttact ctgcgcattg
    13321 aagcctccct acggatgcat caatagaatg gaatgttccc gcaaaagttc aacctcggcc
    13381 aaggattttg aaggatcttg aaggattttg ccggtcacac ggcgatgaga cccgtgagcg
    13441 gctttttccg cgatgccgcg aatgctccgc tgctatcaag ccgtcagccg atccggcatt
    13501 ggcttccacc aatcaaagat cagcttctgc cggttcttga gctgagccgc gagatcggcg
    13561 aacggccgct ccttcgcagg gataacgctc tcgatctttc cgacggcctt ggcgccttcc
    13621 agcgcatctt cctcgccgct gaagcggtga atttcgaagc cacgatagtt cggcatcatg
    13681 tgttgaaagc cgagatgcgc caaccagctg agctgcaata gttcggtgag cgaaagatca
    13741 acgcggatct ttccctccgc gggcgccttt ctccggatgc cggcggaatg ccccttcatc
    13801 cagcaggcct cgccaatcgc gtcgccaacc gtccaaagtt gcgcgtgata cggatcggtc
    13861 gagcccttgc gcttcttcca gcgtttcgca gcgacggcgt tgaaaccctt gagggctttt
    13921 gccacgctct cgggaatggc ctgaccttcg agttcgtagt gcgaactgat cccccgcgag
    13981 agttcgcggg tggcttcctc catgccctgg agccggccct tttcgaaata cgatcttgcg
    14041 ctggagaagc cggcgacgac gagcgccaac agcaacccac ccagtatgac cagaatatcc
    14101 accgccccaa atcccacttt ggcccgaagc acattcgagc cgatcgcccc atgtcaaatt
    14161 taaaccaact tcgttaatgc aaccgtaagg ggcggtggcg cagcagggcg cgtgttcaag
    14221 cctggcaaag ctcatcggct gagagatttc ggctgcgtcc aaatttagtt cggcgagcga
    14281 caagaatcac ggtggcacgg cggtcatgcc ttgcgagtcg tcaccgtgat ccgcgccaaa
    14341 aacctgttgc gcaaaaacaa tgatcggcga aaaggcgcat cgatttcggt cccgtccaat
    14401 gcggtggagg ggtgagttcg gcccctgtta aagacagcaa acggaggctt gcgtagcgtc
    14461 ccccttgaag cgcggtccga acaagacgga ttcgcgcgct gtttcagacc agcgggagct
    14521 gcacgtgatt gcactgcgcc aattgaattc ccatcgtcgc aattcccatc gtcgcgctgg
    14581 cggtcgattt gccgtcgccg tcgttgccgc actggccgcg gcgccggcgg cgttcgccgc
    14641 cgacatggcc gcgcgcacac cagtgtatgc caaggcgccg atcgccactg actggaccgg
    14701 gttctatctc ggcgccggca tcggcgcccg ctcgtcacaa gtggacggcg atgtctctca
    14761 agatagtatt aatagcttca acaatttctt tgccggcaac ctctgcgcgc ccggtggttc
    14821 ttcgtgcagc ggggagtctc tgaacggcac cgcattcagg atcagtccct atctcgggta
    14881 taattatcag tttgcgaccc agtggctcgc cggcattgaa ggcgacatgg gtttcggcag
    14941 caagacgaca acccttgcca acgcgtttta tcccaacacc ggcataagta gctttcgcgg
    15001 ccaggactca ttcgcggtca agaccggctg ggacgcgggc ctgcgggggc gtcttggctt
    15061 cctggtcacg ccgtcctttc ttgtttacgg cacggcgggt gccgcctggc agcacattga
    15121 cgcgacatcg acatgcagca cggacaccaa tagcttttct ttctgtgagc ccaggctttt
    15181 tgcaccctca gtcatcaccg actccgcaac caagcttggt tggacggtcg gcgccggcat
    15241 cgagaccacg ctgtcgcaaa actggatcgt gcgcggcgaa tatcgctatg ccgatttcgg
    15301 gaccatcacc cacaccgata cgcgcgcctg tgggtccccc ccgcactgcc tgctatcatc
    15361 tcttgcaatc accgacaccc tgcgcctgag gacccagacg gcgaccttcg gcgtcgccta
    15421 taaattcggc ccatagggcg gagcggggcg cgtggacgac gtttcgcaat ggttttggct
    15481 cgttctggac caggcgagac cgaatcttgg ccgtcttgcg acctggcttg aggccgcctc
    15541 gcgcgcggag atcgaggatc ttgccagaga attccggcgc gcgaaacagc acgtggttgc
    15601 ggactattcg aaagggattg aggtcgacgg cgtcgtcttg tcggacgagg ccaccgagga
    15661 tttctgcgat tgggtcgtgg cgcggggccg ggatttctgg caggccgcgg tcgcgtcccg
    15721 cgccgatctc tccggattgg cccgtgaata tcaacgcgcg gaagaattcg gcaaggcggc
    15781 cagccgggcc tggaacgcct ctgctctcgg acaaaagtac cgaggctccc actcgccgcg
    15841 cctgctcgcc tatgccgtct atgatgcgcg atttggcgcg gatcttgatg acgcctagat
    15901 tgagctgcaa cagcgccagc aagatttctg ccgcgcttcg agcgccgcct gccacgctcg
    15961 tttcaagctg agacgagatc ggcgaatgcc cgggccacga ggacactgat tgcctgcgaa
    16021 gggctttcat cgctcaacag agtcatcaaa cctgtcccgg ccgccttgcc acggccgctc
    16081 cagtttagct ccggagcggc ggtttacgac agccatattc atggggggcg tggtgggagc
    16141 gggcccggct ggtcccgagg accgacgagc caggtgtcgc atgcgcggtg cgaatcagcg
    16201 gacaagtggt atgagtgcaa attctcgaca tcgagccttg cacgcaccgt gaacagatcg
    16261 aagcagaacg cggcagtctc gcgcggcgtt gccggcagcg accgcctgtg ggcgccccga
    16321 ttggcgcagg aatcgagact gaaacagctt gaggattcct tcgtgcgtca ccgcgtcgag
    16381 ggcgaggccg ttgagcccga gatcgaacgc gcggttctcc ttggcgccct caatcggcgt
    16441 caggatgccc aggccgcttt catcgatatc ctgcgaagag tgcccgcgaa tttcagcgcc
    16501 ctcaacgaat tcggcgccct gctgacggat atgggcgcga tcgacgcggc ctgccgggtc
    16561 tatgccgagg cgatcgtcca ccatcccgcc aatccgatgg gacatgtgaa cctggcaaac
    16621 ctgctgtttc gcgccagcaa gcacgccgaa gcacgcgagc attacgagac ggctttgcgg
    16681 atcgatccga atcacgtgca ggcgcatcag ggactgggcg ccgtgctctc cgacctcggc
    16741 gaccgcgccg gcgcccgaaa tcattttcag aaaggttttc gcggccatgc catttcaagc
    16801 ttgccgtatc gcgggaccaa gccgccggtg gcgttgctgc aactggtctc atccggcggc
    16861 ggcaatatcc cgaccgctgc gttcctggac gacggcactt ttctgacctc ggtgattgtc
    16921 acagactatc tcgatccgtc gatcccgctg ccgccgcatc aactgatctt caacgcgatc
    16981 ggggatgcgg acctctgtga acccgcgctg gaggcggccg ccggcctgat cgcgcggacc
    17041 agcgccccgg ttgtcaacga tccccgcgcg gtgatgaaaa ccggccgtat cagcaacgcc
    17101 cggcggctcg gcgccatagc gggagtggtg gcgccgcgga ccatcgcgat gccgcgcgac
    17161 gtcctcgcgg gccccgatgg cgccagttcg atcgtggacc accggtttac gtttccgctg
    17221 ctgttgcggt ctcccggcta tcataccggg cgcaacttca tcctcgttgc ctcggcagcc
    17281 gaactggccg cggcggcggc gagcctgccg ggaaacgatc tcttggtgat cgaatatctc
    17341 gacgcccgcg gcaaggatgg caacgcgcga aaataccgcg tgatgatgat cgcaggtcgg
    17401 atctatcccc tgcatcttgc gatatcccgg caatggaagg tgcattattt cacgtccgac
    17461 atggccgacc agccggacca tcggctgcag gaagcgggct ttctcggcga tatgccggca
    17521 gccctcggag acaaggcgat cgcggcgctt gaacggatcc gggacgccct cggcctcgac
    17581 tatggtggaa tcgatttcgg gctcggtcag aacggcgatc tgttgctgtt cgaggccaac
    17641 gcaaccatgg tgatcacccc gcccagcccc gacgagcgct gggcttatcg gcgcgccgcc
    17701 atcggcaaga tactcgacgc cgtcgtcgcc atgatcatgc aaaaatcggc gcaggcgggt
    17761 cgagcgtagg gtcatgatca acggcatttg ccgtggttgc caaatattcg cctctcacct
    17821 ctccaaaact cgatcgtcgc aatcaaggct tggaagttcc cgaacgcttt atagaattca
    17881 atttaatggc gtcgatggat tgggagccta catggattgc acgcgaaatc gcccttatac
    17941 tgaaatcatc atggagtttt cccggtgtcg aacacctcgc tgaaagccgc tttcgcatta
    18001 ttcgtggtct tggccctccc tgccgcttta tttgcccaac acgcaggctc ggcggcaacg
    18061 ggaaatgcgc ctatcacgac gatcgatccc agcggggtgg cgaaatgctt ccaagatagc
    18121 tccgctaccg ccgccacgga tagccgtcag cgtcccgaaa ttgttcattc caacggcgtt
    18181 tgctgggccc cgcagaatac cgctgacggt ccagaagttt gagtgccgtg aagacgagca
    18241 aacctgccgc gcgaatgggc gcgaaccggt cggccccccg gatcaagtcc gagggcaggc
    18301 tttcgcggga aaacgctctc gttcagcaat cgaaaagcgt gtttcgtcga cccattggca
    18361 ccgcaaattt gttcgccacg gccagcgcgc cacgctcatc ggtttccagc gcaattttct
    18421 gtgccagcat gacatctccg gcgacaaggt aacctttggg cgcaaagcac cagccaaaac
    18481 gcggacggcc ggcgccgtcg agctcgtgga tgttcatcga attgccgtgg ctaatccggt
    18541 atctttttcc gctatggcag ccggtgacct caaaatagct tctcgcttca tattgcgcga
    18601 gctgctcagg agatagccat tccttcaaca gctcaaagcc gcgcgcttcc cttgccccgt
    18661 agctgtggag aaagcccggg acaaatcgcg aagatgggtt agtgcgatca ggctgctgcc
    18721 gcaatctgtc gcgcagaact cgcgcccggc gccggcattc cgacagaaat agtcggagac
    18781 gctgcttcgc gttgcgcgac agttgcccaa acatggccga agttagccac ccacgagccg
    18841 cggaaaaaac agcgtctctt cggcattggg atcaaacgtg cgctgcacga ccggctctcc
    18901 ggacgaggtg cgcaccgccg cggtaaagcc tgcacctgtc aactccctga agcgctcctc
    18961 ggccttcgag agcgcttcag ggttctttgc atcgaaattg tgccgggtat cgcccatgcg
    19021 atccatcacg atttgtgtgg ccatcgaaat cctccgttgc aaggtaccgg aattctacgc
    19081 gcggtttcga ctggcgcgca aacttaacaa cgcgcttgcg gtaaactgac cgtgagccga
    19141 ggctcatcga tcatcgtgcg gatgctaacg gagcagctca tcggtcggga tgacgttgtt
    19201 ttccgatagc agccgcagca ggcgttcagc gaccagcttg tggccggccg gcgtccaatg
    19261 ggcgccgaag cgatcggaat aggcttcggc atcgttgaag gtgacgaatg gaattcgctc
    19321 ggcctgcaga tgttggacca gtttgtcgtc gctccgttgc aggccgacca tcagcctggc
    19381 gccatgggtt tcgacgaatt cgcggatctt gctgaccagt cgttcggtcg ggtcgggcac
    19441 atagacttgc ggatgccgga tttccgtggt ggcgagtgcg gccacgcggg cgagccacac
    19501 atgacgcacc agccaattct ccctgatgta gacttggcgc gatttgggaa ctggctgccc
    19561 ctgcaacacg agcgtgccat ccggcgcggt cgcaaagtaa gctgatagcc atcgtagcga
    19621 atgttggtag cgttatccaa ccggtcgttt gagccaggca ggtcgaccgc agcgcttggt
    19681 ggacccgttc acgcgccaat gggatgcctc atccgacacc tgctccgcaa acctcaggcg
    19741 cgtaccaagt cctgttgcct gatccggtta ttaagagtgg ccttgcggcg aagatatgtt
    19801 gcccaggggg caggttgtcg caaagccaaa cagccaaagc aaaacaatat cttaacacta
    19861 cccagatcga cgtgggcgcc gatttgttgt gcgtcgcaac attgtgtgtt gcaatgcaat
    19921 ctcgggaaat ctatgttgca ttgcagcacg gcagcgggac gcgccgagga cggaaattgc
    19981 gatgacgcgg attcgtggca atcgaactca agaacgcgtt tccccgctta ggttcatcga
    20041 aaaggaaatg aagggtagaa tcgtgaaaac aggaaagcgt attgttaccg cggcgctcgg
    20101 gggagcgctg tgtctcgcag caagcatggg cctatccgcg gccgcgtccc ggtcgccgac
    20161 gaccatgaag
//
LOCUS       DATNOI010000280         2065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_189790,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000280 DATNOI010000000
VERSION     DATNOI010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189790"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VN968_10915"
     CDS             complement(<1..527)
                     /locus_tag="VN968_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL29786.1"
                     /translation="MIRSIAIAATLGGLVAGVAAYAIYPSMRPSEPTPAEQAAAAPDD
                     WPICTMTVTMPDAEWAKLDPEFAAGKKAVVAEDWDGAIAVLKSAALRDPRNADIQNYI
                     GYAYRRLRQMGPAIGHYQQALMLNPRHRSAHEHLGEAALVLSDAATAEQHLAHLEDIC
                     LIGCEEYDDLKRAIAG"
     gene            complement(541..>2065)
                     /locus_tag="VN968_10920"
     CDS             complement(541..>2065)
                     /locus_tag="VN968_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29787.1"
                     /translation="ASGAFAQQQEEKLGTLSFPTSCDPKVQAEFERGVAMIHSYWFLV
                     ARRVFESVLQQDPGCAIAYWGIAMDLLGNSLGGPPSRENAKAAWVALEKAREIGAKTD
                     RERGWIDAIRAYYRDHDKTDVETRLRAYNSAMEQMAQRYPDDYEVQVFYALTLQASAS
                     KTDMSYGNQLKSVAILEKLFEQNPQHPGVSHFIIHAYDFPPLAERGIAAAKRYGGIAP
                     AVPHARHMPSHIYSMVGMWEESIASNTSAMEIQPDYYHAADFTVYAHLQLAQDVKAKA
                     LIDKAIATPPRGDRPSGFGNFVAKALMETRYLLDRGDWQGAAAMPMNTTTVPIADSLY
                     RFTRGLGMARSGDVVGAKQEIEAMKALRTALQRADQSYWADRTEEQMLAISAWIAAEE
                     GARDQAVRFMRAAADGEDGSIKNVIMENRLYPLREMLAELLLEVGQPAPALEEYETAL
                     KHTPNRYRAFFGAARASDAVGNRAQAAEYFGKLVNLAKNADAERQEVREAKAFLARR"
BASE COUNT          323 a          692 c          668 g          382 t
ORIGIN      
        1 cccgcgatcg cccgcttgag gtcgtcgtac tcctcgcagc cgatgaggca gatgtcttcc
       61 aaatgcgcga ggtgctgctc ggcggtggca gcgtcgctga gcaccagtgc ggcttcgccg
      121 agatgctcgt gggcactgcg atggcgcgga ttgagcatca gcgcctgctg gtaatgtccg
      181 atcgccggcc ccatctggcg cagccgccga taggcatagc caatgtagtt ctggatgtcg
      241 gcgttgcgcg gatcgcgtaa cgcggcggat ttcagcaccg caatagcacc atcccagtcc
      301 tcggcgacca ccgctttctt gccggcggcg aactcggggt cgagcttcgc ccactcggca
      361 tccggcatcg tgaccgtcat ggtacagatc ggccagtcgt cgggggcggc cgccgcctgc
      421 tcggccggcg tcggctcgct cggccgcatc gacggataga tcgcgtacgc ggccacgccc
      481 gcgaccagcc cgccgagcgt tgcagcgatg gcgatcgagc ggatcatgcc ggcgatccta
      541 tcacctccgc gccaggaacg ccttcgcctc ccgcacttcc tgccgctcgg catcggcgtt
      601 tttggcgagg tttacgagct tgccgaaata ctcggccgcc tgcgcacggt tgcccaccgc
      661 gtcggaggcg cgcgcggcgc cgaagaacgc gcggtagcgg ttgggtgtgt gcttgagcgc
      721 ggtctcgtat tcctcgagcg ccggcgcggg ctggccgact tccagcagca gttcggccag
      781 catctcgcgc agcggataga ggcggttttc catgatcacg ttcttgatgc tgccgtcctc
      841 gccatccgcg gccgcacgca tgaaccgcac cgcttggtcg cgcgcgcctt cctccgccgc
      901 gatccaggcg gaaatagcca gcatctgctc ctcggtgcga tcggcccagt aggactgatc
      961 ggcgcgttgc agcgccgtgc gcagcgcctt catggcctcg atctcctgct tggcgccaac
     1021 aacgtcgccg ctgcgcgcca tgccgaggcc gcgggtgaac cgatagagcg aatccgctat
     1081 cggcaccgtg gtggtgttca tcggcatcgc cgcagcgcct tgccaatcgc cgcggtcgag
     1141 cagatagcgc gtctccatca gcgccttcgc cacgaagttg ccgaagccgc tcggacgatc
     1201 gccgcgcggc ggcgttgcaa tcgccttgtc gatcagcgcc ttcgccttga cgtcctgcgc
     1261 cagttgcagg tgtgcataca cggtgaaatc ggcggcgtgg tagtagtcgg gctggatttc
     1321 catcgcggat gtattcgagg cgatcgactc ctcccacatc cccaccatcg aatagatgtg
     1381 cgatggcatg tgacgcgcgt gcgggaccgc cggcgcgatg cctccgtagc gcttggccgc
     1441 tgcgatgccg cgctccgcga gcggcggaaa gtcgtaggcg tgaatgatga agtgcgagac
     1501 gccaggatgc tgcgggttct gctcgaacag tttttccagg atggcgaccg acttgagctg
     1561 gtttccgtag ctcatgtcgg tcttcgaggc cgacgcctgc aaggtcagcg catagaaaac
     1621 ctgtacctcg tagtcgtcgg ggtagcgctg cgccatctgc tccatcgcgc tgttataggc
     1681 gcgcaggcgg gtctcgacgt cggtcttgtc atgatcgcgg tagtaagcgc gaatcgcgtc
     1741 gatccagccg cgctcgcgat ccgtcttcgc gccaatctcg cgcgccttct ccagcgcaac
     1801 ccaggccgct ttggcattct cgcgcgacgg cggcccgccc agcgagttgc cgagcaaatc
     1861 catcgcaatg ccccaatagg cgatggcgca accgggatcc tgctggagga cactctcgaa
     1921 cacgcggcgc gcgaccagga accagtagga atggatcatc gcgacaccgc gctcgaactc
     1981 cgcctgcacc ttcggatcgc aggaggtcgg gaagctcagc gtaccaagct tctcctcctg
     2041 ctgctgggcg aaggcgccgg aggcg
//
LOCUS       DATNOI010000281         6079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_951792,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000281 DATNOI010000000
VERSION     DATNOI010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951792"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VN968_10925"
     CDS             complement(<1..570)
                     /locus_tag="VN968_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HXL29788.1"
                     /translation="MALSSMTGFARSNGASGPYAFEWELKSVNAKGFDLRMRLPPGWD
                     DLEAFAKKRAGEVLSRGTVYANLNLKRANAVSTIRINEEVLASIVRVAGVLAGKIDAV
                     APSIDGLLAIKGVIEVVEPESDEAEDKAAKAAAAAAFEQALANLVEMRQREGVTLGQI
                     LSQRMDEIESLAKKAEAAPGRKPDAIRARL"
     gene            complement(753..2012)
                     /gene="mltG"
                     /locus_tag="VN968_10930"
     CDS             complement(753..2012)
                     /gene="mltG"
                     /locus_tag="VN968_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXL29789.1"
                     /translation="MSERPPISPRSPRAALEPERVPPPPKRSERARNPFVVVGNAIIT
                     IVLVLMIGAGGVYVYGKQKLEAPGPLQDDKVVNIPARAGMTDIADILQREGVIDNNRW
                     AFIGSVFALKARSELKPGEYSFQKNASLRDVIGTIVEGKVVQHSVTIPEGLTSEQIVT
                     RLSENDIFAGAVREMPREGTLLPETYKFPRGTTRDQVLQRMQQTQKRVLAEIWERRNP
                     DIPVKTPEQLVTLASIVEKETGKADERSRVAAVFVNRLRQKIKLQSDPTIIYGLVGGR
                     GTLGRPIKRSEIQQPSPYNTYVVEGLPPGPIANPGRASLEAAANPARTRDLFFVADGT
                     GGHAFTETYDQHQKNVAKLRTMEKQIQNDTAEPSDDLQPPAAGGPVDAAPTATTKPAP
                     PKKPARSAAPARQGAAQSTAAPPVVQQ"
     gene            complement(2144..3409)
                     /gene="fabF"
                     /locus_tag="VN968_10935"
     CDS             complement(2144..3409)
                     /gene="fabF"
                     /locus_tag="VN968_10935"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HXL29790.1"
                     /translation="MRRVVVTGLGMVSPLGCGVEPTWKRILNSESGAKKIDTFDVSDL
                     TSRIACVIPRGDGTGGTFNPDQWMEPKDQRKVDDFIIFAMCAARQALDDANWHPSTEE
                     ERCATGTMIGSGIGGLSGIADTALLLKERGPRKISPFFIPGRLINLASGYVSIEHGLK
                     GPNHAVVTACSTGAHAIGDAGRLIALGDADVMVAGGTESPICRLSMAGFCASRALSTG
                     FNDTPEKASRPYDRDRDGFVMGEGAGIVVLEEYEHAKKRGARMYAEVIGYGLSGDAYH
                     ITSPTPDGDGAFRSMSAAMKRAGITSSDIDYINAHGTSTQVGDEIELSAVERLLGNAA
                     SRVSMSSTKSSTGHLLGAAGAIECIFSVLAIRDNIAPPTINLDNPSMETAIDLVPQTP
                     RRREINVALSNSFGFGGTNASVIMQRVAN"
     gene            complement(3506..3745)
                     /locus_tag="VN968_10940"
     CDS             complement(3506..3745)
                     /locus_tag="VN968_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXL29791.1"
                     /translation="MSEIGERVKKIVVEHLGVEPEKVVDNASFIDDLGADSLDTVELV
                     MAFEEEFGCEIPDDAAETILTVGDATKFLEKNAKS"
     gene            complement(4047..4784)
                     /gene="fabG"
                     /locus_tag="VN968_10945"
     CDS             complement(4047..4784)
                     /gene="fabG"
                     /locus_tag="VN968_10945"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HXL29792.1"
                     /translation="MFDLTGRTALVTGATGGIGGAIAQALHAQGATVAISGTRREVLD
                     TLAGKLGERVHVLPCNLSDAAEVEALVPSAEQAMGQVDILVANAGITRDNLFVQLRDE
                     DWDEVIKVNLTATFRLARAATRLMMRKRFGRIIAITSIVGVTGNPGQGNYTASKAGLI
                     GMIKTLGAEYAKRNVTANCIAPGFIATPMTDALNDKQREAILSKVPAARLGTPEDIAA
                     AAVYLSSNEAAYVTGQTIHVNGGMAMI"
     gene            complement(4803..5762)
                     /gene="fabD"
                     /locus_tag="VN968_10950"
     CDS             complement(4803..5762)
                     /gene="fabD"
                     /locus_tag="VN968_10950"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HXL29793.1"
                     /translation="MTAAFTFPGQGSQAVGMGKALAEAFPAARAVFDEVDAALGEKLT
                     AVIWDGPAETLQLTENAQPALMAVSIATLRVLETEAGFSVGRDAAFVAGHSLGEYSAL
                     AAAGSLSISDTARLLRIRGLAMQKAVPVGVGAMAALLGLDYETAVGVANEAAQGQVCQ
                     AANDNGGGQVVVSGDRAAVERALEIAKTKGAKRAMLLPVSAPFHCRLMQPAADAMAKA
                     LADVSIRQPVSPLVSNVLAAPITDPDEIRRRLIEQVTGTVRWRESVAYMASQGVTRFF
                     EIGAGKVLSGLVKRIADGAIGISVGGPNDIASAKQALTAARAA"
BASE COUNT         1084 a         2006 c         1792 g         1197 t
ORIGIN      
        1 aagccgtgcc ctgatcgcgt cgggcttgcg gccgggagcg gcctcggcct tcttcgccag
       61 gctctcgatc tcgtccatgc gctgcgagag gatctgcccg agcgtcacgc cctcgcgctg
      121 gcgcatctcg acaagattcg ccagcgcctg ttcgaaggct gccgcggccg cagccttcgc
      181 agccttgtct tccgcctcat cgctttccgg ctcgacaacc tcgatgacac ccttgatcgc
      241 caaaagcccg tcgatgctcg gcgccaccgc atcgatcttg ccggcgagta cgcctgcgac
      301 ccttacgatc gaggccagca cttcctcgtt gatgcggatc gtcgagaccg cgttggcacg
      361 cttgaggttc agattggcgt acaccgtacc ccgcgacagc acctcgccgg cgcgcttctt
      421 ggcgaaggcc tcgagatcgt cccatccggg cggcagccgc atccgcagat caaacccctt
      481 ggcgttgacc gacttcaatt cccattcaaa tgcatatggt ccgctggcgc cgttacttcg
      541 ggcaaaacca gtcatgctcg acagcgccat cgcgtgaaat tctccgaaag ggacgggtgt
      601 gagattcgca agccagaaac tgccgcaacc ttagcgccga ttccgcaaaa gtggataccg
      661 gttttgcgaa cgagataagc gctaagttac tgcctggccg cgttttcgac ccatgtggaa
      721 ttcggttccg gcctgccgcc gccaagcctg cgctattgct ggaccaccgg aggcgccgcc
      781 gtcgactgtg ccgcgccctg ccgggccggg gcagcgctgc gggcaggctt tttcggcggc
      841 gccggctttg tcgtggccgt gggggcggcg tcgaccggcc cacccgccgc aggcggctgc
      901 agatcgtcgg aaggttcggc ggtgtcgttc tgaatctgct tttccatcgt gcgaagcttg
      961 gcgacgttct tctggtgctg atcgtaggtc tcggtaaagg catgaccgcc ggtgccgtcg
     1021 gcgacgaaga acagatcgcg ggtgcgcgcc ggattggccg cggcttccag cgaggcgcgc
     1081 ccgggattgg cgatcggccc tggcggaagg ccctcaacca cgtaggtgtt atagggcgac
     1141 ggctgctgaa tttcggatcg cttgatcggc cgacccagcg ttcccctgcc gccaaccagc
     1201 ccatagatga tggtcggatc ggactgcagc tttatctttt gccgcagccg gttgacgaac
     1261 accgcagcga cccggctgcg ttcgtccgcc ttgccggttt ccttctcgac gatcgaggcc
     1321 agcgtaacca gctgctccgg cgtcttgact ggaatgtccg gattgcggcg ttcccatatt
     1381 tcggccagca cccgcttctg cgtctgctgc atgcgctgaa gcacctgatc gcgcgtagtg
     1441 ccgcgcggaa atttgtaggt ctccggcagc aatgtaccct cgcgcggcat ttcgcggacg
     1501 gcgccggcaa aaatgtcgtt ctccgaaagc cgggtaacga tctgctcgga ggtcaggcct
     1561 tccggtatcg tgacggagtg ctggaccacc ttgccttcga cgatggtgcc aatgacatcg
     1621 cgcaggctgg cgttcttctg aaacgagtat tcgccgggct tgagctcaga gcgcgccttc
     1681 agcgcaaaaa cgctgccgat gaacgcccag cgattgttat cgatcacgcc ttcgcgctgc
     1741 aaaatatcgg caatgtcggt cattcccgcc cgcgccggaa tgttgacgac cttgtcgtcc
     1801 tgcaacggtc ccggggcctc gagcttctgt ttgccgtaga cgtatacacc gccggcgccg
     1861 atcattaaga ccagcacaat ggtaataatg gcattgccga cgacgacgaa cggattgcgg
     1921 gcgcgctcgg agcgcttcgg cggcggcggg acccgctcgg gctccagcgc ggcgcgcgga
     1981 ctccttggcg aaatgggcgg cctctcactc atcgatgcaa cctaaatcct gtcggttcaa
     2041 tcgcagcctc gcgaaacttc cgcgtcggcc gatagtctac gcccaattct taggattcat
     2101 caccgaatgc ggcgaatcgg tggactctac ttcatatgaa gcgtcagttg gcgacgcgct
     2161 gcatgattac ggatgcgttg gtgcccccga aaccgaacga attcgacagc gcgacattga
     2221 tttcgcgcct gcgcggcgtt tgcggcacaa gatcgatggc cgtttccatc gacgggttgt
     2281 ccagattgat ggttggcggc gcgatgttat cgcgaatcgc aagaacgctg aagatgcact
     2341 cgatcgctcc cgccgcaccc agcagatgtc ccgttgacga tttggttgac gacatcgaca
     2401 ccctggaagc ggcgttgccc aaaaggcgtt ccaccgcact gagctcgatt tcgtcaccga
     2461 cctgcgtcga ggttccgtgc gcattgatgt agtcgatatc ggaagaagtg atccctgcgc
     2521 gcttcatggc ggcgctcatg ctgcggaacg cgccatcacc atccggcgtc ggagatgtga
     2581 tgtgataggc gtcaccggat aggccgtagc cgatcacttc cgcatacatc ctcgcaccgc
     2641 gctttttggc gtgctcgtat tcctcaagca ccacgatgcc ggcaccttcc cccatcacaa
     2701 aaccgtcgcg atccctgtcg taggggcgcg atgctttttc cggggtatcg ttgaagccgg
     2761 tcgacagcgc gcgagacgcg caaaatccgg ccatcgatag ccggcaaatc ggggattcgg
     2821 ttccgccggc caccatcacg tctgcatcgc cgagcgcgat cagccggcct gcatcgccga
     2881 tcgcgtgagc gccggtcgaa caagcggtga ccaccgcatg gttggggccc ttgaggccgt
     2941 gctcgatcga cacataaccc gaggcaaggt tgatcagacg acccggaatg aagaatggtg
     3001 atatcttgcg cgggccgcgc tccttgagaa gcagcgccgt atcggcaata ccggacaggc
     3061 cgccgatccc cgagccgatc atcgtgccgg tggcgcaacg ctcctcttcg gtggaaggat
     3121 gccaattggc gtcatcgagc gcctgacgcg ccgcacacat tgcgaagatg atgaaatcgt
     3181 cgaccttgcg ctgatccttc ggctccatcc actgatcggg gttgaatgtg ccgccggtgc
     3241 catcgccacg cggaatcacg caggcgatcc ggctggtcag atcggagacg tcaaaggtgt
     3301 cgatcttctt ggcaccgctc tcgctgttga ggatgcgctt ccaggtcggc tccacgccac
     3361 agccgagtgg cgacaccatg ccgaggcccg tgacgacaac ccgcctcata tcagcactcc
     3421 agcccagaat tcgccgcgca caacaagaaa ccggtggacc gctcaaacac ggcccgtccg
     3481 gcttcgtcgg caccgcgcgg gggcgtcagc ttttcgcgtt cttttccaga aattttgtgg
     3541 cgtcgccgac ggtcaagatc gtctcggcgg catcgtcggg aatttcacaa ccaaactctt
     3601 cttcaaacgc catcacgagc tcgacggtat caaggctgtc ggcgccgagg tcgtcaatga
     3661 agctcgcgtt gtcgaccact ttttcgggtt caacaccaag gtgttcgacc acaatcttct
     3721 taactcgctc gccaatctca ctcatcgttt aacctcgtgc ttgttccatt ggacccgacc
     3781 ccaagacgat acgagccatc gtggtcgtta aactctcttc gcaatcgcgc atagtcttga
     3841 tttggccccg tcatagccga caaaaggccc cagtgaacga cgggcaggct ccgcaccgcc
     3901 aatatacagg gtttcaaatt cctgcaatgg cgttctttgc ctgtccgtta gtggttcggt
     3961 tatcatactt cccaagcctt gactataagc ctcaatcggt cctcggaaac ggcccttcgc
     4021 cccgccacgg ggcagtcgcg gccggctcaa atcatggcca ttccaccatt gacgtgaatt
     4081 gtctgcccgg tgacataagc cgcttcgttc gagctgagat agaccgccgc cgcagcgata
     4141 tcctcgggcg ttccgagccg cgccgcagga acttttgaaa gaatcgcttc acgctgcttg
     4201 tcgttaagtg cgtcggtcat cggcgtcgca ataaatcccg gggcgatgca attggcggtg
     4261 acgttgcgtt tggcgtactc ggcccccagc gtcttgatca tgccgatcag tccggccttc
     4321 gacgccgtgt aattgccctg ccccggattg ccggtgacac cgacgatcga cgtgatcgcg
     4381 atgatgcggc cgaagcgctt gcgcatcatc agcctggtcg cggcccgtgc caggcggaag
     4441 gtcgccgtca aattgacctt gatgacctcg tcccagtcct cgtcgcgcaa ttgcacgaac
     4501 agattgtcgc gggtgatgcc cgcgttggcg acgagaatgt cgacctggcc catcgcctgt
     4561 tctgcagacg gaaccagcgc ctcgacctcg gcggcgtcag agagattgca aggcagcacg
     4621 tgaacccgct cgcccagttt gcccgcgagc gtgtccagca cctcgcgccg cgttccggaa
     4681 atggccaccg tcgcgccttg cgcgtgcagc gcttgcgcga tcgcgccgcc gatgccgccg
     4741 gtcgcgccgg tgacgagcgc cgtcctgcca gtcagatcga acatcttggt ctccctcctc
     4801 gctcaggctg ctcgcgcggc agtgagcgcc tgtttcgccg aggcaatatc atttggcccg
     4861 ccgaccgaaa tcccaatcgc gccatcggca atacgcttga ccagcccgct gagcaccttg
     4921 ccggcgccga tttcgaaaaa ccgtgtgaca ccctggcttg ccatgtaggc caccgactca
     4981 cgccagcgca cggttcccgt aacctgttcg atcaggcggc ggcgaatctc gtcaggatcg
     5041 gtaatcggcg cggccagcac attcgatacc aatggcgaga ccggttgcct gatgcttaca
     5101 tcagccagcg ctttggccat cgcatcggcc gccggctgca tcagccggca atgaaacggc
     5161 gccgacaccg gcagcaacat cgcgcgcttg gcgcccttgg ttttggcaat ttccaacgca
     5221 cgttcgaccg cggccctgtc gccggagacc acaacctgcc cgccaccatt gtcattggcg
     5281 gcctggcaga cctggccctg cgcggcctcg ttcgcgaccc cgaccgcggt ctcataatcc
     5341 agaccgagca acgcggccat tgcgccgaca cccaccggca ccgccttctg catggcaagg
     5401 ccacggatgc gcaacagccg cgcggtatcg ctgatgctga ggctgccggc ggcggcgagc
     5461 gcggaatatt cgccgagcga atgtcccgca acgaacgcgg cgtcccgccc caccgagaaa
     5521 ccggcttcgg tctccagcac acgcagggtg gcgatcgata ccgccatcaa tgccggctgg
     5581 gcgttctcgg tgagctgcag ggtctcggcc gggccgtccc agatcacggc cgtgagcttt
     5641 tcgccgagcg cggcatcgac ctcatcaaac accgcgcggg cggccgggaa ggcctccgcc
     5701 aacgccttgc ccatgccgac cgcctgggag ccctgccccg gaaaagtgaa tgctgccgtc
     5761 atcggcgttc cctgaacgtc cctgaaatcc ctggagttgg gccgaaagac accgcaggag
     5821 cgggttgtgt caagccaccg ccggttccca gccaccgggc gccgccgccc gggccgctcg
     5881 ctttcccttg ccatcgcggt caaaatcctt ataaaccgcg ctatccgtgg atcccggccg
     5941 ggagctgaac ggacggtctc agcaatttca ccttatcggc gatgttgatg tggcagggcc
     6001 agtcggtccg tcgtcccgtg cttccgcctt ctgagcatat cgagtccttt ccgaagggtt
     6061 cgaagggctt gccgccggg
//
LOCUS       DATNOI010000282         3793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1262361,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000282 DATNOI010000000
VERSION     DATNOI010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262361"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VN968_10955"
     CDS             <1..340
                     /locus_tag="VN968_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXL29794.1"
                     /translation="ATQCDGVILMFEKMFESRMFFVDKLISMGARIVLCDPHRAIVAG
                     PSRLKGAPMTSPDIRAGMAMLLAAVCAKGVSTIDNADQIERGYERIDERLNALGARIT
                     RVPERTPDAG"
     gene            403..1836
                     /locus_tag="VN968_10960"
     CDS             403..1836
                     /locus_tag="VN968_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXL29795.1"
                     /translation="MTSLEKIERGTVTAPHVAAAPGSRLARELTETLRLAVPIALTQL
                     GQIAMMTTDLALVGRLGDEAVAAAALAHTVAFISFAFGMGLVSAVAPLAAQAFGARNP
                     HRVRRALRVGLWAALLISLPMLAFRLCGEQILLMLGQAPATAHLAQRYLLGLAWGITP
                     ALWFLAIRGFMGAVNRPEPGLWITLVAIPANAVLVYLLIHGKLGLPRLELFGAGLATT
                     IINFGMFLASLWFAYRRPPFRKYQVLGRIWRVDWMLMRQLIVIGAPISISFLLEYGLF
                     AAAALLMGLISTTALAAHQIALQVTAILFMVPLGIGIAATVRVGHAVGRNDAPAVKRA
                     GFVATLLGIVLVSILTLAVILTRFTIARFFFGAAVESGGAVIELTATLLMVGATFFVA
                     DGIQTVAAGALRGMNDTRMPLLFAAISYWLIGFTSAYGLAFWTDLGAVGVWIGLSCGT
                     AVYAVLLILRFRRLAGHLGTARIVGAN"
     gene            1860..2327
                     /locus_tag="VN968_10965"
     CDS             1860..2327
                     /locus_tag="VN968_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HXL29796.1"
                     /translation="MKVSEVDPDVDTGVLLAGAQFVDAYSIAIDNGALDARQAAEQMM
                     GRSPRWVEALLKLRNFLVTPFGLKTSAPSEASDVIGLFPVLSQTPDRLIAGFNDSHLD
                     FRVVVDVVRSGSSQSVTATTLVLTHNWLGRAYLAIILPFHRLIVPSMLRQVAA"
     gene            2404..3057
                     /locus_tag="VN968_10970"
     CDS             2404..3057
                     /locus_tag="VN968_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HXL29797.1"
                     /translation="MTLSVDLFFSFRSPYSYLALPKTLKLVADYDATVNLRPVYPLAV
                     RVPGFFKRTNPQFLRYVGLDSRRVAEHANIPFRFPRPDPIVQDLTTLDVAEHQPYIHR
                     LTRLGAAAQVEARALAFVDAISRVLWDGTVSGWNEGDHLARAADAAGFDLAAMDAAIS
                     ADPDRYERVIADNEKDHAASGHWGVPTFVFDNEPFFGQDRIDLLIWRMESKGLTRRR"
     gene            complement(3077..>3793)
                     /locus_tag="VN968_10975"
     CDS             complement(3077..>3793)
                     /locus_tag="VN968_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HXL29798.1"
                     /translation="VNPLDSAVDRVLEVTVGAVTGLLVSFFVLPSRAHNQIRVSAARS
                     LELIAAALSELLAGLTRGRDNDALHQIQDGIGAALVGLNATGAEAERERAAHLSSGPD
                     TGPLLRTILRLRHDVVMIGRASVVPLPSELQARLAAPLAKVGDAIASYLRSTAAALRS
                     GVGAPAIWPMHEALQGYAAEIASLRSEGLTRGLPGDVAERVFALGFSLEQMRQNLKDL
                     ERCVAEWSETPSVATTREED"
BASE COUNT          601 a         1244 c         1219 g          729 t
ORIGIN      
        1 ggcgacccag tgcgatggtg tgatcctgat gttcgagaag atgttcgagt cgcggatgtt
       61 cttcgtcgac aagctgatct cgatgggcgc ccgcatcgtg ctgtgcgatc cgcaccgggc
      121 gatcgtggcc gggccgagcc ggctcaaggg cgcgccgatg acatcccccg acatccgcgc
      181 cggcatggcc atgctgctgg ccgcggtctg cgccaagggc gtcagcacca tcgacaacgc
      241 cgaccagatc gaacgcggct atgagcgcat cgatgaacgg ctcaacgcgc tgggcgcgcg
      301 gatcacccgc gtgccggagc ggacgcccga cgccgggtag aagaaatggg ctggtaccgt
      361 gccgaccacc atttttgtcg gtggaggtga taatctgagc ccatgacatc ccttgaaaaa
      421 atcgagcgag gaacggtcac cgcgcctcat gtagcggcag cgccaggcag tcggctcgcg
      481 cgcgagctga ccgagacgct caggctggcg gtccccatcg cgctgacgca actcgggcag
      541 atcgcaatga tgaccaccga tcttgccttg gtcggacgtc tcggggatga agccgttgcc
      601 gcggcggcgc tggcgcatac ggtcgccttc atcagcttcg cgttcggaat gggcctggtg
      661 tcggccgtgg cgccactggc cgctcaagcg tttggcgccc gcaacccgca ccgggtgcgg
      721 cgcgcgcttc gcgtcggcct ttgggctgcg ctgctgattt ccctgccgat gttggcgttt
      781 cgattgtgcg gcgagcaaat cctgctgatg ctcggccagg ctcccgcgac cgcccacctc
      841 gcgcagcgat atctgctggg cctggcgtgg ggcattacgc cggcgctgtg gtttctcgcg
      901 atccgcggct tcatgggcgc ggtcaaccgg ccagagccgg gactgtggat cacgcttgtc
      961 gcgatcccgg ccaatgccgt gctggtctat ctcttgatcc atggcaagtt gggattgccg
     1021 cggctggagc tgttcggcgc cggcctcgcc accacgatca tcaatttcgg gatgtttctg
     1081 gcgagcctct ggtttgccta tcgccgcccg ccattcagga aatatcaggt gctgggtcgc
     1141 atctggcgcg tcgactggat gctgatgcgg caactgatcg tcatcggcgc tccgatctcg
     1201 atctcgttcc tgctggaata cggcctgttc gccgccgcgg cgctgctgat gggcctgatc
     1261 agcaccaccg cgctggccgc acatcagatc gctttgcagg ttacggccat cctgttcatg
     1321 gtgccgctcg gcatcgggat cgcggccacc gtgcgggtcg ggcacgccgt cggccgcaac
     1381 gatgcgcccg cggtcaagcg cgcgggcttc gttgcgacgt tactcggaat tgtcctcgta
     1441 tccatcctga ccctcgcggt gatcctcacc cggtttacga tcgcgcgatt tttcttcggc
     1501 gcagccgtcg aaagcggagg cgccgtcatc gaactcaccg cgacgctgct gatggtcggc
     1561 gcgaccttct ttgtcgccga cggcatccag acggtcgcgg ccggggcgct gcgcggcatg
     1621 aacgacacgc ggatgccgct gctgttcgcc gcgatcagtt actggctgat cggatttact
     1681 tccgcctacg ggctcgcgtt ctggacggat ctcggcgcgg tcggggtctg gatcgggctg
     1741 tcctgcggca ccgccgttta tgccgttctt ctgatcttgc gtttccggcg gcttgccggt
     1801 catctgggga ccgcacggat cgttggtgcg aattgatccg caaccgagag gggttggtga
     1861 tgaaagttag cgaggtcgat cctgacgtcg acaccggcgt gttgctggcc ggcgcgcaat
     1921 ttgtcgatgc ctacagcatc gcgatcgaca atggcgcgct cgatgcccgg caggcggctg
     1981 aacagatgat gggacgctcg ccgcgctggg tcgaagcgct gctgaaattg cgcaactttc
     2041 tggtaacgcc ctttgggctg aaaacatccg cgccgagcga ggcctccgac gtcatcgggt
     2101 tatttccggt tctcagccaa acgcccgacc gcctgatcgc gggtttcaac gacagccatt
     2161 tggattttcg cgtcgtggtg gacgtggtga ggtcgggcag cagccagagc gtcacggcga
     2221 ccactttggt gctgacgcac aattggctcg gccgggccta tctcgcgatc atcctgccgt
     2281 ttcaccggct gatcgtcccc agcatgctgc ggcaagtggc ggcctgaggg cgcgccgacc
     2341 cctcgtggac atcgatcccg acgcatgctt tgttccggcc ggacaatgcc ggccgaggca
     2401 ctcatgactt tatccgtcga tctgttcttt tccttccgca gcccctacag ctatctcgcg
     2461 ttgcctaaaa cactcaagct ggtggccgac tacgacgcga cggtaaacct tcgcccggtc
     2521 tacccgctgg cggtgcgcgt gcccgggttt ttcaagcgca ccaacccgca gtttctgcgt
     2581 tatgtcgggc tcgactctcg ccgcgtcgcc gagcacgcga acattccctt ccgctttccc
     2641 cgccccgacc ccatcgtgca ggacctgacg acgctcgatg tcgcggagca ccagccctac
     2701 atccaccggc tgacccggct cggcgccgcc gcgcaggttg aggcgcgcgc gctggccttt
     2761 gtcgatgcga tcagccgggt gctgtgggac ggcactgtca gtggctggaa cgaaggcgat
     2821 catctcgcca gggccgccga tgcagccggc ttcgatttgg ccgcgatgga tgcagcgata
     2881 tcggccgatc ccgatcgtta tgaacgggta atcgccgaca acgaaaagga ccacgcggcc
     2941 tccggccatt ggggtgtgcc gacgttcgtg ttcgacaacg agccgttctt cggccaggac
     3001 cgcatcgacc tgctgatctg gcggatggaa agcaaggggc tgacccgccg tcgctgatgt
     3061 cgacggcagg acaggattag tcctcttcgc gggttgtcgc caccgatggc gtttcggacc
     3121 actcggcgac gcagcgctcg aggtctttca gattttgccg catttgctcc agcgaaaatc
     3181 ccagcgcaaa aacccgctcg gcgacgtcgc cgggcaggcc acgcgtcagg ccttcgctgc
     3241 gcaagcttgc gatttccgcg gcgtagccct gcagcgcctc atgcattggc cagatcgcgg
     3301 gagcgccgac accgcttcgc aatgccgcgg ccgtggaacg cagatagctg gcgatcgcgt
     3361 cgccgacttt ggccaagggt gctgcgagcc gggcctgcag ttccgacggc aacggcacca
     3421 cgctcgcccg cccgatcatc acgacgtcgt ggcgtagccg caaaatggtc cgcagcagcg
     3481 gaccggtgtc aggccctgac gacagatgcg ccgcacgctc gcgctccgct tccgcgccgg
     3541 tcgcattcag gccgaccagc gctgcgccga tgccgtcctg aatctgatgc aaggcgtcgt
     3601 tgtcgcgccc gcgcgtcagc cccgccagca attcggagag cgccgccgca atcaattcaa
     3661 gcgaccgtgc ggcgctgacg cgaatctgat tgtgcgcccg cgacggcagc acgaagaaag
     3721 acaccaatag cccggtgacc gcgccaaccg tgacctcgag aacgcgatcg accgcggaat
     3781 cgaggggatt gac
//
LOCUS       DATNOI010000283         2633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1142391,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000283 DATNOI010000000
VERSION     DATNOI010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142391"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VN968_10980"
     CDS             complement(<1..240)
                     /locus_tag="VN968_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXL29799.1"
                     /translation="MWIVGLALRRPYTFIVLALLIALLGVFTILRTPTDIFPNIKIPV
                     VATVWRYSGLSPDEMATRLVLGSERATQVTVNGVEH"
     gene            complement(323..2353)
                     /locus_tag="VN968_10985"
     CDS             complement(323..2353)
                     /locus_tag="VN968_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL29800.1"
                     /translation="MSADADALAVENLEVAYRVRGRDLNVLRDLSFRIGSGEAYGLVG
                     ESGCGKSTVALAVVRYLPRNGRLLGGRILIGERDLYALRDEEMRRLRATTISMVYQDP
                     ARALNPSIRVGEQIAEIFRLRRLTAAQAKERAAAMLERVRIADGRSVYDRYPHQISGG
                     MQQRVAIAMALSINPKVLILDEPTTGLDATVEAAILDLVGQLRIELGTSILFISHDLA
                     VIARMCDRVGVLYAGALIEEGPTAEIFSNPRHPYTVGLLRCVPRHGRRKDHGRLDSIP
                     GNPPTPDDGIHGCMFAPRCGLAGEKCVTDAPPFFDLGNRLSRCHFHERAQALPHITPD
                     AAPTAITVDRSRPPILQAIGVSKTFGSARHRVRVLHAVSLQLWPGETLGLVGESGSGK
                     TTLARLLLGLEAPDPGGVVELGGIALAGRSETRRPSQLKALQIVFQNPDSALNRSHSI
                     RRLIERVLARLGRVPKPERAARLLELIRSVRLPDHYLSLRPRQLSGGLKQRVAIARAF
                     AGDPRIVVCDEPTSALDLSVQAAILNLLADLQAKHQVSYLFISHDLGSVRYLSDRIAV
                     LYLGRLMEIGPAERVFSGPHHPYTAALLSAVPSLGRQIEPRVHLEGEIPSAIHVPNGC
                     VFHTRCPRKLGPICEEQQPPLHPGAEPGHQIRCHIRAADLGGTDRLDRSRGP"
     gene            complement(2350..>2633)
                     /locus_tag="VN968_10990"
     CDS             complement(2350..>2633)
                     /locus_tag="VN968_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL29801.1"
                     /translation="YILFAEILPNVIPPIVVEAGVRLGYAIFTVATLSFIGFGIQPPS
                     PDWGLAVSESYGLINGGIWWPVLFNALAIASLVIGVNLAADGIQAAVDA"
BASE COUNT          511 a          812 c          859 g          451 t
ORIGIN      
        1 gtgctcgacg ccattgaccg tcacttgtgt tgcgcgctcg gaccccagga ccaggcgcgt
       61 ggccatctca tccggcgaga ggcccgagta gcgccatacg gtcgcaacca ccggaatttt
      121 gatgttgggg aatatgtcgg tgggcgtgcg caggatcgtg aacaccccga gcagcgcaat
      181 caggagcgca agcacgatga acgtataggg gcgccgcagc gccagaccaa cgatccacat
      241 gtaccctcac agcgttccgc caacttcgaa cactgccagt cccgatgaga cagtgatcat
      301 gataccgcat cccaaagtgc ttttacggcc cgcgactgcg atcgagccgg tctgtaccgc
      361 cgagatctgc cgcgcgaata tggcaacgaa tctgatggcc tggctcggcg ccgggatgga
      421 gcgggggctg ctgctcctcg caaatgggac ccaatttgcg gggacaacgc gtgtggaaga
      481 cacagccgtt cggcacatgg atggcgctgg ggatctcgcc ctccaggtgc acacgcggct
      541 cgatctggcg accgaggctc gggactgcgg agagcaaagc cgccgtatag ggatgatggg
      601 gccctgagaa gactcgctcg gccggaccga tttccatcaa ccggccgaga tagagcactg
      661 cgatccggtc ggatagatag cgcacgctgc cgaggtcgtg cgaaatgaac agataactga
      721 cctgatgctt cgcctgtagg tcggccagca ggttcagaat cgccgcttgc accgataggt
      781 cgagcgcaga ggtcggttca tcgcaaacca cgatgcgtgg atcgccggca aaggctcgcg
      841 caatcgcgac gcgctgcttc aatccgcccg atagctggcg agggcgtaga ctcaaatagt
      901 ggtcgggcag gcggaccgat cggatcaatt cgagtagccg cgcggcccgt tccggtttgg
      961 gcactcgccc cagcctggca agaacgcgtt cgatcaggcg gcgtatgctg tgcgatcggt
     1021 tcaacgccga atccgggttc tgaaagacga tctgcaatgc tttcagttgg cttggcctgc
     1081 gcgtctcgct ccggccggcg agcgctatgc cgcccaattc aacgactccg ccgggatcgg
     1141 gcgcctcgag acccagcaga aggcgcgcga gcgtggtctt gccgctcccc gattccccaa
     1201 ccaggccgag cgtttccccg ggccacagct gcagcgagac ggcgtgcagg acgcgcactc
     1261 gatggcgtgc gctaccgaag gttttgctca cgccgatggc ctgcaggatc ggcgggcggc
     1321 tgcgatcgac ggtgattgcc gtcggcgcag catcgggtgt gatgtggggt agcgcctgcg
     1381 cccgctcgtg aaaatggcag cggctgaggc ggttgcccaa atcgaagaac ggcggcgcgt
     1441 cggtcacgca tttttcgccg gccaatccgc accgcggggc gaacatgcag ccgtgaatgc
     1501 cgtcgtccgg cgtcggcgga ttacccggaa tgctgtcgag ccggccgtga tccttgcgcc
     1561 gcccgtgccg tggcacgcag cgcaacagac cgaccgtgta ggggtgccgc ggattactga
     1621 agatttcggc cgttggacct tcttcaatca aggcgccggc atagagcacg cccacgcgat
     1681 cgcacattct ggcaataacg gcgaggtcgt ggctgatgaa cagaatggac gttccgagct
     1741 cgatgcgcag ctggccgacc agatcgagaa tcgcggcctc caccgtcgca tcgaggccgg
     1801 tcgtcggttc gtcgagaatg agcactttgg gattgatcga cagcgccatg gcgatcgcta
     1861 cgcgctgctg catgccgccg gatatctggt gcggatatcg atcgtagacc gaacgaccgt
     1921 cggcgatccg cacgcgttcc agcatggcgg cggccctttc cttcgcctgc gcggccgtca
     1981 ggcgtcgtag tcggaagatt tcggcgatct gctcgcccac tcgaatcgat ggattcaggg
     2041 cacgggccgg atcctgatag accattgaaa tcgtcgtggc gcgcaggcgg cgcatttctt
     2101 cgtcgcggag cgcgtagaga tcacgctcgc cgatcaggat cctgccgccc aagaggcggc
     2161 cgttacgcgg cagatagcgc accacagcga gcgccaccgt cgatttaccg cagccggatt
     2221 cgccgaccag cccataggct tcgcccgagc cgatgcgaaa gctaaggtct cgcagcacgt
     2281 tgagatctcg gccgcggaca cggtaggcga cttcgaggtt ttcgacggca agcgcatccg
     2341 catcggcgct catgcgtcga cggcggcctg gatgccgtcc gcggccagat tgacgccgat
     2401 gaccagcgag gcaatcgcga gtgcgttgaa cagcacgggc caccagatgc cgccattgat
     2461 caagccgtaa ctttcggaaa ccgcgagccc ccaatccggc gacggcggct ggatgccgaa
     2521 tccgatgaag ctcagcgtcg ccacagtgaa gatggcgtag cccaaacgaa cgccggcttc
     2581 gaccacgatg ggtggaatga cattggggag gatttcggcg aacaatatgt aga
//
LOCUS       DATNOI010000284         3102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2221636,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000284 DATNOI010000000
VERSION     DATNOI010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221636"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VN968_10995"
     CDS             complement(<1..284)
                     /locus_tag="VN968_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29802.1"
                     /translation="MANERNPDDLYRRNPADDEYRWPPHLDNELQPDPELAEGPASGA
                     KVAMFAVAIAVVLGAIFYGLNNTTINQAGTSSTAQNSSQTSPAAAPRTNTG"
     gene            complement(399..1625)
                     /locus_tag="VN968_11000"
     CDS             complement(399..1625)
                     /locus_tag="VN968_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXL29803.1"
                     /translation="MMQGRHHAIRAILALVLLCTAFSGKAQGQPSADTIGRGKALVEA
                     ADCASCHSADPSKPFAGGKRIDTPFGAIYSPNLTPDRETGLGAWSDDEFSRALRYGVR
                     RDGSRYYPVFPYPNFTKLTRDDLLAIRAYLATLTPVSNSPPPPELRWPLNYRVIMRAW
                     NFLFFRPGVFEPNQQKTTEWNRGAYLVTGAAHCGACHTPKNLFGADRSGRAFGGGLVQ
                     GWFAPRLDGAERGGLKSWSVADIVEYLQSGRNARSHAGGLMAEVVANSTSKMSDADIR
                     AIAVYLKDLPAGKSDPVVTPPPAAEMAAGKAIYAHACVACHETDGSGAPRIYPPLPGN
                     ANLQSVDPSSALRIILDGAQTLTTPRAPNPGSMPAYAKQLSDQEIADVTNYMRNSWGN
                     AAPLVTPAQVKKARKQ"
     gene            1880..>3102
                     /locus_tag="VN968_11005"
     CDS             1880..>3102
                     /locus_tag="VN968_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HXL29804.1"
                     /translation="MGINQGPISLDQKYTQGSGHIFLTGIQALVRLPMAQIRRDRAAG
                     LNTAGFISGYRGSPLGGYDQQLFAARKHLEQYNIKFQPGVNEDLAATAIWGSQQLNLS
                     PGARYDGVVGIWYGKGPGVDRCGDVFRHGNTAGSAKNGGVLCLAGDDHGAKSSTVPHQ
                     SDHAFISALMPYLYPSSIHEMIEMGLLGIAMSRYSGCWVGMKVITETVETTAEIDLSD
                     EMTPFVIPTDFEMPPGGLNLRWPDDRYLQDLRLQDYKGFAAIAFARANKINRVTMDSP
                     NARFGIMASGKSYEDIRQALRELGITPEVAAKIGLRLYKIGMPWPLEPEGVRQFAVGL
                     EEIFIVEERREIVENQVKQELFNWRDDVRPRIVGKMDEHDKRFLTFAAELSVASLAGS
                     LTERLLRLNLNPEIAA"
BASE COUNT          565 a          963 c         1002 g          572 t
ORIGIN      
        1 ccggtgttgg tgcgaggggc tgcagcaggc gacgtctgcg agctgttctg ggcggtcgag
       61 gacgtgccgg cctgattgat cgtcgtgttg ttcaggccgt agaaaatcgc gccaagcacg
      121 accgcgatgg cgacggcgaa cattgcgacc ttggccccgc tggcgggacc ctcggcgagc
      181 tctggatcgg gctgcaactc attgtcgaga tggggcggcc agcggtattc gtcgtcggcg
      241 ggattgcgac gatagagatc gtccggattg cgttcattgg ccatgattgc gttcctccgt
      301 tatctcctac caatggctgg gattgggcgg ggttccaagg gaacttggag gaaccggagc
      361 gtcattccgc gtaaacatca ttgacgttgc gcccgcgctt actgcttccg cgctttcttc
      421 acctgcgccg gcgtcaccag cggcgcggca ttgccccagg aatttcgcat gtagttcgtg
      481 acatcggcga tctcctgatc cgacaattgt ttggcgtaag ccggcatcga tcccggattg
      541 ggtgcgcgcg gcgtcgtcag ggtctgtgca ccgtcgagga tgatacgcag cgcgctggag
      601 gggtcgaccg actgcaaatt ggcattgccg ggtagcggcg gatagatccg cggggcgccg
      661 ctgccatcgg tttcgtggca ggcgacacag gcgtgggcgt agatcgcttt acccgccgcc
      721 atctcggctg ccggcggcgg ggtcaccacg gggtcagact ttcccgccgg aaggtccttg
      781 agatagaccg cgatggcgcg aatgtcggcg tcgctcatct tcgaggtgga attggccaca
      841 acctcggcca tcagtccgcc ggcatggctg cgcgcgttgc ggccgctctg cagatattcg
      901 acgatgtcgg cgacgctcca tgatttcagc ccgccgcgct ccgcgccgtc gagccgcggc
      961 gcgaaccaac cctgcaccag cccgccgcca aaggcgcggc cgctcctgtc ggcgccgaac
     1021 aggttcttgg gcgtgtggca ggcgccgcaa tgcgccgcgc ccgtgacgag ataagcgccg
     1081 cgattccatt ccgtggtttt ctgctggttc ggttcgaata cgccgggccg gaagaacagg
     1141 aaattccagg cgcgcatgat gacgcggtaa tttagcggcc agcgcagttc cggcggcggc
     1201 ggactgttgg aaaccggcgt cagcgtcgcg agataggcgc ggatcgccag cagatcgtcg
     1261 cgggtcaatt tcgtgaagtt gggataggga aaaaccgggt aatagcgcga accgtcgcgc
     1321 cgcacgccgt aacgcagcgc gcgggagaat tcgtcgtcgc tccatgcacc gaggccggtt
     1381 tcgcgatccg gcgtcaggtt gggcgaataa atcgccccga acggggtatc tatgcgcttg
     1441 ccgccggcga acggctttga cggatcggcg ctatggcaac tcgcgcaatc ggccgcttcg
     1501 acaagcgcct tgccgcgccc gatggtgtcc gccgagggct ggccctgcgc tttgccgctg
     1561 aatgcggtgc acaaaagcac gagcgcgaga atcgcccgga tggcgtgatg tcgcccctgc
     1621 atcatggatc gctccctggc ggcattataa cgcgaaacca tgtcggccag agcgccacgt
     1681 cgtttcgccc caggcccatc cggcgacaca aagcgaaatg gccggcgccc tttctcgcat
     1741 gaaattgcga tttgcgaatg cggggctcca atacccagat ttgtggtgcg cagccggcga
     1801 aaaccgacat agactggatt tggcgatcgg catgattagc taaaaaagag ccgtcggagc
     1861 caaggcttaa gagggtcgaa tgggaatcaa ccaaggtccg atcagtctcg atcagaaata
     1921 cacccaaggg tccggccata tcttcctgac ggggattcag gcgctggtcc gcctgccgat
     1981 ggctcagatc cgccgcgacc gcgccgccgg tctcaatacc gcgggcttca tctccggcta
     2041 tcgcggttct ccgctcggcg gctacgacca gcaattattc gcagcccgca agcacctcga
     2101 acagtacaac atcaaattcc agcccggcgt gaacgaagac ctggcggcca ccgcgatctg
     2161 gggttcccaa cagctcaatc tttcccccgg cgccaggtac gacggcgtcg tcggcatctg
     2221 gtacggcaag ggacccgggg tcgatcgctg cggcgacgtg ttccgtcacg gcaacacagc
     2281 gggctcggcc aagaatggcg gcgtgctctg tctcgccggc gacgaccacg gcgcaaagtc
     2341 ttccaccgtg ccgcatcagt ccgatcacgc cttcatctca gccctgatgc cctatctgta
     2401 tccttccagc atccatgaaa tgatcgagat gggcctgctc ggcatcgcga tgtcgcgcta
     2461 ctcgggctgc tgggtcggca tgaaggtgat caccgagacg gtggaaacca cagccgagat
     2521 cgacctcagc gacgagatga cgccgttcgt cattccgacc gatttcgaga tgccgccggg
     2581 cgggctcaat ttgcggtggc ccgatgaccg ttacctacag gatctgcgcc tgcaggacta
     2641 caagggcttt gccgcgatcg cgtttgcccg cgccaacaag atcaaccgcg tcaccatgga
     2701 ttcgccgaac gcacgcttcg gcatcatggc gtccggcaag agctacgagg atatccgcca
     2761 ggcgctgcgc gaacttggca tcacgccgga agtcgcggcc aagatcggtt tgcggcttta
     2821 caagatcggc atgccgtggc cgctggagcc ggaaggtgtc cggcaattcg cggtcgggct
     2881 cgaggagatc ttcatcgtcg aggagcggcg cgagatcgtc gagaaccagg tcaagcagga
     2941 gctgttcaac tggcgcgacg acgtgcggcc gcgcatcgtc ggcaagatgg acgagcacga
     3001 caagcgcttc ctgacctttg cggcggaact cagcgtggcc tcgctcgcgg gctcgctgac
     3061 cgagcggctg ctccgcctca atctcaatcc cgagatcgcg gc
//
LOCUS       DATNOI010000285        11703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2347320,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000285 DATNOI010000000
VERSION     DATNOI010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11703
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2347320"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VN968_11010"
     CDS             <1..226
                     /locus_tag="VN968_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29805.1"
                     /translation="LVAGCTCNGKDQVGLAPVRIENDPTLRKGDIVAGSDGLVVAGRG
                     ADKRGASLNFSPASLAVRARYQRVPVLAAE"
     gene            complement(369..2462)
                     /locus_tag="VN968_11015"
     CDS             complement(369..2462)
                     /locus_tag="VN968_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL29806.1"
                     /translation="MLNRLTVSALLKTVILTTAFCVVLGFSLNAWESWGRLQLTNRIS
                     VIADASANLFKAMHNLRTDRSTTTRLLNSDQPVDSDIDKYLRDLRDTEMPAMRNALAL
                     LPSIEFAQQQTLIPELDRLFKTLTAQQKEFWDEMSKPKASRRPALPKEYMETTAALLG
                     TLDKLSAMLAAAVNHQDPVIDQLLAIKQIAWLLRNTAGEASLLVSNAITAGQLLPETH
                     LKYVKFVGGTEIAWNALELTASGTQLPPALSAVMAATKTAYFEPDYLALRDRLLTTLM
                     AGGKPELTANQWTPITVGRLSSAVRVAEAALEAAKDHTSSQHSAALRSLVLQLVLLAS
                     ALGLTFGAMMAVSRRVIKPLHNMRDAMLKVASGDLTVDTGYAARRDEIGALAGALDTF
                     KQQAADKIRIETQERERNAGAATRQQAIEAYVSEFESTVRQTLDQLNDASGQMRNTSA
                     GLSTVSRQTNARVQTAEKASGEASLSVESVASASEELNASINDISQQAAHAAGIASRA
                     VNQARQTDGTVQGLAKTASRIGEVVGLINTIASQTNLLALNATIEAARAGEAGRGFAV
                     VASEVKSLASQTAKATEDISEQIADIQKVAGEAIDAIKGIGSIIGEVNEVATAIAAAV
                     QQQGTATQEIARSTQIASQGTRNVSDNITGVKADADAAAAAADDVKRASGKLETQSQH
                     LGNQVTQFLVKIRAA"
     gene            2686..2841
                     /locus_tag="VN968_11020"
     CDS             2686..2841
                     /locus_tag="VN968_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Thivi_2564 family membrane protein"
                     /protein_id="HXL29807.1"
                     /translation="MLVSALITFLVVILILYLINLLPIDGRGKQIARVVVIILGVISL
                     LKYIAVF"
     gene            complement(2907..3107)
                     /locus_tag="VN968_11025"
     CDS             complement(2907..3107)
                     /locus_tag="VN968_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HXL29808.1"
                     /translation="MDWNRVEGNWKQFKGKAKEKWGKLTDDDLNVIEGRREQLEGKLQ
                     QRYGFAKDQIHRDVDDWFGTLK"
     gene            complement(3184..5346)
                     /locus_tag="VN968_11030"
     CDS             complement(3184..5346)
                     /locus_tag="VN968_11030"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HXL29809.1"
                     /translation="MNRVDAHGLKIAPVLFDFIAKEATPKTGTSPDAFWAGLAAIIQN
                     LAPKNQELLAVRDSLQAKIDGWHRANKGKPFDMNGYTAFLKEIGYLLPEPATQKVETS
                     NVDEEIGKICGPQLVVPLTNARYALNAANARWGSLYDALYGTDAIPHDAGESGKGYNK
                     TRGDKVIAKAKAFLDAAVPLATGSHTDVTSYSVIAGQLSVKLKSGNATALKSNTQFAG
                     YQGDAAAPTAILLVNNGMHVEIKIDRGHMIGKDDPAGVADMILESAVSTILDMEDSVA
                     AVDAEDKVLIYRNTLGLMNGTLSADFEKGGKTLTRALNPDRVYKTSDGKELKLHGRSL
                     LLMRNVGHHMFTDAVLDAAGAEIPEGILDAAVAGLLAIHDLKGGSKTRNSRTGSVYIV
                     KPKMHGPDEVALTCELFGEVEKLLSLPENTMKVGIMDEERRTTVNLKACIQNASKRIV
                     FINTGFLDRTGDEIHTSMEAGPMIRKNDMKAQPWIKAYEDWNVDTGLIDGLPGHAQIG
                     KGMWAAPDKMADMLTQKIAHPQAGATTAWVPSPTAATLHALHYHQVNVQARQQELAKG
                     GPRAKLSDILTIPVSQSNWAPDHVKQEIDNNCQGILGYVVRWIDQGVGCSKVPDIYDV
                     GLMEDRATLRISSQHLANWLHQGVITGEQVMESLKRMAVVVDGQNKGDPLYRPMAPGL
                     DGVAFKAACDLIFKGREQPNGYTEFILTARRREAKAVG"
     gene            complement(5599..7524)
                     /gene="cysC"
                     /locus_tag="VN968_11035"
     CDS             complement(5599..7524)
                     /gene="cysC"
                     /locus_tag="VN968_11035"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HXL29810.1"
                     /translation="MNMMVPSATSLISATPNGTTRPQVRIVIVGHVDHGKSTLVGRLL
                     HETGSLPEGKLEMLKAVSARRGMPFEWSFLLDALQTERDQGITIDTTQISFRTRSRDV
                     VLIDAPGHAEFLRNMITGASQADGALLIIDALEGVRDQTRRHGYLLHLLGVKQVAVVV
                     NKMDRVDFGAARFAEIRDEISAHLIGLGLQPSAVIPISARDGDGVAERTPRTQWYNGP
                     TVIEALDALEPARPLETLALRLPVQAIYKFDDRRIVAGRIESGRLSAGDEIVIMPAGK
                     IAKIKTVEGWPVTPVTGSQSAGRSVGITLDRELFIERGDVIAHVGDSPRDTRRIRARI
                     FWLHDKPLVSGASILVRLGTRETRATVVAIEKAVDPGELSSIETKSIARNHVGEIDIS
                     LAQAIAADPYTDNPRTGRLVIEVNGRIAGGGLVLSVDAGQRAVPVDIVPVESALRPDE
                     RSARYRHGGAVVWLTGLPGSGKSTLARALERRLFSQGGSPVLLDGDTLRAGLNGDLGF
                     SAADRTENIRRLAEVATHLARNGHIAIVAAVSPSAEDRAAARRIADTAFREVYVATPA
                     DICESRDPKGHYAKARAGALQAFTGIGKDYQPPTDCELRIDTSTRSVTDATDEIERML
                     AESGVLFDELVDLAANI"
     gene            complement(7549..8343)
                     /gene="cysD"
                     /locus_tag="VN968_11040"
     CDS             complement(7549..8343)
                     /gene="cysD"
                     /locus_tag="VN968_11040"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HXL29811.1"
                     /translation="MDHLDALEAQSIYIFREAFARLKKLALLWSLGKDSNVMIWLARK
                     AFFGRVPFPALHVDTGKKFPEMYAFRDRYGKEWELDLKIEPCPSLETIDPTLPPAARS
                     AARKTEGLKLALAKHGFDGLIAGIRRDEEATRAKERVFSPRGTEGGWDVRDQPPEFWD
                     QFNASPPPGAHLRIHPILHWTEADIWAYTQRENIPIIPLYLAKEGKRYRSLGDADITN
                     PVASTASNIEEILIELDGTKVPERAGRALDHETEDAFERLRVAGYL"
     gene            8610..10046
                     /gene="cysG"
                     /locus_tag="VN968_11045"
     CDS             8610..10046
                     /gene="cysG"
                     /locus_tag="VN968_11045"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HXL29812.1"
                     /translation="MRFLPVFLDLQTGPVLLVGAGDLARAKLRLLGAAGARIRWFATD
                     GRHDVSGLAAADAARIEFAEGDPLTADLSGVIAILCAGAGEIGPAMSARAKAVGLPVN
                     VMDDLAHSTFIFPAIVDRGDVVVAVGTGGASPVVARRVRERIEAMLPARIGDLATFID
                     RWRKPLHTRISEFALRRRFWERVVDGPIGALVLAGRSSEAETALKEISDPAAFAGALV
                     SGRAEGRVTLVGAGPGDPDLLTIKALRALQDADIVFYDELVSPEILDRARRDASRVPV
                     GRRVGKPGIGQHAINRLLIEAARSGQRVVRLKGGDPFVFGRGGEEVEALRDAGVAYSV
                     IPGITAGLGAAAQFEVPLTFRHEALRITFLTAHKAKDAEVVDWSTLTDEKMTVVVYMG
                     MTAAASVRAGLLAAGRSPHTPVGVFARVTRPDAEAAVGTLDELPDLIEKIDGGPAVLI
                     IGDVVAHSAPWRHPHLNHVISELLEAAE"
     gene            10043..10360
                     /locus_tag="VN968_11050"
     CDS             10043..10360
                     /locus_tag="VN968_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HXL29813.1"
                     /translation="MTSPLEQKKIRITGPSMVTANRTWDGVVIYRTASQSWSADLSDA
                     AIVRTSDEARALLTESIADDIGAIGAYIAPVEIKDSGKIKPGNLREHIRSEGVTIDLP
                     VPA"
     gene            10373..>11703
                     /locus_tag="VN968_11055"
     CDS             10373..>11703
                     /locus_tag="VN968_11055"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HXL29814.1"
                     /translation="MYAYDELDRTIINERVSEFRDQVKRRLSGELTEDEFKMLRLQNG
                     VYLQLHAYMFRVAIPYGTLSSKQLRALAHVARRYDRGYGHFTTRQNIQFNWIKLAELP
                     DALADLAEVGIHAMQTSGNNTRNVTSDQWAGVAPGEVEDPRIWSELLRQYTTLHPEFS
                     FLPRKFKFAITASDHDRAAIKVHDIGLRLYKNEAGETGFEVLVGGGLGRTPFIGKTIK
                     PFVHGHDLLSYVEAILRVYNQYGRRDNIYKARIKILVHELGIEKFAKEVEEEWLQMRD
                     SALAIDDSVIEDIRSRFSYPAYEKLPHMPEELKKAAHDPHFEAWRKNSVAPHKVTGYS
                     IVTLSLKPVGGPPGDATADQMDAIADLADKYSFGEIRVGHEQNLALPHVARRDLPVLW
                     KALDRIGVATPNVNLVSDIIACPGLDYCSLANARSIPIAQELTRRFANHETA"
BASE COUNT         1995 a         3615 c         3759 g         2334 t
ORIGIN      
        1 gctggtcgcg ggatgcacct gcaacggcaa ggatcaggtc ggtctggccc cggtcagaat
       61 cgaaaacgat ccgacgcttc gcaaggggga catcgtcgcc ggcagcgacg ggctggtggt
      121 cgccggccgc ggcgccgaca agcgcggcgc gtcgttgaat ttctcgccgg cgtcgttggc
      181 ggtgcgcgcg cgctatcagc gcgtgccggt tctcgcggcg gaatagccgg ccgcgccggc
      241 ggtgtgtccc aactctccca tcgaagtcgg atatatccga cttcgataaa taaagatgct
      301 caactcgggt aaacccgagt tgagggggag agggagcacg cttccagcgc cgaggggtgg
      361 atcggactct acgccgcgcg aatttttacc aggaactggg tcacctgatt gccgagatgc
      421 tgactctggg tttccagctt gccggaggcg cgtttcacat cgtcggccgc ggccgcggca
      481 gcgtccgcgt cggctttgac gccggtgatg ttgtcggaga cgttcctggt gccttgcgat
      541 gcgatctggg tgctgcgggc aatttcctgc gtggccgtgc cctgttgctg caccgccgcc
      601 gcgatggcgg ttgcgacctc gttcacctcg ccgatgatgc tgccgatgcc cttgatggcg
      661 tcgatggctt cgccggccac cttctggatg tcggcaatct gttccgaaat atcctcggtc
      721 gccttggcgg tctgactggc cagcgatttg acctcggacg ccaccacggc aaagccgcgg
      781 ccggcttcgc cggcgcgcgc cgcctcgatg gttgcgttga gcgccaaaag gttggtctgg
      841 gaggcgatgg tattgatcag gccgaccacc tcgccgatcc ggctcgccgt ctttgcgagc
      901 ccctggacgg ttccgtcggt ttgccgcgcc tgattgaccg cgcggctggc gatgccggcc
      961 gcatgcgcgg cttgctggct gatatcgttg atcgatgcgt tcaactcctc cgacgcggaa
     1021 gcgacgctct ccacgctcag cgaggcctct ccggacgcct tctccgcggt ctggacgcgc
     1081 gcgttggtct ggcgggagac cgtcgacagg cccgccgagg tgtttctcat ctgaccggaa
     1141 gcgtcgttca attggtcaag cgtttgccgt accgtgctct cgaattcgct gacgtaagcc
     1201 tcgatcgcct gctgacgcgt cgcggcgccg gcgttgcgtt cacgctcctg cgtttcaatc
     1261 cggatcttgt ccgcagcctg ttgcttgaat gtatcaagcg ccccggccag cgcgccgatc
     1321 tcgtctcggc gcgccgcata tccggtatcg acggtcagat cgcccgaggc cactttcagc
     1381 atggcgtcgc gcatgttgtg cagcggcttg atgacgcggc ggctgacggc catcatggcg
     1441 ccgaatgtca agccgagcgc cgaggccagc agcacgagct gcaacaccag cgagcgcagg
     1501 gctgccgaat gctggctgga ggtgtggtcc ttggcggctt cgagggcagc ctccgcgacc
     1561 ctgaccgcgc tactgaggcg tccgaccgtg atcggggtcc actggtttgc cgtcaattcg
     1621 ggcttcccgc cggccatcag tgtcgtcagc aggcgatcac gcaaggcgag ataatccggt
     1681 tcgaaatagg cggtcttggt cgcggccatc acggcggaaa gcgccggcgg caattgcgtt
     1741 cccgaggcgg tcaattccag cgcattccat gcgatctcgg tgccgcccac aaatttcacg
     1801 tatttcaggt gtgtttccgg cagcagctgt ccggcggtga tcgcgtttga aaccaggagc
     1861 gacgcctcgc ccgcggtatt gcggagcagc cacgcgatct gtttgatcgc gagcaattgg
     1921 tcgatcaccg gatcttgatg gttgaccgcg gccgcaagca tggcggacaa tttgtcgagc
     1981 gtcccgagca acgcggccgt cgtctccatg tattccttgg gcagggccgg acggcgggag
     2041 gccttcggct tgctcatttc gtcccagaat tccttttgct gcgcagtcag tgttttgaac
     2101 agacgatcca gctcaggtat cagcgtctgc tgctgcgcga attcgatgga cgggagcagc
     2161 gcgagcgcat tgcgcatggc gggcatttcg gtatcgcgaa gatcacgcag gtatttgtcg
     2221 atatcgctgt cgaccggctg atcggaattc aacagccggg tggtggtgga tcgatcggta
     2281 cgcagattat gcatggcctt gaacaggttc gccgaagcat cggcgatcac cgagatgcgg
     2341 ttcgtcaact gcaaccgccc ccaggactcc caggcgttga gcgaaaatcc gagcacaacg
     2401 cagaaagccg tggtcaggat gactgtcttg agcagcgcgg atacggtcag acgattcagc
     2461 ataattggcc cccaaatttc gcccatttgg acaaaaaagg gtaaacattt agacagccgc
     2521 tctgccggta gtattacggg atcttggcaa cacggatccc cctggccggt tgaacccgca
     2581 ggccagattg gatcgccccg ctgcggttcc tcaggcggaa cccggaggcg cgccgcgcgt
     2641 tgaggcggga ccaaagtcac ttctcacctt gtcagggggg ttcggatgct tgtcagtgcg
     2701 ctgatcacgt ttctggtcgt tattctcatt ctctatctga tcaacctgtt gccgatcgac
     2761 ggccgcggca agcagatcgc gcgtgtcgtc gtgatcatcc tcggcgtgat ttcgctgctg
     2821 aaatacatcg cggtgttcta ggccactggc ctacaaaaaa gccccggcga ataccggggt
     2881 tttttcgatc gctcagtggt gttaccttac ttcagcgtcc cgaaccagtc gtccacatcc
     2941 ctgtggatct gatccttggc aaaaccgtag cgctgctgca gcttgccttc gagctgttcg
     3001 cggcggcctt cgatcacgtt gagatcgtcg tcggtgagtt tgccccactt ctccttggcc
     3061 ttccccttga actgcttcca gttcccttcg acccggttcc aatccatcgt catctccctc
     3121 ttgcggttga gatgacgact taaccggcaa acaattcgaa cgttccgggc tagatgcgtc
     3181 gaatcaaccc accgccttgg cttcgcggcg ccgcgccgtc aggatgaact cggtataacc
     3241 gttcggctgc tcgcgcccct tgaagatcag gtcgcaggcg gccttgaagg cgacgccgtc
     3301 caaaccgggc gccatcggcc ggtacagcgg atcgcccttg ttctggccgt cgaccaccac
     3361 cgccatccgc ttcaaggact ccatcacctg ctccccggtg atgacgccct gatgcagcca
     3421 gttcgccaga tgctggctgg agatccgcaa ggtggcgcgg tcttccatca ggccgacgtc
     3481 gtagatgtcg ggcaccttgg agcagccgac accctggtcg atccagcgca cgacgtagcc
     3541 cagaatgccc tggcagttgt tgtcgatttc ctgcttcaca tggtcgggcg cccagttcga
     3601 ttgcgacacc gggatggtga gaatgtcgga cagttttgcg cgcgggccgc ctttggccag
     3661 ttcctgctgg cgcgcctgca cattgacctg gtgataatgc agcgcgtgca gggtggcggc
     3721 ggtcggtgag ggcacccagg cggtggtggc gccggcctgc gggtgcgcga tcttctgcgt
     3781 cagcatgtcc gccatcttgt ccggtgccgc ccacatgccc ttgccgatct gggcatggcc
     3841 gggcaggccg tcgatcaggc cggtatcgac gttccaatct tcataggcct tgatccaggg
     3901 ctgcgccttc atgtcgttct tgcggatcat cggtcccgct tccatcgagg tatggatctc
     3961 gtcgccggtg cggtcgagga agccggtgtt gatgaacacg atgcgcttcg aggcgttctg
     4021 gatgcaggcc ttgaggttga cggtggtgcg gcgttcctcg tccatgatgc cgaccttcat
     4081 ggtgttttcc ggcagcgaca acaacttctc gacctcgccg aacagctcac aggtcagcgc
     4141 cacctcgtcg gggccatgca tcttcggttt cacgatgtag accgagccgg tgcggctgtt
     4201 gcgcgtcttc gaaccgccct tgagatcgtg aatcgcgagc aggcccgcga cagcggcatc
     4261 cagaatgcct tcggggattt cggcgccggc cgcatcgagc accgcgtcgg tgaacatgtg
     4321 atggccgaca ttgcgcatca atagaagact acgcccatgc agcttgagct ccttgccgtc
     4381 ggaggtcttg tagacacggt ccgggttgag cgcgcgcgtc agcgtcttgc cgcccttctc
     4441 gaaatcggcc gacaacgtgc cgttcatcag gccgagcgtg ttgcgataga tcagcacctt
     4501 gtcttcggca tcgaccgccg cgacgctgtc ttccatgtcg aggatggtgc tgacggccga
     4561 ttccaggatc atgtcggcga cgccggccgg atcgtccttg ccgatcatgt ggccgcggtc
     4621 gattttgatt tcgacgtgca tgccgttgtt gaccagcagg atcgcggtcg gcgccgccgc
     4681 gtcgccctga tagcccgcga actgcgtgtt cgatttcagc gcggtggcgt tgccgctctt
     4741 tagcttgacc gagagttgcc cggcgatgac actgtaggag gtcacatcgg tgtggcttcc
     4801 ggttgccagc ggcacggcgg catcgagaaa ggcctttgcc ttggcgatga ccttgtcgcc
     4861 gcgcgtcttg ttgtagccct tgccgctttc accggcgtcg tgcgggatcg catcggtgcc
     4921 gtaaagcgca tcgtaaaggc tgccccagcg ggcgttggcg gcattcagcg cgtagcgcgc
     4981 gttggtcagc gggaccacca gctgcggtcc gcaaatcttt ccgatctctt cgtcgacatt
     5041 cgaggtttcg accttttgcg tcgccggttc cggcaacaga tagccgatct ccttgaggaa
     5101 cgcggtgtac ccattcatgt cgaacggctt gcccttgttg gcgcggtgcc agccgtcgat
     5161 tttcgcctgc aaactgtcgc gcaccgcgag cagctcttgg ttcttcggcg cgagattttg
     5221 gatgatggcg gcgagccctg cccagaatgc atcgggcgaa gtccctgttt tgggggtggc
     5281 ctccttggcg atgaaatcga acaaaacggg ggcgatcttc aatccgtggg catcgacacg
     5341 attcatgacg ggttttctcg cgagaaacgg cggttacgcg ccgtctacag ggacaactga
     5401 caaaagcgcg ccgcaaacgg cggcgttcac ggccctttta gcgctaaagc cggggccaag
     5461 agaaggccct tggcggcgtt agattgccca taacagaggc tggagcatgg tccgcaaaaa
     5521 agtggaaacc ggttgtccct cgggacaaac gcgaagcgtt tgcccggagg tcatgctcaa
     5581 gcaaaagata aagcgaggtc agatattggc ggcgaggtcg accagttcgt cgaacagaac
     5641 gccgctctcg gccagcatcc gctcgatctc gtcagtggcg tcggtgaccg agcgcgtcga
     5701 ggtatcgatc cgtaattcgc agtccgtcgg cggctgataa tccttgccga tcccggtaaa
     5761 cgcctgcagc gccccggcgc gtgccttggc gtaatggccc ttgggatcgc ggctctcgca
     5821 gatgtcggcc ggtgtggcga catagacttc gcgaaatgcc gtgtcggcga tgcggcgcgc
     5881 cgcggcacga tcttccgccg acggcgagac cgcggcgacg atggcgatgt gcccgttgcg
     5941 cgcgagatgg gtcgcgactt cggcgagccg tcgaatgttc tcggtacggt cggccgcgga
     6001 gaagccgaga tcgccgttga ggccggcgcg caaggtatcg ccgtccagca gcaccggcga
     6061 accgccctgg ctgaacagtc ggcgctccag cgcgcgcgcc agcgtcgatt tccccgaacc
     6121 cggcagtccc gtcagccaga ccaccgctcc gccgtgacga taacgcgccg agcgctcgtc
     6181 cgggcgcagc gcggattcca ccggcacgat atcgacgggg acagcgcgct gtccggcatc
     6241 gaccgacagc accagaccgc cgccggcaat gcggccgttg acctcgatca ccagccgtcc
     6301 ggtgcgcgga ttatcggtat agggatcggc ggcgatcgcc tgcgccagcg agatgtcgat
     6361 ctcgccaacg tgattgcgcg cgatcgattt tgtttcgatg ctcgagagtt cgccgggatc
     6421 gacggccttc tcgatcgcga ccacggtggc gcgggtttcg cgggtgccga gccggaccag
     6481 gatcgaagcg cccgatacca gcggcttgtc atgcagccag aaaatccgcg cgcggatgcg
     6541 gcgggtatcg cgcgggctat cgccgacatg ggcaatcacg tcgccgcgtt cgatgaacag
     6601 ttcgcggtcg agcgtgatgc cgaccgatcg cccggcgctt tggctgcccg tgaccggcgt
     6661 aaccggccag ccctcgacgg tcttgatctt cgcgatcttg cccgcgggca tgatgacgat
     6721 ctcgtcgccc gcgctgaggc ggccggattc gatgcgtccg gcgacaatgc ggcggtcgtc
     6781 gaatttgtag atcgcctgca ccggcagccg cagcgccagc gtttccagcg gccgcgccgg
     6841 ttcaagcgcg tcgagcgcct cgatcacggt cggtccattg taccattggg tccgcggcgt
     6901 gcgctcggcg acgccgtcgc cgtcgcgcgc tgaaatcgga atcaccgccg aaggctgcaa
     6961 cccaaggccg atcagatgcg cggaaatctc gtcgcggatc tcggcaaagc gcgccgcgcc
     7021 gaaatcgacc cggtccatct tgttgacgac caccgcgacc tgtttgacgc cgagcagatg
     7081 caacagatag ccatggcgcc gtgtctggtc gcgcacgccc tccagcgcgt cgatgatcag
     7141 cagcgcgcca tccgcctgcg aggcgccggt aatcatgttg cgcaggaatt cggcgtggcc
     7201 gggcgcgtcg atcagcacca cgtcgcgcga gcgggtgcgg aagctgatct gcgtggtgtc
     7261 gatggtgatg ccctggtcgc gttcggtctg cagcgcgtcc aacagaaacg accattcgaa
     7321 cggcatgccg cgccgcgcgc tgacggcttt cagcatttcc agcttgcctt cgggcaggct
     7381 gccggtttcg tgcaacaggc gcccaaccag ggtcgacttg ccgtgatcga catggccgac
     7441 aatgacgatg cggacctgcg ggcgtgtggt gccgttcggc gtcgccgata tcagggatgt
     7501 cgcggatggg accatcatgt tcatcggatc actcaactca ctatcggttc agagatagcc
     7561 ggcgacgcgc agccgctcga aggcgtcttc ggtttcgtgg tcgagcgcgc ggcccgcgcg
     7621 ttccggcacc ttggtgccgt cgagctcgat caggatttct tcgatgttgg aggcggtcga
     7681 tgccacggga ttggtgatgt cggcatcgcc cagcgagcga tagcgcttgc cttccttggc
     7741 gagatacagc gggatgatcg ggatgttctc gcgctgggtg taggcccaga tgtcggcttc
     7801 ggtccaatgc aggatcgggt ggatgcgcag atgggcgccc ggcggcggcg aggcgttgaa
     7861 ctgatcccag aattccggcg gctgatcgcg cacgtcccat ccgccttcgg tgccgcgcgg
     7921 cgagaatacc cgctccttcg cgcgcgtcgc ctcttcgtcg cggcgaatgc cggcgatcag
     7981 gccgtcgaaa ccatgtttgg cgagcgcgag tttcagtccc tcggtcttac gcgcggcgga
     8041 tcgcgccgcc ggcggcaagg tcggatcgat ggtttccagc gacgggcagg gttcgatctt
     8101 cagatcaagc tcccattcct tgccgtagcg gtcgcgaaac gcgtacatct cgggaaattt
     8161 cttgccggtg tcgacgtgca gagcggggaa cggcacgcgg ccgaaaaacg ccttgcgcgc
     8221 cagccagatc atcacgttgg aatccttgcc gagtgaccac agcagcgcca gcttcttcag
     8281 ccgcgcgaag gcttcacgga aaatgtagat gctctgggcc tcgagcgcgt ccaaatgatc
     8341 catcgtgtga tccttggtcg gtgcacggga gtgcccattg atcagtattt gcggggccgg
     8401 agatcccttg gctgggctcg cgcggtgtaa acggtctttg gcggaatcgt tgtcgagaag
     8461 atgcatctct gccccttggc ctttggaggc taaaattcta tagttgcact gcagtagaga
     8521 agaaataatt ttctctttgc ggcgcttgaa cgagacatat atagaaaata attccagtca
     8581 accccgcaag tgggggaagc gagtatcgca tgcgatttct gccggtattt ctcgatctgc
     8641 aaaccggtcc ggtgctgctg gtcggcgctg gcgacttggc gcgggcgaaa ttgcggctgc
     8701 tgggagcggc gggggcccgc atccgctggt tcgcgaccga tggccgtcat gatgtgagcg
     8761 gattagcggc tgcggacgcg gcgcggatcg agtttgccga gggcgatccc ctgacggccg
     8821 atctctctgg cgtgattgca atcctgtgtg caggcgcggg cgagattggg cctgcgatgt
     8881 cggcgcgggc aaaggcggtc ggcctgcccg tgaatgtgat ggacgacctt gcgcattcga
     8941 ctttcatttt tcccgccatc gtcgaccgcg gtgacgtcgt cgttgccgtc ggcaccggcg
     9001 gcgcgtcgcc ggtggtggcg cggcgcgtgc gcgagcggat cgaggccatg ctgccggcgc
     9061 ggatcggcga tctcgcgacc tttatcgacc gctggcgtaa accgcttcac acccgcattt
     9121 ccgaatttgc gctgcgccgc cgcttctggg agcgcgtggt cgatggcccg atcggcgcgc
     9181 tggttctcgc cggacgcagc agcgaggccg aaacggcgct gaaggaaatt tcggatcctg
     9241 cagctttcgc cggcgctttg gtgtcaggcc gggcagaggg cagggtgacg ttggtcggcg
     9301 ccggtccggg cgatccggac cttttgacca tcaaggcgct gcgggcgctg caggacgccg
     9361 atatcgtgtt ttacgacgaa ctggtctcgc cggaaattct cgatcgcgcc cgccgtgacg
     9421 cctcgcgcgt gccggtgggc cgtcgcgtcg gaaaacccgg catcggccag cacgcgatca
     9481 accgactgct gattgaagcg gcaaggtcgg ggcagcgtgt cgtgcgcctc aagggtggcg
     9541 atccctttgt gttcggccgc ggcggcgagg aagtcgaagc gttgcgcgac gccggcgttg
     9601 cctattcggt gatcccggga attaccgcag gccttggcgc cgccgcccaa ttcgaagtgc
     9661 cgctcacctt ccgccacgag gcgctgcgca tcaccttcct gaccgcgcac aaggccaagg
     9721 atgcggaagt cgtcgattgg tccacgctga ccgacgagaa gatgacggtg gtcgtctata
     9781 tgggaatgac cgcggcggcc tcggtccgcg ccggtctgct cgccgccgga cgctcgccgc
     9841 acaccccggt cggcgtgttc gcgcgggtga cgaggccgga tgcggaagcc gccgttggca
     9901 cgctggatga attgcccgat ctgatcgaaa agatcgacgg cggccccgcc gttctcatca
     9961 tcggcgacgt ggtcgcgcac tccgcgccat ggcgccaccc gcatctcaac catgtgatct
    10021 cagaactcct ggaagctgcc gaatgacctc tccccttgaa cagaaaaaaa tcagaattac
    10081 cgggccgtcg atggtgaccg ccaaccgtac ctgggatggt gtggtaatct atcgcaccgc
    10141 ctcgcaaagc tggtcggcgg acttgtcgga cgccgcgatc gtacgcacct ccgatgaagc
    10201 gcgggcgctg cttacggaat cgatcgccga cgacatcggc gcgatcggcg cctatatcgc
    10261 tcctgttgaa atcaaggact ctggcaagat caagccgggc aatctgcgtg aacacatccg
    10321 gtcggaaggc gtcaccatcg atcttccggt tccggcataa ggccagccta ttatgtacgc
    10381 atatgacgag ctcgaccgca cgatcatcaa cgaacgtgtt tcggaatttc gcgaccaggt
    10441 gaagcgccgg ctgtccggcg aactcaccga ggacgaattc aagatgctgc ggctgcagaa
    10501 cggcgtttat ctgcaattgc acgcctatat gtttcgtgtg gcgattccct acggaacgct
    10561 gtcgtcgaag cagttgcggg cgctggcgca cgtcgcacgg cgctatgatc gcggctatgg
    10621 ccacttcacc acccggcaga acatccagtt caactggatc aagctcgcgg agttgccgga
    10681 tgcgctggcc gatctcgccg aggtcggcat tcacgccatg cagacctcgg gcaacaacac
    10741 gcgcaacgtc acatcggacc agtgggccgg cgtggcgccc ggcgaggtcg aagatccccg
    10801 catctggtcc gaactgctgc ggcagtacac cacgctgcat ccggagttct ccttcctgcc
    10861 gcgcaagttc aaatttgcga ttaccgcttc cgatcacgac cgcgcggcga tcaaggtgca
    10921 cgatattggt ctcaggcttt acaagaacga agcgggcgaa accggatttg aggtgctggt
    10981 cggcggcggc ctcggtcgga cgcccttcat cggcaagacc ataaaaccgt tcgtgcacgg
    11041 ccacgatctc ttgagctatg tcgaggcgat cctgcgcgtc tacaaccagt acggccgccg
    11101 cgacaacatc tacaaggcgc gcatcaagat cctggtccat gagctcggca tcgagaaatt
    11161 cgcaaaagaa gtcgaggaag aatggctgca gatgcgtgac agcgcgctgg cgatcgatga
    11221 ttccgtcatc gaggacatcc gttcgcgctt cagctatccg gcctacgaaa agctgccgca
    11281 catgccggag gagctgaaga aggcggcgca tgatccgcat ttcgaggcgt ggcggaagaa
    11341 ttcggtcgcg ccccacaagg tgacgggcta ttcgatcgtg acgctgtcgt taaagccggt
    11401 cggcggaccg cccggcgatg ccaccgccga ccagatggac gcgatcgccg atctcgccga
    11461 caaatattcc tttggcgaga ttcgcgtcgg ccacgaacag aatctcgcgc tgccccatgt
    11521 cgcgcgacgc gacctgccgg tgctgtggaa agcgctggac aggatcggcg tcgcgacccc
    11581 gaacgtcaat ctggtgtccg acatcatcgc ctgcccggga ctggattatt gctcgctcgc
    11641 gaatgcgcgt tcgatcccga tcgcgcagga attgacccgg cgcttcgcca atcacgagac
    11701 cgc
//
LOCUS       DATNOI010000286        23611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_897376,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000286 DATNOI010000000
VERSION     DATNOI010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 23611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23611
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897376"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VN968_11060"
     CDS             complement(<1..591)
                     /locus_tag="VN968_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXL29815.1"
                     /translation="MGRFFRIKLRLRRFARRHRWLISSMRSLVVFSAAFGGAYGFIVG
                     SRAENSGYDANAFAIGASFLFAVACVALATVSMRLRFLRKKLRMIALHNEALADRNWE
                     LQEAEQHARSLFESQSDLIVMRDAEGRITFVNDAYCQMAEQPREALIGGRFVFEVLEQ
                     GDDALDPNGTRIHDQQIATRLGPKWIAWREGLVRSDA"
     gene            complement(745..1983)
                     /locus_tag="VN968_11065"
     CDS             complement(745..1983)
                     /locus_tag="VN968_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HXL29816.1"
                     /translation="MILAGQARAARKRYREMTPVLNEQSRRRFVALEARALGRGGVSL
                     MARISGLARSTIYHGLSDIRDQTSVPSGRVRKPGGGRKKKSIQDPTLVVDLKRLVEPV
                     TRGDPMQPLLWMTRSLRNLANELANKGHQVCPTVVGDLLRGMGYSLQANSKTREGGQH
                     IDRDAQFHYINTQAKAFLAANEPVISVDTKKKELVGNFKNNGREWRRNGAPELVNVHD
                     FIDPKLSRAVPYGVYDITNNVGWVSVGTDHDTATFAVNAIRRWWRTMGRKRHPKAKRL
                     MITADGGGSNGYRVRLWKVELQKLAEELRLPITVCHLPPGTSKWNKIEHRLFSFITIN
                     WRGKPLRSYRTIVQLISATTTDTGLKVRAELDENKYPKGVKVSDFQMAAVNLTRHSFH
                     GDWNYTVSPKRNNPRQKRID"
     gene            complement(2174..3022)
                     /locus_tag="VN968_11070"
     CDS             complement(2174..3022)
                     /locus_tag="VN968_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29817.1"
                     /translation="MSASLTLRQRFLAWLADNFDEALLRWIFRCLVAVTIAVLGADLA
                     GKTGWIGKEDSTSAPAEIRQDSLSPDSPGVLPSILAPLLPGGDKRLMPLQQPDGALAQ
                     AMTFELAGGGRLMATGTITPGSSQAFAAEIERRGDYVKTVVLNSPGGSVMDAQAMGRL
                     IRERKFATEVEAGKYCASSCPLMFAGGLERRASDRAIIGVHRIAAIQSAANTPPRDEM
                     SLAQNISARCERYLREMGVDLQVWLHAMETPHDKLFVFKPDELKSLALVTSPTGPQNP
                     ANKNAR"
     gene            complement(3028..4413)
                     /locus_tag="VN968_11075"
     CDS             complement(3028..4413)
                     /locus_tag="VN968_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29818.1"
                     /translation="MLSNSPSTSQSSSARVSTSVLLANGVSRLGATLKLTTAILALAA
                     GVYTYLGVRELLNGSPTTVFFAAVIYSVAVSVGIYTFWVFLMQFMPHVVSSTGRGLMF
                     GCMLLGSLMIVAMSSWLNASALAGAAAIQQHLAITVQDYTRDLDTANTNAIAAQGLLP
                     DIQLASSRFARLADAERAGSLTGTAGSGTVVQLLTQMSGQLDSLGQQVQDSAKRVSAL
                     YEQGGKNLAKMRELISDRGPIAARSDAFATESLALMGVIANLQQTSVAPAVKRAATAL
                     SSGFIAPAAGGRTSDLADRQTAVVGKVESSIATQAASLADAADKILAMRHVEPARFQT
                     LSPAEAVLRYAGDFIPSWAGAISIDLMPAVLVLILCVVHAAIRREGLPVVNASNITAS
                     ELVSALQIAREVEEARRAAEEAKPAPKPEPEPKPEPAVAEEAKPTAPEETEPAVPEEN
                     VTSLSSARYTK"
     gene            4573..4856
                     /locus_tag="VN968_11080"
                     /pseudo
     CDS             4573..4856
                     /locus_tag="VN968_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012758720.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(4857..5927)
                     /locus_tag="VN968_11085"
     CDS             complement(4857..5927)
                     /locus_tag="VN968_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HXL29819.1"
                     /translation="MSEAVIDDIVAVLPPLLQSIEALAFVARHLNPPDFDRVMQTIGA
                     PEEALQTVRTRLTERPEQFADVRASLERSSDAALAAFAGMRAVQHGNGDLVAVFRALR
                     YLPRAQEALYPLAEKFPPVSDFFLDPALRDDDALKARLAAPRSEHTGVTHDRDDPGSR
                     GGFSLYVPEYYTPDSAWPLVMALHGGSGNGRNFLWSWLRDARSFGAILVAPTATGPTW
                     ALMGDNTDTPNLLRILDDVGSRWNIDSKRLLLTGMSDGGTFCYVTGLDGASPFTHLAP
                     VAATFHPLMAEMADAERLRGLPVFLVHGRLDWMFPVQVARHTQQALSAAGANVTYREI
                     ADLSHCYPREINAEMLTWLNGP"
     gene            complement(6050..7021)
                     /locus_tag="VN968_11090"
     CDS             complement(6050..7021)
                     /locus_tag="VN968_11090"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HXL29820.1"
                     /translation="MPSVTEWKVPPAFQPRPSDYRFDLDRALSSVVGLHSLIPADAFT
                     AETLGTERAGNGVVIEDGLVLTISYLITEAETVWLHLGDGRVMEGHALGFDPVTGFGL
                     VQALGRMDRNPLPLGSSAAARLGDRVVVGGAGGRTRSVASHIAAKQEFAGYWEYLLDE
                     AIFTYPAHPNWGGTALIANSGELIGIGSLQLERERAGKAEHVNMIVPIDLLKPVLDDL
                     RKFGRVNRPARPWLGMYSTEIDNRVVVIGISGNGPAARAELKAGDVILAVKGERITSQ
                     SAFYRKLWSLGPAGVDVPLTVHHEGVTFDVVLASTDRTKLLKGPRLH"
     gene            complement(7193..10531)
                     /locus_tag="VN968_11095"
     CDS             complement(7193..10531)
                     /locus_tag="VN968_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL29821.1"
                     /translation="MDVGTWLGSLGLDQYEDLFREHEIEADILPELTDEHLRDIGVPL
                     GHRLRMLRAIRHLARKAQPDSNPRPQENPERRQLTVMFCDLVGSTALTAQRDPEDMAD
                     LIRAFQGAVAAPVARFDGHVAKWTGDAAMVYFGYPRAHEDDAERATRAGVALIDAVAE
                     LRRHHDTSLEVRVGIATGLVVVGELIGEGEARLRGLVGDTPDLAMRLQGFAEPNTIVV
                     SESTRRLLGKTFELKALGPQTLRGFDVPVAAWLIAGERENVSRFEASRSETLTPFVGR
                     EQEIALLDERWQRAMKGEGQVALLSGEAGIGKSRVLAMLRQQIGKQNYLVLRYQCSPH
                     HINDAFHPVMGQIWYAADFVSGEAAGSRLDKLEKMIESTGLSNRDIAPYLASLLAIPT
                     GQRYPALDLAPSELRERTIAALIAMTVGAAKQIPLLMIVEDAHWIDPTSLDLMNRLVE
                     QMRHLPILLVTTYRPEFTAPWLGRDNVTTVPLNRLERDQAVRMIERMTSGKKLPADVL
                     DQIVAKTDGVPLFMEELTKSVLESGLLREENGTYVLASALTPLAIPSTLHDSLAARLD
                     RLSPIKETAQIGAAIGREFSQALLEAVSPIKGAALDEALRQLMEAELIHIRGAPPKAS
                     YVFKHALVQDTAYGSLLRSRRERIHAHIAWALKQRLTDEEYPPAIIAHHYTEAGLSDQ
                     AARSWLAAAELALSQSAPVEAERHASIGLALIPSIEAGTERDALELGLLVARANALVP
                     LKSISAPETFAALMAAKQLLDRGIGTDLQRVSILYGLCSSSTLTARLKQALDFAHQII
                     EVADRQNDPAYQLVGYRQLGTLQFYAGNFREALANLQKGHTYRDARRQKALSYRFSWD
                     QGIAILSFEVLVRLSLGQLDSAARVSEQVRSELSSHGHTTTIASATFCVTVWPQFALR
                     DIEALERDSAELSAYCAEKKVEQIRLLAGLLGACASAMRAPTESRIVSIREALVALRQ
                     SGGNTGNSILLSNIAEAFLIAGDLSGAEAALQDGFAFVEQSGEQYWLADLHRLSGQVA
                     LRQVEPDRARAEACFTKAIEVARNQQARLLELRAATDLARLRRGSRANSDIRGLIEPI
                     LAMIEGGETARDVREARALLAELH"
     gene            complement(10736..12253)
                     /locus_tag="VN968_11100"
     CDS             complement(10736..12253)
                     /locus_tag="VN968_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011082963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXL29822.1"
                     /translation="MLIPMADVPRWYAERKPDDTIAISHGADTLTWGELERGANARAR
                     AFAEKGVKPGDFVAIGLPNGNAFFETSFAVWKLGATPTSLSWRLPRGEAAAVLDVLKP
                     ALVVGGEADWNAPTALPADFVPEGFSAEPLASPVARYWKAMTSGGSTGRPKVILDHAP
                     AVVDTASEPPLGIPQGASLLNPGPLYHNAPFILSHSCLFAGGRLTGLAKFDAEEALRL
                     IETHRVQWVNFVPTMMHRIWALPDEVRNRYDLSSLRIVFHMAAPMPPWLKEKWIAWLG
                     PERIWELYGGTERQGACVISGVEWLAHKGSVGKIGETARLRIIGEDGNDVAPGETGEI
                     YFLPNDGPGSTYHYLGAEPKRRADGWESLGDIGRLDADGYLYLGDRLADMILRGGANI
                     YPAEVEAAVSAHPDVRSCVAVGLPDPEFGQRVHAILELDETADAQAIADGMGDFLSDR
                     LSRYKHPESFEIVRIGLRDDTGKVRRTLLRDERAAWLKENRAFRIMPSRARAKAQ"
     gene            complement(12455..13222)
                     /locus_tag="VN968_11105"
     CDS             complement(12455..13222)
                     /locus_tag="VN968_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXL29823.1"
                     /translation="MKAILPLIVAGVIGLIDPAQARSLETIRSYGTIGLCAHPNSLPF
                     ASKAGDPPGFQVELAQALARELGVSLRLDWIITQYQLRSAGCDIVMDVIADREAQGET
                     NLAISKPYYRTGVALAVPSASKLTSFKSLDEHTKVGVQVGSIAAMIISQRHVPTSTFG
                     FEVDSLEALANREIDAAAVTPTIASYFNQTRRDNAVRILDRDESETNLNWNVAVGMVR
                     PDQKLREAIDAALERLHGDGTIDRIYGRYGVVLQSPR"
     gene            complement(13263..13610)
                     /locus_tag="VN968_11110"
     CDS             complement(13263..13610)
                     /locus_tag="VN968_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXL29824.1"
                     /translation="MKLDMDVKALLPAVLAVSLICALPARAQPAANAPDSGAFDVEQL
                     FASTCGFCHSSGGRAAGRGPQLMDSPRSDDFIRDRIKNGKNGAMPAFGATFTDAQIED
                     IIKYIRSLKPREG"
     gene            complement(13698..15422)
                     /locus_tag="VN968_11115"
     CDS             complement(13698..15422)
                     /locus_tag="VN968_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXL29825.1"
                     /translation="MTKQSNYLALPVAVVIAATFAASSTFAQSVDTARIEAGGQNDWL
                     TYHGSYKSHHYSPLAQINPSNVANLGVAWIHIPGRSTRGLQSMPLVADGVLYYTGSYS
                     RTFALNGATGEVIWSYFPELDEALIARQTHSPYNRGVALGEGKVYVGTMDGRLIALDM
                     KSGKPLWDTKLIDSQKLTVGFTGAPLYAKGTVVIGSQGGEWPGRGPIFGVDAATGKKK
                     WEFLTVAGTDEAMKTWGSDSWRTGGGGGWMPGTYDSDTNTILWGTANPAPLYDWSGND
                     YKTQGARPGDNLYTSSVIALDVDTGKLKSYHQELPHDAWDFDSAVGEFLMLDRDGQKL
                     IVHPNKSGFIFVYDRNLAVKNVWRLVQNINFVKDIDPKTGELIGRRDFTAGKVEAPPL
                     CPYIGGGVSWNSGSYNPKTGLYYKIGQEWCMTLDVVKTTPVTAPQAQLNIGADFKIAP
                     PPGGEIYGHLDARDPVTGAKKWEVRFPEPPIASVLSTAGNLVFVPDSRGVVHAYDAET
                     GTELWNHSNGTGHQGGIVSYSVGGKQYIAMTAGFGGMLSDDYAATFGGVYKSMPRDDG
                     ALIVYSLK"
     gene            16416..16700
                     /locus_tag="VN968_11120"
     CDS             16416..16700
                     /locus_tag="VN968_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29826.1"
                     /translation="MNEPDNEMFAAAAIERALGIYQQLRERDRSVILQARKILTQHIY
                     GMFDKGEHDEKRLVVSGLARLTSIERDHAIKSAHDVPAASTGKRRRRASA"
     gene            complement(17008..17310)
                     /locus_tag="VN968_11125"
     CDS             complement(17008..17310)
                     /locus_tag="VN968_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29827.1"
                     /translation="MRKLTLTFLAGAGALLATSAYAADRIQTGDNSALLHQARLVCND
                     AGQCWNTRGDRRVIIGGRESYYGPRYYDDYDRGYYHRGPGVGVRGPGFSFGIGPDY"
     gene            17599..18033
                     /locus_tag="VN968_11130"
     CDS             17599..18033
                     /locus_tag="VN968_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017964713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXL29828.1"
                     /translation="MRVAETMTRGVFIVSPEETIQEAAMAMAGIDAGVLPISNGDRLV
                     GMITDRDIAIRGVAEGKGPDTKVRDVMTTEIKYCFDDQEVDDVIRNMGEIQVRRLPVV
                     DRDKRLVGILSLGDVAATASDGKAGEALSKISRPGGEHTQTG"
     gene            18474..19970
                     /locus_tag="VN968_11135"
     CDS             18474..19970
                     /locus_tag="VN968_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXL29829.1"
                     /translation="MTEGRTRGRMRRALAGSILAIGIVGFGTVAWLVGSRVSTWRDLY
                     IWYNPAVGFQSAALPVGFEDLVERIKPTVVGVRAKVERDADEEDQPAGHGAPSSRSDL
                     PKGNVGANQPHVATSQGSGFFISSDGYAMTTNHVVERSEKIEVTTDDGKIYPAKLVGA
                     DPKTDLALLKIDGGNEFPAARIADKAPRIGEWVLAVGNPFGLGGTVTAGIVSARARDI
                     KIAAYNDFIQIDAPVNQGNSGGPTFDVGGNVIGVNSAIFSPTGGSIGIGFAIPAETVK
                     TIADRLKDKGTIRRGWIGIEVQSLSPEIVEGLGVNKSVHGVLVAETQPDSPAAKAGIV
                     PGDVITSLAGQPTSDDRDLVKRVGDMSPGKSIELRLIHQNEEKAISVTLGELPGPRNE
                     SGLTTEKQKSPAADGNQANIGLTLMPADKLGLVVGGVVVTELDTAGVAAESGLSIGDV
                     ILDVGGTVIKTPVDFYKALTEVQSRGGRVALARVKSNDATRFIAIPVG"
     gene            complement(20287..20844)
                     /locus_tag="VN968_11140"
     CDS             complement(20287..20844)
                     /locus_tag="VN968_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXL29830.1"
                     /translation="MNAHVSQGGWPVLVLNADFRPLSYYPLSLWSWQDAIKAVFLDRV
                     NIVEHYDRAVRSPSFEIQLPSVVSLKSFVKPSTHPAFTRFNVFLRDRFVCQYCSADDD
                     LTFDHIVPRSKGGQTTWENVVAACSPCNLRKGNLTPQQAKMFPRQAPFAPTVHQLHRN
                     GRLFPPNYLHDSWLDYLYWDTELDP"
     gene            21026..22003
                     /locus_tag="VN968_11145"
     CDS             21026..22003
                     /locus_tag="VN968_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL29831.1"
                     /translation="MRQWLALLIAAFIGLGSSNICKAQGYPARPITMIVPFPAGGATD
                     TVARFLGEKMRAILGQPVVIENVAGAAGSLGVGRAVRSAADGHTLSIGTSTTHVLTGG
                     LYALPFDLLRDLEPVIEIGSEPLLIVAKKSLPADDLKGLIAWLKANPDKASVGIAGVG
                     ATGHLTGISFQKETGTRFQFVPYRGNAPAMQDLLAEQIDFMIEPSSNFKALFGAGSVK
                     PFAITGRTRLRSSPDIPTADEAGLPGFHASLWYGLWVPKGTPKEIIAKLNATMSQILA
                     DPQVKQRFDELGIQITPLDQQSPEALRAFQKAEAERWWPIMKASNIKAE"
     gene            complement(22143..23117)
                     /locus_tag="VN968_11150"
     CDS             complement(22143..23117)
                     /locus_tag="VN968_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HXL29832.1"
                     /translation="MSVVLGTGEHRYRVVEDWAKLPDGWEFRDVGAVAVDSKDRVYVF
                     NRGAHPMVVLDREGNFITSWGEGLFARAHGLYIDADDNLYCTDDGDHTVRRCTTDGRV
                     LLTIGIPNKPAPFMSGEPFHRCTHTALSPKGEIYVSDGYGNACVHKYTPDGRLLKSWG
                     EPGTDPGQFNIVHNIATDADGWVYVADRENHRVQVFDGNGRYETQWNNLHRPCALHCC
                     GGKHPNFIVGELGPGMPVNLKVPNLGPRLTIVDAKGNRIARLGGEHGPGLQTGKFLAP
                     HGLALDSKGDIYVGEVGVTNWKSSFPDTPMPPEVRGVRCLQKLERIRE"
     gene            complement(23134..>23611)
                     /locus_tag="VN968_11155"
     CDS             complement(23134..>23611)
                     /locus_tag="VN968_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HXL29833.1"
                     /translation="FHRMAALQMSDALKAVTTDPFPASYSDRVRKIGVVNTKNKVVTA
                     IVAKLLDGPAALRSLLIDRFVVEGFNFHEVMTDDDALEAFIRKAAIGVWHASCSCRMG
                     RADDPMAVVDTAGLVKGVQGLRVVDASIFPVVPCANTNFPTLMTAEKIADAVLAGR"
BASE COUNT         4497 a         7116 c         7399 g         4599 t
ORIGIN      
        1 ggcgtccgac cgcaccaggc cctcgcgcca ggcgatccat ttcggaccga gccgggttgc
       61 aatctgctga tcgtgaatcc gggtgccgtt cggatcgagc gcgtcgtcac cctgctcaag
      121 aacttcaaag acgaagcggc cgccgatcag cgcctcgcgc ggctgctcgg ccatctggca
      181 ataggcgtcg ttgacgaagg tgatgcggcc ttccgcgtcg cgcatcacga tcaggtcgct
      241 ctgggattcg aacaggctcc gcgcatgctg ttcggcttcc tgcagttccc aattgcggtc
      301 agccagcgcc tcgttgtgca gggcaatcat gcgcagcttc ttgcgcagga agcgcagccg
      361 catgctcacg gtggcgagcg cgacgcaggc gacggcgaac aggaagctcg cgccgatggc
      421 aaacgcattg gcgtcatagc cggaattttc cgcccggctt cccacgatga atccataggc
      481 gccgccgaaa gccgcggaga agaccaccag cgaccgcatc gagctgatca gccatcgatg
      541 gcgtcgtgcg aagcggcgta accgcagttt gatccggaag aaccgtccca tcgccgttcc
      601 ttcaactcgt gccgttgacg tcttgtgatc gattcgttcg gcgacgatgc ggcggcgacg
      661 ttgcaaagtg cttgaaagca cgttgcttcg caccgcgcga tgcgaacagg gttaataaag
      721 cgttagggct cgtccataaa ttagtcagtc aatccgtttt tgacgaggat tatttcgctt
      781 tggtgaaacg gtatagttcc agtcgccgtg aaaagaatgg cgggtcaggt tgacagcggc
      841 catttggaaa tcggaaacct tgacgccttt gggatatttg ttttcgtcca gctcggcgcg
      901 caccttgagg ccggtatcgg ttgtcgtcgc agagatcagt tggacgatcg tgcgataact
      961 gcgcaacggc ttgcctcgcc agttgatcgt gatgaacgag aaaagacggt gctcgatctt
     1021 gttccacttg cttgtgccgg gcggcagatg gcaaaccgtg atggggagcc tgagctcttc
     1081 tgccaacttc tgaagctcga ctttccagag acgcacacga tatccgttgc tgccgccacc
     1141 gtcagccgtg atcatcaggc gtttggcctt gggatgccgt ttccttccca tcgtccgcca
     1201 ccatcggcgg atcgcgttga ctgcaaaggt ggcggtgtcg tgatcggtgc cgacgctgac
     1261 ccacccgaca ttgttggtga tgtcgtagac gccatacggt acagcacggc tcagcttggg
     1321 gtcgatgaaa tcgtggacgt tgacgagctc gggcgctcca ttgcgccgcc attcccggcc
     1381 gttgttcttg aaattgccta ccaattcctt cttttttgta tcaaccgaaa tgaccggttc
     1441 gttcgccgcc agaaatgctt tggcctgcgt attgatgtaa tgaaattgcg cgtcgcgatc
     1501 gatgtgctgg ccgccttctc gtgtcttgct gttcgcctgc aggctatagc ccatgccgcg
     1561 cagaaggtca ccgacgaccg tcgggcagac ttggtgtccc ttgttcgcca gctcgttcgc
     1621 gagattacgc aagctgcggg tcatccacaa cagcggctgc atcggatcgc cgcgcgtcac
     1681 gggctcgacc aaccgcttga ggtcgaccac aagagttgga tcctgaatcg acttcttctt
     1741 gcgtccaccg cccggcttgc ggacgcgtcc gctcggaacc gacgtctggt cacgaatgtc
     1801 ggaaagtccg tgataaatcg tcgaccgtgc cagtccgctg atccgggcca tcaagctgac
     1861 cccgccgcgt cccaacgctc tcgcctccaa tgcgacaaag cgacgacggc tctgttcatt
     1921 cagaacaggg gtcatctccc gatagcgttt gcgtgcggcc cgcgcctgtc ccgccagaat
     1981 catcccgaga cgctacgccg cgcgactcat cctcgtgaat ccggtcaaca taatcttctc
     2041 gatctgcgat atttatgact cagttattta tggatgggcc cttagctaac aaacgcggaa
     2101 cgcgtttgcg cggagatcat gctcaaacaa aaagatagag cgagatgacg attcgaagaa
     2161 aagtcatcac gctctagcgg gcgttcttgt tggcgggatt ttgcggaccc gtcggcgacg
     2221 tcaccaaggc aagcgatttc aattcgtccg gcttgaagac gaataatttg tcatgcggcg
     2281 tctccatcgc gtgcagccag acctggaggt cgacgcccat ctcgcggaga tagcgctcac
     2341 agcgggcgga gatgttctgg gcgaggctca tctcgtcgcg cggcggggtg ttggcggccg
     2401 attggatcgc cgcgatccgg tgcacgccga tgatggcacg atcgctggcg cggcgctcga
     2461 ggccgccggc aaacatcagc ggacaggacg acgcgcaata ttttccagcc tcgacttccg
     2521 ttgcgaattt tcgttcgcgg atcagccgtc ccatcgcctg cgcatccatc accgagccgc
     2581 cgggagaatt cagcaccacg gttttcacat agtcgccgcg ccgttcgatt tccgccgcga
     2641 aggcctgtga gctgccgggc gtgatggtgc cggtcgccat cagtctgccg ccgccagcca
     2701 gttcgaaagt catggcctgt gccagcgcgc cgtccggctg ctgtaggggc atcagccgct
     2761 tgtcaccgcc ggggagcaag ggcgcgagga tcgacgggag aacgccggga gaatccggcg
     2821 acagcgaatc ctgcctgatt tcagccggcg cggaagtcga gtcctccttg ccgatccatc
     2881 cagtcttgcc tgcaagatcg gccccaagca ccgcaatcgt caccgcgacg aggcagcgga
     2941 aaatccagcg taacaaagct tcgtcgaaat tgtccgccag ccaggcgagg aaacgctgcc
     3001 gcagggtaag agatgccgac atgatagcta cttggtgtac cgtgccgatg agagcgaggt
     3061 gacgttctct tcaggaaccg ctggctcggt ctcttcagga gccgtcggct tcgcttcctc
     3121 agcgactgca ggctcaggtt taggctcagg ctcaggtttg ggggccggct tggcttcctc
     3181 cgccgctcgc cgcgcctcct caacctcgcg tgcgatctgc aaggccgaca ccagttcgct
     3241 tgcggtgatg ttcgacgcgt tgaccaccgg cagtccctcg cgccggatcg ctgcgtggac
     3301 gacgcacagg atcagcacca gtactgccgg catcagatcg atcgagatcg cgccggccca
     3361 gcttggaatg aaatcgccgg catagcgcag caccgcttcg gcgggcgaca gcgtctgaaa
     3421 tctcgcaggt tcgacatgcc gcatcgccag aatcttgtcc gcggcgtcgg ccagcgaggc
     3481 cgcttgggtc gcgatcgaac tttccacctt cccgaccacc gcggtctgac ggtcggcaag
     3541 atcggaggtg cggccgccgg cggccggggc gatgaagccc gaggacagcg cggtcgccgc
     3601 gcgcttcacg gccggtgcca cggaggtttg ttgcagattg gcgatgacgc ccatcagcgc
     3661 cagcgactcg gttgcgaagg catcgctgcg cgcggcaatc ggcccgcggt ccgatatcaa
     3721 ctcccgcatc ttggcgaggt tcttgccacc ctgctcgtac aaggcggaca cgcgcttggc
     3781 cgaatcctgg acctgctggc cgaggctgtc gagttgtccg gacatctggg tcaacagctg
     3841 caccacggtg ccggatccgg cggtgcccgt gagcgagccc gcgcgttcgg cgtcggccag
     3901 gcgcgcaaag cgcgacgacg cgagttgaat atccggaagc agcccttggg ctgcgatcgc
     3961 attggtgttg gctgtgtcga gatcgcgcgt gtagtcctgc acggtaatcg cgagatgctg
     4021 ctgaatggcg gcggcccccg ccaaggccga ggcattgagc caggacgaca tcgccacgat
     4081 catcagcgag ccgagcagca tgcagccgaa catcaagccg cggccggtcg aactgaccac
     4141 atgcggcatg aactgcatca ggaataccca gaacgtgtag atgccgaccg agaccgcgac
     4201 cgagtagatc acggcggcga agaagaccgt cgtcgggctg ccgttgagca attcgcgaac
     4261 gccgagatag gtgtagacgc cggcggcaag cgcgagaatc gcggtggtca gcttgagggt
     4321 ggctccgagc cggctgacgc cattggcaag cagcacggag gtggaaacac gcgcggagga
     4381 ggactgcgat gtggaaggag aatttgagag catggcgagc cccgttgcgg ccatcaaaac
     4441 cgcgtcgacc aacgcccccg ttgtctgctg atcctttcac ggttaaggag aaattcggac
     4501 gagattatgt cggtgatgtt gcgcaacctg gaatgggttg atccgagacg acgttcggcg
     4561 gcatcgacga gcgtctatgc cgcaaccagg tccgcggtgg aaaccatgac cgcgatcctg
     4621 gcgaaggagc ttcgcggccg caacattacc gtgaatgtcg tggcaccggg accggtcgcg
     4681 atggacgtgt tcctgacagg caaatcgcag gagctggtcg atcgcatggc caagatgaat
     4741 ccgatggagt gcctgggtac gccggaggat atcgcctcca cggtggcttt tctcgtcgtc
     4801 ccgaaggcgg atggatcaat gcccaggtgc cgcgcaccaa tggcggcatg gtctgatcaa
     4861 ggcccgttca gccatgtcag catctcggcg ttgatttcgc gcggatagca atggctgaga
     4921 tcggcaatct ctcggtaggt cacgttggcc ccggccgccg atagcgcctg ttgagtgtgc
     4981 cgcgcgactt gcaccgggaa catccagtcg agccggccgt gaaccagaaa caccggcagg
     5041 ccgcgtaatc gttccgcgtc tgccatttcc gccatcagcg gatggaaggt ggctgcgacc
     5101 ggtgccagat gagtgaaggg cgaggcgccg tcgagcccgg tgacatagca gaacgtgccg
     5161 ccgtcgctca tgcccgtcag caacagccgt ttggaatcga tattccaccg gcttcctacg
     5221 tcatcgagaa tacgcaacag gttcggcgtg tccgtgttgt cgcccatcag cgcccaggtc
     5281 gggccggtcg cggtcggcgc gaccaggatg gcgccgaagc tgcgcgcatc gcgcaaccag
     5341 ctccacagga aattgcggcc gttgccgctg ccgccatgca gcgccatcac cagcggccag
     5401 gcgctgtctg gcgtgtaata ttccggcacg tagagcgaga atccgccgcg gctgccgggg
     5461 tcgtcgcggt cgtgcgtgac gcctgtgtgt tcagaccgcg gcgcggcaag gcgggccttc
     5521 agcgcgtcat cgtctcgcag cgccggatcg aggaagaaat cgctgaccgg cggaaacttt
     5581 tccgcgagcg ggtacagcgc ttcctgcgcg cgcgggaggt agcgcaacgc gcgaaacacc
     5641 gcgaccagat cgccattgcc gtgctggacc gcgcgcatgc ctgcgaaagc cgcgagcgcc
     5701 gcatcgctcg accgttccag cgacgcgcgc acgtccgcga attgttccgg ccgttcggtg
     5761 agacgcgtgc gaaccgtctg caacgcttcc tcgggcgcgc caatcgtttg catgacacga
     5821 tcgaagtctg gcgggttgag gtgacgcgca acgaaagcga gcgcctcgat cgattgcaag
     5881 agcggcggca gcacggccac gatgtcgtct atcacggcct cgctcatcgc gctttcccga
     5941 tttcagcttt cgtgcagact tcagagttaa tgcggtggat ttagcgaagc agatttaatg
     6001 gagcagatct aatgcagtct cttaacgaag ttctcttcgt gaaattctct tagtggagtc
     6061 ttggaccctt gagcaacttg gtgcggtcgg tcgaggccag caccacgtcg aaggtgacac
     6121 cctcatggtg gacggtgagc ggcacgtcga cgccggccgg cccgagcgac cagagcttgc
     6181 ggtagaaggc gctttggctc gtgatccttt cgcctttgac cgccaggatg acatcgccgg
     6241 ccttcagttc ggcgcgcgcg gcaggcccgt tgccggaaat tccgatcacc acgacgcggt
     6301 tgtcaatctc ggtcgagtac atcccaagcc acggtcgcgc cggccggttg acgcgtccga
     6361 acttgcgcaa gtcgtcgagg acaggcttga gcagatcgat cggcacgatc atgttgacgt
     6421 gctcggcctt gccggctcgc tcgcgttcga gctgaagcga gccgatgccg atcaattcgc
     6481 cggagttggc gatcagcgcc gtgccgcccc agttcggatg cgcgggatag gtgaagatcg
     6541 cttcgtcgag cagatactcc caatagccgg cgaactcctg cttggcggcg atgtggctcg
     6601 ccaccgagcg cgtgcgcccg cctgcgccgc cgaccaccac gcggtcgccc agccgcgccg
     6661 ccgccgaaga gccgagcggc aacggattgc ggtccatgcg accaagcgcc tgcaccagac
     6721 cgaagccggt gacgggatcg aaaccgagcg cgtggccctc catcacgcgg ccgtcgccaa
     6781 gatgcagcca gaccgtctcg gcttcggtga tcagataact gatggtcaag accagtccgt
     6841 cctcgatcac gacgccgttg ccggcgcgct cggtgcccag cgtttccgcg gtgaaggcgt
     6901 cggccgggat gagggaatgc aggccgacca ccgaggaaag ggcgcgatcg agatcgaaac
     6961 ggtagtcgct ggggcggggc tgaaaggcag gtggtacttt ccactcggtc acagagggca
     7021 tcgcggctct cctgttcaat cggcgcgact cggcgcgaat ggcggaattc cggcgccgaa
     7081 atccgtggcc gccgaagcgg tatttgttgc ttaatgtatc actattcgac cgatgcgcac
     7141 cagcgcagtt ggatggagtt gagtgttgtt tggccgttac gcaacctctg cgtcaatgga
     7201 gctcagccag cagggcacgg gcctcgcgga cgtcccgcgc ggtctcgccg ccctcgatca
     7261 tggcgagaat cggctcgatc aggcctcgaa tatcgctatt cgcccggctg ccacgccgga
     7321 gccgggcaag atcggtcgcc gcacgcagtt cgagcagacg cgcttgctga tttcgcgcga
     7381 cctcgattgc cttggtgaaa caggcttccg cgcgtgcgcg atccggctcg acctgcctga
     7441 gcgcaacctg cccgctgagc cggtgcaggt cggcgagcca gtattgctcg ccggattgtt
     7501 cgacaaaggc aaagccgtcc tgcagcgcag cttcggcgcc ggacagatct cccgcgatca
     7561 aaaatgcctc ggcaatattc gacaacagaa tcgaatttcc ggtattgccg cccgactggc
     7621 gcagcgcgac cagggcctcg cgaatggaaa cgatacggct ttcggtcggc gcgcgcatgg
     7681 cgctggcaca ggcgccaaga agaccggcca gcagccggat ctgctcgacc ttcttttcgg
     7741 cgcaataggc gctgagttcg gcactatcgc gctccaacgc ctcaatgtcc ctcagcgcga
     7801 actgaggcca aacggtcacg cagaatgtcg ccgatgcgat cgtggtggta tgaccatgac
     7861 ttgagagctc gctgcgcacc tgttcgctga cgcgtgccgc gctatcgagt tggccgagcg
     7921 acagtcgcac caggacttcg aacgacagga tggcgatgcc ttgatcccag ctgaaccggt
     7981 agctgagcgc cttttgccgg cgtgcgtcgc gataggtatg acccttttgc aggtttgcta
     8041 gcgcttcgcg aaaattcccg gcgtaaaact gaagcgttcc caactggcgg taaccgacca
     8101 gctggtaggc ggggtcattc tgccggtcgg cgacttcgat gatctgatgt gcgaaatcga
     8161 gcgcctgttt caggcgcgcg gtcagggtgg acgaggaaca aagtccgtac aggatggaaa
     8221 cgcgttgcag atcggtgcca atacctcggt cgaggagctg tttcgccgcc atcagcgccg
     8281 cgaacgtctc cggcgccgaa atgctcttta gcggcaccag cgcgttggcg cgggcgacca
     8341 acagaccaag ttcgagcgcg tcgcgttcag ttcccgcttc aatgctcgga atcagcgcaa
     8401 ggccaataga ggcgtgacgt tcggcttcga caggcgccga ctgcgacagc gccagctccg
     8461 ccgccgcaag ccagctccgt gccgcctggt cggaaagccc ggcttcggta tagtggtgtg
     8521 cgatgatggc gggcggatac tcttcgtcgg tgaggcgttg tttgagagcc caagcgatat
     8581 gagcatggat gcgttcgcgg cgacttcgca acagcgagcc ataggccgta tcctggacca
     8641 gcgcgtgttt gaacacatag ctggcctttg gcggggcgcc ccggatgtgg atcagctcgg
     8701 cttccatcaa ctggcgcagc gcctcgtcca gcgccgctcc cttgatcggc gataccgctt
     8761 ccagcagcgc ttgggagaac tcgcgtccga tcgcagcccc gatctgggca gtctccttga
     8821 tcggcgacag ccggtcgagc cgcgccgcca acgagtcatg caaggtcgag gggatcgcga
     8881 gcggcgtcag tgccgaggcc aacacatagg ttccgttctc ctcgcgcagc agtccggatt
     8941 ccaatacgct tttggtcagc tcctccatga acagcggaac gccgtcggtc ttcgccacga
     9001 tctggtcgag cacatccgcc ggcagcttct tgccggacgt catgcgctcg atcatcctca
     9061 ccgcctgatc ccgctcgagc cggttgagcg gcactgtcgt gacattgtcg cggccgagcc
     9121 aaggcgcggt gaactccgga cggtacgtcg tcacgagcag gatcggcaga tgacgcatct
     9181 gctcgaccag ccggttcatg aggtcgagcg acgtcggatc gatccaatgc gcgtcttcca
     9241 cgatcatcag gagcggtatc tgctttgccg ccccgacggt catcgcgatc agcgcagcga
     9301 tggtccgttc tctcaattcg cttggcgcca gatccagcgc cgggtagcgc tgtccggtcg
     9361 ggattgccaa tagcgaggcc aggtatggcg cgatatcgcg atttgagagg ccggtgctct
     9421 cgatcatctt ctccagcttg tcgagccgac tgcccgcggc ctcgccactg acaaagtccg
     9481 cggcgtacca gatctggccc atcaccggat ggaacgcgtc gttgatgtgg tgcggcgagc
     9541 attgatagcg tagcactagg tagttttgct tgccgatctg ctggcgcaac atcgcgagaa
     9601 cgcgtgattt gccgatgccg gcttctcccg acagcaacgc gacctggccc tcgcccttca
     9661 tcgcgcgctg ccaacgctcg tccaatagcg cgatctcctg ctcgcggccg acgaagggtg
     9721 tcagggtttc cgaacgcgag gcctcgaacc ggctgacatt ctcccgctcg cccgcgatga
     9781 gccaggctgc gaccggcacg tcgaaacctc tcagcgtctg cggcccgagt gccttgagct
     9841 caaaggtctt gccgagcagc cggcgcgtcg attccgacac cacgatggtg ttgggttcgg
     9901 cgaagccctg cagccgcatg gcgagatccg gcgtgtctcc aaccaggccc ctcaggcgcg
     9961 cttcgccctc gccgatcagt tcgccgacca cgacgaggcc ggtcgcgatg ccgacccgca
    10021 cttcgagcga tgtgtcatgg tggcgccgca gctccgcgac ggcatcgatc aacgcgacgc
    10081 cggctcgcgt tgcgcgctcg gcgtcgtcct catgcgcgcg gggatagccg aaatagacca
    10141 tggcggcatc gcccgtccac ttggcgacat ggccgtcgaa gcgcgctacc ggggccgcaa
    10201 ccgcgccctg aaacgcgcgg atcagatcgg ccatgtcctc ggggtcacgt tgcgccgtca
    10261 gcgccgtcga gccgacgaga tcgcagaaca tgacggtcaa ctgccgccgt tcagggtttt
    10321 cctgcggccg cgggttggaa tccggctgcg ccttcctggc gaggtgccgg atcgcgcgca
    10381 gcattctcaa ccggtgtcca agcggcacac cgatatcccg gagatgctca tccgtcagct
    10441 ccggcaagat atcggcttcg atctcgtgtt cccgaaacag gtcttcgtat tgatcgaggc
    10501 cgagactgcc aagccaggtc ccgacgtcca ttttccgttg gtcctcgtta tctggcaaca
    10561 ctaccgcgtg acgccggggc tagagcgcaa tgattttaga atgggtcgac atcgcgctcg
    10621 agcttattgt cttagcatga tctccgcgca aacgcgttcc gcgtttgttt agctaaggga
    10681 aaaccggtaa cccaccccgc atcaagtgcg gggcaggctt tttccggatc atgctctact
    10741 gcgccttggc ccgtgctcgt gacggcatga tccggaatgc gcgattctcc ttcagccatg
    10801 ccgcgcgctc atcgcgcagc agggtgcggc gaaccttgcc ggtatcgtca cgcaggccaa
    10861 tgcggacgat ctcgaagctt tcgggatgct tgtagcggct gagccggtcg gagaggaaat
    10921 ctcccatacc gtcggcgatc gcctgcgcat cggccgtctc gtcgagttcg aggatggcgt
    10981 gaacgcgctg accgaactcc ggatcgggca ggccgacggc gacacaggag cgcacgtccg
    11041 gatgcgcgct caccgcggct tccacctccg cgggatagat gttggcgccg ccgcgtagga
    11101 tcatatcggc gagccgatcg ccgagataga gatagccgtc cgcgtcgagc cggccgatgt
    11161 cgccaagtga ttcccagccg tcagcgcggc gcttcggctc ggcgccgaga tagtgatagg
    11221 tgctgccggg accgtcattg gggaggaaat agatttcgcc ggtctcgccg ggcgcgacgt
    11281 cgttgccgtc ctcgccgatg atgcgcaggc gcgcggtctc gccgatcttc ccgaccgatc
    11341 ccttgtgcgc gagccattcg acgccggaaa tcacgcacgc gccctgccgc tcggtgccgc
    11401 catagagctc ccagatccgt tcgggcccca gccatgcgat ccatttctcc ttgagccatg
    11461 gcggcatcgg ggctgccata tgaaacacga ttcgcaggct cgagaggtcg tagcgattgc
    11521 gcacctcgtc cggcagtgcc cagatgcgat gcatcatggt cggcacgaaa ttgacccatt
    11581 gcacgcggtg ggtttcgatg aggcgcagcg cctcttcggc gtcgaatttc gcaaggcccg
    11641 tcaatcggcc gcccgcgaac agacaagaat gcgacaggat gaatggcgcg ttgtgataga
    11701 gcgggccggg attgagcagc gaggcgcctt gcgggattcc gagcggcggc tcggatgcgg
    11761 tgtcgaccac ggctggcgca tggtcgagga tcaccttggg ccggccggtc gaaccgccgc
    11821 tggtcatcgc cttccaatag cgtgcgacgg ggctcgccag cggttcggcg gaaaaacctt
    11881 ccggcacgaa atcggccggc agcgcagtgg gcgcgttcca gtcggcctcg ccgccgacca
    11941 cgagcgcagg cttgagcacg tcgagcacgg cggcggcttc gccgcgcggc agccgccagg
    12001 acagcgacgt gggcgtcgcg ccaagcttcc acaccgcgaa gctggtctcg aagaaggcgt
    12061 tgccgttggg cagaccgatc gcgacgaagt cgccgggctt gacgcccttc tcggcaaagg
    12121 cgcgggcgcg ggcgttggcg ccgcgttcaa gctctcccca ggtgagcgtg tccgcgccat
    12181 ggctgatcgc gatcgtgtcg tctggtttgc gttcggcgta ccaccgcggc acgtcggcca
    12241 tcgggatcag catctcggtc ctccacgatc cggctgtttg tcgccgcgtt gtgttgcggc
    12301 aggcctcagc cgtccgcacc atcatcggag aagttgtgac gcaagacggg cgttgcgtca
    12361 gagaggcagc cgatgtgacc tcggacatgc aagatcccgt tccgatggac taagggattg
    12421 ccaagtggga cataaaggac gtcagtaaag cgaactatct cggcgattgc agcacgacgc
    12481 cgtagcgccc gtatattcgg tcgatcgtcc catcgccatg caaccgctca agagccgcat
    12541 cgatcgcctc gcgcagcttc tggtccgggc gcaccatgcc aacggcgaca ttccagttga
    12601 ggttcgtctc actttcgtcg cgatccagga tgcggaccgc attgtcccga cgcgtctgat
    12661 tgaaatagct ggcgatggtt ggcgtgaccg ctgctgcgtc gatctcgcga ttggccaggg
    12721 cttcaaggct gtcgacctcg aacccgaagg tcgatgtcgg cacgtgacgc tgacttatga
    12781 tcatcgccgc gatggatccg acctggactc cgaccttggt gtgctcgtca aggctcttga
    12841 acgacgtaag cttgctggct gaaggcacgg cgagtgcgac acccgtgcga tagtacggtt
    12901 ttgagatcgc caggttggtt tcgccctgtg cctcgcgatc cgcgatgaca tccatcacaa
    12961 tgtcgcagcc ggcgctgcgc agctggtatt gggtgatgat ccagtcgagc cttagcgaga
    13021 cgccaagctc gcgcgccagg gcctgagcca gttcgacctg gaatccagga gggtcgcctg
    13081 ccttgctggc gaatggaagc gagttggggt gggcgcacag cccgatggtg ccgtatgagc
    13141 ggatcgtctc gagagaccgt gcctgtgccg gatcgatcaa gccgatgacg ccagcgacga
    13201 tgagggggag aatcgctttc atgccgtttt ctcaggtggc ggatggaccg gatcttgcgg
    13261 cctcatcctt cacgcggttt caatgaccga atgtacttga tgatgtcttc gatctgcgcg
    13321 tcggtgaagg ttgcgccgaa cgcgggcatc gcgccgttct tgccgttctt gatacggtcc
    13381 cgaataaagt catcgcttcg gggcgagtcc atgagctgcg gtcccctgcc ggcagcgcgt
    13441 cccccgctgg aatgacaaaa gccgcaggtc gatgcgaaca gctgttcgac atcgaatgcc
    13501 ccgctgtccg gtgcattggc cgcgggctgt gccctggcgg gcagggcgca tatcaacgag
    13561 acggccagca cggcggggag tagtgcctta acgtccatgt ccaatttcat gatgttctgg
    13621 ttctgcagcg gcgcatcggc ccgtttgaat gcacggggag cgagcttgct cgctccccgt
    13681 caggcgatcc aaatcgctta tttcaggctg tagacgatca gcgcgccatc atcgcgcggc
    13741 atgctcttgt agacgccgcc gaacgtcgcg gcgtaatcgt ccgacagcat accgccgaag
    13801 ccagcggtca tggcgatata ttgcttgccg ccgacggagt agctgacgat gccaccctgg
    13861 tggccggtgc cgttggaatg attccacagc tcggtccccg tttcggcgtc gtaggcgtga
    13921 accacgccgc gcgagtcggg cacgaacacg agattgccag cggtcgaaag tacgctggca
    13981 atcggcggct cggggaagcg aacctcccat ttcttggccc cggtcaccgg gtcgcgcgcg
    14041 tcgagatgac cataaatctc gccgcccgga ggaggtgcaa tcttgaaatc ggcgccgatg
    14101 ttgagttggg cctgtggggc ggtcaccggc gtcgtcttca cgacgtcaag cgtcatgcac
    14161 cattcctggc cgatcttgta gtagaggccc gtcttcgggt tgtatgagcc ggagttccag
    14221 ctgacgccgc caccaatgta cgggcacagc ggcggtgcct caactttccc ggctgtgaag
    14281 tcgcggcgac cgatcagctc gcccgtcttg gggtcgatat ccttgacgaa attgatgttc
    14341 tgaacaagcc gccagacgtt cttcacagcg agattgcggt cgtagacgaa gatgaagccg
    14401 ctcttgttgg gatgcacgat gagcttctgg ccgtcgcgat cgagcatcaa gaactcgccg
    14461 accgcgctgt cgaagtccca ggcgtcgtgc ggcagctcct gatgatagga tttcaacttg
    14521 ccggtatcga catcgagagc gatcaccgag ctcgtataga gattgtcgcc agggcgggcc
    14581 ccttgcgtct tatagtcgtt acccgaccaa tcgtagagcg gcgccgggtt ggcggtgccc
    14641 cacaaaatgg tgttggtgtc ggaatcgtaa gtgcccggca tccagccgcc gcctccgcca
    14701 gtgcgccaag agtcacttcc ccaggtcttc atggcttcgt cggtgccggc gacggtcagg
    14761 aattcccatt tcttcttgcc ggttgctgca tcaacaccga atatcgggcc gcggcccggc
    14821 cattcgccgc cctgcgagcc gatcaccacc gtgcctttgg cataaagcgg cgcaccggtg
    14881 aagccgaccg tcagcttctg ggaatcgatc agcttggtat cccagagcgg cttgccgctc
    14941 ttcatgtcca gtgcgatgag ccggccgtcc atcgtgccga catagacctt gccctcgcca
    15001 agcgcgacgc cgcgattgta gggcgagtgg gtctgccggg cgatcaacgc ttcgtccagt
    15061 tccgggaagt acgaccagat cacctcgccg gtggcgccgt tcagcgcgaa ggttcggctg
    15121 taggatcccg tgtagtagag cacgccatcg gccacgagcg gcattgactg caggccacgg
    15181 gtggatcgtc caggaatatg gatccaggca acgcccagat tggcgacgtt gcttgggttg
    15241 atctgggcga gcggactgta gtgatgagac ttgtaggagc cgtggtaggt caaccagtca
    15301 ttctggccgc cggcctcgat gcgcgcggta tccactgact gagcaaacgt tgacgatgcg
    15361 gcgaatgtcg ctgcaatgac aacggcaaca ggtaaggcaa gataattcga ctgtttcgtc
    15421 atgggcactt cctccggttt gaattttttt atgcgtagtc gctgcattgc cgggccgctg
    15481 cgcacgatcg tatgccgacg cgatcgatct cgtgccgcgt tccctacaat tcagatttcg
    15541 ttttcatcaa gacgccccca tcctcgccca agtcatggta tcaagtcatg gtatcaagtc
    15601 atggcatcaa gtcatggcat cgggcttgtc gccgagtctt tattttgaag ggaacccagc
    15661 cattttgtcg cttttttgac cctcagagag ccggcgttca tgctcaatcc ttgagcgcgg
    15721 ctgaacaggc gccggctttc acgctattga attgcaagct attgaattgg ccgcgcagcg
    15781 atttttccct ttggcgtttg ccgagatgtt ttgccggcgt cgctgcggcg agtttggaat
    15841 atccatcggt tcattcgcga agaacgggca ccgcagcgca ccaaaccgta acagatcgtg
    15901 tcaccgcttg atagtgatgc tgcagcgcca acttgcgatg atgatcgaaa actcgaaaac
    15961 tcgaaagctg gcgccattcc aacactcgcc gatgggaccg gcgtcttgtg cttgattcat
    16021 gattgagcga gcgtgccggt cggccaccaa atcgacgaga acgctcgtgc cttttttgcg
    16081 ctgcaaattc aggctgatcg ggcccgtcgg gtgcggcttg gagaaatcgc cagctttgcc
    16141 cgacgaggca aatcatagct acaagctcgc tccagcgatt ccgcgccggc gggcctggat
    16201 catgtccgag cactgattgg cagaacccac gggaagcgcc gtcgtttctg acatgtagtg
    16261 caacaaagcg gacgtgtcgg gggcaagcgc ggcgctgtcg gctttcaaca aggacgtgcc
    16321 acggaggaac caggcggggg gtcattgatt atcaaatcgt attggccaag tagcctgccg
    16381 cgttgcgctt gatatttcga tccaagagtc gacccatgaa cgaacccgat aatgaaatgt
    16441 ttgccgccgc tgccatcgag cgggcacttg gtatctatca acagctgcgg gagcgcgacc
    16501 ggtcggtaat cttgcaggca cggaaaattc tcactcagca tatttacggc atgttcgata
    16561 agggggagca cgacgagaag cggctagttg taagcggtct cgcgcgccta acatcgattg
    16621 agcgcgacca cgcgataaaa tccgctcatg atgttccggc tgcttcaact ggcaaacgaa
    16681 gacgtcgcgc atctgcctga tgactggagc cgccgggaat ggccgtatac ggcaccgcgg
    16741 cgaagtcggc gcgttcatgc cggcccatct gatcagggag caagcggatc aggccggagc
    16801 gggtggcatc ggtcgtgccg ggagcgggca ccatcgagct catggccgac gcaatctctc
    16861 ggagaaagtc ggaatgagtt tctaatatgc aactacaccg caatcctatc tacgtcggaa
    16921 tcgagcggga cccgatgcat aatgcccacc gagcctagct cggtgggcat tatggtgtct
    16981 cctaaactgc gcggctcaat ggcgctatca gtagtccgga ccgataccga agctgaaccc
    17041 cggtccacgg acaccaacgc ccggcccacg gtggtaatag ccgcgatcgt agtcgtcgta
    17101 gtatcgcggt ccatagtaac tctctctgcc tccgatgatc acgcgacgat caccccgggt
    17161 attccagcat tggccagcgt cattacagac cagcctggcc tggtgaagca gagccgagtt
    17221 gtctccggtt tgaatccggt cggcggcata ggcgctcgtg gctaggaggg caccggcgcc
    17281 ggcgagaaat gtcagtgtca gttttctcat ctgcgtctcc tctaaggtga gcattaagga
    17341 aaacgtctta atcaggacac cgttccaatg aaattggtca taggtatcga tgaaatttcc
    17401 caacggaacc taaagcgaga gatgccgctc gatcgatcat gcgatctggg ttggaaacga
    17461 gcggaacatt tttgaggccg agtcattcgt tttcaatgtg gtggccggcc aggtgttcca
    17521 tgctggaggg tcaagagcgc ccttttgcga tccggaaccg ggcgcgcgca aaacgctaga
    17581 cgaaggacga agatgaccat gagagtagcc gaaaccatga cgcgaggcgt ctttatcgta
    17641 agcccggaag aaaccatcca ggaggcggca atggccatgg cgggaatcga cgcgggcgtc
    17701 ctgccgataa gcaacggtga tcggctcgtt ggcatgatca ccgatcgcga tatcgcaatt
    17761 cgcggcgttg cggagggaaa gggcccagat accaaagttc gcgacgtcat gacgaccgag
    17821 ataaagtact gttttgatga ccaggaggtc gatgacgtta ttcgcaacat gggtgaaatc
    17881 caggtccggc gcctgccggt ggtcgatcgc gacaaacggc ttgtcggcat tttgtcgctt
    17941 ggagacgttg ccgccaccgc cagcgatgga aaggcgggcg aagcgttgag caaaatctcg
    18001 cgaccgggcg gagagcacac ccaaaccggc tgaagcggat gaaatcgatc catcggtgag
    18061 atcagccggg cgcttttgga atccttgccc gcgcaatcag gcttgatcga aggccaatac
    18121 ctgtccgtct gccgccgcaa tcaaccggac gccctctcgc tcggcggcga ctccatccca
    18181 ccaatcgccg acgaaagata tcacgaagac ggaagatatc acgaacaagc gatgcggttg
    18241 gagtgaactc tgacctaggc cgcgaaagca agggagggtt ccttccaccg tctctgtatc
    18301 gcgaggtcgg ggtatcgcga ggtcgggaac ctttcgcgtc gttttgattt gccctcacag
    18361 aacgggaaat aacagcaaac cgatccagcg atgaccgaca ggaatgaagc agagccgccc
    18421 aatgctcgtc agatgctggg tcgccggctt tggctagtgt agggagacat ctcatgaccg
    18481 aagggcgtac ccgcggcagg atgcgtcgcg ctctcgctgg ttctatcctt gcgattggaa
    18541 ttgttggatt cggaacggtc gcctggctgg tcggatcgcg tgtctcaacg tggagagacc
    18601 tgtatatctg gtacaacccc gcggttggat ttcaatctgc tgcgctgccg gtgggctttg
    18661 aagatctggt ggaacgaatc aaaccaacag ttgtgggcgt ccgcgcgaag gtggagcgag
    18721 acgccgacga agaggatcag cctgccggtc acggcgcgcc ctccagtcgt tccgatttgc
    18781 ccaagggcaa tgtcggcgcg aaccagcccc atgtcgccac cagtcagggg tcaggcttct
    18841 tcatatcgtc ggacggatat gccatgacca ccaatcacgt ggtcgagcgc agcgaaaaaa
    18901 ttgaggtcac caccgacgac ggaaagattt accctgcgaa gctggttggt gctgacccga
    18961 agaccgacct cgcacttctc aagatagacg gcggaaacga attcccggct gcccggatag
    19021 ctgacaaggc ccctcgaatc ggcgagtggg ttcttgctgt cggcaatcca ttcggactcg
    19081 gtggaacagt gacggctgga attgtctctg ctcgcgcgcg cgacatcaag attgcagctt
    19141 acaacgactt cattcagatc gatgcgccgg tcaatcaggg caactctggc ggaccgacct
    19201 tcgatgtcgg cggcaacgtc attggcgtta acagtgcaat attttcgccc accggtggct
    19261 cgatcggtat cggctttgcc attccagcgg aaacagtgaa gacgatcgcg gacaggctca
    19321 aagacaaggg caccatcagg cgcggctgga tcggcatcga ggttcagtcc ttgagccccg
    19381 aaatcgtcga gggtcttggc gtcaataaat cggtccatgg cgtcctggtc gccgaaaccc
    19441 aaccggacag cccggcagca aaggccggca tcgtgccggg cgacgtcatt acgtccctcg
    19501 cgggccaacc gaccagtgac gatcgtgact tggtaaaaag ggtcggcgat atgtcccctg
    19561 gaaaatcaat cgagcttcgc ctgatccatc agaacgagga aaaagccatt agcgtaacgc
    19621 taggcgaact tcccggtcct cgcaatgaaa gcggcttgac taccgaaaag caaaaatcac
    19681 ccgcggcaga tggcaatcag gcaaatatag gattgacctt gatgcctgca gacaagctcg
    19741 gtctcgttgt tggaggcgtc gtcgtgactg aacttgatac cgctggtgtg gccgctgaaa
    19801 gcggtctttc gatcggagat gtcattctcg acgtaggcgg caccgtgatc aaaacacctg
    19861 tcgacttcta caaggcgctg actgaggttc aaagtcgggg cgggcgcgtc gcattagcgc
    19921 gagtcaagtc aaacgacgca acgcgattta tcgctattcc tgtcggctga tttcttacat
    19981 ccgctccagc tctgctactc gccgcgtccg ctcttttcca tggagagccc ggagtgatgg
    20041 tgtttgggct tttcgcggca attccggctg ctgtctgcga agattctgcg caggtcccgc
    20101 tcctccgcgt ccttggaact taatcggccg atgagtccaa gcaccgcgct tcgcctcatc
    20161 gacgcttgcg atttcagcgg cccggccggc tcagcgggct gtgagaaacg tggcagtctt
    20221 ttgtgctttt tgacactctt atgagtccca cacaaatatg agtcccacac aaagcagccc
    20281 caaccctcac ggatcgagct cggtgtccca atagagatag tcgagccagc tatcgtgcag
    20341 atagttcggc gggaacagcc ggccgttgcg gtgcaattga tgcacggttg gcgcgaaggg
    20401 ggcttgcctc ggaaacatct tcgcctgctg cggcgtcagg tttcccttcc gcaagttgca
    20461 cggcgagcag gcggcgacga cgttttccca ggtggtctgg ccgcctttgc tgcgcgggac
    20521 gatgtgatcg aaggtgagat cgtcgtccgc cgaacaatac tggcagacga accggtcgcg
    20581 caggaacacg ttgaagcggg tgaaggccgg gtgggtgctc ggtttgacga aggacttcag
    20641 cgacaccacg cttggcagct gaatttcaaa cgagggactg cgcaccgccc tgtcatagtg
    20701 ttccacgatg ttgacgcgat cgaggaacac cgccttgatc gcgtcctgcc acgaccaaag
    20761 cgagagcggg taataactca gcggccggaa gtccgcattc agtacgagta ccggccaacc
    20821 gccttgtgag acatgtgcgt tcaaataacg ctcctgactc ccatatctgc tgcgacgcag
    20881 cttgtattga catactacat gcagcgtgac gggattgtga agagcttaga atagcttatc
    20941 ccgcaatcgc ggtccgcgcc gaacgcgcat ccggcccgcg cccaagggct gaacaagagc
    21001 agtggaatca gggggagaat ggcgtatgcg tcaatggttg gcgctattga ttgcggcgtt
    21061 tatcggtctg ggatcgagca atatttgcaa ggcgcagggc tatccggccc ggccgatcac
    21121 catgatcgtg ccatttccgg cgggtggcgc caccgacacg gtggcgcggt tccttggtga
    21181 gaagatgagg gccattctgg ggcagcccgt cgtcattgag aatgtcgcgg gcgctgccgg
    21241 cagcttgggt gtcggccgcg cggtccgctc tgccgctgac ggccatacac tgagcatcgg
    21301 aacctccacg acgcatgtgc tgaccggcgg cctctatgct ctgccgttcg atctgctcag
    21361 ggatttggaa ccggtgatcg aaatcggcag cgagccgctc ttgatcgtcg caaagaaaag
    21421 cctgccggcg gacgatttga aggggctgat cgcctggctc aaggccaatc ccgataaggc
    21481 gtcggtgggg atcgccggtg tcggcgccac tggccatctc acgggaattt catttcagaa
    21541 ggagaccgga accaggttcc agtttgtgcc atatcgcggc aatgccccgg ccatgcagga
    21601 tctcctcgcc gagcagattg acttcatgat cgagccgtcg tcgaacttca aggcgctgtt
    21661 cggggcgggc agcgtcaagc cgttcgccat cacgggcaga acgcggctgc ggtcgtctcc
    21721 cgacattccg accgccgacg aggcgggact gccgggcttc catgcgtcgc tgtggtatgg
    21781 gctctgggtg ccgaagggca caccgaagga gatcatcgcg aagctgaacg ccaccatgag
    21841 ccagattctg gccgatccgc aggtcaagca gcgcttcgac gaattaggca ttcagatcac
    21901 gccgctcgat cagcaatcgc ccgaggcgct ccgcgcattc cagaaggcgg aggccgaacg
    21961 ctggtggccg atcatgaagg cctcgaacat caaggcggag tagggcgctg gcccgtcatt
    22021 gcgagcgaag cgaagcaatc cagagccaag caggaagtct ggattgcttc gcggagcctg
    22081 tcatccggcg gcgcttttgc gccgaccggt tggctcgcaa tgacgaagct cgctacgacg
    22141 gactactcgc gaatcctctc aagcttctgc aagcagcgga cgccgcgaac ctccggcggc
    22201 atcggcgtgt ccggaaaact cgatttccag ttggtgacgc cgacctcgcc gacatagatg
    22261 tcgcctttgg aatcgagcgc cagcccgtgc ggcgccagga atttgccggt ctgaagcccc
    22321 gggccgtgct cgccgccgag ccgtgcgatg cggttgccct tggcatcgac gatggtcagg
    22381 cgcgggccga gattgggcac tttcagattg acgggcatgc caggaccgag ttcgccgacg
    22441 atgaaattcg gatgcttgcc gccgcagcaa tgcagcgcgc agggccggtg cagattgttc
    22501 cactgggtct cgtatctgcc gttgccgtcg aacacctgga cgcggtgatt ttcgcggtcg
    22561 gcgacataga cccatccgtc cgcatcggtt gcgatgttgt ggacgatgtt gaactggccg
    22621 ggatcggtgc cgggctcgcc ccagcttttg agcagcctgc cgtctggcgt gtatttgtgg
    22681 acgcaggcgt tgccgtaacc gtcggagacg tagatctccc ccttgggcga cagcgcagta
    22741 tgcgtgcagc ggtgaaacgg ctcgccgctc atgaacggcg ccggcttgtt cggaatgccg
    22801 atcgtcaata gcaccctgcc gtcggtcgtg catctgcgca cggtgtggtc gccgtcgtcg
    22861 gtgcaataaa gattgtcgtc ggcgtcgatg tagagcccgt gggcgcgcgc aaacaggcct
    22921 tcgccccaac tcgtgatgaa attgccctcg cgatcgagca ccaccatcgg atgggcgccg
    22981 cggttgaaga catagacgcg gtccttgctg tcgaccgcga ccgcgccgac atctcggaac
    23041 tcccagccgt cgggcagctt ggcccagtct tcgacgacgc gatatcgatg ctcaccggtt
    23101 cccaaaacta cggacatgtg ttgctccagc atttcatcgc ccggccagga cggcgtcggc
    23161 gattttctcg gcggtcatca gggtcgggaa atttgtgttg gcgcacggca cgaccgggaa
    23221 gatcgaggcg tcgaccacgc gaaggccctg cacgcctttg accaggccgg ccgtgtcgac
    23281 cactgccatc ggatcgtcgg cgcggcccat gcggcaggag catgaggcat gccagacgcc
    23341 gatcgcggcc ttgcggatga aggcttcgag cgcatcgtcg tcggtcatca cctcgtggaa
    23401 attgaagccc tcgacgacaa accggtcgat caggaggctg cgcagcgcag cgggaccatc
    23461 gaggagcttg gcgacgatcg cggtcacaac cttgttcttg gtgttaacga cgccgatctt
    23521 gcgcacgcgg tcgctgtagc tcgccggaaa ggggtcggtg gtcaccgcct tcaaagcgtc
    23581 gctcatctgg agggcggcca tgcggtgaaa g
//
LOCUS       DATNOI010000287        13554 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1262039,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000287 DATNOI010000000
VERSION     DATNOI010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13554
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1262039"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VN968_11160"
     CDS             complement(<1..880)
                     /locus_tag="VN968_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXL29834.1"
                     /translation="MNFQLRPHLMTAERRLPFECVALLLQGGGALGAYQGGVYEALAE
                     ANIHPDWIAGISIGAINGAIIAGNPPHSRVDRLREFWTQVTSSTPWDWSNNPFVDLAR
                     SDHTRNLLNQMSAGLAASFGAAGFFSARPLMPWLQPGGTLGATSFYDTNSLRKTLERL
                     VDFDRINAGMTRFSAGAVNVRTGNFAYFDSTTHTIRPEHVMASGALPPGFAAVEIEGE
                     QYWDGGLVSNTPLQWVIESSPRRQDTLAFQVDLWSARGQLPRNMAEVATRQKEIQFSS
                     RTRASTDQFKNVHRVQR"
     gene            1166..1426
                     /locus_tag="VN968_11165"
     CDS             1166..1426
                     /locus_tag="VN968_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29835.1"
                     /translation="MISKTRRPCIAVVAALLCATTASAQENHGTPEQRAACAPDAFRL
                     CAGYIPDPTRVEHCLRQNKSDLSDACRSVFEQSAGPVASRVK"
     gene            1516..1953
                     /locus_tag="VN968_11170"
     CDS             1516..1953
                     /locus_tag="VN968_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29836.1"
                     /translation="MDMFYIPAIASFSGSASGTLASIVTGWVTQRRKDRIRHSLRATS
                     QREELYKSFIEEASRLYADALVNDKSEVSKLVDLYALIARMKILSSDAVIEAAEKAGR
                     VILETYLSPNRTFVDLPDLINEMDPLRDFSEACRRELQSAPQR"
     gene            1966..2421
                     /locus_tag="VN968_11175"
     CDS             1966..2421
                     /locus_tag="VN968_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29837.1"
                     /translation="MPLLQYFGWVGSLLLAVLLAANWCFPAPVAPLSDVSLDRKIDIR
                     IHTDHKWPERVVFDTTDAMPAQKASGEAGIGGSEPAVVAERQPFDAFAQMAAIPVRPY
                     FRPPCSAGPAAAREDSRFGKAALSQSRAHLAARKDLTFPSRLHKPPGKS"
     gene            complement(2378..3049)
                     /locus_tag="VN968_11180"
     CDS             complement(2378..3049)
                     /locus_tag="VN968_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL29838.1"
                     /translation="MTKRNDLKRPRPGRPPKDLAGDVKARILDAAQRVFLKRGYQSAS
                     LDEIAETAPVSKPTIYAHFPGKEALFEAVVARVIDGLTNFEGFEPKGRAVQDKLASLG
                     IEVVERFIEETIGVTRATIAEADRFPALSRHVHEAGRDRAAAAVSHVLNDATHTLSRG
                     SKGPFGPKRSLTTAQIFMDLILLPMLMRALMGEGAKDLRSELPTFVRERVSFFLAACE
                     ADWGK"
     gene            3181..3606
                     /locus_tag="VN968_11185"
     CDS             3181..3606
                     /locus_tag="VN968_11185"
                     /inference="COORDINATES: protein motif:HMM:NF019205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HXL29839.1"
                     /translation="MSDSPPYPPALEGRTLLDELNHRIKNELASVINLVTFKAVWTDN
                     ADAKEELSNVVDLLHQHVEVHSILTMPDRDALVDAAEQLRKLGLAMSRCKLDRMNIRL
                     MLSADALPLESERCWRLALAIYELVNERGPPRLLRWPGR"
     gene            3623..3940
                     /locus_tag="VN968_11190"
     CDS             3623..3940
                     /locus_tag="VN968_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29840.1"
                     /translation="MRAGSWVNCHVSDNGSGAGRIKLGRGLRIVGDLVASLGGRIDRT
                     SGTTWNSFALDFPLTQREQRANRVVVARRPRTGRRPRTMPSVASVSAASEPANPIAVQ
                     PQL"
     gene            3959..4531
                     /locus_tag="VN968_11195"
     CDS             3959..4531
                     /locus_tag="VN968_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29841.1"
                     /translation="MSTRTLLTLTASLVLGAAIAAPSAALAQFGPPPGPPPAFAGPPP
                     GLGAGGPPPGLGAGGAPLGGVPAGPVAGFPPRGPAGAPPRDIAGGPRLEGAGGLRGLD
                     RGGQANFRGVEGHAAAYSANSYTRNSYNSYGRGYGYGHGSRYWPYAAAAAGAYAYGSS
                     YTSSDEGCYYVSTYRRYGTRRVLVCHGDGD"
     gene            4731..5798
                     /locus_tag="VN968_11200"
     CDS             4731..5798
                     /locus_tag="VN968_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXL29842.1"
                     /translation="MRAALALVLLAAPLSACNDPATTVERAAFVRTEIVRPRDGQASL
                     TLTGEVQARFSADLSFRVNGRVLARLVDVGAHVNVGDVLARLDPAEQQADLDAATAGV
                     AAAESQLRVAKATFDRQTSLISSGFTTRVAYDQAQEQLRSAESTLETAKSQLGQAREA
                     LGDTELHARASGVITARNLEVGQVVQSAQSVFTLAQDGDRDAVFDVPESFFSGDVESR
                     RVSLTLVSDPDVTAVGDVRELSPAVDPKSLTVRVKVAIQNPPAAMTLGSAITGTAGTK
                     AAREIALPWTALMATGSKPAVWIVDPRTKTASLKPVSVAGYEARAVLIKGGLEAGDRV
                     VVDGGKLLSSGQPVTYDEDRS"
     gene            5795..6871
                     /locus_tag="VN968_11205"
     CDS             5795..6871
                     /locus_tag="VN968_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXL29843.1"
                     /translation="MMTRALALGSIFALALVLTGCKQETKGEEPVRPVLSHVLKPTAP
                     GNTQAVGTVQPRYETNLGFRVLGRLIARPVNVGDLVAEGQTIAAIDPTVLELAVRSAK
                     ADLSKAQAVLENASASEERKRILIKTDATTKQTLDDAEQMRAGAQASTARAQANLTKA
                     IEQLGYAQVKADFAGVVTAVGAEVGQVVSPGQRVVTVARPEIREAVVDIGEDFPVPLK
                     IDLPFAVSLQLRPALQVQGQIREIAPQADSVTRMRRVRISLTDPPESFRLGSTVTARP
                     NNSENSVLRVPTSAVLTRDSQNFVWVVDPLTGTVSLHKVDLAEDEGGLRVTDGLPEGA
                     RIVTAGIHSLKPGQQVRFEQDLAP"
     gene            6868..10008
                     /locus_tag="VN968_11210"
     CDS             6868..10008
                     /locus_tag="VN968_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXL29844.1"
                     /translation="MISFNLSNWALGHRSLVWYFMLAFMAAGLFSYLQLGRQEDPDFT
                     IKTMVIQAQWPGASPEEMTRQVTDRIEKKLEELESLDYTKSVTVAGQTTVYVYLRDTT
                     KAADVKPTWVRVRNMIADIKGDFPQGVIGPGFNDRFGDVFGNIYAFTSDGLTQRQLRD
                     EVEDIRAKVLTVPDVGKVDILGAQDEVIYLEFSTRKIAALGLDIHSIMASLQGQNAVA
                     RSGVFQEGPERISVRVNGQFTSEASLKAVNLRINDRFFPLTDVATITRGYTDPAKTLF
                     RYNGEPAIALAIGMKSGANLLHFGEALKQEMSKVIADLPIGVGVHLVADQPVIVEHAV
                     SGFTEALFEAVVIVLAISFISLGMRAGLVVAIAIPLVLAITFVVMAYADISLQRISLG
                     ALIIALGLLVDDAMIAVEMMVARLEVGDPLEKAATHVYTSTAFPMLTGTLVTVAGFIP
                     IGLNSSNAGEFTFTLFVVIAVSLIVSWIVAVLFTPLLGVAILPAKMKGHHGEKGRVAQ
                     MFARLLLVCMHHRWTTVGVTVAAFLIALFGMKFIPQQFFPSSDRSELVIDWSLPQNAS
                     IADTNAQMARFEREKLQGNGSVEHWSTYVGTGAPRFVLSFDVQTANTWFGQQVVVTKG
                     GIKARDQVKAQFEDYLKKTFPGTDTYVKLLEVGPPVGRPVQYRMSGPDIAKVKDLSQQ
                     LAGIVRSSPDLGNVVFDWMEPARVVKVDVLQDKARQLGVTSEDIATALNSVLEGTPIT
                     QVRDSIYLVNVTGRATAAERASIDTLRDLQLPGVGGQSVPLGALANLRYELEQPTIWR
                     RARIPTITLKAATVGNAQPKTVVDQLAPKVAEFAKELPAGYSVKIGGSVEESAKSQAP
                     IVAVVPLMLFVMATVLMIQLQSFHRLFLVFAVAPLAVIGVVMAMLPSGAPLGFVAILG
                     VLALIGILVRNSVILIVQIEDLKKEGRGDWDAVVEATEHRMRPILLTAAAASLALIPI
                     AREIFWGPMAYAMMGGIIVGTLLTLLFLPALYVAWFRIKEPEPAMKDIQKNPIPWESR
                     TDLPADAPHRHEMENA"
     gene            10353..11120
                     /locus_tag="VN968_11215"
     CDS             10353..11120
                     /locus_tag="VN968_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29845.1"
                     /translation="MTTLVETPTQIAARAGTQYFYFYMALSCTAVAFFGFAPTYWLPM
                     AARTFKSSPIVHIHGLVFFCWTLFFCFQTWLAASGRIANHRSIGMIGISFATAMTIFG
                     VLASINQMKTAAAVGLKDEGIAFAIVPLSGILFFAVAFSLAIAAVRRPAQHKRLMLLA
                     AISLLDAAVARWFLTFLAPPGPPGPPPVAVTLLPALVAYLLLAAAIVFDWRTRGRPHP
                     VYVFGGAALVAIKVLNLPISATSWWHSFAGEILALAQ"
     gene            11219..12013
                     /locus_tag="VN968_11220"
     CDS             11219..12013
                     /locus_tag="VN968_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29846.1"
                     /translation="MTPEVRMDWFGWQLPKDPAADKSAAFGPQAILQELGKRVPQYLD
                     DIDQGRLIYPACKRTPSDPHGDIRSIWDHTRLEAARYVSMVPRREFEPLAEPARQPDV
                     LEAYLRQRPHEDTVIEFTGSTMGDLAIAIIAGFNWLNHCAALAGSDRDKFSGTLSNFR
                     KVAGLAWQWWAIEGAGERCSQMLAEGEKPPLMLNLLWTEYTRLAKEVAAAAFFGSSIE
                     QAIAGRGEVLKSEFAGRPEELDSALGELAETMASFERARDPDDLIG"
     gene            complement(12213..12410)
                     /locus_tag="VN968_11225"
     CDS             complement(12213..12410)
                     /locus_tag="VN968_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29847.1"
                     /translation="MVYKMIAERGNETVKSERTSSLITMARARVWASEGWQVTITNDE
                     GKVFAPAEFETLLAAQPARGG"
     gene            12742..13422
                     /locus_tag="VN968_11230"
     CDS             12742..13422
                     /locus_tag="VN968_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HXL29848.1"
                     /translation="MAAPSDSVQTVDQAGPSDEPFGLSTETVTRGTALEKWLNVEHQI
                     DNDRLVLKTCQENRASCQSPAALQFLAIVDIGRNLEGRARLGEINRAINLKLKPKSDL
                     ALYGEEDVWTSPLATFAIGAGDCEDYAIAKFVALQEAGVSPDDLRIVILRDDHREEDH
                     AVVAARLDGNWLMLDNRHMVLVEDQLVRRYRPVFLADRDGVKLYSDTPALAGGSRGYE
                     RAIGQIRR"
BASE COUNT         2500 a         4194 c         4132 g         2728 t
ORIGIN      
        1 cacgctgcac ccggtgcacg ttcttgaact ggtcggtgct tgcccgcgtt cggctcgaaa
       61 attgaatctc tttctgacgt gtggcgacct cggccatgtt gcgcggaagc tggccccgcg
      121 cgctccagag atcgacctgg aatgcgagcg tatcctggcg gcggggactg ctctcaatca
      181 cccactgcag cggcgtgttg gacacaaggc cgccatccca gtactgctcg ccctcgatct
      241 cgacggcggc aaagcccggt gggagggccc cgcttgccat gacatgctcc ggcctgatcg
      301 tatgcgtggt gctgtcgaaa taggcgaagt tgccggtcct gacattgaca gcccccgcgc
      361 tgaagcgcgt catcccggcg ttgatgcgat caaaatcaac caggcgctcc aaagttttcc
      421 tgagcgaatt cgtatcgtag aagctggtcg ccccgagtgt cccgcccggt tgcagccagg
      481 gcatcaacgg gcgcgcggag aagaaccccg ctgcaccgaa cgaggcagca agaccggcgc
      541 tcatctggtt gagcaggttg cgtgtgtggt cgcttcgcgc caaatccacg aacgggttgt
      601 ttgaccaatc ccaaggcgtg ctcgaggtca cctgcgtcca aaactcgcgc agccgatcga
      661 ctcgagagtg cggcggattg cctgcaataa tggcaccatt gatggctccg atcgagatgc
      721 cggcgatcca gtctggatgg atattggctt ccgccagcgc ctcgtagacg cctccctggt
      781 atgcccccaa cgcaccgccg ccttgcagaa gcaaggcgac gcactcgaac ggcagtcgtc
      841 gttccgcagt catgaggtgt ggtcgaagct ggaaattcat tgcgtgctct ctttctgacg
      901 agttcggacc attctcgagc tttgtcgatt ctgatcgtgc tcgcgtgctc tgctcggtgt
      961 gagaaggcgg gttgacattc cgggttgacg aattaaacta aaccgtatag tttatataaa
     1021 tcacgaacga gcaggcctgt caagcagaag ggctcacgcg acccgctcag gcttttggtg
     1081 agcgcggcaa gtccctgaaa tcccggcttc ttacgaaggc ctgcttcgga acgaaaccca
     1141 agatccatga gaggagtgat ctgacatgat ttcgaaaacc agacgtccct gtatcgcagt
     1201 ggtggcggca ttgctttgcg cgacaactgc ctctgcgcag gaaaatcacg gcacccccga
     1261 gcagagggcc gcgtgcgcgc ccgatgcctt caggctctgc gcgggctaca ttcccgaccc
     1321 caccagggtc gagcactgcc tgaggcagaa caagtccgat cttagcgacg cgtgccgatc
     1381 ggtgttcgaa caaagtgccg gcccggtggc cagcagagtc aaataacgga atcgggctgc
     1441 cccataggag ggcggccgaa ttctgcgcaa acgcggcaga gacgccgtgt gagccagtaa
     1501 caaggagata acgagatgga catgttctac attcccgcga ttgcgtcgtt ttcaggatcg
     1561 gcttctggaa cgcttgcgtc gattgtcacc ggctgggtga cccagcgccg caaggaccgc
     1621 atccgtcaca gcctgcgagc cacgtcccag cgcgaagagc tctacaagag cttcatcgag
     1681 gaagcatcga ggctgtacgc cgacgccctc gtcaacgata agtccgaggt atcgaaactc
     1741 gtcgacctct acgctctcat cgcgcggatg aaaattcttt cgagcgatgc cgtgatcgaa
     1801 gcggccgaaa aggcggggcg ggtgatcctc gaaacctatc tttcgccgaa caggacattc
     1861 gtcgatctgc ccgatctgat caacgagatg gatccgttgc gcgatttcag cgaggcttgc
     1921 cgccgcgaat tgcaatcggc tccgcagcgc tgaggctcct tgatcatgcc tttgctgcaa
     1981 tatttcggat gggtaggaag tcttcttctc gcggtgcttc ttgccgcgaa ctggtgcttc
     2041 cccgcaccgg ttgctccact gtccgacgtt tcgctcgatc ggaagatcga cattcgaatc
     2101 cataccgatc ataaatggcc tgagcgtgtg gtgttcgaca ccaccgatgc gatgccggcg
     2161 caaaaagcca gcggggaagc cggcatcggc ggaagcgagc cagccgttgt ggcggaacgc
     2221 cagcccttcg acgcgttcgc ccaaatggcg gccattcccg tcagaccgta ttttcgaccg
     2281 ccctgctccg ccggcccagc cgcagcgcgg gaggattcgc ggtttgggaa agccgcacta
     2341 tctcagtctc gcgcgcactt ggcggcgcgc aaggacctta ctttccccag tcggcttcac
     2401 aagccgccag gaaaaagctg acgcgctcgc gtacgaaagt gggcaactcg ctgcgcaggt
     2461 ctttcgcgcc ttcccccatc agagcccgca tcagcatcgg cagcagaatc agatccatga
     2521 aaatctgcgc cgtggtgagg cttcgtttcg ggccgaatgg tcccttggag ccgcgcgaaa
     2581 gtgtatgggt ggcatcgttc aagacgtggg acacggcagc cgcggcgcgg tcccgcccgg
     2641 cttcgtggac gtggcggctc aaggccggga atctgtcggc ctcggctatc gtggcgcgcg
     2701 tgacgccgat tgtctcctcg atgaatcgtt ccacgacctc gatgccgagg ctcgcaagct
     2761 tgtcctgcac cgcgcgtccc ttgggctcaa agccttcgaa attcgtcaat ccgtcgatga
     2821 cgcgggccac aaccgcctcg aacagcgcct ccttcccggg gaagtgggca tagatggtcg
     2881 gcttgctcac cggcgccgtc tcggcgatct cgtcgagact ggcgctttga taacctctct
     2941 tgaggaatac ccgctgggct gcatccagga tccgcgcttt cacgtcgccc gcaaggtcct
     3001 tgggcggtct tcccggacgc ggccgtttca aatcattgcg ctttgtcatc atctccaccc
     3061 ttgcattcac gatgctgcga gcgcccaagc tcgctgacaa ttccggcttg acacgtgttc
     3121 gatcagggcg caagtaaaat aaactatacg gtatagttta aatcaatgga gaccccccct
     3181 gtgtccgatt ccccacccta tccgcccgcc ctggagggac gaaccctttt ggacgagctg
     3241 aatcatcgca tcaagaacga actcgcctcg gtcatcaatc ttgtcacctt caaggccgtg
     3301 tggaccgaca atgccgacgc caaggaagag ttgagcaatg tcgtcgactt gcttcaccag
     3361 catgtcgagg tccacagcat tctgacaatg ccggatcgcg acgcgcttgt ggacgccgcc
     3421 gaacagcttc gcaagctggg actggcgatg agccgttgca agctggatcg catgaacatc
     3481 cgcctcatgt tgtcggctga cgcgctgccg ctggagtcgg agcggtgctg gcgcctggca
     3541 ttggccatct acgaactggt gaacgaacgc ggtccgccac gcttgcttcg atggccggga
     3601 cggtgaaatc aagatcaggc tgatgcgtgc cggctcgtgg gtgaactgcc acgtcagcga
     3661 caacggatcg ggcgcgggaa ggatcaagct cggtcgaggc ctgcgcattg tcggcgatct
     3721 ggtcgcgagc ctgggcggcc ggatcgaccg cacctccggc acgacctgga actcgttcgc
     3781 cctggacttt cctctgacgc aacgcgagca gcgcgccaac cgcgtggtcg tcgcccgccg
     3841 ccccagaacc ggtcgtcggc caaggacgat gccgtcggta gcctccgtgt cagccgcaag
     3901 cgaaccggca aatccgatcg ccgttcagcc acaactctaa gatcgaagga gatatacaat
     3961 gtcgacaagg actcttctca ccctgacggc gagcctggtg ctcggcgccg ccatcgccgc
     4021 tcccagcgcg gcactcgcgc agttcggccc tcctccggga cctccgcctg ccttcgctgg
     4081 tccgcctccc ggcctcggtg ccggtggtcc tcctccgggt ctaggcgccg gcggtgctcc
     4141 cctcggtggc gttccggccg gacctgtcgc tggctttccg ccccgcggcc ccgcgggcgc
     4201 acctccgcgc gacatcgccg gtggtccgcg tctcgagggt gctggcggat tgcgtggcct
     4261 cgaccgcggc ggccaggcca attttcgcgg cgtcgaaggt cacgccgcgg cctacagcgc
     4321 caacagctac acccgcaata gctacaacag ctatggccgt ggctatggct acggccatgg
     4381 ctcccgctac tggccttatg cggccgcggc ggcgggtgct tacgcctatg gcagttccta
     4441 cacgtcctcc gacgaaggct gctactacgt ctccacctac aggcgatacg gcaccaggcg
     4501 cgtcctggtg tgtcatggag atggagactg attgagacgg acggcccttt ggcgccccgg
     4561 tccccgcacc ggggcgcccg tcgtttggcc gaacgcaatc tgcaccgtca gagccgtttt
     4621 tcgggcttga caacctcgta aacaaactat acggtttagt ttaattatag caggagagcg
     4681 ccgtgcgcca ttttgtcccc cgtccccccg cgtcggccat ttcgaggtcc atgcgtgccg
     4741 cactcgcgct cgtgctgctg gcggcgccgc tgagtgcctg caatgacccc gccaccaccg
     4801 tcgagcgcgc cgcgttcgtg cgcaccgaga tcgtgcggcc gcgggacgga caggcgtccc
     4861 tcacgctgac cggcgaagtc caggcccgct tcagcgccga cctctccttt cgcgtgaacg
     4921 ggcgcgtgct cgcgcgcctg gttgatgtcg gcgcccacgt caatgtcggc gacgtcctgg
     4981 cacggctcga tccggccgag caacaggccg atcttgatgc tgcgaccgca ggggttgccg
     5041 ccgcggagtc ccagttgcgc gtggccaaag ccacgtttga tcggcagacc tccctcatct
     5101 cctccggctt cactacccgc gtggcgtatg atcaggcgca ggagcaattg cgaagcgcag
     5161 aaagtacgct tgaaaccgcc aaatcccagt tggggcaggc ccgcgaggcg cttggcgaca
     5221 ccgaactgca cgcccgtgct tcgggtgtga ttaccgcgcg aaaccttgag gtcgggcaag
     5281 ttgtgcagtc ggctcaatcg gttttcacgc tggcgcagga cggtgaccgc gacgcggtct
     5341 tcgatgtccc agagtcgttt ttctctggcg acgtggagag caggcgcgtg tcgctgacgc
     5401 tggtctcgga ccctgacgtg accgccgtcg gtgatgtcag ggaattatcg cctgctgttg
     5461 atcccaagag cttaacggtt cgggtgaaag tcgcgatcca aaatccgccc gccgcgatga
     5521 cgcttggcag cgccataacg ggcaccgccg gcacgaaagc cgccagggag atcgccttgc
     5581 cttggaccgc gctgatggca acgggttcaa agcccgcagt ctggatcgtc gacccccgga
     5641 cgaagacagc ctctctcaag ccagtgtctg tcgccggcta tgaagcccga gcggtgctga
     5701 tcaagggtgg cctcgaggct ggagaccgcg tcgttgtcga tggcggcaag ctgctcagct
     5761 ccggccagcc cgtgacgtat gatgaggatc gatcatgatg acgcgcgctc ttgcccttgg
     5821 cagcatcttc gctttggcgc tggttctgac gggatgcaaa caggagacaa agggcgagga
     5881 gcccgtccgc cctgtgctgt cccatgtcct gaagccgacc gcgccgggca atactcaggc
     5941 cgtcggaact gtgcagcccc gatacgagac gaacctcggc ttccgcgtgc tggggcggct
     6001 catcgcacgt ccagtcaacg ttggagatct ggttgccgag gggcagacta ttgccgcaat
     6061 cgacccgact gtcctcgagc tcgccgtacg gtctgcgaaa gccgaccttt cgaaagccca
     6121 ggctgtgctt gaaaacgcca gcgcaagtga ggagcggaaa cgaatactga tcaagaccga
     6181 tgccacgacg aaacagaccc ttgatgatgc tgagcagatg cgcgcgggcg cacaagcatc
     6241 gaccgcccgc gcgcaggcga acctgacgaa ggcaatcgag caactcggtt atgcacaggt
     6301 caaggccgat ttcgccggcg tcgtcacagc cgtaggcgcc gaagtgggac aagttgtttc
     6361 gcccggtcag cgtgtcgtga cagtggcgcg gccggaaatc cgggaggccg tggtcgatat
     6421 cggcgaagac tttccggtgc cattgaagat cgatttgccg ttcgctgtca gtttgcagct
     6481 gcggccggcg cttcaagtgc aaggccagat tcgcgaaata gcgccgcaag ccgattcggt
     6541 aacgcgcatg cgccgtgtcc gcatctcttt gacggatccg cctgagagct ttcgccttgg
     6601 atccaccgtt acggcgagac cgaacaacag cgagaactcc gttctccggg tgcccacgtc
     6661 cgccgtcctt accagggata gtcagaattt tgtttgggtg gtcgatcccc tcacgggcac
     6721 ggtttccctg cacaaggtcg atctcgcgga ggacgaaggc ggcctgcggg tcacggacgg
     6781 gctcccggaa ggcgcgcgga tcgtcaccgc cggcatccat agcctcaaac caggacagca
     6841 agttcgtttc gaacaggatt tggcaccatg atatcgttca acctttccaa ttgggctctc
     6901 ggacatcgtt cgctcgtctg gtatttcatg ctcgccttca tggcggcggg actgttctcc
     6961 tatctgcagc tcgggcgcca ggaagacccg gatttcacca tcaagaccat ggtgatccag
     7021 gcgcaatggc cgggcgcatc tcctgaagag atgacgcgcc aggtcaccga ccggatcgag
     7081 aagaagctcg aggagctgga atcgctcgac tacaccaaga gcgtcacggt tgccggccag
     7141 accacggtat acgtctatct gcgggacacc acgaaggcgg ctgacgtcaa gccgacctgg
     7201 gttcgcgtcc gcaacatgat cgcggacatc aagggcgatt tcccgcaagg cgtgatcggg
     7261 ccgggcttca acgaccgctt cggcgacgtc ttcggcaaca tctacgcctt caccagcgac
     7321 ggcctgaccc agcgccagct gcgcgatgag gtcgaggaca tccgcgccaa ggtcttgacc
     7381 gtgccggatg tgggcaaggt cgacatcctt ggcgcgcagg acgaggtgat ctacctcgaa
     7441 ttctccaccc ggaagatcgc ggcccttggc ctcgacatcc actcgatcat ggcctccctg
     7501 cagggacaaa acgcggtcgc gcggtccggg gtgttccagg aggggcccga gcggatcagc
     7561 gtgcgggtga acggccagtt tacgtcggag gcaagcctga aggcggtgaa cctacgcatc
     7621 aacgaccgct tcttcccgtt gactgacgtc gcaaccatca cgcgcggtta taccgatccg
     7681 gccaagacgt tgttcagata caacggcgag cccgcgatcg cgcttgccat cggcatgaag
     7741 tccggcgcaa acctgcttca cttcggcgaa gcgctgaagc aggaaatgtc gaaggtcatc
     7801 gccgacctgc cgatcggcgt cggcgttcac ctggtcgccg accagccggt catcgtcgag
     7861 catgccgtgt cgggcttcac cgaagcgctg ttcgaggcgg tggtcatcgt tctcgccatc
     7921 agcttcatca gccttggcat gcgggccggg ctagtggtcg cgattgcgat tccgctcgtg
     7981 ctcgccatca catttgtcgt gatggcctat gccgacattt cgctgcagag aatttcgctt
     8041 ggcgcgttga tcatcgcgct aggcctcctg gttgacgatg ccatgattgc cgtcgaaatg
     8101 atggtggcca gactggaggt cggcgatccc ctggagaagg cggcgacaca cgtctatacg
     8161 tcaactgcct ttcccatgct gacgggaaca ctggtcaccg tcgccggctt catcccgatc
     8221 ggcctcaaca gcagcaatgc cggcgagttc accttcaccc tgttcgtggt gattgcggtt
     8281 tccctgatcg tgtcgtggat cgtcgcggtg ctgttcacgc cgctgctcgg cgtcgccatc
     8341 ctgcccgcca aaatgaaggg tcatcatggg gagaagggcc gggtggcgca aatgttcgcc
     8401 cgcttgctgc tcgtctgtat gcatcaccgc tggacgacgg tcggtgtgac tgttgcggcc
     8461 ttcctcatcg ccttatttgg catgaagttc ataccgcagc agttctttcc ctcctcggac
     8521 cgcagcgagt tggtgatcga ctggagtttg ccgcagaacg cctcgattgc cgacaccaac
     8581 gcgcagatgg cgcgctttga acgggaaaag ctgcagggca atggttcagt cgagcactgg
     8641 tcgacttatg tcggcaccgg cgctccgcgg ttcgtcctgt cattcgatgt gcagacggct
     8701 aatacctggt tcggccagca ggtcgtcgtg actaagggcg gcatcaaggc gcgcgaccag
     8761 gtcaaggcgc agttcgagga ctatctgaag aagacgtttc ccggcaccga tacctatgtc
     8821 aagttgctcg aggtcggtcc accggtggga cgtcccgtgc agtaccgcat gagcgggcca
     8881 gacatcgcga aggtcaaaga tctctcgcag cagctcgccg gcatcgtccg aagcagcccc
     8941 gatctcggca acgttgtgtt cgactggatg gaacctgcgc gtgtcgtcaa ggtcgatgtt
     9001 ctccaggaca aggcgcgcca gcttggtgtg acctcggaag acatcgccac ggcattgaac
     9061 tccgtgctcg aaggcacccc gatcacccag gttcgcgaca gcatctacct cgtgaatgtg
     9121 acgggacgcg ctactgcggc cgagcgtgcc tcgatcgata cgctgcgcga cctgcaattg
     9181 cccggggtcg gcggtcagtc ggtgccgctc ggagccctgg ccaatctacg ctatgaactc
     9241 gaacagccga cgatctggcg gcgcgcgcgg attcccacca tcacgctcaa ggcggccacc
     9301 gtcggcaacg ctcagccgaa aaccgtcgtg gatcaactcg cgccgaaggt ggcggagttc
     9361 gccaaggaac tcccggcggg atattcggtg aagatcggtg gctccgtgga ggagagcgcc
     9421 aagagccagg ctccgatcgt ggccgtcgtt ccgctgatgc tgttcgtcat ggctaccgtc
     9481 ctgatgatcc agctgcaaag cttccatcgt ctgttcctgg tgttcgcggt cgcgccgctc
     9541 gccgtgatcg gcgtcgtcat ggccatgctg ccgagcggcg cgccgcttgg gttcgtcgcc
     9601 attctcggcg tactggcgct gatcggcatc ctggtccgaa actcggtgat cctgatcgtg
     9661 cagatcgagg atctgaagaa ggagggacgc ggagactggg acgccgtggt ggaggcgact
     9721 gagcatcgca tgcggccgat cctcctgacc gcggccgccg caagtctggc gctgattccg
     9781 attgcacgcg agattttctg gggcccgatg gcctacgcga tgatgggcgg catcatcgtg
     9841 ggcacgctgc tgacgctgtt attcctgccg gcgctatacg tggcgtggtt tcgcatcaag
     9901 gagccggagc ctgcgatgaa ggacattcaa aaaaatccga ttccgtggga atcgcgaaca
     9961 gatctgccgg ctgatgcgcc acatcggcac gaaatggaaa atgcatagga gcgaagtatg
    10021 agagaagttt tgagtctcgc cacgtttgcc ctcggggtgt tatgctttgt tgcgttcgtg
    10081 gccgcggtaa agccgaatgc gtttgcggca gccgaggcgc cgaacaagcc tgcccattgc
    10141 caaacggatg gctcgcaggc gcgtggatgc ggtttgcacg tctttgccgc agcatcaggc
    10201 ttcgggcagc ggtggtgaca gtcagagggt cgacgtccgc ttagacccgc gtgcgctccc
    10261 tcaccccgat tgaaattctt gattcgatct cgtaatcatc cgcgcggatt cgcttgattc
    10321 agcgattcgg ttttatccat tagggtaagc atatgaccac tctcgttgag accccaaccc
    10381 agattgcggc ccgtgcaggc acacagtatt tttatttcta catggcgctg tcgtgcacgg
    10441 ccgtggcgtt ttttggcttc gccccgacat actggctgcc gatggcggca cgaacattca
    10501 agtcgagccc gatcgtccac attcacgggc tggtattctt ctgctggacc ttgttctttt
    10561 gcttccagac ctggctcgcc gcgtcgggaa ggatcgcgaa ccaccgttcg atcggcatga
    10621 tcggaatatc ttttgcgacc gcgatgacca tcttcggcgt gctggcaagc atcaaccaga
    10681 tgaagaccgc ggcggcggtg ggactgaaag acgaaggcat tgcctttgcg atcgtcccgc
    10741 tgagcggcat cctgttcttt gccgtcgcgt ttagcttggc catcgcagcg gtgcgacggc
    10801 ccgcgcagca caagcggttg atgctgcttg cggcgatctc ccttctcgac gcggcggtag
    10861 ctcgctggtt ccttaccttt ctggcgccgc ccgggccccc tggaccgccg ccggtcgcgg
    10921 tcaccctgct tccggcgttg gtcgcctatc tgctgctggc ggcggccatc gttttcgatt
    10981 ggcgcacgcg tggacggccg catccggtct atgtgttcgg cggggcggcg ctggttgcga
    11041 tcaaggtgct gaacctgccg atcagcgcca cctcgtggtg gcattccttt gccggcgaaa
    11101 tattggcgct cgcgcaatag cgcaaacggc ttgaggccgg ggtgcttgcg ggctgcgccc
    11161 cggccgattt aagcttgcgg accctccaac tggaacagga gggtgaaggt gacccacctt
    11221 gacccccgag gtccgaatgg actggttcgg ttggcagctc cccaaggatc ccgcggcgga
    11281 caaaagcgcc gccttcggtc cgcaagccat tcttcaggaa ctgggcaagc gcgtcccgca
    11341 atacctcgat gacatcgatc aaggcaggct gatctatccg gcctgcaaga gaacgccttc
    11401 ggatccccac ggcgacatcc gctcgatctg ggaccatacc cggctggaag cagcccgtta
    11461 tgtgagcatg gtaccgcggc gcgagttcga accgttggcc gagcccgcgc gtcagcccga
    11521 cgtgctggag gcctatcttc gccagcggcc acatgaagac accgtgattg agttcaccgg
    11581 ctccacgatg ggcgatctgg cgattgccat catcgccggc ttcaactggc tgaaccactg
    11641 tgccgctctg gcgggatccg atcgtgacaa attctcaggc acactcagca atttcaggaa
    11701 ggtcgcgggc ctggcgtggc aatggtgggc catcgagggc gccggtgagc gctgcagcca
    11761 gatgctggcg gaaggcgaga aaccgccgct gatgctcaat ttgctctgga cggaatatac
    11821 ccggctggca aaggaggtgg cggccgcggc attcttcgga tcgtccatcg agcaggcgat
    11881 agccgggcga ggcgaagttc tcaaatcgga attcgccggc cgccccgagg agctcgactc
    11941 ggccctcggc gaacttgcgg aaaccatggc cagtttcgag cgcgcccgcg atcccgatga
    12001 tctgatcggc taaagcattt tccccttttc cagtcatggc cgggcaaaag cgcgaagcgc
    12061 gtctttgcgc tagatgtccc ggccatccac gtctttcttg ttttaacttg ttttaagtcc
    12121 ggcaaagacg tggatgcccg gcacaaggcc gggcatgacg agcttcattc attggctgct
    12181 tttgagtcag gctctcggga tgaagtctta gtctagcctc cccgagccgg ttgagccgcc
    12241 aacaacgtct cgaactcggc aggggcaaag actttcccct cgtcgttggt aatggtgact
    12301 tgccaacctt cgctggccca cacccgcgcc cttgccatcg tgatcagtga gctggtcctt
    12361 tcgcttttca ccgtctcgtt tccgcgttct gcgatcattt tgtagaccaa ccctcattct
    12421 ccttggtgtg ttcccagcat tgacgtcatc ttgatcgatg gttttgactg caatctgccg
    12481 acaagatgac gatttggcgg cgcaagctcg agttgcgttg gcctcgctca tgtcaggctc
    12541 gaccctgaaa tgaacacggc tagttgatca ggtaggcgtc aacgaccctt ggacataacg
    12601 gattcgtaac tagttcgtgg actggtcgag gcaaagaaag aatgctcaga gagccgcatg
    12661 cggaaaatcg gcgattcaag cgctttgcgc gccattttac tcgcatgggc attatctctg
    12721 acctgcctgc ctacgaatct catggctgcg ccatcggatt cagtgcaaac ggtcgatcag
    12781 gccggtccat ccgatgagcc attcggactt tccaccgaaa ccgtgaccag gggcacggcg
    12841 ttggagaaat ggctcaacgt cgagcaccaa attgacaatg atcggctggt gctgaagacg
    12901 tgccaagaga accgcgcatc ctgccaatcg ccggccgctc ttcaatttct ggccattgtc
    12961 gacattgggc gaaacctgga gggtcgcgcg cgcctcggcg agatcaaccg cgccatcaat
    13021 ctgaaactaa agcccaagag cgatctggca ctttacggtg aggaggatgt ttggacctcg
    13081 ccgctggcca ccttcgccat cggtgccggt gactgcgagg attacgcgat tgcaaagttt
    13141 gttgccctgc aggaggccgg ggtttccccc gacgaccttc gcattgtcat cctgcgcgac
    13201 gaccaccgcg aagaggacca cgccgtcgtc gcggccaggc tcgacggaaa ttggctgatg
    13261 ctggacaatc gccacatggt cctggtcgaa gaccagctcg tgcggcgtta tcgcccggta
    13321 tttctagccg atcgtgacgg cgtcaagctc tactccgaca cgccagctct tgccgggggc
    13381 tcgcgcggat atgagcgggc catcggtcaa atccggcgct agtgttgtga ctctaacgct
    13441 tgtttcgccc gggcgacttt ttcgaggatt tggcctgcgg actttgtcca gacgaagagt
    13501 ttgggatcgg cattgtggtt ctccaggtat tgcatgatgg cgctcttgag ttcg
//
LOCUS       DATNOI010000288         9168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2192474,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000288 DATNOI010000000
VERSION     DATNOI010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9168
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192474"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VN968_11235"
     CDS             complement(<1..519)
                     /locus_tag="VN968_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXL29849.1"
                     /translation="MRSASGGPRVLLRRLRETMAEQVSAQERLDKIVVLIAANMVAEV
                     CSTYVLRVDNTLELYATEGLNRDAVHRTVLSAHEGLVGLVASEATPLNLSDAQSHPAF
                     SFRPETGEEIYHSFLGVPILRAGNTLGVLVVQNRAKRTYVEEEVEALQTTAMVLAEMI
                     ASGELAALAQPGA"
     gene            complement(785..2038)
                     /locus_tag="VN968_11240"
     CDS             complement(785..2038)
                     /locus_tag="VN968_11240"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HXL29850.1"
                     /translation="MGRLVMKFGGTSVASIDRIRNVAQHVKREVDAGHDVAVVVSAMA
                     GKTNELVEWCREASPMHDAREYDAVVASGEQVTAGLLAIILQGLGIQARSWQGWQIPI
                     KTSDAHASARILEIDGSELINRFKERKEVAVIAGFQGIYPATGRITTLGRGGSDTSAV
                     AIAAAIHATRCDIYTDVDGVYTTDPRVVPKARRLDKIAFEDMLELASQGAKVLQVRSV
                     ELGMVHGMPVFVRSSFDRPEDIDPHATPPGTLICSEEEIMENHVVTGIAFSKDEAQIS
                     VRQIQDKPGVAAAIFGPLADANINVDMIVQNVSEDGATTDLTFTVPAADYARALDTIT
                     GAKSRIGYARLDSATDVAKVSVIGSGMRSHAGIAAKAFAALAARNINIRAITTSEIKF
                     SVLIDAAYTELAVRTLHTLYGLDQV"
     gene            2210..2677
                     /locus_tag="VN968_11245"
     CDS             2210..2677
                     /locus_tag="VN968_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXL29851.1"
                     /translation="MGRYIDDILQPGEKVLYSTNAHWIFYWPAIAAWIVALFLFLLSR
                     QTTTESIVLVCLAASAVVAIVALYWTVRAWFHRWTTETDVTNMRVVHKTGFIKRRTFE
                     MSLDKVESVDVNQSILGRIFNYGDVTVRGVGEGAETIDTIASPLEFRNHITAR"
     gene            2694..3461
                     /gene="ubiG"
                     /locus_tag="VN968_11250"
     CDS             2694..3461
                     /gene="ubiG"
                     /locus_tag="VN968_11250"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HXL29852.1"
                     /translation="MAMQENSRSNFPAPASTVDPGEVAKFSRLSDEWWDPNGKMAPLH
                     KINPLRLSYIRDAACRNFERNSKSLNCLSGLRILDIGCGAGLLCEPLTRLGAQVIGID
                     PSASNIAAARLHADKGHLSIDYRCTSVEEMDVRERFDIVLAMEVIEHVTDVGTFLNRC
                     AAMLKPGGLMLVSTINRTWKSFALAIVGAEYILRWLPRGTHQWDKFVTPDELARHLAN
                     NKLAITEQAGVIYHPFADRWSLSSDMDVNYMVVAEGV"
     gene            3611..4510
                     /locus_tag="VN968_11255"
     CDS             3611..4510
                     /locus_tag="VN968_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXL29853.1"
                     /translation="MFSIATLWIPFTITAALAQVARNAMQRQLTGPLGTWGATNIRFL
                     FGFPFSVVFFAIVLAATGDRVPWPTAGFWPWLLLGALSQILATGLMLVAMNDRSFVVT
                     TAYLKTEAIQTAIFGFVFLGDHLTALKVIAILIATVGVVITALRPGGEKGFAELKPTI
                     IGLVAAAAFALSAIGFRGAIITVSGVSFVTAASYTLVFGLFVQTLVLTIYLLARAPEV
                     LAKILGLWKPSMLAGFMGAFASQFWFLAFALTAAANVRTLALVEVLFAQAVAYYSFKQ
                     PLSGREFLGIVLIVIGVALLVAV"
     gene            complement(4632..5120)
                     /locus_tag="VN968_11260"
     CDS             complement(4632..5120)
                     /locus_tag="VN968_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HXL29854.1"
                     /translation="MIRYSLRCEAGHEFESWFQSSSAYEQQEKRKLVNCPACGSAKVE
                     RAIMAPQIVSRKGREIAAPEPAAAPAPAPEATTAAATSTPLLMAQERELRAKLKELRD
                     HIVKNADNVGERFPNEARKMHYGDIEHRPIYGEASPDEARALIEEGVEVSPLPVLPED
                     RN"
     gene            complement(5117..5998)
                     /locus_tag="VN968_11265"
     CDS             complement(5117..5998)
                     /locus_tag="VN968_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HXL29855.1"
                     /translation="MSAELSFNAAMVQMCTGLLPGPSLEQGTRLIRQAAAQGADYVLT
                     PEVTNMIQSNRKALFENLAPEEDDLSLKAYRALAAELKIHLHIGSLALRASPERAVNR
                     SFLIGPDGGVIASYDKIHMFDVDLPGGESYRESANYQPGEAAVLADLPWGRIGLTICY
                     DMRFPALYRALAESGASFLTVPSAFTVKTGEAHWQTLLRARAIETGCFVFAAAQAGTH
                     ESKRQSYGHSLIVDPWGTVLAEGGGVETGVFMATIDPAKVETARKAVPSLQHGRRFSV
                     ADPKAGPDHLHLVRGSA"
     gene            complement(5995..6270)
                     /gene="grxC"
                     /locus_tag="VN968_11270"
     CDS             complement(5995..6270)
                     /gene="grxC"
                     /locus_tag="VN968_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HXL29856.1"
                     /translation="MTAAIEIYTRPGCGYCGAAKSLLTRKKAAFIEFDVARDPARRQE
                     MWDRAGAGSTFPQIFVGEMHVGGCEELYALDRQGKLDALLAGEKANS"
     gene            complement(6301..6645)
                     /locus_tag="VN968_11275"
                     /pseudo
     CDS             complement(6301..6645)
                     /locus_tag="VN968_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
     gene            6802..8079
                     /locus_tag="VN968_11280"
     CDS             6802..8079
                     /locus_tag="VN968_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HXL29857.1"
                     /translation="MPEKLLKKNEIDGLLKKPGKHRDGAGLYLQIARRGQASWVYQFR
                     WNAETKWMSIGPAATFKLEEARKRHHELRRMRDRGIDPRSSVAAVALQPYQPSPSMAP
                     AAPDAPEPPEQPASPLFGEVVELFLNDFAADWAGGFEGKEAKSYRRTLITKSSLAKFA
                     VAEIRTEHVEADLKQWADKPVTAEKVLMRIGKILNYASAKNFRSKEVPNPARIKGHFE
                     FLARPVVPETVNHPAMKIPDVPGFMRELIADGSIEARALAVLILTGPRTDELRLARWK
                     EIDGTIWTIPAERMKGKKKDRRAHSVPLVPAVVKLLGKRGADNDFIFPSRRYGARKPL
                     WHSSLREVLNRLRGDRLSIEGLPPVPHGFRSTFRDWAAEAGYPRDLAERAIAHKVGGK
                     TETAYQRSKLIEQRRPLMVAWAKFCISGGRRND"
     gene            8072..8251
                     /locus_tag="VN968_11285"
     CDS             8072..8251
                     /locus_tag="VN968_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29858.1"
                     /translation="MTDIREAERLALITHAQVVLVLEEAENAELEARAFSLDRARQRR
                     PSGPHVCSVIPFPKR"
     gene            8602..8898
                     /locus_tag="VN968_11290"
     CDS             8602..8898
                     /locus_tag="VN968_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29859.1"
                     /translation="MDATLEPTSSSVDVANKRITTPLGEYRISRVEDAQLYFDKPASP
                     TFNFVTHGTLDRTTGKMLIRWMRPEEDAKVQVVGQTAHMARYAEFTCSTAKRLF"
BASE COUNT         1703 a         2800 c         2854 g         1811 t
ORIGIN      
        1 ggcgccgggc tgcgccagcg cggccagttc gcccgaggcg atcatctccg ccagcaccat
       61 cgcggtggtc tgcagcgcct cgacctcttc ctcgacataa gtccgcttgg cgcggttctg
      121 caccaccagc acgccgagcg tgttgccggc gcgcaggatc ggaacgccga ggaatgaatg
      181 atagatttct tcgccggtct ccgggcggaa cgagaacgcc ggatggcttt gcgcgtcgct
      241 gagattgagc ggggtcgcct cgctcgccac caggccgacc aggccctcat gcgcgctcag
      301 caccgtcctg tgcacggcgt cgcggttaag gccttcggtg gcgtagagct cgagcgtgtt
      361 gtcgacgcgc agcacatagg tcgagcagac ctcggccacc atgttggccg cgatcagcac
      421 cacgatcttg tccaatcgtt cctgggctga gacctgctcc gccatggttt cgcggagccg
      481 tctcaataag acgcggggac ctcccgatgc gctccgcatg tactaaaatc ctcctccgcc
      541 aagccagcct gccgggtggc cggcagctgg cattaagctc cagaaaaaca acaattttat
      601 tcattcggcg ccgccgcaag ccgggatatc ggccgaatca ggatagccaa actgtgacac
      661 agtttgcggc acccatccta aaggccgtat agcgaattga ggcttgtttt gccaagcaaa
      721 acgcctgcca gaagcgataa cgtctctggc actccaatgc tgcgctagct aagggaaaat
      781 tcttctaaac ttggtcgagc ccgtagagcg tgtgcagcgt gcgtaccgcg agttcggtat
      841 aggccgcgtc gatcagcacc gaaaacttga tttccgaggt ggtgatggcg cggatgttga
      901 tgttgcgcgc ggcaagggcg gcaaacgctt tcgcggcgat accggcatgg ctgcgcatgc
      961 cgctgccgat cacggagacc ttcgcgacat cggtggcgct gtcgagccgc gcataaccga
     1021 ttctgctctt ggcgccggtg atggtatcga gggcgcgggc ataatccgcg gccgggacgg
     1081 tgaaggtgag atcggtggtg gcgccgtcct cggagacgtt ctgcacgatc atgtcgacgt
     1141 tgatattggc gtccgcaagc ggaccgaaga tcgccgcggc gacgcccggc ttgtcctgga
     1201 tctggcgcac ggaaatctgg gcttcgtcct tcgaaaaagc gatgccggtg acgacgtggt
     1261 tttccatgat ttcctcctcg ctgcagatca gcgtgcccgg cggtgtggcg tgggggtcga
     1321 tatcctcagg cctgtcgaag ctggaacgca cgaataccgg catgccgtgc accatgccga
     1381 gttccaccga gcgcacctgc agcaccttgg cgccctggga ggccagttca agcatgtcct
     1441 cgaacgcgat cttgtcgagc cggcgcgcct tcggaaccac gcgcggatcg gtggtgtaga
     1501 cgccgtcgac atcggtatag atatcgcagc gggtcgcatg gatggcggcg gcgatcgcga
     1561 ccgccgaagt gtcggaaccg ccgcgcccga gcgtggtgat tcgtccggtc gccggataga
     1621 tgccctggaa gccggcgatg acggctactt ccttgcgctc cttgaaacgg ttgatcagtt
     1681 ccgagccgtc gatctcgaga atccgagcgg aagcgtgggc atcgctggtc ttgatcggga
     1741 tctgccagcc ttgccaggag cgcgcctgaa tcccgagccc ctgcaggata atcgcaagca
     1801 gcccggcggt gacctgctcg ccggacgcca ccacggcgtc atattcgcgc gcgtcatgca
     1861 tcggcgacgc ctcccggcac cattccacca gttcattggt tttgccggcc atcgccgaca
     1921 ccaccaccgc gacgtcgtgg ccggcgtcga cctcgcgctt gacatgctgc gcgacattgc
     1981 ggatgcggtc gatgctggcg accgatgtgc cgccgaattt catcacgagg cggcccatga
     2041 tgctggtgca ttccctgtca gggtgagtaa tgtgatcggc acgcaaaacg gcaagcgcgc
     2101 aaaccaaaag gcgcgtatac atagcggcgc ggtttccggc aagcaaccgg gttccggcga
     2161 ttccgggcgt tacgggccgt ggtgatgttg ggtaattgag gctgaatgca tggggcgtta
     2221 tatcgacgat atcctgcagc ccggcgaaaa ggtactgtat tcgaccaacg cgcactggat
     2281 cttctattgg ccggcgattg cggcctggat cgtggccctc ttcctcttcc tgctgtcgcg
     2341 gcagaccacc accgaaagca tcgtcttggt gtgcctggcg gcttcggccg tggtggcgat
     2401 cgtggcgctg tactggaccg tgagggcctg gttccatcgc tggaccaccg agaccgacgt
     2461 caccaatatg cgcgttgtcc acaagaccgg cttcatcaag cgccgcacct tcgagatgag
     2521 ccttgacaag gtcgagagcg tcgacgtcaa ccagagcatt cttgggcgca ttttcaatta
     2581 cggcgatgtc acggtgcggg gcgtcggcga gggggccgag accatcgata ccatcgcttc
     2641 cccactcgag ttccgcaatc acatcaccgc gcgataggag cggcgcgcaa tccatggcca
     2701 tgcaagaaaa ttctcgcagc aatttccccg ccccggcgtc cacggtcgac cccggcgagg
     2761 tcgcgaaatt ctcaagactg tcggacgaat ggtgggatcc caacggcaag atggcgccgc
     2821 tgcacaagat caacccgctg cgcctgagct atatccgcga cgccgcctgc cgcaacttcg
     2881 agcgcaatag caagagcctg aattgccttt ccggcctgcg catcctcgat atcggctgcg
     2941 gcgccgggct gctatgcgag ccgttgaccc ggctcggcgc gcaggtgatc ggcatcgatc
     3001 cctccgccag caatatcgcg gccgcccggc tgcacgccga caaggggcac ctgtcgatcg
     3061 attatcgctg caccagcgtc gaagagatgg atgtgcgcga acgcttcgac atcgtgctgg
     3121 cgatggaggt gatcgagcac gtcaccgacg tcggcacctt tctcaatcgc tgtgccgcga
     3181 tgctcaagcc cggcgggctg atgctggtct cgaccatcaa ccgcacctgg aagagttttg
     3241 cgctggcgat cgtcggcgcc gaatacatcc tgcgctggct gccgcgcggc acccatcaat
     3301 gggacaagtt cgtcaccccc gacgaactgg cgcgccatct ggcgaacaac aagctcgcca
     3361 tcaccgaaca ggccggcgtg atctatcatc ccttcgccga tcgctggagc ctctcctccg
     3421 acatggacgt gaactacatg gtggtggccg agggggtgta gctattgtcg tccctgcgaa
     3481 agcattgcga aatctgggac gacatcgcgg ttggtgccgc atgcaatcct gacggttgac
     3541 cgcgcgggct gtgacattca cggtgctgac gattgtcagc gtcccgtgtt agccctccag
     3601 cccgcttccc atgttctcca tcgccacgct ctggattccc ttcaccatca ccgcggcgct
     3661 cgcgcaggtc gcgcgcaatg cgatgcagcg gcaattgacc gggccgctcg gcacctgggg
     3721 cgccaccaat atccgctttc tgttcggctt cccgttctcg gttgtgttct tcgccatcgt
     3781 gctggctgcg accggagacc gtgtgccatg gccgacggcc gggttctggc cgtggctgtt
     3841 gctgggcgcg ctcagccaga tcctcgccac cgggctgatg ctggtcgcca tgaacgaccg
     3901 ctcgttcgtg gtgacgacgg cctatctgaa gaccgaggcg atccagaccg cgatcttcgg
     3961 gtttgtcttt ctcggcgatc atctcaccgc gttgaaggtg atcgcgattc tgatcgcaac
     4021 cgtcggcgtg gtcatcaccg cgctgcggcc gggcggggaa aagggctttg ccgagttgaa
     4081 gccaacgatc atcggcctgg tcgccgccgc cgcgttcgcg ctgtcggcga tcggctttcg
     4141 cggcgcgatc atcaccgtat cgggcgtgtc attcgtgacg gcggcatcct acacgctggt
     4201 gttcggactg ttcgtgcaga ccctggtgct gacgatctat ctcttggcgc gcgcgcccga
     4261 ggtgcttgcc aaaatcctcg ggctgtggaa gccgtcgatg ctggcgggct tcatgggcgc
     4321 cttcgcctcg caattctggt ttttggcatt tgcgctgacc gcggccgcca atgtgcgcac
     4381 gctggcattg gtcgaggtgc tgttcgcgca ggctgttgcg tattactcgt tcaagcagcc
     4441 gctatcgggc cgcgaattcc ttggcatcgt gctgatcgtg atcggcgtgg cgctattggt
     4501 ggcggtgtag cagccctcat cctgaggagc gacctcttgg tcgcgtctcg aaggatgagt
     4561 gtcgacgtcc tcatggttcg agacgcgcga agtcgcgctc ctcaccatga ggaagactgc
     4621 gtatgtcgaa attaattccg gtcttccggc agcaccggca gcggcgatac ttcgacgcct
     4681 tcctcgatca gggcgcgcgc ttcgtccggc gaagcctcgc cgtagatcgg gcgatgctcg
     4741 atatcgccgt aatgcatctt gcgggcctcg ttgggaaaac gctcgccgac attgtcggcg
     4801 ttcttcacga tatgatcgcg caattccttg agcttggcgc gcagctcgcg ctcctgcgcc
     4861 atcaacagcg gcgtggaggt ggccgcggcg gtcgttgcct cgggtgcggg ggcaggggcg
     4921 gcggcaggct ccggcgccgc aatctcgcgg cccttcctgc tcacgatctg cggggccatg
     4981 atcgcgcgct cgaccttggc cgaaccgcag gccggacagt tcaccagctt gcgcttttcc
     5041 tgttgttcgt aggccgagga agattgaaac cagctttcga attcatggcc ggcctcgcag
     5101 cggagcgaat agcggatcat gccgatcccc gcaccagatg cagatggtcc gggccggcct
     5161 tcggatcggc gacgctgaaa cgccttccgt gctgcagcga cggcaccgcc ttgcgcgcgg
     5221 tctcgacttt tgcgggatcg atcgtggcca tgaacacgcc ggtctcaacg ccgccgccct
     5281 cggcaagcac tgtgccccag ggatcgacga tcagtgaatg gccgtagctc tggcgcttgc
     5341 tctcgtgcgt tccggcctgc gcggctgcga acacgaagca gccggtctcg atggcgcggg
     5401 cgcgcaacag cgtctgccaa tgcgcttcgc cggtcttgac ggtgaaggcg gaaggcaccg
     5461 tgaggaacga ggcgccgctt tcggcgagcg cacggtagag cgccggaaaa cgcatgtcgt
     5521 agcagattgt cagcccgatc cgtccccagg gcaggtcggc gagcacggcg gcttcgcccg
     5581 gctggtaatt ggcggattcg cgatagctct cgccgccggg cagatcgacg tcgaacatat
     5641 ggatcttgtc gtagctcgcg atcacgccgc catccggccc gatcaggaac gaacggttga
     5701 ccgcccgctc cggcgacgca cgcaacgcca gcgagccgat atggagatgg attttcaatt
     5761 ccgcggcgag cgcgcggtag gctttgagcg agagatcgtc ctcttccggg gcaagatttt
     5821 cgaacagcgc cttgcggttc gactggatca tgttggtcac ctcgggggtg agcacataat
     5881 cggcgccctg ggcggcggcc tgccgaatca gtcttgtgcc ctgctccagg ctcggccccg
     5941 gcagcaatcc ggtgcacatc tgcaccatgg cggcgttgaa ggagagctcc gcgctcatga
     6001 attggccttt tcgccggcca gcagcgcatc gagcttgccc tgccgatcca gcgcgtaaag
     6061 ctcttcacac ccgccgacat gcatctcgcc gacgaaaatc tgcggaaagg tcgagcccgc
     6121 gccggcgcga tcccacatct cttgccggcg tgccgggtcc ctggcgacgt cgaattcgat
     6181 gaaggcggcc ttcttgcgcg tcagcaacga cttggcggcg ccgcaatagc cgcagcccgg
     6241 acgagtatag atttcaatgg cagcggtcat ctcgcgctcg gtcttggcag gatcagccca
     6301 ttatatggga gctttatggg tgtccacaac ccgggcgaag accagcacgt ccacctgggc
     6361 cgccttggcg cgcaatagcg cccgcgcgca ggcgtcaacg gtggcgccgg tggtgaggac
     6421 atcgtcgacc aggacgatgc ggcggccggc aatgtcggcg ctacgatcgg ccgaaacctt
     6481 gaacgcgccc tgcacgttgc tcgcccgctg cgcgcgcgac aggccgatct gttgctgcgt
     6541 cggcctgatg cgccggagcg cttcggttgt cacgttcacg ccgctttgcc gctcgatcgc
     6601 gcgcgccagc gcgccggact ggttgtagcg ccggctccag cctcgctaca atcgcccgcc
     6661 taatcccggg gaacctcgca gagcatcaaa aagcccgcgt aaattgggcc tttctgttaa
     6721 ccccaatact agacgagttt tgacgaggtt cggcggggaa cctagttatg gaaaatagta
     6781 ggcgtaaaac gaggtattcc tgtgccggag aaattgctca aaaagaacga aatcgacggg
     6841 ctattgaaga agccggggaa gcaccgcgac ggcgcgggcc tgtatctgca aatcgcccgc
     6901 aggggccaag cgtcttgggt atatcaattt cggtggaatg ccgaaaccaa atggatgagc
     6961 attggaccgg cggcaacgtt caagcttgaa gaggcccgta agcgtcacca cgaattgcgg
     7021 cgcatgcggg acaggggtat tgatccgcgc tccagcgtgg cagcggtcgc gctccagccc
     7081 tatcagccta gcccgtcgat ggctccagcg gccccggacg ctccagagcc tccagagcag
     7141 cccgcaagcc cgctcttcgg cgaagtggtc gagttgttcc taaatgactt tgctgccgac
     7201 tgggcaggcg gtttcgaggg caaggaagcc aaatcatatc gccgtacctt gatcaccaaa
     7261 agcagcctcg caaagttcgc ggttgccgag atcagaaccg aacacgtcga agccgatcta
     7321 aagcaatggg ccgacaagcc ggtgacggcg gaaaaagtct tgatgcgtat cggcaaaatc
     7381 ttgaactacg cgtcggcgaa aaatttccgc tctaaagaag ttccgaaccc ggcacgcatc
     7441 aagggacact tcgaattttt ggcgcgtccc gtcgtgcccg agacggtgaa ccatcccgcg
     7501 atgaaaatac ccgacgttcc gggcttcatg cgagagctta ttgcggacgg ttcgatagag
     7561 gcccgggcgc tcgcggtctt gatcttgacc gggccgcgca ccgatgaatt gcggctggct
     7621 agatggaaag agatcgacgg cacaatatgg acaatcccgg cggagcgaat gaagggaaag
     7681 aagaaagacc gtcgcgcgca tagcgtgccg cttgtcccag cagttgtgaa gctgttgggc
     7741 aaacgcggcg ctgacaatga cttcattttc ccgtcgcgtc gctacggcgc tcgcaagccg
     7801 ctctggcatt cgtcactccg cgaagtgttg aataggttgc gcggtgacag gctttcgatt
     7861 gagggcttgc cgcctgtccc gcacggtttc cggtcaacgt tccgggactg ggcagccgag
     7921 gcagggtatc cccgcgactt ggccgaacgc gccatagctc acaaggtcgg cggcaagact
     7981 gagaccgctt atcaacgttc gaagctcatt gagcaacggc ggcccttaat ggttgcttgg
     8041 gccaaattct gcatttctgg aggccgccgc aatgactgac atcagggaag ccgagcggct
     8101 tgccctcatc actcacgctc aggtcgtact tgtcttggaa gaagccgaga acgccgaact
     8161 cgaagcccgc gcctttagtc tggaccgtgc tcgccagcgc cgcccttctg gcccgcacgt
     8221 ctgctccgtc attccctttc cgaagaggta ggtcgcaaac gctagcccat ctagaaatag
     8281 ccgccgccgg gttgagatcg gcgacggctt tccagaaagg cgggcgtgat acgcaacccg
     8341 ctggccaatg tcgccaaatc acgtgaataa agggtttagt caggtgactg atcgcggcga
     8401 cttgcgctat tggaaggttg agaagtctgg tttgggaaat atacgtcagt gggtctctgc
     8461 catatgcgcc ctaggatttg gccgaaaatc gtattcacct tgctcgccgc gtcggtagcg
     8521 atagcgtccg atggggcatc cgcgcagaca gtcaatctgg cgtgtggggg cgtatatcat
     8581 atgtacgcac cggaggagcc gatggatgct acccttgaac caacgtcgtc gtcggtagac
     8641 gtcgccaaca aaaggatcac caccccatta ggagagtatc gcatttcccg agttgaggat
     8701 gctcaacttt acttcgacaa gcctgcgagc cctactttca attttgtcac ccacggcacg
     8761 cttgatcgaa cgactggtaa aatgctgatc cggtggatgc gtcctgagga agacgccaag
     8821 gttcaggtgg ttggacagac ggcgcatatg gcgaggtacg ctgaatttac atgctctacg
     8881 gcgaaacgcc tgttctgaaa ttcggttcag aacctatgcc cgacaggaaa cgtcttgggt
     8941 tggatagccc cacagatttg ttggcgaagc ttcggtggga gatcgtgcag cttggccttc
     9001 ccatagatga gcaagccgtc gcatcatacc gggctttcaa ttgtgccgta acggcttgga
     9061 aaagagctcc gctccggccc gcatcgcttg agacggggga ggcgaaagct aagacgttta
     9121 tcctgggtgg ttgtcaaatt tgaggatcaa ccattgccta cattttta
//
LOCUS       DATNOI010000289         7463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2204069,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000289 DATNOI010000000
VERSION     DATNOI010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2204069"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..2503
                     /locus_tag="VN968_11295"
     CDS             80..2503
                     /locus_tag="VN968_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXL29860.1"
                     /translation="MIERRRIPASRSRLYRPALVAAGIGLLLSLAGASAVGRWEERVT
                     KVEFEGVAETQAIIMQNGVNEYVSRLAALRTLFESASEEITRSEFETFSGRLFEQHPG
                     ILRISWIPRINRKERAEYEAAAVGDGISGYRIKSLLADGGVATAPQGDEYFPVFFSTE
                     PKTSVAYGLDYSSDPERRATLERARDNELVATLRAELYESKGGIWAQGVLVCVPVYAK
                     GTSRATIADRRRNLAGFVVGIVSLPRLLGSIRTTTAATPAVRIDVYPSDMGGSAGPEN
                     RFVPDFSSEPGASQSAVADRPHWSGTLKIGDASWHVRATPTADGRLTTHHERALTVLA
                     AGIVITTFLAVYLGLASRNSRQLALANRRVLELAQTDILTGLPNRAFFLEQLEEADSY
                     ERRGSGAFSILMLDLDRFKNVNDSLGHAAGDALLRQVALRLKSAIRSTDALARLGGDE
                     FAIIQAECDDQRAGSIDLATRIAKLVAEPFLLQGNQVEVGTSIGIAMAPEHGSDQEQL
                     LKKADLALYRSKSAGRNCFTLYDEAMSAELEARNTLESDLRDAVARCQFELHYQPFFD
                     VQSGQRRGLEALVRWRHPTRGLIPPDQFIPLAEETGLIVPLGEWVLRRACDDATSWPA
                     DIKVAVNLSPVQLKQVELFDVIQSALRSSGLPAERLEIELTESVLLERAVENHALMER
                     LKSIGISLALDDFGTGYSSLSCLTAFPFDKIKIDRSFIGNLTKRHKSSAIISSIVTLA
                     RGLNLSVTAEGVETQEQFERLKTLGVNFAQGYLLGRPMPLGDPKVSLNQIMRTWVQLC
                     VSSRAMASG"
     gene            complement(2527..3897)
                     /locus_tag="VN968_11300"
     CDS             complement(2527..3897)
                     /locus_tag="VN968_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HXL29861.1"
                     /translation="MRSMLAATLIFVATASSAQAQMSGTATVAGVKPKPVTTIPIRPA
                     LQTPADTANAMAQAERQAIQSDLAWVGQYNGAITGEVSERMVAAIKEFQKERGGKPTG
                     VLNPQERGALAETARRRQDSVGWKIVTDAGSGVRLGIPTKLVPQQSSDANGARWSSST
                     GTIQIQLSRRKEANPTTAKLAEKEKKEPAGRVVDYTVVKPDFFVLSGLQGLKKFYVRG
                     TFKGDEVRILTILYDQATENTVEPVVIAMSSAFNPFPSGAQIAGPPPRKTVEYGTGIV
                     VSDDGAIVTDRQITDGCLAIAIAGFGNADRLAEDKDHDLALLRIYGARGLKPLGLGNG
                     AAKTGVELTGIADPQNQGGGAAASSVKATTTPIGSGNDLALAPPPAVGFSGAAAFDTD
                     GKFAGIALLKPVVVAGPPNAAPAAQATLVSADTVREFLRANAVNAADGPSEAKASVVR
                     VICVRK"
     gene            4141..4953
                     /gene="moeB"
                     /locus_tag="VN968_11305"
     CDS             4141..4953
                     /gene="moeB"
                     /locus_tag="VN968_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HXL29862.1"
                     /translation="MLSPEELERYARHIVLREVGGPGQAALKEASVLVIGAGGLGAPA
                     LMYLAAAGVGELGVVDDDIVSLSNLQRQIIHTTPDIGRRKVDSAAGQIHALNPHVRFA
                     AHATRLAADNAMELIGSYDLVLDGSDNFETRYLVSDACFLAAKPLISAALGMFDGSLT
                     TIRAHEKNAEGEFNPTYRCLFPEPPPPGTVPACAEAGVMGALAGVLGSMMALEAIREI
                     VGFGEGLVGRLLMVDARAMRFETLRYRRDPANPLNGDAPTIADLSGYLSGDL"
     gene            complement(5183..6188)
                     /locus_tag="VN968_11310"
                     /pseudo
     CDS             complement(5183..6188)
                     /locus_tag="VN968_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133997.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            6315..7225
                     /locus_tag="VN968_11315"
                     /pseudo
     CDS             6315..7225
                     /locus_tag="VN968_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(7325..>7463)
                     /locus_tag="VN968_11320"
     CDS             complement(7325..>7463)
                     /locus_tag="VN968_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HXL29863.1"
                     /translation="KVTLLPHMGSATIEGRVEMGEKVIINIRTFLDNHKPPDRVLPSM
                     L"
BASE COUNT         1290 a         2280 c         2499 g         1394 t
ORIGIN      
        1 cctgttcggc ggccactgcg cgtgtcgaac gaggacgaag atgggcgatc aagattctgg
       61 cgacacgtgc cagacagaca tgatcgagcg gcgccgaatc cctgcatcgc ggtcaagact
      121 ttaccggccc gcgctggttg cagcaggcat cggccttctg ctgtcattgg ccggggcatc
      181 tgcggttgga cggtgggaag agcgggtcac gaaagttgag tttgagggcg tagccgagac
      241 tcaggcgatc atcatgcaga acggcgtgaa cgaatacgtc agccggctgg ccgcgctgcg
      301 taccctgttc gaatcggcca gcgaggaaat cacccgcagc gagttcgaga ctttcagtgg
      361 gcgcctgttc gagcaacatc cgggcatcct tcgcatcagc tggatcccga ggatcaaccg
      421 gaaggagcgc gccgaatacg aagctgccgc ggttggcgac ggaatttccg gctatcggat
      481 caaatcgctc cttgcggatg gcggcgttgc gacggctccg cagggcgatg aatattttcc
      541 ggttttcttt tccacggaac cgaaaacctc ggtcgcttac gggctggact attcgtccga
      601 tcccgagcgg agggctacgc tggaacgcgc gcgggacaac gagctggtcg cgacgttgcg
      661 cgcagaactt tacgagtcga aaggcggtat ctgggcccag ggtgtgctgg tttgcgtccc
      721 ggtctacgca aaaggaactt cgcgcgccac gatcgccgac cggcggcgca atctggccgg
      781 ctttgtcgtc gggatagtca gtctaccgcg attgctgggg tcgatccgca cgacgacagc
      841 ggcaactccc gccgtcagga tcgatgtcta cccgtccgat atgggcggca gcgcgggacc
      901 ggaaaatcgg ttcgttcccg atttttcgtc cgagcccggc gcgtcgcagt cggcggtcgc
      961 ggatcggccg cactggtccg gcacgctcaa gataggcgac gccagctggc atgtgcgggc
     1021 cacgcccacc gccgacggcc ggctgacgac gcatcacgaa cgggctctga ccgtgctggc
     1081 ggccggaatt gtcatcacga cattcctggc cgtgtatctc ggtctcgcga gccgcaactc
     1141 gcgccagctc gcattggcga accggcgggt tctggagctt gcgcaaaccg acattctgac
     1201 cgggcttccg aaccgggcgt tctttctgga acaactggag gaggccgact cgtatgagcg
     1261 ccggggcagc ggggcgtttt caattctcat gctcgatctc gatcgcttca agaacgtcaa
     1321 cgactcgctt ggtcatgccg ccggcgacgc gttgctgcgg caggtggcgc ttcgtttgaa
     1381 atccgcgata cgcagtaccg atgcactggc gcggctgggg ggcgacgaat tcgcaatcat
     1441 ccaggcagag tgcgacgatc aacgcgccgg ttcgatcgat ctggcgaccc gaatcgccaa
     1501 actggtcgcc gagccattcc tgctgcaggg caatcaggtg gaagtcggta ccagtatcgg
     1561 tattgcgatg gcgccggaac acggcagcga tcaggagcaa ctgttgaaaa aggccgacct
     1621 tgcgctgtat cgatcgaaat cggccggccg gaactgcttc accctttacg atgaagcgat
     1681 gtcagcggaa ctggaagccc gaaacacgct ggagagcgat ttgcgggacg ccgtcgcacg
     1741 ctgtcagttc gagttgcatt atcaaccgtt cttcgacgtc cagagcggcc agcggcgtgg
     1801 cctggaagct cttgttcgct ggcgtcatcc gaccaggggg ctgataccgc ccgaccagtt
     1861 catcccgctc gccgaggaga ccggactcat cgttccgctt ggtgaatggg tgctccggcg
     1921 agcgtgcgac gacgcgacgt cctggccggc agacatcaag gtcgcggtga acttgtcccc
     1981 ggtccagctc aagcaggtcg agttgtttga tgtcatccag tccgcgctgc ggagttccgg
     2041 tttgccggcg gagcggctgg aaatcgagct cactgaatcc gtgctactgg aaagggcggt
     2101 cgagaatcat gcgttgatgg aacgactgaa gagtatcggg atttcgctgg cgcttgacga
     2161 cttcggcacc ggatattcat cgttaagctg cctgacggcg ttcccgttcg acaagatcaa
     2221 gatcgacagg tcgttcatcg gcaatctgac caagcgtcac aagagttcgg cgatcatttc
     2281 gtcgatcgtg acgcttgcgc gtggcctgaa cctgtcggtc actgccgaag gtgtggaaac
     2341 ccaggagcaa ttcgagaggc tgaaaacgct gggggtgaac ttcgctcagg gctacctgct
     2401 cggccggccg atgccgctcg gagacccaaa ggtcagtctc aatcagatca tgcgcacctg
     2461 ggtgcagctt tgcgtcagta gccgcgccat ggcgtcggga tgagcgcagt ggccaacagc
     2521 gcccggctac tttctcacgc agatcacgcg cacgacggac gcctttgcct ccgacggtcc
     2581 atccgccgca ttcacggcat tggctctgag aaattcgcgc acggtatcgg ccgacaccag
     2641 cgtcgcctga gccgcgggcg cggcattcgg cggtcccgcg acgaccaccg gcttcagcag
     2701 cgcaatgccg gcgaatttgc cgtcggtatc gaacgccgcc gcgccggaaa agccgaccgc
     2761 cggcggcggc gccagcgcca gatcgttgcc gctgccgatt ggcgtggtgg ttgccttgac
     2821 gctgctcgcc gcggcgccgc cgccctggtt ctgcggatcg gcaatgcctg tcagctcgac
     2881 gccggttttt gccgcaccat tgccgaggcc gagcggcttc aatccgcgcg cgccgtagat
     2941 gcgcagcagc gcgagatcat ggtccttgtc ctcggcgagg cgatcggcgt tgccaaagcc
     3001 ggcgatcgcg atggcgaggc agccgtcggt gatctggcgg tcggtcacga tcgcgccgtc
     3061 gtcgctgacc acgatcccgg tgccgtattc gacggtcttg cgcggcggcg ggccggcgat
     3121 ctgggcgccc gatggaaacg gattgaacgc gctcgacatt gcgatcacga ccggttcaac
     3181 ggtgttttcc gtagcctggt cgtagagaat ggtgaggatg cggacctcgt cgcccttgaa
     3241 cgtgccgcgg acatagaact tcttcaggcc ctgcaggccc gacagcacga agaaatcggg
     3301 cttgaccacg gtgtagtcga ccacgcgccc ggcgggttct ttcttttcct tctcggcgag
     3361 ttttgccgtg gtggggttgg cttccttgcg tcggctgagc tgaatctgga tggtgccggt
     3421 ggaggagctc catctggcgc cgttggcgtc gctggattgc tgcggcacca gcttggtcgg
     3481 gatgccgagc cgcaccccgc tgccggcatc ggtgacgatt ttccagccga cactatcctg
     3541 ccgccgcctg gcggtttcgg cgagcgcgcc gcgctcctgc ggattcagca cgccggtcgg
     3601 cttgccgccg cgttccttct gaaactcctt gatcgcggcg accatgcgct cgctgacctc
     3661 gccggtgatg gcgccgttgt actggccaac ccaggccaga tccgactgga tggcctgacg
     3721 ttccgcttgc gccatcgcgt tggcggtatc ggcgggggtc tgcaaggcgg gacggatcgg
     3781 aatggtggtc acgggcttcg gcttgacgcc cgccacggtc gccgttccgg acatttgagc
     3841 ctgggcggaa ctcgccgtcg ccacgaatat caatgttgct gcgagcatcg atctcatgac
     3901 aagtctcggg tccattgcag agcgcgcaat taagcacatc tgcttggtcg gcaacaggga
     3961 tgcggtggtt caagcgagcg atggtattct catccccggc cacgccggca aatatacgca
     4021 gccgcgcaag cttggctgcg agccgcgtct cgaaggatga cagacacgct tcatggttgg
     4081 ggacgcgcga cgacgcgctc cgcactgtga ggatcgacgg aaggtgagga acaacccgac
     4141 atgctgagtc ccgaagaact cgaacgctac gcccgccaca tcgtgctgcg cgaggtcggc
     4201 ggccccggcc aggccgcatt gaaggaagcg tcggtgctgg tgatcggcgc cggcggcctc
     4261 ggcgcgcccg cgctgatgta tctcgccgcg gccggcgtcg gtgagctcgg cgtggtcgac
     4321 gacgacatcg tgtcgctgtc caacctccag cgccagatca tccacaccac gcccgatatc
     4381 ggccggcgca aggtcgacag tgccgccggg caaatccatg cgctcaatcc ccacgtccga
     4441 ttcgcggcgc atgccacgcg gctcgcggca gacaatgcaa tggagctgat cggcagttac
     4501 gatctggtgc tcgacggatc ggataatttc gaaacccgct acctggtttc ggatgcgtgt
     4561 ttcctcgccg ccaagccgtt gatatcggca gcgcttggca tgttcgatgg atcgctgacc
     4621 acgattcgcg cgcatgagaa aaacgccgaa ggagagttca atccgaccta tcgctgtctg
     4681 tttcccgaac cgccgccgcc gggcacggta ccggcctgtg ccgaggccgg cgtgatgggt
     4741 gcgctcgcgg gggtgctggg atcgatgatg gcgctggagg cgatccgcga aatcgtcggt
     4801 tttggcgagg gcctggtcgg gcgattgctg atggtggacg cgcgcgcgat gcggttcgag
     4861 accctgcgct atcggcgcga tcccgccaac ccgctcaatg gcgatgcgcc gacgattgcg
     4921 gatttgagcg ggtatctgag tggggatctc tgaaggcccc caaccctccc cggcaagcgc
     4981 cggcaagcgg gagcggggca gtcagcagtc gttgcgaatg acgagaacaa acatcttcat
     5041 gagatttgca gcatacgtcc tcgatggttt cggacgccgc ttaccacgta tcacagcgaa
     5101 ctccctctcc cgcgtgcggt cgcgtgcggg gggagggtcg gggtgggggc cggctgcaag
     5161 cactgaaggt catgcgagca tcttacgccg tcttgcgctt ctcgctgtcg agatgggcga
     5221 gctgcgcgtc gggatagcgt cctccggccg cggcgccggg cgggaacgcc ttgggcaaga
     5281 tcggccaggt cggccgccgt caactttacc tcgagcgcgc cgagcgcttc ggtcaggcga
     5341 tcgcggcggc gggcgccgac cagcggcacg atgtcctttc cctgcgccag cacccaggcg
     5401 atcgcaacct gcgcgacgga ggcgccgata ttgccggcaa tcgcacgcag ccggtcgacc
     5461 agtgcaagat tggtgtcgag gtttttgtcc tggaaacgag gactgttgcg gcgaaaatcc
     5521 tgtgctccgg agcggtcctt cgaccaatgc ccgctgatca gaccgcgcga cagcacgcca
     5581 taggcggtga tgccaatgcc gaactcgcgg caggtcgcga gaatgtcgtc ctcgatgccg
     5641 cgtgcgatga gcgaatattc gatctgcagg tcggcgatcg gatgcacggc gtgggcgcgg
     5701 cgaatggtgt cggagccgac ctccgaaaga ccgatatatc tgacatggcc ggccttcacc
     5761 atctcggcga tggcgccgac cgtgtcctcg atcggcactg aagcatcgag ccgcgccggg
     5821 cggtagatct cgatatgatc gacgccgagg cgctgcagcg agtagctcag aaaattcttc
     5881 accgcggccg gccggcagtc catgccggca aaaccgtgga cgggctcgcg cagcgcgccg
     5941 aacttgacac tgatctgaac attgtcgcgg ctgcggccgg cgagcgcgtc gcggatcagc
     6001 aactcattgt ggccgacgcc gtagaaatcg ccggtgtcga gtagcgtgat ccctgcctcg
     6061 agcgcggcat ggatggtggc aatgctctcg ccgcggtcgg cggggccgta aaaatccgac
     6121 atgcccatgc agccgagacc gatggcgggg acgtttgggc cggtggcgcc cagcttgcga
     6181 tgttccatgg tggttctcct cgaaagcggc ccgctgacga tcccacgccg ccgttgtgac
     6241 gaggctgata tgaggtcatt tgattgaggc gataagctgg acaatgtcga atagcttgtt
     6301 cactatatcg aacaatgaaa gaactcgatc tccgcgatct cgatgccttt gtcgccgtgg
     6361 cgcgcacctt gaatttccgg cgcgcggcgc tggagcagcg cgtttcggtc tcgagcctga
     6421 gccagcggcg cgacatggaa gagcggcttg gggtccgcct gatgaaccgt accacccgca
     6481 gtgtcgcact gacggaggcc ggtgagttgc tgctggcccg tgtcggaccg gcgatggcgg
     6541 atgtcggtga tgcgctggat cgggtgcggg gcctgcgcga tgtgccgtcg gggcgcttgc
     6601 gcatcaatgc gccgccgccg gcgatcgatc tggtgctggc gccgatggcc gcgccgttcc
     6661 tcaaggccta tccgcaaatc gatctggaga tcgcgagcga cagttcgttc atcgatatcg
     6721 tcgcggccgg cttcgacgcc ggtgtgcgct acggcgaaca cctagcgcag gacatgatcg
     6781 cggtctcgct cggcccgccg caacgctacg cggtggtggc atcagcgcag tatgtcgcgc
     6841 agcacggccg gccgaaacag ccggaggatc tgctgggcca cgcctgcatc cgtacccggt
     6901 tcggcagcgg cgccatgctg gattgggagt tcgaaaaggc cggccgaaag tcgccccgcc
     6961 gccgagaatg atcgcgacct atctcggcct ggcgatgcgg gcggtccatg acgggcttgg
     7021 ctactggctg acgttcgaag gctatgtgcg cgatgctgtg agatccggcg cgctggtcag
     7081 cgttctcgaa gactggtgcg cgccgtttcc ggggccgttt ttgtattacc cgagccggcg
     7141 ccagccgcag cccgcactcg gcgccttcgt ggctttcgtc gcggaatggc ggaagcagga
     7201 gaggcggagg ggcaaggggg cttgagcctc acaatatcgg tgagggttca ggcgtggccc
     7261 agatgcagcg catcgccggg agaacgctgc accgcgtccg ggacacgtca actcgcgtta
     7321 cggatcacag catgctgggc agcacgcgat ccggcggctt gtggttgtcg aggaaggttc
     7381 ggatgttgat gatcaccttc tcgcccatct cgacacggcc ttcgatggtg gccgagccca
     7441 tatgcggcag cagcgttacc ttg
//
LOCUS       DATNOI010000290         4138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_938345,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000290 DATNOI010000000
VERSION     DATNOI010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938345"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VN968_11325"
     CDS             complement(<1..524)
                     /locus_tag="VN968_11325"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HXL29864.1"
                     /translation="MARMAKQINAVFAADKTAALALTPVSRETEARLDRYVDLLLQWQ
                     AKTNLVAPSTLPTLWTRHIADSLQLLALAPAAKVWVDLGSGGGFPGVVLACALAETSG
                     AMLHLVERNAKKAAFLREALRVTGARGMVHLTEIGDTVDRITGPVDCVTARAVAPLNK
                     LIGFAEPLVRKGAKA"
     gene            complement(682..2565)
                     /gene="mnmG"
                     /locus_tag="VN968_11330"
     CDS             complement(682..2565)
                     /gene="mnmG"
                     /locus_tag="VN968_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HXL29865.1"
                     /translation="MTTRQESFDVIVIGGGHAGCEAASAAARMGARTVLVTHRFATIG
                     AMSCNPAIGGLGKGHLVREVDALDGLMGRVADAAGIQFRMLNRRKGPAVRGPRAQADR
                     KLYAAAMQAAITETANLGVIEGEADDLIVSDGRVAGIRLADGRELSATAVVITTGTFL
                     RGLIHLGEKNWPAGRVGEAPALGLSKSFERAGFTLGRLKTGTPPRLDGTTIDWSAVEM
                     QPGDDPPEPFSVMTEEITTPQIQCGITRTTAATHEVIRANVHRSPMYSGQIKSTGPRY
                     CPSIEDKIVRFGDRDGHQIFLEPEGLDDTTVYPNGISTSLPEEVQLQILASIPGLERV
                     RMVRPGYAIEYDHVDPRELDPTLQTRRLPGLFLAGQINGTTGYEEAAAQGLVAGLNAA
                     LAAAGSAPFVFDRADGYLGVMIDDLVTRGITEPYRMFTSRAEYRLTLRADNADQRLTD
                     NGIALGCVGQARSVRHRAKMTALNAAKALAKSLTLTPNEATRFGLALNRDGHRRSAFE
                     LLAYPEIGWAEICGIWPELSAIEPSIAVHLEIDAKYDVYLKRQVADVAAFRRDEGLIL
                     GDIDYRLVPGLSNEARSKLEAARPRTVGQAGRLDGLTPAALGILAAYLRREARRKTAL
                     ASA"
     gene            complement(2710..4071)
                     /gene="mnmE"
                     /locus_tag="VN968_11335"
     CDS             complement(2710..4071)
                     /gene="mnmE"
                     /locus_tag="VN968_11335"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HXL29866.1"
                     /translation="MYPREQTIFALSSGRPPSAISIVRVSGSQAGTALISLAGRMPLP
                     RMATRALLRNADRQPIDDAVVLWFPGPGSATGEDVAEFHVHGGRAVLAALFAALSGFA
                     DIRPAEPGEFARRAFENGKLDLTEAEGLDDLIRADTDRQRRQALRQLKGLLGDKARDW
                     RAQIIEASALIEAGIDFSDEGDVPAELIAPALAKIKTLLGEIQEVLAGQGRSERLRDG
                     LVVAIAGPPNVGKSTLMNQLARREVAIVSPHAGTTRDIIEVQLDLDGYPVTVIDTAGI
                     RETEDPVEQEGVRRARTRAAEADLVLWMIDSQHEQSPQQGAAPTWTVRNKIDLDAVRA
                     DSPKPRQAGAVGQERRLGSDFGISASRGDGIPELISALVAFARDYLGSGEEGLISRAR
                     QRQLLQETAGSLQRSMEVVGKGEELAAEELRAAAHSLGRLLGRVDVEDILDAIFRDFC
                     IGK"
BASE COUNT          735 a         1392 c         1285 g          726 t
ORIGIN      
        1 gcttttgcac cctttctcac cagcggctcg gcaaaaccga taagtttgtt tagcggagcg
       61 accgctcggg cggtcacgca atcgacaggg ccggtgattc tgtccacagt atccccgatc
      121 tcagtcagat gaaccatccc ccgtgcgccg gtaacgcgga gggcctcgcg caggaacgcg
      181 gccttcttgg cattgcgctc caccagatgg agcatcgcgc ctgatgtttc cgccagcgcg
      241 caggccagca ccacgccggg aaaacctccg ccgctgccaa gatcgaccca gaccttcgcc
      301 gcgggtgcga gagccaaaag ctgcagcgaa tccgcaatgt gccgggtcca cagagtgggc
      361 agcgtcgagg gcgccacaag attggtcttc gcctgccatt ggagcagcag gtcgacatag
      421 cgatccagcc gcgcctccgt ttcacgtgaa accggcgtca gggcgagggc cgcggtcttg
      481 tcagcggcga aaactgcgtt gatctgtttt gccatcctgg ccatttgctc gatggtagtc
      541 gtcgtcctga ggtgtgagcc ctcttcggcg agcctcgaag gacggcgacg agtgttgtgg
      601 accgtccttc gagacggcgc tacgcgcctc ctcaggatga cgttaggagt acgacgcaac
      661 cctgtttcac gtgaaacgga cctacgccga ggccagcgcc gtcttccgtc gcgcttccct
      721 tcgcagatag gccgcaagaa tgccgagcgc ggccggagtc aggccgtcga gccgacccgc
      781 ctgccccacc gtccgcggac gcgcagcctc gagctttgaa cgggcctcat tggaaagccc
      841 ggggaccagg cggtaatcga tatcgccgag aatcagcccc tcgtcgcgcc ggaaggccgc
      901 gacatcggcg acctgacgct tgagatagac gtcgtatttg gcgtcgatct ccagatgcac
      961 cgcgatcgat ggttcaatgg ccgacagctc cggccagatg ccgcaaatct ccgcccagcc
     1021 aatctccgga taggcaagca gctcaaaggc cgagcggcga tggccgtccc ggttgagcgc
     1081 tagcccaaac cgcgtggctt cattgggcgt aagcgtcaac gacttcgcga gcgccttcgc
     1141 ggcattcaac gccgtcatct tggcgcggtg gcggaccgac ctggcctgcc ccacgcagcc
     1201 cagcgcgatt ccgttgtcgg tcaggcgctg gtcggcgttg tcggcccgca aggtcaggcg
     1261 gtattcggcc cgcgaggtga acatccggta cggctcggtg atgccgcgcg tcacgagatc
     1321 gtcgatcatg acgccgagat agccatcggc gcggtcgaat acgaacggcg cagaacctgc
     1381 cgcggccaag gctgcattga ggccggccac aagcccctgc gccgcagcct cttcatagcc
     1441 ggtggtgccg ttgatctgtc cggcgagaaa aagacccggc aaacgcctgg tctgcagcgt
     1501 cggatcgagt tcgcgcgggt cgacatggtc gtattcgatg gcatagcccg gacggaccat
     1561 ccgtacgcgt tcgaggccgg gaattgacgc cagaatctga agctgcacct cttccggcag
     1621 cgaggtcgaa atgccgttgg gatagaccgt tgtgtcgtcg agtccttccg gctccaggaa
     1681 gatctgatgt ccgtcgcggt cgccgaagcg gacgatcttg tcctcgatcg agggacaata
     1741 gcgcggtccg gtgctcttga tctgtccgga atacatcggc gagcgatgca cattggcccg
     1801 gatcacctca tgggtagcgg ccgtggtccg cgtgatgccg cactggatct gcggggtggt
     1861 gatctcctcg gtcatcaccg aaaacggctc cggcggatcg tcgcctggct gcatctccac
     1921 cgcggaccag tcgatcgtgg tgccgtcgag gcgcggcggc gtgccggtct tcagccggcc
     1981 cagcgtgaag ccggcgcgct caaacgactt cgagagcccg agcgccggcg cctcgccgac
     2041 gcggccggcg ggccagttct tctccccgag atggatcagc ccccgcaaaa aagtcccggt
     2101 ggtgatgacg accgcggtcg cggatagttc gcggccgtca gccaggcgga tgccggcgac
     2161 gcggccgtcg gagacgatga ggtcgtccgc ctcgccttcg atcacgccga gattggcggt
     2221 ctcggtgatg gcggcctgca tcgccgcagc atagagcttg cggtcggcct gggcgcgcgg
     2281 gccccgcacc gccggaccct tgcgccggtt gagcatgcga aactggattc cggcggcatc
     2341 ggcgacccgg cccatcagcc cgtcgagcgc gtcgacctcc cggaccaggt ggcccttccc
     2401 gagcccgccg atcgccggat tgcaggacat tgccccgatg gtcgcgaacc gatgggtgac
     2461 gagcaccgtc cgggcgccca tccgcgctgc ggcggacgcg gcttcgcaac cggcgtggcc
     2521 tccgccaatg acgatgacat cgaatgattc ttgccgggtg gtcatggcgg acttctatcg
     2581 cgggacggga aaacccggaa gcggaagttg gccgttgaga aggtcgtttc acgtgaaaag
     2641 caatactccg ggcaacgggg tatctgtttc acgtgaaaca agtcagacaa agaaatgaac
     2701 caagaactat tactttccga tacaaaagtc tctaaaaatc gcatccagaa tgtcttccac
     2761 atcgacccgg ccaagcagcc gacccaagga atgagctgca gcccgcaact cttccgctgc
     2821 cagctcctcg cccttgccga ccacttccat gctgcgctgc agcgaacccg ccgtctcctg
     2881 tagcaattgt cgttgccgcg cccggctgat caggccctcc tcgcctgagc ccaggtaatc
     2941 ccgtgcgaac gcaaccagcg ccgagatcag ctccgggatc ccgtcgccgc ggcttgcgga
     3001 gattccgaaa tcggagccaa gccgcctctc ctgcccgaca gctcccgcct gacgtggctt
     3061 cggcgaatca gcccggacgg catcgagatc gatcttgttt cgcaccgtcc atgtcggcgc
     3121 ggcaccttgc tgcgggcttt gctcgtgctg tgaatcaatc atccacaaca ccaggtcagc
     3181 ctccgccgcg cgcgttcgcg cgcgacgcac gccttcctgc tccacgggat cctcggtctc
     3241 acgaattcca gcggtgtcga tcaccgttac cggatagccg tcgagatcga gctgcacctc
     3301 gatgatgtcg cgcgtggtgc cggcatgcgg cgagacgatc gcgacctcgc gccgcgccag
     3361 ctggttcatc agcgtcgact tgccgacgtt cgggggaccc gcgatcgcaa ccaccagccc
     3421 atcccgcagc cgttcgcttc ggccctgtcc cgcaagcact tcctggattt caccgagcag
     3481 cgttttgatt tttgccagtg ccggcgcgat caattcagcg ggcacgtcgc cttcgtcgga
     3541 gaaatcgatc ccggcctcaa tcaatgcgga ggcttcgatg atctgcgcgc gccagtcgcg
     3601 ggctttgtcg ccgagcagac ctttcaactg acgcaatgcc tggcggcgct gccgatcggt
     3661 gtcggcgcgg atcagatcgt cgaggccttc ggcttcggtg agatcgagct tgccgttctc
     3721 gaacgcgcgc cgcgcgaact cgcctggctc cgccggacgt atgtcagcga aacccgacag
     3781 cgccgcgaac agggccgcca gcaccgcccg cccgccatgg acgtgaaact ccgcgacgtc
     3841 ctcgccggtc gcactcccgg gtcccggaaa ccacagcacg acggcgtcat cgatcggctg
     3901 ccgatccgca ttccgcagca aggcccgcgt cgccatccgc ggcaagggca tccggccagc
     3961 gagcgagatc agcgcagtcc cggcctgcga cccggatacc cgcacaatgg aaatggcgct
     4021 tggcggccgg cccgatgaaa gcgcgaagat ggtctgctcc cgcggataca tcgcctatct
     4081 gttccagagc cctttgcgaa ggcaatgcaa tttccgcttt gccgatttca gacggact
//
LOCUS       DATNOI010000291         4440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1099241,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000291 DATNOI010000000
VERSION     DATNOI010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099241"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1166
                     /locus_tag="VN968_11340"
     CDS             255..1166
                     /locus_tag="VN968_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXL29867.1"
                     /translation="MNQFAGVNLSRRKWLAPAVAGLFCLASLPCLAAEEPDVPKDGPK
                     GAAVTVLKAAKSCFSNIVEVSGILIPKEETSVRPERPGLKVAEILADAGDTVTAGQTL
                     ARLTLPEGGTQQLQAPVAGLISASTAVVGAQASGKGEALFSIIARSEFDLVGLVPVRD
                     ISKLAVNQTARIKIIGAGEVDGKVRRVATTIEPNSQLGQVFVSVTTNRRLLVNSSARA
                     LIKTGQSCGVSVPLTAILYGSAGTVVQVVRRQRVETRRVEIGLMSGGQVEVREGLQEG
                     DIVVARAGALLREGDPVRPVTASAEGK"
     gene            complement(1340..2206)
                     /locus_tag="VN968_11345"
     CDS             complement(1340..2206)
                     /locus_tag="VN968_11345"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXL29868.1"
                     /translation="MNVGQLRISRRSVTLAAAFVCVLSLAIGAHAALNKRALDAAKAI
                     EAEQTGALKGTSRLALVIGNGNYPDAAAPLAQPINDARALTGALRRNGFDVDVVEDAS
                     KDDMSRAIDRLKSKIKRDSVVMLFFGGYGAQVGRESYMLPVDAVIWKESDIRRNGFSV
                     ESVLDLMKEQGARTKLVVVDASRRNPYERRFRKYSHGLAPISAPDNALILTSATPGKV
                     ADDSTGQYSVLMTELLNNLNAPAAGIETVFNKTRAAISRASEGEQVPTVSSSLSEDVS
                     FGAKETAEKAGG"
     gene            2641..4374
                     /locus_tag="VN968_11350"
     CDS             2641..4374
                     /locus_tag="VN968_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29869.1"
                     /translation="MNLHRIAGAAYCALTARKDGPSLYDVCDPLLLNHAGGDPHLAKF
                     YRTALANPALRPLLRRTGLAELRDEARLQRLREALIRARDDAEPDWAAVGQPVADLLD
                     TIHLQHPRPKPAPVPARAPDLGEIESVIRTSAAHLLRSFGKNGFIPTYAAFNLIGDPD
                     IGGRELLMALTGLNARGYKNSTLLFNLARIFIARSPAAALINPSWTGIAEPMWQPMQI
                     RHRSAYYDAFFTEALLSFVETGLASQDQTTASRRAIAEMVDFCLTTSREDVLAQDGEA
                     VSVVTALAPLPHPRFSRFFAQIKQDLGFGVYVPDCDTTACSFSAATQAGSDNPILTQP
                     LLDFYAGLQVGPGDNAPRVTVPLNNNIDYEGGVVTWIDNLAGERPFGNDLDPTLNLDI
                     LEVSFRNLSRWKVLETPQRLDTVHRILGFQKRLAAGGAFTDPRSHIYYLPELYCAYFG
                     RCYSAFITLPAAARQAIDPDGAFEFIRARVLAYVQDELITGEMNAFDAALALIALGHL
                     GADAKTLTPALHCIVSSLGEGGRHGPFKGYEWNKMKTPTRILVGGPEVTSAFVLMGLA
                     LARRAMMRGAG"
BASE COUNT          744 a         1455 c         1452 g          789 t
ORIGIN      
        1 gatgcggagg cgatcgacgc ggcaaagccg cttgtcgatg gtcgcgatat tgagctgtgg
       61 agtggcgata ggctcgtcac gaggctggaa gccaacacaa agtaatcacg cgcggtgagc
      121 cgttttgaaa agggcttatc gcgggccgtg ctctggaatg acgccggccg agccgctccg
      181 acgctccatt gccgcttggc tcccaagccc gccttctgat atcgaagacc cccgaagacg
      241 agactggatc gccgatgaac caatttgccg gggtcaacct ttcgcgaaga aaatggctcg
      301 cccctgctgt cgccgggctg ttttgcctgg cgtcgctgcc atgcttggct gccgaggagc
      361 ccgatgtgcc caaggatggc cccaagggcg ccgcggtcac cgtgctcaaa gccgcaaagt
      421 cgtgcttctc caacatcgtc gaggtttccg gcatcctgat tccgaaagag gaaacctcgg
      481 tgcggccgga gcggccgggg ctgaaggtcg cggaaatcct ggccgatgcc ggtgacaccg
      541 tgaccgccgg ccagacgctg gcccggctca cgctgcccga aggcggaacg cagcagctgc
      601 aggcgccggt ggccgggctg atctccgcct ccacggccgt ggtcggcgcc caggcctcgg
      661 gcaagggcga agcgttgttt tcgatcatcg cgcgcagcga attcgatctg gttgggctgg
      721 tgccggtgcg ggatatttca aagctcgcgg tcaaccagac cgcgcggatc aagatcatcg
      781 gcgccggcga ggtcgacggc aaggtacgcc gggtcgccac cacgatcgag cccaacagcc
      841 agctcggcca ggtgttcgtc tcggtgacca ccaaccggcg cctcttggta aactcgtcgg
      901 cgcgggcgct gatcaagacc gggcaaagct gcggcgtgtc ggtgccgctc accgccattc
      961 tctatggcag cgccggcacc gtggttcagg tggtgcggcg gcagcgggtc gagacccggc
     1021 gggtcgagat cgggttgatg tccggcggcc aggtcgaagt ccgcgagggc ctgcaggaag
     1081 gcgacatcgt ggtcgcccgc gccggcgcgc tattgcgcga aggcgatccg gtgcgcccgg
     1141 tgacggccag tgccgagggg aagtgagggg gaggacttgg caggattgac tgagttcttg
     1201 tcattccccg atgtgcaatt gcacatcggg gaatgcgcca cttggcgcag gcccggaatc
     1261 catactcccg accgtggtta tggattccgg gctcgcgcta acgcgcgccc cggaatgaca
     1321 gcgcctaatg aacgagactc taaccgccgg ccttttcggc ggtttccttg gcgccgaagc
     1381 tgacgtcttc cgagagcgac gacgacaccg tggggacctg ttcgccttcg gaggcgcggg
     1441 agatcgcggc gcgggtcttg ttgaagacgg tctcgatgcc ggcggccggc gcgttgaggt
     1501 tgttgagcag ctcggtcatc agcacgctgt actggccggt ggaatcgtcg gccaccttgc
     1561 ccggcgtcgc cgaggtcagg atcagcgcgt tgtcgggcgc gctgatcggc gcgaggccat
     1621 gggagtattt gcggaagcgg cgctcgtaag ggttacgccg ggaggcgtcg accacgacca
     1681 gcttggtgcg ggcgccctgc tccttcatca ggtcgagcac ggattcaacg ctgaagccgt
     1741 tgcggcggat gtcggattcc ttccagatca ccgcgtcgac cggcagcatg tagctctcgc
     1801 ggccgacctg agcgccatag ccgccgaaga acagcatgac cacggaatcg cgcttgatct
     1861 tcgatttcag ccggtcgatc gcccggctca tgtcgtcctt gctggcgtcc tcgacgacgt
     1921 cgacgtcgaa tccattgcgg cgcagcgcgc cggtcaaggc ccgggcgtcg ttgatcggct
     1981 gggccagagg tgctgcggcg tcgggataat tgccgttgcc gatcaccaaa gcgagccgcg
     2041 aggtgccctt cagggcgccg gtctgctcag cctcaatggc cttggccgca tccagcgcgc
     2101 gcttgttcag cgcggcatgg gcgccgatcg cgagcgacag cacgcatacg aaagcagcgg
     2161 ccagggtgac cgagcggcgg gagatacgaa gctgacctac attcatcgcg ttcgttcctg
     2221 ctcattagtc ccggcttgct cgatcgtcca agccggaccg ttgtcaaagg cgcgcggagt
     2281 atgtcgcgcc aaagccatcg ggggtttgag ggggtggctg atcgtgtgcc gccgaattgc
     2341 gctcaatttg cggcgccact acttcaattt gtggcagcgc agcaaacaaa ccgttaaccg
     2401 gcaacggctt tgtgggcaat gctggcaccc gcttttggtt caccggaact ccccaattgt
     2461 ggcatccggg caacgcccga tggaactcgg cgtctgtaat ccagatcaca ccggtattct
     2521 cttactaatg atatcaattt caatgacttg cgggtgtccc gcgggcgcgg cagccatggg
     2581 tagtggattt ggcgggcccg gcgggacgtg agacaacgga ctggatagaa agaaactgta
     2641 atgaatcttc atcgcatcgc cggagccgcc tactgtgcct tgacggcccg caaggacggc
     2701 ccatcgctct acgacgtctg cgaccccctg cttctgaacc acgccggcgg cgacccgcac
     2761 ctcgccaagt tctaccggac cgcgctcgcc aatccggcgc tgcggccgct gctgcgccgg
     2821 accgggcttg ccgaactgcg cgacgaggcg cgcttgcagc gcttgcgcga ggcgctgatc
     2881 cgggcgcgcg acgacgcgga gccggactgg gcagcggtcg gccagccggt cgcggacctg
     2941 ctggacacca tccatctgca gcacccccgg cccaagccgg cgccggtacc ggcgcgcgcg
     3001 cctgaccttg gcgagatcga aagcgtcatc cgcacctctg ctgcgcatct gttgcggtcg
     3061 ttcggcaaga acggctttat cccgacctat gccgccttca acctgatcgg cgaccccgat
     3121 atcggcggcc gcgaattatt gatggcgctg accgggctga atgcgcgcgg ctacaagaat
     3181 tcgacgctgc tgttcaacct ggcgcggatc ttcatcgccc gctcgccggc ggcggcgctg
     3241 atcaacccct cctggaccgg catcgccgag ccgatgtggc agccgatgca gatccgccat
     3301 cgctccgcct attacgacgc gttcttcacc gaggcgctgt tgagctttgt cgaaaccggc
     3361 cttgcctctc aggaccagac cacggcgtcg cgccgcgcca tcgccgagat ggtcgatttc
     3421 tgcctcacca ccagccgcga ggacgtgctg gcgcaggacg gcgaggcggt cagcgtcgtc
     3481 accgcgctgg cgccgctgcc gcacccgcgc ttttcgcggt tcttcgccca gatcaagcag
     3541 gacctcggct tcggggtcta cgtgccggat tgcgacacca ccgcctgttc gttctcggcg
     3601 gcgacccagg ccggctccga taacccgatc ctgacgcagc cgctgctcga cttctacgcc
     3661 ggcctgcagg tcggccccgg cgacaacgcg ccgcgggtga cggtaccgct caacaacaac
     3721 atcgattacg aaggcggcgt cgtcacctgg atcgacaacc tcgccggcga acgtccgttc
     3781 ggcaatgacc tcgacccgac cctcaatctc gacatcctcg aagtcagctt ccgcaacctg
     3841 agccggtgga aagtgctcga gaccccgcag cggctcgaca ccgtgcatcg catcctcggc
     3901 tttcagaaac gcctcgccgc cggcggcgcg tttaccgatc cgcgctcgca catctattat
     3961 ctgccggagc tttactgcgc ctatttcggc cgctgctact cagccttcat cacgttgccg
     4021 gcggcggccc ggcaggcgat cgaccccgac ggcgcgttcg aattcatccg cgcccgggtg
     4081 ctcgcctacg tgcaggacga gttgatcacg ggcgagatga acgcgttcga tgcggcgctg
     4141 gcgctgatcg cgctcggcca tctcggcgcg gatgccaaga ccctcacccc ggcgctgcat
     4201 tgcatcgtca gctcgctcgg cgaaggcggc aggcatggac cattcaaagg ctatgaatgg
     4261 aacaagatga agaccccgac gcgcatcctg gtcggcggcc ccgaggtcac ctcggccttc
     4321 gtgctgatgg gcctcgcgct ggcgcgccgg gccatgatgc ggggggcggg ctagttgagg
     4381 tttcgcgctg ctccaacgtt ggctgtcata ccccgcgaaa gcggggtacc cagtacgccg
//
LOCUS       DATNOI010000292        14955 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1802830,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000292 DATNOI010000000
VERSION     DATNOI010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 14955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14955
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802830"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..81
                     /locus_tag="VN968_11355"
     CDS             <1..81
                     /locus_tag="VN968_11355"
                     /inference="COORDINATES: protein motif:HMM:NF023816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29870.1"
                     /translation="KEIVENPEVIRAYLGSYVHPPTPGET"
     gene            81..785
                     /locus_tag="VN968_11360"
     CDS             81..785
                     /locus_tag="VN968_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL29871.1"
                     /translation="MLKLSGVSASYGSVPAIGNVNIEIGEGEAVGLLGANGAGKSTSL
                     RVISGLVKLTSGTITFAGSNLASLPPHKIPELGIAHVPEGRQVFPEMTVQENLEIGAY
                     VPKAKAERSRTLELVYSIFPRLADRKKQLAGTMSGGEQQMLAVGRGLMLKPRLLMLDE
                     PSLGLAPVMTDVTFEKIGEIHKMGTAILLVEQNVSRALSLVQRAYVLESGNVIMHGTS
                     AELAGNKQVQAAYLGI"
     gene            complement(987..1808)
                     /locus_tag="VN968_11365"
     CDS             complement(987..1808)
                     /locus_tag="VN968_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXL29872.1"
                     /translation="MTPTRRHVLTALAAAAAAKAAAAHGGYLQEIGLQLYTVRDLLST
                     DFEGTLRAIARLGYQEVEFAGVLGPDLKQTRNLLGELGLSAPSLHIDYNSLRKNTEAS
                     FETATALAAKFVVCPSVDQSERQTIDDWKRVCENLNSIGELATHSGLTFAYHNHNFEF
                     SSLPNGVSPYDFLLANTNDRFVKFELDVYWAKKGNVDPVAYLKAYQSRFRLVHLKDMA
                     TDGSFTEVGNGTLDFDEIIAASIAAGVRHFFVEQDYSSDPMRSIAQSIAYLQRYR"
     gene            complement(2085..3966)
                     /locus_tag="VN968_11370"
                     /pseudo
     CDS             complement(2085..3966)
                     /locus_tag="VN968_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846505.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
     gene            3857..4102
                     /locus_tag="VN968_11375"
     CDS             3857..4102
                     /locus_tag="VN968_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29873.1"
                     /translation="MIRAFSSVPRKPRDASSKSRVSENGNAFSVAACCATTDAEASLG
                     TSLLPVWVIELFSPAWCERADYDELTSIVKAFGTTAQ"
     gene            complement(4293..5312)
                     /locus_tag="VN968_11380"
     CDS             complement(4293..5312)
                     /locus_tag="VN968_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL29874.1"
                     /translation="MKAFGIKAFGIAGLALALLAPSLPAGAAEPVNLILNWTPTADHS
                     PFYYAKAQGWYEKAGIDLNIEVGKGSGVSSLKVGSGGSPLGIADLATMLVARGKGADV
                     VALMSIYANTGQTFYWLKSYGVNGAKEFPGHKIGNPPGDASRVMWPAFAKAAGIAPDA
                     VGFVNVGPTAKIAALKSHTVDIISDFYNEHDLKVIEFGSDLGFVNWKDIGLNPYGNSL
                     IVNGAFLEKNPKLAEDFVKISQKAFAACVADASPCLKALLDQVSGLDKENQERQWERI
                     KFLMTDDFTTTKALGWIDAARMKKDYELVQTYLGMEKPFAVETAFTTKLLDPSIKMDA
                     SKVKK"
     gene            5439..6101
                     /locus_tag="VN968_11385"
     CDS             5439..6101
                     /locus_tag="VN968_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL29875.1"
                     /translation="MPKQRSPKRRSNGNKPQRRDPVATRKKLLTAARREFAQSGLAGA
                     RVDEIADRAGVNKQLVYHYFGDKDALYLAVLEWVYEEIRAQERKLNLEGLPPERAIKK
                     LIESSFDHLAAHPDFIVLLNDENRGGARHVRGSRKIETMHSPLVSMVSKILKQGVRAG
                     SFRKGINPVHLYISIAGLSYFFFSNTPTLSAIFGKDLASPAAKRTRRKHVVDLVMQSL
                     RP"
     gene            6333..7103
                     /locus_tag="VN968_11390"
     CDS             6333..7103
                     /locus_tag="VN968_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL29876.1"
                     /translation="MSSDGIRSARSFAIILIVHLVVIVLWQVLVDAFHVPKFVLPSPL
                     ATVATLTSAKYAWLSNLAVTAAEILGGFALGALVGVVLAVLFCWSPLVSLLLLPLFVT
                     LNMIPKVALGPLFIVWFSYGIFPNILIAFSICFFPILLTTARGLSEVEPDLLDLVKSL
                     RGSRWTLFRKIQLPGALPYVFSGMKVGAILAVAGAIVGEFIASERGLGYLMIQVQSSL
                     DTPAMVMAVVLLTLLGVALYGLVLGLERLFVVRDVRLQ"
     gene            7123..8160
                     /locus_tag="VN968_11395"
     CDS             7123..8160
                     /locus_tag="VN968_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL29877.1"
                     /translation="MLLTRENLPDTIPDIAALDDLLCRPSQALIDDLQKVDGDIMILG
                     VAGKMGPTLAGLAKAALPHRRIIGVARFSDASAKAWLEARGVETLNCDLLDEAAIKAL
                     PKVKNIVFMAGRKFGAEGDLSLTWAMNAHVPALVAQAFVQSRIVAFSTGCVYPFVPVN
                     GNGADETLAPNPPGEYAQSCVGRERMFEYFSRRFSTPGRLFRLNYAIDMRYGVLHDIA
                     TKILGGKAIDVSLGHVNFIWQGDASAQALRCLAHCDTPTSPINVSGHEILSVRDLAAI
                     LGAFLGRDPIITGTEQPTAWLTDTSQAAKLFGPPIVDTARLIAWTADWVARAMPSLGK
                     PTKYEVRDGRY"
     gene            8147..8980
                     /locus_tag="VN968_11400"
     CDS             8147..8980
                     /locus_tag="VN968_11400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL29878.1"
                     /translation="MAGTDHMAIVQLGPDDALAGLALSTEAHWNQNEADWRFFLSQGT
                     VFGVRDHDGHLVASAALLPYTSGNAWISMVLVTAGWRRRGLATRLVDACIDTAAKQGF
                     TSWLDATPDGATVYAPLGFAPTFELRRLRLEILAVPKATTPPSLSSYSLDEFITRDIS
                     TMGFNRSALLAALGGRPGSRLVSSSSAAALVRDGRKARHIGPLFADSPDRALALVHEI
                     IRSETGPILIDAVGTQDEFLEGLTSSGWTIERPFQRMRFGGATARPTEPPFAVAGPEY
                     G"
     gene            8992..10047
                     /locus_tag="VN968_11405"
     CDS             8992..10047
                     /locus_tag="VN968_11405"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXL29879.1"
                     /translation="MHHGAMNSKVRRLIADGTVIPAHPLALDANRTLDSVHQRALTRY
                     YIDAGAGGLAVGVHTTQFAIRDVGLYRPVLELAAETAANWTRRPLALVAGLAGSTRQA
                     IAEAEIARGIGYHAGLLSLAAMKSASEDEIITHCEAVAREIPLVGFYLQPAVGGVILS
                     ANFWRRFAAIDNVIAIKIAPFNRYRTLDVLRGVCAAGALDRIALYTGNDDHILLDLAL
                     PFDLRDRGVTVRTYFRGGLLGHWSVWTETAIKQFEMCRAARHKDTVPSDLLALDARVT
                     DCNSAFFDVANNFHGCIAGCHEILRRQGLLEGIWCLDPAEGLSPGQIQEIDRVCNEHA
                     DLADDDFVADHLQKWLA"
     gene            10035..10820
                     /locus_tag="VN968_11410"
     CDS             10035..10820
                     /locus_tag="VN968_11410"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL29880.1"
                     /translation="MAGMTEAPFIRLRNVRKVYRSQGVEFLAVSDVTMDVREGELISL
                     VGPSGCGKTTVLKILAGLHGADGGTIQIGNARTPFDPARDIGMVFQQALLLKWRTVLD
                     NVLLPAEIVGLPIKAARARARDLLNLVGLAGYEEKYPQQLSGGMQQRTAIARAFIHDP
                     KLILMDEPFGALDALTREQMNLEMLRIWRESGKTIIFVTHSIHEAVFLGSHCAVLTAG
                     PARMAEYFPIDLPFPRALPVKTTDEFGAYARRIYSSLGLGPSA"
     gene            complement(10912..11964)
                     /locus_tag="VN968_11415"
     CDS             complement(10912..11964)
                     /locus_tag="VN968_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL29881.1"
                     /translation="MIDNSYYSQAVHGPYELYDIGNLELEEGGTIRACNLAYATFGKL
                     NAAKTNAILIPTWYSGTNKIIEQVYIGQGRALDPDKYFIVVVNQIGSGLSTSPHNTPP
                     PAGMGNFPPVRIGDDVRAQHKFVTEKFDLKSLALVVGGSMGAQQTYEWAVRYPDMVKR
                     AAPIAGTARNTIHDSLFTETLVEAITSDPGFNNGFYASSADVREGLLRHAKMWAVMGW
                     STEFFQQDRHKALGFSSMSDFIINFMNGYFSVMDPNDLLCMAWKWQRGDVSRHAGGDL
                     RAALGRIKAKTFVMPMSSDMFFPPSDCQAEWRLIPDAEFRPIQTIDGHLALFGADPAA
                     IGQLDKHLSELLATEV"
     gene            complement(12040..13656)
                     /locus_tag="VN968_11420"
     CDS             complement(12040..13656)
                     /locus_tag="VN968_11420"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL29882.1"
                     /translation="MASATQNFDAVVIGAGFSGLHMLKSLRDKLGLKVLVYEAGGTVG
                     GTWYWNRYPGARCDSDAYIYCFTWDKQLMQEWEWSERYPEQPEILNYLEHVAKRHDLK
                     RDIQFNTSVTGAEFDERTNLWTVRTDTGEEVTARYLIAAVGTLSTTNMPQLKGLEKFK
                     GKWYHTSRYPHTGVDFTGKRVGVVGTGATAVQAIPEIAQQAKQLTVFQRTANYCVPAR
                     NGKVDPEVVKARKADYDGVAKRIRESFFGQEHYFMPKSALEATAEEREREFDRMWDAG
                     GFAFWLANYQDMFFSQQANDLCADYIKRKIRKTVKDPVVAEKLIPKGYAYGTKRQPLD
                     TNYYETFNKDNVRLVDAKTDGGIEEITEKGIRAGDKEYPLDIIVFATGFDAMTGPLKA
                     LNLKGRGGRTLDREWVDGPHTYLGISVAGFPNLFTITGPQSPSVLSNMPVSIEQHVEW
                     ITDCIETMRRTGKTTIEATPAAQHQWVAHVNEIVNATLMTSANSWYMSANIPGKPRAF
                     LPYLGPEGVGGYRKKCDEIAAKGYEGFELA"
     gene            complement(14296..>14955)
                     /locus_tag="VN968_11425"
     CDS             complement(14296..>14955)
                     /locus_tag="VN968_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HXL29883.1"
                     /translation="GKPGVDDRQARLIKNMYAQSDLASSVSEGFRVRDDVYKSISEEM
                     TAANRGAVSPRGFELSARRIGRLMREQFNLGFVDVGGWDTHVNQGAATGYLADRLGEL
                     GRALAGFSEEIGPAGWRDTVVVVISEFGRTFRENGDRGTDHGHGSVYWVLGGGINGGR
                     LLGEQVKIEQANLFQNRDYPVLTDYRAMFAGLFQRMFGLEPASVQRIFAGVHPAELGL
                     V"
BASE COUNT         2825 a         4620 c         4485 g         3025 t
ORIGIN      
        1 aaggagatcg tggaaaaccc ggaggtgatc cgcgcctatc tcggctccta cgtccacccg
       61 ccgacaccgg gagagaccta gtgctcaagc tatccggcgt aagcgcgagc tacggctcgg
      121 tgcctgccat cggcaatgtc aacatcgaga tcggcgaagg cgaggcggtc ggcctgttgg
      181 gcgcgaacgg cgccggcaag agcacctcct tgcgcgtgat ctcgggcctc gtgaagctca
      241 catcgggcac catcaccttt gccggcagca atctcgcgtc gctgccgccg cacaagattc
      301 cggaattggg gattgcccat gtgccggagg ggcggcaggt gtttccggaa atgacggtgc
      361 aggagaatct cgagatcggc gcttacgtgc cgaaggccaa ggcggagcgc agccgcacgc
      421 tggagctggt ctacagcatc tttccaagac tggccgatcg caagaagcaa ctcgccggca
      481 ccatgagcgg cggcgagcag cagatgctgg cggtcggccg cgggttgatg ctgaaaccgc
      541 ggctgctgat gctcgacgaa ccctcgctag gcctggcccc cgtcatgacc gacgtgacgt
      601 ttgaaaagat cggcgaaatc cacaagatgg gcaccgccat tctgctggtg gagcagaacg
      661 tcagccgcgc gctgtcgctg gtgcagcgcg cctatgtgct tgaaagcggc aacgtgatca
      721 tgcacggcac cagcgcggaa ttggctggca acaagcaggt gcaggcggcg tacctcggga
      781 tttgatcgag tcgagagtct ggatcgttcg gttgcagatg ctcggcatca cctctcccct
      841 tgtgggagag ctcggatcgc atcgcagatg cgatccgggt gagaggttac ggcctaacga
      901 tagatctgaa cccctcaacc ctcttccaca aggggagagg gagtacgctg ccgttgccga
      961 aatatcgggc ctttaatcca gcgtgatcat cgatagcgct gaagataagc gatgctctgc
     1021 gcgatgcttc gcattggatc cgacgaataa tcctgttcga caaaaaaatg gcgcactccg
     1081 gcagcgatcg aagcggcaat gatttcatcg aaatccagtg tcccattgcc aacttcggtg
     1141 aaggacccat ctgtcgccat atccttcaga tgcaccagac gaaatcgcga ttgatacgcc
     1201 ttcaaataag caacaggatc gacattgccc ttttttgccc aatagacatc cagttcgaat
     1261 ttcacaaatc ggtcattcgt attagccaac aagaaatcat aggggctgac tccattgggt
     1321 agactagaga attcaaaatt gtgattgtga taggcaaatg ttaatcccga atgcgtcgcc
     1381 agttcaccta tggagttcag gttttcgcaa acgcgcttcc aatcatcgat cgtttgtcgc
     1441 tccgactggt ccaccgatgg gcaaaccaca aacttcgccg ccaatgccgt tgccgtctcg
     1501 aatgatgcct cggtattttt gcgcaggctg ttgtagtcga tgtgcaacga tggggcgctg
     1561 agacccaatt ccccgagcag gtttcttgtc tgtttcaggt cgggaccaag gacgccggcg
     1621 aactcgactt cctgatagcc aagtcgggca atggctcgca aggtaccttc aaaatccgtg
     1681 ctcagcaggt cgcgaacggt gtacagttga agcccgattt cctggagata tccaccgtgc
     1741 gctgcggctg ctttcgccgc agcggcagca gcaagagctg tcaagacatg gcgtctggtt
     1801 ggtgtcatct gtgactctcc cgtgccgaaa gcccctaaag catgcatgat atcctgcttt
     1861 ttgcgccctg actctcggta atcattggaa cccgctcatc aagtcgccgc ccgaccaacg
     1921 cagcgtgccg gacccaaggc gtcacgttga ttcccggatg tcacttcgct cacccgggct
     1981 actatgttca ccgccgtcat tgcgaggagc gaagcgacga agcaatccag ctttcgcttc
     2041 gccgagccgc gctatggatt gcttcgctgc gctcgcaatg acagttagct caccaccacc
     2101 cgcctcggct cgacgatctc gaacatgcgc gggaattcgt ccatcagcgc catcaactcg
     2161 ccgatccgca tcggatcgcc ggtgaatttg attttgccgc ttgcaacggc ttccggaaac
     2221 gtcgtctgct ttgcgatgac ttcatcgagc acagcgcgcg ccagggtaaa actggcgtcc
     2281 gccgtggccg cctgcgcgcc cgcggtaaac gtcagcgcac agttctcgag gttgagaaca
     2341 aacgtctcgc gggtatcggt aaaacgccag ttgagcacga tgtgcttgcc ttcggccttg
     2401 gggccgttga gccggacgcc gagcacatcc cacagctgct cggtgcgcaa cgcggccagc
     2461 gtttcccgcg gcatcaccgc gcgcggctgg gacttcggca tgccctgccg caactcctgc
     2521 gcgccgaaca gataggcgtt gcgccaggtc gcgctctcgg cggcgtagcc aagctgctcg
     2581 aagctatcgg cgagcagcgc gcgggcgggt tgattgtcgg gatcggcgaa taccagatgg
     2641 ctcacggcct gcgccacgaa gcgaaactca cccttggcaa aatccccggt ggcacgcgcc
     2701 aggatcgcct ccgcgccgcc catatattcg acgtatttct tgccggactc gaccggcggc
     2761 agcggatcga gatggacggg attggcgtcg taccagccga gatatttctg gtagatcgct
     2821 ttcacgttat gccggatgtg gccgtaatag ccgcgtgcgt gccacgcgcc gtcgaggctc
     2881 gccggcagtt ttatcgtttc cgcgatttcg gtggccgtga gtccgtgatt catcaggcgg
     2941 atggtctggt catgggcgaa tttgtagaga tcgcgctgca ggcggatcat ggtatcgatg
     3001 cgctcgtgac cccagaccgg ccagtggtgc tggccgcaca tcgcatccgc tttgccgccc
     3061 cacatctgca gcgcctcgcc gagatatttc gaccaggcca gcgcgtcgcg cacgtcggcg
     3121 ccgcggaacg gcagcagatt atggaaattg tgggtgcagt tttccgcgag attcaagagc
     3181 ttgtagcgcg gaacaaaaaa atgcatttcc gccggcgctt cgctgttcgg cgccatctgg
     3241 aattcgaatt cgaggccgtc gaaagtgcgc ctgttgccgg tcgccatgat gaggtcggtc
     3301 gggcgcaaca gcgccaccga gcccgccgcc atcgacttgc ctagtccgca atcgacctgt
     3361 ccgcgcacac ccttggccag gaagggtccg aactgatatt gcgcgcgccg cagcatcgca
     3421 ggccctgcaa tgatgttttc cgaaaccgca tgctccatga acagattggg cgcgatgatc
     3481 gggatgttgc cgcccgccag cgccgcgtca tcgagcacgc cgcgcgcgcc gccccaatgg
     3541 tcggtatggg tgtgggtgaa gatgaccgcg gccacggtac gctttccgcg atgctggaaa
     3601 tagagctcca tggcggcgcg cgcgccttca atcgaggtca gcgtatcgac cacgatcacg
     3661 ccgcgctcgc cctcgatcag cgtcatgttg gcgatatcga ggcccctgac ctgatagacg
     3721 ccgggcacga cctcgaagag tccatggttc atgttgagac gcgactgccg ccacaggctc
     3781 gaccgtcggc ggagcttttt cttcggaaag aaagccgtat ggctcgaggc tccagaccac
     3841 cctgccctgc gccgacgtga tccgcgcatt ttccagcgtg ccgagaaaac cgcgcgacgc
     3901 ctcctcgaaa tcgcgtgtgt ccgagaacgg caacgccttc agcgtcgccg cgtgctgcgc
     3961 gaccaccgat gccgaagcgt ccttgggtac ttcgctgctg cctgtttggg tcattgagtt
     4021 gttctcccct gcatggtgcg aaagggcgga ttatgatgag ctgacgtcga tcgtcaaagc
     4081 cttcggcacg acggcgcaat agccgtaggt catctcgtca ttgcgagaag caacgcgaag
     4141 aaacaatcca gaagcccgtc attccgggat ggtccgaagg accagacccg gaatctcgag
     4201 attccgggtt cgatgcttcg catcgctccg gaacgacgac ctgctctcac cggccggatc
     4261 atcaggcgcg acatcaaccc cgtcggcagc tatcacttct tgaccttgct cgcgtccatc
     4321 ttgatcgatg ggtcgagcag ttttgtcgta aacgcggttt ccacggcgaa cggcttctcc
     4381 atgccgagat aggtctgcac cagttcgtag tccttcttca tccgcgcggc gtcgatccag
     4441 cccagcgcct tggtcgtggt aaaatcgtcg gtcatcagga acttgatgcg ctcccactgg
     4501 cgctcctggt tttccttgtc cagaccggac acctggtcga gcagcgcctt caggcagggg
     4561 ctggcgtcgg cgacgcaggc cgcaaaagcc ttctggctga tttttacaaa atcctcggcc
     4621 agtttcggat ttttctcgag gaaggcgccg ttgacgatca gcgaattgcc gtagggatta
     4681 agaccgatgt ccttccaatt gacaaacccg aggtcgctgc cgaactcgat gaccttcaaa
     4741 tcatgctcgt tgtagaaatc gctgatgata tcgacggtgt gacttttaag cgccgcgatc
     4801 ttggcggtcg gtccgacgtt gacaaagccg accgcgtccg gtgcgatccc cgccgccttg
     4861 gcgaacgccg gccacatcac gcgcgaggcg tcgcccggcg gattgccgat cttgtggccg
     4921 ggaaactctt ttgcgccgtt gacgccatag cttttcagcc aatagaaggt ctggccggta
     4981 ttggcgtaga tgctcatcag cgccacgaca tcggcgccct tgccgcgcgc aaccagcatg
     5041 gtggcgagat cggcgatgcc gagcggcgag ccgccggagc ccaccttcag cgacgaaacg
     5101 ccggaaccct tgccgacctc gatgttaaga tcgatgccgg ccttctcgta ccagccctgc
     5161 gccttggcgt aataaaacgg cgaatggtcg gcggtcggcg tccagttcag aatgagattg
     5221 acgggttcgg cggcacccgc aggcaacgac ggcgccagca atgcgagcgc tagtccagcg
     5281 atcccaaaag ctttgatccc aaaagccttc atggcaccct cctcgtttgc gtcgtttgcc
     5341 ggccccggcc agactttccg acggcacttg tcgttaccgg ccgttgcgtc taccaatgca
     5401 acaagggacg gaccgaattg tcaactgtct ggttggaaat gcccaaacaa cgatcaccca
     5461 aacgacgatc aaatggtaac aagccgcagc ggcgcgatcc ggtcgccacc cgcaaaaagc
     5521 tgctgacggc ggcgcgccgg gaatttgcac aaagcggcct cgccggcgcc agggtcgacg
     5581 aaatcgcgga tcgagccggc gtcaacaagc aactggtcta tcattatttc ggtgacaagg
     5641 acgcgctcta ccttgccgta ctcgaatggg tgtacgagga aatccgggcg caggagcgca
     5701 agttgaacct ggagggcctg cctcccgaac gggcgatcaa gaagctgatc gagagttcct
     5761 tcgaccacct cgcggcgcat cccgatttca tcgtgctgct caacgatgaa aatcgcggcg
     5821 gcgcccgcca tgtccgcggc tccagaaaga tcgagaccat gcattcgccc ctggtcagca
     5881 tggtctcgaa gattctgaag cagggcgttc gggctggatc cttccgcaaa ggcatcaacc
     5941 cggtgcatct ctacatttcg atcgcgggtc tcagctactt cttcttctcg aacacgccaa
     6001 ccttgtcggc gatcttcggc aaggacctcg ccagtccggc ggcgaagcgg acccgccgca
     6061 aacacgtcgt cgacctggtg atgcagtcgc ttcggcctta gcgacagccg tcattgcgag
     6121 gagcgaagcg acgaagcaat ccagcggcgc ctgggccatt ctctggattg cttcgcggag
     6181 cctgtcatcg ggcgcgcatt cgcgcgaccc gttggctcgc aatgacgatc tgcagcaata
     6241 atcaaccata tggttgaaaa attgcgggga tcgcgctagg ctgatctcgt ctgcggcgct
     6301 cgcgatacgc cggagcgcaa gaggagaaca tggtgtccag cgacggaatc cgttccgccc
     6361 gatcgtttgc gatcatcctg atcgtgcacc tggtcgtgat cgtgctgtgg caggttctgg
     6421 tcgacgcttt ccatgtgccg aaattcgtgc tgccctcgcc gctggccacc gtcgcgacgc
     6481 tgacatcggc aaaatatgca tggctttcca acctcgccgt gacggcggcc gaaatcctcg
     6541 gcggctttgc gcttggtgcg ctcgtcggcg tggtgctggc ggttctattt tgctggtcgc
     6601 cgctggtgag ccttttgttg ctgcccttgt tcgtcacgct gaacatgatt ccgaaagtgg
     6661 cgctcggccc gctgttcatc gtctggttca gctacgggat ttttccgaat atcctgatcg
     6721 ccttcagcat ctgcttcttc ccgatcctgc tgaccaccgc gcgcggactg agcgaggtgg
     6781 agccggatct cctggatctc gtgaaatcgc tgcgcggctc gcgctggacc ttgtttcgca
     6841 agatccagtt gccgggcgcg ctgccttacg tgttttccgg catgaaggtc ggcgccattc
     6901 tggcggtcgc gggcgccatc gtcggcgagt tcatcgcgtc cgaacgcggg cttggttatc
     6961 tgatgatcca ggtgcagtcc tcgctcgaca cgcccgcgat ggtcatggcg gtcgtgttgt
     7021 tgaccttgct gggggttgca ctttacggcc tggtgcttgg cctcgaacgg ctgttcgtgg
     7081 tcagggacgt gcggctgcaa taaggtcggt caggaaagac acatgctgct gacgcgcgaa
     7141 aatctgccgg acacgattcc cgatatcgcg gccctcgacg atctgttgtg ccggccgagc
     7201 caggcgctga tcgacgatct ccaaaaggtc gatggcgaca tcatgatcct cggcgtcgcc
     7261 ggcaagatgg ggccgacgct cgccggcctc gccaaggccg ccctgcccca tcgacgcatc
     7321 atcggcgtgg cgcgcttttc cgacgccagc gccaaggcgt ggctggaggc gcgcggcgtt
     7381 gaaaccctga attgcgatct gctcgatgaa gccgccatca aggcgctgcc aaaagtcaaa
     7441 aacatcgtct tcatggccgg acgcaaattt ggcgcggaag gcgacctgtc gttgacctgg
     7501 gcgatgaacg cccatgtccc ggccctggtc gcgcaggctt ttgtgcaatc gcggatcgtg
     7561 gcgttctcga ccggctgcgt ctatccgttc gtcccagtga acggaaacgg cgccgatgaa
     7621 actctcgcac ctaatccacc cggcgaatac gcgcaatcct gcgtcgggcg cgagcgcatg
     7681 ttcgaatatt tctcgcgccg gttttcaacg cccggccgct tgttccgcct gaactacgcg
     7741 atcgacatgc gctacggcgt gctgcacgac atcgcgacca agatcctcgg gggcaaggcg
     7801 atcgacgtca gcctcggcca tgtcaatttc atctggcaag gcgatgcctc ggcgcaggcg
     7861 ctgcgctgcc tcgcgcattg cgacacgccg acctcgccga tcaacgtcag cggccatgaa
     7921 atcctcagcg tgcgcgatct cgcggcaata ctcggcgcgt ttctcggccg cgatcccatc
     7981 atcaccggga ccgaacaacc gacagcctgg ctcaccgata cctcgcaagc cgcaaaactg
     8041 ttcggtccgc cgatcgtcga taccgcgcgg ttgatcgcgt ggaccgcgga ctgggtcgcg
     8101 cgcgcgatgc cgagcctcgg caaacccacc aaatacgagg tgcgcgatgg ccggtactga
     8161 ccacatggcc atcgttcagc tcggtccgga cgacgcgctc gcagggcttg cgctatcaac
     8221 agaggcgcac tggaatcaga acgaggccga ctggcgtttt tttctgagcc aaggaaccgt
     8281 gttcggcgtg cgcgatcatg acggccacct cgtcgcgagc gcggcgctat tgccctacac
     8341 atccggcaat gcctggatca gcatggtgct ggtgaccgca ggctggcgcc gccgcggtct
     8401 tgccaccaga ctcgtcgatg cctgtatcga caccgcggcg aaacaaggtt ttacaagctg
     8461 gctcgacgcg acgccggacg gcgccacggt ttatgcgccg ctgggctttg cgccgacatt
     8521 tgaactgcgg cggttgcggc ttgagattct tgcggtgccg aaagccacaa cgcctccatc
     8581 gctgtcgagc tacagtctcg atgaattcat aacgcgcgat atcagcacca tgggattcaa
     8641 tcgcagcgct ctgctggccg cactcggcgg acggccgggc tcgcggctgg tgtcgagcag
     8701 cagcgcggcg gcacttgtcc gcgatggccg caaggcccgg cacatcggtc ccctgtttgc
     8761 cgatagtccg gatcgcgcgc tcgcgctggt ccatgagatt atccgatccg aaaccgggcc
     8821 tatattgatc gacgccgtcg gcacgcaaga cgaatttctc gaggggctga ccagctccgg
     8881 ctggaccatc gagcggccgt ttcagcggat gcggttcggc ggcgccacgg ctcggcctac
     8941 ggagccgccg ttcgccgtcg ccggcccgga atatggataa ggagttgcac gatgcaccat
     9001 ggcgcgatga attcgaaggt gcggcgcctg attgccgatg gcacggtgat cccggcgcat
     9061 ccgctggcgc tcgacgccaa ccgcacgctc gacagcgtcc accagcgcgc gttgacgcgt
     9121 tactacatcg atgccggcgc cggcggcctc gcggtcggcg tccacaccac gcagttcgcc
     9181 attcgcgacg tcggcctcta ccggccggtg cttgaactgg ctgcggagac cgcggctaac
     9241 tggaccaggc ggccgctggc gctggtcgca ggtcttgcag gttcgacccg acaggccatc
     9301 gccgaggcgg agattgcgcg aggcatcggc tatcatgcgg gcctattaag cctcgcggcg
     9361 atgaagtccg cgtccgaaga cgagatcatc acccattgcg aggcggtggc gcgcgaaatt
     9421 ccgctggtcg gtttctatct gcagccggcg gtcggcggcg tcatcctcag cgcgaacttc
     9481 tggcggcgct ttgcggcaat cgacaacgtc atcgccatca agatcgcgcc gttcaaccgc
     9541 taccgcacgc tcgatgtcct gcgcggcgtt tgcgctgccg gcgcgctcga ccggatcgcg
     9601 ctctataccg gcaatgacga tcacatcctt ctcgaccttg cgctgccgtt cgatctgcgc
     9661 gatcggggcg tcaccgtcag aacctatttc cgcggcggcc tgctcggcca ctggtcggtg
     9721 tggaccgaaa ccgccatcaa gcaattcgag atgtgccgcg ccgcgcgcca caaggacacg
     9781 gtgccttcag atctcttggc gctcgatgcc cgcgtcaccg actgcaacag cgcgttcttc
     9841 gacgtcgcca acaattttca cggctgcatc gccggctgtc atgaaattct gcggcggcag
     9901 ggattgctcg agggtatctg gtgcctcgat cccgccgagg gcctcagccc cggccagatc
     9961 caggagatcg accgcgtctg caacgagcat gcggatctgg ccgacgacga tttcgtcgcc
    10021 gaccacttgc agaaatggct ggcatgacgg aagcgccctt cattcgtctg cgcaacgtcc
    10081 gaaaagtcta ccgttcgcaa ggcgtggaat ttctcgcggt ctccgacgtc accatggatg
    10141 tgcgggaggg cgagctgatc tcgctggtcg gcccaagcgg ctgcggcaag accaccgtct
    10201 tgaagatcct cgccgggctg cacggcgccg atggcggcac cattcaaatc ggcaacgcgc
    10261 gcacgccgtt cgaccccgcg cgcgacatcg gcatggtgtt ccagcaggcg ctgttgttga
    10321 aatggcgaac cgtgctcgac aacgtgttgt tgccggcgga gatcgtcggc ttgccgatca
    10381 aggcggcgcg cgcccgggcg cgcgatctgc tcaatctggt cgggcttgcc ggctatgagg
    10441 aaaaatatcc gcagcaattg tccggcggca tgcagcagcg cacggcgatt gcgcgcgcct
    10501 tcatccacga tccaaagctg atcctgatgg atgagccgtt cggcgcgctc gatgcgctga
    10561 cccgcgaaca gatgaatctc gaaatgctgc ggatctggcg cgaaagcggc aagaccatca
    10621 tctttgtgac gcacagcatc catgaggcgg tgttcctcgg ctcgcactgc gccgtactca
    10681 ccgcgggacc cgcgcgaatg gcggaatatt tcccgatcga tctgccgttc ccgcgcgcgc
    10741 tgccggtcaa gaccaccgat gaattcggcg cctatgcacg gcggatctat tcgagcctgg
    10801 ggctcggccc gagtgcgtga acccatgaac gcgcccacgg ctgatgactg agtaccactg
    10861 gaagcagagg ccccgatggg tgcggcgccc ctgccaaatt tccatcgcga actaaacttc
    10921 tgtggcgagc agttcgctca ggtgcttatc gagctggcca attgcagcgg gatcagcacc
    10981 gaaaagcgca agatggccat cgatggtttg gatcggccgg aactcggcat cgggaatcag
    11041 ccgccactcg gcctggcaat cggacggcgg gaagaacatg tccgaagaca tcggcatgac
    11101 aaaggtcttg gctttgatgc ggcccaatgc ggcccgcaag tcgccacccg cgtggcggct
    11161 gacatcgcca cgctgccatt tccaggccat gcacagcaaa tcgttgggat ccatgacgct
    11221 gaaatagccg ttcatgaagt tgatgatgaa atcgctcatc gacgaaaagc ccaacgcctt
    11281 atggcggtct tgctggaaaa attccgtgct ccagcccatc acggcccaca ttttagcgtg
    11341 gcgtaacagg ccttccctga cgtcggccga cgatgcgtag aaaccgttgt tgaaaccggg
    11401 gtcggaggtg atcgcctcga ccagcgtttc cgtgaaaaga gaatcatgga tggtgttcct
    11461 ggcggtgccg gcgatgggcg cggcgcgctt caccatgtcg gggtaacgca ctgcccattc
    11521 ataggtctgc tgtgcgccca tcgagccgcc caccacgagc gcgagactct tcaagtcgaa
    11581 tttttccgtg acaaatttgt gttgcgcgcg cacgtcatcc ccgatgcgca caggggggaa
    11641 atttcccatc ccggccggcg gcggtgtatt gtgtggcgag gtggaaagcc cactgccaat
    11701 ctgattgacg acgacgatga aatacttgtc gggatcgagc gcgcggccct gaccgatata
    11761 tacctgctcg atgatcttgt ttgtgccgga gtaccaggtg gggatgagga tggcgttagt
    11821 tttcgcagca tttagtttgc cgaaagttgc gtaggcgaga ttgcaggcac gaatcgtgcc
    11881 gccttcctcc agctcgagat tgccgatgtc gtaaagctcg taaggtccgt gaacggcctg
    11941 cgagtagtag ctgttgtcga tcattgtcgc ctcccgattc cgtttgttgg gcggcaaggc
    12001 tgatgccttg ccgccctgtt ttttctcgtc caggtttggt tacgccaatt caaatccttc
    12061 gtagcccttg gccgcgatct cgtcgcactt cttcctgtaa ccgccgacgc cttccggccc
    12121 cagatagggc aagaaggcgc gcggcttgcc ggggatgttg gcgctcatgt accaggagtt
    12181 ggcgctggtc atcagcgtcg cgttgacgat ttcgttgaca tgggcgaccc attgatgctg
    12241 cgccgcgggc gttgcctcga tggtggtctt tccggtcctg cgcatggttt cgatgcagtc
    12301 ggtgatccac tcgacatgct gctcgatcga taccggcatg ttcgacagca ccgacgggct
    12361 ttgcgggccg gtgatggtga acaaattggg aaagccggcg accgagatgc caaggtaggt
    12421 gtgcgggccg tccacccatt cccgatccag cgtgcggccg ccgcgccctt tcaggttcag
    12481 ggccttcagc gggccggtca tggcatcgaa accggtggcg aaaacgatga tgtcgagagg
    12541 atattccttg tcaccggcgc ggatgccctt ttcggtgatt tcttcaatcc cgccgtcggt
    12601 cttcgcatcg accagccgta cgttgtcctt gttgaaggtc tcgtagtagt tggtgtccag
    12661 cggctggcgc ttggtgccgt aggcgtagcc cttgggaatc agcttttcgg cgacgaccgg
    12721 atctttcacg gtcttgcgga tcttgcgctt gatgtagtcg gcgcacaaat cattggcctg
    12781 ctgcgagaag aacatgtcct gatagttggc cagccaaaag gcgaatccgc cggcgtccca
    12841 catcctgtcg aactcacgct cgcgctcctc ggctgtggcc tccagcgccg acttcggcat
    12901 gaagtaatgc tcctggccga agaacgattc ccggatccgc ttggccacgc cgtcatagtc
    12961 ggcctttcgc gccttgacca cctcggggtc caccttgcca ttgcgcgccg gcacgcagta
    13021 gttggcggtg cgctggaaca cggtgagttg tttggcctgc tgcgcgattt cgggaatggc
    13081 ctgcaccgcc gtggcgccgg tgccgaccac gcccacgcgc ttgccagtga agtcaacgcc
    13141 agtgtgaggg tagcggctgg tgtggtacca tttgcccttg aatttctcca aacccttcaa
    13201 ctgaggcatg ttcgtggtcg acaacgtacc cacggccgcg atgaggtaac gtgcggtaac
    13261 ctcttccccc gtgtcggtac gcaccgtcca cagattggtt ctctcgtcga attcggcgcc
    13321 tgtcacgctg gtattgaatt gaatgtcgcg cttgaggtcg tggcgtttgg caacatgctc
    13381 aagatagttg aggatttccg gctgctcggg atagcgttcg gaccattccc actcctgcat
    13441 gagctgcttg tcccaggtga agcaatagat gtaggcgtcg gaatcgcaac gcgcaccggg
    13501 gtagcggttc cagtaccagg tgccgcccac ggttccgccg gcttcgtaga ccagcacctt
    13561 caaccccaat ttgtcccgta gcgacttcag catgtgcagg ccggaaaagc ctgcgccgat
    13621 cacgacagcg tcgaaatttt gagttgcgct agccatttgt ccctccagtt ttgttgttta
    13681 aatcgacgac cgagaaacgc gatcctccgc gaatgggaaa aactcgggca gctacgccgc
    13741 cttccacacc gcatcggccg cggcatggac gccgctttcg cgagctgcga acatttattt
    13801 gtccgcgaac gtcagcacct gcatcaggat gacacctgaa atttcacgcg aggggagcga
    13861 tccggtttat ggttggcgag gcctgctcga gcgaggccgc cgaggctgcg gcaacgcctg
    13921 gtcgatctga atcttgcttt gcatgtcgca cctcccgttt ttgatttttt aagacggccg
    13981 atcttcctcc cgaacaagat ttcctgcaag aacaagaaat cgaccgcgcg tctgttcggg
    14041 caaagcgtca aaagccgatg cgattgcaac gggcattgat ccggctgagg cgtgaggccg
    14101 acggaagttt ggtgcgacag caaagctgtc ccatgttttg caccgcacga ttccagaatc
    14161 gttgagttga aggaagccct tctgaccccg cttcaaaaaa ggcagaagct cggctggcca
    14221 tgaaatcgga gcaagcaccg cgcgcacagc ggatgtatgc aagcccgggc ttggttcctg
    14281 cgcgtagccc gctgcctaaa ccaatcccag ctccgccgga tggacgcccg caaaaatccg
    14341 ctgaacgctc gcgggttcca gcccgaacat gcgctggaac agcccggcaa acatggcacg
    14401 atagtcggtc agaaccggat agtcgcggtt ttgaaaaagg tttgcctgct cgattttgac
    14461 ttgttcgccg agaagacggc cgccattgat gccgccgccc aatacccagt agacgctgcc
    14521 atggccgtga tcggtgccgc ggtcgccgtt ctcccgaaag gtgcggccaa attcggagat
    14581 cacgaccacc accgtatcgc gccatcccgc aggtccaatc tcttcggaaa aacctgccag
    14641 cgcacgtccg agttcgccga ggcggtcggc gagatatccc gtcgccgccc cctgattgac
    14701 atgggtgtcc cagccgccga catcgacaaa accgaggttg aactgctcgc gcatcaagcg
    14761 gccgatacgc cgcgccgaca gctcgaagcc gcgcggcgaa accgcgccgc gattggccgc
    14821 ggtcatctct tcggatatcg atttgtagac atcgtcccga accctgaatc cctccgaaac
    14881 cgaagaagca agatcgcttt gcgcgtacat gttcttgatc agcctggcct ggcgatcgtc
    14941 gacgcccggc ttgcc
//
LOCUS       DATNOI010000293         7738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1076060,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000293 DATNOI010000000
VERSION     DATNOI010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7738
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076060"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..1246
                     /locus_tag="VN968_11430"
     CDS             5..1246
                     /locus_tag="VN968_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HXL29884.1"
                     /translation="MTSAMFDFAPLLPAGLPAPAARWTGLAKYSFVGGNNDPDQVPVE
                     GLVDAVNAVLKREGKTLATYGLASGPQGYRPLREFITTKLKRDAGIGCVADDILIVSG
                     SLQALDLVNATLLARGDTVITERDTYQGALNRLTRLGVNPVGIPLDHDGMRMDALAAA
                     LEDLKRRGITPKYIYTIPTVQNPTGTIMPAARRTELLKLAGQYGVPIFEDDCYADLIW
                     DGQRPPALYSMSTNAGVIHIGSFSKSIAPALRVGYVVAPWAMLSRMLALKTDAGSGAL
                     EQMVLAEYCAPHFASHVPKLTRGLRAKLDTLMAALNEQFGTSAEFEDPKGGIFLWVKL
                     PDNVDTLKLYQSALAAGVAINPGPEWSTDEAYAGCRLRLCFANPSHEQIREGVAVLAE
                     VCAKEFGVPARIANVEKQVRA"
     gene            1321..2223
                     /locus_tag="VN968_11435"
     CDS             1321..2223
                     /locus_tag="VN968_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXL29885.1"
                     /translation="MTETAAQRRIGIALVIAAAVAWSTAPFFTRLLPFDSWTILFWRG
                     LFGGGLIAAVLVLTQGRTGLRDLAMMGKSGWLVASLSTLGMVCFIPALQLTSVSNVAV
                     IIATGPFVAAALAWIWLREAARLRTLLASLVAFCGVVIIVSDAQASSDIFGIGLACLM
                     TLAIAAMTVAVRRHKNTPMVAAAALSNVLGSLVSIPFAQSITTVTGADVFIFAMFGFF
                     QVALGLTLFFLGSRLLPSGQASLIATLETPLMPFWVWLAFQEIPALRALVGGALVIGA
                     VMADIIGDARAQRQIAGASNHPPT"
     gene            complement(2246..2626)
                     /locus_tag="VN968_11440"
     CDS             complement(2246..2626)
                     /locus_tag="VN968_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29886.1"
                     /translation="MAPGIVLIGDAAGHNDPSGGQGISIALKDARLVCEALDATKAWT
                     PETFVPYASQRREQMRRLRFSSRLLSTYRMEFTDQARQRRRMGRKRMAADPELALPFM
                     ALQKGPFAVPSEAFSKRIWDRLLD"
     gene            complement(2605..3483)
                     /locus_tag="VN968_11445"
     CDS             complement(2605..3483)
                     /locus_tag="VN968_11445"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXL29887.1"
                     /translation="MRPSDSKPDVVIVGAGIAGSALAISLAGSGHGVTLLEKSTVHVD
                     RVRGEFIVPWGVAEAKTLGILDVLESAGGNYTVRSIPYGEELSPEDARKYSVPMDQMV
                     PGVKGALNLGHPRICDALNEAALAAGATLLRGVDRIQVSAGHPPRIEFAHSGLNHALS
                     PRIVIGADGRGSTVARQVGFERLADPIHHILSGLLVEEAHEWPLDEQSIGVDGDVGFY
                     IFPQGNGRVRLYATHGLVQRNRFEIALPKDSCRPLTGRRFRTAVRFREAGQPVPAMAI
                     QTMTSGSTIPWLPVSS"
     gene            complement(3647..4690)
                     /locus_tag="VN968_11450"
     CDS             complement(3647..4690)
                     /locus_tag="VN968_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HXL29888.1"
                     /translation="MSSASAATFVYVGNAESQDVTVLELKSSGDLTPVETAGVPGPAK
                     PGSSLPLAVSPDKTRLYVGLRNEPYSAVTFAIDARTGKLTLLGSGPLADSMAYVVTDR
                     SAKFLLGASYGGNKVTVNPIGPNGVVQPAREIIATQPNAHCIVPDPSNRYVLHTSLGG
                     DLVYQEKFDVKTGKLTPNDPPSVSIKAKAGARHLVFSPNRKFVYLVNELDASIYAFPW
                     DAKTGTLKKEVQVATALPKGFEGKPWAADIHLTPDGRFLYASERTTSTLAAFSVDLKT
                     GLLTSIDSYPTEKQPRGFNIDPTGRYLLSVGQLSNSMTSYAIDKATGKLTRLKEYPVG
                     KNPNWVEIVSFSR"
     gene            complement(4815..6107)
                     /locus_tag="VN968_11455"
     CDS             complement(4815..6107)
                     /locus_tag="VN968_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HXL29889.1"
                     /translation="MSALSRRNFLGLTGSAAAAALSGRFATQAEAAMGSADKFDLVIK
                     GGEVLDPSQSLRGKRDIGIRWGVVEAIEAEIPAARASKTIDASGKLVTPGLIDLHCHV
                     YPYGSAIGIPADELVQFQGTTTVVSAGDAGVNNLAALRRYIVAQSRARIYAFVHIANN
                     GLSAFPVAELYNIDNAQVEACAMALAENPDFLIGVKVRMSENVIFKHGLEPLKRAIQA
                     CEMSGWPAKMMVHIGGVETAGLMSNILDLLRPGDVLTHAYSGAPNMSGAFTNIVQDGK
                     LLPAALAAKQRGVIFDVGHGGGSFDFTVAEVAIPAGCAPDTISSDIHVFSGNSPGVPF
                     LPNVMSKFMAMGFSLEQVVTMTTTAPAKIINRAPRIGTLQIGAPGDVAIMDLVEGPVT
                     FLDTRNNKRDGKAYLKPVQTVINGVPFGRPYQSPFAVR"
     gene            6371..6718
                     /locus_tag="VN968_11460"
     CDS             6371..6718
                     /locus_tag="VN968_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29890.1"
                     /translation="MIGSWSLDCSVAPDRGKGAVLAYEIAPGDRVIHRRDFGDTTDES
                     EVITAEVSRNGMLNLRVFFPKLKQIREYGFVLEPDGALRAVYNRSQQGQYTIRNGKFT
                     ANGNPTLALHKCM"
     gene            6880..7113
                     /locus_tag="VN968_11465"
     CDS             6880..7113
                     /locus_tag="VN968_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29891.1"
                     /translation="MKKLAYVIAALGAIAIAAPSIASAETVVIRHGGDRDHHRVYGAR
                     AEFREHRDHGWHEGWRHRHADRVVVVKHRHHEY"
     gene            7283..>7738
                     /locus_tag="VN968_11470"
     CDS             7283..>7738
                     /locus_tag="VN968_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29892.1"
                     /translation="MIKLILAPIRSMVRFPLLQFAVVIGVILWLQAADDRSIFGQIFG
                     ALDKLVDATVQLFSAVFTVKSFTKSWLISGFMIAYVYLAGSLLLFLVRLLINAAIDLV
                     GRANLLYLRGAIARERGIKAYRAWVPLERIRPANVSQREWEECFAWPADN"
BASE COUNT         1444 a         2398 c         2345 g         1551 t
ORIGIN      
        1 gttcatgacc tccgcaatgt tcgacttcgc acccctgctg ccggcgggat tgcccgcgcc
       61 cgcggccagg tggacgggac ttgcaaaata cagttttgtc ggcggcaaca acgaccccga
      121 ccaggttccg gtcgagggcc tagtcgacgc ggtcaacgcc gttctcaagc gcgagggcaa
      181 gacgctcgcc acttacggcc tcgccagcgg cccgcagggc taccgtccct tgcgcgaatt
      241 catcaccaca aagctcaagc gcgatgccgg gatcggctgc gtcgccgacg acatcctgat
      301 cgtgtcgggc tcgctgcagg cgctcgacct cgtcaacgcg acgctgctgg cgcgcggcga
      361 taccgtcatc accgagcggg acacctacca gggcgcgctg aaccgtctga cccggcttgg
      421 cgtcaacccg gtcggcattc cgctcgacca tgacggcatg cggatggacg cgctggcggc
      481 ggcgctggag gacctcaaac gccgcggcat cacgccgaaa tatatctata ccatcccgac
      541 cgtgcagaat ccgaccggca ccatcatgcc ggcggcgcgc cgcaccgaac tgttgaagct
      601 cgcaggacaa tatggcgtgc cgatcttcga ggacgattgc tatgccgacc tgatctggga
      661 cgggcaacgc ccgccggcgc tgtattccat gagcacaaat gccggcgtca tccacatcgg
      721 ctcattctca aaatcgatcg cgccggcgct gcgggtcggc tacgtcgtcg caccctgggc
      781 catgttgtcg cggatgctgg cgctgaagac cgacgccggc tccggcgcgc tggagcagat
      841 ggtgctcgcc gaatactgcg cgccgcattt tgccagccac gtcccgaagc tgacgcgcgg
      901 cctgcgcgcc aagctcgaca cgctgatggc ggcgctcaac gagcagtttg gaacgtcggc
      961 ggaattcgaa gaccccaaag gcggcatctt cctgtgggtg aaactgcccg acaatgtcga
     1021 caccttaaag ctctaccagt cagcacttgc cgccggcgtc gccataaacc ccgggcccga
     1081 atggtccacc gacgaggcct atgccggctg ccggctgcgg ctgtgctttg cgaacccctc
     1141 ccatgagcag atccgcgagg gcgttgccgt gctggccgaa gtgtgcgcta aggaattcgg
     1201 cgtgcccgcc agaatcgcga atgtggagaa gcaggttcgc gcgtagatac caggcggttt
     1261 catcaaatcg gtgtcctgtc gccgcggggc agaacatgag ccggtcacag gattcctttt
     1321 atgacggaga ctgctgcaca acgtcggatc gggattgccc ttgtcatcgc cgccgccgtg
     1381 gcctggagta cggcgccatt cttcacccgg ctccttccct tcgattcgtg gacaatattg
     1441 ttctggcgag ggctgttcgg cggagggctc attgcggccg ttctggtact gacgcaaggc
     1501 cgaaccggcc tgcgggatct ggcaatgatg ggaaagagcg gctggctcgt cgcatccctg
     1561 tcaacgctgg gcatggtgtg tttcatcccg gccctgcagc tcaccagcgt gtcgaatgta
     1621 gcggtcatta tcgcaacggg accatttgtc gccgctgcgc tcgcgtggat atggcttcga
     1681 gaagccgccc ggttgcgaac cttgctggca agcctcgtgg cgttctgcgg cgtcgtcatc
     1741 attgtcagcg atgcgcaggc gagctccgac atcttcggga tcggcctggc ttgcctcatg
     1801 acccttgcga tcgcagccat gaccgtcgcc gtccgacgac acaagaacac cccgatggtg
     1861 gctgccgcgg cgttgtcgaa cgtgctgggc agccttgtca gcatcccctt cgcgcaatcc
     1921 atcaccaccg tgaccggagc cgatgttttc atttttgcga tgttcgggtt ctttcaggtg
     1981 gcgttgggtc tcactttgtt ttttctcggc tcacgcctgt tgccatccgg gcaggcatcc
     2041 ctgatcgcga cgctggagac accactgatg cccttttggg tgtggctggc gttccaggag
     2101 attcccgctc tgcgggcgct tgtcgggggc gccttggtca tcggggcggt catggccgac
     2161 atcatcggcg atgcccgcgc gcagcgacaa attgccggcg catcaaatca tccgccgacg
     2221 tgaaattcga cgttgccgcc gcgacctaat ccagcaagcg gtcccagatg cgtttcgaaa
     2281 aggcctcgct cggaacggca aaaggtccct tctgcagcgc catgaacgga agcgctaatt
     2341 ccggatcggc cgccatacgc ttgcgcccca tccgtcggcg ttgcctggct tggtcggtga
     2401 attccatccg ataggtcgaa agcagccttg agctgaaccg aagccgccgc atctgctcgc
     2461 gccgctgcga cgcatagggg acgaacgttt cgggagtcca ggctttggtc gcgtcgagcg
     2521 cttcgcagac cagccttgca tctttcaggg cgatgctgat cccctgcccg cccgacgggt
     2581 cgttgtggcc cgcggcgtcc ccgatcagga cgataccggg agccacggga ttgtcgatcc
     2641 agacgtcatt gtttggatag ccatggcagg gaccggctgg ccggcctcgc gaaagcgaac
     2701 cgccgtgcgg aatcgacggc cggtcaaagg cctgcaggaa tctttcggta gcgctatctc
     2761 gaagcggttg cgttgaacga gaccgtgagt ggcgtagagc cggacccttc cgttaccttg
     2821 aggaaaaata taaaatccaa cgtcgccatc gacgccgatc gactgttcgt cgagcggcca
     2881 ctcgtgcgct tcctccacca gaagcccgct caggatgtga tggatcgggt cggccagccg
     2941 ttcgaacccg acctggcggg cgaccgtaga gccgcggcca tcggctccaa tgacgatcct
     3001 tggtgaaagg gcgtggttca ggccggagtg tgcgaattca attcgaggag gatgcccggc
     3061 actgacttga atgcggtcga ccccgcgaag cagcgtggca ccggcggcga gcgcggcctc
     3121 gttaagggcg tcgcagatcc tgggatgccc gaggttcaag gcgcccttga cgccgggaac
     3181 catctgatcc atcggcaccg aatattttcg ggcgtcctcc ggcgaaagct cctcgccgta
     3241 tggaatcgac cggacggtgt agtttccgcc ggcgctctcc aggacatcca gaatcccgag
     3301 cgttttggct tccgccacgc cccacggcac aatgaattcg ccgcgaaccc ggtcgacatg
     3361 aactgtcgac ttctccagca atgtgacgcc gtggcctgaa ccggcaagcg aaatcgcgag
     3421 cgcgctgccc gctatgccgg ctccgacgat gacgacgtcg ggcttgctgt ccgaaggtct
     3481 cattggtttg cgtccggtat ttcacctcac cgccaacggc gattgatacg gcggcggcca
     3541 tgctagagcc ttttccgttc cgattgaatc ggaacggggg actctatttc ttgttttgac
     3601 gcgttttctt cacgcgaacc ggtaatccac ttcgcttgaa aacgctttag cgcgagaagc
     3661 tgacgatctc gacccagttc ggattcttgc ccaccggata ttccttgagc ctggtcagct
     3721 tgccggtcgc cttgtcgatc gcataactcg tcatgctgtt ggacaattgg ccgacagata
     3781 gcagatagcg gccggtcgga tcaatgttga agccgcgcgg ctgcttttcg gtcggatagg
     3841 aatcgatcga tgtcagcaat cccgtcttga gatcgacgct gaatgccgca agcgtgctgg
     3901 tggtgcgttc ggaggcgtag agaaatctgc catccggcgt gaggtggatg tcggccgccc
     3961 acggcttgcc ttcgaaccct ttcggcaagg cggtggcgac ctgaacttcc ttcttcaggg
     4021 taccggtttt ggcatcccac gggaaagcgt agatcgatgc gtccagttca ttgaccagat
     4081 agacgaactt cctgttcggc gaaaacacca gatgacgcgc acccgctttg gccttgatgc
     4141 tgacgctcgg cggatcgttc ggcgtcagct tgccggtctt gacgtcgaat ttttcctgat
     4201 acacaaggtc gccgccgaga ctggtgtgca gcacgtagcg gttcgatgga tcgggcacga
     4261 tgcaatgcgc attcggctgc gtcgcgatga tctctcgcgc cggctgcacg acgccgttcg
     4321 gtccgatcgg attgaccgtg accttgttgc cgccatacga cgcaccaagc aggaactttg
     4381 cgctccggtc ggtaacgaca taggccattg aatcggccag cggaccggag cccaacagcg
     4441 tcagcttgcc ggtcctggcg tcgatcgcga aggtcaccgc cgaatacggc tcgttgcgca
     4501 agccgacata aagacgcgtc ttgtcagggc taaccgcaag cggcagcgag ctaccgggct
     4561 tggcgggacc gggcacacca gccgtttcga ccggcgtcag atcgccgctc gatttgagtt
     4621 ccagaacggt gacatcctgg ctttcggcat ttccgacata gacaaaggtg gccgcgctcg
     4681 ccgatgacac gaaaaatcct gctcccaata aggccgtcac gcagaataat ggcttcagac
     4741 ttactcgcgt tcgcatggcg tctcccgacg atgtgttgat tgaacatggg ttggtgactt
     4801 ggtggaaacg ccggctatct caccgcgaac ggcgattgat acggccggcc gaacggcacg
     4861 ccgttgatca cagtctgcac cggcttcaaa taggccttgc cgtcgcgctt gttgttgcgg
     4921 gtatcgagga acgtgaccgg cccctccacg aggtccatga tcgcgacatc gccgggcgcg
     4981 ccgatctgca atgtgccgat cctcggcgcg cggttgatga ttttcgcggg tgccgtggtg
     5041 gtcatcgtca ccacctgctc cagcgaaaag cccatcgcca tgaacttgct catcacgttc
     5101 ggcaggaacg gcacgccagg cgaatttcca gagaacacat gaatgtcgga tgagatcgtg
     5161 tccggcgcgc agccggcggg gatcgccact tccgccacgg taaagtcaaa actgccgccg
     5221 ccatggccga catcgaagat cacgccgcgc tgcttggcgg cgagcgccgc cggcaacagc
     5281 ttgccgtcct gcacgatgtt ggtgaaagcg ccggacatgt tcggcgcgcc ggaataggcg
     5341 tgggtcagca cgtcgccggg ccgcagcagg tccagaatat tcgacatcaa cccggcggtc
     5401 tcgacgccgc cgatatgcac catcatcttg gccggccagc cggacatctc gcaggcctgg
     5461 atcgcccgct ttagcggttc caggccgtgc ttgaagatca cgttctccga catccgcact
     5521 ttcacaccga tcaggaaatc cggattttcc gccagcgcca tggcgcaggc ttcgacttgc
     5581 gcgttgtcga tgttgtagag ctcggcgacc gggaacgccg acaatccgtt gttggcgatg
     5641 tggacgaatg catagatccg cgcccgcgac tgcgccacga tgtagcggcg caaggccgcg
     5701 agattgttga caccggcatc gcccgccgac accaccgtgg tggtgccctg aaattgcacc
     5761 agctcgtcgg cgggaatacc gatcgccgag ccgtaggggt agacgtggca atgcaggtcg
     5821 atcagcccgg gcgttaccag tttgccggac gcgtcgatgg tttttgatgc acgcgccgcc
     5881 gggatttccg cctcgatcgc ttccaccacg ccccagcgaa tgccgatgtc gcgttttccc
     5941 cgcagcgact ggctgggatc gagcacctcg ccgcccttga tcacaaggtc gaacttgtcg
     6001 gcggagccca tcgcggcctc cgcctgtgtc gcgaagcgtc ccgacagtgc agcggccgca
     6061 gccgatccgg taaggccgag aaaattgcgg cgtgacaatg cggacatgct ggcgttcccc
     6121 ccgaatatgt tttcttatgc gaggatgatg cgccgggtgc cggctttccg caagccgccg
     6181 gcgaaccaga gccggcgcat ttgcgccgaa atccaaatgc taggctgcga tcgcggatca
     6241 tccacgaaaa gcgggggcgc cagacttgag tgggcctttt agtcgaccgg cgctggctat
     6301 ccttttctcc ctgacgttcg cgtccaacgc ttccgcggac acggtcgccg agacggcagg
     6361 cgcatgggga ttgattggat cctggtcgct ggactgttcg gttgctcccg acaggggcaa
     6421 aggcgctgtg cttgcctatg agattgctcc cggtgaccgg gtcatccacc ggcgcgactt
     6481 cggcgatacc accgatgaaa gcgaagtgat caccgccgag gtttcccgaa atggcatgct
     6541 gaatttgcgc gtcttcttcc ccaagctcaa acagatccgc gagtatggat ttgttctgga
     6601 gcccgacggc gccttgcgcg cggtatacaa tcgcagccag caaggccaat acaccatccg
     6661 gaacggcaaa ttcaccgcca acggcaaccc aactctggca ctgcataaat gcatgtagga
     6721 aacgccgggt tgagcccatg gaaggctgcc ctcaggaaag cgaatggatg cctggctggc
     6781 ctctccgtcg ctgaacagct gttcagaatt ctcttcgaat tcacgcggaa cgttcccgct
     6841 tgtgtgaggt ttaatcctcg cacaaccgag gaggacagca tgaagaaact tgcatatgtt
     6901 attgcggctc ttggcgcgat tgctatcgca gcgccgtcga tcgccagcgc tgaaaccgtt
     6961 gtgatcaggc acggcggcga ccgcgaccat catcgcgtct acggcgcacg cgccgagttc
     7021 cgcgaacatc gcgatcacgg ctggcacgaa ggctggcgtc atcgccacgc cgacagagtg
     7081 gtggtcgtca agcatcgtca ccacgaatac tgagcccgac acaggcacac cacgagaatg
     7141 gccccccgag gggccattct ttttttgcgc agactgccgt cagcgaaagg tagtagagtt
     7201 agtcagtcct gcgatagaga gacatcgcca tgaacttttg gccattcccc gtgaggaatg
     7261 ccactctggg agagcccgcc ggatgatcaa gctgattttg gctccaatac gaagcatggt
     7321 gagatttccc ctattgcagt tcgctgtcgt gattggcgta attctgtggc tgcaagccgc
     7381 cgacgatcga tcgatcttcg gtcaaatctt cggtgccctg gacaaactcg tcgacgctac
     7441 ggtgcaattg ttctcggccg tttttaccgt aaaatccttc accaaatcat ggctcatatc
     7501 gggcttcatg attgcgtatg tgtacctcgc cggctcattg cttctgttcc tggtgcgcct
     7561 cctcatcaac gccgcgattg atttggtcgg acgggccaac ttgctgtacc tgagaggcgc
     7621 cattgcgcga gaacgtggca tcaaggctta tcgcgcgtgg gtgccgctcg agagaatcag
     7681 accagcaaat gtctctcagc gagaatggga ggagtgcttt gcgtggcccg ccgacaac
//
LOCUS       DATNOI010000294         2128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_474820,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000294 DATNOI010000000
VERSION     DATNOI010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474820"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VN968_11475"
     CDS             <1..495
                     /locus_tag="VN968_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL29893.1"
                     /translation="YMSLAFYRAHPERVRALLIIDTGPGFKKDDAREAWNRRAHETGE
                     RFEREGLAVLKSASRERSDVSHRDASGLARAARGMLTQRDARVIELLPDIKVPSLIVV
                     GADDTPFLAASDYMAAKIPAAQKVVIPAAGHAVNIDQPQAFIDAVLPFLDGLDARTAQ
                     KAAS"
     gene            492..848
                     /locus_tag="VN968_11480"
     CDS             492..848
                     /locus_tag="VN968_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29894.1"
                     /translation="MKKLLAAALSMVAVSGGAIAPVNAEALPPIGGPYPPPFTATLSN
                     NIPLVFGMDAEDAARALGTPLNYVRGRPGDEIYLAFRNLGGSGLFPHRHRLYLQFRHG
                     RLAGWKGDWGHNWMWQ"
     gene            923..1597
                     /locus_tag="VN968_11485"
     CDS             923..1597
                     /locus_tag="VN968_11485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXL29895.1"
                     /translation="MGKDIKLTASDNFQLGGYRADPATPPKAAIVVIQEIFGVNHHIR
                     AVCDRLADEGYVAIAPSIFDRVERNFQCGYSPEEVAVARKFVANPDWAAILRDTQAAI
                     DAVKDVGPVGIIGFCLGGSVAYAAATKLSGLSAAIGYYGGAIVRFADDKPKVPTQLHF
                     GEKDAGIPLSDVETIRAKRPEVEIHVYPGAQHGFHCDERASYDKTSADIAWPRSLAFF
                     ARHLKK"
     gene            complement(1822..>2128)
                     /locus_tag="VN968_11490"
     CDS             complement(1822..>2128)
                     /locus_tag="VN968_11490"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HXL29896.1"
                     /translation="FWEGHDPTQGMRQGNDVGTQYRSGIYVTTPAQRKAAEESKAMYG
                     KALAADRHGAITTEILDAGEFYFAEDYHQQYLAKNPAGYCGLGGTGVSCPIGAGVSA"
BASE COUNT          402 a          696 c          638 g          392 t
ORIGIN      
        1 tacatgtcgc ttgcatttta tcgcgcgcat ccggaacggg tgcgcgcgct tttgatcatc
       61 gataccggac ccggcttcaa gaaagacgat gcccgcgaag cctggaacag gcgcgcacac
      121 gagaccggcg agcgcttcga gcgcgagggg cttgcggttc tgaaatccgc cagtcgcgaa
      181 cgctctgatg tgagccatcg cgatgcgtcg ggcctggctc gcgccgcgcg cggcatgctg
      241 acccagcgcg atgcccgcgt gatcgagctt ctgcccgaca tcaaggtgcc ttcgctgatc
      301 gtggtcggcg ccgatgacac gccgtttctc gcagcctccg actacatggc ggcgaaaatt
      361 ccggccgcgc aaaaggtggt gatcccggcc gccggccacg ccgtcaacat cgaccagcca
      421 caggcgttca tcgatgccgt gttgccgttc ctcgatggcc tcgacgccag gacagctcag
      481 aaagccgcat catgaagaaa ctgctcgcgg cggcattatc gatggttgcc gtatccggcg
      541 gcgcaattgc tccggtaaat gcggaagccc tgccgccgat cggtggcccc taccctccgc
      601 ccttcaccgc gactttgtcg aacaacatcc cgctggtatt cggcatggac gcggaagatg
      661 ccgcacgcgc gcttggcacg cccctcaact atgtcagggg ccgcccgggc gacgaaatct
      721 atctggcatt tcgcaatctc ggcggcagcg gtttgtttcc tcaccggcac cggctctatc
      781 tgcaattccg ccacggccga ttggccggct ggaagggcga ttggggccat aactggatgt
      841 ggcaatagaa ggcggctgcc tccttccaat cctcgcaaac gtctctccag caagatcaac
      901 ccctcaagca aatggatgac ccgtgggaaa agacatcaaa ctgacggctt cggacaattt
      961 tcaattgggc ggctatcgtg ccgaccccgc cacgccgccc aaggcggcaa tcgtggtgat
     1021 ccaggaaatc ttcggcgtca atcaccacat ccgcgcggtc tgcgaccgcc tcgcggatga
     1081 aggctatgtc gccatcgcgc cttcgatctt cgaccgcgtc gagcgcaatt tccagtgcgg
     1141 ctattcgccg gaggaagtcg cggtggcgcg caaattcgta gccaatcccg actgggcggc
     1201 gatcctgcgc gacacccagg ccgccatcga tgccgtcaaa gatgtcggtc cggtcggcat
     1261 catcggtttc tgcctcggcg gcagcgtcgc ttatgccgcc gcgacaaaac tgtcaggcct
     1321 gtcggcggcg atcggctatt acggcggcgc catcgttcgc ttcgccgacg acaagcccaa
     1381 ggtgccgacc cagcttcatt tcggggaaaa agacgcaggc atcccgctca gcgacgtcga
     1441 gactatcagg gccaagcggc ccgaggtcga gatccatgtc tatcccggcg cgcagcacgg
     1501 ctttcactgc gatgaacggg cgagttatga caagaccagc gccgatatcg cctggccccg
     1561 cagcctcgcg ttctttgcca ggcatttgaa gaagtaagcg ctggcaaaca ttccaccgac
     1621 gtcattccag ggcgcgcacc agcgcgaacc tcagatgtgc aattgcacat cggggaatct
     1681 cgagattccg ggttcgatgc tggagcctgt catcgggccg cgccttgcgc ggacccgttg
     1741 gcatcgcccc ggaatgacga agcgcgcatc tccgatgcga tccgagctct ccccgcaatc
     1801 ggaggaggtg aagagaagcg atcaggcgct gacgcccgcg ccgatcggac acgacacgcc
     1861 ggtgccgcca agtccgcaat agccggccgg attcttggcg agatattgct gatgataatc
     1921 ctcggcgaaa tagaattcgc cggcatcgag gatctcggtg gtgatcgcac catggcgatc
     1981 cgccgccagc gctttgccgt acatggcttt cgattcctcg gccgccttgc gctgcgccgg
     2041 cgtggtcaca tagatgcccg aacgatattg cgtgccgacg tcgttgccct ggcgcatgcc
     2101 ctgggtcggg tcatggccct cccagaag
//
LOCUS       DATNOI010000295        31362 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2564703,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000295 DATNOI010000000
VERSION     DATNOI010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 31362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31362
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2564703"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VN968_11495"
     CDS             <1..250
                     /locus_tag="VN968_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HXL29897.1"
                     /translation="AVKEAKMRSFVSPGELLMVEASLVHEGSGYAITAAKVRVGGELR
                     CNATITFGIVPFPNPELRVHMDAMAKRIGFPQQAITHD"
     gene            243..1451
                     /locus_tag="VN968_11500"
     CDS             243..1451
                     /locus_tag="VN968_11500"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HXL29898.1"
                     /translation="MTETKSSSPGSAEVWITGIGLATSLGEGLDANWDALNARRINVD
                     EKGFAPYIVHPFAPVNLDAQIPKKGDQRQMEAWQRIGTYAAGLALASAGVKGNKEILG
                     RMDMIVAAGGGERDLAVDCNILNMEAKGNCAPGFLNERLMNDLRPTLFLAQLSNLLAG
                     NIAIVHGVTGSSRTFMGEEAAGVDAARIALARIAAGQSDIALIGGSQNGERKDLLVLY
                     EFDDFNLRDKFAPVWARQKNAGFALGSAGAFLVIESKAHAQARGAKPYARLTNVVADL
                     AHRKHPGEVTATLEKLWSKLGKLDDKGAIITGATGAERATSEERAFLGKHPDFAVRAS
                     GTMFGHTMETQFPLGLGLAALSISRGALFPPNDPTGLEVEMSKPPTQIVVIGAGHWRG
                     EGMALVEAIS"
     gene            1479..2753
                     /locus_tag="VN968_11505"
     CDS             1479..2753
                     /locus_tag="VN968_11505"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HXL29899.1"
                     /translation="MTATRDKFGRPIVVVTGMGVVTSLGAGKTDNWKKLTAGESGIRT
                     ITRFPTDGLKTTMAGTVDFVPVEPFSSTDLSERLADIATEEAIAQSAIGSKGDFPGPL
                     FLAVAPVEVEWPQRRELGRAIGDTVDYDGMLRVSGGGRFTQYHRRFLFGSVADHLAET
                     FGTKGSPISLSTACASGASAIQLGVEAIRRGEADAALCVATDGSVNPEALVRFSLLSA
                     LSTHNDPPQAAVRPFSKNRDGFVMAEGAGALVLESYEAATARGAKILGIIAGCGELAD
                     SFHRTRSSPDGKPIIGCVRNALSDAGIVADQIDYINAHGTGTPENDKMEYLGISTVFG
                     ERTSQVPVSSNKSMVGHTLSAAGAVEAVFSLLTLEQQRIPPTINYDIPDPAIPFDVVP
                     NTARDARVTAVMSNSFGFGGQNAVLILTREPV"
     gene            2853..3875
                     /locus_tag="VN968_11510"
     CDS             2853..3875
                     /locus_tag="VN968_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXL29900.1"
                     /translation="MRALTLVADRQLVIAQVPAPPPPAADEVQIRVKAVALNHIDVWG
                     YRGMAFAQRKLPLVVGAEASGEIAAVGAGVTRFKPGRKVVMYGALTCGGCRACKEGRD
                     NLCENVAGIMGFHIDGFARELLNLGERLVLPVPDGVSERDAACAPIAFSTVQHMLFDN
                     AKLQPGETVLVHAGGSGIGTVAIMMAKAIGCTVITTVGDDSKIDGVKALGADHVINYR
                     KDRFEHETRKITKKKGVDVVFEHVGADTFNGSLLSLKRGGRLVTCGSTSGPTTTLNLM
                     QLFQQQYRIFGSFGASMRNISESLDKMAAGMHPVIDTEVPIEDVAAALKRMESRQVFG
                     KIVVGF"
     gene            3875..4807
                     /locus_tag="VN968_11515"
     CDS             3875..4807
                     /locus_tag="VN968_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HXL29901.1"
                     /translation="MHPLLLRTKARVRDATKPLGEAAVGALTIGMLRATRYFDPIKTA
                     NLFGRILRLVGPMTREQKIGRANLTAAFPEKSPAEIETILAGVWDNLGRLGAEFAHLG
                     HIWEHDPARPQDSRIEIPTRTHELFAQLRLDGKPALIFTGHLGNWELPALAAVAHGLD
                     AAILYRRPNIASADRIIEELRSVKMGTLIPAGRDAPLKLAQALQNGQHVAMLVDQYFT
                     NGVDVTFFGRKTKANPTLARLLRQIECPIHGVRIIRLPGHRFRAEISEEVQPVRDAAG
                     KIDIQGTMQAVTSVIEGWVREYPDQWLWLHRRWR"
     gene            complement(4901..6019)
                     /locus_tag="VN968_11520"
     CDS             complement(4901..6019)
                     /locus_tag="VN968_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL29902.1"
                     /translation="MICRRFRPQIPFVVAVAAVVLALVSPSARAEERTVNFYNWSNYM
                     APGVLEDFTRETGIKVVYDTFDANETLETRLLAGKSGYDVVVPTAYFLQRQITANVFQ
                     KLDKSKLPNLANAWGVVTKRLATYDPGNLYAANYMWGTTGIGYSVKAVQKILGPDARI
                     DSWDIVFKPENLAKFKECGIHMLDSADDIFPAALGYLGLDPNSTKQGDLEKAADLVIK
                     IRPYVRKFHSSEYLSALATGEICFVVGWSGDIMQARSRAAEAKSDIEIGYAIPREGAQ
                     MFFDNLAIPADAKNVAEAYELINYLYRPEVAARNSDFLSYANGNLASQKLVDPKILND
                     KNIYPDEATLKKLFVITARDPATQRVINRLWTRVKTGR"
     gene            complement(6030..7193)
                     /locus_tag="VN968_11525"
     CDS             complement(6030..7193)
                     /locus_tag="VN968_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HXL29903.1"
                     /translation="MTSMNKVFADLPVTIFEAMSQLARDNNAINLGQGFPDDPGPEDI
                     RRAAADAVMDGYNQYPSMMGIPELRQAIAAHYGRWHGLTLDPMTEVMVTSGGTEALTS
                     AILAVVEPGDEVVVFQPVYDSYLPIIRQAGGIPRLVRLEPPHWRLTEEALRSVFNHNT
                     KAVLFNNPLNPAAVVYPREDLELLARFCQEFDTVAICDEVWEHVIFDGREHIPLITIP
                     GMRDRTIKVGSAGKIFSLTGWKIGFVCAAPPLLRVAAKVHQFLTFTTAPNLQVAVAYG
                     LGKPADYFYRMRKELARSRDRLTAGLERIGFPVLRSQGTYFLTVDLSPLGLNETDEAF
                     CKRIVTDYKVAAIPVSAFYEKDAVTSVVRFCFAKQDKTLDTALERLSDAVHRR"
     gene            complement(7202..7480)
                     /locus_tag="VN968_11530"
     CDS             complement(7202..7480)
                     /locus_tag="VN968_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXL29904.1"
                     /translation="MAEGRYVLVDVREPNEVAAEAYPGAVVVPLSSFDPKDIPDPQGK
                     QVVFACRSGKRSVTASLAAQAGGLAYDKHLAGGMLAWKAAGLPTKTGG"
     gene            complement(7762..9684)
                     /locus_tag="VN968_11535"
     CDS             complement(7762..9684)
                     /locus_tag="VN968_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HXL29905.1"
                     /translation="MTSDVVIPAVETAAANGHGDAHSTAGFKALMLGSIGVVYGDIGT
                     SPLYAFREAVVAASGHSGVTPQAVLGVLSLILWALIIVVTLKYVLILLRADNHGEGGT
                     LALMALAQRAIGSAAGKIVLLGIIGGALFYGDAVITPAVSVLSAIEGMTIVTAAFEPY
                     VVALTVVILLALFAVQSRGTARVAAFFGPIMCAWFAVIAVAALPQIARHPEVLLAFNP
                     GYAVSFMIHHGIIGFITLGAVFLAVTGAEALYADLGHFGKRPIQMAWLFIVLPSLALN
                     YLGQGALALENPKTLENPFFLMFPDWALFPMVVLAIVAAVIASQAVITGAYSLTRQAI
                     QLGLMPRFEIRHTSESHSGQIYIPRVNMLLSIGVVLLIVLFRSSSALASAYGIAVTGT
                     MVVTAMMGFVVIWRVWKWSPFAAAALIAPFLFLDLTFLAANLLKVLEGGWVPLALGAV
                     VMLLMYTWRRGSRLLFEKSRKLEFPLADLVSMLEKRPPQRVSGTAVFLTSDPVSAPTA
                     LMHSLKHYKVLHEKNVILTIETAPTPRIDPAERVKLEQISATFSKVTLRFGFMESPNV
                     PKALAIARKLGWHFDIMSTSFFLSRRALKPAAHSGMPRWQDHLFISLSRTANDATDYF
                     QIPSGRVVEVGTQVTV"
     gene            complement(9832..11730)
                     /locus_tag="VN968_11540"
     CDS             complement(9832..11730)
                     /locus_tag="VN968_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HXL29906.1"
                     /translation="MAVSVTSTETQDGPVTSGFWALTLGSIGVVFGDIGTSPLYAFRE
                     AVAGASQGQPVTRVIVLGVLSLILWALFIVVTAKYVLLLLRADNNGEGGTLSLMALGQ
                     QALGRRSWPLLALGVIGASMFIGDSMITPAISVLSAVEGLKLAAPALEHYVVPLTVFI
                     LVVLFSVQRGGTARVASAFGPVMIVWFATLAVMGLVHISDDPSVLAAINPWYGIHFLL
                     SHGTIGMVTLGAVFLAVTGGEALYADLGHFGRKPIQAGWLYFVLPSLLINYFGQGALV
                     LSNPAAIENSFYRMVPEILLLPLVVLATAATVIASQAVITGAYSLTRQAVQLGLLPRF
                     EVRYTSESHAGQIYLPRVNRLLLIGVLLLVLLFRTSSGLASAYGIAVSTTMVADGIMG
                     FVVIWKLWNWRAATAAALIVPFVVVDMTFFSANLLKLLEGAWVPLLFGFAMAIMIWTW
                     RRGAAILIAKTRRTEVPLKDLIKSLEKRPPHIVKGTAVFLTSDPNFVPTALLHNLKHN
                     KVLHEHNVILTIETAQTPRVDPGERVRMEKVSDKFSTVRLRFGFMESPNVPKALVIAR
                     KLGWQFDIMATSFFVSRRSLKPSAQSGMPQWQDHLFIALSRSANDATDYFQIPTGRVV
                     EVGTQVTI"
     gene            complement(11893..12723)
                     /locus_tag="VN968_11545"
     CDS             complement(11893..12723)
                     /locus_tag="VN968_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HXL29907.1"
                     /translation="MAKKKRGAGHAGGHGWYVSFADLMGLMMSFFVMLVAFSTMDNNK
                     LKIVAGSMRDAFGVQTEARYSGIIESDGLPTRPKLKNTAHIPPEEASNTPTPDEQERN
                     KTAGARLKIDREFALASASLRQALQDLPELTEISKHVMFEETNQGLNLEIVDQDGRSM
                     FADGSKVPNDRTRRLLQKLAAPLKATPLRVSIVGHTASGFVPMQSDYGAFDLSADRAN
                     AVRQILEREGLPPAHIFAVSGRADSQPLFPDDPSLAANRRVTITLMREDPALPPDLTP
                     "
     gene            complement(12754..13527)
                     /locus_tag="VN968_11550"
     CDS             complement(12754..13527)
                     /locus_tag="VN968_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXL29908.1"
                     /translation="MDIMTSVGLVSGIIVVSVIMLMGGDIHMFISEHAVIIVVGGSIA
                     ATMIRFPLSAILHGLPLGAKFAFTMSRLSARDLVDELARIAEIARKQGPVGLEKVETD
                     EPFLAKGIRYVADGYDLEFIRDNLERDRDNFLMHLDEGGKIYRAIGDCTPAFGMVGTL
                     IGMVQMFANMTDPSKLGPFMATALLATLYGAVIANLFCLPIADKLHGKLLDEETNRTL
                     IIDGILMIRDSKSPTLVREMLLAYLPEKHRQEEGEPVPA"
     gene            complement(13785..14612)
                     /locus_tag="VN968_11555"
     CDS             complement(13785..14612)
                     /locus_tag="VN968_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL29909.1"
                     /translation="MKMAGKTVLITGSTDGVGRYVAARLAAAGANVLIHGRDQARAKT
                     LADEIRGQGHIEPVFYQADLSSIAGARQLAAAVLAGHQRLDVFISNAGIGSRSLGSGR
                     QTSADGHELRFAVNYLSGFLLAHLLLPLLKASAPSRIVNVASLGQHPLDFDDVMLTKG
                     YNGNRAYAQSKLAQIMFTIDLAQELEGSGVSVNALHPATYMNTTMVREGGITPISTVE
                     QGGEAILHLAAGDDVAGKSGLFFNGMQQAQANPQAYDAGARQRLRARSLELTGLSAG"
     gene            complement(14688..15407)
                     /locus_tag="VN968_11560"
     CDS             complement(14688..15407)
                     /locus_tag="VN968_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HXL29910.1"
                     /translation="MTNSATAPLPAALLEALARHDTPTICNALEIVAPGRRLIGYTTR
                     PLVCPFPDLPPIVGYARTVTIRSVVASGLAAAEQQARRLAYYEYVGTGQGPRITVIQD
                     IDGADVGYGAFWGEVQSAVHKALGCVGVITDGSIRDIPQWAPGFQALAGSIGPSHAYV
                     HAESFGGEVRVAGMTVRSGDLIHADRHGAVVIPFDVAAKLPDAAELCGRRETPILEIA
                     RSPSFTLEKLKEALARSAEIH"
     gene            complement(15504..16379)
                     /gene="pssA"
                     /locus_tag="VN968_11565"
     CDS             complement(15504..16379)
                     /gene="pssA"
                     /locus_tag="VN968_11565"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HXL29911.1"
                     /translation="MPVDPKYPEMRRRRFRPIPVRMLVPNVITLLAICAGLTAIRLST
                     EGRMELAVAAIVFAAVLDGLDGRVARMIKGQSKFGAELDSLADFVNFGVAPGLILYFW
                     QLHDLSNGGWIAAMVFAISGGLRLARFNATMDDPNKPAFAANYFTGVPAPAGAITVLL
                     PIYLAFLGLPMPPAMLTALYTLLIAFLMVSRLPVFSGKTVRMRVPPEMVLPVFVSVIF
                     FIALLVSYPWHIISAGSVLYLLSLPAGWKSYRDHARNAAAPATPPANVAPSSPAPPLS
                     VVTEPEQDNRPARLN"
     gene            complement(16463..17161)
                     /locus_tag="VN968_11570"
     CDS             complement(16463..17161)
                     /locus_tag="VN968_11570"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HXL29912.1"
                     /translation="MSIANSIRAQIPPVHPEGYPFIGGFALASLILFWIWTPLGWIGT
                     LLTVWCALFFRDPVRVTPIREGIVVAPADGRISMVTQVLPPAELGLGDRPLPRVSIFM
                     SVFNCHVNRSPVAGRIDRIAYRPGTFINAELDKASEDNERNSLVISTPNGRIGVIQIA
                     GLVARRIVSFVREGQTIGAGERFGLIRFGSRLDVYLPEGTTALVSEGQTAVAGETILA
                     DFKLGDPGRTYRAD"
     gene            complement(17219..19177)
                     /locus_tag="VN968_11575"
     CDS             complement(17219..19177)
                     /locus_tag="VN968_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HXL29913.1"
                     /translation="MTYTDSSPGAGQPQPSSGRSVEQATLIGTLAHLWPYIWPGDRVD
                     LKMRVVWSVVLLLVAKFATLSVPFTFKWAIDALTGKDTAPVQASNWPLWLIASPLIMT
                     LSYGAIRVLMAVLTQWRDGIFARVAMHAVRKLAYLTFVHMHELSLRFHLERKTGGLTR
                     VLERGRLGIEVIVRMVILQLVPTVVEVTLLMGVLLWQFDWRYVFATMITVVVFMYYTY
                     IATEWRIEIRRKMNESDTDANTKAIDSLLNYETVKYFSAEQREAARYDRSMEGYERAS
                     VKTYTSLAVLNTGQAVIFTAGLTATMLMCAIGVRRGTNTVGDFVMVNAMMIQLYQPLN
                     FMGMVYREIKQAVIDIEKMLGVLSRNPEVKDVPGAKPLIVTSGSVRFDDVRFSYDSER
                     PILKGLSFEVPAGKTVAIVGPSGAGKSTISRLLFRLYDISGGKILVDGQDIRNVTQSS
                     LRASIGMVPQDTVLFNDTIRYNIRYGRWDASDAEVEQAAQLAQIDDFIRMSPKGYETQ
                     VGERGLKLSGGEKQRVAIARTILKGPPILVLDEATSALDSHTEQEIQDALERVSRNRT
                     SLVIAHRLSTIVGADEIIVLDQGRIAERGTHSQLLATGGLYASMWNRQREAQEARERL
                     ARIADENEAPNRAPPLVDDALATPAAAE"
     gene            19400..20005
                     /locus_tag="VN968_11580"
     CDS             19400..20005
                     /locus_tag="VN968_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HXL29914.1"
                     /translation="MNKIKTVCVYCGSGPGTNPRFVEAAIALGKILADNGVSLVYGGG
                     SNGLMGAIAKSTLDHGGNVIGIIPEFLISRENAMARVQELIVTPDMHERKRTMFERSD
                     AFVALPGGIGTLEELVEQLTWQQLGRHAKPVLLANIDSFWEPLLALLTHMRATEFIRP
                     TLAVEILKAEHVEDILPRLLAAAALAPEGTDEMAAEVIRQL"
     gene            complement(20017..20415)
                     /locus_tag="VN968_11585"
     CDS             complement(20017..20415)
                     /locus_tag="VN968_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL29915.1"
                     /translation="MIDHVSVGVSDLERAARFYELALAPLGLSRLVTRPATVGFGKNY
                     PEFWINFRAGMARVQPESGTHICLRAKTADDVDAFHAAALHAGGRSDGAPGLRPHDRV
                     RYYAAFVIDPDGNRIEAVTFPKDDAEVSPR"
     gene            complement(20531..22129)
                     /gene="cimA"
                     /locus_tag="VN968_11590"
     CDS             complement(20531..22129)
                     /gene="cimA"
                     /locus_tag="VN968_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HXL29916.1"
                     /translation="MTRERLYLFDTTLRDGAQTNGVDFTLHDKQLIAKMLDELGIDYV
                     EGGYPGANPTDTEFFAEKPDLAHARFTAFGMTRRPGRSVSNDPGLAALLEARADAICF
                     VAKSSAYQVRVALETTNDENLASIRDSVKAAKSAGREVMLDCEHFFDGFKENAAFALS
                     CAKAAYDSGARWVVLCDTNGGTMPHEVEAIVTEVLKHIPGDHVGIHAHNDTEQAVANS
                     LAAVRAGVRQIQGTLNGLGERCGNANLCSLIPTLRLKREFSDCFDIGVSPDKMATLMK
                     VSRTLDDMLNRAPNRHAPYVGESAFVTKTGIHASAVLKDPQTYEHVAPEAVGNRRKVL
                     VSDQAGRSNVIAELERAGIAYEKNDSKLVRLVEELKEREAAGYAYESANASFDLLARR
                     TLGRVPEYFRVEQFDVNTEQRYNANGQRVTVAMAVVKVDVAGEHLISAAEGNGPVNAL
                     DVALRKDLGKYQKYIEGLRLIDYRVRILNGGTEAVTRVLIESEDEEGKRWTTIGVSPN
                     IIDASFQALMDSVIYKLVKSGAPA"
     gene            complement(22126..22638)
                     /locus_tag="VN968_11595"
     CDS             complement(22126..22638)
                     /locus_tag="VN968_11595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL29917.1"
                     /translation="MGQALPKPALRPFLAADTPMLAAIFVASVEELTGDDYSEAQQQA
                     WASAADDEEQFGKRLASELTLIATLQNSPVGFASLKGADHIDMLYVHPGAVGQGVASM
                     LCDALEKLAGSRGARSLSVDASDNAQEFFLKRGYVGKQRNTVTVNGEWLANTTMQKTL
                     VQGPSSGVPT"
     gene            complement(22638..22802)
                     /locus_tag="VN968_11600"
     CDS             complement(22638..22802)
                     /locus_tag="VN968_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29918.1"
                     /translation="MNPSEIGVIAGLDPAIHQLKNSAAVVWTDARVEPAHDGRGCWQP
                     MRNLFLDEIV"
     gene            complement(22799..24193)
                     /gene="cysS"
                     /locus_tag="VN968_11605"
     CDS             complement(22799..24193)
                     /gene="cysS"
                     /locus_tag="VN968_11605"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HXL29919.1"
                     /translation="MDLRLYDTLTREKRIFVPLDPANVRMYVCGPTVYDFAHIGNARP
                     VIVFDVLFRLLRHIYGENHVTYVRNITDVDDKINDRAVRDFPGLPLNEAIRKVTEKTA
                     AQFASDVAALGSLPPTFEPRATDFVLPRADGKADMVTLIKQLIARGHAYEAGGEVLFD
                     VQSMPDYGALSGRKLDEQRPGARVAVDAHKKSPLDFVLWKQSSGDEPGWESPWGRGRP
                     GWHIECSAMSAAYLGEVFDIHGGGIDLIFPHHENEIAQSRCAHGTPAMANYWMHNEFV
                     QVEDAKMSKSLGNFVTIRELLADWPGEVLRLNMLKAHYRSPMDWTSKGLEESAKTLDD
                     WYWVAADNKGERPSEAVIEALSDDLNTPQMIASLHGLRNSAASGSERDRNAFAASLRL
                     LGFLSESAAEWKGRKQQASGVDVKQIDGLISDRAAARARKDFQESDRIRDELAAMGVV
                     IKDSKEGTTWEIAR"
     gene            24470..24778
                     /locus_tag="VN968_11610"
     CDS             24470..24778
                     /locus_tag="VN968_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29920.1"
                     /translation="MRSLPALLACTFLLGASAASADSRVFIIANQADGYGVDQCLARG
                     DQCGAYAARSYCQSRDFTQASAYRRVDPDEVTGSVPKPAGGSCNHAGCDEYVAITCQR
                     "
     gene            24951..26090
                     /locus_tag="VN968_11615"
     CDS             24951..26090
                     /locus_tag="VN968_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HXL29921.1"
                     /translation="MPETLITPFARWIFACAALFGAAVLSVDASAQMSQAPPPPQQQG
                     APVNPMCPRLEAQLASIDRGGGDPAKDEQIRRYQDAATKQQGELDRVTSQAKRMGCDS
                     SGFFSLFSGQSAQCGPVNTQIQQMRANLEQITTSLERLRTGGVGGADRDNQRRSVLTA
                     LAQNNCGPQYANAARGSGNFLENLFGNNNNNPVAPPGADLGAQSGTYRTVCVRSCDGA
                     YFPISFATVPGRFPDDERTCKALCPAAEATLYSYRNPGEDMNQAMSINGQPYSSSPNA
                     FRYRQEFNPSCACKAAGQTWSDALKSIDDKAEAEQQGDIIVTEESAKKMARPLTKGAA
                     TKKGAAATTATAPAPEASPPAADSTGAAPDNKPIRSVGPTFIPAR"
     gene            complement(26101..27858)
                     /locus_tag="VN968_11620"
     CDS             complement(26101..27858)
                     /locus_tag="VN968_11620"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HXL29922.1"
                     /translation="MTERAAQFAITLGQLNPTVGDVAGNAAKARAARAQARLDGADLL
                     VLPELFITGYPPEDLVLKPAFQAACRAAVEELARDTADGGPAVLIGSPWVEDGKLYNA
                     CALLDEGRIAAIRFKANLPNYGVFDEKRLFARGPAAGPVTIRGVRVGVPICEDIWLEE
                     SEDYENVVECLAETGAEILVVPNGSPYARDKNDLRLSIAVARVTESGLPLIYLNQIGG
                     QDELVFDGASFALNADLSVAAQLPAFEENITTLRWSKGEAGWRCTGPMAPLVEGDKGD
                     YAACVLGLRDYVRKNGFPGVLLGVSGGIDSALCAAIAVDALGAAQVRGVMLPFRFTAQ
                     VSLDDAAKLALALGIRYEVLPIAQAVNGFEEILSKTFAGLPRDITEENLQARTRGTFL
                     MAISNKTGAMVVTTGNKSEMSVGYATLYGDMNGGYNPIKDIYKTEVFRLSSLRNAWKP
                     DGALGPSGEVIPVNIITRPPTAELRENQTDQDSLPPYDMLDAILERLVEREEPLASIV
                     AAGFDREVVARIDRLLNIAEYKRRQAAPGVKVTQKNFGRDRRYPITNKFRDLANALPE
                     PDDTLVSRASRGSAEAFEG"
     gene            complement(27941..28294)
                     /locus_tag="VN968_11625"
     CDS             complement(27941..28294)
                     /locus_tag="VN968_11625"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HXL29923.1"
                     /translation="MLRFWRATINTRNGLVFAIRSEQAVREELTALALALPLAWLIGA
                     TTMRRVELIAVVVLVLVVELLNTAIEKLADRLTTEHDPQIGRVKDMGSAAVGVALVMA
                     GLFWLFAIAERMGAV"
     gene            complement(28296..29681)
                     /locus_tag="VN968_11630"
     CDS             complement(28296..29681)
                     /locus_tag="VN968_11630"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HXL29924.1"
                     /translation="MSERWTPDSWRARPVKQVPDYPDAKALADVEAQLATFPPLVFAG
                     EARNLKRALARVAAGDAFLLQGGDCAESFAEHGANNIRDYFRVFLQMAIVLTYAGALP
                     VVKIGRVAGQFAKPRSSPIETIDGVELPSYRGDIVNDIAFTAQARTPDPRRQIEAYRQ
                     SAATLNLLRAFAKGGYASVDNVHQWMLESVKDSPQSKRYMDLADRISEALDFMRACGL
                     NFAVDSSLGTTDFYTSHEALLLGYEQAFTRVDSTSGGYYATSGHMIWIGDRTRQLDHG
                     HVEYFRGIKNPIGLKCGPSLKPDELLRLIDVLNPDNDPGRLTLINRFGSDKIGDHLPQ
                     LIRAVQREGRVVVWSCDPMHGNTISSNSGYKTRPFDRILSEVKSFFSIHAAEGSYAGG
                     VHLEMTGQDVTECIGGARAITHEDLSDRYHTACDPRLNAEQSIDMAFLIAELLKGRGG
                     RAKPMPVAAGL"
     gene            29908..30759
                     /locus_tag="VN968_11635"
     CDS             29908..30759
                     /locus_tag="VN968_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL29925.1"
                     /translation="MDGYLLGATLFLPLGAKRHAVLINSATAVPRRIYRGFAGYLARR
                     GCAVLTYDYRGTGDSRKKSPAGSRRPRSLAGFKAGMSDWAALDVTAAVAWMRERYHHL
                     PLNYVGHSFGGQALGLLPNNSEVSRALLIAAQAGYWKLMAAPERYRVYVMLNYVGTPL
                     TRVLGYAPGWSGLGEDLPRGVFQQWVGWVMSRRYLFDDTNLKALQNFPQFRGAMRALC
                     FSDDPWATPPAVELLCSGFIAIDPEILTIAPADVGAKRIGHFGFFRPEHRDTLWRGAA
                     EWIQAAE"
BASE COUNT         6152 a         9888 c         9564 g         5758 t
ORIGIN      
        1 cgcggtcaag gaagccaaga tgcgaagttt cgtcagcccc ggcgaactgc tcatggtcga
       61 ggcgagcctc gttcacgaag gttcgggtta tgcgattacg gccgccaagg tccgcgttgg
      121 cggcgaattg agatgcaacg ccacgatcac gttcggcata gtccccttcc cgaatccgga
      181 actgcgcgtg cacatggacg cgatggccaa gcgtatcggt tttccgcagc aggccatcac
      241 ccatgactga gacgaaatcc tcgagcccgg gatcggcgga agtctggatc accggtatcg
      301 gtctggcgac ctcgcttggc gaagggctgg acgccaattg ggacgcgctc aatgcgcggc
      361 gcatcaacgt cgacgagaag ggtttcgcgc cctacatcgt tcatcccttt gccccggtca
      421 acctggacgc gcagatcccc aagaagggcg accagcgcca gatggaagcc tggcagcgga
      481 tcggaaccta tgccgccggg ctggcgctgg cttcggccgg cgtgaagggc aacaaggaaa
      541 tcctggggcg gatggacatg attgtcgccg ccggcggcgg cgagcgcgat ctcgcggtgg
      601 actgcaacat tctgaatatg gaagccaaag gcaactgcgc tccgggattt ctcaatgagc
      661 ggctgatgaa cgatctgcgc ccgacgcttt tcttggcgca gctttcgaac ctgctggccg
      721 ggaacattgc catcgtacac ggcgtaaccg gatcgtcgcg taccttcatg ggcgaggaag
      781 ccgcgggcgt tgacgcagca aggatcgcgc tcgcacggat cgccgccggt cagagcgaca
      841 tcgcgctgat cggcggctcg caaaacggcg agcgcaagga tttgctggtt ctctatgaat
      901 tcgacgattt caatctccgg gacaaatttg ctccggtttg ggcgcgccag aaaaacgccg
      961 gtttcgcact tggctccgcc ggcgcgtttc ttgtcatcga gtccaaagcc catgcacagg
     1021 cgcgcggcgc gaaaccttat gcgcgcctga ccaacgtcgt cgccgatctg gcgcaccgca
     1081 agcaccctgg cgaagtcacg gcaacgctgg aaaaactgtg gtcgaagctc ggcaagcttg
     1141 acgataaggg cgccatcatc accggcgcga ccggcgccga acgggccact tccgaggagc
     1201 gggcgttcct gggcaagcat ccggattttg cagtgcgggc cagcggcacc atgttcgggc
     1261 acacgatgga aacccagttt ccgctcggac tgggcctcgc cgcactctcg atatcacgcg
     1321 gggctttgtt tccgcccaac gatccgacag ggctggaggt tgaaatgtcc aagccgccga
     1381 cccagattgt cgtcatcggg gccggtcatt ggcggggcga aggcatggcc ctggtcgagg
     1441 cgatcagttg agcacggcgc tggacgggcg gggaaaacat gacagctacg cgcgataaat
     1501 tcggcaggcc gatcgtcgtc gtcaccggca tgggtgtcgt gacatcgctc ggcgccggca
     1561 agaccgacaa ctggaaaaaa ctcaccgccg gcgaatccgg cattcgaacc attacgcgtt
     1621 ttcccaccga cggcctgaaa acgaccatgg ccggaacggt cgatttcgtg ccggtcgagc
     1681 ctttttcctc caccgatctt tcggagcggc ttgccgacat cgccacggaa gaagccatcg
     1741 cgcaatccgc gatcggcagc aaaggtgatt ttcccgggcc gctgtttctt gctgtcgccc
     1801 ctgtcgaggt cgagtggccg cagcggcggg agctgggacg cgcgatcgga gacacggtcg
     1861 attatgatgg catgctccgt gtcagcggcg gcggaaggtt cacgcaatat caccggcggt
     1921 ttctgttcgg ttcggtggca gatcacctcg ccgagacatt cggcaccaag ggttcgccga
     1981 tttcactttc gacggcatgc gcgtccggcg ccagtgccat ccagctcggc gttgaagcca
     2041 ttcgccgcgg cgaagccgac gccgcgcttt gcgtcgcaac cgatgggtcg gtcaatccgg
     2101 aagccctggt ccggttctcc ttgctgtcgg cgctatcgac ccacaacgat ccgccgcagg
     2161 cggcggtccg gccgttctcg aagaatcgcg atggcttcgt gatggcggag ggcgccggcg
     2221 cgctggtgct ggaaagctac gaggccgcca cggcgcgcgg cgcaaagatc cttggaatca
     2281 tcgccggctg cggagaactg gcggattcat tccaccgcac ccgatcgagt cccgacggca
     2341 agccgatcat cggatgcgtg cgcaatgcct tgtcggatgc cggcattgtc gccgaccaga
     2401 tcgactacat caacgcccac ggcaccggaa cgccggagaa cgacaaaatg gaatatctgg
     2461 gcatctcgac ggtgttcggc gagcgcacca gccaggttcc ggtttcctcc aacaagtcga
     2521 tggttggcca taccctctcc gcggcgggcg cggtcgaggc ggtgttctcg ctgctgacgc
     2581 ttgagcagca gcggattccg ccgaccatca attacgatat tccggacccg gcgataccct
     2641 tcgacgtggt tcccaacacc gcgcgcgatg cccgcgtcac cgcggtgatg tcgaattcgt
     2701 tcggcttcgg cggccagaat gctgtgctaa ttctgacgcg cgaaccggtt taagcggctt
     2761 gatcccttgc aatgccggcg gttgacgtca tcgtaccaac cggcgatacg cccccgatca
     2821 tccatccttt gtcctgccgg agacacggtc tcatgcgtgc gctcactctc gttgccgatc
     2881 gtcagctggt gattgcgcaa gtgccggcgc cgccgccgcc ggcggcggac gaggtgcaga
     2941 tccgcgtcaa ggcagtggcg ctcaatcaca tcgatgtctg gggctatcgc ggcatggcgt
     3001 ttgcccagcg caagctgccg ctggtggtcg gcgcagaagc ctccggcgag attgccgccg
     3061 tcggcgcggg cgtcacgcgc ttcaagccgg gacggaaggt ggtcatgtac ggcgccctca
     3121 cctgcggggg ttgtcgtgcc tgcaaggaag gccgcgacaa tctttgcgag aacgttgcgg
     3181 gcattatggg cttccatatc gacggtttcg cgcgcgaact tttgaacctc ggcgaaagac
     3241 tggtgcttcc agtgccggat ggcgtcagcg agcgtgacgc ggcctgcgcg ccgatcgcat
     3301 tctcgaccgt gcagcacatg ctgttcgaca atgcaaaact gcaacccggc gaaaccgtgc
     3361 tggtgcacgc cgggggctcc ggcatcggga ccgtcgccat catgatggcc aaggcgatcg
     3421 gctgcaccgt catcaccacc gtcggggacg attccaagat cgacggcgtc aaagcgctcg
     3481 gcgccgatca tgtcatcaat tatcgcaagg accggttcga gcacgagacc cgcaagatca
     3541 ccaaaaagaa gggcgtcgac gtcgtgttcg agcacgtcgg cgccgacacg ttcaacggct
     3601 cgttgctttc gctgaaacgc ggtggacggc tggtgacctg cggctcgacc tcaggaccga
     3661 ccacgacgct caacctgatg caattgttcc agcaacagta tcggatcttc ggctcattcg
     3721 gcgcgtcgat gcgcaatatt tccgaaagcc tcgacaaaat ggccgccggc atgcatccgg
     3781 tgatcgacac cgaagtgccg atcgaggatg ttgccgccgc gctgaagcgc atggaaagcc
     3841 ggcaagtgtt cggcaagatc gtcgttggtt tctgatgcat cccctcctgc ttcgcaccaa
     3901 ggcccgtgtc cgcgacgcga ccaagccgct cggcgaggcc gcggtgggcg cgttgacgat
     3961 cggaatgctg cgcgccaccc gctatttcga tccgatcaag accgcgaacc tgttcggccg
     4021 gattctgcgc cttgtcggac cgatgacgcg cgagcaaaag atcggccgcg ccaatctcac
     4081 cgccgcgttc ccggaaaaat cgcctgcgga aatcgagacc attctcgccg gtgtctggga
     4141 caatctcggc cgcctcggcg ccgaattcgc gcatctcggc catatctggg agcacgaccc
     4201 cgcgcgcccc caggatagcc ggatcgagat cccgacgcgc acccacgaat tgttcgccca
     4261 actgcggctc gacggcaagc ccgcgctgat ctttaccggc cacctcggca actgggagct
     4321 gccggcgctg gctgcggtcg cgcatgggct ggatgccgca atcctgtatc gccggcccaa
     4381 catcgcttcg gctgaccgca tcatcgaaga gctgcgctcg gtgaagatgg gcacgctgat
     4441 tccggccggc cgcgacgctc cgctgaagct cgcgcaggcc ctgcaaaatg gccagcacgt
     4501 tgccatgctg gtcgaccagt actttactaa tggcgtcgac gtcacgttct tcggccgcaa
     4561 gaccaaggcc aatccgacgc tggcccggct gttacggcag atcgagtgtc cgatccatgg
     4621 cgtgcgcatc atccgcctgc ccggccatcg ttttcgcgct gaaatatccg aagaagtgca
     4681 gccggttcgc gacgccgccg ggaaaatcga tatccagggg acgatgcagg cggtgacctc
     4741 ggtgatcgag ggctgggtcc gcgaatatcc cgaccagtgg ctgtggctgc atcgccgctg
     4801 gcggtagctc tccagcttgt gcgtgcagca ttgcagcgag aaaaacttgg atggccggga
     4861 caagcccggc catgacgggg aaagttcttg gacgacggcc ttaccttccc gtcttcaccc
     4921 gcgtccacag ccggttgatg acgcgctgcg tcgccggatc gcgcgcggtg atcacaaaaa
     4981 gcttcttgag cgtcgcctcg tccggataga tgttcttgtc attgaggatt ttcgggtcga
     5041 ccagcttctg gctcgccaga ttgccgttgg cgtaagacag aaaatcggaa ttccttgccg
     5101 cgacctcggg ccggtagaga tagttgatca actcataggc ctcggccaca ttcttcgcat
     5161 cggccgggat cgcgagattg tcgaagaaca tctgcgcgcc ttctcttgga atggcgtagc
     5221 cgatctcgat gtcgcttttt gcctcggcgg cgcggcttcg cgcctgcatg atgtcgcccg
     5281 accagcccac cacgaagcaa atctcacctg tcgccaatgc gctcagatac tccgacgaat
     5341 gaaattttcg gacatagggc ctgatcttga tgacgagatc ggcggccttt tcgagatcgc
     5401 cctgtttggt cgagttcgga tcgagcccga gataaccgag cgccgcggga aaaatatcgt
     5461 cggcggaatc cagcatgtga atgccgcact ccttgaattt tgccaggttc tccggcttga
     5521 acacgatatc ccagctgtcg atcctggcgt cgggaccgag gatcttttgc accgccttga
     5581 cgctgtagcc gatcccggtg gtgccccaca tgtagttggc ggcatagagg ttgccgggat
     5641 cgtaggtggc cagccgcttc gtcacgacgc cccacgcgtt ggcgaggttc ggcagtttcg
     5701 atttatcgag cttctggaaa acattcgccg tgatttgccg ctgcaaaaaa taggcggtgg
     5761 gaacgacgac atcgtaaccg gattttcccg cgagcaaccg tgtctccagg gtctcgttgg
     5821 cgtcgaacgt gtcgtagacg accttgatgc cggtttccct ggtgaagtct tcgagcaccc
     5881 ccggcgccat gtagttcgac caattgtaga aattgacggt gcgctcttcc gccctcgcgg
     5941 acggtgaaac cagcgcaagg accaccgcgg caaccgcgac aacaaacggg atttgagggc
     6001 ggaaccgtcg gcatatcatc tgctatctct catcggcggt gcacggcatc cgacaggcgt
     6061 tccagcgcgg tgtcgagcgt cttgtcctgc ttggcgaaac agaaacgcac caccgacgtc
     6121 accgcatcct tttcatagaa ggccgacacc gggatcgccg cgaccttgta gtcggtcaca
     6181 atgcgcttgc agaaggcctc gtcggtttcg ttgagaccga gcggcgaaag atcaacggtg
     6241 agaaaatagg tgccctgcga ccgaagaacg ggaaatccga tgcgctccaa tcccgcggtg
     6301 agacgatcac ggctgcgcgc cagttccttg cgcatgcgat aaaaataatc cgccggcttg
     6361 ccgaggccat aggccaccgc gacctgaaga ttgggcgcgg tggtgaaggt gagaaactgg
     6421 tgcaccttcg cagcgacgcg caacagcggc ggtgcggcac agacgaagcc gatcttccag
     6481 cccgtcagcg aaaaaatctt gcccgcggaa cctaccttga tggtgcggtc gcgcattccc
     6541 ggaatcgtaa tcagcggaat atgttcgcgg ccgtcgaaga tcacatgttc ccagacctcg
     6601 tcgcagatcg caaccgtatc gaactcctga cagaaccgcg ccagcaattc gagatcttcg
     6661 cgcggataga ccaccgcggc tggattgagc ggattgttga agaggaccgc cttggtgttg
     6721 tggttgaaaa cgcttcgcag cgcttcttcc gtcagccgcc aatgcggcgg ttcgagccgc
     6781 accaggcgcg gtatgccccc ggcctggcga atgatcggca ggtaggaatc atacaccggc
     6841 tggaaaacca ccacctcgtc gccgggctcg accacggcga ggatcgcgct ggtcagcgct
     6901 tcggtgccgc cggacgtcac catcacctcg gtcatcgggt cgagggtgag gccatgccag
     6961 cgcccgtagt gagccgcgat cgcctggcgc agttccggaa tgcccatcat cgatgggtat
     7021 tgattgtagc cgtccatgac agcatccgcc gccgcgcggc gaatatcctc ggggccggga
     7081 tcatcgggaa aaccctggcc gagattgatg gcgttgttgt cgcgcgcgag ctgcgacatc
     7141 gcctcgaaga tggtcacggg aagatccgca aaaaccttgt tcatcgaagt catggggaag
     7201 gtcagccgcc ggtcttggtc ggcagtcccg cggccttcca ggccagcatg ccccccgcga
     7261 gatgtttgtc gtaagccaac ccgccggctt gcgcggccaa cgacgccgtt accgatcttt
     7321 tccctgaccg gcaggcaaaa accacctgct tgccctgggg atcgggaata tccttcggat
     7381 cgaaactcga gagtggaacg acgaccgcgc cgggataggc ttcggccgcg acctcgttcg
     7441 gctcgcggac gtcgacgaga acgtagcggc cctcggccat tccctttgat acctcttccg
     7501 gcgtcaaatc gtgaacctgc ccctgatccg ccactcaaat cctcccagtc tcatctcttt
     7561 cagcatgatc tcaggccaaa acgcgtcgcg tttgtccgga gggaaaaccg gttcccaccc
     7621 cccggatcaa gtcagagggc atgcttttcc ggatcatgct ccagcacgag cgaattcggc
     7681 aatgcgcggt tgctcgccct tgcgcgccaa cctcgcccct cgcgcggcga aaatcaagcg
     7741 ccggaaccgt cttcaagaac ctcaaaccgt gacttgcgtc cccacctcga ccacccggcc
     7801 gctcggaatc tgaaaataat cggtcgcgtc attggcggtg cggctgagcg atatgaaaag
     7861 gtgatcctgc cagcgcggca ttcccgaatg cgcggccggc ttcagcgcac gccgcgagag
     7921 gaagaacgac gtcgacatga tgtcgaaatg ccagcccagc tttcgcgcga tcgccagcgc
     7981 tttcggcacg ttcggcgatt ccatgaagcc aaaccgtagc gtgaccttgg agaaggtcgc
     8041 gctgatctgt tccagcttga cgcgctcggc ggggtcgatc cgtggcgtcg gtgcggtctc
     8101 gatggtgaga atgacgttct tctcatgcag cactttgtag tgtttcagac tgtgcatcag
     8161 ggcggtcggg gcgctgacgg gatcgctggt caggaatacg gcggtgccag aaacccgctg
     8221 cggcggccgc ttctccagca tcgacaccag atcggccagc ggaaactcca gcttgcgcga
     8281 cttctcgaac aatagccggc tgccgcgccg ccaggtgtac atcagcagca tcaccacggc
     8341 gcccagcgcc agcggcaccc agccgccttc gagtactttc aagaggttcg ccgcgagaaa
     8401 ggtgagatcg agaaacagaa atggcgcaat cagcgccgcg gccgcaaacg gcgaccattt
     8461 ccagactcgc cagatcacga caaaacccat catggccgtg accaccatgg ttccggtgac
     8521 cgcgatgccg taggccgacg ccagtgcact tgatgagcgg aacaggacga tgagcagaac
     8581 cacgccgatc gagagcagca tattgaccct tggaatgtag atttgcccag aatgggattc
     8641 cgaggtgtgc ctgatttcaa accgcggcat caaccccagt tgaatggcct ggcgggtcaa
     8701 cgaataggcg ccggtaatca ccgcctggct cgcgatcacg gcggcaacga ttgcaagtac
     8761 aaccatcgga aacagcgccc aatccgggaa catcaggaag aaaggatttt cgagcgtctt
     8821 cggattttcc aaagcgagcg cgccctgccc cagataattg agcgccagcg acggcagtac
     8881 gatgaaaagc catgccatct gaatcggacg tttgccgaaa tgtccgaggt cagcgtagag
     8941 cgcctccgcc ccggtcacgg caagaaagac ggcccccagg gtaataaatc cgatgatacc
     9001 gtgatggatc atgaatgaga cggcatagcc agggttaaag gccagcagca cttcgggatg
     9061 ccgtgcgatc tgcggcaagg cggccactgc gatcacggcg aaccacgcac acatgatcgg
     9121 tccgaaaaaa gccgcgactc gagccgtgcc gcgcgattgc accgcaaaca gcgcgagcaa
     9181 tatcactacc gtcagcgcca ccacatacgg ttcaaacgcg gcggtgacta ttgtcatacc
     9241 ttcgatcgcc gagagcacgg agaccgccgg cgtgatcacc gcgtcgccat aaaaaagagc
     9301 accaccaatg attccgagaa gaactatctt gccggcagcc gaacctattg cgcgttgtgc
     9361 cagcgccatc aacgccaggg ttccgccctc gccgtggtta tccgcgcgca gcaggatcag
     9421 cacatatttc agcgtcacca cgatgatcag cgcccacagg atcagggaca ggacgccgag
     9481 taccgcctgt ggagtgacgc ccgagtgccc actggccgca acaacagctt cgcggaacgc
     9541 gtaaagcgga ctggtgccga tgtcgccata gacgacgccg atgctgccaa gcatgagtgc
     9601 cttgaagccg gcggtggaat gcgcatcgcc atgcccattg gccgccgcgg tttccacggc
     9661 gggaattaca acatcgctgg tcatcgggac ggaggcctca aaaatgcttc actgcaccat
     9721 agcaaaggga ggcggcttat agccctgcgc tgagagcata gcctaccctg atttcgggcc
     9781 ctcgccatgc aaaaatgcat gaagcgttag gttgcaaacc tggattggta ctcaaattgt
     9841 gacttgggtg ccgacttcaa ccacgcgccc ggtcggaatc tggaaatagt cagtggcgtc
     9901 gttcgccgac cggctgagcg caatgaacag gtggtcctgc cactgcggca tccccgactg
     9961 cgcggatggc ttcagcgagc gccgcgagac gaaaaacgac gtcgccatga tatcgaactg
    10021 ccagccgagc ttgcgcgcga tcaccagcgc cttgggaacg tttggcgatt ccatgaaacc
    10081 gaagcgcagc cggacggtgg aaaatttgtc gctgaccttt tccatccgga cccgctcgcc
    10141 cggatcgacg cgcggggtct gcgcggtttc gatggtcagg atcacattgt gctcgtgaag
    10201 caccttgttg tgcttgagat tatgcagcag cgccgtgggc acgaaattcg gatcgctggt
    10261 gagaaaaacc gcggtgccct tgacgatgtg cggtggacgc ttttcgagac tcttgatcag
    10321 gtcctttagc ggaacttccg tgcgccgggt tttggcgatc aggatcgcgg caccccgccg
    10381 ccaggtccag atcatgatcg ccatggcaaa cccgaacagc agcggcaccc acgcgccctc
    10441 aagcagcttc aaaagattgg cgctgaagaa cgtcatgtcg accacgacga acggaacgat
    10501 cagcgccgcc gcggtggccg cgcgccagtt ccacagcttc cagatcacga cgaaacccat
    10561 gatgccatcg gccaccatgg ttgtggaaac cgcgatgcca taggcggagg ccaatccgct
    10621 tgaggtacgg aacagcagga ccagcagcaa gacaccgatc agcaacagcc ggttcacgcg
    10681 cggcaaataa atctgtccgg cgtgcgactc ggaggtgtag cggacctcga agcgcggcag
    10741 caggccaagc tgcacggcct gacgggtcag cgaatacgct ccggtgatca ccgcctggct
    10801 cgcgatcacg gtcgcggcgg ttgccagcac caccagcggc aacagcaata tctccggaac
    10861 catccgatag aaggaattct caatcgccgc gggattggac agcaccagcg cgccctgacc
    10921 gaaatagttg atcaaaagcg aaggcagcac gaaataaagc cagccggcct gaatcggctt
    10981 gcgtccgaag tgacccaaat ccgcatagag cgcctcgccg ccggtcaccg cgagaaatac
    11041 cgcccccagc gtcaccattc cgatcgtgcc gtgggagagc aggaaatgaa tgccgtacca
    11101 gggattgatc gcggccagca ccgacggatc gtcgctgata tgaacgaggc ccatcaccgc
    11161 cagcgtagcg aaccagacga tcatgaccgg cccaaaagcc gatgcgaccc gggccgtgcc
    11221 cccgcgctgg accgagaaca gcacgacgag gatgaaaacc gtcagcggca ccacataatg
    11281 ttcaagcgcc ggcgctgcga gcttgaggcc ttccaccgcg gacaggaccg aaatcgccgg
    11341 cgtgatcatg gaatcgccga tgaacatcga agcgccgatg acgccgagcg ccagcaatgg
    11401 ccagcttctc cgccccagcg cctgttgacc cagcgccatc agcgacagcg tgccgccctc
    11461 gccgttattg tcggcgcgaa gcagcagcag gacgtatttc gcggtgacga cgataaaaag
    11521 cgcccacagg atcagcgaca gaacccccag cacaatgacg cgcgtgaccg gctggccctg
    11581 cgaagcaccg gcgaccgctt cgcgaaacgc gtacaacggc gaggttccga tatcgccgaa
    11641 caccacgccg atacttccga gcgtcagcgc ccaaaaacct gacgtgaccg gaccgtcctg
    11701 ggtttcggtt gatgtgacgc tgacagccat gatgctggaa aaacgctctg tgggttgatt
    11761 tgatccgggc cggctatcga cgcttctgga aagcctgtca accgatcaac catagcatca
    11821 ccggcgatgc cgcgacggcc cacgcagcgg cttcatcaga acaatccatg gtaacatggt
    11881 agtgtgattg aattacggcg tcagatcggg cggcagcgcc ggatcctcgc gcatcagtgt
    11941 gatggtgacg cgacggttcg ccgccaatga cggatcgtcg ggaaacagcg gctggctgtc
    12001 ggcccttccc gagaccgcga aaatatgggc cggcggcagc ccttcccgct ccagaatctg
    12061 gcgcaccgcg ttggcccgat ccgccgagag atcgaacgcg ccgtagtcgc tctgcatcgg
    12121 cacaaaacct gaggcggtgt gaccgacgat ggaaacgcgg agcggcgtcg ccttgagcgg
    12181 agctgcaagt ttctgaagca gccggcgggt gcggtcattc ggcaccttgg agccgtcggc
    12241 aaacatcgac cggccatcct ggtcgacgat ttccaggttg agaccctggt tggtctcctc
    12301 aaacatcacg tgttttgaaa tttcggtcag ttccggcagg tcctgcaacg cctgccgcaa
    12361 tgatgccgaa gcgagtgcga attcgcgatc gatcttcagt cgggcgccgg cggtcttgtt
    12421 ccgctcctgt tcgtccgggg tcggcgtgtt ggatgcttcc tccgggggaa tgtgcgcggt
    12481 gtttttcagc ttcggccgtg tcggcaggcc atcggattcg atgattccgg aatagcgcgc
    12541 ctcggtctgc acgccgaaag cgtcgcgcat cgagccggcc acaattttca gcttgttgtt
    12601 gtccatggtg gagaacgcga cgagcatcac gaagaaactc atcatcagac ccatcaggtc
    12661 ggcaaaggac acgtaccagc cgtgccctcc agcatgtccg gcgccacgtt ttttcttggc
    12721 catcgttgat tttccgaatc cgaggtcggc cgttcaagcc gggaccggtt cgccctcttc
    12781 ttggcggtgt ttttcgggca gataggccag caacatttcc cgcaccagcg tcggactttt
    12841 ggaatcccgg atcatcagaa tgccgtcgat gatcagcgtg cgattggtct cctcgtcgag
    12901 caatttgcca tgcagcttgt cggcgatcgg caggcagaac aggttcgcga tcaccgcgcc
    12961 gtacagcgtc gccagcaagg cggttgccat gaacggaccg agcttcgaag gatcggtcat
    13021 gttggcaaac atctgaacca tgccgatcaa tgtgccgacc atgccgaacg ccggcgtgca
    13081 gtcgccgatg gcgcggtaga ttttcccgcc ttcgtcgaga tgcatcagaa agttgtcgcg
    13141 atcgcgctcg agattgtccc tgataaattc gagatcgtag ccgtcggcga catagcgaat
    13201 tcccttggcg agaaacggct cgtcggtctc gactttttcg aggccgaccg gcccctgctt
    13261 gcgggcgatt tcagcgatgc gggcgagttc gtcgacgaga tcgcgcgcgg agagccggct
    13321 catcgtgaag gcgaatttag cgcccagcgg cagaccgtgc aggatagcgc tgagcggaaa
    13381 cctgatcatg gtcgcggcga tcgatccgcc gacgacgatg atgacagcat gctcgctgat
    13441 gaacatgtga atatcgccgc ccatgagcat tatcactgat acaacaatga tgccggacac
    13501 gagcccaacg ctcgtcatga tatccatggg gaactccaac gcgtaggcga acgaccggcc
    13561 gttctggacg gagacggggc ccatcccgag ccccgcgaca agaaccctac gtccccgcca
    13621 ttaaagacgc gtaaagctta aaccgtcggg gcctgtgcga accggccgca acctcgatgc
    13681 gcgcggcggc caaagccgtg aaggggccaa tggattggcg tccggacgtg atcgcaacgt
    13741 tctgctaacc ataattagcg cgcctgtctc cggcggcggc actttcagcc ggccgacagg
    13801 ccggtgagtt cgaggctgcg cgcacgcaat cgctggcgcg cgcccgcatc ataggcctgc
    13861 ggattggcct gggcctgctg catgccgttg aaaaacagcc cgctcttgcc ggccacatcg
    13921 tcgccggcgg caagatggag tatcgcctcc ccgccctgct ccaccgtaga gatcggcgtg
    13981 atgccgcctt cgcggaccat cgtggtgttc atataggtcg cgggatgcag cgcattcacg
    14041 ctcacgcccg atccctcgag ttcctgggcg agatcgatcg tgaacatgat ctgcgccagc
    14101 ttgctctgcg cataggcgcg gttgccgtta tagcctttgg tcagcatcac gtcgtcgaaa
    14161 tcgagcgggt gctggccgag cgaggcgacg ttcacgatgc gggagggcgc gcttgccttt
    14221 agcaaaggca gcagcagatg tgcgagcaga aagccggaaa ggtaattcac ggcaaaccgc
    14281 agctcgtgtc cgtccgcact ggtttgtcgc ccagagccga gcgaccgcga tccgatcccg
    14341 gcattgctga tgaagacgtc gagccgctga tgcccggcga gcaccgcggc cgcgagttgc
    14401 cgcgctcctg caatcgacga caggtcggcc tgatagaaga ccggctcgat gtgtccctgg
    14461 ccgcggatct catcggcaag ggttttggcc cgcgcctggt cgcggccgtg gatcaggaca
    14521 ttggcgccgg ccgcggccag tctggcggcg acatagcggc cgacgccatc ggtcgagccc
    14581 gtgatcaaaa ccgtcttgcc ggccattttc atcgcaaagc gctcctacgc gtattccgca
    14641 aaagtgggta ccggttttgc gaatagaata cgcgcaaact aaagccgtca gtgaatttcg
    14701 gccgagcgag ccaaagcttc cttcagcttc tcaagggtga aggacgggct gcgggcgatc
    14761 tcgaggatcg gcgtttcgcg gcgaccgcag agttcggcgg cgtccggcag cttcgcggca
    14821 acatcgaacg gaatgaccac ggcgccgtgt cggtcggcat gaatgagatc gccggagcgc
    14881 accgtcattc cggccacccg cacctcgccg ccaaaacttt cggcatgaac ataggcgtga
    14941 gacggtccga tcgagcctgc cagcgcctga aagcccggcg cccattgcgg gatgtcgcgg
    15001 atcgatccgt cggtgatcac gccgacgcag ccgagcgcct tgtgcacggc actttgcacc
    15061 tcgccccaga acgcgccgta tcccacatcg gcgccgtcga tatcctgaat cacggtgatg
    15121 cgcggcccct gtccggtccc gacatattcg taataagcga gccgccgcgc ctgctgttcg
    15181 gcggcggcaa gtccggaagc caccacggag cggatcgtca ccgtgcgggc atagccgacg
    15241 atcggcggca gatcgggaaa cggacagacc agcggcctgg tcgtgtagcc gatcaggcga
    15301 cgtcccggcg cgacgatttc cagcgcgttg cagatcgtcg gcgtgtcatg gcgcgccaat
    15361 gcttcaagca gtgcagcagg cagcggcgcg gttgcggaat tcgtcacggc gttctctcca
    15421 gggttttgcg ggccggccag ttgcggaaag gccgtgtatg accagccata tagccgagaa
    15481 aagcgtgatc agcaaaatgc cgttcagttc agccgggccg gccgattatc ctgttcgggt
    15541 tcggtgacaa cagacagtgg cggcgccgga cttgaagggg cgacattggc cggcggcgtc
    15601 gccggcgcgg cggcattgcg cgcatggtcg cgataagact tccatccggc cggcaggctc
    15661 aaaaggtaaa gcaccgaacc cgccgatata atgtgccagg gatagctgac cagaagcgcg
    15721 atgaagaaaa tcaccgagac gaagaccggc agcaccattt ccggcggcac ccgcatgcgc
    15781 acggtcttgc ccgaaaacac cggcagccgc gaaaccatca ggaatgcaat cagcagcgtg
    15841 tacagcgccg tcagcatcgc cggcggcatc ggcaacccca ggaacgcaag gtagatcgga
    15901 agcagcacgg tgatcgcgcc ggcgggcgcc ggaacgccgg tgaaataatt cgccgcaaag
    15961 gccggcttgt tcggatcgtc catggtcgca ttgaagcgtg ccagccgcag accaccgctg
    16021 atcgcgaaaa ccattgccgc gatccagccg ccattgctca ggtcatgcaa ctgccagaaa
    16081 tacaggatca gccccggcgc gacgccgaaa ttgacgaaat ccgcgaggct gtcgagttcc
    16141 gccccgaatt tcgactggcc cttgatcatg cgcgccacgc ggccgtcgag gccatcgagc
    16201 acggcggcaa acacgatcgc cgccaccgcc aattccatcc gcccctcggt ggacagccgg
    16261 atcgcggtca atcccgcgca gatcgcgagc aaggtgatga cgttgggcac cagcatccgc
    16321 accggaatcg ggcggaaccg gcggcggcgc atttccggat atttgggatc aacgggcatc
    16381 tgcatggctc ggctatatat agcaagcgcc ggcctgctcc gccatcgcgg gcgtcaagtg
    16441 gctggttcgg cggcacaaat ggttaatcgg cgcggtaggt tcgaccggga tctccaagct
    16501 tgaaatcagc cagaatggtc tccccggcca ccgccgtctg gccttccgag accagcgccg
    16561 tggtgccttc gggcagatag acgtcgaggc gggatccgaa ccggatcaac ccgaaccgct
    16621 cgcccgcgcc gatcgtctgc ccttcgcgga cgaacgatac gatccggcgc gccaccaagc
    16681 ctgcgatctg gatcacgcca atgcgtccat tcggggtcga gatcacaagc gaattgcgct
    16741 cgttgtcctc gctggccttg tccagttcgg cattgataaa ggtgcccggc cgataggcga
    16801 tccggtcgat ccgccccgcg accggactgc ggttcacatg gcagttgaac acgctcatga
    16861 atatcgagac acgcggcagc ggcctgtcgc ccagaccgag ttcggccggc ggcagcacct
    16921 gcgtcaccat cgagatgcgt ccatcggccg gcgccacgac gatgccctcg cgtatcggcg
    16981 tgacccgcac cggatcgcgg aaaaaaagcg cgcaccagac cgtcagcaag gtgccgatcc
    17041 agcccagcgg cgtccagatc cagaacagga tcaggctggc aagcgcaaag ccgccgataa
    17101 acggatagcc ctccggatga acaggaggaa tttgcgcgcg gatggaattg gcgatggaca
    17161 tcagaattca ctgttcattt gcgccctctc ccgtctggga aaaggaatta cacttcgatc
    17221 attccgccgc ggctggagtc gccagcgcgt cgtcgaccag cggtggggcg cggttcggcg
    17281 cttcgttctc gtcggcgatc cgcgccagtc tctcccgcgc ctcctgggct tcacgctgcc
    17341 tgttccacat actggcatag agtccgccgg ttgccaaaag ctgggaatgg gttccacgct
    17401 cggcaatgcg gccctgatcc agcacgatga tttcgtcggc gccgacgatc gtggacagcc
    17461 gatgcgcgat caccagcgag gtgcggttgc gcgacacccg ctccagcgca tcctggattt
    17521 cctgctcggt atggctgtcg agcgccgatg tagcctcgtc gagcaccagg atcggcgggc
    17581 ccttgagaat tgtgcgcgcg atcgcgacac gctgcttctc gcctcccgat aatttcaggc
    17641 cgcgctcgcc tacttgcgtc tcatatccct tcggcgacat ccggataaaa tcgtcgattt
    17701 gcgcgagctg cgccgcctgc tccacctcgg cgtcgctggc gtcccagcgg ccataacgaa
    17761 tgttgtaacg gattgtatcg ttgaacaata cggtgtcctg cggcaccatc ccgatcgacg
    17821 cccgcagcga agattgcgtg acgttccgga tgtcctggcc gtcgaccagg atcttgccgc
    17881 ccgagatatc gtaaagccga aacagcagcc gcgagatggt cgacttgccg gcaccggagg
    17941 ggccgacgat cgcaacggtc ttgccggcgg gcacctcgaa gctgaggcct ttcaggatcg
    18001 gccgctcgga atcgtatgag aaccgcacat cgtcgaaacg aacactgcct gaggtcacga
    18061 tcaagggctt cgcgccggga acgtccttga cctcgggatt tcgtgacaga acgccgagca
    18121 tcttttcgat atcgatcacc gcctgcttga tctcgcgata gaccatgccc atgaaattga
    18181 gcggctggta aagctggatc atcatggcgt tgaccatgac gaaatcgccg acggtattgg
    18241 ttccgcggcg gacaccgatc gcgcacatca gcatggtcgc ggtcaatccg gcggtgaaaa
    18301 tgacggcctg accggtattg agaaccgcca gcgaggtata ggttttcacg ctggcgcgct
    18361 catagccttc catcgaacga tcgtagcgcg cggcttcgcg ctgctcggcg ctgaaatatt
    18421 tgaccgtctc gtagttcaaa agcgagtcga tcgccttggt gttcgcgtcg gtgtcggact
    18481 cgttcatctt gcggcgaatc tcgatccgcc attcggttgc gatgtaggtg tagtacatga
    18541 agaccacgac ggtgatcatg gtcgcgaaca cgtagcgcca gtcgaattgc cacagcagca
    18601 cgcccatcaa cagcgtgact tcgacgacgg tcgggacgag ttgcaggatc accatgcgga
    18661 caatgacttc gatgccgagc cggccgcgct ccagcacgcg cgtcaggcct ccggtcttgc
    18721 gctcgaggtg aaagcgcagc gacagctcgt gcatgtggac gaaggtgaga taggcaagct
    18781 tgcgcaccgc gtgcatcgca acccgggcga agattccgtc gcgccattgg gtcaggaccg
    18841 ccatcagcac gcgaatcgcg ccgtagctca aggtcatgat caggggcgac gcgatcagcc
    18901 acaacggcca gttcgatgcc tgcaccggcg cggtatcctt accggtcagg gcgtcgatcg
    18961 cccatttgaa ggtgaacggc accgagagcg tcgcgaactt ggcgaccagc aacagcacca
    19021 ccgaccagac cacgcgcatc ttgagatcga cccgatcgcc gggccagatg tatggccaca
    19081 gatgcgccag cgtcccgatc agggtcgcct gctcgaccga tcggccggaa gaaggctgcg
    19141 gttggccggc acccggggac gaatcagtgt aagtcattga tcaagagcct gaagaatcac
    19201 cggcgcacgg gtgttttgtg tcgatctgca tggtgtccgt catatagagc gtctgccctc
    19261 cgcttgcacc ctcacggagc aattctttcg ggatttgcag gcactttgcg gattaattca
    19321 actgccgtcg ccaattcaat gggcttgacg catttacgtt gcagtgccaa atccgagtat
    19381 gagcaagatc aaacccagca tgaacaagat caaaaccgtc tgtgtctatt gcggctccgg
    19441 cccgggaacc aatccccgct ttgtagaagc tgccatcgcg ctgggaaaga tcctcgccga
    19501 taacggcgtg agccttgtct atggcggcgg ctcgaatggc ctgatgggcg ctatcgccaa
    19561 atccaccctg gatcatggcg gcaacgtcat cggcattatt ccggagtttc tgatttcccg
    19621 cgaaaatgcc atggcgcgcg ttcaggaatt gatcgtgacg cccgacatgc acgaacgcaa
    19681 acggacgatg ttcgaacgct ccgatgcatt cgttgccctg cccggcggta tcggcacgct
    19741 ggaggaactg gtggagcaac tgacgtggca gcagcttggc cgccacgcca agccggtcct
    19801 gctcgccaac atcgatagct tctgggagcc cctgctcgcg ctgctcacgc acatgcgcgc
    19861 aacggaattc attcggccga ctctggcagt cgagattctg aaagcagagc acgtcgagga
    19921 tatcttgccg cggttgctcg ccgccgccgc cctcgccccc gagggcaccg acgaaatggc
    19981 cgccgaagtg atacgccagc tttagccatc gaagggttat cgcggcgaca cctctgcatc
    20041 gtctttgggg aacgtcaccg cttcgatgcg attgccatcg ggatcgatga cgaacgcggc
    20101 ataataacgc acgcgatcgt gcggacgcag acccggcgcg ccgtcggagc ggcctccggc
    20161 atgcagcgcg gccgcatgga atgcatcgac atcatcggcc gttttcgccc ggaggcagat
    20221 gtgggtcccg ctctcgggct gaacccgcgc catgccggca cgaaaattga tccagaactc
    20281 cggatagttt ttgccgaaac cgaccgttgc cggccgcgtc accagccgcg acagcccgag
    20341 cggcgccagt gccagttcat aaaagcgggc ggcgcgttcg aggtcactga cgccgacgga
    20401 aacatgatcg atcatctaag atgcatcccg aaagtagaag cgtcattccg gggcgcgcac
    20461 tagcgcgaag ccggaatctc gagattccgg atatggtccg gcggaccatt ccggaatgac
    20521 ggcgagtccg tcacgccggc gcgccggact tcaccagctt gtagatcacc gaatccatca
    20581 gcgcctggaa cgatgcgtcg atgatattgg gggagacgcc gatggtggtc cagcgcttgc
    20641 cctcctcgtc ttcgctttcg atcagcaccc gcgtcaccgc ctcggtgccg ccattgagga
    20701 tacgcacacg gtaatcgatc agcctcaggc cttcgatgta cttctggtat ttgccgaggt
    20761 ccttgcgcag cgccacgtcg agcgcattga ccggaccgtt gccttcggcc gccgagatca
    20821 gatgctcgcc ggcgacatcg accttcacca ccgccatcgc caccgtcacg cgctggccgt
    20881 tggcgttgta gcgctgctcg gtgttgacgt cgaactgctc gaccctgaaa tattccggca
    20941 cccggccgag cgtgcgccgc gccaacaggt cgaacgacgc gttggcggac tcgtaggcat
    21001 agcccgcggc ctcgcgctcc ttcagttcct cgaccaggcg aaccagtttc gagtcgtttt
    21061 tctcgtaagc gatgccggcg cgttccagtt cggcgatgac attggaacgg ccggcctgat
    21121 cggacaccag caccttgcga cgattgccga cagcttcagg cgccacatgc tcataggtct
    21181 gcggatcctt gagcacggcg gaggcgtgaa tcccggtctt ggtgacgaac gcgctttcgc
    21241 cgacataggg cgcgtggcgg ttcggtgccc gattgagcat gtcgtccagc gtccgcgaaa
    21301 ccttcatcaa ggtcgccatc ttgtccggcg acacgccgat gtcgaagcaa tcggaaaact
    21361 cgcgcttgag ccgcagcgtc gggatcagcg aacacagatt ggcgttgccg cagcgctcac
    21421 caagcccgtt cagcgtgccc tggatttgcc gcacccccgc gcgtaccgcg gccagcgaat
    21481 tggcgacggc ctgttcggtg tcgttatggg cgtgaatgcc gacatgatcg ccgggaatgt
    21541 gtttcagcac ctcagtgacg atggcctcga cctcgtgcgg catggtgccg ccgttggtgt
    21601 cgcacagcac cacccagcgc gcgccggaat cataggctgc cttcgcgcag gaaagcgcga
    21661 aggcggcgtt ttccttgaaa ccgtcgaaga aatgctcgca gtcgagcatg acttcgcgcc
    21721 ccgccgactt cgcggccttc acgctgtcgc ggatcgacgc cagattttca tcgttggtgg
    21781 tctcgagcgc gacgcgcacc tgatacgccg atgatttcgc gacaaaacag atcgcatccg
    21841 ccctggcctc cagcagcgcc gcgagacccg ggtcgttcga gaccgaacgg ccgggacgcc
    21901 gcgtcatgcc gaacgccgtg aaacgggcat gagcgagatc aggcttttcg gcaaagaatt
    21961 cggtatcggt cggattggcg ccgggatagc cgccctcgac ataatcgata ccgagctcgt
    22021 cgagcatctt tgcgatcagc tgcttgtcat gcagcgtgaa atcgacgccg ttggtctgcg
    22081 ccccatcgcg cagcgtggtg tcgaacagat aaaggcgttc gcgagtcatg tcggaactcc
    22141 tgacgacggg ccctggacaa gcgtcttctg catcgtggtg ttggcaagcc attcgccgtt
    22201 gacggtcacg gtgttgcgct gcttgccgac gtaaccgcgc tttaggaaaa attcctgcgc
    22261 attgtcgctg gcgtcgaccg agagactcct ggcgccgcgg ctgccggcga gcttttccag
    22321 tgcgtcacac agcatcgacg ccacgccctg cccgaccgcg cccggatgca cataaagcat
    22381 gtcgatatga tctgcgccct tcagcgaggc aaatccgacc ggagaattct gcagcgtggc
    22441 gatcagcgtg agttcggagg cgaggcgctt gccgaattgc tcttcgtcat cggcggcgct
    22501 ggcccaggcc tgctgctgcg cttcgctgta gtcgtcgccg gtcaactcct cgacgcttgc
    22561 gacgaagatc gccgccagca tcggcgtatc cgcggcgagg aacggccgta aagcgggttt
    22621 gggcagtgcc tgtcccatta cacgatctcg tccaggaaga gatttcgcat tggctgccaa
    22681 cagccgcgtc cgtcatgcgc gggctcgacc cgcgcatccg tccaaaccac ggcagcagaa
    22741 ttttttaatt gatggattgc cgggtcgagc ccggcaatga cgccgatttc ggaggggttc
    22801 atcgcgcgat ctcccaggtg gtgccttcct tggaatcctt gatgactacg cccatcgccg
    22861 cgagttcgtc acggattcga tcggattcct ggaagtcttt tcgcgcccgt gcggccgcgc
    22921 gatctgaaat caaaccatcg atttgtttga cgtcgacgcc gctcgcttgc tgttttcgac
    22981 ctttccattc cgccgcgctc tcggacaaga aacccagaag ccgcaaggaa gcggcaaacg
    23041 cgttgcgatc ccgttcactt ccggaagcgg cgctgttgcg caaaccgtgc agcgaggcga
    23101 tcatctgcgg cgtattcagg tcgtctgaga gcgcctcgat aactgcctca gaaggccgtt
    23161 cgcccttgtt gtccgcagcg acccagtacc agtcgtcgag cgtcttcgcg ctttcttcca
    23221 gtcccttcga cgtccagtcc atcggagagc gataatgcgc cttcagcata ttgaggcgta
    23281 acacttcgcc cggccaatcg gccagcaatt cccggatcgt gacgaagttg ccgagcgact
    23341 tcgacatctt cgcgtcttcg acttgcacga attcattgtg catccaatag ttcgccatgg
    23401 caggcgtacc atgcgcgcat cgcgactgcg cgatttcgtt ctcgtggtgc ggaaagatca
    23461 ggtcgatgcc gccgccatga atatcgaaga cctcgccgag ataggcggcg ctcatggccg
    23521 agcactcgat gtgccagccc ggacgtcccc tgccccatgg gctttcccat ccgggctcat
    23581 cgccggacga ttgcttccac aacacgaaat cgagcggact ctttttatgc gcatcgacgg
    23641 caacgcgcgc gcccggccgc tgctcgtcga gcttgcggcc cgacagcgcg ccataatcgg
    23701 gcatcgactg gacatcgaac agaacttcgc cgccagcttc ataggcatgg ccgcgcgcaa
    23761 tcaattgctt gatcaaggtc accatgtccg ccttgccgtc ggcgcgcggc agcacgaaat
    23821 cggtggcgcg cggttcgaac gttggcggca aggagcccag ggccgccaca tctgaagcga
    23881 attgtgccgc cgtcttctcg gtgactttcc ggatcgcctc gttcagcggc aggccgggga
    23941 aatcacgcac ggcgcgatcg ttgatcttgt cgtcgacgtc ggtgatgttg cggacataag
    24001 tgacgtggtt ctcgccgtag atgtgccgca gcaggcggaa cagtacgtcg aacacgatga
    24061 ccgggcgggc gttgccgata tgggcgaagt catagaccgt cggcccgcag acatacatgc
    24121 gcacgttcgc aggatcgagc ggcacgaaga tgcgcttttc cctggtcaag gtatcgtaga
    24181 ggcgtaagtc catggatatc ccgtcccttg cggccgggcg tccagtgatc tcgaatgaga
    24241 aaagacggcc tcagccagcg aatcgctagc tcataatctc gcggcaaatg ccacaaatgg
    24301 cgaggagacc gttcatggcg ctaccatggg ccagcccggg ggacggcgtc aagagcttgc
    24361 taatgacgga ttttcgccat caatccgccc cggctgccat ggttaatcat tattaacgtt
    24421 ttgcgtttat gcaatgaggg gcggtcccct cattttttta tggtgcagca tgcgatccct
    24481 ccctgcgctt ttggcctgta cgttcctcct cggcgcgtcc gcggcatcgg ctgacagccg
    24541 cgttttcatc atcgccaacc aggccgacgg ttacggcgtc gaccagtgcc tcgcgagggg
    24601 cgaccaatgt ggcgcctacg cggcccggtc ctattgccag tcgcgggatt tcacgcaggc
    24661 ctcggcctac cggcgcgtgg atccggatga agtgacgggg tcggttccca aacctgccgg
    24721 tggcagctgc aaccatgccg gctgcgatga atacgtcgcc attacctgtc aacgctaaag
    24781 ccatcgtcgc ggcgatcgaa gggcgggccc gccacaccgg cgcctcggaa acgacgtgac
    24841 ggtgccccgc caagccggta tgaccggttt tccgaaaaga ccatgcgcaa acaaagagat
    24901 cgcatcatga tccgatctaa tctaatcgga tcatgatgag atggccggat atgcctgaaa
    24961 cgctgattac cccctttgcc agatggattt ttgcctgtgc tgcgctgttc ggcgcggccg
    25021 tgctgagcgt tgacgcttcg gcgcaaatga gtcaggcgcc cccgccgccg cagcagcagg
    25081 gagcgcccgt caatccgatg tgcccgcggc tggaggcgca attggcgtcc atcgaccgcg
    25141 gcggcgggga tccggccaag gacgagcaaa tccgccgcta tcaggatgcc gcgaccaagc
    25201 agcagggcga gctcgaccgc gttacctcgc aggccaagcg catgggctgc gacagttcgg
    25261 gatttttctc gctgttcagc ggtcaatccg cgcaatgcgg tccggtcaac acccagattc
    25321 agcagatgcg cgccaacctc gaacagatca cgaccagcct cgagcggctg cgtaccggcg
    25381 gcgtcggcgg cgccgatcgg gacaatcagc gccgttcggt cctgaccgcg ctcgcgcaga
    25441 acaattgcgg accgcaatat gccaacgccg cgcggggctc gggcaatttc ctggagaatc
    25501 tgttcggcaa caacaataac aatccggtgg cgccgcccgg cgccgatctg ggtgcgcaat
    25561 ccgggaccta tcgcacggtc tgcgtccgca gctgcgacgg cgcttatttt ccgatctcgt
    25621 tcgcgaccgt gcccggtcgt ttccccgacg acgaaaggac ctgcaaggcg ctttgcccgg
    25681 cggcggaggc aaccctctac agctaccgca atcccggcga agacatgaac caggcgatgt
    25741 cgatcaacgg ccagccctat tcgtcgtcgc ccaatgcgtt tcgctaccgt caggagttca
    25801 atccgtcctg cgcctgcaag gccgccggac agacctggtc cgacgcattg aagtccatcg
    25861 acgacaaggc cgaggccgaa caacaaggcg acatcatcgt cacagaagag agcgccaaga
    25921 agatggcccg gccgctgacc aaaggggctg caaccaagaa gggcgccgcg gccacgacag
    25981 cgaccgcgcc ggcacctgaa gcatcgcccc ccgccgccga ttcgaccggc gcagcaccag
    26041 acaacaagcc aattcgctcg gtcggcccga cctttatccc ggcgcgttaa tccggttgat
    26101 ctacccctcg aacgcttccg ccgacccgcg gctggcgcgc gagaccaggg tatcgtcggg
    26161 ttccggcagc gcattggcca gatcgcgaaa cttgttggtg atgggataac ggcggtcgcg
    26221 cccaaagttc ttctgggtga ccttgacgcc aggggccgcc tggcgccgct tgtattcggc
    26281 gatgttgagc aggcgatcga tccgcgccac cacttcacga tcgaaccccg ccgccacgat
    26341 cgaggccagc ggctcttcgc gctccaccag acgttccagg atcgcatcga gcatgtcgta
    26401 gggcggcagc gaatcctgat cggtctggtt ttcgcgcagt tccgcggtcg gcggccgcgt
    26461 gatgatgttg accggaatga cttcgcccga cgggccgagc gcgccgtccg gcttccacgc
    26521 attgcgcagg gaagagagcc ggaacacctc ggttttgtag atgtccttga tcggattgta
    26581 gccaccgttc atgtcgccat agagcgtggc gtagccgacc gacatttccg acttgttgcc
    26641 ggtggtcacc accatcgcgc cggtcttgtt ggagatcgcc atcagaaacg tgccgcgggt
    26701 gcgcgcctgc aggttctctt cggtgatatc gcgcggcaga ccggcgaagg ttttggacaa
    26761 gatctcttca aagccgttca cggcctgcgc gatcggcagc acctcgtagc ggatgccgag
    26821 cgcgagtgct agttttgccg cgtcatcgag cgacacttgt gcggtgaaac ggaacggcag
    26881 catgacgccg cggacctgcg cagcgccgag cgcatcgacc gcgatcgccg cgcagagcgc
    26941 cgaatcgatg ccgccggaga cgcccagcag cacgcccgga aatccgttct tccggacata
    27001 gtcgcgcagt cccagcacgc acgcggcgta gtcgcccttg tcgccctcga cgagcggcgc
    27061 catcggtccg gtgcagcgcc agccggcttc gcctttgctc caacgcagcg tcgtgatgtt
    27121 ttcctcgaaa gccggcaatt gcgccgccac cgacagatcc gcattcagcg caaacgacgc
    27181 gccgtcgaac acaagctcgt cctgcccgcc gatctgattg agatagatta gcggcagccc
    27241 gctttcggtg acccgcgcca ccgcgatcga cagccgcaaa tcgttcttgt cgcgcgcata
    27301 gggcgagccg ttcggcacga cgagaatttc cgcgccggtc tcggcgagac attcgacgac
    27361 gttctcgtaa tcttccgact cctcgagcca gatatcctcg caaatgggaa caccgacccg
    27421 gacacccctg atggtgaccg gaccggcggc cggaccgcgc gcaaacaggc gcttttcatc
    27481 gaatacgccg tagttcggca ggttggcctt gaaacggatg gccgcgatgc gtccctcgtc
    27541 cagcagcgcg caggcattgt agagcttgcc atcctcaacc cagggactgc cgatcaggac
    27601 agccggcccg ccatcggcgg tgtcgcgcgc cagttcctcg acggcggcgc ggcacgccgc
    27661 ctggaacgcc ggcttcaaca ccaagtcttc cggcggatag cccgtaatga acaattcggg
    27721 caacaccagg agatcggcgc cgtccaagcg cgcctgcgcg cgcgcggcgc gcgccttggc
    27781 ggcgttgccc gcgacatcgc cgaccgtcgg gttcaactgg cccagggtga tcgcaaactg
    27841 ggcggcacgt tcggtcatgg cgcgaaccta cttatcctca taatgagcaa attaaatata
    27901 gcatgccccc tcgggcttcg ccagatgcca attgcatgat ttagaccgcg cccatgcgtt
    27961 cggcgatggc gaacagccag aacaggccgg ccatgacaag tgcaacgccc actgccgccg
    28021 aacccatgtc cttgacccgc ccgatctgcg gatcatgttc ggtggtcagc cggtcggcga
    28081 gcttctcgat cgcggtgttg agcaattcga ccaccagcac cagaaccacc accgcgatca
    28141 gctcgacgcg gcgcatcgtg gtggcgccga tcagccaggc cagcggcaac gccagcgcca
    28201 gcgcggtcag ctcctcgcga acggcctgct cggagcggat ggcaaagacg aggccgttgc
    28261 gcgtattgat cgtggcccgc cagaaccgca gcaactcaga gtcccgcggc gacaggcatc
    28321 ggcttggccc tgccgccgcg tcccttgagc aattccgcga tcaggaaagc catgtcgatc
    28381 gactgctcgg cgttgaggcg ggggtcgcag gccgtgtgat aacgatcgct gaggtcctca
    28441 tgggtgatgg cgcgcgcgcc gccgatgcac tcggtgacgt cctgcccggt catctccaga
    28501 tgcacaccgc cggcatagct gccctccgcc gcgtggatgg agaagaacga cttcacctcc
    28561 gacaagatcc ggtcgaacgg acgggtcttg tagccgctgt tcgaggagat ggtgttgcca
    28621 tgcatcggat cgcacgacca caccaccacc cggccttcgc gctgcacggc gcgaatgagt
    28681 tgcggcagat gatcgccgat cttgtccgaa ccgaagcgat tgatcagcgt caggcgtccc
    28741 ggatcattgt ccggattgag cacgtcgatc agccgcagca attcgtccgg cttgagcgag
    28801 ggaccgcatt tcaatccgat cggattcttg atgccgcgga aatattcgac atggccgtga
    28861 tcgagctggc gggtgcggtc gccgatccag atcatatggc ccgacgtcgc atagtagccg
    28921 ccgctggtgg aatcgacccg ggtgaaggcc tgctcgtagc cgagcagcag cgcctcgtgg
    28981 ctggtgtaga aatcggtggt gccgaggctt gaatcgaccg cgaagttcag gccgcaggcg
    29041 cgcatgaaat ccagcgcctc cgaaatccgg tcggccaggt ccatgtagcg cttcgactgc
    29101 ggcgaatcct ttaccgattc gagcatccac tgatgaacat tgtcgaccga ggcataaccg
    29161 cccttggcga aggcgcgcag caggttcagc gtcgccgccg attgccgata ggcttcgatc
    29221 tgccgccgcg gatccggcgt gcgcgcctgc gccgtgaagg cgatgtcgtt gacgatgtcg
    29281 ccgcgatagc tcggcagttc gacgccatcg atggtttcga tcggcgacga gcgcggcttg
    29341 gcgaattgac cggcgacgcg gccgatcttc accaccggca gcgcgccggc ataggtcagc
    29401 acgatcgcca tctgcaggaa gacgcgaaag taatccctga tattgttggc gccgtgctcg
    29461 gcgaaacttt cggcgcaatc accgccttga agcaggaagg cgtcacctgc ggctacccgc
    29521 gcaagcgccc ttttcaggtt gcgggcctca cctgcaaaaa ccagcggcgg aaacgtggca
    29581 agctgcgcct cgacatcggc caatgccttg gcatcgggat agtcgggtac ctgcttgacc
    29641 ggcctggcgc gccagctgtc gggtgtccac cgctcggaca tgaccttaac tcctgagcaa
    29701 aaaccgctac ttatcacggg tagtgagggc cggtttatac acaggctgat gcccgaccgc
    29761 cagttggatt tctggtctgc gagcgaagcc cttgcggcaa tagccgaatt cgcatttgtt
    29821 gcgcagctta gcgaaggccc gggggataac gcggtggcag cgactgcgct ggacgacttt
    29881 ttttccgatg acatcacctt ccccgccatg gacggatatc tcttgggggc gacgctgttt
    29941 ttgccgctcg gcgccaaacg tcatgccgtc ctgatcaatt cggcgaccgc ggtgccgcgc
    30001 aggatctacc ggggttttgc cggctatctg gcgcggcggg gctgcgcggt cctgacctac
    30061 gactatcgcg gcaccggcga cagccggaag aaatcgccgg ccggctcgcg tcggccgcga
    30121 tcgctggccg gcttcaaggc cggcatgtcg gactgggcgg cgctcgatgt gaccgccgcg
    30181 gtggcctgga tgcgcgagcg ttaccaccat ctgccgctca attatgtcgg ccactccttc
    30241 ggcggccagg cgctcggact gcttcccaac aacagcgagg tttcgcgcgc gctcttgatt
    30301 gcggcgcagg ccggatactg gaagctgatg gccgcgccgg agcgctaccg cgtctatgtc
    30361 atgctgaatt acgtcggcac tcccctcacc agggtattgg gctatgcgcc gggctggagc
    30421 ggcctcggag aggatctgcc aaggggcgtg tttcagcaat gggtgggctg ggtcatgagc
    30481 cggcgctatc tgttcgacga caccaatctc aaggcgctgc agaactttcc gcaattccgc
    30541 ggggcgatgc gcgcgctgtg tttttccgac gatccctggg cgacgccgcc tgcggttgaa
    30601 ctcctgtgct cgggcttcat cgccatcgat ccggaaatcc tgaccattgc gcccgccgac
    30661 gtcggcgcca agcggatcgg ccatttcggc tttttccgcc ccgaacaccg cgacacgctg
    30721 tggcgaggag cggcggaatg gattcaggca gcggaatagg ctcgccctcc ccgtcgtccc
    30781 ggccttgaga ttcaaacggc tgcggtccta ttccctcccc ccttgcgggg gagagagagc
    30841 gctgccgctg gcgcaagttc tcgattcaat tttcaaacag ccaagaccgt cattgcgagc
    30901 gaagcgaagc aatccatggc cgtaacaaaa agaaagagtg gattgcttcg cttcgctcgc
    30961 aatgacgatc tctagacatg cgtttaaatc ccgcgacgcg atgcgcccga gctgtgtatg
    31021 aatcttccgc ccaaggaggg cgtggggaat gccggatgcc cgctgcaccc gcggcctcgt
    31081 gtgcaattat agtggtagaa cgcacacgag taacaacgag tacaccggaa tcacccggca
    31141 ttcccgcacg caatggtttt aacggattcc ttcgcgctct ccccggtgac cgggctcttt
    31201 tgccaccgtc gtcagcggat caagttttgt ctaagcccgg ttgggccgac ttaactccgc
    31261 gaacttgacg ccagcgtcgg ggcgtcagga ccacacgact tcgccgtacg caacatcgtc
    31321 tctcgtcagc gtgctgttga tcgctcacaa gcctttcgac ca
//
LOCUS       DATNOI010000296         3380 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_596150,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000296 DATNOI010000000
VERSION     DATNOI010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596150"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VN968_11640"
     CDS             complement(<1..215)
                     /locus_tag="VN968_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29926.1"
                     /translation="MPNDTRELPAANDGLYRFLGGSPLTVAFRLILLSILVGVVLAAI
                     GFDPWNIVNSIRLLFQRIWDLGFDAING"
     gene            complement(391..2208)
                     /locus_tag="VN968_11645"
     CDS             complement(391..2208)
                     /locus_tag="VN968_11645"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXL29927.1"
                     /translation="MNRFAELLDRLAYEPGRNNKLRLLTSYFREVEDPDRGYALAALT
                     GALSFKHAKPGLIRDLIASRTDPVLFALSYDYVGDLSETVALMWPKSSPSPACGGGPG
                     WGQAASAVPEREPPPGSPDSAKALPSVPTSPASGRGKKDTRHVSNLHNNPPPPTLTEV
                     VTTLRTLGKTELPKQLTCWLDELDETGRWALLKLVTGAMRIGISARLAKTAAAALGDK
                     DPHDVELMWPGLAPPYLDLFAWLEGRADKPVNRDPVPFRPVMLAHAIENTDFSNLDAA
                     DFIAEWKWDGIRVQAVSGRDERGHIVARLYSRSGEDITKSFPDLLPSLHLPGAIDGEL
                     LVLRDGRVQTFNVLQQRLNRKVVSPKLIKEYPIHLRAYDLLSEGDTDLRTLPFAERRK
                     RLEAFVRRLDDARVDLSPTITFDSWDALTAARKNPASAGAGADSEAVEGVMLKRREAA
                     YLPGRPKGQWWKWKRDPHIIDAVLMYAQRGHGKRSSYYSDYTFGVWTSGEDGEQLVPV
                     GKAYFGFTDEELLQIDRFVRRNTVEKFGPVRHVVHEPDQGLVLEVAFEGLARSPRHKS
                     GVAMRFPRINRLRWDKPPREADRLETLERMLKADAVLPA"
     gene            complement(2205..3245)
                     /locus_tag="VN968_11650"
     CDS             complement(2205..3245)
                     /locus_tag="VN968_11650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HXL29928.1"
                     /translation="MRAQDILMPVPAGLCCKPGGFHIDPVRPVERAVITHGHSDHARP
                     GHGAVLATRETLDMMRLRYGDNFARSTQAIAYGEEIRLGDVTVRFHPAGHVLGSAQIA
                     VSRKDTCIVASGDYKDAPDPTCAPFEVVRCDVFITEATFGLPVFRHGNAADEIKKLLA
                     SVALFPERAHLVGAYSLGKAQRVIALLRLAGYDAPIYLHGAMEKITRYYESRGVALGD
                     LRMVKGTKKADLAGTITLAPPSATSDVWTRRFPDPVTAFASGWMRVRARARQRGVELP
                     LVISDHADWDGLTATINATGAGEVWVTHGQEDALVHWCTARGLTARPLDLVGYGDEDD
                     SDAAPADEDAEA"
BASE COUNT          584 a         1083 c         1085 g          628 t
ORIGIN      
        1 ccgttgatgg cgtcaaagcc gagatcccag atccgctgaa acaaaagccg gatgctgttg
       61 acgatattcc aggggtcgaa gccaatagcg gcaagcacca ccccgaccag gatcgacagc
      121 aggatcagtc gaaatgccac cgtcagcggc gagcctccga gaaagcggta caggccatcg
      181 ttggccgccg gcaattcccg tgtatcgttt ggcattcgat gatctcccgc accaattcgc
      241 ttgggcacta tagtcagtgt tggggagatg gggaacccgc atctcggcgt caatggccgc
      301 cagccgcggc ttgttatttt gacacgtttt cttcacgcga accggtgtcc accccggatc
      361 aagtccgagg gcatgcctcg ctggaaaatg ctacgcgggc agaaccgcat ccgctttcag
      421 catccgctcc agggtttcca gcctgtcggc ctcgcgtggc ggcttgtccc agcgcagccg
      481 gttgatgcgc gggaaccgca tcgcaacgcc ggatttatgc cgcggcgagc gtgccaggcc
      541 ttcgaaggcg acctccagca ccagtccctg atccggctca tgcaccacat gccggaccgg
      601 cccgaatttt tcgactgtgt tgcggcgcac gaagcgatcg atctgcaaca gctcctcgtc
      661 ggtgaagccg aaataggcct tgccgaccgg caccaattgc tcgccatcct cgccggaggt
      721 ccagaccccg aacgtatagt ccgaataata ggacgaacgc ttgccgtggc cgcgctgcgc
      781 atacatcaga acggcatcga tgatgtgcgg gtcgcgcttc cacttccacc attgtccctt
      841 ggggcgcccc ggcagatagg cggcctcgcg gcgcttgagc atcacgcctt cgaccgcctc
      901 ggaatctgcg cccgcgccgg cgctggcggg attttttcgc gccgcggtaa gcgcgtccca
      961 gctgtcgaag gtgatcgtcg gcgacaggtc gaccctggca tcgtcgagcc ttctgacaaa
     1021 agcctcgagc cgcttccggc gctccgcaaa cggtagcgtg cgcagatcgg tatcgccttc
     1081 gcttagcaga tcataggcgc gcaggtggat cggatattcc ttgatcagct ttggcgagac
     1141 cactttgcga ttgaggcgtt gctgcagcac gttgaaggtc tgcacgcggc cgtcacgcag
     1201 caccaatagc tcgccgtcaa tggcgccggg caaatgcagc gacggcagca ggtcgggaaa
     1261 gcttttggtg atgtcttcgc cgctgcgtga gtaaagccgc gcgacgatat ggccgcgttc
     1321 gtcacggcca ctgaccgcct gcacccggat gccgtcccat ttccattcgg cgatgaagtc
     1381 ggcggcgtcg agattagaaa aatccgtgtt ctcgatcgca tgcgccagca tcacggggcg
     1441 gaacggaacc gggtcgcgat tgaccggttt gtcggcgcgg ccttccagcc aggcgaacag
     1501 atcgagatag ggcggcgcga gcccgggcca catcagttcg acatcgtgcg ggtccttgtc
     1561 gccgagtgcc gcggcggcgg tcttcgccag ccgcgccgag atcccgatcc gcatcgcgcc
     1621 ggtgaccagt ttcaacagcg cccagcggcc ggtctcgtcc agttcgtcga gccagcaagt
     1681 gagctgcttc gggagttcgg tcttgccgag cgtgcgcagt gtggtcacga cttcggtgag
     1741 ggtagggggt ggcgggttgt tgtgcaggtt tgatacgtgg cgcgtatctt tcttacctct
     1801 cccgcttgcg ggggaggtcg gaacgctcgg cagcgcttta gcgctgtcgg gcgatccggg
     1861 tgggggctct ctctcgggca ccgccgacgc tgcttgcccc caccccggcc ctcccccgca
     1921 agcgggagag ggggaagact tcggccacat cagcgcaacg gtctcggaga gatcgccgac
     1981 gtaatcatac gacaacgcga acagcaccgg atcggtgcgc gatgcgatca ggtcgcggat
     2041 cagcccgggt tttgcgtgct tgaacgacaa tgcgcccgtc aacgccgcca gcgcgtagcc
     2101 gcgatcggga tcttcgacct cgcgaaaata ggaggtcaac agccgtagct tgttgttgcg
     2161 gccgggctcg taggcgaggc gatcgagcag ttcggcgaag cggttcatgc ctcggcgtcc
     2221 tcgtctgcgg gcgccgcatc gctgtcgtcc tcatcgccgt aaccgacgag atcgagcggc
     2281 cgcgcggtca ggcccctggc cgtgcaccaa tgcaccagcg cgtcttcctg gccatgggtg
     2341 acccagactt cgcccgcacc agtggcgttg atggtcgcgg tcagtccgtc ccagtcggcg
     2401 tggtccgaaa tcaccagcgg cagttcgacg ccgcgctgcc gcgcgcgggc gcgcacccgc
     2461 atccagcccg atgcaaacgc cgtgaccgga tccggaaatc gtcgcgtcca gacatccgat
     2521 gtggccgagg gcggcgccag cgtgatggtg ccggccagat cggccttctt cgtgcccttg
     2581 accatgcgca ggtcgcccag cgccacgccg cggctctcat aatagcgcgt gatcttttcc
     2641 atcgcgccgt gcagatagat cggtgcgtcg tagccggcca agcgaagcag cgcgatcacg
     2701 cgttgcgcct tgccgagcga gtaggcgccg accagatgcg cgcgctccgg gaacagcgcg
     2761 accgaagcca gcagcttttt gatttcatcc gccgcattgc cgtgacgaaa caccggcagg
     2821 ccgaaagtgg cttcggtgat gaatacatcg caacgaacga cttcgaacgg cgcgcaggtg
     2881 ggatcgggcg cgtccttgta atcgcctgac gcgacgatgc aggtgtcctt gcgggatacc
     2941 gcgatctgcg ccgagcccag cacatggccg gcggggtgaa atctgacggt gacgtcgccg
     3001 aggcgaattt cctcgccata ggcgatggcc tgggtgctgc gggcgaaatt atccccgtag
     3061 cgcagccgca tcatgtcgag cgtctcccgg gttgccagca ccgcgccatg gcccggtcgc
     3121 gcgtggtcgg aatggccgtg ggtgatcacg gcgcgctcga cggggcgcac cggatcgata
     3181 tggaagcccc cgggcttgca gcaaagcccg gcgggaacgg gcatcaggat gtcttgcgca
     3241 cgcatcttgg gtatataggg ctgcgtgaga ttcattcgag tctcctcccg cctgctcttg
     3301 ccgccaacct gccttttgat ccgtggtcga ttccgacgat gcccgtacgt ttgttcctgt
     3361 cctccggcga tctgatggcc
//
LOCUS       DATNOI010000297        13825 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1454648,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000297 DATNOI010000000
VERSION     DATNOI010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13825
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454648"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="VN968_11655"
     CDS             complement(<1..1387)
                     /locus_tag="VN968_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HXL29929.1"
                     /translation="MAERISSVKVSVGFASETGPRKRNEDFAGAVFGPELPEPRRDVA
                     AAISDGIGSTKGGREAAEIAVRGFLDGFCDLPETMEVRRAAATVLNALNGWIYSEGRR
                     DTRLTGMGCTFTALVLRGRVAHVLHVGDTRAYRLSRDRLTCLTSDHVRPGGAGRSNIL
                     TRALGVETEVRLDYTNQPVAQHDRFLLCSDGVHAYLAAETIADIMRERVSSEDTARAL
                     VAAALQSDSSDNCTALVLDVVGLPTAESAEIGANIAQLPIIPVPLGGETIDGFVLKVL
                     LSDGRYSRLFGAEDEVEGGEVALKFPKPQVASVAAYRAAFVREAWVGARVTSPWLGHI
                     IELPPGRQSCLYTVMPLYQGELLETRLTRGPGVGLEEGRHIAIRLARAAAALHRAGIV
                     HRDIKPDNVILEGEESLKLIDLGVVRIPGMEDFPPEEIPGTPAYMAPEMLDGEPGNEA
                     TDIYALGVTMFR"
     gene            complement(1487..1765)
                     /locus_tag="VN968_11660"
     CDS             complement(1487..1765)
                     /locus_tag="VN968_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29930.1"
                     /translation="MAGPAFSGEAARGETAVTCTNVASGTNWQIRIDYDKATVDANPA
                     RISDAEISWHEPADGGNYTLDRKSEKLTVIVASSTGGYFLYHRCRLQN"
     gene            2033..3355
                     /locus_tag="VN968_11665"
     CDS             2033..3355
                     /locus_tag="VN968_11665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HXL29931.1"
                     /translation="MALVPSPQWLDSGNNAWQLAAATFVGLQSIPGLTVLYGGIVKKK
                     WAINSAFMAFYAFASVLVVWVLFDYNMAFGPQWFPFLGTPGLATSATFTTGQAIIPAA
                     ASGMPALTFPMATLIFFQFVFAAITVIILAGSVLGRMNFTAWMIFCPVWMTLVYTVGA
                     FSIWGGGWLAAMGVADFSGGYVIHLAAGTSGFVAAWLVGPRLQADRDHFPPNSLLMTL
                     VGAGILWLGWNGFNGGDPYFANANAGAAVLNTNTCTAVALLVWTLMDKMAYGKPSVLG
                     AVNGMIAGLVAITPGAGYVDGVGAIMIGIVAGIIPWLAMNKLQKASFMMKVDDTLSVL
                     STHGVAGLTGGLLTGILANPAMLLYIGTDKEAPGVNVTGLLYGNAPQLLLQAEGAAFI
                     IVYNVIMTFIILKVISFIVPLRMDEATLKVGDDAVHGETAYAIGVEGE"
     gene            3757..5082
                     /locus_tag="VN968_11670"
     CDS             3757..5082
                     /locus_tag="VN968_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL29932.1"
                     /translation="MAMAITHATLSASDHSAQLRKAVIASTIGTAIEWYDFFLYGTAA
                     GLIFGKLYFPNEDALTATLAAFGTYFIGFVGRPIGAAIFGHYGDRIGRKATLIATLLC
                     MGIATFLIAFVPTYASIGIWGAVILTVLRMLQGIGVGGEWGGSVLLAMEWSRTHGQRG
                     LVASWPQFGVPCGLFLANLAVLAFSQLSGDQFSTWGWRIPFALSIILVGVGLWIRLGI
                     LETPVFQQLLDTRKIEKTPIIEVFKKHPKEIFLSALLRMSEQAPFYIFTAFIFAYAVG
                     TLHMSRDLILIAVLVASCVSFVTIPLSGHISDRIGRRKMYLIGAATVGVFGFLYFGMV
                     DTAIPALVFIAIVLSLVPHDMQYGPQAALIAEAFTPRLRYSGASLGYQLASVIAGGPA
                     PLIATALFAAYDSGYAISVYIAACSVVSLVAAWFMPDYTGRDISKEYDD"
     gene            5336..5488
                     /locus_tag="VN968_11675"
     CDS             5336..5488
                     /locus_tag="VN968_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29933.1"
                     /translation="MHCNDADQILTGGKDDRDETVAIGDRLRHVVKKTLFLLTQGVAI
                     DPLFEM"
     gene            complement(5639..6964)
                     /locus_tag="VN968_11680"
     CDS             complement(5639..6964)
                     /locus_tag="VN968_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HXL29934.1"
                     /translation="MAATIATDARAGAARPWYKVLYLQVLIAILLGVLVGWLAPTFAT
                     NDWIKALGDGFIKLIKMVIAPIIFCTVVSGISHIQDARKVGRVGVKALVYFEIVSSFA
                     LIIGLVVGNLIPVGRGLAVNPDAAAVATYVKQAEAQKSVDFVLNIIPDSVVGALARGD
                     ILQVLLFAILFGFALMALGERGHRLREVIDDTAHAVFGVIAIVMKAAPLGAFGAMAYT
                     IGKFGPAALGNLVGLIAVFYMTAALFVFGVLGLIARLVGFNIFKFLAYIKDELLIVLG
                     TSSSESALPQLMAKLERLGCSKPVVGLVVPTGYSFNLDGTNIYMTLATLFIAQALGVD
                     LSFSQQVTILIVAMLTSKGASGVTGAGFITLAATLTVVNPALVPGMAIVFSVDKFMSE
                     VRALTNIIGNGVATVFVSWWEGELDHDRLHAHLNKKIDPSDVETAVTTG"
     gene            complement(7091..7651)
                     /locus_tag="VN968_11685"
     CDS             complement(7091..7651)
                     /locus_tag="VN968_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HXL29935.1"
                     /translation="MLWPDRDEGVSGVLRIVILIALGTALLTLSAKVNLPLPYVPMTL
                     QTLVVLMIGAAYGARLGGATVTAYLAMGAIGWPVFASAVGGLAPLVGPTAGYLFGFVV
                     AAFITGWLSERGWDRSVARLFVAMGLGHIVILAAGFAWLAFGMKLGVEKAWLVGIAPF
                     FAASVIKNALGAALVPAIRHVVDRRG"
     gene            complement(7790..8977)
                     /locus_tag="VN968_11690"
     CDS             complement(7790..8977)
                     /locus_tag="VN968_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXL29936.1"
                     /translation="MLDATLRERASGLLTASGRSNVPPFMVMDVMAAAARIEAAGGHV
                     IHMEVGQPAASAPKTAISAAHTALDGGRIDYTSALGMPSLRDRIARHYRETHGCAADA
                     ERIVVTTGSSGAFILAFLALFEPGDRVAVTVPGYPPYRHILTALGCEPVLIETSSENR
                     HALTGEALLAAHRKNPLKGVLVGSPANPTGTMMSRDALTSLIAAAQGAGIRFISDEIY
                     HGLDYAFSAVTAAELSPDALVINSFSKYFCMTGWRVGWMVVPEPLVRPVERLQQNLSI
                     SVPTLSQIAAEAAFEGRLEMEEVKRGYLENRGILIAGLPQAGLSKFLPADGAFYLYAD
                     VSEFTSDSFKFASEMLEKAHVAATPGIDFDPVHGHRFIRFSYARSADEMREAVTRIAR
                     WLG"
     gene            9088..10494
                     /locus_tag="VN968_11695"
     CDS             9088..10494
                     /locus_tag="VN968_11695"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXL29937.1"
                     /translation="MLLRMALRKKMARLTAVTTAVALAIAPVPALAQQQKGPPVIRDT
                     ESEQLLREYTRPILRAAGLERQNIQIVIINESVFNAFVADGRRIFVNYGAMMQSETPN
                     QIIGVLAHETGHLAGGHLAKMREQMAQAQTQMIIAMLLGAGAMIAGSRGNNSNGGLAN
                     AGAAAVAGPQEMIRRTLISYVRQQEENADRAGVKFLTATGQSAKGMYETFKRFTNDSL
                     FAAHGADPYVQSHPMPAERVAALEEIARSSPYWEKKDDPALQLRHDMVRAKISAFMER
                     QDTVYRRYPMSNESLPARYAHAIATYLHGDLRSALAQIDALIQLQPANPYFYELRGQA
                     LLEGGKPAEAIAPLRRAVQLSNSAPLIEMLLGQALVGTDNKAYTEEAISILRAAVARE
                     TEAPLGYTQLAMAYGRKGDFAEADLASAQAAFLRGDNKTARELASRAKTRFAVGTPGW
                     VKADDIVAAKPMPGQSSN"
     gene            10556..11323
                     /locus_tag="VN968_11700"
     CDS             10556..11323
                     /locus_tag="VN968_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HXL29938.1"
                     /translation="MPSLRLLATALFALALCGMPPAASAQSFSDSQRGDIETIVRNYL
                     LAHPEVLEEAMGELSKRQAAADTEKHEAGIAKNAQTIFNSPRGVTVGNKDGDVTFVEF
                     FDYNCGYCKRAMADMLDLMKSDPKLKVVLKEFPVLGPGSVEAAQVAVAARMQDPAGKK
                     YLDFHQKLLGGRGQADKAHAMAAAKEAGFDMAKLEKDISSPEVRATLEENFKLAEDMG
                     MNGTPSYVIGKQIVVGAIGLDGLKEKIGLARCGKATC"
     gene            complement(11493..11696)
                     /locus_tag="VN968_11705"
     CDS             complement(11493..11696)
                     /locus_tag="VN968_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020047562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein, HigA family"
                     /protein_id="HXL29939.1"
                     /translation="MPITGLLGTSRQSLFDILRERQPAFRLGKLLGDRPDLWIDSQRA
                     HDLHAAEKQIAKALAKIPTISAA"
     gene            11838..12653
                     /locus_tag="VN968_11710"
     CDS             11838..12653
                     /locus_tag="VN968_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HXL29940.1"
                     /translation="MTSRFMMSVAAAALIAGTGFANAQGTGMGREAPSAQQSAPPSER
                     AAPSASPAARDAAEPTKPAPGAKATHSEQAPAAAKNQRAQDDMKAAPKGEKSAQDNDM
                     KGEKSKSMSSENAKGAKDLKAEGREDRSGMKAEGREDRTGTKAEGREGRDGNMKAEGR
                     EDRKGNMNAETKGAEGRSQTVGQAGAGAKLSTEQRTRISTVIKEQNIRPATNVNFAIS
                     VGTRVPREVSFHPLPVEIVNIYPDWRGYEMFLVNDQIVVVNPRTLEIVAVLDV"
     gene            12922..13398
                     /gene="aroQ"
                     /locus_tag="VN968_11715"
     CDS             12922..13398
                     /gene="aroQ"
                     /locus_tag="VN968_11715"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HXL29941.1"
                     /translation="MAKTAAETIYVLNGPNLNLLGTRELETYGHATLADVEKLCAETA
                     GQFGLKTDCRQSNREGELIDFIHEAHAAKAGGIIINAGGYSHTSIALHDALVAVKIPA
                     VEVHISNIHARESFRHHSFTAKAAFATLSGFGIDGYRLAVTGLAARIGAKTAKTTA"
     gene            13439..>13825
                     /locus_tag="VN968_11720"
     CDS             13439..>13825
                     /locus_tag="VN968_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HXL29942.1"
                     /translation="MARQPDDKPAAKFQSEDSALIRELALLLDETSLTEIEIERAGLR
                     VRVARNVSIAATMPANYQPTASASASASVAVADLAKHPGVVPSPMVGTAYWASEPGAK
                     PFIEVGTKVSVGQTLLIIEAMKTMNQI"
BASE COUNT         2678 a         4445 c         4222 g         2480 t
ORIGIN      
        1 cgcggaacat ggtgacgccg agggcgtaaa tgtcggtcgc ctcgttgccg ggttcgccgt
       61 caagcatttc gggcgccatg taggcagggg tacccggaat ctcctcgggc ggaaaatcct
      121 ccattccagg tatgcgcacc accccgagat cgatcagctt cagcgattcc tccccctcga
      181 ggatcacgtt gtccggcttg atgtcgcgat gcacgatgcc ggcgcggtgc agcgccgccg
      241 ccgcccgggc caacctgatc gcgatatgac gtccctcctc tagtcccacc ccaggcccac
      301 gcgtcagccg ggtctccagc aattcgccct gatagagcgg catgacggtg tagaggcagc
      361 tctgccggcc cggcggcagc tcgatgatgt ggccaagcca gggactggtc acgcgcgcgc
      421 ccacccacgc ctcgcggaca aaggccgcac ggtaggcggc gacgctggcc acctggggct
      481 tgggaaactt caacgctact tcgcccccct cgacctcgtc ctccgcaccg aacaggcggc
      541 tgtaccggcc atcggacagc agcactttaa gcacaaagcc atcgatcgtc tcgccgccga
      601 gcggcaccgg gatgatcggc agttgcgcga tgttggcgcc gatttcagcc gattccgccg
      661 tcggcaggcc cacgacgtcg agcaccagag cggtgcagtt gtcgctgctg tcggattgca
      721 gcgcagccgc caccaacgca cgcgcggtat cctcggaaga gacgcgctcg cgcatgatgt
      781 cggcgatggt ctctgccgcg aggtaggcgt gcacgccatc gctgcagagc agaaagcggt
      841 cgtgctgcgc caccggctgg ttggtgtagt ccagccgcac ctcggtctcg acacccaatg
      901 cgcgggtcag gatgttcgac cggccggcgc cgcccggccg tacatggtct gatgtcaggc
      961 aagtcaggcg gtcgcggctc agccggtagg cgcgggtgtc gccgacatgc agcacgtgcg
     1021 cgacgcgacc gcgcagcacc agcgccgtga aggtgcatcc catccccgtc agccttgtat
     1081 cccggcgacc ttcggaatag atccagccgt taagcgcatt cagaaccgtg gccgccgcac
     1141 gtcgcacttc catcgtctcg ggcagatcgc agaatccatc gaggaagccg cgcacggcga
     1201 tctcggccgc ctcgcgtccg ccttttgtgc tgccgatgcc atctgatatc gcggccgcca
     1261 cgtcccggcg cggttcgggc agttccgggc cgaaaacggc gccggcgaaa tcctcattgc
     1321 gcttgcgcgg cccggtctcc gaggcgaacc cgacgcttac cttgacgctg gaaatccgct
     1381 cagccatcgc cgccgctcca attttcgcgg ggcgcgcgcg cgcacggcgg gccgcctgga
     1441 cccgtgacgc ggcgatggcg ccgcctcgtt ttccgggatc acggcctcaa ttctgcagcc
     1501 ggcaccgatg atagaggaaa tagccgccgg tgctcgacgc cacgatcacc gtaagtttct
     1561 cggatttgcg gtcaagcgtg tagttcccgc catcggccgg ctcgtgccac gaaatctcag
     1621 cgtcgctgat gcgggccgga ttggcatcga ccgtagcctt gtcgtagtcg atcctgatct
     1681 gccaattcgt cccactggca acattggtgc aggtaaccgc ggtctcgccg cgcgccgcct
     1741 caccgctgaa agcgggaccg gccatgctga ccgcccacgc gcaaattccc gccgctagcc
     1801 gggacaaaat catggatcgc accagcacgc ctccctgcct atttattatt cacgatgcac
     1861 attctttaat cgcatcgacc tgtgttcgtt gcctgacttt tacggaaaat ccataagcaa
     1921 tcaatgtcca gttgggccgg caacgagtgg tatattcttt gcgttttgcc tgaacgctgg
     1981 tctgcgccac cggcgcacgg attggactta aaatcaaaat ggggaaacgt tcatggcact
     2041 cgtaccatca ccacaatggc tggatagcgg caataatgcc tggcagctcg cggcggcaac
     2101 atttgtcggc ctgcaaagca ttccagggct gacagttctc tacggcggca tcgtcaagaa
     2161 gaagtgggcg atcaattccg ccttcatggc gttctacgcg ttcgcttcgg tcctggtcgt
     2221 ctgggtcctg ttcgactaca atatggcgtt cgggccgcaa tggtttccat tcctgggtac
     2281 gccgggcctg gcgacatcgg ccacctttac gaccggtcag gcgatcattc cggcggcagc
     2341 atcgggcatg ccggcgctga cctttccgat ggcgacgctg attttcttcc agttcgtgtt
     2401 cgccgccatc accgtcatca tcctcgccgg ttccgtgctc ggccgcatga acttcacggc
     2461 gtggatgatc ttctgcccgg tgtggatgac cctggtctat acggtcgggg cgttcagcat
     2521 ctggggcggt ggctggctcg cagccatggg cgtcgccgac ttctcgggcg gctatgtcat
     2581 ccaccttgcc gccggcacgt ccggcttcgt cgccgcatgg ctggtcggtc cgcggctgca
     2641 ggccgatcgc gaccatttcc cgcccaacag cctgttgatg acgctggtcg gcgccggaat
     2701 cctgtggctg ggctggaacg gtttcaacgg cggcgatccc tatttcgcca acgccaatgc
     2761 cggcgcggcc gtgctcaaca ccaacacctg caccgcggtg gcccttctgg tctggacgct
     2821 gatggacaag atggcctatg gcaagccgtc ggtgctcggc gccgtcaacg gcatgatcgc
     2881 gggcctggtc gcgatcacgc ccggcgccgg ttacgtcgat ggcgtgggcg cgatcatgat
     2941 cggcatcgtc gccggcatca ttccctggct ggcgatgaac aagctgcaaa aggccagctt
     3001 catgatgaag gtggacgata cgctgagcgt cctctcgacc cacggcgtgg ccggcctcac
     3061 cggcggcttg ctgaccggca tcctggccaa tcccgcgatg ctgctgtaca tcggcaccga
     3121 caaggaagcg cccggcgtca acgtgaccgg cttgctctac ggcaacgcgc cccagctgct
     3181 gctgcaggcg gagggagccg ccttcatcat cgtctacaac gtgatcatga ccttcatcat
     3241 cctcaaggtg atctcgttca tcgtgccgct gcgcatggat gaggcaaccc tgaaggtggg
     3301 tgacgacgcc gttcacggcg agaccgccta tgcgattggt gtcgagggcg aatagccgac
     3361 cctctccacc tccagcgcag gacatggaat ccgcgggggc gcctccggtc cccgcggatt
     3421 tcttgcggcc gcgcacacgc cggccagcat tgaatgcgcc gggggactgc ccgccaccgc
     3481 caacgacatg gcgcgctgga tgaagtggca tctcgaccgc gttgcgaaag gtgttggcgc
     3541 gaggccaaca ttttccaagg cgccgatttc caagccgccg atgcaacctg agtgtcataa
     3601 ccagactttc gcacaaaact cctgcgatgc agcaatatga ctgccttcgg tgagatgcac
     3661 ctttcggtcg ccgcgagctg attatagatt ccccgaaatg ccccgttgag aatccggttc
     3721 ccacgggaca aggaatcaac cagggagggc cattcaatgg ctatggcaat cacacatgcg
     3781 acgttgtccg catccgatca cagcgcgcag ttgcgcaagg cggtcatcgc atcaaccatc
     3841 ggcaccgcca tcgagtggta cgacttcttc ctttatggca cggctgccgg gctcattttc
     3901 ggcaagctct actttcccaa cgaagatgca ttgacggcga cgctcgcggc ctttggcacc
     3961 tatttcatcg gcttcgtcgg gcgcccgatc ggtgccgcga tttttggtca ctatggtgac
     4021 cgcatcggcc gcaaggcgac actgatcgcc acgctgctgt gcatgggtat cgcgaccttt
     4081 ctcatcgcat tcgtgcctac ctatgcgtcg atcggcatct ggggcgcggt catcctcacg
     4141 gtgttgcgga tgctgcaggg tatcggcgtt ggtggcgaat ggggcggatc ggtgctgctg
     4201 gcgatggaat ggtcgcgcac ccacggtcaa cgcggtctgg tggcatcctg gccgcaattc
     4261 ggcgtgccgt gcggcctgtt tctggcaaac ctcgcagtcc tcgcattcag ccaattgtcg
     4321 ggtgaccagt tctccacctg gggctggcga attcctttcg cgctctcgat catcctggtt
     4381 ggcgtgggac tatggattcg gcttggcatc ctcgagacgc cggtgttcca gcagctcctg
     4441 gataccagga aaatcgagaa gacccccatc atcgaggtgt tcaagaagca tccgaaggag
     4501 atattcctgt cggcattgct gcggatgtcc gagcaagcgc cgttctatat cttcaccgcg
     4561 ttcatcttcg cctacgccgt cggtacgttg cacatgtcgc gcgatctgat cttgatcgcc
     4621 gtgctggttg cctcttgcgt ctcgttcgtc accatcccgc tgtcgggcca catctccgac
     4681 cgcatcgggc gcaggaagat gtatctgatc ggcgcggcga cggtgggcgt attcggtttc
     4741 ctgtattttg gcatggtgga caccgcgatt ccagccctgg tgttcatcgc aatcgtgctg
     4801 tcactcgtcc cgcatgacat gcaatatgga ccgcaggcag ccctgatcgc agaggccttt
     4861 acgccgcgat tgcgctacag cggtgcgtca ctgggctatc aactcgcatc ggtgatcgcc
     4921 ggcggtccgg cgcccctgat cgcgacagcc ctgttcgccg cttatgactc cggctacgcg
     4981 atttccgtct acatcgccgc ttgctcggtc gtaagcctgg tggcggcgtg gttcatgcct
     5041 gactacaccg gtcgggacat ttcgaaggag tatgacgact gacaccaagg tgtaacgtcg
     5101 gttgttcaag agggcccggg ctcgtccagg ccctctgatt ttgtcgggcg tgtccggcat
     5161 gggcttgatg ctgccgcaag agcatgatcc ggaaaagtgg gtaccggttt tgcatctcga
     5221 caaacgcgga acgcgtttgc gcggagatca tgctcaaaca aaaagataga gcgggattga
     5281 ggtcggtgcc aacctccggt gcaatgattt caccgggagg cagcgagggg ccgtcatgca
     5341 ctgtaacgat gccgaccaga tcctgaccgg cggcaaggat gaccgggacg aaaccgtcgc
     5401 gatcggcgat cgtcttcgtc atgtcgtcaa gaagaccctg ttcctgttga ctcaaggcgt
     5461 ggccatagac ccgctgttcg agatgtagcg aaagatattg gccacccgca gcaaaacgag
     5521 aatcgcgacg aaggcccctg cccagaggcc gggtgtaacg aggccattga tattgaagtc
     5581 catgcttgaa gctcccccag gtgcctcctg actacccctt gatgatttca cgcgcctgct
     5641 accccgttgt gacagccgtc tccacgtcgg agggatcgat cttcttgttg agatgggcat
     5701 gcagcctgtc gtgatcgagt tcgccttccc accacgacac gaacaccgtg gcgacgccat
     5761 tgccgatgat gttggtgagc gcgcgaacct cgctcatgaa cttgtccacc gaaaacacga
     5821 tcgccatgcc cggcaccagc gccggattaa ccaccgtaag cgtcgccgcc agcgtaatga
     5881 agccggcgcc ggtgacaccg ctggcacctt tcgacgtcag catggcgacg atcaggatgg
     5941 tcacttgctg gctgaacgac aggtcgacgc cgagcgcctg cgcaatgaac agcgtcgcca
     6001 gcgtcatata gatgttggtg ccgtccagat tgaacgaata gccggtcggc accaccagcc
     6061 cgaccaccgg cttggaacag cctagacgct cgagcttcgc catcaactgc ggcagcgcgc
     6121 tttccgacga actggtgccg agcacgatca gcaattcatc cttgatgtag gcgaggaact
     6181 tgaaaatatt gaagccgacg agccgcgcaa tcagcccgag cacgccgaat acgaacagcg
     6241 ccgcggtcat gtagaacacc gcgatcagtc cgaccagatt gccgagtgcg gcgggtccga
     6301 atttcccgat ggtgtaggcc atcgcgccaa aggcgccgag cggcgccgcc ttcatgacga
     6361 tggcgatcac gccgaacacc gcatgcgcgg tatcgtcgat gacttcgcgc agccggtggc
     6421 cgcgctcgcc gagcgccatc agcgcaaagc cgaacaggat cgcgaacagc agcacctgca
     6481 ggatgtcgcc gcgcgcgagc gcgccgacca cgctgtccgg aatgatgttg agaacgaaat
     6541 cgaccgactt ctgcgcttct gcctgcttga cgtaggtcgc aaccgccgcc gcgtccgggt
     6601 tcacggcgag cccgcggccg accgggatca gattgccgac cacgaggccg atgatcagcg
     6661 cgaacgatga gacgatctcg aaatagacca gcgccttgac cccgacgcgg ccgactttcc
     6721 gggcatcctg aatgtgcgaa atgcccgaca ccacggtgca gaagatgatc ggcgcgatca
     6781 ccatcttgat cagtttgatg aagccgtcgc cgagcgcctt gatccagtca ttggtcgcga
     6841 atgtgggcgc cagccagccg accaggacac ccagcaggat cgcgatcagc acctgcagat
     6901 aaagcacctt gtaccagggt cttgccgcgc cagcccgcgc atcggttgcg atcgttgccg
     6961 ccatgacctt ccctccccgt cagaattcgc ggatctgtct gtcgttgttc gcagacagcc
     7021 gtcccaccgg atacaaccaa gatcgaatgg ctgagcggag tcaatggcgt tgccttcgac
     7081 cgccggcggt ttagccgcgg cgatcgacca catgcctgat cgccggcacg agggcggctc
     7141 cgagtgcgtt cttgatcacg gacgcggcga agaacggcgc gatcccgacc agccatgcct
     7201 tctcgacgcc gagtttcatg ccaaacgcga gccacgcaaa tccggccgca agaatgacga
     7261 tgtggccaag tcccatcgcc acgaaaagcc gcgccacgga gcgatcccag ccacgctcgc
     7321 tcagccatcc cgtgatgaag gccgcgacga cgaaaccgaa gagatagccg gcggtcggcc
     7381 ccaccagcgg cgcgaggccg ccgacggcac tagcgaacac cggccatccg atcgccccca
     7441 tcgccagata ggccgtgacg gttgcgccgc cgagccgcgc gccataggcg gcgccgatca
     7501 tcagcacgac cagcgtttgc agcgtcatcg gcacgtaggg cagcggcaga ttgaccttgg
     7561 ccgagagcgt cagcagcgct gttcccaagg caatcaggat cacgatgcgc agcacgccgg
     7621 aaaccccttc gtcgcgatcg ggccacagca cctcggcgag tggaaagtgc ggcgtgacag
     7681 cgggctccgg gattgatctc gtggacaagg acatctcctg ttatgggcag gttgttcggc
     7741 gcgcagatga agaccgggcg aagaggggaa tcgaaagggc ttgcggcgct tatccgagcc
     7801 aacgcgcgat gcgcgtcacg gcttcgcgca tctcgtcagc ggagcgcgca taggaaaagc
     7861 ggatgaaccg atggccgtga accggatcga agtcgatccc gggggttgcc gccacatgcg
     7921 ccttttccag catctcgctg gcgaacttga aactgtcgga ggtgaactcc gatacatccg
     7981 cgtagagata aaacgccccg tcggcgggca ggaatttact gaggccggct tgcggcaggc
     8041 cggcgatcaa gatgccacgg ttctccaggt agccgcgttt gacttcttcc atctccagac
     8101 ggccctcgaa tgcagcttcg gctgctatct gcgacagcgt cgggaccgaa atcgagagat
     8161 tttgctgcag ccgttcgacc ggccgcacca gcggttccgg caccaccatc cagccgaccc
     8221 gccagccggt catgcagaaa tatttcgaga acgaattgat gacgagcgca tcgggagaaa
     8281 gctccgccgc cgtcaccgct gaaaacgcgt agtcgaggcc gtgataaatc tcgtcggaga
     8341 tgaaccggat acccgcgcct tgcgcggcag caatcaggct ggtgagcgcg tcgcgggaca
     8401 tcatcgtgcc ggtgggattg gccgggctgc ctaccagcac gcctttcagc ggattcttgc
     8461 gatgggcggc gagcaaggct tcacccgtca gcgcgtggcg gttttcgctc gatgtttcga
     8521 tcagcaccgg ctcgcagccg agcgcggtga ggatatggcg atatggcgga taaccgggaa
     8581 ccgtgaccgc aacgcgatcg cccggctcga acaaggcgag aaaggccagg atgaacgcgc
     8641 ccgaggaacc cgtggtgacc acaatccgct cggcatccgc ggcgcagccg tgcgtctcac
     8701 ggtaatgcct ggcgatgcgg tcgcgcagcg agggcatgcc cagcgccgag gtgtagtcga
     8761 tccggccgcc atcgagcgcg gtgtgagcgg ctgatatcgc ggtcttcggc gccgaagccg
     8821 ccggttgtcc cacttccatg tgaataacat ggccgccggc cgcttcaatg cgcgccgccg
     8881 ccgccatcac gtccatcacc atgaacggcg gaacattgct gcgccccgag gccgtcagca
     8941 ggccgctggc ccgttctctc aatgtcgcat ccagcatcga tatctgctat ttatcccggc
     9001 gtctcggtgc cccagactag ccgcattccg cagcttgtta gggcataacc cgtatccggc
     9061 ccgccgccaa cgattaaaga ttgctcaatg ttgctccgta tggcgctacg caaaaaaatg
     9121 gccagattga ccgctgtcac caccgcggtc gcgctcgcca ttgccccggt gcccgcgctg
     9181 gcgcagcagc aaaagggccc gccggtgatc cgcgataccg agtccgagca actgctgcgc
     9241 gaatacaccc ggccgatcct gcgggccgcg ggcctggaaa ggcagaatat ccagatcgtg
     9301 attatcaacg agagcgtttt caacgccttc gtcgccgatg gccgccgcat tttcgtgaac
     9361 tacggcgcga tgatgcagtc ggagaccccg aaccagatca tcggggtgct ggcgcatgaa
     9421 accggccatc tcgccggcgg ccatctcgcc aagatgcgcg agcagatggc ccaggcgcag
     9481 acccagatga tcatcgcgat gttgctcggc gccggcgcga tgatcgcggg ctcgagaggc
     9541 aacaacagca atggcggcct cgccaatgcc ggcgcggccg cggtcgcagg accgcaggaa
     9601 atgatccgcc gcaccctgat ctcctatgtg cgccagcagg aggaaaatgc cgaccgcgcc
     9661 ggggtgaagt ttctgaccgc gaccggccag tccgccaagg gcatgtacga aaccttcaag
     9721 cggtttacca atgacagcct gttcgccgcg catggcgccg atccttacgt ccagtcgcat
     9781 ccgatgccgg ccgagcgcgt cgcggcactg gaggaaatcg cgcggtccag tccctattgg
     9841 gaaaagaaag acgatccggc cctgcaactg cgccacgaca tggtgcgcgc caagatctcg
     9901 gccttcatgg agcggcagga caccgtctac cggcgctacc cgatgtcgaa cgagagcctg
     9961 cccgcgcgct atgcgcacgc gatcgcgacc tacctgcatg gcgatttgcg ctccgcgctc
    10021 gcccagatcg acgccctgat ccagctgcag cccgccaatc cgtatttcta tgagttgcgc
    10081 ggtcaggcgc tgctggaagg cggcaagccc gcggaggcca tcgcgccctt gcgaagggcg
    10141 gtgcagctct cgaatagcgc accgctcatc gagatgttac ttgggcaggc gctcgtggga
    10201 accgataata aggcctatac ggaagaagca atctcgattc tgcgcgccgc cgtggcacga
    10261 gagaccgaag caccgctcgg ctatacccag ctcgcaatgg cctatggccg aaagggagat
    10321 ttcgcagagg ccgacctggc atcggcgcag gccgcctttc ttcgcggcga caacaagacc
    10381 gcgcgcgaac tcgcctcccg cgcaaaaacc cgcttcgccg tcggcacccc cggatgggtc
    10441 aaggccgacg atatcgtcgc tgcaaagccg atgcccggcc aaagcagcaa ctagcgttaa
    10501 ttgagccagg tcaagaaggt tttcggacac cggctttcac caagaggatt tgcccatgcc
    10561 ttcgcttcgc ctgctcgcca ccgccctgtt cgcgctcgcg ctctgcggca tgccgcctgc
    10621 cgcgtcggcg cagagtttct ccgacagcca gcgcggggat atcgagacca tcgtgcggaa
    10681 ttatctgctg gcgcatcccg aagtgctgga agaggcgatg ggcgaactga gcaagcgcca
    10741 ggccgcggcc gacaccgaga aacatgaagc cggcattgcc aagaacgccc agacgatctt
    10801 caactcgccg cgcggcgtca ccgtcggcaa caaggatggc gacgtcactt ttgtcgagtt
    10861 cttcgattac aattgcggtt attgcaaacg tgcgatggcc gacatgctcg atctgatgaa
    10921 atccgatccg aaactgaaag tggtgctgaa ggaatttccg gtgctcgggc ccggctcggt
    10981 cgaggcggcg caggtcgccg tcgccgcgcg catgcaggac ccggccggca agaaatatct
    11041 cgacttccat cagaagctgc tcggcggccg cggccaggcc gacaaggccc acgcgatggc
    11101 ggcggccaaa gaagccggct tcgacatggc gaaactggaa aaggatattt cgagcccgga
    11161 ggtccgcgcg acgctggagg aaaacttcaa gctcgccgag gacatgggca tgaacggcac
    11221 gccgagctat gtgatcggca agcagatcgt ggtcggcgca atcggcctcg atggcctgaa
    11281 ggaaaagatc ggcctcgccc gctgcggcaa ggcgacgtgc tgaaggcttg gcacccgcct
    11341 gtttgtcagc gtgttcgttc aacaatccaa aagccggccg caatgcagcc ggcttttttg
    11401 ttcgcacgac cttcgacttg ggcctgcggc tgagagtcga ggcggcatcc ttcgcgttgc
    11461 gcacccctgc tcactccact cgcgagcgcg aactacgccg ccgatatcgt cggaattttc
    11521 gcgagcgctt ttgcgatctg cttttcggcg gcgtgcagat catgggcgcg ctgcgagtcg
    11581 atccaaaggt ccggccggtc gccaagcagc ttgccgagac gaaacgccgg ttgcctttcc
    11641 ctgaggatgt cgaacaggga ctgacgagaa gttccgagca ggccggtaat cggcactccc
    11701 taggattcac tcactcgcta ttcggttcat cgcgcattca caaaacaaaa ccggggggaa
    11761 ccgccgatat cacggaacat cgcgcatctc atctcgttgt cggcgcgggc aatgaaaaca
    11821 acgtgaggag atgttccatg accagtcgtt ttatgatgtc ggtggccgcg gcggcgctga
    11881 tcgccggcac cgggttcgcc aatgcgcagg ggaccggaat gggccgcgag gcgccatcgg
    11941 cgcagcagag cgcgccgccg tccgagcgcg ctgccccttc ggccagcccc gctgctcgcg
    12001 atgcagcaga accgaccaag cctgcgcccg gcgccaaggc cacgcattct gagcaagcgc
    12061 ctgctgccgc caagaaccag cgcgcgcagg acgatatgaa ggccgcgccg aagggcgaaa
    12121 aatcggccca ggacaacgac atgaagggcg aaaagtcgaa gagcatgagc tccgagaacg
    12181 ccaaaggggc taaggacctc aaggccgaag gccgtgagga ccggagcggc atgaaggccg
    12241 aaggccgtga ggaccgcacc ggcacgaagg ctgaaggccg cgaaggccgc gacggcaaca
    12301 tgaaggccga gggccgcgaa gaccgcaagg gcaacatgaa cgccgagaca aagggcgcgg
    12361 aaggcaggtc gcagaccgtc ggacaggccg gcgccggcgc aaaactctcg actgaacagc
    12421 gcaccaggat cagcaccgtg atcaaggagc agaacatccg gccggcgacc aacgtcaatt
    12481 tcgcgatctc ggtcggtacc cgcgttccgc gcgaggtctc gttccacccg cttccggtgg
    12541 aaatcgtcaa catctacccg gactggcgcg gctacgagat gttcctcgtc aacgatcaga
    12601 tcgtcgtggt gaatccgcgc acgctcgaga tcgttgccgt gctcgacgtc tagcactgag
    12661 cgcatggcga gtttgggggc gggcagcgat gcccgcccct ttttgcgtcg ggcgcggcga
    12721 gcaaggaacc acgcctgccc cggcaggtcg gcgccaccgc cattcgcaac attggttaac
    12781 gtgtgatttc cacagcttat gcgcaccttt ttgcgagccg gatgagccat ctgaaggaag
    12841 cgcaagtcgg cctaaaggct tccccttggt cctgttgtta cctataaccg cgccacctac
    12901 cgccacattg ccgggatatc gatggccaag acagctgccg agacgattta cgtgctcaac
    12961 ggcccgaacc tcaatctgct ggggacgcgc gagctcgaga cctatgggca tgcgacgctt
    13021 gccgatgtcg agaaactttg cgccgagacc gcggggcaat tcggcttgaa gaccgattgc
    13081 aggcagtcca atcgcgaagg cgagctgatc gatttcatcc acgaggcgca tgctgcgaag
    13141 gcaggcggga tcatcatcaa tgccgggggc tattcccaca cctcgatcgc actgcatgac
    13201 gctttggtgg cggtgaaaat tccggccgtc gaagtgcaca tcagcaacat tcacgcccgc
    13261 gagagctttc gtcatcattc cttcactgcc aaagccgcct tcgcgacgct ttcaggtttc
    13321 ggcatcgatg gctaccggct cgcggtcacc ggccttgccg ccaggatcgg cgccaagaca
    13381 gccaagacaa cagcctgacg cgttagccgt cacccacaga aactttcgga tccagatcat
    13441 ggcgcgccag cctgacgaca aaccagccgc aaaattccag agcgaggata gtgcactcat
    13501 ccgcgaactc gcacttctgc ttgacgagac cagtctcacc gagatcgaga tcgagcgcgc
    13561 gggcctgcgg gtgcgggtcg cccgcaacgt cagcatcgcc gcaacgatgc cggcgaatta
    13621 tcaacccacg gcttccgcgt cggcctcagc gtcggtcgcc gtcgccgatc tcgccaagca
    13681 cccgggcgtg gtgccctcgc cgatggtcgg caccgcctac tgggcttccg agcccggcgc
    13741 caagccgttc atcgaagtcg gcaccaaggt gtcggtcgga cagacgctgc tgatcatcga
    13801 ggcgatgaag acgatgaacc agatt
//
LOCUS       DATNOI010000298        10247 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_920957,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000298 DATNOI010000000
VERSION     DATNOI010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10247
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920957"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..693)
                     /locus_tag="VN968_11725"
     CDS             complement(103..693)
                     /locus_tag="VN968_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HXL29943.1"
                     /translation="MRKTQKAAAKRTGAAVKPKQRPATIERPGSWHLARTETERRLSE
                     FEFRLERLAQAYYRWKASCLAAVCDVPLTGDDVAVLNVVRMGDEPKRLSEIGQLLNRV
                     DVPNLQYATRKLVKAGLIETEGSSSRKETRYRATETGYSVTEAYATLRAATLPPMLEA
                     LEGWAEKSEAAGSHLELVSSLYTQAAQVAITRRHQP"
     gene            895..2133
                     /locus_tag="VN968_11730"
     CDS             895..2133
                     /locus_tag="VN968_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXL29944.1"
                     /translation="MAGQWIDIAAKDGGSFKGYLAVPASGSGPGILLLQEIFGVNKSM
                     REVADHYAEEGYVVLAPDLFWRMEPGVELGYTEADFGKAMGYYQRFDANQSIRDAADA
                     LKVLRARPECKSKVGALGFCLGGKLAYLAAARTDVDCAVSYYGVGIEADLAEAHNIKG
                     PMVFHFAELDRFAPTEAREQVKAAFKGRSDVEFYLYPGCDHAFAAPERSSFDKPATLM
                     AHTRSIALLRRVLGPNYDLSALWDRHTELEFATRSAEQTMTTMVAQPYVNHIPTMTGG
                     VGYRDLLRFYKNHFIPKTPQDTKLVPISRTIGSDRIVDEMLFCFTHDIEIDWMLPGVP
                     PTGKYVEIPLVAIVRFRGDKLYNEHIYWDQASVLVQIGLLDPKKLPVAGVETAKKLVD
                     EALPSNTLMARWSESAGNQN"
     gene            2193..3737
                     /locus_tag="VN968_11735"
     CDS             2193..3737
                     /locus_tag="VN968_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL29945.1"
                     /translation="MVSSLSRRSILQLAGGAAALPLASGFIGAGVAQAAAGTLTIAYN
                     VNLPSFDPTIGVSAVNPTIQSIYQSVFDPYIGQAPDLSFKPGLLTKWGWSEDRSKITL
                     ELRKGATWHDGSPVTPEDVVWSLQRAGDPKGGNPIQFVWSKVGNYKIDGNTITGDVLE
                     FEPTLFKWMAFLTGYILPKAYFEKVGAEGFEKAPIGSGPYKVDAFERNAFLRLKAHAG
                     YWGGKPAFDTVVFKFITDPTSRVAEMESGGSDLTFEIPYEEFDRLKGKPGLVGQTKPV
                     SDIAMIFLTDIDPMLDRNVRLAANHAIDKKAIVERLLKGYGVPISTLEAPGYAAFDPS
                     IKVAYDPALAKSLLAKSGYSPEKPVKFTIQTTRGFKPKDYEMIQAIVGMWRKVGIDAT
                     IEVYEIAKHFELRARHALAPAAFYNWGNAIGDPSTSTGFAMFGPSPHCAWKGKDLVER
                     IGPLWGEKDEAKRIAGYKAVDKYIAEEGEVIPLFQYVQPIIHKKGLKVVPQANGMILP
                     HLITPA"
     gene            3767..4774
                     /locus_tag="VN968_11740"
     CDS             3767..4774
                     /locus_tag="VN968_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL29946.1"
                     /translation="MRWGEIIKRLVLVPPTLFGVAVIVFVLLRVVPGDPIAMMIPPGA
                     SEADITRLRALYGLDQPIIQQFTTWLGQVARGDFGRSISVHQGVLDLLLARLPATIEL
                     ALLATMMAVVLGVLVALTGILLRGRRAEWAVDGGVGGLLAIPDFLWALILLLLFGVLI
                     PWLPISGRIDPQIDINLRSNFYLLESLLTGRFDIASALLHHMILPALALALPFAALVA
                     RILKASLAEAEDQDYAQIARARGYSRPAILLHEVFPNALIPTVALGGVQVTLLLGGTV
                     LVERIFSYEGIGNMAIDAVINRDFPLIQGLVLTFAVLFITLNLAVDFVVTLLDPRLRH
                     G"
     gene            4767..5654
                     /locus_tag="VN968_11745"
     CDS             4767..5654
                     /locus_tag="VN968_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL29947.1"
                     /translation="MADLALGSPVARQSRLLAGLKRGGARLWIGAALVAVLVLAALLA
                     PAIAPHDPLEQDLMSAQLPPAWMVGGDPAYLLGTDSLGRCVLSRLIYSARTAVAVALI
                     AATLAALIGVALGLFAGSFGGWVDQLISRLIDVWMAFPPVLLSIVLAAVIGAGLTSVI
                     AAIVVVDWTRFARVVRAETMVQLTRDYAAAARVIGMSRAKLLRLEILPNLVPLLVTLL
                     AVEMGIAILVEVILSFVGISVSGDTPTWGSMIAEGRQIVYQAPWIMALPIVCIITSVI
                     GLNLLGDGLRLALDPVQRS"
     gene            5651..6526
                     /locus_tag="VN968_11750"
     CDS             5651..6526
                     /locus_tag="VN968_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL29948.1"
                     /translation="MTATLAVEGLKVAIGAANVLRGVSLALQPGEVRGLVGESGAGKS
                     MLGRAVLGLLPANATIISGHIAFEGRDLLDMRETERRGLLGRRIALIPQDPMTSLNPV
                     KRVGAQIGSLLRHHLGLSKPAALTRATELLTEVAIREPDRVLSLYPHEISGGMRQRVL
                     IAMAFACDPGLVIADEPTTALDVTVQRQVLQLVERLRRRHGSAVLFVTHDLGVVAKIC
                     RSMTVLHTGRVLEEGQTADVLARPRHLYTRALLAATPRADRPAEALRPVPPALIEQLW
                     SEAHRLDQPAIAGPA"
     gene            6610..7380
                     /locus_tag="VN968_11755"
     CDS             6610..7380
                     /locus_tag="VN968_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL29949.1"
                     /translation="MIKRVDLAVPRGSVLGLVGESGSGKTTLGRLLVRLLQPTSGGIQ
                     FDDVEIGQLDEAELRPLRTRIQMIFQDPQSSLNPRLRLGTTLTRPLGVHGRLRGQRDQ
                     RERAAALLDLVGLPPDFADRYPHELSGGQRQRAGIARALALEPDFIVADEIVSGLDVS
                     TQAHILLLLKELRARLGLTMVFISHDLSVVRVLCDDVAILHHGEVVEYGAVARVFGAP
                     QHAYTQQLLAAIPLPDIEPGWLDDAPPPVTSQQQGSAA"
     gene            7458..8060
                     /locus_tag="VN968_11760"
     CDS             7458..8060
                     /locus_tag="VN968_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL29950.1"
                     /translation="MTKSRTLLAFAALALSAAASSASADSIPGLRGHDHTGITVPDVK
                     AATAFFTDVLGCAHAMSFGPFSDDKGTFMQDAVNVNPRAVIDEISMVRCGYGSNIELF
                     QYQSPDQAKTLPKNSDIGGHHIALYVDDIAKAAEYLRSKNVKTMQGPIPVNEGPAAGQ
                     SILYFLTPWGLQMELISYPKGMAYEKSAKTVLWSTTEPMK"
     gene            complement(8172..8381)
                     /locus_tag="VN968_11765"
     CDS             complement(8172..8381)
                     /locus_tag="VN968_11765"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29951.1"
                     /translation="MKSGAAIDGAELVRYCEGRLSYFAIPRFIDFVADLPRTESGKVQ
                     KFKLREQGRSAQTWDREAAGIKLKR"
     gene            complement(8514..10007)
                     /locus_tag="VN968_11770"
     CDS             complement(8514..10007)
                     /locus_tag="VN968_11770"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL29952.1"
                     /translation="MVKQKHVCIIGAGVSGLAAGKAFSERGHRVTIIERGGNLGGVWD
                     PARSYPDVQTQSPKELYRYTDKAMPESYPEWPKGPQVHAYLSGYARDHKLDSSMRFNT
                     TVVSMDRRADGKAGWTLALRGRDGASGREDFDFVAICTGQFNEPQTLSLPGEEPFKAQ
                     GGEILHSSQYNDPAIAKGRKVVVLGGSKSATDIAVNAVNSGAAEVTIVYREPVWRIPY
                     FIGGLVNFKRILYIRAQEEMFQSWGIGAMSRFKHALAKPFVWANWRGLESLLKLQLKL
                     KQCDMVPKEPIENGVNCSVPIATPGFHTMVADGRIKAVRGTFAHYDGKTIVMSGGERV
                     AADVAVLAIGYKLGVPFLPESYRAKLVEPDGQYRLYRLIANPDLPDLGFVGFNSSFCT
                     VLCADMAADWLVRYADGQLARQPSAAEMNENIAMMLRFKRDERPAAGVYGGLCVAPYH
                     FKHFDELLADIGAATRRRNPLAEKFTPPDADAYARFLASAPSYMAAA"
BASE COUNT         1780 a         3332 c         3204 g         1931 t
ORIGIN      
        1 ttcaaaagat accgttcagg acgcgtcggt cgtcaacacg ggaatgccac cagcatcatg
       61 agtattccgc tgcgccgtga tcgcggtccg cccagcgccc ctttagggct gatgccgccg
      121 cgtgatcgcg acctgtgcgg cctgcgtata aaggcttgag accagctcga gatggctgcc
      181 cgccgcctcg gatttttccg cccagccttc gagtgcctcc agcatcggcg gcagggtcgc
      241 ggcgcgtaag gtcgcatagg cttcggtgac ggagtagccg gtctcggtgg cgcggtagcg
      301 cgtctccttg cgcgacgacg agccctcggt ctcgatcagg ccggccttca ccagctttcg
      361 cgtggcgtat tgcagattgg ggacgtcgac gcggttgagc aattggccga tctcggagag
      421 ccgctttggc tcgtcgccca tcctgaccac gttgagcacg gcgacgtcgt cgccggtcag
      481 cggcacgtcg cagacagcgg caaggcacga ggccttccag cggtaatagg cctgcgccag
      541 ccgctccagc cggaattcga attcggagag ccgccgctcg gtctcggtgc gcgcgagatg
      601 ccaggatccc ggccgctcga tggtggccgg ccgttgcttt ggcttgaccg ccgcgccggt
      661 tcgcttcgct gcggccttct gcgtcttcct caacatcgtc tcctgattcc cacccttcgc
      721 tcacgtcctg ggcaggctgt gctcaaatct atgctattta caagttcgat tcttacatta
      781 aaagtacaat taagaaagca tgatgaaaag gcaatagccc aggtcgtgcc agaacgcgtc
      841 aaacggacgc gcagaacggg aaggctcaag ggctttagcg acgagagggg atcgatggcg
      901 ggacaatgga tcgacatcgc tgccaaggac ggcggcagct tcaagggtta tctcgcggtc
      961 cccgcgtcag gctccggtcc cggcatcctg ctgttgcagg agatctttgg cgtcaacaaa
     1021 tcgatgcgcg aggtcgccga ccactacgct gaggaaggct atgtggtgct ggcgccggat
     1081 ctgttctggc gcatggagcc cggcgtcgag ctcggttaca ccgaggccga tttcggcaaa
     1141 gcgatgggct actaccagcg cttcgatgcc aaccagtcga tcagggatgc ggccgatgcg
     1201 ctcaaggtgc tgcgtgcgcg gcccgaatgc aaaagcaagg tcggcgcgct cggcttctgc
     1261 ctcggcggca agctcgccta tctcgccgct gcgcggaccg acgtcgattg cgccgtcagc
     1321 tattatggcg tcggcatcga ggccgatctc gctgaagccc acaatatcaa gggaccgatg
     1381 gtcttccatt tcgcggagct cgatcgcttc gcgccgacag aggcgcgcga gcaagtcaag
     1441 gcggcgttca aggggcgttc cgacgtcgag ttctatctct atcccggctg cgaccatgcc
     1501 tttgcggcac cggagcgatc gagctttgac aagcccgcga ccttgatggc gcatacgcgc
     1561 tcgatcgcgc tgttgcgccg ggttctcggc ccgaactacg acctctccgc attgtgggac
     1621 aggcacaccg agctcgaatt cgcgacgcgt tcggccgaac aaaccatgac gaccatggtc
     1681 gcccagccct atgtcaacca catcccgacc atgaccggcg gcgtcggcta tcgcgacctc
     1741 ctgcgcttct acaagaacca tttcatcccg aagacgccgc aggacaccaa gctggtgccg
     1801 atctcgcgca ccatcggctc cgaccgcatc gtcgacgaga tgctgttctg cttcacccac
     1861 gacatcgaaa tcgactggat gctgcccggc gtaccgccga ccggcaaata tgtcgagatt
     1921 cccctggtcg cgatcgttcg cttccgcggc gacaagctct acaacgagca catctattgg
     1981 gatcaggcct cggtgctggt gcagatcgga ctgctcgatc ccaagaagct gccggtcgcc
     2041 ggcgtcgaga ccgcgaagaa gctggtcgac gaagcgctgc cgtcgaacac gctgatggcg
     2101 cgctggtccg agagcgccgg taaccagaac tgatcgacgg cgcccgcttg ggcgccgttt
     2161 tcgcgcgtgg ctcatatagt ggggtaatca acatggtgtc ttcgctgtct cgtcgttcga
     2221 tcctgcaact cgccggcggc gcagccgcgc tgccgttggc gtccggtttc atcggtgctg
     2281 gagtggcgca agccgctgcg ggcacgctga ccatcgctta caacgtcaac ctgccgtcgt
     2341 tcgatccgac catcggagtt tcggcggtca accccacgat ccagtcgatc tatcagtccg
     2401 tgttcgatcc ctacatcggc caagcgccgg acctctcgtt caagccgggc ctcctgacca
     2461 aatggggctg gagcgaggat cgcagcaaga tcacgctcga gctgcgcaag ggggcgacct
     2521 ggcacgacgg ctcgccggtg acgcccgagg acgtggtttg gtcgctgcaa cgggccggcg
     2581 atccaaaggg cggcaatccg atccagttcg tctggtccaa ggtcggcaac tacaagatcg
     2641 acggcaacac cattaccggc gacgtgctgg agttcgagcc gacgctgttc aagtggatgg
     2701 cgttcctcac cggctacatc cttccgaaag cctatttcga gaaggtcggc gcggaaggct
     2761 ttgagaaggc gccgatcgga tctggcccgt acaaggtcga cgccttcgag cgcaacgcgt
     2821 tcctgcgcct gaaggcgcat gccggctatt ggggcggcaa gcccgccttc gacacggtgg
     2881 tgttcaagtt catcaccgat cccaccagcc gcgtcgccga aatggaatcc ggcggatcgg
     2941 acctcacctt cgaaatcccc tacgaggaat tcgaccgtct gaagggcaag ccggggctgg
     3001 tcggccagac aaaaccggtg tccgacatcg ccatgatctt cctcaccgat atcgacccga
     3061 tgctcgatcg caatgtacgc ctcgcggcga accacgccat agacaagaaa gcgatcgtcg
     3121 aacgcctttt gaagggttac ggcgttccta tatcgacgct ggaagcaccg ggctatgccg
     3181 ccttcgatcc ctcgatcaag gtcgcctacg atccggcgct tgcgaagagc ctgctcgcga
     3241 agagcggcta ctcgccggag aagcctgtca agttcacgat ccagaccacg cgcggcttca
     3301 agccgaagga ctacgagatg atccaggcga tcgtcggcat gtggcgcaag gtcggcatcg
     3361 acgccaccat cgaggtctac gagatcgcaa aacatttcga attgcgcgcg cgccacgcgc
     3421 tggcgccggc cgccttctac aattggggca atgcgatcgg cgatccctcg acctcgaccg
     3481 gctttgcgat gttcggcccg tcgccgcatt gcgcctggaa gggcaaggac ctcgtcgagc
     3541 gcatcggacc gctgtggggc gagaaggacg aggccaagcg catcgccggc tacaaggcgg
     3601 tcgacaaata tatcgccgag gagggcgagg tgatcccgct tttccagtat gtgcagccaa
     3661 tcatccacaa gaaaggactg aaggtcgtgc cgcaggcgaa cggcatgatc cttccgcacc
     3721 tcatcacgcc cgcctgagcg gcgccaagca gcggggtgac ctactgatgc gatggggcga
     3781 gatcatcaag cgactcgtcc ttgtgccgcc cactctcttt ggcgtggcgg tgatcgtctt
     3841 cgtgctgctg cgcgtggtgc cgggcgatcc cattgccatg atgattccgc ccggcgcatc
     3901 ggaagcggat atcacccgcc tgcgcgcgct ctacggcctc gaccagccga tcatccagca
     3961 gttcacgacc tggctcggcc aggtcgcgcg cggcgatttc ggccgttcga tcagcgtgca
     4021 tcagggtgtc ctcgacctgc tgttggcgcg cctgccggcg acgatcgagc tcgcactgct
     4081 cgcgacaatg atggccgtcg tgctcggcgt cctggtcgcg ctcaccggca tcctcttgcg
     4141 cggccggcgg gcggaatggg cggtcgatgg tggcgtcggc ggcctgctcg ccattcccga
     4201 ttttctctgg gcgctgatcc tcttgctgtt gttcggtgtg ctgatccctt ggctgccgat
     4261 ctccggccgc atcgatccgc agatcgatat caacctgcgc agcaatttct acctgctcga
     4321 aagcctcttg accggccgct tcgatatcgc gtccgcgctt ctgcaccaca tgatcctgcc
     4381 ggcgctggcg ctggcgctgc cgtttgcggc gctggtggcg cggatactga aagcgtcgct
     4441 ggcggaggcc gaagaccagg actacgccca gatcgcgcgc gcccgcggct attcccggcc
     4501 ggccatcctg ctgcacgagg tgtttccgaa cgcgctgatc ccgaccgtcg cgctcggcgg
     4561 cgtccaggtg acgctactgc tcggcggcac cgtgctggtc gagcgcatct tctcctatga
     4621 gggcatcggc aacatggcga tcgatgccgt gatcaaccgc gactttccgc tgatccaggg
     4681 cctggtgctg accttcgcgg tgctgttcat cacgctcaac ctcgccgtcg atttcgtggt
     4741 gacgctgctc gatccgaggc tgcgccatgg ctgaccttgc ccttggttct cccgtcgcac
     4801 ggcaatcgcg cttgctcgcc ggcttgaagc ggggcggcgc gcgattgtgg atcggcgcgg
     4861 cgctggtcgc cgtgctggtg ctcgccgcgc tgctcgcgcc ggcgatcgca ccgcatgatc
     4921 cgctcgagca ggatctgatg tcggcacaac tgccgccggc ctggatggtc ggcggcgatc
     4981 ccgcctacct gctcgggacc gacagtctcg gccgctgcgt gctgtcgcgg ctgatctatt
     5041 cggcgcgcac cgcggtcgcg gtggcgctga tcgcggcgac gctggcggct ttgatcggcg
     5101 tcgcgctcgg cctgttcgcc ggcagcttcg gcggctgggt cgatcagctc atctcgcgac
     5161 tgatcgacgt ctggatggcg tttccgccgg tgttgctctc gatcgtgctg gcggcggtga
     5221 tcggtgccgg cctgacctcg gtgatcgcgg cgatcgtcgt ggtcgattgg acccgctttg
     5281 cccgcgtggt gcgcgccgaa accatggtgc agttgacgcg cgactacgcc gcggcggcgc
     5341 gtgtcatcgg aatgagccgg gcaaaactgt tgcggctgga gatcctgccc aatctcgttc
     5401 cgctgctggt cacgctgctc gcggtcgaga tggggatcgc gatcctcgta gaggtcatcc
     5461 tgtcgtttgt cggcatctcc gtgtcgggcg acaccccgac ctggggcagc atgatcgcgg
     5521 agggtcgcca gatcgtctac caggcgccgt ggatcatggc gctgccgatc gtctgcatca
     5581 tcacgagtgt gatcggcctc aacctgctcg gcgatggctt gcgtctcgcg ctcgatccgg
     5641 tgcagcgatc atgaccgcca cgctcgccgt cgagggtctg aaggttgcga tcggcgccgc
     5701 caacgtgctg cgcggcgtga gcctcgcgct gcaaccgggc gaagtccgcg ggctggtcgg
     5761 cgaatccggc gccggaaaat ccatgctggg ccgagccgtg ctcgggctgt tgccggcgaa
     5821 cgcgaccatc atttcgggcc acatcgcctt cgaggggcgc gatctcctgg acatgcggga
     5881 gaccgagcgt cgcggcctgc tcggccgccg cattgcactg atcccgcagg atccgatgac
     5941 gtcgctcaat ccggtcaagc gcgtcggtgc gcagatcgga agtctgttgc ggcatcatct
     6001 cggcctgtcg aagccggcgg cgctcacgcg cgcgacagaa ctcctgaccg aagtcgcgat
     6061 ccgcgaaccc gatcgggtac tgtccctcta ccctcacgaa atctccggcg gcatgcggca
     6121 gcgcgtgctg atcgcgatgg cctttgcctg cgatcccggt ctggtgatcg cggacgaacc
     6181 gaccaccgcg ctcgacgtca ccgtgcagcg ccaggtgctc cagcttgtcg aacgtctccg
     6241 acggcgccat ggcagcgccg tgctgttcgt gacgcatgat ctgggcgtgg tcgcaaagat
     6301 ctgccgcagc atgacggtcc tgcacaccgg ccgcgtgctc gaagagggcc agaccgcgga
     6361 cgtgctggcg cgaccgcgtc acctctatac gcgcgcgctc ctcgcggcga cgccgcgcgc
     6421 ggatcggccg gccgaggcgc tgcgtccagt gccgccggcc ttgatcgaac aattgtggtc
     6481 ggaagcgcac cggcttgacc agccggcaat agcggggccg gcatgagcga catcctgctt
     6541 cgcacctctg acctaagctt tggctatccg gagcgtaacg ggctatttcg gaaaccggcc
     6601 ctgcgcgagg tgatcaagcg cgtcgacctc gccgtgccgc gcggctcggt gcttggcctc
     6661 gtcggcgaat ccggctcggg caagaccacg ctcggccggc ttttggtgcg gctgctccag
     6721 ccgacctccg gcggtatcca gttcgacgat gtcgagatcg ggcaactcga cgaggccgag
     6781 ctgcggccgc tgcgtacgcg tattcagatg atctttcagg atccgcagtc ttcgctcaat
     6841 ccgcggctgc ggctcggcac caccttgacc cggccgctcg gcgtgcatgg gcggctaagg
     6901 ggacaacggg atcagcgcga gcgcgcggcg gcgctcctcg atctcgtcgg cctgccgccc
     6961 gacttcgccg atcgctatcc ccacgagctt tccggcggcc agcgccagcg cgcaggcatc
     7021 gcgcgtgcgc tggcgctcga accggacttc atcgtcgccg acgagatcgt gtcgggcctg
     7081 gacgtgtcga cgcaggcgca catcctgctg ctactgaagg agctgcgcgc gaggctcggc
     7141 ctcaccatgg tcttcatcag ccacgacctc tcggtcgtgc gcgtgctctg cgacgacgtc
     7201 gcgatcctgc accatggcga ggtcgtcgaa tatggagccg tcgcaagagt cttcggtgct
     7261 ccgcagcacg cttacaccca gcaattgctc gcagcgatcc cgttgcccga tatcgagccg
     7321 ggttggctcg acgatgcccc accgcctgtc acctcccagc agcaaggatc agccgcatga
     7381 tccgagaggt ggcgagcacc aactggcctc gctctcgcac cagagtttca actgactgtc
     7441 aaccaaagga agcgatcatg accaagtcta gaacactgct tgccttcgcc gcactcgcgc
     7501 tttccgccgc cgcatcgtcg gcctccgctg attccattcc aggcttgcgc ggccacgacc
     7561 ataccggcat caccgtaccc gacgtgaaag ccgcaaccgc gttctttacc gacgtgctcg
     7621 gctgcgcgca tgcgatgtcg ttcgggccgt tttcggacga caagggcacg ttcatgcagg
     7681 acgccgtcaa cgtgaacccg cgcgccgtca tcgacgagat cagcatggtc cgctgcggct
     7741 atggatccaa catcgagctg ttccagtacc agtcgcccga ccaggccaag acgctgccga
     7801 agaacagcga catcggcggc caccacatcg cgctctatgt cgacgacatt gcgaaggcgg
     7861 cagaatatct gcggtcgaag aacgtcaaaa ccatgcaggg accgatcccg gtcaatgaag
     7921 ggccggcggc cggccagtcc atcctctact tcctgacacc gtggggcctg cagatggagc
     7981 tgatcagcta tcccaagggc atggcctatg agaagagcgc caagaccgtg ctgtggtcga
     8041 cgacggagcc gatgaagtag cgcgtaccga gtatctttcc gttaaaatag aacagccatc
     8101 ccttctgagg gatggactgc cggatcaagc acgacaatgg cgcggcgcca tgtgcgggca
     8161 tcatgcttcg ctcatcgctt gagcttaata cctgcagctt cgcggtccca agtctgtgcc
     8221 gaacggcctt gctcgcgcag tttgaatttt tgcaccttgc cgctttcagt acgcggcagg
     8281 tcggcgacga aatcaataaa gcggggaatg gcgaaataag acagccggcc ctcgcaatag
     8341 cggacgagtt cggcaccatc gatcgcggcg ccggatttca ccgctacagc gttctggcga
     8401 gccttcaaga gttggaccgg agaaagccct cgaaccgcgc gaaacagcaa cagtcgcgtt
     8461 ggccgttcac cggatcgtct cacattgagc agcggctgga tgcgccgttg gtttcacgcc
     8521 gccgccatgt agctcggggc cgatgcgagg aagcgcgcat aggcgtcggc gtccggcggc
     8581 gtgaatttct ccgccagcgg attgcggcgg cgcgtcgccg ctccaatgtc cgccagcagt
     8641 tcgtcgaagt gcttgaaatg gtacggcgcg acacagagcc cgccatagac gcccgccgcc
     8701 ggccgctcgt cgcgcttgaa gcgcaacatc atggcgatgt tctcgttcat ctcggccgca
     8761 ctcggctgtc gcgccagttg gccgtcggca tagcgcacca gccagtcggc cgccatgtcg
     8821 gcgcacaaca ccgtacagaa gctcgaattg aacccgacaa agcccaagtc cggcaagtcc
     8881 ggattggcga tcaggcgata gagccggtat tggccgtcgg gctcgaccag cttggcgcga
     8941 taggattcgg gaagaaaggg cacgccgagc ttgtagccga tcgccagcac cgccacgtcg
     9001 gcggcgacgc gctcgccgcc gctcatcacg atggtcttgc cgtcgtaatg ggcgaacgta
     9061 ccgcgcaccg ccttgatgcg accatcggcc accatcgtat ggaaaccggg cgttgcaatc
     9121 gggaccgagc agttgacgcc gttttcgatc ggctccttcg gaaccatgtc gcactgctta
     9181 agcttgagtt gcagcttcaa caggctctcc agtccgcgcc agttcgccca gacaaagggt
     9241 ttcgccagcg cgtgcttgaa ccgcgacatt gcgccaatac cccagctctg aaacatctct
     9301 tcctgtgcgc ggatgtagag gatgcgcttg aagttgacca ggccgccgat gaagtacgga
     9361 atgcgccata ccggttcgcg atagacgatg gtgacttcgg cagcgccaga attcaccgcg
     9421 ttcactgcga tgtcggttgc ggatttcgag ccgccgagca cgaccacctt gcgtcctttg
     9481 gcgattgcgg gatcgttgta ttgcgacgaa tgcaggattt ctccgccttg cgccttgaag
     9541 ggctcttcgc cggggaggct gagagtttgc ggctcgttga attggccggt gcatatggcg
     9601 acgaaatcaa agtcctcacg gccgctggcc ccgtccctac cacgcagcgc gagtgtccat
     9661 cccgccttgc cgtcggcacg gcggtccatc gacacgacgg tggtattgaa gcgcatcgag
     9721 ctgtcgagct tgtggtcgcg ggcatagccg gagagatagg catggacctg cgggcccttc
     9781 ggccactccg gataggattc cggcatcgcc ttgtcggtat agcgatagag ctccttcggg
     9841 ctctgggtct gaacatccgg gtaggaacgt gcaggatccc agacgccgcc caaattcccg
     9901 ccgcgctcga tgatggtcac gcggtgtccg cgctcggaaa acgccttacc cgcggcgagt
     9961 cccgatacgc cggcgccgat gatgcagacg tgcttctgct tgaccatgaa catgctcccg
    10021 taaattgaag gattccacct gctgaattga acgcgtgcgc ccgtcattgc cggggcgccc
    10081 cgggcatccc gcaacgaaat gttcgaccta gtacctggaa tcacaaatcg gtattgcagc
    10141 ccatccttcg agacggccac ttcgtgacct cctcaggatg aggtctaact cgttgaaaca
    10201 caacaacctc atgctgagga gcgagcgacg cgagcgtctc gaagcat
//
LOCUS       DATNOI010000299         3077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2540501,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000299 DATNOI010000000
VERSION     DATNOI010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2540501"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(295..1317)
                     /locus_tag="VN968_11775"
     CDS             complement(295..1317)
                     /locus_tag="VN968_11775"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXL29953.1"
                     /translation="MPILLAPMAGACPASLSIAVANAGGLGACGVLLMQPDEINIWAR
                     DVRAGSNGSFQLNNWIPDPPPARDSVHEARVREFLGHWGPTVAPEAGEAATPDFAAQC
                     EAMLEADPRIISSIMGLYPEEFVAKMKARGIAWWATATTVAEARAAVAAGADVIVAQG
                     AEAGGHRGAFNAVEAESRQVGLIALLPAIVNAVDVPVVATGGIADARGVAAAFVLGAS
                     AAQIGTGFLRAPEAKIAPAWADALVRTAPEDTLVSRVFSGRPGRSIATAYARAATAPE
                     APAPAPYPIQRGLTAGMRGLGTKEDNIDRIQAWAGQSAALAAAESASAIVERSWTGAQ
                     ALLGLS"
     gene            1886..>3077
                     /locus_tag="VN968_11780"
     CDS             1886..>3077
                     /locus_tag="VN968_11780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXL29954.1"
                     /translation="MALDRPPIAAEIRATLAVAAPLAAANLAQMAMQVTNAVMVGHLG
                     AVPLAAAGLGNALNATLLMTSQGLLTAVAPLAAHSIGAGDHPAAGRVAGGGLIVATAV
                     AAPMMATLMVVPPLLSGLGYEPGLVAEIGRFLWAISWGIPGFLGAAVLRFLLIAAFRT
                     RIVMIVPLLAVPLNLVLNWVLIFGHWGMPALGSAGSGCATAIVQWLTLFSFALYMLIA
                     RARISVSMGRGVRRQIPGILRLGLPIAAMRGLEIGVFVMTGVMMGMIGADALAAHQLV
                     FNVAGVCFMVPLGLAQAATVRVAYQLGRSEPSAARRAAYVALAMGVLFMTAAALLLWT
                     MPHILIGFYLDLGDAADQSTVAIALQLFVIAALFQLFDGVQVIAAGALRGYRDTAVPM
                     LIAAI"
BASE COUNT          525 a         1020 c          980 g          552 t
ORIGIN      
        1 gcaaccatta cgccatttta tcccgtaagt tcaatgagtt atggatcagc agatccaact
       61 taaaatccgt agtcgataaa atcaatggct taagtgatgt aggttcaaat cacacgctac
      121 caacacactc atgccaccca ctgaatactc accaactgtc cgcttggaaa tctgcaacgt
      181 ccgctcggcc ttggcatcga gaactgctct tcatgcccca ttgccgtcat tgcccaaccc
      241 gaggcccgat ggccatgatg ccgctccctg gtgaggccag gcgggtggtt cccgctaaga
      301 cagacccaac agcgcctggg cccccgtcca cgatcgttca acgatggcgc tggccgattc
      361 ggcggcagcg agagcggccg attgaccggc ccaagcctga atccggtcga tgttatcctc
      421 cttggtccca aggccgcgca tcccggcggt caggccccgc tggatcgggt aaggcgcggg
      481 agctggcgcc tcgggggcgg tcgcagccct ggcgtaggcg gtggctatac tgcgaccggg
      541 acggccgcta aagacacggc tgacgagtgt atcctccggt gcggtcctta ccagtgcatc
      601 agcccaggcc ggagcgattt tcgcctcggg agcgcgcaaa aaacctgtac cgatctgtgc
      661 cgcgctggca ccgagcacga atgcagctgc gacgccgcgc gcatcggcga taccgccagt
      721 cgcgacaacc ggcacatcga ccgcattgac tatcgccggc agtagcgcga tgagcccgac
      781 ctgccgcgac tctgcctcga cagcgttaaa ggcaccacga tggccgccag cctcggcgcc
      841 ttgcgcgacg atcacgtccg cgccggcagc cacggcggca cgcgcctcgg ccacggttgt
      901 ggcggtcgcc caccacgcaa tcccgcgcgc cttcatcttg gcgacgaact cctcggggta
      961 gaggcccatg atcgacgaga tgatgcgcgg atcggcctca agcatcgcct cgcattgggc
     1021 ggcgaaatcg ggcgtggcag cctcacccgc ctccggtgcc accgtcgggc cccaatgccc
     1081 gagaaattcg cgcacccgcg cttcgtgtac cgaatcgcgc gcaggtggtg ggtcgggtat
     1141 ccaattattg agctgaaaac tgccgttgct gccggcccgc acatcgcgcg cccagatatt
     1201 gatctcgtcg ggctgcatca gcagcacgcc gcaagcaccg agcccgccgg cattagcaac
     1261 cgcaatagat agcgatgccg gacacgctcc cgccatcggt gccaacagga tcggcacgcg
     1321 cagctcgaac cgccggcaaa aagcttgagt tcgctcgaca atactgttca cagtgcccag
     1381 tcccgatcca attgatttct cgccacgcgc gctctctcga cgcgacgcga aagcttcagc
     1441 gtatcagaac acacaaaaag cggaatcggg aatcccccaa atggatcgcg gtttcaggcc
     1501 ctctgccaga gtcaacagac tgcgaagctc gcagcgcgcg aatggccgct gtcggctcta
     1561 ccccgccgtt ggctcatcag atctgaaggg ccggaatccc acccggtgaa gccattcgcg
     1621 gcccgtcgag accatcgacc gccctcaacc ccgagctaac ggctcaaacc gacccaagaa
     1681 cccgctctcg cgggatgacg cgcgtcgcgg tcggtgcatt ttcggcgata gtggtgagtg
     1741 ggtgagcggt agggcgtgac gaccggggca tccgatcccg agcattgaag gtccaacaac
     1801 cttcagctcg aaagctgggc cgcggtgacg gttggcgagc tggaagctgc aagagaggct
     1861 ggaatccagg taatgtccgc ggatcatggc tctcgatcgc ccgcccatcg ccgcggagat
     1921 ccgtgcgacg ctcgccgtcg cggcgccgct ggcggccgct aacctggcgc agatggcaat
     1981 gcaagtgacc aacgccgtga tggtcgggca tctcggcgcc gtaccgctag ccgctgccgg
     2041 gctcggcaac gcactcaatg cgacattgct gatgacctct caggggctgc tgacggcggt
     2101 agcgccactg gccgcgcatt cgatcggcgc cggcgaccac cccgccgcgg ggcgcgtcgc
     2161 cggtggcggg ctgatcgtcg cgacggcggt ggcagcgccg atgatggcga cgctgatggt
     2221 ggtgccgccg ctgctctccg gcctcggcta cgaacccggc ctggtggcgg agatcggccg
     2281 ttttttgtgg gcgatttcct ggggcattcc ggggttcctc ggtgcggcgg tgctgcgctt
     2341 cctgctgatt gcggcgtttc gcacccggat cgtcatgatc gtcccgctgc ttgcggtgcc
     2401 gctgaacctg gttctgaact gggtcctgat cttcggacat tgggggatgc cggccttggg
     2461 cagcgcgggc tcggggtgcg ccacggcaat cgtgcaatgg ctgacgctct tcagcttcgc
     2521 cctctacatg ctgatcgcca gggcaaggat atcggtcagc atgggtcgcg gcgtgcggcg
     2581 ccagatcccc ggcatcctgc ggcttggctt gccgatcgcg gccatgcgag ggctcgagat
     2641 cggggtgttc gtgatgaccg gcgtcatgat ggggatgatc ggcgccgatg cgctggcggc
     2701 gcatcagctg gtcttcaacg tcgctggcgt gtgtttcatg gtgcctctcg gcctcgccca
     2761 ggcggcgacc gtccgggtcg cctatcagct cggccgcagc gagccgtcgg cggcgcgccg
     2821 cgccgcgtat gtggcactgg cgatgggcgt tttgttcatg acggccgcgg cgctactgct
     2881 atggacaatg ccgcacatcc tcatcggctt ctacctcgat ctcggcgatg ccgccgacca
     2941 gagcacggtg gcgattgcgc tgcagctttt cgtgatcgcc gcactgttcc agctgttcga
     3001 cggggtgcag gtgattgcgg ccggggcgtt gcgcggctac cgcgacaccg ccgtcccgat
     3061 gctgatcgcc gcgatcg
//
LOCUS       DATNOI010000300         5046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1081944,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000300 DATNOI010000000
VERSION     DATNOI010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081944"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /locus_tag="VN968_11785"
     CDS             <1..114
                     /locus_tag="VN968_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL29955.1"
                     /translation="ASTAEDVAELVCFLATPRSSNMTGEVVRMDAGAHLIT"
     gene            complement(260..538)
                     /locus_tag="VN968_11790"
     CDS             complement(260..538)
                     /locus_tag="VN968_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HXL29956.1"
                     /translation="MSGILWVIIVGFVAGIIARLLSPGPNNPSGFILTTVLGIAGAFL
                     ATFIGQAIGHYGPDQGAGFITATIGALLVLFIWNRLVASRMISDPGNR"
     gene            complement(618..1193)
                     /locus_tag="VN968_11795"
     CDS             complement(618..1193)
                     /locus_tag="VN968_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HXL29957.1"
                     /translation="MGLLDVLNGMQNGPRGPSNPGAQTGGGMSPMTMAILALLAYKAV
                     KHLGGSQPTAAPAPAPSRGNTVNAALPGGGMGGGLGDLLKGGLGGLLAGGAAGSVISG
                     GLGDLLKQFQQNGQGDTANSWVSPGPNRAISPGDLAKALGADQIETLASQSGLSRDEL
                     LAGLSQHLPDVINHLTPDGRLPTESELSGRI"
     gene            1346..2356
                     /locus_tag="VN968_11800"
     CDS             1346..2356
                     /locus_tag="VN968_11800"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXL29958.1"
                     /translation="MILGRPIGVAGAGSIGCFVGGMFAACGRRVALLARPCVIQEIEG
                     NGLQLTSFEGLARNIASSALTLSEDPRILGDAGLVLVTVKSADTAGMADLIAGHAPAD
                     AVIVSLQNGVGNVAVLRQRLPGRRVLAGMVPFNVIALGNGRFHRATSGDIIIEQDDAG
                     TAQQLSVPGLKMRATDNIAGVQWGKLLLNLNNAINALADLPLRQQLKQRSWRMLFADQ
                     MAEGLAAIKAAGIKPVSSTPIPASWTPHLLRLPDAVFGVLLGRTMKIDPEARSSMWED
                     LQRGRRTEIDYLQGVITAIADRHGLHVPLSRRIVTLIRSAEVSGKGSPKLTPEQIRAD
                     LP"
     gene            2433..2681
                     /locus_tag="VN968_11805"
     CDS             2433..2681
                     /locus_tag="VN968_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29959.1"
                     /translation="MKRFYASVVVVVAVAVALGGWASVMPASAAPPTVTPSPGYDARL
                     QEQRAATARVIDEPVIRPAKRRHVSRPHVRRSYGGSVY"
     gene            complement(2768..3418)
                     /locus_tag="VN968_11810"
     CDS             complement(2768..3418)
                     /locus_tag="VN968_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29960.1"
                     /translation="MIASRQSGLLMAAVAACIALAPLRAHAQWWSTRAPVDFEDCADL
                     AEKAATKEARTSEIAACNAKFAGRRKSGGGYTYYDFMQNRSFDIAGPNPTKEEQKHID
                     EQYTVFLDKQRRSFIAASLAAKQDQQASVRTEKASLPVVAEAAPAKPKTTGADLKARA
                     KAKCAQHSTFSCEWPVLSEKITNLKKALFGSSQAKAKRGASSSRGEASGPVQSAAR"
     gene            3882..>5046
                     /locus_tag="VN968_11815"
     CDS             3882..>5046
                     /locus_tag="VN968_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXL29961.1"
                     /translation="MPKLHMTDVVVSRLKFSGTYYDETTPAFGLRVGKNRKTWFVVRG
                     RERLRTNIGQYPATSLADARKEARKLLVENPTNVGRITFNAAYDIYKEALKSKKERTQ
                     YDYKRVLEKYLQPKLGTKKLADISYDDITAITHPLAHSEKRNTLAVARTFFRWCVRPP
                     RRYLKHSPLEGVEIPKAGKRKRILDEKELVAVWQATVRQGYPHGTVCQLLALTGQREG
                     EIANLRRPWINEREQTITLPDWVTKNSKEHTFPYGSMVAEILEGIPRRNDTDLLFPCR
                     ISLERPISGWSKFKKELDDGVPNWTLHDFRRTYRSIHGQIGTSSDIAERLINHAAAVQ
                     TEVEAIYDRWHYLPQMRQAVLAYEEHFKALLARDRVRGPQYLTTGSAITGAMHM"
BASE COUNT          988 a         1543 c         1547 g          968 t
ORIGIN      
        1 gcgtcaaccg cggaagatgt cgccgaactg gtgtgcttcc tcgccacgcc acgctcgagc
       61 aacatgaccg gcgaggtggt tcggatggat gccggcgcgc atctcatcac gtaaaaatcg
      121 tagcccggat ggagccaacg ggtcggcgca aagcgccgcc cgatgacagg ctccgcgcaa
      181 tccgggaatc tcaaggcgag gttacgccgg ccccggattt cgcttcgctt catccgggct
      241 acaagtgcgc ccgtcggttt tacctgttgc ccgggtcgga aatcatccgg cttgccacca
      301 gcctgttcca gatgaacagc accagcagcg cgccgatggt cgcggtgatg aagccggcgc
      361 cctgatccgg accgtaatgg ccgatggcct ggccgataaa ggtcgcgaga aacgcgccgg
      421 cgatacctag cacggtggtc aggatgaatc cgctcgggtt gttcgggccc ggcgaaagca
      481 atcgcgcgat aattcccgcg acgaagccga cgatgatgac ccagagaatt ccgctcatgg
      541 ctgtcgtcct ctttttgaaa aagctaaagt tgcaatgatg atgcgacagg cgcgaccgcg
      601 tcgcgcctgt cgagacgtca gatccggcct gacaattcgc tttcggtcgg cagccgtccg
      661 tccggcgtca gatggttgat cacatcgggc agatgctggc tgaggcccgc gagcagttcg
      721 tcgcgcgaca gtccgctttg cgacgccagc gtctcgatct gatcggcgcc cagcgccttg
      781 gcgagatcac caggcgaaat cgccctgttc gggccggggc tcacccatga attggcggtg
      841 tcgccctggc cgttctgctg gaattgcttg agcaggtcgc cgagcccgcc gctgatcacg
      901 cttccagccg cgccgccggc aagcaggccg cccaatccac ccttcagaag atcgccaaga
      961 ccgccaccca ttccgccgcc gggcagggcg gcattcacgg tattgccgcg cgaaggcgcc
     1021 ggtgctggcg ccgcggtcgg ctggctgccg cccaaatgtt tgaccgcctt gtaggcgagc
     1081 agggccagga tcgccatggt catcggcgac atccctccgc ccgtttgcgc gcctggattg
     1141 ctcgggccgc gcgggccatt ctgcatgccg ttgagtacgt cgagtaaacc cattgttctc
     1201 tcctgccgca tttcctgccc cggaaagaca tagcggtgcg ctatgaccgt tacaaggcgc
     1261 agccgcgcca gccacggcgc cgtcggaatg gcaatccagc gccgaattct gctatcaaac
     1321 ccgggggata ttggtggaag gcgggatgat cttgggccga cctatcggcg tggccggggc
     1381 cggcagcatc ggctgctttg tcggcggcat gtttgcggcc tgcggccgcc gtgtcgcgct
     1441 gctggcgcgc ccgtgcgtga tccaggagat cgaaggtaac gggctccagc tgacgagctt
     1501 cgagggcttg gcgcggaaca tcgcatcgag cgcactgacg ctgtccgagg atcctcgaat
     1561 ccttggcgac gccggcctgg tgttggtgac cgtgaaaagc gcggataccg cagggatggc
     1621 ggatctcatc gcaggccatg cgcccgccga tgccgtcatc gtcagcctgc agaacggcgt
     1681 cggcaatgtc gcggtgctgc gccaacgatt gccggggcgt cgcgtgctgg cggggatggt
     1741 gccgttcaat gtgatcgcgc tcggcaatgg tcgatttcat cgcgccacgt cgggcgacat
     1801 cattatcgaa caggacgatg caggtacggc ccaacagctc tcggtgccag gcctgaaaat
     1861 gcgggcgacc gacaacatcg ccggcgtgca atggggcaag ctgctgctca atctcaacaa
     1921 cgcgatcaac gcgctggccg atctgccgct gcgccagcag ctcaagcagc gctcgtggcg
     1981 catgctgttc gcggaccaga tggcggaggg cctggctgcg atcaaggcgg cgggcatcaa
     2041 gccggtgtcg tcgacgccga ttccggcgag ctggacaccg catttgctgc ggctgcctga
     2101 cgcggtgttc ggggtgctgc tggggcgcac gatgaagatc gatcccgagg cgcgctcgtc
     2161 gatgtgggaa gacctgcagc gcggccgccg caccgaaatc gattatctgc aaggtgtcat
     2221 caccgccatc gccgacaggc acggcctgca cgtgccattg tcgcgccgga tcgtgacgct
     2281 gatcaggagt gccgaagtca gcggcaaggg ttcgcctaag ctcacgccgg agcaaatccg
     2341 cgctgacttg ccttgaaatc gcgatcatca gcagcgaatt tcgcatcagc ggccatgcgc
     2401 agatccgccg cgtcagcgcc gggaggaacg acatgaaacg attttacgca agcgtcgtcg
     2461 tcgtcgtcgc cgttgcagtt gcgctcggcg gctgggcttc ggtgatgccg gcaagtgccg
     2521 cgccgccgac cgtcacaccg tcaccgggat atgacgcgag gttgcaggag cagagggctg
     2581 cgacggcgcg ggtcattgat gaacccgtca tacgcccagc caaacggcgc catgtgagcc
     2641 ggcctcatgt gcgtcggagc tatggcggca gcgtttactg aaacgctgtt cacactgaga
     2701 gcgcgcgtcg gtgcgaaacc ggattctcgc gctggtcatc ggtggattgc agcttcgacc
     2761 gggcccgtca gcgcgccgcg ctctgcacgg gtccgctggc ctcgccacgg gaagacgagg
     2821 caccgcgttt ggctttggcc tgggacgagc caaacaatgc tttcttcaga ttcgtgatct
     2881 tctcggaaag caccggccac tcgcaggaga aggtggaatg ttgggcgcat ttcgctttgg
     2941 cgcgcgcctt gagatcggcc cccgtagttt ttggcttggc aggtgctgcc tccgcaacca
     3001 ccggcagcga cgccttttca gtccgcacgg aggcttgctg gtcctgcttt gccgcgagag
     3061 acgccgcgat gaagctccgc cgctgcttgt cgagaaagac ggtgtattgt tcgtcgatgt
     3121 gcttttgttc ttctttcgtc ggattgggac ccgcgatatc gaagctgcgg ttctgcatga
     3181 agtcgtaata agtgtagccg ccgccggact tgcgccgacc ggcaaatttc gcgttgcatg
     3241 cggcgatttc cgacgtccgc gcttccttgg tcgccgcctt ctccgcgagg tcggcgcagt
     3301 cctcgaaatc gaccggtgcg cgcgtgctcc accactgcgc atgcgcgcgc aacggcgcca
     3361 gcgcaatgca tgcggcgacc gcggccatca acagtccgga ttggcgcgat gcgatcacgg
     3421 acgattttcc caacacaact ctcaagacgc gactcacaca acatgatttg cgcggattgt
     3481 cgccattatt cggcggcttg tcaccccgga accgggtttt tgtacggcac gagccgcgcg
     3541 ctctttcggt cctgtgacaa gcgcgacaca tctgcggcta gttgcaaccg accgggattc
     3601 aatcccaatg cgatcatatt gggatggtgc gcaggctggc gatcaccacg gcgctctggt
     3661 atggagtttt caagcgcccg cgtccacccg cggggcttcg gtcccgtagc tcagctggat
     3721 agagcgacgg tttcctaaaa cgaaatcgcg ctcacccacg gttcaccgcg gttctccatt
     3781 tctgaaatca ttccaataac ttaagccctg cgcttctgct acagtgctcc cctgatatcc
     3841 gcctctatcc aggggattgc ggagcaaaat cggagcaaaa aatgccgaag ctgcacatga
     3901 cagacgttgt tgtgtcgcgg cttaagttca gcggcaccta ttatgatgaa acgactccgg
     3961 cattcggtct gcgcgtcggc aaaaatcgaa agacatggtt cgttgtccgc ggccgagagc
     4021 ggctgcgcac gaacatcgga caatatccgg cgacatcact cgccgacgct cggaaggaag
     4081 ctcgcaaact tcttgtcgaa aatccgacga atgtgggccg gatcacattc aacgcggcgt
     4141 atgatatcta caaagaagcc ttgaagagca aaaaagagcg gacccaatac gattacaaac
     4201 gggtcttgga gaaatatctg cagccgaagc ttggcaccaa gaaacttgcc gacatttcct
     4261 acgacgatat caccgcgatc actcatccgc ttgctcatag tgagaagcgg aacacattag
     4321 ctgtcgctcg cacgttcttc cgttggtgcg tccggccacc cagaagatac ctcaagcact
     4381 caccgcttga gggtgtcgag attccgaaag ctggcaaacg gaaacgaata ctcgacgaga
     4441 aggagctcgt tgccgtttgg caagcaacgg ttcgccaggg ctatcctcac gggacggtct
     4501 gtcagttgct tgctcttacg gggcagagag aaggcgaaat cgcaaacctg cggcggcctt
     4561 ggatcaacga gagagagcag acgattacac ttcccgactg ggtcaccaaa aactcaaaag
     4621 agcacacatt tccctacggc agcatggtcg cggaaatctt agaaggcata ccgcgccgca
     4681 acgatactga cttactcttc ccgtgccgca tttctcttga acggccgatc tcaggctgga
     4741 gcaagttcaa gaaggagttg gacgacggcg taccgaattg gacactgcat gactttcgca
     4801 ggacgtaccg gagcattcac ggccagattg gtacgtcctc cgatatcgct gagcggctca
     4861 ttaatcacgc cgcggctgtc cagacagaag tcgaagcgat ctacgatcgg tggcactact
     4921 tgccgcaaat gagacaagca gttctcgcgt acgaagaaca cttcaaagcc ctgctcgctc
     4981 gtgatcgcgt ccgcggcccg cagtatctaa ccaccggttc ggctattacc ggggcgatgc
     5041 atatgc
//
LOCUS       DATNOI010000301         2755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_368929,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000301 DATNOI010000000
VERSION     DATNOI010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368929"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1311)
                     /locus_tag="VN968_11820"
     CDS             complement(19..1311)
                     /locus_tag="VN968_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HXL29962.1"
                     /translation="MVVGSDVCLRRLAKGNRGREVRFNRFLGNAKVTTARIIESWSEG
                     TVAAADGRHILAIQDTSEINFATTARRRRGLGEIGKGNGHGVLLHSMLAVDAENGSCL
                     GLLGGEVWTRKGRRTISHDLRDLSDKESQRWITTALAAKPLLAKAAMMTVIGDRESDI
                     FALYANAAEQHFHVIARSMHDRKLANGTGLYAASETMAVVDRRAIVLPARAQRAERVA
                     VLELRFGAVNLARPQTRFLHHLPESLPLTLVDVREIDPQAGTEPLHWRLLTDHTVANA
                     EQAWRIVEWYKRRWLIEQFHRVLKTQGFKLEDSQIATADRLLKLVAIAAKAAIITIQL
                     LQARDGRSHQPVRLAFDANEVATLAALNQNLEAQSKRLRNPHPPDSLAWAAWIIGRLG
                     GWDGYPSSKPPGPITLKHGLEYFYAVAVGRSLKDVCML"
     gene            complement(1529..>2755)
                     /gene="purF"
                     /locus_tag="VN968_11825"
     CDS             complement(1529..>2755)
                     /gene="purF"
                     /locus_tag="VN968_11825"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXL29963.1"
                     /translation="LPGNAAIGHVRYSTTGATILRNVQPLFAELNAGGFAVGHNGNLT
                     NGLTLRRDLVRNGAMMQSTTDTEVILHLVAQSKRNRFIDRFIEALRAIEGAYSLVSLT
                     NKKLIGARDPLGIRPLVLGELDGCPILASETCALDIIGAKYVRDVEPGEVIVFDERGA
                     QSHKPFPPKPPRPCIFEYIYFSRPDSIVGGRSVYEVRKAFGAQLARESHVEVDVVVPV
                     PDSGVPAAVGYSQHSGVPFELGIIRNHYVGRTFIQPTQSVRELGVRMKHNANRAAIEG
                     KRIILIDDSLVRGTTSKKIVRMMRDAGAREVHFRLASPPIVHPDYYGIDLPDRGGLLA
                     ATHNLEEMREIIGADSLAFLSIDGMYRAMGEPGRDPLNPKFADHCFTGAYPTHLTDQT
                     QVEPQPRQLSLLAEAS"
BASE COUNT          495 a          850 c          894 g          516 t
ORIGIN      
        1 tgggtccccg ctttcgcgct agagcatgca cacatctttg aggctccgtc cgacagcgac
       61 agcgtagaag tattcgaggc cgtgcttcag ggtgatgggg cctggcggtt tggaggaggg
      121 atagccgtcc cagccgccaa ggcggccgat gatccaggcg gcccaggcga ggctgtcggg
      181 cggatgcggg ttcctaagcc gtttactttg ggcttcgaga ttttgattga gggcggcgag
      241 tgtggcgacc tcgttggcat cgaaggcaag ccggacgggc tgatgactac ggccgtcgcg
      301 cgcctgcaaa agctggatgg tgatgattgc cgccttggca gcgatcgcga ccagcttgag
      361 gagtcgatcg gcggtagcga tctggctgtc ttccagcttg aagccttgcg tcttgagcac
      421 gcggtggaac tgctcgatca gccagcgccg cttgtaccat tcgacgatgc gccaggcctg
      481 ttctgcattc gccaccgtgt ggtcggtaag aagccgccag tgcagcggct ctgtgccggc
      541 ctgaggatcg atctcacgca catcgaccag ggtcagcggc aagctttcag gcagatggtg
      601 caggaatctg gtttgcggcc gggccaggtt caccgcgccg aagcgcagtt cgagaactgc
      661 gacacgttcg gcccgctgcg cacgcgcagg cagcacaatg gcgcgccggt ccacgacggc
      721 catggtctca ctagccgcat acaagccggt gccgttcgcg agtttgcgat catgcatgct
      781 gcgggcgatc acatggaagt gctgttccgc cgcattggca taaagggcaa agatgtcgct
      841 ctcgcgatcg ccgatcacag tcatcatggc ggccttggca agcagcggct tggccgccag
      901 cgccgtggtg atccagcgct gcgattcctt gtccgacaag tcgcgcagat cgtgcgagat
      961 ggtgcggcgc cccttgcgcg tccatacctc accgcccaac aggccaagac agctgccgtt
     1021 ctcggcgtcc accgccagca tcgagtgcag caacaccccg tggccgttgc ccttgccgat
     1081 ctccccgagc ccgcgccggc gtcttgccgt ggttgcgaag ttgatttcgc tggtgtcctg
     1141 gatcgccagg atatggcggc catcggccgc cgccaccgtg ccttcgctcc agctttcgat
     1201 gatacgggcc gttgtcacct tggcattgcc gagaaagcgg ttgaaccgca cttcccggcc
     1261 gcgattgcct tttgccagtc gccgcaagca gacatcactg cccacaacca tgcattcgat
     1321 gagcgccgcc cccctttatc gagacgacga tccccgaacc gacccaatga ccagtcaatt
     1381 tgccccaaca tgtcgacacc tctgccaaag ccgaagtgcc gcaatagaaa tcacacaaat
     1441 tcccgattct ggaatccccc cggtcagacc cgagtcaata cgtcgcacca gatgtgtgca
     1501 tgccctagcg ctttcgcggg gacgacaact agctcgcttc cgccaacagc gacaattgcc
     1561 gcggctgcgg ctcgacctgg gtctgatcgg tgagatgggt gggataggcg ccggtgaagc
     1621 aatggtccgc gaatttcgga tttaagggat cgcggcccgg ctcgcccatg gcgcgataca
     1681 tgccgtcgat cgacaggaac gccagtgaat cggcgccgat gatctcgcgc atctcctcga
     1741 gattgtgggt cgcagccaac agtccgccac ggtccggcag atcgatgccg tagtaatcgg
     1801 gatgaacgat tggcggtgag gcaaggcgga aatgcacctc gcgggcgccg gcgtcgcgca
     1861 tcatgcggac gatctttttc gaggtggtcc cgcgcaccag cgaatcgtcg atcaggatga
     1921 tgcgcttgcc ttcgatcgcc gcgcggttcg cattatgctt catgcgcacg ccgagttcgc
     1981 gcacgctctg ggtcggctga atgaaggttc ggccgacgta atggtttcgg atgatgccga
     2041 gttcgaacgg cacgccggaa tgttggctgt agccgaccgc ggcgggtacg ccggaatccg
     2101 gcaccggcac cacgacgtcg acctcgacat gactttcacg cgcgagctgc gcgccaaacg
     2161 ccttgcggac ttcatacacg gatcgcccgc caacgatgga atccggccgc gaaaagtaga
     2221 tgtactcgaa gatgcagggc cgcggcggct ttggcggaaa cggcttgtgg ctttgcgcgc
     2281 cgcgctcgtc gaacacgatg acctcgccgg gctcgacgtc gcggacgtat ttcgcgccga
     2341 tgatgtcgag cgcgcaggtt tccgaggcga ggatcggaca gccgtcgagt tcgcccagca
     2401 ccagcggacg gatgccgagc ggatcgcgcg ccccgatcag cttcttgttg gtcagcgaca
     2461 ccagcgaata ggcgccctcg atcgcgcgca gcgcctcgat gaagcggtcg atgaagcggt
     2521 tgcgcttgga ctgcgcgacg aggtgcagga tgacttcggt atcggtggtg gactgcatca
     2581 tggcgccgtt gcgcacgaga tcccggcgca gcgtcagccc gttggtgaga ttgccgttgt
     2641 ggccgaccgc gaaaccgccg gcattgagtt cggcgaacag cggctgcacg ttgcgcagga
     2701 tggtggcgcc ggtggtcgag tagcggacat ggccgatcgc ggcattgccg ggcag
//
LOCUS       DATNOI010000302         3778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1965611,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000302 DATNOI010000000
VERSION     DATNOI010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965611"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1054
                     /locus_tag="VN968_11830"
     CDS             110..1054
                     /locus_tag="VN968_11830"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HXL29964.1"
                     /translation="MTVGVGGSTAEAELAAMNNMRGDVPPIGVDERLRRIARAQAIMR
                     EKGIDALYLDVSSSMTYFTGLKFRRTERMHSAVLPAKGEIVYVSPAFEVEKLRTMIAF
                     GDKIAVWEEDEDPTATVTETVRALGYPSGTIAVDEATPFFTFDGLRRAGNAYTFINSI
                     DVTAGCRMIKSDHELALMQTAKNITLEAHRAAARIMREGITTTEVQAFITAAHRKLGS
                     EGDPPFNAVLFGEASAYPHGVPYPQTLKDGDMILIDTGAPVDGYLSDITRSYVFGEPT
                     RRQRDVWNLEKAAQAAGFAAAKPGQPLRGNRHRRPRGH"
     gene            1050..1328
                     /locus_tag="VN968_11835"
     CDS             1050..1328
                     /locus_tag="VN968_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HXL29965.1"
                     /translation="MNGAGFGPGYATPGLPHRTGHGIGLDVHEWPYLVKGDKTVLKPG
                     MTFSNEPTICIYGEFGVRLEDHMVITQTGARWFTQPAHSVDDPFGLEK"
     gene            complement(1349..2287)
                     /locus_tag="VN968_11840"
     CDS             complement(1349..2287)
                     /locus_tag="VN968_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HXL29966.1"
                     /translation="MIGVLIFPDFQLLDAAGPISVFEIAMRFAGGPPSIKVLAATPGP
                     VRSSSGVELLARGLKPIGAISTLIVAGGEGVDAAAKCDKTLSFVRAIAKRGVRVASVC
                     SGAYMLAEAGLLDGRRATTHWGRTRHFLSAHPRVKWEPDQIFVRDGNIWSSAGITAGI
                     DLALAMAAEDFGDEIAQKTARQLVLYHRRSGGQSQFSSLLELKAPSGRFGPLLTWARE
                     HLDAPLTVEDLAEQAGMSSRHFARAFIAETGTTPSKAVERLRIEVARARVQSSSEAIE
                     RVAQSTGFRDPGRMRRAFIRAFGQPPQSLRRAARAG"
     gene            2531..3238
                     /locus_tag="VN968_11845"
     CDS             2531..3238
                     /locus_tag="VN968_11845"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HXL29967.1"
                     /translation="MSAPLQIGLVLFPRVTQLDFTGPLQVFSSLPGAKVHLIWKRIEP
                     VASDSVLMLTPTVTLADCPHLDVICVPGGVGTDDMVNDDEMLDFLRRQAKGAKYVTSV
                     CTGSLVLGAAGLLQGYRATTHWSAMDHLAPLGATPTRTRVCVDRNRVTGGGVTAGIDF
                     ALTLVSLLVDRQTAEAIQLRLEYNPAPPFNAGSPDTAPPEVLALMKAKIAPFQQRRGE
                     AVQRAAARLATQDVAAT"
     gene            complement(3593..>3778)
                     /locus_tag="VN968_11850"
     CDS             complement(3593..>3778)
                     /locus_tag="VN968_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HXL29968.1"
                     /translation="ADGGQRVLFYSGNDTAAKAEVAALIDRIGFVGIDLGSLAVGGKL
                     AQFPGGPLPNQNLIKIG"
BASE COUNT          697 a         1262 c         1190 g          629 t
ORIGIN      
        1 ggcgcttccg tcgcgttcgt gctgatggcc gcaacgctgg ggctgacggc gacggcaaat
       61 atcctggtac agcgccggtt ccggcgatga cgattgaaag gggcttgtga tgacggttgg
      121 cgtcggcgga tcgacggcgg aggcggaact tgccgccatg aacaacatgc gcggcgacgt
      181 gccaccgatc ggcgtcgatg agcggctgcg gcgcatcgcc cgcgcccagg cgatcatgcg
      241 cgagaagggc atcgacgccc tctatctcga tgtctcctcg agcatgacct attttaccgg
      301 attgaagttc cgccgcaccg aacgcatgca cagcgcggtt ctgccggcca aaggcgagat
      361 cgtctatgtc tcgccggcat tcgaggtcga gaaactcagg accatgatcg cgttcggcga
      421 caagatcgcg gtctgggagg aggacgagga tccgaccgca accgtcaccg agaccgtgcg
      481 cgcactcggc tatccaagcg gcaccatcgc cgtcgacgag gccacgccgt ttttcacgtt
      541 cgacggactg cgccgcgccg gcaacgccta caccttcatc aactcgatcg acgtcaccgc
      601 cggttgccgc atgatcaagt ccgatcacga actggcgctg atgcagacgg ccaagaacat
      661 cacgctcgaa gcccacaggg ccgccgctcg catcatgcgc gaaggtatca ccaccaccga
      721 agtgcaggcc ttcatcaccg ctgcgcaccg caagctcggt tcggaaggcg atccgccttt
      781 caacgcggtg ctgttcggcg aggcgtcggc atatccgcac ggcgtgccct atccgcagac
      841 gctcaaggac ggcgacatga tcctgatcga caccggcgcg ccggtcgatg gctatctctc
      901 cgatatcacg cgctcctacg tcttcggcga accgaccagg cgccagcgcg acgtctggaa
      961 cctggagaaa gcggcgcagg ccgcgggctt cgccgccgcc aaacccgggc aaccgctgcg
     1021 aggaaatcga catcgccgcc cgcgcggtca ttaacggcgc cggtttcggg ccgggctatg
     1081 cgacacccgg cctgccgcac cgcaccggcc acggcatcgg cctcgacgtg catgaatggc
     1141 cctatctcgt gaagggcgac aagaccgtgc tgaagcccgg catgaccttc tcgaacgagc
     1201 cgaccatctg catctacggc gaattcggtg tgcgcctcga ggaccacatg gtcatcaccc
     1261 aaaccggcgc ccgctggttt acccagccgg cccattcggt cgacgatccc ttcggcctgg
     1321 agaagtaagg acgcaaattg ccttgggcct accccgcccg cgccgcgcgg cgcagcgatt
     1381 gcggcggctg gccgaaggcg cggatgaagg cgcggcgcat ccgccccgga tcgcgaaagc
     1441 cggtggattg cgcgacgcgt tcgatcgcct cgctggagga ttgcacgcgc gcccgcgcca
     1501 cctcgatccg cagccgctcc accgccttcg atggcgtggt gccggtttcg gcgatgaagg
     1561 cgcgggcgaa atgccgcgaa ctcatgccgg cctgctcggc caggtcctcg acggtgagcg
     1621 gcgcatcgag atgctcgcgc gcccaggtca acagcggtcc gaagcgcccg ctcggtgcct
     1681 tcaattccaa tagcgaggag aactgcgact ggccgccgct gcggcggtga tagagcacga
     1741 gctggcgcgc ggtcttctgc gcgatctcgt cgccgaaatc ctccgccgcc atcgccagcg
     1801 ccagatcgat gccggcggtg atccccgccg aactccagat gttgccgtcg cggacgaaga
     1861 tctggtcggg ctcccacttc acccggggat gggccgagag aaagtgccgg gtgcgtcccc
     1921 aatgcgtggt ggcgcggcgg ccatcgagca ggcctgcctc ggcaagcata taggcgcccg
     1981 agcacacgct ggcgacgcgg acgccgcgtt ttgcgatagc gcggacgaag ctcagcgtct
     2041 tgtcgcactt tgccgcggcg tcgaccccct cgcctccggc gacgatcagg gtagagatcg
     2101 cgccgatcgg cttaagaccg cgcgcgagca attcgacgcc cgatgaactg cgcaccggac
     2161 ccggcgtcgc cgccagcacc ttgatcgacg gcggaccgcc tgcgaagcgc atggcgattt
     2221 cgaacaccga aatcgggccg gcggcgtcca gcaactggaa atcgggaaag atgagcacgc
     2281 cgatcatgat ttaacgtgat gtcctaaaat gagggaaata cgccatttcg gacagaagac
     2341 gatcatggca ggatccttac gtcaagtagc cggttggcct tcggcctcca catacccggc
     2401 cgtcatgccc ggacttgatc cgggcatcca tcatcttcga aagactctta gcgaagagga
     2461 tggattgccg ggtcaagccc ggcaatgacg ggttgctggt tagatcgcaa aacttcgaaa
     2521 ggctctcagc atgtcggcgc cgcttcagat tggtcttgtg ctatttccaa gagtgaccca
     2581 gctcgacttc accggaccgc tgcaggtgtt ctcaagcctg cccggcgcga aggtgcatct
     2641 gatctggaag cggatcgagc cggtcgccag cgactcggtg ctgatgctga cgccgaccgt
     2701 caccttggcg gactgcccgc acctcgacgt gatctgcgtt cccggtggcg tcggcaccga
     2761 cgacatggtc aacgacgacg agatgctgga tttcctgcgc cggcaggcca agggagccaa
     2821 gtacgtcacc tcggtctgca cgggatcgct ggtgctgggg gccgccggtc tgctgcaagg
     2881 ttaccgcgcg accacgcact ggtcggcgat ggatcatctt gcgccattgg gcgcgacgcc
     2941 gaccaggacg cgggtgtgcg tcgaccgcaa ccgcgtcacc ggcggcggcg ttaccgctgg
     3001 catcgatttt gcgctgacgc tggtgtcgct gctggtcgac cgccagaccg cggaggcgat
     3061 ccagctcagg ctcgaataca atcccgcgcc gccgttcaac gccggatcgc ccgacaccgc
     3121 gccgccggaa gtcctggcgt tgatgaaagc gaagatcgcg cctttccagc agcgccgcgg
     3181 cgaggcggtc caacgcgccg ccgcgcggct cgcaacacaa gacgtagcgg ccacttagcg
     3241 gctcgcttcc tcttgcaaaa aatgcgcgcc aagaaaaagg ccgcccggtt tcccagacgg
     3301 cctttcctgt cctacggcgg caccacattc acgtgcgatt tcaggacatc tgcgccaggg
     3361 gtctgctcca agccggatcc tgatcgggtg ccgctgcaca tcggggcagt cgcgaactgc
     3421 agcccgaacc gtcgcgatgg tctcccatct ccgtaagatg actgattgag ccacgatggc
     3481 gtccactgtt caagaacgcg gtgacaacta tccccgctgt tcccgctgtt cacagacggc
     3541 tttccgggcc ggacatcgcc aaggtccgaa aagtgcctgc caaaaggcga catcagccga
     3601 tcttgatcag attctggttc ggcagtggtc cgccggggaa ctgagcgagc ttgccgccga
     3661 ccgcgagcga tcccagatcg atgccgacga agccgatcct gtcgatgagc gccgctactt
     3721 cagcctttgc tgcggtgtcg ttgccggaat agaaaagcac gcgctgcccg ccgtccgc
//
LOCUS       DATNOI010000303         7806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_371382,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000303 DATNOI010000000
VERSION     DATNOI010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371382"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VN968_11855"
     CDS             complement(<1..329)
                     /locus_tag="VN968_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29969.1"
                     /translation="MTHYHNPAWTEESRFRGALDDVIIIPLVFVALAANKILRFILSI
                     LMRLLDYAFPLAMQIVWLLLFAVRVLGNVIVIVINGALRFIPLSETKRRQWSKSIRRN
                     WSWLRRK"
     gene            complement(531..806)
                     /locus_tag="VN968_11860"
     CDS             complement(531..806)
                     /locus_tag="VN968_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HXL29970.1"
                     /translation="MRKDIKASCQKRLSRIEGQVRGLSKMVDEDRYCIDIVTQISAVR
                     AALRRVEEEVLRDHVAHCVEGAIASGNKSDQRQKISELMDVIGRSDR"
     gene            863..3103
                     /locus_tag="VN968_11865"
     CDS             863..3103
                     /locus_tag="VN968_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HXL29971.1"
                     /translation="MIKAENANIGGASKDSGCCGGGHNHSGHDHDSHHANPKAAVPEG
                     TIYTCPMHPQIRQIGPGHCPICGMALEPELASLDAPPNPKLADMTRRFWVGLALSLPA
                     VVLEMGGHLAGGHGLIDQTLSNWIQLAFATPVVLWAGWPFFVRGWQSLVTRNLNMFTL
                     IAMGTGVAYVYSLVGTVAPDIFPATFRGHDGAVAVYFEAAAVITVLVLLGQVLELRAR
                     EATSGAIKALLELAPKTARVVDANGADHEVPIDSLKVGDKLRVRPGEKVPVDGVMLEG
                     RSTLDESLVTGESMPVAKEPGAKVIAGTLNQSGGFVMRADKVGRDTLLSQIVKMVADA
                     QRSRAPIQRLADRVAGWFVPTVIIVAVIAFGAWSLSGPEPRMAYGLVAAVNVLIIACP
                     CALGLATPMSIMVGVGRGAQAGVLIKNAEALERMEKVDTLVVDKTGTLTEGRPKVVAI
                     VTTSGFQESEILRLAASVERASEHPLADAIVRAAKERNLDLSKVEEFDSPTGKGATGK
                     VDGKTVVLGNSNFFTSLGIETQPLNEQGERLREDGATVINIAVDGRLAGLFAIADPIK
                     PSTPDALKALSAEGIKVIMLTGDNRTTANAVARKLGISDVEAEVLPDQKIAVVTKLQK
                     AGRIVAMAGDGVNDAPALAAADVGIAMGTGTDVAMESAGITLLKGDLGGIVRARRLSQ
                     ATMRNIRQNLFFAFVYNAAGIPIAAGILYPHFGILLSPIIAAAAMALSSVSVVGNALR
                     LRVTPL"
     gene            complement(3366..4313)
                     /locus_tag="VN968_11870"
     CDS             complement(3366..4313)
                     /locus_tag="VN968_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HXL29972.1"
                     /translation="MTRLQDVYPDDRVSLREVGLRDGLQLVKTFPSTAAKQRWIRDEY
                     GAGVRHFEVGSFLPARTFPQFADVREVVRTVAALPGAHGIALALNERGVNEALASGVA
                     EIASVVSATEEHSQANANRSRDQAIANVKRLCELRDASPHKSIVNAAISMALGCSITG
                     PVEPREVMKLAEKCLEAGVDFVAIADTVGYAGPKQVGELTRAIVKMSGAKPVCIHLHD
                     TRGMGIANASAALDAGARILDGSLGGLGGCPFAPGATGNVVFEDLVFLCESKGFATGI
                     DIDKLVAVRSILRSEMPGEPLYGGLARAGLPGRTSAAAA"
     gene            complement(4310..5488)
                     /locus_tag="VN968_11875"
     CDS             complement(4310..5488)
                     /locus_tag="VN968_11875"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXL29973.1"
                     /translation="MSTQGLPLSGVRVIEMTHMVMGPTCGMILAQLGAEVIKVEPPAG
                     DKTRSLGGMGTSFFPLFNRGKRSVVLDFTRPEDCETMDRLLASADVFLENFRDGQLEK
                     QGLGADELRRKYPHLIVAGHKGFLSGPYEHRPALDEVVQMMSGLAAMTGTMDKPQRVG
                     SSANDIMGGMFGAIAILAALYQKRGGAAGADIRIGLFENCLFLVAQHMVEYEMTGIKP
                     RSMPEREHAWPIYDIFETAGGERIFIGVVTEGHWQSFCREFGLHDFLDDPALRTTTDR
                     ILARAKIIPHVAEVIKRWNSADLSATLDRLNICFSPINRPEDLFDDPHVLRPGGLVNN
                     TNANGEAFRVPALPIEWNGASIGEGLKVPVLGADTSALRSELEAQEFSPTGKTAVRLA
                     "
     gene            5720..6472
                     /locus_tag="VN968_11880"
     CDS             5720..6472
                     /locus_tag="VN968_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter family protein"
                     /protein_id="HXL29974.1"
                     /translation="MLDAAVKALSQILSPPMRSILWRSIGLALVLIVVLAVGLQRLLS
                     WFAVYGEDWAEAMLGPGFHTPLNILAWIVSIAAGLGVVLGAVFLMPAITSLVASVFVD
                     EVADHVEREHYPAERPGTALPFGLAMTEGIKTALLTILVYLIALPFVFVAGAGFIAFF
                     IATAWLLGREYFELAAMRFRPPAEAKAMRKQNAAIVFTAGLVIAAFVSIPIVNLATPL
                     FGMAFMVHMHKRLSGPRPELIERERKTSVPVG"
     gene            complement(6567..7376)
                     /gene="nth"
                     /locus_tag="VN968_11885"
     CDS             complement(6567..7376)
                     /gene="nth"
                     /locus_tag="VN968_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXL29975.1"
                     /translation="MAKITRKPLTKSKPTPPQRVKKPRHRPAAGRARKKSAKKPATNP
                     GQARAAKKSLKAIKPWTPAEVYEAFSRFRKANPEPKGELEHLNPYTLLVAVVLSAQAT
                     DAGVNKATRELFRVADTPQKMLALGEERLRDYIKTIGLYRNKAKNVIALSEKLIAGFG
                     GEVPRTRTELESLPGAGRKTANVVLNMAYGEHTLAVDTHVFRVANRTGLAPGNTPLEV
                     ELGLERVVPAEFKLHAHHWLILHGRYTCLARKPRCEVCLINDLCRWPEKTV"
     gene            7399..>7806
                     /locus_tag="VN968_11890"
     CDS             7399..>7806
                     /locus_tag="VN968_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HXL29976.1"
                     /translation="MTAGNDFDPGLAQTELFSALLTPHRSLNRTGFLVLMVFLSAVSF
                     AAGLAFLLMGAWPVLGFFGLDALAIYWAFRVNFLRARASEEISITPSELRVRLVSHRG
                     HVVEWVLNPLWVQLDQKTHAEFGIEKLYLVSRGR"
BASE COUNT         1398 a         2426 c         2464 g         1518 t
ORIGIN      
        1 atcttccgcc ggagccacga ccagttccgg cggatcgatt tactccactg tcgacgtttc
       61 gtctcggaaa ggggaatgaa gcgcaacgcg ccgtttatga cgataacgat gacattgcca
      121 agaaccctga ccgcgaagag cagaagccag acaatctgca ttgctaaagg gaaggcgtag
      181 tcgagcaacc gcatcaagat cgagagaatg aaacgcagga ttttgtttgc ggcgagcgcg
      241 acaaaaacaa gtgggatgat tatcacatcg tcgagggcac cgcggaagcg gctttcctcg
      301 gtccatgccg ggttgtgata atgggtcacg gtgcaacggt tccgggctga agacagggat
      361 cgtgccgctg caaattagca cactgtaagg gccatggggt ggcctcaggt accggtcgtt
      421 ggagcgacag cccgatgttc gctaacggtc aaagccggaa aagctcgcca cgagcacatg
      481 ttttcggctt tacccggtgg cggacctggg tgcactgcgg cgagccaaga ttacctgtcg
      541 gatcggccaa tgacgtccat cagctcggaa atcttctggc gctgatcgga cttgttgccg
      601 ctggcgatcg ccccttcgac gcaatgcgcc acgtgatctc tcagcacttc ttcctcgacg
      661 cgccgaagcg cggcgcggac ggccgagatc tgcgtgacga tatcgatgca atagcggtcc
      721 tcatcaacca tttttgaaag gccgcgaacc tggccctcga tccggctcag gcgtttttga
      781 caggatgcct tgatgtcttt tctcatgcgg cctatatacc cctacagggt atgggttgca
      841 agtgtccgtg gtggagagtt cgatgatcaa ggccgagaac gcgaatatag gcggcgcttc
      901 aaaagattcc gggtgctgcg gcggaggcca caatcactcc ggccatgatc atgacagcca
      961 tcatgccaac cccaaggccg ccgtccccga aggcacgatt tatacctgtc cgatgcatcc
     1021 gcagatccgc cagatcggtc ccggacactg cccgatctgc ggcatggcgc tggagcccga
     1081 actggcatcg ctggacgcgc cgccaaatcc caaactcgcg gacatgacgc ggcgcttctg
     1141 ggtcggcctt gcgctttccc ttccggcggt cgtcctggag atgggcggtc atcttgccgg
     1201 aggtcacggc ttgatcgacc aaaccctgtc gaactggatt cagctggcgt tcgccacccc
     1261 tgtcgtgctg tgggccggat ggccgttttt cgtgcgcggc tggcagtcgc ttgtgacgcg
     1321 caatctcaac atgttcaccc tgatcgcgat gggcaccggc gtggcctatg tctacagcct
     1381 cgtcggcacc gtcgcgcccg atatttttcc ggcgactttt cgcgggcatg acggcgcggt
     1441 tgcggtctac ttcgaagccg ccgccgttat caccgtgctc gtcctgctcg gccaggttct
     1501 cgagctgcgc gcccgcgagg cgacgtccgg agcgatcaag gcgctgctcg agctggcgcc
     1561 gaaaaccgcc cgtgttgtcg atgcgaatgg cgccgaccat gaggttccga tcgatagcct
     1621 gaaggtcggc gacaaactgc gcgtccggcc gggagaaaag gtgcccgtgg acggcgttat
     1681 gctcgaggga cgatcgaccc tcgatgaatc cctggtgacc ggtgaatcca tgcctgtcgc
     1741 caaggagccc ggcgccaagg tgattgccgg cacgctcaac caatccggtg gctttgtgat
     1801 gcgcgccgac aaggtcgggc gcgataccct gctttcgcag atcgtaaaaa tggtcgccga
     1861 cgcgcagcgc tcgcgcgcgc cgatccagcg gctcgccgat cgggtcgccg gctggttcgt
     1921 gccgaccgtg atcatcgttg cggtcatcgc ctttggcgcg tggtccctgt ccgggccgga
     1981 gccgcggatg gcgtacggcc tggtggcggc cgtcaatgtg ctgatcatcg cctgcccctg
     2041 cgcgctcggc cttgccacgc cgatgtcgat catggttggc gtcgggcgcg gggcgcaggc
     2101 tggcgtactg atcaagaatg ccgaagcgct ggagcgcatg gaaaaggtcg acaccctggt
     2161 ggtcgacaag acggggacgc tgaccgaagg caggccgaag gtcgtcgcga tcgttacaac
     2221 gagcggattt caggagagcg agattctgcg gcttgcagcc agtgtcgaac gcgccagcga
     2281 gcacccgctc gcggacgcga tcgtgcgggc ggcgaaagag cgcaatctcg atttgagcaa
     2341 ggtcgaggag ttcgattcgc cgaccggcaa gggcgcaacc ggcaaggtcg atggcaagac
     2401 cgttgttctc ggcaattcga attttttcac atcgctcgga atagaaacgc agccgctgaa
     2461 tgagcagggc gagcgtctgc gcgaagacgg cgccaccgtc atcaacatcg cggtcgacgg
     2521 caggctggcc gggctgtttg cgattgccga tccgataaaa ccgtcgacgc cggatgcgtt
     2581 aaaggcgctg tcggccgaag gcatcaaggt catcatgctg accggggaca accgcaccac
     2641 ggccaacgcc gtcgcgcgaa aactcgggat atcagatgtc gaggcggaag tgcttccgga
     2701 tcagaagatt gccgtggtca cgaagctgca gaaggccggg cgcatcgtcg ccatggcggg
     2761 cgacggcgtc aacgacgccc cggcgcttgc cgcagctgat gtcggcatag ccatgggaac
     2821 cggcaccgac gtcgcgatgg agagtgccgg catcacgctc ctgaagggcg acctcggtgg
     2881 catcgttcgc gcgcggcggc tctcgcaggc aaccatgcgc aacatccggc agaacctatt
     2941 cttcgcgttc gtctacaacg ccgccggcat cccgattgcc gccggtatcc tctatcctca
     3001 tttcggcatc ctgctgtcgc cgatcattgc cgccgccgcc atggcgctgt cgtcagtgag
     3061 cgtcgtcggc aacgcattgc gattgcgcgt gacgcctctg tagtcagcat cgcttcaagt
     3121 tgaatcggcg gggatggttg attcacctct ccccgcttgc ggggagaggt tggattgcga
     3181 agcaatccgg gtgaggggga ccaaccgcgc gctctctgcc accgaacttg cggaagcagc
     3241 ccctcacccc aaccctctaa gagcaagctt cgctcgtctc gaccccgcga agagcgggga
     3301 gagggagaag tggtccgccg attcaagtca aagcaatcgc gctgtcgctc aatcgcatcc
     3361 tgacgtcagg cggccgcggc ggaagtcctg ccaggcaatc ctgcgcgggc gagcccgcca
     3421 tagagcggct cgcccggcat ttccgatcgc aggatcgagc gcaccgcgac cagcttgtcg
     3481 atgtcgatgc cggtggcaaa acccttgctc tcgcaaagaa acaccagatc ctcgaacacc
     3541 acattgccgg tcgcgccggg cgcaaacggg cagccgccaa gaccgccgag cgagccgtcg
     3601 agaatgcgcg cgccggcatc gagcgcggcg gacgcgttcg caatccccat gccccttgtg
     3661 tcgtgcaggt gaatgcagac cggtttcgcg ccggacattt tcacgatcgc cctggtcagc
     3721 tctccgacct gcttcgggcc ggcatagccg acggtatcgg cgatggcgac gaaatcgacc
     3781 ccggcctcga ggcatttttc cgccaatttc atcacttccc gcggctctac cggaccggta
     3841 atcgaacagc ccagcgccat cgagatcgcc gcattgacga tcgacttgtg cgggctggcg
     3901 tcgcgcagct cgcaaagccg tttgacattg gcgatggcct gatcgcgcga gcggtttgca
     3961 ttggcctggc tgtgctcttc ggtggccgag accaccgaag caatttcggc cacgcctgag
     4021 gccagcgcct cattgacgcc acgctcgttc agcgccagcg cgatgccgtg cgcgccggga
     4081 agcgccgcca ccgtgcgcac gacttcacgc acatcggcga actgcggaaa cgtgcgcgcg
     4141 ggcaggaacg agccgacctc gaaatgccgc acgccggcgc catattcgtc gcggatccag
     4201 cgctgcttcg ccgcggtcga cggaaacgtt tttacaagct gcagaccgtc tctcagacca
     4261 acctcgcgca acgacacgcg atcgtcggga taaacgtctt gaaggcgggt catgcgagcc
     4321 tcacagcggt tttgccggtt ggagaaaatt cttgtgcctc aagctcggat cgcagggccg
     4381 atgtatcggc gccgagcacc ggcactttga gtccctcgcc gatactcgcg ccgttccact
     4441 cgatcggaag cgcggggacg cggaacgcct cgccattggc attggtgttg ttgacgaggc
     4501 cgccgggacg cagcacatgc ggatcatcga acagatcctc ggggcgattg atcggcgaga
     4561 agcagatgtt gaggcggtcg agcgtcgcgg aaaggtccgc cgagttccag cgtttgatca
     4621 cttccgcaac atgcggaatg atttttgcgc gcgccaggat gcggtcggtc gtggtacgca
     4681 gagccggatc gtccagaaaa tcgtgcaagc cgaactcgcg gcaaaaactc tgccagtggc
     4741 cttcggtgac gacgccgatg aagatgcgct ctccgcccgc ggtctcgaaa atatcgtaga
     4801 tcggccaggc atgttcgcgc tccggcatcg agcgcggttt tatcccggtc atctcatatt
     4861 cgaccatgtg ctgcgccacc aggaaaaggc agttctcaaa cagtccgatg cggatgtcgg
     4921 cgcctgcagc gccgccgcgc ttctggtaga gcgcggcgag aatggcgatc gcgccgaaca
     4981 tgccgcccat gatgtcgttg gccgacgagc cgacgcgctg cggtttgtcc atcgtgccag
     5041 tcatggcggc gagccccgac atcatctgca ccacctcgtc gagcgccggt cgatgctcgt
     5101 agggacccga aagaaaaccc ttgtgcccgg cgacgatcag atgcggatat tttcgacgca
     5161 gttcgtccgc acccaacccc tgcttttcga gctgcccgtc gcggaaattt tccaaaaaca
     5221 cgtcggcgct cgccagcagc cgatccatcg tctcgcagtc ttcgggcctg gtgaaatcaa
     5281 gcaccacgct gcgcttgccg cggttgaaca gcggaaagaa cgaagttccc atgccgccaa
     5341 ggctgcgcgt cttgtcgcct gcgggcggct ccaccttgat gacctcggcg ccgagctgcg
     5401 ccaggatcat gccgcaggtc ggccccatca ccatgtgggt catctcaatg acccggaccc
     5461 ctgataacgg caggccctgc gtgctcatcg gcgtctcccg gttccctcta tatcgctttg
     5521 ttagaggaaa gtaggccttc gagcgctatc tgccaaacat aatagcgcgg cgatcagaag
     5581 cgcgtcagac aacgcagcaa tgcgtccggc gctcgaaaag aaacccgcag gctatcattc
     5641 gttggcgcca cgccttgccc ttgccgaagc ctgcgggcat gtctagctct cgcgaagcta
     5701 tcggagagga taagcgacca tgctagatgc cgccgtcaag gcgctttcgc aaatcctgtc
     5761 gccgccgatg cgttccattc tgtggcgctc gatcgggctg gcgctggtgc tgatcgtggt
     5821 cttggcggtc ggcctgcagc ggctcctgag ttggtttgcg gtgtacggcg aggactgggc
     5881 cgaggccatg ctggggccgg gctttcatac cccgctcaat atcctcgcct ggatcgtctc
     5941 gatcgccgcc gggctcggcg tcgtgttggg cgcggtgttc ttgatgcccg ccatcacctc
     6001 gctggtggca agcgtgttcg tcgacgaggt cgccgatcat gtcgagcgcg aacattatcc
     6061 cgcggaacgg cccggcaccg cgctgccgtt cgggcttgcc atgaccgaag gcatcaagac
     6121 cgcgttgctg accatcctgg tctatctgat cgcgctgccg ttcgtattcg ttgccggtgc
     6181 gggtttcatc gccttcttca tcgccaccgc atggctgttg ggccgggaat atttcgagct
     6241 cgcggcgatg cggtttcggc cgcccgccga agccaaggcg atgcgcaagc agaatgcggc
     6301 aatagtgttc acggccggac ttgtgatcgc ggcgttcgtg tcgatcccga tcgtcaacct
     6361 ggcgacgccg ctgttcggca tggcgttcat ggtccacatg cacaagcggc tctccggccc
     6421 gcggccggaa ttgatcgagc gggagcggaa gacgagcgtg cccgtggggt gatccctcat
     6481 ggtgaggagc gcgtcttcgc gcgtctcgaa ccatgaggcc gatccttcga gacgccgcgc
     6541 aagcgcgcgg ctcctcagga tgaggctcac accgtctttt ccggccagcg gcagagatcg
     6601 ttgatcaggc agacctcgca gcgcggcttg cgcgccaggc aggtgtaacg gccgtgcagg
     6661 atcagccagt gatgggcgtg cagcttgaat tcggcgggaa ccacgcgctc gaggccaagc
     6721 tccacctcga gcggcgtatt gcccggggca agaccggttc gattggcgac acggaatacg
     6781 tgcgtgtcga ccgccaaagt gtgctcgcca taggccatgt tgagcacgac gttggcggtc
     6841 ttgcggccgg cgccgggcag cgattcaagt tcagtgcgag tgcgcggcac ctcgccgccg
     6901 aacccggcga tcagcttttc cgacagcgcg atgacgttct tcgccttgtt gcggtagagc
     6961 ccgatggtct tgatgtagtc gcgcaacctt tcctcgccga gcgccagcat tttctgcggc
     7021 gtatcggcaa ccctgaacag ctctcgcgtc gccttgttga cgccggcgtc ggtcgcctgc
     7081 gccgacagaa cgaccgcgac caaaagtgtg taggggttga gatgttccag ctcacccttc
     7141 ggctcgggat tggctttgcg aaagcggctg aacgcctcat agacctcggc cggcgtccag
     7201 ggcttgattg ccttgagcga tttctttgcc gcgcgcgcct ggcccggatt ggttgcgggt
     7261 ttttttgcgg atttttttcg cgcccgaccg gccgcaggtc gatggcgcgg ttttttgacg
     7321 cgttgtggcg gcgtgggttt cgatttcgtg agcggcttgc gggtgatttt cgccatcatc
     7381 ggggtatact gatacgcgat gaccgcaggc aacgactttg atccgggact ggcgcagacg
     7441 gaattgtttt ccgcgctgct caccccgcac cgttcgctga accgcaccgg ctttctggtg
     7501 ctgatggtgt ttctcagcgc ggtcagtttc gccgcaggcc ttgcgtttct gctgatgggc
     7561 gcctggccgg tgctcggctt tttcggcctc gatgcgcttg cgatctactg ggcgttccgg
     7621 gtcaatttcc tccgcgccag ggccagcgag gaaatctcca tcacgccgtc cgaactgcgg
     7681 gtgcgactgg tcagccaccg tggccatgtg gtcgaatggg tgctcaatcc gctctgggta
     7741 cagctcgacc agaaaaccca tgccgaattc ggcatcgaaa agctttatct ggtctccagg
     7801 ggacgc
//
LOCUS       DATNOI010000304         2062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_185879,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000304 DATNOI010000000
VERSION     DATNOI010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185879"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VN968_11895"
     CDS             <1..598
                     /locus_tag="VN968_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HXL29977.1"
                     /translation="LGAVPGKSLDPRTLLIVGGAGGVGSILIQLARRLTGLTVVATAS
                     RPESQKWCLDLGAHAVIDHAKPMKEQIEKLKGPPVGLIASLTNTDQHYKALAEIIAPQ
                     GKYGLIDDPPEFNVSVFKGKAVSVHWESMFTRSSFQTPDMIAQHFLLNDVADLIDKGV
                     LRTTLDQTFGTINAANLRRAHALLESGKSRGKIVLEGW"
     gene            complement(748..1542)
                     /locus_tag="VN968_11900"
     CDS             complement(748..1542)
                     /locus_tag="VN968_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL29978.1"
                     /translation="MKIDLKGKTALVTGSTTGIGHAVARGLAAAGAEVTINGRAQGKV
                     DTAAAALTKAVPGAKVRGVAADVSTAAGCKTLVAALSEVDILINNAGIFEPKGFFDIP
                     DEDWTRFFEVNVMSGVRLSRAYFPGMLKRNWGRIVLISSESALNIPKEMIHYGVTKTA
                     QLALSRGLAEMTRGTAVTVNSVLPGPTMSEGVETFVKDLARQNGQSVEEAASQFVKQF
                     RPTSLLQRFASVDEIANMVVYISSKQASATNGAALRAEGGIVQTIA"
     gene            complement(1663..>2062)
                     /gene="rplQ"
                     /locus_tag="VN968_11905"
     CDS             complement(1663..>2062)
                     /gene="rplQ"
                     /locus_tag="VN968_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HXL29979.1"
                     /translation="RKLNRTAEHRRAMFANMCAALIKHEQIVTTLPKAKELRPIVEKL
                     VTLGKKGGLAMRRQAISEMRDLEQTKKLFDVLAARYRDRQGGYTRIIKAGFRYGDNAP
                     MAVIEFVDRDVDAKGLDSGPEQEKADAEAA"
BASE COUNT          387 a          661 c          616 g          398 t
ORIGIN      
        1 tctcggcgcg gtgcccggca agagcctcga tccccgcacg ctgttgatcg tcggcggcgc
       61 cggcggcgtc ggctcgatcc tgatccagct cgcgcgccgc cttactggct tgacggtcgt
      121 ggcaaccgcg tcgcggccgg aatcgcaaaa atggtgcctc gatctcgggg cgcacgccgt
      181 catcgatcat gcaaaaccga tgaaggaaca gatcgaaaag ctgaaaggcc cgccggtcgg
      241 gctgatcgca agcctcacca acaccgacca gcattacaag gcactggccg agatcattgc
      301 gccgcagggg aaatatggat tgatcgacga tcccccggaa ttcaatgtca gcgttttcaa
      361 gggcaaggcg gtgtccgttc actgggaatc gatgttcacc cggagctcgt tccagacacc
      421 ggacatgatc gcccagcatt ttctgctcaa cgacgtcgcc gacctcatcg acaagggcgt
      481 gctgcgcacc acgctcgatc agacctttgg caccatcaat gccgccaacc tcaggcgtgc
      541 ccacgcgctg cttgaatccg gcaagtcgcg cggcaagatc gtgctggagg ggtggtaggg
      601 ctgtccccgt ccgtcattgc gagcgaagcg aagcaatcca tcttgatgct tgcggcgcta
      661 tggattgctt cgtcgcttcg cttctcgcaa tgacaggttg gtaaagcagc agggtgggca
      721 aaggcgcagc gacttccgcc ttgcccctca cgcaatggtt tgcacgatgc cgccctcggc
      781 gcgaagcgcg gcgccattgg tggccgaggc ctgcttcgag ctgatataga ccaccatatt
      841 ggcgatctcg tcgacgctgg caaagcgctg cagcagcgag gtcgggcgaa actgcttgac
      901 gaattgcgac gccgcctctt ccaccgactg gccgttttgc ctagcgagat ccttgacgaa
      961 tgtttccacc ccctccgaca tcgtcggccc cggcagcacc gaattgacgg tcaccgcggt
     1021 gccgcgcgtc atctccgcga gcccgcggga cagagcgagt tgcgctgtct tggtgacgcc
     1081 atagtggatc atttccttcg gaatattcag cgcggactcc gacgagatta aaacgatgcg
     1141 gccccaattg cgcttgagca tgcccgggaa ataagcgcgc gagagccgca cgcccgacat
     1201 cacgttgact tcaaaaaaac gggtccagtc ctcatcggga atgtcgaaga accccttggg
     1261 ttcgaagatg ccggcattgt tgatgagaat gtccacttcc gacagcgcgg ccaccagcgt
     1321 cttgcacccg gccgccgtcg agacatcggc ggcaacgccg cggaccttgg cgcccggcac
     1381 ggccttcgtc agcgccgcgg ccgcggtgtc gaccttgcct tgcgccctgc cgttgatggt
     1441 gacttcggcg ccggctgcag caagccccct ggcgacggca tgtccgatgc cggtggtcga
     1501 gcccgtcacc agcgcagtct ttccctttag atcgatcttc atgagatatc tccatccgtg
     1561 atgacggaga tatcgggagc gccgcgcata agcgcaatcg ccggcagtgc ccaataaaaa
     1621 agcggcgcac gcggcgccgc ctttaaaaac caatttaggc agttacgccg cttcagcgtc
     1681 cgccttttcc tgctcgggac ctgaatcgag gcccttggca tcgacatcgc ggtcgacgaa
     1741 ttcgatcacc gccatcggcg cgttatcgcc gtagcgaaag ccggccttga tgatgcgggt
     1801 gtagccgccc tgccgatccc tgtagcgggc ggcgaggaca tcgaacaatt ttttggtctg
     1861 ctcgagatcg cgcatctcgc tgatcgcctg gcggcgcatg gcgagaccac ccttcttgcc
     1921 gagcgtcacc agcttttcca cgatcgggcg cagctccttg gccttcggca gcgtggtgac
     1981 gatctgctcg tgcttgatca gcgccgcgca catattggca aacatcgcgc gccgatgttc
     2041 ggcggtgcga ttgagcttgc ga
//
LOCUS       DATNOI010000305        20100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_306492,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000305 DATNOI010000000
VERSION     DATNOI010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 20100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20100
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306492"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..645)
                     /locus_tag="VN968_11910"
     CDS             complement(193..645)
                     /locus_tag="VN968_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HXL29980.1"
                     /translation="MTVEPYHFEFMAEAIREAEISIAQGGLPIGAVLTRDRKIIARGH
                     NRRVQEQNVILHGEMSCMRNAGLIPFHDTVMYTTLSPCSMCAGAIGLFKLSLLVIGES
                     VTFPGSKDILAQFGIPYIDLKDERSVNMMKSWLSVPANERLWQGDIGN"
     gene            complement(702..1007)
                     /locus_tag="VN968_11915"
     CDS             complement(702..1007)
                     /locus_tag="VN968_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5076 domain-containing protein"
                     /protein_id="HXL29981.1"
                     /translation="MAGPKEQPLPPDVMSRDDATEVLRAFVVDGGLSIAFARAFEEPD
                     MWGLLLVDIARHAARAYARESDYTEEEALTRIVEMFEAEIARPTDVGSATLRSQQGH"
     gene            complement(1011..2417)
                     /locus_tag="VN968_11920"
     CDS             complement(1011..2417)
                     /locus_tag="VN968_11920"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HXL29982.1"
                     /translation="MPIQVLMPALSPTMEKGNLSKWLKKEGEAIKSGDVIAEIETDKA
                     TMEVEATDEGTLGRILIPEGTEDVAVNTPIATILADGESAADLGKASAPAKAEKAAES
                     APPAEAKAKAPAPKDEPAKPAPSAPKAAAEPDPEVPEGTEMVTMTIREALRDAMAEEM
                     RRDPDVFLMGEEVAEYQGAYKVSQGLLQEFGARRVIDTPITEHGFAGIGIGAAMAGLK
                     PIVEFMTFNFAMQAMDQIINSAAKTLYMSGGQMGCSIVFRGPNGAAARVAAQHSQDYS
                     AWYSQIPGLKVVAPFSAADYKGLLKAAIRDPNPVIFLENEMLYGHSGEVPKLDDYVIP
                     IGKARIVRSGGHVTLVSWSNGMSYALKAADELAKEGIEAEVIDLRTLRPMDTDSIVAS
                     VKKTGRAVTVEEGWQQNGVGAEIAARIMEHAFDYLDAPVARVSGKDVPMPYAANLEKL
                     ALPSVAEVVAAAKAVCYR"
     gene            complement(2460..3476)
                     /gene="pdhA"
                     /locus_tag="VN968_11925"
     CDS             complement(2460..3476)
                     /gene="pdhA"
                     /locus_tag="VN968_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HXL29983.1"
                     /translation="MALLKKSAAKESGNGSTSPLEFTRHQELRALRDMLLIRRFEEKA
                     GQLYGMGAIGGFCHLYIGQEAIVVGMQMALKQGDQVITGYRDHGHMLATGMEAKGVMA
                     ELTGRHGGYSKGKGGSMHMFSKEKNFFGGHGIVGAQVSLGTGLAFANRYRGNDLVSLA
                     YFGDGASNQGQVYESFNMAELWKLPVIYVIENNRYAMGTSVTRSSAQTDFSKRGISFN
                     IPGEQVDGMDVRAVKAAGDMAVAWCRQGKGPFILEMQTYRYRGHSMSDPAKYRTREEV
                     DKVRHDQDPIEQVRNRLLAAKMSEQDLKAIDAEVREIVNAAADFAQHDPEPEASELYT
                     DVYR"
     gene            complement(3628..3945)
                     /locus_tag="VN968_11930"
     CDS             complement(3628..3945)
                     /locus_tag="VN968_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HXL29984.1"
                     /translation="MVSRSRLKSFLTGLALYTMAAAMIGYFGVNAYTGKYGLNARQEL
                     DSEIVALTTELARLKRERAEGEQRVSLLRSDRVDPDMLDERARYQLDYANPRDLVRIV
                     NPN"
     gene            complement(4014..4571)
                     /locus_tag="VN968_11935"
     CDS             complement(4014..4571)
                     /locus_tag="VN968_11935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXL29985.1"
                     /translation="MATPYNIVVLVGSLRKESFTLKIAKALSGLAPDTLKLDVVTLHD
                     ISFFNQDLEAAPPADWVAFREKLHNSKGVLFVTPEYNRSIPGVLKNAIDVGSRPYGKS
                     SFLGKPVGIVSNSPGPLGGVSAAKHLQNILPGISGPILGQPEIYLNGVGDAFDDQGEL
                     IKESLQAVMKQYIDAFAAFVEKQNK"
     gene            complement(4797..5123)
                     /locus_tag="VN968_11940"
                     /pseudo
     CDS             complement(4797..5123)
                     /locus_tag="VN968_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685874.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            5429..6064
                     /locus_tag="VN968_11945"
     CDS             5429..6064
                     /locus_tag="VN968_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29986.1"
                     /translation="MTARPTGRTRPHISDPDAAMTAPAVDKITLQVTLTRDDYARYVA
                     IADKRNSSWSNLAWYVVALFAAVPLAFFFESLGRGLAAASADAALIGKFSLFAFLFGG
                     LTMQLSVLILRRLAMRRYVAVTLNPLEPKQVVFDTIGVTMTGGISEYRWRWAAISGLT
                     AQRGLLVLWIGSFAVIVPGRSFDSAAARDAAIAFIRARQSDAASAASTPGT"
     gene            complement(6187..6579)
                     /locus_tag="VN968_11950"
     CDS             complement(6187..6579)
                     /locus_tag="VN968_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HXL29987.1"
                     /translation="MSTDLRYLAFTAILTASLWIPYVVAQVMTNGPLQPRNYVDPTPR
                     PLPLWGKRADRVYVNAVETFAPFAALVIVAHLTGKANAMTAFWAASYFWLRLAHAAVY
                     LLGIPLVRTLLFTLGYVAIVGIFWELIK"
     gene            complement(6679..7818)
                     /locus_tag="VN968_11955"
     CDS             complement(6679..7818)
                     /locus_tag="VN968_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXL29988.1"
                     /translation="MEIDKTGLQLRSLIKNSGELEISLASVPTPEPGPDEVVVRVEAS
                     PINPSDLGLLIGAADMTSAKASGTKDAPVITAKVPEAAMKAMAGRLDESMPVGNEGAG
                     VVVKTGSSDAAKALMGKTVAMIGGAMYAQYRCLKAADVLPLPAGTTPAEGASCFVNPL
                     TALGMTETMRREGHKALVHTAAASNLGQMLNKICLKDGIGLVNIVRSAEQAEILRKIG
                     AKHIVDSTAPTFMDDLTNALVETGATLAFDAIGGGKLAGQILTCMEIAANKTAKVYSR
                     YGSSVHKQVYIYGSLDPRPVELNRAFGMAWGVGGWLLFPFLMKIGQAEGARLRARVVA
                     ELKTTFASHYTQVVSLQEALQLNHIAVYAKRATGEKYLINPNKAG"
     gene            complement(7871..9052)
                     /locus_tag="VN968_11960"
     CDS             complement(7871..9052)
                     /locus_tag="VN968_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HXL29989.1"
                     /translation="MTHPMLFTPLEIRGVRLKNRVVVAPMHQYSAVEGFATDWHLVNA
                     GKYAQGGAGLVIMESTKVARNGCGTVGDTGLWNDGFIPALARCAAFIKAHGAVAGIQL
                     GHSGRKARLTRPWEGGKPLRGDEPEIFDWEGWELVAPSAVPHTEKSPVPRALSHNEVR
                     DLAVKWGEAAGRADTAGFDVLEIHAAHGYLIHQFLSPVANTRTDQYGGSELNRMRFAI
                     EVAECVRAVWPAEKPLFMRLSCEDDAGWGPDESVRLARLLKARGVDVIDCSSGGTLAH
                     SPMDGARARKYGYQVPYAEKIRKEADVMTMAVGHIVHADQAEAILREGRADLTALARE
                     IMYNPNWPMDAAQKLGADPDFDMVPPPYSYWLGKRAKSAFEGRPSTWAAGLEDHTAPK
                     L"
     gene            complement(9120..10403)
                     /gene="eno"
                     /locus_tag="VN968_11965"
     CDS             complement(9120..10403)
                     /gene="eno"
                     /locus_tag="VN968_11965"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXL29990.1"
                     /translation="MTAIVDIIGREILDSRGNPTVEVDVVLEDGSIGCAAVPSGASTG
                     AHEAVELRDGDKARYLGKGVLKAVEAINGEIFETLNDMAVEEQVQIDQLMIGLDGTAN
                     KSRLGANAILGVSLACAKAAAESFDMPLYRYVGGTSARTLPVPMMNIINGGVHADNPI
                     DFQEFMIMPVGAGTFAEALRCGSEIFHTLRGELKKAGHNTNVGDEGGFAPNLPSADAA
                     LEFVMAAIGKAGYSAGSDVMLALDCAATEFFKDGNYVYGGENKTRSRSEQAKYLADLA
                     SRYPIVSIEDGMSEDDMDGWKELTDLVGKKCQLVGDDLFVTNVTRLADGIKNGRANSI
                     LIKVNQIGTLTETLSAVEMAYKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSL
                     ARSDRTAKYNQLLRIEQELGSQAKYAGKAALKALG"
     gene            10539..11015
                     /gene="queF"
                     /locus_tag="VN968_11970"
     CDS             10539..11015
                     /gene="queF"
                     /locus_tag="VN968_11970"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HXL29991.1"
                     /translation="MSKAPGKSSKSQDLQLGRAVEWPRTPEEARLDRVPNPQSDTHYV
                     VRFTVPEFTSICPVTGQPDFAHLVIDYVPGQWLLESKSLKLFVASFRNHGAFHEDCTV
                     MIGKRIAGEIKPKWLRIGGYWYPRGGIPIDVFWQTGKLPVGVWIPDQGVASYRGRG"
     regulatory      11100..11163
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            11246..12460
                     /locus_tag="VN968_11975"
     CDS             11246..12460
                     /locus_tag="VN968_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL29992.1"
                     /translation="MTVVAKPNYALREIPGGIWALGFVSMLMDISSEMIHALLPVYLV
                     TVLGTSMVTVGVIEGVAEATASITKIFSGALSDWLGKRKLLAVLGYGLAAFTKPVFPL
                     APSVSWLIGARFVDRVGKGIRGAPRDALVADIAPTHLRGASFGLRQSLDTVGAFLGPL
                     LGIGLMWWTVDNFRAVFWVAVVPAFLAFALMIFAVREPARPERLRQVHNPISLAEIKR
                     LGASYWWIVGIATVFTLARFSEAFLILRAQSVGLPIMLVPAVLVLMNVVYALAAYPAG
                     VASDRMNRTTLLIVGLVLLIAADLALALLPSIGGVALGVVLWGLHMGLTQGMFATLVA
                     DASPPELRGTAYGFFNLLTGVAMLAASVIAGALWDATGPKATFVAGACLAFLALAGLL
                     GVRGQIRPVVIE"
     gene            12475..12561
                     /locus_tag="VN968_11980"
                     /pseudo
     CDS             12475..12561
                     /locus_tag="VN968_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472930.1"
                     /note="NADPH-dependent; catalyzes the reduction of
                     7-cyano-7-deazaguanine to 7-aminomethyl-7-deazaguanine in
                     queuosine biosynthesis; incomplete; partial in the middle
                     of a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
     gene            12736..13209
                     /locus_tag="VN968_11985"
     CDS             12736..13209
                     /locus_tag="VN968_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29993.1"
                     /translation="MRGWFRQATLVAIASVSLISAALAVDPKTNGSEKPHRLVIQVDQ
                     NDPAVMNLALNNATNVIEYYRAKGEDVDVDVVTYGPGLHMLRADTSPVQDRIKSLKDA
                     AFPSKIQFSACNVTKEGMEKKEGHPISVLAGAVVVPSGVVRLMELQEKGWSYLRP"
     gene            complement(13260..14297)
                     /locus_tag="VN968_11990"
     CDS             complement(13260..14297)
                     /locus_tag="VN968_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL29994.1"
                     /translation="MMRALLIATALSAAGGAVWAQPGNAAPTGMELCFQSARGADSIC
                     SNPANDAVQRLDCLQKARTTLLQCLEHVPQEIATGAASLEKAAGKVTSETPSGTAPPD
                     LPTATVVPDKPPAPVSSDKPAGAASPVTPTAAVSADRPSADMPTAAVSSDKPTAAALP
                     DKPTAAVSPDKPAAAASPDLPARAVDIAPKPRDPNWIISETTSPVDYAPVMTAAIRLP
                     FSVKHAPTTFAIRCRGGRTELLVRTEGTWRASRTREVQVDYQINDQPLVKLTWTSSPD
                     GKTAIYRDDAVGLLQSLPDGARLKISVLDEPGPSHEATFQLTGLDAIREKIAVACKWP
                     PAANKISSEKH"
     gene            complement(14294..15340)
                     /locus_tag="VN968_11995"
     CDS             complement(14294..15340)
                     /locus_tag="VN968_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXL29995.1"
                     /translation="MNGITMTILTRLAGILIVTFALCGSTIAQSHRIHSHRHHGSPSS
                     SDISGEAAIRDRLNASTIGLAGGLLEGAPIHFATEIARVVNDGGAVHVLPIVTRGPTE
                     NVNDLLYLRGVDAAIINGDSLEEYKSQVPQIQQRLTYLLNLFPSELHIFVRPEIRSLA
                     DLAGKKVNFNTQGTAAAYSGPLIFSRLGIDVDKMFIPHPVALEQMKRGEIAGVVFVTS
                     KPVDAFVKGKWEEGFKFLPVEYGPNFEDYYLPSYLEPTDYPNLVKGERIATIAVPTIL
                     ASFNWRPGSPRYLRVARFVDELFSRTDKLQAPGFDPKWKNVNLATRVPGLERFQPAQE
                     WLDRASPAKQSEHP"
     gene            complement(15547..16725)
                     /locus_tag="VN968_12000"
     CDS             complement(15547..16725)
                     /locus_tag="VN968_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL29996.1"
                     /translation="MPPVLNIIALSTFAASLSARALDPVLPHVASDFNVSIAAAAGFA
                     SVFAFTFAIIQPVLGAAADLFGKARLVILCLVLLGLANILGALSSSFSLLFATRILAG
                     IGAGGVFPIALGLTSDLIAPDKRQVAIGRTLAGAMTGNLLGASLSGLIGDFLGWRGVL
                     AVLGSLAVVASVAVAIGFRGGALRRTPAKVDLSVLRHGYRTIFANPNARVCYTAVFIE
                     GCCVLGLFPYVASFLFEQGVTSLSISGLVIAGFAVGGLFYTLSVSRFLPRLGVNGMMM
                     SGAVLMGLQLAIVALGPPWQLQAGNFILMGWGFYMIHGSLQVFASELSVEARATALSL
                     HSFFFFMGQTIGPIAYGFGILNVGKIPTLLASAVVIIALGMVCARLLRQTRPADAGAS
                     "
     gene            complement(16762..17613)
                     /gene="kdsA"
                     /locus_tag="VN968_12005"
     CDS             complement(16762..17613)
                     /gene="kdsA"
                     /locus_tag="VN968_12005"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HXL29997.1"
                     /translation="MNANISAAPVVTIGSVEFGNALPISIIAGPCQLESRAHALEVAG
                     ALKEIATRLKVGLIYKTSFDKANRTSVSAARGIGLAQALPIFAEIRSSLGMPVLTDVH
                     EAGQCAEVAEAVDVLQIPAFLCRQTDLLVAAAATGKVVNVKKGQFLAPWDMANVVAKI
                     TGAGSPNVLVTERGASFGYNTLVSDMRALPILARTTGAPVIFDATHSVQQPGGKGTSS
                     GGEREFVPVLARAAVAVGVAGVFIETHPDPDRAPSDGPNMVPLREFEGLVKTLMAFDA
                     LAKSLAR"
     gene            complement(17662..18537)
                     /locus_tag="VN968_12010"
     CDS             complement(17662..18537)
                     /locus_tag="VN968_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL29998.1"
                     /translation="MPHGLDHIVHAVRDLDAAAEFYARAGFTVGARNRHAWGTHNRIV
                     QLRNCYIEILEVAEPGKIVPHEARSFSFGAFNRDFLDRREGFSMLILNSSNAIEDARA
                     FEAAGIGGYDVFDFAREGTRPDGTPVKLAFSLVFAHDPGSLNPGFAACQHHFPENFWD
                     PAFQIHGNGAKTVPGVAMVADNPSDHHIFLKAFAGVDDLRSSSIGVRARTENGDIEIM
                     EPVAFFDQFSLSTDVDGEGMTLNALRLEVADIAQTEALLRQNGIGSQRHVGRLVVPPH
                     AAHGATLIFETAKEG"
     gene            complement(18570..18896)
                     /locus_tag="VN968_12015"
     CDS             complement(18570..18896)
                     /locus_tag="VN968_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HXL29999.1"
                     /translation="MIYETRVYRCLPGRLPALLKRFETITLKLWEKHGIKQAGFFTTL
                     VGESNQELTYLLAWESLADREKKWAAFQSDPDWIAARAKTEEDGQIVGNIVNQLLVPT
                     SFSSVK"
     gene            complement(19059..>20100)
                     /locus_tag="VN968_12020"
     CDS             complement(19059..>20100)
                     /locus_tag="VN968_12020"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXL30000.1"
                     /translation="RSIGIQPDILLCRTDRPIPKEERRKLGLFCNVRESAVIEARDVD
                     NIYAVPEAYHAAGLDDEVLAAFGIEPRIPPQLQSWHVINERVRNPEGAVTIAIVGKYT
                     GMKDAYKSLIEALSHGGIANKVRVNLDWIESEVFENEDPAPFLEHVNGILVPGGFGQR
                     GAEGKIRAAQFARERDVPYFGICFGMQMAVIEAARNLVGIEEANSTEFGPTPEPLVGL
                     MTEWLRGNELEKRSKAGDLGGTMRLGAYPAVLKRGSRVSEVYGGATEISERHRHRYEV
                     NTAYKDRLEQHGLRFSGVSPDGVLPEIVEYEDHPWFIGVQFHPELKSRPFEPHPLFAS
                     FVQAAVVQSRLV"
BASE COUNT         3698 a         6183 c         6181 g         4038 t
ORIGIN      
        1 ttgaccttgt cgccctcttt cttgagccac ttggcaaggt tgcccttttc catggtcggc
       61 gacagcgcgg gcatcagaat gttgatcggc atgctgacct cacaggatat tcttgctcat
      121 tgtcttccaa ggggaagcca atcaaagacc gttgggcgcc atcggcaccc gatgaaagac
      181 cgaagatccg cttcaattac cgatatcgcc ctgccacagg cgttcgttgg cggggacgga
      241 tagccatgac ttcatcatgt tgaccgatcg ctcatcctta agatcgatat aggggatgcc
      301 gaattgagcg aggatatcct tgcttcccgg aaacgtgacg gactcgccga tcacgagcag
      361 cgaaagcttg aaaagtccga tggcgcccgc gcacatcgaa cagggcgaca gcgtggtata
      421 catgacggtg tcatgaaacg ggatcagccc ggcattgcgc atgcatgaca tctcgccgtg
      481 aaggatgacg ttctgctcct gcacccggcg gttatggccg cgcgcgatga tctttctgtc
      541 gcgcgtcagc accgcgccga tcggcagtcc gccttgcgcg atgctgatct cggcttcgcg
      601 aatggcttca gccatgaatt cgaaatgata gggttcaacg gtcacgggtc acgctttcgt
      661 tcgggaactt gcgataacaa acggaataca ggttggcatt gtcagtgacc ttgttgcgac
      721 cggagcgtgg cgctgccgac atcggtcggg cgagctattt cggcttcgaa catctcgacg
      781 atacgcgtca gcgcttcctc ctcggtgtag tcgctctcgc gcgcgtaggc acgcgcggca
      841 tggcgcgcga tgtcgacgag aagcaaaccc cacatgtccg gctcctcgaa cgcgcgcgcg
      901 aatgcgatgg aaagcccgcc gtcgacgacg aacgcgcgca acacttccgt ggcatcatcg
      961 cggctcatga cgtccggcgg caatggctgc tcctttggac cggccatggc tcacctgtaa
     1021 caaacggctt tggcggcggc gaccacctcg gccaccgacg gcaatgcgag cttttcgagg
     1081 ttcgcggcat atggcatcgg cacgtccttg cccgacaccc gcgccaccgg cgcatcgaga
     1141 taatcgaagg cgtgctccat gatgcgcgcg gcgatttcgg cgccgacgcc gttttgctgc
     1201 cagccttcct cgaccgtgac cgcgcggccg gtcttcttga ccgaggcgac gatgctgtcg
     1261 gtgtccatcg ggcgcagcgt gcgcagatcg atcacctcgg cctcgatgcc ttccttcgcc
     1321 aattcgtcgg cggccttcag cgcgtaggac attccgttcg accatgacac gagggtgacg
     1381 tggccgccgg agcgcacgat ccgcgccttg ccgatcggga tcacataatc gtcgagtttg
     1441 gggacctcgc ccgaatggcc gtacagcatc tcgttttcga ggaagatcac ggggttggga
     1501 tcgcggatcg cggccttcag cagacccttg tagtcggcgg ccgaaaacgg cgcgaccacc
     1561 ttgaggccgg gaatctgcga ataccaggcc gagtaatcct ggctgtgctg ggcggcgacg
     1621 cgggcggcgg cgccgttcgg cccgcggaat acgatcgagc agcccatctg tccgccggac
     1681 atgtacagcg tcttggccgc ggagttgatg atctgatcca tcgcctgcat ggcgaagttg
     1741 aaggtcatga attcgacgat cggctttagg ccggccatcg cggcaccaat gccgatgccg
     1801 gcaaaaccgt gctcggtgat cggcgtatcg atcacgcgcc tggcgccgaa ttcctgcagc
     1861 aggccctggc tgaccttgta ggcgccctga tattccgcga cctcctcgcc catcaggaac
     1921 acgtcgggat cccgccgcat ctcttcggcc atcgcatccc gcagcgcttc acggatggtc
     1981 atggtcacca tttcggtgcc ttcgggcact tcgggatcgg gttcggcggc ggcttttggg
     2041 gctgacggag caggctttgc gggctcgtcc ttgggtgcag gcgccttcgc cttggcttcg
     2101 gccggcggcg ccgactcggc ggctttttcc gctttcgcgg gcgcgctggc cttgccgaga
     2161 tcggcagcgc tttcgccgtc agccaaaatg gtcgcgatcg gcgtgttgac cgcgacgtct
     2221 tcggtgcctt cggggatcag gatcctgccg agggtaccct catcggtggc ctcgacttcc
     2281 atcgtcgcct tgtcggtttc gatctcggcg atgacatcgc ccgacttgat cgcctcgccc
     2341 tctttcttca accattttga aaggttgccc ttttccatgg tcggtgacag cgcaggcatc
     2401 agcacttgaa ttggcatggt ggctccagag aatagctctt taggctgcag gctgcgcgct
     2461 tagcgataga catcggtata gagttcggac gcctccggct cggggtcgtg ctgcgcgaag
     2521 tcggcagcgg cgttgacgat ctcccgcacc tcggcgtcga tggccttcaa atcctgttcg
     2581 ctcatcttgg ccgccagcaa acgattgcgg acctgctcga tcgggtcctg gtcatgccgc
     2641 accttgtcga cttcctcgcg ggtgcgatat ttcgccgggt ccgacatcga atggccgcgg
     2701 tagcggtagg tctgcatttc caggataaac ggtcccttgc cctggcggca ccacgcgacc
     2761 gccatgtcgc ccgcagcctt taccgcgcgg acgtccatgc cgtcgacctg ctcgcccgga
     2821 atgttgaagg aaatgccgcg cttggaaaag tcggtctggg ccgacgatcg ggtcaccgat
     2881 gtgcccatgg cgtagcggtt gttctcgatg acgtagatca ccgggagctt ccacagctcc
     2941 gccatattga aactttcgta gacctggccc tggttcgacg cgccgtcgcc gaaataggcc
     3001 aggctgacaa gatcattacc gcgatagcga ttggcgaaag caaggccggt gccgagtgaa
     3061 acctgggcgc cgacaatgcc atggccgccg aaaaaatttt tctccttgct gaacatgtgc
     3121 atggagccgc ccttgccctt ggaatagccg ccatggcgcc cggtcagttc ggccatcacg
     3181 cccttggcct ccattccggt agccagcatg tggccgtggt cacggtagcc ggttatgacc
     3241 tgatcgccct gcttgagggc catctgcatg ccgacaacga tggcttcctg accgatataa
     3301 agatggcaaa agccgccgat cgcgcccatc ccatagagct ggccggcttt ttcctcgaaa
     3361 cgccggatca acagcatgtc gcgcagtgcc ctcagttcct gatgcctggt gaattccaag
     3421 gggctggtgg agccgttacc tgattctttg gcggcgcttt tcttaagtaa ggccatggca
     3481 attccggatg agagagtggg tagccctctc tatcccaact caaaccgcca tgaaaggatt
     3541 gcgtgatgcc gcgagaatct ggccatgccg cagtgcagtg cacgcgacac tttcgaaatc
     3601 gatttcgcag gtttttgaaa ttagagatca gttcggattg acgatccgca ccagatcgcg
     3661 cggattggca taatcgagct ggtagcgtgc gcgctcgtcg agcatatccg ggtcgacccg
     3721 gtcggatcgc agcagcgaga cccgctgctc gccctcggcg cgctcccgct tcagccgtgc
     3781 cagttccgtg gtcagtgcaa cgatctcgga atcgagctcc tgccgcgcat tcaacccgta
     3841 tttgccggta taggcgttga cgccgaaata gccgatcatc gccgccgcca tcgtgtagag
     3901 ggcaagcccg gtgaggaagg atttgagtcg gctgcgcgag accatgggcc cgaggatgca
     3961 gggggtccgg ttaatggaat cctaacaacc ggaccgccga cgcttcgccc cgcctatttg
     4021 ttctgctttt cgacaaaggc ggcaaaggcg tcgatatatt gcttcatgac tgcctgcagg
     4081 gattccttga tgagttcgcc ctggtcgtcg aacgcgtcgc cgacgccatt cagatagatt
     4141 tcgggctgcc cgaggatcgg accggaaatg cccggcaaga tattttgcaa gtgtttggct
     4201 gcgctgaccc cacccagtgg ccccggcgaa ttgctgacaa tgccaaccgg cttgccgagg
     4261 aacgagctct tgccgtaggg ccgcgaaccg acgtcgatgg cgttcttcaa caccccggga
     4321 atcgaacggt tatattcggg cgtgacgaac aagacgcctt tcgagttatg aagcttttcg
     4381 cggaacgcca cccaatcggc cggcggcgcg gcttcaaggt cctgattgaa aaatgaaata
     4441 tcatgcaagg tgacgacatc gagcttcagc gtatcgggcg cgaggccgct cagcgccttg
     4501 gcgatcttga gcgtaaaact ctccttgcga aggctgccga caagcacgac gatattgtac
     4561 ggagtggcca tacgatttcc tttgatggtg ggcgggggcc ggaacggctg accctcaacc
     4621 tagcgtcctc tgaaaaagat gcaagtgcat ccaactacct ctgtcgcgag gaaatcctgc
     4681 tcggcggcgg ttgcacgcgg ggacgcgctt tcagtgtcaa agcccattag cccggacgca
     4741 gatcaggccg cccgataagg acggcctgcc ctgccaaatt ccacggtggc cgatcagatc
     4801 agcttgctgc cggtgttgat gagctgcggg ttgaacggca cggtcaccat gcccttcggc
     4861 atataggcgg cttcggcggc ggccagcgcg tcgtccttcg ggacattggt cacgaacgta
     4921 tccggcatcg gaaacgagcg cgcaccgtcg ttgatcgagg tgcactcgta cgaaccgacc
     4981 ttgtagcgat aaaaaccggg ggcctgtgcg ccagccggcg gcacggcggc ccgcgtgacg
     5041 gagttggcga atggcgtcgc cacagtcgca gcgccgacgg cagctgcgcc ggccaacaga
     5101 tcgcggcgtc tcaaatgaat catgacgtgt cctcccctgg ttcgcactgc gcgatccaca
     5161 gcacttgatt gctcagcggg ggcattatat cggccaagtc cggggccgca atgaaccgct
     5221 tggttcggcg acgacaccaa tccccgaacg caacgggagc tcaccttgat cagcctggcc
     5281 gaccaaaatc tcaccccctc cctcaaccct gacgaaggtc gtcgccccac cgtcgcatct
     5341 ccgcccgggc ctgaacgcgt cactggatcg cgacaccatt ccttcaagcc cgacccgacc
     5401 ctgaacggtg ccaccgcggc aacgacccgt gaccgcgcgg ccgacgggcc gaaccagacc
     5461 tcacatttct gatccggacg ctgccatgac cgcccccgcc gtggataaaa ttaccctcca
     5521 ggtcaccctg acccgcgacg attatgcgcg ctacgtcgca atcgccgaca agcgcaattc
     5581 gagttggagc aatctggctt ggtatgttgt tgcattgttc gcagccgtcc ccctcgcgtt
     5641 ttttttcgag tcgcttgggc ggggcttggc cgccgcttca gccgacgccg cgctgatcgg
     5701 aaagttcagc ctgttcgcgt tcttgttcgg tggtttgacg atgcagctct cagtcctgat
     5761 cttgcgccgg cttgcaatgc gaagatatgt tgcagtgacg ctcaatccgc tcgaaccgaa
     5821 gcaagtcgtg ttcgacacga tcggcgtgac aatgaccggc ggcatttccg agtaccgttg
     5881 gcgttgggct gcgatcagcg gactgaccgc ccaacgcggc ctcctggtgc tgtggatcgg
     5941 aagcttcgcg gtgatcgttc ccggtcggag ctttgacagc gccgccgcgc gcgatgccgc
     6001 gatcgccttc atccgggcgc gacagtccga cgccgcatcg gctgcgtcga cgccgggcac
     6061 gtgaacgcga accgaccttt ccgacagccg cgactggtca gttggccttg ttcgggggga
     6121 tcaggaactt ctggccggcg caaatgtccg gttgtacgcg gagggcaagg ccccctttgg
     6181 cggcaactac ttgatcagct cccagaagat tccgacgatt gccacatagc cgagcgtgaa
     6241 gagcaacgtc ctcaccaggg gaatgccaag caggtacacg gcggcgtgcg cgaggcgcag
     6301 ccagaaatag ctcgccgccc agaacgccgt catcgcgttg gccttgccgg tcaggtgcgc
     6361 cacgatcacc agcgcggcaa aaggcgcgaa ggtctcgacc gcgttgacgt agacccgatc
     6421 cgcgcgtttg ccccataacg gcagcggtcg cggcgtcggg tcgacataat tccgaggctg
     6481 cagcggcccg ttcgtcatca cctgcgcgac cacgtaaggt atccacagcg acgctgtcag
     6541 aattgcggta aaggcgagat atctgaggtc tgtcgacatt tgaaatttcc cccctctttt
     6601 tagaatgtga tttcaacaac tcctgtagct tgatccatca gctatcgatt tgcgcggatt
     6661 tcgctgatcg ccgcaacgtc agccggcctt gttcggattg atcagatatt tttcgccggt
     6721 cgcgcgcttg gcgtagacgg cgatgtggtt cagctgcagc gcctcctgca gcgacaccac
     6781 ctgggtgtaa tggctggcga atgtggtctt gagttcggcg acgacccgcg cgcgcagcct
     6841 ggcgccctcg gcctgtccga ttttcatcag gaacggaaac agcagccagc cgccgacgcc
     6901 ccaggccatt ccgaacgccc ggttcagttc caccggacgc gggtcaaggc tgccatagat
     6961 atagacctgc ttgtgcacgc tcgatccgta gcggctgtag acttttgcgg tcttgttggc
     7021 cgcgatctcc atgcaggtga ggatttgtcc cgccagcttg ccgccgccga tggcatcgaa
     7081 cgccagcgtc gcgccggttt ccaccagcgc attggtcagg tcgtccataa aggttggcgc
     7141 ggtggaatcg acgatatgtt tggcgccgat cttgcgcagg atctcggcct gctcggcgct
     7201 gcggacgata ttgaccaggc cgatgccatc cttgaggcag attttgttga gcatctggcc
     7261 gagattggag gcggctgcgg tgtgcaccag ggctttgtgg ccttcgcgcc gcatggtctc
     7321 ggtcatgccg agcgcggtca gcggattgac gaagcacgac gcgccttcgg cgggcgtggt
     7381 gcccgcaggc agcgggagca catcagccgc tttcaggcag cgatattgcg catacatcgc
     7441 gccgccgatc atcgccaccg tcttgcccat cagtgctttc gccgcatccg atgagccggt
     7501 cttgacgacc acgccggcgc cttcgttgcc gaccggcatc gattcatcga gccgtccagc
     7561 catcgccttc atcgccgctt ccggcacttt cgcggtaatc accggcgcgt cctttgtgcc
     7621 ggacgctttg gcggatgtca tgtcagcagc gccgatcagg agcccgaggt ctgatggatt
     7681 gatcggcgag gcctcgacgc ggaccacgac ttcatcgggg ccgggttcgg gtgtcgggac
     7741 gcttgccagc gatatctcga gttcgccgct gttcttgatc agcgaacgga gctgaagtcc
     7801 ggtcttgtcg atttccaaag ccacggtgaa tctccgttta tttcgcgtct tgaattaaag
     7861 ccgctcgcac tcaaagcttc ggtgcggtgt gatcctcgag tccagccgcc catgtcgatg
     7921 gcctgccctc gaaggctgac ttggcgcgct tgccgagcca ataggaatag ggcggcggca
     7981 ccatgtcgaa gtcgggatcg gcgccgagct tctgtgccgc gtccatcggc cagttgggat
     8041 tgtacatgat ctcgcgggcg agcgcggtca ggtcggcacg gccttccctc aagatcgctt
     8101 ccgcctggtc ggcatgcacg atatgtccca ccgccatggt catgacgtcg gcttccttgc
     8161 ggattttttc ggcataggga acctggtagc cgtatttccg ggcgcgcgcg ccgtccatgg
     8221 gcgaatgggc cagcgtgccc ccggaggaac agtcgatcac atcgacgccc ctcgccttca
     8281 gcagccgcgc cagccggacg ctctcgtcgg ggccccagcc tgcatcgtcc tcgcaggaaa
     8341 gacgcatgaa cagcggcttt tccgccggcc agaccgcgcg cacgcactcg gcgacttcga
     8401 tcgcaaatcg catgcggttg agctcggagc cgccgtattg gtcggttcgc gtattggcga
     8461 ccggcgacag gaactggtgg atcagatagc cgtgggccgc atggatttcg agaacgtcga
     8521 agccggccgt gtcggcgcgc cccgcagcct cgccccactt caccgcgaga tcgcgcacct
     8581 cgttatgcga cagcgcccgg ggcaccggcg atttttcggt gtggggcacc gcactgggag
     8641 ccaccagttc ccagccttcc cagtcaaaga tttcgggctc gtcgccgcga agcggcttgc
     8701 cgccttccca agggcgcgtc agccgcgcct tgcggccgga gtgaccgagc tggatgccgg
     8761 caacggcgcc gtgcgccttg atgaaggcgg cgcatcgcgc cagcgccgga atgaacccat
     8821 cgttccaaag cccggtatct ccaaccgtgc cgcaaccatt gcgcgccacc ttggtcgact
     8881 ccatgatgac gaggcccgcg ccaccctggg catatttacc ggcgttcacg agatgccagt
     8941 cggtcgcgaa accttcgacc gccgaatatt gatgcatcgg cgccacgacg acacgattct
     9001 tgagacgcac gcccctgatc tcgagcggag tgaacagcat tgggtgcgtc attttatttt
     9061 tgattcctcc catcccgcgt ggtccgcatc gatcgcgcgc ggccttcttg tgcgagctgt
     9121 taccccaacg ccttcagcgc cgctttccct gcatatttgg cctggcttcc cagttcctgt
     9181 tcgatccgca gcagctgatt gtatttggcg gtgcggtcgg agcgcgccag cgagccggtc
     9241 ttgatctgtc cgcaattggt ggcgaccgcg agatcggcga tggtggaatc ctcggtctcg
     9301 ccggagcgat gcgacatgac cgcggtgtag cccgccttat acgccatctc gaccgcgctc
     9361 agcgtttccg tcagggtgcc gatctggttg accttgatca ggatggaatt ggcgcggccg
     9421 tttttgatgc cgtcggcgag ccgggtcaca ttggtgacga acagatcgtc gccgaccaac
     9481 tggcacttct tgccgacaag atcggtcagt tccttccagc cgtccatgtc gtcttcggac
     9541 atgccgtctt cgatcgagac gatgggatag cgcgatgcca gatcggcaag atactttgcc
     9601 tgttcggaac gcgagcgggt cttgttctcg ccgccataga cgtaattgcc gtccttgaag
     9661 aattcggtgg cggcacagtc cagcgccagc ataacgtcgg agccggcgct gtaaccggcc
     9721 ttcccgatag cggccatgac gaactcaagc gcggcgtccg ccgacggcag gttcggcgca
     9781 aaaccgccct cgtcgccgac attggtgttg tggccggcct ttttcagttc cccgcgcagg
     9841 gtgtggaaaa tctccgaacc gcagcgcagc gcctccgcga aagtgccggc gccgaccggc
     9901 atgatcatga attcctggaa gtcgatcgga ttgtcggcgt gtaccccgcc gttgatgatg
     9961 ttcatcatcg gcaccggcag cgtccgcgcc gaggtgccgc cgacataacg atagagcggc
    10021 atgtcgaagg attccgccgc ggccttggcg caggcgagcg agacgccgag aatggcgttg
    10081 gcgccgagcc ggctcttgtt cgccgtgccg tcgagaccga tcatgagctg gtcgatctgg
    10141 acctgttcct cgacggccat atcgttcaag gtttcaaaga tctcgccgtt gatggcttcg
    10201 accgccttca gcaccccctt gccgagatag cgtgccttgt cgccatcgcg cagctccacc
    10261 gcctcgtgcg cgccggtgga ggcgcccgaa ggtaccgccg cgcagccgat cgagccgtct
    10321 tccagcacca cgtcgacctc gacggtggga ttgccgcggc tgtcgaggat ttcgcggccg
    10381 atgatgtcga cgatggcggt catgggtgcc tctttggaaa atggctgatt tgatcggttg
    10441 gcgctgcttc tacagcaagg ctgggaagcg gaaaaggccg ccctgcccgt gacggctcgg
    10501 tccggattgt ctaataacgc gcaaaacgga gatcaatgat gtcgaaagcg cccggtaaat
    10561 cgtcgaaatc gcaggatctg cagctcgggc gcgctgtgga atggcccagg acgcccgagg
    10621 aggccaggct cgaccgcgtg cccaatccgc aaagcgacac ccattacgtg gtgcgcttca
    10681 ccgtgccgga attcacctcg atctgtccgg tcaccgggca gccggatttc gcgcatctcg
    10741 tcatcgacta tgtgccgggc caatggctgc tggaatcgaa atccctgaag ctttttgtcg
    10801 cgagctttcg caatcacggc gccttccatg aggactgcac ggtgatgatc ggcaagagga
    10861 tcgcgggtga aatcaaacca aaatggctgc gcatcggcgg ctactggtat ccgcgcggcg
    10921 gcattccgat cgacgtgttc tggcagaccg gcaaattacc tgttggcgtg tggatccccg
    10981 atcagggcgt cgcgtcctat cgcggacggg gatgagggaa ccaccggccg cgtaagcagc
    11041 gcgttcgcgg taacgtgggc ggcagattga ccgctcgcta tattttcgca taagttaaca
    11101 ggagagatgg ggatggagtc ccccgatacc cgctcgtcgc gggctgatga ctcccgctgg
    11161 gtgaggctac caaagcctcg tcgcggtggg agcatgtttt tagcctgggt tttttgttgc
    11221 ctgaacaagt ggattgggca acgcagtgac cgtggtagcc aaaccaaact atgccctgcg
    11281 ggaaattccc ggcggcatct gggcgctcgg cttcgtttcg atgctgatgg acatctcatc
    11341 cgagatgatc catgcgttgt tgccggttta tttggtgaca gtgctaggca cctcgatggt
    11401 caccgtaggc gtcattgaag gcgtcgccga agcaaccgcg tccattacaa aaatcttttc
    11461 gggtgctctc tctgattggc tcggtaaacg caaacttttg gccgtcctcg gttatgggtt
    11521 ggccgccttc accaagccgg tgttcccgct cgctccatcg gtaagctggc tcatcggggc
    11581 gcgcttcgtt gatcgtgtcg gcaaaggcat tcggggcgcg ccgcgcgacg cgctcgttgc
    11641 tgatatcgct cccacacatt tgcgcggcgc aagttttggt ctgcggcaat cgctcgatac
    11701 ggtcggggcc tttctcggcc cgctgctcgg tatcggcctg atgtggtgga cggtcgacaa
    11761 tttcagggcc gtgttctggg tggcggttgt gcccgccttt ctggcgttcg ccctgatgat
    11821 cttcgcggtg cgtgagcccg cgcggccaga gcgattgcgg caggtacata atccaatcag
    11881 ccttgcggag atcaagcgcc tcggtgcgtc ttactggtgg atcgtcggca ttgccaccgt
    11941 cttcacgctg gcacgcttca gtgaagcctt cctgattctg cgcgctcaaa gtgtcggcct
    12001 gccgatcatg ctggtgcccg ccgtcctcgt gctgatgaat gtggtctacg cccttgccgc
    12061 ttatcctgcg ggcgttgcgt ccgatcggat gaaccggact acgttgttga tcgtcgggtt
    12121 agtgctattg atcgcggccg atcttgctct cgcattgctg ccctctatcg gtggggtcgc
    12181 gcttggcgtg gtgctatggg gtttgcacat gggccttacg caagggatgt tcgcgacgct
    12241 ggttgccgat gcgtcgccac cagaactgcg cggaacggcc tacggatttt tcaatctgct
    12301 gacgggagta gccatgcttg cggcgagcgt cattgctggc gccctctggg atgcaacagg
    12361 tccaaaggcg acgttcgtcg ccggagcttg cttggcgttt ctcgcgcttg ccggacttct
    12421 cggtgtgcgt gggcaaataa gaccggtcgt cattgaataa gagcgaaggc gaaggccgcc
    12481 gagatcaaac caaaatggct gcgcatcggc ggctactggt atccgcgcgg cggcattccg
    12541 atcgacgtgt tctggcagac cgcgaattgc cagatggcat gatactcgcg gccaggctga
    12601 cgtcgcgaat tgccatgact tcagcgtgtg aaactgcgga tggtaccatc ttatgtgtcg
    12661 ccgaaattct tgttgacttg ggcgatgttc ctctggttcg tttgaacaag tattttgagt
    12721 ttaaaggagt ttggcatgcg tggatggttt cgtcaggcga cgttggtcgc tattgcttcg
    12781 gtttccctga tctcggcggc tttggcagtt gatccaaaaa cgaatggttc tgaaaaaccc
    12841 catcgtctcg tcattcaagt cgatcagaat gatccagcgg tgatgaatct cgcgctgaat
    12901 aacgcgacca acgtcatcga atattatcgc gcaaagggtg aggacgtcga tgtggatgtc
    12961 gtcacctatg gccccggcct gcacatgctg cgtgccgata cctcaccggt acaggaccgc
    13021 atcaagagtt tgaaggatgc cgcatttccc agcaagatcc agttttccgc gtgcaacgtc
    13081 accaaggaag gcatggagaa aaaagagggc catccgatca gcgtactcgc cggcgccgtc
    13141 gtcgttccgt cgggtgtcgt tcggctgatg gaattgcagg agaagggctg gagctatctc
    13201 cgaccgtgaa ccaacctgcc ggccgctagg ccagtgagtt tgatttcgaa catcccggcc
    13261 tagtgcttct ccgatgagat cttgttggct gctggcggcc acttgcatgc cacggcaatt
    13321 ttttctcgga tggcatccaa tccggtgagt tggaacgtcg cttcgtggct gggacccggc
    13381 tcatcgagca cgctgatttt cagtcgcgcg ccatccggca gcgactgcaa aagcccgacg
    13441 gcgtcgtctc tataaatcgc ggtctttcca tccggggacg acgtccaggt cagtttgacg
    13501 aggggttggt cgttgatttg gtagtcgact tgaacttccc tggtgcgtga tgcgcgccat
    13561 gtcccctcgg tgcgaaccag gagttcggtg cgtcctcccc gacatcgaat ggcgaaagtg
    13621 gtcggcgcat gcttgacgct gaacggcaag cggatcgcag ccgtcatcac cggggcgtaa
    13681 tccaccggag aggtggtctc actgatgatc cagtttggat ctcgtggctt tggcgcaata
    13741 tcaacggccc tcgcaggcag gtccggcgaa gccgcagcag ccggcttgtc tggcgaaacg
    13801 gcggcagtgg gcttgtccgg caaagctgcc gcagtgggct tgtccgacga aaccgcggcc
    13861 gtgggcatgt ctgcagaggg cctgtcagct gaaaccgccg ccgtgggcgt gactggcgaa
    13921 gctgctccag caggcttgtc ggacgaaacc ggtgcggggg gcttgtcagg tacgacggtc
    13981 gcagtgggta agtccggcgg agccgttccg gaaggagttt ctgaagtcac ctttcctgcg
    14041 gctttttcca aggatgcggc tccagtggcg atttcctgcg gaacgtgctc caggcattgg
    14101 agcagcgtcg tgcgtgcctt ctgcaggcaa tccaggcgct gaaccgcatc atttgccggg
    14161 ttggagcaaa tcgagtcggc gcctcgcgcc gattgaaagc ataactccat gcccgtgggc
    14221 gccgcgttcc caggctgggc ccaaacggcc ccgccagcag cggagagagc ggtggcgatc
    14281 agcagggccc gcatcatgga tgctcgcttt gcttggcggg gctcgcgcga tccagccact
    14341 cctgagcggg ttgaaagcgc tccaggccgg ggactcgtgt cgccagattg acgttcttcc
    14401 attttgggtc gaagccgggc gcctgcagtt tgtccgtccg gctgaaaagt tcgtcgacaa
    14461 aacgcgcgac acgaagatag cgcggcgagc cgggacgcca gttgaacgaa gcgagaatcg
    14521 tgggcaccgc aatggtggcg atccgctcgc ctttgacaag gttcggataa tccgtcggct
    14581 caagatatga ggggagatag taatcttcga agttcgggcc gtactcgaca ggcagaaact
    14641 tgaaaccttc ctcccatttg cctttgacga acgcgtcgac cggcttcgaa gtcacaaaga
    14701 cgacgccggc aatctctcca cgcttcattt gctcaagcgc gaccgggtgc gggatgaaca
    14761 tcttgtcgac atcgatgccg aggcggctaa aaatcaaagg accggaatag gcggcggcgg
    14821 tgccctgcgt attgaagttg actttcttgc ccgccagatc cgccagcgat cggatctccg
    14881 gccgaacgaa gatgtgcaat tcggaaggaa acagattcag caaataagta aggcgctgct
    14941 gaatttgcgg cacctgggac ttgtactctt cgagcgagtc accgttgatg atggccgcat
    15001 caactcccct gagatacagc aggtcattga cgttctcggt cgggccgcgg gttacgatgg
    15061 gaagaacgtg cacggcaccg ccgtcattca cgacccgcgc gatttccgtc gcgaaatgga
    15121 tcggcgcgcc ctccaggaga cctccggcca gaccgatcgt cgaggcattg agccgatccc
    15181 ggatcgccgc ctctccggaa atgtccgatg aagacggcga gccatgatgt cgatgcgaat
    15241 gtattcgatg ggattgcgcg atcgttgaac cgcaaagtgc aaaggtgact attagtatgc
    15301 ctgcgagtct tgttaagatt gtcatcgtaa ttccattcac tcaaaaaatt tgcttcgcgc
    15361 ggattatcat accgtcttcg gcgttgatga cctgatcgcg cgcattttcc cttcaattaa
    15421 aggaagatgg tagcactgaa tattatcccg accaaagcaa ggcttggttg ctgtcatgga
    15481 taacggccaa aggcgatgcg ttgcggccta tgttacctcc gcaagccccc ggcctgcgca
    15541 cgcgggctag cttgctcccg cgtcggccgg cctcgtctgc cgcagcagcc gcgcgcagac
    15601 catgccgagc gcgatgatta caaccgcgct cgccagcaag gtcggtatct tgccgacatt
    15661 cagaatgcca aatccgtagg cgatcggacc gatggtctgg cccatgaaga agaagaacga
    15721 gtgcagcgac agcgcggtgg cgcgggcttc caccgagagt tcgctggcaa atacctgcag
    15781 cgatccgtgg atcatgtaga agccccaccc catcaggatg aagttgccgg cctgaagctg
    15841 ccagggcggg ccgagggcga cgatcgcgag ctgcaggccc atcaatacgg caccagacat
    15901 catcatgccg ttgacgccca accgcggcaa aaagcgcgac acgctcaagg tgtagaacag
    15961 tccgccgacg gcgaagccgg cgatcacgag cccgctgatg gaaaggctgg tgacgccctg
    16021 ttcgaacaga aacgacgcga catagggaaa caggccgagc acgcaacagc cttcgatgaa
    16081 taccgcggtg tagcagaccc gcgcattggg attggcaaag atggtgcgat agccgtgcct
    16141 tagcaccgaa agatcgactt tcgccggcgt ccgcctcagt gcgccgccgc gaaagccgat
    16201 ggccaccgcg accgacgcca cgaccgcgag cgagcccagc acggcgagca caccgcgcca
    16261 gccgaggaaa tcgccgatca ggcccgacag cgacgccccg agcagattgc cggtcatcgc
    16321 tcccgccagc gtgcggccga tggcgacctg ccgtttgtcc ggcgcaatca gatcgctggt
    16381 cagcccgagc gcgatcggaa atacgccgcc ggcgccgatc cccgccagta ttcgcgtcgc
    16441 gaacaacagc gaaaacgacg acgacagcgc gccgagaatg ttggcgaggc cgagcagcac
    16501 caggcacaga atcacgaggc gcgccttgcc gaacaaatcc gccgcggcgc ccagcaccgg
    16561 ctggatgatc gcgaaggtga atgcaaacac cgacgcaaac cctgccgccg cggcaatgct
    16621 gacattgaag tcgctggcga cgtgcggcag caccggatcg agcgcccgcg ccgacaggct
    16681 cgcggcgaac gtcgaaagcg caatgatatt caggaccggc ggcatgtgca ggtcatgacg
    16741 gccgcaagcc tgctcgcaac atcaacgcgc caggctcttg gcgagcgcgt cgaacgccat
    16801 cagcgtcttc accaaacctt cgaactcgcg cagcggcacc atgttggggc cgtccgacgg
    16861 cgcgcggtcg ggatcgggat gggtttcgat gaacacgccg gcgaccccga ccgcgaccgc
    16921 ggcgcgcgcc agcaccggca cgaattcccg ctcgccgccc gatgaagtgc ccttgccgcc
    16981 gggctgctgc accgaatggg tggcgtcgaa gatgacgggc gcgccggtcg tccgcgccag
    17041 gatcggcagc gcgcgcatat ccgacaccag cgtgttgtag ccgaacgatg cgccgcgctc
    17101 ggtgaccagc acgtttggac tgccggcgcc ggtgatcttg gcgacgacat tcgccatgtc
    17161 ccacggcgcc aggaactgcc ccttcttgac attgacgacc ttgccggtcg cagccgccgc
    17221 taccagcaga tcggtctgcc ggcacaggaa cgccgggatc tgcagcacgt cgacggcctc
    17281 tgccacctcg gcacattgcc cggcctcatg aacgtcggtg agcacgggca tgccgagcga
    17341 ggagcggatc tcggcaaaga tcggcagcgc ctgcgcaagt ccgatgccgc gcgcggcgga
    17401 gacgctggtg cggttggcct tgtcaaacga cgtcttgtag atcagcccga ccttcaggcg
    17461 cgtcgcgatc tcctttagcg cgccggcgac ttcgagcgca tgcgcccggc tttcgagctg
    17521 acatggcccc gcaatgatcg aaatcggcaa cgcattgccg aattcgaccg atccgatggt
    17581 gacgaccggc gcagccgaga tgttggcgtt caaggcacta tccttttcca ggcgcgacca
    17641 tgccgagcag cgcccggagg atcaaccctc tttagcggtc tcgaatatca gggttgcgcc
    17701 atgggcggca tgcggcggca ccaccagacg tcccacatgc cgctgcgagc caattccgtt
    17761 ctgccgaagc aatgcctcgg tctgcgcgat atccgcgacc tctaggcgca gcgcgttgag
    17821 cgtcatcccc tcgccgtcca cgtcggttga aaggctgaac tggtcgaaaa acgcgaccgg
    17881 ttccatgatc tcgatatcgc cgttctcggt tcgcgccctg acgccgatcg agctcgaacg
    17941 caaatcgtcc acgccggcaa acgccttcag gaagatgtga tgatcgctcg gattgtcggc
    18001 gaccatcgcc acgccaggca cggtcttggc gccattgccg tggatctgaa atgccgggtc
    18061 ccaaaaattt tccgggaaat gatgctggca cgcggcaaag cctggattaa gcgagcccgg
    18121 atcatgcgcg aaaaccagcg agaacgcgag cttcaccggc gttccatcgg gccttgttcc
    18181 ttcgcgggcg aaatcgaaca cgtcatagcc gccgatgccg gcagcctcga acgcgcgtgc
    18241 atcctcgatg gcattgctgg agttgagtat cagcatcgag aaaccctcac gcctgtccag
    18301 aaagtcgcga ttgaatgcgc cgaatgaaaa cgagcgcgcc tcgtgcggca cgatcttgcc
    18361 gggttcggcg acttcaagga tttcgatata acaattcctt aattgcacaa tgcggttgtg
    18421 ggtgccccac gcatggcggt tgcgcgcgcc gacggtaaag ccggcgcgcg cataaaattc
    18481 cgccgccgcg tcgagatcgc ggacggcgtg cacgatatgg tcgagaccgt ggggcatcgg
    18541 gaggcgacct caccccttcg aaaggccggt cacttcaccg acgagaacga ggtcggcacc
    18601 agcaattgat tgacgatgtt gccgacgatc tggccgtctt cctcggtctt ggcgcgcgcc
    18661 gcgatccagt cggggtcact ctggaatgcc gcccatttct tctcgcgatc ggcaagcgat
    18721 tcccacgcca gcagataggt cagttcctga ttggattcgc cgaccagcgt ggtgaagaac
    18781 ccggcctgct tgatgccgtg cttctcccac agttttagcg tgatggtctc gaagcgcttc
    18841 aagagcgccg gcagccggcc cggcagacag cgatagacgc gggtctcgta gatcatggat
    18901 ttcctccgtt ttcttgcgga ggcgttatag ctgttggagg gaaagctgtc ttggctcaat
    18961 caagcacgcc cggcggcgtg gggcgcatga ctgattactc atcccgaggc tgccgcgtcc
    19021 cgaaggataa gccggccggt tcgagacggg ggcttgctct aaaccagtcg gctctgcacc
    19081 accgcggcct gcacgaacga cgcgaacagc ggatgcggtt cgaacgggcg cgatttcagt
    19141 tcgggatgga attgcacccc gatgaaccac gggtggtctt cgtattcgac gatctccggc
    19201 agcacgccat cgggcgaaac acctgaaaaa cgcaggccat gctgttcgag ccggtccttg
    19261 taggcggtgt tgacttcata gcgatggcga tgccgctccg aaatctcggt ggcgccgcca
    19321 tacacctccg acacccggct gccgcgcttg agcaccgcag gatatgcccc caggcgcatg
    19381 gtgccgccga gatcgccggc cttcgatcgc ttctccaatt cgttgccgcg gagccattcc
    19441 gtcatcaggc cgaccagcgg ttcgggcgtc gggccgaact cggtggaatt ggcttcctcg
    19501 atgccgacca gattgcgcgc ggcctcgatc accgccatct gcatcccaaa gcagatcccg
    19561 aaatacggca cgtcgcgctc gcgcgcgaac tgcgccgcgc ggatttttcc ttcggcgccg
    19621 cgctgaccaa aaccgccggg caccagaatg ccgttgacgt gttcgagaaa cggcgcggga
    19681 tcctcgttct cgaacacttc gctctcgatc cagtcgagat tgaccctgac cttgttggcg
    19741 atgccgccgt gcgacagggc ctcgatcagc gatttgtagg cgtccttcat gccggtgtat
    19801 ttgccgacga tggcgatggt caccgccccc tcgggattac gcacccgctc gttgatgacg
    19861 tgccagctct gcaactgcgg cggaatcctg ggttcgatgc cgaacgccgc cagcacctcg
    19921 tcgtcgaggc cggcggcgtg atacgcctcg ggcacggcgt agatgttgtc gacgtcgcgc
    19981 gcctcgatga cagcactttc gcgcacgttg cagaacaggc cgagcttgcg ccgctcttcc
    20041 ttcgggatcg gccggtcggt gcggcacagc aggatgtccg gctggatgcc gatcgagcgc
//
LOCUS       DATNOI010000306         3235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_900288,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000306 DATNOI010000000
VERSION     DATNOI010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900288"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /gene="trpA"
                     /locus_tag="VN968_12025"
     CDS             complement(<1..582)
                     /gene="trpA"
                     /locus_tag="VN968_12025"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HXL30001.1"
                     /translation="MTTRIDARFAELKQEGRSAFITFMTAGDPDPATSLDIIKALPKA
                     GADIIEIGMPFTDPMADGPAIQAAGLRALKAGMTLKKTLAMVRAFRQDDATTPLVLMG
                     YYNPIYIYGVDQFLLDAKSAGVDGLIIVDLPPEEDVELCLPAMQAGLNFIRLATPTTD
                     DKRLPAVLANTSGFVYYVSITGITGSASADTAVV"
     gene            complement(791..2005)
                     /gene="trpB"
                     /locus_tag="VN968_12030"
     CDS             complement(791..2005)
                     /gene="trpB"
                     /locus_tag="VN968_12030"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HXL30002.1"
                     /translation="MNPNLLNSFRSGPDERGHFGIFGGRFVAETLMPLILDLEQAYAA
                     AKADPAFSAEMNGYLKHYVGRPSPLYLAERLTEHLGGAKIYFKREELNHTGSHKVNNV
                     LGQIMVARRMGKKRIIAETGAGQHGVATATLCARFGLECVVYMGAVDVERQQANVIRM
                     EMLGAKVIPVQSGSRTLKDAMNDALRDWVTNVHNTFYCIGTVAGPHPYPMMVRDFQSV
                     IGVETRAQMQEAEGRLPDSLIACIGGGSNAMGLFHPFLDDPGIEIFGVEAAGHGLTQL
                     HAASIAGGRPGVLHGNRTYLLMDDDGQIQDAHSISAGLDYPGIGPEHSWLHETGRVKY
                     LSATDDEALAAFQLLSRLEGIIPALEPAHAIAKVMELAPKRPKDHLMVVNLSGRGDKD
                     IPQVADILRGKK"
     gene            complement(2002..2661)
                     /locus_tag="VN968_12035"
     CDS             complement(2002..2661)
                     /locus_tag="VN968_12035"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HXL30003.1"
                     /translation="MSLIVKICGLSTRETLDVALDAGADMVGFVFFPPSPRHLSLEIG
                     RELGRQARGRAVKVALTVDADDATLANIVEALAPDILQLHGKETVARLRDIKQRFGLP
                     VMKAIAVETAADLAALPGYAAVADRILFDARAPREATRPGGLGAVFDWHLLEKLDFKL
                     PFMVSGGLNAGNVAEAVRVARAGGVDISSGVERAAGVKDPEMIRSFIRAARATEELIV
                     R"
     gene            complement(2727..3120)
                     /locus_tag="VN968_12040"
                     /pseudo
     CDS             complement(2727..3120)
                     /locus_tag="VN968_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490921.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
BASE COUNT          587 a         1054 c         1041 g          553 t
ORIGIN      
        1 gaccacggcg gtatcggcgc tggcgctgcc ggtgatgccg gtgatggaga cgtaatagac
       61 aaagcccgag gtgttcgcga gcaccgcggg cagccgcttg tcgtcggtgg tcggggtcgc
      121 cagccggatg aaattgagcc cagcctgcat cgccggcagg cacaattcaa cgtcttcctc
      181 gggcggcagg tcgacgatga tcaatccatc gacgcccgcg gatttggcgt cgaggagaaa
      241 ttgatcgaca ccgtagatgt agatcggatt gtaatagccc atcagcacca gcggcgtcgt
      301 ggcgtcgtct tggcgaaacg cgcgcaccat cgccagcgtc ttcttcaggg tcatgccggc
      361 cttcagcgcg cgcaaacccg ccgcctgaat cgccggaccg tccgccatcg gatcggtgaa
      421 gggcatgccg atctcgataa tatcggcgcc tgccttcggc agcgccttga tgatgtcgag
      481 cgaggtcgcg ggatcgggat cgcccgccgt catgaaggta atgaaagccg agcggccttc
      541 ctgcttcagc tcggcgaaac gtgcgtcgat gcgggtggtc atggtttggc gcctgttgtg
      601 ctgcagcccc caccccagcc ctcccccgca agcgggagag ggagcaggct ttgccgggtc
      661 gataatctgt tcgaaacaac gcggcttagt ttgagcgact cgcgcgccgc aatgccggcc
      721 gatcccctct cccgcttgcg ggggagggcc agggtggggg cgctcacgcc agactcgacg
      781 gcagccatca tcacttcttc ccccgcaaga tatccgccac ttgcgggata tccttgtcgc
      841 cgcggccgga gagattgacc accatcagat gatcctttgg ccgcttcggc gcgagctcca
      901 tcaccttggc gatggcatgc gcgggctcca gcgccgggat gatgccttcc agccgcgaca
      961 gcaattggaa cgccgccagc gcttcatcgt cggtcgccga cagatatttc acccggccgg
     1021 tttcgtgcag ccatgaatgt tccggcccga tgccgggata atccagcccg gccgaaatcg
     1081 aatgcgcgtc ctggatctgg ccgtcatcgt ccatcagcag atacgtgcga ttgccgtgca
     1141 gcacgccggg ccggccgccg gcgatcgacg ccgcgtgcaa ctgcgtgagc ccgtggccgg
     1201 cggcctcgac accgaaaatc tcgatgccgg gatcgtcgag gaacggatga aacaatccca
     1261 tggcgttgga gccgccgccg atacaggcga tcagcgagtc cggcaggcgg ccttcggctt
     1321 cctgcatctg cgcgcgggtc tcaacgccga tcaccgactg gaagtcgcgc accatcatcg
     1381 gatagggatg cgggccggcg acggtgccga tgcagtaaaa cgtattgtgc acattggtga
     1441 cccagtcgcg cagcgcgtcg ttcatcgcgt ccttcagggt gcgcgagccg gactgtaccg
     1501 ggatcacctt ggcgcccagc atttccatgc ggatgacatt ggcctgctgc cgctcgacat
     1561 cgaccgcgcc catatagacg acacactcga gcccgaaccg cgcgcacagc gtggcggtgg
     1621 cgacgccgtg ctggccggcg ccggtctcgg cgatgatgcg cttcttgccc atgcgacggg
     1681 cgaccatgat ctggccgagc acgttgttga ccttgtgcga accggtgtgg ttcagctcct
     1741 cgcgcttgaa atagattttg gcgccaccga gatgttcggt gaggcgctcg gcgagataca
     1801 gcggcgacgg ccggccgaca tagtgcttga ggtagccgtt catttccgca ctgaacgccg
     1861 gatcggcctt ggcggcggcg taagcctgct ccagatcgag gatcagcggc atcagcgttt
     1921 ccgcgacaaa gcgtccgccg aaaatgccga aatgcccgcg ctcgtcagga ccgctgcgga
     1981 aggaattgag tagatttgga ttcatcgaac aatcaactct tcggtggcgc gcgcggcgcg
     2041 aataaagctg cggatcatct cgggatcctt gacgccggcc gcccgctcca cgcccgagga
     2101 aatatcgacg ccgccggcgc gggcgacgcg aacggcctcc gcgacattcc cggcattgag
     2161 gccccccgaa accatgaacg gcaatttgaa gtcgagcttc tcaagcagat gccagtcgaa
     2221 caccgcgcca agaccgcccg gacgggtggc ttccctgggt gcgcgggcat cgaacaggat
     2281 gcgatcggcc accgcggcgt agccgggcaa ggctgcgaga tcggccgcgg tctcgaccgc
     2341 gatcgccttc atgaccggca atccgaatct ctgcttgatg tcgcgcagcc gcgccacggt
     2401 ttccttgccg tgcaattgca ggatatccgg cgccagcgcc tcgacgatgt tggccagcgt
     2461 ggcgtcgtcg gcatcgaccg tgagcgcgac ttttaccgcg cggcccctgg cctgcctgcc
     2521 gagctcgcgc ccgatctcaa ggctcagatg gcgcggcgaa ggtggaaaga acacgaatcc
     2581 caccatgtcc gcgccggcat ccagcgcgac gtcgagcgtc tcacgcgtgg acaggccgca
     2641 aattttgacg atcagggaca tgtttcaggg cacgggtcag ggtggcgaca ggaaagcgaa
     2701 aaaagcgctg cgcttgcgcc cgcgttctac aacgtcgcgc cctgcttgtc tcgcccggag
     2761 gcgccatatc cgccgccctg caagggggcg gaaaggcgct gcggatcgta gcgtgagggc
     2821 gtcatcgtgc cggcgcgcaa atcggccagt tgcgccctcg cctgccgcgc atcggcctcg
     2881 tgctggcgcg cggcgcgccg ccaatgccgc tgccggaacc aggtcgccga gccgcgccgc
     2941 cacgcccgcg atcgcgaccg cgatgatgac cacgaacagc ggcagcgtga gccccaccga
     3001 cgggtcggcg gaattgaacg gatccagcga caccgtcacc atatgacgat tggcgaccgc
     3061 gaagacgaca aacagcaagc ccagcggaat caggaccacg gcggtgagaa acttgcgcat
     3121 gaccagctct cgcgttgatg gagatcagaa tacgcgcggt ctaaaacgcc tacgcgccgg
     3181 cgtccgatcc cgcgttttcg cggttcagac gttcgcgcat ttccttgccg gtctt
//
LOCUS       DATNOI010000307         3886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2013953,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000307 DATNOI010000000
VERSION     DATNOI010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013953"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VN968_12045"
     CDS             complement(<1..767)
                     /locus_tag="VN968_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL30004.1"
                     /translation="MSISPISAISDAALQAAPADKARGYWTTVGRRIIRDKVSMTCAC
                     ILIAIFLSALCASWLGLADPYQGSMIRRLRHIGTPNYPLGTDELGRDMLARLIYGGRL
                     SLVVGILPVIFAFVIGTSLGLVAGYVGGKINTAIMRTIDVFYAFPSVLLAIAISGALG
                     AGIVNSIVSLTIVFVPQITRVAESVTTGVRNMDFVEAARASGAGPFTIMRVHMLGNVL
                     GPIFVYATGLISVSMILAAGLSFLGLGTKPPEPEWGL"
     gene            complement(772..1725)
                     /locus_tag="VN968_12050"
     CDS             complement(772..1725)
                     /locus_tag="VN968_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL30005.1"
                     /translation="MLAYVARRIVYVIPIVISVALVCFLLVHITPGDPLVAILPADAS
                     QELAAQLRIAYGFDRPLPIQFGLWLWKAVNGDLGHSIATGRPVLSEVMRAVGNTVTLA
                     IAAAMIGFTLGLLFGLIAGYFRDSWIDKVATSIAIAGVSVPHYWLGMVLVIIFSVQLN
                     WLPAVGAGPGGSGAWGWDWEHIRYLILPAITTSVIPMGIVTRTVRALTGDILSQDFVE
                     ALRAKGLRETHVFRHVVKNAAPTAMAVMGLQLGYMLGGSILIETVFSWPGSGLLLNSA
                     IFQRDLPLLQGTILVLALFFVFLNLMVDIAQAAIDPRIKRS"
     gene            complement(1920..3587)
                     /locus_tag="VN968_12055"
     CDS             complement(1920..3587)
                     /locus_tag="VN968_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30006.1"
                     /translation="MRNEKTGKRRATAAVVVLAIAAAVGLPRIAGAETVLRIGMTAAD
                     IPRTLGQPDQGFEGNRFTGLTMYDALTMWDLSSADKASVVIPALATEWKVDDADKKKW
                     TFKLRPGVTFHDGSPFNADAVVWNVEKVLKQDAPQFDPSQVGVTASRMPTLASARKID
                     DMTVELTTKEPDSFLPINLTNLFVASPAKWQKLFDAAEGADAKAKSQAAWAAFARNAS
                     GTGPWKMSSFTPRERLELAKNENYWDKARIPKVDKMLLLPMPEASARTAALLSGQVDW
                     IEAPSPDAVAEIKQRGFSLYTNEEPHVWPWQFSRVEGSPWNDIRVRKAANLCVDREGL
                     KDGLLAGLMVPATGTFEPGHPWRGKPTFEIKYDKPAAQKLMQEAGYGPNKRLTVKTQT
                     SASGSGQMLPLPMNEYLQQALAECYFDVQLDVVEWNTLFTNWRRGVKDPSANGANATN
                     VTYAAMDPFFALVRFLQSSMAPPVSNNWGYINNPKFDELVTKARQTFDPAARDAALAE
                     LHAASVDDAAFLYVAHDVAPRAMSPKIKGFVQPKSWFVDFSPISMTP"
BASE COUNT          771 a         1205 c         1208 g          702 t
ORIGIN      
        1 atcaaccccc attccggctc cggcggcttg gtgcccaggc ccaggaacga cagacccgcc
       61 gccaaaatca tcgacaccga aatcaatccg gtggcataga caaaaatcgg tcccagcacg
      121 ttgccgagca tgtgcacgcg catgatggtg aaggggccgg cgccggacgc gcgcgccgcc
      181 tcgacgaaat ccatgttgcg caccccggta gtgacgctct cagcgacgcg ggtaatctgc
      241 ggcacgaaaa cgatcgtcag cgacacgatc gagttgacga tgcccgcgcc cagcgcgccc
      301 gaaatcgcga tcgccagcaa gaccgaggga aacgcgtaga acacgtcgat ggtgcgcatg
      361 atcgcggtgt tgatcttgcc gccgacatag ccggcgacga ggccgagcga agttccgatc
      421 acgaaagcga agatcacggg gagaattccg accaccagcg acagtcgccc gccatagatc
      481 aggcgcgcca gcatgtcgcg gccgagttcg tcggtgccga gcggatagtt cggcgttccg
      541 atatggcgga gtctgcggat catcgagccc tgatagggat cggccagccc aagccaggac
      601 gcacataatg cggaaagaaa aatcgcgatc aggatgcagg cgcaggtcat gctgaccttg
      661 tcgcgaatga tgcgtcgtcc gaccgtggtc cagtagccgc gcgccttgtc ggcgggcgcc
      721 gcctgcaggg ccgcgtccga tattgcgctg atcgggctga tactcatcgg ctcagctccg
      781 cttgatgcgc ggatcgatcg cggcctgcgc gatatcgacc atcaggttca ggaacacgaa
      841 gaacagcgcc agcaccagga tggtgccctg cagcagcggc agatcgcgct ggaaaatggc
      901 cgaattgagc aacagtcccg agcccggcca cgagaatacg gtttcgatca ggatcgagcc
      961 gccgagcatg tatcccaatt gcagccccat caccgccatc gccgtcggcg cggcgttctt
     1021 gacgacgtga cggaaaacgt gggtttcgcg caagcccttg gcgcgcaacg cctcgacaaa
     1081 atcctgtgac aggatgtcgc cggtcagcgc gcgcacggtg cgcgtgacaa tccccatcgg
     1141 aataaccgag gtggtgatcg cgggcaggat gagatagcgg atgtgttccc agtcccatcc
     1201 ccatgcgccg gagccgccgg gaccggcgcc gaccgcgggc agccagttga gctgcaccga
     1261 aaagatgatc accagcacca tgccgagcca gtaatgcggc accgagacgc cggcgatcgc
     1321 gatcgaggta gcaaccttgt cgatccagct atcgcggaaa tagccggcga tcagcccgaa
     1381 caacagtcca agggtaaatc ctatcatcgc ggccgcgatc gccaaggtga cggtgttgcc
     1441 aaccgcccgc atgacttccg atagcactgg gcgcccggtc gcgatggaat gaccgagatc
     1501 gccgttgacc gctttccata gccagagacc gaactggatc ggcagcgggc gatcaaagcc
     1561 ataggcgatg cgcaattgag ctgcgagctc ctgcgatgcg tccgccggca ggattgccac
     1621 caggggatcg ccgggcgtga tgtgcaccag cagaaagcac accagcgcca cgctgatgac
     1681 gatcgggata acatagacaa tgcggcgggc aacataggca agcacggaat tactttcttc
     1741 tttccttctc cccttgtggg agaaggtggc gagaatgagc gcagcgaatt cgagccggat
     1801 gaggggtcgg tcaccgcgga gagaaacccc tcacccgcct tcgatgcttc gcatcgtagg
     1861 cgacctctcc cacaggggga gaagtgaagt gaagcgcggc cataacgact gagcgaacgc
     1921 tacggcgtca tggaaatcgg cgagaagtcc acgaaccagc tcttcggctg cacgaagccc
     1981 ttgatcttcg ggctcatcgc gcgcggggcg acgtcatggg cgacgtaaag gaacgccgcg
     2041 tcgtcgacgg aggccgcatg cagttcggcc agcgccgcat cgcgggcggc cgggtcgaag
     2101 gtctggcgcg ccttggtcac cagctcgtcg aatttgggat tgttgatata accccaattg
     2161 ttcgagaccg gcggcgccat cgacgattgc aggaagcgca ccagggcgaa gaacgggtcc
     2221 attgccgcgt aggtcacgtt ggtggcattg gcgccgttgg cggaaggatc cttgacgccg
     2281 cgccgccagt tcgtgaacag cgtattccat tcgacgacgt cgagctggac atcgaaataa
     2341 cattcggcca gcgcctgctg cagatattca ttcatcggca atggcagcat ctggccggag
     2401 cccgacgccg aggtctgggt cttgaccgtc aatctcttgt tcggaccgta tccagcctcc
     2461 tgcatcagct tctgcgccgc gggcttgtca tacttgatct cgaaagtcgg ttttccccgc
     2521 cagggatggc ctggctcgaa ggtgccggtt gccggcacca tcaggccggc gagcaagccg
     2581 tccttgaggc cctcgcgatc gacgcagaga ttggcagcct tgcggacgcg gatgtcgttc
     2641 cagggcgaac cttcgacccg cgaaaactgc cacggccaga catgcggctc ctcattggta
     2701 taaagcgaga agccgcgctg cttgatttcc gcgaccgcgt ccggcgaggg cgcctcgatc
     2761 cagtcgacct ggccggagag caatgccgcc gtgcgggcgc tcgcctcggg catcggcagc
     2821 agcagcatct tgtcgacctt tggaatgcgc gccttgtccc agtaattctc gttcttcgcg
     2881 agttcgagcc gctcgcgcgg cgtaaagctc gacattttcc atgggccggt gcccgaggcg
     2941 ttacgcgcga acgcggccca ggcggcttgc gatttcgcct tggcatccgc gccttccgcg
     3001 gcatcgaaca gcttttgcca tttcgccggg ctcgccacga acaggttggt gaggttgatg
     3061 ggcaggaagc tatccggctc cttggtggtc aattcgaccg tcatgtcgtc aatcttgcga
     3121 gccgaagcca gcgtcggcat tcgcgaggcg gtcacgccga cctggctcgg atcgaattgc
     3181 ggcgcatcct gcttgagcac tttctcgaca ttccagacca cggcatcggc gttgaacggc
     3241 gaaccgtcgt ggaatgtgac gccggggcga agcttgaacg tccacttctt cttgtcggca
     3301 tcatcgactt tccattcggt cgcgagcgcg gggatcacga cgctcgcctt gtctgcggag
     3361 gagaggtccc acatcgtcag cgcatcatac atggtcaggc cggtgaagcg gttgccctca
     3421 aaaccctgat cgggctggcc tagcgtgcgc ggaatatcgg cggcggtcat gccgatgcgc
     3481 aagacggtct cggcgcccgc gatccgcggc agccccaccg ctgcggcaat tgccaacacc
     3541 acaaccgccg ccgttgcccg cctttttcca gttttttcgt tacgcatcgc agcaaatccc
     3601 cttctcagct tcggtgatta tttcttgtgc agcagtatgc aatggctgtg ccaaggcggg
     3661 caaccgctca tccattgtga ttggcggtgc aattccttgt gggtccgcac gaggaagaga
     3721 cgcagatgcc taatctggca tcaaccttgc aggttgagtg gttggctgga agtacgccga
     3781 ctaccttttt ccaatggagc ttagttcatg cgcgcccgaa attcgatgct tcttgctgtc
     3841 acggcgattg ccatcggcgc cgccctgccc gcccctgcgg cgcgag
//
LOCUS       DATNOI010000308         5366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1100970,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000308 DATNOI010000000
VERSION     DATNOI010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100970"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..453)
                     /locus_tag="VN968_12060"
     CDS             complement(40..453)
                     /locus_tag="VN968_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXL30007.1"
                     /translation="MSQALVSPTHKATAGFTLIEALVALAIVAVSVVAIGALMGSAAR
                     GSRQLEQHVALVQAAYNALWLAFPSRSPAASSTQSGESIEHLWSAATEPFAIDVGAPT
                     GDVVWVPQKVMLRVRSSSGATIDLETVRLFRKRTE"
     gene            complement(422..895)
                     /locus_tag="VN968_12065"
     CDS             complement(422..895)
                     /locus_tag="VN968_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXL30008.1"
                     /translation="MRSRTGPEAGFTLLEMVCTLAIVGLLAAVLLPAIPRQTSRARLE
                     AYAIETAALLKDDRNTAIRRRLDVTTQVDAPSRSLRSGASGQTVRVPEDVRFETLLPR
                     NCDNRPVVASISFFASGMSCGGVIVLARLDAGYEIRVNWLTGRIEVVPSAGLANP"
     gene            complement(895..1350)
                     /gene="gspG"
                     /locus_tag="VN968_12070"
     CDS             complement(895..1350)
                     /gene="gspG"
                     /locus_tag="VN968_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HXL30009.1"
                     /translation="MARHAHGGTHDLQRGFTLVEMLVVIAIIGMIMGLIGPRVLNYLS
                     ESRVKAARIQIQSFSSALDLFYLDAGRYPSTAEGLAALVRPTSGVAGWAGPYLKGGGV
                     PNDPWNNPYVYRSPGVHAPFDIVSYGADGQEGGTDLAADITTTTSIGAK"
     gene            complement(1391..2605)
                     /locus_tag="VN968_12075"
     CDS             complement(1391..2605)
                     /locus_tag="VN968_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXL30010.1"
                     /translation="MGNFRYRALTQNGEIVHGTISAATAKEVLHRIEYLRLLPIETVE
                     EKAAGPVSRDALALFGRPARGDVTTFTRDLALLMKAGARLEDSLELLANDADTGRLRP
                     IVNKLRSGILAGESFAEVLGLHPSLFPDMYVALVRVGEVSGTLDHVLEVIGAERVRAE
                     AMRRKLTDAMQYPVFVLLAACCVMLFFFIFVLPQFSAVLRDFGARSDATIHTFLDISD
                     FLRGNATELALSAAAAIAAGLLLFRRAGFRSAVVSQVSRLPGIATVFRFYRTSLFCRN
                     LGILLGSGLTLTATLRILIDIMSVTGSAAAWSAAADRVRRGGKLSEALAAANLLPAMA
                     IRMLRLGEETGQLPVLAGRIAEFYEAKLQRSLDRMVGIVGPAAIIAISTVVGGLIVSV
                     MTALLSVTQLVG"
     gene            complement(2610..4295)
                     /locus_tag="VN968_12080"
     CDS             complement(2610..4295)
                     /locus_tag="VN968_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXL30011.1"
                     /translation="MSSNPPVRFADHLRGANPLPRFDAADAPDAATPENGGTAAKSWE
                     ATDLSASDFADEVARLFHLPRIALPDLLAAQSLAKHFSRRFLRETMVFPFQSAQHRAC
                     LAVADPGETAARRAAELVLGGEIDMVVASFEDIATALNRLAGDDEVQARDGGEDLQPR
                     EDDIESLRDLASGAPVVRAVNDLLELAVELRASDIHVEALRVGLSVRMRVDGLLRPVP
                     APVGVLPQAVISRIKIVASLNIAERRLPQDGAARLRVGRLELDVRVAVMPTQHGESAV
                     IRLLPKDRGLLAIERLGFAAHDDRSLRRLLALPHGMIVICGPTGSGKTTTLATALSIL
                     NEPTRKILTIEDPVEYEIPGINQSQVKPGIGLTFATALRSFVRQDPDVIMVGEVRDSE
                     TAHVAVHASLTGHLVLTTLHTENAAAAVPRLLDLGVEDFLLRSTLRAVIAQRLVRRLC
                     DRCKLKKMLGMADIDADPRYAALGFDIGAEVHEPVGCESCGGAGYRGRLGIFEVLELN
                     HEVRSLIHGKTDAAMIDRVAIQSGMTTMLDDGAAKCLAGATSVAEVLRVTTVR"
     gene            complement(4637..4798)
                     /locus_tag="VN968_12085"
     CDS             complement(4637..4798)
                     /locus_tag="VN968_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30012.1"
                     /translation="MTSRSRGAASDQAAAPVRDRENDLRIRDAVAAKMSLHQIIEGQR
                     LALDWRQTP"
     gene            4915..5106
                     /locus_tag="VN968_12090"
     CDS             4915..5106
                     /locus_tag="VN968_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30013.1"
                     /translation="MIKPDGWKFTIGCLSQGRLTSRHPHAESDFQQRVTLSMKAKRSK
                     TQRLCEIASWDFSGRAAEF"
BASE COUNT         1063 a         1715 c         1679 g          909 t
ORIGIN      
        1 agccggattg agagacatgt cgagcgcgcg ccgtggacat cattcggttc gctttcgaaa
       61 cagccgaacc gtttccaggt cgatggtcgc gccagagctc gatcgaaccc gcagcatgac
      121 tttttgcggc acccagacca cgtcgccggt cggcgcgcca acatcgatcg cgaacggttc
      181 ggtagccgcg ctccagagat gctcgatcga ctcgccggat tgagtgctcg aggccgccgg
      241 cgaacgcgac ggaaaggcca gccacaaggc gttgtaagcc gcctggacca gcgcgacgtg
      301 ctgctccagc tgcctgctcc cgcgcgccgc ggaccccatg agcgcgccga tggcgacaac
      361 cgacacggcc acgatcgcaa gcgcgacgag cgcctcgatg agggtaaagc cggctgtggc
      421 cttatgggtt ggcgagacca gcgcttggga caacttcgat ccttccagtc agccagttga
      481 cgcggatttc ataacccgca tcgagcctgg ccagcacgat gacaccgccg caggacatcc
      541 ccgatgcaaa gaagctgatg gacgcaacca ccggccgatt gtcgcaattg cgcggcagca
      601 gcgtttcaaa ccgcacatcc tcggggacac ggacggtttg cccgctcgca cccgagcgca
      661 gcgaacgcga tggcgcatcg acctgcgtcg tcacgtcgag ccttcgcctg atcgctgtgt
      721 ttcgatcgtc cttcagcagc gccgccgtct cgatcgcgta ggcttcgaga cgggcccgcg
      781 aggtttgcct cgggatagcg ggcagcagca cagccgccag caaaccgacg atcgccaacg
      841 tgcagaccat ctcgagcagg gtgaaaccgg cttcgggacc ggtgcggcta cgcattattt
      901 tgcgccgatc gatgttgtcg tggtgatatc cgcggcaaga tccgtcccgc cctcttggcc
      961 atcggcgccg tacgacacga tatcgaaggg agcatgcact ccgggcgatc gatagacgta
     1021 gggattgttc cacggatcgt tcgggacgcc gccacctttg agatacggtc ccgcccaacc
     1081 cgccacgccg gaggtcggcc gcaccagcgc cgccaatcct tcggcggtgg acggatatcg
     1141 ccccgcgtcg agatagaaca ggtcgagcgc gctgctgaaa ctctggatct ggatcctggc
     1201 ggccttgacc ctggattcgc tcagatagtt cagcacgcgc ggtccgatca gccccatgat
     1261 catcccgatg atggcaataa cgaccagcat ctcaaccagc gtgaacccgc gctgaagatc
     1321 gtgcgttccg ccatgggcgt gccgcgccat gccgctgatc cgttcctgtt tcatctcatt
     1381 ccacctgctg ctagccgacg agttgggtaa ccgagaggag cgcggtcatc accgaaacga
     1441 tgaggccccc gacaacggta ctgatggcga tgatcgcggc tggtccgacg atgccgacca
     1501 tgcgatcgag gctgcgctgc agtttggcct cgtagaattc agcgatacga ccggcaagga
     1561 ccggcaactg cccggtctcc tcaccgagcc ggagcatgcg aatcgccatc gcaggaagaa
     1621 ggttcgcggc ggcgagcgct tccgacagct tgccgccgcg gcggacgcgg tcggccgccg
     1681 cgctccaggc cgccgcgctg cctgtcaccg acatgatgtc gatcaggatg cgcagcgtcg
     1741 cggtcagggt caggccgctg ccgagcagga tccccagatt acggcaaaac aagctggtgc
     1801 ggtaaaatcg gaacacggtc gcgatgccgg gcagtcggga gacctgcgac accacggcgg
     1861 aacgaaaccc ggcgcgccgg aacagcagca agccagcggc gatcgcggcg gctgcggaca
     1921 gcgccagttc ggtggcgttg ccgcgcagaa agtcggagat gtcgagaaac gtatggatgg
     1981 tggcgtcgga cctggcgccg aaatcgcgca acacggccga gaattgcggc agcacgaata
     2041 tgaaaaagaa cagcatcacg cagcaggcgg cgagcagcac gaaaacaggg tactgcatcg
     2101 catcggtgag cttgcgacgc atggcttccg ctcgcacccg ctcggcgccg atcacctcaa
     2161 ggacgtgatc gagggtcccg gagacttcgc cgacccggac cagggcgaca tacatgtctg
     2221 gaaacagcga aggatgaagt ccaagcacct cggcgaaact ttcgccggcg aggatgccgg
     2281 aacgcagctt gttgacgatc ggacgcagcc gcccggtgtc ggcgtcgttg gccagaagtt
     2341 cgagcgaatc ttccaacctg gcgcccgcct tcatcagcag cgccagatcg cgggtgaagg
     2401 ttgtcacatc gccgcgggcc gggcgaccga acagagcgag ggcgtcgcgc gagaccggac
     2461 ccgcggcctt ctcctcgacg gtctcgatcg gcaatagccg caggtactcg atgcgatgaa
     2521 gcacttcctt cgccgtcgca gccgagatcg tcccgtgcac gatttcaccg ttctgggtca
     2581 gcgcgcgata gcgaaaattg cccacgggct caccgtaccg tcgtgacgcg aaggacttcg
     2641 gcgactgacg tggctccggc caggcatttc gccgcgccgt cgtccagcat cgtggtcatg
     2701 ccggactgaa tggcgacccg gtcgatcatc gctgcgtcgg tcttgccatg gatgagcgaa
     2761 cgcacctcgt ggttcaattc caggacttcg aagattccga gccggcctcg atagccggcc
     2821 ccgccgcagc tttcgcaccc gacgggctcg tggacctcgg cgccaatgtc gaagcccagc
     2881 gcggcatagc gcggatcggc gtcgatgtcg gccatgccca gcattttttt caacttgcag
     2941 cggtcgcaaa gccgacgcac caggcgctgg gcgatgaccg cccggagcgt cgatcgcaac
     3001 aggaaatcct cgacgccgag atcgagcagc cgcggcacgg ctgccgcggc gttttcggtg
     3061 tgcaatgtgg tcagcacgag gtggccggtc aaagaggcat gaacggcgac atgggcggtc
     3121 tcgctgtcgc gcacctcgcc caccatgatg acatccggat cctgccgcac gaaggatcgc
     3181 aaggccgtgg cgaacgtcag cccgatcccg ggcttgacct gcgactggtt gatgccggga
     3241 atctcatatt caaccggatc ctcgatggtg aggatctttc gcgtcggctc gttgagaatc
     3301 gaaagcgccg tggcgagggt tgttgtcttg ccgctgccgg tcggcccgca gatcacgatc
     3361 atgccatggg gcaacgccag cagacgacgc agcgatcgat cgtcgtgcgc agcgaagccc
     3421 agcctctcga tggcaagcag cccccggtcc ttcggaagca gccggatcac cgccgactcg
     3481 ccatgctggg tcggcatcac ggcgacgcgg acatccagtt ccaggcgacc gacgcgtagc
     3541 cgggcggcgc cgtcctgcgg caatcgccgt tcggcgatat tcaagctggc gacaatcttg
     3601 atccgcgaaa tgacggcctg cggcaagacg ccgaccggcg ccggcactgg cctcagcagg
     3661 ccgtccacac gcatccgaac cgaaagcccg acgcgcaggg cctcgacatg aatgtcgctg
     3721 gcgcgcagct cgacggccaa ttcgagaaga tcattgacgg ctcgcaccac cggcgccccg
     3781 ctggcaaggt cgcgcaggct ttcgatgtcg tcctcgcgag gctgcagatc ttcgccgcca
     3841 tcgcgcgcct ggacctcgtc gtcgccggca agcctgttca gcgcggtggc aatgtcctcg
     3901 aacgacgcca ccaccatgtc gatctcgccg ccgagcacca gttcggcggc ccgcctggcc
     3961 gcggtctcgc cgggatcggc gaccgcaagg cacgccctgt gttgggcgga ttgaaacggg
     4021 aaaaccatcg tctcccgaag aaagcgtctg gaaaagtgct ttgccaggga ctgagccgcc
     4081 agcaagtcgg gcagtgcaat ccgcggcaaa tgaaacagcc gagcgacttc gtcggcgaag
     4141 tcactggccg acaaatcggt cgcctcccat gatttcgccg ccgtcccgcc gttctccggc
     4201 gttgcagcgt cgggcgcatc agcagcatcg aaccgcggca atggattggc gccgcgcaaa
     4261 tggtcggcaa atcgaactgg aggattcgag ctcatggcgc ttcccgtaat ggatcgtttc
     4321 agctcacctc attatcggtg gatcagtcgt gcgaagattt tcgttctcgg tgtcaaatat
     4381 gacgcttgtg agacttgcaa gattagaaca gctacagaca tgcgcatcgg gtgttgcgaa
     4441 cgcgagcaga cgcgcaacgc aggcgttggt gcggcggtcg caagcctgac ccggcatcat
     4501 cgaccgttgc tccatcgcag gcgccggcag agcaacatgg aaaaagacgc atgccgccgc
     4561 agcggcgctt cgcaaacgcg atcgatggct tggtgcgaag tgcgctggtc agagcgggag
     4621 gcggcgcggc ttgacctcag ggcgtttgcc gccaatccag cgccagccgc tggccctcga
     4681 tgatctgatg gagcgacatt ttggctgcga ccgcgtcgcg aattctcagg tcgttctcgc
     4741 ggtcgcgaac cggcgcggcg gcctgatcac tggcggcgcc tcttgaccgg gatgtcaaac
     4801 ctcgatcccc ccgatctcat gtgactatcc cgccagtcca gaactgttgc caaagcgacg
     4861 aatgcgagca gaaccagcga caggaccacc gcctgagaaa tcgttacctg ttccatgatc
     4921 aagccggacg gctggaagtt caccatcgga tgcctctctc aggggcgatt gacctcccga
     4981 caccctcacg ccgaatccga ttttcagcag cgggtcacgc tgtcaatgaa ggccaagcgc
     5041 tcgaaaacgc agcggctttg tgaaatagca tcatgggatt ttagcgggcg cgccgcggaa
     5101 ttctgatgtc attatcgagg caacgaattc catcagcaac gccacaatca ttgcaaggat
     5161 cgttcgggcg cccgatccga ttcattcctc accgcctccg atcaaccatg acgctgccac
     5221 gacgggctag ctactttctg gcgcaaaaat gaagtgtgcg gttgttgcgg gagaatcgct
     5281 cagggcacgg aaaactgcaa ggtcggacgg atttgaaatt gacaggggcg actgtggtct
     5341 gattcattct tgtgaatgat tcgaga
//
LOCUS       DATNOI010000309         2695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2332368,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000309 DATNOI010000000
VERSION     DATNOI010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2332368"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VN968_12095"
     CDS             <1..207
                     /locus_tag="VN968_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXL30014.1"
                     /translation="NFANLHVIMAIRNTEFFEVLLPDAMQKYGLVEDIAIDRAGLVHA
                     PTEPGLGARIDFELIERKKVGVLS"
     gene            210..398
                     /locus_tag="VN968_12100"
     CDS             210..398
                     /locus_tag="VN968_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30015.1"
                     /translation="MSHREAALAACHFLIDWLKTEAPFWKSEETPAGERWVEARAEDD
                     TARERWNPRPEANPHAAR"
     gene            complement(1033..2403)
                     /locus_tag="VN968_12105"
     CDS             complement(1033..2403)
                     /locus_tag="VN968_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30016.1"
                     /translation="MAILTRRSVLRSSLGLAAAGALARPHITNAAATTAELWWVQGFA
                     QEEDVAFKKLIADYEKASGNSIDYSIIPFAPMRQKAISAITSGVVPDILELADFQFLP
                     LNAWADKLVDVSDIVEPIKSQYFDTALRGTYTYNNVTKRRAYYAVPMKCSAVPFNIWQ
                     SLIEKAGYKLEDIPNTWDAFLDFFMPVQDKLRAQGMRNIYAYGYQLTANGVDPAILFQ
                     GFLMAYGGKNLVTPDGKLHTDDPQVREAAIKAITKLTTPFKKGYVPPGVVNWNDADDN
                     NAFHAKLVVMDFDGFSTEVAVYKNKALYDDIVTRGMPLGNDGQKIPSPMASFGALIPK
                     GAKNVDVAKEFLKYAVQPKVLNEYLKGGLGRWVVPMPEIAKSDPFWFKEDPHRTAFAT
                     ETLIDPTLPLYEAFNPAMAEVNAEHVFSVAMLDAMNNGMTPEQAIDKAFKRAEAIFAK
                     YPITQA"
     gene            complement(2497..>2695)
                     /locus_tag="VN968_12110"
     CDS             complement(2497..>2695)
                     /locus_tag="VN968_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30017.1"
                     /translation="FIMSISILVAQIGTRRFVDHLRNDRFALGDLAAAPIDRHDDRLV
                     QRVGQQRRQALGAATAAAINI"
BASE COUNT          517 a          835 c          824 g          519 t
ORIGIN      
        1 aatttcgcca atctgcacgt catcatggcg atccggaaca ccgaattctt cgaggtgctg
       61 ctgcccgacg cgatgcagaa atacggcctc gtcgaggaca tcgccatcga ccgcgccggc
      121 ctcgtccacg cccccaccga gccgggcctc ggcgccagaa tcgatttcga gctgatcgag
      181 cgcaaaaagg tcggggtgct gagctgagca tgagccaccg cgaggccgcc cttgccgcct
      241 gccatttcct gatcgactgg ctgaagaccg aagcgccgtt ctggaagagc gaggagaccc
      301 cggccggcga acgctgggtc gaggcccgcg ccgaggacga cacggcgcgg gagcgctgga
      361 accccaggcc ggaggcgaat ccgcacgccg ccaggtagcg cggccgctcc ctgagagaac
      421 gaggacgggg cgtgggtgtg aggccgccgc ccggcggggg gtttcccctg ccggcgcgga
      481 aaatccgcca aaagcgttgc ggcgcggagt gccgatcggt ccccgtcagg gccggtaagt
      541 ggcttcttat caacgtgttg cggtatcgtg cggtgatgtt ttgggtgccg gacgacagcc
      601 gaaatgaccg gggaatttgc gcgccgcgac agccgaaaaa agcgcctcga ccagccaccg
      661 acagcggaat ttttaaaatt gcgattttgt ccgcgtattg atccgacccg gggtcgatga
      721 ccaaccgaag cggtcagccg agacctggcg cggcgacctc tcctcggccg ccgggcgcca
      781 tcgccgcaac agctccagca ggacgaccgc ggctcggcgc cccgcgacaa tcgaattcca
      841 catgctcatc cgacaattct cttaccaaca accctgtatg ttgctaatat agcacgtata
      901 tcatgttatc gcaagcaaat gttgccggat gagttacctc ttgcccgcga tccgcgggca
      961 cgcgccgacc tcctcgccaa cgccatcaaa gggggagggc tctgaggaga gacagcaccg
     1021 acagctaggc tttcatgctt gggtgatcgg gtatttcgca aagatcgcct cggcccgctt
     1081 gaacgccttg tcgatcgcct gctccggcgt cattccgttg ttcatggcgt cgagcatcgc
     1141 gacgctgaac acgtgctcgg cattgacttc ggccatcgcc gggttgaacg cctcgtagag
     1201 cggcaacgtc ggatcgatca gcgtctcggt cgcgaaggcg gtgcgatgcg ggtcctcttt
     1261 gaaccagaac gggtcgctct tggcgatttc gggcatcggc accacccatc ggccgagccc
     1321 gcccttcaga tactcgttga gcacctttgg ctgcaccgcg tatttcaaaa actccttggc
     1381 gacatcgacg tttttcgccc ccttggggat cagcgcgcca aagctcgcca ttgggctcgg
     1441 gattttctgg ccgtcgttgc ccaggggcat gccgcgggtc acgatgtcat cgtatagcgc
     1501 tttatttttg tagacggcga cctcggtcga aaacccgtcg aaatccatca caacgagctt
     1561 ggcgtgaaat gcattattgt cgtcggcgtc gttccagttg acgacccccg gcggcacgta
     1621 accctttttg aacggggtgg tcagcttggt gatggctttg atcgcggcct cgcgcacctg
     1681 cgggtcgtcg gtgtgcaatt tgccgtccgg cgtgacgagg ttcttgccgc cataggccat
     1741 caggaaccct tgaaagagga tggcggggtc gaccccgttg gcggtcagct gataaccgta
     1801 ggcgtagatg ttgcgcattc cctgtgcccg caacttgtcc tgcaccggca tgaaaaagtc
     1861 gagaaaggca tcccaggtat tcggaatgtc ctcaagcttg tacccggcct tctcgatcag
     1921 cgactgccag atattgaacg gaaccgcgct gcacttcatc ggcaccgcat aataggcacg
     1981 ccgcttagta acgttattgt aggtgtaggt gccgcggagc gcggtatcga aatattgcga
     2041 ttttatcggt tcgacgatgt cgctgacatc gacgagcttg tcggcccagg cgttcagcgg
     2101 caaaaactga aagtcggcca gctccaagat atccgggacg acaccgctgg tgatcgccga
     2161 aatcgccttc tggcgcattg gcgcgaacgg gatgatgctg tagtcgatcg agttgccgct
     2221 cgccttctca taatcggcga tcagcttctt gaaggcgaca tcctcctcct gcgcgaagcc
     2281 ctgcacccac cacagctcgg cggtcgtcgc cgccgcgttg gtgatatggg gtcgcgccag
     2341 cgccccggcg gcagccagcc cgagtgaact gcgaagcact gaacgccgcg tcagaatggc
     2401 cattgtcgtt ccctcccgtt cacgcccgcg aaccgataac ggcccgcgtt tgggctgtag
     2461 cctctcatcc caatgtagaa cggacgctga ggggcctcaa atgttaatcg ctgccgcggt
     2521 cgcagcgcca agagcctgcc ggcgctgctg gccgacgcgc tgaacgagac ggtcgtcgtg
     2581 acgatcgatg ggcgccgccg ccaggtcacc aagcgcgaag cgatcgttac gcagatggtc
     2641 gacaaatcgg cgagtgccaa tctgcgcgac caagatgctg atcgacatga tgaaa
//
LOCUS       DATNOI010000310        13473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_934891,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000310 DATNOI010000000
VERSION     DATNOI010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13473
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934891"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(382..1140)
                     /locus_tag="VN968_12115"
     CDS             complement(382..1140)
                     /locus_tag="VN968_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HXL30018.1"
                     /translation="MAAGTLSPPKVVSREEWLSARRALFVEEKEITHKLDELRKKRRH
                     LPWMKTEKPYVFEGPDGKCTLADLFHARGQLAVYHFMLTPGSDHVCKGCSFVADHFDA
                     ARKHFDDADLSFAVISRAPIQRIEQVKRALGWTFPWVSSYGSDFNFDFGVSFKREDLV
                     AGTAKFNYDTIPLKSSEDMMGASIFAKNETGEIFHTYSAYSRGIENLMGAFMWLDLTP
                     KGRHQFDDGHSRLSDDGRDVRTIGRPTGAETGCH"
     gene            complement(1474..2478)
                     /locus_tag="VN968_12120"
     CDS             complement(1474..2478)
                     /locus_tag="VN968_12120"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXL30019.1"
                     /translation="MMRPTIALAMGDPAGISPELTARLLALDEVAAKARLVVIGDRRV
                     LDDGARIAAVRVDLKNVSPDADFSKEPNGAVFVDLGHLDPSTVEPSTATAEGGRFALA
                     NYRHALTLARDGRAEAVCFTPFNKKAMRLAHPAYDDEISFSAGVVGLDGPASEFNVLG
                     GLWNARVTSHISLAEVAARITTEAVLRALRLTDSSMRGAGFERPRIAVAGLNPHAGDG
                     GNFGREEIDAIEPAVEQARADGIATEGPFPADTVFLRAKNGAFDAVLTMYHDQGQIAM
                     KLMGFDRGVTLLGGFDFPICTPAHGTAYDIAGKGIAGIGATRAAILLAAEMAQKQRRV
                     "
     gene            complement(2498..4003)
                     /locus_tag="VN968_12125"
     CDS             complement(2498..4003)
                     /locus_tag="VN968_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016557348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXL30020.1"
                     /translation="MENLETLMHGFGIALSGNHIFLMLIGVLLGILVGVLPGLGAPNG
                     VSLLLPLTFGMQPVSAIILLSCMYWGALFGGSVTSILFNIPGEPSSVATTFDGYPMAR
                     DGRPTTALATAFGSAAFGALAGVVLITLSASWVAKVALAFGPPEYFAVYFLAFASFVG
                     MGGYPPIKTLVSLAIGFALAAVGIDTVSGSVRLTMGIDELVKGVSFVVAVMGLFGIGE
                     LLVAVEDEFHVKAISSKVEWREVFRTLIGLPRYGWVLLRSAAIGCWMGITPGGPTAAS
                     FMSYGIAKRLSSRRANFGKGEPEGIIAPETADHAAGTSALLPMLSLGIPGSATAAVMM
                     GGLMIWGLNPGPMLFIEQKDFVWGLIASMYLGNIVAVILVLLTVPIFAALMRVPFFVI
                     APLIVIICTVGAYSVSNSYLDVVMMMGFGVLGYLFKKLHYPLAPLVLAIVIGDKAEDA
                     FRQSMLMSRGSLGIFFGNALVTTLVLVGAALLVLPPVWQAIGNMTARRRAN"
     gene            complement(4014..4481)
                     /locus_tag="VN968_12130"
     CDS             complement(4014..4481)
                     /locus_tag="VN968_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXL30021.1"
                     /translation="MVSGRSLEAGTALLTGAFGVAVVVSSLDNGIGWSAAGVDAGTFP
                     FVVGLIILSGSVFNLAQGWRHAREIVLRPSELKRLGMLFIPAAVYVGVIPLIGIYLAS
                     AIYVFGALALHKRGAFLFSGLAAIATAVALYLIFELTFQISLPRGLLGNALGF"
     gene            complement(4492..5496)
                     /locus_tag="VN968_12135"
     CDS             complement(4492..5496)
                     /locus_tag="VN968_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL30022.1"
                     /translation="MSHKPKLAAIVFGLVMGVSGAANAAWQPVKPIEFIATAGPGGGT
                     DNFARAVQAIITKHKLVEQPIVVTNKGGGSGAEGYTYTKAMAGDPYKIVFGTSNAWTQ
                     PMVSKVAYNYTDLTPIAAMVQDEFLLWVKQDAPYQNVTDYLKAVAAKPSGDFKMGGAQ
                     SKDTDETLTRMIDKAAKVKFTYIPFKSGAEAAVQLAGGHIDSHVNNPSESIGQWKGST
                     QRPLCAFSPQRLPDGPKVTATQNWHDIPICVESGLDIPQFQQPRTVWLPGKVTPDQAA
                     FYIDLMKKVRAMPEWKEYIERTSQTDTFLTGEAFGKFIKDDIERTRKVAAEENWLISN
                     "
     gene            complement(5554..6774)
                     /locus_tag="VN968_12140"
     CDS             complement(5554..6774)
                     /locus_tag="VN968_12140"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXL30023.1"
                     /translation="MGPLTGMKVVDLTHVMAGPTCTLMLADMGAEVIKIEKIPAGDDT
                     RYMVPPRIGDEAASFLMMNRNKKGIALDLKTPGGAKVLRRLIGSADLLVENFGPGVMD
                     RLGFGYADLKKEHPALIYCSLSGFGRTGPYKHRRGFDLVAQAMSGIMSFTGERPDGPP
                     VKCGAPLSDITAGILAATGILAAYSHRLKTGEGQWVETSLFEAALVQTYWQAAIGLAT
                     GVAPKAMGSAHPLNAPYQAFEASDKWIVVGGANQKHWLRTLEVLGATELAQDPRFATG
                     ADRMAHLKELEALLSAHFHTQPAEYWLSALEEVGVPCGPVNDMLQALADPQTIAREMV
                     VEVEHSSLGRVKTLGLPIKFSQTPGKVRSGAPRYGEHTRAVLSAYGFDADEIAALREE
                     GAIAAADFAEDEVG"
     gene            complement(6809..7600)
                     /locus_tag="VN968_12145"
     CDS             complement(6809..7600)
                     /locus_tag="VN968_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXL30024.1"
                     /translation="MDDTATLEADLLYEVSDGIGRITFNRPQARNALTFNMYRRLAEI
                     CMAAGNDRSLKVLILTGAGDKAFASGTDINQFRAFSSPQDAIDYETRIDQVLGTLEQC
                     QVPIIAAINGVCAGGGAGIAACCDIRIGTRTAKFGFPIARTLGNCLSMSNLGRLSRLI
                     GAARLKEIIFTARLIEADEAAGLGLLNEVVEDRAALERRAGELARLVASHAPLTLRAT
                     KQALLRMQPAVREDEELILMCYQSRDFREGMDAFLNKRQPQWTGE"
     gene            7770..8414
                     /locus_tag="VN968_12150"
     CDS             7770..8414
                     /locus_tag="VN968_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXL30025.1"
                     /translation="MLHDEVVSRLRHILMEGEIAPGARIPERELCASLAISRTPLREA
                     LKVLAAEGLVVLLPNRGSRAAKLTQKDVNELFEVCEALEATAGELACPRITDEQLREI
                     AALQANMVEHYRARDLLSYYRCNRLIHESIVRAADNAVLAGFYESIAARIRRARFITP
                     MSPEHWALAVQEHEGILNALQRRDAGGLAHILRTHLRRKREEVVQAGFAEANDQ"
     gene            8844..9758
                     /locus_tag="VN968_12155"
     CDS             8844..9758
                     /locus_tag="VN968_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL30026.1"
                     /translation="MILFIVRRLWLAALVCLTVLVVSFILTRLSGDLAASIAGPNATA
                     ADVAIITKNYGLDRPLVVQFLEWAGHAAEGDLGRSFLYHAPVWGLIRDRLPITLTLGL
                     TGLAIALLVALPLGILAAVREGSAMDRGIMMVALLGQAMPGFWFGLVMIVLLGLDLQW
                     LPISGVDTWQGYIMPGIVLAFSAIPALMRLTRSGMIDALAADYIRTARAKGLSRAKIV
                     LKHALRNAAMPVVSIAAVQLGFMLGGSVVIESVFALPGVGYLAWESITKNDFPVVQAV
                     VLFLAVIYIALTLLADMMNALLDPRLRG"
     gene            9755..10648
                     /locus_tag="VN968_12160"
     CDS             9755..10648
                     /locus_tag="VN968_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL30027.1"
                     /translation="MSDSVIAPEVIKQQLPARRRLPAFRHLATLLGAVIIGTAVLVAI
                     FAPYLVPHDPFAQDLNQRLIPPVWMEGSQPAHLLGTDQIGRDYLSRLIYGTRISMLIG
                     VLTVITSGLIGITLGIIGGFYGGRTDDVVMFLITCRLSIPLILVALTVVALVGSSLSV
                     VILTLGLLLWDRFAVVARTTTMQVRNLDYIAAAQAAGASRLHLLLREVLPNIANHLVV
                     VATLEMALAILLEAALSFLGLGVPPPLPSWGLMIAEAKEYMFFSPWVIMTPGVALFVL
                     VLGINLLGDGLRDMLGADLRQ"
     gene            10645..11640
                     /locus_tag="VN968_12165"
     CDS             10645..11640
                     /locus_tag="VN968_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30028.1"
                     /translation="MTTFAPDPVLSVANLRVSFGGRTEAVRGVSLAVGRGETHCLVGE
                     SGCGKSVTALSVMNLLPRGIARTADHLRFQGQDLLGMSERQTARLRGDKMAMIFQEPM
                     TSLNPAYTIGSQMSEVFERHRGATRRQAMDRAAELLARVGITAPGMRLGQYPHQLSGG
                     LRQRMMIAMALMCEPELLIADEPTTALDVTVQAQILRLLQKLQQELGLALLLITHDLG
                     VVARMAQRVSVMYAGEVVETGTVAEVFAAPTHPYTRGLLDCIPVPGKTRTGEPLGSIP
                     GLVPRMPPGFVGCGFRDRCTLAMPECAQTVPPRNAGGDHGYLCRLPPDWKRAVAA"
     gene            11637..12593
                     /locus_tag="VN968_12170"
     CDS             11637..12593
                     /locus_tag="VN968_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HXL30029.1"
                     /translation="MTHAIEARNVYREFQQRGGVVGARRPVRAVDGVTFSLPAGDVLG
                     VVGESGCGKSTLARLIMGLLPPTSGEILVDGRSLAGMDRRERARLIQPVFQDPFSSLN
                     PRQRIRDIVRLPLAAQGDLPKARQVDRVMAMLARVGISSEMAERTPGQLSGGQRQRVA
                     IARALVLHPRIVVCDEPTSALDMSVQAQILNLLAELRRELGLTYLFISHNLAVVEYIA
                     TEVAVMYLGRIVEQAPAQELFRRPAHPYTQALLDSVLTPDPALGIPDVGLGDAYPDPT
                     DIPPGCRFHPRCPSALAKCAATMPDNIRKDGRLVECLLAVPA"
     gene            complement(12656..>13473)
                     /locus_tag="VN968_12175"
     CDS             complement(12656..>13473)
                     /locus_tag="VN968_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30030.1"
                     /translation="LLGGKADWIWGYNADQFANIARMPNLEAVRFGTMRVHFMTVDGA
                     GRSGADNPLTKLKVRQAIISAIDRATMAKQLIQGDSQVIDTPCFPTQFGCDVLAAVKY
                     PYDPARAKQLLTEAGYPNGFDTELVSYATPQVGAAVQNYLKAVGINMRIQQLQIGAAI
                     QLAEAGKAPLYSGSWGSNSVNDTSAFMPYWLGGGLNDYARDADVQKLLKQAGASIDPE
                     ERRKAFGEAIHLATERVDFVPLFSDVRYVAFSKQLNFQGFPDDVPRFYLSTWK"
BASE COUNT         2441 a         4152 c         4240 g         2640 t
ORIGIN      
        1 aatttctggt agagcgtcag cggctcgaac gacaggcgca gatcagaaaa gtcctttttc
       61 aaaggcatcg tcggctccat cagccagcgg gctttaggcg tagcacgatt ttcacaataa
      121 tagtgccgta cggtcgtgac gcccgcagag ggtcattcgg gtcggtttgg cgatgtcctt
      181 gtcacttccg gtttagccct gaaagccgtc attcatcgga agggtcggca tgtctcaaag
      241 gtgccaaaaa cggaagtggt ggcatttcca gaacatgtcc gctgttcccc ccaacaacag
      301 acattcgttg aggcgagcgg cacgtctgct ttgtgccagt ttccggaagt tcccctaatg
      361 cagtgacgtc gccagtaact gtcaatgaca gccagtctcc gcgccggtag gtcgcccgat
      421 tgtacggacg tcccggccgt catcgctaag ccgactgtga ccgtcatcga attgatggcg
      481 gcccttgggc gtgagatcga gccacatgaa ggcacccatc aaattttcaa tgccacggga
      541 ataggccgag taggtgtgga agatctcgcc agtctcgttt ttggcaaata tgctggcgcc
      601 catcatatct tcgctgctct tgagcgggat cgtgtcgtaa ttgaactttg ctgtgccagc
      661 gaccaggtcc tcccttttga atgacacccc aaaatcaaag ttgaagtcgc tgccatagga
      721 tgacacccac gggaaggtcc agcccaaagc gcgtttcact tgctcgattc gctggattgg
      781 cgcgcgtgaa atcacggcaa atgacaggtc ggcatcatcg aagtgcttgc gcgccgcgtc
      841 gaaatgatcg gcgacgaatg agcacccttt gcaaacgtga tcggagcccg gcgtcagcat
      901 gaagtgatag acggcgagtt ggccgcgagc atgaaacaga tcggcaagtg tgcacttgcc
      961 atccggtccc tcgaagacgt agggcttctc agttttcatc cacggcaaat ggcggcgctt
     1021 ttttctaagt tcgtcgagct tgtgcgtaat ctccttctct tcgacgaaca gcgccctgcg
     1081 tgcggaaagc cattcctcac gcgagacgac tttcggtggg gagagggtgc ctgctgccat
     1141 attcaagact cctctgattg ggggttatta aacgtaggtc cccgccggcc ggttccttgc
     1201 ttgcctggca accacacgcg atggcgtctc cctggctaag tgctccaaca ccgccggcga
     1261 tggctgctgg acaaagtgca tgtccgaatc gggtcaatcg cgtcactttg acagaggacc
     1321 ggcgacttcc gatcttcccc cgggaacgga catcgtctcg cccggtcggc atgtctcaaa
     1381 cctgccgaaa accgaacttg cgggaatggc ggcacttggc gcaaagcgga cgttcgcagg
     1441 cgacccgcct cagcgaggtg cgatctggct gcttcaaacc cgcctttgtt tctgcgccat
     1501 ttccgccgca agcaggatag ccgcgcgtgt ggctccgatc cctgcgattc cttttccggc
     1561 gatgtcgtag gccgttccgt gtgccggcgt acagatcggg aaatcaaagc cgccgagcag
     1621 ggtcacgccg cgatcgaagc ccatcagctt catcgcgatc tgaccctggt catggtacat
     1681 cgtgagcacc gcgtcaaacg cgccgttttt cgcgcgaagg aaaaccgtat ctgcgggaaa
     1741 cggcccttcg gtcgcgatac cgtcggcgcg ggcctgttcc acggcgggtt caatcgcgtc
     1801 tatttcctcg cgcccgaaat ttccgccatc gccggcgtgc ggattaaggc cggcgaccgc
     1861 tatccgcggc cgctcgaagc cggccccgcg catcgaactg tccgtgagcc ggagcgcacg
     1921 cagcaccgct tccgtggtga tgcgcgccgc gacttccgcc agcgaaatat gggatgtgac
     1981 gcgggcgttc cagagcccgc ccagcacatt gaattcgctc gccggcccgt cgagcccaac
     2041 cacgccggca gaaaagctga tctcgtcatc ataggcggga tgcgcgaggc gcatcgcctt
     2101 cttgttgaag ggcgtgaaac agaccgcttc ggcgcggccg tcgcgcgcga gcgtcagggc
     2161 atgacgataa tttgccaagg caaagcggcc gccttcggcc gtagcggtgc ttggctcgac
     2221 cgttgatgga tcgagatggc cgagatcgac gaaaaccgca ccgttcggct cctttgagaa
     2281 atccgcatcg ggcgagacgt ttttcaggtc aacccggacg gcggcaattc ttgcgccgtc
     2341 gtcaaggacg cggcggtcgc cgatcacgac gagccgggcc ttggctgcga cctcgtcgag
     2401 ggcgagaagc cgcgcggtca gttccgggct gatgccggcc ggatcaccca tggcgagtgc
     2461 gatagtcggt ctcatcatga tttgcacctt tctgcggtca attcgcgcga cgtcgcgccg
     2521 tcatgtttcc gatcgcctgc cagaccggcg gtagcaccag cagggctgcg ccaaccaaca
     2581 ccagcgtcgt caccagcgca ttgccaaaga atattccaag cgagccgcgc gacatcagca
     2641 tggactggcg gaaggcatcc tcggccttgt cgccaatgac gatggcgagt accagcggcg
     2701 ccagcggata atgaagtttc ttgaacagat aacccagcac gccaaatccc atcatcatga
     2761 cgacgtcgag ataggagttg gagaccgaat aggcgccgac cgtgcagatg atgacgatca
     2821 gcggcgcgat gacgaagaac ggcacccgca tcaacgcagc gaagatcggc acggtgagca
     2881 gcaccaggat caccgcgacg atattgccga gatacatcga agcgatcaga ccccagacga
     2941 agtccttttg ttcgatgaac agcatcggcc cgggattgag cccccagatc atcaggccgc
     3001 ccatcatgac ggcggcggtg gccgatccgg gaatgccgag agacagcatc ggcagcagcg
     3061 cgctggtacc ggcggcatgg tctgcggttt ccggcgcgat gatgccttcc ggctcgccct
     3121 tgccgaaatt ggcgcgccgg gatgaaaggc gtttggcgat gccgtagctc atgaaagagg
     3181 ctgcggtcgg cccacccggc gtaataccca tccagcaacc gatggcggcg ctgcgcaaca
     3241 gcacccagcc atatcgcgga agaccaatga gggtgcggaa gacctcgcgc cactcgacct
     3301 tggaagagat cgccttgacg tgaaattcgt cctcgaccgc gaccagcaac tcgccgatgc
     3361 cgaacaggcc catgaccgcg accacgaagc tgacgccctt caccagttca tcgataccca
     3421 tggtgagccg cacgctgccg gagacggtgt cgatcccgac cgcggcgagg gcaaagccga
     3481 tcgcgagcga taccagggtt ttgatcggag ggtagccgcc catgccgacg aagcttgcaa
     3541 aggcaaggaa gtacaccgcg aaatattccg gcggcccgaa cgccagcgcc actttcgcga
     3601 cccaggaggc cgacagcgtg atgagaacga caccggcgag cgcgccgaag gccgccgaac
     3661 cgaacgcggt cgccagcgcc gtagtcggcc ggccgtcgcg cgccatcgga tagccgtcga
     3721 acgtggtcgc aaccgacgaa ggctcgccgg ggatgttgaa caggatggac gtgaccgagc
     3781 caccgaacaa ggcgccccaa tacatgcagg agagcaggat gatcgccgac accggctgca
     3841 tgccgaacgt cagcggcaac agcagcgaga cgccattggg cgcgccgagc ccgggcagca
     3901 cgccgaccag aatgccgagc agtacgccga tcagcatcag aaaaatatga ttgccggaca
     3961 gggcgatgcc aaatccgtgc atcagcgttt cgagattttc caacgaagtc ccctcagaag
     4021 ccgagtgcgt tgccgagcaa gccgcgcggc aacgagatct ggaacgtgag ctcgaatatc
     4081 agatagagcg ccaccgccgt ggcgatcgcg gcgaggcccg agaagaggaa ggcgccgcgc
     4141 ttgtgtaggg ccagcgcgcc gaacacgtag atggccgagg ccagatagat cccgatcagc
     4201 ggaatgacgc cgacatagac ggcggccgga ataaacagca ttcccagccg tttcagttcg
     4261 ctcggccgca ggacgatttc gcgggcgtgc cgccagccct gcgccaggtt gaatacgctg
     4321 ccgctcagga tgatgaggcc gacgacgaac ggaaaagttc cggcatcgac gccggcggcg
     4381 gaccagccga tgccgttgtc gaggctggag acgaccaccg ccacgccgaa cgcgccggtc
     4441 aggagtgccg tgccggcttc caggctgcgt cccgagacca tcattggtgc cctagttgga
     4501 gatcagccaa ttttcttcgg cggcaacctt gcgggtgcgc tcgatgtcat ccttgatgaa
     4561 cttgccgaac gcctcgccgg tcaggaacgt atcggtttgc gacgtccgct cgatatattc
     4621 cttccattcc ggcatagccc gaaccttctt catcaggtcg atatagaacg cggcctgatc
     4681 gggggtgacc tttcccggca gccacaccgt ccgaggctgc tggaattgcg gaatgtcgag
     4741 gccggattcg acgcagatcg ggatgtcgtg ccagttctgc gttgccgtca ccttcggtcc
     4801 atccggcaaa cgctgcgggc tgaacgcgca aagcgggcgc tgggtgctgc ccttccactg
     4861 cccgatgctt tcgctgggat tgttgacgtg ggagtcgata tggccgccgg caagttgcac
     4921 cgcggcctcg gcgccgctct tgaagggaat gtaggtgaac tttaccttcg ccgccttgtc
     4981 gatcatgcgg gtcagggttt cgtcggtatc cttcgactgc gcgccaccca tcttgaaatc
     5041 gcccgacggt tttgcggcga ccgccttcag atagtccgtc acgttctggt agggcgcatc
     5101 ctgcttgacc cacagcagaa actcgtcctg caccatcgcg gcaatcggcg tcaggtcggt
     5161 atagttgtag gcgaccttcg aaaccatcgg ctgggtccag gcattggagg ttccgaacac
     5221 gatcttgtag ggatcgcccg ccatcgcctt ggtgtaggta tagccttcgg caccgctgcc
     5281 gccgcccttg ttggtgacga caatgggctg ctcgaccaat ttgtgcttgg tgatgatcgc
     5341 ctggacggcg cgcgcgaaat tatccgtgcc gccgccgggt cccgcggttg cgatgaattc
     5401 gatcggcttg accggctgcc aggcggcatt ggccgcgcca gacacgccca tgacgagacc
     5461 gaagacgatc gcagcgagtt tcggcttgtg agacatcgag tcctcccatt tgtctttttg
     5521 tcgattttgt tttcttgttt ttggcttttt gcgttagccg acttcatcct ccgcaaagtc
     5581 ggctgcggca atggctcctt cttcgcgaag tgcggcaatt tcgtcggcgt caaacccata
     5641 ggcacttaaa acggcacggg tgtgctcgcc gtagcgtggc gcgcccgatc gcaccttgcc
     5701 gggcgtctgc gaaaacttga tcggcaatcc cagcgttttg acccggccaa gcgatgaatg
     5761 ctcgacctca accaccatct cgcgcgcgat cgtttgcggg tcggcgagcg cctgaagcat
     5821 gtcgttgacc ggcccgcagg ggacaccgac ctcttccagc gcgctcagcc agtattcggc
     5881 aggttgggtg tgaaagtgcg cggacagcag ggcttccaat tccttcaggt gcgccatccg
     5941 atcggcgccg gtggcaaagc gcggatcctg cgccagttcc gtggcgccca gcacttcgag
     6001 cgtgcgcagc cagtgctttt gattggcacc acccaccacg atccacttgt ccgaggcttc
     6061 gaacgcctga tagggcgcgt tgagcggatg ggccgagccc atcgccttcg gcgccacgcc
     6121 ggtggcgagc ccgatggcgg cctgccaata ggtctgcacg agggccgcct cgaacaacga
     6181 ggtttccacc cactgaccct cgccggtttt caggcgatgg ctataggcgg cgaggatgcc
     6241 cgtggcggcg agaatgccgg cggtgatgtc ggacaatggc gcgccgcatt tgaccggcgg
     6301 tccgtcgggc cgctcaccgg taaagctcat aattccgctc atggcctgcg ccaccaaatc
     6361 gaagccgcgg cggtgcttgt aaggaccggt gcggccgaag ccggacagcg agcagtagat
     6421 cagcgcggga tgctcctttt tcaggtcggc gtaaccgaaa ccgagccgat ccatgacgcc
     6481 gggaccgaaa ttctccacca gcaggtcggc cgacccgatc aggcggcgca agaccttggc
     6541 gccgcccggt gtcttcaggt ccagcgcgat gcccttcttg ttgcggttca tcatcaggaa
     6601 cgaggccgcc tcgtcgccga tcctgggagg aaccatgtag cgggtgtcgt cgccggccgg
     6661 gatcttctcg atcttgatca cctcggcgcc catgtctgcc agcatcagcg tgcaggtcgg
     6721 gcctgccatc acatgcgtca gatcgacgac cttcattccg gtcagagggc ccatggtttc
     6781 tcccgtgatg ttgttgtttg cttgccgatt attcaccggt ccattgcggc tggcgcttgt
     6841 ttagaaatgc gtccatgcct tcgcggaaat cgcggctctg gtaacacatc agaatcagct
     6901 cttcgtcttc gcggactgcc ggctgcatgc gaagcagagc ctgcttggtg gcgcgcaacg
     6961 tcagcggcgc atggcttgcg accagccgcg ccagttcacc ggcgcgccgc tcgagcgcgg
     7021 cgcggtcctc gacgacttca ttcaacaggc caaggccggc ggcctcgtcg gcttcgatca
     7081 ggcgtgcggt gaagatgatt tccttcagac gtgccgcccc gatcaatctt gagagccggc
     7141 cgaggttgga catcgacagg cagttgccga gcgtgcgcgc gatcgggaat ccgaatttcg
     7201 cggtcctggt gccgatgcgg atgtcgcagc acgccgcgat gccggcgccg ccgccggcgc
     7261 aaaccccgtt gatcgccgca atgatcggga cctggcattg ctcgagcgtg ccgagcacct
     7321 gatcgatcct tgtttcgtaa tcgatggcgt cctgcgggga actgaaggca cgaaactgat
     7381 tgatgtcggt gcccgacgcg aacgccttgt cgcctgcgcc ggtaaggatc agcaccttga
     7441 gcgaacggtc gttgcccgcg gccatgcaga tctcggcaag ccgccgatac atgttgaatg
     7501 tcagcgcatt tctcgcctgc ggccggttga aggtgatccg gccgatgccg tcgctgacct
     7561 catagagtag gtcggcttca agagtcgcag tgtcatccat gttgcttcag accggttgga
     7621 ggcgggtgat ttccgttgcg ttaaatccaa cggctaccaa ttctgaatgc aaaatctggc
     7681 ttttgcaaca ggaattcgct atgttttgcc ctgcatagca gattttgaat tcaaaattgg
     7741 tgaattgcac gatcacaggg ggagctttca tgctgcatga cgaggtggtt tcccgcctca
     7801 ggcacatttt gatggaaggc gaaattgcgc cgggcgcccg gataccggag cgcgaattgt
     7861 gcgcgtcatt ggcgatctcg cgcacgccgc tgcgcgaagc cctcaaggtc ctggccgccg
     7921 aagggcttgt cgtcttgctg ccgaaccgcg gatcgcgcgc cgccaagctg acacagaagg
     7981 acgtcaacga actgttcgaa gtctgcgaag ccttggaagc gaccgccggc gaattggcct
     8041 gtccgcgcat taccgacgag caactgcgcg aaatcgccgc cctgcaggcc aacatggtcg
     8101 agcattatcg cgcccgcgat ctgctgtcgt attatcgttg caatcgattg atccatgaaa
     8161 gcatcgttcg cgccgccgat aacgcggtgc tcgccggatt ttatgaatcg atcgcggcgc
     8221 ggatccggcg cgcgcgcttc atcactccga tgtctcccga gcattgggcg cttgcggtcc
     8281 aggagcacga aggcattttg aacgcgctgc agcgacgcga cgccggcgga ctcgcccaca
     8341 ttcttcggac gcaccttcgg cgaaaacgcg aggaagtcgt ccaggctggc ttcgccgagg
     8401 cgaatgatca atgaacttca aacgactgcg ttgggttgta tctccgccgg agcacgctac
     8461 ggccgtttcc ggcgcgaagc ggtcgttcac caagacaacg tcgccaataa gtgccaacaa
     8521 gcgacatcac ggcacccttg cctcgtcgac gggcgtggtg aagttggcgt aggtgcaacc
     8581 acgcccgcgc tggcgatttt tccggccaca agcgtctgac aggcattcca ttgccgtgtc
     8641 ccgcctctgg ttgcgaacga gcgtacgcaa tgaacttctt agctcgtaca cagtgagagc
     8701 ggcggtcgtt gctgtaggct ttgggcaaat tggcgagcac gttgcgtgca tatgcagaaa
     8761 acataggcag atgcggatgt cgccattgct agggttctat atcggaactt gcagcgaccc
     8821 gcgcgttatc gtagtcctcg actatgatct tgtttatcgt ccgcagactg tggctggccg
     8881 ccttggtgtg cctaaccgtt ctggtcgtca gcttcatctt gacacgcctt tccggcgacc
     8941 tggccgcgtc gatcgcaggc ccgaacgcca ccgcggcgga tgtcgcgatc attacgaaaa
     9001 attatggcct cgaccggccg ttggtggtgc agtttctcga atgggccggc cacgctgccg
     9061 agggcgatct cggccgatcc tttctctacc atgccccggt ctggggactg atccgcgacc
     9121 gattaccgat caccctgacg cttggcctga ccggccttgc gatcgcgctg ttggtcgccc
     9181 tgccgctggg cattttggcg gcggtgcggg agggctcggc gatggatcgg ggcatcatga
     9241 tggtggcgct gctggggcag gccatgccgg gcttctggtt cggtctggtc atgatcgtcc
     9301 tgctcggcct cgacctgcaa tggctgccga tttcgggcgt ggacacctgg cagggctaca
     9361 tcatgccggg gatcgtgctg gcgttttcgg ccatcccggc cctgatgcgc ctgacccggt
     9421 cgggaatgat cgacgccctg gcggcggatt acattcgcac cgcgcgcgcc aagggattga
     9481 gccgcgcgaa gattgtcctc aagcacgcct tgcgcaatgc ggcgatgccg gtggtctcga
     9541 tcgcggcggt gcagcttggc tttatgttgg gaggatccgt ggtgatcgag tcggtattcg
     9601 ccctgccggg tgtcggctat ctggcctggg aatcgatcac caaaaatgat tttccggtgg
     9661 ttcaggccgt cgtgctgttc ctggccgtca tctatatcgc cctgaccctg ctggcggaca
     9721 tgatgaacgc gctgctcgat ccaaggctgc ggggatgagt gacagtgtga ttgcaccaga
     9781 ggtgatcaaa caacagctgc cggcccgccg gcgtttaccc gcctttcgtc acctcgccac
     9841 gctgctcggc gccgtgatca tcgggaccgc cgtgctggtc gcgattttcg caccctatct
     9901 ggtgccgcat gatcctttcg ctcaggatct caaccagcgc ctgatccctc cggtgtggat
     9961 ggagggcagc cagccggcgc atcttttggg caccgatcaa atcgggcggg attacctgtc
    10021 gcgtctgatc tacggtaccc gcatttccat gctgatcggc gtgctgaccg tcatcacctc
    10081 cggcctcatc ggcatcacgc tcggaatcat cggcggcttc tatggcgggc ggaccgacga
    10141 cgtcgtgatg ttcctgatca cctgccggct gtcgatcccg ctgatcctgg tggcattgac
    10201 ggtggtggcg ctggttggca gttcgctcag cgtggtgatt cttacgctcg ggctgttgct
    10261 gtgggaccgc ttcgccgtgg tggcgcgtac caccacgatg caggtgcgca acctcgatta
    10321 catcgccgcc gcccaggctg ccggtgcctc gcgcttgcat cttctgctgc gcgaggtgct
    10381 tcccaatatt gccaatcatt tggtggtggt ggcaacgctg gaaatggcac tggcgatcct
    10441 gcttgaagcg gccttgtcat ttctcggcct gggcgtgccg ccgccgctgc cgagttgggg
    10501 gctgatgatc gccgaggcca aggaatacat gttcttcagt ccatgggtga tcatgacccc
    10561 aggcgttgcg ctgttcgtcc tggtgctcgg aatcaacctg ctgggtgacg ggctgcgcga
    10621 catgctgggc gcggatctgc ggcaatgaca accttcgcac cggacccggt tctcagcgtc
    10681 gccaatctgc gcgtgtcctt cggcgggcga acggaagcgg tgcggggcgt atcactggcg
    10741 gtggggcgcg gcgaaacgca ctgcctggtc ggcgagtccg gctgcggcaa atccgttacc
    10801 gcgctatcgg tgatgaacct gctgccgcgc gggatcgccc gcaccgccga tcacctccgc
    10861 tttcaggggc aggatctgct gggcatgtcc gagcggcaga ccgcacggct gcgcggcgac
    10921 aagatggcga tgatcttcca ggaaccgatg acgagcctga acccggccta tacgatcgga
    10981 tcgcagatgt ccgaggtgtt cgaacgccat cgtggggcca cgcggcggca ggccatggac
    11041 agagcggccg aattgctggc gcgcgtgggc atcaccgcgc ccgggatgcg cctgggccag
    11101 tatccccatc aactctccgg cggcctgcga cagcggatga tgatcgccat ggcgctgatg
    11161 tgcgaaccgg agctgctgat cgccgacgaa cccacaacgg ccctcgatgt gacggtgcag
    11221 gcgcagatcc tgcggctgct gcagaagctg cagcaggagc tggggctggc attgctgttg
    11281 atcacgcatg atctcggtgt ggtggcgcgc atggcgcagc gtgtctcggt gatgtatgcg
    11341 ggcgaggtgg tcgaaaccgg cacggtggcc gaggtgttcg ccgcgcccac gcacccctac
    11401 acgcgcgggc tgctggactg cattcccgtg cccggcaaga cgcggactgg cgagcccctt
    11461 ggcagcattc cgggcttggt gccgcgcatg ccgcccggct tcgtaggttg cggatttcgc
    11521 gaccgctgta ctttggccat gccggaatgt gcccagaccg tgccgccgcg caatgccggc
    11581 ggcgatcatg gctatctctg ccggctgccg ccggattgga agcgagctgt ggcggcatga
    11641 cccacgcgat cgaagctcgg aatgtgtacc gggagttcca gcagcgcggc ggcgtggtgg
    11701 gcgcccgtcg cccggtgcgc gcggtggacg gggtgacgtt ctcgcttccg gcgggcgatg
    11761 tgctgggcgt ggtgggcgag tccggttgcg gcaagtcgac gctcgcccgt ctcatcatgg
    11821 gcctgctgcc accaacctcg ggtgagattc tggtggatgg ccgcagcctg gctggcatgg
    11881 accgacgcga acgcgcccga ctgatccagc ccgtgttcca ggacccgttc tcttcgctca
    11941 acccgcgcca gcgcattcgc gacatcgtcc gcctgccgct ggccgcccag ggcgatttgc
    12001 cgaaggcgcg gcaggtcgat cgcgtcatgg cgatgctggc gcgcgttgga atttccagcg
    12061 agatggccga gcgcacgccg ggccagctct ccggcggaca gcggcagcgt gtggcgatcg
    12121 cgcgtgcgct cgtgttgcac ccgcgcattg tggtgtgcga cgagcccacc agtgcgctgg
    12181 acatgtccgt gcaggcgcag attctcaatc tgctggcgga attacggcgg gagcttggac
    12241 tgacctatct cttcatcagc cacaacctgg cggtggtgga atacatcgcc actgaagtgg
    12301 cggtgatgta cctcggccgg atcgtcgagc aggcgccggc gcaggagctg ttccgcagac
    12361 ccgcgcatcc ctacacacag gcgctgctgg actccgtgct gacaccggat ccggcgctgg
    12421 gcattcccga tgtcggtctg ggcgacgcct acccggatcc caccgatatc ccgccgggct
    12481 gtcgtttcca cccgcgttgc ccgagcgcgt tggcgaaatg tgcggcaacg atgcccgaca
    12541 acattcgcaa ggatggccgg ctggtcgagt gtctgctggc ggttccggca taaggcaagc
    12601 gccacgcatc cagatttgat gcggccgcgc cggctgtcga gatcgcgcca gtaacctact
    12661 tccaagtcga caaatagaag cgcggcacat cgtccgggaa gccctggaaa ttcaactgct
    12721 ttgaaaatgc gacatagcgg acgtcgctga acagcggcac gaaatcgacg cgttcggtgg
    12781 cgagatggat cgcctcgccg aaggccttgc ggcgctcttc cggatcgatg ctggctccgg
    12841 cctgtttcaa cagcttttgc acatcggcat cgcgcgcgta gtcgttcaat ccgccgccga
    12901 gccaataggg catgaacgcc gaggtatcgt tgacggaatt gctgccccaa ctccccgaat
    12961 acaacggcgc cttgcccgct tccgccagct ggatggcggc gccgatctgg agctgctgga
    13021 tccgcatgtt gatgccgacg gccttgagat agttctgcac ggcagcgccg acctgcggtg
    13081 tggcatagga cacgagctcg gtatcgaagc cgttgggata accggcctcg gtcagaagct
    13141 gcttggcccg cgctgggtcg tatggatatt tcacggcagc caatacgtca cagccgaact
    13201 gggtggggaa acacggcgtg tcgatgacct gcgaatcgcc ctggatcagc tgcttggcca
    13261 tagtggcgcg gtcgattgcc gagatgatcg cctgccgcac cttcagtttg gtcagcggat
    13321 tgtcggcgcc ggaacggccg gccccgtcga cggtcatgaa atggacccgc atggtgccga
    13381 accgcaccgc ctccagattt ggcatccgcg cgatattcgc gaactggtcg gcattatagc
    13441 cccagatcca atcggctttt ccgcccagta gct
//
LOCUS       DATNOI010000311         5407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_367208,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000311 DATNOI010000000
VERSION     DATNOI010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367208"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VN968_12180"
     CDS             <1..619
                     /locus_tag="VN968_12180"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL30031.1"
                     /translation="RRPLPRRRKNNDKTCAGARVVIRSSRLSLRNWQESDRDTFAALN
                     ATPEVMWDLGGSLDRNESDAKFDRYVAAFDQHGFCRWALEDLDGKFLGSAGVMPSRPG
                     HPLGPHADIGWRLVRPAWGRGYATEAARASLRDVFERVNLKEVLAYTSADNVRSQAVI
                     KRLSMQRAATLDYSEPLGKAVWHGLVWMVRRDDSLDLDHSNDRAR"
     gene            644..1093
                     /locus_tag="VN968_12185"
     CDS             644..1093
                     /locus_tag="VN968_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXL30032.1"
                     /translation="MRARPSARLLVMDSSGRILLFRFVHKNGALAGQDYWATPGGGVK
                     DGETFEQAAIRELKEETGIGVQEIGPEIGRRIFVLQLPNGEHVMADERFFLVRTGDTS
                     LSRDGWTAQEADIMTDHKWWSRDELAQTSETVWPENLATMLDAAMNP"
     gene            1226..2767
                     /locus_tag="VN968_12190"
     CDS             1226..2767
                     /locus_tag="VN968_12190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL30033.1"
                     /translation="MVGFIRPPCDAGIIRGTPAVAAALSQRRKQLTLAATILGSSLAL
                     IDGSVVNIALPAIQQALHADAASTQWIVNAYLLLLGALVLVGGSAADLYGRRRIFLLG
                     IAIFTSASIACGLSPDITVLVVSRAVQGLGAALLMPASLAMLGATFDEHERSHAIGIW
                     AGAGALTAAAGPVLGGWLVDQVSWRAIFLINVPLAIAAAGLAIFFACESRDDQAKPLD
                     WNGAVAVAIGLAAITWGLGAIPSLGFRDKAVLGALGAGIASLLSFVAIEARSRERAMM
                     PLSLYRSRNFSGANMLTLLLYFALGGALYFLPFGLIRLGGYSATQAGAAVLPFALIMG
                     FGASFAGTVADRFGPRLSLTAGPIIAAGGLALLAFADLGKSYWAGVFPALGVFSIGMT
                     VTVPPLTSTVMASVGDAHAGVASGVNNAIARVAGLLAVAALGAVLFASFSRHLAGITP
                     AGANDALNAVLAGQAEVIAGATAAFDRALRTVMLVTAVCAALGGIAGWLWVRPSDPAE
                     PRNFS"
     gene            2850..3143
                     /locus_tag="VN968_12195"
     CDS             2850..3143
                     /locus_tag="VN968_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30034.1"
                     /translation="MHQAANVQFGRAVGEFASWRAVPEDARSPAPAWWWGPAFEVHGA
                     PQPMPAEWCAKLELPEGATYATGAGVFLNALAGQTSLPWPGEFPRKAGHSDVA"
     gene            complement(3259..>5407)
                     /locus_tag="VN968_12200"
     CDS             complement(3259..>5407)
                     /locus_tag="VN968_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXL30035.1"
                     /translation="WHVLAREDGTRSGASFPLLKDGDRAIGVLLFLSLDEGAFTDDLV
                     ELLGRLAENVSFALDNFDRVDEKQKTEEQKERLTRMFAALSATNEAIMRAKSRVQLFE
                     MVCEAAANGGKFTSTTISLANRNSDFLDIAAVAGPTAETTRNVKVSISAARPEGRGLS
                     GAAFRSRQPCISNDYLADVNASAFHAIIRSDGAKSGAAFPLLIRGQAVGVMLFLSAEK
                     DTFTSEFVELLQRLADNVSFALENFDRADEKTKADARIEYLASHDSLTSLPNRETFNG
                     LLHHAIEAACRHQRRFAVLFIDLDRFKIINDSLGHDAGDILLVEIANRLRQTLRSSDV
                     VARLGGDEFVVILEETAESHDVERIAGNLLSVLSQPLQLSGHECHTTASIGIAIYPAD
                     GSDVQTLTKNADMAMYLAKEDGKNGFRFFTKEVKTQSIERLTLESALRRALERDQFAL
                     HYQPKVDMATGQITGVEALLRWTHPELGVLPPAQFIPLAEETGLIVPIGRWVLKEACA
                     QNMAWQRGGLRPVSMAVNLSPRQFVDEHLLKDIDEALAASGMPAVLLQLEVTESMVMR
                     NVSRAVKVLDAIQRRDIRLAIDDFGTGYSSMSLMKQFPIDTIKIDRSFVRDLPDDSED
                     RAIAQAIISMGKALGLTVVAEGVETAEQEIFLRDHACDEMQGFLFSKPLPPPQVADLL
                     RSASLVVAPPLQPAASAAPEKSTPRSRRRSAVV"
BASE COUNT          944 a         1667 c         1709 g         1087 t
ORIGIN      
        1 ccggcgacct ctaccgcggc ggaggaaaaa taacgacaag acctgcgcag gagcacgagt
       61 ggtgatccgt tctagccggc tcagccttcg gaattggcag gaatcggaca gagacacatt
      121 tgccgccctc aacgccactc ctgaagtgat gtgggatttg ggaggctctt tggatcgcaa
      181 cgagagcgac gccaaatttg accgctacgt ggcggcgttc gaccaacacg gcttctgccg
      241 atgggctctc gaagatctgg acgggaaatt cctcggctct gccggcgtca tgccatcgcg
      301 gcccggccat ccgctcggac cccatgccga catcggctgg cgtcttgtgc gcccggcctg
      361 gggtaggggc tatgccaccg aggctgcgag agcatccttg cgggatgtct ttgagcgggt
      421 gaatttgaag gaagtgctcg cttacacctc ggcggataat gtccggtccc aagcggtgat
      481 caagcgattg agcatgcagc gtgccgcgac acttgattat tccgagccac tcggcaaagc
      541 agtgtggcac ggcttggtgt ggatggttcg acgggacgac tcactcgatc tggatcattc
      601 gaatgaccgc gcccgttaat cgatattcca ggtaacgtgt gggatgcgcg cacgtccttc
      661 tgccagattg ttggtcatgg attcttccgg aaggatcctg ctcttccgct tcgtccacaa
      721 gaacggcgct ctcgcaggtc aggattactg ggccacgccg ggcggcggcg tcaaggatgg
      781 agagacgttc gagcaggccg ccatccggga gctcaaggaa gagaccggca ttggcgtgca
      841 ggaaattggt cccgagatag ggcgaagaat atttgtgctt caactgccca acggcgagca
      901 cgtgatggcg gacgagcggt tttttctggt cagaaccggg gacacatcgc tgtcacggga
      961 cggatggacc gcgcaagaag cggacatcat gaccgaccat aaatggtggt ctcgggatga
     1021 actggcgcag acatccgaga cagtctggcc cgagaacctt gcgacaatgc tcgacgccgc
     1081 gatgaaccca tagcttaact ggccggcaac ggggaacgcc gtgccgtccc aacaccatcg
     1141 acggcaaccc acccctgacg cgttgccaat ggagcgcacg aagcgcacca ttgagacttg
     1201 ctagccgcac tcccccgagg cccatatggt tggtttcata cggccgccct gcgacgccgg
     1261 aatcattcgc gggacgccgg cggttgccgc ggcgctatcg caacggcgca agcaattgac
     1321 gctggcggcg acgatcctcg gctcgagcct cgcgcttatc gacggttcgg tggtgaatat
     1381 cgccctgccg gcgatccagc aggccctgca tgcggacgcg gcttccacgc aatggatcgt
     1441 caatgcctac ctgttgctgc tcggcgcgct ggtgctggtc gggggctcgg ctgccgatct
     1501 ttacggccgc cggcggattt ttcttctggg catcgcgata ttcacctcgg cctcgattgc
     1561 ctgcggactc tcacctgaca tcacggtgct cgtcgtcagc cgcgcggttc aaggtcttgg
     1621 cgccgcactg ctgatgccgg ccagtctggc gatgctcggc gccactttcg acgagcatga
     1681 gcgcagccat gcgatcggga tctgggcggg cgccggtgcg ctgacggcgg cggcgggtcc
     1741 cgtgctcggc ggctggctgg tcgatcaggt gtcgtggcgt gcgatcttcc tgatcaacgt
     1801 gccactggct attgccgcgg ccgggctcgc aatcttcttt gcctgcgaaa gccgcgacga
     1861 ccaggcaaaa ccgctggact ggaacggcgc agtcgcggtc gccatcgggt tggccgcgat
     1921 cacctggggt ctgggcgcga ttccatccct gggatttcgc gacaaggccg tgcttggcgc
     1981 gctgggagcg ggaatagcgt cgctcctgtc gttcgtcgcg atcgaagccc gctcccgcga
     2041 acgcgcgatg atgccgctgt cgctgtaccg ctcgcgcaat ttttccggcg ccaacatgct
     2101 gacgctgctg ctctacttcg ccctcggcgg cgcgctgtat tttctcccct tcggcctgat
     2161 acggctgggt ggctattccg cgacccaggc cggcgcggcg gtgctgccgt tcgcgctgat
     2221 catggggttt ggcgcatcgt tcgcgggcac ggtcgccgac cgtttcgggc cgcggctgtc
     2281 gttgaccgca gggcccatca tcgcggccgg cggattggcg ctgctcgcct ttgccgacct
     2341 tggaaaatcc tactgggccg gcgtgtttcc ggcgcttggg gtgttctcga tcggcatgac
     2401 cgtcacggtt ccgccgctga cgtcgacggt gatggcatca gtgggtgacg ctcacgccgg
     2461 tgtcgcttcc ggcgtcaaca atgccattgc ccgcgtcgcc ggtctccttg cagtcgcggc
     2521 gctcggcgcc gttttatttg ccagcttttc gcgtcacctt gccggaataa cgcccgctgg
     2581 cgccaacgac gcattgaatg cggtcctcgc tggacaggcc gaagtcattg ccggcgccac
     2641 ggcagcgttc gaccgcgcac tgcgcacggt gatgctggtg acggcagtct gcgctgcgct
     2701 cggcggcatc gctggctggc tgtgggtccg gccgtccgac cctgcggagc cgcgcaactt
     2761 ttcctgatat cgcggacaac cgtaaattcc gtcggcacgc gttgcgtcgg taccagatcc
     2821 gcgtatcatc gaggcgcgag gggatttcga tgcatcaggc cgcaaatgtg caattcggac
     2881 gcgccgtggg cgaattcgcc agttggcgcg ccgtcccgga agacgcgaga tcgccggcgc
     2941 cggcatggtg gtggggaccg gcgtttgagg tgcatggcgc gccgcaaccg atgccggcgg
     3001 agtggtgcgc aaagctggaa ttgcctgagg gcgcgactta cgcaacgggc gcgggggttt
     3061 ttctcaacgc gctcgccggc cagacgtcgc tgccctggcc cggcgaattt ccacgcaagg
     3121 cggggcattc ggacgtggcc tagcgcggtc aaccagcttg ctggagcgtt ttccagcgaa
     3181 gtggagaccg gttcgcgtca agaaaacgcg tcaattcaag aatccagagc cccgtttcga
     3241 ttctatcgaa acggggctct agacgaccgc gcttctccgt cttgatcgtg gggtggattt
     3301 ctcaggcgcg gcacttgccg caggctgaag cggcggagcg acgacaagcg atgcggaccg
     3361 gagcaggtcg gcgacctgcg gtggcggcag cggtttgctg aacaggaagc cctgcatttc
     3421 atcgcaggcg tgatcgcgca ggaagatttc ctgctcggcg gtttccacgc cttcggcgac
     3481 gaccgtcagc cccagcgcct tgcccatgct gatgatcgcc tgcgcgatcg cccggtcttc
     3541 ggaatcgtcc ggaagatcgc gtacgaaaga cctgtcgatc ttgatggtgt cgatcggaaa
     3601 ttgcttcatc agggacattg acgaatagcc ggttccgaaa tcgtcgatcg caagacgaat
     3661 gtcccgacgc tgaatggcgt ccagtacctt gaccgcccgg gaaacgttgc gcatcaccat
     3721 gctttcggtg acctcaagct gcagcagcac cgcaggcatg ccgctcgccg ccagcgcctc
     3781 gtcaatatcc ttcagcaagt gctcatccac gaactgcctc ggcgacagat tgaccgccat
     3841 cgatacaggc cgcaatccgc cgcgctgcca ggccatgttt tgcgcgcagg cttccttcag
     3901 gacccagcgg ccgatcggga cgatcaaccc ggtctcttcc gcaagcggta tgaattgagc
     3961 gggcggcaac acaccgagtt ctggatgcgt ccagcgcaac agcgcctcga ctccggttat
     4021 ctgtcccgtc gccatatcca ccttcggctg ataatgcagc gcgaattgat cgcgctcgag
     4081 cgcacggcgc agcgccgatt ccagcgtcag ccgctcaatc gactgggtct tgacctcctt
     4141 ggtgaaaaac cggaagccgt tcttgccgtc ttccttggcg agatacatcg ccatgtcggc
     4201 gttcttggtc agcgtctgta cgtccgagcc gtccgccggg tagatggcga tgccgatgct
     4261 ggccgtagtg tggcattcgt ggccgctcag ttgcaacggc tggctgagga ccgacaacag
     4321 attgccggcg atccgttcga cgtcgtggct ttccgcggtt tcctccagaa tgacgacgaa
     4381 ttcgtcgccg ccgagccgcg ccacgacatc gcttgaacgc agggtttggc gcagccggtt
     4441 ggcgatttcg accagcagga tatcgccggc gtcatgcccc agcgaatcat tgatgatctt
     4501 gaacctgtcg agatcgataa acagtaccgc gaatcgccgc tggtggcggc acgccgcctc
     4561 aatcgcgtga tgaagcaacc cgttgaacgt ttctcgattg ggcagactgg tcaggctgtc
     4621 gtgcgatgcg aggtattcga tccgcgcgtc tgccttggtt ttctcgtcgg cgcggtcgaa
     4681 attctcaagg gcaaacgaca cgttgtcggc cagccgttgc aggagttcga cgaattcgga
     4741 tgtaaaggtg tccttctcgg cggaaagaaa aagcatgacg ccaaccgcct gcccacggat
     4801 cagtagcgga aatgccgcac ccgacttggc cccatcgctg cgaatgatgg cgtggaaggc
     4861 actggcgttg acgtcagcca gatagtcgtt gctgatgcag ggctgccgcg aacggaacgc
     4921 agccccgctc agtccgcgtc cctcggggcg cgcggcgctg atcgagacct ttacgttcct
     4981 tgtcgtctcc gcggtcggcc ccgccacggc tgcgatgtcg aggaaatcgc tgttgcgatt
     5041 ggccaagctg atggtggtcg aggtgaattt gccgccattc gccgcggcct cacacaccat
     5101 ttcgaacaac tgtacccggg atttggcccg catgatcgct tcgttggtcg cactaagcgc
     5161 ggcaaacatt cgcgtcagtc gttccttctg ctcctcggtt ttctgctttt cgtcgacacg
     5221 atcgaagtta tcgagggcga acgaaacatt ttccgccagt cttccaagaa gctccaccag
     5281 atcgtcggtg aatgcgcctt catcgaggga aagaaacaga aggacgccaa tggccctgtc
     5341 gccatctttc agcaaaggaa agcttgcccc ggatcgcgtc ccgtcttctc ttgcaaggac
     5401 gtgccag
//
LOCUS       DATNOI010000312         5052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_618676,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000312 DATNOI010000000
VERSION     DATNOI010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618676"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="VN968_12205"
     CDS             complement(<1..147)
                     /locus_tag="VN968_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXL30036.1"
                     /translation="MGYKVAVVGATGNVGREMLNILDERKFPADEVVALASRRSMGVE
                     VSYGD"
     gene            694..1080
                     /locus_tag="VN968_12210"
     CDS             694..1080
                     /locus_tag="VN968_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30037.1"
                     /translation="MTWKFPVANKLAVLLAAGAVVAVGFDITPASAQSKPRYDDRGRP
                     YYGPSGPNVSYQQGPRTRIYVTKRSWLDAGTEVLPGDRKFNDYAFPPEIGYPSFGREN
                     RNRPIDRQPLNPPSDLGGYPQTFPLY"
     gene            complement(1201..2313)
                     /gene="leuB"
                     /locus_tag="VN968_12215"
     CDS             complement(1201..2313)
                     /gene="leuB"
                     /locus_tag="VN968_12215"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HXL30038.1"
                     /translation="MAIHKLLLLPGDGIGPEVMGEVKRLIDWLNGKGIAQFETEQSLV
                     GGAAYDASKQSITDQTMAKAMAADAVIFGAVGGPKWDSVAYEARPEAGLLRLRKELGL
                     FANLRPAVCYPALADASSLKREVVEGLDIMIVRELTGGVYFGEPKIITDLGNGQKRAI
                     DTQVYDTYEIERIARVAFDLARKRRNKLTSMEKRNVMKSGVLWNEVVTQVHAREYKDV
                     QLEHQLADSGGMNLVKWPKAFDVIVTDNLFGDMLSDIAAMLTGSLGMLPSASLGEVDV
                     KTTKRKALYEPVHGSAPDIAGKGLANPIAMIASFGMALRYSFDRGALADKLDAAIAAV
                     LANGLRTADIKSDGTTAVSTAQMGEAILKELQALHG"
     gene            2581..3780
                     /locus_tag="VN968_12220"
     CDS             2581..3780
                     /locus_tag="VN968_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HXL30039.1"
                     /translation="MHAPDRPQSYVHPARLILILSLAPTVGLGIGRFAYSLVLPDMRD
                     ALGWSYSAAGFMNTINAAGYLAGALMASRLIKRFGLSAAVRWGTLACVVSLALCAMSG
                     NFFVLSFARLLAGLGAAVGFVAGGALGAMIAQTRPARANFLLSLFYAGPGLGIVSSGL
                     IAPFMLQAFGPGSWWIVWWAMTALSVLMTIPLLLAPIDRSAGIADVAPAKFAILPVSI
                     YLAGYFLFGAGYIAYMTFMIAYVRDGGGGAAAQSLFWSLIGISAFATPWVWRGALALD
                     RGGLSTTIILGVNALGASLPMFGHSPLLLAISALVFGVAFFAVVGSTTAFVRFNYPPS
                     EWPAGIAAMTIAFGVGQTLGPIVVGAITDTLGNLSYALSLSAAMLALGAILAAFQGKL
                     VAKSHAP"
     gene            complement(3808..4569)
                     /locus_tag="VN968_12225"
     CDS             complement(3808..4569)
                     /locus_tag="VN968_12225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL30040.1"
                     /translation="MTNGGKRVAWVTGGGSGIGEAGAEALAADGWTVVVSGRRRDALD
                     AVVARIIQNGGKANAIPLDVSVAADVETTVEQILAKHGRIDLLVNSAGINVPKRRWAD
                     MELEGWNKLVDVNLNGVLYCMRAVLPAMRRQKDGSIINVASWAGRHVSKMPGPAYTTT
                     KHAVLALTHSFNMDECVNGLRACCLSPGEVATPILKLRPVVPSEEEQAKMLQPEDLGR
                     TIAFVASMPPRVCMNEILISPTHNRGFIQTPVSRD"
     gene            4646..>5052
                     /locus_tag="VN968_12230"
     CDS             4646..>5052
                     /locus_tag="VN968_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXL30041.1"
                     /translation="MNTVANANLFSRLFDSLDDPNRLAIETLDGQRISYGDLIARVGR
                     MANVLVARGVKPGDRVAAQIEKSVENLVLYLAVVRAGGVYLPLNTAYTLNELDYFITD
                     AEPSLVVCDPSKVDGIGAIAAKVEAKVETLGPDG"
BASE COUNT          846 a         1617 c         1546 g         1043 t
ORIGIN      
        1 gtcgccatag gacacttcga cgcccatgct gcggcgtgac gccagcgcca ccacctcgtc
       61 agccgggaat ttgcgttcgt cgagaatgtt gagcatttcc cgcccgacat tgccggtcgc
      121 tccgaccacc gcgactttgt aacccatcgt ccactctccg aaggaaaaat gcctcgtccc
      181 tgccctttcg gcggaaaagg gacgaggcag cgcttctatg cgagaaccgc cgttaagaca
      241 acgttatacc gcaatgcccg gggcaattga tccgatcagg aatgccccgc tttgccgaaa
      301 gatgatgcgg catgctggcg cacctgttgc ttatatgagt attccggccc tgcttgccct
      361 gtcagcatcc gtctgtgaca cgaattgctc gaaaaaacag cgacctagcg gaccttccgg
      421 catcccgggg ccaccccgtc ccgggggcgc aacgcggaaa tccgacgctg cgcgcgaaat
      481 gctgcctagc ctccaaatac ctccgaacaa tctcgacggg caggcaggcc agggtcgggt
      541 gcgcccgcca aggtgctggt tttcatgcaa ctttttcgct cgccgccgca ttatgtgatg
      601 tggtgtggcc caattgacct agtaacgatg tccccgcgac ggctaagatc gctcccaatc
      661 ggaaatggcg gtccagccgc ccgtgaggac gtgatgacct ggaaattccc tgttgcgaac
      721 aagcttgcgg tgttgctggc cgcgggcgct gttgttgctg tcggcttcga catcaccccc
      781 gcaagcgcgc agtccaagcc acgatatgac gaccgcggcc gtccctatta cggaccgagc
      841 ggcccaaacg tgtcctatca acaggggccg cggacccgga tctatgtgac aaaacgttcc
      901 tggctcgacg ccggcaccga agtgttgccc ggcgaccgca agttcaacga ttatgcgttt
      961 ccgccggaga tcgggtaccc gtcgttcgga cgcgagaaca gaaatcgccc gatcgaccgc
     1021 cagccgctaa acccgccgtc cgaccttggc ggctatccgc agaccttccc gctttattga
     1081 gcgtgcccaa attaaattga cgtcgtcatg gccgggcttg tcccggccat ccacgttttt
     1141 gacgcaccat caagaaaaaa gacgtggatg cccgcgacaa gcgcgggcat gacgaccttg
     1201 ttatccgtgc agcgcctgca attccttcag gatcgcttcg cccatttgcg cggtgctcac
     1261 cgcggtcgtg ccgtcggact tgatatcggc ggtgcgcagg ccgttcgcaa gcaccgcggc
     1321 gatggcggca tcgagcttgt cggcgagcgc gcccctatcg aaggaatagc gcagcgccat
     1381 tccgaatgaa gcgatcatcg cgatcggatt ggccaatccc ttgccggcga tgtcgggcgc
     1441 cgagccgtgc accggctcgt acagggcttt tcgcttcgtg gtcttgacgt cgacctcgcc
     1501 gagcgaagcc gagggcagca tgccgagcga gcccgtcagc attgccgcga tatcggagag
     1561 catgtcgccg aacaggttgt cggtgacgat gacgtcgaac gccttcggcc atttcaccag
     1621 attcatgccg ccagagtcgg cgagctgatg ctcgagctgc acatccttgt attcgcgcgc
     1681 gtggacctgt gtgaccacct cgttccacag cacgccggat ttcatgacgt tgcgcttttc
     1741 catcgaggtc agcttgttgc ggcgcttgcg cgccagatcg aacgccacgc gcgcgatgcg
     1801 ctcgatctcg taggtgtcgt agacttgggt atcgatcgcg cgcttctggc cgttgccgag
     1861 atcggtgatg attttgggtt cgccgaaata gacgccgccg gtgagctcgc gcacgatcat
     1921 gatatcgagg ccctcgacca cctcgcgctt caggctcgag gcatccgcaa gcgcgggata
     1981 acacaccgcc gggcgcaggt tcgcgaacag cccgagttcc ttgcgcaggc gaagcaaccc
     2041 tgcctcgggc cgcgcctcat aggcgacgct gtcccacttc ggcccaccca ccgcgccgaa
     2101 gatcacggcg tcggcggcca tcgccttggc catggtctga tcggtaatgc tctgcttgct
     2161 ggcatcatag gccgcgccgc caactagact ctgctcggtc tcgaactgcg caatgccttt
     2221 tccgttgagc cagtcgatca ggcgtttgac ctcgcccatc acttcggggc cgatgccgtc
     2281 gccgggaaga agcagcagtt tatggatcgc catgggaagg tccttgttac gaattctcta
     2341 cttatcactc cggacgccga gccaacgggt ccggtcttcg ccggcccgat gacaggctcc
     2401 acgaggcgat ccggaatcca gcggtgagat ggattccggg ttcgcgccaa ggcgcgcccc
     2461 ggaatgacga atgcggagtg ctagtgcggc attgcgcgga tggcaagaca ccattgctta
     2521 ccgagcgcct gtgcaagccc cgctttgact gccacaatgc ggctcagccg gagtgatctc
     2581 ttgcacgccc ccgaccgtcc gcagtcttac gtgcatcccg cgcgtctgat cctcatcctg
     2641 tcgctcgcgc cgacggtcgg tctcggcatc ggccgctttg cctattcgct ggtgctgccg
     2701 gacatgcgcg atgcgctcgg ctggtcatat tccgccgcgg gcttcatgaa caccatcaat
     2761 gctgcgggtt atcttgccgg cgcgttgatg gcctcaaggc tcatcaagcg atttggcttg
     2821 tcggccgcgg tacgctgggg aacgctggct tgcgtggtgt cgctggcgct gtgcgccatg
     2881 tcgggcaatt ttttcgtttt gagctttgcg cggctgctcg cgggcctggg agcggcggtg
     2941 ggatttgtcg ccggcggcgc gctgggcgcg atgatcgcgc aaacacgacc cgcgcgggcc
     3001 aactttttgc tgagcttgtt ctatgccggc ccggggctcg gcatcgtgtc gtccggcctg
     3061 atcgcaccgt tcatgctgca ggctttcggg ccgggatcct ggtggatcgt ctggtgggcg
     3121 atgacggcgc tctcggtgct catgacgatt ccgctgctgc tggcgccgat cgacagaagc
     3181 gccgggatcg ccgatgtggc gcccgccaaa ttcgcgattc tgcctgtatc gatctatctc
     3241 gccggctatt ttctgttcgg cgccggctac atcgcctaca tgaccttcat gatcgcctat
     3301 gtgcgcgacg gcggcggtgg ggctgccgca cagagcctgt tctggagcct gatcggcatc
     3361 agcgcattcg cgacgccgtg ggtgtggcgg ggcgcgctgg cgctcgaccg tggcgggctg
     3421 tcgacgacca tcattctcgg cgtcaacgcg ctcggcgcga gcctgccgat gttcgggcat
     3481 tcgccgctat tgcttgcgat ctcggcgctg gtgttcggcg tcgccttctt cgcggtggtc
     3541 ggctccacca ccgccttcgt gcgtttcaat tatccgccat cggaatggcc ggccggaatc
     3601 gcagcgatga cgattgcatt cggcgtcggc cagacgcttg ggccgatcgt ggtcggcgcc
     3661 atcaccgaca cgctcggcaa tctgtcttac gcgctgagcc tgtccgccgc gatgctggcg
     3721 ctgggcgcga tactggcggc gtttcaggga aagttggttg cgaaatccca cgcaccgtaa
     3781 agcacacata catatggaga gatcgcgcta atccctgctc accggcgtct ggatgaagcc
     3841 tcgattgtgg gtcgggctga tcaggatctc gttcatgcag acgcgcggcg gcatgctggc
     3901 gacgaaggcg atggtgcggc cgagatcctc cggctgcagc atttttgcct gctcctcctc
     3961 ggacggcacc accgggcgca atttcaggat tggcgtggcg acctcgcccg gcgacaggca
     4021 gcacgcgcgc aagccgttga cgcattcgtc catgttgaag gaatgggtca gcgccagcac
     4081 cgcatgcttg gttgtggtgt aggccgggcc cggcattttc gacacgtggc ggccggccca
     4141 ggacgcgaca ttgatgattg agccatcctt ttgccggcgc atcgcgggca gcaccgcgcg
     4201 catgcaatag agcacgccgt tcaggttgac atcgaccagc ttgttccagc cctccagttc
     4261 catgtccgcc catcgccgct tcggcacgtt gatgccggcg ctgttgacga gaaggtcgat
     4321 gcggccatgc ttggccaaaa tctgttccac ggtcgtctcg acatcggcgg cgacgctgac
     4381 gtcgagcggg atggcgttgg ctttgccgcc attttgaatg attctagcaa ctacagcatc
     4441 gagcgcgtct ctgcggcgtc ccgacaccac aaccgtccag ccatccgccg cgagcgcctc
     4501 ggcgccagcc tcgccgatcc cgctgccgcc gccggtcacc caggccacgc gtttcccgcc
     4561 atttgtcatg caaactgtct ccgttgcttt tcagagggcg tttttgctat tgcagcatcc
     4621 cggcaccgcc cttggggaat gttgcatgaa caccgttgcc aacgccaatc tgttttctcg
     4681 cctgttcgat tcactcgacg atccgaaccg gcttgcgatc gaaactctcg atggccaacg
     4741 catctcttac ggcgatttga tcgcgcgcgt gggccggatg gcgaatgtgc tggtggcgcg
     4801 cggcgtgaag cctggcgacc gcgtcgccgc ccaaatcgaa aaatcagtcg aaaatctggt
     4861 gctctatctg gcggtcgtgc gcgccggcgg ggtctatctg ccgctcaaca ccgcctatac
     4921 gctgaacgaa ttggactatt tcatcaccga cgcggaaccg tcgctggtgg tgtgcgatcc
     4981 ctcgaaggtc gatggcatcg gcgcgatcgc ggcgaaggtc gaggccaagg tcgaaactct
     5041 cggtcccgat gg
//
LOCUS       DATNOI010000313        14111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_15955,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000313 DATNOI010000000
VERSION     DATNOI010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 14111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14111
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15955"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2825)
                     /locus_tag="VN968_12235"
     CDS             complement(<1..2825)
                     /locus_tag="VN968_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL30042.1"
                     /translation="MAPGLADNREVRPGSLSVVRPVASGAQGSLETFVKITADGSVIA
                     FNGHVDLGTGIRTALGQIVAEELDVSFARVVVVLGDTSRVPNQGATIASETIQITAVP
                     LRKAAAQARHFLVARAAERLELRVEDLAIEDGLIRGKDNRSVSYGELIAGDTIRLELA
                     DDVPVKAVNAYTIVGQSVPRIDLPAKATGETVYVHDVRVPGMLHGRVVRPPYGGVDAG
                     AFVGNSLIAVDEASVRDIPGLVAVVRIGDFVGVIAEREENAVKAAAQLRVSWKPVPTL
                     PDLKDVETALRANPSTPRTLIDKGDVDAAIAAAAKPIHRTYVWPYQMHGSIGPSCAVA
                     DYQAGHVRVWSGTQNPHLLRADLALLLLRPETDIDVIRMEAAGCYGRNCADDVSADAV
                     LLSRAAGRPVRVQLTREQEHAWEPKGTAQLMDVKGGLRADGSVAGYDFATRYPSNGAP
                     TLALLLTGMIEPVPAVFEMGDRTAIPPYDYENIRVVAHDMPPIVRASWFRGVSALPNT
                     FAHESYIDELATEAGVDPIEYRLRYLKDPRAIDLVKAVTKRAGWIPRPAWKEPVAEGD
                     IVRGRGFAYALYIHSQFPGYGAAWSAWIADVAVNKATGDVSVTRVVAGQDSGLMINPD
                     GVRHQIHGNVIQSTSRALMEEVSFDRNSVTSREWGAYPIITFPDIPKIDVLMLPRQDQ
                     PPLGVGESASVPSAAAIANAIFDATGVRFRELPFTPERILRGLRGEQDAAQDASSLAA
                     PKPPGPIDKWKNPLAGRRGALATAAALCAAVVGVAAAVLPWRSIAPITRPDASVYSAA
                     TIARGRQLSALGDCAVCHTEANGARNAGGRPLATPFGIIYSTNITPDLETGIGAWSYP
                     AFERAMREGIHRDGRHLYPAFPYTHFAKVTDADLQALYAYLMAQAPVRADTPSNALAF
                     PFNWRPLMAGWNALFRQPGVFEADATKS"
     gene            complement(2825..3307)
                     /locus_tag="VN968_12240"
     CDS             complement(2825..3307)
                     /locus_tag="VN968_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL30043.1"
                     /translation="MRQPTIRLTVNGGIHDVNADPDTALLYVLRNDLELNGPKYGCGL
                     GECGACAVLIDDVAARSCVIPISGCVDRDIVTLEGLGSRDHPDPVQQAFIAEQAAQCG
                     YCLNGMIMSTKALLLRSPHPSETELLEALRYNLCRCGAHIEIMRAAMRAAGALAEAQD
                     "
     gene            3651..5111
                     /locus_tag="VN968_12245"
     CDS             3651..5111
                     /locus_tag="VN968_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxynicotinate reductase"
                     /protein_id="HXL30044.1"
                     /translation="MLTDTTTAPVDDKIRCDACPVMCYIKPGAAGACDRYANHNGELV
                     RVDPHIVLERTVSHGGRLVPFQAGGDWDGKIVHEPSVFVTAIGAGTTYPDYKPAPFIV
                     SSEIDGVDMVTVVTEGIFSYCGVKVKIDTDRYLGPETAMVRAHGEAVGHVTTSEYGSQ
                     MLSLGGVHHLTGGSKKEGRVTCDTLMDLSNCKPVELTIDGGATVVVQAGYPPIVNGVP
                     EERMRVGCGSATIGMFAKQWHGKVDEVVVVDDHITGVLSEHQAGKLLDIPDTGIKMKG
                     RRSTPGRYFQVAEPGTGWGGTKISDPLSVLGPFDPKKAHPGMTMLMVSTTGEHAAYYE
                     LDEALKPVEKEMPADLKHSVERIQENCEPALCTVLFMGGAGGSLRAGVTDNPVRLTRS
                     VKEALTRVTSGGAPVYVWPGGGITFMVDVTQLPAGAFGYVPTPALVAPIEFTLKLSDY
                     AALGGHMDHVRPLASLKDNSEFRHTPSLQLSPGQRA"
     gene            5108..6019
                     /locus_tag="VN968_12250"
     CDS             5108..6019
                     /locus_tag="VN968_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HXL30045.1"
                     /translation="MTRAPQIALLADRRRLHLQDGPIDLIVEARGSEANVRAAYDAAV
                     QRFTGLLDELCEELPLLRQAADPARCPLQGAVARRMHAAVAPFASQHFITPMAAVAGS
                     VAEEILGAMLQKAPLGRAYVNNGGDIALHLDDGEHFTVGLAERPDEPGAMPTMVIHAD
                     DPTRGVATSGRHGRSFSLGIADAVTVLARTASRADAAATIIANAVDLPGHPAIVRCPA
                     HDLQPDSDLGARLVTRGVGELSADEIEVALESGAACARKLLAAGLIDGAALRLHSEMV
                     TVATRTIEMSNAQARLRGHAMERAMHV"
     gene            6053..6637
                     /locus_tag="VN968_12255"
     CDS             6053..6637
                     /locus_tag="VN968_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HXL30046.1"
                     /translation="MSAIIRKIVTVVEETHREMGQTVTPPTRRAAAIAVIENPFAGKY
                     VEDLSPLIAIGEELGELLSKKAVAALGIDGAKAQSYGKAAAVGENGELEHAAAILHPK
                     MGAPVRKVLGKGAALIPSSKKRSGPGTTLDIPLGHKDAAFVRSHFDGMEVQINDAPRA
                     NEIMVAVAITDSGRPLPRVGGLTIAEIKGEDGLR"
     gene            6659..7864
                     /locus_tag="VN968_12260"
     CDS             6659..7864
                     /locus_tag="VN968_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30047.1"
                     /translation="MRRSRYFLGAGLAIMIGGMAHSAVAQSEIKIGEINSYSLLPAFT
                     EPYRKGWQLALEEVNAAGGINGKKLVVVSKDDGGKPADAQTAANELVSSENVVMLTGT
                     FLSNIGLAVSDFANQKKVFFLAAEPLTDAITWAKGNRYTFRLRPSNYMQAAMLVEEAA
                     KLPAKRWATVAPNYEYGQSAVAVFKKLMSEKRPDIQWVDEQWPPQGKIDAGPVVQAVA
                     SANPEAILNVTFGADLVKLVREGNTRGLFNGRSVVSFLTGEPEYLDPLKDETPEGWIV
                     TGYPWYGIKMPEHDAFLKAYQAKYNDYPRLGSIVGYQTIKSAAAILAKANSTDPEKLI
                     AATEGLSVASPFGEITFRKIDHQSTLGAFVGKTALKDGKGIMVDSVYRKGMDYLPSDA
                     EVDKLRPKD"
     gene            8047..9930
                     /locus_tag="VN968_12265"
     CDS             8047..9930
                     /locus_tag="VN968_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL30048.1"
                     /translation="MAFYFVQFLTGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYM
                     LGAYVAFTLTERFSGAIGFWGGIFAAGLIVAAVGVLVEMVLLRRIYHAPELFQLLATF
                     GLTLMVEDLVVLIWGPSDLLGRRAPGFKGAVDFFGQNIPTYDLFLIGLGPVVLGVLWL
                     MFQRTRWGVLVRAATQDRDMVAALGVNQKWLFTSVFALGVFLAALGGGLQIPRDAVHH
                     ALDLRIIVDVFVVVVIGGLGSIIGAFVAAVLVSELNAFGILIFPKISIILVFLVMAAV
                     LIVRPWGLFGKQEAAARRTPGLTVNPWRPLTSGERLTAMAALALAAALPLIGGNYALT
                     VGSEIAIFVIFAASLHFLMSVGGLASFGHAAYFGLGAYGVAFLAKLAGLPMIACLLLG
                     PLLGCLGAAVFGFFAVQLSGVYFAMLTLAFAQIVWSIAFQWVAVTGGDNGILGVWPER
                     WAAPPSHFYWLSLGVAALVVAALRIIVFSPFGFALRATRDSPLRSEAIGIDGKRIQWT
                     AFVIAGTVAGIAGALFAYLKGSVFPDSLGISLSVDALVMVLLGGVETVSGAVIGAIVY
                     KALSIWLVSQTDLSKLVLGAVIVLIVVAFPKGIVGMLETIRNRRTQSSKSALLGSRIE
                     IAE"
     gene            9927..10718
                     /locus_tag="VN968_12270"
     CDS             9927..10718
                     /locus_tag="VN968_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30049.1"
                     /translation="MSITPTLLSVENLSKSYGGVHAVRGVSFALHAGEILALIGPNGA
                     GKSTCFDMLNGQNNPDSGRINLLGQDTTGKKPRAIWRLGVGRTFQITATFPTMTVCEN
                     VQVALVSYDRQLFNLWASTPKFARTEAGRLLELVGMSGFAQRPCGELAYGDLKRLELA
                     IALANQPKLLLMDEPTAGMAPRERIELMRLTAQIARDQSIGVLFTEHDMDVVFEHADR
                     ILVLNRGTLIAEGSPEEVRRNPQVRAIYLGEGLVYDARHREGAGA"
     gene            10715..11419
                     /locus_tag="VN968_12275"
     CDS             10715..11419
                     /locus_tag="VN968_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30050.1"
                     /translation="MKLLVEGLNSYYGPAHILFDIALEVGQGEVVALLGRNGAGKSTT
                     FRSIVGLVEQRSGRIMFEGRDVSSQPTHAIVSGGLGYVPEERRIFTDLTVDENLEVGR
                     QPVRSGAPHWTKEKLFTLFPNLAEMRGRHGGRMSGGEQQMLTIARTLMGNPSLVLLDE
                     PSEGLSPKIVEQMVEAILTMKKEGVGIVVSEQNLHFARLISDRAYIIERGRICFNGTM
                     RELDARPDIRDAHLAL"
     gene            11456..11911
                     /locus_tag="VN968_12280"
     CDS             11456..11911
                     /locus_tag="VN968_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXL30051.1"
                     /translation="MTRSVSAKRNVKPSRPAYVLEEQIGFILRQVWQRHATIFAREIG
                     INLTPTQWAALAKLTETGPCSQNQLGRLTAMDVATIKGVIDRLTARGLTETSPDPEDG
                     RRLLVSLTRAGQQLAEKAAPNALAITKETLAPLDARERETLVALLSKLR"
     gene            12035..13045
                     /locus_tag="VN968_12285"
     CDS             12035..13045
                     /locus_tag="VN968_12285"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30052.1"
                     /translation="MAFRIKRREFIGLIGGATVAWPFVALAQKPDRSRRIGVLMSLGE
                     NDPEGKAQLSGFTQGLADLGWSDGPNLRMDVRWGGGDVNKIRTFAKELVAAQPDLILS
                     QGTPVTAALQRETRTIPIVFVVVTDPVGEGFVAGLPLPGGNITGFITSEAAMGSKMLD
                     LLLDIAPGINRVAMIFNPDTAPGGGKYYLGDFEAAARSSRIAPIAARAQSDAEIDTVV
                     TSLGREPDGGLVVMPDFFMLNHVQPILLQAARSNVPTIYPWRYVVTKEAGLISYGPDF
                     RDIVRRAAPYVDRILRGAKPADLPVQVPVKFEMAVNVKTAKALGLTVPTSLLLRADEV
                     IE"
     gene            complement(13119..13364)
                     /locus_tag="VN968_12290"
     CDS             complement(13119..13364)
                     /locus_tag="VN968_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXL30053.1"
                     /translation="MYPRRFARVRPAGKGSSSAKLIVGPRDPLIDCTVVDYSPGGACL
                     EVFGQPRLPNRFELLFGGTKKRCRVVWSAGRRLGVAF"
     gene            complement(13550..13852)
                     /locus_tag="VN968_12295"
     CDS             complement(13550..13852)
                     /locus_tag="VN968_12295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HXL30054.1"
                     /translation="MHKMLVLYNDPKDPAHFRKYYVETHLPLVSKLPNLKASRYSFDV
                     KSLGPGKAPYFCIFEAEFENEAALMAALGSKEGKAVAGDVPNYASGGVTMMHFAVG"
BASE COUNT         2630 a         4290 c         4519 g         2672 t
ORIGIN      
        1 gacttcgttg cgtcagcctc gaacacacct ggctggcgaa acagcgcgtt ccaacccgcc
       61 atcagcggac gccagttgaa cggaaacgcc aacgcgttgc ttggagtatc cgcgcgcacc
      121 ggcgcttggg ccatcaggta agcatagagc gcctgcaggt cggcgtcggt taccttggcg
      181 aaatgcgtgt aaggaaatgc cggatagaga tgccttccat cgcgatggat gccctcgcgc
      241 atggcgcgtt cgaatgcggg ataggaccag gcgccgatcc cggtctcgag atcgggtgtg
      301 atgttggttg agtagatgat cccgaacggc gtcgcgagag gtcggccgcc agcgttgcga
      361 gcaccattcg cctcggtgtg acaaaccgcg cagtcgccga gtgcggaaag ctgtcggcca
      421 cgggcaatgg tggcggccga atacaccgac gcatcgggcc gcgtgatcgg cgcgatcgag
      481 cgccacggca atacggccgc ggcgactccg accaccgcgg cgcataatgc tgcggcggtt
      541 gccagcgcac cacgccggcc ggcaagcgga tttttccatt tgtcgatcgg gcccgggggc
      601 ttcggtgccg ctaacgacga tgcgtcttgt gctgcatcct gttcgccgcg caatcccctg
      661 aggattcgct ccggcgtgaa cggcaattcg cgaaaacgca cgccggtcgc gtcgaagatt
      721 gcattggcga tcgccgcggc gctcggaacc gaagcggact cgccgacgcc aagcggcggc
      781 tgatcctgcc gcggcagcat caagacgtcg atcttgggaa tgtccgggaa agtgatgatg
      841 gggtaggcgc cccattcgcg gctcgtcacc gaattgcgat cgaatgagac ctcctccatc
      901 agcgcacggc tggtcgactg gatgacgttg ccatggatct ggtggcggac cccgtcggga
      961 ttgatcatca ggccggaatc ctggccggcc accacgcggg tcacgctgac atcgccggtc
     1021 gccttgttga cggcgacgtc ggcaatccag gccgaccacg ccgcgccgta gcccggaaac
     1081 tgactgtgga tatagagcgc gtaggcaaag ccgcgcccgc ggacgatgtc accttcggcg
     1141 acaggttctt tccatgccgg gcgagggatc cagccggccc gcttggtcac agccttgacc
     1201 agatcgatcg cgcgcggatc tttgagatag cgcaggcgat attcgatcgg atcgacgccg
     1261 gcttcggtgg ccagttcgtc gatgtaggat tcatgcgcga atgtatttgg gagcgccgag
     1321 accccacgaa accacgatgc gcgcacgatc ggtggcatgt cgtgcgcaac cacgcggatg
     1381 ttttcgtagt cgtaaggcgg gatcgcggtg cggtcgccca tctcgaacac ggcagggacc
     1441 ggctcaatca tgccggtgag cagcagcgcc agggtagggg cgccgttgga gggatagcgt
     1501 gtcgcaaaat catatccggc gacgctgccg tcggcgcgca gtccgccctt gacgtccatc
     1561 agctgcgcgg tgcccttcgg ctcccaggcg tgttcctgct cgcgcgtcaa ttgcacgcga
     1621 accgggcggc cggccgcgcg cgacagcaga actgcatcgg ccgagacgtc gtcggcgcag
     1681 ttgcgcccat agcagccggc ggcctccatc cggatcacgt cgatgtctgt ttccgggcgc
     1741 agcagcaata gcgcgaggtc ggcgcgcaaa aggtggggat tttgtgttcc ggaccagacg
     1801 cggacgtggc cggcttgata gtccgcgacg gcacaggacg ggccgatcga accatgcatc
     1861 tggtagggcc acacataggt ccgatgtatc ggctttgcgg cggcggcgat cgccgcatcg
     1921 acgtcgcctt tgtcgatcaa cgtccgtggc gtcgacggat tggcgcggag cgccgtctcg
     1981 acgtccttca gatcgggcag cgtcggcaca ggcttccagc tcaccctgag ctgcgccgcc
     2041 gccttgacgg cgttctcctc gcgctcggcg atgacgccga cgaaatcgcc gatcctgacg
     2101 accgcgacca gccccgggat atcgcgcacc gaagcttcat cgacggcgat caaactgttg
     2161 ccgacgaagg cgccggcatc gacgccgccg tagggcgggc gcacgacgcg gccgtgcagc
     2221 ataccgggca cgcgcacgtc atgaacatag acggtttcgc cggttgcctt cgccggcaga
     2281 tcaatgcgcg gcaccgactg cccgacgatg gtgtaggcgt taaccgcctt caccggaacg
     2341 tcgtcggcca attcaagccg gatggtatcg ccggcgatta attcaccata gctgacgctg
     2401 cggttgtcct tcccgcggat caggccgtcc tcgatcgcaa gatcctcgac gcgaagctcg
     2461 agccgttccg ccgcgcgagc gaccaggaaa tgccgcgcct gtgcagcggc cttacgcaag
     2521 ggcacagccg tgatctggat ggtttcgctg gcgatggtcg cgccctggtt ggggacgcgc
     2581 gaggtatcgc ccagcaccac gacgacgcgc gcaaaggaca cgtcgagttc ctcggcgacg
     2641 atctgtccaa gcgcggtacg aatgccggtg ccgagatcga catggccgtt gaacgcaatg
     2701 accgaaccgt cggccgttat tttcacaaaa gtttcgagcg agccttgcgc gccggaagca
     2761 accgggcgga cgaccgacag cgatccgggc cgaacctcac gattgtcggc aagccctggc
     2821 gccatcaatc ttgcgcctcg gcgagagcgc ctgccgcccg cattgccgcg cgcatgattt
     2881 cgatatgagc gccgcatcgg cacaaattgt agcgcagcgc ttccagcagt tcggtctccg
     2941 atgggtgagg gctacgcagc aacagtgcct tggtgctcat gatcatgccg ttgaggcagt
     3001 agccgcattg cgcggcctgt tcggcgatga aggcctgctg caccgggtcg ggatgatcgc
     3061 gcgagccgag gccctcaagc gtcacaatgt cgcggtcgac gcagccgctg atcgggatga
     3121 cgcaggaacg tgccgcgacg tcgtcgatca gcaccgcgca ggccccgcat tcgccgaggc
     3181 cgcagccgta tttcgggccg ttcaattcga gatcgttgcg caacacgtaa agcagcgcgg
     3241 tatcgggatc agcgttgacg tcgtggatgc cgccgttgac ggtcaggcgg atcgtgggct
     3301 gcctcatgct gctggttccc cgcccgctgg ttgcgctgca aaacatcgcg ataaaccctt
     3361 cgcgaagata ccgtttgcat acaaacgtgc aagccggcca tcccgagcga agcggtctgc
     3421 ccggtttgta cacattgccg cagggcaatt gaacttttat tcggcagcgg gctctttcgc
     3481 ttccaaacac gcttgacagt ttttcggcac gcgcgcaaca tcgtttgtat acgaacaaaa
     3541 tgctggcggc gagagatatg atattgtgga agcgcctgaa ctctcgttga tgatacgttg
     3601 ctgccaattg gcttatccga cagtctgacg ctggctttga gagcgtgttc atgctgactg
     3661 atacgacaac cgctcccgtc gacgacaaga tccgctgcga tgcttgtccg gtgatgtgct
     3721 acatcaagcc gggggccgcg ggcgcctgcg atcgctacgc caaccacaat ggcgaactgg
     3781 tgcgcgtcga ccctcatatc gtcctggagc gaaccgtgtc gcatggcgga cggctggtgc
     3841 cgttccaggc cggcggcgac tgggacggca agattgtcca cgagccaagc gtgttcgtga
     3901 ccgcgatcgg cgccggcacc acctatcccg actacaagcc ggcgcccttc atcgtctcgt
     3961 cggagatcga cggggtcgac atggtcaccg tggtgaccga aggcatcttc agctactgcg
     4021 gcgtcaaggt gaagatcgac accgaccgat atctcggtcc ggagacggca atggtgcggg
     4081 cgcacggtga ggccgtgggt catgttacca ccagcgaata cggctcgcag atgctgtcgc
     4141 tgggcggcgt gcatcatctc accggcggct caaagaaaga aggccgcgtc acctgcgaca
     4201 cgctgatgga tttgagcaac tgtaagccgg ttgaactgac gatcgacggc ggtgcaacgg
     4261 tggtggtgca agctggatat ccgccgatcg tgaacggtgt tcccgaggaa cgcatgcggg
     4321 tcggctgcgg ctcggcgacc atcggcatgt tcgcaaagca atggcacggc aaggtggatg
     4381 aggttgttgt cgtggatgac cacatcaccg gcgttctttc ggaacatcag gccggcaagc
     4441 tactcgatat tcccgacacc gggatcaaga tgaaagggcg gcggtcgacg cctggccgtt
     4501 atttccaggt ggccgagccc ggcactggct ggggcggcac caagatctcc gatccactct
     4561 cggtgcttgg tccgttcgat ccgaagaagg cgcatcccgg catgaccatg ctgatggtct
     4621 caacgaccgg ggagcatgcg gcttactatg agctcgacga agcgctcaaa cccgtcgaga
     4681 aagagatgcc ggccgatctc aagcactcgg tcgagcggat ccaggagaac tgcgaaccgg
     4741 cactgtgtac tgtgctgttc atgggcgggg cgggcgggtc gctgcgggcc ggcgtcaccg
     4801 acaatccggt ccggctgacg cgatcggtca aggaggcact gacgcgcgtc accagcggcg
     4861 gcgcgccggt ttatgtctgg cccggcggcg gcatcacctt catggtcgat gtcacgcaat
     4921 tgccggcagg cgccttcggc tacgtgccga cgccggcgct ggtggcgccg atcgagttta
     4981 cgctaaaatt gtcagactat gcggcactgg gcggccacat ggatcacgtg cggcccctgg
     5041 cgtcgttgaa ggacaatagc gaattccgtc acacgccttc gctgcaactt tctccaggac
     5101 aacgcgcatg acgagggcgc cccaaattgc gcttctggcc gaccgcaggc ggcttcatct
     5161 gcaggacggc ccgatcgatc tgatcgtgga ggcgcgcggc agcgaggcaa atgtgcgcgc
     5221 ggcctatgat gccgcggtac agcgttttac cggtttgctc gatgagctct gcgaggagtt
     5281 gccgctgctg cgccaggcgg ccgatccggc gcggtgcccg ctgcaaggcg cggtcgcgcg
     5341 ccgcatgcat gcagcggtag cgccgtttgc gtcacaacat ttcattacgc cgatggcggc
     5401 ggtcgcgggc tccgtcgcgg aagaaattct cggcgccatg ctgcagaagg caccactcgg
     5461 gcgcgcttac gtcaacaatg gcggcgacat cgcgctgcat cttgacgacg gggaacattt
     5521 tacggtgggt ctggcggagc ggcccgacga gcctggcgcg atgccaacca tggtcattca
     5581 tgccgacgat cccacccgag gggttgccac cagcggccgc cacggccgca gtttttcgct
     5641 tggcattgcc gacgccgtca ccgtactggc acggaccgcc tcgcgagccg acgccgccgc
     5701 caccattatt gccaatgccg tcgatttgcc cggacatccc gccatagtcc gctgtccagc
     5761 tcatgatctg caacccgaca gcgatctcgg cgcgcgactg gtcacccgcg gcgtcggcga
     5821 attgtcagcc gacgagattg aggttgcgct cgaatccggc gcggcttgcg cgcgaaagct
     5881 gcttgccgct ggcttgatcg acggtgccgc attgcggttg catagcgaaa tggtcacggt
     5941 ggcgacgagg acgatcgaga tgtcgaacgc gcaggcgcgg ctgcgcgggc acgccatgga
     6001 aagagcgatg catgtttaag ctcacctaca tcagacagcg gggcggacaa cgatgagcgc
     6061 gatcattcgc aagatcgtca cggtggtcga ggagacgcat cgggagatgg gtcagaccgt
     6121 caccccgccg acccggcgcg cggcggcgat cgccgtgatc gaaaatccat tcgccggaaa
     6181 atacgtcgaa gacctttcac cgcttattgc gatcggtgag gaactcggcg agctgctttc
     6241 gaaaaaggcg gtcgccgcgc tcggcatcga cggcgccaag gcgcaaagct acggcaaggc
     6301 ggcggccgtc ggcgaaaatg gcgagctcga acatgcggcc gcgatcctgc atccgaagat
     6361 gggcgcgccg gtgcgcaagg tgctgggcaa gggcgcggcg ctgattccgt catcgaagaa
     6421 gcgcagcggg ccgggcacca cgctcgacat cccgcttggc cacaaggacg cagccttcgt
     6481 gcgcagccat ttcgacggca tggaagtgca gattaacgat gccccgcgcg ccaacgagat
     6541 catggtggcg gtcgcgatta ccgacagcgg caggccgctg ccgcgtgtcg gcggcctgac
     6601 gattgcggaa atcaagggcg aggacggtct acgatagttt caacagtgga ggtaagggat
     6661 gcggcggagc agatattttc tgggcgcggg actggcgatc atgattggcg gcatggcgca
     6721 ttccgccgtg gcgcagagcg agataaagat cggcgagatc aacagctatt cgctgttgcc
     6781 tgcttttacc gagccctatc gcaagggctg gcagctcgcg ctcgaagaag tcaatgccgc
     6841 cggcggcatc aacggcaaga agctcgtcgt cgtctccaag gatgatggcg gcaagcccgc
     6901 cgacgcgcag accgcggcca acgaactggt gtcgagcgag aatgtggtga tgctgaccgg
     6961 caccttcctt tccaatatcg gtctggcggt gagcgatttc gccaatcaga aaaaggtttt
     7021 cttcctggcg gccgagccgc tgaccgatgc catcacctgg gccaaaggca accgctacac
     7081 tttccgcctg cgtccatcca actacatgca ggccgccatg ctggtggaag aggcggcaaa
     7141 actgcccgcg aaacgctggg cgacggtcgc gccgaattat gagtatgggc aatcagcggt
     7201 tgcggtattc aagaaactga tgtcggaaaa acggcccgac atccagtggg tcgacgaaca
     7261 atggccgcca cagggcaaga tcgacgcggg tccggtcgtg caggccgtcg catccgccaa
     7321 tccggaagcc attctcaacg tcaccttcgg cgccgacctg gtaaagctcg tgcgcgaagg
     7381 caacacccgg ggactgttca acggtcgctc ggtcgtctct ttcctcaccg gcgagcccga
     7441 atatctcgac ccgctgaagg atgaaacgcc ggagggttgg atcgtcaccg gctatccctg
     7501 gtacggcatc aagatgcccg agcacgatgc tttcctgaag gcgtatcagg ccaagtataa
     7561 tgactatccg cggctcggct cgatcgtcgg ctatcagacc atcaaatcgg ccgcggcgat
     7621 cctcgccaag gcaaattcga ccgatccgga aaaactgatc gccgcgacgg aagggctgtc
     7681 ggtggcatct ccgttcggcg agatcacctt ccgcaagatc gatcatcagt ccacattggg
     7741 cgcgttcgtc ggcaagaccg cgctgaagga cggcaaaggc atcatggtgg attcggtcta
     7801 tcgcaagggc atggactatc tgccaagtga tgccgaggtc gacaagctgc gcccgaagga
     7861 ttgaggcttt agagctattc cggttcgtca gaatgatgac cgggatcggg gaccgtgtgg
     7921 ttcgagacgc gcggcgctgc cgcgctcctc accatgagga tctagacctc atcctgagga
     7981 ggcgcgacgc gtcgtctcga aggatgaagc cactgcactg aaaagcgcgc tggagcggat
     8041 gcgcaaatgg ccttttactt cgttcagttc ctgaccggtc ttgccagcgc ggcgtcgctg
     8101 tttctggtgg cgtcgggact gtcgatcatt ttcggcgtga cacgcatcgt caatttcgcg
     8161 catggcgcgt tctacatgct cggcgcttac gtcgcgttta cgctgaccga gcgattttcc
     8221 ggcgcgatcg gcttctgggg cggcattttc gcagcagggt tgatcgtggc agccgtcggc
     8281 gtgctggtcg aaatggtgct gctgcggcgg atctaccatg cgcccgaatt gtttcaactg
     8341 ttggcgacgt tcggcctcac tttgatggtc gaggatctgg tggtgctgat ctgggggccg
     8401 agcgacctgt tggggcgccg cgcgccggga tttaagggcg cggttgattt tttcggccag
     8461 aacattccaa cttacgacct gtttctgatc gggctcggtc cggtcgtgct cggagttctg
     8521 tggctgatgt tccagcgaac gcgctgggga gtgctggttc gcgccgcaac gcaggaccgc
     8581 gacatggtgg ccgcgctcgg ggtcaatcaa aagtggctgt tcacgagcgt gttcgcgctc
     8641 ggggtttttc tcgcggcgct cggcggaggc ttgcagattc cacgcgacgc ggtgcaccac
     8701 gcgctggatt tgcggatcat cgtcgacgta tttgtggtgg tcgtcattgg cggtctaggc
     8761 agcatcatcg gcgcgtttgt cgcagcggtg ctggtgtcgg agctgaacgc gttcggcatc
     8821 ctgatttttc caaagatctc catcatcctg gtgttcctgg tgatggccgc cgtcctgatc
     8881 gtgcggccat ggggactgtt cggcaagcag gaagccgcag cgcgccgcac gcccgggctg
     8941 accgtcaatc catggcgccc gctgacgtcg ggcgaacgat taacggcgat ggctgcgctg
     9001 gcgctcgcgg cggcgctgcc gctgatcggc ggcaactacg cgctcacggt gggctcggag
     9061 attgcgatct tcgtgatctt cgccgcgagc ctgcatttcc tgatgtcggt cggcgggctc
     9121 gcctcgttcg gccacgccgc ctatttcggg cttggcgcct acggcgtcgc gttccttgcc
     9181 aagctcgcag gcctgccgat gattgcctgc ctcctgcttg gcccgctgct cggatgcctc
     9241 ggtgcggcgg tgttcggatt ttttgcggtc cagctgtcgg gcgtctattt cgcgatgctg
     9301 acactggcat tcgcgcagat cgtctggtcg atcgcgttcc agtgggtcgc ggtgaccggc
     9361 ggcgacaacg gcatcctcgg cgtctggccg gagcgatggg cggcgccgcc ttcgcatttc
     9421 tactggcttt cgcttggagt ggccgcgctt gtggtcgcgg cgcttcggat catcgtgttc
     9481 tcgccctttg gctttgcgct gcgggcgacg cgcgactcac cgttacgcag cgaggccatc
     9541 ggcatcgacg gcaagcgcat ccagtggacg gcgtttgtga tcgccggcac cgtcgcgggc
     9601 atcgctggcg cgctgtttgc gtatctcaag ggcagcgtgt ttcccgacag cctcggcatt
     9661 tcgctctcgg tcgatgcgct ggtcatggtg ctgctcggcg gggtcgagac cgtctcaggc
     9721 gcggtgatcg gcgccatcgt ctacaaggcg ctctccattt ggctggtgag ccagaccgat
     9781 ctgtccaaac tggtgctggg cgccgtcatc gttctgatcg tggtggcttt cccgaagggt
     9841 atcgtcggca tgcttgagac gatccgcaat cgccgaactc aatcgtcaaa atccgcattg
     9901 cttggctccc ggatcgagat tgccgaatga gcatcacgcc cacgctgcta tcagtcgaga
     9961 acctgagcaa atcctatggc ggcgtccatg cggtgcgtgg cgtgtcgttt gcgcttcatg
    10021 ccggcgaaat cctggcgctg atcggtccga acggcgcggg caagagcacc tgcttcgata
    10081 tgctcaacgg ccaaaacaat cccgacagtg gccggatcaa tctgctcggc caggacacca
    10141 ccggcaagaa accgcgcgcg atctggcggc tgggcgtcgg acgaaccttt cagatcaccg
    10201 cgacattccc gaccatgacg gtgtgcgaga acgttcaggt cgccttggtg tcctatgacc
    10261 ggcagttgtt caatctctgg gcctcgacgc caaaatttgc gcgaacagaa gccggccgtc
    10321 tgctcgaact cgtcggcatg agcggttttg cgcaacgccc ttgcggcgag ctcgcctatg
    10381 gcgatctcaa gcggctggaa ctcgctatag ccctcgccaa tcagccaaaa ctgctgctga
    10441 tggatgagcc gaccgccggc atggcgccgc gcgagcggat cgagctgatg aggctcaccg
    10501 cacaaattgc gcgggatcaa tcgatcggcg ttctctttac cgagcacgac atggacgttg
    10561 tcttcgaaca tgccgaccgc atcctggtgc tcaatcgcgg cacgttgatc gccgagggct
    10621 cgcccgaaga agtccggcgc aacccgcagg ttcgcgccat ctatctcggc gaggggctgg
    10681 tttacgacgc gcgccatcgc gagggagccg gcgcatgaaa ctcttggtcg aggggctcaa
    10741 cagctattac ggccccgctc acatcctgtt cgacatcgcg ctggaagtcg gccagggcga
    10801 agtcgtcgcg ctgcttgggc gcaatggcgc cggcaagtcg acgacctttc gctcaatcgt
    10861 cggcctggtc gagcaacgct ctggccgcat catgttcgag ggcagggatg tctcctctca
    10921 gccaacccat gcgatcgtca gcggcggcct tggctacgtg ccggaggagc ggcgcatctt
    10981 caccgacttg accgtcgatg aaaacctcga ggtgggccgc cagccggtcc gatcaggagc
    11041 gccgcactgg accaaggaaa aactgttcac gctgtttcca aatctcgccg agatgcgcgg
    11101 ccgccacggc gggcgcatga gcggcggcga gcagcagatg ctgaccatcg cgcggaccct
    11161 gatgggaaac ccgtcgctgg tgctgctcga cgagccgtcg gagggattgt cgcccaagat
    11221 cgtggagcag atggtcgaag ccatcctgac catgaagaag gaaggcgtcg gcatcgtcgt
    11281 ctccgagcag aacctgcatt tcgcgagact gatttccgac cgcgcctaca tcatcgagcg
    11341 cggccgcatc tgcttcaacg gcaccatgcg cgagcttgac gcccgtccgg atatccgcga
    11401 cgcgcatctg gcgctgtgat cgccgggcgg aaacaacagg ggcggggtgc cgcagatgac
    11461 cagaagtgtt tccgccaaac ggaatgtaaa accgtcgcgg ccggcttacg tgctggagga
    11521 gcagatcgga ttcatcctgc gccaggtgtg gcagcgccac gccaccatct tcgcccgcga
    11581 gatcgggatc aatctgacgc cgacgcaatg ggcggcgctc gcaaaactca ccgagacagg
    11641 gccgtgctcg cagaaccagc tcggacggct gacggcgatg gacgtcgcca ccatcaaggg
    11701 cgtgatcgac cgcctcacgg cgcgcggcct gaccgagacc agccccgatc ccgaagacgg
    11761 tcgccgcctg ctcgtcagcc tgacgcgcgc gggtcagcag ctggccgaaa aagccgcgcc
    11821 gaacgcgctc gcgatcacca aggagacgct tgcgccgctc gatgccaggg agcgcgagac
    11881 gttggtcgcg ctattgagca agctgcggtg agcagtgtgc gtattcatac acttcggagt
    11941 agcgctttgt cgctgcccgg catagcatca agtagcacag acctgctttc gacgctcgtg
    12001 ctgacgtttc atattcctct ctaggagagg tcaaatggcg ttccgcatca aacggcggga
    12061 gttcatcggt ttgattggcg gcgcaacggt cgcatggccg ttcgttgcac tcgcgcagaa
    12121 gcctgaccgg tcgcggcgga tcggcgtcct catgtcgttg ggcgaaaacg atcccgaggg
    12181 aaaggctcag ctttccggat tcacgcaggg gcttgcggat ttgggttggt ccgacggtcc
    12241 aaacctgcga atggatgtgc gctggggcgg cggcgacgtc aacaagattc ggactttcgc
    12301 gaaagagttg gtggccgcgc agcccgatct gatcctctcg caagggacgc cggtgacggc
    12361 agcactccag cgggagacgc gcacgatccc gatcgtattt gtcgtcgtca ccgacccggt
    12421 cggcgagggg ttcgttgcgg gactgcccct tccgggcggg aatatcaccg gtttcatcac
    12481 gtcagaagcg gcgatgggca gcaagatgct ggacctgctc ctggatatcg cccccggcat
    12541 caaccgtgtc gcgatgatat tcaatcccga caccgctccc ggcggcggga agtattatct
    12601 cggcgatttc gaggcagctg cccgatcatc cagaattgcg ccaatcgcag cgcgtgctca
    12661 aagcgatgcc gaaatcgata ccgttgtgac gtcgcttgga cgagaacctg atggcggtct
    12721 cgttgtcatg ccggactttt tcatgctgaa ccatgtccag ccaatcttgt tgcaagcagc
    12781 gcgaagcaac gtacccacaa tctacccgtg gaggtatgtc gtgaccaagg aagccggtct
    12841 gatttcgtat ggacccgact tcagagatat tgttcgtcgc gccgctccct atgtcgatcg
    12901 cattcttcgc ggtgccaagc ccgccgattt accggttcaa gtgccggtga aattcgagat
    12961 ggctgtgaac gtcaagacag ccaaggcgct cggccttact gtgccaacgt cgcttctatt
    13021 gcgggccgac gaggtgatcg aataaagatg agcagcgccg ccgctgtggg agctggtcga
    13081 aagccggttc tcgggaaatt cggcgaacgg ggcctcgcct agaaagcgac ccccaaacgt
    13141 cgtccggcgc tccaaaccac ccggcaacgc ttcttggtgc caccgaagag caactcaaat
    13201 cgattgggca gcctgggctg cccaaagact tcgaggcagg cgccaccggg ggaatagtcg
    13261 accacagtgc agtcaataag ggggtctctc ggcccaacga tcagtttggc cgagctggaa
    13321 cccttgcctg ccggtcgaac gcgagcgaac cgtcgcggat acataccccg cccctggctg
    13381 tgacccagaa aatctgaccg ccagaatcac aggacatttt gaatacttcg tttagtttta
    13441 tagttatcga aacccaacga tgggtcgccg ggaagattct tatcaaaagc tcgacaatgc
    13501 cgaaatccag cccgcatccc gtgatcgcat ctgatcctgc tccagcccat cacccgaccg
    13561 cgaaatgcat catcgtgacg ccaccggaag cgtagttggg cacatcgccg gcaaccgcct
    13621 tgccttcctt cgaccccagc gcagccatca gcgctgcctc gttctcgaac tcggcttcga
    13681 agatgcaaaa ataaggcgcc ttgcctggtc ccagggactt cacatcgaac gaatatcggc
    13741 tcgccttgag gttcggtaat ttgctcacaa gcggtaaatg cgtctcgacg tagtatttcc
    13801 ggaaatgtgc gggatccttg ggatcattgt aaagaacgag catcttgtgc attaattgcc
    13861 tcccgatttc atctgacccg ccttcttgcc aaggccaagc tttggaacga agcggaccct
    13921 agcagacggt ctgggtgcgg gaaggcaaat gtcggtgacg gctgcgccca aggactgctg
    13981 gctgagtgcc aacagcggac tccttatcag gcaccaataa agggaaatgt cttcaggtct
    14041 tggggttgcc tgttctatca cgcgacaccg catcgatcag cagtggctga atgattggat
    14101 tgctgataag g
//
LOCUS       DATNOI010000314         7345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_543569,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000314 DATNOI010000000
VERSION     DATNOI010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543569"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VN968_12300"
     CDS             complement(<1..195)
                     /locus_tag="VN968_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30055.1"
                     /translation="MSKPSDSNALDHQGKPQAAAGVDRRRFFMMGGSAVAAAAVVPLA
                     SGEAEADESQAERVKARYKET"
     gene            complement(253..1038)
                     /locus_tag="VN968_12305"
     CDS             complement(253..1038)
                     /locus_tag="VN968_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HXL30056.1"
                     /translation="MRDAGLEQAIKAAGGVASLARAIGIAQPSVSAWSRIPAERVLAV
                     EALTQVQRFILRPDLYGPSEVQVSSKPEIDEIDALRAAEYGLLSLLLGKAPGADTLLR
                     VAALKGDASDLGTAHIELAAAAAATDDRAVSKEFFALFVGLGRGELLPYASYYMTGFL
                     HERPLARVREDLDVLGIERAGASREPEDHIATLLEVMAGLARGDFEADFAEQARFFER
                     HLRPWASRMFADLEMSRSANFYRAVGRVGRVFMELESEAFTLS"
     gene            complement(1153..1842)
                     /locus_tag="VN968_12310"
     CDS             complement(1153..1842)
                     /locus_tag="VN968_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HXL30057.1"
                     /translation="MSDEEQSADNKSFLARWSQRKHEAKQPDHDAPTVNSDAPSGPVA
                     ESDVVQEFDLSSLPKLEDMTATTDITAFLRKGVPEHLRNAALRKSWALDPAIRNYVNP
                     ALDYAYDWNTPGGVPGSSEIGTGMDVGRLVSQIMGGGSTVEPVVPAVSPGNEAASDPT
                     QSLEHDTPELELPAQAVRLSKETVSMAQKPANAEEGSHIVVGGAEHSEVSRSAAPQQV
                     VRRHGSAKPLV"
     gene            complement(1839..2357)
                     /locus_tag="VN968_12315"
     CDS             complement(1839..2357)
                     /locus_tag="VN968_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HXL30058.1"
                     /translation="MAMTESSRQVGVVLRRRSIDNPWIDHMWSPVMILDEVPATAPWT
                     VLSAEADATIYYAGAASIDLFSSDTANYRDNLADGEPRIWVALRRQDGGSELELTKVT
                     ADPTEGEAIFESGCDVIGTVPMPPDIASWIAAFVDEFHVERAFHKRQRDRADGNGQRM
                     PDGSPDERRGRI"
     gene            complement(2383..3402)
                     /locus_tag="VN968_12320"
     CDS             complement(2383..3402)
                     /locus_tag="VN968_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HXL30059.1"
                     /translation="MREFWVASGHHLTRRADHGGLVATPELIMAYLARPELMPPADAC
                     EAERNLHASVLADPLRPVSSADIAALADADARENWTFMMNFRDRLIAAPSLEAVYTNL
                     ARNGAGDLPPIFLSQLCHLILRNALERCDDPYTLRAAELFYRSQQAAVHDDALLLADT
                     EIVEAQQHAQHDMHLSPLAALLQPKPFGEIDVMDDENAWTYWSRSDAHAMVMNIGGNP
                     KARAGLCRVIERWVTHLLGVSVRVETVASIEDRDWRWFVGLDSEGTRIGNALWNGSVL
                     DDDAAGRIVALMRLNIEDPRLVDERVGTRPVYLILAMGADRVVRLKPQNLVAGLPLAS
                     SANDA"
     gene            complement(3399..3974)
                     /locus_tag="VN968_12325"
     CDS             complement(3399..3974)
                     /locus_tag="VN968_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6505 family protein"
                     /protein_id="HXL30060.1"
                     /translation="MLKFPRTIRLDPSDTFVFERAAVPGEWAVSGAFVFWNQDPAMLG
                     QKQRVALRSGFLGIDSLGWSTLAIVTEATEADRHAIVERLAGQLLEKFGAPDLEAARL
                     AAEEEVAFAASLCNHPPQTLLAVQRSVEDGEIRERFRTLKPREAAPGADRLHAHASAF
                     TFHEVEGDIEPAEEADLLGMIKTDRMTTDRR"
     gene            complement(3982..4737)
                     /locus_tag="VN968_12330"
     CDS             complement(3982..4737)
                     /locus_tag="VN968_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate--protein ligase family protein"
                     /protein_id="HXL30061.1"
                     /translation="MASRLAGMEQTLDLPPGYTLVALREYGDAFAHGCDIAEQAGAGT
                     LVWVRRYDLVEFAVVLEPDEPLVSARRALFAGMNAAADAIAAHCPPEREVSFDWPDTI
                     RFDGGLLGGARLGWPTDCAETSVPEWLVLGFILRAADMAHVEEVQAAAGVSLLSEGFE
                     MIETDAIIGSFARHLMTAFDRWNEHGFEAIARDYLARLVKRKAGERRTIDLNGDLLVG
                     PPSGSGPADRMRLVDALARVVWYDPHARGPKTG"
     gene            4914..6950
                     /locus_tag="VN968_12335"
     CDS             4914..6950
                     /locus_tag="VN968_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXL30062.1"
                     /translation="MSAASSHLLICSCEKTMPLDAQAIGRGCAARITQANQLCGLELE
                     RFKQALADGAPITVACTQEAPLFREVAENFPQAQLTFVNIRENGGWSNDAAAAGPKAA
                     ALIAAADEDMPPISLVTLESNGVALIYGRDEVAIEAAQRLADRLDITVLLTRPGDVTP
                     RRTNEFPVVKGTIRNARGHLGRFELDIDDYALPLPSSRAKLVFGPSRDGATSSCELIL
                     DLSGGVPMFPAHDLRPGYLRADPRDRAAVERAIADAGGLVGTFDKPRYIHFEESLCAH
                     SRSGITGCTRCLDLCPTGAITPNGNSVVIDVNVCAGCGSCASVCPTGAASYSLPSADA
                     LMRRLRALLQTYRRAGGSNAVVLFHDGEHGDDMIDALARFGAGLPANVLPLRLNEVTQ
                     LGAENIAAVFAYGGAGVAVLARARPRHDILGLRRAAEMSDAIISALGFGAGLVRIIET
                     DDPDQLRVVLDAAPAGIATQKPAGFIPRGAKRSVLETTFRELHLAAPLPVDVVPLAAG
                     APFGGVKLNVEGCTLCHACVTACPTGALSDNPDRAMLRFTESLCVQCGLCQSTCPEKV
                     ITLEPRLDFQAWNAPLRVLKEEEPFLCIACGKPFGTKSSIDRVLAKLGEKHWMFQGAN
                     AKRIDVIKMCADCRVEAVVNEGFDPHAASQRPPVMTSEDYLRARDAKKDDPLGS"
     gene            6961..>7345
                     /locus_tag="VN968_12340"
     CDS             6961..>7345
                     /locus_tag="VN968_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HXL30063.1"
                     /translation="MSAIHPDLKARATSKAAGIPGVKHVIAVASGKGGVGKSTTSCNL
                     ALGFAAIGLKAGILDADIYGPSQPKLFGLRGKPRQVGPRMLEPMERFGVKVMSIGFLV
                     EEDLAMIWRGPMVISAITQMLREVAW"
BASE COUNT         1314 a         2416 c         2268 g         1347 t
ORIGIN      
        1 ggtttccttg tagcgcgcct tgacgcgctc ggcctgcgac tcgtccgctt cggcctcgcc
       61 gctcgccagc ggcacgaccg cagccgcagc taccgccgat ccacccatca tgaagaagcg
      121 gcgccggtcc acgcctgccg cagcctgcgg cttgccttgg tggtcgagcg cgttactgtc
      181 ggatggcttg ctcattgcga tctcctcgaa gtcatctttt ccaagtcaat cttttggctg
      241 ccttcggcgt catcaggaca gcgtaaaggc ctctgattcc agttccataa agacacgccc
      301 gacacggccg acggcacggt agaaattggc tgaccgggac atctcaagat cggcgaacat
      361 ccgcgaagcc cacggcctga gatgccgctc gaagaaacgc gcctgctcgg cgaagtcggc
      421 ttcgaagtcg ccgcgcgcaa ggccggccat cacctcaagc agggtcgcga tgtggtcttc
      481 cggctcccgc gacgcgcctg cgcgttcgat tccgagcaca tccagatctt cgcggacacg
      541 cgccagcggg cgctcgtgaa gaaagccggt catgtaatag gacgcatagg gaagcagctc
      601 gccgcgacca agtccgacga acagagcgaa gaactccttg ctgacagcgc gatcatccgt
      661 cgcggcagcc gccgccgcga gttcgatatg cgccgtgcca agatcggacg catcgccctt
      721 caacgcggca acccgcaaca gcgtatccgc gccgggcgcc ttgccaagca gcagggacag
      781 caagccgtat tccgccgcgc gcagcgcatc gatctcgtcg atttcgggtt tcgatgacac
      841 ctgaacctcg gacggtccgt aaagatcggg gcgcaggatg aaacgctgaa cctgcgtcag
      901 cgcctcgacg gccagcactc gttctgccgg aatgcgcgac catgccgaca ccgaaggctg
      961 ggcaatgccg atcgcccggg ctagcgacgc gaccccacct gctgctttga tggcctgttc
     1021 cagaccggcg tcacgcatgg cccgaccgac ttctgcccca cccgcggctc ttcatcgggt
     1081 atctttttag aattagaatt gtcccaaagt gtttgtaaca taggctctgc ctatgtcctc
     1141 aaaaactttt gtctaaacca gaggtttagc tgagccatgg cgtcgcacca cctgctgcgg
     1201 cgcagcagag cggctcactt cggaatgctc cgcgcccccg accacaatgt ggctgccttc
     1261 ttcggcattc gcgggctttt gcgccatcga aaccgtttct ttgctcaatc tcacggcctg
     1321 agctggcaat tcaagctccg gcgtatcatg ctcaagggat tgcgtgggat cgctggctgc
     1381 ctcatttccg ggggagacgg ccggtaccac aggttcgacc gtcgatccgc cgcccatgat
     1441 ctgcgaaacc agccgcccga catccatgcc agttccgatc tcgctgctgc ccggtacgcc
     1501 gcccggcgta ttccagtcgt aggcgtagtc gagcgcagga ttgacgtaat tgcggatcgc
     1561 gggatccagc gcccaggatt ttcgcaacgc cgcattgcgc agatgttcgg ggacaccctt
     1621 gcggaggaag gcggtgatat cggtcgttgc cgtcatgtcc tcaagcttcg gcaggctgga
     1681 gagatcgaac tcctgcacga catcgctttc ggcaaccggc ccggacggcg catcggagtt
     1741 gacggtcggg gcgtcgtgat ccggctgttt cgcctcgtgc ttgcgctgcg accatcgcgc
     1801 cagaaaactc ttgttgtccg cgctttgctc ctcgtcgctc atatgcggcc tcttcgttca
     1861 tccggagaac catcgggcat tcgctggccg ttgccgtccg cacgatcccg ctggcgctta
     1921 tggaatgcgc gctcgacatg gaattcatcg acgaaggcgg cgatccacga cgcgatgtcg
     1981 ggcggcatcg ggacggtacc gatcacatcg cagccgctct cgaatatcgc ctccccttcg
     2041 gtcggatcgg ccgttacttt tgtcagctcg agttcggagc cgccatcctg acgccgcagc
     2101 gcgacccaga tgcgcggctc gccgtcggcg agattgtcgc gataattcgc ggtgtccgag
     2161 ctgaacaggt cgatgcttgc ggcgcccgcg tagtaaatcg tcgcgtcggc ctccgcggat
     2221 aacacggtcc atggcgcagt cgccggcact tcatcgagaa tcatcaccgg cgaccacata
     2281 tgatcgatcc atggattgtc gatcgaacgg cggcgcagga ccacaccgac ttggcgggat
     2341 gactcggtca ttgccatggt tgatcctccg ttcgccggcc gatcaggcgt catttgctga
     2401 tgacgcgagc ggcagccccg cgaccagatt ttgcggcttc aggcgcacca ccctgtccgc
     2461 gcccatcgcc agaatgagat agaccggcct ggtgccgacg cgttcatcca ccagtcgcgg
     2521 atcctcaatg ttcaaacgca tcagcgcgac gatgcgcccc gccgcgtcgt catcgagcac
     2581 ggagccgttc cacaaagcgt tgccgatccg ggtgccctcg ctgtcgaggc cgacgaacca
     2641 gcgccagtct cggtcttcga tcgaagcaac cgtttccacc ctgacggaaa cgccgagcag
     2701 atgggtgacc catcgctcga tcacgcggca cagcccggca cgcgccttcg ggttgccacc
     2761 gatgttcatg accatcgcat gggcgtcgga gcgcgaccag taagtccagg cgttttcgtc
     2821 gtccatgaca tcgatctcgc cgaacggctt cggctgcagc aaggctgcga gcggggacaa
     2881 gtgcatgtcg tgttgcgcat gttgctgcgc ctcgacgatc tcggtatcgg ccagcagaag
     2941 cgcatcgtcg tggaccgccg cctgctggct gcgatagaac agttcggccg cgcgcaacgt
     3001 gtagggatcg tcgcatctct caagcgcgtt gcgcaggatc agatggcaga gctgcgacag
     3061 aaagatcggc ggcagatcgc cggcgccgtt gcgggccagg ttggtataaa cggcttcaag
     3121 cgagggcgcg gcgatcagcc ggtcgcggaa gttcatcatg aaagtccagt tctcgcgcgc
     3181 atcggcgtcg gcgagagcgg caatgtccgc acttgagacc gggcgcaggg gatcggccag
     3241 cacgcttgca tgcagattgc gttcggcctc gcatgcatcg gccggcggca tcaattcggg
     3301 ccgcgccaga taggccatga tcagttcggg tgtcgcaacc agcccgccat gatcggcgcg
     3361 gcgcgtgagg tgatggcccg aggcgaccca gaattctctc atctgcgatc cgtcgtcatg
     3421 cggtcagtct tgatcattcc gagaagatcg gcctcctcgg cgggctcgat gtcgccctcg
     3481 acctcgtgaa aggtgaaagc gctggcatgg gcgtgcaacc gatccgcgcc cggtgctgct
     3541 tcgcgcggct tgagcgtgcg aaagcgctcg cggatttcgc cgtcctcgac gcttcgctgc
     3601 acggcgagca acgtctgagg cgggtgattg cacaacgagg cggcaaacgc gacctcttcc
     3661 tcggcggcaa ggcgcgcggc ctcaaggtcg ggtgcgccga acttctccag caattggccc
     3721 gcgaggcgct cgacgatggc atgcctgtcc gcttccgtgg cctcggtgac gatcgcaagc
     3781 gtggaccagc ccagactgtc gataccaaga aagcccgagc gcaacgcgac gcgctgtttc
     3841 tggccgagca tggccggatc ctggttccag aatacaaagg cgccggaaac agcccattcg
     3901 ccgggtacgg ccgcccgctc gaacacgaat gtgtccgacg ggtcgagcct gatcgtccgc
     3961 ggaaatttca gcatgcccgc ctcatccggt tttcggaccg cgcgcgtgcg gatcatacca
     4021 aactaccctg gccagcgcat ccacaagacg catgcggtcg gccggcccgc tgccggatgg
     4081 cgggccgacc aggaggtcgc cattgaggtc gatcgttcgg cgctcgcctg ccttgcgctt
     4141 cacaagccgt gccagatagt cccgcgcgat cgcctcgaac ccgtgctcgt tccaccggtc
     4201 gaacgcggtc atgaggtgac gggcgaagct gccgatgatc gcgtcggtct cgatcatctc
     4261 aaaaccctcg ctcaacagcg aaacgccggc ggcggcctgc acttcctcga catgggccat
     4321 gtccgccgcg cgcagaatga aacccagcac cagccattca ggcaccgagg tctcggcgca
     4381 gtccgtcggc cagccgagcc gcgcaccgcc cagcaagccc ccgtcgaacc tgatcgtgtc
     4441 aggccagtcg aagctcacct cccgttcggg cgggcaatgg gcggcaatcg cgtccgccgc
     4501 cgcgttcatg ccggcaaaca gcgcccggcg tgccgacacc aaaggctcgt cgggttcaag
     4561 caccacggcg aattcgacca ggtcgtagcg tcgcacccag accagcgtcc cggcccccgc
     4621 ctgctccgcg atatcgcagc cgtgggcgaa cgcgtcgcca tattcgcgca gcgctaccag
     4681 ggtgtagccg gggggcagat cgagtgtctg ctccatcccg gcaagacgcg acgccatcaa
     4741 acgtgtcatc cttaattgtt tgcaatccgc cactatatgt tatcccgata acggtggtaa
     4801 cgcgagcggg tctgggcctt gcggatcttc ccgtcagcag gtaccgcgga accgcaggat
     4861 gaagccaccg cgcggccggc gcgccggttc agggtaaatt caatgagcgc ggtatgagtg
     4921 cggcatcatc ccatctcctg atctgcagct gcgagaagac catgccgctc gatgcgcagg
     4981 cgatcggtcg cggctgcgcg gcaaggatca cgcaggccaa ccagctctgc ggcctcgagc
     5041 ttgagcgctt caagcaagcc cttgccgacg gcgcgcccat cacggtcgcg tgcacccagg
     5101 aagcgccgct gttcagggag gtcgcggaga atttcccgca agcgcagctt accttcgtca
     5161 acattcgcga gaacgggggc tggtcgaacg atgcagccgc ggctggtccg aaagccgcgg
     5221 cgctgattgc cgcagcagac gaggacatgc caccgatttc actggtgacg ctcgagagca
     5281 acggcgtcgc gctgatctat ggccgcgacg aggttgcaat cgaggccgcc cagcgtctcg
     5341 cggaccgtct cgacatcacc gtgcttctca ccaggccagg cgacgtgacg ccacggcgta
     5401 ccaacgagtt tcctgtcgtc aagggcacga tccgcaacgc ccgcggacat ctcggccggt
     5461 tcgagcttga tatcgacgac tacgcgctgc cgctgccatc ctcgcgcgcg aagctggtgt
     5521 tcgggccctc gcgcgatggc gcaacctcga gctgcgagct gatccttgat ctcagcggcg
     5581 gggttccgat gttccccgcg catgatttgc gtccgggata tctgcgcgcc gatccgcgcg
     5641 accgggccgc tgtcgaacgc gcgattgcag atgcgggcgg tctggtgggc acgttcgaca
     5701 agccgcgcta tattcacttt gaagagtcgc tctgcgccca ttcgcgctcc ggcatcaccg
     5761 gatgcacgcg ctgtctcgac ctgtgtccga ccggcgcgat cacgccaaat ggcaattccg
     5821 tcgtgatcga cgtcaatgtg tgcgcgggct gcggttcatg tgcttcggtg tgtccgaccg
     5881 gggcggcatc gtattcgctg ccgagcgcgg acgcgctgat gcggcggttg cgcgcgctat
     5941 tgcagaccta tcgcagggca ggcggcagca acgctgttgt gttgtttcac gacggcgagc
     6001 acggcgacga catgatcgac gccctggcgc ggttcggcgc cggtttgccg gcgaatgtgc
     6061 ttcctctccg cctcaacgag gtgacgcagc tcggagccga gaacatcgct gccgtgtttg
     6121 cgtatggcgg cgccggcgtc gccgtattgg cgcgcgccag gcccaggcac gacattctcg
     6181 gacttcgtcg agcagccgag atgtccgacg ccattatttc agcgctcggc ttcggcgcag
     6241 gccttgtgcg catcatcgag accgacgatc cggaccaact ccgcgtcgtc ctcgatgcag
     6301 cgcccgctgg catcgcgacg caaaagccgg ccggctttat tccacgcggc gcaaaacgta
     6361 gtgtgcttga gaccacgttc cgtgaactcc acctcgctgc ccccctcccc gtcgatgtgg
     6421 tgccgctggc tgccggcgca ccgttcggcg gcgtgaagct gaacgttgaa ggctgtacgc
     6481 tctgccatgc ctgcgtcacc gcctgcccga caggagccct atcggataat cccgaccgcg
     6541 cgatgctgcg ttttaccgaa agcctctgcg tgcaatgcgg gctttgccaa tccacctgtc
     6601 ccgagaaggt catcacgctg gagccgcgtc tggatttcca ggcgtggaac gcgccgctgc
     6661 gggtgctgaa ggaagaagag ccattcctgt gtatcgcctg cggcaaaccg tttggaacca
     6721 aaagctcgat cgaccgcgtg ctggccaagc tcggcgagaa gcactggatg ttccagggtg
     6781 ccaatgccaa acgtatcgat gtcatcaaaa tgtgcgcgga ttgccgcgtc gaagccgtgg
     6841 tgaacgaggg tttcgacccg catgcggcat cgcaacgccc acccgtcatg acatcagagg
     6901 attatttgcg cgcgcgcgat gcaaagaagg acgatccgct cggatcgtga ggaacccgcc
     6961 gtgtccgcca tccatccaga tctgaaagca cgcgcgacct cgaaagcggc cggtattccc
     7021 ggcgtaaaac acgtcatcgc agtcgcctca ggcaagggag gcgtcggcaa atccaccacc
     7081 tcatgcaatc tcgcgcttgg ctttgcagcc attggtctga aagccggcat cctcgatgcc
     7141 gacatctatg gtccgtcgca gccgaaactg ttcggtctgc gcggcaaacc gcgccaggtg
     7201 ggcccgcgca tgctcgagcc gatggagcgc tttggcgtca aggtgatgtc gatcggcttc
     7261 ctggtggagg aagacctcgc catgatctgg cgcgggccga tggtgatctc ggccattacg
     7321 caaatgctgc gcgaagtggc gtggg
//
LOCUS       DATNOI010000315         7308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_738705,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000315 DATNOI010000000
VERSION     DATNOI010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_738705"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1973
                     /locus_tag="VN968_12345"
     CDS             <1..1973
                     /locus_tag="VN968_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HXL30064.1"
                     /translation="ARMSLVLIAFAGFALIVYLSTHGFDRAVLLIPTWFLLLVWVIAA
                     GMTVGGSVTNDIVGPALLGGLVLIVMLIGFTVMQHAFAGGGATTGMVSDVERRALALT
                     GSGDLIWDWDVSADKVFTSPETESLLGLKRGTLEGPAAKWLEVLHPLDQDRFRAALDS
                     VLDQRRGRLVQDFRLRTPDGHFMWFALKARPVVGSDGEVSRVVGTLTDVTEGKNAEER
                     LLHDSVHDNLTGLPNRKLFIDRLGAVANFTRTMPNLRPTLMVIDLDRFKQVNDSVGIA
                     VGDSILLTLARRLTRILKPQDTLARLSGDQFGLILISEQDPPRITAFAETIRKTIRAP
                     IAFNDREIFLTASIGLALSDPQTQLTEEIIKDAELAMYHSKRIGGDRIDVYKPAMRAR
                     KTDRLTLESELRRAIERGEITILYQPIVRLEDRSIAGFEALARWDHPKLGRMSPSEFI
                     AIAEEIGLIVDLGMFVLDQTARQLSIWQRAMRSREPIFASVNVSSRQLLRHDLIHDIR
                     TVLSRSSVARGTLKLELTESLVMENPEHAAQMLTRIRELGTGLSLDDFGTGHSSLAYL
                     QRFPFDTIKIDQSFVRTSSRGTRPVILKSIIAMAHDLGMDVVAEGAETDSDAVELYQL
                     GCEYAQGFAFGEPMDADAAMRLLTEERLEAAS"
     gene            complement(2151..2399)
                     /locus_tag="VN968_12350"
     CDS             complement(2151..2399)
                     /locus_tag="VN968_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30065.1"
                     /translation="MNDYDLLRDYLKKQTLPEFVLSFEKIEEVIDAALPRAAHRASWW
                     ETLRSPQEKMPQREACLAGGYIATRLADGKSVRFKRMK"
     gene            complement(2544..3425)
                     /gene="galU"
                     /locus_tag="VN968_12355"
     CDS             complement(2544..3425)
                     /gene="galU"
                     /locus_tag="VN968_12355"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HXL30066.1"
                     /translation="MKIRKAVFPVAGLGTRVLPATKAMPKEMLTIVDKPLIQYVVDEA
                     KEAGIEHFIFVTGRNKAVIEDHFDRMYELDATLAARGKKAEMEILARDQPEAGAMSFT
                     RQQAPHGLGHAVWCARDIVGNEPFAVVLPDELVLNTPGCLKQMIDAAGKLGDKSNLIA
                     VEEVPAHLTHQYGICGVGKRHKHASMFEIDGMVEKPPKGTAPSNLSITGRYILQPEIF
                     KILETQERGAGGEIQLTDAMIGLAKTQSFYGVEFEGERHDCGSKAGFLRANIAFGMAR
                     ADLRDDLRAEMKKYLEE"
     gene            3857..5284
                     /locus_tag="VN968_12360"
     CDS             3857..5284
                     /locus_tag="VN968_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HXL30067.1"
                     /translation="MTRRTGPSAKMRTHAVIMAVALLLAGEARAQSSGGGFLNFFDNI
                     FPGSVPKAGQTAPSGQPPGTSAPAQTAPAPGGAAPPWSGEDGASGHPLMTAGAIREAA
                     ANFSTCVASMWPDAARRNISQESFQRFTAGLEPDLRIMDLMDSQPEFTKAIWDYLDIL
                     VNDDRLAKGREVLARYKPQFDAAEKTYGVDRYSIAAIWGIESNYSTQMGDRSVLQSTA
                     TLACIGRRQKYFRDEFLSALEILHHGDLRPEQMRGSWAGAFGPTQFMPTAFKRYAVDA
                     DGDGRRDVVDDAADLIASTANNLKKDGWQPGQSWGFEVVMPKGFDYMLADRAKAMTMA
                     QWEHLGVTRAGGQPFPRVADKAYLLAPAGAGGPGFLMLQNFRVIMKYNPAEAYALAIG
                     HFADRLRGGAPFVQPWPRQERVLSRAERLELQQLLAQRGFYQGTPDGQLGGQTREAVR
                     GFQASIGAPADGFASSDVLERLRGR"
     gene            5443..7086
                     /locus_tag="VN968_12365"
     CDS             5443..7086
                     /locus_tag="VN968_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HXL30068.1"
                     /translation="MPKPKSFFRVFTETGPLIVLAVAVALLVGIVGPASAQFFNFGGP
                     PQQRSGGWFGGDLFAPFQQPAPKRVIREDFSKAPAPEKRDNVPERNVLVLGDAMADWL
                     AYGLEGAYAEQPDMGVIRRHKTLSGLIRYQPKGDPADWPAAAKGILATEKPDAIVVML
                     GLNDRVSMREPVVEKTDSKSTDKKNDKKDAKAKPDAKPADAKPTAKPDDAGNAAAKPD
                     DKPVDAELSPDDAADSDAPPVIAPEKSTRSPNGVYEFREERWVELYTKKIEEMIAVMK
                     SKGVPVLWVGLPAVRGTKATADMLFLDSLYRDAAGKAGITYVDVWDGFVDEAGRFMQQ
                     GPDFEGQPRRLRAYDGVFFTPAGARKLAHYVEREITRLMAARSAPIELPTEPATPDAN
                     ALPGQPAPRPLAGPIVPLVASSVGTDQLLGGPGSRPAAVDALAARTLVKGEPLAPPAG
                     RADDFVWPRREVGREQAKGETPVASASPDGTIAAPGAAPKPQQRRLRPAQSGPPAMRE
                     FFGFGNAPRQPPPPRAAPGVLRQPGTIGRSAWVPDFFTR"
     gene            complement(7125..>7308)
                     /locus_tag="VN968_12370"
     CDS             complement(7125..>7308)
                     /locus_tag="VN968_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30069.1"
                     /translation="AKDAYENSGDTFREGSEVVAKKVQDNPLGALLVAGGIGFALAML
                     MTRPPRRPPQRWRYYS"
BASE COUNT         1261 a         2407 c         2346 g         1294 t
ORIGIN      
        1 tcgcgcggat gtcgctggtg ctgatcgcgt tcgcgggctt cgcgctgatt gtctatctgt
       61 cgacccatgg cttcgaccgc gcggtgctgc tgatcccgac ctggttcctg ctgctggtct
      121 gggtgatcgc ggccggcatg acggtcggcg gcagcgtcac caacgacatc gttggcccgg
      181 cgctgctcgg cggcctcgtg ctgatcgtga tgctgatcgg atttacggtg atgcagcacg
      241 ccttcgccgg cggcggcgcc accaccggca tggtctccga tgtcgagcgc cgggcgctgg
      301 cgctgaccgg ctcgggtgac ctgatctggg actgggacgt ctcggccgac aaggtcttca
      361 ccagccccga gaccgaaagc ctgctcggcc tgaaacgagg cacgctggaa ggccctgccg
      421 cgaaatggct cgaggtgctg catccgctcg atcaggatcg tttccgcgcc gccctcgaca
      481 gcgtgctcga ccagcgccgc ggccggctgg tgcaggattt ccggctgcgc acgcccgatg
      541 gccacttcat gtggttcgcg ctgaaggcac gtccggtggt cggctccgat ggcgaagtct
      601 cgcgcgtggt cggcacgctc accgacgtca ccgaaggcaa gaacgccgaa gaacgcctgc
      661 tgcacgattc ggtgcacgac aatctcactg gtttgccgaa ccgcaaactg ttcatcgacc
      721 gcctcggcgc ggtcgccaat ttcacccgga cgatgccgaa cctgcggccg acgctgatgg
      781 tgatcgacct cgaccgcttc aagcaggtca acgattccgt cggcatcgcg gtcggcgatt
      841 cgatcctgct gacgctggcg cggcggctga cgcgcatcct caagccgcag gacacgctgg
      901 cccggctgtc cggcgaccag ttcgggctga tcctgatatc ggaacaggat ccgccgcgca
      961 ttaccgcctt cgccgagacc atccgcaaga ccatccgggc gccgatcgcc ttcaacgacc
     1021 gcgagatttt cctgactgcc tcgatcggcc ttgcgctcag cgatccgcag acgcagctga
     1081 ccgaagagat catcaaggac gccgagcttg cgatgtatca ttccaagcgc atcggcggcg
     1141 accgcatcga cgtctacaag ccggcgatgc gcgcgcgcaa gaccgaccgc ctgacgctgg
     1201 aatccgagct gcgccgcgcc atcgagcgcg gcgaaatcac catcctgtac cagccgatcg
     1261 tgcggctgga ggatcgctcg attgcgggct tcgaggcgct ggcgcgctgg gatcacccaa
     1321 aactgggacg gatgtcgcct tcggaattca tcgcgattgc cgaggagatc ggcctgatcg
     1381 tcgatctcgg catgttcgtg ctggaccaga ccgcgcggca gctttcgatc tggcaacgcg
     1441 cgatgcgttc gcgtgagccg atcttcgcca gcgtcaacgt ctcctcgcgg caattgctgc
     1501 ggcacgatct gatccacgac atccgcaccg tactgtcgcg ctcctcggtg gcgcgcggca
     1561 cgctgaagct cgagctgacg gaatcgctgg tcatggagaa cccggaacat gcagcgcaga
     1621 tgctgacgcg gatccgcgaa ctcggcaccg gactgtcgct ggacgatttc ggcaccggcc
     1681 attcgtcgct ggcctatctg cagcgctttc cgttcgacac catcaagatc gaccagtcgt
     1741 tcgtgcgcac ctccagccgc ggcacgcggc cggtgatcct caaatctatc atcgccatgg
     1801 cccacgacct cggcatggac gtggtggccg aaggcgcgga aaccgattcc gacgcggtgg
     1861 agctttatca gctgggctgc gaatacgccc aaggctttgc cttcggcgag ccgatggacg
     1921 ccgacgccgc gatgcggcta ctgaccgagg aacggctcga ggccgcaagc tgacccttcg
     1981 tcatggccgg gcaaaagcgc caacgggtcc gcgcaaagcg cggcccgatg acaggctccg
     2041 cgcgtcttcg cgctagatga cccggcaagc catctattca gaaaatgttt cggcgaagtt
     2101 tgatggatac gcgggtcgag cccgcgtatg acgtacgcgt gttacattcc ctacttcatc
     2161 ctcttgaacc gcacgctctt gccgtccgcg agccttgtcg cgatgtagcc gccggcaagg
     2221 caggcctcgc gctgcggcat cttttcctgc gggctgcgca acgtctccca ccatgacgcc
     2281 cgatgcgccg cgcgcggcag tgccgcgtcg atgacctcct cgatcttctc gaagctcagc
     2341 acgaattcgg gcagcgtctg cttcttcagg taatcgcgca gcaggtcgta atcgttcacg
     2401 gcgcagcccg gtaggtcgtc atgctcgcga aggcgggcat ccagtacgcc gtgccgcccg
     2461 tgattgatca ccggcgccgc ggaatactgg atcatccgct ttcgcggatg atgacggatg
     2521 agtgtgaacg cgcgccatcg agctcactcc tccagatact tcttcatctc cgcgcgcaaa
     2581 tcgtcgcgca gatcggcgcg cgccatgccg aaggcgatat tggcgcggag gaaaccggcc
     2641 ttggagccgc aatcgtgccg ctcgccctcg aattcgacgc cgtagaagct ctgggttttg
     2701 gcgagcccga tcatcgcatc ggtgagctgg atctcgccgc cggcgccgcg ctcctgggtt
     2761 tccaggatct tgaaaatttc cggctgcagg atgtagcgcc cggtgatcga cagattagac
     2821 ggcgcggtgc ccttgggcgg cttctccacc atgccgtcga tctcgaacat gctggcatgt
     2881 ttgtggcgct tgccgacgcc gcagatgcca tactgatggg tgagatgcgc cggcacttct
     2941 tccaccgcga tcaggttgga tttgtcgccg agcttgccgg cggcgtcgat catctgcttg
     3001 agacagcccg gcgtgttcag caccagttcg tccggcagca ccaccgcgaa cggctcgttg
     3061 ccgacgatgt cgcgggcgca ccacaccgcg tggccgaggc cgtgcggcgc ctgctgccgc
     3121 gtgaaactca tggcgcccgc ctcgggctga tcgcgcgcca aaatctccat ctcggccttc
     3181 ttgccgcgcg cggcaagcgt ggcgtcgagc tcgtacatcc ggtcgaaatg atcctcgatg
     3241 acggccttgt tgcggccggt gacgaagatg aaatgctcga tgccggcctc cttggcctcg
     3301 tcaaccacat actggatcag tggtttgtcg acgatggtga gcatttcctt cggcatcgcc
     3361 ttggtggcgg gcaggacgcg ggtgccgagg ccggcgacag gaaaaacggc tttgcggatt
     3421 ttcatgcggt atcgggatgc catcaagaga gcgtgcggaa aaagagagtg cggaaagcga
     3481 ggttcgcacg ttggtagccg gtttgcatcc gcgaacaaag gctgatgtgt cgagcgcgaa
     3541 acttcgtcaa gcgtacagta aattggcaga tgtcccctcc ccatcccggg cctgcctggg
     3601 ttgtgttttt atttttgcgc aagtcgcgca ggccccggtt agggagggga tgaacaaaaa
     3661 atctcggact ggcgttaccc ccttctccaa ctctccccca caaggggcag agagcaggtc
     3721 ttgcgtgtgg cgccgctgat gttccttgcg tccgaaggca agattcccga caacaaaatc
     3781 tcgctttgtt aagctattgg aaaccctgac aaagcctcct gaaggcggga tatggagact
     3841 agcccgggtg agagacatga cgcgacggac cggaccatcg gcgaaaatgc gaacccacgc
     3901 ggtcatcatg gcggtggcgc tgctgcttgc cggcgaggca cgcgcgcagt cttccggcgg
     3961 cgggttcctg aatttcttcg acaacatttt tccgggctcg gtcccgaaag ccggccagac
     4021 cgctccgtcc ggacagccgc ccggcacttc ggcgccagca caaaccgcgc ccgcccccgg
     4081 cggcgcggca ccgccctgga gcggcgagga cggcgcctcc ggccatccct tgatgaccgc
     4141 gggcgcgatc cgcgaggcgg ccgccaattt cagcacttgc gtcgcctcga tgtggcctga
     4201 tgccgcacgc cgcaacatct cgcaggagag ttttcagcgc ttcaccgcgg ggcttgagcc
     4261 ggacttgcgc attatggacc tgatggattc gcaacccgaa ttcaccaagg cgatctggga
     4321 ttatctcgac atcctggtca acgacgatcg cctcgccaag ggccgcgagg tcctggccag
     4381 gtacaaaccg caattcgacg ctgcggaaaa aacctatggc gtcgaccgtt acagcatcgc
     4441 ggcgatctgg gggatcgaat cgaattattc cacccagatg ggcgaccgca gcgtgctgca
     4501 gtcgaccgcg acgctcgcct gcatcggccg ccgccagaaa tatttcaggg atgagttcct
     4561 ctcggcgctg gaaatcctgc accatggcga tctgcgcccg gagcagatgc gcggctcctg
     4621 ggccggcgcg tttggcccga cgcagttcat gccgaccgcc ttcaagcgct acgcggtgga
     4681 tgccgacggc gacggccgcc gcgacgtcgt tgacgacgcc gccgacctga tcgcatcgac
     4741 cgccaacaat ttgaagaaag acggttggca gcccggccag agctggggct ttgaggtcgt
     4801 gatgcccaag ggcttcgact acatgctggc ggatcgcgcc aaggcgatga cgatggcgca
     4861 atgggagcat ctcggcgtca cgcgcgccgg tggccagccc ttccctcgtg tcgccgacaa
     4921 ggcctatctg ctggcgcccg ccggcgccgg ggggccgggc ttcctgatgc tgcagaattt
     4981 ccgggtgatc atgaaataca acccggcgga agcctatgcg ctggcgatcg gccatttcgc
     5041 cgaccgcttg cgcggcggcg cgccgttcgt gcagccctgg ccccggcagg agcgggtgtt
     5101 gtcgcgcgcc gagcggctgg aactgcagca gcttctggcc cagcgcggct tctatcaggg
     5161 tacccccgat ggccagctcg gcggacagac ccgggaggcg gtgcgcggct tccaagcctc
     5221 catcggggcg ccggcggacg gatttgcctc ctccgacgtg ctggagcggc tcagggggcg
     5281 gtgaaacacc ggttccattg ccgaaagtaa ccttgaggcg gtcatttgcg cagcgccctt
     5341 gacggcggcg gcggatcgcc gatttatggg aggaatctgc ccgcttgcgg cctgattccg
     5401 ctattatgac cattgccctg aattccattg cgatcgatgc gcatgccgaa gccgaaatcc
     5461 tttttccggg tgtttaccga gaccggcccg ctgatcgtgc tggccgtcgc ggtggcgctg
     5521 ctggttggga tcgtcggacc ggcatccgcg cagtttttca attttggcgg accgccgcag
     5581 cagcgtagcg gcggctggtt cggtggcgat ctgttcgcgc ccttccagca gccggcgccc
     5641 aagcgggtta tccgcgagga tttttccaag gcacccgcgc cggaaaaacg cgacaacgta
     5701 ccggagcgca acgtgctggt gctcggcgat gccatggcgg actggctcgc ctatggtctc
     5761 gagggcgcct atgccgaaca gcccgacatg ggtgtgatcc ggcggcacaa gaccctctcc
     5821 ggcctcatcc ggtaccagcc caagggcgat ccggccgact ggcccgcggc ggccaaaggc
     5881 atcctcgcca ccgagaagcc cgacgccatc gtggtcatgc tcggcctcaa cgaccgggta
     5941 tcgatgcgcg agccggtcgt cgagaaaacc gattccaaat cgaccgacaa gaagaacgac
     6001 aagaaagacg ccaaagccaa accggacgcc aagcctgcgg atgcaaaacc cacggcgaag
     6061 ccggatgacg ccggtaatgc cgccgcgaag ccggatgaca aaccagtcga cgccgaattg
     6121 tctccggacg atgccgccga tagtgatgcg cccccggtca tcgcgccgga gaagagcaca
     6181 cgctcgccga acggcgtcta tgaatttcgc gaggaacgct gggtcgagct ctacaccaag
     6241 aaaatcgaag agatgatcgc ggtgatgaaa tccaagggcg tgccggtgct gtgggtcggc
     6301 ctgcccgcgg tgcgcggcac caaggcgacg gcggacatgc tgttcctgga ttcgctgtac
     6361 cgtgacgccg ccggcaaggc cggcatcacc tatgtcgatg tgtgggatgg atttgtcgac
     6421 gaggccggcc gcttcatgca gcagggcccg gacttcgaag gccagccccg ccggctgcgc
     6481 gcctatgacg gcgtgttctt caccccggcc ggcgcgcgca agctcgcgca ttacgtcgag
     6541 cgcgagatca cacggctgat ggcggcgcgt tccgcaccga tcgagctgcc gaccgaaccg
     6601 gcgacgcccg acgccaacgc gctgcccggt cagccggcgc cgcgtccgct ggcgggaccg
     6661 atcgtgccgc tggtggcatc ctctgtcggc accgatcaac tgctcggcgg cccaggctcc
     6721 cgtccggccg cggttgacgc gctcgcggcg cgaacgctgg tcaagggcga accactggcg
     6781 ccacccgccg gccgcgccga cgatttcgtc tggccgcgcc gcgaggtcgg acgcgaacag
     6841 gccaagggcg aaacgccggt ggcatccgct tcgccggatg gaacgattgc ggcacctggc
     6901 gcggcgccga agccgcagca gagaaggcta cgcccggccc agtcgggccc gccggcgatg
     6961 cgggaattct tcggctttgg aaacgcgcca cgccaaccgc cgccgccgcg tgccgctcca
     7021 ggcgttctgc gccagccggg cacgatcggg cggtcggcgt gggtgccaga cttctttacg
     7081 cggtgatctc tcgcctccct tgccgggatg ccgagcttca ggtctcagct gtaataccgc
     7141 cagcgctgcg gcgggcggcg cggcggacgc gtcatcagca tcgccagcgc aaagccgatg
     7201 ccgccggcga ccaacagcgc gccgagcgga ttgtcctgca cctttttcgc caccacttcc
     7261 gaaccctcgc ggaaggtatc gccgctgttc tcataggcat ccttggcg
//
LOCUS       DATNOI010000316        12638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1624571,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000316 DATNOI010000000
VERSION     DATNOI010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12638
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624571"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="VN968_12375"
     CDS             complement(<1..1195)
                     /locus_tag="VN968_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL30070.1"
                     /translation="MRSMSLKSLSFSSGATSRPDATRSNLSTSLTLAAMSLGYGVVQL
                     DVTIVNTALNSIGTSLGGGVSELQWVVSSYTIAFAAFILTAGALGDRLGAKRIFMAGF
                     AVFTAASLACALAPNAAILIAARAVQGLGAAILVPNSLALLSHAYPDEKQRGRAVGIW
                     AAGASLALISGPLIGGGLIALIGWRSIFLVNLPIGLAGLWLSWRYATETPRHQEREVD
                     LPGQGAAIAALGCLAGAIIEGGTHGWRHPFVIAGLAASAVLAILFVVQERRAPQPMLP
                     LSLFEHRLFALTALVGLLVNVAFYGLIFVFSLYFQRVNGLSPFATGLAFLPMLGAVLP
                     VNLVAARVAERIGAPATIAAGAALSAIGCLAMLGIEPGTGYWAICAQLIAISTGLGLL
                     VPPLTS"
     gene            complement(1392..1898)
                     /locus_tag="VN968_12380"
     CDS             complement(1392..1898)
                     /locus_tag="VN968_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HXL30071.1"
                     /translation="MGRFTKDIRTMNDLFVHQLQDIYYAEKQLVKALPKMAEKATDKQ
                     LRQGFLTHLDETKIHVQRLERVFQMHGAEVKAVDCPAIDGIIEEADEVIGEVEDKSML
                     DAALINAAQAAEHYEITRYGSLIAWAKRLGRNDCASVLRKTLDEERATDKKLTTLAES
                     KINFRAAR"
     gene            2314..3387
                     /locus_tag="VN968_12385"
     CDS             2314..3387
                     /locus_tag="VN968_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HXL30072.1"
                     /translation="MHRLLTTLGRGFKKRIGWKRLGIVASLVIIAFAITTLVRTLKGV
                     DAGVILTALTEIPPAHIALAALCVLGAFCTLTFYDFFALRTIGKKNVPYRIAALSSFT
                     SYSIGHNIGATVFTGGAIRFRIYSDYGLSAIDVAKICFLSGLTFWLGNTFVLGIGMAL
                     HPWAASAMDQLPPAINRLIALGGIAAIVSYVVWLALGDDRRELGQNGWKVILPSAPLT
                     LLQILIGVVDLGFCALAMYLLMPAQPGIDFVSLAVVFILATLLGFASHAPGSIGVFDA
                     AMLVALPEFGREQLVATLVVFRILYFLIPFGIAISVMGTRELWLNVVLPWQERRKLHE
                     ALDEACPPSSAVPQPVKCRPRKG"
     gene            3475..5481
                     /locus_tag="VN968_12390"
     CDS             3475..5481
                     /locus_tag="VN968_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30073.1"
                     /translation="MIRIHIRSMILGALLVGALGALPTPWAAAQTAAAAAQGAPDAPM
                     QISWEVRNRFRLFREERDFLLHTDSARGRSILGSEQALELQSDGRGWARNTVNRLCID
                     LTGRVSEPCTRDNVKESYLTPSDHPVTLRLTGAIPVGATCAWSIDDGEGPPRQSTLDC
                     AEPLSFRARYGRPTMASVDVSSGPDAPQRVATEIMVRDIFIAGLGDSIASGEGNPDRP
                     IALSDEGFCFRSYLGTAGAQYYRPSRAGYKGGRACEAPDSLQVWQRQSALWFNSACHR
                     SLYSYQTRTALQLAVQYPHIAVTYLPLACTGATIPDGLFGGQRARECPPTKSTGCQAT
                     VNAQLAELRDAVAAAKRRQPDRNLDLVLLSIGANDIGFSGLVADVIVDTPTERVLFRR
                     TGVMGSVEDSRAALAQDLPQSFGKLREALKSLVGDMSHVVYVSYANPTLADGAPCPGG
                     RAGFDIHPSFNAQPPRLANVSGYVQNEFLPQLKALALCQSGILCRDPAGDRMTFVDGH
                     QAAFADHGFCARGPNDPEFDRECFSAKGDSFDPDIVTSASQPMVCGRSAGEYRAYLPR
                     ARWIRDANDSYFAAMTYPQGLPSSSQPADIHDATWGILSAVYGGAVHPSAEGHAAMAD
                     AAFPAAAAVLQLDAAVPEVTREPAAPIVTAPGARRPAGQPSGLY"
     gene            5575..6222
                     /locus_tag="VN968_12395"
     CDS             5575..6222
                     /locus_tag="VN968_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30074.1"
                     /translation="MLSYLNAETGFIRAFGNRSLLLIFLCALSRSGDHPGKASALGVS
                     LDFNPGLIVVFGPVLALLLLISLKIESDTLLVAREFVVGKMSHLDPGATKPSRWVFGL
                     FAVPCATATFMIVQFILNVVPAKPGCAGYVWTRKFTDFSVQGGTPSTYCIHNLTDGMP
                     WIYPPIQTYGYLACLAACFYLTYLIARNWVRVRPHEVEVSKPEVSRREASEPAVA"
     gene            complement(6294..8225)
                     /locus_tag="VN968_12400"
     CDS             complement(6294..8225)
                     /locus_tag="VN968_12400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXL30075.1"
                     /translation="MSARPLIPRRKLFDNPTFFGAKLSPDGRLISWLAPVDGVLNVWV
                     SPVDSIAAGLPVTRTRGRPINWQDWSPDGRYIMFLNDENGDENLHLFVVDPRNCELRD
                     LTPFANIRALPAYWSHIAPGKIAINLNDRDRRWHDVFLLDLATGQRSLIWENRQGFEW
                     VGLDWQLQPRHGRSHGSDGGARLWHLDGEQVTHWRDVPFDASWTTRLWSFDAAGKHLH
                     MLSCIEQDKSALVQIDWSTGEQNVVFESRRADVSDAIFNARTFEPEAVCIDPARQEWS
                     ALTSAVAPELALIKARLPGHAFHVQSQADDDRRWIVMSYTAEQPATYHLLDRDEQTLT
                     ELFTARPELKPYRLAPMHAVEGRSRDGLTLVSYLTLPADIEGDRPAAPLPMVLIVHGG
                     PWARDIFGYRGDHQWLADRGYAVLSVNYRGSTGFGKAFVAASEKQHARKMHDDLIDMV
                     DWAVGEGIAQKDKIAIFGVSYGGLASFIAATFTPEVFCCAVPVVGISNLQTLLESMPP
                     YWAGFAEYMYRSYGDPRTEEGRKLLAERSPINRVGNIGKPMLIFHGVNDVRCKVAESD
                     TIVAAMRARNIPVTYVVYPDEGHGFQKPPNRLSYIVMAEAFFARHLGGAFEPVGSDLE
                     GSSHEIRAGADILTDLGVA"
     gene            complement(8718..10670)
                     /locus_tag="VN968_12405"
     CDS             complement(8718..10670)
                     /locus_tag="VN968_12405"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3471 domain-containing protein"
                     /protein_id="HXL30076.1"
                     /translation="MLEVVVRAAICTLLLAVAVQLCLRTLRVRHPKLLLSAWTAVLVA
                     SVAMPALQRAIPSAAPTLLSRSFSAAGIVNSNAPASLLPLSMISSSLTSLTISPSGAT
                     TARASEPQRTFAWRKWLTGTYVLVASLFLLRLLLGLILSWRMLRATHPVRENWAAGKR
                     LRTSTWICAPVTVGSQVLLPAECVNWDARRRRAVLAHEEAHIARGDFYVQLLSQVNRS
                     VFWFSPLAWWLHRRLTALAELASDDAAIEALGDRPGYAAILLDFARLPRNPSFGVAMA
                     RPATVCQRIERILAEETTPAPVTRLLQTVIAAAVAPLAMLTAVLFAEAAPLEASREQV
                     APHTRITIDPKLLDAYAGFYLNAATDSLMIVTREDDHLLTRRAGNQPVAEYPYTDHDF
                     FLTVAPQQNSFVTDASGAVLRVVHHQWGRDETLERIGAEAAQRHEEARLKRVAEERIP
                     RTAISIDPQLLDNYVGAYQLKPQLVFTVTHEGDKLLAKLTGQPTYEVHPYTERDFFYT
                     IVAAQLSFVPGSDGKASAVVLHQNGKDQTAPRVDLSVAQALDVKLAEQSAPRTAVKID
                     SSLLDGYVGRYSNAELEITATREGDQLFVQVTGYGRYAIFPYTEHDFFATIQPTQYTF
                     ATDNTGKATQMVRHRRGKDEVFTRED"
     gene            complement(10670..11047)
                     /locus_tag="VN968_12410"
     CDS             complement(10670..11047)
                     /locus_tag="VN968_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HXL30077.1"
                     /translation="MNDRAPDLGELEREVMQLVWSAGETTADAIRERLARKPKESTVR
                     TVLRRLEEKGYVTHTVDGRTYVFRAAAARPQVAARAVKAIVDWLCNGSVEEVLVGMAD
                     ARMLDSRTLQRLAKRIEQAKGGK"
     gene            complement(11154..11711)
                     /locus_tag="VN968_12415"
     CDS             complement(11154..11711)
                     /locus_tag="VN968_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HXL30078.1"
                     /translation="MLLAFMVPGQAAEPSAAGLWQKIEDGKPVVWVLVVDHDGVFEGV
                     IAKTFPKPGEEIHETCAKCTDDRKNAPLLGISFIRNMKRDGLKYEDGNVLDPRDGKIY
                     KAKMSVSPDGQSLTMRGYWGISLLGKDETWFRLPDTEMASLDPAIVAKYLPAQAAAQA
                     AAKPPVAMVKPTAAAKKSASPQLLK"
     gene            complement(11987..>12638)
                     /locus_tag="VN968_12420"
     CDS             complement(11987..>12638)
                     /locus_tag="VN968_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL30079.1"
                     /translation="KRPPVPGIEAYEGTSVFYSVRKMEQFRDKDLLIVGGGDSALDWT
                     LNLHPVAKRVTLLHRRDDFRAAPHSVEQMRALVAAGKLDLKIGQVTGLEGEGGMLTGA
                     TVKGSDNAISRVGCDTMLPFFGLTMKLGPVANWGIALENNLVPVETAAFETSVPGIFA
                     IGDINTYPGKLKLILCGFHEGALMAQKAHRYVYPDKRLVFQYTTSSSSLQKKLGVN"
BASE COUNT         2330 a         4015 c         3907 g         2386 t
ORIGIN      
        1 tcgaggtgag cggcggcacc agcaaaccaa gaccggtgct gattgcgatc aattgcgcac
       61 agatcgccca gtagccggtg ccgggctcta ttccgagcat cgccagacat ccgatcgccg
      121 acagtgcggc gccggccgca atggtcgcgg gcgcgccgat gcgctcggcc acgcgcgcgg
      181 cgacgaggtt gaccggcaat accgcgccca acatcggcag gaacgcaagg ccggtcgcaa
      241 agggcgacaa tccgttgacg cgctggaagt acaggctgaa gacgaagatc aatccataga
      301 atgcgacgtt gacgaggagg ccgaccagcg ccgtcagcgc gaacagccga tgctcaaaca
      361 gcgacagcgg cagcatcggt tgcggcgcgc gccgttcctg cacaacgaac aggatcgcca
      421 gcaccgccga ggccgccagg cccgcgatca cgaacggatg gcgccagcca tgcgtgccgc
      481 cttcgatgat cgcgccggca agacaaccaa gggctgcgat cgccgcgccc tggcccggca
      541 aatcgacctc gcgctcctga tgtcgtgggg tctcggtggc atagcgccag ctcaaccaca
      601 ggccggcgag accgatcggc agattgacca ggaagatcga ccgccagcca atcaacgcaa
      661 tcaggccgcc gccaatcaac ggcccggaga tcagtgcgag gctggcaccg gcggcccaaa
      721 ttcccaccgc gcgaccgcgc tgtttttcgt ccgggtaggc gtggctgagc agcgcaaggg
      781 agttgggaac gagaatggcg gcaccgagtc cctggaccgc tcttgccgcg atcaggatcg
      841 cggcattggg cgcgagtgca caggcgagcg acgctgccgt gaaaaccgca aaacccgcca
      901 tgaagattcg cttggcgccg agccgatcgc cgagcgcgcc ggcagtcaga atgaaggcgg
      961 cgaaggcgat ggtgtaggag ctgacaaccc actgcagctc ggaaacgccg ccgcccagcg
     1021 aggtgccgat gctgttgagc gcggtgttga cgatggtgac atcgagctga acgacgccat
     1081 agccgaggct catggccgcc agcgtcagcg acgtcgaaag atttgaacgc gtcgcgtccg
     1141 gccgggacgt tgcaccagaa gaaaatgata gcgacttcaa ggacatactc cgcatagatc
     1201 acctcgattt cgaggaacca acgcggcaag aacgtatccg tttcggctga agccttcgac
     1261 cacgatcgaa gtgtcgttgc aaagcgcggg ttggtcgcga ttgcgcgtcg cgtccgccga
     1321 tcgcctcagc caacgcaaaa cgccgcgcgg aagattcccg gcgcggcgtt ttgtctcagt
     1381 tctgcctcag atcagcgcgc ggcgcgaaag ttgatcttgc tctcggccag cgtggtcaac
     1441 tttttgtcag tggcccgttc ctcatcgagg gtcttccgca ggacactggc gcaatcgttg
     1501 cggccaagcc gctttgccca cgcgatcagg ctgccgtagc gcgtgatctc ataatgctcg
     1561 gcggcctggg cggcgttgat caatgcagca tccagcatcg atttgtcctc gacctcaccg
     1621 atgacctcat cggcttcctc gatgatgccg tcgattgccg gacagtcgac tgccttcacc
     1681 tcggcgccat gcatctggaa cacccgttcg aggcgctgca catggatttt ggtttcatcg
     1741 agatgggtca gaaaaccctg cctgagctgc ttgtccgttg ccttttccgc cattttcgga
     1801 agcgccttca cgagctgctt ctcggcataa tagatgtcct gcagttggtg cacgaacagg
     1861 tcgttcatgg ttctgatgtc tttggtaaag cgtcccattg cgcggagcct ttttgtttgg
     1921 ggaacacacg gcttgtttgg ggaacacagc gacgcctttg tcgtggccgc cgcgccgata
     1981 tggccgatat gttcgattgc tgatgtgacg ctaaccggcg gatccccggg gttgtacctg
     2041 ccgcttgcga aaacgtcgcg cgatggaaca ggaacatcgt gaaaaatgga acggccggac
     2101 cgccgctgca cgagactcga attttggccg ttgcggcata tgtacggtgt gtgacagcca
     2161 gcgtcgatcc aaggccgaag ctggcgttgt caagggctgc acaacgacac gtttttgcct
     2221 tatgggtctt tgcaagtgga gcgattagcc gccgtttgcc gagggatcgc tttgatgggc
     2281 taccaacttg ttgccgccct gccgggagga cgaatgcacc gactgctgac cacgcttggg
     2341 cgtggtttca agaaacggat cggctggaaa cggctcggga tcgtcgcaag cctcgttatc
     2401 atcgccttcg cgatcacgac actggtccgt accctcaagg gcgtcgatgc cggcgtgatc
     2461 ctgactgcgc tgacggaaat accgcccgcc cacatcgcgc tcgccgcgct gtgcgtgctc
     2521 ggcgcgttct gtacgctgac gttctatgat ttcttcgcgc tgcggaccat cggcaagaaa
     2581 aacgtgccct accgcatcgc ggcgctgtcg agcttcacca gctattcgat cggccacaac
     2641 atcggcgcca ccgtattcac cggcggcgcg atccgcttcc ggatctattc ggactacggc
     2701 ctgagcgcca tcgacgtcgc caagatctgc tttctgtcgg gcctgacgtt ctggctcggc
     2761 aataccttcg tgctcggcat cggcatggcg ctgcacccct gggcagcctc cgcgatggat
     2821 cagctgccac ccgccatcaa ccggctgatc gcactgggcg gcatcgccgc gatcgtctcg
     2881 tacgtggtct ggctggcctt gggcgacgat cggcgcgaac tcggacagaa cggctggaaa
     2941 gtgatattgc cctcggcgcc gttgacgctg ctgcaaatcc tgatcggcgt ggtcgatctc
     3001 gggttctgcg cgctggcgat gtacctcctg atgccggcac agccggggat cgactttgtc
     3061 tcactggcgg tggtgttcat tctggcgacc ctgctcggct tcgccagtca tgcgccgggg
     3121 tcgatcggcg tgttcgacgc ggcaatgctg gtggcgctgc ctgaattcgg cagggaacaa
     3181 ttggtggcca cgctggtggt gttccggatc ctgtatttcc tgatcccctt cggcatcgcc
     3241 atttcggtca tgggcacacg cgaattgtgg ctgaatgtcg tgctgccgtg gcaggagcgc
     3301 cgcaagcttc acgaggcgct tgacgaggcc tgcccaccct cgtccgccgt gccgcagccg
     3361 gtgaagtgcc ggccgaggaa gggctaatgc cgcgccatct ggactccctt gtaaccccct
     3421 tggcttttgg gtgatctctc ttatttccgc cctatcggta acgtcaaacg cgtcatgatc
     3481 cgaattcaca tccgttcgat gatcctcgga gcgctgctcg tgggggcgct cggcgcgctg
     3541 ccgacaccct gggcggcggc gcagaccgct gccgcggcgg cgcaaggcgc gccagatgcc
     3601 ccgatgcaga tatcgtggga ggtccgcaac cgctttcggc tgttccgcga agagcgcgat
     3661 ttcctgctcc ataccgatag cgcgcgaggc cgcagcattc tcggttcgga acaggcgctg
     3721 gagctgcaga gcgatggccg cggctgggcg cgcaacacgg tgaaccgcct ctgcatcgat
     3781 ctgacgggcc gcgtcagcga accctgcacc cgcgataacg tcaaggaaag ctacctgacg
     3841 ccatcagatc atcccgtcac gctcaggctg accggcgcta tcccggtggg cgccacctgc
     3901 gcctggtcga tcgacgatgg cgaggggccg ccgcggcaat cgacgctgga ttgcgccgaa
     3961 ccgctgagtt tccgggcgcg ctatggccgt cccacgatgg cttccgtcga cgtctcgagc
     4021 ggacccgacg cgccgcagcg cgtcgcgacc gagatcatgg tgcgcgatat tttcatcgcc
     4081 ggccttggcg acagcatcgc atcgggcgaa ggcaatccgg accggccgat cgcattgtcg
     4141 gacgagggct tctgcttccg ctcctatctc ggaaccgccg gcgcccaata ttatcggccg
     4201 agccgcgccg gctataaagg cggccgggcc tgcgaagctc cggactcgct gcaggtgtgg
     4261 cagcggcaga gcgcgctctg gttcaattcc gcctgccatc gctcgctgta cagctatcag
     4321 acccgcaccg cgctgcagct tgccgtgcaa tatccgcata tcgcggtgac ctacctgccg
     4381 ctggcatgca cgggcgccac catccccgac ggcttgttcg gcggacaacg cgcgcgggaa
     4441 tgcccgccga ccaagtccac cggctgccag gccacggtca acgcgcaatt ggccgaattg
     4501 cgcgatgccg tcgccgccgc gaagcgccgg caacccgatc gcaacctcga cctggtcctg
     4561 ctgtcgatcg gcgccaatga catcggcttt tccggactgg tcgcggacgt gatcgtcgac
     4621 accccgaccg aacgcgtgct gtttcggcgc accggcgtca tgggatcggt agaggattcg
     4681 cgcgcggcgc tggcgcagga cctgccgcaa agtttcggca aattgagaga ggcgctaaaa
     4741 tccctggtcg gcgacatgtc gcacgtcgtc tatgtatcct atgccaaccc gaccctggcc
     4801 gacggcgcgc cctgccccgg cggacgcgcc ggcttcgata ttcatccctc gttcaacgca
     4861 cagccgccac ggctggccaa cgtgtccggc tatgtgcaga atgaattcct gccgcagttg
     4921 aaagcgctcg cgttgtgcca atcggggatc ctgtgccgcg atcccgccgg cgatcgcatg
     4981 accttcgtcg atggccatca ggctgccttc gcagatcacg gtttttgcgc gcgcggacca
     5041 aacgatccgg aattcgatcg ggagtgtttt tcggccaagg gcgacagttt cgatccggat
     5101 atcgtcacct ctgcctcgca gccgatggtt tgcggccgca gcgccgggga atatcgcgcc
     5161 tacctgccgc gcgcacgctg gatccgcgat gccaatgaca gctactttgc ggcgatgact
     5221 tatccgcaag ggctcccctc ctccagtcag cccgccgaca tccacgacgc cacctggggt
     5281 attctttccg cggtgtatgg cggcgcggtg catcccagcg ccgaaggcca cgccgcgatg
     5341 gcggacgcgg cgtttcccgc cgcggcggcg gtgctgcagc tcgatgccgc cgtgcccgag
     5401 gtgaccaggg agccggccgc gcccatcgtg actgcgcccg gcgcgcgccg tccagccggc
     5461 caacccagcg gattgtacta ggatctggtg gagcttcgcc aaatcagact gtcgtaatct
     5521 ccgggcagac gctgccagac tgacgttacc ttgtgtccga cgaatgaggc tgccatgcta
     5581 tcgtatctga acgctgaaac cggtttcatc agggccttcg gcaaccgcag cctgctgctg
     5641 atcttcctct gcgcgctttc gcgcagcgga gatcaccccg gcaaggcgtc ggcgctcggg
     5701 gtgtcgctcg atttcaatcc cggtcttatc gtggtcttcg gtcccgtcct tgcgctgctt
     5761 ttgctgatct cgctgaaaat cgagtccgac acgctgctgg tcgcgcgcga gttcgtcgtg
     5821 ggaaagatga gccatctgga tccgggcgcc acgaagccga gccggtgggt gttcgggctg
     5881 ttcgccgttc cctgtgcaac cgccacgttc atgatcgtcc aattcatcct caacgtcgtg
     5941 cccgcgaaac cgggatgcgc tggatacgtc tggacgcgaa agttcacgga tttttcggtc
     6001 cagggcggta ccccatcgac ctactgcatc cataacctga ccgatgggat gccgtggatt
     6061 tatccgccga ttcaaaccta cggatatctc gcctgcctcg ccgcgtgctt ttatctgacc
     6121 tatctgattg ccaggaattg ggtcagggtt cgtccacacg aagtcgaagt cagcaaaccc
     6181 gaagtcagca gacgcgaagc cagcgagccc gctgtcgctt agcctgcagc atcgcaccaa
     6241 cctcaccgcg gccacgcggc cggtcccgtg cgggcaaagc agggctgata tggtcaggcc
     6301 acgcccaaat ccgtcaggat atcggcgcct gcgcgaatct cgtggcttga gccttcaaga
     6361 tcggagccga cgggttcgaa ggcgccgcca agatgcctgg caaaaaacgc ctctgccatg
     6421 acgatgtagg aaagccggtt cggtggtttc tggaagccgt gcccttcatc cgggtaaacc
     6481 acgtaggtga cagggatgtt cctggcccgc atcgcggcga cgatggtatc gctttcggca
     6541 actttgcagc gcacgtcatt gacgccgtga aagatcagca tgggcttgcc gatattcccg
     6601 acgcggttga tgggagagcg ttcggcaagc agcttgcggc cttcctcggt gcgcggatcg
     6661 ccgtagctgc gatacatgta ttcagcaaag ccggcccaat agggcggcat cgactccagc
     6721 agggtttgca gattggagat accgaccacc gggaccgcgc agcagaaaac ctccggcgtg
     6781 aatgtagcgg ctatgaacga agcgagcccg ccgtaagaca cgccgaagat cgcgatcttg
     6841 tccttttgcg cgatcccctc accgacggcc caatcgacca tgtcgatcag gtcgtcatgc
     6901 atcttgcggg cgtgttgttt ttcgctcgcc gcgacgaagg ctttgccgaa cccggtcgag
     6961 ccacggtaat tgaccgagag cacggcatag ccccggtcgg ccagccattg atgatcgccg
     7021 cgataaccga agatatcgcg cgcccacgga ccaccgtgca cgatcagtac catcggcagc
     7081 ggcgcggccg ggcgatcacc ttcgatgtcg gccggcaacg tcaaatatga aacgagggtg
     7141 agcccgtcgc gcgacctgcc ctcgaccgcg tgcatcggcg caagacgata tggcttcaat
     7201 tccggtcgag ccgtgaacag ctcggtcaag gtctgctcat cccggtcgag cagatgatag
     7261 gtggcgggct gctcggccgt ataactcatg acgatccagc gacggtcgtc atcggcctgg
     7321 ctctgcacgt ggaaggcatg gccgggcagc cgcgccttga tcaaggcgag ttccggcgcg
     7381 accgcggacg tcaacgcact ccattcctgt cgcgcgggat cgatgcagac ggcctcgggc
     7441 tcgaacgtcc gggcgttgaa tatcgcatcg gaaacatcgg cacgccggct ttcgaacacg
     7501 acgttctgtt cgccggtcga ccagtcgatc tgcaccaggg ctgatttatc ctgctcgatg
     7561 cacgagagca tatggaggtg cttgccagcg gcgtcaaaac tccagagcct cgtggtccaa
     7621 ctggcatcga agggaacgtc acgccaatgc gtgacctgct cgccatcgag gtgccaaagc
     7681 cgggcgccgc cgtccgagcc atggctgcgg ccatgacgcg gctgcaactg ccaatcgagc
     7741 ccgacccact caaacccctg ccgattttcc cagatcagcg agcgttggcc ggtcgccaga
     7801 tcaagcagaa aaacatcatg ccagcgccgg tcacggtcgt tgaggttgat cgcgatcttg
     7861 ccaggggcaa tgtgcgacca ataggccggt agcgcgcgga tgttggcgaa gggcgtgaga
     7921 tcgcggagtt cgcaattgcg gggatcaacc acgaacaggt gcagattttc atccccgttc
     7981 tcatcgttga gaaacatgat gtagcgaccg tccgggctcc aatcctgcca gttgatcggc
     8041 cggcccctgg tgcgcgtgac cggcaagccg gcggcaatgc tgtcgaccgg cgagacccag
     8101 acgttcagca cgccgtcgac cggggcaagc cacgagatca aacgaccgtc tggcgacaat
     8161 ttcgcgccga agaatgtcgg gttatcgaac agtttccggc gcggaattaa gggacgagcc
     8221 gacattttcg gatctctgtc gtacttgggc atggccgaac gggggtcgtc tgacgccgag
     8281 ggctcgcttg ggtcagcttg aatggaagat cactacatct ttcgcatccc ggcgtattgc
     8341 ctgccgctgt aagggccgac atgaacgccg gtctgcatac attgcgcttt cgccgccggg
     8401 cgcatcggga accggtccgt catcgcgctg gaatcgcgaa tacacgaggc tgcatacgcg
     8461 ctgcatgtgt gagggattgc agccctcatc attgccatcc tgtatctata ggagcgccct
     8521 ccatcggagc tgccatagtg gccgtccatc ttgtaggcgg acgccggtgt gaaatcggac
     8581 gacagggcga tgccggatat tgcgagcgac aggataacag ctattggctt catcgtagtc
     8641 tccccatttt gtaccatcat gcaacaaccc aaagcctggg tgctcgaatg cccggttgac
     8701 gacgggcgcg ttcgggatca gtcctcccga gtgaacacct cgtccttgcc acgccgatgc
     8761 cggaccatct gggtcgcctt gccggtgtta tccgtcgcaa aggtgtactg ggtcggctgg
     8821 atggtcgcga agaagtcgtg ctcggtgtaa gggaaaatgg cgtatcggcc gtaaccggtg
     8881 acctggacga acagctggtc gccttcgcgg gtcgcggtga tttccagctc cgcattgctg
     8941 tagcggccga catagccatc gagcagactt gagtcgatct tgacggccgt gcgcggcgcc
     9001 gactgctcgg ccagcttgac gtccagggcc tgcgccacgc tcaaatcgac acgcggcgcg
     9061 gtctggtcct tgccattctg gtgcagcacg acggcgctgg ccttgccgtc ggaaccgggc
     9121 acgaacgaca gctgcgcggc aacgatcgta tagaagaaat cccgctcggt ataaggatgg
     9181 acttcgtagg ttggctgacc cgtgagcttc gccaatagct tgtctccttc atgcgtgacc
     9241 gtgaagacca gctggggctt gagctgatag gcgcccacat aattatccag cagctgcgga
     9301 tcgatgctga tcgcggtacg cggaatccgc tcttccgcca cgcgcttcag ccgcgcctcc
     9361 tcatggcgct gcgcggcctc cgcgccgatg cgctccagcg tttcgtcgcg gccccattga
     9421 tgatgcacca cgcgcagcac cgcgcccgag gcgtcggtga cgaagctgtt ctgctgcggg
     9481 gcgaccgtca gaaaaaaatc gtgatcggta tatggatatt cggccaccgg ctggttgccg
     9541 gcccgacggg tgagcagatg atcatcctcg cgggtcacga tcatcagcga gtcggtggcg
     9601 gcgttcaggt agaagccggc ataggcgtcc agcaatttgg gatcgatggt gatccgcgtg
     9661 tgcggcgcca cttgttccct ggatgcctcc agcggcgccg cttcggcgaa caacaccgcc
     9721 gtcagcatcg caagcggcgc aactgcggcc gcaatcacgg tctgcagcag cctggtgacc
     9781 ggagcgggcg tggtttcttc agccaggatg cgctcgatgc gctgacagac cgtagccggg
     9841 cgcgccatcg ccacaccgaa ggatggattg cgcggcaggc gcgcgaagtc gagcaggatc
     9901 gccgcatagc ccggacgatc gcccagcgct tcgatggcgg catcgtcgct cgccagttcc
     9961 gccagcgccg tcaagcgccg gtgcaaccac caggccagag gactgaacca gaacactgac
    10021 cggttcacct gcgacagaag ctggacatag aagtcgccac gcgcgatatg cgcctcttca
    10081 tgcgcgagga cggcccggcg cctgcgtgca tcccagttga cacattccgc cggcagcagc
    10141 acctggctgc cgacggtgac gggcgcgcag atccaggtgc ttgtccgcag ccttttgcca
    10201 gcggcccagt tttcccgcac cgggtgcgtg gcacgcagca ttctccagga caagatgagg
    10261 cccagcaaca gccgaagcag aaagagagag gccaccagga cataggttcc ggtcagccac
    10321 tttcgccagg cgaatgtccg ttgcggttct gatgcgcgcg ccgtggtcgc gccggagggg
    10381 gaaatggtca acgaagttaa cgacgacgag atcatcgaca aaggcaacag cgatgccggt
    10441 gcattggagt ttacgatgcc ggcagcagaa aatgaccggg agagcaatgt gggagcggcg
    10501 gatgggatcg cccgctgcag agccggcatc gccacggacg cgaccaatac cgcggtccag
    10561 gcggagagca gcaatttcgg gtggcgcacg cgaagcgtcc gcaggcacaa ttgaaccgcg
    10621 acggccaaca gcagcgtgca tatcgccgcg cgaacgacga cctccaacat cactttcctc
    10681 cttttgcctg ctcgatgcgc tttgcaagtc gttgcaacgt ccggctgtcg agcatgcgcg
    10741 cgtccgccat acccaccagc acctcctcga ccgatccgtt gcagagccag tcgacgattg
    10801 ccttcaccgc gcgggccgcg acctgcggcc gcgccgccgc agccctgaaa acgtaggtgc
    10861 ggccgtcgac ggtgtgggtg acgtatccct tttcctccag tcgccgcagc acggtgcgta
    10921 cggtcgattc cttcggcttg cgggccaagc gctcgcggat tgcatcggcg gtggtttctc
    10981 ccgccgacca caccaactgc atgacctcgc gttcgagttc accaagatcg ggagccctgt
    11041 cattcatcga tgcgccacct cgtgtaatgc tacgaacggt agcatatgcg cgacggcgac
    11101 atcacgtcaa gcccgggggc gctgaccggg cggctccgcc ttcgggcggc cgattacttc
    11161 agcagttgtg gcgacgcgct cttcttcgcg gcagcggttg gcttgaccat cgcgaccggg
    11221 ggcttggctg cggcctgcgc ggcggcctgc gccggcaaat atttcgcgac gatggcggga
    11281 tcgagcgaag ccatctccgt gtccggcaac cggaaccagg tttcatcctt gccgagcagc
    11341 gaaattcccc aatagccgcg catcgtgagc gactgaccgt ccgggctgac gctcatcttg
    11401 gctttataga ttttgccgtc gcgaggatcg agaacgttgc cgtcctcata tttcaggccg
    11461 tcgcgcttca tgttccggat aaaggaaatt ccgagcagcg gcgcgttctt gcggtcgtcg
    11521 gtgcatttcg cgcaggtctc gtggatctct tcgccgggtt tcgggaacgt cttggcgatc
    11581 acgccttcga agacgccgtc gtggtcgacc acgagaaccc acaccacggg cttgccgtcc
    11641 tcgatcttct gccatagccc ggcagccgac ggctccgccg cctgccccgg aaccatgaag
    11701 gccagcagca tcaccagaac agtcgtgacc tgtggcacca gcccaaatct ggaattggca
    11761 cgtcttgaat tggcaagtct ggaattggca agtctggaat taacagtcca catcatcatc
    11821 acctgaccaa cccctcgagc gatggcgtga gaataccgct attcggcgga tcgttccagt
    11881 gcaccgttcg cgtttcaagc ggtcttccgg agcgatcgga cagtgtcaca atcggacagt
    11941 gtcacaaatg tgccgtaaca gcttttttga atagcgcccg accaggtcaa ttgaccccga
    12001 gctttttctg caggctggag gacgacgtcg tgtactggaa caccagccgc ttgtccggat
    12061 agacataacg gtgcgccttt tgtgccatca gcgcgccttc gtggaacccg cacaggatca
    12121 gcttcagctt gccgggatag gtgttgatat cgccgatcgc aaaaattccg ggcacgctgg
    12181 tctcgaaggc cgccgtctcg accggcacca gattgttctc cagcgcaatg ccccagttcg
    12241 cgaccggccc caacttcatg gtcaggccga agaacggcag catcgtatcg cagccaactc
    12301 ttgagatggc gttgtcgctg cctttgacgg tggcgcctgt gagcatgccg ccctcgcctt
    12361 caagtcccgt gacctggccg attttcaaat ccagttttcc ggcagccacc agcgcgcgca
    12421 tctgttcgac gctgtgagga gccgcgcgaa aatcgtcgcg ccggtgcagc agggtgacgc
    12481 gtttggcgac cgggtgcaga ttgagcgtcc agtccagcgc ggagtcgcca ccgccgacga
    12541 tcagcagatc cttgtcgcgg aactgctcca tcttgcgcac cgagtagaac accgaggtgc
    12601 cctcataggc ctcgataccc ggcaccggcg gccgcttc
//
LOCUS       DATNOI010000317         3505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_22209,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000317 DATNOI010000000
VERSION     DATNOI010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22209"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..837)
                     /locus_tag="VN968_12425"
     CDS             complement(40..837)
                     /locus_tag="VN968_12425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL30080.1"
                     /translation="MNLDLTGKIALVTGSTRGIGLASAAGLAQMGAEVIVNGREAAAV
                     ADAIARIKQAAPGATAHAAVFDLGGAKGCAGLVAQFPEVDILVNNLGIYEPKAFFDIE
                     DDDWTKMFEVNVMSGVRLTRHYLKRMLDHKEWGRVVFVSSESAIFVPKEMVHYGFSKA
                     AQLYVARGAAEQTKATNITVNAVLPGPTWVEMAPVRLAARAKGLGTTADELAARIFTE
                     RRPASLLQRYATPEEVANLICYVCSKAASATNGAALRVDGGIVTNPF"
     gene            complement(884..2509)
                     /locus_tag="VN968_12430"
     CDS             complement(884..2509)
                     /locus_tag="VN968_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXL30081.1"
                     /translation="MSTASILPATSRMRRRPTIRRLAAWLAARASDPTASLWLVIGFA
                     AAHAVLWTLILINLKAAQDVHMDVAEAFAWGQKFQFGYGKHPPLAGWVAGVWFGIFPV
                     ANWATYALAMATLGCGLVVCWLISLRVVDRRRAFFVVVMLALYPVFNFKGFKYNPDLL
                     QLVTLPLLVLAYLHAFEKRSLRSGLWLGLAGALALMTKYWVLTMIGAIGIAALLHPDR
                     LRFLRSPAPWVAIVTLVVVMIPHLVWLKEVDFVPLTYAGDVYGLASRAQSIQLILGYI
                     SHNLALLAVPVALAALALAWTPRWWTILARQPLAPFTSAWSRGANASVNASQALNVWI
                     IQIVVAVGPPLGGLIFTVYMKTDWGISLFFLTPLALVAIPSLRLHKMALFYIAAIWLV
                     ITLAVLAAAPTIAARELAANPNGASGYGARSELARELTQAWQTRFHTRWAVVAGTTEI
                     GEPMTFYSPDHPAPFTPGEIWSSGLTSLEEAKRLGFIGVCDTSDNRLPVCEAWMAANA
                     DNAERLAITTQRFFHGHPGPAISWKVYIVPPSK"
     gene            2624..>3505
                     /locus_tag="VN968_12435"
     CDS             2624..>3505
                     /locus_tag="VN968_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXL30082.1"
                     /translation="MTSIPISNIRNFAIVAHIDHGKSTLADRLIQTTGGLQAREMKEQ
                     VLDSMDIERERGITIKAQTVRLSYPAKDGKTYILNLIDTPGHVDFAYEVSRSLAACEG
                     SLLVVDASQGVEAQTLANVYHALDAGHEIVPVLNKVDLPAAEPEKIRQQIEDVIGIDA
                     SDAVMISAKTGLGVPDVLEAIVTRLPPPKGDREATLKALLVDSWYDVYLGVVVLVRVV
                     DGTMKKGSRIRMMGTGAAYDVERVGFFTPKMAQVDELGPGEIGFITAAIKEVADTRVG
                     DTITDERNPVDEPLPGFK"
BASE COUNT          706 a         1123 c         1071 g          605 t
ORIGIN      
        1 tcgcttccgc ttgcctatgt gccggccatg acgggtaatt cagaacggat tggtgacgat
       61 gccgccatcg acgcgcaagg ccgcgccgtt ggtcgcgctc gcagccttcg aacagacgta
      121 gcagatcagg tttgccacct cttccggggt cgcgtagcgc tgcagcagcg acgccggccg
      181 gcgttcggtg aatatccgcg ccgcaagttc atcagccgtc gttcccagcc ccttggcgcg
      241 agccgcaagc cggaccggcg ccatctcgac ccaggtcggg cccggcaaaa ccgcgtttac
      301 cgttatattg gttgccttgg tttgttcggc tgcgccgcgc gcgacataaa gctgcgcggc
      361 tttcgagaag ccgtaatgga ccatctcttt cggtacaaag atcgcggatt cgctggatac
      421 gaacacgaca cggccccact ccttatgatc cagcattcgc ttcagataat gccgggtaag
      481 gcgcacaccg ctcatgacgt tgacctcgaa catcttggtc cagtcatcat cctcgatatc
      541 gaaaaaggcc tttggctcat agattccaag attgttgacg agaatgtcga cctcggggaa
      601 ttgcgcgacg agtccggcac agcctttggc gccgccaaga tcgaagacgg ccgcatgcgc
      661 agtggcgccc ggcgctgcct gcttgatcct ggcgatcgcg tcagcaacgg cagccgcctc
      721 ccgcccgttg acgattactt cagcgcccat ttgcgcgagc ccggcggcgg aggctaatcc
      781 aatgcctcgg gtcgagccgg tgacaagcgc aatctttcca gtcagatcga gattcatatg
      841 ttgatttcct gagattcccg ttcgccggtc cggttgtgaa agcttacttc gacggcggca
      901 cgatatagac tttccagctg atcgcgggac caggatgccc gtggaagaac cgctgcgtgg
      961 tgatggcgag ccgctcggcg ttatcggcat tcgctgccat ccaggcctcg cagaccggca
     1021 gccggttatc tgacgtatcg cagacgccga tgaacccgag ccgcttggcc tcctcgagcg
     1081 atgtcaaccc ggacgaccag atctcgcccg gcgtgaacgg cgccggatga tccgggctgt
     1141 agaacgtcat cggttcgccg atctcggtgg tgccggcgac cacggcccat cgggtgtgga
     1201 aacgtgtctg ccaggcctgg gtcagttcgc gggcaagctc agaacgcgcg ccatagcctg
     1261 aagctccatt gggattcgca gccagctcgc gggcggcgat ggtgggcgcc gcggcgagca
     1321 ccgcgagtgt gatcacgagc cagatcgcgg cgatgtaaaa cagtgccatc ttgtggaggc
     1381 gcagcgacgg aatcgcgacc agcgccaacg gcgtcaagaa gaacagcgag atgccccaat
     1441 cggttttcat gtagaccgtg aaaatcaagc cgccgagcgg cggtccgacc gccaccacga
     1501 tctggatgat ccagacattg agcgcctgcg atgcattcac gcttgcgttc gcaccgcgcg
     1561 accatgcgct tgtgaacggc gccaacggct gccgcgccag gatggtccac catcgcggcg
     1621 tccatgccag cgccaatgcc gccagcgcta ccggcaccgc aagcagcgcc agattgtggc
     1681 tgatataacc gaggatgagc tgaatgctct gcgcgcggct cgccagcccg tagacgtcac
     1741 cggcataagt cagcggcacg aagtccacct ctttcagcca gaccaggtgt ggaatcatga
     1801 cgacgacgag cgtgacaatc gcgacccatg gcgccggcga gcgcaggaac cgcagccgat
     1861 cgggatgaag gagcgcggct attccgatgg ctccgatcat cgtcagcacc cagtatttgg
     1921 tcatcagcgc cagcgcgccg gccaggccaa gccacaggcc tgatcgcaac gagcgcttct
     1981 cgaacgcatg cagataggcc agcaccagca gcggcagcgt caccagctgc agcagatccg
     2041 gattatattt gaagcccttg aagttgaaga ccggatagag cgcgagcatc accacaacga
     2101 agaaggcgcg gcggcgatcg accacgcgca agctgatcag ccagcagacc acgaggccac
     2161 agccgagcgt cgccatcgcc agcgcgtaag ttgcccagtt ggcgacgggg aaaatcccga
     2221 accagacacc cgcgacccat cccgccagcg gcggatgctt gccatagccg aactggaatt
     2281 tctgccccca ggcgaaggct tccgcgacgt ccatatgaac gtcctgcgcg gctttgagat
     2341 tgatcaggat cagcgtccac agcacggcat gcgcggcggc aaaaccgatc accagccaca
     2401 ggctggctgt tgggtcgctg gcccgcgccg caagccatgc cgcgagccgc cggatggttg
     2461 gccggcgccg catacgcgac gttgcgggca aaattgatgc agttgacatg accttgtgcg
     2521 tagcgcgttt ttgacgtcag tggaatcagc ttggcgtgaa gaagccccgt aaaaacggcg
     2581 cattatggtt gccgccccgg agcgcgaatg ctatcccgcg cccatgacat ccatccctat
     2641 ttccaacatc cgcaacttcg ccattgttgc gcatatcgac catggcaaat cgacgctggc
     2701 cgaccgcctg atccagacca ccggcggctt gcaggcgcgc gagatgaagg aacaggtgct
     2761 cgattcgatg gatatcgagc gcgagcgcgg catcaccatc aaggcgcaga ccgtgcggct
     2821 aagctatccg gccaaggacg gcaagaccta catcctcaac ctgatcgaca cgcccggcca
     2881 cgtcgacttc gcctacgagg tttcccgttc gctggcggcc tgcgagggct cgctgctggt
     2941 ggtcgatgcc agccagggcg tcgaggcgca gacgctcgcc aacgtctacc atgcgctcga
     3001 cgccggccac gagatcgtgc cggttctcaa caaggtcgac ctgcctgccg ctgaacccga
     3061 aaagatcagg cagcagatcg aggacgtgat cggcatcgac gcctcggatg ccgtgatgat
     3121 ctcggccaag acgggcctgg gcgttcccga cgtgctggaa gccatcgtca cccgcctgcc
     3181 gccgccgaaa ggcgatcgcg aggcgacgct gaaggcgctg ttggtcgata gctggtacga
     3241 cgtctatctc ggcgtcgtcg ttctcgttcg cgtcgttgac ggcacgatga agaagggcag
     3301 ccgtatccgc atgatgggta cgggcgcagc ctatgacgtc gagcgcgtcg gcttcttcac
     3361 gccgaagatg gctcaggtcg atgaactcgg cccaggcgag atcggcttca tcaccgccgc
     3421 gatcaaggaa gtcgccgaca cgcgcgtcgg cgacaccatc accgacgagc gcaatccggt
     3481 cgacgaacca ttgcccggct tcaag
//
LOCUS       DATNOI010000318         6011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1150365,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000318 DATNOI010000000
VERSION     DATNOI010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150365"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="VN968_12440"
     CDS             <1..790
                     /locus_tag="VN968_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30083.1"
                     /translation="ACKLTPEPIIVARGVTRRFGGLVALDGLDLDVARGIVQAIIGPN
                     GSGKTTLLNALSGASHADAGSIRIGGNEIFHLKPHRIARLGLARTFQTIRIFENLSVI
                     ENVKVAAACNTPTSLLNIIASNTRQKHSEAEIDRHSHEALEIVGLAGRANDHARQLPY
                     AQQRLLEIARALASKPNVLLLDEPAAGMNMTESMTLLDTIARLKGSGITILLVEHNVR
                     LVMGISDRVTVLDFGKKIAEGTPSEVQRNPLVIEAYLGRRHRHA"
     gene            783..1493
                     /locus_tag="VN968_12445"
     CDS             783..1493
                     /locus_tag="VN968_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30084.1"
                     /translation="MLKIENLRVSYGHIEVLKGISFSVEMGEIVALIGGNGAGKTTTL
                     STISGLVRPTSGTISWKGENIQTAAVEAIVGSGLAHCPEGRRIFPGLTVHENLITGTA
                     SRSYRKRDIDEDLALVFDLFPRLKERLKQGGWSLSGGEQQMLAIGRALMSRPSLLMLD
                     EPSLGLAPIVIEQVFDRIVELKKRTGLGVLLVEQNSAMALEIASRAFVLETGMITLSG
                     PSGTLADDPRIREAYLGG"
     gene            complement(1521..1871)
                     /locus_tag="VN968_12450"
     CDS             complement(1521..1871)
                     /locus_tag="VN968_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30085.1"
                     /translation="MPIENHPSNGDVSSPGLDSQHLDNACGASGAGHHRNAGLGLFGL
                     LFRIASWMASEVLAGCAAYAEAMYPMYTPQDEQPDSSDAAGSQQTAEVIQIKRVSESR
                     NPSDIQGRKGRLKS"
     gene            complement(2077..3051)
                     /locus_tag="VN968_12455"
     CDS             complement(2077..3051)
                     /locus_tag="VN968_12455"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HXL30086.1"
                     /translation="MLLSCGDALIDFLPVKSADGRDALVPVVGGSCLNIAVGMARLGA
                     PAGFVGGISTDLFGRMIADHAIASQVELRYATRSEHQTTLAFVRTVAGEPQYAFYDEG
                     TASRNWIYRRGSIPFDQIEAIHVGSTTLADDNGAAQVLAMLEDARGSVSISFDPNCRP
                     KLVRHKARYVERMNAFAAIADIVRMSDVDFEFLYGGSDYSGRAKSLMEAGASLVVVTR
                     GIRGAQAWHKEAGQVEVEAPTVDVMDTIGAGDSFQAALLFALRAIGRIAAGSLAQADS
                     GELRRALSFASTCAAFTCARAGADPPRETDVGAELSRLLDPIERTAPP"
     gene            complement(3056..4720)
                     /locus_tag="VN968_12460"
     CDS             complement(3056..4720)
                     /locus_tag="VN968_12460"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HXL30087.1"
                     /translation="MRQAFIGIDVGTSSARAGVFDETGKLLATARRPITVWHEAGSVV
                     EQSSSEIWAACAASVRAAMAEAVLPPSAVRGLGFDATCSLVVLDAGANPLTVSASGDD
                     RRNIIVWMDHRATAETRFVNDTHDDVLRYVGGSISPEMEIPKLLWLKRHLPSTYNKAG
                     HFFDLADYLSFRATGSMLRSICTLACKWNFLAHEQRWSGSYFERIGLGDIASDGYAKI
                     GKQTVAPGTPLGTGLTESAARDLGLLEGTPVGASLIDAHAGGVGTIGGRAGSGQPVDV
                     RRRLAYIMGTSACIMATTSEPCFVPGVWGPYFSGMVPGFWLNEGGQSAAGAAIDHLIR
                     SHPAYNEAAATARAGGMELLEFLERRIVARSQNPGEAAWFARDIHVLPEFLGNRSPFA
                     DPDSRAVVAGMDLDADIGSMERLFVAGLCGLAYGLADVVDTFRSHGVDSDLMVISGGA
                     GRSALVRQIMADTTGLTVAVPETQEPVLLGAAMLGAVAGRSYGSIGEAMASMSAIGCL
                     SEPTAPGMAEFHRTKRQVHGLMRKLDRESRDAMRGIESIAGVDTKN"
     gene            complement(4749..5522)
                     /locus_tag="VN968_12465"
     CDS             complement(4749..5522)
                     /locus_tag="VN968_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL30088.1"
                     /translation="MYLEKFRLDGKTAVVTGAGQGIGLACAEALAEAGAKVVIADRDP
                     IAAETGRASLQAKGLNAETVIMDVTDSARVSEVADQLASRHGKIDILVNNAGIARSET
                     PAEKVTDEHWLNVVDVNLNGTFWCCRAFGKHMLEAKSGSIVNIGSMSGFIVNKPQEQS
                     YYNASKAAVHHLTKSLAAEWGARGVRVNAVAPTYVATPLNAFVKNNPQMYDAWIGGTP
                     MARMGEVDEIASVVLFLASDAASLMTGSVVLVDGGYTCW"
     gene            complement(5580..>6011)
                     /locus_tag="VN968_12470"
     CDS             complement(5580..>6011)
                     /locus_tag="VN968_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HXL30089.1"
                     /translation="EAMTMADKIVVLNAGRIEQYGSPLELYERPANLFVAGFIGSPKM
                     NFVSGEAAGEPGVATLGIRPEHLKIGKEGDGWPGTVSVAEHLGSDTFLYVDAGKLGTL
                     TVRAIGEFGLKAGDRVWLSPDPARLHRFDKDGGVIQAVSEK"
BASE COUNT         1161 a         1929 c         1799 g         1122 t
ORIGIN      
        1 cgcgtgcaaa ttgaccccgg agccgatcat tgttgcgcga ggcgtgacgc ggcgatttgg
       61 cggcctcgtc gcgcttgatg gcctcgacct cgacgtggcc cgaggcattg tgcaggccat
      121 catcggtcca aatggctcag gcaagaccac gctgctcaac gcgctgagcg gcgcctcgca
      181 cgccgacgca ggatcaattc gcattggcgg caatgagatt tttcacctca agccacaccg
      241 catcgcgcgc ctcggtctgg cccgtacctt ccagaccatc cgaatttttg aaaacctgag
      301 cgtcatcgaa aatgtaaagg tggcggcggc ctgcaacacg ccgacgtcgc ttttaaacat
      361 tatcgcatcg aatacgcggc aaaagcacag cgaagccgag atcgatcgtc actcgcacga
      421 ggcgctggag attgtcggcc tcgccggccg ggcgaacgac cacgccaggc aattgcccta
      481 cgcgcagcaa cgcctgctgg agatcgcgcg agcgctggcg tcgaagccga atgttctgct
      541 gctcgatgag ccggccgcgg gcatgaacat gaccgaaagc atgacgctgc tggacaccat
      601 cgccaggctc aaaggcagcg gcatcacgat actgctggtc gagcataatg tccggctggt
      661 gatgggcatc agtgaccgcg tcaccgtgct cgacttcggc aagaagatcg cagaaggcac
      721 gccttccgag gttcagcgca atccgctggt gatcgaagcc tatctcggca ggcggcaccg
      781 gcatgcttaa gatcgaaaac ttgagagtat cctacggcca catcgaggtg ttgaagggca
      841 tttcgttcag cgtcgagatg ggcgagatcg tcgcgttgat cggcggcaat ggcgccggca
      901 agacgaccac gctgagcacg atctccgggc tggtgcgccc gacctccggc acgatctcct
      961 ggaagggcga aaacattcaa accgccgcgg tcgaggccat cgtcggttcg ggtttggcgc
     1021 attgccccga aggccggcgt atttttccgg gcctgaccgt gcacgaaaat ctcatcaccg
     1081 gcacggcgtc gcgttcttac aggaagcgcg acatcgatga agatcttgct ttggtgttcg
     1141 atctgttccc gcgcctcaag gagcggctca agcagggcgg ctggtcgcta tccggcggcg
     1201 agcagcagat gcttgccatc ggccgcgcct tgatgagccg tccgagcctg ttgatgctgg
     1261 atgaaccctc cctcggcctc gcgccgatcg tgatcgagca ggtgttcgat cgcatcgtcg
     1321 aactcaaaaa acgtaccggc ctcggcgtgc tgctggtcga gcaaaattcc gccatggcgc
     1381 tggaaatcgc cagccgcgct tttgtgctgg aaaccgggat gattaccctg tccggaccct
     1441 cggggaccct cgccgacgac ccgcgcatcc gcgaggccta tctcggcggc tgaacaagcg
     1501 gcggcgggac tccaagatag ctaggacttc aatcgcccct tccgaccttg aatatcagag
     1561 ggattacggc tttcggatac gcgtttgatt tgaatgacct cggccgtttg ttgagagcct
     1621 gcggcatcgc tggaatccgg ttgctcatct tgtggtgtgt acatcgggta catcgcctcc
     1681 gcgtaggcgg cgcagccggc gagaacttcg gaagccatcc atgacgcgat tctgaacaag
     1741 aggccgaata agccaagacc agcgtttcga tggtgacccg cgccggacgc accacaagcg
     1801 ttgtccagat gttgcgaatc caacccgggc gacgatacgt cgccgttcga cggatgattt
     1861 tcgatgggca ttgatctctc cagctctcaa actggagaat ttttccatgc caatgttgct
     1921 gcactgttcc ggcatcgcaa cgaaaaggct acggttgtaa ccaggtacgc ggccggttca
     1981 atttgacgat gcgaagcgtt cccatgcaga aatttcagtc gcgatccaat tgcccgcgct
     2041 gcgaaagatt tttcgcgggc gcttgcggcg ccatgctcat ggcggcgcgg ttcgttcgat
     2101 cggatccaga agccgggaca attccgcgcc gacgtcagtt tctcgtggcg gatcggcacc
     2161 ggcgcgtgca catgtgaaag cagcacaggt cgatgcgaac gaaagcgcgc ggcgaagttc
     2221 gccggaatcc gcctgcgcca gcgatccggc tgcgatccgt ccgatggctc gcaaggcaaa
     2281 cagcagggcc gcctggaaac tgtcgccggc cccgatggtg tccatcacgt ctaccgtggg
     2341 cgcctcgact tcaacctggc ccgcttcttt atgccatgcc tgcgcccctc gaattccgcg
     2401 cgtaacgacc accaggcttg cgcccgcctc catcagcgat ttggcccgtc ccgaataatc
     2461 gctaccacca tacagaaatt caaagtcgac atcggacatc cgcacgatgt ccgcgatggc
     2521 ggcaaatgca ttcatccgct cgacatagcg ggccttgtgc ctgaccagct tgggcctgca
     2581 attcggatcg aaggaaatgc tgaccgatcc gcgcgcatcc tcgagcatgg ccagcacctg
     2641 ggctgccccg ttgtcatcgg cgagcgtggt cgacccgaca tgaatggctt cgatctgatc
     2701 gaaggggatg gagccgcgcc gatagatcca gtttctggac gcggtcccct cgtcgtagaa
     2761 ggcatattgc ggctcgccgg cgacggtgcg cacgaacgca agcgtcgtct ggtgctcgct
     2821 ccgcgtcgca tatcgaagct cgacctggga ggcgatggca tggtcggcaa tcatgcggcc
     2881 gaacaggtcg gtcgaaatcc cgcccacaaa tcctgccggc gcgccgagac gcgccatgcc
     2941 gacggcgatg ttgaggcagg accccccaac cacaggcacg agcgcgtcgc gcccgtccgc
     3001 cgacttgacg ggcaggaaat caatcaaggc atctccgcaa ctcagcagca tcgttctagt
     3061 ttttcgtatc gacaccggcg atcgattcga ttccgcgcat cgcatcgcgg ctttcccgat
     3121 ccagttttcg catcagtcca tggacctgtc gcttggtgcg gtggaattcg gccattccgg
     3181 gcgccgtcgg ttcgctcaaa caaccgatcg ccgacatcga ggccatcgcc tccccgatcg
     3241 atccataaga gcggccggcc accgcaccca gcatggcagc tcccaggagg accggttcct
     3301 gggtttcggg aaccgcgacc gtcaggcccg tcgtgtccgc catgatctgg cggaccagcg
     3361 cactgcgtcc cgcgccgcca ctgatcacca tcaagtcgct atcgacaccg tgcgaccgga
     3421 acgtgtcgac gacatccgca aggccatagg cgagcccgca cagtcccgcc acgaatagcc
     3481 gctccatcga gccgatgtcg gcgtcgagat ccatcccagc gacgaccgcg cgcgaatcgg
     3541 gatcggcgaa cggcgagcga ttgccgagaa actccggcag aacgtgaatg tcgcgggcaa
     3601 accatgccgc ctcgcccggg ttttgcgaac gcgcgacaat gcgccgctcg agaaattcta
     3661 gcagttccat gccgccggcg cgcgcggtcg cggcggcctc gttataggcg gggtgggacc
     3721 tgatgagatg atcgatggcc gcgcccgccg cggattgacc gccctcgttg agccagaatc
     3781 ccggaaccat tccggaaaaa taggggcccc aaacgcccgg aacgaagcaa ggctcggacg
     3841 ttgtcgccat gatgcaggcc gaggttccca tgatgtaggc cagacggcgg cgtacatcga
     3901 ccggctgacc cgaccccgcg cgccctccga tcgtgcccac acctccggcg tgcgcgtcga
     3961 tcagcgacgc gccaacgggc gtcccctcca gcagaccgag gtcgcgcgcg gcagattcgg
     4021 tcaggccggt gcccagcggc gtgccgggtg cgacggtctg ctttccgatt tttgcgtagc
     4081 cgtccgacgc gatatctcca agaccgatgc gctcgaaata gctgccgctc cagcgctgtt
     4141 cgtgggcgag gaaattccat ttgcaggcga gcgtgcatat cgaacgtagc atcgatccgg
     4201 tggcgcgaaa cgaaagataa tcggcgagat cgaagaaatg accggctttg ttgtaggtcg
     4261 acggcagatg ccgcttcagc caaagaagtt ttggaatttc catctcgggc gatatcgagc
     4321 cgccgacata gcggagaacg tcgtcatgcg tgtcgttgac gaaacgggtt tccgcggtcg
     4381 cccgatgatc catccagacg atgatattcc gtcgatcgtc gcccgacgcg ctgaccgtca
     4441 gcggattggc gccggcgtcc agcaccacga gcgagcaagt cgcatcgaaa cccaggcccc
     4501 ggacggcgga aggcggaagg acggcttccg ccatggccgc ccgaacggaa gcggcacatg
     4561 ccgcccagat ctcggatgaa gactgttcga caacgctccc cgcctcgtgc cagaccgtaa
     4621 tcggacgccg ggccgtcgcc agcagctttc ccgtctcgtc gaaaactccg gcgcgcgcgc
     4681 tcgatgttcc gacgtcgatg ccgataaaag cctgcctcat cgcaaccccg ttcaagatca
     4741 gcgcaagctc accagcacgt atagcctccg tcgacgagaa cgacgctgcc ggtcatcagg
     4801 cttgcggcat cagacgccag aaacaggacg acggaggcga tttcgtcgac ctcccccatc
     4861 cgggccatcg gcgtgccgcc gatccacgca tcatacatct gcgggttgtt cttgacgaat
     4921 gcattaaggg gggtggcaac gtaggtcggc gccaccgcgt tgacgcgcac gccgcgggcc
     4981 ccccactcgg ctgcgagcga cttggtgaga tggtgcactg cggccttcga ggcgttgtag
     5041 taggactgct cctgtggctt gttgacgata aagccggaca tcgaaccgat gttcacgatg
     5101 gaacctgact ttgcctccag catatgcttg ccaaaggcgc ggcaacacca gaacgtgccg
     5161 ttgagattga cgtcgacgac gttcagccaa tgctcatcgg ttaccttttc agcgggggtc
     5221 tcgctccgcg cgatgcccgc attgttgaca agaatgtcga tctttccgtg ccttgacgcc
     5281 aattgatccg ctacctcgga gacgcgcgcg gaatccgtta catccataat gacggtctcg
     5341 gcattcaagc ctttcgcctg caggctcgcg cggccggtct cggcggcgat ggggtcgcga
     5401 tccgcgatca cgaccttcgc tcccgcctct gccagcgctt ccgcgcacgc aagtccgatt
     5461 ccctgaccgg cgccggtcac cacggccgtt tttccgtcca gcctgaattt ttccagatac
     5521 atcttctttg ttcctactcg caggtcggcc atcggcgaaa ttcacttcgc cggtatcgat
     5581 tacttttcgc tcaccgcctg aatcacccct ccgtccttgt cgaagcggtg caggcgggcc
     5641 ggatcgggcg agagccagac gcggtcgccg gctttcaggc cgaactctcc gatggcgcgc
     5701 acggtgagcg ttccgagttt gccggcgtcg acatagagga aggtgtcgct gcccagatgc
     5761 tccgcgaccg acacggtccc gggccagccg tctccttctt tgccgatttt cagatgttcg
     5821 ggcctaatgc ccagggtggc aacgcccggc tcgccagccg cctctccgga aacgaagttc
     5881 atcttgggcg atccgatgaa gccggcaacg aacaggtttg ccggccgctc gtataattcg
     5941 agcggcgagc cgtactgttc gatgcggccc gcattgagca cgacgatctt gtcagccatc
     6001 gtcatggcct c
//
LOCUS       DATNOI010000319         2071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2501998,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000319 DATNOI010000000
VERSION     DATNOI010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2501998"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="VN968_12475"
     CDS             complement(<1..1105)
                     /locus_tag="VN968_12475"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HXL30090.1"
                     /translation="MRLRDLFSDDATIDRQAEAAVVTGLAVDSRAVKPGDLFFALAGS
                     RTDGARFIDSAVSSGAVAVAADHPPHGGSRVPFVVTPNPRRALALAAAKFYPRQPETI
                     AAVTGTSGKTSVAAFTRQIWQRLGHASASIGTIGLVSPKRTLYGSLTTPDPIALHRQL
                     DEIARDGVTHLAFEASSHGLDQFRLDGVRIRAAGFTNLSRDHLDYHPDILHYLAAKLR
                     LFRDLLAADGAAVISVDHDCSQAVMDAARAKNLRIVAVGRKGDGAGEGIRLVEAAIDG
                     FAQKLVLEHRGRRHAVRLPLVGEFQIENALVSAGLAIGTGSNPDQVFESLEHLEGAKG
                     RLERVGERNGAPIFVDYAHKPDALAKALQALRPY"
     gene            complement(1147..>2071)
                     /locus_tag="VN968_12480"
     CDS             complement(1147..>2071)
                     /locus_tag="VN968_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXL30091.1"
                     /translation="NPREAHDPERINRLTTGVYEMGSTFKALTLAMALDSGKAGLGTM
                     FDVRGALHYGKFAIHDTHPLGRSISLSEVFTFSSNVGAARIALAQGVEAHKAFLRKMG
                     QLDRLRTELPESASPIVPKRWSELNTITIAFGHGLSVAPLQAVMGINAVMNGGYLIPP
                     TFLKRSEAEARALAKKVIKSETSEKMRYLMRLNAEIGTAKQADVKGYYIGGKTGTSEK
                     VVNGRYSKKQVLNSFTAIVPADNPQFQVLVMLDEPKALPETHGFITSGWNAVPTGGKV
                     IARIAPLLGIEPRFDLPPSDRLILAASKTTH"
BASE COUNT          355 a          665 c          670 g          381 t
ORIGIN      
        1 cgtagggccg caatgcctgc agtgccttgg ccagcgcgtc gggcttgtgg gcgtaatcga
       61 cgaagatcgg cgcgccgttg cgctcgccga cccgctccag ccggcctttt gcgccctcga
      121 gatgttcgag ggattcgaac acttgatccg ggttgctgcc ggtgccgatc gcgaggcctg
      181 cggacaccag cgcattctcg atctggaatt cgccgaccag cggcagccgg acggcatgtc
      241 tgcgcccgcg atgttcgagc acaagcttct gcgcaaagcc gtcgatagca gcctcaacca
      301 gtcgaatgcc ctcgcctgcg ccgtcgccct tgcggccgac cgcgacaatc cgcaagtttt
      361 tcgcacgcgc cgcatccatg accgcctgcg aacaatcatg gtcgacggat atcaccgccg
      421 cgccatccgc cgccaacaga tcgcggaaca gccgcagctt ggcggcaaga taatgcagaa
      481 tgtccggatg gtaatccagg tggtcacgtg acaggttggt gaagccggcg gcacggatgc
      541 gcacgccatc cagccgaaac tgatcgagcc cgtgcgatga cgcctcgaag gccagatgcg
      601 tgacgccgtc gcgcgcgatt tcgtcgagct gccgatgcag cgcaatcgga tccggcgtgg
      661 tcagcgaccc gtagagcgtg cgcttcggcg ataccagccc gatggtgccg atgctggccg
      721 aggcgtgacc gagccgctgc cagatctgcc gcgtaaaggc cgcgaccgag gtcttgccgc
      781 tggttccggt tacagccgcg atggtttcgg gctgtcgcgg atagaatttc gcggcagcga
      841 gcgccagcgc gcggcgcgga ttgggcgtga cgacgaacgg cacccggctc ccgccgtgcg
      901 gcggatgatc ggccgccacc gcgactgcgc cggaggaaac cgcagaatcg atgaagcgcg
      961 cgccatcggt cctgctgccg gcgagcgcga agaacaggtc gcccggcttt accgcgcggc
     1021 tgtccacggc gaggccggtg accacggccg cttccgcctg gcggtcgatt gtggcgtcat
     1081 cgctgaacag gtcgcgaagt ctcattatct tcatcttcca gtccggccgg cgcgtgatgt
     1141 tacgccttaa tgggttgtct tcgatgccgc aagaataagg cggtcggacg gcggcagatc
     1201 gaagcgcggc tcgatcccca gaagcggtgc gatgcgagcg atcaccttgc cgccggtcgg
     1261 caccgcgttc catcccgagg taataaagcc gtgggtttcg ggcagcgctt tcggctcgtc
     1321 cagcatcacc aggacctgga actgcggatt gtcagccgga actatcgcgg taaacgagtt
     1381 cagcacctgc ttcttggaat accgcccatt gacgaccttt tccgaggtgc cggtcttgcc
     1441 gccgatatag tagcccttga cgtcggcctg cttggcggtt ccgatctcgg cgttgagccg
     1501 catcagatag cgcatctttt cgctggtctc cgacttgatc actttcttcg ccagcgccct
     1561 ggcttccgct tccgatcgct tgaggaatgt cggcggaatc agatagccgc cgttcatcac
     1621 ggcattgatt cccatcaccg cctgcagcgg cgccaccgac aggccgtgac cgaaagcaat
     1681 ggtgatggtg ttcagctcgc tccagcgctt cggcacgatc ggcgaggcgc tttcgggcag
     1741 ttcggtgcgc agccggtcga gctggcccat cttgcgcaag aatgccttgt gggcctcgac
     1801 gccctgcgcc agcgcgatac gcgcggcgcc gacattcgac gaaaatgtga atacttccga
     1861 cagcgagatc gagcggccca gcggatgggt gtcgtgaatg gcgaatttgc cgtagtgcag
     1921 cgcgccgcgg acatcgaaca tggtgccgag accggccttg ccggaatcca gcgccatcgc
     1981 cagcgtcagc gccttgaagg tcgagcccat ctcgtagacg ccggtggtca gccggttgat
     2041 gcgctcggga tcgtgggctt cgcgcgggtt a
//
LOCUS       DATNOI010000320        27783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1620247,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000320 DATNOI010000000
VERSION     DATNOI010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 27783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27783
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620247"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VN968_12485"
     CDS             complement(<1..752)
                     /locus_tag="VN968_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HXL30092.1"
                     /translation="MNANSRRSKPVSHQPIAAASLHAPLARLLRPLVRLCIHGGMTFP
                     ALAQLLRELFVNVAEHDFALEGKEQTDSRVSLLTGIHRKEVARLRGAGTPVNETPATL
                     SLTSAIIARWLAAPEFTDAKGDPLLLPRTASGDAPSFEQLVSSVTKDVRPRAVLDEWL
                     DRKLVTINDDEEIVLVDTAFVPRGDDERKWHYLGRNLHDHIAAAAENVSGPAPRFLER
                     AVHYDGLSAKLAKRLEGRSRELAVDALKIANR"
     gene            868..2076
                     /locus_tag="VN968_12490"
     CDS             868..2076
                     /locus_tag="VN968_12490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXL30093.1"
                     /translation="MLARSRRLSVLHIFYDCYPDLFGGALSVIRDICAGLKDSFTACV
                     LVCSRSSGQRRIVVDGLTVERVRSFGDVCSLPAAPTYPLRLWRRVHDHDLLALHAPFP
                     LADLMFALGLGRDRPLVVHYHADIVSHAGLRRLVEPLVRRTLRRAEAIVVSNAILLRS
                     SPLLREFAEKCHVVPFGVDVSKYDWPVQRPDDDDAERGRGRLVLACGQLVPYKGFDVL
                     VRAAVGQRFEVWIIGEGPERARLERMIRSLGVADRVRLLGSAPHAKLAKFMCTADVFV
                     LPSVTNAETFGIAQLEAMAAGRPIINTSLDTAVPRVARHGIEAITVPPGNHAQLAEAI
                     ETLLRDPERRRRMGHAARLRATTQYSIDAFRKGIETVYRHAVTTKASSRSALASRPGT
                     IGTGPENRHC"
     gene            2212..2865
                     /locus_tag="VN968_12495"
     CDS             2212..2865
                     /locus_tag="VN968_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30094.1"
                     /translation="MYSESMPLVEKEWGGRPLDLDARSDQRIGKRPMKPSIRSPLPSG
                     LKDDHVASDRPSIGRRIFRTLTRFSIAVLIGIGATLAWQSHGDAARDMVVARAPSLAW
                     LLSVSTTKSPAMAATSPDPAQQLEPLASNLDVVRRSVEQLAAKQEQMAQNMATLQAVA
                     DDIRQRMSITPPSSPSQQQAAPIPQHKPPQPRAQSSAVQSSSVPRPLPPAGAPLLSR"
     gene            complement(3109..4320)
                     /locus_tag="VN968_12500"
     CDS             complement(3109..4320)
                     /locus_tag="VN968_12500"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL30095.1"
                     /translation="MSVNSNPSSPPRPDVPRLLSRARIIAEIARARARETEANRRISD
                     DLIESMHDAELFRILQPEAYGGFEYGFDVFADLVATIGRGCGSSAWVYGLGAVHQWFA
                     ACLPKEAQDEFWAEPGAIAAGSYAPVGKTVPVDGGYRTSGVWSFASGCDNARWYFLAT
                     MVAPFDGSEATRPSFLLVPSADVRIDDNRHTMGLAGTGSKNIVADDLFVPAHRALAIA
                     DLAAGTSPGTLVNTNPLYRQSFLSVVPVTLVAPVLGMAEGALADFVDMVTGRTTRGAV
                     GGGNRRMAEFATIQARLAEATGCVDAARLMMFHNLAETYAVAACGEPTPLDMRLRNRL
                     GHTFCTRLLVQAVDALFLAAGGQGIFLEKPLQRAWRDAHAAAAHISLNWDAVATMYGQ
                     HALGLEPKGQY"
     gene            complement(4356..5540)
                     /locus_tag="VN968_12505"
     CDS             complement(4356..5540)
                     /locus_tag="VN968_12505"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30096.1"
                     /translation="MPAVAGGGAMLRAVICASALAMTLVDGASAQEPLKIGLIQSLTG
                     PFSTVGKAAVNGAHLYVQRHGDVVAGRRVELVVSDDASAPDRAKRTAQEMIVNDRVAI
                     VCAGITPSALAIAPLVTEAKIPTVVMVSGASITVERSPYMVRTSFTLGQSSTVMADWV
                     AQNGARKIVTIVNEWAPGLESETEFKNRVATSNARIVDSLRMPLANPDFAPFLQRARD
                     LGPDTLFVFPANQAAIFAKQFLERGMDKSGIRLVGTGDLTDDDELPGMTDAALGIITA
                     HHYSALHDSQLNRTFVEAFQAAYGRRPNYHSVAGYDGMHLIYEALKKTSGNVDGNALV
                     AAMKGMSWESPRGPISIDPETRDIVQNEYIRRVERVNGQLYNVEFATIEAVKDPMKAA
                     KK"
     gene            5860..6798
                     /locus_tag="VN968_12510"
     CDS             5860..6798
                     /locus_tag="VN968_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXL30097.1"
                     /translation="MDSDILHKLEQRLGPIHARQRLGIETDHEAQVFGQGRTFFHLEN
                     LPSAPAIIRNALKLTGLYWRACRNAERVIVKRNELKFAALPSLFDGYTILHISDLHVD
                     MSEAAMQRLIELVDGLPYDLCVLTGDYRGKTFGPFEAALAGVASVRAHLRLPIYGVLG
                     NHDTIQMVPAMEAMGIPMLLNECEAIARGDQRIYLAGIDDAHFFRVDNIEKAASQIPK
                     EGFSILLSHTPEVYRQAAHANFNLMLSGHTHGGQLCLPGSIPIKLEAVLPRRMGAGAW
                     QYHNMSGYTSVGAGSSVVPVRLNCPPEITLHCLRRG"
     gene            complement(6920..7324)
                     /locus_tag="VN968_12515"
     CDS             complement(6920..7324)
                     /locus_tag="VN968_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HXL30098.1"
                     /translation="MPPVSILLNVFWIVFGGLWMAAAWVIAAFIMAITIIGLPWARAA
                     FNIAVYTLLPFGQRAVRRDSLTGRPDIGTGPLGLIGNVIWLVLAGWWLALAHLVTAIA
                     LAVTIIGIPFAWAHLKLAGIALWPIGKVIVPA"
     gene            complement(7398..8000)
                     /locus_tag="VN968_12520"
     CDS             complement(7398..8000)
                     /locus_tag="VN968_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HXL30099.1"
                     /translation="MTSLLYHQGNRAFQDQFDSRRLADRLEEKLARTVFSDDDKIFIE
                     SCIYFFVATADASGHPDCSFKGGAPGFVRITGPSELAFPDYDGNGMFKSVGNLAVNPH
                     VGLLFIAMHGNPKRLRINGEARIERDDPLLADTIGAQLIIRIKVNVIFPNCPRYIPSM
                     TLTEPSVYVPHAGVAPVEPAWKGFDAFRDVVPPRAGLQSG"
     gene            complement(8670..9155)
                     /locus_tag="VN968_12525"
     CDS             complement(8670..9155)
                     /locus_tag="VN968_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016556604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXL30100.1"
                     /translation="MAKIEARLTALGLVLPPAFSPPPGVVLPFVNVRLIGNRAVIAGH
                     GPQSPEGPLAQPLGKVGRELTLEQGYMAAKLTALSMLGYLKRTLGDLDRISHWVRILG
                     MVNSAPGFVQQPAVINGFSDLILELFGPEAGAHARSAVGMAELPFGMPVEIEGEVQIS
                     A"
     gene            complement(9166..10236)
                     /locus_tag="VN968_12530"
     CDS             complement(9166..10236)
                     /locus_tag="VN968_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL30101.1"
                     /translation="MMILSGDPPRSRVLSIVLVVIIAALAVTPLVFPGAKAMNVATKI
                     CIFAALVASYDLLLGYTGSVSFAHTMFYGIGSYSIAIALYSMGPSWAAVATGVVVGLP
                     LAMLLALAIGLFSLRVEAIFFAMITLAVASAFLVLASQLSWLTGGEDGRSFQLPELLR
                     PGTVLISKDRFGFELNGRVLTYYLIFVAAAAMILALLRVVNSPFGRVLQAIRENRFRA
                     EALGFRTVFHLTYANCIAALVAASAGMLNALWLRYAGPDTSLSFSIMLDILLMVVIGG
                     MGTIYGAIVGATIFILAQNYLQALMGAASNAAADAGLPLLPGLLHPDRWLLWLGLLFI
                     ASVYFFPTGVVGRLRSAARKSS"
     gene            complement(10233..11232)
                     /locus_tag="VN968_12535"
                     /pseudo
     CDS             complement(10233..11232)
                     /locus_tag="VN968_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616038.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
     gene            complement(11229..11951)
                     /locus_tag="VN968_12540"
     CDS             complement(11229..11951)
                     /locus_tag="VN968_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30102.1"
                     /translation="MTDLLTLAGVHTHIGRYHILHGIDFAVPEGQTTMLLGRNGAGKT
                     TTLRTIMGLWQASSGRISLDGQAIEHSATPDIARLGVGYVPESMAVFSDLTVKENLVL
                     AARDGPLDDSRLEWIFGFFPALRRFWLSRAGSLSGGQKQMLSIARAIVEPRKLLLIDE
                     PTKGLAPAIVVALIECFAEVKRHGATILLVEQNFHAARELGDRVLVMDNGLIVHRGEM
                     AALATDVTLQERLLGLSLETHQ"
     gene            complement(11948..12715)
                     /locus_tag="VN968_12545"
     CDS             complement(11948..12715)
                     /locus_tag="VN968_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30103.1"
                     /translation="MTFTLETRDLTIRFGGHVAVNRVSCTFRPGELTAIVGPNGAGKT
                     TYFNLISGQLRPSDGRILLDGADITQLSAPLRTRAGLGRAFQLTNLFPNLSVEENVRL
                     AVQSESGVHYEMLRPWTKRRDLIARADAILDKVALGGRRRVPATALSHGDQRKLEVAL
                     MMALEPKVFMFDEPTAGMSIDEVPVVLNLIAQLKQDASKIILLVEHKMDVVRSLADRI
                     IVLHNGQLVADGKPADVIASPIVQEAYLGIAPGKSAA"
     gene            complement(12900..14081)
                     /locus_tag="VN968_12550"
     CDS             complement(12900..14081)
                     /locus_tag="VN968_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXL30104.1"
                     /translation="MRTTLIVQAIAVALLGTAAQADDLKIALIYGKTGPLEAYAKQTE
                     TGLRMGLEYATKGTMTLDGRKIVVVTKDDQSKPDLSKAALAEAYQDDKADIAIGTTSS
                     AAALADLPVAEENEKILIVEPAVADQITGEKWNRYIFRTGRNSSQDAISNAVAIGKAG
                     VTIATLGQDYAFGRDGVAAFKQALAKTGATLAAEEYVPTTTTDFTAAGQRLFDALKDK
                     PGHKIIWVIWAGGGDPLTKLQDMDPKRYGIELSTGGNILPALAAYKRLPGMEGATYYY
                     YDIPKNPVNDWLVAEHQKRFNAPPDFFTAGGFSAAMAAVAAITKAKSTDTEKLISAME
                     GLEFDTPKGKMIFRKEDHQALQSMYHFKVKIDPNLAWAVLEPVRELKIEDMDIPIRNK
                     R"
     gene            14276..14914
                     /locus_tag="VN968_12555"
     CDS             14276..14914
                     /locus_tag="VN968_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase"
                     /protein_id="HXL30105.1"
                     /translation="MKYLSAAIGLAILIATSHVGRAEDITRFAPLKAEELTPAQKAWA
                     DMIAAPPRNAKFVNPPYRAYIRNPDLAPRLSALSDYLRWNTSLPPRLSELAILITARQ
                     WTAQYEWFAHYPLALKGGLDPKVAADIAAGKRPDNMRDDEAALYDLATALYRDRKVSD
                     PIYKAALEKFGERGIMDIIGIIGYYDLVSMTLITMQAEAPNDSVPPLQALSK"
     gene            complement(14936..16720)
                     /locus_tag="VN968_12560"
     CDS             complement(14936..16720)
                     /locus_tag="VN968_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXL30106.1"
                     /translation="MHSRAKHDPRTAATEAGGGSAVATELSVLRDVFRLLPGGVTVQD
                     GQGRFLLVNDAAAAQLGIAVGGQAAPDSQELTHRRETGLELLRAGRAAVIEESVTGSE
                     GKQTLLTAHRPVRIADRDLLLSSSADISEQKAIEAQLFRSAYYDELTGLPARRVIEHR
                     ANGLLQRDETPARFALAFLDIDNFKQINDYYGHAIGDALLVEVAKRIGLNLRESDMLS
                     RISGDEFLLLLDPIRGEHEMTEYIDFVLQRLRAPFFIDGSEIFASASIGVGLCPEHGR
                     SFDQLCQNADIAMYSVKNDGKGAAAFFDAGMEREALARMKVEQSLRLAILEKRFCCAF
                     QAKVDIRTQEIKGIEALVRLRDDDGVIQAPGTFVNLAVELGLIDELTHLVLAEIVKSI
                     DLINDTFGPDTSISINVAAKQAGNPQFMRPFALALEATGYPKRFMIEVTEDAFVAKTH
                     FQDEILPMFRKLGVGVSIDDFGIGYSSLSALADITADEIKIDRSFITDIHKRPRSQGI
                     LRAIESLSEALGMTVVAEGIETFEELAYLQAATKIRYAQGYYFSRPIFLEELRPPVRI
                     LGHARASAASRPHQESRSVYARNERYRG"
     gene            complement(16872..17834)
                     /locus_tag="VN968_12565"
     CDS             complement(16872..17834)
                     /locus_tag="VN968_12565"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXL30107.1"
                     /translation="MVKLIEEFRRGWHGISQPTPIFNVGFAAACLALSTAARWGFALI
                     RPDIFFTPYIPAVFFATALGGLRIGIATAIVGGTLGATVSFSNAPADFTRMVLLATYL
                     IVCGLTIWGIEHYRSIVSQQREISGRLIREEEYRKLIVDEFQHRLKNKLSTIHAVLHL
                     VLQDQPQIWAGIDQRLRAMSATDDLIAKVDGSGCDIRDLLLSELGPYGHVRFTLNGNP
                     LFLPAKLAVSLALIFHELATNAGKYGAFSSARGLLQVSWSVSGDRLNITWDETEGPPV
                     ASVGEAGFGTKLLKGALTPFDGKSEISFLKTGVHCTMQCRIPLA"
     gene            18023..18865
                     /locus_tag="VN968_12570"
     CDS             18023..18865
                     /locus_tag="VN968_12570"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXL30108.1"
                     /translation="MGRLDGKVAVITGATSGIGLRTAEIFVAEGAKIVIAGRRAPEGQ
                     ALAGKLGVNCVFRQTDVTVEDEMRALIGQAVEKFGRLDCLFNNAGGPAQTGGIEGLEV
                     ARFDAAMATLVRSVMLGMKHAAPHMRRQGSGSIINNGSIAGRLAGFSSSMVYGAAKAA
                     VIHLTKCVAMELGESGIRVNSISPGAIATGIFGKALGLSVEAAEKTSDVMREVYKTAQ
                     PIPRAGLPEDIAHAAVFLASDESSFINGHDLVVDGAITGGRNWTQQQQGYVALRKAFD
                     QGAG"
     gene            18957..19460
                     /locus_tag="VN968_12575"
     CDS             18957..19460
                     /locus_tag="VN968_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL30109.1"
                     /translation="MSTTAAMMQTRPEARPLYAAMIAGLACAFVIGKTLPIMMDTLSM
                     VLAPLCAVGAPSGSMPDAIEPISSHALPNVPGKRVTIVRVFYGPGGFTPAHRHAGSVT
                     AYITKGEIRSQLAGGPVETFKVGQSFFEPPGATHLVSANASATETAELIAVFVADEGA
                     QLTTFVE"
     gene            19545..20585
                     /locus_tag="VN968_12580"
     CDS             19545..20585
                     /locus_tag="VN968_12580"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL30110.1"
                     /translation="MNARLERKLRSLAALVVAGLIAGIVFNLTQGRTSPTYLAVGASY
                     GLLMSVTLGSTELFVLDGPMREWLGGLSFATSLMVRSTVYVAIITVIQWFQLGEVISG
                     VSPDTSSRAFWFSFIYSVVLSVVMNLALGVINIIGPRAFLNFFIGRYHTPVEEKRFVL
                     FVDIAGSTGLAERLGGIAIHRLLDRTFRLLTVAVVDYRGEVLDYVGDEVIVTWPERGG
                     AIDCRPLRCFVAMRDELSRASGQLEREFGAAPRIRGSLHFGPVIVGEIGDVKRAIVFN
                     GDVMNTAARLEELSRNVEGGFLASRAAMERFSSAPPFAVRDLGRLPIRGRADGIDVVG
                     IGAQPLPDLASI"
     gene            complement(20591..21970)
                     /locus_tag="VN968_12585"
     CDS             complement(20591..21970)
                     /locus_tag="VN968_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXL30111.1"
                     /translation="MSMQSAASPAITFTPPKRNSLTHIPGDEGWPLIGKTLEVLADPK
                     GQVERQAAKYGLVYRSHIFGETSLVLLGPEANELVLFDQAKQFSSTHGWGRILGRLFP
                     RGLMLLDFEEHRLHRRALSVAFKSGPMKSYLAALDTGIAARVAQWKAQPGPMLFYPAM
                     KQLTLDLAATSFLGAEIGPEVDEITRAFVDMVAASVAVIRKPLPGTAMARGVRGRKRI
                     VAYFSEQIPIRRAKGGGEDLFSQLCHATHEDGALLSTQDIVDHMSFLMMAAHDTLTSS
                     LSSFVGALAAHPQWQTRLRDEVMALGVAAGEATTFDNLEAMPLSEMAFKEAMRLKPPV
                     PSIPRRTIRDLTFKGYAIPAGTMVGVNPLFSHHMPEIWPDPERFDPMRFTDEAQRGRH
                     RFAWVPFGGGAHMCLGLHFANMQAKCFARHFLQNLSVSLEPGYTPNWQMWPIPKPRDG
                     LRVTLSPVA"
     gene            complement(22190..23431)
                     /locus_tag="VN968_12590"
     CDS             complement(22190..23431)
                     /locus_tag="VN968_12590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXL30112.1"
                     /translation="MARLKFGAFLAPHHPIGEHPMLQFRRDLDLVEQLDALGYDEFWC
                     GEHHSSGWETIASPEMFLAAAGERTKRIKLGTGVVSLPYHHPFNVAQRMVQLDHMTGG
                     RAIFGSGPGALASDAHTLGIDPMTQRDRQDEAIAIIRRLFNGERVTARSDWFTMNDAA
                     LQILPLQEEMPFVVASQISPSGMTLAGKYGIGIISLGSMSTQGLMALPTQWGFAEDAA
                     RKHGTTVSRSDWRVLLSWHIAETREQAQREAGAGLMRWHNEYNVGTLQRPGLQRFTSP
                     EDAVEKTAGGEAAASTIGTPDDLVKTIKSLMDVSGGVGTIVGFVHDWANPENTRRSWD
                     MVARYVVPEINGYLDGLRKSQKFVIENRAVFERAGQAVMAKIMENDKAAAALPLTGPG
                     RVAIPTINAPDLQKEAAKQKA"
     gene            23790..24038
                     /locus_tag="VN968_12595"
     CDS             23790..24038
                     /locus_tag="VN968_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30113.1"
                     /translation="MVHRTDIAGTVGCSRGRVDHGDDGSCRHAPSGALVMVLSSKEVI
                     YRIPATPYFDGEKCSRVFLTALPSVHAIYVETVGPMRR"
     gene            24106..25827
                     /locus_tag="VN968_12600"
     CDS             24106..25827
                     /locus_tag="VN968_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXL30114.1"
                     /translation="MTFHGRYLGGCAALAVAAILTASPTHAQDAAMLNAEAAQNDWST
                     HHGTYKSYHYSGLEQINADNVKNLEVAWTHYPERATRGLQSTPLAMDGVLYYSGSYSR
                     VYALDGATGKTIWTYAPELDEELVAKQTHSPYNRGIALGQGNLYVGTVDGRLIALNQK
                     TGKPVWDLKLLDSKKVTVGFTGAPLVVKDMVIIGAQGGEWPGRGPIFGVDAKTGQKKW
                     QFDTVAGTEEAMATWGNDSWRTGGGGGWMPGAYDPETNSVWWGTANPAPLYDWSGADW
                     KKSGPRPGDNLYTTSVIALDPDTGKLKFYHQELPHDAWDFDSAVGEFVMINRGDKKLV
                     VHANKSGFVFVYDRSNAKIENVWPLVKNINFVKSIDPKTGELIGRRDLVAGKAETPLC
                     PAIAGGISWNSGAYSPKTGLYYKIGQEWCMELTVLKTTPILEPMAQLNIGADFKLVAP
                     PDGAARGHLDARDPVTGVLKWEVNYKQPPLASVLATAGNLVFVPDAEGVVHAYSADSG
                     KELWSHNNGMGHNAGIISYMAGGKQYIAVPAGWGSLVADEFVALYGEPFKSMAKNTGA
                     LVVFALK"
     gene            25895..26254
                     /locus_tag="VN968_12605"
     CDS             25895..26254
                     /locus_tag="VN968_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXL30115.1"
                     /translation="MRILFVIVASLAIIWPWLPTTAAAEQSTSAGSASPPAQPEASSS
                     DIDGEQMFATTCGFCHQDGGRAAGRGPKLSKTERSDEFIIERIKNGKMGAMPAFGSVF
                     SDGQIIAILAYIRGLDD"
     gene            26247..27125
                     /locus_tag="VN968_12610"
     CDS             26247..27125
                     /locus_tag="VN968_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXL30116.1"
                     /translation="MISAGAKRHRERLGERLGERLGERLGDVRTAAWKPRYNLVRYAA
                     SICLLALGSATADARSLEIITERGALTLCANPNALPFASKTGPLPGFQIELGEKIAQQ
                     LGVKLTREWVISAIQYRRADCDLVLDTIARKDTPPAGGVQVSRPYHRSGVVLAVRADS
                     QASSLASLGADQRVGVPVGSLVSMTLGKGGTVTSPFVFEDDIVAALANREIEAAAVTP
                     VTVGWFNLQHADKPLRLIPAFDNDQDLNWNIAAGLFRPDDKLRARVDAAIEALLADGT
                     IARIYGRYGIELRPPQ"
     gene            complement(27151..>27783)
                     /locus_tag="VN968_12615"
     CDS             complement(27151..>27783)
                     /locus_tag="VN968_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30117.1"
                     /translation="QGVFDRGRLVASHIYRQVVEGPGGGDVLKISVVSAGVRDTVERI
                     GQALRWHGALSFDYIQDDATRTPHFIDANPRLVEPMNAWLSGVDLPGALLRVSLGETP
                     PVQPDGREGVLTRLGLMGLLDAARRRNRRRDILREIGLLALGLGRYRGSREELVPLLT
                     DPWCAVPLGVVVARLLHAPETATRFSETTVAAYSLTAPAIRQLRAWHDAA"
BASE COUNT         5301 a         8667 c         8708 g         5107 t
ORIGIN      
        1 tcccggttgg cgatcttcag ggcgtccacg gccagctcgc gcgaacgccc ttcgagacgt
       61 tttgcgagct tggccgaaag accgtcataa tgcaccgcgc gttcgaggaa acgcggtgcc
      121 ggcccggata cgttttccgc cgccgccgca atatgatcat gcaggttgcg gccgagatag
      181 tgccatttgc gctcgtcgtc gccgcgcgga acgaaggcgg tgtccactaa cacgatctcc
      241 tcatcgtcgt tgatggtcac gagcttgcga tccagccatt catccagcac cgcgcgcggg
      301 cgcacatcct tcgtgaccga actcacgagc tgttcgaacg agggcgcatc accgctggcc
      361 gtgcgcggca acagtagcgg atcgcctttc gcgtccgtga actccggagc ggcgagccag
      421 cgcgcgatga tcgcgctggt caatgacaag gtggcgggcg tttcattgac cggcgtaccg
      481 gcgccgcgca gccgcgcgac ctccttgcgg tggattccag tgagcagact aacgcggctg
      541 tcggtctgct cctttccctc caaggcaaag tcgtgctcgg caacgttgac gaagagttca
      601 cgcaacagct gagccagcgc gggaaacgtc attccgccat ggatgcatag ccgcacgagc
      661 gggcgcagca ggcgcgccaa cggcgcgtgc aggctcgccg cggcaatcgg ctgatgcgaa
      721 accggcttcg acctccggga attggcgttc atggcgaaac tgtagcggcg ggttccgtgg
      781 gatacaatcc cacttttctg tcgggatcaa ttgacgtgtg aaagtttccc acatatacag
      841 ggccaatcag tcatggagag ttcgcccatg ctcgcccgct ctcggcgcct ctcggtgctt
      901 cacatattct atgactgtta tcccgacctg ttcggcgggg ccctgtcggt gatccgcgac
      961 atatgcgcag gactgaaaga ctcgttcacc gcttgcgtgc tggtctgctc caggtcaagc
     1021 gggcaacggc gcatcgtagt ggacggccta acggtcgagc gcgtgcgctc gttcggcgat
     1081 gtatgttcgc ttccggccgc gcccacgtat ccattgcggt tatggcgccg ggtccacgat
     1141 cacgatctgc tcgcgcttca tgcgccattt ccgctggccg atctcatgtt cgcccttggg
     1201 ttgggccgcg atcgacctct ggtcgtgcat taccatgcgg acattgtttc gcatgccgga
     1261 ttgaggcggc ttgtggaacc gctggtgcgc cggacgctgc gccgggcgga agccatcgtc
     1321 gtgtccaatg ccattctgct gcgcagcagc ccgctgctgc gagaattcgc tgagaaatgt
     1381 cacgtcgttc cgttcggcgt cgatgtctcg aagtatgatt ggccggtgca gcgccccgac
     1441 gatgacgacg ccgaacgcgg tcgcggacgg ctcgtgctcg cctgcggaca actcgtcccc
     1501 tacaagggct tcgatgtgct ggtgcgagcg gccgtcggcc agcgttttga ggtctggatt
     1561 atcggcgagg gtccggaacg ggcccggctt gaacgaatga tccgtagcct cggtgtcgcg
     1621 gatcgcgtcc gcctgctcgg ctccgctccc catgccaagc tcgccaaatt catgtgtacc
     1681 gcggacgtct tcgtcctgcc gtccgtgacc aatgccgaga cctttggcat cgctcaactg
     1741 gaagcgatgg cggcgggccg gccgatcatc aatacatcgc ttgacacggc agttccgcgt
     1801 gtggcgcgcc atggcatcga ggcgatcacc gttccgcccg gcaatcatgc ccagttggcg
     1861 gaagcaatcg aaacattgct ccgcgatccg gagcggcgcc ggcgcatggg ccatgccgcc
     1921 cggcttcgcg caacgacgca atactccatc gacgccttcc ggaaaggaat cgaaactgtc
     1981 tatcgccatg ccgtgacgac caaggcatca agccggtcgg ccttggcttc acgccccgga
     2041 acaatcggaa cgggtccgga aaatcgacat tgctgagact gattgccgcc gtctatcccc
     2101 ggacctggcg gccgccgaca atatcaatct gctgtcgcgg atcagtggac gaaaaccgac
     2161 gcccgcagtc gtagacgaga ccgtcaatag ttagtcagga aggaggtact aatgtactcg
     2221 gaaagcatgc ctctcgtaga gaaggagtgg ggcgggcgtc cgctcgatct ggacgcccga
     2281 tccgatcagc gcattggtaa acggcccatg aaaccgtcga tccgttctcc tctcccctcc
     2341 ggcctcaagg acgatcatgt cgcgagcgac cggccgtcga ttggcaggcg aatattccgc
     2401 acccttaccc ggttctctat tgcggttctc atcggcatcg gcgccaccct tgcctggcag
     2461 tcccacggtg acgcagcaag ggacatggta gtagcccggg ctccatcgct ggcctggcta
     2521 ttatccgtct cgacgacgaa gtcgccagcc atggctgcaa cttccccgga cccggcgcag
     2581 cagcttgagc ccctggcgtc caatctcgat gtcgtgcggc gcagcgtaga gcaactcgcc
     2641 gccaagcaag aacagatggc ccagaacatg gcgacactgc aagctgtcgc ggacgacatc
     2701 agacagagga tgtcaataac cccgccttcg tccccgtccc agcagcaggc tgcccccatc
     2761 ccgcagcata aacccccgca acccagggcg cagtcatcag ctgttcagtc atcgtcagtg
     2821 ccgcgtccgc tgcctcctgc cggagcaccc ttgctctcgc gctgatggcc cagattgccg
     2881 ttaaatcggc aggagtattg atgcgccagc caccgcgctc aaataaggat ttcggcgacc
     2941 cccgccctgg aggtctctgc gggcggcccc ttcccctgcg cgtcggggct ccgcgcgggc
     3001 tagacggcac ggagcaacga agtcgactac gaagaggtca gcaatctccc gtcgattgga
     3061 agggaagaat ccgaagggtt tgttgatcag cccggcgaag ttgatcgttc aatactgccc
     3121 ctttggctcc agccccaacg cgtgctggcc gtacatggtg gccaccgcgt cccagttgag
     3181 gctgatatgg gcggcggcgg catgggcgtc gcgccacgcc cgctgcagcg gtttttcgag
     3241 gaagattccc tggccgccgg cggcgaggaa cagcgcgtcg accgcctgca ccagcaggcg
     3301 cgtgcagaag gtgtggccga gccggttgcg cagccgcatg tcgagcggcg tcggctcgcc
     3361 acacgcagcg accgcgtatg tctcggcaag gttatggaac atcatcaagc gtgctgcgtc
     3421 cacacatccg gtggcctcgg cgaggcgcgc ctggatagtg gcaaattcgg ccatccgtcg
     3481 gttgccgccg ccgacggctc cgcgtgttgt gcgtcccgtc accatgtcga cgaaatcggc
     3541 cagcgcgccc tccgccatgc ccagcaccgg cgcgaccagc gttaccggca ccaccgaaag
     3601 gaacgactga cgatagagtg ggttggtgtt gacaagcgtt cccgggctag tcccagcggc
     3661 aaggtccgcg atcgccaagg cgcgatgagc tggaacgaag agatcgtccg ccacgatgtt
     3721 cttgctgccg gtgcctgcga gccccatcgt gtgccggttg tcgtcgatcc tgacatccgc
     3781 gcttggcacg agaagaaagc tcggcctcgt cgcctcggat ccatcgaacg gcgcgaccat
     3841 ggtcgcgaga aaataccacc gcgcattgtc gcagccgctg gcgaaactcc agacaccaga
     3901 agtccgatag cctccgtcaa ccggcactgt cttgccgacc ggtgcatagg acccggccgc
     3961 tatggcacct ggctcggccc agaattcgtc ctgtgcctct ttgggaaggc atgcggcgaa
     4021 ccattgatgc acggcgccca ggccgtagac ccaggcggac gatccgcagc cgcggccgat
     4081 cgtggcgacc aggtcggcga aaacatcgaa gccgtactca aacccgccat aagcctcagg
     4141 ctgcaagatg cggaacagct cggcatcgtg catgctctcg atgaggtcat cggaaatgcg
     4201 gcggttcgcc tcggtctctc gcgcacgagc gcgagcgatt tccgcaatga tacgggcacg
     4261 cgacagcaac ctcggaacgt caggtctggg cggtgatgaa ggattggagt tgaccgacat
     4321 cttcgcgctt ccttcgacgt gcgctcagcc cgatgtcact tctttgccgc cttcatcgga
     4381 tccttgacgg cctcgatggt agcgaattcg acgttgtaga gctgaccatt taccctctcg
     4441 actcggcgga tatactcgtt ctgcacgatg tcgcgcgttt ccgggtcaat ggaaatcgga
     4501 ccacgcgggc tttcccagct catgcctttc atggcagcga ccagggcatt gccatcgaca
     4561 ttgccgctgg tcttcttcag cgcctcgtag atcaggtgca tgccgtcata gccggcgacc
     4621 gaatggtagt tcgggcgcct gccgtaggcc gcttgaaagg cttcgacaaa agtcctgttc
     4681 aattgcgaat cgtgcagcgc cgagtagtga tgggcggtaa tgatgccgag cgcggcgtcg
     4741 gtcatgccag gcagctcgtc gtcgtctgtg agatcgccgg tcccgaccag cctgatgccc
     4801 gacttgtcca tgccgcgctc caggaactgc ttggcgaaga tagccgcctg gttggcggga
     4861 aacacaaaca gcgtatccgg gccaagatca cgggcgcgct gcaggaacgg cgcaaaatca
     4921 ggattggcga gcggcatgcg cagagaatca acgattctgg cattgctcgt ggcgacgcga
     4981 ttcttgaact cggtttcgga ttcgagtccc ggcgcccatt cattgacgat cgtgacgatc
     5041 ttgcgagcgc cgttctgcgc cacccagtct gccatgacgg tcgaggattg gccgagcgtg
     5101 aagctcgttc gcaccatgta gggcgagcgc tcgaccgtga tcgaggctcc tgacaccatg
     5161 acgaccgtgg ggatcttcgc ctcggtgacg agcggcgcaa tggcaagcgc ggacggcgtg
     5221 atgccggcac agacgatcgc aaccctgtcg ttcacgatca tttcctgggc tgtgcgcttc
     5281 gcccgatccg gcgcgctggc gtcgtcactg accacaagtt cgaccctgcg cccggccacc
     5341 acatcaccgt gtcgctgtac gtagaggtgg gcaccgttga cggcggcctt gccaaccgtg
     5401 ctgaacgggc cggtcaatga ttggataagc ccgatcttga gtggctcctg ggcgctggcg
     5461 ccatccacca gagtcattgc cagcgcggaa gcgcaaatca ctgctcgcaa catcgctcct
     5521 cctccggcaa ccgccggcaa aggtcgttcc gctgaaactt ctcttcctcc caacctagcg
     5581 tttggattgc gaccgatacg tgacacactt cacaatcaaa ggactattgt ggcgagggga
     5641 tcatccggtc ccgtcccatt ttgggatcgg gccaggtgcc gtggagcagg atccttcatt
     5701 tagcctgcct tgcatccgcg acgtgcaaat gaaccatatt attaggtgaa acaagctgtt
     5761 tgcccgaagg caatactctg tgaagggaat actctgtagt atccggccga gcccgatgcc
     5821 tcaatcgaag accgagccga tttcggaggg agtcattcag tggacagcga tatcttgcac
     5881 aaactggagc aacgtctcgg accgatccac gccagacagc gattggggat cgagaccgat
     5941 cacgaagcgc aagtctttgg ccagggacgc accttcttcc atctggaaaa cctgccttcg
     6001 gcgccggcga tcattcgaaa tgcgctgaag ctcacgggac tctattggcg ggcttgccgg
     6061 aatgcagagc gcgttatcgt caagcgaaac gagctgaagt tcgctgcatt accatcgctt
     6121 ttcgacggct acactattct ccacatcagc gacctgcatg tggacatgag cgaagccgcc
     6181 atgcagcgcc tgatcgagct tgtcgacggc ttgccatacg atctatgcgt tctaaccgga
     6241 gattatcgag gaaagacttt tggaccgttc gaggcggccc tggcgggcgt ggcaagcgtg
     6301 cgagcccacc tgcggctacc gatctatggc gtgctcggca accacgatac catccagatg
     6361 gttccggcga tggaagccat gggcatacca atgctgctca acgaatgtga agcgatcgcg
     6421 cgcggcgatc agcgcatcta tctggccggg atcgacgacg cccacttctt cagggtcgac
     6481 aacattgaaa aggcggcgtc gcaaatcccg aaagaaggtt tttcgatcct gttatctcat
     6541 accccggaag tctatcgtca ggctgcccat gccaatttca atctgatgct gagcggccac
     6601 acccatggcg gccagctttg tcttcccggc tctatcccga tcaagctgga ggcggtgttg
     6661 ccacgacgaa tgggggcggg agcctggcag taccacaaca tgagcggcta cacttccgtg
     6721 ggtgcaggat cgagcgtcgt tcccgtgcgc ctgaactgtc cgccggaaat caccctgcat
     6781 tgccttcggc gcggctgacg gcgagataat atggtctatc gcgccgccag gctttgaggc
     6841 catccgaatg ccttgcggcg gtctgatttg ccaagtctcg ctcagaaaag tctcgctcag
     6901 aaaagtcttg ctcctaaacc tacgcgggaa cgataacctt gccgatcggc cacagcgcga
     6961 tccctgcgag tttcagatgc gcccaggcga acgggatgcc gatgatggtg acggcgagcg
     7021 cgatcgccgt gacgaggtgc gcgagcgcaa gccaccatcc cgcgagcacc agccagatca
     7081 cgttgccgat cagaccgagc gggccggtgc cgatatcagg gcggcctgtg aggctgtcgc
     7141 ggcgcaccgc cctctgaccg aaaggcaaca aagtatagac ggcgatgttg aaggcggctc
     7201 gcgcccacgg caggcctata atggtgatgg ccatgatgaa ggcagctatc acccaggccg
     7261 ccgccatcca caggccgccg aagacaatcc aaaatacgtt caacaatatc gaaacagggg
     7321 gcatgggatt tctttgaggt tggagcgacg gggctttgcg ctgtggtgcg tgcagcaccc
     7381 ttggctaaac acggacccta tccgctttgc aagccagcgc ggggcggcac gacatccctg
     7441 aacgcgtcga accccttcca ggctggttcg acgggagcga cccctgcatg gggaacataa
     7501 accgacggtt ccgtgagcgt catggaggga atatagcgcg gacagtttgg aaagatcacg
     7561 ttcaccttga tgcgaatgat caattgcgcg ccgatcgtgt cggcaagcaa gggatcatcg
     7621 cgttcgatcc gggcttcgcc gttgatgcgc aatcgcttgg gattgccatg catcgcaatg
     7681 aacagaagtc cgacgtgcgg gttgaccgca aggttgccga cactcttgaa catgccgttg
     7741 ccgtcatagt cgggaaacgc aagttccgac ggtccggtga tgcggacaaa ccccggtgcg
     7801 ccacccttga atgaacaatc cggatgcccg gacgcgtcgg cggtcgccac aaagaagtag
     7861 atgcagcttt cgataaagat cttgtcgtcg tcgctgaaca ccgtgcgtgc cagcttttcc
     7921 tcgagccgat cggcaaggcg ccggctgtca aactggtcct gaaatgcacg attgccttgg
     7981 tgatagagca ggcttgtcat gactggtctc cgtggccgga tcatttgcgg aacagcgcca
     8041 gcaaattggc ccgcatgttc gaattttcgg tcattcctgg ccgggctagc gccctttttc
     8101 ctcgatcgtg cagggtgccg ttggggccgt tccattgttg ggccgcaatt cgggagtcac
     8161 gtattcggtc agaatgcaca atgggaacca tatatgaaaa ggcttgtcct tgggtcggcg
     8221 atattcttga catttgcacc ggcgatagcg ctggcgcagg ggcgtggcag tgatgctgcc
     8281 ttgggtgccg tttcgggcgc ggtggtgttc ggccccgtgg gcgcggtggc aggcgcggtc
     8341 gtcggatata cagctggacc atccatcgct cattcctggg gactgagaag gtccggttcg
     8401 aagcgaatag cccgggagag atcccgcgcc ggcacttctc cgagccgtat cagccaaaat
     8461 ctgccgccga cggcggcaag cccggagcaa tcgggaacca tgggggctgc accacctaag
     8521 cccgcgcccg ccgccgtgcc gcggggtgca gcgccgccgg ttcaaggctt ggaatagttt
     8581 caggaaaaga tatctgcctg ccgacggtag ccagatcacg ttccgatcgg gccggcggcg
     8641 cgccacgatc agtgaggatg tcgcggcgct caagctgaga tctggacctc gccttcaatc
     8701 tccaccggca tcccaaaggg caactcggcc atgcccaccg cgctgcgtgc atgggctccg
     8761 gcctcgggtc caaagagctc aaggatgagg tcggagaagc cgttgatgac ggccggctgc
     8821 tggacaaagc cgggtgcgga attgaccatg cctaaaatgc gcacccaatg cgagatgcgg
     8881 tcgaggtcgc caagcgtacg tttgaggtag cccagcatcg acagcgccgt gagctttgcc
     8941 gccatgtagc cctgctccag cgtcagctcg cgtccgacct tgcccagcgg ttgcgcgaga
     9001 ggtccctcgg gactctgcgg cccgtgcccg gcaatgaccg cgcggtttcc gatcaggcgc
     9061 acgttgacaa agggaaggac aacgccgggg ggcgggctga aggctggcgg caggaccagg
     9121 ccgagagcgg tgaggcgggc ttcgattttg gccatgatga tcttctcaag acgacttgcg
     9181 ggctgcgctg cgcaaccgtc ccaccacccc ggtgggaaag aaataaacgc tggcgatgaa
     9241 aagcagtccg agccacagca gccagcgatc cggatgcagc aatcccggca gcagcggcaa
     9301 tccggcgtcg gcggcggcgt tggaggcagc gcccatcagc gcctgcagat agttctgcgc
     9361 cagaatgaag atggtggcgc cgacgatcgc gccgtagatc gtgcccatgc cgccgatcac
     9421 gaccatcagc aggatatcca gcatgatgga aaagctcagc gaggtgtccg gtccggcata
     9481 acgcagccac aaggcgttca acatgccggc gctggcggca accagcgccg caatgcaatt
     9541 ggcgtaggtc agatgaaaca cggtgcggaa gccgagggcc tcggcgcgga aacggttctc
     9601 ccggatcgcc tgcagcacgc gcccgaacgg cgaattcacc acgcgtaaca gcgccaggat
     9661 catcgccgcg gccgcgacga agataagata atacgtcagc acgcggccat tgagttcgaa
     9721 gccgaagcga tctttcgaga tcagcacggt gcccggccgc aacaattccg gcagttgaaa
     9781 gctgcggccg tcctcgccgc cggtgagcca tgacagttgc gatgccagca ccagaaaggc
     9841 cgatgccacc gccagcgtga tcatcgcaaa gaatatcgcc tcgacccgca acgagaacag
     9901 gccgatggcg agcgcaagca gcatcgccag cggcaggccg acgacgacgc cggtcgcgac
     9961 cgccgcccag ctcggtccca tcgaataaag tgcgatcgcg atcgaatagc tgccgatgcc
    10021 gtaaaacatg gtgtgcgcga acgaaaccga gccggtgtag ccgagcagga ggtcatagga
    10081 cgccaccagg gccgcgaaga tgcagatctt ggtggcgacg ttcatcgcct tggcgccggg
    10141 aaagaccagg ggcgtgacgg ccagtgcggc aatgatgacg acaagcacga tgctgagaac
    10201 gcgactgcgc ggcggatcgc ccgagaggat catcatcggc ttgtcacccc ataaagcccg
    10261 cgcgggcgcc acatcaggac cgcgaccatc aagagaatat tggacaccag cgccagtttc
    10321 ggcaccagaa agcccccgta atttgccacc atggccacca gcagcgcggc cgccgatcga
    10381 gccgaggccg cctatgatga cgacgataaa caccagcacc gtgagatcgt tgcccatcga
    10441 agcatgcacc tgctcacggt acagcgccca catcacgccg ccgaggccgg caagaccgga
    10501 gcctgtcatg aataccccga ggaacagccg ccggatccgg taaccgagcg cttccaccat
    10561 ctcgcggttt tcgacgccgg cgcggatcaa aaggccgatc ttggtccggt tcagcaccag
    10621 ctgaatgcct gcgaacacga tgaggccgac cagcatcgcc agcaggcggt acttcgcgat
    10681 ggcgatgtcg ccgatgatga acgagccgcg caacgatgtc ggcagcggca gcggaatgat
    10741 ctgcgggccc cacagcgcat acagcgcctg ttcggcgacg atcaatccgc cggtcgtcat
    10801 caggatctgc ttgaggtgct ggccgtagac cggcaggatc agcacccgtt cgaccaccag
    10861 gccaagcgcg ccggagaccg ccatcgacaa cacggcggcc ggcgccagca ccgcaagatt
    10921 gagccaaagc gagtcggcct gcacgtaagg cgccagcggc aacagcacca gcgtcgcggc
    10981 ataggcgccg accgagatga aggcgccgtg accgaaattg agcacgtcca tcaggccgaa
    11041 caccagcgtg aggccggaag ccatgatgaa gatcatcatg cccatggcaa ggctcgccac
    11101 cgtcagcgtc acccaggagc ttgccgatcc gatcagcggc agcatcgcca gcgccaaaat
    11161 caccggcgac agcaccggca gaatatcccg cctcggcctg ggcaacgcgg cgggttcggc
    11221 aaggtcggtc actgatgcgt ctccagactt aggcccagca gccgctcctg cagtgtgacg
    11281 tcggtggcca gcgccgccat ctcgccgcgg tgaacgatca agccgttatc catcaccaga
    11341 accctgtcgc cgagctcgcg cgctgcgtgg aaattctgtt cgaccaggag gatggtggcg
    11401 ccgtggcgct tgacttcggc aaagcattcg atcagcgcca ccacgatcgc gggcgccaga
    11461 cccttggtgg gctcatcgat cagcagcagc ttgcgcggtt cgacaatggc gcgggcaatg
    11521 gaaagcatct gcttctgtcc gccggacagg ctgcctgcgc gcgacagcca gaagcgcctg
    11581 agcgcgggaa agaatccgaa aatccactcc aggcggctgt cgtcgagcgg cccgtcccgc
    11641 gccgccagaa cgaggttttc cttcacggtc agatcggaga acaccgccat gctttccggg
    11701 acgtaaccga cgccgagccg cgcgatatcc ggggtcgcac tgtgctcgat ggcttgtccg
    11761 tcgagcgata tccgccccga ggatgcctgc cacaggccca tgatggttcg cagtgtcgtg
    11821 gtcttgccgg cgccgttacg gccaagcagc atggtggtct ggccttcggg caccgcgaaa
    11881 tcgataccgt gcaggatgtg ataccgcccg atatgggtgt gcacgccggc gagggtgaga
    11941 aggtcggtca tgccgcgctc ttgccgggcg cgatgccgag ataggcctcc tgcacgatcg
    12001 gggaagcgat cacatcggcc ggcttgccgt cggccaccag ctggccgtta tgcagcacaa
    12061 tgatgcggtc ggccagcgaa cgcaccacgt ccatcttgtg ctcgaccaac aggatgatct
    12121 tgctggcatc ctgcttgagc tgcgcgatca gattgagcac gacggggact tcatcgatgc
    12181 tcatgccggc ggtcggctcg tcgaacatga agaccttcgg ctcgagcgcc atcatcaggg
    12241 cgacttcgag cttgcgctga tcgccgtgcg acagcgcggt cgccggcacg cgtcgccggc
    12301 cgccaagtgc taccttgtcg aggatcgcgt cggcccgggc aatcaggtcg cggcgcttcg
    12361 tccatggccg cagcatctca taatgcacgc cgctttcgga ttgcactgcg aggcgaacat
    12421 tctcttcgac gctcaaattg ggaaacaaat tggtgagctg gaacgcgcgg ccaaggcctg
    12481 cgcgggtgcg tagcggcgcg gaaagctgcg tgatatcggc gccatcgagc aggatcctgc
    12541 catcgctggg gcgcaactgt ccggagatca gattgaaata ggtggtcttg ccggctccgt
    12601 tgggaccgac aatggccgtc agctccccgg ggcgaaaggt gcagctgacg cggttaaccg
    12661 cgacgtgtcc gccgaagcga atggtcagat cgcgggtttc gagtgtgaag gtcattccag
    12721 cgccatcgag gtctgtgtct tgtcgcgccg gtgttgcggc ggattctgat gtcatgcgcg
    12781 gacatggtcc gcgcatccat cctctttcaa aaatgcattt tgttcggaag aggatggatt
    12841 gccgggtcac ctggcgcgaa gacgcgcttc gcgctgttgc ccggcaatga cgaatacaat
    12901 caccgcttgt tgcggatcgg gatatccatg tcctcgatct tgagttcgcg caccggttcc
    12961 agcacggccc aggccaggtt cggatcgatc ttgaccttga agtgatacat gctctgcagc
    13021 gcctgatggt cttccttgcg gaagatcatc ttgcccttgg gcgtatcgaa ctccaggcct
    13081 tccatcgcgc tgatcagctt ttccgtgtcg gtggatttcg ccttggtgat cgcggccacc
    13141 gccgccatcg cagcggaaaa tccgcccgcg gtgaagaagt ccggcggtgc gttgaagcgc
    13201 ttctgatgct cggcgaccag ccagtcgttc accggatttt tcggaatgtc gtagtagtag
    13261 taggtcgcgc cttccatgcc gggcagccgc ttgtaggcgg ccagcgccgg caggatgttg
    13321 ccgccggtcg aaagctcgat gccgtaacgc tttgggtcca tgtcctgcaa tttggtcagg
    13381 gggtcgccgc cgccggccca gatcacccag atgatcttgt gtcccggctt gtccttcagt
    13441 gcgtcgaaca gccgttgacc ggccgcggtg aaatcggtgg tggtggtcgg cacgtattct
    13501 tcagccgcta gcgtcgcgcc ggtttttgcc agcgcctgct tgaacgcagc tacgccgtcg
    13561 cggccgaagg catagtcctg gccgagcgtc gcgatcgtga cgcccgcttt gccgatcgcc
    13621 accgcgttcg agatcgcgtc ctgggaggaa ttgcgcccgg tgcggaagat atatctgttc
    13681 cacttctcgc cggtaatctg gtccgcgacc gccggctcga cgatcaggat cttctcgttt
    13741 tcctcggcca ccggcaggtc tgccagcgca gcagcggaag acgtcgtgcc gatggcgatg
    13801 tcggccttgt cgtcctgata ggcctccgcg agtgcggcct tggacagatc gggcttgctc
    13861 tgatcgtcct tggtaacgac gacgatcttg cgcccgtcca gcgtcatggt gcctttggtg
    13921 gcatattcca gacccatccg cagaccggtc tcggtctgct tggcataggc ctccagcggt
    13981 ccggtcttgc cgtagatcag ggcgatcttc agatcatccg cctgggcggc cgtcccgagt
    14041 aaagcgacag caatggcctg cacgattagg gtcgttcgca cggctattct cctcccagtt
    14101 tcgatttcgg cgatctgaac gccacagata gtgtgacggc tcgccccggg tcaagccggc
    14161 agtgttgcgc acggttgctt cccggcggcc aatgttccat tgaaccaccg ctccatcagc
    14221 atagtctcgg tggaggtcaa cgcggcggaa gtctgcgaca acagggagag aaacgatgaa
    14281 atatctgtct gccgcgatcg gtctggccat tctgatcgcg acgtcgcatg tcggccgcgc
    14341 cgaggacatc acccgctttg cgccactcaa ggccgaggaa ctgacgccgg cgcaaaaggc
    14401 atgggccgac atgatcgcag ccccgccgcg caatgcgaaa ttcgtcaatc cgccctatcg
    14461 cgcctatatc cgcaaccccg atcttgcgcc ccgcctgtcg gcattgtcgg actatctgcg
    14521 ctggaatacg tcgctgccgc cgcgcctgag cgagcttgcg atattgatca ccgcccggca
    14581 gtggaccgcg caatatgaat ggtttgcgca ttacccgctg gcgctcaaag gcggtcttga
    14641 tccaaaggtc gccgccgata tcgcggccgg caaacgcccc gacaacatga gggacgacga
    14701 ggcggcgctc tatgatctcg ccaccgcact ttatcgcgac aggaaagtct cggacccgat
    14761 ctacaaggcc gcgctggaaa aattcggcga acgcggcatc atggatatca tcggcatcat
    14821 cggctattac gatctggtct cgatgacgct gatcaccatg caggccgagg cgccgaacga
    14881 cagcgtgccg ccgctgcagg ctttatcgaa ataaatgcga gcgtggaccc gcgcgtcagc
    14941 cgcgataacg ctcgttgcgc gcgtaaaccg agcggctttc ctggtgcgga cggctcgccg
    15001 cactggcgcg cgcgtggccc aggatccgaa ccggcggcct cagttcttcg agaaaaatcg
    15061 gcctggagaa atagtagccc tgcgcgtaac ggatcttggt ggcggcctgc agataggcga
    15121 gttcctcgaa ggtttcgatg ccctccgcga cgacggtcat gccgagggct tcgctcagcg
    15181 attcgatcgc ccgcaagatc ccctggctgc gcgggcgctt gtggatgtcg gtgatgaacg
    15241 accggtcgat cttgatttcg tcggccgtga tgtcggccag tgccgacaac gacgaatagc
    15301 cgatgccgaa atcgtcgatc gagaccccaa ctccgagctt gcggaacatc ggcagaattt
    15361 cgtcctggaa atgggtcttg gcaacgaagg cgtcttcggt cacctcgatc atgaagcgct
    15421 tggggtagcc ggtcgcctca agggctaacg caaacgggcg catgaactgc ggattgccgg
    15481 cctgcttggc cgcgacgttg atgctgatgc tggtgtcggg gccgaaggtg tcgttgatca
    15541 gatcaatcga tttgacgatt tccgccagca ccagatgggt aagctcgtcg atcagtccaa
    15601 gctccacggc gagattgacg aatgtgccgg gggcctgaat gaccccgtcg tcatcgcgca
    15661 gccggaccag cgcctcgatg cccttgatct cctgggttcg gatgtcgact ttcgcctgga
    15721 aggcgcagca aaagcgcttt tccagaatgg cgagccgcag cgactgctcg accttcattc
    15781 gcgcaagcgc ctcgcgctcc atgccggcgt cgaaaaaggc cgcggcgccc ttgccgtcgt
    15841 tcttgacgct atacatcgcg atatcggcgt tctggcacaa ctggtcgaag ctgcggccgt
    15901 gctcgggaca aaggccgacc ccgatcgaag cggatgcgaa tatttccgat ccatcgatga
    15961 aaaatggagc ccgcagccgc tgcagcacaa agtcgatgta ttccgtcatc tcgtgctcgc
    16021 cccggatcgg atcaagcagc agcaggaatt cgtcgccgct gatccgcgac agcatgtcgg
    16081 attcgcgcaa attcaacccg atccgctttg cgacttccac cagcagcgcg tccccgatcg
    16141 cgtgcccgta atagtcgttg atctgcttga agttgtcgat gtcgagaaat gcgagggcga
    16201 acctcgccgg cgtttcgtcg cgctgcagaa gaccgttggc gcgatgttca atcacacggc
    16261 gcgccggcag accggtcagt tcgtcatagt aagccgaccg gaacagctga gcctcgatgg
    16321 ctttttgctc actgatatca gcagagctcg aaagcagcag atcgcggtct gcaatacgaa
    16381 ccggccgatg ggccgtgaga agcgtctgtt tgccctcact gccggtcacc gattcctcga
    16441 taacggcagc gcgaccggcg cgcagcaatt ccagaccggt ttcgcggcgg tgggtcagtt
    16501 cctgcgaatc cggcgccgcc tgcccgccga cggcaatccc aagctgcgcc gcggcggcat
    16561 cgttcaccag caggaagcgg ccttgcccgt cctgaaccgt cacgccgccg gggagcaatc
    16621 tgaaaacatc ccgcagaacg ctcagttcgg tagccacggc ggatccgccg ccggcttccg
    16681 tcgcggcggt ccgcgggtca tgtttggcgc ggctatgcat aaccgcgaag tctgccgagg
    16741 tctcttgtag ttttggttaa gtggcagcga caaactcgat gcgacggcgc gtagacagcc
    16801 gacccggatc caaacgccgc gaggcgtcgt taacaatgcg tcaacgggtc ttcctgcctg
    16861 cccgggactg ctcacgccag gggtatgcgg cactgcatgg tgcaatgaac cccggttttc
    16921 aaaaaactga tctcgctctt gccgtcaaaa ggagtgagcg cccctttcag caacttggtt
    16981 ccaaatccgg cctccccgac acttgcgacc ggaggaccct cggtctcgtc ccaggtaatg
    17041 ttgaggcgat cgcctgacac cgaccacgat acctgcaaca gcccgcgcgc cgaagaaaac
    17101 gcgccgtact ttccggcgtt ggtggcgagt tcgtgaaaga tcaatgccag gctcacggcc
    17161 agtttggcgg ggagaaacag cggattaccg ttcagggtaa atcgaacatg cccgtaaggt
    17221 ccgagttcag agagcaagag atctctgatg tcgcaaccgc tgccgtccac ctttgcgatc
    17281 agatcatcgg tcgcggacat cgcccgtaac cgctgatcga tgccggccca gatttgcggt
    17341 tgatcctgca gcactagatg aagaacggcg tgaatggtcg acaatttgtt tttcagccgg
    17401 tgctggaact cgtcgacaat aagcttgcga tattcctcct cccggatcag gcgccctgag
    17461 atctcccgtt gctgcgacac gatcgatcgg tagtgctcga ttccccatat cgtgaggccg
    17521 cagacgatca gataggtcgc cagcagcacc attctggtaa aatcggccgg ggcgttgctg
    17581 aaactgacgg tcgcgccgag cgtgccgccg acgatggctg tcgcgatccc tatccgcaaa
    17641 ccaccgagtg cggtggcgaa gaatacggcc ggaatatacg gggtgaaaaa tatgtccggc
    17701 cggatcaggg caaagcccca ccgcgccgcg gtcgacagag cgaggcaggc ggccgcaaat
    17761 ccaacgttaa aaatcggcgt tggctgggat attccatgcc aaccgcgccg aaattcctcg
    17821 atcaatttca ccatgcgtca caaagctcag gcgatcagcg aatcttgaaa gggcggagta
    17881 taggagaaaa tacgcggcgc gcggctacaa aggcgacaaa cccggcccgg catcggggca
    17941 cgcaaccggc atgggcttgc gttgttccgg cggggtggga atatgacgaa acacaatatt
    18001 actcaaaaac gaggaggatg acatgggacg gctggacggc aaggttgcgg tgatcactgg
    18061 cgcgacaagc ggcatcgggc tgcggaccgc ggagattttc gtcgcggaag gtgcgaagat
    18121 cgtcatcgcc ggacgtcgcg cgccggaagg ccaggcgctg gccggcaaac tcggcgtcaa
    18181 ctgcgtcttc cgccagaccg atgtcacggt tgaagacgag atgcgcgcgc tgatcgggca
    18241 ggcggtcgag aaattcggcc gccttgattg tctgttcaac aacgccggcg gcccggcgca
    18301 gaccggcggc atcgaaggcc tcgaggttgc ccgcttcgac gccgcgatgg cgaccctggt
    18361 gcgcagcgtg atgctcggca tgaagcacgc cgcccctcat atgcgccgcc agggatccgg
    18421 cagcatcatc aacaatggca gcatcgcagg aagactcgcc ggtttctcgt cgtcgatggt
    18481 ctatggggcg gccaaggccg ccgtcattca cctcaccaaa tgcgtggcga tggagctcgg
    18541 cgaatccggc atccgcgtca acagcatttc gccgggcgcg atcgccacgg gtatcttcgg
    18601 caaggcgctc gggctttcgg tcgaggcggc ggagaagacg tccgatgtga tgcgcgaggt
    18661 ctacaagacc gcgcagccga taccgcgcgc cggtctgccc gaagacatcg cccacgccgc
    18721 ggtgttcctc gccagcgacg aatccagttt catcaacggc catgatctgg tggtcgatgg
    18781 cgccatcacc ggcggccgca actggaccca gcaacagcag ggctatgtcg ccttgcgcaa
    18841 ggcgttcgat cagggcgccg gataggggcc gccgtcattc cgggtctggt gccagcacca
    18901 tccggaatga cgaatccggg ttgtatacaa tttgaaccag actgcgggag atcatcatgt
    18961 ccacaacggc cgctatgatg cagacgcgac ctgaagcccg gccactttat gcagccatga
    19021 tcgccgggct ggcgtgcgct ttcgtgatcg ggaaaacgct gccgatcatg atggatacgc
    19081 tttcgatggt gcttgcgccg ctgtgcgcgg tcggcgctcc ctcgggttcg atgcctgatg
    19141 ctatcgaacc gatcagttcg cacgcgctgc ccaatgtgcc gggaaagcgc gtgacgatcg
    19201 tgcgcgtgtt ctacggtccc ggcgggttca cccccgcgca tcgccacgcg ggctccgtca
    19261 ccgcctacat caccaaaggc gagattcgct cgcagcttgc cggcggtccc gtcgagacct
    19321 tcaaggtcgg gcaatcgttc ttcgagccgc ccggcgcaac gcatctggta tcggcgaatg
    19381 cgagcgccac cgaaacagcc gaactgatcg cggtgttcgt ggcggacgag ggcgcgcagc
    19441 tcacgacctt tgtcgagtag cgatgccttg ccgcgcttca agcgtcgggc cgctgatcac
    19501 gtcggtgtat agtcggcggc gacggcaacg tgtgggtaag cggcatgaac gcaaggcttg
    19561 agcgaaaact gcgatctctt gctgccctcg tcgtggccgg gctcatcgcg ggaatcgtgt
    19621 ttaacctcac gcagggccgt acctcgccca cctatttggc ggtgggagcc tcgtatggcc
    19681 tgctgatgtc cgtcacgctc gggagcaccg agctgtttgt cctggacgga ccgatgcgcg
    19741 agtggctcgg tggtctttcg tttgcaacca gtctgatggt gcggagcacg gtctatgtag
    19801 cgattatcac cgtcatccag tggtttcagt tgggtgaggt catctccgga gtgtcccccg
    19861 acacgtccag tcgagctttc tggttcagct ttatctactc cgtggtgctc tcggtcgtca
    19921 tgaatctggc tctcggggta atcaacatca tcggaccacg cgcattcctg aatttcttca
    19981 ttgggcggta ccatacgccg gtggaggaga agcgcttcgt gttgttcgtc gacattgccg
    20041 gatcgacggg gctggccgag cggctcggcg gaatcgccat tcaccgtctt ctcgaccgta
    20101 cgtttcgtct cctcactgtt gcggtcgtgg actatcgcgg cgaggtgctc gattatgtcg
    20161 gcgacgaggt gatcgtgacc tggccggaac gcggcggcgc gatcgattgc cgcccgctgc
    20221 gatgctttgt ggcgatgcgc gatgaattgt cgcgcgcctc gggccaactg gagcgcgaat
    20281 tcggcgcggc gccgcgtatt cgcggtagcc tgcatttcgg gccggtgatc gtcggcgaga
    20341 tcggtgacgt caaacgcgcg atcgttttca acggcgacgt catgaatacc gcagcaaggc
    20401 ttgaagagct cagccggaac gtggagggcg gttttctggc gtcccgcgcg gcgatggagc
    20461 gcttcagctc ggcgccgccg tttgcggttc gcgatctcgg gcgcttgccg attcgcggcc
    20521 gcgccgatgg catcgacgtg gtcggcatcg gtgcgcaacc tttacctgac cttgcctcga
    20581 tctgatccgc ttacgcaacc ggcgacagcg tcacccgcaa tccgtcccgc ggcttcggga
    20641 tcggccacat ctgccaattc ggcgtgtagc ccggctccag cgagacgctg agattttgca
    20701 ggaaatgccg tgcgaagcat ttcgcctgca tattggcaaa atgcagcccg aggcacatat
    20761 gcgcgccgcc gccgaacgga acccaggcga agcgatggcg gccgcgctgc gcctcgtcgg
    20821 taaagcgcat cggatcgaac ctttcgggat cgggccagat ttccggcatg tggtgggaga
    20881 acagcgggtt gacgccgacc atggtgccgg ccgggatagc gtagcccttg aaggtgagat
    20941 cccgtatggt gcggcgcggg atcgagggca ccggcggctt cagccgcatc gcttccttga
    21001 acgccatctc cgacaacggc atggcctcga gattatcgaa tgtcgtcgct tcgccggcgg
    21061 ccacacccag cgccatcact tcatcgcgca gcctggtctg ccactgcgga tgggcggcga
    21121 gcgcacccac aaaagacgac agcgacgagg tcagcgtgtc atgcgcggcc atcatcagga
    21181 aactcatgtg gtcgacgata tcctgcgtcg acagtagcgc gccgtcctcg tgggtggcat
    21241 ggcatagctg cgagaacaag tcttcgccgc cgcctttggc gcgacggatc ggaatctgtt
    21301 ccgagaagta ggccacgatg cgcttgcggc cccggacgcc acgcgccatc gcggtgcccg
    21361 gcaacggttt gcggatcacg gcgacggaag ccgccaccat gtcgacgaag gcgcgcgtga
    21421 tctcgtcgac ctcgggtccg atctcggcgc cgaggaacga ggtggcggcg aggtcgaggg
    21481 tgagctgttt catcgccgga tagaacagca tcggccccgg ttgcgctttc cattgcgcga
    21541 cccgcgccgc gatgccggta tcgagtgcgg cgagatagga tttcatcggc ccggatttga
    21601 aggcgaccga caatgcgcgc cggtgcaggc gatgttcctc gaaatcgagc agcatcaggc
    21661 cacgcggaaa cagccggccg agaatccggc cccagccatg cgtcgaggag aattgcttgg
    21721 cctggtcgaa cagcaccagc tcattggcct cgggcccgag cagcacgaga ctggtctcgc
    21781 cgaagatgtg gctgcggtag accagcccgt atttggcggc ttgccgctcg acctgtcctt
    21841 tggggtcggc cagcacttcg agcgtcttgc cgatcagcgg ccagccctcg tcgccgggaa
    21901 tatgcgtgag cgaattgcgt ttcggcggcg tgaacgtgat cgcgggggaa gccgcacttt
    21961 gcatcgacat ggcaattgct ccggttggtc cccgacaggc gaggccgatg ccagtaaaat
    22021 aaccctttgc ccatcccgcg caaagcctca agcagcgcgg gccggggtcc ggccaatcca
    22081 aatcgcgcca caaagccgcg cgccgtcggc aagcaatctg aactcaatct cggcgcggcc
    22141 gacaagcatc atccaaatgg ctgagggatc aggtggatgg cgctcctcgc tacgccttct
    22201 gtttcgcggc ttccttctgc agatccggcg cgttgatggt cgggatcgcg acgcggccag
    22261 ggccggtgag cggaagcgct gccgcagcct tgtcgttttc catgatcttc gccatcacgg
    22321 cctggccggc gcgttcgaac accgcgcggt tctcgatcac gaatttctgc gatttgcgca
    22381 agccgtcgag atagccgttg atttccggca ccacgtagcg cgccaccatg tcccagctgc
    22441 ggcgggtatt ttcggggttg gcccagtcgt gcacgaaacc gacgatggtg ccgacgccgc
    22501 cagagacatc catcaggctc ttgatggttt tcaccagatc gtccggcgta ccgatggtgg
    22561 aggccgccgc ttcgccgccg gcggtcttct ccacggcatc ctcgggcgaa gtgaaccgct
    22621 gcaggcccgg ccgctgcagc gtgccgacat tatactcgtt atgccagcgc atcagtccgg
    22681 cgccggcctc gcgctgcgcc tgctcgcggg tctcggcgat gtgccagctc aacaagacgc
    22741 gccagtcgga ccggctcacc gtggtgccgt gcttcctggc ggcatcctcg gcaaaacccc
    22801 attgagtcgg cagcgccatc aggccttgcg tcgacatcga gccgagcgaa atgatgccga
    22861 tgccgtattt gccggccagc gtcatccccg acggcgaaat ctgcgacgcc accacgaacg
    22921 gcatctcttc ctgcagcggc aggatctgca gcgccgcgtc attcatggtg aaccagtcgc
    22981 tcctggcggt gacgcgttcg ccgttgaaca gccggcgaat gatcgcgatc gcctcgtcct
    23041 ggcggtcgcg ctgcgtcatc ggatcgatgc ccagcgtatg cgcgtcggag gccagtgcac
    23101 ccggtccgga accgaagatg gcgcggccac cggtcatgtg gtcgagctgc accatgcgct
    23161 gggcgacgtt gaaggggtgg tgatagggca gcgacaccac gccggtgccg agcttgatgc
    23221 gtttggtgcg ctcgccggcc gcggccaaaa acatttccgg cgacgcgatg gtttcccagc
    23281 ccgaggagtg atgctcgccg caccagaact cgtcgtaacc gagcgcgtcg agctgctcga
    23341 ccagatcgag gtcgcggcgg aattgcagca tcggatgctc gccgatcgga tgatgcgggg
    23401 cgaggaaggc tccgaatttc aggcgcgcca tgagttttct ctccgaacgg cattgttgtt
    23461 gctttactga tgccaaacta cgggtcctgc cgccgcaagg caaatcgctt tcggtcacga
    23521 gtagctcagg gatcgagtgt ctgccatgcg cgcagccgcc gctggcgcgt gcaacagctg
    23581 gagcccggcg gcatgtcttg cacggcatgt cttgccgctt gacagttcaa caccttgtcc
    23641 taggctggat gcgatcaacg acggcgagcg cagagaacat ccgttatcag accagggatt
    23701 tcggttttcg aggtctcgac cgctgacctg cgctcacaaa aaaacaacaa tccggattga
    23761 ttaatccgga ggcatcgggg ggacatgtca tggtgcaccg caccgacatc gccggcactg
    23821 tcgggtgcag cagggggcgg gtagaccacg gcgatgacgg cagctgccgc catgcaccga
    23881 gtggcgccct cgtcatggtg ctttcatcga aagaagtcat ttatcggata cctgccactc
    23941 cgtacttcga cggcgagaag tgcagccgtg tcttcctgac ggcacttccg tcggtccatg
    24001 cgatctatgt cgagaccgtt ggcccgatga ggcgttgaca tcagaacatt gcaaaggtcg
    24061 cgacgacaac gcgacccaac aaaggtacag cacaggagga tacagatgac ttttcatgga
    24121 cgatatctcg gaggctgcgc cgcgctggcg gtggctgcca tattgacggc ctcgccaacg
    24181 cacgcccagg acgctgccat gctcaatgcc gaggcggccc agaacgactg gtcgacgcat
    24241 cacggcacct ataagtccta ccattacagc ggcctcgagc agatcaatgc cgacaacgtc
    24301 aagaatctcg aagtcgcgtg gacgcattat ccggagcgcg ccacccgcgg cctgcagtcg
    24361 acgccgctcg cgatggatgg cgtgctgtac tattcgggct cctacagccg ggtctacgcg
    24421 ctggacggcg cgaccggcaa gacgatctgg acctatgccc cggaactcga cgaggaactg
    24481 gtcgccaagc agacgcactc gccgtacaac cgcggcatcg cactcggcca gggcaatctt
    24541 tatgtcggca cggtcgacgg gcgcctgatt gcgctcaacc agaagaccgg caagccggtg
    24601 tgggatctga agctcctgga ttccaagaag gtgacggtcg gctttaccgg cgcgccgctg
    24661 gtggtcaagg acatggtgat cattggcgcc cagggcggcg aatggcccgg gcgcggaccg
    24721 atcttcggcg tcgatgccaa gaccggacag aagaaatggc agttcgacac ggtcgccggc
    24781 accgaagagg ccatggcgac ctggggcaac gactcctgga ggaccggcgg tggcggcggc
    24841 tggatgcccg gcgcctacga cccggagacc aattcggtgt ggtggggcac cgccaatccg
    24901 gcgccgcttt acgactggtc gggcgccgac tggaagaaga gcggaccgcg gccgggcgac
    24961 aatctctata ccacctcggt catcgcgctc gatcccgata ccggcaagct caaattctac
    25021 caccaggagc tgccgcacga tgcctgggat ttcgactccg cggtgggcga gttcgtcatg
    25081 atcaaccgtg gcgacaagaa gctcgtggtg cacgccaata agagcggatt cgtcttcgtc
    25141 tatgatcgct ccaacgccaa gatcgagaac gtctggccgc tggtcaagaa catcaacttc
    25201 gtcaagagta tcgatccgaa gaccggcgaa ctgatcggtc gccgcgacct cgtggctggc
    25261 aaggcggaaa ccccgttgtg tccggcgatc gccggcggca tcagctggaa ttccggcgcc
    25321 tacagcccga agaccgggct ctactacaag atcggccagg agtggtgcat ggagcttacg
    25381 gtgctgaaga ccacgccgat cctcgagccg atggcccagc tcaacatcgg cgccgacttc
    25441 aagctggtgg cgccgccgga cggggccgcc cgtggtcatc ttgatgcccg cgatccggtc
    25501 accggcgtct tgaagtggga ggtcaactac aagcagccgc cgctcgccag cgtcctcgcc
    25561 accgcgggca atctggtgtt cgtgccggat gccgaaggtg tcgtgcacgc ctacagtgcg
    25621 gatagcggca aggaactctg gtcgcacaac aacggcatgg gccataatgc cggcattatc
    25681 agctacatgg caggtggcaa gcagtacatc gcggtaccgg cgggctgggg cagcctggtg
    25741 gccgacgagt tcgtcgcgct ctatggcgag cccttcaaga gcatggcgaa gaataccggc
    25801 gccctggttg tcttcgcgct gaaataacaa tgacgacggc gttggcggca gggatttttc
    25861 cttgccgcct tggccggtca acacggatgc cggaatgcga atcctgtttg tgattgttgc
    25921 ttccctggcc atcatctggc catggctgcc gaccactgcc gctgccgaac agtcgacctc
    25981 ggcgggatct gcaagtccgc cggcgcaacc cgaggcctcg tcaagcgaca tcgatggcga
    26041 gcaaatgttc gccaccacct gcggcttttg ccaccaggac ggcggccgtg ccgccggcag
    26101 gggtccgaaa ttgtcgaaga ccgagcgcag cgacgaattc atcatcgagc gcatcaagaa
    26161 tggcaagatg ggcgccatgc ccgcgtttgg ttcggtgttc agcgacggcc agatcatcgc
    26221 gatcctggcc tatattcggg ggctcgatga ttagcgcagg tgcgaaacga catcgcgaac
    26281 gccttggcga acgccttggc gaacgccttg gcgaacgact tggcgatgtc aggaccgcgg
    26341 cgtggaagcc gcgatacaac ctcgtccgat acgcagccag catctgcctg ctggcgctcg
    26401 gcagtgccac ggcagatgcc cgctcgctcg aaatcatcac cgagcgcggg gcgttgacgc
    26461 tctgcgccaa ccccaacgcg ctgccgttcg ccagcaagac gggaccgctg ccgggatttc
    26521 agatcgagct cggcgagaag attgcgcaac aactcggtgt caagctgacg cgggagtggg
    26581 tgatcagcgc gatccagtat cgccgcgccg actgcgacct cgtgctcgac accatcgccc
    26641 gcaaggacac cccgcccgcg ggcggcgtgc aggtatcgcg tccctaccat cgcagcggcg
    26701 tcgtgctcgc ggtccgcgcc gactcgcagg cgtcgtcgct ggccagtctc ggcgccgatc
    26761 agcgggtcgg ggtgccggtc ggctcgctgg tctcgatgac gctgggcaag ggcggcaccg
    26821 tcacgtcgcc gttcgtgttc gaggacgata tcgttgcagc actggccaat cgcgagatcg
    26881 aggcggccgc ggtcacgccg gtgacggtcg gctggttcaa cctgcagcat gccgacaagc
    26941 cattgcgcct gattccggcg ttcgacaacg accaggattt gaactggaac atagcggccg
    27001 ggctgttccg ccccgacgac aaactgcgag cgcgtgtcga tgcggcgatc gaggcactgc
    27061 tggccgatgg caccatcgcg cggatctatg gccgctacgg catcgagctg cgaccgccgc
    27121 agtgaggtgc gccgtgaacc tggggtgggt ctacgccgca tcgtgccatg cgcgcagctg
    27181 ccggatagcc ggggctgtca ggctgtaggc cgccaccgtg gtctcggaaa atcgcgtggc
    27241 ggtttcgggc gcgtgcagca gcctcgcgac caccacgccc aacggaacag cgcaccacgg
    27301 atcggtcagc agcggcacca gttcctcgcg cgagccgcga tatcgcccca aaccaagggc
    27361 gagcaaaccg atctcgcgca ggatgtcgcg cctgcggttg cggcgccgcg cggcatcaag
    27421 caatcccatc aatccaagcc gggtgaggac accctcgcgg ccgtcgggtt ggaccggcgg
    27481 cgtctcgccg agcgagaccc gcaacaaggc gccgggcaaa tcgacgccgc tcaaccacgc
    27541 attcatcggc tcgaccaggc ggggattggc gtcgatgaaa tgcggcgtcc tggttgcgtc
    27601 gtcctggatg tagtcgaacg acagcgcgcc gtgccaccgc agcgcctgtc cgatgcgttc
    27661 gacggtgtcg cgaaccccgg cgctgacgac gctgatcttg agcacatcgc cgccgcccgg
    27721 tccttccacc acctggcgat agatatggct ggcgacgaga cggccgcgat cgaacacgcc
    27781 ttg
//
LOCUS       DATNOI010000321         2476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1945153,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000321 DATNOI010000000
VERSION     DATNOI010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1945153"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VN968_12620"
     CDS             <1..673
                     /locus_tag="VN968_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXL30118.1"
                     /translation="ILDDADLKVAVECAANSAFFSTGQRCTAASRLIVTKGIHDKFVA
                     ALTERLSGLKIDDALKAGTDIGPVVDQSQLDQDLKYLAIGKEEGAKLAFGGERLKREA
                     PGFYLQPALFTDVTNKMRIAREEIFGPVAAVIRVNDYAEALVTANDTEFGLSAGICTT
                     SLKYASDFKRNAEAGMVMVNLPIAGVDYHVPFGGRKGSSFGPREQGKYAAEFYTTVKT
                     AYTMP"
     gene            complement(817..>2476)
                     /locus_tag="VN968_12625"
     CDS             complement(817..>2476)
                     /locus_tag="VN968_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXL30119.1"
                     /translation="DHRLEAWSADIKGSEYFTIRVRDWMTREDAADLVEETDGGVVWN
                     ADATAFYYVKLDDKHRPMQVFRHRLGTPQAADVLVYEERDSGWFTHIHESASGRFCVI
                     AGGDHETSEQRLIDLADEQAKPRLVAARETGVQYSVADRGDELFILTNADGAIDFKIV
                     TAPLRSPERANWSDLIPYREGVYIIDIELYAGHLVRLERANALPSIVIRDLKTSDEHA
                     IAFDETAYSLDTMGGYEFDTTNLRFSYSSMTTPSEVYDYDMASRARILRKRQEIPSGH
                     NPAEYVTTRIMATSHDGAEVPVSILHARDLVRDGAAPLLLYGYGSYGMAMPASFSANR
                     LSLVDRGFVYAIAHIRGGADKGWGWYLDGKRGKKTNSFDDFAAAGRALIDAKYTSAKH
                     IVGHGGSAGGMLMGAVANRAGELFAGIVAEVPFVDVLNTMLDDTLPLTPPEWPEWGNP
                     IESAADFKTILSYSPYDNVAARNYPAILAMGGLTDPRVTYWEPAKWIARLRATMTGGG
                     PVVLRTNMGAGHGGASGRFHRLDEVAIAYAFALWAVGMADKGEV"
BASE COUNT          460 a          795 c          775 g          446 t
ORIGIN      
        1 catcctcgac gatgccgatc tgaaggtcgc ggtggaatgc gccgccaaca gcgcgttctt
       61 ctccaccggc cagcgctgca ccgcggcctc gcggctgatc gtcaccaagg gcattcacga
      121 taaattcgtc gccgcgctca ccgaacgcct gagcggcctc aagatcgacg acgccctgaa
      181 ggccggcacc gatatcggcc ccgtggtcga tcagagccag ctcgatcagg acctgaaata
      241 tctcgcgatc ggcaaggagg agggagcgaa acttgccttc ggcggcgagc gccttaagcg
      301 cgaggcaccg ggcttctatt tgcaaccggc gctgttcacg gacgtgacca acaagatgcg
      361 gatcgcgcgc gaggagatct ttgggccggt cgcggcggtg attcgcgtca acgactatgc
      421 ggaagcatta gtcaccgcga acgacaccga gttcggcctg tcggccggca tctgcaccac
      481 cagcctgaaa tacgccagcg acttcaagcg taacgcggag gccggcatgg tgatggtcaa
      541 tttgcccatc gccggtgttg actatcacgt accgttcggc ggccgaaagg gctccagctt
      601 cggcccgcgc gagcaaggaa agtacgccgc ggaattctat acgaccgtca agaccgcgta
      661 cacgatgcca tagcaatcgc cggagtgcgc cttcacaccg ccggcaaccc gcccgttcct
      721 cgccgtcgat gccggcgcca ctatatggcg agctgccact ctatctgcgc gcttaacctc
      781 tccccgcaag tggccgcaag tggggagagg agcaggtcac acctcgccct tgtcggccat
      841 tcccaccgcc cacaaggcaa aagcataggc gatcgcgacc tcgtcgaggc ggtggaagcg
      901 gcccgaggcg ccgccatggc cggcgcccat attggtgcgc aacacgaccg ggccgccgcc
      961 agtcatggtc gcgcgcagcc gcgcgatcca tttcgccggc tcccaatagg tgacgcgcgg
     1021 atcggtcagc ccgcccattg ccaggatggc gggataattt ctcgccgcga cattgtcgta
     1081 gggcgagtac gacaagatgg tcttgaagtc ggcggcgctt tcgatcggat tgccccactc
     1141 cggccattcc ggcggcgtca gcggcagcgt gtcgtcgagc atggtgttga gcacgtcgac
     1201 gaacggcact tcggccacga taccggcgaa caattcgccg gcgcgattgg cgaccgcgcc
     1261 catcagcatg ccgccggcgc tgccgccatg gccgacgatg tgctttgcgc tggtatattt
     1321 ggcatcgatc agcgcgcggc cggccgcggc gaaatcgtca aagctgttgg tcttcttccc
     1381 gcgcttgccg tcgagatacc agccccagcc cttgtcggcg ccgccgcgga tatgcgcgat
     1441 tgcgtagaca aagccgcggt cgaccagcga cagccggttg gcggaaaacg acgccggcat
     1501 cgccatgccg taggaaccat agccatagag cagcagcggt gctgcgccgt cgcgcaccag
     1561 atccctggcg tgaagtatgg ataccggaac ctcagcgcca tcgtgcgagg tcgccatgat
     1621 gcgggtggtg acgtactcgg cgggattgtg gccggaagga atttcctgac gcttgcgcaa
     1681 gatccgcgcg cggctcgcca tgtcgtaatc gtagacctcc gacggcgtcg tcatcgacga
     1741 atacgagaac cgcaaattcg tggtgtcgaa ttcatagccg cccatggtgt cgagcgaata
     1801 cgccgtctcg tcgaaggcga tggcgtgctc gtcactggtt ttgagatcgc ggatcacgat
     1861 cgagggcaag gcgttggcgc gctccagccg cactaggtgg ccggcgtaaa gctcgatatc
     1921 gatgatatag acgccttcgc ggtacgggat caggtcgctc cagttcgcgc gctcgggcga
     1981 cctgagcggc gcggtgacga tcttgaaatc gatcgcgcca tcggcgttgg tgagaatgaa
     2041 taattcgtcg ccgcggtcgg cgaccgagta ttgcacgccg gtttcgcgcg ccgccaccag
     2101 ccgcggcttt gcttgctcat cggcaagatc gatcaatcgc tgctcggacg tttcgtggtc
     2161 gccgccggcg atcacgcaaa atcttccgct ggcgctttcg tggatatgag tgaaccagcc
     2221 ggaatcccgt tcctcgtaga ccagaacatc ggccgcctgc ggcgtgccga ggcgatgccg
     2281 gaaaacctgc atcgggcgat gtttgtcgtc gagcttcaca taatagaatg ccgtcgcgtc
     2341 cgcgttccag accacgccgc catcggtttc ctcgaccagg tcggcggcgt cttcgcgcgt
     2401 catccagtcg cggacgcgga tcgtgaaata ttccgagccc ttgatatcgg cgctccaggc
     2461 ttccagccgg tgatcc
//
LOCUS       DATNOI010000322        11572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1076874,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000322 DATNOI010000000
VERSION     DATNOI010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11572
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076874"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VN968_12630"
     CDS             <1..211
                     /locus_tag="VN968_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL30120.1"
                     /translation="DQRELLPRIKSPTLVIAGRHDVATPVAAGEYIRSQIPGASLTIL
                     DAAHISNVEQPHAFTDAVVGFLTQR"
     gene            237..629
                     /locus_tag="VN968_12635"
     CDS             237..629
                     /locus_tag="VN968_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXL30121.1"
                     /translation="MDDQQRRDAGMAQRRKVLGNAWVDKSAANTNSFNADFIDLITRH
                     AWADIWTRPHFDERTRRVLVIGTLLGLCQWDEFRLHVRAALTEGGFTPDDVKEIILQQ
                     AIYCGVPAANHAVKEASAIIQELGLLKG"
     gene            complement(591..1118)
                     /locus_tag="VN968_12640"
     CDS             complement(591..1118)
                     /locus_tag="VN968_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30122.1"
                     /translation="MSTNWRISSFNGALLAAYFIPTWTIVAFRIVISPIQGLYERPNV
                     SVALFISDHLQLSAMTTVRFAWLLAFSKLTVVAFFVIFLVFIARASIRKAGGCDEALA
                     MALAIGSVISFVSMLLASQVGESAAVKLHATELLLLLGTAIVLLLERPADPQTDAGAA
                     SDDLSLQQSQLLNNR"
     gene            complement(1290..3383)
                     /locus_tag="VN968_12645"
     CDS             complement(1290..3383)
                     /locus_tag="VN968_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL30123.1"
                     /translation="MLNRLSASALLKAVIGLMAACVVAVLSVTGWTSWQRLQTAGRIT
                     VIAEASASAFKAMHNLRNDRPSTSRLLTSDQVIQPDMASYLRTIRGDEMPAMRATIEL
                     LPAIDFADKETLIPALSRLVQTMTALHAESWEAMSQPKASRRPALLKEYMETAEVLLQ
                     TLETVSARLAAAVNHNDPVIDQLLSIKQAAWLFRSMSGEASLLLASALPVGRMTPEAR
                     QSYTKFVGGIEAAWAALELAAAGSKLPSGLVDALATAKTASFDPQYLALRDRIANALV
                     AGEKAEINASQWSPITVGRMATAVTVAESALEQAEIRASAQWSAAREQLVLQLSLLAG
                     ALALALGSMMTVSHRVINPLHAIRDAMLKVAAGDLSVDAGYAERRDEIGALAGALGVF
                     KQQAIEKARIEQQERDRNLGAAARQRAIEAHIAAFEAQIRQALNALDTASDQMRTTSD
                     DMSMVSSQTNAQVHQAARASGEASNNVQNVASASEELSASISDISRQVTHAAGIASRA
                     VDMARQTDDTVQGLSKTASRIGEVVGLINEIASQTNLLALNATIEAARAGDAGKGFAV
                     VASEVKSLAGQTAKATEEISEQIAAVQKVAMEAIQAIKGIGGIIGEVNEVASAIAAAV
                     EQQGAATLEITRSTQQAAQGTKAVSDNIEGVSVGADAAGAAALNVKSAAGVLGSQAQQ
                     LRNQVDEFLGKIRVA"
     gene            complement(3486..4958)
                     /locus_tag="VN968_12650"
     CDS             complement(3486..4958)
                     /locus_tag="VN968_12650"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL30124.1"
                     /translation="MRDAISGRRLVSPTATSSQIPSWPRETIRRSLAEPLAPRNNSKK
                     FEEKTVSKTEDAFDVPAFINSRPIGAVQYGIVVLCGLVMFLDGLDTQAISYMAPMISK
                     EWGLSREVLGPIFSSALTGLMFGYLVLSPLSDRFGHRRLILFSTVAFGLLTLVTLVAT
                     NVTELIVLRFLTGIALGAAIPSTVALTTEYMPRRLRATFVLAIYCGFSLGFVAAGGLA
                     ASMIPLYGWRSMLWIGAVTPLALAVLVYVFLPESLDFLVRTRAKPHGIWRIVRRLDRT
                     LPSAGPQQFLTEVEAKRSAVGSLFQSGRTLGTLVLWLVFGLNLAEFYALQSWLPTILA
                     GHGFSPNAVALATSMTTIGGIVAAFAIGPAMDKLGAYRSLAVVYFAGVVFVALMGPAI
                     SAPAGVLMVAAFCAGFCISGGQKSVIALAAIFYPAPIRSTGVGWALGVGRLGGIGGPL
                     LIGLLLAYELSAASLFYAAAVPMLLAGILVALLGVRYRAS"
     gene            complement(4858..5580)
                     /locus_tag="VN968_12655"
     CDS             complement(4858..5580)
                     /locus_tag="VN968_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HXL30125.1"
                     /translation="MTAKVYRIHRPVEDVAILHLRFPAGIRAKFLAGQYLRINMPDGD
                     TRNFSMANPPHESDGVQLHIRQVPGGRFSEGVLAGLRQGDTLDVELPYGDFHLRPDSD
                     KPVVCLATGTGFAPLKSIVEDLIKRGNKRPVHLYWGGRRQRDLYLADLPRKWAARAGW
                     FKFIPVLSEPHPDWNGAVGLVHHAVLRDLTDLSGWEVYACGNPLMIRHARRDFRTTAG
                     LSDGDFFADPFVASGNDPAVPG"
     gene            complement(5656..5904)
                     /locus_tag="VN968_12660"
                     /pseudo
     CDS             complement(5656..5904)
                     /locus_tag="VN968_12660"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
     gene            complement(5865..6860)
                     /locus_tag="VN968_12665"
     CDS             complement(5865..6860)
                     /locus_tag="VN968_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXL30126.1"
                     /translation="MTPPAIALTIGDPNGIGPEIAIKAAAKLAREGGAAVVLIGDEFV
                     VRSYAESCAKDFSIRKFDGSTARAGALQFLAVDALSREDFRPGTPLAAGGRATVDYVA
                     AAMRFVSEGHAAAIVACPHSETNVNAAGIPFSGYPGLLARLMKVPEDEVFLMLVGGGL
                     RIVHVTLHERLHAALARITPELVERAARTAEAALQRLQIKRPRIGLFGINPHAGEGGL
                     FGDDDDRITVPAARRLRESGLDVEGPLGADLMLGRQGFDAFVAMYHDQGHIPVKLLAG
                     RNSAALSIGAGLLFSSVGHGSAFDIAGRGVAEPEAVLRSIQLVCGASRLDDAATA"
     gene            complement(6857..7327)
                     /locus_tag="VN968_12670"
     CDS             complement(6857..7327)
                     /locus_tag="VN968_12670"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HXL30127.1"
                     /translation="MIDNVYQMIARAQAEYARCIDDDRLEEWPNHFHGQCIYRVTTAA
                     NHREGLEAGLIFANSRGMLADRVSALREANIYERQAYRHILGQPSILFEQNDEVRSET
                     PFLVTRILQDGDTDIFATGRYLDLYKIEDGQVKLNERTVVCDSSRIDTLLALPL"
     gene            complement(7344..8597)
                     /locus_tag="VN968_12675"
     CDS             complement(7344..8597)
                     /locus_tag="VN968_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXL30128.1"
                     /translation="MAQHQEIPNGGQAWPQEGLTRVPYRVFQTEEAYKSEQKLIFQGP
                     SWHYLALEVEVPEPGDFRTTKVGDTPVIVTRDADGEIYAFENRCAHRGALICLDNHGK
                     GKKDFSCVYHAWTYDRQGNLKGVAFKDGVKGKGGMPDSFRMEDHGPRKLRIATLNGLI
                     FGSFSEDVAPIEEYLGEEISSRIERVLGGRTPVVIGRITQVLPNNWKLYMENVKDSYH
                     ASILHLFFTTFELNKLSQKGAIIVDESGGHHVSYSAIDREARKDAEYAKQQLRSESQY
                     KLADPSLLQGFDEYHDGVTLQILSVFPTFVLQQIQNAIAVRQIVPRAIDKTDLNWTYI
                     GFADDTAEQRLTRVKQGNLVGPAGYISMEDGCVGGFVQRGIAGASDQEAVLEMGGGEA
                     RSSESRVTEASVRGFWKAYRSNMGV"
     gene            8721..9506
                     /locus_tag="VN968_12680"
     CDS             8721..9506
                     /locus_tag="VN968_12680"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HXL30129.1"
                     /translation="MPQNTERPARKAEGMGGLAKGLAIVEAFSAHPVMSVADAARAAG
                     ATRASARRCLITLAELGYVEHSGREFRPLTRLRRLGGVASRRDQFAQLAQPLLARARD
                     ALAESVSLAVLDDDGALFIARAEAEHIVSTGVRVGARLPAYCSATGRVLLSQFPDQEI
                     LSRIGRKPLARRTSHTLTKPSEVLAEVRSVREKGYAVSDEELELGLRALAVPVTGANH
                     EIIGAVSVSAASARVRSAELRRRFVPVLRSFAAMLAEAFQSVD"
     gene            complement(9734..9901)
                     /locus_tag="VN968_12685"
     CDS             complement(9734..9901)
                     /locus_tag="VN968_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30130.1"
                     /translation="MSNQNPNPGQQNQNPGQKPGQKPGQQQGGGQKPGQQQQDPNRPG
                     QKPNPGQQTER"
     gene            10182..11096
                     /locus_tag="VN968_12690"
     CDS             10182..11096
                     /locus_tag="VN968_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663086.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HXL30131.1"
                     /translation="MDYFAQQLINGIVLGSIYGLIAIGYTMVYGIVGMINFAHGDIFM
                     IGGFIALISFLILVSIGLTVVPVILLIVLLVSMAITALYGWTVERIAYRPLRHSFRLA
                     PMLSAIGMSFVLTNYSQIAQGARVKPVPPIITGGYTLFDRDGFIVRLSNVQIIVVVTT
                     IVLLAIFTWLVARTRLGRDMRACEQDQTMAALLGVDVDRTISMTFVIGAALAAVAGMM
                     YLLYYGLVDFFMGFVAGIKAFTAAVLGGIGSLPGAMLGGLLIGLIETLWSAYFSVEYK
                     DVAAFSILIIVLIFLPTGLLGRPEVEKV"
     gene            11108..>11572
                     /locus_tag="VN968_12695"
     CDS             11108..>11572
                     /locus_tag="VN968_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3382 domain-containing protein"
                     /protein_id="HXL30132.1"
                     /translation="MTAPVPEPTLAPRAPDVAFIFKKALISALVALVLFSLMIGIRTE
                     AGPAGQLIYWTRFGDLAAVVAAVFGGSIIVEMLRLWWGPVGTIRIVPPSVQSTLAVAR
                     RAIAPVLLIFTLLVPVIFYDQRYILDLGILVLTYVMLGWGLNVVVGLAGLLDL"
BASE COUNT         2140 a         3691 c         3526 g         2215 t
ORIGIN      
        1 cgaccagcgc gagctcttgc ccaggatcaa aagcccgacg ctggtgatcg ccggccgcca
       61 cgacgtggcg acgccggtcg cggccggcga gtacatccgc agccagattc ccggcgccag
      121 cctcaccatt ctcgacgccg cgcatatttc caatgtcgaa cagccgcacg cttttaccga
      181 cgccgtggtc ggtttcctga cgcaacgcta gtcacaacga caatcggggg gagtacatgg
      241 acgatcaaca acgccgcgac gccggcatgg cgcagcgccg caaggtgctc ggcaatgcct
      301 gggtcgacaa atccgccgcc aacaccaatt ccttcaacgc cgacttcatc gatctaatca
      361 cgcgccatgc gtgggcggac atctggaccc gaccgcattt cgacgagcgc acccgccgcg
      421 tgctggtgat cggaaccctg cttgggcttt gtcaatggga tgaatttcgc ctgcacgttc
      481 gcgcggcgct gacggagggc ggtttcacgc ccgatgacgt caaggagatc atcctgcagc
      541 aggcgatcta ttgcggcgtg cccgctgcca accacgccgt caaggaggcc tcagcgatta
      601 ttcaggagtt gggactgctg aagggataga tcgtcggatg ccgcgccggc atcggtctgc
      661 gggtctgccg gacgttcgag cagcagcacg atcgcggtgc cgagcagcag caacagttcc
      721 gtcgcatgca gcttgacggc cgcgctctcg ccgacctgcg atgcgagcag catgctgaca
      781 aagctaatga cgctgccgat cgccagcgcc atggccagcg cctcgtcgca gccgccggcc
      841 ttgcggatcg aggcgcgggc aatgaagacc aggaaaataa caaaaaacgc gaccaccgtg
      901 agcttgctga atgccagcag ccacgcgaag cggaccgtgg tcatcgccga cagttgcaga
      961 tgatcgctga tgaacagggc gaccgataca ttgggccgct cgtagaggcc ctggatcggc
     1021 gaaatcacga ttctgaaggc gacgatggtc caggtcggga taaagtaggc cgccagcagt
     1081 gcgccattga atgagctgat tcgccagttc gtggacatgt cgcttcccgc ttgtccgctg
     1141 tgctccgaat gcgagcggcg gcttcgcggg cgagctaact aggttaaatt gcaaggggca
     1201 atttaaacct tttgtttacc ttaacggcgg gacgaccgtg ggaaatagcc cgaattcgac
     1261 cggggccccc tctcccgcca aaaccgcgat caggcaacgc gtatctttcc aaggaattcg
     1321 tcgacctggt tgcgcaattg ctgggcctgg gaacccaaca cgccggcggc ggatttcaca
     1381 ttcagcgccg cggcgccggc ggcatccgca ccgacgctga cgccctctat gttgtcggaa
     1441 acggccttgg tgccctgggc cgcctgctgc gtgctgcggg taatttccag ggtcgctgcg
     1501 ccctgctgtt cgaccgcagc ggcaatcgcg gaggcaactt cgttcacctc gccgatgatt
     1561 ccgccgattc ccttgatggc ctggatggcc tccatggcaa ccttctgaac agccgcgatt
     1621 tgttccgata tttcttcggt cgcctttgcg gtctggccgg cgagcgattt gacctcggat
     1681 gcaaccacgg caaatccctt gccggcgtcg cccgcgcgcg cggcctcgat ggttgcattc
     1741 aatgccagaa ggtttgtttg cgaggcgatt tcgttgatca ggccaaccac ttcgccgatt
     1801 cggcttgccg ttttggaaag tccctgaacg gtgtcgtcgg tctgccgtgc catatcgacg
     1861 gcgcgactgg caatacctgc ggcatgcgtc acctgccggc tgatatcgct gatcgacgcg
     1921 ctcaactcct ccgatgcgga ggcgacattc tgcacattgt tcgaggcttc ccccgaagct
     1981 ctcgcggcct gatgaacctg cgcattggtc tggctggata ccatcgacat gtcatccgag
     2041 gtcgtccgca tctgatcgga tgcggtgtcc aaggcattga gcgcctgccg tatctgggct
     2101 tcgaaagcgg caatatgggc ttcgatggct cgctgtcttg ccgcagcccc gagattgcgg
     2161 tcccgctcct gctgctcgat gcgcgctttc tcaattgcct gctgcttgaa cacgccgagg
     2221 gcgccggcga gggctccgat ttcgtcacgg cgttcggcgt agccggcgtc gactgaaaga
     2281 tcgcccgccg ccaccttcag catggcatcg cgaatggcgt gcagtggatt gatcacgcga
     2341 tgactgaccg tcatcatgct gcccaatgcc agcgcgagag cgccggccag cagagacagc
     2401 tgcaagacaa gctgttcccg cgccgccgac cactgcgcgg aagcccggat ctcggcctgc
     2461 tcgagcgcgc tctcggcgac agtcaccgcg gttgccatgc gcccgaccgt gatcgggctc
     2521 cattgacttg cgttgatctc ggctttttcg ccagccacca gcgcgttcgc gatccggtcg
     2581 cgcagggcca gatattgagg atcgaagctg gcggtcttcg ccgtcgccag cgcgtcgacc
     2641 agaccggacg gcagcttgct gcccgccgca gccaactcca gcgccgccca ggcggcttca
     2701 atgcctccga cgaatttggt gtagctctgc cgcgcctccg gcgtcatgcg cccgaccggg
     2761 agtgcgctcg ccagcagcag cgaggcttcg cccgacatgc tgcgaaacag ccaggcggcc
     2821 tgtttgatcg acagcaactg gtcgatgacg ggatcgttgt ggtttacggc ggcggcgagc
     2881 cgcgcagaca ccgtctccag cgtctgcagc agcacctccg cggtctccat gtattccttg
     2941 aggagggccg gacgacggga ggccttgggc tggctcatcg cctcccagga ctcggcatgc
     3001 aaggctgtca tcgtctgcac caggcgcgac aacgccggga tcagcgtctc cttgtcggcg
     3061 aagtcaatgg cgggaagcag ttctatggtc gcccgcattg ccggcatttc gtcaccccgg
     3121 atggtgcgca gatagctggc catgtcgggc tggatgacct gatcactggt cagcaggcgg
     3181 ctggtgcttg gccggtcgtt gcgcaaatta tgcatcgcct tgaaggcgct cgccgaggct
     3241 tctgcgataa ccgttatccg gccggccgtc tgcagccgct gccaggaggt ccagccggtt
     3301 accgagagaa ccgcgaccac gcaggcggcc atcaggccga tgactgcttt gagaagtgca
     3361 gatgcgctca accgattaag cattcgattc cccaaggaga tggcctggat aaaagccggg
     3421 aatcgtaaat tattaggtaa ccggatactt atttgtattc caggaaatac tctcccagcg
     3481 gttgtctatg aagcccggta cctgacgccg agtagcgcga ccagaatgcc tgccaacagc
     3541 atggggacag ccgccgcgta gaacaaactg gccgcgctga gctcgtaagc cagcaacagc
     3601 ccgatcagca gcgggccgcc gatcccgccg agcctgccaa cccccaacgc ccagcccact
     3661 cccgtcgagc ggatcggcgc cggataaaaa atggccgcca gggcgataac gctcttttgg
     3721 ccgccgctga tacagaatcc cgcacagaat gccgcgacca tcaggacccc tgcaggagcg
     3781 gaaatggccg gccccatcag ggcaacaaac accacaccgg cgaaataaac caccgcaagc
     3841 gagcggtaag cgccaagctt gtccattgcc ggtccgatcg caaaagccgc gacgattcca
     3901 ccgatcgttg tcatcgaggt ggcgagtgcg accgcgttcg gcgaaaagcc atgccctgcc
     3961 aaaatggtcg gcaaccagct ttgcagcgcg tagaattcgg cgaggttcag accgaacacc
     4021 agccagagca ccagcgtccc gagggtcctg cccgactgga acagactgcc gaccgcgctt
     4081 ctttttgctt ccacttcggt caggaattgc tgcgggcctg cgctcggcag cgtccggtcc
     4141 agcctgcgga caatccgcca gatgccgtgg ggttttgccc gggtacggac cagaaaatcg
     4201 agcgattcag gcaggaagac ataaaccagc accgcgagcg cgagcggagt gaccgcgccg
     4261 atccaaagca tcgaacgcca gccataaagc ggaatcatcg atgcagccaa tccgcccgcg
     4321 gcgacgaatc ccagtgagaa gccgcaataa atggcgagta cgaacgtggc cctcagacgc
     4381 ctgggcatgt attcggtggt cagagcgacc gtactcggta tcgcagcccc caaggcgatg
     4441 cccgtgagaa accgcagaac gatcagctcg gtcacgttgg tggcgacgag cgtcaccagc
     4501 gtcagcaacc cgaacgccac cgtgctgaag aggatgagcc gccgatgccc gaacctgtcc
     4561 gacagcggtg acagcaccag atagccgaac atcagacccg tgagcgccga ggaaaagatc
     4621 ggtcccagca cttcccggga aagtccccac tcctttgaga tcatgggcgc catgtagctg
     4681 atggcctggg tatccagccc gtccaggaac atcaccagcc cgcaaagcac aacgatgccg
     4741 tactggactg cgccgatggg gcgggaattg ataaaggcag gaacgtcgaa cgcgtcctcg
     4801 gtcttgctca cggttttttc ctcgaatttt ttgctgttgt ttcgcggggc gagcggttca
     4861 gccagggacc gccggatcgt ttcccgaggc cacgaaggga tctgcgaaga agtcgccgtc
     4921 ggagagacca gccgtcgtcc ggaaatcgcg tcgcgcatgg cggatcatga gtgggttgcc
     4981 gcaggcataa acctcccacc ccgacaaatc ggtcagatcc ctcagcacgg cgtgatggac
     5041 caggcccacg gctccgttcc agtccgggtg gggttccgac aggaccggga taaacttgaa
     5101 ccagcccgcg cgcgcggccc acttccgcgg cagatcggcc aggtacagat cgcgctgacg
     5161 gcggccaccc caatacaggt gcaccggacg tttgttgcca cgcttgatca gatcttcgac
     5221 gatcgacttc aacggcgcga agccggttcc ggtcgcgaga catacaaccg gcttgtccga
     5281 atccgggcgc agatggaaat ccccgtaggg caattcgacg tcgagcgtgt cgccttggcg
     5341 gagcccggca agtaccccct ccgagaaacg tccccccggc acctgccgga tatgcaactg
     5401 cacgccgtcg ctctcgtgcg gcgggttggc catcgaaaaa ttgcgggtgt cgccgtcagg
     5461 catgttgatg cgcagatatt gcccggccag gaattttgcg cgaatgcccg ccggaaaacg
     5521 caagtgcagg atggcgacat cctcgaccgg ccgatggatg cgatagacct tggccgtcac
     5581 cgtcttgcgg gcgtcggggt cgcggcgctg gattctccgg ggatgaataa gaacatttga
     5641 gcggggcctg gccttgcaca gcagaacgtc atcgctggga ccagcgaaat gccttgaacc
     5701 cacggcgact tcgccacggc gaaggtcgcc ctcgcacgtc gcgcagacgc ctttccggca
     5761 tgaatagggc agcgcgtagc cggcgcgctc ggccgcatca agaaccgttt cgccgggctc
     5821 gcaggggaat tcgatatcgc tggcgtcaac ggcaaccgtg aacgtcatgc tgtcgccgcg
     5881 tcgtcgaggc gagacgctcc gcataccagc tggatggatc gcaacaccgc ctcgggctcg
     5941 gcgacgccgc ggcccgcgat gtcgaaggcg ctgccgtggc cgacacttga aaacaaaagc
     6001 ccagccccga tggagagcgc cgccgaattg cgcccggcca gcagttttac cggaatatgt
     6061 ccctgatcgt ggtacatcgc gacaaaggca tcgaacccct gcctgccgag catgagatcg
     6121 gcgccgagcg gtccctcgac atcgagaccg gattcccgca aacgcctcgc cgccggaacg
     6181 gtgatccggt catcatcgtc gccgaacagc cccccttccc cggcgtgggg attgataccg
     6241 aacagtccga tgcgcggccg cttgatctgc agcctctgca gagcggcctc ggcggttcgc
     6301 gccgcgcgct ccaccaactc gggcgtgatc cgggccagcg ccgcgtgtaa ccgctcatgc
     6361 agcgtgacat gaacgatccg cagaccgccg ccgaccagca tcaggaagac ctcatcctcc
     6421 gggaccttca tcagccgcgc gagaaggccc ggataaccgg agaacgggat gccggctgca
     6481 ttgacgttgg tctcggaatg cggacaggcc acgatcgcgg cagcgtgacc ctccgacaca
     6541 aatcgcatcg ccgccgcgac atagtcgacg gtcgcccgcc cgcctgcggc caacggcgtt
     6601 cccgggcgga agtcctcccg ggacaaggca tcgacggcca ggaactgcaa ggcgccagcg
     6661 cgcgcagtcg atccgtcgaa cttccgaatg gaaaaatcct tcgcgcagga ctccgcgtag
     6721 gaccggacga caaactcgtc gccaatcagc acgaccgccg cgccgccctc gcgcgcaagt
     6781 ttggcggccg cctttatcgc gatctccggc ccgatgccgt tcggatcgcc aatggtcaac
     6841 gcaatggcgg gaggcgtcac aacggcagcg ccagcagcgt atcgatgcgc gaactgtcgc
     6901 acaccaccgt ccgctcgttc agcttcacct gaccgtcctc gatcttgtag aggtcgagat
     6961 atcgcccggt tgcaaagatg tccgtatcgc cgtcctgcaa gattcgcgtg accaggaacg
     7021 gcgtttcgct gcgcacttcg tcgttctgtt cgaaaagtat cgagggctgc cccaggatat
     7081 gacggtacgc ctgacgctcg taaatgtttg cctcgcgcag cgccgaaacc cgatcggcaa
     7141 gcatgccccg cgaattcgcg aagataagtc cagcctccag gccttcgcgg tggttggccg
     7201 ctgtcgtgac cctgtagata cattgtccgt ggaaatgatt gggccactct tccagccggt
     7261 cgtcgtcgat gcatcgcgcg tattcggcct gggcgcgggc gatcatttga tacacattgt
     7321 cgatcatggc ttccgtcccg ctttcacacg cccatattgc tgcgataggc cttccagaat
     7381 ccgcgaacgg aagcttccgt aacccggctc tcgctgctgc gggcctcacc gccgcccatt
     7441 tcgagaaccg cctcctgatc cgatgcgccg gcgatgccgc gctgaacgaa accaccgacg
     7501 cagccatcct ccatggagat gtagcccgcg ggcccaacga gattgccctg cttgacgcgg
     7561 gtcaatcgct gttcggcggt gtcatcggcg aatccgatat aggtccagtt cagatcggtc
     7621 ttgtcgatgg cgcgcggcac gatctgccgg accgcgatcg cgttttgaat ttgctgcaga
     7681 acgaaggtgg gaaagaccga taatatctgc agcgtgacgc cgtcgtgata ttcgtcgaac
     7741 ccttgcagaa gcgaggggtc ggcgagcttg tattggcttt ccgagcgcag ctgctgcttg
     7801 gcgtattccg catccttccg ggcttcgcga tcgatggcgg aataactgac gtgatggccg
     7861 ccgctctcat cgacgataat ggcgcccttc tgcgacagct tgttcagttc gaaggtcgtg
     7921 aagaacaggt gcagaatgct ggcatggtag gagtccttga cgttctccat gtagagcttc
     7981 cagttgttgg gcagcacctg cgtgatgcgg ccgatcacaa ccggggttcg acctcccagc
     8041 acccgttcga tgcgggatga aatttcctcc ccgagatatt cctcgatcgg agcaacgtct
     8101 tcggagaacg atccaaagat aagaccgttc aacgtggcga tccgcagttt gcgcgggcca
     8161 tggtcctcca tccggaaact gtcgggcata ccgccctttc ccttgacgcc gtccttgaac
     8221 gccacgccct tcaggttgcc ctgccggtcg taggtccagg cgtgatagac gcagctgaag
     8281 tccttcttgc ccttgccgtg gttgtccaga cagatcaatg cgccgcgatg cgcgcagcgg
     8341 ttctcgaaag cgtagatctc gccatcggcg tcgcgggtga cgatcaccgg cgtgtcacca
     8401 accttcgtgg tgcggaaatc gccgggctcc ggaacctcca cttcaagcgc gaggtagtgc
     8461 cagctcggtc cctggaagat gagcttctgc tccgacttgt aggcctcttc ggtctggaac
     8521 acccggtagg gcacgcgcgt cagcccttcc tgcggccagg cctgcccacc gttcgggatc
     8581 tcttgatgct gcgccatgat caaacgcctc tgctggccgg atcggttgac tttcgaaacc
     8641 cgaaggtgtt cggatatcga acatatagct ttaatccgaa caggtttgac cgtttccaaa
     8701 ccgctgtcaa gggtagcctg atgccgcaaa acaccgaacg gcccgcacgc aaggccgaag
     8761 gaatgggcgg cctcgccaag gggcttgcga ttgtcgaggc gttttctgct catcccgtca
     8821 tgagcgtcgc cgacgcggcg cgagcggcgg gcgcgacgcg cgcatccgct cggcggtgtc
     8881 tcatcacgct ggccgaactc ggctatgtcg aacattccgg gcgggaattt cgccctttga
     8941 cccgtcttcg caggctggga ggggttgcct cgagacgcga tcagttcgcg caattggcgc
     9001 agccattgct cgctcgcgcg cgtgacgcgt tggctgagtc ggtttcgctc gcggtactcg
     9061 atgatgatgg ggcactgttc atcgcgcgcg cggaagcaga gcatatcgtc tcgaccggcg
     9121 tccgcgtcgg cgcccgcctt cccgcctatt gttcggctac cggccgcgtg ctcctcagcc
     9181 aatttccgga ccaggagatt ctcagccgca tcggccgaaa gccactggcg cgacggacgt
     9241 cgcacaccct gaccaaacca tccgaggtgt tggccgaggt tcgatcggtt cgcgaaaaag
     9301 gttacgccgt cagcgacgag gagcttgaac tcggcctccg cgcgctggcc gtaccggtga
     9361 cgggtgcaaa ccacgagatc atcggcgccg tcagtgtcag cgcggcatcc gctcgcgttc
     9421 ggagcgcgga attgcgaaga cgcttcgtgc cggtactgcg atcattcgcg gccatgctgg
     9481 ctgaagcttt tcagagcgtc gattgaggct tggctggaga cggacgcggt gtcatggcag
     9541 gacttgatcc ggccatccat cattcttcga aagagttctt ccaaggagtc ttggaagcta
     9601 tggacccccg ggtcaagcca agcccggggg tgacgcgctt tagcgatact caaaagagct
     9661 ttggcgatat ccccaaagag aaaagtcccg cctcgcggcg ggaccttact tctctcgctc
     9721 atgtcagttc cggctagcgc tcggtctgct gacccggatt gggtttctgg cccggacggt
     9781 tgggatcctg ctgctgctgg ccgggcttct ggccgccgcc ctgctgctgg cccggcttct
     9841 ggcccggctt ctgacccggg ttctgattct gctggcccgg atttgggttc tggttcgaca
     9901 tcgggatctc ccttgctgaa cgtgacgtcc gtgttgaacg caacgggagc gatcaacccg
     9961 ggagaaagca attggtttca ggaaacgccg gttccgtcgc ggtggtttga acggcgatgg
    10021 cctgtcccaa ctgtggccga aggaacccta gactgaacta cctatgcagc ggcgcaattc
    10081 gtgctgttgc cgcaccgggt tcccactgtt agaaaacgaa acgacgcggc gttccggctg
    10141 ccggggccat cgccgcgtca tgcaaacaat agcggcagca catggactat ttcgcccagc
    10201 aactcatcaa cggtattgtg ctcggctcga tctatggcct gatcgccatt ggctacacca
    10261 tggtctacgg catcgtcggc atgatcaatt tcgctcacgg cgatatcttc atgatcggcg
    10321 gcttcatcgc gctgatcagt ttcctgatct tggtctcgat cggcctcacc gtggttcccg
    10381 tgatcctgct gatcgtgctt ctggtatcga tggcgatcac cgcgctttat ggctggaccg
    10441 tggaacgcat cgcctaccgg ccgctgcggc actcgttccg gcttgccccg atgctttcgg
    10501 cgatcggcat gtcattcgtg ctgaccaatt attcgcaaat cgcccagggc gcgcgggtga
    10561 aaccggtgcc gccgatcatc accggcggct acaccctgtt cgacagggac ggcttcatcg
    10621 tgcggctgtc caatgtgcag atcatcgtcg tggtcaccac catcgtgctg ctggcgatct
    10681 tcacctggct ggtggcgcgg acccggctcg gccgcgacat gcgcgcctgc gagcaggacc
    10741 agaccatggc ggcgctgctc ggggtcgatg tcgaccgcac catctccatg acgtttgtca
    10801 ttggcgcagc actcgccgcg gtcgcgggca tgatgtatct gctctattac ggcctggtgg
    10861 atttcttcat gggctttgtc gccggcatca aggcgtttac cgccgcggtg ctcggcggca
    10921 tcggctcgct gcccggcgcg atgctgggcg ggctccttat cgggctgatc gagacgctgt
    10981 ggtcggcgta tttctcggtc gaatacaagg acgtcgcggc gttctcgatc ctgatcattg
    11041 tgctgatttt ccttcccacc ggcctgttgg gccgacccga agttgaaaaa gtttgacgga
    11101 caccggcgtg acagcacccg tgcccgaacc gacgctcgct ccgcgcgccc cggacgtcgc
    11161 cttcatcttc aagaaagcct tgatcagcgc gctggtcgcg ctggtgctgt tctcgctgat
    11221 gatcggtatc cgcaccgaag ccggacccgc cggacagttg atctactgga cgaggttcgg
    11281 cgatcttgcc gccgttgtcg cggcggtatt cggcggcagc atcatcgtcg aaatgctgcg
    11341 gctgtggtgg ggaccggtcg gcaccatccg catcgttccg ccgtcggtgc aaagcacgct
    11401 tgcggtcgcg cggcgcgcga tcgcgccggt actgctgatc ttcacccttc tggtaccggt
    11461 gatcttctac gatcagcgct acatcctcga tctcggcatt ctggtgctga cctatgtgat
    11521 gctgggatgg ggcctgaacg tggtggtcgg cctcgccggg ctgctcgatc tc
//
LOCUS       DATNOI010000323        30451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_210112,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000323 DATNOI010000000
VERSION     DATNOI010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 30451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30451
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210112"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1722
                     /gene="istA"
                     /locus_tag="VN968_12700"
     CDS             202..1722
                     /gene="istA"
                     /locus_tag="VN968_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_058167704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HXL30133.1"
                     /translation="MRKVREVLRLKYACGASERVIARSVGIGRTAIGEYIRRAAVIGI
                     TWPIPEELDDTALERKLFAPTGYNPSRSKPLPEWGHIHAELRRRGVTLALLWEEYRGH
                     HLDGYGYSRFCDLYVEWRHGITATMRQTHAAGEKLFVDFAGDTVLVFDGLTGEVRAAK
                     IFVAVLGASNYTFAQARFSEALPDWIGAHVDALAFLGGVPKALVCDNLKAGVTAASRY
                     EPGVNRTYQDLATHYGSTIMPARPRKPRDKAKVEAAVLIVQRWILARLRNRRFFSLAE
                     LNLAIRILVDELNARLMRKLGASRREFFDTIDRPALMPLPAEPYQYAEWRRARVAPDY
                     HVEVQGHFYSVPSRMIRQVVEVRATEATIEVFHRGNRIASHARSGVKHRHTTIPEHMP
                     SAHRRYAFWTPARLLAAAEKIGPSTVALCEAIMRTKPHPEQGFRSCLGILRMEKTYGA
                     QRLEAACRRGISIGAASYRSIASILKTGLDKAFLPETNPDADPIQHGNIRGRGYYH"
     gene            1745..2480
                     /gene="istB"
                     /locus_tag="VN968_12705"
                     /pseudo
     CDS             1745..2480
                     /gene="istB"
                     /locus_tag="VN968_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
     gene            complement(2772..3866)
                     /locus_tag="VN968_12710"
     CDS             complement(2772..3866)
                     /locus_tag="VN968_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014328597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXL30134.1"
                     /translation="MGTIIARKRKDGSTGYHAQVTIKRSGEIVHRETRTFDRRQAASA
                     WMEKRETELAKPGGLERQNMPDPKLSAVIDRYIDESIKEIGRTKAQVLATIKDYDIAD
                     LKCSEVTSEHLVAFAKKLPVQPQTVGNYFSHLGSIFTIARPAWGYPLDSQAMDDALVV
                     TRNLGLTSKSKERDRRPTLTELDLLMHHFGKVKTHRPRSAPMQQIIVFAIFSTRRQEE
                     ITRITWTDFEAASPPTHPDARVLIRDMKHPGDRQGNDVFCDLPPEATAVIESMPRVAK
                     EIFPYSVDAIGAGFTRACKFLGIDDLHFHDLRHDGISRLFEMGKTIPQAASVSGHRSW
                     SSLKRYSHLRQTGDKFASWKWLQLVTRGES"
     gene            3994..5013
                     /gene="dusA"
                     /locus_tag="VN968_12715"
     CDS             3994..5013
                     /gene="dusA"
                     /locus_tag="VN968_12715"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HXL30135.1"
                     /translation="MTDKTHKYSKISNFAFSVAPMMDWTDRHCRVFHRLMTRRARLYT
                     EMLTTGAIIHGDRRRLLGFDACEHPVALQLGGSDPRDLATAAKIGEAFGYEEVNLNVG
                     CPSDRVQDGRFGACLMAEPALVAECVHAMKRAVDIPVTVKCRIGIDDQDPEIALDALA
                     RGVIAAGADALIVHARKAWLSGLSPKQNRDVPPLDYDIVYRLKAALPDMPIIINGGIG
                     NLGEAKAHLRFVDGVMLGRAAYQEPWRLLGADPELFGEAAPHPTMKHVFEAMVPYIES
                     ELAQGTRLHSITRHFVGAFHGVPGARAFRRHLAENGVRSGAGVNVLRDAIALVEDSVV
                     APIAA"
     gene            complement(5163..5888)
                     /locus_tag="VN968_12720"
     CDS             complement(5163..5888)
                     /locus_tag="VN968_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30136.1"
                     /translation="MEMIKCISVIAASALGLISLVLPVAAEQPTKIDFSGETVGAEPK
                     SLVAVVGVWRIESEGGKNVLAVDGRQWKEGQSSAGIADKARALYGERYAEFLDRVQAY
                     AYYPYTVTNDVENFRGGDITVRFEGLSGRIDQGAGILFNLKPNGDYLALRANCLENNL
                     VLWKFEKGKRSSVAWIRNAPAASKQWHDLKVRVSGTKVEGYLDGKLYLEHTLPEAVSG
                     RVGLWSKADSYMHFSDYTVTPPN"
     gene            complement(5879..7261)
                     /locus_tag="VN968_12725"
     CDS             complement(5879..7261)
                     /locus_tag="VN968_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL30137.1"
                     /translation="MHSPETFHVQNAPLRPLLVKELWEILSGRALWTMLLLMCPLLGY
                     SFLQATSLYGEASVAGLQSPVLASSLSPLDGVLVPTLGASYVAITLLFPFVAIRVLGQ
                     EKESGALRLLVQLPYRPPALIAAKLAAVLAALVLSSIPAFSALVIWGMQGGHLSAPET
                     LNLLFGHLLYGLLVGAIALFAASVSESAATAAIVTLAFTIGSWVLDFTVAGQPGFLAW
                     IARLSLTQVLRTFEQGLLSAGLVLGIGAAMFGFAALANVWLPPGVQVRDKVIGSIAWV
                     VVVALVLGLATQIKLTFDVTEDRRNSFPAADQHLLASLAQPLVVTVNLVPEDPRYADL
                     RRNVLAKLERAMPDVSIRLASGRQGQAATAGEDRYGEVEYAYGARSDVSRSTSPREIL
                     PMLYGLAGVSPPAPAPGSDYPGYPLVAGRRVALLWFFAGLPLCIALAWRWNRRLPNHR
                     TIMKEIHPWR"
     gene            complement(7248..8006)
                     /locus_tag="VN968_12730"
     CDS             complement(7248..8006)
                     /locus_tag="VN968_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30138.1"
                     /translation="MDGVLPIVAASAFGQARDPVLLRVDSITKQYADQAVLSDVAFDV
                     PAGEILGIIGPNGAGKTTLLEALAGVLPAETNDVRWRGERLPASRRREAMFYLPDGVR
                     PYLDQPVTRVLSFIAGVYRRSTGEIADAIKSVGLTPVLHKRVHSLSKGYGRRLMLALG
                     LLTPHPLLLMDEPFDGFDLRQIRTIVDVLRREASEGRTLALAIHQLPDAERVCDRFIL
                     LADGRVRGVGTLNDLRARTGISEGSLEDIFLALT"
     gene            complement(8182..8694)
                     /locus_tag="VN968_12735"
     CDS             complement(8182..8694)
                     /locus_tag="VN968_12735"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HXL30139.1"
                     /translation="MSRVLLVTLILAATVGAVVAYADPEQIARASDTVSEVMRKRAAS
                     PARAVQIPRGQTGEFALRAKINGANAPMVIDTGATSVVLTWETAKAAGLPLELLEYDV
                     DVETAGGHTKAARLTLDRLAIGKLVERSIPALVVPRGQMKTNLLGMSFLDRLESWEVR
                     ADRLMLHGYP"
     gene            complement(8738..8947)
                     /locus_tag="VN968_12740"
     CDS             complement(8738..8947)
                     /locus_tag="VN968_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HXL30140.1"
                     /translation="MSKETPCIAVCMIDPKTSLCFGCGRTLPEIARWHRMASAERHAV
                     MAQLAARMTNAGLAQPGMTVGPKPE"
     gene            complement(8993..10585)
                     /locus_tag="VN968_12745"
     CDS             complement(8993..10585)
                     /locus_tag="VN968_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXL30141.1"
                     /translation="MSNPADKPEVFQLPAETPIAPPVNNRRAAAQRVREARDRLTSTS
                     GTRPAFDRELLRQHAQTRISASYVVMLLVVATGLLFGFWMHPVSAAAWTCGMLCIHAA
                     IIRNCLRFLGEPASLTATRKWRTRFVLLDLLYGLCWTAILTHPASIDAVSNTLMMFLM
                     LLVIAVSSMLAASLPIAALAATVPVTSAIALNFALSGTFDHYVLAVLALIAEGYFALL
                     ARRLHSTTLATLEARAEKDALIGELEQAKSISDEARHRAESANVAKSRFLAQMSHELR
                     TPLNAILGFSEVMKSEIFGPHAVPVYKEYSADIHNSGVHLLNLINEILDLSRIEAGRY
                     ELNEEAVSLVHVVADCHHLLKLRASSRGITIHEVFEQGMPRIWGDERAMRQIVLNLLS
                     NSIKFTPQGGEIWLKVGWTASGGQYLSVKDTGSGIAEDEIPIVLASFGQGSNSIKSAE
                     QGAGLGLPIAKSLVDMHGGTFTLKSKLRIGTEVIVTFPPERVMSALAPMAEEAPPLQP
                     EATIDERRRARNKPIMSAGTGL"
     gene            complement(11003..11872)
                     /locus_tag="VN968_12750"
     CDS             complement(11003..11872)
                     /locus_tag="VN968_12750"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL30142.1"
                     /translation="MAKVAFLGLGVMGFPMAGHLVAKGGHEVTVFNRTPAKALAWAEK
                     FAGKTAATPKAAAEGQDFVMACVGNDNDLRAITLGADGAFAGMKKAATFVDHTTASAE
                     IARELDAEATKRGFKFVDAPVSGGQAGAENGVLTVMCGGSEDAYAGAEPVIAAYARMC
                     KRLGPAGSGQLTKMVNQICIAGLVEGLSEGIHFAKKAGLDITAVIDTIAKGAAQSWQM
                     ENRYKTMNEGKFDFGFAVEWMRKDLSICLAESRRNGASLPVTALVDAFYSEVEKMGGK
                     RWDTSSLLARLER"
     gene            12094..12534
                     /locus_tag="VN968_12755"
     CDS             12094..12534
                     /locus_tag="VN968_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL30143.1"
                     /translation="MSVSVGVLDHFNIRTRKLADTVRFYEDVLGLEKGARPNFAFPGA
                     WMYSEGKPVVHLVDISRTDEPQKPDSGVVHHVAFASQGFAGMRQRLETKGMEFDSRQV
                     PGGDLWQIFVNDPNGVMIELNYEAAKEQSAATAPTERADDVGVR"
     gene            12591..13721
                     /locus_tag="VN968_12760"
     CDS             12591..13721
                     /locus_tag="VN968_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HXL30144.1"
                     /translation="MSVTQQQVQDALAKVASPRGVALTNANVLSAITVTDGKVFFSIN
                     VDAAEARAWESVRARAEAAVRAIPGVNVAMIALTAERKPGAAAAPPPHRHAQGVPHVS
                     SHKPPQSPVSPMSKQSEIPGIAAVIAVASGKGGVGKSTTALNLALGLRDLGLRVGLLD
                     ADIYGPSVPRLTGIRQKPQLNDDKKMIPIQRFGLAIMSIGFLVEEETAMIWRGPMVMS
                     AITQMLRDVAWGTLDILVVDMPPGTGDAQLTLAQNVPLKGAVIVSTPQDLSLIDARRG
                     LAMFKKVNVPVLGIVENMSYFQCPHCGTRSDIFGHGGARHEAERLAVPFLGEIPLHMS
                     IRVTSDSGTPVVESEPDGAHAAIYRAIGAKVRDQLQGVIAAA"
     gene            13908..14996
                     /locus_tag="VN968_12765"
     CDS             13908..14996
                     /locus_tag="VN968_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXL30145.1"
                     /translation="MKRRDFLKVSATGAAVAAVASPAIAQSSPEIKWRMTSSFPKSLD
                     TIYGGAEYFAKQVAEMTDNKFQIQVFQAGELVPGLQALDATAKNTVEMCHTVSYYYVG
                     KDPTFAIYASVPFGLNARQQNSWWYQGGGMEIGNEFFKKAGVIGFPCGNTGTQMGGWF
                     RKEIKTVADLSGLKFRIGGIAGQVLQKVGVVPQQLAGGDIYPSLEKGTIDAAEWVGPY
                     DDEKLGFQKVAKYYYYPGFWEGGPTVHAFANLDKWNELPKSYQAIVSNACANANSWMA
                     ARYDMQNPSALKRLVAGGTQLRPFTNEVLEACLKATNELWAEISAKNPDFKKSIDAMQ
                     AYRSDQYLWWQVAEYTFDSFMIRSRTRG"
     gene            15171..16259
                     /locus_tag="VN968_12770"
     CDS             15171..16259
                     /locus_tag="VN968_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXL30146.1"
                     /translation="MKRRDFIKVTGIGVAGAATMAAPAIAQSTPEIKWRMPTSWPKSL
                     DTLYGGAETMAKVVAEATDNKFQIQTFAGGEIVPGLQVLDAVQNGTCEIGHTASYYYF
                     GKDPTFTFGSSVPFGPNMRINQAWYMLGGGKEVLNEFYKKYNVTSLLAGNTGCQMGGW
                     FRKELTSVDDLKGLKFRIGGFAGRVLQKMGAVPQQIAGGDIYPALEKGTIDGAEWVGP
                     YDDEKLGFYKVAPHYYYPGWWEGGPMLLSFVNLDKWNALPKYYQSVLEQAGHYANNWM
                     MAKYDQSNPQALRRLLANGTKLHAFSPPIMEASLKAAKELHGEVAATNADFKKVYESL
                     TAFSSNSYQWFQVAEVGYDNFMARHSQS"
     gene            complement(16343..18001)
                     /locus_tag="VN968_12775"
     CDS             complement(16343..18001)
                     /locus_tag="VN968_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXL30147.1"
                     /translation="MAAFLIANMAPLMFASLVVVLLLGYPAAFSLGAVGLIYAFVGIQ
                     LGEFRPDFLQALPERVYGVMNNDTLLAIPFFTFMGLVLERSGMAEDLLDTIGQLFGTI
                     KGGLAYAVVFVGALLAATTGVVAASVISMGLISLPIMLRYGYDRRMASGIIAASGTLA
                     QIIPPSLVLIVMADQLGKSVGDMYEGAFVPGLVLAGLYAVYAFIVTLVFPKAAPGLPP
                     EAIGFREKDGSRGLISLLMLFIASCFFGWFMMRSSETHGADFVVLSMFFGILFAFFVA
                     VLNWIIDKFTGFRFLSAMAQQTTFVMVPPLFLIFLVLGTIFIGLATPTEGGAMGAAGA
                     IILGAAKRRLSWDLIRQATESTAKLSAFVVFILVGARVFSLTFYGVNGHVWVEHLLTS
                     LPGGQIGFLIFVNAFVFVLAFFLDFFELAFIVIPLLGPAAEHLGIDLIWFGVILGVNM
                     QTSFMHPPFGFALFYLRSVAPKDSYLDRVTGKRMDPVTTGQIYWGAVPFVVIQVAMVL
                     LVIMFPSMVMHYKGALSTVDPNTIKIDIPQIELPPLDFGPPPKQ"
     gene            complement(18031..18612)
                     /locus_tag="VN968_12780"
     CDS             complement(18031..18612)
                     /locus_tag="VN968_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXL30148.1"
                     /translation="MQSLLKLSRGIDAFTRWMGKRLAWLILVAVVVSALNAIVRKTFD
                     TSSNSWLELQWVLFSIVFLLCSSWTLLDNEHIRIDIVNNMMPKQVRNSIDIIGHAFFL
                     LPLTIIMIITGGPFFMRSVEINEQSGNAGGLPQWPAKSLIIVGFTLLLVQGISELIKR
                     IAVMRGLIPDPHASQVNALEAEVEHLVEAIKKD"
     gene            complement(18803..19837)
                     /gene="moaA"
                     /locus_tag="VN968_12785"
     CDS             complement(18803..19837)
                     /gene="moaA"
                     /locus_tag="VN968_12785"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HXL30149.1"
                     /translation="MNGPPVSPPTSLSRAMTDPFGRTISYLRVSVTDRCDLRCVYCMS
                     EDMTFLPKADLLTLEELDRLCTAFIAKGVRKLRLTGGEPLVRRNVMSLVRSLSRHLDT
                     GALKELTLTTNGSQLARFASELRDCGVKRINVSLDTLDPAKFRAITRWGDLDKVLTGI
                     EAARSAGLAVKINAVALKNLNEEEIPSLMQWAHGKGMALTLIEVMPMGDIGEGRIDQY
                     VPLSLLRARLAKQYTLTDLDEDSGGPARYVRVSETGGKLGFITPMTHNFCESCNRVRI
                     TCTGTLHTCLGHEDASDLRKPLRASVDNDLLSSAIDSAIGLKPKGHDFIIDRRHNRPS
                     VSRHMSVTGG"
     gene            complement(19975..20442)
                     /locus_tag="VN968_12790"
     CDS             complement(19975..20442)
                     /locus_tag="VN968_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30150.1"
                     /translation="MRRVIAIAVTGYSLAGCSSFASLDAFKPTPPTVQVQLDSVPSGA
                     DARTSLGPGCKTPCAVAVSAPDSGFSVAYTLNKFQPATVPVQVIRIPGDFASPGSTTV
                     DPNPVVAELQPAAPPPRAAGKKILTPKKPKPPKAAAAPAGAPEPPPTPAPTTR"
     gene            complement(20598..21284)
                     /locus_tag="VN968_12795"
     CDS             complement(20598..21284)
                     /locus_tag="VN968_12795"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXL30151.1"
                     /translation="MTSPRTIVFDLDGTLVDTAPDLINALNYVLDREGLPPVPLHAAR
                     NMIGAGARRLIERGLELEGRSVGPEDITRLTSDFIDYYAAHIADASRPFDGLESALDD
                     LRERGYRFAVCTNKLEWLSKLLLDRLGLSSRFSAICGADTFGVSKPDPAILQQTIARA
                     GGQLSAAIMVGDAGPDIGVARRAGVPVIGVEFGYTDVPIADLKPDLHINHMRDLPAAV
                     ESLMFPRNSS"
     gene            21521..22219
                     /gene="rpiA"
                     /locus_tag="VN968_12800"
     CDS             21521..22219
                     /gene="rpiA"
                     /locus_tag="VN968_12800"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HXL30152.1"
                     /translation="MSMDGLKRQAAGRALEFVADGMKLGLGTGSTAKHFVELLGERVR
                     AGLDVIGVPTSEATRADAVRCGIPLTTLDDIDRLDLTVDGADEIDSGLNLIKGGGGAL
                     LREKIVAAASDRMIVIADDSKWVEVLGRFPLPIEVIPFGLAATRRAIGGAFAESGVSG
                     QMVVRKTGAGHVFVTDGGHWIVDAHLERISDAPHLARLLNSIPGVVEHGLFIDLASTA
                     MLAGSQGIRVVERR"
     gene            22245..22772
                     /locus_tag="VN968_12805"
     CDS             22245..22772
                     /locus_tag="VN968_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HXL30153.1"
                     /translation="MKRLFAILSAAGLVLGLALAGLPAGAQQPAAPALKPGTPAAIAA
                     AKEILAMKNAGAMYANAIPNIVQQTKDVLLQNNLNYQRDLNEVAVIVAQGLAGREKEI
                     GEGMAKIYANEFTEQELVALVTFYKSPLGQKLLASEPRAIQFSMSYMNQWAQGFAEVV
                     NGQFRAEMRKRGKEI"
     gene            22816..24204
                     /gene="gor"
                     /locus_tag="VN968_12810"
     CDS             22816..24204
                     /gene="gor"
                     /locus_tag="VN968_12810"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HXL30154.1"
                     /translation="MAEFDVDLFVIGGGSGGVRAARIAAGYGARVMVAEEYRMGGTCV
                     IRGCVPKKLFVIGSHIRHEIEDAAGFGWLIPPVTFDWPTLVANKDKEIARLEGIYTSN
                     VEKSGARIVKTRAVFEDAHTLRLATGETVKAKYVLIATGGAPNHGPAIPGIEHVISSN
                     EVFHLKELPRRIVIQGGGYIALEFACIFAGFGTDVTVIYRGDNILRGFDEDVRAHVRA
                     EMERQGITILTGCTVNKVDKPGREFISHLSNGSSIASDLVMFAIGRHPNVAGLGLEHA
                     GVAINPNNGGIAVDGWSQTSAPNIYAIGDVTHRINLTPVAIREGHAFADTVFGKRPVQ
                     VDHTNIPTAVFSQPEVGTVGLTESQARAQFSHVDIYKTDFRPIKSTMSGRDTRILMKL
                     VVDGTTDRVVGCHIVGDTAAEIVQAVGIAVKMKATKADFDATFALHPTAAEELVTMRT
                     PTARYVREAAAE"
     gene            24225..24788
                     /locus_tag="VN968_12815"
     CDS             24225..24788
                     /locus_tag="VN968_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXL30155.1"
                     /translation="MTAGFVQTYRAFAYNNAWANHRLLTACANLNQQAFEAERTGFFP
                     SLQKTLNHIYIVDLFIVDALEGGWLGPKAFANRVPCPSLAELRRAQAAVDKRLIAVCD
                     TLTPDLLDGIVRVNRDPQVQTERRDRLLMHLFQHQIHHRGQAHAMLSATAIKPPQLDE
                     FFSIAGASRRAIEFDGLGWTEETVWGP"
     gene            complement(24898..25119)
                     /locus_tag="VN968_12820"
     CDS             complement(24898..25119)
                     /locus_tag="VN968_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30156.1"
                     /translation="MVDDKVRIKCSKCSQMFRVRAQKVRPGFQVNCQHCNRLITFEFG
                     SEDPNIRRALSSARQITSTLEAAPKAPAN"
     gene            complement(25211..25639)
                     /locus_tag="VN968_12825"
     CDS             complement(25211..25639)
                     /locus_tag="VN968_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30157.1"
                     /translation="MSTRSRQSIFGSRLRGAKIRADGARQEAKKAIREADRAEAEAWS
                     IRMEGYGGPAQPSPTIAQCLNGGYGWLQVQCRRCETEASIPLEHVRRPRETPIWKLEA
                     ALKCRSCRTPRYSPPVHMIRLTEQRQTAPYLWVHPDDNDR"
     gene            25731..26408
                     /locus_tag="VN968_12830"
     CDS             25731..26408
                     /locus_tag="VN968_12830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HXL30158.1"
                     /translation="MCNLYSITTNQAAIAALFRVINQYVGNLPPMPGVFPDYPAPVVR
                     NAGQNTDGERELTMMRWGMPPPPRSGGPPVTNIRNTSSPHWRGWLKPEHRCLVPANSF
                     AEYAPEPNPETKKKDVVWFARDDDRPLFAFAGIWTEFRGDRGTKSKPLPGPHLVYGFL
                     TTAPNRIVEPIHDKAMPVILTTPEEYDAWLRAPWDEAKALQRPLPDEALKIVARGAEK
                     EDVAVSG"
     gene            complement(26439..26681)
                     /locus_tag="VN968_12835"
     CDS             complement(26439..26681)
                     /locus_tag="VN968_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30159.1"
                     /translation="MSDESPPFVHEGLDPAPEPKSTIAEVSEAVRGTANRIGDAIDAG
                     RKPGMPLSILSNIAREAPLASLLVAFLLGVAVARRR"
     gene            27146..27457
                     /locus_tag="VN968_12840"
     CDS             27146..27457
                     /locus_tag="VN968_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HXL30160.1"
                     /translation="MTRKLPKRGLPPMHPGELLREEILPALDRSKTEIAKLLGVSRQT
                     LYDILAEKQPVTPAMALRLGKLCGNGPDLWLNLQKRYDLHQAQQELGETIKKIPTLEV
                     A"
     gene            complement(27677..28120)
                     /locus_tag="VN968_12845"
     CDS             complement(27677..28120)
                     /locus_tag="VN968_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30161.1"
                     /translation="MDYSGFTDDSLVKMYEGVREALAADEAFGEIGGEGRFRVRKTPA
                     WKLHAADLEAEMLRRGITFEVIEWYEADHPVEEMHAPSAVPEHEEMHRPPAVPEQSPP
                     QVEPSKSQKVLASLSTIDVVNVPPPAPTKEKPPRKRRAKASDQGI"
     gene            28243..28392
                     /locus_tag="VN968_12850"
     CDS             28243..28392
                     /locus_tag="VN968_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30162.1"
                     /translation="MPVILTAKEEYDAWMHAPWDEAKALQRPLPDEALKIVARGAEKE
                     DRAAG"
     gene            complement(28484..29404)
                     /locus_tag="VN968_12855"
     CDS             complement(28484..29404)
                     /locus_tag="VN968_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXL30163.1"
                     /translation="MVRKFLIIGSFAAIYLLWGSTYFAIALGLKSFPPFLLMAVRSLC
                     GGLILVALCGREIIQHSRRTWLNAGLSGLMFFVGCHGVLAFVQQTVPSGVAAIVLAAI
                     PLWIVLLDFLLPQERRPAPLTMMALVPGFYGVAFVAWQNIGENGVSILPILWLLGAAL
                     SWSAGTVWSRRTASDGSSALISGMQLVIGGAVLLAISSVTGEVKQFSSQAVTATSLGA
                     VTYLVVAGSVIGFAAYHWLLDNVATPLVSTYTFVNPVVAILLGTIFLKETFSTAMIFG
                     AGLILASIIAMWRSEHATASSPRKELARAG"
     gene            29486..30106
                     /locus_tag="VN968_12860"
     CDS             29486..30106
                     /locus_tag="VN968_12860"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HXL30164.1"
                     /translation="MASISNSPGKLAREELCIKFANTVAWRLRDPVEERAASPQAMLD
                     WLAGAGLADGQMVREVTTRWKQSPRDARVFLKEAVELREAIYSLFVAKIHARRADPVA
                     AAILNAHLAGTVRGLGLTSSRTTLKWCSTSRNVAPEALLKPIAWSAAGLLMGPRAQKV
                     RQCQDERGCGWLFIDESRAQNRRWCSMGDCGNLAKSRRHHRRVSAK"
     gene            30236..>30451
                     /locus_tag="VN968_12865"
     CDS             30236..>30451
                     /locus_tag="VN968_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30165.1"
                     /translation="MVADEVEAGIASIHSQTKSSDVGPIKNVPESHITYKNQVVVSGC
                     DSQELLLVDPSGWLSCRGRGSATICSND"
BASE COUNT         6092 a         9217 c         9496 g         5646 t
ORIGIN      
        1 tgcggaatcc gagccaatgc ggccaccgat tccgacggaa agcggccacc caatccgatc
       61 gaaagcggcc accgattccg aacgaagccg gccaccctgt tgatggggtg aagcagggaa
      121 cgttgtcggc agtctgagcc gggcaaggtc ctccttttgc gattcgcaaa gggaggtctg
      181 atgcccgccg agagattgcc gatgcggaag gttcgagaag ttcttcgcct gaagtacgcc
      241 tgtggggcga gcgagcgggt gatcgcccga tcggtcggaa tcggccgcac ggcgatcggg
      301 gaatatattc gccgcgccgc tgtgatcggc atcacctggc cgatacctga ggaactcgac
      361 gacacggcgc tggagcgcaa gctgttcgcg ccgaccggct acaacccgtc gcgatcgaag
      421 ccgctgccgg aatggggaca tatccatgcc gagctgcgcc gccgcggcgt gacgctggcg
      481 ctgctgtggg aggaatatcg cggccatcat ctcgacggct acggctacag ccggttctgc
      541 gatctctacg tcgaatggcg ccacgggatc acggcgacca tgcggcagac ccacgcggct
      601 ggcgagaagc tgtttgtgga cttcgccggc gataccgtgc tggtgttcga cgggctcacc
      661 ggggaggtgc gcgctgccaa gatcttcgtc gcggtcctgg gcgcatcgaa ctacaccttc
      721 gcccaagccc gcttcagcga ggcgctgccc gactggatcg gcgcccatgt ggatgcgctc
      781 gctttcctgg gtggggtgcc gaaggctctc gtctgcgaca atctcaaggc cggggtcacg
      841 gcggcgagcc gctacgagcc gggcgtcaac cggacctacc aggacctcgc gacccattac
      901 ggcagcacca tcatgccggc gcggccacgc aagccgcgcg acaaggccaa ggtcgaggcg
      961 gcggttctca tcgtccagcg atggattttg gcgcggctgc gcaaccgacg cttcttctcg
     1021 ctcgcagagt tgaacctcgc gatccgcatt ctggtcgacg aactcaacgc ccgcctgatg
     1081 cgcaagctcg gcgccagccg ccgcgagttc ttcgacacca tcgatcgtcc cgccctgatg
     1141 ccgttgccgg ccgagcccta tcagtacgcc gaatggcgtc gcgcccgcgt ggcgccggat
     1201 taccatgtcg aagttcaggg gcacttctac tccgtgccgt cccgcatgat ccgccaggtc
     1261 gtcgaggtgc gggccaccga ggctaccatc gaggtattcc atcgcggcaa tcgcatcgcg
     1321 agccacgcac gctcgggcgt gaagcaccgc cacaccacga tcccagagca catgccgagc
     1381 gcgcatcgcc gatacgcctt ctggacgccg gcgcgtctgc tggccgccgc cgagaagatc
     1441 ggtccatcca cggtagcgtt gtgcgaggcg atcatgcgga caaagcccca tcccgagcag
     1501 ggcttccgat cctgtctcgg cattctgcgg atggagaaga cctatggggc gcagcgcctc
     1561 gaagccgcat gccgccgtgg gatcagcatt ggcgcggcaa gctatcggtc gatcgcttcg
     1621 atcctcaaga ccggcctcga caaggccttc ctgcctgaga ccaaccccga tgccgatccc
     1681 atccagcacg gcaacatccg tggccgcggc tactaccact gacctgaaag aagaaggaga
     1741 ccctatgctc gtcaaccaca cccacgaacg tctcgtcgca ctcgggctcg ccggcatggc
     1801 caaggctttc gacgatcagc aacgccaacc cgacgttacg gcgctcacct tcgagcagcg
     1861 gctcggcctg atgattgatc gcgaagcgac cgagcgggag aacaaacggc tcgtcgtgcg
     1921 cctgaagttt gcctcgctgc ggcagaccgc aatcgtcgag gatgtcgatc tgcgcgcgcc
     1981 gcggggcatc gatcgcgcct tcttcgccaa gctcgtcgat ggcgattgga tcagccacaa
     2041 acaaaacttg ctcatcaccg ggccgaccgg cgtcggcaag agctggatcg cctgcgcgct
     2101 cggccacaag gcatgccgcg acaatcgttc cgtcctctac catcggctgc ccaggctgtt
     2161 cgaagccctg gctctcgccc gcggcgacgg acgctatgca cggctcctca agatgctgtc
     2221 gagggtcgat ctgctcatcc tcgacgactg gggcctcgcc ccgcttatcg gcgagcaacg
     2281 tcgcgatctc ctcgaagtcg tcgacgatcg ccatcagcgc ggatcgacca tcatcaccag
     2341 ccaggtgccg atcgaaaatt ggcactgacg ccgtcctcga ccggctcgtc cacaacgctc
     2401 accgcctcgc actcaatgga gacagcatgc gaaagatcac tgcgcagcgc gccaaacttg
     2461 acgcaaccaa gaaaatctga ccccactcca aatgccggca acgacacccc ggccgccttc
     2521 gtcggaacag gtggccgcct tcgatcggac cgcatggtcg cgttcaatcg gaatccatgg
     2581 ccgcaatccc cggaatccgc atgggtgaat tatgagggcc gctatttggc gggcaagctc
     2641 aaggcacgca ttgcgcagag gcccaccttc atggcgccac ctcccttgaa tcacccgtaa
     2701 cgatgttgct ggtattgtga cagatgctgg attgcggagc ggcaagtcgc cggcgaagct
     2761 cgccgtaaat ttcagctctc tccgcgggtc acgagttgga gccacttcca actcgcaaat
     2821 ttgtcgccag tctgacgcag gtggctatat cgcttcaagc tgctccagct cctgtgacct
     2881 gacacgctgg cggcttgcgg tatcgttttg cccatttcaa acaggcgcga aatcccgtcg
     2941 tgcctgagat cgtggaagtg cagatcatca attccgagga atttgcatgc acgcgtgaac
     3001 ccagcaccaa ttgcgtctac cgagtagggg aatatttctt ttgctacacg tggcatgctt
     3061 tcgataactg cagtcgcttc aggcggcagg tcacagaaca cgtcattccc ctgcctgtcg
     3121 cctggatgct tcatgtcgcg aatgagaaca cgagcatctg gatgagtggg cggcgaggca
     3181 gcctcaaaat ctgtccatgt gatccgcgtg atctcttcct gccgtcgcgt actgaaaatt
     3241 gcgaatacga tgatctgctg catcggggcg gaccgcggcc gatgagtttt gaccttgccg
     3301 aaatggtgca tgagaaggtc gagttcggtc aaggttggtc gacgatcgcg ctccttgctc
     3361 ttcgaagtga gaccgaggtt tctggtaact accagtgcat cgtccatcgc ctgtgagtct
     3421 aacggatagc cccaggccgg ccgtgcaata gtgaagattg agccgaggtg gctaaagtaa
     3481 tttccaacgg tttgtggttg aaccggcaat ttcttggcga aggcgactag gtgctcactt
     3541 gtcacctcgc tgcacttgag atccgctatg tcatagtcct tgatggtagc gagtacctga
     3601 gctttggttc ggccgatttc cttgatcgat tcgtcgatgt agcggtcgat caccgcggac
     3661 agcttcgggt ccggcatatt ctgccgctcc agcccgcctg gcttcgcaag ctcagtctct
     3721 cgcttctcca tccacgcgga agcagcttgt ctgcgatcga aggtcctcgt ctcgcggtgg
     3781 acgatttccc cgctacgctt aatggtgact tgcgcgtgat agccggtgga accgtctttg
     3841 cgcttgcgag caatgattgt acccattccg caaacccgcc agttgtggta cagcatggac
     3901 cttgtcagta cagcattgct gtaccgaagg ccaaaaaaca agcgaaaaac agcggcaatg
     3961 agacctgatc agtacagcag gaaaccgtcg aaagtgactg ataaaactca taaatatagt
     4021 aaaatcagta acttcgcgtt ttctgtagct cctatgatgg attggaccga ccggcattgc
     4081 cgcgtcttcc atcgtctgat gacgcggcgg gcgcggcttt acacggagat gctgacgacg
     4141 ggcgccatca tccatggcga ccgccggcgg ctgctcggtt tcgatgcctg cgaacatccg
     4201 gtggcgcttc agcttggtgg ctccgacccg cgcgatctgg cgaccgctgc gaaaattggc
     4261 gaggcgttcg gctacgagga agtcaacctg aacgtcggat gtccgtcgga tcgggtgcag
     4321 gacggccggt ttggcgcctg tctgatggcg gagccggcgc tggtcgccga gtgcgtccat
     4381 gccatgaagc gcgcggttga cattccggtc acggtcaagt gccggatcgg catcgacgat
     4441 caggatcccg aaattgcgct ggatgcgctg gcacgcggcg tgatcgcggc cggcgcggat
     4501 gcgctgatcg tgcacgcccg caaagcctgg ctcagcggat tgtcgccgaa gcaaaaccgg
     4561 gacgtcccgc cgctcgacta tgacattgtc taccggctga aggccgcgtt gcccgatatg
     4621 ccgatcatca tcaacggcgg catcggaaac ctcggcgagg ccaaggcgca tcttcgcttt
     4681 gtcgacggcg tgatgctggg gcgcgcggcc taccaggagc catggcggct gcttggcgcc
     4741 gatcccgaac tattcggcga agcagcgccg catccgacga tgaagcacgt gttcgaggcg
     4801 atggtcccct atatcgaaag cgaactcgcg caaggcacgc ggctgcattc gatcacgcgg
     4861 catttcgtcg gtgcgtttca cggcgtgccg ggcgcgcgcg cctttcgccg ccaccttgcc
     4921 gagaacggcg tcaggtccgg cgccggcgtc aacgtcttgc gcgatgcgat cgcgctggtc
     4981 gaggacagtg ttgtcgcccc gatcgccgca tgatgtcgaa accaaaaatt ttgtgcgtat
     5041 acggatcgca aaaccgaaaa tccgccttga tcgcgaagcc atcgccgaag ccgcgcgcgg
     5101 cgtacaaccg gaagatagct ggtggtcttc tgatggcggc ttttctattg aacctgttgc
     5161 agtcaattgg gcggagtcac cgtatagtcc gagaagtgca tgtagctgtc cgccttggac
     5221 cacagaccaa cccggcctga cacggcttcg ggaagcgtgt gctccagata gagcttgcca
     5281 tcgagatagc cttccacctt ggtgccggaa acgcgaacct taaggtcatg ccattgcttg
     5341 ctggcggccg gggcgttccg gatccaggct accgatgagc gtttgccttt ttcgaacttc
     5401 cagagaacca gattgttttc cagacaattc gcacgaagtg ccagataatc gccgtttggc
     5461 ttcagattaa acaatatgcc ggcgccctga tcgatgcggc ccgataatcc ttcgaaccgc
     5521 acggtgatgt caccgccacg aaaattttct acgtcattgg tgacagtgta agggtagtac
     5581 gcataggctt gaacccgatc gagaaattca gcatagcgtt cgccgtagag cgctcttgcc
     5641 ttatcggcga ttccggcgga ggactgccct tctttccatt gccgcccatc gacggccagg
     5701 acgtttttcc cgccttcgct ttcaatccgc cagaccccga cgacggccac aagcgactta
     5761 ggctccgctc cgaccgtctc gccagagaaa tcgattttgg tcggctgttc tgccgcgaca
     5821 ggcaatacca atgatatgag gccgagcgcc gaggctgcga tgacgctgat gcatttgatc
     5881 atctccatgg gtgaatctcc ttcatgatcg tgcggtgatt gggtagacgt cgattccacc
     5941 gccaggcgag ggcgatacag agtggaagtc cggcgaagaa ccaaagaaga gcgacccttc
     6001 tgccggccac cagcggataa ccgggataat cgctccccgg ggcaggcgcg ggaggactga
     6061 cccctgcgag gccgtacagc atggggagga tttcccgggg actggtcgag cggctgacgt
     6121 cagatcgcgc gccataggcg tattcgacct cgccgtagcg atcctcgccc gcagtggctg
     6181 cctggccttg ccgcccgctc gccagtcgga tcgacacatc aggcattgcg cgttcgagct
     6241 tcgccagcac gttgcgtcgc aggtcggcgt agcgggggtc ttcaggtacc agattaaccg
     6301 tcacgaccag gggctgcgcc aaagatgcga gcaggtgctg gtcggctgcg ggaaaagagt
     6361 tgcgtcgatc ttcggtgacg tcgaacgtga gtttgatctg cgttgcgagg ccgagcacga
     6421 gcgccacgac aacaacccac gcgatcgagc caatgacttt gtcgcggact tgaactcccg
     6481 gcggcaacca aacgtttgcc agcgccgcga aaccgaacat cgccgcgccg atacccagca
     6541 caagcccagc cgacaagaga ccttgctcaa acgtacgcaa tacctgggtc agcgacaggc
     6601 gggcaatcca tgcgagaaac ccgggttggc cggccacggt gaaatccagc acccatgaac
     6661 cgatcgtgaa ggcgagagta acgatcgcgg cagtcgctgc gctttccgac acggacgcag
     6721 caaacaaggc gatggcgccg acaagcagcc catagagcag gtgtccaaaa agcaggttca
     6781 acgtttccgg tgcggacaag tgcccaccct gcatgcccca gataaccagt gcggagaacg
     6841 cggggatgct tgaaagcacg agagcggcga gcaccgccgc gagcttggct gcaatcaagg
     6901 caggcggacg atagggcagt tggaccagaa gacgcagtgc acccgattcc ttttcctgac
     6961 cgagcacacg gatagcaaca aagggaaaga gcagcgtgat cgcgacataa gaggctccca
     7021 gggtcgggac caggacgccg tccaatggcg acaggctgct cgcgagcact ggtgattgca
     7081 gccccgccac actggcctcg ccgtacagcg aggtggcctg aaggaaactg tatccgagca
     7141 aagggcacat cagcagcagc atggtccaaa gcgcccggcc gctgaggatc tcccacagct
     7201 ccttgacaag cagtggccgc aagggcgcgt tctgaacgtg aaatgtttca ggtgagtgca
     7261 agaaagatat cctccaggct tccctcggaa attccggttc gggcgcgcag atcattcagt
     7321 gtccccacac cgcgaacacg accatcggca aggagaatga agcggtcaca gacgcgctcg
     7381 gcgtccggca attgatgtat cgcgagggca agcgtccgtc cctcgctggc ttctcgacgc
     7441 agcacgtcga cgatcgtgcg aatctgccgg aggtcaaagc catcgaacgg ttcgtccatc
     7501 agcagcagcg ggtggggcgt aagcaatcct agcgccaaca tcagccttcg gccgtagccc
     7561 ttcgacaggg agtgcacgcg cttatgaagg accggcgtca aacccaccga tttgatggca
     7621 tccgcgattt cgccggtcga tctgcgatag acgcctgcaa tgaacgatag gacccgagtg
     7681 acgggttgat ccaggtaagg tcgcacgcca tcaggcaggt aaaacatggc ttcgcgccgg
     7741 cgtgaggccg gcaggcgctc tccgcgccat cgaacgtcgt tggtttccgc ggggagaacg
     7801 cccgcgaggg cctcaagcaa ggttgtcttg ccggcgccgt tcggaccaat aataccgagg
     7861 atctcacctg ccgggacgtc gaaggcgaca tccgagagta ctgcctgatc cgcatattgc
     7921 ttcgtgatcg aatcgacacg aagcagaacc ggatcgcgcg cttgtccaaa agcggacgca
     7981 gctacgatcg ggagaactcc atccatgcgc aatctcctcg acctgggcga gtgttgcgca
     8041 gagcttggac ggcgagcgtc tgagggggag tctgccggtc cccaactaga ggttaatcct
     8101 ctatccgtcc gactgcaagg acccggtacg cgggctctgt cgacaatacc ccggctcttc
     8161 ggcttttgcg gaatacgcgg ctcagggata gccgtgcagc atcagcctgt cggcgcgcac
     8221 ttcccagctt tccagtcgat ccagaaagct catgccaagc agattggtct tcatctgtcc
     8281 gcgcggcacc accagcgccg gtattgagcg ctcgacgagc ttgccgatcg caagacgatc
     8341 gagcgtcagc cgcgccgcct tggtgtgtcc gccggcggtc tcgacgtcga catcatattc
     8401 gagcagctcc agcggcagcc cggcagcctt cgccgtctcc caggtcagta cgaccgaggt
     8461 cgcgccggtg tcgatcacca tcggggcgtt cgcgccgttg atcttggcac gcagcgcgaa
     8521 ctcgccggtc tgaccgcgcg ggatctgcac ggcgcgcgcc ggcgacgccg cccgcttgcg
     8581 catcacttcc gagacggtgt cgctcgcccg cgcgatctgt tccgggtcgg cataggcgac
     8641 cacggcaccg accgtcgcag cgagaatcag ggtgacaagc agaacgcggc tcatggctcg
     8701 cctcccgtgc aaatggtgtc gcaaatggtt tcgtaggtca ttcgggtttt ggaccgaccg
     8761 tcatccccgg ctgagccagg ccagcgttgg tcatccgcgc cgcgagttgc gccatcaccg
     8821 catggcgctc cgcgcttgcc atccggtgcc agcgcgcgat ctccggcaat gtacgtccgc
     8881 agccgaaaca gaggctggtt ttgggatcga tcatacagac tgcgatgcac ggcgtttctt
     8941 tgctcattgt gggagtgtga aatggtttcg gtggtttacg caagcgctcc agttacagtc
     9001 cggtgccggc actcatgatc ggtttgttgc gcgcccggcg ccgttcgtcg atggtggctt
     9061 ccggctgcag cggcggagcc tcctcggcca tcggcgccag cgcggacatc acgcgctcag
     9121 gcgggaaagt gacgatgacc tcggtgccga tgcgaagctt cgatttcagc gtaaatgtgc
     9181 cgccgtgcat gtcgaccagg ctttttgcga tcggcagccc gaggccggcg ccctgttcgg
     9241 ccgacttgat cgagttggag ccctggccga acgacgcgag cacgatcggg atttcgtcct
     9301 ccgcaatgcc ggagccggta tccttgacgc tcagatattg tccgcccgac gccgtccatc
     9361 cgactttcag ccagatctcg ccgccctggg gcgtgaactt gatggagttg gacaagagat
     9421 tgagcacgat ctgccgcatc gcgcgctcat cgccccaaat tcgcggcatg ccctgctcga
     9481 acacctcgtg gatggtgatg ccgcgactgg acgcgcgcag cttgagcaaa tgatggcagt
     9541 cggcgacgac gtgcaccagc gacaccgctt cttcgttgag ttcgtatcgt ccggcctcga
     9601 tccgcgacag gtcgagaatt tcattgatga gattgagcag atgcaccccc gaattgtgaa
     9661 tatcggccga atattccttg taaaccggga cggcgtgggg gccgaaaatc tcgctcttca
     9721 tcacctcgga aaacccgagg attgcattga gcggcgtccg cagctcgtgg ctcatctgcg
     9781 ccagaaaccg cgacttggcg acgttggccg attcggcgcg gtgccgggcc tcgtcggata
     9841 tcgacttggc ctgttcgagc tcgccgatca gcgcgtcttt ttcggcgcgc gcctccaatg
     9901 tcgccagcgt ggtcgaatgc agacgacggg cgagcagcgc aaaatagcct tcggcgatca
     9961 gcgcgagcac cgcgagcacg taatggtcga aggtaccgct cagcgcgaaa ttcagcgcga
    10021 tcgccgatgt gaccggtacg gttgccgcta gcgctgcgat cggcaggctt gccgcgagca
    10081 tgctcgatac cgcgatcacc agcagcatca ggaacatcat cagcgtgttc gagaccgcat
    10141 caatgctggc gggatgggtg aggatcgcgg tccagcacag cccgtacaat agatcgagca
    10201 gaacgaatcg cgttcgccat ttgcgggtgg cggtgagcga ggccggctcg ccaaggaacc
    10261 tgaggcaatt gcggatgatg gcggcatgaa tgcacagcat gccgcaggtc cacgccgcgg
    10321 ccgagaccgg atgcatccag aacccgaaca gcaagccggt cgcgaccacc agcagcatca
    10381 cgacatacga tgccgagatg cgggtctggg catgttgccg aagcagttcg cggtcgaagg
    10441 cggggcgggt tccgctggtc gacgttaacc tgtcgcgggc ctcgcgcacc cgctgcgcgg
    10501 ctgcacgacg gttattgacc ggcggtgcaa tcggcgtctc ggccggaagc tggaagactt
    10561 cgggcttatc ggcggggtta ctcatcaaac aacacaaatc ctgcccgcga acggcgcgaa
    10621 cttttgtatt gtctatatct ggcgccaaat cgttaagagc tgccttaaag agctaaagaa
    10681 tcacgttaac gggaggttaa aattaacctg ttcggcgggt cctgagagcc tgactgcgaa
    10741 atgcagccaa tagaatcggc gcccttggcg agactcgtca tgcccgggca gaagcgcgaa
    10801 gcgcgtcttc gcgctagatg tcccgggcat ccacgtctta acaggctagc agcgagcaag
    10861 acgtggatgg ccgggacata ggcgagcgga agcgacgccg tccttcggac ggctatgccc
    10921 ggccatgacg aaaaagcgaa tccttttcaa gttgtttgag agcattggaa atgctcgctg
    10981 catttcgagt ccgactctga ggtcagcgct ccagccgcgc caacaggctg gaggtgtccc
    11041 agcgcttgcc gcccattttc tcgacctcgg agtagaacgc gtcgaccagg gcggtgaccg
    11101 ggaggctcgc gccgttgcgg cgcgattcgg cgagacagat cgacaggtcc ttgcgcatcc
    11161 attccaccgc aaagccgaaa tcgaatttgc cttcgttcat ggtcttgtag cggttctcca
    11221 tctgccagga ctgcgcggcg cctttcgcga tggtgtcgat gacggcggtg atgtcgagcc
    11281 ccgccttttt ggcaaaatga attccttccg aaagaccttc gaccagcccg gcaatgcaga
    11341 tctggttgac catcttggtc agttggccgg agcccgccgg tccgagccgc ttgcacatcc
    11401 gggcataggc ggcgatgacg ggctcggcgc ccgcataggc gtcttcgctg ccgccgcaca
    11461 tcacggtcaa gacgccgttt tcggcgccgg cttgcccgcc ggacaccggg gcgtcgacga
    11521 atttgaagcc gcgcttggtc gcctccgcgt cgagttcgcg cgcgatttcg gcggacgcgg
    11581 tggtgtggtc gacgaaggtc gctgcctttt tcatgcccgc gaacgcgccg tcggcgccga
    11641 gcgtgatcgc acgcaggtcg ttgtcgttgc cgacgcaggc catgacgaaa tcctggcctt
    11701 ccgcggcggc cttcggcgtc gcggctgtct ttccagcgaa tttctcggcc caggcgagcg
    11761 ccttggccgg cgtcctgttg aacaccgtga cctcatggcc gcctttggcc accagatgtc
    11821 ctgccatggg aaaacccatc acgccgagac cgagaaaagc gactttagcc atgtttacaa
    11881 ccctggggtt tcgccggcgt tgctggccgg acctatgctg gaggaggggc ggcgggccgg
    11941 ttccgtcttc gaacaggttt cgccgaggat gcggggcgca caataaaccc tgcgtcctga
    12001 agggcaacgc ccccgtttgg cgggcggggt tggttttgtg ctaggatgaa gccaccgaca
    12061 tcgaggattt caagaaaaaa ggagtgacat cgcatgagcg tgagcgtagg cgtgctcgat
    12121 catttcaaca tccggacccg caagctcgcc gacacggtcc ggttctacga ggatgtgctg
    12181 ggcctggaga agggtgcccg gccgaacttt gcgttccccg gcgcgtggat gtacagcgaa
    12241 ggcaagcccg tggtgcatct ggtcgatatt tccaggaccg acgagccgca aaagccggat
    12301 tccggcgtcg tccatcacgt cgccttcgcc agccagggtt ttgccggcat gaggcagcgg
    12361 ttggagacca aagggatgga attcgactcg cgacaggttc cgggcggcga tctctggcag
    12421 atcttcgtca atgacccgaa tggcgtgatg atcgagctga attacgaggc cgccaaggag
    12481 cagagcgcag ccacagctcc gaccgagcgg gcggatgatg tcggcgtgag gtagcctttt
    12541 gagtgggacc gctctatggg tcgcatccga aaccaggtag gagatgcacc gtgagcgtaa
    12601 cgcagcaaca ggtccaggat gcgctggcca aggtggcgtc gccgcgcggc gtcgctttga
    12661 ccaacgccaa cgtgctgtcg gccatcacgg tcactgacgg caaagtgttc ttttcgatca
    12721 acgtcgatgc cgcggaggcc cgcgcctggg aaagcgttcg cgcccgggcc gaagcggcgg
    12781 tgcgcgccat tcccggcgtc aacgtcgcca tgatcgcgct caccgccgaa cgcaagccgg
    12841 gcgccgcggc ggcgcccccg ccgcatcggc acgcgcaggg ggtgccgcac gtctcgtcgc
    12901 acaagccacc gcaaagtccg gtgtcgccga tgtcgaagca gtcggaaatt cccggcattg
    12961 ccgccgtcat cgcggtggcc tccggcaagg gcggcgtcgg aaaatcgaca accgcgctca
    13021 atctggcgtt gggcctgcgc gatctcggct tgcgcgtcgg cctgctcgac gccgacatct
    13081 atgggccgtc ggtgccgaga ttgaccggca ttcgccagaa gccgcagctc aacgacgaca
    13141 agaaaatgat tcccattcag cggttcggcc ttgcgatcat gtcgatcggt tttctggtcg
    13201 aggaggagac cgcgatgatc tggcgcgggc cgatggtgat gtcggccatc acccagatgc
    13261 tgcgggatgt cgcctggggg acactcgata ttctcgtggt cgatatgcct cccggtaccg
    13321 gcgacgcgca actgacgctg gcgcagaacg tgccgctgaa gggggcggtc attgtctcga
    13381 cgccgcagga cctttccctg atcgacgcgc ggcggggact ggcgatgttc aagaaggtca
    13441 atgtgccggt tctcggcatt gtggagaata tgagctattt ccagtgtccg cattgcggca
    13501 cgcgctcgga tatcttcggt cacggcgggg cgcggcatga ggcggagcgg ctcgcggtgc
    13561 cgtttttggg cgagatcccg ctgcacatgt cgatccgcgt cacctccgat tcgggaacgc
    13621 cggtcgtgga gagcgagccg gacggcgcgc acgcggcgat ctaccgcgcg atcggcgcca
    13681 aggttcgcga tcagctccag ggcgttatcg ccgcggcctg agttgatttt ccgggggcat
    13741 gagactttcg tttaatcagt gctgtcatgc ctttgttgcg ttttcgcggc cggacttccg
    13801 tgttaaagcg cgcccgccag agcgcccttc cgcgaacgcg aagaatcgtt gggtccggaa
    13861 ctgctttagg aaatcggggg aaacgcccca acaaggagat gcctgaaatg aagcgtcgtg
    13921 attttttgaa agtgtcggca accggcgcgg cggtggccgc ggtggcctcg ccggcgatcg
    13981 ctcaatcctc gcccgagatc aagtggcgca tgacgtcgag tttcccgaag tcgctcgata
    14041 cgatttatgg cggcgctgaa tactttgcga aacaggttgc ggagatgacc gacaacaagt
    14101 ttcagattca ggttttccag gcgggcgaac tggttccagg attgcaggcg ctggatgcga
    14161 ccgccaagaa cacggtcgag atgtgccaca ccgtctcata ttattatgtc ggcaaggatc
    14221 cgaccttcgc gatctatgcg tcggtgccgt tcggcctcaa cgcccgccag cagaactcat
    14281 ggtggtatca aggcggcggc atggaaatcg gcaacgaatt tttcaagaag gccggcgtga
    14341 tcggatttcc ctgcggcaac accggcaccc agatgggcgg ctggttccgc aaggagatca
    14401 agacggtcgc cgatctctcc ggcctgaagt ttcgtatcgg cggcattgcc ggccaggtct
    14461 tgcagaaggt cggcgtggtg ccgcaacagc tcgccggcgg cgacatctat ccgtcgctcg
    14521 agaagggcac catcgacgcc gccgagtggg tcgggccata cgacgacgag aagctcggat
    14581 tccagaaggt cgccaaatat tattattatc ccggtttctg ggaaggcggc ccgacggttc
    14641 acgctttcgc caacctcgac aaatggaatg agttgccgaa gagctatcag gccatcgtca
    14701 gcaatgcctg tgcgaacgcc aacagctgga tggcggcgcg ctacgacatg cagaacccct
    14761 cggccttgaa gcgtctggtc gccggcggca cgcagcttcg tcccttcacc aacgaggtgc
    14821 tggaggcctg cctgaaggcg accaacgaat tgtgggccga aatatccgcg aaaaatcccg
    14881 acttcaagaa atcgatcgac gcgatgcagg cctatcgctc cgaccagtat ctgtggtggc
    14941 aggttgccga atataccttc gacagcttca tgatccgttc gcgcacgcgc ggctgataca
    15001 ccttaagtag cagtagtagc agttcgaaag cccggccttc gcgagaaagg ccgggctttt
    15061 gcgtcaagat gccgctaatt catcgtggca atcgggaacc ggatgttcca tgctgcccgc
    15121 aagcctcgac acgaaggctg gccgacaccc ccacaggagg aaatgatcca atgaagcgac
    15181 gagactttat caaggtcact ggcatcggcg tcgcgggcgc cgcgaccatg gcggcacctg
    15241 cgatcgccca gtcgacgccg gagatcaaat ggcggatgcc gacaagctgg ccgaaatccc
    15301 tcgacaccct ttatggcggc gccgagacaa tggccaaggt cgttgccgaa gccaccgaca
    15361 acaagtttca gattcagacg ttcgccggag gcgaaatcgt accgggactg caggttctgg
    15421 acgcggtaca gaacggcaca tgcgaaatcg gtcacaccgc atcgtactac tacttcggca
    15481 aggacccgac cttcaccttc ggctcgtcgg tgccgttcgg acccaacatg cgcatcaatc
    15541 aggcctggta catgctgggc ggcggcaaag aggtcctcaa cgagttctac aagaaatata
    15601 acgtgacgtc gctgctcgcc ggcaataccg gttgtcagat gggcggttgg ttccgcaagg
    15661 aactcaccag cgtcgacgac ctcaagggtc tcaaattccg catcggcggc tttgccgggc
    15721 gcgttctgca gaagatgggc gcggtgccgc agcagattgc cggcggcgac atctaccccg
    15781 cgcttgagaa aggcacgatc gacggtgcgg aatgggtcgg tccttacgat gacgagaaac
    15841 tcggattcta caaggtcgcg ccgcactatt actatcccgg gtggtgggaa ggcggaccga
    15901 tgctgctgtc gttcgtcaat ctcgataaat ggaatgcact gccgaaatac taccaaagcg
    15961 tcctcgagca ggccggtcac tatgccaaca actggatgat ggcgaagtac gaccagtcca
    16021 atcctcaggc gctgcgccga ttgctcgcga acggcaccaa gctgcatgcc ttctcgccac
    16081 cgatcatgga agcttccctg aaagcggcga aggaattgca cggcgaggtc gccgcgacca
    16141 acgcggactt caagaaggtc tacgaatcgc tgaccgcgtt ctcgagcaac agctaccagt
    16201 ggttccaggt cgccgaagtc ggttacgaca acttcatggc gcgtcactcc cagagctgat
    16261 ggccgcgccg accattgggc caacgaagaa aaaaccccgg cgcgaatgtg ccggggtttt
    16321 tgtcttgcga aagcacaaat tgctattgtt ttggcggcgg cccgaaatcc agcggcggaa
    16381 gctcgatctg cgggatgtcg atcttgatgg tgttgggatc aaccgtcgac agagcgcctt
    16441 tgtaatgcat caccatcgac gggaacatga tcaccagcag caccatggcc acctggatga
    16501 cgacgaacgg caccgccccc cagtagatct ggcccgtggt gacggggtcc atgcgcttgc
    16561 cggtgacgcg gtcgaggtag gaatccttcg gcgccaccga gcgcaggtag aacagcgcga
    16621 acccgaacgg cggatgcatg aacgaggtct gcatgttgac gccgaggatc accccgaacc
    16681 agatcaggtc gatgccgagg tgttcagcgg ccggtccgag cagcgggatg acgatgaagg
    16741 caagttcgaa gaaatcgagg aagaaggcga gcacgaacac gaaggcgttg acgaaaatga
    16801 gaaacccgat ctggccgccg ggcagggagg tcaggagatg ttcgacccag acgtgaccgt
    16861 tgacgccata gaaggtcagc gagaagaccc gcgccccgac caggatgaag accacgaagg
    16921 cagatagttt tgcggtggat tccgtggcct gccggatcag gtcccagctc agccggcgct
    16981 tggcggcgcc gagaatgatg gcgccggcag cgcccatcgc gccaccctcg gtcggtgtgg
    17041 cgaggccaat gaagatcgtg cccagcacca ggaagatcag gaacagcggc ggcaccatca
    17101 cgaaagtggt ttgctgcgcc atcgcggaca ggaaccggaa tcccgtaaac ttgtcgatga
    17161 tccaattcag gacggcgacg aagaacgcga acaggatgcc gaagaacatg ctgagcacga
    17221 cgaaatcggc gccgtgggtt tcggagctcc gcatcatgaa ccagccgaag aaacagctgg
    17281 cgatgaacag catcaacagc gaaatcaatc cgcggctgcc gtctttctcg cgaaagccga
    17341 tcgcctccgg cggcaggccc ggagccgcct tgggaaatac cagtgtgacg ataaacgcgt
    17401 acacggcata gaggcccgcc agcaccaatc ccggaacgaa ggcgccctcg tacatgtcgc
    17461 cgaccgactt gccgagctgg tcggccatca cgatcagaac cagcgacggc ggaatgatct
    17521 gcgccagcgt gcctgaggcg gcgataatgc cggaagccat gcggcggtcg tatccgtagc
    17581 gcaacatgat cggcagcgag atcaggccca tcgaaatcac ggaagccgcg accacgcccg
    17641 tggtggcggc gagcagcgcg ccgacgaaca ccaccgcata ggcgaggccg cccttgatgg
    17701 tgccgaacag ctgcccgatg gtgtcgagca ggtcctccgc catgccggat cgttcgagca
    17761 ccagtcccat gaacgtaaag aacgggatcg cgagcagcgt atcgttgttc atcacgccat
    17821 agacgcgctc cggaagcgcc tgcaggaagt cgggccggaa ttcgccaagt tgaatgccga
    17881 caaaggcata gatcagtccc accgcgccaa gtgagaacgc tgccgggtaa cccagcagca
    17941 ggacaacgac cagcgacgcg aacatcagcg gcgccatatt ggcgatcaga aaagcagcca
    18001 tccagaatcc ccctgcgacc gcgtccgcga tcagtctttc ttgatcgcct cgacgagatg
    18061 ttcgacctcg gcctcgagag cattaacttg cgatgcgtgc ggatccggga tgagcccgcg
    18121 catcaccgcg atgcgcttga tcagttcgga tatgccctgc accagcagca aggtgaatcc
    18181 gacgatgatc agcgacttgg ccggccactg cggcaggccc cccgcgttgc cggattgctc
    18241 gttgatctcg accgagcgca tgaagaacgg accgccggtg atgatcatga tgatggtgag
    18301 cggcagcaga aaaaaagcgt gaccgataat gtcgatgctg ttgcgcacct gcttgggcat
    18361 catgttgttg acgatatcga tccggatatg ctcgttgtcg agcagggtcc aggacgagca
    18421 cagcaggaag acgatgctga acagcaccca ttgcagctcg agccaggaat tggacgaggt
    18481 gtcgaaggtc ttgcgcacga tcgcgttgag cgcggacacc accacggcga ccaggatcag
    18541 ccatgccagg cgtttgccca tccaccgcgt gaacgcgtcg attccacggc tcaatttcag
    18601 aagcgattgc aacgatcgtc ctcccccgat tgcgcttctg ccgtcttgac cgcgcccgtc
    18661 ggcgcagcgg cttatccagc cggccagcat gaagccgccg caccaaacca gcgcctgacg
    18721 ccgccgtcaa ttggaagttg gttgatattg aaggggaatt tggccccgcg gacgactgtt
    18781 gctgggcgcc ggggcggaaa cctcagccgc cggtgacgct catatgccgg ctgacgctcg
    18841 ggcggttgtg ccggcgatcg atgatgaagt cgtggccctt cggcttcagg ccgatggcgc
    18901 tgtcgatcgc cgagctgagc agatcgttgt cgacggaggc ccgcagcggc ttgcgcaaat
    18961 cggaggcatc ctcgtggccg agacaggtgt gcaacgtgcc ggtgcaggtg atgcgcaccc
    19021 gattgcagga ttcgcagaaa ttatgggtca tcggcgtgat gaagccgagc ttgccgccgg
    19081 tctcgctgac ccgcacgtag cgggcggggc cgccgctgtc ttcgtcgagg tcggtcaggg
    19141 tgtattgctt ggcaaggcgc gcgcggagca gggacaacgg gacgtattgg tcgatacgcc
    19201 cctcgccgat gtcgcccatc ggcatgacct cgatcagtgt cagcgccatg cccttgccgt
    19261 gcgcccattg catcagcgaa ggaatttctt cctcgttcag atttttcagc gcaaccgcgt
    19321 tgatcttgac ggcgagaccg gccgatcgcg ccgcctcgat gccggtcaga accttgtcga
    19381 gatcgcccca acgggtgatg gcgcggaatt tggcgggatc aagcgtatcc agcgagacgt
    19441 tgatgcgctt taccccgcag tcccttaatt cgctcgcaaa acgcgccagc tgcgagccat
    19501 tggtcgtcaa ggtcagttcc ttgagcgcac ctgtatcgag atggcgcgac agcgagcgca
    19561 ccagcgacat cacgttgcgc cggaccagcg gctcgccgcc ggtcagccgc aactttcgca
    19621 cgcctttggc gatgaacgcc gtacaaagcc gatcgagctc ctcgagcgtc agcaaatcgg
    19681 ccttgggcag aaatgtcatg tcttcggaca tgcagtaaac gcagcgcaga tcgcagcgat
    19741 cggtgaccga tacccgcaaa tagctgatgg tccggccgaa cgggtcggtc atggcacgcg
    19801 acagcgacgt gggaggacta accgggggtc cgttcattca atgtgccttg ttactgtgac
    19861 agccgccatg atggataggg cggaatggat ggcgcggacg gttccgcgcg atcgcaatgt
    19921 atgcatgatg cgcggcgcat acaatccgca ctttttctgc tgcagtgcaa gcatttagcg
    19981 ggtggtcggg gcgggcgttg gcggcggctc cggcgctccc gcaggggccg cggcggcctt
    20041 gggcggtttt ggctttttcg gcgtcaggat tttcttgccg gcggcccttg gcggaggagc
    20101 ggccggctga agttccgcga caaccggatt gggatcgacc gtggtcgacc ccggactggc
    20161 gaaatctccg gggatacgga tgacctgcac cgggacggtc gccggctgga acttgttcaa
    20221 cgtgtaggcg accgagaaac cgctgtccgg cgcgctcaca gcgaccgcgc acggggtctt
    20281 gcaacccggc cccagcgacg tccgcgcatc ggcgcctgac ggcactgaat cgagctgtac
    20341 ttgcaccgtc ggcggcgttg gtttgaaggc atccagggaa gcaaaagaag agcagccggc
    20401 caggctgtac cccgttactg caatcgcgat aacacgacgc atgatcaatc cgccactcta
    20461 gtgcggccac ccgccacagc ccgcaaaacg tcagcataag tgcgtcacac cagccgtgca
    20521 accgttggga ggtgaatggt taagcaatgg ttaatgcatg catttagcga ttttgatata
    20581 gccataaaat caacggctta gctggaattc cgcggaaaca tcaggctttc tacggcagcc
    20641 ggcaggtccc tcatgtgatt gatgtgcaga tccggcttga ggtcggcgat gggaacgtcg
    20701 gtgtagccaa attcaacgcc gatgacgggc actccggcgc gccgtgcgac gccgatatcc
    20761 ggaccggcat cgccgaccat gatcgctgcg gacagctggc cgcctgcgcg cgcgatggtc
    20821 tgctgcagga tcgccggatc gggttttgag acgccaaagg tgtcggcgcc gcaaatcgcg
    20881 gaaaatcgcg agctcaatcc gagccggtcg agcaggagct tcgacaacca ctcgagcttg
    20941 ttggtgcaaa ccgcaaagcg atatccgcgc tccctgagat catcgagcgc gctttccagg
    21001 ccatcgaacg ggcgcgaggc gtcggcgatg tgagctgcgt aataatcgat gaaatcgctg
    21061 gtgagccggg tgatgtcttc ggggccgacg gagcggccct ccagctcgag gccgcgctcg
    21121 atcagccggc gggcgccggc gccgatcatg ttgcgcgcgg catgcagcgg gaccggtggc
    21181 aggccctcgc ggtcgagaac ataattgagc gcgttgatca ggtcgggcgc cgtatccaca
    21241 agcgtgccgt cgagatcgaa aacaatagtg cggggagaag tcatcgaaaa ccgctatcgg
    21301 tcctcgcgca atcatgcaag gtgggacgac aggtgactgt gaaaccctgc gtaagggccg
    21361 ggactatccg cctgacgcgc ggctcgccag ccgcgaaagt cgtcaattgc ggccaaatcg
    21421 ggcgtatggt gctgttcctc cccgcgaaac gacgctatat gcccgccaga catcgccggt
    21481 cgcgggatcg gcattggatt tttcggggca tcgtttgcac gtgagcatgg acggactgaa
    21541 acggcaggcg gcgggccgcg cgctcgaatt cgtggccgac ggcatgaagc ttggcctcgg
    21601 caccggttcg accgcgaagc attttgtcga gctgctgggc gaacgggtcc gcgccgggct
    21661 tgacgtcatc ggcgtgccga cgtcggaggc gacccgcgcc gatgcggtgc gctgcggcat
    21721 tccgctgacc acgctcgacg atatcgatcg gctcgatctg acggtcgatg gcgcggacga
    21781 aatcgattcc gggctcaacc tcatcaaggg cggtggcggc gctttgctgc gcgaaaagat
    21841 cgtggccgcg gcctctgatc gcatgatcgt gatcgccgat gattccaaat gggtcgaggt
    21901 tctcggccgc tttccgttgc caatcgaggt cattccgttc ggcctggcgg cgacacggcg
    21961 ggccatcggc ggggcatttg cggaaagcgg cgtttccggg caaatggtgg tccgaaagac
    22021 tggggccggc cacgtttttg tcaccgatgg cggccactgg attgtcgatg cccatcttga
    22081 gcgcatcagc gatgctccgc atctcgcgcg attgctgaac tcgataccgg gcgttgtcga
    22141 gcatgggctg ttcatcgatc ttgccagcac ggcgatgctg gcgggctctc agggaattcg
    22201 tgttgttgaa cggcggtaac gccgcaatct tatggagatt aggaatgaag cgccttttcg
    22261 caatcttgtc ggccgcgggc cttgtgctgg gactggcgct ggccggcctt cccgcaggcg
    22321 cccagcaacc ggcggcgccc gcgctcaagc cgggaactcc ggcggcgatc gcggcggcca
    22381 aggaaattct ggccatgaag aatgccggcg cgatgtatgc gaacgccatt cccaacatcg
    22441 tccagcagac caaggacgtc ttgctgcaga acaacctcaa ctatcagagg gatctcaatg
    22501 aagtggcggt gattgtcgcc caaggcctgg ccgggcgcga gaaggagatc ggcgagggca
    22561 tggccaagat ctatgccaac gaatttaccg agcaggagct ggtggccctg gtcacgtttt
    22621 acaagtcgcc gctcggccag aagctgctgg cgagcgagcc ccgggcaatt cagttcagca
    22681 tgtcctatat gaaccagtgg gcgcagggtt ttgccgaggt cgtcaacggt cagtttcgcg
    22741 ccgagatgcg caagcgcggc aaggaaatct gacgggcaaa tctgacccta gaatccgatt
    22801 tcggtcgggg tggatatggc tgagttcgac gtcgatctgt ttgtcatcgg aggaggttcg
    22861 ggcggggttc gcgccgcccg gatcgccgcc ggctatggcg cgcgggtgat ggtggccgaa
    22921 gaataccgga tgggcggcac ctgcgtgatc cgcggctgcg taccgaaaaa gctgttcgtg
    22981 atcggatcgc acatccgcca cgagatcgag gacgccgccg gctttggctg gttgatcccg
    23041 ccggtgacct tcgactggcc gacgctggtc gccaacaagg acaaggagat cgcccggctg
    23101 gagggcatct acaccagcaa tgtcgaaaag tccggcgcgc gcatcgtcaa gacgcgcgcg
    23161 gtgttcgagg atgcccacac ccttcgcctc gccaccggcg agaccgtgaa ggcgaaatac
    23221 gtgctgatcg cgaccggcgg cgcgcccaat cacgggccgg cgatacccgg catcgaacac
    23281 gtgatctcat cgaacgaagt gtttcacctg aaggaattgc ccaggcgcat cgtgatccag
    23341 ggcggcggct atatcgcgct cgaatttgcc tgcatttttg ccggcttcgg caccgacgtg
    23401 acggtgatct atcgcggcga caacattttg cgcggttttg acgaggacgt ccgcgcccat
    23461 gtccgcgccg agatggagcg gcaaggcatc accattctga ccggctgcac ggtcaacaag
    23521 gtcgacaagc ccggccggga attcatctcg catctgtcga acggttccag catcgcttcc
    23581 gatctcgtga tgttcgcgat cgggcggcat cccaatgttg ccggcctggg gcttgagcac
    23641 gccggcgtcg ccatcaatcc caacaatggc gggatcgcgg tcgatggctg gtcgcagacc
    23701 tcggccccga acatctacgc gatcggggat gtcacccatc gcatcaacct gacgccggtc
    23761 gcgatccgcg aaggccacgc cttcgccgat accgtgttcg gcaagcgtcc ggttcaggtc
    23821 gaccacacca acattcccac cgcggtgttt tcccagccgg aggtcggcac cgtcggattg
    23881 accgagagcc aggcgcgcgc gcaattcagc cacgtcgata tctacaagac cgattttcgg
    23941 ccgatcaagt cgaccatgtc gggccgcgat acccgcatcc tgatgaagct cgtggtcgat
    24001 ggcacgaccg atcgcgtggt tggctgccat atcgtcggcg acaccgccgc cgagatcgtg
    24061 caggccgtcg gcattgccgt caaaatgaaa gcgaccaagg ccgatttcga cgccaccttc
    24121 gcgctgcatc cgacggctgc cgaagaactc gtgaccatgc ggacgccgac ggcgcgctat
    24181 gtgcgggagg cggccgcgga gtaacgcagc gccagggagc agacatgacc gccggttttg
    24241 tccagacgta ccgcgcattc gcctacaata acgcctgggc gaaccaccgg ctgctcacgg
    24301 cttgcgcaaa cctgaaccag caggcgtttg aagccgagcg gaccggcttc ttccctagcc
    24361 tgcagaagac gttgaaccac atttacatcg tcgacctgtt cattgtcgac gcgctggagg
    24421 gtggatggct cgggccaaag gcttttgcaa accgcgttcc atgtccatcg cttgctgaac
    24481 tcaggcgtgc tcaggccgcg gtcgacaagc gcctgatcgc cgtctgcgat acgctgacgc
    24541 cggacctgct tgatggcatc gtgcgggtca atcgcgatcc gcaggtacag accgaacggc
    24601 gcgaccgcct gctcatgcat ctgttccagc accagatcca ccatcgcggg caggcgcacg
    24661 cgatgctgtc cgcaaccgcc atcaaaccgc ctcagctcga cgagttcttt tcgatcgcgg
    24721 gagcatcgcg ccgggccatc gagttcgacg gtcttggctg gaccgaagaa acggtgtggg
    24781 gaccatgacc cgcgctgcgg gccagagcgt tttcaagcga gtggagaccg gttcgcgtca
    24841 agaaaacgcg tccaatcaag aatctaaagc cccgtttcga ttctatcgga aagggctcta
    24901 gttggctggc gccttgggtg ctgcttcgag cgtcgatgtg atttgtcttg cgctcgacaa
    24961 tgcccttcgg atgtttgggt cttccgaacc gaactcgaag gtgatcagcc ggttgcaatg
    25021 ctggcagttg acctgaaaac cgggcctgac cttctgcgcg cgcacccgaa acatctggga
    25081 gcatttcgag catttaattc tgaccttgtc atcgaccatg aaatccacct tgaagcccac
    25141 cctctcatgc ggaccttaag cacctgctaa ttttgcctgg aagcgacgct ctggaatacg
    25201 tgctacggct tcaccgatca ttatcgtccg ggtgcaccca gagataaggc gcggtctgcc
    25261 gttgttccgt cagcctgatc atgtgcaccg gcggcgaata gcgcggcgtg cggcacgatc
    25321 ggcatttcag cgcggcctcg agtttccaga tcggcgtctc gcgcggccgg cggacgtgtt
    25381 cgagcggaat gctggcctcg gtttcgcagc ggcggcattg cacctgcagc cagccgtacc
    25441 cgccattgag gcattgcgcg atggtcggcg agggctgcgc cggcccgcca tatccctcca
    25501 tgcggatcga ccacgcctcg gcctcggcgc gatcggcttc gcggatcgcc tttttggctt
    25561 cctgccgcgc gccatcggcg cgaatcttcg cgcccctcag ccgggagccg aagatggact
    25621 gtcgcgatct ggtgctcatg gcgcgccatc tcaccggcct ttgcgagcca acgcaatccc
    25681 actaggattt gcggtgcgga acagccgagc ggcgctacag atttggtgac atgtgcaact
    25741 tgtattccat taccacgaac caggccgcga tcgccgcact gttccgggtg atcaatcaat
    25801 acgtcggcaa cctgccgccg atgccgggcg tattcccgga ttatccggcg cccgtggtgc
    25861 gcaacgcagg ccaaaacacc gacggtgagc gcgagctgac catgatgcgc tggggcatgc
    25921 cgccgccgcc gcgcagcggt ggcccgcccg tcaccaacat ccgcaacacc tcgtcgccgc
    25981 actggcgcgg ctggctgaag ccggaacacc gttgcctggt gccggcaaac agttttgccg
    26041 aatacgcgcc ggagccgaac cccgagacga aaaagaaaga cgtggtgtgg ttcgcgcgcg
    26101 atgatgatcg cccgctgttc gcattcgccg gaatctggac ggagtttcga ggcgatcgcg
    26161 gcaccaagtc gaagccgctt cccggaccgc acctggtcta cggctttctc accacggcgc
    26221 cgaatagaat cgtcgagccg atccacgaca aggcgatgcc ggtgattctc accacgccgg
    26281 aggaatacga cgcctggctg cgcgcgccgt gggacgaagc aaaagcgctg cagcggccgc
    26341 tgccggacga ggcgctgaag atcgtggcgc gcggggcgga gaaggaggat gtcgccgtgt
    26401 ccggttagcg cggcgttgcg accggttcgt cgatcggcct atcggcgccg tgcgacggcg
    26461 acgcccagca ggaatgccac cagcagcgac gccagcggcg cctcgcgagc gatgttgctc
    26521 agaatgctca atggcatgcc cggctttctg cccgcgtcga tggcgtcgcc gatccggttg
    26581 gcggtgcctc tgacggcctc cgacacttcc gcgatggtcg atttgggctc cggtgcggga
    26641 tcgaggcctt cgtggacaaa gggtggggac tcgtcgctca tgatcctgtc ctcgcgcatg
    26701 tggcaatgcg gcatcccgat tggcttgcgg gttgccgtta cagcgatcca atgatcccgg
    26761 ttccaattgg ttccgccggc cagtgccgat gtgacaccgc gagggtcgcg tcgcaagaca
    26821 gcgtggcccg gatgagggaa gcgacatccg gtcgcgcgaa tgcgcgctcg atgataaact
    26881 ccgcgcaatc agccgttcgc cgacgacaag gaggcgggtt aagcctgcgg ctatcgcaag
    26941 gcattgcttg acaaccgtaa gccgttgcct tgcaaagcgg gcatgatccg ctcgttccgg
    27001 gacaaggccc ttggacaagg cccttgaacg gtttttcgcg accggtgacg ggcgccggct
    27061 gagcgttcag aatacgaaac ggatcgccaa cattctgcgg tcgctcgacg acgcatcacg
    27121 tcccgaggac actgaggact gattcatgac cagaaagctc ccgaagcgcg gcttgccacc
    27181 catgcatccc ggcgaacttc ttcgcgagga gattttgcct gcgcttgatc ggtccaagac
    27241 ggagatcgcc aaattgctcg gcgtgtcgcg tcagacgctt tatgatattc tcgcggaaaa
    27301 gcagccggtc acccctgcca tggccttgcg gctcgggaag ctgtgcggaa atggtccgga
    27361 tctctggctc aatctgcaga agcgatacga tctgcaccag gcgcagcagg aactcggaga
    27421 gacgatcaag aaaattccga cactggaagt cgcctgaaag cgaggatttg cttgacgccc
    27481 cgctcattcg ggctggacgc gccgttctga aagccgagtg ggaaaaggta aaggccgaaa
    27541 tgcagggcgg tccatttcaa tcagggattc cggctgaccc ggcgcgttcg agcctgcgcg
    27601 ccgaagtcaa cagccttttc cgccgcgacg tgatcggtaa gctgccgtag ggcctacggg
    27661 ctgctagatg ccttggtcag atgccttggt cagatgcctt ggcccgccgc ttcctgggcg
    27721 gcttttcctt tgtcggagcg ggaggtggga cgtttacgac gtcgatggtg ctcaacgatg
    27781 ccaaaacctt ttgtgacttc gatggctcga cttgtggggg cgattgttcc ggcacggccg
    27841 gaggtctatg catttcctcg tgttccggca cggctgaagg cgcatgcatt tcctcgacgg
    27901 gatgatcggc ttcgtaccac tcgatgactt cgaaggttat tccccgcctg agcatctccg
    27961 cctcgagatc cgcagcatga agcttccacg ccggtgtctt gcgaacgcga aacctgcctt
    28021 ctccgccaat ttccccgaat gcctcatcag ccgccagcgc ctcgcggacg ccttcataca
    28081 tcttgacaag gctgtcgtcg gtgaaccctg aataatccat cgggtctctc gctgcaaaga
    28141 acacgctaca taacaacggc ttcgacactg cgctcgcgct tcggctcctt ccagcgaatc
    28201 cgcgcggtaa ccttgatcag ttaggttacc ccggacaggg cgatgccggt gatcctgacc
    28261 gcgaaagagg aatacgacgc ctggatgcac gcgccatggg atgaagcgaa ggcgctgcag
    28321 cggccgttgc cggatgaggc gctgaagatc gtggcgcgcg gggcggagaa ggaagatcgc
    28381 gcggcggggt gaagaaagcg cggcggacta gatgttcacc gacggtaaag gcgggttacg
    28441 ccggagcctg tcatcgggcc gcgcttggcg cggacccggt gggctaaccc gcccgggcca
    28501 gttccttccg gggactggaa gccgtcgcat gctcggagcg ccacatcgcg atgatggaag
    28561 cgaggatgag gccggcgccg aatatcatcg ccgtagagaa tgtttctttg agaaagatcg
    28621 tgccgagtaa aatagcgacc accggattga cgaaggtgta ggtggaaact agcggcgttg
    28681 cgacgttgtc taacagccag tgataagcgg caaaaccgat cacgctgccc gccacgacga
    28741 ggtaggtcac cgctcccaga gaggtcgcag tgaccgcttg cgatgagaat tgttttactt
    28801 cgcccgtaac ggagctgatc gcaagaagta ccgcgccgcc gatcaccagt tgcatccctg
    28861 aaatgagtgc ggatgaaccg tcgctggccg tgcgccgcga ccatacagtt ccagcggacc
    28921 aggacagcgc cgcgccgagc agccacaaga taggaagaat gctgacgccg ttctcgccga
    28981 tattctgcca ggcaacgaag gcgacaccgt agaagccggg aaccagcgcc atcatcgtga
    29041 gaggagcagg ccgtctctcc tgcggtaaca ggaaatcaag caacacaatc cacaacggaa
    29101 tcgcggcgag gacgatggcg gcgacgccgg atggaaccgt ctgttggacg aaggcgagaa
    29161 cgccgtggca gccgacgaaa aacattagcc cgctcaagcc cgcgttcagc cacgttcgcc
    29221 gcgagtgctg aatgatttcg cgtccgcaca gtgcgactag aatcaagcca ccgcaaagcg
    29281 agcggacagc catcaataga aacggcggga acgacttcag ccccagcgcg atcgcgaaat
    29341 aggtcgaacc ccaaagcagg taaatggcgg caaacgaacc gataatcaga aatttcctga
    29401 ccataaccgg attgcactct gtacgatcgg cccagcatca actatctaac cctttaaggg
    29461 gttgtacgtt ccagggaact tgcacgtggc atcaatttcg aactctcccg ggaagcttgc
    29521 ccgcgaggaa ctctgtatca agtttgccaa cacggtcgcc tggcggctac gagatccagt
    29581 cgaggagcgc gccgcttcac cgcaagcaat gctggattgg ttggcgggtg ccggcctggc
    29641 cgatgggcaa atggtccgag aagtaacgac gcgatggaaa caatccccgc gagatgcacg
    29701 agttttcctc aaggaagcgg tcgagcttcg cgaggcgatc tactcgctct tcgtcgcgaa
    29761 gatccatgcg cggagagcgg atcctgtggc ggctgcaatt ttgaacgcgc accttgccgg
    29821 tacggtgcgt gggcttgggc tgacttcatc gcggacgacc cttaaatggt gttccacgtc
    29881 tcgaaatgtt gcgccggagg ccctgctcaa accgatcgcc tggtctgccg cgggattgtt
    29941 gatgggaccg cgtgcgcaaa aggtccgaca gtgccaggac gagcgcggct gcggatggct
    30001 cttcatcgat gaaagccgag cccaaaatcg gcgatggtgt tccatgggcg actgcggaaa
    30061 tctagccaaa tcgcgccgtc accatcgccg cgtatcggcc aaatgacgct gtctgagcaa
    30121 gcgtagcccg gatgagcgaa acgatatccg ggattttcaa atttccttta aaccccgcat
    30181 atcgctacgc catgcgggct actcgtcaag tttctatata agtgtagttg taccgatggt
    30241 cgccgacgaa gtagaagcgg gcatcgcttc tatccattcg caaacaaaat ccagtgacgt
    30301 gggtccgatt aaaaatgtcc cggaatctca cataacctat aaaaatcagg ttgtcgtctc
    30361 cggatgtgac tctcaagaac tcttgcttgt cgatccctct ggatggctct cttgtcgagg
    30421 gaggggaagt gccaccattt gctccaacga t
//
LOCUS       DATNOI010000324         3830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_718927,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000324 DATNOI010000000
VERSION     DATNOI010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718927"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            592..1119
                     /locus_tag="VN968_12870"
     CDS             592..1119
                     /locus_tag="VN968_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXL30166.1"
                     /translation="MLKLKNLARGNVTPSRTALAVFAATLLFGGSVTEASARSHRHHH
                     RHHHHHHHHASSTPSAAVGGSVGGSWLNANAAIAPSSGRSFSGMASYYGNESGSRTAS
                     GQRMNANAMTAAHRSLPFGTKLRVTHGGRSVVVTINDRGPFIRGRVLDLSTGAARVIG
                     LTGAGVGRVVAEVVS"
     gene            complement(1376..3586)
                     /locus_tag="VN968_12875"
     CDS             complement(1376..3586)
                     /locus_tag="VN968_12875"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXL30167.1"
                     /translation="MDHAIRASPSSASSARVVDVLVPVALNQAYSYRVPRGVELKPGD
                     VVCVPLGPREVVAVVWAENANPDPRLHNRLKDVGEKLDVPPLKEELRSLVDWVANYTL
                     SPRGMVLRMCLRMGEHLGPERVRMGVRLIGATPKRMTPARRRLIEVLSDGLLHGKSDA
                     AREAGVSTGVIDGLVDEGTLATEPMPPAQAPPAPDPSFAEPDFSRQQRTAVDAMRALA
                     ANGSFHVALLDGVTGSGKTEVYFEAIAEVIRRGKQSLILMPEIALTGQFLDRFARRFG
                     VRPLEWHSELTPRTRQRNWAAIAAGEAPVVVGARSALFLPYANLGLIIVDEEHDQAYK
                     QDEGAHYHARDMAVVRAHIAKIPIVLASATPSVETEVNARKGRYQRVVLPARFGGQHM
                     PHIEAIDLRREAPVRGRFISPRLAEHVQIAIERREQALLFLNRRGYAPLTLCRTCGHR
                     FACTICDAWLVDHRFRQRLVCHHCGFSMPRPHVCPHCAAEQSLVAVGPGVERLQEEAA
                     QLFPDARTMVLSSDLITSIETMRTELNEIAEGRVDIMIGTQLVAKGHNFPRLNLVGVV
                     DADLGLSNGDPRAAERTFQLLNQVIGRAGRDQGRGVGYLQTHQPEHPVMKALVACDRE
                     AFYASEIEARERAGYPPFGRLASLIISAGDRPTAESFARRLAATAPIDERIKVLGPAE
                     APLAVIKGRYRFRLLVKSLRNVDLSEYLREWLAAGPKTKGNLKLEIDVDPQSFL"
BASE COUNT          729 a         1243 c         1150 g          708 t
ORIGIN      
        1 gaattcaagc gggacttcga ttttcggtcg gtacgacctg taccggaacc gttaaaatcg
       61 cggctttgct aacatagcag gcgcgcaggt gcaacatgac ggcagcggct ggtgatgcct
      121 tgtcgcatcg ccagcggcga agattttcga cgctcattct tgacgattct ttcgacgcta
      181 ttcttccgac tcgattcggc cgccgtcttg atcggaccac caggctcgtt ggtcatcgac
      241 gaagggaatc gttccggcct caaatgcatg ggtcccatga tccgtcgcaa ggaacggttg
      301 aaagcctcga gtcattccat cttcgcattt acttgccgac acctcgtcaa agcagacgat
      361 gactttattc gacagtatat ctaagtaaca attagttaat aaactgttaa tcctaactat
      421 tacggaatat ttaaggatta gtggtctcgg taacaagatg tttcagtggg aggacaaaac
      481 catctactgc caaggtttgg cctcattagg tcaccaaacg gcaatcacgc aaagcggagc
      541 gggggttcca cttgccgcat aaatggcaat actatctcaa gggacgatat tatgctgaaa
      601 cttaagaatc tggcgcgggg aaatgtaacc ccgtcgcgta cggcgctagc tgttttcgcc
      661 gcaactctcc tgttcggtgg cagcgtcacc gaagcctcgg ccagatcgca caggcatcac
      721 caccgccatc atcatcacca ccatcaccac gccagcagca cgcccagcgc tgctgtcggc
      781 ggttctgtcg gcggttcctg gcttaacgcc aatgccgcga tcgccccttc gtccggacgt
      841 agcttctcgg gcatggcctc ctattacggc aatgaatccg gcagccggac cgcctccggc
      901 cagcgcatga acgccaacgc gatgaccgcg gcccaccgca gcctgccgtt cggcaccaag
      961 ctccgggtga cccatggcgg acgcagcgtc gtcgtcacca tcaacgaccg cggcccgttc
     1021 atccgtggcc gtgtgctcga cctgtccacc ggtgctgccc gcgtgatcgg cctgaccggc
     1081 gccggcgtcg gccgggtggt tgccgaggtc gtctcctgac ctctttgggc gtttgaacgc
     1141 tcctccggtt ccatcaagtt cggtatgtgt tgcgtagagt aaggcttgcg gaacttgctg
     1201 cacgctcatc gcccggcatt ccccgatgtg caatcgcaca tctgaggacg cgccgtttgg
     1261 cgcgggcccg gaatccatac gccctgcagg ggttatggat tccggactcg ctcgctttgc
     1321 tcgcgatccg gaatgacgat cgttttctta ttcagcgcag tgatcgaaat tgaagctaca
     1381 agaaactctg cggatcgacg tcgatctcga gcttgagatt gcctttggtc ttggggccgg
     1441 ccgccagcca ttcgcgcaaa tattccgaca gatctacgtt gcgcagcgat ttcaccaata
     1501 gccggaaacg atagcggccc ttgatcaccg ccagcggcgc ttcggcaggc cccaatacct
     1561 tgatccgctc atcgatcggc gcggtcgccg ccagcctgcg ggcaaagctc tccgccgtgg
     1621 gccgatcgcc cgcggaaatg atcaggctgg ccagccggcc gaacggcgga tagccggcgc
     1681 gctcgcgggc ctcgatctca ctggcataaa acgcctcgcg atcgcacgcc accagcgcct
     1741 tcatcacggg gtgttcgggc tgatgggtct gcagatagcc gaccccgcgg ccctgatcgc
     1801 gcccggcgcg cccgatcacc tggttgagca gttgaaaggt ccgctcggcg gcgcgtgggt
     1861 cgccattgct taagcccaaa tcagcatcga ccacaccgac caggttgagc cgcggaaaat
     1921 tgtggccctt cgctaccagc tgggtgccga tcatgatgtc gacccgcccc tccgcgatct
     1981 cattcagttc ggtgcgcatg gtctcgatcg aggtgatcag atcgctcgac agcaccatgg
     2041 tgcgcgcatc cggaaacagc tgggccgctt cctcctgcag gcgctcgacc ccggggccga
     2101 ccgcgaccag cgattgttcc gcagcacagt gcggacagac atgcggacgc ggcatcgaga
     2161 aaccacagtg atggcagacc aggcgctggc gaaagcggtg atcgaccagc caggcgtcac
     2221 agatcgtgca ggcgaaacga tgcccgcagg tccggcacaa tgtcagcggc gcgtagccgc
     2281 ggcgattgag aaacaagagc gcctgctcgc gccgctcgat cgcgatctgg acgtgctcgg
     2341 cgagccgcgg cgagatgaag cggccgcgca ccggcgcttc gcgccgcaga tcgatcgcct
     2401 cgatatgggg catgtgctga ccgccgaacc gggccggcag caccacgcgc tgataacggc
     2461 ccttgcgcgc attgacttcg gtttcgaccg aaggtgtcgc cgacgccagc acgatcggta
     2521 tcttcgcgat atgcgcgcgc accaccgcca tgtcgcgggc atggtaatgc gcgccctcgt
     2581 cctgcttgta ggcttggtcg tgctcctcat cgacgatgat caatcccaga ttggcatagg
     2641 gcagaaacag cgcagagcgc gcgcccacga ccaccggcgc ctcgcctgcc gcgatcgcgg
     2701 cccagttgcg ttgcctggtg cgtggggtca gttcggaatg ccactccagc ggccgcacgc
     2761 cgaaccgccg cgcaaagcga tcgaggaact gtccggtcag cgcgatctcc ggcatcagga
     2821 tcaacgactg cttgccgcgg cggatgactt cggcaatggc ctcgaaatag acttcggtct
     2881 tgcccgagcc ggtcacgcca tccagcagag cgacatggaa actgccgttg gcagccagcg
     2941 cgcgcatggc gtccaccgcg gtgcgctgct ggcgggaaaa gtcaggttcg gcgaacgacg
     3001 gatcgggcgc gggcggcgct tgcgcgggcg gcatcggctc ggttgcgagc gtgccttcat
     3061 cgacgaggcc atcgatcacc ccggtgctga cgccggcttc cctggctgcg tcggatttgc
     3121 cgtgcagcag accatccgac agcacctcga tcagccggcg ccgcgccggc gtcatgcgct
     3181 tgggcgtggc gccgatcaga cgcacgccca tccgcacccg ttcgggcccg agatgttcgc
     3241 ccatccgcaa gcacatccgc agcaccatgc cgcgcggcga cagcgtgtag ttggcgaccc
     3301 aatcgaccag gctgcgcagt tcctctttca gaggcggcac gtcgagcttt tcgccgacgt
     3361 ccttgaggcg attgtgcagc cgcggatcgg gattggcgtt ttccgcccag accacggcga
     3421 ccacctcgcg cggccccagc ggcacgcaca ccacatcgcc cggcttcagc tccacgccgc
     3481 gcggcacccg ataggaataa gcctgattaa gcgcgaccgg caccagcaca tcgacgacgc
     3541 gcgccgacga tgcggatgag gggctggcgc ggatggcgtg gtccattgca gaatcagact
     3601 cgaggccgga aggccgatgc taacttaggg ccccgcgccg gtcggaacca gtgtccagct
     3661 gcggccgctt tggcaagtta gcgaacggta aacgaaagaa gtgcgatata gtcatcagaa
     3721 tcatcaggtc caaaagacag ccttatggcc aagccagttc ccgctgttca gccgatcgag
     3781 cttgactgcg ccgccgacga catcgcgctt cagatgacgc gctggctgtc
//
LOCUS       DATNOI010000325        11551 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_50257,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000325 DATNOI010000000
VERSION     DATNOI010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11551
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50257"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..437)
                     /locus_tag="VN968_12880"
     CDS             complement(237..437)
                     /locus_tag="VN968_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30168.1"
                     /translation="MRAFIVATIVFGLTFGISILVLFRYGLSDNQTLPPSTAAENTLK
                     VGTLIALVIAASHWMLLPLPGW"
     gene            complement(672..747)
                     /locus_tag="VN968_12885"
     tRNA            complement(672..747)
                     /locus_tag="VN968_12885"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(712..714),aa:Lys,seq:ttt)
     gene            complement(897..3584)
                     /locus_tag="VN968_12890"
     CDS             complement(897..3584)
                     /locus_tag="VN968_12890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXL30169.1"
                     /translation="MRAVVAVLLLVTTVHAALWGVFRDKQEAPDFKGILPSVSYAPFE
                     GTSHPDVDNIPNAEKIRADLKKLASLTRAIRLYSSTGGVELVPPIAAEFGLKVTVGAW
                     IDKNVDRNEREIDAAINLAKRNSNVIGVVVGNETVYRGEQKVDDLIGLVKRVKKSVNV
                     PVTTGEIWNIWRDNPELASSVDFIAAHILPYWENFTDKQAVDQAVYIYGMLRDKFPGK
                     RIVIAEFGWPSAGYNLKNAVPGPFEQAAVLRNFVTRAESIGMEYNIVEAIDQPWKFFE
                     GGVGPYWGILNAAREPKFAWTGPIVNENYWKLALLALLVGVLMSLPILRLTEATVMQS
                     LMLSAAANGIGAWTATVFAYWTGHYFVFGSAFALTLGLILLVPLVLIAMARIEEIAAI
                     AFGRNPLRLLAKGPSAEPAVAGSEAFPAAFSKVSIHVPAYFEPPEMLKQTLDAVARLD
                     YPNFECVVIINNTPDPEFWQPIQDHCRALGERFKFINAEKVEGFKAGALRIAMERTAV
                     DAEVIGIIDADYVVQPDWLKDLVPVFADPRVGLVQAPQDHRDGNRSLMHYIMNGEYAG
                     FFDIGMVQRNEANAIIVHGTMCLIRRAAMDMAGGWNGDTICEDTDLGLAIIEHGWLTH
                     YTNHRYGHGLLPDTYEAFKKQRHRWAYGGFQIVKKHWRRFLPGASRLSPDQRREFALG
                     WLNWLGAESLGVLVAILNLIWVPIVAFADIAIPDKILTLPIIASFVVSLLHFVVLYRL
                     RVPVKWGQMLGAMIAAMSVQWTVSRAVANGLITEHLAFARTSKGGFSLMSVEFQAFWE
                     AVIGILLLVGAAVLVVTNNQKEVREIYVFAAVLVLESLPFLSAVAIAILENSRVNAFS
                     FWRHAGVRTLELIGLRPVAMPTVVSSSQPVASEIHREAS"
     gene            complement(3763..4332)
                     /locus_tag="VN968_12895"
     CDS             complement(3763..4332)
                     /locus_tag="VN968_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1-3, beta-1-6-glucan biosynthesis protein"
                     /protein_id="HXL30170.1"
                     /translation="MGRRVIQLHDAVLRLLAVPGLALLIGIGSAIAQSGDLRAQEQSG
                     KPPAANSADAAKDNQRKTDQFVEASQAINGPAGNPECVWLGRRMVRLMWNDDLDTAFR
                     HLDLYDRFGCPGGHIQATFRCLTRFGAEIDSKVPESLDRRVHACWINPNAQPQAAAVA
                     QPAPPSTAGSAAAAPVPTAPPTPAAAPAK"
     gene            4569..6194
                     /locus_tag="VN968_12900"
     CDS             4569..6194
                     /locus_tag="VN968_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-(1-6) glucans synthase"
                     /protein_id="HXL30171.1"
                     /translation="MEPISLRTPLALLLISLGAIAAVWWWLATPINLARAPIDPNAKL
                     LCVSYAPFRDTQTPLMLTTHIAPEQIAQDLAQLAKITDCVRTYSIENGLDQVPALAAK
                     VGLKVIQGIWLGSNRLKNLAQISTVVGLTKEYPSVITAVVVGNEVLLRGEMTTSDLAA
                     HIRSVKSQVAVPVTYADVWEYWLRNREVYEAVDFVTIHILPYWEDFPIRARHAASHVD
                     AIRKRMAVAFPGKEILVGETGWPSQGRMREGALPSRTNQARVVSEILLLAKQENFRVN
                     LIEAYDQPWKRELEGTVGGYWGLIDSVRRAVKYPPNEAISNFPFWKLQMGCGMALSFF
                     VFGAAGLTLRRRPWTPRIASWIAVGISATTAGILLGVAADKMFYESYGFGGWLRWGSL
                     LAAAIASPLLCANAMMSGRPLPTFLELLGPRDERARSALTMILGAVLIGTTLIGAETA
                     LGFVFDPRYRDFPFAALTMAVVPFATLMLLNRPPEGSRPITEAVFAGLLAGAALYTGF
                     NEGSVNWQSLWTCAMYFLFAVTLWRARVAQIPK"
     gene            complement(6154..6534)
                     /locus_tag="VN968_12905"
     CDS             complement(6154..6534)
                     /locus_tag="VN968_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30172.1"
                     /translation="MKKSPFRLTPYQVQWLLVVGFVTVGYALYLRYMAIEFSTVALAC
                     DAGLQTMLCKTRILVTSLFRNSVFGIAALVIATLHVIRPSIVLLTGGLIAAGFGIVLY
                     NIGLSGLAIGLLILGFARPAPATA"
     gene            complement(6825..9320)
                     /locus_tag="VN968_12910"
     CDS             complement(6825..9320)
                     /locus_tag="VN968_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020052032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HXL30173.1"
                     /translation="MNSTPTIRALVLSASILGLTAGSTLAQQTTGTPGSPSATTTIDG
                     KYLPPPPAPFGGTINLDAKDSKSYWPPTVVPPKGAPNVLLIMTDDQGYGMTSAFGGVI
                     PTPSMDRIANAGLRYTQFHSTALCSPTRAALITGRNHHSVGFGVITEMSTGYPGYDSI
                     IGPESATVGTILRENGYATSWFGKNHNTPSYQISSAGPFDQWPSGMGFDRFYGFIGGE
                     TDQWTPFLFRDQTQVYPWIGHPGYNLITDMADEAINYLKGLNNAAPDKPFFLYYVPGG
                     THAPHQPTPEWIKKISDMHLFDQGWEKLRETIFANQKRLGVIPANTQLTPWPDGQPEF
                     GGAKLQKWETLSSDEKKLFIRQADVFAAYTAYTDHEIGRVIQEVEAEGKLDNTLIIYI
                     SGDNGNSAEGSTLGTPVDMAAIQGVNIPVPDQLKFYDVWGSAETTPHMAVGWTWAFDT
                     PFKWTKQVASHFGGTRQGMAISWPSHIKDVGGIRTQFHHMIDIVPTILEATGIVAPVM
                     VNGVAQKPIEGVSMAYTFDKANANAPSTRSTQYFEMVGNRAIYHDGWLAATTPPLPPW
                     DLGLGKFPNVVNGYTWELYNLAEDYSENNDLAAKMPDRLRDMKELFLVEATKYNVFPL
                     DNSFTARAATPRPSATAGRTVFTYSGESSGLPYSSAPDITAKSYSITAEVEIPQGGAE
                     GMINTLGGRAGGHGLYLLKGKPVFVYNFFGLDRFRWEGPAALTPGKHTIVFDFKYDGP
                     GFAKGGTGVLSVDGKEVVSKTIPHTMPFLETIDETFDVGVDTRTGVEDKDYQPPFRFT
                     GKVDKLTVRLVPLNAAEEKLLQQKAQETKNKAQ"
     gene            complement(9752..10951)
                     /locus_tag="VN968_12915"
     CDS             complement(9752..10951)
                     /locus_tag="VN968_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30174.1"
                     /translation="MAKDSDHLAGSFDTENTGGFLTGFLAEEDNFDRRTLWRLGSWAA
                     ASVGAVIVAVMSNHSSIGLHRDQVAGTDLARQSQQIQSVAKESQNEARRLASVIETLN
                     GDRDRLYSRVTVLEQGLESVTGAIARQNPAAASPQAPATASAATEPQSAPQNPVAAPV
                     VSPVAAAAPATPAEKPAVDTAAARNSGPAPVPSVAQGTPNSPATPTATPLMASKSMLG
                     PPDAAALIKPETAANAVTASPMPEVVAPAASADDAERDQPWAASSKVAVQRTEFGVDV
                     GGANSLGGLRALWRGLKSNTALAALRPIIVVKESTTGLGMQLRLVAGPLADAAAAAKI
                     CAALLESQRPCETTVFDGQRLAMKAEEPPASVKPAPRKRSIGKRAVVEEPPQKPETTL
                     SSLFTRR"
     gene            11130..>11551
                     /locus_tag="VN968_12920"
     CDS             11130..>11551
                     /locus_tag="VN968_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HXL30175.1"
                     /translation="MTGRTSLTIVLAAGEGTRMQSAMPKVLHRVAGQSLLAHVLEAAP
                     KGAGAALAVVIGPDHGAVAEEVKRLRADATTFVQRERLGTAHAVLAARDAIARGADDL
                     LVVFGDTPLISAATLARMRAPLKNGAALAVLGFQAADPT"
BASE COUNT         2188 a         3491 c         3633 g         2239 t
ORIGIN      
        1 tcggcagcgg tctcgcggac gcagaacatg atgtgggcgg aggaattgga ggaccgtggg
       61 ctctggagca accgaaccag cgcggctcct cgacagccgg ctgctccgcc agcggcggct
      121 ggaaacgttt ctaaatttcc cccgcggcgg ccttgaggtt tgtggcgcgg tctgtcagcg
      181 gcccttccgc tcgcgctttt ccacacgtct tgtagatgaa catgagcgag tcggaatcac
      241 catccgggca gcggcagcag catccaatgc gatgcggcaa taactaatgc gatcagcgtt
      301 ccgaccttga gcgtgttttc ggctgctgtg cttggaggga gtgtttgatt atcgctcaag
      361 ccatacctga acagcacgag aatacttatt ccgaatgtca gaccgaacac gatggtagcg
      421 acaatgaacg cccgcatgga aggacttgcc tttagcggcc gtattttatc ggcatccccg
      481 agattgaacg ccgacttgcg ttgatcacga gccccatcac gatcattatg attacaaaca
      541 tttagcggct cacttctttt ccttcagccc gatctcgacc acgcggcggc cattttgcac
      601 cctcgtacct atggcgtacc taagtgagtg cgaactaatt tcgtaaattg atctaagcca
      661 ttgatattac ttggtgagcg cggagggact cgaaccctcg accccatgat taaaagtcac
      721 gtgctctacc acctgagcta cgcgctcact tgccgcgctg tgtagggggc ggcttggtgc
      781 gggtcaatag cgggacagcc gtaaacgggc cgttgatacc gcgcggattc gtctgccggc
      841 cccgcctgtt agcggcgatt cttcacctcc gcgaagccgc ccaaaagctg tcgcgattaa
      901 ctggcttcgc gatgaatctc ggaggccacc ggctgggagc tgctgaccac ggtcggcatc
      961 gccaccgggc gcagcccgat cagctccaag gtccgcacgc cggcatggcg ccagaacgaa
     1021 aacgcgttga cccgcgagtt ttccaggatg gcgatcgcca ccgccgacag gaacggcagg
     1081 ctttccagca ccaggacggc tgcgaacacg tagatctcgc ggacttcctt ctggttgttg
     1141 gtgaccacca gcacggccgc gccgaccagc agcaggatcc cgatcacggc ttcccagaac
     1201 gcctggaatt cgaccgacat cagcgaaaat ccgcccttgg aggtgcgcgc gaaggcgaga
     1261 tgctcggtga tcagtccgtt cgccaccgcg cgcgacacgg tccattgcac cgacatcgcg
     1321 gcgatcatgg cgcccagcat ttgcccccac ttcacgggaa cgcgcagccg gtacagcacc
     1381 acgaaatgca gcagcgagac cacaaacgat gcgatgatcg gcagcgtcag gatcttgtcg
     1441 ggaatggcga tgtcggcgaa ggccacgatc ggcacccaga tcagattgag gatcgcgacc
     1501 agcacgccca ggctttcggc gccgagccag ttcagccagc ccagcgcaaa ttcgcggcgc
     1561 tggtccggcg acaatcggct cgcgccggga agaaaacgcc gccagtgctt cttgacgatc
     1621 tggaagccgc cataggccca gcgatggcgc tgcttcttga aggcctcata ggtgtccggc
     1681 agcaggccat ggccgtagcg gtggttggtg tagtgggtga gccagccgtg ctcgatgatc
     1741 gccaggccga gatcggtgtc ctcgcagatg gtgtcgccgt tccagccgcc ggccatgtcc
     1801 atcgccgcgc gccggatcag gcacatggtg ccgtggacga tgatggcgtt ggcctcgttg
     1861 cgctggacca tgccgatgtc gaagaacccg gcatattcgc cgttcatgat gtagtgcatc
     1921 agcgaacggt tgccgtcgcg atggtcctgc ggcgcctgca cgaggccaac gcggggatcg
     1981 gcgaacaccg gcaccagatc cttcagccag tccggctgca cgacatagtc ggcgtcgatg
     2041 atgccgatga cctcggcatc cacggcggtg cgttccatgg cgatgcgcaa ggcgccggcc
     2101 ttgaagcctt cgaccttctc ggcgttgatg aatttgaaac gttccccgag cgcgcggcag
     2161 tgatcctgga tcggctgcca gaactccgga tcgggagtgt tgttgatgat caccacgcat
     2221 tcgaaattcg gatagtccag ccgggccacc gcatcgagcg tctgcttgag catctcgggc
     2281 ggctcgaaat aggccggcac atggatcgag acttttgaaa acgccgctgg aaacgcctcg
     2341 ctgcctgcaa cggccggctc ggccgatggc ccctttgcaa gcagccggag cgggttgcgg
     2401 ccgaaggcta tggcggcgat ctcctcgatc cgcgccatcg cgatcagcac cagcggaacc
     2461 agcaggatca gtcccagcgt caacgcgaag gccgagccga acacgaagta gtggccggtc
     2521 cagtacgcga acacggtagc cgtccacgcc ccgatgccgt tggcggccgc cgacagcatc
     2581 agcgactgca tcacggtggc ctcggtgagc cgcaggatcg gcagcgacat cagcacgccg
     2641 accagcaacg cgagcaaagc gagtttccag tagttctcgt tgacgatcgg cccggtccag
     2701 gcaaatttcg gctcgcgggc cgcattgaga atgccccaat aaggtccgac gccgccttca
     2761 aagaacttcc agggctgatc gatcgcctcg acgatgttgt attccatgcc gatggattcg
     2821 gcgcgggtga caaagttgcg caacacggcc gcttgctcga acgggccagg aacggcattt
     2881 ttcagattat agccggcact cggccagccg aactctgcga tcacgattcg cttgccggga
     2941 aatttgtccc gcagcatccc gtagatatag accgcctgat ccaccgcctg cttgtcggtg
     3001 aagttttccc aatagggcag aatgtgcgcg gcgatgaagt cgaccgagga ggccagctcc
     3061 ggattgtcac gccagatgtt ccagatttca cccgtcgtca ccgggacgtt gaccgacttc
     3121 ttgacccttt tgacgagccc gatgaggtca tcgacctttt gctcgccgcg gtagaccgtc
     3181 tcgttgccga cgacgacgcc gatgacgttg ctgttgcgct tggcaagatt gatggcggcg
     3241 tcgatctcgc gctcgttgcg gtcgacgttc ttgtcgatcc aggcgccgac ggtgaccttc
     3301 aacccgaatt ccgcggcgat cggcggcacc agttcgacgc cgccggttga ggagtaaagc
     3361 cggatcgccc gcgtcagcga cgccagtttt ttcaggtcgg cgcggatttt ttcggcgttg
     3421 ggaatgttgt cgacgtcggg atgcgaggtt ccctcgaacg gcgcatagga cacgctcggc
     3481 aggataccct tgaagtccgg ggcctcctgc ttgtcccgga acacccccca aagggcagcg
     3541 tgaacggtgg tgaccaagag cagaacggcg acgacggcgc gcatcgcaac aaaaccatac
     3601 ggggtcaaga tggcagggaa gcggacccgt ccccaaggcc cgaccaagtc agcggctaat
     3661 cgagcatatc tctgccacgc catttaacaa ctgttatgac gccatggcgt tttgagggcg
     3721 caaatatccg aaccgcgaaa atttctgatt gttcggttag ggttatttgg cgggcgcggc
     3781 cgcaggcgtc ggcggggccg tcgggacggg tgctgcagcc gccgaaccgg ccgtcgaagg
     3841 cggggcaggt tgggcgaccg cggcggcctg tggctgggcg ttgggattga tccagcaggc
     3901 atgaacgcgg cgatcaagcg actccggcac cttggagtcg atttccgcgc caaaacgggt
     3961 caggcaccga aatgtcgcct ggatgtggcc accgggacaa ccgaagcggt cgtagagatc
     4021 gagatgccga aaggcggtat cgagatcgtc gttccacatc aggcgcacca tgcggcgacc
     4081 aagccagacg cattccggat ttccggccgg cccgttgatg gcctgcgacg cctcgacgaa
     4141 ctggtcggtc ttgcgctgat tatccttggc ggcatcagcc gaattggccg ccggcggctt
     4201 gcctgactgc tcctgggcgc gaaggtcgcc gctttgagcg atggcgctgc cgataccgat
     4261 cagcagggcg agcccgggga cggcgagaag gcgcaaaacc gcatcgtgaa gctgaatcac
     4321 ccgtcgaccc ataaagtccc cgaattgttg cctgcctgcg gccagcactg acgcgccggc
     4381 ttgatgccgt tcaaatgggt ctccaatgcg gcggagatgc ccctgaaatc ccatgacgga
     4441 catttcggat tttgtccagc aaagtcacaa ctttatcgtg ctgtggtaaa gccgtaacgg
     4501 gggaaggccc gcatcctgct cttggcagat ggctggtgac cggctaatcg acggtccccg
     4561 cccggaggat ggaaccgatt tctcttcgta cgccactggc gcttctcctg atatccctgg
     4621 gtgcgatcgc tgccgtctgg tggtggctgg ccacgcctat caatttggcg cgcgcgccga
     4681 tcgatcccaa cgccaagctg ctgtgcgtct cctatgcgcc gttccgcgac acccagacgc
     4741 cgctcatgct gaccacccat atcgcaccgg aacagatcgc ccaggatctg gcgcagctcg
     4801 ccaaaattac cgattgcgtg cgcacctatt cgatcgaaaa cggcctcgat caagttccag
     4861 cgctggccgc caaagtggga ttgaaagtca tccaggggat ctggctcggc agcaaccgcc
     4921 tgaagaacct ggcgcagatt tcgaccgtgg ttggcctgac caaggaatac cccagcgtca
     4981 tcacggcggt ggtcgtcggc aacgaggtgc tgttgcgcgg agagatgacc acgtccgatc
     5041 tcgccgctca catccggtcg gtcaagtcgc aggtcgcggt tcccgtcacc tatgccgatg
     5101 tgtgggaata ttggcttcgc aatcgggaag tttatgaagc ggtcgatttt gtcacgattc
     5161 atatcctgcc ttactgggag gacttcccga tccgcgccag acatgccgcc tcccatgttg
     5221 acgccattcg caagcggatg gccgtggcgt tccccggcaa ggaaatcctg gtcggcgaga
     5281 ccggctggcc gagccaggga cggatgcgcg aaggggcgct gccgtcgcgt accaatcagg
     5341 cgcgagtggt ctcggaaatc ttgctgttgg cgaagcagga gaactttcgc gtcaatctga
     5401 tcgaggctta cgaccagccg tggaaacgcg agctcgaagg cacggtcggc ggatattggg
     5461 gcctgatcga ttcagtgcgg cgtgcggtga aatatccgcc caatgaggcc atcagcaact
     5521 ttccgttctg gaagctgcag atggggtgtg gaatggcgtt gagctttttc gtattcggcg
     5581 cggccgggct gaccctgcgc cggcggccct ggacgccgcg catcgcatcc tggatcgcgg
     5641 tcggcatttc cgcgaccacc gccggaattc tgttgggcgt cgccgccgac aagatgttct
     5701 atgaaagcta cggtttcggt ggctggctcc ggtggggctc gctgctggcg gccgcgatcg
     5761 cttcgccatt gctgtgcgcc aacgccatga tgtcggggcg cccgctgccg acatttctgg
     5821 aattgctggg tccccgcgac gagcgagccc gatcggcgct gacgatgatc ttgggagccg
     5881 tgctgatcgg gaccacgttg atcggcgcgg agaccgcgct cggctttgtg ttcgatccgc
     5941 gctaccgcga tttcccgttt gccgcgctga ccatggcggt ggtgccgttc gcgaccctga
     6001 tgctcctcaa ccgcccgccg gagggatcgc ggccgatcac cgaggcggta ttcgccggcc
     6061 tgctggcggg tgcggcactc tacacagggt tcaacgaagg atcggtcaac tggcaatcat
     6121 tgtggacctg cgcaatgtat tttctgttcg ccgttacgct gtggcgggcg cgggtcgcgc
     6181 aaatcccaaa ataagcaggc cgatcgccaa acccgacagg ccgatattgt agagcacgat
     6241 gccgaatccg gcggcgatca gcccgccggt gagcaagacg atcgacggtc ggatgacgtg
     6301 aagcgtcgcg atgacgagcg ccgcgatgcc gaacaccgaa ttgcgaaaca gcgacgtcac
     6361 caggatacgg gtcttgcaca gcatggtctg caggccggcg tcgcatgcca gcgcgacggt
     6421 ggagaactcg atcgccatat agcgcagata gagcgcgtag ccgacggtga cgaagccgac
     6481 gaccaacagc cattgcacct ggtacggcgt cagccgaaac ggagattttt tcactggagc
     6541 attttccggg ctgagtggaa tccggttagc cgcgagaaaa tgcgcctaac aatcatttgc
     6601 gcgtattcgg cggaatgcgc gcaaggcgaa tatggtcggg aatgcggggc gggacaaccc
     6661 ggcgcaggca gcgaacgcac caatttcgcg aaaaagcgtg agagtctgct tacaaagcat
     6721 tggaaacaga agctttaatt gatcagctag cataaccggg agcccgccgg ctctgccggg
     6781 ggatacttac aaagccaagt gccaagcaac agaggccgag cgatttactg tgccttgttt
     6841 tttgtctctt gggccttttg ctggagcagt ttttcctccg ccgcgttcag ggggaccagc
     6901 ctgaccgtta gtttatcgac cttgccggtg aaccggaacg gcggctggta gtctttgtcc
     6961 tccacgcccg tgcgggtatc cacgccgaca tcgaaggtct cgtcgatcgt ctcgaggaac
     7021 ggcatagtgt gcgggatcgt cttgctgacg acttccttgc catccaccga gaggacgccg
     7081 gtgccgccct tagcgaaacc gggaccgtca tacttgaagt caaacacgat ggtgtgcttg
     7141 ccgggcgtca gcgccgccgg gccttcccac cggaagcggt ccaagccaaa aaagttgtac
     7201 acaaagactg gtttgccctt gagcaagtag agaccatggc caccggcacg accaccgagc
     7261 gtattgatca tgccttctgc acctccctgc ggaatttcga cctctgcggt gatggagtaa
     7321 gacttggcgg taatgtcggg ggcgctactg tagggaagac cggacgattc acccgagtag
     7381 gtgaagacgg tccgaccggc ggtcgcgctc ggtcgcggcg tggcggcgcg agcggtgaag
     7441 ctgttgtcca gcgggaagac gttgtacttc gtcgcctcga ccaaaaagag ttccttcata
     7501 tcgcgcagcc tgtccggcat cttggcggcg aggtcgttgt tctccgaata atcctcggcg
     7561 agattgtaga gctcccaagt gtagccgttg acgacattgg gaaacttgcc caggcccaga
     7621 tcccagggcg gcaagggcgg cgtggtggcg gcgagccaac cgtcatggta gatggcgcgg
     7681 ttacctacca tctcgaaata ttgcgtgctg cgggtcgatg gggcgttggc gttggcttta
     7741 tcgaaggtgt aagccatgct cacgccttca atcggcttct gtgccacacc gttgaccatc
     7801 accggcgcca cgattcccgt cgcttcgagg atggtcggta cgatgtcgat catgtggtgg
     7861 aattgcgtgc gaataccgcc gacgtccttg atgtggctag gccatgagat ggccatgcct
     7921 tgacgggtgc cgccgaagtg cgacgcgacc tgcttggtcc atttgaatgg tgtatcaaag
     7981 gcccaggtcc agcccaccgc catgtgcggc gtggtctcgg ccgaacccca aacatcatag
     8041 aacttcaact ggtccgggac tggaatgttg acgccttgga tcgcggccat atcgaccggg
     8101 gtgccgaggg tggatccttc ggcgctgttg ccgttgtcgc cgctgatata gatgatgagg
     8161 gtgttgtcga gcttgccctc ggcttcgact tcctggatga ccctgccgat ttcatggtcg
     8221 gtgtaggccg tgtaggctgc aaaaacgtcc gcctgacgga tgaaaagctt cttctcgtcg
     8281 gaactgagcg tctcccattt ctgaagcttc gctccaccaa attcgggctg gccatccggc
     8341 cacggcgtaa gctgggtatt cgccgggatg acaccgagcc gcttctggtt ggcgaagatc
     8401 gtctcgcgca gcttttccca gccctgatcg aacaagtgca tgtcgctgat cttcttgatc
     8461 cactctggcg tgggctggtg cggtgcatgc gtgccgccgg gcacgtaata gaggaagaat
     8521 ggcttgtctg gcgcggcgtt attcagcccc ttcaggtagt tgatcgcctc gtcggccatg
     8581 tcggtgatga ggttgtagcc ggggtgtccg atccacggat agacttgtgt ctggtcgcgg
     8641 aagagaaacg gcgtccactg gtcggtctcg cccccgatga agccgtagaa gcgatcgaac
     8701 cccatcccgc tcggccattg gtcaaaaggc cccgccgaac ttatttgata gctgggcgtg
     8761 ttgtggttct tgccgaacca cgacgtcgcg tagccgttct cccgcaggat cgtaccgacg
     8821 gtggcactct ccggccctat gatggagtcg tatcccgggt atccagtgga catttcggtg
     8881 atcacgccga agcccaccga gtgatggttg cggcccgtga tcagcgccgc ccgggtcggc
     8941 gagcagagag cagttgagtg gaactgtgtg taacgtagtc cggcgttcgc gatgcggtcc
     9001 atggagggcg tcgggatgac accgccaaag gcgctggtca tgccgtaacc ctggtcatcg
     9061 gtcatgatca gcaagacgtt gggcgcaccc ttgggcggca ccacggtcgg cggccaatac
     9121 gattttgaat ctttggcatc cagattgatc gttccgccaa acggcgcggg cggaggcgga
     9181 agatacttgc cgtctatcgt tgttgtggcg ctaggagaac ccggcgttcc ggtggtctgc
     9241 tgcgccaagg tcgaaccagc tgtgagaccc agaatggagg ccgacaagac taatgcgcga
     9301 atggtggggg tggagttcat cgtcagactc cctcgaaatg actgttctgt agtcagaact
     9361 gtcctcgcgg aatcagcagc ctgccaaaga ccgcgacctt ggttgaccgt ggaaaattat
     9421 cggtagaccc gtcgagagac tatgcgattt cggtacaaag accccaacgt ccgaagaatc
     9481 cggtcaaaag cgtcaatcaa tcttctgtga ggcgcgtcct gcgcggcgcg agccaagttg
     9541 tcctgcggag cacgcttgag cccttcggaa attccaattc ctcaccaatc tcagcgttga
     9601 agcggctttg ttacgttgga aactacagct gtcggtcact cgcacaattg aagcaacact
     9661 gccgtggtgg tcgtcgcccg gccagtccgc gagtatagct cggcgcgcgt ggcgaaactg
     9721 tcaaggcatt ggtgtcacgc aagagccaag tctaccgcct ggtgaagagc gacgacagcg
     9781 tggtttccgg tttttggggc ggctcctcca cgacggcgcg ttttccaatg ctccgcttgc
     9841 gcggcgcagg cttgaccgaa gcgggcggct cttccgcctt catggcaaga cgctggccat
     9901 cgaacaccgt cgtctcgcat ggccgctggc tttcaagcaa ggcagcgcag attttcgcag
     9961 ccgccgcggc atcggcgagc ggtccggcaa ccagccgcag ctgcatgcca agcccggtgg
    10021 tgctttcctt caccacaatg atcgggcgta acgccgcaag cgcggtattc gacttgagac
    10081 cacgccacag cgcgcgcagg ccgccaagcg aattggcgcc gccgacatcc accccgaatt
    10141 cagttcgctg gacggcgacc ttggatgaag cagcccaagg ctgatcccgt tccgcatcat
    10201 ctgcggatgc ggccggcgca accacctccg gcatcggcga cgcggtgacg gcgttcgccg
    10261 ccgtctcggg cttgatgaga gcggccgcat cgggtggacc cagcattgac ttcgaggcca
    10321 tcagtggcgt tgctgtcggt gtcgccggcg aatttggcgt tccctgtgca acggagggga
    10381 caggcgccgg gcctgaattc cgcgcggcgg cggtgtccac agccggcttt tcggctgggg
    10441 ttgcgggcgc agccgccgcc accggcgaca ccaccggagc agcaaccgga ttttgcggtg
    10501 cggactgggg ctcagtcgcc gccgaagcgg tggcaggggc ttgcggcgat gccgctgcgg
    10561 gattttgtcg cgcaatcgcg ccggttacgg attccagtcc ctgctccaga acggtgacgc
    10621 gagaatacag ccggtcgcgg tcgccgttga gcgtctcaat gaccgacgcc agccgccgcg
    10681 cctcgttctg gctttccttg gcgacggact ggatctgctg cgactggcgc gccagatcgg
    10741 tcccggccac ctggtcgcgg tgcaatccga tcgatgaatg gttgctcatc accgccacga
    10801 tcacggcgcc gaccgacgcc gccgcccacg atccgagccg ccacagcgtg cggcggtcga
    10861 aattatcctc ctcggccaaa aaaccggtca ggaatccccc ggtgttttcg gtatcgaagc
    10921 tgccggccag gtggtcggaa tcctttgcca taggacgttc tcggccctcc tgaggcctta
    10981 gccgaatcag acggcaaaca ttaacaggaa tcgcgctgcg atccggaatc cggggcgttt
    11041 tcccgcgcga cgaacaggtt tccatcggga gcgaaaacgc tttatgacga ccacggcgca
    11101 accttcgcca ccatactata aggattcgga tgacaggcag aaccagcctc acgatcgtgc
    11161 tcgcggccgg tgagggcacg cgcatgcagt ccgcgatgcc aaaggtcctg catcgcgtcg
    11221 ccggccagtc gctgctggcg catgtgcttg aagccgcccc aaagggagcc ggcgccgcgc
    11281 tcgccgtcgt catcggaccg gaccacgggg ctgtcgcgga ggaggtcaaa cggctgcgcg
    11341 ccgatgcgac gaccttcgtt cagcgcgagc gtctcggcac cgcgcacgcg gtgctcgcgg
    11401 cgcgcgacgc cattgcccgc ggcgccgacg acctgctggt cgtgttcggc gatacgccgc
    11461 tgatttcggc cgcgacattg gcgcgcatgc gtgcaccgtt gaaaaacggg gcagcactgg
    11521 ccgtgctcgg ctttcaagcc gccgatccca c
//
LOCUS       DATNOI010000326         5513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_4527,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000326 DATNOI010000000
VERSION     DATNOI010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4527"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VN968_12925"
     CDS             <1..978
                     /locus_tag="VN968_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXL30176.1"
                     /translation="IKLARAGLREPEKPIGSYLFSGPTGVGKTEVAKQLAASLGVELI
                     RFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDGVDQHPHCVVLLDEIEKAHPDLYN
                     VLLQIMDHGRLTDHNGKQVNFRNVILIMTTNAGAADLARQAFGFTRNKREGDDHEAIN
                     RQFAPEFRNRLDAIISFAHLNAEVIGMVVEKFVLQLEAQLADRDVTIELSEPAKAWLI
                     QHGYDEQMGARPMARVIQEHIKKPLADEVLFGKLKGGGHVRVVLVKDEAATETAQEKI
                     GFEYVEGPVTPKPEKLPGTRKRTVPRKPKPSGPSGGSKGPASRGPLVKV"
     gene            complement(1092..1739)
                     /locus_tag="VN968_12930"
     CDS             complement(1092..1739)
                     /locus_tag="VN968_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXL30177.1"
                     /translation="MAVGGSYTLANWYDRQGKPRTFACRTTRVSPFRMLVQVPVIGKI
                     GSPITSFFSDFGKLEGVISDTTNGAFLLELEMSRSMREKLASKLTWLEKKLKDPRVSD
                     VRKNAGIIPANPHSMLILADGTTHSCFIIDMSIAGAAVSAEVQPPVGMPLAVGACIGR
                     VVRLLRNGIAVKFVERQNRNDLNRLIGRPALLRPSARRAKTAPSSGNTGGGDVAA"
     gene            complement(1998..2933)
                     /locus_tag="VN968_12935"
     CDS             complement(1998..2933)
                     /locus_tag="VN968_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HXL30178.1"
                     /translation="MTALSEEQSGFAPDDDAPIPYMARTREYYQAIGYATPYRWAHYV
                     DAPFQPLGKPLSQSRVTLVTTAAPFDPAKGDQGPGAKYNGGAKFYQVYDGDTSKTHDL
                     RISHVAYDRVHTSADDSGTWFPLPQLRRLAREQRIGEIAPRFFGAPTNRSHRVTLETD
                     APDILARCRAGQVDAAVLVPNCPVCHQTISLVARHLEANGIATVVIGCAKDIVEHAAV
                     PRFLFSDFPLGNSAGRPHDPTSQAFTLELALRVLEMAPAAQTTVQSPLRWSADASWKR
                     DYNNVALLSADELARRRREFDAQKEIARGLRETAA"
     gene            3029..4012
                     /locus_tag="VN968_12940"
     CDS             3029..4012
                     /locus_tag="VN968_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXL30179.1"
                     /translation="MRTAIGLHAALCIAVMALLLPVPRVIAEDAAPATGGEVAKPSVE
                     ELATPKEVQKAAENGRDTDTREAMCLMIESAARANGLPLEFFARVIWQESRFQSDAVG
                     PVTRSGQRAQGIAQFMPGTASERRLLDPFDPVQALPKSAEFLSELRSQFGNLGLAAAA
                     YNAGPRRVQEWLAGTGPMPQETRNYVVAITGSTVDDWVSAAKNSKPPDRAPNTSCRQL
                     MAMLRRAPNPFVTQLEQHVTLAAAKLWGVQLAAGFDRDKALAMYARAMKRLSAVIGDQ
                     DPSLLSSVMRTRGSHLFYQVRIGADTRPAADDLCNRIRRAGGACFVLRNRV"
     gene            4121..4957
                     /locus_tag="VN968_12945"
     CDS             4121..4957
                     /locus_tag="VN968_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HXL30180.1"
                     /translation="MPLPPQDSPSWQSPLRAFAWGVRSVASTVLTLVLFATYLGIGAL
                     AHDTHFSLGWVLAGTALVWAGPAQIILISTLGSGATVVQAAIAVTVSAFRLFPMVVSV
                     LPMMRTPRTKRRHLVLVTHFIAVTLWVECFRLLPKVPRERRVAFTLGLGTGLVCVCLV
                     ANAIGYGLAANLPQLFAAAVLLLTPLTFLLSTARNCRQLSDILALILGLALFPVAATL
                     HTGVDILISGVSAGTIAYGVHRWREHDPEKWNPVFRKDHAQNERTLESDSTQLNQTRA
                     RQ"
     gene            4954..5298
                     /locus_tag="VN968_12950"
     CDS             4954..5298
                     /locus_tag="VN968_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HXL30181.1"
                     /translation="MSHFLGDWHALLVLVIAGFLPNEVWRMLGLWLGGGVDEGSELLV
                     WVRAVATAILAGVIAQILVQPPGALASVPDWLRYGAVAAGFVAFVVTRRSIFAGVVCG
                     ELVMVAGKWWLG"
     gene            5330..>5513
                     /locus_tag="VN968_12955"
     CDS             5330..>5513
                     /locus_tag="VN968_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HXL30182.1"
                     /translation="MVLASEIREGELAVKMPRASDAGLVFIGRIRTPWTSRLEVPRQG
                     RHDGPVCRLEIFEPWVP"
BASE COUNT          963 a         1655 c         1801 g         1094 t
ORIGIN      
        1 atcaagctgg cgcgagccgg cttgcgtgaa cccgagaagc cgatcggctc gtatttgttc
       61 tccggcccga ccggcgtcgg caagacggaa gtagcgaagc agcttgcggc gtcgctcggc
      121 gtcgaactga tccggttcga catgtcggaa tatatggagc ggcacacggt gtcgcgcctg
      181 atcggcgcgc cgcccggtta cgttggcttc gatcagggcg gcctgctcac cgacggtgtc
      241 gaccagcatc cgcactgcgt ggtgttgctc gacgaaatcg agaaggcgca tcccgatctg
      301 tacaacgtgc tgttgcagat catggatcac ggccggttga ccgaccacaa tggcaagcag
      361 gtcaatttcc gcaacgtgat cctgatcatg accaccaatg cgggcgccgc cgacctggcg
      421 cgccaagcgt tcgggttcac gcgcaacaag cgggaaggcg acgatcacga ggcgatcaat
      481 cgccagttcg cgcctgaatt ccgcaaccgg ctggatgcca tcatttcgtt cgcccatctc
      541 aatgccgagg tgatcggcat ggtggtcgag aagttcgtgc tgcagctcga ggcccagctc
      601 gccgaccgtg acgtcaccat cgaattgtcc gagcctgcca aggcttggct gatacagcat
      661 ggttacgacg agcagatggg cgcccggccg atggcgcgcg tgattcagga gcacatcaag
      721 aagccgctgg cggatgaagt gctgttcggc aagctcaagg gcggcggcca cgtccgcgtc
      781 gtcttggtga aggacgaggc agcaacggag acggcccagg aaaaaatcgg cttcgaatat
      841 gtcgaagggc ccgtgacgcc aaagccggaa aagctgcccg gcacacgcaa gcgtaccgtg
      901 ccgcgcaagc cgaaaccgag tggtccgagc ggcggctcaa agggcccggc ctcacgaggt
      961 ccactggtca aggtctgagc aatcgatatc gcgaattgag gagccggcgc gtcgcaagac
     1021 cgccggcttt tttgaggagg cgacgcctcg gccaggtttc tgcgccctta ttcatgcacc
     1081 cgcgacgccg ttcaggcggc cacgtctccg ccgcccgtgt tacccgagga gggcgcagtc
     1141 tttgcacgcc ttgcggaagg ccgcagcagg gcgggtcggc caatcagtcg attgagatcg
     1201 ttgcggtttt gtctttcaac gaacttgact gcgatgccat tgcgcaaaag tctgacaacg
     1261 cggccgatgc aggctccaac ggcgagcggc attccaaccg gcggctgcac ttcagcggat
     1321 accgcggcgc cggcgatcga catgtcgatg atgaagcaac tgtgggtggt cccgtcggcc
     1381 aggatcagca ttgaatgcgg atttgccgga atgatgccgg catttttcct gacgtcggag
     1441 acgcggggat ctttcagctt cttttccagc caggtgagct tgctcgcgag cttctcccgc
     1501 atcgaccggc tcatctcgag ttcgagcagg aaggcgccgt ttgtggtgtc gctgataacg
     1561 ccctcgagct tgccgaaatc gctgaaaaaa gacgtgatgg gactgccgat cttgccgatc
     1621 actggcacct ggaccagcat ccggaacggt gagacccggg tggtccggca ggcgaaggtg
     1681 cgcggctttc cctgccggtc ataccagttt gcgagagtat agctcccgcc caccgcgatg
     1741 ttgacggctc gctatttgag gaatttttcg actgacactc ggcatactca ctaaaatcgc
     1801 atgcgttttc ctaatatgat tagtttattg aatgaacggc gctacgggaa ttttggcctt
     1861 tgaggttatc aaaggcttgc gaattcgcca cacaacagac catgtcgaac gcgcgacgag
     1921 atcccgtgcg ggaactgctg cgcgtgcccc agcagcatca actcgtcctt aaccttgaag
     1981 ccgcgcgaaa gctccactta agccgcagtc tcgcgcagtc cccgggcgat ttccttttgc
     2041 gcatcgaact cgcgccgccg ccgcgccagt tcgtctgcgc ttaacagcgc gacattgtta
     2101 tagtcgcgct tccaggacgc atccgcgctc cagcgcaacg gcgattgcac ggtggtttgc
     2161 gcagccggcg ccatttccag cacccgcaat gcgagctcca gcgtgaaggc ctgggaggtt
     2221 gggtcgtgcg gtctgccagc cgaattgccg agcggaaaat cgctgaacag gaatcttggc
     2281 accgccgcgt gctcgacgat gtccttggcg cagcctatca cgacggtagc aataccgttg
     2341 gcctcgaggt ggcgagcgac caggctgatg gtctggtggc ataccgggca attcggcacc
     2401 agcaccgcgg catctacctg gccggcgcgg cagcgcgcaa ggatgtcggg cgcatcggtt
     2461 tcgagggtga cgcgatggct gcggttggtc ggtgcgccga agaagcgcgg cgcgatctcg
     2521 ccgatccgct gctcccgcgc gagccgtcgc aattgcggca agggaaacca ggtgccgctg
     2581 tcgtcggccg aggtgtgcac gcgatcatag gcgacatgag agatgcgcaa atcatgggtc
     2641 ttcgaagtgt cgccatcgta gacctgataa aactttgcgc cgccattgta tttggcgcct
     2701 gggccctgat cgcccttggc gggatcgaac ggcgctgccg tggtcacgag ggtgacccgg
     2761 gattggctga gcggctttcc caacggctgg aacggcgcat cgacatagtg cgcccagcga
     2821 tacggcgtgg cgtatccgat cgcctgataa tattcgcgcg tccgcgccat ataggggatc
     2881 ggcgcgtcgt cgtcgggcgc gaagccggac tgttcctcgg acagtgcggt catgtcttgc
     2941 gctcccacat gcgcgccatc attctcgaga atagctaacc agtcggttcc gtgtgtcaag
     3001 catggtgttt gcggaaacaa catccccaat gcgaacagct atcggtttgc atgctgcgct
     3061 ttgcattgcg gtcatggcgc tgttgttgcc ggtgccgcgg gttatcgccg aggatgcggc
     3121 ccctgcgacc ggtggcgagg ttgcgaagcc gagcgtcgaa gaactcgcta cccccaagga
     3181 ggtgcaaaag gccgcggaga acggccgtga taccgacacc cgcgaggcga tgtgcctgat
     3241 gatcgagtcg gcggcccggg caaacggcct gccgcttgag tttttcgccc gcgtgatctg
     3301 gcaggaaagt cgctttcagt cggatgccgt gggaccggtc acgcgcagcg gccagcgcgc
     3361 ccagggcatt gcgcaattca tgccgggcac cgccagtgag cggcgcctgc tggatccctt
     3421 cgatcccgtc caggcgctac cgaaatccgc ggagttcttg agcgagttgc gcagccagtt
     3481 cggcaatctc gggctcgccg ccgcagccta caatgccgga ccgcggcggg tgcaggaatg
     3541 gctcgctggc accgggccga tgccgcagga gacgcgcaac tacgtggtcg ccatcaccgg
     3601 ctctaccgtc gatgactggg tttctgctgc caagaacagc aagccgcccg accgcgcgcc
     3661 gaacaccagt tgccgccaat tgatggcaat gctcaggcgc gcaccgaatc cctttgtcac
     3721 ccagctcgaa cagcatgtga cgctggccgc cgccaagcta tggggcgtgc agcttgccgc
     3781 gggttttgat cgcgacaagg cgctcgcgat gtatgcgcgg gcgatgaagc ggctgagtgc
     3841 cgtgatcggt gatcaggacc cgagcctgct gagctcggtg atgcgtaccc ggggcagcca
     3901 cttgttctac caggtgcgga tcggcgccga cacgcggccc gctgccgacg atctctgcaa
     3961 ccgcatccgc cgcgccggcg gcgcctgctt tgtgctgcga aacagagtgt gagggtcggc
     4021 tgatcgagcg gctgcatgac agctctgaag gcgcgttcgc ttgacgctgg ccggtttcgc
     4081 cagcgttatg cgcggcacga ctcacattgt ccggttgccc gtgccgcttc ctccgcagga
     4141 ttcaccgagt tggcaaagtc ccctgcgggc gtttgcatgg ggagtgcggt cagtcgcctc
     4201 cacggttctc acgcttgtat tgttcgcgac ctatttgggc atcggcgcgc tggcgcacga
     4261 cacgcatttc agcctcggtt gggtgctggc gggtacggct ttggtctggg ctggaccggc
     4321 gcaaatcatc ctgatctcga cactgggttc cggcgccacg gtggtgcagg cggcgattgc
     4381 cgtgacggtc agcgcgtttc ggctgtttcc catggtggtt tcggtactgc cgatgatgcg
     4441 tacgccacgg acaaaacggc gtcatctggt gctggtgact catttcatcg cggtcacctt
     4501 gtgggtcgaa tgctttcggc tactgccaaa ggtgccgcgc gagcgccggg tcgccttcac
     4561 gcttggtctc ggtacaggtc tggtttgcgt ctgcctcgtt gccaatgcga tcggctacgg
     4621 cctcgcagcc aatctgccgc aactgtttgc tgccgcggtc ttgctgctga cgccgctgac
     4681 ttttctgctg tcgaccgcgc gcaactgccg gcagctttcc gacatcctgg cgctgatatt
     4741 gggattggcg ttgtttccgg tggcggccac cttgcacacc ggcgtagata tcctgatcag
     4801 cggcgtgtcg gccggcacca tcgcctatgg cgttcaccgg tggcgagagc atgatccgga
     4861 aaagtggaac ccggttttcc gaaaagatca tgctcaaaac gaaaggacgc tcgagtctga
     4921 ttcaacccag ttgaatcaga ctcgagcgcg ccaatgagcc atttcctcgg tgactggcac
     4981 gcgctgctgg tgctcgtgat cgcgggcttc ctgcctaatg aagtctggcg gatgctcggc
     5041 ttgtggctcg gcggcggtgt cgacgagggt tccgaactgc tggtctgggt gagggcggtc
     5101 gcgaccgcga tccttgccgg cgtcatcgcg caaattctcg tgcagccgcc cggcgcgctg
     5161 gcgagtgtgc cggactggct gcgctacggc gcggtggccg ccggattcgt ggcgtttgtc
     5221 gtgacgcgac ggtcgatttt tgccggcgta gtttgcggcg aactcgtgat ggttgctggc
     5281 aaatggtggc tcgggtaact acctcatgat ggtctaacgc gaggcagaga tggttctagc
     5341 gagtgaaatc cgcgaagggg agttggcggt caagatgccc agggcgagcg atgccgggct
     5401 ggtgttcatc ggccgcatcc gcacgccctg gacctcgcgt ctcgaggtgc cgcggcaggg
     5461 gcgtcatgat ggcccggtgt gccggctcga gattttcgag ccctgggtgc cgg
//
LOCUS       DATNOI010000327        13092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1739290,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000327 DATNOI010000000
VERSION     DATNOI010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13092
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739290"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VN968_12960"
     CDS             <1..764
                     /locus_tag="VN968_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HXL30183.1"
                     /translation="ARLAIYPGTPAEVLRRLGLQRSSTNEIVPLAPAIAAPPSAAPAV
                     KAPTEAELRMSSNLTMQPKDAAEITDLFFGASSSERALILQNLLETPLKASARIPTAR
                     AARAIETLEMAAFAADVENFTLELGEALILPWRLAAQVVNDAGGEPLACAARALDMPS
                     PVFQRVLLFLNPEIGSSVNHVYRLSRLYDGLHERSALIMLAAWRGATMAVARAKYRPG
                     LHDDERHRVRSAPAQTRPATQWPGAGGVVRTRSQG"
     gene            complement(893..1237)
                     /locus_tag="VN968_12965"
     CDS             complement(893..1237)
                     /locus_tag="VN968_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HXL30184.1"
                     /translation="MRLKSNIWVAAYLRRCQTQGIFGAVRRRGAEEAGAVFVKVALLD
                     GNAMLYVPAPQTVYDDSRPIERIFTPTSREPVPEASVEERLVKEIRFDPDAWIVETED
                     RAGRHFLDLAKA"
     gene            1419..2045
                     /locus_tag="VN968_12970"
     CDS             1419..2045
                     /locus_tag="VN968_12970"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL30185.1"
                     /translation="MARAAAKKIEKKVETSTTLLEAAKKVLRQNGYAGLSTRDVAAAA
                     GVPLSQIHYHFGSKQGMVLALFEYLNAQLLDRQNAMFGDASLKLSEQWDRACDFLDDD
                     IASGYVRVLQELIAASWADAAVAQVVRGAIMGWVDLIVEAARKAERELGGLGPLTPEE
                     VGAFAANAFIGAESLYLLGLEKKGSPIRQCLRRIGDLVRSAEAARSKR"
     gene            2050..4164
                     /locus_tag="VN968_12975"
     CDS             2050..4164
                     /locus_tag="VN968_12975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL30186.1"
                     /translation="MRAKLPNETGFVDRDGVKIYYEIYGDGPETMLFLPPWSIVHSRI
                     YKAQLPYFSERFRCITFDARGNGKTDRPADVAAYSLDNNVADALAIMDATNAGNAILV
                     GLSRAGMHASVLAAHHPERVKAAILVGTVASIGPPNFSYLANKHFDAKQERFEGWNKF
                     NREYWMENYPDFAEHFVRNINSEPHSTKQIEDGISWAGDTTGPVLAKTVEARTILPPF
                     DVSEAMYRKIRCPVLIIHGDSDLLQRHARAQAVADAVTGAELVTIEGGGHNPLGRIPA
                     KTNALIVDFLDRKLGIACPARRANRSTRKARRALYLSSPIGLGHGRRDIAITRELRKL
                     RPDLEVDWLAQDPVTRLLEASGERVHPLSSRLASESRHIEMESGEHDLHCFQAIRNMD
                     EVLIANFMIFQDAVDEGGYDVVIADEAWDIDHYWHEHPELKKAGLAWFTDFVGYVPMP
                     SGGEHEAFLTADYNAEMIEHIERHPGVRDRAIFVGGPEDIVPLSFGKDLPAMRDWVPR
                     HFDFAGYIIGEHPQAFGGRAELRQSLGYRPDQRVCIVTVGGSAVGAHLIKRILQSYPV
                     ARASLPELRMIVVAGPRIDPASLHAPEGVEVYAFVPDLDRHLAACDLALVQGGLTTCM
                     ELTAAGTPFLYFPLKNHFEQNFHVAHRLDRYGAGRRMEFATSTPNMIADAMVDALREP
                     AKFKPVEAGGAACAARMLADLF"
     gene            4194..5276
                     /locus_tag="VN968_12980"
     CDS             4194..5276
                     /locus_tag="VN968_12980"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019567392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXL30187.1"
                     /translation="MTSVNTATKVDEGKLNAFIGQMLNDLGGASSIAMVRMGDTLGLY
                     KELYNKGPMTSTELANSAKVDARYLREWLSHQASSNYLSYDPTSAKFAMTPEQAMVFA
                     IPESPVYLMGGFDLMAALLDNQTKVQTAFKSGGGVAWGDQAGCMFCAVARFFRPGYHN
                     QLVSAWLPALSGGVVKKLETGAKVADVGCGHGWSTVLMAKAFPKSQFIGYDFHPASIT
                     EAKTHAEIHGVSGNTRFEVGLAKDYPGKDFDLVTCFDCLHDMGDPAGAAAHICQSLKD
                     DGTWMICEPMAGDRLEQNLNPVGRLYYAASTMICVPTSLSQEVGTALGAQAGEARLRE
                     VILKGGFKSVRRATETPFNMIIEARP"
     gene            complement(5282..5941)
                     /locus_tag="VN968_12985"
     CDS             complement(5282..5941)
                     /locus_tag="VN968_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HXL30188.1"
                     /translation="MPRQAAKGDMAPRRRGAKAVEADTERGLVLRILLHSPKDMIAGV
                     LAFAAVSAIIANALFLQAGRHPSPMFGSVVAIPAAPASLLPRPRPVEAVTRAADASPS
                     EPKPAEPKAADAMANLVKSTGAPAAAPSNTARPPAAIPALPHNETTANPAAASRRVVA
                     VQRALTEYGYGQLKPTGTIGSDTQAAIQKFERERRIPVTGQVSDRLVHELSTVIGRPV
                     E"
     gene            complement(5934..7772)
                     /locus_tag="VN968_12990"
     CDS             complement(5934..7772)
                     /locus_tag="VN968_12990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HXL30189.1"
                     /translation="MTILTIIRDCLDTLVHPSAQYDALTRARHRAFMAPRLLGSLAAL
                     AAFPVYLAMRGAPTALEVAAFAWLIAPILLSWFLSRTGRYEGAHVLSSLALAGFVVTV
                     ATTTGGIESFAAIWLVVVPLEAALSASRRVVAFASALALACTALLILAGHFHLLPAPN
                     VDAVSRGIFMAFGVASATLYAAGLAFGAESLARIGVALLYVEENRYRLLARNMSDVIS
                     RHRRNGAVQFISPAVEAMLGTQVARLLGHGLFDRVHVADRPAYLTALSDAARGGEARS
                     VEFRLRRDAPRGSERGQNIPVDFIWVEMRCRPLEQASEPTASPESEVVAVMRDVTDRK
                     IQEQALDLARTAAEQADASKTRFLATMSHELRTPLNAIIGFSEMIVQEDVLMVDAARR
                     KEYAQLINDSGQHLLSVVNGILDMSKMESGNFEILPEPFAPRPALLNCCNLLALKARD
                     NAIDLVSRVPQDLPVMTGDPRAFKQIVLNLVSNAIKFTERGGTVTVSAGVEGSRLMLR
                     VTDTGVGIAADDLKRIGDPFFQAGKTYQRRHEGTGLGLSIVKSLVGLHAGEITLQSRI
                     DEGTTVTVALPLTFTPAPPPSSNIAKLTPALRSATQDKGHQVKKSA"
     gene            8217..8606
                     /locus_tag="VN968_12995"
     CDS             8217..8606
                     /locus_tag="VN968_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5330 domain-containing protein"
                     /protein_id="HXL30190.1"
                     /translation="MFFLLRMVFWLGLVLVLLPRDKTPDSEKLPQVGASEAVSAATAA
                     VSDMSQFCKRQPAACEVGGQAATVLGQRAQDGARKLYRIITDKHAPDHTGSIDGLEDT
                     EASLADAPRDTLTPNDLQAEWRGVTTN"
     gene            8693..9124
                     /locus_tag="VN968_13000"
     CDS             8693..9124
                     /locus_tag="VN968_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HXL30191.1"
                     /translation="MMTIDEIRDNFALLDDWDDRYRYVIELGRTLDPMPEAEHSAANK
                     VQGCASQVWLAKQIDRGDANQPLLNYRGDSDAHIVRGLIAILLTLYSGHAPQEILATD
                     AIAVFDEFGFREHLTPQRSNGLRAMVERIRSDARAALATAS"
     gene            complement(9194..9607)
                     /locus_tag="VN968_13005"
     CDS             complement(9194..9607)
                     /locus_tag="VN968_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HXL30192.1"
                     /translation="MTDAAGKSFIDLTASIVSAYLRNNPTPASEIPSLISQIHAALLR
                     VSAGRVETPLEPAKPAVSVKKSMTPDYLVCLEDGKRFKSLKRHLRTQYSMTPEQYRDK
                     WGLPADYPMVAPNYAVARSQLAKKMGLGQQRRRRK"
     gene            complement(9801..10658)
                     /locus_tag="VN968_13010"
     CDS             complement(9801..10658)
                     /locus_tag="VN968_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HXL30193.1"
                     /translation="MMVLLLSVGAGLLLAGLLTVGLGIQLELSFGNTLILSGTVVACT
                     GMIMLSLWAVARELKRITLRLGSAVSMETRAGTNASRSALRNQVPEDDGFSFSRDLPE
                     LDDADDAEPPPPPLSTRREETATRDRGAPPPVPIEDAPAVKPRRNLMFSSSLRKERER
                     GQGRASDPSEADPGPAPAVESGEVSPPAFDDAWPKPERPRAAEAPPQRRGGRAPPPSA
                     EANGAADRYAPTARNEDRSSVTVLKSGVVDGMAYSLYSDGSIEAQMPEGLMRFTSIDE
                     LRAHLDQRS"
     gene            10973..12622
                     /locus_tag="VN968_13015"
     CDS             10973..12622
                     /locus_tag="VN968_13015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXL30194.1"
                     /translation="MSSITTSVIDTPSRRSVERTCDDLAIFVLAAVALIASLTFRDYG
                     LGWDDYTHAEYADLLLRMYGSGFRDTGALSFANLYMYGGGFDMAAALLHKVIPLELFE
                     TRRLLGAVVGVIGLAVTWRLGRRVGGPLAGLVALLLLALCPTFYGHMFMNPKDAPFAV
                     AMVILMLGLVRLAEEYPQPSPRTILIVGLGAGLSIGSRVLGGLALVYALIGFVPLFLE
                     EIRTHSAREAAHRLLHVVYVLFPGLILGYLVMGLIWPWSIMQPGNPLQAVTYFSHFFE
                     KPWKEMFDGALVSVPDMPWSYLPTLFALQLPEVLLGLLTAGVVGTFMSLSRSDVPGRR
                     KTILLMLTLAAMLPLLIAVLKRPALYNGIRHFIFVIPPMTVLAGTAFARGMSWLKENR
                     RSWQPAAMAVFSFGLLLPLGEMIRLHPYQYTHFNHIAGTVRAADDLYMLDYWGLALKQ
                     ASDGLREELNERQEVPPRGRKWKVAVCGPQRPAQVALGPDFTIGWDSHAADFAMTLGE
                     FYCKGLTAPVLVEIKRDDVVFARVYDIRGRSISSLLSIPAP"
     gene            12745..>13092
                     /locus_tag="VN968_13020"
     CDS             12745..>13092
                     /locus_tag="VN968_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXL30195.1"
                     /translation="MSPAEARLKEVPSDMTEAEWSQRVNLAACYRLVAYYGWDDLVDT
                     HISARVPGPEHHFLINPYGLMFDEITASSLVKVDLNGNQLTKSPYSINPAGFTIHSAI
                     HEVREDAACVIHLH"
BASE COUNT         2480 a         4154 c         4021 g         2437 t
ORIGIN      
        1 gggcgcggct tgcgatttat cccggcacac cggccgaagt cctgagaagg ctcgggctgc
       61 aacgctccag caccaacgag atcgttccgc tggcgcccgc gattgccgcg ccgccatcgg
      121 cggcgccggc ggtcaaggca ccgaccgagg cggaactgcg gatgtcatcg aatttgacga
      181 tgcagcccaa ggacgccgcc gagatcaccg acctgttttt cggcgcctcc tccagcgagc
      241 gcgcgttgat tctgcaaaat ctcctggaga caccgttgaa ggcttccgcg cgaattccca
      301 ccgcgcgcgc ggcacgtgcc atcgagacgc tggagatggc cgcgttcgcc gccgatgtcg
      361 aaaacttcac gctggaactc ggcgaagccc tgatattacc gtggcggctt gccgcacagg
      421 tggtcaacga tgccggcggt gagccgttgg cttgcgcggc cagggcgctg gacatgccaa
      481 gcccggtttt tcagcgggtg ctgctgttcc tcaatcccga aatcggatcc tcggtgaacc
      541 atgtctaccg gctgtcgcgc ctgtacgacg ggttgcacga gcgatcggcc ctcatcatgc
      601 tggccgcgtg gcgcggcgcc accatggcgg tcgcccgcgc caaatatcgc cccgggctgc
      661 acgacgacga acgccaccgc gtccgctcgg cgcccgccca gacccggccg gcgacgcaat
      721 ggcccggcgc tggcggcgtt gtgcgcacgc gttcgcaggg ttagcgcgca ttccgcaaaa
      781 gcatgccctc ggacttgatc cgggagtgga taccggtttt gcttcgcaat caagtttacg
      841 cagattgcgc agacttatct gcggtagaac acgcgcaaat tatcaaagca agctaagcct
      901 tcgccagatc gagaaaatgc cgccctgccc tgtcctcagt ctcgacgatc caggcgtccg
      961 ggtcgaaccg gatttccttg accagacgct cttccacgga cgcttccggc acgggctctc
     1021 gcgatgtcgg cgtaaaaatg cgctcgatcg gccggctgtc gtcatagacc gtctgcggtg
     1081 ccggcacgta cagcatggca ttgccgtcga gcagcgccac cttgacgaat actgccccgg
     1141 cctcttccgc gccgcgccgg cgcaccgcgc cgaatattcc ctgggtctgg cagcggcgca
     1201 aataggccgc cacccatatg ttggatttta atcgcatggc gttgacgata ggccagcgac
     1261 acaattgaag ccagagcctc ctcctccgtt cggctgatgc accgaaaacc gtttcggtgc
     1321 acaaagtcgt gaccttatgt cgaaaacatt gactcggtcg gtcgaccgaa ctactaaatg
     1381 cgacacctgc gagatcatcg cagcagggag atttcacgat ggcgcgcgcg gcggccaaga
     1441 aaatcgagaa gaaggtcgag accagcacga cgctgctcga agcggccaag aaggtgctgc
     1501 gccagaacgg ctatgctggt ctgtcgaccc gcgacgtggc ggcggccgcc ggcgttccct
     1561 tgagccagat ccactatcac ttcggatcca agcaagggat ggtgctggcg ctgttcgaat
     1621 atctgaacgc gcagctgctc gaccgccaga acgccatgtt cggcgacgcc agtctcaaat
     1681 tgtcggagca atgggatcgg gcctgcgact ttctcgatga cgacattgcc tcgggctacg
     1741 tgcgcgttct gcaggaattg atcgcggcaa gctgggccga cgccgcggtt gcccaggtcg
     1801 ttcgcggtgc gatcatggga tgggtcgatc tcatcgtcga agctgcgcgc aaggcagagc
     1861 gcgagctcgg cggcctcggc ccgctgacgc cagaggaagt cggagccttc gccgccaacg
     1921 cgttcatcgg tgcggaaagt ctctatctgc tcggtcttga gaagaaaggc tcgcccattc
     1981 gccagtgcct gcggcggatc ggcgatctcg ttcgcagcgc cgaagccgcc cgatcgaaaa
     2041 ggtgatgtga tgcgcgcaaa attgcccaac gagaccggtt tcgtcgatcg agacggcgtc
     2101 aagatctact acgagatcta cggcgacggc ccggaaacga tgctgttcct gccgccctgg
     2161 tcgatcgtcc actcgcgcat ttacaaggcc caattgccgt acttcagcga gcgtttccgc
     2221 tgcatcactt tcgatgcccg cggcaacggc aagaccgacc ggccggcgga tgtggcagcc
     2281 tactcgctcg ataacaacgt ggcggacgcg ctcgcaatca tggatgcgac caacgccgga
     2341 aacgccatcc tcgttggcct ttcccgggct ggcatgcacg ccagcgtgct ggccgcgcat
     2401 catccggaac gagtcaaggc cgcgatcctg gtcggcactg tcgcgagcat cggccccccc
     2461 aatttctcct atctggcgaa caagcatttc gatgcaaaac aagagcgatt cgagggttgg
     2521 aataaattca accgcgaata ctggatggaa aactatcccg acttcgccga gcacttcgtt
     2581 cgcaacatca attccgagcc gcactcgacc aagcaaatcg aggacggcat tagttgggcg
     2641 ggcgacacca ccgggcccgt gctggccaag accgtcgaag cgcgaacgat actgccaccc
     2701 ttcgatgtca gcgaggccat gtatcgcaag atccgctgcc ccgtgctgat catccatggc
     2761 gacagcgacc tgctccaacg ccatgcgcgc gcccaggcgg tggcggatgc cgtcaccggg
     2821 gctgagctgg tgaccattga aggcggcggc cacaatcctc tcggtcgcat tcccgcaaag
     2881 accaatgccc tgattgtcga cttcctcgat cgcaagctcg gcattgcctg ccccgcaagg
     2941 cgggcgaacc ggtcgacgag gaaagcgagg cgagcgctct atctctcctc cccgatcggc
     3001 ctcgggcacg ggcgccgcga tatcgcgatc acgcgcgagc tgcgcaagtt gcgcccggac
     3061 ctggaggtcg actggctggc gcaggatccc gtgacgcgcc tcttggaggc aagcggcgag
     3121 cgcgtccatc ccctcagttc gcggctggcg agcgaatcgc ggcacatcga gatggagtcg
     3181 ggcgagcacg atctgcactg cttccaggcc atccgtaaca tggacgaagt gctgatcgcg
     3241 aacttcatga tcttccagga tgccgtcgac gaaggcggct acgatgtcgt gatcgccgac
     3301 gaggcctggg acatcgacca ttactggcac gagcacccgg agttgaagaa agcagggctc
     3361 gcctggttca ccgatttcgt cggctacgtg ccgatgccct cgggcggcga gcatgaagcc
     3421 ttcctgaccg ccgactacaa cgccgagatg atcgagcaca tcgaacggca tcccggtgtg
     3481 cgcgaccgcg ccatctttgt cggcgggccc gaggacattg tgccgttatc gttcggcaag
     3541 gacctgccgg caatgcgcga ctgggtgccg cggcatttcg attttgccgg ttacatcatc
     3601 ggcgaacatc cgcaagcctt cggcggccgg gcggagttac ggcagagcct cggctatcgg
     3661 cccgaccaac gcgtctgcat cgtcacggtc ggcggatcgg ccgtcggcgc ccatctgatc
     3721 aagcgcatcc tgcagagtta tccggtcgcg cgagccagct tacctgagct gcgcatgatc
     3781 gtggtggccg gcccccgcat cgatccagcc tcgctccatg cgcctgaggg tgtcgaggta
     3841 tatgccttcg tgccggatct cgatcgccat ctcgcggcgt gcgatctggc gctggtgcag
     3901 ggcggcctca ccacctgcat ggagctgacc gccgccggaa cgccgttcct ctacttcccg
     3961 ctcaagaacc atttcgagca gaattttcat gttgcccatc gtctcgaccg ctatggcgcc
     4021 ggaaggcgca tggagttcgc gacgtcgacg ccgaacatga tcgccgatgc gatggtggat
     4081 gcgcttcgcg agcctgccaa attcaaaccc gtggaagccg gcggggccgc ctgcgccgca
     4141 cgcatgctgg cggatctttt ctgatcgaga actcaatttc attggaggta gctatgacga
     4201 gtgtgaatac agcgacaaaa gttgatgaag gcaagctcaa cgcatttatc ggtcagatgc
     4261 ttaacgacct cggaggtgca tccagcatcg ccatggtccg aatgggcgac acgcttggac
     4321 tctacaagga gttgtacaac aagggaccca tgacgtcgac cgagctcgcc aacagcgcga
     4381 aggtcgatgc gcggtacctg cgcgaatggc tgtcgcatca ggcgagttcc aactacctgt
     4441 cctacgatcc gacctcggcg aagttcgcta tgacgcccga gcaggccatg gtgttcgcga
     4501 ttcccgagag ccccgtctac ctgatgggcg gcttcgacct gatggcggca ctgctcgaca
     4561 accagaccaa ggttcagacc gcattcaagt ccggtggcgg tgtcgcctgg ggcgatcagg
     4621 ccggttgcat gttctgcgcc gtggcccgct ttttccggcc cggctatcac aaccagcttg
     4681 tatccgcgtg gctccctgct ctgtccggcg gcgtcgtgaa gaagctcgag acaggagcaa
     4741 aggtcgcgga cgtcggttgc ggccatggct ggtcgaccgt gctgatggcc aaggctttcc
     4801 cgaaatccca gttcattggc tacgactttc atcccgcttc aataacggaa gcaaagacgc
     4861 atgccgaaat ccacggcgtc tcgggcaaca cgcgcttcga ggtaggcctg gccaaggatt
     4921 atcccggcaa ggacttcgat ctcgtgacct gcttcgactg cctgcacgac atgggcgatc
     4981 ccgcgggcgc cgctgcccac atatgccagt ccctgaagga cgacggaacc tggatgatct
     5041 gcgagccgat ggccggcgat cgtctcgaac aaaatctcaa tcccgtgggg cgcctgtact
     5101 acgccgcatc caccatgatt tgcgtgccaa cctcgctctc gcaggaggtc ggcaccgccc
     5161 tgggcgcgca ggccggcgaa gccaggttgc gcgaggtcat tctcaagggc ggtttcaaaa
     5221 gcgttcgtcg cgctaccgag acgccgttca acatgatcat cgaagcgcgg ccttagggcc
     5281 gctattcgac cggccgacca atcacggtcg agagttcgtg caccagacga tcggagacct
     5341 gtccggtcac cgggatcctg cgctcccgct cgaatttctg gatcgcggcc tgggtgtcgg
     5401 aaccgatggt tccagtcggc ttcaactggc cgtagccata ttccgtcagc gcgcgctgca
     5461 ccgcaaccac ccggcgcgag gccgcggccg gattggctgt tgtctcgttg tggggcaaag
     5521 ccggaattgc cgccggcggc cgcgccgtat tggaaggcgc cgcggccggc gcgccggtcg
     5581 acttcaccag attggccatt gcatcggcgg ctttcggctc ggccggcttc ggttccgatg
     5641 gcgacgcgtc cgcggctctt gtcacggctt caaccgggcg cggacgtggc aacagactgg
     5701 caggcgccgc cggaatcgcg acgaccgaac cgaacatcgg cgacggatga cgtcccgctt
     5761 gcagaaacag cgcgttggcg atgatcgcgc tgaccgctgc aaatgccagc acgccggcga
     5821 tcatgtcttt cgggctatga agcaggatgc gcagcacgag gccgcgctcg gtatcggctt
     5881 cgaccgcttt cgcaccgcga cggcggggcg ccatgtcgcc ctttgcagct tgtctaggca
     5941 cttttcttca cctgatgacc tttatcctgg gttgcggatc gcaacgccgg agtgagcttt
     6001 gcgatgttgc tcgacggcgg cggcgccggc gtgaacgtca gcggcagcgc gacggtcacg
     6061 gtggtacctt catcgatccg gctctgcaag gtgatctcgc cggcgtgcaa tccgaccagg
     6121 ctcttgacaa tcgaaagccc gaggccggtt ccttcgtgcc gccgctgata ggtcttgccg
     6181 gcctggaaaa acggatcacc gatccgcttg agatcatcgg ccgcgatgcc gaccccggta
     6241 tcggtgacgc gaagcatcag ccgcgatccc tcgacgccgg cggaaaccgt caccgtgccg
     6301 ccgcgctcgg tgaacttgat ggcgttggaa acgaggttca gcacgatctg cttgaacgcc
     6361 ctggggtcgc cggtcatgac cggcaaatcc tgcggcacgc ggctgacaag atcgatggca
     6421 ttgtcccgcg ccttcagcgc cagcaggttg cagcaattga gcaacgcagg ccgcggcgcg
     6481 aacggttccg gcaaaatctc gaaattgccg gattccatct tcgacatgtc gaggatgcca
     6541 ttgaccaccg acagcagatg ctggccggaa tcattgatca gctgggcgta ttccttgcgg
     6601 cgggcagcat cgaccatcaa cacgtcttcc tgaacgatca tttcggaaaa cccgatgatg
     6661 gcattgagcg gcgtgcgcag ttcgtggctc atggtggcga ggaagcgggt cttggacgcg
     6721 tccgcctgct cggcggcggt gcgcgccaga tccagcgcct gttcctggat cttccggtcg
     6781 gtgacatcgc gcatgaccgc gaccacctcg gattcaggcg aggccgtcgg ttccgatgcc
     6841 tgttcgagcg gccggcaacg catttcaacc cagatgaaat caaccgggat gttctggccg
     6901 cgctcgctgc cgcgtggcgc atctcgcctg agacgaaatt cgacactgcg cgcctcgccg
     6961 ccgcgcgccg cgtccgaaag tgcggtcaga taggccggac gatcggcgac gtggacgcgg
     7021 tcgaacagac cgtgcccaag cagccgcgcg acctgcgttc ccaacatcgc ttccaccgcg
     7081 ggcgaaatga attgcaccgc gccgttgcga cgatggcgcg aaatcacatc gctcatgttc
     7141 cgcgccagca gacgatatcg gttctcctcg acatagagca gcgcaacgcc gatgcgcgcc
     7201 agcgactcgg cgccgaaggc aagacctgcg gcatagagcg tcgccgacgc gacaccgaac
     7261 gccatgaata tgccgcgcga caccgcgtcg acgttcggcg ccggcaacag gtgaaaatgt
     7321 ccggccagga tcagcagcgc ggtgcacgcc agcgccagcg cggatgcaaa cgcgaccacg
     7381 cggcgcgatg ccgagagtgc cgcttcgagc ggaacaacga caagccagat cgcggcaaag
     7441 gattcgatgc cgccggtggt cgttgccacc gtcaccacga aaccggcgag cgccagcgac
     7501 gacagcacat gggcgccttc gtagcggccg gtacgcgaca gaaaccacga cagcagaatg
     7561 ggcgcgatca gccaggcgaa agccgcgacc tcaagcgcgg tgggtgcgcc gcgcattgca
     7621 agatagaccg ggaatgccgc caaagccgcc aggcttccga gcagccgcgg cgccatgaag
     7681 gcgcgatggc gcgcgcgcgt cagcgcatca tattgcgctg acggatgcac cagcgtatcg
     7741 aggcaatctc ggatgatggt caaaattgtc acggctctcg cgcttcggct tgatcgtcaa
     7801 aaagacgcgc cggaacgccc ccttatcgtt accacaccgt gtcagagtga aattaagcga
     7861 acgctaaggc tcacagttcc gccgccgaag aaccgcgtgt tcgcgaggtt ttcacacacc
     7921 acgcgatggt tcattcgatg cagatggtga acgccgggtt tccacaacgc gtccagtatg
     7981 gtttcgaaat ggtggatgtg cgcgggcgca tccgttcgat tacaggaagc cgtcatcaat
     8041 caaaaattta cgccgaatcg aatcaatcgg attattcgca gattttccgc gatttcacct
     8101 caatgcaaac acttgcgatt tatcggcggc tgttagtcaa ggaccaattg cgggatcaac
     8161 cgcagcggat gcgggatcaa ccgcaacgga acaaaataga cgactgggat cgcgagatgt
     8221 tttttctgct tcgcatggta ttctggctcg ggctggtgct cgtgctgttg ccgagagaca
     8281 agacccccga ctcggagaag ctgccgcagg tcggggcgtc cgaggccgtg tccgccgcga
     8341 ccgcggcggt gtcggacatg agtcagtttt gcaagcgcca gccggcggcg tgtgaagtcg
     8401 gcggccaggc cgcgaccgtg ctcggccagc gcgcccagga cggcgcgcgc aagctctatc
     8461 gcatcatcac cgacaagcat gcgcccgatc acaccggctc gatcgacggc ctcgaggaca
     8521 ccgaggcttc gctcgcggac gcgccgcgcg acacgttgac gccgaacgat ctgcaggcag
     8581 agtggcgtgg cgtgacgaca aattagccta cggaactatt tccctttcca ccgccgatat
     8641 cgctttcgat ctcccgcgat cctatatatg gctttgggac cgagcgcggg acatgatgac
     8701 gatcgacgaa atcagggata attttgcgct gctggacgac tgggacgacc gctaccggta
     8761 tgtcatcgaa ctcggccgca cgctggatcc gatgccggaa gccgagcatt ccgccgccaa
     8821 caaggtgcag ggctgcgcca gccaggtgtg gctcgcaaag cagatcgacc gcggcgatgc
     8881 caaccaaccc ttgctgaatt atcgcggcga cagcgacgcc catatcgtgc gcggactgat
     8941 cgcgattttg ctgacgctgt attccggtca cgcgccgcaa gagattctcg ccaccgacgc
     9001 catcgcggta ttcgacgagt tcgggtttcg cgaacatctg acgccgcagc gctccaacgg
     9061 cctgcgcgcc atggtcgagc ggattcgctc cgacgcgcgc gcggcgcttg ccacggcatc
     9121 ctgaaacctc actgcaccgt cgagagaatt ggcggcggag acacgcgcgg tgcctcccat
     9181 gaaactgtca aaactatttc cgccgccgcc gctgctggcc aagccccatt ttcttggcga
     9241 gctgcgaacg cgccaccgca tagttcggcg ctaccatcgg gtaatctgcc ggcaggcccc
     9301 atttgtcgcg gtattgctcc ggcgtcatgc tgtattgcgt gcgcagatgt cgctttagcg
     9361 acttgaaacg cttgccgtcc tcgagacaga ccagatagtc gggggtcatc gattttttca
     9421 cggaaaccgc gggtttggcg ggctccagag gcgtctctac ccggcctgca gagacccgca
     9481 gcagggccgc gtgaatctgg ctgatcaggg acgggatttc cgaagccggc gtcggattgt
     9541 tgcggagata ggccgacaca atactcgcag tcaggtcgat gaaactcttg ccagcggcgt
     9601 cggtcatggc ctgaaccctt cgaagatcgt aatcgcgcca cgaatttaag gaggaactca
     9661 aggagcggaa agtatcccgc gcgagccaat acgctttcgg aaccttccac tactgtcgaa
     9721 tatgagcgga ttgttgttga cgtgacaaga acaacagcgc tctatttttc agggcgggta
     9781 caccggaagt acaggaccgg tcaagaacgt tggtcgagat gggcgcgcag ttcgtcgatg
     9841 gatgtgaacc gcatcaggcc ttctggcatt tgcgcctcga tcgagccgtc cgaatacagc
     9901 gaataggcca tgccatcgac cacgcccgat ttgagaaccg tcaccgacga ccgatcctca
     9961 tttcgcgcgg tcggtgcata acgatccgca gcgccgttgg cttcggcgga cggcggaggc
    10021 gcgcggccgc cgcggcgctg cggcggggct tcggccgccc tcggacgctc cggttttggc
    10081 caggcatcgt cgaatgctgg gggcgatacc tcgcctgatt caacagccgg cgcgggacca
    10141 ggatcagcct cggagggatc gctcgctcgc ccctgaccgc gctcgcgctc tttccgtaac
    10201 gacgaggaaa acatcagatt acgccgcggc ttgacggctg gcgcgtcctc gatgggaacc
    10261 ggcgggggcg ccccgcgatc gcgcgtcgcg gtctcctccc gccgcgtgga taacggcggg
    10321 ggcggcggct ccgcatcgtc tgcatcgtcc aattccggca ggtcgcggct gaacgagaaa
    10381 ccgtcgtctt cgggaacctg attccgcaga gcgcttcggc ttgcgttggt tccagcacgt
    10441 gtttccatcg aaacggcaga gccaagccgc aatgtaatcc gcttcagctc ccgagcgact
    10501 gcccaaaggc tgagcatgat catcccggtg catgccacga ccgtacccga gagaatcagg
    10561 gtattgccga aactcagctc gagctggata ccaagaccga ccgtcaggag cccagccaac
    10621 agcaagcctg ccccgacgct caacaacaac accatcattg agtccatctc ccggctgcgc
    10681 cagaaatgct cgcaagccgt cgacgccacg atactgtcat attgcgtacc ccgccaacgg
    10741 gcaattccgt gtgcagttcc cacgaaaaac ccgatcgacg aaccatacaa gtctggcccg
    10801 aaacgccaac cgtcacgcca tcgtgttggc ccggattgct gcactgcacc ataaaatggt
    10861 gccacaattc ccaattactt ggaaagggat ctaggctata tggatgctgg gggaatgagg
    10921 cccaaagcgc caccgctggg gtcatattag ggacgccggg ttaccaatag ccatgtcatc
    10981 cattacgact tcggtcatcg acacgccttc acggcgctcg gtggagagga cttgtgacga
    11041 cctcgccatc ttcgtgctgg cggcggttgc attgatcgca agcctaacgt tccgcgatta
    11101 cggattaggc tgggacgact acacccacgc cgaatatgcc gatctgctgc tgcgcatgta
    11161 tggttccggt ttcagggata ccggagcgct gtcgttcgcc aatctctaca tgtatggcgg
    11221 cggcttcgac atggcggccg cactgctgca caaggtcatc ccgctcgaac tgtttgaaac
    11281 gcgccgcctg ctcggcgccg tggtcggcgt gatcggcctt gcggtgacgt ggcggcttgg
    11341 ccgccgtgtc ggcggcccgc ttgcagggct agtggcgctg cttctgctgg cgctgtgccc
    11401 caccttctac gggcatatgt tcatgaatcc gaaggatgcg ccgttcgccg tcgcgatggt
    11461 gatcctgatg ctgggcctgg tccgcctggc cgaggaatat ccgcagccct cgccgcggac
    11521 catcctgatc gtcggtcttg gtgccggcct gtcgatcggt tcgcgcgttc tcggcgggct
    11581 ggccctggtc tacgcgctga tcggcttcgt gccgctgttc ctggaggaga tccgcacgca
    11641 tagcgcgcgc gaagcggccc accgcttgct gcacgttgtc tatgtgctgt tccccggcct
    11701 gatcctcggt tacctggtca tggggctgat ctggccgtgg tcgatcatgc agccgggcaa
    11761 tccgctccag gccgtgacgt atttctcgca cttcttcgag aaaccgtgga aggaaatgtt
    11821 cgatggcgcg ctggtgtcgg tgccggatat gccgtggtcc tatctgccga ccctgttcgc
    11881 tctgcagctt cccgaagtgc tgcttggtct gttgaccgcc ggtgtcgtcg gcaccttcat
    11941 gtcgctgtcg cgcagcgacg taccgggccg ccgcaagacc atccttctga tgctgacatt
    12001 ggcggcgatg ctgccgctcc tgatcgcggt gctgaagcgc ccggcgctct acaacggcat
    12061 tcgccatttc atttttgtga tcccgccgat gacggtgctg gcaggcacgg cctttgcgcg
    12121 gggaatgagc tggctcaagg aaaatcgccg cagctggcag cccgccgcca tggcggtttt
    12181 ctcgttcggg ctgctgctgc cgctcggcga aatgatccgc ctgcatccct atcaatacac
    12241 ccacttcaat catatcgcgg gtacggtgcg cgccgccgac gatctctaca tgctggacta
    12301 ttggggcctc gcgctgaagc aggcgtccga tgggctgcgc gaagagctca atgaacggca
    12361 ggaagtgccg ccgcgcggcc gcaaatggaa ggtcgccgtg tgcggtccgc aacgtcccgc
    12421 gcaggtcgcg ctcggccccg actttacgat cggctgggac agtcacgccg ccgactttgc
    12481 gatgacgctc ggcgagttct actgcaaggg cctgaccgcg ccggtgctgg tggaaatcaa
    12541 gcgcgacgac gtggtgttcg cgcgcgtcta cgatatccgc ggccgcagca tttccagcct
    12601 gctgtcgatt ccggcgccct gagacgaaga taccgtcgct gcgcgttgca atggcatgac
    12661 tgccacgcga accccgcggc ttgctgcgac gcacaggcgg ggctagtctg atggcgaaaa
    12721 tcaacatttg atgtggagac cccgatgtca ccagcggaag ctcgcctgaa ggaagtaccg
    12781 tctgatatga cggaggccga atggagccag cgggtcaatc tcgccgcctg ttatcgcctg
    12841 gtcgcgtatt atggctggga cgatctggtc gatacccata tctccgcgcg ggtgccggga
    12901 ccggagcatc atttcctgat caatccctac ggcttgatgt tcgacgaaat caccgcatct
    12961 agcctggtaa aggttgacct caacggcaac cagctcacaa aaagccccta cagcatcaac
    13021 ccggccggct tcacgattca ttccgcgatc cacgaggtgc gtgaagatgc ggcttgcgtg
    13081 attcatctgc at
//
LOCUS       DATNOI010000328         6788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_180210,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000328 DATNOI010000000
VERSION     DATNOI010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180210"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="VN968_13025"
     CDS             <1..177
                     /locus_tag="VN968_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXL30196.1"
                     /translation="LAVRKYVLQKRGVIASSAQRKPGSNITATAKAEVDYLLSRVARV
                     DKRANLQPQSSAAG"
     gene            182..979
                     /locus_tag="VN968_13030"
     CDS             182..979
                     /locus_tag="VN968_13030"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXL30197.1"
                     /translation="MTEPVTPRPASTILLLRDAAGRDEIEVFMMVRHYEIDFNSGALV
                     FPGGSVDKGDQEIIARPDLYSGGGGLDASALSFRIAAIRETFEESGILLARPHGSDAL
                     IEARRAGGIESAHRAALCEGKTSFLKVLADNGLLLALDELVPYAHWITPEGMPKRFDT
                     WFFLAAAPPEQAGAHDGKESTDSIWVSPREALAGGESGRFKLPFPTTRNLIKLGRQKS
                     VKTALDESKGKPIVSVMPVMTRLNGGRQLRIPLEAGYDGEVFEVGAV"
     gene            1119..3794
                     /locus_tag="VN968_13035"
     CDS             1119..3794
                     /locus_tag="VN968_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXL30198.1"
                     /translation="MPFAAIVQRIRSFSLSIGQLTFASFMLLLAMIVMTSVASVVSIR
                     HIDATFAELQRLQSLGDLAEEIDRRMNELRLAARDFVTDPAVPSSQVWEAASALSNLL
                     KKTRLQLAPDQQEMIDGVSARLQNYRDGIERVTALIARRAELAAALPPVRQRFEAAIA
                     EVPERITARNLFRMQNQITEALLGHDPAAAEQSAQRMRALAISEPGLRTAVDDYTDAI
                     ISIAATEGEIASIDREVLGTEGRQIGLVTELLRSISASSGRVLAHDFAKTLAHDKWQS
                     IFLGIAGVLIGLAAALFVVRRTVRPLKAIARAIRSLAGGEKDASIPATDVKNEIGDIA
                     RAAEVFRRTLVDADAAREAAVHALAEQRLAEESYRKLFEGSVDGIYVTTPAGALLNAN
                     PALARIMGYDTPEQMINSVSDIAHTIYVHPAARAEYQLLMQRDGMVREFEYQVRACDG
                     VVLWLSDSATAVRNDAGEVIRYEGTVRDITDQKRAEDAVAEGRRLLQQVIDTVPAVIN
                     VKDRQLRYVLMNRYMAGIFGVEPADAIGRTTADLMSRYGAQKTDENDKRVLASGRGLG
                     FYEEEYLDSSGNLRQWLVNKQPLLDADGEIEQIVTVALDIGERKRGEQEMRKAKDAAE
                     AALRNLRETQNSLIEAEKLAALGRLVAGVAHEVNNPVGISLTVASALERKTALFAAEV
                     ARGEIRRSHLNEFLETSRDASSQLVANLNRAAELITSFKQVAADRNYSDQRTFDLGDL
                     TEQVVMSLRPGLRKHNLTLTVDCQPNLIMNSYPGPYGQVLTNLFLNSVAHAFPDGKRG
                     TIDIRARESGKDNVEIIFSDNGCGMSLDVRRRAFDPFFTTRRDQGGTGLGLHIVYSIV
                     TNRLGGRLDLDSEPGGGTRIQLILPRVAPLEQAAE"
     gene            complement(3798..4142)
                     /locus_tag="VN968_13040"
     CDS             complement(3798..4142)
                     /locus_tag="VN968_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrlR family regulatory protein"
                     /protein_id="HXL30199.1"
                     /translation="MNGLYSIHVSLQDGRTGKGSGVVIFRDGKIVGGDAYLYYTGSYT
                     TKGDTFKGEVLVQRHTSSPDANPLFGGPSPVGIGVSGTFTDTAAVMNGTALVGKASQI
                     FTATLRKLAEIE"
     gene            complement(4286..4669)
                     /locus_tag="VN968_13045"
     CDS             complement(4286..4669)
                     /locus_tag="VN968_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL30200.1"
                     /translation="MPIVTWDHVHLRSPNPEATAAWLENILGGVIIRGPGRIDVKLGG
                     ANVFIAPVAAGDGVNAPPKTPYQGLDHFGLTVKDIDAVAAEIKAKGVEFTKEPTTIRP
                     GVRICFIRGPQGISIELLERDKKYA"
     gene            4946..5602
                     /locus_tag="VN968_13050"
     CDS             4946..5602
                     /locus_tag="VN968_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30201.1"
                     /translation="MRSPSIDGMAMGTRLYLVETTGLPGLAGIISSYSAAPAKTVARY
                     FPSKLVWLSAVLIVASGAWLVLSGSGSKTETATRAPMRLATAATSEPTQSPTTDAAQD
                     KSSVSPPATPEEAIAPVETSPVDGLKISAQSWRRGGLGSKALVTFTLRNANEYAVKDI
                     EIFCSFTRRDGSHLTDRKRVIRDTVSMRSQKTFARMHVGFVNINANQAKCSLVAASPV
                     "
     gene            5769..6164
                     /locus_tag="VN968_13055"
     CDS             5769..6164
                     /locus_tag="VN968_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30202.1"
                     /translation="MLLALLFVVDAVVPKLPAAATDRNDTDTDKAVIRIHSDRKWPER
                     VVFDTSTPTTILPAQTANVEAVVLPPTPVADVSAKTSEREAFAQFVRPEPKKPEAKLQ
                     RKRKIARSRVAPPMIQVAQQPRFGFFNTW"
     gene            complement(6248..>6788)
                     /locus_tag="VN968_13060"
     CDS             complement(6248..>6788)
                     /locus_tag="VN968_13060"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXL30203.1"
                     /translation="KSGKTFTAGQVCVLLDDISARIEVTAKLHKSLALSNNGNGVNLG
                     DFLQEIGEMIGTLGSQARMGLTVDHSCHGYVDPRHALHAGLITAELLTNAAKYAHPTG
                     LAVKVNVRCETKDDGSFMVEVRDDGVGFPESFDPSTDGGLGFQLMRALANGLNAELHF
                     EHNSLGVCARLVKPKHLAS"
BASE COUNT         1295 a         2100 c         2095 g         1298 t
ORIGIN      
        1 ctggcggtgc ggaaatatgt gctgcagaag cgcggcgtga ttgcctcgag cgctcaacgc
       61 aagccggggt ccaacattac cgccacggca aaggcggaag tggactatct gctgtcacgg
      121 gtcgcgcggg tcgacaagcg cgcgaacctg caaccgcaat ccagcgcggc gggttagccg
      181 catgactgaa cccgtcacac cgcgtcctgc ctccacgatc ctgctgctcc gcgacgcagc
      241 gggcagggat gagatcgagg tcttcatgat ggtgcgccac tatgagatcg attttaactc
      301 cggcgctctg gtgtttcccg gcggcagtgt cgacaagggc gatcaggaga tcatcgcgag
      361 acccgacctc tattccggtg gcggggggct cgatgccagc gctttaagtt tccggatcgc
      421 cgcgatccgc gaaacctttg aggagagcgg tatcctgctg gcgcggccgc acgggtcgga
      481 tgccctgatc gaagcgaggc gggctggcgg gattgaatct gcccatcgcg ccgccttgtg
      541 cgaaggcaag accagctttc tgaaggtgct cgccgacaac gggttgttgc tggcgctcga
      601 tgaactcgtg ccctacgcac actggatcac gccggagggc atgccaaaac ggttcgacac
      661 ctggttcttt cttgccgcag cgccaccgga acaggccggc gctcacgacg gcaaggaatc
      721 gaccgattcg atctgggtgt cgccgcgcga agccttggcg ggcggcgaga gcggccggtt
      781 caagctgccg tttccgacca cgcgcaacct gatcaagctc ggcaggcaaa aaagcgtcaa
      841 gaccgcgctc gacgagagca aaggcaagcc gatcgtgagc gtgatgccgg tgatgacgag
      901 gctcaacggc ggccgtcagc ttcgcatccc gcttgaggcc ggctacgacg gcgaggtatt
      961 cgaagtcggc gcggtgtaac gcaccggaac acggctgatc atcgtctggg cggcagcgcc
     1021 ggcgcctggc cgccggcatt gcggggttgc cggcatgttc cccttggggc ccattccagc
     1081 gtataaaagt ggactgctct gagccggttc cgatacccat gccctttgcc gcaatcgtcc
     1141 aacggatacg ctcattctcg ctttcgatcg gtcaattgac ctttgccagt ttcatgctgc
     1201 tgctggcgat gatcgtgatg acgagcgtcg ccagcgttgt ctctatccgc cacatcgacg
     1261 cgacctttgc cgagttgcag cggcttcaga gcctgggcga cctggccgag gagatcgacc
     1321 gccgcatgaa cgaattgcgg ctcgcggcaa gggactttgt gaccgatccg gcggttccgt
     1381 ccagccaggt ctgggaggcg gcttccgcgc tgagcaatct tttgaaaaaa acgcggctgc
     1441 agcttgcccc cgatcagcag gaaatgatcg acggcgtctc cgcgcggctc cagaactacc
     1501 gcgacggcat cgagcgcgtg accgcattga ttgcgcgccg ggcggaactg gccgccgcct
     1561 tgccaccggt gcgccagcgt ttcgaagccg cgatcgccga agtgcccgag cggatcaccg
     1621 cgcgaaacct gtttcggatg cagaaccaga ttaccgaggc gttgctgggg cacgatcccg
     1681 cggctgcgga acagtccgcc cagcgcatgc gggcgcttgc aattagcgaa ccggggcttc
     1741 gcaccgccgt cgacgactac accgacgcga ttatttcgat cgcggcgacc gagggggaga
     1801 ttgccagcat cgacagggaa gttctgggca ccgaaggtag gcagatcgga ctggtgaccg
     1861 aattattgcg cagcataagc gccagcagcg gccgcgtgct ggcccacgat tttgccaaaa
     1921 cacttgctca cgacaagtgg caaagcattt tcctcggcat tgccggggtg ttgatcgggc
     1981 ttgccgcggc gctgttcgtg gtgcgccgta ccgtccgtcc actcaaggcc atcgcgagag
     2041 ctatccgctc gctcgccggt ggcgagaagg atgcctcgat tcccgccacc gatgtcaaaa
     2101 acgagatcgg tgacattgcc cgcgccgccg aagtgttccg ccgcacgctg gtcgacgccg
     2161 acgccgcgcg agaagcggcg gttcatgccc tcgccgagca gcgccttgcc gaggaaagct
     2221 atcgcaagct gttcgagggc tcggtcgatg gaatttatgt gacgacgccg gccggcgcgc
     2281 ttctcaacgc caatcccgcg ctggcacgga tcatgggata cgatacgccg gagcagatga
     2341 tcaacagcgt cagcgatatc gcgcacacca tttatgttca tccggcggcg cgcgcggaat
     2401 accagctgct gatgcagcgc gacggaatgg tacgcgaatt cgagtaccag gtgcgcgcgt
     2461 gcgacggtgt cgtgctctgg ctctcagaca gcgctaccgc cgtgcgcaac gacgccggcg
     2521 aggtcatccg ctatgaaggc acggtgcgcg acatcaccga tcagaagcgc gccgaggatg
     2581 cggtcgccga gggccggcgg ctactgcaac aggtgatcga caccgtgccc gcggtaatca
     2641 atgtcaagga cagacagctg cgttatgttt tgatgaaccg ctatatggcc gggattttcg
     2701 gtgtcgagcc cgccgacgcg atcggccgca ccaccgccga cctgatgtcg cgctacggcg
     2761 cacagaagac cgacgagaac gacaagcgcg tgttggcgtc gggcaggggt cttggattct
     2821 acgaagagga gtatctggat tcttccggca acttgcggca gtggctggtg aataagcagc
     2881 cgcttctcga cgccgacggc gagattgagc agatcgtcac cgtcgccctc gacatcggtg
     2941 agcgaaagcg cggcgaacag gagatgcgca aggccaagga tgccgccgag gcggccttgc
     3001 gcaatctgcg ggagacccag aactcgctga tcgaggcgga aaagctcgcc gcgctcgggc
     3061 ggctggtggc cggcgtcgcc cacgaagtca acaacccggt cggcatcagc ctgacggttg
     3121 cctcagcgct ggagcgcaag acggcattgt tcgccgccga ggttgcgcgc ggcgaaatca
     3181 ggcgatccca cctcaatgaa tttctcgaga ccagccgcga tgcgtcctcg cagctggttg
     3241 ccaatctcaa tcgcgcggcc gagctgatca cctcgttcaa gcaggtggcg gccgaccgga
     3301 actactcgga tcagcgaacc ttcgacctcg gcgatctgac cgagcaggtc gtgatgagcc
     3361 tgcggccggg cttgcgaaaa cacaatctga cactcaccgt cgactgccag cccaatctca
     3421 tcatgaacag ctatcccggg ccctacgggc aggttctcac caatctgttc ctcaattcgg
     3481 tggcgcatgc ctttccggac ggcaagcgag ggacgatcga tatccgagca cgggaatccg
     3541 gcaaggataa tgttgaaatc attttttccg acaatggttg cggcatgtcc ctcgacgtcc
     3601 ggcgccgcgc cttcgatccg ttcttcacga cgcgccgcga tcagggcggc accgggctcg
     3661 ggctgcacat cgtctacagt atcgtgacca accgtttggg cggccggctc gacctcgatt
     3721 ccgagccggg cggggggacg cgcatccagc tcatcctgcc gcgggtggcg ccgctggagc
     3781 aggcggcgga atagtcgcta ttcgatctcg gccagcttgc gcagggttgc ggtaaaaatc
     3841 tggctggcct tgcccaccag cgccgttcca ttcatcacgg ccgcggtatc ggtgaaggtg
     3901 ccggagacgc cgatgccgac ggggctaggc ccgccgaaca gcgggttggc gtcgggacta
     3961 gaggtatggc gctggaccag cacctcgccc ttgaaggtat cccctttcgt cgtgtagctg
     4021 ccggtgtagt aaagataggc atcgccgccg acgatcttgc catcccggaa gatgaccacg
     4081 ccgctacctt tgccggttcg cccatcctgc aggctgacgt ggatcgaata caaaccgttc
     4141 atgacgtggc ggccggttac gacctccggc acagcggagt cggttcagct gttatgccaa
     4201 agcgcggcag gtggcgtcaa cgcgacaggt ttgtcgggcg cgtggcgccc gatgcggacc
     4261 tggacaaccg gcgtgtgccg ttttcttacg catatttctt gtcgcgctcc aatagctcga
     4321 tcgagatgcc ctgcggcccg cggatgaaac agatgcggac gcccggccga atggtcgtcg
     4381 gctccttggt gaactccacg cccttggcct tgatctcggc ggccaccgcg tcgatgtctt
     4441 ttaccgtcaa cccgaaatga tcgagcccct gatagggcgt cttcggcggc gcgttgacgc
     4501 cgtcgccggc cgcaaccggc gcgatgaaca cgttggcgcc gccgagcttg acgtcgatcc
     4561 gcccggggcc acggatgatc acgccgccga ggatattttc gagccaggcc gcggtcgcct
     4621 ccgggttggg actgcgcaaa tggacatgat cccaggtgac tatcggcatg gttgtctccc
     4681 tttgccttcc atgcgccgct tgccgcaagc gcttgcaccg gccgcttcat acggttgcac
     4741 ggcgctcgat tttagcggtc gcattctggt gttgccatcc cctctatgac tgatctgatc
     4801 ctaactgggt tttgccctct aattttgttg tgttcggatg caaccatttc gggtttcgtc
     4861 gattaaccgc ctgtggcgga accgcccagt cggcagtcgt ggcatagctg tggctcgacc
     4921 ctctcggtgc ggtacagcaa gcaatatgcg ttcgccgtct attgatggca tggcaatggg
     4981 cactcggcta tatctcgttg agacgaccgg actcccgggg ctcgccggga tcatcagcag
     5041 ctattcggca gcgccagcga aaaccgtcgc tagatatttc ccatcaaagc ttgtctggct
     5101 ttcggccgtc ctgattgtcg catcgggcgc atggctggtg ctgtccgggt ctggcagcaa
     5161 gacagaaact gccacgcgcg cgcccatgcg cttggcgact gccgctacta gcgagccaac
     5221 acaatcgcct accacggacg cggcgcagga caaatcatcc gtcagtccgc cggccacacc
     5281 tgaggaggcg atcgcacccg tggagacctc gccggtcgat gggctcaaaa tttcggcgca
     5341 gtcctggcgg aggggtgggc tcggctcgaa agccctggtc acatttacgc tccgcaacgc
     5401 caacgaatac gcggttaagg atatcgaaat tttctgttcc ttcacccgca gggacggcag
     5461 ccatctgacc gaccgtaaac gtgtgattcg tgacaccgtc agcatgagga gccagaagac
     5521 ctttgcccgc atgcatgtcg gtttcgtcaa catcaacgcc aatcaggcga aatgctcgct
     5581 ggtcgcggcc agccccgtct agctttcagg acctgcccgg ggagtaacac agcggtaaca
     5641 tcgccgttat atcggtctgg cggccctagg aaccatcaga gccatcccca gttgtgttag
     5701 agattcacga gacgtgggcg gagcctactg aaatgcttct ggcacggtat tttttgtcgg
     5761 ttggcggcgt gttgctcgcg ctgctgttcg ttgtcgatgc ggttgtgccg aagctgcccg
     5821 ctgccgctac ggacaggaac gatacagaca ccgacaaagc tgtcatcagg atccattccg
     5881 atcggaaatg gccggagcgt gttgttttcg ataccagcac cccgacgacc atcctccctg
     5941 cgcaaaccgc caatgtagag gccgttgtac tgcccccgac gccggtcgcc gacgtctcgg
     6001 caaagaccag cgagcgcgag gcgtttgcgc agttcgtgcg gcctgagcca aaaaagccgg
     6061 aagcgaagct gcaacgaaaa cgcaaaattg caagaagccg cgttgcaccg ccgatgattc
     6121 aggtcgcgca acaaccgcga tttggctttt tcaacacttg gtaagttgcg ccatccccaa
     6181 gccttggcat cgccgacatt caagccacat taccgcagcc ctgtgttcga tcgcggatca
     6241 gcgctgatta cgacgccaag tgttttggtt taacaagccg ggcgcaaacg ccaagactgt
     6301 tatgttcaaa atgcagttca gcatttagcc cattcgccag cgctcgcatc agttgaaagc
     6361 ccagccctcc gtccgttgac ggatcgaaac tctcggggaa accgacgccg tcgtccctaa
     6421 cttccaccat gaatgagcca tcgtccttcg tttcgcagcg tacattgacc ttcacagcta
     6481 aacccgtcgg atgtgcatac ttggcggcgt tcgtcaggag ctcggcggtg ataagcccgg
     6541 catgaagtgc atgacgcggg tcgacgtagc catggcagga atgatcgacg gtaaggccca
     6601 tcctcgcttg cgagccgagt gtgccgatca tctcgccaat ctcttgcaaa aagtccccga
     6661 ggtttacccc gttgccgttg ttggacagcg ccagggactt gtggagtttc gccgtgacct
     6721 cgatgcgtgc gctgatatcg tcgagcaaca cgcaaacctg ccccgccgta aaggtcttgc
     6781 cgctcttg
//
LOCUS       DATNOI010000329         2527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_827214,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000329 DATNOI010000000
VERSION     DATNOI010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827214"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VN968_13065"
     CDS             <1..223
                     /locus_tag="VN968_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30204.1"
                     /translation="MVSLQAQIIAFSQDYQMVMLFTLCAIPLAVMIGSTKAALRKQSL
                     APEHAVIEWVLAVCAAVIRGLDPRIHQSP"
     gene            complement(277..873)
                     /gene="pcaG"
                     /locus_tag="VN968_13070"
     CDS             complement(277..873)
                     /gene="pcaG"
                     /locus_tag="VN968_13070"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HXL30205.1"
                     /translation="MKAQAITPSQTVGPYFAYGLTSNGKYDWNDAFTNNLVTPDASGD
                     RIRVEGRVFDGDGQPVPDCMLEIWQADAQGRFSDPQDKRALPNSAFKGFGRCGTDANG
                     GYAFDTIKPGPVPDPDGKPQAPHVLVAVFARGMLLHLYTRIYFDGEADNAADPVLALV
                     PSDRRATLIAVREPGNGNAVYRFDIHLQGDNETVFFDV"
     gene            complement(882..1583)
                     /gene="pcaH"
                     /locus_tag="VN968_13075"
     CDS             complement(882..1583)
                     /gene="pcaH"
                     /locus_tag="VN968_13075"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HXL30206.1"
                     /translation="MTLTYPIASNTAHPPRLSPAYKSTVKRSPQKPLIIMRHTLSELT
                     GPVYGHETVRENDHDLTTQHKGEPLGERIIVHGHVLDEDGRGVPNALLEVWQANACGR
                     YVHVVDQHPAPLDPNFTGAGRAQTDNDGYYRFVTVKPGAYPWGNHHNAWRPAHIHFSV
                     FGHAFISRLVTQMYFPGDPLFPFDPIFNSVTDEKARLRMVSAFDLENTQPEWALCYRF
                     NIVLRGSGATPMETK"
     gene            complement(1739..>2527)
                     /locus_tag="VN968_13080"
     CDS             complement(1739..>2527)
                     /locus_tag="VN968_13080"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXL30207.1"
                     /translation="YDTTIGWRFINPLMKAQYGVDAMPETGENVAEEFQVSRADQDAF
                     AIRSQQRAGAAIASGYFAEEITPVSVPGGKAGPLSVDKDEHPRPETTLEGLSKLKPIV
                     RNPGTVTAGNASGVNDGAAAMILASEAAVKKHGLTPRARILGLASAAVPPRIMGIGPV
                     PATRKLMERLGLKITDFDLIELNEAFASQGIACLRQLGVKDDADFVNPHGGAIALGHP
                     LGMSGARLAMTAVHGMEKRGGKRALATMCVGVGQGVAMAIEKLN"
BASE COUNT          432 a          810 c          808 g          477 t
ORIGIN      
        1 catggtatcc ctgcaggcgc agatcatcgc cttctcacag gactaccaga tggtgatgct
       61 gttcacgctg tgcgcgatcc cgctggcggt catgatcggc tccaccaagg ccgcacttcg
      121 caagcagtcg ctggcgccgg agcatgcggt gatcgagtgg gttttggcgg tttgtgctgc
      181 tgtcatacgc gggcttgacc cgcgtatcca tcaatcgcca tgagaactat tttcgcgaaa
      241 tagatggatg gccgggtcaa gcccggccat gacgcctcac acgtcgaaaa acaccgtctc
      301 gttgtcgccc tgcaggtgga tgtcgaaacg atagaccgcg ttgccgttgc ccggctcgcg
      361 aaccgcgatc agcgtagcgc ggcgatcgga aggcaccagc gccagcaccg ggtcggccgc
      421 gttatcagcc tcgccgtcga aatagatccg cgtataaaga tgcagcagca tgccgcgcgc
      481 gaagaccgcg accaggacgt gcggcgcctg cggcttgccg tcgggatcgg gcaccgggcc
      541 gggcttgatg gtgtcgaacg catacccgcc attggcatcg gtgccgcagc ggccgaaacc
      601 cttgaacgcc gaattgggca gcgcgcgctt gtcctgcggg tcgctgaagc gcccctgcgc
      661 atcggcctgc cagatctcca gcatgcaatc cggaaccggc tggccgtcgc cgtcgaacac
      721 gcggccttcg accctgatgc ggtcgcccga tgcatccggg gtcacgaggt tgttggtgaa
      781 ggcatcgttc cagtcatatt tgccgttcga cgtcagtcca taggcgaaat acgggccgac
      841 ggtctgcgat ggcgtaatcg cctgcgcttt tattcccgac actatttcgt ctccatcggc
      901 gtggcgccgc tgccgcgcag cacaatgttg aagcgatagc acagcgccca ttccggctgg
      961 gtgttttcca gatcgaatgc ggaaaccatg cgcagccgcg ccttctcatc cgtcaccgaa
     1021 ttgaagatcg gatcgaacgg aaacagcgga tcgccgggga aatacatctg cgtcaccagc
     1081 cgcgagatga aggcgtggcc gaacaccgag aaatggatgt gggccggccg ccaggcgttg
     1141 tgatggttgc cccacggata ggcgccgggc ttgacggtga cgaacctgta atagccgtcg
     1201 ttgtccgtct gcgcgcggcc cgcgccggta aaattcgggt cgagcggtgc cgggtgctga
     1261 tcgacgacgt ggacgtagcg gccgcaggca ttggcctgcc agacctcgag cagcgcattc
     1321 ggcacgccgc ggccgtcttc atccagcaca tggccatgca cgatgatgcg ctcgccgagc
     1381 ggctcgccct tgtgctgggt ggtgagatcg tggtcgttct cgcgcaccgt ctcgtggccg
     1441 tacaccggtc cggtcagttc cgacagcgta tggcgcatga tgatcagcgg cttttgcggc
     1501 gaacgcttca ccgtgctttt gtaagccggc gacaaacgcg gcggatgcgc cgtgttgctc
     1561 gcgatgggat aggtgagtgt caaaagcgcc tcctgtctgc ggcctcatgg tggggaggcg
     1621 cttcttcagc gccgtctcga accatcagat gtgcggccca tccttcgaga cgcggccaag
     1681 tggccgctcc tcccgagtga gcgcaaatgc gctcatccgg ggatgagggg ccagtgaatc
     1741 agttcagctt ctcgatcgcc atcgcgacgc cctggccgac accgacgcac atggtcgcca
     1801 gcgcccgctt gccgccacgc ttctccatgc cgtgcactgc cgtcatcgcc agccgcgcac
     1861 cgctcatgcc caggggatga ccgagtgcga tggcgccgcc atgcgggttg acgaaatcgg
     1921 catcgtcctt gacgccgagc tggcgcaggc aggctattcc ctggctggca aaagcttcat
     1981 tgagctcgat caaatcgaaa tcggttattt tcaagcccag ccgctccatc agcttgcgcg
     2041 tcgccggcac cgggccgatg cccatgatgc gcggcggcac cgcggccgag gcgaggccca
     2101 aaatccgcgc ccgcggggtg aggccgtgct tcttcaccgc ggcttcggaa gccaggatca
     2161 tcgccgccgc gccgtcattg acgccggatg cattgcccgc ggtcaccgtg ccgggattgc
     2221 gcacgatcgg cttcagtttt gacaatcctt cgagcgtggt ttccgggcgc ggatgctcat
     2281 ccttgtcgac gctgaggggt ccggccttgc cgccgggcac cgagaccggg gtgatttcct
     2341 cggcgaaata gcctgaggca atcgccgccc ctgcccgctg ctgcgagcgg atcgcgaagg
     2401 cgtcctgatc ggcgcgcgac acctgaaact cctcggcgac attctcgccg gtctcgggca
     2461 tcgcgtcgac gccgtattgc gccttcatca gcggattgat gaaacgccag ccgatggtgg
     2521 tgtcgta
//
LOCUS       DATNOI010000330        11215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1045905,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000330 DATNOI010000000
VERSION     DATNOI010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11215
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045905"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /gene="cyoE"
                     /locus_tag="VN968_13085"
     CDS             <1..537
                     /gene="cyoE"
                     /locus_tag="VN968_13085"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HXL30208.1"
                     /translation="AAALLAFTIFFYVVVYTMWLKRRTPQNIVIGGAAGALPPVIGWV
                     AVAGNVGLEPLILFLIIFLWTPPHFWALSLNLAGEYARAGVPMLPVVAGRAETKRQIL
                     LYSVLLVPISLIPWALGFAGAIYGAAAAMLGAVMIFLSWQVHRSNDKDRRPARHLFVF
                     SMLYLVLLFAVLLMNAAR"
     gene            complement(566..1192)
                     /locus_tag="VN968_13090"
     CDS             complement(566..1192)
                     /locus_tag="VN968_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL30209.1"
                     /translation="MLTHGQIWTALDRLAERSGLSPSGLAKSAGLDPTTFNKSKRITS
                     DGRERWPSTESVAKALAATSSSIDTFVQLIGDTARAVQSVPLLGFAQAGSSGHFDDNG
                     FPAGRGWDEVALPSASDEHAYALEISGDQMNPAYRDGDVIVVSPGTPIRRGDRVVVKT
                     RVGEVMIRELKRRTAKTLELQSLNPNHADRALAAGDVEWIARIVWASQ"
     gene            1398..1742
                     /locus_tag="VN968_13095"
     CDS             1398..1742
                     /locus_tag="VN968_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HXL30210.1"
                     /translation="MRRIYKICSASAWREAERQGVYRGSADDIRDGFIHFSLPSQLAE
                     TARKHFLGQTALFLIEVDADALGDALRWEPSRGDELFPHLYGELDLGAVTNVLDMHTR
                     ADGSHDIPELAP"
     gene            1739..2833
                     /locus_tag="VN968_13100"
     CDS             1739..2833
                     /locus_tag="VN968_13100"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HXL30211.1"
                     /translation="MIRAFDAFSLPLLRWLDPEDAHRLAIHGLRLLPPIRPRPDDAKL
                     AVRAFGLNFSNPIGMAAGFDKSAEVPDSLLRLGFGFVEVGTVTPKPQAGNPRPRLFRL
                     ERDEAAVNRMGFNNDGAEAVLRRLAGRARFGGIVGVNVGANKDSADRVADYVKLIEMF
                     APVASYFTVNVSSPNTPGLRNLQQASALDDLLAKVIDARERVRQNAGDSPVLLKIAPD
                     LSLAELDDVVHIARSRRVDGMIVANTTVARPSTLREQGRAREQGGLSGRPLFRLSTRM
                     VAETYVRTEGRFPLVGVGGIDSGGAALTKIRAGASLIQLYSSLIYKGLGLVDDIKNDL
                     ASTLLRTGRDSLSEIVGADAATITAEEWPI"
     gene            complement(2830..4095)
                     /locus_tag="VN968_13105"
     CDS             complement(2830..4095)
                     /locus_tag="VN968_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXL30212.1"
                     /translation="MVANLTTPLIGIVATIAIGRLGDSALLGGVAIASVIFDCLFWLF
                     AFLRMGTLAFTAQALGAGETLELRAPLVRGFIVAALIGACLIVLQKPLAAILLGAMGG
                     SVDVTGAAKVYFTIRIWSAPLALANYVVLGWLIGQARARLALAVQIAINLINVTATAL
                     LVLVANAGIAGAAIAAVVAEAAGVALGVLIARHLTDGQLSVPRAALLDRAKLTHMLAV
                     NRDIMIRTASLIAVFLFFTAQGARAGDVTLAGNAVLNNFLLISAFFLDGLANAAEQLC
                     GRAYGARDGTGFSAAVRLVILWGAGFALAVTATYALFGPWLIDVMTASEDVRLSARHY
                     LPFVVLSPLLAVVAFAYDGVYIGATWARDMRNLMVLSLLIFLAAWLALRSFGNAGLWG
                     ALLVHYAARGGLQALRYPQLLKASFDLKR"
     gene            complement(4174..4476)
                     /locus_tag="VN968_13110"
     CDS             complement(4174..4476)
                     /locus_tag="VN968_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6460 domain-containing protein"
                     /protein_id="HXL30213.1"
                     /translation="MPNDTRQLPAAHDALYRFLGGSPLAVAFRLILLSILVGVVLAAI
                     GFDPWNILNSIQLLFRRLWDLGFDAVNWLWRYFLLGAVIVLPIWLLSRMFGASRGR"
     gene            complement(4620..6509)
                     /locus_tag="VN968_13115"
     CDS             complement(4620..6509)
                     /locus_tag="VN968_13115"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXL30214.1"
                     /translation="MNRFAELLDRLAYEPGRNNKLRLLANYFREVEDPDRGYALAALT
                     GALSFKHAKPGLIRDLIAARTDPVLFALSYDYVGDLSETVALMWPKTPPLLPRSGGEG
                     SGVGDGAAFTEAAFPADAPPAPDPSPPRAMRVEGGEIGHNNPPPPTLTEVVTTLRTLG
                     KTELPKQLTRWLDELDETGRWALLKLVTGAMRIGISARLAKTAAAELGDKDPHDIELM
                     WPGLTPPYLDLFAWLEGRAEKPVNLDPAPFRPVMLAHAIEEADFANLDAADFIAEWKW
                     DGIRVQAVSGRDARGNMLARLYSRSGEDITKSFPDLLPSLRLQDSSDLEHDASRKPLH
                     TFRHHAPFAIDGELLVVRGGRVQTFNVLQQRLNRKVVSPKLMKDYPIHLRAYDLLGEG
                     DTDLRTLPFAERRARLEAFVARLDDPRVDVSPTIAFDSWEALTAARKNPASAGAGEDA
                     EAVEGVMLKRRDALYLPGRPKGQWWKWKRDPHIIDAVLMYAQRGHGKRSSYYSDYTFG
                     VWTTGEDGELLVPVGKAYFGFTDEELLQIDRFVRRNTIEKFGPVREVVHEPDQGLVLE
                     VAFEGLQRSPRHKSGVAMRFPRINRLRWDKPPREADRLETLERMLKPDVTEAIASSSE
                     SLPRP"
     gene            complement(6506..7546)
                     /locus_tag="VN968_13120"
     CDS             complement(6506..7546)
                     /locus_tag="VN968_13120"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HXL30215.1"
                     /translation="MRPQDILMPVAAGLCCKPGGFHIDPVRPVERAAITHGHSDHARS
                     GHGAVLATQETLDMMRLRYGDNFAGSTQAIAYGEKIKLGDVTIQFHPAGHVLGSAQIA
                     VSCKDICIVASGDYKDAPDPTCAPFELVPCDVFITEATFGLPVFRHGNAADEIRKLLA
                     SVALFPERAHLVGAYSLGKAQRVIALLRAAGYDAPIYLHGAMEKITRYYESRGVALGD
                     LRPVKGMKKADLAGTITLAPPSATSDIWTRRFPDPVTAFASGWMRVRARARQGGVELP
                     LVISDHADWDGLTATIDQTGAGEVWVTHGQEDALVHWCTAKGLAARPLDLVGYGEEDE
                     GDSVLPGEGTEA"
     gene            7708..8634
                     /locus_tag="VN968_13125"
     CDS             7708..8634
                     /locus_tag="VN968_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXL30216.1"
                     /translation="MPARLFLSSGDLVADRRFEFARDLQLKGDLAAAADLLSQATELA
                     PGFISAWFTLGEIREQLGERDAAIAAFRKALTADPEDRHGASLRLMLLGAEKLSAMPE
                     GYVRALFDQYASKFEKALVGDLGYRGPALLFKAVLAARQAVRKPAFFKRAIDLGCGTG
                     LAAAAFAREVDQFIGIDLSPRMIERARATGIYAQLEVTDMVQGLRDKPDASTDLVLAA
                     DAMVYLCDLATVLREVKRVLATGGLLAFTVETHDGDGIILGEGLRYAHSKGYVRGSIG
                     DAGLALSRCEDLSARNEDNVPVPGLVVVATKS"
     gene            8753..9973
                     /locus_tag="VN968_13130"
     CDS             8753..9973
                     /locus_tag="VN968_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30217.1"
                     /translation="MSNNRSRREFCATAIATLFASATSVKAAEKKYDPGASDTEIKVG
                     QTVPHSGPGSLYGVLGRVGEAYFQMLNEKGGINGRKVKFFTMDDAYSAPKCVEATRRL
                     VEQEEVLALFGSLGTAPQTAVHKYLNSKGVPQLLLNTGASKWNDPKNNKWTMAGLPLY
                     PTEARILAKHVVSVKPNAKVGILYQNDDFGRDFLGPFKQVLADAGGTAKVIMEQTYDL
                     TDPTIDSQLINLSKSGADVFYNISTGKASSQSIRKVAELGWKPLQLLSAGSTGRSILS
                     AAGLENAAGIVAIRYAKEVGVPRWENDPDVMAFEELRKKYLPNVDPDNTIAFAGYGQV
                     ATMAEVLRRCGDDLTRANVLKQATNLNGYHSPYFLDGISYSYTPDDYTPMKTLYISVF
                     NGKDWDISDRPVTE"
     gene            10083..>11215
                     /locus_tag="VN968_13135"
     CDS             10083..>11215
                     /locus_tag="VN968_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXL30218.1"
                     /translation="MPPQTLLESPTETLLPDRFRRWFAGRGWSPREHQLALLAKARDD
                     RSALLIAPTGAGKTLAGFLPTLVELSCPLPPRSGGEGSEVGGLSTRSHARTETAEAPP
                     TPDPSPPRAGRAGGGEPARSPRLNVISTGRGVRRSLGLHTLYISPLKALAVDIARNLE
                     TPVSEMGLPIKVETRTGDTPVSRRQRQRRYPPDILLTTPEQLALLLSSDDAPFLFSSL
                     KRIVLDELHALVTSKRGDLLSLGLARLWRLAPGMRSIGLSATVAEPKSLARFLVPQRG
                     GASEAADIVVAGGAAAPIVEMLDTSERLPWAGHTARHALGEIYDLIKRNKTTLVFVNT
                     RSQAEMLFQDLWRMNDDGLAIALHHGSLDVAQRRKVEDAMARG"
BASE COUNT         2057 a         3566 c         3601 g         1991 t
ORIGIN      
        1 gcggctgcgc tgcttgcttt cacaattttc ttctatgtcg tcgtctacac gatgtggctc
       61 aaacgccgaa caccgcaaaa tatcgtcatt gggggtgcgg ctggcgcact ccctccagtg
      121 atcggctggg ttgcggtcgc cggaaacgtt gggctcgaac cgctcatcct gttcctgatc
      181 atcttccttt ggacgccacc ccacttctgg gcactgtcgc tcaatcttgc cggagaatat
      241 gctcgcgccg gtgtgccgat gttgccggtc gtagccggca gggccgaaac aaagcgccag
      301 attcttctct atagcgttct tctggttccg atctcactga taccttgggc actggggttc
      361 gcgggggcca tctatggcgc ggctgcggcg atgcttggag cggtcatgat ttttctctca
      421 tggcaagtac atcgtagcaa tgataaggac aggcggcctg ctcgccacct gtttgtgttt
      481 tcgatgctct atctggtcct gctttttgct gtgctcttga tgaatgctgc acgatagagc
      541 gtaacccctc acccggttcg aaagtttact ggctcgccca cacaatccgc gcgatccact
      601 cgacatcgcc ggccgcaagc gcgcgatcgg cgtggttcgg attgagcgat tgcagctcca
      661 gcgtcttcgc ggtccggcgt ttcaattccc tgatcatcac ttcgccgact cgcgttttca
      721 ccaccacgcg gtcgccgcgc cggatcggtg tgccaggcga caccacgatg acatcgccgt
      781 cgcggtaggc gggattcatc tggtcgccgg atatttcgag cgcatacgca tgctcgtcac
      841 tggccgaagg caaggcgacc tcatcccagc ccctgccggc cgggaaacca ttgtcgtcaa
      901 aatggccgct gctgccggcc tgcgcgaaac cgagcagcgg caccgactgc acggcgcgcg
      961 cggtatcgcc gatcaactgc acgaaggtgt cgatggagct gctggtggcc gcgagcgcct
     1021 tggccaccga ttcggtggaa ggccagcgtt cgcggccgtc agaggtgatg cgcttggatt
     1081 tgttgaaggt ggtgggatcg aggccggcgc tcttggcaag cccggaagga gacagcccgg
     1141 agcgctcggc aagccggtcc agcgcggtcc agatctggcc gtgggtcagc atgcgcggtg
     1201 ttttcggctg cctggccatc ggtcgatttt gccatcctag gaattatctc ctcaatagac
     1261 cggcttttcc gagctggggc aagagccgta atgcgacgct tgaagtgcgc gggtagcgcc
     1321 gatacggtcg gcgcgtgatc cggcaaattc cgggaaaagc ccaaaagact caacaacaag
     1381 caggaactcc gtaacgcgtg cgcaggatct acaaaatctg ttccgcttca gcctggcgcg
     1441 aggccgagcg gcaaggcgtg taccgcggca gcgccgacga tatccgcgac ggcttcattc
     1501 atttttcgct cccgtcgcag ctggcggaga cggcaagaaa gcacttcctc gggcagaccg
     1561 cgctgttcct gatcgaggtc gatgccgacg cgctggggga cgcgctgcgg tgggagccgt
     1621 cgcgcggcga cgaattgttt ccgcacctct acggcgaact cgatctcggc gcagtcacca
     1681 atgtgctgga catgcacacg cgcgccgacg ggtctcacga cattccggag ctggcgccgt
     1741 gatccgcgcc ttcgatgcgt tttcgctgcc gctgttgcgc tggctcgatc cggaggacgc
     1801 gcatcgcctg gcgatccacg gcttgcggct gctgccgccg atccggccgc ggccggacga
     1861 tgcaaaattg gcggtgcggg ccttcggctt gaatttttcc aatccgatcg gcatggcggc
     1921 gggcttcgac aagagcgcgg aggtgccgga ttccttgttg cggcttggtt tcggttttgt
     1981 cgaggtcggc acggtcacgc cgaagccgca ggccggcaat ccgcggccac gcctgttccg
     2041 cctggagcgc gacgaggccg cggtcaatcg catgggtttc aacaatgacg gggcggaggc
     2101 ggtgctgcgg cggctggccg ggcgcgcgcg tttcggcggc attgtcgggg tcaatgtcgg
     2161 cgccaacaag gattccgccg accgcgtcgc cgactatgtg aaactgatcg agatgtttgc
     2221 acccgtggca agctatttca ccgtcaatgt gtcgtcgccg aacacgccgg gcttgcgcaa
     2281 cctgcagcag gcttccgcgc tcgacgacct cctggcaaag gtgatcgatg cgcgcgaacg
     2341 cgtgcggcag aacgccggcg actcgccggt gctcctcaag atcgcacctg atctgagtct
     2401 cgccgaactc gatgatgtcg ttcacatcgc ccgctcgcgc cgggtcgacg gcatgatcgt
     2461 ggccaatacc acggtggcgc ggccctcgac gctccgcgaa cagggccggg ccagggagca
     2521 ggggggactc tcgggtaggc cgctgtttcg actgtcgaca cggatggtgg ccgagactta
     2581 cgtgcgcacc gagggtaggt ttccgctggt cggcgtcggc ggcatcgatt ccggcggcgc
     2641 cgcgctgaca aaaatccgcg ccggcgccag tctgatccag ctctactcgt cgctgatcta
     2701 caagggcctt ggactggtgg acgacatcaa gaacgatctc gcctcgacgc tgctccgcac
     2761 cgggcgcgat tcgctctccg aaatcgtcgg cgccgacgcg gcgacgatca cggccgagga
     2821 atggccgatt taacgcttca aatcaaatga ggccttcagg agctgaggat agcgcaacgc
     2881 ctgcaatccg ccccgtgcgg cgtagtgcac cagcaaagcg ccccacaggc cggcattgcc
     2941 gaacgagcgc aacgccagcc acgcggcgag aaagatcagc agcgaaagca ccatcaggtt
     3001 gcgcatatcg cgcgcccagg ttgcgccgat atagacgccg tcataggcga aagcgacgac
     3061 ggcaagcaag ggagacagga ccacgaaggg taaatagtga cgcgcgctga ggcgaacatc
     3121 ttcgctcgcg gtcatgacgt cgatcagcca ggggccgaat agcgcgtaag tcgccgttac
     3181 cgcaagcgca aagcctgctc cccacaggat caccagccgg accgcagctg aaaagccggt
     3241 tccgtcgcgc gcgccataag cgcggccgca gagttgctcg gcggcgttgg cgagcccgtc
     3301 gaggaagaag gcgctgatca aaaggaaatt gttcagcacc gcgttgccgg cgagcgttac
     3361 gtcgccggcg cgggcgccct gcgcggtaaa aaacaggaac acggcgatca acgacgcggt
     3421 gcggatcatg atatcgcggt tgacggccag catgtgggtc agtttggcac ggtcgagcag
     3481 cgccgctcgc gggacggaaa gctgtccatc ggtgaggtgc cgcgcaatca aaacgccgag
     3541 cgccaccccg gccgcttccg ccacgacggc ggcaatcgcc gcgccggcga tcccggcgtt
     3601 cgctaccagc accagcagcg cggtcgccgt gacattgatg aggttgatcg cgatctggac
     3661 ggcaagcgca agtctggcgc gtgcctgtcc gatcagccag ccgagcacga cgtaattggc
     3721 cagcgccagc ggcgcagacc agatccggat ggtgaagtag accttcgctg cgccagtgac
     3781 gtccacgctg ccgcccatgg cgccaagcag gattgcggcg agcggtttct gcaggacgat
     3841 caggcaggcg ccaatcagcg cggcaacgat aaatccccgc accagcggcg cgcgcaattc
     3901 cagcgtttcg ccggcgccaa gcgcttgtgc agtgaaagcc agcgttccca tgcgcaggaa
     3961 cgcgaacagc caaaacaggc agtcgaaaat caccgacgcg atcgccacgc cgccgagtag
     4021 cgcggaatcg ccgagccgtc cgatcgcgat cgtcgcgaca ataccgatca gcggcgtggt
     4081 caggttcgca accatcgcgg gacccgcgat ggcgaagacc tgtgcggtcg taaccttcga
     4141 tggaagaact agcaaaccat ggacgcccgc gatctatcgt ccgcgcgacg cgccgaacat
     4201 tcgcgacaag agccagatcg gaaggacgat caccgcgccg agcaggaaat agcgccataa
     4261 ccagttgacg gcgtcaaagc caagatccca gagccgccga aacaacagct ggatgctgtt
     4321 gagaatattc cagggatcga aaccgatggc ggcgagcacc acgcccacga gtatcgacag
     4381 caggatcagc cggaacgcca ccgccagcgg cgagccgccg agaaagcgat agagggcgtc
     4441 atgggctgcc ggcaattgcc gggtgtcgtt gggcatcgat gatctcccgc gcggatttat
     4501 cagcgcattg tagtacggcg gagggcagat ggggaacccg cttgttggcg tcaatggccg
     4561 gcgaccggcg ctctttattt tgacgcgttt tcttcacgcg aaccggtgta caggccgcat
     4621 caagggcggg gcaggctttc gcttgacgac gctatcgcct cagttacgtc gggtttcagc
     4681 atccgctcca gcgtttccag ccggtcggcc tcgcgcggcg gcttgtccca gcgcagccgg
     4741 ttgatgcggg gaaaccgcat cgccacgccg gatttgtgcc gcggcgagcg ttgcaggccc
     4801 tcgaaagcga cttccagcac caggccctga tcgggctcgt gcaccacctc gcgcaccggg
     4861 ccgaattttt cgatggtgtt gcggcggacg aagcgatcga tctgcagcag ttcctcgtcg
     4921 gtgaagccga aataggcctt gccaaccggc accagcagct cgccgtcctc gcctgtggtc
     4981 cagacgccga acgtatagtc cgagtaatac gacgaccgct tgccgtggcc gcgctgcgca
     5041 tacatcagca cggcatcgat gatgtgcggg tcgcgcttcc atttccacca ttgtcctttc
     5101 ggacggccgg gcaaatacaa cgcgtcgcgg cgcttcagca tcacgccttc gaccgcctcg
     5161 gcgtcctcgc ccgcaccggc gctggcggga ttttttcgtg cggcggtgag ggcctcccag
     5221 ctgtcgaagg caatcgtcgg cgacacgtcg acgcgcgggt catcgagcct ggcgacaaag
     5281 gcctcaagcc gcgcgcggcg ttcggcaaac ggcagggtac gcaggtcggt gtcgccctcg
     5341 ccgagcagat cgtaggcgcg cagatggatc ggataatcct tcatcaactt cggcgagacg
     5401 accttgcggt tgaggcgttg ctgcaggacg ttgaaggttt gcacgcggcc accgcgaacc
     5461 accagcagct cgccgtcgat ggcgaatgga gcatgatgcc gaaaagtgtg aagcggtttt
     5521 cgggaagcat catgctccaa atctgaagaa tcttgcagac gcaatgacgg cagaaggtcc
     5581 ggaaagcttt tggtgatgtc ttcgccgctg cgtgaataga gccgcgccag catgttgccg
     5641 cgcgcgtcac ggccgctgac cgcctggacg cgaatgccgt cccatttcca ttccgcgatg
     5701 aaatcggcgg cgtcaagatt ggcaaagtcg gcctcctcga tcgcatgcgc cagcatcacg
     5761 ggtcggaacg gcgcgggatc gagattgacc ggtttctcgg cgcggccttc cagccaggcg
     5821 aacagatcga gatagggcgg ggtgagcccc ggccacatca attcgatgtc gtgcgggtcc
     5881 ttatccccaa gctcggccgc ggcggtcttc gccagccgcg ccgagatccc gatccgcatc
     5941 gcgccggtga cgagtttcaa cagcgcccag cggccggtct catccagctc gtcaagccag
     6001 cgcgtgagct gcttcggcaa ctcggtcttg ccaagcgtgc gcagcgtggt cacgacctcg
     6061 gtgagggtgg gagggggcgg gttgttgtgg ccaatctccc ctccctccac gcgcatcgcg
     6121 cgtggcgggg aggggtcggg ggcggggggt gcatccgcag gaaacgcggc ttcggtgaac
     6181 gcggcgccgt cccccacccc cgacccctcc ccgccgcttc gcgggaggag gggaggagtc
     6241 ttcggccaca tcagcgccac cgtctcggac aaatcgccta cgtaatcata cgacagcgcg
     6301 aacagcaccg gatcggtgcg ggcggcgatc aaatcgcgga tcagcccggg ttttgcgtgt
     6361 ttgaacgaaa gtgcgcccgt gagcgcggcg agcgcgtagc cgcgatcggg atcttcgacc
     6421 tcgcgaaaat agttcgcaag aagccgcagc ttgttgttgc ggccgggctc ataggcgagg
     6481 cgatcgagca attcggcgaa gcggttcatg cctcggtgcc ctcaccgggg agcacgctat
     6541 cgccttcatc ctcctcgccg taaccgacga ggtcgagcgg ccgcgcggcg agccccttgg
     6601 ccgtgcacca atgcaccagc gcgtcttctt ggccatgggt gacccagacc tcaccggcgc
     6661 cggtttgatc gatggtcgcg gtcagtccgt cccaatcggc atggtccgag atcaccagcg
     6721 gcagttcgac gccgccctgc cgggcccgcg cccgcactcg catccagccc gaggcgaacg
     6781 ccgtgaccgg atcgggaaac cgccgcgtcc agatatcgga ggtggccgaa ggcggcgcga
     6841 gcgtgatggt gccggcgagg tcggccttct tcatgccttt gaccgggcgc aggtcgccca
     6901 gcgccacgcc gcgactctcg taatagcgcg tgatcttttc catcgcgccg tgcagataga
     6961 tcggtgcgtc gtaaccggcg gcacgtaaca gcgcgatcac ccgctgcgcc ttgccaagcg
     7021 aataggcgcc gaccaaatgc gcccgctccg gaaacagcgc caccgaagcc agcagcttcc
     7081 tgatctcgtc ggcggcattg ccgtgacgaa acaccggcag gccaaacgtc gcctcggtga
     7141 tgaacacatc gcaagggacc agttcgaacg gcgcgcaggt ggggtcgggc gcgtccttgt
     7201 agtcgccgga ggcgacaatg cagatatcct tgcaggagac cgcgatctgc gccgagccca
     7261 acacatggcc tgcgggatga aactggatgg tgacgtcgcc gagcttaatc ttttcgccat
     7321 aggcgatggc ttgcgtgctg ccggcaaaat tgtcgccgta gcgcagccgc atcatgtcga
     7381 gcgtctcttg ggtggccagc accgcgccat ggcccgaccg ggcgtggtcg gaatggccgt
     7441 gggtgatcgc ggcgcgctcg accggccgca ccggatcgat atggaagccg ccgggcttgc
     7501 aacatagccc agcggcaacc ggcatcagga tgtcttgcgg gcgcatgtcg gttatatagg
     7561 gcctgttgcc gcgattttta cccgtcatgc gcgggcttga cctgcgcatc catcttccgg
     7621 aagaattctt cgatcggtgg attgccggat cgtctccgac caggtcgggg acaggccccc
     7681 ggcaacgaca aaggtggtgc caaaaaaatg cccgcgcgct tgttcctgtc ctccggcgat
     7741 ctggttgccg accgccggtt cgaatttgca cgcgatctgc aactgaaagg cgatctcgcc
     7801 gccgccgccg atctgctctc gcaggctacc gaactcgcgc cgggctttat ttccgcctgg
     7861 tttacgctgg gcgaaattcg cgaacaactg ggcgagcgcg atgcggcgat cgcggcattt
     7921 cgcaaagcgc tgaccgcaga tcccgaggac cggcatggcg cgagcctgcg gctgatgctg
     7981 ctcggagccg aaaagttgtc ggcgatgccg gaaggctatg tgcgcgcgct gtttgatcaa
     8041 tacgcgtcca aattcgagaa ggcactggtc ggcgatctcg gctatcgcgg cccggcgctt
     8101 ttattcaagg cggtgctggc ggcgcggcag gcggtccgta aacctgcctt cttcaagcgc
     8161 gccatcgatc tcggctgcgg tacggggtta gctgccgccg cctttgcccg cgaggtcgac
     8221 caattcatcg gcatcgatct gtcgccacgc atgatcgaac gggcgcgcgc caccggaatc
     8281 tatgcgcagc tcgaggtcac ggacatggtg cagggtttgc gcgacaagcc tgacgccagc
     8341 accgatctcg ttctcgccgc cgacgccatg gtctatctgt gcgatctcgc aaccgtcctg
     8401 cgcgaggtga agcgcgtgct ggcgaccggc ggcttgctgg cgttcaccgt ggaaacgcat
     8461 gatggcgacg gcatcattct cggcgagggg ctgcgttatg cccacagtaa gggctatgtg
     8521 cgcggctcga tcggcgatgc cggacttgcg ctgtcgcgat gtgaggatct gtcggcccgc
     8581 aacgaggaca acgtaccggt tcccggtctt gtcgtggttg cgacgaaatc ctgagtattt
     8641 tctcgcaagg catgcctcaa aagcggggcc gcatgcgccg caaagccgcg aattcgctat
     8701 tgccgcgcac gcggtaatgt agatcaatgc gcgctccagg gagaaaacga taatgagcaa
     8761 caatcggtcg aggcgtgagt tctgcgccac cgctatagcg acgctcttcg cgtccgccac
     8821 atccgtcaag gccgccgaga aaaaatacga tccgggcgcg agcgacaccg aaatcaaggt
     8881 cggccagacc gtgccgcaca gtggcccggg ctcgctttac ggcgtgctcg gccgggtcgg
     8941 cgaagcctat ttccagatgc tcaacgaaaa gggcggcatc aacggacgca aggtgaaatt
     9001 cttcaccatg gacgacgcct atagcgcgcc gaaatgcgtt gaggcgacgc ggcggctggt
     9061 ggagcaggag gaagtgcttg ccctgttcgg ttcgctcggt accgcgccgc agacggccgt
     9121 gcacaaatat ctcaattcca aaggcgtgcc gcagcttctg ctcaacaccg gcgcatctaa
     9181 atggaacgat ccgaaaaaca acaagtggac catggcgggc ttgccgctct atccgaccga
     9241 ggcacgaatt ctcgccaagc acgtggtgag cgtgaagccg aacgcgaagg ttgggatcct
     9301 ctatcagaac gacgattttg gccgcgattt cctgggtccc ttcaagcagg tgctggcgga
     9361 tgccggcggc accgccaagg tgatcatgga gcagacttac gatctgaccg atccgaccat
     9421 tgattcgcag ctaatcaatc tctcgaagtc gggcgccgac gttttttata acatctccac
     9481 cggcaaggcg tcgtcgcagt cgatccgcaa agtggcagaa ctcggctgga agcctctaca
     9541 attgctttcc gcgggatcca ccggccgctc gatcctcagt gctgccggcc ttgagaacgc
     9601 ggccgggatc gtcgccatcc gctacgccaa ggaagtcggc gtgccgcgct gggagaacga
     9661 tcccgacgtc atggcattcg aggagttgcg caagaagtac ctgcccaatg tcgatcccga
     9721 caataccatc gcattcgcag gctatggcca ggtggcgacg atggctgaag tcctgcgccg
     9781 ctgcggcgac gatctcacgc gcgccaatgt cctgaagcag gcgacgaacc tcaacgggta
     9841 tcactcacca tatttcctcg acggcatcag ctacagctac acccctgacg actacacgcc
     9901 gatgaagacg ctctatatct ccgtgttcaa cggcaaggac tgggacattt ccgacaggcc
     9961 ggtcacggaa taacgcgtct tctccctctc cccgcccttt gcggggagag gtgactcgaa
    10021 ttcggggccg gtattccctg ccgccctcga cgaagcgggt ttaaccgctt acctcttggc
    10081 gcgtgccgcc ccaaaccctt ctcgaatcgc cgaccgaaac gctgctgccg gaccgctttc
    10141 gccgctggtt cgcgggccgc ggctggtcgc cgcgcgagca tcagctggcg ctgctggcaa
    10201 aggcccggga cgatcgctcg gcactcttga ttgcgccgac cggcgccggc aagacgctgg
    10261 ccggattcct gccgacactg gtggagcttt cttgtcccct ccctccgcga agcggcgggg
    10321 aggggtcgga ggtagggggt ctctccactc gctctcatgc tcgcaccgag actgcggagg
    10381 caccccccac ccccgacccc tccccgccac gcgccgggcg cgcgggggga ggggagcccg
    10441 cgaggtcccc gcgtctcaat gtcatctcca ccgggcgcgg cgtgcggcga agcctcggtc
    10501 tccacaccct ctacatctcg ccgctgaagg cgctcgcggt cgatatcgcg cgcaatctgg
    10561 aaacgccggt gagcgagatg ggtctgccga tcaaggtcga gacccgcacc ggcgacacgc
    10621 cggtgtcgcg gcggcagcgg cagcgccgct atccgcccga cattctgctc accacgccgg
    10681 aacaactggc gctgttgctc tcatccgacg atgcgccgtt tctgttctca tcgctgaagc
    10741 gcatcgtgct cgacgagttg cacgcactgg tgacctcgaa gcgcggcgat ctgttgtcgc
    10801 tcgggttggc gcggctgtgg cggctggcgc ccgggatgcg ctcgatcggg ctgtcggcca
    10861 cggtggcgga gccgaaatcg ctggcgcggt ttctggtgcc gcagcgcggc ggcgcaagcg
    10921 aagccgccga catcgtcgtc gccggcggcg ccgcggcgcc gatcgtcgag atgctcgata
    10981 caagcgaaag attgccgtgg gccggccata ccgcgcgcca cgcgctcggc gaaatctacg
    11041 acctgatcaa gcgcaacaag accacgctgg tgttcgtcaa tacccgcagc caggccgaga
    11101 tgctgttcca ggatctgtgg cgcatgaacg atgatgggct ggcgatcgcg ctgcatcacg
    11161 gctcgctcga tgtcgcacag cgccgcaagg tcgaggacgc gatggccagg ggaaa
//
LOCUS       DATNOI010000331         3290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1995434,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000331 DATNOI010000000
VERSION     DATNOI010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1995434"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VN968_13140"
     CDS             <1..472
                     /locus_tag="VN968_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXL30219.1"
                     /translation="AQVSVPVYQGGAEYALIRQSKETLAQQRLNLEQVRDQTRANVVT
                     AWGQLVAGKAQVASAQSQVAASEIALNGVREEAKAGQRTTLDVLNAQQALVNARVALV
                     TAQHDRVVASYSVLNTIGRLSPQVLNLATTVYDPSVHYQQVRDSWMGVRTPDGR"
     gene            935..1705
                     /locus_tag="VN968_13145"
     CDS             935..1705
                     /locus_tag="VN968_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HXL30220.1"
                     /translation="MTQPAKVQEPSMEEILASIRRIIADDEARPAGAEKAPGPTAAKL
                     EKPAAVAPAVKAPVMTNIPPSAIPAAQAAAAKAPPAPAKPAPPPAAPPPAAAAPVSNS
                     QDDIDAMLAGLDEATSEAEIRPAAPEVEVFELTDEMAVPEPPQPAFHKVEPQDDLEFT
                     ETAAARAVHRRPAFEPPPFESSAPPAQQILSRSTVSAVESAFNSLANTVLSNNARTLE
                     DLVKEMLRPMLKSWLDDNLPGLVERIVKAEIERVSRGR"
     gene            2070..>3290
                     /locus_tag="VN968_13150"
     CDS             2070..>3290
                     /locus_tag="VN968_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXL30221.1"
                     /translation="MIEKNYQPADIEGRMSRIWEESGAFRAGRPERRDAKPFTIVIPP
                     PNVTGSLHMGHALNNTLQDILCRFERMRGRDVLWQPGTDHAGIATQMVVERQLMERQQ
                     PGRRDMGRAKFLERVWQWKAESGGVIVNQLKRLGASCDWSRERFTMDEGLSRAVVKVF
                     VELHREGLIYKDKRLVNWDPKLLTAISDLEVQQVEVKGSLWYLRYPLEGRTFNPDDPT
                     TFIVVATTRPETMLGDSAVAVHPDDERYADLVGKHVILPLVGRKIPILADEYSDPEKG
                     SGAVKVTPAHDFNDFEVGNRHGLPRISVLDQEGRLTLVGNEDYLRGLPEESEYFAEEL
                     NGLDRFVARKLIVARLEDFGFLERIEPNTHMVPHGDRSGVIIEPYLTDQWYVDAKTMA
                     RPAMAAVRSGATTFV"
BASE COUNT          635 a         1014 c         1075 g          566 t
ORIGIN      
        1 cgcgcaggtt tcggtcccgg tgtatcaggg cggtgccgag tatgcgctga tccgccaatc
       61 gaaggaaaca ttggcgcagc agcgcctcaa cctcgaacag gttcgcgacc agacccgtgc
      121 caacgttgtt acggcttggg ggcagttagt ggcaggcaag gcgcaggtcg cttcggcgca
      181 atcgcaggtg gccgcatccg agatcgcgct caacggcgtg cgcgaagagg ccaaggccgg
      241 acagcgaacc acgctcgacg tgctgaacgc ccagcaggcg ctggtcaacg cccgcgtcgc
      301 gctcgtcacc gcgcagcacg atcgggtggt cgcctcatat tccgtcttga ataccatcgg
      361 ccggttgtcg ccgcaggtgc tcaatctggc caccaccgtg tacgatccga gcgtgcatta
      421 tcagcaggtt cgcgacagct ggatgggcgt tcgcactccc gacggccgct gatcgccgcc
      481 gccggcgtcg aagcacggac gccgttcata gcgcgcgatc agcaagagtg gggcccggtt
      541 tcgcggccaa acttggtcgc tggtccgcag atcgcgctct gggttaatgc agtgagcgca
      601 tggtgttttc gccggaccgc ccacaccttc ggggcacgcc caagggtgtg cgttggcggc
      661 tcaagcgctg gcgcgttgct tgcaatcaat tgtggccatg acgtaccttt ctcgggggca
      721 gcagagcgat tcgcgtcgaa ttcacatcct aaggcgcgcg gcgcttcggt gtctgggcag
      781 gggcgaaaat cgggcgcgag ccatcgagcg gcgcttgatc tggggacaag taggctgctg
      841 cgatggaaat cccggcctgc ctatccggaa tcggcaaatc ctgtcttgct tggtgtaaaa
      901 cgcatacgag ggcgttgatg atgtggggtc ggagatgacg cagcctgcaa aggtccaaga
      961 gccctcgatg gaggagattc tggcgtcgat ccggcgcatc attgccgacg atgaagcaag
     1021 acctgcgggc gccgagaagg cccccggccc gacggcagcg aagctggaga agcccgccgc
     1081 agtggcgccg gcggtcaagg cgcccgtgat gactaacatt ccgccgtcgg cgattcccgc
     1141 cgcgcaagcg gctgctgcga aggcgccgcc ggcgcccgcc aagcccgcgc cacccccggc
     1201 tgcgccaccg cctgccgcgg ccgcccccgt gtccaacagc caggacgaca tcgacgcgat
     1261 gctggccggc cttgatgaag cgacgtccga ggccgagata aggccagccg cgcccgaggt
     1321 cgaggtgttc gaactcaccg acgagatggc ggtgccggag ccaccgcagc ctgcgttcca
     1381 caaggtcgaa ccgcaggacg atctcgaatt caccgaaacc gccgctgcca gggccgtgca
     1441 ccggcggccg gcgttcgaac cgccgccttt tgaaagttcg gcgccgccgg ctcagcagat
     1501 attgtcccgc tcaaccgtct ccgccgtcga atcggccttc aacagcctgg ccaatacagt
     1561 gttgagtaac aacgcgcgca cactggaaga tctggtcaag gaaatgctgc gcccgatgct
     1621 gaaatcctgg ctcgacgaca atctgcccgg gctggtggag cgcatcgtca aggcggaaat
     1681 cgagcgggtt tcgcgcgggc gctagcgcat gatccggaaa aagcctgccc cgcacttgat
     1741 gcggggtggg caccggtttt ccgaaaagat catgctcaaa caaagagata gagcgggatg
     1801 acgattcgaa gaagagtcat cccgcgctgg cgccctgttc cgctcgcgga gggcaattgc
     1861 cggccctcgt tcagccactg catccggctt aatcctgcgt tccacgcgcc tgtcgtgttg
     1921 acttggcgcg ttccggcggc tttctacagc atgatccgga aaagtggata ccggttttcc
     1981 gaaaagatca tgctcaaaca aagagatgag atcatgatcc gatccaatcg gttcatgatc
     2041 tagcgttcca tagccatgat tgcattgcca tgatcgagaa aaactaccag cccgccgata
     2101 tcgaaggccg catgtcccgg atctgggagg agagcggcgc gttcagggcc gggcgcccgg
     2161 agcgccgcga cgcgaaaccc tttactatcg tcatcccgcc gcccaacgtg accggctcgc
     2221 tgcacatggg gcacgcgctc aacaacacct tgcaggacat tctgtgccgc ttcgaacgga
     2281 tgcgcggccg cgacgtattg tggcagcccg gcaccgatca cgccgggatc gcgacccaga
     2341 tggtggtcga gcggcagttg atggagcggc agcagccggg ccggcgcgac atgggccggg
     2401 ctaaatttct cgagcgcgtc tggcaatgga aggccgagag cggcggcgtc atcgtcaatc
     2461 agctgaagcg gcttggcgcg tcctgcgact ggtcgcgcga acgctttacc atggacgaag
     2521 gcctgtcgcg cgcggtcgtg aaggtgttcg tcgaactgca tcgtgaaggg ctgatctaca
     2581 aagacaagcg gctggtgaac tgggatccga agctgctgac ggcgatctcg gatctcgagg
     2641 tgcagcaggt cgaggtcaag ggcagccttt ggtatttgcg ctatccgctg gaaggccgga
     2701 ccttcaatcc cgacgatccc acgacgttca tcgtggtcgc tacgacgcgg cccgaaacga
     2761 tgctcggcga cagcgcggtg gcggtgcatc cggacgacga gcgctacgcc gaccttgtcg
     2821 gcaagcacgt gatcctgccg ctggtgggac gcaagattcc gattctagcc gacgaatatt
     2881 ccgacccgga aaagggctcg ggcgcggtga aggtcacgcc ggcgcatgac ttcaacgatt
     2941 tcgaagtcgg caatcggcac ggcttgccgc ggatcagcgt gctcgatcag gagggccgtc
     3001 tcacgctggt cggcaacgag gattatctgc gcggattgcc ggaagaatca gagtatttcg
     3061 cggaggaatt gaacggcctc gaccggttcg tcgcccgcaa gctgatcgtg gcacgactgg
     3121 aagatttcgg tttcctggag aggatcgagc ccaacaccca catggtgccc cacggcgatc
     3181 gctcgggcgt catcatcgag ccgtatctga cggaccagtg gtacgtcgat gccaagacca
     3241 tggcgcggcc cgcgatggca gcggtgcgca gcggcgcaac cacgttcgtg
//
LOCUS       DATNOI010000332        17005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_112891,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000332 DATNOI010000000
VERSION     DATNOI010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17005
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112891"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VN968_13155"
     CDS             <1..1011
                     /locus_tag="VN968_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXL30222.1"
                     /translation="NPFPAINRSAGHGLAIVDAKTGAPGTFMSFSTSPGAEAEDGSGA
                     DSPYTTALLSAAKEPGLSIEDTFKRVRVSVNKATEGRQTPWDSSSLTDDFRFFPGSGA
                     VAPKQVEAKRTVDQWRRELQGKRVEIANEMIVADGTDEAYEAFVGLFAQPPFGPQARE
                     WLDRHRRMVAWNNAVIINTAAGYRSFLLQYPDSDLTATARKLEDRLRNRPNIAASLAA
                     IGGAPNASAASAPAGTALPTNASLAAPTCPCGAPPLPLKKVDLPPKKRADPDPPKRAS
                     AQPPRRPRQPEDDVVVYRRPPPPDYYEPSPSPVPSIGIGIGLGGFGRGGGGYQGRSRG
                     GY"
     gene            1364..1639
                     /locus_tag="VN968_13160"
     CDS             1364..1639
                     /locus_tag="VN968_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30223.1"
                     /translation="MKVFRIAALVALLTAPAYAQTPNINLMPEFQSKSPEEKEREEAQ
                     QKAYKESLKKIPDAKASSDPWGNVRSVDTPKASAPAKPRTKTGSTAN"
     gene            complement(1773..2486)
                     /locus_tag="VN968_13165"
     CDS             complement(1773..2486)
                     /locus_tag="VN968_13165"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXL30224.1"
                     /translation="MAPPVSNAGHPLDNKLLASLPRDHFDRLLPHFTSVQLPQGLVMF
                     ESGDEVDQVYFPHYGMLSLLAVLRDGKAIETATVGREGVVGAMAGLGLYKSLVRVVAQ
                     MPVACSKIAATHFRTVATGSDPVRNLCIRYNEVLLSQARVTAACNALHSIEARFCRWL
                     LQSADRAATDTVTLTQEFLAEMLGVRRTSVTEVASKVQNAGAITYSRGVIRILDRPAL
                     LRMSCECYETLLDQSAVLG"
     gene            complement(2747..3463)
                     /locus_tag="VN968_13170"
     CDS             complement(2747..3463)
                     /locus_tag="VN968_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXL30225.1"
                     /translation="MPDDEYERLRPHLSHVIFDYRKSLYEASRPIDHVYFPIDGVASL
                     VITIANGASAEVGTIGSEGLVGLPICLGDRNAPSSVYVQVPGTALTMDARLFRGELDR
                     SPALNLIMLRYAHAFFNQVAQSAACAHLHKVEQRCCRWLLMTRDRMPSGDFLLTHEFL
                     GMMLGVRRTTVTDVMGALQKAGLVRYRRGHVTILDHEALRQRACECYEISKLEFDRLL
                     GDTAVAPRTDKRHRLISEVG"
     gene            complement(3453..3743)
                     /locus_tag="VN968_13175"
     CDS             complement(3453..3743)
                     /locus_tag="VN968_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30226.1"
                     /translation="MDQPFYLREQAERCRRLARDCTDPMLRDSLYSRSPKNTPGGPAP
                     KNITKRQFGTQAQATMTRTLTDRPTTIGAAINHERQEYARSTDEPTSLALAG"
     gene            3952..4185
                     /locus_tag="VN968_13180"
     CDS             3952..4185
                     /locus_tag="VN968_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30227.1"
                     /translation="MAFRPRDLADRMAGRRKKLDDGYLRETFTLPRDKARTRARDFLT
                     RYPKAAYMSGVESWRELPDGDIEFTMRRLRSAD"
     gene            4909..6003
                     /gene="recA"
                     /locus_tag="VN968_13185"
     CDS             4909..6003
                     /gene="recA"
                     /locus_tag="VN968_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HXL30228.1"
                     /translation="MAAPTTALRIVEGSSMDKTKALSAALSQIERQFGKGSVMKLGKN
                     DRSMDIETISSGSLGLDIALGVGGLPRGRVVEIYGPESSGKTTLALHTVAEGQKKGGI
                     CAFIDAEHALDPVYARKLGVNIDELLISQPDTGEQALEICDTLVRSGAVDVLVIDSVA
                     ALVPRAELEGEMGDALPGLQARLMSQALRKLTASINKSNTMVIFINQIRMKIGVMYGS
                     PETTTGGNALKFYASVRLDIRRIGAIKERDEVVGNQTRVKVVKNKLAPPFKQVEFDIM
                     YGEGVSKMGEILDLGVKAGIVEKSGAWFSYDSQRLGQGRENSKAFLKANPDMTAKIEA
                     AIRQNSGLIAEQILAGSPERDAEGEEPAEE"
     gene            6113..6406
                     /locus_tag="VN968_13190"
     CDS             6113..6406
                     /locus_tag="VN968_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXL30229.1"
                     /translation="MDAPFFVYILASKRNGTLYVGVTNNLARRLSEHKAKLAPGFTRQ
                     YEVDQLVYFEAFDSILEARAREHSLKRWRRAWKIALIEKLNPDWRDLTNELNF"
     gene            6624..7619
                     /locus_tag="VN968_13195"
     CDS             6624..7619
                     /locus_tag="VN968_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30230.1"
                     /translation="MTRLILTTEDSGAGCLRVAGIADIVLPFGLRFVWGPLPSDAELA
                     TFLEARSTKHGSPGSHWLDFVRARYLEEIEGKDLGLIDLCERCETIELWIDPDPNAQL
                     TLIWLLDYLRHHAKIASKLTLVQADEAIGGHLPEQVATWRLPAIRILNDHLEQASTAW
                     RAYRAPTPQDWFNLLSKDLSILPNLRRAVVKLLEELPMRATGLGATEMRMLQLLSEGI
                     VNPIGLFPHHRHQRKVFGYWEAGSLLDGLAHCPAPAVSGLGEGPFPFERYGSRTLHER
                     YARSRVSLTVLGEAILKWTEDFSRHNPISRWWGGTRLTNDSLWRWDPGYRALIAP"
     gene            complement(7771..10644)
                     /gene="gcvP"
                     /locus_tag="VN968_13200"
     CDS             complement(7771..10644)
                     /gene="gcvP"
                     /locus_tag="VN968_13200"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HXL30231.1"
                     /translation="MTAHYKPANDAAATFVRRHIGPSPRDISAMLETVGAKSLDALMG
                     ETLPSSIRQKAPLNLGPALSETEALAHMSELAARNQVFTSLIGQGYSGTILPAVIQRN
                     ILESPAWYTAYTPYQPEISQGRLEALFNFQTMICDLTGLDIANASLLDEATAAAEAMA
                     LAERAAQTKTKSFFVDREVHPQTLAVLRTRAEPLGWNLIVGDPAVDLDKAEVFGGLLQ
                     YPGTSGAVRDLHPAIAALRAKGGLVIVAADLLALTLLASPGELGADIAIGSAQRFGVP
                     MGYGGPHAAYMAVRDALKRALPGRIVGQSIDSRGQPAYRLALQTREQHIRREKATSNI
                     CTAQVLLAVIASMYAVYHGPEGLTHIARTVHRRACVLAAGLRKLGFAPQSEAFFDTVT
                     VNAGARRNEIAARARAEKINLRIGERTLGIALDETTTPDIVEAVWRAFGGRLVYADVE
                     ADTRDTLPTELKRSSAFLTHPVFHAHRSETELLRYMRKLSDRDLALDRAMIPLGSCTM
                     KLNATSEMIPLTWPAFGGLHPFAPLEQAAGYHALFARLEKWLCEITGYEAISLQPNSG
                     AQGEYAGLLAIRGYHTARGEPHRKVCLIPSSAHGTNPASASMAGMEVVVVACDGRGDV
                     DVNDLRAKAAQHSKNLAAVMITYPSTHGVFEEHIREICDVVHGHGGQVYLDGANMNAQ
                     VGLSRPGDYGADVSHLNLHKTFCIPHGGGGPGMGPIGVRAHLAPFLPGHPTTDGAKPR
                     PVGPVSAAPFGSASILTISYIYILMMSGEGLTRATEIAILNANYIATRLDAHFPVLYR
                     NAKGRVAHECIVDPRPLKISSGVTVDDIAKRLIDYGFHAPTMSFPVPGTLMIEPTESE
                     SRAELDRFCDAMIAIRREIAEIESGRWKIEASPLRHAPHTVHDIADDSWARAYSRTEG
                     CFPSGTSRVDKYWCPVGRVDNVYGDRNLVCSCPPVEDYAQAAE"
     gene            complement(10793..11158)
                     /gene="gcvH"
                     /locus_tag="VN968_13205"
     CDS             complement(10793..11158)
                     /gene="gcvH"
                     /locus_tag="VN968_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HXL30232.1"
                     /translation="MTTLFTSDHEWLRIEGDVATIGVTDYAQSQLGDVVFVELPKVGR
                     SLKKAEAAAVVESVKAASDVYAPISGEVTEVNDALSAEPALVNSDAAGKAWFFKLKIA
                     DRSELDGLMDEAAYKAHTA"
     gene            complement(11169..12314)
                     /gene="gcvT"
                     /locus_tag="VN968_13210"
     CDS             complement(11169..12314)
                     /gene="gcvT"
                     /locus_tag="VN968_13210"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HXL30233.1"
                     /translation="MLARDDQSELKRTPLHALHVASGGKMVPFAGYDMPVQYASGVLR
                     EHLHTRSHAGLFDVSHMGQIALRPKSGRVEDAALALEQLVPQDILAVAPGRQRYAQFT
                     NGAGGILDDLMVANFGSHLFLVVNAACKAEDEAHLRANLSETCVIEPLPDRALIALQG
                     PKAESVLAKLCADVALMRFMDAGPRSVDRLDGFVSRSGYTGEDGFEISVPAEHAERLA
                     KALLGNGDVLPIGLGARDSLRLEAGLCLYGHDIDATTTPVEGALEWSIQKSRRRGGAR
                     AGGFPGADQILSQLENGAARRRVGLRPEGRAPVREGAPLFPDAASSEQIGKVTSGGFG
                     PSINAPVAMGYLPSSHAIGGLVFAELRGQRLPLRVAATPFVPNTYKR"
     regulatory      complement(12452..12542)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            12751..15435
                     /gene="alaS"
                     /locus_tag="VN968_13215"
     CDS             12751..15435
                     /gene="alaS"
                     /locus_tag="VN968_13215"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HXL30234.1"
                     /translation="MSGVNEIRSAFLNYFAKNGHELVPSSPLVPRNDPTLMFTNAGMV
                     QFKNVFTGIEKRPYQRATTSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KDRAIELAWNLVTKEFGLPKDKLTATVYVDDDEAFDLWKKIAGLPESRIIRIAGSDNF
                     WQMGDTGPCGPCSEIFYDHGDKIWGGPPGSAEQDGDRFIEIWNLVFMQFEQIEPGVRN
                     PLPKPSIDTGAGLERVAAVLQGKHDNYDIDLFVALIRAISELTGADPNGEQKASLRVI
                     ADHLRASSFLISDGVLPSNEGRGYVLRRIMRRAMRHAQLLGAKDPLMHRLVWALVREM
                     GQAYPELVRAENLIEETLRLEETRFRKTLERGLSILDEKSAALKKGDMFDGDTAFTLY
                     DTYGFPLDLTQDALRNRGIGVDIASFTDAMERQRAKARAAWAGSGDTASENVWFPLRE
                     KLGATEFLGYETESAEGVVAALVKDGKDVESLKAGEAGAIVLNQTPFYAESGGQVGDT
                     GMLTGEGVRFRVTDTQKKAGDLFVHLGNVEQGTLKPGTPLQLEVDHARRSSIRAHHSA
                     THLLHEALRQVLGDHIAQRGSLVAPDRLRFDFVHPKPITPEELSRIEDIANDVVLEND
                     EVTTRLMAVDDARDAGARALFGEKYGDEVRVVSMGKTDREHGSNALGWSVELCGGTHV
                     RRTGDIGLISVTGESAVASGVRRIEALTGNHARKHANETMALAKSAATELRTTLDDMP
                     ARIASLMEERKKLERDLSEARKKLAMGGGAQASHGSAAGANGVREVGNVKLMARAVEG
                     IEMKDLKSLVDDGKKQLGSGVVAIVAVTEDGKAGVVVGVTSDLTSRYNAVDLVRVASE
                     ALGGKGGGGRPDMAQAGGPDGAKAAAALAAIEKAMGSA"
     gene            15438..16685
                     /locus_tag="VN968_13220"
     CDS             15438..16685
                     /locus_tag="VN968_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-gated ion channel"
                     /protein_id="HXL30235.1"
                     /translation="MATAPSRRRLSDPDLRDRLYELLEHDHLPYSVGSRFVRLIVCII
                     VIDVMAMILASVPEFDARFGVVFTVIKIGAVAAFGLEYAARLWSVAGHSPRKLSQSRD
                     RIEYAFSSLGIIDLLAFLPASIALIAGSRSTLVLLGMLPFLKLVRYSPAMRSLLSALH
                     AERRTLIGCLVILAGAVLLFASLLYAIEHDVQPDKFGTIPHAMWWAIVTLGTVGYGDV
                     VPVTPLGKLVSVFTIVGGLTMIALPVAIISTAFADEVRRRDFVVTWGMLARVPLFSHL
                     SAAEIADIMRLLRAHTIESGEILVRRGDAASSMYFITAGEVEIELPTQRVRLSDGTFF
                     GEIALLHRTKRSGTVTATRKTKLLALDAQDFHALIERVPDLAAHVHRTAKARLADTAQ
                     AQKGDLAAGEIAQAENADGDAGG"
BASE COUNT         3167 a         5226 c         5486 g         3126 t
ORIGIN      
        1 aacccattcc ccgcgatcaa ccgcagcgca ggccacgggc tggcgatcgt cgatgcgaag
       61 accggcgctc ccggcacgtt catgtcgttt tcgacgtcac ctggtgccga agccgaggac
      121 ggcagcggcg ccgacagtcc ctatacgaca gcgctgcttt cggcagccaa agagcccggg
      181 ctttcgatcg aagatacctt caagcgcgtc cgggtttcgg tcaacaaggc gaccgaggga
      241 cgccagacgc cctgggatag ttcctcgcta accgacgatt tcagattctt tcccggatcc
      301 ggcgcggtgg ccccgaagca agttgaggcc aagcgcaccg tcgatcaatg gcgtcgtgaa
      361 ttgcagggca agcgggtcga gatcgccaat gaaatgatcg tcgctgatgg caccgacgag
      421 gcctatgagg cgtttgtcgg tttgttcgcg caaccgccgt tcggcccgca ggcccgtgaa
      481 tggctcgatc gtcatcgccg gatggtggcg tggaacaatg cggtgattat caacacggcg
      541 gcgggatatc gctcattcct tctccagtac cccgatagcg atctcaccgc gacagcgcgc
      601 aagctggaag accgcctgcg caatcgcccc aacatcgcag ccagcctggc cgccatcggc
      661 ggcgcgccga acgcgtcagc cgcgtccgca ccggccggaa ctgctctgcc gaccaacgcc
      721 tcgcttgccg ctccgacatg cccctgtggc gcgccacccc tgccgctgaa gaaagtcgat
      781 ctgccgccca agaagcgcgc cgatcccgat ccgcccaagc gcgccagcgc gcaaccgccg
      841 cggcgtccgc gtcagccgga agacgatgtc gtggtctacc gtcgcccgcc gccgcccgac
      901 tattacgaac catcgccatc gccagtaccc tcgattggca tcggaatagg cttgggaggg
      961 ttcggacggg gcggaggcgg ctatcagggc agatcgaggg gcggatactg attgcggaac
     1021 ctgcgggcgc tcacatgggt gcgggtggct catccagggc gcatttgatg gcaatggacg
     1081 gcgccccgcg tagcttgaaa atgccaaact cgaggccgaa taataggggg cggggagcag
     1141 gtcgaccatg ttgacgcgat tggcgctgat tttcagcgtg atcgccgtgg atcttgccct
     1201 caacggctgc accagatgcg gtccgatcta gacgattcga cgcaatcgcc gaagtcctgc
     1261 gccgaagtcc tgcaagtcgg gccatctttg gcgcgcaagc tgctacgctt ggcgtcgagg
     1321 tcggcgcggt ggtatatcga tgggaagatg cagggagcgt tccatgaaag tttttcgcat
     1381 agccgcgctg gtggcgttgc tgacagcgcc ggcttacgca cagactccca acatcaattt
     1441 gatgccggag tttcaatcga aaagtcctga ggaaaaggag cgggaggagg cccagcaaaa
     1501 ggcctacaag gaatcgctga agaaaatacc ggacgccaag gcctcttcgg atccctgggg
     1561 caatgtgcgt agcgtggata cgcccaaggc atcagcccct gcaaagccgc ggaccaaaac
     1621 cggcagcacc gcgaattaag acgaaatggt tgaacgagga tgcgaacgcc ggcgaggctg
     1681 gcgggcgtcc gcggctttcg gacgagggca gggagcctta tttcctgctg ctgatgtttg
     1741 tcagctcaag cagcggggct tgcgcggaaa aatcagccca gtaccgccga ttggtccagc
     1801 agcgtttcat agcactcgca tgacatccgc agcagcgcgg gccggtccag gatcctgatc
     1861 acgccgcgcg aataggtgat cgcgcctgcg ttttgcacct tgctcgcgac ttccgtcacc
     1921 gaggtgcgcc gcacgccgag catttcggcg aggaactcct gtgtcagcgt gaccgtgtcg
     1981 gttgcggccc gatccgcgct ctgcagcagc cagcggcaaa atcgcgcctc gatcgagtgc
     2041 agcgcattgc aggcggccgt cacccgcgcc tgcgacaaca gcacctcgtt gtagcggatg
     2101 cagagattgc ggacgggatc gctgccggtg gcgacagtcc tgaaatgggt ggcggcgatc
     2161 ttgctgcagg ccaccggcat ctgcgccacg acgcgaacca gcgacttgta caggccgagc
     2221 cccgccatcg cgccgaccac gccttcgcgg ccgacggtcg cggtctcgat cgccttgccg
     2281 tcgcgcagca cggcgagcag cgacagcatg ccgtaatgcg ggaagtaaac ctgatcgacc
     2341 tcgtcgccgg attcgaacat caccagcccc tgcggcagtt gcacggacgt gaagtgcggc
     2401 agcagccgat cgaagtgatc gcgcggcagc gaggcgagga gcttattgtc caggggatga
     2461 ccggcgtttg agacgggtgg tgccactgcg atatcctgcc cggtcggtcg ggccgattta
     2521 ccgcaggggt gaaggacgct ccgagagggc gatccgcggg aaggctcatg tataggaaca
     2581 tgaccgggca ctgtcggtcc ggtagccgtc atagcgtcgg tttccggggc gcgttatctc
     2641 gcttgaatgg caactgcgcc gcaaggtttc tggtagcagg ggccgttttc gcggattgaa
     2701 atctattgtc ggaaaacgga ctgagctgat gacggattta tgttttctat ccgacttcgc
     2761 tgatcaatcg atgcctctta tcggtccttg gcgcgacggc ggtatcgcca agcagccgat
     2821 cgaattccag tttcgagatc tcgtagcatt cgcaggctcg ttgccgcaaa gcctcgtggt
     2881 cgaggatcgt gacgtgcccc cgcctatacc ggacaagccc ggctttttgc agggcgccca
     2941 tcacatcggt gaccgtggtt cgccgcacgc ccagcatcat ccccaggaat tcatgggtga
     3001 gcaaaaagtc gccggatggc atccggtcgc gggtcatcaa cagccagcgg caacagcgct
     3061 gctctacctt atgcaggtgg gcgcaggctg ccgactgcgc gacctggttg aagaaggcat
     3121 gggcatagcg gagcatgatc aggttcaatg ccgggctccg gtcgagttcg ccgcggaaga
     3181 ggcgggcgtc catggtgaga gccgtcccgg gaacctgcac ataaaccgat gaaggtgcat
     3241 tgcggtcccc gagacaaatc ggcaatccca ctaatccttc gctgccgatg gtgccgacct
     3301 ccgcgctggc accattggct atcgtgatca ccagggacgc cacgccgtcg atcgggaaat
     3361 acacatggtc gatcggacgc gaagcctcat agaggctttt tctgtaatcg aaaatgacat
     3421 gcgacaaatg cggccgcagc cgctcgtatt cgtcatccgg caagagcgag agaagtcggt
     3481 tcgtccgtcg atcgggcata ctcttgtctc tcgtggttga tggcggcgcc aatcgttgtt
     3541 ggccggtcag tcagcgtcct ggtcatcgtc gcctgagcct gcgtgccaaa ctgccgtttc
     3601 gtgatattct tgggcgccgg cccgcccggt gtattcttcg gcgagcgtga ataaagactg
     3661 tcgcgcaaca ttggatcggt gcaatcgcgg gccagacggc ggcaacgctc ggcctgttcg
     3721 cgaaggtaga aaggctgatc cataggactc gctcgcaacg aggcgggagt cccccctttt
     3781 ggggctctca gtcgccggcg ccctagagtt attgccgaca atgctgaaat tataccgatt
     3841 ttatgtccgg taagcgactc aatttctcgc ccgcccgccg gtgtggcggg actcggatcg
     3901 gctctcgcaa gcgctatatt aagccggtcg atacctgtct ggagttgcga catggccttt
     3961 cgcccgcgcg atcttgccga ccggatggcc ggccgacgga agaagctcga cgacggctat
     4021 ctgcgcgaga ccttcaccct gcctcgcgac aaggcgcgga ccagggcgcg cgacttcctc
     4081 acccgctacc ccaaggccgc ctatatgagc ggggtcgaaa gctggcgcga actacccgat
     4141 ggcgacatcg aattcacgat gcgacggctg cgcagcgccg actagcaatc cgaagttccc
     4201 tcgcatcgcc cgaaggcgcg ggtggacgcg ggccgacaca tggcgccggc tactcctctt
     4261 tttcaccgag caacctgtgg ctcgatcgat tgatgaaatg aagccggcca caggcctggc
     4321 agagcaccgt ttcgtaagag caacgctgat cgccgggcac gggctcgtcc gccagccgat
     4381 gctgcacgtt catgccggtc cggggacatt tgaagacgaa gttgctcatg caactggtca
     4441 gtctgacgac tgcacggcgt tccgtcttga tccctgtcaa ttccggagcg ggaggatatg
     4501 ccaccttcaa gttgcatctg ttcgatattg ctctctctct ctctgctgct gcacggattc
     4561 aagggaagtg gcctgccgag tggggctcaa tgcgcgccag cgtgatgcgc tgcgggtaac
     4621 gcacgcacgc aagcgttccg tcagccaaca ctacgtaatt ctacggagat tggtgggtcc
     4681 agaattatcg gtttgttaac agcaacgaac ccgccaacaa cttaaaattg caccactaag
     4741 ggtagcggtg cacgttattc acaggcggga agaaagaaca tattgcgaac gaagaacaaa
     4801 tgaggtacgg tgttccatca gatccagttg atgaccagac aaagtagctg gtccgttgtt
     4861 gccaccgttt gtcccgctag cgaatccccg ccataaggag caccaccaat ggccgccccc
     4921 accactgccc tgcgtatcgt cgaaggatcc tccatggaca agaccaaggc cctgtccgcc
     4981 gcgctctccc agatcgagcg ccagttcggc aagggctcgg tgatgaaact gggcaaaaac
     5041 gaccgctcga tggacatcga gaccatttcc tcgggctcgc tcgggctcga catcgcgctc
     5101 ggcgtcggcg gcttgccgcg cggccgggtg gtggaaattt atgggccgga atcctcaggc
     5161 aagaccacgc tggcgctgca caccgttgcc gagggacaga agaaaggcgg catctgcgcc
     5221 ttcatcgacg ccgaacacgc gctcgacccg gtctatgccc gaaagctcgg cgtcaatatc
     5281 gacgagctct tgatctcgca gcccgacacc ggcgagcagg cgctggaaat ctgcgacacc
     5341 ctggtgcgct ccggcgcggt cgacgtgctg gtgatcgatt cggtggcggc cctggtgccg
     5401 cgcgccgaac ttgaaggcga gatgggcgat gccttgcccg gcctgcaggc gcggctgatg
     5461 agccaggcgc tgcgcaagct caccgcctcg atcaacaaat ccaacaccat ggtgatcttc
     5521 atcaatcaga tccggatgaa gatcggcgtg atgtacggct cgccggagac caccaccggc
     5581 ggcaacgcgc tgaagttcta cgcctcggtc cggctcgaca tccgccgcat cggcgcgatc
     5641 aaggagcgcg acgaggtggt cggcaaccag acccgcgtca aggtggtgaa gaacaagctg
     5701 gcgccgccgt tcaagcaggt cgagttcgac atcatgtatg gcgagggcgt ctccaagatg
     5761 ggtgaaattc tcgatctcgg cgtcaaggcc ggcattgtcg agaaatccgg cgcctggttc
     5821 tcctatgaca gccaaaggtt ggggcagggc cgcgagaact cgaaagcgtt tctcaaggcc
     5881 aatcccgaca tgaccgccaa gatcgaggcg gcgatccgcc agaactccgg cctgatcgcc
     5941 gaacagatcc tggccggctc acccgagcgc gacgccgaag gcgaggagcc ggcggaggag
     6001 tgatcttcac tcgtgtcccg gacgcggtgc agcgttcttc acgatgcacc gcagagccgg
     6061 gacccacggc ttggcatcaa aatcccattg ctgatccgag gcatgagagg caatggacgc
     6121 gccgttcttt gtctacattc tcgcaagtaa gcgaaatggg acgttgtatg tcggcgtcac
     6181 caacaatctc gctcggcgtt tgtcggagca taaagccaag cttgctcccg gtttcacacg
     6241 gcaatatgaa gtcgatcaac tcgtctattt tgaagccttc gattccatcc tagaagcgcg
     6301 cgcgagagaa cattccttga agcgctggcg ccgcgcatgg aagatcgcct tgatcgagaa
     6361 gctcaacccg gattggcgtg atctgaccaa cgaattaaat ttctgagaca tctccgggac
     6421 gcttggcgtg ccccggatgc tgcgcagcgc gccgcagtag cggcgtggtg cgctgctgat
     6481 ccggggccca tgcgcatgat aaagtgtggg tcccggctct gcggtgcacc gtgaagaacg
     6541 ctgcaccgcg tccgggacac gatcttatcc acgcgcgagg tgctcggtga ggggtgatgc
     6601 agtcttggct attcggtgac gatatgacgc gtttgatcct gacgacggaa gactcggggg
     6661 ccggatgcct cagggtggcc ggtatcgctg acatcgtgct tccgttcggc cttcggttcg
     6721 tctggggacc gctgccgtcc gatgcggagc tcgcaacgtt cttggaggcg cgctcgacga
     6781 aacacggctc tccgggctcc cactggctgg attttgtccg cgcgcggtat ctcgaagaaa
     6841 tagaaggaaa agacctcgga ttgatcgacc tctgcgagcg gtgcgagacg atcgagttat
     6901 ggatcgatcc ggacccaaat gcccaactaa cgctgatctg gctgctggac tatttgcggc
     6961 atcacgcgaa gatcgcttcg aagctgactt tggtccaggc ggacgaggcc atcggcggtc
     7021 acctgcccga gcaagtggcg acatggcggc tgccggccat cagaattctc aacgatcatc
     7081 tggaacaagc cagcacagcg tggcgggcct atcgagcccc gaccccccag gactggttca
     7141 acctgctgtc aaaggactta agcatcctgc cgaatcttcg gcgggcggtt gtgaagctac
     7201 ttgaagaact tcccatgcgc gcaacggggc ttggcgcgac cgaaatgcgg atgcttcagc
     7261 tcttatcgga aggtatcgtg aatcctatcg ggctctttcc gcaccaccgc caccagcgaa
     7321 aagtgttcgg ctattgggag gccggatcgc tgctggacgg gttggcgcat tgtccggcgc
     7381 ctgctgtatc cggcctcggc gaagggccgt tcccgtttga gaggtacggc tcccgtaccc
     7441 ttcacgagcg atacgcacga agtcgcgtat cactcaccgt actcggcgag gctatcttga
     7501 aatggactga agacttcagc cggcacaacc cgatttcccg ctggtggggc gggactagat
     7561 tgacgaatga cagcctgtgg cgctgggatc cggggtaccg cgctttgatc gcgccgtagt
     7621 ggcgagccgt tttgtttttg ctgcaagggg cattctcagc cgtcatgccc gggcttggtc
     7681 ccgggcatcc acgtcctacc cgctcggatg cttaaagacg tggatggccg ggagaaaccc
     7741 ggccatgacg aattggggtc tgctatcccg tcactctgcg gcctgtgcgt aatcctcgac
     7801 cggcggacac gagcacacca gattgcggtc gccatagaca ttgtcgacgc gtccgaccgg
     7861 acaccagtat ttgtcgacgc gtgaggtgcc tgacggaaag cagccctcgg tgcggctata
     7921 ggcccgcgcc cagctatcgt cggcgatgtc gtgcacggtg tgcggggcgt ggcgcagcgg
     7981 tgaggcctct attttccagc ggccagactc gatctcggcg atctcgcgcc ggatcgcgat
     8041 catggcatca caaaaacggt ccagctcggc cctggattcg gattcggtcg gctcgatcat
     8101 cagcgtgccc ggcaccggaa agctcatggt cggcgcatgg aagccgtaat cgatcagccg
     8161 cttggcgata tcgtcgacgg tgacgccgct cgagatcttg agcgggcgtg gatcgacaat
     8221 gcattcatgc gcgacgcggc ccttggcgtt gcggtacagc accggaaaat gggcatcgag
     8281 cctggtcgcg atgtagttgg cgttgaggat cgcgatttcg gtcgcgcgcg tcaggccttc
     8341 gcctgacatc atcagaatgt agatgtagga gatggtcagg atcgatgccg agccgaacgg
     8401 cgccgccgac accgggccga ccggccgcgg cttggcgccg tcggtcgtgg ggtgccccgg
     8461 caggaacggc gccaaatgcg ctcttacgcc gatcggcccc attcccggac cgccgccgcc
     8521 atggggaatg cagaacgtct tgtgcaggtt gagatggctg acatcggcgc cgtaatcgcc
     8581 gggccgcgac agcccgacct gcgcgttcat gttggcgccg tcgagataga cctgtccgcc
     8641 atggccatgc acgacgtcgc agatctcgcg gatatgctcc tcgaatacgc cgtgggtgga
     8701 gggataggtg atcatgacgg cggcgagatt tttcgaatgc tgcgccgcct tggcgcgcag
     8761 gtcattgacg tcgacgtcgc cgcgtccatc gcacgccacc accacgacct ccatgccggc
     8821 catgctggcc gaagccggat tggtgccgtg cgcggacgaa ggaatcaggc agaccttgcg
     8881 atgcggctcg ccgcgcgcgg tgtgatagcc gcggattgcg agcaggccgg catattcgcc
     8941 ctgcgcgccg gagttcggct gcagcgagat cgcctcatag ccggtgattt cgcacagcca
     9001 tttttcaagc cgcgcgaaca gcgcgtgata gccggccgcc tgttcgagcg gtgcgaacgg
     9061 atgcaagccg ccgaacgcgg gccaggtcaa cgggatcatc tccgaggtgg cgttgagctt
     9121 catggtgcag gagcccagcg gaatcatggc gcggtcgagc gccagatcgc ggtcggacag
     9181 cttgcgcatg taacgcaaga gttcggtctc ggagcgatgg gcgtgaaata ccggatgggt
     9241 gaggaacgcg cttgagcgct tcagctcggt gggcagtgta tcgcgggtat cggcttcaac
     9301 gtccgcgtag acaagccggc cgccgaatgc gcgccacacc gcctcgacga tgtcaggtgt
     9361 cgtggtttcg tccagcgcga tgccgagcgt gcgctcgccg atccgaaggt tgattttttc
     9421 ggccctggcg cgggcggcga tctcgttccg ccgcgcgccg gcgttcaccg tcaccgtatc
     9481 gaaaaacgcc tcggattgcg gcgcgaaccc aagcttgcgc aatcccgccg ccagcacgca
     9541 agcacggcga tgcaccgtgc gcgcgatgtg ggtcagtcct tccggaccgt gatagactgc
     9601 gtacatcgag gcgatgaccg ccagcagcac ctgcgcggtg cagatgttgg aggtcgcctt
     9661 ttcgcggcgg atgtgctgct cgcgggtctg cagcgccaga cgataggcgg gctgcccccg
     9721 cgaatcgatc gactgcccga cgatgcggcc gggcagcgcg cgtttcagcg cgtcgcgcac
     9781 cgccatatag gccgcgtgcg gaccgccata tcccatcggc acgccaaaac gctgcgccga
     9841 tccgatcgcg atatcggcgc cgagttcgcc gggcgaggcc aaaagcgtca gcgccagcag
     9901 atcggcggcg acaatcacca gcccgccttt ggcgcgcagc gccgcgatgg cgggatgcag
     9961 gtcgcgtacc gcgccggaag tccccggata ctgcagcagc ccgccgaaca cctcggcctt
    10021 gtcgagatcg acggcgggat cgccgacgat caaattccac cccaacggtt cggcgcgggt
    10081 gcgcagcaca gcgagcgtct gcggatgcac ctcgcggtcg acgaagaatg attttgtctt
    10141 ggtctgtgcg gcgcgctcgg cgagcgccat cgcttcggcc gccgcggtag cctcatcaag
    10201 caacgaggca ttggcgatat cgaggccggt caggtcgcag atcatggtct ggaagttgaa
    10261 taaagcttcc agccggccct ggctgatctc cggctgatac ggcgtgtagg ccgtgtacca
    10321 cgccggactc tccaggatat tgcgctggat caccgccggc aaaatcgtgc cggaatagcc
    10381 ttggccgatc agcgaggtga agacctggtt tcgtgccgca agctcgctca tatgcgccag
    10441 cgcttcggtt tcgctcagcg ccgggccgag attgagcggc gccttctgcc ggatcgacga
    10501 cggcagggtt tcgcccatca gcgcatcaag gctttttgcg cccaccgttt cgagcatcgc
    10561 cgatatatcg cggggggagg ggccgatgtg gcggcgcacg aaggtggcgg cggcgtcatt
    10621 ggcgggtttg tagtgcgcgg tcatgggttt gtcctagcta ttcgtcatgc ccgcgaaggc
    10681 gggcatccag taaacgttga cgtcggtgat ggaaaccgag gcgtcccggc gtactggatc
    10741 gtccgccttc gcggacgatg acgctgtggg ggttgtggct ctcttggtca tctcacgccg
    10801 tatgcgcctt gtacgcggcc tcatccatca ggccgtcgag ttcgcttctg tctgcgatct
    10861 tcagcttgaa gaaccacgcc ttgccggcgg cgtcggaatt gaccagtgcc ggctcggctg
    10921 acagcgcgtc gttgacttcc gttacttcgc ctgaaatcgg cgcgtagacg tcggatgccg
    10981 ccttgaccga ttcgaccacg gcggcggctt ccgccttctt caggctgcgg ccgaccttcg
    11041 gcaattcgac gaacacgacg tcgccgagct gcgactgcgc gtagtcggtg acgccgatgg
    11101 tggcgacatc gccctcgatg cggagccatt cgtggtctga tgtgaacaac gtcgtcattg
    11161 caaatcctct agcgtttgta ggtattggga acgaaggggg tggctgcgac ccggagcggc
    11221 agccgctggc cgcgcaattc ggcgaacacc aggccgccga ttgcatgaga agagggcaga
    11281 tagcccatcg cgaccggtgc gttgatgctg gggccaaagc cgcccgaggt gaccttgccg
    11341 atctgttccg acgaagctgc gtcaggaaac agcggtgcgc cttcgcggac cggggcgcgg
    11401 ccctccggcc tcaatccgac gcggcggcgc gcggcgccgt tctcaagctg cgagagaatc
    11461 tggtccgcgc ctggaaaacc gccggcgcgg gcgccgccgc ggcggcggct cttctggatc
    11521 gaccattcca gcgcgccctc gaccggcgtg gtcgtcgcgt cgatgtcgtg gccatagaga
    11581 caaagtccgg cctcgagccg caggctgtcg cgcgccccta gcccgatcgg cagcacatcg
    11641 ccgttcccaa gcagcgcctt cgcaagccgc tcggcgtgtt cggccggaac cgaaatctca
    11701 aagccgtcct cgccggtgta gcccgagcgc gagacgaagc catcaaggcg atcgacgctg
    11761 cgcggaccgg cgtccatgaa ccgcatcaaa gcgacgtctg cacaaagttt cgccagtacc
    11821 gattccgcct tcggtccctg cagcgcgatc agcgcgcgat cgggaagcgg ctcgatcacg
    11881 catgtctcgg acaggttggc gcgcagatgc gcctcgtcct cggccttgca ggcggcgttg
    11941 accaccaaaa acaggtggct gccgaaattc gccaccatca gatcgtcgag aatgccgccg
    12001 gcgccgttgg tgaactgcgc gtagcgctgc cgtcccggcg caaccgccaa aatatcctgc
    12061 ggcaccagct gctccagcgc gagagccgcg tcctcgaccc tgccggattt cgggcgcagc
    12121 gcgatctggc ccatatgcga aacgtcgaac aggccggcat gagagcgggt atgcaggtgc
    12181 tcccggagca cgccgctggc gtattgcacc ggcatgtcat agcccgcgaa cggcaccatc
    12241 ttgccgccgg aggcgacatg cagcgcatgc aagggggtgc gtttcagctc ggattggtca
    12301 tcgcgcgcaa gcatcatgga gccctcgcgg tttttcgagg ctgattcctc gaaaacctgt
    12361 cgaagcccca tctgtcgctg tgcctgagag tattatcccg tcggcggaca cgtccgggcg
    12421 tcagcccggc ggtgtctctt tccagatgct atcaagtcac gcggtccgtt tgcctgagag
    12481 tttccggggc ggttgctcct tcggcgccgg ctcctgagcc gatctctccc agcgtgactg
    12541 tcttgaacag atgggaggaa aacacggctg cgccaagcct gtcaacgcgg ctcacgccga
    12601 tatcaacggt tcctgtgctg ctgcggcaag ctcctgatcc cgtgcacact ttctggacac
    12661 cgcaagcccc cacgtctaaa agcgtttgcg ccggtaaaat gacccccgct agctgctggt
    12721 cccgggcgtt ctcgattgga ttgaactgat atgagcggcg ttaacgaaat cagatcggcg
    12781 tttctgaact attttgcgaa gaatgggcac gaactcgtgc cgtcgtcgcc gctggtgccg
    12841 cgcaacgacc cgacgctgat gtttaccaat gccggcatgg tgcagttcaa gaatgtcttc
    12901 accggcatcg agaaacgtcc gtatcagcgc gccaccacgt cgcagaaatg cgtgcgcgcc
    12961 ggcggcaagc acaacgatct cgacaatgtc ggctacaccg cgcggcatca cacgtttttc
    13021 gagatgctcg gcaacttctc atttggcgat tatttcaagg atcgcgccat cgagttggcg
    13081 tggaatctgg tgacaaaaga attcggcctg ccaaaggaca agctgacggc gaccgtctat
    13141 gtcgatgacg acgaggcgtt cgatctgtgg aagaagatcg caggcctgcc ggagtcgcgc
    13201 atcatccgca tcgcgggatc agacaatttc tggcagatgg gcgacaccgg cccgtgcggg
    13261 ccttgctcgg aaatcttcta cgatcacggc gacaagatct ggggcggacc gccgggttca
    13321 gccgagcagg acggcgaccg cttcatcgag atctggaatc tcgtgttcat gcaattcgaa
    13381 cagatcgaac cgggcgtgcg caatccgctg ccgaaaccgt cgatcgatac cggtgcgggc
    13441 ctggagcgtg tcgcggccgt gttgcagggc aagcacgaca attacgacat cgatctgttc
    13501 gtagccttga tccgcgccat ctcggagctg accggcgccg atccgaatgg cgagcagaaa
    13561 gcctcattgc gcgtcatcgc cgatcatctg cgtgcgtcgt cgttcctgat ttccgacggc
    13621 gtgttgccct cgaacgaagg ccgtggctat gtgctgcgcc ggatcatgcg ccgcgcgatg
    13681 cgccatgcgc agttgctcgg cgccaaggac ccgctgatgc atcgcctggt ctgggcgttg
    13741 gtgcgcgaga tgggccaggc ctatcccgaa ctggtgcgcg ccgaaaatct gatcgaggaa
    13801 acgctgcggc ttgaagagac ccggttccgc aagacgctgg agcgcggcct gtccattctc
    13861 gacgagaaga gcgccgcctt gaaaaaaggc gacatgttcg acggcgacac ggcgtttacg
    13921 ctgtacgaca cctatggctt tccgctcgat ctcacccagg acgcgctgcg caaccgcggc
    13981 atcggcgtcg atatagcatc cttcactgac gcgatggagc ggcagcgcgc caaggcgcgc
    14041 gcggcatggg cgggctccgg cgataccgcc agcgaaaacg tgtggtttcc attgcgcgaa
    14101 aaactcggcg ccacggaatt cctgggctat gagaccgaga gcgccgaggg cgtggtggcg
    14161 gctttggtga aggacggcaa ggatgtcgaa agcctcaagg ctggcgaggc cggcgccatc
    14221 gttctgaacc agacgccgtt ttatgcggag tccggcggcc aggttggcga caccggcatg
    14281 ttgaccggcg agggcgtgcg gtttcgtgtc accgataccc agaagaaggc gggcgatctg
    14341 ttcgtgcacc tcggtaacgt ggagcagggc acgctgaagc cgggcacgcc gctgcagctc
    14401 gaagtcgatc atgccaggcg gagctcgatc cgcgcccatc actccgcgac gcatctgctg
    14461 cacgaagcct tgcggcaggt gctcggcgac cacatcgcgc agcgcggctc gctggtggcg
    14521 ccggaccggc tgcgcttcga cttcgtgcat cccaagccga tcacgccgga ggaattgagc
    14581 cgcatcgagg atatcgccaa cgacgtggtg ctggaaaatg acgaggtcac caccaggctg
    14641 atggcggtgg acgatgcccg cgacgccggc gcccgggcgc tgttcggcga aaaatacggc
    14701 gacgaggtgc gcgtggtatc gatgggcaag accgatcggg agcacggcag caacgcgctc
    14761 ggctggtcgg tggaattgtg cggcggcacc catgtgcgtc gcaccggcga tatcggcctg
    14821 atctcggtca ccggcgagag cgcggttgcc tcaggcgttc gccgcatcga ggcgctgacc
    14881 ggcaaccatg cgcgaaagca tgccaacgaa accatggcgc tcgccaagtc agcggcgacg
    14941 gagctgcgca ccacgctcga cgacatgccg gcgcgcatcg cctccctgat ggaagagcgc
    15001 aagaagctgg agcgcgacct gtcggaggcg cgcaagaagc tggcgatggg cggcggggcg
    15061 caggcctccc acggatcggc ggcgggcgcc aatggcgtgc gcgaggttgg caacgtcaag
    15121 ctgatggcgc gcgcggtcga gggcatcgag atgaaagacc tcaagagcct cgtcgatgac
    15181 ggcaagaagc agctcggctc gggtgtggtc gccatcgtgg ccgtcaccga ggatggcaag
    15241 gccggggtcg ttgtcggcgt caccagcgat ctcacctcgc gctacaatgc ggtcgatctg
    15301 gtgcgcgtcg cctccgaagc gctcggcggc aagggcggcg gcggacgacc cgacatggcg
    15361 caggccggcg ggcccgatgg cgccaaggcc gcggcggcgc tcgcggcgat cgaaaaagcg
    15421 atgggtagcg cctgacggtg gcgaccgccc caagcaggcg aagactgagc gacccggatt
    15481 tgcgggatcg gctctacgaa ctgcttgagc acgatcacct gccttattcg gtcggctcgc
    15541 gcttcgtccg gctgatcgtt tgcatcatcg tcattgacgt catggcgatg atcctggcgt
    15601 cggtgcccga gttcgacgcg cgtttcgggg tagtgttcac cgtcatcaaa attggcgcgg
    15661 tggcggcgtt tgggctggaa tatgccgcgc ggctgtggag cgtagcgggt cattcgccgc
    15721 ggaaattgtc gcagtcgcgg gaccgcatcg aatacgcgtt ctcaagcctc ggcatcatcg
    15781 acctcctggc attcttgccg gcgtcgattg cgctgatcgc cggcagccgg tcgacgctgg
    15841 tgctgctcgg catgctgccg ttcctcaagc tcgtgcgtta ttcgccggcg atgcgctcgc
    15901 tgctgtcggc cctccatgcc gaacggcgaa cgctgatcgg ctgccttgtg attctcgcag
    15961 gtgcggtgct gctgttcgcc tcgctgcttt acgccatcga gcacgacgtg cagcccgaca
    16021 agttcggcac cattccccac gcgatgtggt gggccatcgt gacgctgggc acggtcggct
    16081 acggcgatgt cgtgcccgtg acgccgctcg gcaagctggt gtcggtattc accatcgtcg
    16141 gcggattgac gatgatcgcg ctgccggtgg cgatcatctc gactgcattt gccgacgagg
    16201 tgcggcggcg cgatttcgtg gtcacctggg gcatgctggc gcgggtgccg ctgttctcgc
    16261 atcttagcgc cgctgaaatc gccgacatca tgcggctgtt gcgggcccat accatcgagt
    16321 cgggcgagat cctggtacgg cgcggcgatg ccgcgtcctc gatgtatttc attaccgcgg
    16381 gggaggtcga gatcgagttg ccgacccagc gggtccggct ttcggacggc acgttctttg
    16441 gcgaaatcgc gctgctgcat cgtaccaagc gcagcggcac cgtgacggcg acgcgaaaaa
    16501 caaaactgct ggcgctcgat gcccaggact ttcacgcgct gatcgagcgc gtgcccgatc
    16561 ttgccgccca tgttcacagg accgcgaagg cgcggcttgc cgataccgcg caagcgcaga
    16621 agggcgatct cgccgccggt gaaatcgcgc aagccgagaa cgcggacggc gacgccggcg
    16681 gctgaagtct ggtccacctg gattgaagta cgacggcgac gcctgctgcc gggcgttgtt
    16741 tgcgtctacg ccggtttcct gcacacgatt gcgagcttgt tgccggtggg gtcgcgcaga
    16801 ctatcaaatc aaaagaacat gccctcgggc gtgggattgg ccaatcatgt ccgtcgcaga
    16861 tccaaaacag gccacccaaa cgcacgacgc cgccaggctg aatgatgctt ggcggcgtcg
    16921 gctaaatccg gacagtgcaa tcccccggta ccgatatgtc tgtacagggc ctgccgaggt
    16981 tcaaacggtt cgaaaaagta tctgc
//
LOCUS       DATNOI010000333         3000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2319593,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000333 DATNOI010000000
VERSION     DATNOI010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2319593"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VN968_13225"
     CDS             complement(<1..579)
                     /locus_tag="VN968_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL30236.1"
                     /translation="MNEISVSMSAAGGPVPPVTLPEAPAKIAARSLNFYYGENLALKN
                     INLTLGTHRVTAFIGPSGCGKSTLLRTFNRMYDLYPGQRATGQLMLDQTNILDPKLDL
                     NLLRARVGMVFQKPTPFPMTIYENIAFGIRLYEKISKSEMDGRVEKALRGGALWNEVK
                     DKLNASGLSLSGGQQQRLCIARTVAVRPEVILF"
     gene            complement(576..1421)
                     /gene="pstA"
                     /locus_tag="VN968_13230"
     CDS             complement(576..1421)
                     /gene="pstA"
                     /locus_tag="VN968_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HXL30237.1"
                     /translation="MNPIYVSRRRSDIVVKFLCLGAALFGVTWLFLILFTLFYNGLAG
                     LNLQVFTQNTPPPGSTEGGLLNAITGSIIMTAIGVGIGAPLGLLAGTYLAEYGRNDRL
                     TPVIRFINDILLSAPSIIIGLFIYGAIVVPMGGFSAFAGSLALAVIVIPVVVRTTEDM
                     LLLVPNPLREAASALGLPRSLVIKRIAYRAARAGLITGVLLATARVAGETAPLLFTAL
                     SNQFFSLDLTKTMANLPVTINNFVQSPYAYWKQLAWSGALLITLTVLALNIGARILGA
                     ERTPK"
     gene            complement(1426..2415)
                     /gene="pstC"
                     /locus_tag="VN968_13235"
     CDS             complement(1426..2415)
                     /gene="pstC"
                     /locus_tag="VN968_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HXL30238.1"
                     /translation="MAVQSDVMEAAGPYDRARALNAFKLGDVTFYWITRACAISVLLI
                     LGGIILSLIVGAWPAMKTYGLSFLFASRWAPSADPPVLGALGPIYGTLVTSVIAMMIA
                     IPVGLGIAMFLTELCPQWLRRPIGIAVELLAGIPSIIYGMWGFFVLGPFMADTFQPFM
                     IRLFDGVPVLGSIFAGPPSYLSLFNAALILAIMVLPFITAISVDVFKTVPPVLKEAAY
                     GVGCTTWEVVRNVVIPYTRVGVIGGIMLALGRALGETMAVTFIIGNSFRISSSVFAPG
                     TTISAAIASEFAESDGLHQSGLILLGLLLFVLTFFVLAAARLMLMRLEKKAGG"
     gene            complement(2688..>3000)
                     /locus_tag="VN968_13240"
     CDS             complement(2688..>3000)
                     /locus_tag="VN968_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HXL30239.1"
                     /translation="GKTVQPTVAAFQAAAANADWVGSKNYYVILTDQPGEASWPITGA
                     TFILMHKEPVDKAASAEAIKFFKWAFAKGDKMAEELDYIPMPDSVVKLIEKTWSADIK
                     S"
BASE COUNT          641 a          902 c          939 g          518 t
ORIGIN      
        1 gaacaggatc acctcgggcc gcaccgccac ggtgcgggcg atgcaaagcc gctgctgctg
       61 gccgccggac aggctgaggc cgctggcgtt cagcttgtcc ttgacctcgt tccatagcgc
      121 gccgccgcgc agcgcctttt ctacacggcc gtccatctcg gatttcgaaa tcttctcgta
      181 gagccggatg ccgaacgcga tgttctcata gatcgtcatc ggaaacggcg tcggcttctg
      241 gaacaccata ccgacgcgcg cgcgcagcag gttgaggtcg agcttgggat cgaggatgtt
      301 ggtttgatcg agcatcagct gaccggtggc gcgttgcccc ggatagaggt cgtacatccg
      361 gttgaacgtc cgcagcaacg tcgatttgcc gcatcccgac ggtccaatga aggccgtgac
      421 gcgatgagtg ccgagcgtga ggttgatgtt cttcagtgca aggttctcgc cgtaatagaa
      481 attcaggctg cgcgcagcga tctttgccgg tgcctcgggc aatgtcaccg ggggaacggg
      541 tccgcctgcg gcactcatcg atacagaaat ctcgttcatt ttggtgtcct ctcggcgcca
      601 agaatgcgcg caccaatgtt caaggcgagc accgtcaagg tgatgagcaa ggccccgctc
      661 caggccagct gtttccagta agcgtatggg ctttgcacga aattgttgat ggtcaccggc
      721 aggttggcca tcgtcttggt cagatcgaga ctgaagaact gattggagag cgccgtaaac
      781 aacagcggtg cggtttcgcc ggcaacgcgt gccgtcgcca gcagcacgcc ggtaatcagg
      841 ccggcgcgcg cggcgcggta ggcaatgcgc ttgatgacga gcgagcgcgg cagccccaag
      901 gcagacgccg cctcgcgcag cggattgggc accagcagca gcatgtcttc ggtggtgcgc
      961 acgacgacgg gaatgacgat caccgccagc gccagagacc cggcgaaggc ggaaaacccg
     1021 cccatcggaa ccacgatggc gccgtagatg aacaggccga tgatgatcga gggcgcgctc
     1081 aggagaatgt cgttgataaa gcggatcacc ggggtgaggc ggtcgttcct gccatattcg
     1141 gcgagatagg tgccggccaa caggccgagc ggcgcgccga tgccgacgcc gatcgcggtc
     1201 attatgatgg agcccgtgat cgcgttcagc aagccgcctt cggtcgatcc cggcggcggg
     1261 gtgttctggg tgaagacctg cagattgagc cctgcgaggc cgttgtagaa cagcgtgaac
     1321 agaatcaaga agagccaggt cacgccgaac agcgcagccc cgaggcacag gaatttaacg
     1381 acgatgtccg agcggcggcg ggatacatag atcgggttca tgatctcagc cccccgcctt
     1441 cttttccagg cgcatcagca tcagccgcgc ggccgccaaa acgaagaagg tcagcacgaa
     1501 cagcaacagg ccgagcagga tcaggccgga ctgatgcagc ccatcgctct cggcaaattc
     1561 ggatgcgatc gccgctgaaa tggtggttcc cggcgcaaac acggacgagg agatgcggaa
     1621 cgagttgccg atgatgaagg tcaccgccat ggtttcgccg agcgcccggc cgagcgccag
     1681 cataatgccg ccgatcacgc cgacgcgggt atagggaatt accacgttgc gaacgacttc
     1741 ccaggtcgtg cagccgacgc cgtaagctgc ttccttcagc accgggggca ccgtcttgaa
     1801 aacatcgacc gaaatcgcgg tgatgaacgg tagcaccatg atcgcaagga tcagtgccgc
     1861 gttgaacaag ctgaggtagg agggcggtcc tgcgaaaatc gatccgagaa cggggacacc
     1921 gtcgaacagt ctgatcatga acggctgaaa cgtgtcggcc atgaacgggc caagcacgaa
     1981 gaacccccac atcccataga tgatcgacgg gatacccgcg agcagctcca ccgcaatgcc
     2041 gatcggccgg cgcagccatt gcggacagag ctcggtcagg aacatcgcga taccgagccc
     2101 gaccggaatg gcgatcatca tggcgatcac cgaggtcacc agggttccgt agatgggccc
     2161 gagcgcaccg agcacgggcg ggtcggccga cggcgcccat cgcgatgcga acaagaagga
     2221 taagccgtac gtcttcatcg ccggccatgc acccacgatc agcgagagaa tgatgccgcc
     2281 caggatgagc agcaccgaga tcgcgcaggc acgggtaatc cagtaaaagg taacgtcgcc
     2341 gagcttgaac gcgttgagcg ccctggcgcg atcataaggt ccggcggctt ccattacatc
     2401 gctctgaacg gccatgtctg ccacgccaaa tcccctgtct acttcaaagc tacggagaag
     2461 tcgctacgcg cctggagaaa ctcactgcca agctaacccc gtcattgcgg gacgcagcac
     2521 cgaacccggg tttacccgag ttcggcaata tcattgccca agtccgcaac tgccgacctg
     2581 gaagcgcaga cccgaaatcc atactcccga tggcggttat ggattccgcg ggctcgctcg
     2641 ttccgctcgc gccccgaact gacgggagtg cgattttgtg ggcgcgatca gctcttgata
     2701 tccgccgacc aggtcttttc gatcagcttg accaccgaat ccggcatcgg aatgtagtcg
     2761 agttcctcgg ccatcttgtc gcccttggcg aaagcccatt tgaagaactt gatggcttcg
     2821 gcggacgcgg ctttatcaac cggctccttg tgcatcagga tgaaggtcgc gccggtgatc
     2881 ggccacgagg cttcaccggg ctggtcagtc aggatcacgt agtaattctt ggagccaacc
     2941 cagtcggcat tggcagccgc ggcctggaac gccgcaacgg tcggctgcac ggtcttgccg
//
LOCUS       DATNOI010000334        15360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2325517,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000334 DATNOI010000000
VERSION     DATNOI010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15360
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2325517"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1751)
                     /locus_tag="VN968_13245"
     CDS             complement(<1..1751)
                     /locus_tag="VN968_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30240.1"
                     /translation="MATTVFNKTALANGTSTLNVRADALNQSSDLVQAGVVFNDAIRA
                     SLGLFNLSGSGNANPFLGSYTTDIHAVQNDIAAMLATPAGVTLGGQAFTLNTTDTAVL
                     TNIQGQLGTLLAAAPQTTNAATLTAADQTLHAVQNEILQEINNDPHIAAALNNVTFMA
                     NTGANDVAFQNTPAGADDPAALAAATAGNSLKAVGEVFNAAATVAAGGINTANLGEIT
                     NDFTAVQQGLTKILGNATMLAQIEAGETANAAALTTVHLQTVLNQINLQLNKYDNAET
                     TGSATALRGTADNVLDIIDIVQNDANLNTAAGGNGAAGHAGGFAEMPGGLIGTVTKFQ
                     DNQAQTNFWAAFLAEANTINAHLTAIAGGGEQASAALVAQIQNYQNFGATFDAAQGAV
                     FQGRFDNELNNGTLEADTAASVKGLTGILNGDTGAALAADKAMIIAAGQGFAADAMDV
                     SGNNIAIGGATYVGTATTVATATSVNGLAQGTIPVTATPNIANGTGGTVGSGTTGGGG
                     GGGGGAGGGGATTLFNKTALANGTSNLIVRAHALNHSTDLVQVGVVFNDAVRLSTGLF
                     NLSGSGNSIPFLGEYTA"
     gene            2504..3541
                     /gene="cysD"
                     /locus_tag="VN968_13250"
     CDS             2504..3541
                     /gene="cysD"
                     /locus_tag="VN968_13250"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF009214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HXL30241.1"
                     /translation="MPRVEVAPRTWAKEPAVAAPARGADEIQLASAIRRYAAKASAAT
                     RRQLTPHLRRLEAESIEILREVAAEFRKPVMLYSIGKDSGVMLHLARKAFFPARIPFP
                     LLHVDTTWKFREMIEFRDLMARELGLDLLLHVNREGLARGIDPIASGPALHTQVMKTE
                     ALKQALDAHGFDAALGGARRDEEGSRAKERIFSFRSVNHVWDPRRQRPELWNLFNTRI
                     HDGESVRVFPLSNWTETDVWSYIAAEDIPVLPLYFAKPRPVVRRSGALIVVDDKRLPL
                     ELGEIPEMLTVRFRTLGCYPLTGAIESDAADVDDIVVEMQAATRSERQGRLIDHDDAA
                     SMERKKREGYF"
     gene            3541..5436
                     /gene="cysN"
                     /locus_tag="VN968_13255"
     CDS             3541..5436
                     /gene="cysN"
                     /locus_tag="VN968_13255"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HXL30242.1"
                     /translation="MPTAILDAAVGSPALAGTAEKSLLRFLTCGSVDDGKSTLIGRLL
                     YDSKLVLDDQLAAVARASGRYGTLGEETDLALLVDGLEAEREQGITIDVAYRYFATPR
                     RAFIVADTPGHEQYTRNMATGASTADLAVVLVDARKGLLTQSRRHSYICALLGIRSVV
                     LAVNKIDLVAWSEQVFDGIVGEYLDFVRPLNFSSVTPIPMSARYGDNIASRSERTPWY
                     DGPSLLDHLETAEVAQTAARLPLRFPVQWVNRPNLDFRGYAGTLASGRVAVGDNVVVA
                     SSGKVSRVERIVTFNGDRDSATAGDAVTLTLEDEIDIGRGDVLVPPQSRPEVADQFGA
                     HLLWMNEEPLLPGRSYLLRIGTKTLPAQVTMLKHKIDINTLEQRPGRTLGLNEIGFCN
                     LSTASPVAFDAYADNRRTGAFILVDRFTHATAGAGMIAFGLRRATNIHRQSHLVDKTA
                     RAALKHQTPCVLWFTGLSGSGKSMIANLVEKALHERGIHTMMLDGDNIRHGVNRDLGF
                     TEVDRVENIRRVGETAKLFVEAGLVVLSCFISPFRAERDMVRELLAPGEFAEIFVDVP
                     LEECIRRDPKGLYAKALAGEITNFTGIDSPYESPRAPDIHVAGVEETSEVAAARIVDW
                     FFARQGR"
     gene            complement(5541..8411)
                     /locus_tag="VN968_13260"
     CDS             complement(5541..8411)
                     /locus_tag="VN968_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXL30243.1"
                     /translation="MMTPTAPAREVISFGPFSLITSERLLRREGAPVELSARALDILI
                     ALLSRPNEVVSKNDLLARVWPDATVEEGSLRFHIVNLRKALGDGKDGARYITTLTGRG
                     YCFVAPISRSSDQDQILQGKAPTTAAVSFPHANLPGRLIGMVGRDDDVLKLSARLNAA
                     RFVTIVGSGGVGKTTVAVAVGYHLIEAFAGAVLFVDLSMLNNPGLVTTAVASMLGLSV
                     QSIDATPSLIAYLRDKRILLILDTCEHLIEEVATLASHLHMAAPQVYILATSRETLQA
                     DGEHVYRLDPLACPPDGTGITAAVAQTFPAPKLFVERAVASGAHLDFSDAEAAIVVSI
                     CRKLDGVALAIELAARRVKAYGLHQTAALLDQRLTLLWVGPRTAPPRQKTLHATLDWS
                     YGLLSETERQVLRRLAIFVGHFTLDAALAVVTSATLDQAGVFGAIDSLVAKSMVATRP
                     VGAMMRYRLLDTTRAYALEMSIEDTDLADLAVRHATYYRRWLEQAGTEWSTLSTGVEQ
                     APHFAGLNNVRAALEWCFGVNDNAEIGVGLAAAAAPVFLTMSLLPECHRWSDRAILVL
                     DDATRGGSEEMHLQACLGISSMHMLGQSDAARMALDRSFALAEEHDDVLSQAGLLGML
                     HMFHFRGGDFNTALHHAKRCRAVAGAIEDPAATALAHSILGRSLHLMGDHSGARGELE
                     ASLRNWLRTRRTTIYLAYDRHYRTGVALARTLWLQGYPAQAVERAHEAIRGAERTDHP
                     ASLVVVLAWAASVFLWTGDLRSAEEHINACISLAESYSLGPLKAVGRGRQGELAIRQG
                     DASDGFEGLRASLAKIHAARYELLTTEFNISLAQGLATIRRPAEALTLINETIQRVET
                     NGDVLYVPELLRVKGGLLLSMPRPSVEDAEKCFMQSLELSRRQGARAWELRTAVDLAT
                     LLADQGRRENGRALLQPVFEQFVEGWDTADLKAAERVLSTLS"
     gene            8710..10038
                     /locus_tag="VN968_13265"
     CDS             8710..10038
                     /locus_tag="VN968_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HXL30244.1"
                     /translation="MRTTAITEAIDQDRRRLLGTAAIGIAVAGAVSLLPAHPASATEG
                     DAIRPFHISVPEEALVDLRGRIAATRWPERETVTDQSQGVQLATIQKLARYWQTDHDW
                     RKIEAKLNALPQFVTEIDGLDIHFIHVRSKHENALPLIVTHGWPGSIIEQLKIVDPLT
                     NPTAHGASASDAFHLVIPSLPGHGFSGKPTAPGWTPASIARAWATLMQRLGYTRYVAQ
                     GGDWGNAVSEVMALQQPPGLLGIHTNMAATVPADVSKALSVGGPPPAGLSADEKHAWD
                     QLDDFYKHGLAYAQEMSNRPQTLYGIADSPVGLAAWMLDHDIRSYGMISRVFDGKTEG
                     LTRDDVLDNVTLYWLTNTAISSARLYWDTAQISTGGGFFDVRGVTIPVAVSAFPDEIY
                     AAPRSWAEQAYPKLIHYNKLDKGGHFAAWEQPDLFTAEMRAAFKSLRQSI"
     gene            10115..11182
                     /locus_tag="VN968_13270"
     CDS             10115..11182
                     /locus_tag="VN968_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL30245.1"
                     /translation="MDKIKTSETIDRQRRRFFGAAALTLAAAEFGMIGSADAQAGKAK
                     STGLPATKPGTHTSFASLKQIDAGVLNVGYAEAGPADGSPVILLHGWPYDIYSFVDVT
                     PLLASAGYRVIVPYLRGYGTTRFLSNETVRNGQPSATAVDIIALMDALKVEKATLAGF
                     DWGARTANIVAALWPGRCKATVSVSGYLIGSPEAGRMPLPPKAELQWWYQFYFATERG
                     RAGYDKYRHDFAKLIWQLASPKWDFDDATFDRSAAAFDNPDHVAIVIHNYRWRLGLAE
                     GESKYDDLEKRLAATPAITVPTITLEGDANGAPHPEPTSYAKMFSGKYSHRTITGGIG
                     HNLPQEAPQAFAEAVIDVIES"
     gene            11233..11679
                     /locus_tag="VN968_13275"
     CDS             11233..11679
                     /locus_tag="VN968_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HXL30246.1"
                     /translation="MTQLEKEHTTAKEGKVVYTAKTHTSGGRENGISRSSDGRLDVKF
                     SLPGSARIGTNPEQLFAAGWSACFESAIGLAAHRRKIALPADLAIDAEVDLHLAENGY
                     FLSTRLNISLPGIEREVAQSLVDEADQLCPYSKATRGNIDVAINLV"
     gene            complement(11881..12897)
                     /locus_tag="VN968_13280"
     CDS             complement(11881..12897)
                     /locus_tag="VN968_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012753786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HXL30247.1"
                     /translation="MSVASSAVLTTEPVNSEGIVAAGAYVDGRRVADIAIGEASSWRS
                     RPGHVVWIGLHEPDMALLTSVQRQFQLHDLAIEDADHAHQRPKIEQYGDALFIVARTA
                     QLVGDSIAFGETHLFVGEGYLVSVRHGASTSYTPVRERCESCPRALARGEDYILYAVL
                     DFIVDNYSPVLETIQEEVEAMEALVLASAMTRAQIERLYLLRRDLLRLRNAVGPLVEV
                     CRRLEHDNLPMVRPTMQPLFRDVTDHVRTVQEQIDSLREVLAFAFEASLLVGQAQETA
                     VSKKLASWLAIIAVPTAIAGIYGMNFKNMPELQWEYGYVTVMGVILIACAVLYWRFRR
                     VVWL"
     gene            complement(13091..14449)
                     /gene="hflX"
                     /locus_tag="VN968_13285"
     CDS             complement(13091..14449)
                     /gene="hflX"
                     /locus_tag="VN968_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HXL30248.1"
                     /translation="METRPPVPRAILVGIQVPGVNDVAHAADIEELGRLVKTLGYEVV
                     GTISQKRDEIGGGTVLGKGRLEELAAMTGGTGVVGSMAIPRKSKARERFEGAGDNRPN
                     VRRTEPDPDAKAKPEFVIVDHDISPNQARNLERATGAQVLDRTGVIVEIFHRHAHSRE
                     AKLQVEIARLKYVSPRLRESSRGGRQQGPGAGESDLELDRRKVRDRLAELKIQLEDIQ
                     RDNDQRRSARRDQLRVALVGYTNAGKSSLMRALTGSEVLVEDKLFATLDTTVRTLQPE
                     TKPRVLVSDTVGFIKKLPHDLVASFRSTLAEALEASLLLFVVDASDPTHESQLEVSRN
                     VLREIGADVVPSRLILNKIDRVSEADRAALRAKHPDAILLSAKSPEDVAALRKTMIAF
                     FEAAMVEDQLVLPYAKQGLLSDVYENARVLSEDYDTTGRIIKVRGLPGAIARLRRTLA
                     TP"
     gene            14884..>15360
                     /locus_tag="VN968_13290"
     CDS             14884..>15360
                     /locus_tag="VN968_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30249.1"
                     /translation="MSSKPFAAALLFAGLGLSNTAFAEEDRKPPKPPIILESTGAYEV
                     GGKVITKPGDPSQTLSCDHGYVEYFIPVKRRSVGLIMWHSSSTKVWENRWDGGEGYKS
                     IFLRKGYPVYLWDGPRVGRANWSCEPITYTPAYFDQRNFAAWRFGLTYPNWHPGLQF"
BASE COUNT         2843 a         4854 c         4723 g         2940 t
ORIGIN      
        1 tcggccgtgt attctcccag gaaggggatc gagttgccgc tgcccgacag attgaacagg
       61 cctgtcgata aacggaccgc atcgttgaag acgacgccga cctgcaccag gtctgtcgag
      121 tgattgagcg cgtgagcgcg cacgatcaaa ttcgacgtgc cgttcgccag cgccgtcttg
      181 ttgaacaacg tcgttgcccc gccgccacca gcaccaccac cgcctccgcc gccaccaccc
      241 gtcgtgccag aacccaccgt accgccggtg ccattggcga tattcggggt cgccgtcacc
      301 gggatggtgc cctgtgccag accattgacc gaggtcgccg tcgccaccgt cgtcgccgta
      361 ccgacatagg tggctccgcc gatggcaatg ttgttaccgc tgacgtccat agcgtcggcg
      421 gcaaagccct ggccggcagc gatgatcatc gccttgtccg ccgcgagcgc cgcgccggta
      481 tcgccgttca ggatgccggt gagccccttg accgaggcgg cggtatcggc ctccagcgtg
      541 ccgttgttca gttcgttgtc gaaccggccc tggaagaccg caccttgcgc agcatcgaaa
      601 gtggcgccga aattttggta gttctggatc tgcgcgacca gcgccgcgct tgcctgctcc
      661 ccaccgccgg cgatcgccgt cagatgcgcg ttgatggtgt tggcctcggc caggaacgcg
      721 gcccagaaat tggtctgcgc ctgattatcc tggaacttgg tgacggtccc gatcagaccg
      781 cccggcattt cagcgaagcc gccagcatgc cccgcggcgc cgttgccgcc cgccgccgtg
      841 ttcaggttgg cgtcattctg aacgatatcg atgatgtcga gcacattgtc cgccgtgccc
      901 cgcagcgcgg tggccgagcc ggtcgtctct gcgttgtcat atttgttgag ctggaggttg
      961 atctggttca gcaccgtctg gaggtgaacg gtcgtcagcg ccgcggcatt ggcggtttcg
     1021 cccgcttcga tctgcgccag catggtcgca ttgccgagga tcttggtcag accctgctga
     1081 accgcggtga agtcgttcgt gatctcgccg aggttcgccg tattgatgcc gccggctgcg
     1141 acagtggccg ccgcattgaa gacctcgccg accgctttca ggctgttgcc cgcggtcgcc
     1201 gcagcgagcg ctgccggatc atcggcgccc gccggagtgt tttggaacgc aacgtcgttg
     1261 gcgcccgtat tggccatgaa cgtgacgttg ttgagcgcgg ctgcgatatg gggatcgttg
     1321 ttgatttcct gcaggatctc gttttgcacc gcgtggagcg tctggtccgc agccgtcagc
     1381 gtcgccgcat tggtggtctg tggcgccgcc gccaaaagcg tgcccagttg tccctggatg
     1441 ttggtcaaaa ccgcggtgtc ggtggtgttg agggtaaagg cctgtccgcc gagcgtcacg
     1501 ccagcgggcg tggcgagcat cgcggcgatg tcgttctgga ccgcatggat atcggtggtg
     1561 taactaccca ggaagggatt cgcgttgccg ctgcccgaca gattgaacag gcccagcgac
     1621 gcacggatgg catcgttaaa gacaacgccg gcctgcacca ggtcgctcga ttgattgagc
     1681 gcgtcggcgc gcacattcaa agtcgacgtg ccattcgcca gcgcagtctt gttgaacact
     1741 gtagttgcca ttagatcgtc tccccggatt tcatcgccca tctcctgccg gcgcgtccaa
     1801 caggatcaag aggatgttca aaactgaaga gccccttcac cgacgttcgc cggcttgctg
     1861 agctggtgag cggatgtcat gcgccgcgct accgattgat cgagggcaaa cttgacctaa
     1921 acgtgcgtcg tttcggacgg aactgtgatt atgttctgat caaacgaggc gatcaggtgg
     1981 cgcaaactgc ggcatgaatc ggctgccacc gtcgcctggg tgatccgaat tgaccaatgg
     2041 aaaactctga atgcgtcatc acgcctgcgc gctccatgtg cacttctatg tgcactgagg
     2101 cgtcgacaag cccctagcga cactcgcagt ccggcgcatg ccgcggtttg cgcgacctga
     2161 tcgcctcgtt tggtcagaac atgatcacag ttccgtccga aacgacgcat gcttaggtca
     2221 aatttgaccg cgatcacgcc cgttgcgatc agtcgatacc tctctgtcgt gttgcggctt
     2281 gctgtttgcg gacgcgcagc gccacacttg cgaccttgcg ggactcggct gttattgcag
     2341 cagcaatccg aagctgatgc gccattggtg aagaggtctt gccggaagct gtgatgctga
     2401 acgcgtgcta tgagaacgtc atcgatgata tccagggaca cgcccgtcgg ctcatcgcgc
     2461 atggcgtcgt ccgccgcgtt ttacagggta cacggtcgtg aacatgcctc gcgttgaggt
     2521 ggctcccaga acctgggcga aagaacctgc cgtcgccgcg cccgctcggg gcgcggacga
     2581 aattcagctc gcttccgcca tccgtcgcta tgcggcaaag gcctccgcgg cgacccgtcg
     2641 tcaactcacg ccacatttgc gtcgcctcga ggcagagtcc atcgagatct tgcgcgaggt
     2701 tgcggccgag ttccgcaagc cggtcatgct ctattcgatc ggcaaggatt ccggtgtcat
     2761 gctgcatctg gcacgcaagg ctttcttccc tgccaggatt ccgttcccgc tgctgcatgt
     2821 cgatacgacc tggaaattcc gggagatgat cgaattccgc gacctcatgg cccgtgagct
     2881 tggcctcgat ctgctgctcc acgtcaaccg cgaggggctt gcccgcggca tcgatccgat
     2941 cgcctcgggc ccggcgttgc acacccaggt gatgaagacc gaggcgctca agcaggcgct
     3001 tgatgctcac ggcttcgatg ccgcgcttgg cggcgcgcgg cgcgacgagg agggcagccg
     3061 cgccaaagaa cggatctttt cgttccgcag cgtcaaccat gtctgggatc cgcgccgcca
     3121 gcgcccggag ctgtggaacc tgttcaacac ccgcatccac gacggcgagt cggttcgggt
     3181 gtttccgttg tcgaactgga ccgagacgga cgtgtggtcc tatatcgcgg ccgaggatat
     3241 tccggtgctg ccgctctatt tcgccaagcc gcggccggtg gtgcgccgat ccggcgcctt
     3301 gatcgtggtc gacgataagc gtctgccgct cgagcttggc gaaataccgg agatgctcac
     3361 cgttcgcttc cgcacgctcg gctgctatcc gctcaccggc gccatcgagt ccgatgccgc
     3421 cgacgtcgac gacatcgtcg tcgaaatgca ggccgctacc aggtccgagc gccagggcag
     3481 gctgatcgac cacgacgacg ccgcgtccat ggagcgcaag aagcgcgaag ggtatttctg
     3541 atgcccacgg cgatcctgga tgccgcagtc ggttcgccag ctctggcggg gacggcggag
     3601 aagagcctgc tgcgtttttt aacctgcggc tcggtcgacg acggcaagtc gaccctgatc
     3661 ggccggctgc tgtacgattc caagctggtg ttggacgatc aactcgcggc cgtcgcgcgc
     3721 gccagcggcc gctacggcac gcttggcgag gagacggatc tcgcgctgct ggtcgatggc
     3781 ctcgaagccg aacgtgagca gggcatcacc atcgacgtcg cctaccgcta tttcgccacg
     3841 ccgcggcgcg cctttatcgt cgccgatacg ccgggccatg aacagtatac ccgcaatatg
     3901 gcgaccggcg cctcgaccgc cgatctggcg gtggtgttgg tcgacgcccg caaaggcctg
     3961 ctcacccaga gccggcgtca cagctacatc tgcgcgttgc ttggcatccg ctcggttgtg
     4021 ctcgcggtca acaagatcga tctggtcgcg tggtccgagc aagtcttcga tggcatcgtc
     4081 ggggaatacc tcgacttcgt caggccgctg aatttcagct ccgtcacacc aatcccaatg
     4141 tctgcgcgtt acggcgacaa tatcgcgtca aggtccgagc gcacgccatg gtacgatggt
     4201 ccctcgctgc tcgatcatct cgagacagcc gaggtcgcac agaccgccgc acggctcccg
     4261 ctgcgtttcc cggtgcaatg ggtgaaccgt ccgaacctcg attttcgcgg ctacgccggc
     4321 acgttggcat cgggccgcgt cgccgtcggc gataacgtcg tcgtcgcatc ctcgggcaag
     4381 gtcagccgcg ttgagcgcat cgtcaccttc aatggcgacc gcgacagcgc gaccgccggc
     4441 gatgcggtca cgcttacgct cgaagacgag atcgacatcg gtcgcggcga tgtcctcgtc
     4501 ccgccgcaga gcaggccgga ggtcgccgac cagtttggtg cgcatctttt gtggatgaac
     4561 gaggaacccc tgctgccggg ccggtcgtat ttgctgcgca tcggcaccaa gacgctgcca
     4621 gcccaggtta ccatgctcaa gcacaagatc gatatcaata cgctggagca aaggcccggc
     4681 cgcacacttg gcctcaacga aatcggcttc tgcaacctgt cgaccgcgag cccggtcgcg
     4741 ttcgacgcct atgcggataa ccgccggacc ggcgccttca ttctcgtcga ccgtttcacc
     4801 catgccaccg ccggcgccgg catgattgcg ttcgggcttc gccgggcgac caacatccat
     4861 cgccagagcc acctcgtcga caaaacggcg cgggcggcgc tcaagcacca gacgccctgc
     4921 gtgctgtggt tcactgggct ctccggctcc ggaaagtcca tgatcgccaa tctggtcgag
     4981 aaagcgctgc acgagcgtgg catccatacc atgatgctcg acggcgacaa tatccgccac
     5041 ggcgtcaatc gcgatctcgg ctttaccgag gtggaccgcg tggaaaacat ccgccgcgtc
     5101 ggtgagaccg ccaagctgtt cgtcgaagcg ggtcttgtgg tgctgagttg cttcatttcg
     5161 ccgttccgcg ccgaacgcga catggtgcgg gagctgctcg cgccgggcga attcgcggag
     5221 atctttgtcg atgtaccgct cgaggagtgt atccgccgcg atccaaaggg cctctatgcc
     5281 aaggccttgg ctggcgagat cacgaatttc accggcatcg acagcccgta tgagtcgcct
     5341 cgggcgcctg atatccacgt cgcgggagtg gaggagacat cggaagtggc cgctgcgcgc
     5401 atcgtcgatt ggttcttcgc ccggcagggg cgatagtctc accgctgttc aagccattac
     5461 caagaaatca acaatcatcg ccgcggtttg gttcattcgg ttgtgcagtg gcggcccagt
     5521 ccggaagggt gagcgaacca ttagctcaag gtcgaaagca cgcgctcggc ggctttcaga
     5581 tctgccgtgt cccagccctc cacgaactgc tcaaacaccg gctgcagaag cgcccggccg
     5641 ttctcgcgtc gtccctgatc ggcaagcaga gttgccagat cgaccgcggt gcgcaactcc
     5701 catgcgcgcg cgccctgacg tcggctcaat tcgagcgact gcataaagca cttttcggca
     5761 tcttcaacgc tgggccgcgg cattgacaga agaaggcctc ccttcacgcg cagtaactct
     5821 ggcacataca agacatctcc gttggtctcc acccgttgga tcgtctcgtt gatcagcgtg
     5881 agggcttcgg cgggccgtcg gatcgttgca agtccttggg cgagcgagat gttgaactcc
     5941 gtggtgagca actcgtaacg cgccgcgtgg atcttcgcga gagacgctcg caagccctcg
     6001 aagccgtcgc ttgcatcgcc ctggcggatg gccagttccc cctggcggcc tcgtcccacc
     6061 gccttgaggg gtcccaggga ataggattcg gcgaggctga tacacgcatt gatatgctct
     6121 tcggcgcttc gaagatcgcc ggtccagagg aataccgacg ctgcccaagc caaaacaacg
     6181 actagcgatg ccgggtggtc ggtacgttcg gcacccctga tggcctcgtg cgcacgttcc
     6241 accgcctgag ccggatagcc ttgcaaccac aatgtccgcg ccagggcgac gcccgtccgg
     6301 tagtggcgat cgtaggcaag atagatcgtg gtccgccggg tgcgcaacca attccgtaac
     6361 gaagcctcaa gctccccgcg ggcgccgcta tggtcgccca taaggtggag cgaccttccc
     6421 aaaatggaat gggctaatgc ggtggcggca ggatcctcaa tggcccccgc aacggctcgg
     6481 cagcgcttgg cgtgatgcag ggcggtgttg aaatccccgc cgcggaaatg gaacatgtgc
     6541 agcatgccca aaagcccggc ttggctgagg acgtcgtcgt gttcttcggc aagcgcaaag
     6601 cttctatcca atgccatgcg cgccgcatcg ctttgtccga gcatgtgcat cgaggagatt
     6661 cccagacacg cctgaagatg catctcctcg gacccgccgc gagttgcatc gtcgaggacg
     6721 agaatggctc ggtccgacca gcgatgacat tcagggagca acgacatcgt caaaaaaacc
     6781 ggagcggcgg cggcggcaag tccaacgccg atttcggcgt tgtcgttgac gccaaagcac
     6841 cattccaggg ccgcgcggac gttgttgaga ccggcaaagt ggggtgcctg ttccacgccg
     6901 gtcgacaagg tcgaccattc ggtcccggcc tgctccagcc atcgccggta ataagtcgcg
     6961 tggcgcacgg ccaagtcagc gaggtcggta tcctcaatac tcatttcgag ggcgtaagcg
     7021 cgagtcgtgt ccaacagccg gtagcgcatc atggccccta cggggcgggt cgcgaccatc
     7081 gacttggcga cgaggctgtc gatggcgccg aaaacacccg cttgatcgag ggtcgcgctg
     7141 gtcacgaccg cgagcgccgc atcgagcgta aaatgtccaa cgaatatggc gagccggcgc
     7201 agtacctggc gttctgtttc ggaaagcagc ccgtagctcc agtccagcgt ggcatgcagc
     7261 gtcttttgac gcggcggcgc ggtgcgcggc cccacccata gcagcgtcag tcgttggtcg
     7321 agaagggcgg cagtctgatg cagaccgtag gctttgaccc gtctcgcggc caactcaatc
     7381 gccagcgcca caccatcgag ctttcggcag atgcttacca cgattgcggc ttccgcatcg
     7441 ctgaagtcga ggtgcgcacc gctcgccacc gcgcgttcga cgaacagctt tggtgcgggg
     7501 aacgtctgag caaccgccgc cgtgatcccc gttccgtccg gcggacaggc aagcgggtcc
     7561 agtctgtaga cgtgctctcc atcggcctgg agggtctcgc gacttgtcgc caggatgtag
     7621 acctgcggtg cggccatgtg gagatgcgac gccagagttg ccacttcctc aatgaggtgc
     7681 tcgcaagtat cgaggatcag aagaattcgc ttgtcccgca gataggcgat caaactgggt
     7741 gttgcatcga tagactgaac cgatagcccc agcatggagg caacggccgt ggtgacgagg
     7801 ccgggattgt tcagcatgct gagatcgacg aagagcacgg cgccggcgaa ggcctcgatg
     7861 aggtgatatc cgaccgcgac cgcaaccgtg gtcttgccaa cgccaccgga gccgacgatt
     7921 gtgacaaagc gcgccgcgtt cagccgagcc gacagcttga gaacgtcatc gtcgcgtccg
     7981 accattccga tcaggcggcc gggcaggttg gcgtgcggaa aactgacggc cgccgtcgtc
     8041 ggcgctttac cttgaagaat ctggtcctga tcgctcgatc gagaaatcgg ggccacgaag
     8101 cagtagcctc gcccggtcag ggtcgtgatg tatcgcgcgc cgtccttgcc atcgcccagc
     8161 gccttgcgca ggttgacgat gtggaaccgc aaactgcctt cttccacggt ggcgtcgggc
     8221 cagacccgag ccaatagatc gttcttgctg acgacctcgt taggacggga caacagggca
     8281 atcaaaatgt cgagcgctcg cgcgctcaat tccacgggcg cgccctctct tctgaggagt
     8341 ctttcgctcg tgatgagact gaacgggccg aatgatatga cctctctggc cggcgccgtc
     8401 ggtgtcatca tggcgtcatc ttcgtagccg tcagccccgg ggctgcattc taggtcaatg
     8461 tctggttccc tactatagga ataccacgaa gacgaccggc ccgggttccc gagaaagtgg
     8521 caagaccatg ccttgcgaat tgccgctgct cgcaatcgct aacagcgcct aacaagctat
     8581 agcaggcaat ttcccggcca ctgcgtcaat gtttggggca acgaccgcat ccggcaaaat
     8641 gcctcggatg ccttcgaacg aaccgcaatg tgctgacccg atcgatcatc aaaccaggga
     8701 gattcaccga tgcgcaccac agcaatcacc gaagcaatcg accaggatcg ccgccggctt
     8761 ttgggcaccg ccgcgatcgg catcgccgtc gccggtgccg tgagcttgct tccggcccat
     8821 ccggcctctg cgaccgaagg tgacgccatc cgtcccttcc atatcagcgt tccggaggaa
     8881 gcgctcgtcg atctccgcgg acgcatcgcg gcgacccgct ggcctgaacg ggaaacggtc
     8941 acggatcaat cgcagggcgt ccagctcgcg acgattcaga aactcgcgcg ctattggcag
     9001 acggaccatg attggcggaa gatcgaggcc aagctgaacg ccttgccgca attcgtgacc
     9061 gagatcgacg ggcttgacat tcatttcatc cacgttcgct cgaaacatga aaatgcgttg
     9121 ccgctcatcg tcacgcacgg gtggcccggc tcgatcatcg agcagctgaa gattgtcgat
     9181 ccactgacca atcccacggc acatggcgcg agcgcatcgg acgctttcca tctggtgatc
     9241 ccgtcgctgc ctggacacgg cttttcgggc aagccgaccg cgccggggtg gacccccgcc
     9301 agtattgctc gtgcgtgggc gacgttgatg caacgcctcg gctacacacg gtacgtggcg
     9361 cagggcgggg attgggggaa tgccgtctcg gaggtgatgg cgctgcagca gccgccggga
     9421 ttgctcggca ttcacaccaa tatggcggcc acggttccag ccgatgtttc aaaagctctt
     9481 tcggttggcg gcccgccgcc cgccggcctc tcagccgatg aaaaacatgc gtgggaccag
     9541 ctcgatgatt tctacaagca cggcctggcc tacgcccagg aaatgtcgaa ccggccacag
     9601 acgctttacg gaatcgcgga ttcgccggtc ggcctcgcgg cgtggatgct ggaccacgac
     9661 atccgcagtt acggcatgat ctcacgcgtc ttcgatggaa agacggaggg gctgactcgg
     9721 gacgacgtcc tcgacaacgt cacgctctac tggttgacga acaccgcgat ttcctcggcg
     9781 cgcctctact gggacaccgc gcagatttcg acgggaggag gtttcttcga cgtcaggggc
     9841 gtcacgatcc cggtcgcggt gagcgccttc cccgatgaga tctatgcagc tccgcggagt
     9901 tgggcagagc aggcgtatcc caagctcatc cattacaaca agctcgacaa aggcggtcac
     9961 ttcgccgcct gggaacagcc ggatctcttc actgcagaga tgcgggctgc gttcaaatcg
    10021 ctgcgccagt caatctgaga cgaaaagcgg cgctgcagtg cgcgcgccgc ggttccgtaa
    10081 cacgtgacac cctatcgaaa gacggagacc gaccatggat aagatcaaga catccgaaac
    10141 aatcgaccgg cagcggcgcc gtttctttgg cgctgcggcg ctcacccttg ccgccgcaga
    10201 attcggtatg atcggctccg cggatgcaca agcgggcaag gctaaatcga cagggctacc
    10261 ggccaccaag ccaggtacgc acacctcgtt cgcctcgctg aagcagatcg atgccggcgt
    10321 tctcaacgtc ggatacgccg aagcaggtcc ggccgacggc tcgcctgtca tcctcctgca
    10381 cggctggccc tacgatatct acagcttcgt cgatgtgacg ccgttgctgg cgtcggcggg
    10441 ctaccgtgtg atcgtgccgt atctgcgcgg ctatggcacc acgcgctttc tttcaaacga
    10501 aacggtccgg aacggccagc cctcggcgac cgccgtcgat atcattgcgt tgatggatgc
    10561 gctcaaggtc gagaaggcga cgcttgctgg gtttgattgg ggagcgcgta ccgccaacat
    10621 cgttgcagcg ctctggcctg ggcgctgcaa ggccacggtc tcggtgagcg gctacctgat
    10681 cggcagcccg gaagccggca ggatgccgtt gccgccaaag gctgagctgc aatggtggta
    10741 ccagttttac ttcgctaccg aacgcggccg ggccggctac gacaaatacc ggcacgactt
    10801 tgcgaagctg atctggcagc tcgcctctcc gaagtgggac ttcgatgacg ccaccttcga
    10861 tcgcagcgcc gccgccttcg acaatcctga tcatgtcgcg atcgtgatcc acaattaccg
    10921 ctggcggctt ggcctcgccg aaggcgagtc aaaatacgac gatctggaaa agcggcttgc
    10981 cgcgacgccg gccatcactg tgcccaccat taccctcgaa ggcgatgcta acggtgcgcc
    11041 acacccggaa ccgacttcct acgccaagat gttctcgggc aaatattcgc accgaaccat
    11101 cacgggcggc atcggacaca atctgcctca ggaagcccct caggcctttg ccgaagctgt
    11161 catcgacgtc atcgaaagtt gattgatcgt cgcgggcgca gcgggaggca ggaaaagcaa
    11221 caggagagca ccatgaccca actcgaaaaa gaacacacga cggccaagga gggaaaagta
    11281 gtgtacacgg ccaagaccca caccagcggc gggcgggaaa acggcatatc ccgcagctct
    11341 gatggccgcc tcgacgtcaa gttttcactc ccgggctcgg cgcgcatcgg caccaatccg
    11401 gagcagctgt tcgccgcggg ttggtcagcc tgtttcgaga gtgcgattgg gcttgcggcc
    11461 cacaggagga agatcgctct gccggcggac ctggctatcg acgccgaagt ggacctgcat
    11521 ctggcagaaa acggttactt tctcagcacc cgcctcaaca tcagcctgcc tggtatcgag
    11581 cgcgaagttg cccagagcct ggtggatgaa gcggaccagc tttgtccgta ctcaaaggcc
    11641 acgcgcggca acatcgacgt tgcgatcaat ttggtctgag ccgaaccggc tcgcgcgcgc
    11701 cccgaaggct gaacatggcg ggtgtcggtc gtgctaccga tggtcgctgc gtttctcttc
    11761 gcgctctatc tggctttggc cggatgtttt tgtttcaggg tgtttcccgc aggaaaaggc
    11821 ggtggcgtct tgcggggccg atccccgaag cccagcgccg gggatctcgg atctgccggc
    11881 tcacagccat accacgcgtc ggaatcgcca gtacagcacc gcgcatgcga tcaggatcac
    11941 gcccataacg gtgacgtaac catattccca ttgcagttcc ggcatgttct tgaagttcat
    12001 gccgtagatg ccggcgatcg cggtcgggac cgcgatgatg gcaagccatg acgccagttt
    12061 tttcgagacc gcggtctcct gggcctgacc gaccagaagg ctcgcctcga aggcaaatgc
    12121 aaggacctct cgaagcgagt cgatctgctc ctgaaccgtc ctgacgtggt ctgtgacatc
    12181 gcggaacagc ggttgcatgg tgggccgcac catcggcagg ttgtcatgct caagcctgcg
    12241 gcagacttcc accaggggcc cgaccgcgtt tcgcaagcgc aacaggtcgc gtcgcagcag
    12301 ataaagccgc tcgatctggg cccgcgtcat cgcgctcgca agcacaagcg cctccatggc
    12361 ctcgacttct tcctggatgg tttcgagcac cggcgagtag ttatcgacga tgaaatcgag
    12421 gacggcgtag aggatataat cctcgccgcg ggccagcgcc cgcgggcagc tttcgcagcg
    12481 ctcgcgtacc ggcgtatagg acgtcgacgc gccatgacgg acggaaacaa gatatccttc
    12541 gccgacgaac agatgggtct cgccaaatgc gatgctgtcg ccaaccagtt gcgcggtgcg
    12601 cgccacgatg aacagcgcat cgccatattg ttcgatcttc ggtcgctggt gggcatggtc
    12661 cgcgtcctcg atggcgagat cgtgcagctg gaattgccgc tgcacgctgg tcaaaagcgc
    12721 catatccggt tcgtgcagtc cgatccacac cacatggcca ggccggctgc gccagctcga
    12781 ggcttcgcct atggcgatat cggcaacgcg ccggccatcg acataggcgc cagcagcgac
    12841 gatgccttcc gaattgacag gctcggtcgt caagacggcc gacgacgcta cgctcatggt
    12901 cttcctcccc agcgattgct gttgaacttc actccggggc aaagcttcgg caaggctagc
    12961 accggaaaac ccgccgtcaa atggaatgtc aggacttcat cggcgaagac ccggcaagct
    13021 tctgtgtcgg cctgacaccg gcgactttgt ctgcctcaat attggcggcc ggccgatcgg
    13081 cgatcgaaac tcatggcgtg gcgagagttc ttcgcaggcg cgcgatcgcg ccgggcaggc
    13141 cgcgcacctt gatgatccgg ccggtggtgt cgtagtcctc ggacagcacg cgtgcattct
    13201 cataaacgtc gctgagcagc ccctgcttcg cgtagggcag caccaattgg tcctctacca
    13261 tcgcagcttc gaaaaacgcg atcatcgtct tgcgtagcgc ggccacgtcc tcgggtgact
    13321 ttgcggagag caggatcgcg tcggggtgct ttgcgcgcag cgcggcgcga tcggcctcgc
    13381 tcacgcgatc gatcttgttg aggatcaggc gcgacgggac gacgtccgcg ccgatctcgc
    13441 gcagcacgtt gcggctcacc tcgagctgcg attcatgggt tggatcggac gcgtcaacga
    13501 cgaacagcag cagcgacgcc tcaagcgctt cggcgagcgt cgaccggaag gacgcgacca
    13561 gatcgtgtgg cagcttcttg atgaacccga ccgtatccga gaccagcacg cgcggcttcg
    13621 tctccggctg cagggtgcgc acggtggtgt cgagcgtggc gaacagtttg tcctctacca
    13681 ggacctcgct gcccgtgagc gcccgcatca gcgacgactt ccccgcattg gtgtagccaa
    13741 ccagtgcgac ccgaagctga tcgcgacgcg cggaccgacg ctggtcgttg tctcgctgga
    13801 tatcctccag ctggattttc agctccgcga ggcgatcacg gaccttgcgg cgatcaagtt
    13861 cgagatcgga ttcgcccgcg ccgggaccct gctgtcgccc gccgcgcgac gattcccgca
    13921 aacgcggcga gacgtatttc agccgggcga tctcgacctg cagcttcgcc tcgcgactgt
    13981 gcgcatgccg atggaagatc tccacgatca ccccggtccg atcgagtacc tgcgcgccgg
    14041 ttgcgcgctc gaggttgcgg gcctggttcg gcgagatgtc atgatcgacg atcacgaatt
    14101 caggcttcgc cttcgcgtcc ggatcaggct ctgtccgcct gacattcggc ctgttgtcgc
    14161 cggcgccttc aaagcgctcg cgggccttcg acttccgagg tatcgccatc gatccgacga
    14221 ctccggtccc gccggtcatc gccgcgagtt cctcaagccg gcctttgccg agcaccgtcc
    14281 cgccgccgat ctcgtcgcgc ttctgcgata tcgtgccgac gacctcgtag ccgagcgtct
    14341 tcaccaagcg gcccaactct tcgatatcgg cggcatgcgc gacgtcgttg acaccgggaa
    14401 cctggatgcc gacgaggatc gcgcggggaa cgggcggtct ggtttccatg atacatcgct
    14461 tgcctgctcg atggcgggct gttacgcggc ctcgcggacc ggattcattt ggcgtcgccg
    14521 cgctgaaacg gcacagccgc attcatggac cgttccggcc tcgatgacaa atccgcgcct
    14581 ttccagggat aggcgaagga tttcgctggc cgtgcccggc gcaaatacga tgctgaatgc
    14641 actgccggca cgcatcgtct caaaatcgct tctcggacag agcgaggtcc tccgcgtttt
    14701 ggttcttggg tcaaatggag ggcggagcaa agattgttgg cggcatggct ggaattccat
    14761 ctggtggaac gcagcggaat agctatggcc gatgagcgtc ctccctgtaa tggtttcgcc
    14821 aagcgctacg tcggagcccg tcttctgagg gcggcccgtg atggccgggg aggaggatgc
    14881 gctatgtcgt cgaagccgtt tgccgcggca ttgttgtttg cgggacttgg actaagcaat
    14941 acggccttcg ctgaagagga tcgcaaacca ccgaagcctc ctatcatcct ggaatcaacg
    15001 ggcgcgtatg aagtgggcgg caaggtcatt accaagcccg gtgatcccag ccagacactc
    15061 tcctgcgacc atggctacgt tgaatacttt atccccgtga aaaggcgcag cgtcggcctg
    15121 atcatgtggc acagttccag tacgaaggtg tgggaaaacc gctgggacgg cggcgaaggt
    15181 tacaagagca ttttcctgcg caagggatat cctgtttacc tgtgggacgg cccgcgcgtc
    15241 ggtcgcgcga actggagttg cgagccgatc acctacaccc cggcgtattt cgatcaacgc
    15301 aactttgccg cctggcgttt cggcctgacc tatcccaact ggcatccggg cctgcagttc
//
LOCUS       DATNOI010000335        14244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1832481,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000335 DATNOI010000000
VERSION     DATNOI010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 14244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14244
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832481"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VN968_13295"
     CDS             <1..491
                     /locus_tag="VN968_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HXL30250.1"
                     /translation="KLTGADVPVCLGSRPCVMTGVGETLLPLSLPRLPCVMVNPRVPV
                     ATRDVFKALGLRNGELLVGVTDVLRATAWPEDGASVEDWVEVLAASSNDLEAPAIRIQ
                     PVIGAVLSALSAANGAWLVRMSGSGATCFAIFENTPEAQRAAQKIQLDHPQWWVHAGT
                     LS"
     gene            complement(488..1138)
                     /locus_tag="VN968_13300"
     CDS             complement(488..1138)
                     /locus_tag="VN968_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HXL30251.1"
                     /translation="MGFAALNPSYDISLFLLAAVVIAAVPGPGIFYVAAKTLSGGRRA
                     GIASTFGTALGGLVHVVAGGIGVSAIILASAQLFTMLKLAGALYLIWLGIRTIREAGH
                     LPTEPTGAIGFRRAFREGIVVEALNPKTAAFFLAFIPQFLEPAAGHAALQFMTLGLIS
                     VTFNTFADVIVIVAASAARAGLVRRPHLVQRLRQGSGLFIAGLGISLALARRPATS"
     gene            complement(1206..2216)
                     /locus_tag="VN968_13305"
     CDS             complement(1206..2216)
                     /locus_tag="VN968_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXL30252.1"
                     /translation="MAVIVPFESPSGASIDQLVGLVAADMERVNATILSRTGSEVIMI
                     PEVANHLISSGGKRLRPMLTLAMAHLTGYSGDGHIKLAASVEFMHTATLLHDDVVDES
                     ELRRGKLSARMLWGNEASVLVGDFLLGQAFRMMVEVGSLRALDILSSAAATIAEGEVM
                     QLAAAKNTATTEDEYLAVIRGKTAELFAAACEVGPVIANRPKAEQTACRSVGMNLGIA
                     FQLVDDVLDYGGKAAKLGKNIGDDFREGKITLPVVLAFRRGNDAERAFWIKALERGEI
                     GDHDLDHAIGLMTKHRALEDTIHRAQHYGAMAVDALALFPASPMKTALEQVVAFCLAR
                     SH"
     gene            2355..2582
                     /locus_tag="VN968_13310"
     CDS             2355..2582
                     /locus_tag="VN968_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HXL30253.1"
                     /translation="MRELVRTNDMVLVSAVGALLDGANIHHLVLDQNMSIIEGSLGVL
                     PRRILVHDDDNREARQILRDAGLAHELRADD"
     gene            2575..3399
                     /locus_tag="VN968_13315"
     CDS             2575..3399
                     /locus_tag="VN968_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXL30254.1"
                     /translation="MTDPAYEFTEDAFLGGRLRLRQPKSGHRAGHDAMLLAAATSARA
                     GDRVVDFGAGVGAAGLALAKRVAGIELVLVEIDEALARLARGNAFSNAIPADVILLDV
                     TSSADRFAAAGLSADSVDVVLMNPPFNDPARHRASPDRAREVAHVATAATLASWVHAA
                     RRILKSGGVLCLIWRADGLAEVLAALDRGFGSLAILPVHGDPKKPAIRVLIRAIKGGK
                     APTEIHAALMLKDESALPNKQVEELLAGKGILPLAMSRKASPAIAEASPSAALALK"
     gene            3566..4477
                     /locus_tag="VN968_13320"
     CDS             3566..4477
                     /locus_tag="VN968_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HXL30255.1"
                     /translation="MMEQTVDRGGLPGLIDRLMEFVPARLRRGVAVVPVVRLSGVIGA
                     VTPLRPGMSLTGVAKTLERAFATKNAKAVALMINSPGGSPVQSRQIYLRIRQLAAEKK
                     LPVLVFVEDVAASGGYMIACAGDEIFCDPSSILGSIGVVGGAFGFQDLIKKIGVERRL
                     YTAGEHKAMLDPFLPENPEDVARVKALQREIHAIFIALVKQSRGGRLKGADHVLFTGE
                     YWAGETSVSLGLADAIGDLRSTLRARYGDKVLTPVIAPATGMLSNLLGRRSAGAGTLT
                     SLDGIAGLPDELISALETRAIWAKFGF"
     gene            4584..4775
                     /locus_tag="VN968_13325"
     CDS             4584..4775
                     /locus_tag="VN968_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30256.1"
                     /translation="MPPLIAFAGALGGLAVVRWAYRTALRVNRELEEARLARLAEADS
                     LGEIPTLRRDPITGAYRPG"
     gene            4891..5826
                     /locus_tag="VN968_13330"
     CDS             4891..5826
                     /locus_tag="VN968_13330"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HXL30257.1"
                     /translation="MDSIPAHMRPERSFQGFILALQRFWADQGCVILQPYDMEMGAGT
                     FHPATTLRALGPRPWKAAYVQPSRRPKDGRYGENPNRLQHYYQFQVIIKPSPPDLQEL
                     YLKSLDAIGIDSSLHDIRFVEDDWESPTLGAWGLGWECWCDGMEVSQFTYFQQVAGVE
                     CAPVAGELTYGLERLAMYVQGVDRVYDLNFNGREGAEKVTYGDVFLQAEQEYSRHNFE
                     YSDADMLFKQFSMAEEACRKYLEAGWKSGSNRTEHLMALPAYDQCIKASHVFNLLDAR
                     GVISVTERQSYIMRVRELAKACGEAWMHTEAGGAT"
     gene            5823..6077
                     /locus_tag="VN968_13335"
     CDS             5823..6077
                     /locus_tag="VN968_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HXL30258.1"
                     /translation="MSEQPAIGGRAPIPVEVEAGKDYWWCACGKSADQPFCDGSHQGS
                     GFSPLQYTAPETKKVFFCTCKRTATMPLCDGSHGRLPTSV"
     gene            6152..8326
                     /gene="glyS"
                     /locus_tag="VN968_13340"
     CDS             6152..8326
                     /gene="glyS"
                     /locus_tag="VN968_13340"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HXL30259.1"
                     /translation="MTDLLLELFSEEIPARMQAKAADDLRRMVTDKLVAEGLVYEGAK
                     AFATPRRLTLTVHGIPARQSDLKEERKGPRVGGPEAAIAGFLKATGLSSLDQAKIQRD
                     PKKGDFYIALIEKPGRATLDVLAEMLPVIIRTFPWPKSMRWGERSARSGALQWVRPLH
                     SIVATFGLETEAPDVVKFDVAGIEAGQTTYGHRFMAPAAISVRRFEDYDAKLLDAKVV
                     LDPQRRKDTILGEAKTLAQAQNYELVEDQALLDEVAGLVEWPVVLMGSFDREFLTIPG
                     EVIRATIRNNQKCFVVSDPKTRKLTNKFILTANIEASDGGKAIIAGNERVIRARLSDA
                     KFFYETDLRTKLEDRLPKFESIVFHEKLGTQGERIARIERLAAEIAPLVGADVEKAKR
                     AARLAKADLLTEVVGEFPELQGLMGKYYALAQGEDASVAAASEEHYKPQGPADRVPTD
                     PVSVAVALADKLYSLVGFWIIDEKPTGSKDPFALRRAALGVIRVICDNNIRLSLSAEL
                     DKATAVFPPNLPNEQSTGAYAAHLISQKPDSGPKREPRYFVVGELLSFFADRLKVQLR
                     EQGARHDLVDAVFALEGQDDLLMVVRRVEALGRFLDTDDGKNLLAGTKRAANILRIEE
                     KKDGKAYDGAPVATLYSLAEEKALAKAIDQVKTEASAAVAKEDFAGAMSVIAKLRPAV
                     DAFFDKVKVNDDDPKVRENRLKLLNEIRAATRAVADFSKIQD"
     gene            9553..10566
                     /locus_tag="VN968_13345"
     CDS             9553..10566
                     /locus_tag="VN968_13345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HXL30260.1"
                     /translation="MSELREAGSNCYKGKNGNRNGAKLVRNKPSKTVSRLLSGQQCAM
                     NDEITDVAGDSKAPDLERTGASPEAIISHYDKGEDFFKLVLGPELIYSCALFESDDDL
                     AEAQRRKLDYHIEAANAANAKRVLDVGCGWGAMLRRLVDHAEVPHVVGLTLSPSQANW
                     IRRQPRPGLEVVEQDWRDHQPTQRYDAIISVGAFEHFVQKGLDPVKKLDAYREFFDFC
                     DRMLVAEGKISLQTIAFSEPHEGHPLLEKTFPDSDLPLIWEPIAAAEGKFVLTTLRND
                     RDHYYRTLRAWEQNLMAHYAEAIELVGESTVADFRRYLRISATGFRMGIVSLLRMSFQ
                     KRM"
     gene            10847..11734
                     /locus_tag="VN968_13350"
     CDS             10847..11734
                     /locus_tag="VN968_13350"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002938.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HXL30261.1"
                     /translation="MSDKISAEDNHARRRVGVHDSEMSYVTVGGGDPIVFLHGNPTSS
                     YLWRNVIPHVSDLGWCLAPDLIGMGQSAVSPSGAYRFLDHARYLDAWFEALDLRRNVT
                     LVLHDWGSALGFHWASRYPERVRAIAYMESMVKPRDWEDLPSSRAPIFRDLRSEKGER
                     MILDENAFIEILLPKLIIRTLTKAEMDAYRLPFREPESRWPTLVWPRELPIGGEPADV
                     VKIFEGYGQWLLRSPLPKLFINAEPGSLLIGRARDFCRSWPNQQESTVRGIHFIQEDS
                     PHEIGSALARFIRQARTAI"
     gene            13477..13638
                     /locus_tag="VN968_13355"
     CDS             13477..13638
                     /locus_tag="VN968_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30262.1"
                     /translation="MHDSGAARREIVKLHLDVIASAAKQSMAATRRKKKDGLLRCARN
                     DGCLWLFEN"
     gene            complement(14008..>14244)
                     /locus_tag="VN968_13360"
     CDS             complement(14008..>14244)
                     /locus_tag="VN968_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HXL30263.1"
                     /translation="VGIAVDQRPAFREYIVRERVPTYTIPDRVIVGGVLPEAGVTYYD
                     VPQAFGPTPYRYTVVNGRTVLVEPRSRRIVQVVE"
BASE COUNT         2798 a         4195 c         4490 g         2761 t
ORIGIN      
        1 cgaaattgac cggcgccgac gtgccggtct gtctgggctc gcgtccctgc gtcatgaccg
       61 gcgtcggtga aaccctgctg ccgctgagct tgccgaggct gccctgcgtg atggtcaatc
      121 cgcgggttcc ggtggcgacc cgcgacgtgt tcaaggcgct gggtttgcgc aatggcgagt
      181 tgctggtcgg cgttaccgac gtgctccggg cgacggcctg gccggaagat ggcgcgtcgg
      241 tcgaagactg ggttgaagtt ctcgcggcga gttccaacga cctcgaagct ccggcaatcc
      301 gcattcagcc tgtcatcggc gcggtgctct cggcgctcag cgccgcaaac ggggcatggc
      361 tggtgcgaat gtcaggatcg ggcgctacct gctttgcaat cttcgagaac acccccgagg
      421 cgcaacgcgc ggcgcagaaa attcagctcg atcacccgca gtggtgggtg catgcgggga
      481 cgctgagcta gcttgtcgcg ggccggcgcg cgagtgcgag cgaaattccg agcccggcga
      541 tgaaaagccc ggagccctgc cgcaggcgct gaaccaaatg tggcctgcgc acgagacctg
      601 cgcgggccgc ggaggccgcg acgatcacaa tgacgtcggc gaacgtgttg aatgtcaccg
      661 agatcaggcc cagcgtcatg aactgcagcg cggcgtggcc ggcggccggt tccagaaatt
      721 gcggaatgaa cgccaggaag aaagccgcgg tcttgggatt gagcgcctcg accacgatgc
      781 cttcccggaa tgcccgcctg aagccgatgg cgcccgtcgg ctccgtcggc agatggccgg
      841 cttcgcggat ggttctgatg ccgagccaga tcagatagag ggcaccggcg agcttgagca
      901 tggtgaagag ctgtgcgctg gcaagaatga tcgccgagac gccgataccg ccggcgacca
      961 cgtgcacaag gccccccagt gcggtgccga aggtggatgc gataccggct cgtctgccgc
     1021 ccgagagcgt tttcgccgcc acatagaaaa tgccggggcc gggaacggcg gcaatcacca
     1081 ccgccgccag caggaacagc gaaatatcgt aggatgggtt gagcgcagcg aaacccatca
     1141 acggcttccg atgaagtgat gggtttcgct gcgctcaacc tatcctacga ctcttgcgtt
     1201 tctgttcaat gtgatctcgc caggcagaat gccaccacct gctcgagcgc ggtcttcatg
     1261 ggcgaggccg gaaacagcgc cagcgcatcg accgccatgg cgccgtaatg ctgggcgcgg
     1321 tggatggtgt cctcgagcgc gcggtgcttg gtcatcaggc cgatggcatg gtcgaggtcg
     1381 tgatcgccga tttcgccgcg ctccagcgcc ttgatccaga aggcgcgctc ggcatcgttg
     1441 ccgcggcgga acgccagcac caccggcagc gtgatcttgc cctcgcggaa atcgtcgccg
     1501 atgttcttgc cgagtttggc ggccttgccg ccgtaatcca gcacatcgtc caccagctga
     1561 aaggcgatgc ccaggttcat gccgaccgag cggcacgcgg tctgctcggc cttcgggcga
     1621 ttggcgatga ccgggccgac ctcgcaggcc gccgcgaaca actcggcggt cttgcccctg
     1681 atgacggcga ggtattcgtc ctcggtggtg gcggtgtttt tggcggcggc aagctgcatc
     1741 acttcgccct cggcgatcgt cgccgcggcg gacgacaaaa tgtcgagcgc gcgcagcgag
     1801 ccgacctcga ccatcatgcg aaaggcctga ccgagcagaa agtcaccgac cagtacgctc
     1861 gcttcgttgc cccacaacat ccgcgcggac aatttgccgc gccgcaattc gctttcgtcg
     1921 accacgtcgt catgcagcaa ggtggcggta tgcatgaatt ccacggaggc ggcgagcttg
     1981 atatgcccgt cgccggaata gccggtgaga tgggccatcg ccagcgtcag catcggccgc
     2041 agccgcttgc cgcccgacga aatcaggtgg ttggccacct ccgggatcat gatgacttcc
     2101 gaaccggttc gcgacagaat ggtggcgttg acgcgctcca tatccgccgc gacaagccca
     2161 accagctgat ctatcgaagc gcccgacggg ctctcgaagg gtacaataac cgccacgcgg
     2221 gtctccacat ttgcccaatt cggactatcc ttacgatata acaaatagaa agtgcgggct
     2281 aacgcggcaa gggcacgaag cggttgacac gcgtcgcgcg gccatcacct tgggtcgaca
     2341 aaggagtgtc aaagttgcgg gaattggttc ggactaacga tatggtgttg gtttcggcgg
     2401 tcggcgcgct actcgacggt gcgaatatcc atcacctggt gctggaccag aatatgagca
     2461 tcatcgaagg ctcgctgggg gtcttgccgc gccgtattct ggtccatgac gacgataacc
     2521 gcgaagcgcg ccagattctc cgcgatgcgg gcctcgctca cgaactgcgg gccgatgact
     2581 gatccggctt atgaattcac cgaggacgca tttctcggcg ggcgattgcg tctgcggcag
     2641 cccaaatcgg gtcaccgcgc cggccacgac gccatgctgc tggcggctgc gacgtcggca
     2701 cgcgcgggcg atcgggtcgt tgatttcggc gccggcgtcg gcgccgccgg tctggcgctc
     2761 gccaagcgcg tggcgggaat cgaacttgtg ctggtcgaga tcgatgaagc gctggcgcgt
     2821 ctcgcgcggg gcaacgcctt ctcgaatgct ataccggccg atgtcatctt gcttgacgtc
     2881 acgtcttccg ccgacaggtt tgccgcggcg ggtctgtctg ccgacagtgt cgatgttgta
     2941 ttgatgaatc cgccgttcaa tgatccggcg cggcatcgcg cttcgccgga cagggcccgc
     3001 gaggtcgccc atgtggcgac ggcagcgacg cttgcgagct gggttcacgc ggcgcgaaga
     3061 atcctgaaat ccggcggcgt cctctgcttg atctggcgcg cggacggcct tgccgaggtg
     3121 ctggcggcgc tcgatcgcgg atttggcagc ctcgccattc tgccggtcca tggcgacccg
     3181 aagaagccag cgatccgggt gctgatccgc gccatcaagg ggggaaaggc gccgacggaa
     3241 atacacgccg cgctgatgct caaggatgag tcagcgttgc ccaataaaca ggtagaggaa
     3301 ctattggccg gaaaggggat cttgcccttg gcgatgtccc ggaaggcatc gccggctatt
     3361 gcggaagcgt ctccgagcgc cgctctggca ctgaagtgaa gcgaatcgcc gtcaaaagtg
     3421 caccgatcag ggccatcagc agatagattt tcatgttctt ctccgctgca ccgttgcagc
     3481 ctgacaacga ccgcaaagca agcgccgttc cattccattg gatgccagtc gcgtgataaa
     3541 aattggttaa ttttgaggta atagcatgat ggagcaaacg gtcgatcgcg gcggactgcc
     3601 gggcctgatc gacaggctga tggagttcgt tcccgcgcgt ctaaggcgtg gggtagccgt
     3661 ggtccccgtg gtccgtctgt ccggcgtgat cggcgcggtg acaccgctgc ggcccggcat
     3721 gtcgctgacc ggcgtcgcca agacgcttga acgggcgttt gcgaccaaga atgccaaggc
     3781 tgtggcgctg atgatcaatt cgcccggcgg ttcgccggta caatcgcggc agatttacct
     3841 gcggattcgg cagctcgccg cggagaagaa acttccggtg ctggtgttcg tcgaggacgt
     3901 ggcggcatcc ggcggctata tgatcgcctg cgcgggcgac gagattttct gcgacccgtc
     3961 gtcgatcctg ggctcgatcg gggtggtcgg cggcgccttc ggctttcagg acctgatcaa
     4021 aaagatcggc gttgagcggc ggctatacac cgccggcgag cacaaggcga tgctcgatcc
     4081 gttcctgccg gaaaaccccg aagatgtcgc ccgcgtcaag gcgcttcagc gtgagatcca
     4141 tgccatcttc atcgccctgg taaaacagag ccggggcggc cgcctgaagg gcgccgacca
     4201 tgtcctgttt accggcgagt attgggcggg agaaacctcg gtttcgcttg ggcttgcgga
     4261 tgcgatcggc gatctgcgct cgaccctgcg cgcccgctat ggtgacaagg tgttgacgcc
     4321 ggtgatcgcg cctgcgacgg ggatgctgtc caacctgctg ggccgcagat cggccggggc
     4381 ggggacgctg acgtcgctgg atgggattgc cggtctgccg gacgagctga tttcggcgct
     4441 ggagacccgg gcaatttggg ccaaattcgg gttctaaggc gaaatactgc gccatttggc
     4501 cctagatatt gcgattgcgg gccgggccgg cttgcgcgac aatatgagtt ggggcgtggc
     4561 gtatgaacga caaggatcga ccgatgccgc cgctgatcgc attcgcaggc gccctgggtg
     4621 ggctggccgt ggtccgctgg gcttaccgga cagccctccg ggtcaacagg gaattggaag
     4681 aggcgcgcct ggctcgcctc gccgaagcgg acagcttggg cgagattccg acgctgcgac
     4741 gtgaccccat caccggcgcc tatcgcccgg gctaacggcg tcgcgaggtt cgacggcgct
     4801 cggactcctt gcccatcgcc ttgattcccc ggctcctcgc cgatacggtc ccgcgcgcct
     4861 gccggcccct cgcgaacccc gaatcggacg atggactcca tacccgccca catgcgcccg
     4921 gaacgctcgt tccagggctt cattctcgct ctgcagcggt tttgggctga tcagggctgc
     4981 gtgatcttgc agccctacga tatggaaatg ggcgcgggca cctttcatcc ggccaccacg
     5041 ctgcgcgcac ttgggccaag gccgtggaag gccgcctatg tgcagccctc gcgccggccg
     5101 aaggatggcc gctatggcga gaatcccaat cggttgcagc actattatca gtttcaggtg
     5161 atcatcaagc catcgccgcc ggaccttcag gaattgtacc tgaagtcgct cgacgccatc
     5221 ggcatcgatt caagcctgca cgatatccgc tttgtcgagg acgactggga aagcccgacg
     5281 ctcggcgcgt gggggctcgg gtgggaatgc tggtgcgacg gcatggaagt gtcgcagttc
     5341 acttatttcc agcaggtcgc gggcgtcgaa tgcgcgccgg tcgccggcga actcacctac
     5401 gggctcgagc gccttgccat gtacgtgcag ggcgtcgatc gcgtctacga tctcaatttc
     5461 aatggccgcg agggcgccga gaaggtcacc tatggcgacg tgttcctgca ggccgagcag
     5521 gaatattcgc ggcacaattt cgaatattcc gacgccgaca tgctgttcaa gcagttctcg
     5581 atggccgagg aggcctgcag gaaatatctc gaggccggat ggaagagcgg cagcaaccga
     5641 acggagcatc tgatggcgtt gccggcctat gaccagtgca tcaaggccag ccatgtcttc
     5701 aatctgctcg acgcccgcgg cgtgatctcg gtgaccgaac ggcagagcta catcatgcgg
     5761 gtgcgcgagc ttgccaaagc ctgtggcgag gcctggatgc ataccgaagc gggaggggcg
     5821 acatgagcga gcagccggcg ataggcggca gagcgccgat tccagtcgag gtcgaggcgg
     5881 gcaaggatta ttggtggtgc gcctgcggca agtccgctga tcagccgttc tgcgacggca
     5941 gccaccaggg ctccggcttt tcgccgctgc aatacacggc gccggagacc aaaaaggtgt
     6001 tcttctgtac ctgcaagcgc accgcaacca tgccgctgtg cgacggctcg catggccgac
     6061 tgccgacatc ggtttgacag cacgccctta gagctcttgc ttcgcttcca cctcgcgggg
     6121 aagggaacga gagagtggat ttgaaattaa aatgaccgat ctgcttctcg aacttttttc
     6181 cgaagaaatt cccgcgcgga tgcaggcgaa ggcggccgac gatctgcgcc gcatggtcac
     6241 cgacaaactg gtcgccgagg ggctggtcta tgagggcgcc aaggcgttcg cgacgccgcg
     6301 gcggctgaca ctgacggtgc acggcattcc cgcgcggcag tcggatctga aggaagaacg
     6361 caagggcccg cgcgtcggcg ggccggaggc cgcgatcgca ggctttctca aagctaccgg
     6421 actctcttcg ctcgatcagg caaaaatcca gcgtgatccc aaaaagggcg acttttatat
     6481 cgcgctgatc gaaaagcccg gccgcgccac gctggacgtg ctcgccgaga tgctgccggt
     6541 gatcatcaga accttcccgt ggccgaaatc gatgcgctgg ggcgaacgtt cggcacgatc
     6601 cggcgcgctg caatgggtgc ggccgctgca ttccatcgtc gccacgttcg gccttgaaac
     6661 cgaagcgcct gatgtcgtga agttcgacgt cgcgggcatc gaggccggcc agaccaccta
     6721 tggccaccgc ttcatggcgc cggcggcgat ttccgtgcgc cgcttcgagg attatgacgc
     6781 aaagctgctg gatgcgaagg tcgtgctcga tccgcagcgc cgcaaggaca ccattctcgg
     6841 cgaagcaaag acgttggcgc aggcgcaaaa ttacgagctg gtcgaggatc aggcgctgct
     6901 cgacgaagtc gcgggcctcg tcgaatggcc cgtggtgctg atgggatcgt tcgaccgcga
     6961 atttttgaca attccgggcg aggtgatccg cgccacgata cgaaataacc agaagtgttt
     7021 tgtggtcagc gaccccaaaa cccgcaagct gaccaacaaa ttcatcctca ccgccaacat
     7081 cgaggcctcc gacggcggca aggccatcat cgccggcaac gagcgcgtga tccgcgcgcg
     7141 gctgagcgac gcgaaatttt tctacgagac cgatttgagg accaagctgg aagaccggct
     7201 gccgaagttt gaaagcatcg tgtttcacga gaagctgggc acacagggcg agcgcatcgc
     7261 gcgcatcgag cggctggcag cggagatcgc gccgctggtc ggcgccgatg tcgaaaaggc
     7321 aaagcgggcc gcgcgtctgg cgaaggctga tttgctgacc gaagtggtcg gcgagtttcc
     7381 cgagctgcag ggcctgatgg gaaagtatta cgcgctggcg cagggtgagg atgcctccgt
     7441 cgccgccgcg agcgaagaac actacaagcc gcaagggccc gcggatcggg tgccgactga
     7501 tccggtgagt gttgcggtgg cgctggcgga caagctttat tcgctagtag gtttttggat
     7561 aattgacgaa aagccgaccg gatcaaaaga tcccttcgcg ctcaggcgtg ctgcgcttgg
     7621 tgttattagg gtgatttgcg acaataatat tcgtcttagc ctgtcggccg aattagacaa
     7681 ggcgacggcg gtatttcctc ccaatctgcc gaacgagcaa agtacgggag cctatgctgc
     7741 gcacctcatc tcgcagaaac cggactcggg gccaaaaaga gaaccgcgct acttcgtagt
     7801 tggtgagctt ctttccttct tcgccgatcg cctcaaagtc cagctccgcg agcagggcgc
     7861 ccgtcacgat cttgtcgacg ccgtattcgc gctcgaaggc caggacgacc tcttgatggt
     7921 tgtccgccgt gtcgaggcgc tcgggagatt tctcgatacc gacgacggca agaatttgct
     7981 cgccggcacc aagcgcgcgg cgaatatctt gcggatcgag gagaagaagg atggcaaggc
     8041 ttatgatggc gcgccggttg ccacgctcta cagcctcgcc gaagagaagg cccttgcgaa
     8101 agcgatcgat caggtgaaga cggaagccag cgccgccgtg gcaaaagaag attttgcggg
     8161 cgcgatgagc gtcatcgcaa aattgcggcc ggcggtcgat gcgttcttcg acaaagtgaa
     8221 ggtcaacgac gacgacccaa aagtgcgcga gaatcgacta aagctcttga atgaaatccg
     8281 cgccgccacg cgcgcggtgg cggatttttc caagatacag gattagcgct cgtaccccgg
     8341 atgctgcgca gcgccatcag cgcgttcacg cgcgtctacg acgcgccatg gcggtgcgct
     8401 gctgatccgg ggcccatgcg gcgggtccat cgctcggttg gtcccggctc tgcggcgcgg
     8461 cgtaaaggac gccgcaccgc gtccgggaca cgagggccca cgatggcagc ccattcaagg
     8521 tccgtgagtt catcccgcat gattcgaggc tccggtttgg gcgcttgaat cacgaatgac
     8581 ccaagtcaga cattccggaa atttctgaga ggctactgcc cagacaattg agcgccatcc
     8641 cggtcgttag ccatagcgct cctgccttgg gccgcttgtc cggttcggat ttggggttgc
     8701 gggctcgcga actcggatat caaattgtcc cattcctttg cttctctaat ccagaatgca
     8761 tcattctcgt cttcaaacgc cgcgcgttgg cggcagaaag cggccatggc acggcaatgt
     8821 gcaagcatat ccattcgaca ttccccagtc ggagtaatgc taaacgtcat gcacacaata
     8881 cagcggtttt gttacagacg cttctctgcc gctctccgta agactacgga tagaactata
     8941 gtcctgatcc acaccccagt gtattatcgc ccccatggaa aagtccgatg ttatctgtcc
     9001 cgaatgcagt gctggctatc gccggaccga gctaacctca aaaccccggg gccaatggcg
     9061 aatttcgctg cttggtctgc aattatctgc ttgaggtttt cgacggcaca aaggacgtgg
     9121 cccttcgcct cacggtccag cccgaaaagt ggttcgaata gcggggacaa accgatgtag
     9181 acggctctgt ggagtatcga gaggtacggc atgttcaggc cggcagcgtt gtccgggctg
     9241 gtctcgcacg acctggccgt gatcaagggt cacggccgct atcagttcct gcgcgatatg
     9301 ccgccttctc ctccgcccca cgccgactga cgtagacttg agcctgctct caggcaagat
     9361 gcgcttcggg aagttcctgc gtggttgctt ggccggcaaa gattccgagc tcagagcgca
     9421 acggaagcga cagcacatgt gtcagcttcc ggcaatgcgc cccgaagacc gctgctctga
     9481 cgagatccgg tgcggaacgc gagcgaaatc gtcatattta cccgacgcat actgcatttt
     9541 tggcgtgccg cgatgtcgga gcttcgcgaa gccggctcaa attgctataa gggtaaaaac
     9601 ggaaacagaa acggtgcaaa actggtaaga aacaaaccgt cgaagaccgt gtcacgtctc
     9661 ttgagcgggc agcagtgtgc gatgaatgat gaaattaccg acgttgctgg cgacagcaaa
     9721 gccccggatt tggagcggac aggagcctca cccgaggcga tcatctcgca ctatgacaaa
     9781 ggcgaagatt tctttaaact cgtgcttgga cccgagttga tctattcatg cgcgctgttc
     9841 gagagtgacg atgatctcgc ggaagcgcag cgccgaaaac ttgattatca tatcgaagcg
     9901 gcgaacgccg ccaacgcaaa acgcgtgctc gacgttgggt gcggatgggg agccatgctt
     9961 cgccgccttg tcgatcacgc ggaggtgcca cacgttgtcg gtttaacgct cagtccatcg
    10021 caggctaact ggatccgccg ccaaccgcga cccggactcg aagtggttga gcaagactgg
    10081 cgcgatcatc agccaaccca gcggtacgat gcgattatct cggtcggtgc gttcgagcac
    10141 ttcgtccaaa agggcctcga tcctgtgaag aagctggatg cctatcgtga gttttttgac
    10201 ttttgcgatc ggatgctggt agcggaggga aaaatatcac tgcagacaat cgcgttttcc
    10261 gaaccgcacg agggtcatcc gctgctggaa aagacgtttc ctgatagcga tctgccattg
    10321 atctgggagc cgatcgccgc ggccgaaggg aaatttgtgc tgaccacgct gcgtaacgac
    10381 cgcgatcact attaccggac gctgcgagct tgggagcaaa atctgatggc tcattacgct
    10441 gaggcgatcg agttagtcgg agaatcgacc gttgccgact tccgccgcta tctacgcatt
    10501 tccgccacgg gttttagaat gggaatagtc agcctgttac gaatgagttt tcagaaaagg
    10561 atgtgaattc taggccgcca gtctccgcat ccggacgatg acgtcaatct tccgggcgcg
    10621 aatgtcgtcc agtagtcgtg aaacatcacc gatgtcggtc gtttgcggta tcggccgctc
    10681 caatcgctaa agcgggcaag gccgactccg cggcgaacca atggaaccga tgtgtccgtt
    10741 tcccggtgcg cgccgaactc gattggccga agcgcgttga tcttggggat cactaccagt
    10801 tcgaggccgc cgtttcccgg tgccactgat cgcgagagtt actgccatgt cggacaagat
    10861 ttctgccgaa gacaatcatg cccgtcgtcg cgtaggcgtc catgacagcg agatgagtta
    10921 cgtcaccgtt ggtggcgggg atccgatcgt tttcctgcac ggaaacccga cctcatccta
    10981 tctttggcgc aacgtcatcc cccacgtcag cgatcttggc tggtgcctcg cgccagacct
    11041 catcggtatg ggccagtcag ccgtctctcc aagcggtgct taccgctttt tggatcatgc
    11101 acgttatctc gacgcatggt ttgaggcgct ggacttgcgt cggaacgtta ccctggtgct
    11161 gcacgattgg ggatccgccc tcggctttca ttgggcttca aggtatcccg agcgagtacg
    11221 cgccatcgct tacatggagt cgatggtcaa gccgcgcgac tgggaagacc ttccctccag
    11281 tcgggcgccg atctttcgcg acctgcgctc cgagaagggt gagcgcatga tcctggacga
    11341 gaatgccttc atcgagattc ttttgccgaa gctcatcatt cgtaccctga ccaaggctga
    11401 gatggatgcg tatcgtctcc catttagaga gccagagtcg cgttggccga cgcttgtctg
    11461 gccgcgcgag ctgcctatcg ggggcgaacc tgccgacgta gtgaaaatct tcgaaggcta
    11521 cgggcaatgg cttctccgga gtccgctgcc gaagttgttc atcaacgccg agcccggatc
    11581 gctgttgatc ggacgagcac gcgacttctg ccgttcctgg ccaaaccaac aggaatcaac
    11641 cgttcggggc atccatttca tacaggaaga tagtcctcat gagatcgggt cggcgcttgc
    11701 gcgcttcatc aggcaagcaa ggaccgcgat ctaaggtaat ccaacatgtc cgcctatctg
    11761 aaggttttcg gacctggcgc gatgtccgac ttgagtctgt tttgcgctcc aaagcggaca
    11821 tcggccgacc aatctgaatt aatgggttca cgccctggtt gggtcgcggc tctgcggcgc
    11881 accgccaaga ggcgctgcgc cgcgtccggg acacgaggaa cgtgcgctcc ccgctgagtg
    11941 gcgatttgcc cgtcgggcag cgcttaggat gagtggagcg aaagcgatac ccgcggagcg
    12001 ttgctgcctg ctaggcagcc gacacagtca ccgctgccgt gacaggagcg gcggtcagcg
    12061 agcgcccgat aaccgccact actggcacgg tgggttggga tagtatccgc agcgataagc
    12121 tccgtaagct ccggcggcac cagcaccgac tgcggcagct ccgacgccta ccgccactcc
    12181 acggcgtacg gctgcaccac ggtagacgcc accgcgatag acgccaccgt gataaacgcc
    12241 agcgcggcca actccaacgc cccttcgata tgcgaaggcg tcggtagaaa tagtcgcaat
    12301 gcaggcgatg ccgacgataa ccgatgcagc aagcgaaacg atggttcgac gtgacatgat
    12361 taatcctttc tcgggccgat cagttcgtac tcgaagttta gatcccttcc tgacaacttg
    12421 tgctgtgtct atgcaatttc ggccggttcg acgcgggctg cgggtttttt gtttgcgatt
    12481 aatagaactc ctgcattcgc ataggcgttg ctttatcgcg cccttgaagt gcaatagaac
    12541 tcttcgcgag taagcacttt acgttctgag gccatgatcc cagtatggcc ccgctcccga
    12601 attatagatg ttcaattttg ctcagaaccg cgcaaaactc ccgagtccgg gcgttgcggc
    12661 gcatgagtcc gttggcgctt tttcggacct ggcgcgatgt ccgacttgag tctgtcttgc
    12721 gctccaaagc ggacgtcggc cgaccaatct gaattaatgg gttcacgccc tggttgggtc
    12781 cggcgctgcg gcgcaccgcc aagaggcgct gcgccgcgtc cgggacacga ggaacgtggg
    12841 ctcgcagctg agtggcgatt tgcccgtcgg gcagcgctta ggatgagtgg agcgaaagcg
    12901 atacccgtca atatcgttgc gcgtacgcgg cgatttgttg tgctttctca agctgatttg
    12961 ggtcgtccag tcctcgttgc aaaaatattt cggttttcac gccccccaaa tcatctctag
    13021 aacttttcgc atcccacccc acaagagggg cgtatcgcga tcgtcacgga cgtaggggag
    13081 ggatgcgatg gacgcggcag cgttttgcgc gcgatgggat cgcagggcgg tttctgagga
    13141 atccgtgagc gatcaacagc gcgctgacga gagacgattg ctgcggacgg caaaatcgtg
    13201 tggtcctgac gccccgacgc tggcgtcaag ttcgcggagt tgcgtcggcc cgaccgggtt
    13261 tagacaaaac cttgctctgc ggatgacggt ggcaaaagag cccggtcacc ggggagagca
    13321 cgacataagc cgtaaaacca ttgcgtgcgg gaatgccggg cgattccggt gtactcgttg
    13381 ttactcgtgt gcgttctact actacaagtg cacacgaggc cgcgggtgca gcgggcaccc
    13441 ggcgttcccc acgccctcaa agagggcgga gatttcatgc acgactcggg cgcagcgcgc
    13501 cgcgagatcg tgaaactgca tttggacgtc attgcgagcg cagcgaagca atccatggct
    13561 gcaacaagaa gaaagaagaa agatggattg cttcgctgcg ctcgcaatga cggttgtctt
    13621 tggctgtttg aaaattgaac agggaattag agtgcgcagc actcttaacg tgcgccacaa
    13681 aacgcacccg tcatgcccgg acttgatccg ggcatccatc aatcttcgta aaacggtttt
    13741 cgaagaagat tgatcactgg gtcaagcccg gtgatgacga tctcaactcg tttgacaggc
    13801 ttcgcgaggc gcgacttgtc cgctgtagct cagcgagcga aggcggaacc gataacgcaa
    13861 tccatgacga agtcagaagg tgatgtggct gaaacgtcgg taaaaagaaa agccccgccc
    13921 ggcgggggaa ccgggcggga ctttcatgtc cagtcgctct ggcggcgcgg cgcccggaca
    13981 tggtctcatg ccgttacgtg cgggccgtta ctcgaccacc tgcacgatgc ggcgcgagcg
    14041 cggttcgacc agcacggtgc ggccgttcac caccgtgtag cgatagggcg tggggccgaa
    14101 tgcctgcggc acgtcgtaat aggtgacgcc ggcttccggc agcacgccgc caacgatcac
    14161 gcgatccggg atggtgtaag tcggcacccg ctcgcgcacg atgtattcgc ggaacgccgg
    14221 ccgctgatcg acggcgatgc cgac
//
LOCUS       DATNOI010000336         7183 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1969095,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000336 DATNOI010000000
VERSION     DATNOI010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969095"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VN968_13365"
     CDS             <1..819
                     /locus_tag="VN968_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXL30264.1"
                     /translation="HRFFEPAMDASAKARLTMEQDLRQALADGGFEIHYQPLVNLRHN
                     EVTGCEALLRWRHPERGMISPAEFIPVAEDTGLINELGEWVLRTACAEAASWPDHIRI
                     AVNVSPVQLKCQTLALKIIGALAASGLPASRLEIEITEAVLIRDDEAALAILHQLRVI
                     GVRIALDDFGTGYSSLSYLKRFPFDKIKIDRCFISDIAEIDGSSAIVQAVVNIAAARN
                     MTTTAEGVETLEQQELLRTLGCTEMQGYLFSAAKPGPEVRKLFGPRPERAAAVA"
     gene            complement(867..1946)
                     /locus_tag="VN968_13370"
     CDS             complement(867..1946)
                     /locus_tag="VN968_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXL30265.1"
                     /translation="MRPLEFGWYLPTHGDTTAYGVMEAQVPGSPELCGRVVHAAEAAG
                     FEYLLIPVGSVCWEAWITGAFMAARSSKIKPLIAARPGYINPVLLAKMISTFDQMSGG
                     RICINLIAGQNESEVEGEGVRYAKQERYALMEEEVSILKALWTARGPVNFQGKFHTLS
                     GAHIRPRPLQQPFPKFYLGGGSREAWQMSAKHSDVHLFWGDLPERIAANIAEIRAMAR
                     PHDRENTIGFGMRLQVICRENEKDAWEAADILVRNATERQKQEMKTLYNNSVANQRVQ
                     QLAREHGDLLLPHLWTGITRVRPGAGIAVVGNPSQCADTLQQFIDAGCHSFCLSGYLH
                     DEEAERFGRLIRPILAENNRGRLAA"
     gene            complement(2151..4040)
                     /locus_tag="VN968_13375"
     CDS             complement(2151..4040)
                     /locus_tag="VN968_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXL30266.1"
                     /translation="MKGASATFAWGNLSEDFALFRTLSPEQRRKAFSAMVRRDLVRGQ
                     TLVAQGGASDSLFMVLHGALAVRRTGDIEPIAELRAGELVGEIGFFANVPRTADVIAI
                     RDTSVLVLTRAAYQNLAEDAPAIVEALLAALARRFAKETARITPVRASPKARTVALID
                     GGREPLPGAFDRRMRDGLAATDAEIVDPARVNAMFPGRTLDTPEVADWLNKLEHAAPL
                     VVYLGGREASAWARKAIRQADMVVFACRGDAPAEALTEVEAFACDVHPVSARRLVRIH
                     DRRSGEVSGTTAWLARLPCFMHHHVALEDQIDIDSLIRFLFGRAIGFVAAGGGSFGTA
                     HVGIYKAFRERGVMFDVFVGTSVGSAMAAGFAKNLEAEDLERGTHEIFVRSRSFRRPT
                     WPRYALLDHKAFDRALAHQYGHDCRIEDCWRPFAAVATNLSTHNLELIRSGRLWQAVR
                     ASSAIPGLLPPFYTPEGAMLVDGCLIDNVPLASMHQLKSGPNLVVHFGEPAAEMFDVE
                     YAALPGRLELITTMLMPFRKKLLPAAPSAVNVLWRSLVAHQRYDTLPTAPLDMVMRPP
                     SPFGIDVTEFHRHTEIFEASYFWARQAIVALEAEGNSAIAAILATGRAAMERPRDLVG
                     LRTAL"
     gene            4324..4400
                     /locus_tag="VN968_13380"
     tRNA            4324..4400
                     /locus_tag="VN968_13380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4358..4360,aa:Arg,seq:ccg)
     gene            complement(4605..5294)
                     /locus_tag="VN968_13385"
     CDS             complement(4605..5294)
                     /locus_tag="VN968_13385"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HXL30267.1"
                     /translation="MDHDAHPARRPKLISFDVFGTLISVRDSSYSAFERILADAGARH
                     VDVKAFWEHWEHRNIAHYWDAYRSYREICELSLAETFAHFHVVGDSRSIDRYFEAFPR
                     FFLYDDVVRTLDHLSRGCRLAVVSNIDDDLLALTPLQRNFDLVCTAERAKGYKPDGTL
                     SRYLIENAACARDEILHAGQSQFTDLVGGKPLGLTIAWINRRQLALDASVPRPDFIFP
                     DIQSLSRVVGF"
     gene            complement(5395..5694)
                     /locus_tag="VN968_13390"
     CDS             complement(5395..5694)
                     /locus_tag="VN968_13390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018099090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HXL30268.1"
                     /translation="MLTITAVIRAKKGHEDTMRRALLEVAENVRSNEPNTIGFFVSQD
                     QTNPGVFTTYERFVDQAAMDAHNNSQVVARFFGIAKPILDGDVVLVTGTEISAKA"
     gene            6035..6229
                     /locus_tag="VN968_13395"
     CDS             6035..6229
                     /locus_tag="VN968_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30269.1"
                     /translation="MNGNLSLAELDDRIAILRDNLRQLVEQAAAASGSQNEERIADRI
                     AQQTEELDKLILERDSRAKK"
     gene            6546..>7183
                     /locus_tag="VN968_13400"
     CDS             6546..>7183
                     /locus_tag="VN968_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL30270.1"
                     /translation="MTVQDLPSKVSTPEFEERSVPACIAVICAFAATGMLATNIFLPS
                     LPSMAATLQVSSAAVTSAITVYLAIFAVGQLVVGPLSDRFGRRPVILIGLSLFVAGAI
                     GCAFARDLSDLLIGRSIQASGGCAAMVLSRAIARDLFDRQLTRVMAAITIATAAAPGF
                     SPLLGGALDHFLGWRSEFIFVAIFAVCTVTAYVSFVGETIRSARSSMNPLTIA"
BASE COUNT         1377 a         2215 c         2238 g         1353 t
ORIGIN      
        1 catcgcttct tcgagccggc catggatgca agcgcgaagg cgcggcttac catggagcag
       61 gatctgcgcc aggcgctcgc ggacggcggc ttcgaaatcc attaccagcc gctggtcaat
      121 cttcgtcaca acgaggtgac cggctgcgaa gccttgctgc gctggcgcca tcccgaacgc
      181 ggcatgatct ctcctgccga attcatcccg gtggcggaag acaccggcct gatcaacgaa
      241 ctcggcgaat gggtgctgag aaccgcctgc gccgaggcgg cgagctggcc ggatcatatc
      301 cggatcgccg tcaacgtgtc gccggttcag ctgaaatgcc agaccctggc gctgaaaatc
      361 attggcgcgc ttgccgcctc cggccttccg gcgagccgcc tcgaaataga gatcaccgaa
      421 gcggtgctga tccgcgacga cgaagcagcg ctcgccatcc tgcatcaact ccgcgtcatc
      481 ggcgtccgca tcgcactgga cgatttcggt accggctatt cctcgctgag ctatctgaag
      541 cgcttcccgt tcgacaagat caagatcgac cgctgcttca tcagcgacat cgcggaaatc
      601 gacggctcat cggccatcgt gcaggcggtg gtgaacatcg ccgccgcgcg caacatgacg
      661 acaaccgcgg aaggcgtcga aacgctcgag caacaggaat tgcttcgcac gctggggtgt
      721 accgagatgc aaggctacct gttcagcgcc gcgaagcctg gtccggaagt gaggaagctg
      781 ttcggcccgc ggcctgagag agcggcagcc gtggcttgat ggggctgttg ccggcggcca
      841 gtcgccgcat ccgatccgcc atggcgttac gccgccagcc ggccgcgatt gttctcggcg
      901 agtatcgggc ggatcagccg accgaaacgc tcggcctcct cgtcgtgcag atagccggac
      961 agacagaacg aatggcagcc ggcgtcgatg aattgctgca gggtgtcagc gcattgcgag
     1021 ggattgccga ccacggcgat cccggcgccc ggccgcacgc gggtgatgcc ggtccataga
     1081 tgcggcagca ggagatcgcc gtgttcgcgg gcgagctgct gcacccgctg gttggccacg
     1141 gaattgttgt agagcgtctt catctcctgc ttctgccgct cggtcgcgtt gcgcaccaat
     1201 atatcggcgg cctcccacgc gtccttttca ttttcccggc agatcacctg caggcgcatg
     1261 ccaaagccga tggtgttttc gcgatcatgc ggccgtgcca tcgccctgat ctcggcgatg
     1321 ttggccgcga tacgttccgg cagatcgccc cagaacagat gcacgtcgga atgcttcgcc
     1381 gacatctgcc aggcttcgcg cgagccgccg ccgagataga atttcggaaa cggctgctgc
     1441 agcggccgcg gccggatatg cgcgcccgac agcgtgtgaa acttgccctg gaaattcacc
     1501 gggccgcggg cggtccacag cgccttgagg atcgagacct cctcttccat cagcgcgtag
     1561 cgctcttgct tggcgtagcg cacgccctcg ccttcgacct cgctttcgtt ctggccggcg
     1621 atcaggttga tgcagatgcg cccgcccgac atctggtcga aggtcgagat catcttggcc
     1681 agcagcaccg gattgatgta gccgggcctc gctgcgatca gcggcttgat cttcgaggag
     1741 cgggccgcca tgaacgcgcc ggtgatccat gcttcccagc aaaccgagcc caccgggatc
     1801 aggaggtatt cgaagcccgc cgcctccgcc gcgtgcacca cgcggccgca caattcaggc
     1861 gatcccggca cctgcgcctc catcacgcca taggccgtgg tgtcgccatg cgtgggcagg
     1921 taccagccaa attcgagcgg acgcatcgac gatctcctgt tagcgcggat ttggattgcg
     1981 cagcttgctc gggacgtagc ttacaggctg caccccgccg ggcaatcggg caggcgaaat
     2041 gcatgcgttg ccgtttcttc tatcgcatga gcgcagggag cgctctcggt gctgaccggc
     2101 tagccgggaa attgcgcgcc atggtcccgg cccgggcgca gcttggctgg tcagagcgcc
     2161 gttcgaagcc cgacgaggtc gcgtggtcgt tccattgccg ccctgccggt ggcgaggatg
     2221 gcggcaatgg ccgaattacc ctccgcctca agcgcaacga tggcctgccg ggcccaaaag
     2281 taagacgcct cgaaaatctc ggtgtggcgg tggaattctg tcacatcgat cccgaacgga
     2341 gacggtggcc tcatgaccat atcaagcggc gcggtcggca gagtatcgta acgctgatgc
     2401 gccacgaggc ttcgccacag cacgttgacc gcgctcggcg ccgccggaag cagcttcttg
     2461 cggaacggca tcagcatcgt cgtgatcagc tccagccgtc ccggcagcgc ggcatactcg
     2521 acgtcgaaca tctcggccgc tggctcgccg aaatgcacga ccagattggg accgctcttg
     2581 agttgatgca ttgacgcgag cggaacgttg tcgatcaggc atccgtcgac aagcatcgcg
     2641 ccctctggcg tgtagaacgg cggcaatagc ccggggatcg cgctcgaggc acgcacggcc
     2701 tgccagagcc gtcccgaccg gatcaattca agattatggg tcgaaagatt ggttgcgacc
     2761 gccgcgaaag gccgccagca atcctcgatc cggcagtcgt ggccgtattg atgggccagc
     2821 gctcgatcga acgccttgtg atccagcagg gcgtagcgcg gccaggttgg ccgccggaag
     2881 ctgcgactcc tgacaaagat ctcgtgtgtg ccgcgctcga gatcctcggc ctcgagattt
     2941 tttgcaaatc cggccgccat ggccgagccg acgctggtgc cgacaaagac atcgaacatc
     3001 acaccgcgct cacgaaaggc cttgtagata cctacatgcg ctgttccgaa gctgccaccg
     3061 ccagcggcaa cgaatccgat cgcgcgaccg aatagaaacc ggatcagact gtcgatatca
     3121 atttgatcct cgagcgcgac atggtgatgc atgaagcagg gtagccgggc aagccacgcc
     3181 gtcgtgccgc tgacttcacc gctccgtcga tcatgaatgc gaacgaggcg ccgcgctgac
     3241 accggatgaa catcgcacgc gaaagcctcg acctccgtca aggcttccgc gggcgcatct
     3301 ccccggcacg cgaacacgac catgtcggcc tggcggatag ccttgcgcgc ccatgccgaa
     3361 gcttcgcgac cgccgaggta aaccaccagt ggcgcggcgt gttcgagctt gttgagccag
     3421 tcggcgacct cgggtgtatc cagcgtgcgc ccgggaaaca tggcattgac gcgggcggga
     3481 tcgacaatct cggcatcggt cgccgcgagc ccgtcacgca ttcggcgatc gaaagcgccc
     3541 ggcaacggct cgcggccgcc gtcgatcaat gccaccgttc gcgccttcgg cgacgcgcga
     3601 acaggcgtga tgcgcgcggt ctccttggcg aaccgccgcg cgagcgccgc cagcagggct
     3661 tcgacaatgg ccggagcgtc ctccgcaagg ttctggtacg ccgcgcgcgt cagcaccagc
     3721 acgctggtgt cgcggatggc aatcacatcg gcggtgcgcg ggacgtttgc gaagaaaccg
     3781 atttcaccca ccaattcgcc ggcgcggagt tcggcgatag gttcgatatc gccggttctg
     3841 cggacagcga gcgcaccatg caacaccatg aacagtgagt ccgaagcacc cccttgtgca
     3901 acgagcgttt gcccgcgcac gagatcccgg cggaccattg cgctaaaagc cttccgccgc
     3961 tgttccggac tgagcgttct gaacagcgcg aagtcttccg acaggtttcc ccacgcaaat
     4021 gtcgcgctgg ctcctttcat tagcgaaatc cagttcgatg gcacggtgca atgttagcgt
     4081 tgggctgcct tcactgcgag cagtatcagg atcgatgcgc cagcccgccg cagcaatggt
     4141 ttttatggaa gcgctgcaac atgaattgcg aaatccgctg gctggcgccc aagcgctacc
     4201 ttctggcgca ccccgagacg gctcaaacgt ctggccgttg cctttccagc cctgatcctc
     4261 gcccaaatca accctggttt ggccgttgcc gacctcagac caatcggcta aatgaaccgc
     4321 aacgcacccg tagctcagct ggatagagcg ttgccctccg aaggcaaagg tcacacgttc
     4381 gaatcgtgtc gggtgcgcca tttttccaag aagtgcgcgc gttgcggccg agttcatgtc
     4441 gccggcaccg gcgacgaggt cggctggtgc gcgtgtcttc atccgagcgc ccgcccctga
     4501 ataagcacca gtgctagtga tggtgccaag gactgccagt agctactatt tgacgcgtgg
     4561 cttggctaac gccgaggttg cgtgggacat gcgcgatgtg cacttcaaaa gccgacaaca
     4621 cggcttagag actgaatatc cgggaaaatg aaatccgggc gcggaaccga agcgtcgagc
     4681 gcaagctggc gccggttgat ccaggcgatg gtcaatccga gcggcttgcc gccgacgaga
     4741 tcggtgaact gcgattgtcc ggcgtgcagg atttcgtccc tggcgcaagc tgcattttcg
     4801 atcaggtagc gagacagcgt gccgtcgggc ttgtagcctt tcgcccgctc tgccgtgcac
     4861 accaggtcga agtttcgctg caacggtgtc agcgccaaca gatcatcgtc gatgttggac
     4921 accaccgcca gcctgcagcc gcgtgacagg tgatccagcg tgcggacgac atcgtcgtag
     4981 agaaagaagc gcggaaaggc ctcgaagtag cgatcaatcg agcggctgtc gcccacaaca
     5041 tgaaaatgag cgaacgtttc ggcaagcgag agttcacaaa tctcccgata cgaccggtag
     5101 gcatcccaat agtgcgcgat gtttcgatgc tcccaatgtt cccagaacgc cttgacgtca
     5161 acatgacgcg cgccggcgtc ggccaggata cgttcgaagg cactgtagga gctgtcgcgc
     5221 acgctgatca acgtaccgaa aacgtcgaat gatatcagct tcggccgccg tgcagggtgc
     5281 gcatcgtgat ccatcaatcc gattcctgct gacagtttgt ttcaaaaata ggggcaagtt
     5341 gccgtaagtg aacctttcgg cgcggtcatg ggtatttccc gctgccgctg gcggtcaggc
     5401 cttggcggaa atctcggtgc cagtgaccag aacgacatcg ccgtcgagaa tgggctttgc
     5461 aatgccgaag aagcgggcca cgacctgcga attgttgtgg gcgtccatgg cggcctgatc
     5521 gacgaacctc tcataagtgg tgaagacgcc ggggttggtc tggtcctgcg acacgaagaa
     5581 gccgatcgta ttgggctcgt tgctgcggac gttttcggcg acctccagca gggcgcggcg
     5641 catggtgtct tcgtgcccct ttttggcgcg gatgacggcg gtgatggtca gcatgacaga
     5701 gccctttcga tgatgaactt tcgcaagacg gattttgacg ccgtaaccga tcgacgatgc
     5761 ggcatcattg agcagaccga cgggagccca tgttagcgcg cgtcgggcga tgcgcaactc
     5821 ggactgagtc gcagcgcggt cccgacgggt gcagggcgtt tagagggcga gtagccgcct
     5881 ggccggtgca gcttccactt gcaggtagta tcggtatggg ccgacaatgg agactgaacg
     5941 aagcccggca aaatcgagtc tgcggcccgt tctcgaagcg aactggagcc agagtcagat
     6001 tcgacaagcc acgtggtggg caaggagagc gacgatgaat ggtaacctat cccttgcgga
     6061 gctcgacgat cgcatcgcga tcctgcgaga taaccttcgg cagcttgtcg agcaagcggc
     6121 ggcggcatcc ggcagccaaa acgaagagag gatcgcggac cgtatcgcgc aacagaccga
     6181 agaacttgat aagctcattc tggagcgcga tagccgtgca aagaaataat ccgatctgac
     6241 gggccatggt tgatcatcga tcgcggtgcg tggcgcacgg cgagcaagcg tgaggctgct
     6301 gcgcggcgtt tctctcgcgc gaacggtgtg agatccgctg gaccgcggcg cttccgatag
     6361 acttatggcg tgggcgccgc tcgaatattc catcacctcg gtctaattcc ctccgtttgg
     6421 aggacgacat ccaaaattca attgttcagg atggtcgaca cgccgggacg gtcgagccgt
     6481 agcaccaccg catgaactca ggggcagagc ccggtctttg acgacgaatt tgagggcgca
     6541 tcaccatgac cgttcaagat cttccgagca aggtgtcgac gcccgagttc gaagaacgtt
     6601 ccgtgccagc ctgcatcgcg gtgatctgcg cgtttgctgc gacgggtatg ttggcgacca
     6661 atatttttct accctcgctt ccgtcgatgg cggcgacgct ccaggtatcg agcgcggcgg
     6721 taacgtccgc catcacggtt tatctggcga tttttgccgt cggacaattg gtcgtcggtc
     6781 cgttgtcgga tcggttcggc cgacggccgg taatcctgat cggattgagc ctttttgtcg
     6841 ccggcgcgat cggttgcgcg tttgcccgcg atctttcgga tctgctgatc ggccgatcca
     6901 tccaggcctc cggcggatgc gcggctatgg tgctgtcacg cgccatcgcg cgcgacctgt
     6961 tcgatagaca gctaacgcga gtcatggcgg cgataacgat cgcgacggca gcagcaccgg
     7021 gtttctcacc gctgctgggc ggcgcactcg atcatttcct gggctggcgg tcggaattta
     7081 tctttgtcgc gatttttgcg gtctgcacgg tgacggccta tgtatcgttc gtgggcgaaa
     7141 ccattcgatc cgcccgcagt tccatgaacc cactgacgat cgc
//
LOCUS       DATNOI010000337         5893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1789202,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000337 DATNOI010000000
VERSION     DATNOI010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789202"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="VN968_13405"
     CDS             complement(<1..1105)
                     /locus_tag="VN968_13405"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXL30271.1"
                     /translation="MQSKAQIDQVLRQKSDATEIPGLVAVAATSTEVTYQGAFGKRDL
                     SKDDAMTTDSVFWIASMTKAITAAGAMQLVEQGRLALEAPIGQLLPDLAAPQVLEGFD
                     ANGEPKLRPARNPITLRHLMTHTAGFCYDLWNGDMVQYMAKTGTPGIISCQNAALKTP
                     LTSDPGTRWEYGINIDFVGKAVEAASGKRLDAYLRDHLFAPLGMTDTAFKIGDAQRKR
                     LVGMHARGPDGSLAPIPFELEQDPEFHMGGGGLYSTAGDYIKFTQMILNKGRGNGNQL
                     LRPETVAMMGQNHIGELNMTKMISALPDFTNHVDLYPDMDKKWGLSFLINTAKTPEGR
                     SPGSLAWAGLANTYFWIDPARDVTGVILMQVLPF"
     gene            1457..1777
                     /locus_tag="VN968_13410"
     CDS             1457..1777
                     /locus_tag="VN968_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30272.1"
                     /translation="MISTWSEWKRYPRAGRGENIEAPITPGIYEVRYAGTGALHSFDA
                     VDNVAQALAQLPVGSKSWFGRRGAQAAPELEYRTCATSSKADAKAAAERMIGRRDTYM
                     SGAA"
     gene            2001..3497
                     /locus_tag="VN968_13415"
     CDS             2001..3497
                     /locus_tag="VN968_13415"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HXL30273.1"
                     /translation="MSPTAAARPSQAPAPSLRDRLKDASLLREQCYIDGAWTGTPANP
                     VTNPVNGVELAKVPKMNTAEATQAVEAAERAFPAWAKLTAKQRSNILRKWFDLIIANR
                     EDLALILTSEQGKPLAEALGEVDIGGAYVEFFAEEARRVYGETIPTQRPDARLLAIRQ
                     PIGVCGAITPWNFPCSMITRKVSPALAAGCTVVLKPANETPLTALALVALAEKAGVPK
                     GVFNILTGNSSAIGKVLCEHPAVRFVGFTGSTEVGKILYQQAAVGVKKLGLELGGNAP
                     FVVFDDADIDAAVEGAMVSKYRNMGQTCVCANRIYAQDKIYDQFVEKLSRRVAAMKIG
                     DGTETGVTQGPLINMAAVDKVEKHIADAVKHGAKIVTGGKRHSLGGSFFEPTVLSNVA
                     PDALVSQEETFGPLAPVFRFKDEADVIAMCNNSPFGLASYFYSRDLGRVWRVAEALES
                     GMVGVNTGLITTEVAPFGGVKESGLGREGSRHGMDEYVEIKYVMMAGI"
     gene            complement(3576..3758)
                     /locus_tag="VN968_13420"
                     /pseudo
     CDS             complement(3576..3758)
                     /locus_tag="VN968_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139795.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3852..4646)
                     /locus_tag="VN968_13425"
     CDS             complement(3852..4646)
                     /locus_tag="VN968_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXL30274.1"
                     /translation="MTDTDPVLVERDGPVTIVSINRPHCRNAVDGATARKLFDAFRAF
                     DADAEASVAVFTGVGGTFCAGADLKAVASGDPNKKREIGGHDSIAPMGPSRLRLSKPV
                     IAAIEGFAVAGGMELALWADMRVAAEDATFGVFCRRFGVPLIDLGTIRLPRLIGHSQA
                     IDLILTGRPVGGAEALRMGLANRLVPNGETRARAIALAKDIARFPQTCMLSALRQWDL
                     EEEDAIANEMRGGLEVIASGETLSGAARFASGVGRHGAFGGNTVDQ"
     gene            complement(4682..5128)
                     /locus_tag="VN968_13430"
     CDS             complement(4682..5128)
                     /locus_tag="VN968_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL30275.1"
                     /translation="MTGASGRRMTGRQTIGQVALLVRDYDEAIDYYIGILGFTLVEDI
                     YIAAQDKRWVTVAPPGSTETRLLLARAAGKEQSARIGNQTGGRIFLFLHTDDFWRDFH
                     SYKAKGVVFVREPMAESYGTVAVFKDLYGNLWDLLQPNADADSDSK"
     gene            complement(5180..5692)
                     /locus_tag="VN968_13435"
     CDS             complement(5180..5692)
                     /locus_tag="VN968_13435"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HXL30276.1"
                     /translation="MTLTVREMTIPEVDIIIEYFLQSTPEHLELLGVDPTCLPDPEIW
                     RERFRQDYARPIQQRGRIAVLWLSGDQPVGFSTSDKITYGEQAHMHLHVIDPERRSRG
                     IGAEGVRRSVDIYFERLKLKRLFCEPNAFNVAPNRALQKAGFKYLKTHMTVPGPLNYH
                     QAVTRWVIER"
     gene            complement(5692..>5893)
                     /locus_tag="VN968_13440"
     CDS             complement(5692..>5893)
                     /locus_tag="VN968_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXL30277.1"
                     /translation="KPGQAQPSFDKQPLRDYLDAERRDGRWNGEAPPPPLPASVVDAT
                     SKRYLEAYRRITGNDLAIGGAG"
BASE COUNT         1093 a         1814 c         1832 g         1154 t
ORIGIN      
        1 cgaacggcag cacctgcatc aggatcacgc cggtgacgtc gcgcgcggga tcgatccaga
       61 aataggtgtt ggcaaggccc gcccaggcga ggctgccggg gctgcggccc tccggcgtct
      121 tggcggtgtt gatcaggaag ctgagccccc atttcttgtc catgtccgga tagagatcga
      181 cgtggttggt gaagtccggc agcgccgaaa tcatcttggt catgttgagt tcgccgatgt
      241 ggttctgccc catcatcgcg acggtctccg gcctcagcag ctgattgccg ttgccgcggc
      301 ccttgttgag gatcatctgg gtgaacttga tataatcgcc cgcggtgctg tagaggccgc
      361 cgccgcccat gtggaattcc ggatcctgct cgagttcgaa tgggataggc gccagcgatc
      421 catccggccc gcgcgcatgc atgccgacca gtcgcttgcg ttgcgcgtcg ccgatcttga
      481 aggcggtgtc ggtcatgccg agcggcgcga acagatgatc gcgcagatag gcgtcgagcc
      541 gcttgccgct cgccgcttcc accgccttgc cgacgaaatc gatattgatg ccgtattccc
      601 atcgtgtgcc gggatcggac gtcagcggag tcttcagcgc agcgttctgg cagctgatga
      661 tgccgggcgt gccggtcttt gccatatact gcaccatgtc gccgttccac agatcatagc
      721 agaagccggc ggtatgggtc atgaggtggc gcagcgtgat cgggttcctg gccggccgca
      781 gcttcggctc gccgttggca tcaaaacctt cgagcacttg cggcgcggcg agatcgggca
      841 gcagttggcc gatcggggcc tccagggcca gccggccctg ctcgaccagt tgcatggcgc
      901 cggctgccgt gatcgccttg gtcatcgagg caatccagaa cacgctatcg gtggtcatgg
      961 cgtcgtcttt ggacaggtcg cgcttgccga acgcgccctg gtaggtcact tccgttgacg
     1021 tcgcggccac cgcgaccaag ccgggtattt ccgtggcgtc gcttttctga cgcagaacct
     1081 gatcgatctg agctttgctt tgcatggcgg acctcccgtt ttgatttttt gaagtcgtta
     1141 atcttcctcc cggccggctc cgcccgcaag cgcaggcagc gggctgagtt gcggcggtcg
     1201 cggcctggtg atggcggctg ccggggcggg gacgtgttta ccttaaggcg acgcggctgc
     1261 cacacttgcg acaatttctg gtggatttgc cgccattaat catgacctcc cgagatattc
     1321 ttggtagact cgaatcggtc acgggttttt gaatcgattt tcatagtaac gcgttcaggt
     1381 tgcagcataa actgacgctg ctggacagcg aatttaatca aattgatagc ggccttcgag
     1441 ccgcgaaggg accacaatga tctccacttg gagcgaatgg aaacggtatc cccgtgccgg
     1501 acgcggtgag aatatcgagg cgccgattac tcccggaatc tacgaagtcc gatatgccgg
     1561 taccggcgcc ctccactcgt tcgacgcggt cgataatgtc gcccaggcat tggcacagct
     1621 gccggtcggt tccaaatcgt ggttcgggcg gcgtggagcg caagccgcac ccgagctcga
     1681 atatcgcacc tgcgcgacct cctccaaggc agacgccaag gccgcggcgg agcgcatgat
     1741 tggccgccgt gacacctata tgagcggcgc ggcctgattt cagacttgtc attcccgggc
     1801 gatgccaacc ggcggcgcaa agtgccgacc cgttgggacc atcccggaat ggcgagcatt
     1861 agccccctgc ctctggaact ttggttggcc ctcaaaattg cttgaagccg ccaatcgctc
     1921 gcggccggtg cgtcgctgcc ggtttgagcc tatatttggc gtgaaggcca tctcaagaaa
     1981 acactccaag gagtaacgcc atgtccccga ccgctgccgc acgcccttcg caagcccccg
     2041 cgccttcgct gcgcgaccgt ctgaaggatg cttcgctgct gcgtgagcag tgttacatcg
     2101 acggcgcctg gaccggcacg cccgccaatc ccgtgaccaa cccggtcaac ggggtagagc
     2161 ttgcgaaggt gccgaaaatg aacacagcgg aagcaacgca agcggtcgaa gccgccgaac
     2221 gcgcgtttcc ggcctgggcc aaactcaccg ccaagcagcg ctccaatatc ttgcgcaaat
     2281 ggttcgacct gattatcgcc aaccgcgaag atctggcgct gatcctcact tcggagcagg
     2341 gcaagccgct ggccgaggca ctgggcgaag tcgacatcgg cggcgcctat gtcgagttct
     2401 tcgccgagga ggcgcggcgc gtctacggcg aaaccattcc gacgcagcgg ccggacgcgc
     2461 ggctcttggc gatccgccag ccgatcggcg tctgcggcgc gatcacgccg tggaattttc
     2521 cgtgttcgat gatcacccgc aaggtatcgc cggcgctggc cgccggctgc accgtagtcc
     2581 tgaagcccgc caatgagacg ccgcttaccg ccttggcgct ggtggcgctg gccgagaagg
     2641 ccggcgtgcc caagggcgtg ttcaatatcc tcaccggcaa ttcgtcggcg atcggcaagg
     2701 tgctgtgcga acatccggcg gtgcgctttg tcggttttac gggatcgacc gaggtcggaa
     2761 agattctgta tcagcaggcg gcggtcgggg tgaagaagct cgggctcgag cttggcggca
     2821 acgcgccgtt cgtggtgttc gacgacgccg acatcgatgc cgcggtggaa ggcgccatgg
     2881 tttcgaaata ccgcaacatg ggccagacct gcgtctgcgc caaccgcatc tatgcgcaag
     2941 acaagatcta cgatcagttc gtcgaaaaac tttccaggag agtcgcggca atgaagatcg
     3001 gcgacggcac cgagaccggc gtgacgcaag ggccgctgat caacatggcc gcggtcgaca
     3061 aggtcgaaaa gcacattgcg gacgccgtca agcacggcgc caagatcgtc accggcggaa
     3121 aacgccattc gcttggcggc agcttcttcg agcccacggt gctgtccaac gtcgcgcccg
     3181 acgcgctggt gtcgcaggaa gaaacgtttg ggccccttgc gccggtgttt cgtttcaagg
     3241 acgaggccga cgtgatcgcg atgtgcaaca actcgccatt cggtcttgcg tcctatttct
     3301 attcgcgcga tctcggccgg gtctggcggg tcgccgaagc gctcgaatcc ggcatggtcg
     3361 gggtcaacac gggcctgatc accaccgagg tggcgccgtt cggcggcgtc aaggaaagcg
     3421 gtctcggccg cgaaggctcg cgtcacggca tggatgaata tgtcgagatc aaatacgtga
     3481 tgatggcggg gatctaggcg cgtttagctt ccgcctccgg tacccgccga aaatccatcg
     3541 gcgggcaccc cttggccgtt ctctacgtca ccgacttacc agcgatcgcc gccgaccccg
     3601 acgccaacgc ttacgccagg cgcgcggaag ccgacgccgc cacgcggact gtcgtcccaa
     3661 tcacgatagt cgcggcgctc tatgtatcgc tcgcgcggcg cataatcgta ggagtctcgg
     3721 atgatcacgc gtcgttcgcc gcaggtgcga taggaggagc ggaatatgaa agaacgtctg
     3781 ttgattacca ccgcgatcgg cctgatgaag cggtggctat ggttcctgcc gttcgccggg
     3841 acgacccgag ttcattgatc cactgtgttc ccgccaaatg cgccgtgacg cccaacacca
     3901 gaggcaaagc gcgcggcgcc tgacagcgtt tcgccggacg ctatgacttc aagcccgccg
     3961 cgcatttcgt tggcgatggc gtcttcctct tcgagatccc attgccgcaa cgcggagagc
     4021 atgcaggttt gcgggaagcg cgcgatatcc ttcgccaatg caatcgcacg cgcgcgggtt
     4081 tcgccattgg gcacgagacg gttggcaagg cccatgcgca gcgcttcggc gccgccgacc
     4141 ggacgccccg tgagaatgag atcgatcgct tgcgagtgac cgatcagccg cggcagccgg
     4201 atggtgccga gatcgatcaa cggcacgccg aagcgccggc agaaaacgcc gaaagtcgca
     4261 tcttcggcgg cgacgcgcat gtcggcccac agcgccagtt ccatgccgcc ggcaaccgca
     4321 aaaccttcga tcgcggcgat caccggtttt gatagccgta accggctcgg tcccatcggc
     4381 gcgatcgaat cgtgaccgcc gatctcgcgc ttcttgttgg gatcgcccga ggccaccgcc
     4441 ttgaggtcgg cgccggcgca aaaagtgccg ccgacgccgg tgaacacggc aacggatgcc
     4501 tcggcatcgg catcgaaagc cctgaacgca tcgaacaatt ttcgcgcggt ggcgccatcc
     4561 acagcgttac ggcaatgcgg acggttgatg gagacgatgg tcaccggtcc atcgcgttcg
     4621 acgagcactg gatctgtgtc ggtcatgtct gcgctcctga ttgacgccaa cattcgaacg
     4681 gctatttgct gtcggagtca gcgtcagcgt tgggttgaag tagatcccac aggttaccat
     4741 agagatcctt gaagacggca accgtcccgt aactctcagc catgggctca cgaacaaaga
     4801 caacaccctt ggccttatac gagtgaaagt cgcgccagaa gtcatcggta tggagaaaca
     4861 ggaatatccg acctccggtt tgatttccga ttcgcgccga ctgttctttg ccggcagctc
     4921 gcgcaagaag caggcgtgtc tcggtcgagc ccggcggggc caccgtgacc cagcgcttgt
     4981 cttgcgccgc aatgtagatg tcttcgacca gagtgaatcc gagtatgcca atataataat
     5041 cgatggcttc gtcgtaatca cgtaccagga gggctacctg gccgatagtt tgccttcctg
     5101 tcatgcgtcg cccgctggct ccggtcatgc tcatcctcaa ccgcagtcaa tacgaactca
     5161 tcctccggtt cgcatgccct caccgttcga tcacccatcg ggtgacggct tgatgataat
     5221 tgagcgggcc cggcacggtc atgtgggtct tgagatactt gaagccggcc ttttgcagcg
     5281 cgcgattcgg tgccacgttg aacgcattcg gttcgcagaa aagccgcttc agcttcagcc
     5341 gctcgaaata gatgtcgacg ctgcgccgca cgccttcggc tccgatgccg cgactccgac
     5401 gctcgggatc aatgacgtgc aggtgcatgt gggcttgctc gccataggtg atcttgtcgg
     5461 aggtcgaaaa gccgacgggc tggtcgcccg acaaccagag gacggcaatc cttcctcgct
     5521 gctggatcgg ccgtgcgtaa tcttgccgga agcgttcgcg ccagatctcc ggatccggca
     5581 gacacgtggg atcgacgcca agcagttcca gatgttcggg ggtggactga agaaaatatt
     5641 cgatgatgat atccacttct ggaatcgtca tctccctgac cgtcagtgtc atcaaccagc
     5701 tcctccaatc gccagatcgt tcccggtgat acggcgatag gcctccagat aacgcttgct
     5761 ggtggcgtcc acgacgctgg ccggcaatgg cggcggcggg gcttcgccgt tccagcggcc
     5821 gtcgcggcgc tccgcgtcga gataatcgcg cagcggctgt ttgtcgaagc tcggttgggc
     5881 ttggccgggc ttg
//
LOCUS       DATNOI010000338         8007 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_935004,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000338 DATNOI010000000
VERSION     DATNOI010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935004"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2004
                     /locus_tag="VN968_13445"
     CDS             <1..2004
                     /locus_tag="VN968_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HXL30278.1"
                     /translation="FDDLRKEVEKWNPEEVERVSGVPGEQLKRVAKMFATEKPSTLIW
                     AMGQTQKTVGTANVRASCIALLMTGNVGRAGAGANIFRGHDNVQGATDVGLDIVTLPF
                     YYGLAEGAWKHWSRVWEVDYDYLQSRFDSKQIMETPGIPLTRWFDAVTLPKDQVAQKD
                     NVRAVFVQGHASNSITRIPESLNGLKALELLVIADPHPTTWASLAVEAGRKDGVYILP
                     VATQFECKGSRVASNRSLQWGEQIVKPVFEAKDDLEVIYLMAKKFGFADKMFKNIKVE
                     NNLPVAEDVLREMNRGSWSTGYCGQSPERLKAHMKNQAKFDMLTTRAPKDDPEVGGDY
                     YGLPWPCWGSPEVKHPGTPLLYNTNLGVMDGGGTFRPRFGIEREEKLPDGTTRKVSLL
                     ADGAYSKDSEIQDGYPEFTLASLKKLGWDKDLTEAEMATITKVNSANPDAVSWALDLS
                     GGIQRVALKHGCVPYGNGKARMNAFGLPDPIPVHREPIYTPRVDLVSKYPTLPDAKQF
                     RLPNIGFSVQKAAVDKGIAKQFPLILSSGRLVEYEGGGEETRTNPWLAELQQDMFIEI
                     SPADAADRGIKDGGWVWVTGAESNSKAKMKALVTERVGKGVAWMPFHFGGWFAGKDLR
                     GNYPQGTDPIVLGESANTITTYGYDPATGMQEPKVTLCQIAAA"
     gene            2017..2613
                     /gene="fdh3B"
                     /locus_tag="VN968_13450"
     CDS             2017..2613
                     /gene="fdh3B"
                     /locus_tag="VN968_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HXL30279.1"
                     /translation="MARMKFLCDADRCIECNACVTACKNEHEVPWGINRRRVVTINDG
                     KPGERSVSMACMHCTDAPCAAVCPVNCFYTTADAVVLHSKDLCIGCGYCFYACPFGAP
                     QYPKVGNFGSRGKMDKCTFCAGGPEADGSKEEYEKYGANRLAEGKLPLCAEMCSTKSL
                     LAGDGEIIAQIYKERVMKRGYGSGAWGWKTAYRETIES"
     gene            2655..3656
                     /locus_tag="VN968_13455"
     CDS             2655..3656
                     /locus_tag="VN968_13455"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HXL30280.1"
                     /translation="MASFARLIRVTVGACAFLLVIAASVPCNAQQVNPTASSVKEQQL
                     LQELNRVQGRVSIPDQRSGVLEQPAGRDWRGFRNVTLRWVGGIAILGMLALLVIFYLA
                     RGMVRLESGRSGRTLVRFSAFERFVHWMTASCFIVLAISGLNITFGRPLLLPLIGFAA
                     FSEWSQWAKYAHNYLSFPFTIGVVLIFLLWLAGNIPNKVDVAWLKRGGGIVGHDHPPA
                     YRFNAGQKAIYWIVVIGGGAVAVTGYQLMFPFYLSGIEGMQIAQIVHSIVSVLFIAVM
                     LAHIYIGTIGMEGAFEAMGTGEVDLNWAKEHHPLWVEQELAKGHAPPGSAQRPVAAE"
     gene            3689..3847
                     /locus_tag="VN968_13460"
     CDS             3689..3847
                     /locus_tag="VN968_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30281.1"
                     /translation="MKAFVAALLFAAIAAVGLSMLLNTIQEPSSVAFTTGAARVGDPG
                     HNLVGPNG"
     gene            3897..4298
                     /locus_tag="VN968_13465"
     CDS             3897..4298
                     /locus_tag="VN968_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020372397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HXL30282.1"
                     /translation="MSAPNASVPNATAAWPVEIRLAKDRQTLHVAFDDGKAFSLSAEL
                     LRVTSPSAEVQGHSEAQRKTVGGKRNVTILSVDPVGNYAVRLGFDDLHTTGIYSWAFL
                     RDLGENAERRFQDYLDDLQAKGLDRDKPGMR"
     gene            4440..5678
                     /locus_tag="VN968_13470"
     CDS             4440..5678
                     /locus_tag="VN968_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXL30283.1"
                     /translation="MTGALAQLRGHGGPVRALAISSDGQTAVTGSFDSTAIRWSLARN
                     AAEQVLRFHADAINAVVLLRDGRTATAGADGRIAIWTPGKAEPDAVLEGHTAPIAALA
                     ASPDGATLASASWDQTVLLWPLAGGAARVLEGHTQNVNGVAFAPDGRTLVSVSYDQSI
                     RIWPLSSASAPTVVAMPSPLNAVAIGGDGEIVAGGADGKLYFLSGTGVAAGEIAAGPK
                     PVISIAISPDGALVAAASIGGSVAVVDRKTRDLARILVGPGLPVWSVAFLPDSRTLLT
                     GGADNIIRRWDAVTGDSIDPLVLEMAGDPLAAYAGDHGAEVFRACVACHTLNAEQANK
                     AGPTLAGIFGRRIATLPGYNFSEALKRLDIVWTPETVSKLFEIGPSAYTPGTKMPEQH
                     IGSEQDRAALVQFLERATRK"
     gene            complement(5685..6515)
                     /locus_tag="VN968_13475"
     CDS             complement(5685..6515)
                     /locus_tag="VN968_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXL30284.1"
                     /translation="MPNRFLSFALALCVSAAACAPAIAEDRSIIVASTTSTQDSGLFG
                     YLLPIFKAKTGIEVKVIAQGTGQALDTARRGDADVVFVHAKPQEEKFLAEGFGVKRFD
                     VMYNDFVLIGPKDDPAGVKGKDIETALKTIQAKDAPFVSRGDRSGTHAAELALWKQAG
                     IDIAAGKGPWYREIGQGMGAALNTASAMNGYVLSDRGTWISFKNRGDLQIVVEGDKRL
                     FNQYGVMLVNPEKYPSVKKELGQAFVDWLVSPEGRAAIAGYKIDGQQLFFPDAEKKGS
                     "
     gene            complement(6535..7257)
                     /locus_tag="VN968_13480"
     CDS             complement(6535..7257)
                     /locus_tag="VN968_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30285.1"
                     /translation="MRAPKSDLPLVLDDVSLQAGATTILNRLSLRINPGAPTLIVGPN
                     GAGKTTLLRLCMGLTAPSSGRIAWGGRADSALTRRAILFQRPVMLRRTTAANVAYALA
                     QAGTPRQLRAQRAAALLDRVGLSDLAQRPARRLSGGEQQRLALARALARDPEILLLDE
                     PTANLDPAATRSVEEIVLMAAQSGIKIVMASHDLGQVRRLAGDVIFLVRGALCELGRA
                     DDFLDRPTTPEAAAFVRGDLVI"
     gene            complement(7260..7952)
                     /locus_tag="VN968_13485"
     CDS             complement(7260..7952)
                     /locus_tag="VN968_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL30286.1"
                     /translation="MSDQFSAWQLILNGDPTLVAIVRLSLAVSLSAVMLAALVGMPLG
                     ALLALTRFPGRSFLVVLLNALMGLPPVVVGLAVYLLLSRSGPLGAMGILFTPSAMVIA
                     QTILIVPIIAALTRQTVEDLWVEYRDELSAMDVAPIGRMTTLLWDARFSLLTALLAGF
                     GRAAAEVSAVMIVGGNIDGFTRTMTTAVALETSKGNLPLAIGLGLVLVVIVLAINAAA
                     WGTRVWSERQAG"
BASE COUNT         1522 a         2522 c         2508 g         1455 t
ORIGIN      
        1 ttcgacgatc tgcgcaagga agtggagaag tggaatccgg aagaagtcga gcgcgtcagt
       61 ggcgttcctg gcgaacagct taagcgcgtc gccaagatgt ttgccacgga gaagccgtca
      121 acgctgatct gggccatggg ccagacccag aaaaccgttg gcaccgccaa cgtgcgggcg
      181 agttgcatcg cactgttgat gaccggcaat gtcggcaggg ccggcgccgg cgccaacatc
      241 ttccgtggcc atgacaacgt gcaaggagcg accgacgtcg ggctcgatat cgtcacgctg
      301 ccgttctatt acgggctcgc cgaaggcgcc tggaaacact ggtcgcgggt ttgggaggtc
      361 gactacgatt atctgcagtc gcgcttcgac tcaaagcaga tcatggaaac ccccggcatc
      421 ccgctcaccc gctggttcga cgcggtgacg ctgccgaaag accaggtcgc gcagaaagac
      481 aacgtgcggg cggtattcgt gcagggccat gccagcaaca gcatcacccg cattccggaa
      541 tccctgaacg gtctaaaggc gctcgaactg ctggtcatcg ccgacccgca tccgaccacc
      601 tgggcctcgc tcgccgtgga ggccgggcgc aaggatggcg tctacattct cccggttgcc
      661 acgcaattcg agtgcaaggg ttcgcgcgtt gcctcgaacc gctcgctgca atggggcgag
      721 cagatcgtaa agccggtgtt cgaagcgaag gatgacctcg aggtcatcta tctgatggcg
      781 aagaagttcg gattcgccga caagatgttc aagaacatca aggtcgagaa caatctgccg
      841 gtcgcggagg atgtgcttcg ggaaatgaac cgcggcagct ggtctaccgg ctattgcggg
      901 caatcgccgg agcggctcaa ggcgcacatg aagaaccagg cgaagttcga catgttgacg
      961 acgcgggcgc ccaaggacga tcctgaagtc ggcggcgact attacggcct gccctggccg
     1021 tgctggggct caccggaagt caagcatccg ggtacgccgc tgctctacaa tacaaatctc
     1081 ggcgtgatgg acggcggcgg cacgttccgg ccgcgtttcg gaatcgagcg cgaggagaag
     1141 cttcccgacg gcacgacgcg aaaggtcagt ttgctggcgg atggcgccta ctccaaggat
     1201 tcggaaatcc aggacggcta tccggaattc acgctggcga gcctgaagaa gctcggctgg
     1261 gacaaggatc tgacggaagc cgaaatggcc accatcacca aggtcaattc cgccaatccc
     1321 gatgcggtgt cgtgggcgct cgacctgtcg ggcgggatcc agcgcgtggc gctcaagcat
     1381 ggttgcgttc cctacggcaa cggcaaggcg cgcatgaatg cgttcggctt gcccgatccg
     1441 attccggttc accgcgagcc gatctacacg ccgcgggtcg atctggtctc gaaatatccg
     1501 acgctgcccg acgccaagca gttccgcctg cccaacatcg gcttctccgt gcagaaggcc
     1561 gccgtggaca aggggattgc caagcaattc cccctcatcc tctcgtcggg ccgtctcgtc
     1621 gaatatgagg gcggtggcga agagacccgt accaatccct ggctcgcaga actgcagcag
     1681 gacatgttca tcgagatcag cccggccgat gccgccgatc gcggcatcaa ggacggcggc
     1741 tgggtctggg tcacgggtgc cgagagcaat tccaaggcca agatgaaggc gctcgtcacc
     1801 gagcgtgtcg gcaaaggagt cgcctggatg cccttccact tcggcggctg gttcgcaggc
     1861 aaagatcttc gcggcaacta cccgcagggc accgacccga tcgttctcgg cgaaagcgcg
     1921 aataccatca ccacctacgg atacgatccg gcgaccggca tgcaggagcc caaagttact
     1981 ctctgccaaa tcgcggcggc ataaggagca acgaccatgg cacgtatgaa attcctctgc
     2041 gacgccgacc gttgcatcga atgcaatgcc tgcgtgacag cctgcaagaa cgagcacgag
     2101 gtgccctggg gcatcaatcg ccgccgcgtt gtcaccatca atgacggcaa gcccggcgaa
     2161 cgctcggtgt cgatggcctg catgcattgc accgacgcgc cctgcgcggc ggtttgcccg
     2221 gtgaactgct tctacacgac ggccgacgct gtcgtgctcc actccaagga cctgtgcatc
     2281 ggctgcggtt actgcttcta cgcctgtccg ttcggcgcgc cgcaatatcc gaaggttggc
     2341 aacttcggtt cacgcggcaa gatggacaag tgcaccttct gcgctggtgg tccagaggcc
     2401 gacggcagca aggaggaata cgagaagtat ggcgcgaacc gtctcgccga gggcaagctg
     2461 ccgctatgcg ccgagatgtg ctcgaccaag tcgttgctcg ccggcgacgg cgaaatcatc
     2521 gcccagatct acaaggaacg cgtgatgaag cgcggctatg gctcaggtgc ctggggttgg
     2581 aagaccgcgt atcgcgaaac gatcgaatcc taagcgcgca tggttgcgat cgctgcaata
     2641 ttccggagga gttcatggcg tcttttgcac gattgatccg cgtcactgtc ggcgcctgtg
     2701 cgttcttgct ggtcatcgcc gcatcggtgc cctgcaacgc ccagcaggtt aatccgacgg
     2761 caagctccgt gaaggaacag cagctcctgc aggagctcaa tcgagttcag ggacgcgtca
     2821 gcattcccga tcagcgctct ggcgttctcg aacagccggc gggccgtgac tggcgggggt
     2881 tccgcaatgt cacgctgcgc tgggttggcg ggatcgccat cctcggcatg ctcgccctgc
     2941 tcgtcatctt ttatcttgcg cgcggcatgg tccggctcga gagcgggcgg tccggccgta
     3001 ctcttgttcg cttctcggca ttcgagcgct tcgtgcactg gatgacggcg agctgcttca
     3061 tcgttctggc gatctcggga ttgaatatca ctttcggccg gccgctgctg ttgccgctga
     3121 tcggcttcgc ggcgttctcc gagtggtcgc aatgggcgaa atatgcgcat aactatctga
     3181 gcttcccctt caccatcggc gtcgtgctga tcttcctgct gtggctcgcc ggcaatattc
     3241 caaacaaggt cgatgtcgct tggctcaaaa ggggtggcgg aatcgtcggt catgaccatc
     3301 cgccagccta ccgctttaat gcaggtcaga aggcgatcta ctggatcgtc gtcatcggtg
     3361 gcggggctgt tgccgtcacc ggctatcagc tgatgttccc gttctacctt tccggcattg
     3421 aaggaatgca gattgcccag atcgttcact cgatcgtgtc ggttctcttc attgccgtca
     3481 tgctggcgca catctacatc ggcaccatcg gcatggaggg cgcattcgag gcgatgggga
     3541 ccggcgaggt cgatctcaac tgggccaagg agcatcaccc gctctgggtc gagcaagagc
     3601 tggccaaggg ccacgcgccg ccgggaagcg cacagcgtcc cgtcgcagct gaataattcg
     3661 gtttgaacaa gtccatctgg ggagtgtcat gaaagccttt gttgcggcct tgttgtttgc
     3721 agcgattgca gccgtgggcc tgtcgatgtt gctcaatacc atccaggagc cgagctccgt
     3781 cgcattcacc accggcgccg ccagggtcgg cgatcccggc cacaacctgg tcgggccaaa
     3841 cggatagagg tttgtaggta tcgggcaggc gccagatcga gacacaggac tgtccattga
     3901 gcgcacccaa tgccagcgta cccaatgcca ccgcggcttg gccggtagaa atccggctcg
     3961 ccaaggaccg ccaaaccctg catgtcgcat ttgacgatgg caaggcattc agcctctccg
     4021 ccgaactgct tcgcgtcacc agcccctccg ccgaagtgca gggccactcc gaagcgcaac
     4081 gcaagacggt cggcggcaag cgcaacgtga cgattctctc ggtcgatccg gtcggcaatt
     4141 atgcagtgcg gctcgggttc gacgacttgc acaccaccgg catctattcc tgggcgtttc
     4201 tgcgcgatct cggggagaac gccgagcggc gctttcagga ttacctcgac gatctccagg
     4261 ccaagggcct cgaccgcgac aaaccgggca tgcgctaacg cgggttcgga tcgcgcgcct
     4321 cgcgcgttat cactgatgtt gatcgtctga acaaacgatt gccgcgaatg ctccttgccc
     4381 ggaaaaatcg ccctgcactt ccgacctgtg ctgccttgct ggtaattctg ccgctgggga
     4441 tgacgggcgc gctcgcgcag ttgcgcggcc atggcgggcc agtgcgggcg ctggcgatct
     4501 cttccgacgg acaaactgct gttaccggca gctttgattc aaccgcgatc cgctggtcgc
     4561 tggcgcgcaa tgcggccgaa caggtgctcc gcttccatgc cgatgccatc aacgcggtgg
     4621 tgctgctcag ggatggacgc acggcaaccg caggcgccga cgggcgcata gcgatctgga
     4681 ctcccggcaa ggccgagccc gatgccgtgc tggaagggca tacagcgccg atcgcggcgc
     4741 tggctgcatc gcccgacggt gcaacattgg cgtccgcgtc gtgggatcaa acggttctgc
     4801 tgtggccgct tgcaggcggc gcggcgcggg tgctcgaagg gcatacgcag aacgtcaatg
     4861 gcgtggcgtt cgctcccgac ggccgcacgc tggtcagcgt cagctacgat cagagcattc
     4921 gcatttggcc gctttcgagc gcctccgctc cgaccgtcgt tgcgatgccg agcccgctca
     4981 acgccgtcgc catcggcggc gatggcgaaa tcgtcgccgg tggcgccgac ggcaaacttt
     5041 attttctgag cggaaccggc gtggccgcag gcgagatcgc agccgggcca aaaccggtga
     5101 tctcgattgc gatctcgccg gacggcgcgc tagtcgcggc cgcgagcatt ggcggatcgg
     5161 tggcggtggt cgaccgcaag acgcgcgatc tggcgcgcat attggtcggg cccgggctgc
     5221 cggtctggtc ggtggcattt ttgccggaca gccgcaccct gctcaccggc ggcgccgaca
     5281 acatcatccg acgctgggat gcagtgacgg gagatagtat cgatccgctt gttctggaga
     5341 tggcgggaga tccgctcgcc gcctatgccg gcgaccacgg cgctgaagtg tttcgcgcct
     5401 gtgtggcctg ccatacgctt aacgccgagc aggccaacaa agcgggcccg acgctggccg
     5461 gcattttcgg ccggcggatc gcaaccctgc cgggctataa tttttcggaa gcgctgaagc
     5521 gcctcgatat cgtgtggacg ccggagacgg tgtcgaaatt gttcgagatc ggaccttccg
     5581 cctacactcc cggcaccaag atgcccgagc agcacatcgg ttcggaacag gaccgcgccg
     5641 cgctggtgca gttcctcgag cgcgcgacca gaaaatagcc gggttcaaga cccctttttc
     5701 tccgcatcgg gaaagaacag ctgctggcca tcgatcttgt agccggcgat cgcggcccgg
     5761 ccttcgggcg agacgagcca atcgacaaac gcctggccga gctctttctt cacggaggga
     5821 tatttttccg ggttcaccag catgacgccg tattgattga acagccgctt gtcgccttcg
     5881 accacgattt gaagatcgcc gcggttcttg aacgatatcc aggtgccgcg gtcggacagc
     5941 acatagccgt tcatcgcgct cgcggtattg agcgcggccc ccatgccttg cccgatctcg
     6001 cgataccacg ggcctttgcc ggccgcgata tcgataccgg cctgtttcca gagtgccagc
     6061 tcagccgcat gcgtgcccga tctgtcgccg cgcgagacga agggcgcatc ttttgcctgg
     6121 atcgttttca gcgcggtctc gatgtccttg cccttgacgc cggcgggatc gtcctttggt
     6181 ccgatcagca cgaaatcgtt gtacatcacg tcaaaccgct tcacgccaaa gccttcggcg
     6241 aggaatttct cttcctgcgg tttggcatgg acgaacacca cgtccgcgtc gccgcgtcgc
     6301 gcggtgtcga gcgcctggcc ggtgccctgc gcgatcacct tcacttcgat gccggttttg
     6361 gctttgaaga tcggcaagag atagccgaac agcccggaat cctgcgtcga cgtggtcgac
     6421 gccacgatga tcgatcgatc ctcggcgatt gcgggtgcgc aagcggcggc gcttacgcac
     6481 agcgccaacg cgaaagacaa gaaacgattc ggcatgatca actccagttc agattcaaat
     6541 cacgagatcg ccgcggacga aggcggcggc ttccggtgtc gttgggcgat cgagaaaatc
     6601 atccgcacgt cccagctcgc acagtgcgcc gcggaccaga aaaatcacgt cgccagcgag
     6661 ccggcggacc tggccgagat cgtgcgaggc catcacgatc ttgatgccgg actgcgctgc
     6721 catcagcacg atctcctcga cgctgcgtgt ggcggccgga tcgagattgg cggtgggctc
     6781 gtcgagcaac aggatttccg gatcgcgcgc cagcgcccgc gccagtgcca gccgctgctg
     6841 ttcaccgccc gagagccgcc gcgccggccg ctgcgccaga tcggagagcc cgacgcggtc
     6901 cagcaatgcc gcagcgcgtt gcgcgcgcag ctggcgcggc gtgccggcct gggccagcgc
     6961 ataggcgacg ttggcggctg tggtccttcg caacatcacc gggcgttgaa acaggatcgc
     7021 gcggcgcgtc agcgcgctat cggcccggcc gccccaagcg atgcggccgc tcgatggcgc
     7081 tgtaagcccc atgcagagcc gcagcaatgt cgtcttcccc gcgccattgg ggccgacgat
     7141 cagcgtcggc gcgcccggat tgatcctcag gctcaaacgg ttgaggatcg tggtggcgcc
     7201 tgcctgcagc gagacgtcgt ccagaaccaa aggaagatcg ctcttgggtg cccgcatcgt
     7261 cagcccgcct gccgctcgga ccacacccgc gtgccccacg ccgcggcgtt gatcgcaaga
     7321 acaatgacga caagaacgag cccgagccct atcgccagcg gcagattgcc cttggaggtt
     7381 tctagcgcga ccgccgtcgt catggtgcgg gtgaagccat cgatattgcc accgacgatc
     7441 atcacggcgc tgacctcggc ggcggcccgc ccaaaacctg ccaatagcgc ggtaagcagg
     7501 ctaaagcgcg catcccacaa tagagtcgtc atgcggccga tcggcgcgac atccatcgcg
     7561 ctcagttcgt cgcgatactc aacccagaga tcctcaacgg tctggcgcgt cagcgcggca
     7621 atgatcggca cgatcaaaat ggtctgggcg atcaccatgg ctgatggcgt gaacagaatg
     7681 cccatcgcgc ccagcggccc cgagcgtgac agcaagagat aaaccgcaag cccgaccacc
     7741 accggcggca atcccatcag cgcattgagc agcacgacca aaaaggagcg tccgggaaaa
     7801 cgggtcagcg cgagcagcgc acccaggggc atgccgacga gcgccgccag catcaccgcg
     7861 gacaggctaa cggcgagcga tagccgcacg atcgcaacca gcgtcggatc gccgttgagg
     7921 atcaattgcc aggcgctgaa ctggtcggac atcgccatct cccgcccttg ttcggcaaga
     7981 attccatcat ttgtcaaatg gttgaat
//
LOCUS       DATNOI010000339         9214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1096874,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000339 DATNOI010000000
VERSION     DATNOI010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9214
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096874"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(397..1731)
                     /locus_tag="VN968_13490"
     CDS             complement(397..1731)
                     /locus_tag="VN968_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXL30287.1"
                     /translation="MRKNSLFLLGAATGVGLTLLVTGPQGSQLVAAAMAATGPDTYSQ
                     LNLFGEVFERIRTNYVEKPDDAKLIEASINGMITALDPHSRYMNEKAWRDMRETTSGE
                     FGGLGIEVMMEDGLVKVVAPMDDTPAAKAGIMSGDLITHINGEAVEGLTLEQAVSKMK
                     GPISTKTRLKIIRKGTEAPIEVSIMREIIRMRPVSYHTDGGDIGYVKITTFNEQTTDG
                     LKKAIADISKQIPPEKLAGYVVDLRNNPGGLLDQAVSVSGTFMARGEIVSTRGRTAEE
                     TQRFAARTGDLIKGKPLVVLINGGSASASEIVAGALHDHKRATLIGTRSFGKGSVQTI
                     IPLGTGNGALALTTARYFTPSGHSIQAQGVVPDIEVLQDVPDEMKARFDMKGEASMRG
                     HLSAEGAEQSGSQSYVPPVEKDDKALGAAYNLLRGVTVNAGVPSSPKAAVPN"
     gene            2129..3262
                     /locus_tag="VN968_13495"
     CDS             2129..3262
                     /locus_tag="VN968_13495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HXL30288.1"
                     /translation="MNEATRIRPGTAAELGASGEEFQNLHEFVRKARANLNQNAWDYI
                     VGAAETETTMRRNRMALDEIAFRPRVLRNVAKVDASVEVFGRKLRLPVMLAPVGALEI
                     FDPGAAAAVARGAGAFGAAHMLSSVSEPGLEKVAEAAPGALRIFQLYVRGDDGFVEDH
                     VARAISGGYTAFCLTVDTAHYSRRERDIAKRYVRASRARSTGGDFQKGLEWRTVKLIK
                     DKYKIPLIIKGIATAEDAHIALDHGVDWIYVSNHGGRQLDHGRGSMHVLPEIVNAVAG
                     RAKIMVDGSICRGTDIVKAIVAGADLVGIGRLQCWALAAKGEAGVVRMLELLEDEVIR
                     SLGLLGVTRFAELDKSYLHPAAPTNLPHVLSAFPLLEIEPYRY"
     gene            3267..3899
                     /locus_tag="VN968_13500"
     CDS             3267..3899
                     /locus_tag="VN968_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXL30289.1"
                     /translation="MTPPLLSPGAADALLFDLGRVVLDIDFSKVVACWAGHAGCEPAH
                     LVGRFSWRDEFYQQHEKGEISDAEFFAALRALLGVELSDAQFLEGWNEIFAGEMPGIA
                     PLLQRAARRLPLYAFSNTNAAHVEHFSETYAGVLGHFREIFLSSAIGLRKPDAAAYDH
                     VVKAIGVPASRIVFFDDLAENIDGARACGLTAVHVTSPDDVAHALAALGI"
     gene            3925..5136
                     /gene="glp"
                     /locus_tag="VN968_13505"
     CDS             3925..5136
                     /gene="glp"
                     /locus_tag="VN968_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HXL30290.1"
                     /translation="MALMPVTEALSAVLAGAEPLPEEMVPLDAAHHRVLAHDVAALRT
                     QPPQAMSAMDGYAVRSADAAHVAARLKVIGEVAAGRPFDTSVGAGEAVRIFTGGVIPQ
                     GADAVIIQEDTVAEAGGITITEAARPGRHIRPAGVDFREGDVLLKGGIRLTDRDLSLA
                     AGMNYPELAVRRRPKVAVLATGDELVMPGSTPGPGQIVYSNGYALRALARAEGAETID
                     LGIAADTVEATTEGIRRARDSGADILITTGGASVGDHDLVKQSLEAEGVTMAFWRIAM
                     RPGKPMMHGRLGAMRVIGLPGNPVSSYVCGFLFLVPLIRCLSGRTGIHHAHETALLGR
                     DVAANDLREDYLRARLEMRSDGVLIATPVDHQDSSLLGNLAAARALVIRAPFAAAAAA
                     ESVCDILRLPG"
     gene            5353..6063
                     /gene="lexA"
                     /locus_tag="VN968_13510"
     CDS             5353..6063
                     /gene="lexA"
                     /locus_tag="VN968_13510"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HXL30291.1"
                     /translation="MLTRKQYELLRFISERLKESGVPPSFDEMKDALDLRSKSGIHRL
                     ITALEERGFIRRLPNRARAIEVIKLPELSGSSNNGNGRRGFTPSVIEGTLGRVRSSSV
                     GSEDDNNRPVAVPVMGRIAAGTPIEALQTRSHTISVPPDMLGSGEHYALEVRGDSMVD
                     AGILDGDMALIQRNETAETGDIVVALIDEEEATLKRFRRRGASIALEPANTSYEVRIL
                     PPNRVKIQGKLIGLYRKY"
     gene            6450..6872
                     /locus_tag="VN968_13515"
     CDS             6450..6872
                     /locus_tag="VN968_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30292.1"
                     /translation="MCDYSLHAVATRPARVGETLITTTFRGTSTRGFASESEPAVAVC
                     MLPGTELAFAQNVKYDNRWIWTKTLNFRVGKFGAIEPDVAHRHHDAIEFPDGSHVLVT
                     QLCEGQRATVLQLPVTDRVAGHAPSVANTNAATPVSVD"
     gene            7082..7339
                     /locus_tag="VN968_13520"
     CDS             7082..7339
                     /locus_tag="VN968_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30293.1"
                     /translation="MRTSRVAHGPGARAVKKVTWRQVGRVTEPGRYMFRFGWLTIAAE
                     DLAVWEQLPNAAFTLVRTAAEIEEYRLGAFELREDLALREK"
     gene            8096..8437
                     /locus_tag="VN968_13525"
     CDS             8096..8437
                     /locus_tag="VN968_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30294.1"
                     /translation="MTAVAEALSREFPATTSRQPNTNIDVLLSANEMERLGTNVKEHL
                     ILGTDKQDAEQQRDLWLSENPSIRVIKIHRVKREPQTLLTLIGSKHVPRVSILVEYEE
                     TEVSAANVAAE"
     gene            complement(8445..8642)
                     /locus_tag="VN968_13530"
     CDS             complement(8445..8642)
                     /locus_tag="VN968_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30295.1"
                     /translation="MDPTKLRMRYLLGIFLAAVVTAAVLRHVLHTQVGIAREDIRTGV
                     LIAAAVGISLLIAFFRWSRRQ"
     gene            complement(8742..>9214)
                     /locus_tag="VN968_13535"
     CDS             complement(8742..>9214)
                     /locus_tag="VN968_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="competence protein ComEC"
                     /protein_id="HXL30296.1"
                     /translation="HLMRTAKDVFLLKEWLAADADPRQPTDASLAEGVSCDEAGCVAQ
                     TAAGGLITLALRPDALADDCERAALVVTMRQAPPSCASPVIDRDRLRRLGAMALRRTA
                     TGFAVDAVKPKGVDRPWSPAMAGDIEAETNLMTRPTAPRAVDATPSEADLQAED"
BASE COUNT         1715 a         2934 c         2830 g         1735 t
ORIGIN      
        1 gggctacctg ttcagtccgg caaagccggc cgcggagatc aggcagctat tccaccgcga
       61 acaatcagcg ggcgcggacc tcaggccgcg tcgaaagcga cggcaaatgc cgaccaccgc
      121 gtgaggtcca gagcgttttc agtggagacc ggttcgcgtc aagaaaacgc gtcaaatcaa
      181 gaatctagag ccccgtttcg attttgtcga aacggggctc tagtacctgg aatcacaaat
      241 cggtattgcg gcccatcctt cgagacggcc acttcgtgac ctcctcagga tgaggtctaa
      301 ctcgttgaag cacaacaacc tcatgctgag gagcgagcga cgcgagcgtc tcgaagcatg
      361 ggcagcaagc gactcacaga tctaacatag tcagtactag ttcggaaccg ccgccttggg
      421 agatgaaggc acacccgcat tgaccgtgac gccgcgcagg aggttgtagg ccgcgccaag
      481 cgccttgtcg tccttctcca cgggcggaac gtaagactgc gagccggact gctcggcacc
      541 ctcggcggaa agatggccgc gcatggacgc ctcgcccttc atatcgaagc gtgccttcat
      601 ctcgtccggc acatcctgca gcacttcgat atcggggacg acgccctgcg cctggatcga
      661 atggccggac ggcgtaaaat aacgcgcggt ggtgagtgcc aacgcaccgt tgccggtgcc
      721 gagcggaata atcgtctgta ccgagccctt gccgaacgag cgggtcccga tgagggtcgc
      781 gcgcttgtga tcgtgcaagg caccggccac gatctccgaa gccgatgccg atccgccgtt
      841 gatcaacacg acaagcggct tgcccttgat gagatcgccg gttcgcgccg cgaagcgctg
      901 ggtctcctcc gcggttcggc ctcgcgtcga gacaatctcg ccccgcgcca tgaacgtgcc
      961 ggacacggag accgcctgat cgagcagtcc gccgggatta ttgcgcaggt cgaccacgta
     1021 gccagccaac ttttccggcg ggatctgttt cgaaatgtcg gcgatcgctt ttttcaatcc
     1081 gtcggttgtc tgttcgttga aggtggtgat cttgacatag ccgatatcgc caccgtcggt
     1141 gtggtagctg accgggcgca tgcgaatgat ttcgcgcata atcgaaactt cgatcggtgc
     1201 ctccgtgccc ttgcggatga tcttgagtcg ggtcttcgtg ctgatcggcc ccttcatctt
     1261 gctcacagcc tgctccaggg tgagcccttc cactgcttcg ccattgatgt gggtgatcag
     1321 gtcgcctgac atgatgccgg ccttcgccgc gggcgtatca tccatcggcg caaccacctt
     1381 gacgaggcca tcctccatca tgacctcaat ccccagcccg ccgaactcgc cgctggttgt
     1441 ctcccgcatg tcccgccagg ccttctcgtt catgtaccgc gagtggggat cgagcgccgt
     1501 gatcatgcca ttgatcgacg cctcgataag tttggcgtcg tcgggctttt caacatagtt
     1561 cgtccgtatc cgctcgaaca cctcgccaaa caggttgagc tgggaatagg tgtcggggcc
     1621 ggtcgcagcc atggccgccg caaccaattg cgagccctga ggcccggtga ccaggagggt
     1681 tagacccaca cccgtagccg caccgaggag aaagagggag ttcttgcgca ttgttcgcga
     1741 accttcctgc tgtcagttgc tgcccgccac ggggtggtta aatcggtgtc ctgatctgcg
     1801 ccggccgtct cacgatatac gcgctccgcg accgcggcaa agccggaaat cgctcgtttc
     1861 acatcgattt gcaattgccc cgggttcgaa tcgcaacccc acttcatcat gaccccgtgc
     1921 ccgccggccg ttggctgcgc agtaggagga tgcggacgaa atgcggccgc gaagctgttg
     1981 gtgccggcag gaacgcgccc actcaggatc ggagagacgt tgccgccgct gataccagcc
     2041 ataccaattt ttgcgatggc gccgaggctc aggaagctat acccgaaagg ttaaccaacg
     2101 aactcacgca ttcctgggga ggacagtcat gaacgaagcg acccgcatcc ggccggggac
     2161 ggccgccgaa ctcggcgcga gcggcgaaga attccagaac ctgcacgagt tcgtacggaa
     2221 agcccgcgcc aacctcaacc agaatgcctg ggactacatt gtcggcgccg ccgagaccga
     2281 gaccaccatg cgccgcaacc gcatggcact cgacgagatt gcattccggc cgcgggtgct
     2341 gcgcaatgtc gccaaggtcg acgcgtccgt cgaggtattc ggccgcaaat tgcgcctgcc
     2401 tgtgatgctc gcaccggtcg gcgcgctcga gattttcgat ccgggcgcag ccgcagccgt
     2461 cgcgcgcggc gctggcgcgt tcggcgccgc ccatatgctg agctcggtct ccgagcccgg
     2521 cctcgagaaa gtcgccgagg ctgcgcccgg cgcgctgcgc atttttcagc tctacgttcg
     2581 cggcgatgac ggctttgtcg aggaccatgt cgcgcgtgcg atatcaggcg gatataccgc
     2641 gttctgtctc accgtcgaca ccgcgcatta cagccggcgc gagcgcgaca tcgccaagcg
     2701 ctatgtccgc gccagccgcg cccgctccac cgggggcgac ttccagaagg ggctggaatg
     2761 gcgcaccgtg aaactgatca aggacaagta caagatcccg ctgatcatca agggcattgc
     2821 gaccgcggaa gatgcgcaca tcgcgctcga tcacggcgtg gattggatct acgtgtcgaa
     2881 ccacggtgga cgccagctcg accatggccg cggctcgatg catgtactgc ctgaaatcgt
     2941 caacgccgtc gccggccgcg ccaagatcat ggtcgatggc tcgatctgcc gcggcaccga
     3001 catcgtcaag gcgattgtag ccggcgctga tcttgtcggc atcggccggc tgcaatgctg
     3061 ggcgctcgcc gccaagggcg aggccggagt agtgcggatg ctggaactgc tcgaggacga
     3121 ggtgatcagg tcgctggggc tgcttggcgt cacgcgtttc gccgaactcg acaagtccta
     3181 tctgcatccg gcggcgccga ccaacctgcc ccatgtcctc agcgccttcc cgctcctgga
     3241 gatcgagcct tatcgttatt gaccccatga ccccacccct gctctccccc ggcgccgccg
     3301 acgcgctgtt gttcgatctc ggccgcgtgg tgctcgacat tgatttcagc aaggttgtcg
     3361 cctgctgggc cggtcacgcc ggctgcgagc ccgcgcatct cgtggggcga ttttcctggc
     3421 gcgacgaatt ctatcagcag cacgagaagg gcgagatcag cgacgccgaa ttttttgccg
     3481 cactccgcgc cctgctcggc gtcgagctgt ctgacgccca gttcctggaa ggatggaacg
     3541 agatcttcgc cggcgaaatg ccgggcatcg cgccgctgct tcagcgcgcc gcgcggcgcc
     3601 tgccgctcta cgccttctcc aacaccaacg cagcccatgt cgaacatttt tcggaaacct
     3661 atgccggggt gcttggccat ttccgcgaaa tctttctgtc gtctgcgatc gggcttcgga
     3721 aaccggatgc cgcggcctat gatcatgtgg tgaaagcgat cggcgtgccc gcctcccgta
     3781 tcgtgttctt cgatgatctc gccgagaaca tcgacggtgc gcgggcgtgt ggcctcaccg
     3841 ccgttcacgt gacatcgccc gacgacgtgg cgcatgcact ggccgcgctt gggatctagg
     3901 agttttggct ttgggagttt tatcttggcc ttgatgccgg ttactgaggc gctgtcagcg
     3961 gttctcgcgg gcgccgagcc gctgcccgaa gaaatggtcc cgctcgatgc ggctcatcat
     4021 cgggtgctcg cgcacgacgt cgcggcattg cgcacccagc cgccgcaggc gatgtcggcg
     4081 atggacggct atgcggtccg gtccgccgat gctgcccatg tggcggcgcg gctcaaagtg
     4141 atcggcgagg tcgcggcggg ccgcccgttc gataccagcg ttggcgccgg cgaagcggtg
     4201 cggattttta ctggcggcgt gatcccgcaa ggcgccgacg ccgtcatcat ccaggaagat
     4261 accgtggccg aagcgggtgg catcaccatc accgaggcgg cgcgtccggg acggcatatc
     4321 cgccccgccg gcgtcgactt tcgcgagggc gatgtcctgc ttaaaggcgg aatacgcctc
     4381 accgaccggg atctctcgct ggctgcgggc atgaattatc ccgagcttgc ggtgcggcgc
     4441 cggcccaagg tcgctgtgct cgccaccggc gacgaattgg tgatgccggg ctctacccct
     4501 ggccccgggc agatcgtcta ttccaacggc tacgcgctgc gggcgctggc ccgtgccgag
     4561 ggcgccgaga ccatcgatct cggcatcgcc gccgacaccg tcgaggccac cacggaaggc
     4621 atccgccgcg cccgcgacag cggcgcggac atcctcatca ccacaggcgg cgcatcggtt
     4681 ggcgaccacg acctcgtcaa gcagtcgctg gaggccgaag gtgtcaccat ggcgttctgg
     4741 cggatcgcga tgcggccggg caagccgatg atgcacggac ggctcggcgc catgcgggtg
     4801 atcggcctgc ccggcaatcc cgtgtcatcc tacgtctgcg gattcctgtt tctggtgccg
     4861 ctgattcgct gcctttcggg tcggacgggc atccaccacg cccatgagac ggcgctgctc
     4921 ggacgcgatg tcgccgccaa tgacttgcgt gaagactatc tgcgcgcccg ccttgagatg
     4981 cgcagcgacg gcgtcttgat cgccacgccg gtcgaccatc aggacagctc gttgttggga
     5041 aatctcgctg cggcacgggc gcttgtgatc cgcgcgccgt ttgcggccgc ggctgccgcc
     5101 gaatctgttt gcgatatcct tcggcttccg ggctgacccc tcaagccggt cgctgtccgg
     5161 cgaaatcgtt tttgttgacg caaaattaag tggttgcaga acacatatgg aacatatagt
     5221 gttcgttcat gatttgtttc tgacgttgac gcctgcaaat tggagccacc gagtccgggc
     5281 ctttgactca gggcgtctcg gaatcgaacg gcactatcaa gcttagagac gcaaacttgg
     5341 ggattggtcg agatgctcac gcgcaaacaa tatgaactcc tgcggttcat cagcgaacgc
     5401 ctcaaggaat ccggcgttcc gccttccttc gacgagatga aggacgcgct ggatctgcgc
     5461 tcgaagtccg gcatccaccg cctcatcacc gcgttggaag agcgtggctt cattcgccgc
     5521 ctgcccaatc gcgcccgcgc catcgaagtc atcaagcttc ccgagctttc ggggagtagc
     5581 aataatggca atggccgccg cggcttcacg cccagtgtca ttgaaggcac gctcggcagg
     5641 gtccgcagca gcagcgttgg ctccgaagat gataacaacc gtccggttgc cgtgcctgtc
     5701 atgggccgca tcgccgccgg cacaccgatc gaggcgctgc agacccgcag tcacaccatc
     5761 tcggtgccgc cggacatgct cggttccgga gaacactacg ccctcgaggt gcgcggcgac
     5821 tccatggtgg atgccgggat tctcgacggc gacatggcgc tgatccagcg caatgaaacc
     5881 gccgagaccg gcgatatcgt ggtggcgctg atcgacgaag aggaagcgac gctaaaacgc
     5941 ttccgccgcc gcggcgcatc gattgcactc gagccggcca atacgtccta tgaagtccgc
     6001 atcctgccgc cgaaccgggt caagattcag ggcaagctga tcgggcttta tcggaagtac
     6061 tgagcgagcc ggccccaaaa gccctcggac gcgccgatcg acgcgtcgaa gaacggcgtc
     6121 tcgccaaata gtcgcggcca cccgcctctg ccgtcgtgtc ccgccaccgg gtctcgtcaa
     6181 cggcgccgat gacaggcgcc agcgggtggc atgcagtatt ccgcgacatt tccgggaatc
     6241 gggacagcgg ctggaccgcc ggctcgcgct gacggtgcgt ccacgcctcg catcggcccg
     6301 cattctcaaa cgcatttgtg atcaggcgcg tcgggtagag ccgcacaatt gcctacaacg
     6361 aggcaaaaac tcacggacta ctgtccactc tgatagaggc ctgcgcctcc gaggggcgcg
     6421 ggttgctatc tcgaacgagg agcaatccga tgtgtgacta tagtctgcac gccgtagcaa
     6481 cgcgccccgc ccgggttggg gagacgctca tcaccacgac attccgcggg acttcgaccc
     6541 gtggtttcgc ctcagaaagt gagcccgcgg tggcggtctg catgcttcct gggaccgaac
     6601 tggcgttcgc ccaaaatgtc aaatacgaca accgatggat ttggacgaaa accctgaatt
     6661 tccgggtggg caaattcggc gcgatcgagc ccgatgttgc gcatcgtcac catgatgcga
     6721 tcgaatttcc tgatggcagc catgtgctcg tgacgcagct ctgcgagggg cagcgagcga
     6781 ccgtgctgca attgccggtg actgatcggg tggcggggca cgcgccatcg gttgccaata
     6841 caaatgcagc aacgccggtt tccgttgact gaagttgatt aaagctgatt aaaatggtga
     6901 tttggatatc ccccggcggt tgcggccggc gggggtgtgc cgagtatcat ccatggatat
     6961 tgcccgcagc gaaccgcagg cgacacccgc cagggaccac cgggacgacg cgcgcgccga
     7021 agaactgact aatctgaggc tggcgctggc gacgttcgcg ttgcagctcg acgccttcga
     7081 aatgcgcacg agcagagtag cgcacggacc aggcgcgagg gccgtcaaga aggtcacatg
     7141 gcgacaggtc gggcgagtga ccgaaccggg ccgatacatg ttcaggttcg ggtggctcac
     7201 gattgcggcg gaagatcttg ctgtctggga gcagcttccg aacgcggcgt ttacgctggt
     7261 caggacggcg gccgaaatcg aggaatatcg tttgggcgca ttcgaactgc gggaagatct
     7321 cgcgcttcgt gagaagtgaa gcgctttttt caaagtattg gctcggcgtc ccgtcagtcc
     7381 tgacccctgc acgccggctt ccaacagcag cacatccggg ggatacccgc cgagagacgg
     7441 cttggcggcc gggtcgccaa ccggtaacgt ccaaactgcg ccgaaggcga gcgggataat
     7501 cgcggcttga aactgtggcg ctggtctata ccgttcgctt ggctcagtgc cgttttggag
     7561 aatcgatgat gaagaaggca gtcataatct tggcttcggt tgcagccctt gcggtgacct
     7621 cgctctcggt ccccagctcg gccgaggctc gcgcataccg gcatcggcac catagcggtt
     7681 atgcgctgta ttatgactat agtcatggca acacgctgtc agcgatgagc tacgtctacc
     7741 cggccgcgaa ttgggggcca tttttccacc gggtgcggca cttcggtccc gttcccgttg
     7801 tcccctacca gccggccgcc tggtagagac tgccggttcg ccggaattgc cgaacctggc
     7861 gcgggctctt tgccttcgcc tgcgcggctt gcgaataccc cgcgcttcgg tctctcgcca
     7921 ccggataaca gtgcctgcca aacccgccgg catgaaccgg cgtcttcatc gcatcgtcta
     7981 atggtcggcc gagttttgtc cggtaagtga cctggttcac atatgccccg ccccgactcg
     8041 gttaccaacg cttactggtc tcatcatgcc agggggcgag ctttggagat cgcaaatgac
     8101 tgctgtcgca gaggcccttt cgcgcgagtt tcccgcaacc acatcgcggc aaccaaatac
     8161 gaatatagac gttctcctgt cggccaatga gatggagcga ctgggcacta acgtgaaaga
     8221 gcatctcatt ttgggtaccg ataagcagga tgccgaacag caaagagacc tctggctgtc
     8281 agaaaatccg tctatcaggg tgatcaaaat tcacagagtg aagcgagagc cgcagacctt
     8341 gctgacactt atcggcagca aacacgtccc acgcgtttcg atcttggtgg agtacgagga
     8401 gaccgaggtc tccgcggcga acgtcgccgc cgaataaaag ccgctcattg acggcgactc
     8461 catcggaaga aagcgatgag cagagatatg ccgacagcgg cggctatcaa aactcccgtg
     8521 cgaatatctt cgcgggcgat gccgacttgg gtgtgaagga cgtgacgcaa cactgcggcg
     8581 gtcaccaccg ccgcgaggaa tatgccgagc aggtacctca tccgaagctt ggtcgggtcc
     8641 attacgcaga gtaacacccg cacaatttac ctttcaagac aaccctgagg cccaaggtca
     8701 ttttcccacc agcgaaatat gcgcgcgaac tgagttgcct atcagtcctc agcctgcagg
     8761 tcggcttccg atggcgtcgc atcgacagcg cgcggggccg tggggcgcgt catgaggttt
     8821 gtttcagcct cgatgtcgcc cgccatcgcc ggcgaccacg gccgatcgac gccttttggc
     8881 ttcaccgcat cgaccgcaaa tccggttgcg gttcgccgca gcgccatcgc gcccagcctt
     8941 cgcaaccgat cccgatcgat caccggcgac gcacatgaag gcggcgcctg cctcatggtc
     9001 accaccagcg ccgcgcgctc gcaatcgtcg gccagcgcgt caggccgcag cgccagcgtg
     9061 atcaacccgc cggcggcggt ctgcgccaca caacccgcct catcgcacga cacgccctcc
     9121 gcaagcgaag catccgtggg ctgccggggg tcggcgtcag cggccagcca ttccttcagc
     9181 aggaacacgt ccttggcggt ccgcatcaga tgca
//
LOCUS       DATNOI010000340         3970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_900610,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000340 DATNOI010000000
VERSION     DATNOI010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900610"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..356)
                     /locus_tag="VN968_13540"
     CDS             complement(3..356)
                     /locus_tag="VN968_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXL30297.1"
                     /translation="MLDRRQSTRDKIIYGGVAEIGGDGATRDCVVRNISEQGAKIEFS
                     TAVKLHKEQMSVAFARKGRSFLARVVWWRDNFVGVAFSSDSELPVSDLDERLRKSEQK
                     KRQLQRRIKELIGED"
     gene            complement(495..1430)
                     /locus_tag="VN968_13545"
     CDS             complement(495..1430)
                     /locus_tag="VN968_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HXL30298.1"
                     /translation="MTATPEGQLGFAPDDDAPIPYMARTREYYQAIGYTTPYRWAHYV
                     GAPFQSLRKPLAQSRVAIVTTAAPFDPARGDQGPGAKYNGGAKFYSVYDGDTSKPHDL
                     RISHIAYDRVHTSADDSGTWFPLPQMQRLTQEGRIGEVAPRLFGAPTNRSHRVTIETD
                     APEILARCLEDKVDAAVLVPNCPVCHQTISLVARHLEANGISTVVMGCAKDIVEHAAV
                     PRFLFSDFPLGNSAGKPHDARSQGFTLELALRLLETAPGSQTTVQSPLRWSADASWKR
                     DYNNVAMLSSDELARRRREFDAQKEIARGLRESAA"
     gene            1524..2555
                     /locus_tag="VN968_13550"
     CDS             1524..2555
                     /locus_tag="VN968_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXL30299.1"
                     /translation="MPTVIRRPAALGIMLLALVLPAARAIAEDAARVTAGEVARPSVE
                     ELALPKDDSQDENKAEVQKPADTARETDTREAMCLMIESAAKAQGLPLEFFARVIWQE
                     SRFQSDAVGPVTRNGQRALGIAQFMPGTANERRLLDPFDPVQALPKSAEFLNELRSQF
                     GNLGLAAAAYNAGPRRVQEWLAGSGPMPQETRNYVIAITGASVDDWAAASKNGNSNAR
                     MPDRAPSSNCRVLMALLRRAPNPFVTQLEQHVTLAAARLWGVQLAAGFSRDKALAMYA
                     RAMQRLSAVIGDQDPSLLSSVMRSRGSRLFYQVRIGADTRPAADDLCNRIRRAGGACF
                     VLRNMYLRG"
     gene            2621..2740
                     /locus_tag="VN968_13555"
                     /pseudo
     CDS             2621..2740
                     /locus_tag="VN968_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
     gene            2809..3807
                     /locus_tag="VN968_13560"
     CDS             2809..3807
                     /locus_tag="VN968_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL30300.1"
                     /translation="MMGKRMNLLARPALICCLLIAAFAATPNASAVDYPVRPIKLVVP
                     YAAGGPTDVLGRLVADYLGRDLKQAVIVENKPGAQGAIGAEAVARADPDGYTLFVAAG
                     SIIVLNPMLYKKLSYDPVKDFRMLALVTDLPVVMEVHPSVPAKTVAEFVAYAKQNPGK
                     LNFGSAGTGGTIHLAGEMFKQMAGIEMTHVPYKGAGPALTDLLSGNIQVMFDSMGTAL
                     PPVKAGLLRPLGVSSTQRSPDLPDVPTIAESGYPDYAVSVWYGIVAPSKLPDEIAQKI
                     SASLDRALNDDTFRASLEKIGFPVFRPRSAAAITEFIDADRARWSAVIRAQNISLD"
     gene            3812..>3970
                     /locus_tag="VN968_13565"
     CDS             3812..>3970
                     /locus_tag="VN968_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HXL30301.1"
                     /translation="MVRESEIREGELAVEMPKTTNAGLVFIGRIRTPWTSRLETPRQG
                     RHDGPVCRL"
BASE COUNT          723 a         1226 c         1258 g          763 t
ORIGIN      
        1 gttcagtctt cgccgataag ctccttgatg cggcgctgaa gctgccgttt tttctgctcg
       61 ctcttgcgca gccgctcatc aaggtcggag accggtagct cggagtccga actgaacgca
      121 acgccgacga agttgtcgcg ccaccagacc acccgggcga ggaaagagcg gcctttgcgt
      181 gcgaaggcca ccgacatttg ctctttatgg agcttaacag cggtgctgaa ttcgatcttg
      241 gcgccttgtt cggagatgtt gcggacgacg caatccctgg tcgcgccatc gccgccgatt
      301 tcggcgaccc cgccatagat aatcttgtcg cgcgtgctct gacgtcgatc caacatcgaa
      361 tgcactcccc aaaaaggagg ggaccttacg cggcttcgcc gcatgcgaaa ggccgcaacc
      421 ggcaaaaagc ggtgttaacg taaaccgatc tcgaggaaaa tcgaccgaat tccggggcag
      481 ggcgcggctt aaccttacgc agcgctctcg cgcaatccgc gcgcgatttc cttctgcgcg
      541 tcgaactcgc gccgccgtcg cgccagttcg tcggagctaa gcattgctac attgttgtag
      601 tcacgcttcc aggacgcatc tgcgctccag cgcagcggcg attgaacggt ggtctgcgaa
      661 cccggcgctg tttccagcag gcgcagcgcc agctccagtg tgaagccttg cgagcgggcg
      721 tcgtgaggct tgccggccga gttgcccagc ggaaaatcac tgaacagaaa ccgcggcacc
      781 gcggcgtgct cgacgatgtc cttggcgcac cccatcacga ccgtggaaat cccgttggcc
      841 tccagatgcc gggcgaccag gctgatggtc tggtggcaca ccgggcagtt aggcaccagc
      901 accgcggcat ccaccttatc ttccaggcag cgcgcgagga tttcgggtgc gtcggtctcg
      961 atggtgacgc gatggctgcg gttggtcggt gcgccgaaca agcgcggcgc aacttcgccg
     1021 atccgtccct cttgcgtgag ccgctgcatt tgcggcagcg ggaaccaggt tccactgtcg
     1081 tcggccgagg tgtgcacgcg atcataggcg atatgggaaa tccgcaagtc atgcggcttc
     1141 gaggtatcgc cgtcatagac agaatagaac ttggcgccgc cattgtattt ggcgcccggg
     1201 ccttggtcgc ccctggcggg atcgaacggc gcggctgtcg tgacgatggc aacgcgggac
     1261 tgggcgaggg gcttgcgcaa ggactggaat ggcgcaccga cataatgcgc ccagcgatag
     1321 ggcgtggtgt agccgatcgc ttgataatat tcgcgggtcc gcgccatgta agggatcggc
     1381 gcatcgtcgt cgggcgcgaa accgagctgg ccttcaggcg ttgctgtcat gtcttgcgct
     1441 ccggctcgcg cgctatcatt tcttgaattg ctaaccggtc ggttccccgt gtcaagcacg
     1501 gtctcacgta aaacagaacg gcaatgccaa cggtcattcg tcgccctgct gccctcggca
     1561 tcatgttgct ggcgctcgtg ttgccggcag cgcgggccat cgccgaagac gctgcgcgtg
     1621 tcacggccgg cgaggtagca cgaccaagcg tcgaagaact cgcccttccc aaggatgaca
     1681 gccaggatga aaacaaggct gaggtgcaaa agcctgccga caccgcccgg gagaccgaca
     1741 cccgtgaggc gatgtgcctg atgatcgaat cggcagcgaa ggcgcaaggc ttgccgctcg
     1801 aatttttcgc gcgtgtgatc tggcaggaaa gccgctttca atccgatgcc gtgggacccg
     1861 tcacccgcaa cggccagcgc gcactcggca tcgcgcagtt catgcctggg accgccaacg
     1921 agcgccgcct gcttgatccc tttgatccgg tgcaggcgct gccgaaatcg gcggagttct
     1981 tgaatgaatt gcgcagtcaa ttcggcaatc tcggccttgc cgctgcggcc tacaatgccg
     2041 gaccgcggcg ggtgcaggaa tggctcgccg ggtcaggccc gatgccgcag gagacccgca
     2101 actacgtcat cgccattacc ggcgcatcgg tcgatgactg ggccgccgcc agcaagaatg
     2161 gcaacagcaa tgccaggatg cccgaccgcg cgccaagctc aaattgccgc gtgttgatgg
     2221 cgcttctgag acgcgcgccc aaccccttcg tcacccagct tgagcagcat gtgacgctcg
     2281 ccgccgccag gctgtggggc gtgcagttgg ccgccggctt cagccgcgac aaggcactcg
     2341 cgatgtatgc gcgcgcgatg cagcgcttga gcgctgtcat cggcgaccag gacccgagcc
     2401 tcttgagctc ggtgatgcgg agccgtggca gtcgcttgtt ctatcaggtg cgtatcggcg
     2461 ccgacacgcg tcccgctgcc gacgatctct gcaaccgcat ccggcgtgcg ggcggcgcct
     2521 gtttcgtgct gcgcaacatg tacctcaggg gctgagcgga cggttcaccc gttgatcatt
     2581 gcggtgatcg acttcatcga gcagcaaccg ctgaaaacct atacgctatg gcgcggtggc
     2641 gggcggattc gcaggcctca tcgcgacccg gcgttcgatt tttgccggcg tcgtctgcgg
     2701 tgaggtcatc atgctgatcg gcaagtggtg gctcggctga ggcgcactcg agcacgaagg
     2761 tcggctttgc ggccaagcca tattccgtta gggtccggca actcaaaaat gatggggaaa
     2821 cgtatgaatc tgctcgccag gccggccttg atctgctgcc ttctcatcgc ggcatttgcc
     2881 gccacgccga atgcgagcgc ggtggattat cccgtacgtc cgatcaaact cgttgtcccc
     2941 tatgcagccg gcggtccgac cgacgtgctt ggccggctgg tggccgatta tctcggccgg
     3001 gacctcaagc aggctgtcat cgtcgagaac aagccgggcg cgcagggcgc gatcggggcc
     3061 gaggcggtgg cgcgcgccga tcccgacggc tacacgctgt tcgtggcggc gggttcgatc
     3121 atcgtgctca atccgatgct gtacaagaaa ttgtcctatg atccggtgaa ggattttcgg
     3181 atgctggcgc tggtcaccga tctgcctgtt gtcatggagg tccatccttc ggtgcccgcc
     3241 aagacggtgg cggagtttgt cgcctacgcc aaacaaaatc ccggcaagct caatttcgga
     3301 tcggccggca ccggcggcac cattcacctc gcgggcgaaa tgttcaaaca gatggccggc
     3361 atcgagatga cccatgtacc ctataagggc gccggtccgg cattgactga cctgttgtcc
     3421 ggcaacatcc aggtgatgtt cgattcgatg ggcacggcgt tgccgccggt aaaagccggc
     3481 ttgttgcggc cgctgggcgt gagttcgacg cagcgaagcc ccgatctgcc tgacgttccg
     3541 accatcgccg agagcggcta tcctgattat gccgtcagtg tctggtatgg cattgtcgcg
     3601 ccgtcaaagc tgccggacga gatcgcgcag aagatcagtg cgagcctcga tcgagcctta
     3661 aacgacgata cgttccgggc ctcgctggag aagatcggct ttcccgtgtt ccgcccgcgc
     3721 agtgctgccg cgatcaccga attcatcgat gccgatcgtg cacgctggtc cgccgtgata
     3781 agagcacaga acatttcatt ggattgagaa tatggttcga gagagtgaaa tccgcgaagg
     3841 cgaactcgcg gtcgagatgc ccaagacgac caatgccggg ctggtcttca tcggccgtat
     3901 ccgcacgccc tggacttcgc gtctcgagac gccgcggcag gggcgtcacg acggcccggt
     3961 ctgccggctc
//
LOCUS       DATNOI010000341         7582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_210372,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000341 DATNOI010000000
VERSION     DATNOI010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210372"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VN968_13570"
     CDS             complement(<1..389)
                     /locus_tag="VN968_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30302.1"
                     /translation="MCRPVLILAPVAMLGFGALALWLGQDASFDQLNYHFYGPWALLH
                     RRLLVDVFPAFTGPTFHNPSADVPFYLLAAHAPPRVTGFVLGAVHGLAAVPLYLIARA
                     LVPENRLVALLIVLAGMAGAMSVAETGT"
     gene            569..1591
                     /locus_tag="VN968_13575"
     CDS             569..1591
                     /locus_tag="VN968_13575"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HXL30303.1"
                     /translation="MQIDRRPLFDRLAVFPRLGLANLPTPLEPMKRLTAHLGGPRLWV
                     KREDATGLGFGGNKLRKLDYVLHAALSSGADTIVSGGVVQSNSQRQVAAVAAKLGLAC
                     HLAVYHGRLEPPTPEYKTSGNAFLNRLFGAHLHDVPWTGDRNAAIRTLVGDLEARGHR
                     PYFVPYGVSNALGAVGYATTISEIEPQAALLGFAPTAVVHCTGSAGTQAGLVVGAAVA
                     MPNTRIVGIDIDAEPTRVRADVVALAREASDLLDVPFNEATVEVVAGHAGPAYGVPHE
                     ATIEAIRLAGRLEALPLDPVYSGKGLAGLIALIRQGRWRKDEDVVFLHTGGVPALFAY
                     QSALGI"
     gene            1971..3065
                     /locus_tag="VN968_13580"
     CDS             1971..3065
                     /locus_tag="VN968_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015886668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXL30304.1"
                     /translation="MEVTSERNSAERPEATGGAGHEPAFSNQIRTVNLALQGGGAHGA
                     FTWGVLDRLLDEKDLAFEGLSATSAGAMNAAVFAYGLAVDGREGARKALTGYWKRVSD
                     AARLGPLQPNPIDRMLGDHKLTWSPIFSVIDFVTRVLSPYQFNPANYNPLRDLVEQSI
                     DFEVLKRPDCPVKLFLSATNVRTGKVKIFSGTEISVSAVMASACLPTMFHAVEIDGEA
                     YWDGGYMGNPALFPLIYNCKSTDIVTVHINPLFRKEVPRAAGDILNRINEISFNSSLM
                     REMRAVSFVTKLITQNRVVDGGLKRVLIHSIADDEFMGALSPTSKYNADWDFLIFLRD
                     QGRKCAGNWLAKNFVKLGVESSVDIDKMYL"
     gene            complement(3127..4071)
                     /locus_tag="VN968_13585"
     CDS             complement(3127..4071)
                     /locus_tag="VN968_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HXL30305.1"
                     /translation="MSRPHRFGFLLIDDFALMSFASAVEPLRAANILAGRTLYEWFHI
                     STGAGAIAASNGLSVTADHTIADKIRFDTVLVCAGGNPASFEDAKAIAWLRRLARSGC
                     EIGGVSGGPFILAKAGILDGVRCTIHWEHLPAFTEAFPDLVSTRTLFEIDRGRLTCGG
                     GVAALDMMHALIRRDHGQSLAARVSDWYLQTAVRLGDSTQRISLLERVGTNNRSLIAA
                     IGSMERQLASPMSRPALAKAAHVSVRQLERLFAKHLGVTIGQHYLALRLTKAQALLRQ
                     TSLPIVQIGAESGFANASHFARVYRQRFGTLPSKDRMR"
     gene            4191..5441
                     /locus_tag="VN968_13590"
     CDS             4191..5441
                     /locus_tag="VN968_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HXL30306.1"
                     /translation="MRYSLTSLLVNALAGHKGWKPQWRDAEPRDEYDVVIVGGGGHGL
                     STAYYLAREYGITDVAVLEKGWIGGGNVGRNTTIVRSNYLLPGNIPLYELSMKLWEGL
                     ERDFNYNAMVSQRGVLNLYHSDGQRDAYARRGNAMRIHGVDAELLDQDGVRRLYPFLN
                     FDSARFPIQGGLLQRRGGTVRHDAVAWGYARGASDCGVDIVQNCEVTGFAISDGRVTG
                     VQTTRGSIRANKVAIAVAGSSSRVAAMAGMRLPIESHVIQAFVSEGLKPIIPGVITFG
                     AGHFYISQSDKGGLVFGGDIDGYNSYAQRGNLPVVEDVCEGGMALMPAIGRARILRNW
                     GGLLDMSMDGSPIIDRTPTQGLYLNAGWCYGGFKATPGSGFVFAHLIARDAPHPAALH
                     LRLDRFRTGHLIDEKGQGAQPNLH"
     gene            5448..5723
                     /locus_tag="VN968_13595"
     CDS             5448..5723
                     /locus_tag="VN968_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HXL30307.1"
                     /translation="MRIPCPFCGPRYTAEFSYLGDAKPKRPDTAMASADIDAALYDYV
                     YLRDNPAGPLREYWYHGSGCHSWLVVERDTRTHRIIAASMAQAEPLP"
     gene            5720..>7582
                     /locus_tag="VN968_13600"
     CDS             5720..>7582
                     /locus_tag="VN968_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXL30308.1"
                     /translation="MTGPRRLANGGLIDRTKTIRFSFDGNPLQGLGGDTLASALMANG
                     VRLVGRSFKYHRPRGIVSAGPEEPNALVELRSGARREPNTKATTVELFDGLEATSQNR
                     WPSLAHDIHAINQLAGPLLVAGFYYKTFMWPAAFWEKIYEPAIRRAAGLGRLSGEADP
                     DTYEKAYAFCDLLVIGAGPAGLAAALTAGRAGARVILAEDDFVTGGRLNSETHEIDGA
                     PGTIWARRVEEELAALPNVRVMRRMSVFGAYDGEYGAIERVADHVPAPPAFTPRQRLW
                     KIVARETILATGAVERGIVFGGNDRPGVMLASAMRTYVNRFAAAPGRHIAVFTASDDG
                     WRTAFDLGRAGVAVACVVDIRTKVAPEVLALAGDIKIFLGGRVTDARGGRALKSIAVI
                     DATGNRSAMAVDALAVSGGFNPDIALSTHLGSKPAWSEPKAAFLASELPDGMAIAGAA
                     AGHWTLAEALHDGTVLGADAAARAGRRPQGMLRYRTSDEQYAVNAFWHVAESRRKAFV
                     DLQNDVTDKDVALAAREGFRSVEHLKRYTTLGMATDQGKTSNVSGLAIMAELTGRTIV
                     QTGVTRARPPHVPVAIGAFAGLHTGRHFKPTRLTSGHGWAAAQGATFVDAGPWLR"
BASE COUNT         1388 a         2381 c         2466 g         1347 t
ORIGIN      
        1 gtgccggtct cggcgaccga catcgcgccc gccatgccgg cgagcacgat cagcagggcg
       61 acgagccggt tctccggcac cagggcgcgg gcgatgagat agagcggcac cgcggcgagg
      121 ccgtggaccg cgccgaggac gaagccagtg acacgcggcg gcgcatgcgc ggcgagcaga
      181 tagaacggaa cgtcggcaga cggattgtgg aacgtcggtc cggtgaaggc cggaaacacg
      241 tcgacaagga gccgccggtg cagcagtgcc cacggcccgt agaagtgata gttcaactgg
      301 tcgaagcttg cgtcctgccc gagccacagc gccagcgcgc cgaagcccag catcgcgacc
      361 ggtgccagga tcaggacggg acggcacatg acggtgtgag cgaataaaaa gcgacgtagc
      421 tgctgggcag cagcgaaaat ggcgcggtgg ggccacgagg agacttccat cgttcgctcg
      481 aatgtgatgc agctatcgtc gatggggcta tcatagcggc cgtcgggcta ttgatggaat
      541 atcacttgtg agtcagacgg acagaacgat gcaaattgat cgcaggccgt tattcgaccg
      601 actcgctgtc tttccgcgct tgggcttggc caatctgccg acccccctcg agccgatgaa
      661 gcgactgacg gctcatctcg gcggaccgcg gctgtgggtc aagcgcgaag atgccaccgg
      721 gctcggtttt ggcggcaaca agttgcgtaa gctcgactac gtcctgcacg cggcactttc
      781 gagcggcgcc gatacgatcg tctcaggtgg cgtcgtgcaa tcgaacagcc agcggcaggt
      841 tgcggcggtt gcggcaaagc tcggcctcgc gtgccatctt gcagtctacc acggccgact
      901 cgagccgccg acgccggaat acaagacctc gggcaacgcg tttctcaatc ggctgttcgg
      961 cgcgcacctg catgacgtgc cgtggaccgg tgaccgcaat gcggccattc gcacacttgt
     1021 cggcgacctc gaagccaggg gtcacaggcc gtactttgtg ccatacgggg tctcgaatgc
     1081 cctgggcgca gtcggttatg ccacgacgat ttccgagatc gaaccgcagg cagcgctatt
     1141 gggcttcgca ccaaccgctg tcgtgcactg caccggaagc gccggcacgc aggctggtct
     1201 cgtcgtcggt gcagccgtgg caatgccgaa cacgcggatc gtcgggattg atatcgacgc
     1261 cgagccgacg cgggtgcgag ccgatgtcgt ggcgcttgcg cgagaagcgt ccgacctgct
     1321 tgatgttccg ttcaacgaag caacggtgga agtcgtcgcg gggcatgcgg gacctgccta
     1381 cggggtcccg cacgaagcca ccatcgaagc gatccggctt gcaggccggc tcgaggctct
     1441 tccacttgat ccggtctatt cgggcaaggg gctcgcgggc ttgatcgccc tgatccgcca
     1501 agggcgctgg cgcaaggacg aggatgttgt cttcttgcac acaggcggcg tgccagcgct
     1561 tttcgcctac cagagcgcac tcggcatctg aactggacat cccccttgtc cagcggaccg
     1621 cgcgtgaagt agcgatccgc gattgcgatc agggtgaatg tccgcggtga gtcgaactca
     1681 gacttcggta ttgagagtct gaacaagccc ttgtgatctc gggaaccgcc gcccgcagtg
     1741 gggcaattac tttcaagcgg ggtcgacgtt ggtcacgccg cacgcggcgc aattgttgcc
     1801 caaccgggat aacctgtcgc cttctgactt ggaaaaaaag ggcgaggctt gcgtcggctg
     1861 ccgtccattt gtgttatgca tgtccatgtg atcgacgtac gtttctgcac ctccgaataa
     1921 tccaattgtt gccattgaat gctttgcgca tgcaggcgag tagggttgct atggaagtta
     1981 cttcggagcg gaactcggcg gaaaggcctg aggcgactgg gggagccggt cacgaaccgg
     2041 cattctcaaa ccagatcaga accgtcaacc tggccctcca gggaggcgga gcgcatggtg
     2101 ccttcacctg gggggtgctc gaccggctcc tggacgaaaa agacctggcc ttcgaggggc
     2161 tcagcgcgac cagcgccgga gcgatgaatg cggcagtctt tgcctatggg cttgcggtcg
     2221 acggtcgcga aggggcacgc aaagcgctca caggctactg gaagcgtgtc agcgatgcag
     2281 ccaggctggg gccgctgcag cccaacccca tcgaccgaat gctgggcgat cataaattga
     2341 cgtggtcgcc gattttttcc gttattgact tcgtgacgcg cgtgctgtcg ccataccagt
     2401 tcaaccccgc gaactataac ccattgcgcg acctggtcga gcaatcgatc gattttgagg
     2461 tgttgaaacg gccggactgt ccggtcaagc tcttcctgtc tgcgacgaac gttcgtactg
     2521 gcaaggtgaa gattttctcg gggacggaaa tctcggtatc cgccgtgatg gcttcggcgt
     2581 gcctgcccac gatgtttcac gctgtggaga tcgacggcga agcatattgg gacggcggat
     2641 atatgggaaa ccccgcgctc ttcccgctaa tctacaactg caagagcact gatatcgtca
     2701 ccgttcatat caatccgctg ttccgcaaag aggtgccgcg cgccgcgggc gatattttga
     2761 atcgcatcaa cgagatcagc ttcaattcgt ccttgatgcg ggagatgcgg gcggtcagct
     2821 tcgtgaccaa gctcatcacg caaaaccgcg tcgtcgatgg aggactgaag cgtgtgctga
     2881 tccacagcat cgccgacgac gaatttatgg gtgcgctcag tcccacaagc aagtacaatg
     2941 ccgattggga ctttctgatt tttctccgcg accaggggcg aaaatgtgcg ggcaactggc
     3001 tcgcgaagaa cttcgtcaaa ctgggtgtcg aatcatcggt agacattgat aaaatgtacc
     3061 tctgaacctg ggggtcgtgc cgagaaatgc taggggtccg ttgtctcgcg gcatatcggc
     3121 gccgcttcat cgcatccggt cctttgacgg cagcgttccg aacctctgcc ggtagacccg
     3181 cgcaaaatgg ctggcattgg caaacccgga ttcggcgccg atctggacaa tcggcaggct
     3241 ggtctgccgc agcagcgcct gggcctttgt caggcgcagc gccaggtaat gctggccgat
     3301 cgtgaccccg aggtgttttg cgaacagccg ttcaagttgg cgcaccgaca cgtgcgcggc
     3361 tttcgccaat gccggccgtg acatcgggct ggcgagctgt cgctccatgc tgccgatcgc
     3421 ggcaatcagg ctccggttgt tggttccgac acgctccagc aacgagatgc gctgcgtgct
     3481 gtcgccgagc cggaccgccg tctgcaaata ccagtcgctc acccgcgccg cgagcgactg
     3541 gccgtgatcc cgccggatca gcgcgtgcat catgtccagc gcggcgacgc cgccgccgca
     3601 ggtcagacgt ccgcgatcga tctcgaacag cgttcgtgtg gagacgagat ccggaaacgc
     3661 ctcggtgaag gcaggcagat gctcccagtg gatggtgcaa cggacgccat cgagaatgcc
     3721 tgccttggcg aggatgaatg ggccgcccga gacgccaccg atctcgcagc cggaccgcgc
     3781 cagccttcgc aaccatgcaa tcgccttggc atcctcgaac gaggccggat ttccgccggc
     3841 gcagaccagc acggtgtcga accgtatctt gtcggcaatg gtgtgatcgg cggtcaccga
     3901 gaggccattc gaggccgcga tggcgccggc gccggtcgag atgtgaaacc attcgtagag
     3961 cgtccggccg gccaaaatat tcgccgcccg caacggctcg accgcggatg cgaacgacat
     4021 cagcgcaaaa tcgtcgatca gcagaaagcc gaagcggtgc ggacgggaca tggcgatgtc
     4081 gtcgttggtg ggatgtcgca attagaacat ttgatgtcgc taacagcgta tagccggttt
     4141 cgtttccgcg cgaaagtcgg ccgttccgat ccaaccagcc cggtcgcgcc atgcgctatt
     4201 cgctgacatc cctgctggtc aacgcgctcg ccggccacaa gggctggaag ccgcaatggc
     4261 gcgacgccga accccgcgac gaatatgacg tcgtgattgt cggcggcggc ggtcatggcc
     4321 tttcgaccgc ctattatctg gcgagggagt acggcatcac tgacgtcgcg gtgctcgaaa
     4381 agggatggat cggcggcggc aatgtcggcc gcaacaccac gatcgtccgc tccaactatt
     4441 tgctgcccgg caacatcccg ctttacgaac tctcgatgaa gctgtgggaa ggactggagc
     4501 gggatttcaa ttacaacgcg atggtcagcc agcgcggcgt gttgaacctg tatcactccg
     4561 acggtcagcg cgacgcctat gcgcggcgcg gcaacgcgat gcggattcac ggggtcgacg
     4621 ccgaactgct cgatcaggat ggcgtgcgaa ggctgtatcc ctttctcaat ttcgacagcg
     4681 cccggtttcc gatccagggc ggcctgctgc agcgtcgcgg cggcaccgtc cggcacgacg
     4741 cggtggcatg gggttacgcc cgcggcgcga gcgactgcgg cgtcgatatc gtgcagaact
     4801 gcgaagtcac cggttttgcc atatccgatg gccgcgtcac cggcgtgcag acaacgcgcg
     4861 gatcgatccg cgccaacaag gttgcgatcg cggtcgccgg cagcagttcg cgcgtcgccg
     4921 ccatggccgg aatgcggttg ccgatcgaga gccacgtcat ccaggccttt gtctcggaag
     4981 ggctgaagcc gatcattccg ggcgtcatca ccttcggcgc ggggcatttc tacatcagcc
     5041 agtcggacaa gggcgggctc gtgttcggcg gcgacatcga cggctacaac tcctatgccc
     5101 agcgcggcaa ccttcccgtg gtggaagatg tctgcgaggg cggcatggcg ttgatgccgg
     5161 cgatcggacg ggcgcgcatc ctgagaaact ggggcggcct cctggacatg tcgatggacg
     5221 gctcgccgat catcgaccgc acgccgacgc aaggcctcta tctcaacgcc ggctggtgct
     5281 atggcggctt caaggccacg cccggatccg gatttgtctt cgcccatctg atcgcgcgcg
     5341 acgcgccgca cccggcggca cttcatctcc gcctcgacag attccgcacc gggcacctca
     5401 tcgacgaaaa gggccagggc gcccaaccga acctgcattg aggcgccatg cgtatcccct
     5461 gcccattttg tggaccccgc tacaccgccg aattcagcta tctcggcgat gccaagccaa
     5521 agcggcccga caccgccatg gccagtgccg acatcgatgc agcgctgtac gactatgtct
     5581 atctgcgtga caacccggcg gggccgctca gggaatattg gtatcacggg tccggctgtc
     5641 acagctggct cgtcgtcgag cgcgataccc gcacgcatcg gatcatcgca gcgtcgatgg
     5701 cgcaggcgga gccgttgcca tgaccggccc ccgacgtctg gcgaatggtg gtctgatcga
     5761 ccggaccaaa accatccgct tcagctttga cggcaacccg ctgcaaggtc ttggcggcga
     5821 cacgctggca tcggcgctga tggccaatgg cgtgcggctg gtcggccgct ccttcaagta
     5881 tcaccggccg cgcgggattg tctccgctgg ccccgaggag ccgaacgcgc tggtcgaact
     5941 gcgctcgggc gcgcgacgcg agccgaatac caaggcgacc acggtcgaat tgttcgacgg
     6001 gctggaggcc accagccaga accgctggcc gtcgctcgcc cacgatatcc acgccatcaa
     6061 ccagcttgcg ggcccgctgc tggtcgccgg cttttactac aagaccttca tgtggccggc
     6121 ggcgttctgg gagaaaatct atgagcccgc gatccgccgc gccgcgggcc tcggccgcct
     6181 cagcggcgag gccgatcccg acacctacga aaaagcctat gcgttctgcg acctgcttgt
     6241 catcggcgcg ggtccggcgg gacttgccgc ggccttgacc gcggggcgcg ccggggcgcg
     6301 cgtcattttg gccgaagacg actttgtcac cggcggacgg ctcaattcgg agacgcacga
     6361 gatcgacggc gcgccaggca cgatctgggc acggcgcgtg gaggaagagc tggcggcatt
     6421 gccgaatgtg cgcgtgatgc ggcgcatgtc ggtgttcggc gcctatgacg gcgaatacgg
     6481 cgcgatcgaa cgggtcgccg atcatgttcc ggctccgccg gccttcacgc cgcggcagcg
     6541 gctgtggaag atcgtcgcac gcgaaaccat ccttgccacg ggcgccgtgg agcgcggaat
     6601 cgtgttcggc ggcaacgata ggcccggcgt catgctggcg tcggcgatgc gcacctatgt
     6661 caaccgcttc gcggcagccc cgggccggca tatcgcggtc ttcacagcca gcgacgatgg
     6721 ctggcgcacc gctttcgacc ttgggagagc cggcgtggcg gtggcatgcg tcgtcgatat
     6781 caggacgaaa gtcgcaccgg aggtgctcgc gcttgccggc gatatcaaga tttttctcgg
     6841 tggccgggtt accgacgccc gtggcggccg cgcgctgaaa tcgatcgccg tcatcgacgc
     6901 gacaggcaat cgaagcgcga tggcggtcga tgccctcgcc gtttccggcg gctttaatcc
     6961 ggatatcgcg ctatcgaccc atctcggctc aaaacccgca tggtcggagc cgaaggccgc
     7021 gttcctggcc agcgaattgc ctgacggcat ggccatcgcg ggtgccgcgg ccggccactg
     7081 gactctcgcc gaggccctgc acgacggaac ggtgttgggg gccgacgccg cggcgcgcgc
     7141 cggccgccgg ccgcagggca tgttgcgtta ccgcaccagc gacgagcagt acgcggtgaa
     7201 cgccttctgg cacgtcgcgg agtcgcgcag gaaggccttt gtcgatctgc agaacgacgt
     7261 caccgacaag gacgtcgcgc tcgcggcgcg ggaagggttt cgctcggtcg aacatctcaa
     7321 gcgctacacc acactcggca tggcgaccga ccagggcaag acctccaacg tcagcgggct
     7381 cgccatcatg gctgagctca ccgggcgcac catcgtccag accggcgtga cgcgggcgcg
     7441 gccgccgcac gtcccggtcg ccatcggcgc cttcgcgggc ttgcataccg gccggcattt
     7501 caagccgacc cgcctcactt cagggcatgg ctgggccgcc gcccagggtg ccaccttcgt
     7561 cgacgccgga ccgtggctgc gc
//
LOCUS       DATNOI010000342         3787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2522019,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000342 DATNOI010000000
VERSION     DATNOI010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2522019"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="VN968_13605"
     CDS             <1..122
                     /locus_tag="VN968_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL30309.1"
                     /translation="GMGLSIARTIVEAHHGRIWAENKAGGGAVFHISLPLSAT"
     gene            324..1610
                     /locus_tag="VN968_13610"
     CDS             324..1610
                     /locus_tag="VN968_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HXL30310.1"
                     /translation="MSTRHGTGATLALPRIFQHEATVIEPVLLLGIPVDFILFALTLL
                     GVALFHRHTLPIALTGLAAIVVYKLAFSGFKFGAGLGGLGQHMAHEWVMLANLFLLLM
                     GFALLSRHFEESRIPDEMPALLPDDWTGGVVLLFLVFVLSSFLDNIAAALIGGTVARH
                     VFRGKVHIGYLAGIVAASNAGGSGSVVGDTTTTMMWIAGVSPLSVLEAYVAAIVAMLV
                     FAVPVSLQQQRFSPILKNPPTGLKIDPVRVFIVAAILVAALAANLTANLKFPALLDTI
                     PVIGLAVWAIILLTAGLRAPDWSVMPETFKGTIFLLALVTAASMMPVEKLPAAAWQTA
                     LGLGFVSAVFDNIPLTALALKQGGYDWGYLAYAVGFGGSMIWFGSSAGVALANMYPEA
                     KSAGRWVSQGWPVVAAYVIGFFVMLAVIGWHPDAPH"
     gene            complement(1623..2561)
                     /locus_tag="VN968_13615"
     CDS             complement(1623..2561)
                     /locus_tag="VN968_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HXL30311.1"
                     /translation="MIGVLVFPQFQLLDAAGPVSVFEIAARFAGQSPSIKVLAVTPGP
                     VRSSSGVEMLARGLKPYGAITTLLVAGGEGVEAAARCDKTLDFVRTMARRGIRLASVC
                     SGAYILAEAGLLDGRRATTHWRRTRHFLITYPEVKLEPDRIFVRDGNIWSSAGITAGI
                     DLALAMVTEDFGDEIAQKTARQLVLYNRRSGGQSQFSSLLELKAPNGRFGPLLSWVRE
                     HLDAPLTVENLAEQAGMSSRHFTRAFIAETGTTPSKAVERLRIEVARERVQSSGEAIE
                     RVAQLTGFRDPERMRRAFIRAFGQPPQSLRRAARAG"
     gene            2657..3343
                     /locus_tag="VN968_13620"
     CDS             2657..3343
                     /locus_tag="VN968_13620"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HXL30312.1"
                     /translation="MPEPLQIGLLIFPKVTQLDLNGPVQVFSSVPGAKVHLIWKRIEP
                     VTSDSVLTLTPTITFADCPQLDVICVPGGFGTDEMVNDEEVLGFLRQQAKGAKYITSV
                     CTGSLVLGAAGLLQGYRATTHWLAMDNLAPFGATPAKTRVCVDRNRVTGGGVTAGIDF
                     ALTLVSIMVDRQTAEAIQLRLEYNPAPPFNAGSPDTAPPEVLALMKERYAASQARRGE
                     AVRRAAARLA"
BASE COUNT          644 a         1198 c         1215 g          730 t
ORIGIN      
        1 tgggaatggg attgtccatc gcccgaacga tcgtcgaagc ccaccacggt cgaatatggg
       61 cagagaacaa ggccgggggc ggcgcggtgt ttcacatcag tctgccgcta tcggcaacgt
      121 aacgccttcg agacaggctc caagggcaca aatgcgaagt gccataacgt ccgctttgct
      181 gccgctctcg cggggtaagc ggaagtgacg cagacatcgg caaaccggcg tgaatgaatc
      241 aggctgtgcg ctgcggtgcg gaattttgtc gctgccgggg catggcggac cttggtcaag
      301 cccgcaccag tcaagctcga tttatgagta cgcgccacgg tactggcgct acactggcgc
      361 tgcccaggat tttccaacat gaggccactg tgatcgaacc cgtacttttg ctcggaatac
      421 ccgttgattt cattctcttc gcgctgacgc tgctcggcgt tgcgctcttc catcgtcaca
      481 cgcttccgat cgcgctgacg gggctcgccg ccatcgttgt ttacaagctg gcgttcagcg
      541 gattcaaatt cggcgcagga ctcggcggcc ttggccagca tatggcgcac gaatgggtga
      601 tgctcgccaa tctgtttttg ctgctgatgg gatttgcgct tttgtcccgg cacttcgagg
      661 agagccgaat tccggacgag atgccggcct tgcttcctga cgactggacc ggcggcgtcg
      721 tgttgctgtt cctggtgttc gtgctgtcga gcttcctcga caacatcgcg gcggcgctga
      781 tcggcggaac ggtcgcgcgg cacgtttttc gaggcaaggt ccatatcggc tacctcgccg
      841 gaatcgtcgc ggcatcgaac gccggcgggt cgggcagcgt ggtcggcgac accacgacga
      901 ccatgatgtg gatcgcgggc gtcagtccgc tcagcgtgct cgaagcctac gtcgccgcga
      961 ttgttgcgat gctcgtgttc gcggtgcccg tatcgctgca acagcagcga ttttctccga
     1021 tcctcaagaa cccgccgacg ggactgaaga tcgacccggt gcgggttttc atcgtggcag
     1081 ccattctcgt tgccgcgctg gccgccaacc tgacggcaaa cctgaagttt cctgcccttc
     1141 tggacacgat ccccgtcatc ggcctcgcag tctgggcgat tatcctgctt accgcaggat
     1201 tgagagctcc cgactggtcg gtgatgccgg agactttcaa gggcaccatc tttttgctcg
     1261 cactggtaac cgcggcttcg atgatgccgg tcgaaaaact gccggccgct gcatggcaga
     1321 ccgccctcgg cctgggcttt gtctctgccg ttttcgacaa tatcccgctc acggcgctgg
     1381 cgctgaagca gggcggctac gactggggct atctggccta tgccgtggga ttcggcggct
     1441 cgatgatctg gttcggctct tccgccggtg tggcgttggc caacatgtat ccggaagcca
     1501 agtccgcggg acgctgggtc agccagggct ggcccgttgt cgcggcctat gtcatcggat
     1561 ttttcgtcat gcttgcggtg atcggctggc atcccgacgc gccgcattag ccgcggcgcg
     1621 ccctaccccg cccgcgccgc gcggcgcagc gattgcggcg gctgcccgaa ggcgcggatg
     1681 aaggcgcggc gcatccgctc cggatcgcga aagccggtga gttgcgcaac ccgttcgatc
     1741 gcttcgccgg aggattgcac gcgctcgcgc gccacctcga tccgcaaccg ctcgaccgcc
     1801 ttcgacggcg tggtgcctgt ctccgcgatg aaggcgcggg tgaaatgccg cgaactcatg
     1861 ccggcctgct cggccagatt ctcgaccgtg agcggcgcat cgagatgttc gcgcacccag
     1921 gagagcagcg gtccaaagcg gccgttcggc gccttcaatt ccagtagcga cgagaattgc
     1981 gactggccgc cgctgcggcg gttgtagagc acaagctggc gcgcggtctt ctgtgcgatc
     2041 tcgtcgccga aatcctcagt caccatcgcc agcgccagat cgatgccggc ggtgatcccg
     2101 gccgaactcc agatgttgcc gtcgcggacg aaaatccggt cgggctcgag tttcacctcg
     2161 ggataggtga tgagaaaatg ccgggtgcgg cgccaatgcg tggtggcgcg gcggccgtcg
     2221 agcaggccgg cctcggcaag gatgtaggcg ccggagcaga cgctggcgag gcggatgccg
     2281 cgtcgtgcca tggtccgcac gaagtcaagc gtcttgtcgc acctcgccgc ggcttcgacc
     2341 ccctcgcctc cggccaccag caaggtggtg atcgcgccgt acggttttaa gccgcgcgcg
     2401 agcatctcga cacccgatga gctgcgcacc ggccccggcg tcaccgcgag aaccttgatt
     2461 gacggggact ggccggcaaa gcgggcggcg atctcgaaca ccgaaaccgg accagcggcg
     2521 tccagcagtt gaaattgcgg aaacaccagc acgccgatca cgatgggcta tgtcctgaaa
     2581 tgagggaaat tagccatttc ggacatgatg cgatcatgcc agcatgcgca ggtcaagcaa
     2641 attttcggag gtcgtcatgc ccgagccgct gcagattggt cttttgatat ttccaaaagt
     2701 gacccagctc gatctcaacg ggccggtgca ggtgttttcc agcgtgcccg gcgccaaggt
     2761 gcaccttatc tggaaacgga tcgagccggt cacgagcgac tcggtgctga cgctcacgcc
     2821 gaccatcacc tttgccgact gcccgcagct cgacgtgatc tgcgtgccgg gcgggttcgg
     2881 caccgatgag atggtcaatg acgaggaggt gctgggtttc ctgcgccagc aggcgaaagg
     2941 cgcaaagtat atcacctcgg tgtgcacggg atcgctggtg ttgggcgcgg ccggcttgct
     3001 ccagggttat cgcgcgacga cgcactggtt ggcgatggac aatctcgccc cgttcggcgc
     3061 gacgccggca aagacccgcg tctgcgtcga ccgcaaccgc gtcaccggcg gcggcgtgac
     3121 cgcgggcatc gatttcgcgc tgacgctggt gtcgatcatg gtcgatcgcc agaccgcgga
     3181 ggcgatccag ctccggctcg aatataatcc ggcgccgccg ttcaacgccg gctcgcccga
     3241 caccgcgccg ccggaagtcc tggcgctgat gaaagagcga tatgcggcct cgcaggctcg
     3301 ccgtggcgag gcggtccggc gcgccgccgc gcggctggcg taaggtcgct gcaaccccag
     3361 tcaaagaaaa aggccgcctg gtttcccggg cggcctttcc tttcctacgg tggcttcaca
     3421 ttaacgggcg atttcagaac ctccgcacca ggggcctatc tcccggccgt gtcctactcg
     3481 gtagccgctg caaatcgggg cagtcgcgaa ctgttgcccg aaaccgacgc gatggtctcc
     3541 catctccgta agatgagcca ttgggccgcg attacggtca aagcacaagg acgcggggac
     3601 aaatgtcccc attgtccccg ttgttcacag acactgaccc gtcgttccgg ggcagcccga
     3661 cagggctgaa cccggaatct cgcgctgcat cctcgggatt ccgggttcgc gagctctgct
     3721 cgcgccccgg aatgacggcg gttgaatttc agctccccgg cttccagggc tgatagtcgc
     3781 cggtggc
//
LOCUS       DATNOI010000343         2251 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1805252,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000343 DATNOI010000000
VERSION     DATNOI010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805252"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..294
                     /locus_tag="VN968_13625"
     CDS             100..294
                     /locus_tag="VN968_13625"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HXL30313.1"
                     /translation="MDQPGQPALVKRYAGQRLYRPATSTYLTRGDLITMAKNGEKFVV
                     IDAHTHDDVTSLYHPIIVER"
     gene            300..494
                     /locus_tag="VN968_13630"
                     /pseudo
     CDS             300..494
                     /locus_tag="VN968_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
     gene            527..1120
                     /gene="phaR"
                     /locus_tag="VN968_13635"
     CDS             527..1120
                     /gene="phaR"
                     /locus_tag="VN968_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HXL30314.1"
                     /translation="MAKPEEPIVIKKYANRRLYNTGTSTYVTLEDLAGMVKNGEDFVV
                     YDAKSSEDITRSVLTQIIFEQENKAGQNLLPIAFLRQLIRFYGDSMQMLVPRYLEVSI
                     QSLAREQEKFRQHMSQAFGAGPFAPLEDQVRRNMEMFERAFTMFAPFARREAQAGKGD
                     QAAETEKPAAPAGEIDDLKRQLDDVQKRLDRLSETKG"
     gene            complement(1134..1544)
                     /locus_tag="VN968_13640"
     CDS             complement(1134..1544)
                     /locus_tag="VN968_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HXL30315.1"
                     /translation="MVTRDEAQRANVRAGRLRDAGPTAVSATYNRRSGRVVVRLTSGI
                     DVGFSPHDTQGLETAKPAQLDPIEISPSGLGLHFPKVDADLYLPALLEGVFGSRRWMA
                     ARLLREGRSRTAAKSTASRKNGGLGGRPRKTAPG"
     gene            complement(1617..1778)
                     /locus_tag="VN968_13645"
     CDS             complement(1617..1778)
                     /locus_tag="VN968_13645"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HXL30316.1"
                     /translation="MPTVLRFAGLRVVIYPNDHRPAHVHVIGKGCEAVFNLHCPRGPP
                     SCERTTVSI"
     gene            complement(1808..>2251)
                     /locus_tag="VN968_13650"
     CDS             complement(1808..>2251)
                     /locus_tag="VN968_13650"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXL30317.1"
                     /translation="SGIMLHNRGACFAVDPDHPNAIGPSKRPMNTIIPALGVRDGRCE
                     LAFGIMGAHFQPMGHAHLITNLIDYRMDLQAAIDQPRVFYEGEATIVERGISAAAIDG
                     LKQRGHKVAVRERPWGGAQAIAIDWQRGLLIGASDGRKDGCAMGY"
BASE COUNT          496 a          696 c          644 g          415 t
ORIGIN      
        1 gctgcccaca aaagtagtag cccattgtca tgaaacgtca tatttttgac gtggatgcaa
       61 aaaaactcta ccgagacatc accggatcaa acccacgcca tggaccagcc cggacagcca
      121 gcgcttgtta aaagatatgc aggacaacgc ctctatcgtc ctgcgacgag cacctatctg
      181 acccgcggcg atctcataac gatggcgaaa aacggcgaga aattcgttgt catcgatgcc
      241 catacccacg acgacgtcac atctttgtat caccccatca tcgtagagcg ttgaaggcca
      301 tgcccagacc cgaagagccg attgtcataa gaaagtaccc taatcggcgg ctctacaaca
      361 ccgagaccag cacttatatc acgctggaat acctcgccga catgatcaga cagggcgagg
      421 atttcgtcgt ctatgacgcc aaatcgggcg aggatatcac cggttccgtc atcacgcaga
      481 tcatcttcac gcagaccgtc cccaccatac attgagcact gaagccatgg ccaagcccga
      541 agagccgatc gtcattaaga agtacgccaa tcgccggctc tacaacaccg gaaccagcac
      601 ctacgtgacg ctggaggatc tcgccggcat ggtcaaaaac ggcgaggatt tcgtcgtcta
      661 cgacgccaaa tcgagcgagg atattacgcg ctccgtcctc acgcagatca tcttcgagca
      721 ggagaacaag gctggccaga accttctgcc gatcgcgttt ctgcgccagc tcattcgttt
      781 ttatggcgac agcatgcaga tgctggtgcc gcgttatctc gaagtttcga tccagtcgct
      841 cgcccgcgag caggagaagt ttcgccagca catgagccag gccttcggcg ctggtccgtt
      901 cgctccgctg gaggatcagg tgcggcggaa catggaaatg ttcgagcggg cgtttaccat
      961 gtttgcgccg ttcgcgcggc gcgaggccca ggccgggaag ggtgaccagg cggccgagac
     1021 tgaaaagccg gcggctccgg ctggcgaaat cgacgatctc aagcgccagc ttgatgacgt
     1081 gcagaagcgg cttgaccgcc tgagcgagac gaagggctga ttgggaaggc gggtcacccg
     1141 ggcgcagttt ttcgcggccg gcccccaagc ccaccgttct tacggctcgc ggtagatttg
     1201 gcagcagtgc gactgcggcc ttcccgtagt aaacgagcgg ccatccagcg cctagagccg
     1261 aagacccctt ccaacagcgc cggcagatag aggtcggcat cgactttcgg gaaatgcaac
     1321 ccgagaccgg acggactgat ttcgataggg tcaagctgcg ccggcttcgc ggtttccagc
     1381 ccctgcgtgt catgcggcga gaatccaacg tcgataccgc tggtcaatct cacgacaacg
     1441 cggccgctgc ggcggttgta cgtggccgag accgctgttg ggccggcatc acgcaagcgg
     1501 ccggccctaa cattggcccg ctgagcctca tcacgggtta ccatgcaatc tctcccattc
     1561 tcggcacata gcggcgaggt ctaaagccaa acctgtctcg atgcggttta ggtctctcag
     1621 attgaaaccg tagttctctc gcaactcggc ggtccacgag gacagtgcag attgaacacc
     1681 gcttcgcagc ccttaccaat aacgtgaacg tgcgccggcc gatgatcgtt cggataaatc
     1741 acgacgcgta gcccggcaaa acgcaacact gtgggcatag ggcaccctac gataaaccaa
     1801 tgatgtttca ataccccatc gcgcagccat ccttgcggcc gtccgatgca ccgatgagca
     1861 ggccgcgctg ccagtcgatg gcgatcgcct gggcgccgcc ccagggccgc tcccgcaccg
     1921 cgaccttatg accgcgctgc ttcagtccat cgatcgccgc cgctgaaatc ccgcgctcca
     1981 cgatggtggc ctcaccctcg tagaataccc gcggctggtc gatcgcggct tgcagatcca
     2041 tgcgatagtc gatgagatta gtgatgaggt gggcgtggcc catcggctgg aaatgcgcgc
     2101 ccatgatgcc gaaggcgagc tcgcagcggc cgtcccggac cccgagcgcc ggaatgatgg
     2161 tgttcatcgg tcgtttagac gggccgatcg cgttgggatg atcgggatcg acggcgaagc
     2221 aggcgccacg attgtgcagc atgatgccgc t
//
LOCUS       DATNOI010000344         3864 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1216122,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000344 DATNOI010000000
VERSION     DATNOI010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216122"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VN968_13655"
     CDS             complement(<1..646)
                     /locus_tag="VN968_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL30318.1"
                     /translation="MQEALRFLLPDAEPVERTDFLEQARLARRGPEVSRGLPLTIRGV
                     RKSFGANEVLRGIDLHIPAGQFVAIVGRSGCGKSTLLRLIAGLETIDAGTISFGEHAR
                     SEDVRVMFQEPRLLPWARVLSNVEVGLGRDRASSNAQIRAEKALTEVGLDDKRDQWPA
                     VLSGGQKQRVALARALVGDPRVLAFDEPLGALDALTRISMQRLLERVWHDQAFTA"
     gene            complement(655..1461)
                     /locus_tag="VN968_13660"
     CDS             complement(655..1461)
                     /locus_tag="VN968_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL30319.1"
                     /translation="MSLIESLPRVRSLWLPRADGLIQWIVPLVIVVVWQVACVSGFVP
                     ARVLPAPSDVALAGWKLLLSGELARNIWVSFWRASVGFVIGGGIGFAFGLMNGLSQLS
                     SKLTDTTLQMVRNIPHLALIPLVILWFGIDESAKLFLVALGVFFPIYLNTLHGIRTVD
                     PQLIEMGRIYGMTDGELFRRVIFPGALPSIFVGVRFALGIMWLTLIVAETIAASSGLG
                     YMAMQAREFMLIDVVVLSILIYALLGKLADSASRVLERLTLSWHPAFQKH"
     gene            complement(1458..2630)
                     /gene="ssuD"
                     /locus_tag="VN968_13665"
     CDS             complement(1458..2630)
                     /gene="ssuD"
                     /locus_tag="VN968_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HXL30320.1"
                     /translation="MSVHPSSNANILWFLPTHGDGRYLGTTTGGREVNFNYLRQIAQA
                     ADQLGYFGVLLPTGRSCEDSWVVASAVAPWTERLRYLVAVRPGLQSPGVAARMTATLD
                     RVSNGRLLVNVVTGGDPVENKGDGIFLDHDQRYAVTREFLNVYSDLLAGKTVNVEGKH
                     IRIEDGRLLFPPVQSPRPPLYFGGSSDAGIDVAVDTVDKYLTWGEPPAQVAEKVNRVK
                     AVADKRGRKLSFGIRLHVIVRETNAEAWKAADELIQHVTDETVASAQKIFSRMDSVGQ
                     QRMAQLHGGRRDKLEISPNLWAGVGLVRGGAGTALVGDPTTVAARIREYRDVGIDSFI
                     MSGYPHLEEAYRFAELVFPLLSLQQPNNVTPLRVNTGPFGETIANEHRPLVKASQS"
     gene            complement(2627..3577)
                     /locus_tag="VN968_13670"
     CDS             complement(2627..3577)
                     /locus_tag="VN968_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL30321.1"
                     /translation="MRWLERLVATAVLSMSIALAGVGASYGQDKVVRIGFQKYGKLVL
                     LKSKGTLEDKLKAAGYRVVWTEFPSGPPLLEALNVGAIDFGNTGEAPPIFAQAAGAPI
                     QYVAYEPPAPKGEAILVPKDSKLTSVADLRGKKVALNKGSNVHYLLVKALEKAGVKYS
                     EVEPVFLAPADARAAFERGAVDAWVIWDPFQAAAEAATGARTLADGTGVVSNYQFYFS
                     SKKFLQSASGIVDLVLAELREVDDWAKGDIHAVANQLAPAIGLPVDVVEVALKRQSYG
                     IKPITDSVIADQQQVADTFFALGLLPKQIKISDAARRPGT"
     gene            complement(3616..>3864)
                     /locus_tag="VN968_13675"
     CDS             complement(3616..>3864)
                     /locus_tag="VN968_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL30322.1"
                     /translation="VTSSTAKWAEAHRDEVAKSLSAVTGIALDIQTVAANRSSFVVGP
                     ITDDIVATQQGVADRFFKLGLIPKPVVVRDAVWKPAQT"
BASE COUNT          754 a         1264 c         1188 g          658 t
ORIGIN      
        1 tcgccgtaaa cgcctgatcg tgccagaccc gctccaatag ccgctgcatg gaaatccgcg
       61 tcagggcatc gagcgcgcca agcggttcgt caaacgccag aacgcgggga tcgccgacca
      121 gcgcacgcgc gagcgcgacg cgttgctttt gtccgccgga gaggacggcc ggccactgat
      181 cgcgcttgtc gtcaagaccg acttcggtga gcgccttttc agcccggatt tgcgcattcg
      241 acgatgcgcg gtcgcggcca agcccgacct cgacgttcga cagaacccgc gcccagggca
      301 gcagccgcgg ctcctgaaac atgacacgga catcctcgga acgcgcgtgt tcaccgaagc
      361 tgatggtgcc ggcgtcgatg gtctcgagcc cggcgatcaa acgcagcagc gtgctcttgc
      421 cgcaaccgct gcggccgaca atcgccacga actgtccggc gggaatgtgc agatcgatgc
      481 cgcgcagcac ttcgtttgca ccgaacgatt tgcgcacgcc gcgaatggtc agcggcagcc
      541 cgcgcgagac ttccggaccg cgccgtgcca gccgcgcctg ttcgaggaaa tccgtgcgct
      601 ccacgggctc ggcatccggc agaagaaaac gaagggcttc ctgcatgtct actctcaatg
      661 tttctggaat gccggatgcc aggacagtgt cagccgctcc agcacgcgcg aggcgctgtc
      721 ggccagcttt ccgagcaatg catagatcag gatgctgagc acgacgacgt cgatcagcat
      781 gaactcgcgc gcctgcatgg ccatatagcc aagccccgac gacgccgcga tggtttccgc
      841 cacgatcagc gtcaaccaca tgatgccgag cgcaaagcgc acgccgacga agatcgacgg
      901 cagcgcaccg ggaaagatca cccgccggaa caattcgcca tcggtcatgc cgtagatccg
      961 ccccatctcg atcagttgcg gatcgaccgt gcggatgccg tgcagcgtgt tgagatagat
     1021 cgggaagaac acaccgagcg cgaccagaaa cagttttgcc gattcatcga tgccgaacca
     1081 caggatgacc agcgggatca acgcgaggtg cggaatgttg cgcaccatct gaagcgtggt
     1141 gtcggtaagc ttgctgctga gctgcgacag gccattcatc aggccgaagg caaagccgat
     1201 gccgccgccg atgacaaagc cgacgctggc gcgccagaaa ctgacccaga tgttgcgcgc
     1261 caactcgccc gacagcagca gcttccagcc agcgagcgct acgtcgctcg gcgccggcaa
     1321 tacccgcgcc ggcacaaatc cgcttacgca ggcgacctgc cagacgacaa cgatgaccag
     1381 cggcacgatc cattggatca ggccatcggc ccgcggcagc cacaggctgc ggacacgcgg
     1441 gaggctttcg atcaggctca tgactgcgag gccttcacca acggccggtg ctcgttggcg
     1501 atggtctcgc cgaacggccc ggtattgacg cgaagcggcg tcacattgtt cggttgctgc
     1561 agtgacagca acggaaacac cagttcggcg aagcggtagg cttcctcgag atgcggatag
     1621 cccgacatga tgaagctatc gatgccgacg tcccgatatt cccggatgcg cgcggccacg
     1681 gtcgtgggat cgccaaccag tgccgttccc gcgccgccgc gcacgaggcc aacgccggcc
     1741 cagagattcg ggctgatctc gagcttgtcg cggcgtccgc catgcaattg cgccatgcgc
     1801 tgctggccga ccgagtccat ccgggaaaag atcttctgcg ccgaggcgac cgtctcgtcg
     1861 gtgacgtgct ggatcaattc atccgcggcc ttccaggctt ccgcattggt ttcgcgaacg
     1921 atcacatgca gacggatgcc gaaagagagc ttgcgtccgc gcttgtcggc aaccgccttc
     1981 acgcggttga ccttttcggc gacctgcgcc ggcggctcgc cccatgtcag gtatttgtcg
     2041 acggtatcga cggcaacatc gatgccggcg tccgacgatc cgccgaagta cagcggcggg
     2101 cgcggcgact gcaccggcgg gaacagcagg cggccatcct cgatgcggat gtgcttgcct
     2161 tccacattga ccgtcttgcc cgcaagcagg tcgctataga cattgagaaa ttcgcgcgtg
     2221 accgcatagc gctggtcatg atcgaggaaa atcccgtcac ccttgttttc gacgggatcg
     2281 ccgccggtaa cgacattgac cagcaaccgt ccattcgaca cccggtcgag tgtcgcggtc
     2341 atgcgcgcag cgacaccagg cgattgcagg ccggggcgca ccgccaccag ataacgcagc
     2401 cgctcggtcc atggcgccac cgcggatgcg accacccagg aatcctcgca gcttcgcccc
     2461 gtaggcaaca gcacgccgaa atagccgagt tgatccgcag cctgcgcgat ctggcgcaga
     2521 tagttgaagt tgacctcgcg tccgccggtg gtggtgccga gatagcggcc gtcgccatgg
     2581 gtcggcaaga accagagaat attggcgttc gatgacggat ggacactcat gtgccgggcc
     2641 tccgcgctgc gtcggaaatc ttgatttgtt tcggcagcag gccgagtgcg aagaaggtgt
     2701 ctgcgacctg ctgctgatcg gcgatcacgc tgtcggtgat cggcttgatg ccataggact
     2761 gtcgcttcaa ggccacttcg acgacatcaa ccggaagccc gatcgcaggc gcaagctggt
     2821 tggcgacggc atggatgtcg cccttggccc agtcatcgac ctcgcggagc tcggcgagca
     2881 ccagatcaac aatcccggaa gcgctctgca ggaatttctt cgaagagaaa tagaactggt
     2941 agttcgacac gacgccggtg ccgtcagcca gggtccgcgc gccggtggcg gcttcggcgg
     3001 cggcctggaa cggatcccag atgacccagg catcgaccgc gccgcgctcg aacgccgcgc
     3061 gggcgtccgc cggcgcgaga aacaccggct ctacttccga atatttgacg ccggcctttt
     3121 ccagcgcctt caccagcaga tagtgaacat tggagccctt gttcagcgcc accttcttgc
     3181 cccggagatc ggccaccgaa gtcagcttgc tatccttcgg caccaggatg gcctcgcctt
     3241 tcggcgccgg cggctcatag gcgacatact ggatcggtgc gcccgcagcc tgcgcgaaga
     3301 tcggcggcgc ctcgccggtg ttgccgaaat cgatcgcccc gacattgagc gcttcgagca
     3361 gcggcggacc ggacggaaac tccgtccaca ccaccctgta gccggcggcc ttcaatttat
     3421 cctccagcgt gcctttgctc ttgagcagca ccagcttgcc gtatttttga aagccgatac
     3481 ggaccacctt gtcttgaccg tagctggcgc cgaccccggc cagggctatg ctcatcgaca
     3541 gcaccgcggt cgcaaccagc cgttccaacc aacgcatgtt tcaatcctca ttgatgatgg
     3601 gtgatgccag gcggctcagg tctgcgccgg cttccagacc gcgtcgcgaa ccacgaccgg
     3661 ttttggaatg aggccaagct tgaagaagcg atcggccaca ccctgctggg tcgcgacgat
     3721 gtcatcggtg atcgggccaa cgacaaagga cgagcggttg gcggcgacgg tctggatgtc
     3781 caaggcaatg ccggtgacgg cgctcagcga ctttgccacc tcgtcgcggt gcgcctccgc
     3841 ccacttggcg gtcgaggacg tcac
//
LOCUS       DATNOI010000345        16176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_541044,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000345 DATNOI010000000
VERSION     DATNOI010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 16176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16176
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541044"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VN968_13680"
     CDS             <1..346
                     /locus_tag="VN968_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL30323.1"
                     /translation="AACGCLALLGIEPGTSYWEIGAQLTLMGAGLGLLVPPLTSTLLG
                     SVEKSRSGIAAGVLNSTRQTGSVLGVALFGSLAGQANAFVAGTHWSLIISACLLFAAG
                     AAIWFGASMKDS"
     gene            complement(385..819)
                     /locus_tag="VN968_13685"
     CDS             complement(385..819)
                     /locus_tag="VN968_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30324.1"
                     /translation="MAETTYFPRRLILAGAMISGVLLALAVHMLGARYGLDLGGLWRS
                     DTGEFMPAGAAVAWWLIATVGFSGGYFTATLMDSAVSGQIPQRMQHFLIGVGVLVLVG
                     AGQAAAAPSPIPTISGVLAGLAALCLGALVAFCGAHFALRKA"
     gene            919..1353
                     /locus_tag="VN968_13690"
     CDS             919..1353
                     /locus_tag="VN968_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HXL30325.1"
                     /translation="MTIGLLALAVAAIFTGAALYVGVAEQPARLLLDDRALLTEWKPS
                     YKRGAAMQASLALVGFLLGLIAWWQTSHSLFLIGSVAMIAPWPWTLIAIKPTNNALLA
                     TELERAGPPTRTLVVKWGALHAVRTALGALATSAYLWASLSR"
     gene            1361..2002
                     /locus_tag="VN968_13695"
     CDS             1361..2002
                     /locus_tag="VN968_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30326.1"
                     /translation="MRFASTGVYPEGSAHLPVTTDLPDETASNVCYAYKASLIGAAHR
                     FELTGDGLSWQVGGKSGVWAYADIAAIRLSYRPVSMQSRRFRADIESANRQNITVLST
                     SWQTVALMAPQDRDYRAFVTQLHRRMKAAGSTAALIGGVGPKTYAAGIVLLALVGIAI
                     SGLFIRAIATSEFSGALFLVGFAALFSWQIGGFMARNRPRAYTFDHLPAALLP"
     gene            2076..2690
                     /locus_tag="VN968_13700"
     CDS             2076..2690
                     /locus_tag="VN968_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HXL30327.1"
                     /translation="MQQACEADVEDRGTREPTEAEIAEISARHFVATPIKPADLLFVF
                     GTREDVAQRADEAFRLWREGLFRWSIVSGGVTPGATLSECAVIKTAMVERGIPPDRIL
                     EEHRAMNTGENVIFSLPVLEAALGLAHIRSVICLGNTWTARRYPMTLHRHWPEVEKML
                     ITVDSFETPPALWHTDPEFRTRVLWEWDKIEPYKARGFIAEWPE"
     gene            2687..3176
                     /locus_tag="VN968_13705"
                     /pseudo
     CDS             2687..3176
                     /locus_tag="VN968_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088389.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
     gene            complement(3547..3972)
                     /locus_tag="VN968_13710"
     CDS             complement(3547..3972)
                     /locus_tag="VN968_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXL30328.1"
                     /translation="MFKSILVPIDLSDTELAKPAIATAATLSQIWSGTVRLLNVLPMT
                     PVMLAEYVPADFDAQQREASEEALAIVARESGIDAQRISSVVRQGGIYHEVLEEAAAV
                     HADLIVMTSHRPAMRTYFLGSNAGHVVRYAKCSVLVVRH"
     gene            complement(4034..4573)
                     /locus_tag="VN968_13715"
     CDS             complement(4034..4573)
                     /locus_tag="VN968_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXL30329.1"
                     /translation="MRRLMLLRHAKTEHDAPSGRDRDRRLDERGRTDATEIGGWIGRH
                     PPFPDVVLVSPAIRARQTWEIAWEAMKKLSPQPQVELLPELYGAGLSQLLQTVHAASS
                     DPKILMLVGHNPGMHELALALTGSGEAAARKALADNLPTSGLAVFDFARGNWADVAFR
                     RGRLVLFVSPKRLKQTSGE"
     gene            4669..6120
                     /locus_tag="VN968_13720"
     CDS             4669..6120
                     /locus_tag="VN968_13720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXL30330.1"
                     /translation="MSETSLGMTARSDGADMRERPRLSFDLDVDICVVGAGLAGLTVA
                     LEAARLGASVAVLEGRHVGWNASGHQLGTVMPGFGLPIGELIARIGFEDSRELWSLSK
                     QGADYVRANATEELMPGIALSEGALEVSNVEAGEQLISRLQMLSEDFETDVEGWQVDR
                     VRDQLKTDHYFHGVYYPKAFQIDGRKYVHGLAALARRAGARIFEDTPVVSIDPSGIRK
                     RVVTPSARLRASHIVLAGNIHLGEPLRRLSETLLPVWRYAAVTAPLGERLGEVVTFQG
                     SVTDTDGVDHFRIVDGDRLMWASPETTWAARPERFAGPIARRIRTIFPRLGTTEIAEV
                     WGGAVGQTVHGMPQIGQLRRGLWVTSGFGRQGLNTSAMAGQLIARSILWGDERWRLFS
                     PFELVWAGGLTGRVAGHLVGMWARGSSAAAGALARHRERAQARERIREARLLEANRQA
                     GTRPPRRLTPPVHPRPVRPRPPVQTNPGEGEGM"
     gene            6410..6910
                     /gene="msrB"
                     /locus_tag="VN968_13725"
     CDS             6410..6910
                     /gene="msrB"
                     /locus_tag="VN968_13725"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXL30331.1"
                     /translation="MIDRRILLASVASLLGLAAFRWLRASPANAAEKFEIEKTDAEWR
                     AQLTPQQYEILRKQGTERPGSSPLLKEHRKGIFACAGCDLPLFSSETKFESGTGWPSF
                     YQPLDNAVGKTEDRTFGILRTEIHCRRCGGHLGHVFDDGPKPTGLRYCMDGFALVFHP
                     AAPSAT"
     gene            7105..8934
                     /locus_tag="VN968_13730"
     CDS             7105..8934
                     /locus_tag="VN968_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HXL30332.1"
                     /translation="MGIFDALNTAVGGLQAQSFALQNISGNIANASTTGYKGIATSFE
                     DLIPDATSPSRQVAGGVTAFAQATITTQGTVSVTTVATNMAINGDGFFSVQKATGTTD
                     NLPVFNGVTDYTRRGDFQVNANGNLVNGAGYYLMGVGVDPKTGNPLGNVPQVLQFQNN
                     FIPAQTTTSIKYAANLPSTPTTAVSSTAAAGTINALGGLNPFDFSVNPLPLGTPPAPF
                     GDNSVTGTAEVNQAATPVPITAATTLSGASPSNSLSAGFAVGDTITVDGTTLTFIAGA
                     VSAGNNIGVGDTVGNLLAKIGSITGVTPTLTGGAITLHTGTAADLAITTGGTSTAING
                     LAALGFSGGTVTLARTPAGTAGAGTVIGNDLQTFLNESISGGAVTAYDSTGTAVNMQL
                     RWAKTDSAALGGSHTNTWNLFYQSNPNATGSQTAWVNAGTNFTFGPTGVLTSPATPAI
                     TIPNVAVGNQSLGNLSFSMGSGTLTQFASTGGTVTINTITQNGFAAGQLQSVAINNNG
                     IVVGTFSNGQNLDLASVTLSHFNGTNYLKSLDGGAYAVTDQSGPAIIGASGSISGSSL
                     EGSNTDIASEFTKLIVTQQAYSANTKVITTANTMVQDLLNVLR"
     gene            8982..10859
                     /gene="flgK"
                     /locus_tag="VN968_13735"
     CDS             8982..10859
                     /gene="flgK"
                     /locus_tag="VN968_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HXL30333.1"
                     /translation="MSLGSALAIAMSGLRANQSALSIVSSNIANAQTPGYISEGINQV
                     EVQTGDSGASVIVTGVNRQLDQFIQSQLRTETSGGAYADQIASVLTQLQSVYGTPGAA
                     GSLESAFSNFTTALQSLSTTSGTSSSQIAAATAAQSLVQQLNATTQGIQTLRSNAEQD
                     LNISVGQANTAMSQIAQLNTRLQGLNPTDPAAATLMDQRDKAIDQLSKLMDIRVSSNG
                     NNQTSIYTTNGVELVGAQASKLSFNSQGTLNANSQWNSNPAKSSAGTITCTLANGATV
                     DMIATNSIGSGQIGADLTLRDKTLVQAQTQVDQLAASMASALSDKTTAGTAAPAALAP
                     KAGFDLDTSNVLPGNTINLSYTDAATHTLHNVTIVRVDDPTALPLSNAGGNPNNPVIG
                     VNFSGGMASVVAQLNTALGGANLQFSNPSGSTLRVVDSGTGSATVNAASVTTTATSLA
                     GGSVQLPLFTDGNSLYTGVITGAGSQLTGLAGRITVNSALLTNPSNFTVYSTSPPTNA
                     GDTTRSDFLYSQLTTGTFTYSPQTGLGSAASPFKGTISSYMQQFLSQQSNASTSATQL
                     QQGQDVVVNTLQQKMKATSGVNIDSEMANLIALQNSYAANAHVMSVVQTMMTSLMQVQ
                     L"
     gene            10870..12762
                     /locus_tag="VN968_13740"
     CDS             10870..12762
                     /locus_tag="VN968_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HXL30334.1"
                     /translation="MAINSVGFNASILGQSVLQLKNQLATLQQQLTTGTKSTTYAGMG
                     VNEGFAIAARAQLANISAFSDTMSNINTNIGIANTALQSMVDIGTTVLNSANSSSQAL
                     NSAGQSIAQQTATSQLSSMVGILNMQAGDRYLFSGSAINTPSVASADDIINGTTTQAG
                     LKQVISERAQADGTSGLGRLVLSSPTPTSVQVAEDVAGSPFGLKLSAVSSTLTGATIT
                     GPAGSPAAVSIDLGGTNPNNGDQVSFAFKLPDGTTESIQLTATTTNPPPTGSFLIDPP
                     PASSTTTTAANLKTALNTAIGTLANTSLVAASAVEAGNDFFNTAGTATGSVVNNKAAT
                     PVPITGATLLSGAAGTDSLATNFAAGDTITVNGTPITFVASGAVGGNQVNVTDSVQTL
                     LTKIDAISGTSTPSTISGGVIALHTDNAAGFAVTSSNPAAFGALGFGATATATQPPLR
                     VSGSPLSSATSLVSGTPANTISWYTGEAGQGSARATATARVDQSVTVQYGARANEAAI
                     RSQLQTLAVFAAVTTSTTNPNAAAQVSALSERVAQNLARQPGQQTIMDIQSDFATAQM
                     SMKNAASRQTQAKGMLQGIVDQAESVSPDQVATEILALQTNLQASYQTTSMLSQLTLT
                     KYLPVG"
     gene            12854..13639
                     /locus_tag="VN968_13745"
     CDS             12854..13639
                     /locus_tag="VN968_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30335.1"
                     /translation="MTDQIHLMVATPCFGGQVSSIYAASVFHLQRAARSRPNVDLTVL
                     MRDGDALITRARANLVTLFLDNPSATHLLFVDADIGFTPDQVFRLIESEADVVAGVYP
                     IKRVNWDKARRMLESNRSTSPAAALDYVLEIEDPDRVVAVNGFTRVRFAGTGFLMIRR
                     HVLERMCQHPAYAPLQFFEDHSLDALAGSPNRFALFECMIDPKTGTYLSEDFAFCKRW
                     TDIGGEIWADLESRLDHVGPSVFHGDISSQFAAPASVRAANAA"
     gene            13644..15287
                     /locus_tag="VN968_13750"
     CDS             13644..15287
                     /locus_tag="VN968_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30336.1"
                     /translation="MPTLSTTPRLNAAEDPIARDRSGSAAAMAELFDPLDRLLSGGGD
                     QRLAIDPASGVNGYGCQPFPCPETLSFASSTATSISERAYDVASDARQSLMQSAIAVG
                     IDAAFDARIEAMRDELKACLGLSETGVEVVFSPSGTDSQLQALLLAGALLGPVLMTII
                     VAADQTGSGTADTARGHHFSVATAHGYRVRKGEPVAGLTHSVASVALPSFDETGGFRP
                     HADGDARVLGAIEKSVANGTNVLLQIMDSSKLGWRVPSDQCLDEIRARWPDRVQVVVD
                     ACQMRLGRPRLQHYLDRGYIVIVTGSKFFTGPAFSGALLVPAGLSKALGAITEVASGF
                     FEYFSRSDWPKNWPALRSCFPVRANLGQWLRWEAALEEIRAYYSVPNQFRLMALTMFG
                     KGVQRIIASSPSLRLLPLQQRREDGSVDDEELARTTIFPFLVRRGGGFFSLGDCRTIH
                     RALGRDLRNGVPANKHRRPEIAAKLCLVGQPVAVGHFAALRICASARLVTETWSSDQQ
                     AAHENVRRELGRVGTIVSKIEWLVAHMGGLNLTEACHEA"
     gene            15277..>16176
                     /locus_tag="VN968_13755"
     CDS             15277..>16176
                     /locus_tag="VN968_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30337.1"
                     /translation="MKLECTEEAGVVRYPCADRIGFARLTTMAFHGTDLRPLRDELIS
                     KVAGGTADAGEGLDLSLIAQLLGDKQTGLAIQAEVLAFHRLFRSPCSAEKPKLRVLAL
                     AAAIDMGGNTPIEFLLEDSGIELLTLYVIAGAELPVPLPDHDIAIVIASDSEECRDAL
                     RIIDRAATRWPRPILNLPRLVGNLDRDKLHGLLKGIEGLDIPATASVSRAQLSDVARS
                     KLLFADIAAEPRFPIIVRPRGSHAGVGLAKLDDHAAMDRYLAERPESEFFVSRFVDYA
                     NDDGLFRKYRIVFVDGRPYACHMA"
BASE COUNT         2957 a         5199 c         5015 g         3005 t
ORIGIN      
        1 ggcggcatgc ggatgcctgg cgctgctcgg gatcgaaccc ggcaccagct actgggaaat
       61 cggcgcgcaa ctgaccctca tgggcgccgg ccttggcctg ctggtgccgc cgctgacgtc
      121 aaccctcctg ggaagcgtcg agaaatcgcg ctccggcatc gccgccggcg tactcaactc
      181 gacccggcaa accggcagcg tgctgggtgt cgcgctgttc ggctcgctgg ccgggcaggc
      241 caacgccttc gtggcgggca cgcattggtc gctgatcatc tcggcgtgcc tgctgtttgc
      301 ggccggagca gcgatctggt tcggcgcttc catgaaagat tcgtagtgat cgagggcggc
      361 cggctcaagc gcgagcgcgc cggctcacgc cttgcgcagg gcaaaatgtg cgccacaaaa
      421 tgccaccagc gcgccgaggc acagcgccgc gagacctgcg agcacgcccg atatcgtcgg
      481 gatcggactg ggcgctgcgg ccgcctggcc cgcgcccacc aaaaccagca ctccgacgcc
      541 gatcaggaaa tgctgcatcc gctgcggtat ttgccccgac accgcgctgt ccatcagtgt
      601 cgcggtgaaa tagccgccgg agaacccgac cgttgcgatc agccaccacg ccacggccgc
      661 gcctgcgggc atgaattcgc cggtgtcgga tcgccacagg ccgccgaggt cgaggccgta
      721 gcgcgccccg agcatgtgca cggcgagcgc cagcagcact cccgaaatca tcgcgccggc
      781 gaggatcaga cggcgcggaa aataagtcgt ctcggccatg gcccgcttgt aaggtaaagc
      841 cggctcgccg cgcaagccgg ggcagagagt tcaggcaatc ttttgatcaa gcaatcgctt
      901 ttaggaaagg ctcggcacat gacgatcggt cttctcgcgc tggctgtcgc cgcgatcttc
      961 accggcgccg cgctttacgt tggtgtcgcc gagcagccgg cgcggctgct gctcgacgac
     1021 cgcgcgctgc tcaccgaatg gaagccgtcc tacaagcgcg gggccgcaat gcaggcgtcg
     1081 ctcgcgctgg tgggttttct tttggggctg atcgcgtggt ggcagacctc gcattccctg
     1141 ttcttgatcg gcagcgttgc gatgatcgcg ccgtggccat ggacgctgat tgcgatcaag
     1201 cccaccaaca atgcgctgct ggcgaccgag ctcgagaggg cggggccgcc aacgcgcacg
     1261 cttgtcgtca aatggggcgc actgcatgcc gtgcgcaccg cattgggcgc gctcgcaaca
     1321 tctgcttatt tgtgggcaag tctatcgcgc tagccgggac atgcgattcg cgagcaccgg
     1381 cgtctatccc gagggatcag cacatttgcc tgtgacgacc gatttgccgg acgagactgc
     1441 cagcaacgtc tgctacgcct acaaggcgtc gctgatcggc gcggcgcatc ggttcgagct
     1501 gaccggcgat ggattgtcgt ggcaggtcgg cggcaagtca ggggtctggg cctatgccga
     1561 tatcgccgcg atccgtctgt cctaccggcc ggtgtcgatg cagtcgcggc ggtttcgcgc
     1621 tgatatcgaa tctgcgaacc gtcagaacat cactgtcctc tcgaccagct ggcagaccgt
     1681 cgccttgatg gcgccgcagg atcgggatta ccgcgccttc gtcacgcaac tgcatcggcg
     1741 catgaaggcg gccgggagca cggccgccct gatcggcggc gtcgggccga aaacctatgc
     1801 cgccggcatc gtgttgctgg cgctggtcgg gatcgcgata tcgggactct ttattcgcgc
     1861 catcgccacc agtgaatttt cgggcgcgtt gtttctggtc ggcttcgccg cgctgttttc
     1921 gtggcagatc ggcggcttta tggcgcgcaa caggccgcgg gcctacacgt tcgatcatct
     1981 gccggcggcg ctgttgccgt aatcttggct ctgcaaaaaa gcgcaacgaa acgtgacttc
     2041 gcgtcgcggc cggcgcgccg cattgtcggt acctgatgca gcaggcatgc gaggcggacg
     2101 tggaggatcg aggcacgcga gagcctaccg aggccgagat cgccgagatc agcgccaggc
     2161 attttgtcgc gacgccgatc aagcccgccg atctgctgtt cgtgttcggc acccgcgagg
     2221 atgtggcgca gcgtgcggat gaagctttca ggctttggcg cgagggcttg tttcgctggt
     2281 cgatcgtcag cggcggcgtg acgccgggcg cgacactatc ggaatgcgcg gtcatcaaga
     2341 ccgcgatggt tgagcgaggc attccgcctg accgcatcct ggaagagcat cgcgcgatga
     2401 acaccggcga aaacgtcatc ttctcgctgc cggtcctcga agccgccctg ggcttggcgc
     2461 atatccgcag cgttatctgc ctcggcaaca cctggaccgc gcgccgctat ccaatgacgc
     2521 tgcaccggca ctggccggag gtggagaaga tgctgatcac ggtcgacagt ttcgagacgc
     2581 cgccggcgct ctggcatacc gatcctgaat ttcgaacccg ggtgctctgg gaatgggaca
     2641 agatcgagcc ctacaaggcc aggggcttta tcgcggagtg gccggagtga acgggccgag
     2701 gggtgatcgc ctccagcgct tctatttttt caccgaccgc gccttcggcg gcaatccgct
     2761 ttgcggtggt gctggacgcc ggcgggcgtt ccaccgcgca gatgcaggcc attgcggtcg
     2821 agttcaatta ctccgagacc accttcgtgt tgccgccgcg cgatgccgcg caggtgcgta
     2881 tctttaccgt gaccaccgaa atcccgttcg cgggacatcc caatgtcggt actgccttcg
     2941 tgctggcgac gcaggctgca aaaccgccgg cgcggctgaa gttcgaggaa ggcgccggcc
     3001 tggtgcccgt cgaaatcctg atggaaggcg gcagggtggt cggcgccgaa ctcaccgcgc
     3061 cgcaaccgct cagccgactg acggagttca gcgtcgagca ggcggcggcc acggtagcgc
     3121 tgtcggccgc ggatatcagc acagaccggc atccagccat gatcgtctcg gtcggaatgt
     3181 gaaggccgag gtcgccatca acgcaaccct cgtcattccc cgatgtgcaa ttgcacgcac
     3241 atccgaggat gcgccggttg gcgcaccccg tcgcgcttct cgatgcgccg acatccacca
     3301 cggcctgaac cagacgctct cgaccgaccg gcgtcattcg ggcattcttg tgcatgttca
     3361 tccggtccct ccctggattc tgaagcttcg caacctcagt ctcctcggtc cggatcggat
     3421 ggacaaccta ttgagagctc acatctagag ccttttccgt ttcgatagaa tcgaaacggg
     3481 gctctagctt cttagtttga cgcgttttct tgacgcgaac cggtttccac ttcgctcgaa
     3541 aacgctctag tgccggacca ccagcaccga gcatttggcg tagcgcacca catggccggc
     3601 gttggaaccg agaaaatagg tccgcatcgc cggccggtgc gatgtcatga cgatcaggtc
     3661 ggcatgaaca gccgcggcct cttcgagaac ctcgtgatag atgccgccct ggcgcaccac
     3721 gctggagatg cgctgggcat cgatgccgga ttcgcgggcc acgatggcga gtgcctcctc
     3781 ggaggcctcg cgctgctggg catcgaaatc ggccggcaca tattccgcca gcatcaccgg
     3841 cgtcatcggc aacacattga gaagccgcac cgtgccgctc cagatctgcg atagcgtcgc
     3901 tgccgtcgca atcgcgggtt tcgccagttc ggtgtcggaa agatcgatcg gcacgagaat
     3961 ggatttgaac atctgcgcct ccctgtgaag ccaacatagc ataaaacccg gttgacgcgg
     4021 cagatggttc tgcttactcg ccggaggttt gcttgagccg cttcgggctg acgaacagca
     4081 ccagccgtcc gcgacggaag gccacgtcgg cccagttgcc gcgtgcaaag tcgaataccg
     4141 caagccccga tgtcggcagg ttgtcggcga gcgccttgcg ggccgcggct tcaccgctgc
     4201 cggtaagcgc aagcgccaat tcatgcattc cgggattgtg cccgaccagc atcaggattt
     4261 tgggatcgga tgatgcggcg tggacggttt gcagcagttg cgacaggccg gctccgtaaa
     4321 gctcaggcag aagttcaacc tgcggctggg ggctcaattt tttcatcgcc tcccaggcaa
     4381 tctcccaggt ctggcgagcc cggatcgcgg gcgaaaccag caccacatcg ggaaaaggcg
     4441 ggtgccggcc gatccagccg ccgatctccg tggcgtcggt gcggccgcgt tcgtccaatc
     4501 ggcggtcccg atcgcggccg ctcggcgcgt catgctcggt tttggcgtgg cgcagcagca
     4561 tcaaacggcg catggcgatc tcgattcgtt tcggggcttg cggattaatg tacaagcaaa
     4621 agctcaggac aaggtgacaa gcgactgatc cgtccgtaag aaaacggtat gagtgagact
     4681 tctttaggca tgaccgcaag atccgatggc gccgacatgc gagagcgccc gcgcctttcg
     4741 ttcgatctgg acgtcgatat ctgtgtggtg ggagcggggc ttgccggcct cacggtggcg
     4801 ctggaggccg caaggctcgg cgccagcgtc gccgttctcg aaggccggca tgtcggctgg
     4861 aacgcatccg gccatcaact cggcacggtg atgccgggct ttggtcttcc gatcggcgaa
     4921 ctgatcgccc gcatcggctt tgaagacagc cgcgaactct ggtcattgtc gaagcagggc
     4981 gccgattatg ttcgggccaa cgccaccgaa gagctgatgc cgggcattgc gctgagcgag
     5041 ggggcgctgg aggtttccaa tgtcgaggcc ggcgaacaat taatcagccg gctgcaaatg
     5101 ctgagcgagg atttcgagac ggacgtggaa ggctggcagg tcgatcgcgt gcgcgatcag
     5161 ctcaagaccg atcattactt ccacggcgtg tattatccca aggcgtttca gatcgacggc
     5221 cgcaaatacg tccacggtct tgccgcgctg gcgcggcggg cgggggcgcg gattttcgag
     5281 gacacgccgg tcgtcagcat cgatccctcg ggcattcgca agcgcgtcgt cacgccgtcg
     5341 gcgcggttgc gtgcgtcgca catcgtgctc gccggcaaca tccatctggg cgagccattg
     5401 cggcggttgt cggagaccct gctgccggtc tggcgttacg ccgcggtgac cgcaccgctc
     5461 ggcgagcgcc tcggcgaggt ggtgacgttt cagggatcgg tgaccgacac cgacggcgtc
     5521 gatcatttcc ggatcgtgga cggcgaccgc ctgatgtggg ccagcccgga gaccacctgg
     5581 gcagctagac cggagcgttt tgccggcccc attgcgcgca ggatccgcac gatctttccg
     5641 agactcggaa caaccgagat tgccgaagtc tggggcggtg ccgtcggcca gaccgtgcac
     5701 ggcatgccgc agatcggcca gttgcgtcgc ggtctgtggg tcacgagcgg cttcggtcgc
     5761 cagggcttga acacctcggc gatggccggg cagttgatcg cgcgcagcat tctgtggggc
     5821 gacgagcgct ggcggctgtt ttcgccgttc gaactggtgt gggcgggggg cctgaccggc
     5881 cgggtagccg gacatctcgt cgggatgtgg gcgcggggca gttccgccgc cgccggcgcg
     5941 ctggcccgtc atcgggagag agcacaggcc cgagaacgca ttcgcgaggc gcgtctgctc
     6001 gaggccaatc gccaggccgg gaccagaccg ccacggcgcc tgacgccgcc ggtccacccg
     6061 cgtcccgtgc gtccccggcc gcccgtccag acaaaccctg gcgagggtga ggggatgtag
     6121 gcgcgtttgc gtgttccgga cgcgtgcagc gttctcccgg cgacgccaac gggtcgcgcg
     6181 aatgcgcgcc cgatgacagg ctccgcgtcg tccggttacg ctgctccgca gagcggggac
     6241 ccatcgggaa ggtgtttggg gcctggatca gcggcgcaat tgctgtgcag catccgaggg
     6301 catctcccaa caaagtgaga aaattccctc ccaatcggtg taacttctgc ctccgcggcc
     6361 ccgtatctag cggcgaacga caccgaattc gcttcgaacg gagaccatca tgatcgaccg
     6421 ccgtattctg ctggcgtctg tcgctagttt gcttggcctt gcggccttcc ggtggctgcg
     6481 cgcttcgcct gccaacgccg cggagaagtt cgagatcgaa aagaccgatg cggaatggcg
     6541 cgcgcagttg acgccgcagc aatatgagat tctgcgcaag caaggcaccg agcggcccgg
     6601 atcgagcccg ctgctcaagg agcaccgcaa gggcatcttc gcctgtgccg gctgcgacct
     6661 gccgctgttc tcctcggaga cgaaattcga gagcggcacc ggctggccga gtttttacca
     6721 gccgctcgac aacgccgtcg gcaagaccga agaccggact ttcgggatat tgcggaccga
     6781 aattcactgc cgccgctgcg gcggccatct cggtcacgtc ttcgacgatg ggccgaagcc
     6841 gaccggcctg cgctactgca tggatggctt tgccctggta ttccatccgg cagccccctc
     6901 ggcgacctga tccgtcgcct gcgcccggca attgttgccc cgctcggcaa ttgtgccccg
     6961 gtcattggac cggggatttg tttttaaggc attgatattg ttataatttc cttgttggca
     7021 caacgcttgc gaaggtctcc ttcgatagcg gtcatggctt tgcaaaccaa ccggccgcgc
     7081 ggatcgaatt tggagacgat agttatgggt attttcgacg cgctcaatac cgcggttggt
     7141 ggccttcagg cgcaatcctt cgccttgcag aacatctccg gcaatatcgc gaatgcttcg
     7201 accacgggct acaagggcat cgcgaccagc ttcgaggatc tcattccgga tgcgacgtcg
     7261 ccgagcaggc aggtcgcggg cggcgtgacc gcctttgcgc aagccacgat caccacccag
     7321 ggcaccgtct cggtcacgac cgtcgcgacc aacatggcga tcaacggcga cggttttttc
     7381 tccgtgcaaa aggccaccgg aaccaccgac aaccttcctg tttttaacgg cgttaccgac
     7441 tatacccgcc gcggcgattt tcaggtcaat gccaacggca atctggtcaa cggcgccgga
     7501 tattacctga tgggtgtggg ggtcgacccg aagaccggca atccgctcgg caacgtgccc
     7561 caggtgcttc agttccagaa caacttcatt ccggcgcaga ccaccacttc aatcaagtat
     7621 gccgccaacc tgccgtccac cccgacgaca gccgtcagct cgaccgcggc ggccggcacg
     7681 atcaatgccc tcggcggcct caatccgttt gatttcagtg tcaatccctt gccgctcggc
     7741 acgccgcccg caccgttcgg cgacaattcg gttaccggca ccgccgaggt caaccaggca
     7801 gcaacgccgg tcccgatcac cgccgcgacc accctatccg gcgcgtctcc aagcaattcg
     7861 ctgtcggcgg gcttcgcggt cggcgacacc atcacggtcg acggcacgac attaaccttc
     7921 atcgccggcg cggtcagtgc cggcaacaac atcggcgttg gcgacacggt cggcaatctg
     7981 ctcgcgaaga tcggctcgat aaccggcgtg acgcccaccc tcaccggagg tgcgattacg
     8041 ctgcacacgg gcacagcagc cgatctggca attaccactg gcggtacctc caccgcgatc
     8101 aacgggctcg cagccctcgg tttttccggg ggaaccgtga cgctggcgcg aaccccggcc
     8161 ggtacggcgg gcgcgggaac ggtcatcggc aacgacctcc agaccttcct gaacgagtcg
     8221 atcagcggcg gcgccgtcac cgcctacgat tcgaccggaa cggcggtcaa tatgcagctg
     8281 cgatgggcca agaccgacag cgcggccctc ggtggctcgc acaccaatac gtggaacctg
     8341 ttctatcaaa gcaatccgaa cgccacgggc tcgcagaccg cctgggtcaa tgccggaacc
     8401 aacttcacgt tcggaccaac gggagtgctg acgagtccgg caactcccgc gatcaccatc
     8461 cccaatgtcg ccgtcggaaa ccagtcgctc ggaaacctgt cgttcagcat gggttccggc
     8521 actctgaccc aatttgccag caccggcggc accgtgacga tcaacaccat cacgcagaac
     8581 ggcttcgccg ccggccagct tcaatcggtc gccatcaaca ataacggcat cgtcgtcggc
     8641 acgttttcca acgggcaaaa tctcgatctc gcctcggtca cgctgtcgca cttcaacggc
     8701 accaattacc tcaagagcct cgatggcggc gcctacgccg tcaccgacca atcgggacct
     8761 gcaattatcg gcgcctcggg cagcatcagc ggctcgtcgc tcgaagggtc gaacaccgat
     8821 attgcttccg aattcaccaa gctgatcgtg acccagcagg cctattccgc gaataccaag
     8881 gtgatcacca cggccaacac catggttcag gatctcctga acgtgctgcg ctgatagcca
     8941 tgacggcaac ggggcaattc ggattgatct gaggcggcat tatgagtttg ggttctgctc
     9001 tcgccattgc gatgtccggt ttgcgtgcaa accagtccgc gctttcgatt gtgtcgtcga
     9061 atatcgcgaa cgcgcagaca cccggttaca tctccgaagg catcaatcag gtcgaggtgc
     9121 aaaccggcga cagcggtgcg agcgtcatcg tcaccggcgt caaccgccag ctcgatcaat
     9181 tcatacagtc tcagttgcgg accgaaactt ccggcggcgc ctatgccgat cagatagcga
     9241 gcgtactgac ccagctgcaa tcggtctacg gaacacccgg agctgccggt tcgctcgaaa
     9301 gcgccttttc caattttacc accgcgcttc aatcgctctc cacgacttcg gggacttcat
     9361 cgtcacagat cgcagccgcg accgcggcgc aatcgctggt tcagcaactg aatgccacga
     9421 cccagggcat tcaaacgctg cgttccaacg ccgagcagga cctcaatatt tcggtcggcc
     9481 aggccaacac ggcaatgagc cagatcgcgc aactcaacac ccggcttcag gggttgaacc
     9541 cgacagatcc ggccgcggcg acgctgatgg accagcgcga caaggcgatc gaccagctgt
     9601 cgaaactgat ggatatccgc gtctccagca acggcaacaa ccagacatcg atctacacga
     9661 ccaacggcgt tgagcttgtc ggcgcccagg catccaagct cagtttcaac tcgcagggga
     9721 cgctgaacgc caattcgcag tggaacagca atccggccaa atcctccgcc ggcaccatca
     9781 cgtgcacgct cgcgaacggc gccaccgtcg atatgatcgc gaccaactcg atcggttccg
     9841 gccagatcgg ggccgatctg acgctgcgcg acaagaccct ggttcaggcg cagacgcagg
     9901 tcgatcagct ggcggcgtcg atggcaagcg cgctgtcgga caagaccacc gccggcaccg
     9961 cggcccccgc cgcgctggcg ccgaaggccg gattcgatct cgatacgtcg aacgtgctgc
    10021 ccggcaatac gatcaatctc agctataccg acgccgcgac ccataccctg cacaacgtca
    10081 cgatcgtgcg tgtcgacgat cccacagcac ttccgttgtc gaatgccggc ggaaatccca
    10141 ataatccggt gatcggcgtc aatttttcgg gcggcatggc gtcggtcgtg gcgcaactga
    10201 acaccgctct tggcggcgcc aatttgcagt tttccaatcc gtccggctcg accttgcgcg
    10261 tcgtcgacag cggcaccggc tctgcgaccg tcaacgcggc ttcggtcacg accaccgcca
    10321 catcgcttgc gggcgggagc gttcagttac cgttgttcac agatggcaat tcgctctaca
    10381 ccggcgtgat caccggcgcc ggatcgcaat tgaccggtct cgcgggacgc atcaccgtca
    10441 actcggcgct gctcaccaat ccgtcaaact tcaccgtcta cagcacctcg ccgccgacca
    10501 acgccggcga cacgacgcgt tcggattttc tctactccca gctgacgacc ggcaccttca
    10561 cttactcgcc tcagacagga ctaggctcgg ccgcgtcgcc gttcaaggga acgatctcca
    10621 gctatatgca gcagttcctg agccagcaga gcaatgcgtc tacgtccgcg acccagcttc
    10681 agcagggtca ggacgtcgtc gtcaacacgc tgcagcagaa aatgaaggcc acctccggcg
    10741 tcaacatcga tagcgagatg gcgaacctga ttgcgctgca gaattcctat gcggccaatg
    10801 cgcatgtgat gtctgtggtt cagaccatga tgaccagctt gatgcaggtc cagttgtaag
    10861 gcgatcgata tggcaatcaa tagcgtcggt tttaatgcct ccattctcgg ccagtcggtg
    10921 ctgcagctca agaatcagct ggcgacgctt cagcagcagc tgacgaccgg gacaaaatcg
    10981 acgacctatg cggggatggg cgtcaatgag ggattcgcca tcgcggcacg cgcgcagctg
    11041 gcgaacattt ccgcgttcag cgataccatg agcaacatca ataccaacat cggcatcgcc
    11101 aacaccgcac tgcagtcgat ggttgatatc ggcacgacgg tgctcaacag cgcgaacagt
    11161 tcgtcgcagg cgttgaacag cgccggacag tccatcgccc agcaaaccgc gacgtcccag
    11221 ctgtcctcga tggtcggcat cctcaatatg caggcgggtg accgctacct gttttcgggc
    11281 agcgccatca atacgccatc ggtcgcctcg gctgacgata tcatcaatgg gaccaccacc
    11341 caggccggat tgaagcaggt gatttcggag cgcgcgcagg ccgatggcac cagcggtctg
    11401 gggcgtctcg ttctctcttc gccgacgccg acctcggtgc aagtggcgga ggatgtcgcc
    11461 ggctcgccgt tcggcctgaa gttgagtgcg gtctcgtcga cgctgacggg ggcgaccata
    11521 accggccctg cgggatcgcc agccgcggtt tcgattgacc tcggcggcac caacccgaac
    11581 aacggcgatc aggtgagctt tgcgttcaag ctgccggatg gcacgactga atcgattcag
    11641 ttaacggcaa ccaccacaaa tcctccgccg accggaagtt ttttgatcga cccgcccccc
    11701 gccagttcga cgacgacgac ggcggccaac ctgaaaacgg cgctgaacac ggcgatcggc
    11761 acgctcgcca atacctcgct ggtggccgcg tcggcggtcg aggccggcaa cgatttcttc
    11821 aatacggccg gaacggcaac gggaagcgtg gtcaacaaca aggctgcgac ccccgtgccg
    11881 atcaccggcg cgacgctgtt gtccggtgcc gcgggcacgg actcgctggc caccaacttc
    11941 gcggccggcg acaccatcac cgtcaacggc acgcccatca cgtttgtcgc ctccggcgcg
    12001 gtgggcggca accaggtcaa cgtcaccgac agcgtccaga cgctgttgac gaaaatcgat
    12061 gcgatcagcg gcacttcgac tccatcgacc atcagcggcg gcgtgatcgc gctgcacacc
    12121 gataacgccg cgggcttcgc ggtcacgagc tcgaatccgg ccgcgttcgg ggcgctcggc
    12181 ttcggcgcca ccgcgacggc gacgcagccg ccgctgcggg tcagcggctc cccgttgagt
    12241 tcggcgacgt cgctggtcag cggcacgccg gccaacacga tttcctggta caccggcgaa
    12301 gccggtcagg gttccgcccg cgcaaccgcg acggctcgcg tcgatcagtc ggtgaccgtg
    12361 cagtacgggg cgcgcgccaa cgaggcagcc attcgttcgc agcttcaaac gctggcggtt
    12421 ttcgccgcgg tcacgacctc gacgacaaat ccaaacgccg ccgcgcaggt ctcggcactc
    12481 agcgagcggg ttgctcaaaa tcttgcgcgg cagcccggcc agcagaccat catggatatc
    12541 cagtcggatt ttgcgacggc gcagatgtcg atgaaaaacg ccgcgtcccg gcaaacccag
    12601 gccaagggca tgctgcaggg catcgtcgac caggccgaga gcgtttctcc cgatcaggtg
    12661 gccaccgaga tattggcgct ccagaccaac ctgcaggcgt cctaccaaac cacctccatg
    12721 ctgtctcagc tcaccctcac caagtacctt ccggtcggct aacgccgggt agcggccgtt
    12781 aggctttcgt taactacgtc tatttactgt ttggtgagaa gtacaggcaa tgtcagcggc
    12841 accggagcca ttcatgaccg accagattca cctgatggtg gccacgccct gtttcggcgg
    12901 gcaggtctcc agtatttacg ccgcctcggt cttccatttg cagagggccg cgcgttccag
    12961 gccgaacgtg gacctcacgg ttctgatgcg cgacggcgac gcgctgatta cccgtgcgcg
    13021 agccaatctg gtgacgctgt tcctcgacaa cccctcggcg acccacctgt tgttcgtgga
    13081 tgccgatatc ggcttcacgc ccgaccaggt attccggctg atcgaatccg aagccgacgt
    13141 ggtggccggt gtctatccca tcaagcgggt caactgggac aaggccaggc ggatgctgga
    13201 gtccaacagg tcgacatcgc cggcggccgc gctcgactat gtgctggaga tcgaggatcc
    13261 ggatcgcgtc gtcgccgtca atgggtttac ccgggtgcgc tttgccggca ccggcttcct
    13321 gatgatcagg cgtcacgtcc ttgaaaggat gtgccagcat ccggcttacg cgccactgca
    13381 gttcttcgag gaccattctc tcgatgcgct cgccggaagc cctaaccggt ttgcgctgtt
    13441 cgagtgcatg atcgatccga agaccggaac ttatctcagc gaggattttg cgttctgcaa
    13501 acgctggacc gatatcggcg gcgaaatctg ggctgatctt gagagccgcc tcgatcatgt
    13561 cggcccgtcg gtgtttcatg gcgatatctc gtcgcagttt gccgcgcctg catccgtaag
    13621 agcagcaaac gccgcgtgaa atcatgccga cgttgagcac cacgcctcgg ttgaatgcgg
    13681 ccgaggatcc gatcgctcgg gatcggtccg gttcggccgc cgccatggcc gagctgttcg
    13741 atccgctgga ccgcttgctc agcggcggcg gcgatcagcg gctggcgatc gatccggcaa
    13801 gcggggtcaa cggatatggc tgccagccct ttccttgtcc ggaaacattg agctttgcct
    13861 caagcacagc gacatcgatc tcggagcggg cttacgacgt tgcgagtgat gcgcggcaat
    13921 cgttgatgca gtccgccatt gccgtcggca tcgatgccgc gttcgatgcc cgcatcgagg
    13981 cgatgcgcga cgaattgaag gcctgtctcg gactgtccga aaccggggtc gaggtcgtct
    14041 tttctccgtc gggcaccgac tcgcagttgc aggcgctgtt gctcgcgggg gcgctgctcg
    14101 gccccgtgtt gatgacgatc attgtggccg ccgatcaaac cggaagcgga accgcggata
    14161 ccgcgcgcgg gcatcatttc agcgtcgcga ccgcgcacgg atatcgggtt cgcaagggag
    14221 agcccgttgc cggcctgacg cattccgtcg caagcgtcgc gctgccatcg ttcgatgaga
    14281 ccggcggctt ccgcccgcac gccgacggcg atgcacgggt tctcggcgcg atcgaaaagt
    14341 cggtcgcgaa cggaaccaat gtcctgcttc aaatcatgga ttcgtcgaag ctgggctggc
    14401 gggtgccaag cgaccaatgt ctcgacgaaa tcagggcgcg ctggcccgat cgggtccagg
    14461 tcgtcgtcga tgcctgtcaa atgcgccttg gtcggccgcg gctgcaacac tatcttgacc
    14521 gtggatacat cgtgatcgtc accggctcga agtttttcac cgggcctgcc ttcagcggcg
    14581 cgctgctggt accggccggt ctctcgaagg cgctcggtgc gatcaccgag gttgcatccg
    14641 ggtttttcga atacttcagc cgcagcgact ggccgaagaa ctggccggcc ctgcgttcgt
    14701 gtttcccggt cagggccaat ctcggccaat ggcttcgctg ggaagccgca ctggaggaaa
    14761 tccgggcgta ttacagcgtg ccaaatcaat ttcgcctgat ggcactgacg atgttcggca
    14821 agggcgtgca gcgaatcatc gcatcgtcgc cgtcacttcg gctgttgccg ctgcagcaac
    14881 ggcgtgaaga cggcagcgtc gacgatgagg aactggcgcg gacgacgatc tttccgtttc
    14941 tggtcaggcg tggcggcgga tttttctcgt tgggcgattg ccggacgatc catcgcgcgc
    15001 ttggccggga tctgcgaaat ggcgtccctg ccaacaagca ccggcggccg gagatcgcgg
    15061 cgaagctttg cctcgtcggt caacccgtcg cagtgggtca tttcgcggcg cttcgcatct
    15121 gtgccagcgc gcgcctcgtc accgagacct ggtcatccga ccagcaagcc gcgcacgaaa
    15181 atgtgcggcg cgaactcggc cgcgtcggca ccatcgtctc aaagatcgag tggctggttg
    15241 cgcatatggg tggtctgaat ttgacggagg cttgccatga agcttgagtg taccgaagag
    15301 gccggtgtcg ttcgctatcc ttgcgcggac cgcatcggat tcgccagact cacgacgatg
    15361 gcgtttcacg ggactgatct tcgccccctg cgggacgaat tgatctcgaa ggtcgccggc
    15421 ggaacagccg acgcgggcga ggggctcgat ctgtcgttga tagcccagct gctcggcgac
    15481 aagcagacgg gattggcgat tcaggcggaa gtgctcgcct ttcaccgcct gttccggtcg
    15541 ccctgttcgg cggaaaagcc gaaactgcgg gtgcttgcgc tcgcggccgc gatcgacatg
    15601 ggcggcaaca cgccgatcga atttttgctg gaggactccg gcatcgagct gctgacgctg
    15661 tatgttattg ccggggccga gttgccggta cccttgcccg atcatgacat cgcgatcgtg
    15721 atcgcgtccg actccgagga atgccgcgac gcgctccgca tcatcgatcg ggcagcgacg
    15781 cgctggccgc gcccgatcct gaatctgccg cgccttgtcg gcaatctgga tcgcgacaag
    15841 ctgcacgggc tgctcaaggg gatcgaaggc cttgatatcc cggcaaccgc atccgtgagc
    15901 agggctcaat tatccgatgt ggctcggtcg aagctcttgt ttgcggacat tgcggccgag
    15961 ccgcggtttc cgatcatcgt ccggccgcgt ggttcgcatg ccggcgtcgg gctcgccaag
    16021 ctcgatgatc acgccgccat ggacaggtat ctcgcagaac gaccggagtc ggaattcttc
    16081 gtgtcgcgct tcgtcgatta tgccaacgat gatggcttgt ttcgcaaata tcgcatcgtc
    16141 ttcgtcgatg gacggccgta tgcctgccac atggcg
//
LOCUS       DATNOI010000346         4343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1432332,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000346 DATNOI010000000
VERSION     DATNOI010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1432332"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VN968_13760"
     CDS             complement(<1..546)
                     /locus_tag="VN968_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXL30338.1"
                     /translation="MIALVRIALSRPYTFVVLALLLLIIGPLAALRTPTDIFPDIRIP
                     VIGVVWQYTGLPPDQMAGRITTPFQRALTTTVNDIEHIVANSYNGFGIVKIFFQPNVD
                     IRTANAQVTAISQTLLKQMPPGATPPLILNYSASTVPIIQVALSGEGLTEQNLADIGI
                     NQLRTPLVTVPGAAIPYPFGGK"
     gene            complement(741..2834)
                     /locus_tag="VN968_13765"
     CDS             complement(741..2834)
                     /locus_tag="VN968_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL30339.1"
                     /translation="MNLRIRTILPLIISALALLLVVSAGLTAYDAIGRRQETQAFLKI
                     NQISQLLLLSAGQWAIERGMTNASLKSADVLSPERRAEIVKLRASADQAFRDAVQRLG
                     AIPAMKAGENDITDAERIFREFEAFRAKVDENLARPGAERTPEIVQGFFPALTNLILV
                     AGNRLRLTLEMLNSPPSVTLAQLVGLRHLTAQMAENAGRERGFLGGIIGARSKLATEG
                     ARTVAGFRGQVELAWETIVPVRRRADTPAKIVAAIDGVESEYFGKYGEIRDAVFAAGD
                     TGEYKVTGSEYVARATTAINSILHLANEIGNASNDEANDEASRSTSSLTLNAAMLFAG
                     IVLAVISFWVAFRRILGPLNAMTGAMTRLAGGDKTIDIPGMNNQDEIGEMGATVQVFK
                     DNMIETDRLRAEQSDVEQRQLQQRKAEMHKLAAAFEGAVSEIVENVSAAATELEASAS
                     TLTSTAERAQQLATAVAAASEEASTNVQSVASASEEMTSSVNEISRQVQESSRVANEA
                     VDQAQKTNDRVGELSKAAARIGDVVELINTIAGQTNLLALNATIEAARAGEAGRGFAV
                     VASEVKALAEQTARATGEISQQISGIQAATQDSVAAIKEIGDTIGRMSGISSTIAAAV
                     EEQGAATQEISRNIQHAAQGTSQVSANVTDVQRGASKTGSASSLVHSAAQSLSGESNR
                     LKLEVGKFLNSIRAA"
     gene            complement(2877..>4343)
                     /locus_tag="VN968_13770"
     CDS             complement(2877..>4343)
                     /locus_tag="VN968_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL30340.1"
                     /translation="QKRAMTRVAALRYGNNDYFWINDMQPRMVMHPMKPELNGGDLSA
                     NKDPNGKALFIDFVNVVRKDGAGFVPYEWPKPGFERPQPKLSYVVGFAPWNWVIGTGV
                     YIDDLDAQTWASTQRSLIAAGLVLLLALAVSMFVARGITRPLQRMTGAMKDLAGGKLD
                     VEVPGIGRRDEVGEMADAVEVFKNNASARRELEAEQKETEGRAVARRKADMDRLANEF
                     EGAVGQIVETVSSASSELEASAGTLTSTAERAQELTTMVAAASEEASTNVQSVASATE
                     EMASSVNEISRQVQESARMAGEAVDQARNTNDRVGELSKAAARIGDVVELINTIAGQT
                     NLLALNATIEAARAGESGRGFAVVASEVKALAEQTAKATGEIGQQITGIQAATQESVN
                     AIKEISGTIEKLSEISSAIAAAVEEQGAATQEISRNVQQASHGTQQVSSHITDVQRGA
                     SETGSASSLVLSAAQSLSGESNRLKLEVGKFLNSVRAA"
BASE COUNT          730 a         1375 c         1359 g          879 t
ORIGIN      
        1 cttgccgccg aacggatagg gaatcgcggc gccgggcacc gtgaccagcg gcgtgcgcag
       61 ctggttgatg ccgatgtccg cgagattctg ctcggtgagg ccctcgccgg acagcgcgac
      121 ctggatgatc ggcaccgtgg acgcgctgta attgaggatc agcggcggcg tcgcgcccgg
      181 cggcatctgc ttgagcagcg tctgcgaaat cgcggtgacc tgggcgttgg cggtgcggat
      241 atcgacattc ggctgaaaga agatcttgac gataccgaag ccattatagg agttcgcgac
      301 gatgtgctcg atatcgttga ccgtcgtggt cagcgcccgc tgaaacggcg tcgtgatgcg
      361 cccggccatc tggtccggcg gcaggccggt atactgccag accaccccga tcacgggaat
      421 ccggatgtcc ggaaaaatgt cggttggcgt gcgcagcgcg gcgagcggtc cgatgatcag
      481 gagaagcagc gccagcacga cgaaggtata cggccggctc agtgcgatac ggaccagtgc
      541 aatcatgaac caggcattcc tcggtccccg cgaaagcgcc gggattagcg caacacggat
      601 caatgcattc gcatcgattt acccgaagcg ggccaaaaaa ccaacgcccg aatgcgatca
      661 cttccctggc atcccggtcg cggcggccgc tgccggcgac gaatccggca gtcaagaccg
      721 gccttgcaag accggccttg tcaggccgcc ctgatcgagt tgaggaactt gccgacctcg
      781 agcttgaggc gattgctctc accggacagc gattgcgccg cggagtgaac cagcgaggag
      841 gccgatccgg tcttgctggc gccgcgctgc acgtcggtga cgttggccga gacttgcgac
      901 gtgccttggg cggcatgctg gatgttgcgg gatatttcct gcgtcgccgc gccctgctct
      961 tccaccgccg cggcaatggt cgaggagatc cccgacatcc ggccgatggt atcgccgatc
     1021 tccttgatcg cagcgaccga gtcctgcgtg gcggcctgga ttccgctgat ctgctgactg
     1081 atctcgccgg tcgcccttgc ggtctgctcg gcgagcgcct tcacctcaga cgccaccacc
     1141 gcgaagcccc ggccggcttc gccagcccgt gccgcctcga tggtcgcgtt cagggccagc
     1201 aaattggttt gacccgcaat ggtgttgatc agttcgacca cgtcgccgat gcgggcggcg
     1261 gccttcgaca attcaccgac gcggtcgttg gttttctgcg cctgatccac cgcttcgttg
     1321 gcgacgcggg acgattcctg aacctggcgg ctgatctcgt tgaccgacga ggtcatctcc
     1381 tcgctcgcgg aagccaccga ctgcacattg gtagaggctt cttcggacgc cgccgcaacc
     1441 gcagttgcga gctgttgtgc ccgttcggca gtcgacgtca gcgtactggc ggaagcttca
     1501 agctccgtcg cagccgcgga cacgttttcg acgatctcgc tcaccgcgcc ttcgaatgca
     1561 gcggcaagct tgtgcatttc cgccttgcgc tgctgcaact gccgttgctc gacgtccgat
     1621 tgctcggcgc gcagccgatc ggtttcgatc atgttgtcct tgaatacctg aacggtggca
     1681 cccatttcgc cgatctcgtc ctggttgttc attcccggga tatctatcgt cttgtcgccg
     1741 ccggctagtc ttgtcatcgc acccgtcatg gcgttcaacg gtccgagaat acggcggaac
     1801 gcaacccaga agctgatgac tgcgagtact atgccggcaa acagcatcgc cgcgttgagc
     1861 gtgaggctcg atgtgcttct cgacgcctcg tcattcgcct catcatttga cgcattgcct
     1921 atctcattag cgagatggag gatcgaattg atagcggtcg ttgcgcgggc gacgtactcg
     1981 ctgccggtga ccttatattc tccggtgtcg ccggctgcga aaactgcgtc gcggatttcg
     2041 ccatacttgc caaaatactc gctctcgacg ccgtcaatcg cagcgacgat ctttgccggg
     2101 gtatcggcgc gccggcggac tggcacgatc gtttcccagg caagctcgac ctggccgcgg
     2161 aaaccggcga cggtccgcgc cccctcggtg gcgagcttgc tgcgtgcgcc tatgatgccg
     2221 ccgaggaacc cacgttccct gccggcattc tctgccattt gcgcggtcag gtgacgaaga
     2281 ccgacgagtt gcgcaagcgt aaccgaaggc gggctattca gcatctcaag cgttaatcgc
     2341 agcctgtttc cggccaccaa aatcagattg gtcagggcgg ggaaaaagcc ctgcacgatt
     2401 tcaggtgtcc gttcggcacc gggcctggcg aggttttcat cgaccttggc gcggaacgcc
     2461 tcgaactcgc gaaaaatacg ttcggcgtcg gtgatgtcgt tctcgccggc tttcatcgcc
     2521 ggaatggcgc ccaggcgctg caccgcgtcg cggaaggcct ggtcggccga agctcgcaac
     2581 tttacaatct cggcacgacg ctccggcgac agcacatcgg ccgatttgag cgacgcgttt
     2641 gtcatcccgc gctcaattgc ccattgacct gcgctaagca acagcaattg cgagatttga
     2701 ttgatcttga ggaaggcttg ggtctcttgc cgcctaccga ttgcgtcata ggcggtgagc
     2761 ccggcggaaa cgacgagcag caaggcgagg gcggaaatga tcaacggcag aatcgtgcgg
     2821 attctaagat tcacttggcc gtttccttct gctgcggttc gtgcgttcga atatcgtcag
     2881 gccgccctga ccgagttgag gaacttgccg acttcgagct tgaggcggtt gctttcaccg
     2941 gacagcgatt gcgccgccga cagaaccagc gacgaggccg agccggtctc gctggcgccg
     3001 cgctgcacgt cggtgatgtg ggaactgacc tgctgggtgc cgtgggaagc ctgctggacg
     3061 ttgcgggaaa tttcctgggt cgcggcaccc tgctcttcca ccgccgctgc gatggccgat
     3121 gatatttcgg acaatttttc gatggtgccg ctgatttcct tgatggcgtt gaccgattcc
     3181 tgggttgccg cctggatgcc ggtgatctgc tggccgatct cgccggtggc tttcgcggtt
     3241 tgctccgcca gcgccttgac ctcggaagcc acgacggcaa agccacggcc agattcgcca
     3301 gcgcgtgccg cctcgatggt ggcattcagc gccagcagat tggtctgtcc cgcgatggtg
     3361 ttgatcagtt cgacgacatc gccgatgcgg gctgccgcct tcgacaactc gccgacacga
     3421 tcgttggtgt tgcgggcctg atccacggcc tcgccggcca tccgggccga ttcctgcacc
     3481 tgacggctga tctcgttgac ggatgaggcc atctcctcgg tcgcggaggc taccgactgc
     3541 acgttggtgg aggcttcctc ggacgccgcg gcaaccatcg tcgtgagttc ctgtgcgcgt
     3601 tccgccgtgg aggtgagcgt accggcggag gcttcaagtt cgctcgaagc cgacgacacg
     3661 gtttcgacga tctggccgac cgcgccctcg aattcgttcg cgagcctgtc catgtccgcc
     3721 ttgcgacggg cgacggcgcg gccctccgtc tccttctgct cggcctccag ttcccggcgg
     3781 gcggaggcat tgttcttgaa cacctcgacc gcgtcggcca tctcgccgac ctcgtcgcgc
     3841 cggccgatgc cgggcacctc gacgtcgagc ttgccgccag cgaggtcttt catcgcgccg
     3901 gtcatgcgct gcagcggcct ggtgatgccc cgcgcgacga acatcgacac ggcgagcgcg
     3961 agcagcagga ccagccccgc tgcgatcagg gatcgctggg tcgaagccca ggtctgcgca
     4021 tcgaggtcgt cgatatagac gccggtgccg ataacccaat tccaaggagc aaagccgacg
     4081 acgtaggaca gtttcggctg cggccgctcg aaacccggct tgggccattc gtaggggacg
     4141 aagcctgccc cgtccttcct gacgacgttg acgaagtcga tgaacaaggc cttcccgttc
     4201 ggatccttgt tggcggagag atcgccaccg ttcagctccg gcttcatcgg atgcatcacc
     4261 atgcgcggct gcatgtcgtt gatccagaaa taatcgttgt tgccatatcg gagcgcagcg
     4321 acccgtgtca tcgcccgctt ctg
//
LOCUS       DATNOI010000347         4942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_752384,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000347 DATNOI010000000
VERSION     DATNOI010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752384"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..906
                     /locus_tag="VN968_13775"
     CDS             106..906
                     /locus_tag="VN968_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXL30341.1"
                     /translation="MYETTYHCPSSVDEAAALFAKGAEAKYLAGGHTLIPVMKQRLAA
                     PSDVIDLGRIKELVGIDVSADAVTIKAATTHYDVVTSGPAQKAIPALVHLASLIGDPA
                     VRYRGTIGGSIANNDPAADYPAAVLALGATVTTNKRSISADEFFKGLFSTALADGEII
                     TQVTFPIPAKAGYSKFPHPASRFALTGVFVVKTKSGDVRVAATGASQNGVMRVSAIEA
                     ALKANWSASALDSVTISANGLMSDIQGSSDYRANLIKVMAQRAVTAAG"
     gene            1141..2457
                     /locus_tag="VN968_13780"
     CDS             1141..2457
                     /locus_tag="VN968_13780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXL30342.1"
                     /translation="MKFGIFYEHQLPRPWNEGDEHRLFQEALDQVELADKLGIDYAWE
                     VEHHFLEEYSHSSAPEIFLAACSQRTRNIRLGHGICLMPPKYNHPARVAERIATLDLV
                     SNGRVEFGTGESASLMELGGFNVGFDPVEKRRMWHEGVEQCTNMMVMDPYPGFKGEFF
                     EMPCRNVVPKPVQKPHPPLWVACSNRETIKMAARHGIGALTFAFVDPREARQWVDDYY
                     RIFKEECVPIGHAVNPNVAMVTGFSVHSDEAESRRRGEDGLRFFRYGLAHHYIFGEHK
                     PGRTDIWANFEKARHALPAAGADHGIGTPDQLRTHLRHFEESGVDQTVFIQQGGRNRH
                     SDICEALELFAKDVMPEFRAREMTREKHKQQELAPYIEKAMARKRAMKPMRDEDIPVY
                     LALGRKVAEEGTGTERQKQNARLWADAAKVTLSDPARHGSKTSATS"
     gene            2489..3601
                     /locus_tag="VN968_13785"
     CDS             2489..3601
                     /locus_tag="VN968_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXL30343.1"
                     /translation="MLDQSAPEFSTRASGPLAGFRIVEFAGIGPGPFACMMLADMGAE
                     VVTLDRVGARKNLKSVAGRGRKVVELDLKDKAGITQVLDLLANADALIEGFRPGVMER
                     LGLGPDVVQARNPRLVYGRMTGWGQQGPLAQAAGHDINYISVTGALAAIGPADRPVPP
                     LNLVGDFGGGALYLVVGVLAALLEARKSGKGQVVDAAMCDGAASLMSMFFDMAAAGRW
                     TDQRESNFLDGGAHFYGVYECSCGRFVSIGSIEPQFYALLREHAGLTDADFDKQMDRN
                     AWPELKQKLIDVFKSKTRDDWCEIMEGTDVCFAPILTMNEAPQYPHNAAREIFVTRHG
                     VTQPAPAPRFSRTPSAIRDAAKADIASLTSEWKGGR"
     gene            complement(3724..4842)
                     /gene="hppD"
                     /locus_tag="VN968_13790"
     CDS             complement(3724..4842)
                     /gene="hppD"
                     /locus_tag="VN968_13790"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HXL30344.1"
                     /translation="MGPFPHDAPAATISADNPMGTDGFEFVEYAHPKPEALHALFKLM
                     GYVPVARHRTKKMTVYRQGDINYLVNEEPGTHGFAFVAAHGPCAPSMAFRVVDAKQAY
                     DRALSLGAEPADILPEQKTLDVPAIKGIGGSVLYFVDRYGAKGSAYDSEFEWLGAKNP
                     RPEGAGLYYLDHLTHNVHRGRMDVWTGFYQKLFNFRQIRFFDIEGRASGLFSRALTSP
                     DGKIRIPINEDAGQSGLIEEYLSVYRGEGIQHIACGARDIYRTVETLRRDGLPFMPSP
                     PETYFEKIDARLPKHGEGVARLKRDGILIDGEGVVEGGHTKVLLQIFSANAIGPIFFE
                     FIQRKGDDGFGEGNFKALFESIEEDQIRRGVLTLKGAA"
BASE COUNT          977 a         1533 c         1533 g          899 t
ORIGIN      
        1 cgtcatcaac gccatcaccg atgcgatcgg caacaacaag cttgagatgc cggcaacgcc
       61 cgaccgtgtc tggcacgcca ttcacggttg agaggaaaac gagccatgta cgagacaaca
      121 taccattgtc cctcctcggt cgacgaagcc gcggcccttt ttgcaaaggg cgcggaggcg
      181 aaatatctcg ccggcggtca cacgttgatc ccggtaatga aacagcgcct tgccgcgccg
      241 tccgacgtga tcgatctcgg acggatcaag gaactggtcg gcatcgacgt ttccgccgac
      301 gccgtgacca tcaaggccgc gacgacacac tacgatgtgg ttacgagcgg acctgcgcaa
      361 aaggcaattc ccgcgctcgt ccatctcgcc tcgctgatcg gcgacccggc cgtgcgctat
      421 cgcggcacga tcggcggctc gatcgccaac aacgatccgg cagcggacta tccggcggcg
      481 gtgctcgcac tcggcgcgac ggtgacgacc aacaagcgtt cgatatcagc ggatgaattc
      541 ttcaagggcc tattctcaac cgcgctggca gacggcgaga tcatcacgca ggtcacgttc
      601 ccgattccgg ccaaggcggg gtattccaag tttccccatc cggcgtcgcg cttcgcgctg
      661 accggcgtgt tcgtggtcaa gaccaaatca ggcgacgtcc gcgtcgccgc caccggcgca
      721 tcgcagaatg gagtcatgcg ggtatccgcc atcgaggcgg cgctgaaggc caactggtcg
      781 gcttcggcgc tggacagcgt cacgatttcc gcgaacgggt tgatgagcga cattcaaggc
      841 tcgtcggact atcgcgccaa tctgatcaag gtgatggcgc agcgcgcggt cactgcggcc
      901 ggctgatcgc gcatctcgcc tcgaaccatg caggcggcgc gcagcgatgc gcgccgctct
      961 gcttttgggc atattctgat caaaaggcgc ttgccacgcg gcgcgcaagg cgtgacaatt
     1021 tagctaatca gctaattaaa acgctggtgc gtgatccgga agaaacctgc cccgcacttt
     1081 tgtgcgggtg ggcacccgtt ttccgaaaag atcaggcaca accaatgggg tggagcgtcc
     1141 atgaaattcg gcattttcta cgaacaccag cttccgcggc cgtggaacga gggcgacgag
     1201 cacaggctgt ttcaggaggc gctcgatcag gtcgagctcg ccgacaagct cgggatcgat
     1261 tacgcctggg aagtcgagca ccattttctc gaggaatatt cgcactcctc tgccccggaa
     1321 atattcctcg cggcctgctc gcagcgcacc aggaacatcc gtctcggcca cggcatctgc
     1381 ctgatgccgc cgaaatacaa tcacccggcg cgggtcgccg agcgcatcgc aacgcttgat
     1441 ctcgtctcca acggccgcgt cgaattcggc accggggaga gtgcctcctt gatggagctc
     1501 ggcggcttca atgtcggctt cgatccggtc gagaagcggc ggatgtggca tgaaggcgtc
     1561 gagcaatgca ccaacatgat ggtgatggac ccctaccccg gcttcaaggg cgagtttttc
     1621 gagatgccgt gccgcaacgt ggtgccaaaa ccggtgcaga agccgcaccc gccgctctgg
     1681 gtcgcctgct ccaatcgcga gaccatcaag atggcggcgc gtcacggcat cggcgcgctg
     1741 acctttgcgt ttgtcgatcc acgtgaggcc cggcaatggg tcgatgacta ctaccggatt
     1801 ttcaaggagg aatgcgtgcc gatcggccac gcggtcaatc ccaacgtcgc gatggtgacg
     1861 ggcttttcgg tacactcgga tgaggcggaa tcgcgccgcc gcggcgagga cggtttgcgt
     1921 ttcttccgct atggcctcgc gcatcattat atcttcggcg agcacaaacc gggccgcacc
     1981 gacatctggg ccaacttcga aaaggcgcgg catgcattgc cggcggccgg cgccgaccac
     2041 ggcatcggca cgccggatca gttgcgcacc catctgcggc acttcgagga atcaggcgtc
     2101 gaccagaccg tgttcatcca gcagggcggg cgcaaccggc acagcgatat ctgcgaagcg
     2161 ctcgagctgt tcgcgaagga cgtgatgccg gaatttcggg cgcgcgagat gacgcgggaa
     2221 aagcacaagc agcaagagct cgcgccctat atcgaaaagg ctatggcgcg caaacgggcc
     2281 atgaagccga tgcgtgacga agacattccg gtctatctcg cgctggggcg aaaagtcgcc
     2341 gaagaaggca ccggtaccga acgccaaaaa caaaatgcca ggctgtgggc cgacgccgcc
     2401 aaggttacct taagcgatcc cgcgcgccac ggcagcaaga cctccgcgac atcatagtcc
     2461 ttcctgtctc atcaacggaa aaaacaacgt gctcgatcaa tcagccccag agttctcgac
     2521 ccgcgcgtcc ggcccgcttg cgggtttccg tatcgtcgag ttcgccggca tcggacccgg
     2581 tccctttgcc tgcatgatgc tggcggacat gggcgcggaa gtggtcacgc tcgatcgcgt
     2641 cggcgccagg aagaacctga aatcggtggc ggggcgcgga cgaaaggtcg tcgaactcga
     2701 cctgaaggac aaggccggga ttacgcaggt gctcgacctc ctggccaatg ccgatgcgct
     2761 gatcgaaggc tttcgtcccg gcgtgatgga gcgtctgggg cttgggcccg acgttgtgca
     2821 ggcgcgcaac ccgcgcctcg tctacggccg catgaccgga tggggccagc aaggtccgct
     2881 ggcgcaggcc gccggccacg acatcaatta tatctcggtc accggcgcgc ttgccgcgat
     2941 cggacctgcg gaccgaccgg tgccaccgct caatctagtc ggcgatttcg gcggcggcgc
     3001 actttatctg gtggtcggcg tgctcgccgc cctgctggag gcacgaaagt ccggcaaggg
     3061 acaggtggtg gacgccgcga tgtgcgacgg cgcggcatcg ctgatgtcga tgttcttcga
     3121 catggcggcc gcgggccgat ggaccgatca gcgcgagagt aatttcctcg acggcggcgc
     3181 gcatttctac ggcgtctatg aatgctcctg cggccggttc gtttcgatcg gctcgatcga
     3241 accgcaattt tatgcgttgc tgcgagaaca cgcagggctc acggacgctg atttcgacaa
     3301 gcagatggat cgcaatgcct ggcccgagct gaagcagaag ctgatcgatg ttttcaagtc
     3361 caagacccgc gacgactggt gtgaaatcat ggagggcacc gacgtctgct tcgcgccgat
     3421 cctgaccatg aacgaggcgc cccagtatcc gcacaatgcg gcacgcgaga tctttgttac
     3481 ccgccacggc gtcacccaac cggcgccggc accgcgcttt tcacgcacgc cgtcggcgat
     3541 cagggatgcg gccaaggcgg atattgcaag cctgacgagt gagtggaagg gcgggcggta
     3601 accctctctt cgtcattccg gggcgcgcgc agcgcgagcc cggaatccat aaccacgaca
     3661 gggagtatgg attccgggcc tgcgccaagt ggcgcatccc ggaatgacga aggcgaggta
     3721 aatctacgcc gcgcccttca gcgtcagcac gccgcggcgg atctggtctt cctcgatcga
     3781 ttcgaacagc gctttgaagt tgccttcgcc gaaaccatcg tcgcccttgc gctggatgaa
     3841 ctcgaagaag atcggcccga tggcgttggc ggaaaagatc tgcagcagca ccttggtatg
     3901 gccgccctcg accacgccct cgccgtcgat caggatgccg tcacgcttga ggcgcgcgac
     3961 gccctcgccg tgcttgggca gccgcgcgtc gatcttttcg aaataggttt cgggtggcga
     4021 cggcatgaac ggcaggccgt ccctgcgcag cgtttcgaca gtccgataga tatccctggc
     4081 gccgcaggcg atgtgctgga tgccctcgcc gcgatagacg ctgagatatt cctcgatcag
     4141 accggattgg ccggcgtcct cgttgatcgg aatccggatc ttgccgtcgg ggctggtcag
     4201 cgcccgcgag aacaggccgg atgcgcggcc ctcgatatcg aagaatcgaa tctgccggaa
     4261 attgaacagt ttttgataga agccggtcca gacatccatg cggccgcgat gaacgttgtg
     4321 ggtaaggtga tccaggtaat acagcccggc cccctccggt cgcggatttt ttgcgcccag
     4381 ccattcgaac tcggaatcat aggccgagcc tttggcaccg tagcgatcga caaaatacag
     4441 cacgctgccg ccgataccct tgatcgcggg aacatcgagg gtcttctgct caggcaaaat
     4501 gtcagccggt tccgcgccga gcgacagcgc gcgatcatag gcctgcttcg catcgacgac
     4561 gcgaaacgcc atcgacggcg cgcatggccc gtgcgcggcg acaaaggcaa agccgtgggt
     4621 gccgggctct tcgttgacga gataattgat gtcgccctgg cgatagaccg tcatcttctt
     4681 cgtcctgtgg cgcgcgaccg gcacataacc catcagcttg aacagcgcgt gcagcgcttc
     4741 gggtttggga tgcgcgtatt cgacgaactc aaagccatcg gtccccatgg gattatcggc
     4801 gctaattgtc gccgccggcg cgtcgtgcgg aaatggaccc atggctgaac tccctgatct
     4861 tgcttcaggc agtttccgct atatcggccg caaagtgcat gcaaagcagc ccgggatgtg
     4921 ccataatcat gcacattctg tg
//
LOCUS       DATNOI010000348         9586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1080955,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000348 DATNOI010000000
VERSION     DATNOI010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9586
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080955"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1959
                     /locus_tag="VN968_13795"
     CDS             <1..1959
                     /locus_tag="VN968_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HXL30345.1"
                     /translation="PPSPGRQRTRPPKQKKSKMERAIAVCLEQLGFGAAPPAGRTNSA
                     PAAKRIGSAAAAGRSNTASPTGRINGVPPSGRINSGSPAGRINGAPPSGRRGERPVEP
                     NFKPPPRRRVVTLDNAIPMDLLKPTPELRGSDNRRPPTPIDQPRLAGPQSTPMEPMDK
                     PPLALPRPVGNVEPKTTEPKTTLGQLIEKGRVGVSTGVAFLVNRDGLAHNDNAPGDTL
                     ALRTRRSYERELRVGLRVLLIGLVVGGGWMTLVPLAGAVVVPGNLVVQSNVKTIQHPT
                     GGVVAEIPVHNGMRVVVGDLLLRLDPTQAQASLQVVSKQLDESRARIARLIAERDGLP
                     QPEVPAELAGRLEESAVKDLLVSEETQFKARASARYSQKDLLQSRVAQLGEQIGGLDA
                     QVASKAKQLELIAGELTGVQDLYDKRLVPITRLTTLQREAARIEGERGQLISAIAETK
                     AKVGEAQLQIVRIDQDFRTEVVKELGEVQGKEGELVQHGVAARDMLDRIELRAPTSGI
                     VHQLAAHTVGGVIKAGDAIMEVVPDSDDLQVEARLQPNDIDQVRVGQKALIRFSAFNQ
                     RTTPQIVGLVSFVSADVSHDQTASAANVSFFTVRMTLPEDELRRLAGLQLVSGMPAEV
                     FMQTGSRTMMSYLMKPITEQLQRAFVER"
     gene            complement(2069..2683)
                     /locus_tag="VN968_13800"
     CDS             complement(2069..2683)
                     /locus_tag="VN968_13800"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXL30346.1"
                     /translation="MTHLANRIMEHYERHATAWDSDRQNSAWNDKVWHDRFIDRLTAG
                     AKVLDLGCGSGRPVARHMAERGLRVTGVDSSPTMISLCRSRLPDHAWIVADMRDLSLA
                     RRFDGILAWDSFFHLDHDDQRRMFAVFAAHASAGAWLMFNAGPRHGEAIGSYRGDPLY
                     HASLSPGEYETLIECCGFEVIEHAADDERAGGRTAWLCRSRDGA"
     gene            complement(2948..3460)
                     /locus_tag="VN968_13805"
     CDS             complement(2948..3460)
                     /locus_tag="VN968_13805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL30347.1"
                     /translation="MAEVTLPRPLSETDDRGRFDCGRESLNHWFLRHAWANHAAGISR
                     TNVICDAATAEIIGYVTLSAAQVERAFLPKSQQRNKPDPVPATLLGQLAIHKDHQGKG
                     HARSLLLFALRAALRASRDIGSFGVITHPLDDSVRAFYGRWGFQDLPFDPHRAMIVRM
                     VDLERSGIEQ"
     gene            complement(3448..3711)
                     /locus_tag="VN968_13810"
     CDS             complement(3448..3711)
                     /locus_tag="VN968_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HXL30348.1"
                     /translation="MTTLVLSVRVNEEERALLEAASALARTSLSDFIRRKALDAAEAD
                     ILERRIVTIPAKDWEGFEAWANRPPEEIAGLKDLAGKTPTWQK"
     gene            complement(3977..4951)
                     /locus_tag="VN968_13815"
     CDS             complement(3977..4951)
                     /locus_tag="VN968_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL30349.1"
                     /translation="MISVVVVAALALLALATQIGVLVLQRIYPAQGKTIEVAGASLNV
                     VDIGPRDAAGPPIVMIHGASSNLRAMRQSLGDRLAENHRVILIDRPGHGWSTRARRDD
                     STPEIQGRMIDEALERLGVGGVILVVHSWSGALGARIALDYPRRVAGLVMLAPVAYPW
                     PGGVGWYNKAITTPVIGPLLAYTITLPLGLFLAEPAARGAFLPQIVPENFVKNTATPL
                     LLRPREFLANAQDLVTLKAAVKEQAPRYANIKAPTVVIAGDVDRTVSTNIHSRPFAAA
                     VPNAKLIVLPGVGHMVQNAAPELVISEIDAMIDGIARNAAAQSETDRF"
     gene            complement(5167..5874)
                     /locus_tag="VN968_13820"
     CDS             complement(5167..5874)
                     /locus_tag="VN968_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HXL30350.1"
                     /translation="MLLIGPVAVSAVKYLLGDRSVDWQTADRSSAGLLPPPVGYPDAL
                     IRVFAARTVRWRGIFAVHSWIVIKELNAPRYTRYDYTAWGAPIRINGFAADGRWFGGM
                     PETIAAADGEAAERMIPRIRAAVEGYKLRAYGDYRAWPGPNSNTFVTALLAAVPELET
                     TLPPTAIGKDFPYDGDVLGLTPSKTGVRISLGGYLGLTIGWIEGIEINLLGAVFGIDL
                     RRPAVKLPGLGRVGFGY"
     gene            6079..6252
                     /locus_tag="VN968_13825"
     CDS             6079..6252
                     /locus_tag="VN968_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30351.1"
                     /translation="MLSKSNEAAIDQIVASCNGDIRGALKALLLVNEHLEAELQQVYA
                     AVAHGVRGDSVLH"
     gene            complement(6265..6490)
                     /locus_tag="VN968_13830"
                     /pseudo
     CDS             complement(6265..6490)
                     /locus_tag="VN968_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453420.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6447..6596
                     /locus_tag="VN968_13835"
     CDS             6447..6596
                     /locus_tag="VN968_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30352.1"
                     /translation="MIANIITPANNNDFNVSLPRFGQAGKFATAKTVPAHHAVSGIVV
                     NSRAI"
     gene            complement(6613..7266)
                     /locus_tag="VN968_13840"
     CDS             complement(6613..7266)
                     /locus_tag="VN968_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL30353.1"
                     /translation="MALRFPAARDCLAAILFTAVIAAAPVSAFAQDPGGTPLKPQKKL
                     PEAPSKLPKVGADRTRGLDFLFGALKAAPDEASAKHVEARIWALWMQTPSDTAALLMM
                     RARAAMDAQQIDVALKLLDAVVKLRPDYVEGWNRRATLYYLKNDYTHSLEDIEQVLVR
                     EPRHFGALAGLGMIMQDIGDDKRALDAFRKALAVNPHLEKVPELVKTLSEKVEGRDI"
     gene            complement(7372..7497)
                     /gene="ykgO"
                     /locus_tag="VN968_13845"
     CDS             complement(7372..7497)
                     /gene="ykgO"
                     /locus_tag="VN968_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HXL30354.1"
                     /translation="MKVRNSLKSLRGRHRNNRLVRRKGRVYVINKVQRRFKARQG"
     gene            7910..8815
                     /locus_tag="VN968_13850"
     CDS             7910..8815
                     /locus_tag="VN968_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HXL30355.1"
                     /translation="MLPAGVVPFQLNWPYILGIGAYHIAALLAFMPGYFSWSGLVLAM
                     LGTHLCGLFGINLCYHRLLTHRGLKCPKWLEHAMVIIAMSCFQETPARWVAIHRLHHQ
                     FADRRPDPHSPLASFFWAHIGWILVNQPELSRLGIYDRYARDILRDRFYVALERNHCL
                     LWINLGQLPLFFGAGFAVAWLSGQPPAAAAQTGLSIMMFGVFVRTVLVWHITWAVNSV
                     THLWGYRNYETDEDSRNNIMVGLIANGEGWHNNHHADPRSARHGHRWWEIDNTYLTIR
                     FLMWLGLASDVVTPTVRPKNHQPAA"
     regulatory      8890..8992
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            9068..>9586
                     /locus_tag="VN968_13855"
     CDS             9068..>9586
                     /locus_tag="VN968_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30356.1"
                     /translation="MKRLLSFAAAFAIGLSTITAASAEPKKEFNIAWTIYVGWMPWPY
                     AAETGIVKKWADKYGITINVTQINDYVESINQYTAGKFDGVTVTNMDALTIPAAGGVD
                     TTAIIMGDYSNGNDGIVLKKGKTLADIKGQKINLVELSVSHYLLARGLSTVKLQEKDI
                     KTVNTSDADIVAA"
BASE COUNT         1890 a         2999 c         2924 g         1773 t
ORIGIN      
        1 ccgccttcgc ccgggcgaca gcggacacgc ccgccgaagc aaaagaaaag caagatggag
       61 cgggccatcg cggtctgtct cgagcaattg ggattcggcg cggcccctcc ggccgggcgc
      121 accaattccg ctcccgcggc caaacgcatc ggttccgctg ccgcggctgg acgcagcaat
      181 accgcttcgc cgaccgggcg catcaatggc gtgcccccat ccgggcgcat caattccggt
      241 tccccggccg ggcgcatcaa tggcgccccc ccatccgggc gcagaggcga acgtccggtc
      301 gaaccgaatt tcaagccgcc gccgcgccgt cgcgtcgtca cgctcgacaa cgccatcccg
      361 atggatctgt tgaagcccac acccgagctt cgcggatcgg acaatagacg tccgccgacg
      421 ccgatcgatc agccccgatt ggccggcccg cagtcaacac cgatggaacc gatggataag
      481 cctccattgg ctcttccgcg tccggtcggc aacgtcgaac ccaaaacgac tgaacccaaa
      541 acgactcttg ggcagttgat cgagaaaggc cgcgttggcg ttagcacagg cgttgccttc
      601 ctggtcaacc gcgacggcct ggcccacaac gataacgcgc cgggtgatac gctggcgctg
      661 cggacccgac gctcgtacga gcgggagttg agggtcggcc ttcgggtgct gcttatcggc
      721 ctcgttgtgg gcggtggctg gatgacgctg gtgccgctgg caggcgcggt cgtggtgccc
      781 ggtaatctgg tggttcaatc caacgtcaag accatccagc atccgaccgg cggggtcgtg
      841 gcggagattc cggtccataa tggcatgcgc gtcgtcgtcg gcgatttgct gctgcgtctc
      901 gacccgaccc aggcgcaagc cagtcttcag gtggtgagca agcaacttga cgaatcccgg
      961 gcgcggatcg cccgactgat tgccgagcgc gacggcctgc cgcagcccga ggttccggct
     1021 gaactggcgg ggcggttgga ggaaagcgcg gtcaaggatc ttctggtgtc ggaggagacg
     1081 cagttcaagg ctcgcgcgag cgcgcgttac agccagaagg atcttctgca aagccgcgtc
     1141 gcacaactcg gcgagcagat cggaggcctc gatgcccagg tcgcgtccaa ggcgaaacag
     1201 ctggaactga tcgcagggga actcacggga gtccaggatc tgtatgacaa gcggctggtg
     1261 ccgatcacac ggctgacgac gttgcagcgc gaggcggcgc ggatcgaagg cgaacgcggt
     1321 caattgatct ccgcgatcgc cgagaccaag gcaaaagttg gcgaagccca gctccagatc
     1381 gtacggatcg atcaggattt tcgtaccgag gtcgtcaagg aactcggcga agtgcaaggc
     1441 aaggaaggcg aactcgttca gcacggcgtc gccgcccgcg atatgctcga tcggatcgaa
     1501 ctccgcgcgc cgacttcggg gattgtccat cagcttgcag cgcatacggt tggcggcgtc
     1561 atcaaagccg gcgacgccat catggaagtg gtgccggatt cggacgactt gcaggtcgag
     1621 gcgcggctgc aacccaacga tatcgaccag gtgcgggtgg gacagaaggc gctgatccgc
     1681 ttctcggcct tcaaccaacg cacgacaccg cagatcgtcg gcctggtgtc ttttgtgtcg
     1741 gccgatgtca gccatgatca aacggcaagt gcggccaatg tgtcattctt cacggtcagg
     1801 atgacgctgc cggaggacga acttcgccgg ttggccggac tgcagcttgt ctcgggcatg
     1861 cccgcggaag tcttcatgca gaccggcagc cggaccatga tgagctacct catgaaaccg
     1921 attaccgaac agttgcaacg cgcattcgtc gagcgatgag ccggagcgtc gagtgcgagg
     1981 aaacccggga tcgcgcggag cgagtgaaat cgcgatcaaa gcaggtcgcg ctgagctgaa
     2041 ggagcgaagc gggagacggc gccacaattc acgcgccgtc ccgcgatctg cagagccacg
     2101 ccgtgcgccc gccggcgcgc tcatcgtcag cggcgtgttc gatcacctcg aacccgcaac
     2161 actcgatcag ggtctcatac tcgcccggtg agagactggc gtgatacagg gggtcacccc
     2221 gatagctgcc gatcgcctct ccgtgccgcg gtcccgcgtt gaacatcagc caggcaccag
     2281 cggatgcgtg cgccgcgaac accgcgaaca tgcggcgctg gtcatcatgg tcgaggtgaa
     2341 agaaactgtc ccaggccagg atgccgtcga agcgccgagc tagcgacaag tcccgcatgt
     2401 cggccacgat ccacgcatga tcgggcagac ggctgcggca aagcgatatc atcgtcggcg
     2461 atgagtcgac gccggtcacg cgcaagcccc gctcggccat gtgtcgtgca acaggccgtc
     2521 ccgagccgca gccgaggtcc agaacctttg cccctgccgt gagcctgtcg ataaagcggt
     2581 catgccagac cttatcgttc caggcgctgt tctggcggtc gctgtcccac gccgtggcgt
     2641 gtcgctcgta gtgctccatg atgcggtttg cgaggtgcgt catgctgccg atatgggcgc
     2701 gcgccacgat cgatcaagac gggcactacg cgttcgctat tgccctatga cacagtgcgt
     2761 gtcgatggga tgcggttcgc gagcccgcga acgggccgcc atgtgccctt cgccttcgcc
     2821 gccaccactc ttgccgcatt ggcagttcag tggacgacag aaccccggcg ggccgatcga
     2881 gagaacctct gaactcttct cttgcgatcc gcggaacact gcgggcttct tcgtggtcgt
     2941 ggcgggttta ttgctctatg ccgcttcgct cgagatcaac catgcgcacg atcattgcgc
     3001 ggtgagggtc gaagggcaga tcctggaacc cccatcggcc atagaacgcg cgcacagaat
     3061 cgtcgagcgg gtgtgtgata acgccaaagc tgccgatatc tcgggaggct ctgagcgccg
     3121 cacgaagcgc gaacagaagg agcgaacggg cgtggccctt tccttgatga tccttgtgga
     3181 tcgcaagctg gccgagcagt gtcgccggca ctgggtcggg tttgttgcgc tgctgtgact
     3241 tgggcaagaa tgcccgctcg acctgcgcgg cgctcaaggt tacgtaaccg atgatctcgg
     3301 ccgtggcagc gtcgcagatg acattggtgc gcgagatgcc ggcggcgtgg ttggcccaag
     3361 cgtgacgcag gaaccaatgg ttcagcgact cgcggccgca atcaaatcgg ccgcgatcgt
     3421 ctgtttcaga gagcggacgc ggtagcgtta cttctgccac gtgggcgtct tgcccgcgag
     3481 atccttcagc ccggcaatct cctccggcgg acgattcgcc caagcctcga acccttccca
     3541 gtccttggcc gggatggtga cgatccgccg ctcaaggatg tcggcctcgg ccgcgtcgag
     3601 cgctttgcgc cggataaaat cgctgaggct ggtgcgcgcc agcgcggacg cggcctccag
     3661 cagggcgcgc tcttcctcgt tgacgcgtac gctcagcacc aaagtggtca tggcacgtgt
     3721 tcctgtggac tccgcaagtg tatgacaata gcatacatcg gataccgccg gcaatcccgg
     3781 cgatggtgtt tcggatgtgc cgggaggcgc agatagcgaa atcccgtacc gccgcgagtc
     3841 ccacgcggcg ccgcgacaag cggttcacga ggcgctatcg ggacaaagca gcgaacgatt
     3901 gcgctgcaac gggacgttgc ggggcgcgac atcgtgctct gcgtgcagtg gcgcgcctcc
     3961 tccgattcgc aatctctcaa aaccgatccg tctcggattg agcagccgcg ttgcgcgcaa
     4021 tcccgtcgat catggcgtcg atctcggaga tcacaagctc gggcgcggcg ttctgcacca
     4081 tgtggccgac acccggcaat acgatcagtt tcgcattcgg caccgcggcc gcgaacggtc
     4141 gggaatggat gttggtcgac acggtcctgt cgacgtcgcc cgcgatcacc accgtcggcg
     4201 ccttgatgtt ggcgtaacgt ggcgcctgct ctttaaccgc cgccttcagc gtcaccaggt
     4261 cctgggcatt ggcgaggaat tcgcgcggac gcaacaataa tggcgtcgcg gtgttcttga
     4321 cgaagttttc tggcacaatc tgcggcagaa atgcgccgcg tgcggctggc tctgccaaaa
     4381 acaggccgag cgggagcgtg atcgtatagg ccagcagcgg gccgatcaca ggagtagtga
     4441 ttgccttgtt gtaccatccg acgccgcccg gccacggata ggcgaccggc gccagcatca
     4501 ccagccccgc gacgcgccgc gggtaatcga gcgcgatgcg cgcgccgagc gcaccgctcc
     4561 aggaatggac caccaggatc accccgccga cgcccagtct ttccagcgct tcatcgatca
     4621 ttctgccctg gatttcgggc gtcgaatcgt cgcgccgcgc gcgcgtgctc cagccgtgcc
     4681 cgggacgatc gatcaggatc acccgatggt tttcggcaag cctgtcgccg agcgattgcc
     4741 gcatcgctcg cagattggag ctcgcgccat ggatcatcac gatgggggga ccggcggcat
     4801 cgcgcggccc gatatcgacg acgttgaggc ttgcgccggc gacctcgatg gtcttgccct
     4861 gcgcgggata aatgcgctgc aggacaagca cgccgatctg cgtggccagc gccaggagcg
     4921 ccagcgccgc cacgacaacg actgatatca tttggaatgt ccggatgagt tttggcacca
     4981 atgagttacg gtttcgaaag ctgatggttt cgcggctgaa ttcagacgag agcgggatga
     5041 cttttcttcg aaaagtcatc ccgctctatc tttttgtttg agcatgatcg ccgcgcaaac
     5101 gtttcgcgtt tgtcgcgagg gaaaaccggt acccactttt ccggatcatg ctctctagcc
     5161 cgcgcatcaa taaccaaaac ccacgcgtcc cagtcccggc aatttgacgg ccggccgccg
     5221 cagatcgatc ccgaacaccg cgccgagcaa gttgatctcg atgccttcga tccagccgat
     5281 cgtcaggccg agatagccgc cgagggagat gcggacaccc gttttcgaag gcgtcagtcc
     5341 gagcacgtcg ccatcataag gaaagtcctt gccgatcgcg gtcggcggca gcgtggtttc
     5401 cagttccgga actgccgcca gtagggccgt gacaaacgta ttggaattcg gtcccggcca
     5461 ggcgcgatag tcgccgtatg cccgcaattt gtagccttcg accgcggcac ggattctggg
     5521 aatcatgcgc tcggcggctt cgccgtcggc ggcggcgatc gtttccggca tgccgccgaa
     5581 ccaccgccca tcggcggcaa acccgttgat ccgaatcggc gcgccccagg ccgtataatc
     5641 atagcgtgta tagcgcggcg cgttcaactc cttgataacg atccagctat gcaccgcgaa
     5701 aattccgcgc cagcgaaccg tccgcgccgc aaagacccga atcaaggcat cgggatatcc
     5761 aaccggaggc ggcagcagtc cggcgctgga gcgatccgcc gtctgccaat cgacagagcg
     5821 atcgcccagc aaatatttca cagccgatac agcgacaggg ccgatcagca acatcagaaa
     5881 aacaatcaca gattttctca acttgcctga acacgccgcc tttttatcgc cgtcatcggc
     5941 agcattcata tacgcgtgat cagcagtttt gccatgtagc cgagcgtgct cgatcgaagc
     6001 tgtcgtctat ccacattatc cacaggaacg ctggttaggc gcgaaagatt atcccacggc
     6061 aaagttgaag gatccgagat gctctccaaa tcgaatgaag ccgccatcga ccagatcgtg
     6121 gcaagctgca acggcgatat ccgcggcgcg ctaaaagccc tgctgctggt caacgagcat
     6181 ctggaggccg aacttcagca ggtctatgcg gcggtggctc atggcgtgcg cggcgatagt
     6241 gtcctgcact gatcgccggt cggttcactc gtcaggcggc ttggggcagg cgcggatggt
     6301 cgagcgcgcg agctcggcat cgttgaggga tttatagggt ccgtcttcga accagacatc
     6361 gccgatcaca accggattac cggtgacgat ctcgcatgtg ttggtcgcgc ggttgccgac
     6421 cacccaattc cgcgagacaa accgtcgtga ttgcaaacat catcacgccg gccaacaaca
     6481 atgatttcaa cgtgtcgctc cctcgcttcg gtcaagcggg aaagttcgcg acagcgaaaa
     6541 ccgttccggc gcaccatgca gtgtcgggaa ttgtggttaa ttcgcgggcg atctgaccga
     6601 tcaacagatc gatcagatat cgcggccctc gaccttctcg ctcagggtct tgaccagctc
     6661 cggcactttt tcgaggtgcg ggttgacggc caatgccttc ctgaaggcat cgagcgcgcg
     6721 cttgtcgtcg ccgatatcct gcatgatcat gccaagtccc gccagcgcac caaagtggcg
     6781 cggttcgcgg accagcacct gttcgatatc ctcgagcgaa tgggtatagt cgttcttcag
     6841 ataatatagc gtcgcgcgcc gattccatcc ctcgacataa tcgggacgca gcttgacgac
     6901 cgcatcgagc agcttcaacg cgacatcgat ctgctgggcg tccatcgcgg ccctggcgcg
     6961 catcatcaag agcgcggccg tatcgctcgg ggtctgcatc catagcgccc agatccgggc
     7021 ctcgacgtgc ttggcactcg cctcatcggg cgccgctttc agtgcgccaa acagaaagtc
     7081 cagtccgcgc gtgcgatcgg cgccgacctt cggcagcttg ctcggcgcct ccggcagttt
     7141 tttctgcggc ttcagcgggg tcccccccgg atcctgtgca aacgccgaaa ccggcgccgc
     7201 cgcaatcacc gcggtgaaca ggattgcggc gagacagtcg cgcgcagctg ggaatctcaa
     7261 tgccatcgcc gaagtctaga cgcagaaaaa cgcgctgcaa agcagcaaga cgtcaaagac
     7321 ctgtgaagaa aaattcgccc gatggacccg aaacggccct ttaggctcgc ttcaaccctg
     7381 gcgcgccttg aaccggcgct gaaccttgtt gatcacgtaa acccggccct tgcggcggac
     7441 caggcggttg ttgcgatgcc gaccgcgcaa cgatttcagc gagttacgga ccttcatggg
     7501 acaatcctga tgctttgaaa ggccgtgttc ggcacttatc gtttgcgagc ccgaaagtgg
     7561 caaaatgggc ttcatcccgc cgacggctgc gccgcccggg acgcgcggtt tctaagccat
     7621 ggagtggccg aatgtcaatc caaacgggga gaccacccgg cgtgcggcta accgccacaa
     7681 tggaaccaat ccttcgccga tgtacaaaat agacgaattc agtcaaatgt ttgcagcgat
     7741 acgattcgtc cgcatgtcgt gaatcaaaac tgtttcagcc gatagctgcg tccggcgcag
     7801 aaattgggat cccgaccccg ctatgacgac agtccatcaa ccggccacaa agcccagagt
     7861 gccggccgct ggggaatgtg ccgctcccgt tgcagcgaat gagcggctga tgctgcccgc
     7921 aggcgtggtg ccgtttcagc tcaactggcc ctacattctg gggatcggcg cctatcacat
     7981 cgcggcattg ctggccttca tgcccggcta tttcagttgg agcggcctcg tcctggccat
     8041 gctcggcacc catctgtgcg ggctgttcgg catcaacctt tgctatcacc ggctcctgac
     8101 ccatcgcggc ctgaaatgtc ccaaatggct ggagcacgcg atggtgatca tcgcgatgtc
     8161 ctgcttccag gaaacccctg cccgctgggt cgcgatacac cgcctgcatc atcaattcgc
     8221 cgaccggcgg ccggatccgc acagcccgct tgcaagtttc ttctgggccc acatcgggtg
     8281 gattctggtc aaccagccgg aactgtcgcg gctcggaatt tacgaccgct acgccaggga
     8341 catcctgcgc gatcgctttt acgtcgcgct cgaacgcaat cattgtctgc tctggatcaa
     8401 tctcggccag ctgccgctgt tcttcggcgc cggattcgcc gtggcatggc tatcgggtca
     8461 gccgccggca gccgcggcgc agaccggact cagtatcatg atgttcggcg tgttcgtgcg
     8521 cacggtcctg gtatggcata ttacgtgggc cgttaattcg gtgacgcatc tgtggggtta
     8581 ccggaactac gagaccgacg aggacagccg caacaacatc atggtcggtc tcatcgccaa
     8641 tggcgagggc tggcataaca accatcatgc cgatccgcgc tcggcgcgcc acggtcatcg
     8701 ctggtgggaa attgacaaca cctatttgac cattcgcttt ttgatgtggc tcgggctggc
     8761 gagcgacgtg gtgacgccaa ccgtgcgccc aaaaaaccat cagcctgccg cttagacggg
     8821 ctgaaaacga cgcatgcgca cccgattccg ggcatttccg ttggcacatc gattgcttca
     8881 ttgatggaag agtgtggcgg ctagggttcc ggcttgatca agcgtctggt ccgagagctg
     8941 ccgcccggga gcgtcacttc cgggtgcacg gcgggataaa agcccgggag gcctcgatcg
     9001 ccaggattgg catcgcaggg tcttgccaat cctcgaattc gcaacgcatc agcgaggatt
     9061 gagattcatg aaacgccttt tgtcattcgc cgccgctttc gcgatcggcc ttagcaccat
     9121 caccgcggct tcggccgagc cgaagaagga attcaatatc gcctggacga tctatgtcgg
     9181 ctggatgccg tggccctacg ccgccgagac cggcatcgtg aagaaatggg ccgacaaata
     9241 cggcatcacc atcaatgtca cgcagatcaa cgactacgtc gaatcgatca accagtacac
     9301 cgcgggaaaa tttgacggcg tgaccgtcac aaatatggac gccctgacga tcccggccgc
     9361 cggcggcgtc gacacgacag ccatcatcat gggcgattat tccaacggca acgacggcat
     9421 cgtgctgaag aagggcaaga ccctggccga catcaagggc cagaagatca atctcgtcga
     9481 actgtcggtg tcgcactatc tgctggcgcg cggcctgtcg accgtgaaac tgcaggaaaa
     9541 ggatatcaag accgtcaaca cttccgatgc ggatatcgta gcggcc
//
LOCUS       DATNOI010000349        24486 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_927586,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000349 DATNOI010000000
VERSION     DATNOI010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 24486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24486
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927586"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VN968_13860"
     CDS             <1..301
                     /locus_tag="VN968_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXL30357.1"
                     /translation="CFIPKGDTVMTIQASANRDEDVFEDGENYNGLREPNPHQAFGNG
                     PHHCAGAHLSRRTVGAILLPMLFERFPDMTLPDPASVRWHGFGFRGPLNLPVLLQ"
     gene            complement(373..1014)
                     /locus_tag="VN968_13865"
     CDS             complement(373..1014)
                     /locus_tag="VN968_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30358.1"
                     /translation="MVHDHHRGDIPLVSVDKRSSDSKSSDAATRSEAAQAWTAIKDTN
                     DPAVLNAFIRRFDNTFYSDLARNRLQQLNRTEIATTTPPTGNPAQQNRASAPPGPPAD
                     DPQTASNGIVGQWSWRSHCGIFGSYQGNVQFTQSASKKLAGVMSDDSGDWTLFDGRVG
                     DGVISFRRKVKGGATQHWTATLDRPKSGKYLLKGTVSDSRFSIGSCTWIATRG"
     gene            1462..1872
                     /locus_tag="VN968_13870"
     CDS             1462..1872
                     /locus_tag="VN968_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30359.1"
                     /translation="MQKVNRRSAVALGLAAASAAMVKPAASQTTDIVGKDSTPWPGVV
                     VRVYGETPAIIPGFKNVQLRDIIMQPGSKTMGPPMMNAMVCHITEGELRIEQEGKTFT
                     AKKNFVWTCNKDTKEQAYNDGNAVGIMRITDLMA"
     gene            2292..3605
                     /gene="soxC"
                     /locus_tag="VN968_13875"
     CDS             2292..3605
                     /gene="soxC"
                     /locus_tag="VN968_13875"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HXL30360.1"
                     /translation="MPKGIRHPSGDAVAGNGLIDRRALLGQGVAIAGALSTGVGASLT
                     SAAAEPLKDDPWSLEPGSSIKPYELPSRFEKNVARTLNNPDGLPFVQTARTPHHMLNG
                     MITPNGLHFVIAYGGPPDIDPDKHRLVIHGLVKRPLVFTLEALARYPMVSRISFLECG
                     GNSAPLFSPQPLQGNVQALHGLASCAEWTGVKLSTLLEETGIDPKAKWFIAEGADAPH
                     LTRSVPVAKALDDAMIALYQNGERLTPSNGYPVRLLLPGYEGNMNVKYLRRIKLVAEP
                     AMGFWETKTYTQLLPNGEAYQFYFLQEVKSFITQPSPGMTLKQPGLYQISGVAYSGTG
                     RIARVMVSADGGQSWAEAALQEPVLAKAFTRFRMAWHWTGGAAMLQSRAWDEAGNFQP
                     TRAEFVAQRGELKNVAPIGAFPNQHFNAVTSWAVDAKGQISHAYT"
     gene            3610..4128
                     /locus_tag="VN968_13880"
     CDS             3610..4128
                     /locus_tag="VN968_13880"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXL30361.1"
                     /translation="MVDSASLVFALSISVAFADGPNLGTPIDPADVAAWDISVMPDGA
                     GLPAGSGTPAQGERIYAEKCVACHDNDGKNKFGFSPLFGGGKITDISAGMKTIANFWP
                     YSTTLFDFIRRGMPWQQPKSLTDDEVYALTAFILAKNKLIGETDTMNPETLPKVRMPN
                     RGGFIIRFPDKI"
     gene            4213..4662
                     /locus_tag="VN968_13885"
     CDS             4213..4662
                     /locus_tag="VN968_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL30362.1"
                     /translation="MARITVNRKSHDIDVDPNTPLLWVLREQVGLTGTKYGCGIAQCG
                     ACTVHIDGAAVRSCSLPVSEAEGKQITTIEGLAQNGVLHPVQKAWLEHDVPQCGYCQT
                     GMIMAAVALLMEKPKPSDADIDSTVTNICRCGTFQQVRAAIHAATNA"
     gene            4676..6853
                     /locus_tag="VN968_13890"
     CDS             4676..6853
                     /locus_tag="VN968_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL30363.1"
                     /translation="MNHVVRMNRRSFVIGTAAVGGGLALGLELQIGSGEADAAASEPE
                     VNAWVVIRPDETVVIRVAKSEMGQGTITGLAQLVAEELECDWSKVTFEYPTPGQNLAR
                     KQVWGTMFTAGSRGIRESQELVRKGGAVARQMLLQAAANGWNVPVSECSAVNGVIAHK
                     SSGRTTTFGKVAESAGKLEPPKDVQLKDPKDWKIAGRPLKRLDTIDKLTGQLIYGIDV
                     KLPGMLNAAIKDCPVFGGKLKSFDAKKIMGMKGVQKVVRVGESAVAVVADTWWQAKTA
                     LDALPIKWDEGENANISSASIAEWLKAGLDAEQTFVGNQNGDVKAALASAAKKVEAVY
                     SYPYQNHATMEPMNTTALYTADKCEVWCPTQNGDRALAAVIEASGLSAPKCEVHKLPL
                     GGGFGRRTFTDYIHQAVAIAKEVPGAPVKLLWSREEDMAHGRYHPITQAKMVGGFDAN
                     GNLTALHMRISGQSILASVRPESLQNGRDPATFQGLNPSGPEAAIGYTIPNLLIDHAM
                     RNPPVPPGFWRGVNINHNAIYLECFMDELAHSIGQDPLEFRRRLMAQHPKHLAVLNAV
                     AEKIGWAKSPPQEIHRGIAQMMAFGSYVAAATEISVDNGNKIKIHRIVAATDCGTAVN
                     PAQIERQIAGSFVYGLSALFYQECTLNGGRIEQQNFDTYDSMRIAQMPKVESIIMPSD
                     GFWGGVGEPTICVAGPAVLNAFFNATGQRIRSFPLKNHNIQFA"
     gene            complement(7384..7614)
                     /locus_tag="VN968_13895"
     CDS             complement(7384..7614)
                     /locus_tag="VN968_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HXL30364.1"
                     /translation="MSPDRLIYMANQIGKFFHSQGHDKAVPGIAEHIRKFWDPRMRAA
                     IFAHLDAGGAGLDPDVRDALTTLKDADAKLKA"
     gene            complement(7604..8434)
                     /gene="fdhD"
                     /locus_tag="VN968_13900"
     CDS             complement(7604..8434)
                     /gene="fdhD"
                     /locus_tag="VN968_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HXL30365.1"
                     /translation="MHDPVQSVSRKVWRGDGASEGARAIPEEIAIALTYNGGTYAVMM
                     GTPKNLRDFAVGFSLSEGVVQSADEIEQLDVVDLDDGIELRMWLAQTKADRLAARRRH
                     IAGPTGCGLCGIDSIAEALRPAAIVRHGRAFSPQLIMTAMQSVASLQKINIETRAVHA
                     AAFWTQARGIIALREDVGRHNALDKLAGALALEKVATDDGMVLLTSRVSVEMVQKTAT
                     MGAALMVAVSAPTALAVRMADAAGITLAAIARADGFEVFTHPQRIAAGVAAQGAFHVA
                     "
     gene            complement(8438..11317)
                     /gene="fdhF"
                     /locus_tag="VN968_13905"
     CDS             complement(8438..11317)
                     /gene="fdhF"
                     /locus_tag="VN968_13905"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007822296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HXL30366.1"
                     /translation="MSLVHEIDFGTPRFNSEAMVTLTIDGQTVTVPEGTSIMRAAMEA
                     GTQIPKLCATDMVDAFGSCRMCLIEIEGRAGTPASCTTPAMAGLVVHTQTERLKKLRK
                     GVMELYISDHPLDCLTCAANGDCELQDMAGAVGLRDVRYGYQGENHVFAKSDGAANPA
                     WMPKDESNPYFTYDPSKCIVCSRCVRACEEVQGTFALTISGRGFDSRVSPGMSESFLG
                     SECVSCGACVQACPTATLTEKSVIEIGQPEHSVVTTCAYCGVGCAFKAEMRGEEVVRM
                     VPYKDGKANRGHSCVKGRFAWGYTNHRERILHPMIRENISDPWREVSWDEAFNFAAAK
                     FKGIQAKYGRDAVGGITSSRCTNEEVFVVQKLIRAGFGNNNVDTCARVCHSPTGYGLS
                     STFGTSAGTQDFDSVEHTDVVMIIGANPTDGHPVFASRLKKRLRAGAKLIVVDPRRTD
                     IVRSAHVEAQHHLPLLPGTNVAVLTAIAHVIVAEGLVDEAFVRERCDWSEFEDWAAFV
                     VLPKNSPEATAIATGVSPEELRAAARLFATGGNGAIYYGLGVTEHSQGSTTVIAIANL
                     AMATGNIGRPGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHISGDAVRETFESMWNVK
                     LNKEPGLRIPNMFDAAIEGTFMGLYVQGEDILQSDPNTKHVVAALSAMECVIVHDLFL
                     NETANYAHVFFPGSTFLEKNGTFTNAERRIQRVRKVMSPRNGLEDWEVTLGFARALGY
                     EMHYAHPSEIMDEIAALTPTFAGVSFAKLDELGSVQWPCNEKAPLGTPVMHIDGFVRG
                     KGKFMVTEYIATDERTGPRFPLLLTTGRILSQYNVGAQTRRTANVVWHQEDRLEMHPH
                     DAEQRGVRDGDWIRLTSRAGETTLRALITERVAPGVVYTTFHHPDTQANVITTEYSDW
                     ATNCPEYKVTAVQISPSNGPSEWQKAYDEQARHSRRIAPVVEAAE"
     gene            complement(11330..12886)
                     /locus_tag="VN968_13910"
     CDS             complement(11330..12886)
                     /locus_tag="VN968_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HXL30367.1"
                     /translation="MSLRIFVSRDAGAVAVGADEVALVLGLAAGKRGIDIEIVRTGSR
                     GVYWLEPMIEVATSQGRIAFGPVTEFDAGSVLDAMISGGAHPLWLGVADEIPWLKRQT
                     RLTFARCGVIDPRSLEDYRAHAGYKGLERALTLTSDAILADVTISGLRGRGGAGFPTG
                     IKWKTVAQTGADRKYVVCNADEGDSGTFADRMIMEGDPFVIIEGMTIAGITVGATKGY
                     IYIRSEYPHAVAAMNAAIAAATRAGYLGARIGGSSHSFDLEVRVGAGAYVCGEETSLL
                     ESLEGRRGIVRAKPPLPAHKGLFGKPTVINNVLSFAAIPFILAGGAKAYADFGMGRSR
                     GTMPIQLAGNVKRGGLFEVAFGITLGELVDDIGGGTDTGRPVRAVQVGGPLGAYFPRA
                     LFDTPFDYEAFAARDGLIGHGGIVVFDDSVDMSKQARFAMEFCAIESCGKCTPCRIGS
                     TRGVETIDRIRAGDRVAENIAVVEDLCNTMKFGSLCALGGFTPYPVMSALKHFREDFG
                     AAPPRLQAAE"
     gene            complement(12883..13359)
                     /locus_tag="VN968_13915"
     CDS             complement(12883..13359)
                     /locus_tag="VN968_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HXL30368.1"
                     /translation="MMPVYEPWNAARGAEIIAEHNRLEGATLVILHALQEAFGYVPEP
                     AVPMIASALNLSRAEVHGVFTFYHDFRRGPAGKHVLKLCRAEACQAAGGDALAARAEA
                     RLGITLGNTTADERVTLEPIYCLGLCATAPSAMLDGRVVGRLNEARIDALIAEAQR"
     gene            13596..14489
                     /locus_tag="VN968_13920"
     CDS             13596..14489
                     /locus_tag="VN968_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL30369.1"
                     /translation="MIDKLELLLALAKERHFGRAAEACGVTQPTMSTSLKQLEEILGV
                     MLVQRGSRFQGFTPEGERTLDWARRIVGDARAMRQEINGLKDKLSGEIRIAAIPTVLG
                     MVASLTTPFRARYPDVLFRIVSCTSADVLGLLENLEVDAGLTYIENEPIGKVRTIPLY
                     DESYRLLTAPDAMFGDRDTVTWKEVGQVPLCLLTPDMQNRRIIDRALRSVGAEATPTL
                     TSNSLLVLYTHVKTGRWASVMPAKLAETLGLADTVRSIPIVDPVVTYSVGLVIPQRDP
                     MTPLIAALVHTVREVAPTLES"
     gene            complement(14491..16815)
                     /locus_tag="VN968_13925"
     CDS             complement(14491..16815)
                     /locus_tag="VN968_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL30370.1"
                     /translation="MARAHAATGCVQSDSIKGLAQSIAKPAYHRLLIAEPALRRAVPT
                     LIIAFLITICLGAFVQVVDQNRQQRASMKRDLAALADLLAERLDRIASAREDRTATVE
                     RLQGLVPGLIPSWGIAAGRHVIVTGADQRILARVPVETSLGDPDRILDAISTAQLLAA
                     PAHQGAITDMILPSGGVALAVQHVVKSLPGHVIIVQEKVDSLWRSDAALSVTLSATTG
                     FVVLILGFAFHWQSTRAREGDLINDAVRGRIDTALNRGRCGLWDWDLSRGRIFWSQSM
                     FTLLGLDTRSDLLTFGEVNALVKSDDIDLFAIADQLISSKINHIDQTFRMQHTDGHWI
                     WLRVRCELSQGEADAGLHLIGIAVDITEQKSLAEKTVEADLRLRDAIETIPEAFVLWD
                     AEDRLVLCNSHFQRLHKLPDSAVTPGTSYETVIEVGSMPEVRTRLHEAGTQPPGARTF
                     EAQLDNGSWLHISERRTKDGGYVSVGTDITRIKEHEQKLVDNDLRLRATVIDLKRSQT
                     ALEQQALELADLAEKYSEEKNRAEEANQTKSKFLANMSHELRTPLNAIIGFSEIMESG
                     MFGTLGSEKYQEYCHDILTSGHYLLEVINDILDMSKIEAGRMKLDMESLDLSKTLAES
                     LRVVSGRADDKNLVLDADIEGPISVVADRRAIKQIIVNLLSNAVKFTPEGGKVIVRSR
                     VLSDSILLMIADTGIGIAPHSLARLGRPFEQVESQLTKTYHGSGLGLAIARSLANLHG
                     GSMRLRSKLGAGTVVCVSLPRDAGKARAKMSVAA"
     gene            complement(17022..18404)
                     /locus_tag="VN968_13930"
     CDS             complement(17022..18404)
                     /locus_tag="VN968_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXL30371.1"
                     /translation="MIHPDHSVKSRVAAISIFASSGMAAAKFVVGIAIGSLALISEAL
                     HSSVDVVATVVTWLVVRVSDRPADHEHHYGHGKFESLSALGVIAMLYVLAGGILVESY
                     SRLREGAPPPTISAIPFIVLLIDIAVNFWRARALHRTARDTKSQALAADALHFASDVL
                     GSIAVMVGLALSGLGYAWGDAAAAIAVAIVISSLGLRLARSTVETLLDRAPEGASEEA
                     IAAIRAVPGVVDVERLRMRMVGSTHFIDAIVQVPRTYPIDRVEEIKRKAQEAVSKVLD
                     DADLTFTAVPVARNNESIRERIMVIARNSGLAIHHVTVHNLGGKLTVSIDLEVDAEMA
                     LNQAHEIAHELERNIRDEFGEDVEVDTHIEPLEPELPLGADATPDRVDTIKTALSRFA
                     ADGAIHDIHSVRVRNTDAGEIVNFHCRAAPSMSVIKVHENVDAIERALRRAFPTVKRV
                     ISHAEPPDAQ"
     gene            complement(18569..19360)
                     /gene="fetB"
                     /locus_tag="VN968_13935"
     CDS             complement(18569..19360)
                     /gene="fetB"
                     /locus_tag="VN968_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HXL30372.1"
                     /translation="MNPILLSPFDISIAASLIILDAALSILLKLRLHRQIAIAAARMV
                     VQLVAIGYVLRFIFTLNNPAATLLLILVMVLVAAREVAARPERRFKDYSNLVIGFVSV
                     GFATFVTSILALTTAIRPQPWFDPHYAIPLAGIILGNVLNSASLSLDSFLGSVRRERQ
                     AIEARLSLGDSYREAIAPLVREAIRRGLLPIINQMSAAGIVTLPGIMTGQILAGLDPL
                     EAVKYQILLMFLLSGGSGLSALAASYLAAWRLTDDRQRLRLDRLQ"
     gene            complement(19357..19962)
                     /locus_tag="VN968_13940"
     CDS             complement(19357..19962)
                     /locus_tag="VN968_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL30373.1"
                     /translation="MATALLVKDLRSAFAGPFELNLGTGACAAITGPSGSGKSLFLRM
                     IADLDPNEGKVWLNGRERGSMPAPQWRRRAAYVSAESGWWADRVIEHFDVSRRSEVAA
                     LAARLGLRADLLDAPVAQLSTGEKQRFSLVRALLPSPPVLLLDEPTGPLDEESVAKVE
                     ALLRERKATGTSILLVTHDPNQAERLGDLRYRMVAGQLQTP"
     gene            complement(20090..20947)
                     /locus_tag="VN968_13945"
     CDS             complement(20090..20947)
                     /locus_tag="VN968_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXL30374.1"
                     /translation="MTPRRPMPSGVLDGGRYFEGPRWHLGRLWFVDCMERTLLSLDAS
                     GACEQHAKFEDDTPCGLGVLPDGKLVVLTMFRKRLMSYADGELSLYADLSGIATGTID
                     DMIVDGLGRAYVGDLGFDLPPPPDRGPVGRIILVMPGGERRVVADGLRFPNGIAVSAD
                     NKRLVVAEMDGECLAEYDIEADGGLNFCRRFGNMKARDGICLDHEGAVWVASFGEDAF
                     IRVNRDGRELQRIELPGRRAIACVLGGPERRTLFCLSAATSPEQLRQRQSSARIDVAE
                     VEIPGAGYP"
     gene            complement(20944..21918)
                     /locus_tag="VN968_13950"
     CDS             complement(20944..21918)
                     /locus_tag="VN968_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXL30375.1"
                     /translation="MKAYVYGANGAEITEVAKPAPKGPQVLVRVRACGLNRADLGMTK
                     GHVHGSAGGAGTVLGMEWAGEIADVGPEAKGVKVGDRVMGSGSAAFAEYTLADHGRLF
                     HIPASANMSYEDAATLPVALATMHNAVVTNGALQPGQSVLIQGASSGVGLMALQIAKL
                     KGAKIVIGSSTDATRRGRLKEFGADLAVDSSDPGWVDQVLEATGGEGVDLIVDQVSGK
                     VASQNLAATRIKGRIVNVGRLGGTHADFNFDLHAARRITYIGVTFRTRSIEEIREIFG
                     EVKKDIWGAVEQRRLQLPIDKVFAFDEIGKAFEHMEANKHLGKIVVTL"
     gene            complement(21955..22950)
                     /locus_tag="VN968_13955"
     CDS             complement(21955..22950)
                     /locus_tag="VN968_13955"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HXL30376.1"
                     /translation="MIKTRFTELVGVEHPIVQGGMQWVGRAELVAAVANAGALGFITA
                     LTQPTPDDLTREIARCRSLTDKPFGVNLTILPSIKPPPYAEYRQAIIESGIKIVETAG
                     NKPQEHVTEFKKHGVVVVHKCTSVRHALSAERMGVDAISIDGFECAGHPGEDDTPGLI
                     LIPVAADKVKIPMIASGGFGDGRGLVAALALGAEGINMGTRFMCTKESPIHQLIKEKI
                     VANDERETELIFRTMRNTSRVARNAISTQVVAMEKEGATFEQVRELVAGARGKMVYAT
                     GDADEGIWSAGQVQGLIHDIPSCAELVSRIMRDAEAIIRSRLEGMMSGARRQAAE"
     gene            complement(23118..23526)
                     /locus_tag="VN968_13960"
                     /pseudo
     CDS             complement(23118..23526)
                     /locus_tag="VN968_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458572.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
     gene            complement(23626..24060)
                     /locus_tag="VN968_13965"
     CDS             complement(23626..24060)
                     /locus_tag="VN968_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXL30377.1"
                     /translation="MQVGDILRKKTARVATVRMNETVAIAAQLMRASNISALVVKDVV
                     RTEGNTAVGMFTERDVVRAIAEHGAAGVNLKVSQLISVGQLVSCSSADTLEHVRHLMN
                     RNHIRHLPVIDDYSLVGVVSIRDISTAFDDQATGAARSASAA"
BASE COUNT         4625 a         7516 c         7671 g         4674 t
ORIGIN      
        1 atgcttcatc ccgaagggcg acaccgtgat gacgatccag gcgtcggcca atcgagacga
       61 ggacgtcttc gaagatggcg agaactacaa cgggctgcgc gagcccaatc ctcaccaggc
      121 ctttggcaac ggcccgcatc actgcgcggg cgcgcacctg tcgcggcgaa ccgttggcgc
      181 catcctgctg cccatgctgt tcgaacgctt ccccgacatg accttgcctg atcccgcgag
      241 cgtgcgctgg cacggattcg gtttccgcgg cccgctcaat ctgccggttc tcctgcaata
      301 gaaccggcgc gcgggagagg cgtttatttt gtctcatccg ctgccgcaga gcgcgggccg
      361 ttgctgaatc gttcagcctc tcgtcgcaat ccaggtgcag cttccgatcg agaatcgcga
      421 gtctgagacc gtgcccttga ggagatattt gccgctcttc ggacggtcca gcgtagccgt
      481 ccagtgctgc gtggcgccgc ctttcacctt ccggcggaag gaaatcacgc cgtcgccgac
      541 gcgcccgtcg aacagcgtcc agtcgccgga atcgtctgac atcaccccgg ccagtttctt
      601 gctcgcggac tgggtgaatt gcacgttgcc ttgatagctt ccaaaaatcc cgcaatgcga
      661 ccgccaggac cattgtccga caataccgtt gcttgccgtc tggggatcat ccgccggcgg
      721 acccggcgga gcgcttgccc tgttttgctg ggcgggattg cccgtcggcg gcgtcgtcgt
      781 agcgatctcg gtcctgttga gctgctgcag tcgatttctg gcgaggtccg aatagaacgt
      841 gttgtcgaag cgcctgatga acgcgttcag cacggccggg tcgttggtgt ctttgatggc
      901 ggtccaggcc tgcgccgctt cgctacgggt tgcagcgtcg gaggacttcg aatcggaaga
      961 ccgcttgtcg acagatacaa gcgggatatc gccccggtga tggtcatgaa ccacaagacg
     1021 acaccatgac ttcgggcttt ctggtccttg gccctgccac ggcttttcgg caggtacgat
     1081 ggcccaacgg caccaagcgc aagcatcgca gtcgtcagcg ccgaaaccga ccgttgcagg
     1141 ttttgttaca tctcattacg gcagcgatcg aaagagatgg cgattatggc agtagcgctg
     1201 tcttgaagtc gcctcgcgac cagtcatgtc agcatagtcg acgagacgcg taggacttat
     1261 cgacattgcg gtaggcaacg cgtgcgcctc ggcatatcgc aacgttccac cacgcgagct
     1321 acgcatcgta ccacatcgct acgcaacgcg cgagccgctg gggatacccg gcgactctct
     1381 cgcgtatatc gcggacggtc accctatcgg gcgataggag caactttgcg gcctcgtggc
     1441 cttagcgtga ggagaaggtc aatgcaaaaa gtaaatcgaa ggtcagcagt ggcactaggt
     1501 ttggcagccg cttcggcggc aatggtgaag ccagcagcat cgcaaaccac ggacatcgtc
     1561 ggcaaggact cgacgccgtg gccgggcgtt gtggtgcgcg tttatggcga gacaccagcg
     1621 atcatccccg gttttaagaa cgtccagttg cgtgacatca tcatgcagcc gggatcgaag
     1681 accatgggtc ctccgatgat gaatgccatg gtctgccata tcaccgaagg ggagcttcgg
     1741 atcgagcagg agggaaagac ttttacggcc aagaagaact ttgtctggac ctgcaacaag
     1801 gacacaaaag agcaggcgta caatgacggg aacgcggtcg gaattatgcg gatcaccgac
     1861 ctaatggcct gattaaggcc cgcgggaggc cgcctcagtt ggcggcctct ttcatatgtt
     1921 ccgcatcttg agcgcgggat tgcggaccgt tctatccgtg accaccatgt tcgcagacaa
     1981 gccgcaccgc ctcaacggac gaactagcgc gcaattgacc cgattcagac attgcggtcg
     2041 gctgattttg gccgaattgc ttgttccgcc caggccaata tgaggtcttt tggatagatg
     2101 tgggatggtg taaacttgtt ttgagctgct cttcgagacg ggacgtgccg acgtctccgt
     2161 tcgaggtgga cgggcagcgg gaatgccgtc ggcgggcatc gcactctcaa taaaaccgac
     2221 agtccttttg tgccggtctc tcaccgagca ctcgccgtgc gcacgggcac gcgacagctg
     2281 aggaggcggt catgcccaaa ggaattcgtc atccttcggg ggatgctgta gctggaaacg
     2341 gtctgattga ccggcgtgca ctgctcgggc agggcgtcgc tatagcaggc gcgctcagca
     2401 cgggcgtcgg ggcctcgctg acaagcgccg cggccgagcc actcaaggat gatccatgga
     2461 gccttgagcc cggttcgagc atcaaaccgt atgagctgcc gtctcggttt gagaagaacg
     2521 tcgcgcgaac gctgaacaac cccgacggcc tgccattcgt tcaaactgcg cgtaccccgc
     2581 atcacatgct aaacggaatg atcacgccga atggcctgca tttcgtgatc gcctatggcg
     2641 gcccgcccga catcgatccg gataaacacc gcctcgtgat ccacggcctc gtcaaacgcc
     2701 ccttggtgtt cacgcttgag gcacttgcgc gctatccgat ggtgtcgcgt atcagcttcc
     2761 tggaatgcgg cggcaacagc gcaccgctgt tttcccccca gccgctgcag ggcaacgttc
     2821 aggcactgca cggcctcgcg tcttgtgctg agtggacggg tgtgaagctc tccaccctgc
     2881 tcgaagaaac cggcattgat ccgaaggcga aatggttcat cgccgaaggc gcggacgcgc
     2941 ctcacctgac acgcagcgtg cccgtggcga aggccttgga cgacgccatg atcgccctct
     3001 atcaaaatgg cgagcgcctc acgccgagta acgggtatcc ggtgcgcctg ctgttgcccg
     3061 gctacgaggg caacatgaac gtcaagtatc tgcggcgcat caagctcgtg gcggaaccag
     3121 ccatgggctt ctgggagacc aagacctaca cgcagcttct cccgaacggc gaggcatacc
     3181 agttttattt cctgcaagaa gtcaaatcgt tcatcacgca gccgtctccc ggaatgaccc
     3241 taaagcagcc cggcctttac cagatttccg gtgtcgcata ttccggcacc ggccgtatcg
     3301 ccagggtgat ggtttcggcc gatggcgggc agagttgggc cgaggcggcg ctgcaggagc
     3361 cggtattggc gaaggctttc acccgtttcc gcatggcttg gcattggacc ggtggcgccg
     3421 caatgcttca aagccgggcc tgggatgaag ctgggaactt ccaacccaca cgcgccgagt
     3481 tcgtagctca gcgcggcgag ttgaagaatg tcgcaccgat cggggcgttt cccaaccagc
     3541 acttcaacgc ggtcactagt tgggcggtcg acgccaaagg gcagatcagc catgcctata
     3601 catagaattt tggtcgatag cgcttcgttg gtgttcgctc tgagcatcag cgtagcgttc
     3661 gccgacgggc ccaatcttgg cacgcctatc gaccccgccg acgtcgcggc ttgggatatc
     3721 agcgttatgc ctgacggcgc cggcctgccg gcaggaagcg gtacgccggc gcaaggcgag
     3781 cggatttacg cggaaaaatg cgtcgcctgc cacgacaacg acggcaagaa caaattcggt
     3841 ttttcaccac tgttcggagg cggcaagatc accgacatat cggccgggat gaagaccatc
     3901 gcgaactttt ggccttattc cacgacgctc ttcgacttca tccgtagagg gatgccgtgg
     3961 cagcagccga agtcactcac cgacgacgag gtttacgctc tgaccgcgtt catcctggcg
     4021 aaaaacaaac ttataggtga gaccgacacg atgaaccccg agacgctgcc caaggtgcgg
     4081 atgccgaacc gtggcggctt catcatccgt ttccccgata aaatttaagt tgaaatgtcg
     4141 gctcatggca cctttgagac acgtcgcccc ccgtcttttc cgagcagata cctttaatca
     4201 gggaggagaa gaatggcccg tattaccgtc aatcgtaaat ctcacgacat cgatgtggat
     4261 cctaataccc ccttgctttg ggtattacgg gagcaggtcg gcctcaccgg gaccaaatac
     4321 ggttgcggaa ttgcgcagtg cggagcctgc accgtgcaca tcgacggcgc tgctgtccgc
     4381 tcctgctccc ttcctgtcag cgaggctgaa gggaagcaaa tcactacgat cgaagggttg
     4441 gctcagaatg gtgttctgca tcccgttcag aaagcctggc ttgagcacga cgtgccacag
     4501 tgtgggtact gccaaaccgg catgatcatg gctgcagtag cactgctgat ggaaaagcca
     4561 aagcctagtg atgcggacat tgacagcacg gtcacaaata tctgccgctg cggcactttc
     4621 caacaggtgc gcgcggcaat tcacgcggcg acgaacgcgt gagagggagg acgccatgaa
     4681 tcacgttgtg cgaatgaacc gccggtcttt tgtcattggc actgctgccg tcggcggagg
     4741 gcttgcgctt gggctcgaac tccagatcgg gtctggcgag gctgatgctg cagcgagcga
     4801 gccggaagtg aatgcgtggg tggtgattcg gcccgatgag actgttgtta tccgcgtcgc
     4861 gaaatccgaa atgggccagg ggacgatcac aggactagct cagctggtcg cggaggaatt
     4921 ggaatgcgat tggtcgaagg tcacgtttga atatcccact ccaggccaga acctcgcccg
     4981 taagcaagtg tggggcacca tgttcaccgc gggcagtcgc ggtatcagag aatcgcaaga
     5041 actcgttcga aaggggggtg ctgtggcccg tcagatgctg ctccaagctg cagctaatgg
     5101 ctggaatgtt ccggtctcag agtgtagcgc ggttaacggc gtgatcgccc acaagtcttc
     5161 gggacgaacg acgactttcg gcaaagttgc ggaatccgcc ggcaaactcg agccacccaa
     5221 agacgtgcag cttaaggatc cgaaggactg gaagatagct ggcaggccgc tgaaacggct
     5281 cgacacgatt gataagctca cgggccaact gatttacggc atcgatgtga aattgccggg
     5341 aatgctgaat gcagcgatca aggattgtcc tgtgtttggg ggcaagttga aaagcttcga
     5401 cgccaaaaag attatgggga tgaagggcgt gcagaaagtc gtccgagttg gcgagtccgc
     5461 ggtcgctgtc gtggccgaca cttggtggca ggccaagacg gcactcgatg ccctcccgat
     5521 taagtgggac gaaggcgaga atgcaaacat ctcgagtgcg tcgattgccg aatggctaaa
     5581 ggccggtctt gatgccgagc agacgttcgt gggcaatcaa aatggtgacg tcaaggccgc
     5641 gctcgccagc gcggccaaga aggtggaggc ggtctactca tacccgtatc agaaccacgc
     5701 gaccatggag ccgatgaaca ccaccgcgct ctacacggcc gataaatgcg aggtctggtg
     5761 ccctactcaa aacggcgacc gtgctctggc ggccgtgatc gaggcatccg gtctatccgc
     5821 tccaaaatgt gaggttcaca agctcccgct tggcggtgga ttcggtcgcc gaaccttcac
     5881 cgactacatt catcaggccg ttgccattgc caaggaagtg ccgggagcac cggtgaagct
     5941 gctctggtcg cgggaagagg acatggcgca cggccgctac catcccatca cccaggcaaa
     6001 gatggtcggt gggtttgatg cgaacgggaa tctaaccgca ttgcatatga ggatctcggg
     6061 gcaatccatc ctcgcctccg tacgtccgga gagcctccag aatggacgcg atccggcgac
     6121 gttccaaggg ctcaacccga gtggaccgga agccgcgatt ggctacacca tcccgaatct
     6181 gctgatcgac catgcgatgc gaaatccacc tgttccaccc ggcttttggc gcggtgtgaa
     6241 tatcaatcac aacgccatct acctcgagtg ctttatggat gaacttgccc actcgattgg
     6301 ccaagatccg ctagagttcc ggcgcaggct gatggcgcag catcccaagc acctggccgt
     6361 gctcaatgcg gtcgcggaaa agatcggttg ggcaaaatct cctccgcagg agattcatcg
     6421 gggaatcgcg cagatgatgg cctttggcag ctacgtggct gccgcaaccg aaatctccgt
     6481 cgataacgga aataagatca agatccaccg gatcgtggcg gcgacggatt gcgggactgc
     6541 agttaacccg gcgcagatcg aacggcagat cgcaggctca ttcgtctacg gtttgtccgc
     6601 cttattttat caagagtgca cgcttaatgg cgggcgcatc gaacaacaga actttgatac
     6661 ttacgactcg atgcgcatcg ctcagatgcc caaggtcgaa tccatcatca tgccaagcga
     6721 cggcttctgg ggtggcgttg gcgagccgac gatctgcgtt gcaggccccg cggttctcaa
     6781 tgccttcttt aatgcgacgg gtcagcggat ccggtcgttt ccgctcaaga atcataacat
     6841 acaatttgca tgagcctgaa atggcggccc gcaagccgct gtgacttggt gaatgttgtt
     6901 catgcacttg attcgcactt tcattcccgc cggagccgtc ttggcgtcga tcaccaaggc
     6961 tgcggtgggt tcctccgagc cgccggtcgg agcggcggcc tgttccggct gcacatgcct
     7021 cctctgctac tcgggtcctg gcgaggcaag ccgatgcttt cggccgagca gatcgaggac
     7081 gtggtagctt ccgtggcggc cttacgtagc tagtctaaat taagaatgat gcacatccat
     7141 gcccacaagg ccgccgcaac gcgccgcaaa tttctggcag cacggttggg ctacccgcgc
     7201 agcgggctat cgcgcagcga tttcgtgcaa ttggcacatc gcatcatttc gccgcgctgc
     7261 agaattcggt cgcaaactgg gatcatagcg gacatcgagc aagccgcccg gcaagctcga
     7321 attatcagtt cgtgctccag ttaaagatag acaagcgacc agcctgattc agcgatgccg
     7381 ggatcaggcc ttgagctttg cgtcggcgtc tttgagcgtc gtgagggcat cccggacgtc
     7441 gggatcaagg ccggcgccgc cggcgtcgag atgcgcgaag atcgcggcgc gcatgcgggg
     7501 atcccagaat ttccggatgt gctcggcgat gcccggcacc gccttgtcgt ggccctggct
     7561 gtgaaagaat ttgccgatct ggttggccat gtagatcagc cggtcaggcg acatggaagg
     7621 ctccttgcgc ggcgacaccg gctgcaattc gctgggggtg ggtgaagacc tcgaatccgt
     7681 cggcgcgcgc aatggcggcg agcgtgatgc cggcggcgtc agccatccgg accgcaagcg
     7741 cggtcggcgc cgacacggcc accattagcg ccgcgcccat ggtcgcggtc ttctgcacca
     7801 tttccaccga gacccggctg gtgagcagca ccatgccgtc gtccgtggct accttctcca
     7861 gcgccagcgc gccggccagc ttgtcgagcg cattgtggcg accgacgtcc tcgcgcaagg
     7921 caatgatgcc gcgcgcctgt gtccagaacg ccgcggcgtg aaccgcgcgg gtctcgatgt
     7981 tgatcttctg cagcgaagca accgactgca tcgcggtcat gatcagttgc ggcgagaacg
     8041 cccgcccgtg cctgacgatg gccgccggac ggagcgcctc cgcgatcgaa tcgatgccac
     8101 acaatccgca gccggtcggt ccggcgatat gtcgccgccg cgcggcgagc cggtccgcct
     8161 tggtttgcgc gagccacatt cgcagctcga tcccgtcatc gagatcgacg acatccagct
     8221 gttcgatttc gtcggccgat tgcacgacgc cttcgctgag actgaatccg accgcgaaat
     8281 cccggaggtt tttgggggtt cccatcatca cggcataggt gccgccgttg taggtcaatg
     8341 cgatcgcgat ctcctcggga atggcgcgcg cgccctcgct ggcgccatcg ccgcgccaga
     8401 ccttgcgact cacgctctga accggatcgt gcatccgcta ctccgccgct tccacgaccg
     8461 gcgcgatgcg ccgggaatgg cgagcctgct cgtcataggc cttttgccat tccgacggac
     8521 cgttcgaggg cgaaatctgc accgccgtga ccttgtattc cgggcagttc gtggcccaat
     8581 cggaatactc cgtggtgatg acgttggcct gggtgtccgg gtgatggaac gtggtgtaga
     8641 ccacgcccgg cgccacgcgt tcggtgatca gcgcccgcaa ggtggtttcc ccggcgcggc
     8701 ttgtgagcct gatccagtcg ccgtcgcgca cgccgcgctg ctcggcatcg tgcggatgca
     8761 tctccagccg gtcctcctga tgccacacca cattcgcggt gcgccgcgtc tgcgcgccga
     8821 cattgtactg gctgagaatg cggccagtgg tcagcagcag cgggaaccgc ggcccggtgc
     8881 gctcgtcggt cgcgatatat tcggtgacca tgaacttgcc cttgccgcgc acgaacccgt
     8941 caatatgcat gacgggggtg ccgagcggcg ccttctcgtt gcagggccac tgcaccgagc
     9001 cgagttcgtc gagcttggca aaggacacgc cggcaaaggt cggcgtcagc gccgcgatct
     9061 cgtccatgat ctcggacggg tgcgcatagt gcatctcata gcccaaagcc ctggcgaagc
     9121 cgagggtgac ttcccagtct tccaggccgt tgcgcggcga catcaccttg cggacccgtt
     9181 gaatgcggcg ttcggcattg gtgaaggtgc cgttcttttc caggaaggtc gatcccggaa
     9241 agaacacatg cgcgtagttc gccgtctcgt tcaggaaaag atcgtggact atgacgcatt
     9301 ccatcgccga caacgccgcg acgacgtgct tggtgttggg atcggactgc aggatgtcct
     9361 cgccctgcac gtatagtccc atgaaggtgc cttcgatggc ggcatcgaac atgttgggaa
     9421 tgcgcaagcc cggctccttg ttgagcttga cgttccacat cgattcgaag gtctcgcgca
     9481 cggcgtcgcc cgagatatgg cgatagcccg gcaattcgtg cgggaacgag cccatgtcgc
     9541 aggagccctg cacgttgttc tggccgcgca gcgggttcac gccgaccccg ggccggccga
     9601 tattgccggt tgccatcgcc agattggcga tcgcgatgac ggtggttgaa ccctggctgt
     9661 gctcggtgac gccgagcccg taatagatcg cgccattgcc gccggtggcg aacagccgcg
     9721 ccgcggcgcg caattcctca gggcttacgc cggtggcaat ggctgtcgcc tccgggctgt
     9781 tcttcggcag cacgacgaat gcggcccagt cctcgaattc gctccagtcg cagcgctcac
     9841 gcacaaaggc ttcatcgacc aggccctcgg cgacgatcac atgggcgatt gcggtgagca
     9901 ccgcgacgtt ggtgccgggc agcagcggca gatgatgctg agcttcgaca tgggcggagc
     9961 ggacgatgtc ggttcggcgc ggatcgacca cgatcagttt ggcgccggcg cgcagccgct
    10021 tcttcagtct cgaggcgaac accggatggc cgtcggtcgg attcgcgccg atgatcatca
    10081 cgacgtcggt atgctcgacg gaatcgaaat cctgggtgcc ggcggaggtt ccgaaggtgc
    10141 tcgacagacc gtagccggtc ggcgaatggc agacgcgggc gcaggtatcg acgttgttgt
    10201 tgccaaaacc ggcgcggatc agtttctgca ccacaaacac ctcttcattg gtgcagcgtg
    10261 acgaggtgat gccgccgacg gcatcgcggc cgtacttcgc ctgaattccc ttgaattttg
    10321 ccgcggcaaa attgaacgcc tcatcccacg atacttcgcg ccagggatcg ctgatgttct
    10381 cgcggatcat cggatggagg atgcgttccc tgtggttggt gtagccccac gcgaaacggc
    10441 ccttgacgca ggaatggccg cgattggcct tgccgtcctt gtacggcacc atgcgcacga
    10501 cttcctcgcc gcgcatttcc gccttgaagg cgcagccgac gccgcaatag gcgcaggttg
    10561 tcaccacgga atgctcgggc tggccgatct cgatcaccga tttttcggtc aatgtcgcgg
    10621 tcgggcaggc ctgcacgcag gcgccgcagg acacgcattc ggagccgagg aagctctcgc
    10681 tcatgccggg cgagacccgg ctgtcgaagc cgcgcccgga aatggtcagc gcgaacgtgc
    10741 cttgcacttc ctcgcaggcg cgcacgcaac gcgagcacac gatgcatttc gatgggtcat
    10801 aggtgaaata gggattggat tcgtcctttg gcatccaggc cgggttggcg gcgccgtcgg
    10861 atttggcgaa gacgtgattc tcgccctgat agccgtagcg cacgtcgcgc aggccgacgg
    10921 cgcccgccat gtcctgcaat tcgcagtcgc cgttggccgc acaggtcagg cagtccagcg
    10981 gatggtcgga gatgtagagc tccatcacgc ccttgcgcag ctttttcaac cgctcggtct
    11041 gggtatggac cacgaggccg gccatcgcag gcgtggtgca ggaagccggc gtgccggcgc
    11101 ggccttcgat ctcgatcagg cacatccggc acgatccgaa cgcgtccacc atgtcggtgg
    11161 cgcaaagttt tgggatttgc gtgccggctt ccatcgccgc gcgcatgatc gaggtgccct
    11221 cgggcaccgt gacggtttgc ccgtcgatgg tcagggtgac catcgcttcg gagttgaagc
    11281 gcggcgttcc gaaatcgatc tcgtgcacaa gcgacatggt gatgatcctt tattccgcag
    11341 cttgcaggcg cggcggggcc gcaccaaaat cttcccggaa atgtttcagt gcgctcatga
    11401 ccgggtaggg cgtgaagccg cccaacgcgc agagcgaacc gaacttcata gtgttgcaga
    11461 ggtcctcgac cacggcgatg ttctcggcca cgcggtcgcc ggcgcggatc ctgtcgatgg
    11521 tctcgacgcc gcgggtcgag ccgatccggc agggcgtgca cttgccgcag gattcgatgg
    11581 cgcagaactc catcgcgaag cgcgcctgct tcgacatatc cacgctgtcg tcgaacacca
    11641 cgatgccgcc atggccgatc aggccgtcgc gcgcggcaaa agcctcatag tcgaacggcg
    11701 tgtcgaacag cgcgcgtggg aagtatgcgc cgagcgggcc gccgacctgc accgcgcgaa
    11761 ccggacgtcc cgtatcggtg ccgccgccga tatcatcgac cagttcgccg agcgtgatac
    11821 cgaaggcgac ctcgaacaat ccgccgcgct tgacgttgcc tgcgagctgg atcggcatgg
    11881 tgccgcgcga gcggcccatg ccgaaatcgg cgtaggcctt ggcgccgccg gcgaggatga
    11941 aggggatcgc cgcgaacgac agcacgttgt tgatcacggt gggcttgccg aacaggccct
    12001 tatgcgccgg cagcggcggc ttggcgcgga cgatgccgcg gcggccttcg aggctttcca
    12061 gcagcgaggt ttcctcgccg cagacatagg cgccggcgcc gacgcggact tcaagatcga
    12121 agctgtggct ggagccgccg atgcgcgcgc cgagatagcc ggcgcgcgtc gccgccgcta
    12181 tcgccgcatt catcgccgcg accgcatgcg gatattccga gcggatatag atgtagccct
    12241 tggtggcgcc gaccgtgatg cccgcaatcg tcatgccctc gattatgaca aacggatcgc
    12301 cttccatgat catgcggtcg gcgaacgtgc cgctgtcgcc ttcgtctgca ttgcagacga
    12361 cgtatttccg gtcagcgccg gtctgcgcca cggttttcca tttgatgccg gtcgggaagc
    12421 cggccccgcc gcggccgcgc aatccggaaa tcgtgacgtc ggcgagaatg gcgtccgacg
    12481 tcagcgtcag cgcgcgctcc aggcccttgt agccagcatg ggcgcgataa tcctcaaggg
    12541 agcgcggatc gatcacgcca cagcgggcga acgtgaggcg ggtctgccgc ttcagccagg
    12601 ggatctcgtc cgcgacgccg agccacaggg gatgcgcgcc gccagatatc atcgcatcga
    12661 gcacggaacc ggcgtcaaac tcggtcaccg gaccgaacgc gatgcgccct tgagatgtgg
    12721 cgacctcgat catcggctcc agccaataca ccccccgcga gccggttctg acgatctcga
    12781 tgtcaatgcc gcgcttgccg gcggccaggc ccagcaccaa tgcgacttca tcggctccga
    12841 ccgcgacagc acccgcgtcg cgggatacaa aaattcggag gctcatcgct gcgcctccgc
    12901 gatcagcgca tcgatgcgcg cttcgttcag acgcccgacc acgcggccat ccagcatcgc
    12961 cgacggcgcc gtcgcacaga ggccgaggca atagatcggc tccagcgtca cccgctcgtc
    13021 cgccgtggtg tttccaaggg tgatgccgag cctcgcttcc gcgcgcgccg ccagcgcatc
    13081 gccgcctgcg gcctgacagg cttcggcgcg gcacagcttc aggacgtgct tcccggccgg
    13141 cccgcgccgg aaatcatgat agaaggtgaa gacgccgtgg acctcggcgc gggacaggtt
    13201 caatgccgat gcgatcatcg gcaccgccgg ctccggcacg tagccgaacg cctcctgcag
    13261 ggcgtgcagg atcaccagcg tcgcgccttc aaggcggttg tgctcggcga tgatttcagc
    13321 gccgcgcgcg gcattccatg gttcgtaaac cggcatcatc cacgttctct gtgagctgct
    13381 tgttggcggc caatgagaat tggaattgat caagaaatca ataaagctgt ctcatgctgc
    13441 gatagggaaa tgcgatcgat agggttataa gatcaggcca taggaaaatg gaatgagtcc
    13501 atgtgagcgt ggcagcggtg ctcatttgtc gcgacttttt gcatctttgt cgtttgagat
    13561 cgtgatattc gtagcgctgg aacggtgagg ctctcttgat cgacaagctc gaacttctgc
    13621 tcgcgctggc aaaggagcgt cacttcggac gcgccgcgga agcctgcggc gtgacccagc
    13681 cgacgatgtc gaccagcctc aagcagctcg aggaaatcct aggcgtgatg ctggtgcagc
    13741 gcggctcgcg gtttcagggc tttacgcccg aaggcgaacg cacgctcgac tgggcgcggc
    13801 gcattgtcgg cgacgcccgc gcgatgcggc aggaaatcaa cgggctcaag gacaagctat
    13861 ccggcgagat caggatcgcg gcgatcccga ccgttctggg catggtggcg tcgttgacga
    13921 cgccgtttcg cgcccgctac ccggatgtgc tgttccgcat cgtgtcgtgc acgtcggccg
    13981 acgtgttggg cctgctggaa aatctggagg tcgatgccgg gttgacctac atcgagaacg
    14041 aaccgatcgg caaggttcgc accataccgc tctatgacga gagctatcgc ctgctgaccg
    14101 cgcccgacgc catgttcggc gatcgcgata ccgtcacctg gaaggaggtc gggcaggtgc
    14161 cgctgtgcct gctgacgccg gacatgcaaa accggcgcat tatcgaccgc gcgctgcgtt
    14221 cggtcggcgc cgaggcgacg ccgacgctga cctcgaactc tctgctggtg ctttacaccc
    14281 atgtgaagac cgggcgctgg gccagcgtga tgccggcgaa gctcgcggaa acgctcggct
    14341 tggcggacac cgtccggagc attccgattg tcgatccggt cgtcacctac agtgtcgggc
    14401 tggtgattcc gcagcgcgac ccgatgacgc cgctgattgc ggcccttgtc cacaccgtcc
    14461 gcgaagtcgc cccgacgctg gaatcctgaa tcaagccgcc acggacatct tcgctctggc
    14521 cttgccggcg tcgcgcggca gcgacacgca gaccacggtg ccggcgccga gtttcgagcg
    14581 cagccgcatc gatccgccat gcaggttggc gagcgacctt gcgatggcaa ggcccagccc
    14641 cgagccgtga taggtcttgg tgagctgact ctcgacctgc tcgaacggcc ggccgagccg
    14701 cgcgagcgaa tgcggcgcga tgccgatgcc ggtatcggca atcatcagca ggatggaatc
    14761 gctgagcact cggctgcgga cgatcacctt gccgccctcg ggcgtgaact tgaccgcgtt
    14821 ggacagcagg ttgacgatga tctgcttgat ggcgcgacgg tccgccacca cggaaatggg
    14881 tccctcgatg tccgcatcga gcaccagatt cttgtcgtcg gcgcgccccg acaccacccg
    14941 cagcgattcc gccagcgtct tcgacagatc aagcgattcc atgtcgagct tcatccggcc
    15001 agcctcgatc ttcgacatat ccaggatgtc gttgatcact tcgagcaggt aatgcccgct
    15061 ggtgaggatg tcgtggcagt attcctggta tttttccgat cccagcgttc cgaacatgcc
    15121 gctttccatg atctcggaaa atccgatgat ggcgttgagc ggcgtgcgca actcgtgact
    15181 catattggcg aggaattttg acttggtctg gttggcttcc tcggcgcggt ttttctcttc
    15241 ggagtatttt tcggcgaggt cggcgagctc gagggcttgc tgttcgagcg cggtctgcga
    15301 acgtttcaga tcgatcacgg tggcgcgcag gcgaaggtcg ttgtcgacca gtttctgttc
    15361 atgttccttg atgcgggtaa tgtcggtacc caccgagaca tagccgccgt ccttggtgcg
    15421 ccgctcgctg atatgcagcc agctgccgtt gtcgagttgc gcctcgaagg tgcgcgcgcc
    15481 cggcggctgg gtgccggctt catgcagcct ggtgcggacc tccggcatgc tgccgacttc
    15541 gatcacggtt tcataggacg tgcccggcgt cacggccgaa tccggcagct tgtggagccg
    15601 ctggaagtgg gagttgcaga gcaccaggcg gtcttccgcg tcccacagca cgaaagcttc
    15661 cgggatggtc tcgatcgcat cgcgcagccg caggtcggcc tccacggttt tctcggcgag
    15721 gcttttttgc tcggtgatgt cgacggcgat gccgatcaga tgcaggccgg catcggcctc
    15781 gccctggctg agctcacagc ggacccgcag ccagatccag tggccgtcgg tgtgctgcat
    15841 gcggaaggtc tggtcgatat ggttgatttt cgaggagatg agctggtcgg cgatcgcgaa
    15901 cagatcgatg tcgtcggatt tcaccagcgc gttgacctcg ccgaaagtca aaaggtcgct
    15961 gcgggtgtcg aggccgagca gcgtgaacat cgattgcgac cagaagattc tgccgcgcga
    16021 caggtcccag tcccacaacc cgcagcgccc gcgattgagc gcggtatcga tccggccgcg
    16081 caccgcgtca ttgatgagat cgccttcgcg cgcacgggtc gattgccagt ggaatgcaaa
    16141 gcccaggatc agcacgacaa agccggtggt ggcggaaagc gtcaccgaca aggccgcgtc
    16201 cgaccgccac agcgagtcga ccttttcctg gacgatgatg acgtgaccgg gcagcgattt
    16261 gacgacgtgc tggaccgcca gcgcgacgcc gccgctcggc aggatcatgt cggtgatggc
    16321 accctggtga gcaggtgctg ccagcaattg cgccgtgctg atggcgtcga gaatccggtc
    16381 ggggtcgccg aggcttgttt cgacgggaac gcgggcaaga atgcgctggt ccgcgccggt
    16441 gacgatgacg tgccggccgg ccgcgatgcc ccacgacgga atgaggccgg gcaccaggcc
    16501 ctgcaggcgc tcgacggtcg ctgtgcgatc ctcccgggca gacgcaatgc ggtcaaggcg
    16561 ctcggccagc aagtcggcca gcgcggcgag gtcccgcttc atggacgcgc gctgttgccg
    16621 gttctgatcg accacctgca cgaaggcgcc aagacagatg gtgatcagga acgctatgat
    16681 cagcgttggc acagcgcggc gaagtgcagg ctccgctatc aacagccgat gataggccgg
    16741 tttcgcgatc gactgcgcca accccttgat tgaatcggat tgaacgcacc cggtcgccgc
    16801 gtgcgcgcgc gccatgccgt aggcccctcg gagatctctt tgcaccttct tcgaaacaac
    16861 cccagtcact ttcgaatcaa ggcgatttga atccagtttt ggcgggctgt cgagagtcaa
    16921 cgaatcgtta atgcaaataa atcttgtcca ctgcagaatt ggaactccga aatgcggtgc
    16981 aaagtgagtc cgaaagggga acgcgccggc gatttcagat tttactgcgc gtccggcggc
    17041 tcggcgtggc tgatcactcg tttcacggtc gggaacgcgc ggcgcaacgc gcgctcgatc
    17101 gcatcgacgt tctcgtgaac cttgatcacg ctcatcgacg gcgcggcgcg acaatggaag
    17161 tttacgatct cgccggcgtc ggtattgcgc acccgcacgc tgtggatgtc atggatcgcg
    17221 ccatcggcgg caaagcgtga caacgcggtc ttgatggtat cgacgcggtc gggcgtggcg
    17281 tcggcgccga gcggtaattc cggctccagc ggctcgatgt gggtgtcgac ctcgacgtcc
    17341 tcaccgaatt cgtcgcggat gttgcgctcc agctcatggg cgatctcgtg ggcctggtta
    17401 agcgccattt ccgcgtcgac ttcaaggtcg atgctgacgg tcagcttgcc gccgagattg
    17461 tgcaccgtga catggtggat ggcgagcccg gaattgcggg cgatcaccat gatgcgttca
    17521 cgtatgctct cgttgttacg cgccaccggc acggcggtga acgtcagatc ggcgtcgtcg
    17581 agtactttgc tgaccgcctc ctgggccttt cgcttgattt cctcgacccg gtcgatcgga
    17641 taggtgcgcg gcacctgcac gatggcgtca atgaagtgcg tcgagccgac catccgcatc
    17701 ctcaggcgtt cgacatcgac cacgccgggc accgcccgga tcgcggcgat ggcttcttcc
    17761 gacgcgcctt cgggcgcgcg gtcgagcaag gtttccacgg tggagcgcgc cagccgcagg
    17821 cccagcgacg agatcacgat cgcgacggcg atcgcggcgg cggcgtcccc ccaggcatag
    17881 cccagtcccg acaatgcaag tccaaccatg acggcaatcg atccaagcac gtcggatgcg
    17941 aaatgcagcg catcggcggc cagcgcctgg ctcttggtgt cgcgcgcggt gcgatgcagc
    18001 gcccgggcac gccagaaatt gacggcgatg tcgatcagca ggacgatgaa cgggatggcc
    18061 gagatcgtcg gcggtggcgc accttcgcgc aaccggctgt aggattcgac cagaatgcca
    18121 cccgccagca cataaagcat cgcgatgacg ccgagcgccg acaagctttc aaatttgcca
    18181 tggccgtaat gatgttcgtg gtccgcgggg cgatcggaaa cccgcaccac gagccaggtg
    18241 acgacggtcg caaccacgtc gaccgaagag tgcagcgctt cggaaatcag cgccagactg
    18301 ccgatggcga taccgaccac gaatttcgcc gccgccatgc cggagctggc gaaaatcgaa
    18361 atcgccgcga cgcgggattt gacgctgtga tcgggatgaa tcataggcgg ggtttagcag
    18421 cccgtcatgc aacattaaag tgttgatcaa gtgcgcaaac gccaatcact tgcagcaacg
    18481 actttgttgt gaactattcc gattttaaaa tcttcccgtg ctcggtcaga ccccgtacgc
    18541 cgccaaggcg tgaggcttca tcgccagact attgcagccg atccagacga agtcgctgac
    18601 gatcgtccgt cagacgccac gccgcgagat aggatgcggc caatgccgat aatccgctgc
    18661 cgcccgacaa caggaacatc aggaggatct gatatttgac ggcctccagc ggatcgaggc
    18721 ctgcgagaat ctgtcccgtc atgatgcccg gcaacgtcac gatcccggcg gccgacattt
    18781 ggttgatgat gggcaacagg ccgcgccgga tggcctcgcg caccagcggc gcgatggcct
    18841 cgcgatagct atcgccgagc gacagtcgcg cttcgatggc ttgtcgctcg cgccggaccg
    18901 accccaaaaa gctatccagc gacaggctgg cggaattcag aacattgccg agaatgatgc
    18961 ccgccagcgg gatcgcataa tgcggatcga accagggctg cggccggatc gccgtggtca
    19021 atgcaaggat cgacgtgaca aacgtggcaa agccgacgct gacaaacccg atgacgagat
    19081 ttgaatagtc cttgaaccgg cgttccggcc gggccgccac ctcgcgcgcg gccaccagca
    19141 ccatgacgag aatgaggagc agtgtggccg ccgggttgtt cagcgtaaag atgaaacgca
    19201 gcacatagcc gattgcgacc agctgcacga ccattcttgc cgcggcgatg gcgatttgcc
    19261 gatgcagccg aagcttgagc aaaattgaca gcgccgcatc gagaatgatc agggaggcgg
    19321 cgatggagat gtcaaacggg ctcagcagga tagggttcat ggcgtctgca gctgtcccgc
    19381 caccatgcga tagcgcaaat cccccaggcg ttcggcctgg tttggatcgt gagtcaccag
    19441 caggatggat gtccccgttg cctttcgctc ccgcagcagc gcttccacct tggcgaccga
    19501 ctcctcgtcc agggggccgg tcggttcatc cagaagcagg acgggtggag acggcagcag
    19561 ggccctgaca agcgagaagc gctgcttctc gccggtggac agttgcgcga ccggtgcgtc
    19621 cagaagatcg gcgcgaagcc cgaggcgggc tgccaaggct gcgacctcgc ttcgccggct
    19681 tacgtcgaaa tgttcgatca ccctgtccgc ccaccaaccg gattccgcgg atacgtaggc
    19741 tgcccgtctt cgccattgcg gggcgggcat cgagccgcgt tctctcccgt tcagccagac
    19801 cttgccttcg ttgggatcga ggtcggcaat catgcgaaga aaaaggctct tgccggatcc
    19861 cgacggaccg gtaatggcgg cgcacgcgcc ggtgccgagg ttcagttcga acgggccggc
    19921 gaacgcgctg cggagatctt tgacaaggag tgccgttgcc atcgaattgc cttgtctgca
    19981 gcggccctgg ctgtgttgcg agatttcccg cacccgcagt caagactaca ccaattctcg
    20041 acgccgcgga atttgccgag tttgaccctt ggcggaagtc caccaacgct cagggatagc
    20101 cggcaccggg aatttcgacc tcggccacgt cgatgcgggc agacgattgg cgttgccgga
    20161 gctgttcggg cgaggtcgcg gcactcaggc agaacagtgt ccgccgctcc gggccgccga
    20221 gaacacaggc aatggcgcgg cggccgggca gttcgattcg ctgcaactcg cggccgtcgc
    20281 ggttgacccg aatgaaggcg tcctcgccga aggatgcgac ccagacggcc ccctcgtgat
    20341 cgaggcaaat gccgtctcga gctttcatgt ttccgaatcg gcgacagaaa ttcaaaccgc
    20401 cgtcggcctc gatgtcatac tcggccaggc attcgccatc catttcggcc acgacgagcc
    20461 gtttgttatc ggccgagacc gcaatgccgt tgggaaaccg caatccatcg gccacgacgc
    20521 gcctctcgcc gccaggcatc accagaatga tgcggccgac agggccgcga tcgggcggcg
    20581 gtggcaaatc gaagccgaga tctccgacat aagcgcgccc cagcccgtcg acgatcatgt
    20641 cgtcgatcgt gcctgtcgcg atccccgaca ggtcggcata gagcgaaagc tcgccgtcgg
    20701 cgtagctcat cagacgcttg cggaacatcg tcagcacgac gagctttcca tccggcaaga
    20761 cgccgaggcc gcagggcgtg tcgtcctcga acttcgcgtg ctgttcgcac gcgccggagg
    20821 cgtcgagact gaggagtgtc cgctccatgc agtcgacgaa ccagagccgg cccaagtgcc
    20881 agcgtggccc ctcgaaatag cgtcccccgt cgagcactcc acttggcatt ggccggcgcg
    20941 gcgtcatagc gtcacgacga tcttgccgag atgcttgttg gcttccatgt gctcgaacgc
    21001 cttgccgatc tcgtcgaatg cgaacacctt gtcgatcggc aattgcagtc tgcgctgctc
    21061 caccgctccc cagatatcct tcttgacttc gccaaagatc tcgcggattt cctcgatcga
    21121 gcgggtgcgg aaggtgacgc cgatataggt gatgcggcgc gccgcatgca ggtcgaaatt
    21181 gaaatcggca tgggtgccgc cgagcctgcc gacattgacg atgcggccct tgatcctggt
    21241 ggccgccaga ttctggctcg cgaccttgcc cgagacctga tcgacgatca ggtcgacacc
    21301 ttcgccgccg gtggcttcca gcacctgatc gacccagccg ggatcgctgg aatccaccgc
    21361 aaggtcggcg ccgaattcct tcaaccggcc gcggcgcgtc gcatcggtcg aagatccgat
    21421 cacgatcttg gcgcccttga gttttgcgat ctgcagcgcc atcaggccga cgccggaact
    21481 cgcgccttga atcaagacgg actggccggg ctgcaacgcg ccgttggtca cgaccgcatt
    21541 gtgcatggtg gccagcgcga cgggcagggt ggccgcgtcc tcgtagctca tattggcact
    21601 ggcaggaatg tgaaacagcc ggccgtgatc ggccagcgta tattccgcaa acgcggcgct
    21661 gcctgatccc atcaccctgt cgccaacctt gacgcccttg gcctcaggcc cgacatcggc
    21721 gatttcaccg gcccattcca tcccgagcac cgtgccggcg ccgccggcgc tgccatgtac
    21781 atggcccttg gtcatgccga ggtcggcgcg attgagcccg caggcgcgga cgcgaaccag
    21841 cacctgtggg cctttcggag cgggcttggc gacttcggtg atttcagcgc cgttggcgcc
    21901 gtagacatag gccttcatga aatcattctc tgttagtggg tgagacgaga ccgcctattc
    21961 cgcggcctga cggcgggcac cggacatcat gccttcgagc cggctgcgaa tgatggcttc
    22021 cgcgtcacgc atgatgcgcg acaccagttc ggcgcagctc gggatgtcat ggatcaaccc
    22081 ttgcacctgg cctgccgacc agatgccttc gtcggcgtcg cctgtggcgt agaccatctt
    22141 gccgcgcgcg ccggccacga gttcgcggac ctgctcgaag gtcgcgcctt ctttttccat
    22201 ggcgacgacc tgggtgctga tcgcgttcct ggcgacgcgc gaggtgttgc gcatggtgcg
    22261 gaaaatcagc tcggtctcgc gctcgtcatt ggcgacgatc ttttccttga tcagctgatg
    22321 gatcgggctt tccttggtgc acatgaagcg ggtgcccatg ttgatgccct cggcgcccag
    22381 cgccagcgcc gcgaccaggc cgcggccatc gccgaagccg cccgaggcga tcatcgggat
    22441 ttttaccttg tcggcggcga ccgggatcag gatcaagccc ggtgtatcat cctcgccggg
    22501 atgccctgcg cactcaaaac cgtcgatcga gatcgcgtcg acccccatcc gttccgccga
    22561 cagcgcgtgg cggacgctgg tgcatttgtg cacgaccacg acgccgtgct tcttgaactc
    22621 ggtgacgtgt tcctgcggct tgttgccggc ggtttccacg atcttgatac cgctctcgat
    22681 gatggcctgg cgatattccg cgtagggcgg tggcttgatc gagggaagaa tggtgaggtt
    22741 gacgccaaac ggcttgtcgg tcaggctgcg gcagcgcgcg atttcgcgtg tcaggtcgtc
    22801 gggcgtcggt tgggtcagcg cggtgatgaa gccgagcgcg ccggcattgg cgaccgccgc
    22861 caccagctcc gcgcgtccta cccattgcat gccaccctgc acgatcgggt gttcgacgcc
    22921 gacgagctcg gtaaaccgcg ttttgatcat cgccagccct ctgtttcccc ggcgcggagc
    22981 gcccgcgcac gttctatttt gtgaactttc gcggggaagg atgccgtccc cttccggaaa
    23041 agtccacggg gcaaggcaga ggacagcgcc acgcgttcat gcaaaaacgg ggatttttcc
    23101 gtcgcgggac ggacatttcc ttcatacagt cccgcgattt tcgcgcgctg tgcaaccagc
    23161 gccagcaccg catcgagagc cggtgtcgga atccgtgtca atcgccccat ttcctgaacc
    23221 acagacacca gcgggtcgat ctccatcggg cgtccgcgtt cgagaccctg cagcatcgag
    23281 gtcttgtggg cgcgcccttc cgcgcgccgt cgatccgccg ttcgacatcg acccggaatt
    23341 tgacgccaaa cgtctcggcg atcgctgctg ctggtctccg atcagcaatt tcaatccgcg
    23401 ctcgcgcatc gcggcaagat gcgcgccgcg ggcaacgagg ctgacgtcgg cgcccttgcg
    23461 cgtaagctgc acaccgagac atccaccggt cgcgccggcg ccgtagatac atattttcct
    23521 tcggatacgg gtgatggaaa tttccgcaca ggacacgcgc tggcttggag cgaaagctca
    23581 aagccagggt aaaatcgcgg ccgccgggaa cgggagccgg tttcgtcagg ccgcggaggc
    23641 ggatcgtgcc gcgcccgttg cctggtcgtc gaacgcggtt gagatgtcgc ggatgctgac
    23701 aacgccaacc aggctgtaat cgtcgatgac aggcagatgg cggatgtggt tcctgttcat
    23761 gagatgacga acatgttcga gcgtatcggc cgaactgcag gagaccagct gtcccactga
    23821 aatcaattgc gagactttca ggttgacccc ggccgcgccg tgttcggcaa tagcgcgaac
    23881 gacgtcgcgt tcggtaaaca tgccaaccgc ggtattgcct tcggtccgga caacgtcctt
    23941 gaccacaagc gcgctgatat tgctggcgcg catcagctgg gcggcaatgg cgacggtttc
    24001 gttcattcga accgtcgcaa cgcgggcagt tttcttgcgc aggatgtctc cgacttgcat
    24061 cgcaacctcc ttcaaatgag ccgcagggcc actttggcaa taactagatt ggtatacatt
    24121 atgccaattg tcaatttgaa atcgcggttg gcatgccacg ctcgaaagca aagaaagtgg
    24181 cagtaagact gacgttctaa agcattctgt gttgcttcca tcagggaaga cgcgatctgc
    24241 caggaggtgc gcaaggcccc aagtatgcca gcgaccgcgg ctcgcgcaaa aaaaagcgcc
    24301 caccccttgg atgagcgcct ccttttcagc aggcggtgca ggacgggtta atcgtactga
    24361 gtcagaaggt cgcagccgtt gcggccgaga atcaaataat tgaagggatt cagattttgg
    24421 cacgtggagg gccagatgaa tctcaagcat gtcggcgaca gtgaagcacg gtttgcggcg
    24481 tatgtg
//
LOCUS       DATNOI010000350         4441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1084890,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000350 DATNOI010000000
VERSION     DATNOI010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084890"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VN968_13970"
     CDS             complement(<1..1199)
                     /locus_tag="VN968_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL30378.1"
                     /translation="MSDAQAQFSVLQQTADPAVADAIQLLIEQGEDHELNRINLLDFS
                     GRTGLDEERVISGFLHASRLGLFDLTWNVLCPGCGGVLDAHSTLKSLRHDDYHCGLCA
                     CGYEASVDEQVEVAFTVSPRVRRIAAHDPNALPLWEYFKQIFWSSGVDLDRESFASLS
                     NDVVLDAIELPPHEKAALSLQLPAQFIIVFEPVTHAAQFIDVQGEPTEECRELSLTYN
                     KIQAPVGTVTLRPGPLRLSLDNQTDVRVLPSVLIAADALHHLIGKRKPFLTAKRMLTN
                     QTFRDVYKADNLNIDQRLKITSLTFLFTDLKGSTALYERVGDLVAFDLVRAHFHALLE
                     IIASEKGAVVKTIGDAVMATFVKPEHALAAGLRMRAAMEALNAERGTKDLVVKIGIHE
                     GPCLAVML"
     gene            complement(1246..2634)
                     /locus_tag="VN968_13975"
     CDS             complement(1246..2634)
                     /locus_tag="VN968_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXL30379.1"
                     /translation="MSIAEVLDMPVPRQPLVPPSPPRASETMTFFGRLAAMRVSVLGI
                     WGQRAYEEDIIQGRFFGRSSFILNTPDTIKHVLVDNYENYTRTPAAFRVLRPVLGEGV
                     LLAEGRAWKHQRRTLAPAFTPRAVGTLVPLMLAATDETIAKLQAACGTPVDLREAMQR
                     MTLEIAGRTMFSFEMNRHGPALRDFVMEYGARLARPHVLDVLLPQGWPSPQDFPRARF
                     RKRWTSFVAMLMAERRAAGKHEGAPPSDLFDLMGAARDPETGDAFTDEQLGDQVATMI
                     LAGHETTATALFWSLYLLALDSTSQDQLAAEVQNATVNGALDVERLKFARAVVDETMR
                     LYPPAFLIARAAAGRDTIAGGPVKKKDVILIAPWLLHRHEKLWRDPNAFIPARFMPGT
                     PPPDRFAYLPFGVGARVCIGAHFALVEATLALARMIGAFRIELLDKAPVLPVGVVTTQ
                     PDRSPMFRITPR"
     gene            complement(2758..4131)
                     /locus_tag="VN968_13980"
     CDS             complement(2758..4131)
                     /locus_tag="VN968_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HXL30380.1"
                     /translation="MSISRRTILKGATILPVMANSFGFCAAAQTSSADPGTTEVPPVL
                     FVHGNGDHAALWITTVWRMESNGVPRDRMFAINFTDPLARTDDSVPQPNRSSTEDARR
                     ELGEAIKELKRRTGASRVALVGNSRGGNPIRSYIKGGGAADVSHAVLCGVPNHGVYDW
                     DDGLGGEFNGRGPFLRGLNQGESEVTPGTSFLTLRSDGLDKYAQADGRFVGKPGTPTG
                     ITAEGPALKGATNLVLGAVDHRETAFSPRAFREIYKFIAGREPDRIEITPEAQVKLSG
                     LVTGTPGGVQTNRPVAGASVEIYRVSPDTAERTGGAIHASQSAEDGRWGPAEVDPSWY
                     LEIVLTSVGSTTTHFYRSPFPRSSDIVHLRAARPSGPADAGAGAVVLMSRPRGYFGLP
                     RDVVLFDGKEPSDVKPGVPTDSVSTLRLPAADIGRPVVALFNAERIVVRARPASENRI
                     TVAELTY"
     gene            4281..>4441
                     /locus_tag="VN968_13985"
     CDS             4281..>4441
                     /locus_tag="VN968_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL30381.1"
                     /translation="MTDQPKPKMFAADSVTAPLRHSVFRRIWLASLLSNFGLLIQGVG
                     AAWAMTQMTS"
BASE COUNT          861 a         1415 c         1382 g          783 t
ORIGIN      
        1 agcatgaccg cgaggcacgg accttcatgg atgccgatct tgaccacgag gtccttggtt
       61 ccgcgttcgg cattgagcgc ctccatggcg gcgcgcatcc gcagacctgc ggccagcgca
      121 tgctccggct tcacgaatgt cgccatcacg gcatcgccga tcgtcttcac gacagcgccc
      181 ttctcggacg caatgatttc cagcaaggca tggaaatgcg cgcggaccag atcgaacgcg
      241 acgagatcgc cgacccgctc gtatagcgcg gtagaaccct tgaggtcggt aaacaggaac
      301 gtcagcgagg taatcttgag gcgctgatcg atgttgagat tgtcggcctt gtagacgtcg
      361 cgaaacgtct ggttggtcag catccgcttg gcggtgagga acggctttcg cttgccgatc
      421 agatgatgaa gcgcgtcggc ggcgataagc accgacggca gcacgcgaac atcggtctga
      481 ttgtcgagcg acagccgcag cggcccgggg cgcaaggtca cggttccgac cggcgcctga
      541 atcttgttgt aggtcagcga tagctcccga cattcctcgg tcggctcgcc ctgaacgtcg
      601 atgaattgcg cggcatgcgt caccggctcg aacacgatga tgaattgcgc cggcaactgc
      661 agcgacaacg ctgccttttc atgcggtggc agttcgattg catccaacac cacgtcgttg
      721 gacagcgacg cgaacgattc cctgtcgaga tcgacgccgg aactccagaa gatctgcttg
      781 aaatattccc agagcggcag cgcattggga tcatgcgccg cgatccgcct gacccgggga
      841 ctgaccgtga aggcgacctc gacctgctcg tcgaccgagg cctcataacc gcaggcgcat
      901 aatccgcaat gataatcgtc gtgacgcagc gatttcaggg ttgagtgggc gtcgagcacg
      961 ccgccgcaac cgggacacag cacgttccag gtcagatcga acaggcccag ccgcgatgca
     1021 tggaggaagc cggagataac ccgctcctcg tcgagacctg ttcgccccga aaaatccagg
     1081 agattgatcc ggttgagctc gtgatcctcg ccctgttcga tcagaagctg gatcgcatcg
     1141 gcgaccgcag ggtcggcagt ctgttgcagc accgaaaact gggcttgggc atcactcatg
     1201 acgcgagttt agagcagatt acgcgtgcgt ccaagcgatg cattctcacc ggggcgtgat
     1261 gcggaacatc ggcgagcgat cgggctgtgt cgtcaccacg ccgacgggaa gcaccggcgc
     1321 tttatcgagc agttcgatcc ggaacgcgcc gatcatcctg gccagcgcca atgtggcttc
     1381 caccagcgcg aaatgcgcgc cgatgcagac ccgcgccccg acgccgaacg gcaaataggc
     1441 aaaacgatcg ggcggcggcg tacccggcat gaatcgagcc ggaatgaacg cgttcggatc
     1501 gcgccagagc ttttcatgcc gatgcaacag ccacggcgcg atcaggatga catccttctt
     1561 cttcaccggc ccgcctgcga tcgtgtcccg gccggccgcg gcgcgcgcaa tcaagaaagc
     1621 cggcgggtag agccgcatgg tctcatcgac caccgcgcgg gcaaatttca accgttcgac
     1681 atcgagcgcg ccgttcacgg ttgcgttctg tacctcggcg gccaattggt cctggctcgt
     1741 ggaatcgagc gcgagcagat agagcgacca gaacagcgcg gtcgcggtcg tctcatgacc
     1801 ggcgaggatc atcgtcgcga cctgatcgcc gagttgttcg tcggtgaagg cgtctccggt
     1861 ctccggatcg cgcgcggcac ccatcaggtc gaacagatcg ctcggcggcg cgccttcgtg
     1921 cttgccggcg gcgcggcgtt cggccatcag catagccacg aaactggtcc atcgcttgcg
     1981 gaagcgggcg cgggggaaat cctgcgggct cggccagccc tgcggcagca gcacatcgag
     2041 aacatgcggc cgcgccagcc gcgcgccata ctccatgacg aaatcgcgca acgccgggcc
     2101 gtgccggttc atctcgaagg aaaacatcgt gcgtccggca atctcgagcg tcatccgctg
     2161 catcgcctcg cgcagatcga ccggcgtgcc gcaagccgcc tgcagcttcg cgatcgtctc
     2221 gtcggtcgcc gctaacatga gcggaaccag cgtcccgacg gcgcgcggcg tgaaagccgg
     2281 cgccagcgtc cgccgctgat gcttccaggc acggccctcg gcgagcagca ccccctcgcc
     2341 aagcaccggg cgcaacaccc ggaaggccgc cggagtccgg gtgtagttct cgtaattgtc
     2401 gaccagcaca tgcttgatcg tatccggtgt gttgagaatg aaactgctgc gcccgaagaa
     2461 gcgaccctgg atgatatctt cttcgtaagc gcgctgcccc cagatgccga gcacgctcac
     2521 gcgcatcgcc gccagccgcc cgaagaaggt catggtttca gacgcgcgcg gcgggctcgg
     2581 gggcaccagc ggctgacgcg ggacgggcat gtccagaact tcggctatgc tcatttgacg
     2641 gcctcgcgat ctcaaaccta gatatgatac gggcgacgct tcgcaatcat atcaggtccg
     2701 aaatccgggg cgccatgaag gcgctctgac ccaaaaatga caactggaaa atgatagcta
     2761 gtaagtcagc tcggcgaccg taattctgtt ttccgatgcc ggccgcgccc gcaccacgat
     2821 ccgttccgcg ttgaacagcg ccaccacggg acgcccgatg tcggcagccg gaagccgcag
     2881 cgtactgacc gagtcggtcg gtacgcccgg cttcacatcc gacggctcct tgccgtcgaa
     2941 cagcacgaca tcgcggggca agccaaaata gccccgcggc cgcgacatca gcacgacggc
     3001 gccggctccg gcatcggcag gccccgatgg gcgcgcggcg cgaagatgca cgatgtcgga
     3061 ggaacgcgga aacggcgagc ggtagaaatg agtggtggtc gaaccgacgg atgtgagcac
     3121 gatctccaga taccatgatg gatcgacctc ggccggcccc cagcgtccat cctcggcgga
     3181 ttgcgaggca tggatcgcgc cgccggtgcg ttctgcggtg tcgggcgata cgcgataaat
     3241 ctcgaccgac gcacccgcga ccggacgatt ggtctgaacg ccgcccggcg ttcccgtcac
     3301 gaggccgctg agtttgacct gcgcttccgg tgtaatttcg atccggtccg gttcgcggcc
     3361 ggcgatgaat ttatagatct cgcggaatgc gcgtggagaa aacgccgtct cgcgatggtc
     3421 gacggcgccg agcacgagat tggttgcgcc cttcagcgcg ggaccttccg cggtgatccc
     3481 ggtcggtgtg cccggcttgc cgacgaagcg gccatctgct tgggcatatt tgtcgagacc
     3541 gtcgctgcgc aatgtcagga acgaggtgcc cggcgtcact tcgctctcgc cctggttgag
     3601 cccgcgcaaa aacgggccac ggccgttgaa ctcgccaccg agaccatcgt cccaatcata
     3661 gacgccgtgg ttgggaacac cgcacagtac ggcgtggctg acatccgcag ccccgccgcc
     3721 cttgatataa ctgcgaatcg gattgccgcc gcgcgaattg ccgaccagtg caacgcgcga
     3781 cgccccggtg cggcgtttca gttccttgat ggcctcgcca agttcgcggc gcgcgtcttc
     3841 ggtcgatgac cggtttggct gcggtaccga atcatcggtt cgggccagcg gatcggtgaa
     3901 attgatcgca aacatccggt cgcgcggaac cccgtttgat tccatccgcc atacggtggt
     3961 gatccacagc gccgcatggt cgccattgcc gtgcacgaac agaaccggcg gaacttccgt
     4021 cgttccggga tcagctgagc tggtttgcgc agcagcgcag aacccaaagc tgttggccat
     4081 cacgggaagt atcgtggcac ctttcaggat cgtccgtcgc gatatgctca ttttcgacct
     4141 ctcggcattt tcgacatttt agccaccctg tatgaccgct gatagcggcc taaccactgg
     4201 acagccaagg actttcacgg gcatcacttg agatcgcagg gaatcaaagg ccgaccatgg
     4261 cggctgatac ggaacggacg atgacggacc agccgaaacc caagatgttt gccgccgaca
     4321 gcgtcacggc gccgctgcgg cattcggtat tccggcgtat ttggctagcg agcctgctct
     4381 ccaatttcgg gctgttgatc cagggcgtcg gcgctgcctg ggccatgacc cagatgacgt
     4441 c
//
LOCUS       DATNOI010000351         8528 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1153990,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000351 DATNOI010000000
VERSION     DATNOI010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8528
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153990"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VN968_13990"
     CDS             complement(<1..801)
                     /locus_tag="VN968_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HXL30382.1"
                     /translation="MLHDWGVIAAAFGYIGFLFFVASYGDRLSPTQRGRASALIYPLS
                     LAIYCTSWTFFGSVGFATRTSVDFLAIYIGPILMIAFGTPLLRRVIQLAKSQNITSIA
                     DFIAARYGKSQAVAATVALIAIIGSVPYIALQLKAVASSLETILSEDQAFSKIPLIGD
                     IALMVTLTMAAFAVLFGTRQTDATEHQHGLMLAIATESIVKLVAFLAAGAFVTFWMFT
                     PYELIERAMKTPEAVRAIEYVPSIGNFLTMTLLSFCAIMLLPRQFHVSV"
     gene            complement(999..2228)
                     /gene="hemA"
                     /locus_tag="VN968_13995"
     CDS             complement(999..2228)
                     /gene="hemA"
                     /locus_tag="VN968_13995"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HXL30383.1"
                     /translation="MDYSKFFSAALSRLHDERRYRVFADLERIAGRFPHAVWHSPKGP
                     CPVVIWCSNDYLGMGQHPKVVGAMVETATRVGTGAGGTRNIAGTHHPLVQLEQELADL
                     HGKQAALLFTSGYVSNQTGIATIAKLIPNCLILSDALNHNSMIEGIRQAGCERQIFRH
                     SDMADLEQLLRAAGPDRPKLIACESLYSMDGDIAPLARICDLAERYGAMTYVDEVHAV
                     GMYGPRGGGIAERDGVMHRIDILEGTLAKAFGCLGGYIAASSDIIDAVRSYAPGFIFT
                     TALPPAICSAATAAIRHLKISNWERERHQDRAARVKAILNAAGLPVMSNETHIVPLFV
                     GDPEACKRACDILLEEHHIYIQPINYPTVAKGTERLRITPSPYHDDGLIDQLAEALVQ
                     VWERLGLPLEEKSLAAE"
     gene            complement(2462..3334)
                     /locus_tag="VN968_14000"
     CDS             complement(2462..3334)
                     /locus_tag="VN968_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXL30384.1"
                     /translation="MDRQAELKIIRAAYAKQILAAARVVNDPRLEAALSAIRREDFLG
                     PGPWPIMRWFRDYVTTPDADPVYLYTDDLVGILPERQLNNGQPSLHAHLVHQASPAAG
                     EHVVHVGTGTGYYTALLAHLAGSSGRITGIEYEPELAARAKANFAGHPNVEIIEGDGT
                     LVSFDQADVIYVNAGCTRPAPNWLDRLADGGRLILPMTSDEGFGAKSIERMASAGAVF
                     RIERRGHDYLARWISGVAIFPCAGNRDEASERTLAEAFAKGGWQQVTRLYRDEEIPEE
                     RCWVSGPGWCLATS"
     gene            3429..3926
                     /locus_tag="VN968_14005"
     CDS             3429..3926
                     /locus_tag="VN968_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXL30385.1"
                     /translation="MKRQLYPAVLERGRKGAFGAWFPDFPGCVAAGRSQEETIEKAEE
                     KLAQAVDVLFEQGRPLPASTPIERIVLPKGCDLLAYFVVGVDPPDPSERVNVYLPKSL
                     IGRIDRRAAELGMSRSSFFGLAAASALARLPGPFWPEVAEPRSKVHKTGRLRTEKSSA
                     KKSSA"
     gene            complement(3937..4467)
                     /locus_tag="VN968_14010"
     CDS             complement(3937..4467)
                     /locus_tag="VN968_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6622 family protein"
                     /protein_id="HXL30386.1"
                     /translation="MLTVTRILTNTPLWVFALLAYLIWQGSQSLRPRTQPIWRMLIVP
                     LVFFLMGVSRLVTARDRGIEPLLAWLVAAVLFALLALSLRPKLLAVDRKSGTVTRPGS
                     ATPLARNVTVFVLQYAVAVATAMKLEPHAAVAIIGHAVSGASAGYFSGRAVALVRRYR
                     NFDADENSGARATDGG"
     gene            complement(4728..4991)
                     /locus_tag="VN968_14015"
     CDS             complement(4728..4991)
                     /locus_tag="VN968_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30387.1"
                     /translation="MVEKRVDRSRRKVMRAVASGVSAGLLLRISRTDASEKMTRQQAE
                     YQDTPNGLYSCGMCTLFEPPKACKVIDGEVSKDGWCKAFALAD"
     gene            complement(5016..6164)
                     /gene="solA"
                     /locus_tag="VN968_14020"
     CDS             complement(5016..6164)
                     /gene="solA"
                     /locus_tag="VN968_14020"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HXL30388.1"
                     /translation="MRSCGIAIIGLGLMGSSALYSLARRGADVLGFDPLLVGDARGSS
                     HGSCRVYRRFNFESEAYTELGDKAFEEWQTLESATGQTILKPSDVLEAGPPGAKMVAA
                     SRAAAARKGPVTGPTTGAAANAAFPAFKLPENWDVVVQKSGGILMAETAIRAFREAAR
                     GRIVRAAAQMRPTPGGIHIATSDEEVTAEQVIVAAGPWIGGFIPELGRHLRVTRQAVG
                     WFMPAKPEMVGYGEFPVFIVSGKHGLIYGFPDFEGRGVKAARHDHGPTVGADEWDPPA
                     TDDELEPVRATLAELVPGAAGPIADRDICLYTNTLKADVRVDDGDEFIIDRLPQDPRI
                     IVASPCSGHGAKFASAIGAMLADMAVDPRITAPEAFRLDRFSGFTGRP"
     gene            6218..7924
                     /locus_tag="VN968_14025"
     CDS             6218..7924
                     /locus_tag="VN968_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXL30389.1"
                     /translation="MPTLAFWMYRRIVMDASGAGSMRWLYRFALSVMLFSLMAADAYA
                     EKRVALVIGNGAYRNVPTLPNPPNDATDVAAALRRSGFDVIFETNLDQIGMQDASIRF
                     AREARTADVALFYYSGHALQFAGVNYLVPVDASLRDEADLRRMARADQILADLQQAKN
                     LRILVLDACRDNPFAEDLKRSIGRSRSANVASGLAKMESPDGTIISYATQPGRTADDG
                     SGRNSPYTSAFLKHIEDKDNVTTVFQHVSASVYEDTRGSQVPELSLSFFGEFYLNGKL
                     ENSAAPTSPASPVDPCAAAADHWRSAEAVGTVNAFDDHMLRFPTCAFAGLAKSRIAAL
                     SKPGAAVSPSNPFDGIWIVREICERKPGWQAGTYQFAGRIKDRIFHYQYGTEGKPGSA
                     TYDGKIEPDGTSEISVKGLTGDAAYDPLHRVPGTEFHFKMVIKLEGSRGAGVRSDSPR
                     PCRSEWSMLSSTAASTPNANTDSPEKSTVDGDSNPGSEKPKLRAAIAPDRREPEASRN
                     RQVTGGLSCTKMLGRCGAVCAANTGRPDCASTVCVRLQQQCLNTGCWNGRAFSGCGLI
                     KQ"
BASE COUNT         1620 a         2557 c         2747 g         1604 t
ORIGIN      
        1 cacgctgacg tgaaactggc gcggcagcag catgatcgcg cagaacgaca gcagcgtcat
       61 ggtgaggaaa ttgccgatcg agggcacata ctcgatggcg cgcaccgctt ccggcgtctt
      121 catcgcgcgt tcgatcagct cgtagggggt gaacatccag aacgtgacga aggcgccggc
      181 ggcgagaaag gccaccagct tgacgatgga ttcggtggcg atcgccagca tcaggccgtg
      241 ctggtgttcg gtggcgtcgg tctggcgggt cccgaacagc accgcgaatg ccgccatcgt
      301 cagcgtgacc atcagcgcga tatcgccgat cagcggaatc ttggaaaagg cctggtcttc
      361 gctgaggatg gtttccagcg aggaggccac cgccttgagc tgcagcgcga tatacggcac
      421 cgatccgatg atcgcgatca gcgcgacggt tgccgcgacc gcctggctct tgccgtagcg
      481 tgccgcgatg aaatcggcga tcgaggtgat gttctgcgat ttggcgagct ggatcacgcg
      541 gcgcagcagc ggcgtgccaa atgcgatcat caggatcggg ccgatataga tcgcgagaaa
      601 atccacgctg gtgcgggtgg cgaatccgac cgagccgaaa aacgtccacg acgtgcaata
      661 gatcgccagc gacagcggat agatcagcgc gctggcgcgc ccgcgctgcg tcggcgaaag
      721 gcggtcgcca tagctcgcga cgaaaaacag gaacccgatg tagccgaagg cggctgcgat
      781 cacgccccag tcatgcagca tcgccgctta cctcccggga tcacgatccg atgagacgga
      841 tcacgggtca acgcgaacca ccgccgacca gggaaaggag tatagccgga tcaagccggc
      901 aaagcatccg gtgtcgccga cctggatccg ggattcgccc gggaggagtt aacagcatcg
      961 ggttaacgct atgggcgttg acgccgatga ggttaacgct attcggccgc cagggacttt
     1021 tcctcaagcg gcaggccgag acgctcccag acctgcacca gtgcttcggc gagttgatcg
     1081 atcagtccgt cgtcgtgata gggcgaaggc gtgatccgca gccgctcggt gcctttggcg
     1141 acggtcggat agttgatcgg ctggatgtag atgtggtgtt cttccagcag gatatcgcag
     1201 gcccgcttgc aggcttcggg gtcgccgacg aacagcggca cgatgtgggt ctcgttcgac
     1261 atcaccggca gccccgcggc attgaggatc gccttgaccc gggcggcgcg gtcctggtgg
     1321 cgttcgcgct cccagttcga gatcttgagg tgccggatcg cggcggtcgc cgccgaacag
     1381 atcgcgggcg gcagcgcggt ggtgaagata aagccgggcg cgtaggaacg caccgcatcg
     1441 atgatgtctg aagatgctgc gatatagccg ccgaggcagc cgaacgcctt ggccagcgtg
     1501 ccttccagaa tatcgatccg gtgcatgacg ccgtcgcgct cggcgatgcc gccgccgcgc
     1561 ggaccataca tgccgacggc gtgaacctcg tcgacatagg tcatcgcgcc gtagcgctcg
     1621 gcgagatcgc agattcttgc cagcggcgcg atgtcgccgt ccatcgaata caggctctcg
     1681 caggcgatca gtttcggccg gtcggggccg gcggcgcgca agagttgttc cagatccgcc
     1741 atgtcgctgt ggcggaagat ctggcgttcg cagccggcct ggcggatgcc ttcgatcatc
     1801 gaattgtgat tgagcgcatc cgacaggatc aggcaattcg gaatgagctt ggcgatggtc
     1861 gcgatgccgg tctggttcga gacatagccc gaagtgaaca gcagcgcggc ctgcttgcca
     1921 tgcagatcgg ccagttcctg ttcgagctgc accaggggat ggtgggtgcc cgcgatgttg
     1981 cgggtgccgc cggcgccggt gccgacccgc gtggcggtct cgaccatggc gccgaccacc
     2041 tttgggtgct gccccatgcc gagatagtcg ttggagcacc agatcacgac cgggcacggt
     2101 cccttcgggg aatgccacac cgcatgcgga aaccggcccg cgatccgctc gagatcggcg
     2161 aaaacccggt agcgccgctc gtcatgcagg cggctgaggg cggcactgaa gaatttgctg
     2221 taatccatcg caagacccga agcggagcgt gaggccggga gcggcgaagc ctttttagag
     2281 cgtttccagg tttgatgtcg agccggaatt ggctttttcg gtcgcgctat ggttggcaga
     2341 gggttgcatc ctgcgcgcac aaaaatttga tctggatcaa gacgttggat cgactttgct
     2401 gtccgcggta ttttggaaaa agtgaggcgg ttttgcagcg aacctcgcca ccgctcgaac
     2461 gctagctggt ggcgaggcac catccgggtc cgctgaccca gcagcgctct tcgggaattt
     2521 cttcgtcgcg atagagccgc gtgacctgct gccagcctcc tttggcaaac gcttcggcca
     2581 gcgtgcgttc ggatgcctcg tcgcggtttc cggcgcaggg aaagatcgcc acgcccgaaa
     2641 tccatcgcgc caaatagtca tgaccgcgcc gctcgattcg aaacaccgcg ccggcacttg
     2701 ccattcgctc gatcgacttt gccccaaaac cctcgtccga cgtcatcggc aggatcaaac
     2761 gcccgccatc ggcgagacga tcgagccagt ttggggctgg ccgcgtgcag ccggcgttga
     2821 cgtagatgac atcggcctga tcgaacgaca ccaaagtgcc gtcgccctcg atgatttcga
     2881 cgtttgggtg gcccgcgaaa ttggctttgg cgcgcgcggc gagttcaggc tcatattcta
     2941 ttccggtaat cctgcctgag gatcccgcga ggtgagccag gagggcggtg taataaccgg
     3001 tgccggttcc gacatgcacg acgtgttcgc ccgccgccgg cgatgcctgg tgaacgaggt
     3061 gcgcatgcaa cgacggttgc ccgttgttga gctgccgttc cggcagaatg ccgacgagat
     3121 cgtcggtgta gagataaacc ggatcggcgt cgggcgtcgt gacgtaatcc cggaaccagc
     3181 gcatgatcgg ccacggaccc ggcccgagaa aatcctcgcg gcggatggcg ctaagcgcgg
     3241 cctcgagacg cggatcgttg acgacgcgcg cggcggccag aatctgtttg gcgtaggccg
     3301 cccggatgat tttgagttcg gcttggcggt ccatgagact ctttcgccct atcgaggctg
     3361 gtgctagggt ggtgtgggat taaacacacc gatgtgtgtg aaatagcaca agctcgcgga
     3421 gcggccaaat gaaaagacag ctttatccgg cggttctgga gcgcggtcgg aagggggcct
     3481 tcggcgcctg gtttcccgat ttcccgggct gcgtcgccgc cgggagatcg caggaggaaa
     3541 ccatcgaaaa ggcggaggag aagctggcgc aggcggtgga tgtgctgttc gaacagggca
     3601 ggccgttgcc ggcgtcgacc ccgatcgagc ggatcgtgct gcccaagggc tgcgacctgc
     3661 ttgcctattt tgtcgtgggg gtggacccgc cggatccgtc ggagcgcgtg aacgtgtacc
     3721 ttccgaagag cctgatcggc cggatcgacc ggcgcgcggc ggaactgggc atgagccgct
     3781 ccagcttttt cgggctcgcc gccgcctcgg cgttggcccg gctcccgggc ccgttttggc
     3841 cggaggtggc ggagccccgc tcgaaggtgc acaagaccgg caggctgcga actgaaaaat
     3901 cctcggccaa aaaatcgtcg gcgtaacggg gcggttctac ccgccgtcgg tcgcgcgtgc
     3961 gccggaattt tcgtccgcgt cgaaattcct gtaacggcgc accagcgcca ccgcccggcc
     4021 cgaaaaataa cccgcgctgg caccggagac ggcgtggccg atgatcgcca cggcggcgtg
     4081 gggttcgagc ttcatggcgg tcgccaccgc gaccgcgtat tgcagcacga acaccgtgac
     4141 gttgcgcgcc agaggggttg cgctgcccgg acgggtcacg gtgccgcttt ttctgtccac
     4201 ggcgagcagt tttggcctaa gggaaagcgc cagcaaggca aacaagactg ccgccaccag
     4261 ccacgcgaga agcggctcga tccccctgtc gcgcgccgtg accagccgcg acacgcccat
     4321 cagaaaaaac accaagggca cgatcagcat tcgccagatc ggctgcgttc tcggccgcag
     4381 cgattgcgag ccttgccaga tcaggtaggc cagcaacgca aaaacccaga gcggggtatt
     4441 ggtgaggatg cgggtgaccg tcagcatcgg ctctcctgat ggcgtgttgt ccggcccgtt
     4501 gtgaagcgaa acacggatat gtcatatcgc ggcgctcatg cgggctgctt gtcggcatcg
     4561 aggtaggcgg cgccaagcgc agcccggatg agctaacggg cgcgcgaatg cgcgcccgat
     4621 cataaactcc gcgacatctg ggattttcat atttcctttg aaccccgcag atcgctgtgc
     4681 tcatgcgagc tacttgttcc tctaacgggc tggttgttcc tccaagttta atccgcgagc
     4741 gcgaaggctt tgcaccagcc gtccttgctg acctcaccgt cgatcacctt gcaggcttta
     4801 ggtggctcga acaacgtgca cattccgcaa ctgtaaaggc cgtttggcgt gtcctgatat
     4861 tcagcttgct gccgcgtcat tttttctgat gcgtcggtcc gcgaaatacg caacaaaagc
     4921 cctgccgaga caccgctggc aaccgcgcgc atgacctttc gccgcgacct gtctacccgt
     4981 ttttcgacca tgcaggttcc tccccttcag caatttcatg gccgaccggt gaatccggag
     5041 aacctgtcca gccgaaaggc ttcgggcgcc gtgatccgtg ggtcgaccgc catgtccgcc
     5101 agcatggcac cgatcgcgct ggcgaacttc gcgccgtgac cggagcaagg cgaagcaacg
     5161 atgatccggg ggtcctgagg cagccggtcg atgatgaact cgtcgccgtc gtccacgcgc
     5221 acatcggctt tcagcgtgtt ggtgtagagg cagatatcgc gatcagcgat cggcccggcc
     5281 gcaccgggca cgagttcggc aagtgtcgcc ctgaccggct ccagttcgtc atcggtggcc
     5341 ggtggatccc attcgtcggc gccgacggtg gggccatgat cgtgccgcgc ggctttgacg
     5401 ccgcggcctt cgaagtcggg aaatccgtat atcaggccgt gcttgccgct gacgataaac
     5461 accggaaact cgccataacc caccatttcg ggtttggccg gcataaacca gccgaccgcc
     5521 tgccgggtca cccgaaggtg gcgtccgagt tccgggatga agcctccgat ccacggaccc
     5581 gcggcgacga taacctgttc ggccgtcacc tcttcgtccg atgtcgcgat gtggatgccg
     5641 cccggcgtcg gtcgcatctg cgcggcggct cggacgattc gacccctggc cgcttcgcgg
     5701 aacgcgcgaa tggccgtctc ggccatcagg atgccgccgc ttttctgaac gacgacatcc
     5761 cagttctcag ggagtttgaa ggcgggaaac gctgcattgg ccgcggcgcc ggtggtcggg
     5821 ccggtgacgg ggcccttccg cgctgcggcg gcgcgggacg ctgcgaccat ctttgcgccg
     5881 ggcggaccgg cttcgagcac atcgcttggc ttgagaatgg tctggccggt tgcggactct
     5941 agcgtctgcc attcctcaaa ggccttgtcg ccgagttcgg tgtaggcttc gctctcgaag
     6001 ttgaagcggc ggtagacccg gcatgagccg tgcgaagagc cgcgtgcatc gccgaccaga
     6061 aggggatcga acccgagcac gtcggccccg cggcgcgcca gcgagtagag cgcggagctt
     6121 cccataagac ccaagccgat gatcgcgatt ccacagctgc gcatggcgaa gctcttatcg
     6181 tttatggacg atgccgccaa gcctggcggc ttccccaatg ccgacccttg cattttggat
     6241 gtaccgacgg atagtgatgg atgcatcagg agctgggtcc atgcgctggc tgtatcgttt
     6301 tgccttaagc gtcatgctgt tttcgctcat ggcggcagat gcctatgccg agaagcgggt
     6361 cgcgctggtg atcggcaacg gtgcctatag gaacgttccc accttgccca atccgccgaa
     6421 cgacgccacg gacgtggccg ccgcactcag gaggtcgggt tttgatgtca tctttgagac
     6481 gaacctcgac cagatcggca tgcaggatgc ctccatccgc tttgcgcggg aggcgcgcac
     6541 cgcggacgtt gcattgtttt attacagcgg gcacgccctg cagtttgcag gcgtcaatta
     6601 tctggtgccc gtcgacgcca gtcttcgaga tgaggcggat ctcagacgga tggcgagggc
     6661 agatcagatt ctcgccgatc tgcaacaagc aaagaatttg aggatcctcg tgctcgacgc
     6721 ttgtcgagac aatccgtttg cggaggatct caaacgctcc atcgggcgaa gccgaagcgc
     6781 caatgtcgca agcggtctcg ccaagatgga aagccctgat ggaacgatca tatcctatgc
     6841 cactcaaccc gggcgaacgg cagacgacgg gtccggccgc aacagccctt atacgagcgc
     6901 gttcttgaag cacattgagg acaaggacaa cgtcaccacc gtgttccagc atgtcagtgc
     6961 cagcgtatat gaggacacca ggggatcgca ggttcccgag ttgtcgttat cgttcttcgg
     7021 cgagttttat ttgaacggca aactggagaa tagcgcggcg ccgacatcgc ccgcatcgcc
     7081 cgtcgacccc tgcgccgctg ccgccgatca ttggcggagc gccgaagctg tcgggactgt
     7141 gaatgcattt gacgatcaca tgcttcgttt tccaacctgc gcattcgcag gacttgccaa
     7201 atccagaatt gcagcacttt cgaagcccgg cgcggccgta tcgccgtcga accctttcga
     7261 cggaatttgg atcgtcagag aaatctgtga aaggaagccg ggctggcaag ccggtacgta
     7321 ccagttcgcg ggccggataa aggaccgcat ctttcattac caatatggca cggaaggaaa
     7381 accggggtcc gcgacctacg atggaaagat cgagccagat ggcacctccg agatctcggt
     7441 gaagggcctc acgggcgacg cggcctatga tccgcttcac cgtgttccgg gaacggaatt
     7501 ccacttcaag atggtgatca agctggaggg gtcacgtggc gccggtgttc gcagcgattc
     7561 tccgcgcccc tgtcgttccg aatggtcgat gctttcttcg accgctgctt caactccaaa
     7621 cgccaatacg gactcgccgg aaaaatcaac cgttgacgga gattcaaacc ccggatcgga
     7681 aaagccgaag ctccgcgcgg cgatcgcacc ggatcgccgc gagccggagg cgtcccgaaa
     7741 tcgacaagtg acgggtggtc tgagttgcac caagatgttg ggaagatgcg gcgccgtttg
     7801 cgctgctaat acgggtcgtc cggattgcgc ctcgacggta tgcgtgcgcc tgcaacagca
     7861 atgcttgaac accggctgct ggaacgggcg cgcattcagc ggctgcggac tgatcaaaca
     7921 atagttggtt cgcctgatgc ggccggtttt ggcgaagcgt aaccgcgcga caatgcggcg
     7981 caatacattt cgctgttgcg ctcaggcgcc gttggccccg tccgggcaac gtagctcgcc
     8041 tttcgggctt gcgcgcaacg gcgaatttat tcaaccggat caagctgatg ctagccgtcc
     8101 agtccccttt gcaaaaatat tttagttttc acacacccca aatcacttct agaactttgc
     8161 gcatcccgtc ccacacagag gggcgtttcg cgatcgtcac ggacgtaggg cacgggatgc
     8221 ggtggacgcg gcagcgtttt gcgcgcgatg ggatcgcagg gcgggttctt agggactttg
     8281 tgagcgatca acagcgcgct gacgaaagat gctttcgcgg acggcaaaat cgtgtggtcc
     8341 tgacgccccg acgctggcgt caagttttgc ggaaggcaag tcggcccaac cgggcgcgga
     8401 caagtcagat atccgctgac gacggtggca aaagagcccg gtcaccgggg agagcgcgac
     8461 ataagccgta aaaccattgc gtgcgggaat gccgggtgat gccggtgtac tcgttgttac
     8521 tcgtgtgc
//
LOCUS       DATNOI010000352         9128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_717461,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000352 DATNOI010000000
VERSION     DATNOI010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9128
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717461"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VN968_14030"
     CDS             <1..345
                     /locus_tag="VN968_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXL30390.1"
                     /translation="LNAPIRDDGEAGEWQDWLVDTSPNQEAIMAEHEEFDHRREALNG
                     AIGVLNPRERRIFEARRLAEDPMTLEDLAAEFGVSRERVRQIEVRAFEKVQSAVKGTI
                     ARQEQAALEAAH"
     gene            complement(436..945)
                     /locus_tag="VN968_14035"
     CDS             complement(436..945)
                     /locus_tag="VN968_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30391.1"
                     /translation="MSASIIRPVAAGHRHLFRVHGIMALRLFPAVVPLLYASVLAASE
                     TPSKPFLEKNSFYLSSAGFRVQVANNPAGQKALRTLPAHRFVVNGVGDAVRYLYAEPQ
                     HCVCIFVGTQQAYDRYRQILSQPLRPTDNVPPDYKTQAGMLLSNQPLRQSTRGDPTTL
                     SDYLSTLHY"
     gene            complement(1134..2438)
                     /locus_tag="VN968_14040"
     CDS             complement(1134..2438)
                     /locus_tag="VN968_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL30392.1"
                     /translation="MTHSLRGRLLIGLTLMIVSTGIGAGAVGFQWAFDEAIEMQDAIL
                     TQVGALALNTRFQNDASTSSGVNAEARVTIEELGDRPSGVPDARSLWILQDGLHVVAR
                     DQKPWRFLLRTRPDGSRFAVGQPTEIRDEIARDSAFHTILPFAALAPCLMLVIAIVVW
                     QSLRPMTELAGQLDVRRSDDLSRLPLKGTPRELHPFIASINRLLERIQTMMDQQRRFV
                     ADAAHELRTPITAISLQAENLSQVELAAESRDRLIALKNGARRTAHLLEQLLALARHD
                     MNSPPEDLVTSLDRCAKEVVSDFMPTAMDRGVDLGFEIIEPSCVRGEAAALSSVVHNL
                     IDNALRHTPRGGRVDIGIYHEGARVILQIEDTGTGIPAAELERVFEPFVRGSLPAEEG
                     TGLGLSIVKRIVERLEGSVTLENLPKSGLCVIVSFPDAGEGA"
     gene            complement(2435..3097)
                     /locus_tag="VN968_14045"
     CDS             complement(2435..3097)
                     /locus_tag="VN968_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL30393.1"
                     /translation="MRVLLVEDDRMVGAAVEQALKDAAYAVDWVTDGRTAIEAAQAES
                     YDVALLDLGLPEADGQEVLRRLRQTGRQLPVIIVTARDGIDDRIDGLDLGADDYLVKP
                     FEIRELLARMRAVLRRQGSGSSSILGNGKISLDPATREATFAGETSLLTAREFAVLQA
                     LLMRPGAILSRGDLERHIYGWNEEVESNAIEFLIHTIRKKLGALAILNVRGVGWMVDR
                     AL"
     gene            complement(3165..3494)
                     /locus_tag="VN968_14050"
     CDS             complement(3165..3494)
                     /locus_tag="VN968_14050"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HXL30394.1"
                     /translation="MHRSTFMHCTAAIFLSASALAGTTTLSTKHAVAVPYAANSLAAA
                     VAAAEKRLKGQAIGVDHEQQADGRLVYEIEVRNGSNVFDVEVDADKGTVIATAEGRPG
                     ADDDKSD"
     gene            3743..4501
                     /locus_tag="VN968_14055"
     CDS             3743..4501
                     /locus_tag="VN968_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30395.1"
                     /translation="MLNKVPQVTLSFWVIKIMSTTVGETGADYLAVHVGLGSAITSAV
                     TVSLLLAALFMQLRMRRYVPWIYWLTVVLVSVVGTQITDALTDGLGVSLYASTSAFAV
                     ALAATFAVWYRSERTLSIHTIVTTRRELFYWAAILFTFALGTAAGDLATEALGLGFNV
                     GVVVFGALIAAIASAYYLGANAVLTFWLAYILTRPLGASLGDLLSQARTYGGFGLGTA
                     YTSVAFLIVIVALVTFVSFDGRRLHHADNAGETP"
     gene            4570..4965
                     /locus_tag="VN968_14060"
     CDS             4570..4965
                     /locus_tag="VN968_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXL30396.1"
                     /translation="MKSRKLILVLSVLGALAGPAGVGSLVAASIVTSAEAAMSSKLGD
                     LSSFRTIVTDTTALVDKGDLAGAKTRIKDLETSWDEAEPGLKPRAAAEWHKVDKAIDR
                     ALEALRATAPNAANCKQALSDLLAVMDGA"
     gene            5052..5807
                     /locus_tag="VN968_14065"
     CDS             5052..5807
                     /locus_tag="VN968_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003596877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30397.1"
                     /translation="MNGISDRSSKVPAVTLAFWVVKILATTLGETGGDAVTMSMNLGY
                     LVGTTIFTAVLVVAISVQIASKRFHPFLYWTVIVATTTTGTTLADFVDRSLGIGYLGG
                     STLLAILLMLSLGLWYRSMGSISTDSITSTKAEIFYWATIMFSQTLGTALGDWMADST
                     GLGYRGGAVVFAIGLAVIAAAYYWTTISRITLFWAAFVLTRPLGATVGDFLDKPLDHG
                     GLALSRYIASVALAVLIVALIATLPQRAGRHPA"
     gene            5820..6167
                     /locus_tag="VN968_14070"
     CDS             5820..6167
                     /locus_tag="VN968_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30398.1"
                     /translation="MSIILQSTVVGFSAEFIRRLPELEQAMVAHGLELSQFVISKDRA
                     TPTTLPVIGPFYDYTVFAGDEIFTVTEPDDIKFLEYFYQRCIAEDATPPAPQGRRRLG
                     LISRLFSWMTQPI"
     gene            complement(6592..7215)
                     /locus_tag="VN968_14075"
     CDS             complement(6592..7215)
                     /locus_tag="VN968_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HXL30399.1"
                     /translation="MNFRSLVTALCVVAYLTPASAQTPDLTMIARLSGTPDIPGLKLV
                     WLAPWGEVSKAHPWRNIIVHQTEGPAGSARGGAQQQAKNPTRRGVTVWVETDGTVFWA
                     VPENLVPTHGDGANRNDNKYIDNGPTFHQVIGSNSIGVEFAGNYPDVTRGATEAQVAA
                     WRILVAVLRARYDIPLERVYAHNWIDYKDARYCEGCALATLARQWGE"
     gene            complement(7253..8476)
                     /locus_tag="VN968_14080"
     CDS             complement(7253..8476)
                     /locus_tag="VN968_14080"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXL30400.1"
                     /translation="MLSVPTLWTAYVVNFLALGLVWAYVTRSYPKFEAARFWTGSAFI
                     AAAGAALAMLRMFLDSLLPLLAAATILIFAACLAAMGVKRFYDRPVSWRGTALISGLS
                     FAGMTFFLVAYDSTAMRILIYSLGQCLPFALTLRLLLSRQHGRSSPGARLAATVATLI
                     IALYVIRVAANLLPVGAGFSFVQFSPLQSALVLVLMFLSMVWNFGCLLMAMDRLRNEV
                     ADLALLDDLTGVGNRRHLLQRLTEECARSERSGEPFALLVVDLDGFKAINDTHGHAAG
                     DACLQHFTLMAQTRLRPSDMLARSGGDEFCIVLPASTLCEGAMIARRILDVCREDAEA
                     CVGADIPIALSIGVAQWTREIGMFPDRLMAAADHALYAAKKDGKNCHAVYDPAPLSPE
                     ITAQAMPNPALRQHA"
     gene            complement(8689..8892)
                     /locus_tag="VN968_14085"
     CDS             complement(8689..8892)
                     /locus_tag="VN968_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30401.1"
                     /translation="MRCHTGVIALSTAVMHFDTLFLKFLVRRVQAKREDPGIHQSAAT
                     MDCRIKSGDDAMQEHHRTIHDAA"
BASE COUNT         1739 a         2857 c         2827 g         1705 t
ORIGIN      
        1 ctcaacgcac cgatccgcga cgacggcgag gccggcgaat ggcaggactg gctggtcgat
       61 acctcgccca accaggaagc catcatggcc gagcacgagg agttcgacca tcgccgcgag
      121 gcgctgaacg gcgccatcgg cgtgctcaac ccccgggaac gccgcatctt cgaggcgcgg
      181 cgcctggccg aagatccgat gacactggaa gacctcgccg ccgaattcgg cgtgtcgcgc
      241 gaacgcgtgc ggcagatcga ggtccgcgcc ttcgaaaaag tgcagtcggc cgtgaagggc
      301 acgattgcgc ggcaggaaca ggccgcactg gaagccgcgc attaatctgc gcggcatcaa
      361 aacgagacac gaaagccggc ggcgacgccg gcttttttgt tgacgctgtc gcgggtggcg
      421 gtcccgggcg gcctgtcaat aatgaagcgt gctgagatag tccgacagcg tggtgggatc
      481 gccgcgcgtg ctctgccgca gcggctggtt gctcaacagc atgccggcct gggtcttgta
      541 atcaggaggc acattgtcgg tcggcctcag cggctggctg aggatctggc ggtacctgtc
      601 ataggcctgt tgggtgccga cgaagatgca gacgcaatgc tgcggttcgg cataaagata
      661 acgcacggcg tcgccaaccc cgttgacgac gaagcggtgc gccggcagcg tgcgcagcgc
      721 tttttgcccg gccggattgt tggcgacctg gacacgaaaa ccggcggacg acagataaaa
      781 gctgttcttt tccaggaacg gcttgctcgg cgtctccgag gcggccagaa ccgaggcgta
      841 gagcaacggc acaacggccg gaaacaggcg caaagccatg atgccatgga cccggaacag
      901 atgccgatgg cccgctgcga ccggccggat gatcgatgct gacattgcaa attccccctg
      961 cctgagcgtt gcctgttgcg ccggctataa cgccgcgaac agtgcgccaa gattgtgttg
     1021 cacctcgagc ggcatccagc cgaccaagaa tagtgggggg catatgcgac ccaatcccac
     1081 gcaggcagca accgcatagg gggcaaaaag ccggcggcaa cgccggcttg cttctatgcc
     1141 ccctcacccg catcgggaaa ggaaacgatc acgcaaaggc cggactttgg caggttctcc
     1201 agggtcaccg agccttcgag ccgttcgact atgcgcttga cgatcgaaag gccgaggccg
     1261 gttccctcct cggcaggcag gcttccgcga acgaaaggtt cgaatacccg ttccaattcc
     1321 gccgccggaa tgccggtgcc ggtgtcctcg atttgcaaaa tgacccttgc gccttcgtgg
     1381 tagattccga tatcgacccg gccccctcgc ggcgtgtggc gcagcgcgtt gtcgatcaga
     1441 ttgtggacca cggacgagag cgccgccgcc tcgccgcgca cgcatgaggg ctcgatgatt
     1501 tcaaacccga gatcgacgcc gcggtccatc gccgtgggca tgaagtccga aaccacttcc
     1561 ttggcgcagc gatccagcga tgtgacgaga tcctccggcg ggctgttcat gtcgtgccgc
     1621 gccagcgcga gcaattgctc cagcaggtga gccgtcctgc gggcgccgtt tttcagcgcg
     1681 atcagccggt cacggctctc ggcggcaagt tccacctgac ttagattctc cgcctgaaga
     1741 ctgatcgcgg tgatgggcgt ccgaagttcg tgggcggcat cggcgacgaa acgccgctgc
     1801 tggtccatca tcgtctgtat tcgttcgagc agccggttga tcgaagcgat gaacggatgg
     1861 agctcgcgcg gggtgccctt caggggaagt ctgctcagat cgtccgagcg cctgacgtcc
     1921 agctgtccgg cgagttcagt catcggtcgc agcgattgcc atacgacgat cgcgatcacc
     1981 agcatcaggc acggcgccag ggctgcgaat ggcagaatcg tatggaaggc gctgtcgcgc
     2041 gcgatctcgt cgcgtatctc ggtgggttgg ccgacggcaa accggcttcc gtccggccgt
     2101 gtccgcagca ggaaacgcca cggcttctga tcgcgggcga cgacatgcaa accgtcctga
     2161 aggatccaga gcgagcgagc gtcgggcacg ccgctcggcc tgtcgcccag ttcctcgatc
     2221 gttacccgcg cctccgcgtt gacgccgctg ctggtcgacg catcgttctg gaagcgggta
     2281 ttgagcgcca gggcgccgac ctgggtaagg atcgcgtcct gcatctcgat cgcttcatcg
     2341 aaggcccact ggaaacccac cgcacctgcg ccgatacccg tcgagacgat catgagcgta
     2401 agtccgatca ggagacgtcc gcgcagggag tgggtcataa ggcgcggtcc accatccagc
     2461 cgacgccgcg gacattgagg atcgcgaggg ctccgagctt cttgcgtatc gtatggatca
     2521 ggaattcgat cgcgttgctc tcgacttctt cattccatcc atagatgtgg cgttcgagat
     2581 cgccgcgcga cagaatcgca cccgggcgca tcaggagcgc ctgaagaacc gcaaactcgc
     2641 gcgcagtaag cagcgacgtc tcgccggcga atgtagcctc ccttgtcgcc ggatcgaggc
     2701 ttatttttcc gttgccgagt attgaagacg agccgctgcc ctgacgccgg agaacggcgc
     2761 gcatccgcgc cagcagctcg cggatctcga agggtttcac cagatagtcg tccgctccga
     2821 ggtcgaggcc atcgatacgg tcgtcgatgc cgtcgcgggc cgtcacgata atcaccggaa
     2881 gctgacggcc ggtctggcga agccggcgca ggacttcctg gccatcggct tcgggcaggc
     2941 caagatcgag aagcgcgacg tcgtagctct cggcttgcgc ggcttcgatc gccgtccggc
     3001 catcggtgac ccaatcgacc gcataggcgg cgtccttcag cgcctgctcc acggcggccc
     3061 cgaccatacg atcatcttcg acgagcagaa cgcgcatggt ttatcctgtc ggcacatccg
     3121 ccgagcgcaa tgggcccgcc atgatcgcgt cggtttgaaa gttgctaatc ggatttgtcg
     3181 tcatcggcac cgggcctgcc ctcggccgtt gcaatgactg tacccttgtc ggcatcgact
     3241 tccacatcga aaacgtttga cccgttgcgg acctcgattt catagaccaa ccggccgtct
     3301 gcctgctgct catgatcgac gccgatggcc tgtcctttaa gccgtttctc cgcagcggca
     3361 acggcggcag ccagcgaatt ggccgcatag ggaacggcga ccgcatgctt cgttgagagc
     3421 gtggtggttc cggcgagagc gcttgccgat agaaaaatgg cggccgtgca gtgcatgaac
     3481 gtagatcggt gcatgtcgtg gttcccctga cgtctattcg aacctgaatt ctgtcactcc
     3541 gaaacttagg gccgacttag cggtctgcgc gcaccgtgaa cgttgaacgg ccggacacga
     3601 aaacgaccct tccttctaag tcgccgctaa gtgacgaccg gcaaggtttt gcgtagcggt
     3661 acgccggtct tcgaagccaa ttcgagccca ttgatgaagg gatatccgtg tgcgcgatcc
     3721 tgtgaatcaa cgctcctgcc aaatgctcaa caaggttccg caagtcacgc tttcgttctg
     3781 ggtcatcaag atcatgtcga ccaccgtggg tgagaccggt gcggattatc tggcggtgca
     3841 tgtcggacta gggtccgcga tcaccagtgc cgtcaccgtg tcgctgctgc tcgcggcgtt
     3901 gtttatgcaa ttgcggatgc ggcgctatgt gccgtggatc tattggttga ccgtcgtgct
     3961 ggtgagtgtc gtcggaaccc agattaccga cgcgctcacc gatgggcttg gcgtcagcct
     4021 gtatgccagc acttcggcat tcgcggtggc tttggccgcg acattcgcgg tctggtaccg
     4081 cagcgaacgc acgctttcca ttcacaccat cgtcaccacc cggcgtgaac tgttttactg
     4141 ggcggccatt ctttttacgt ttgcgctggg caccgcggcc ggcgacctgg ccaccgaagc
     4201 gcttggtctc ggtttcaacg ttggcgtcgt ggtgtttggc gcgctcatcg ccgcgatcgc
     4261 ttcggcctat tatttgggcg cgaacgccgt gctcactttt tggctcgcct atatcctgac
     4321 aaggccgctg ggtgcctcgc ttggcgatct gttgtcccag gcgcgcacct atggcgggtt
     4381 cggtctcggc accgcttaca ccagcgtcgc cttcctcatc gtcatcgtcg cactagtgac
     4441 gttcgtcagt ttcgatggac gacgcctgca tcacgccgat aacgcgggcg aaacgccata
     4501 atgcaaatcg ttcgatttca ccacgcgctt gcacctgtcg tgcgagccga tcagcacagg
     4561 agacaagtca tgaaaagccg gaaactgatt ttggtcttga gcgtgttggg cgcccttgcc
     4621 ggacccgccg gcgtcgggtc gttggtagcc gcgtccatcg ttacgtcagc cgaagctgcg
     4681 atgtcatcga aactcggtga cctctcgtcg ttccggacca tcgtcacgga taccaccgcg
     4741 ctcgtcgaca agggcgatct cgccggcgcg aagacccgca tcaaggatct ggaaacgtcg
     4801 tgggacgagg ccgagccggg attgaagccg agggcggctg ccgagtggca caaggtcgac
     4861 aaggcgatcg accgcgcgct ggaggccttg cgcgccaccg ccccgaatgc cgccaattgc
     4921 aagcaagccc tcagcgatct gctcgcggtc atggacgggg cttgaaagca cggatagcgc
     4981 ccggccgaga gaaccaacgc gacgtcgcaa ccatccaccc ggcactgtcg ggctcaatcg
     5041 ggatacaaac catgaatggc atttccgacc gctccagcaa ggtccccgcg gtcacactcg
     5101 cattttgggt cgtaaaaatc cttgcgacca cgttgggcga gaccgggggc gacgcggtca
     5161 ccatgtccat gaacctcgga tatctggtcg gaacgaccat cttcacagcc gttctcgtcg
     5221 tcgcgatcag cgtccagatc gcatcgaagc gcttccatcc gtttctgtat tggaccgtca
     5281 tcgtcgccac gacgaccaca ggcaccacgc ttgccgattt cgtcgaccgc tcgcttggga
     5341 tcggctacct cggaggttcc acgctcctcg cgatcttgct gatgttgtcg cttggcctct
     5401 ggtaccgttc gatgggttcg atctcaaccg actccatcac gtccacgaaa gccgagatat
     5461 tctattgggc cacgatcatg ttctcgcaaa cgctcggcac cgcgcttgga gactggatgg
     5521 ccgattccac cggccttgga taccgtggcg gagccgtggt ttttgcgatc ggtctcgccg
     5581 tcatagccgc cgcctattac tggaccacga tctcgcgcat caccctgttc tgggcggcct
     5641 tcgtgctcac tcggccactg ggtgcaaccg tcggtgattt cctggacaag ccgctggatc
     5701 acggcggact ggcgctcagc cggtatattg cgtcagtggc gctggcagtg ctgatcgtcg
     5761 ctttgattgc aacgctgccg cagcgggctg gccgacatcc ggcatgacag ggcggcgctg
     5821 tgtcgatcat tctgcaatcc accgtcgtcg ggttttccgc cgaattcatc cgcaggctgc
     5881 cggagcttga gcaggcgatg gtcgcacatg ggctggagct gtcgcaattc gtgatctcga
     5941 aggaccgtgc gacgccaacc accttgccgg tgatcgggcc gttctacgac tacaccgtat
     6001 tcgccggcga cgagattttc accgtcaccg agccggacga catcaaattc ctggagtatt
     6061 tttaccagcg ctgcatcgcg gaggacgcga ccccgccggc accgcaaggc agacgaaggc
     6121 tcggcctgat cagccggctc ttcagctgga tgacgcagcc gatttgaggg agatcgtgtc
     6181 ccggacgcgg tgcagcgtcc gtcacgcagc tccgcaaggc ggccagaacc gaggcgtaga
     6241 gcaaccgcca gctccggaag gccggcgtgc cccacgtctg gagccaccga acgacatcgt
     6301 cattcgccgc gaccgaacct tgcgcctcaa acgctttcca gcggcgatca agctcaacga
     6361 tgtcatcgac gtcggcgtcg gcaggacccg cctcaagcgt gaccaattcc gccaccagtt
     6421 ctccggcacg ctcaggccgc gttccgcggc gcgttgcttg agcgccattg cagtgtcatt
     6481 atcgatttcg ataatctggt cttcatttga ggatcataac cgaacacgcg tcccgaatcc
     6541 agaaagctgt cgcacatcat ccggggcacg atagctgtcc gcggagcgac cttactctcc
     6601 ccattgcctt gccagcgtcg ccagcgcgca gccttcgcaa tagcgcgcgt ccttgtagtc
     6661 gatccaattg tgggcataga cccgctcgag gggaatgtcg tagcgcgccc gcagcacggc
     6721 taccagaatc cgccacgccg cgacctgcgc ctcggtcgca ccgcgcgtca cgtcgggata
     6781 attgccggcg aactccaccc cgatcgaatt actgccgatc acctgatgaa aggtcgggcc
     6841 attgtcgatg tatttgttgt cgttgcggtt ggcgccgtcg ccatgggtcg gaaccagatt
     6901 ttccggcact gcccaaaaca ccgtgccgtc ggtttcgacc cagaccgtga cgccgcgccg
     6961 ggtcgggttt ttcgcctgct gctgcgcgcc gccgcgtgca gacccggccg gcccctcggt
     7021 ctggtgcacg ataatgttgc gccagggatg ggcttttgac acctcgcccc acggcgccag
     7081 ccagaccagt ttcagacctg gaatatccgg tgtgcccgac aaacgagcga tcatggtaag
     7141 gtccggcgtt tgcgcggagg ccggagtgag gtatgcgaca acgcagagcg ccgtcaccag
     7201 cgagcggaag ttcatgagtc ctgggatcca atcagtagcg tgattagcac gctcacgcgt
     7261 gctggcgcag ggccggattc ggcatcgcct gggcggtgat ttcaggcgac agcggcgccg
     7321 gatcatagac ggcatggcag ttcttgccat ccttcttggc ggcataaagc gcatggtctg
     7381 cggcggccat cagccggtcc gggaacatgc cgatctcccg ggtccattgc gcaacgccga
     7441 tcgacagcgc gatcgggata tccgctccga cacaggcttc ggcgtcctcg cggcacacat
     7501 ccaggatgcg gcgcgcgatc attgcgccct cgcacagcgt cgaagcgggc agcacgatgc
     7561 agaattcgtc gccacctgag cgcgccagca tgtcgctggg cctcaagcgc gtctgcgcca
     7621 tcagggtgaa atgctgcagg caggcgtcgc cggcggcatg gccatgggtg tcgttgatcg
     7681 ccttgaagcc gtcgaggtcg accaccagca gtgcaaacgg ctcaccgctg cgctcggacc
     7741 tggcgcattc ctcggtcagg cgctgcaaca gatggcggcg attgccgacg ccggtgagat
     7801 cgtcgagcag cgcgagatca gcgacctcgt tgcgcagccg gtccatcgcc atcagcaggc
     7861 agccgaaatt ccacaccatc gacaggaaca tcagcaccag gaccagggcc gactgcaacg
     7921 ggctgaactg gacgaaggaa aacccggcgc cgacaggcag caggttggct gccacgcgga
     7981 tgacgtagag ggcgataatg agagtggcga ccgtggctgc gagccgcgcg ccggggctgc
     8041 tacggccatg ctgccgcgac agcaacagcc tgagtgtcag cgcgaacggc agacactggc
     8101 ccagtgaata gatcagtatc cgcatcgccg tgctgtcgta ggcgacgagg aagaacgtca
     8161 tgccggcgaa gcttagtccc gatatcagcg cggtgccgcg ccaggagacc ggccggtcgt
     8221 agaaccgctt gacacccatc gcggcaaggc aggcggcgaa tatcaggatg gtggcggcgg
     8281 ccaggagcgg cagcagcgaa tccaagaaca tgcgcagcat cgccagcgcc gcgccggcag
     8341 ccgcgatgaa tgccgaaccg gtccagaacc gtgctgcctc gaatttcgga tagctgcgcg
     8401 tgacataggc ccagaccagg ccgagcgcca gaaaattgac gacgtacgcc gtccacagcg
     8461 tcgggacgct cagcatggcg aattcagaac gcgcacatcc agtcccctgt cgtcgaacag
     8521 cccggttagg acaaccctat ttttcgaggc gtagcatgcg gcgatgtcgc ttaacgggct
     8581 ctgatccgcg acggcgcact tcgcagtgtg gttttgaatt gatgaagacg gcgcgcgccg
     8641 gttccggatc gatgcagaat gcgccggcgc gttggtacct cgttaactct aagcggcgtc
     8701 gtgaatggtg cgatgatgct cttgcatcgc gtcatcgccg gacttgatcc ggcaatccat
     8761 cgtggctgca gattgatgga tgcccgggtc ttcacgctta gcctgaacgc gccgcacgag
     8821 aaatttcaga aaaagcgtat caaaatgcat aacagctgtg gataacgcga tgacaccggt
     8881 gtgacagcgc aaggcagcac gccgcgaatc tgttcggatg atgagtgagg ctgttgcgag
     8941 gctagccctc cccgagcatg cctcgtgtcg cgtttccttc cattaaactc aaaagaggat
     9001 gctatggcta agaaagcgaa gaaggcgaaa aaggcgaaaa aggcaaaaaa ggccaagaag
     9061 gccaagaagt aaccttcaag cccgggtggg gcctcgctcc gcccgggcac ccgtcgccgg
     9121 gtccaaca
//
LOCUS       DATNOI010000353         2399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_836895,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000353 DATNOI010000000
VERSION     DATNOI010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836895"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VN968_14090"
     CDS             <1..640
                     /locus_tag="VN968_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXL30402.1"
                     /translation="SLRKTIAQQVPDLIVLDLNMPEEDGLSLIRDLKRRTSVPIIMLT
                     ATASAIDRVVGLELGADDYLAKPCELRELLARIRSVLRRSSAVQPVAQPAAAVPQPAP
                     ASTRVRFGTKWLDHDAQALRDDEGNEHPLTASEYGLLKVFAANPKRVLSRDRLLELAN
                     ARDAEAFDRAIDLRIMRIRRKIEPDPARPSVIRTVRGGGYLFSPTGEAPALD"
     gene            820..1428
                     /locus_tag="VN968_14095"
     CDS             820..1428
                     /locus_tag="VN968_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXL30403.1"
                     /translation="MQNQYVLRDVASPHFKAPAQDTSDEALIDLIADGDKRAMQVLYA
                     RHNVRVYRFILRLTGNQSLAEDLVSEVFLDVWRQAEAFESKSQVSTWLLAIARYKALS
                     ALRRRTDEHLDDHMAATIEDTADSPETMVHNKDRSIIVQKCLTQLSPAHREVIDLVYY
                     HEKSVDEVARIVGVPAATVKTRMFYARSKMADLLKQSGVHAF"
     gene            complement(1587..1886)
                     /locus_tag="VN968_14100"
     CDS             complement(1587..1886)
                     /locus_tag="VN968_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30404.1"
                     /translation="MTKRSGADIEQPRVEPEIIPPSDVRSRRAGGSFDGTQRVYVARV
                     GPFGFAMVALAIAALAMLLFLLVLGAFVILIPLAGLLLAVIIAVGLFRVLIWRRP"
     gene            1885..>2399
                     /locus_tag="VN968_14105"
     CDS             1885..>2399
                     /locus_tag="VN968_14105"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXL30405.1"
                     /translation="MIASRWCPGRMQCRDRGRRPQREGSKEHCNMTDALREKLVQAGR
                     VLVSEDQGDYVAGHVTLRLPDGDGRFLMKPAGIGLEEMTPDNIITVDREGERVGGTMP
                     RHSEVFIHSEVLRVRSDIAAVVHTHPPHAVAFSSLGRPLLPVGNDGAIFCAGVPVFSE
                     TTDLIIDQARGR"
BASE COUNT          463 a          779 c          746 g          411 t
ORIGIN      
        1 cagcctgcgc aagaccatcg cgcagcaggt gccggacctg atcgtcctcg atctcaatat
       61 gccggaggag gacggtcttt cgctcatccg cgatctcaaa cggcgcacca gcgtgccgat
      121 catcatgctc accgcaaccg cgagcgcgat cgatcgcgtg gtcggcctgg aactcggcgc
      181 ggacgactat ctggccaagc cgtgcgagct gcgcgagctc ttggcccgca tccgctcggt
      241 gctgcgccgg agcagcgcgg ttcaaccggt agcgcaacct gcggcggcag tcccgcagcc
      301 agcgcccgca agcacgcggg tgcggtttgg aacgaagtgg ctcgaccacg acgcccaggc
      361 gctgcgcgac gacgagggta acgagcatcc cctgacggcg tcggaatacg gcttgctcaa
      421 ggtcttcgcc gccaacccca agcgggtgct ctcgcgcgat cgcctgctgg aactggccaa
      481 tgcccgcgat gcggaggcct ttgatcgggc gatcgacctg cgaatcatgc ggatccggcg
      541 gaaaatcgag cccgatccgg cgcggccctc ggtgatccgg accgttcgcg gcggcggata
      601 tctgttttct ccgaccgggg aggcaccggc gctggactga gacaaaattg tctcatcaga
      661 tgaaacaatt gatggtatca aattaccttt atttcgtcgc cgcgtcagcc acgaaacaat
      721 tgagcctgcg acgaaaccat tttccaggtt ccacgtaaca tcgaagcaac aggcctctgc
      781 tagtcaactg ggttaagggc tgattaatag gggatcgtca tgcaaaatca gtatgtgttg
      841 cgcgacgtcg cgtcgccgca cttcaaggct cctgcgcaag acacgtcgga cgaggcgctg
      901 atcgacctca tcgccgacgg cgacaagcgg gccatgcaag tgctctacgc ccggcataac
      961 gttcgggtct accgcttcat ccttcgcctg acgggcaacc agtcgctggc cgaggacctc
     1021 gtgagcgagg tgttcctcga cgtctggcgg caggccgagg cgttcgaatc aaaatcccag
     1081 gtgtccacct ggttgctcgc gatcgcgcgc tacaaggcgc tctccgccct gcggcgccgc
     1141 accgacgagc acttggacga tcacatggcg gcgaccatcg aggataccgc cgatagtcct
     1201 gagaccatgg tgcacaacaa ggaccgtagc atcatcgtcc agaagtgcct gacgcagctc
     1261 tcgccggcgc accgcgaagt catcgacctc gtttactatc acgagaagtc ggtcgacgaa
     1321 gtcgcgcgga tcgtcggggt gccggcagcg acagtgaaga cccgcatgtt ctatgcgcgg
     1381 agcaagatgg ccgaccttct caagcagtcg ggggtgcacg cattctgatc gcggcgtccg
     1441 cgttccctcg gacgattacc ctcgcgctta atgtccatga tcaggcccgg cgggctggca
     1501 cctcaggtgt cagcccgccg ataatttttg ggctgtgggc gtccggcata ttccgctcgg
     1561 gatgaccgcc cacccacgcg gccgggtcag ggccgccgcc aaatcaagac ccggaacagc
     1621 ccgaccgcaa tgatgaccgc aagcagcaag cccgccagcg gtatcaagat cacgaacgcg
     1681 cccagcacca gcaggaaaag aagcatcgcc agcgccgcga tcgcgagcgc caccatcgcg
     1741 aaaccgaacg ggccgaccct cgcgacatag actcgctgcg tgccgtcgaa tgacccgccg
     1801 gctcggcgcg aacgcacgtc gctgggcggg atgatctccg gttcgacacg gggttgctcg
     1861 atgtctgctc cggatcgctt ggtcatgatc gcctcacgct ggtgccctgg tcggatgcaa
     1921 tgtagggatc gcggcagacg gccacaacgg gagggctcga aagagcattg caacatgacc
     1981 gatgctttgc gcgaaaaact cgtccaagcg ggccgcgtgc tcgtgagcga ggaccaaggt
     2041 gattacgtcg caggacacgt gacattgcgt ctgccggacg gcgacgggcg ctttttgatg
     2101 aagcccgccg gcatcggtct cgaggagatg acgcccgaca atatcatcac cgttgatcgc
     2161 gagggcgagc gcgtcggcgg cactatgccg cgtcatagcg aggtcttcat ccattccgaa
     2221 gtcttgcgcg tgcgatccga cattgccgcc gtcgtccaca cccatccgcc gcacgcggtg
     2281 gcgttctcat cgctcggccg gccgttgctg ccggtgggaa acgacggcgc catcttctgc
     2341 gcgggcgtgc cggtgttctc ggagacgacc gacctcatca tcgaccaagc gcgcggccg
//
LOCUS       DATNOI010000354        22366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_310670,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000354 DATNOI010000000
VERSION     DATNOI010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 22366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22366
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310670"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="VN968_14110"
     CDS             complement(<1..1469)
                     /locus_tag="VN968_14110"
                     /inference="COORDINATES: protein motif:HMM:NF004829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HXL30406.1"
                     /translation="MSGTSSYDVAIVGYGPVGAVFANLLARYGLRIAVVERAADIYDK
                     PRAITLDHEALRVFQAVGLAEFMESAIAPHNGSHYLGVDGELIKMFDPMPPPYPLGWI
                     PNATFVQPDAEKALRGKFAEYGNADVFLSTTGVSLAQDDTTVSLRVRPESGDDFTLTA
                     RYLVGCDGANSFVRKHLGIGLEDLAFDEWWMVVDTLTSEPGKRPARSYQYCWPSRPGT
                     FVPGPGKLRRWEIKLLPGEDPELAGAPDNVEKLLKGFTDTSDLTIWRSAVYRFHALLG
                     DSWRAHRVFLMGDATHQTPPFLGQGLCAGIRDASNLAWKLALVLRGNAGDALLDSYEV
                     ERKPHVRSVVASAKEFGKIIGELDPVAAAARDQRLRAELKSGKAETIRQKFIPDLASG
                     LIASRNKLAGRLFVQPRVRAVDGNERRLDDLLKPEFAVVTATQEAMSWMSEASLSCWH
                     QLGGERVVIAEAGKSTMQDGIATLVETDGVFSNWMRENRV"
     gene            complement(1466..2164)
                     /locus_tag="VN968_14115"
     CDS             complement(1466..2164)
                     /locus_tag="VN968_14115"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXL30407.1"
                     /translation="MPEIALDSLETTKETLASQVYDRLRQDIISVAIEPGEKLHIRSL
                     CARFDVGLSPMREALSRLSSEGLVAQSDHRGFAVAPMGEQDLVDLTRARCWLNELAIR
                     KSIAHGDAAWEEQVVLSFHRLSRTPRFEPGKEQERSPKWEIAHRNFHNGLISASGSRR
                     LEQYCDHLFDSSERYRHVGRKAGIKGENREMEHRALMEAVVARDGDTAARLITAHFER
                     TAELVREVLRGRAR"
     gene            complement(2174..3178)
                     /locus_tag="VN968_14120"
     CDS             complement(2174..3178)
                     /locus_tag="VN968_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007796138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL30408.1"
                     /translation="MILPSTNFDPGFRITRASHVVLAVRDLAASRQFYERVIGLILTA
                     EEDDTLYFRGVEEACHHSLVLRKGSDICCARLGLRVFSEQDLDKLKGFFEARGCPAAW
                     ADVAHQGRTLHAIDPAGTLLEFCATMPVMPRMITKFTRHAGGSALRLDHYQILTPDVR
                     KVCEFYTAAGFRLSEYIAPPGTFDLRGVFLQRKGNPHDIVFFNGAGPRLHHFAFLAPE
                     VHDLLYACDLAGELGLGASVERGPGRHGPGHAMYVYMRDPDGHRVELFNTHYQIMDIE
                     NEPIAWDPTDHNIAFPWGLPARQAWFEQATPFADVAIREPQEKPNPMTLEKYLASQNA
                     "
     gene            complement(3208..4512)
                     /locus_tag="VN968_14125"
     CDS             complement(3208..4512)
                     /locus_tag="VN968_14125"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXL30409.1"
                     /translation="MTVTGIGIAGIVALFALVALHIPLAFAMIVVGIVAFALQTEWAS
                     ALTFLATEPSQVLGSMDLAAVPLFLMMGAFVNVSGFSRDLYAAAAALLGHRRGGLSYA
                     TIGGSAAFGAICGSSPATVATFTQVALPEMLKRNYAPGFSGATIAAGGALKALIPPSL
                     SMILYCVVARTYIFDVFVAAVIPALITIAANLTAIAVVVRLNPAIAPVSPRVSRKEKL
                     AALWRAAPAALLIAIIFAGLYSGVFTVNEAASVAAVVSFVFALIRRTITPSNLASGLH
                     QTALIAGMIYFVLIGSSIFTYFISLAKVPEQLIGFLGGVNLPHTGIIVLMLAAYLVLG
                     AVFDELAAMIVTLPFALPIIVYFGYDPVWWAIINVIIVELGLVIPPIGLVILIINAMR
                     PDIPLGTLYRGIVPFVAADLAVLGLLTAFPSLALWLPAILRG"
     gene            complement(4509..5018)
                     /locus_tag="VN968_14130"
     CDS             complement(4509..5018)
                     /locus_tag="VN968_14130"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXL30410.1"
                     /translation="MRAARTIVTGLANISAVAGLSLLLILAVFTLLDGLLRAIANTPI
                     DLVREIGDMIAAICGACCLPIVLLHRNNIVLRLFDRILPPWGVRGIDTLAALVVEIVM
                     IGMAWQFGLFGMKTMRAGDVTWLLNWPKAPFWFVVDGVLWVAVAVQTFVLIEEVLGVR
                     ERREPESVA"
     gene            complement(5015..6025)
                     /gene="dctP"
                     /locus_tag="VN968_14135"
     CDS             complement(5015..6025)
                     /gene="dctP"
                     /locus_tag="VN968_14135"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXL30411.1"
                     /translation="MTRLCKALLALFAVWAPLPATAEPVTLKLSFFASETEVNYARAI
                     KPWVDAVNADPSRAVRIEAYPNGALGKALPAQPQLILDGVADIAFVNPSLGPGRFPDD
                     QVFELPGLLQNLTEGVKLYEELVKANLLHGYSDYYVVGAFMNPQYMIFARRPIKSIAD
                     LKGMKVRINGAVIGQTVKELGMVPVLMPPNEVVEAMGRGTIDATTTVPAAVVDFGIDR
                     VTSHDYLLPLGAGPLAVLMNRKKFDSLPQPAQDVIAKYSIRWIDDTYVRELGKYNDEL
                     IARFKADPKRTVTAPSQGDIAALQPVYQKVNAEWVAKSPGNAELLGKVRELLARIRAN
                     QP"
     gene            6219..6998
                     /locus_tag="VN968_14140"
     CDS             6219..6998
                     /locus_tag="VN968_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL30412.1"
                     /translation="MNLGIEGRTAIVCASSRGLGLACAQALAEEGVHVLINGRDQAKV
                     DKTAAALREVARGDVIAIAADVTTTDGQAKLLAACPSPDILVNNNAGPAPGNFADIER
                     DRWLKAIEANMIAPLMLIRAVLPAMKQRRFGRIVNITSAMVTTPRPHMTVSSGARAGL
                     TAAIKGLSLEVAPYNVTINNLLPERFDTDRQRQMAKAAMARENISYEEARARQIESIA
                     AKRLGDPKEFGATCAFLCSTLAGFMSGQNLHLDGGSYPALI"
     gene            7620..7994
                     /locus_tag="VN968_14145"
     CDS             7620..7994
                     /locus_tag="VN968_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30413.1"
                     /translation="MAKASAKKRTLLRAKQRRAASKGNSVLIKTFKRELVTSGLVVSK
                     ARFEAVMLAAEQSGLLSEKGSRIGGRVSPALVRQAKRQTGIETDTDLIEFALATVALE
                     DNFAEAFKESRGKVDPTVKLGF"
     gene            7996..8562
                     /locus_tag="VN968_14150"
     CDS             7996..8562
                     /locus_tag="VN968_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HXL30414.1"
                     /translation="MAEFDFDAARRWARFDPENTLARRSDDELPFVSASLIAGQGLLL
                     DTCVYIDQMQDRSPPVLDDLIAQRQVNHSTVAIQELMHTVGVLKPSDVRTAPVVDVIR
                     NQIKAMPPHRIFAPDIEVLGRAALLSGILCRLQAYGKDGKLRALQDCVLFLQAQKLGF
                     VVLTANVGDYDILLQLIPNGRVLFYRSK"
     gene            complement(8794..8878)
                     /locus_tag="VN968_14155"
     tRNA            complement(8794..8878)
                     /locus_tag="VN968_14155"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8842..8844),aa:Leu,seq:gag)
     gene            complement(9010..9252)
                     /locus_tag="VN968_14160"
     CDS             complement(9010..9252)
                     /locus_tag="VN968_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HXL30415.1"
                     /translation="MPTLDKVTVDLMVVLGTTSMPIHQVMRLSRGAIIELDATEADEV
                     KILANNLPIASGVVLVDRNRIAVEVKQMLPRLPGSR"
     gene            9321..10046
                     /gene="lipB"
                     /locus_tag="VN968_14165"
     CDS             9321..10046
                     /gene="lipB"
                     /locus_tag="VN968_14165"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HXL30416.1"
                     /translation="MVNDRQSLDLTTLARPADGGAVEWQVSRAPVPYLDAVAAMEVRV
                     AAIAAGEAPELVWLLEHPPLYTSGTSGNARDLLDARFPLFATGRGGQLTYHGPGQRVA
                     YVMLDLKRRRPDVRAYVASLEEWIIRTLDAFGVHGERREDRVGVWVQRPDKGSGCEDK
                     VAALGVRLRRWVSFHGISINVAPDLTHFDAIVPCGVVDPRYGVTSLADLGHPATMADV
                     DIALRRAFEAVFGAVRTRLPEPI"
     gene            complement(10071..10922)
                     /locus_tag="VN968_14170"
     CDS             complement(10071..10922)
                     /locus_tag="VN968_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXL30417.1"
                     /translation="MTATSRMLLVLALLFGFGQTRAQTPSVAVSGVDAALIEDIVIGS
                     RVLADFGVLDGFGHVSARHPTNPNHFLMSRSLAPALVTADDIMEFDLDGNAVGSSGRS
                     VFLERFIHSEIYKARPDVMSVVHTHSPGVVPFTVSQVPLRPMYHNAAFLAAGAPVWDI
                     RRDFGETDMLVRDPTLGKSLAQTLGDRSVVLMRGHGDVTVGPAVKVAVFRAYYTDVNA
                     RLQSQAVALGGDITYLTPGEGAKADAINLLVLDRIWNLWKMRVAPSLGQSLGQSLGST
                     LGPSLGK"
     gene            complement(11029..12468)
                     /locus_tag="VN968_14175"
     CDS             complement(11029..12468)
                     /locus_tag="VN968_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HXL30418.1"
                     /translation="MTSPFLWTLIAIQIAMGAFDTFYHHELTERLAWRPSQRHELQLH
                     SVRNALYALLFLTLGWLEVHGFWAMIVIAVLVIEVVVTLMDFVEEDLSRKLPASERIN
                     HTLLALNYGGILVLLLPILIEWAGQPAAIKPAFHGFWSILATVSAVGAGLGGARDFAA
                     SRRLARLNIATVSGLARELPGRQSILVTGATGFIGSRLVLALADAGHQVIALVRNPAK
                     AETLGPPITLITSLDQLPADARIDAIVNLAGEPIGNGLWTEAKRRKILDSRIDMTGAL
                     VQLIARLEGRPAVLVSGSAIGWYGLWQDQPLTESAKAHGCFSHELCDAWEKAASRAAE
                     YGVRVVHVRIGLVVGTEGGLLTRMLTPFEFGLGGPLGSGQQWMSWIERDDLVRLIVHA
                     LAKSDISGPINATAPIPVRNMKFSEELGRRLHRPAVFRVPAALLHRLAGDFADELLLG
                     GQRVIPNKALSSGFVFRHETLRSAFEAIL"
     gene            complement(12465..13124)
                     /locus_tag="VN968_14180"
     CDS             complement(12465..13124)
                     /locus_tag="VN968_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HXL30419.1"
                     /translation="MPKLRAPAASDESLDGNLSDLRFRALLPDEDWGRLPLATWRRFS
                     KRLADGKTAIYVGEVVEVRFSRIGWWLAQLARLIGGPLPTGGDTHVPSIVTVTEDAAT
                     GGQVWTRIYARRNGFPQVIHSSKRFSGPTGLEEYVGFGVSMALRMLVDGRALQFRSAG
                     YFVQVGRLRLPLPALLTPGALTVTHSDCGSGEFRFTLEIIHPRFGTLIRQSAVFREAT
                     P"
     gene            complement(13167..13724)
                     /locus_tag="VN968_14185"
     CDS             complement(13167..13724)
                     /locus_tag="VN968_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXL30420.1"
                     /translation="MTEITAKKKLPAAVERFVLHWGDMGDEWGVNRSVSQIHGLLYLS
                     ETPMTADDIAETLGMARSNVSNSIRELLSWNLIRRVPIMGDRRDHYEAETDIWEVAAR
                     IAAGRKEREIDPAVDALRACVVDAADDPTISPVASKRLKEMLAFTELVDRWYAQMLTV
                     PRPRLIALIKLGEKIVSFLPAGKSK"
     gene            13983..14243
                     /locus_tag="VN968_14190"
     CDS             13983..14243
                     /locus_tag="VN968_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30421.1"
                     /translation="MIALLAGPAYAQQKSVPKYGDVTPDKSPNEIEQDRAAERAYKRS
                     LGNIPDQGPTDPWGNARSVDAPKAVAKPSAAKPRTKTGASAN"
     gene            complement(14293..14592)
                     /locus_tag="VN968_14195"
     CDS             complement(14293..14592)
                     /locus_tag="VN968_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HXL30422.1"
                     /translation="MGGTIRQVTIRGRVQGVGYRYWVEHQAVAHGLEGWVRNRRDGSV
                     EALFAGPEDVVSNMVASCRRGPSSARVDAIDQHPGDADALNLRQPGERFSVLPTI"
     gene            complement(14593..15459)
                     /locus_tag="VN968_14200"
     CDS             complement(14593..15459)
                     /locus_tag="VN968_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HXL30423.1"
                     /translation="MAFRKRREALRSILAGSTCIRPGSVYDAISIRVAEDLGFEVGMF
                     GGSVASLAVLGDPDIALITLTELAEQMRRMSRAAALPVMVDADHGYGNALNVRRTVQE
                     LETAGAAGLTIEDTLLPQAFGQAKTQLISLEEGVGKMKAALDGRGDPSLVIMARTGAA
                     SVSGLEDAIGRARAYEAAGVDALFFTGVKNRAELEAIAAATTLPIVLGGFPEEMIDPD
                     YLAGKRVRIALQGHAPIAAATLAVYETLKALCDGQSPKNLKGLASSDLTGRVTRDADM
                     KVRAAEFLGLKR"
     gene            complement(15530..17545)
                     /locus_tag="VN968_14205"
     CDS             complement(15530..17545)
                     /locus_tag="VN968_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HXL30424.1"
                     /translation="MFKRILIANRGEIACRVIKTARRMGIETVAVYSEADRDALHVEM
                     ADKAVLIGPPAAAESYLVMEKIVEACRKTGAQAVHPGYGFLSEREAFPRVLSEAGIVF
                     VGPNAGAIAAMGDKIESKKAAAKADVSTVPGYLGIITDERHAVKIADGIGYPVMIKAS
                     AGGGGKGMRIAHSTSEVAEGFGLAKAEAKASFGDDRVFIEKFIIDPRHIEIQVLGDKH
                     GNVIYLGERECSIQRRNQKVIEEAPSPLLDETTRRRMGEQAVALAKAVNYDSAGTVEF
                     VAGQDKSFYFLEMNTRLQVEHPVTELITGIDLVEQMIRVAAGEKLALKQKDVKLSGWA
                     VESRVYAEDPFRNFLPSIGRLVKYRPPVESSIDGITVRNDTGVQEGGEISIYYDPMIA
                     KLVTHASSRAAAIEAQATALDSFYIDGIRHNIPFLSALMLHPRWREGNLSTGFIAEEF
                     PRGFAVRVPEGEVARRLAAVAAAIDHVLGERKRQISGQLIHRAVQRERRRAVWLDRNE
                     IALDIAREDDFITVRFVGAASNPGHPHQLVSPWVPGDPVWQGSVDGHPVAMQVRPIPN
                     GFRLAHQGFEVAVNVFTESEAAAARLMPVKTATDTGKKLLCPMPGLVVSIAVAEGQEV
                     KSGETLAVVEAMKMQNVLRAERDGTVKKIHATAGATLAVDALILEFV"
     gene            18012..19856
                     /locus_tag="VN968_14210"
     CDS             18012..19856
                     /locus_tag="VN968_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXL30425.1"
                     /translation="MRRSILLLSAGMLVLSAGMASAQNSTPHNLILFVPDGLRALKVT
                     PETAPAMAEVRDKGVNFKNPHSLFPTFTMANGSAMATGHHLGDTGTFSNTIYTGFSSA
                     PAGDTVVPFIENDAVLGDVDEHFHGDYLNEETILKMARAKGFSTAAIGKVGPTLIFDH
                     TDRADRPGLHSIVIDDATGSKNGVPLSEEMKAMLTKANLPLAAPSRGDNGKAGDAKTP
                     GTMVPNTAQQAYFADVASQIVLPLFKERAKPFVLVFWSRDPDGSQHNTGDSLNTITPG
                     INGPTSLAGIKNADNNLAQLRKALDDLGLSATTNVMIAADHGFSTISKESKTSPSAKA
                     TYEDTPKDFLPMGFLAIDLAKALDLPLFDPNDKNARIAENAHPKAGNGVLGQDPAKPD
                     LVVATNGGSDLIYLPKKDKKLAGRAVKALLEQDYVSGIFVDDALGRYPGALPLSALNL
                     KGKAVTPQPSIVVNFRSYASGCDEPTTCAVEVADTVLRQGQGMHGSFSRGDTMNFMAA
                     TGPDFKAGYVDALPVSNADVGMTIAQLLDLHAAAVGGLTGRVMSEALPNGIVSKFADG
                     TISSKPDPNGLQTVVKFQRVLSQRYFDVAGFPGRTVGLEAGKQKTAGK"
     gene            complement(20782..21357)
                     /locus_tag="VN968_14215"
     CDS             complement(20782..21357)
                     /locus_tag="VN968_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30426.1"
                     /translation="MSASAGMPAEPALEPSEISCRDSAALALTVVRDGVITGEGPTTK
                     GRVHFSRALDADDAAWCARILTATAVEHEPVSRAEAEALFEINDAAAERSDDGRFDDL
                     LAKAVVHHAASESGLPVPSRTVALSPDTAIESWAPTRTAGVDTEVLEWIASQMRGKHR
                     SNRTLMRMVATIVGAAALPFSQLPNVFDIGM"
     gene            21357..21620
                     /locus_tag="VN968_14220"
     CDS             21357..21620
                     /locus_tag="VN968_14220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30427.1"
                     /translation="MEFSFVFFQSQQFSMPGVNEWLTIRNLRADSCKQLLIGRCDVRH
                     GPRGAANWPGNGAVRKRQNRDNDAFEDIVIFPSQWGVLKDAAN"
     gene            21735..21953
                     /locus_tag="VN968_14225"
     CDS             21735..21953
                     /locus_tag="VN968_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30428.1"
                     /translation="MKAQLARFVIFQIAMLVALVIFMTMDVGWLSAVLIVISVIAGLS
                     LSDLAFHRLADPETIQRDLEERVRNQHL"
     gene            22005..>22366
                     /locus_tag="VN968_14230"
     CDS             22005..>22366
                     /locus_tag="VN968_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXL30429.1"
                     /translation="MALQIGAVAPDFEAETTEGKINFHDWIGNSWALLFSHPKDFTPV
                     CTTELGALAKLKPEFDKRGVKLMGLSVDPVDKHAKWSEDIKETQGAAPNYPMIGDTDF
                     NVSKLYDMLPASTSGDVAT"
BASE COUNT         4542 a         6951 c         6759 g         4114 t
ORIGIN      
        1 acccggttct ctcgcatcca gttcgaaaat accccgtcgg tttcgaccaa ggtcgcgatt
       61 ccgtcttgca tcgtgctttt cccggcttcg gcgataacca cgcgttcgcc gccaagctgg
      121 tgccagcagg aaagcgaagc ctccgacatc cacgacatcg cctcctgcgt tgctgtgact
      181 accgcaaatt ccggcttcag caggtcgtca agacgcctct cgttcccgtc cactgcccgc
      241 acccgcggct gcacgaataa cctgcccgcg agcttgttgc gagaggcgat cagtccggaa
      301 gccagatcgg gaatgaattt ctgccgtatc gtttcggctt ttccggattt gagttccgca
      361 cgcaaccgct gatcgcgagc agcggcggct accgggtcca attccccgat tatcttgccg
      421 aattccttgg cgctggcgac gaccgagcgg acatgcggtt tgcgttcgac ctcgtagctg
      481 tcgagcaagg catcaccggc gttgccgcgc agaacaagtg ccagtttcca ggcgagattg
      541 gaagcatcgc gaatgcctgc acagaggccc tgtcccagga atggtggcgt ctggtgcgtg
      601 gcgtccccca tcaggaacac ccgatgggct cgccagctgt cgccgagcaa ggcgtgaaag
      661 cgatagaccg ccgagcgcca gatggtcaga tcggacgtat cggtgaaacc cttgagcaac
      721 ttttccacat tgtcaggcgc accggcgagc tccgggtctt cgccgggcaa tagcttgatc
      781 tcccaacgcc gcaattttcc cggcccgggt acgaacgtcc ccgggcgcga aggccagcaa
      841 tattgatagc tcctggcggg tctcttgcca ggttcgctcg tgagggtatc caccaccatc
      901 caccattcgt cgaaggcgag atcctccagg ccaatgccga ggtgttttcg gacgaaactg
      961 ttggcgccgt cgcatccgac gagatagcgc gcggtcagcg tgaagtcgtc gccggattcc
     1021 ggcctcacgc gcagcgatac cgtcgtgtca tcctgcgcca gcgacacgcc ggtcgtcgac
     1081 agaaagacgt cggcattgcc gtattcggca aatttgccgc gcaacgcctt ctcggcatcg
     1141 ggctgaacga aggtcgcatt cggaatccag ccgagcggat atggcggcgg catcggatcg
     1201 aacatcttga tgagctcgcc gtcgaccccg agataatgcg agccgttatg cggcgcgatc
     1261 gcactctcca tgaactccgc gagaccaacg gcctggaaga cccgcagcgc ttcgtgatcg
     1321 agcgtgatcg cgcgcggctt gtcgtaaata tcggcggcgc gctcgacgac ggcgatcctg
     1381 agaccgtacc tcgccaacag gttggcgaag acggcgccga ccggtccgta gccgacgatc
     1441 gccacgtcgt agcttgaggt cccgctcaac gcgcgcgccc tctgagcacc tcgcggacga
     1501 gctcggcggt gcgctcgaaa tgcgccgtga tgagtctcgc cgccgtatcc ccgtcacgcg
     1561 cgaccaccgc ctccatcagc gcgcgatgtt ccatctcgcg attctcgccc ttgatgcctg
     1621 ccttgcggcc gacgtgccgg tagcgctcgg acgaatcgaa cagatgatcg cagtattgct
     1681 cgagccgccg cgaacccgag gccgagatca ggccattatg aaaattgcga tgcgcaatct
     1741 cccatttcgg gctgcgctct tgctccttgc cgggctcaaa gcgcggcgtg cgcgacaagc
     1801 gatggaatga gagcacgact tgctcctccc atgcagcatc gccgtgagcg atggacttcc
     1861 ggatcgcaag ttcattgagc cagcatcggg cgcgcgtgag gtcgacgaga tcctgctcac
     1921 ccatcggcgc gaccgcgaag ccgcgatgat cgctctgcgc gaccagcccc tcgctcgaca
     1981 ggcggctcaa cgcctcgcgc atcgggctta acccgacatc aaaacgtgcg cagagcgagc
     2041 ggatatgcag tttctcgccg ggttcaatgg caaccgagat gatatcctgt cgcagccggt
     2101 catagacctg cgaggccagc gtctccttgg tggtttccag actgtcgagc gcgatttcgg
     2161 gcatggattg ccttcaggca ttctggctgg cgagatattt ctcaagcgtc atcgggtttg
     2221 gcttctcttg cggctcgcgt atcgcgacat ccgcgaaggg cgtcgcctgc tcgaaccagg
     2281 cctggcgcgc cggtaatccc cagggaaaag cgatgttgtg gtcggttggg tcccaggcga
     2341 tcggctcatt ctcgatatcc atgatctgat agtgggtgtt gaacagctcg acccggtgac
     2401 catccggatc gcgcatgtag acatacatcg catgccccgg cccgtgccgg ccgggaccgc
     2461 gctcgaccga cgcgcccaga ccgagttcgc ccgcgagatc gcaggcatac aatagatcgt
     2521 gcacctccgg tgccaggaag gcgaagtgat gcaggcgcgg gccggcgccg ttgaagaaga
     2581 cgatgtcatg cggattgccc ttgcgctgca gaaacacgcc ccgcaaatcg aacgtgcccg
     2641 gcggggcgat atattcgctc agccggaagc ctgcggcggt gtaaaactcg cacaccttgc
     2701 ggacgtcggg cgtcaggatc tgatagtggt cgagccgcag cgccgagccg ccagcatggc
     2761 gggtgaactt cgtgatcatg cgcggcatca ccggcatggt ggcgcagaat tccagcagcg
     2821 ttccggccgg atcgatcgca tgcagcgttc gaccctgatg cgcgacgtct gcccacgccg
     2881 ccgggcagcc gcgcgcctca aagaatccct tgagcttgtc gaggtcctgc tcggagaaca
     2941 cacgcaagcc gaggcgcgcg cagcagatat cactcccttt acgcagtaca aggctgtggt
     3001 gacaggcctc ctcgacgccg cggaagtaga gcgtgtcgtc ttcttccgca gtcaggatca
     3061 ggcctatcac cctctcgtag aattgccggc tcgccgccag gtcgcggacg gcaagaacga
     3121 catggcttgc ccgcgtaatg cgaaatcccg gatcgaagtt cgtgcttgga aggatcatgc
     3181 tccagccttt cgtttcgtca tttgtaatca accacgcagg atggccggca gccacagagc
     3241 cagtgacggg aatgccgtca acagcccgag aaccgcaaga tcggccgcca cgaacggaac
     3301 gataccgcgg tagagcgtac cgagcgggat atccggccgc atcgcattga tgatcaggat
     3361 caccaggccg atcggcggaa tcaccagccc caactcgacg atgatgacgt tgatgatcgc
     3421 ccaccacacc gggtcatagc cgaaatagac gatgatcggc agtgcgaacg gcaaggtgac
     3481 gatcatcgcc gcgagttcgt cgaacacggc gccgagcacc agataggcgg caagcatcag
     3541 cacgatgatg ccggtgtgcg gcagattgac gccgccgaga aacccgatca gctgttcggg
     3601 gacttttgcc aggctgatga aataggtgaa gatcgaggag ccgatcagca cgaagtagat
     3661 catgccggca atcaatgcgg tttggtgcag gccgctagcg agattgctcg gtgtgatcgt
     3721 acggcggatc agcgcgaaca caaacgacac caccgcagcc accgaggcgg cctcgttaac
     3781 ggtgaagacg ccgctataga gtccggcgaa gatgatcgcg atcaacagag cggcgggcgc
     3841 cgcgcgccat aatgcggcga gcttctcctt tcgtgagacg cgcggggaga ccggcgcaat
     3901 cgcggggttg aggcgcacca ccactgcgat cgcggtcaaa ttggctgcga tcgtgatcag
     3961 ggcgggaatt acggcggcga cgaagacgtc gaaaatatag gtccgcgcca cgacgcaata
     4021 gaggatcatg ctgagcgagg gcgggatcag cgccttcagc gcaccaccgg ctgcgatcgt
     4081 cgctccggaa aaacccggcg cgtagttgcg cttgagcatc tccggcaacg caacctgggt
     4141 gaaggtcgca acggtcgccg gcgacgatcc gcagattgcc ccaaatgccg cgctgccgcc
     4201 gatcgtcgca taggaaagcc cgccgcgccg atgaccgagc aatgctgcgg ccgcggcgta
     4261 aagatcccgc gaaaagcctg agacattcac gaaagcgccc atcatcagga acagtggcac
     4321 cgccgcaagg tccatgctgc cgagcacttg cgaaggctcg gttgcgagaa acgtcagcgc
     4381 ggaggcccac tcggtctgca gcgcgaacgc aacgatgccg accacaatca tcgcaaaggc
     4441 cagcggaata tgcagcgcaa ccagggcgaa cagcgccacg atgccggcga tgcctatgcc
     4501 ggtcacagtc atgccacgct ctccggctcg cgccgctcgc ggacgcccag cacctcctcg
     4561 atcagcacaa aagtctgcac cgccaccgcc acccaaagga cgccgtcgac gacgaaccag
     4621 aacggcgcct tcggccagtt gagcagccat gtcacgtcgc ccgcccgcat cgttttcatg
     4681 ccgaacagac cgaattgcca ggccatgccg atcatgacga tctcgacaac gagtgccgcg
     4741 agcgtatcga tcccgcgaac gccccagggc ggcaggattc gatcgaagag tcgcagcacg
     4801 atattattcc gatgcaacag cacgatgggc aggcagcagg cgccgcagat cgccgcgatc
     4861 atgtcgccaa tctcgcgcac cagatcgatc ggcgtgttcg cgatcgccct caatagtcca
     4921 tcgagcaggg tgaagacggc gaggatcagc agcaaggaca ggccggcgac ggcggagata
     4981 ttggccaagc ctgtgacgat cgttcgcgcc gccctcatgg ctggttcgcc ctgatccggg
     5041 ccagcagttc gcgcaccttg cccaggagtt cggcgtttcc cgggctcttc gcaacccact
     5101 cggcattgac cttctgatag accggctgca gggccgcgat atcgccctgc gacggcgccg
     5161 taaccgtgcg cttgggatcg gccttgaatc tggcaatgag ctcgtcgtta tattttccaa
     5221 gctcgcggac ataggtgtcg tcgatccacc ggatcgaata tttcgcaatg acgtcctggg
     5281 ccggttgcgg cagcgagtcg aatttcttgc ggttcattag cacggcgagc gggccggcgc
     5341 cgagcggaag aaggtagtcg tggctggtga cgcggtcgat gccgaagtcg acgacggccg
     5401 ccggaaccgt cgtggttgca tcgatcgtgc cgcgtcccat cgcctcgacc acctcgttcg
     5461 gcggcatcaa caccggcacc atgccgagtt ccttcacggt ctggccgatg accgcgccat
     5521 tgatgcgcac cttcatgccc ttgagatcgg caatcgattt gatgggacgg cgggcaaaga
     5581 tcatgtattg cggattcatg aaggcaccga cgacgtaata gtcggaatag ccgtgcagga
     5641 gatttgcctt caccagctct tcataaagct tcacgccctc ggtcagattc tgcaaaaggc
     5701 ccggcaattc gaacacttga tcgtcgggaa agcggccggg cccgaggctc ggattgacga
     5761 aggcgatgtc ggcaaccccg tcaagtatga gctgcggctg ggccggcagc gccttgccga
     5821 gcgcaccgtt cggataggcc tcgatcctca ccgcgcggct cggatcagcg ttcacggcgt
     5881 cgacccaggg cttgatggcg cgcgcgtaat taacctcggt ttcggaagca aagaacgaga
     5941 gcttgagcgt caccggctcg gccgttgcgg gcagcggcgc ccacaccgca aacaatgcca
     6001 gcagcgcctt gcaaagtcgg gtcatccgtt cctcccctga tgatgcggcc ggttcgcttt
     6061 cgcctccggc cattcgttta cgtggcccga gtcatcgccg aaaaataatc gattttcaat
     6121 agcaattttc taaatgatcg attatgcaaa tggcattcgt tcgggccgtg caagaaaaca
     6181 ccagcagttt caatcaataa actcaggaag cgcagaacat gaatctcggt atcgaagggc
     6241 gcaccgccat cgtctgcgcg tccagtcggg ggctgggatt ggcgtgtgcg caggccctgg
     6301 ccgaagaagg cgtccatgtc ctgatcaacg ggcgcgacca ggcgaaagtc gacaagacgg
     6361 ccgccgcatt gcgtgaagtg gcacgtggcg acgtgatcgc gattgccgcc gacgtcacga
     6421 ccacggacgg gcaggcaaag ctgctcgcgg cctgcccctc gccggatatt ctcgtcaaca
     6481 ataatgccgg gccagcgccc ggcaatttcg ccgacatcga acgcgaccgc tggctgaagg
     6541 cgatcgaggc gaacatgatc gcgccgctga tgctgatccg cgcggtgctg ccggcgatga
     6601 agcaacgccg cttcggccgc atcgtcaaca tcacgtccgc catggtgacg acgccccggc
     6661 cgcatatgac cgtatcctca ggcgcgcgcg ctggcctgac cgcggcgatc aagggccttt
     6721 cgctcgaagt cgcgccctac aacgtgacga tcaacaatct cctgcccgaa cgtttcgata
     6781 ccgatcgtca gcgccagatg gccaaggctg cgatggcgcg cgaaaatatt tcctacgagg
     6841 aggcgcgggc gcgtcagatc gagtcgattg ccgccaagcg actcggcgac cccaaggaat
     6901 tcggcgcgac ctgtgcgttc ctctgcagca ccttggcggg cttcatgtcg ggccagaacc
     6961 tccatcttga tggcggatcg tatcctgcgc tgatctgaag cgagccacag atcgcgtcgc
     7021 agacgccaga ggcagcggcg ctaattccgt taccgaagag gtcacactct ctcgagcatt
     7081 tcgcggccaa gcggctgtac caactccgta tcaggggcag cacggacgtg ctcgaaaaag
     7141 cggcgtacag cccgaccgtc agggcgaggt tttcgaactc ccgacccctg gattcggcga
     7201 cggcttactc gtgaggaagc gatggtggaa tttcctgatg aaatcgggaa actggaagac
     7261 gttctgcgat gcattggtga ctagggcaag ggcaggcgga gcgaatgatg acccgattta
     7321 ccaagacatt cccgggcgtg acccgcgaac aggttggtga ggtgaatatt atctaaagag
     7381 aagatcatca gattgatcct aacccggttg tcaaccgccc ttcgcggcgg cttttccttg
     7441 acgtcgcggg gatagtcaac gccggcattt tgaagactgg gatcggccaa agatccaacc
     7501 aattcctgcg tccgacaccc ttccctccag gcttcccctt ggcagacgcc ttgaagatgg
     7561 ggcagcaaag cgctatgtta gacaaaatcc atttttgtct aacatacggg cgaaattcca
     7621 tggcgaaggc atcagcgaag aagcgcacac tgctccgggc gaaacagagg agagctgcat
     7681 ccaagggcaa ctcggtactg ataaagacct ttaagcgtga gcttgtgacc agcgggcttg
     7741 ttgtgagcaa ggcgcgattt gaagcggtca tgctggctgc ggaacaatct ggccttctga
     7801 gcgaaaaagg cagccgcatc ggtggccggg taagtccggc cttggtccgg caggccaagc
     7861 gccagacggg catcgaaacg gatacggact tgatcgagtt tgcattggcg acggttgcac
     7921 ttgaggacaa tttcgcagag gctttcaagg agtctcgggg caaagttgat ccaaccgtga
     7981 agctcggttt ctaaggtggc ggaattcgac tttgacgcgg cgcgacgttg ggcgcggttc
     8041 gatccggaaa acacgttggc acggcgcagc gacgatgaac ttccattcgt cagcgccagc
     8101 ctgatcgccg gacagggact gctgctcgat acctgcgtct atatcgatca aatgcaagat
     8161 cgttcgcctc cggtcctgga cgaccttatc gcgcagcgac aggtcaacca ttcgaccgtc
     8221 gcgatccagg agttgatgca cacggtcggc gtgctgaagc cgtccgacgt gcgaaccgcc
     8281 cctgtcgtcg atgtgatcag gaaccaaatc aaagcgatgc cgccacatcg gatctttgcg
     8341 ccggacatag aggtgctggg acgagccgcc ctgctatcgg ggatcctctg ccgcctccag
     8401 gcttatggaa aggacggcaa gttgcgcgcg cttcaggact gcgtactctt cctgcaggcg
     8461 caaaagctcg gctttgttgt cctgactgca aatgtcggcg actatgatat tctacttcag
     8521 ctcataccaa atggacgcgt gctattctat cgttcgaaat gaacgtacgc ccgcctatct
     8581 ctacgatggg ctacgctgct gccctcttat atgcagcttg tggttttcct tcgcgagatt
     8641 tttggttcaa acagcgctga ccaccacaag agcgaacgct agaagcacat cggagagaag
     8701 ccgacggaaa agctcaaaga ataggttcat tgcgagtaac aatgtgggag tagaaatcga
     8761 gaaaatattt tcgcatcaat gacttatgcc gcttggtgcg ctcggaggga ctcgaacccc
     8821 cacgatttta ctcactgcca cctcaaggca gcgcgtctac caattccgcc acgagcgctt
     8881 ttccagggat cggcaccgga tttgcgtcct gccagatcag accggaccag gaccagatca
     8941 agaccggatc accggcgccg atgtaacaaa tcaatgatgg agggacaagg cctgccgggg
     9001 tggctgttct taccggctgc ccggcagtcg tggcagcatt tgcttgactt cgaccgcgat
     9061 acggttgcga tcgaccagca ccacgccgct ggcgatcggc aggttgttgg cgaggatctt
     9121 gacctcgtcc gcctcggtgg cgtcgagttc gatgatggcg ccacggctca ggcgcatcac
     9181 ctgatgaatg ggcatcgagg tggtaccgag caccaccatg agatcgactg tgaccttatc
     9241 gagcgttggc actgtggaag cgacccgtac agacgattgc gaaattgcgt aatttgagat
     9301 caccacgtta tggttagcca atggttaatg accgccaaag cctcgatttg acgaccctcg
     9361 cgcgccccgc cgacggcggc gcggtcgaat ggcaggtttc ccgtgcgccg gtgccctatc
     9421 tggacgccgt agcagccatg gaggtccggg ttgcggccat cgcggctggc gaagcccccg
     9481 aactggtgtg gctgctggaa caccccccgc tctacacgtc tggcaccagc ggcaacgccc
     9541 gtgacctgct cgacgcccgc tttcctttgt tcgcgaccgg gcgcggcgga cagctcacct
     9601 atcacggccc cggccagcgt gtggcctatg tgatgctcga cctgaagcgg cgccggcccg
     9661 atgtgcgggc ctatgtggcg agccttgagg aatggatcat ccgcaccctg gacgcgttcg
     9721 gcgtgcacgg agaaaggcgc gaggatcggg tcggtgtctg ggtgcagcgg ccggacaagg
     9781 ggtccgggtg cgaagacaag gtcgcggccc ttggcgtgcg gctgcggcgc tgggtctcgt
     9841 tccacggcat ctccatcaat gtggcgcctg acctgacgca cttcgacgcc atcgtgccct
     9901 gcggcgtggt ggacccgcgc tatggcgtca ccagtctcgc cgatctcggc catcccgcga
     9961 ccatggccga tgtcgatatc gcgctccgac gggcgtttga agcggtattt ggtgccgtgc
    10021 gaacgcgcct gccggaaccg atttgaccag gcggcgctcg gtgcggcggg ctacttccca
    10081 aggctcgggc caagggtcga accaaggctc tgaccaaggc tctgaccaag gctgggagcg
    10141 acgcgcatct tccacaggtt ccagatgcgg tcgagcacca agaggttgat cgcgtcggct
    10201 ttcgcgccct cccccggcgt caaataggtg atgtcgccac ccagtgctac ggcttgcgac
    10261 tgcagccggg cgttgacgtc ggtgtaataa gcccggaaaa cggccacttt gacggctggc
    10321 cccacggtca cgtcgccgtg gccgcgcatc aagaccaccg acctgtcgcc gagcgtctgc
    10381 gccagcgatt tgccgagcgt ggggtcgcgg accagcatgt cggtctcgcc aaaatcccgt
    10441 cgaatgtccc atacgggtgc gccagccgcc aggaaggccg cgttgtgata catcggccgc
    10501 aacggcacct ggctcaccgt gaatggaacg acgccgggag aatgcgtatg aaccaccgac
    10561 atcacgtccg gccgcgcctt gtagatttcg ctatgaatga agcgttccag aaacaccgat
    10621 cgaccgctgc tgcccacggc gttgccgtcg agatcgaact ccatgatgtc gtcggcggtg
    10681 accagcgccg gcgcgagcga ccgcgacatc agaaagtggt tgggattggt cgggtgacgc
    10741 gcgctgacgt ggccaaagcc gtcgagaacg ccgaagtcgg cgaggacgcg gctgccgatc
    10801 acgatatctt cgatcaaagc ggcatcgacg cccgaaaccg cgacggacgg cgtttgggcg
    10861 cgcgtctgcc cgaaaccgaa caggagcgcc aacaccagta acattcttga agtcgcggtc
    10921 attgtcatct cccaattgaa ccgctgagca tccaaggctt gttgcatcag gaaattatcc
    10981 gaagcgcaac ccgtcgaacc tgcgaagata agggtgttag cgctgcccct acagaatagc
    11041 ctcgaaggca ctgcgcaagg tttcatgccg aaacacgaac ccgctgctca atgccttgtt
    11101 cgggatgacg cgctggcctc ccagcagaag ctcatcggcg aaatcgccgg ccaggcgatg
    11161 gagcaatgct gcgggaacgc gaaataccgc gggtcgatgc agccgccggc cgagttcctc
    11221 gctgaatttc atgtttctga caggaatcgg ggccgtcgca ttgatcgggc ccgaaatgtc
    11281 ggactttgcc agcgcatgaa cgatcagacg aacgaggtcg tcgcgttcga tccaggacat
    11341 ccactgctgt cctgatccca gcgggccgcc aagaccaaac tcgaatggtg tcagcattcg
    11401 cgtgagaagc cctccttcgg tcccgaccac caggccgatc ctgacatgga cgaccctgac
    11461 gccgtattcc gcggcgcggc tcgccgcctt ttcccaggca tcgcacagtt catggctgaa
    11521 acagccatgg gcttttgcgg actcggtcaa aggctgatcc tgccaaagcc cataccaccc
    11581 gatcgccgaa ccgctgacca gcacggccgg ccggccttcg aggcgcgcaa tcaattgaac
    11641 caatgcgccg gtcatatcga tgcgggaatc gagaatcttg cggcgtttgg cctcggtcca
    11701 caacccgttg ccgatcggct cgcccgccag attcacgatg gcatcgattc tggcatcggc
    11761 aggcagctga tcgaggctgg tgatcaaggt gatcggcggg cccaaggttt cggctttggc
    11821 cggatttcgg accagtgcga tgacttgatg acccgcgtcg gcaagagcca gaacaagcct
    11881 gctgccgatg aagccggttg caccggtgac gaggatggat tgccgtccag gcaattccct
    11941 ggcgaggccg cttacggtcg cgatatttaa ccgcgccaaa cgtctggatg ccgcgaaatc
    12001 ccgcgccccg ccgaggccgg cgccgaccgc ggacaccgtg gcgaggatac tccagaagcc
    12061 atgaaacgcg ggcttgatgg cagcaggctg gccggcccat tcgatcaaga tcggcagcag
    12121 cagaacaagg atgccgccgt aattcagtgc cagcaaggtg tggttgatgc gttcgctcgc
    12181 gggaagtttg cggctcaaat cttcttcaac gaaatccatc aaggtgacga cgacttcgat
    12241 gaccaggacg gcgatcacga tcatcgccca gaagccgtga acttccagcc aaccgagcgt
    12301 caggaacaac agggcgtaga gcgcattgcg cacgctgtgg agctggagtt catggcgctg
    12361 cgaggggcgc cacgccaacc gctccgtcag ttcatggtga tagaacgtat cgaaggcgcc
    12421 catcgcaatc tgaattgcga tcaaggtcca cagaaacggt gacgtcatgg cgttgcctcc
    12481 cggaacacgg cggactgacg aataagggtg ccaaagcggg gatgaatgat ttcaagcgtg
    12541 aatcgaaatt cgccgctgcc gcaatcggaa tgggtcaccg tcaaggcacc cggcgtgagc
    12601 aacgccggca gcggcaacct caggcgccca acctgcacaa aataacccgc gctgcggaac
    12661 tgcagggccc ggccatcgac aagcatccgc agcgccatgc tgacgccgaa cccgacatat
    12721 tcctcgagac ctgtcggtcc cgagaaacgc ttcgatgagt ggatgacttg gggaaatccg
    12781 ttgcggcgcg cgtaaatgcg agtccagacc tggccaccgg tcgcagcatc ttcggttacc
    12841 gtgacaatgc ttgggacatg ggtgtcaccg ccagtcggca gagggccccc gatcaatcgc
    12901 gcaagctgcg ccagccacca tccgatccgg ctgaacctga cctcgacgac ctcaccgacg
    12961 taaatggcgg tcttgccatc ggcgagacgc ttcgagaacc gtcgccaggt ggccagcggc
    13021 agcctccccc agtcctcgtc gggcagcaaa gcgcgaaatc tgagatcact taggttgccg
    13081 tcgagacttt cgtcggaagc cgcaggcgct cgaagtttcg ggatcttgct ttgtgatgcc
    13141 acaatgcact ccagcaatct caaaccttat ttggattttc ccgccggcaa gaaactgacg
    13201 atcttttcgc cgagcttgat caaggcaatc aaccttggcc gcggcacggt cagcatctgc
    13261 gcgtaccagc gatcgaccag ttcggtgaaa gccagcattt ccttcagccg cttgctcgca
    13321 actggactga tcgtcggatc gtcggcggcg tcgaccacgc aggcgcgcaa cgcatcgacg
    13381 gcggggtcga tctcgcgctc cttccggccg gcggcaatcc gcgcggccac ctcccagata
    13441 tccgtctccg cctcatagtg atcgcggcga tcgcccatga tcggcacgcg ccggatcaaa
    13501 ttccaggaca acaattccct gatggaattg gagacgttgg atcgcgccat gcccagcgtt
    13561 tccgcgatat cgtcggcggt catcggggtt tccgacaggt aaagcagacc gtgaatctgg
    13621 ctcaccgagc gattgacccc ccactcatcg cccatgtcgc cccaatgcag aacgaaccgc
    13681 tcgacggcgg caggcaattt cttcttggct gttatttctg tcatgacaga aatatctgac
    13741 gagagcagac gaatgtcaat ggaaattctc aagtcaatgg aaattctcaa gagagacacg
    13801 ccttggtcgt gaataaggtc cgttgccagc ccatcgcttg ccagcggcct tgcccggccg
    13861 cgcaatgtca ctagaactga tgttggggac ggccgacccg cgccgctctc cacgcggcga
    13921 tccgcgtcgc gctcagaaca ccgtcgaggg agatatgcat gagagttttg tgtgccgctg
    13981 cgatgattgc gctgctggcc gggcctgctt atgcccaaca aaaatcggta cccaaatacg
    14041 gggatgttac acccgacaaa agcccgaacg agatcgagca ggatcgcgcg gccgagcgag
    14101 cctataagcg gtcgctcggc aacattcccg accagggccc taccgacccc tggggcaacg
    14161 cgcgcagcgt ggatgcgccc aaggccgtcg ccaagccgtc tgcggcgaaa ccgcggacca
    14221 aaaccggcgc ctccgcgaat tgaagtacga tcccggacca agtccggggc aggctttcgc
    14281 ttgaaaacaa ctttaaatcg tcggcagaac cgaaaaccgc tcgccgggct gccgcaaatt
    14341 cagcgcatcg gcatcgccgg ggtgctggtc gatcgcatcg acgcgcgccg atgacgggcc
    14401 gcgccggcac gacgccacca tgttcgacac gacatcctcc ggccccgcga acaacgcctc
    14461 gacgctgccg tcccggcggt tgcgcaccca tccctcaagt ccgtgcgcca ccgcctggtg
    14521 ctcaacccag taccgatagc caacgccctg caccctgccc cggatcgtca cctgacgaat
    14581 cgtgccgccc atctatcgtt tgagccccaa aaattcggcc gcgcgcactt tcatatctgc
    14641 gtcgcgcgtg acgcggccgg tcaggtcgga tgacgcaagg cccttcaaat tcttcggcga
    14701 ctggccgtcg cacagcgcct tcagcgtctc gtaaacggcc aaggtcgccg cggcaattgg
    14761 ggcgtgcccc tgcaacgcga ttcgcaccct tttgccggcg agatagtccg ggtcgatcat
    14821 ttcctcgggg aagccgccca gcacgatcgg cagcgttgtg gcggcagcga tggcttcgag
    14881 ttccgcgcgg tttttcacgc cggtgaagaa caatgcatcg acgccggcgg cctcataggc
    14941 ccgggcgcgc ccgatcgcgt cctcaagacc ggagaccgac gcagcaccgg tgcgcgccat
    15001 gatcaccagc gatggatcgc cgcgcccgtc cagcgcggct ttcatcttgc cgacaccttc
    15061 ctccagcgaa ataagctgcg ttttcgcctg cccaaaggcc tgcggcagca gggtgtcctc
    15121 gatggtgagg ccggcggcgc cggccgtttc cagttcctga accgtgcggc ggacattcag
    15181 cgcattgcca tagccatggt cggcatcgac catgaccggc aacgcggccg cgcgcgacat
    15241 ccgccgcatc tgttcggcca gttcggtcag cgtgatcaac gcgatatcgg gatctcctag
    15301 caccgcgagc gaagccaccg atccgccgaa catgccgacc tcgaaaccga ggtcctcggc
    15361 gacgcggatc gaaatcgcgt catagaccga ccccggccgg atgcaggtcg atcccgccag
    15421 gatcgaacgc aacgcttcgc gacgtttgcg gaaagccatg cctgcctctc tcataattgc
    15481 gcgtcttctg gtcgcaaaac cggtatccac tttcgcggaa tacgcgggct tagacaaatt
    15541 cgagaatgag cgcatccacc gccagcgttg cgccggcggt tgcgtgaatc ttcttgaccg
    15601 tgccgtcgcg ttcggcgcgc agcacattct gcatcttcat cgcctcgacc accgccagcg
    15661 tttcgcctga tttgacctcc tggccctcgg caaccgctat cgacaccacg agccccggca
    15721 tcggacacag cagcttcttg ccggtatcgg tcgcggtctt gaccggcatc agccgcgccg
    15781 cggcggcctc ggactcggtg aagacattga ccgccacctc aaaaccctga tgcgcgaggc
    15841 ggaagccatt gggaatcggg cgcacctgca tggccaccgg atggccgtcg acgctaccct
    15901 gccagaccgg atcgcccggc acccagggcg agaccagttg atgcggatgg cccggattgc
    15961 tggccgcgcc gacaaaacgt accgtaataa aatcgtcttc ccgcgcaata tcgagcgcga
    16021 tttcgttgcg gtccagccac accgcacggc ggcgctcgcg ctgcactgca cggtggatca
    16081 gttggccgga aatctgccgc ttgcgctctc cgagcacgtg atcgatggcg gccgcgaccg
    16141 cggccagccg acgggcaacc tcgccttccg ggacccgaac cgcaaacccc ctgggaaact
    16201 cctcggcaat gaagccggtg gaaagattgc cctcgcgcca gcgcggatgg agcatcaatg
    16261 ccgacaggaa cggaatgtta tggcggatac cgtcgatgta gaatgaatcg agcgcggtgg
    16321 cctgcgcctc gatcgcggcg gcgcgcgacg acgcgtgggt cacgagcttg gcgatcatcg
    16381 gatcgtaata gatcgagatc tcgccgccct cctgcacgcc ggtatcgttg cgaacggtaa
    16441 tgccgtcgat gctgctctcg accggcgggc gatattttac cagccggccg atcgagggca
    16501 ggaaattgcg gaacggatct tccgcataga cccgcgactc caccgcccat ccgctcaact
    16561 ttacgtcctt ctgtttcagc gccagctttt cgccggcggc aacgcggatc atctgctcga
    16621 ccagatcgat gccggtgatc aactcggtca cgggatgctc gacctgcagg cgggtgttca
    16681 tctccaggaa gtagaaactc ttgtcctggc ctgcgacaaa ttcgaccgtg ccggcggaat
    16741 cgtagttcac ggccttggcg agcgcgaccg cctgttcgcc catcctgcgg cgggtggtct
    16801 cgtcgagcag cggcgacggc gcttcctcga tcaccttctg attgcgccgc tggatcgaac
    16861 attcgcgctc gccgagataa atcacattgc catgcttgtc gcctagcacc tgaatttcga
    16921 tgtgacgggg atcgatgatg aatttttcga tgaatacccg gtcgtcgccg aacgacgcct
    16981 tggcttccgc cttggcgagg ccaaagcctt cggcgacctc cgaggtcgaa tgcgcgatcc
    17041 gcatcccctt gccgccgccg cccgcggagg cttttatcat gacgggataa ccgatgccgt
    17101 cggcaatctt caccgcgtgc ctttcgtcgg tgatgatgcc aaggtagccc ggcacggtcg
    17161 agacatcggc cttcgcggct gccttcttgg attcgatctt gtcgcccata gcggcgatag
    17221 cgcccgcgtt gggtccgaca aagacgatac cggcctcgct cagcacgcgc gggaatgctt
    17281 cgcgctcgga caggaagccg tagcccggat gcacggcttg cgcgccggtc ttgcggcagg
    17341 cctcgacgat cttctccatc acgagatagc tttcggccgc ggccggcggc ccgatcaaaa
    17401 ccgccttatc ggccatttcg acgtggagtg cgtcgcggtc ggcttcggaa tagacggcca
    17461 cggtctctat tcccatgcgg cgggcagtct tgatgacccg gcaggcgatc tcgccgcggt
    17521 tggcgatcag aattcgtttg aacattttct tgactgcagc ctttgggcgg gatccggagc
    17581 cacaaactgg cactcagggg cgtaacaatt ccgtcgtaca gcaaaaccaa acagaggcaa
    17641 cggcttatat gccgaaaatc cgcttgtcag ggtagatatt cccgcgccgt tcagtcaggc
    17701 tccgcagcga gggggcggcc cccatcctcg gtatcggttt cgcgtttcac gcccaccgcc
    17761 gaaccgggtt ctctctacat gatgcgcggc gaatccaggc agatctggca gcacagcatc
    17821 cccgcggtag agtagcgccg atactcgatc accttccgga cgatgaggga gaagatgctc
    17881 gcaagaggga ttttggaggg tccatgattg ttggacagcc catggacaac ccatgtaaca
    17941 aatccgtcat acagcaaaac taaagaggca acggctgata cgccggaatc tgtttccagg
    18001 gggagatctt catgcgccgc tcaatcctat tgctgtccgc tggcatgctc gtgctgtccg
    18061 ccggcatggc atcagcgcag aacagcacgc cccataatct gatcctgttt gtgccggatg
    18121 gattgcgcgc cttgaaggtc acgcccgaaa ccgcgccggc gatggcggaa gtccgcgaca
    18181 agggcgtcaa tttcaaaaat ccgcactcgc tgttcccgac cttcaccatg gcgaacggat
    18241 cggcgatggc gaccgggcat cacctcggcg ataccggaac gttcagcaac accatctaca
    18301 ccggtttttc gtcggcgccc gccggcgaca ccgtcgtccc cttcatcgag aacgacgctg
    18361 tgctgggcga tgtcgacgag catttccacg gcgattatct gaatgaggaa accatcctga
    18421 aaatggcgcg tgccaagggt tttagcacgg cggcgatcgg caaggtcggc ccgacgctga
    18481 ttttcgacca caccgaccgc gccgatagac ccggcctgca ttcgatcgtg atcgacgacg
    18541 ccaccggcag caagaacggc gtgccgttgt cggaggagat gaaggccatg ttgacaaaag
    18601 ccaatcttcc gctcgcggca ccgtcccgcg gcgacaacgg caaggcaggc gacgccaaga
    18661 cccccggcac catggtgccg aataccgcgc aacaggccta tttcgccgat gtcgccagcc
    18721 aaatcgtgct gccgctgttc aaggagcgcg caaagccgtt cgtgctggtg ttctggtcgc
    18781 gcgatcccga cggcagccag cacaacaccg gcgacagcct caacaccatc acgcccggca
    18841 tcaacggccc gacctcgctc gccggcatca agaacgccga caacaatctg gcgcaattgc
    18901 gcaaggcgct cgacgatctc ggcctctccg ccaccaccaa cgtcatgatc gccgccgacc
    18961 acggcttctc gaccatttcg aaggagagca agaccagccc gtcggcgaaa gcgacctatg
    19021 aagacacccc gaaggatttc ctcccgatgg gttttctcgc gatcgatctg gcgaaagcgc
    19081 ttgacctgcc actgttcgat ccgaacgaca agaacgcgcg tattgcggaa aatgcccacc
    19141 ccaaggccgg caacggcgtg cttgggcagg atcccgcaaa acccgatctg gtggtcgcca
    19201 ccaatggtgg atccgacctg atctatttgc cgaaaaaaga caagaagctg gcgggacgcg
    19261 cggtcaaggc gctgctggag caggattacg tcagcggcat tttcgtggac gacgcgcttg
    19321 ggcgttatcc cggcgcgctg ccgctttccg cgctcaatct caaaggcaag gcggtcacgc
    19381 cgcagccgtc gatcgtggtc aatttccgct cctacgccag cggttgcgac gaaccgacca
    19441 catgcgcggt ggaggtagcg gacaccgtgc tgcggcaggg acagggcatg catggcagtt
    19501 tcagccgcgg cgacaccatg aatttcatgg cggcgaccgg gcccgatttc aaggcggggt
    19561 atgtcgatgc gttgccggtg agcaacgccg atgtcgggat gaccatcgcg cagctgctgg
    19621 atttgcatgc ggcagccgtc ggcggactga ccggccgggt gatgtcggaa gccttgccaa
    19681 acggaatcgt gtcgaagttt gccgacggca cgatcagttc aaagcccgat ccgaacggct
    19741 tgcagacggt cgtgaaattc cagcgcgtcc tctcgcagcg ctatttcgat gttgcgggat
    19801 tccccgggcg gactgttggg cttgaggccg gcaagcaaaa aacggcgggg aagtagctcg
    19861 cgccgctcag gccgcgtcca tcatcgacat gatgtcctgg cggttgccgg agcgcgacga
    19921 gagttcgtag acgagagttc gtggggcggg ttggagccga aggcgtaacc cgtcatcttg
    19981 ggggttcata acggcggatc aagatccgcc ctacaaactg ttttttagta agcgtatcag
    20041 ctatttaagg tataaaacta gaccatgact gaaggttcta acgatgtgtt gcccagcagc
    20101 gccgtcccgc gggcacttta tcaaatttcg gaatttccga attttacttg acggcccggt
    20161 aggaacagga gtattgcgat ttggtcccgt cccactcaga ggggcggctc gcgatcgtca
    20221 cggacgcggg gcgggatgcg gtggacgcgg atgcgctctt gacgaatggc gcggaggcgg
    20281 acggagaagt cgtgtggtcc tgacaccccg acgctggtgt caagttcttg agaaacaagt
    20341 ttctcaggga tgacggtggc aagaaagccc ggtcgccggg gagagcacga aggaagccgt
    20401 taaaaccatt gcgcggggaa tgccgggtga accggtgtga ccggactaac gcgtgtgctc
    20461 tctaccacta cctctgcaca cgcggcagcg ggcgcatcgg gcgcccggca ttccctgcgc
    20521 cctctgattg gagagggcgg caagtcgcta gccaaactcg ggcgtctcgg gccgcgagat
    20581 cgcgaaggcg tgtctgagaa cacgaaggcg tgtctgagaa cacgcggttg tttgaaaatt
    20641 gagcctgaag caaatcacgg catgcgcgcg cggtgcggtc atcgtcgttt ccgatgggat
    20701 gaattcctat gccggccgat aatgagaaac ggcggggcaa taaccccgcc gcttgcgata
    20761 tccgggcctc aaaagctgaa tttacattcc gatgtcgaac acgttcggca gctgcgagaa
    20821 cggcagcgcc gccgcgccga caatggtggc gaccattcgc atcaaggtgc gattgctgcg
    20881 gtgcttgccg cgcatctggc ttgcgatcca ctccagcacc tcggtatcga cgccggcagt
    20941 ccttgtcggc gcccagcttt cgatcgcggt gtccggcgac agcgcgacgg tgcgcgatgg
    21001 caccggcaga ccggactcgg aggccgcgtg gtgcacgacg gccttcgcca gcaaatcgtc
    21061 gaaacgcccg tcatcgctgc gctcagcggc agcgtcgttg atctcaaaca gcgcctcggc
    21121 ttccgcccgg ctgaccggct cgtgctcgac cgcggtcgcg gtcaggatgc gcgcgcacca
    21181 ggcggcgtca tcggcgtcca gcgcacggga aaaatgaacg cgacctttgg tggtcggacc
    21241 ttcgccggtg attacgccat cgcgcacgac agtgagggca agtgccgcgc tatcgcggca
    21301 ggagatctca gatggttcga gggcgggttc ggcgggcatt ccggcagagg cagacattgg
    21361 agttctcttt tgtctttttc caatcccagc aattttccat gccgggcgtg aacgagtggt
    21421 taacgattcg gaacttgcgt gccgactctt gtaaacagtt gctgattggt cgctgtgacg
    21481 tccgtcacgg tcccagaggc gccgcaaact ggcccggcaa tggtgcggtg cgcaagaggc
    21541 aaaatcggga taatgatgct tttgaagaca ttgtgatttt tccgtcgcag tggggggtat
    21601 taaaagatgc tgctaattga actctcaact ggagaagcat gttttccttt tgcaggaacg
    21661 gttccattga gccgccgtgc gccctatatt gaagccgaat ggctacttcg cgggttggaa
    21721 cgagcgcgtg agcgatgaag gctcaactgg cacggtttgt cattttccag atagcgatgc
    21781 tggtcgcgct tgttatcttc atgacaatgg atgtcggctg gctatccgcc gtgctcatcg
    21841 tcatatcggt catagccggc cttagcctta gcgatttggc ctttcaccgc ctcgccgacc
    21901 cggaaacaat ccagcgcgat ctggaagagc gggtccgcaa tcagcacctc taacgccggc
    21961 cgtccggcaa tttcatccgt aacccgaacg atatgaggtc aaaaatggca ctccagattg
    22021 gcgctgtggc cccggatttc gaggctgaaa ccaccgaggg caagatcaat ttccatgact
    22081 ggatcggcaa cagctgggcg ctgttgttct cgcatccgaa ggatttcacc ccggtctgca
    22141 ccaccgagct tggcgcgctg gccaagctga agccggaatt cgacaagcgc ggcgtcaagc
    22201 tgatggggct cagcgtcgat cccgtcgaca agcacgcgaa atggtccgaa gacatcaagg
    22261 agacccaggg tgctgcgccg aactatccga tgatcggcga caccgatttc aatgtatcga
    22321 agctgtacga catgctgccg gcctcaacct ccggcgatgt cgccac
//
LOCUS       DATNOI010000355         8532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2448538,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000355 DATNOI010000000
VERSION     DATNOI010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2448538"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /gene="argS"
                     /locus_tag="VN968_14235"
     CDS             complement(<1..1265)
                     /gene="argS"
                     /locus_tag="VN968_14235"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXL30430.1"
                     /translation="MAKKTAKKAKKAAKKSVKKKLAKKKLATTKSAGRPVEKPAKKST
                     KKTAKKATPKASPAKGAATRAAKGRARKAAAKSRVSPTSGKTATAPLAEDEASAEAIR
                     ATEKPVKESTKPLPKETGRGAIDKPPAHPKPTAKPAPEPHLFANLLARVHAVCRTLAE
                     ESGWSADVDLSRVVIEPPRDAAHGDMATNAAMVLARDAKAKPRDLAEQIAAKLRADDL
                     IASVDVAGPGFINLTLKASVWSDALRTVLREGDGYGRSGIGAAEKVDVEYVSANPTGP
                     MHVGHCRGAVFGDALCSLLQFAGYAVTREYYVNDAGAQVDVLARSAFLRYREALGEDI
                     GEIPEGLYPGDYLKPVGRALAAEYGDKLLAMTEGNWLPAVRAKAIAMMMEEIKGDLAA
                     LRIKHDVFFSERSLIETGNNRVTETIDYLR"
     gene            complement(1298..2506)
                     /locus_tag="VN968_14240"
     CDS             complement(1298..2506)
                     /locus_tag="VN968_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HXL30431.1"
                     /translation="MSVGMAAPRSVFACDPDRSRGRLFAEPPSKTRSPFRRDCDRVIH
                     STAFRRLKHKTQVFVFHEGDHYRTRLTHSLEVAQIARALARQLGLDEDLTEALALAHD
                     LGHPPFGHAGERALNACLQAHGGFDHNAQTLRVVTSLEHRYPDFDGLNLTWETLEGIV
                     KHNGPLMERSGAPVGRYREHGIPAGISEYNRIHDLQLWSFASPEAQAAAIADDIAYDA
                     HDIDDGLRAGLFTVDDLRIIPLAAEMIADIARHYPGLDDDRRGAELVRELISRLIGSV
                     VSEARRRLDAAQPKSVDDVRSHGEVLIAFSADTGEAEAAIKSFLKLRMYRHSRVMRVM
                     GDAEQILFDLFARYQKVPDDLPPEWRPPDGTNSETDRARRIGNFIAGMTDRFALTEHQ
                     RLFDSTPDLR"
     gene            2618..2947
                     /gene="erpA"
                     /locus_tag="VN968_14245"
     CDS             2618..2947
                     /gene="erpA"
                     /locus_tag="VN968_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HXL30432.1"
                     /translation="MTTAVTISERAARRIGEILKTEGDGAMLRISVEGGGCSGFQYKF
                     DVERAQAEDDLVIARDNAVVLVDPASVPFLAGSEVDFVDDLIGASFRVVNPNATASCG
                     CGTSFSI"
     gene            3196..3948
                     /locus_tag="VN968_14250"
     CDS             3196..3948
                     /locus_tag="VN968_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30433.1"
                     /translation="MKPSNGYRGFVDGLSVLRGPWLRPLRAAALTILLLVVPVGAGTA
                     NATVSAAPATGAHVYLLRGVLNIFSLGLDDIAAKLERQGIPVTIANFVSWSSLADDAA
                     AEYRSGKVRTIILVGHSSGATALPDMVARLGQLGVPVKLAIGLDSVFRTRLAGNAERY
                     INFYVASGPGEPVTRTKDFRGTLENVDVQAVPGVGHLSIDKNQIMQQKVINAIEAAVR
                     HPAAASVAQTPGQRELTTARQSASPSGAAARY"
     gene            4227..5174
                     /locus_tag="VN968_14255"
     CDS             4227..5174
                     /locus_tag="VN968_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXL30434.1"
                     /translation="MNSPAPVVTFVIGAMLSASVLCGSFDPASAGQIAVSSNAVPETA
                     ALPPATNPPASEPHARAYLFRGALGPIFSRGMDRLTEKIEHAGVTANVYEFTICRLVA
                     DSAVREYRRDPAPIILIGHSMGGYCALKFAEVLQAENISVSLIVTVDPAHVTPNVPLN
                     VERYINIFLSKSVLGGGDVKPAQGYQGDYASFDLSEQEDVTHINIDKMDLIHQQIVNK
                     IVELGTTRAKADGEAVPVRYVVPPNTAIELWDSGTPVVVRPGDTLQKLATLRHLPLWS
                     LTQINRESDSAPLVPGQRVIVPRHLVPFAEVSAPPPPKR"
     gene            complement(5198..5824)
                     /locus_tag="VN968_14260"
     CDS             complement(5198..5824)
                     /locus_tag="VN968_14260"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXL30435.1"
                     /translation="MSFDPHSLLAIAWSGWTATWPTQLLAIIWITWLVSWVVASFWSG
                     RTQKQVMTWDSQAYRIPILAGAILFTPWTAQILGESPLWHFGNPGIYVLSALTLAGIS
                     FTWWARIHLGRFWSNSITRKEGHQVIDTGPYGLVRHPIYTGLIAGMLATGIAVGTVTA
                     MLGAVLISLGMWQKARMEEGFLTNELGADAYGSYCRRIPMLVPFLPPR"
     gene            complement(5851..6903)
                     /locus_tag="VN968_14265"
     CDS             complement(5851..6903)
                     /locus_tag="VN968_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXL30436.1"
                     /translation="MTSVLITGGSGFIGRHLVSTLVERGLQVRVLDIHPPSHPEPEVE
                     YVEGSVLDPDRVGEALRGVEQVYHLAGLPGMWMRQKSDFHAVNCRGTEVVLAAARRQG
                     TRRFLHCSTESILFRSSASEEAGADHTLPMADHMPGPYTRSKMLAEQRAMQAAASGFP
                     VVIGTPTMPIGPHDHNLTPPVAMLRHFLNRRIQLYLDFIVNLVDVRDVATGLVLAMER
                     GQVGHRYIFGGEGIRLRQVLRLVTENSGRRHLLIPVPGKVAETFAGLLELIADHVTRQ
                     PPSGTSEGVRIARRATSLSIEKAQRELGYAPRPVEPALRETIAYLRDAAGDQQRFDRA
                     GAPRVRPAHAGRRYGS"
     gene            complement(6900..7463)
                     /locus_tag="VN968_14270"
     CDS             complement(6900..7463)
                     /locus_tag="VN968_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HXL30437.1"
                     /translation="MNSYFRRQLADYVEYHRDWWNCVMHVFGIIFLFLAAILPLNFWS
                     VHVFGVQTTMAAMMVLPVLIYWLLLDTALGMGIVGAAVLLLSTAAIIVSNVSVGAVWS
                     ISAVLIVVGVSLQIIGHRLFERRQPALLDNPPHLLLGPMFVMAKLFIALGFRHDLALI
                     IDPVPQTAALGTTFYADAHNGETHPNS"
     gene            complement(7735..>8532)
                     /locus_tag="VN968_14275"
     CDS             complement(7735..>8532)
                     /locus_tag="VN968_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXL30438.1"
                     /translation="NNQTIPLDAVAKLAPTVGPLLINHQGQQPAVTISFNLAPNYSLG
                     YAVDQITSIEQSSNLPATIATGFSGTAQVFQDSLRGQGVLILAAVFAAFVILGILYES
                     FIHPITIISGLPSAGIGAILTLMLFNMELSVIAMIGIVMLVGIVKKNAIMMVDFALER
                     RRVGLSAEHAIREAALLRFRPIMMTTFAAIFGTLPIAIGAGAGAELRQPLGIAVVGGL
                     CVSQLLTLFITPVIYIYLDRIDRRLKRRLEPQAEIENVERPHVAAAE"
BASE COUNT         1663 a         2614 c         2626 g         1629 t
ORIGIN      
        1 cggaggtagt cgatggtctc ggtcacccgg ttgtttccgg tttcgatcag cgaccgctcc
       61 gaaaaaaaca cgtcgtgctt gatacggagc gcggcgagat cgcccttgat ctcttccatc
      121 atcatcgcaa tggccttggc ccgcaccgcc ggcagccagt tcccttccgt catcgcgagc
      181 agcttgtcac catattcagc ggccagcgcc cgccccaccg gctttaaata gtcgccggga
      241 taaagccctt ccggaatttc gccgatgtcc tcgccgagcg cctcgcgata gcgcaggaag
      301 gccgagcgcg cgagcacgtc gacctgggcg ccggcgtcgt tgacgtaata ttcgcgggtg
      361 accgcatagc ccgcgaactg cagcaggctg cacagcgcgt cgccgaacac cgcgccgcgg
      421 caatggccga catgcatcgg cccggtcgga ttggctgaaa cgtattcgac atcgaccttc
      481 tcggccgcac ctatcccgct tcgcccgtag ccatcgccct cacgcagcac ggtgcgcaac
      541 gcgtccgacc agaccgacgc cttcaaggtg agattgatga agccgggacc ggcgacatcg
      601 accgacgcaa tcaaatcgtc agcgcgcaac ttggctgcga tctgctcggc cagatcgcgc
      661 ggttttgcct tcgcgtccct ggccagcacc attgcggcgt tggtcgccat gtcgccgtgc
      721 gcagcatcgc gcggcggttc gatcacgacg cgcgacaggt caacgtctgc cgaccatccg
      781 ctttcctcag cgagcgtgcg gcaaaccgca tgaacgcgtg ccagcaggtt ggcgaaaaga
      841 tgcggctcgg gcgcaggttt ggcggtaggt ttcgggtgag cggggggttt gtcgattgcg
      901 ccgcgcccgg tttccttcgg caacggcttg gtgctttcct tgacgggttt ttcagttgcc
      961 ctgatcgctt cagcggaagc ttcgtcctcg gcgagaggcg cggtcgcggt tttgccggac
     1021 gtcggagaaa cccgggattt cgcagccgcc tttctggcgc ggcctttggc agcacgcgtc
     1081 gcagcgccct tggcagggct tgctttcggt gtggcttttt ttgcggtttt tttcgtggac
     1141 ttcttcgctg gcttctcgac cggcctcccg gccgattttg tcgtggcgag cttctttttg
     1201 gcgagttttt tcttgacgga tttcttcgcc gccttcttcg ccttctttgc cgttttcttc
     1261 gccattattg cctgctcttc cggaggtggc tcgccgccta acgcaaatcc ggggtcgagt
     1321 caaaaagcct ttgatgttcg gtcagggcga agcggtcggt cattccggcg atgaagttgc
     1381 cgatccgcct tgcgcgatcg gtttcgctgt ttgtcccgtc cggcggccgc cattcgggcg
     1441 gcagatcgtc cggtaccttc tggtagcgcg cgaacaggtc gaacaggatc tgttcggcat
     1501 cgcccatcac ccgcatcacc cgcgaatggc ggtacatcct gagtttcagg aacgacttga
     1561 tggcggcctc ggcttccccc gtgtcagcgg aaaacgcgat cagcacctcg ccatggctgc
     1621 ggacatcatc gacggatttc ggctgcgctg cgtccagacg cctgcgcgct tccgagacga
     1681 ccgatccgat caagcgcgat atcagctcgc gcaccagctc cgcgccgcgc cggtcatcat
     1741 caagaccggg ataatgcctg gcgatatcag cgatcatctc ggcggcgagc gggatgatcc
     1801 tgagatcgtc gaccgtgaac agcccggccc gcagtccgtc gtcgatgtcg tgggcgtcat
     1861 aggcgatgtc gtcggcgatc gcggcggcct gcgcctccgg cgacgcaaag ctccacagtt
     1921 gcagatcgtg gatccggttg tattcggaaa tgcctgcggg aatgccgtgc tcgcggtagc
     1981 gcccgaccgg cgctccgctt cgctccatca gcggcccatt gtgcttgacg atgccctcca
     2041 gcgtctccca ggtcaggttc agtccgtcga aatcgggata gcgatgttcc agcgaggtga
     2101 cgacgcgcag ggtctgcgcg ttgtgatcga atccgccatg ggcttgcaga caggcgttga
     2161 gggcgcgctc gccggcatgg ccgaacggcg gatggccgag gtcgtgggcc agcgccaacg
     2221 cctccgtcag atcctcgtcg agaccgagct gccgggccag cgcgcgggcg atctgcgcca
     2281 cttcgaggct gtgggtcagc cgggtgcggt aatgatcgcc ctcgtggaac acgaagacct
     2341 gggttttgtg cttcaggcgg cgaaacgcgg tagaatggat cacccggtcg caatcgcgcc
     2401 ggaacgggct gcgggtcttg ctcggcggct cggcgaacag ccgcccgcgg ctgcggtcgg
     2461 ggtcgcaggc aaagaccgag cgaggagcag ccattccgac cgacacggcg tattgttccc
     2521 taaaaggttt gattccagcg tctggcgcac ttaactatgc tggaagccgg ataccaaatt
     2581 atttggccat tggcatcggc gattggagac ctctcacatg accacagccg tcacaatcag
     2641 cgaacgggcc gcccgccgca ttggagaaat cctcaagacc gagggtgacg gcgccatgct
     2701 ccggatcagc gtcgagggcg gcggctgctc gggctttcaa tacaagttcg acgtcgagcg
     2761 cgcgcaagcg gaagatgacc tcgtcatcgc gcgcgacaat gcggtggtgc tggtcgatcc
     2821 ggcctcggtg ccatttctgg cggggtccga ggtcgatttc gtcgacgacc tgatcggcgc
     2881 ctcgttccgg gtcgtcaacc cgaatgccac cgcgtcatgc ggctgcggca ccagtttttc
     2941 gatctgagaa ccttctcaat caaatcttct caatcagacg acgtcggcag cacttcgccg
     3001 agttgagttc catatccgca cgaagcagga attggcttgc cgatgctgtt gcaagcctga
     3061 cacgcatccc ggataaaagt ggctcgcgtg acctgaccct gttgcagttg cgggccggcg
     3121 aaggctacgt tgctttgcac aacatcgtgc ataatattcg gtacgcctga ttcggtacgc
     3181 gtgcgaggag gaccaatgaa gccatccaac ggctacaggg gatttgttga cggactttcg
     3241 gtcctgcgcg ggccgtggtt gcgtcctctg cgagcggctg cgttgacgat tctgctgctg
     3301 gtcgttccag tcggagccgg cacagcgaac gcgacggtct ctgctgctcc cgcaacgggc
     3361 gctcatgtct atctcctccg cggcgtgctc aatatcttct cgctgggcct ggatgatatc
     3421 gcggcgaaac tcgagcggca gggcatcccg gtcaccattg ccaattttgt ctcatggtcg
     3481 tcgctcgcag acgatgccgc ggcggaatac agaagtggaa aggtgcggac catcattctg
     3541 gtcgggcact cttcaggcgc aacggcgctg ccggatatgg tggcaaggct tggtcagctt
     3601 ggggtcccgg tgaagctggc gataggcctc gactcggtgt tccgcaccag gctggcgggc
     3661 aacgccgagc gctacatcaa tttctatgtc gccagcggac ccggcgagcc agtgacgagg
     3721 accaaggatt ttcgcggtac gctggagaac gtcgacgtgc aggcggtgcc cggtgtcggg
     3781 cacctctcga tcgacaagaa ccagatcatg cagcagaagg tgatcaacgc catcgaggct
     3841 gcggtcaggc acccggcagc ggcgtctgtg gcgcagacac cgggccagcg ggaattgacc
     3901 acggcgaggc agagcgcgag ccctagcggc gctgccgcgc gctattgaac ccaagctccg
     3961 gaccgcgcgt ccggcccaaa gcgcgcctca aattctgcca attcgtcgga gatacagacc
     4021 gtcgtgcggc ggatggcgca ctttcggcga tggctttgct gacatcggcg gctgtgcgtg
     4081 gtgggcgaaa atcagacgga tgagtccgtg acaaattccg cggcgccagg cggaaggacg
     4141 ttggcggggt tttatctgtt cgattgatcc gccagcgtct agggtgtctc gcgtgcctgt
     4201 tcgtcgaagg tacaagcagg aagcgcatga acagtccggc gccagttgta acatttgtga
     4261 tcggggcgat gctatcggca agcgtcctat gtggcagctt cgacccggcg tcggccggcc
     4321 agatcgcggt gtcgagcaac gccgtccctg aaacggccgc actgccgcct gccaccaacc
     4381 cgccggcgtc agagccgcat gcgcgcgcct acctgttccg tggcgctctt ggtccgatct
     4441 tttcacgcgg gatggaccgt ctgaccgaaa agatcgaaca cgccggcgtc accgcgaatg
     4501 tctacgaatt cacgatctgc cgcctggtcg ccgattcggc ggttcgcgag tatcgccggg
     4561 atcccgctcc gatcattctg atcgggcact caatgggcgg atattgtgcg ctgaaattcg
     4621 ccgaggtgct gcaggccgaa aacatatcgg taagcctgat tgtcacggtc gatccagctc
     4681 acgtcacgcc caacgtgcca ctcaatgtcg agcgctacat caatatcttc ctgtcgaaaa
     4741 gcgttctggg cggcggcgac gtcaagccgg cacaaggcta tcaaggcgac tatgccagct
     4801 tcgacctgtc ggagcaggag gacgtgacgc acatcaatat cgacaagatg gacctgatcc
     4861 accagcaaat cgtcaacaag atcgtagaac ttggcacgac ccgggcaaaa gccgacggcg
     4921 aggccgtgcc ggttcgctat gtcgttcctc ccaacaccgc catcgagctg tgggacagcg
     4981 gtactccggt agtcgtacgt ccaggcgata cgctacagaa gctcgcgaca cttcgccatc
     5041 tgccgctctg gtcgcttacc cagatcaacc gggagtccga cagtgcgcca ttggtgcccg
     5101 gtcaacgcgt cattgttccg cgtcatcttg ttccgttcgc tgaggtctcc gcgcccccgc
     5161 cgcccaagcg ctgacgcacg gcctgtcaat aacggcgtca gcgcgggggc aggaacggaa
     5221 ccagcatcgg aatacgccgg cagtaagatc cataggcgtc cgcaccgagc tcattggtga
     5281 ggaagccttc ttccatgcgc gccttctgcc acatcccgag ggagatcagc acggcgccaa
     5341 gcatcgcggt caccgtcccg acagcgatac ctgtcgccag catgccggcg atgaggccgg
     5401 tgtagatcgg gtggcggaca agcccatacg ggccggtgtc aatgacctga tgaccttcct
     5461 tgcgcgtgat cgagttcgac cagaagcgtc cgaggtgaat tcgcgcccac cacgtgaacg
     5521 agatccccgc cagggtcagg gcgctaagca cgtagatgcc aggattgcca aagtgccaga
     5581 gcgggctttc gcccagtatc tgtgcggtcc agggcgtgaa gagaatggct cccgcaagaa
     5641 tcggaatacg gtaggcttgg gagtcccagg tcatcacctg tttttgcgtg cgacccgacc
     5701 agaatgacgc cacgacccag ctgacaagcc aggtaatcca gatgatcgcc agcaactggg
     5761 tgggccaggt ggcggtccat ccactccagg cgatggcaag tagtgagtgc ggatcaaatg
     5821 acatgtttca gattatacga ccggattgca ttatgagcca tagcgccgac ccgcatgagc
     5881 gggccgaaca cgcggagccc cggcgcgatc aaatcgttgc tgatcgccgg cggcgtcacg
     5941 gagataggcg atggtctcgc gcaacgcggg ctcaaccggc cgcggcgcgt agccaagttc
     6001 gcgttgggct ttttcgatcg aaagggacgt cgcgcgccgc gcgatccgca caccttcgga
     6061 cgttcccgag ggaggctgtc gcgtcacgtg atcggcgatg agctccaata accccgcgaa
     6121 ggtttccgca actttgccgg gaacgggaat gagtaaatga cggcgaccgc tgttttccgt
     6181 caccaggcga agaacctgcc ttagccggat gccttcgccg ccgaaaatgt agcgatggcc
     6241 gacttgcccg cgttccatcg ccagaaccag acccgttgcg acgtcgcgta cgtcgacgag
     6301 gttcacgatg aaatcgagat acaattgaat gcgccggttg agaaaatgcc gaagcatcgc
     6361 gaccggcggc gtgagattat gatcgtgcgg cccgatgggc atggtcggcg tgccgatgac
     6421 gaccggaaaa ccggacgctg cagcttgcat cgcgcgctgc tccgccagca tcttggagcg
     6481 ggtataaggc cccggcatgt ggtcagccat cggcagggtg tgatccgcgc cggcttcctc
     6541 ggaagctgaa gagcggaaaa gaatggattc ggtcgagcag tgcaggaatc gccgagtgcc
     6601 ctgcctgcgg gcagcagcaa ggacaacttc cgtgccgcgg caattgaccg catgaaaatc
     6661 gcttttctgc cgcatccaca tgccgggcag accggcgaga tgatagactt gttcgacgcc
     6721 gcgcaaggct tcccctaccc ggtccggatc cagcaccgag ccttcgacat actcgacttc
     6781 cggctcggga tggctcggag ggtggatatc cagcacgcgc acctgcagac cccgttcgac
     6841 gagcgtcgat accaggtgcc gtccgatgaa tccgctgccg ccggtaatga gtacagaggt
     6901 catgagtttg ggtgcgtctc gccgttatgt gcatcggcgt aaaacgtagt gcccagagcc
     6961 gctgtttgcg gaactggatc gatgatcagc gcaagatcgt gcctaaaccc gagcgcaatg
     7021 aataacttcg ccatcacaaa catcggaccg agcaatagat ggggcggatt gtccagaagc
     7081 gccggctgtc gtcgttcgaa cagccgatgc ccgataatct gcaaactgac gcccacgaca
     7141 atcagaacag ccgagatcga ccacacggca ccgacactta cgttgctgac gatgatggcc
     7201 gcagtcgaaa gcaataaaac cgcggcgccc acgatcccca tcccgagagc ggtatcgagc
     7261 agcaaccagt aaatcagaac gggcaagacc atcatggcag ccatcgtggt ttggacgcca
     7321 aatacgtgga ccgaccaaaa attgagggga agaatagcgg ccaggaacaa aaatatgatc
     7381 ccgaacacat gcatcacgca gttccaccaa tcgcggtgat actcgacgta atccgcaagc
     7441 tggcgccgaa aataagaatt catactgatt tcgctacaaa tctcgcttgc gggaacatcc
     7501 cgcagcccag gtcacaggaa tcaggtgggt ccccacatca cctggttcct tggccggcac
     7561 cttaagcttg ggatcgggcc cagtcaaagg acagcccggc ttttgttgtt cgcagatcgg
     7621 ggcaagacca cgacaggcaa atgacaattg cgcccgtaat caggttgacg ctcagtaact
     7681 tatccgttgt cctgcattgg gatgataccc ggcagagccc gccgtcgtcg atctttattc
     7741 cgctgccgcg acatgtggtc gctcgacgtt ctcgatctcc gcttgcggtt cgagcctgcg
     7801 cttcaggcgg cggtcgatcc ggtcgagata aatatagatc accggcgtga tgaacagcgt
     7861 caaaagctgc gaaacgcaga gaccgccgac cacggcgatg ccgagtggct ggcgcaattc
     7921 agcaccagcg ccggcgccaa tcgcaatcgg cagcgtgccg aaaatcgccg cgaatgtcgt
     7981 catcatgatc ggccggaacc gcagcagtgc ggcctcccgg atggcatgct cagcgctcaa
     8041 gcccacacgg cgacgctcca gcgcgaaatc caccatcatg atggcgttct tcttgacgat
     8101 gccgaccagc atcacgatac cgatcatcgc gataaccgag agttccatgt tgaacagcat
     8161 cagcgtcagg atcgcgccga tgcccgccga cggcaggccg gaaatgatcg tgatggggtg
     8221 aatgaagctc tcgtagagaa tgccgaggat cacgaaggca gcaaacaccg ccgccaggat
     8281 cagcacgccc tgcccgcgca acgaatcctg gaacacctga gccgtaccgg agaacccggt
     8341 ggcgatggtt gcgggaagat tcgaactctg ctcgatcgac gtgatctggt ccaccgcgta
     8401 acccagcgag tagttcggtg cgaggttgaa cgagatcgtc accgctggct gctgaccctg
     8461 gtggttgatc agcagcggtc ccaccgtcgg cgccagtttg gccaccgcat cgagcgggat
     8521 ggtctggtta tt
//
LOCUS       DATNOI010000356         6125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1864254,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000356 DATNOI010000000
VERSION     DATNOI010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6125
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1864254"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /gene="sucA"
                     /locus_tag="VN968_14280"
     CDS             <1..898
                     /gene="sucA"
                     /locus_tag="VN968_14280"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609293.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HXL30439.1"
                     /translation="GDQGHYEVINSALSEEAVLGFEYGYSLAEPKALAIWEAQFGDFA
                     NGAQVLFDQFISSGERKWLRMSGLVCMLPHGYEGQGPEHSSARLERFLQMCAEDNMQV
                     VYATTPANFFHVLRRQLHREIRKPLILMTPKSLLRHKRAVSRLDELGADTTFHRLLYD
                     DAAMLPDEKIKLVSDDKIRRVVLCSGKVYYDLYEEREKRGIDDVYIMRVEQLYPVPLK
                     ALVQELGHFKRAELVWCQEEPRNMGAWHFMEPYLEWVLNQVNAPNKRPRYAGRAASAA
                     TATGLMSKHLAQLKALLDEALS"
     gene            1039..2292
                     /gene="odhB"
                     /locus_tag="VN968_14285"
     CDS             1039..2292
                     /gene="odhB"
                     /locus_tag="VN968_14285"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HXL30440.1"
                     /translation="MTEIRVPTLGESVTEATIGRWFKKAGDAVAVDEPLVELETDKVT
                     IEVPAPSAGTLGDIIAKDGETVAVGALLGQINDGAAAAAGKPAAAPARAAAPPPAAAA
                     SAAASATTTQRVPAADAPLAPSVRKLSAESGLDAATVPGSGKDGRVTKGDMLAAIEKA
                     ASAPTPINQPAAAVQVRAPSPADDAAREERVKMTRLRQTIARRLKDVQNTAAMLTTFN
                     EVDMSHIMAMRAQYKDVFEKKHGSKLGFMGFFSKACVQALKDIPAANAEIDGTDLIYK
                     NYYHIGVAVGTDKGLVVPVVRDCDHKSIAEIEKSIADFGRRARDGQLKIDEMQGGTFT
                     ITNGGIYGSLMSTPILNAPQSAILGMHKIQDRPVAIAGKVEIRPMMYLALSYDHRVID
                     GKEAVTFLVRVKESLEDPARLVLDL"
     gene            2305..2532
                     /locus_tag="VN968_14290"
     CDS             2305..2532
                     /locus_tag="VN968_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL30441.1"
                     /translation="MAFGPNPPPIQAHICVKDGLEAIAYVDTAVDRARSAGAEIVMSA
                     QDTFWGARYGRVRDPFGHVWAFNAPLKENAA"
     gene            2573..3319
                     /locus_tag="VN968_14295"
     CDS             2573..3319
                     /locus_tag="VN968_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL30442.1"
                     /translation="MTNRVAVITGGSRGIGRATAIAAAARGFKVCVGYASNEAAAVSV
                     VSAIEANNGKAIAVKCDVGNESDILALFTAADKFGTLGALINNAGIVGPTSRVDEMSA
                     ERIQRMMAVNITGSILCAREAVKRMSTRHGGQGGVIVNLSSVASKLGSPNTYVDYAAS
                     KGAIDSFTIGLGYEVAGEGIRVAAIRPGLIDTEIHASGGEPDRAHRLSHMVPMKRVGT
                     ADEIASAIVWLMSDEASYVTSAILDVSGGR"
     gene            3374..4771
                     /gene="lpdA"
                     /locus_tag="VN968_14300"
     CDS             3374..4771
                     /gene="lpdA"
                     /locus_tag="VN968_14300"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXL30443.1"
                     /translation="MATYDLVVIGTGPGGYVCAIRAAQLGMKVAVVEKNATLGGTCLN
                     VGCMPSKALLYASEMFEEAGHSFAKMGVSVSAPKLDLPSMMNFKQQGIDGNVKGVEFL
                     MKKNKIDVISGKGKILGTGKVEVSGSETRIIETKNIVIATGSDIARLKGIEIDEKRVV
                     SSTGALSLDQVPEKLLIVGAGVIGLELGSVWHRLGAKVTVVEFLDRILPGMDGEVAKQ
                     FQRILEKQGFAIKLSTKVTGIDTTGNTLVAKIEPAAGGASETLQADVVLVCIGRVPYT
                     EGLGLKEAGVALDNRGRVQIDPHFATSVKGVYAIGDVVAGPMLAHKAEDEGVAVAEIL
                     AGQAGHVNYDVIPGVVYTTPEVSSVGKTEEELKQAGVAYTSGKFPFTANGRSKVNQTT
                     DGFVKVLADAKTDRVLGVHIVGREAGEMIQEAAVLMEFGGSAEDLARTCHAHPTRSEA
                     IKEAALAVGKRTIHM"
     gene            4810..5346
                     /locus_tag="VN968_14305"
     CDS             4810..5346
                     /locus_tag="VN968_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30444.1"
                     /translation="MMRRLLQPFWVLLAVIFLIEAWLWDHLEPIVARVVALIPLRAFK
                     QWLAERVDTLSPAMTLIVFFVPVIPLFPLKLVGFWLLTHEYWFSAITTIIFAKFLGVG
                     VAAFVFDVTRHKLLEMAWFETLYEFVIALRAKAAALVDPIKLRIMEALRGGGGGGGWS
                     SRMLRLIQRFRKSVHEAR"
     gene            complement(5524..6114)
                     /locus_tag="VN968_14310"
     CDS             complement(5524..6114)
                     /locus_tag="VN968_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HXL30445.1"
                     /translation="MSAHESMEQAEHTKEAAGENRKIALLIAVIALCLALSETLGKGA
                     QTESISKNVEASNLWAFFQAKTIRRTMVQATSDQAKLSLGVMGDAAAKAALQKQIDEW
                     QKTAARYRSEPETGEGSEQLSERAKHAEEERDLATAKYHHFELASAAFQIGIVLASAT
                     IITGILALAWISGLLALAGIAFTALGLFVPHLLHLP"
BASE COUNT         1174 a         1868 c         1941 g         1142 t
ORIGIN      
        1 cggcgatcag ggccattacg aagtcatcaa ctcggcgctg tcggaggaag ccgtgctcgg
       61 cttcgagtac ggctattcgc tggcggaacc gaaggcgctg gcgatctggg aagcgcagtt
      121 cggcgacttc gccaacggcg cccaggtgct gttcgaccag ttcatttcct cgggcgagcg
      181 caaatggctg cgcatgtccg gcctcgtctg catgctgccg catggctatg aggggcaggg
      241 accggaacat tcctccgcgc ggctcgagcg cttcctgcag atgtgcgccg aggacaacat
      301 gcaggtggtc tatgccacca cgcccgccaa cttcttccac gtgctgcggc gccaattgca
      361 tcgcgagatt cgcaaacccc tgatcctgat gacgccgaag tcgctgctgc ggcacaagcg
      421 cgcggtttcc agactggatg agctcggcgc cgacaccacg ttccatcgcc tgctctacga
      481 tgatgcggcg atgctgccgg acgaaaagat caagctggtt tccgacgaca agatccgccg
      541 cgtcgtactg tgctcgggca aggtctatta cgacctctat gaggagcgtg aaaagcgcgg
      601 catcgacgat gtctatatca tgcgcgtcga gcagctctat ccggtgccgc taaaggcgct
      661 ggtgcaggaa ctcgggcact tcaagcgtgc cgaactggtc tggtgccagg aagagccgcg
      721 caacatgggc gcgtggcatt tcatggagcc ctatctcgag tgggtgctga accaggtcaa
      781 cgcgccgaac aagcgtccgc gttacgccgg acgcgccgcg tccgcggcga ccgccaccgg
      841 cctgatgtcc aagcatctgg cgcagctcaa ggcactgctc gacgaggcgt tgagctgaga
      901 aaattgcaat ccgtcatgcc ccgcgaaagc ggggcatcca gtaacccgcg cggtctacct
      961 cgatcatgat cgccacggat tactggatcg ttcgctttca cggacgatga ccagacaggg
     1021 tatctgggga accaaccaat gactgaaatt cgcgttccga cgctcggcga atccgtcacc
     1081 gaggccacta tcggccgctg gttcaagaag gccggcgatg ccgtggcggt cgatgagccc
     1141 ttggtcgagc tcgagaccga caaggtcacc atcgaagtcc cggcgccgtc ggccggcaca
     1201 ctcggcgaca tcattgccaa ggacggcgag accgtcgccg tcggtgcgtt gctcggacag
     1261 atcaatgacg gcgcggcggc agcagccggc aaaccagctg ctgcgccggc cagggccgcg
     1321 gcgccaccgc cagctgccgc cgcgtccgcc gccgcatcgg ccaccacgac tcaaagagtt
     1381 ccggcggctg atgcgccgtt ggcgccgtcg gtgcgcaagc tctccgccga aagcgggctc
     1441 gatgccgcca ccgtccccgg ctcgggcaag gatggccgcg tcaccaaggg cgacatgctc
     1501 gccgcgatcg aaaaggcagc gtcggcgccg acgccgatca atcagcccgc cgccgcggtg
     1561 caggtgcgcg cgccttcgcc cgccgacgat gccgcgcgcg aagagcgcgt gaagatgacg
     1621 cggctgcgcc agaccattgc gcgccgcctg aaagacgttc agaacaccgc cgcgatgctc
     1681 acgaccttca acgaggtcga catgagccac atcatggcga tgcgggccca gtacaaggac
     1741 gtgttcgaga agaagcacgg cagcaagctg ggattcatgg gcttcttcag caaggcctgc
     1801 gtgcaggcgc tgaaggacat accggcggcc aatgccgaga tcgacggcac ggatctgatc
     1861 tacaagaatt actatcacat cggcgtcgcg gtcggtaccg acaagggcct cgttgtgccg
     1921 gtggtgcgcg actgcgacca caagtcgatc gccgagatcg aaaagagcat cgccgatttc
     1981 ggacggcgcg cgcgggacgg ccagctcaag atcgacgaga tgcagggcgg cactttcacc
     2041 atcacgaacg gcggcatcta tggttcgctg atgtcgacgc cgatcctgaa cgcaccgcaa
     2101 tccgcgatcc tgggcatgca caagattcag gatcgcccgg tcgcgatcgc cggcaaagtc
     2161 gagattcgtc cgatgatgta tctggcgctg tcctacgatc accgtgtcat cgacggcaag
     2221 gaagcggtga cgttcctggt tcgcgtcaag gaaagcctgg aagatccggc gcggctggtg
     2281 ctggatctgt aacggagcgc ggccatggcg ttcggaccca atcccccgcc aatccaggcg
     2341 catatttgcg tcaaggacgg acttgaggcg atcgcttatg tcgatacggc agtcgaccgc
     2401 gctcgatcgg cgggcgccga gatcgtgatg agcgcgcagg atacgttctg gggtgctcgt
     2461 tatggccgcg tgcgcgatcc tttcggccat gtctgggcgt tcaacgcacc gctaaaggag
     2521 aatgcggctt gatcaatcat gcgatcatcg ttcgcggcac acgggggcaa atttgacaaa
     2581 cagggttgca gtgatcaccg gcgggagccg cggcatcggc cgcgccaccg cgattgccgc
     2641 cgccgcgcgc ggcttcaagg tttgcgtcgg ctacgccagc aatgaagccg ccgccgtcag
     2701 cgtcgtctcc gccatcgagg cgaacaacgg caaggcgatc gcggtgaaat gcgatgtcgg
     2761 caacgagagc gatatcctcg cgctgttcac ggccgccgac aagttcggaa cgctcggtgc
     2821 gctcatcaat aatgcgggca tcgttgggcc gacatcgcgg gttgacgaga tgtccgccga
     2881 gcgcattcag cgcatgatgg ccgtcaacat caccggcagc attttgtgcg cgcgcgaagc
     2941 ggtgaagcgg atgtccacgc gccacggcgg ccagggcggc gtcatcgtca acctgtcgtc
     3001 ggttgcctcg aaactcggct cgcccaatac ctatgtcgat tacgcggcct ccaagggcgc
     3061 catcgattcg ttcacgatcg gccttggcta cgaagtcgct ggcgagggca ttcgcgtcgc
     3121 cgcgatccgg ccggggctga tcgataccga gattcacgct agcggcggcg agcctgaccg
     3181 cgcgcatcga ttgagccaca tggtgccgat gaagcgggtc ggcaccgccg atgaaatcgc
     3241 cagcgccatc gtctggctga tgtcggacga ggcctcctac gtgaccagcg ccatacttga
     3301 tgtatcgggc gggcgctaat acccgtcaca gtttctagct acaggactcc catttagcta
     3361 caggactccc atcatggcta cctacgatct cgttgtcatc ggcaccggcc ccggtggcta
     3421 cgtctgtgcg atccgcgcgg cgcaactcgg catgaaggtc gccgtggtcg agaagaacgc
     3481 aacgctcggc ggtacctgcc tcaatgtcgg ctgcatgccg tcaaaggcac tgttgtacgc
     3541 ttccgagatg ttcgaggagg ccgggcattc gtttgccaaa atgggtgtca gtgtctccgc
     3601 gccaaaactc gatctgccct cgatgatgaa tttcaagcag cagggcatcg acggtaacgt
     3661 caagggcgtc gagtttctga tgaagaaaaa caagatcgac gtcatcagcg gcaagggcaa
     3721 aatcctcggc accggcaagg tcgaggtatc aggcagcgag acgcggatca tcgaaaccaa
     3781 aaacatcgtc attgccaccg gctccgatat cgcgcggctg aagggcatcg agatcgacga
     3841 gaagcgcgtc gtgtcgtcga ccggcgcgct gtcgctcgac caggtgccgg agaaattatt
     3901 gatcgtcggc gccggcgtga tcgggctcga actcggctcg gtgtggcacc ggctcggcgc
     3961 aaaggtcacc gtggtggagt ttctcgaccg catcctgcca ggcatggatg gcgaagtcgc
     4021 caaacaattc cagcgcattc ttgaaaaaca gggctttgcg atcaagctga gcaccaaggt
     4081 caccggcatt gacaccacag gcaacacgct tgtcgcgaag atcgagccgg ccgcgggcgg
     4141 cgcgtctgag acactgcaag ccgacgtggt gctggtctgc atcggccgcg tgccctacac
     4201 cgagggcctc ggcctgaagg aagccggcgt cgcgctcgac aatcgcggcc gtgtgcagat
     4261 cgatccgcat ttcgcgacca gcgtcaaggg cgtgtacgcg atcggcgatg tggtggcggg
     4321 cccgatgctc gcccacaagg ccgaggacga aggcgtcgcg gttgcggaaa ttctcgcagg
     4381 tcaggccggg catgtgaact acgacgttat cccaggtgtt gtgtatacca cgccggaagt
     4441 gtcgtcggtc ggcaagaccg aggaagagtt gaagcaggcg ggcgtggcct acacatcagg
     4501 caaattccct ttcaccgcca acggccgctc caaggtcaat caaaccactg acggattcgt
     4561 gaaggttctc gcagatgcga agaccgatag ggtgcttggc gtgcacattg tcggccgcga
     4621 ggccggcgaa atgatccaag aagccgcggt gctgatggag tttggcgggt ccgccgagga
     4681 tctggcgcgc acctgtcatg cccatccgac ccgttcagag gccatcaagg aagctgcgct
     4741 tgcagtcggc aagcgcacga tccatatgtg attcacgctg cggcttagtg tcgggcgggg
     4801 ataacattga tgatgcgccg ccttctacag ccgttctggg ttctgcttgc ggtcatcttc
     4861 ctgatcgagg cctggctgtg ggatcacctc gagccgatcg tggcgcgggt ggtggcgctg
     4921 attccgctac gcgcgttcaa gcagtggctc gccgagcgcg tcgatacgct gtcgccggcg
     4981 atgacgctga tcgttttctt cgtgccagtc attccgctgt ttccgctgaa gctggtcggc
     5041 ttctggctgc tcacccacga gtattggttc agcgcgatca cgactatcat ctttgcaaaa
     5101 ttccttggcg ttggcgtcgc ggccttcgtc ttcgacgtca cccgccataa attgctggag
     5161 atggcgtggt tcgaaacgct ctatgaattc gtgattgcgt tgcgcgcaaa agccgccgca
     5221 ctggtcgatc ccatcaaatt gcggatcatg gaagccctgc gtggtggagg cggtggcggc
     5281 ggctggtcgt cgcggatgct gcggctgatc cagcgctttc gcaagagcgt gcacgaagcg
     5341 cgctgaattc gaagacactc gcaagctatc tcgtcatccg aaggtgtgcg ctctctctct
     5401 ctctctctct ctcagtccaa ctcgggttac ccgagttgga caaaatcaca atgtcgaagt
     5461 cggatatatc cgacttcgac tgggagaggg ttgagatgag gggttgcgga gaagtgagtc
     5521 aagctagggc aaatgcagca gatgcggcac gaacaggccg agcgcggtga atgcgatacc
     5581 tgccaaagcc agcaggcccg agatccaggc cagtgcaagt atgccggtta taatcgtcgc
     5641 cgaagccagc acgatgccga tctgaaaggc ggcggaggcg agttcgaagt gatgatactt
     5701 tgccgtcgcc aggtcgcgct cttcctcggc gtgcttggcg cgctcggaca attgttccga
     5761 cccttcgccg gtttccggct ctgagcggta gcgcgccgct gtcttctgcc actcgtcgat
     5821 ctgcttctgc agtgccgcct tggcggcagc gtcgcccatg acgccgaggc ttagtttggc
     5881 ttggtccgat gtcgcctgca ccatcgtgcg gcggatcgtc ttggcctgaa agaacgccca
     5941 gagattcgag gcttcgacgt tcttgctgat cgactcggtc tgcgcgccct tgcccagcgt
     6001 ctccgacagc gcaaggcaaa gtgcgatcac ggcgatcagc agcgcgatct tcctgttctc
     6061 gccggcggct tccttggtgt gctccgcctg ctccatgctt tcatgtgcgc tcatgattcc
     6121 ctccc
//
LOCUS       DATNOI010000357         6560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1433485,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000357 DATNOI010000000
VERSION     DATNOI010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433485"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..446
                     /locus_tag="VN968_14315"
     CDS             240..446
                     /locus_tag="VN968_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30446.1"
                     /translation="MESISDGHLLSRILLTLAAIGYGFVTIKADFNRTHATNPLWTPH
                     ARFHVVWQVLSYTGSLSSPSVSFG"
     gene            998..1846
                     /locus_tag="VN968_14320"
     CDS             998..1846
                     /locus_tag="VN968_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL30447.1"
                     /translation="MRQRQSSAIILVGKSILLREGLARILRSANFRILASVSCADDLL
                     PSKLQLHQPLFLVIHTGDDCDAAVEQIKQFRNQHPGGRIAIVADHYRLSELVSAFRAG
                     ANGYFVDVMACDVFIKSIELLMMGETIFPPAFLSFVLDSEGDRLGQAAPRDENNQETL
                     IRTENTLAPQLSTREKSILRCLVEGDSNKCIARKIDITEATVKVHVKAILRKIGVQNR
                     TQAAIWGMNNGSATRLANNSSPPSTFDVSKQLPNPVEVISEIKQIGTSVPLGAIGHDA
                     NHVKRV"
     gene            2128..2988
                     /locus_tag="VN968_14325"
     CDS             2128..2988
                     /locus_tag="VN968_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30448.1"
                     /translation="MRTLLIGALAATLVGCSCLLPPQASIEGCTDANGFACFDRMAAS
                     QPIELKPASFKTESATTEIKSTIAAKTQKPSSARARDRAHLAMKTAKSTAAADSDTTR
                     AGIADSDPRGGAVANSNTRTIQEQLAANTAVAEQVTAATPVPAPEQKANNTNRSGHAE
                     TLLRDDAEKTAAASPNNRDHLVALLIARPEIKSVSDLTSKIVAIDDRHSASNDSVRTA
                     IAAAGATEVQLSEGQTKAINRLISGEVPAAVLALVSAEAAEGFPEVAGFKIFRIPLSP
                     RSLKARRDTP"
     gene            3275..3484
                     /locus_tag="VN968_14330"
     CDS             3275..3484
                     /locus_tag="VN968_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HXL30449.1"
                     /translation="MCISARDQIKGTVVEVIKGATTSHIRIDIGGGVIVTASITNEAA
                     DDLKLTKGKAATAVIKASDVMVGVD"
     gene            3481..4503
                     /locus_tag="VN968_14335"
     CDS             3481..4503
                     /locus_tag="VN968_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HXL30450.1"
                     /translation="MIDRRALLAGLSAALVAPRSASAATVNDAAGRAVPVPIKVARVF
                     PAGPPAAILLYTLAPDLLIGWPRANRPEECAYMLPEICTRPEVGRITGRGNTANLETV
                     LALKPDLILDVGSTSATFVSLADRVQEQTRIPYALLDGRFAGIAQTYRSLGALIGRPE
                     DADKLARYADDTLKTIRGRIEPIATAARPRVYYARGPRGLATGLGGSINVETIELISR
                     NVAGETQGGLANVSIEQILVWDPEVIVTIDQEFAATVRNDPSWASVKAVRDNRVHLSP
                     KMPFGWVDFPPSVNRLIGLWWLAKILYPERFPEDLRALTQDFYTRFYHVTPSSAQIDH
                     VLAGRD"
     gene            4517..5536
                     /locus_tag="VN968_14340"
     CDS             4517..5536
                     /locus_tag="VN968_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXL30451.1"
                     /translation="MRNARSAVPGFAIAVAVLIAGLLLALTVGRYPGGLGDLLNVLYA
                     KAAGHRADVAPAVESVILQVRGPRVIAAMLVGAALAVAGTAFQGLFRNPLVSPDILGA
                     SSGAALGAVIGIYLSLGVFAIQAVAFVGGLIAVGVVYVIGASVRSRDPILVLVLTGVV
                     VGSLFGAGVGLVKYLADPYNQLPAMTFWLLGSLSATGVADLLPLFGPVALGAAVLIAL
                     RWRMNVMSLPEEEARALGVATGPLRIAIVAAATLVTSASVATAGIIGWVGLVVPHLAR
                     SLVGPDFARLLPAAAILGGGYLLLIDTLARTMAQVEIPLGILTAVIGTPFFIWLLASV
                     QKNWS"
     gene            5533..6294
                     /locus_tag="VN968_14345"
     CDS             5533..6294
                     /locus_tag="VN968_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30452.1"
                     /translation="MILEGHQLTIGYQDRVVGAGLDVKLQNGEVLALLGPNGGGKTTL
                     LKTLLGLLAPKAGEVRIDGAALSSRTVRERARLIAYVPQTHVATFAFSVEAVVLMGRT
                     AHGDLFARPTSDDRRVAASMLDRFGIAALAQRPYTMISGGERQLVLLARALAQEPQFI
                     VLDEPTANLDFGNQGKVMREIRELAASGLGVLFTTHDPNHALRAADRAYLLRGGERIA
                     EGPVGEILTRERLEALYGAPVHMITGAVGTAFIPG"
BASE COUNT         1304 a         1994 c         1961 g         1301 t
ORIGIN      
        1 gctacgcctg acgctgacga aaagagcgcg gcgatccgtc atttccggaa ataacgacgc
       61 agcgaggtca tgtccgcttt agctcgatga tcggacatcg ccgaccataa gggccaagtc
      121 cgaaaggtgc caacagcgga agtagccatc ccggtcttaa ttgtgattgg atacagccgg
      181 aatgatcgac gggcacgaag atcacaattc tcgatttaga ttcaacagtg aggctaccga
      241 tggaaagcat tagcgacgga catttgctat ccagaattct tttgacgctc gccgccatcg
      301 gctacggctt cgtgacgatc aaagccgatt tcaatcgcac ccatgcaact aatccactat
      361 ggacgccgca cgcacgcttc cacgtcgtgt ggcaggtact gagctatacc ggctcgctgt
      421 catcgccctc tgtctcattt ggctgaaagg accgcttccg aaagagagac tcgctgggcg
      481 ccgcagttta tggggctttt tttcgccacg ctgtttgtac gaaagctgtt tggcggcgca
      541 ctctatgatc aaaacggcta cccgcccttt cgatcgccgg taggaccgtg gttatgggat
      601 gcgaacgtca ccgcgttcag tgttttgtcg acgatcttgg cggttggaat tctggtagtg
      661 ggaggcgcgc tttacaccgc tgcccagcaa ttccccggcg cacccacctg tataggtata
      721 tagggatatc gaggatatcg atctaatatc atgaaggctt cgtaaagctt catggaaaca
      781 agcgcggagc tttcaaattt gaccacgaac catttcctat gaaaggggag agccccaccg
      841 actgaagaag ccaaaatatt cgacaccaag acttccaccg aagaacaaca cttccaccga
      901 agaagcgctc gttaaccggt cgacaagtca caaaatgaga gggcctgagt tcaatgggcc
      961 ggtcgagtat caaggcgtgc atgctgagag ggtcaccatg cgccagcgac aatcttctgc
     1021 aatcatactc gttgggaaaa gcattttact cagagaagga cttgccagaa tcctccgatc
     1081 ggcaaatttt cgtatccttg cctcggtatc atgcgccgat gatttgctcc cgagcaaact
     1141 ccaactacac caaccgctat ttcttgtgat ccataccggc gatgattgtg acgccgcggt
     1201 ggaacaaatc aaacagttta ggaaccagca ccctggcggg cgcattgcca tcgtggccga
     1261 tcactatcgg ctatccgaac tggtttcagc atttcgggca ggcgccaacg gctatttcgt
     1321 cgacgtcatg gcctgcgatg tattcatcaa atccattgaa ctgctgatga tgggtgaaac
     1381 aattttcccg ccggcatttc tgtcattcgt tctcgattcc gaaggcgacc gcctcggcca
     1441 ggccgcgccg cgcgacgaaa acaaccagga gaccctcatc aggaccgaga acactctcgc
     1501 cccacagctt tccacgcggg agaaatcaat cctgcgctgc ctggtcgaag gcgactccaa
     1561 caaatgtata gcgcgaaaga tcgatatcac cgaggcgacc gtgaaggtcc acgtcaaggc
     1621 gatccttcgc aagatcgggg tccagaaccg gacacaggcg gcgatttggg ggatgaacaa
     1681 cgggtcagcg acacgactgg caaacaacag ctccccgccc tcaaccttcg atgtgagcaa
     1741 gcagcttcca aatccagtcg aggtgatctc cgagatcaag caaatcggga catcggtacc
     1801 gctgggtgcg atcggacacg atgcaaatca tgttaagcgc gtctaattgc tcagggatta
     1861 gcagctccga tgataacgat gcggcccagc ccgatggtac tttcgtcgct cgtaagcatt
     1921 gcggaaacgc ttcgaccgcc agtgcaacgc tgctcaccta gcgcgaaaac tgcgttgcgc
     1981 caaccttaac catgcaagac cttctcgcgg gcgaagtccg cacgcttaat gagcgtttaa
     2041 catttttgca caacagatag acgatcagcg ccaacactat cacgcagcaa gaattgcgag
     2101 cggaactcac cctctaaggg ggattgcatg aggacgcttc tcatcggcgc cctggccgcg
     2161 accttggtcg gctgcagctg ccttttaccg ccgcaagcga gcatcgaagg atgcacggac
     2221 gcgaacgggt tcgcctgttt cgacaggatg gccgccagtc agccgattga actaaagcca
     2281 gcgtcattca agaccgaatc cgcgacaacg gaaatcaagt ccacgatcgc ggcgaagacc
     2341 caaaagccgt cgtctgctcg cgcccgcgat agggcccatc tcgccatgaa aacggcgaag
     2401 tccaccgccg ctgccgattc cgatacgacg cgagcaggca tcgcggattc agatccccga
     2461 ggcggtgccg ttgcgaattc caataccaga acgatacagg agcagctggc ggccaatacg
     2521 gcggtcgcgg agcaagtgac ggccgccacg ccggtacccg cgccggaaca gaaagcgaat
     2581 aataccaacc gttccggtca cgcggaaact ctgctgcgtg acgatgccga aaagaccgca
     2641 gctgcgtcgc caaacaacag ggaccatctg gttgcccttc tcatagctcg tccagaaatc
     2701 aagtcggtat ccgatctaac cagtaaaatc gttgcgatag atgacaggca ttctgcgtct
     2761 aacgatagtg tccggaccgc gatcgctgca gcgggggcga ctgaagtcca actgagcgag
     2821 ggtcagacga aggcgatcaa tcggttgatc agtggagagg tgccggctgc ggttctggct
     2881 ttggtgtctg cggaagcggc ggagggattt cccgaagtcg caggcttcaa gatatttcga
     2941 atcccgctct cgcctcgttc attgaaggca cgacgggata caccatgaca aatcggccat
     3001 tcgccagttg tatgcgcgcc gtgatcgatg cgcgaggcaa agtgacgatc ccgatctgag
     3061 cgccagaccc aatgtccgtt ttgggacccg gaacggacat cgaccgcttg tttgatcttt
     3121 cacatctcgt ggaggcgcgc tactgtctgt tggatagcgg cgcgccgtaa gcgcgatgca
     3181 tcttttgggg acggcggtcc cgatttttcc attccagaga ccagccaaca ggtgcttgcc
     3241 attcgcagcg ccagccttac cctgtgagga ccctatgtgc attagtgcac gcgaccaaat
     3301 caaaggcacc gtcgtcgaag tcataaaggg cgccaccacg tcccatatcc ggatcgacat
     3361 tggcggcggg gtcatcgtca ccgcgtcgat taccaatgaa gcggcggatg acctcaaatt
     3421 gacgaaaggt aaggcagcca ccgccgtgat caaggcgagc gacgtgatgg taggcgtcga
     3481 ttgatcgacc gtcgcgcttt actcgcaggc ctgtcagcgg cgctggtcgc gccgcgctct
     3541 gcatccgcgg ccaccgtgaa cgatgccgcg gggcgggccg tgccggttcc gataaaggtt
     3601 gcgcgcgtgt ttcccgccgg accgccggcg gcgatcctgc tctatacgct ggcgccggac
     3661 ttgctgatcg gctggccgcg cgccaaccgg ccggaagaat gcgcctacat gctgccggag
     3721 atttgcaccc ggccggaagt cgggcgcatt accggacgcg gcaataccgc caacctggaa
     3781 acagtgctcg cgctcaaacc cgatctcatt ctcgacgtcg gatcgaccag cgcgaccttc
     3841 gtatcgctgg cggatcgggt gcaggagcaa accagaattc cctacgccct gctggatggc
     3901 cgcttcgccg ggatcgccca gacctaccgt tcgctcggcg cgttgatcgg tcgcccggag
     3961 gatgccgaca agcttgcgcg ctatgccgac gacacgctca agactattcg cggccgcatt
     4021 gaaccgatcg caacggctgc gcggccgcgg gtctattatg cgcgcgggcc gcgcgggctc
     4081 gcgactggcc tgggcggctc gatcaacgtc gagaccatcg aactgatcag tcgcaacgtt
     4141 gcaggcgaaa cccagggcgg cctcgccaat gtgtcgatcg agcagattct ggtctgggat
     4201 cccgaagtga tcgtgaccat cgaccaggag ttcgcggcga cggtgcggaa cgacccttcc
     4261 tgggcttcgg tcaaggccgt gcgcgacaac cgggtgcatc tgtcgcccaa aatgccgttc
     4321 ggctgggtgg attttccacc atcggtgaac cgcctgatcg ggctgtggtg gctagcgaaa
     4381 attctttatc cggaacgctt tccggaagat ctgcgcgcgc tgacgcagga cttctatacc
     4441 cgtttctatc acgtcacccc gtcatcggcg caaatcgacc atgtacttgc aggccgggat
     4501 tgatagcctc gcccgcatga gaaatgcccg atccgccgtg cccggatttg ccatcgcggt
     4561 cgcggtgctg atcgcaggcc tgctgctcgc gcttaccgtt ggccggtatc cgggcggact
     4621 tggcgacctc ctcaacgttc tctatgcgaa ggcggccggc caccgcgccg acgtcgcgcc
     4681 ggcggtcgaa agcgttatcc tgcaggttcg cggtccgcgc gtgatagcgg cgatgctggt
     4741 tggtgccgcg ctcgcggtcg ccggcaccgc ctttcagggg ctgtttcgca atccgctggt
     4801 ctctcccgac attctcgggg cttcatcggg ggcggcgctc ggcgccgtga tcggcatcta
     4861 tctctcgctc ggcgtgttcg cgatccaggc ggtcgccttc gtcggcggcc tgatcgcggt
     4921 cggggtcgtc tatgtgatcg gcgcttcggt gcgctcgcgc gatccgatcc tggtgctggt
     4981 attgaccggc gtcgtggtcg gatcgttgtt cggcgccggc gttggcctcg tgaagtatct
     5041 cgccgatccc tacaaccagc ttccggccat gacgttctgg ctgctcggca gcctgtccgc
     5101 gaccggggtt gccgacctgc tgccgttatt cggtccggtt gcgctcggtg ccgccgtgct
     5161 gatcgccttg cgctggcgta tgaacgtgat gtcgcttccc gaagaggaag cgcgcgcact
     5221 cggcgtcgcc accggaccgt tgcgcatcgc catcgtcgca gccgcgaccc tggtcacctc
     5281 ggccagcgtc gccaccgccg ggatcatcgg ctgggtcggg ctggtcgttc cgcatctggc
     5341 gcgatcgctg gtcggcccgg attttgcgcg gctattgccg gcggccgcga tactcggcgg
     5401 cggctatctg ttgctgatcg acacgctggc gcgcaccatg gcgcaggtcg aaattccgct
     5461 cggcatcctg accgccgtga tcggcacgcc gttcttcatc tggctgctcg ccagcgtgca
     5521 aaagaactgg tcatgatcct cgagggccat caactcacca tcggctatca ggaccgcgtg
     5581 gtcggcgccg ggctcgacgt caaactgcag aacggcgagg tgctggcgct gctggggccg
     5641 aatggcggtg gcaagaccac gctgctcaag accctgctcg gactgctcgc gccgaaagcg
     5701 ggcgaggtgc ggatcgacgg cgcggcgctg tcgtcccgca cggtccggga acgcgcgcgg
     5761 ctgatcgcct atgtgccgca gacccatgtc gcgacattcg ccttttccgt ggaagcggtg
     5821 gtgctgatgg gccgcacagc gcatggcgat ctgttcgccc gcccgacatc tgatgaccga
     5881 agggtcgcag catccatgct cgatcggttc ggcatcgcag cgctggcgca gcggccctac
     5941 accatgatct ccggcggcga gcgccagctg gtgctgctgg cgcgcgcgct ggcgcaggag
     6001 ccacagttca tcgtgctgga cgagccaacc gccaatctcg acttcggcaa tcaaggtaag
     6061 gtgatgcgcg agatccggga actggccgct tccggcctcg gcgtactctt caccacccac
     6121 gatcccaacc atgcgctgcg cgccgccgac cgcgcctatc tgctgcgcgg cggcgaacgc
     6181 atcgccgagg ggccggtcgg agaaattctg acgcgggagc ggctggaggc gctgtatggc
     6241 gcgccggtgc acatgattac cggggctgtt ggcacggcat tcattccggg ttagcgattt
     6301 ccggttttgg cacttttgag acatgcccag atgtccgcag ttcggtcgct ttcgggggaa
     6361 gagcagacgt aggatggaca tcgcgtttct gtagcgattg acccaaatgt atggtccgcc
     6421 cgtgcgttgc aagaagtttc gtcaacctgg cagatgcggt cttgcatcaa tgtatccggc
     6481 ctctgattgg agcgtgttgt gctccgggcc atcatggata tcagcgcgca tgcgagctga
     6541 ttagcggaca ggcctcgaac
//
LOCUS       DATNOI010000358         5162 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_635081,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000358 DATNOI010000000
VERSION     DATNOI010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635081"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VN968_14350"
     CDS             <1..736
                     /locus_tag="VN968_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HXL30453.1"
                     /translation="WSADYFTTIVAIFGTTISPYLFFWQASQEAEDQRVDVTKQPLID
                     KHYGAQKEFHRIRADTIVGMAFSNLIALSIIVTAAATLHAAGKTDIQTSAQAAEALRP
                     IAGAFAEVIFALGIVGTGLLAIPVLAGATAYAVGEGRQWPVGLARKPKEAVAFYSVLA
                     LSAAIGIALNFTSINPISALYWSAVINGVLAVPVMVLLMIMARRRDVMGRFVVGGPLY
                     WLGWLSTAAMLLSVLAMAVGFFVGSK"
     gene            complement(849..1640)
                     /locus_tag="VN968_14355"
     CDS             complement(849..1640)
                     /locus_tag="VN968_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HXL30454.1"
                     /translation="MTARSSLAALGLAVVLGAAFAVLPARAERLIVSVSNHRVTVTPN
                     YSGEELVLFGSVEKDATTPATRTSYDLVVTVSGPRADMVTRRKERKFGIWINTDYRQF
                     LKVPTYLALFSNRPFDAIASPEVERRQQLGLNNVLLTQRVGPDYADVVPNDAFRSAFV
                     RLRSQHGLYREATSAVTFLTPTLFRTGIPLPAEVPIGTYDVEIKLFSDGVLVTKTETA
                     FEIVKVGFEQFVANTARQNGLVYGLVTAFMALMTGWMASIVFRKD"
     gene            complement(1637..2563)
                     /locus_tag="VN968_14360"
     CDS             complement(1637..2563)
                     /locus_tag="VN968_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXL30455.1"
                     /translation="MQLYLPIADIPVNVFLILAMGAAVGFVSGMFGIGGGFLMTPLLI
                     FVGIAPAVAVASVASHIAASSFSGAISYWRRRAIDPALALVLLSGGTIGTALGVWTFT
                     LLRSLGQLDLLIALSYVILLSTVGGLMFWEGLRAILRARRGVAVPMRRSGSHGWIHGL
                     PLKMRFKRSKIYLSVIPVVVVGLIIGFIGAVMGIGGGFILIPIMIYLLRVPTSTVIGT
                     SMVLTLVTMVFATMLHAVTNHLVDAVLALILMVGGVTGAQFGARAGQKIRGEHLRLLL
                     GLLILAVGIRFAIELVIRPDDLFTIRETGGAG"
     gene            complement(2796..3599)
                     /locus_tag="VN968_14365"
     CDS             complement(2796..3599)
                     /locus_tag="VN968_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30456.1"
                     /translation="MSVDDPHDEGSHFRRSPTGPRVAPPRKPIGQPSVRPRHGNGNKR
                     RFWSAAIFVLLISAVGIRAYRDLSRPEAWAYWKESYLSPTMTSALISSADFDGSGHGR
                     RALAISGEIGVATANWFRDRLDEAHLAAGDAVLLSSPGGDLNRAVIMGEIIRSRGLVT
                     AVGVADASGQVRPAYCVSACVLVYAGGKIRYGVEGSMLGVHQFEAMTPMPDPVASTQR
                     IAGILLSYMTKMGVSSAVVEAMSATKEVRWLGPKEASAMKLITDPIGRP"
     gene            complement(3726..>5162)
                     /locus_tag="VN968_14370"
     CDS             complement(3726..>5162)
                     /locus_tag="VN968_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL30457.1"
                     /translation="EILESHAAQPQAAIVPELPPDHPLEPGTRPSGRVPSPSERIAAS
                     ESAISEITASAREQTSTSNFIAAARRAAQAAAAAPVGDKAARSPFKDAARDKTKETSA
                     VTSKIRALLVGASVVVIVLSTFKMAMTLLDGSSIPALPAMESSSEQAPSAQPPAEDGA
                     KPAAPAPAMPSLTSPTPIGRQSFNSSAPNASDSTASVEIPQATAIPFASPVTGSDITG
                     TIPATLAGPTGRKLTTVQVPPTERLPDAIGGPALRAAALKGDPSAAYEIGLRYAEGKG
                     VAANFDEAAKWYDRSAQAGVVPAIFRLGTLYEKGLSVKKDVDIARRYYMQAAERGNAK
                     AMHNLAVLDADGGSKGANYGTASQWFRKAAERGVADSQFNLGILYARGIGVEQNLAES
                     FKWFSLAAAQGDADAVRKREDIAKRLDPQSLAAAKLAIQTFTPEPQPDDAVNVASPPG
                     GWDSAPAQAGAAKPAAKPVLTKRTAAAH"
BASE COUNT          949 a         1613 c         1697 g          903 t
ORIGIN      
        1 ctggagcgcc gattatttca ccaccatcgt tgccatcttc ggcaccacga tctcgccgta
       61 cctgttcttc tggcaggcgt cccaggaagc cgaagaccaa cgtgttgatg tcaccaagca
      121 gccgctgatc gacaagcact atggcgcgca aaaggaattc catcgcattc gtgccgatac
      181 catcgtcggc atggcgttct caaacttgat cgcgctgtcg atcatcgtca cggcggcggc
      241 gacgctgcac gccgccggca agaccgatat ccagacctcg gcgcaagccg ccgaagcact
      301 gcgtccgatc gcaggggcgt tcgcggaagt catttttgca ctcggcatcg ttggcaccgg
      361 gctgttggcc attccggtgc tcgcgggtgc caccgcttac gccgtcggcg aaggacgcca
      421 atggcccgtg gggctcgcgc gcaagccgaa ggaggcggtg gcgttctatt ccgtgctggc
      481 gctgtcggca gccatcggga tcgcgctcaa tttcacctcg atcaatccga tctcggcgct
      541 gtactggagc gcggtgatca acggcgtgct cgccgtcccg gtgatggtgc tgctgatgat
      601 catggcgcgc cgcagggacg tgatgggccg gtttgtcgtc ggcggtccgc tgtactggct
      661 tggctggctt tcgaccgcgg cgatgttgct cagcgtgctg gcgatggctg ttggattttt
      721 tgtcgggagc aagtaagcca aatactccga cctcatcctg aggaggccgc aacgcggccg
      781 tctcgaagga taggcgacac ggacctcatg gttcgagacg gcgcaagcgc ctcctcacca
      841 tgaggtggct agtccttgcg gaagacgatc gacgccatcc agccggtcat cagcgccatg
      901 aatgccgtga ccagcccata aaccaagcca ttctgccgcg cggtgttggc gacgaattgc
      961 tcgaaaccga ccttgacgat ttcgaacgcg gtctcggtct tcgtcaccaa tacgccatcg
     1021 gaaaacagct tgatctcgac gtcataggtc ccgatcggca cctcggccgg gagtggtatg
     1081 ccggtgcgaa acagcgtcgg cgtcaaaaat gtcaccgcgg aagtcgcctc gcgatagagg
     1141 ccatgttggg agcgcagccg cacaaaggcg ctgcggaacg cgtcgttcgg cacgacgtcg
     1201 gcataatcag gtccgacgcg ttgggtcagc agcacattgt tgagaccgag ctgttgccgc
     1261 cgctcgactt cgggcgaagc gatggcgtcg aacggccggt tggagaacag cgctagatag
     1321 gtcggcactt tcagaaattg ccggtagtcg gtattgatcc agatgccgaa tttgcgttcc
     1381 ttgcggcgtg tcaccatgtc ggcgcgcggc ccggagacgg tcaccaccag atcgtaactg
     1441 gtgcgggtgg ccggggtggt ggcatccttt tcgaccgagc cgaacagcac gagttcctcg
     1501 ccggaatagt tcggcgtcac ggtgacccgg tggttcgaga ccgacacgat cagccgctcg
     1561 gcgcgcgcag gaagcacggc gaaagccgcg cccagcacaa ccgccaggcc cagcgcagcg
     1621 agtgaggagc gagcggtcat ccggcgcctc cggtttcccg gatggtgaag agatcgtcgg
     1681 gacggatcac cagctcgatc gcaaagcgga tgccgactgc caaaatcagc agcccaagca
     1741 gcagccgcag atgttcgccg cgaatcttct gaccggcgcg ggcgccgaac tgcgcgccgg
     1801 tcacaccgcc gaccatcaga atcagcgcca gcaccgcgtc gacgagatgg ttggtgaccg
     1861 cgtgcagcat ggtggcgaac accatggtga ccagcgtcag caccatcgag gtgccgatca
     1921 cggtagaggt cggcacccgc agcagataaa tcatgatcgg gatcaaaatg aagccgccgc
     1981 cgatgcccat caccgcgccg atgaaaccga tgatgaggcc gaccacaacg accggtatca
     2041 ccgacaaata gatcttggag cgcttgaagc gcatcttgag cggcagtccg tgaatccagc
     2101 cgtggctgcc cgagcggcgc atcggcaccg cgacgccgcg ccgcgcccgc aggatcgcac
     2161 gcagcccctc ccagaacatc agtccgccga cggtggacag caggatgacg taggacagcg
     2221 cgatcagcag atcgagttgg cccagcgagc gcagcagcgt gaaggtccag acaccgagcg
     2281 cggtgccgat ggtgccgcca ctcaatagca ccagcgccag ggccggatcg atggcgcggc
     2341 ggcgccagta ggatatggcg cccgaaaacg aagacgcagc gatgtggctg gcgaccgagg
     2401 cgaccgcgac cgcgggcgcg atgccgacga agatcagcag cggcgtcatc agaaacccgc
     2461 cgccgatgcc gaacatgccg gacacgaaac cgaccgccgc gcccatcgcc aggatgagga
     2521 aaacattgac cgggatgtcg gcgatcggaa ggtaaagctg cacgcgcgtt tactttttgg
     2581 actttagcct tgagccggcc gaaaccgctc acggcaaggt ttcttgatcg agcaagcgac
     2641 gcaatgcgcg cgaacgcgga tcaactgccg cgcccctgca taaccgaaat cctccatggg
     2701 agggactaaa gattccgccg gaggcggctt ttttgacgcg gtctgccgca cactttcagg
     2761 cgtggtgaag aattgagatt aacaggcggc ggccgttaag gcctcccgat gggatcagtg
     2821 atgagcttca tcgctgaggc ttcctttggg ccgagccagc ggacctcctt ggtcgcagac
     2881 atggcttcca cgacagcaga ggagacgccc atcttcgtca tatagctcaa caggatcccc
     2941 gcaatgcgct gggtggaagc gacgggatcg ggcatcggag tcattgcctc aaactggtgg
     3001 acccccagca tggagccctc cacgccatag cgtatcttgc cgccggcata gacgagcacg
     3061 caggcgctga cacaataggc tgggcggacc tgacccgatg catcggcgac accgaccgct
     3121 gtcaccaggc cgcgcgatcg aatgatttcg cccatgatga cggcccgatt caaatcgccg
     3181 cccggagagg acagcaacac ggcatcgccg gcggcgagat gggcctcatc gagcctgtcc
     3241 cggaaccagt tcgcggtggc gacgccgatt tccccgctga tggcgagcgc gcgccggcca
     3301 tggcccgagc cgtcgaaatc ggcactggag atcagcgccg aagtcatggt cggggagaga
     3361 tatgactctt tccaataagc ccaggcctcc ggccgcgaca ggtcccgata ggcgcggatg
     3421 ccgacggcgg aaatcaagag aacgaagatg gcggccgacc agaaacggcg tttattacca
     3481 ttgccatgac gcggccgcac cgaaggctgg ccgataggtt tgcgcggtgg tgctactcgg
     3541 gggccagtcg gcgaccttcg gaaatggctt ccttcgtcat gaggatcgtc gacagacaat
     3601 tcatgctctc ttcgttcaaa agctgcgcgg gtcggcgttc ggcaagatcg tgcgcgccgc
     3661 ctgttagcgc gcttgtctac acggttttgt tggtggggta tatgacagcg acgccgccaa
     3721 cggcatcaat gcgcggcggc ggttcgcttg gtcaaaaccg gcttggccgc gggctttgcc
     3781 gcaccggcct gcgccggcgc tgagtcccat ccgcccggcg ggctcgccac gttgacggcg
     3841 tcgtcgggct gcggttctgg cgtgaaagtc tggatcgcga gctttgccgc cgcgagcgac
     3901 tgggggtcga gccgcttcgc gatatcttcg cgtttgcgca ccgcatccgc atcgccctgg
     3961 gccgcggcga ggctgaacca tttgaaggat tcggcgaggt tctgttcgac gccgatgccg
     4021 cgggcataga ggatgccgag gttgaattgg ctatccgcga cgccgcgctc ggcggccttg
     4081 cgaaaccatt gcgacgcggt cccgtagttg gcgcccttgc tgccgccgtc ggcgtcgagc
     4141 acggcgaggt tatgcatcgc cttggcgttt ccgcgttcgg cggcctgcat gtagtagcgc
     4201 cgcgcgatat cgacgtcttt tttcacactc agaccctttt cgtagagggt ccccagcctg
     4261 aagatcgccg gcaccacgcc ggcttgcgcc gaacggtcgt accatttcgc ggcctcgtcg
     4321 aaattggccg caacgccttt gccttcggcg tagcgcaggc cgatctcata ggccgccgat
     4381 ggatcgccct tgagggcggc cgcgcgcaat gcgggtccgc cgatggcgtc cggcaatctt
     4441 tccgtcggcg ggacctgtac cgtcgtgagt ttgcgaccgg tcggcccagc cagcgtggcc
     4501 ggaatcgtgc cggtgatatc gctgccggtg accggcgagg cgaagggtat cgccgtggcc
     4561 tgcgggattt caacagacgc ggtgctgtcg gaagcgttcg gcgcggaact attgaacgat
     4621 tgccggccga tcggcgtcgg cgaggtcagc gagggcatcg ccggcgcggg cgctgccggc
     4681 ttggcgccat cctccgcggg gggctgagcc gaaggcgcct gctcgctcga actctccatg
     4741 gcaggcagcg ccggaatact gctgccgtcg agcagcgtca tcgccatctt gaaagtggag
     4801 agcacgatca cgaccacgct cgcgccgacc agcagcgcgc gaatcttgga ggtgaccgcc
     4861 gatgtttcct tggtcttgtc gcgggcggca tccttgaacg ggctgcgggc ggccttgtcg
     4921 cccaccggtg cggccgcggc ggcctgggcg gcacggcgcg cggcggcaat gaaattcgat
     4981 gtgctggtct gctcgcgcgc gctcgccgtg atttcgctga tcgcgctttc cgaagcggca
     5041 atgcgttccg acggcgaagg tacccgccct gaaggccgtg tcccgggttc gagcgggtgg
     5101 tcgggcggca gttcgggcac gatcgcggct tgcggttgcg ccgcatgcga ctccagaatc
     5161 tc
//
LOCUS       DATNOI010000359        20628 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_448, whole
            genome shotgun sequence.
ACCESSION   DATNOI010000359 DATNOI010000000
VERSION     DATNOI010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 20628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20628
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..2248
                     /locus_tag="VN968_14375"
                     /pseudo
     CDS             1..2248
                     /locus_tag="VN968_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605036.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
     gene            2404..3099
                     /gene="pdeM"
                     /locus_tag="VN968_14380"
     CDS             2404..3099
                     /gene="pdeM"
                     /locus_tag="VN968_14380"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HXL30458.1"
                     /translation="MTRIAVGIASRSSVNIAGVTLVADLSGALFWEEQSLLVVSDLHL
                     EKGSSFAARGVLLPPYDTVATLGRLAGVIARHDPRMVIALGDSFHDRTAHDRLSAIDR
                     DALSALQARRDWIWISGNHDPALPCDLGGVVASEVAIGPIAFRHEPTGASGEIAGHLH
                     PKARVSTRGRSMERRCFACDGERAVMPAFGAYTGGLSIRDAAFAKIFQTPGLMAHVLG
                     DHRLHTIAASRCY"
     gene            complement(3306..4169)
                     /locus_tag="VN968_14385"
     CDS             complement(3306..4169)
                     /locus_tag="VN968_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXL30459.1"
                     /translation="MLDLELLRSFVSVVDSGGFTRAGERVHRTQSTVSQQIKRLEDDV
                     GQPLLNRNGKDVTPTEAGERLLSYARRLLSLAEEARDVLARPGNEGAIKLGIPEDFAA
                     YRLTKLLATFSRARPGLRLDVRADQSTFLRRDLERGDLDLALLKRDAGEKGGIAVWPE
                     RVHWVTSKSHPIDSTSGSIPLIGFPAGCLYRARAIHALESAGRAWHMAYTSSGLAGIQ
                     AAVAAGLGLSILSEMAIQADHRVLTAKDGFAPIDKTEVALVASPDASPATLRLADRLA
                     EFCDTVQAKAA"
     gene            4266..5180
                     /locus_tag="VN968_14390"
     CDS             4266..5180
                     /locus_tag="VN968_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXL30460.1"
                     /translation="MSFAPSIPVPRATFNPLPIYIGLFCVLWSFAFVAGKIGVTACPP
                     LILLTARFSLAGILILGISALRRDAWSLSWRDACVFAILGVANNALYLGLGYTGLQTV
                     SAGLAGLVVSANPVFTAGLAALFLGEALTWRKVAGLSLGIAGVSFIVWHRISVGTDSW
                     HGILFTLASLASIVAGTILFKLLAPKGSLWIGNGVQNLAAGVVLLPFAFTFADVGDVV
                     PSARFLGAFAFLVLGGSILAYLLWFHLLKVCGATAASAYHFLMPPLGMLFAWIILGEH
                     VEVRDLMGIVPVALGIYLVTRPVAASAQ"
     gene            5195..5980
                     /locus_tag="VN968_14395"
     CDS             5195..5980
                     /locus_tag="VN968_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL30461.1"
                     /translation="MNPLRITLIGGPTALIEIDGFRILTDPTFDAPGAYQLPHVKLEK
                     LSGPALKPEAIGKVDAVLLSHDQHADNLDHSGKDFLTKADRVLTTVAGAKRLGGRAEG
                     LAPWATTELTGNNGHSLTVTATPARHGPAGIEPLSGDVIGFVLTSKDNNIRPIYISGD
                     TVWYDGVAEVARRFKAAVVLPFAGAAQTRGPFHLTMDTNDTIETARAFPDAVIVPLHT
                     DGWAHFRQNANDLRASFDTLGFGPRLKILEPGVATTIEPPHHP"
     gene            complement(6039..7367)
                     /locus_tag="VN968_14400"
     CDS             complement(6039..7367)
                     /locus_tag="VN968_14400"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXL30462.1"
                     /translation="MASAPIPSGDYDSDVGAFSKFWLQGLSYLDGLPDRAKRSETEQA
                     DAAASLSQARQARCSFLGAHVGRVYRDLTDRLRSFRRIEELAVLAGEKIPGLCPTSLR
                     LTREASRVQAEKDGHEIDQGLFFNYVLGDEACGRHLCHAMLLPRKEAFEKLDPLKRHG
                     KVDLAHASVERKGKASIVSLKNSRYLNAEDDTTLKDVETAVDLAMLDPETTICVLRGS
                     VIEDGKYQGQRVFCTGINLTHIYYGRLPYLWYLVRDMGFVNKMYRGLAKPDVSPGEVH
                     GETIEKPWIAAVEKFAIGGGCQYLLAADYNIAAKDAYMTLPARKEGIIPGAANMRMPR
                     FVGDRITRQAIMSELRIECDSATGRMICDRIVEPEQMDQAIDEAVELLTNSGAVNASS
                     NRKAIRVVQEPLDVFRTYMAVYAREQAYCHFSPALVSNLERFWNAQRRKL"
     gene            7756..8121
                     /locus_tag="VN968_14405"
     CDS             7756..8121
                     /locus_tag="VN968_14405"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXL30463.1"
                     /translation="MAEYMAGNVDDYADGDRKVVVCGDKEVGVFRIGDEFYAWHNRCA
                     HRAGPVCQGRIMKRVLEPVASDKTTRSQAYDEAESHIVCPWHGYEFSIRSGFHQGNSR
                     VRLRKAELTIRDGEIHVSV"
     gene            8108..8467
                     /locus_tag="VN968_14410"
     CDS             8108..8467
                     /locus_tag="VN968_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30464.1"
                     /translation="MSASERDRRNDLYGWIDSAAPELLRASRELVEKGEASRVSDEAV
                     AQILTAAIRLYVAKSDGEERTFPPIAGTRDSEMTPTELLSAVSEMLRALHLGPMELAL
                     WYRRRPDEDTYFAKETS"
     gene            8464..9705
                     /locus_tag="VN968_14415"
     CDS             8464..9705
                     /locus_tag="VN968_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL30465.1"
                     /translation="MNIQGKFGPQYSPVQQIDVHTDTRDILAHARRNALKRGLQDWFV
                     CDIDAHHVETVSWKEIVTYIEDPVVRDNAMRFQAERLGAPPYGLNGDLGLRYQSVGGR
                     IPHQDGQREIVEETDVHRDVILTRRAMDSLGIDNMVVFPTPMLFLGMHPQPEMEVWLG
                     GAYNRWLIDRILSGDDRIKSLIYLPFNTPEEAERTVEELGDKKGVIGFCVTSTRNKPV
                     HHNDYMRLYSMIEERGKPLAFHAGYHWQDPSLATVNRFLGMHALGFVWCNMVHMTNWV
                     LNGIPERFPKLRTLWVESGLAWVPFLMQRLDDQYLMRPSEAPQLRRMPSEYMNQNCFY
                     TTQPMETTHPRALENTFEMIKAETQLMFASDWPHFDFDLPQEICDLPFLTEQAKRNIL
                     GLNAARIFNLDPTPVKKLTVI"
     gene            9918..10826
                     /locus_tag="VN968_14420"
     CDS             9918..10826
                     /locus_tag="VN968_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL30466.1"
                     /translation="MIELRQFRQFIAVAEELSFRRAADRLHMAQPPLTVTIKQIEEEL
                     GTLLIERTNRIARLTPAGRVFLEECKRTVMQAERAVAAARRAGAGLSGALRVTFVASA
                     AREILPAILRAFREQYPEVELILTEAQTAQQVTKIQTDEADVGFVIPPLRDADGLSVE
                     VLAKNQMVAALPENHPLAQLQSISLSDLASEDWIIFPARQGPGLHERVLTACAQAGFV
                     PRVGQQASQMDTIASLVAGGMGVGLVTRIIATANRPGVVFRDLTGIGTPVEYELAIAY
                     GRSTPLVDAFTAVIRSRANWVGPAAN"
     gene            10960..12324
                     /locus_tag="VN968_14425"
     CDS             10960..12324
                     /locus_tag="VN968_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HXL30467.1"
                     /translation="MTNRKKTNRKSNNKNHQKSSGQKPALKTGARQPFHVRAFLKALG
                     PGLITGASDDDPSGIGTYSQAGAQLGYGIGWTMLLTFPLMTAIQEISARVGRVTGHGI
                     AGNVCRHYSVLLLNVVVTLLFIANTINIAADLGAMADATKLLIGGPGIIYVVAFGVTS
                     VVAQIFLDYKRYVSVLKWLTLSLFAYVGALAFAHISWGEALAGVLVPRLTWNADYFTT
                     IVAIFGTTISPYLFFWQASQEAEDQRVDLTKHPLIERQYGAQKEFHRIRADTVVGMAF
                     SNLIALSIIVTAAATLHAAGKTDIQTSAQAAEALRPIAGTFAEVIFALGIVGTGLLAI
                     PVLAGATAYAVGEGRKWPVGLARKPKEAVAFYAVLALSAAIGIALNFTSINPISALYW
                     SAVVNGVLAVPVMVLLMIMARRKEVMGRFVIGGPPYWLGWLSTAAMLLSVVAMAIGIF
                     FGKS"
     gene            complement(12321..13112)
                     /locus_tag="VN968_14430"
     CDS             complement(12321..13112)
                     /locus_tag="VN968_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HXL30468.1"
                     /translation="MIARISPAVVGLGLALSAAFALSPAHAERLIVSVSNHRVTVTPN
                     YSGEELVLFGSVEKDATTPSTRTSYDLVVTVSGPRADMVTRRKERKFGIWINTDYRQF
                     LGVPTYLALFANRPFDAIASPEVQRRQQLGLNNVLLTQRVGPDYADVVPNDAFRSAFV
                     RLRSEHGLYREATSAVTFLTPTLFRTGIPLPAEVPIGTYDVEIKLFSDGALVTRAESA
                     FEIVKVGFEQFVANTARQNGLVYGLVTAFMAMMTGWMASIVFRKD"
     gene            complement(13109..14035)
                     /locus_tag="VN968_14435"
     CDS             complement(13109..14035)
                     /locus_tag="VN968_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXL30469.1"
                     /translation="MQLYLPIADIPVNVFLILAMGAAVGFVSGMFGIGGGFLMTPLLI
                     FIGIAPAVAVASVASHIAASSFSGAISYWRRRAIDPALALVLLTGGTVGTALGVWTFT
                     LLRSLGQLDLMIALSYVVLLTAVGGLMFWEGLRAILRTRRGMAVPMRRSGSHGWIHGL
                     PLKMRFKRSKIYLSVIPVVVVGLIIGFIGAVMGIGGGFILIPIMIYLLRVPTSTVIGT
                     SMVLTLVTMVFATMLHAVTNHLVDAVLALILMVGGVTGAQFGARAGQRIRGEHLRLLL
                     GLLILAVGIRFAIELVIRPDDLFTIRETGGAG"
     gene            complement(14264..17542)
                     /locus_tag="VN968_14440"
     CDS             complement(14264..17542)
                     /locus_tag="VN968_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30470.1"
                     /translation="MNSRLSWSVEGIDPSVRDRAEAAARRAGMSLSDWLNSTVGDFTP
                     PNLGSPTDQPPVPPIRETPDVLDIHQRLDSITRQIEQISRPAPHSEALRNNTVRGESA
                     VVARQLNEAISRLDARLSQISNPMPAKSAKLMDDQFDLVERAAAQVYRPSPPISPASL
                     DVAVAEIAARQNELDSAPRFLPPRSAPPMVPATPAGPDFSALERHLIQITSQIEALQH
                     PDGIEQSIAAFRGELAEIRHAITEAMPRQAIESIESEVRALSRRIDDTRQSGIDDSAL
                     AGIERALGEIREVLRSLTPAEQLAGYDDAIRNLGAKLDLILRASEDPSTVHKLESAIA
                     ALRAIVSNIASNDALARLSDDVHTLSSKVDQLARSEGPSDSFTILEQRIATLALTLES
                     REQPGPSESSEQLEGAVRALSDRLDRMPVGNDNTSAFAHLEQRVSYLLERLEASTDHR
                     AGHLGRVEDGLQDILRQLENQQARFAAVAESSRAPAEPADSGVIDIVKRELSDIRFSQ
                     TEAGRHTQESLEAVHSTLGHVVDRLAIIEGDLRTVRVQPVAPQAETPPAPPSAPAAAP
                     AIAIAPQPKPELPNPAASQSHFATAPREFQAAPIQLPALAAAAPRAISEILDQHAAPP
                     RATIEPGLPPDHPLEPGTRPSGRVPSPSERIAASESAISEITAGTREPTSTSNFIAAA
                     RRAAQAAAAAPPVDKPARGPLKDPARDKAKVTSTEASTVTSKIRSVLVGASVVIIVLG
                     TFKLAMTLLDGGSVPALPATESSGEQTPLIQWPAEDAAKPPAPAMPSMTSPTPIGRQS
                     FNGSTPNASDDTASAEILPNAPIPSASPLSASDITGSVPIAQVAPVSHKLTTVQVPPT
                     ERLPDAIGGPVLRAAALKGDPAAAYEIGLRFADGKGVAPNLDEAAKWYDRAAQAGVVP
                     AVFRLGTFYEKGLSVKKDIDIARRYYMQAAERGNAKAMHNLAVLDADGGGKGANYKDA
                     SHWFRKAADRGVADSQFNLGILYARGIGVEQNLAESFKWFSLAAAQGDADSIRKRDDI
                     AKRLDAQSLAAAKLAIQTFTPEPQPDDAINVAGPAGGWDSSPAQAGAAKPAAKPVLTK
                     RADR"
     gene            17898..19688
                     /locus_tag="VN968_14445"
     CDS             17898..19688
                     /locus_tag="VN968_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HXL30471.1"
                     /translation="MPSYKAPLEDVSFLLNDVFQIDRYDNLPGFSDASADVREAVLNE
                     AAKLSEQVLQPLNRVGDLEGCVRHDDGSVTTPKGFKEAYRQVAEGGWLGLSAPTEYGG
                     QGLPVTLSQAVSEFQISANMAFSMYGGLTMGATAALMVHGKPEQKAMFVPKMVAGEWT
                     GTMNLTEPQCGTDLGLLRTKAVKQADGSYKISGTKIFISAGEHDLTENIIHLVLARIE
                     GAPSGIKGVSLFVVPKTLVNADGSLGARNGVTCGSIEHKMGIHGNSTCVMNYDGATGW
                     LIGEENKGMQGMFVMMNEARLGVAVQGLAQSEVAYQNAVAYARDRLQGRSLSGAKAPD
                     KPADPIIVHPDVRRTLLTIRAFNEAARAMVVWTALKSDVAHRSSDPRDRQEADDHMGL
                     MTPVMKGVLTDVGFSNAVLAQQMFGGHGYIAETGMEQFVRDARIAMIYEGANGIQALD
                     LVGRKLPRDGGRAVMAFFGEVAAFAKDHGADEAMKPYVAPLSTALGHLQQATTWLMQN
                     AMMKPDNAGAGATDYMQLFGLVTFAYMWARMAKVALDKIAASGATPYLTTKLVTGRFF
                     MERMLPETALHLARIQTGSATTMELAAEAF"
     gene            19778..>20628
                     /locus_tag="VN968_14450"
     CDS             19778..>20628
                     /locus_tag="VN968_14450"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195228.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXL30472.1"
                     /translation="MPEAYIYDHVRTPRGRGKADGALHEVTALALATVPLKALKERNN
                     LAEDVVDDVILGVVDPVGEAGSDIARFAALKAGLGEAVPGVQISRFCASGLDAVNFAA
                     AQIMSGQHELTIGGGAESMSRVGIGASGGAWPMDPSIAVPAYFMPQGVSADLIATKYG
                     FSRDDVDAYAVRSQQRAAKAWDEGRFKNSVVPVKDVNGLTILAKDEHMRPTTTMQSLA
                     SLQPSFVTVGQMGGFDAVAIQSHPEVERINYVHHAGNSSGSVDGAGAVLLGSKEAGAK
                     YGLKARA"
BASE COUNT         3734 a         6385 c         6643 g         3866 t
ORIGIN      
        1 ggtcgatatc gcgcgcaacc tggaagcgcc gatcgccgaa atgaatctgc cgatcaaggt
       61 cgagacccgc accggcgata cgccggtgtc gcggcggcag cggcagcggc gctatccgcc
      121 ggatattctt ctgaccacgc ccgaacagct ggcgctgttg ctgtcgtccg acgacgcgcc
      181 gtttctgttc gcctagctca ggcgcatcgt gctcgacgag ttgcatgcgc tggtcacttc
      241 caagcgtggc gatctgttgt cgttgggtct cgcgcggctg tggcaactgg cgccgcagat
      301 gcgctcgatc gggctgtcgg ccaccgtcgc ggcgccggaa tcgctggcgc gatttctggt
      361 gccgcagcgc gatggcgcaa gcgaagccgc cgacatcgtc gtcgccggcg gagccgcggc
      421 gcctgttgtc gaaatgctcg acaccaggga acgcctgccc tgggccggcc atagcgcgcg
      481 ccacgcgctc aatgaggtct acgacctgat caagcagaac aggaccacgc tggtgttcgt
      541 caacacccgc agccaggccg agatgctgtt tcaggatctg tggcgcatga acgacgatgg
      601 ccttgccatt gccctgcatc acggctcgct cgacgtcgcg cagcgccgca aggtcgagga
      661 cgcaatggcg aagggcaaac tgcgcggcgt ggtctgtacc tcctcgctcg atctcggcgt
      721 cgactggggc gacgtcgatc tcgtgatcaa tatcggcgcg ccgaaaggcg cctcgcgcct
      781 gatgcagagg ataggccgcg ccaaccaccg catcgatgaa gcctcgcgcg cggtgctggt
      841 gccggccaat cgcttcgagg tgctggaatg cgccgtggcg atcgacgcga ttgccgaaaa
      901 cgcccaagac accccgccgc tgcgcaccgg cgcgctcgat gtgctggcgc agcacgtgct
      961 cggctgcgcc tgcggcgagc cgtttctctc cgaccagctc tatcgcgaag tgctgacggc
     1021 agcaccctat tccagcctga cgcggaccga tttcgacgat gtggtcgatt tcgtcgccac
     1081 cggcggctac gcgctgaaaa cctacgaacg ttttgcgcgc atcaagcagg acaaaagcgg
     1141 gaaatggcgc gtcgccaatc cactgacgcg acaaagctat cgtttgaatg tcggtaccat
     1201 cgtcgaggag accatgctga aagtgcggct ggtgcgctcg cgcggaggcg gttcaggatc
     1261 gaccggcgcg ctcgggcgcg gcggccggat gttggggcat atcgaggaag tcttcatcga
     1321 aggcctcgtg atcggcgaca cctttgtctt tggcggcgag gtcgtgcgtt acgaagcgct
     1381 cgtagaggat caggtttatg tctcccgggc ccacgacaag gatgcaaaag tgccgtccta
     1441 tatgggcggc aagtttccgc tctcgaccta tctggccgag cgcgtccgca aattgctcga
     1501 cgacaagcgc gcctggaatg cgctgccgga tcaggtgcgc gactggctgt cgctgcagcg
     1561 ggatatttcg cgggtgcccg gcgtgcgcga actgctggtc gaaacctttc cacgcggcaa
     1621 caagcactac ctcgtctgct atccctttga aggtcggctg gcgcatcaga cgctcggcat
     1681 gctgctgacg cggcggatgg agcgcgcgcg ggtgcggccg cttggctttg tcgccaatga
     1741 atatgcgctt gccgtctggg gactcggcga catgtcgttc atgatccggc agggccggct
     1801 cgatctcgac gcgctgttcg atccggacat gctgggcgac gatctcgaag cctggctggc
     1861 ggaatccgcg ctgatgaaac gcacttttcg aagctgcgcg atcatctccg gattgatcgc
     1921 gcggcggttc accggcgagg aaaaaacccg gcggcaggtg ctgttttcga ccgacctgat
     1981 ctacgacgtg ctgcgaaaac accagccgga ccacgtgctg ttgcgcgccg cgcgtgccga
     2041 tgccgcgacc gggttgctcg atcttcgccg gctaggtgat atgctgctgc gaatcaaggg
     2101 gcggatcatc cacaaggaac tcgacaaggt ttcgccgttg gcagtgccgg tgatgctgga
     2161 aatcggccgt gaatcagttt atggcgaagc ctccgacgag ttgctggcgg aagccgccga
     2221 tgaactcgtc aaagaggcga tgtcgtagaa taaaatgaac ttgttgtact ccgacccatc
     2281 aacatcgtca tgcccgggcc cgtcccgggc atccgcgtct tttttcttca tggagccaag
     2341 acgtggatgg ccgggcacag gcgagcggaa gcgacgccgt ccttcagacg gctatgcccg
     2401 gccatgacga gaattgcagt tggcattgcg agtcgttcct cagtgaacat cgcgggcgtg
     2461 accctggtcg ccgatctctc cggcgcgttg ttctgggaag aacaaagcct gctcgtcgtc
     2521 tcggatctgc atctggaaaa aggctccagt tttgccgcgc gcggcgtgct gctgccgccc
     2581 tacgataccg tggcaacact cgggcgcctc gccggcgtga tcgcgcggca cgacccgcgc
     2641 atggtgatcg cgctcggcga cagttttcat gaccgcaccg cccatgaccg gctgtccgca
     2701 atagatcgcg acgcgctctc ggcgttgcag gcgcggcgcg actggatctg gatttccggc
     2761 aatcacgatc ccgcgctccc ctgcgatctc ggcggcgtgg tcgccagcga agtcgccatc
     2821 gggccgatcg cgtttcgtca cgagccgacc ggcgcatcag gtgaaatcgc cggacatctg
     2881 catcccaagg cgcgggtgtc gacacgcgga cgttcgatgg aacggcgctg ttttgcctgc
     2941 gatggcgagc gcgcggtgat gcccgcgttc ggcgcctaca ccgggggcct cagcattcgc
     3001 gacgcggcat ttgcaaaaat attccagacc ccgggcttga tggcgcatgt gctcggcgat
     3061 caccgcctgc acaccatcgc cgcctcgcgc tgttactgag catttcaaga tttatggtgt
     3121 cgagttcagc cacacctctc ccgttgggag aggtcggatt gcaaagcaat ccgggtgagg
     3181 ggttacggcc tatcgagaga tcgtaacccc tcacccggcc gcgatgctga gcatcgcggc
     3241 caccctctcc cacaaggggg cccacaaggg gagaggggtg caccttccgc gctgcaagac
     3301 taacgctacg ccgccttcgc ctgcaccgtg tcgcagaact ccgcaagacg atcggccagc
     3361 cgcagcgtgg cgggactggc gtccggcgaa gccaccagcg ccacttccgt cttgtcgatc
     3421 ggcgcaaaac catcctttgc ggtgagcacg cgatggtcgg cctggatcgc catctcggaa
     3481 agaatactca ggcccaatcc ggcggcgacg gcggcctgga tgccggcgag gcctgaactg
     3541 gtataggcca tgtgccaagc ccgcccggcg ctttcgagcg catggatcgc gcgcgcccga
     3601 tagaggcagc cggcgggaaa gccgatcagc ggtatggatc cgctcgttga gtcgatcgga
     3661 tggctcttgc tggtgaccca atgcacgcgc tccggccaca ccgcgatgcc gcccttttcg
     3721 ccggcgtcgc gcttgagcag cgctagatcg aggtcgccgc gttcgagatc gcgccgcaga
     3781 aaagtgctct ggtccgcccg cacatcgagc cgcaagcccg gtcgcgcgcg ggaaaacgtt
     3841 gccagcagct ttgtcaggcg ataggccgcg aaatcctccg ggattccgag cttgatcgcg
     3901 ccctcattgc ccggccgcgc gagcacgtcg cgggcttctt cggccagcga caacagccgt
     3961 cgtgcatagg acaacagccg ttcgccggct tcggtcggcg tcacgtcctt tccgttgcgg
     4021 ttcaaaagcg gctggccgac atcgtcctcc agccgcttga tctgctggct gacggtcgat
     4081 tgcgtgcgat gaacgcgttc cccggcgcgc gtaaagccgc cggaatccac caccgaaaca
     4141 aagctgcgca gcagttcgag atcgagcatg ggtctgccca ttcacatttc cactggaata
     4201 cagtttatca tttaatttcc aaatatcaag gtgcgagcct acatcgaatt caaaggagaa
     4261 ttcccatgtc gtttgcgccg tccattcccg ttccccgcgc caccttcaac ccgctgccga
     4321 tctatatcgg cttgttctgc gtgctctgga gttttgcttt tgtcgcagga aaaatcgggg
     4381 tcaccgcttg cccgccgctg atcctgctca ccgcgcgatt ttcgctggcg ggaattctga
     4441 tcctcggaat ctccgcgctg cgccgcgatg cctggtcgct gtcgtggcgc gacgcctgcg
     4501 tgttcgcaat tctcggtgtc gccaacaacg cgctttatct cggcctcggc tataccggac
     4561 tgcagaccgt gtcggccggc ctcgccggcc tggtggtcag cgccaatccg gtgtttaccg
     4621 cagggctggc cgcgcttttt ctcggggagg cgctgacctg gcgcaaggtg gcggggctat
     4681 cgcttggcat tgccggcgtc agtttcattg tctggcatcg catctcggtc ggcaccgaca
     4741 gctggcacgg catcctgttc acactggcat cattggcctc gatcgtcgcg ggcaccatcc
     4801 tgttcaagct gctcgcgcca aagggcagcc tctggatcgg caatggtgtg caaaatctcg
     4861 ccgcgggtgt cgtgctgctc ccgttcgcgt tcacttttgc cgatgtcggc gatgtcgtac
     4921 caagcgcacg gtttttggga gcattcgcat ttctggtgct gggcgggtcg atccttgcct
     4981 atctgttgtg gtttcatctt ttgaaggtgt gcggagccac agccgccagc gcctatcact
     5041 ttttgatgcc gccgcttggc atgctgttcg cctggattat tcttggcgag cacgtcgaag
     5101 tcagggattt gatggggatc gttccagtgg cgctcggtat ctatctggtg acgcgtcctg
     5161 tcgcggcgtc ggcgcaatag agagggaggt ccgcatgaat ccgcttcgta tcacgctgat
     5221 cggcggcccc accgcgctga tcgagatcga cggctttcgg atcctgaccg atccgacctt
     5281 cgatgcgccc ggcgcctatc aattgccgca tgtgaagctt gagaagttgt ccggcccggc
     5341 gctcaagccc gaggcgatcg gcaaggtcga tgccgtgctg ctcagtcacg accagcacgc
     5401 ggacaatctc gatcattccg gcaaggattt tctgaccaaa gccgatcgcg tgctgacgac
     5461 ggttgcgggc gcgaagcggc tcggcggccg cgccgaaggt cttgcgccct gggcgaccac
     5521 cgaactgacc ggcaacaatg gccattccct gaccgtcacc gccacgcccg cgcgtcatgg
     5581 tccggccgga atcgaaccgc tgtccggcga cgtcatcggc ttcgtgctga cctcgaaaga
     5641 caacaacatc cgtccgatct acatcagcgg cgataccgtt tggtatgacg gcgtcgccga
     5701 agtcgcgcgg cgcttcaagg ccgccgtggt gctgccattc gcgggcgcgg cgcagacgcg
     5761 cggaccgttt catctcacca tggataccaa cgacaccatc gaaaccgcgc gggcgtttcc
     5821 ggatgccgtg atcgtcccgc tgcacaccga tggatgggcg cacttccgcc agaacgcgaa
     5881 cgatctgcgc gcgtcgttcg atacgctcgg cttcgggccg cgcctgaaaa tcctcgagcc
     5941 aggtgttgca accaccatcg agccgccgca tcacccttaa atggcgttgc caactgccgg
     6001 agcgccgccg cacccctgaa gccgcttact tacgccgctc agagctttcg ccgctgggca
     6061 ttccagaagc gctcgagatt gctgaccagc gccggcgaaa agtggcagta agcctgttct
     6121 cgggcgtata ccgccatata ggtcctgaac acatcaagcg gttcctgaac aacccggatc
     6181 gccttgcggt tacttgatgc gtttacagcg cccgaatttg taagcaattc gacggcctcg
     6241 tcgattgcct ggtccatctg ttccggctcg acgatacgat cgcagatcat ccgtccggtc
     6301 gcactgtcgc actctatccg gagttcgctc atgatggctt gacgggtgat gcgatctccg
     6361 acgaagcggg gcatccgcat gttggcggcg ccagggatga tcccttcctt gcgtgctgga
     6421 agcgtcatgt aggcatcttt cgccgctatg ttgtagtcgg cggcaagcag gtactgacat
     6481 cctcctccga ttgcgaactt ctcaaccgct gcgatccagg gcttttcaat cgtttcgcca
     6541 tggacttcgc cgggcgaaac atcgggcttt gccaggcccc gatacatctt gttgacgaag
     6601 cccatgtcgc gcacgagata ccaaagatag ggcaggcggc catagtagat gtgcgtgagg
     6661 ttgatcccgg tacaaaagac ccgttgtccc tggtatttcc catcctcgat gacactgcct
     6721 cgaagtacgc agatcgtcgt ctcgggatcg agcatggcga gatcgacggc tgtctcgaca
     6781 tcctttaacg tcgtgtcgtc ctcggcattg agatatcgcg aatttttcag cgacacgatc
     6841 gaggctttgc cttttcgttc gacgctggca tgggcgagat cgactttccc gtgtcgcttc
     6901 aacggatcca gcttctcgaa tgcctccttt cgcggaagca gcatcgcgtg gcataggtga
     6961 cgaccgcatg cttcatcgcc aagcacgtag ttgaaaaaca atccctgatc gatctcgtga
     7021 ccgtccttct cggcttgaac gcgactggct tccctggtga ggcgcaggga cgtcgggcaa
     7081 agacctggga tcttctcgcc cgccagcacc gccaattctt caatcctgcg gaatgaacgc
     7141 aacctgtcag taaggtcccg atagacccgc cccacatggg cgccaagaaa actgcaacgc
     7201 gcctgccgcg cttgcgaaag tgacgccgcg gcgtctgcct gctcggtttc gcttcgtttg
     7261 gcccgatccg gaagtccatc aaggtacgaa agaccttgaa gccagaattt cgagaaggca
     7321 ccgacatcgc tgtcgtagtc gccggatgga atcggcgcgc tcgccatccg gcccacagct
     7381 ctcgaatcgg ttttctcgct tgcctgggag gcgggcattg aacgacctct ggatgtctgg
     7441 tgccagcttg tggcgctgca ctcagctcat tcatggtaat tgatttcaaa agggtctgtc
     7501 ctcatgcgaa cgaatgacgg ttcggcttcg gtcgttgttg cgctgtcgca acatgcaatg
     7561 cgtcggtgat ggcgtatcgt gaggcatggc ttgctcgcga tacacgccag ctatcgctcc
     7621 atccaaattt agtattggcg gctcgaccaa gcgccgccga tgatggcacg ccaaataccg
     7681 atgctgatcg gcggtagctg gaccggcgac cggtagctcc gctcgcttgc ggcaaaaaga
     7741 aattggagga ggcgcatggc cgagtacatg gctggcaacg tcgacgatta cgccgatggc
     7801 gatcgcaagg tcgtcgtgtg cggggacaag gaagtcggcg tctttcggat cggggatgaa
     7861 ttctacgcct ggcataaccg atgcgcgcac cgcgccggcc cggtctgcca gggccgcatc
     7921 atgaagcgcg tgctcgagcc ggtcgcgagc gacaagacta cgcggtcgca agcctatgac
     7981 gaagccgaga gccacatcgt ctgcccgtgg cacggctacg aattcagcat cagaagcgga
     8041 tttcatcagg gcaacagccg ggtgcggctg cgcaaggccg aactgacgat ccgggacgga
     8101 gagatccatg tcagcgtctg aacgcgaccg cagaaacgat ctttatggct ggatcgattc
     8161 cgccgcgccg gaattgctgc gtgcctcgcg cgaactggtc gagaagggcg aggcgtcgcg
     8221 cgtttcggat gaagccgtcg cgcaaatcct gacagcggcg atccgcctct atgtcgcgaa
     8281 atcggacggc gaagaacgga cctttccgcc gatcgcgggg acccgcgaca gcgagatgac
     8341 gcccaccgaa ttgctatcgg cggtttccga aatgctgcgc gcgctgcatc tcggcccgat
     8401 ggaactggcg ctctggtatc ggcggcgccc ggacgaggac acctatttcg cgaaagaaac
     8461 atcatgaata ttcaaggcaa gttcggaccg caatattcgc cggtacaaca gatcgacgtt
     8521 cataccgaca cccgcgacat cctcgcccat gccaggcgca acgcgctcaa gcgcggtctg
     8581 caggactggt tcgtttgcga catcgacgcc caccacgtcg agacggtgtc ctggaaggag
     8641 atcgtcacct acatcgagga tcccgtggtc cgcgataacg cgatgcgatt tcaggcggag
     8701 cggctcggcg caccgcccta tgggttgaac ggcgacctcg ggctgcgcta ccagtcggtc
     8761 ggtggacgta tcccgcatca ggatggtcag cgggaaatcg tcgaggaaac cgatgtccat
     8821 cgcgacgtca tcttgacgcg ccgggcgatg gattcgctcg gcatcgacaa catggtggtg
     8881 tttccgacgc cgatgctgtt tctcggcatg cacccccagc cggaaatgga agtctggctc
     8941 ggcggcgcct ataatcgctg gctgatcgat cgcattctgt ccggggacga ccggataaaa
     9001 tccctgatct atctgccgtt caatacgccc gaagaggccg agcggaccgt cgaggagctg
     9061 ggcgacaaaa agggcgtgat cggcttctgt gtaacgagca cccgcaacaa gccggtgcac
     9121 cacaacgact atatgcgcct ctattccatg atcgaggagc gcggcaagcc gctggcgttt
     9181 cacgccggct atcactggca ggatccttcg ctggcgaccg tgaaccggtt tctcggcatg
     9241 cacgcgctgg gctttgtctg gtgcaacatg gtccatatga cgaactgggt gctgaacggg
     9301 attcccgagc gcttcccgaa actcaggacg ctgtgggtcg aaagcggatt ggcgtgggtg
     9361 cccttcctga tgcagcggct cgacgaccag tatctgatgc gcccctccga ggctccgcag
     9421 ctcagacgca tgcccagcga gtacatgaac cagaattgtt tttatacgac ccagccgatg
     9481 gagacgacgc atccgagggc gctggaaaat accttcgaga tgatcaaggc cgaaacccag
     9541 ctgatgttcg catcggactg gccgcatttc gatttcgacc tgccgcagga aatctgcgat
     9601 ctgccgtttt tgaccgaaca ggccaagcgc aacattctcg ggctgaacgc ggcgcgcatc
     9661 ttcaacctcg atcccacgcc ggtgaagaaa ttgacggtta tctgagcaag gcaagcccgg
     9721 ccgcctgaac cgagccggcc aaaaagtgcc cccccaccct aaccccgcgt gcggcggagg
     9781 gcgggggtgg gggctttcat tttcctcgag cgatccggac cggcgcgtcg cctgctcgcg
     9841 aacctgaatt cgttcgtgtt ttttgccttg atccgatacc ctgttcacaa cggaaatggg
     9901 ggacgtcggg ccagggcatg atcgagttgc gtcagtttcg acagtttatc gccgttgcag
     9961 aagaactcag ttttcgccgc gccgccgatc gtttgcacat ggcgcagccg ccgctcacgg
    10021 tcacgatcaa acagatcgag gaagaactgg gaacactgct gatcgagcgc accaatcgta
    10081 tcgcgcgcct gacgccggcg ggccgggtat ttctcgagga atgcaaacgc acagtgatgc
    10141 aggcggagcg cgcggtagcc gcggcacgcc gcgccggagc gggcctgagc ggcgcgctgc
    10201 gggtgacatt tgtggcgagc gcagcccggg aaatcctgcc ggccatcctg cgcgcgtttc
    10261 gcgaacaata tccagaggtc gaattgatac tcacggaagc gcagaccgcg cagcaggtca
    10321 ccaaaatcca gaccgatgaa gccgacgttg gcttcgtgat tcctccgctt cgcgatgccg
    10381 acggactgag tgtcgaggtg ctggcgaaaa accagatggt cgcggcactt cccgaaaacc
    10441 atccgctcgc ccaacttcaa tcgatctcgc tgtcggatct ggcgtccgaa gactggatca
    10501 tttttccggc gcggcagggg ccgggcctgc acgaacgcgt gcttaccgcc tgcgcgcagg
    10561 ccggtttcgt gccgcgggtc ggccagcaag cctcgcagat ggacaccatc gccagtcttg
    10621 tggccggcgg catgggggtc gggctcgtta cccgcatcat cgcaaccgcc aaccggcccg
    10681 gcgtcgtatt tcgcgatctc accggaatag gcacgccggt cgaatacgaa ctggcgatcg
    10741 cctatggccg ctcgacgccg ctggtggatg cgttcacagc cgtgatccgg tcccgcgcga
    10801 actgggtggg accggccgcc aactagcctt cgcgccaaag ccgcgtgcgc cgtcgtgcct
    10861 ccccgcctgg tttgcttgcc gcgccgggcg ccctcaggca agttcgcggc ccggaaccga
    10921 aacctgtcgg gatcgttgtc gcggcggctg gacttgggca tgacaaatcg caaaaagacc
    10981 aatcgcaaga gcaacaacaa gaatcatcag aagtccagcg gccaaaagcc ggcactgaag
    11041 accggggccc gccagccgtt tcacgtccgc gcgtttctca aagcgctcgg accgggattg
    11101 attaccggcg cctccgacga cgatccgtcc ggcatcggca cctatagcca ggccggcgcg
    11161 cagctcggct acggcatcgg ctggaccatg ctgctgacgt ttccgttgat gacggcgatc
    11221 caggaaatct cggcaagggt gggccgggtc accggccacg gcattgccgg caatgtgtgc
    11281 cggcattatt cggtgctgct gctcaacgtg gtggtaacgc tgctgttcat cgccaacacc
    11341 atcaacatcg cggctgatct gggcgcgatg gcggatgcca caaaactcct gatcggcggg
    11401 cccggcatta tttacgtggt ggcgttcggg gttacctcgg tggtcgcgca gatctttctc
    11461 gactataagc gctacgtgtc ggtgctgaaa tggctgacgc tcagcctgtt cgcctatgtc
    11521 ggggcgctcg cctttgcgca catttcctgg ggcgaggcgc tggctggtgt tcttgtgccg
    11581 cggctcacct ggaatgcgga ctatttcacc accatcgtcg ccattttcgg cactacgatc
    11641 tcgccctatc tgtttttctg gcaggcgtcg caggaagccg aggatcagcg tgtcgatctc
    11701 accaagcatc ccctgatcga gcggcaatat ggcgcgcaaa aggaatttca tcgcattcgc
    11761 gccgacacag tcgtcggcat ggcattctcg aacctgatcg cgctgtcgat catcgtcacc
    11821 gcggcggcga cgctgcatgc cgccggcaaa accgatatcc agacctcggc gcaagcggcc
    11881 gaagcgctgc gcccgatcgc cggaacattc gcggaagtga tttttgcgct gggcatcgtc
    11941 ggcaccggac tgttggccat tccggtgctc gcgggggcca ccgcctacgc cgtgggagag
    12001 gggcggaaat ggccggtcgg gctcgcgcgc aagccgaagg aggcggttgc gttctatgcg
    12061 gtgctggcgc tgtcggcagc aatcgggatc gcgctcaact tcacctcgat caatccgatc
    12121 tcggcgctgt actggagtgc ggtggtcaac ggcgtgctcg ccgtcccggt gatggtgctg
    12181 ctgatgatca tggcgcgtcg caaggaggtg atggggcgtt ttgtgatcgg cgggccgcca
    12241 tattggctgg gctggctctc gaccgcggcg atgctgctca gcgtggtcgc aatggcgatc
    12301 gggatttttt tcggaaaatc ctaatccttg cgaaacacga tcgaggccat ccagccggtc
    12361 atcatcgcca tgaaggccgt gaccaatcca tagaccaagc cgttctgccg cgcggtgttg
    12421 gcgacgaact gttcgaagcc gaccttgacg atttcgaacg ccgattcggc ccgcgtcacc
    12481 aaggcgccat cggaaaacag tttgatttcg acgtcgtagg tgccgatcgg cacttcggcg
    12541 ggcagcggaa tgccggtgcg aaacaatgtc ggcgtcaaga atgtcacggc cgaagtcgcc
    12601 tcgcgataga ggccatgttc ggagcgcagc cgcacaaagg cgctgcggaa cgcgtcgttc
    12661 ggcaccacgt cggcataatc aggtccgacg cgctgggtga gcagcacatt gttgaggccg
    12721 agctgttggc gccgctgcac ttccggcgaa gcgatggcgt cgaacggccg attggcgaac
    12781 aacgccagat aggtcggaac gccgagaaac tgccggtagt cggtgttgat ccagatgccg
    12841 aatttacgtt ccttgcgccg tgtcaccatg tccgcgcgcg gtccggagac ggtcaccacc
    12901 agatcgtaac tggtgcgggt cgaaggcgtg gttgcatcct tttcgaccga gccgaacagc
    12961 accagctcct cgccggaata gttcggcgtc accgtcaccc ggtggttcga gaccgagacg
    13021 atcagccgct cggcatgcgc gggcgatagc gcgaaagccg cgctcagcgc cagcccaagg
    13081 ccgaccaccg caggtgagat acgcgcgatc atccggcgcc tccggtttcc cggatggtga
    13141 agagatcgtc gggacggatc accagttcga tcgcaaaccg gatgccgacc gccagaatca
    13201 gcagtccgag cagcagccgc agatgttcgc cgcgaatcct ctgaccggcg cgggcgccga
    13261 actgcgcacc ggtcacgccg ccgaccatca ggatcagcgc cagcacggca tcgacgagat
    13321 ggttggtgac cgcgtgcagc atggtggcaa acaccatggt gaccagcgtc agcaccatcg
    13381 aggtgccgat caccgtcgag gtcggcaccc gcagcagata gatcatgatc gggatcagga
    13441 tgaagccgcc gccaatgccc atcaccgcgc cgataaagcc gatgatcagg ccgaccacca
    13501 cgaccgggat caccgacaga tagatcttgg agcgcttgaa gcgcatcttc agcggcaagc
    13561 catggatcca gccatggctg ccggagcggc gcatcggtac cgccatgccg cgccgcgtcc
    13621 gcaggatcgc gcgcaggcct tcccagaaca tcaagccgcc gacggcggtc agcaaaacga
    13681 cgtaggacag cgcaatcatg aggtcgagct ggccgagcga gcgcagcagc gtgaaggtcc
    13741 agacgccgag cgcggtgccg acagtgccgc cggtcaacag caccagcgcc agcgccggat
    13801 cgatggcacg gcggcgccag taggagatgg cgccggagaa cgacgaggcc gcgatatggc
    13861 tggcgacgga ggcgactgcg accgcgggcg caatgccgat aaaaatcaac agcggtgtca
    13921 tcagaaatcc gccaccgatg ccgaacatgc cggacacgaa accgaccgcc gcgcccatcg
    13981 ccaggatgag gaaaacattg accggaatgt cggcgattgg aaggtaaagc tgcacgcgcg
    14041 tttgcttctt ggattttagc cttgaagctg gccgaaaccg ctcacggcaa gatttctgat
    14101 cgagcaagtg ggattcgtcc gcacgggatg ggcgcgaacg agggtcaaat gccgcgcccc
    14161 tgcatatccg aaatcgacca tgggagggac taaagaatcc gcttgcaacg gcttttttga
    14221 cgcagccggc cgcgcgcctt caagtgtggc gaatgatttc agattagcgg tcggcgcgct
    14281 tggtcaaaac cggcttggcc gcgggctttg ccgcgcctgc ctgtgccggc gatgagtccc
    14341 aaccgccggc cgggcccgcc acgttgatgg cgtcgtcggg ctgcggttct ggcgtgaagg
    14401 tctggatcgc gagcttggcc gcagccagcg actgggcgtc gagccgcttg gcgatatcgt
    14461 cacgttttcg gatcgagtcc gcgtcgccct gcgccgccgc caggctgaac catttgaacg
    14521 attcggcgag gttctgttca acgccgatgc cgcgggcata gagaatgccg aggttgaact
    14581 ggctgtccgc gacgccgcga tcggccgcct tgcggaacca atgggacgcg tccttgtagt
    14641 tggcgccctt gccgccgccg tcggcatcga gcacggccag attatgcatc gccttggcat
    14701 ttccgcgctc ggccgcctgc atgtagtagc ggcgcgcgat atcaatgtcc tttttcacgc
    14761 tgagaccctt ttcgtagaag gtccccagcc tgaagaccgc cggcaccacg ccggcctgcg
    14821 ctgcgcggtc ataccatttc gcggcctcat ccaaattcgg cgcaacgcct ttgccgtcgg
    14881 caaagcgcag gccgatctca taggcagctg cgggatcgcc cttcaacgcg gccgcgcgca
    14941 ataccgggcc gccgatggcg tccggcaatc tttccgtcgg cgggacctgc accgtcgtca
    15001 gtttgtgact gactggcgct acctgcgcga tcggaaccga gccggtgatg tcgctggcgg
    15061 agagcggcga agcggatggt atcggcgcgt tcggcaggat ttcggccgac gcggtgtcgt
    15121 cggaggcgtt cggagtggaa ccattgaacg attgccggcc gatcggcgtc ggcgacgtca
    15181 tcgaaggcat ggccggcgct ggcggcttgg cggcgtcctc cgcgggccat tgaattaaag
    15241 gcgtctgctc gcccgaactc tctgtcgccg gcagcgccgg aacgctgccg ccgtcaagca
    15301 gcgtcatcgc cagcttgaaa gtgccgagca cgatgatcac cacgctggcg ccgaccagca
    15361 ccgagcgaat cttggaggtg actgtcgatg cttccgtcga tgtcaccttg gccttgtcgc
    15421 gggcgggatc cttgagcggg ccgcgggcgg gcttgtcgac gggtggcgcg gccgcggcgg
    15481 cctgggcggc acggcgcgcg gcggcaatga aattcgaagt gctggttggc tcgcgcgtgc
    15541 ccgcggtaat ttcgctgatc gcgctttcgg aggcggcaat gcgttccgac ggcgacggca
    15601 cccgcccgga aggacgtgtt cccggttcga gcgggtgatc gggcggcagg cccggctcga
    15661 tcgtggcgcg cggtggcgcc gcatgctggt ccagaatctc gctgatggcc cttggtgccg
    15721 ccgcggcgag cgccggcagc tggatcggcg cggcctggaa ttcccgcggg gctgtggcga
    15781 aatggctctg cgacgcggcc ggatttggca attcaggctt cggttgcggc gctatcgcga
    15841 tggcaggcgc ggccgccgga gcggatggag gtgccggcgg cgtttccgcc tgcggtgcaa
    15901 ccggctggac gcgaaccgtg cgcaaatcgc cttcgatgat cgccagccga tcgaccacat
    15961 ggccgagcgt gctgtggacg gcttcgaggg attcctgggt atggcggccg gcttcggtct
    16021 gactgaagcg gatatcggac aattcgcgct tgacgatatc gatcacgccg ctgtcagccg
    16081 gctcggccgg ggcgcggctg ctttcggcca ccgcggcaaa cctcgcctgc tggttttcga
    16141 gctggcgcag aatgtcctgc aatccgtcct cgacccgtcc gagatggccg gcgcggtgat
    16201 cggtggacgc ctccaggcgt tcgagcaaat aggacacgcg ctgttcgaga tgggcgaaag
    16261 ccgaggtgtt gtcgttgccg accggcatgc ggtcgaggcg gtccgacagc gcccgcacgg
    16321 cgccctcgag ttgctcggag ctttcgcttg gccccggctg ctcgcggctt tccagcgtca
    16381 gggcgagcgt ggcgatgcgc tgctcgagga tcgtaaacga atcgctgggg ccttcggaac
    16441 gggcaagctg atcgaccttg gacgacaagg tgtggacatc gtcgctgagc cgcgccagcg
    16501 cgtcgttgga cgcgatattg gagacgatgg cgcgaagcgc ggcgatggcg ctttcgagct
    16561 tgtggaccgt ggagggatct tcgctggcgc gcaaaatcag gtcgagcttg gcgccgagat
    16621 tgcggatcgc gtcgtcgtag ccggcgagct gctccgcggg cgtcagggag cgcagcacct
    16681 cgcgaatttc gccgagcgcg cgttcgatgc ctgccagcgc cgaatcgtcg atgccgcttt
    16741 gacgggtgtc gtcaatgcgg cgggacagcg cgcggacttc gctttcgatc gattcgatcg
    16801 cctggcgtgg catcgcctcg gtaatggcgt gacgaatttc ggccaattcg ccgcggaatg
    16861 cggcgatcga ttgctcgatg ccatccggat gctgcagcgc ttcgatctgg ctggtgatct
    16921 ggatcagatg gcgctcgagt gcggaaaagt ccggccccgc cggcgttgcg ggcaccatcg
    16981 gcggcgcgct gcgcggcggc aggaatcgtg gtgcactgtc gagttcgttc tggcgtgcgg
    17041 cgatctctgc caccgcgacg tcgagtgacg cggggctgat cgggggagag ggacgataga
    17101 cctgggcagc cgcgcgctcg accaggtcga actggtcgtc catgagcttg gctgacttgg
    17161 ccggcatggg attggagatc tgcgacagcc gcgcgtcgag gcgcgagatc gcttcgttca
    17221 attgccgggc gacaacagcc gactcgccgc gcacggtgtt gttgcgaagc gcctcgctgt
    17281 gcggcgccgg gcgcgagatc tgctcgatct ggcgcgtgat cgaatccagc cgctggtgaa
    17341 tgtccaggac atcaggggtt tcccggatcg gcggcaccgg cggttgatcg gtggggctac
    17401 caaggttggg aggggtgaaa tcgccgacgg tggaattgag ccagtcgctg agcgacatgc
    17461 cagcgcgccg cgcggcggcc tcggccctgt cgcgaacgga tggatcgatg ccctctacac
    17521 tccacgatag gcgcgaattc atgctctcgt ccggttccgg atccggcgcg tcacttgcgc
    17581 cttcagcctc cccgcgcgtc cctagaattc cgcgcagggc gtctgcctct ggcatcgact
    17641 ttttcgtgat acggtaaaga acgggttaaa gaatcagcca gcaggagctg aaagttgcgt
    17701 ggcgcgcgaa attgaccgtt ggcgggcggc cgccgccggc cgcgccggtc atgttgtcgc
    17761 aatggcggtt cgatagctgc cttacggctg ccttggacgt ccggaaccgt ctaaggtcgc
    17821 ctttatgcaa ctggacgatt gctgcgctgc aaaatcttcc ctaaacgagg cctcgtcagc
    17881 gagccgagag gaacatcatg ccaagctata aagcccccct ggaagacgtc agcttcctgc
    17941 tcaacgatgt gttccagatc gaccgctacg acaatctgcc cggtttcagc gacgcctccg
    18001 ccgacgtgcg ggaggccgtt ctgaacgagg ccgcgaaact cagcgaacag gtattgcagc
    18061 cgctcaatcg cgtcggcgat ctcgagggct gcgtccgcca cgacgatggc agcgtgacca
    18121 ccccgaaagg tttcaaggaa gcctacaggc aggtggcgga aggcggctgg ctcgggctct
    18181 cggcgccaac ggaatatggc gggcagggac tgccggtgac gctgagccag gccgtgagcg
    18241 aattccagat ctcggcgaat atggcgtttt cgatgtatgg cggcctgacc atgggggcga
    18301 cggcggcgct gatggtgcat ggcaagcccg aacagaaggc gatgttcgtg ccgaagatgg
    18361 tggccggcga atggaccggc accatgaacc tgaccgagcc gcaatgcggt accgatctcg
    18421 gcctgctgcg gaccaaggcc gtcaagcagg ccgacggcag ctacaagata tcgggcacca
    18481 agatcttcat ctccgccggc gagcacgatc tcactgaaaa catcatccat ctggtgctgg
    18541 ccagaatcga gggcgcgccc agcggcatca agggcgtgtc gctgttcgtg gtgccgaaga
    18601 ccctggtcaa tgccgacgga tcgctgggtg cgcgcaatgg cgtgacctgc ggctcgatcg
    18661 aacacaagat gggcattcac ggcaattcca cctgcgtgat gaattacgac ggcgccaccg
    18721 gctggctgat cggcgaggaa aacaagggca tgcagggcat gttcgtgatg atgaacgagg
    18781 cgcggctggg tgtcgcggtg cagggactgg cgcaatcgga ggttgcctat cagaatgcgg
    18841 ttgcctatgc ccgcgatcgt ctgcagggcc gctcgctttc gggtgcgaag gcgccggaca
    18901 agccggccga tccgatcatc gtgcatcccg atgtgcgccg cacattgctg acgatcagag
    18961 ccttcaacga ggcggcccgc gcgatggtgg tgtggaccgc gctgaagagt gacgtcgccc
    19021 acaggtcgag cgaccccagg gatcgccagg aggccgacga tcacatgggg ctgatgacgc
    19081 cggtcatgaa gggcgttctg accgatgtgg gattttccaa cgcggtgctg gcgcagcaga
    19141 tgttcggcgg ccacggctat atcgccgaaa ccggcatgga gcagttcgtg cgcgatgccc
    19201 ggatcgccat gatctatgag ggcgccaacg gcatccaggc gctcgacctc gtcgggcgca
    19261 aattgccgcg cgacggcggc cgcgccgtga tggccttctt cggcgaggtg gccgcgttcg
    19321 ccaaggatca cggcgccgac gaagcgatga agccctatgt cgcgccgctc agcacggcgc
    19381 tcggccatct gcagcaggcc accacctggc tgatgcagaa tgcgatgatg aagccggaca
    19441 atgccggtgc cggcgccacc gattacatgc aattgttcgg cctcgtcacc ttcgcctata
    19501 tgtgggcgcg gatggcgaaa gtagcattgg acaagattgc ggccagcggc gcgacgccct
    19561 atctgacaac caagctggtg accggccgct tcttcatgga acggatgctg ccggaaaccg
    19621 cgctgcatct tgcgcgcatc cagaccggaa gcgcgaccac catggaattg gccgcggaag
    19681 cgttctgacc ggagaaatct tcccgacgtc ctttgaatag atccgcgaca tcttccaatt
    19741 cgcctatata ttatgatcat cataccagga gggcgtcatg cctgaggcat atatctacga
    19801 tcacgttcgc acgccgcgcg gccgcggcaa ggccgatggc gcgctgcatg aggtgaccgc
    19861 actggcgctg gcgacggtgc cgctgaaggc gctcaaggag cgcaacaatc tggccgaaga
    19921 cgtcgtcgac gacgtcattc tcggcgtggt cgatccggtc ggcgaggccg gctccgacat
    19981 tgcgcgcttc gcggcgctga aggcgggtct cggcgaagcc gttcccggcg ttcagatcag
    20041 ccgcttctgt gcctcgggcc tcgatgcggt gaatttcgcc gcggcccaga tcatgagcgg
    20101 tcagcatgaa ctcaccatcg gcggcggcgc cgagtcgatg agccgcgtcg gcatcggcgc
    20161 ctccggcggc gcctggccga tggatccctc gatcgcggtg ccggcctatt tcatgccgca
    20221 gggcgtgtcg gccgatctga tcgccaccaa gtacggtttt tcgcgcgacg acgtcgacgc
    20281 ctacgcggtg cggagccagc aacgcgccgc aaaggcgtgg gacgagggcc gcttcaagaa
    20341 ttcggtggtg ccggtgaagg acgtcaacgg cctcaccatt ctcgccaagg acgagcacat
    20401 gcgcccgacc acgaccatgc agtcgctggc gtcgctgcag ccgtcatttg tgaccgtggg
    20461 gcagatgggc ggcttcgatg cggtggcgat ccagtcgcat cccgaagtcg agcgcatcaa
    20521 ttacgtgcat cacgccggca actcgtccgg cagcgtcgac ggcgccggcg cggtgctgct
    20581 cggcagcaag gaggccggtg cgaaatacgg attgaaggcg cgggcgaa
//
LOCUS       DATNOI010000360         2942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_117903,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000360 DATNOI010000000
VERSION     DATNOI010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117903"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VN968_14455"
     CDS             complement(<1..801)
                     /locus_tag="VN968_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXL30473.1"
                     /translation="MSPTAAARTSKASAPSLRDRLKDSSLLREQCYIDGAWAGTPANP
                     VTNPVNGVELAKVPKMSTEEATQAVEAAERAFPAWAKLTAKQRSNILRKWFDLIIANR
                     EDLALILTSEQGKPLAEALGEVDIGGAYVEFFAEEARRVYGETIPAQRPDARLLAIRQ
                     PIGVCGAITPWNFPCSMITRKVSPALAAGCTVVLKPANETPLTALALVALAEKAGVPK
                     GVFNILTGNSSAIGKVLCEHPAVRFVGFTGSTEVGKILYQQAAVGVKKL"
     gene            complement(1017..1334)
                     /locus_tag="VN968_14460"
     CDS             complement(1017..1334)
                     /locus_tag="VN968_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30474.1"
                     /translation="MISTWSEWKRYPRAGRGENIEAPITPGIYEVRYAGTGALHSFGA
                     VDNVAHALSQLAVGSKSWFGRRTEIVPELEYRTCATTSKADAKAAAERMIGRREAYMS
                     GAA"
     gene            1687..2874
                     /locus_tag="VN968_14465"
     CDS             1687..2874
                     /locus_tag="VN968_14465"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXL30475.1"
                     /translation="MQSNARIDQVLRQKSDAREIPGVVAMAATAKEVTYQGAFGKRDL
                     SKDDAMTADSVFWIASMTKAVTAAGAMQLVEQGKLSLEAPIGNVLPDLAAPQVLEGFD
                     SQGEPKLRPARNPITLRHLMTHTAGFCYNLWNGDMVQYLEKTGLPAITTCQNDALKTP
                     LASDPGTRWEYGINIDFVGKAVEAASGKRLDAYLRDHLLTPLGMTDTGFKIGDAQRKR
                     LVGMHARGPDGALAPIPFELEQDPEFHMGGGGLYSTAGDYIRFTQMILNKGRGNGNQV
                     LKAETVATMGQNHMGDLNVTKMISALPDFTNDIDLYPDMAKKWGLSFLINTAKTPEGR
                     SPGSLAWAGLSNTYYWIDPARDVTGVILMQVLPFGDNKCLEAFAGFESGVYAGLDAAQ
                     QAA"
BASE COUNT          584 a          916 c          914 g          528 t
ORIGIN      
        1 gagcttcttc accccgaccg cggcctgctg atacagaatt tttccgactt cggtcgagcc
       61 ggtgaagccg acgaagcgca cggcgggatg ctcgcacagc accttgccga tcgccgacga
      121 attgccggtc aggatattga acacgccctt gggcacgccg gccttctcgg ccagcgctac
      181 cagcgccaat gcggtgagcg gcgtctcatt ggcgggcttc agcaccacgg tgcagccggc
      241 ggccagcgcc ggcgagacct tgcgggtgat catcgaacac ggaaaattcc acggcgtgat
      301 cgcgccgcag acaccgatcg gctggcgtat cgccaacagc cgcgcgtccg gccgctgcgc
      361 cgggatggtt tcgccataga cgcgcctcgc ctcctcggcg aagaactcga cataggcgcc
      421 gccgatgtcg acttcgccca gcgcctcggc cagcggcttg ccctgctccg aagtgaggat
      481 cagggcgagg tcctcgcgat tggcgatgat gaggtcgaac catttgcgca ggatattgga
      541 gcgctgcttg gcggtgagtt tggcccaggc cgggaacgcg cgctcggcgg cttccactgc
      601 ttgtgttgct tcctcggtgc tcatcttcgg caccttcgca agctccacgc cgttgaccgg
      661 attggtcacg ggattggccg gcgtgccggc ccaggcgccg tcgatgtagc actgctcacg
      721 cagcagcgag gaatccttca ggcggtcgcg cagcgatggc gcggaggctt tcgacgtgcg
      781 tgcggcagcg gtcggggaca tggcgttact cctcggatgt ttcttgggat gctgagaagc
      841 aatataggct caaaccagcc gcaatgcatc gtccgcgaac gcgcggctgc tgcaagcaat
      901 tttgagggct aaccaaaatt tggcggctgg gctaatgctt gtcattccgg catggtccca
      961 acgggtcggc actttgcgcg gacccgttgg catcgccccg gaatgacagt ttcaaatcag
     1021 gccgcgccgc tcatataggc ttcgcggcgg ccgatcatgc gctccgccgc ggccttggcg
     1081 tccgccttgg aggtggtagc gcaggtgcgg tattcgagtt cgggtacgat ctcggtgcgc
     1141 cgcccgaacc acgatttgga accgacggcg agctgcgaca acgcgtgcgc gacattgtcg
     1201 acggcaccga atgaatgcag ggcaccggtg cccgcatagc ggacttcata gatgccaggg
     1261 gtaatcggcg cctcgatatt ctcgccacgc ccggcccgcg gataccgttt ccattcactc
     1321 catgtcgaga tcattattgt cccttcgcgg ccttgaaagc cgctatcaat ttgatgaaat
     1381 ttgcagtctg gcagcgtccg gttttgctgc aatctgaacg ctttactacc aaaattggct
     1441 caaaaactcg ccatagaatc gaacatatcg cggatgttcg attcagtcat cgttaatgca
     1501 aggcaattca ctgcaaaaag tgccgagtgt ggccacgaag ccgctccaaa atagccactt
     1561 cctcgccttt ccatagccgc atcacaaggc agcgaccagg acgcgtcaac cgatcacggc
     1621 cttgcgagca acctcggccg ggaggaagat cgttaacttc aagaaaaaca aatcgggagg
     1681 tgcgccatgc aaagcaatgc tcggatcgat caggttctgc gtcaaaaaag cgacgccagg
     1741 gaaatccccg gcgtggtcgc gatggccgcg accgctaagg aagtgaccta ccagggtgcg
     1801 ttcggcaaac gcgacctgtc caaagacgat gccatgacgg ccgacagcgt gttctggatc
     1861 gcttcgatga ccaaggcggt cacggccgcc ggcgcgatgc aactggtgga gcagggcaag
     1921 ctctcgctgg aggcccccat cggcaacgtg ctgccggatc tcgccgcgcc gcaagtgctc
     1981 gaaggcttcg atagccaagg cgagccgaag ctgcgtccgg ccaggaatcc gatcacgctg
     2041 cgccatctca tgacccacac cgccggcttc tgctacaatc tgtggaacgg cgacatggtg
     2101 cagtatctgg agaaaaccgg gctgccggcc atcaccacct gccagaacga tgcgctgaag
     2161 acgccgctgg cgtccgatcc cggcacacga tgggaatacg gcatcaatat cgatttcgtc
     2221 ggcaaggcgg tggaagcggc gagcggcaag cggctcgatg catatttgcg cgatcatctg
     2281 ttgacgccgc ttggcatgac cgacaccggc ttcaagatcg gcgacgcgca acgcaagcgc
     2341 ctggtcggca tgcatgcgcg cgggccggac ggagcgctgg cgccgatccc gttcgaactc
     2401 gagcaggatc cggaattcca catgggtggc ggcgggctct acagcaccgc gggcgattac
     2461 atcaggttca cccagatgat cctcaacaag ggccgcggca acggcaacca ggtgcttaaa
     2521 gccgagacgg ttgcgacgat ggggcagaac cacatgggcg acctcaacgt gaccaagatg
     2581 atttcggcgc tgccggactt caccaacgac atcgatctct atcccgacat ggcgaagaaa
     2641 tgggggctca gcttcctgat caataccgcc aagactccgg agggccgcag ccccggcagc
     2701 ctcgcctggg cgggcctttc caacacctac tactggatcg acccggcgcg cgacgtcacc
     2761 ggcgtcatcc tgatgcaggt gctgccgttc ggggacaaca agtgcctgga agcgttcgcg
     2821 ggcttcgaga gcggcgtcta tgccgggctc gatgccgcgc agcaggcggc ttaggcgcat
     2881 ggcctcttcc cggcgtgatc gaaccgattg cgccgggaaa ttgctcccaa caggcgcggg
     2941 ca
//
LOCUS       DATNOI010000361         7865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1515632,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000361 DATNOI010000000
VERSION     DATNOI010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515632"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VN968_14470"
     CDS             complement(<1..456)
                     /locus_tag="VN968_14470"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL30476.1"
                     /translation="MARMAARKIEKKVETSTTLLEAAKKVLRQNGYAGLSTRGVATAA
                     GVPLSQIHYHFGSKQGMMLALFEYLNAQLLDRQNAMFGDPSLKLSEQWDRACDYLDDD
                     IASGYVRVLQELIAASWSDAVVAQVVRTALMGWVDLVVGVARKAEREFGG"
     gene            636..980
                     /locus_tag="VN968_14475"
     CDS             636..980
                     /locus_tag="VN968_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HXL30477.1"
                     /translation="MRLKSSIWVAAYLRRCQTEGIFGAVRRRGAEEAGAVFVKVALLD
                     GNAMLYAPAPQTVYDDSRPIERIFAPTSPEPVPEASVEERLVKEIRFDPDAWIVETED
                     KAGRHFLELAKA"
     gene            complement(977..1957)
                     /locus_tag="VN968_14480"
     CDS             complement(977..1957)
                     /locus_tag="VN968_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HXL30478.1"
                     /translation="MTTPLLFPGFDGLMTLSRREGVDIRPTLLRVLTDLYVQTSAHND
                     DEERQFVELTSRLIDEVDDATRAVVRARLAIYPGTPAEVLKRLGLQRTSANQVIPLAP
                     EIPAPSSAAAPVKALSEAQLRISSNLSMQPKDAAEITDMFFSASASERALILHNLLET
                     PLKASARIPAARAARAIETLEMAAFAADVENFTLELGEALILPWRIAAQLVNDPGGEP
                     LACAAKALGMPSPVFQRVLLFLNPEVGSSVTQVYRLSRLYDGLHERSALIMLAAWRGS
                     TMAVARAKYRPALYDDDRQRARSAPAQTRPAAQPPGTGGIVRTRSQGSGS"
     gene            complement(2116..3336)
                     /locus_tag="VN968_14485"
     CDS             complement(2116..3336)
                     /locus_tag="VN968_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HXL30479.1"
                     /translation="MQSKNARPKSPGLKWRTRRTGPPVPYWFADKKALDAGYPVKSAN
                     LSTFADNPAKLIERTQRLQSEMLLWLSGQVKPQTQFDGTFKSLLESYERDPESSYQGC
                     KPGVKETYGVYIRRLTDHIGALRIDHVDGRAVKRWFSEWRTDPDGSDHLPRARMVLAV
                     LKAAISFGVVCRQPGCKAFQDVLSELTFETVSSRTFAPTAKQIKAAREAAHAAGAPLR
                     ALLYALIYDTTGREFDFLGEWLPLNYKKPSAIISYRKKWIGPMWSAIDEDLILTIKPT
                     KTEDTTGVEVTFDLSVCPMVMEELALIEPAKRHGPLIINQNTGLPYIYETFRLAWNAD
                     FEAAGMPVGMWCRDMRAGGVTEGGKSGASKDDRRKVAGHAKERQTEKYDRDQLEAFRR
                     TMKSRTGYRAKNGS"
     gene            complement(3595..4152)
                     /locus_tag="VN968_14490"
     CDS             complement(3595..4152)
                     /locus_tag="VN968_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30480.1"
                     /translation="MSSTPDELMGIFRQATLPIGGVVAIARLAAAFQLGNRAHGTALP
                     AATVVKRLTSRPMPECFTVRYDDFGDYAPGRGPLLRRDVKPLNPPVDERPPRVFRSPA
                     GVVSACMTTPGRLACVVPFCRRTTARADFCCDHWCLIDKVRQQAYGRHLRRWRRYGAA
                     TYGAAATRIWRRMVAQAVERAMGIQ"
     gene            complement(4198..4380)
                     /locus_tag="VN968_14495"
     CDS             complement(4198..4380)
                     /locus_tag="VN968_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30481.1"
                     /translation="MKAISLWQPWASLIAACGAKPYETGSFCAAGLANRSIDAIHAAK
                     KINKGAAQFAEELMYG"
     gene            complement(4562..4717)
                     /locus_tag="VN968_14500"
     CDS             complement(4562..4717)
                     /locus_tag="VN968_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30482.1"
                     /translation="MIHRGKNNETMRKQRRLGFYALNKESTLLPNYNPAWHITPAGMA
                     MICEVRR"
     gene            complement(4811..5015)
                     /locus_tag="VN968_14505"
                     /pseudo
     CDS             complement(4811..5015)
                     /locus_tag="VN968_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530375.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
     gene            complement(5701..6732)
                     /locus_tag="VN968_14510"
     CDS             complement(5701..6732)
                     /locus_tag="VN968_14510"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL30483.1"
                     /translation="MDLKRLRTFVVVADLGTVSKAALRLRISQSALSRQISDLEYECG
                     FKLFDRIGRRLFLTTRGEQLLGDCRGVLGQLGSLGERIELLKRGDSGVLKVAAPPQTI
                     ESVLSGFLPRYAERFPNVRVKLTEALGMDQVPLLERGEVHIGIRHDQGVNPCFESLSL
                     PSDDVLAAYRPSLQLGRGGMIDIVLLAPHPLLLLDSGYSVRRLFDAACRVADVEPNIL
                     LESRAPHTLLALAEAGQGIAIIPSVLRTDRYRLKVARVTHRRKPLRDRYVIQWDKRRP
                     MPSYAQNFCAALAAYMRDVLPITRPSQGSGLSALSASGPSRRSRDSSVGPLSKGDRTA
                     LRNREDGRP"
     gene            6915..>7865
                     /locus_tag="VN968_14515"
     CDS             6915..>7865
                     /locus_tag="VN968_14515"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXL30484.1"
                     /translation="MKIRLLIFLSALPFMLLGMTASLRAQQQETGPAARRVIIFVWDG
                     LRADDLTPEIAPNYFALARSGVVFADHHAVYPTFTMMNSASIATGAYPGVHGFYGNVV
                     YAPSAKGRNAKGADIDFSAPAFIEDFGVVEAVRDSYQGRLTLVSTMLQAAQAKGLTTA
                     AIGKFGAAFIQDYRRGGIILDEDAAIPLGFAKELQHAGYRLPRNSSNAYDAGALMLAK
                     DNGDPTAPIPIQRLKDAQTSNPLDCSGALSRPGFAYLTDVFVNYILPNKKPDLTIFWS
                     KEPDATNHAYGPGTCNSIDATRMNDEILGRVVEELRQLGWE"
BASE COUNT         1483 a         2347 c         2468 g         1567 t
ORIGIN      
        1 gccgccgaac tcgcgttcgg ccttgcgcgc aacgccgacg acgagatcga cccatcccat
       61 gagcgcggtg cgaacgacct gggcgaccac ggcgtcggac cagctcgcgg cgatcagttc
      121 ctgcaggacg cgcacgtagc cagaggcgat gtcgtcgtcg agatagtcgc aggcccgatc
      181 ccattgctcc gacagcttga gactggggtc gccgaacatg gcattctgac ggtcgagcag
      241 ctgcgcgttc aggtattcga acaacgccag catcatccct tgcttggatc cgaagtgata
      301 gtggatctgg cttaagggga cgccggccgc cgtcgccacg ccgcgggtcg ataaaccagc
      361 gtagccgttc tggcgaagca ccttcttggc ggcttcgagc agcgtcgtgc tggtctcgac
      421 ctttttctcg attttcctgg cggccatgcg ggccatcttg gatctcctgg gcgcgcgagg
      481 gtgatctcaa ggccgggatt ggcataattc ggtcgaccga ccgagtcaat gttatcgtag
      541 tatagctcac ggatttgtgc gccgacatcg atcgaatgga ggagttcgtg aaggcgcgct
      601 ggcttcggct ttggcgcggg cctatcgtca ggctcatgcg attgaaatcc agcatatggg
      661 tggcggccta tctgcgccgc tgccagaccg agggaatatt tggcgctgtg cgccggcgcg
      721 gcgcggaaga ggccggcgcg gtgttcgtca aggtggcgct gctcgacggc aacgccatgc
      781 tgtacgcgcc ggcgccgcag acggtctatg acgacagccg gccgatcgag cgcatctttg
      841 cgccgacatc gccagagccc gtgccggaag cgtcagtgga agagcgcctc gtcaaggaaa
      901 tccggttcga cccggatgcc tggatcgtgg agaccgagga caaagcaggg cggcattttc
      961 tcgaactggc gaaggcttaa cttcctgaac cctgcgaccg ggtgcgcaca atgccgccgg
     1021 tgccaggcgg ttgcgctgcc gggcgggttt gggcgggcgc cgagcgggcg cgttggcgat
     1081 cgtcgtcgta aagcgcgggc cggtatttgg cgcgggccac cgccatggtc gaaccgcgcc
     1141 acgcggccag catgatgagg gcagatcgct cgtgcaatcc gtcatacagg cgcgacagcc
     1201 ggtagacctg cgtgaccgag gatccgacct caggattgag aaacagcaac acccgctgaa
     1261 aaaccgggct cggcatgccc agggccttgg cggcgcaagc cagcggctcg ccgccgggat
     1321 cgttgaccag ctgtgccgct atccgccacg gcaatatcaa ggcttcgccg agttccagcg
     1381 tgaagttttc gacatcggcg gcgaatgccg ccatctccag ggtctcgatg gcgcgcgccg
     1441 cgcgtgcggc gggaattcgc gcggaagcct tgagcggcgt ctccagcaga ttgtgcagga
     1501 tcaaggcccg ctcgctggcg ctggcgctga agaacatgtc ggtgatctcg gcggcgtcct
     1561 ttggctgcat cgataaattc gatgatatcc gcaattgcgc ctcgctcagc gccttgaccg
     1621 gcgccgccgc cgaggacggc gcgggaatct cgggcgccag gggaatgacc tggttggcgc
     1681 tggtgcgttg cagcccgagc cgtttcagga cttcggccgg tgtgcccgga tagattgcga
     1741 gccgcgcccg aaccaccgca cgggtggcgt catcgacctc atcgataagg cgggaggtga
     1801 gctcgacaaa ctgccgctct tcgtcgtcgt tatgtgcgct ggtctggacg tagagatcgg
     1861 tcagtacgcg cagcaaagtg ggtcgaatat ccacgccctc tcggcgggag agcgtcatca
     1921 ggccgtcaaa gcccggaaac aggagcggtg tggtcatgtc agaggtacgc gactgagaaa
     1981 tactcgcgcc agtctaccgg cgttgcttta acagctcgtt aacattaacg aattcatgca
     2041 atctgcaagc gattgtttct actgcgaaat ttttgccgcg tggtcccatg gtcccggcgt
     2101 ggtcctgagc tttgtttacg acccgttctt ggcgcgatat ccggtgcggg atttcatggt
     2161 ccgccggaac gcctcgagct ggtcgcggtc gtatttctcg gtctgtcttt ccttggcgtg
     2221 gccggcgacc ttgcgccggt cgtccttgct ggcgcctgac ttgccgccct cggtgacgcc
     2281 gccggcgcgc atgtcgcgac accacatgcc gaccggcata ccggccgcct cgaagtcggc
     2341 gttccaggca agccgaaagg tctcatagat gtagggcagg ccggtgttct ggttgatgat
     2401 cagcgggccg tggcgcttgg ccggctcgat cagcgccagc tcttccatca ccatcgggca
     2461 caccgaaagg tcgaacgtca cctcgacgcc ggtggtgtct tcggtcttcg tcggcttgat
     2521 cgtcaggatc aggtcttcgt cgatcgccga ccacatgggg ccgatccact tcttgcgata
     2581 gctgatgatc gcgctcggct tcttgtagtt caatggcagc cattcgccga gaaagtcgaa
     2641 ttcgcggccg gtggtgtcat agatcagtgc atagagtaac gcgcgaagcg gggcgccggc
     2701 ggcatgcgcg gcttctcgcg cggccttgat ctgctttgcc gtcggcgcaa aggttcgaga
     2761 cgagacggtc tcgaaggtca gctccgacag aacgtcctgg aatgctttgc agccaggctg
     2821 ccggcagacg acgccaaacg atattgccgc cttcaagact gcgaggacca tccgcgcgcg
     2881 cggcaaatgg tcggacccgt cggggtcggt gcgccattcc gaaaaccagc gtttcacagc
     2941 gcgaccgtca acatgatcga tgcggagcgc gccgatatga tcagtcaggc gccggatgta
     3001 gacgccatag gtttccttca cgcccggctt gcatccctga tagctgctct ctggatcgcg
     3061 ctcgtaggac tccagcagag acttgaaggt cccatcaaac tgggtctggg gcttcacctg
     3121 gcccgacagc caaagcagca tctccgattg cagacgttgc gtgcgctcga tcagcttggc
     3181 ggggttgtcg gcgaaggtcg aaaggttcgc cgacttcaca ggatagccgg cgtccagtgc
     3241 cttcttgtca gcgaaccagt atgggaccgg aggcccggtc cggcgggttc gccatttgag
     3301 acccggactt ttcgggcgcg cgtttttgct ttgcatggaa tgtaccttct actccatttg
     3361 gggcgagcgg gaccgcggtc gacaggccat actgcgtgtc aaggaacgcc ttcacggctg
     3421 gaacataacg accgccccac acggggctga tcttcggcat tccgtcccgt tcatgcaagg
     3481 cggccaagcc ggcgaactcc ttgcgtcgct catagccaag ggcggcttcc ccgatatcct
     3541 cgtctgaggc gaataggtgc aacttgtccc aggtaagggg atttttggcc gtcattactg
     3601 gatccccatc gcacgttcga ccgcctgagc gaccatgcgg cgccagatcc gcgtcgcggc
     3661 cgcgccgtat gtggctgcac cgtatcgccg ccagcgccgc agatggcggc cgtaggcctg
     3721 ctggcgcacc ttgtcgatca agcaccaatg gtcgcagcag aagtcggcgc gcgcggttgt
     3781 ccgccggcaa aacgggacga cgcaggcgag gcggccgggc gtcgtcatgc aggcgctaac
     3841 cactcctgcg ggagatcgaa aaacccttgg cggcctttca tcgaccggtg gattgagcgg
     3901 cttgacgtcg cgccgcaaca agggcccccg gccaggcgca tagtcgccga agtcgtcata
     3961 gcgaaccgtg aagcattccg gcatcgggcg gctggttagc cgcttgacca cggtcgcggc
     4021 tggcagcgcc gtaccatgcg cacgatttcc taactggaac gctgcagcaa gccgggcgat
     4081 cgcgacaacg ccgccgatcg gcaacgtagc ctgtcggaat atgcccatca actcgtcggg
     4141 ggtgctggac atcgttgctt cgagtctgcc ggcgaggtcg aacccgccat ccttgtgcta
     4201 gccatacatc agctcttcgg cgaattgagc ggcaccctta ttgattttct tcgcagcatg
     4261 aatcgcatcg attgacctat tagcgaggcc ggcggcgcaa aagctgccgg tctcatacgg
     4321 cttcgcaccg caggcggcga tcagcgaggc ccatggttgc caaagcgaga tcgctttcat
     4381 gattgtcctt tcgcatcggt cggcattttg cggtcggatc gcgttccgac tggtcaccgc
     4441 ggtgaagccg tcctcgaatt tgacgaggct gccattcatc ttgccgcgcg cccccacagc
     4501 gcaggtgtgg cctttgcgct catccggttc cagtgataga catagcggaa gtctggttgc
     4561 gtcatcgacg cacctcgcag atcatcgcca tgccggccgg cgtgatatgc caggcggggt
     4621 tatagttcgg cagcaacgtc gactctttat tgagcgcata aaatccgaga cgccgctgct
     4681 ttcgcatggt ctcattgttc ttgccgcgat ggatcacgcc gccttgctgg atcgtttcgt
     4741 gaaagatgat tgcgtgttcc agtggcgacc ggattccgca gcttccgcgc ggccttgatg
     4801 aattgctgga tgttttccgc tgatggacgt caggcgacgg cgcgcgattg atggctttcg
     4861 ctttggaggc ctcgcgtcgc atttcgaccc aagcctcgac ttcggtccac acaacgcaac
     4921 gggacgttag gtagacgcgt tgcggaaact cgcctcgttg ttccatgtcg tagatcgtgg
     4981 tgtggaacaa tctcgcggaa ttgctgcctg cgaatagttc gtctggtcgg gtactggttg
     5041 cgggcgactc atggcgggtc cttcagttat gaaaccgagg acgataaagc cgatagtctt
     5101 cacaggttgg aagtctccaa agcaggtagt agaagaaacg gcgacagcag cgcgcaaaag
     5161 ggacggcgtc tgccgggcgg ctgttgaaag attggcccca aaggacgctt gtcgaaatcg
     5221 atagtcgcgc tccgttcgtg tgccattctc tcaagacgga cgatctggcc agcccgatcg
     5281 cggcgcaaca tcctcgaact ttgggcggcg atgttgctga aggggtgtcc cgtcaaccat
     5341 atggttttgt tgccgaaaac agctttgaag cctgcagatc tacggttcta accacgcgca
     5401 gccggtaggg ttgggcgcag acgttgaatg agaacccgta ccgcgagttc gaatctctct
     5461 cgctccgcca gtaagtctct gatattacag tagaaaatgt ctaagactca attgtgccgt
     5521 cacttccgcc ggttagcgac cgtagttgcg ggggttggcc actgagagag cgaattcctc
     5581 gcggtttcca cgttcattgt cggtcgtttc tcagaaggcg actttgccag tggctttctc
     5641 tgcgaatgat ggtgctgatt acaggtcggg cgtgcacagc ctacgatctt gcactacgcc
     5701 tcatgggcgg ccgtcttccc gatttcgcag ggcggtccgg tcacccttcg atagcgggcc
     5761 taccgatgaa tcccgggatc gacgtgatgg gccagaagca gacagcgcgc ttagcccgct
     5821 accctgcgac gggcgcgtaa tcggcaggac gtcgcgcatg tacgctgcga gcgccgcgca
     5881 aaagttctgg gcatagctcg gcatgggacg ccgcttgtcc cattggatga cgtaacggtc
     5941 tcgcagcggt tttcgccgat gagtgactcg agcgactttc agcctgtaac ggtcggtccg
     6001 gagcacggac gggatgatgg cgatcccttg cccggcttca gcgagcgcga gcaatgtgtg
     6061 cggcgcgcgg ctctcgagca gaatattggg ctcgacgtcg gcaacacggc aggccgcatc
     6121 gaacagcctg cgaactgagt agccggagtc cagcagcagc aagggatggg gtgcgagcaa
     6181 aacgatatcg atcatgccgc cgcggcccag ctggagcgac gggcggtacg cggccaaaac
     6241 gtcatccgac ggcaatgaga ggctctcgaa gcaggggttc acgccttgat catgcctgat
     6301 gccgatatga acctcgccac gttccagcag gggcacttgg tccatgccaa gagcctccgt
     6361 caacttcacg cgcacgttgg ggaagcgttc ggcgtatcgc ggcaggaatc ccgacaagac
     6421 gctctcgatc gtctgcggtg gtgcggccac cttaaggacg ccggagtctc cacgtttgag
     6481 cagctctatc cgttcgccaa gcgatcccag ctgacccagc actccgcggc aatctccgag
     6541 cagctgttcg cctctggtcg tcaggaaaag gcggcgaccg atacggtcaa aaagcttgaa
     6601 cccgcattcg tattcgagat cactgatctg ccgcgataaa gcagactggc tgatacgtag
     6661 acgcagcgct gccttggaaa ctgtgccgag gtcggccacc acgacgaagg tccgcaggcg
     6721 cttcaggtcc atcctgctta tctccaaaac gcataaggaa tagaacaatt atgtatttga
     6781 cgcaaacgca ggcgcgctca tagcctgccg taacacgcgt taaatcgatg gtctctcttg
     6841 cgaaatccag gcggcggaca gtccggactt ggatcgggta atcgacgctc gcagcgctgc
     6901 ccaggaggcg aaccatgaaa atacggttgc ttatttttct gagcgctctc cccttcatgc
     6961 tgctcggaat gacagcaagt ctgcgagcgc agcaacagga gacgggcccg gctgcacgac
     7021 gagtcatcat cttcgtgtgg gatgggcttc gagctgacga cctgactccg gaaattgcgc
     7081 cgaactattt cgcgcttgcg cgatccggcg tcgtctttgc agaccaccat gccgtctatc
     7141 cgaccttcac gatgatgaat tccgcttcca tcgccaccgg cgcctatccg ggcgtgcacg
     7201 ggttctacgg caacgtggtg tatgcgccgt ctgcgaaggg aaggaacgcg aagggagccg
     7261 atatcgattt ttccgcgccg gcgttcatcg aggatttcgg cgtggtcgaa gccgtgcgcg
     7321 actcgtacca ggggaggctg actctggttt cgacgatgtt gcaggctgcg caagcaaaag
     7381 ggctgacaac ggcggccatc ggcaagttcg gagccgcctt catccaggat tacaggcgcg
     7441 gcggaatcat cctcgatgaa gatgccgcga ttccgctcgg tttcgccaag gaactgcaac
     7501 acgccggcta ccggcttccg cggaacagca gcaacgcata tgacgcgggt gcgttgatgc
     7561 tcgctaaaga caacggcgac cctacagctc caatccccat ccagcgccta aaggatgcgc
     7621 agaccagcaa tccgctcgac tgcagcgggg cgctatcccg gcccggcttc gcttacctga
     7681 ctgacgtgtt cgtgaattac atcctgccca acaagaagcc ggacctgacg atcttctggt
     7741 cgaaggagcc agacgctacg aatcacgcgt acgggcctgg aacgtgcaac tcaatcgacg
     7801 cgaccaggat gaacgacgag atcttgggcc gggtcgtcga agaactgcgc cagctcggct
     7861 gggaa
//
LOCUS       DATNOI010000362         5365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1502143,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000362 DATNOI010000000
VERSION     DATNOI010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502143"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="VN968_14520"
     CDS             complement(<1..1377)
                     /locus_tag="VN968_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HXL30485.1"
                     /translation="MNKTTQTVESAAAVGAFHIEDVYPLIDGGRFAVKRIVGERIEVW
                     ADVYRDGHNVVAAALLWRRERDREWRREPMTHHGNDRWVGSFVPDQLGHHVYAIEAWT
                     DEFATWRHGFELKQKAGADLTLDAIEGAGMLTKAQPGGQDAAAVILKQCEDFLQTGDA
                     APLLTAALKDAMAESQLRPDLTRSQLFPLVVDRPRARTGAWYEMVPRSQGQIPGQHGT
                     FRDSIARLPDIAAMGFDVVYLTPIHPIGRTNRKGRNNAVTSVECDPGSPYAIGAAEGG
                     HDAVHPELGTLKDFREFVAACTRLEMEVALDFAIQCSPDHPWLKQHPQWFRRRPDGSM
                     RYAENPPKKYEDIVNPDFSSEDAGALWNALRDVVLFWIEQGVKIFRVDNPHTKPFRFW
                     EWLIREVQHRHPDAIFLAEAFTRPKLMKGLAKLGFTQSYTYFTWRTQKWELEQYLTEL
                     TGYPERDYY"
     gene            1578..3536
                     /gene="malQ"
                     /locus_tag="VN968_14525"
     CDS             1578..3536
                     /gene="malQ"
                     /locus_tag="VN968_14525"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HXL30486.1"
                     /translation="MDLYTKAKNLGIQTEFLDGQGHLHVTDAEALKIILDALPVHKPY
                     RFLEQAVVIRSGRPARTELSDAATFPLRWKILAGLKVIAAGETHDRLIGWPVDLPAGS
                     YRLHLTDASSFTEEAPLIVAPPRAFEGDFDRCWLLAAQLYGVRSTRNWGIGDFTDLSG
                     LIELAVHLGADGVGLNPLHALFDDRPADCSPYSPNSRLFLNALYIDVEKLPEFQRGVV
                     ATSSKDIARLRASDTVDYIGIAELKWRGLRRAFETFKADSDNARRRDFEKFRAERASL
                     LSRFACFEVLRHKFNKPWWEWPEQWRKPDDNKLGKLRAGTDATEIEFVEFVQWTADRQ
                     LQSCRDLAARRGMKVGLYLDVAVGVQADGFDAWNEQVAISRHLAVGAPPDSLNTSGQN
                     WGLAGFNAAGLEIKSFEPYRDMLRASVRYAGAIRLDHVLGLKRLYLVPHGFAADNGVY
                     VQMPFEALLAATALESVAHRCVVIGEDLGTVPEGFREQIADWGIWSYQVMMFERDDQG
                     KFRGIDHYSPNALVTFNTHDLSTYAGWRSFSDLALKRSLGIDPGESDDARWQALAMLT
                     EVLRQYGIDRHDLNAVVGFLARTKSRLLAISLEDLLGVIDQPNIPGTVNEHPNWRQRL
                     PLALDEIASAIDVPALKTAIAERTRVAT"
     gene            3533..>5365
                     /locus_tag="VN968_14530"
     CDS             3533..>5365
                     /locus_tag="VN968_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HXL30487.1"
                     /translation="MRRWTSLAGSKNLPQKIEDYGLIGDCETAALVGRDGSIDWLCWP
                     AFDSDACFAALLGTHRNGRWLISPAEEVTKSARRYWDNTLILETRFETTNGVVALIDF
                     MPPRGHASDVVRLVRGVTGKVKLQMQLVIRFGFGTDIPWVKRTEDGALLAIAGPDMTV
                     LRTPIETRGENLTTVAGFEVNEGETIPFVLTYGRSHLPLPAPINPAQALQETEDFWTE
                     WCSRCTYEGANRDLVMRSLITLKALTYRPSGGIIAAPTTSLPEKLGGTRNWDYRFCWL
                     RDATFTLLALMNSGYTEEALAWHNWLLRAAAGWPANMQIMYGIMGERRLLEWEADWLS
                     GYEGAQPVRIGNAAHAQLQLDVYGELIDAFYQSRMAELRLDQGSWALGRAVLEHLEEV
                     WDQPDHGIWERRGVRKHYVLSKVMTWVAFDRAIKGAEKFGFEAPLDRWRALRDAICRD
                     VCENGFDGQQNAFVESYGSQLLDASILLLPAVGFLPASDPRVRGTIAAIEKNMMCDGF
                     VLRHDPREVSEERQPIEGAFLACTLWLADAHVLAGEVARAQALFERVVAVANDLGLLA
                     EEFDPGMARQTGNFPQALTHIALINTAQNLSHAKKPVEKPAVQRS"
BASE COUNT         1002 a         1651 c         1668 g         1044 t
ORIGIN      
        1 atagtaatcg cgctccggat agccggtgag ttcggtcagg tactgctcca gctcccactt
       61 ctgcgtccgc caggtaaagt aggtgtagga ttgcgtgaag ccaagcttgg cgaggccctt
      121 catcagtttg ggccgcgtaa aggcttcggc gaggaagatg gcatcgggat ggcggtgctg
      181 aacctctcgg atcagccatt cccagaatct gaagggtttg gtatgcgggt tgtcgacccg
      241 gaaaatcttc accccctgtt cgatccagaa cagcactaca tcgcgcaacg cgttccacag
      301 cgcgccggca tcctccgacg aaaagtcggg attgacgatg tcctcatact tcttcggcgg
      361 gttttccgca tagcgcatcg agccgtccgg ccggcgcctg aaccattgcg gatgctgctt
      421 gagccagggg tggtccggcg agcattgaat ggcgaagtcg agcgcgactt ccatctcaag
      481 ccgggtgcac gccgcaacga actcacggaa atccttcaac gtgcccaatt ccggatgcac
      541 cgcatcgtgg ccgccttcgg cggcgccgat cgcgtacggg cttccgggat cgcattccac
      601 ggaagtcacc gcgttgttgc ggcccttgcg gttggtgcgg ccgatcggat gaatcggcgt
      661 gagatagacc acgtcaaaac ccatcgcagc gatgtcaggc aatcgagcga tgctatccct
      721 gaatgtgccg tgctggcccg gaatctgacc ctggctgcgc ggcaccatct cgtaccaggc
      781 gccggtccgc gcccgcggcc ggtccaccac caatggaaac aattgcgatc gcgtcaggtc
      841 gggccgcaac tggctctccg ccatggcgtc tttcaatgcg gcggtgagca ggggtgcggc
      901 atcgccggtc tggagaaaat cctcgcattg cttcaggatt acggcggccg cgtcctgtcc
      961 gccgggctgt gccttggtca gcatgcccgc gccctcgatc gcatcgaggg taagatcagc
     1021 gccggccttt tgcttaagtt caaagccgtg ccgccaggtg gcgaattcgt cggtccaggc
     1081 ctcgatcgca tagacatgat gtccgagctg gtccggcacg aaggatccca cccagcggtc
     1141 gttgccgtgg tgggtcatcg gctcgcgccg ccattcccgg tcgcgctcgc gccgccacaa
     1201 cagcgccgcc gccacaacat tatggccgtc acgatagaca tccgcccaga cttcgatgcg
     1261 ctcaccgacg atccgcttca cggcaaagcg gccgccatcg atgagcggat aaacatcttc
     1321 aatatgaaaa gcgccgacgg ccgcggcgct ctcgacagtt tgagttgtct tgttcacggt
     1381 gatgccattg acatggatat aacgtcccgt ttcgattgcg ggaaaggaga cgctgcgaac
     1441 ccctatagaa agccgctggc cgggcattgg ttcccgggga acgccggcgc ccccagagag
     1501 ttaaacacgg ttatcctctt actgcatcta gagaatttcg cgacgcccct aaccgttgcg
     1561 tgcaaacagc gtgccgaatg gacctttaca ccaaagctaa aaatctcgga atccagaccg
     1621 aattcctgga cggccagggt caccttcatg tgaccgacgc tgaggcactg aagatcattc
     1681 tcgatgccct tccggtacac aaaccctacc gatttctcga gcaggcggtg gtgatccgct
     1741 ccggccgacc cgcgcgaacc gaactgagcg acgctgccac gtttccgttg cgctggaaaa
     1801 tccttgcggg tcttaaggtt atcgcggcgg gcgaaaccca tgaccgcctc atcggctggc
     1861 ccgtggattt gcctgccggt tcataccggc tgcacttgac cgatgcctcg tcattcactg
     1921 aagaagcgcc attgatcgtg gctccgccca gggccttcga gggcgatttc gaccgctgct
     1981 ggctgttggc ggcccagctc tatggcgtcc gctcgacacg caattggggc atcggggact
     2041 tcaccgatct gtcgggtttg atcgagctcg cagtccattt gggggccgac ggcgtcggcc
     2101 tcaatccgct gcacgcatta ttcgacgacc gccccgccga ttgcagtccc tattcgccga
     2161 acagccggct gttcctgaat gcgctctata tcgacgtcga aaaactcccg gaatttcagc
     2221 gcggcgttgt cgccacgagc agcaaggaca tcgcccggct gagagcgagc gataccgtcg
     2281 actacatcgg cattgccgag ctgaaatggc gcgggcttcg ccgggcgttc gaaacattca
     2341 aggccgattc ggacaatgca cgccggcggg attttgaaaa atttcgcgcc gagcgcgcat
     2401 cgctgctgtc gcggttcgcc tgcttcgaag tgctgcggca caaattcaac aagccgtggt
     2461 gggaatggcc ggagcaatgg cgaaagcccg acgataacaa actcggcaaa ctgcgtgcgg
     2521 gcaccgatgc aaccgagatc gagttcgtgg aattcgtgca atggaccgcc gaccgccagt
     2581 tgcagtcctg ccgcgatctc gcggcccggc gtggcatgaa agtcgggctc tacctcgatg
     2641 tcgccgtcgg cgtgcaagcg gacggattcg atgcctggaa cgagcaggtc gcgatttccc
     2701 gccatctggc cgtcggtgcg ccgccggact ctctcaacac atcgggccaa aactggggtc
     2761 tcgccgggtt caacgccgcc ggtctggaaa tcaagtcctt cgaaccctat cgcgacatgc
     2821 ttcgggcttc ggtgcgttac gcgggcgcga tccggctcga tcatgtgctc ggcctgaagc
     2881 ggctctatct cgtgccgcac ggctttgccg ccgacaacgg cgtctatgta caaatgccgt
     2941 tcgaggcgct gctggcggcg acagcgctgg aaagcgtggc gcatcgttgc gtggtgatcg
     3001 gcgaggatct cggcaccgtg ccggaaggct ttcgcgagca aatcgccgat tggggcatct
     3061 ggtcgtatca ggtgatgatg ttcgagcgcg acgatcaggg caagtttcgc ggcatcgacc
     3121 attattcgcc aaatgctctg gttaccttca acacccacga tctctccacc tatgccggct
     3181 ggcgctcgtt cagcgatctc gcgctgaaac ggtcgctcgg gatcgatccc ggcgaaagtg
     3241 acgacgcgcg ctggcaggcc ctggccatgc tgaccgaggt cttgcgtcag tacggcatcg
     3301 accggcacga ccttaacgcc gtggtgggct ttctggcgcg gaccaaatcc cgcctgcttg
     3361 caatctcgct ggaagatttg cttggcgtga tcgatcagcc caacattccc ggcaccgtga
     3421 acgagcatcc gaactggcgg cagcggctgc cgcttgcgct cgacgagatt gcctcggcga
     3481 tcgacgtgcc cgcgctcaaa acggccatcg cggagcggac ccgggtggcg acctgaggcg
     3541 ttggacatct ttagcgggga gcaagaactt gccccaaaag atcgaggact atggactgat
     3601 cggggattgc gagaccgcgg cactggtcgg ccgcgacggc tcgatcgact ggctgtgctg
     3661 gcccgcgttc gattccgatg cctgctttgc ggccttgctc ggcacgcaca ggaacggccg
     3721 ctggctgatc tcacctgcgg aagaggtcac aaagtccgcc cgccgctatt gggacaatac
     3781 actgatcctg gagacgcggt tcgagaccac caatggcgtt gtcgccctga tcgatttcat
     3841 gccgccgcgc ggccacgctt ccgacgtcgt ccggctggtg cgcggcgtta ccggcaaggt
     3901 gaagctgcag atgcagctgg tgatccgctt cggcttcggc accgacatcc cctgggtcaa
     3961 gcgaaccgaa gacggcgcgc tgctggcgat tgccggaccg gacatgaccg tgctgcggac
     4021 gccgatcgag acccgaggcg aaaacctgac taccgtggcc ggtttcgaag tcaacgaagg
     4081 cgagaccatt cccttcgtgc tcacctatgg ccgttcgcat ctaccgcttc ccgcaccgat
     4141 caatcctgcg caagccttgc aggaaaccga agatttctgg accgagtggt gcagccgttg
     4201 cacctatgag ggcgccaacc gcgacctggt gatgcgttcg ctgatcacgt tgaaggcgct
     4261 gacctacagg ccgagcggcg gcattatcgc ggcgccaacg acctcgctgc cggaaaagct
     4321 gggcgggacg agaaactggg actaccgctt ttgctggctg cgcgacgcca cctttacgct
     4381 gttggcgctg atgaactccg gttacaccga ggaagccttg gcctggcata attggctgct
     4441 gcgcgctgcg gcaggctggc ctgcgaacat gcagatcatg tacggcatta tgggcgagcg
     4501 gcgccttttg gaatgggagg ccgactggtt gtccggctat gaaggcgcgc agcccgttcg
     4561 gatcggcaat gcggcacatg cccaactgca actcgatgtt tacggtgagt tgatcgacgc
     4621 cttttatcag tcgcggatgg cggagctgcg cctcgaccaa ggctcctggg cgttggggcg
     4681 ggcggtgctt gagcatctgg aggaagtatg ggaccagccc gatcacggca tctgggagag
     4741 gcgcggtgtt cgaaagcact acgttttgtc gaaagtgatg acatgggtcg cgttcgatcg
     4801 cgccatcaag ggcgccgaaa aattcggatt tgaagcgccg ctcgatcgtt ggcgggcttt
     4861 gcgcgacgcc atctgccgcg atgtctgcga aaacggcttt gatggtcaac agaatgcgtt
     4921 cgtcgagtcc tacggctcgc aattgctcga tgccagcatc ctgctattgc cggcggtggg
     4981 ctttctgccg gcgtcggacc cgcgggtgcg cggcaccatc gcggccatcg aaaaaaacat
     5041 gatgtgcgac ggatttgtgc tgcgccacga tccccgcgag gtctccgagg aacggcagcc
     5101 gatcgaaggc gcgtttctcg cctgcaccct ttggctcgcc gacgcccatg tactggccgg
     5161 cgaggtcgcc agggcgcagg cgctgttcga gcgagtggtc gcggtcgcga acgatctggg
     5221 cttgctggcg gaggagttcg atcccggtat ggcacgtcaa accgggaatt ttccgcaggc
     5281 gctgacgcat atcgcactga tcaataccgc ccaaaacctc agccacgcca agaaaccggt
     5341 cgagaagccg gcggtgcagc ggtcg
//
LOCUS       DATNOI010000363         5451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1471931,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000363 DATNOI010000000
VERSION     DATNOI010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471931"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..499
                     /locus_tag="VN968_14535"
     CDS             98..499
                     /locus_tag="VN968_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HXL30488.1"
                     /translation="MIGLVLVTHGRLADEFKAALEHVMGPQKQIEAVTIGAEDDADLC
                     RSDIIEAVNRVDSGDGVAILTDMFGGTPSNLAISCMSRPKVEVLAGINLPMLVKLAKV
                     REERSLPDAIAMAQEAGRKYVTIASRVLAGK"
     gene            496..825
                     /locus_tag="VN968_14540"
     CDS             496..825
                     /locus_tag="VN968_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HXL30489.1"
                     /translation="MSDDAASPAPDAGPGIPQGALSRELPIINKRGLHARASAKFVQM
                     VERFDAEVWVTRGNETVGGTSIMGLMMLSAGPGTSIVVSAAGPEAQAAIDAITELVAS
                     KFNEEST"
     gene            complement(987..2609)
                     /locus_tag="VN968_14545"
     CDS             complement(987..2609)
                     /locus_tag="VN968_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXL30490.1"
                     /translation="MSTASFLPAASRARRRPSIRRLAAWLAACASDPKTSLWLVIGFG
                     VAHAVLWTFILINLKAAQDVHMDVAEAFAWGQKFQLGYGKHPPLAGWVAGLWFKVFPV
                     ADWATYALAMATLGCGLVISWLIALRVVDRRRAFFVVVMLALYPIFNFKGFKYNPDLL
                     QLVTLPLVVLAYLNAFEKRGVRSGLWLGLAGALALMTKYWVLTMIGAIGLAALIHPDR
                     LKFLRSPAPWVAIVTLVIAMIPHLVWLKEADYVPLTYAGDVYSLSSRAQSVQLVLGYI
                     GHNLALLAAPVALAAMALAWPSRRAKLGWQPSALFAQAWSRAENRSVNRSQALNIWII
                     QIVVAIGPPLGGLIFTIYMKTDWGISLFFLTPLALVAIPSLRIHRITLFRIVAIWLTV
                     TLAALAASPYIATEEMAVNPNGAATYGARSQLARELTQAWHSRFNTRWAVVAGTTEVG
                     EPMTFYSADHPAPFTPGELWSSGLTSLEEARRLGFIGICDTTDGRLPACEAWMRENGK
                     DAEQLAITTQRFFRGHPGPAISWKIYIVPPAK"
     gene            2723..4528
                     /gene="lepA"
                     /locus_tag="VN968_14550"
     CDS             2723..4528
                     /gene="lepA"
                     /locus_tag="VN968_14550"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HXL30491.1"
                     /translation="MTSIPISNIRNFAIVAHIDHGKSTLADRLIQTTGGLAAREMKEQ
                     VLDSMDIERERGITIKAQTVRLTYPAKDGKTYILNLIDTPGHVDFAYEVSRSLAACEG
                     SLLVVDASQGVEAQTLANVYHALDAGHEIVPVLNKIDLPAAEPDQVKRQIEDVIGLDA
                     SDALMISAKTGLGIDDVLEAIVHRLPPPRGDPDASLKALLVDSWYDVYLGVVVLVRIV
                     DGVMKKGQRIRMMGTNAAYDVERVGFFTPKMQQIDELGPGEIGFITAAIKEVADTRVG
                     DTITDDRKPVTDMLPGFKPAIPVVFCGLFPVDANDFESLRAAMGKLRLNDASFSFEME
                     TSAALGFGFRCGFLGLLHLEIIQERLQREFDLNLIATAPSVIYKMHLTDGQVIEIHNP
                     IDMPDVVKIAEIEEPWIEATILTPDEYLGSVLKLCQDRRGAQKELTYVGNRAMVKYEL
                     PLNEVVFDFYDRLKSVSKGYASFDYHLTDYKPADLVKMQILVNNEPVDALSMLVHRQR
                     AEGRGRAMVEKMKELIPPHMFQIPIQAAIGGKVIARETVRALRKDVTAKCYGGDITRK
                     RKLLEKQKEGKKKMRQFGKVDIPQEAFIAALKVDS"
     gene            4682..>5451
                     /locus_tag="VN968_14555"
     CDS             4682..>5451
                     /locus_tag="VN968_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL30492.1"
                     /translation="MNKPSGFGLIERACALAPLIAREADEIERTRRLTQPVVSALIEN
                     GLYRVLLPQSVGGAEAPPEIFMQMLEEVAKADASTAWCLGQCSVCAMTAAYLDPDAAR
                     EIFAAPTGILAWGSIAHEVQVVPGGYRATARWDFASGCRQASWLGAHVQIVEADGTRR
                     RKPDGAPEVRTILFPLESATLYDVWDVIGLNGTGTDSYSVDNLFIPEKFSVLRDDPAD
                     LREKGPLYRITTYTMFGLGFAAVSLGVARATLDAAIDLA"
BASE COUNT         1100 a         1722 c         1688 g          941 t
ORIGIN      
        1 gggagaatcg caaagatccc ctcttgcgcg ggggctctgg atggtcaaag tggcgcgttc
       61 gtgcggtgca ccaaaaagca cccgcgagga gtttccgatg attggcctag tacttgtgac
      121 ccatgggcgc cttgccgacg agttcaaggc ggcgctcgaa catgtcatgg gtccgcagaa
      181 acaaatcgag gccgtcacga tcggtgccga ggacgacgct gacttgtgtc gaagcgacat
      241 catcgaggcg gtcaaccgcg tcgactccgg cgacggcgtc gccatcctca ccgacatgtt
      301 cggcggaacc ccttccaatc tcgcaatctc ctgcatgagt cgcccgaagg tggaagtgct
      361 cgctggaatc aaccttccca tgctggtcaa gctcgccaag gtccgcgagg agcggtcgct
      421 ccccgacgcc atcgcgatgg cccaagaggc cggccgcaaa tacgtcacca ttgccagccg
      481 ggtgctcgcc ggcaaatgag cgacgacgct gcgtcgcccg cgcccgatgc cgggcctggt
      541 atcccgcagg gtgcgctgtc tcgcgaactt cccatcatca acaaacgcgg tttgcacgcg
      601 cgcgcttccg caaaattcgt tcagatggtc gagcgcttcg atgccgaggt ctgggtgaca
      661 cgcggcaacg agaccgtcgg cggcacctcg atcatgggat tgatgatgct gtccgcaggg
      721 cccggcacat ccatcgtcgt ctccgcggct ggcccggagg cacaagccgc gatcgacgca
      781 atcaccgaac tcgtcgccag caaattcaac gaagaatcga cgtgacccgc taacgtcatt
      841 tgcctggaag tactctatcg ccccgctctt gcgggtgtca ttccgggatg gtccgaagga
      901 ccagacctca gatgcgcaat tgcgcatcgg ggaatctcga gcttccaggt tcgcgcttcg
      961 cgcgccccgg aatgacgggg cggaccctat ttcgccggtg gcacgatata gatcttccag
     1021 ctgatcgctg gaccggggtg gccgcggaag aagcgctgcg tggtaatcgc aagctgctcg
     1081 gcgtccttgc cattctcccg catccaggct tcgcaggccg gcagcctccc gtccgtggtg
     1141 tcgcagatgc cgatgaaccc aagccgcctg gcctcctcga gcgaggtcag tcccgacgac
     1201 cagagctcgc ccggcgtgaa cggcgccgga tgatccgcac tgtagaacgt catcggctcg
     1261 ccgacctccg tagtgcctgc gacaaccgcc catcgcgtgt tgaagcgcga atgccaggcc
     1321 tgggtcagct cgcgggcgag ctgggagcgc gcgccatagg tcgcggcacc atttggatta
     1381 accgccatct cttccgtggc gatgtaaggc gaggcggcca gcgccgcgag cgtgacggtg
     1441 agccagatcg cgacaatacg aaacagcgtg atcctatgaa tgcgcagcga gggaatcgcc
     1501 accagcgcca gcggggtcag aaagaacagc gaaattcccc aatcggtctt catatagatc
     1561 gtgaaaatca agccgccgag cggtggcccg atcgcgacca cgatctggat gatccagata
     1621 ttgagcgcct gcgatctatt cacggaccga ttttcggcgc gcgaccatgc ctgcgcgaat
     1681 aaggccgacg gttgccaccc caacttcgcc cttcgtgacg gccatgccag cgccatcgcc
     1741 gccagcgcca ctggtgctgc gagcagggcg agattgtggc cgatgtagcc gagcacaagc
     1801 tgaacgcttt gcgcgcggct cgacaggctg tagacatcgc cggcgtaagt cagcggcacg
     1861 tagtccgctt ctttcagcca gaccagatgt ggaatcatcg caatgacgag cgtgacaatc
     1921 gcgacccacg gcgccggcga gcgcaggaat ttcagccggt cgggatgaat caacgcggcg
     1981 agaccgatgg cgccgatcat ggtcagcacc cagtattttg tcatcagcgc cagcgcgccg
     2041 gcgaggccga gccacagtcc cgatctcacg ccgcgctttt caaacgcgtt cagataggcc
     2101 agcacgacaa gcggcagcgt cacgagctgc aacagatccg gattgtactt gaagcccttg
     2161 aagttgaaga tcgggtagag cgcgagcatc accaccacaa agaacgcgcg gcgacgatcg
     2221 acgacgcgca aagcgatcag ccaagagatc acgagcccac agccgagcgt tgccatcgcc
     2281 agcgcgtaag ttgcccagtc ggcgaccgga aaaaccttga accagaggcc cgcgacccag
     2341 cccgccagcg gcggatgctt gccgtagccg agctggaact tctgacccca ggcgaaagct
     2401 tccgcgacat ccatatggac gtcctgcgcc gccttgagat tgatcaggat gaaggtccac
     2461 agcaccgcat gcgcgacgcc aaagccgatc accagccaca ggctcgtttt cggatcgctg
     2521 gcgcacgccg caagccacgc cgccaggcgg cggatcgacg gccggcggcg agcacgcgaa
     2581 gccgcgggca ggaacgatgc agttgacatg cgctttgcgt agcgcgtttt cgacataagg
     2641 ggaatcagct tggcgtgaag aagctccttt aaaatgctgc attaaggttg ccgcatcggg
     2701 atgcgggtgc tatctcgcgc ccatgacatc catccctatt tctaacatcc gcaacttcgc
     2761 cattgttgcg catatcgacc atggcaaatc gacgctggcc gaccgtctga tccagaccac
     2821 cggcggattg gcggcgcgcg agatgaagga acaggtgctc gattccatgg atatcgagcg
     2881 cgagcgcggc atcaccatca aggcgcagac cgtgcggctg acctatccgg ccaaggacgg
     2941 caagacctac atcctcaatc tgatcgatac tcccggccat gtcgacttcg cctatgaggt
     3001 ttcacggtcg ctggcggctt gcgaaggttc gctgctggtc gtcgacgcca gccagggcgt
     3061 cgaggcccag acgctggcaa atgtctacca cgcgctcgat gccgggcacg aaatcgtgcc
     3121 ggtgctgaac aagatcgacc tgcccgcggc ggaaccggac caggtcaaac ggcagatcga
     3181 ggatgtgatc ggcctcgatg cctccgatgc gttgatgatc tcggcaaaga ccgggctcgg
     3241 catcgacgac gtgcttgaag ccatcgtgca ccggctgccg ccacccaggg gcgaccctga
     3301 cgcctcgctg aaagcgcttc tggtcgacag ctggtacgac gtctatctcg gcgtcgtggt
     3361 gctggtgcgc attgtcgatg gcgtgatgaa aaagggccag cgcattcgca tgatgggcac
     3421 caacgcagcc tacgacgtcg agcgcgtcgg cttcttcact cccaagatgc agcagatcga
     3481 cgaacttggc ccaggcgaaa tcggctttat caccgccgcg atcaaggaag tggcggacac
     3541 cagagtcggc gacaccatca ccgacgaccg caagccggtg accgacatgc tgccgggatt
     3601 caagccggcc atccctgtgg tgttctgcgg tctgtttccg gtcgatgcca acgatttcga
     3661 aagcctgcgc gcggcgatgg gcaagctgcg gctgaacgac gccagcttct cgttcgagat
     3721 ggaaacctcg gcggcattgg gcttcggctt tcgctgcggc ttcctcggac tgttgcatct
     3781 cgaaatcata caggagcggc tgcagcgcga attcgatctg aatctcatag cgaccgctcc
     3841 gtcggtgatt tacaagatgc atctgaccga cgggcaggta atcgagattc acaatccgat
     3901 cgacatgccc gacgtggtga agatcgccga gatcgaggag ccgtggatcg aggccaccat
     3961 cctcacgccc gacgaatatc tcggtagcgt actgaaattg tgccaggacc ggcgcggcgc
     4021 gcagaaggag ctcacttacg tcggcaatcg cgccatggtg aaatacgagc tgccgctcaa
     4081 cgaggtggtg tttgatttct acgaccggct gaaatcggtc tcaaagggct atgcgtcgtt
     4141 cgactaccat ctcaccgact acaagcccgc cgatcttgtg aagatgcaga ttctcgtcaa
     4201 caacgagccg gtggatgcgc tgtcgatgct cgtgcaccgc cagcgcgcgg aagggcgcgg
     4261 ccgcgccatg gtcgagaaga tgaaggaact gatcccgccg cacatgttcc agatcccgat
     4321 ccaggcggcg atcggcggca aggtgatcgc ccgcgagacc gtgcgcgcgc tgcgcaagga
     4381 cgtcaccgcg aagtgctacg gcggcgacat cacgcgcaaa agaaaacttc tggagaagca
     4441 gaaggaaggc aagaagaaga tgcggcagtt cggcaaggtc gacatcccgc aggaagcctt
     4501 catcgccgcg ctgaaggtgg atagttgagg cggatcgcac tgcttctggt cctcggcttg
     4561 gcccgtcgag cgcacacccc gcgcctctcg cattcctgtc cccatcttga tcggccgccg
     4621 cgaatagacc accattgcgg cttcgccggc caaagaaccc caaagagggc gaggaaacgc
     4681 aatgaacaag ccttccggtt tcggactgat cgagcgcgcg tgcgcgctcg cgccgctgat
     4741 cgcgcgggag gccgatgaaa ttgagcggac acggcgtctc acgcagccgg tggtgtcagc
     4801 cctgatcgag aacgggcttt atcgggtgct gctgccacaa agcgtcggtg gcgccgaggc
     4861 gccgccggaa atcttcatgc agatgctgga ggaagtggcg aaggccgacg cctccaccgc
     4921 gtggtgcctc ggccaatgca gcgtctgcgc catgacggcg gcctatctcg atcccgacgc
     4981 ggcgcgcgag atttttgccg ccccgaccgg catccttgcc tggggctcga ttgcccatga
     5041 agtgcaggtc gtgcccggcg gttatcgcgc caccgcacgc tgggacttcg ccagcggctg
     5101 ccgtcaggcg agctggctcg gcgcgcatgt ccagatcgtg gaggccgatg gcaccaggcg
     5161 ccgcaagccc gacggcgcgc cagaggtgcg caccatcctg tttccgctgg aaagcgccac
     5221 gctatacgat gtctgggacg tgatcggcct caacggcacc ggcacggatt cctactcggt
     5281 cgacaacctg ttcatcccgg aaaaattttc ggtgctgcgg gacgatcccg cggatctgcg
     5341 tgagaagggc ccgctctatc ggatcacgac ctacaccatg ttcggactgg gctttgccgc
     5401 ggtctcgctc ggcgtcgcgc gcgccacgct cgacgcggcc attgatctgg c
//
LOCUS       DATNOI010000364         7862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1682694,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000364 DATNOI010000000
VERSION     DATNOI010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682694"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VN968_14560"
     CDS             <1..453
                     /locus_tag="VN968_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL30493.1"
                     /translation="HICEVEIDPETGVSKVVRYTTVDDVGVAVNPMLATGQVHGGVAQ
                     GIGQALYEGVSYDPEGQLLTASYQDYCVPRADDIPPIEVTLDDSAPCRTNPLGAKGCG
                     ESGAIGGPPCITNGVMDALSEFGIKNLNTPLSPLKVWQAIQEAKAARV"
     gene            700..1311
                     /locus_tag="VN968_14565"
     CDS             700..1311
                     /locus_tag="VN968_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXL30494.1"
                     /translation="MRRFWTVLAALATLSLTNCGYNAIQSQDEQVKSSWSEVVNQYQR
                     RADLVPNLVNSVKGFAQQEKDVLLGVTNARAKVGSIQATPEVLNDPAAFQKFQAAQGE
                     LSSALSRLLVVTENYPQLKSDALFHDLMAQLEGTENRITVARNRYIKTVQDYNVTIRS
                     FPTNLTAMGFGYKEKPNFSVENEKEISTAPKVDFNPAPAAPAK"
     gene            1334..2221
                     /locus_tag="VN968_14570"
     CDS             1334..2221
                     /locus_tag="VN968_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HXL30495.1"
                     /translation="MNTARASFLALLLCWAFAAFADVAVPPLVGRVVDQTGTLSSDEI
                     ASLNQTLRNFEARKGSQVAVLIVPSTAPEAIEQYSLRVAETWKIGRKKIDDGALLVVA
                     KEDRKLRIEVGYGLEGALTDVTSKRIIDEIITPRFRSGDFAGGISAGVDRIIRVVDGE
                     SLPAPAPRQQQSSDFLSHVDLLNPFTLFAVFIVSTILRTTLGRLIGAVATGGIVGALA
                     WLIAGSLAASFIAAIIAFLFTMFAEIPTSSSGRGSGRGWSSGGSYSGGSWGGGSSSSS
                     SDSGGGGGSFGGGGASGSW"
     gene            2238..2735
                     /locus_tag="VN968_14575"
     CDS             2238..2735
                     /locus_tag="VN968_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXL30496.1"
                     /translation="MGIGRIGKHLLEHRWRVRRIFPPRVLAAIEQAIKAGEATHSGQI
                     RFVVEGALDGAPLFRDQPARERALDIFSQLRIWDTAHNNGVLIYLLLADRDVEIVADR
                     GIDAKVGAAGWEKICTDMETEFEAGNFERGVINGIEAVSKQLAAHYPRQGAGRNELPD
                     APVVI"
     gene            complement(2910..3722)
                     /locus_tag="VN968_14580"
     CDS             complement(2910..3722)
                     /locus_tag="VN968_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL30497.1"
                     /translation="MSDRKPSPFPVLLNNDVCSLIQAAEEVYQIRFKNRAANAYLVRG
                     KSRTIMIDVGLSSNYPALLECLDHVGCSPDKIDILVLSHEHLDHIGAAYHFGGRTIIA
                     AHRLAANKIMLRDDFAMLRKMFNEPNVPINVDIWLEEGNLIDLGNFRLNVMYTPGHTS
                     ACISLFDQDQGLLFAADTLMPGGVMGGVFGSGSISDYIQSLERLKGLNSRILLSGHGR
                     LSDTPQDDVRIAIQRSHMLLSDTAQLFDALDARSNFEPIMQSVRDLNKLDDG"
     gene            complement(3719..4090)
                     /locus_tag="VN968_14585"
     CDS             complement(3719..4090)
                     /locus_tag="VN968_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXL30498.1"
                     /translation="MAIEKCITEYGVDDVIFEEGSTGRELFVVLDGIIDIVRINGTSK
                     TLIVTLGKGEFFGEMAVIDGSSRSATAIASAPNTRVMRINHARFVYLVSQQPAFALMI
                     MDALSKRLRASNDVIFGAANP"
     gene            4341..5324
                     /locus_tag="VN968_14590"
     CDS             4341..5324
                     /locus_tag="VN968_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30499.1"
                     /translation="MSEQMADQSSDESGSLKAALGSLETPSTPFAVSANESVPETTNV
                     DSPKLSPEHGGPGETRKVDPAKAEAPKADAPKADAPKPDAPKPDARRMPGSITIMSAG
                     DRAGSAPKAEQGSGLLGKRGIAALAAVAALAAIAGALGGALATAGVGHFLGDGATASA
                     SPTLEASVARIDADILTLMANLENISKTGTAQFNKTNDRLDKVEKAQAEPAAKLAKLS
                     EAVDKLRGTPVAASAAAATPAAAKDVTGSISAPATTPAAAPKPEVARMPTVEGWVLRD
                     VTHGAALIEGRRGMYEVYAGDPIPGLGRVDAIRRQDGRWVVVTGKGLIVAR"
     gene            5507..7054
                     /locus_tag="VN968_14595"
     CDS             5507..7054
                     /locus_tag="VN968_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30500.1"
                     /translation="MRILVTTLVLLCGVLTARAGQSALERGTAIIDPQGLRELDRGRL
                     GLSGVILPMRSSNAPLTSSELFALPEMVPVRKALDDEFDRYIAHHKASLPNETIGVGT
                     AFDFQLFDRTQLYSRDSRFVLAGIVNRMDRAYLSAANCGEIRLIYRLVRTDAPAIGEN
                     AVSPRLPMTLNIVLKAKADNAIDANGAAITCSEIARRWLAAGDSELTGTELAEKLASK
                     DGPLALIRPENIDRIETNLQIAHAPKSAVRDFRTDYLMKVFDYKSQSGTFEEAPLENQ
                     IDRERILGDQALKRDFKAWLLDSTHFSELDRGTILIPEKFLARRAIAPTPVGFGASDL
                     QPAFGLVEGDDATPDPVFKQADVVAALKQAADAGVTLQNIRSAAGLERRLNDITCGGC
                     HQTRGIGGFHFPGVDWMAAKPSNSTVVPASPHFFGDQIRRRDILTSLRNGTPPDYSRG
                     FSSRPQLRGSTELAGTSFEDGWGALCYQHKLEAADNDASFQSWRCAEELTCQPVDKAS
                     RIGMCFR"
     gene            7249..>7862
                     /locus_tag="VN968_14600"
     CDS             7249..>7862
                     /locus_tag="VN968_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018856979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXL30501.1"
                     /translation="MDDIIIVGAGVGGLTLGLALHAAGIPCRIFESAAEIRAIGVGIN
                     LLPHATRELAALGLEAELARVAIATEDATFFNRFGQLIYSEPLGRAAGYEHPQFSIHR
                     GDLQMVLLEAFRARAGRDRLNTNHHCTGVEQNETGVSVSFSDGPEGTGRSTVRGRAAI
                     ACDGINSAVRKQFYPGEGEPRYSGVNMWRGVTRWKPMLSGASMVR"
BASE COUNT         1490 a         2417 c         2508 g         1447 t
ORIGIN      
        1 catatctgcg aagtcgagat cgatccggag accggcgtca gcaaggtcgt tcgctacacc
       61 acggtcgacg acgtcggcgt cgccgtcaat ccgatgctgg cgacggggca agttcacggc
      121 ggcgtcgcgc agggcatcgg acaggcgctg tacgagggcg tctcctacga ccccgaaggg
      181 cagttgctga ccgcgagcta tcaggattac tgcgtgccgc gcgccgacga cattcctccg
      241 atcgaggtga cgctcgacga ctccgcaccc tgccgcacca atccgctggg cgccaagggt
      301 tgcggcgaat ccggcgcgat cggcgggccg ccctgcatca ccaatggcgt aatggacgcg
      361 ctcagcgagt tcggcatcaa gaatctcaac accccgctgt cgccgctgaa agtctggcag
      421 gcgatccagg aggccaaggc ggcgcgggtt tgactcattt ccttagcctc gtcattccgg
      481 gatgcgccac cgaactcggg tttacccgag ttcggcaata tcattgtcca agtcggcaat
      541 tgccgacctg aggcgcaggc ccggaatcca tactccctgc aggggtgatg gattccaggc
      601 tcgctcgctt cgctcgcgcc ccggaatgac ggggaaaaaa tttacagccc ttggcatcgt
      661 tctgatacct tgcgaaacaa tataacggga gcgattgcga tgcgcagatt ttggaccgtg
      721 ctggcggcgc tggcgacctt gagcctcacc aattgcggct acaacgcgat tcagtctcag
      781 gacgaacagg tcaagtcaag ctggtcggag gtcgtcaacc agtaccagcg ccgcgccgat
      841 ctggtaccca acctcgtcaa ttcggtgaag ggttttgcgc agcaggaaaa ggatgtgctg
      901 ctgggagtga ccaatgcccg cgccaaggtc ggcagcatcc aggccacgcc ggaggtcctc
      961 aacgatcccg cggcgttcca gaaattccag gcggcgcagg gcgaactctc aagcgcgctg
     1021 tcgagattgc tggtcgtcac cgagaattat ccgcagctca aatccgacgc gctgtttcac
     1081 gacctgatgg cgcaactgga gggcaccgag aaccgcatca cggtggcgcg caaccgctac
     1141 atcaagaccg tgcaggacta caacgtcacg attcgctcgt ttccgaccaa cctgacggcg
     1201 atggggttcg ggtacaagga gaagccgaat ttctcggtcg agaacgaaaa ggaaatctcg
     1261 actgcgccga aggtggattt caatccggcg ccggcagcgc ccgcaaagta gttcgacaac
     1321 cagattgatc gcgatgaaca ctgcaagagc atcctttctt gcgttgctgc tgtgctgggc
     1381 ttttgcggcc tttgccgatg tcgcggtgcc acctctggtc gggcgggttg tcgaccagac
     1441 cgggacgctt tccagcgacg aaatcgcctc gctgaaccaa acgctgagga atttcgaggc
     1501 gaggaaaggc agtcaggtcg cggtgctgat tgtgccgagc accgcgcccg aggccatcga
     1561 gcaatattcg ttgcgggtcg cggaaacctg gaagatcggc cgcaagaaga tcgacgacgg
     1621 cgcgctgctt gtcgtcgcca aggaagaccg caagcttcgc atcgaggtcg gctacggcct
     1681 cgaaggcgcg ctgaccgacg tcacctcgaa acgcatcatc gacgagatca ttacgccgag
     1741 attcaggagc ggcgattttg ccggcgggat ttcagccggt gtcgaccgga tcatccgggt
     1801 ggtcgacggc gagagtttgc ccgcgccggc gccgcgtcag cagcaaagct ccgacttcct
     1861 gagccatgtc gatctgctca atccgttcac gttgttcgcg gtctttatcg tcagcacgat
     1921 cctgcggacc acgctgggca ggctgatcgg cgcggttgct accggcggca tcgtcggagc
     1981 gttggcatgg ctgatcgcgg gatcgctggc ggcgtctttc atcgcggcga tcattgcctt
     2041 cctcttcacc atgtttgcgg agatccctac ttcttcgtcc ggtcgcggca gtgggagagg
     2101 ctggtccagc ggcggttcgt attccggtgg ttcctggggc ggcggtagca gcagtagttc
     2161 gagcgacagc ggcggggggg gcggcagttt cggcggcggc ggcgcgtccg gaagctggtg
     2221 atcgggagcg ggtagatatg ggcatcgggc gcatcggcaa gcaccttctc gagcaccgct
     2281 ggcgggtgcg gcggattttt ccgccaaggg tgctggcggc aatcgaacag gcgatcaagg
     2341 ccggcgaggc gacgcattcc ggccagatcc gttttgtcgt ggaaggcgcg ctggacggtg
     2401 cgccgctatt tcgggatcag ccggcgcggg agcgtgcgct ggatattttt tcgcaattgc
     2461 ggatctggga caccgcccac aataacggtg tgctgatcta tctgctgctg gccgatcgcg
     2521 atgtcgagat tgtcgccgat cgcggcatcg acgcgaaggt tggcgctgcc ggctgggaga
     2581 aaatctgcac cgacatggag accgaattcg aagcggggaa tttcgagcgc ggtgtgatta
     2641 acggaatcga ggcggtgtcg aaacaactgg cggcgcatta tcccaggcag ggcgcggggc
     2701 ggaacgaact gccggatgcg cctgtggtga tttgagactt gtcattccgg ggcgtgagcc
     2761 aacgggtccg gcctctggcc ggcccgatga caggctccgc gaacgagccc ggaatccata
     2821 accacgaccg ggagtatgga ttccgggcct gcgcctcagg tcggcaattg ccgaccgaag
     2881 gcgcatcccg gaatgacgag tggttgatct cagccgtcgt cgagcttgtt gaggtcgcgc
     2941 accgactgca tgatcggctc gaaattcgat cgcgcgtcga gcgcgtcgaa caactgggcg
     3001 gtgtccgaca acagcatgtg cgagcgctgg atcgcgatcc ggacatcgtc ctgtggcgtg
     3061 tcggatagcc ggccatgtcc cgacaacagg atcctggagt tcaagccctt cagccgctcc
     3121 agcgactgga tgtagtcact gatgctgccc gagccgaaca cgccgcccat caccccgccc
     3181 ggcatcaggg tgtcggcggc aaacagcagt ccctggtcct gatcgaacag gctgatgcag
     3241 gccgaggtat ggcctggcgt gtacatcacg ttgagacgga agttgccaag gtcgatcagg
     3301 tttccttctt cgagccagat atcgacattg atcggcacgt tgggctcgtt gaacatcttg
     3361 cgcagcatcg cgaaatcgtc gcgcagcatg atcttgttgg cggcgagccg atgcgcggca
     3421 atgatggtcc gtccgccgaa atgatacgcc gcgccgatgt gatcgagatg ctcgtggctc
     3481 agaaccaata tgtcgatctt gtccggcgag cagccgacat gatcgaggca ttccagcaga
     3541 gccgggtaat tcgaggatag cccaacgtcg atcatgatgg ttcgggattt tccgcgcacc
     3601 agataggcgt tcgcggcgcg gttcttgaag cggatctgat agacctcctc ggccgcctgg
     3661 atcagcgagc agacgtcatt gttcagaaga accgggaacg ggctcggctt gcggtcgctc
     3721 atgggttggc tgctccgaag atgacgtcgt tggacgcgcg cagtcgcttg ctgagcgcat
     3781 ccatgatcat cagcgcgaac gccggctgct gactgaccag atagacgaac cgggcatgat
     3841 tgatccgcat cacgcgggtg ttgggtgctg aggcgatcgc ggtggccgag cgggacgagc
     3901 cgtcgatcac cgccatctcg ccgaagaatt cacccttgcc cagggtcacg atcaacgtct
     3961 tgctggtgcc gttgatcctg acgatgtcga ttataccatc gagcaccacg aatagttcgc
     4021 gcccggtcga gccttcctcg aaaatgacat cgtccacgcc atattcggtg atgcacttct
     4081 cgatggccat ggagccccct gccttctggc tggtccgcgg cgccatgtta atcgggaaac
     4141 agggccgcgc aaacgccgga tcggatcccc cgccatttct gcagggcagc atgggccgag
     4201 tagaagcgct gcggccaccc tgacgggatc gaaaagcggg ccgggtaatt ccctaaaatt
     4261 tcggtaaatc gggccccggt aacgatttgg caagaaatta aaggttaccg aatggtcaac
     4321 gtttactgga ggttttgaac atgagcgagc aaatggccga tcagagcagc gatgaatccg
     4381 gttccctcaa ggccgctctc ggttccctgg agacgccgag cacgccgttc gcggtgtcgg
     4441 cgaacgaatc ggtgccggaa accaccaatg tcgattctcc gaagctgtcg cccgagcacg
     4501 gcggacccgg cgaaacccgg aaggtggacc cggcaaaggc cgaagctccc aaggctgatg
     4561 ctcccaaggc tgatgctccc aagccagacg ccccgaagcc cgacgcacgg cgcatgccgg
     4621 gctcaatcac gattatgtca gccggcgacc gcgccggttc cgcaccgaag gccgagcagg
     4681 gctccgggtt gctcggcaag cgcgggatcg cagcactggc ggccgtggcg gcgctggcgg
     4741 cgatcgcagg cgcgctcggc ggcgcactgg cgaccgcggg tgtcgggcat tttcttggcg
     4801 atggtgccac ggcgtcagcc agtcccacgc tcgaagcgtc ggtggcgcga atcgatgccg
     4861 acattctgac gctgatggcc aaccttgaaa atatctccaa aaccggcacg gcccagttca
     4921 acaagaccaa cgatcgcctc gacaaggtcg agaaggcgca ggccgaaccg gctgccaagc
     4981 tcgccaagct cagcgaggcc gtcgataagc tccgcggtac gccggttgct gcgtccgctg
     5041 ccgctgcaac gcccgccgcg gcaaaggacg tcactggctc gatttcagcg ccggcgacaa
     5101 cgcctgccgc cgcgcccaaa cccgaggtgg cgagaatgcc gacggtagag ggctgggtgc
     5161 tgcgtgacgt cacccatggc gctgccctga tcgaaggccg ccgggggatg tacgaagtgt
     5221 atgccggcga tccgataccg ggcctcggcc gggtcgacgc catccggcgt caggacggcc
     5281 gctgggtggt cgtcaccggc aaaggcctga ttgtcgcgcg ctagcgcatg atcgcttcgc
     5341 ttccccgatc gcttcaccac gacgtgaagc gatggccgga cccggtccgg atctgcgcga
     5401 caggccgtct gcttttcggc taatttcctg actaggaaga cgttgcccgc aagagccttg
     5461 agcaggcgct cttgatgcgc ggcgttcgtc gggatatggg ccgcgcatgc gaatccttgt
     5521 cacaaccctt gtcttgctgt gcggtgtctt gacggcgcgc gccgggcaga gcgcgcttga
     5581 gcgcggcacc gcgatcatcg atccccaggg cttgcgtgaa ctcgatcgcg gaaggcttgg
     5641 cctgagcggc gtgatcctgc caatgcgatc gtccaacgca ccgctcacca gcagcgagct
     5701 atttgcgttg ccggagatgg tgccggtgcg caaggcgctc gacgacgagt tcgatcgtta
     5761 catcgcccat cacaaggcca gtcttccgaa cgagaccatc ggcgttggca cggctttcga
     5821 ttttcagctg ttcgatcgca cacagcttta ctcccgcgat tcacgcttcg tgctggcggg
     5881 tatcgtcaac cggatggacc gcgcctatct ttcggccgcg aattgcggtg agatccggct
     5941 gatctatcgt ctggtgcgga cggacgcgcc ggcgatcggc gaaaacgcag tgtcgccgcg
     6001 gctgccgatg acgctcaaca tcgtgctgaa ggccaaagcc gacaacgcga tcgacgcaaa
     6061 cggtgcggcg atcacctgtt cggagatcgc gcggcggtgg cttgccgcag gcgattccga
     6121 gctcaccggc acggagcttg cggaaaaact ggcgtcgaag gatggcccgc tggctctgat
     6181 ccggcctgaa aacatcgacc gcattgaaac caatcttcag atcgcgcacg cgccgaaatc
     6241 cgcggtccgt gattttcgca ccgactacct catgaaggtg tttgattaca aatcccagtc
     6301 cggcactttc gaggaagcgc cgctcgagaa ccagatcgat cgtgagcgga ttcttggcga
     6361 tcaggcgctc aagcgcgatt tcaaggcgtg gctgcttgat tccacccatt tcagcgaact
     6421 ggatcgcggc acgattttga tccccgaaaa attcctcgcc cgccgcgcga tcgcgccgac
     6481 gccggtcgga tttggggcct cggacctgca accggccttt ggcttggtgg agggcgacga
     6541 tgcaacgcct gaccccgtgt tcaagcaggc tgacgtggtc gcggccttga agcaggcggc
     6601 ggacgccggc gtgaccttgc aaaacatccg ttcggctgcc ggccttgagc gccggctcaa
     6661 cgacatcacc tgcggcggct gccatcagac ccgcggcatc ggcggctttc atttccccgg
     6721 cgtcgactgg atggcggcaa aaccctccaa ctcaacggtg gtgccggctt cgccgcattt
     6781 cttcggcgac cagatccgcc gtcgcgatat tctgacctcg ctgcgcaacg gcacgccgcc
     6841 ggattactcg cgaggctttt ccagccgtcc gcaattgcgc ggcagcaccg agcttgccgg
     6901 gacctcattc gaagacggct ggggtgcgct gtgttaccag cacaagctag aggccgccga
     6961 caatgacgcg agcttccagt catggcgctg cgccgaggaa ctcacctgcc agcccgtcga
     7021 caaggcttcc cgaatcggca tgtgttttcg ttaaaagacc gctaaccgcg aggcctcgtg
     7081 tcccggacgc gttcttcacg ctcctccgca gagccgggac cgacacagat gcggtacgca
     7141 agataacccc ggattgcacg catttccctg gattgacagt aggctcacca atcgtgtttc
     7201 atcgccgcaa tcgcctgaca gagcgagcag acattgccgg gagcagacat ggacgacatc
     7261 attatcgtcg gggccggcgt cggcggattg accctggggc tggcgctgca cgccgcagga
     7321 atcccctgcc ggatatttga gtcggcggcc gaaatcaggg cgatcggggt cggcatcaac
     7381 ctgcttccgc atgccaccag ggaattggcc gcgctcggac tcgaagcgga actggcgcgg
     7441 gtcgcgattg ccaccgagga cgcgaccttc ttcaatcgct tcggccagtt gatctattcc
     7501 gaaccgctcg gccgggccgc gggttacgag catccgcaat tctccatcca tcgcggcgac
     7561 ctgcagatgg tcctgctcga ggcttttcgc gcgcgtgccg gacgcgatcg gctcaacacc
     7621 aaccatcatt gcaccggcgt cgaacagaat gagaccggcg tcagcgtcag cttctccgac
     7681 gggcctgaag gcaccggccg cagcaccgtg cgcggccgcg ccgcgatcgc ctgcgacggc
     7741 atcaattccg ccgtccgtaa gcagttttac cccggcgaag gcgagccgcg ctattccggc
     7801 gtcaacatgt ggcgcggcgt aacgcgatgg aaaccgatgc tgtccggcgc cagcatggtg
     7861 cg
//
LOCUS       DATNOI010000365         6167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_480714,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000365 DATNOI010000000
VERSION     DATNOI010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480714"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VN968_14605"
     CDS             complement(<1..674)
                     /locus_tag="VN968_14605"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXL30502.1"
                     /translation="MADGLRKGLTSYGDAGFSLFLRKAFIKAMGYSDDALNRPIVGIT
                     NTHSDYNPCHGNVPEIIEAVKRGVMLAGAMPMVFPTISIAESFAFPTSMYLRNLMAMD
                     TEEMIRAQPMDSVVVIGGCDKTLPAQIMAAVSADLPTVVIPVGPMVVGHHKGEVLGAC
                     TDCRRLWAKFRAGEIDEAEIEAVNGRLAPSVGTCMVMGTASTMACITESLGLSLPMSA
                     TIPAPHA"
     gene            complement(818..1636)
                     /locus_tag="VN968_14610"
     CDS             complement(818..1636)
                     /locus_tag="VN968_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL30503.1"
                     /translation="MPRILMTGASGGIGTSLRKLLPPIYPDLLLSDLKAPADLGAHEK
                     FRAAELSDPAQVEAICDGVDGILHFGGYSVEGPWDDILQSNIIGGYNLFEAARKKGVK
                     RVVFASSNHAVGFYPRHHHIGTDVTPRPDSRYGVSKVFGEAVGALYADKYGLGVTCIR
                     IGNFGNKPLDHRRLSIWLKPEDLVQLCRIGLDHPDIHFEIFYGASYNERAWWDNHRAY
                     DLGYRPTGRAEDFREHAFAEQAKLPPDPVGDFYQGGAFCGMEFDGDMSRIIDLK"
     gene            1927..2895
                     /locus_tag="VN968_14615"
     CDS             1927..2895
                     /locus_tag="VN968_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXL30504.1"
                     /translation="MSETVPHSPGWRPATYYPDPAIHALDPRFEKYWLKLSAVERLTT
                     GLRWAEGPVWFGDGRYLLCSDIPNNRIIKWEEETGSVSIFRKPSNFANGHTRDRQGRL
                     ITCEHGGRRVLRTEYDGSITVLMDSFDGKRLNSPNDVVVKSDGSIWFTDPVFGLLGNY
                     EGYKAEPELDMNVYRIDGATGKASIVAEGVLGPNGLCFSPDEKILYVIESRGVPTRKI
                     LAYDVTASGDKVSNKRVFVDAGPGTPDGMRADIDGNLWCGWGMGDPELDGVVVYAPDG
                     ALIGRIALPERCANLCFGGLKRNRLFMAASQSIYALYVNTQGALGG"
     gene            complement(3115..4545)
                     /locus_tag="VN968_14620"
     CDS             complement(3115..4545)
                     /locus_tag="VN968_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXL30505.1"
                     /translation="MVNRMQFYIDGAWVDPAVKKSTPVVNPATEEAMYEVALGSKADV
                     DKAVAAARRAFETFSRTSREERVALLSKIIEVYRTRMKDIGAAVSDEMGAPLPMAEKL
                     QAGAGLGHLASTLDVLKNYHFEEPVGSAMVVREPVGVIGMITPWNWPLNQIACKVAPA
                     LAAGCTMILKPSEFTPSSALIFAEILHEAGVPKGVFNLINGLGPEVGAAMSEHPDIDM
                     ISFTGSTRAGVDVAKRAAPTVKRVSQELGGKSPNVILEGADLAKAVTGGVMHMFNNSG
                     QSCNAPSRMIVPLSKMKEVAAIAKGVADNTKAGDPRGEGTTIGPVVSRIQWDKIQTLI
                     QKGIEEGATLVAGGPGLPDGVNKGFYVRPTIFADVTNDMTIAREEIFGPVLTILGAKD
                     EEDAVKIANDTPYGLAGYVTADSVETARRVGRRIRAGNVNLQGVPNERTAPFGGYKQS
                     GNGREWGKYGLEEYLEVKAVAGYNAA"
     gene            complement(4846..>6167)
                     /locus_tag="VN968_14625"
     CDS             complement(4846..>6167)
                     /locus_tag="VN968_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXL30506.1"
                     /translation="AQNIRPGHEWEKFEGLSQDPAALEAFLGQAPFNRDGRRRLTADV
                     IEVPEQLSFKPSRQMSTQQGFGLVLNELARGDSELASRIVTASPDVTVSTNLGAWVNR
                     RGLFARAEKADLFRSEKIPSTFNWDFSPKGQHIELGIAEMNLFILMAALGLSHAINGE
                     RLLPIATLYDPFIQRGLDALNYACYQDARFMVAATPSGITLSPEGGAHQSIATPLIGI
                     AQDGLASFEPAFVDELAAIMGWGFQHMQREGAGEGGSVYLRLSTRTLEQPQRIMTPDL
                     QKAITDGAYWLRKPGPNAEVVIAYTGAVAPEAIESIGLLGESRRDVGLLAITSADRLH
                     AGWTAARKLRRDRRGMQYLSHVEQLLAPLPRDCGIVTVIDGHPATLGWLGSVRGHRVE
                     ALGVEHFGQTGTIDDLYRHHGMDANAIIDAAEALTAGAPVRHRKMAV"
BASE COUNT         1130 a         1952 c         1963 g         1122 t
ORIGIN      
        1 gcatgcggcg ccggaatcgt cgcactcatc ggcagcgaca ggcccagcga ctcggtgatg
       61 caggccatgg tagaggcggt gcccatcacc atgcaggtgc cgaccgaggg cgccagccgg
      121 ccattgaccg cctcgatctc cgcctcgtcg atttcgccgg cgcggaattt cgcccacaga
      181 cggcggcaat cggtgcaggc ccccagcact tcgcccttgt gatgcccgac caccatcggc
      241 cccaccggaa tcacgacggt cggcagatcg gctgagaccg cggccatgat ctgcgccggc
      301 aaggtcttgt cgcaaccgcc gatcacgacg accgaatcca tcggctgggc gcggatcatc
      361 tcctcggtat ccatcgccat cagattgcgc agatacatcg aggtcgggaa cgcaaaactt
      421 tcggctatcg agatggtcgg aaacaccatc ggcatggcgc cggccagcat cacgccgcgc
      481 ttcaccgcct cgatgatctc cggcacgttg ccgtggcagg gattgtagtc actgtgggtg
      541 ttggtgatac cgacgatcgg gcgattgagc gcatcatcgg aatagcccat ggccttgatg
      601 aaggccttgc gcaggaacaa cgaaaatccg gcgtcgccat aactcgtcag tcccttgcga
      661 agtccatcag ccatctgaat ttcctctttg ccccacctta cgtcggggca cgcgtaactt
      721 gaaggcgtca ttccggggcg ggagcgaagc gagcgaaccc ggaatctcga gattccgggt
      781 tcgctctggc gcgcgccccg gaatgacatc aaaggcgcta cttcaagtcg ataatcctgc
      841 tcatgtcgcc gtcgaactcc atgccgcaaa atgcgccgcc ctggtagaaa tcgccgaccg
      901 gatccggcgg cagtttcgcc tgctcggcaa aagcatgttc gcgaaaatcc tcggcccggc
      961 cggtcgggcg gtaaccgagg tcgtaggcgc ggtggttgtc ccaccaggcg cgctcgttgt
     1021 acgacgcgcc gtagaaaatt tcgaaatgga tgtcgggatg atcgagcccg atccggcaga
     1081 gctgcaccag gtcctcgggc ttgagccaga tcgacagccg gcgatgatcc agcggcttgt
     1141 tgccgaaatt gccaatccgg atgcaggtga cgccgagccc gtacttgtcg gcataaagcg
     1201 cccccaccgc ctcgccaaac accttgctga cgccgtagcg gctgtcgggg cgcggggtta
     1261 catcggttcc gatgtggtga tggcgcggat aaaagcccac cgcatggttc gatgacgcga
     1321 acacgacgcg cttgacgccc tttttccgcg cggcctcgaa caggttgtag ccgccgatga
     1381 tattggactg caggatgtca tcccaggggc cttcgaccga gtagccgccg aaatgcagga
     1441 tgccgtcgac gccgtcgcag atcgcctcga cctgcgcggg atcggatagc tcggctgccc
     1501 tgaatttttc gtgagcgccg agatcggcgg gcgccttcag gtcgctcagc agcaaatccg
     1561 gatagatcgg cggcagcaat ttgcgcaaac tcgtgcctat tccccccgat gcgccggtca
     1621 tcaatatgcg tggcatttca tcccctcgtt tgtggcttga agcgagtatg gcgcgacggc
     1681 ggcagcgcgg caagcgatgg cgcgcgacac aaatatagcc gtcatacccc gcgaaagcgg
     1741 ggtatccagt aatcgccaac gtcaatgttt actggatcat ccgatggagc ctgtcatcgg
     1801 gcgagcgctg cgccgacccg tggcggatga tgacatccgt aagagccgac ccgcttgctg
     1861 ttacagaaag gcaatgatag cagaccaaga cttgtgagga attcaagaca gcaacgagaa
     1921 cagcaaatgt ccgaaaccgt accccattcc cccggctggc ggccggcgac gtattatccc
     1981 gatccggcca tacacgcgct cgatccacgg ttcgagaaat actggctcaa gctctcggcg
     2041 gtggaacggc tgacgacggg attgcgctgg gccgagggac cggtctggtt cggcgacggg
     2101 cgctatttgt tgtgcagcga tattcccaac aaccgcatca tcaagtggga agaggaaaca
     2161 ggctccgtca gcatttttcg caaaccgtcg aatttcgcca acggccatac ccgcgaccgc
     2221 cagggccgcc tcatcacctg cgagcatggc ggccgccgcg tcctcagaac cgaatatgac
     2281 ggctcgatca cggtactgat ggactcgttc gacggcaagc ggctgaactc gccgaacgac
     2341 gttgtggtca aatccgacgg ctcgatctgg tttaccgatc cggtgttcgg cctgctcggc
     2401 aattacgaag gctacaaggc cgagccggag ctcgacatga acgtctatcg gatcgatggt
     2461 gcgaccggaa aggccagcat tgtcgccgaa ggcgtgctcg gtccgaacgg gctgtgcttt
     2521 tcgccggacg aaaaaattct ctacgtcatc gaatcgcgcg gggtgccgac ccgcaagatc
     2581 cttgcctatg acgtcacagc cagcggcgac aaggtttcca acaagcgcgt gttcgtcgat
     2641 gccggccccg gcacgcccga cggcatgcgg gccgacatcg acggcaatct gtggtgcggc
     2701 tggggcatgg gcgatccgga actcgacggc gtggtggtct acgcgcccga cggcgcgttg
     2761 atcggccgca tcgcgctgcc cgagcgctgc gccaatctgt gtttcggcgg gttgaagcgc
     2821 aaccggctgt tcatggccgc cagccagtcg atctacgcgc tgtacgtgaa cacgcagggc
     2881 gcgctgggag gataaggcac ggcacatctc cacgggtcgt ccccgcgcag gcggggaccc
     2941 atacgccgag gattctcgtt ttaggcattg gagcaaatgc ccttctgcta aaatggacgt
     3001 taggggttat gggtcccggc gttcgccggg acgacgctga gttcttgttc gccgctatca
     3061 acgcaaaacg ccggagcgga attacccgcc ccggcgctgc aattttcgca agccttacgc
     3121 cgcgttgtag ccggcgaccg ccttgacctc gagatattcc tccaggccgt atttgcccca
     3181 ttcgcggccg ttgccggact gcttgtagcc accgaacggc gcggtgcgct cattcggcac
     3241 gccctgcagg ttgacgttgc cggcgcggat ccggcggccg acccggcgcg cggtttccac
     3301 gctgtcggcg gtgacgtaac cggcgagacc gtaaggcgtg tcgttggcga tcttcaccgc
     3361 atcctcttcg tccttggcgc ccaggatggt gagcacgggg ccaaaaatct cttcgcgcgc
     3421 gatggtcatg tcgttggtga cgtcggcgaa gatggtgggg cgcacgtaaa atcccttgtt
     3481 gacaccgtcc ggcagaccgg gaccgccagc aaccagggtg gcgccttcct cgatgccctt
     3541 ctggatcagg gtctggatct tgtcccactg gatgcgggac accacgggac cgatggtggt
     3601 gccttcgccg cggggatcgc cggccttggt gttgtcggcg acgcccttgg cgatggcggc
     3661 gacttccttc atcttcgaca gcggcacgat catccgcgac ggcgcgttgc aggactggcc
     3721 ggagttgttg aacatgtgca tcacgccacc ggtgacggcc ttggcgagat cggcgccttc
     3781 caggatgacg ttcggcgact tgccgccgag ttcctgactg acacgcttga cggtcggggc
     3841 tgcgcgcttg gcgacgtcga cgccggcgcg ggtcgagccg gtgaacgaga tcatgtcgat
     3901 gtccgggtgc tcgctcatgg cggcgccgac ttcagggccg aggccgttga tcaggttgaa
     3961 gacgcccttc ggcacgccgg cctcatgcag gatttccgcg aaaatcagcg ccgatgacgg
     4021 cgtgaattcg ctcggcttca ggatcatggt gcagccggcg gcgagcgcgg gcgcgacctt
     4081 gcaggcgatc tggttcaagg gccagttcca cggcgtgatc atgccgatga cgccgacggg
     4141 ttcgcgcacc accatcgccg agccgaccgg ctcttcgaaa tgatagtttt tcagaacatc
     4201 cagcgtggat gcgaggtgac cgaggccggc gccggcctgc aacttctccg ccatcggcag
     4261 cggcgcgccc atctcgtcgg agacggcggc gccgatatcc ttcatgcggg tcctgtagac
     4321 ctcgatgatt ttcgagagca gcgcgacccg ctcctcgcgg ctggtccgcg aaaaggtctc
     4381 gaaggcgcgc ctggctgccg ccaccgcctt gtcgacgtcg gccttggagc cgagcgcaac
     4441 ctcatacatc gcctcttcgg tcgccgggtt gacgacgggc gtggactttt tgacggccgg
     4501 atcgacccag gcgccgtcga tgtagaattg catgcggttg accatcgtaa acctcttgtt
     4561 tcgggggcaa ttgaaggggg aacggagcgt ccggcaagca tcctcgcacg aaagcatcgg
     4621 aaaatgaacc cgccatatgc gagacgccgc actgcagcgg acgcacatat aaagtcggcg
     4681 gtgtgaagga agcaaggcgg gcgccggcat ttacatcgtc atcccggcga acgccgggat
     4741 ccatacgctg aggattctcg ttttggcacg gtggcagata ccatcaacaa caatcagcgc
     4801 caggggttat ggatcccggc gttcgccggg acgaccggct gaaaatcaca ccgccatttt
     4861 gcggtgccgc accggggcac ccgcggtcaa ggcttccgcc gcgtcgatga tggcgttggc
     4921 gtccatgccg tggtggcgat agaggtcgtc gatggtgccg gtctggccga aatgctcgac
     4981 gccgagcgcc tcgacccggt ggccgcgtac gctgccgagc cagcccaatg tggcgggatg
     5041 gccgtcgatc acggtgacga taccgcagtc gcgcggcagc ggcgccagca gttgttcgac
     5101 atggctgaga tactgcatcc cgcgccggtc gcggcgcagt tttcgcgccg cggtccatcc
     5161 cgcatgcagc cggtcggcgg acgtgatcgc cagcaggccg acatcccggc ggctttcgcc
     5221 taagaggccg atcgactcga tcgcctcggg cgctaccgcg ccggtatagg cgatcacgac
     5281 ttcggcattc ggccccggct tgcgcagcca ataggcgccg tcggtgatgg ctttctgcaa
     5341 gtcgggcgtc atgatccgct gcggctgctc gagcgttcgg gtcgaaagcc tcaaatagac
     5401 cgagccgccc tcgccggctc cctcgcgctg catgtgctgg aagccccagc ccatgatcgc
     5461 cgcgagttcg tcgacaaagg ccggctcgaa cgaggcgagg ccatcctgcg ctatgccgat
     5521 cagcggcgtc gcgatcgact gatgcgcacc gccttccggc gaaagcgtaa tgcccgacgg
     5581 cgtcgccgcc accatgaagc gcgcgtcctg atagcaggcg tagttcaggg catcgaggcc
     5641 gcgctggatg aagggatcat agagcgtcgc gatcggcagc agccgctcgc cgttgatggc
     5701 atgcgacagc cccagcgccg ccatcaggat gaacaggttc atctcggcga tgccgagctc
     5761 gatatgctgt cccttgggcg aaaagtccca gttgaaggtt gacggtatct tctcgctgcg
     5821 gaacaaatcg gccttttcgg cgcgcgcgaa cagcccgcgc cggttgaccc aggcgcccag
     5881 attggtcgac accgtgacat cgggcgacgc ggtcacgatg cgtgacgcca gctcgctgtc
     5941 gccgcgcgcg agctcgttga gcaccagccc aaaaccttgc tgggtcgaca tttgccgcga
     6001 cggcttgaac gaaagctgct cgggcacctc gatcacatca gcggtcaggc ggcgccggcc
     6061 gtcgcgattg aacggcgcct ggccaagaaa cgcctccagc gccgccggat cctgagacag
     6121 gccctcgaat ttttcccatt cgtgacccgg ccggatgttc tgcgcgc
//
LOCUS       DATNOI010000366         2281 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1454614,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000366 DATNOI010000000
VERSION     DATNOI010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454614"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..263)
                     /locus_tag="VN968_14630"
     CDS             complement(39..263)
                     /locus_tag="VN968_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30507.1"
                     /translation="MDALHLFGLFAVTAMLVCYALEDRDPVFILAFAPACALGSAYGF
                     LQGAWPFGLVEAVRAAVALRRWNVRRGVNA"
     gene            401..1336
                     /locus_tag="VN968_14635"
     CDS             401..1336
                     /locus_tag="VN968_14635"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXL30508.1"
                     /translation="MLKALMFGCSIVLLGAVGTASGQDWTKSKWGPNDEIGAANNLSP
                     DIVVKAAGLIKTGKSYGLGIETNSKTPAYPPRTFKVLVLQPGQAGGSGLGPNKTTYND
                     DIIEGWVGIGSQIDGLGHIGVDWVYYNGLKSGDFAAADGLKKLGVEKIPPIVTRGVLL
                     DMAGYLGTDLVKEGTAFNVAEIEGAAKKQGVEIRQGDVVLFHTGWVGLIGKDDKRYNS
                     GEPGLGVEGAKYLVNKGVVAIGADTWALEVIPFESKNVFEVHQILLPMSGTYILENMN
                     TGPLAADKAYEFLFVLGVPKITGAVQGIINPVAIR"
     gene            1431..1916
                     /gene="ruvX"
                     /locus_tag="VN968_14640"
     CDS             1431..1916
                     /gene="ruvX"
                     /locus_tag="VN968_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HXL30509.1"
                     /translation="MPALILPLIDASAHWPERGALVGLDLGTRTIGVAVSDPDRRLAT
                     GVETVQRSAFKADAARLLAIAGERNAVGFVLGLPINMDGSEGPRAQSTRAFARNFSKL
                     TDLTIGLWDERLSTAAVERELIGMDISRARRAKVIDEHAAIFILQGALDRLANPREMP
                     A"
     gene            complement(1923..>2281)
                     /locus_tag="VN968_14645"
     CDS             complement(1923..>2281)
                     /locus_tag="VN968_14645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HXL30510.1"
                     /translation="GFMGEVLNSARADGSLWLLVIAFVVAAPISEEFFARGFLYRGWS
                     ESFLGPAGGIMLSSLLWTALHLQYDWFFFGEVFSIGLLLGYLRYRFNSTWLTVIVHGL
                     NNLAAVAQTILLTGQG"
BASE COUNT          500 a          718 c          685 g          378 t
ORIGIN      
        1 aggctagcat tcagattagc acccacgacg gcggcaagct atgcgttcac gccccgcctg
       61 acgttccagc gtcgcagcgc gaccgccgcc ctgaccgcct caacaaggcc aaatggccac
      121 gcgccctgca gaaatccgta agccgaaccc agcgcgcagg cgggggcgaa cgcgaggatg
      181 aaaacaggat cgcgatcctc cagcgcgtaa cagaccagca ttgcggtcac cgcgaacagg
      241 ccgaacaggt gaagggcgtc catcctctgc ctccaagtct cagcgcggga ttctctggct
      301 gcatgcgcac tatcaaaaaa attggatcgg cccgctcggc gggttaggat agggcgctag
      361 ctaacaaaaa ccatgacgtc aacaaatggg aggacctgcg atgctcaaag cattgatgtt
      421 tggctgttct atcgtcttgt tgggcgcggt cggaacggca agcggacaag actggacgaa
      481 atcgaaatgg ggcccgaacg atgagatcgg cgcggccaac aatttgtcgc cggatattgt
      541 tgtgaaggcc gccggattga tcaagaccgg caagagctat ggcctcggga tcgagaccaa
      601 ttccaaaacc ccggcctatc ctccccgcac cttcaaggtg ctcgtgcttc aacccggaca
      661 agccggcggt agcggcctcg gtccgaacaa gacgacctac aacgacgaca tcatcgaggg
      721 ttgggtcggc atcggcagtc agatcgacgg cctcggccac atcggagtcg attgggtcta
      781 ttacaacgga ctcaaatcag gcgatttcgc cgcggcggac ggcttgaaaa agctcggtgt
      841 cgaaaagatc ccgccgatcg tcacccgcgg cgtgttgctc gacatggccg gctatcttgg
      901 caccgacctg gtcaaggagg gcaccgcctt caacgtcgcg gaaatcgaag gcgccgccaa
      961 gaaacagggt gtcgaaattc gtcagggcga cgtcgtcctc ttccacaccg gctgggttgg
     1021 tttgatcggc aaagacgaca aacgctacaa cagcggcgag cccgggctcg gcgtcgaagg
     1081 cgccaagtat ctggtcaaca aaggcgtcgt tgcgatcggc gccgacacct gggcgctgga
     1141 agtcatcccg tttgagtcca agaacgtgtt cgaggtgcac cagattctcc tgccgatgtc
     1201 cggcacctac attctggaga acatgaatac ggggccgctg gccgccgaca aggcctacga
     1261 gttcctgttt gtcctgggcg tgcccaagat caccggcgcc gtgcagggca ttatcaatcc
     1321 ggtggcgatc cgctgaagac gcatggccgc agcgtggcgt tcctcttttg ggagcgccac
     1381 gccgcgatcc cgctttgcgc gcactgcagc ccgtatgtta tgcgaacgcc atgcctgccc
     1441 tcattctacc gttgatcgac gccagcgcgc actggcctga acgcggcgcg ctggtcgggc
     1501 ttgatctcgg caccaggacc attggagtag cggtatccga tcccgatcgc aggctcgcga
     1561 ccggggtcga gaccgtccag cgcagcgcct tcaaggccga cgcggcgcgc ctgctcgcca
     1621 ttgccggcga acgcaacgca gttggcttcg tgctcggcct gcccatcaac atggacggca
     1681 gcgagggacc gcgtgcgcaa tccacccgcg ccttcgcccg caatttctcg aaactcaccg
     1741 atctgacgat cggcctgtgg gacgagcggc tttctaccgc cgcagtcgaa cgcgaattga
     1801 tcggcatgga catcagtcgt gcccggcgcg cgaaggtgat cgacgagcac gccgcgatct
     1861 tcatcctgca gggcgcgctc gatcggctgg caaacccgcg cgaaatgccg gcctgaaaac
     1921 cttcacccct gccccgtgag caagatggtc tgcgccaccg cggccaggtt attgaggccg
     1981 tgcacgatca ctgtcagcca ggtcgaatta aagcggtagc ggagatatcc gagcaggagg
     2041 ccgatcgaaa acacttcgcc gaaaaagaac cagtcatatt gcagatgcag cgcggtccac
     2101 agcagcgatg acagcatgat gccgccagcc gggccgagaa acgattccga ccagccacgg
     2161 tagagaaagc cgcgcgcaaa aaattcctcc gagatcggcg cggcgacgac gaatgcgatc
     2221 accagcagcc atagcgatcc atcagcgcgg gccgaattca gcacctcacc catgaaaccg
     2281 g
//
LOCUS       DATNOI010000367         8970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1683262,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000367 DATNOI010000000
VERSION     DATNOI010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683262"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..449)
                     /locus_tag="VN968_14650"
     CDS             complement(213..449)
                     /locus_tag="VN968_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HXL30511.1"
                     /translation="MTTISQSPAQPSIPNLWGGFVRWIGIGAQAVAAHWERRVAIRAL
                     LERDDRELRDIGIVRSQIEAAVGGAFNPEMGRRP"
     gene            632..1981
                     /locus_tag="VN968_14655"
     CDS             632..1981
                     /locus_tag="VN968_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HXL30512.1"
                     /translation="MSKFEYAKLADAVAAEIASGVLKPGDRLPPQRSFAYERKIATST
                     ASRVYTELLRRGLVVGEVGRGTFISGEARRGIAAATEPRGARIDLEFNYPLLPTQSAL
                     IAKSLEGLNRPEALDIALRQATSIGTMAARKVTADYLTRQNRDWSPKPEQMVFTANGR
                     QCIAAALAAVVPTGGRCGVEALTYPFIKGIAARLGVTLVPLAMDEHGVRPDAVQKAHR
                     EAHLSALYVQPIIQNPLGMTMPSSRRTDLLRVVEKLDLTVIEDAVYAFLDDEPPLAAL
                     APDRCVVLDSLSKKVAPGLSLGFVVPPLRLRENVMAAVRSGGWTASGFAFAAAQLLMG
                     DGTAAELARLKRIDAQQRQKIAVACLSGLEIQTHSKSYHLWLTLPPHWRSQAFVAAAA
                     RRDIALTPSTTFAVSPGHAPNGVRLALAAPPIHQLETALRTLAGMLNAKEEDFDSTE"
     gene            complement(2008..2601)
                     /locus_tag="VN968_14660"
     CDS             complement(2008..2601)
                     /locus_tag="VN968_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HXL30513.1"
                     /translation="MKDRHTRLPTEQEVAEINAKHLISTPIKPADLLFVFGTREDVAR
                     RVDEAFRLWREGLFRWSIVSGGATPGSELSECEIIKAAMVARGIPRERILEEHRATNT
                     GENVIFSLPILEAALGLANIRSVICLGNTWTARRYPMTLHRHWPEVEKMLISVDSFET
                     PLALWHTDPEFRARILSEWDKIEPYKAKGFIAEWPVA"
     gene            complement(2703..3293)
                     /locus_tag="VN968_14665"
     CDS             complement(2703..3293)
                     /locus_tag="VN968_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30514.1"
                     /translation="MTTDLSNETAAAIRYVYKASLIGSAHQFELTDHGLSWQVGSKSG
                     VWAYADIAAIRLSYRPVSMQSRRFRADIERKDRQRIVVLSTSWQTVTLMAPQDREYRV
                     FIIQLHGRMKQAGSRAALIGGIKSGLYAAGIAVLALVAIAITGLLARAIATGEFGGAL
                     FLVGFAALFGWQIGGFIRRNRPQTYTFDQLPAALLP"
     gene            complement(3339..3773)
                     /locus_tag="VN968_14670"
     CDS             complement(3339..3773)
                     /locus_tag="VN968_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HXL30515.1"
                     /translation="MTTGLLALTAAAIFTGAAFYVNVAEQPARLTLEDRALLTEWKPS
                     YKRGAAMQAPLALVGFVLGLMAWWQTAHPGFLIGGIAIVAPWPWTLIGIKPTNDALLA
                     TEIDRAGPPTRALVVKWGALHGVRTVLGALATVAYLWACLSG"
     gene            3870..4304
                     /locus_tag="VN968_14675"
     CDS             3870..4304
                     /locus_tag="VN968_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30516.1"
                     /translation="MAETTYFPRRLILAGAMVSGVLLALAVHMLGVGYGLDLGGLWRS
                     DTGEFMPAGAAIAWWLIASVGFCGGYFTATLMDSAVSGQIPQRMRQFLIGVGVLVLAG
                     AGQAASAPSPIPTISGVLAGLAALCLGALMAFCGAHFALREA"
     gene            complement(4361..6370)
                     /locus_tag="VN968_14680"
     CDS             complement(4361..6370)
                     /locus_tag="VN968_14680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXL30517.1"
                     /translation="MYAAPIDRRDFLRRTAALGTSLPFAGLAWKAAAQSTDIVARRVF
                     FDNPDYGSVRVSPDGQTLSFLAPIDGVRNLWVAPVADPSAARPVTRATDRNLASYYRW
                     AHTSRHLVFFQERDGDENWRASSVDIASGVIVPLTPPQGVKSFVQELDHKFPEEMLLR
                     HNARNKRYFDMFRVNLVTGKSELEFENNEYLGLISDSDFQLRLGSRLTADGTSEYFER
                     RPDGSWTPFTSVPIGDVDGTQMIDFSADGKTLYMLDSRERDKAAFFAVEMATRKATLL
                     AADDEADIVEVSFDDRRPIAARANKDRARWHAVDASAAQDLADLAKYGSGDVEIIERS
                     SDDRLVTVFYERDTASGEYALLDRQTRTVRSLFKQRKALDNVALRKMQSVIIPARDGL
                     RLNGYLTLPPTEAGVGKLPMVLLIHGGPYARDQWGFNSTHQWLANRGYAVLSVNYRGS
                     TGFGKAFIAAADREWGGRMHDDLIDAVDWAVAQGIADPRRVGFFGASYGGYSALTAAT
                     KTPEVFACIVDVFGISNLITFMATIPPYWGPWFSVWKNRLGDPDTEAGRAFLIERSPL
                     THIDRASRPILIAQGMRDVRVVAAESEQMVTALKNRGVPVTYVTFPDEGHGFVRPDNR
                     LAFYAVAEAFLAKHLGGRSQPIGHDLTGSTLKVETGGELVPGLSG"
     gene            complement(6455..7882)
                     /locus_tag="VN968_14685"
     CDS             complement(6455..7882)
                     /locus_tag="VN968_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL30518.1"
                     /translation="MRSTSLKLQEFSARSTSRPEPKPARSDLSTSLTLAAMSLGYGVV
                     QLDVTIVNTALDSIGTSLGGGVSELQWVVSSYTIAFAAFILTAGALGDRIGAKRIFMA
                     GFAIFTAASVGCALAPNAAILIAARGVQGLGAAILVPNSLALLSHAYPDEKQRGRAVG
                     IWAAGASLALISGPLLGGGLIALVGWRSIFLVNLPIGLAGLWLSWRYATETTRYRQRE
                     IDLPGQIAAIAALGCLAGAIIEGGTLGWRNPFVIAGFAASAVLAILFVVRELRAPQPM
                     LPLSLFKHRLFALASLVGLLVNVAFYGLIFVFSLYFQRVNGLSPFATGLAFLPMLGAV
                     LPVNLVAARVTERIGAPATIAIGAALSALGCLALLGLEPGTSYWEIGAQLMIIGAGLG
                     LLVPPLTSTLLGSVEKSRSGIAAGVLNSTRQTGSVLGVALFGSLAGQATAFMAGMHES
                     LMISACLLFAAGAAIWRGAATATSS"
     gene            complement(8049..8555)
                     /locus_tag="VN968_14690"
     CDS             complement(8049..8555)
                     /locus_tag="VN968_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HXL30519.1"
                     /translation="MGLFTKDIKTMNDLFVHQLQDIYYAEKQLVKALPKMAEKATDKQ
                     LKQGFLSHLDETKTHVQRLKQVFQMHGAEVKAVDCPAIDGIIEEADEVAGEVADKSVL
                     DAALINAAQAAEHYEMTRYGSLIAWAKQLGRNDCASVLQKTLVEERETDKKLTTLAES
                     KINLRAAS"
BASE COUNT         1680 a         2845 c         2788 g         1657 t
ORIGIN      
        1 aatcggtatt gcagcccatc cttcgagacg gccacttcgt ggcctcctca ggatgaggtc
       61 taacttgttg aaacacaaca acctcatgct gaggagcgag cgacgcgagc gtctcgaagc
      121 atgggcagca agcgactcac caatctctca tagtcagtac tagggtccgt gcaaccgact
      181 ggggtgcctt ggcaaccgct tgatcacggt gcttacggcc ttcttcccat ctccggattg
      241 aaggctccgc cgaccgctgc ctcgatctga ctgcgcacta ttccgatgtc gcgcaattcg
      301 cgatcatcgc gttcgagcag cgccctgatc gcgacgcgac gctcccaatg ggcggctacg
      361 gcctgcgccc caatgccgat ccagcggaca aatccgcccc acaaattggg gatcgaaggt
      421 tgtgcggggc tttgggatat cgtggtcatc gtgcttctcc aatcggcctt tcgcccggca
      481 aagccatatc gttggcgcaa cgctgctcgg cagcggcgcc ttgggcgacc tcggattgct
      541 ctcgctctcc tcggtgcaat ctcggcttga tggtgactag atgatcctgt aattgtattg
      601 gttcaagtaa aacattgctc tcggtgcaat aatgtcgaaa ttcgaatatg cgaagcttgc
      661 cgatgccgtc gccgccgaga tcgcgagcgg cgttctcaag cctggcgacc gcttgccgcc
      721 gcagcgcagt tttgcctatg agcgcaagat cgccacctcg acggccagcc gggtctacac
      781 cgaactgttg cgccgtggcc tcgtcgtcgg cgaagtcgga cggggcacgt tcatttccgg
      841 agaagcgcgc cgcggcatcg ccgcggcaac cgagccgcgc ggcgcacgca tcgatctgga
      901 attcaactat ccgctgctgc cgacgcaatc agccttgatc gcaaagagcc tggagggact
      961 gaaccggccg gaggcactcg atatcgcgtt gcgacaagcc accagcatcg gaacgatggc
     1021 tgccagaaaa gtcaccgcgg actatctcac ccgtcaaaac cgggattggt cgccaaagcc
     1081 ggagcagatg gtgttcaccg ccaatggccg gcaatgcatc gcagccgcgc ttgccgcggt
     1141 ggtgccgacc ggcggccgct gcggcgtcga ggctttgacc tacccgttca tcaaaggcat
     1201 cgccgcccgg ctcggcgtca cgctggtgcc gcttgcaatg gatgaacatg gcgttcgtcc
     1261 ggacgccgtg cagaaggccc atcgcgaagc gcatttgtcg gcgctgtacg ttcagcccat
     1321 cattcaaaat ccgctgggca tgacgatgcc gtcctcgcgg cgcactgatc tcctgcgcgt
     1381 tgtcgagaag ctcgatctca ccgtcatcga agacgccgtt tatgcttttc ttgacgacga
     1441 gccgccgctg gccgcgctcg cgccggatcg ttgcgtggtg ctcgacagcc tttccaagaa
     1501 ggtggcgccc gggctgtcgc tcggctttgt cgttccgccg cttcgcttgc gcgaaaacgt
     1561 catggctgcg gtgcgctcgg gcggctggac ggcgtcggga tttgcgttcg cggccgcgca
     1621 actgctgatg ggtgacggca cggctgccga gcttgcacgg ctgaagcgga tcgatgcgca
     1681 gcaacgccag aaaattgccg ttgcctgcct ttccggcctt gagatccaga cccacagcaa
     1741 atcctatcat ctgtggctga cgttgccgcc gcactggcgg tcccaagcat tcgtcgctgc
     1801 cgcggcccgg cgggatatcg ctttgacgcc atcgacgacg tttgccgtca gccccgggca
     1861 tgcgccgaac ggcgtgcggc tggcgttggc tgcccctccg atccaccaac tcgaaaccgc
     1921 actgcgcacg cttgcgggaa tgttgaacgc gaaggaagag gatttcgatt cgacggaata
     1981 gcaagcgagc gagcgcggtc gccgcggtca agccaccggc cactccgcaa taaagccttt
     2041 ggccttgtac ggctcgatct tgtcccattc gctgagaatc cgggccctga attccggatc
     2101 ggtgtgccag agcgccaacg gcgtttcgaa gctgtcgact gatatcagca tcttctccac
     2161 ttccggccag tgccggtgca gcgtcatcgg gtagcggcgc gccgtccagg tgttgccgag
     2221 gcagatcacg ctgcggatat tcgcaagccc gagggcggct tcgaggatgg gcagcgagaa
     2281 gatgacgttt tcgccggtgt tggtcgcgcg atgttcctcc aggatgcgtt cccgcggaat
     2341 gccgcgcgcc accatcgcgg ccttgatgat ctcgcattcc gaaagctccg agcccggtgt
     2401 cgccccgccg ctcacgatcg accagcgaaa caagccctcg cgccaaagcc tgaaggcctc
     2461 atccacacgc cgcgcgacat cctcgcgcgt gccgaacacg aacaaaagat cggcgggttt
     2521 tatcggcgtg ctgatcaaat gtttggcgtt gatctcggca acttcctgct ccgttggcag
     2581 tcgcgtgtgc cgatccttca cagtccacct cgcgtcaaag ccgaaagcgc gcggcgccac
     2641 gatgccgtgc gccggccgca tcgcgaagtc acatttcatt gcgcttggtt gcagaggaaa
     2701 gattacggca gcaacgccgc cggcagttga tcgaacgtat aggtctgcgg ccggttgcgc
     2761 cggataaagc cgccgatctg ccagccaaac agcgcggcga atccgaccag aaacaacgcg
     2821 cctccgaatt cgccggtggc gatcgcgcgt gcgagcagac ccgtgatggc gattgcgacc
     2881 agcgccagta ccgcaatccc cgccgcatag aggccggatt tgatgccgcc gatcagcgcg
     2941 gccctgcttc cggcctgctt catgcgccca tgcagctgta tgatgaagac gcggtattcc
     3001 cggtcctgcg gcgccatcaa tgtaacggtc tgccagctgg tcgagagcac gacgatgcgc
     3061 tgccggtcct tgcgttcaat atcggcacga aagcgccgcg actgcatcga caccggccgg
     3121 tacgacagac ggatcgccgc gatatcggcg taagcccaga ccccggactt gctgccgacc
     3181 tgccacgaca acccatggtc cgtcagctcg aattgatgcg ccgagccgat cagcgacgcc
     3241 ttgtagacgt agcggatcgc agcagccgtc tcgttcgaca aatcggtcgt catgggcaat
     3301 gggccatttc cgcggtatca aaaaaccatc tgtcgcgcct agcccgacag acatgcccac
     3361 aaatatgcaa ccgttgccag cgcgcccaat acggtgcgca cgccgtggag cgcgccccat
     3421 ttgaccacaa gcgcgcgggt cggcggtccc gctctatcga tctcggtcgc cagcagcgcg
     3481 tcgttggtgg gtttgattcc gatcagcgtc catggccacg gcgcgacgat cgcaatgccg
     3541 ccgatcagga agccgggatg cgcggtctgc caccacgcca tcaaacccaa aacaaacccc
     3601 accagcgcga gcggcgcctg catcgcggcc ccgcgcttgt aggaaggctt ccattcggtg
     3661 agcagcgcgc ggtcctccag tgtgagccgc gccggctgct cggcgacatt cacgtagaac
     3721 gcggcgcctg tgaagatggc ggcggccgtc agcgcaagaa gaccggtcgt catgtgtcga
     3781 gcctttcgct gggattgcca gctcaaaaga ttgcctgagc ttgccgcctc ggcttgcacg
     3841 caccgccggc tttatcttac aagcgggcca tggccgagac gacctatttt ccgcgccgcc
     3901 tgatcctcgc gggcgccatg gtctcaggcg tgctgctggc gcttgccgtg cacatgctgg
     3961 gcgtgggcta tggcctcgac ctcggcggcc tgtggcgatc cgataccggc gaattcatgc
     4021 ccgcgggcgc ggccattgcg tggtggctga tcgccagcgt cggcttctgt ggcggctatt
     4081 tcaccgcgac gctgatggac agcgcagtgt cggggcaaat tccgcaacgg atgcggcagt
     4141 tcctgatcgg cgtcggggtg ctggtgctgg cgggcgcggg tcaggcggct tccgcgccaa
     4201 gtccgatccc gacgatttca ggcgtgctcg caggtctcgc ggcgctgtgc ctcggcgcgc
     4261 tgatggcctt ctgcggcgcg cattttgcgc tgcgcgaggc gtgagccggc gcgttcgcgc
     4321 ttgagccgcc cgcccccgcg acttatccgg aaagtcctgg ttatccggaa agtcctggca
     4381 ccaactcacc accggtttcg accttgagcg ttgagccggt gagatcatgt ccgatcggct
     4441 ggctgcggcc gccgaggtgc ttggccagaa aggcctcggc gacggcataa aacgcgagcc
     4501 ggttgtccgg ccgcacgaaa ccgtgtccct catcgggaaa ggtgacgtag gtaacgggca
     4561 cgccgcggtt cttgagggcc gtcaccatct gctccgattc ggcggcgacg acccggacat
     4621 cccgcatgcc ctgcgcgatc aggatcggac gactcgcgcg gtcgatatga gtaagcggcg
     4681 aacgctcgat aagaaaggcg cggcccgctt cggtatcggg gtcgccgagc cgatttttcc
     4741 agacgctgaa ccacggaccc caatagggcg ggatcgtcgc catgaaggtg atgaggttgg
     4801 agatgccgaa cacgtcgacg atgcaggcga acacctcggg tgtcttggtt gccgccgtca
     4861 gcgccgaata gccgccatag ctggcgccaa agaagccgac gcgcctggga tcggcgatac
     4921 cctgcgcaac tgcccaatcg accgcatcga tcagatcgtc atgcatgcgc ccaccccatt
     4981 cgcggtcagc cgcggcgata aacgctttac cgaatccggt cgagccgcgg tagttgacgc
     5041 tcaacaccgc atagccccga tttgccagcc attgatgggt cgaattgaag ccccattgat
     5101 cgcgggcata ggggccgcca tggatcagca acaccatcgg cagcttgccg actcccgctt
     5161 ccgttggcgg caatgtcaga tagccattga gacgaagccc atcgcgggcc gggatgatca
     5221 ctgattgcat tttccgtaat gccacattgt cgagcgcctt gcgttgcttg aacaagctgc
     5281 ggaccgtgcg cgtttgacga tcgagcagcg catattcgcc gctcgccgtg tcacgttcat
     5341 aaaataccgt caccaagcga tcatcggaac tgcgctcgat gatttccaca tcgccggagc
     5401 catacttggc aagatccgcc agatcctgcg ccgcgctggc gtcgaccgca tgccaacgcg
     5461 cacgatcctt gttcgcgcgc gccgcgatcg ggcggcggtc gtcgaacgag acctccacga
     5521 tgtcggcttc atcgtcggcc gcgagcaagg tggcttttcg ggtcgccatc tcgacagcga
     5581 aaaacgccgc tttatcccgc tcccgcgagt cgagcatata gagtgtcttg ccgtcggcgc
     5641 tgaagtcgat catctgggtg ccgtcgacat caccgatcgg cacgctggtg aatggtgtcc
     5701 agctcccatc aggacggcgc tcgaaatatt ctgacgtgcc gtccgccgtc aggcgcgagc
     5761 ccagccggag ctgaaagtcg ctgtcggaga tcaggccgag atattcgtta ttctcgaact
     5821 ccagttcgct cttgccggtc acgagattga cccgaaacat atcgaagtat cgcttgttgc
     5881 gggcgttgtg ccggagcagc atctcttccg gaaatttgtg atccaattcc tgcacgaacg
     5941 acttgacgcc ttgcggcgga gtcagcggaa caatgacgcc gcttgcaatg tcgacgctcg
     6001 acgcgcgcca gttctcatcg ccgtcgcgct cctggaagaa aaccagatgg cggctggtgt
     6061 gtgcccaccg atagtagctt gcgagattgc ggtcggtggc gcgcgtcacg ggccgggcag
     6121 cgctcggatc cgccaccggc gccacccaaa gattacgcac gccatcgatc ggcgcgagaa
     6181 aagaaagggt ctggccatcg ggactcactc tgacgctgcc gtaatcggga ttgtcgaaga
     6241 aaacccgccg cgcgacgatg tccgttgact gtgcggcggc cttccacgcg agccctgcaa
     6301 agggcagcga ggtacccaac gcggcggtgc ggcgcaggaa atcgcggcgg tcgatcgggg
     6361 cggcgtacat gcgggcctcc gttagcggcg cttccggccg cgtcagtcgc aagattaaat
     6421 gcgacggcga atttccgcaa tcaacaaccc gagatcacga cgaggtcgcc gtggcagcgc
     6481 cgcgccagat cgccgccccg gctgcaaaca gaaggcaggc cgagatcatc agcgactcat
     6541 gcatacctgc catgaacgcg gtggcctgac cggccagcga gccgaacagc gcgacgccca
     6601 acacgctgcc ggtttgccgg gtcgagttga ggacgccggc ggcgatgccg gagcgcgact
     6661 tctcgacgct gccgagcagg gtcgaggtta gcggtggcac cagcaggcca agaccggcgc
     6721 cgatgatcat caactgggcg ccgatttccc agtagctggt gccgggctcg agcccgagca
     6781 gcgcaagaca cccgagcgcc gacagcgcgg cgccgatggc gatcgtcgcg ggcgcgccga
     6841 tccgctcggt cacgcgtgcg gcgacgagat tgaccggcaa caccgcgccc aacatgggaa
     6901 gaaaggcaag gccggtcgcg aacggcgaca agccgttgac gcgctggaag tacaggctga
     6961 agacgaagat cagcccatag aaggcgacgt tgacgagaag accgaccagc gatgccagtg
     7021 cgaacagccg atgcttgaac agcgacagcg gcagcatggg ttgcggcgcg cgcagttccc
     7081 gcacaacgaa caggatcgcc agcaccgccg atgcggcaaa gcctgcgatt acaaaaggat
     7141 tgcgccagcc cagcgtgccg ccctcgatga tcgccccggc gagacaaccg agtgccgcga
     7201 tcgccgcgat ctggcccggc aagtcgatct cgcgctgtcg gtatcgcgtg gtctcggtgg
     7261 catagcgcca gctcagccac aggccggcga gaccgatcgg cagattgacg aggaagatcg
     7321 agcgccagcc gaccagcgcg atcagtccgc cgccgagcag cggcccggag atcagcgcaa
     7381 gactggcacc ggcggcccag attcccaccg cccggccgcg ctgcttttcg tccggatagg
     7441 cgtggctgag caatgcaagc gagttcggaa ccaggatcgc cgcgccaagc ccctgcacgc
     7501 ctcgtgccgc aatcaggatg gcggcgttag gcgcaagcgc gcagccgacc gatgctgccg
     7561 tgaagatggc gaaccccgcc atgaagatcc gcttggcacc gatccgatcg cccagcgcgc
     7621 cggcggtcag aatgaaggcg gcgaaggcga tggtgtagga gctgacgacc cattgcagct
     7681 cggacacgcc gccgcccagc gaggtgccga tactgtcgag tgcggtattg acgatggtga
     7741 cgtcgagctg aaccacgccg tagcccaggc tcatggcggc gagcgtcagc gaggttgaga
     7801 ggtccgagcg ggccggtttc ggctccggcc gggacgtaga cctagccgaa aattcctgta
     7861 atttcaatga tgtgcttcgc atggatcacc tcgattttga ggaaccaacg cggcacgaaa
     7921 atttcggttt cagccggacg cttcggccgt gatcgaagtg ttctggtgaa ccacgacggc
     7981 tgaaacgaag gacgccgcgc ggacgattcc gcgcggcgtt ttgtctcagt tcatgctctg
     8041 ccttaagatc agctcgcggc gcgaagattg atcttgctct cggcgagcgt ggtcagcttc
     8101 ttgtcggttt cccgttcctc aacgagggtc ttctgcaaga cactggcgca atcgttgcgg
     8161 ccaagctgct ttgcccacgc aatcaggctg ccatagcgcg tcatctcgta atgctcggcg
     8221 gcctgggctg cgttgatcaa tgccgcgtca agcaccgatt tgtccgcgac ctcgccggct
     8281 acctcgtccg cttcctcgat gatgccgtcg atcgccgggc agtcgactgc cttcacttcg
     8341 gcgccatgca tctggaacac ctgcttgagg cgctgcacat gagttttggt ttcgtcgaga
     8401 tggctcagga atccctgctt gagctgcttg tcggttgcct tctccgccat tttcggaagc
     8461 gccttcacaa gctgcttctc ggcgtaatag atgtcctgca actgatgcac gaacaggtcg
     8521 ttcatggttt tgatgtcttt ggtaaagagt cccattgtgg atcctttttt taagggaaca
     8581 cacagcggcg ccggtgtcgt catcgccgcg ccaatatgtt cgaatgctga taggcgctaa
     8641 caggctgatc ccggggttgt tcctgccggc gccgaaaacg tggtgatgga acagggaaca
     8701 ttgcgaagga ttaacgcggg accgatccga tccgagactc aaatcctgag cgtgacgcca
     8761 tatgtagggc atgtgacagc cgcggcgatc gaaggacgaa gctggcgttg tcaaggactg
     8821 cacagcgaca cgtttttgcc ttatggatct ttgcaagtgg accgattggc cgccgcttgc
     8881 catcgatccc ttcgatgggc tttcaatagt tgccgccccg ccgggaggac gaatgcaccg
     8941 actgctgacc acgcttgggc gtggtttcaa
//
LOCUS       DATNOI010000368         5858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_188764,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000368 DATNOI010000000
VERSION     DATNOI010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188764"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /gene="dapF"
                     /locus_tag="VN968_14695"
     CDS             <1..762
                     /gene="dapF"
                     /locus_tag="VN968_14695"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXL30520.1"
                     /translation="AVASPAGVHYDQLMVLQPPRLEGTEAFIRIYNNDGSEAAACGNG
                     MRCVARRLFEDTGRTTATFETKAGLLNCWQGPSGDLYTVDMGPPKFGWKDIPLAEEFR
                     DTRSIELQIGPIDAPVLHTPSVVSMGNPHAIFWVDDVSAYDLARFGPLLENHPIFPER
                     ANITLAHIVDRDRITIRTWERGAGLTRACGSAACATAVAAARLKRTNRIVQISLPGGE
                     LAIEWRARDDHVLMTGPATFEYEGRFDPALFASVA"
     gene            764..2035
                     /gene="mtaB"
                     /locus_tag="VN968_14700"
     CDS             764..2035
                     /gene="mtaB"
                     /locus_tag="VN968_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HXL30521.1"
                     /translation="MSVDVVTFGCRLNAFESEVIAREAERAGLTDTIVINSCAVTNEA
                     VAQARQSIRRLKRERPEARIVVTGCAAQTQARMFADMAEVDRVVGNDDKMRGEAWRQA
                     RVAFDAAAGFGIACEEKIAVADIKAVREMAPHLLEGFQKGLPRVFVQVQNGCDHRCTF
                     CIIPYGRGNSRSVPMGAVVDQVRALLERGHAEIVLTGVDLTSYGADLPGAPKLGMLTK
                     QILRHVPELKRLRISSIDQVEVDRDLLDVIADDERLMPHLHLSLQSGDDLILKRMKRR
                     HSRDDAVQFCAQVRRLRPDIAFGADIIAGFPTETEEMFARSKDLVEACGLTFLHVFPY
                     SPRPGTPAARMPQVSGEVIKERAKRLRATGEAALQKRLASEIGATRQVLIESATQGRT
                     EHFMPVAIAGETPGAVRTLAITGHDGARLMV"
     gene            complement(2148..2870)
                     /locus_tag="VN968_14705"
     CDS             complement(2148..2870)
                     /locus_tag="VN968_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HXL30522.1"
                     /translation="MSESHNERIDVPELTADEIQARVLHRDGLMLVIDKPAGLPVHRG
                     PKGGANLESSFDALRFGLPRPPVLAHRLDRDTSGCLVLGRHRKATASLGLLFKHGRIS
                     KTYWAVVEGGPIEDEGVIDMPLGRLNAERGWWQKPDPDGQTAVTNWKVLGRGDGLSWL
                     ALEPVTGRTHQLRVHSAAMGWPIVGDNIYGNGPRFGEPSLHLHSREIVIPISRNKDPV
                     RVAAPAPAHLHERLRACGWNGE"
     gene            2899..3843
                     /gene="ftsY"
                     /locus_tag="VN968_14710"
     CDS             2899..3843
                     /gene="ftsY"
                     /locus_tag="VN968_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HXL30523.1"
                     /translation="MNDTTPEQPKLSWWRRLSSGLKRTSSSLGTAVADLVTKRKLDRA
                     MLDDIEDVLLRADLGTEVAARIAAAVGVGRYDKAISADDVKSVVATEVEKVLSSVAKP
                     LVIDEAQKPFVILVVGVNGSGKTTTIGKLAAKLAAEGRKVMLAAGDTFRAAAIEQLKI
                     WGERTNSPVIAAAQGSDSASLAFNALTAAREGKLDVLLVDTAGRLQNKAELMNELEKV
                     VRVIRKVDSSAPHAVLLVLDATVGQNALSQVEAFHRTAGVTGLVMTKLDGTARGGILV
                     ALAEKHKLPVHFIGVGEGVDDLAPFTARDFAQAIAGIE"
     gene            3976..4578
                     /locus_tag="VN968_14715"
     CDS             3976..4578
                     /locus_tag="VN968_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HXL30524.1"
                     /translation="MDKTAPHPLFKLATELGPLIIFFIANAKFDLFVATGAFMVAVIV
                     AMVASYMVTRHVPMMAIVTAVVVIVFGTLTLMLHDETFIKMKPTIVYTLFAAILGGGL
                     LFGRSFIAIMFDQMFNLTPRGWRILTSRWALWFLAMAVLNEIIWRTQSTDFWVTFKAF
                     GMVPLTMLFAVTQMPLIKRYHLEPASLEASEADAGDVTKG"
     gene            complement(4775..5371)
                     /locus_tag="VN968_14720"
     CDS             complement(4775..5371)
                     /locus_tag="VN968_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HXL30525.1"
                     /translation="MSEPSTSGAAPQSRRWLVALPLLGFIALAGIFLLRLHGGDPSKI
                     PSALIGRPAPQTTLPALEGLVNNGTQVPGLDPAVFKGKVSVVNVWASWCVPCHDEAPL
                     LTKLGQDKRLQFVGINYKDAADNARRFLGRYGNPFGIVGVDGNGRASIEWGVYGVPET
                     FVVGREGTIVYKMVGPVTSDNVDTVLKVEIEKALKAGS"
     gene            complement(5368..5547)
                     /gene="ccmD"
                     /locus_tag="VN968_14725"
     CDS             complement(5368..5547)
                     /gene="ccmD"
                     /locus_tag="VN968_14725"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HXL30526.1"
                     /translation="MSLGPYTSFIVTSYALVGAVVLILIFSIAIDYRRQKERLRELDA
                     SGVIRRSGRSAADIQ"
     gene            complement(5552..>5858)
                     /locus_tag="VN968_14730"
     CDS             complement(5552..>5858)
                     /locus_tag="VN968_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme transporter HemC"
                     /protein_id="HXL30527.1"
                     /translation="WRAVEDPSRAARAAAILTLVGAINLPIIKFSVDWWNTLHQPASV
                     MRIGGSSLDRAFLIPLLVMAVAFSLLFVTLHLAAMRNEILRRRVRSLQMMQASQRAV"
BASE COUNT         1068 a         1840 c         1913 g         1037 t
ORIGIN      
        1 gcggtggcgt cgccagcggg cgtgcattac gaccagctga tggtgctgca gccgccgcgc
       61 ctggagggca ccgaggcctt catccgcatc tacaacaatg acggctcgga agctgccgcc
      121 tgcggcaacg gtatgcgctg cgtggcgcgc cggctgttcg aggacaccgg ccggaccacc
      181 gccactttcg agaccaaggc cgggctgttg aattgctggc agggcccgtc cggcgatctc
      241 tacaccgtcg acatgggccc gccgaaattc ggctggaagg atattccgct ggcggaagag
      301 tttcgcgaca cacggtcgat cgagctgcag atcggcccga tcgacgcgcc ggtgctgcat
      361 acgccgtcgg tggtcagcat gggcaatccg catgcgatct tctgggtcga cgacgtcagc
      421 gcctacgatc ttgcccgctt cgggccgctg ctggaaaatc atccgatctt tccggagcgc
      481 gccaatatca cgctggcgca tatcgtcgat cgcgatcgca tcacaatccg cacctgggag
      541 cgcggcgcgg ggctgaccag ggcctgcggt tcggcggcct gcgccaccgc ggtggccgcc
      601 gcaaggctga agcgcaccaa ccgcatcgtg cagatttcgc tgcccggtgg cgaactcgcc
      661 atcgaatggc gcgcgcgcga tgaccacgtg ctgatgacgg gcccggcgac gtttgagtat
      721 gaggggcgct tcgatccggc attattcgcc agcgtcgctt aggatgagtg tcgacgtcgt
      781 caccttcggc tgccggctca acgcgttcga atccgaggtg atcgcccgcg aggcggagcg
      841 cgccgggctc accgacacca tcgtcatcaa cagttgcgcg gtcaccaacg aagctgtggc
      901 gcaggcgcgg caatcgatcc gccggctgaa gcgggagcgg ccggaagcgc ggatcgtggt
      961 gaccggctgc gcggcgcaga cccaagcccg gatgttcgct gatatggccg aggtcgatcg
     1021 cgtcgtcggc aatgacgaca agatgcgcgg cgaggcatgg cgccaggcgc gcgtcgcgtt
     1081 cgacgcggcg gctggattcg gtatcgcctg cgaagaaaag atcgcggtcg ccgacatcaa
     1141 ggcggtccgg gagatggcgc cgcatcttct ggaaggattt caaaagggcc tgccgcgggt
     1201 gttcgtgcag gtgcagaacg gctgcgatca ccgctgcacc ttctgcatca ttccctatgg
     1261 ccgcggcaat tcgcgctcgg tgccgatggg cgcggtggtc gatcaggtcc gcgcgctgct
     1321 cgagcgcggc catgccgaga tcgtgctgac cggcgtcgat ctgacgagtt acggcgcgga
     1381 tctgccgggc gcgccgaaac tgggtatgct gaccaagcag atcctgcggc acgtgcccga
     1441 gttgaaacga ttgcggatct cctcgatcga tcaggtcgag gttgacaggg atttgctcga
     1501 cgtgatcgcc gatgatgaac gtctgatgcc gcatctgcat ctgtcgctgc agtccggcga
     1561 cgatctgatc ctgaagcgga tgaagcggcg gcattcacgc gacgatgcgg ttcaattctg
     1621 cgcgcaggtg cggcggctgc ggccggacat cgcgttcggc gccgatatca tcgcgggatt
     1681 tcccaccgaa accgaggaaa tgttcgcgcg gtcgaaggac ttagtcgaag cctgcggtct
     1741 gaccttcctg cacgtgtttc cctattcacc ccgtccgggc acgccggccg cacggatgcc
     1801 gcaggtatcc ggagaagtga tcaaggaacg cgccaaacga ttgcgggcga ccggcgaggc
     1861 cgccttgcaa aagcggctgg cttcggagat cggcgcgacg cgccaggttc tcatcgagag
     1921 cgccacgcag ggccgtaccg aacatttcat gccggtggcg attgcaggcg aaacgccggg
     1981 agcggtgcgg acgcttgcga tcaccgggca tgatggcgcg cggttgatgg tgtgaggctg
     2041 tagtgcctca ttttcagccg tcattgcgag gagcgcttgc gacgaagcaa tccattcttc
     2101 ctttacgctg ggagatggat tgcttcgctt cgctcgcaat gacgatccta ctccccgttc
     2161 catccgcagg ccctgagccg ttcgtgcaaa tgggccggcg cgggggccgc cacgcgcacc
     2221 gggtccttgt tcctcgaaat cgggatcacg atctcgcggg aatgcagatg caggctcggc
     2281 tcgccgaagc gcggtccgtt gccataaata ttgtcgccga caatcggcca gcccatggcg
     2341 gcggagtgaa cgcgcaattg atgggtgcgg ccggtgaccg gctccagcgc gagccagctc
     2401 aggccatcgc cgcgccccag tactttccag ttggtgaccg cggtctggcc gtcgggatcg
     2461 ggcttttgcc accagccgcg ctcggcattg aggcggccga gcggcatgtc gatcacgccc
     2521 tcgtcctcga tcgggccgcc ttcgaccacc gcccaatagg tcttggaaat ccggccatgc
     2581 ttgaacaaca ggcccagcga cgccgtcgcc ttgcgatggc gtcccagcac gagacagccg
     2641 gaggtgtcgc ggtcgagccg gtgggcgaga accggcggtc gcggcaggcc gaatcgcaac
     2701 gcatcgaacg aggactccag attggcgccg cccttggggc cccgatgcac cggcagcccc
     2761 gcgggcttgt cgatcaccag catcagcccg tcgcgatgga gcacgcgcgc ctgaatttca
     2821 tcggcggtca actcgggaac atcgatgcgt tcgttgtgac tttcgctcat ggcgcgaaac
     2881 cgctaacaca cccccaacat gaacgatacc actccggaac aacccaagct gagctggtgg
     2941 cggcggctgt ccagcggcct gaagcgcacg tcgagttcgc tcgggacggc ggtggccgat
     3001 ctcgtcacca agcgcaagct cgaccgcgcc atgctcgacg atatcgagga cgtgctgctg
     3061 cgcgccgatc tgggaaccga agtggcagcg cgcatcgccg ccgccgtcgg cgtcggccgt
     3121 tacgacaagg cgatctcggc ggatgacgtg aaatccgtgg tcgcgaccga ggtcgagaag
     3181 gttttgtcat ccgtggcgaa gccgctggtg attgacgaag cccaaaagcc gttcgtcatt
     3241 ctcgtcgtcg gcgtcaacgg ttccggcaag accaccacca tcggcaagct cgcggccaaa
     3301 cttgccgccg aaggccgcaa ggtgatgctg gccgccggtg acacctttcg cgccgccgcg
     3361 atcgagcaac tgaagatctg gggcgagcgc accaactcgc cggtcatcgc ggccgcgcag
     3421 ggctcggatt ccgcgagcct tgccttcaat gccctcaccg cggcgcggga gggaaagctc
     3481 gacgtgctgc tggtcgacac cgcgggaagg ctgcagaaca aggcggaact gatgaacgaa
     3541 cttgaaaaag tcgttcgcgt catcaggaag gtcgattcgt cggcgccgca tgcggtgctg
     3601 ctggtcctgg acgccacggt gggacaaaat gcgctgtcgc aggtcgaggc gtttcatcgt
     3661 acggcagggg tgacgggcct cgtcatgaca aagctcgatg gcaccgcgcg cggaggaatc
     3721 ctggtggcgc tggccgagaa acacaagctg ccggtgcact tcatcggcgt cggcgaaggc
     3781 gtcgacgatc tcgcgccgtt caccgcgcgg gatttcgcgc aggcgatcgc agggattgaa
     3841 taggcctcat ccttggagac gcggctacgc cgcgcctcag gatgagggag aagccaaatg
     3901 gaatgtcctc atcccgagga ggcgcgaagc gccgtctcga aggatggggt gcgaccaatg
     3961 taatggaccg aacaaatgga caagaccgcg ccgcatccgc tgttcaagct cgcgaccgaa
     4021 ctcggtccgc tgataatttt tttcatcgcc aacgccaaat ttgatctgtt cgtcgccacc
     4081 ggcgcgttca tggtggcggt gatcgtcgcg atggtcgcat cgtacatggt gacgcgacac
     4141 gtacccatga tggcgatcgt taccgccgtc gtcgttatcg tgttcggcac cctgacactg
     4201 atgctgcacg acgagacctt catcaagatg aagccgacca tcgtctatac gctgttcgcg
     4261 gcgatccttg gcggcggcct gctgttcggc cgctccttca tcgccatcat gttcgaccag
     4321 atgttcaatc tgacgccgcg gggctggcgt atcctgacat cgcgctgggc gttgtggttt
     4381 ctcgccatgg cggtgctgaa cgagatcatc tggcgcaccc agtccaccga tttctgggtg
     4441 accttcaagg catttgggat ggtgccgctg acgatgttgt ttgcggtcac ccagatgccg
     4501 ctgatcaaac gctatcatct ggagccggcg tcactcgagg cgagcgaggc ggacgctggt
     4561 gacgtgacta aggggtgagt ctctcctccc gcttgcgggt gtgtccctcc aaggacggtt
     4621 taagtaacgc catcaagcta gcagccgtag ggtgggcata gcgaagcgtg cccaccattc
     4681 aagatcgcga tcgcatcagt ggtgggcacg gcgcagatgc gcctttgccc accctacaat
     4741 ttgctcacaa gctcctcagg atgaggtcga tgacctaaga ccccgccttc agcgccttct
     4801 cgatttcgac cttgaggacg gtgtcgacgt tgtcggatgt gaccgggccg accatcttgt
     4861 agacgatggt gccttcgcga ccgaccacga aggtctctgg cacgccatag acaccccatt
     4921 cgatcgaggc gcggccgttg ccatcgacgc cgacgatgcc gaacggattg ccgtagcggc
     4981 cgagaaagcg ccgggcgttg tcggcggcgt ccttgtagtt gatgccgaca aactgcagtc
     5041 gcttgtcctg accgagcttg gtcaaaagcg gcgcttcgtc gtggcacggc acgcaccacg
     5101 aggcccagac attgaccacg ctgaccttgc ccttgaacac ggcaggatcg agcccaggca
     5161 cctgcgtgcc gttattgacg aggccttcca gcgccggcag cgtcgtttgc ggcgccgggc
     5221 ggccgatcaa ggccgagggg atcttcgacg gatcgccgcc gtgcagccgc agcaggaaaa
     5281 ttccggccag cgcgatgaag ccgagcagcg gcagcgccac cagccagcgc cggctttgcg
     5341 gcgccgcgcc ggatgtcgag ggttcactca ttggatatcc gccgcgctgc gcccggagcg
     5401 acggataacg ccggacgcgt cgagctcgcg caagcgttct ttctggcggc ggtaatcgat
     5461 cgcgatcgag aagatcagga tcagcaccac cgctcccacc agtgcgtagg atgtcacgat
     5521 gaaggacgtg tagggaccca gcgacatcgc tctacaccgc acgctggctg gcttgcatca
     5581 tttgcaagct gcgcacccgg cggcgcagga tctcgttgcg catcgccgcc aggtgcagcg
     5641 tgacgaacaa cagcgaaaac gccaccgcca tcaccagcag cggaatcaga aaggcgcggt
     5701 cgagcgaaga tccgccgatc cgcatcaccg atgccggctg atgcagcgta ttccaccagt
     5761 ccaccgagaa tttgatgatc ggcagattga tcgcccccac cagcgtcagg atcgcggcgg
     5821 cgcgcgcggc gcgtgaggga tcttcaaccg cccgccac
//
LOCUS       DATNOI010000369         2451 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_753897,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000369 DATNOI010000000
VERSION     DATNOI010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753897"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VN968_14735"
     CDS             <1..345
                     /locus_tag="VN968_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30528.1"
                     /translation="YVIKQSNPTYQALVYPMPTSHPCVNRLVLAANNTAGSWPTPTYA
                     LGRYQLDNNGLPMRDKPLITYLIVNICLDLGKSAVMNRQSVLRPNAMIFLALHWPCEG
                     SGCANNGIGHKH"
     gene            complement(600..1214)
                     /locus_tag="VN968_14740"
     CDS             complement(600..1214)
                     /locus_tag="VN968_14740"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HXL30529.1"
                     /translation="MTYDVTIITVRPGTHPEALLRLQQQDSEVAPAGELLACWYSELG
                     ALNQILLIRSCDDESRLVAQRAAMLQSKNPFGLAEFIVGMTMDTYVSFPFMPPIVPGR
                     FGPIYEVRTYALKPDRLAKTIELWRGSVPGRNTVSPLLAAMYSATGPAIRFMHIWPYA
                     SLDERQRLRTKAVADGVWPPPGGPDQLLAQQADVFLPAEFSPLQ"
     gene            1448..>2451
                     /locus_tag="VN968_14745"
     CDS             1448..>2451
                     /locus_tag="VN968_14745"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30530.1"
                     /translation="MTKPSAMMAAAIAAAAALISMSSAQAAGTVKIGILWPLTGNAAA
                     AGQASKAAAEVAAEIVNNKHPELGNLPLAATEGLPNQGGAKLELTFVDHQGNPSLAQQ
                     LATRLITQDAAHVLMGAYQSSCSFTATAVAERYGIPFMVGESAALNITGRGFKWVFRG
                     TPIASDYAATYMRFFEDMKKQGRTIASIAVVNENTDYGTSVADSIEAAAKKNNIPVAI
                     RIPYSASSTDVSAQVLQLKEKRPDVVIFISYTSDSILYMKTMKNLDYRPPMVIGDDTG
                     FSDPSFIPAVAEIAQGAMNRSAWDIGKPGSTTYKINEMYKAKTGRDLDDTSARNMQSF
                     "
BASE COUNT          508 a          785 c          706 g          452 t
ORIGIN      
        1 tatgtaatta agcaatcaaa cccaacctat caggcacttg tttacccgat gcccacgagc
       61 cacccttgcg tgaataggct tgttttagcc gctaacaata ctgctggctc ttggccgacc
      121 ccgacatatg ctcttggtag atatcagttg gataacaatg gtctcccaat gcgcgataaa
      181 ccgttgatta cctacttaat cgtcaatatt tgtcttgatc tcggcaagtc agcggttatg
      241 aatcgacaat cagttttacg cccaaatgcg atgatctttc tggcgcttca ttggccgtgt
      301 gagggctcag gatgcgctaa taatggaatt ggccataaac attgactatt cgtgatgtcg
      361 atgatgtccg ctgtgggtca ttcacgtcat tgccgccatc ccggcgtgcc cggttcgccc
      421 aagagcggac attcggccaa tgcccgcagt acacgcccta atgcaacggc gagaattccg
      481 cgggcagcgc tgtagggtgg gcaaatcgct actgcggtct cgcgaggtgg gcacggcgct
      541 cgcacgattt gcccacgcgg acaaaccacg ccaagcgcct ttgcccaccc tacgttcgat
      601 cattgcaacg gcgagaattc cgcgggcaga aagacgtcgg cctgctgggc caggagctga
      661 tcaggaccgc cgggcggcgg ccaaacgccg tccgccaccg ccttcgtccg cagccgttgg
      721 cgctcgtcga ggctcgcgta gggccaaatg tgcatgaacc ggatcgccgg cccggtcgcg
      781 ctgtacatcg ccgcgagcag cggagagacg gtgttgcggc cgggcaccga gccccgccac
      841 agctcgatcg ttttggccag ccggtccggc ttgagcgcgt aggtgcgcac ttcatagatc
      901 ggaccaaatc ggcccggtac gatcggcggc atgaatggga aggacacgta cgtgtccatc
      961 gtcatgccga cgatgaactc ggccaggccg aacgggttct tcgattgcag cattgcggcg
     1021 cgttgcgcga ccagccgtga ctcatcatcg caagaacgga tgagcaaaat ctgattgagt
     1081 gcgccgagct ccgaatacca gcacgccaac agctcacccg ccggcgccac ctcggaatct
     1141 tgttgctgca agcgaagaag cgcctcggga tgcgttccgg gacgaaccgt gatgatcgtg
     1201 acgtcatagg tcatcgctgc ttttcctccg gcattgcatc tgccgatcgc ttccggcgcg
     1261 cgctttcggc tttggatacg tcgccgattg ccgtcgctcg cgttgcgaca tcgcgcagcg
     1321 tccaactcgc attgacactc tgccgatatc ggttcctact gttgtcaaaa ggctaatgcg
     1381 cagcagccgc gcccacaatg ccggccgcgg caacgcggcg caaaagttca gcccccggga
     1441 ggacgacgtg acgaaaccat ccgcgatgat ggcggccgcg attgctgcgg ccgcggccct
     1501 gatctcgatg tcgtcggcgc aggccgccgg gaccgtcaag atcggcattc tgtggccgct
     1561 caccggcaac gcggcggccg ccgggcaggc atcgaaggcg gcggcggaag tcgccgccga
     1621 aatcgtcaac aacaaacatc cggagttggg caatctgccg ctggccgcga ccgaaggcct
     1681 gccgaaccag ggcggcgcca agctcgagct gaccttcgtc gaccatcaag gcaacccgtc
     1741 gctcgcccaa caacttgcga cacgcctgat cacgcaggac gccgcccatg tgttgatggg
     1801 agcttatcaa tcgtcttgca gcttcacggc gacggccgtg gccgagcgct acggcattcc
     1861 cttcatggtc ggcgaatcgg ccgccctcaa catcaccggt cgcggcttca aatgggtgtt
     1921 ccgcggcacg cccatcgcca gcgattatgc cgcgacctac atgcgcttct tcgaggacat
     1981 gaagaagcaa ggcaggacga tcgcctcgat cgcggtggtc aacgaaaaca ccgactacgg
     2041 cacctcggtc gccgactcga tcgaagccgc ggcgaagaag aacaatatcc cggttgcgat
     2101 ccgaattccc tatagcgcga gctcgaccga cgtctccgcg caggtgctgc agctcaagga
     2161 gaagcggccc gacgtcgtga tcttcatcag ctacacatcg gactcgatcc tctacatgaa
     2221 gacgatgaaa aatctcgact accggccgcc gatggtgatc ggcgacgaca ccggcttttc
     2281 cgatccctcc ttcattcccg ccgtcgccga aatcgcccag ggcgcgatga accgcagcgc
     2341 ctgggatatc ggcaagcccg gcagcacgac ctacaagatc aacgagatgt acaaggccaa
     2401 gaccggccgc gacctcgacg acaccagcgc ccgcaacatg cagagcttct t
//
LOCUS       DATNOI010000370         6410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2205058,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000370 DATNOI010000000
VERSION     DATNOI010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2205058"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VN968_14750"
     CDS             complement(<1..402)
                     /locus_tag="VN968_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HXL30531.1"
                     /translation="MRIAMIGTGYVGLVSGACFADYGHQVTCVDKDGDKVEGLRRGEI
                     PIFEPGLEALVAANVKAGRLDFTTDLAAPVAEADAVFIAVGTPSRRGDGHADLSYVYS
                     AAREIAAVLAGFTVVITKSTVPIGTGDEVERL"
     gene            647..3700
                     /gene="polA"
                     /locus_tag="VN968_14755"
     CDS             647..3700
                     /gene="polA"
                     /locus_tag="VN968_14755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HXL30532.1"
                     /translation="MPKTSEKTLPKPVAVKAPAKGDHVFLVDGSSYIFRAYHALPPLN
                     RKSDGLQVNAVLGFCNMLWKLLREMPPDNRPTHLAIVFDKSEITFRNKLYPDYKAHRA
                     PAPDDLIPQFPLIREAVRAFDLPCLEQVGFEADDLIATYVREACERGAIATIVSSDKD
                     LMQLVTDCVTMYDTMKDRRIGIAEVIEKFGVPPEKVVEVQALAGDSTDNVPGVPGIGV
                     KTAAQLIVEYGDLETLLLRAGEIKQPKRREALIENAEKARISRQLVLLDDKVTLEVPL
                     DELAVHEPDARKLIAFLKAMEFSALTRRVAEYAQIDPADIEPDPGFKSGASVFAPPPS
                     GGRPDSEAVSGGSTLFDNPSSPAAPTLPLKGGSSAPRVAQGRKVDTQPLTPTSLAAAR
                     AETARKTRIDRSKYQTVRSLEELQRWIARVHDVGHFVIEAKASSIDPMQAEICGIALA
                     LGPDDAAYIPLVHKQSGDGAGLFSAGLAPDQVKADEALEALRPLLESPGILKIGFNIK
                     FNAVMFAQAGITLHNHDDVQLMSYALDAGRGSHALDQLAERYLGHAVISHGELTGSGK
                     AKLSFDQVAIDRATAYSAEDADVILRLWRVLKPRLVSERMTSVYETLERPLISMLARM
                     ERRGISIDREVLSRLSGEFAQTAARVEAEIQEIAGEPVNPGSPKQIGDIIFGKMAIPG
                     GSKTKTGAWSTSAQILDELAEAGHEFPKKILEWRQVSKLKSTYTDALPTYVHPDTHRV
                     HTTYALAATTTGRLSSNEPNLQNIPVRTEDGRKIRRAFIASPGHKLVSADYSQIELRL
                     LAEIADIPVLKQAFRDGLDIHAMTASEMFGVPIKGMPGEVRRRAKAINFGIIYGISAF
                     GLANQLGIAREEASAYIKKYFERFPGIRAYMDETRDFCRTHGYVKTLFGRKCHYPDIR
                     ASNASVRSFNERAAINARLQGTAADIIRRAMIRMEDALAQKKLLAQMLLQVHDELIFE
                     VPDDEVAATLPVVQHVMQDAPFPAVLLSLPLHVDARAADNWDEAH"
     gene            3842..4477
                     /locus_tag="VN968_14760"
     CDS             3842..4477
                     /locus_tag="VN968_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HXL30533.1"
                     /translation="MPHLTSLLGFALVSLGLVLTPGPNMIYLISRSITHGPAAGIVSL
                     GGVALGFVFYMLCAAFGITALLLAVPYAYDALRLAGAAYLLWLAWQALKPNGRSPFQV
                     RKLAVDGPRKLFATGFVTNLLNPKIAMLYLALLPQFIDPAQGSVLTQSLALGSIQIAI
                     SVSVNAMIALAAGSIALFLGTRPTWLLLQRWLMGRVLAGLALRMAFEAKRA"
     gene            complement(4658..5284)
                     /locus_tag="VN968_14765"
     CDS             complement(4658..5284)
                     /locus_tag="VN968_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXL30534.1"
                     /translation="MTIELHTWNTPNGRKISVALEEMVLPYKVITVNITKGEQMAPDF
                     LAISPNNKIPAIVDPEGPEGKRVSIFESGAILLYLGEKTGKFLPRPLSERIPVYEWLM
                     WQMGGFGPMPGQVHHFIALENENDRAYGLKRFMTETRRLYGVLDRRLTDREFVAGALS
                     VADFAILGWAWRHPRHKVELSDFPNVKRWYDTLMARPGVKRGMEAKLD"
     gene            5570..6133
                     /gene="pyrE"
                     /locus_tag="VN968_14770"
     CDS             5570..6133
                     /gene="pyrE"
                     /locus_tag="VN968_14770"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HXL30535.1"
                     /translation="MSKSASRARLAEIIRKRSFGRGEITLASGRKSDFYFNLKPTMLD
                     PEGAALLAELSYEALKDDNLDYVGGLEMGAVPLAGALAQLSWIKGHPIAAFFVRKKPK
                     EHGARLAVEGLAKGESLSGKRVVIVEDVTTTGSSALKAVEAVRDAGGEVALVFTMVDR
                     EEGATETFAQAGLAFRSLYKAGEFLKG"
BASE COUNT         1193 a         2037 c         1983 g         1197 t
ORIGIN      
        1 caggcgttcg acttcgtcgc cggtgccgat cggcacggtc gatttggtga tcaccacggt
       61 gaagccggca agcacggctg cgatctcgcg cgcggcgctg taaacatagc tcaggtcggc
      121 gtggccgtcg ccgcgccgcg acggcgtgcc gaccgcgatg aacaccgcat cggcctcggc
      181 gaccggcgcg gcgaggtcgg tggtaaaatc cagccgcccg gcctttacgt tggcagcgac
      241 cagcgcttcg agcccaggct cgaaaatcgg gatttcaccc cggcgcaagc cttccacctt
      301 gtcgccatcc ttgtcgacgc aggtgacctg atgaccgtag tccgcaaaac aggcgccgga
      361 caccagcccg acatagcctg tgccaatcat ggcaatccgc atgaaacaac cttgtaaggg
      421 ttagcgcgac cgatcgaatt tgtgaggggt cttaaagcat tttacggccg agaggaaacc
      481 ggaaacggcg ggaatcggca tgtgttcgca ctgcccactg cccgcggttg gcgcctaggg
      541 catcaggaac gtcgcaggtg acgacagcat gtattgcggg tgagtaacac caaaaagcgc
      601 gctccaaagg ctgtcaatcg gccgcaaaaa tccttaaatt tcggccatgc cgaaaacctc
      661 cgaaaaaacc ctccccaaac ccgttgccgt caaagccccc gcgaaaggcg accacgtctt
      721 tctggtcgac ggttcctcct atatttttcg cgcctatcac gcgctgccgc cgctgaaccg
      781 caaatccgac gggttgcagg tcaatgccgt gctcggcttt tgcaatatgc tgtggaagct
      841 gttgcgcgag atgccgccgg acaaccggcc aacgcacctt gccatcgtgt tcgacaaatc
      901 cgaaattact ttccgcaaca agctctatcc cgactacaag gcgcaccggg cgccggcgcc
      961 cgacgatctg atcccgcaat ttccgctgat ccgcgaggcg gtgcgcgcgt tcgacctgcc
     1021 ctgcctcgag caagttggct tcgaggccga cgatttgatc gctacctatg tgcgcgaggc
     1081 ctgcgagcgg ggcgccatcg cgaccatcgt ctcctccgac aaggacctga tgcagctcgt
     1141 aaccgactgc gtcaccatgt acgataccat gaaggaccgc cgcatcggca ttgccgaagt
     1201 gatcgagaag ttcggcgtgc cgccggagaa ggtggtcgag gtgcaggcgc tggcggggga
     1261 ttcgaccgac aacgtgccgg gcgtgccggg catcggcgtc aagaccgcgg cgcaattgat
     1321 cgtcgaatat ggcgacctgg agacgctgct attgcgcgcc ggcgagatca agcagccgaa
     1381 gcggcgcgag gcgctgatcg agaatgccga gaaggcgcgg atctcgcgcc aactggtgct
     1441 gctcgatgac aaggtcacgc tcgaggtgcc gcttgatgaa ttggcggtcc acgagcccga
     1501 cgcgcgcaag ctgatcgcct tcttgaaggc gatggaattt tccgccctca cccgccgcgt
     1561 cgccgaatat gcgcagatcg atccggcgga tatcgaaccg gatcccggct tcaagagcgg
     1621 tgcgagtgtg tttgcgcctc cgccctcggg cggcaggccg gacagcgaag ctgtcagtgg
     1681 aggaagcact cttttcgaca atcccagttc acccgccgcc ccaacgctcc cgcttaaggg
     1741 ggggagcagc gcaccgcgcg tggcgcaagg ccgcaaggtt gacactcagc ccctcacccc
     1801 gacctcgctc gccgccgccc gcgccgaaac ggcgcgcaag accaggatcg accgcagcaa
     1861 ataccagacc gttcgcagcc tcgaagagct tcagcgatgg atcgcgcggg tgcacgacgt
     1921 cggccatttc gtcatcgagg ccaaggcgag ttcgatcgat ccgatgcaag ccgagatctg
     1981 cggcattgcg ctggcgctcg ggccggatga cgccgcttac atcccgctcg tccacaagca
     2041 gtcgggcgat ggcgcgggcc tgttctccgc cggcctcgcg cccgaccagg tgaaggctga
     2101 cgaggcgctc gaggcgttgc ggccactgct ggagtcgccg ggcatcctga agatcggctt
     2161 caacatcaaa ttcaacgcgg tgatgttcgc gcaggccggc atcacgcttc acaaccatga
     2221 cgacgtgcag ttgatgtcct atgcgctcga tgccgggcgc ggctcgcacg cgctggatca
     2281 actggctgaa cgctatcttg gccacgccgt gatcagccac ggcgaactga ccggcagcgg
     2341 caaggcaaaa ctctccttcg atcaggttgc tatcgacagg gcgacggcgt attcggccga
     2401 ggacgccgat gtgatcctgc gattgtggcg cgtgctcaag ccgcgcctcg tctccgagcg
     2461 catgacgtcg gtctatgaga cgctggagcg gccgctgatt tccatgctgg cgcgcatgga
     2521 gcgccgcggc atctcgatcg accgcgaggt gctgtcgcgc ctgtcaggcg agttcgccca
     2581 gaccgcagcg cgggtcgagg ccgaaattca ggagatcgcg ggcgagccgg tcaacccggg
     2641 cagccccaag cagatcggcg acatcatctt cggcaagatg gccattcccg gcggcagcaa
     2701 gaccaagacc ggcgcgtggt cgacatcggc gcagatcctc gatgaactcg cggaagcggg
     2761 tcatgaattt cccaagaaga tcctggagtg gcggcaggtc tcgaaactga aatcgaccta
     2821 taccgacgca ctgccgactt acgtgcaccc cgacacccat cgcgtgcaca ccacctacgc
     2881 gctggcggcg accaccaccg gtcggctgtc atcgaacgag ccgaacctgc agaacattcc
     2941 ggtgcgcacc gaagacggtc gaaagatccg ccgcgccttc atcgcatccc ccggtcacaa
     3001 gctggtatcg gcggattatt cccagatcga attgcgattg ctggcggaga tcgccgacat
     3061 tcccgttttg aagcaagcct tccgcgacgg gctcgacatt cacgccatga cggcgtcgga
     3121 aatgttcggc gttccgatca agggtatgcc gggtgaagtg cggcggcggg cgaaagcgat
     3181 caatttcggc attatctacg gcatctcggc gtttggtctc gccaaccagc tcggcatcgc
     3241 ccgcgaggaa gcttccgctt acatcaagaa gtacttcgaa cgttttcccg gcatccgcgc
     3301 ctatatggac gagacgcgcg atttctgccg cacccatggc tatgtcaaaa cgctgttcgg
     3361 caggaagtgc cattatcccg atatcagggc ttccaacgcc tcggtccggt ccttcaacga
     3421 gcgcgccgcg atcaatgcgc gattgcaggg caccgccgcc gacatcattc gccgcgccat
     3481 gatccggatg gaagatgcgc tggcgcagaa aaagcttttg gcccagatgc tgctacaggt
     3541 tcacgacgaa ctgattttcg aggtgcccga cgacgaggtc gccgcgacct tgccggtggt
     3601 gcagcacgtc atgcaggacg cgccattccc ggcggtgctg ctctcgctgc cgctgcacgt
     3661 tgatgcgagg gctgcggata attgggacga ggcgcattga aagctggctg tcgtcgcccg
     3721 gcttgaccgg gcgatgacag cggttgaaga aatattcgac caacatccgc gccgtttacg
     3781 aaatggcgtc gcaggcagtt gcgcgttaga aaccgcgcat ctgccgccaa cgagtctacc
     3841 catgccgcat ctcacctcgc tgctcggctt tgccctggtc tcgctcggcc tggtgctgac
     3901 gccggggccg aacatgatct acctgatctc gcgctcgatc acgcatggac cggcggccgg
     3961 cattgtttcg ctcggcggcg tcgccctcgg ctttgtgttt tacatgctgt gcgcggcgtt
     4021 cggcatcacc gcgctgttgc tcgcggtgcc ctatgcctac gatgctctgc ggcttgcggg
     4081 cgccgcctat ctgttgtggc tggcgtggca ggcgttgaag ccgaacgggc gctcgccgtt
     4141 tcaagtgagg aagcttgcgg tcgacgggcc ccgcaagctg tttgcaacgg gttttgtcac
     4201 caacctgctc aacccgaaga ttgccatgct gtatctggcg ctattgccgc aattcatcga
     4261 tcccgcgcaa ggcagcgtgc tgacgcagtc gctggcgctc ggatcgatcc agatcgcgat
     4321 cagcgtcagc gtcaatgcca tgatcgcact cgccgccggc tcgatcgcgc tgttcctcgg
     4381 cacccggccg acctggttgt tactccagcg ttggctgatg ggcagggtgc tcgccgggct
     4441 ggcgctgcgg atggcgttcg aggcgaagcg ggcgtagaga gcttttttca cctctccccg
     4501 cctgcgggag aggtcggatc gccttgcgat ccgggtgagg gggtacaggg ctcaacacga
     4561 gttcagcatt cgcggagaga aagagcccct caccccaacc ctctccccgc aagcgcggcg
     4621 cgagggagaa gaaatgagcg gcgcatgaca aacctcctca atccagcttt gcttccatgc
     4681 cgcgcttgac gccggggcgc gccatcagcg tgtcgtacca gcgcttgaca ttggggaaat
     4741 ccgaaagctc caccttgtgg cgcggatgtc gccaggccca gccgaggatc gcgaagtcgg
     4801 cgacggacag tgcgcccgca acaaattcgc gatccgtcag gcggcgatcg agcacgccat
     4861 aaagccttcg cgtctccgtc atgaagcgtt tcaggccgta ggcgcggtcg ttttcgttct
     4921 ccagcgcaat gaagtgatgg acctgtcccg gcatcggtcc gaagccgccc atctgccaca
     4981 tcagccattc atataccggg atgcgctcgc tcagcgggcg ggggagaaat ttaccggtct
     5041 tctcgccgag atagagcagg atcgcacctg attcgaagat gctgacgcgc ttgccttcag
     5101 ggccctcggg atcgacgatc gccggaatct tgttgttcgg gctgatcgcc aggaaatcag
     5161 gcgccatctg ctcgcccttg gtgatattca ccgtgatgac cttgtacggc agcaccatct
     5221 cctccagcgc gacgctgatc ttgcggccgt tcggggtgtt ccaggtgtgc agttcgatgg
     5281 tcattttttg tcctcacaaa ggctttccgt agcccggaac ctcggaccgt tacaaccgcg
     5341 ctttcaacat gtcgtctccg cacacgcgag gatccataac caccattgcc tatggtgggc
     5401 gaaatggcgg cttcggcttg gtgcagtatc gactttggtg gatatgggtc ccggtccgcg
     5461 cttcgcttgg ccgggacgac atgtcactgt tgacggggcg acatcggctc tggcatgtgc
     5521 gcgcttccgc cgatagattg cgcccgccca aagccctaga gaaaaagcct tgtccaagtc
     5581 agcctcccgc gcccgcctcg ccgagatcat ccgcaagcgc tcgttcggcc gtggcgaaat
     5641 cacgctggcc tcgggccgca agagcgattt ttatttcaat ctcaagccga ccatgctcga
     5701 ccccgaaggc gccgcgcttc tggccgaact gtcctacgag gcgctaaagg acgacaatct
     5761 cgattatgtc ggcggactgg aaatgggcgc ggtgccgctc gccggcgcgc tcgcgcagct
     5821 ctcttggatc aagggtcatc cgatcgccgc gttcttcgtg cgcaagaaac cgaaggagca
     5881 cggcgcgcgg ctcgccgtcg aaggattggc aaagggcgag agcctgagcg gaaagcgcgt
     5941 ggtgatcgtc gaggacgtca ccacgaccgg cagttcggcg ctgaaggcgg tcgaagcggt
     6001 gcgcgatgcc ggcggcgagg tcgcactggt gttcaccatg gtcgaccgcg aagagggcgc
     6061 gaccgaaact tttgcgcagg ccggccttgc gtttcgctcg ctctacaagg ccggggaatt
     6121 cttgaagggg taagtgcagc gccgctcttg ctgctccctc gccccgcact tcgcggggag
     6181 agggttgggg tgaggggccc ttcgtcccgc gaatgctgag attgacgtat gcggagactc
     6241 cccctcaccc ggattcgcgc tttcgcgcga atccgacctc tccccgcaag cggggcgagg
     6301 tgaaggaaag acccactttc ttttttcgcc catccaccgc ctgctcatag ctcgtttacc
     6361 atcgcgcatt atggtgaatc cgggccgagg cgttcatcgc cgcggcgggt
//
LOCUS       DATNOI010000371         2273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1100686,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000371 DATNOI010000000
VERSION     DATNOI010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100686"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VN968_14775"
     CDS             <1..189
                     /locus_tag="VN968_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HXL30536.1"
                     /translation="SVVGAGAVITEGKQFPEYSLIIGAPARVIRALEPAQVEKMGGAA
                     RSYMRNGSRFKKGLKKIG"
     gene            complement(200..460)
                     /locus_tag="VN968_14780"
     CDS             complement(200..460)
                     /locus_tag="VN968_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30537.1"
                     /translation="MRKLIAFDEDTFDKLRQLARDRMASFQELADEAFADLLKKHGIP
                     IDLKDALRKSARRDARSGKAGEQHATPPKASGKRASGKRNPD"
     gene            621..>2273
                     /locus_tag="VN968_14785"
     CDS             621..>2273
                     /locus_tag="VN968_14785"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HXL30538.1"
                     /translation="MNAAPQNAETRRSSEPGWIERAATRLKRWIDNLSVAEKLYSIVA
                     LLVVVTIFLLAMSIQTVRLQSSYRHLEASSSRAANDIGRVDALIYAVVMESRGVYMST
                     DRTMVKQYAEELLKRNRELAMATLELEETVRHSDTAEFPAFKERILQFIDFREELARR
                     ALVIGAGSARAWGDNDANRELRSQLNVDLEALAKIYHERAREAAELGDLGQYACWYLF
                     GLGLAALLFAAVNLQVVRKSVIGPLSEIIQMSDRIARGDIKSEVPFFGRQDEIGCLAI
                     ALRHFRKAVEHNDELIQREFDTAKQRDAAAEERDRYSDRHLETKWQLNAAIDSIPQGM
                     IMHDSKASLLVISDRYRKMYALPASIKVGSSLQDVLQHGIDSGQFAGDAAKHLEAIMA
                     RIDKRQPTSVEVKLHDGRMIRIQESPIDGGGSVAMHEDVTEQHQRQRLLQRTKHFLAT
                     LIENIPEGIIAKDARNLRYVFVNKAAEAMIGMPRAELIGKTARELFPAETAELIERRD
                     RQLIAQKQQPEPIVDIVDNPVKGRRRIAVRRLQLDGPDDESHL"
BASE COUNT          485 a          697 c          677 g          414 t
ORIGIN      
        1 agcgtggttg gcgcgggcgc ggtcatcacc gaaggaaagc agtttcccga atattccttg
       61 atcatcggtg cgccggcgcg ggtgatccgc gcgctcgagc ccgcccaggt cgagaagatg
      121 ggcggcgccg cccgatccta catgcgcaac gggtcccgct tcaaaaaggg gctcaagaag
      181 atcggctgaa aaccgaacct caatccgggt tccgtttgcc agatgccctc ttgcccgacg
      241 ccttcggcgg cgtagcgtgt tgctcgcccg ccttgccgga tcgcgcgtca cgtcgcgcgc
      301 tcttgcgcag cgcgtccttc agatcgatgg ggatgccgtg cttcttcagg agatcggcga
      361 acgcttcgtc cgccagttcc tggaaactcg ccatcctgtc gcgcgccaac tgcctgagct
      421 tgtcgaacgt atcctcgtcg aacgcgatca atttacgcat ggattgcccg tttctctgcg
      481 caatgtcgac agagccatag acgagcagca aaaactggcc ccggtcaact acggccgtat
      541 tcctaccagt cgccaattta aggttgcaat agcgttacca tagctaaaaa accgatcagc
      601 cctcgacgaa gagcaggttg atgaacgccg ccccccagaa tgccgaaacc aggaggtctt
      661 ccgaaccagg ttggatcgag agagccgcga caagactcaa gcgctggatt gacaatctaa
      721 gcgtcgccga gaagctttac agcatcgttg cgttgctggt cgtcgtcacg atcttccttc
      781 tcgccatgtc aattcaaacg gtacggctgc aatccagcta tcggcacctg gaggcgtcat
      841 cctccagggc ggcaaacgac atcggccggg tcgacgccct gatctacgcc gtcgtcatgg
      901 agtcgcgcgg cgtctacatg tcaacggacc gcacgatggt gaagcaatat gccgaagagc
      961 ttctgaagcg taaccgcgaa ctcgccatgg cgaccctcga actggaagaa accgtacgcc
     1021 acagcgatac ggcggagttt cctgccttca aggaacgaat tctgcagttc atcgatttcc
     1081 gcgaggagct tgcgcggcgc gcactggtca tcggggcggg atcggcccgc gcgtggggcg
     1141 acaacgacgc gaaccgcgag ttgcggagcc agctcaatgt tgatcttgaa gcccttgcga
     1201 agatttatca cgagcgcgcc cgcgaggcgg cggagctcgg cgacctcggc caatatgcgt
     1261 gctggtatct gttcggactg ggacttgccg ccctgctgtt cgccgcggtc aacctgcagg
     1321 tcgtgaggaa gtcggtgatc ggacctttat ctgaaatcat tcagatgtcg gaccggattg
     1381 cccgcggcga tatcaaatcc gaggttcctt ttttcggccg ccaggacgag attggatgcc
     1441 tcgccatcgc cttgcggcat ttccgaaagg cggtcgagca caatgacgaa ctgatacaac
     1501 gcgagtttga caccgcgaag cagcgcgacg ccgccgcgga ggaacgcgac agatactctg
     1561 acagacacct tgagacaaaa tggcagctca acgcggcgat cgacagcatc ccgcagggca
     1621 tgattatgca cgattccaag gccagtctgc tcgtgatcag cgaccgctat cggaaaatgt
     1681 acgcgttgcc tgcgagcatc aaggtggggt cttcgctcca ggacgtcctg cagcacggga
     1741 tcgacagtgg gcagttcgcc ggcgacgccg cgaaacacct ggaggcgatc atggcgcgca
     1801 tcgacaagcg gcagccgacc tccgttgagg tcaagcttca cgacgggcgc atgatccgca
     1861 tccaggagag cccgatcgat ggcggcggct cggtggcgat gcatgaggac gttaccgagc
     1921 agcatcaacg ccaacggctc ctgcagcgga ccaaacattt cctggcgaca ctcatcgaga
     1981 acatccccga aggcatcatt gccaaggacg cgcgcaatct gcgttacgtc tttgtcaaca
     2041 aggccgccga ggcgatgatc ggcatgccgc gcgccgaatt gatcggaaag accgcccgcg
     2101 aattgttccc cgcagagacc gccgaactga tcgaacgccg cgatcggcag ttgatcgcgc
     2161 agaaacagca gcccgaaccc atcgtcgata tcgtggataa tccggtcaag ggccggcgca
     2221 ggatcgcggt gcggcggctt cagctcgacg gccccgacga tgaatcgcat ctc
//
LOCUS       DATNOI010000372        16321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1441423,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000372 DATNOI010000000
VERSION     DATNOI010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 16321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16321
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441423"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VN968_14790"
     CDS             complement(<1..739)
                     /locus_tag="VN968_14790"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL30539.1"
                     /translation="MTGYIEPMVGRYIHVEIDREMHRIYFEESGAGIPLVCLHTAGSD
                     GRQWRHLLADEEFTKTFRIIAFDLPWHSKSNPPPSWTGEEYRLTTARYTATVRAFCKA
                     LALEKPIVMGCSIGGRIVLNLAIDHAAEFRALIGLEAADFQAPWYDTNWLHRPDVHGG
                     EVCAALVSGLIAPQSPESSRRETLWAYMQGGPGVFKAICISTGSMATCAGGLTRSIQA
                     SVRFICSPANMIFPARRKTRCGRLPRSA"
     gene            complement(736..1155)
                     /locus_tag="VN968_14795"
     CDS             complement(736..1155)
                     /locus_tag="VN968_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30540.1"
                     /translation="MTPAPNLIDDAGIIQAFAGIPALLEQDPALIYRGRTLDCECLLG
                     PVDHPFHVSIRGGSIVDLTPAPVLMRSWRFSYRASAVAWAEYWKPAPRPGWHDLLALT
                     KREEATLEGDLHPFMAHLQYFKDVLALPRLHRSAVPT"
     gene            complement(1361..2743)
                     /locus_tag="VN968_14800"
     CDS             complement(1361..2743)
                     /locus_tag="VN968_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXL30541.1"
                     /translation="MTRADAIDRAHQYLHSGEFLTELDRRVAYRTESQNPERRDALRA
                     YLEQELQPAFSQLDFSTRLVESPTGKGPYLVAEYRENSSAPTVLMYGHGDVVDGMAGE
                     WRDNLDPWRTTTSGNRVYGRGTADNKGQHSINLSALRAVREARSGWLGFNAKFIVETG
                     EEIGSPDLRQVCEALRDELEADLFLGSDGPRLSADRPTIFLGCRGGIRIHLDVNLRDG
                     SHHSGNWGGVLANPATILAGAIATLVDGKGRLLLDELKPPRISNQIRAALADVKIEPM
                     PDEPALSENWGEEGLSAAERLYAWNTLEVLAMSSGSIGKPANAIPGRAQAVLQLRFVV
                     GTRVDKIVDAIRARLHGIGFPMVEVTATQSFDASRTDFDSPWVGWAAESIRQTTGKAP
                     AVLPNFGGSLPNDVFSDCLGLPTIWVPHSYPGCSQHAPDEHILLSVTEEALGIMAGLF
                     WDLGEMPRAL"
     gene            2942..4318
                     /locus_tag="VN968_14805"
     CDS             2942..4318
                     /locus_tag="VN968_14805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXL30542.1"
                     /translation="MKKEIRLNAFAMNCVAHQSPGLWTHPRDRTSEYNRLPYWIDLAR
                     TLERGRFDGLFLADVLGVYDVFGGSPDAALRNATQTPANDPLLVIPAMAAVTRNLGFG
                     VTSNLSYEPPYTFARRMSTLDHLTEGRIGWNVVTGYLDSAARGAGKEKQTAHDDRYDV
                     ADEYMDVVYKLWEGSWEDDAALRDRARGIFADPAKVHRVQHQGNNYRVDAIHLSEPSP
                     QRTPVLYQAGTSPRGRQFAARHAECVFMSGPSAKIIAPRVAAIRALAREAGRNPAEIL
                     MFSMMTIILGRTEAEASAKHADYVSHVSPEGAFALMSGWTGVDFSTYDLDQQVRHVQN
                     DAGRSAMDNITRGDPDRVWTVREVAQHVGIGGIGPVLVGTPDKVADDIEAWFEQTDVD
                     GLNVPFAISPGDFEDITDMLVPELVKRGRYKEAYRQGTLREKLFGSGRARLNAPHPAT
                     QYRHHAAE"
     gene            complement(4487..6145)
                     /locus_tag="VN968_14810"
     CDS             complement(4487..6145)
                     /locus_tag="VN968_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXL30543.1"
                     /translation="MNRITGRSAFLALLKDEGITHLFGNPGTTELPIMHALKDHPDLT
                     YVMAMQEGLVVAIADGFSRASGRLVACNVHVAPGLGNAMGSLYNASFTGTPMILTAGQ
                     QEQGHGLTEPLLYGPLVRMAEPLVKWAVEVTRLEDLPRIVRRAAKVATTPPTGPVFIS
                     LPGDILNSEAGIELGRSTRVDTRVKPSDEALQVLVSRILKAERPVIIAGDEIVKSNAL
                     HEAAALAMALGCPAYQQSAPYGAHFLSESPCFMGALSRAQPQVREVLSPYDLMIVLGA
                     DPLRMSVHSEVDPLPEGLAILQIGLVEWDLAKNYGAEIALKADVKETLRVLIPALKSA
                     GGAGLEARARQGIAALKSRNWTTRRATLVEQISKNSTRSPIDPDFLALQLVEAMPGNA
                     ILVDEGLTSSRQINALRPHRDRYGYHALASGGIGWGLPASVGVSMANPDRPVVCFSGD
                     GSAMYSIQALWTAAHHKLPLTVVIVNNGGYRIIKQRLLAFHGDDHYVGMDFVDPPVDF
                     TGLAKSLGLEAMRITEAKDLKSALASAFLRSGAKLIEVVVDGSL"
     gene            6346..6666
                     /locus_tag="VN968_14815"
     CDS             6346..6666
                     /locus_tag="VN968_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXL30544.1"
                     /translation="MSRVQAKADAFRAIADPTRRAILDRLRAGPANAGTLASDFQSSR
                     PAISRHLRVLREARLVVDTRIGRERVYAVNPVPLQSVAGWLEGYRAFWQASLTKLKRN
                     LEAK"
     gene            6663..7136
                     /locus_tag="VN968_14820"
     CDS             6663..7136
                     /locus_tag="VN968_14820"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXL30545.1"
                     /translation="MSEIRTGARAVADLVDGCIVACVELATSPERVFLALASPEIIDW
                     WVNPGVFDTREWTADVRVGGQWRASGIARGLPYAIEGKFLEVDPPRRLVQTWHMVGAP
                     STPSTVTYLLEPITGGTRLTVHHSGIGEPDQRSNIGAGWRTSFDRLSEILSTHLE"
     gene            7143..7529
                     /locus_tag="VN968_14825"
     CDS             7143..7529
                     /locus_tag="VN968_14825"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HXL30546.1"
                     /translation="MAYDEKTAGRVRKFLSVRPDVVEKKLMGGLCFMVKGRMCCSVSG
                     KGGLLIRVGSDAHQLMLGEPHVEPMEMRGRIMTGFVRVAPEGYRTEGGLKKWVTRGVD
                     FVVTMPSAPSTKKETRRKVREQRRMK"
     gene            complement(7688..8170)
                     /locus_tag="VN968_14830"
     CDS             complement(7688..8170)
                     /locus_tag="VN968_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXL30547.1"
                     /translation="MAKNPKLVGVSAGQCLCGNICFEIDTPARWAWHDHSLGSRRAHG
                     AAYATYVGSWRKRFRITKGKATIARFEDKLTKTVRSFCARCGTPLFYERARSPHMVNI
                     PRALFTGRTGRQPLYHIAIEELQEWAYTGEPLVPLKGFPGVVWQRSKKKKRLDHEGLF
                     "
     gene            complement(8363..9490)
                     /locus_tag="VN968_14835"
     CDS             complement(8363..9490)
                     /locus_tag="VN968_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HXL30548.1"
                     /translation="MNYPSLFSPLQIGPYQLRHRLVMAPLTRMRAERPSLAPRLLNAE
                     YYAQRTTPGGLIIAEASPVVATGFGSPGVPGIYSEHQIKGWREVVDAVHAKGGVIFLQ
                     LWHVGRVSHSSFQPGGVLPVAPSAVPISADLKTMTADGKVVSYETPRALATGEIPGVI
                     DAYRQAARNALKAGFDGVEIHGANGYLIEQFLQSHTNLRTDRYGGSIENRARFLMEVT
                     QAVIDVWGADRVGVRLSPYGVANDSGEPDPMPLYNYAIKALDPLGLAYLHFVEPRSSG
                     AGRAEVNHQNVPAAMVLFRPIWRGTLITAGGFTGETADAAISAGHADAIAFGRIFISN
                     PDLPRRLQRGFPLTPYNRATFYGGEESGYTDYPVYGELEQA"
     gene            9598..9972
                     /locus_tag="VN968_14840"
     CDS             9598..9972
                     /locus_tag="VN968_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXL30549.1"
                     /translation="MSRQSVRSLPSLSVRIDLDSEGRIGPGKIQLLENIQSCGSISAA
                     GRAMDMSYKRAWDLVDEINRICRHPAVERQTGGRNGGGAVLTPFGLSLVARYRKIERD
                     AASAVRKELLALRTDMGRPKKA"
     gene            complement(10045..11541)
                     /locus_tag="VN968_14845"
     CDS             complement(10045..11541)
                     /locus_tag="VN968_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXL30550.1"
                     /translation="MAVTQAIPMTRHPYADGSYKKMLIDGKWIDAASGKRFETHNPAT
                     GELLATVAEGDAEDINRAVAAARRAFEGPWSKVKPYERQALLLKLADLVEKNFDELST
                     LDTLDMGAPISRTRGTKQRVLGMLRYYAGQATALHGETIENSLPGDIFSYTLKEPVGV
                     VGAIIPWNGPLGASVWKIGPAIATGCTVILKPAEEAPLTSLRLAELAMEAGIPPGVVN
                     VVPGYGETAGAALASHPGVDKVAFTGSHVTGQSIVKASAGNLKRVSLELGGKSPDIVF
                     ADADLDAAVPGAAMAVFANSGQICSAGTRLFVEQQVYDEFVGRVAEYGKKLQVGNGLD
                     PNTQIGPLVSEQQLDRVTGYLDVGRQEGAKALAGGARLTEGALSKGYFVPPTVFANVQ
                     DNMRIAQEEIFGPVISAISFKDGDELIQRANATTFGLGSGVWTRDVSKAHRVAKALRA
                     GSVWVNCYQAMDPAVPFGGYKMSGYGRESGKQHLEEYLNVKAVWIKTG"
     gene            11777..12628
                     /locus_tag="VN968_14850"
     CDS             11777..12628
                     /locus_tag="VN968_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL30551.1"
                     /translation="MPHAAMSANNVAVVTGGASGLGLAAAIRFARFGMKVCIADLDSN
                     RLAEAETKLSSVAPGGAADVMVATADVSRIEDVAELEVAVRERFGGTDILMNNAGIQP
                     GSEIFGPLQNWRRVIGVNLWGVIHGSQVFAAGMIERGRPGLIINTGSKQGITTPPGDP
                     AYNVSKAGVKAFTEALQHELRNTAGGRISAHLMIPGFVFTGLTAKGRTEKPAGAWTPE
                     QTIDFMIERLDAGDFYILCPDNDVPRSLDERRILWGAGDIVENRPALSRWHPDYAAAF
                     AAFVKDS"
     gene            12886..13113
                     /locus_tag="VN968_14855"
                     /pseudo
     CDS             12886..13113
                     /locus_tag="VN968_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(13291..13497)
                     /locus_tag="VN968_14860"
     CDS             complement(13291..13497)
                     /locus_tag="VN968_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30552.1"
                     /translation="MSKQSMREEAERLIRETMERKAIVVKQGNTRIEATCGKCGASNR
                     VSAAKGATRVKFACKECGHKQETL"
     gene            complement(13678..14091)
                     /locus_tag="VN968_14865"
     CDS             complement(13678..14091)
                     /locus_tag="VN968_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HXL30553.1"
                     /translation="MARHIVARTTDIPPGGNKVVAVDGRDIVVFHVNGEFFALLNRCP
                     HEGAPLDKAACVARLTSPEPGVYQRSRVGELLRCAWHGWEFDMRNGQSYFDPARVKIR
                     TYPVVIEDGETLAKGPYVAETFPVHIEGSYVIVET"
     gene            complement(14096..15208)
                     /locus_tag="VN968_14870"
     CDS             complement(14096..15208)
                     /locus_tag="VN968_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL30554.1"
                     /translation="MSDVIDRPLPEAEAPAKNRLRIIDCDVHPSLHARSDLNPFLSKR
                     WQQHMKTFGDHLRTPYIGTTPYPRSSPLIARRDAWPPTGGPPGSDLAFMRKQHLDPLD
                     IEFGILQVLDMFIFSQQNLEFGAAIQRAVNEWQLAHWTNWEPRLKAAILVGQDDTEAA
                     VAEIDRCAKIGQYVQINVCPRANEPLGRRRYWPIFARAQELDLPLGIHVGGYGGHAPT
                     GGGWPSYYVEEHQSNAHSVAAQLSSLVIEGVPEHFPRLKIVFIEGGLGWIPATTWRMD
                     QHFDRFRDEVPHLKRRPSEYVKEHFWFTTQPIDEPDEAKHLRSLIEWIGVDRLLFSSD
                     YPHWDFDDPRFAFKTPLTEAERARIFNGNARALYKL"
     gene            complement(15232..16293)
                     /locus_tag="VN968_14875"
     CDS             complement(15232..16293)
                     /locus_tag="VN968_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL30555.1"
                     /translation="MAATRIDCDIHPAVDGTRTTLLPYLDDHWKEQVVSRAIDGLDLT
                     SYPPNMPLSGRADWRPANGKPGSDLAMVQRGAFDQLGASHAICNVLYGAQAVFDPYMA
                     AAFCKAINDWIAAEWLARDPRLRASIVVPMQAPDLAIEEIERRAGDDRFVSVLVLAQG
                     ETLLGRRHYWPVYQVAEKHKLPIAIHAGSQYRMAPSSIGWPSYRYEYYLAEAQAFQAQ
                     VLSLIYEGVFLKFPGLKVVLMESGVSWLPAFMWRANKTWRGVRVEVPWIDREPAAIMR
                     DHIRLTTQPFDAPPDAAGVADIIDMIGSDKMFLFASDYPHWQFDGDDAIPPHLPASLV
                     SRMCADNPLETFPRLSLAA"
BASE COUNT         3126 a         5113 c         5088 g         2994 t
ORIGIN      
        1 ccgccgatcg cggcagccgt ccgcatcgtg tcttccggcg tgcaggaaaa atcatattcg
       61 ccggtgagca gataaagcgg acagacgcct gtatcgatct tgtcaacccg cccgcgcagg
      121 tcgccatcga cccggtagaa atacagatcg ccttgaagac gcccggcccg ccttgcatgt
      181 aggcccacaa ggtctcccgg cgcgagcttt ccggactttg cggcgcgatc aggccagaca
      241 ccagcgccgc gcagacttcg ccgccgtgaa cgtcgggacg gtgcagccag ttcgtatcgt
      301 accagggcgc ctgaaagtca gcggcttcca ggccgatcaa ggcgcggaat tccgcggcat
      361 ggtcgatcgc aagattgagc acgatgcggc cgccgatcga gcagcccatc acgatcggct
      421 tttccagcgc cagcgccttg cagaacgcgc gaaccgtcgc ggtgtagcgc gccgtcgtca
      481 gccgatattc ctcgccggtc cagctcggtg gcggatttga tttgctatgc cacggcaggt
      541 cgaaggcaat gatgcgaaat gttttcgtga actcttcgtc ggccagcaga tgccgccact
      601 gccgtccgtc ggagcctgcg gtgtgcaggc acaccagcgg tatgcccgcg ccgctttcct
      661 cgaaatagat gcgatgcatc tcacgatcga tctcgacatg gatatagcgg ccgaccatcg
      721 gctcgatata gccagtcatg tcggcaccgc ggaacgatgc agccgcggaa gcgccagcac
      781 atccttgaaa tactgcagat gcgccatgaa cggatgcagg tcgccctcca gcgtggcttc
      841 ctcacgcttg gtcaatgcga gcagatcgtg ccagcccggc cggggcgcgg gtttccaata
      901 ctccgcccag gccaccgccg acgcgcgata tgaaaaccgc caggaccgca tcaggaccgg
      961 ggccggcgtc aaatccacga tgctgccgcc gcggatcgac acatgaaacg gatgatcaac
     1021 cggcccgagc agacattcgc agtcgagcgt gcggccgcga taaatcagcg cagggtcctg
     1081 ctcgagcagc gcggggattc cggcaaacgc ctgaattatc ccagcgtcgt cgattaggtt
     1141 cggagctggc gtcatgcgct tcaagcaggc gttttcctgc aaatgccgac attggcgagt
     1201 gaacggaata gcttccgatc ggccgtatct atggcccgaa cgtcagcctg tcgcaaggta
     1261 cgttcggagg ataccggccg ccatagctgc gtcatattcg tcttgccgcg tcgcgcatgg
     1321 cgccacaggc ctggtgtcgc accctcgccc gcgtggagtc ctacagggcg cgcggcatct
     1381 cgcccagatc ccagaacagg cctgccatga tgccgagcgc ttcctcggtg accgacagca
     1441 gaatgtgttc gtcgggcgcg tgttgggagc agccgggata ggagtgcggc acccagatcg
     1501 tcggcaagcc caggcaatcg gaaaacacat cgttgggcaa cgagccgccg aaattcggca
     1561 ggaccgcggg cgctttgcct gtggtctgcc ggatcgattc cgccgcccag ccgacccagg
     1621 gactgtcgaa atcggttcgc gaggcgtcaa agctctgtgt cgccgtcacc tcgaccatgg
     1681 gaaacccgat gccgtgcaaa cgcgccctga tcgcgtcgac gattttatcc accctggtgc
     1741 cgaccacaaa gcgaagctgc aaaaccgcct gcgcgcgtcc cggaatggcg ttggccggct
     1801 tcccgatgct gccggacgac atcgccagca cttcgagcgt gttccacgca taaagccgct
     1861 cggccgccga caggccttcc tcgccccagt tctccgacag cgccggctca tcgggcatcg
     1921 gctcgatctt gacgtcggca agcgctgcgc ggatctggtt cgaaatacgc ggcggtttca
     1981 gttcgtccag caacagccgt cccttgccgt ccaccagggt ggcgatcgcg ccggccagga
     2041 tggttgcggg attggcaagg acaccgcccc agttgcccga gtgatgcgaa ccgtcgcgca
     2101 ggttcacatc gagatgaatc cgtattccgc cgcggcagcc gaggaatatc gtggggcgat
     2161 ccgcggaaag ccggggtccg tcggagccga gaaacagatc ggcctcgagt tcgtcgcgca
     2221 acgcttcgca aacctggcgc agatcgggcg agccgatctc ctcgccggtt tcgacaatga
     2281 atttggcgtt gaagccgagc cagccgctgc gcgcctcgcg cacggcgcga agcgccgaca
     2341 ggttgatgct gtgttgtccc ttattgtcgg cggtgccgcg gccgtaaacg cgattgcccg
     2401 acgtcgtcgt gcgccacgga tccagattgt cccgccattc tcctgccatg ccgtcgacga
     2461 cgtcaccgtg tccgtacatc agaacggtgg gtgccgaaga attctcacga tactccgcga
     2521 cgagataagg gcccttgccg gtcggggatt caaccagcct tgtcgagaaa tcgagttgcg
     2581 agaatgccgg ctggagttct tgctcgaggt aggcgcgcag cgcgtcgcgg cgctccgggt
     2641 tctggctttc ggtgcggtac gcaaccctgc gatccagttc ggtgagaaac tccccggaat
     2701 gtagatactg gtgcgcgcgg tcgatggcgt cggctctggt catgtcttgc tttttccggt
     2761 cgttagcctc ttcgaattcc gccaacgtat cgagatacga tgccggccgg aagtgggcat
     2821 tacgccaaga ttttgtcttg ccaaaacctg tcggaatgca acaatttccg ccaacagcgc
     2881 ggcgcccgac cggctgcgga tgataactcg ccgtgcaagg cgtgcctcaa ggtttgcgat
     2941 catgaaaaaa gaaatccggc tcaatgcctt cgccatgaac tgcgtcgcgc atcagtcgcc
     3001 gggcctgtgg acccatccgc gcgaccgcac cagcgaatat aaccggctgc cgtactggat
     3061 cgatctggcg cggacgctgg agcgcggacg cttcgacggt ctgtttctcg ccgacgtgct
     3121 cggcgtctat gacgtgttcg gcggcagtcc ggacgcggcg ttgcgcaacg ccacgcagac
     3181 gcctgcgaac gatccgctgc tggtgatccc ggcgatggcc gcggtcacca ggaatctcgg
     3241 ttttggggtg acctccaacc tgtcctatga gccgccctat acttttgcgc ggcggatgtc
     3301 gacgctcgac catctcaccg aggggcggat cggctggaac gtggtgacgg gctatctcga
     3361 cagcgcggcc cgcggcgccg gcaaggagaa gcagaccgcg catgacgacc gttacgatgt
     3421 cgccgacgaa tatatggacg tggtctacaa gctctgggaa ggaagctggg aggacgatgc
     3481 ggcgctgcgc gatcgcgcgc gcggcatctt cgccgacccg gccaaagtgc atcgggtcca
     3541 gcaccaggga aacaattatc gggtcgacgc cattcatttg agcgaaccgt cgccgcagcg
     3601 cacccccgtg ctctatcagg ccggcacctc gccgcgcgga cggcaattcg ccgcccggca
     3661 cgccgaatgc gtgttcatgt cgggcccgtc ggccaagatc atcgcgccgc gtgttgcggc
     3721 gatccgagcg ctggcccgcg aagccggccg caacccggcc gaaatcctga tgttcagcat
     3781 gatgaccatt attctcggcc gcaccgaagc ggaagcatcc gccaaacacg ccgactatgt
     3841 cagccacgtc agcccggaag gcgcgttcgc gctgatgtcc ggctggaccg gcgtcgattt
     3901 ctcgacttac gatctcgacc agcaagtccg ccacgtccag aacgacgccg gccgcagcgc
     3961 gatggacaac atcacccgcg gcgatccgga tcgggtatgg accgtgcgcg aggtcgccca
     4021 gcatgtcggg atcggcggca tcgggccggt gctggtcggc acgcccgaca aggtcgccga
     4081 cgatatcgaa gcgtggttcg agcagaccga tgtcgacgga ttgaacgtcc cgttcgcgat
     4141 ttcgccggga gatttcgagg atatcaccga catgctggtg ccggagctgg tcaagcgcgg
     4201 acgctacaag gaggcctaca gacaaggaac gctgcgcgaa aaactgttcg gctcgggccg
     4261 cgcgcgacta aacgcaccgc atccggccac gcaatatcgc catcacgcgg cggagtaggt
     4321 tgcgatcctt cgtcatttgc gaggagcgaa gcgacgaagc aatccaagcc ctcgtcattc
     4381 cgggatggtc cgaaggacca gacctcagat gtgcaattgc acatcgggga atctcgagat
     4441 tccgggttcg atgcttcgca tcgccccgga atgacgatgg gaatccctag agcgagccgt
     4501 cgaccacgac ttcgattagt ttcgcccccg atcgtaaaaa tgccgacgcc agcgccgact
     4561 tcaaatcttt tgcctcggtg atccgcatcg cctccagccc caaggatttt gccagaccgg
     4621 taaaatccac gggtgggtcg acaaaatcca tgccgacgta gtgatcgtcg ccatgaaacg
     4681 ccagcagccg ctgcttgatg atgcggtagc cgccattgtt gacgatcacc accgtcaacg
     4741 gcagcttgtg atgcgccgcg gtccacagcg cctggatcga gtacatcgcg ctgccatcgc
     4801 cggagaaaca caccaccggc cggtcgggat tcgccatgct gacgccgacg gaagccggca
     4861 atccccagcc gatgccgccg gacgccaacg cgtgatagcc gtagcgatcg cgatgcgggc
     4921 gcagcgcgtt gatctgccgc gaggacgtca gcccctcgtc gaccaggatg gcattgcccg
     4981 gcatggcctc gaccagctgc agcgcgagaa aatccggatc gatcggtgag cgcgtgctgt
     5041 tcttcgaaat ctgctcgacc agcgtcgcgc gccgcgtcgt ccagttcctc gacttgagcg
     5101 cggcgatacc ctgcctggcg cgggcctcaa gcccagcgcc gccggcggac ttcagcgccg
     5161 gaatcagcac ccgcagggtt tccttcacat cggccttgag cgcgatctcg gcgccgtaat
     5221 tcttggcgag gtcccactcg accaggccga tctgcaggat cgccaggccc tccggcaacg
     5281 gatcgacctc gctgtgcacc gacatccgca acggatccgc gccgagcacg atcatcagat
     5341 cgtaaggcga caacacctcg cgaacctgtg gctgcgcgcg cgacagcgcc cccatgaagc
     5401 acgggctttc cgaaagaaaa tgcgcgccgt agggcgccga ttgctgatag gcgggacagc
     5461 cgagcgccat ggccagcgct gcggcttcat gcagcgcgtt gcttttgacg atttcatcac
     5521 cggcgatgat gacgggacgc tcggccttga ggatgcgcga taccagtacc tgcaaggcct
     5581 cgtcggaggg ttttacgcgg gtatcgaccc gggtcgaacg gcccagttca atgccggctt
     5641 ccgaattcag gatatcgccg ggcagcgaga tgaacaccgg accggtcggc ggcgtggtcg
     5701 cgaccttggc ggcgcggcgt acgatccgcg gcaggtcctc gagccgcgtc acctcgaccg
     5761 cccattttac cagcggttcg gccatccgga ccagcggccc atagagcagc ggctccgtca
     5821 ggccatggcc ctgctcctgt tggccggcgg tcaggatcat cggcgtgccg gtaaaactgg
     5881 cgttgtagag cgaccccatc gcgtttccga gcccgggcgc gacatggaca ttgcaggcga
     5941 cgaggcggcc cgaggcgcgg ctaaaaccgt cggcgatcgc gaccaccagg ccctcctgca
     6001 tcgccatcac ataagtgagg tcgggatggt ctttcagggc atgcatgatc ggcagttcgg
     6061 tggtgccggg attgccgaac agatgcgtga tgccctcgtc cttgagcagc gcgaggaacg
     6121 ccgagcggcc ggtgatgcga ttcatgaagt cctcccgttc cccgcagccg ttaccgtcgg
     6181 gatcacggca ccagaccaaa cttgagcggc tgacgcaatg gagcgcgatc atggctgcct
     6241 tgcacggaca cgtgcacaag ctgttgcgtc ggatgctcat gattcatgac cgaacttcga
     6301 ttcttgacgg cgggtcatct ttatgtaatc aatcggttac gcatgatgtc ccgggtacaa
     6361 gcaaaggcgg acgcttttcg cgccatcgcc gatccaaccc ggcgggctat cctcgatcgt
     6421 ctgcgagcgg ggccggcaaa tgccggcacg cttgcgtctg actttcagtc gagcaggcca
     6481 gctatttcca ggcatttgag ggtgttgcgc gaggcacggc tggtggttga cacgcggatc
     6541 gggcgcgaac gggtctatgc cgtcaatccg gtaccgctgc aaagcgtcgc cggctggctc
     6601 gaaggttatc gcgcattctg gcaggcgagt ttgacgaagc tgaagcgtaa cctggaggca
     6661 aaatgagtga gatcaggaca ggcgcacgcg cagttgcaga tttagttgac ggatgcattg
     6721 tcgcctgcgt cgaactcgct acgtcgccag agcgggtctt tctagccttg gcctcgccag
     6781 agatcatcga ctggtgggtc aatcccggcg tgttcgacac aagggaatgg accgccgacg
     6841 ttcgggtcgg cgggcaatgg cgtgcctcag gcattgcgag gggccttcct tatgcgattg
     6901 agggaaaatt cctcgaagtc gacccgccac gaagattggt acaaacgtgg catatggtcg
     6961 gggcgccgag cacgccgtcg acggtgacct atcttttgga gccgatcacc gggggcacgc
     7021 gccttacggt gcatcactcg ggcattggcg aacccgatca gcgaagcaat attggcgctg
     7081 gttggcggac cagcttcgat cgcctctcgg aaatcctgtc aactcatctc gaatagtgcg
     7141 cgatggcata cgacgaaaag acggctggac gggtgcgcaa atttctgtcg gtccggccag
     7201 acgtcgtcga aaagaagctc atgggcggtc tttgcttcat ggtgaaaggc cgcatgtgct
     7261 gttccgtcag tggaaagggc ggcctcttaa tccgcgtcgg ttcggacgcg catcagttga
     7321 tgcttggtga accgcacgtt gagccgatgg aaatgcgcgg gcgcatcatg acgggcttcg
     7381 tgcgcgtcgc tcccgagggc taccgaaccg agggcggact gaagaagtgg gtgacgcgcg
     7441 gcgtcgattt cgtggtgacg atgccttcgg ccccgtcgac gaaaaaagaa acccggcgta
     7501 aagtgaggga gcagcgacga atgaaatagg gacatcacgc cttacctccg ccatgaccgt
     7561 cgcgcgctgc gtccgctacg cagcgttcgg cgatgttcga gttcggcaca ttcgctgaga
     7621 ttaccgcaca tacgagcgtc gcttaccgac ctacggcgaa agctcgcgcg tccaattcgc
     7681 ggcctgatta aaacagcccc tcatggtcga ggcgcttttt ctttttggag cgctgccaca
     7741 ccacgccggg aaagcctttc agcggcacca gtggctcgcc ggtgtaggcc cactcctgca
     7801 gctcctcgat cgcgatgtga tagagcggct gccgcccggt gcggccggtg aacagcgcgc
     7861 gcggaatgtt caccatgtgc ggcgaacgcg cgcgttcgta aaacagcggc gtgccgcaac
     7921 gcgcacagaa gctgcgcacg gttttggtca gtttgtcctc gaagcgcgcg atcgtagcct
     7981 tgcctttggt gatgcgaaag cgcttgcgcc agcttccgac ataggtcgcg taagccgcgc
     8041 catgtgcgcg ccggctgcca agcgaatgat cgtgccaggc ccagcgcgcc ggcgtgtcga
     8101 tctcgaagca gatgtttccg cacaggcatt ggcctgcgga gactccaacc aattttggat
     8161 tttttgccat ttgcgccacc ccgtctccac cgtcattgcg aggcgcgaaa agcgacgaag
     8221 caactctccg tcattccggg atggtccgaa ggaccagacc tcggatgtgc aattgcacat
     8281 cggggaatct cgagattccg ggttcgatgc ttcgcattgc cccggaatga ccagctcact
     8341 actcgcaaat gacgtttggt tgtcaggcct gttccagctc gccatagact ggataatcgg
     8401 tatagccgga ttcctcgcct ccatagaagg tggcgcggtt ataaggtgtc aatggaaatc
     8461 cgcgctgcag ccggcgcggc aggtcgggat tggaaatgaa gatccggccg aacgcaatcg
     8521 cgtcggcatg gccggcggaa atcgccgcat ccgcggtctc gccggtaaaa ccgccggctg
     8581 tgatcaacgt gccgcgccag atcggccgaa acagtaccat ggccgcgggc acgttctgat
     8641 gattgacctc ggcgcgtccc gcaccactgc tgcgcggctc gacaaaatgc agataggcca
     8701 ggccgagcgg atcgagcgcc ttgatcgcgt agttgtacag cggcattgga tcgggctcac
     8761 cgctgtcatt ggccacgcca tagggcgaca ggcgcacgcc gacgcggtcg gcgccccaaa
     8821 cgtcgatgac ggcttgcgtg acctccatca ggaatcgcgc gcggttctcg atcgatccgc
     8881 catatcggtc ggtccgcaaa ttggtgtgcg attgcaggaa ttgctcgatc agatagccgt
     8941 tggcgccatg gatctcgacg ccgtcgaacc cggccttgag cgcgttgcgt gcggcctgcc
     9001 gataggcatc gatcacgccg ggaatctcgc cggtcgcgag cgcacgcggc gtctcatagg
     9061 acacgacctt tccgtcagcc gtcatggtct tcaagtcagc cgaaatcggc accgccgagg
     9121 gcgcgaccgg caacacgccg ccgggctgga aggaggagtg cgaaacccgt ccgacatgcc
     9181 agagctgaag aaaaatcact ccgcccttgg cgtgaacggc atcgacgacc tcgcgccagc
     9241 ccttgatctg gtgctccgaa tagataccgg gaacgccggg acttccgaaa ccggtcgcca
     9301 cgaccggcga ggcttccgcg atgatcaacc ctcccggcgt cgtgcgctgc gcataatatt
     9361 ccgcgttcag cagccggggc gccagactgg gcctttccgc ccgcattcgc gtcaagggcg
     9421 ccatcacaag gcgatgtcta agctggtacg gtccgatctg cagcggagaa aacaacgaag
     9481 gatagttcat gtacgccccg gaaaatgtgt gttgatcagg atatgtaacc gcttcgtctt
     9541 gtttgcaaaa ggcttcgatg gcaatctgaa gccccgggac ggcagagcag atcttccatg
     9601 tcaagacaaa gcgtcaggtc gctgccatcg ctgagcgttc gtatcgacct tgactcggag
     9661 ggacgcatcg ggccgggcaa aatccagctt ttggaaaata tccagagttg cggctcaatc
     9721 tcggccgcgg gccgtgcgat ggatatgtcc tacaagcggg cgtgggatct ggtggacgaa
     9781 atcaatcgta tctgccggca tcccgccgtc gaacgccaaa ccggcggcag gaacggcggc
     9841 ggcgcggtcc tgacgccgtt cggcttgtcc ctggtggcgc gttaccgcaa aatcgaacgc
     9901 gacgcggcaa gcgcggtccg caaggagctg ctggcgttgc ggacggacat ggggcgtccg
     9961 aagaaagctt gagctgctgg gataaaggcg acgggagcgg cggccggaaa ccgccactcc
    10021 tcaatatggt aaaattcgag gcgcctagcc cgtcttgatc cagaccgcct tgacgttgag
    10081 atactcttcc agatgctgct tgcctgactc gcggccatag ccgctcatct tgtagccgcc
    10141 gaacggcacc gccggatcca tcgcctgata gcaattgacc cagaccgagc cggcgcgaag
    10201 cgcctttgcc acgcgatgcg ccttgctgac gtccctggtc cagacgccgc tgcccaatcc
    10261 aaaagtcgtg gcattggcgc gctgaatcag ttcatcgcca tctttgaacg agatagccga
    10321 gatcaccggc ccaaaaattt cctcctgggc gatccgcatg ttgtcctgga cgttggcgaa
    10381 cacggtcggc ggcacgaagt aacccttcga cagcgcgcct tcggtcagcc gggcgccgcc
    10441 ggccagcgcc ttggcaccct cctggcggcc gacatcgaga tagccggtga cgcgatcgag
    10501 ctgctgctcg gagaccagcg gcccgatctg cgtgttcggg tcgaggccgt tgccgacctg
    10561 cagcttcttg ccgtattcgg cgacgcggcc gacgaattcg tcgtacacct gctgctcgac
    10621 aaacagccgc gtgccggcgc tgcagatctg gcctgaattg gcgaacaccg ccatcgccgc
    10681 acccggcacc gccgcatcga gatcggcgtc ggcgaacacg atgtccggcg atttgccgcc
    10741 gagctcgagc gacacccgct tcagattgcc cgcggacgcc ttgacgatcg attgccccgt
    10801 gacatgcgag ccggtgaaag cgaccttgtc gacgccggga tgcgaagcaa gcgcagcacc
    10861 cgcggtctcg ccatatcccg gcaccacatt gacgacgccg ggcggaatgc cggcttccat
    10921 cgccagttcg gccagccgca gcgacgtcag cggcgcttct tccgcgggct tgaggatcac
    10981 ggtgcagccg gtggcgatgg ccggaccgat cttccagacg ctggcgccga gcgggccatt
    11041 ccatggaatg atggcgccga cgacaccgac cggctccttc agcgtgtagg aaaagatatc
    11101 gccgggcagc gagttctcga tggtctcgcc gtgcagcgcg gtcgcctgcc ctgcataata
    11161 tctgagcatg ccgagcacgc gctgtttggt gccgcgggtg cggctgatcg gcgcccccat
    11221 gtccagcgta tccagcgtcg agagttcatc aaaattcttc tcgaccagat cggcaagctt
    11281 gagcagcagg gcctgccgct catatggttt gaccttgctc cacggtcctt cgaaggcgcg
    11341 gcgtgcggct gcgaccgcgc ggttgatatc ctcggcatcg ccctcggcaa cggttgccag
    11401 caattcgccg gtcgcggggt tatgggtttc gaagcgcttg ccggacgcgg cgtcgatcca
    11461 tttgccgtcg atcagcatct ttttgtagga accgtccgca taaggatgac gggtcatcgg
    11521 tattgcctgc gtcacagcca tgtctacact cccttgatat cgatcgatcg tcagaccgtc
    11581 atatccaaat atatacagcg gccacgctgc cccgtaaagg cgcgctcgcg gccggcaaaa
    11641 tgacgtcgaa ggaagaccac ccacgccgtt cgacgcatgg gtgctacgcc atattttcat
    11701 accgaatgac ggaaatatta tgtcgatctg ttcgattgtt cggcgggaca atcctcaccg
    11761 cgtcaggaga actcacgtgc cccacgccgc catgtcagcc aataatgtcg cagtcgttac
    11821 cggaggcgcg tccggattag gccttgcagc cgccatccgg ttcgctcgtt tcggtatgaa
    11881 agtttgtatc gcggatctgg attccaaccg tcttgcggaa gcggaaacca aattgtcgtc
    11941 ggtggcgccg ggtggggcgg ccgatgtcat ggtggcgact gcggacgtca gccgtatcga
    12001 agatgtggcc gaacttgaag tcgccgtgcg cgaacgattc ggcggcaccg atattctgat
    12061 gaacaacgcc ggcatccagc cgggcagcga gatattcggg ccgctgcaaa actggcggcg
    12121 cgttatcggc gtgaacctct ggggcgtgat tcacggctcg caggtctttg cagctggcat
    12181 gatcgagcgc gggcgccccg gtctcatcat caatacgggc tcgaagcagg gcatcacgac
    12241 gccaccgggc gaccccgcgt ataatgtttc gaaggcaggc gtcaaagcct tcaccgaggc
    12301 gctgcaacac gagttgcgaa acacggccgg cggccggatc agcgcgcatc tgatgattcc
    12361 gggttttgta ttcaccgggc ttaccgccaa gggccgcacc gagaagccgg ccggggcctg
    12421 gacgccggag cagaccatcg acttcatgat cgagcggctg gacgccggcg acttttacat
    12481 tttgtgcccc gataacgacg tgccccgaag tcttgacgag cggcggatcc tctggggggc
    12541 gggcgacatc gtcgaaaacc gccctgccct gtcgcgctgg catccggact acgccgcggc
    12601 ctttgcggcg ttcgtgaagg attcttagac tccgtcatcg cgagccaagc gaagcaatcc
    12661 actgacgcgg atgatccagc cagcgcaacc gtcattgcca ttggccaagc acgcattgaa
    12721 ttattcccag ggcttgtcat tcccgggcgc gcgttagcgc gagcccggga tccataacca
    12781 cgacagggtg tatggattcc ggcctgcgcc aagtggcgca tccacgatgt gcaattgcac
    12841 atcggggaat gacgatgaac gagatttgag ccacgccacc ttaatccgca aacgtcgcta
    12901 ctgaaattcg ccgagctggt caaggacatc ctgcccaggg gcgtgttgaa cgtcatcacc
    12961 gatgccaacg atctcggcga cgccatgacg aagcatccgg acattcgcaa gatctcgttc
    13021 accggttcga ccgcgaccgg ccagaaggtc atggcgagca cggcgcaaac cctgaagcgg
    13081 atcacgctgg agctgggcgg caacgacgcc ggcggcgggg ccaagcagtc gggcatcggc
    13141 accgaattcg ccgaggaagg gcttgcggag ttcacccagt tccagatcat caacgaggca
    13201 cgcacgtaac gtgacgccac gccgggctcg tccggcagga ccatcagacc gtctgcgaag
    13261 ttttgaagta agccggcggc gggccaaatt tcaaagcgtc tcttgcttgt ggccgcattc
    13321 cttgcaggcg aatttcaccc gcgtcgcgcc cttggccgcc gagacccgat ttgacgcgcc
    13381 gcattttccg caggtggcct cgatgcgggt gttgccctgc ttcaccacaa tggccttgcg
    13441 ttccatggtt tcgcggatca gacgttcggc ctcttcgcgc atcgactgct tcgacatcgg
    13501 ctgtacctgt catgaaaatc gccacgggag cagccttata acacccgctt cgcagttttt
    13561 tgacagcgat cgcgaaaaat cgcccttgaa accgaggatc gtcgcggttc caatcgtcac
    13621 gccgcctccg ggcatgatct tttccgaaaa ccggtttcca cttttcggga tcatgcctca
    13681 cgtctcgacg atgacatagc tgccctcgat gtggaccgga aacgtctcgg ccacataagg
    13741 ccctttcgcc agcgtctcgc cgtcttcgat cacgaccgga taggtgcgga tcttgacgcg
    13801 ggcgggatcg aaataggatt gcccgttgcg catgtcgaac tcccagccgt gccaggcaca
    13861 gcgcagcaat tcgccgaccc gcgagcgctg atagacaccc ggctccggcg acgtcagccg
    13921 cgccacgcat gccgccttgt cgaggggtgc gccctcatgc gggcagcggt tgagcagcgc
    13981 gaagaattcg ccgttgacat gaaacaccac gatgtcgcgg ccatcgacgg caaccacctt
    14041 gttgccgccg ggcgggatgt ctgtggtgcg ggcgacgatg tggcgcgcca tcagatcaga
    14101 gcttgtacaa cgcgcgggcg ttgccgttga aaatcctggc gcgctcggct tccgtcagcg
    14161 gcgtcttgaa cgcaaagcgc ggatcatcaa agtcccaatg cggataatcc gacgagaaca
    14221 gcaggcggtc gacgccgatc cattcgatca gcgatcgcag atgttttgct tcatccggct
    14281 cgtcgatcgg ctgggtcgtg aaccagaagt gctccttcac atattccgac ggacgccgct
    14341 tcaggtgcgg gacctcgtcg cggaatctgt caaaatgttg gtccatccgc caggtggtcg
    14401 cgggaatcca gcccagtccg ccctcgatga acacgatctt caggcgcgga aaatgctcgg
    14461 gcacgccttc gatgacgagg ctggagagtt gcgccgccac cgaatgcgcg ttcgattgat
    14521 gctcctcgac ataataggac ggccaaccgc cgccggtcgg cgcgtggccg ccataaccgc
    14581 cgacatgaat gccgagcggc aaatcaagct cctgcgcgcg agcaaaaatc ggccagtagc
    14641 ggcggcggcc gagcggctcg ttggcgcgtg gacagacatt gatctgcaca tattggccga
    14701 ttttcgcgca acgatcgatc tcggcgacgg ccgcctcggt atcgtcctgc cccaccagaa
    14761 tcgcggcttt caaccggggc tcccagttgg tccagtgcgc gagctgccat tcgttgaccg
    14821 cgcgctggat cgccgcgccg aattcgagat tctgttgcga gaagatgaac atgtcgagca
    14881 cctgcagaat gccgaactcg atatcgagcg gatcgaggtg ctgcttgcgc atgaaggcga
    14941 gatcggatcc gggcggtcct ccggtcggcg gccaggcatc gcggcgcgcg atcagcggcg
    15001 aggaccgcgg atagggcgtg gtgccgatat agggtgtgcg cagatgatcg ccaaaggtct
    15061 tcatatgctg ctgccagcgc tttgacagga acggattgag gtcggagcga gcatgcaggc
    15121 tcggatgcac atcgcagtcg atgatgcgca gccggttctt cgcaggcgct tccgcttcag
    15181 gcagcgggcg gtcgatgacg tcgctcatgc gatcctcctt cggatcaagg tttatgcggc
    15241 gagagaaagc cgcggaaaag tttcaagcgg attgtcggcg cacatgcgcg agaccaggct
    15301 tgcgggcaga tgcggcggta tcgcgtcgtc gccgtcgaat tgccagtgcg gataatccga
    15361 cgcgaacaaa aacatcttgt ccgagccgat catgtcgatg atgtcggcga cgccggccgc
    15421 atcgggcggc gcgtcgaacg gctgcgtggt caggcggatg tggtcacgca tgatcgccgc
    15481 cggctcccgg tcgatccagg gcacctcgac ccgcacgcca cgccaggtct tgttggcgcg
    15541 ccacatgaag gccggcagcc agctcacgcc ggattccatc aagaccacct tgaggccggg
    15601 aaacttcaag aacacgcctt cgtagatcag actgagcacc tgggcctgaa acgcctgcgc
    15661 ttcggcgaga taatattcgt agcgatagga cggccagccg atcgagctcg gcgccatccg
    15721 gtactgactg ccggcatgga tggcgatcgg caatttgtgt ttctcggcga cctgatagac
    15781 cggccagtag tgccgccgcc ccaaaagagt ctcgccttgc gccagcacca gcacggagac
    15841 gaacctgtcg tcgccggcgc ggcgttcgat ctcttcgatc gcgagatcag gcgcctgcat
    15901 cggcaccacg atggaagcgc gcagccgcgg gtcgcgcgcg agccattcgg ccgcgatcca
    15961 gtcgttgatc gccttgcaga acgcggccgc catataggga tcgaacaccg cctgcgcgcc
    16021 gtatagcaca ttgcagatgg catggctggc gccgagctgg tcgaacgccc cgcgctgcac
    16081 catcgcaaga tcgctgccgg gcttgccatt ggccggccgc cagtcggccc ggccggacag
    16141 cggcatgttc ggcgggtagc tggtgagatc gagcccatcg atcgcccggc tcaccacctg
    16201 ttccttccag tgatcgtcga gatagggcag cagcgtggtg cgggtgccgt ccaccgcagg
    16261 atgaatatcg cagtcgatcc gcgtggccgc catggagttt ccctgcatct tgtctttcgc
    16321 g
//
LOCUS       DATNOI010000373         3154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2549160,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000373 DATNOI010000000
VERSION     DATNOI010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2549160"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="VN968_14880"
     CDS             complement(<1..1515)
                     /locus_tag="VN968_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXL30556.1"
                     /translation="MPFASVFQRIRSFSLSIGQLTFGGFLLVLAVIMITSTASVIAIR
                     HIDATFAELQRLQSVGDLAEDIDRRMNELRLAARDFVTDPGAQSSQVGGAAASLSEIL
                     KKTRLELAPEQQDMIDGVTARLSTYRNGIERISGLISRRADLVAGLSPLREQFDAAIA
                     ETPDPVIAATLFQTQSRIASALLAHDPSAAERAAQSMRVLTISNPRLRAAVDHYADAI
                     VAISICERQIADIDREVLGSEGRLIGQVTELLREVSARRGHVLSRDFARTLTEAKWQS
                     IILGTSGIFFGLFAALFVVRRTVRPLASIAASIRALAGGEKDTSIPATDVHNEIGDIA
                     RAAEVFRRTLVDADAAREAAVHALAEQRLAEESYRKLFEGSVDGIYVTTPDGALLNAN
                     PALARIMGYETPQDLIHGIRDIADTVYIHAAARAEYETLMRRDGTVREFEYPVRARDG
                     AILWLSDSATVVRNEAGEAVRYEGTVRDITDQKRAEDAIAEGRRLLQQVIDTVPA"
     gene            complement(1692..2669)
                     /locus_tag="VN968_14885"
     CDS             complement(1692..2669)
                     /locus_tag="VN968_14885"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL30557.1"
                     /translation="MKRWRIGVIMGAWLVLLAAPGAGSAQENFPSRPVRIVIPYSAGS
                     VADVFARIVAQNMAVQWNASIIVESKSGANGSIAAEEVARAAPDGYTWLLVTTFFTAS
                     PSLYAALRWDPVRDFIPVGQICRAPNFFIVPTTLPVKNVAEYVALAKAKPGTLNFSHP
                     GKGSTGHLGFELFKRLAGIDVTAVGYRGYPQMVPDIASGLITSTFLSANQALAQVQTG
                     SIRIIGALNDGRSKYFPDVPTMAEQGYAEAQVTPWFGVVLPAATPGPVVERISKALES
                     ALAMPDVRQKLDIAGCQPMSAPLSQFADIIKADVALWAKVVKDAGITAD"
BASE COUNT          568 a         1023 c         1005 g          558 t
ORIGIN      
        1 cgccggcacg gtgtcgatca cctgctgcag cagccgccgg ccttcggcga tggcatcctc
       61 ggcgcgcttc tgatccgtga tgtcgcgcac cgtcccttca tagcggacgg cctcgccggc
      121 ctcattgcga accacggtcg cactgtcgga gagccacagg atcgcgccgt cgcgcgcgcg
      181 caccggatac tcgaactcgc gaaccgtccc gtcgcgccgc atcagtgtct cgtattcggc
      241 gcgcgccgcc gcatgaatgt aaaccgtgtc tgcaatgtcg cggatgccgt ggatcaaatc
      301 ctgcggcgtc tcatagccca tgatccgcgc cagcgccggg ttggcgttga gcagcgcgcc
      361 gtcgggcgtc gtcacgtaaa ttccgtcgac cgaaccctcg aacagcttgc gatagctctc
      421 ctcggcaagc cgctgctcgg ccagggcgtg caccgccgcc tcgcgcgcgg cgtcggcatc
      481 gaccagcgtg cggcggaata cttcggcggc acgcgcaata tcgccgatct cgttgtgcac
      541 gtcggtggcg ggaatcgatg tgtccttctc gcctcccgcc agcgcgcgga tcgaggctgc
      601 gatggatgcg agcggacgga cggtgcgtcg caccacgaag agtgccgcaa aaagtccgaa
      661 aaagatgccg gaggtgccca gtatgatgct ttgccattta gcctcggtca gggtcctcgc
      721 gaaatcacgg gacagcacgt ggccgcggcg cgcgctgacc tcacgcaaca gttcggtgac
      781 ttgcccgatc aggcgtccct ccgagcccaa aacttccctg tcgatatcgg cgatctgacg
      841 ttcgcagatc gatatcgcga cgatcgcgtc ggcgtaatgg tcgacggccg cgcgaagcct
      901 gggatttgat atcgtcagca cccgcatgct ttgagcggcc cgctcggccg cggacggatc
      961 atgcgccagc agggccgagg cgatgcggct ttgggtctga aacaacgtcg ccgcgatcac
     1021 cgggtcgggg gtctccgcaa tggccgcatc gaattgctcc cgcagcggcg acaggccagc
     1081 gaccaaatcg gcgcggcgag agatcagccc ggaaatgcgc tcgataccgt tgcgataggt
     1141 cgacaaccgc gccgtgacgc cgtcgatcat gtcctgctgc tcgggggcga gctccagccg
     1201 ggtctttttg aggatttcgc tgagcgaggc ggcggccccc ccgacctggc tggattgcgc
     1261 gcccggatcg gtcacgaagt cgcgggcggc gagccgcaat tcgttcatcc gccgatcgat
     1321 gtcctccgcg aggtcgccga cgctctgcag ccgctgcagc tcggcgaacg tcgcgtcgat
     1381 atggcggatg gcgatgacgc tggcggtgct ggtgatcatg atcaccgcca gcaccagcag
     1441 gaagccgccg aatgtcagct ggccgatcga gagcgagaac gatcgtatcc tttggaagac
     1501 actggcaaag ggcatgggta tcgggaccgg ctccggacag accttatata taccgcggat
     1561 tgaacccaaa ggggaacaga cccgcaacat cgcgacaacg gcgctgcaga ccggatcgcg
     1621 ccggccggcg aacgatcgcg gggctgctca ccatcaggta gtatcatatc aagaactcaa
     1681 cccttccggg ttcaatccgc ggtgatgccg gcgtccttga cgaccttcgc ccacagtgcg
     1741 acgtcggcct tgatgatgtc ggcgaactgc gagagcggcg cgctcatcgg ctggcatccg
     1801 gcgatgtcga gtttctgccg cacgtccggc atcgccagcg ccgattccag cgccttgctg
     1861 atgcgctcga cgaccggccc gggcgttgcc gctggcaaca cgacgccgaa ccacggcgtc
     1921 acctgcgcct cggcataccc ctgctccgcc atggtcggca cgtcggggaa atatttcgag
     1981 cggccatcgt tgagcgcgcc gatgatcctg atcgagccgg tctggacctg cgccagcgcc
     2041 tggttcgcgg acaggaaggt cgaggtgatc aggccgctcg caatatccgg taccatctgc
     2101 ggatagccgc gatagccgac cgcggtgacg tcgattccgg ccagccgctt gaacagctcg
     2161 aacccgagat gcccggtaga acccttgccg ggatggctga agttcagcgt gcctggtttt
     2221 gccttggcca gcgccacata ctcggcgaca ttcttcaccg gcaatgtggt ggggacgatg
     2281 aagaaattcg gcgcccggca tatctggccc accggaatga aatcgcgcac cgggtcccag
     2341 cgcagcgccg catacagcga ggggctggcg gtaaagaatg tcgtcaccag cagccatgtg
     2401 tagccgtcgg gggccgcgcg ggcgacttcc tcggcggcaa tcgagccatt ggcgccggat
     2461 ttggattcga cgatgatcga ggcgttccat tgcaccgcca tgttttgcgc gacgatgcgc
     2521 gcgaacacgt cggccacgct tcccgccgaa tagggaatga cgatgcgcac cggccggctg
     2581 ggaaagtttt cttgcgcaga cccagcgccg ggtgcggcga gaagcacgag ccacgccccc
     2641 atgatcacgc cgatccgcca tcgcttcatg gtgtgcgtcc cctgccctga tcgctttttg
     2701 cggcgctcga aagcgtccgg atttgccggc aggatgccga caatcccagg ggtcggcaag
     2761 agcggcggcg cggggcggtc atgatgggta tgacgggatg attcttgctt cgctgtagga
     2821 tcatcatgtc cgcgcaaaat cccgattcaa ttttcaaaca agggcgcggc gctccaattc
     2881 cctcccgccc tgcggggcga agcaatccat ctcgccgcgc aaagaaagaa tggattgctt
     2941 cgcttcgctc gcaatgacga tctctaagca tgcgtttaaa tcccgcggcg cgctgcgccc
     3001 gagtcgtgca tgaatctctc gccccaagga gggcgtgggg aatgccgggc gcccgctgca
     3061 cccgcggcct cgtgtgcact ttgtattggt agaacgcaca cgagtaacaa cgagtacacc
     3121 ggcatcaccc ggcattcccg cacgcaatgg tttt
//
LOCUS       DATNOI010000374         9178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1170638,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000374 DATNOI010000000
VERSION     DATNOI010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9178
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170638"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VN968_14890"
     CDS             complement(<1..1050)
                     /locus_tag="VN968_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HXL30558.1"
                     /translation="MASAIPSRAKILNALETLVIGTAGGVLFLWANLPGGLISGAMIA
                     VGVAAIAGRPLAVPPILTQTVLVLLGISLGSLASWQLLQHISAYPLTIGLLAVATFCS
                     TFGSSLYLQRIHGWDQTSALLAGSPGALSQITVLAAEKGADVPAIAVVQTMRVIILTA
                     ALPLVLALTGIAPSSPPAAAGAIASPLELTELIAASLAAALLLRLIKFPASWMFGAMI
                     GSSVLHGTGWIEGGLPAWVRGVALVGIGALIGARFARMKVKTLLGHVNAALGSFAIAI
                     VISAIFVAVILLTTHVRFADIVVAFAPGAMDAMLALALTLHIDPIFVGAHHLSRFVFV
                     TIATPGIVHLFGRPQE"
     gene            complement(1215..1484)
                     /locus_tag="VN968_14895"
     CDS             complement(1215..1484)
                     /locus_tag="VN968_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HXL30559.1"
                     /translation="MTTISSRVAATAFASMLLVSSAFAQTTAPATPAPAPAPKMAPAE
                     KKAEKPARTAASLECSKEADAKGLKGKERKKFRSECKKAAADKAK"
     gene            1672..2172
                     /locus_tag="VN968_14900"
     CDS             1672..2172
                     /locus_tag="VN968_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30560.1"
                     /translation="MTIHAKLLCAVAVSGFAALAITSPAQALTTQECSAKYKAAKAAG
                     TLGDQKWNDFRKAQCGADATAAPAAAPAAAPGATAAPAAPKAAEAKAKKEAAPAAAPA
                     APAGPAVYPSAVDPKYSKESAGKARMHTCVDQYNANKAANANGGLKWIQKGGGYYSEC
                     TKKLKG"
     gene            complement(2284..2490)
                     /locus_tag="VN968_14905"
     CDS             complement(2284..2490)
                     /locus_tag="VN968_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30561.1"
                     /translation="MPVFLGMILGALMLVAGVYIYDSMQTSTVANGQVAQANRTIVNW
                     DVAARDWDALKHRAHDDWVKLSSK"
     gene            2503..2751
                     /locus_tag="VN968_14910"
     CDS             2503..2751
                     /locus_tag="VN968_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30562.1"
                     /translation="MIADNAVGPGMVPFRSDPYRRHPEERALARVSKDGEGRDHPSRL
                     AEVGSHLRMTYIMNVSLLSQVRQPVLMRVPPLEAHQFV"
     gene            complement(2776..3663)
                     /locus_tag="VN968_14915"
     CDS             complement(2776..3663)
                     /locus_tag="VN968_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020487022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL30563.1"
                     /translation="MDRFDAMRVFTRVVDRRSFTLAAEDLGLPRSTVTDAVKQVEARL
                     GVRLLERTTRQVRPTLDGEAYHQRCLSLIADLEDAEAAFGGAKPKGLLRVDVHGTLAR
                     HFVMPRLPDFLAEYPGIELYMGEGDRLVDLIREGIDCVLRVGDLQDSDMIARRVAMLE
                     EITCAAPGYIDRFGLPASVDALDDDIMVGFHSSATGALLPLEFVVDGAVREVMLRTTV
                     SVNGAESLVAAARLGLGLIQVPRYHVEEDLKRGTLVAVLPDCPPSPTPVSLLYPRNRQ
                     LSPRVRVFIDWLMQAFDAP"
     gene            3786..4526
                     /locus_tag="VN968_14920"
     CDS             3786..4526
                     /locus_tag="VN968_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018857349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL30564.1"
                     /translation="MTNETNKVAIVTGASRGIGAAVAERLAGDGFTVVINYSSDAKAA
                     ETLARNIESKGGRALTAKADVSDASAVRGMFDATEAAFGGVDVLVNNAGIMKLATIAD
                     GDDALVDSHITINLKGSLNTMREAARRLREGGRIINLSSSVVGLYQPTYGVYAATKAG
                     VEAMTHVLSKELRGRNITVNAVAPGPTATALFLDGKPQAAIDHLAKLAPLERLGQPED
                     IANAVAFLAGPDGAWINGQVLRANGGII"
     gene            4574..5476
                     /locus_tag="VN968_14925"
     CDS             4574..5476
                     /locus_tag="VN968_14925"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007703377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL30565.1"
                     /translation="MSKKIILITGASSGFGRLTAEALAKAGNTVYASMRDIAGRNAKN
                     AAEMAGTLKRDGVDLRAIELDVQSEPSINGAVKRIMAESGKIDVLVHNAGHMMFGPAE
                     AFKPEQFAEQYDVNVLGTQRVNRAVLPHMRAAKQGLLVWISSSSSAGGTPPYLAPYFA
                     AKAAMDSLAVLYARELSRWGIETTIVVPGAFTKGTNHFAHSGRPADEARVAEYEAGPY
                     KGFSEEVLKAFAAIVPDEAEPSRVADEIVKVVDMPFGKRPFRIHYDPTGDGADVAFAV
                     IDRVRNEMLHRVGFSDLLKPRDNG"
     gene            complement(5487..6602)
                     /locus_tag="VN968_14930"
     CDS             complement(5487..6602)
                     /locus_tag="VN968_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXL30566.1"
                     /translation="MSEAVVDDIVAVLPPLLQSLEALGFVARHLNPPDFDSVMEAAGS
                     PDQELRAIRSRLADWPEPFASVRNSLEAASDAALAAFTGLREVQHGNGDMIAVFRALR
                     YAARAQEALYPLAAKLPPVSDFFIDPRLREDAELKARLAAPAKENTGIIHDHNEPGSR
                     GGFSLYVPEYYTPDRAWPLVMALHGGSGNGRGFLWSWLRDARCRGAILVAPTATGNTS
                     NKSTWALMGEDADTQNLGRILDSVRSRCNVDPTKLLLTGMSDGGTFCYVTGLESSSPF
                     THLAPVAATFHPLIAEMADAERLRDLPIYLVHGRLDWMFPVQVARQTHQLLSAAGADV
                     TYRELDDLSHCYPREMNAAMLDWLRGKRSTAARVARR"
     gene            complement(6742..7713)
                     /locus_tag="VN968_14935"
     CDS             complement(6742..7713)
                     /locus_tag="VN968_14935"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HXL30567.1"
                     /translation="MPSLTEWKVPPAYQPRASDYSFDLDRVLSSVVGLHSIIPPDAFS
                     ADTLGTERAGNGVVIDDGLVLTIGYLITEAEAVWLHLGDGRVVEGHALGFDSVSGFGL
                     VQALGQLGLDPLPLGSSSAAAIGDRVVVGGAGGRTRSVASHIAAKQEFAGYWEYLLDE
                     AIFTHPAHPNWGGTGLISNRGELIGIGSLQLEREREGKAEHVNMIVPIDLLKPVLDDL
                     RKFGRVNKPARPWLGMLTTEIDNRVVVIGISAKGPAARAELKAGDVILAVKGEKVSSQ
                     TAFYRKLWALGSAGVDVPLTVFHEGVTFDVVLTSTDRARLLKAPRLH"
     gene            7964..8458
                     /locus_tag="VN968_14940"
     CDS             7964..8458
                     /locus_tag="VN968_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXL30568.1"
                     /translation="MKSTRRSASSAGILMYRRVGSQLQVLLVHPGGPWWRRKDHGSWS
                     IPKGEMNAGEAAATAAIREFAEETGIVLTGPLEPLGEIRQRAGKRVFGFAVEGDLDVR
                     IVKSNIFEIEWPPGSGKMQAFPEIDRAEWFDLPLAHGKILDGQRPFLDRLAERSGQPG
                     IDRA"
     gene            8455..8877
                     /locus_tag="VN968_14945"
     CDS             8455..8877
                     /locus_tag="VN968_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HXL30569.1"
                     /translation="MTDPFDLQRFVDAQAPVYQRVLAELRQGQKQSHWIWFIFPQLAG
                     LGHSPMAQRFAIASREEAVAYLGHGVLGPRLRECTALVNAVEGRTIREILGSPDDLKF
                     CSSMTLFGAVSSNPEFAEALRKFHGGAPDRRTLDLLAR"
BASE COUNT         1773 a         2944 c         2953 g         1508 t
ORIGIN      
        1 ttcctgcggc cgtccgaaca gatggacgat gccgggcgtc gcgatggtga cgaacacgaa
       61 gcgcgacaga tgatgcgcgc cgacgaagat cgggtcgatg tgcagcgtca gcgccagcgc
      121 cagcatggcg tccatcgcgc ccggcgcgaa tgcgacgacg atgtcggcga accgcacatg
      181 ggtggtgagc aggatcaccg cgacaaagat cgcggaaatg acgatggcaa tggcaaacga
      241 tcccagcgcc gcattgacgt ggccgagcag cgttttcacc ttcatgcgcg cgaaccgcgc
      301 gccgatcagc gcgccgatcc cgaccagcgc caccccgcgc acccaggccg gcaggccgcc
      361 ctcgatccat cccgtgccgt gcagcacgct tgagccgatc attgcgccga acatccagct
      421 cgccggaaat tttatcagcc gcagcagcaa tgccgccgcc agcgatgccg cgatcagctc
      481 cgtcagctca agcggagagg cgatcgctcc cgccgcggcc ggcggcgacg acggcgctat
      541 cccggtcagc gccaagacca gcggcagcgc cgcggtgaga atgatcacgc gcatggtctg
      601 caccacggcg atggccggca cgtcggcgcc tttttctgcg gcgaggacgg tgatctgcga
      661 gagcgcgccg gggcttcccg ccaggagcgc cgaagtctgg tcccagccgt ggatgcgctg
      721 cagatagagg ctgctgccga aggtcgagca gaaggtcgcc acggccaaca gcccaatggt
      781 cagcggatag gcactgatgt gctgcagcag ttgccacgac gccagcgagc ccagcgagat
      841 cccgagcagc accagcacgg tctgggtcag gatcggcggc acggcgagcg gccggcctgc
      901 gatggcggcg acgccgaccg cgatcatggc cccggaaatc agcccgcccg gaaggtttgc
      961 ccacagaaac agcacgccgc cggcggtgcc gatgacaagc gtttcgagcg cgttgaggat
     1021 tttagcgcgg ctgggaatgg cggacgccat cgatgcaggg atcaggctca cgccgcctta
     1081 tggcaaatcc ccgcaccgcg gacaaatgca tcacgcacat gcagcaatgc gcgctttatc
     1141 gcaagcggtg cgctgccctc gagcaggctg tcattctccg ccaccgggac ccgccttggc
     1201 gggcccgact gcggtcactt cgccttgtca gccgcggcct tcttgcattc ggagcggaat
     1261 ttcttgcgct ccttgccctt gagccccttg gcgtcggctt ccttcgagca ttcgagcgag
     1321 gccgcggtgc gcgccggttt ctcggccttc ttctccgcag gtgccatttt cggcgccggc
     1381 gccggcgcgg gagtagccgg cgcggtggtt tgggcgaacg ccgagctcac cagcagcatg
     1441 gatgcgaagg cggtcgccgc cacgcgggaa gaaattgtcg tcatcgaaag cacctcgggt
     1501 tgaagatcgg gcaaggtcgc gttgcgatgc tgaaccgcgg atgaataaag cgagctgcgc
     1561 cgccgattcg cgaaacgata ttttagtgca acgagcgatc gctcgttgtc acccgccgtc
     1621 gccgcgctag gatggggacc ttgagattgc catgccccga aggagaccag gatgaccatt
     1681 catgcaaaat tattgtgcgc tgttgcggtg tcgggttttg ccgcactcgc catcacatcg
     1741 ccggcgcagg ccctgaccac gcaggaatgc agtgcaaaat acaaggccgc caaggccgcc
     1801 ggcacgctgg gcgaccagaa gtggaacgat ttccgcaaag cccagtgcgg agcggatgcc
     1861 accgcggcac ccgccgccgc gccggctgcc gcacctggcg ccactgccgc accggcagcg
     1921 ccgaaagccg cggaagccaa ggcgaagaag gaagccgctc ccgccgcggc gccggccgcg
     1981 ccagccgggc ctgcggtgta cccgtccgcc gtcgatccga aatattccaa ggaaagcgcc
     2041 ggcaaggcgc ggatgcacac ctgcgtcgat cagtacaacg ccaacaaggc tgccaacgcc
     2101 aatggcggct tgaagtggat ccagaagggc ggcggctatt acagcgagtg caccaagaag
     2161 ctgaagggct gaagcctgtc gcgacggcgc ggccggcgtt gtggaaatac ggctgcggca
     2221 ataacagcgc ccctcgcaaa gcgcgagggc gctgcacttg cgatgctgaa aggttgacgt
     2281 cgattacttc gacgacagct tgacccagtc gtcatgggcg cgatgtttca gcgcgtccca
     2341 atcgcgggcg gcgacgtccc aattcacgat ggtgcgattg gcctgcgcca cctggccatt
     2401 ggcgaccgtc gatgtctgca tcgagtcgta gatatagacg cctgcaacca gcatcagcgc
     2461 ccccagaatc attcccagaa aaaccggcat ggcaaacctc cgatgatcgc tgacaacgcg
     2521 gtcgggccgg ggatggttcc cttcaggagc gacccgtacc gacgtcatcc tgaggagcgc
     2581 gccctcgcgc gcgtctcgaa ggatggagaa gggcgagatc atccttcgag gctcgccgaa
     2641 gtcggctcgc acctcaggat gacgtacatc atgaacgtgt ctttgttgtc gcaggtccgg
     2701 caaccggtgc tgatgcgggt gccgccgtta gaagcgcatc aatttgtgtg agcaaaaccg
     2761 ctaatgtcac gaccctcacg gggcatcgaa cgcctgcatc agccagtcga tgaagacgcg
     2821 gacgcgcggc gacagctgcc ggttgcgcgg gtagagcagc gataccggcg tcggcgaggg
     2881 tgggcagtcc ggtagtacgg cgacaagggt gcctcgtttg agatcttcct cgacgtgata
     2941 gcgcggcacc tgaatcagtc ccagaccgag gcgcgcggcg gcgacgagac tttcggcgcc
     3001 gttgactgaa accgtcgtgc gcagcatcac ctcgcgcacg gcgccatcga cgacgaactc
     3061 aagcggcagc agagccccgg ttgctgacga atggaagccg accatgatgt catcgtcgag
     3121 ggcatcaacg ctcgcgggca ggccgaagcg atcgatgtaa ccaggtgcgg cgcaggtgat
     3181 ctcctcaagc attgcaacgc ggcgcgcgat catgtcgctg tcctggagat cgccgacgcg
     3241 cagcacgcaa tcgattcctt cgcggatcag atcgaccagc cgatcgcctt cgcccatgta
     3301 gagttcgatg ccgggatatt cggcgagaaa gtccggcagg cgtggcatca caaaatgacg
     3361 cgccagcgtg ccgtgaacat ccacgcgcag cagtcccttc ggcttggcgc cgccgaacgc
     3421 ggcttcggca tcctcgagat cggcgatcag cgacaggcag cgctggtgat aggcctcgcc
     3481 gtccagcgtc gggcggactt gccgcgtggt ccgctccagg agccgcaccc cgaggcgcgc
     3541 ttcgacctgc ttgaccgcgt cggtgaccgt cgagcgcggt aggccgagat cctccgccgc
     3601 cagcgtaaaa ctgcgccggt ccaccacccg cgtgaatacc cgcatcgcgt cgaatcggtc
     3661 catttggatt gttcgtcttt ggcggattgt gatgccggat aatgcctgat tatccgatat
     3721 agcaaaaggc ctagcttcct cccgtcggac gcggaccaca ccgccgaacc atgaagagac
     3781 tgagaatgac caacgaaacc aacaaagtag caatcgtgac gggcgcttcc cgcggcatcg
     3841 gcgccgcggt ggcggagcgg cttgcagggg acggcttcac cgtcgtcatc aattattcca
     3901 gcgatgccaa agccgccgaa acgctcgccc gcaacatcga aagcaagggc ggtcgggcac
     3961 tcaccgccaa agccgacgtc agcgacgcca gcgcggtgcg cggcatgttc gacgcgactg
     4021 aggcggcgtt cggcggcgtt gatgtgcttg tcaacaatgc cggcatcatg aagctggcga
     4081 cgatcgccga cggggacgac gcgctggtcg acagccatat tacgatcaac ctcaagggca
     4141 gcctcaacac gatgcgcgag gcggcccggc ggctgcgcga gggcgggcgc atcatcaatc
     4201 tttcgtcgag cgtggtcggg ctctatcagc cgacctacgg cgtctatgct gcgaccaagg
     4261 ccggcgtcga agcaatgacg catgtgctgt cgaaggagct gcgcgggcgc aacatcaccg
     4321 tcaacgcggt ggcgcctggt ccgacggcga cggcgctctt cctcgacggc aagccgcagg
     4381 cggcgatcga ccatctcgcc aagctcgcgc cgctcgaacg gctcggccag cccgaggaca
     4441 tcgccaacgc cgtcgcattc cttgcagggc ccgacggcgc ctggatcaac ggtcaggtgc
     4501 tgcgcgccaa tggcgggatc atctgacccg cgtccccttc aaccttcaac gcaccgacaa
     4561 cggagattcc atcatgagca agaaaatcat tctcatcacc ggcgcttcga gcggcttcgg
     4621 ccgtctaacg gccgaagcgc tcgccaaggc cggaaacacc gtctacgctt ccatgcgcga
     4681 catcgccggc cgcaacgcca agaacgcggc cgagatggcc gggaccttga agcgcgatgg
     4741 cgtcgatctg cgcgcgatcg agctcgacgt ccaatccgag ccgtcgatca atggggcggt
     4801 caagaggatc atggccgaga gcggcaagat tgacgtgctg gtccacaatg cgggtcatat
     4861 gatgttcggc cccgccgagg cgttcaagcc cgagcagttc gcggagcagt atgacgtcaa
     4921 cgtgctcggc acgcagcgcg tcaaccgggc cgtcctgccg cacatgcgcg cggcgaaaca
     4981 gggcctgctc gtctggatat cgagcagcag ctccgctggc ggaacgccgc cctatctcgc
     5041 gccctatttc gcggccaaag cggcgatgga ctcgctggcc gtcctctatg cgcgcgagct
     5101 gtctcgctgg ggcatcgaga cgacgatcgt cgtgcccggc gccttcacca agggcacgaa
     5161 ccacttcgcg cattccgggc gtccggccga cgaggcacgg gtcgcggaat acgaagcagg
     5221 tccctacaag ggcttcagcg aagaggttct gaaggcgttc gccgcgatcg tacccgacga
     5281 agccgagccc agccgcgtgg cggatgagat cgtcaaggtt gtcgacatgc ccttcggcaa
     5341 gcgcccgttc cgcattcact acgacccgac cggagatggc gccgacgtcg cgttcgccgt
     5401 catcgaccgc gtccgcaacg agatgctgca tcgtgtcgga ttttccgacc tgctcaagcc
     5461 gagggacaac ggctgaagcc tccaagtcag cggcgcgcca cgcgcgccgc tgttgatcgc
     5521 ttgccgcgca gccagtccag catcgctgcg ttcatctcgc ggggatagca atggctgaga
     5581 tcgtcgagct cccggtaggt cacatcggcg cccgccgccg acagcagctg gtgcgtctgc
     5641 cgcgcaactt gcaccggaaa catccagtcg agccggccgt gcacgagata gatcggcaag
     5701 tcccgcaacc tctccgcatc ggccatttcc gcgatcagcg gatggaaggt ggcggcgacg
     5761 ggtgcaagat gggtgaaagg ggaggagctc tcgagcccgg tgacgtagca gaaggtgccg
     5821 ccgtcgctca tcccggtcag cagcagtttt gtgggatcga cgttgcagcg gcttcgcacc
     5881 gaatcgagaa tgcggccgag gttttgggtg tcggcgtctt cacccatcag ggcccaggta
     5941 cttttgttag atgtgttgcc ggttgctgtc ggtgccacca ggatggcgcc gcggcagcgc
     6001 gcgtcgcgca gccagctcca caggaagccg cggccgttgc cgctgccgcc gtgcagcgcc
     6061 atcaccagcg gccaggcgcg gtcgggcgta taatattccg gcacatagag cgagaagccg
     6121 ccgcggctgc cgggctcgtt gtggtcgtgg atgatcccgg tgttctcctt ggcaggtgcg
     6181 gcgagccgcg cctttagctc ggcatcttcg cgcaagcgag gatcgatgaa gaaatcgctt
     6241 accggcggca gttttgcggc aagcggatac agcgcctcct gggcgcgggc ggcgtaacgc
     6301 agcgcgcgaa acacggcaat catgtcaccg ttgccgtgct gtacctcccg caacccggta
     6361 aaagccgcca gcgcggcatc gctggccgct tcgagcgagt tccgtacgct tgcgaatggc
     6421 tccggccaat cggccagccg cgaacggatc gcccgcagct cctgatccgg cgagccggcg
     6481 gcctccatga cggaatcgaa atccggcgga ttgagatggc gcgcgacgaa gccgagcgct
     6541 tccagcgatt gcagcagcgg cggcagcacg gccacgatgt catccaccac ggcctcgctc
     6601 atcgcgcgct ccttccagag catgatccgg aaaagtggat accggttttc cctcgcgaca
     6661 aacgcggaac gcgtttgcgc ggagatcatg ctcaaacaaa aagatagagc ggatgacgat
     6721 tcgaagaaaa gtcatcgcgc tctaatgcag ccgcggcgcc ttgagcagcc tggcgcgatc
     6781 ggtcgaggtc aacaccacgt cgaaggtgac gccctcgtgg aacacggtga gcggcacgtc
     6841 gacgccggca gatccgagag cccagagttt tcggtaaaac gccgtctgac ttgagacttt
     6901 ttcgcctttc accgccagga tgacgtcgcc ggccttgagt tcggcgcgcg ccgccggccc
     6961 cttcgcggag atcccgatca caacgaccct gttgtcgatc tcggtcgtga gcatcccgag
     7021 ccagggccgt gccggcttgt tgacgcggcc gaacttgcgc agatcgtcga gcaccggctt
     7081 gagcagatcg atcggcacga tcatgttgac atgctcggcc ttgccttcgc gctcgcgctc
     7141 gagctgcagc gagccgatgc cgatcaactc gccgcggttc gaaatcaggc cggttccgcc
     7201 ccaattggga tgcgcgggat gggtgaagat cgcctcgtcc agcagatact cccaataacc
     7261 ggcgaattcc tgtttcgccg cgatgtggct ggcgaccgac cgcgtgcgcc cgccggcgcc
     7321 gcccaccacg acgcggtcgc cgattgcggc tgcgctggaa gaaccaagcg gtagcggatc
     7381 gaggccgagc tggcccagcg cctgcaccag gccgaagccg ctcaccgaat cgaagccgag
     7441 cgcgtggcct tccaccacgc ggccgtcgcc aagatgcagc cagacggctt ccgcctcggt
     7501 aatcagatag ccgatcgtca gcaccagccc gtcgtcgatc accacgccat taccggcgcg
     7561 ctcggtaccc aacgtgtcgg cgctgaaggc gtcggggggg atgatcgagt gcaggccgac
     7621 caccgaagaa agcacgcgat cgagatcgaa gctgtagtcg ctcgcgcggg gctgataggc
     7681 cggcggcact ttccattcag tcagagaggg catggaaaat ctcctggcca atcggcgcgc
     7741 cggcgttgcc caattcccgg cggatagcgg cgcacgaaag ccatatcgat actatgtcgc
     7801 aaacgagggt cttgaaagtc tctcgtcatg ggatttgtct ggcgacgaat agaggggtgt
     7861 cgtcaacgaa ataggctggc tggcgtgccg gcaacagggg aggaagcaac aattgtcgca
     7921 cgattccgtt ccttcgcgaa aagggcaacc aggggaaagc ccgttgaaat caactcgccg
     7981 ctcggcctca agcgcgggaa tcctgatgta tcggcgcgtc ggatcgcaac tgcaagtcct
     8041 cctggtgcat ccgggcggac cctggtggcg ccgcaaggac catggctcat ggtcaatccc
     8101 gaaaggtgaa atgaatgcag gcgaggccgc cgctacagcc gcgatcaggg aattcgccga
     8161 ggaaaccggc attgtcctca cgggaccgct tgaaccgctg ggcgagatcc gtcagcgtgc
     8221 cggcaaacgt gtgtttggct tcgcggtcga aggcgacctc gacgttcgga tcgtcaaaag
     8281 caacatcttc gaaatcgagt ggccgccggg aagcggaaag atgcaggcct ttccggaaat
     8341 cgaccgcgcc gaatggttcg atctgcctct cgcccatggc aagatactcg acggtcaacg
     8401 gccctttctc gaccggctcg ccgaacgttc gggccaaccc gggatagacc gcgcatgacc
     8461 gaccccttcg atctgcagcg ctttgtcgat gcgcaagcgc cggtctacca gcgcgtgctc
     8521 gcggaattgc gtcagggcca aaagcaaagt cactggatct ggttcatttt tccgcagctc
     8581 gcaggcctcg gccacagccc gatggcgcag cgatttgcca tcgcctctcg cgaggaagcc
     8641 gtggcctatc tcggccatgg cgtcctggga ccccggcttc gcgagtgcac ggcgcttgtc
     8701 aatgccgttg agggccgaac aatccgggaa atactcggaa gtccggatga cctcaaattc
     8761 tgttcgtcga tgaccctgtt cggcgcggtg tcttcgaatc cggaattcgc cgaggctctc
     8821 cggaaattcc atggcggcgc gccggaccga cggacgctgg acctgcttgc tcgctagagc
     8881 gggatgagtt taggttggag cggcggtgcg ctccctcgcc ccgctcttgc ggggtcgaga
     8941 cgagcgaagc tcgctcttag agggttgggg tgaggggctc tctcacagac tgcgctcgcg
     9001 gatagtcccc cacacccgga tcgcaagagc gatccgacct ctaagtgtct gtccgaagag
     9061 tacttgaatc atatgaatca cctgtgattg aagagtagtg gcctgattcg aggaggggcc
     9121 actgatgtgg acgagcaaga accgcgcccg ttacgaccgc agcaagttgc gatatccg
//
LOCUS       DATNOI010000375         7939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1970017,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000375 DATNOI010000000
VERSION     DATNOI010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970017"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VN968_14950"
     CDS             complement(<1..948)
                     /locus_tag="VN968_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30570.1"
                     /translation="MKKDRQHSVGTQAGLDRALSKRQGRRQFLLKTGGILAAGAFGAV
                     PMRGWAADPVNIGALYPTTGSMAQIGVGCVAAAKLAVEMVNDAGGIKSLGGAKLNLIL
                     SDVQSDTTVTRTETDRLITGNKLSAIHGCFASALTLIASEVAERAKVPLLTGSSSDQL
                     NKGRTYTFTPFARASQFALAQLRMSRLVSDKPKVAVVFENTAFGTSTSNGLREQAAAE
                     GVEIVLFEPYSAGFTDASPLINKVKSSGANALFSVSYLNDLILIVRTVKQVGLNIAIN
                     GGSGGFVIPDFYKNVGKLAEGLQGVAHWNHDMDANAEKVN"
     gene            928..1872
                     /locus_tag="VN968_14955"
     CDS             928..1872
                     /locus_tag="VN968_14955"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXL30571.1"
                     /translation="MLTIFFHAVSPCAIPVQAAVRSLSGMVAPLNIVYNTVSNIIIII
                     ENSCRLWCAGRAERVGRIFQIGAMMVGKPAKHAIARTPKSRQMTISPAREPAAAIIAK
                     SIEEDIVLGRRQPRERLVEQDLCDLFQTHRGDVRLALFELEKKGLVERIPNRGALVRG
                     LTPLEVTEIYAVREELEVMAVRIIPFPVSSNEIERLEELQRQHSAAVAAGDLLTVFYS
                     NLSFHQVLFGLCGNACLIETIDLLARKVYGIRSYANAFPESLDRARQDHLDMIKALRG
                     SRRDDLIVLTRRHLEPSPQAYIKAYERRFGKADLAPAH"
     gene            complement(1929..2996)
                     /locus_tag="VN968_14960"
     CDS             complement(1929..2996)
                     /locus_tag="VN968_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXL30572.1"
                     /translation="MRIAQVAPLTEAVPPKLYGGTERVVHWLTEELVALGNDVTLFAS
                     GDSQTSAKLDATWPKALRLDGSVRDPNALHMVMLERVRQKCDDEEFDFLHFHLDYYPF
                     SLFYRQPTPFLTTLHGRLDLPEHQPVFATFASVPVISISNAQRRPVPQANWVRTIHHG
                     MPEKLLMPRPVTPGYLAVLGRIAPEKGINRAIKIAIRCGIPLKIAAKVDRADQEYYDE
                     IIRPLMDHPLVEYIGEISDHEKSDFLSGAIGLLLPIDWPEPFGLVMIEAMACGTPVVA
                     YNRGSVPEIIDEGLTGFIVEDETSAVRAVDRLSRLSRPAIRKQFEARFTGRRMALDYL
                     AAYRSLKEAAEPRIKLVSSAE"
     gene            3307..5079
                     /locus_tag="VN968_14965"
     CDS             3307..5079
                     /locus_tag="VN968_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30573.1"
                     /translation="MDVLSGYANRPLPFVLRYLRRRPAAHVAILTAVVAAVACSVGTQ
                     YGVKHLVDGLSAGPAHAGSVWLAFVFLMSMIAADNFLWRIASWTASFTFVGVTGDLRR
                     DIFRHLTGHAPSYFSDRLPGMLTSRITATSNAVFTVENMFVWNVLPPCIATVAAIALI
                     GTVSLPMSAGLIVIAGAMVIAMFHLAARGKPLHDEFADKAAAVDGEMVDVINNMPLVR
                     AFCGLRFEHDRFDATVNRELTARGRSLRYLEKLRLTHAAITVALTIALLAWAVVLWQR
                     GLATTGDVVLVCTLGLSILNATRDLAVALVDVTQHVARLTEAIATLLLPHELRDHPEA
                     EPLVKAGAAIVFNKVSFRYPGGLQVFDKFSLRLDAGQRVGLVGPSGGGKSTVFTLLQR
                     FYDVNQGHISIDGQNISRVTQESLREAISVVPQDISLFHRSIMENVRYGRPTATDDEV
                     LRAAIAARCDFVETLPEGLATMVGDRGVKLSGGQRQRIAIARAFLKDAPILLLDEATA
                     ALDSESEEAIREALGRLMRGRTVIAIAHRLATLRNFDRVIMLQAGRIIEDGPPDLLMQ
                     GRGPYRELIAREMDRLATHATHAA"
     gene            5144..7339
                     /locus_tag="VN968_14970"
     CDS             5144..7339
                     /locus_tag="VN968_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HXL30574.1"
                     /translation="MAIEAMIQMIREAEVVSESSFYIPMTGPAARPRRALKHDDTFVV
                     LDSHGDIGASAGGPDGLFNADTRYLARLELVLDGVQPLLLGSNLRDDNSALTVDLTNP
                     DVYRNDRIVLQKDMLHIVRTVFLWRGTAYQRIGVQNHGETTASFDLTLLFDNDFADLF
                     EVRGERRPRRGIGSSKLLGPADVALEYIGLDGSSRSTAVQFDPRPTRLATNAATYHLD
                     LASQQVTALFVSVACNKPAALKPAPFFRGLLAHRREMRRSTSGATSIETSNHIFNEVL
                     CQSMADLNMLTTETPQGRYPYAGIPWYSTTFGRDGLITALQMLWVDPRVAQGVLKRLA
                     FYQAKTVDPLADAEPGKILHEMRGGEMAALREVPFAQYYGSVDATPLFVLLAGLYVER
                     TGDDATLAELWPAIEAALRWIDGAGDPDQDGFVEYQRASEQGLANQGWKDSYDAIFHA
                     DGRLAEGYVALAEVQGYVFAGKHLAARCALRLGLVDKARKLEAEAQRLAERFEDAFWC
                     EELGTYALALDGAKQPCRVRTSNAGQLLFTGIVRHDRARMVAADLMRPHFFTGWGIRT
                     VARGEARYNPMSYHDGSIWPHDNALIALGLARYGLKHSFEQVFKGLFDAASYMDLRRL
                     PELFCGFRRERRRGPTLYPVACAPQAWASATPFTLLEAALGLEFDARRGEIRLRNPRL
                     PTFLNEVFLRDLQLGPSSVDLRVRRHGDDVSLEVLRTRGQIQVSIVLAH"
     gene            7519..>7939
                     /locus_tag="VN968_14975"
     CDS             7519..>7939
                     /locus_tag="VN968_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HXL30575.1"
                     /translation="MRAKRLSMFIAIAVVASTAAISRAADDKGSPQAGRDAPPPATAP
                     AVPSKEAVPPPSVTIIRAADAHGVLGRDVRSAADEDMGRIVDVIVDRGGAVRAAVIDF
                     GGFLGVGSRKIVVDWNALHFGRIANKSDSITLELTKDQ"
BASE COUNT         1418 a         2431 c         2500 g         1590 t
ORIGIN      
        1 attcaccttc tcggcgttgg cgtccatgtc gtggttccag tgcgccacgc cttgcaaacc
       61 ttccgcgagt tttccgacgt tcttgtagaa gtccggaatg acgaagccgc cggatccgcc
      121 gttgatcgcg atgttgaggc cgacctgctt gacggtccgc acgatcagaa tgaggtcgtt
      181 caggtaggac accgagaaca gcgcattggc gccggaagat ttgaccttgt tgatcagcgg
      241 gctggcgtcg gtgaacccgg ctgaatacgg ttcgaacaac acgatctcga cgccttcggc
      301 ggccgcctgt tcgcgcagac cgttggatgt cgaggtgccg aacgcggtgt tctcgaacac
      361 caccgcgact ttcggcttgt cgctgacaag ccgcgacatc cgcaactgcg ccagggcgaa
      421 ttgcgaggcg cgggcgaacg gcgtgaaggt ataggtacgg cccttgttga gttggtcgga
      481 actggatccg gtcagcagcg gcactttcgc gcgttccgcc acctcgcttg cgatcagtgt
      541 cagggcgctg gcgaaacagc cgtggatcgc ggacagtttg ttgccggtga tgaggcggtc
      601 ggtctccgtg cgcgtcaccg tggtgtcgct ctgcacgtcg gagaggatga gattgagttt
      661 cgcgccgccg agcgatttga tgccgccggc gtcgttgacc atctcgaccg cgagcttggc
      721 ggcggcgacg cagcccacgc cgatttgcgc catgcttccg gtagtcgggt aaagcgcgcc
      781 gatattgacc ggatcggccg cccagccgcg catcggcacc gcgccgaacg cgcccgcggc
      841 gagaatgccg cccgtcttca gcaggaattg gcggcggcct tgccgtttcg acaaggctcg
      901 atccaggccg gcttgagtgc cgacggaatg ctgacgatct tttttcatgc cgtttccccc
      961 tgcgcgatcc cggtacaggc tgctgtccgc tcgctttcag gcatggtagc gcccctgaat
     1021 attgtttaca atacagtttc caatattatc atcataattg aaaactcttg ccggctgtgg
     1081 tgcgccggcc gcgccgaacg ggtggggcgc atcttccaaa taggggccat gatggtcgga
     1141 aaaccagcca agcacgcgat cgcgcggacg cccaaatccc gtcagatgac gatatcgcct
     1201 gcgcgagaac cggccgcagc catcatcgcc aagagcatcg aggaggacat cgtacttggg
     1261 cgacgtcagc cgagggagcg cctggtcgag caggaccttt gcgacctgtt ccaaacccat
     1321 cgcggtgacg tgagactggc tctgttcgag ctcgaaaaaa agggcctggt ggagcgcatc
     1381 cccaatcgcg gagcgctggt tcgcggattg accccgctgg aggtgacgga gatctacgcg
     1441 gttcgtgaag agctggaagt gatggcggtc cggatcatcc cgtttccggt cagctcaaat
     1501 gagatcgaga ggctggagga attgcagcga cagcacagcg cagccgttgc ggcaggtgat
     1561 ttgctaaccg tgttttacag caacctcagt tttcaccagg tcttgttcgg cctctgcggc
     1621 aatgcctgtc tgatcgaaac gatcgatctg ctcgcccgga aggtttacgg aattcgctcc
     1681 tatgccaatg catttcctga atcacttgat cgtgcccgcc aggaccattt ggatatgatc
     1741 aaggcgcttc gcgggtcgcg tcgagacgat ctcatcgtgc tcacgcgccg ccatctcgag
     1801 ccttccccgc aagcctatat caaggcctat gaacggcgct ttggcaaagc cgatttagcg
     1861 cccgcgcatt agcggcgtcc gcgccgggga tgtgctgccc ggcgcggacg atgacgatat
     1921 ggaccgagct attcggcgct gctcaccagc ttgatgcgtg gttccgcggc ctccttcagg
     1981 ctgcgatagg cggcgagata gtcgagcgcc atgcggcgcc cggtgaagcg ggcctcaaac
     2041 tgctttcgga tggcaggtct gctcaagcgc gacagccgat caacggcgcg gaccgcactg
     2101 gtttcatcct cgacgataaa gccggtcaag ccctcatcga tgatctcggg gaccgagcca
     2161 cggttatatg caacgacagg cgtcccgcat gccatcgctt cgatcatgac gaggccgaaa
     2221 ggctcgggcc aatcgatcgg cagcagcaac ccaatcgcgc cgctgaggaa gtcggatttt
     2281 tcgtggtcgc tgatctcgcc gatatattcg acgagagggt gatccatcag cgggcgaata
     2341 atttcatcgt aatattcctg gtcggctcga tcgaccttcg cggcgatctt gagcggaata
     2401 ccgcaccgga tcgcaatctt gatcgcacgg tttatgccct tctcgggcgc aatccggccg
     2461 agcacggcca aataaccggg ggtcaccggc cgcggcatca gtaacttttc cggcataccg
     2521 tgatgaatgg tgcgcaccca gttggcctgg ggcaccggac gccgttgggc gttcgaaatc
     2581 gagatcaccg gaaccgaggc gaaagtggcg aacaccggct ggtgttcggg caagtccagc
     2641 ctgccgtgca gggtggtcag gaacggagtt ggttgccggt agaaaagcga gaacgggtag
     2701 tagtcgagat ggaagtgcag gaagtcgaat tcttcatcgt cgcatttctg gcggacgcgt
     2761 tccagcatta ccatgtgcag cgcgttgggg tcgcggacgg acccgtcgag gcgcagcgcc
     2821 ttcggccatg tcgcgtcaag tttcgctgag gtctgggagt cgccgctggc gaacagcgtg
     2881 acatcgtttc ccagcgcgac cagttcctcc gtcagccagt gcacgacccg ttcggtgccg
     2941 ccatagagtt tcggggggac agcctccgtc agcggggcaa cctgcgcgat gcgcatttac
     3001 atctctcctt ttgtgatcct gtgtgtgatc cgggatgacc aaatggtctg tggtcgtgtt
     3061 gacacccgca tacccaaacg ggaacgttct cgcatctgcg aggttcctta gagcaagcct
     3121 acttcttcca catcgcggtc ttgctgatgc catcacgccc aatcagatat ccgcttctgt
     3181 cgatgctgcg cgaatgttgc gcgatgatgg cagtgcgaag ggaacgctgg aacaggctgc
     3241 ctcattcgat cgctcggacc taagtccgcg cgagatttcg ttgtctagca agggggttgc
     3301 agctacatgg acgttctttc aggatacgct aatcgtccgt tgccgttcgt gctgcgctac
     3361 ctccgccggc gccctgctgc gcatgtcgcc attctgaccg ctgttgtagc agcggttgct
     3421 tgttcagtag gcacgcaata tggcgtgaag catctggtcg atgggctgtc ggccgggccg
     3481 gcccacgcag gcagcgtatg gctggcattc gtttttctca tgtcgatgat cgccgccgat
     3541 aattttctgt ggcgcatcgc gagctggacg gcgagcttca catttgtggg agtgacgggt
     3601 gatttgcgcc gcgacatctt ccgtcacctc accggccacg cgccgagcta tttttccgac
     3661 cggctgccgg gaatgctgac aagtcgcatt accgcgacat cgaatgccgt cttcaccgtc
     3721 gaaaacatgt tcgtctggaa cgtgctgccg ccgtgcatcg cgaccgtcgc ggcgatcgcg
     3781 ctgatcggaa ccgtcagcct gcccatgtcg gcgggtctga tcgtcatcgc cggcgccatg
     3841 gtgattgcga tgtttcacct cgccgcgcgc ggcaagccgc tgcatgacga attcgcagac
     3901 aaggccgccg cggtcgatgg cgagatggtc gatgtcatca acaacatgcc gctggtgcgg
     3961 gcgttttgtg gcctccgctt cgagcacgac cggttcgatg caaccgtcaa ccgggaattg
     4021 acggcgcgcg gacgcagcct gcgctatctg gagaagctgc ggctcaccca tgctgcgatt
     4081 accgtggcct tgacgatcgc gttgctggcg tgggctgtcg tactttggca gcgcggcctg
     4141 gccaccacgg gtgacgtcgt gctggtctgc accctcgggc tttcgattct gaatgccacg
     4201 cgcgatcttg cggtggcgct ggtcgacgtt acccagcacg tcgcccgact gaccgaggcg
     4261 attgccacgc tgttgcttcc gcacgaactg cgcgaccatc cggaggccga accgctggtc
     4321 aaggcggggg ccgcgatcgt cttcaacaag gtgtcgttcc gctatcccgg aggcctgcag
     4381 gtattcgaca agttcagcct ccgcctcgac gccggccaaa gggtcgggct ggttggccct
     4441 tccggcggcg gaaaatccac ggtgttcacg ctgctacaga gattttatga cgtgaaccag
     4501 ggccacatct cgatcgatgg ccagaacatc tcccgggtca cccaggagag tcttcgcgag
     4561 gcgatttcgg tggttccgca ggacatctcc ctgttccatc gttcgatcat ggagaatgtc
     4621 cgctacggac ggccaaccgc gaccgacgat gaggtgctcc gcgcggccat tgcggcgcgc
     4681 tgcgacttcg tcgagacatt gccggaggga cttgccacga tggtcggaga tcgcggtgtc
     4741 aagctgtccg gcggacagcg ccagcgcatc gcgatcgccc gcgcattcct gaaggatgcg
     4801 ccgatcctgc tgctggacga agcgaccgcg gcgctcgaca gcgaatccga ggaagcgata
     4861 cgcgaggcgc tcggccgcct gatgcgtggg cgcaccgtca ttgcaatcgc tcaccgtctt
     4921 gcgacgctgc gtaacttcga ccgcgtaatc atgctacaag ctggccgaat catcgaggac
     4981 ggcccgccag atcttctcat gcagggcagg ggtccgtatc gggaactgat agcgcgggaa
     5041 atggaccgtc ttgcaaccca tgcaacccac gcggcctgag cgtctgggat tgttgcaact
     5101 tgctaccgtc gcgcgctgca ggcaaaccgg ctaaggtcac ccgatggcaa tcgaagctat
     5161 gattcaaatg atccgtgagg ccgaagtcgt ctcggaatcg tcattctaca ttccgatgac
     5221 cggacccgcg gcaaggccgc gtcgcgccct caagcatgac gacaccttcg ttgtgctcga
     5281 cagccatggc gacattggcg catccgccgg tggccccgat ggccttttca atgccgacac
     5341 ccgctatctc gcccggcttg aactggtgct ggacggcgtc cagccgctgc tgctcggatc
     5401 gaacctgcgc gatgataact cggcgttgac ggtcgatctc accaatccag atgtctatcg
     5461 caacgaccga atcgtgctgc agaaggacat gctgcacatc gtacgcacgg tctttctgtg
     5521 gcgcgggacc gcctatcaac ggatcggcgt gcagaaccac ggcgagacca ccgcgagctt
     5581 cgatctgacg ctgctgttcg acaatgactt cgccgatttg tttgaagtgc gcggcgagcg
     5641 ccgtccacgg cgcggcatcg gctcgagcaa gctgcttggc cccgccgacg tcgcgctgga
     5701 atacatcggg ttggacggct catcacgcag tacggccgtt cagttcgatc cgcgcccgac
     5761 gcggctggcg accaacgccg ccacctatca tctcgatctg gcgtcgcagc aggttaccgc
     5821 gctgttcgtg tcggttgcct gcaacaagcc ggccgcgctc aagccggcgc cgttctttcg
     5881 cggactgctg gcgcaccgcc gcgagatgcg ccgatcgacg tcaggcgcaa ccagcatcga
     5941 gacctccaac catattttca acgaggtgct gtgccagtcg atggccgacc tcaacatgct
     6001 gacgacggag acgccgcaag gccggtatcc ctatgcaggc atcccctggt attcgacgac
     6061 cttcggccgc gacggcctga ttacggcatt gcagatgctg tgggttgatc cgcgcgtcgc
     6121 gcagggcgtg ctgaagcggc tcgcattcta tcaggccaaa accgtcgatc ctctcgccga
     6181 tgccgagccc ggcaaaatcc tgcatgaaat gcggggcggc gaaatggcgg cgctgcgcga
     6241 ggtgccgttc gcgcaatatt acggcagcgt cgatgcgacg ccgctgttcg tgctgctggc
     6301 cgggctctat gtcgaacgca ccggcgacga tgcgacgctg gccgaattat ggccggcgat
     6361 cgaagcagca ttgcgctgga tcgacggcgc gggcgatccc gatcaggacg gatttgtgga
     6421 atatcagcgc gcctccgaac aaggattggc caaccagggc tggaaggatt cctacgacgc
     6481 gatttttcat gccgacggac ggcttgccga aggctatgtc gcgctcgctg aagtccaggg
     6541 ctatgtgttt gccggcaagc acctggcggc acgctgcgcg ctccgcctgg gcttggtgga
     6601 taaagcgcgc aaactggaag ccgaggcgca gcgtctcgcc gagcggtttg aagacgcgtt
     6661 ctggtgcgag gaactcggca cctatgcgct ggcgctcgac ggcgccaagc agccatgcag
     6721 ggtgcggacg tcgaatgccg ggcagctttt gtttaccggc atcgtgcgcc atgaccgcgc
     6781 gcgcatggtc gccgccgatc tgatgcggcc gcactttttc acgggttggg ggatccgcac
     6841 cgtcgcgcgg ggcgaggcgc gctacaatcc gatgtcctat cacgacgggt cgatctggcc
     6901 gcacgacaat gcgctgatcg cactcggcct cgcgcgctac gggttgaagc attcgttcga
     6961 gcaggtgttc aagggcctgt tcgacgctgc gagctatatg gatttgcggc ggctgcccga
     7021 attgttctgc ggatttcggc gtgaaaggcg ccgcggcccg acgctgtacc cggtcgcttg
     7081 cgcgccgcaa gcctgggcaa gcgcgacgcc gttcaccctg ctggaagcag cgctcggtct
     7141 cgaattcgac gcccggcgtg gcgaaattcg cctgcgcaat ccacgcctgc cgacattcct
     7201 taacgaggtg ttcctgcgcg acctgcaact gggcccctcg agcgtcgatc tgcgggtccg
     7261 tcgccacggc gatgacgtgt cgctggaggt tttgcgtaca cgcggccaga tccaggtgtc
     7321 catcgtcctc gcgcattgat cgcgcgggca ttttatcgtg cgtcggtgag attctgacgg
     7381 gcaaaagata cgaattttga gcgtcgatcc ggattggtcc cgggcgtcat tgggttccac
     7441 ctggaagccg accagctgct tgtgtccggc ctccgcaagt caattcatgc ccttcgatca
     7501 agggcttcag gggtccttat gcgtgcaaaa cgtctctcaa tgttcatcgc gatcgccgtt
     7561 gtcgcgtcga cggccgcaat atcccgggca gccgatgaca aaggttcgcc gcaagcgggt
     7621 cgcgacgccc cgccgccagc caccgccccc gcggtcccgt ccaaggaggc ggtgccgcca
     7681 ccctccgtca ccatcatccg ggcggcggat gcacatggtg tgctcggccg cgatgtccgc
     7741 agcgcggcgg acgaggatat ggggcggatc gtcgatgtta tcgtcgaccg cggtggtgcg
     7801 gtgcgcgcgg ccgtgatcga cttcggcggt tttctcggcg tcggcagccg gaaaatcgtc
     7861 gtcgactgga atgcgctgca tttcggacgc atcgccaaca agagcgacag catcaccctc
     7921 gaactgacca aggatcagg
//
LOCUS       DATNOI010000376         3039 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_195901,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000376 DATNOI010000000
VERSION     DATNOI010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195901"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VN968_14980"
     CDS             complement(<1..1472)
                     /locus_tag="VN968_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HXL30576.1"
                     /translation="MSSYSEDLHSAALAYHRFPKPGKLEIQAIKPLANQRDLALAYSP
                     GVAAACTAIAENPAEAASLTTRANLVAVVSNGTAVLGLGNIGPLASKPVMEGKAVLFK
                     KFAGIDVFDIEIAADTIDRVVETVAALEPTFGGINLEDIKGPECFEIEAQLKARMKIP
                     VFHDDQHGTAIIVAAAITNALLLNGKNLKDVKIVASGAGAAAIACLNLLVSMGAQRKN
                     IWVVDIDGVVHEGRTTLMDRWKSVYAQKTDKRALADVIGGADIFLGLSAPNVLTPDMV
                     KQMADKPLVMALANPTPEIMPDEARKARPDAMICTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATAINEEMKKAAVDAIAQLARDPPVDASAAGFDSGEAQGFGPGSLIPSPFDLRLI
                     LRIAPAVARAAMASGVASRPIANFDDYAAQLERFAFRSGLVMKPVFAKAKTQPVRVIY
                     AEGEDERVLHATQVVLGEKLARPILVGRPSVVEARIKRFGL"
     gene            complement(1527..>3039)
                     /gene="aspS"
                     /locus_tag="VN968_14985"
     CDS             complement(1527..>3039)
                     /gene="aspS"
                     /locus_tag="VN968_14985"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HXL30577.1"
                     /translation="ELPTGEVELYVSEIEVLGPAGELPLPVFGEQEYPEDIRLKYRFL
                     DLLREKLHQNIMTRGAIVDAMRKRMKDQGFFEFQTPILTASSPEGARDFLVPSRIHPG
                     RFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVEMSFVTQD
                     DVFTALEPVITGVFEDFAKGKPVTKNWPRIPFAKALRYYGTDKPDLRNPIVMQDVSEH
                     FRGSGFKVFARMLEDSRNQVWAIPAPGGGSRAFCDRMNSWAQGEGQPGLGYIMWREGG
                     EGAGPLANNIGPERTAAICGALGLKEADAAFFVAGDPDKFWKFSGLARTKLGEELNLI
                     DKDRFELAWIVDFPMYEYNEEEKKIDFSHNPFSMPQGGLEALQTQDPLTIKAFQYDIT
                     CNGYEIASGGIRNHRPEAMVKAFEIAGYGEKDVVERFGGMYRAFQYGAPPHGGMAAGV
                     DRIVMLLCGTTNLREISLFPMNQRAEDLLMGAPSEVTPKQLRELHIRLNLPQT"
BASE COUNT          567 a          962 c          930 g          580 t
ORIGIN      
        1 aggccgaacc gcttgattcg cgcttcgacc accgagggac gtccgaccag tatcggccgc
       61 gccaacttct ccccgagcac cacttgcgtc gcgtgcagga cgcgttcgtc ctcgccttcc
      121 gcatagatca cccgtaccgg ctgggtcttc gccttggcga ataccggctt catgaccagg
      181 ccggagcgga acgcgaagcg ctccagctgc gccgcatagt cgtcgaaatt tgcgatcgga
      241 cgggacgcga caccggatgc cattgccgcc ctcgcaaccg cgggcgcaat gcgcaggatc
      301 agccggagat caaacgggct cggaatcagc gaacccgggc cgaagccttg cgcctcgccg
      361 ctgtcgaagc cggccgcgga cgcatccacc ggtggatcgc gcgcgagctg tgcgatcgcg
      421 tcgactgcgg ccttcttcat ctcctcattg atcgcggtgg cgccgacatc cagcgcgccg
      481 cgaaagatga agggaaagca caggacgttg ttgacctgat tgggaaagtc ggaacggccg
      541 gtgcagatca tcgcgtcggg gcgcgccttg cgggcttcgt ccggcatgat ttccggcgtc
      601 ggattggcca gcgccatcac caatggcttg tccgccatct gcttgaccat gtcgggtgtc
      661 agcacattcg gcgccgacag accgaggaaa atgtcggcgc cgccgatcac gtccgccagc
      721 gcgcgcttgt cggtcttctg cgcatagacg gacttccagc ggtccatcag cgtggtgcgg
      781 ccctcatgca ccacgccatc gatatcgacg acccagatgt tcttgcgctg cgcgcccatc
      841 gagaccagca gattgagaca cgctatcgcc gcggctccgg cgccggaggc gacgatcttg
      901 acgtctttaa gattcttgcc attcagcaac agcgcattgg tgatcgcggc ggcgacgatg
      961 atggcggtgc catgctgatc gtcgtggaac accgggatct tcatgcgcgc cttgagctgc
     1021 gcctcgatct cgaagcattc cgggcccttg atgtcctcga ggttgatgcc gccgaatgtc
     1081 ggctccagcg cggccacggt ctcgaccacg cggtcgatgg tatcggcggc gatttcgatg
     1141 tcgaacacgt cgatgccggc gaacttcttg aacagaaccg ccttgccctc catgaccggt
     1201 ttcgatgcca gcggaccgat attgccaagg ccgagcacgg cggtgccatt ggagacgacc
     1261 gcgacaagat tggcgcgggt ggtcagcgat gccgcttccg ccggattttc ggcaatcgcg
     1321 gtgcaggcgg cagccacccc cggcgaatag gccagcgcaa gatcgcgctg gttggcgagc
     1381 ggcttgatcg cctgaatctc aagcttaccc ggcttcggaa accggtgata agcaagcgcc
     1441 gccgaatgga ggtcttcaga ataggacgac atactttccc cccgcgggct cctcgacccc
     1501 gaattcaagg cttcggtggc tggtctttag gtttgcggca gattgagccg gatgtgaagc
     1561 tcgcgcaact gcttcggcgt gacctccgaa ggcgcgccca tcagcaggtc ttcggcgcgt
     1621 tggttcatcg gaaacagcga gatttcgcgc aggttcgtcg tgccgcacag gagcatgacg
     1681 atgcgatcga ccccggcggc catgccgcca tgtggcgggg caccatattg gaaggcgcgg
     1741 tacatgccgc cgaagcgctc gaccacgtcc ttctcgccat aaccggcgat ctcgaacgcc
     1801 ttcaccatcg cctcgggtcg gtgattgcgg ataccgccgg aagcaatctc gtagccgttg
     1861 caggtgatgt cgtattgaaa cgccttgatg gtcagcggat cctgagtttg caacgcttca
     1921 aggccgccct ggggcatcga aaacggattg tgcgagaaat cgatcttctt ctcttcctcg
     1981 ttgtattcgt acatcgggaa gtcgacgatc caggccagtt cgaagcgatc cttgtcaatc
     2041 aggttcaact cttcgccaag cttggtgcgt gcgaggccgg aaaacttcca aaacttgtcg
     2101 ggatcgccgg cgacaaagaa cgcggcatcg gcttccttca accccaaggc accgcatatg
     2161 gccgccgtcc gttccgggcc gatgttattg gcgagcggac ctgcgccctc gccgccctcg
     2221 cgccacatga tgtaaccgag cccgggctgg ccctcgccct gcgcccagga attcatgcgg
     2281 tcgcagaacg cgcgcgagcc gccgccaggt gcggggatcg cccagacctg gttcctggaa
     2341 tcctcgagca ttcgcgcgaa caccttgaag ccggagccgc ggaaatgctc cgagacgtcc
     2401 tgcatgacaa tcggattgcg caagtccggc ttgtcggtcc cataatagcg caaggctttc
     2461 gcgaacggaa tccgtggcca gtttttggtc acgggcttgc cttttgcaaa gtcctcgaac
     2521 acgcctgtga tgaccggctc cagcgcggtg aacacatcgt cctgcgtgac aaaactcatc
     2581 tcgacgtcga gctggtagaa ctcgcccggt aaccggtcgg cgcgcgggtc ttcatcgcgg
     2641 aaacagggcg cgatctggaa atagcggtcg aagcccgaca tcatcaggag ttgcttgtac
     2701 tgctgcggcg cctggggcaa cgcgtagaac ctgcccgggt ggatccgcga cggcaccagg
     2761 aagtcgcgcg cgccctccgg cgaggacgcc gtcaggatcg gggtctggaa ctcgaagaaa
     2821 ccctgatcct tcatgcgctt gcgcatggca tcgacgatcg cgccgcgagt catgatgttt
     2881 tgatgcagtt tctcgcgaag aaggtcgagg aaacggtact tgagccttat gtcttcagga
     2941 tattcctgct caccgaacac cggcagcggc agttcaccgg ctggccccag cacctcgatt
     3001 tcactaacat agagctcgac ctcgccggtc ggcaattcg
//
LOCUS       DATNOI010000377        11022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_935248,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000377 DATNOI010000000
VERSION     DATNOI010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11022
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935248"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2748
                     /locus_tag="VN968_14990"
     CDS             <1..2748
                     /locus_tag="VN968_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXL30578.1"
                     /translation="LNSDSLNEQELYDFGIYRVRQQLAPVSGVTLPTPAGGKYRQIMV
                     DIDPDKLLSRGLTPLDIVNAVNTQNLTLPSGTAKIGDTQYVVRTNATPASIDDLNMMP
                     VKFANGATIFLKDVAQVRDGSLVQQNIVRENGRRSVLLSVIKNGNASTLAVVNGVKNA
                     LAAIRAAAPPGLKIDELFDQSVFVTNSVNGVLREGAIAAGLTALMILVFLGSWRSTLV
                     VMISIPLAILSSLVVLYFLGETLNTMTLGGLALAVGILVDDSTVTIENTHRLWTEEGM
                     PLSEATLHGAAEIAVPTLVSTLAISCVFTSVVFLDGPAKYLFTPLGLAVVFAMLASYG
                     LSRTLTPITIGLLLKSERHGADHGTPAGVFSRASAAFDRGFERLRDGHSRLLRMLLQR
                     RTIVPVVAVLMLSLGGAMFHFVGRDFFPLIDGGQIQLHVRAPAGTRIESTEAVFQAIE
                     DKIREVIPEQERSLIVDNIGLPARAYNLAFSDGSTIGVNDGVIQVALKEGHKPTADYV
                     KKLRQVLPAAFPEVVFYFQAADIVTQILNFGLPAQIDVRTVGYDLNNLSVAKELRQRL
                     AEIPGIVDAHLQQEVDAPAFYAQIDRTRAAQLGLNASTVATNINVSLSSSEQVSPNFW
                     TDPTSGIPYYLAVQTPEYKVSSLNALGNTPVSSSLSVSGQTVPGMLSNVATFKRDTLP
                     TNANQSNIQPVFDVYASVQGRDLGSVAAAISKVTTELQKKLQPGNSIQVVGQIQSMND
                     SFRNLGIGLLFAAVFVYLLMVVNYQTFGDPFVVILALPATLCGIVTMLFITGTTLNVP
                     SLMGAIMAVGVASANSILLVTFAREQQLKGHSAFEAALSAGHTRIRPVLMTAAAMIVG
                     MIPMAIGGAGEEQNAALARAVIGGLLFATPTTLLIVPYLFAMLRKGNDGKPHHGVFEE
                     V"
     gene            2751..4025
                     /locus_tag="VN968_14995"
     CDS             2751..4025
                     /locus_tag="VN968_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXL30579.1"
                     /translation="MTEINVRQKNEGVQDRIDPDADRVIALPNVARERRRYAGALLGA
                     GALLLLLGGLGLGGWRHYQTALDLAVAAQQSRTLVPEVRVAAVRASDSKITVSLPATT
                     TAFEAANIFARTSGYIEKRYVDIGDRVKAGALLADIIAPELDHQITQARATLAQNQAT
                     LQQTQASRELAQVTNARDSNLVKQGWLTLQQGDNDRLTLQAQQAAVAVAQSNIEAQQA
                     QIRILEQEKAYQRVVAPFDGVITQRAIDNGSLVQSGSTFMFTLMHPDVIRTQVFVPQD
                     QAFGLGPGVDAVVRVPEIPDRTFPGKVTRIASALQPGSRTLLTEIDVPNPDGALSPGI
                     YCTVELYIPRKTPSMIIPADAVVFDQNGLHVAVVENGIIHLQKIAIARDFGTQVEVHD
                     GVKPGDQVVLNPMVNLAEGSKVAVRKNKPQIS"
     gene            4048..4989
                     /locus_tag="VN968_15000"
     CDS             4048..4989
                     /locus_tag="VN968_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30580.1"
                     /translation="MRRLLFTSTAILLAGCSACLAQVSTMGSTAMGLPSTPGTIVTSP
                     LNGPSPFSATTQPGTADTTLAPIPLASDPTTPGTVVTCSTPTGQIGPGTPTVTVTSMS
                     TGTLPSIPAAPTMSAAPDNTTAPILALSAQPATSFMSTIPGSTAPAQTPAIPAPSPPS
                     INAAPIATSIASSTATLTPSTMTLSTTMMPTTPLGTIPTPFVSSTTGTISPASPLGSA
                     SATVCSSAPGGPPTNGAAMPLSTSDIPSTPPPGTIQPAVTQLAGTSIDPTMAVMPTPN
                     TPACAESVTMNLATPGMMAPANATGAAATPGVSPAGC"
     gene            5426..6103
                     /locus_tag="VN968_15005"
     CDS             5426..6103
                     /locus_tag="VN968_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL30581.1"
                     /translation="MAKVLLIEDDSETAEEITAELADRGFEVEWSANGIEGLDKARSS
                     RPDAMIVDRLLPGMDGLTVIEALRKEQVRTPVLVLSALGAVDDRVRGLRMGGDDYLTK
                     PFAVVELVARIEALLRRPAESRETILRVGPLELDLIERTAKRGDRAIDLLPREFRLLE
                     YMMQRSDQLLTRAMLLEEVWNYKFVPATNLVDVHMGRLRHKVDGPDDAPMIHNVRGAG
                     FILRAGS"
     gene            6174..7538
                     /locus_tag="VN968_15010"
     CDS             6174..7538
                     /locus_tag="VN968_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL30582.1"
                     /translation="MRQPQFMRSNTFHWAFMVGGVFAVFIIVLFGFIYWKIDDYLTAR
                     SDRVITAQLEGIAALPPERRLEAIDERLRQDPRGVQLAAIFAADGHRMTGNLESLPPD
                     LRIDAEAQGADIIRTDRNGREHQTVRAIARRMRNGDVLVLGRNVDEAKEISHVVGQAL
                     ALGLLPGFSLCLLAGAWLSVRAQKRVEEVNQRVQRIIAGDLRERLPHRSVDEPFSKLA
                     VIVNGMLDEMETMIHALAGVGNDIAHDLRTPLTRARLTLERGRTNAATLEQLQVVADK
                     AIAGIDQSLAIITALLRLAEIENSRRSAGFGTVALDEMLREVCDIYEPIAENKNIALR
                     VGATQPLTVRGDRDLLIEAVANLVDNAIKFTPEGGKVDIALLRIEDETIVRVTDTGCG
                     ISEQEREAVLRRFYRSDKMRNTPGVGLGLNLVAAIVKLHGFRMAIRSGPGCRVEIVCP
                     DRQD"
     gene            7867..8139
                     /locus_tag="VN968_15015"
     CDS             7867..8139
                     /locus_tag="VN968_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30583.1"
                     /translation="MSSRPLTAVVFVGAVLFGSAMADADPLPSPVTSPVTTVPQAPIG
                     HLQPHAHPISPSAPAEEIEQQRLSTFDAEQQKLDQQLDKSLNICRC"
     gene            8314..8405
                     /locus_tag="VN968_15020"
                     /pseudo
     CDS             8314..8405
                     /locus_tag="VN968_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313921.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
     gene            8901..9200
                     /locus_tag="VN968_15025"
     CDS             8901..9200
                     /locus_tag="VN968_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXL30584.1"
                     /translation="MAVQMTKSQLIEKIAVENEVGKKDVKGVLETLATIGYKELKKNG
                     VFLVPGFAKFVVIKKAATKARKGVNPFNGEPMTFKAKPARKIVRARPVKAAKDAV"
     gene            9656..10684
                     /locus_tag="VN968_15030"
     CDS             9656..10684
                     /locus_tag="VN968_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXL30585.1"
                     /translation="MLDVTRDPSGDGIPPQAAESASANDAGLLDAYSNAVIDVTERVG
                     PAVVRVETGSGLPGARERGGLGSGIVISPDGLVLTNSHVVGSSRQIRLRDNEGIVTDA
                     RVLGVDPDTDLALLRADGARDLRYASLGNSKTLRRGQLVVAIGNPLGFESTVTAGVVS
                     ALGRSIRSVSGRTIEDVIQTDAALNPGNSGGPLVSSAAEVIGINTAIISGAQGICFAV
                     ASNTAQFVLSEIIRHGYVRRAYIGVSGQTAPIPRRHAVVAGVDNKMGALLAQIEPDGP
                     AARAGLLPGDVVIRLDGVEVNGVDDLIRALDRDRIDRTLEMDVLRMGRLRTVEIHPVE
                     RKPAAARK"
     gene            complement(10752..>11022)
                     /locus_tag="VN968_15035"
     CDS             complement(10752..>11022)
                     /locus_tag="VN968_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30586.1"
                     /translation="LLIFRTQGAVPVFVDGRAATAYPDDLLRDYFKLVELEISETAWD
                     TVLEKYQIDTVLWVKAHEQLRRFLVDKRGWKEDYAGMYESIYVKP"
BASE COUNT         2126 a         3444 c         3399 g         2053 t
ORIGIN      
        1 ctcaattcgg atagcctgaa cgagcaggag ctctacgact tcggaattta ccgcgtccgg
       61 caacaactgg cgccggtttc cggcgtcacg ctgccgacgc cggcgggcgg caaatatcgt
      121 cagatcatgg tggacatcga cccggacaag cttctgtcgc ggggactgac gccgctcgac
      181 atcgtcaatg cggtcaatac ccagaacctg accctgccgt cgggaacggc gaaaatcggc
      241 gacacccaat atgtggtgcg gaccaacgcc acgccggcat cgatcgacga tctcaacatg
      301 atgccggtca agttcgccaa tggcgccact atcttcctga aagatgtggc gcaggtacgc
      361 gacggatcgc tggtgcagca gaacatcgtg cgcgagaatg gccgccgctc ggtcttgctc
      421 agcgtgatca agaacggcaa tgcctcgacg ctggcagtcg tcaacggcgt gaagaatgcc
      481 ctggcggcga tccgcgccgc cgcgccaccc ggactcaaga tcgacgaact gttcgaccag
      541 tcggtattcg tcacgaactc ggtcaatggc gtgctgcgtg aaggcgccat cgcggcaggc
      601 ctcaccgcgt tgatgatcct ggtcttcctt ggttcctggc gatcgacgct ggtggtgatg
      661 atttcaatcc cgctggcgat cctgtcgtcg ctcgtcgtcc tgtactttct cggcgaaacg
      721 ctgaacacca tgacgctggg cggtctcgcg ctggcggtcg gcatattggt cgacgattcg
      781 accgtgacca tcgagaacac tcaccgcctg tggaccgaag agggcatgcc gctctccgag
      841 gcgacgttgc acggcgccgc cgaaatcgcg gtgcctaccc tggtttcgac gctcgccatc
      901 agctgcgtgt tcacgtcagt tgtgttcctc gacggtccgg caaaatatct gttcacgccg
      961 ttgggtctgg ccgtggtgtt tgcgatgctg gcgtcctacg gactgtcgcg aaccctgacg
     1021 ccgatcacga tcggcttgct gctcaagagc gaacgtcatg gcgcggacca cgggaccccg
     1081 gcgggcgtct tctcgcgggc ttccgccgcg tttgaccgcg gcttcgagcg cctgcgtgac
     1141 ggacattcga gattgctcag gatgctgctt caacggcgaa cgatcgtgcc tgtcgtcgcg
     1201 gtcctgatgc tcagcctcgg cggggccatg ttccattttg ttggccgcga cttcttcccg
     1261 ctcatcgacg gtggccagat tcagcttcac gtccgcgccc ccgccggcac ccgcatcgag
     1321 agcaccgagg cggtattcca ggcgatcgag gacaagattc gcgaggtgat tccggagcag
     1381 gagcgctcgc tgatcgtcga caatatcgga ctgccggcgc gtgcctataa cctggcattc
     1441 tccgacggct cgacgatcgg ggtcaatgat ggtgtgatcc aggtggcgct caaggagggc
     1501 cacaagccga ccgccgatta cgtcaagaaa ttgcgtcagg tgttgccggc agcgttcccg
     1561 gaggtcgtgt tctacttcca ggcggccgat attgtaaccc agattctgaa cttcggactg
     1621 ccggcgcaga tcgacgtccg gacggtgggc tatgacctga acaatctttc ggttgcaaag
     1681 gagctccgcc aacgcctcgc tgagattccc ggcattgtcg atgcgcatct gcagcaggaa
     1741 gtcgacgcgc cggcgttcta cgcccagatc gaccggaccc gggccgcgca gcttggcctc
     1801 aatgccagca cggtggccac caatatcaat gtcagcctca gctcttcgga gcaggtgtca
     1861 cccaacttct ggaccgatcc gacatcggga attccgtatt atctggcggt gcaaacaccg
     1921 gaatacaaag tcagctcgct caacgcactt ggaaacacgc cggtttccag ttcgctgtcg
     1981 gtgagcggcc agacggtccc cggcatgctc agcaatgtcg cgaccttcaa gcgcgacacg
     2041 ttgcccacca acgctaacca atccaacatc cagccggttt tcgacgtcta tgccagtgtg
     2101 cagggccgcg atctcggcag cgtcgcagca gccatcagca aagtcacaac cgagctgcag
     2161 aagaagctgc aacccggcaa ctcgattcag gtggtcggcc agatccagag catgaacgac
     2221 tccttccgga acctcggcat cgggctgctg ttcgccgcgg tcttcgtcta tctgctgatg
     2281 gtcgtcaact accagacctt tggcgatccg ttcgtcgtga tcctggcact gccggcaacc
     2341 ctctgcggca tcgtcacgat gctgttcatc accggtacca cgctgaacgt gccgtcgctg
     2401 atgggagcga tcatggcggt cggcgtcgct tcggcgaact cgatcctgct cgtgaccttc
     2461 gcgcgcgagc aacagctcaa gggccactcc gccttcgagg ccgcgctgag tgccggccac
     2521 acccgcatcc ggccagtgct gatgaccgcc gccgcgatga tcgtcggcat gatcccgatg
     2581 gcgatcggcg gcgccggcga ggaacagaac gcagcacttg cgcgcgccgt catcggcggc
     2641 ctgctgttcg cgacgccgac aaccctgctg atcgtgcctt acctgttcgc gatgctgcgc
     2701 aaaggtaatg acggaaagcc ccaccatggc gtattcgagg aagtctagga atgaccgaga
     2761 ttaacgtgcg gcagaaaaat gaaggtgttc aggaccggat cgatcccgat gccgatcgcg
     2821 tcattgcgct gcccaatgtg gcgcgcgagc ggcggcgtta cgcaggcgcg ctgctcggcg
     2881 caggcgcgct gctgttgctg ctgggcggcc ttggattggg aggctggcgc cactaccaga
     2941 ccgcactcga tctcgccgtc gccgcgcaac agagccggac gctggttccc gaggttcgcg
     3001 tcgccgcggt ccgcgccagc gacagcaaga ttaccgtcag tttgccggcg accacgacgg
     3061 ccttcgaggc ggcgaatatt tttgcgcgta ccagcggcta catcgaaaag cgctatgtcg
     3121 acatcggtga tcgggtcaag gcaggagccc tgctggcgga catcatcgcc ccggagctcg
     3181 accaccagat tacgcaggcc agggcgacgc tggcccagaa ccaggcgaca ttgcagcaga
     3241 cgcaggcaag ccgtgagctc gcccaggtca ccaatgcgcg cgacagcaat ctggtcaaac
     3301 aaggatggct cacgcttcag cagggcgaca atgatcggct gaccctgcag gcgcagcagg
     3361 cggcggtggc agtcgctcag tcgaatatcg aggcccagca ggcccagatt cgaatattgg
     3421 aacaagaaaa ggcttaccag cgcgtcgtgg cgccattcga cggcgtgatc acgcagcgcg
     3481 ccatcgacaa cggcagtctg gtgcagtccg gatcgacctt catgttcacg ctgatgcacc
     3541 ctgacgtgat ccgcacccag gtgttcgtgc cccaggatca ggccttcgga cttgggcccg
     3601 gcgtcgacgc ggtggtccgg gtccccgaga ttccggatcg aacctttccc ggcaaggtca
     3661 cgcgtatagc gagcgcgttg cagcccggca gccggacgct tctgaccgag atcgatgttc
     3721 ccaatccgga cggcgcgctc agtcccggga tctattgtac cgtcgaactg tatatcccga
     3781 gaaagacacc gtcgatgatc atccccgccg acgctgtggt cttcgatcag aacggcctgc
     3841 atgtcgcggt cgtggagaac ggcatcatcc atctgcagaa gatcgcgatt gcgcgggact
     3901 tcggcacaca ggtcgaagtg cacgacggcg tcaagcccgg cgaccaggtg gtcctcaatc
     3961 cgatggtgaa cctggcggaa ggcagcaagg tcgcggttcg caagaacaaa ccgcagatca
     4021 gctagagcag cggaggaggg attggcgatg cgacgcttgc tgttcacatc aacggccatc
     4081 ttgctggcag gctgcagcgc ctgtctggcg caagtctcca ccatgggcag caccgccatg
     4141 ggccttccat cgacgccagg gacgatcgtc acatccccgc tcaatggtcc gagtccgttc
     4201 tcggcgacaa cccagcccgg aactgccgac acgacgctgg cgccgatacc gctggcgtct
     4261 gacccgacaa cgccggggac ggtcgtgacc tgctccacgc cgacggggca gatcgggcct
     4321 ggaacgccga cggtaacggt cacctccatg tcgaccggaa cgctgccatc gattcccgcg
     4381 gcgccgacga tgtcagcagc accggacaat acgactgcgc caattctggc gctgtctgcg
     4441 caaccggcta catcattcat gtccacaatt cccggctcga ccgcaccggc gcagacgccg
     4501 gcaataccgg ctccatctcc gccctccatc aacgcagcgc ccatcgccac atcgatagcg
     4561 agcagcacag ccacgttgac gccatcgacc atgacgctat cgaccacaat gatgccgacg
     4621 acccccttgg ggactattcc cacgccattc gtcagcagca cgaccgggac catatcgcca
     4681 gcgtcgccgc tcggcagcgc atcggcgacc gtatgcagct ctgcgccggg ggggccgccg
     4741 accaatggtg ccgctatgcc gctctcgacc tctgatattc cgagcacccc tccgcccggc
     4801 acgatccaac ccgcggtcac gcagcttgca ggcaccagca tcgatccgac aatggccgtg
     4861 atgccgacgc caaacacgcc ggcatgcgcc gagagcgtga cgatgaatct ggcgacaccc
     4921 ggcatgatgg cgccggccaa cgcgacggga gccgccgcaa cacccggcgt atcgcccgcg
     4981 ggttgctgac ggcaacagcg ccgcctcacg cgttttccgg aatgcgtcgg atgcgtccgg
     5041 gcatccacca aacttgctcc cggccggtcc cgagaattca cctcgcttat cgcggctgac
     5101 cgagggccaa tactaaaacc ctatttaatg gcaggaccga tttttcgagc ctatgttttt
     5161 ctcgagcggc cccaccgcgc ttgcccccgg aggcggtggt cagatggccg cagagcggac
     5221 ggcgcacgcc ccgtcctatg cccccgaatg agacgggcgg cgctgtccgg ctctttaact
     5281 aacggcgaaa ccgattcaag gacggcgttc ttggcatttg caggtcgttg acattatggc
     5341 attggtggcg ggcagcaagc gtacggttgc agggatgtca ggtttgggcg ccgcgaatgc
     5401 gttcgcggca ggtgggaatc agttgatggc gaaagtgctt ctgatcgagg atgacagcga
     5461 aacggctgaa gaaattacag ccgagctcgc cgatcgcggt ttcgaagtcg aatggtcggc
     5521 caacggcatc gaaggcctcg acaaggcacg gtcgtcgcgg cccgacgcca tgatcgtcga
     5581 tcggctgttg cccggaatgg acggcctcac ggtgatcgaa gcgctgcgga aggagcaggt
     5641 gcgcacaccg gtgctggtgc tgagcgcgct cggcgcggtg gacgatcgcg tacgcggatt
     5701 gcggatgggt ggcgacgact atctcaccaa gcccttcgcg gtcgtcgaac tcgtcgccag
     5761 aatagaagca ttgcttcgcc ggccggcgga atcgcgcgag acgatattgc gggtagggcc
     5821 gctggaactc gatctgatcg agcgcaccgc caaacgcggc gatcgcgcga tcgatctgct
     5881 gccgcgcgag ttccgcctgc ttgaatacat gatgcagcgc agcgaccaac tattgacgcg
     5941 ggccatgctg ctcgaggaag tctggaatta caaattcgta cccgcaacca accttgtcga
     6001 cgtgcatatg ggccggctgc gtcacaaggt ggacggaccc gacgacgccc cgatgatcca
     6061 caacgtccgc ggtgccgggt tcattcttcg cgccggatct taaactttca gatggccgcg
     6121 gcggcgatgc gccgctgttg ccgtcgagcc agatatcggg gcggcgaaat ccgatgcgtc
     6181 aaccgcagtt catgcgttcg aacacgttcc actgggcgtt catggtcggc ggcgtgtttg
     6241 cggttttcat catcgttctg ttcggattca tctactggaa aatcgatgac tatctgaccg
     6301 cgcggtccga ccgtgtgata acggcacaac tggaggggat cgccgcgctg ccgcctgaac
     6361 ggcggctcga ggccatcgac gagcggctgc ggcaggatcc ccggggcgtg caactggcgg
     6421 cgatctttgc cgctgacggt caccggatga ccggcaatct ggagagcctg ccgcccgatc
     6481 ttcggatcga tgccgaagcc caaggcgccg acatcatcag gaccgatcgc aacggccgcg
     6541 agcaccaaac tgtccgtgcg atcgcgcggc gcatgcgaaa cggcgacgtg ctggtgctgg
     6601 gacgaaatgt cgacgaggcc aaggagatct ctcacgtcgt cggccaggca ctcgcgctgg
     6661 gcctgttgcc gggctttagt ctttgcctgc tggccggcgc gtggctgagc gtgcgcgcgc
     6721 agaagcgggt cgaagaggtc aaccagcggg ttcagcgcat catcgccggt gatctgcgcg
     6781 agcggcttcc gcaccgtagc gtcgatgagc cgttctcgaa actcgcggtc atcgtcaatg
     6841 gcatgctcga tgaaatggaa accatgatcc acgcgctggc cggagtcggc aacgatatcg
     6901 ctcacgacct gcgaacgccg ctgacgcggg cgcggcttac gcttgagcgc ggccgcacca
     6961 acgcagccac tctggagcag ctgcaggtgg tcgcggacaa ggcgatcgcg ggcatcgatc
     7021 agtcgctggc catcatcact gcgctattgc gtcttgcgga gatcgaaaac agccggcggt
     7081 cggcgggctt cggcaccgtc gcgctggacg agatgctgcg cgaggtctgc gatatctacg
     7141 agccgatcgc cgaaaacaag aatatcgccc tgcgtgtcgg agcgacgcaa cctctcacgg
     7201 tgcgcggcga ccgcgacttg ctgatcgagg cggtcgccaa cctggtcgac aacgccatca
     7261 agttcacgcc tgaaggcggc aaggtggaca ttgcgttgtt gcgcatcgag gacgaaacca
     7321 tcgtgcgcgt gacggatacc ggatgcggca tcagcgaaca ggagcgcgag gctgtgctgc
     7381 ggcgttttta ccgttccgac aagatgcgaa acacgccggg cgtgggcctt ggcctgaatt
     7441 tggtcgctgc gatcgtaaag ctgcacggct tccggatggc gatccgctcc gggccgggct
     7501 gccgggtgga gattgtctgt cccgaccggc aggattgatg gcaagtcgga tggaaagacc
     7561 acggcacgga cgcgcgggct gttttgccga gcagatgcgt cagtcgtgcg gagttcgaaa
     7621 ttcaaagcca cgatgacgga cgcgttgcga tccggagtgt cgtcgatgca atcggtgcgt
     7681 tgcgctcatg ctgttgcgtc gctcgcgatc atttcaacga ggcgtgatca acgaacccga
     7741 caatgaacgc gtatttaatg ggaatgcggg ctttccccgc ttatcttttt gtctggtcca
     7801 attcatgaag cgcactcaag gccggcgcct gatgtatggc cgagcgcaca accgatggag
     7861 cagccgatgt cctcacgtcc tctaaccgca gttgtcttcg tcggtgctgt cttgttcggc
     7921 agcgcaatgg ccgatgccga cccgctccca agtcctgtaa caagtccggt aacaacggtg
     7981 ccacaagcgc cgatcggcca tctgcagccg cacgcgcatc cgatttctcc gagcgcccct
     8041 gcggaggaga ttgaacaaca gcggttgtcg acattcgatg ccgagcagca gaagcttgat
     8101 cagcagctcg acaagagtct gaacatctgt cgatgctgat ggcaacgccg ctgttcgcac
     8161 aggccgctat ccaggagccg gggcgcgttc gcgttctacc atcccaacgc ggacgttctg
     8221 aatggcggcc ggcccacgcc ggcggcctca ggcgccctgg catcggttcc gctcggcgcc
     8281 agcgacgcgt atgccgccat gggcggcaac gcgaacggct cgtcttgcgc tcagcgctat
     8341 cgttcctacg acccggcttc aggtacttgc tcggctatga tggtcaccgg catccgtgcg
     8401 agtgacgcgg ctcgagcgca tgaaagggcg gttgatcgaa ccgcctcttt cgaaatctgc
     8461 catttacaat cggggcctgc cgtattgaac tcatgagatc gcgaaagctt ggagtgtgca
     8521 tctaagtaat tgatatattg agcataaaat cagagctctt gatcgtttgg tcgagctttg
     8581 agcagcttta gcaaccccgt ctccacacgc taaagtggcg gccgtgagag cgggcatcga
     8641 ggctcggtgc gacgtgcttg cgagaaggcc gcgatctgcg atacctcttc attcgcgaaa
     8701 aatcacccac cgccggaccg acaccgcaaa accctccaga cccttgccgg ctagggaatc
     8761 cgcctgtttt ggctttcagc gggaagacgg gtagggtgcc caagtgccct aaaaaggctg
     8821 tgtagatcga aacatgcgca gtttccgggc tgaattccaa atactctcac cgtggcgcga
     8881 atcaacttac aggagacaaa atggcagtac agatgactaa gtcgcagctg atcgagaaaa
     8941 tcgctgttga gaatgaagta ggaaaaaagg acgtaaaggg agtgctggag acgctggcga
     9001 ccatcggcta taaagagctg aagaaaaatg gtgtgttcct ggttcctggc tttgccaagt
     9061 tcgttgtcat caagaaggcc gccaccaagg cgcgcaaggg tgtgaacccc ttcaacggtg
     9121 aacccatgac cttcaaggcg aagcctgctc gaaagatcgt cagagcacgg cccgtcaagg
     9181 ccgcaaaaga cgcggtgtaa atgtcaaaag gccccgacgg ggccttttgc gtttcagcaa
     9241 tggttcgatg cccagggcag cggtgcgaat cgcaagtcca ccgcgccgag cgtgagcgag
     9301 aggccggtcc tggccgtgca agctggtcgc gccaagtctt gaacgtgcca ggtcttgaac
     9361 gtgcaaggtc ttgaacgtgc cacgcaggtg ctggccccgc tcggtctgaa accctcgtca
     9421 tcacgaacgg tcttaccgtg gcaccccgcg tcgaggttcg acgcggcggt cgccgcgcga
     9481 cggcgaattc gatgataaaa aatctggtcc agtcttgctg acgtgatcgc gcggcaatca
     9541 gcagcgcgcg atttaagtcc gcctgctctt catctgtggc cgcgccgcag gcattgcaag
     9601 gcctcttgct gcagcaggat tccctcacta tttgagcccc cgaaggaatc ttgccatgtt
     9661 ggatgttacc cgtgacccat ctggcgatgg gataccgccg caagccgccg agtctgcttc
     9721 cgcgaacgac gcgggtttgc tcgatgctta ttccaatgcc gtcatcgacg ttaccgaacg
     9781 cgtcggcccc gccgtcgtgc gggtcgagac cggatccgga ttgcctggcg cgcgcgagcg
     9841 cggcgggctc ggctccggca tcgtgatctc tcccgacggc ctggtgctga ccaacagcca
     9901 tgtggtcggg tcatcccgac agatccggct cagggacaat gaaggcatcg tcaccgatgc
     9961 gcgcgtgctt ggcgtcgatc ccgataccga tctggcgctg ctgcgcgccg acggcgcgcg
    10021 ggatctgcgc tacgcctcgc tcggcaattc caagaccctg cggcgcgggc aattggtggt
    10081 cgcgatcggt aatcccctgg gcttcgagtc gaccgtgacc gccggcgtgg tctccgcact
    10141 tggccgctcg atccgttcgg tcagcggccg gaccatcgag gacgtgattc agaccgatgc
    10201 cgcgctcaat cccggcaatt ccggcgggcc gctggtgtcg tccgcggccg aggtgattgg
    10261 catcaatacc gccatcatca gcggcgcgca gggcatctgc tttgccgtcg ccagcaatac
    10321 cgcgcagttc gtgctgtcgg aaatcattcg ccacggttat gtgcggcgcg cctatatcgg
    10381 tgtttccggc cagaccgcgc cgattccgcg gaggcatgcg gttgtcgccg gcgtcgacaa
    10441 caagatgggc gcgctgctgg cgcagatcga acctgacggg cctgcggcca gggccgggct
    10501 tttgcccggc gatgtcgtga tccgtctcga tggcgtggag gtcaacggcg tcgacgatct
    10561 gatccgcgcg ctcgatcgcg acaggatcga ccgcacgctc gaaatggatg tgctgcggat
    10621 gggacgtctg cgcaccgtcg agattcatcc ggtcgaacgc aagccggcgg ccgcgcggaa
    10681 atagttgtcg tcgggccgca cgcgatgacg ctcgctaggc cgcgcggtgc agcttatcga
    10741 gcgatgctgc gttagggctt gacgtaaatg ctctcataca ttcctgcgta gtcctccttc
    10801 caacctctct tatccacgag aaaccgcctc aactgttcat gcgccttcac ccaaagcacc
    10861 gtatcgattt ggtatttctc gagcaccgtg tcccaagcgg tctcactaat ctctaattcg
    10921 accagtttga aatagtcacg cagcaaatca tccggataag cggtagctgc ccgaccatct
    10981 acgaacacgg gtacggcacc ttgagtgcgg aaaatcaaaa gt
//
LOCUS       DATNOI010000378         7657 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_537928,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000378 DATNOI010000000
VERSION     DATNOI010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537928"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VN968_15040"
     CDS             <1..670
                     /locus_tag="VN968_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HXL30587.1"
                     /translation="LFAEKLQKKLDYMYFPQATGFVRMTLAAHRCDVIMGFPQGDDLV
                     QGTNPYYRTAYALVAKQGSGLDDVATLEDARLKDKRIGIVAGTPPATNMAVAGLMANA
                     RPYPLMIDTRFDSSSAAMIKDLMSGEIDAGVLWGPMAGYFAKQASPPLHVTPLVKETS
                     GPRLAFRIGMGVRPADQNWKRQLNRLIQENQPAINKILLDFGVPLLDENDKPITAETT
                     TKQP"
     gene            667..1254
                     /locus_tag="VN968_15045"
     CDS             667..1254
                     /locus_tag="VN968_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated CXXCW motif
                     protein"
                     /protein_id="HXL30588.1"
                     /translation="MMLKLARLIAATVAFTVALALPAYAQENAPEPEGYRTDNYRAPV
                     PVTLAGARVLATGEAEAIWRAGAGVFIDVLPHAPKPQNLPAGTIWREKPRLNIPGSIW
                     LPDTGYGTLAAATEDYLRHGLARATGGNSATLLVIYCLTDCWMSWNVAKRALSYGYRN
                     VAWYPEGTDGWQRADLPVAESQPQPRAGEESSSPR"
     gene            complement(1297..1911)
                     /locus_tag="VN968_15050"
     CDS             complement(1297..1911)
                     /locus_tag="VN968_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30589.1"
                     /translation="MNWGIIICTVLAVAGSAERCLAADPRYPDWPCSQAKVPDVSLAA
                     VWAGPPLDDVASKWKDDAKVSALVPRLAARRTPLDEAQKAITEFLEGSAAQKTEMGKL
                     LFAGLFDTLNAQRASVMSGLERVTRKQREAADKIRSDTLALQALQGASPPDQPKLDEL
                     GNQLVWETRIFEDRSRVIKFVCEVPTAIDQRLFALGRVIQQEME"
     gene            complement(1908..2756)
                     /locus_tag="VN968_15055"
     CDS             complement(1908..2756)
                     /locus_tag="VN968_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL30590.1"
                     /translation="MTSAIIKDQPTGFSPAQYLTCLNGIVWREALRFLHQRERFVSAL
                     VRPLVWLFIFAAGFRQVLGISIIPPYETYILYEVYIAPGLMAMIQLFNGMQSSLSMVY
                     DREMGNMRTLLVSPLPRWYLLFCKLLAGTAVSLLQVYAFLLIAWLWDIEPPPIGYLTV
                     LPALVLSGLMLGALGMLISSGIKQLENFAGVMNFVIFPMFFASSALYPLWRVQEGSPM
                     LYYVCLFNPFTHAVELIRFALYGQINWISLAVVGGCTAAFMIGAVFAYDPSRGLSRRG
                     PAGGET"
     gene            complement(2753..3502)
                     /locus_tag="VN968_15060"
     CDS             complement(2753..3502)
                     /locus_tag="VN968_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30591.1"
                     /translation="MPALSIDGVSHSYGARRALIDINFTVAPASFTALLGLNGAGKST
                     LFSLVTRLFGIQAGRIGIFGHDIGREPGEALRLLGVVFQPRTLDLDLSVTQNLLYHAA
                     LHGIDRREARLRAGEVLARIGLADRAGSKVRDLSGGQMRRLEIARALLHRPRLLLLDE
                     ATVGLDVKARADILGHVRRLVAEQGIGVLWATHLFDEIIPSDELVVLHQGQVLAHGPV
                     ARIIADTATQDINTSFKRLTETAAESGSLSP"
     gene            complement(3571..4539)
                     /locus_tag="VN968_15065"
     CDS             complement(3571..4539)
                     /locus_tag="VN968_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YVTN family beta-propeller repeat protein"
                     /protein_id="HXL30592.1"
                     /translation="MRRCLLAGMAAWLAAATPAAAVIAYVSNEKSNTVSVIDTDSWTV
                     TKTIKVGQRPRGIDFTRDGKFVLVAVGDDDTIQVIDAATQQVVDTLPSGPDPELFVQD
                     AAGKVLYVANENDNTVTVIDLEKRVRIGDVQVGVEPEGMAISPDGKILINTSETTNMA
                     HFIDTATRQIVANVLVDARPRFAEFKRDGSELWVSSEIGGTVSIIDPAKREVTGKITF
                     SIPGMRREAIQPVGIGMTKDGKTAFIALGPANRIAIVDGASHQVTKYLLVGQRVWHMA
                     FTPDEKYLLVTNGVSNDVSIIDVVAQKVIKTIQVGELPWGIAIAQP"
     gene            complement(4557..5741)
                     /locus_tag="VN968_15070"
     CDS             complement(4557..5741)
                     /locus_tag="VN968_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30593.1"
                     /translation="MIRLFVGAIGLGVLATSALAADPVEIGIGYLHRAGVKPTLSLVE
                     QPAENDGLAGARLAIEDNNTTGKFLNQHFTLEELRLKDGDDAAKAAAALADRKIGFII
                     ADLPADALLKAADAVRNRGTVLFNAGAIDDRLREQDCRANVIHAAPTRSMLADGLAQY
                     LVWKQWKRWMLAVGSHEEDKLFAEALRRAASRFGAKIVQERVFEDSGGARRTDSGVTL
                     IQRQMPVFTQQAPAYDVLVAADESEVFAPYLPYRTWDPRPVAGSAGLVPKSWDAAQDQ
                     WGAVQMQNRFLKLNSRHMTALDMQAWTAARMIGEAASRKNSGDPKSVLDFLKGPDFSI
                     AAFKGQRLTLRDWNLQLRQPILLADGRMIVSVSPQEGFLHQVSELDTLGLDRPETKCK
                     LH"
     gene            5910..6389
                     /locus_tag="VN968_15075"
     CDS             5910..6389
                     /locus_tag="VN968_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3280 domain-containing protein"
                     /protein_id="HXL30594.1"
                     /translation="MVSAPARADPPKVAVFDFELVDTSLQGEVEGPRADEQGRLMRTG
                     DQLRRALAESGKFVLLDIAPVNAAAHGSHLQACGGCDVQYAQQLGADLAITGMVQKVS
                     NLILNMNVYMRDVHTGHLVTFMSADMRGNTDESWSRAASYLLRNRLLAPDYGAPQPQ"
     gene            complement(6393..7238)
                     /gene="fghA"
                     /locus_tag="VN968_15080"
     CDS             complement(6393..7238)
                     /gene="fghA"
                     /locus_tag="VN968_15080"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HXL30595.1"
                     /translation="MTIETISQNRSHDGVQGVYKHNSRETGTGMTFSVYVPPHHDGAK
                     LPVVWYLSGLTCTHANVTEKGEFRNACAELGLIFVAPDTSPRGEGVPGDPANAYDFGL
                     GAGFYVDATEAPFARHYRMWSYVTKELPELIAEHFPVDPKRQSILGHSMGGHGALTIA
                     LSHPDRYRAASAFSPIVAPSEVPWGIKALSGYLGADRKAWREHDTVALIAAGARFSDL
                     LVDYGDADPFLTEQLRPELLERACAQAEIPLTLRCQPGYDHSYYFISTFMNDHLRWHA
                     ARLRA"
     gene            complement(7345..>7657)
                     /locus_tag="VN968_15085"
     CDS             complement(7345..>7657)
                     /locus_tag="VN968_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045861.1"
                     /note="catalyzes the reaction of formaldehyde with
                     glutathione to form S-hydroxymethylglutathione; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde-activating
                     protein"
                     /protein_id="HXL30596.1"
                     /translation="DAAAAIQRHACKVCGTHMYGRIENKAHPFYGLDFIHPELFQEQG
                     SAAPGFAAFVSSVIESGVKPDEMPGIRSRLKELGLEPYDCLSPALMDAIATHVAKSKA
                     A"
BASE COUNT         1445 a         2351 c         2453 g         1408 t
ORIGIN      
        1 actgttcgcc gaaaagcttc aaaagaagct ggattatatg tactttccgc aagcgaccgg
       61 tttcgtccgg atgacgcttg ccgcccatcg ttgcgacgtc atcatgggat tcccgcaagg
      121 cgacgatctc gttcagggca ccaatcccta ttaccgcacg gcctacgcgc ttgtcgccaa
      181 gcagggcagc ggtctcgatg atgttgcgac gcttgaagac gcgcgtctca aggacaaacg
      241 cattggcatc gttgccggca cgccgcctgc caccaacatg gcggtcgccg gcctgatggc
      301 gaatgccagg ccttacccgc tgatgatcga tacccgcttc gattcttcgt cagcggccat
      361 gatcaaggat ctgatgtctg gggagatcga cgccggcgtt ctgtgggggc cgatggcggg
      421 ctatttcgcc aagcaggcca gcccgccgct ccacgtcacc ccactggtca aggaaacctc
      481 gggaccgcgc cttgcttttc ggattggcat gggtgtccgg ccggccgatc agaactggaa
      541 acggcagctc aaccgcctga tccaggagaa ccaaccggcc atcaacaaga tactgctcga
      601 cttcggcgta ccgctgctcg acgaaaacga taagccgatc accgcggaga cgacgacgaa
      661 gcagccatga tgttgaagct tgcgcgcctg atcgccgcca cggttgcctt tacggttgcc
      721 ttggcgctgc cggcgtacgc ccaggaaaac gctcccgagc ccgagggata ccgcaccgat
      781 aattatcgcg cacctgttcc ggtaaccctc gccggcgcgc gcgttctggc gaccggagag
      841 gccgaagcca tctggcgcgc gggggcgggt gtattcatcg atgtgctgcc gcatgcgccg
      901 aagccgcaaa accttcccgc aggcacgatc tggcgcgaga agcctcggct caatatccct
      961 ggcagcatct ggctgccgga caccggttac ggaaccctcg ccgcggcgac cgaagattat
     1021 ctgcggcacg gacttgcccg tgctaccggc ggcaacagcg cgacgctgct cgtgatctac
     1081 tgcctgaccg attgctggat gtcgtggaac gtggccaaac gggcgttgtc ctacgggtat
     1141 cgcaacgtgg cctggtatcc cgaagggact gacggttggc agcgcgccga tttgccggtg
     1201 gccgaatcgc agccgcagcc acgggcaggt gaggagagct catcgccgcg ttaaccggcg
     1261 ggcagcaccg gcaagcggcc ggcggcacgc ggcgcgctat tccatctcct gctggatcac
     1321 acggccgagc gcgaacagcc tttggtcgat cgccgtcggt acctcgcata cgaatttgat
     1381 cacccggctg cgatcctcga agatacgtgt ttcccagacc agttgattgc cgagttcgtc
     1441 gagcttgggc tgatcgggcg gggacgcacc ctgcagggcc tgcagcgcaa gcgtgtcgct
     1501 ccgaatcttg tctgctgcct cgcgctgttt gcgggtcacc cgttcaagcc cgctcatgac
     1561 agacgcgcgc tgcgcattga gcgtgtcgaa caggccggcg aacaacagct tgcccatctc
     1621 ggtcttttgc gccgccgagc cctcaagaaa ttccgtgatc gccttctggg cttcatcgag
     1681 cggcgtgcgc cttgcggcca gccgcggcac cagtgcgctc accttggcgt cgtccttcca
     1741 tttactcgcg acgtcgtcga gcggcggacc tgcccacacc gcggcgagcg agacatccgg
     1801 caccttggcc tgggagcacg gccagtctgg atagcgcgga tccgcggcga ggcagcgttc
     1861 ggccgatccg gcgaccgcga gcaccgtgca gatgatgatg ccccaattca tgtctcgcct
     1921 ccggcgggtc cgcggcggct gaggccgcgc gacggatcat aggcgaacac tgccccgatc
     1981 atgaaggccg ccgtgcagcc gccaaccacc gccagcgaga tccagttgat ttgtccgtag
     2041 agcgcgaagc ggatcaactc gaccgcatgg gtgaatggat tgaacaggca aacgtaatag
     2101 agcatcgggc ttccctcctg tacccgccat agcggataaa gcgcggacga agcgaaaaac
     2161 atcgggaaaa taacgaagtt catcacgccg gcgaaattct cgagttgctt gatgcctgaa
     2221 gaaatcagca tgccgagcgc cccgagcatc aggcccgaca ggaccagcgc cggcaggacc
     2281 gtgagatagc cgatcggcgg cggctcgata tcccagagcc atgcaatcag caggaaggca
     2341 tagacctgga gcagcgagac cgcggttccc gccagaagct tgcagaacag cagataccag
     2401 cgcggcagcg gactcaccag cagggtgcgc atattgccca tctcgcggtc gtagaccatc
     2461 gagagcgagg actgcatgcc gttgaacagc tggatcatcg ccatcagtcc aggcgcgata
     2521 tagacctcgt aaaggatgta ggtttcgtag ggcgggatga tggagatgcc gagcacctgg
     2581 cggaagccgg ccgcgaagat gaacagccac accagcggcc gaaccagcgc cgagacgaag
     2641 cgctcacgct gatgcaggaa gcgcagggcc tcgcgccaca cgatgccatt caggcaggtg
     2701 aggtactgcg cgggcgaaaa cccggtcggt tgatctttga tgatggcgga ggtcatggcg
     2761 aaaggctccc ggactctgcc gcggtttcgg tgaggcgctt gaatgaggtg ttgatgtcct
     2821 gtgttgcggt gtcggcgatg atgcgtgcga ccggaccatg cgcgagaacc tggccctggt
     2881 gcagtaccac gagttcatcg ctggggatga tctcgtcgaa caaatgggtg gcccacagca
     2941 cgccgatgcc ttgttcggcg accagccggc ggacatggcc gaggatatcg gcgcgcgcct
     3001 tgacgtcgag gccgaccgtc gcctcgtcaa gcagcagcag gcgaggacgg tgcagcaagg
     3061 cgcgcgcgat ttccagccgc cgcatctggc cgcccgagag gtcgcgcacc ttgctgccgg
     3121 cgcggtcggc aaggccgatg cgcgccagca cttcaccggc gcgcaagcgc gcctcgcgcc
     3181 gatcgatgcc gtgcagggca gcgtgataca gcagattttg cgtgaccgag agatcgaggt
     3241 cgagcgtgcg cggctggaac accacgccca gcagccgcag cgcttcgccg ggctcacggc
     3301 cgatgtcgtg gccgaagata ccgatgcgtc cggcctggat cccgaacagc cgcgtgacca
     3361 gcgagaacaa cgtgctcttg ccggcgccgt tgaggccaag cagcgccgtg aagcttgccg
     3421 gcgcgacggt gaagttgatg tcgatgaggg cgcgtcgggc gccataggaa tggctgacgc
     3481 cgtcgattga cagcgcgggc atttcggccg gactgctgcg gcggtgactg tccggcggag
     3541 tctgtccggc gaggggcgcg tcggttgcgg tcatggctgc gcaatcgcga tgccccaggg
     3601 cagctcgccg acctggattg tcttgatcac cttctgcgca acgacatcga tgatcgaaac
     3661 gtcgttggat acgccgttgg tcaccagcag atatttttca tccggggtaa atgccatgtg
     3721 ccagacccgc tgtccgacca gaaggtattt ggtgacctga tggctggcgc catcgacgat
     3781 cgcgatccgg ttggcagggc caagcgcgat gaaagcggtc ttgccatctt tggtcatgcc
     3841 gatgccaacc ggctggatgg cctcgcgccg catgcccgga atgctgaacg tgatcttgcc
     3901 ggtaacctcg cgttttgccg ggtcgatgat ggacaccgtg cccccgatct ccgaggacac
     3961 ccacagctcg gagccgtcac gcttgaactc ggcgaagcgg ggccgcgcat cgaccagcac
     4021 gttggcgacg atctggcgcg tcgcggtgtc gatgaagtgc gccatgttgg tggtctctga
     4081 tgtgttgatc agaatcttgc cgtccgggct gatggccatg ccctcgggct cgacgccgac
     4141 ctggacgtcg ccgatgcgca cgcgcttttc gagatcgatg acggtgaccg tgttgtcgtt
     4201 ctcgttggcg acatacagca ctttgccggc ggcatcctgc acgaacaact cgggatcggg
     4261 accggagggc agggtgtcca ccacctgttg ggtcgcggcg tcgatcactt gaatggtgtc
     4321 gtcatcgccg accgcgacca gcacgaactt gccgtctctg gtaaagtcga tgccgcgcgg
     4381 gcgctggccg accttgatgg ttttggtgac ggtccagctg tcggtatcga tcaccgatac
     4441 cgtgttgctc ttttcattcg acacataggc gatgacggcc gccgcgggcg tggcggccgc
     4501 aagccaggca gccattccgg caagcagaca acgacgcatg ggcgatctct ccatcttcaa
     4561 tgcagcttgc atttggtttc aggccggtcg aggccgagcg tatcgagttc cgagacttga
     4621 tgcaggaagc cttcctgcgg cgagaccgat acgatcatgc ggccgtccgc cagcagaatt
     4681 ggctggcgaa gctgcaggtt ccaatcccgc agcgtcagtc gctgaccctt gaaggcggcg
     4741 atggaaaaat ccggtccctt gagaaagtcg agcaccgatt tcggatctcc ggaatttttg
     4801 cgcgacgcag cctcgccaat catgcgcgct gcggtccatg cctgcatgtc cagcgccgtc
     4861 atatgccgcg aattcaactt cagaaagcgg ttttgcatct gtaccgcgcc ccactggtcc
     4921 tgagcggcgt cccagctttt cggaacaagg ccggcggatc ccgcgaccgg cctcgggtcc
     4981 caggttcggt agggcaggta cggcgcgaat acctcgctct cgtctgcggc gacgagaacg
     5041 tcataggcgg gagcttgctg ggtgaacacg ggcatttgac gctggatcaa ggtcacaccg
     5101 ctatcggtgc gacgtgcgcc gccgctatcc tcgaatacgc gctcctggac gatcttggcg
     5161 ccaaagcgcg aggcggcgcg tcgcagcgct tcggcaaaga gcttgtcttc ctcgtgcgat
     5221 cccaccgcca gcatccagcg cttccattgt ttccatacaa gatattgggc gagaccgtcc
     5281 gcgagcatcg aacgcgtggg cgcagcatgg atcacgttgg ctcggcaatc ctgctcgcgc
     5341 aagcgatcgt cgatcgcgcc ggcgttgaac aacacggttc cgcgattacg gactgcgtcg
     5401 gcggccttca gcagcgcgtc ggcggggaga tcggcaatga tgaagccgat cttgcgatcg
     5461 gcgagcgcgg ccgccgcctt ggctgcgtcg tcgccgtcct tcagccgaag ctcttccagg
     5521 gtgaagtgct gattgaggaa cttgccggtg gtgttgttgt cctcgatcgc gagacgggcg
     5581 cccgccagac cgtcgttctc ggccggctgc tcgaccagcg atagtgtcgg cttgacaccg
     5641 gcccgatgaa ggtagccgat tccgatctcg acgggatcgg ccgcgagggc gctggtagcc
     5701 agcacaccca agccgatcgc gccgacgaac aaccggatca tgttttctcc ctgacattcc
     5761 tcttcgactg taccgacgat agagggactt ccagcttgaa acatgaccga attgtcctac
     5821 cttgcaaccg gcattttgcc actgtgctgc cgtggatttc gcgggtcccg atcatgaaac
     5881 ctccgttcgt cctcatggcg ttgacgctga tggtctcagc gccggcgcgt gccgacccgc
     5941 ctaaagttgc ggtgtttgat ttcgaactgg tggacaccag ccttcagggc gaagtagagg
     6001 ggccgcgggc ggacgagcag ggacggctga tgcgcacagg cgaccaactg cgcagggcgt
     6061 tggcggaatc cggcaagttc gtcctcctcg acattgcgcc ggtcaacgct gcggcgcatg
     6121 gcagccattt gcaggcctgc ggcggctgcg acgtgcaata cgcacagcag ctcggcgctg
     6181 atcttgccat caccggcatg gttcaaaagg tctcgaacct tattctcaat atgaatgttt
     6241 acatgcggga cgtccatacc ggacatctcg ttaccttcat gagcgcggat atgcgcggca
     6301 acaccgacga atcctggtcg cgtgcggcga gctatctctt gcgcaaccgg ctgctcgcgc
     6361 ccgattacgg cgcgccgcaa ccgcaataag cgctaagctc tcaaacgcgc ggcgtgccag
     6421 cgcagatggt cgttcatgaa ggtggagatg aagtagtagc tgtggtcgta gcccggctga
     6481 caacgcaagg tgagcgggat ttcggcctgc gcgcaagcac gctccagcag ttccgggcgc
     6541 agctgttcgg tgaggaatgg gtcggcatca ccgtaatcta ccagaagatc ggaaaaccgg
     6601 gcgccggccg cgatcagcgc gacggtgtca tgctcgcgcc atgccttccg gtcggcgccg
     6661 agatagccgc tcagggcttt gatgccccac ggcacctcgg agggggcgac gatgggcgag
     6721 aaggcactgg cggcgcggta gcgatcggga tgggagagtg caatcgtcag cgcgccatgg
     6781 ccgcccatcg aatgaccgag gatcgattgc cgtttgggat ccacgggaaa atgctcggca
     6841 atcaattcgg gcagttcttt cgtcacatag ctccacatcc ggtaatggcg cgcgaatggc
     6901 gcttccgtgg catcgacata aaagccggca ccgagtccga aatcataggc attggcggga
     6961 tcgcccggca caccttcgcc gcgcgggctg gtatccggtg cgacaaagat caggcccaac
     7021 tcggcgcatg cgtttcgaaa ttcgcccttt tcggtgacgt tggcatgggt gcaggtcaga
     7081 ccggacagat accagaccac cggtagcttc gcgccgtcat gatgcggagg aacgtagacc
     7141 gaaaaagtca tgccggtccc ggtctcacgg ctattgtgct tgtaaacacc ctgcacgccg
     7201 tcgtgcgatc gattttggga aatggtttcg atcgtcatga tgtcaagact ccggcgtggc
     7261 aacgacgcaa gggttgccaa gggtcaaacg tgatagcggc tggcagagcg cgccgccagc
     7321 cgcaatctac acgaggttgc cggatcaggc agctttggat ttcgccacat gggtggcgat
     7381 cgcatccatc agggctggcg acaggcaatc atagggctcg agtccgagtt ccttgagacg
     7441 cgaacggatt ccgggcatct cgtccggttt gacgccggac tcgatcacgg acgatacgaa
     7501 cgccgcaaat cccggcgcgg ccgagccttg ctcctggaac agttcgggat ggatgaagtc
     7561 gaggccgtag aacggatgcg ccttgttctc gatgcgcccg tacatatggg tgccgcagac
     7621 cttgcaggcg tgccgctgga tcgccgccgc ggcgtcg
//
LOCUS       DATNOI010000379         5435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1466840,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000379 DATNOI010000000
VERSION     DATNOI010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5435
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466840"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VN968_15090"
     CDS             complement(<1..219)
                     /locus_tag="VN968_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HXL30597.1"
                     /translation="MFDNLSEKLGGILDRLTRRGSLTEADVDAAMREVRRALLEADVS
                     LDVVRSFTEKVREQAIGATVVKSVTPGQM"
     gene            678..1742
                     /locus_tag="VN968_15095"
     CDS             678..1742
                     /locus_tag="VN968_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL30598.1"
                     /translation="MKMTRRRVLGLLAGAAAVVAVPSVWITRMKTYDGPVSDHFDGTH
                     FFDPDGSPPKSLGEVLRWQFGPGRERQAWPEWAPSPHADTPPPRVTGEKVRLSFVGHA
                     SWLIQTSGLNILIDPVWSTRASPLSFAGPKRHNDPGIAFDALPPIDVVLVSHGHYDHL
                     DLVTLSKLAASFSPRVITPLGNDVTMRGADSAIKAEAFDWHDRVELGSGIAVTLVPTR
                     HWTARGLFDRNKALWASFVLQTPAGKVYVVCDSGYGEGKHFRRVAEAHGPLRLAILPI
                     GAYEPRWFMRDQHMNPSDAVKALADCGAAQALAHHHGTFQLTDEAIDAPVIALQAALE
                     EARIPRERFAVLKPGQVVEI"
     gene            complement(1868..2569)
                     /locus_tag="VN968_15100"
     CDS             complement(1868..2569)
                     /locus_tag="VN968_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023489.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HXL30599.1"
                     /translation="MKHRTVLAVALAAGTLAAAPALAQGIPPAAISVTGEATVSVPPD
                     LAEVDGGVASEAKTAREASEANSAAMAKVLAALKGAGIDAKDIQTSRLSLQPQSAPNR
                     TGPSAIVGYRASNRVTIRVRDVTKVANVIDTLVGAGANEIGGINFMVSQASKLLDEAR
                     EQAVADARRKAEIYAKAAGVTLGAPLGISEEGSPGPVPFRKMAAGMAVSAPVAQGEET
                     LQVTVSVSWAIKQAQ"
     gene            complement(2575..3273)
                     /locus_tag="VN968_15105"
     CDS             complement(2575..3273)
                     /locus_tag="VN968_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HXL30600.1"
                     /translation="MNRPRLVRLAMVALIPAAISLAMGNVQAQSPPAATPPPAAAPAP
                     APTATPAPAASPSAAPPAAAPPAAAETPAAAPVQTADPFGEEITLTQKPVVIIKGNAN
                     WDSAFDTLMESFKSLSALLDKQGIKPTGNSMIVYTSTDDTGFTYLAEMPVDQDPKNLT
                     KDMSVGKSPEGKALKFVHRGSYDNMDNTYEAITNHLDDKKLEAKDTFIEEYITDPLKT
                     AEDKLVINVYVPLK"
     gene            3493..4365
                     /gene="dapF"
                     /locus_tag="VN968_15110"
     CDS             3493..4365
                     /gene="dapF"
                     /locus_tag="VN968_15110"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXL30601.1"
                     /translation="MSALANHAFAKMNGIGNEIVVVDLRDRPATVTAEEARAVASPAG
                     VHYDQLMVLQPPRLPGTEAFIRIYNNDGSEAAACGNGMRCVARRLFEHSGQTAATFET
                     QAGLLNCWQGPSGDLYTVDMGPPKFGWKDIPLAEEFRDTRYIELQVGPIDAPVLHSPS
                     VVSMGNPHAIFWVDDVNAYDLARFGPLLESHPIFPERANITLAHIVDRDHIAIRTFER
                     GAGLTKACGSAACATAVAAARLKRANRIVGISLPGGDLSIEWREGDDHVLMTGTATFE
                     YEGRFDPALFVGVA"
     gene            4367..>5435
                     /gene="mtaB"
                     /locus_tag="VN968_15115"
     CDS             4367..>5435
                     /gene="mtaB"
                     /locus_tag="VN968_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HXL30602.1"
                     /translation="MSVDVVTFGCRLNAFESEVIAREAERAGLTDTVVINSCAVTNEA
                     VAQARQSIRRLKRERPEARIVVTGCAAQTQALMFAEMTEVDRVVGNDDKMRGEAWRHT
                     RAAFDAAPAFGIASEEKIAVADIMAVKEMAPHLLEGFQKGLPRVFVQVQNGCDHRCTF
                     CIIPYGRGNSRSVPMGAVISQVRALVERGHAEIVLTGVDLTSYGADLPGTPKLGQLTR
                     QILRHVPELKRLRISSIDHVEVDRDLLDAIADDERLMPHLHLSLQSGDDLILKRMKRR
                     HSRKEAVEFCAQVRRLRPDIALGADIIAGFPTETEEMFERSRTLVDECDLTFLHVFPY
                     SPRPGTPAARMPQVAGPAIKDR"
BASE COUNT          910 a         1744 c         1766 g         1015 t
ORIGIN      
        1 catctggccg ggggtcaccg acttgaccac ggtggcgccg atcgcctgct cgcggacttt
       61 ttcggtaaaa ctccggacta cgtcgagcga gacgtcggct tccagcaatg cgcggcgcac
      121 ctcgcgcatc gcggcatcga cgtcggcttc cgtcagcgac ccgcgccggg tcagccgatc
      181 gagaatccca ccaagctttt ccgacagatt gtcgaacact gctgcttttc cttcgtcatg
      241 cccagatggg caccgcatcg cctatttcac cgccgtcata ccccgccccc gggtcggcgc
      301 taagcgccgc ccgatgacag gctccatcgg ggtatccagt attcggaggc ttcgctttaa
      361 aacgaacgcc cttgggatac tggatcaccc gctttcgcgg gtgatgacac cacgtcttgt
      421 ctcgctcgcg cctcagggac gagagaatga tattccaaac acctttgcgc ccgagggcgc
      481 atcgcgctgt cgggcgttga cctccggtct caggggccgg tcggtgggtc gaaaagaacg
      541 tcttttcgag gattggcgcg gttaaacccc cgcagaccat gaaagtcaag aaaagccgcg
      601 gcaaaatggc gttttcgtga acccggccgg tcgcccaccc ccttgccgat gcgcgccttt
      661 ttggggtcaa tcggaccatg aaaatgaccc gccgtcgcgt gcttgggctg ctggccggcg
      721 ctgccgccgt cgtcgccgtg ccctcagtct ggattacccg catgaaaacc tacgacggcc
      781 ctgtctccga tcatttcgac ggcacgcatt ttttcgatcc cgatggctcg ccgccgaaat
      841 cgctcggcga ggtcttgcgc tggcagttcg gtcccggcag ggagcggcag gcctggccgg
      901 aatgggcgcc gagcccccat gccgacacgc cgccgccgcg cgtgaccggc gaaaaggtga
      961 gattatcatt tgtcggtcac gccagctggc tgatccagac ttccgggctg aacatcctga
     1021 tcgatccggt gtggtcgacg cgcgcctcgc cgctgagctt tgccgggccg aagcgtcaca
     1081 acgatcccgg cattgcgttc gatgcgctgc ctccgatcga cgtcgtgctg gtgtcgcatg
     1141 gacattacga tcatctcgac ctcgtgacgc tgtcgaagct cgcggcaagc ttttcgcctc
     1201 gtgtgatcac gccgctcggc aacgatgtca ctatgcgcgg cgccgacagt gcgatcaagg
     1261 ccgaggcgtt cgactggcat gaccgggttg aacttggaag cggcatcgcg gtgacgctgg
     1321 tgccgacccg gcactggacg gcgcgcggat tgttcgaccg caacaaggcg ctgtgggcga
     1381 gcttcgtgct gcagacgccg gcgggcaagg tctacgtcgt ctgcgattcc ggctatggcg
     1441 agggcaaaca ctttcgccgc gtcgccgagg cgcacggccc gttgcggctg gcgatcctcc
     1501 cgatcggcgc ctatgagccg cgctggttca tgcgcgacca gcacatgaac ccgtccgacg
     1561 ccgtaaaggc gctggccgat tgcggtgcgg cgcaggcgct ggcccatcac cacggcacct
     1621 ttcaactgac cgatgaggcg atcgatgcgc cggtcatcgc attgcaggcg gcgctcgagg
     1681 aagccaggat tccgcgcgag cggtttgcgg tgctcaagcc ggggcaggtg gtggagattt
     1741 agtcgtcgtc ccggcgaagg ccgggaccca taaccaccga tctcgcttgt ttgcagaagg
     1801 cggttgctcc attgcccaaa acgaaacggc acggcgtctg ggtcccggcg tccgccggga
     1861 cgacgtctta ttgtgcctgc ttgatcgccc acgacacgct caccgtcact tgaagcgtct
     1921 cttcgccctg cgccaccggt gctgaaaccg ccatgccggc cgccatcttg cgaaatggca
     1981 ccgggccggg cgaaccttct tccgagatgc ccagcggcgc gccgagcgtg acgccggcgg
     2041 ccttggcata aatctcggcc ttgcggcggg catcggccac cgcctgttcg cgggcctcgt
     2101 cgagcagctt tgaagcctgc gacaccatga aattgatgcc gccgatttcg ttggcgcccg
     2161 cgccgaccag cgtgtcgatt acgttcgcga ccttggtgac gtcgcgcacg cggattgtca
     2221 cgcgattgct ggcgcggtag ccgacaatgg ccgacggacc ggtgcggttc ggggcgcttt
     2281 gcggctgcag cgacagccgc gaggtctgga tatcctttgc gtcgatgccg gcgcccttca
     2341 gcgcggccag caccttggcc atggccgcgc tgttggcctc cgacgcctcg cgcgcggtct
     2401 tggcctccga agcgacgccg ccatcgacct ctgccagatc cggcggcacc gataccgtgg
     2461 cttcgccggt gaccgaaatc gctgccggcg gaatgccctg cgccagtgcg ggcgctgcgg
     2521 ccagcgtgcc cgcggcgagg gcaaccgcga gaaccgtgcg atgcttcatg ggccttactt
     2581 cagcggcaca tagacgttga tcaccagttt gtcctccgcg gttttcaagg ggtcggtgat
     2641 gtattcctcg atgaaggtat ccttggcttc gagcttcttg tcgtcgaggt gattggtgat
     2701 ggcctcatag gtattgtcca tattgtcgta ggaaccgcga tggacgaact tcagcgcctt
     2761 gccctcgggc gatttgccca cgctcatgtc cttggtcaga ttcttcggat cctgatcgac
     2821 cggcatttcc gcgaggtagg tgaagccggt atcgtcggtg gaggtataga cgatcatcga
     2881 attgcctgtc ggcttgatgc cctgcttgtc gagcaaggct gacagcgact tgaacgattc
     2941 catcaaggtg tcgaacgccg aatcccagtt ggcgttgccc ttgatgatga cgaccggctt
     3001 ctgcgtcagg gtgatttcct cgccgaacgg atccgcggtc tgaaccggcg cggcagccgg
     3061 ggtttctgcg gctgcgggcg gcgcggctgc aggcggagcc gctgatgggc tggctgccgg
     3121 agccggggtt gcggtcggcg ccggggcggg cgccgcggca ggtggtggcg tcgccgcagg
     3181 cggcgattgc gcctgcacgt tgcccatagc cagcgaaatc gcggccggga tcagcgcgac
     3241 catggcaagg cggacgaggc gggggcgatt cattctttat tctccatggc cgtatcctgc
     3301 ggtcgcgtcc tggcttgcgc gtgtcccggc tgacgccgaa gacatcgcca ttctaacacg
     3361 cgaggcgtgc tttagtccca tgacagaaaa ttcatgggcc tccgctgcca gcagggagcc
     3421 gacacagatt tgccgcactg gccaagccgc cctcgttcgc catataagac aggcaccagt
     3481 cgggaaaatt ccatgagcgc gctcgccaat catgcgtttg ccaagatgaa cggcatcggc
     3541 aacgagattg tcgtggtcga tctgcgcgac aggcccgcca ccgtcacggc cgaggaggcg
     3601 cgcgcggtag cctcgcccgc gggcgtgcat tacgaccagc tgatggtgct gcagccgccg
     3661 cgcctgcccg gcaccgaggc cttcatccgc atctataaca atgacggttc ggaagcggcg
     3721 gcctgcggca acgggatgcg ctgcgtggcg cgccggctgt tcgagcacag cggccagacc
     3781 gccgccactt tcgagaccca agccgggctg ctgaattgct ggcagggtcc gtccggcgat
     3841 ctctacaccg tcgacatggg cccgccgaaa ttcggctgga aggatattcc gctcgcggaa
     3901 gagtttcgcg atacccgcta tatcgaatta caggtcgggc cgattgacgc gccggtgctg
     3961 cattcgccct ccgtggtcag catgggcaat ccgcatgcga tcttctgggt cgacgacgtc
     4021 aacgcctacg atctcgcccg cttcgggccg ctgttggaaa gccatccgat ctttccggag
     4081 cgcgccaata tcacgctggc gcatatcgtc gatcgcgacc atatcgcgat ccgcaccttt
     4141 gagcgcggcg cggggctcac caaagcctgc ggctcggcgg cctgcgcgac cgccgtcgcg
     4201 gcggcgcggc tgaagcgcgc caatcgcatc gtcggaatat cgttgcccgg cggtgacctt
     4261 tccatcgaat ggcgcgaggg tgacgaccac gtcctgatga cgggcacggc gacgttcgag
     4321 tatgaggggc gattcgatcc ggcactattt gtcggcgtcg cctgagatga gcgttgacgt
     4381 cgtcaccttc ggctgccgcc tcaacgcgtt cgaatccgag gtgattgcgc gggaggctga
     4441 gcgcgccggg ctcaccgata ccgtcgtcat caacagttgc gcggtgacca acgaagccgt
     4501 cgcgcaggcg cggcaatcga tccggcggct gaagcgcgag cggccggaag ctcggatcgt
     4561 ggtgacaggc tgcgcggcgc agacgcaagc cctgatgttt gcggagatga cagaggtcga
     4621 ccgcgtcgtc ggcaatgacg acaagatgcg cggcgaggcg tggcgccata cgcgcgccgc
     4681 gttcgatgct gcgccggcat tcggcattgc cagcgaggaa aagatcgcgg tcgccgacat
     4741 catggcggtc aaggagatgg cgccgcatct cctggaggga tttcagaaag gcctgccgcg
     4801 ggtattcgtg caggtgcaga acggctgcga tcaccgctgc acgttctgca tcatccccta
     4861 tggccgcggt aattcgcggt cggtgccgat gggcgcggtg atcagccagg ttcgcgcgct
     4921 ggtcgagcgt ggccacgccg aaatcgtgct gaccggggtc gatctgacga gctatggcgc
     4981 cgacctgccg ggcacgccga aactcggcca gctgacaaga caaatcctgc ggcacgtgcc
     5041 cgaattgaaa cggctgcgca tctcatcgat cgatcacgtc gaggtcgatc gcgatctgct
     5101 cgacgcgatc gcggatgatg aacgcctgat gccgcacctg catctgtcgc tgcaatccgg
     5161 cgacgacctg atcctgaagc ggatgaagcg ccgccattcc cgaaaggagg cggtggaatt
     5221 ctgcgcgcag gtgcgccgat tgcggccgga catcgcactc ggcgccgata tcatcgccgg
     5281 atttccgacc gagacggaag agatgttcga acgatcgcgg accctcgtgg acgaatgcga
     5341 cctcactttc ctgcacgtgt ttccctattc accccggccc ggcacgccgg ccgcccgcat
     5401 gccgcaggta gcggggcctg caatcaagga ccgcg
//
LOCUS       DATNOI010000380         4805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_858, whole
            genome shotgun sequence.
ACCESSION   DATNOI010000380 DATNOI010000000
VERSION     DATNOI010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..564
                     /locus_tag="VN968_15120"
                     /pseudo
     CDS             46..564
                     /locus_tag="VN968_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
     gene            669..1169
                     /locus_tag="VN968_15125"
     CDS             669..1169
                     /locus_tag="VN968_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXL30603.1"
                     /translation="MSDTPSHGPVKSIMPKWVRGPQDFVGGIALIAIAVFALWASSDL
                     QGMHGFSFGAGTAPRMFAVLLLGLGFAVMVVGLVAEGPHLATYAWRGPLFVSLSILSF
                     AVTIRPLGLVLSAFASFMISALGTPETRWKETIIVGICLTIGCSLLFPYALGLPMQLF
                     PRFLVQ"
     gene            1177..2694
                     /locus_tag="VN968_15130"
     CDS             1177..2694
                     /locus_tag="VN968_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXL30604.1"
                     /translation="MVELFHNLALGFGVAFSPINLLLCLIGALVGTLVGVLPGIGTIA
                     TVAMLLPITFGLPPVGALIMLAGIYYGAQYGGSTTSILVNIPGEATSVVTTLDGFQMA
                     KQGRAGPALAIAAIGSFFAGCVATVLIAVLGTPLTKLALAFGPAEYFSLMVLGLIFAT
                     VLAKGSVLKAIAMIVFGLLLSMVGSDIETGASRMAFNVPELADGLGFATVAMGVFGFA
                     EIIRNLDAGAEMDRHLVQQKITGLMPTKKDLIASAPAIVRGTILGSILGILPGGGAVI
                     ASFAAYTFEKKIAKDPSRFGRGAIEGVAAPESANNAAAQTSFIPLLTLGIPPNAVMAL
                     MVGAMTIHGIVPGPQVMQKQPELVWGMIASMWIGNLMLIIINLPLVGIWVRLLRVPYR
                     LMFPSIVIFCAIGIYSINNAPVDVILAGAFGLLGYWLIKHDFEPAPLLLGMVLGPLME
                     ENLRRALLISRGDATVFFTRPLSAALMAMAAFLLVLALLPSLRKKRDEVFVESET"
     gene            2880..3674
                     /locus_tag="VN968_15135"
     CDS             2880..3674
                     /locus_tag="VN968_15135"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HXL30605.1"
                     /translation="MNQYHDLLERILSDGAEKHDRTGTGTLSIFGHQMRFNLAAGFPM
                     LTTKKLPLKSIVHELLWFLKGDTNIKYLRDHGVSIWDEWADANGDLGPVYGSQWRSWP
                     APDGRSIDQISNVIAMIRRNPDSRRLIVSAWNPADVDKMALPPCHCLFQFYVANGKLS
                     CQLYQRSADVFLGVPFNIASYALLTLMVAQVTDLKPGDFVHSLGDAHLYSNHLEQARL
                     QLTRPTRPLPTMKINPEVKDIFAFRYEDFVLEGYDPHPHIKAEVAV"
     gene            3804..4325
                     /locus_tag="VN968_15140"
     CDS             3804..4325
                     /locus_tag="VN968_15140"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HXL30606.1"
                     /translation="MSFPEIVLVVAVADNGVIGSRGALPWRLKSDIQRFKAITTGKPV
                     VMGRKTFASIGRPLPGRTNIVVTHDANFRARGAVVVTSFAGALAVALGDALRRWATDI
                     AVIGGAEIYEQWLDIADRLEVTEVHARPEGDTHFAAIDAANWEEVARVRNPAGPDDSA
                     DFSYVTYRRRRSH"
     gene            4486..>4805
                     /locus_tag="VN968_15145"
     CDS             4486..>4805
                     /locus_tag="VN968_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK N-terminal
                     domain-containing protein"
                     /protein_id="HXL30607.1"
                     /translation="MPWKNQGGGPWGSGPKGPWGSGPQPVGPRPPDLEDLLRRAQDRL
                     QQLLPGGYFSGLGIALLLIGALAIWGLSGFFRVQSEELGVVLRFGKHVRTVQPGLNYH
                     LPYP"
BASE COUNT          795 a         1428 c         1540 g         1042 t
ORIGIN      
        1 gctcagcatt gcgctcagct ttgcgatgcg ccggcgagtt ccggcatgaa ttccgactcc
       61 aacccggttt tgacgcttgc ctggacattc ggcctgatgt cgctgtttgc ggtgggaggc
      121 gcgaatgcgg ccatcccgga aatgcaccgg attgcagtcg atgtgcagca ctggatgacc
      181 gacaagcaat ttgccgatgc cttcgcaatc tcgcagatgt cgccgggccc gaacgtgctg
      241 atcgtcaccc tgattggcta taccgtcgcc ggtgttgcag gcgcgctggt cgcaacgctg
      301 gcgatgtgcg ttccgacggc ggtgcttgcc tattgtgtca gccgcctgct cacgcgatcg
      361 agccactcgc gttggcccgc catcattcag gcggcgctgg ttccactctc gataggattg
      421 atgggcgcca gtggcctgat cctggcgctg acctccgatc ggacctggac cggtgccctg
      481 gttaccgccg tatcggcggt gctggctttt gcgacgcggc tcaatccgct ctggctgttg
      541 ctggccgggg gggtgtttgg gttttgctgg cgttatctga cgaaaatcgc taggcaaggc
      601 ttgggcgggc agcatcaacg ctatccaaat agaatgggcg agaagcccag tgccagggag
      661 agagatcgat gagcgatacg ccgagccatg ggccggtcaa atccatcatg ccgaaatggg
      721 ttcgtggtcc gcaggatttc gtcggcggga tagcgctgat tgcgatcgcg gtattcgcct
      781 tgtgggcatc gagcgatctg cagggcatgc atgggttttc gttcggggcc ggaaccgcgc
      841 cccgcatgtt tgccgttctc ttgttagggc taggcttcgc ggtgatggtc gtcggtctcg
      901 tggccgaggg cccgcatttg gcgacctacg cctggcgcgg cccgctgttc gtctcgctgt
      961 cgattctttc atttgccgtc acgatccgcc cgctgggtct ggtgctgtcg gcatttgcaa
     1021 gcttcatgat ctcagcgctg ggcacgccgg aaacccggtg gaaggaaacc atcatcgtcg
     1081 gaatttgcct gacgatcggc tgcagcctgc tcttcccgta tgcgctgggg ttgccgatgc
     1141 agctgttccc gcgtttcctg gtgcagtgag ggtgcgatgg ttgaactttt ccataatcta
     1201 gccctcggct tcggcgtcgc attttcgccg atcaatcttc tgctgtgtct gatcggcgcg
     1261 ctcgtcggta cgctggtcgg cgtgctgccg ggcatcggca ccatcgccac cgtcgcgatg
     1321 ctgctgccga ttactttcgg tctgccgccg gtcggcgcgc tgatcatgct cgccggcatt
     1381 tactacggcg cgcaatatgg cggctcgacc acctcgatcc tggtcaatat tccgggcgag
     1441 gcgacatcgg tggtgacgac gctcgatggt ttccagatgg cgaaacaggg ccgggccggt
     1501 ccggcgctcg cgatcgccgc gatcggatcg ttcttcgccg gctgcgtcgc gaccgttctg
     1561 atcgctgtgc tcggcactcc cttgaccaaa ctcgcgctgg cgtttgggcc cgccgaatat
     1621 ttctcgctga tggtgctggg cctgatcttc gcaacggtgc tcgcgaaagg ctcggtcctg
     1681 aaggccattg cgatgatcgt gttcgggttg ctgctgtcga tggtcgggtc cgacatcgag
     1741 accggcgcat cgcgcatggc gtttaatgtt cctgaactcg ccgatggtct cggctttgcc
     1801 acggtggcga tgggcgtatt cggattcgcg gagatcattc gcaaccttga cgccggcgcc
     1861 gagatggacc gccatctcgt gcagcagaag atcaccgggc tgatgccgac caagaaggat
     1921 ctgatcgctt cggcgccggc tatcgtgcgc ggcacgatcc tggggtcaat tctcggcatc
     1981 ctgccgggcg gcggcgcggt gattgcctcg tttgccgcct ataccttcga gaagaagatc
     2041 gccaaagatc cgtcgcggtt cggccgcggc gcgatcgaag gcgtcgcggc gccggaaagc
     2101 gccaataacg cggcggcgca gacctcattc atcccgctat tgacgctcgg cattccgccc
     2161 aacgccgtga tggcgctgat ggtcggcgcg atgaccattc acggcatcgt gccgggaccg
     2221 caggtgatgc agaagcagcc cgagctggtg tggggcatga tcgcctcaat gtggatcggc
     2281 aatctgatgc tgatcatcat caacctgccg ctggtcggca tctgggtgcg gttgctgcgg
     2341 gtaccgtatc gcctgatgtt cccgtcgatc gtgatctttt gcgcgatcgg catctactcc
     2401 atcaacaacg ctccggtgga cgtcatcctc gccggcgcgt tcggattgct cggctattgg
     2461 ctgatcaagc atgatttcga accggcgccg ttgctgctcg ggatggtgct cgggccgttg
     2521 atggaagaga acctgcgccg ggcgctgttg atctcgcgcg gcgatgcgac ggtgttcttc
     2581 acccgcccgc tttcggcggc gctgatggca atggccgcct tcctgctggt gctggcgttg
     2641 ctgccgtccc tgcgaaaaaa gcgcgacgag gtgttcgtgg agtccgagac ctgagcggct
     2701 tcgggacggc cgtgaattga gggcggaacg cgggttccgc ccttgccgtt tatgagccga
     2761 atgttcattt ttccgggtat aatcgcagca atcggccgaa tcgccgtgtc gattggccgg
     2821 tcgggccgct aaattcgccg tctcgagggc ctctaacgta aagccccgca acgaaagcca
     2881 tgaaccaata tcacgatctg cttgaacgga ttctctcgga cggcgcggaa aaacacgacc
     2941 gcaccggcac cggcacgttg tcgatattcg gccatcagat gcgcttcaat ctggccgccg
     3001 gctttccgat gctgaccacc aagaagctgc cgctgaaatc gatcgtgcac gaattgctgt
     3061 ggtttctcaa aggcgatacc aacatcaaat atctcaggga tcacggcgtt tcgatctggg
     3121 acgaatgggc cgacgccaat ggcgatctcg gaccggtcta tgggtcgcaa tggcgatcct
     3181 ggccggcgcc ggacggacgc agcatcgacc agatctccaa tgtcatcgcc atgatccgcc
     3241 gcaatccgga ttcgcggcgc ctgatcgtca gcgcgtggaa tccggccgat gtcgacaaga
     3301 tggcgctgcc gccatgccat tgcctgtttc agttctacgt cgcgaacggc aaattgtcgt
     3361 gccagctcta tcagcgttcg gcggacgtgt ttctcggcgt gcccttcaac atcgcgtcct
     3421 atgcgttgct gacgctgatg gtggcgcagg tgacggacct caagcccggc gacttcgtcc
     3481 atagtctggg cgacgctcac ctctattcga accatctcga gcaggcgcgg ctgcaactga
     3541 cgcgtccgac gcggccgttg ccgaccatga agatcaatcc tgaggtgaag gatatcttcg
     3601 cgttccgcta tgaagacttc gtgctcgagg gctacgaccc gcacccgcat atcaaggccg
     3661 aggtcgcggt gtgagtgaag cgtcattccg gggcgcgtcg aagacgcgaa cccggaatct
     3721 cgagattccg ggtctggtcc ttgcggacca tcccggaatg acgagactat ctggcgtcgg
     3781 aattggaaag atacgcgcgg catatgtcat ttcctgaaat cgtccttgtt gtcgcggtcg
     3841 ccgacaatgg cgtgatcggc tctcgcggcg ccctcccatg gcggctgaaa tccgacattc
     3901 aacgcttcaa ggcgatcaca acaggcaagc cggtggtgat ggggcgcaag acgtttgctt
     3961 cgattggccg gccgcttccc ggacgaacca atatcgtggt cacgcacgat gcaaattttc
     4021 gcgctcgtgg agcggtggtg gtgacgtcgt tcgccggcgc cctcgcggtc gctttgggcg
     4081 atgcgctgcg gcgttgggcg actgacattg ccgtgatcgg cggtgccgaa atctacgaac
     4141 aatggctgga tatcgccgac cgcctggaag tcaccgaagt tcacgcccgg cccgagggcg
     4201 acacgcattt tgccgcaatc gatgcggcca actgggaaga ggtggcgcgc gtgcgcaatc
     4261 cggccggccc ggacgacagc gcggacttct cctatgtgac atatcgccgg cgaaggtcgc
     4321 attaactccg ttcggcaatg tttacattga caattattgg ttcaggcata gctgcttcga
     4381 gccaacaagc cgcgttgtaa gggccggggc ggtcccctat aaagccgtgc agatgttgcg
     4441 aggccgggcg ttttgccctc aagggccccg cggaggagac tgtccatgcc gtggaagaat
     4501 caaggcggag gcccgtgggg ctcgggtccg aaagggccgt ggggctcggg accgcaaccg
     4561 gttgggccaa ggccgcccga tcttgaagac cttctgcgcc gcgctcagga ccggctgcag
     4621 caattgctgc cgggcggcta cttcagcggc ttgggcattg cgctgcttct gatcggcgcg
     4681 ttggcgatct ggggactgtc cggatttttc cgcgtccagt ccgaagagct cggcgtcgtg
     4741 ctgcgctttg gcaagcatgt gcgtacggtg cagccgggcc tgaactatca cctgccgtat
     4801 ccgat
//
LOCUS       DATNOI010000381         2924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1827199,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000381 DATNOI010000000
VERSION     DATNOI010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827199"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /gene="chrA"
                     /locus_tag="VN968_15150"
     CDS             complement(<1..1048)
                     /gene="chrA"
                     /locus_tag="VN968_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HXL30608.1"
                     /translation="MTDIPAGQKVADQEIGHGITFGEAFRVWLRVAVLSFGGPAGQIA
                     VMHRILVEEKNWISESRFLHALNYCMLLPGPEAQQLATYIGWLLHRTAGGIMAGGLFI
                     LPGVIAIMGLSYIYAAFGKVGFIEALFFGLKAAVLAIVIEAVVRVGKRALRNRIMIAL
                     AAIAFIAIFFFAVPFPVIIIGAGVIGYIGAKIGRPEFAAVPHGGGKDAAAIDSMLGEE
                     LPEHVRPSVARALKVSAVWLLLWLVPVAALLIAFGEANVFSQIALFFSKMALVTFGGA
                     YAVLAYVAQQAVEHYHWVSAREMLDGLGMAETTPGPLIMVLQFVGFMAAYRDPGTLSP
                     MLAGTLGGLLATWVT"
     gene            complement(1305..2135)
                     /locus_tag="VN968_15155"
     CDS             complement(1305..2135)
                     /locus_tag="VN968_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HXL30609.1"
                     /translation="MSSPTTISPDKLARLIGTANRPALIDVRTDEDFAADPRLIPGAV
                     RRNHQAAPDWGKEFSGHSAIVVCLRGEKLAQGTAAWLRYVKVEAEALEGGFEGWKAAK
                     LPLVPASKLPARDAQGRTVWVTRARPKIDRIACPWLIRRFIDPNAVFLFVAPSEVVAV
                     GERFNAVPFDIENVFWSHRGELCTFDVMIEEFGLATPPLLRLATMVRGADTARLDLSP
                     EAPGLLAASLGLSRMYDDDLEQLEAGMTLYDAFYRWCRDATGETHNWPTAKPVAKPAR
                     "
     gene            complement(2325..>2924)
                     /locus_tag="VN968_15160"
     CDS             complement(2325..>2924)
                     /locus_tag="VN968_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXL30610.1"
                     /translation="QTATLIIAFGLIVQGYSVWKLRRALDWKRLWPFVTGAALGVPIG
                     VSILTWANPANVRAGVGAFLVLYSLYALLRPTIAPVKTAGAAVDAGVGFLNGVIGGIT
                     GLAGILVTIWCGLRGWPKDVQRTVFQPVAVATFLMSALWIGARGAITADTVKLFLIGL
                     PALFAGTWLGLKLYGRLDEAAFRKVVLVLLLASGVVLIV"
BASE COUNT          586 a          956 c          878 g          504 t
ORIGIN      
        1 aggtgaccca ggtcgccagc aacccgccga gtgtgcccgc cagcatcggc gacagcgtgc
       61 cggggtcgcg gtaggcggcc atgaagccga cgaactgcag caccatgatc agcgggcccg
      121 gcgtggtctc ggccatcccc aagccatcga gcatttcgcg cgcgctcacc cagtgataat
      181 gctcgaccgc ctgttgcgcg acataggcca gcaccgcata ggcgccgccg aacgtcacga
      241 gcgccatctt gctgaagaac aatgcgatct ggctgaacac gttggcttcg ccgaacgcga
      301 tcaagagcgc cgcgaccgga accagccaca gcaatagcca caccgcgctg actttcagcg
      361 cgcgcgccac gctcgggcgg acatgctcgg gcaattcctc gccgagcatg ctgtcaatcg
      421 ccgcagcatc tttgccgccg ccgtgcggca cggcggcaaa ttccggccgg ccgattttgg
      481 cgccaatata tccgatcacg cctgcgccga tgataatgac cgggaaggga acggcgaaaa
      541 agaagatcgc gatgaaggca atggctgcca gcgcgatcat gatccggttg cgcagcgcgc
      601 gttttcccac gcggaccacg gcctcgatca cgatcgccag caccgccgcc ttcaatccaa
      661 agaacagcgc ctcgatgaat ccgaccttgc cgaaggcggc atagatatag ctcaagccca
      721 tgatggcgat gacgccgggg agaatgaaca ggccgccggc catgatgccg cccgcggtcc
      781 gatgcaacag ccagccaata taggtcgcaa gctgctgcgc ctcggggccc gggagcagca
      841 tgcagtagtt cagcgcgtgc agaaaacggc tctcggaaat ccagttcttc tcctcgacca
      901 ggatgcggtg catcaccgcg atctgccctg ccgggccgcc aaagctcagc acggcaaccc
      961 gcagccagac tcggaaggct tcgccgaatg taattccatg gccgatttct tggtcggcga
     1021 ctttttggcc tgctggaatg tccgtcattt caagcccgtt gttcctgaca ttggagatga
     1081 catgtgcggc tatttctttc ggcacgactt catgcacatc tgatattcgg ttttgcaggt
     1141 cggaccggca tagttcttca tacaggcccg ctgctgatcg ctgcatacct tgcaactgtt
     1201 cgaggattgc gcaattgcta ggttgttcat gccgcacaag caaagtaacg ccagcaccat
     1261 gacgacataa gttcgcatcc gaattcccct tgatgctatg ggcattaccg ggccggcttc
     1321 gcgactggtt ttgccgtcgg ccagttatgg gtctcgccgg tcgcatcccg gcaccagcga
     1381 tagaacgcgt cgtaaagcgt catgccggcc tcgagttgct cgagatcgtc gtcatacatc
     1441 cgcgacaacc ccagcgaggc cgccagcagc ccgggcgctt ccggactgag atcgagccgt
     1501 gcggtatcgg cgcccctcac catcgtcgcc agcctcagca gcggcggcgt cgccaacccg
     1561 aactcctcga tcatcacgtc gaatgtgcag agctcgccgc gatggctcca gaacacgttc
     1621 tcgatatcga agggaacggc gttgaagcgc tcgccgaccg cgacgacctc ggaaggcgcc
     1681 acgaacaaga acaccgcgtt cggatcgata aaccgcctga tcagccaggg gcaggcgatg
     1741 cggtcgatct tcggccgtgc gcgggtcacc cagacggtgc gcccctgcgc gtcgcgcgcc
     1801 ggcagctttg aggccggcac cagcggaagt ttggcggcct tccagccctc gaagccgccc
     1861 tcgagcgctt cggcttccac cttgacatat ctcagccagg ctgcggttcc ctgggcgagc
     1921 ttctcgccgc gcaggcagac cacgatggcc gaatggccgg agaattcctt gccccagtcg
     1981 ggggccgcct gatggttacg tctgacagca ccgggaatta gccgcgggtc ggcggcaaag
     2041 tcctcgtcgg tgcgcacgtc gatcagggca ggcctattcg ccgtgccgat cagtcgtgcc
     2101 agtttatcgg gagatatcgt tgttggggat gacatggatg cgccctcgta agaacaaacg
     2161 ggacgcgata cttgggcatg tcgcctcgtg gggggatcgc agaaccccca tggttcgcat
     2221 tacagcgaaa ccggtgcggc ctgtcaatgt gcccatccac cctatagccc cgccgcgacc
     2281 gcatcgaact tgttttcacg taatttttat cagcgcagag gagtttagac gatcagcacg
     2341 acccccgagg ccagcagaag caccagcacg actttgcgga acgcagcctc gtcgaggcgc
     2401 ccgtacagtt tcagaccaag ccaggtgccg gcgaacaacg cgggcagccc gatcaagaac
     2461 aatttgacgg tgtccgcagt gatcgcaccc cttgctccaa tccacagcgc gctcatcagg
     2521 aaggtcgcca ccgccaccgg ctgaaacacc gtgcgctgca cgtccttggg ccagccgcgc
     2581 aggccgcacc agatggtgac gaggattccg gcaagcccgg tgatgccgcc gatcacgccg
     2641 ttgaggaatc cgacgccggc gtccaccgcg gcaccggctg tcttgaccgg agcgatggtg
     2701 ggacgaagca gggcatagag gctgtagagg accaggaacg cgccgacgcc cgcccgcaca
     2761 tttgcgggat ttgcccaggt caggatgctg acgccgatgg gtacgccgag cgccgcgccg
     2821 gttacaaacg gccaaagcct tttccagtcc agcgcgcggc gcagtttcca gaccgaatag
     2881 ccctgcacga tcaggccgaa cgcgatgatc agcgtggcgg tctg
//
LOCUS       DATNOI010000382         4123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2365589,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000382 DATNOI010000000
VERSION     DATNOI010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2365589"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VN968_15165"
     CDS             <1..521
                     /locus_tag="VN968_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL30611.1"
                     /translation="GRGMLYVPFLNYADGNLDAVREMLRDPHSVPGLSDGGAHCGIIC
                     DASFPTYLLTHWTRDRTRGEKLSIPFVVAAQSRKTALSVGLYDRGVIATGYMGDVNVI
                     DYDGLHLHPPKVHYDLPVGGRRLLQQVDGYDATIVSGVVTQRGGAATGARPGRLVRGA
                     QGSAPRIEAAAS"
     gene            complement(539..1810)
                     /locus_tag="VN968_15170"
     CDS             complement(539..1810)
                     /locus_tag="VN968_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL30612.1"
                     /translation="MTTQPTPKGAWRITFLLFLFMLVNFADKIVVGLAGVPIMTELKL
                     EPEQFGLLGSSFFFLFSIAAIVVGFIVNRIATRWVLLALALIWALAQFPMVGTVSFST
                     LLICRVILGAGEGPAFSVAAHAVYKWFPDEKRTLPTAILSQGSAFGVILAVPALNWVI
                     VNHSWHYAFGALGVVGLMWVVAWLFLGKEGPLVNTAAMAAAETRIPYLQLLTSRTFIG
                     CCAATFGAYWALSLGLTWFTPFIVKGLGFSQSEAGWVSILPWVFGAIIVLLTGWISQV
                     WMARGMTTRGARGVLGSVPLIVGGLILSMLPYVDGAGLQVAFLVVGYGLCGSIFVVCP
                     PMLGEFTPVSQRGAVISINGAIYTLAGILAPSVMGSVIQRAAVLLDGYLTGFTINAVI
                     MIASGLLGLLLLWPNTERARLIGELPQPKFA"
     gene            2061..2834
                     /locus_tag="VN968_15175"
     CDS             2061..2834
                     /locus_tag="VN968_15175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXL30613.1"
                     /translation="MVISIHRHARAVGMLAMLSMFAAWNMVSATAQDAKAPDYEIIVA
                     SPDRSDADRQADQRRQPAKMLAFAGVKAGMKVLDMEANAGYSTELLARTVGPTGVVYA
                     QDSAAVIERFVKDKFDIRAQKPAMKNVVHVVRNFDDPVPPEVGGLDLITFFFAYHDVT
                     YMELDRAAMNQKMFAALKPGGFLIIADHSARPGDGTTVAKTLHRIEESTLRQEIEASG
                     FKLAGEADFPRHPEDPRDAAVFRPQIPTDEFVLKYQKPL"
     gene            2875..3528
                     /locus_tag="VN968_15180"
     CDS             2875..3528
                     /locus_tag="VN968_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30614.1"
                     /translation="MLSVTNLAKTYRSAGEQIEVLRGVNLSIAAGERVALTGESGSGK
                     STLLHLIAGLDNADGGEIRLADAPVSGLSDAGRAALRRDRLGLVFQQFNLIPSLNVED
                     NLVFQSRIAGRHDAAWHAELVERLGLGGLLKRYPEQLSGGQQQRVAIGRALAVKPLLL
                     LADEPTGNLDEATADDVLALARDLVARTGCGFLMVTHSARLAATLDRQINLHAGLVA"
     gene            3525..>4123
                     /locus_tag="VN968_15185"
     CDS             3525..>4123
                     /locus_tag="VN968_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL30615.1"
                     /translation="MSRVIWILAVLLSHWRRHPMQLTTLLIGLISATALWSGVQALNQ
                     QARSSYDRAAATFGGARTAALVGRDGKPFSQQLFVELRRAGWPVSPVLEGRVQIGGRS
                     FRLLGIEPVTLPAEVGNAPAIGRADLQSFVTPPGQTLVAPEALAELNLPEGATPPSSG
                     GTSLPPLRAQAQLVPGVLVVDIGIAQRLLNMPDQVSRLL"
BASE COUNT          798 a         1313 c         1304 g          708 t
ORIGIN      
        1 aaggccgcgg catgctctat gtgccgttcc tgaactacgc cgacggcaat ctcgatgccg
       61 tgcgcgagat gctgcgcgat ccgcattcgg tgccgggatt aagcgacggc ggcgcccatt
      121 gcggcattat ctgcgacgcc agcttcccga cctacctgct gacgcattgg acccgtgacc
      181 gcacccgcgg cgaaaagctg tcgatcccgt tcgtggtggc ggcgcagtcg cgcaagacgg
      241 cactttcggt cgggctctat gatcgcggcg tcatcgcgac gggctacatg ggcgatgtca
      301 acgtcatcga ctacgacggc cttcatctgc atccgccgaa ggtgcattac gatcttccgg
      361 tgggtggccg ccgcctgctg caacaggtcg atggctatga cgccaccatc gtctcaggcg
      421 tggtgacgca acggggaggt gctgccaccg gagcgcgtcc gggccggctg gtgcgcggcg
      481 cccagggcag cgcacccagg atcgaagccg cggcttctta gccggcggct cccgatcatc
      541 acgcgaactt cggttgcggc agctcgccga tcagacgcgc gcgctcggta ttgggccaga
      601 gcagcaacag gccgagcaat cccgacgcga tcatgatgac ggcgttgatc gtgaagcccg
      661 tcaagtaacc gtcgagcagc accgccgcgc gctggatgac gctgcccatg actgatggcg
      721 ccaggattcc ggcgagggta tagatcgcgc cattgattga gatcaccgca ccgcgctgcg
      781 ataccggcgt gaactccccg agcatcggcg gacagaccac aaagatcgat ccgcaaagtc
      841 cgtaccccac caccagaaag gcgacctgca agcccgcgcc atcgacatac gggagcatgg
      901 acaggatcag cccgccgacg atcagaggga ccgagccaag cacgccgcgc gcgccgcggg
      961 tggtcatgcc gcgtgccatc cacacctgcg aaatccaccc cgtgagaagc acgataatgg
     1021 caccgaacac ccatggcagg atcgaaaccc agccggcctc actttgcgag aagcccaacc
     1081 ctttcacgat gaagggcgtg aaccaggtga ggccaagcga cagcgcccaa taggcgccga
     1141 aagttgcggc acagcagccg atgaaggttc gcgaagtcaa aagctgcaaa tagggaatgc
     1201 gggtttcagc cgccgccatg gctgcggtat tcaccagcgg cccttccttg cccaggaaca
     1261 gccacgccac gacccacatc aggccgacga cgccgagcgc gccgaatgca taatgccagg
     1321 aatgattgac gatcacccag ttcagcgccg gcaccgcgag gatcacgccg aacgccgagc
     1381 cctgcgacag gatggctgtc ggcagcgttc gcttctcgtc gggaaaccat ttgtagaccg
     1441 catgagccgc caccgaaaac gccgggccct cgccggcgcc gaggatcacg cggcaaatca
     1501 gcagcgtcga gaaactgacc gtgcccacca tcggaaattg cgccagcgcc caaatcaacg
     1561 ccagcgcaag gaggacccag cgcgtggcga tgcggttgac gatgaagccg acaacaatgg
     1621 ccgcgatcga aaacaggaag aagaacgagg agccgagcaa tccgaactgt tccggctcga
     1681 gcttcaactc ggtcatgatc gggacgccgg cgaggccgac cacgatcttg tccgcgaaat
     1741 tcaccagcat gaagagaaac aggagaaacg tgattctcca ggcccctttt ggcgttggtt
     1801 gcgtggtcat gatcgctcct cgaaaatcgt cttctagccc tgttgattgg atcggtccaa
     1861 tgctacccat tgggttaccg acaatgcaat tggacatttt gcccggatgg cgtgcactgc
     1921 aacccgcctc gcggatactc ccgacgcggt ttcggaagcg tcccacagcg gcatcacgat
     1981 cgcgtcgccg tcacgattga gaccgtgatc tgtcgccatg gtcttggtat gttgggcgac
     2041 aaccaagggg attttcaaat atggtcattt ccatccatcg ccatgcgcgc gccgttggca
     2101 tgctggccat gctttcgatg ttcgcggcat ggaatatggt atcggcgacg gcgcaggacg
     2161 ccaaggcgcc ggactatgag atcatcgtgg cctcgcccga ccgaagcgac gccgaccggc
     2221 aagccgatca gcgccggcag cccgccaaga tgctcgcctt cgccggcgtc aaggcaggca
     2281 tgaaggtgct ggatatggag gcgaacgctg gctacagcac ggagctgctg gcgcgcaccg
     2341 tcgggccgac tggcgtggtc tacgcgcagg attccgccgc cgtcatcgag cggttcgtga
     2401 aagacaagtt cgacatccgc gcccagaaac ccgccatgaa gaacgtcgtg catgtcgtga
     2461 ggaatttcga cgatccggtc ccgcccgagg tcggcggact cgacttgatc acatttttct
     2521 tcgcctatca cgacgtgacc tacatggagc tcgatcgtgc cgcgatgaac cagaagatgt
     2581 tcgccgcact gaagcccggc ggctttctga tcatcgccga ccattccgcc cgtcccggcg
     2641 atggcacgac ggttgccaag accctgcatc ggatcgagga aagtacgctg cgccaggaga
     2701 tcgaggcgtc cggtttcaag ctcgcaggcg aagctgattt cccgcgccat cccgaggatc
     2761 cgcgggacgc ggcggtgttc cgtccgcaga taccgaccga tgagttcgtg ctgaaatatc
     2821 agaagccgtt gtgaggcatc gcgtacgctt ggcatgacaa ccgaccatca gaacgtgctg
     2881 agcgttacca atctcgccaa gacctatcgc tcggccggag agcagatcga ggtgctgcgc
     2941 ggggtgaacc tgagtatcgc ggcaggcgag cgcgtcgcgc tgacagggga atccggcagc
     3001 ggcaagagca ccctgctgca tctgatcgcg ggactggaca atgctgacgg tggcgaaatc
     3061 aggttggccg acgcgcctgt gtctggtcta tccgatgccg gccgggcggc attgcggcgc
     3121 gatcggctcg gtcttgtttt tcaacagttc aacctgatcc cgagcctgaa tgtcgaggac
     3181 aatctcgtgt tccagtctcg catcgccggc cgtcacgatg ccgcctggca tgccgaactg
     3241 gtcgagcggc tggggcttgg cggtttgctg aagcgctatc cggagcaatt gtccggcggc
     3301 cagcagcaga gggtcgcgat cggccgcgcg ctggcggtga agccgcttct gcttctcgcc
     3361 gacgagccca ccggcaatct cgatgaggcg acggcggatg acgttcttgc gcttgcgcgc
     3421 gatctcgttg cccgaaccgg ctgcggtttc ctgatggtaa cccacagcgc gcggttggcg
     3481 gccacgctcg accggcagat caatctccat gcgggacttg tcgcatgagc cgcgtcatct
     3541 ggattctcgc ggttctgttg agccattggc ggcggcatcc gatgcagctt acgacattgc
     3601 tgatcggact gatttccgcg accgcgctgt ggagcggcgt gcaggcgctg aaccagcagg
     3661 ctcgctcaag ttacgaccgc gccgccgcga cgttcggcgg cgccagaacg gcggctctgg
     3721 tcggccgcga cggcaagcct ttctcgcagc agctcttcgt cgagctgcgc cgcgccggct
     3781 ggccggtgtc gcccgtcctc gaaggccgtg tccagatcgg cggacggtcg ttccggctgc
     3841 tgggcatcga gcccgtcacg ctgcccgcgg aagtaggcaa tgcgccggcg atcggaagag
     3901 cagacttgca atcgttcgtc acgccgccgg gacaaacgct ggtggcgccc gaagcgcttg
     3961 ccgaattgaa cctgccggaa ggcgcaacac cgccttcgag cggcggcacg tcgctgccgc
     4021 cgctgcgcgc gcaggcgcaa cttgtgccgg gcgtgctggt ggtcgatatc ggcatcgcgc
     4081 agcggctgct gaacatgccg gaccaggttt cccgtctttt gat
//
LOCUS       DATNOI010000383         5993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_251425,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000383 DATNOI010000000
VERSION     DATNOI010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251425"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VN968_15190"
     CDS             <1..956
                     /locus_tag="VN968_15190"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HXL30616.1"
                     /translation="GEVKLTDEYRVSENAWRKGGAPSGGSTMFAAIHSKIPVDDLLHG
                     AIIQSGNDACMVLAEGIAGNERTFAEMMTKRARELGLSQSTFANSNGLPDPGNKMTVR
                     ELAKLARHIIQTYPDFYKLFGEREFTWNKIRQQNRNPLLTSLEGSDGLKTGYTKEGGY
                     GMVGSVVQNGLRLIVVVNGLDDPDDRASEAKKMLEWGFRNFEARTLFAAQQPVGYAKV
                     FGGESRSVKLASPEPIKVMVHKNGTDKLIARVVYSGPVRAPIQSGQRVGVVRVWRGAN
                     IAVDAPIYAAESVGRGSTIRRAIDGASELVIGMFRAGAEKL"
     gene            959..1651
                     /gene="tmk"
                     /locus_tag="VN968_15195"
     CDS             959..1651
                     /gene="tmk"
                     /locus_tag="VN968_15195"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HXL30617.1"
                     /translation="MAEAAVKRTAGRGRFITFEGGEGSGKSTQIKILAERLGAAKLRT
                     IVTREPGGSPGAEIIRHLVLSGMGKLLGPEAETLLFAAARDDHVRTVIRPALNQGIWV
                     LCDRFSDSTRAYQGSLGKVSPDVLNAMNRVTIGDLKPDLTIILDVPVEVGMQRSAKRR
                     GTGTPDRFEAEDLKFHQDLRDAYRQIAAAEPQRCVLIEANADANTVTARIWATLRDRF
                     FAAGADNVATSA"
     gene            1648..2691
                     /locus_tag="VN968_15200"
     CDS             1648..2691
                     /locus_tag="VN968_15200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HXL30618.1"
                     /translation="MSARQVEPQIAAVHPRETSILAGHREAEAALLNAYHSGRIPHAW
                     LIGGPQGIGKATLAYRMARFVLAHRNPAASDVQSAETLWVDPSEPVARHVAAGAHGGL
                     LALERTLNDRGVMRTVITVDETRETISFFGSTAAVEGWRVCIVDTVDELNPNAANALL
                     KILEEPPRQSLFLLVSHAPARVLPTIQSRCRKLSLRPLATDDVIRAAAKAANLATDDQ
                     ALAEAAAAAEGSVGRALTLLGGDALKLQQRTAALLATLPRVDPRELHALGDALGPSDR
                     VALAAFIDSVDRWVSERLRSDANANANLPRLARLAEVWENINRAARDTAEYNLERKPL
                     VFSVFGLLAEAIG"
     gene            2710..4320
                     /gene="metG"
                     /locus_tag="VN968_15205"
     CDS             2710..4320
                     /gene="metG"
                     /locus_tag="VN968_15205"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HXL30619.1"
                     /translation="MYHHTSVEERKAFAIMANATNLHRERYYITTAIAYPNGVPHIGH
                     AYEAIATDALARFARLDGKDVFFLTGTDEHGLKMMQTAEAESLPTLEVATRNALRFKE
                     MDQRLNVSFDRFIRTTEEQHHRSSQEIWKRIADNGDIYLDNYAGWYSVRDEAYYAEEE
                     TVLGEDNVRRGPQGTPVEWVEEKSYFFKLSAYQHKLLALYESQPDFIGPDSRRNEVIS
                     FVKSGLRDLSISRTTFDWGVKVPGDPEHVMYVWLDALTNYITGVGFPDETDPNWRYWP
                     ADVHIIGKDIIRFHAVYWPAFLMSAGIPVQKRVYAHGFLFNKGEKMSKSVGNVVDPFN
                     LANQYGVDQMRYFFLREVPFGQDGNYNHEVIVARINADLANDLGNLAQRSLSMIAKQY
                     DGVLPEPGAFTANDKAILAHADGMVGLARAAMATQQIHQALNAVWAVVAEANRYFAGE
                     APWALAKSDPQRQKTVLYVTSEVVRQIAILAQPVMPESSAKLLDSLGIPQDARDFAAL
                     GGAARIKPGTVLPPPAPVFPRYVEPSAA"
     gene            4336..5115
                     /locus_tag="VN968_15210"
     CDS             4336..5115
                     /locus_tag="VN968_15210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXL30620.1"
                     /translation="MLVDSHCHLDFPDFADDLDAIVARAAAAGIGRIVTISTRIRRLG
                     ELLAITERFPNVYCSVGTHPHHADEEDGIPADELIGLTRHPKVVALGEAGLDYFYQHG
                     SREAQERGFRAHIAAARATGLPLVVHTRDADDDCGRILEDEIAKGPFRAVLHCYTGGR
                     ELAMKAISLGLSISFTGILTFRKSQSLRDLAAELPADRIMVETDAPYLAPGKFRGKRN
                     EPAYVVEIARVLAETRGVSLEEISHQTTQNFFRLFSKVPAA"
     gene            5112..5912
                     /locus_tag="VN968_15215"
     CDS             5112..5912
                     /locus_tag="VN968_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL30621.1"
                     /translation="MTLTLTILGCGSSAGVPRPALGWGACDPSNLKNRRRRCSLLVER
                     TGDRGVTRILIDTSPDLREQLIDAKVDHIDAVFLTHEHADQTHGIDDLRSVVLHQRRR
                     IPVYLSQSTADQILLRFAYCFVSPPESDYPPILNSHLIEAGQSRAIEGKGGPVTLTAF
                     MLRHGSVPALGYRVGDAAYTPDVNDIPRESWPHLENLDLWIIDGLRYTGHPSHFSVND
                     ALSWIDRFKPRRAVITNMHSDLDYEVLRQSLPPEVIPAYDGMRLSAQP"
BASE COUNT         1160 a         1860 c         1886 g         1087 t
ORIGIN      
        1 agggcgaggt caaactgacg gacgaatacc gggtcagcga gaatgcctgg cgcaagggcg
       61 gcgccccctc gggcggatcg accatgttcg ccgccatcca tagcaagatt ccggtggacg
      121 atctcctgca cggcgccatc attcagagcg ggaacgatgc ctgcatggtg ctggcggagg
      181 gcatcgccgg caatgagcgc acctttgccg agatgatgac caagcgcgcg cgtgagctcg
      241 ggttgtcgca gtcgaccttc gccaattcaa atggtcttcc cgatcccggc aacaagatga
      301 ccgtacgtga actggcaaaa ctcgctcgcc acatcatcca gacctatccc gatttctaca
      361 aactatttgg cgagcgcgaa tttacctgga acaagatcag gcagcagaac cgcaatccgc
      421 tgctgacctc gttggaaggc agtgacgggc tcaagaccgg atataccaag gagggcggct
      481 acggcatggt cggttccgtc gtgcagaatg ggctgcggct gatcgtcgtg gtcaacggtc
      541 tcgacgatcc cgacgatcgc gcctccgaag ccaagaaaat gctcgaatgg ggatttcgca
      601 atttcgaagc gcgcaccctg ttcgcggcac agcagccggt cggctacgcc aaggtgtttg
      661 gcggagagag ccggtcggtc aagcttgcga gccctgagcc gatcaaggtg atggtgcaca
      721 agaacgggac cgataaattg atcgcgcgcg tggtctatag tgggccggta cgtgcgccaa
      781 tccagtccgg tcagagggtc ggtgtcgtca gggtatggcg cggcgccaac atcgccgtgg
      841 acgcgccgat ttatgcggcg gaatcggtcg gccggggatc gacgatccgg cgggcgatcg
      901 atggcgcgag cgagctcgtc atcggaatgt ttcgcgccgg cgccgagaaa ctctgatgat
      961 ggccgaggca gcggtcaaaa gaactgcggg acgcggacga ttcatcacct tcgagggtgg
     1021 cgagggatcc ggcaaatcca cgcagatcaa gatcctcgcg gagcgtcttg gcgcagcaaa
     1081 gctgcggacc atcgtcacgc gcgagcccgg cggctcgccg ggagccgaaa ttatcaggca
     1141 tctcgtattg tccggaatgg gcaagctgtt aggccccgaa gcggagacgc tgttgtttgc
     1201 agcggcgcgt gacgatcacg tccgcaccgt catcaggccg gcgctcaatc agggaatatg
     1261 ggtgctgtgc gaccgctttt cggattcgac gcgtgcctat cagggcagcc tcggcaaggt
     1321 gtcgcccgac gttttgaatg cgatgaaccg ggtaacgata ggcgacctca agcccgatct
     1381 gaccatcatt ctcgatgttc cggtggaagt cggcatgcag cgctcggcca agcgccgcgg
     1441 caccggcaca cccgaccggt tcgaggcgga agacctcaag tttcatcagg acttgcgtga
     1501 cgcctaccgg cagatcgccg ctgctgagcc gcagcgatgc gtgttgatcg aagcaaatgc
     1561 cgacgccaat acggttaccg ccaggatatg ggccacgttg cgcgaccgtt tcttcgcagc
     1621 cggcgcggat aacgtggcca cttcagcatg agcgcccgtc aggttgagcc gcagattgcg
     1681 gctgtccatc cgcgagagac cagcattttg gcagggcatc gcgaagccga agcggcattg
     1741 ctgaatgcct accacagcgg gcgcattccg catgcctggt tgatcggggg gccgcagggc
     1801 atcggcaagg cgacgctggc ctatcgaatg gcgcggtttg tgctcgcgca tcgcaatccc
     1861 gcggcctcgg acgtgcaaag tgccgagacg ctttgggtcg atccatccga accggtggcg
     1921 cgccatgtcg cggcgggcgc ccatggcggc ttgctcgcgc tcgaacggac gttgaacgac
     1981 aggggcgtga tgcgaaccgt gattacggtc gacgagaccc gggagacgat ttcgtttttc
     2041 ggctcgaccg cggcggtcga aggttggcgg gtctgcatcg tcgataccgt cgacgagctc
     2101 aatcccaacg cggccaacgc cctgctcaaa attctcgagg agccgccgcg gcaatcgctg
     2161 ttccttctcg tcagccatgc ccccgcgcgg gtgttgccga ccattcagtc gcgatgccgc
     2221 aaattgtcgt tgcggccgct cgcgactgac gacgtcatcc gcgccgcggc caaggcggca
     2281 aacctcgcga ccgacgatca ggcgctggcg gaagcggccg cagcagccga ggggagtgtt
     2341 gggcgcgccc tgacattgct cgggggcgat gccttgaaac ttcagcagcg gaccgcggcg
     2401 ctgctggcga ccctgccgcg ggttgatccg cgcgagcttc acgccctcgg cgacgcgctg
     2461 ggtcccagcg accgggtggc actggcggcc ttcatcgaca gcgtcgatcg ctgggtaagc
     2521 gaacgcctgc gcagcgacgc caatgccaat gcaaacctgc cccgccttgc acggctggcg
     2581 gaggtgtggg aaaacatcaa tcgcgccgcg cgcgataccg cagaatataa tctcgagcga
     2641 aaaccgctgg ttttctcggt gttcgggctc cttgcggagg cgatcggctg acaggtcggt
     2701 ttagccagtc tgtatcacca cacgtcggtt gaagagcgaa aggccttcgc aattatggcg
     2761 aacgcaacca atttgcaccg tgagcgctat tatatcacga ccgccatcgc ctatccgaac
     2821 ggcgtgccgc atatcggcca cgcctatgag gcgatcgcca ccgatgctct ggcgcgcttt
     2881 gcgcggctcg acggcaagga tgtcttcttt ctcaccggga ccgacgagca cggcctcaag
     2941 atgatgcaga ctgcggaagc cgaaagcctt ccgacattgg aagtcgcaac gcgcaacgcg
     3001 cttcgattca aggagatgga tcagcgcctc aatgtctcgt tcgaccggtt catccgcacc
     3061 acggaagagc aacatcatcg ctccagtcag gaaatatgga agcggatcgc cgacaacggc
     3121 gatatctatc tggataacta tgccggctgg tattcggtgc gcgacgaggc ctactacgcc
     3181 gaagaagaaa cggtgctggg cgaggacaat gtgaggcggg ggccgcaggg cacgccggtc
     3241 gaatgggtcg aggagaagag ttatttcttc aaattgtcgg cctatcagca caagctgctg
     3301 gcgctctatg agagccagcc ggatttcatc ggccctgact cgcgcaggaa cgaagttatc
     3361 agcttcgtca aaagcggatt gcgcgatctg tcgatttcgc ggaccacatt cgactggggc
     3421 gtcaaggtgc ccggcgatcc cgagcatgtc atgtatgtct ggctcgacgc cttgaccaac
     3481 tacatcaccg gcgtcggctt tccggatgaa accgatccga actggcgcta ctggccggcc
     3541 gatgtgcaca tcatcggcaa ggacatcatc cgctttcacg ccgtgtactg gccggcgttc
     3601 ctgatgtcgg cgggcattcc ggtgcagaag cgcgtctacg cccacggctt cctgttcaac
     3661 aagggcgaga agatgtcgaa atcggtcggc aacgtggtcg atcccttcaa tctcgcaaat
     3721 caatacggcg tcgatcagat gcgctacttc ttcctgcgtg aagtgccgtt cgggcaggac
     3781 ggcaactaca atcacgaagt cattgtcgcg cgcatcaatg ccgatcttgc caacgatctc
     3841 ggcaatctgg cgcaacgctc gctgtcgatg atagcaaagc agtatgacgg cgtgctgccg
     3901 gagccgggtg ccttcaccgc caacgacaag gcaatcctgg cacacgccga tggcatggtc
     3961 ggattggcgc gggccgcgat ggcgacccaa cagattcatc aagcgctcaa cgcggtctgg
     4021 gccgtcgttg cggaagccaa ccgctatttc gcgggcgagg cgccatgggc gctggcaaag
     4081 agcgatccgc aacgccagaa aaccgtgttg tacgtgacat cggaagtcgt ccggcagatc
     4141 gccattctgg cgcagcccgt catgccggag tcctcggcga agttgctgga cagtctcggc
     4201 atcccgcagg acgcgcgcga cttcgccgcg ctgggcgggg cagcgcgcat caagccgggc
     4261 acggtgttgc cgccgccggc gccggtgttt ccgcgttacg tcgaaccgtc ggcagcctga
     4321 aactgttgga atgcgatgct ggtcgatagc cactgccacc tggattttcc cgacttcgcc
     4381 gacgatctcg acgccatcgt ggcgcgggcg gctgccgcag ggatcgggcg catcgtcacc
     4441 atctcgacgc ggataaggcg cctcggcgag ctccttgcga tcacggagcg gtttccaaac
     4501 gtctattgct cggtcggcac ccatccgcac cacgccgatg aggaagacgg gatacccgcc
     4561 gacgaactga tcgggctgac gcggcatccc aaggtcgtgg cgctcggcga ggcggggctg
     4621 gattatttct accagcacgg ctcgcgagaa gcccaggagc gcggctttcg cgcgcacatc
     4681 gccgccgccc gcgcgaccgg cctgccgctg gtcgttcata cccgcgatgc cgacgatgac
     4741 tgcggccgca tcctcgaaga cgagatcgca aagggaccgt tccgcgccgt gctgcattgt
     4801 tacaccggcg ggcgggagct tgcgatgaaa gcaatctcgc tgggtctgtc gatttcattc
     4861 acgggcattc tgacgttcag gaaatcgcaa agcttgcgcg atctcgcggc cgagcttccg
     4921 gccgaccgca tcatggtcga gaccgacgcg ccgtatctcg cgcccggcaa gtttcgcggc
     4981 aagcgcaacg agccggctta cgtggtcgag atcgccaggg tgctggcgga gacgcggggc
     5041 gtctcgctcg aggagatatc gcatcagacc acacagaatt tcttccgcct gttctccaaa
     5101 gtgccggctg catgacgctg acgctcacaa ttctcggctg tggctcttcc gccggcgtcc
     5161 cgcgtcccgc gctcggctgg ggtgcgtgcg atcccagcaa tctgaaaaac cgccgacggc
     5221 gctgctcgct gctggtcgag cgcacggggg atcgtggagt cacgcgaatc ctgatcgata
     5281 cctcgcccga tctgcgcgag cagttgatcg acgccaaagt cgatcacatc gacgcggtgt
     5341 tcctgaccca tgagcacgcc gaccagaccc acggcatcga cgatctccgt tccgtcgtgc
     5401 tgcaccagcg caggcgcatt ccggtctatc tgagccagtc gaccgccgat cagatcctgc
     5461 tgcggttcgc ctattgtttc gtctcgccac cggaaagcga ctatccgccg atcctcaaca
     5521 gtcacctcat cgaagccggt caaagccgcg cgatcgaagg caagggcggt ccggtgacgc
     5581 ttacggcgtt tatgctgcgg cacggcagcg ttccggccct cggttatcgc gtcggtgatg
     5641 ccgcctatac gcccgatgtc aacgacatcc cgcgcgagag ctggcctcat ctggaaaatc
     5701 tcgatctctg gatcatcgac gggctgcgct ataccggcca tcccagccac ttcagcgtca
     5761 acgacgcgct gtcatggatc gaccgtttca agccgcgccg tgccgtcatc accaacatgc
     5821 attccgatct cgactacgag gtgctgcgcc aaagcctgcc gccggaggtg atcccggcct
     5881 atgacggcat gcggctgtcg gcgcagccat agcccgattc aattttcaaa cagccaggaa
     5941 cggatgcgca accgcgatct cgcggcgcga tgcgcccgag gtttgctaaa act
//
LOCUS       DATNOI010000384         8997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_109765,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000384 DATNOI010000000
VERSION     DATNOI010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8997
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109765"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VN968_15220"
     CDS             <1..211
                     /locus_tag="VN968_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HXL30622.1"
                     /translation="ILNAPQSGILGMHKIQDRPVAIGGKVEIRPMMYLALSYDHRVID
                     GKDAVTFLVRVKESLEDPARLVLDL"
     gene            297..1043
                     /locus_tag="VN968_15225"
     CDS             297..1043
                     /locus_tag="VN968_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL30623.1"
                     /translation="MTDRVAVITGGSRGIGRATAISAAARGFKVCVGYASNEAAARQV
                     VSAIEAKNGKAIALKCDVGNESDILALFKAADAFGTLGALVNNAGIVGATSRVDEMSA
                     ERIQRMMAVNVTGSILCAREAVRRMSTRHGGKGGVIVNLSSVASKLGSPNTYVDYAAS
                     KGAIDSFTIGLGYEVAGEGIRVAAIRPGLIDTEIHASGGEPDRAHRLSHMVPMKRVGT
                     ADEIANAIVWLMSDDASYVTSAILDVSGGR"
     gene            1079..2482
                     /gene="lpdA"
                     /locus_tag="VN968_15230"
     CDS             1079..2482
                     /gene="lpdA"
                     /locus_tag="VN968_15230"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXL30624.1"
                     /translation="MATYDLVVIGTGPGGYVCAIRGAQLGMKVAVVEKDAALGGTCLN
                     VGCMPSKALLYASEMFEEAAHSFAKMGVSVSAPKLDLPSMMKFKQQGIDGNVKGVEFL
                     MKKNKIDVISGKGRILGTGKVEVTGNDGKTQTVETKNIVIATGSDIAPLKGIEIDEKR
                     IVSSTGALSLDRVPERLLIVGAGVIGLELGSVWHRLGAQVTVVEFLDRILPGNDGEVA
                     KQFQRILEKQGFAFELSTKVTAVDTSGKTLIAQVEPAAGGKPEKLEADVVLVSIGRVP
                     YTEGLGLKEAGVVLDNRGRVQIDAHFATSVKGVYAIGDVVAGPMLAHKAEDEGVAVAE
                     ILAGQAGHVNYDVIPSVVYTTPEVSAVGKTEEELKQAGIAYTVGKFPFTANGRSKVNQ
                     TTEGFVKVLADAKTDRVLGVHIVGREAGEMIHEAAVLMEFGGSAEDLARTCHAHPTRS
                     EAIKEAALAVGKRSIHM"
     gene            2521..3051
                     /locus_tag="VN968_15235"
     CDS             2521..3051
                     /locus_tag="VN968_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30625.1"
                     /translation="MMRRLLQPVWVLLAVIFLIEAWLWDHLEPVVAWFVALIPLRAFK
                     AWLAERVDTLSPAMTLIVFIVPVVPLFPLKLVALWLLAHEYWMAAVLTFVFAKFVGVG
                     VAAFIFDVTRPKLLEMEWFEKLYEFVIALRAKAVALVDPIKLRIKEVLSGGGDGWSSR
                     LLRLIQRFRKSVHEAR"
     gene            complement(3071..3661)
                     /locus_tag="VN968_15240"
     CDS             complement(3071..3661)
                     /locus_tag="VN968_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HXL30626.1"
                     /translation="MSAHESMEHAESAEHASSGNKKIALLIAVIALFLAMSETLGKSA
                     QTEAISKNVEASNLWAFFQAKTIRRTVVQAVSEQARLSLGLMGDDAAKTALQKQIEEW
                     QKTAARYRSEPETGEGSEQLSERAKHAEHERDLALARYHHYELASAAFQIGIVLASAT
                     IITGIVALAWISGLLTLAGLAFTAIGLLAPHAVHVM"
     gene            complement(3759..4730)
                     /locus_tag="VN968_15245"
     CDS             complement(3759..4730)
                     /locus_tag="VN968_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HXL30627.1"
                     /translation="MTKSAPATQQIELDCADDGIAREMTRWLSHLRSERRLSPKTLEA
                     YARDVRQCLGFLGEHWGSRVTLSRLAALEASDVRAFMAMRRGHDIGGRSLMRALAGLR
                     SFARFLEQEGRGKVGALSAIRAPKVGKSLPKPIHMAAAKRLADADERAGETRDPWILA
                     RDAAVMALLYGSGLRISEALGLKRRDVPLPGAGDVLVVNGKGNKARMVPVLQNVLALV
                     QDYVAICPHPLPPQGPIFVGARGGPLKARIIQLTMERLRGALGLPDSATPHALRHSFA
                     THLLSRGGDLRAIQELLGHASLSTTQIYTGVDSERLLEVYKTTHPRA"
     gene            4902..7112
                     /locus_tag="VN968_15250"
     CDS             4902..7112
                     /locus_tag="VN968_15250"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXL30628.1"
                     /translation="MDHSIRASTSSASSARVVDVLVPVALNQTYSYRVPRGMDVAPGD
                     VVCVPLGPREAVAVVWAENANPDPRLHNRLKNVSEKLDVPPLQEELRALVDWVANYTL
                     SARGMVLRMCLRMGEHLGPERVRMGVRLIGAPPARMTPARRRLIELLSDGLLHGKSEA
                     AKEAGVSAGVIDGLVDEGTLATEGMPRAPAPAAPDPSFSAPEFSRQQRTAVDAMRALA
                     ANGSFHVALLDGVTGSGKTEVYFEAVAETIRRGKQSLILMPEIALTGQFLDRFAQRFG
                     VRPLEWHSELTPRTRQRNWAAIAAGEAPVVVGARSALFLPYAGLGLIVVDEEHDQAYK
                     QDEGAHYHARDMAVVRAHIAKIPIVLASATPSVETEVNARKGRYQRAVLPSRFGGQHM
                     PQIEAIDLRREAPARGRFISPRLAEQIQIAVERREQALLFLNRRGYAPLTLCRACGHR
                     FACTICDAWLVDHRFRQRLVCHHCGFSMPRPDVCPHCAAEQSLVAVGPGVERLQEEVA
                     SLFPEARTMVLSSDLVTSIETMRSELNEIAEGRVDIIIGTQLVAKGHHFPRLNLVGVV
                     DADLGLSNGDPRAAERTFQLLNQVIGRAGRDRGRGIGYLQTHQPEHPVMKALVACDRE
                     AFYASEIEARERAFYPPFGRLASLIISAGDRSTAESFARRLAAIAPIDQRIQVLGPAE
                     APLAVIKGRYRFRLLVKSARGVDLSEYLRDWLAAAPKTKGNLKLEVDVDPQSFL"
     gene            complement(7236..7736)
                     /locus_tag="VN968_15255"
     CDS             complement(7236..7736)
                     /locus_tag="VN968_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXL30629.1"
                     /translation="MLEFKNLSLGLATQSRAAMTILAATLFVGGGITEASAKSRHHHR
                     HHHSHQSSSSAGGSWRDANAAVTSSSGSGHSFSGMASFYGNESGSKTASGQRFNQNAL
                     TAAHRSLPFGTKLRVSHGGRSVVVTINDRGPFVRGRVLDLSTGAARAIGLTSAGVGRV
                     TAEVVS"
     gene            8018..8317
                     /locus_tag="VN968_15260"
     CDS             8018..8317
                     /locus_tag="VN968_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30630.1"
                     /translation="MTRESSRSCKWLMRPMQVRLEQMSFPGKSSQSDVRAMAVGILPV
                     AEVSKMSRENRRQWTCDKASPVAAVMLHLRVRYVSKAAILTLRVPVVPIENRSPA"
     gene            8398..8958
                     /locus_tag="VN968_15265"
     CDS             8398..8958
                     /locus_tag="VN968_15265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HXL30631.1"
                     /translation="MAAEDPTVSGVSGRYATALFELARDEKSIDAVKTDLDGFDALLS
                     ESADLKRLVRSPVFSADAQLKALTAVLDKAGITGIAANFLKVLTRNRRLFAISDVSRA
                     FRALVAKFKGEATADVTVAERLSERNLDALKTALKSVTGKDVALNVKVDPSIIGGLVV
                     KLGSRMVDSSLRTKLNSIKHAMKEAG"
BASE COUNT         1650 a         2698 c         2923 g         1726 t
ORIGIN      
        1 gatcctgaac gcgccgcagt ccggcattct cggcatgcac aagattcagg atcgcccggt
       61 cgcgatcggc ggcaaggtcg agatacgccc gatgatgtat ctggcgctgt cctacgatca
      121 ccgcgtcatc gacggcaagg atgcggtaac gttcctggtg cgcgtcaagg agagcctgga
      181 ggatccggcg cggctggtgc tggacttgtg aaaagattcg gaatcttgat ttttgcactc
      241 gtcgcgatgc cggggcggct tttacagcag agcgcgtttg ttttgtgggg gcaaatttga
      301 cggacagggt tgcagtcatc accggcggaa gccgcggcat cggccgcgcc accgcgatct
      361 ccgccgcggc gcggggcttc aaggtctgcg tcggctatgc cagcaatgaa gcggctgcgc
      421 gccaggtggt ctccgccatc gaggcgaaga acggcaaggc gattgcgctg aaatgcgatg
      481 tcggcaacga gagcgatatt ttggcgctgt tcaaggccgc cgatgcgttt ggaacgctgg
      541 gcgcccttgt caacaatgcc ggcatcgtcg gcgcgacgtc gcgggtcgac gaaatgtccg
      601 ccgagcgcat tcaacgcatg atggcggtca acgtcaccgg cagcattctg tgcgcgcgcg
      661 aagcggtgag gcggatgtcg acccgccacg gtggcaaggg cggggtcatt gtcaacctgt
      721 cgtcggtcgc ctcaaagctc ggttcgccga atacctatgt cgactatgcg gcatccaagg
      781 gcgcgatcga ttccttcacc atcgggctgg gatacgaagt cgccggcgaa ggcattcgcg
      841 tcgcggcgat ccgccccggg ctgatcgata ccgaaattca cgcgagcggc ggcgagcccg
      901 accgcgccca ccgccttagt cacatggtgc cgatgaagcg cgtcggcacc gcggacgaaa
      961 tcgccaacgc catcgtctgg ctgatgtcgg acgatgcctc ctacgtgacc agcgcgattc
     1021 ttgatgtgtc gggcgggcgc taatacctct cacagtcttc agctacagga ctccaatcat
     1081 ggccacttac gatcttgttg tcatcggcac aggtccgggc ggatatgtct gcgcgatccg
     1141 cggggcgcag ctcggcatga aggtcgccgt ggtcgagaag gatgcagcgc ttggcggcac
     1201 ctgcctcaat gttggctgca tgccgtcaaa ggcgctgctc tatgcatcgg agatgttcga
     1261 ggaggccgcg cactcgtttg cgaaaatggg cgtcagcgtc tcagcgccaa agctcgatct
     1321 tccctcgatg atgaaattca agcagcaggg catcgacggc aacgtcaagg gcgtcgagtt
     1381 cctgatgaag aagaacaaga tcgacgtcat cagcggcaag ggaaggatac tcggtaccgg
     1441 caaggtcgag gtcaccggca atgacggcaa gacgcagacg gtcgagacca aaaacatcgt
     1501 catcgccacc ggctccgaca tagcgcctct caagggcatc gaaatcgacg agaagcgcat
     1561 cgtgtcgtcg accggcgcgc tttcgcttga cagggtgccc gaacggcttc tgatcgtcgg
     1621 cgccggcgtg atcgggcttg agctcggctc ggtgtggcac cggctcggtg cgcaggtcac
     1681 ggtggtggaa tttctcgatc gcatcctgcc gggcaatgac ggcgaagtgg caaaacaatt
     1741 tcagcgcatt ctcgagaagc agggctttgc cttcgaactg agtaccaagg tcaccgccgt
     1801 cgacacatcg ggcaaaacgc tgatcgccca ggtcgaaccg gccgcgggtg gcaagcctga
     1861 aaagctggag gccgacgtgg tgctggtctc gatcggccgc gttccctaca ccgagggtct
     1921 cggcctgaag gaagccggtg tcgtgctcga caatcgcggc cgcgtgcaga tcgacgcgca
     1981 tttcgccacc agcgtgaagg gcgtatatgc catcggtgac gtcgtggcgg gtccgatgct
     2041 ggcccataag gccgaggacg aaggcgtcgc ggttgcggaa atcctcgccg gccaggcggg
     2101 gcatgtgaat tacgacgtta tcccgagcgt ggtgtatacc acgccggaag tgtcggcggt
     2161 cggcaagact gaagaggagc tgaaacaggc ggggatagcc tataccgtcg gcaaattccc
     2221 cttcaccgcc aatgggcgct ccaaggtcaa ccagaccacc gagggattcg tgaaggttct
     2281 cgcagatgcg aaaacggatc gggtgcttgg cgtgcacatt gtcggccgcg aggccggcga
     2341 aatgatccac gaagcggccg tcctgatgga gtttggcggt tccgccgagg acctggcccg
     2401 cacctgccac gcccatccga cccgttcgga ggccatcaag gaagcggcgc ttgcagtcgg
     2461 caagcgctcg atccatatgt gagcgacgcc cccggggaag gacgggctgg gacaaccgtc
     2521 atgatgcgcc gccttcttca accggtctgg gttctgctcg cggtcatctt cctgatcgag
     2581 gcttggctgt gggatcacct cgagccggtc gtggcgtggt tcgtggcgct gattccgctg
     2641 cgcgccttca aggcctggct ggccgagcgg gtcgatacgc tgtcgccggc gatgacgctg
     2701 atcgtgttca tcgtgccggt ggttccgctg tttccgctga agctggtcgc tctttggttg
     2761 ctcgcccatg aatattggat ggccgctgtc ctcaccttcg tgttcgcaaa attcgtgggt
     2821 gtcggtgtcg cggctttcat cttcgacgtg acgcggccga aattgctcga gatggagtgg
     2881 ttcgagaaac tctacgagtt tgtcattgcc ttgcgggcga aggccgtggc actggtcgat
     2941 ccaatcaaat tgcgaatcaa agaggtattg agcggcggcg gcgatggctg gtcatcacgg
     3001 ttgctgcggc tgatccaacg ctttcgcaaa agcgtgcacg aagcgcgcta gagtctgttt
     3061 caaccagcct tcacatcaca tgcaccgcgt gcggcgccaa caggccgatc gcggtaaacg
     3121 ccagtccggc aagcgtcaac aagcccgata tccaggccag cgcgacaatg ccggtgataa
     3181 tggtcgccga cgccagcacg atgccgatct gaaatgcggc agaggcgagt tcgtagtgat
     3241 ggtatctggc cagcgcgagg tcgcgctcgt gttcggcatg cttggcgcgc tcggatagct
     3301 gctcggagcc ttcgccggtt tccggctcgg agcggtagcg tgccgcggtc ttctgccatt
     3361 cctcgatctg cttttgcagt gccgtcttgg cggcatcgtc ccccatcagg ccgaggctca
     3421 atctcgcctg ttccgacacc gcctggacca ccgtgcggcg gatcgtcttg gcctgaaaga
     3481 acgcccagag attggaggct tcgacattct tgctaatcgc ttcggtctgg gcgctcttgc
     3541 ccagtgtctc cgacatcgcc agaaacagcg cgatcacggc gatcagcaac gcgatctttt
     3601 tgtttccgct ggaggcgtgc tcggcgctct ccgcatgctc catgctttca tgtgcgctca
     3661 tgattcgctc ccgatcgaca acgccacgat tgaccgaaca atgccgcctg cgcaagaggc
     3721 aagcggatgt gacgggagca tgtcggggat catcgatctc aggcgcgggg gtgcgtcgtt
     3781 ttatagactt ccagcagccg ctcgctgtcg acgccggtat aaatctgggt ggtcgagagc
     3841 gacgcgtggc cgagcaattc ctggatcgcg cgcaggtcgc cgccgcggct gagaagatgg
     3901 gtcgcgaaag aatgccgcaa cgcgtgcggc gtggcgctgt cgggcaggcc cagcgcgccg
     3961 cgcagccgtt ccatggtgag ttgaatgata cgggccttaa gcgggccgcc tcgggcgccg
     4021 acaaagatcg ggccttgggg cggcagcgga tgcggacaga tcgccacata atcctgaacc
     4081 agcgccagca cattttgcag caccggcacc atgcgggcct tgttgccctt gccgttgacg
     4141 acaagcacgt cgccggcgcc gggcaaaggc acgtcgcggc gcttcaggcc aagcgcttcg
     4201 gaaatgcgca ggcccgatcc gtacagcagc gccattaccg cggcgtcgcg cgcaaggatc
     4261 cagggatcgc gggtctctcc ggcgcgctca tcggcgtccg ccaaacgttt ggccgccgcc
     4321 atatggatcg gtttcggcag gctcttcccg acctttggcg cgcggatcgc cgacagcgcg
     4381 ccgaccttgc ctcggccttc ctgctccagg aagcgagcga acgagcgcaa accggcaagc
     4441 gcccgcatca gcgaacgtcc accgatatcg tgtccgcgcc gcattgccat gaaggccctg
     4501 acgtcgctcg cctcgagcgc cgcaagacgc gaaagcgtca cccgtgaacc ccagtgctca
     4561 ccgagaaagc cgagacattg gcggacgtcg cgggcgtatg cttccagcgt ctttggcgac
     4621 agccgtcgtt cgctgcgcag atgcgacagc cagcgcgtca tctcccgcgc gataccatcg
     4681 tcggcgcagt caagttcgat ctgctgagtg gcgggagccg attttgtcat aacttgcctt
     4741 ggggccgagg atgattcccg gtgattatat cgcacctctt tcgtttaccg ttcgctaact
     4801 tgcgaaagcg gcagcgtcgg cactggccct gaccgccgca cggtcctaag ttaatgcggc
     4861 ccttccggct tcgagcctga ttctccaaag ctgattcttc aatggaccat tccatccgtg
     4921 ccagcacctc atccgcatcg tcggcgcgtg tcgtcgacgt gctggtgccg gttgcgctca
     4981 atcagaccta ttcctaccgg gtgccgcgcg gcatggatgt ggcgccgggc gatgtggtgt
     5041 gcgtgccgct ggggccgcgc gaggcggtcg cggtggtgtg ggcggaaaac gccaatcccg
     5101 atccacgcct gcacaatcgc ctcaagaacg tcagcgagaa gctcgacgtg ccgccgttgc
     5161 aagaggagct gcgcgctctc gtcgattggg tcgccaacta cacgctgtcg gcgcgcggca
     5221 tggttttaag gatgtgcttg cggatgggcg aacatctcgg ccccgagcgg gtgcggatgg
     5281 gcgtgcgtct gattggcgcg ccgcctgcgc gcatgacgcc ggcgcggcgc cggctgatcg
     5341 agctgctgtc ggatgggttg ttgcacggca aatccgaagc tgcaaaggaa gccggcgtca
     5401 gcgcgggcgt gatcgatggt ctggtcgatg aaggcacgct ggcgacggaa ggaatgccgc
     5461 gcgcgccggc gccggctgcg cccgatccgt cgttctcggc gcccgagttt tcccgccagc
     5521 agcgcacggc cgtggatgcc atgcgcgcgc tggctgcaaa cggcagcttt cacgtcgcac
     5581 tgctcgatgg cgtcaccggg tcgggcaaga ccgaagttta tttcgaggcg gttgcggaaa
     5641 ccatccggcg cggcaagcag tcgctgatcc tgatgccgga gatcgcgctg accggccagt
     5701 tcctcgaccg cttcgcgcag cgtttcggcg ttcgcccgct ggaatggcat tccgaactga
     5761 ccccgcgcac aaggcaacgc aattgggcgg cgattgccgc cggcgaagcc cccgtcgtgg
     5821 tcggcgcacg ctccgcgctg tttctgccct atgccggtct cgggctgatc gtcgtcgacg
     5881 aagagcacga ccaggcctac aagcaggacg aaggcgcgca ttaccatgcc cgcgacatgg
     5941 cggtggtgcg cgcccatatc gcgaaaattc cgatcgtgct ggcgtcggcg acgccctcgg
     6001 tcgaaaccga ggtcaatgcc cgcaagggcc gctatcagcg cgcggtgctg ccgtcgaggt
     6061 tcggcggcca gcacatgccg cagatcgagg cgatcgatct gcggcgcgaa gcgccggccc
     6121 gcggccggtt catatcgccg cggctcgccg agcaaattca gatcgcagtg gagcggcgcg
     6181 agcaggcgct tttgtttctc aaccgtcgcg gctatgcgcc gctgacactg tgccgggcct
     6241 gcgggcatcg ctttgcctgc accatctgcg acgcctggct ggtcgatcat cgctttcgcc
     6301 agcgtctggt ctgccatcac tgcggcttct cgatgccgcg cccggatgtc tgcccgcatt
     6361 gcgcggcgga gcaatcgctg gtggcggtcg gccccggcgt cgagcgcttg caggaggagg
     6421 tagccagcct gttcccggaa gcgcgcacca tggtgctgtc gagcgacctg gtcacctcga
     6481 tcgagaccat gcgcagcgag ttgaacgaga tcgcggaagg gcgggtcgac atcatcatcg
     6541 gcacgcaatt ggtggcgaag ggccatcatt ttccgcggct caatctggtc ggcgtggtcg
     6601 atgccgatct cggcctaagc aatggcgatc cgcgcgccgc cgagcgcacg tttcagctgc
     6661 tcaaccaggt gatcgggcgc gccggtcgtg accggggccg cgggatcggc tatctgcaga
     6721 cccatcagcc cgaacatccc gtgatgaagg cgctggtggc atgcgaccgc gaggcgtttt
     6781 acgccagcga gatcgaggcc cgcgaacgcg ccttctatcc accgttcggc cggctcgcca
     6841 gcctgatcat ctccgcgggc gatcggtcta ccgcggaaag ctttgcgcgc aggttggccg
     6901 cgatcgcgcc gatcgaccag cggatccagg tgttgggacc cgccgaagcc ccgcttgcgg
     6961 tgatcaaggg ccgctatcgg tttcggctgc tggtgaagtc ggcgcgcggt gtcgatctgt
     7021 cggagtattt gcgggactgg cttgccgccg ccccgaagac caagggcaat ctcaagctcg
     7081 aggtcgatgt cgatccacag agctttttgt gacgaattga aaaaggcctg ccgtcatttc
     7141 tgacggcagg cctgtattgg cgtgcagcaa gttctgcggc cgcaatggac gcaacgctta
     7201 ccgaagttga atggatggaa gaggcgtaaa acgccttaag agacgacctc tgcagtgacg
     7261 cggccaacgc cggcactggt caggccgatc gcgcgggcgg cgcccgtgga caggtcgagc
     7321 acccggccgc ggacgaacgg gccgcggtcg ttgatggtca cgacgacgct gcggccgcca
     7381 tggctcactc tgagtttggt gccgaacggc agcgaacggt gggccgccgt cagggcgttt
     7441 tgattgaaac gctggcccga ggcggtcttg ctgccggatt cgttgccgta aaaggaagcc
     7501 atccccgaga agctgtgtcc ggagcccgat gatgaggtca ccgcggcatt ggcatcccgc
     7561 caagaaccac cggcagaact gcttgattgg tgagaatggt gatggcggtg atgatgcctg
     7621 gatttggccg aggcttcggt gatgcccccg ccgacgaaaa gagttgcggc aaggatagtc
     7681 attgccgcgc gcgactgggt tgcaagtccc agtgacagat ttttgaattc cagcatatag
     7741 taagtccctc gagatagtat tgccacatat gtggcaagtg gaacccccgt cccgttttga
     7801 gtgatcgccg tttggtgggc taatgaggcg taagctgggc agtaattccg ttttgttcca
     7861 cactgaaaca tcttgttact agcagggtta atccacaccg cgtttcaaaa gtctgtaaaa
     7921 tatcaatttc tatactttgt aattactcat atgtactgac aaggtaagtt gtgctcactg
     7981 atgacagcgt cgtgagcaag taattgcggg tgatggcatg acccgggaat ccagtcgttc
     8041 ctgcaaatgg ctcatgcggc ccatgcaggt gcgcctggaa caaatgtcct tcccggggaa
     8101 aagctcgcag agcgatgttc gcgcgatggc ggtcggcatc ttgccggtgg cagaggtgtc
     8161 gaagatgtcg cgtgaaaatc gccgccagtg gacgtgcgac aaggcatcgc cagtcgccgc
     8221 cgtcatgttg cacctgcgcg ttcgctatgt tagcaaagcc gcgattttaa cgcttcgggt
     8281 accggtcgta ccgatcgaaa atcgaagtcc cgcttgaatt ccaagggctt ggatcgctag
     8341 gcgccgcatc gtggcgacgg tttttccctt gcgagtttga cagcaaagag cgcgcttgtg
     8401 gctgctgaag atccgaccgt ttcgggagtg tccggccgtt atgcaacggc cctgtttgaa
     8461 ctggcgcgcg atgaaaaatc catcgatgcg gtaaagaccg atctcgatgg attcgacgcc
     8521 ctgctcagcg agagcgccga tctcaaacgg ctggtccgca gcccggtgtt ttcggcggac
     8581 gcgcagttaa aggcgctcac cgcggttctc gacaaggccg gtataacagg catcgccgca
     8641 aatttcctga aagtcctcac ccgcaaccga cggctgttcg caataagcga cgtgagccgt
     8701 gccttccgcg cgctggtagc caagttcaag ggcgaggcga cagccgacgt cacggttgcc
     8761 gaacggctca gcgagaggaa tctcgatgcc ctgaagaccg cgctgaaatc ggtgacgggc
     8821 aaggacgtcg cgctcaacgt gaaggtcgat ccctcaatca tcggcggcct tgtggtcaag
     8881 ctcggcagcc gcatggtgga tagttcgctt cgcaccaaac tcaattcgat caagcacgcg
     8941 atgaaagagg caggctgatg gacatccgcg ccgcggaaat ttccgcgatc ctcaagg
//
LOCUS       DATNOI010000385         4920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1979897,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000385 DATNOI010000000
VERSION     DATNOI010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1979897"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="VN968_15270"
     CDS             <1..1239
                     /locus_tag="VN968_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HXL30632.1"
                     /translation="KGDPADWAAAAKGILATEKPDAIVIMLGLNDRVSMREPVVEKSD
                     AKSTDKKSDKKDARTKPDAKPGDAKPGAKPDGAADAAARPEDKADTELSPDDAADNGD
                     APPAIAPEKSTRSPNGVYEFREERWVELYVKKIEEMIGVMKSKGVPVLWVGLPAVRGT
                     KATADMLFLDSLYRDAAGKAGITYVDVWDGFVDEAGRFLQRGPDFEGQPRKLRADDGV
                     FFTRPGARKLAHYVEREITRLLAARSAPIALPTEPATPDANALPGQPAPRPLAGPILP
                     LVASSVGTDQLLGGPGSRPAAVDALAARTLVKGEPLAPPAGRADDFVWPRREVGREQA
                     KGETPVASTSPDGTVAAPGGPPKPAKRLRPVQSGTPALREFFGFGNAPRQPPPLRAPG
                     PPRPPGTVGRSASVPDFFMR"
     gene            complement(1363..1737)
                     /locus_tag="VN968_15275"
     CDS             complement(1363..1737)
                     /locus_tag="VN968_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HXL30633.1"
                     /translation="MDKDRIAGSAKDIAGKVEGTVGDMAGDAQAQAAGRVREAAGTVQ
                     NLYGQAKDAARDAGDAAASYAKDAYENSGETLREGSEVVAKKVQDNPLGALLIAGGIG
                     FALAMLMTRPPRRPPQRWRYYG"
     gene            complement(1868..2602)
                     /locus_tag="VN968_15280"
     CDS             complement(1868..2602)
                     /locus_tag="VN968_15280"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL30634.1"
                     /translation="MNELDFSAKQVLVVGGSSGIGNGIAQAFRAKGARVHVCGTRAGA
                     GDYSVSEGSDLAGLNYAQLDVNDTKAIEEFKPTFDRLDVLVLAQGAVIYRRGEFEMSG
                     FRHVLEVNLISLMACATRFYQMLSAANGSLIIISSTAAYHSTKGNPAYNASKTGAVGL
                     TRTLGEAWAEDGIRVNGIAPGLVDTKMTKVTTENPKRLEGAIGRIPLKRLGTPADMAG
                     AALFLASPLAAYIVGQTLVVDGGLIL"
     gene            2699..3478
                     /locus_tag="VN968_15285"
     CDS             2699..3478
                     /locus_tag="VN968_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL30635.1"
                     /translation="MSDFKQFNRSVAGLTVLVTGAAGGMGRATARVFAAEGAKVAVTD
                     FSAEGAQAVAAEIVANGEIAKAWRLDVANRDDVNAVVNDIAAHFGGLDIVVNNAGISV
                     RVAIDDDGYEEAWAKGLAVMLTAHPRVIRAALPYLRKSKCPRIVNIASTEALGATALH
                     SPYSAAKAGVVGLTRSLAVELGRDGITVNCVCPGPISTGMTARISDEHKAIYARRRTA
                     LGRYGHPEEVAHMTLSLCLPAASFLTGAIIPVDGGLMARNA"
     gene            3593..4279
                     /locus_tag="VN968_15290"
     CDS             3593..4279
                     /locus_tag="VN968_15290"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXL30636.1"
                     /translation="MSALIFDFDGVIADSEALANTVLAEFLTDLGHATNLEDSLEHYS
                     GRRWDDAIAAIESAVGKPLPGSFSDDLKLAILDRFRTDLTEVSGASKFIQTFSDVPRC
                     IASSSSIDRLQLCLSILNLTDKFGSNVFSADMVSRGKPHPDIFLLAADRLGVNPKSCL
                     VIEDSTSGIKAAVAAGMTAVGLCAASHIRGGHQLKLREAGAVHLAKSWDEVAKIAAQF
                     LDGASRHLPA"
     gene            4371..>4920
                     /locus_tag="VN968_15295"
     CDS             4371..>4920
                     /locus_tag="VN968_15295"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HXL30637.1"
                     /translation="MTVSIRQLHPHFFGEVSGVDLRQPLTPQEAADIEAGMDKCAVLL
                     FRNQDITDEQQMAFALNFGERENPRGGNIVKPQDSRLTTGLNDVSNLDKDGKPLPRDS
                     RVHLFNLGNCLWHSDSSFRPIPAKFSLLSARVVNPKGGNTEFADMRAAYDALDDETKA
                     EIEDMICEHSLMYSRGSLGFLDY"
BASE COUNT          911 a         1592 c         1508 g          909 t
ORIGIN      
        1 aagggcgatc cggccgactg ggccgccgcc gcgaaaggca tcctcgccac cgagaagccg
       61 gacgccatcg tcatcatgct cgggctcaac gaccgcgtct cgatgcgcga gcctgtggtg
      121 gagaaatccg acgctaaatc aaccgacaaa aagagcgaca agaaggacgc tcgcaccaaa
      181 ccggatgcca aacctgggga cgcgaaaccc ggggcgaagc cggatggcgc tgcggacgcc
      241 gccgccaggc ctgaggacaa agccgatacc gaattgtcgc ccgacgacgc cgccgacaat
      301 ggcgacgcgc cgccggccat cgcgccggag aagagcaccc gctcgccgaa cggcgtctat
      361 gaatttcgcg aggaacgctg ggtcgaactc tacgtcaaga aaatcgaaga gatgatcggc
      421 gtgatgaaat ccaagggcgt gccggtgctc tgggtcggcc tccccgcggt acgcggcacc
      481 aaggcgacgg cggacatgct gttcctggat tcgctgtatc gcgacgctgc cggcaaggcc
      541 ggcatcacct atgtcgatgt ctgggacggg tttgtcgacg aggccggccg cttcctgcag
      601 agaggccccg actttgaagg ccagcctcgc aaactgcgtg ccgatgacgg cgtcttcttc
      661 accaggcccg gcgcacgcaa gctcgcgcac tatgtcgagc gcgagatcac ccggctgttg
      721 gcggcgcgct ccgcgccgat cgcgctgccg accgaacccg cgacgcccga cgccaacgcg
      781 ctgcccggtc agccggcgcc gcgtccgctg gcgggaccga tcctgccgct ggtagcctcc
      841 tctgtcggca ccgaccaatt gctcggcggc cccggctccc gcccggccgc ggtcgacgcg
      901 ctggcggcac gaacgctggt caagggcgaa ccgctggcgc ctcctgccgg ccgcgccgat
      961 gatttcgtct ggccgcgccg cgaggtcggg cgcgaacagg ccaagggcga aacgccggtg
     1021 gcgtccacct cgccggatgg aacggttgcg gcacctggcg ggcccccgaa gccggcgaaa
     1081 aggcttcgcc ctgtccaatc gggtacgccg gcgttacggg agtttttcgg ctttggaaac
     1141 gcaccacgcc aaccgccgcc gctgcgtgcc cccggtccgc cgcgcccgcc gggcacggtc
     1201 ggacggtcgg cctcggtacc ggactttttt atgcggtgac ttgattgatc ctcatggtga
     1261 ggagcgcgtc ttcgcgcgtc tcgaaccatg tggccgaaca tttcccatcc ttcgagacgc
     1321 ggccaagagg ccgctcctca ggatgaggag tccgtgattg cttcagccgt aataccgcca
     1381 gcgctgcggt gggcgacgcg gcggacgcgt catcagcatc gccagcgcaa agccgatgcc
     1441 acctgcgatc aacagcgcac cgagcggatt atcctgcacc tttttcgcca cgacctccga
     1501 accctcgcgt aaagtctcgc cgctgttctc ataggcgtcc ttggcgtagc tggcggcggc
     1561 gtcgccggcg tcacgcgctg catccttggc ctggccgtac agattctgca ccgtgccggc
     1621 ggcctcgcgg acacggcccg cagcctgcgc ttgagcgtcg cctgccatat cgccgacagt
     1681 gccttcgact ttaccggcaa tatcctttgc cgagccggca attcgatcct tgtccatcgc
     1741 gctcactcct tgttacgcgc cttgcgaaat acgcgccttg agatgattct cggtgagcta
     1801 accgatcagg cccgttccgg ttccggtaat gcgtcgatac gccttggcca caggcgaatg
     1861 cgatccttca gagaatcaaa ccgccgtcga ccaccagggt ttgaccgacg atataggccg
     1921 caagcggcga cgccaggaac agcgcggcgc ccgccatgtc ggccggtgtt cccagccgtt
     1981 tcagcggtat ccgtccgatc gcgccttcga gccgcttcgg attttccgtc gtcaccttgg
     2041 tcatcttggt atcgacgagg ccgggcgcga tgccgttgac gcggatgcca tcctcggccc
     2101 acgcctcgcc gagcgtccgg gtcaatccga cggcgccggt cttcgaggcg ttgtaggcgg
     2161 gattgccctt ggtcgaatga taggcggcgg ttgaactgat aatgatgagc gaaccgttgg
     2221 ccgcactgag catctgataa aatcgggtcg cgcacgccat caggctgatc agattgactt
     2281 ccagcacatg acgaaatccc gacatttcga attcgccgcg ccggtagatc accgccccct
     2341 gcgccagcac cagcacatcg agccggtcga acgttggctt aaattcttcg atcgccttgg
     2401 tatcgttgac gtcaagctga gcgtaattca gacccgcaag gtcggaaccc tcgctcacgg
     2461 aatagtcgcc cgcgccggcg cgggtgccgc agacatgcac gcgcgcgccc ttggccctga
     2521 acgcctgcgc tatgccgttg ccgatgccgc tggagccgcc aacgacaaga acctgctttg
     2581 cgctgaaatc cagttcgttc atctccccgc gatccttttt tgcattgttt gacctctctg
     2641 gagatcggtg ccagccttgt caatcctgca gtgcacgaca ggcttgagcg agtattccat
     2701 gtccgatttc aaacaattca accgctcggt cgcgggattg accgttctcg tcaccggcgc
     2761 ggccggtggc atgggccgcg ccaccgcgcg ggtgttcgcg gccgaaggcg ccaaggtggc
     2821 ggtcaccgat tttagtgcgg aaggcgcgca agcggtcgct gccgagatcg tcgccaacgg
     2881 cgagattgca aaagcgtgga ggctcgacgt ggccaatcgc gatgacgtca acgctgtcgt
     2941 gaacgatatc gccgcgcatt tcggcgggct cgacattgtc gtcaataacg ccggcatttc
     3001 ggtgcgagtg gcgatcgacg atgatggcta tgaggaagcg tgggcaaagg ggcttgccgt
     3061 gatgctgacg gcgcatccgc gggtgatccg cgccgcgctg ccctatttgc gcaaatcaaa
     3121 atgcccacgg atcgtcaaca tcgcctccac cgaggcgctc ggcgccaccg ctttgcacag
     3181 cccctattcg gcggcgaagg cgggcgtggt tggattgacg cgttcgctcg cggtcgaact
     3241 cggccgcgac ggcatcaccg tgaactgcgt ctgccccggc ccgatctcca ctggcatgac
     3301 ggcacgcatc agcgacgaac acaaggcgat ctatgcgcga cgccgaaccg cgctcggccg
     3361 ctacggccac cccgaggagg tcgcccacat gacgctgagc ctctgcctgc ccgcggcttc
     3421 attcctgacc ggcgcgatca tcccggtgga cggcggcctg atggcgcgga atgcgtgaga
     3481 tggacgctag gctggttgac acaacgtgaa acagaatttg ttcggcgaca accgctccag
     3541 ccgatggatg gatgcactat aaccgggtcc acgctcgcgc tggggcttca aattgagtgc
     3601 attgattttc gattttgacg gcgtgattgc cgacagcgag gcgctggcca acaccgtgct
     3661 ggcggaattc ctcactgatc ttggtcacgc aacaaacctc gaggactcac ttgaacacta
     3721 ctccgggcga cgatgggacg atgccatcgc ggcgatcgag tccgcggtag gcaagccgct
     3781 gccggggagt ttttcggacg atctcaagct tgcgattctt gatcgatttc gtaccgacct
     3841 cacggaggtc agcggagcca gcaaattcat tcagacgttc tccgacgtac cccgatgcat
     3901 cgcgtcctcc agttcgatcg atcgcttgca gctctgtctt tcgatcctca atctcacaga
     3961 caaattcggc agcaacgtat tcagcgcgga tatggtgtcg cgcgggaagc cgcacccaga
     4021 catatttttg ctcgcggccg acagactagg ggtgaacccc aaatcatgcc tcgtcatcga
     4081 agacagcacg agcggcatca aggcggcggt ggctgcgggc atgacggctg tgggcctgtg
     4141 cgcagcctcg catattcgcg ggggccatca actcaagctc cgtgaagcag gtgccgtgca
     4201 cctggccaaa tcctgggacg aagtggcaaa gatagcggcg cagtttcttg acggagcttc
     4261 gcgccatctg ccggcatgac ctgcagagcc ggttgacatc gcaccatcag ggagtagttt
     4321 tttacgcaat gagagcggaa caaccgcatc cctcgatcag gagaaacgtc atgactgtct
     4381 cgatccggca gcttcacccg cattttttcg gcgaggtttc cggcgtcgat ttgcgacagc
     4441 cgctgacgcc gcaggaggcc gctgacatcg aggccggcat ggacaaatgc gccgtactgc
     4501 tgttccgcaa tcaggacatt accgacgagc agcagatggc gttcgcgctg aatttcggcg
     4561 agcgcgagaa tccgcgcggc ggcaatatcg tcaagccgca ggattcccgg cttactaccg
     4621 gccttaacga tgtttccaac ctcgacaagg acggcaagcc gctgccgcgc gatagccgtg
     4681 ttcatctgtt caatctcggc aactgcctgt ggcattccga cagttcgttc cgtcccattc
     4741 cggcaaaatt ttccctgctg tcggcgcggg tggtgaaccc caagggcggc aataccgaat
     4801 ttgccgacat gcgtgcggcc tatgacgcgc tcgacgacga gaccaaggcc gagatcgaag
     4861 acatgatctg cgagcattcg ctgatgtatt cgcgcggctc gctgggtttt ctcgattaca
//
LOCUS       DATNOI010000386         7241 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1121130,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000386 DATNOI010000000
VERSION     DATNOI010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7241
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121130"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VN968_15300"
     CDS             complement(<1..910)
                     /locus_tag="VN968_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30638.1"
                     /translation="MRFRDIAGAAYRSLTARRDGPSLYDVCDPVLLKPSGGDPHLSKF
                     YRTALGNPALRPLLRRTGLPELRDPRRIQSLSQALIRARDDETPDWAAIGQPVAALLD
                     TIDLEHPKPGPAPAPARAPDLAEIESVIRTCGGHLLRSFGQNGFIPTYAAFNLMGDPD
                     VGGREVLMALNGLDARGYKNSTLLFNLARVFIAHSPARALIRPAWTGIAEPMWQPMQI
                     RHRSAYYDAFFTEALLSFVDTGLASRSETAASRRAIAEMVDFCLTTSREDVRGQDGRI
                     VSVVTALAPLPHPRFSRFFSQIKQDLG"
     gene            1206..1586
                     /locus_tag="VN968_15305"
     CDS             1206..1586
                     /locus_tag="VN968_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30639.1"
                     /translation="MQILSMRSLVTGPNKPANPQPVAADASSITYNSLKCGVKTLQPK
                     CDINHTARSKCGGKMHGTGHAAVIEKCRRALPNGRLRVNGLFAAVPQIDYGVPQIERN
                     WAAQDRVPPTNLDGFGATNWRARL"
     gene            1608..2483
                     /locus_tag="VN968_15310"
     CDS             1608..2483
                     /locus_tag="VN968_15310"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXL30640.1"
                     /translation="MNVTQLCLSRRSITMAAAFLGMVSLAIGAHAALNMRALDAAKAV
                     EAEQTGALKGASRIALVIGNGNYPDASAPLAQPINDARALTAALRRNGFDVDVVEDAG
                     KDDMNRAIGRLKSKIRPDSVVMLFFGGYGVQVGRESYMIPVDAVIWKEADVRRDGFSI
                     EQVLDVMKEQGARAKLVVVDASRRNPYERRFRKFSHGLAPIAAPDNALILTAATPGKV
                     ADDSAGPYSVLMTELLNNLNANLDTKTAAGVETVFNKTRVAISRASDGEQVPSVSSSL
                     SEDVRLEAANANAGG"
     gene            complement(2601..3632)
                     /locus_tag="VN968_15315"
     CDS             complement(2601..3632)
                     /locus_tag="VN968_15315"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL30641.1"
                     /translation="MKIAFGRASAGVVTAIALLSGISPTHAQKADPVAVQEHIATATA
                     AAKSDLLGPLGLCKTATPAPGPSFMDNYRAMVKEPPLAPMQVMDELYFLGSRWTTAWA
                     IKTAGGLIIVDAMDNADEAEHYIEGGLRKLGLDPAEIRYVVISHAHGDHYGGAQYLKQ
                     KFNPRIVMSAIDWNALEETQRDPLFGTKRNPLFGEPPKRDMAVADGDTIQLAGTALKF
                     HVIPGHTLGTLATVFTVHDRGEPHRAVAWGGTAYNFGPLPDRLQLYSDTTIKYRAILK
                     QENADIFLSNHVSFDDAVAKMAALKDRAPGQPNPFVVGRDAVQRFLTVLGECALATKA
                     SIGEDTPGR"
     gene            complement(3783..4823)
                     /gene="gtdA"
                     /locus_tag="VN968_15320"
     CDS             complement(3783..4823)
                     /gene="gtdA"
                     /locus_tag="VN968_15320"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HXL30642.1"
                     /translation="MEAVQKTPEREAFYKKIDGENLSALWNVMGDLITPEPKSACRPH
                     LWKFDAIRDYMTEAGRLITAKEAERRVLVLENPGLRGQSKITTSLFAGVQMVVPGDVA
                     PAHRHSQSALRFVLEGKGAYTAVDGERTAMEPGDFVITPSMTWHDHSNETSEPMFWLD
                     GLDIPMVQFFDASFAEGSNEDQQKITRPAGDSFARYGHNLLPVDGKRVSKTSPIFNYP
                     YSYTREALEQAKVRGEWDACHGLKLKFSNPETGDFAMPTIGTFIQLLPKGFKTARYRA
                     TDATVFAAIEGKGRSRIGEQTFEWGPRDLFVAPSWQWVTHEADEDSVLFSFSDRPVQQ
                     KLDLFREDRGNA"
     gene            4942..5574
                     /gene="maiA"
                     /locus_tag="VN968_15325"
     CDS             4942..5574
                     /gene="maiA"
                     /locus_tag="VN968_15325"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HXL30643.1"
                     /translation="MKLHGYFRSSASYRVRIALNLKGLSADHLPHHLRRGEQRDPAYV
                     AINPQGLVPTLQDNAGAIITQSLAIIEWLEETHPEPPLLPTDPLRRAKVRAFALVLAC
                     DTHPVQNLKVLARLRELGLPEDQVTGWAAWANREGLSACETLIANEPGPFCFGATPTM
                     ADLCLVPQLANARRFGVDLNAFPRLLKAETAAKNLKAFADAAPDKQPDAE"
     gene            5564..6073
                     /locus_tag="VN968_15330"
     CDS             5564..6073
                     /locus_tag="VN968_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXL30644.1"
                     /translation="MPSKTKPITMDAVYTAPGYLFRRMQQIAVAIFVEECKAYDLTPV
                     QYASLVAIRTHPGIDATRLSAVIAFDRSTLGSVIERLEAKDYIERKPAREDKRVKLLH
                     LTRQGVALLRDIMPSVDRAQARMLQPLKPADRKTLMALLTQLVDLNNEASRVPLRAED
                     ALEHLGKAN"
     gene            6073..>7241
                     /locus_tag="VN968_15335"
     CDS             6073..>7241
                     /locus_tag="VN968_15335"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HXL30645.1"
                     /translation="MGSDARPVLIAGGGIGGLAAALGLAQKGIPSILLEKASALGEIG
                     AGIQLGPNAFHAFDYLGVGEAARSMAVYIDQLRLMDALTADEITHVDLRDAFRSRFGN
                     PYAVVHRGDLHGVFLRACEAHDLIELRVSSEVLGYDQDGSSVTARLAAGGTVTGSLLI
                     GADGLWSNIRKKVTADGPPRVSGHTTYRSVIPTEEMPEDLRWNAATLWAGPKCHIVHY
                     PLSGWKVFNLVVTCHNDAAEPVAGKPVSDAEVMQGFAHVHARAQDIIRHGKNWRLWVL
                     CDREPTERWVDGRVALLGDAAHPMLQYFAQGACMALEDAVCLSHMLGACPDDHTRALE
                     QYRSQRFPRTARVQLLSRAIGEHIYHPAGEHARIRNAIMSAKSSEDYYRDLAWLYG"
BASE COUNT         1345 a         2270 c         2304 g         1322 t
ORIGIN      
        1 aaccgagatc ctgcttgatc tgggagaaga accgggaaaa gcgcggatgc ggcagcggcg
       61 ccagcgcggt gacgacgctg acgatacggc cgtcctgccc gcgcacatcc tcccggctgg
      121 tggtgagaca gaaatcgacc atctcggcga tggcgcggcg cgacgccgcg gtctcgctcc
      181 gtgaggcgag cccggtgtcg acaaagctca agagcgcctc ggtgaagaac gcgtcgtaat
      241 aggcggagcg atggcggatc tgcatcggct gccacatcgg ctcggcgatg ccggtccagg
      301 ccggcctgat gagggcgcgg gcgggcgagt gtgcaatgaa gacccgcgcc agattgaaca
      361 gcagcgtcga attcttgtag ccgcgcgcat cgagaccatt cagcgccatc agcacttcgc
      421 ggccgccgac atccgggtcg cccatcaggt tgaaggcagc ataggtcgga atgaaaccgt
      481 tctggccgaa cgatcgcaac agatgcccgc cgcaggtgcg gatgacgctt tcgatctcgg
      541 ccagatcagg cgcgcgcgcc ggcgccggcg cgggcccggg tttggggtgc tcaagatcta
      601 tcgtgtccaa aagcgccgcg accggctgcc cgatcgccgc ccagtccggc gtctcatcgt
      661 cgcgcgcccg gatcagggct tggctgagcg actgaatacg ccgtgggtcg cgcagctcgg
      721 gcagcccggt acggcgcagc agcggccgca gcgccggatt gcccagcgcg gtgcggtaga
      781 acttgctcaa atgcgggtcg ccgcccgagg gcttgagcag cacggggtcg cagacgtcgt
      841 acagcgacgg gccgtccctg cgggccgtca gcgaacggta ggcggccccg gcgatgtctc
      901 gaaatctcat ggcgtctctt cccgtgcgat cttgtcgcag atccgtctac ccggccaaat
      961 ccgtatcaag ggcttgccgc ccgccggttc ggaaggcgtg caagtccttg aaacgcacgc
     1021 caaccggaat aaaatacctc tgactttttt aacagaaggg tcgaataact tcagcgtcac
     1081 gctggtcgcg cacctacggt agcccggtac ggcgcgacag agggtcccag gcatcatagg
     1141 gcaacgcgcc gtccttgcgg gtggtcaggg ctcggcaggc tgccggggaa tgcgatgaag
     1201 attcattgca gattctttcc atgcgctccc ttgtcacagg tcccaataag cccgccaatc
     1261 cgcaacccgt ggctgccgat gcgtcttcga taacctataa ttctttgaaa tgcggcgtga
     1321 aaacgttaca accgaaatgt gacatcaatc acacagcaag gtcaaaatgt ggcggcaaaa
     1381 tgcatggaac cggccatgcc gccgtgatcg aaaagtgccg tcgagcgttg ccgaatgggc
     1441 gtttgcgagt taacggtttg tttgctgcag tgccacaaat tgattatgga gtgccgcaaa
     1501 ttgagcgcaa ttgggcggca caggatcggg tacccccaac aaacctagat ggctttggcg
     1561 cgacgaactg gcgcgcgcgc ctttgacact gaccagggat gaaacggatg aatgtaaccc
     1621 agctttgcct ctcccgccgc tcgatcacca tggccgccgc cttcctgggc atggtgtcgc
     1681 tggcgatcgg cgcccatgct gcgctgaaca tgcgggcgct ggatgcggcc aaggcggtcg
     1741 aagcggagca gaccggcgcc ctgaaggggg cgtcgcggat cgccctggtg atcggcaacg
     1801 gcaactatcc cgacgccagc gcgccgttgg cgcaaccgat caacgatgcg cgggcgctca
     1861 cggcggcgct gcggcgcaac ggctttgacg tcgacgtcgt ggaggacgcc ggcaaggacg
     1921 acatgaaccg ggcgatcggc cggctgaaat cgaagatcag gcccgattcc gtggtcatgc
     1981 tgttttttgg cggctacggc gttcaggtcg gccgcgaaag ctacatgatc ccggtcgacg
     2041 cggtgatctg gaaggaagcc gacgtccgcc gcgacggctt cagcattgag caggtcctgg
     2101 atgtgatgaa ggagcagggt gcccgggcca agctcgtcgt cgtcgacgcc tccaggcgta
     2161 atccgtacga gcgccggttc cgcaaattct cccatggcct ggcgccgatc gctgcgccgg
     2221 acaatgcgct gatcctgacg gcggccacgc cgggcaaggt cgccgacgat tccgcgggac
     2281 cctatagcgt gctgatgacc gagctgctca ataacctcaa tgccaacctt gacaccaaga
     2341 ccgccgcggg tgtcgaaacc gtcttcaaca agacccgcgt tgcgatttcg cgcgcgtccg
     2401 acggcgagca ggtcccgtcg gtgtcgtcgt cgctgtcgga agacgttcgc cttgaagcgg
     2461 cgaacgcaaa cgccggcggc taaagcatga tccggaaaag tgtgtaccgg ttttccgaaa
     2521 agatcatgct caaacaaaaa gattgagcgg gatgacgatt cgaagaaaag tcatcccgcg
     2581 ctagctggcg tttccagccg ctatctgccc ggcgtgtcct cgccgatgct ggcttttgtc
     2641 gccagcgcgc attcgccgag aacggtcagg aagcgctgca ccgcatcccg cccgaccacg
     2701 aacggattgg gctgacccgg cgcgcgatct ttcagcgccg ccatttttgc cacggcgtcg
     2761 tcgaagctga catggttcga caggaagatg tcggcgtttt cctgtttgag gatcgcgcgg
     2821 tattttatcg tggtgtcgga atagagctgc agccgatcgg gcagcggccc gaaattgtag
     2881 gccgtgccgc cccatgccac ggcgcggtgc ggctcgccgc ggtcatgcac cgtgaacacg
     2941 gtcgccagcg tgccgagcgt gtggccgggg atgacatgga atttcagagc ggtgccggcg
     3001 agctggatgg tatcgccgtc ggcaaccgcc atgtcgcgct tcggcggctc gccgaacagc
     3061 ggattgcgct tggtgccgaa caacggatcg cgctgggttt cctcgagcgc gttccagtcg
     3121 atcgcgctca tgacgatgcg cggattgaat ttttgcttga gatattgcgc gccgccataa
     3181 tgatcgccgt gggcatggct gatcacgaca tagcggatct cggcggggtc gagcccgagc
     3241 ttgcgcaggc cgccctcgat ataatgctcg gcctcgtcgg cattatccat ggcatcgacg
     3301 atgatgaggc cgccggcggt cttgatcgcc catgccgtgg tccatcgcga gcccagaaaa
     3361 tacaattcgt ccatcacctg catcggcgcc agcggcggct ccttcaccat ggcgcgataa
     3421 ttatccatga agctcgggcc cggcgcaggc gtcgcggttt tgcacagtcc aagcggcccg
     3481 agcagatccg acttcgcagc ggcggtggcg gtggcgatgt gttcctgcac cgcgacgggg
     3541 tcggcttttt gcgcgtgggt tggggaaatg ccggacaaga gagctatggc cgtgaccact
     3601 cccgcggacg cacgcccgaa cgcgatcttc atggttcctc ccaaatgtat gctttttttc
     3661 gtcatggccg ggcagaagcg cgtcttcgcg ctagatgtcc cggccatcca cgtttttttg
     3721 tcgcgacgtc cttaagacgt ggatgcccgg cgcaaggccg ggcatgacga caataacagc
     3781 ggtcacgcgt tgccgcggtc ctcgcgaaaa agatccagct tctgctgcac cgggcgatcc
     3841 gagaagctga acagcaccga atcctcatcc gcctcgtggg tgacccactg ccaactcggc
     3901 gccacgaaca ggtcgcgcgg gccccattca aacgtctgct cgccgatccg cgaacgccct
     3961 ttgccctcga tcgcggcgaa cacggtcgca tccgtcgcgc gatagcgcgc agttttaaaa
     4021 cccttcggaa gcaactggat gaaggtgccg atggtcggca tcgcgaaatc gccggtctcg
     4081 ggattggaga atttcagttt caggccgtgg caggcgtccc actcgccccg cacttttgcc
     4141 tgttccagcg cttcgcgggt gtagctgtag gggtaattga aaatcggcga ggttttcgaa
     4201 acgcgcttcc cgtcgaccgg cagcaaattg tggccgtaac gggcaaaact gtcgccggcg
     4261 ggccgggtga tcttctgctg gtcctcgttc gatccttccg cgaacgacgc atcgaagaac
     4321 tgcaccatcg ggatatcgag cccatcgagc cagaacatcg gctcggacgt ctcgttggaa
     4381 tggtcatgcc aggtcatcga cggcgtgatc acaaaatcgc cgggctccat cgcggtgcgc
     4441 tcgccatcca ccgcggtata ggcgcccttg ccttcaagga cgaagcgcag cgccgactgg
     4501 ctgtgccggt gcgccggcgc gacgtcgccg gggaccacca tctgcacgcc ggcaaacagc
     4561 gaggtagtga ttttcgactg cccgcgcagc ccgggatttt ccaggaccag cacccgccgc
     4621 tcggcttcct tggcggtgat cagtcggccg gcctccgtca tgtagtcgcg gatggcgtcg
     4681 aatttccaca agtgaggccg gcacgcgctc ttcggctccg gcgtgatcag gtcgcccatc
     4741 acgttccaca gcgccgacag attctcgccg tcgatcttct tgtagaacgc ctcgcgttcc
     4801 ggggtcttct gtacggcttc catatgagcc tcccatgtcc ttatcgtcgt tcttgttcgg
     4861 attattgaca gcatacttac aaagtggata gcgtcaatgc gcaatacaag caaaacgaga
     4921 agccaaaaag ggaggtttcc gatgaagctg cacggctatt tccgcagcag cgcgtcctac
     4981 cgggtcagaa tagcattgaa cctgaagggc ttgagcgcgg accacctgcc gcaccacctg
     5041 cgccgcggcg aacagcgcga tcccgcctat gtcgcgatca atccgcaagg cctggttccg
     5101 acgctgcagg acaatgcggg cgcgatcatc acccagtcgc tggccatcat cgaatggctg
     5161 gaggaaaccc atcccgaacc gccgctgttg ccgaccgatc cgctgcgccg cgccaaagtc
     5221 cgcgcctttg cgctggtgct ggcctgcgac acccaccccg tacagaacct gaaagtgctg
     5281 gcgcggctgc gcgagctggg tttgcccgag gatcaggtga cgggctgggc ggcctgggcc
     5341 aatcgtgaag gtctttcggc ttgcgaaacc ttgatcgcca acgagcccgg tccattctgc
     5401 tttggcgcga cgccgaccat ggccgatctc tgcctcgtgc cgcagttggc caatgcccgg
     5461 cgattcgggg tcgatctcaa cgcgttcccg cgcctgctga aggcggaaac agccgcgaaa
     5521 aacctcaagg cgttcgccga cgccgcgccg gacaaacaac cagatgccga gtaagaccaa
     5581 accgatcacg atggacgccg tctacaccgc accgggctac ctgttccggc ggatgcagca
     5641 gatcgcggtc gcgatcttcg tggaagaatg caaggcctac gacctgacgc cggtgcaata
     5701 tgcatcgctg gtcgcgatcc gcacccatcc cggcatcgac gcgacgcggc tctccgccgt
     5761 gatcgccttc gaccgctcga cgctcggcag cgtgatcgag cggctggagg ccaaggatta
     5821 tattgaacgc aagccggcgc gggaggacaa acgggtaaaa ctgctgcacc tgaccaggca
     5881 aggcgtggcg ctattgcgcg atatcatgcc gtcggttgat cgcgcccagg cgcggatgtt
     5941 gcagccgtta aagccggcgg accgcaagac gttgatggcg ctgctgacgc agctggtcga
     6001 tctcaacaat gaggcgtcgc gggtgccgct gcgcgccgaa gacgcgctcg aacatcttgg
     6061 gaaagcgaac tgatgggatc ggacgcgcgg cctgtcctga tcgcgggcgg cggcatcggc
     6121 ggcctggcgg cggcgcttgg cctcgcgcaa aaagggattc cctcgattct gctcgaaaag
     6181 gcatccgcgc tcggcgaaat cggcgccggc atccagctcg gccccaacgc gtttcatgcc
     6241 ttcgactatc tcggcgtcgg cgaggccgcg cggagcatgg cggtctatat cgatcagctg
     6301 cggctgatgg atgcgctcac cgccgacgag attacccatg tcgatctgcg cgacgcattc
     6361 cgctcgcgct tcggcaaccc ctacgcggtg gtccatcgtg gcgatctgca cggcgtattt
     6421 ctcagggcct gcgaggctca cgacctgatc gagctgcggg tcagcagcga agttttgggg
     6481 tatgaccagg atggctcttc cgtcaccgcg cggctggccg ccggcggcac cgttaccggc
     6541 tcgctgctga tcggcgcgga tggtttgtgg tcgaacatcc gaaaaaaagt caccgccgac
     6601 gggccgccgc gggtttccgg tcacaccacc tatcgttcgg tgattccgac cgaggagatg
     6661 ccggaggatc tgcgctggaa cgcggcgacg ctgtgggccg ggccgaaatg ccacatcgtg
     6721 cattatccgc tgtcgggctg gaaagtgttc aatctcgtcg tcacctgtca caacgacgcc
     6781 gcggagccgg tggcgggaaa accggtgtcc gacgccgagg tgatgcaggg tttcgcgcat
     6841 gtccatgcgc gcgcgcaaga tatcattcgc cacggcaaga actggcggct ctgggtgctg
     6901 tgcgatcgcg agcccaccga gcgctgggtc gacggccgcg tcgcgctgct cggcgacgcc
     6961 gcgcatccga tgctgcaata tttcgcgcaa ggcgcctgca tggcgctgga agacgcggtg
     7021 tgcctgtcgc acatgctggg cgcctgtccg gatgaccaca cgcgcgcgct ggagcagtat
     7081 cgttcgcagc gttttccccg caccgcgcgc gtacaactgt tatcccgcgc catcggcgag
     7141 cacatctatc atcccgcagg cgaacacgcc cgcatccgta atgccatcat gagcgcgaag
     7201 tcgtcggagg attattatcg cgatctcgcc tggctctatg g
//
LOCUS       DATNOI010000387         7264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_552476,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000387 DATNOI010000000
VERSION     DATNOI010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552476"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VN968_15340"
     CDS             <1..451
                     /locus_tag="VN968_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HXL30646.1"
                     /translation="LDDRILKRQVAVIDSMTRQERKNPDILKASRKKRIAAGAGLKVE
                     EVNKLLKMHRNMADMMKAMGSGKRGPMAGIAQAMGFGGGMPSPEQMKALAEKAPGGAG
                     PGGMPALPKDLPAGLRQGLPNLPGVTGLSGKPNLPGLGGFPGLGKKK"
     gene            597..983
                     /gene="rpsP"
                     /locus_tag="VN968_15345"
     CDS             597..983
                     /gene="rpsP"
                     /locus_tag="VN968_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HXL30647.1"
                     /translation="MSVVIRLARAGTKKRPVYHVVVADSRFPRDGRFIERLGHFNPLL
                     AKDNEARLKLDMDKTKAWLAKGAQPSDRVSRFLDAAGVAKRAARNNPEKAVPRKERKA
                     NAEAAATAATAAAAAAKTAAAAAAKK"
     gene            1002..1523
                     /gene="rimM"
                     /locus_tag="VN968_15350"
     CDS             1002..1523
                     /gene="rimM"
                     /locus_tag="VN968_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438991.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HXL30648.1"
                     /translation="MAAQICIARIGAAHGVRGAVKLWTFTEDPFAVKQYGPLLTKDGA
                     RSFEVATAREAKGHLVATLKGIATREEAERLNGLELYVAREKLPATDEDEYYHADLIG
                     LAAVTPADQPLGRVIAIHNFGAGDIIEIAPPQGSTMLLPFTNAVVPLVDIANGCVVIE
                     LPSEIDGGDEGSM"
     gene            1712..2407
                     /locus_tag="VN968_15355"
     CDS             1712..2407
                     /locus_tag="VN968_15355"
                     /inference="COORDINATES: protein motif:HMM:NF045041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30649.1"
                     /translation="MLRCVFLATIGLLATASSLPAQTVPNESPATASPPAVEAPGSPE
                     RMEDAQPGDHWTYELRDDITGDVKSTITNTVTDVSASEVSTRIAQLGNSNTGYQTFDL
                     SWNLMTNGIWRYTPNDGTGIRPPLAVGKTWSFKATDLNSTAGLSWKRSGTAKVIAQES
                     VTTRAGTFDTFKIETTLQIQNANDPTKKVQGVQQAWYAPVINHWVKRSFVSRSDGRVR
                     DKNTVELVEYGRR"
     gene            2469..3173
                     /locus_tag="VN968_15360"
     CDS             2469..3173
                     /locus_tag="VN968_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30650.1"
                     /translation="MLRYVVLVLVGLIASSASAQQAPPQAGAPAAPPSSPAPVEPAKA
                     VVPMEEPLPGDHWTYEVRDEITGKISATRANVVTEVTPTEISIRFEVVGTPNQGLNVY
                     DRSWNLINSGAWRYSPSDGTGIQKPLTTGKTWTFQANDVNAGGGYIWNRSGRSKVVGQ
                     ENLTTRAGTFETFKIETTYSRRNVKDPTKKEEVTGQTWYAPAIDHWVKRAVITRVDNH
                     LVVNNTLELTEYGRKQ"
     gene            3305..4039
                     /gene="trmD"
                     /locus_tag="VN968_15365"
     CDS             3305..4039
                     /gene="trmD"
                     /locus_tag="VN968_15365"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXL30651.1"
                     /translation="MTSIAPIWRATVLTLFPEMFPGPLGVSLAGKALASGLWALEARD
                     IRDSATDRHRSVDDTPAGGGPGMVLRADVLAAAIDAADAAPERPKLLMSPRGRPLTQS
                     RVAELAAGPGPLIVCGRFEGVDQRLIEARDLEEVSVGDYVLSGGEIASMALIDACVRL
                     LPGVMGKLASGADESFSEGLLEYPQYTRPQEFEGRTIPEILLSGDHVRVAAWRQAEAE
                     ALTRARRPDLWSAKASQKRPKNTTDG"
     gene            4155..4556
                     /gene="rplS"
                     /locus_tag="VN968_15370"
     CDS             4155..4556
                     /gene="rplS"
                     /locus_tag="VN968_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXL30652.1"
                     /translation="MNLIQELEKEQFDRLSAAGKPIPEFGPGDTVIVNVKVVEGDRSR
                     VQAYEGVCIGRSGGGLNESFTVRKISYGEGVERVFPVMSPMIDSITVVRRGKVRRAKL
                     YYLRNLRGKSARIVEKKTDRPQAAAAAAVGE"
     gene            4973..6379
                     /gene="leuC"
                     /locus_tag="VN968_15375"
     CDS             4973..6379
                     /gene="leuC"
                     /locus_tag="VN968_15375"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXL30653.1"
                     /translation="MSQPTTLYDKIWNDHLVHEADDGTCLLYIDRHLVHEVTSPQAFE
                     GLRTTGRKVRAPQKTLAVVDHNIPTTDRSKPNPDPESIEQMRVMAENAKEFGIEYYNE
                     FDKRQGIVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
                     IQKKAKNMRAVVDGQLPDGVTGKDIILAIIGEIGTAGGTGYVLEYAGEAIRALSMEGR
                     MTVCNMSIEGGARAGLIAPDEKAFEFLKGRPKSPKGEAWDAAMRYWETLRSDDGAHFD
                     HEIRLDAAKLPPIVTWGTSPEDVVSVTGMVPDPDKIDDEAKRLSKHRALNYMGLKAGT
                     KITDIKLDRVFIGSCTNGRIEDLRAAAKVVAGKTVNGNLNAMIVPGSGLVKEQAEAEG
                     LDKIFIKAGFEWREPGCSMCLAMNPDKLLPEERCASTSNRNFEGRQGFKGRTHLVSPA
                     MAAAAAIAGHFVDIREWR"
     gene            6481..6684
                     /locus_tag="VN968_15380"
     CDS             6481..6684
                     /locus_tag="VN968_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30654.1"
                     /translation="MADKPVFVDLISEATRQTRRRFAPDVDKPEVKERSRLSHWPPDR
                     WEQWRMETLTPWKLRAWKFKNWD"
     gene            complement(6774..>7264)
                     /locus_tag="VN968_15385"
     CDS             complement(6774..>7264)
                     /locus_tag="VN968_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30655.1"
                     /translation="ATDQRAEALSLISPGTAPAVTHASDALTTEVRGGHGGGGHGGGG
                     HGGFHGGGGGFHGGGAAFHGGGFHGGRAMFHGGGFHGGHVFHGGGFRHGGFAFRHHRF
                     HRHFYASYYDYPYYYSYHRCRVIWTYYGPRRICHYRHWHRYHYWRHHHYWHHHHRHHR
                     FY"
BASE COUNT         1493 a         2273 c         2282 g         1216 t
ORIGIN      
        1 gctcgacgac aggattttga agcgccaggt cgcggtgatc gattcgatga cgcggcagga
       61 acgcaagaac cccgatatcc tcaaggccag ccgcaagaag cgcatcgccg caggcgctgg
      121 cctcaaggtc gaggaagtca acaagctgtt aaagatgcac cggaacatgg ccgacatgat
      181 gaaggccatg ggctcgggca agcgcggccc gatggccggc attgcgcagg cgatgggctt
      241 tggcggcggc atgccctcgc ccgaacagat gaaggcgctc gccgagaagg cgcccggcgg
      301 tgcagggccg ggcggcatgc cggcgctgcc caaggatctt cccgcgggcc tgcgacaggg
      361 cctgccgaac ttgcctgggg tcaccggctt gagtggcaag ccgaatttac ctggcctcgg
      421 cggcttcccc ggcttgggga agaagaaatg aggaaagcgt cgtctctcag ctcgtcattc
      481 cgggatggtc cgcaggacca gacccggaat ctcgagattc cgggttcgcg cttcacgcgc
      541 cccggaatga cgagccagtt gaatcaacat tctttcgaac tcacaggaga acctaaatgt
      601 cagtcgttat ccgccttgct cgcgcaggca ccaagaagcg cccggtctat cacgtcgtcg
      661 tcgccgactc gcgctttccg cgcgacggcc gtttcatcga acggctcggt catttcaacc
      721 cgctgctggc gaaggacaat gaggcccggc tgaagctcga catggacaag accaaggcct
      781 ggctcgccaa gggcgcgcag ccgtcggatc gcgtgtcgcg gttcctcgat gccgccggcg
      841 tggcaaaacg cgccgcgcgc aacaatccgg aaaaggccgt gccgcgcaag gaacgcaagg
      901 ccaacgccga agccgccgcc acggctgcaa cggccgcggc agcagccgcc aagacggcag
      961 cagcggcagc agccaagaag taagcgcggc ggcgcgtggt gatggcggcg cagatctgca
     1021 tcgcccggat cggcgccgcg catggcgtgc gcggcgcggt gaagctgtgg acgtttaccg
     1081 aagatccgtt tgccgtgaag caatacgggc cactgctgac caaagacggc gcgcgctcgt
     1141 tcgaagttgc gaccgcccgc gaggcaaaag gccacttggt tgcgacgctg aaaggcattg
     1201 cgacgcgcga ggaggccgag cgcctcaatg ggcttgagct ttacgtcgcg cgcgaaaaac
     1261 tgccggccac cgacgaggat gagtactatc acgccgacct gatcgggctc gccgcggtaa
     1321 cgcccgcgga tcaaccgctc ggccgcgtca tcgcgatcca caatttcggc gccggcgaca
     1381 tcatcgagat cgcgccaccg cagggctcga ccatgctgct gccgttcacc aacgccgtgg
     1441 tgccgttagt cgatatcgcg aacggatgtg tggtgatcga actgccttcg gagattgacg
     1501 gcggagacga aggatcgatg taggttccgc tcatcgctgg cttcgcgtat cgcgcccaac
     1561 actaaaatta tgacgttaaa attaagacag actgcagctg aaaattacaa aatacttgcg
     1621 ttcaattttc ggctcaggca tttttggtgc ctcgtcctgc ggcaacccgt tcgcgatttg
     1681 ctgcccgatg ctacagcaca cgagatgttc catgttgcgt tgcgttttcc tcgcgaccat
     1741 tggcctgctc gcaacagcga gttctctgcc ggcccaaacg gtgcccaacg agtccccagc
     1801 cacggcgtcc ccgcccgctg ttgaagcacc cggtagcccc gagcgcatgg aagatgcgca
     1861 gcccggcgac cattggacct acgagcttcg ggacgacatt accggcgatg tcaaatcgac
     1921 aattaccaat accgtgaccg acgtctcggc ttccgaggtc agcacccgga ttgcccagct
     1981 cggcaattcc aacacgggct atcagacctt cgatctttcc tggaatctga tgaccaacgg
     2041 catttggcgg tatacgccca atgacggcac cggcattcga ccgccgctgg cggtgggcaa
     2101 aacctggtcg ttcaaagcga ccgatctcaa cagtacggcc ggacttagct ggaagcgctc
     2161 gggcaccgcg aaggtgatcg cgcaggaaag cgtcacgacc agagcgggca cgttcgacac
     2221 cttcaaaatc gagacgaccc ttcaaataca aaatgcgaat gacccgacca agaaagtaca
     2281 gggcgtgcag caggcctggt acgcgccggt gatcaatcat tgggtgaagc ggtcattcgt
     2341 atcgcggtca gacggacgcg ttcgggataa gaacaccgta gagctagtcg agtatggccg
     2401 tcgctagttg cgccggcaca cgcgcagcgg ccctcgcagg aataacaagc ggcatgtagg
     2461 agacgccaat gcttcgttac gtcgttctgg tgctggtcgg cctgatcgcc agcagcgcgt
     2521 cggcgcaaca ggctccaccg caagccggag cgccggcggc tccgccgtcg tcgcccgccc
     2581 cggtcgagcc tgcaaaagca gttgttccga tggaggagcc gttgcccggc gaccattgga
     2641 cctacgaggt acgagacgaa attaccggga aaatcagcgc cacccgcgcc aatgtcgtta
     2701 cggaggtgac gccgaccgaa attagcatcc gcttcgaggt cgtgggcacc cccaaccaag
     2761 gcctcaacgt ttatgaccga tcctggaatt tgataaactc aggtgcctgg cgatactcgc
     2821 cgagcgacgg cacgggaatc caaaagccgc tcacgaccgg caagacgtgg acttttcaag
     2881 ccaacgacgt caatgccggg ggtggataca tctggaaccg atcgggccgt tcaaaagtcg
     2941 tcggccagga aaacctgacc accagggccg gtacgttcga gacgttcaag atcgaaacta
     3001 cctattcgag gcgcaacgtc aaagacccga ccaagaagga agaggtgaca ggacagactt
     3061 ggtacgcgcc tgcgatcgat cattgggtca agcgcgccgt tatcactcgg gtcgacaatc
     3121 atctggtcgt caacaatacc ctcgaactta ccgaatatgg tcgtaagcaa tgagacccgg
     3181 acgccgccat aagatcaata ctcccgggtt ggtgccgcta gtctcacccg cgcgccgatg
     3241 ccgatgttcc gaggaagcgc cgcagccggt ttgacggccc cggccggacg cgccattgga
     3301 aggcatgaca tccatagctc ccatctggcg cgcgaccgtg ctgacgctgt tcccggagat
     3361 gttcccgggc ccgctcggcg tcagtcttgc gggcaaggcg ctggcgtccg ggctttgggc
     3421 gctggaggcg cgcgacatca gggattccgc caccgaccgg catcgcagcg tcgacgatac
     3481 ccccgccggt ggcggccccg gcatggtgct gcgcgccgac gtgttggcgg ctgcgatcga
     3541 tgcggcggat gcagcgccgg agcgtccaaa actgctgatg agtccgcggg gtcggccatt
     3601 gacccagtcg cgggtcgcgg aacttgcggc cggacccggg ccactgatcg tctgcggccg
     3661 gttcgagggc gtcgaccagc gcctgatcga ggcccgcgac ctcgaggaag tttcggttgg
     3721 cgactacgtg ctttcggggg gtgaaatcgc cagcatggcg ctgatcgatg cgtgcgtccg
     3781 gctgttgccg ggcgtgatgg gcaaactcgc ttccggtgct gacgaaagct tttcagaggg
     3841 cctattggaa tacccgcaat acacccgccc gcaggagttc gagggccgca ccatcccgga
     3901 aatcctgttg tccggcgacc atgtccgggt cgcggcctgg cgccaggccg aggccgaggc
     3961 cctgacccgg gcgcggcggc ccgatctctg gtccgcaaaa gccagccaaa aacgcccaaa
     4021 aaacacgacg gatgggtgac aagccgcggc ctttgccgta tacgagcgcg aaatccgcgc
     4081 aatggcagga taaatggacg ttcgcgcagc ccgcgcctcg caggctgggg cgcgccgccg
     4141 atggagattt tgcgatgaac ctcattcaag agctcgaaaa agagcaattc gaccgactgt
     4201 ccgcggccgg caagcccatt ccggaattcg gtccgggcga caccgtgatc gtcaacgtca
     4261 aggtggtcga aggcgaccgc tcacgcgtgc aggcctatga gggcgtgtgc atcggccgct
     4321 ccggcggcgg gctcaatgag agctttacag tgcgcaagat ttcctacggc gaaggcgtgg
     4381 aacgcgtatt ccccgtgatg tcgccgatga tcgattcgat caccgtggtg cgccgcggca
     4441 aggtacgccg cgccaaactg tattacctgc gcaacctgcg cggcaagtcg gcccgcatcg
     4501 tcgaaaagaa gacggaccgc ccgcaggccg ccgctgctgc cgccgtcggc gagtaattct
     4561 tccgtcccga atggatttga cgaaaagcgc gggctcaccc ccgcgctttt tttgttgcgc
     4621 cgagtcgtca ttgcgagcca acggatcgcg cgaatgcgcg cccgatgaca ggctccgcga
     4681 agccatccat cttttgcggt agcgaagggt ggattgcttc gtcgcaagtg cgcaatgacg
     4741 gtggcggcaa acgcatatcg cgcttagata cctgatgtgc tatagggccg gaatgttcag
     4801 gtatcgccga accatcgcgt ctcgcgtcgt tctcgacgat cgcagtcgtt tgccttggcg
     4861 tttcttcggt cgcctcacga cggccgccct cgcggcttga cccgtagcgc gccgcattgg
     4921 ccgcgcgctg ttcgcatttt ccgattttaa tctcgattca aggcctgaca ccatgtccca
     4981 accgaccacg ctgtacgaca agatctggaa cgatcatctg gttcacgaag ccgacgacgg
     5041 cacctgcctg ctctatatcg accgtcatct ggtgcacgag gtgacctcgc cgcaggcctt
     5101 cgagggtctg cgcaccaccg gccgcaaggt tcgcgcgccg cagaagacgc tggccgtggt
     5161 cgatcacaac atcccgacca ccgaccgctc aaagcccaat ccggatccgg aaagcatcga
     5221 acagatgcgg gtgatggccg agaacgccaa agagttcggc atcgaatatt acaacgagtt
     5281 cgacaagcgt cagggcatcg tgcacgtcat cggccccgag caaggcttca cgctgcccgg
     5341 caccaccatt gtctgcggcg acagccacac ctccacccat ggcgccttcg gcgcgctggc
     5401 gcacggcatc ggcacctccg aagtcgagca cgtgctggcg acgcaaaccc tgatccagaa
     5461 gaaggcgaaa aacatgcgcg ccgtcgtcga cgggcaattg cccgacggcg tcaccggcaa
     5521 ggacatcatt ctggcgatca tcggcgagat cggcaccgcc ggcggcaccg gctatgtgct
     5581 ggaatatgcc ggcgaggcga tccgcgcgct gtccatggaa ggccgcatga cggtctgcaa
     5641 catgtcgatc gaaggcggcg cccgcgccgg cctgatcgcg cctgacgaaa aagcctttga
     5701 gtttctaaaa ggccgcccga agtcgccgaa gggcgaggcc tgggacgccg cgatgcgcta
     5761 ttgggagacg ctgcgctctg acgacggcgc gcatttcgat cacgagatca ggctcgacgc
     5821 ggcaaagctg ccgccgatcg tgacctgggg cacctcgccc gaagacgtgg tgtccgtcac
     5881 cggcatggtg ccggatcccg acaagattga tgacgaggca aaacggctgt caaaacatcg
     5941 cgcgctgaac tacatgggct tgaaggccgg caccaaaatc accgacatca agctcgatcg
     6001 cgtcttcatc ggctcctgca ccaatggccg cattgaggat ttgcgcgcgg ccgcgaaggt
     6061 ggtggccggc aagaccgtca acggcaatct caatgcgatg atcgtgccgg gctcaggcct
     6121 tgtgaaggag caggccgaag ccgaaggcct cgacaagatt ttcatcaagg ccggcttcga
     6181 atggcgcgag ccgggctgct cgatgtgtct ggcaatgaac cccgacaagc tattgccgga
     6241 agagcgctgc gcctcgacct cgaaccgcaa tttcgagggc cgccaaggtt ttaaaggccg
     6301 cacccatctg gtgtcgccgg cgatggcggc ggcggcggcg atcgccgggc attttgtcga
     6361 tatcagggag tggcggtagg cacctcttgc ctcaacgtcg aggcattccc gaagggagtc
     6421 cttaagcagt tgggagcaga ctgatccttg cgaaggcgag ccgtttcgca ggagcgggcc
     6481 atggccgaca agccggtttt cgtcgatctg atcagcgagg cgacgcgcca gacgcggcgc
     6541 cggttcgcgc ctgacgtcga caagcccgag gtcaaggaaa ggtcgcgtct cagccactgg
     6601 ccgcccgacc gctgggagca gtggcgcatg gagacgctga ccccctggaa gctcagagcc
     6661 tggaaattca agaactggga ttgaagggcg gcaaagtttc ttcgcggcag acacacatca
     6721 ggcgcccaaa agggcgcctg attggtttcg acccgccgga cttccaaacc tcatcaatag
     6781 aagcgatgat gccggtgatg gtgatgccag tagtggtgat gacgccagta gtggtagcga
     6841 tgccagtggc gatagtggca gatcctgcgc gggccataat aggtccagat cacccggcag
     6901 cggtggtacg aataataata cgggtagtcg taataggacg cgtagaagtg ccgatgaaag
     6961 cggtggtgcc gaaacgcgaa cccgccgtgg cgaaagccgc cgccgtggaa aacatggccg
     7021 ccgtgaaagc cgccgccatg gaacatggcc ctaccgccgt ggaaaccgcc accgtgaaat
     7081 gccgcgcctc caccatggaa gccgccgcca ccaccgtgaa atccaccatg ccccccaccg
     7141 ccatggccgc caccgccatg gccgccgcgg acttccgtcg tcagcgcgtc ggaggcgtgc
     7201 gtcaccgccg gcgccgtgcc cggactgatc agcgacagcg cttccgcgcg ctggtcggtt
     7261 gcgg
//
LOCUS       DATNOI010000388        11468 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_547961,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000388 DATNOI010000000
VERSION     DATNOI010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11468
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547961"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VN968_15390"
     CDS             complement(<1..533)
                     /locus_tag="VN968_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL30656.1"
                     /translation="MADPLLRVEKLVRRFGGVIATDHVSLDVGQGELHAIIGPNGAGK
                     TTLISQLTGQLPPNSGTIHFAGADITRLPAFRRSALGLARSFQITSLLPDFTASDNVA
                     LAAQAHDGHSFYFWGNARKESALRNAALAALKRVGLEHRADVVVSRLSHGEQRELELA
                     VALATKPQLLLLDEPMAG"
     gene            complement(526..1473)
                     /locus_tag="VN968_15395"
     CDS             complement(526..1473)
                     /locus_tag="VN968_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL30657.1"
                     /translation="MIASLNARNAIAALVVAGLLLVPLYTSMSGNIFVLTLFTRIVIF
                     ALAAASLNLIMGYGGMMSFGHAAYLGIGGYAVGILAHEGIGSGLIQWPVALAASALFA
                     LVIGALSLRTRGVYFIMITLAFAQMAYYVASGLARYGGDDGLTIYKRSDFGGLINLSS
                     RVQFYYLCLACLLGGIYLIWRIVNSRFGMVVQGLRSNEQRMQAIGFPANRYRLVCFVI
                     AGTMCGLAGALLANNTDFVSPAVMYWTRSGDLMVMVILGGMGSLFGPVIGAIVYLLLE
                     EALSQVTEYWALIMGPLLLLIVLFGRGGIMGLLGRLSRG"
     gene            complement(1470..2387)
                     /locus_tag="VN968_15400"
     CDS             complement(1470..2387)
                     /locus_tag="VN968_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL30658.1"
                     /translation="MLLVVEQFLNGLQFGLLLFLLAAGLTLVFGIMDFVNLAHGSLYM
                     MGAYFAATFVAWTGNFVIGVVLALGATLLLGIVLEFVALRHLYGRDHLDHVLATFGLI
                     LFFNDAVRLIWGPAGLALPLPAWLTVPIQIMPGVYYPAYRLAIIVVALAVALLLYVVV
                     MRTRLGMLIRAGASNREMIGALGINIKLLYTLVFGLGAALAGLAGLMQAPILTVQIGM
                     GENILILAFVIIVIGGIGSIRGAFLAAIFVGLIDTLGRAFLPDLLRQILSSTAASTAA
                     PALSSMLIYLLMAMVLVVRPEGLFPANRR"
     gene            complement(2643..3815)
                     /locus_tag="VN968_15405"
     CDS             complement(2643..3815)
                     /locus_tag="VN968_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30659.1"
                     /translation="MRTAFWLAGATVLALANPAVAADTIKIGFVSTFSGPTAVIGNDM
                     RNSFELALDHMGRKMGGLPVEVIYEDDQQKPDVGKQKTEKLIQSDKVDFIAGYIWSNV
                     LLASLKTAVDAQTFLISANAGPSQLAGELCSPYVFSTSWQNDQTPQAVGLYMNQKGVK
                     SVFLIGPNYAAGKDMLAGVKSTFKGEVKGEEYTVWPSQLDFSAELSKARASGAESIFV
                     FYPGAAGVQFLNQYAQAGIKAQIPLYTAFTIDELSLPLQKENALGVPGAQEWVNDLPN
                     EENKRFVADYRKKYPNLRPTFYGAQSYDAAQLINSAVVAVKGDTSKKDAMKAAMEKAN
                     FKSVRGPFKFGNNHIPIQNFYLQDVVKDAEGQLSLKTVATIVENSQDRFADKCPMK"
     gene            complement(4132..5016)
                     /locus_tag="VN968_15410"
     CDS             complement(4132..5016)
                     /locus_tag="VN968_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXL30660.1"
                     /translation="MVPEAPAAAADIDGLLARMRPKLHRYCARMVGSVIDGEDVLQDA
                     LIKAVEALASVGPVGNPEGWLFRIAHNTALDFLRRRNRQQALRSAEEVDMIADEADTV
                     ASRQIASASLRTFMRLPVAQRASVILMDVLGCSLQEICEAMDFSLPAVKAALHRGRAQ
                     LRELADEPEDRPQPKLSDADRDRLGAYVAHFNARDFDAIRAMIADDVRLDLVSKTRLN
                     GKAEVSRYFGNYSGINDWHLVPGLVEGRPAILVFDPNDPGSGPKYFMLLQWSAGKVAD
                     IRDFRHASYVIDGAEYLI"
     gene            complement(5052..5357)
                     /locus_tag="VN968_15415"
     CDS             complement(5052..5357)
                     /locus_tag="VN968_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30661.1"
                     /translation="MKRTLIRYKTKPELADKNAELISGVFAELKAAKPEGVRYLSLRL
                     EDDTFVHFVETESDSANALPGLAAFKAFQSGIRDRCIEPPLAGSVTIVGNYRMLREP"
     gene            5368..5613
                     /locus_tag="VN968_15420"
     CDS             5368..5613
                     /locus_tag="VN968_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30662.1"
                     /translation="MAGFAMRNHRVSADRRLATAEGFGRKEIIRALSADADIPDDAIL
                     ISPDFARIELDLGPWLELQKDYMSTERSESEVKGRLT"
     gene            5610..6893
                     /locus_tag="VN968_15425"
     CDS             5610..6893
                     /locus_tag="VN968_15425"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL30663.1"
                     /translation="MTEAQARSAQPHRVVIVGAGFGGLEAAFRLSGTPVSITLIDRRN
                     HHLFQPLLYQVATASLATSEIAWPIRYLLRDRPDVTTLYATVTGVDAAAKRVLLDDGD
                     GLPYDTLILATGARHAYFGHDEWEPFAPGLKTLEDATTLRRRILVAFERAERETDPQR
                     REASLTFVIIGAGPTGVELAGTIAELAQDTLPPDFRNIDTHKARVVLIEAGPRVLAGF
                     ADDLSAYAQRSLESIGVEVKLGQAVTECTADSVVYGGNRLDTKTIIWAAGVRASPAAE
                     WLGAPADRAGRLQVLPDLTVPNHPDIFAIGDTVVINGPDGKPVPGIAPAAKQQGHYVA
                     DAIKARLSGGTPALFRYQHAGSLAQIGKRKAVIDFGRIKLRGTLAWWIWGIAHIYFLI
                     GLRNRLSVAISWLWVYVRDQRAARLITQGSSKVVR"
     gene            complement(7024..8238)
                     /locus_tag="VN968_15430"
     CDS             complement(7024..8238)
                     /locus_tag="VN968_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30664.1"
                     /translation="MRDFRLYLALALSCAAWGSARAEISGDVVRVGVLNDISGIFQDT
                     NGMGSVEAARMAAEDFNGGGKNIKVEIVYADHQNKADVGSAIARKWLDVDGVDAIVDV
                     PNSAVGLTINSLVRDSRMTFLASSTASSDLTGKACSPNTIQWVNDAWATGNTTAAAMM
                     ARGGKDWYFVTVNYALGQGIEAEATNYIEKHGGKVLGSAKHPLGTSDFASFLLQAQNS
                     KAKVIGLANAGGDTINAVKQAAEFGIQQNGQTMVAFLLFVNDVHGMGLKVAQGLQLLE
                     AFYWDMDDDTRAFAKRFAARPGINGKMPSGNQAGVYASTLAYLNAVAATGSDAAKDVV
                     PQMKKFKGKDKLFGEVTIRQDGRVIHPMYLFEVKKPDESKYPYDYYRLVSTIPADQAF
                     RPIADGGCSLVK"
     gene            complement(8326..8649)
                     /locus_tag="VN968_15435"
     CDS             complement(8326..8649)
                     /locus_tag="VN968_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXL30665.1"
                     /translation="MPIITFIRSDNRSESIEARIGDSAMLAATRHGVDEIVAECGGNA
                     MCATCHVYVDDNWIGRLAAMSDEEDALLDGVAAERQPNSRLSCQIKIEQELDGLVLRL
                     PERQL"
     gene            complement(8655..9905)
                     /locus_tag="VN968_15440"
     CDS             complement(8655..9905)
                     /locus_tag="VN968_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXL30666.1"
                     /translation="MVTTGSTAVGGGQAGAASGGVPSGIPGLDVDPFAIEFFENPFPT
                     HEALREAGPLVHLDKWNVYAVARYAEVHRVLNDPVTFCSSRGVGLSDFAKEKPWRPPS
                     LILEADPPAHTRTRAVLSRVLSPAVMKQIRDGFAAAAEAKVDELLAKGSFDAVTDLAE
                     AYPLSVFPDAVGLKQEGREHLLPYAGVVFNAFGPPNQLRQTAIERSQPHQAYVQEQCQ
                     RDNLAPGGFGACIHARVDAGDIAAAEAPMLVRSLLSAGIDTTVNGIGAAIYCLARFPE
                     EFARLRSDPSLARNAFEEAIRFESPVQTFFRTTTKDVEISGCKVGAGEKILMFLGAAN
                     RDPRRWADPDRYDITRKTSGHVGFGSGIHMCVGQLVARLEGEVMLAALARKVAAIEIT
                     GPVKRRYNNTLRGLETLPITLRPV"
     gene            9988..10515
                     /locus_tag="VN968_15445"
     CDS             9988..10515
                     /locus_tag="VN968_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXL30667.1"
                     /translation="MSKNGSTGSRRKAALASGVKPGQTLDLERYVPAFVTFIANKLSR
                     SATALYQKRFGVNVTEWRIMSLLAIEPGITASRICYVIGFDKGPVSRTLALMQKRGLI
                     AIKTDPRDGRSHSISLTPKGRKVHDQVIVAALERERRLLACLRKDEREVLIDLLRRVH
                     SNLDAVTDHGEPGED"
     gene            complement(10831..>11468)
                     /locus_tag="VN968_15450"
     CDS             complement(10831..>11468)
                     /locus_tag="VN968_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HXL30668.1"
                     /translation="KLVPSGDKLEVRVRGPNVTPGYWKAPELTAQAFDADGFYLIGDA
                     VTFADPERPELGLFFDGRVAEDFKLNSGTWVSVGTLRVAGITALAPLAQDIVVSGHGG
                     DHVRFLVFPNLSACRVHAGLPESSDVSEVIGNEKVRAAIASGLAKLRAQGGGSSGHAA
                     RALLLAEPASVDGGEITDKGYINQRAVLTRRASAVAILEDDASTAWIGCPG"
BASE COUNT         2228 a         3681 c         3500 g         2059 t
ORIGIN      
        1 ccggccatcg gttcgtcgag cagcaacaat tgcggcttgg tcgcaagtgc tacggccagc
       61 tcgagttcgc gctgctcgcc atgactcaac cgggaaacca cgacgtcggc gcggtgttcg
      121 agcccgaccc tcttcagggc tgccagcgcc gcgttgcgga gcgcgctttc cttgcgagcg
      181 ttgccccaga agtagaacga gtgcccatca tgggcctgtg ccgccagcgc gacattgtcc
      241 gatgcggtga agtccggcag cagcgaggtg atctggaatg agcgcgccag cccgagcgcg
      301 ctgcggcgaa acgccggcag gcgggtgata tccgctccgg caaaatgaat ggtgccggaa
      361 ttcggcggca gctgtccggt gagttggctg atcagcgtgg tcttgccggc gccgttcggt
      421 ccgatgatcg cgtgcagttc gccttgtccg acatcgagcg agacatggtc ggtcgcgatc
      481 acgccgccga accgccgcac caatttttca acccgaagca agggatcagc cacggctcag
      541 cctcccgagc agccccataa tgccgccgcg cccgaacagc acgatcagca gcagcagcgg
      601 gcccatgatc agcgcccagt attccgtcac ctgcgataac gcctcttcca gcagcagata
      661 gacgatcgcg ccgatcaccg gaccgaacag cgagcccatg ccgccgagga tgaccatcac
      721 catgaggtca ccggaacggg tccagtacat caccgccgga ctgacaaagt ccgtgttgtt
      781 cgcgagcagc gcaccggcga ggccacacat ggtgccggcg attacaaagc agaccagccg
      841 gtagcggttg gcgggaaacc cgatcgcctg catgcgctgc tcgttggaac gcaggccctg
      901 aacgaccatg ccgaagcgcg aattgacgat ccgccagatc agatagattc cgcccaacag
      961 acaggccaga caaagataat agaactgcac ccggctcgac aggttgatga gcccgccgaa
     1021 atcgctgcgc ttgtagatcg tgagcccatc gtcgccgccg tagcgcgcaa gcccggatgc
     1081 gacgtaatac gccatctgcg cgaaggccag cgtaatcatg atgaaataga cgccgcgggt
     1141 gcgcaagctg agcgcgccga tcacgagcgc gaacagcgcc gacgccgcca gcgccaccgg
     1201 ccactggatc aatcccgagc cgatgccttc gtgcgcgaga atgccgaccg catagccgcc
     1261 gattccaaga taggcggcgt gaccgaagct catcatgccg ccatagccca tgatgagatt
     1321 aaggcttgcc gccgccagcg cgaagatcac gatgcgggtg aacagcgtca gcacgaagat
     1381 gtttccactc atcgaggtat aaagcggaac caacaacagg ccggcaacca ccagcgccgc
     1441 aatcgcgttg cgggcgttga gcgaagcaat catcgacggt tggccggaaa cagcccctct
     1501 ggccgcacca ccaacaccat ggccatcaac agatagatca gcatcgagga gagcgcgggc
     1561 gccgcggtgg aagccgcggt cgagctcagg atctgccgca gcaggtcggg caggaacgcg
     1621 cggcccagcg tgtcgatcaa gccgacgaag atcgcggcca ggaatgcgcc gcggatcgag
     1681 ccgatgccgc cgatcacgat gatgacgaaa gcaaggatca ggatgttctc gcccatcccg
     1741 atctgaacgg tgaggatcgg cgcctgcatc agcccggcga ggccggcgag tgcggcgccg
     1801 agcccgaaca ccaaggtgta gagcagcttg atgttgatgc cgagcgcgcc gatcatttcc
     1861 cggttggagg cgccggcgcg gatcagcatg ccgagccggg tgcgcatcac gacgacataa
     1921 agcagcaggg cgacggcgag ggcgaccacg atgatcgcca gccggtaggc gggataatag
     1981 acgccgggca tgatctggat cggcaccgtc agccaggccg gcagcggcag cgcgagaccc
     2041 gccggacccc agatcagccg caccgcgtca ttgaagaaca ggatcaaccc gaatgtcgcc
     2101 agcacatgat cgagatgatc gcggccgtag aggtgccgca gtgcgacgaa ttccagcacg
     2161 atgccgagca gcagcgtcgc cccaagcgcc agcaccaccc cgatgacgaa atttcccgtc
     2221 cacgccacga aggtggccgc aaaataggcg cccatcatgt agagcgagcc atgcgccaga
     2281 ttgacgaagt ccatgatgcc gaacaccagc gtcagccccg ccgccagcag aaacagcagc
     2341 agcccaaact gcaatccgtt caggaattgt tcgacgacaa gcagcatgcg cgttctaggt
     2401 cacttccggt tgaggcgtac aacaatgggc atcgaacggg cctcatggtt cgagacgcgc
     2461 ggcgttgccg cgctcctcac catgagtgtt tagagcacct catcctgagg agcatcgcga
     2521 agcgatgcgt ctcgaaggat gaagccgcga gactggaagt catcgaggtg caagacatgt
     2581 gccggaaagg gaaaacagcg gcagcgcgcg agcgccgccg ccgtatcgga taggtacccg
     2641 gatcacttca tcgggcattt gtcggcgaag cgatcctggc tgttctcgac gatggtggcg
     2701 acggttttga gcgagagttg accttcggcg tccttgacca cgtcctgcag atagaaattc
     2761 tggatcggga tgtggttgtt gccgaacttg aacggaccgc gcaccgactt gaaattggct
     2821 ttctccatcg ccgccttcat cgcgtccttt ttgctggtgt cgcccttcac agcaaccacc
     2881 gcgctgttga tcagttgcgc ggcatcatag gattgtgcgc cgtaaaaggt cgggcgcagg
     2941 ttggggtact tcttgcggta gtcggcgacg aaccgcttgt tctcttcgtt cggcaggtcg
     3001 ttcacccact cctgcgcgcc gggaacgccg agcgcatttt ccttctgcag aggcagcgac
     3061 aattcgtcga tggtaaaggc ggtgtagagc gggatctgcg ccttgatccc ggcctgggca
     3121 tattgattga ggaactggac gccggccgca cccggataga acacgaagat cgactccgca
     3181 cctgaagcgc gcgccttgga caattcggcc gagaaatcga gctggctcgg ccacaccgta
     3241 tattcctcgc ccttgacctc gcccttgaac gtgctcttga cgccggccag catgtccttg
     3301 ccggcggcat agttcggccc gatcaggaac actgacttga cgcccttctg attcatgtaa
     3361 aggcccacgg cctgcggggt ctggtcgttt tgccaggatg tcgagaacac gtaaggcgag
     3421 cacagctcgc cggcgagctg cgacggcccg gcgttggcgc tgatcaggaa ggtctgcgca
     3481 tcgaccgcgg tcttgagcga ggccaagagc acgttcgacc agatatagcc ggcgatgaag
     3541 tcgaccttgt cggactggat cagcttctcg gtcttctgct tgccgacatc gggcttctgc
     3601 tggtcatcct cgtagatcac ctcgaccggc agcccgccca tcttgcggcc catgtggtcg
     3661 agcgccagct cgaacgaatt gcgcatgtcg ttgccgatca ccgcggtcgg gccgctgaag
     3721 gtagagacaa acccgatctt gatggtgtca gccgcgaccg cgggatttgc cagcgccaga
     3781 accgtcgcgc ccgccagcca gaatgccgtt ctcatcgtta ctccccctcg tcattaaatt
     3841 aaatacgccg cagtcaatcg ccctgcccgg ttcggggagc cttctttgcc ccttttttcc
     3901 gttttcgagc cgctccgatt gatggcgagc tttgtgcgcg aaatcttagg cttgcggcaa
     3961 gcacgacctt cggtgtccgt taacatcaaa ggctggcttg caaagatcat gcaccataat
     4021 tcgtacaggg cgcgaatagc aagaattata gttctggtta ttaactggcg cgcgtggcga
     4081 tgccccggcc ggccgcaaca tgctgacagg tagcctgggc cggcaggcgg atcagatcag
     4141 gtattccgcg ccatcgatga cgtaggacgc atggcggaaa tcgcggatat cggcgacctt
     4201 gccggccgac cattgcagca gcatgaaata ttttggcccc gagcccgggt cgttgggatc
     4261 gaacaccagg atggccggcc gtccttcgac cagcccgggc accagatgcc aatcgttgat
     4321 cccagaataa tttccgaagt atctggaaac ctcggccttg ccgttcaagc gggtcttgct
     4381 gacgaggtcg aggcgtacat cgtccgcgat catggcgcgg atcgcgtcga aatcgcgcgc
     4441 gttgaaatgc gcgacatagg cgccgaggcg atcgcgatcg gcgtccgaca atttcggttg
     4501 cggccgatcc tcgggctcgt ccgccagttc gcggagctga gcgcgtccgc gatggagggc
     4561 tgctttcacc gcgggcaggc tgaaatccat cgcctcgcaa atctcctgaa gcgagcagcc
     4621 gagcacatcc atcaggatca cgctcgcgcg ctgggcgacc ggaagccgca tgaaggtgcg
     4681 aagactggcg ctggcgatct ggcggctggc cacggtgtcg gcttcatcgg caatcatgtc
     4741 cacctcctcg gccgatcgaa gcgcttgctg gcggttgcgg cggcgcagga aatccagcgc
     4801 ggtgttgtgc gcgatccgga acagccagcc ttccgggttg ccgaccgggc ccactgaagc
     4861 aagcgcctct accgccttga tcagcgcatc ctgcagcacg tcctcgccgt cgatcaccga
     4921 gccgaccatg cgcgcgcaat agcgatgcag ttttgggcgc atcctggcga ggaggccgtc
     4981 aatatcagcg gcagcggccg gtgcctcagg gaccaatttc attcagcctc cgaacaatgc
     5041 gagcccgacg ctcagggctc acgcagcatc cgataattgc cgacgattgt aacgctgccg
     5101 gcgagcggtg gttcaatgca gcgatcgcga atcccgctct ggaatgcctt gaaagcagcc
     5161 agtcccggca aggcattcgc ggaatcggat tcggtctcga cgaaatgaac aaaggtatca
     5221 tcctcgagcc gcagcgaaag atagcgtacg ccctcgggtt tcgcggcttt caattccgcg
     5281 aacacgcctg agatcagctc ggcattcttg tcagccaact ccggcttggt cttatacctg
     5341 atcaaggtcc gtttcatcat tgcctccatg gcaggttttg cgatgcgaaa tcatcgcgtc
     5401 tcagccgata gacgtttggc gacggcagaa ggattcggcc gcaaagaaat tattcgcgct
     5461 ctcagtgctg acgctgacat accggacgat gcgattttga tatcgcccga ttttgcaagg
     5521 attgaactag atctggggcc ttggctcgaa cttcagaagg attacatgtc aaccgaacgt
     5581 tccgagtctg aagtcaaagg ccgcctcaca tgaccgaagc gcaggcgcga tccgcacaac
     5641 ctcatcgcgt ggtgatcgtc ggggccgggt tcggcgggct ggaagcggcg tttcggctct
     5701 cgggcacgcc ggtcagcatc acgctgatcg accgtcgcaa ccaccatcta ttccagccgc
     5761 tgttgtatca agtcgccacc gcgtcattgg caacgtcgga gatcgcctgg ccgatccgct
     5821 atctgttgcg cgatcgcccg gacgtcacga ccctgtacgc caccgtgacc ggggtggatg
     5881 cggcagccaa gcgcgtactg ctcgatgatg gcgacggcct gccatacgac acgctgattc
     5941 tcgccactgg tgcgcgtcac gcgtatttcg gccatgacga atgggagccg ttcgcgcccg
     6001 gcctgaaaac actcgaggac gccaccacgc tgcggcggcg cattctggtg gccttcgagc
     6061 gcgccgagcg cgaaaccgat ccgcagcggc gtgaggcctc gctgactttt gtgattatcg
     6121 gcgccggccc gaccggggtc gaactcgccg gcaccatcgc cgagctggcg caggacacgc
     6181 tgccgcccga cttccgcaac atcgacaccc acaaggcgcg cgtcgtgctg atcgaggccg
     6241 ggccgcgcgt gctggccggc tttgccgacg atctctccgc ctatgcgcag cgctcgctgg
     6301 aaagtatcgg cgtcgaggtg aagctcggac aggccgtcac cgaatgcacc gccgacagtg
     6361 tcgtttacgg cggcaaccgg ctggatacca aaaccatcat ctgggctgcc ggcgtgcgcg
     6421 cctcgcccgc cgccgaatgg ctcggcgcgc cggctgaccg cgccggccgc ctgcaggttc
     6481 tgcccgacct cacggtgccc aaccatcccg acatcttcgc catcggcgat accgttgtta
     6541 tcaacggccc cgatggcaag ccggtccccg gcattgcgcc ggccgccaaa caacagggac
     6601 actatgtcgc ggatgccatc aaggcgcgcc tgagcggcgg cacgcccgct ctgttccgat
     6661 accagcatgc cggcagcctg gcgcagatcg gcaagcgcaa ggcggtgatc gatttcgggc
     6721 gcatcaagct gcgcggcacc ctggcatggt ggatatgggg catcgcccac atctacttcc
     6781 tgatcggcct gcgcaaccgg ctcagcgtcg ccatcagctg gctgtgggtc tatgtgcgcg
     6841 accagcgcgc cgcgcggctg atcacgcaag gcagcagcaa ggtggtgcgg tgaggtttta
     6901 gctcggtggc tccatccttc gagacgcggc tgcgccgctc ctcaggatga ggctcctcat
     6961 ggtgaggagc gcggcaacgc cgcgcgtctc gaaccatgtg ccctgattcc ggcgtccatc
     7021 cgctcacttc accagcgaac agccgccgtc cgcgatcggg cggaacgcct gatcggcagg
     7081 gatggtcgaa accagccggt agtagtcgta aggatatttc gactcgtccg gcttcttgac
     7141 ctcgaacaga tacattggat ggatgacgcg gccgtcctgc cggatcgtga cctcgccgaa
     7201 cagtttgtcc ttgcccttga attttttcat ctgcgggacg acgtccttcg ccgcgtcgct
     7261 gccggtcgcc gccaccgcgt tgagataggc gagcgtggac gcatagacgc cggcctgatt
     7321 gccgctcggc atcttgccgt tgatgccggg ccgcgcggcg aaacgttttg caaaggcgcg
     7381 ggtgtcgtca tccatgtccc aatagaaggc ttccagcaat tgcaatccct gcgcgacctt
     7441 caatcccatg ccgtggacgt cgttgacgaa cagcaggaac gcgaccatcg tttgtccgtt
     7501 ctgctggatc ccgaactccg ccgcctgctt gaccgcgttg atggtatcgc cgccggcatt
     7561 ggcaagcccg atcaccttgg cttttgaatt ctgcgcctgc aacaggaacg acgcgaaatc
     7621 cgaggtgccg agcggatgct tggctgagcc cagcacctta ccgccatgct tttcgatgta
     7681 gttggtcgct tcggcctcga tgccctggcc gagcgcgtaa ttcaccgtga cgaaatacca
     7741 gtccttgccg ccgcgagcca tcatcgccgc cgccgtggtg ttgccggtcg cccaggcgtc
     7801 gttgacccac tggatggtgt tcggcgagca ggccttgccg gtgagatccg agcttgcggt
     7861 cgacgatgcc aggaacgtca tcctggaatc gcgaaccagt gaattgatgg tgagaccgac
     7921 agccgaattc ggcacgtcga caatggcatc gacgccatcg acatcgagcc atttgcgcgc
     7981 gatcgcagat ccgacatcgg ccttgttctg gtggtcggca tagacgatct cgaccttgat
     8041 attcttgccc ccgccgttga aatcctccgc cgccatcctt gcggcttcca ccgaacccat
     8101 gccgttggtg tcctggaaga tgccggaaat atcgttgagc acgccgaccc gcaccacatc
     8161 gccggaaatt tcggcacgcg ccgatcccca tgcggcgcag gacaacgcca gcgccaaata
     8221 aagcctgaaa tccctcatcg attctctccc tctggatttt gtctgttgcc attcccggac
     8281 gtggcgggca tccgttcagt ccagggcgtg ccgatcgtcg ccccgtcaaa gctgccgctc
     8341 cggcaggcgc aggacgagac catcaagctc ttgttcgatt ttgatctggc aggacaaacg
     8401 actgttcggc tgacgctcgg cggccacgcc atccagcaac gcgtcttctt catcactcat
     8461 ggcggcgagg cggccgatcc agttgtcgtc gacatacaca tggcaagtgg cgcacatggc
     8521 atttccgccg cactccgcca cgatctcgtc aacaccgtgt cgtgtcgcgg ccagcatggc
     8581 gctgtcgccg atgcgtgctt cgatgctctc ggaccggttg tcggagcgga tgaaagtaat
     8641 gatcggcatg ggattcagac tggcctgagg gtgatgggga gagtctccaa accgcgcagc
     8701 gtgttgttgt agcggcgctt cactggaccg gtgatctcga tggcggcgac ctttcgcgcc
     8761 agtgccgcca gcatcacctc gccttccagg cgcgcgacca gctggccgac gcacatgtga
     8821 atgcccgaac cgaacccgac atggccgctg gtcttgcgcg tgatgtcgta gcgatccgga
     8881 tcggcccatc gcctgggatc gcgattggcc gcgcccagga acatcaggat tttttcgccc
     8941 gcgccgacct tgcagccgct gatctcgaca tccttcgtcg tggtgcggaa gaaggtctgc
     9001 accgggcttt cgaagcggat cgcttcctcg aacgcattgc gcgccagaga tggatcgctg
     9061 cgcagccgcg caaattcttc cgggaagcgc gcgaggcaat agatcgcggc accaataccg
     9121 ttgacggtgg tatcgatccc cgccgagagc aacgagcgta ccagcatcgg cgcctccgcc
     9181 gcggcgatat cgccggcatc gacgcgggca tggatgcagg cgccgaaccc tccgggcgcg
     9241 agattgtccc gttggcattg ctcctgcaca taggcctgat gcggttgcga gcgttcgatc
     9301 gcggtctggc gcaattggtt cggtggcccg aacgcgttaa agaccacgcc ggcatagggc
     9361 agcagatgct cgcggccctc ctgtttcaac ccgacggcat cgggaaaaac cgacagcgga
     9421 taggcctcgg ccagatcggt gacggcatcg aagcttcctt tggccagcaa ctcgtcgacc
     9481 ttggcttcgg cggcagcggc gaacccgtca cggatctgct tcatgaccgc aggcgaaagc
     9541 acccggctga gcaccgcgcg cgtcctcgta tgagcgggcg ggtcagcctc gaggataaga
     9601 ctcggcggcc gccacggttt ctccttggca aaatcgctca aacccacgcc gcggctggaa
     9661 caaaacgtta cgggatcgtt gagcacgcga tggacttcgg cataacgcgc caccgcatac
     9721 acgttccatt tgtcgagatg caccagcggg cccgcctcgc gcaacgcctc gtgggtggga
     9781 aacggattct cgaagaactc gatcgcaaac ggatcgacat cgaggcccgg aatccccgaa
     9841 ggcacgccgc cggacgcagc acctgcctgt ccgccgccaa ccgcggtcga acctgttgtg
     9901 accatgtgag cctcccgaag tttagtcttg taaagctgcg gcttgtgtcc ctatttctcg
     9961 atggcgcgtt gggggatgat gcgaaacatg agcaagaacg gatcgaccgg ctcgcgccga
    10021 aaggcggcac tggcaagcgg cgtcaagccc ggccagacgc tcgatctcga gcgctacgtg
    10081 cccgccttcg tgaccttcat cgccaacaag ctgtcgcgca gcgccacggc gctttaccag
    10141 aagcgcttcg gtgtcaacgt caccgagtgg cggatcatgt cgctgctggc gatcgagccg
    10201 ggcatcaccg catcgcgcat ttgttacgtc atcggtttcg acaagggccc ggtcagccgc
    10261 acgctggcgc tgatgcaaaa acggggattg atcgcgatca agaccgaccc gcgcgacggc
    10321 cgcagccact cgatctcgct gacgccgaaa ggaagaaagg tccatgacca agttatcgtc
    10381 gcagccctcg agcgcgagcg ccggctgctc gcctgcctgc gaaaggacga gcgggaagtc
    10441 ctgatcgacc tgctgcgacg cgtccacagc aacctcgatg ccgtcacgga tcacggtgag
    10501 ccgggcgagg attgatctag tcgaagtcgg acccggcatt tccgagcaca ggccgttgaa
    10561 ccagccggcg ccggcgacca cgcgttcatt caaccccgac ccgccggtgc gctcaggcat
    10621 tcgcccactc ccctcacggc ctggcagttg aactgctctg tcgcgacgcc gtcgttagct
    10681 ggaagttccc acaaactagt tgccttggca acaataaaga tacggcggtt cccgcgggcc
    10741 aatcggcggc cttggcgcag gcaagcggaa gcgacgccgt cctttcgggc ggctaggccc
    10801 cggccatgac ggtgaaaaaa tacgcgtggc tcagcccgga cacccgatcc aagcggtcga
    10861 cgcatcgtct tccagaatcg ccacggcgct cgcgcgccgc gtcagcaccg cgcgctggtt
    10921 gatatagccc ttgtcggtga tttcgccgcc gtcgaccgag gccggctcgg ccagcagcag
    10981 cgcccgcgcc gcatgaccgg aggaaccacc gccttgcgcc ctgagttttg ccagccctga
    11041 cgcgattgcg gcgcgcactt tttcgttgcc gatcacctca ctcacatccg aactctcggg
    11101 caggccggca tgcacgcggc aggcactgag gttcggaaat accagaaaac gtacgtgatc
    11161 gccgccatgg ccggatacga cgatgtcctg cgccaaaggc gccagcgcgg tgatcccggc
    11221 gacgcgcagt gtgccgacgc tgacccaggt gccggaattg agtttgaaat cctcggcgac
    11281 acggccgtcg aaaaacaatc caagctcggg gcgctccgga tcggcgaacg tcacggcgtc
    11341 gccgatcaga tagaatccat ccgcgtcgaa cgcttgtgcg gtcagttccg gcgccttcca
    11401 gtagcccggc gtgacattgg gtccgcgcac ccgcacctcg agcttgtcgc ctgacggcac
    11461 cagcttca
//
LOCUS       DATNOI010000389         7029 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_962536,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000389 DATNOI010000000
VERSION     DATNOI010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962536"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VN968_15455"
     CDS             complement(<1..330)
                     /locus_tag="VN968_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30669.1"
                     /translation="MMKIPDPRRGLVRSFVNSLAFAAAAMLMLSAATDQRAEALSLIS
                     PGTTPAVTHASGGLTTEVRGGGHGGGGHGGGHGGFHGGGGGFHGGGAAFHGGGFHGGG
                     AMFHGGGF"
     gene            complement(501..803)
                     /locus_tag="VN968_15460"
     CDS             complement(501..803)
                     /locus_tag="VN968_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30670.1"
                     /translation="MKRWIGAAIMAAGLVLSGPAATGSAAAANSRVGLHAAGPHATDT
                     SKATDLSARRRVRHYRHDAYRSYDRPRYYDRPDDYRPYPYAAPVPFFLGFGFGPSW"
     gene            complement(1030..1317)
                     /locus_tag="VN968_15465"
     CDS             complement(1030..1317)
                     /locus_tag="VN968_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30671.1"
                     /translation="MNVMRVLGLAAVAALLMLIAPAERAQALSLASPGAAAAVQDDTR
                     EMTTEVYWRHHHHHWGYHRHWHHRHWHHRHWHHRHWRHHHWHHRHWHHRYW"
     gene            1562..2617
                     /locus_tag="VN968_15470"
     CDS             1562..2617
                     /locus_tag="VN968_15470"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HXL30672.1"
                     /translation="MVEVAAVGAVPAAAAPVNPGERIDAIDVLRGIALFGVLAINVVN
                     EFRVSIFEEFLPAASTTGTLDRAVQTFLTMAVELKAMALFSLLFGIGMAIQFERLAPD
                     PRRGMLLVRRLAILLVIGLAHLFLIWNGDILTEYSLAGFIVLPLLWGPRWLLAAGASL
                     FLALYLVMPLLPPVVPWPTTAWIKDHVAEARHVYGTGGFPEILAFRIRETASILPLHV
                     FILPRTIALFLFGMLVWRPDVVRRPAAHRLLLIAVAIIGIVVGAALTVASEQPALLGL
                     PLPGRIGFSLERLATLLLAIGYAAAIIGFVSTRPGMTVLAWVAPAGTHGFYELPGAIR
                     HLRLDFLRLWSRPVRPH"
     gene            2532..2795
                     /locus_tag="VN968_15475"
     CDS             2532..2795
                     /locus_tag="VN968_15475"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HXL30673.1"
                     /translation="MAFTNYLAQSVIFGWIFYGYGLGLFGRIDVVTALAIGVCVYVAQ
                     VMISAQWLRYYRFGPIEWLWRSLMYGTAQRLRIGRATPSGASA"
     gene            complement(3403..3639)
                     /locus_tag="VN968_15480"
     CDS             complement(3403..3639)
                     /locus_tag="VN968_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30674.1"
                     /translation="MRATQASRPMVTEASAQRRVRRPRLRVYPSYQVEPDDVYPRYFP
                     GRNAVRDCNATYVQEYRPSGTVIVPHVNCYWRRG"
     gene            3864..4262
                     /locus_tag="VN968_15485"
     CDS             3864..4262
                     /locus_tag="VN968_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HXL30675.1"
                     /translation="MWTKAKAPSLAEVEAMALEIFERLPKGFRDLCEGLIIRVDDFPT
                     DEVLDEMDAANEFDLLGLFQGIGLPFRSHNDVARLPNMIWLYRRPILDYWAEHDETLG
                     HIVRHVLIHEIGHHFGLSDGDMEAIEAAAG"
     gene            4357..4962
                     /gene="leuD"
                     /locus_tag="VN968_15490"
     CDS             4357..4962
                     /gene="leuD"
                     /locus_tag="VN968_15490"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HXL30676.1"
                     /translation="MEKFTTLEGVAAPLKIINVDTDMVIPKQYLKTIKRTGLGKGLFA
                     EQRYKDDGSENPDFILNKPAYRNAKILVAGDNFGCGSSREHAPWALLDFGIRCVISTS
                     FGDIFYNNCFKNGVLPIRVTQDDLDKLFDDAERGANATLSVDLAKQEIRGPDGGTVKF
                     EIDPFRKHCLLNGLDDIGLTMEKKASIDAYEAKATTARAWA"
     gene            4993..5781
                     /locus_tag="VN968_15495"
     CDS             4993..5781
                     /locus_tag="VN968_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30677.1"
                     /translation="MRPDVVTFWHGPLDRLRQTCLRSQVAAGHNVTVYSFEPLAGLPD
                     GVGNAEAEAVLSHAFAERLRPTGPDGVWRDWTTLQFSDFFRMRLMAQRAGLWLDADVL
                     LLRPVEIDLAKPYFAWERPRQLGNSVLYLPSGDPIVAAFAALMRQDELTPDWLALRHR
                     LTFALRRLRGSHRLSDVRVAIYGPAALTALARRAGELPNALPKKSFYAVHAEPKLFFE
                     PSDFSAVIGDPGIIGLHISPKGRGNQPPVPGSLYAWAAERFGTA"
     gene            complement(5820..6698)
                     /locus_tag="VN968_15500"
     CDS             complement(5820..6698)
                     /locus_tag="VN968_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HXL30678.1"
                     /translation="MIRPRRSLLFMPGSNARALEKARNLPVDGIILDLEDSVAPEAKA
                     MARDQIAQAIAQGGFGRREVLIRVNALDSPWWIDDVAMAGKARPDGILVPKISSVKDL
                     TAIADRLGDINADSSVRVWAMIETALAVLHAEELAASSCDSELRLAGFVFGPNDISRE
                     TRIRMQPGRAAMIPMITHCILATRAHGLEILDGPYSDFSNVDGFGQECAQARDLGFDG
                     KTLIHPGQIEACNAIFTPPAEEVAHARKIIAAFERPENASRGAIQLDGQMVERLHAEM
                     ARRTIAIADAIAAMGH"
     gene            6899..>7029
                     /locus_tag="VN968_15505"
     CDS             6899..>7029
                     /locus_tag="VN968_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HXL30679.1"
                     /translation="MSSFPEHLLEGYRAFTSQRLPTEQSRYRELSELGQSPEVMVIG"
BASE COUNT         1273 a         2241 c         2134 g         1381 t
ORIGIN      
        1 aaagccgccg ccatggaaca tggcgccacc gccgtggaaa ccgccgccgt gaaacgccgc
       61 gcctcctcca tggaagccgc ccccaccgcc gtgaaatcca ccatgcccgc caccatgccc
      121 cccaccgcca tggccgccgc cgcggacttc ggtcgtcagc ccgccggagg cgtgcgtcac
      181 cgccggcgtc gtgcccggac tgatcagcga cagcgcttcc gcgcgctggt cggttgcggc
      241 cgacagcatc agcatcgcgg ccgccgcaaa agccaaagaa ttaacgaagg agcggaccag
      301 cccgcgcctc ggatctggaa ttttcatcat gcgtgaaacc tcccgggatg ggcgacgaaa
      361 atcctggcca tgcagcggcc cgtcttcgac gcattcattt gcgcttcgtc ccggcattaa
      421 attagcgtta acgatgtgaa cgcgccgtga atgcacgcgc tgccggcgag aggatttgga
      481 gcgtagcaag cggcccgctg ctaccacgaa ggcccaaacc cgaagccgag gaagaacggc
      541 accggcgcgg cgtagggata aggccgatag tcatcaggtc ggtcgtagta gcgaggccgg
      601 tcataggaac ggtaggcatc gtgccgatag tgtcggacgc gacgccgcgc gctgaggtcg
      661 gttgccttcg atgtgtcagt tgcgtgcggg cctgccgcgt gcaagccaac tctcgaattg
      721 gccgcggcgg cgctcccggt cgcagccggc ccggagagca ccagccccgc cgccataatg
      781 gccgcaccaa tccatcgctt catggcagcg ttctcatgca ggatcgatca ccccataacg
      841 acaggcccgc gccgcgggcc tgtctcaatc tcactcacgc acaccagagc gttttcaagc
      901 gaagcatgcc ctcggacttg atccgggggt ggaaaccggt tcgcgtcagg aaaacgcgtc
      961 aaaatcaaga agccagagcc ccgtttcgat tctatcgaaa cgtaaaagag tttggcgtca
     1021 cgcctgatat taccagtaac gatggtgcca atggcgatga tgccagtggt gatgacgcca
     1081 atgccggtga tgccaatgcc ggtgatgcca gtgtcggtga tgccagtgtc ggtgatagcc
     1141 ccaatgatga tggtggtggc gccagtacac ttcggtggtc atctccctgg tgtcatcctg
     1201 aaccgcggcg gcggcgccgg gactggccag cgacagggcc tgggcgcgtt cggcgggagc
     1261 gatcagcatg agcaatgcgg cgacggcagc aagccccaga actctcatca cgttcatatg
     1321 tttcctccgt ttgtcatcgg acaacacgcc agcgtcgtgc ggctgatctg ttcgaccgcc
     1381 tcaccgcaac gcagatcgca aggctagagt cactgacgtg aatgtggaat gaataaccgc
     1441 gccgcatgga tgaatgcgaa tggtgcgaac cggttcccgc tgcgcataac aatcttgtcg
     1501 aggtgctcaa aatagcgcag ttgggcggct ctctatcgaa caactgtctt gaaagggctc
     1561 catggtcgag gtcgctgcgg ttggagcggt tccggcggcc gcggctcccg tcaatcccgg
     1621 tgaacgcatc gacgccatcg atgtcctccg aggcatcgcc ttgttcggcg tgctcgcgat
     1681 caatgtcgtg aacgagtttc gcgtttccat ctttgaagag ttcttgccgg ccgcaagcac
     1741 cacggggacg cttgaccgcg ccgtccaaac cttcctgacg atggccgttg agttgaaggc
     1801 catggccctg ttctcgctgc tgttcgggat cggcatggcc atccagttcg agcggctggc
     1861 gccagatccg cgccgcggca tgctgctggt gcgcagactg gcgattctgc tggtcatcgg
     1921 cctggcgcat ctgtttctga tatggaacgg cgacatcctc accgaataca gtctggcggg
     1981 attcattgtg ctgccattgc tgtgggggcc gcgctggctg ctggcggccg gcgcgtcact
     2041 atttctggcg ctttatctgg tcatgccgct gttgcctccc gtcgtgccgt ggcccaccac
     2101 ggcatggatc aaggatcatg tcgccgaagc gcggcacgtc tatggcaccg gtggatttcc
     2161 ggagatcctc gcgttccgta tccgggagac ggcatccatc cttccactcc atgttttcat
     2221 tcttccgcgc accatcgcgc tattcctgtt tggcatgttg gtgtggcgcc cggacgtcgt
     2281 tcgtcgtcca gcggcgcacc ggcttctgct catcgccgtc gcgatcatcg gcattgtcgt
     2341 cggagccgcg ctgaccgtgg ccagcgaaca gcctgccctg ctggggttgc cgttgcccgg
     2401 gcgaatcggt ttttccctcg aacggcttgc cacgctgctc ctggcgatcg gctatgccgc
     2461 cgccatcatt ggtttcgtga gcacccgacc gggaatgacg gtgctggcat gggtcgcccc
     2521 cgctgggacg catggctttt acgaactacc tggcgcaatc cgtcatcttc ggctggattt
     2581 tctacggcta tggtctcggc ctgttcggcc gcattgacgt cgtcaccgca ctcgcgatcg
     2641 gtgtttgcgt gtatgtcgcg caagtgatga tcagcgccca atggctgcgg tattatcggt
     2701 tcggccccat cgagtggctg tggcgcagcc tgatgtacgg cacggcgcag cgtttacgca
     2761 tcggccgagc caccccttca ggcgcgtccg cgtagctgac gacaggaggc catcccctcc
     2821 cttttccggc gccacggagt tccgctgtta tcgccgagcg gattggcatg acaacattct
     2881 taaaaccggc ataggctgtc gcccgtatct tctgaacgct tctcgtcaca ggagattgtc
     2941 atggcatcgt ctcgaaaaat gccctggata tcgcgctttg ctttcgcacc tgctggtatc
     3001 gccttgtttt cagcgggcca agccctcgca tcgcagggac ccggcggagg tccgggcacc
     3061 gcgagcagct tcacgcagct cgccatggcg attatcgtct atggcacgtc ggcgctggtg
     3121 gtctgtgccg gactgatcgg tgcggcgcgt cggcgtcgag cgcgggcaat atcgccaagc
     3181 gttttcaagc gaaggcccgt ccccgacttg acccggggtg gccgccggtt cgtcgaaaga
     3241 gaacgcgtca aaagaaagac acggcctcga ttctgatgca atcaggaccg atcaggcccc
     3301 aaaagcgttt tcgagcgaag tggaaaccgg ttcgcgtcaa gaaaacgcgt caaatcaaga
     3361 atcttgagcc ccgtttcgat tccatcgaaa cggataaagg ctctagccgc gccgccaata
     3421 gcagttgacg tgcggcacaa tcaccgtgcc gctcggccgg tattcttgca cgtaagttgc
     3481 gttgcaatct cgcaccgcgt tgcggccggg aaaataccgg gggtagacgt cgtcgggttc
     3541 gacttggtag ctcggataaa cgcgcaagcg tggccgccgg acccggcgct gcgccgacgc
     3601 ctcggtgacc atcggccgcg acgcctgcgt cgcccgcatc cccgatggcg ccgtttgggc
     3661 ctcgactccg gccgtgggca gcgaaaaact gcctgcaaaa accagcaata ttgccgccgt
     3721 ccggatccgc atcatctcgc ccacccaaag ctgttcaacg gccgcaaggt ctccgattgc
     3781 cgcgccaacg tcaacagcgc gtgaagtcca gaatccaagt cggggatgca cttgtgcgta
     3841 acacgcggta agaagccgtg atgatgtgga ccaaagccaa agcgccctcg cttgccgaag
     3901 tggaagccat ggcgctcgag atcttcgagc gcctgccgaa gggctttcgc gacctttgcg
     3961 agggcttgat catccgcgtc gacgattttc cgaccgacga agtcctcgat gaaatggacg
     4021 cggcaaacga attcgatctg ctcggcctgt ttcagggcat aggcctgccg ttccgcagcc
     4081 ataacgacgt cgcccgcctg cccaacatga tctggctcta ccgccggccg atcctggatt
     4141 actgggccga acatgacgag acgctaggcc atatcgtccg ccacgtgctg attcacgaga
     4201 tcggccacca tttcggcctg tccgacggcg acatggaagc aatcgaggcg gcagcaggtt
     4261 aggattgcgc aattgggcct ttgattgagc tcctattcgc gataaaccac cgcccaacgg
     4321 cctatccttc aattttttgc cgggaagtgc gatcaaatgg aaaaattcac cacgctggaa
     4381 ggcgtcgcgg cgcccttgaa gatcatcaac gtcgataccg acatggtgat tcccaagcaa
     4441 tatctgaaaa ccatcaagcg caccggcctt ggcaaggggc tgttcgcgga acagcgctac
     4501 aaggacgacg gcagcgagaa cccggatttc atcctcaaca agccggccta tcgcaacgcc
     4561 aaaatcctgg tcgcgggcga caatttcggt tgcggctcga gccgcgaaca cgcgccctgg
     4621 gcgctgctcg atttcggcat ccgctgcgtg atctcgacct cgttcggcga catcttctac
     4681 aataattgtt tcaagaacgg cgttctgccg atccgcgtca cccaggacga cctcgacaaa
     4741 ctgttcgacg atgccgagcg cggcgccaat gcgacgctga gcgtcgatct cgccaagcag
     4801 gagatccgcg gccccgacgg cggcacggtg aagttcgaga tcgatccgtt ccgcaagcac
     4861 tgcctgttga acgggctcga cgacatcggc ctcaccatgg agaagaaggc ctcgatcgac
     4921 gcctatgagg caaaggcgac gaccgcgcgc gcctgggcct gatcccaacg tccaccaggc
     4981 gaggcgacgt ggatgcggcc cgacgtcgtc accttctggc atggcccgct cgaccggctc
     5041 cggcagacct gcctgagatc gcaggtcgcg gcgggtcata acgtcaccgt ctacagtttc
     5101 gagccgctcg cgggactgcc cgacggcgtc ggcaacgccg aggccgaagc ggtgctgtcg
     5161 catgcttttg cggaaaggct gcgcccgacc ggccccgacg gggtatggcg cgactggacc
     5221 acgttgcagt tcagcgattt tttccggatg cggctgatgg cgcagcgcgc gggcctctgg
     5281 ctcgacgccg atgtgctgct gctaaggccg gtcgagatcg acctggcaaa accctatttt
     5341 gcctgggagc ggccgcgtca gctcggcaat tcggtgctgt atctgccatc cggcgatccc
     5401 atcgtggcgg cgttcgccgc gctgatgcga caggacgaat tgacgccgga ctggctggcg
     5461 ctgcggcacc ggctgacttt cgcccttcgc aggctgcgcg gttcgcatcg gctctcggat
     5521 gttcgtgtcg cgatctatgg tccggcggcg ctgaccgcgc tcgcccgccg cgccggcgag
     5581 ttgcccaacg ccctgccgaa gaaatcattt tatgcggttc acgccgaacc gaaactgttc
     5641 ttcgagccct cggatttttc agccgtcatc ggcgatcccg gcattatcgg actgcacatc
     5701 tccccgaaag gccgcggcaa ccagccgcct gtgcccggca gcctgtacgc ctgggccgcg
     5761 gagcggtttg gtacggccta aggcgtgagg tctgttcccc tctccccttg tgggagagat
     5821 cagtgcccca tcgccgcgat cgcatccgcg atggcgatgg tgcgtctggc catctcggcg
     5881 tgcagccgtt ccaccatctg gccgtcgagc tggatcgcgc cgcgcgaagc gttttccggg
     5941 cgttcgaatg ccgcgatgat cttgcgggca tgcgcgactt cctccgccgg gggcgtgaag
     6001 atcgcgttgc aggcctcgat ctggcccgga tggatcagcg tcttgccgtc gaagccgaga
     6061 tcgcgcgcct gcgcacattc ttgtccgaag ccgtcgacat tggaaaaatc gctgtagggt
     6121 ccgtcgagaa tttcaaggcc gtgcgcgcgc gtcgccagaa tgcagtgcgt gatcatcgga
     6181 atcatcgccg cgcggcccgg ctgcatccgg atccgggtct cccgcgaaat atcgttcggt
     6241 ccgaacacga agccggcgag ccgcagttcg gaatcgcacg aggatgccgc caattcctcg
     6301 gcatgcagca ccgcgagcgc ggtctcgatc atcgcccaga cccggaccga actgtccgcg
     6361 ttgatatcgc cgaggcgatc ggcgatggcg gtgagatcct tcacgctgga aatctttgga
     6421 accagaatgc cgtcgggcct ggccttgccg gccatggcga cgtcatcgat ccaccagggc
     6481 gaatcgagcg cgttgacgcg gatcagcacc tcgcgcctgc cgaacccgcc ttgggcgatc
     6541 gcctgcgcga tctggtcgcg cgccattgcc ttggcctccg gcgccacgga atcctcgagg
     6601 tcgaggatga tgccgtcgac ggggagattg cgcgcctttt ccagcgcccg cgcattcgat
     6661 ccgggcatga acaagaggct gcggcgcgga cggatcatgg ccttgttccc ttcgattttt
     6721 ctatcccacg cgataaagcc ggttcgattt cgtcgaaatc ggattgttcg acagcttatt
     6781 gtttgagcat gatcttatcg gaaaaccggg tgcccacttt ttcggatcat gctctaacat
     6841 ttcaaaccct cgcccgaaag gcttgttcct gccgactgca tatggtttag cccccgccat
     6901 gtcatccttt ccagaacatc tgctcgaagg ctatcgcgcc ttcacctcgc agcggctgcc
     6961 gaccgaacag tcgcgctacc gcgagctgtc cgagctcggc cagtcgcccg aggtgatggt
     7021 gatcggctg
//
LOCUS       DATNOI010000390        18547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1615539,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000390 DATNOI010000000
VERSION     DATNOI010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18547
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615539"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(404..1204)
                     /locus_tag="VN968_15510"
     CDS             complement(404..1204)
                     /locus_tag="VN968_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL30680.1"
                     /translation="MERREVLKTMAIAGLAASAGGATANAQTTAQTFVLVHGAWHGGW
                     CWSRVADRLRTAGHQVFTPTQTGLGERKHLLSKDITLDTFIKDIVNVIEAEELSNVIL
                     VGHSFGGLAISGTADAMPDRIRHLVYLDSLMVEGGKSPFDSLPPDIVAARRKAAEETS
                     GGLSLPAPPPSAFGVSDAKDTDWVKRRLTPHPLGTYTSTLNIKGPVGNNLPRTYIHCT
                     TPFYAALQGSRNWVKAQQGWRWAEIATGHDAMMMAPEELTRMLIGVTS"
     gene            1698..2240
                     /locus_tag="VN968_15515"
                     /pseudo
     CDS             1698..2240
                     /locus_tag="VN968_15515"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
     gene            2331..3494
                     /locus_tag="VN968_15520"
                     /pseudo
     CDS             2331..3494
                     /locus_tag="VN968_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137555.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3537..4103
                     /locus_tag="VN968_15525"
     CDS             3537..4103
                     /locus_tag="VN968_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL30681.1"
                     /translation="MIAPLKPGVTLARPAGPVIETERLKLRQWCGADVAPNTAMLSDP
                     GTARFITVDGKPVIDELVGWRNAAVISGHWALHGAGMFVVEERASGKFVGRVGPWFPP
                     GWPGFEVGWGIARDFRGKGYAVEAARAAIDWSFATFELDSIVHCIDRENTASQAVARR
                     LGAKKQGETDLFGHLSDVWVTTRGSWLA"
     gene            4281..5357
                     /locus_tag="VN968_15530"
     CDS             4281..5357
                     /locus_tag="VN968_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL30682.1"
                     /translation="MLISTLDLALRAGTTALLLILAASLFRDFRGSVAGRLAVAFALG
                     SAAHAVTYGIGATSPVSIWHAPLIALSTGNIVVFWLFTHALFDDAFKMRWWHALVWAA
                     VAGFSFINCAWIAPASGNVRFAILAINVLVLGFIALAVGQTIASWSADLVEGRRRLRV
                     FIVSAAALYGGINAVLQILVSGREAADVASAANAALLAGIVAAICYSMMWVAAADLFP
                     VAPVVAAVASVPAPSVAAPDSGAADQRLVDALMRLMADERIYRHDNITIGTLATKLRI
                     PEYRLRRLINQRLGYRNFNVFLNNHRIEEAKAALADPSQAEVPVITIAMDAGFQSLGP
                     FNRAFKATTGVTPTEYRRLNEARA"
     gene            5509..6919
                     /locus_tag="VN968_15535"
                     /pseudo
     CDS             5509..6919
                     /locus_tag="VN968_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136058.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
     gene            7024..7902
                     /locus_tag="VN968_15540"
     CDS             7024..7902
                     /locus_tag="VN968_15540"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXL30683.1"
                     /translation="MARPDTKNTTKYWWDRHISGLSLMHADFTCHDFAPHTHDAFVIA
                     VTELGGARIKSRGVTDVARSSRLFVSNPEEPQSSWMGDSPRWRYRSMYLTQAAIDVVA
                     EGLGIESVPFFTGNMIGDADLVSGFASLHQALEVGADRFREHELLISAFGHLFGRRGS
                     GGNRIEPAPRDSILVRRIADLVRERFAENLLLGDLAAAAGLSIFQLIGLFKRSIGTTP
                     HVYLTHVRLNAACRRLRLGDSIADAAAAAGFCDQSALTKHLKRCYGITPRQFAKAAAS
                     ARLLRRAPPGAARNPG"
     gene            7978..8901
                     /locus_tag="VN968_15545"
     CDS             7978..8901
                     /locus_tag="VN968_15545"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXL30684.1"
                     /translation="MIPPSSRKARSTVAVIGLGSIGGVVAGCLRAADRHDVIAGVRQP
                     IERLVLERPEGNVEVALRAFTDPAQAGPVDWVLLCTKTQQTPLTAPWLARFCDSSTRV
                     AVLQNGIGHLAHVAPLVGDAGIVPALVYYNGERLGADRVRLRHVAEHDLAVADDANGR
                     AFAELLDGTPLRVLLSSDFPTLAWRKLLINAVANPITALTLQRQAVFRRDDIKALCVA
                     ILDEAVAVARADGAQMAEDESLRTISALLTYPPEAGTSMYFDRLAGRALEVEALTGAV
                     VAAGERLRIATPLNGALLALLRAVSDAARTG"
     gene            complement(8909..10279)
                     /locus_tag="VN968_15550"
     CDS             complement(8909..10279)
                     /locus_tag="VN968_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXL30685.1"
                     /translation="MAEETATLAAYVADLKFADIPHEVLDRAKVLTLDFLGSAIRARR
                     DAESTPSLLKMLEALALDGKGQSTVFGDAKTWTPAVAALLNGALGHSLDFDDTHADSS
                     LHPSAPVVPAAFAVGELVGASGRDVLTAIVAGYEVCCRLGNALDPTSHYARGFHPTAT
                     AGTYGAAAAAAKLFGLSKQQIIAAFGVSGSQAAGSLQFLVNGAWNKRYQVGAAAMNGV
                     IAATLARNDFVGATESVEGQHGLLVGYSDNAHPDKVVAGLGEMYETMKIGVKPYPSCR
                     YTHAALDALIAMRREHNLTPDQIKRVEIGLHRNGITLTGDAATKRHPTSIVGGQFSMF
                     FTGAVALDQGRFGWDDYQRLGDTAVDALADKFDVVQDDRLEIGRSHPFGARVSITTDD
                     GVHERLHADPSGEPNSFPDAQAMAQKFLTLARPVLNGRADQFADAILSLELFDRVEKA
                     TQLGRQ"
     gene            10415..11509
                     /locus_tag="VN968_15555"
     CDS             10415..11509
                     /locus_tag="VN968_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HXL30686.1"
                     /translation="MIAFLKRLSRLLRSRRAQLALAFRVTVAAAGAYAIATAIHLRLP
                     LWAVLTSLIVTQMSVGRSLKATRDYMFGTVGGAIYGGAIAVLIPHSGEVELLGLLVLA
                     VAPLAFIAANNPSLNAATVTAVIVLLVPIMSHVDPLASAIDRVMEVTVGALTGLLVTF
                     FVLPSRAHNQIRITATRTLELIAVAFSKLLDGLTHGLDNEALHRIQDGIGAALVGLNA
                     TGAEAERERAAHLSRGPDTGPLLRTSLRLRHDLVMIGRACMMSLPSDLQARLAVPLDK
                     VSDAVVTYLRSAAASLRTRGNSPSSSPIDEALQLYAAEVAALRSEGLIRVLPGDAAER
                     FFALGFALEQMRQNLNDLGRCVAEWSETPP"
     gene            complement(11526..12119)
                     /locus_tag="VN968_15560"
     CDS             complement(11526..12119)
                     /locus_tag="VN968_15560"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HXL30687.1"
                     /translation="MHGGYEAITALPAYAAYVERRKNQAKSDKGRRWKVKKPAGKQDV
                     DEAERQLGAELPKDYRKFLTTYGETELLVRLPGHSGELRFYKPLELATQRDNLFKFIT
                     RTEKNREKVSAYFRKEYGVSLRDLVPVAEPAQESRCVVIHLEKGDRFGWCFHWDHDGA
                     WELEHPSPNFDAALKALTDGIAQCDTAMLSFLGVYLD"
     gene            complement(12650..13378)
                     /locus_tag="VN968_15565"
     CDS             complement(12650..13378)
                     /locus_tag="VN968_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30688.1"
                     /translation="MIIGFRMRGSVEIQTEDFLRVPFSEIGNVVAAYRRYAAELVAAG
                     YMETTHTRHTRRNLRCPEAKLEWQKSLDDLMLAALSASLEDQARHLAALQDTPAAHEP
                     LYLWLAAHRSFAAADSHEHTIQLAEAARDTLALRRAEKTPHYAWSIAENDLEARIFDV
                     LSWAHLRAENPAAALDAIEQAYRIAPSNDRGVQRATILCDHFPDRRQEALAAANKRAE
                     LDGREEDHRAAGSCRICGVVQAQA"
     gene            complement(13605..14261)
                     /locus_tag="VN968_15570"
     CDS             complement(13605..14261)
                     /locus_tag="VN968_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HXL30689.1"
                     /translation="MTLSVDLFFSFRSPYSYLALPKTLRLAANYDVTVTLRPVYPLAV
                     RVPGFFKRTNPQFARYVVLDSSRVARRENIPFRFPRPDPIVQDMTTLDVAENQPYIHR
                     LTRLGAAAQREGRSLGFADAISRVLWDGTVSGWNEGDHLAHAAAAAGFDLAAMDAAIS
                     ADPDSYERIIADNEKDHAASGHWGVPTFVFENEPFFGQDRIDLLIWRMQDKGLTRRKS
                     "
     gene            complement(14348..14819)
                     /locus_tag="VN968_15575"
                     /pseudo
     CDS             complement(14348..14819)
                     /locus_tag="VN968_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
     gene            15585..15845
                     /locus_tag="VN968_15580"
     CDS             15585..15845
                     /locus_tag="VN968_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HXL30690.1"
                     /translation="MFSRPTAGFVRATLAEACLIGLLVLSLSRAGAQDGQGGARQACE
                     ADYHRLCAGISPGGGRIKKCLNDNFDALSDACKQVVGAGTGK"
     gene            complement(15913..17223)
                     /gene="murA"
                     /locus_tag="VN968_15585"
     CDS             complement(15913..17223)
                     /gene="murA"
                     /locus_tag="VN968_15585"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXL30691.1"
                     /translation="MTRVPLQYIVEGGHRLAGTIEPSGNKNAALPIIAAALLTEHPVT
                     LENVPRIRDTETLVELIRSVGASAEWRQRNTLAIHARNIRAADLDPVLCARIRASILL
                     AGPLLARCGEVMLPPPGGDVIGRRRLDTHFLAFEQLGASVVANDRLQFRCLRLKGADV
                     FLDEPSVTATENALMAAVAAYGTSHLRNVASEPHVQDLAHFLVALGARIEGIGTNTMV
                     VHGPATLGAATYSIGPDHIEVGSLIGLAAVTRSPLRIARAGIEHLRSIRMGFERLGIV
                     CEVDCDDLVVPSGQTLKIRDDFGGHVPKLEDQPWPAFPADLMSIAIVTATQCDGVILM
                     FEKMFESRMFFVDKLIAMGGRIVLCDPHRAIVAGPSRLKGAPMISPDIRAGMAMLLAA
                     VCAKGVSTIDNADQIERGYERIDERLNALGARITRVPERTQDAG"
     gene            complement(17220..>18547)
                     /locus_tag="VN968_15590"
     CDS             complement(17220..>18547)
                     /locus_tag="VN968_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HXL30692.1"
                     /translation="AAWFEEHPAEAKVIVGKVVEAAAAREAARKARELTRRKGALDIA
                     SLPGKLADCQERDPAKSELFIVEGDSAGGSAKQGRNREFQAVLPLRGKILNVERARFD
                     KMLGSEQIGTLITALGTGIGRDDFDLVKLRYHKIILMTDADVDGAHIRTLLLTFFFRQ
                     MPALIEGGYLYIAQPPLYKVTRGKSEQYLKDERALEDYLIATGLDDCVYKLASGEDRG
                     GRDLLALVEDARIIRGTLHNLHSRYNRKVIEQAAIAGVLSPKITGDAPTANAAADYIA
                     RRLDALADEVERGWTGRFTEGEGFFFERTVRGVKDVAIIDDALLGSADARKLDDYAAR
                     LQEAYPKPGLLRRKDTETPIHGPVSLFEAVTDAGRKGVALQRYKGLGEMNPGQLWETT
                     LDTNERSLLQVKIKEVDEADDIFTKLMGDIVEPRREFIQDNSLSANVDV"
BASE COUNT         3179 a         5775 c         6016 g         3577 t
ORIGIN      
        1 gcttgccgtg cggcagcgcg cggcgcacct cgaatccatc accgaggtcg cgggtgcgcg
       61 gcacgatgat cagctccagc gcgtcgcagg agtgcggatc gcccagcacg ggatcgttgg
      121 tgggaagcca gctcatgagg agatctccgt gattttgccg gcgatcatag cagcaattat
      181 ccggtgtcgt ccaagcacgg agtcatcaca atctccgggt cgtcccggcg aaatgccgga
      241 tcctctttct tcccctctcc ccttgtggaa gagggtgcct gagcgaagcg aaggcgggtg
      301 agggggtgat gagtctcgtt gtgtcgcgcc ccctcatccg gcacggactt cgtccgcgcc
      361 accttctcca acaaggggag aagggaagaa aacagcgaat cgctcaactg gtaacgccga
      421 tcagcatgcg cgtgagttct tccggcgcca tcatcatggc gtcgtggcca gtggcgattt
      481 cggcccagcg ccatccctgc tgcgccttga cccagttgcg cgagccctgc agcgccgcat
      541 agaacggcgt ggtgcagtgg atataggtgc gcggcaggtt gttgccgacc gggcccttga
      601 tgttgagcgt gctggtgtag gtcccgagcg gatgaggcgt cagccggcgc ttgacccagt
      661 cggtatcctt ggcatcggaa accccgaagg cggatggcgg cggcgcgggc aggctgaggc
      721 ctccgctggt ttcctcggcg gccttgcggc gcgcggcgac gatatccggc ggcaggctat
      781 cgaacgggct cttgccgcct tccaccatca atgaatccag atagaccaga tgacgaatcc
      841 ggtccggcat cgcgtcggcc gtaccgctga tggccaaccc gccgaacgaa tgcccgacaa
      901 ggatgacgtt ggacaattcc tcggcctcga tgacgttgac gatgtccttg atgaaggtat
      961 ccagcgtgat gtctttcgac agcagatgct tgcgctcgcc gaggccggtc tgcgtcggcg
     1021 tgaacacctg gtgcccggcc gtgcgcagcc ggtccgcgac ccggctccag caccagccgc
     1081 cgtgccaggc gccgtgaacc agcacgaatg tctgcgccgt ggtctgcgcg tttgcagttg
     1141 ctccgcccgc gcttgctgcc aatcccgcga tggccattgt tttcaggact tcccgacgtt
     1201 ccatgttggt tgctcccttt gtgaatttgc ggtcccggct cattgcgatc ggccggagat
     1261 ggatcaatgt atcagtgttt tgaccaattg gcaggacgcc cccggagcgt tcggccgccg
     1321 atccgcccgc aacgcgcagc ggaatcatcc agcacctcag cgccggtact acgatgctca
     1381 tcatccgaca gacagctcat cgcgcccgcg ccactctgca gcccacgcgc tgtcccacgc
     1441 gtggcacaac gcagcctggt gggcggtttc cacgcggggt ttcctgtgtt gcaaatttgc
     1501 acagggccgc ggttgagcta aggtgcaacc tcaggaggcg attcgaaagg tcgactgctc
     1561 tcgctaacgc tcgaaggtgt ttctattgcc cttggaaacg atttggctgg gtcgcagcga
     1621 tccaatctcc gcgccggagg aaattcgggt gctgatggct tcggttgccg aacacgcaat
     1681 ggacggttcg gaccacaatg cgaggagatt gccggcctca ctgcggcgcg ccggaccatg
     1741 cgccagtgac tgacgccgct gcaagaggtc agaagttgct gcgccagcag gcggccgtcg
     1801 cccgtttcgg tggcttcgcg ctccgcgagc cggatttgac gaaaatcctg accgaggcgg
     1861 cgcgggcctg cgccgatggc ctcggcgtgc ccttcagcca ggtctggcaa catcgcgccg
     1921 agacgaacga tcttgtcgtc gtcgccggcc atggctggcg ggatgatgtc gtcggccatg
     1981 ccgtgtcgag cgccgatatg agatctccgc aggcgcgcgc cttcacggct ggcgaaacct
     2041 cgatcgaggg tgatttgcaa aacaggaacg gttgcggact gcctccgttc tatgccgcgc
     2101 acggcatcgt ctcgatcgtc gacgtcatca tcaaggatga cggtgaccag ccttacggac
     2161 tacttgagat cggcgccgat cggccgcgcg attgcgaccg ggacgacatc catttcctca
     2221 ccggtttcgc cgacgtcctg gcggaggccg tttcgtctgc ctcccgggag gcggcgttcc
     2281 aggtcagcgt cgaccaatta aaggccctta tcgaggaaaa ggaccgcctg ctgtgcgaga
     2341 agaacgtcgc ggacctgcag ctccgccagg cccagaagat gcaggcggtt ggtcaattga
     2401 ccggtggcat cgcccacgat ctcaataaca ttctgacggt cattaccgga acgatcgaaa
     2461 tcctggcgga aggcgtcgcc gaccggccgg aactgctggc gatcaccagg atgatcgacc
     2521 aggccggggc acgcggcgcc aatctcaccc agcggctgct ggccttcgcc cgcaaacagc
     2581 cgctgcagcc gcgcgaggtc gacatcaatg cgctggtgat ggaagcagcc aacctgctgc
     2641 gtccgatgct gggcgagcag gtcgaggtcc acctgatgct cggtgccgat acgtcgcgcg
     2701 cgctgatcga tccgcgcccg ctcacaagcg ccatcctcaa tctcgcgctc aacgcccgcg
     2761 acgcgatggc gaacggcggc aagctcatca tcgagaccgg caacgtcgtc ctcgacgaca
     2821 gctatgccgc catccatagc gatgtcaccg ccggaaagta cgtaacggtc gcggtgattg
     2881 attccggcca cggcattccc gccagcattc tggacaacgt tttcgaaccc ttcttcacca
     2941 ccaaggacgt tggcaagggg tccggcctcg gcctcagcat ggtctatgga ttcgtcaaac
     3001 agtccaacgg gcatatcaga atcgaaagcg aggaaggccg cggcacgacg gtgaggatct
     3061 atctgccgca agcgatcggc gtcgccgagc ctgtcgagcc agcgggcacg tcaagcatcg
     3121 aaggcggccg tgagaccatc ctggtcgttg aggacgatca tctggtgcga accttcgtcg
     3181 tcggtcaaat tcaaagcctg gggtattcga cgctggcggc agtcaacgcg gcagaagcct
     3241 tggtcgttat cgatggtccc caggaaatcg atctgttgtt caccgacatg atcatgccgg
     3301 ggtcgatgaa tggccgtcaa ctcgccgacc aggcgctgca gcgaagggct tcgctgaaaa
     3361 tattgttcac gtcaggttat tccaacgaaa ccatcctcca ccacggccat ctcgatgccg
     3421 gtgggttgct gcttgccaag ccgtaccgca agtcggatct tgcacggatg atacgcgccg
     3481 cccttgtcgc ctgatgcaag gccgcgggta tttgtcctcg aagaacggaa gttgcgatga
     3541 ttgctcccct aaagccgggc gtcacgcttg cacgacctgc cggccccgtc atcgagaccg
     3601 agcgtctcaa actgcggcaa tggtgcggcg ccgacgtcgc accgaacacg gcaatgctgt
     3661 ccgatcccgg caccgcgcgg ttcatcaccg tcgatggaaa gccggtcatc gacgaactgg
     3721 tcgggtggcg gaacgcggcg gtgatatccg ggcactgggc gttgcatggc gcgggcatgt
     3781 tcgtggtcga ggaacgagcg agcggaaaat ttgtcggccg ggtcgggccg tggtttccgc
     3841 cgggctggcc cggctttgaa gtcggctggg gcatcgcccg cgattttcgc ggcaagggct
     3901 atgccgtcga ggcggcgcgg gcggcgatcg actggtcgtt cgcgaccttc gagctcgatt
     3961 cgatcgtcca ctgcatcgat cgcgaaaaca cagcatcaca ggcggtggcg cggcggctcg
     4021 gcgcgaaaaa gcaaggcgag acagacttgt tcggccatct ctcggatgtc tgggtgacaa
     4081 cgcgtggttc gtggctggct tagagcggga tgacttttct tcgaatcgtc atcccgctct
     4141 atctttttgt ttgagcatga tcttttcgga aaaccgggac ccacttttcc ggatcatgcc
     4201 aaagcgtgct gaaattgaac cgtcaggcgg acttcgctag actggccgtc atacccgcgg
     4261 cccgtgattg aggcgaccgg atgttaattt ccacactgga tctcgcgttg cgcgccggta
     4321 ccacggcctt gttgctgatc cttgccgcat cgctgtttcg cgacttccga gggtccgttg
     4381 ccggccggct ggcggtcgcc tttgcactgg ggtccgcggc tcacgcggta acctacggca
     4441 tcggcgccac gtcgccggtt tcgatctggc atgcgccgtt gatcgcgctg tctaccggca
     4501 acatcgtggt gttctggctg ttcacgcacg cgctgttcga cgacgcattc aagatgcgct
     4561 ggtggcatgc gctggtctgg gcggcggtgg ccggtttcag tttcatcaat tgtgcctgga
     4621 ttgcaccggc atcggggaac gtgcggtttg ccatcctcgc gatcaatgtg ctggttctcg
     4681 gcttcattgc cttggcggtc gggcagacca tcgcctcctg gtcggccgat ctggtcgagg
     4741 ggcgccgtcg cttgcgggtt tttatcgtca gcgccgccgc gctctacggc ggcatcaatg
     4801 ccgtcctgca gatcctggta tcgggccgcg aggcggccga tgtcgccagc gctgcgaatg
     4861 ccgccctgct ggcgggcatc gtcgccgcga tctgctattc gatgatgtgg gtcgcagcag
     4921 cggatctgtt tccagtggca cctgtcgtcg cggcggttgc gagcgtaccc gccccatccg
     4981 ttgcggcgcc ggattcgggc gccgcggatc aaaggctggt cgatgcatta atgcgattga
     5041 tggcggatga gcgaatctac cggcacgaca acatcaccat cggcacgctg gcgaccaaac
     5101 tcaggattcc cgaatacaga ttgcggcgct tgatcaacca gcggctgggt tatcgcaatt
     5161 tcaatgtctt cctcaacaat caccggatcg aggaggccaa ggccgctctg gccgacccct
     5221 cgcaggccga ggtgcccgtg atcaccatcg cgatggatgc aggctttcag tcgcttgggc
     5281 ccttcaatcg cgccttcaag gcgaccaccg gcgttacgcc gaccgaatac cgccgcctca
     5341 acgaggcccg cgcctagctt cctaacctat tggggtaggt ggcatattcc ggaatcggcg
     5401 agggctgtca gttttccgcc actgcgattt ccgaattcgg cgagccgtcg cgccgtaatc
     5461 gaggccatgt ccggcacgcc atgatggcgc caattgccgg agaaaaccgt gacccgaaag
     5521 catcaaatca tcctccttgt gacgacaaca ttatgcggcg ccgcggcgct gaccgcggtt
     5581 cgcgcgaacg acgcaccgcg ggtcgcgacc ggctttgtcg cgcatgtcct ctgttcggag
     5641 acctttgtat ccgggcttga tccggatcgg gtgttctcgg agacgatggc cgccatgccc
     5701 ggcaccgggc tgatcagatc gatcgtgcaa ggaaggacgt gaccgtgacg ctgctcggtg
     5761 gcggacaaag ccatgccgtc ttccgcgaag gtctgggctg ttacgtctct catgacgatg
     5821 cctccgtcga tggcttgccg ccatcggcag caagcgatac tcaacaagcg ttgctgccgg
     5881 agatcgccgg gccttcgctg gtcgagcctg caaatcggga gctcggtctc gcgctcgatg
     5941 gcgccttcgc cgagcccggg cagccgccgt tccgccacac caaggcggtg gtcgtactga
     6001 aggacggccg ggtcatcgcg gagctctacg cggccggcta tggcatcgac acgccgatgc
     6061 tcggctattc cgcgaccaag tcggtgacct cagccctgat cggaattctc gttcggcaag
     6121 gcaagcttgt gatcaacgag ccggccccca ttgcggcgtg gcaaagtccg cacgatcccc
     6181 gccatgcgat cacggtcgat catctgctgc gccacaccgc cggactggcg atgggcagtt
     6241 cgctcaatgc ctcgcttgcc agcgccttcg accgtgtcaa ccggatgaaa tatgtcgagc
     6301 gcgacatggc gggattcgcc gaaaacgccg aactggagag cgcgccgggc agtgtctgga
     6361 attaccacga cggcaacaca gtcattctgt cgcgcctgat ccgcgacgcg gcaggcggtc
     6421 atgccgccga tgtgctgcgc ctggcgcggg ctgagttgtt cgatcccttg gggatgcgcg
     6481 gcgtcacgct ggaattcgat gcgaccggca ccccggaggg atcgagccag atgctcgccc
     6541 cggcgcgcga ctgggcccgc tttggcctgc tttatctcaa cgatggcgtc gtcggcggca
     6601 ggcgcattct gcccgagggt tgggtccgct attcggcctc gctcacccca ggcgcctggg
     6661 cgggcttggg cgcgggattc tggaccaatc tcggcgacag caagggttcg cggtaccgta
     6721 cctcgctcgg catgccgcgc gatgcctttt atgccagcgg cgtgctcggg caatacgtca
     6781 ttatcgtgcc gtcggaacga ctggtcattc gcgcttcggc atctccggaa atcagtacga
     6841 catcgcgggc gtttcacgtt tggttgccga cgtgatcgcc gcaaccagta acaacgggcg
     6901 cgtcgcccgc ggcgattgag ccgagcatcg cgctcgtacc gtcgaagcgc ggtcccggtt
     6961 tgctaattgg tcctggcctc atagataggc ggtggcaaag cttagcatgc aagatcgcga
     7021 aaaatggcac gtccggatac caagaacacg acaaaatact ggtgggacag gcacatttca
     7081 ggtctcagcc tgatgcacgc cgacttcacg tgccacgatt ttgccccgca tacccatgat
     7141 gcatttgtca tcgccgtcac ggagcttggc ggtgcccgaa taaaaagtcg gggcgttacc
     7201 gatgtcgcgc gctcatccag actgtttgtt tccaatccgg aggagccgca atcctcctgg
     7261 atgggagaca gtccccgctg gcgttaccgg tcgatgtatc tgacgcaagc ggcgatcgac
     7321 gtcgtcgcag aggggcttgg gatcgaatcg gtgccgtttt tcaccggcaa catgatcggc
     7381 gatgctgatc tggtgagcgg ctttgcgtca ctccaccagg cactcgaagt tggagccgat
     7441 cgtttccgcg agcacgaact tctgatatcc gcattcggtc atctgttcgg ccgtcgcggg
     7501 agcgggggta atcgcatcga gcctgccccc cgcgacagca tccttgttcg caggatcgcc
     7561 gatttggtgc gggagcgatt tgccgaaaat cttcttctcg gcgatctcgc cgccgctgcc
     7621 ggattatcca ttttccagct gatcggactg ttcaagcgct cgatcggaac cacccctcac
     7681 gtttatctta cccatgtccg gctgaatgcg gcctgccgcc gtctcaggct cggagattct
     7741 atcgccgacg cagccgccgc ggccgggttt tgcgatcaga gtgcgctgac gaagcatctc
     7801 aagcgctgct acggcatcac gccacggcag tttgccaaag ctgccgcttc cgcccggctt
     7861 ctacggcgag ccccgcctgg ggctgcgcgg aatcctgggt agccttgcaa tttcagccaa
     7921 tacgcccaag accggatcgc ctagaaagtc ttgtcgtttg cagcttgaag cgaggtcatg
     7981 ataccacctt catcccggaa agcgcgttcc accgtcgccg tcattggcct cggaagcata
     8041 gggggcgtcg tggcgggatg tctgcgcgcg gcggatcggc atgacgtcat cgccggcgtc
     8101 cgacagccga tcgaacgtct tgtgctggag cgaccggaag ggaacgttga ggttgctctg
     8161 cgcgccttta cggacccagc gcaggcaggg cccgtcgatt gggttctgtt gtgcaccaaa
     8221 acccagcaga cgccgttgac cgctccgtgg ctggcacgat tttgcgattc gtcgacacgg
     8281 gtcgctgttc tacaaaatgg aatcggtcat cttgcccacg tcgcgccttt ggttggagat
     8341 gccggcattg tcccggccct ggtctactat aacggcgagc ggctaggcgc tgaccgcgtt
     8401 cgcctgcgcc acgttgccga gcatgacctc gccgtggcgg atgatgcgaa cggtcgcgcg
     8461 tttgcggagc tgctcgacgg cacgccgctc cgcgttcttc tgagcagcga tttcccgacg
     8521 ctggcgtggc ggaagttgct gatcaatgcc gttgccaatc caatcacggc gctcacgttg
     8581 cagcgacagg cggtgtttcg gcgcgacgac atcaaggcgt tgtgtgttgc aatcctcgat
     8641 gaagcggtcg cggtggcgcg cgccgacggc gcgcaaatgg ccgaggacga atcactgcga
     8701 accatatcgg cgcttcttac ttatccgccc gaggcgggca cctcgatgta cttcgatcgt
     8761 ctggcggggc gggcactcga agtcgaggcc ctgacgggcg ccgttgtcgc cgccggcgag
     8821 cgcctgcgga ttgcgacacc gctcaatggc gcgttgctgg ccttgctgcg tgcggtcagc
     8881 gatgccgcgc gcactggata gacgcgccct actgccgtcc cagctgcgtg gctttctcga
     8941 cgcgatcgaa cagctccagc gacaggatcg cgtcggcgaa ctggtcggcg cggccgttca
     9001 gcaccgggcg ggcgagcgtg aggaatttct gcgccatcgc ctgcgcgtcc gggaacgaat
     9061 tcggctcgcc ggaggggtcg gcatggagcc gctcatgcac gccatcgtcg gtggtgatcg
     9121 aaacccgggc gccgaacgga tggctgcggc cgatttcaag gcggtcgtcc tgcaccacat
     9181 cgaacttgtc cgccagcgcg tccaccgcgg tgtcgccaag cctttgataa tcgtcccagc
     9241 caaagcggcc ctgatcgagc gccaccgccc cggtaaagaa catcgagaac tggccgccga
     9301 cgatggaggt cggatgccgc ttggtggcgg catcgccggt cagcgtgatg ccgttacgat
     9361 gcagtccgat ctcgacacgt ttgatctggt ccggcgtcag attgtgctcc cggcgcatcg
     9421 cgatcagtgc atcgagggct gcgtgggtat agcggcagct cggatacggc ttcacgccga
     9481 tcttcatggt ctcgtacatt tcgccgaggc cggcgaccac cttgtcgggg tgcgcgttat
     9541 cgctgtaacc gaccaggagg ccgtgctggc cctcgaccga ttcggtcgcg ccgacaaaat
     9601 cgttgcgcgc cagggtggcc gcgatcacgc cattcatcgc ggcggcgcca acctggtagc
     9661 gcttgttcca ggcgccgttg accagaaatt gcagggagcc cgcggcctga ctgccggaga
     9721 cgccaaacgc cgcgatgatc tgttgtttcg acagtccgaa cagctttgcc gccgcggcgg
     9781 cagcgccgta ggttcccgcg gtcgcggtgg ggtgaaagcc gcgcgcataa tgcgaggtcg
     9841 gatcgagcgc gttgccgagc cggcagcaca cctcgtagcc cgccacgatc gcggtcagca
     9901 cgtcacggcc ggaagcgccg accagttcac cgaccgcgaa cgcggccggc accaccggcg
     9961 cgcttggatg cagcgacgaa tccgcgtggg tgtcgtcgaa atcgagcgaa tgcccgagcg
    10021 cgccattgag caatgccgcc accgccggtg tccaggtctt ggcgtcgccg aacacggtgg
    10081 actggccctt gccgtcgagc gcgagagcct cgagcatctt gagcagcgaa ggcgtcgatt
    10141 cggcgtcgcg tcgcgcccga atggcgctgc cgagaaaatc cagcgtcagg accttggcgc
    10201 ggtcgagcac ttcatgcgga atatccgcga atttcagatc cgcgacatag gcggcaagcg
    10261 ttgcggtttc ttctgccatc gtgtttcctc gctgttatcg cacagggtag gcgggctgat
    10321 ttgccgtttc aagccgctcg cgcgaggcgg gaagcagcca tgccaaatgt cctttataag
    10381 ggggccttgg tcactcacat tccggaacgg ctgcatgatc gccttcttga aacgcctatc
    10441 gcgcttgcta cggtcccgga gggcgcaact ggcgttggcc ttcagggtta ccgtcgcggc
    10501 cgccggtgcc tacgcgatcg caacggcgat acatctgagg ctgccgttat gggcggtgct
    10561 gacgtccctt attgtaaccc agatgagcgt cggccgatcg ctaaaggcga cccgtgacta
    10621 tatgttcggc acagtgggcg gcgcgatcta tggcggcgcc atcgcggtgt tgattccgca
    10681 ttccggggag gtcgaattgc tcggcttgct ggtgctcgcg gttgcaccgc tggctttcat
    10741 cgccgccaac aatcccagcc tgaacgcagc cacggtcact gccgtgatcg tgctgctggt
    10801 tccgatcatg agccacgtcg atcccctcgc ttccgcgatc gatcgtgtga tggaagtgac
    10861 ggtcggcgcg ctcaccgggc tcctggtaac atttttcgtg ctgccgtcgc gggcgcacaa
    10921 ccagatccgt attactgcca cgcggacgct cgagttgatc gcagtggcgt tctcaaagct
    10981 gctggacggc ctgacccatg gcctcgacaa cgaggccttg catcggattc aggatggcat
    11041 cggcgcagcg ttggtgggct tgaatgcgac cggcgcggaa gccgagcgcg agcgcgcggc
    11101 gcatctgtcc cgggggccgg ataccggtcc gctcctgcgg acctcattgc ggctccggca
    11161 cgacctcgtg atgatcgggc gtgcctgcat gatgtcgttg ccatcggatt tgcaggcgcg
    11221 gctcgcggtg ccgctcgaca aagtcagcga tgcggttgtg acctatttgc gttccgccgc
    11281 agcttcattg cgaacccgcg gcaattctcc gtcgtcctcg ccgatcgacg aggcactgca
    11341 gctctatgcc gcggaagtcg cagcgctgcg cagcgaaggc ttgatacgcg tcctgcccgg
    11401 cgacgcggcg gagcgcttct tcgcgctagg atttgcgctg gagcaaatgc ggcagaatct
    11461 gaacgacctc ggccgctgcg tcgcggagtg gtccgaaacg ccgccttgac ctgtaggacg
    11521 ctgcgctagt cgagatagac accgaggaag ctcagcatgg ccgtgtcgca ctgcgcgatg
    11581 ccgtcggtga gcgccttgag ggcggcgtcg aagttcggcg aggggtgttc cagttcccat
    11641 gcgccatcgt ggtcccagtg gaagcaccag ccaaaacggt cgcctttctc caggtgaatc
    11701 accacgcagc ggctctcctg cgcgggttcg gcaaccggga cgagatcgcg aagcgaaacg
    11761 ccgtactcct tgcggaaata ggccgagacc ttttcgcgat ttttttcagt gcgcgtgatg
    11821 aacttgaaca ggttgtcgcg ctgcgtggcc agttccaacg gcttgtagaa gcggagttca
    11881 ccggagtggc cgggtaaccg gaccaacagc tcggtctcgc cataggtggt gaggaacttg
    11941 cgatagtcct tgggcaactc cgccccaagc tgccgctcgg cttcatccac gtcttgcttg
    12001 cccgccggct tcttcacctt ccagcgccga cccttgtcgg atttcgcctg gttcttgcgc
    12061 cgctcgacat acgcggcata ggccggcagc gcggtgatcg cctcgtaacc gccatgcatg
    12121 acccatcgga aggcggcgtc gaaggcttcc tcccgccgct ccggataatg tttcgcacag
    12181 gatggtcgcg cactgtaccc tgcggtccga gcctggcgcg atcttgcaag cttcttcgat
    12241 tgcttcgagc gcggcggcag gacggcccgc gcgctgatgg gtccagctta gaacgtcgag
    12301 gatgcggcct tccaggtcgg atggtgcgat cgaccacgca taatgcggcg tcttgccggc
    12361 cttgcgggac gtcagcatat cacgggcctg gttggcgaag cggatggttc gttcgtcatt
    12421 gtcggcggcg gcgtagctgt gatgcgccgc gagccagaga taaagcggct cgcgcgccgc
    12481 cggggtattt tcgagcgccg caagatgctt tgccgctgat cgcgagcgtt gtccaggctt
    12541 cgcagagttc agtcgctggt ttggggatgc cggttcaatg cttgcgttca agtcttgcgg
    12601 cgggtcaaga acgcaccggc gaagagcagc tcggcctggc tcgccggatt taggcttgcg
    12661 cctgcaccac accacatatt cggcatgagc cggcagcgcg gtgatcttcc tcgcggccat
    12721 caagctctgc ccgtttattg gcggcggcga gcgcttcctg ccgccgatcc ggaaagtgat
    12781 cgcacaggat tgtggcgcgc tggacgccgc ggtcgttgct tggcgcgatc ctgtaagcct
    12841 gctcgatcgc atcgagcgcg gccgcgggat tttccgcgcg cagatgggcc caactcagaa
    12901 cgtcgaaaat ccgggcttcc aggtcgtttt cggcaatcga ccatgcgtaa tgaggcgtct
    12961 tttctgcccg gcgcaaggcc agcgtgtcgc gcgccgcttc ggcaagctgg attgtgtgtt
    13021 catggctgtc ggcagcggcg aagctgcgat gtgccgccag ccacagatag agcggctcgt
    13081 gcgccgcagg ggtgtcttgg agcgcggcaa ggtgcctcgc ctggtcttca agtgaagcgc
    13141 tgagtgctgc gagcatcagg tcgtcgaggc tcttttgcca ttcaagcttt gcttccggac
    13201 agcgcagatt gcgccgcgta tgtcgggtgt gcgtggtttc catataaccc gccgcgacaa
    13261 gctcggccgc gtaacggcga taggcggcga ccacattgcc gatctcggaa aatggcacgc
    13321 gcaggaaatc ttcagtctgg atttcgacgc tcccgcgcat gcggaagccg atgatcattg
    13381 gatcgccgcc cggaaccaaa agcagctccc ggtcatcatc acccggcggg cgatgaaaga
    13441 attttgcaag atacatcaag gtctccggac gcccggccgc acgcccgcgc tgtcggtggg
    13501 gagcgtcgtc cagactgtgc agagtcagtc gctggctcgg ggattccggt tcaagacttt
    13561 gcgcgtagga cttgcgttca agtcttgcgt tcaaggcttt gcgttcaaga cttgcggcgg
    13621 gtcagcccct tgtcttgcat ccgccagatc agcaggtcga tgcgatcctg gccgaagaac
    13681 ggctcgttct cgaacacgaa agtcggcacg ccccagtggc cggacgcggc gtggtccttt
    13741 tcgttatcag caatgatccg ctcataggaa tcgggatcgg ccgatatcgc agcatccatc
    13801 gcggccaaat cgaagccggc tgccgccgcg gcgtgcgcga gatggtcgcc ctcgttccag
    13861 ccgctgacag tgccgtccca caacacccgg ctgatcgcat cggcaaatcc gagcgagcgg
    13921 ccctcacgct gcgcggcggc gcccaggcgc gtcagtcggt ggatgtaggg ctggttctca
    13981 gccacatcga gcgtcgtcat gtcctgcacg atcgggtcgg gtcggggaaa cctgaacggg
    14041 atgttttcgc ggcgggcaac gcggctggag tcgagcacga cgtaacgcgc aaactgcgga
    14101 ttggtgcgct tgaaaaaacc gggcacgcga acggcgagcg ggtagaccgg gcgcagggtc
    14161 accgtcacgt cgtagttggc ggccagcctg agcgttttcg gcagcgccaa atagctgtag
    14221 gggctgcgga atgaaaagaa cagatcgacg gacaatgtca tggcaagacc tggccggcat
    14281 atttggctga cggcaagaaa gcatgggccg ggcaacgtgt ccacgcggca gcccgcggat
    14341 gttcgtctca gcctgcaact tgctgcagca tcgcgcggac gacgagacgg tgaaacggca
    14401 agatcatcgc gagataggcc cggccgagca gattgtgcgt cagcacgagc gtggtcgcgg
    14461 tgacgcggcg gccttggcca aaggctgtga cgtcgaccac gacgcgaaaa tccaggtgct
    14521 tgtcgttaaa gccggcaacg aggcgatccg gcgtctcgct caggatcgga aatagcccga
    14581 tggcgttggc gggcgcgatc tcgctcggca aggacgtttt caagccgaat ggcgtcacca
    14641 gagtggcgca aggccagcaa cgcctcgatc catcgcggcg atcgccccaa catccgctcc
    14701 gcggcatgcc gcgcatcgag cgctgtgtca tcaacggcga tgctgtaggc gtcgcaaaac
    14761 tgcgcgccag ccagcagcgc gccggtgtca atgttgggct cgaccttgcc gactttcatg
    14821 cttgtcgcct gagttacaga actgacgtct ctcatcatgc atccacgctc cggtagttat
    14881 gcggggccgc caataaaccg ttgaatagcg tcatggcgct atccatggag ccagggaaga
    14941 atcgtctgcg cgctggtccc gtgtgcgagg catgccgctc attgtcaagc gcggctgcgg
    15001 tcccgacaac tgactggctt cggactgtcg ggtcagcggc tgaacaataa cgcagctggg
    15061 ttgccggaga gttttttgct ctgcggtgcg gcgctgccgg gccatttcag gcgaaccttg
    15121 agaaaaatca cctgagcggc gaggaatccg ccaaggcgat gaatctcagc gccaaggcgt
    15181 tcgaatctct gctggcgcgg gcacgggcga cgctgagaga gcacgtccgg aaaattcaga
    15241 ataccaggag gtgcgcatga cgctcgatca gttcaggcaa ttggccgaga cttggggtgg
    15301 ggatatcgat cgctggccga ccgccacgca agacgcggcg cgcgagatcg cgaccagcag
    15361 cgaaggcggg cgcatcttga aggaacagct tcggctggat cgattgttcg cgatcgcccc
    15421 tgacgtaagg gaccaacggg cgggacggct cggctttgcg gttctgcagc aactggcgaa
    15481 gggggatcgc gatctgccgt ggtttcgtcg gctgtggcgc ccggcgtcgc tgtttcccgc
    15541 agccagtctc gccggctccg ccatggtggg tcggtggcgt caggatgttt tcccggccta
    15601 cggccggctt cgtgcgggca acccttgccg aggcgtgtct gatcggcctg ctggttctct
    15661 cattgtcgcg ggcgggcgca caggacgggc agggcggcgc gcgccaagcc tgtgaggcgg
    15721 attaccatcg cctctgcgcc ggcatcagtc ccggcggcgg ccgcatcaaa aaatgtctga
    15781 atgacaattt cgacgcgctg tcggatgcct gcaagcaggt cgttggcgca ggtaccggca
    15841 agtgagtttc agaggagcga cgatccctca cccggttgag aggctttggc ccgccgcggt
    15901 tggcctccac ctctaaccgg cgtcctgcgt tcgctccggc acgcgggtga tcctggcgcc
    15961 cagcgcgttg agccgttcgt cgatgcgctc atagccgcgt tcgatctgat cggcgttgtc
    16021 gatggtgctg acgcccttgg cgcacaccgc ggccagcagc atggccatgc cggcgcggat
    16081 gtcgggagat atcatcggcg cgcccttgag gcggctcggt ccggccacga tcgcccggtg
    16141 tggatcgcac agcacgatgc gcccacccat cgcgatcagc ttgtcgacga agaacatccg
    16201 cgattcgaac atcttctcga acatcaggat gacgccgtcg cactgggtcg cggtcacgat
    16261 ggcgatcgac atcagatcgg ccgggaaagc cggccatggc tgatcctcga gcttcggcac
    16321 atggccgccg aaatcgtccc ggatcttcag cgtctgaccg gacggcacca caaggtcgtc
    16381 gcagtcgacc tcgcagacta tgccgagccg ctcaaagccc atccggatcg agcgcagatg
    16441 ctcgatgccg gcgcgcgcga tgcgcagcgg cgaacgcgtc accgcggcga gcccaatcaa
    16501 cgagcccacc tcgatgtgat cggggccgat cgaataggtg gcggcgccga gcgtagccgg
    16561 cccgtgcacg accatggtgt tggtgccgat gccctcaatg cgggcgccca gcgccaccag
    16621 gaaatgcgcg agatcctgca catgcggctc ggacgcgacg ttgcgcaaat gactggtgcc
    16681 gtacgcggcc accgcggcca tcaatgcgtt ttccgtggcc gtcacgcttg gttcgtcgag
    16741 gaaaacgtcg gcgcccttga ggcggaggca gcggaattgc aggcgatcgt ttgcgaccac
    16801 ggaggcgccg agctgttcga aggccagaaa atgggtgtcg agacggcgcc ggccgatgac
    16861 atcgccgccc ggcggcggca gcatcacttc gccgcagcgg gcaagcagcg gtcccgccag
    16921 caggatcgag gcgcggatcc gcgcgcacag caccggatcg agatcggccg cgcgaatatt
    16981 cctggcgtgg atggcaagcg tgttgcgttg ccgccattcg gctgacgcgc cgaccgagcg
    17041 gatcagttcc acgagggttt cggtatcgcg aatgcgcgga acattctcaa gcgtaaccgg
    17101 atgctcggtc agcaatgccg cggcgatgat cggcaaggcg gcgttcttgt ttccggaggg
    17161 ctcgatggtg cccgccaggc ggtggccgcc ctcaacgatg tactggagcg gcacgcgggt
    17221 catacgtcca cattggcgct gagcgaattg tcctggatga attcgcggcg cggttcgacg
    17281 atatcaccca tcagcttggt gaagatgtcg tcggcctcgt cgacctcctt gatcttcacc
    17341 tgcagcagcg agcgctcgtt ggtgtcgagc gtggtttccc agagctggcc cgggttcatt
    17401 tcgccgaggc ctttgtagcg ctgcagcgcc acgcccttgc ggcccgcatc cgtcaccgcc
    17461 tcgaacaggc tgaccgggcc gtggatcggg gtttcggtat ccttccggcg cagcaggccc
    17521 ggcttgggat aggcttcctg cagccttgcg gcgtaatcgt cgagcttgcg cgcgtcggcg
    17581 gagccgagca gcgcgtcgtc gatgatggcg acatccttga cgccgcgcac ggtgcgctcg
    17641 aagaaaaacc cttcgccttc ggtgaagcgc ccggtccagc cgcgttcgac ttcatcggcc
    17701 agcgcgtcga gccgtcgcgc gatatagtcg gcggctgcat tggcggtcgg ggcatcgccg
    17761 gtgattttcg ggctgagcac gccggcgatc gcggcctgtt cgatcacctt gcggttatag
    17821 cggctgtgca gattgtgcag cgtgccgcgg atgattcggg cgtcctccac cagcgccagc
    17881 agatcgcggc cgccgcggtc ctcgccggag gcaagcttgt agacgcagtc gtcgaggccg
    17941 gtcgcgatca gataatcctc cagcgcgcgc tcgtccttca gatattgctc ggacttgccg
    18001 cgcgtcacct tgtagagcgg cggctgggcg atatagaggt agccgccctc gatcagcgcc
    18061 ggcatctgac ggaagaagaa cgtcagcagc agggtgcgga tatgcgcgcc gtcgacgtcg
    18121 gcgtcggtca tcaggatgat cttgtggtag cgcagtttga cgagatcgaa atcgtcgcgg
    18181 ccgatgccgg tgcccaacgc ggtgatcagc gtgccgatct gctcggagcc cagcatcttg
    18241 tcgaagcggg cgcgctcgac attgaggatc ttgccgcgca acggcagcac cgcctggaat
    18301 tcgcggttgc ggccctgctt ggcactgccg ccggcggaat caccctcgac gatgaacagt
    18361 tcggatttgg cgggatcgcg ctcctggcag tcggcgagct tgcccggcag cgaggcgatg
    18421 tcgagcgcgc ccttgcgccg cgtcagctcg cgcgccttgc gcgcggcttc gcgggcggcg
    18481 gcggcttcca ccaccttgcc gacaatgacc ttggcttccg cgggatgttc ctcgaaccac
    18541 gccgcca
//
LOCUS       DATNOI010000391         4282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2297926,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000391 DATNOI010000000
VERSION     DATNOI010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2297926"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VN968_15595"
     CDS             <1..272
                     /locus_tag="VN968_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA ligase-associated DEXH box helicase"
                     /protein_id="HXL30693.1"
                     /translation="IYDVLRKHQPDHVLLRGARADAATGLLDLRRLGDMLLRIKGRIV
                     HKELDKVSPLAVPVMLEIGRESVYGEASDELLAEAADELVKEAMS"
     gene            285..980
                     /gene="pdeM"
                     /locus_tag="VN968_15600"
     CDS             285..980
                     /gene="pdeM"
                     /locus_tag="VN968_15600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HXL30694.1"
                     /translation="MTRTAIDIVHRSSLDVVGVDLVADLSGALFWEEQRLLVVSDLHL
                     EKGSSFAARGVLLPPYDTVATLGRLAAVIARHDPRMVIALGDSFHDRTAHERLSDSDR
                     DALSALQVRRDWIWISGNHDPALPSDLGGVVATEVAIGPIAFRHEPTGAAGEIAGHLH
                     PKARVSTRGRSMERRCFACDGERAVMPAFGAYTGGLSIRDAAFAKIFQTLGFMAHVLG
                     DVRLHTIAASRCY"
     gene            complement(1086..1949)
                     /locus_tag="VN968_15605"
     CDS             complement(1086..1949)
                     /locus_tag="VN968_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL30695.1"
                     /translation="MLDLELLRSFVSVVDSGGFTRAGERVHRTQSTVSQQIKRLEDDL
                     GQPLLNRTAKDVTPTEAGERLLSYARRLLSLAEEARDVLAGPSKEGAVRLGIPEDFAA
                     YRLTKLLASFSRSRPGLRLDVRVDQSTYLRRDLERGDLDLALFKRSAGEKGGIAVWPE
                     RVHWVTSKTHPIGAQIRSVPLIGFPTGCLYRSRAIHAIESAGRAWHMAYTSSSLTGIQ
                     AAVAAGLGLSILSEIAIQADHRVLTAKDGFAPIDKTEVALVASPDASPATLRLADRLA
                     EFCNTVQAKAA"
     gene            2049..2963
                     /locus_tag="VN968_15610"
     CDS             2049..2963
                     /locus_tag="VN968_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXL30696.1"
                     /translation="MSLAPSIAVPRASFNPLPLYIGLFCLLWSFAFVAGKIGVTDCPP
                     LILLTARFSLAGILILGVSALRRDAWSLSWRDTFAFAILGIANNALYLGLGYTGLQTV
                     SAGLGGLIVSANPVFTAGLAALFLGESLTWRKVAGLLLGITGVSFIVWHRMSVGTDSW
                     HGIVFTLASLASIVAGTILFKLFAPKGSLWIGNGVQNLAAGVVLLPFAFTLADVGDIV
                     PSTRLLGAFAFLTLGGSILAYLLWFHLLKVCGATTASAYHFLMPPLGMLFAWIVLGEH
                     VEVRDLMGIVPVALGIYLVTRPAAASAT"
     gene            2976..3758
                     /locus_tag="VN968_15615"
     CDS             2976..3758
                     /locus_tag="VN968_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL30697.1"
                     /translation="MNSLRITLIGGPTALIEVDGFRLLTDPTFDAPGAYQLPHVTLEK
                     LSGPALGAEAIGEVDAVLLSHDQHADNLDHSGKASLARAKRVLTTVAGAKRLAGHAEG
                     LMPWATTELTSKDGHALTITATPARHGPAGIEPLSGDVIGFVLTSKDKSPPIYISGDT
                     VWYDGVAEVARRFKAGVVLPFAGAAQTRGPFHLTMDTNDTIEIARAFPDAVIVPLHTD
                     GWAHFRQNAKDLRASFDTLGFGSRLKILEPGVATVIEPAHRS"
     gene            complement(3832..>4282)
                     /locus_tag="VN968_15620"
     CDS             complement(3832..>4282)
                     /locus_tag="VN968_15620"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXL30698.1"
                     /translation="GGGCQYLLVTDYNIAAKDAYMTLPARKEGIIPGAANMRLPRFVG
                     DRVTRQAIMSELRMECDSATGRMICDRIVEPEQMDQAIDEAVELLTNSGAVNASSNRK
                     AIRVAQEPLDVFRTYMAVYAREQAYCHFSPALVSNLERFWNAQQRKL"
BASE COUNT          724 a         1331 c         1316 g          911 t
ORIGIN      
        1 tgatctacga cgtgctgcgc aagcatcagc cggatcatgt gctattgcgt ggcgcgcgcg
       61 ccgacgccgc gaccgggttg ctcgatcttc gccggctcgg tgatatgctg ctgcgaatca
      121 agggacgcat tgtccacaag gaactcgaca aggtttcgcc gctggcggtg ccggtgatgc
      181 tggaaatcgg ccgtgaatca gtttatggcg aagcatccga cgagttgctg gcggaagccg
      241 ccgacgaact ggtcaaagag gcgatgtcgt agaataaaat ggccatgacg agaactgcta
      301 tagatatcgt tcatcgctct tcattggacg ttgtcggtgt agatttagtt gccgatctct
      361 ccggtgccct gttctgggaa gagcagcgcc tgctcgtggt ctcggatctg catctggaaa
      421 aaggctccag cttcgccgcg cgcggcgtgc tgctgccgcc ttacgatacg gtggcgacgc
      481 tcggtcgcct tgccgctgtc atcgcgcggc acgatccgcg gatggtgatt gcgctcggcg
      541 acagttttca tgaccgcacc gcccatgagc ggctgtcaga ctccgaccgc gatgcgctct
      601 cggcgttgca ggtgcggcgc gactggatct ggatttcggg caatcacgac cccgcgctgc
      661 cgtccgatct cggaggtgtg gttgccaccg aagtcgctat tggcccgatc gcgtttcgtc
      721 acgagccgac cggagcagca ggcgaaatcg ccggacatct gcatcctaaa gcgcgggtgt
      781 cgacacgcgg gcgttcgatg gagcggcgat gttttgcttg cgacggcgag cgcgccgtga
      841 tgcccgcgtt cggcgcctat accggcggcc tcagtatccg cgacgcggcg ttcgcgaaaa
      901 tattccagac cctgggcttc atggcgcatg tgctcggcga tgtcaggctg cacaccatcg
      961 ccgcctcgcg gtgttactga tttaatagtc taatttaact gcgagcattc ttcacctctc
     1021 cccttgtgtg cacgccttag cccacaaggg gagaggggtg caccttccgt gctgcaagac
     1081 taacgctacg ccgccttcgc ctgcacggtg ttgcagaatt ccgcaaggcg atccgctagc
     1141 cgcagcgtcg cggggctggc gtcgggggaa gccaccagcg ccacttcggt cttgtcgatc
     1201 ggtgcgaagc cgtcctttgc ggtcagcacg cgatggtcgg cctggatcgc tatctcggag
     1261 aggatgctca agcccaatcc ggcggcgacc gcggcttgaa tgccggtgag gcttgaactg
     1321 gtataggcca tgtgccacgc gcgcccggcg ctctcgatgg catggatcgc gcggctccgg
     1381 tacaggcagc cggtgggaaa accgatcaac ggtaccgagc ggatttgggc accgatcgga
     1441 tgggtcttgc tggtgaccca gtgcacgcgc tccggccaca ccgcgatgcc gcctttctcg
     1501 cccgcgctgc gcttgaacaa tgcgaggtca agatcaccgc gctccagatc gcgccgcaga
     1561 taggtgctct gatcgacgcg gacgtcgagt cgcagcccgg ggcgcgagcg ggaaaaactc
     1621 gccagcaatt ttgtcaggcg ataggccgca aaatcctcgg ggatgccgag gcgcaccgcg
     1681 ccttccttgc tcggtcccgc caatacgtcg cgggcttcct ccgcgagcga caataaccgt
     1741 cgcgcatagg atagcaggcg ttcgccggct tcggtcggcg tcacgtcctt agccgtacgg
     1801 ttcaggagcg gctggccgag atcgtcctcc agccgcttga tctgctggct gacggtggat
     1861 tgggtgcggt gaacgcgttc cccggcgcgc gtaaagccgc cggaatcgac caccgagacg
     1921 aaactgcgca aaagctccag atcgagcatg gcgatggctc atttatgttt ccactgacaa
     1981 ccagtttatc atttaattta caaatatcaa ggcgcgggcc tacatcggtg gtcacaagga
     2041 gacttcctat gtcgctcgcg ccatcgattg cggttccccg cgccagtttt aatccgctgc
     2101 cgctctatat cggcctgttc tgcctgctgt ggagctttgc ctttgtcgcc ggcaagatcg
     2161 gtgtcaccga ttgtccgccg ctgatcctgc tcaccgcgcg tttttcgctg gcgggaattt
     2221 tgatcctggg agtttctgcg ttgcgccgcg acgcctggtc gctgtcatgg cgcgacacct
     2281 tcgcatttgc gattctgggc atcgccaaca acgcgctcta tctagggctc ggctacaccg
     2341 ggctgcagac cgtgtcggcc ggcctcggcg gcctgatcgt cagcgccaat ccggtgttca
     2401 ccgcaggtct ggccgcgctt tttctcgggg agtccctgac ctggcgcaag gtggcggggc
     2461 tgctgctcgg catcaccggc gtcagcttta tcgtctggca tcggatgtca gtcggcaccg
     2521 atagctggca cggcatcgtg ttcacgctcg cttcactggc gtcgattgtg gccggcacca
     2581 tcctgttcaa actgttcgct ccgaagggca gcctctggat cggcaacggc gtgcagaatc
     2641 tggctgcggg cgtcgtgctc ctcccgttcg cgttcaccct tgcggatgta ggcgatatcg
     2701 tgccgagcac gcgtctcctc ggtgcattcg catttcttac gctcggcggc tcgatcctgg
     2761 cctatctgct gtggttccat ctcttgaagg tgtgcggagc cacgaccgca agcgcctatc
     2821 acttcctgat gccgccgctc ggcatgctgt tcgcctggat cgtgcttggc gagcatgtcg
     2881 aagtcaggga tttaatgggg attgttccgg tggcgctcgg catttatctg gtgacacgtc
     2941 ccgccgcagc gtcggcgaca taaagcgagg tccccatgaa ttcgcttcgg attacactga
     3001 tcggcggccc caccgcgctt attgaggtcg acggctttcg gcttctcacc gatccgacct
     3061 ttgatgcgcc cggcgcttat caattgccgc atgtgacgct tgaaaaacta tccggccccg
     3121 cgctcggcgc cgaggcgatc ggcgaagtcg atgccgtgct gctcagtcac gaccagcacg
     3181 cggacaatct cgatcattcg ggtaaggcgt ctctcgccag ggccaaacgc gtcctgacaa
     3241 cggttgcagg cgccaaacgg ctcgccggcc atgccgaagg cctcatgcca tgggcaacca
     3301 ccgaattgac cagcaaggac gggcatgcgt tgacgatcac cgcgacaccc gcgcgtcatg
     3361 gtcccgccgg tatcgagccg ctgtccggtg atgtcattgg cttcgtgctg acctcgaaag
     3421 acaagagccc gccgatctac atcagcggcg acaccgtctg gtacgacggc gtggcggagg
     3481 tcgcccggcg attcaaggcg ggcgtggtgc tgccgttcgc gggcgcggcg cagacccgcg
     3541 gcccgttcca tctcaccatg gataccaacg acaccattga aatcgcgcgc gcgtttccgg
     3601 acgccgtgat cgtaccgttg cacaccgatg gctgggcgca cttccgtcag aacgcaaagg
     3661 atctgcgcgc gtcattcgat acgcttgggt ttggctcgcg tctgaaaatc ctcgagcccg
     3721 gagtcgccac cgtcatcgag ccggcgcatc gctcttaaat ggcgttggca acggccagtg
     3781 agcttgggtt tctcttatcg tcgcaccatt caagccgcct gcttccgccg cttagagttt
     3841 tcgctgctgg gcgttccaga agcgctcgag attgctgaca agtgccggtg aaaagtgaca
     3901 gtaagcctgt tcgcgggcgt ataccgccat ataggtcctg aacacatcga gcggttcctg
     3961 agcgacccgg atcgccttgc ggttacttga tgcgttcact gcgcccgaat ttgtcagcaa
     4021 ttcgacggcc tcgtcgatcg cctggtccat ctgttccggc tcgacaatac ggtcgcagat
     4081 catccgtccg gtagcgctgt cgcactccat tcgaagttcg ctcatgatgg cttgacgcgt
     4141 aacgcgatct ccgacgaagc ggggcagccg catgttggcg gcgccaggga tgatcccttc
     4201 cttgcgcgcc ggaagcgtca tgtaggcatc tttcgccgca atgttgtagt cggtcacaag
     4261 caggtactga catccgcctc ca
//
LOCUS       DATNOI010000392        23697 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_962095,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000392 DATNOI010000000
VERSION     DATNOI010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 23697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23697
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962095"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VN968_15625"
     CDS             complement(<1..131)
                     /locus_tag="VN968_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HXL30699.1"
                     /translation="MDGGAVRASPDASSAAQAIYATAPPRLLQIRTLVADAGRQTSIG
                     "
     gene            complement(288..797)
                     /locus_tag="VN968_15630"
     CDS             complement(288..797)
                     /locus_tag="VN968_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2846 domain-containing protein"
                     /protein_id="HXL30700.1"
                     /translation="MLSLAAALLLGCASGRIGADYATMIQKVGSPRAGQARMVLLQEK
                     ASGLGYGSCACDMKLDGDPIGRLKPGTYLYADRPAGHHQLLAAETMFPGDSKRDITME
                     SGRTYFFLARVSERHSTLTGMTVAGGLAGMLVASAVTSGSENPGPVDFFPLDEPGARA
                     TLAELQLAE"
     gene            complement(1056..3359)
                     /locus_tag="VN968_15635"
     CDS             complement(1056..3359)
                     /locus_tag="VN968_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXL30701.1"
                     /translation="MRVTRNFSILSFCLILLCGFPGILSSAAAQQQEKRIALVVGDGA
                     YAKSPLATAANDAGLIAQTLQAAGFDVVGARDLDGDTLRRSFRDFIQKAEASGPDTVA
                     MVYLAGYGMQLAGENYFIPVDSPISRDTDIPTEGMRINDYVRQLASLPLKAGVVVLDA
                     ARQQPFVEGGQPLASGLALVEPDPHMLIAFNAAPGTIAPAEQGPYGVYAQSLAEMIRT
                     GGLSLPEVFNRVRLRVNETSKGAQVPWDAEKIDGQFMFFNRAPDAPAPAAPDQVAAVR
                     GKPIRDLGVQDAYAASLERDTMQGYEDFLTAYPNDPLAKRVRAIAAARREAITWLRSY
                     RADTPQAYWSYLRRYPRGPHAGDARRRLAILIASLEPPPSFAVIDYDVPPPPPEEIVY
                     VDRPMLMFSDPEFDFAPPPPPPLFFLPAPPADFVVLEPPPPPIGLFILPQPFFVPIPV
                     YIQQPAYVLPPPNNIIFANIHNTTVINNVINRPPQPGAGNVQPNAKGVAASPGVKGAP
                     GQGGVTQSGTGPALPPAVAQRASLIQQGKLAVPPSASINPAARLGTPGTPSGQKAAPN
                     TPLPANLSPNALPQGQALPRANALPVPGAKGTPPAPPGATVPAPVAPNPNAKLAPQPR
                     ANEPAFRAGQPKAGGSAQAPEAKPVVPETVRRPNAAPPPPPPPKPVAAPPRPAPPAAP
                     RPQMQRAEPPHRAAPPPRPAPQMARPAPPPPRMAAPPPPRPAPPPPRPAPQMARPAPP
                     PPRPAPPQPRAAAPAAAKKCPPNVPRC"
     gene            3633..4301
                     /locus_tag="VN968_15640"
     CDS             3633..4301
                     /locus_tag="VN968_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HXL30702.1"
                     /translation="MATCTIGDLSRREILKMSAITGVASVVSTRFAMARTVVPRTPEQ
                     ILGPFYPLKPLDQNADLTRVSGRPGRAEGQVLIVTGRVLNLNGEPVRNAKIEIWQANA
                     HGRYTHPSDTNPAPLDPNFEGAAVLTADDDGRYRFETIKPAAYPVGPHLMRPAHIHFQ
                     VSGRQDRLVTQMYFEGDPYNATDRILQTAGRTELLIAKLLPPTPEMEPKSKLAVFDIV
                     LYAG"
     gene            complement(4453..7965)
                     /gene="dnaE"
                     /locus_tag="VN968_15645"
     CDS             complement(4453..7965)
                     /gene="dnaE"
                     /locus_tag="VN968_15645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXL30703.1"
                     /translation="MSSAGFVHLHVHSAYSLLKGSIKIQKLGELAKADRQPALALTDT
                     DNMFGALEFSDKMAGYGIQPIIGCELAVDFGDQDPSARNALAAAPARIVLLAARERGY
                     RSLMRLNSRAFLETPVHQSPHIKFEWLQGDAEDLIALTGGPEGPIALAINADQAALAA
                     TRCDRMANLFGDRLYIELQRHGIEKERRAEAGLIDLAYAKGLPLVATNEPYFATADDY
                     EAHDALLCIAGGRLVAETDRDQLTPDHRFKTRAEMAVLFADVPEALASTIEIAERCSF
                     RPVTRKPILPRFTVGAGTNAVEAESDEAAELRRQAEQGLNDRLRVHGLSQGGSEEDYR
                     ARLAFEIDVITRMNYAGYFLIVADFIQWAKAEGIPVGPGRGSGAGSLVAWALTITDLD
                     PIRFGLLFERFLNPERVSMPDFDIDFCQDRRGEVIDYVQQRYGRDQVAQIITFGTLQA
                     RGVLRDVGRVLQMPYGQVDKLTKLVPQNPAAPVTLAAAIASEPKLQAFRDEDPVVARA
                     FDIAQRLEGLTRHASTHAAGIVIADRPLSELVALYRDPKSDMPVTQFNMKWVEPAGLV
                     KFDFLGLKTLTVLDVAVKLLRKRGVDVDLATLPLDDAASYQMLARGDVVGVFQVESQG
                     MRRALVDMRPDRFEDIIALVALYRPGPMANIPTYCARKHGDEEPEYLHPMLEPILKET
                     FGVIIYQEQVMQIAQVMAGYSLGEADLLRRAMGKKIRSEMEKQRAGFVAGAMKNNVPK
                     GQADTIFELLAKFADYGFNKSHAAAYALVSYHTAYMKAHYPVEFLAASMTLDLSNTDK
                     LSEFRAEAQRLGIKVEAPSVNRSGATFEVSEGTIYYALAGLKGVGGQAVELIVDARKD
                     GLFTSLADFAARVNPRAINKRVIESLAAAGAFDALDSNRARVFAGAEAILAACQRSHE
                     AATVGQNDMFGGAADAPTIMLPQVEPWLPAERLRREYDAIGFFLSGHPLDDYATVLKR
                     LRVQTWAEFSRAVKTGATAGKVAATVVSRMERRTKTGNKMGIMGLSDPTGHFEAVLFS
                     EGLAQYREVLEPGAAVLLQLGAELQGEDVRARVLHAEPLDAAAAKTQKGLRIFVRDTR
                     PLESIAKRLQMPEPAPQGGPVRGPQTKPLPDAAGGANGDVSLVMMLDLETEVEMKLPG
                     RFRVSPQIAGAIKAVSGVVDVQQL"
     gene            8624..9301
                     /locus_tag="VN968_15650"
     CDS             8624..9301
                     /locus_tag="VN968_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30704.1"
                     /translation="MSWRRRRIASGKPGVDSWHFIALAVAAIAIGAATYGIGWRSSHP
                     SGEAKSRPALLIENGSVQRDSKYFLALFGTYARSGKIARFYVSIQTDPGGNIQTAEFS
                     KPTLVKVLEVKDFEKGEPVHVAVASLRQEDSPQGPFRLGSSDTGLPSEAGFTTTSLVK
                     ATVIVKDDSDAEQTFGFVLIPRTTTMELMRANRSNLDRRAEDRQPVPVLNGDNLKLMT
                     ETDGDRQ"
     gene            9578..9856
                     /locus_tag="VN968_15655"
     CDS             9578..9856
                     /locus_tag="VN968_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HXL30705.1"
                     /translation="MRNLVLALLAAGGLAMIGAAPAQAVGTRYPFCIQGQDNPGLSNC
                     TFTSYEQCQASASGRFLYCVENPYYNPGEPDPRAYRSRHRVRSVYPSY"
     gene            9976..10236
                     /locus_tag="VN968_15660"
     CDS             9976..10236
                     /locus_tag="VN968_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HXL30706.1"
                     /translation="MREVFGALILCCAGSTLGALPAAARDYPFCIKGCDFGSGRGDCS
                     FSSYQQCQATASGLTATCAENPYFNAKAEWQPDRGRQSPRRY"
     gene            10236..10499
                     /locus_tag="VN968_15665"
     CDS             10236..10499
                     /locus_tag="VN968_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HXL30707.1"
                     /translation="MRSLFFATMAIGAASIAGPAAAQTYAPGYPVCLHVYGPATYYEC
                     RYNSLAQCNASASGRSAQCVTNPYVADAYEEPLGPRYRHRRVY"
     gene            complement(10578..11276)
                     /locus_tag="VN968_15670"
     CDS             complement(10578..11276)
                     /locus_tag="VN968_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30708.1"
                     /translation="MEQGEEDVPVVYLHEIKRQYTQGETTLTILNGAKLALWAGQSVA
                     LVAPSGAGKSTLLHIAGLLETPDDGEVYIGGTPTSGLSDIERTQIRRTDIGFVYQSHR
                     LLPEFSALENVMLPQMISGLKRSETVKRATEILSYLGLGDRIKHRPAELSGGEQQRVA
                     IARAVANAPRVLFADEPTGNLDPHTADHVFQALTQLVKATRVAMLIVTHNMDLAGRMD
                     RRVSLVDGQVVELT"
     gene            complement(11284..12564)
                     /locus_tag="VN968_15675"
     CDS             complement(11284..12564)
                     /locus_tag="VN968_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HXL30709.1"
                     /translation="MDETMSEPVRTPPFAPFEWLLSGRYLRARRKEGFISVIAGFSFL
                     GIMLGVATLIIVMAVMNGFRKELLDKILGLNGHLLVQPLESPLTDWKDVSERISQVAG
                     IRLAAPVVDGMGLANSPFNASGVLIRGIRADDLNNLTSIAKNIKQGTLEGFDEGQGVA
                     IGRRLADQLSLHAGDSVTLVSPKGAVTPMGTTPRIKPYKVAAVFEIGMTEYDGSFVFM
                     PLAEAQAYFNRPNDVTAIEVFTTNPDKIDGFRKTVTEAAGRPVFLVDWRQRNSTFFNA
                     LQVERNVMFLILTMIVLVAALNIVSGLIMLVKDKGSDIAILRTMGASQGSIMRVFLIT
                     GASIGVVGTLTGLFVGMLICLNIESIRQFLSWLTSTELFSPELYFLSKLPAEIDFGET
                     SAVVIMALTLSFLATLYPSWRAARLDPVDALRYE"
     gene            complement(12641..13960)
                     /locus_tag="VN968_15680"
     CDS             complement(12641..13960)
                     /locus_tag="VN968_15680"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HXL30710.1"
                     /translation="MRLSRFFLPILKENPKEAEIVSHRLMLRAGMMRQEAAGIYAWLP
                     LGLRVLKKIEQIVREEQDRAGAIELLMPTLQLADLWRESGRYDAYGPEMLRITDRHKR
                     ELLFGPTNEEMITEIFRAYVKSYKGLPLNLYHIQWKFRDEQRPRFGVMRGREFLMKDA
                     YSFDIDEAAARRSYNRMFIAYLRTFARMGLKAIPMRAETGPIGGDLSHEFIVLAETGE
                     SAVYCDSDVLDLPVPGEEIDYDGDLSPIIEQWTSVYAATEDVHDADRFDREVPAEKKV
                     RTRGIEVGQIFYFGTKYSDKMKAMVAGADGAEVAVHGGSYGVGVSRLVGAIIEACHDD
                     AGIKWPEAVAPFRAAILNLKQGAEDTDAACERLYRELTAKGVDVLYDDTDQRAGAKFA
                     AADLIGIPWQILVGPKGLAEGKLELKRRSDGSRENLSPAEVLARLSA"
     gene            complement(14039..14188)
                     /locus_tag="VN968_15685"
     CDS             complement(14039..14188)
                     /locus_tag="VN968_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30711.1"
                     /translation="MTDFEKIALKATSTFRRAEGGAIVLAPALIARLRSPEKRQEIRC
                     LSGFG"
     gene            complement(14378..14914)
                     /locus_tag="VN968_15690"
     CDS             complement(14378..14914)
                     /locus_tag="VN968_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HXL30712.1"
                     /translation="MQIERLGFISMACAAFLAALFAAPARAEEFQLGDLVISQAWSRA
                     TPGGAKIGAGYLTIENNGSVADRLMGGSGDVAAKIEVHEMAMSNGVMTMRPLDKGLAI
                     EPGKTVKLAPGGYHLMLIDLKNPLKQGDKVPLMLEFEKAGNVKLSLEVMGVGAQGPAG
                     ADNSDGQTHVKKRSGAKM"
     gene            complement(14998..15957)
                     /gene="copD"
                     /locus_tag="VN968_15695"
     CDS             complement(14998..15957)
                     /gene="copD"
                     /locus_tag="VN968_15695"
                     /inference="COORDINATES: protein motif:HMM:NF033808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HXL30713.1"
                     /translation="MNWLGAGIDGPLVVVRAIHFATTATITGSLTFRTLVAEAVSASA
                     RPAAIIVRTQTLRVAWICLAISTASGMIWLLLAAASMSGLPPGQSMTPDVLATVVNET
                     QFGFVAQIRFALAVILAGCLAYDRFALARGLALAMSLGLIAALAWTGHAGSTAGEIGV
                     LHLAADIFHLFAAAIWMGGLVSLVLLLSAVGRDQIQAGLPFAGDATRRFSAMGIVIVV
                     VVFATGIVNAWILVGSWHALIVTGYGRLLILKLALFAIMLLIAAANRFWLAPRLALPS
                     VNEPQLMVLRRLACNSMIEIALALMIFAVVGLLGTLHPAIHFL"
     gene            complement(15975..16334)
                     /locus_tag="VN968_15700"
     CDS             complement(15975..16334)
                     /locus_tag="VN968_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HXL30714.1"
                     /translation="MRRSSFFAGLMLPLLLSPTGSARAHAFLDHAEPRVGNKVAAAPR
                     QVTLWFTQKLEPAFSTISVTNSAGERVDTGKTRVSGEQMSISLRSGGAGTYHVTWRVL
                     SLDTHTTDGNFTFQVGP"
     gene            complement(16337..17038)
                     /locus_tag="VN968_15705"
     CDS             complement(16337..17038)
                     /locus_tag="VN968_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30715.1"
                     /translation="MGANGFQNLETTFKYRVFKDPEHEFVMSVGLGVEWGGTGAARVG
                     ADPFNTYTPTLYFGKGLGDLPDTLSWIRPVAITGQVGYAIPGERSTTTFGIDPDSGDP
                     AIDTKFHPRVLNWGGTIQYSMPYLKSAVYDLGLPDFINRFIPLVEATLQTPVSNTFTS
                     GTLTTGTSIRDFSGVGNTFQFGIEALIPINRQSGTSVGLIAQLHFYLDDIDPRGIGRP
                     IFGNAPLLATPFGGN"
     gene            complement(17400..17783)
                     /locus_tag="VN968_15710"
     CDS             complement(17400..17783)
                     /locus_tag="VN968_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 domain-containing protein"
                     /protein_id="HXL30716.1"
                     /translation="MRRRLRKFLPIVLVALAVQIFAPIGACWAASIAASDPLQTAVIC
                     HADTASAQGHTGQSDTTGQPRAHDGCCSVCSVLHTGAPVDTPQTVVTTPSHQPEQVVW
                     FDSVADLVGSRTGSHAQARAPPQLT"
     gene            18379..18798
                     /locus_tag="VN968_15715"
     CDS             18379..18798
                     /locus_tag="VN968_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HXL30717.1"
                     /translation="MMANTNLIQHSFELAAERCEDLTPLVYRRLFREHPEAEAMFRHE
                     ENDLVKGSMLALTIDAMLDLAGDRNGNFRMISCEVQSHDAYGTPRELFGKFFGVIADT
                     MREILRTEWSPEIEDAWRKLLGEIDEFIAQSEVPVDG"
     gene            19068..20090
                     /locus_tag="VN968_15720"
     CDS             19068..20090
                     /locus_tag="VN968_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HXL30718.1"
                     /translation="MSANDKSLKTRRDLLQTAATAGAATALLGGLGVSPALAAEVGRS
                     EKPLKAAFSNAGLQATWCAQGKQAAEYWGKLFNVEVTWFDGQLDAVKQRAAIDNMASQ
                     KWDFVAIQAFGIGTLTQPVQKMIDAGTPVIDMDTLIAPLDQINVHSFLAPDNEFMGAS
                     VTQALVGAIGGKGKVIMTQGALGHTGAQGRAKGFNSVVKQYPNIEVLDTQPADWDVSK
                     TARLWETYLTKYPQIDAAFFHNDDMALAAYNIMKAHNRTNILIGGVDAMPPAIQAVSE
                     GRMFATVRNPSCRIHGGAIIAGIAAVTAGEKSGQGIPKSVVTDGPVVTKANAAGMQWM
                     EDHFLI"
     gene            20098..21600
                     /locus_tag="VN968_15725"
     CDS             20098..21600
                     /locus_tag="VN968_15725"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HXL30719.1"
                     /translation="MPQGRSPILELHQITKAFGGVEALRGVDFALSAGEIHGLVGENG
                     AGKSTLMKIIAGVHAEFSGRFVLDGRETRFRSVRDARAAGIAMVHQELSVAPDLSVAE
                     NVFLGAQPTNRFGVMQWRRMAREAGEQLARFGIDVDPLSRLGDLPIGLQQLIEIARVL
                     SSGARIIILDEPTSALSPPEVERLFATLRQLRDEGTSIVFISHFIEDILHVSDTVTVF
                     RNGRKVIEAQAAATSKAALIEAMIGKGHEALEESYTDDITLPPPSDRPVVLKASRLSL
                     ARSLQDVTFDVRSGEVLGIYGFMGCGQLELARILFGKLGPDQGTLVVAGGEKAFRSTA
                     DARRAGIAFVPESRRAMLFHQEPVYKNISISILDRISRLFLKPSRERSIAMRRVQQLQ
                     IRPAAVELELGMLSGGNQQKVALAKWLSYPPRLLVLCEPTRGMDVGAKNDVINIIRDL
                     RAQGLAIIVLSTEPETILSLADRIIVLKRGAVVREFSNEAISKDRLMEAA"
     gene            21611..22642
                     /locus_tag="VN968_15730"
     CDS             21611..22642
                     /locus_tag="VN968_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL30720.1"
                     /translation="MVTGESVTAADRKRPRKIAAMLRSQMRNVAPFLTLICLSAFFAA
                     ASPSFATLDNLGNILTQVSVTGIIAVGLTFVILCAEIDLSIASIANVTGIAVAYFTAQ
                     ESYVNIANIPMPGWAAILLALALCAMLGLVNALGLTVIGIPSFIMTLAMMQIAAGISA
                     LLVRGQIAYKVPDLVTILGSSSIGGVPWIVIVAALMLLGGHLVLTYTRFGRYIYMVGG
                     NREAAEYSGLNVKLILGSVMVISAICSGIGGMLGVAHFGSAQQNEFDTYLLDSIAAVV
                     VGGTSLFGGRGGIGNTIVGLFVLGVLNNGLDHVNIDSFLKILIRGVILLAALVINVYA
                     QRWRDRAVE"
     gene            complement(22670..23017)
                     /locus_tag="VN968_15735"
     CDS             complement(22670..23017)
                     /locus_tag="VN968_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30721.1"
                     /translation="MIFQRTIVGLLVLAIAGLAASFYIEWKMSVQLRTIKAFVEGYVL
                     AEAVAACEKVQSTTPFKWNTGTHTAVIGLNSVKLDKNTTIASYTLVADSVYCDYDPIK
                     KKADIGSNFLERD"
     gene            complement(23048..23323)
                     /locus_tag="VN968_15740"
     CDS             complement(23048..23323)
                     /locus_tag="VN968_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HXL30722.1"
                     /translation="MVYTISTGFAIYFVLWWIVLFVTLPFGIRSQHEDGGGAAGTDPG
                     APVASQMGRKLIWTTVISAVIFAAAMLAYNAGYLNVERLSKLMGIPL"
     gene            complement(23333..>23697)
                     /gene="mce"
                     /locus_tag="VN968_15745"
     CDS             complement(23333..>23697)
                     /gene="mce"
                     /locus_tag="VN968_15745"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HXL30723.1"
                     /translation="AEQAARIYGAAFGADISAAVPLPEHGVITVFVTLPNTKIEFIQP
                     LGEASPIAKFLERNADGGIHHICYDVPDIIAARDILIKEGARVLGDGQPRIGAHGKPV
                     LFLHPKDFSGALVEIEQA"
BASE COUNT         4611 a         7397 c         7266 g         4423 t
ORIGIN      
        1 ccaatcgacg tctgccgtcc ggcgtcagcc accagcgtgc gtatctgcag gagccgcggg
       61 ggcgccgtgg cataaatggc ctgtgcggcc gacgatgcat ccggtgacgc ccggacagca
      121 ccgccgtcca tcgccacgat cgccagcaac agccccagcg cgcgcgcgac attggcgacc
      181 gaaatagcca agaaatcccc caatccgatc aacgccagac gtttagtccg aagttttcag
      241 aagccgccgt ttgcggcctc tcgtttcgac ctgctgtccg acgaacgcta ttccgccagt
      301 tgaagctccg cgagcgttgc cctcgccccc ggttcatcca agggaaaaaa gtcgaccggt
      361 ccaggattct cgctgccaga agtgacggct gaggcaacga gcatgccggc cagaccacct
      421 gcgactgtca tcccggtaag ggtactgtgc ctctcgctga cccttgcgag gaaaaaatac
      481 gtgcggccgg attccattgt gatgtcgcgc ttgctgtcgc ctggaaacat tgtctcggcg
      541 gccaggagct ggtgatgacc tgccggacga tcggcataaa gataggtccc aggctttagc
      601 cggccgatcg ggtcaccgtc gagcttcatg tcacaggcgc agctcccata gccaaggcca
      661 ctggcttttt cctgaagcaa gaccatgcgc gcttgccctg ctctcggcga gccgaccttc
      721 tgtatcatcg tcgcgtaatc agcaccgatt cttccgctcg cacagcccaa caacaaggcc
      781 gccgcaagcg acaacactac cccacgccaa tacatcacgc cccccattcc ccactggggt
      841 acctaaccaa aagcaccata cggttgcaac agcatcggca gctttcggcc gcccaattag
      901 cgcgcaagtc tctgtgctga cgaacgggtg ccatgcgcgt acgaatggcc gctggtagtg
      961 gccctgccca ggaaattcgc cggccgtctc gctgtggcca aaagtctaaa aggggtccgg
     1021 tcaaacctca gattgaggtg tgaccggttt cgctttcagc atcttggaac attcggcggg
     1081 catttcttgg ccgccgccgg cgcagccgcc cgtggctgcg gcggagctgg tcgcggcggg
     1141 ggcggcgccg gacgggccat ctggggcgcc ggtcgcggtg gcggcggggc cggtcgaggg
     1201 ggcggcggcg cggccattcg gggcggcggt ggcgctggcc ttgccatctg cggggccggg
     1261 cgcggcggtg gtgcggcccg atgtggcggc tcggcccgtt gcatttgagg cctcggcgcg
     1321 gccggcggcg cgggtcttgg aggtgccgca accggtttgg gtggcggcgg tggcggcgga
     1381 gccgcgtttg gccttcgcac cgtttccgga acgaccggct tggcttccgg ggcttgagcc
     1441 gaaccgcccg ctttcggttg acccgcccga aatgctggct cattggcccg tggctgcggg
     1501 gcgagtttcg cgttggggtt tggcgcaacc ggagcgggca ccgtcgcgcc tgggggtgca
     1561 ggaggtgtcc ccttcgcgcc cgggaccggc aaggcattgg ccctcggcaa cgcttgtccc
     1621 tgaggcagcg cattggggga taggtttgcc ggcaatggcg tatttggcgc agctttctga
     1681 ccggatggcg tgccgggagt gcccaacctg gctgccggat taatcgaggc gctcggcggc
     1741 accgccagtt tgccttgctg gatcagcgag gccctttgcg cgactgcggg cggcagcgcc
     1801 ggaccggtac cggactgagt gacaccgccc tgccccggtg cgcccttcac gccgggcgaa
     1861 gccgcaacac ccttggcgtt gggttggacg ttgccggccc ccggctgagg gggccggttg
     1921 atgacgttgt tgatgacggt cgtgttgtgg atgttggcga aaatgatgtt gttcggcggc
     1981 ggcaggacat aggccggctg ctggatgtag accgggatcg gcacaaagaa cggttgcggt
     2041 aggataaaca gcccgatcgg cggcggcggc ggctccaaca cgacgaaatc cgctggcggc
     2101 gccggcagga agaacagtgg cggcggcggt ggcggcgcaa agtcgaactc cggatcgctg
     2161 aacatcagca tggggcggtc gacgtagacg atttcctcgg gcggaggcgg cggcacgtcg
     2221 tagtcgatga ccgcgaacga cggcggcggc tccagcgaag cgatgagaat ggcaagtcga
     2281 cgacgagcat cgccggcatg cggaccgcgc ggatagcgtc tcagatacga ccagtaagct
     2341 tgcggcgtgt cggcccggta agagcggagc caggtaatgg cttcgcgccg cgcggcggca
     2401 attgcccgca cccgtttggc gagcggatcg ttgggatagg cggtcaggaa atcctcatag
     2461 ccttgcatgg tgtcgcgttc gagtgatgcg gcataggcat cctgcacgcc gaggtcgcgg
     2521 atcggcttgc cacggaccgc ggcaacctga tccggcgctg ccggcgcggg cgcatccggc
     2581 gcacgattga aaaacatgaa ctgcccatcg atcttttcag cgtcccatgg gacttgcgca
     2641 cccttgctcg tttcattgac gcgcagccgc acgcgattga agacttccgg cagcgacaat
     2701 ccgccggtgc ggatcatctc tgcgagcgac tgcgcgtaaa cgccatacgg accctgttcg
     2761 gcgggggcga tcgtgcccgg cgcggcgttg aacgcgatca gcatatgcgg atccggctcg
     2821 accagcgcga ggccactcgc aagcggctgg ccgccctcaa cgaagggctg ctgccgggcc
     2881 gcgtccagca ccacgacgcc ggccttcagc ggaagcgatg caagctggcg gacgtagtcg
     2941 ttgatccgca ttccctccgt tggaatgtcg gtatcgcgac tgatcgggga gtcgacgggt
     3001 atgaaataat tctcgcccgc cagctgcatt ccgtagcccg cgagatacac catcgcaacc
     3061 gtgtccggac ctgaggcttc ggccttctgg atgaagtcgc ggaagcttcg gcgcagcgtg
     3121 tcgccgtcga ggtcgcgggc cccgaccacg tcgaagcccg ccgcctgcaa ggtttgcgcg
     3181 atcagccctg cgtcgttggc cgcggtcgcg agcggcgact tggcgtaagc gccgtcgccg
     3241 acgaccagcg caatccgctt ttcctgctgc tgcgcggcgg cggaactcag gatccccggg
     3301 aacccgcaga gcaggatcag gcaaaacgaa agaatcgaaa aatttcttgt cacacgcatg
     3361 gctgccctgc ctgctcacgg ttcaactagg tatccgcgca tggagcacgc ggccagctga
     3421 acatcgcttg aacgaaacgc gccgccaatc caataaggca ttgcgcatga cacgcaagcg
     3481 gcgaagcagg cctatcggca gccgcctcaa gatgaacaag cttgatggta ttctcttgat
     3541 gccgcatgcc gcgacggatc ctgtagagct cgaatcccgg ttgacggccg cgataaccag
     3601 ttcggtcgat tggatcttgt ggaggagcag cgatggcgac gtgcacaata ggtgatctca
     3661 gccgccgtga gatcttgaag atgtccgcca taacgggcgt cgccagtgtg gtctcaacca
     3721 ggtttgcgat ggcgcgaacc gtcgtgccac gtacgccgga gcaaatactc ggtcccttct
     3781 atccactgaa accccttgat caaaatgccg atctgacgag ggtatccggt cgaccagggc
     3841 gcgccgaagg ccaggtcctc attgtgacgg gacgcgtgct caatctgaac ggggagcccg
     3901 ttcgcaatgc gaagattgaa atatggcaag ccaacgccca tgggcgctac acgcatccaa
     3961 gcgataccaa ccccgcgccg ctcgatccga actttgaggg ggcagccgtt ctgaccgctg
     4021 acgacgacgg tcgatatcgg ttcgagacga tcaaacccgc cgcgtatccc gtgggccctc
     4081 acttgatgcg accagcgcac attcacttcc aggtttccgg ccggcaggac aggctcgtga
     4141 cccagatgta cttcgaaggc gacccctaca acgccacgga ccgcattctg caaactgccg
     4201 gaagaaccga attgctgatc gccaagttgt tgccgccgac gcctgagatg gagccgaagt
     4261 cgaaattggc ggtgttcgac atcgtgctgt atgctggcta agcgcgtttt ccagcgaaag
     4321 cctgccccgg acttgacccg gggtggagac cggttcgcgt caagaaaacg cgtcagatca
     4381 agagtctaga gagccctgtt tcgattccat cgaaacggaa aaggctctgg tcggtacgca
     4441 cgctcgcccc gcctacagct gctgcacgtc caccaccccc gacaccgcct tgatggcgcc
     4501 ggcgatctgc ggggagaccc tgaaacggcc cgggagcttc atttcgacct cggtctcgag
     4561 gtcgagcatc atcaccagcg atacatcgcc attggccccg cccgccgcgt cgggaagcgg
     4621 ttttgtctga ggaccgcgga ccgggccgcc ctgtggcgcg ggctccggca tctgcagccg
     4681 cttggcgatg gactcgagcg gcctggtatc gcggacgaat atccgaagac ctttttgggt
     4741 tttcgcggcg gcggcatcca gcggctcggc gtgcagcacc cgcgcacgga catcctcgcc
     4801 ctgcagctcg gcgccgagtt gcagcagcac cgcggcaccc ggctccagca cttcccgata
     4861 ctgtgcgagc ccttcggaaa acagcacggc ttcgaaatgg ccggtcggat ctgacagccc
     4921 catgatgccc atcttgttgc cggtcttggt gcggcgttcc atgcgcgaca ccacggtggc
     4981 tgcaaccttg cccgcggtcg cgccggtttt gaccgcgcgc gaaaattccg cccaggtctg
     5041 cacccgcaac cgtttcagga ccgtggcgta gtcatcgagc ggatggccgg agaggaagaa
     5101 accgatcgcg tcatattcgc gccgcagccg ctccgccggc agccacggct cgacctgcgg
     5161 cagcatgatg gtcggcgcgt cggccgcacc gccgaacatg tcgttctgcc cgacggtcgc
     5221 cgcctcatgg ctgcgctggc acgctgcgag gatcgcttcg gcgccggcga acacccgtgc
     5281 ccgattggaa tccagcgcgt cgaacgcgcc ggccgcggcc aggctctcga tgacgcgctt
     5341 gttgatcgcg cgcggattca cgcgcgctgc gaaatcggcc aatgaggtga acaggccatc
     5401 ctttcgtgcg tcgacgatca gctcgaccgc ctgcccgccg acgcccttca ggccggcgag
     5461 cgcataatag atggtgcctt cgctgacctc gaaggtggcg ccggaacgat tgaccgaggg
     5521 cgcctcgacc ttgatgccca gtcgttgcgc ttcggcgcga aactcgctga gcttgtcggt
     5581 attgctgaga tcgagcgtca tcgacgccgc caggaactcg accgggtaat gcgccttcat
     5641 gtaggcggtg tgatacgaca ccagcgcata ggcggcggca tggctcttgt tgaagccgta
     5701 gtcggcaaac ttcgcgagca gttcaaagat cgtatccgcc tgccctttcg gtacgttgtt
     5761 cttcatcgcg ccggcaacga agccggcgcg ctgcttctcc atttccgaac ggatcttctt
     5821 gcccatcgcg cgacgcaaca ggtcggcctc gccgagcgaa tagccggcca tcacttgcgc
     5881 gatctgcatc acctgctcct ggtagatgat gacgccaaaa gtctccttca ggatcggctc
     5941 gagcatcggg tgcagatatt ccggctcttc gtcgccatgt ttgcgggcgc aataggtcgg
     6001 aatgttcgcc atcggacccg gacgatacag cgccaccagc gcgatgatat cctcgaagcg
     6061 gtcgggccgc atgtcgacca gcgcccgccg catgccctga ctttcaacct ggaacacgcc
     6121 gaccacatcg ccgcgcgcca gcatctggta actggcggca tcgtcgagtg gcagggtcgc
     6181 gagatcgaca tcgaccccgc gcttcctgag cagcttcacg gcgacatcga gcaccgtcag
     6241 cgtcttgagg ccaaggaagt cgaatttgac gaggcccgcg ggctcgaccc atttcatgtt
     6301 gaactgggtc accggcatgt cggatttcgg atcgcggtag agcgccacca gttcgctgag
     6361 cggacggtcg gcgatcacaa tgccggcggc atgggtggac gcgtggcggg tcaggccctc
     6421 gaggcgctgg gcgatgtcga aggcccgcgc caccaccggg tcttcatcgc gaaaggcctg
     6481 cagcttcggc tcgcttgcga tggcggcagc caacgtcacc ggcgcggccg gattttgcgg
     6541 caccagtttt gtgagtttgt cgacctgccc gtaaggcatc tgcaagacgc ggccgacgtc
     6601 gcgcagcacg ccccgcgcct gcagcgttcc gaaggtgatg atctgggcga cctgatcgcg
     6661 gccatagcgc tgctgcacgt aatcgatcac ctcgccgcgc cggtcctggc agaaatcgat
     6721 gtcgaagtcg ggcatcgaca cgcgctcggg attgagaaag cgctcgaaca gcaggccgaa
     6781 ccggatcggg tcgagatcgg tgatggtcag cgcccaggcg accaacgagc ccgcgccgga
     6841 gccgcggccc ggcccgaccg gaatgccctc ggcctttgcc cactggataa aatccgcgac
     6901 gatcagaaaa tagcccgcat agttcatgcg ggtgatgaca tcgatctcga aggcgagccg
     6961 ggcgcgatag tcctcctcgg agccgccttg cgagaggccg tgaaccctga gccggtcgtt
     7021 caggccttgc tcggcctgac ggcgcagttc cgccgcctcg tcgctttccg cctccaccgc
     7081 gttggtgccc gcgccgaccg tgaagcgcgg caggatcggc ttgcgcgtga cggggcggaa
     7141 cgagcagcgc tcggcaatct cgatcgtgga tgccagcgcc tccggcacgt cggcgaacag
     7201 caccgccatt tcggcgcggg tcttgaagcg gtgatccggg gtcaattgat cgcgatcggt
     7261 ctcggcgacg agacgcccgc cggcgataca gagcagcgcg tcgtgggctt cgtagtcgtc
     7321 ggcggttgcg aaatacggct cgttggttgc gaccagcggc aatcccttgg cataggccag
     7381 atcgatcagg ccagcttcgg cgcggcgttc tttctcgatg ccgtggcgct gcaattcgat
     7441 atagagccgg tcgccgaaca ggttcgccat ccggtcgcag cgcgtcgccg ccagcgccgc
     7501 ctgatcggca ttgatcgcca gcgcgatcgg cccctccgga ccaccggtga gcgcaatcag
     7561 gtcctcggcg tcgccctgca gccattcgaa cttgatgtgg ggcgactgat ggaccggcgt
     7621 ctccaaaaac gcccgcgagt tcagccgcat caggctgcga tagccgcgct cgcgcgcggc
     7681 cagcagcacg atccgcgccg gtgccgccgc cagcgcgttg cgggcgctcg ggtcctgatc
     7741 gccgaaatcg accgccagct cacagccgat gatgggctgg atgccgtagc cggccatctt
     7801 gtcggaaaat tccagcgccc cgaacatgtt gtcggtgtcg gtcagcgcca gcgccggctg
     7861 gcggtccgcc ttggccagtt cgccgagctt ctggatcttg atcgatccct tcagcagcga
     7921 ataggccgaa tggacgtgga gatggacaaa tccggcactg gacatggtcg ccgaaggttt
     7981 tgggatcatg gattggagcg atcactgggg cttgagggag cgcgggcggc ctcgaaggcc
     8041 gactcgctcc tcaatggtga tgcctggagc ctgaagtgtc cacgcggaac gcgcgattcc
     8101 agatcgccgc gccgcttttc cccaggtggg gcgtggggcc acgccgcccc atccccgtcc
     8161 caacgccggc cgcggcctca aagctgcgga atcaactgcg cccagatcgc aatcatggcg
     8221 acaaaaagcg cgatcgacgc cagtgccgca gcctcttcca cgaaagtctt caacatggct
     8281 tcctcctgat actggaacat aaggagaaca ttgttcctct tttgttccaa ggagtcaaga
     8341 gggtccactg ggtcaagacg gtagcgggtc agcttgaaga gtgaccccct gcggtcaatt
     8401 cttgaccggc ccaatcccca cgatccgccg aaccaaggaa gacgtgatat ggaaaccacc
     8461 gcccctatca actccaggat tctcccgggg tggcgggaga cgtgcgcatg gctgtccctc
     8521 gggatatcct catcaatctt ttcgctggtt ttctcgccga tttttcgagt cctggcggca
     8581 tgatcgcgat atcgatcagc ctcggaccga cgagcatgtg atcgtgagct ggcgccgcag
     8641 acgtatcgct tcggggaagc ctggcgtgga ttcttggcac ttcatcgcgc ttgccgtcgc
     8701 ggcaatcgca atcggggctg ccacttacgg cattgggtgg cgtagctcgc atccgtcagg
     8761 ggaagcaaaa tcccgcccgg cactgctgat tgaaaatggc agcgttcagc gggattccaa
     8821 atactttttg gctttgtttg ggacctatgc gcggagtggt aaaattgctc gtttctacgt
     8881 cagcatacaa actgacccag ggggcaatat tcagaccgca gaattttcaa aacctacact
     8941 ggtgaaggtt ctcgaagtta aggattttga aaaaggcgag ccggtccacg tcgccgtggc
     9001 atctttgcgc caggaggata gccctcaagg cccgtttagg ttggggtcct ccgacacagg
     9061 acttccctcc gaggccggat tcactaccac cagccttgtc aaggcgactg tgatcgtcaa
     9121 agacgacagc gatgccgaac agacttttgg gtttgtattg attccaagga ccaccacgat
     9181 ggaactgatg cgagccaatc gtagtaatct ggatcggcgc gccgaagatc ggcaacctgt
     9241 tcctgtattg aacggcgata acctgaagtt gatgaccgag acggatggag atagacaata
     9301 gcgatcgtct ctcacttctg atcaatcagc caaccctgcg atttctcctt gatccgttgc
     9361 agcgcgtcag ctccgctggc cgcatatccc tggctgcccg gctttcgact tgttcttgat
     9421 caatgcttcg tatccaaggt caaagtttac gacaacggac cggcgagttc ggaagcgccg
     9481 gcacccgtga tcgtgagttg acggaaaccc gcttgcgcgg cttgggttgg tccgaaggaa
     9541 tcaatgcaca atcggccgcg gccgaacgga ggctttcatg cgtaatttag ttctggcttt
     9601 gctggcggcg ggcggcttgg ccatgatcgg cgccgcgccg gcgcaggccg ttggcacccg
     9661 ctatccgttc tgcattcagg gccaggacaa tccgggactg agcaactgca ccttcacgag
     9721 ctacgaacaa tgtcaggctt ccgcatcggg gcggtttctc tattgtgtgg aaaatcccta
     9781 ttacaatccc ggcgagcccg atccgcgcgc ctatcgcagc cgccaccggg tacggtccgt
     9841 ctacccgtct tactgatcgc gcggcgagtt gaaactccgc tgcgcacctc acgcgcgaac
     9901 gtgaattgac ggggaccacc cggtcctgtc tgttcgcgag cgggagaaaa tcgttcgaag
     9961 ggcacggagg acgccatgcg cgaggtgttt ggggcgctga tactgtgttg tgccggctcg
    10021 acgttgggcg cgctgccggc cgcggcgcgg gattacccgt tctgcatcaa gggctgcgat
    10081 ttcggctcag gccgtggcga ctgcagtttt tcgagctacc agcagtgtca ggcgaccgcc
    10141 tccgggctca ccgccacttg cgcggaaaat ccctacttta acgccaaagc ggagtggcag
    10201 cctgatcgcg gccgccaatc cccaaggaga tactgatgcg cagtctgttt ttcgcgacca
    10261 tggcgattgg ggcggcctcg atagccggac cggcggcggc ccaaacatac gcgcctggct
    10321 atccggtttg cctgcacgtg tatggaccgg ccacctacta cgaatgccgc tacaattcac
    10381 tggctcaatg caacgcctcg gcgtcgggcc gctcggccca gtgcgtgacc aatccctatg
    10441 tcgcagacgc gtacgaagag ccgctcggac cgcgctacag gcatcgccgc gtctactagg
    10501 cgcggcgccc acctcactac tcgaagcgca gcgagcccat tttggggtag ctcagctcca
    10561 ggccgctgac gcaaacccta cgtcagctcg acgacctggc cgtccaccag cgacacgcgg
    10621 cggtccatcc ggccggccag atccatgttg tgggttacga tcagcatcgc cacccgggtc
    10681 gccttgacca gttgggtcag ggcctggaac acatggtccg cggtgtgggg gtcgagattt
    10741 ccggtcggct cgtccgcaaa cagcacgcgc ggcgcgttgg cgaccgcacg cgcgatcgcg
    10801 acgcgctgct gctcgccgcc cgacagttcc gccggccggt gcttgatacg atcgccgagg
    10861 ccaagatagg agaggatttc ggtggcgcgt ttgacagtct cggagcgctt gaggccactg
    10921 atcatctgcg gcagcatgac attttcgagt gcggagaatt ccggcagcag ccggtgcgac
    10981 tgatagacaa agccgatgtc ggtgcggcga atctgggttc gctcgatgtc ggataatccg
    11041 gacgtaggcg tgccgccgat atacacctcg ccatcatcgg gggtctccaa caggcctgcg
    11101 atatgcaaca gcgtcgactt gccggcgccc gacggcgcca ccagcgcgac cgactgtccc
    11161 gcccacagcg caagcttggc gccgttgaga atggtcagcg tcgtttcccc ttgcgtgtac
    11221 tgacgtttta tctcgtggag atagacgacc ggtacatctt cctccccctg ctccatctcg
    11281 tcctcactcg tatcggagcg cgtcgacggg atcgaggcgg gcggcacgcc atgatggata
    11341 cagcgtcgcg aggaaggaca gcgtcagcgc catgatcacg accgcgctgg tctcgccgaa
    11401 atcgatttcg gccggcagct tggagagaaa ataaagctcc ggcgaaaaca attcggtgct
    11461 ggtgagccag gacagaaact gtcggatcga ctcgatattc aggcagatca gcataccgac
    11521 gaaaagcccg gtcagggtgc cgaccacacc gatcgaagcc cccgtgatca ggaacacccg
    11581 catgatcgat ccctgcgaag cgcccatggt gcgcaggatc gcaatatcgc tgcccttgtc
    11641 cttcaccagc atgatcaggc ccgagacgat attgagcgcc gcgaccagca cgatcatggt
    11701 gaggatcagg aacatcacgt tgcgctcgac ctggagcgca ttgaagaaag tcgagttgcg
    11761 ctgccgccag tccaccagga acaccggccg tccggcggcc tcggtcacgg tcttgcggaa
    11821 gccgtcgatc ttgtcgggat tggtggtgaa cacctcgatc gcggtcacgt cattggggcg
    11881 attgaaataa gcctgcgcct ccgcaagcgg catgaacacg aagctcccgt catattcggt
    11941 catgccgatc tcgaacacgg ccgcgacttt gtaaggtttg atacgcggcg tcgtgcccat
    12001 cggggttacc gccccctttg gcgagaccaa ggtgacgctg tctccggcgt gcagcgacag
    12061 ctggtcggca agtcggcggc cgatcgcgac gccctgccct tcgtcgaatc cctcgagcgt
    12121 gccttgttta atgttcttgg cgatcgaggt gaggttgttg agatcgtcgg cgcgaatgcc
    12181 gcgaatgagc acgccggagg cattgaacgg tgagtttgcc agtcccatgc cgtccaccac
    12241 aggcgcggcc agccggatgc ccgcgacctg gctgatgcgc tcggagacgt ccttccagtc
    12301 ggtcagcggc gattcgagcg gctgcaccaa aagatgcccg ttgaggccga gaatcttgtc
    12361 cagcaattcc ttgcgaaacc cgttcatgac ggccatcacg atgatcagcg tcgcaacgcc
    12421 gagcatgatg ccgaggaacg agaaaccggc gatgacggag atgaatcctt ccttgcgacg
    12481 tgcccgcaaa tagcgcccgg acaacagcca ttcgaatggc gcgaaaggtg gggttcgaac
    12541 tggctcgctc atggtttcat ccatagatcg attttcacac gattcgggct aattgtggcc
    12601 gcgattgccg gacattccgg cacgcgcagt ggataattcg tcatgcgctc agccgcgcca
    12661 gcacctcggc cggactcaaa ttctcgcgcg atccatcgct gcgccgctta agctcgagct
    12721 tgccttcggc gaggcccttc gggcccacca gaatctgcca gggaatgccg atcaggtcgg
    12781 cggcggcaaa cttggcgccg gcgcgctggt cggtgtcgtc gtacaagaca tcgacgccct
    12841 tggccgtgag ttcgcgataa agccgttcgc acgcggcgtc ggtgtcttcg gcgccctgct
    12901 tcaaattcag gatcgccgcc cggaacggcg ccaccgcttc cggccatttg atgccggcat
    12961 cgtcatggca ggcttcgatg atcgcgccga ccagccgcga gaccccgacg ccgtaggagc
    13021 cgccatggac cgcgacctcg gccccgtcgg cgcccgcaac catggccttc atcttgtctg
    13081 aatatttggt gccaaagtag aaaatctggc caacctcgat gccccttgtg cgcactttct
    13141 tctcggcggg aacttcgcgg tcgaagcggt cggcgtcgtg gacgtcttcg gtcgcggcgt
    13201 aaaccgaggt ccattgctcg atgatcggtg agagatcgcc gtcgtagtcg atctcctcgc
    13261 cgggcaccgg cagatcgagc acgtcgctgt cgcaatagac cgccgactcg ccggtctccg
    13321 cgagcacgat gaattcgtgg ctgaggtcgc cgccgatcgg gccggtctcc gcgcgcatcg
    13381 ggatcgcctt cagtcccatc cgcgcgaagg tacgcaagta cgcgatgaac atcctgttat
    13441 aggagcgccg agccgccgct tcgtcgatgt cgaatgaata agcatccttc atcaggaatt
    13501 cgcgaccgcg catgacgccg aaccggggac gctgctcgtc gcggaacttc cactggatat
    13561 gatagagatt gagcggcagg cctttgtacg atttcacgta agcgcggaaa atctcggtga
    13621 tcatttcctc gttggtcggg ccgaacagca attcgcgctt gtgccggtcg gtgatccgca
    13681 gcatctccgg accataggca tcgtaacggc cgctttcgcg ccagaggtca gccaattgca
    13741 gcgtcggcat caacaattcg atggcgccgg cacgatcctg ttcctcacga acgatctgct
    13801 cgatcttctt cagcacccgc aggcccagcg gcagccaggc ataaatgccg gcggcttcct
    13861 gccgcatcat gccagcacgc agcatcaggc gatgcgacac gatctcggcc tccttcggat
    13921 tctcctttag gatgggtaga aagaaccgcg ataatcgcat gggcatactc aaagaatcgg
    13981 tagaacacgg gagagccgca gtgaaaccgg atcgggcccg aaaacacaag ggcgcgcatc
    14041 aaccaaagcc gctaaggcag cggatttcct gccgtttttc cggtgatctt aaccgggcga
    14101 ttaaggcggg ggcgagaaca atcgcacccc cctcggcgcg ccggaacgtc gaagtcgcct
    14161 tcagcgcgat cttttcaaaa tcggtcacca ggcgtccgcg cccggcacgg ccttgcgctc
    14221 ccgcagcaag aagtcgccgc tgccgaccgg gccaaaccac gacctggaac ctcgccctgt
    14281 cggaatggat atggttgccc gtggcgcgcc gcagccagcc ggaacgtggc gcgcacggcc
    14341 ccgacgatga acagactacc cttcgacacc atcagcctca catcttcgcg ccggaacgct
    14401 ttttcacgtg cgtctggccg tccgaattat ccgcgccggc agggccttgc gcgcccacgc
    14461 ccatcacctc caacgagagc ttcacattgc cggccttttc gaactcaagc atgagcggca
    14521 ccttgtcgcc ctgcttgagt gggttcttga gatcgatcag catcagatga tatccgccgg
    14581 gcgcaagctt gacggttttg ccgggctcaa tagccaaacc tttgtcaagc ggacgcatcg
    14641 tcatcacgcc attgctcatg gccatctcat ggacctcgat cttggcggcg acatcgcccg
    14701 agccgcccat cagccgatcc gccacggagc cgttattctc gatggtcaga tagccggccc
    14761 cgattttggc gccgcccggc gtggcgcggc tccaggcttg cgagatcacg agatcgccga
    14821 gctggaactc ctcggcgcgc gcaggcgcgg cgaacagcgc tgcgaggaaa gcggcgcagg
    14881 ccattgatat gaaccctaat ctttcgattt gcattttggt gtccctcttt gatcgctttc
    14941 aacgaacagg ttgcatcggc gggctggaaa gatttgcggt ggtatgaatt cagcaattta
    15001 caaaaagtgg attgcgggat gcaacgtacc gagcagtccg acaacagcaa aaatcatcaa
    15061 cgccaaagcg atttcgatca tactgttaca cgcaagtcga cgaagcacca tgagctgcgg
    15121 ctcattgacc gacgggaggg caagccgcgg cgccagccaa aaccgattgg ccgcagcaat
    15181 caagagcatt atggcaaaca gcgccagctt caagatcagg agccttccgt aaccggtgac
    15241 gatcaacgcg tgccaggatc cgaccagaat ccaagcgttg acgatcccgg ttgcaaaaac
    15301 cacaacgaca atcacgatgc ccatcgccga aaagcgccgt gtggcgtccc ccgcaaatgg
    15361 caggcccgcc tggatctgat cgcgtcccac tgccgacaac agaagtacca gcgatacaag
    15421 acctcccatc caaattgcgg cggcaaagag gtggaatatg tcggcggcca gatgcaagac
    15481 tccgatttcg cccgctgtcg atcccgcgtg cccggtccag gccaaggccg cgatcaggcc
    15541 cagcgacatt gccagcgcca gcccccgcgc caacgcaaag cggtcgtacg ccagacaacc
    15601 ggccaaaatg accgccagcg cgaagcggat ttgcgcgacg aatccgaatt gtgtctcatt
    15661 caccaccgtc gccagcacat ccggagtcat ggactgtccc ggcgggagac cactcatcga
    15721 ggctgccgcc aacaacagcc aaatcatgcc ggatgctgtt gatatcgcga ggcagatcca
    15781 ggcaacgcga agggtttgcg tgcgaacaat gatcgcggcc ggtctcgccg aagccgagac
    15841 ggcctcggcg accaatgtcc ggaaggtcag acttcccgta atggtcgccg tggtagcaaa
    15901 gtgtattgcg cgaacgacga ccaggggacc gtcgattccc gctccgagcc agttcattgg
    15961 cttgtccctg ggctttacgg gccgacctga aaggtgaaat taccgtcggt cgtgtgcgta
    16021 tccaacgaga ggacgcgcca agtgacatgg tatgttccag cgccccctga gcgtagcgat
    16081 atcgacatct gttcaccgct gacacgggtt ttgccggtat cgacccgttc accggcggag
    16141 ttggtcacgc tgatggtgct gaaagccggt tccagttttt gagtgaacca aagcgtcacc
    16201 tggcgcggag cggcggcgac cttgttgccg acacgcggtt cggcatgatc gagaaaggca
    16261 tgcgctcggg cgctacccgt tggggacaag agcaacggaa gcatcaatcc ggcaaaaaaa
    16321 gatgaacggc gcatgatcaa ttccctccaa acggcgttgc gagtagcggg gcattgccaa
    16381 agatcggcct cccgatcccg cgcggatcga tatcgtcaag gtagaagtgc agttgcgcga
    16441 tcaggccgac actggtcccg ctctgccggt tgatcggaat gagggcttcg ataccgaact
    16501 gaaacgtatt tccgacccca gagaaatccc ggattgatgt gccggtagtc agggtcccgg
    16561 aagtaaaggt attcgagacc ggcgtctgca acgtcgcttc aaccagcgga atgaaccgat
    16621 tgataaaatc cggcaggccg agatcgtaaa ctgctgattt gagatagggc atgctgtatt
    16681 gaatcgtccc accccaattc aaaactcgcg gatggaattt cgtatcgatc gccggatcgc
    16741 cactgtcagg gtcgattccg aaggtggtgg tcgaacgctc gcccggaatc gcataaccaa
    16801 cctgtcccgt aatggcgacc ggtcgaatcc aggacagtgt gtcgggaaga tcacccaacc
    16861 ctttgccgaa atacagtgtc ggtgtgtagg tgttgaaggg gtccgcgccg acgcgtgccg
    16921 caccggtccc accccattcg acgccgagac cgaccgacat cacgaactca tgctctgggt
    16981 ccttgaagac ccggtatttg aatgttgtct ccagattttg aaatccgttg gctcccatac
    17041 cgctcggccc tccgggcggg ctgaagtggc tatagattga tccaaaagat accgcgaacg
    17101 cctcggtgat tcgcttggaa aactcgcccg aaatatcgca ttgcttaaag gaaggatcgt
    17161 cgccggtctt gaaggcggcg accgtcggta ttgaaagttc gtcgttgacg ccgggatcat
    17221 cgatgccaag cgtggccggg aagaagcggt tgccgacaat ttcgtgggca aagctttcac
    17281 cgaggggaac gacattattg cgagccctga caggcccgcc cgcaaacggc gcatcgacat
    17341 gaaaattctc cgcatctgtt gagtggtgaa cgcatcgaag cggccaacgg ccgccaagtt
    17401 cacgtcaact gcggtggcgc ccgggcctgg gcgtgagaac cggttcgaga accgaccagg
    17461 tcagcgacgc tgtcgaacca aaccacctgc tcgggctggt gactgggcgt cgtgacaacg
    17521 gtctgcggcg tgtcgacggg tgccccggtg tgaagcacac tgcaaaccga gcagcagccg
    17581 tcatgggcgc gaggctggcc ggttgtatcg ctctggccag tgtgtccctg cgcggaagcc
    17641 gtgtcggcgt gacaaataac ggccgtctgc agcggatcgg aggcggcgat gctggccgcc
    17701 cagcatgcgc cgatcggtgc gaagatctgc accgccaggg caaccaggac gatcggaaga
    17761 aatttcctca gccgccgccg catcgcgaat cccaccttcc ccaacactgc caattaatca
    17821 tgcaccgagc agggtgtcga gatcgccgtc cccgcgatgg gcgtcaggca cgatcatgcg
    17881 tttcaagagt aacgccctcg cttggtatgc caggacatca ccagaccccg acggtactgt
    17941 tgggcgcgcg cgctattttc aaagctcagc cgcgccactg gtaaccttgc ccggactccg
    18001 agttcagcac tcttacgcaa cccggggtgg cgaaattccg atcatttcgt tgactgctcg
    18061 aattttgaac aatcgttgct gaaaatgatg acaagacgaa aaagatggtc taacactgca
    18121 gcttcagact gaccgcaagg ttttaagtct ggtggtccaa gtctcgggag gagccctggc
    18181 gcgcacaagc agcgtcgccc cgtccatcaa gatcaaatcc aaatcatcgg ggaaataggg
    18241 tcgacacaat tttcagagca gggcttctcg ccctgctttt ttttcagagc agggcttctc
    18301 gcccagctct tttttcgaga tgctcttctg caagatttgg acaaatcgct gcactgtgcg
    18361 tttcaagacg aagcggaagt gatggcaaac accaatctga tccagcacag tttcgaactg
    18421 gccgcggagc gttgcgagga tttgacgccg ctggtctacc gccgcctgtt ccgcgaacac
    18481 cctgaagccg aagccatgtt ccggcacgag gagaacgacc tcgtcaaggg ttcaatgctg
    18541 gccctgacca tcgacgcgat gctggatctc gccggcgatc gcaacggcaa ctttcgcatg
    18601 atcagttgcg aggtgcagtc gcacgacgcc tatggcacgc cgcgcgaact gttcggaaaa
    18661 ttctttggcg tgatcgctga tacgatgcgc gagattctcc ggaccgaatg gtcgcccgag
    18721 attgaagatg cctggcggaa gctgctcggc gagatcgacg agttcatcgc gcaaagcgaa
    18781 gtaccggttg acggctgatt tccatcccgc ttccggaaat tcgatccgat gtccgccctc
    18841 ccctccacga gcccttaggc ggagcaagaa ctcaacggcg gatgtacggc cgaaatggtt
    18901 tcaccgacaa gcggttctcg ctcgttctgc gcgggacgag gcctgagcaa gtaccacagg
    18961 tcagagcagg tggcgtgaat gccataaatg tgagacactg cagtcttccg tggcgcaaca
    19021 taaacgacgc catcgggcaa caaatattaa aagcgaggga gcaagcaatg tccgcgaacg
    19081 acaaatctct aaagaccagg cgtgatcttc ttcagacggc ggcgaccgcc ggcgcggcga
    19141 cggccctgct cggcggatta ggtgtcagcc cagcgctggc ggcggaagtg ggacgttcgg
    19201 agaagccact gaaggccgcg ttctccaatg caggcctgca ggcaacatgg tgtgcgcagg
    19261 gcaagcaggc ggccgaatat tggggcaagc tgttcaacgt cgaggtcact tggtttgacg
    19321 gtcaactcga cgccgtcaaa cagcgcgccg ccatcgacaa catggcctca cagaaatggg
    19381 acttcgtcgc catccaggcg ttcggtatcg gcacgctgac ccagcccgtg cagaagatga
    19441 tcgatgccgg cacgcctgtc atcgacatgg acacgctgat cgcaccgctc gaccagatca
    19501 atgtccactc gtttctcgcg cccgacaatg aattcatggg ggcctcggtg acgcaggcgc
    19561 tggtcggcgc catcggcggc aagggcaagg tgatcatgac acagggcgcg ctcggccaca
    19621 ccggcgcaca aggccgcgcc aagggcttca actcggtcgt caagcagtat ccaaacatcg
    19681 aggtgctcga tacccagccg gcggactggg acgtctcgaa gacggcgcgg ctatgggaga
    19741 cctacctcac caaatacccg cagatcgacg cggccttctt ccacaatgac gacatggcac
    19801 ttgccgccta caacatcatg aaggcgcaca accgtaccaa catcctgatt ggcggggtcg
    19861 atgccatgcc gccggcgatc caggcggtga gcgaaggccg tatgttcgcc accgtccgca
    19921 acccatcgtg ccgtatccac ggcggcgcca ttatcgccgg catcgccgcc gtaaccgcgg
    19981 gagaaaagag cggacagggc atcccgaaga gcgtcgtgac ggacggtccg gtcgtgacca
    20041 aagccaacgc ggctggaatg caatggatgg aggatcattt cctgatctga gccctgtatg
    20101 ccgcaaggcc gctcgcccat cctcgaactg caccagatca cgaaggcctt cggcggcgtc
    20161 gaggcgctcc gtggggtcga cttcgcgctc tccgccggcg agatccatgg tctcgtcggc
    20221 gaaaacggcg cggggaagag cacgctgatg aagatcattg ccggtgttca tgcggaattc
    20281 tcgggccgtt tcgtgctcga tgggcgggag acccgttttc gatcggtccg cgacgcgcgc
    20341 gcggccggca tcgccatggt ccatcaggaa ctcagcgtcg cgcccgattt gtcggtggcc
    20401 gagaacgtgt ttctcggggc gcagccgacc aatcgcttcg gcgtcatgca gtggcggcgc
    20461 atggcgcgcg aagccggtga acaactcgcc cgattcggta tcgacgtcga tccgctttcc
    20521 cggctcggcg acctgccgat cggcttgcag cagttgatcg agatcgcccg cgttttgtcg
    20581 tccggcgcgc gcatcatcat cctcgatgag ccaacctcgg cgctctcgcc gccggaggtg
    20641 gagcgcctgt ttgcgacctt gcgacagctg cgggacgaag gcaccagcat cgtcttcatt
    20701 tcccatttca tcgaggacat cctgcacgtc tcggacacgg tgacggtttt ccgcaacggc
    20761 aggaaggtca tcgaagcgca ggcggccgcg accagcaagg ctgccctgat cgaagcgatg
    20821 atcggcaagg gccacgaggc gctcgaggaa agctataccg acgatatcac gctgccgccg
    20881 ccgagcgatc ggccggtggt gctgaaggca agccggctct cgctcgcccg cagcctccag
    20941 gacgtcacct tcgacgtacg ctccggcgaa gtccttggca tttacggctt catgggatgc
    21001 gggcaactcg aattggcacg gatcctcttc ggcaagctcg ggcccgacca aggtaccctg
    21061 gtcgtggctg gcggcgagaa ggccttccgc agcaccgccg atgcgcggcg ggccggcata
    21121 gcgttcgttc ccgagagccg ccgcgccatg ctcttccacc aggagccggt ctacaagaac
    21181 atctcgatca gcatcctcga tcgcatttca cgcctgtttc tcaagccgtc acgcgagcgg
    21241 tcgatcgcca tgaggcgagt ccagcagttg cagatcaggc cggcggccgt cgaactcgag
    21301 ctcggcatgc tgtccggcgg caaccagcag aaagtcgccc ttgcgaagtg gctgagctat
    21361 ccgccgcgtc ttctggtgct gtgcgagccg acgcgcggga tggacgttgg cgcgaagaac
    21421 gacgtgatca acatcatccg cgatctgcgc gcgcagggac ttgccattat cgtgctctcg
    21481 accgaacccg agacgattct ttcgctcgcc gatcgcatca tcgtcctcaa gcgcggcgcg
    21541 gtggtgcgcg aattcagcaa cgaggcgatc agcaaggacc gtctgatgga agcggcgtga
    21601 ggagaccgat atggtgacgg gtgaaagcgt aacagcggcg gatcgcaagc gaccgcgcaa
    21661 gatcgccgcc atgttgcgct cccagatgcg caatgtcgcg ccgttcctga cgctgatatg
    21721 cctcagtgcc ttcttcgccg ccgcgagtcc ttcctttgcg acgctcgaca acctcggcaa
    21781 tattctgacc caggtttcgg tcacgggcat catcgccgtc ggcctcacct tcgtgatcct
    21841 ctgcgccgag atcgacctgt cgatcgcgag catcgccaac gtcacgggca ttgcggtcgc
    21901 ttatttcacc gcacaggaat cctacgtgaa cattgccaac atcccgatgc ccgggtgggc
    21961 ggcgatcctg ctggcgctgg cgctatgcgc gatgctcggc ctcgtcaacg cgcttgggct
    22021 cacggtcatc ggcatccctt ccttcatcat gacgctcgcg atgatgcaga tcgcagccgg
    22081 catatcggca ctgctggtgc gcgggcagat tgcctacaag gtgcccgatc tcgtcacaat
    22141 actcggatca tcatcgatcg gcggcgtacc ctggatcgtg atcgtagccg cattgatgct
    22201 gctcggcggc catctggtcc tcacctacac acgctttggc cgctacattt acatggtcgg
    22261 cggcaatcgc gaggcggcgg aatattccgg actcaatgtc aaattgattc tcggcagtgt
    22321 catggtcatc tcggcgatct gctccggcat cggcggcatg ctgggagtcg cacatttcgg
    22381 aagcgcgcaa cagaacgagt tcgacaccta cctgctcgat tcgatcgccg ccgtcgtggt
    22441 cggcggcacg agcctgttcg gcggccgcgg cggcatcggc aataccattg tcggcctgtt
    22501 cgtgcttggc gtcctcaaca atggactcga ccacgtcaac atcgacagct tcctgaaaat
    22561 actgatccgt ggtgtcatcc tgctcgcggc gctcgtcatc aatgtctatg cgcagaggtg
    22621 gcgggaccgg gctgtcgaat aggtcgatac tgcagccaga cttacggatt caatcccgct
    22681 cgaggaaatt cgatccgatg tcggctttct tcttgatcgg atcgtaatcg caatagacgc
    22741 tgtcggcgac gagcgtgtag ctggcaatgg tcgtgttctt gtcgagcttc accgagttca
    22801 atccgatcac cgcggtatgg gtcccggtat tccatttgaa cggggttgtg ctctgtacct
    22861 tttcacaggc tgcaaccgct tccgcaagca cataaccttc aacgaaggct ttgatggtcc
    22921 ggagttggac agacatcttc cattcgatat agaacgacgc tgccaagccg gcgatcgcca
    22981 gaaccaaaag tccgacgatg gtcctttgaa aaatcattcg ctctcccagc ctgcaaaagt
    23041 agatcggtca gagcggaatc cccataagct tcgacagccg ctcgacattc agatagcccg
    23101 cattataggc cagcatcgcg gcagcgaaaa tgactgccga tatcaccgtg gtccagatca
    23161 gcttgcggcc catctggctc gcgaccggtg cgcccggatc ggtgcccgcc gctccgccgc
    23221 cgtcctcgtg ctggctgcgg atcccgaacg gcagcgtcac gaacagcacg atccaccaca
    23281 gcacgaagta gatcgcgaac ccggtggaga tggtgtagac catcgctaag cctcaagcct
    23341 gttcgatttc gacgagcgcg ccggaaaaat ccttcgggtg cagaaacagc accggcttgc
    23401 catgagcgcc gatcctgggc tgcccgtcgc cgagcacccg cgcgccctcc ttgatcagga
    23461 tgtcgcgcgc cgcgatgata tcgggtacgt cgtagcagat gtggtgaatg ccgccgtcgg
    23521 cgttgcgctc gagaaatttg gcgatcggcg aggcttcgcc gagcggctga atgaattcga
    23581 tcttggtgtt gggcagcgtt acgaacacgg taatcacgcc gtgctccggc aacggcaccg
    23641 ccgccgagat atcggcgcca aacgccgcgc catagatcct ggcggcctgt tcggcgt
//
LOCUS       DATNOI010000393         8576 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_301076,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000393 DATNOI010000000
VERSION     DATNOI010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301076"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(781..1236)
                     /locus_tag="VN968_15750"
     CDS             complement(781..1236)
                     /locus_tag="VN968_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30724.1"
                     /translation="MRLKPAFRFSIYAAVTVLFLIGASWLVADWQKNISSDEIWQQAA
                     ANLLMVHGGAAMVTLLLLGALIPVHMLRSWRSGNNLVSGSVMITLNTLLIMTAFGLYY
                     LGSEAVRPWMSWIHIVAGFCLPAWLPLHVLLGRRRSKATLKMPSEERSN"
     gene            complement(1226..2137)
                     /locus_tag="VN968_15755"
     CDS             complement(1226..2137)
                     /locus_tag="VN968_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HXL30725.1"
                     /translation="MATGSDSIRRARPLLGTFVEIGVTGEAAFAMEAAVDAAFEAVAN
                     VHRLMSFHAADSDVSRLNRQAGLRPTRVHAWTFEVLEAAVEMHRRSNGIFDIAVAPTL
                     QTMGLLPRSAGDPPIPSGPQLPDAIELFPGETVRFRSPDVRIDLGGIAKGFAVDRAVE
                     ALRRFDVQSGIVNAGGDLAVFGRDPQMIHIRDPRDPGRPICRVEITDGAIASTARRFD
                     PFRSSETTASAVVDPGTRKPTDTIDAATVRAPTCMVADALTKIVMISGTDATEVLEHY
                     NASALLISTDGDVHITLDWHHAVHLAA"
     gene            complement(2118..2591)
                     /locus_tag="VN968_15760"
     CDS             complement(2118..2591)
                     /locus_tag="VN968_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HXL30726.1"
                     /translation="MTKWVRYAAPAVAVASIASPAYAIQYLSIGEAQKQAFPSATQFT
                     EVQAGRVWKAEAGGRGIGFFVFDRVVGKHLFIDYAVALSTSGVVQKVEILQYRESYGG
                     EVNSPSWLSQFVGKASGSALRINSDIRNIAGATLSSTHLTEGVKRILAVYGNRLR"
     gene            complement(2665..4179)
                     /locus_tag="VN968_15765"
     CDS             complement(2665..4179)
                     /locus_tag="VN968_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30727.1"
                     /translation="MSIIGCSGAWAADGEANDALLKKLEKMEQRIQMLEGKLKQKQAA
                     AQAGPVAASPSTPPAAAPSNAQPTTSAQPTAPARAARAEIASTAPSPTPTAPANNGIV
                     GLADSPVPGLSIGAYGEIKFGSQQNPAANGQWQKGFDAHRIVLLPTYAITPNIIFNAE
                     IEFEHGGIAFDSDDKRHGSAEIEQIWVDFKVMDQFNWRSPGIDLVPIGYINQHHEPTQ
                     FYSVNRPQLYNGLIPSTWHVPSSSVYGRIADGLSYQIQVSSSVEDFGDDFGLRTDANA
                     VPPFPTGYAAGIDGLSALANSRPVVGDFRQLSNTLAYAGKLDFAPPFLPGFAGSVSAY
                     YTPNTTPRGAHDDLGNILGGSSLALFDAEFRYRVQGTGLELRGEYVQARFGHPENLRA
                     NNDSDPTNNVGKKMWGYSGEVAYHVPLGTIINSEWEAVPFYRYTRQDFQTAGFAGTDD
                     NTPTGAGQRQYHTAGVAVFPSPKVVLKATYQRVIDKSIVGALSDSFLGGVGFFF"
     gene            4172..4477
                     /locus_tag="VN968_15770"
     CDS             4172..4477
                     /locus_tag="VN968_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30728.1"
                     /translation="MDIAVAASSALRLEQRTGWIETGKRENWCNDMGPPKSAEIKVSA
                     TSGDLTPLQNISGIGNGLLDWNLSILELEQNQSDPTFADRGRLKRTVPYVPNAVNIF"
     gene            complement(4743..5654)
                     /locus_tag="VN968_15775"
     CDS             complement(4743..5654)
                     /locus_tag="VN968_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HXL30729.1"
                     /translation="MIPARLLVVALMGLTAGFVTPALRASAKAGGEMTIDTSDGPRHA
                     LVIPAGNGARPTVIVLHGALGTGAGTARTTGFAEAAAKRGFTAVFPDGLGRQWNDGRA
                     GGPGGPDDIGFIRALVKRLVAEGDADPQRIYLAGISNGGMMSFALACKAGNLFAGIGT
                     VIANMPAGIEPCAARPMPVVMINGTADPIVPYAGGGVGLGGGRGEVWSVEQTANLFAR
                     LDRCGGQIEQPLPDRDPSDGTSVTRVAWDGCSAGTSVTLYRVEGGGHSLPGRPSLAPR
                     LLGLTNFDISGPGVILDAFTQSSGRML"
     gene            complement(5664..6911)
                     /locus_tag="VN968_15780"
     CDS             complement(5664..6911)
                     /locus_tag="VN968_15780"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXL30730.1"
                     /translation="MLDLGRFRRVLLVNDPDEIEHVLLGNAENYRKSIQQQRRLRPAL
                     GEGLLTAEDEAWRRSRRVAAPLFSPKAIAQLLDDMREAAEAMRDRWFARPGVAEPLDL
                     AAEFQRLTYEIVSRTVFSGALDADRARVHANMAIYFDTFGRIDLASILNLPSWLPTPA
                     ALRARPALAVFRSIVDRIVAQRRQDPGREVRDLLDRLMLAPDPKTNRKLPPATVADNV
                     LTFLAAGHETTGNALSWIFYLLSLYPETEEAVTAELRAAFGEGPPRRDLFGRLVMTRA
                     VIDEALRLYPPAPFIGREAICEDMLAGRPIKPGTQVLISPWIVHRHRLLWEEPDAFRP
                     ERFLPREKQSIRRGAFIPFGLGPRTCIGEGFAVQEMITVLAVILPAFRFRLADPRAVF
                     PEARITLHPRGGIPMIVTPREHG"
     gene            complement(7105..7425)
                     /locus_tag="VN968_15785"
     CDS             complement(7105..7425)
                     /locus_tag="VN968_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012382938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HXL30731.1"
                     /translation="MSHSVSLARFLAPAALAFCAATSPALAQSQPDPSQLSPRMRSEA
                     MALIRLCRGDYDRLCAGVQPGGGRILACLQSHASDLSASCAQAMPRAASLKTDAASAG
                     MLPK"
     gene            complement(7428..8354)
                     /locus_tag="VN968_15790"
     CDS             complement(7428..8354)
                     /locus_tag="VN968_15790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HXL30732.1"
                     /translation="MPGFSGLFASPAMRATILMVAIAFMGLEFGLSHLAHRADDTHDW
                     RESAVSFAVAIGQKAVRAAEAGLVAVPFAFAYQHRLFDFDQNSWIALAGLFFGSELIY
                     YWQHRASHRIRWMWATHAVHHSPTRLNLTAAIRLGWTGTISGNFLFFLPLALIGFHPL
                     AIVAMLGVNLLYQFFIHTELAPRLGPLESVLNTPAHHRVHHASNESCLDRNYGGILIV
                     FDRLFGTFAEAPENERLSYGLVGGVASLNPLRIAFGEWLAMADDLIRAASWSERWHAL
                     FGPPGASKAGRARDDTSLSPRKARHAFTTAGE"
BASE COUNT         1688 a         2567 c         2734 g         1587 t
ORIGIN      
        1 tcattagccc tgctaagcgc aacggcgctt ctcgctgtcg cttccgctac aaatgctcat
       61 gcgcaaggtt ggcgcaacga tggtggttcc ggctacggat atcgtccccg ctgtctgtcg
      121 agctatggca tcaacgaaag cctggcgaga cgtggcctat accccctcgc caatgtgggg
      181 agcgatccat atggaagata catctatatg cgggttgctc gggggaatga gcagtttgtc
      241 gtgaccgttg acggctgcac tggacaagtc atgcgataag ccagaggcgg ctatttcgca
      301 tcgccgagtg gtccatttgg ggactgatct ggggaccaca agctcctaac acagttgtat
      361 attaataaaa tcaggtactt atgtagtttt gctggcgaag cgaaagcgat tcgagctgcc
      421 ggtacgttct gctgccaagc gtgttcacgt ccgttgacgg ttcagcgaaa aaatccaggg
      481 agcgtggctt catatgcgag aggcgcggtc ccactcccgg gccgagatcg cgattgtcgc
      541 gatcatcccc gctaatctcg acgacatcaa cggcgccgtg caacgcggct gaaaagacct
      601 tcgctaacca accgcgtact cgagcgagag atagcgtagc ggcacgaaaa atatccgtta
      661 gggtttcaaa cgagacactg actggagacc gccgaaccgc aatacgctaa gtatttgaaa
      721 ttggtggcgc gccacccaga ataaaaactg cgaactctta tgtgattgag gtagctgtcg
      781 ctagttcgaa cgctcttccg aaggcatctt cagtgtcgct ttcgacctcc gtcttccaag
      841 aagaacatgg agaggcagcc acgcgggcag gcaaaacccg gcgacaatat gtatccagct
      901 catccagggt cgcacggctt cggaccccag ataatagagc ccgaaggccg tcatgatcag
      961 gagcgtattg agcgtgatca tcactgatcc cgaaaccagg ttattgccgc ttcgccaaga
     1021 gcgcagcatg tgcaccggga tcagggcgcc caacaacaac aatgtcacca tggcggcacc
     1081 gccatgtacc atcagcaggt tggcggcggc ttgctgccat atctcgtcgc tggagatatt
     1141 cttttgccaa tccgcgacaa gccaactggc cccgatcaag aacagaaccg tgacggccgc
     1201 atagatcgaa aaacgaaatg caggtttaag ccgcaaggtg cactgcgtga tgccagtcga
     1261 gggtgatatg aacgtcgccg tccgtcgaaa ttagtagcgc actcgcgttg tagtgctcaa
     1321 gcacctcagt tgcgtcagtg ccggatatca tgacaatttt cgtcaacgcg tcggcgacca
     1381 tgcaggtggg tgcgcgcacc gtagccgcat cgatggtgtc ggttggcttg cgggtgccgg
     1441 gatcgaccac tgccgaagcc gttgtctccg acgaccgaaa cggatcgaag cggcgcgccg
     1501 tcgacgcgat ggctccatcg gtgatctcga cgcgacagat cgggcggccc ggatcgcgcg
     1561 gatcgcgaat atggatcatt tgcgggtcgc ggccgaatac agcaagatcg ccgccagcgt
     1621 tcactatgcc ggattgcacg tcgaagcggc gcagggcctc gactgcccga tcgaccgcga
     1681 agcctttcgc aattcctccg agatcaatcc tgacatcggg gcttcggaag cggacggttt
     1741 ctcctggaaa caattcgatc gcatcaggca gttgcggccc ggatggaatt gggggatcgc
     1801 cggcggagcg aggcagaagt cccatcgtct gcaaggtcgg agcgacagcg atatcgaaaa
     1861 tgccgttcga gcggcgatgc atttcaaccg cggcttccag aacttcaaac gtccaggcgt
     1921 gaaccctggt tggccgaaga ccagcttggc ggttgaggcg gcttacgtcg ctgtccgccg
     1981 cgtgaaagct catcagccga tggacatttg cgacggcctc gaacgccgcg tcgacggctg
     2041 cttccatcgc gaatgcggcc tcacccgtca ccccgatctc cacgaaggtg ccaagcagcg
     2101 gtcgggcgcg gcgaatgcta tcggagccgg ttgccataga cagccagtat ccttttcacc
     2161 ccttcggtca gatgcgtcga tgacaacgtc gcgcctgcga tattgcgtat gtcgctgttg
     2221 atcctgagcg cgctgccgct ggcctttcct acgaactgcg aaagccagct cgggctgttc
     2281 acctccccgc cataggattc cctgtactgc aggatctcga ctttctgcac gacgccgctg
     2341 gtgctcagcg caaccgcata gtcgataaac aggtgcttgc cgacgacgcg gtcgaagaca
     2401 aaaaatccga tgccccgacc gccggcttcg gccttccaga cccggcccgc ctgcacttct
     2461 gtaaactgcg ttgccgacgg aaacgcctgc ttctgtgctt cgccgatcga gagatattgg
     2521 atggcatagg cgggcgacgc gatcgacgcg accgctaccg ccggtgctgc atatcgaacc
     2581 cacttcgtca tgcttccgtc atctcccgga tcaatctgga aaccgtcgtt ttgttaagcc
     2641 gccaagcctt ctccttggcc gtagctagaa gaaaaatccg actccgccaa ggaaagaatc
     2701 ggaaagcgcg cctacgatcg atttgtcgat caccctttga taggtggcct ttagcacgac
     2761 cttgggcgaa ggaaagacgg ctacgccggc ggtatggtac tgacgttgac ccgcgccggt
     2821 cggggtgttg tcgtcggtgc cggcgaagcc tgccgtttgg aagtcttgac gggtataccg
     2881 gtagaacggg acggcctccc attcgctgtt gatgatggtt cccagcggta cgtgatacgc
     2941 aacctccccc gagtaacccc acatcttctt gccaacgtta tttgtcgggt cgctatcatt
     3001 atttgcgcgc aggttttcgg gatggccaaa tcttgcctga acatactcgc cgcgcaattc
     3061 gagaccggta ccctgaaccc ggtagcggaa ttcagcatcg aacagcgcga ggctcgatcc
     3121 tccgagtatg ttaccgaggt cgtcgtgcgc tccacgcggg gtcgtgttcg gtgtgtagta
     3181 ggcgctcacg ctgccggcga atcccggcag gaagggcggc gcgaaatcca gttttccggc
     3241 ataggcgagg gtgttgctca gttggcggaa gtcgccgaca acgggccttg aattggcgag
     3301 cgcactcagt ccgtcgatgc ctgcggcgta gccggtcggg aagggcggaa cggcatttgc
     3361 gtccgtgcgc aatccgaagt cgtcgccgaa atcctcgacg ctcgaactga cctgaatttg
     3421 gtaactcagg ccgtcagcaa tccggccata gacgctgctt gacgggacat gccaggtcga
     3481 gggaatcaga ccgttataaa gttgcgggcg gttcacgctg taaaactgcg tgggctcgtg
     3541 atgctggttg atgtagccaa tcggcacgag atcgataccg ggcgaacgcc agttgaattg
     3601 gtccataacc ttgaagtcta cccagatttg ctcgatctcg gccgaaccgt gccgtttgtc
     3661 gtccgagtcg aatgcgatgc cgccgtgctc gaactcgatc tccgcattga agatgatgtt
     3721 gggagtgatc gcgtaggtcg ggagaagcac gatccggtga gcgtcgaagc ctttttgcca
     3781 ttgaccattg gcagccggat tttgctggga gccgaacttg atttcgccat acgctccgat
     3841 gctgagaccg ggaacgggtg agtcggcgag ccccacaatg ccattgtttg cgggcgccgt
     3901 cggggtcgga gatggcgcgg tgctcgcgat ttccgctctt gcggcgcgcg caggcgcggt
     3961 tggctgcgca gatgttgtgg gctgagcatt ggacggggct gcagccggcg gcgtcgaagg
     4021 tgaagcggct acaggcccgg cttgggcggc cgcctgtttc tgcttgagct ttccctcaag
     4081 catttgaatc cgctgctcca tcttttccag ttttttcagc agcgcgtcgt ttgcttcacc
     4141 gtccgctgcc caggcgccgc tgcagcctat gatggacatt gcagttgccg cgagcagcgc
     4201 ccttcgcctt gagcaacgaa ccggttggat cgagacaggc aaacgtgaaa actggtgcaa
     4261 cgacatgggt cccccaaaat ccgcggagat caaagtttcc gcgacatccg gggacctaac
     4321 accgttgcaa aacatttctg gaatcggaaa tggtttgctc gactggaatc tctctatttt
     4381 agaattagaa caaaaccaaa gtgatccgac attcgccgat cgtggccgtc tgaagcggac
     4441 agtgccctac gtcccgaacg cagtgaatat cttctaagtt gttgattttt cttgctacga
     4501 caggtgcttc cgccacgttt tgttcacggt cgtttcacct aattcgttgc gatttcgttg
     4561 cggcgttctc gggcagaacg gcgcctgctg ggggtcagat cttgccgctg cacatcccgt
     4621 gactcgtgac tgtcacaggt tcttttcggc gagttcgcga tggttacggg gaggcgcaac
     4681 cgcttgcgga gttcgacgcg atcgaggaag gcggcgaacc gcggccgcgc acggttggct
     4741 tgctataaca tccgaccact gctctgtgtg aacgcgtcga gaatgacgcc gggtcccgaa
     4801 atatcgaaat tggtcaagcc gaggagccgc ggcgcgagcg aaggacggcc cggaagcgaa
     4861 tgcccgccgc cttcgacgcg atagagcgtg accgacgttc cggccgagca gccgtcccag
     4921 gcgacgcgag tgaccgaggt gccgtccgag ggatcgcgat ccggcagcgg ctgttcgatt
     4981 tgcccgccgc atcggtcgag ccttgcgaac aggttagccg tctgttccac gctccagacc
     5041 tcgccgcggc cgccgccaag cccgacaccg ccgccggcgt aggggactat tggatcggcg
     5101 gtgccgttga tcatcaccac cggcatcggc cgcgcggcgc atggctcgat gcctgcgggc
     5161 atgttggcga tcaccgtgcc gatgccggcg aacagattgc cggccttgca tgcgagcgca
     5221 aagctcatca tgccgccatt ggagatgccg gcgagataga tccgctgcgg gtcggcatcg
     5281 ccttcggcca caagccgctt caccagcgcg cgaatgaagc cgatgtcgtc ggggccgccg
     5341 ggcccgccgg cgcggccgtc attccactgg cggccgagac catcgggaaa caccgcggtg
     5401 aatccacgtt tcgctgcggc ctcggcaaaa ccggtggtac gcgcggtgcc ggcgccggtg
     5461 ccaagcgcgc cgtgaagcac gatgacggtc ggccgggcgc cattgcctgc cggaatcacc
     5521 agcgcatggc gcggtccgtc cgaggtgtcg atcgtcatct cgccgccggc tttggccgac
     5581 gcgcgcaagg ccggcgtaac gaatccggca gtcagtccca tcaaggcgac aacaagcaat
     5641 cgtgcgggga tcatgggtgt ctcctaacca tgttcgcgag gggtaacgat catcgggatc
     5701 ccgccgcgcg ggtgcaacgt gatgcgcgct tcggggaaaa ccgcgcgcgg atcggccaag
     5761 cggaagcgga acgccggcag aatgacggca aggaccgtga tcatttcctg cacggcaaaa
     5821 ccttcgccga tgcaggtgcg tggcccgagc ccgaagggaa tgaaggcgcc gcgccggatg
     5881 ctctgctttt cgcgaggcag aaagcgttcg ggccggaagg catcgggctc ttcccagagg
     5941 agacgatgac gatgcacgat ccacggcgag atcaggacct gtgtgcctgg cttgatcgga
     6001 cgtccggcaa gcatgtcctc gcagatcgcc tcgcggccga tgaagggcgc cggcgggtag
     6061 aggcgcaagg cctcgtcgat caccgcgcgg gtcatgacca ggcggccgaa caggtcgcgc
     6121 cgcggtggac cttcgccgaa tgccgcgcgc agttcggcgg taacggcttc ttcagtctcc
     6181 gggtacagcg acagcagata gaagatccag gagagcgcat tgcccgtggt ctcatggccg
     6241 gcggcgagaa atgtcagcac attgtcggcg acggttgcgg gcggcaattt ccggttcgtc
     6301 ttcggatcag gcgccagcat caagcggtcg agcagatcgc gaacctcacg tccagggtcc
     6361 tgccgacgct gcgcgacgat ccggtcgacg atcgagcgga acacggccag cgccggccgt
     6421 gcccggagcg cagccggcgt cggcagccat gacggcagat tcaggatgct ggcgagatcg
     6481 atccggccga atgtgtcgaa gtagatcgcc atgttggcat ggacccgtgc gcggtcggcg
     6541 tcgagcgcgc ctgaaaatac cgtacgcgac acgatctcgt aggtcaggcg ctggaattca
     6601 gcggcaagat cgagcggctc ggccacgccg gggcgagcga accatcgatc gcgcatcgcc
     6661 tcagcggctt cgcgcatgtc gtccagcagc tgtgcgatcg ccttgggact gaacagaggc
     6721 gccgcgaccc ggcggctgcg ccgccacgct tcgtcctccg ccgtcagcag gccctcgccg
     6781 agcgcgggac gcaggcgccg ctgttgctgg atgctcttgc ggtagttctc ggcgttgcca
     6841 agcaggacat gctcgatctc gtcggggtcg ttgaccagga ggacacggcg aaaacgaccg
     6901 agatcgagca cgcttgctga taggcctccg tcgcaaaggc cgtgatcgga ttgctgcgca
     6961 acgcccggac ggaagcaatc aggcctggcc gggaccgccg cggctccggc gccgccggcc
     7021 tcggtccgac gccgcggacg gcgggcctgt ccggcaaggg gcgatctaga acagcgaaag
     7081 actgcgttgc catcgttacc tccgttactt gggcagcatg ccggcggagg cggcatcggt
     7141 cttcagcgag gccgcccgcg gcatggcctg ggcgcaggag gcgctgaggt ctgacgcatg
     7201 gctctgcagg caggccagga tgcgcccgcc tcccggctgc acgccggcgc acagccgatc
     7261 gtagtcgccg cggcagagcc gtatcagcgc catcgcctcg ctgcgcattc gcggcgacag
     7321 ctgcgaagga tcgggttgcg actgcgccaa tgcgggcgat gtggcggcac agaacgcaag
     7381 agcggcgggg gcgaggaaac gcgcgagcga gacagagtgg gacattttca ttctcctgct
     7441 gtggtaaatg cgtgccgagc cttgcgcggc gagagagatg tatcgtcgcg agcacgcccc
     7501 gccttcgatg caccgggtgg accgaacaac gcgtgccagc gctccgacca gcttgctgcc
     7561 cggatgagat cgtccgccat ggccagccat tcgccgaaag cgatccgcaa tgggttgaga
     7621 ctggcgacgc cgccgacgag gccatagctc agcctctcgt tctcgggcgc ttcggcaaaa
     7681 gtgccgaaca gccggtcgaa gacaatcagg atgccgccat agttgcgatc aaggcaggat
     7741 tcattggacg cgtggtggac gcgatgatgc gcgggcgtgt tcaggaccga ttcgagcggt
     7801 ccgagccgcg gcgcgagttc ggtgtgaatg aagaactgat agagaaggtt gacgcccagc
     7861 atcgcgacga tcgcgagcgg atggaagccg atcagggcga gcggcaaaaa gaacagaaaa
     7921 tttcccgaga tggtgccggt ccatcctagg cggatggccg cggtgaggtt cagcctcgtc
     7981 ggcgaatgat ggaccgcatg cgtcgcccac atccaacgga tacgatggga ggcgcgatgc
     8041 tgccagtagt agatcaattc gctgccgaaa aacaatccgg caagcgcgat ccaactgttc
     8101 tgatcgaagt cgaacagacg atgctgatag gcgaaggcga atggcacggc gacgagcccg
     8161 gcctccgccg cgcgcacggc tttctggccg atcgccacgg cgaacgacac cgcgctttct
     8221 cgccaatcgt gcgtgtcgtc cgctcgatgc gcgaggtggc tgaggccaaa ttcaagcccc
     8281 atgaaggcga tcgcgaccat caagatcgtc gctcgcatcg ctggactggc gaacaggccg
     8341 gaaaagccgg gcatggcgga acgccttcaa gacagagcgg taaggaaagc cggacgggct
     8401 gggcccccaa cgtcgggcgc ccagccggcc caacgacgat cagcgcgtga cggtgccctg
     8461 acgataaatg gtcaggccgt tcggccccgt cgtctgccgg ctgccggtgc aaacgccgcc
     8521 agcacacgac accgttcctt gccgggtcac gctgttgccg tacgggccgg tgcggg
//
LOCUS       DATNOI010000394         5110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_936102,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000394 DATNOI010000000
VERSION     DATNOI010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936102"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..722
                     /locus_tag="VN968_15795"
     CDS             24..722
                     /locus_tag="VN968_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30733.1"
                     /translation="MMAMSKKILEQEPSELEMLLPWHAAGTLNARDARRVDDALARDP
                     ELAKQYAVIREEYAETIHLNESLGAPSARAMQKLFAAIDGEPARNSSASLNLAARISE
                     FFAKLSPRTLALSASLGALALLLQAGVIGAILMKNQGASYQTASLSMNEPVTRNLGAG
                     RPPRALVRFAPDARVADITALLDTYQASIVDGAQGGLFQLQFGNKAMTKDEIAGLMSK
                     LQSEKTVSLAVPAQ"
     gene            881..2425
                     /locus_tag="VN968_15800"
     CDS             881..2425
                     /locus_tag="VN968_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXL30734.1"
                     /translation="MRSPNLNIQARIPTINPTVVPRIDPNIAGRMTPNLRTYPACSYA
                     YRNSDGECSSKPVLSTDGGGTAGTGGSGGASGKNKDKNKNSGPRRIVAQTVLNQRTIT
                     GEIVAEIDSSLSVTQTDELARRHGLARLQSQNFPLIGATIGLFRITDRRSAQTVSREL
                     ATEASIRSVQLNFRHFLQEQEAALTEGDPAQYALAKLRLPQAHTLARGANVTVAVIDS
                     GVDVKHPELANAIADSFDALGSKEGPHVHGTGIAGAIVSHARLMGSAPAARILAIRAF
                     AAAPNGGESTSYVILKALDYAAAHGAQIVNMSFAGPNDALIERGISAAVAKGIVMVAA
                     AGNAGAKSPPLYPAANRSVIAVSATDSQDRLFAASNRGGHIAVSAPGVDIFLPAPDEK
                     YQMTSGTSFSAAYVSGLAALLLERNPALKPDEVRAILMKTARDLGAPGRDDLFGAGEA
                     DAYAAVSAVAPAPATPVATVSDRPAGEVVPDRPDAPPTRALNQPPAAMASDKSVVGGA
                     VRPAAQ"
     gene            2899..3393
                     /locus_tag="VN968_15805"
     CDS             2899..3393
                     /locus_tag="VN968_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03809 family protein"
                     /protein_id="HXL30735.1"
                     /translation="MTHQIDVARGRDTVARWCALAEQRLEYLTELFETGRWRRFHNEL
                     AFLENIQEAKTAVEIWRDLSLREASRDHSSVDIAWLGRTGTAMPCRVTWLDPGHRMQP
                     RAAAADNPPASIVSIVPETDSHCSKEVRSNEVPSLPATKDASEPRSDMAAIAERYPLL
                     RIAL"
     gene            complement(3366..4085)
                     /locus_tag="VN968_15810"
     CDS             complement(3366..4085)
                     /locus_tag="VN968_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HXL30736.1"
                     /translation="MSQKLRILVTGFGPFPGAPFNPTQPLVARLVRLRRPALRDVELS
                     GHIFPVTYTAVDRELPELLASRQPHALLMFGLASRTPYVRVETRARNAVTTLWPDADH
                     ARLRKGSIAGGADALMFGPHVAKLLRAADGTGIDARTSHDAGSYLCNYLSWRAIEATS
                     GDTGPRLAAFVHVPALKRDGASQRKGAARRVTLEELVDAGEAMLMEMVRLTRQAVREA
                     ATPHHIAVTSAVPYSAMRSSG"
     gene            complement(4087..5076)
                     /gene="meaB"
                     /locus_tag="VN968_15815"
     CDS             complement(4087..5076)
                     /gene="meaB"
                     /locus_tag="VN968_15815"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HXL30737.1"
                     /translation="MTLPNTPPDIIVLTKDLRGGSRAALARAITLIESRRGDHQAAAR
                     DMVQALLPDTGKAIRVGITGSPGVGKSTTIDVLGMFLIERGHKVAVLAVDPSSARTGG
                     SILADKTRMARLAASDSAYIRPSPASGTLGGVAAKTREAMLLCEAAGFDVVLVETVGI
                     GQSETAVCDMTDFFLALMLPGAGDELQGIKKGLVELADLVAINKADGDNIERANLAAA
                     EYRGALHILSSRSEHWQPPVLTYSALTGTGIDALWQSILDHRSAMNASGEFAARRRLQ
                     QVKWMWSMLEQRMMARLRADPSVRAKVKKVEAEVADGRVTPALAAEQIAEMLR"
BASE COUNT          902 a         1673 c         1620 g          915 t
ORIGIN      
        1 gccggcgtag aacgaggctg gccatgatgg caatgagcaa gaaaatactg gagcaagagc
       61 ccagtgaatt agaaatgctg ttgccatggc atgcggctgg taccctgaat gcgcgcgatg
      121 cccgccgcgt cgacgacgcg ctcgcccgcg atccagagct tgccaagcaa tacgccgtga
      181 tccgggaaga atacgccgag accattcatc tcaacgaaag cctcggtgcg ccgtccgcgc
      241 gcgccatgca aaagctgttt gcggcaatcg atggcgagcc ggcacgcaat tcctcggcct
      301 cgctcaatct cgccgccagg atttcggagt ttttcgcaaa actctcgccg cggacactgg
      361 ccttgtcggc gagcctgggc gcgctggcgc tgttgttgca ggctggcgtt atcggcgcga
      421 tcctgatgaa gaaccagggc gcctcctacc agaccgcgtc attgagcatg aacgagcctg
      481 tcacccgcaa tttgggggcg ggccggccgc cgcgcgccct ggtacggttc gctccagacg
      541 ctcgcgtcgc cgacatcacg gcgctgctcg acacctatca ggcctcgatc gtcgatggcg
      601 cccagggcgg cctgttccag ctacagttcg gcaacaaggc gatgacgaag gatgagatcg
      661 ccggtctgat gagcaagctg caaagcgaga agaccgtcag tctcgcggtg cccgcgcagt
      721 aaggcgcttg gtgcctcctc catagcgcat aggccgaggt ctcatggtcc acgatcgtgc
      781 aaattggcgg gtgaagacgc ggcgcgcggc gttggccttg accgttgcgg ctttgccatt
      841 tgcgtatctc ggttgctcgg cgcttcaggc gcaaagcgtt atgcgttcgc ccaacctcaa
      901 catccaggcg cggattccga ccatcaaccc caccgtggtg ccgcgcatcg atcccaacat
      961 tgccggcaga atgacgccca atctccggac atatccggcg tgcagctacg cctaccgcaa
     1021 cagcgatggc gaatgctcca gtaagccggt gttgtcgacc gatggcggcg gtaccgcagg
     1081 taccgggggc agcggcgggg cgtcgggcaa gaacaaggac aagaacaaaa acagcggtcc
     1141 gcggcgcatt gttgcgcaga cagtactcaa ccagcgtacg atcacaggcg agatcgtcgc
     1201 cgagatcgac agctcgctgt ccgtcaccca gactgacgag ctggcgcggc gtcacggctt
     1261 ggcgcgtctg caatcgcaga attttccgct tataggcgcg accatcggct tgttccgcat
     1321 caccgatcgc cgatcggccc aaacagtgag ccgcgaactc gccaccgagg ccagcattcg
     1381 ctcggtgcag ctgaatttcc gtcatttcct gcaggagcag gaggcggcat tgaccgaggg
     1441 cgatcccgcg caatacgcgc ttgcgaagct tcgattgccc caggcgcata cgctggcccg
     1501 cggcgccaac gtcaccgttg cggtgatcga ttccggcgta gacgtcaaac atccggagct
     1561 cgccaacgcg attgcggact cattcgacgc gcttggcagc aaggaaggtc cgcacgtcca
     1621 cggcaccggc atcgccggcg cgatcgtgtc acacgcgcgg ctgatgggca gcgccccggc
     1681 agcacggatt ttggcaatcc gcgctttcgc ggcggccccg aatggcgggg agagcacgtc
     1741 gtatgtgatc ctgaaggcac tcgattacgc ggcggcgcac ggcgcacaga tcgtcaatat
     1801 gagcttcgcc ggtccgaacg atgcgttgat cgaacgcggg atctccgccg cagtcgcgaa
     1861 gggcatcgtg atggtcgccg cggctggcaa cgctggagcg aaatcgccgc cgctatatcc
     1921 tgccgccaat cggagcgtaa ttgcggtcag cgccaccgat tcccaggaca gattgtttgc
     1981 cgcatcgaac cgtggcggcc atatcgcggt gtcagctcct ggcgtcgata ttttcctgcc
     2041 cgcgccggac gaaaaatatc agatgacgtc cggcacctcg ttttccgccg cctatgtcag
     2101 cggcctcgcc gcgctgttgc tggagcgcaa tcctgccttg aagccggacg aggtccgcgc
     2161 gatcctgatg aaaactgccc gcgatctcgg cgcccccgga cgcgacgacc tgttcggcgc
     2221 cggggaagcc gatgcctatg ctgcggtttc ggcggtggcc cccgcgccgg cgacgcccgt
     2281 agccacggtt tcggacaggc ctgctggaga agttgttccg gaccggccgg acgctccccc
     2341 gacccgcgcg ctgaatcaac ccccggccgc catggcgtcc gacaagtcgg tcgttggcgg
     2401 agccgttcgg cccgccgcgc agtagttttc ctccaaaatc tcccgccaaa aaagttaaaa
     2461 aaatctgaag tccgtttgaa cgtgccgcga ccctcccgcg acacaataat tgtgggagcg
     2521 gttatccctc cccatcgagg ccgtattcgg cgcgagcgcc catccacccc aagcgcgccc
     2581 atatggcttg acccgtccgg ttgtcccccc ggacgggtct ttcgtttttg ggatattccg
     2641 ccgctccgct tgacgaaaat cggctttcgg gagccgcgcc cgcgtatttg cggccccctg
     2701 caactccgcc atgccggtag gtggcttgac tgtggattgg aaggaaaacc ccgccgttct
     2761 acggctttct gtcgatttcg gggctgcgcc agcgtcgccg gctggacaac gttaagtttt
     2821 ccacaaaata tatgtgtcac gtttgattga ttcgtgctcg ttgcgtccgc gtccgttgtt
     2881 ctccttgcgg gctggtccat gacacatcaa atcgacgtgg cacgcggtcg cgacaccgtg
     2941 gcgcgctggt gcgctcttgc cgaacagcgg ctggagtatc tcaccgaatt gttcgagacc
     3001 ggccgttggc gccgctttca caacgaactt gcatttctcg agaacatcca ggaggccaag
     3061 actgcggtag aaatctggcg cgatctgtcg ttgcgcgagg cctcgcggga tcattcgtcg
     3121 gtcgacatcg cctggcttgg ccgcaccggc acggccatgc cgtgccgcgt aacctggctg
     3181 gatccgggac atcggatgca gccccgggcg gcggctgcgg ataacccccc ggcatcgatt
     3241 gtttcgatcg tccccgaaac cgatagccat tgttcgaaag aagtgcgttc gaacgaagtg
     3301 ccttccctgc cggcgacgaa agacgcttcg gagccgagat cggatatggc ggcgatagct
     3361 gaacgctatc cgctgctgcg catcgcgctg taggggacgg cggacgtcac cgcgatgtgg
     3421 tgcggcgtcg ctgcttcccg caccgcctgc ctcgtcagcc tcaccatctc catgagcatg
     3481 gcttcgcctg catcgaccaa ctcttccagc gtgacccgtc gcgccgcccc tttgcgctgc
     3541 gaggcgccgt ctcgcttcag cgccggcacg tgcacgaagg cagcgaggcg ggggccggtg
     3601 tcgccggacg tcgcctcgat cgcacgccag ctcagataat tgcagagata gctgccggca
     3661 tcgtgcgagg tccgtgcgtc aatgccggtg ccatcggccg cgcgcagcag ttttgcgacg
     3721 tgagggccga acatcaaggc gtcggcgccc ccggcgatcg agcccttgcg caagcgcgca
     3781 tggtcggcgt cgggccacag cgtggtgacg gcattgcggg cgcgagtctc gacccgcaca
     3841 tagggcgtgc gggaagcaag accgaacatc agcagcgcat gaggctgcct cgacgccagc
     3901 agttcgggca gttcacgatc gacggcggtg taggtcaccg gaaagatgtg gcccgacagt
     3961 tcgacgtcgc ggagcgcggg gcggcgcagc cgcaccagcc gagccaccag cggctgcgtc
     4021 gggttaaagg gcgctccggg aaacgggccg aagccggtga cgaggatacg gagtttctgg
     4081 ctcatctcat ctcagcatct cggcgatctg ctctgccgcc agcgccggcg taacgcggcc
     4141 gtcggccact tcggcttcga ccttcttcac tttggcacgc acggaaggat cggcgcgcag
     4201 ccgcgccatc atccgctgct ccagcatcga ccacatccac ttcacctgct gcagcctgcg
     4261 ccgtgccgcg aactcgcctg aggcattcat tgcggagcgg tgatcgagaa tactttgcca
     4321 gagcgcgtcg atgccggtgc cggtgagcgc cgaataggtc agcaccggtg gctgccagtg
     4381 ctccgagcgc gagctgagga tgtgcagcgc gcctcgatat tcggccgccg caagattggc
     4441 ccgttcgatg ttgtcgccat cggccttgtt gatcgcgacc aggtcggcga gttcgaccag
     4501 gcccttcttg atgccctgca actcgtcgcc ggcacccggc agcatcagcg ccaaaaagaa
     4561 atcggtcatg tcgcacaccg cggtctcgga ttgcccgatg ccgacggttt ccacgagcac
     4621 cacgtcaaaa cccgcggcct cgcacagcag cattgcctcg cgggttttcg cggcgacgcc
     4681 gccgagcgtg cccgaggccg gcgagggccg gatgtaggcg ctgtccgaag cggcgagccg
     4741 tgccatccgc gtcttgtcgg cgagaatcga gccgcccgtg cgcgcagagg aggggtccac
     4801 cgcgagcacc gctaccttgt ggccgcgctc gatcaggaac atcccgagca catcgatggt
     4861 ggtggatttg ccgacgccgg gcgagccggt gataccgacg cggatcgcct tgccggtgtc
     4921 cggcagcagc gcctgcacca tatcgcgcgc cgccgcctga tgatcgccac gccggctttc
     4981 gatcaacgtg atcgcccgcg ccagcgccgc gcggctgccg ccgcggaggt cctttgtcag
     5041 gacgatgatg tcaggggggg tgttcggtag ggtcatgcct tgctttacaa cgccgacacg
     5101 tggcaggcga
//
LOCUS       DATNOI010000395         3392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1075422,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000395 DATNOI010000000
VERSION     DATNOI010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075422"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..409)
                     /locus_tag="VN968_15820"
     CDS             complement(107..409)
                     /locus_tag="VN968_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30738.1"
                     /translation="MTRFIALSALATALIIGAAADANAWTRTGSVTGPRGTSSVQGSG
                     SCAGGTCSRSITRTGPYGNSVTRQGTVSCAGGVCTGSRQTTGPNGQTIYRQGTVTR"
     gene            599..1324
                     /locus_tag="VN968_15825"
     CDS             599..1324
                     /locus_tag="VN968_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL30739.1"
                     /translation="MTMQGATILMVEDDPEISRLVAEVLRKEGFEIEISETGEAMDAV
                     LRRLRPDLIVLDLMLPGEDGLSICRRLRQADNVPILMLTAKSDEIDRVVGLEMGADDY
                     LTKPFGPRELLARIRALLRRAQGAPAPPASRRFAFDRFVLDLDARAVERTGDDGGSLQ
                     LTTAEFDLLACFVQRPRRVLTRDQILDWTRGRSVEPFDRTIDMLISRLRKKLGAASPG
                     SNLISTVRNGGYLFTATVARVSS"
     gene            1321..2682
                     /locus_tag="VN968_15830"
     CDS             1321..2682
                     /locus_tag="VN968_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL30740.1"
                     /translation="MTRLTLIMRVTLILLVGLSALWIVVIAMFYRSSLATNEGARPAP
                     ARLAALAELIEHASPAERSLLLQAVASDRLSARVEAEQQPASPLTPPIDAALQNTYAK
                     ALDGRSLSLVASSKPPHGRWFPRLSKAPWNALEFRIGLRTGEILVVDTRTRLVVTPFG
                     LPVGFGAGLFGTIVALLALLVMQRETRPLARLAAAADQMDLSGSPVALPDVRRSAPEI
                     RAVVTAFGRMQTRLAELLSARMALVGGIAHDVRTFATRLRLRIDRIPEDAERQRAIAD
                     IEDMIRLLDDALLASRTGAGELAQELIDMDELVRAEAEDRRAHGAVVGYAAPAKPCGE
                     LAVLGDRLALRRVVANLLDNAIKYGGSAQITLARQEDWAVLTIDDEGPGIPPEKRLAM
                     FEPFTRLDSSRNRKTGGAGLGLAVARSLTEAHGGRIEIADAVRGTRIVVRLPLFARGS
                     PLG"
     gene            complement(2840..>3392)
                     /locus_tag="VN968_15835"
     CDS             complement(2840..>3392)
                     /locus_tag="VN968_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkyl sulfatase C-terminal domain-containing
                     protein"
                     /protein_id="HXL30741.1"
                     /translation="QVVFADPANRAARELGADALEQLGFQAEAATWRNAYLVGAMELR
                     NGVPKILGNNTANADTLKALNNEMFFDFLGVRLNAAKAEGKTMVINWSFTDSNQRIAL
                     NLDNSALTYVAGKQAPNADATVTLARGTLDAVTLQRTTFADAVKAGLVKIDGDPRKLD
                     ELMSMLDTFKVMFEVVEPKPSGQ"
BASE COUNT          550 a         1098 c         1095 g          649 t
ORIGIN      
        1 aggccggaaa agccgggcat gacgggttgc cttcaagaca gagcgggaag gaaaagcggg
       61 actggctgag cgcccaacgt cgggcaccca gccggcccaa cgacgatcag cgcgtgacgg
      121 tgccctgacg ataaatggtc tggccgttcg gccccgtcgt ctgccggctg ccggtgcaaa
      181 cgccgccagc acacgacacc gttccttgcc gggtcacgct gttgccgtac gggccggtgc
      241 gggtgatgct tcggctgcag gtgccgccgg cacagcttcc ggacccctgc acgctcgacg
      301 ttccgcgcgg accggtcacc gatccggtgc gggtccaggc gttggcgtca gcggcggcgc
      361 cgatgatcaa ggcggttgcg agggcagaaa gggcgatgaa cctggtcata tcgatctcct
      421 ttgggcttgc gacggcccta ctgccgccga tcacgcggac gaaaatgacg cgaccaggtc
      481 aaccgcgtat cgacggcaca tatcgttgtt tgtcgtttgg tcaacgccgt gtaacaggat
      541 gtaacaatgc ggcttctccc cttgtcaccg ggtgcattgt cgatggacag tcgacggaat
      601 gacgatgcag ggcgccacaa tattgatggt ggaggacgat ccggagatca gtcggctcgt
      661 ggcggaagtc ctgcgcaagg aaggtttcga gatcgagatc tccgaaaccg gcgaggccat
      721 ggatgccgtc ttgcggcgtt tgcggcccga tctcatcgtc ctcgacctca tgctgcccgg
      781 tgaagacggc ctgtcgatct gccgaaggtt gcggcaggcc gacaacgttc cgattctgat
      841 gctgactgcc aagagcgacg agatcgatcg cgtggtcggc ctcgaaatgg gcgccgacga
      901 ctatctgaca aaaccctttg gcccgcgcga actgctcgcc cggattcgcg cgctgctgcg
      961 ccgtgcgcaa ggcgcgccag ctcccccggc aagccgccgt ttcgccttcg atcgctttgt
     1021 gctcgatctc gatgcgcgcg ccgtcgagcg aaccggcgat gacgggggat cgctgcaact
     1081 gaccacggcc gagttcgatc tgctggcctg cttcgtgcag cgaccgcggc gagtcctgac
     1141 gcgcgaccag atcctggact ggacccgcgg ccgatcggtc gagcccttcg accgcaccat
     1201 cgacatgctg atttcccggc tgcgcaagaa gctcggcgcg gccagtcccg gctccaacct
     1261 gatcagcacc gtgcgcaacg gcggatatct gttcacggcc accgtagcgc gcgtctcgtc
     1321 atgacgcggc tcacgctcat catgcgggtc acgctgattc tcctggtcgg gctgtcggcg
     1381 ctctggatcg tggtgatcgc gatgttctat cgttcgtcgc ttgcgacgaa cgagggcgcg
     1441 cgtccggcgc cggctcggct ggccgcgctt gccgagctga tcgaacatgc ttcacccgcc
     1501 gagcgctccc tcctgcttca ggccgtcgcg tcggatcgcc tttcagcgcg cgtggaagcg
     1561 gagcaacaac ctgcctcgcc gcttaccccc ccgatcgacg ccgcgttgca gaacacctat
     1621 gccaaggccc tggatggaag atcgctgagc ctggtcgcgt cgtcgaaacc tccccatggg
     1681 cggtggtttc cgcggctgtc gaaggcgccc tggaatgccc tggaattcag gattggtctg
     1741 cgaaccggcg agattcttgt cgtcgataca cgaacgcggc tggtggtgac gccgttcggc
     1801 cttcccgtcg gcttcggagc cggcctgttc ggcacgatcg tggcgctgct cgcactcctc
     1861 gtcatgcaac gcgagacccg tcctctcgcc cggcttgctg ccgccgccga tcagatggat
     1921 ctttccggaa gccccgtggc gttgccggat gtgaggcgca gcgcgccgga aattcgcgcc
     1981 gtcgtcaccg ccttcggccg gatgcagacg cgtctggcgg aattgctgtc ggcccgcatg
     2041 gcgctcgtgg gcggcatcgc ccatgacgtc cgcacctttg cgacgcggtt gcggcttcgc
     2101 atcgatcgta tccctgagga tgcggagcgc cagcgcgcca tcgccgatat cgaggacatg
     2161 atccgcctgc tcgacgatgc cctgctggcg agccgcaccg gcgcgggcga gcttgcgcag
     2221 gaactgatcg acatggacga attggtccgc gcggaagctg aagatcggcg cgcccatggc
     2281 gccgttgtcg gctatgcggc gcctgcgaag ccatgcggcg aactcgccgt gctcggcgac
     2341 cgcctggcct tgcgccgtgt cgttgccaac cttcttgaca acgcgatcaa atatggcggg
     2401 tccgcccaaa tcacgcttgc ccggcaagag gattgggcgg tgctgacgat cgacgacgag
     2461 ggacccggga ttccgccgga aaagcggctc gcgatgttcg agcccttcac gcgcctcgat
     2521 agctcgcgaa accgcaagac cggcggcgcc ggtctcggcc tcgccgtcgc gcgatcgctg
     2581 acggaggcgc atggcggccg gatcgaaatc gccgacgccg tacgcggcac gcggatcgtc
     2641 gtccgccttc cgctgttcgc gcgtggctca cctctcggct agagcgggat gacttttctt
     2701 cgaatcgtca ttccgctcca tctttttgtt tgagcatgat ctccgcgcaa acgcttcgcg
     2761 tttgtcgcga gggaaaaccg gtacccactt ttccggatca tgctctaagc gcggcaggtt
     2821 gaggcgcgtc cggcacgcct cattgaccgc tcggctttgg ttcgaccacc tcgaacatca
     2881 ccttgaaggt gtcgagcatc gacatgagtt catcgagctt gcgcgggtcg ccgtcgatct
     2941 tgaccaggcc cgctttcacc gcatcggcga atgtggtccg ctgcagggtg acagcatcaa
     3001 gcgtaccgcg cgccagcgta acggtcgcgt cggcattggg cgcttgcttt cccgccacat
     3061 aggtcagtgc ggagttgtcg aggttcagtg cgatcctttg atttgaatcg gtgaaactcc
     3121 agttgatcac catggtcttg ccttcggcct tggcggcatt caaccgcacg ccgagaaagt
     3181 cgaagaacat ctcgttgttg agggccttca gcgtatcggc attggcggtg ttgtttccga
     3241 gaatcttggg cacgccgttg cgcagctcca tcgcgccgac gagataggcg ttgcgccaag
     3301 tggcggcctc ggcctgaaag ccgagttgct ccagcgcgtc ggcgcccaat tcgcgcgcgg
     3361 cgcggttagc gggatcggcg aacaccacct gg
//
LOCUS       DATNOI010000396         6445 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2424312,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000396 DATNOI010000000
VERSION     DATNOI010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2424312"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VN968_15840"
     CDS             <1..207
                     /locus_tag="VN968_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HXL30742.1"
                     /translation="EANPGDFIFVPPYVPHQEINASRDEVLECVLVRSDGEAVAINLD
                     IEPVEKPETVLWVDPVHRDPAETK"
     gene            416..847
                     /locus_tag="VN968_15845"
     CDS             416..847
                     /locus_tag="VN968_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL30743.1"
                     /translation="MAKLIFVNLPVADLARATAFYQAVGAEKNEQFSDATASCMVFSE
                     TIHAMLLTHDKFRQFTPKKIADAKSSSEVLICISADSRDAVDDMVRKAETAGGGVDPC
                     PKQDYGFMYGRSFEDPDGHIWEVMWMDVAAASAAQPATADA"
     gene            878..1363
                     /locus_tag="VN968_15850"
     CDS             878..1363
                     /locus_tag="VN968_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL30744.1"
                     /translation="MSKVSPCLWFDGEAEEAAKFYVSLLPDSRIGNIQKNAVDSPAGK
                     AGSVLVVDFTLAGQRFMALNGGKPFDFTHAVSFKIDCADQAEVDRLWDAMSDGGSVER
                     CGWLKDRYGVSWQIVPTALVQYLGGPDRAGAQRAMQAMLGMVKLDIAGLKRAYEGKSA
                     A"
     gene            complement(1548..3242)
                     /locus_tag="VN968_15855"
     CDS             complement(1548..3242)
                     /locus_tag="VN968_15855"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXL30745.1"
                     /translation="MSQTVAGQLVAVLEQIGVKQIFGLIGDSLNPLADAVRQSQIEWI
                     GVRHEEGAALAAGGQAKLTGQLAVCAGTTGPGSNHLVAGLYEASRDHAPVLALSGEMP
                     RPLQGTDYIQTTEPDLLFRDVSLYTETIATAAQAPGVIHQAIAAAYAGPGVAHLTLPQ
                     DVLSTKAEGAVSSLATLRPHSEFPAAEGDVARIVQQIDRAGSVVIMCGAGCRGAADSL
                     RALSDRLKAPLVHTLRGKELMAYDDPRWMGGLGMIGTKAVYHAVQNCDLLVMIGTDYP
                     YSNFLPTRGSVIQIDERPQVLGRRAPTALGVTGSARPTLKLLLERVSAKTDTRFWDKV
                     TNERRKWDEMLDKQSDLKRSKDRIHPQAVARAVSDAARRDAIFVLDTGLNTLWSGNWI
                     RQTGSQRIIGSFNNAAVGTALGQANGVQVLDRSRQVIALTGDGGFNMLMGEFMTAVHH
                     RLPVKAIVYNNSALGLITLEAESIGLVPFREAIEFPNPDFAALARACGGHGFTARQPG
                     ELKTALSEALAVDGPAIVDAMVVADEMPNMPHIDLEQVGHYAVAKIKEAVLAVTGG"
     gene            complement(3498..4139)
                     /locus_tag="VN968_15860"
     CDS             complement(3498..4139)
                     /locus_tag="VN968_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXL30746.1"
                     /translation="MTRLRKFSSLKAIALSAALSMTAGLAIADSKNVSSDQILSALQP
                     KQLTRGMSAGPQADPAVKAKEISFIKTLRNRKTRSLSLGEREEILAITSDKPKIDLDI
                     QFDYNSDKITTTSMPSVQALGKALSDANLKGSTFVVAGHTDAIGGEAYNQDLSERRAD
                     TIKRYLTEKYGINGTDLVTVGYGKTKPKDLNAPMDPVNRRVQVVNMDTKTASK"
     gene            4555..4785
                     /locus_tag="VN968_15865"
     CDS             4555..4785
                     /locus_tag="VN968_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30747.1"
                     /translation="MPLLIGAAMIAAAILLSTLITAVGTRFTGMESPNEENLWLIDRL
                     TGSVYKCQAPERGKAACEAEVATGSIGERSKR"
     gene            4963..5229
                     /locus_tag="VN968_15870"
     CDS             4963..5229
                     /locus_tag="VN968_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30748.1"
                     /translation="MRILIIAAASLALALTPAAAKTKAKATATACSGESMAKTSAAMA
                     AMPYSQGRMAMDKEMGMVNTEISKGNMRAACAHYIKVQKMSSAK"
     gene            5340..>6445
                     /locus_tag="VN968_15875"
     CDS             5340..>6445
                     /locus_tag="VN968_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HXL30749.1"
                     /translation="MSPARKSHPLSRAEARRIWLRAQRLETAAPFGEGAQATAAAVGH
                     LGYVQIDTINVIERSHHHILWTRIPEYRRADLRQAQGVDRSVFEYWTHALSYVPTRDM
                     RFFVPAMRRHQREGHKWLSSVKPADLRKVLRLIRRDGALTIRDIDDDVLVDKEHLWAS
                     RKPSKRALQLLFYTGVLTISERNGMLKTYELMARHFGWDKPPKGASAGEATAYLLDRA
                     LRAQGVVSLDSICHLDAPSKPSVRRLIEARMRRKELVQVALEGAGKQEHWARPETLEN
                     SGGGPSELVHILSPFDPLIIQRKRTHLFFGYEHRFEAYVPKEKRVFGYFALPVLVGDD
                     IVAALDLKTDRKSRKLLLQKWNWVGNGVQRGARK"
BASE COUNT         1205 a         1985 c         2069 g         1186 t
ORIGIN      
        1 gaggccaatc ccggcgactt catcttcgtc ccgccctacg tgccgcatca ggagatcaac
       61 gccagccgcg atgaggtgct ggaatgcgtg ctggtgcgca gcgacggcga ggcggtcgcg
      121 atcaacctcg acatcgagcc ggtggagaag cccgaaaccg tgctgtgggt cgatcccgtg
      181 catcgcgatc cggcggagac gaagtgacga cgtcattccg gggcgacgca tgagcgtcga
      241 acccggaatc cagggattcc ccgatgtgca attgcacctc tgaggtccag tccttcggac
      301 catcccgaaa tgacgtcgga ccaaccgatc acgtccataa aaaaaatcaa aatcggctgt
      361 cggatcggca cccgcccgtt cgtccttcgg ccgaaaccgc caaaaaggag ctgccatggc
      421 caaactgatc ttcgtcaatc tgccggtcgc cgacctcgcc cgcgccaccg ccttctatca
      481 ggccgtcggc gccgagaaga acgagcaatt ctctgacgca accgcatcct gcatggtgtt
      541 ttccgagact atccacgcca tgctgctgac ccacgacaag ttccgccagt tcacgccgaa
      601 gaagatcgcc gatgcgaaga gttcaagcga ggtgctgatc tgtatctccg ccgacagccg
      661 ggacgccgtg gacgacatgg tccgcaaggc agagaccgcc ggcggcggcg tcgatccgtg
      721 cccgaagcag gactatggct ttatgtacgg ccgcagtttc gaggatcccg acggccacat
      781 ctgggaagtg atgtggatgg atgtcgctgc ggcgagcgca gcgcagcccg caacggccga
      841 cgcctgatct caaacccatc acgcaaggag ctagctcatg tcaaaagtct caccctgcct
      901 gtggttcgac ggcgaggccg aggaggccgc aaagttctat gtttcattgc tgcccgactc
      961 caggatcgga aacatccaga agaacgccgt cgatagcccc gccggcaagg caggttcggt
     1021 gctggtggtg gatttcacgc tggcaggcca gcgcttcatg gcgttgaacg gcggcaaacc
     1081 tttcgacttc acccacgcgg tctcgttcaa gatcgactgc gccgatcagg ccgaggtcga
     1141 ccggttgtgg gatgccatgt ctgacggcgg gtcggtggaa cgatgcggct ggctcaagga
     1201 ccgctacggc gtatcctggc aaatcgtgcc gaccgcgctc gtgcaatatc tcggcggccc
     1261 cgaccgcgcg ggcgcgcagc gggcgatgca ggcgatgttg ggtatggtga aactcgacat
     1321 cgcaggcctc aaacgggcct acgaaggcaa gtcggccgcg tgaaggcgtg ggttgccgct
     1381 gacccatttt gcaacacggt ggcctcatcc ttcgagacgc cgcgcgaact tgagcggctc
     1441 ctcggaatgg ggttcttccc agaccctcgt ggtgaggagt gcggcaacgc cgcgcgtctc
     1501 gagccacaag gccgcgatgt gcgatcgtgg ttcgacccgc cggtaatcta gccgccggtg
     1561 acggcgagaa cggcttcctt gatcttggcc acggcgtagt ggccgacctg ctcgagatcg
     1621 atatgcggca tgttcggcat ctcgtcggcg acgaccatgg cgtcgacgat ggccgggccg
     1681 tcgacggcaa gcgcttcgct cagcgccgtc ttcaattcac ccggctgtcg cgccgtaaat
     1741 ccgtgaccgc cgcaggcgcg ggccaacgcc gcgaagtcgg gattcggaaa ctcgatggct
     1801 tcgcggaacg gcacgagccc aatgctctcg gcttccagcg tgatcagtcc gagcgccgaa
     1861 ttgttgtaga cgatggcctt gaccggcaac cggtgatgta ccgcggtcat gaattcgccc
     1921 atcagcatgt tgaacccgcc gtcgccggtc agcgcaatca cttggcgcga gcgatccagc
     1981 acttgcacgc cattggcctg gccaagcgcg gtgccaacgg cggcgttgtt gaacgagccg
     2041 atgatccgct gcgatccggt ttgccgaatc cagttccccg accacagcgt gttcaagccg
     2101 gtgtcgagaa caaagatcgc atcgcggcgt gccgcgtcac tgaccgcgcg ggctacggct
     2161 tgcgggtgaa tgcggtcctt gctccgtttc agatcggatt gcttgtccag catctcatcc
     2221 catttccgcc gctcgttagt gaccttgtcc cagaaccggg tgtcggtctt ggccgagacg
     2281 cgttcgagca atagcttcag ggttggacgc gccgatccgg tgacgccgag cgccgtcggc
     2341 gcccttcgcc ccagcacctg cggccgttcg tcgatctgaa tcacgctgcc cctggtcggc
     2401 aggaaattgg agtaggggta gtcggtgccg atcatcacca gcaggtcgca attctgcacg
     2461 gcgtgataga cggccttggt gccgatcatg cccagcccgc ccatccagcg cggatcgtcg
     2521 taagccatca gctccttgcc tcgcagcgtg tggaccagcg gcgctttcag ccggtctgac
     2581 agggcgcgca gcgaatcggc agcgccgcga catccggcgc cacacatgat caccacgctg
     2641 ccggcgcggt cgatctgttg gacgatcctg gcgacgtcgc cttccgcggc ggggaactcg
     2701 gagtgcggtc tcagggtggc cagactcgac acggcgcctt cagccttcgt cgacagcacg
     2761 tcctgaggaa gcgtgaggtg cgcgacgccg gggccggcat aggcggccgc gatggcctgg
     2821 tgaatgacgc cgggcgcttg tgccgcggtc gcgatggttt cggtgtagag cgagacgtcg
     2881 cgaaacaaca ggtccggttc ggtggtctgg atgtagtcgg tgccctgcaa tgggcgcggc
     2941 atctcgccgg acagcgccag caccggggca tggtcacggc tcgcctcata gagtcccgcg
     3001 accagatgat tgctgccggg gccggtggtg ccggcgcaaa ccgcaagctg cccggtgagc
     3061 ttggcctggc cgccggcggc cagcgccgcg ccctcttcgt gccggacccc gatccattcg
     3121 atctggctct gcctcaccgc atcggcgagc ggattgaggg aatcgccgat caatccgaag
     3181 atctgtttga cgccgatctg ttcgagcacg gcgaccagtt ggcccgcaac cgtctgtgac
     3241 atggtccgct cctgaagtgg tacgcccaat cgcggcgagc aggcccgccg cattcgatat
     3301 cgccataatc tggaaatcga atgcagcggt acggtgacgt ccgggtggca acatgttgtg
     3361 atgccggctg ctcgcgattg gcagggaacg gagtcgcggt tgccatatcc gaaaatgaac
     3421 gcaagactga cgtgccggga gagcgtgcgc aaatccgccc gccgcggggc ggcgagcgga
     3481 tttgcatttt gcaagtgtca cttcgacgcg gtcttggtgt ccatgttgac gacctggacg
     3541 cggcggttga ccgggtccat cggcgcgttc agatccttcg gcttggtctt gccatagccg
     3601 acggtgacca gatcggtgcc attgatgccg tatttctcgg tgaggtaccg tttgatggtg
     3661 tcggcgcggc gctccgagag gtcctggttg taggcttcgc cgccgatcgc atcggtgtgg
     3721 ccggcgacca caaatgtcga acccttgagg ttggcatccg agagcgcctt gccgagcgcc
     3781 tgtacggaag gcatcgaggt cgtggtgatc ttgtccgaat tgtagtcgaa ctgaatatcg
     3841 agatcgattt tcggcttgtc cgaggtgatc gccaggattt cttcgcgctc gccgagcgac
     3901 agcgaccggg tcttcctgtt gcgcagcgtc ttgatgaagc tgatttcctt ggccttgaca
     3961 gcgggatcgg cctgcggccc cgccgacatg ccacgcgtca gctgcttggg ctgcagggca
     4021 ctcaggattt gatcgcttga gacattcttg ctgtcggcaa tggcaagacc ggccgtcatc
     4081 gacagcgcgg cactcagcgc aatcgccttt aggctggaaa atttgcgaag cctggtcatc
     4141 gtcttgatcc tcgctgttga cgcctgatag cacttggatg ctgcgaggat agaggaggtt
     4201 caatgcggcg atgtgatctg ggtcacgttg ggcgaaaggg gcggcggctt gcgtcgttac
     4261 aaagcggggc gggagccgtc atgatcactg gggtcgggcg aaccccgccg gactgttttg
     4321 ctggctcaaa gcgtggaact ggtccagcag cctcgcggca acgttccgcg gtcgcaaagc
     4381 cgtcgccgga gcgccgatgt gctgacaata attcggccag atgcgcgttt aacaataccg
     4441 ctcgcaaaaa aagcaaagac agcgaaagcg accggggcct tgtttcagtc ttcgaaaaga
     4501 caaagcgaat atcaggggta tcgtggggcc acgccggaac gcatccaaaa cccgatgccc
     4561 ctcctgatcg gcgcagccat gatcgccgcg gcgatcttgc tgtcgacgct aattacggct
     4621 gtcggaacca gattcaccgg catggagagt cccaacgagg agaatctgtg gctgatcgac
     4681 cggctgaccg gcagcgttta caaatgccag gctcccgagc gcggcaaagc cgcctgcgag
     4741 gccgaggtcg caacgggtag cattggcgag cggtccaagc gctgaccagg ttggagcgga
     4801 gcgcgtgtcg gccgcgaacg ccccggtatc ccggcgcggc ggaatttagt ctttcgcgct
     4861 gcatcgcagc acacaagaaa tgtcgtctgt tggaaacgga tcggtgccct gcgcgtattc
     4921 cgacagccag cactgagctg gtttcattct tagggaggtc cgatgaggat tttgatcatt
     4981 gcagccgcct ctttagctct cgcccttacg cccgccgccg cgaagacaaa agcgaaggca
     5041 acggcaacgg catgctcggg agaaagcatg gccaagacca gcgccgcgat ggcggccatg
     5101 ccgtacagcc agggccggat ggccatggac aaggaaatgg gaatggtcaa caccgaaata
     5161 agcaagggca acatgcgcgc agcttgcgcg cactacatca aggtccagaa gatgagctcg
     5221 gcgaaataag accgggaatg taatggctca gccgcgctgc ccctgggcgg cgcggctttt
     5281 tcctgccaac tgaaatctgc gtctgctccg ccccgcgaat ccggctacat tcgcgcacca
     5341 tgtcccccgc acgtaaatcg catcccctgt ccagggccga ggccaggcgg atctggctcc
     5401 gcgcgcagcg gctcgagact gcggcgccgt tcggcgaggg cgcgcaggcg accgccgccg
     5461 ccgtcggaca tctgggttac gtgcagatcg acaccattaa cgtcatcgag cgcagccacc
     5521 accatattct ctggacccgg atccccgaat acaggcgcgc cgatctgcgc caggcccagg
     5581 gcgtcgacag aagcgtgttc gaatactgga ctcatgcgct gtcctatgtg ccgaccaggg
     5641 atatgcgctt ctttgttccc gcgatgcgtc gtcaccagcg cgagggccat aaatggctct
     5701 cttcggtcaa acctgcggat ctgcgcaagg tgctgaggct gatccggcgc gacggcgccc
     5761 tgacgatccg cgacatcgac gatgacgtgc tggtcgacaa ggaacacctt tgggcgagcc
     5821 gcaagccctc gaagcgggcg ctgcagcttc tgttctatac cggcgtgctc acgatctcgg
     5881 agcgcaatgg catgctcaag acctacgagc tgatggcgcg gcatttcggc tgggacaagc
     5941 cgccgaaggg cgcttcggcc ggggaagcca ccgcctatct gcttgaccgc gcccttcgcg
     6001 cgcaaggcgt cgtcagcctg gattcgatct gtcatctcga tgcgccgagc aagccttcgg
     6061 tccggcgctt gatcgaggca agaatgcgcc gcaaggagtt ggtacaggtc gcgctggaag
     6121 gcgccggcaa gcaggagcat tgggcgcggc ccgagacgct ggagaactcc ggcggcgggc
     6181 catcggagct ggtgcacatt ctctcgccgt tcgatccgct gatcatccag cgcaagcgca
     6241 cccatctttt cttcggctac gagcatcgct tcgaggccta cgtgccgaag gagaaacggg
     6301 tgttcggcta tttcgcgctg ccggtgctgg tcggcgacga catcgtcgcc gcgctcgatc
     6361 tcaagaccga tcgcaaaagc cgaaaactgc tgctgcagaa atggaattgg gtcggcaacg
     6421 gtgtgcagcg cggcgcgcgc aagga
//
LOCUS       DATNOI010000397        20786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1996658,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000397 DATNOI010000000
VERSION     DATNOI010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 20786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20786
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996658"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VN968_15880"
     CDS             complement(<1..1295)
                     /locus_tag="VN968_15880"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30750.1"
                     /translation="MRRSVARCLSGLVLALSVHLSTSGVSFAQQPKKGGTLVYASVSG
                     PGTLDPYVASSSVELEVINNVFEGLVTLDANNATRTMLASKAMASEDFKTYSFELRKG
                     VKFHNGQEMTSADVKASFERYRRVSPNAKNLEGVDHFDTPDPYSFVIVLRQPNVVLLD
                     VLKSPIYPLMILPESQKDKPARGVDLIGTGPYMMGEWVKDSHLVIKRFDDYVADASAP
                     GRDGYGGRKTVYLDAVRYRFMPEATTRIAALQNGEAQLISTIPNELSARITERKDLAV
                     RELFPLLGSYIIVNSQKGLTANVLIRQAIASAINVDEIIEGSGGINKRNPWMSFPDTP
                     YYLGEAAPTPWYDIKDLAKSRELLKQAGYNNEKIIIETNSNYQWMRSTMLVVAEELKA
                     AGINVDVQVTDWTTNAAHMQQGTGDWNLSTTAFGPDHVLG"
     gene            complement(1346..2506)
                     /locus_tag="VN968_15885"
     CDS             complement(1346..2506)
                     /locus_tag="VN968_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXL30751.1"
                     /translation="MYGHMRAAGLPAGYPQFFARAEGCYLWDVDGRKYLDFMCSWGPV
                     VLGHRHPHVDEAARRQMEQGDCLNGPTERMVELAERLVGLVTHADWAMFSKNGTDATT
                     TCVTLARAATGKRKILLASGAYHGAVPWCSPYPYGVTAEDRAHAIYFKYNDIDSLEAA
                     AGQAGSDLAGILVSAFRHDYGQDQELPDPAFAARARALADANEAALILDDVRAGFRLT
                     LHGSWDQIGVQPDLSAWSKAIANGYALAAVAGNERFRQAASQLYATGSFWCGATSMAA
                     ALATIDVLQAENGPEHMRRMGQRLRDGIAAQAARHGVGVRQSGPPQMPTILFEDDADY
                     AKGARFVLEALKRGVYLHPKHNMFLSLAHTAADIDAALAVTDEAFGVVAKLQ"
     gene            complement(2708..5086)
                     /locus_tag="VN968_15890"
     CDS             complement(2708..5086)
                     /locus_tag="VN968_15890"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HXL30752.1"
                     /translation="MTVTSKNPNPADHLPDMESPAAVPGLLNAVDVRRDSWGIPHIRA
                     ASQLDAFAGLGFVHAQDRLWQMEALLRRGTGRYAEWLGKPALAGDILARRVDTTGASR
                     RDFAILKSETRSMLEAYARGVNAFIAVRRWPAEYAILKTEPAPWEPWHSIAVMRQIGF
                     LMGSVWWKLWRAAALPIVGVAEISKLRFDDGGDDLLCIPPGAEGARYLASLGDLKPGL
                     EALIGHGSDQQEAELAGGSNNWALSPDRTATGRPLLAGDPHRVLEMPNMYAQTHLACD
                     EFDIVGLTVPGVPGFPHYGHTANVAWCVTHAFVDIHDLYIERFDAGAENTRFKGDWTP
                     VKRRKESVSVRSEKDAEIEVIETRHGPVIAGDPAAGTALALRSVQFAVPDKSFDCMLP
                     MLRAANVEQLYEATRDWGLMDHNLVAADTAGHIGNRVRARVPARPKSNGWLPVPGWTG
                     EHEWNGMIPFEEMPCCIDPEGGTIVTANNRVMENGKRYLSTDSMPPHRARRIWQRLAQ
                     LSRAGVDDMASVHRDLVSVVALEFRDRIRDIKVLGHAAELRDRIINWNGEMTAESHSA
                     VAYVALRTGLTKLVAEKSGLSAVSESPYAKVTPGIFGESQIWWTVPQLLRANDTGLFG
                     PATWDELLAQALERAALNFPPESFPSETFQSETFQSKAFQSWAELHRPTLRHPLSSAF
                     PEFAGMLDKPCVAVGGDNDTVFATGYVARLGMRATYGSLCRYVFDIGAWDNCKWIVFH
                     GSSGHTASPWYNNQNATWAAGEMVPMLYDWTKIEQLATTHQKLMPVEAPRAA"
     gene            complement(5143..6597)
                     /locus_tag="VN968_15895"
     CDS             complement(5143..6597)
                     /locus_tag="VN968_15895"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXL30753.1"
                     /translation="MFRETWRSNLTSRKLFWIARLAALVSILPATSGRATAEALNPQQ
                     QLAFDIYKELVEINTVTATGDTAQAAGAMAARLRAAGFAEADVHVFQPVPRKGNLVAR
                     LHGTGARKPILLVAHLDVVPASREDWSVEPFKLTEQDGYFYARGSGDDKYMAAAFVTN
                     LIRYKQEGYKPDRDIILALETDEEIFDSDALGIQWLIRHHRDLIDAEFALNEGGGVGL
                     KDGKPIRNSVQTSEKVVLNYQLDVKNKGGHSAVPLKDNAIYHLAEGLVRLSKFSFPLK
                     LNETTRAYFERTAQFEGEQTAADMRALLSDKPDPAAMTFVRLAANPVYNAQLRTTCVA
                     TMLEGGQAINALPQSASAKVNCRAMPGEPVDEVKATLERVLADDQITVTQLGSPVLSA
                     PSALNEEVMGSIEKLSQQFWPGAVVLPVMSAGATDGSFLRNAGIPTYGHSGLASDVND
                     GGRIHGKDERVLVKSFYQGEDYLYRLVKMLSGGG"
     gene            complement(6693..7196)
                     /locus_tag="VN968_15900"
     CDS             complement(6693..7196)
                     /locus_tag="VN968_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXL30754.1"
                     /translation="MKPAAIPTLGALLAGALIASLNAARADVVRYPIPNSTFPISQAV
                     QVPGNATTYYISGQVPPVLSKEADPASPQAYGDTKTQTVGVLNKIKTILEGFGLGMAD
                     VVKMQVFLVHDARAPMDFKAFMEGYTQFFGGSQPNLPARSVVGVAALANPGFLVEIEV
                     VAVKDNK"
     gene            complement(7229..7705)
                     /locus_tag="VN968_15905"
     CDS             complement(7229..7705)
                     /locus_tag="VN968_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXL30755.1"
                     /translation="MRRLQNRIPRAFVPMTPGNTNCHAAFVMTISAALLLSSFMACAQ
                     SAEPPASRPALSTGFRFIENSGEELFANVCRGCHMPDGKGAVGAGTYPSLVANKTLEA
                     GGYPVDLVVNGQRAMPPFGAMMSDDQVAAVVNYLRTHFDNHYQDAVTAADVQTVRR"
     gene            complement(7705..9300)
                     /locus_tag="VN968_15910"
     CDS             complement(7705..9300)
                     /locus_tag="VN968_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL30756.1"
                     /translation="MSSEPGTVSRRDVLSLIGAVSGSAAMYHAMTSLGFAADSGYKGP
                     IKLDGDPKGASVLILGAGLAGMTAALELRKAGYNVRILEFNSRAGGRNWTLRGGDSFT
                     ELGGFRQTCEFEQGLYINPGPWRIPYHHRALLDYCKRLGVVLEPFIQLNHNALLHTTR
                     AFGGAPQRIRDIKTDFQGQISELLAKATQQGRLDEAVSKEDREILMQALRSWGALDGN
                     YAYKANLISADFRGYAKDPGGGVGAAPIAGEPINLSDILKSRVWRYLQNFALHTFQTT
                     MFQPVGGMDMIGKAFAREVGDVIRYEAKVTAIQQDDHGVTVTYTDLKAPATPQQAKAD
                     WCVCTIPLSILSQLPIDVNDRMKAAIDAVPYSPSVKVGLQFKRRFWEEDEAIYGGISY
                     TDLPIRQIAYPNTGFNSSAKGVLLGAYLFDGPNAYEFTAMSPAERIARAVEFGAGIHP
                     QYRTEYENGVAVAWHRMPFTLGCAGNWTEKARAEHYDDLCQIDGRIVLAGEHASYIPA
                     WQEGAILSSLNAITRLHDRVVKT"
     gene            9641..10993
                     /locus_tag="VN968_15915"
     CDS             9641..10993
                     /locus_tag="VN968_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXL30757.1"
                     /translation="MADPNVRSAGHSGSGVLELLRLFKGSAAAADQYAATCLEQAKTI
                     EPVLRAFEYFAEDVARKPGPLSGIPVGIKDIIATADMPTTNGSPVYKDCIPDADAWVV
                     ERLRNLGATIFGKTVSTEFAWRHPGPTVNPWNPKHTPGGSSSGSAAAVAAGLVPLALG
                     TQTLGSVIRPAAFNGVVGLKPSFGAIPRTGVHPLSPSLDHVGFFARRVDDVAYALSLL
                     GGTSDSDVHGRPLPAFQVSVEQGLPPLAAPRLAVVRFAKWSKAEPEQQQLFDTAVAAL
                     RDAGAIVEELELPELDHANWSAINTILACEAALIFGDLVERFPDRTSDRLKELVRTGK
                     AHIAYDYLAAKAMQEKLRSAFSAQMSGFDAVLTLPAFGEAPEGLGYTGDAEYCAPWTL
                     LGVPAVSLPAGFGKNGLPLGLQLVGAYREDYWMLRVAKWIEGVLMVDMGIPRIDRTEV
                     "
     gene            11085..13823
                     /locus_tag="VN968_15920"
     CDS             11085..13823
                     /locus_tag="VN968_15920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HXL30758.1"
                     /translation="MIAARPQRQTFTKDSPGMARAVRHLAAALAATVVLALLLSGNAV
                     AEPGYSFDTTPGKLPKAVIPLHYAIELTPDLASLQLEGVEAVDIEVREPAARLTLNAV
                     NITLGAATVDDDAQRADVALDAATETATLTFPKALAAGPHRLRIGFTARINAFGRGLF
                     FVDYPTDNGVKRMLSSQLEPADARRIFPCWDEPAFKATFALTVTVPRTFLAVSNMPVA
                     REEPVAPDKKQVAFAPTPKMSSYLFVFAAGELERLTAETDGVTVGVVTTAGKREQGRF
                     ALENAVKLLDYYNDYFGIKYPLPKLDLIAVPGGFGGAMENWGGITFFESRLLFDPSTN
                     PDTARRGIFSVLAHEMAHQWFGDLVTMGWWDNLWLNEGFASWMQEKAAEHFYPQWQTW
                     LNGYGQKQYAMALDARRTSHPIQQPVADHSEANAAFDGITYNKGQALIRMLENHLGER
                     AFRSGIRKYMAAHAYGNTTTADLWQTLESAAGKPVTGIAASFTEQDGVPLIVAETSCS
                     GDMQRMTLRQDRFVIIPAASSAASTQATVLPPRTWQVPISVGPLRATQPAEHLLLAGS
                     SDIAAGSCGEAVKVNLGDVGYYRTEYGPNSRAALAKSLALMTPDDRVNFLADSWALVQ
                     AGRAEPPSYLALIEQISRDDRRAVWDQAIGSLTTLDRLARDRAERPALQGYARARLRP
                     PFDRLGWDGSGSGDDENTLLRASLISALGQFGDEDILAEARRRFAAFVQNPQSLPNAL
                     RDPVTHLVGISADRASYDTLLALARKSTVTNERLRYYYAAASARDATLARATLALTLT
                     DELPNTIVGGLINTVASSGEQPDLAWEFVQTNFDALSAKQGPSFRDAFVANFMTNFTD
                     DAHAAELSHFAPAQATSGGRVITARALETIAISADLKTRALPAVNAWIKEHNGGRP"
     gene            complement(13878..14852)
                     /locus_tag="VN968_15925"
     CDS             complement(13878..14852)
                     /locus_tag="VN968_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HXL30759.1"
                     /translation="MTHAIRFHQTGGPEVLVWEEVKPGKPGPGEARIRHTAVGLNFVD
                     IYNRSGLYPTEVPSGLGSEGAGVVEEVGPGVSDLKPGDRVAYGNGPLGAYAEARLIPA
                     DRLLKLPDGIDDKTAAAMMLKGLTVQYLIRQTYRVKAGDTILLHAAAGGVGLILGQWA
                     KHLGATVIGTVGSDEKAKLAKAHGCAHTVVYTREDFVKRVGEITEGKKVPVVYDSVGK
                     DTFLKSLDCLAPLGVVALFGQSSGSVEPLNLGLLAQKGSLYVTRPTLNTYGARRENLV
                     AMAKELFEVVLSGAVKIEVNQTYPLKDAAKAHADLAARKTTGSTVLTV"
     gene            complement(14936..17170)
                     /locus_tag="VN968_15930"
     CDS             complement(14936..17170)
                     /locus_tag="VN968_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL30760.1"
                     /translation="MNASVILDRRRVLAGGGALIVSFSLTAAFAQDQTAPAAAPVQNP
                     PGSLKGAPFLDSWIRIDADGSITAFTGKTELGQGFKTAFQQIAAEELDVPFESLKVVT
                     ADTRLTANEGYTSGSHSMQDSGTAIQNAAAQVRELLVEEAARRLGLPAENFRTENGAV
                     ISSGGRRFAYGDLVAADMLHVQAQPKSKLKDPATFKVMGQPIPRVDIPAKVTGGAAYV
                     QDMRLPGMVHARIVRPPSYGAQLTDCDTSAIEKLPGVVKVIRDGNFLAVVAAKEFLAV
                     KAMKALSAAATWKETAGLPKQDDLLHLLTSLPSQDSTIFQQNNPSAVGQKTIEATYTR
                     PYQAHGSIGPSCAVAQWVDGAMTVWTHTQGVYPDRQGIAEMLRVPPASVRCIHVEGSG
                     CYGHNGADDAAADAALIARALPGTPVRVQWMREQEHIWEPFGPAMATKLKASLDDNGA
                     IADWNFEVWSNTHSMRPGGAGSMLAAQHMSQPFAVPAPKPLPLPEGGGDRNAIPIYTF
                     PNAHVVHHFIPAMPLRISAMRSLGAYHNVFSIESFMDELAGAAGADPVEFRLKHLNDP
                     RGREVIEKAARGFGWLKGQQAPQDRGFGFAFARYKNLAAYCAIATEVEVNRETGRLRL
                     VRAVAAVDSGQVVNPDGLINQIEGAIVQSMSWTLYESVTFDDTRITSIDWQTYPILRF
                     NSVPESIEVHIINRPGKPFLGSGETGQGPAAASIANAIADATGKRLRDLPLTRKRIKD
                     AIDA"
     gene            complement(17191..17688)
                     /locus_tag="VN968_15935"
     CDS             complement(17191..17688)
                     /locus_tag="VN968_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL30761.1"
                     /translation="MMTLKVNGVDHQINADPDTPLLYVLRDDIKLNAAKFGCGLGQCG
                     SCTVIVDGKAVLSCVTPLLLLEGKQVTTLEGLGTVEAPAPIQRAFMEEQAAQCGYCIA
                     GMMMRAQALLQRNPKPTDEQIRSELEPHLCRCGTHMRILRAVHRASRLMDTADASSAS
                     QRSAP"
     gene            complement(17685..19046)
                     /locus_tag="VN968_15940"
     CDS             complement(17685..19046)
                     /locus_tag="VN968_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXL30762.1"
                     /translation="MRKSRIIGSVIAALLMAGAVAAFAIAWRPAIAAIDPPAPQSFDA
                     ALVKRGRDLVAIGNCNDCHTVRGGKNFAGGLPVPTPFGTIFSSNITPDAETGIGQWSE
                     AAFRRAMQSGVDRDGQHLYPTFPYDHFTNVTGEDDQALYAYLMTRQPVHAAARANQLS
                     FPLDQRFVIAGWKLLFLRHGTFQPDLAQSAEWNRGAYLVEGLAHCGSCHTPRNALGAE
                     RATAQFAGGDVDNWHAYAINAQSPAPVPWDADALFAYLRDGWHPDHGVARGPMAQVVS
                     NLSSVPPSDIRAIATYMAGVFGAPTPDRKRQGEAVLAQVRSPSAQAPKGDAAGAAIYA
                     AACATCHETGRTLPYGGVNLALSTAIAGPDPRNVANIVLSGLRPVEGERSPIMPGFAG
                     SMNDGQITALLNYLRARFSNQPAWTDVEKTVEDARRTQIAFLRISPGPHNAPADPTQR
                     DKP"
     gene            complement(19162..19479)
                     /locus_tag="VN968_15945"
                     /pseudo
     CDS             complement(19162..19479)
                     /locus_tag="VN968_15945"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19498..19806)
                     /locus_tag="VN968_15950"
     CDS             complement(19498..19806)
                     /locus_tag="VN968_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30763.1"
                     /translation="MCAGDHDGAGRALRLRACADFAEDSDVAQAGCDVEPCPLYCCNA
                     LGRHTSAGAASGQTADVTRVVVRGNPLLSDRTDRPDISTGRESQVSVHHAVAAALVTG
                     "
     gene            20040..>20786
                     /locus_tag="VN968_15955"
     CDS             20040..>20786
                     /locus_tag="VN968_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HXL30764.1"
                     /translation="MLHRSVTTAHVCGVGIFLSSLLSGGAYAADLLTKAPPVPYAATD
                     DFWTRPYLLGDLGRTRLKEMGIDLSLQLGDEAVTNVTGGTNKVATQAGQLTFQGKFDM
                     AKLAGIPGGLIGITLVDRFGHNLNTEAGIPALQLTNEVFGRGNILRLTELYYSQKLFD
                     DRLELKGGRLPVGSDFFFGQCEFINLTFCGGQPGNIQGGYIFNFPVSQWAGLAHFNIT
                     KEVQISAGVYDANPNYLTTSDMGVYLAPGIP"
BASE COUNT         3845 a         6617 c         6383 g         3941 t
ORIGIN      
        1 cccagaacat gatctggccc gaaggccgtg gtcgacaggt tccagtcgcc ggtgccttgc
       61 tgcatatgcg cggcgttggt ggtccaatcg gtgacctgaa cgtcgacgtt gattccggcg
      121 gccttgagct cttcggcgac aaccagcatg gtggatcgca tccactggta attgctgttg
      181 gtctcgataa tgatcttctc gttgttgtat ccggcctgct tgagaagttc cctggatttc
      241 gcaaggtcct tgatatcgta ccagggggtc ggcgcggcct cacccagata atacggcgtg
      301 tcgggaaacg acatccacgg attgcgtttg ttgatgccgc ccgagccctc gatgatttcg
      361 tcgacattaa tggctgacgc gatggcctgg cgaatcagaa cattggccgt caaacccttt
      421 tgcgagttga cgatgatgta acttccgagc agcgggaaga gctcgcggac cgcaagatcc
      481 ttgcgttccg tgatgcgggc gctgagttca tttggaatcg tcgaaatcaa ctgcgcttcg
      541 ccattctgca gcgcggcgat ccgggtggtc gcttccggca tgaaacgata gcgtaccgca
      601 tcgaggtaaa ccgtcttgcg tccgccgtaa ccgtcgcgcc caggggcact ggcgtcggcc
      661 acgtaatcgt cgaagcgctt gatgacaagg tggctgtcct tgacccactc gcccatcatg
      721 tacggcccgg tgccgatgag atcgacgccg cgcgcgggct tgtccttctg cgactccggc
      781 aagatcatga gcggataaat cggtgatttc agaacgtcga gaaggacgac gtttggctgc
      841 ctcaatacga tgacaaaact gtagggatcg ggcgtatcga aatgatcgac gccttcgaga
      901 ttttttgcgt tcggactgac gcgccgatag cgctcgaacg atgccttgac gtcggccgag
      961 gtcatctcct ggccgttatg gaatttcacg cctttgcgaa gttcgaacga gtaggtcttg
     1021 aaatcctccg aagccatcgc cttcgacgcc agcatggtgc gcgtcgcatt gttggcgtcc
     1081 agcgtgacca gtccttcaaa cacgttgttg atgacttcga gctcgaccga gctggaggcg
     1141 acatagggat cgagtgtgcc gggtccggag acgctggcat agaccagcgt gcctcccttc
     1201 tttggctgct gcgcaaagct gacgccgctc gtggaaagat ggactgaaag cgccaggacg
     1261 agcccggaaa gacatcgtgc aacagatcgc ctcattcgct tcaattcctc gatttatgcc
     1321 gcaaggcctc gatcaccgcg agcagctatt gcaatttcgc tacaaccccg aacgcctcat
     1381 ccgtgaccgc cagggcggca tcgatatccg ccgcggtgtg ggcgagcgat aggaacatat
     1441 tgtgcttggg atgcaggtag acgccgcgct tgagcgcctc cagcacgaag cgggcgcctt
     1501 tggcataatc ggcatcgtct tcgaacagga tcgtcggcat ttgcggcgga ccgctttgcc
     1561 ggacaccgac gccgtgacgg gccgcttgag ctgcgatgcc gtcgcgcaat cgctgaccca
     1621 tccgccgcat atgctccggg ccgttctccg cctgcagcac gtcaatggtc gccagcgcag
     1681 ccgccatcga cgtcgcgcca caccagaatg aaccggtcgc ataaagctgg gacgcggcct
     1741 gccggaagcg ctcgttgccg gcaaccgctg cgagcgcgta accgttggcg atcgccttgc
     1801 tccacgcact gagatccggc tgcacgccga tctggtccca gctgccatgt aatgtcaggc
     1861 gaaagcccgc cctgacgtcg tcgaggatca gcgcggcttc gttggcatcg gcgagcgcgc
     1921 gggcgcgcgc ggcgaaggcg ggatcaggca attcctgatc ctggccgtag tcgtgacgaa
     1981 acgccgacac caggatgccg gccagatcgc tgccggcctg ccccgctgcg gcttcaaggc
     2041 tgtcgatgtc attatatttg aaatagatcg catgggcgcg atcttcggcc gtcacgccat
     2101 atggataggg cgagcaccac ggcacggcgc cgtgataggc gccggaagcc agcagaatct
     2161 tgcgcttgcc ggtcgcggcg cgcgccagcg tgacgcaggt cgtggtggcg tcggtgccgt
     2221 tcttggaaaa catcgcccaa tcggcatgcg tcaccaatcc gaccagtcgc tccgccagct
     2281 cgaccatgcg ctcggtagga ccattcaggc agtcgccctg ctccatctgc cggcgcgcgg
     2341 cctcatcgac gtggggatga cgatgcccga ggacgaccgg accccagctg cacatgaaat
     2401 cgagatattt gcggccgtcg acgtcccaga ggtaacagcc ctcggcgcgc gcgaaaaact
     2461 gcggatagcc cgcaggcaag ccggctgccc gcatatgtcc gtacatgccg ccgggcacga
     2521 cctgaagagc gcgtcgacgc aggctttggt ccagacccgc gatcctgctt tcagtcaaag
     2581 ccaacttctc cggccaagtt gcaaaaagga tatctcatgc ggcaacagac acaaccgctt
     2641 aatccacggc tcggcaacga gcgccacgca taacgctctg ccgatgactc attcgatcga
     2701 cgccatttta ggcagcgcgc ggagcctcaa cgggcattag cttctgatgt gtcgtggcca
     2761 gctgctcgat tttcgtccaa tcgtaaagca tcggcaccat ctcgccggct gcccaggttg
     2821 cattctggtt gttgtaccat gggctcgcgg tgtgacccga cgatccatgg aagacaatcc
     2881 atttgcaatt gtcccacgct ccgatgtcga acacgtagcg gcacagcgat ccataggttg
     2941 cgcgcatgcc gagccttgcg acgtagccgg ttgcaaatac cgtatcgttg tcaccgccga
     3001 cggcaacgca gggcttgtcg agcatgccag cgaattccgg aaacgccgat gacagcggat
     3061 gacgcaaggt gggccgatgc agctcggccc aagattgaaa agccttggat tgaaaagtct
     3121 cggattgaaa agtctcagat ggaaaactct cgggcggaaa attcagcgcg gcgcgctcga
     3181 gcgcttgcgc caaaagctca tcccaggtcg ccggcccgaa cagtccggtg tcgttggcgc
     3241 ggagcagttg aggcacggtc caccagatct ggctctctcc aaaaatcccg ggcgtgactt
     3301 tcgcataggg gctctcggag accgcactca gtccgctttt ctcggcaacc agtttggtca
     3361 gaccggtccg cagcgcgaca taggccacgg cgctatgcga ctcggcggtc atttcgccgt
     3421 tccagttgat gatgcggtcg cgcagttcgg cggcgtggcc caagaccttg atgtcgcgga
     3481 tacgatcgcg aaattcgagc gccaccacgc tgacgagatc gcgatggaca gaggccatgt
     3541 catccactcc ggcccttgaa agctgcgcaa gccgctgcca gattcggcgg gcgcgatgcg
     3601 gcggcatgct gtcggtcgac aggtagcgct tgccgttttc catcacacga ttgttggccg
     3661 tcacgatcgt tccgccctcg gggtcgatac agcatggcat ttcttcaaac ggaatcatgc
     3721 cattccattc gtgctcgccg gtccagcccg gcaccggcag ccagccgttc gacttcggac
     3781 gcgccggcac cctggcgcgc acgcgattgc cgatatgtcc tgcggtatcg gccgcgacga
     3841 gattgtggtc catcaaaccc cagtcgcggg tcgcctcgta gagttgctcg acattcgccg
     3901 cccgcagcat cggcagcatg caatcgaagg atttgtccgg aacggcaaac tgcactgaac
     3961 gcagcgccag cgccgtgccg gcggccggat cgccggcgat taccggccca tgacgggtct
     4021 cgatgacttc gatctcggca tccttttcgc tgcgcaccga aacgctctcc ttgcggcgct
     4081 tgaccggcgt ccagtcacct ttgaagcgcg tattctctgc gccagcgtca aagcgctcga
     4141 tatagaggtc gtgaatgtcg acgaacgcat gcgtcacgca ccaggcgacg ttggccgtgt
     4201 gaccgtaatg cggaaatccc ggtacgcccg gcaccgtcag accgacaatg tcgaactcat
     4261 cacaggcaag atgcgtctgc gcatacatgt tgggcatttc cagaacccgg tgtgggtctc
     4321 ccgcgagcaa cggacggccg gttgcggtgc ggtccgggga gagagcccag ttattgctgc
     4381 cgccggcgag ttccgcctcc tgctgatcac tgccgtgacc gatcaatgcc tcgagtcccg
     4441 gtttcagatc gccgagcgag gcaagatagc gggcaccttc tgcgcccggc ggaatgcaaa
     4501 gcaggtcgtc acccccgtca tcaaaacgca gctttgagat ctcggcaacg ccgacaatgg
     4561 ggagtgcggc ggcgcgccag agcttccacc acaccgagcc catcagaaac ccgatctgac
     4621 gcatcaccgc gatcgaatgc cagggctccc atggcgcggg ctcggttttg agaatggcat
     4681 actcggccgg ccatcggcgc acggcaatga aggcattgac cccgcgcgcg taagcctcca
     4741 gcatcgatcg cgtctcggac ttcaagatgg cgaagtcgcg ccgcgatgcc cctgtcgtat
     4801 cgactcgccg cgccagaata tcgccggcca gcgccggttt cccgagccat tccgcgtaac
     4861 gtccggtgcc acgccgcaac agcgcttcca tctgccatag ccggtcctgc gcatgaacga
     4921 acccgagccc ggcaaaggca tcaagctgcg aagccgcgcg aatatgggga attccccaac
     4981 tatcccgtcg cacatcgacc gcgttcaaca aacccggcac cgccgccggg ctttccatat
     5041 cgggaaggtg gtccgcggga tttggatttt tcgaggttac cgtcatgggg ccttctgatg
     5101 tgtccctgct tgcctgcgat ggcgaggtag aattcttcct tgctagccgc cgccggacag
     5161 catcttgacc agccgataga gatagtcctc gccctggtag aacgatttca ccagcacccg
     5221 ttcgtccttg ccgtggatcc ggccgccgtc gttgacatcg ctggcgagcc cggagtggcc
     5281 ataggtcgga atgccggcgt tgcgcaggaa gcttccatcc gtggcgccgg cgctcatgac
     5341 cgggagcacg accgcgccgg gccagaactg ctgggataat ttctcgatcg accccatcac
     5401 ctcctcattc agcgctgatg gcgcgctcag gaccggcgaa ccgagttggg tgaccgttat
     5461 ctgatcgtcg gccagtacgc gctcgagcgt cgccttgacc tcgtcgacgg gctcgccggg
     5521 catggcccgg caattgacct tggcgcttgc cgactgcggc aacgcgttga tcgcctgccc
     5581 gccctccagc atcgtcgcca cgcaggtggt gcgcagctga gcgttataga ccggattggc
     5641 ggcgagccgg acgaacgtca tcgcggccgg atccggttta tcggacagca gcgcgcgcat
     5701 atcggccgcg gtctgttcac cctcgaattg cgctgtccgc tcgaagtagg cgcgcgtggt
     5761 ctcgttgagc ttcaagggaa agctgaattt ggagagacgc accagtcctt ccgccagatg
     5821 atagatcgca ttgtccttca acgggaccgc gctgtggccg cccttgtttt tcacgtcgag
     5881 ctgataattc agcacgacct tctcgctggt ctggacgctg ttccggatcg gcttgccgtc
     5941 cttgaggccg acgccgccgc cctcgttcaa tgcgaattca gcatcgatca ggtcgcgatg
     6001 atgcctgatc agccactgga ttcccagcgc gtcactgtca aagatttcct cgtcggtctc
     6061 gagcgccaga atgatgtcgc gatcgggctt gtagccttcc tgcttgtagc gaatgaggtt
     6121 ggtgacaaag gccgccgcca tgtatttgtc gtcgccactg ccgcgcgcat agaaatagcc
     6181 gtcctgctcg gtcagcttga acggctcgac cgaccagtcc tcgcggctgg ccggcacgac
     6241 gtcgagatgc gccaccagca ggatcggttt gcgcgcgccg gtgccgtgca ggcgcgcgac
     6301 cagattgccc ttgcgcggca ccggctggaa tacgtggaca tcggcctccg caaaacccgc
     6361 ggcgcgcagc cgggccgcca tcgccccggc cgcttgcgcg gtgtcgccgg tcgcggtcac
     6421 ggtgttgatt tcgacaagct ccttgtagat gtcgaaggcg agctgctgtt gcgggttcag
     6481 cgcttcggcg gtcgcccgcc ccgacgtcgc ggggaggatc gagaccaggg cggcgaggcg
     6541 agcgatccag aaaagcttcc gcgatgtgag gttgctacgc caggtttctc gaaacatccc
     6601 gctctcccaa aaaaagcagc cggatgaatt cagacaggta acgcgttcgc tggcaagatg
     6661 tcggaccaga tagttctgcg gctcagctat ccctatttgt tatccttgac agcgaccacc
     6721 tcgatttcga ccagaaaacc gggattggcg agtgctgcga cgccgacaac cgaccgtgcc
     6781 ggcagattgg gctgactgcc gccaaagaac tgcgtgtagc cctccatgaa tgccttgaaa
     6841 tccatcggcg cgcgcgcgtc gtgcacaaga aacacctgca tcttgaccac gtcggccatg
     6901 ccgagcccaa aaccttcgag gatggtcttg atcttgttga gaacaccgac cgtctgggtc
     6961 ttggtgtcgc cataggcctg agggctggcc gggtcggctt ccttgctaag gacgggaggc
     7021 acctggccgc tgatataata ggtcgtcgcg ttgcccggga cctgtacggc ctgggaaatc
     7081 ggaaaggtcg agttcggaat gggatatctg accacgtcgg cccgcgcggc gttgaggctg
     7141 gcaatgagag cgccggccaa aagcgctccg agtgtcggta ttgccgccgg cttcatctgc
     7201 ccctccgcgt gttggatgag aatggtcgtt atcggcgaac agtttgcaca tccgcggccg
     7261 tcaccgcatc ctgataatga ttgtcgaaat gggtccgcag gtaattcacc acggcggcga
     7321 cctgatcgtc gctcatcatc gctccgaacg gtggcattgc gcgctgtccg ttgacgacga
     7381 ggtcgacggg atacccgccg gcctcgagag tcttgttggc caccagcgat ggataggttc
     7441 cggcgcctac cgcccccttg ccgtcgggca tgtggcagcc ccgacagaca ttggcgaaca
     7501 actcctcgcc gctgttttca atgaacctga agccggtact cagggctggc ctggatgccg
     7561 ggggctcggc gctctgcgcg caagccataa acgatgatag caagagtgct gccgatatcg
     7621 tcatgacaaa cgctgcatga caattggtgt tgcccggcgt catcgggaca aacgcccgcg
     7681 gaatcctgtt ttgaagccgt cgcatcacgt cttcaccacc cggtcatgca gccgggtgat
     7741 ggcgttgaga gacgacagaa tggcgccctc ctgccatgcc ggaatgtagg atgcatgctc
     7801 gcccgccaac acgatcctgc cgtcgatctg gcagagatcg tcgtagtgct ccgcccttgc
     7861 cttctcggtc cagttgccgg cgcagcccaa cgtaaacggc atgcggtgcc aggcaacggc
     7921 aacgccattc tcgtattccg tgcggtattg cggatgaatg ccggcgccga attcgacagc
     7981 ccgcgcgatc cgctcggctg gcgacatcgc ggtgaattcg taggcgttgg gaccgtcgaa
     8041 aagatacgcg cccagcagca cacccttggc cgagctgttg aagccggtgt tgggataggc
     8101 gatctgacgg atcggcaggt cggtgtagct gatgccgccg tagatcgcct cgtcctcttc
     8161 ccagaagcgt cgcttgaact gcaggccgac cttgaccgac ggcgaatagg gcacggcgtc
     8221 gatggctgcc ttcatccgat cgttcacatc gatcggaagc tggctcagaa tcgagagcgg
     8281 aatggtgcag acgcaccagt cggccttggc ctgttgcggc gtcgccggcg ccttgaggtc
     8341 ggtgtaggtg accgtgacgc cgtggtcatc ctgctggatc gccgtcacct tcgcctcgta
     8401 acgaatgacg tcgccgacct ccctggcaaa cgccttgccg atcatatcca tgcctccgac
     8461 cggctgaaac atcgtggtct ggaaggtatg caacgcgaaa ttctgcagat agcgccagac
     8521 ccgcgatttg aggatatcag acagattgat cggctcgccg gcgatcggtg ccgcgccgac
     8581 gccgccgccc ggatccttgg cgtaaccgcg aaagtcagcg gaaatgaggt tggccttgta
     8641 ggcgtagttg ccgtccagcg cgccccacga cctcagcgcc tgcatcagga tctcgcgatc
     8701 ctctttcgag acagcttcgt ccagtctgcc ctgctgcgtt gccttggcga gcagttccga
     8761 aatctgtccc tggaagtcgg tcttgatgtc gcgaatgcgt tgcggcgcgc cgccgaatgc
     8821 cctggtcgtg tgcagcaggg cgttgtgatt aagctggatg aacggctcga gcacaacacc
     8881 gaggcgcttg cagtaatcga gcaacgcgcg atggtgatag ggaatccgcc aaggcccggg
     8941 attgatatag aggccttgct cgaattcgca ggtttgcctg aagccgccga gttcggtgaa
     9001 cgaatccccg ccccgcaggg tccagttgcg gccccccgcc cggctgttga attccaggat
     9061 ccggacattg tatccggcct tgcgcaactc cagcgcagcc gtcatgccgg caaggccggc
     9121 gccgaggatg agaacggaag cgcccttcgg atcgccgtcg agcttgatcg gacctttata
     9181 tccggaatcg gccgcaaacc ccaggctggt catcgcgtga tacatcgcgg cgctgccgga
     9241 aaccgctccg atcagagaca gcacatcacg ccggctaacg gttccaggct cgcttgacat
     9301 cgctactcat tacccgcgat ttcgcccggc cagtcatcaa atccaggtgt cgggactccg
     9361 acgacaaatt cgacgcgccg ctcccgatcg gaagtgcctg ctttaaattc gacgtgtatc
     9421 atgaccttgc gtaaacttcg cggcgggcgg gtcacaccgg cctaaagcgc acggaactcc
     9481 tttcaacgcg atgaaaataa aaggtttttt atgggtgagc tgatgccacg caatcgcggc
     9541 aaccatcgaa ccggggcggc gacgaagcaa gcttgcgaat gagacaacgc ggcggccgac
     9601 cgccgcgtcc gtctgtcttc tcatgcggga ctattccgac atggctgatc cgaatgtgcg
     9661 ctctgccggc cattccggct ccggcgttct cgaattgttg cgcctgttca aaggcagtgc
     9721 tgccgcggca gatcaatacg ctgccacctg cctcgagcag gccaaaacta tcgagccggt
     9781 gctgagggca ttcgaatatt ttgccgagga tgttgcgcgc aagccgggac cgctgtcggg
     9841 cattcccgtg ggcatcaagg acatcatcgc gacggcggac atgccaacga caaacggctc
     9901 gccggtctac aaggattgca ttcccgacgc cgatgcgtgg gtcgtcgaac ggctgcgcaa
     9961 ccttggcgcc acgatcttcg gcaaaacggt ttcgaccgaa tttgcatggc gccatcccgg
    10021 accgacggtc aatccatgga atccaaaaca cacgccgggc ggttcgtcat ccggttcggc
    10081 ggcagctgtc gcggctgggc tagttccgct ggcgctgggc acgcaaacac tgggctcggt
    10141 catcaggccg gcggcgttca atggtgtcgt cggcctcaaa cccagtttcg gcgcgatccc
    10201 gcgcaccggc gtgcatccct taagcccttc gctcgatcat gtcggatttt tcgcgcgccg
    10261 tgtggacgat gttgcctatg ccttgtctct ccttggcggc accagcgaca gtgatgttca
    10321 tggccggccg ctccccgcat ttcaggtttc cgtcgaacag ggattgccgc cgctggcggc
    10381 gccccgtctc gccgtcgtgc ggtttgcaaa atggtcgaag gccgaacccg aacagcaaca
    10441 gctgttcgac accgcagtgg cagcattgcg cgacgccggc gccatcgtgg aggaactgga
    10501 attaccggaa ctcgatcacg ccaactggag cgcaatcaac acgatcctag cctgcgaggc
    10561 cgcgttgatt tttggcgacc tcgtcgaacg ctttcccgac cgcaccagcg atcgcttgaa
    10621 ggagctcgtt cgaaccggca aagcccatat cgcctacgac tacctcgccg ccaaagcgat
    10681 gcaggaaaag ctgcgatcgg cattctccgc gcaaatgtca ggcttcgatg ccgtgctgac
    10741 gctgccggcg ttcggcgaag cgcccgaagg cctgggctat accggcgatg cggaatattg
    10801 cgcgccctgg accttgctgg gcgtgcccgc cgtgtcgctg cccgccggat tcggtaagaa
    10861 cggtcttccg ctgggcctcc agctcgtcgg cgcctatcgg gaagattatt ggatgcttcg
    10921 tgtggcgaaa tggatcgagg gcgtcttgat ggttgacatg ggcattccga ggattgatcg
    10981 cacggaagtt tgaacgctgc ggcggacgcc tttttcctcg caatctccat cccgttggga
    11041 taactcacgg cggccgttaa aaccacgcat gcaaggccga gccgatgatc gccgcgcgcc
    11101 cgcaaagaca aacattcacc aaggattcgc ccgggatggc gcgcgcggtg cgccacctcg
    11161 ccgccgcgct ggccgcgacc gtcgtgcttg ccttgctgct gtccggcaac gccgtcgccg
    11221 agcctggcta ttccttcgac accacgccgg gcaagctgcc gaaagcggtt atccccctcc
    11281 actatgcgat cgagctcacg ccggatctcg cgagcctcca gcttgaaggt gtcgaggccg
    11341 tggatatcga ggtgcgcgag cccgccgcac ggcttacgct caatgcggtc aacatcacgc
    11401 tcggcgccgc caccgtcgac gatgacgcgc agcgcgctga cgtcgcgctc gatgccgcga
    11461 ccgagaccgc gacgctcacc tttccgaaag cgctcgcggc gggcccacac cggctgcgca
    11521 tcggtttcac ggcacggatc aacgcgttcg gccgtggtct tttcttcgtc gattacccga
    11581 ccgacaacgg cgtgaagcgg atgctgtcga gccagctcga gcccgccgat gcgcgacgga
    11641 tctttccgtg ctgggacgag ccggccttca aggcgacgtt tgcgctgacg gtcacggtgc
    11701 cccgcacctt cctggcggtg agcaacatgc cggtggcgcg cgaggagccg gtcgcaccgg
    11761 acaaaaagca ggtcgcgttc gcgccgaccc cgaaaatgtc gagctacctg ttcgtgttcg
    11821 cggccggcga gctcgagcgc ctcaccgcgg agacggacgg cgtcacggtc ggcgtggtca
    11881 cgacggcggg caagcgcgag caaggccgct tcgcgctcga aaacgcggtc aagctgctgg
    11941 attactacaa cgattatttc gggatcaaat acccgctgcc taaactcgac ctgatcgcgg
    12001 tgccgggcgg tttcggcggc gccatggaga actggggcgg gatcaccttt ttcgagagcc
    12061 ggctgctgtt cgacccctcc accaatccgg atacggcacg gcgcggcatc ttcagcgtgc
    12121 tggcgcacga gatggcgcat cagtggttcg gcgatctcgt caccatgggc tggtgggaca
    12181 acctctggct caacgagggc tttgccagct ggatgcagga gaaggccgcg gaacatttct
    12241 atccgcaatg gcagacctgg ctcaacggct acggccagaa gcagtacgcc atggcgctcg
    12301 acgcgcggcg cacctcgcat ccgatccagc agccggtcgc cgaccacagc gaggccaacg
    12361 ccgcgttcga cggcattacc tacaacaagg gccaggcgct gatccgcatg ctcgaaaacc
    12421 atctcggcga aagagcgttt cgcagcggca tccgcaaata catggccgcg cacgcctatg
    12481 gcaacaccac cactgccgat ctctggcaga ccctggaaag cgctgccgga aaaccggtga
    12541 cgggcattgc cgcctccttc accgagcagg acggcgtgcc gctgatcgtg gccgaaacca
    12601 gctgcagcgg cgacatgcag cggatgacgc tgcggcagga tcgatttgtg ataattcccg
    12661 cggcttcctc cgccgcgagc acgcaagcga ccgtcttgcc gcctcgaacc tggcaagtcc
    12721 cgatctcggt cgggccgctg cgcgcaacgc agccggcgga acacctgctg cttgcgggca
    12781 gcagcgacat cgccgccggg tcctgtggcg aggcggtcaa agtcaatctc ggcgacgtcg
    12841 gctattaccg gaccgaatat ggccccaaca gccgggccgc gctggcaaag tcgctggcac
    12901 tgatgacgcc ggatgatcgt gtcaacttcc tcgccgacag ctgggcgctg gtgcaggccg
    12961 gccgcgccga accgccgtct taccttgcgc tgatcgaaca gatcagccgc gacgaccgcc
    13021 gcgcggtatg ggatcaggcg atcggcagct tgaccacgct cgatcgtctg gcgcgcgatc
    13081 gcgccgaacg cccggcgctg cagggctatg cacgcgcaag attgcgcccg ccgttcgatc
    13141 ggctcggctg ggacggcagc ggttcaggcg acgacgaaaa caccttgctg cgcgccagcc
    13201 tgatctccgc gctcggccaa ttcggcgacg aagatattct cgccgaagcc cgacgccggt
    13261 tcgcggcctt cgtgcaaaac ccgcaatcgc tgccgaacgc actgcgcgat cccgtcaccc
    13321 atctcgtcgg catcagcgcc gaccgcgcaa gctacgacac gctgctggcg ctcgcgcgca
    13381 aaagcacggt gaccaacgag cgcctgcgct actattacgc cgccgccagc gcgcgcgacg
    13441 ccacgctggc gcgtgccacg ctggcgctga ccttgaccga cgaattgccg aacacgatcg
    13501 ttggcggcct tatcaacacg gtcgcctcct ccggcgagca gcctgatctg gcctgggaat
    13561 tcgtgcagac gaatttcgat gcgctctcgg caaaacaggg accgtcgttc cgcgacgcct
    13621 tcgtcgccaa cttcatgacc aacttcaccg atgacgccca tgccgccgag ctctcgcatt
    13681 ttgcaccggc gcaggcgacc tccggtggcc gcgtgataac ggctcgcgcg ctggagacga
    13741 ttgcgatctc ggccgatctc aagacgcgcg cgttgccggc ggtgaatgcc tggatcaaag
    13801 agcacaatgg cgggcggccc tgaagcaacc gcatgacctg accggctttt tatgacaccg
    13861 gccaggctgc gacattctta caccgttaga accgtcgatc ccgtggtctt gcgcgcggcg
    13921 agatcggcgt gggccttggc cgcgtccttc agcggatagg tctggttgac ctcgatcttg
    13981 accgcgcctg atagcaccac ctcgaacagt tccttcgcca tggcgacgag gttctcgcgc
    14041 ctggcgccat aggtattgag cgtcgggcgg gtgacgtaga gcgagccctt ctgcgccagc
    14101 aggccgaggt tgagcggctc gacgctgccg gaggattggc cgaacagcgc gacgacaccg
    14161 agcggcgcca ggcagtcgag cgatttcaag aaagtgtcct tgccgacgga gtcgtagacg
    14221 acaggcactt tcttgccttc ggtgatctcg cccacccgct tcacaaaatc ctcgcgcgtg
    14281 taaaccacgg tgtgggcgca accatgcgcc ttggcgagtt ttgctttttc atcgctgccg
    14341 acggtgccga tcacggtagc gccgagatgc ttcgcccatt ggccgaggat gaggccgacg
    14401 ccacctgccg cggcatgcag caggatggta tcgccggcct tgacccgata ggtctggcgg
    14461 atcagatatt gcaccgtcag gcccttgagc atcatggcgg ccgcggtctt gtcatcgatc
    14521 ccgtcgggca gtttcaggag acgatcggcc ggaatcagcc gcgcctcggc ataggcgcca
    14581 agcggtccat tgccgtaggc gacgcggtcg cccggcttca gatcgctgac accgggtccg
    14641 acttcttcga ccacgccggc gccctcgctg cccagcccgc tcggcacctc tgtgggataa
    14701 aggcccgagc gattatagat gtcgacaaag ttcagtccga cggcggtgtg gcggatgcgc
    14761 gcctcgccgg gaccgggctt gccaggtttg acttcctccc agaccagaac ttcggggccg
    14821 ccggtttgat gaaagcggat ggcgtgggtc atggaattac tcctcacatg ggatgacaag
    14881 gaaagaaaag aaatgccgca gcggagggga tccgccagcg ggcgtctaga attgttcacg
    14941 catcgatcgc atccttgatc cgctttcgcg tcaaaggcaa atcgcgcagc cgcttgccgg
    15001 tggcgtcggc gatggcgttg gcgatggagg ccgcggccgg cccctgtcca gtttcaccgc
    15061 ttcctaggaa aggttttccg ggccggttga tgatgtgcac ctcgatgctc tcggggaccg
    15121 agttgaaccg caggatcgga taggtttgcc agtcgatgct ggtgatcctg gtgtcatcga
    15181 aggtgacgct ctcatacaag gtccagctca tcgattgcac gatcgcgccc tcgatctggt
    15241 tgatcagccc gtccggattg accacctgcc cactatcgac cgccgccacc gcgcgcacca
    15301 gccgcagacg cccggtctcc cggttcactt ccacttccgt ggcgatggca caataagccg
    15361 cgagattctt gtagcgggca aaggcaaagc caaatccgcg atcctgcggc gcttgttgcc
    15421 ccttcagcca tccaaaccct cgcgccgcct tctcgatcac ctcgcgcccg cgcggatcgt
    15481 tgagatgttt cagcctgaat tcgacgggat cggcgccagc cgcaccggcc agttcgtcca
    15541 tgaaactctc gatcgaaaac acattgtggt aggccccaag cgaccgcatc gccgaaatcc
    15601 gcagcggcat ggccgggatg aagtggtgca ccacatgcgc gttggggaat gtgtagatcg
    15661 ggatcgcgtt gcggtcgccg ccgccttcgg gtaacggcag cggttttggc gccggcacgg
    15721 caaacggctg tgacatgtgc tgcgccgcca gcatcgatcc cgcgcctccc ggccgcatcg
    15781 aatgggtgtt gctccagact tcgaaattcc agtccgctat cgcgccgttg tcgtcgagcg
    15841 acgccttcag ctttgtcgcc atcgccgggc cgaacggctc ccaaatgtgc tcctgttcgc
    15901 gcatccattg cacgcgaacc ggcgtacccg gcaaagcgcg cgcgatcagt gccgcgtcgg
    15961 cggcggcatc gtcggcgcca ttatggccgt aacatccgga gccttcgaca tggatgcagc
    16021 gaacgctcgc cggcggcacc cgcaacattt ccgcaatgcc ctggcggtcg ggatagacac
    16081 cttgggtgtg ggtccagacc gtcatcgcgc cgtcgaccca ttgtgcaacc gcacaggacg
    16141 ggccgatcga cccgtgcgcc tgatacggcc gggtataggt ggcttcgatg gttttttgcc
    16201 cgacggccga cggattgttc tgttggaaga tggtcgaatc ctgcgacggc aggctggtca
    16261 gcaggtgaag cagatcatcc tgcttcggca ggccagccgt ctccttccac gtggcggcag
    16321 ccgacagcgc cttcatggcc ttgaccgcca aaaattcctt tgcggcgacg accgcaagaa
    16381 aattgccgtc gcgaatgacc ttgacgacgc cgggcagttt ttcgatcgcc gaggtgtcgc
    16441 aatccgtcaa ttgggcgccg tagctcggcg gccgcacgat ccgggcgtgc accatgccgg
    16501 gtagccgcat gtcctggaca taggccgcac ctccggtgac cttggccgga atatcgacgc
    16561 gcggtatcgg ctgccccatc accttgaacg tcgcgggatc cttcagcttc gatttcggct
    16621 gcgcctgcac atgcagcatg tcggccgcga ccaggtcgcc gtaagcaaag cgccggccgc
    16681 cggacgaaat caccgcgcca ttttcggtgc gaaaattttc agccggcaga ccgagacggc
    16741 gggcggcttc ctccaccaga agttcgcgca cctgcgccgc cgcgttctgg atcgcagtgc
    16801 cgctgtcctg catcgaatga ctgccggacg tatagccttc gttggcggtg aggcgcgtat
    16861 ccgcggtgac gaccttcagc gattcgaagg gaacatccag ttcttcggcg gcgatctgct
    16921 ggaacgcggt cttgaaaccc tggccgagct cggtcttgcc ggtgaacgcg gtaatgctgc
    16981 cgtcagcatc gatgcgaatc caggagtcca ggaatggcgc gcccttgagg ctgccgggag
    17041 gattttgcac cggagccgcg gccggcgcgg tctggtcctg cgcgaaggcc gcggtgagcg
    17101 agaagctgac gatcagcgcg ccgccacctg ccagcacgcg acggcgatcg agaatcacag
    17161 aggcgttcat ttgggcgttc atctgggcgt tcatggcgcg ctcctctggc tcgccgaaga
    17221 cgcgtccgcc gtgtccatca gccggctggc gcggtgcacc gcgcgcagga tgcgcatatg
    17281 ggtgccgcag cggcacagat gcggctccag ctcgctacgg atctgctcat cggtcggctt
    17341 cggattcctc tgcagcaagg cctgcgcacg catcatcatg ccggcgatac aatagccgca
    17401 ttgcgccgcc tgctcttcca tgaaagcgcg ctggatcggc gcgggcgctt cgaccgtccc
    17461 gagaccctcg agcgtcgtca cctgcttgcc ctcgagcaga agcagcggcg tcacgcacga
    17521 caacaccgcc ttgccgtcga cgatcaccgt gcacgaaccg cattggccga gcccgcagcc
    17581 gaacttggcg gcattgagct tgatatcgtc gcgcaggaca taaagcagcg gcgtgtccgg
    17641 atcggcattg atctggtgat cgacaccatt gaccttcaat gtcatcatgg cttgtctcgc
    17701 tgcgttggat cggccggcgc gttgtggggc ccgggcgata tccgaagaaa cgcgatctgg
    17761 gtgcggcgtg cgtcctcaac agtcttttca acgtcggtcc aggccggttg gttgctgaac
    17821 cgcgcccgca aatagttcaa cagcgctgta atctggccgt cattcatgct gccggcaaaa
    17881 cccggcatga tcgggctgcg ctctccttcc acgggtcgaa gccccgacag cacgatgttg
    17941 gcgacgttgc gcgggtcggg gccagcgatg gcggtcgaaa gcgcaagatt gaccccgcca
    18001 tagggcaacg tcctgccggt ctcatggcat gtcgcgcagg ccgcggcgta gatcgccgca
    18061 ccggccgcgt ccccctttgg ggcctgcgcc gagggtgacc tgacttgtgc cagcacggct
    18121 tcgccctgac gtttgcgatc cggcgtcggc gcgccgaaca cgccggccat ataggttgcg
    18181 atggcccgga tatcgctcgg cggtaccgac gacagattgc tgaccacctg cgccatcggc
    18241 ccgcgcgcga cgccgtgatc gggatgccag ccgtcgcgga gataggcgaa cagcgcatcg
    18301 gcgtcccacg gcacgggcgc cggagattgc gcgttgatcg cataagcgtg ccaattgtcg
    18361 acatcgccac cggcaaactg cgcggtcgca cgttccgcgc cgagcgcatt gcgcggggtg
    18421 tggcatgaac cgcaatgcgc cagcccctcg accagatagg cgccgcgatt ccattccgcg
    18481 ctctgcgcca agtccggctg aaacgtgccg tgacgaagga acagcagctt ccagccggcg
    18541 atcacaaacc gctgatcgag cggaaacgaa agctggttcg cgcgggccgc cgcatgaacc
    18601 ggctgccgtg tcatcaggta cgcgtagaga gcctggtcat cttcgccggt tacattggtg
    18661 aagtgatcgt agggaaatgt cggatacaag tgctgcccgt cgcgatcgac gcctgactgc
    18721 atggcacgac gaaaggcggc ttccgaccac tggccgatcc ccgtctcggc atccggtgtg
    18781 atattggagg aaaagatcgt gccgaacggc gtcggcaccg gaagcccacc ggcaaagttc
    18841 ttgccgccgc gcacggtatg gcaatcgttg caattgccaa tcgccaccag atcgcggcct
    18901 cgcttgacca gggctgcatc gaacgactgc ggcgcgggcg gatcgatcgc agcgatcgcc
    18961 ggccgccacg cgatcgcaaa ggcagcaacc gcgcccgcca tcaagagcgc cgcgataacg
    19021 ctgccgataa ttcgtgattt tctcatccgt ccccacggct tttgcagcca ttgttaacac
    19081 aaatccgcgc ccggagtttt ccgcgagatc ccagatcacc gtcagttgac ttccggtttg
    19141 ttgagggttc cttttgttag ctcaagtcgg cggcacggcc agcgaagcca gtctggaaac
    19201 atcggtgctt ttgtcgagcg cccagatcgc atcgaccagc ggcgtcacgt cgtagcgcgg
    19261 atcccagccg gcggcggcgg agcgcaactt cgcctcgaga tcgccatcgc tcatcggatt
    19321 gacgtcgctg ccgcgcgccg ccggctgcga cagcccgtgg gtgttgccat cggcggtgaa
    19381 gatttcgacc gccgccgcga tggtggagaa cgcggaatcc cgcagcactt cgaccttgct
    19441 gcgcagcgcc agcactttcg gatcgcgcgc gcaggcggtg aactggtcga gcccggccta
    19501 gccggtcacc agggcggcgg cgaccgcgtg gtgcacgctg acctgcgact cccgcccggt
    19561 cgaaatatca ggccggtcgg tgcggtcgct gagcagcggg ttgccgcgca ccaccacgcg
    19621 cgtgacgtcg gccgtttggc ctgatgccgc accggcgctg gtatgtcgtc ccagtgcgtt
    19681 gcagcaataa agcgggcaag gctctacgtc acacccggct tgtgcaacat cactgtcctc
    19741 cgcaaaatcg gcgcaagcac gaaggcgtag agcacgaccg gcgccgtcgt gatcaccagc
    19801 acacatgtgt cccttccgac acagttacca ttatttcaaa ttgaacaaat agtattgcga
    19861 tatgcgcccg tctcaaggct tgttatggtg atcacctgac ctccagttga tacacggctg
    19921 attcttgggc ggttgaaatc ggtgtgtgct ggggagagag cgcgttccag gctcgagggc
    19981 tttgggcggg acgtgagaca aaaaaagaca aagcctggga cgctgtgagg accaaaaaaa
    20041 tgttgcatag gtctgtaacg accgcacacg tgtgcggcgt agggattttt ttgtcatctc
    20101 tgttgagcgg tggagcgtat gcggccgatc ttttaacgaa agctccgcct gttccttatg
    20161 ctgccaccga tgatttctgg acaagaccgt acctgcttgg cgatctcggc aggacgaggc
    20221 tgaaggagat gggcatcgat ctgtccctgc agctgggtga cgaagccgtc accaacgtga
    20281 cgggcggaac caataaagtg gcgacccagg ccggacagct gacctttcag ggcaagttcg
    20341 atatggcgaa gcttgccgga atcccgggcg gcttgatcgg catcacgctg gtcgaccgct
    20401 tcggccacaa tctgaatacc gaggcaggca ttcctgcctt gcagctgacc aatgaagttt
    20461 tcggccgcgg caatattctg cgcctgaccg aactctacta ctcgcagaag cttttcgatg
    20521 atcgccttga actcaagggc ggccgtcttc cagtcggctc cgatttcttc ttcggtcagt
    20581 gcgagttcat caacctgacc ttctgcggcg gccagcccgg caacatccag ggtggctaca
    20641 tctttaactt cccggtgagc cagtgggccg gtctcgccca cttcaacatc accaaggaag
    20701 tccagatttc ggccggcgtc tacgacgcca atccgaatta tctgacgacg tcagacatgg
    20761 gcgtctacct ggcgccgggc atccct
//
LOCUS       DATNOI010000398         5536 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1180953,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000398 DATNOI010000000
VERSION     DATNOI010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180953"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(421..552)
                     /locus_tag="VN968_15960"
     CDS             complement(421..552)
                     /locus_tag="VN968_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30765.1"
                     /translation="MFHHAINAVMSPVTGLASWHVDLGETMIRHALPSRTVTASQKR"
     gene            complement(764..982)
                     /locus_tag="VN968_15965"
     CDS             complement(764..982)
                     /locus_tag="VN968_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30766.1"
                     /translation="MKTPSTRLLLTIPFGIPAYWTPEQALAVVELLDELRELIWSHYD
                     MQLLDLIHDDHKPCCSDQSGSPPDDPQF"
     gene            complement(1050..1850)
                     /gene="istB"
                     /locus_tag="VN968_15970"
     CDS             complement(1050..1850)
                     /gene="istB"
                     /locus_tag="VN968_15970"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HXL30767.1"
                     /translation="MRDQLQTLLAQLRFHGMAAALDDEIERAEREATPAAELLRRLLN
                     AEAASRRQRSLAYRLDQAHLPWRWTLDTFPFERQPGINKGQINTLAGLDFVRRADNVL
                     LIGPPGTGKTGIALGLLREACLNGYRGRFYNAQALLDELYASLADRSTARLLKRLSRH
                     QPMVIDEIGYLTLKPEQANAFFRLMDERYNRVSTIITTNLDLPEWYELFQKKPLVDAL
                     LDRLQHHCITIRIDGPSLRSPDPPPATAASGRAPRKAPATKQPAAASP"
     gene            complement(1843..3231)
                     /locus_tag="VN968_15975"
     CDS             complement(1843..3231)
                     /locus_tag="VN968_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30768.1"
                     /translation="MIPMEIRNSVRVLKAQGQTLREISRLLKLSRNAVRRILREREVA
                     AVLPCPAPTLAKLDDVFARAGGNVARVQQLLAAETDLQVPYSTLTRWIREAGLRSPPR
                     RAGEYSFAPGEEMQHDTSPHRMMMAGKAITAQCAGLVLAFSRRLFIQYYPRFTRFEAK
                     HFLLEAARFMDGTCPLCIIDNSSVIVAAGAGADAVIAPEMHAFARTLGFGFRAHRVGH
                     PDRKGRIERPFAYVEGNFLPARSFDDFADLNRQALAWCRDVANQKQKRVLGMSPEAAY
                     LIEKPHLQTLPGALPPVYDVLERVVDLHGYVSIDTNRYSVPERFVGLSVTVYKYPADI
                     RICRRGSEIATHPRLIGQRDARHTLPEHHPTPARASRTPALEEKLLRGDHESLARYAA
                     ALKQRTHGGGLRALRRLLEMKRTYPAGPFLAAVEQALHFGMFDLARLETLVLRQVAGD
                     FFDLDTAGDDDA"
     gene            complement(3137..4132)
                     /locus_tag="VN968_15980"
     CDS             complement(3137..4132)
                     /locus_tag="VN968_15980"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HXL30769.1"
                     /translation="MDTPWLDLDLHRLELRFAGARLVEPRAVERIARSIEQGGQIVPC
                     IAVADPPVKAQEGGERLVLIDGYRRVAALRRLGRDTARVECWPCDLTEALLGVLARAQ
                     GRPFAAIEEALQVREITQGLGLSQHEVARRCGRDVSWVSRRLQLLSALPDAAVVAVRE
                     GRLSSWAAARVMAPLARANGEHADRLLRALAGAPLSTRELRCWFKHYEGASRVTRERL
                     VDQPQLFVRAQQESAAQHTSERLRDGPEGECETDLRHLNVIVARLRKRMPTLRPLPEA
                     VVNAVPRLQTSFDALCNDIKRYAEHDPDGDTQQRAGSQGARSDLARDQPPAQAVA"
     gene            complement(4806..5219)
                     /locus_tag="VN968_15985"
     CDS             complement(4806..5219)
                     /locus_tag="VN968_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30770.1"
                     /translation="MKFTPLVTIEKKFRKGYPQDNIIFEDSHQAVLIQNKQRVMRCGI
                     DEPVQLQTLLELFFRYERAEIADFRKAVEQFQADLPEVLKALREMIETAERNNEKFRK
                     ASLRFLKHAQDTINPSASTLTGMDPGVLIGMDPPC"
BASE COUNT          986 a         1725 c         1864 g          961 t
ORIGIN      
        1 atcctgccat ggtgcttctc tttcacgcga ccagccaatg ccgctcggca aacatgcgaa
       61 gcctcggcgt gcgccggttg gaattctgtc agcgccggcc gctcgcaatg acgctcaaat
      121 agccgaaaat agccagaacg aagaggcacg ccagaaacac gcctcgacgc tcacgggaat
      181 ggacccacct gtgctcactt tttcggacct accaagtgcg cagacgcgag tgacggcgca
      241 ggttgagcca cggagtgcaa ggggggcccg cgtatcgcgg agcgaccgcc acaagttacg
      301 cgcagggatg cgtgcgtagg gggacctgtg gaccggcgtg ggtgaaccgg gagggcgggc
      361 tctgttggtg gcggtgaatg cgcaccgata gatggcgtcg aacgctgctg cgattagatt
      421 ctatcgcttt tgggacgccg ttaccgtgcg cgacggcaac gcgtgacgga tcatggtttc
      481 gccaagatcg acgtgccacg aggcaagccc ggtgaccggc gacattacgg cattgatcgc
      541 atggtggaac atgctgctcc ccgccgtcgg tctgtcaagc ccgaagccgc caacggcggt
      601 ggcccgaagg gccaggcttt acagaccgat ggcggggagc agacaattga acttgcgatc
      661 ggacaacgcg cacaacgtcg gcagcggcgc ctccggcgct tcgccatggg gcaagctctt
      721 cgcgatttat gcgcgcatcg tcaaaaccgc tgacatgagt ttctcagaac tgcggatcgt
      781 ctgggggcga tccggattga tcggaacagc agggtttgtg atcgtcgtgg atcaggtcga
      841 gcaactgcat atcgtagtgg ctccagatca gctcccggag ttcatcgagc agctcgacga
      901 cggcgagggc ctgctccggc gtccagtaag cgggaatgcc gaaggggatg gtcaggagca
      961 agcgggtgga gggcgttttc atgacgggac tccgcggtgc atgggtgcgg ctcggtcatg
     1021 gcgacggggc cgtggcgcct ataaaggtgt catggcgatg ctgcggcggg ctgcttggtt
     1081 gcgggggctt tgcgcggcgc gcggcctgat gcggctgtgg cgggcggcgg atccggactt
     1141 cgcaacgacg ggccgtcgat gcggatggtg atgcagtgat gctgcaggcg gtcgagcagg
     1201 gcatcgacca ggggcttctt ctggaacagc tcgtaccact cgggcaggtc gagattggtg
     1261 gtgatgatgg tggagacgcg gttgtagcgc tcgtccatga ggcggaagaa ggcgttggct
     1321 tgttccggct tgagcgtgag atagccgatc tcatcgatga ccattggctg atgtcggctc
     1381 aagcgtttga gcagccgcgc ggtggagcgg tcggcgagcg aggcatagag ttcgtcgagc
     1441 agtgcctggg cattgtagaa gcggccgcgg tagccgttga ggcaggcttc gcgcaggagc
     1501 ccgagggcaa ttccggtctt gccggtgccg ggcggcccga tgagcaggac attgtcggca
     1561 cgacgtacga agtcgaggcc ggcaagggtg ttgatctgcc ccttgttgat tcccggctgg
     1621 cgttcgaacg ggaaggtatc gagcgtccaa cgccacggca gatgtgcctg atcgaggcgg
     1681 taggcgaggc tgcgttggcg tcgcgacgcc gcctctgcgt tgagcaggcg acggagcagt
     1741 tcggcggcgg gcgtggcctc acgctcggca cgttcgatct cgtcatcgag ggcggcggcc
     1801 atgccgtgga agcgcaactg ggcaagcagg gtttgcagtt gatcacgcat cgtcgtctcc
     1861 cgcggtatcg agatcgaaga agtcgccggc gacctgtctg aggacgagcg tctccaggcg
     1921 ggcgagatcg aacatgccga agtgcagcgc ctgctcgacg gcggccagga agggaccggc
     1981 cggataggtg cgtttcatct cgagcaggcg acgcagggca cgcaggccgc cgccatgggt
     2041 gcgctgcttg agggcggcgg catagcgtgc gaggctttcg tgatcgccac gcaacagctt
     2101 ctcctcgagt gcgggcgtgc ggctcgcccg tgccggcgtg ggatggtgct ccggcagggt
     2161 gtggcgggcg tcgcgctggc cgatcagccg gggatgtgtg gcgatctcgc tcccgcgccg
     2221 gcagatccgg atgtcggccg gatatttgta gacggtgacc gacagcccga cgaagcgctc
     2281 cgggacggag tagcggttgg tatcgatcga gacgtagccg tggagatcga cgacgcgctc
     2341 cagcacgtcg tagaccggcg gcagcgcgcc gggcagcgtc tgcaaatgcg gcttctcgat
     2401 cagataggcc gcctcggggg acatgcccag cacgcgcttt tgcttttgat tggcgacatc
     2461 acggcaccag gccagcgcct ggcggttcaa atcggcgaag tcgtcaaagc tgcgcgccgg
     2521 caggaagttg ccttctacat atgcaaaggg tctttcgatc cggcctttgc gatcaggatg
     2581 gccgacccgg tgagcgcgga agccaaagcc cagcgtgcgg gcaaaggcat gcatctcggg
     2641 cgcgatgacg gcgtccgcac cggcgccggc ggcgacgatg acgctggagt tgtcgatgat
     2701 gcagagcggg caggtgccgt ccatgaagcg cgccgcctcg agcaggaagt gcttggcctc
     2761 gaagcgggtg aagcgcggat agtattgaat gaacaaccgg cgcgagaaag ccagcaccag
     2821 cccagcgcac tgcgcggtga tcgccttgcc tgccatcatc atacgatgcg gcgaggtgtc
     2881 gtgctgcatc tcctcgccgg gtgcgaagga atactcgccc gcgcgccgtg gcgggctgcg
     2941 caggcccgcc tctctgatcc agcgcgtcaa ggtgctgtag ggcacctgca gatcggtttc
     3001 ggcggcgagc agctgctgca cgcgcgctac gttgccgccc gcacgcgcaa agacatcatc
     3061 aagcttggct agcgtcggcg ccggacatgg cagcaccgcc gcgacctcgc gctcgcgcag
     3121 gatgcggcgc accgcattac gcgacagctt gagcaggcgg ctgatctcgc gcaaggtctg
     3181 accttgcgcc ttgagaaccc gcacgctgtt gcgtatctcc atcgggatca tgctcggcat
     3241 accgtttgat gtcgttgcaa agggcgtcga agcttgtctg caggcgagga acggcgttca
     3301 ccacggcctc gggcaacggg cgtagcgtcg gcattcgctt gcgcaggcga gcgacgatga
     3361 cgttgaggtg ccgcagatca gtctcgcatt cgccttccgg gccgtcgcgc aagcgctcgc
     3421 tggtgtgctg cgcggcgctt tcctgctggg cccggacaaa cagctgcggt tggtcgacca
     3481 aacgttcgcg ggtgacgcga ctggccccct cgtaatgctt gaaccagcag cgcagctcgc
     3541 gggtggacag cggcgcgccg gccagcgccc gcaacagtcg atcggcatgc tcgccgttgg
     3601 cgcgcgccaa cggcgccatc acccgggcgg ccgcccagct cgacagccgg ccctcgcgca
     3661 ccgccaccac agccgcgtcg ggcagcgccg agagcagctg caggcgccgg ctgacccagc
     3721 tgacatcgcg cccacagcgg cgggcgacct catgctgcga cagccccagg ccctgcgtga
     3781 tctcgcgcac ctgcaaggcc tcttcgatcg ccgcgaaggg ccggccttgg gcgcgcgcca
     3841 gcacgccgag cagggcctcg gtcaaatcac agggccagca ctcgacccgc gcggtgtcgc
     3901 ggccgagccg gcgcagcgcg gcaacgcgcc ggtagccgtc aatcagcacc agacgctcgc
     3961 cgccctcctg ggctttgacc ggtggatccg ccaccgcaat gcacgggacg atctggccgc
     4021 cttgctcgat cgaacgggcg atccgctcca ccgcacgcgg ctccaccagg cgggcgccgg
     4081 caaagcgcag ctccaggcga tgcagatcaa gatccagcca cggcgtgtcc atgccggcaa
     4141 gcctagcagc gtggccgccg cgcccctatc acgtcctctc tttgcaaact tccccggctt
     4201 gcccgtatgg cgttgatacc aaagtgattt ccaccatcca cgcatccatc tttgcaaact
     4261 ccgcaggggc tgccgggacg agccgataaa cccctgaagg cacgggtgaa tccggcatcg
     4321 acgcgtccat ctttgcaaac cctgcagagc ccggcgaagt cgcatcccgc cggcgattac
     4381 gctgccgctg ccgggcagcg gcgcgattgc tctcgctgta ttgcgggtgc gtgcgccgat
     4441 aatcgcgcca gtattcgcga tggctttgcg cccacgcacg ctgtgcccgt gcctggttct
     4501 cgcgatagtc cgcgtcgctg gcgcgctttg cttgctgcca acggcgccgt cgttcacgct
     4561 gacaggctga agtgccgcaa taacgctgct gtggaacctg cgaacgcggc cgaaatgcct
     4621 gcccgcaccc cgcacattgc cgcctctcca caacgcccct ccgcgcagat aacctgcaca
     4681 gagcgtccac cgccggcccc taatacaatc tcaccccgcc gcacatgcaa tcgcgcccct
     4741 cggccccaga caaggcgctt ccgcagtcgc atttcggtca ggtcacaggc cggctaccgc
     4801 ccacctcagc aaggtgggtc cattccaatg agcacacccg ggtccattcc cgtgagcgtc
     4861 gaggcgctcg ggttgatggt gtcctgggcg tgcttgagga agcgcaacga agctttgcgg
     4921 aatttttcgt tgttgcgctc ggcggtctca atcatctcgc gtagcgcttt gagcacttcc
     4981 ggcaaatcgg cctggaactg ctcgacagcc ttgcggaagt ccgcgatctc tgcgcgctcg
     5041 taacggaaaa acagctctag caacgtttgt agttgcacag gctcgtcgat accacaacgc
     5101 atcacccgct gcttattctg gatcagcact gcttggtgtg aatcctcgaa gattatgttg
     5161 tcctgcggat agcccttgcg gaactttttc tcgattgtca ccagcggcgt gaatttcacc
     5221 taaagccacc ggtttgaatt tccctatgat gtggcaagcg cctccagccc cgatccgatc
     5281 aaggatgccg ccgtgctcgg gccaatcaag gccgagccct tcgggtggcc gcgaagacgc
     5341 ggccagcctt gacaggccct gcgcgcgacg gctgcgagat tcggcggtcg ggatggaaga
     5401 atgcgcgcgg cgcgggtcga accaaagaat ggccccaaag agaacaagaa aattggcaat
     5461 ttcaaaccgc cccatttgcg ggggtacaac gcagtggtta ctctacaaag cgacgggaca
     5521 agaacctata ggggaa
//
LOCUS       DATNOI010000399         7750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_538858,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000399 DATNOI010000000
VERSION     DATNOI010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538858"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VN968_15990"
     CDS             <1..1176
                     /locus_tag="VN968_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HXL30771.1"
                     /translation="LADLDRQLREEADKVSRSLESDARIASARVDGLTNSLDQLKKQA
                     TSSNGQDVQLRALEREAKAQRDLLESYLAKYREATTRENIDAAPADGRIISSASVSNT
                     PAYPKKLPIVLIATLATLMLSTGAIATGELLRMTAPRTTAVLTSPPDPVERAPAPAPA
                     PAAAAAAVAAADPAPVPKEAHPDIATGLTEIEQLADDVNTAGDAARKVTILGTAANES
                     ITLTALTLARLVARNSRVVVVGLSASSSEISTISAASVDPDAPGLAELMLGETSFSQV
                     ITKDRSSRVHLVSAGRPGSDRALLQSPRLALAIDALLRVYDHVLLDAGTASDLPAELL
                     TAQARAVVVPDPSMTADARTSMCDQLRAAGCFTELTLLSKSLQPSDAMEAGARVVAA"
     gene            complement(1214..2407)
                     /locus_tag="VN968_15995"
     CDS             complement(1214..2407)
                     /locus_tag="VN968_15995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL30772.1"
                     /translation="MTMAAAIESRTADELAWSKSSRIAGVDIFHDLVQAEAIWRALED
                     PRQFSTPYQRFDFLGHWQHQVGEREGLSPFIVIACDAERRPLLLLPLTLRHQCGVRTA
                     CFMGGKHATFNMALWDRDFAANATAVDLEALISGIRARSAADVLALTRQPLHWRDLPN
                     PMALLPSQASANDCPLMTIAPDEPPTARISNSFRRRLKGKERKLQSLPGYRYGVAVTD
                     AEIKRLLDWFFLVKPLRMAEQKLPNVFAEPGVEEFVRGACMAPVPGGGHVIDIHALEC
                     DDEVIAIFAGVADGHRFSMMFNTYTMSDNSRVSPGLILMRNIIDHHAERGYRALDLGI
                     GSDDYKRLFCKGDEPIFDCFIPLSLRGKMAAAAMSGINRAKHLVKHSPALLHMAQKLR
                     GALAR"
     gene            2538..3590
                     /locus_tag="VN968_16000"
     CDS             2538..3590
                     /locus_tag="VN968_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXL30773.1"
                     /translation="MASGSPFLARAGLELAYLSGYSRLMQRRTGGAGVILRFERVRPR
                     RTARFQPLQACEITPRFLDRAIGALKRWKFDIVSMDEVCRRAVTLASPRRFVCLTFDG
                     GYKDMITSAYPVLAKHGVPFTTYVPTAFPDRLGEAWWLALEEVIAREDRISLVMDRRE
                     QRFNVGDTSEKYQLYDFLAGWMRALSPPDLSVAINDLCKRYSVNLAALSREAMMDWDD
                     LTKLAADPLVTMASATVNYPVLSNLRDADALREMTMGRAVAEAAFRRDVRHFAYPFGD
                     RAAFGRQHVGMAEEAGFASAVSAISGVVQSEGRTNLHALPRIAWDGRRRSLRVMRVIL
                     SGVTFRPAKRGPDTKF"
     gene            complement(3715..3921)
                     /locus_tag="VN968_16005"
     CDS             complement(3715..3921)
                     /locus_tag="VN968_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HXL30774.1"
                     /translation="MTIRTRKFFGTIALLVLVVVWSLMGMTVAQTPWLANSGLLQAIF
                     YVVAGIGWVLPAMPIVSWMARPDV"
     gene            4017..5108
                     /locus_tag="VN968_16010"
     CDS             4017..5108
                     /locus_tag="VN968_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HXL30775.1"
                     /translation="MTGISAQQASPTRAVRWWLFGVAALIAIMVLVGGATRLTESGLS
                     IVDWKPVTGTLPPLSQDQWTQAFDAYKTIPQYRELNASMSLAEFKTIFWWEWSHRLLG
                     RVIGIAYLLPFLWFLWRGVLSTDVRRRLWLIFGLGALQGAVGWWMVASGLSQRVEVSQ
                     YRLATHLVLALLIFAGIVWTLRRLASRPSLVASSRLKITGVALVVLTFVQLYLGALVA
                     GLRAGKVYNTWPDIDGALIPSAARLFFEEPWWRNLFDNSLTVQFEHRMTAYALFALAV
                     LHALDAVRSRAAPAVVNGAFWLAAAITLQATLGILTLLNQVPTSLALAHQAVAIAVLT
                     LAIFQAERLAARQPTPAQQNLAVPVGQPG"
     gene            complement(5142..5495)
                     /locus_tag="VN968_16015"
     CDS             complement(5142..5495)
                     /locus_tag="VN968_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL30776.1"
                     /translation="MSGSIDLSEKLATFSEHFSPRTVAQFNNCDVMVVKLKGEFVWHK
                     HDDTDDFFLVLKGVLDIQLRDRTVTLGPGQMYIVPKGFEHRPVANEEVHLLLIEPAGT
                     PNTGDVKTAAPRKTA"
     gene            complement(5763..7043)
                     /locus_tag="VN968_16020"
     CDS             complement(5763..7043)
                     /locus_tag="VN968_16020"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HXL30777.1"
                     /translation="MTDRLPGFSTLAVHAGAQPDPTTGARATPIYQTTSFVFNDADHA
                     ASLFGLQAFGNIYTRIGNPTQAVLEERLAALEGGTAALAVASGHAAQVVVLQQLLMPG
                     DEIIAARKLYGGSINQFTHAFKSFGWNVVWADPDEIGSFERAVTPHTKAIFIESIANP
                     SGSVTDIEAISAVARKAGVPLIVDNTLATPYLIRPIDHGADIVVHSLTKFLGGHGNSI
                     GGIIVDAGTFDWSKDNKYPMLSEPRPEYHGIKIQETFGNFAFAIACRVLGLRDLGPAI
                     SPFNAFLILTGIETLPLRMQKHCDNAKAVAEWLLAHPAVAAVNYAGLSGDKYNNLARK
                     YAPKGAGAVFTFSLKGGYEAGVNLVSKLKLFSHLANVGDTRSLVIHPASTTHSQLDDA
                     AKIRSGAAPDVVRLSIGIEDKEDLIADLEQALSA"
     gene            complement(7271..>7750)
                     /locus_tag="VN968_16025"
     CDS             complement(7271..>7750)
                     /locus_tag="VN968_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXL30778.1"
                     /translation="FAFKYLAQRGYDMIPVNPGHVGKSLLGKPFVASLRDIGHPVDMV
                     DIFRNSSHIMPVVDEALELVPPPRIIWMQLGARDDAAAAKAEAAGLKVVMNRCPKIEY
                     GRLSSEISWMGVNSRTLSSKRAPMPTQGMRLSLNRMSVGGGETTASDRAAKNKNDLA"
BASE COUNT         1432 a         2350 c         2466 g         1502 t
ORIGIN      
        1 ctcgccgatc tcgatcggca attgcgcgag gaggcggaca aggtgtcccg ctcgctcgag
       61 agtgatgccc gcatcgccag cgcccgggtc gatggcttga cgaacagcct cgaccagttg
      121 aagaagcagg cgacttcgag caatggccag gatgtgcagc tgcgggcgct ggagcgggaa
      181 gccaaggcgc agcgcgacct cctggaatcc tatctggcga agtatcgcga ggcgaccacc
      241 cgcgagaata tcgacgccgc gccggcggac ggcaggatca tctccagcgc cagcgtttcc
      301 aacaccccgg cctaccccaa gaagctgccg atcgtgctga tcgcgacgct ggcgacattg
      361 atgctcagca caggcgccat cgcaaccgga gaacttttgc gcatgacggc gccccgcacc
      421 acagcggtgt tgacgtcccc gccggatcca gtggagcgcg cgccggcgcc ggcgccggcg
      481 ccggcggcgg ctgctgccgc tgtcgccgcc gccgatcccg ctccggtgcc caaagaggcc
      541 caccccgaca tcgcgaccgg gcttacggag atcgagcagc tggccgacga tgtgaacaca
      601 gcgggcgacg ccgcacgcaa agtcacgata cttggcacgg ccgcaaacga aagcatcacc
      661 ctgacggcgc tgacgctggc gcggctggtc gcgcgcaatt caagggtcgt ggtggtcggt
      721 ctgtcggcgt cgtcgtcgga gatctcaacg atctcggcgg cctcggtcga tccggacgca
      781 ccgggtcttg cagaactgat gctgggcgag acctccttct cgcaagtgat caccaaggac
      841 cgctcgtcgc gggttcatct ggtgagcgcc ggacgtccgg gctcggaccg cgcgttactg
      901 caatcgccgc gactggcgct tgcgatcgat gcattgctgc gggtctatga tcacgtgttg
      961 ctggatgctg gcactgcctc cgacctgcct gcggaactgc tgacagcgca ggcgcgcgcc
     1021 gtggtggtgc cggatccgtc gatgaccgcg gacgcccgga cctcgatgtg cgatcaactg
     1081 agagcagccg gttgtttcac cgaattgacg ctgctgagca aatcgctgca gccctcggat
     1141 gccatggagg caggcgcgcg ggtcgtggcg gcctgaacgt gatcatcgct tcgtttcgag
     1201 agaaacgccg cagtcagcgg gcaagcgcgc cgcgcagctt ttgcgccatg tgcaacagcg
     1261 ccggactatg cttcaccaga tgcttggcgc ggttgatgcc tgacatcgcg gccgcagcca
     1321 ttttaccgcg caggctgagc gggatgaagc agtcgaaaat cggctcgtcg cccttgcaga
     1381 acagccgctt gtaatcatcc gatccgatcc cgagatcgag cgcacgatag ccgcgctcgg
     1441 catggtgatc gatgatgttg cgcatcagga tcagtccggg gctgacccgc gaattatccg
     1501 acatggtgta ggtattgaac atcatcgaga accgatgtcc atcggcgacg ccggcgaaga
     1561 tcgcgatcac ctcgtcgtcg cattcaagcg cgtgaatatc gatgacgtgg ccgccgcccg
     1621 gaaccggcgc catgcaggca ccgcggacaa attcctcgac gccgggctcg gcgaacacgt
     1681 tgggcaattt ctgctccgcc atccgcagcg gcttgaccag aaagaaccaa tcgagcagcc
     1741 gcttgatctc ggcatcggtg acggccacgc catagcgata gcccggcagc gattgcagct
     1801 tgcgttcctt gcccttgagc cggcggcgga acgaattgct gatccgcgcc gtcggcggct
     1861 catcgggagc gattgtcatc agcggacaat cgttggccga ggcctggctc ggcagcagcg
     1921 ccatcgggtt gggcaaatcc cgccagtgca acggctgccg ggtcagcgcc agcacgtcgg
     1981 ccgctgatcg cgcgcgtatt cccgatatca gcgcttcgag atcgacagcg gtcgcgttgg
     2041 cggcaaaatc cctgtcccag agcgccatgt tgaaggtggc gtgcttgcca cccatgaaac
     2101 aagcggtgcg gacgccgcac tgatgcctga gcgtgagcgg aagcagcaac aacggacgac
     2161 gctcggcgtc gcaggcgata acgatgaacg gtgaaagacc ctcgcgctct ccgacctggt
     2221 gttgccagtg cccaagaaaa tcgaaccgct ggtacggcgt ggagaactgc cgcggatctt
     2281 cgagcgcgcg ccagatcgcc tcggcctgga caagatcgtg aaaaatatcg acgccggcaa
     2341 tacggcttga tttcgaccac gcgagctcat ccgccgttcg gctttcgatc gcggcagcca
     2401 tggtcattgc aaaacctgtc tacgggaaat tatttatatt tttcggcgat tgcgaccttt
     2461 gcaaagaaag gtcaacaaag agtaatgaca gggcggtcgc agcggggtgg aaatatccgc
     2521 atggagggga cgagacggtg gcatctggga gcccttttct ggcacgtgcg gggctggaac
     2581 tcgcttatct cagcggctac tcacggctga tgcagcggcg caccggcggt gccggtgtca
     2641 ttttgcggtt cgaacgcgtg cgtccgcggc gcaccgcgcg ctttcaaccg ctccaagctt
     2701 gcgaaatcac gccgagattt ctcgatcgag cgatcggtgc gttgaagcgc tggaaattcg
     2761 atatcgtttc aatggacgag gtttgccggc gggcggtgac gctggcctcg ccgcggcggt
     2821 ttgtctgcct gacattcgac ggcggctaca aggacatgat cacgtcagcc tacccggtgc
     2881 tggcgaaaca cggcgttccc ttcaccactt atgtgccgac ggcgtttccg gaccgcctgg
     2941 gcgaggcatg gtggctggcg cttgaagaag tgattgcgcg cgaggaccgc atcagcctcg
     3001 tgatggatcg cagggagcag aggttcaatg tcggcgatac gtcagaaaaa tatcagctct
     3061 acgatttcct cgccggctgg atgcgcgcgc tgtcgccgcc agatctgtcg gtcgcgatca
     3121 atgacctctg caagcgatac tccgtcaacc tcgcggccct gtcgcgcgaa gcaatgatgg
     3181 attgggatga tttgacaaaa ctcgcggccg atccgctggt gacaatggcg agcgcgacag
     3241 tgaattatcc tgtgctgtcc aacctcaggg atgccgacgc gttgcgcgag atgacgatgg
     3301 ggcgggcggt cgcggaagcc gcattccggc gggatgtccg gcatttcgcc tatccgttcg
     3361 gcgatcgcgc tgcgtttggc cggcaacatg tcgggatggc cgaggaggcc ggcttcgcca
     3421 gcgcggtgtc ggcgatatcg ggcgtcgtcc agagcgaagg ccgaaccaac ctgcacgcgc
     3481 ttccgcgcat cgcctgggac ggacgacggc gctcgctccg cgtcatgcgt gtgattcttt
     3541 ccggcgtcac attccgtccg gcgaaacgcg gtccggatac gaaattctag cgcatgatcc
     3601 ggaaaagcat gccctcgggc cacgacccga tgggtgggta ccggttttcc gaaaagatca
     3661 tgctcaaaca aaaagataga gcgggatgac gattcgaaga aaagtcatcc tgctctagac
     3721 gtccggccgc gccatccagc tgacgatcgg catcgccggc agcacccagc caatgccggc
     3781 cacgacatag aagatggcct gcagcagccc ggaattcgcc agccacggcg tctgcgccac
     3841 cgtcattccc atcagcgacc agaccaccac gagcacgagc agggcgatgg ttccgaagaa
     3901 tttgcgggtg cgtatggtca tggcgaaaat gtgtggcccg ttgcaggtcg gcgcgccttg
     3961 cgcgcgggaa ggggcggact ataaggggcg cgcaaattca ttcaagcgca gcttcgatga
     4021 ccggtatttc cgcacaacag gcatcaccga cccgcgccgt cagatggtgg ctgttcgggg
     4081 tcgccgcgct gatcgcgatc atggtgctgg tcggcggcgc cacgcgcctg accgaatccg
     4141 ggctctcgat tgtcgactgg aaaccggtca ccggaactct gccgccgctc agtcaggacc
     4201 aatggacgca ggcgttcgac gcctacaaga ccattccgca atatcgcgaa ctcaacgcca
     4261 gcatgagtct ggcagagttc aagaccatct tctggtggga atggagccac cggttgctcg
     4321 ggcgcgtgat cggcatcgcc tatttgctgc cgtttctctg gttcctctgg cgcggtgtgt
     4381 tgagcacgga cgtaaggcgg cggctgtggc tgatcttcgg ccttggcgcg ttgcagggcg
     4441 cggtcggctg gtggatggtg gcctccgggc tttcgcagcg ggtcgaggtt tcgcaatatc
     4501 gcctcgccac ccatctggtg ctggcgctgc tgattttcgc cggcatcgtc tggacgctgc
     4561 ggcgactggc gagccggccg tcattggtag catcttcgcg gctcaagatc accggcgtgg
     4621 ctctcgtggt gctgaccttc gtgcagcttt atctgggcgc actggttgcg ggcctgcgcg
     4681 cgggcaaggt ctacaacacc tggccggata tcgatggcgc gcttattcca tccgcggcgc
     4741 ggctgttctt tgaagagccg tggtggcgca atctgttcga caattcgctc acggtgcagt
     4801 tcgagcatcg catgaccgcc tatgcgctgt ttgcgctggc ggtactgcac gcgctcgacg
     4861 cggttcgttc gcgggcggcg cccgccgtgg tcaacggcgc attctggctg gcggccgcga
     4921 tcacgttgca ggcgacgctc ggcattctca cgctgttgaa ccaggttccg acgtcgctcg
     4981 cgttggcgca ccaggccgtc gcgattgcgg tgctcacgct ggcgattttt caagccgagc
     5041 gcttggccgc acggcagccg acgccggcgc agcaaaacct cgcggtgccg gtcggccagc
     5101 ccggctgaac cagcgttttg caaaatcgcc tccaaaccgg atcacgccgt cttgcgcggc
     5161 gccgccgtct tgacatcacc ggtgttgggt gtcccggctg gctcaatcaa cagcaaatgc
     5221 acctcttcgt tcgcgacggg acgatgctcg aagcctttcg gtacaatgta catctgcccc
     5281 ggcccaaggg tgaccgttcg atctcgaagt tgaatgtcga gcacgccctt caggacgaga
     5341 aaaaagtcat cggtgtcgtc atgtttgtgc caaacaaatt cgcctttcaa tttcacgacc
     5401 atgacatcgc aattgttgaa ctgggcgact gtacgcggcg aaaagtgctc ggaaaacgtc
     5461 gccagcttct ccgacaaatc aatgctaccg ctcatgggtc cctccgacat cgtcatttga
     5521 gagtttagca tcaggcatgc tgtctttaaa atcgtatggt tcttggtctg cccggaggat
     5581 tacgctgggc gcgcgatgct cggtgatttg cccgccttgc aaacctcggg tgtgtcaggc
     5641 cgcgagatcg cgaagttgtg tgtatcgctg ttttgaaaat cgaatccgta gcccggatga
     5701 gcaaagcgac atccggggcc accgttacgt tcgttcccgg atatcgcttc ggactacgtc
     5761 cattatgcgc tcagcgcctg ctccagatcg gcgatcagat cttccttgtc ttcgatgccg
     5821 atcgacagcc gcaccacgtc gggtgccgcg cccgacctga tcttggcggc atcgtcgagc
     5881 tggctgtgcg tggtggaagc cgggtgaatc accagcgagc gggtgtcgcc gacattggcg
     5941 agatgcgaga acagcttcag cttcgacacc aggttgacgc cggcctcata gccgcccttg
     6001 aggctgaagg tgaacaccgc gccggcgccc ttcggcgcat atttacgcgc gaggttgttg
     6061 tatttgtccc cggacagccc ggcgtaattg accgccgcca cggctggatg cgccagcagc
     6121 cactccgcga ccgccttcgc attatcgcaa tgcttctgca tccgcagcgg caacgtttcg
     6181 atgccggtga gaatgagaaa cgcgttgaac ggcgagatcg ccggaccgag atcgcgcaag
     6241 cccagcacgc ggcaggcaat ggcgaatgcg aaattgccga acgtctcctg aatcttgatg
     6301 ccgtgatatt cgggacgcgg ctcgctcagc atcggatatt tgttgtcctt cgaccagtcg
     6361 aaggtgccgg cgtcgacgat gatgccgccg atcgaattgc cgtggccgcc cagaaatttc
     6421 gtcagcgaat gcacgacgat gtctgcgcca tgatcgatcg ggcggatcag atagggcgtc
     6481 gccaaggtat tgtcgacaat caagggcacg ccggccttgc gggcgacggc ggagatcgcc
     6541 tcgatatcgg tgacgctgcc gctggggttg gcgatcgact cgatgaagat cgccttggta
     6601 tgcggcgtta ccgcgcgctc gaagctgccg atttcatcgg gatcggccca caccacgttc
     6661 cagccgaaac tcttgaaggc gtgggtgaac tggttgatcg agccgccata gagttttcgc
     6721 gccgcgatga tttcgtctcc gggcatgagc aactgctgca gcacgacgac ctgcgcggca
     6781 tgtccggatg cgactgcgag tgcggcggtg cctccctcaa gcgcggcaag gcgttcttcc
     6841 agcacggcct gggtaggatt tccgattcgc gtgtaaatgt tcccgaatgc ctgcaacccg
     6901 aacagcgatg cggcatggtc ggcgtcgttg aaaacgaacg acgtggtctg atagatcggc
     6961 gtcgcccgcg cgccggtagt agggtcgggt tgcgcgccgg catgcacggc gagggtcgaa
     7021 aatcccggaa gacgatcggt cattgttttt cctttgtgaa tgcctcatcg agggccgtgc
     7081 ggccgcgtct cgaaggatga gcaatctggt gcgatcttat agcttgagac ggcgcaagcc
     7141 cgcctcgtcc gaatgatcga caatcgcgtc gggggcgtcc gccgtcaaga cggcgcgggg
     7201 ccacagatgg tgttgggagc ggccgtgctt tgcgtcgcca tatttttgaa agaatcgttt
     7261 cgcattggcg tcacgcaagg tcgttcttgt tttttgccgc gcgatcggag gccgtggtct
     7321 cgccgccgcc gacgctcatc cggttcaatg aaagccgcat gccctgggtc ggcatcggcg
     7381 cgcgcttcga acttagcgtt cgcgaattga cgcccatcca cgaaatttcc gaggacagcc
     7441 gtccgtattc gatcttggga caacgattca tgacgacctt gagaccggcg gcctccgcct
     7501 ttgcggccgc ggcatcgtcg cgggcgccaa gctgcatcca gattattctc ggcggcggca
     7561 ccagttccag cgcttcatcg accaccggca tgatgtggct ggagttcctg aagatgtcga
     7621 ccatgtcgac ggggtggcca atatcgcgca gcgacgcgac gaagggcttt cccaaaaggc
     7681 tcttgccgac atgaccggga ttgaccggga tcatgtcgta gccgcgctgc gccagatatt
     7741 tgaaagcaaa
//
LOCUS       DATNOI010000400         2875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_546124,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000400 DATNOI010000000
VERSION     DATNOI010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546124"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..1528
                     /locus_tag="VN968_16030"
     CDS             254..1528
                     /locus_tag="VN968_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HXL30779.1"
                     /translation="MKNITRKSDSSQPAAETAVYLFDDWFDPIEAGVRDRVREFIQAM
                     VEGELDAALMRPRYGRRSQSSSGDADGRVGVTGHRHGHRSRSLMGTFGWTEIAVPRAR
                     LDGPDGQTTEWKSKTLRAYQRRTLAADALIASTYLAGTNTRRVRRALAALFGGAVGKD
                     TVSRVWRKVKADWEAWNARSLAEEPIVRLILDGTVVRVRLDRKATSISLLVVIGVRED
                     GQKVLLALKNMGGETTEAWRAVLDDLINRGLRRPAFLIVDGASGLEKAIAAVWDGVPV
                     QRCTVHKHRNLLAHAPERLHDEITTDYTDMIYAATPEEIETRRKAFIRKWRLRHRPVA
                     DCLEEAGDRLFTFTRLPPSQWRSARTTNAIERLHEEFKRRIKTQTVLPSADTAAMLFW
                     ALLASGQINMRKVDGWQTLATKPIDQQIDLAA"
     gene            2156..>2875
                     /locus_tag="VN968_16035"
     CDS             2156..>2875
                     /locus_tag="VN968_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HXL30780.1"
                     /translation="MTIGITGPRRRAPWRGAVVVLIIAGIVLSICLILLGLTGDFLVD
                     WLWFSTIGYLGVFWTTIAAEGEIFFAVFVATAIILWVNGSIASRIARSPWTQRPADFE
                     WQRTGVATLPDVLEFLRLRLPWPVVVTGGASLLAVLVAWGEVHNWGVFLRFLYQVPYG
                     ASDPLYDKDIGFYLFALPAYVVIKNWMLVTLLLSALFAGAIYWVHGHVEYDAQRRSMS
                     PTAIAHGSVLLGFFFVVKAWSY"
BASE COUNT          536 a          888 c          871 g          580 t
ORIGIN      
        1 aatgtttgcc cggttgcccc tcggtgttcc acttctacat tctgggcctc gacgcgatgt
       61 tcagtcggtc tctcgcaagt gcgcagcccg cgcaatcgtc atgcacagag tggcgctaga
      121 ctatggtcat taagaagcga ccggagacct tcgaggtgcc gtcggcgggg tggtaggaat
      181 tgaggtggcg tagtctccgg ggtgatcaac ctcgaatcgt ccggcgttgg tgcgtcggac
      241 tggagaccac gccatgaaga acattaccag gaagtctgat tcctcgcagc ctgcagctga
      301 gacggcggtt tatttgttcg acgactggtt cgatcccatc gaagctggag tgcgcgatcg
      361 ggtgcgcgaa ttcatccagg cgatggtcga gggcgagctg gatgcggcac tgatgcggcc
      421 gcgatacggc cggcgttcac agtcgtcgag cggtgatgcc gacgggcggg ttggtgtcac
      481 cggccatcgg catggccatc gctcacgttc gctgatgggc acctttgggt ggaccgaaat
      541 cgcggttccg cgcgcccggc tcgacggccc ggacggccag acgacggagt ggaagagcaa
      601 gacactgcgg gcctatcagc gccgcacgct ggccgccgat gcgctgattg caagtacata
      661 cctcgccggc accaacacgc gccgggtccg ccgagcactg gcggcgctgt tcggcggtgc
      721 ggtcggcaag gacacggtga gccgggtgtg gcggaaggtg aaggccgact gggaggcctg
      781 gaacgcccgc tcgctcgcgg aggagccgat cgtgcggctg atcctcgacg gcaccgtggt
      841 gcgggtccgg ctcgaccgca aggccacctc aatatcattg cttgtcgtca tcggcgtgcg
      901 cgaggacggc cagaaagtgt tgctggcgct caagaacatg ggtggagaga ccaccgaggc
      961 ttggcgtgcc gttctcgacg atctgatcaa tcgcggccta cgtcgtcctg ctttcctcat
     1021 cgtggacggc gcgtcggggc tggaaaaggc aatcgccgcg gtctgggacg gcgtgccggt
     1081 tcagaggtgt acggtccata agcacaggaa cctactcgcc cacgcgcccg agcgcctgca
     1141 cgacgaaatc accaccgact acaccgacat gatctacgcc gccacgcccg aggaaatcga
     1201 gacccgccgt aaggccttca tccgcaagtg gcggctgcgt catcgccccg tcgccgactg
     1261 cctggaggaa gccggcgacc gcctcttcac cttcacccgc ctgccgccga gccaatggcg
     1321 aagcgcgaga acaacgaacg cgatcgagcg tctgcatgag gaattcaagc gacggatcaa
     1381 aacccagacc gtgctgccgt cggccgatac tgctgcgatg ttgttctggg cgctgctcgc
     1441 ctcggggcaa atcaacatgc ggaaagtcga tggttggcag acgctcgcca caaaacccat
     1501 cgatcaacag attgacctcg ctgcctgact cggtactctc aaaatgccgg agatcgcgtc
     1561 acctcgaatt ccaacacatc aagcgacggc accattcctt gttgaacatg gttttgcgcg
     1621 acaagcccga gatcaaaacg agggataaac tcattgacgc agaataaatt ctgccatgtg
     1681 agcaattcga taatccgagc ctccgaaagc ggcttggttt cagccccgtg agcctgtctg
     1741 taccagcctt tagcccatat ttcccaaatt aggaatgctc tcgggctgtg acgggggctc
     1801 tttcattttg gccgcccaac ccggctccga cactccccat ttgtctttat ctccaccgca
     1861 cctgaggttt ttacaacccg gacagcggcg cagcagacag cttgaatacc gtcgcccttg
     1921 gggcagagac acgaaccctg tagcgctttc gacgtttcca tctgattgac tttccggatg
     1981 cgggaaacgg gatgatgtag cccccaaaat tacaaagaac cgaacgagtg caagactgtg
     2041 gcaaccgcca tcctctcggc tctcaaaaat gcaggctaca aaattacgtt ggattcaaac
     2101 tgaggcactc gatggcgtag cggtgagcat ctgccgcttc tgctatcatc tagccatgac
     2161 gatcgggatc accggaccca ggcggcgggc gccgtggcga ggcgccgtgg tggtgctcat
     2221 catcgctggc atcgtcctct cgatctgcct gattctgcta gggctcacgg gcgatttcct
     2281 ggtcgactgg ctctggtttt ccacgatcgg ctatttgggc gtcttctgga cgaccatcgc
     2341 cgccgaaggc gaaatcttct tcgccgtctt cgtggcgacc gccatcatcc tttgggtgaa
     2401 cggatcgatc gcgtcccgca tcgcccggtc gccctggacg cagcgtcccg ccgacttcga
     2461 gtggcaacgc acgggcgttg cgaccttgcc ggacgtgttg gaattcctgc gccttcggct
     2521 gccgtggccc gtcgtagtca ccggcggcgc cagcctcctc gccgtgctcg tcgcctgggg
     2581 agaggtccac aactggggcg tcttcctgcg gttcctctat caggtgccgt atggcgcaag
     2641 cgaccccctc tacgataagg acatcggctt ctatctcttt gcgctgcctg cctatgtcgt
     2701 catcaagaat tggatgctgg tcacgctctt gctgagcgcg ctcttcgctg gagcgatcta
     2761 ttgggtccac ggccacgtcg agtacgacgc ccaacgccgg tcgatgtcgc caacagcgat
     2821 cgctcacggc tcggtgctac tcggcttttt cttcgtggtg aaggcctggt cctac
//
LOCUS       DATNOI010000401         2637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_723138,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000401 DATNOI010000000
VERSION     DATNOI010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723138"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="VN968_16040"
     CDS             complement(<1..1054)
                     /locus_tag="VN968_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30781.1"
                     /translation="MSDIALSASVRQNLLSLQSTADLLATTQIRLSTGKKVNTALDDP
                     TNYFTAQGLDSRASDISNLLDGMGNGVQVLQAANTGITSLSKLVDTAKSIATQVLQAP
                     VGYSVKASATSLAPTGAGALGVVGTAADITNAGTNSLQSKTFIFTPATGAATTITISN
                     VVAAGNVNSIDAFNTALAAANLGLTASLASDGSIGLTSTNDNTSQTLTLGAVAAANTI
                     AIGGTDTAVLVTPTGGVAGKPLADAASQTLRSNLVSQYNNIIAQITTTAQDASFNGIN
                     LLNGYDLKLTFNETGKSTLTIKGVTFDAAGLGLASLVAGTDFLDNASAKATMAKITSA
                     SNALRTQASTLGSNLSI"
     gene            1421..1858
                     /gene="flbT"
                     /locus_tag="VN968_16045"
     CDS             1421..1858
                     /gene="flbT"
                     /locus_tag="VN968_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048027 - mRNA 5'-UTR binding [Evidence
                     IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis repressor FlbT"
                     /protein_id="HXL30782.1"
                     /translation="MALKVELKPHERIIIGACVITNTDQRARLLIDGDKIPILREKDI
                     LTPYTADTPAKLVYLAVQLMYISPDPQLNHGTYFNLVRDIVTAVPSAWPIIEGINNQI
                     LNGDLYQALKQARKLVAYEKKLLDHNEASRSKSNAGTEARHSA"
     gene            complement(1912..>2637)
                     /locus_tag="VN968_16050"
     CDS             complement(1912..>2637)
                     /locus_tag="VN968_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HXL30783.1"
                     /translation="DSIQTLLTKIDSITGISTPSTVSGGVITLHTDAAASLTVTSSNP
                     AALAALGFGATVTATLPPLRVSGSPLGAATSLVSGTPANTISWYTGDAGAGSARATAT
                     ARVDQSVTVQYGAQANETAIRSQLQTLAVFAAVTTSATNPNAAAQVQELSLRATQNLL
                     PQPGRQTIQDIQSDFATAQLTMKDATARQKQTKGMLQGIVDQAESVSSDQVASQILAL
                     QTALQASYQTTAMLAQLSLTKFL"
BASE COUNT          517 a          762 c          820 g          538 t
ORIGIN      
        1 cgatcgacag gttcgaaccg agagtcgagg cttgcgtccg caacgcgttc gacgcgctgg
       61 ttatcttggc catcgtcgcc ttggccgagg cgttgtccaa aaagtccgtt ccggcaacca
      121 gcgaagcaag gccaaggccc gccgcgtcga aggtcactcc cttgatggtc agagtggatt
      181 tgccggtctc gttgaaggtc agcttgagat catagccgtt cagcaggttg atgccgttga
      241 acgaagcgtc ctgcgccgtg gtggtgatct gcgcgatgat attgttgtac tggctgacca
      301 ggttggagcg aagggtctgc gaggccgcat cggcgagcgg cttgcctgcg acaccaccgg
      361 tgggcgtaac aaggaccgct gtatcggtac caccgatcgc aatggtgttc gcagccgcga
      421 ccgctcccag tgtaagggtc tggcttgtat tgtcgttggt ggaggtgagc ccgatggacc
      481 cgtcgctggc caatgacgcc gtcaatccaa ggttggccgc cgccagcgca gtattgaaag
      541 cgtcgattga attgacgttg ccggcggcaa ccacgttgct gattgtgatg gtggtggcgg
      601 cgccggtggc aggtgtaaag atgaaagtct tgctttggag cgagttcgta cccgcattgg
      661 ttatatccgc ggccgttccg acaacaccca gcgcgcccgc gccggtcggc gccaggctgg
      721 ttgcggaagc cttcaccgaa taaccgacgg gcgcctgcag cacctgggta gcgatcgact
      781 tcgcggtatc gaccagcttc gacagcgagg tgatgccggt gttggcagcc tgcaacacct
      841 ggacgccgtt gcccatgccg tcgagcaggt tgctgatgtc gctggcgcga ctgtcgagac
      901 cctgtgccgt aaaatagttg gtcgggtcgt caagcgcggt gttgaccttc ttgccggtgg
      961 agaggcggat ctgggtggtg gcgagaagat ccgcggtcga ctgcaacgac agcaggttct
     1021 ggcgaactga ggcggaaaga gcaatatcgg acatttttca tacctttctg gtgtgaaatt
     1081 ggatacgcga gcgtcccaat cttttctgat cgggagaccg gcggacggtg gagccaagcc
     1141 tctaagaaaa tgtgaattgg taccgctgta ctgacctcga cctcacatca gcgccgcgcc
     1201 gacttgcgtt ttcgtcatcg cgccatttga aaatatccct gataatactg cacattcacg
     1261 cgacgtcttg cctcgtttac caggcgttaa ccgcaataag aaaggattgc ggtcacatcg
     1321 gcaaaaaagc atcggcgcgc atctggcgcg accaaagccg gcactcgttc gaactccctg
     1381 tagtgatcgc ccccgcgaga tgcggcaacg gagaacagcc atggctttga aagtcgagct
     1441 caagccgcac gaacggatca tcattggcgc ctgtgtgatc accaacaccg atcagcgtgc
     1501 ccggcttttg atcgacggcg acaagattcc gattctgcgc gagaaggaca tcctgacgcc
     1561 gtacaccgcc gatacgccgg ccaaacttgt ctatctggcg gtgcaattga tgtatatttc
     1621 gccggatccg cagctcaatc acggcactta cttcaatctg gtccgtgata tcgtcaccgc
     1681 cgtgccgagt gcgtggccga tcatcgaggg tatcaacaac caaattctga atggcgacct
     1741 gtatcaggcc ctgaaacaag ccaggaaact cgtcgcctat gagaagaagc tcctggacca
     1801 caatgaagcg agccggagca aatccaacgc tggcaccgaa gcgcggcact ccgcctgagc
     1861 gggatcgctt acatcgccag acgtagttga cgatcgccct aacggcttgg attaaaggaa
     1921 cttggtcagg ctcagttggg caagcatcgc ggtcgtctga taggatgcct gcagggcggt
     1981 ctgcagcgcc aaaatctggc tggccacctg atcggaggaa acgctttcgg cctggtcgac
     2041 gatgccctgc agcattccct tggtttgctt ttgccgggcg gtggcatcct tcatcgtcaa
     2101 ctgcgccgtt gcaaaatccg actgaatgtc ctggatggtt tgccgtcccg gctgcggcaa
     2161 gagattttgc gttgcccgca gactaagttc ctgaacctgc gcggccgcat ttggattggt
     2221 agccgaggtc gtgacggctg cgaaaaccgc gagcgtttga agctgcgaac gaatcgccgt
     2281 ctcgttggcc tgcgccccgt actgcaccgt caccgactga tcgacccgcg ccgtcgcggt
     2341 tgcccgggcg gatccggcgc ccgcgtcgcc ggtgtaccag gagatcgtat tcgccggcgt
     2401 gccgctgacc agcgaggtcg cggcgcccaa tggtgagcca ctaacccgca acggcggcag
     2461 ggtcgccgtc acggtggcac caaagccgag cgccgccaat gcggccgggt tcgaactcgt
     2521 gaccgtcagg ctcgcggcgg cgtcggtgtg cagcgtgatc acgccgccgc tgaccgtcga
     2581 cggggtcgag atgccggtga tcgaatcgat ctttgtcaac agcgtctgga tgctgtc
//
LOCUS       DATNOI010000402         3402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1805676,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000402 DATNOI010000000
VERSION     DATNOI010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805676"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..791)
                     /locus_tag="VN968_16055"
     CDS             complement(51..791)
                     /locus_tag="VN968_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXL30784.1"
                     /translation="MSTVALMLLFLGALASGFVAGLAGFGTALMALGIWLYVLPPSIA
                     VPLVLICSVVAQTSTLPSLWRSFDLRLVWPFLIGGLAGVPLGTMLVAHADPGIFKLSV
                     GILLLVFPPALYFNKPMALSFGGRVADAAIGFAGGILGGLAGLSGVLPTLWASVRGWG
                     KDERRGVFQIFNWTVLAAALCLQAATGLIRIDVVWLALLVMPTTIFGAWLGGRAYRAL
                     SDGNFRDVVLGLLFLSGIGLVWSSLGLR"
     gene            894..1607
                     /locus_tag="VN968_16060"
     CDS             894..1607
                     /locus_tag="VN968_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL30785.1"
                     /translation="MGATFLVCHGAWSAGWAWKKLHPLMSAAGHRLVTPTYTGLGERE
                     HLANPSIDLETHIDDILNVIKYEDLRDIVLIGHSYGGMVATGVADRARDRVVQLIYLD
                     AFVPKDGQSLLDLNEPARQRMLELAKAGDSWRVPPNPTPPDTSQADLGWLTERRVFQP
                     IKCFEMPLKLRGGELTLPRSYIYATRATPADTFRPFAERAKSEAGWRYHELDASHSPN
                     VTAPEALMALLEKIVAEPP"
     gene            complement(1654..2085)
                     /gene="rnpB"
                     /locus_tag="VN968_16065"
     ncRNA           complement(1654..2085)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VN968_16065"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2235..2585
                     /locus_tag="VN968_16070"
     CDS             2235..2585
                     /locus_tag="VN968_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXL30786.1"
                     /translation="MQYLLMIYQNEADYGKIDAATSKKMLEEYGAFTQSIIQSGNFKA
                     GDRLQPTTTATTVRVREGKTLTTDGPFAETREQLGGYYLVEAKDLNAALAIAARIPGA
                     RFGSIEVRPIWVYD"
     gene            2723..>3402
                     /locus_tag="VN968_16075"
     CDS             2723..>3402
                     /locus_tag="VN968_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXL30787.1"
                     /translation="MTPSEIEKIFRDEAGRALATLIRLVGDFDLAEDALQDAFATALE
                     RWPVADPPSNPRAWLVNVGRNKAIDRVRRSIAFRSKQTVLAHELLFNATASDEAADTM
                     ALDDDMLRLILICCHPAFAVEVQVALTLRTVCGLTTAQVARAFLVSEDAMAQRLLRAK
                     QKIRLAGIPYEVPDREALEPRLRGVLAVIYLVFTEGYVATSGEDLMRPDLAREAIRLC
                     RLLDALMP"
BASE COUNT          669 a         1091 c         1054 g          588 t
ORIGIN      
        1 cgctcgcccg cgtacagcgt ttgcgcgctc tggatggcgg ccacgcaagg ctatcgcaac
       61 ccgagactgc tccagaccag accgatcccg gacagaaaca gcaggccaag cacgacgtcg
      121 cggaaatttc cgtcgctcag cgcgcgatag gctcgcccgc cgagccaggc gccgaatatc
      181 gttgtcggca tgaccagcaa tgcgagccaa accacgtcaa tgcggatgag gccggttgcc
      241 gcctgcaggc acaaagcggc tgccagtacg gtccagttga agatctgaaa aaccccgcgc
      301 cgctcatcct tgccccagcc ccgcacgctg gcccacagcg ttggaagcac gccggacaaa
      361 ccggccaggc cgccaaggat tccgccggca aatccgattg ccgcgtccgc tacacggccg
      421 ccaaagctca acgccatcgg cttgttgaaa taaagcgcag gcggaaacac cagcagcagt
      481 atgccgacgc tcaatttgaa tatgccggga tcggcatgcg cgaccagcat cgtgcccaac
      541 ggcacgcccg caaggccgcc gatcaggaat ggccacacca gcctgaggtc gaaggaccgc
      601 caaagcgacg gcagcgttga agtttgcgcg acgaccgaac agatgaggac cagcggcacc
      661 gcgatcgaag gcggcaacac atagagccag atccccagcg ccatgagcgc ggttccgaag
      721 ccggcgaggc cggcgacgaa gccgctggcc agcgcgccga gaaaaagcag catcaacgcg
      781 accgtgctca tcgctccggg tttccatcgt tgaattgatt gctacagagt tggcgaagga
      841 tcggttctgt gccttatagc gatccagccg caatcgcgaa tgaggaaaac atcatgggag
      901 caacctttct cgtctgtcac ggtgcatggt cggcaggatg ggcgtggaag aagctgcatc
      961 cgctgatgag cgccgccggg catcggctgg tgacgccgac ctacaccggg cttggcgagc
     1021 gggagcatct ggcaaacccg tcgatcgatc tcgagaccca cattgacgac atcctgaacg
     1081 tcatcaaata tgaagacctg cgcgacattg tgctgatcgg ccacagctat ggcggcatgg
     1141 tcgcaaccgg ggtagccgac cgcgcacggg accgcgtcgt ccagttgatc tacctcgacg
     1201 cgtttgttcc gaaggacggg cagtctctgt tggatctgaa cgagccggcc cggcagcgga
     1261 tgctggagct cgcgaaggcc ggcgacagct ggcgggtgcc gcccaatccc acgccgcctg
     1321 atacgtcgca agcggatctg ggatggctga ccgagcgccg cgtgttccag ccgatcaagt
     1381 gttttgagat gccgctcaag ctgcgcggcg gcgagctgac gctgcctcgc agctatatct
     1441 acgccacccg tgccacgccg gcggatacct tccgtccgtt cgccgagcgc gccaaaagcg
     1501 aggctggctg gcgttaccac gagctcgacg ccagccattc gccgaacgtc accgcgccgg
     1561 aggcgctgat ggcattgctg gagaagatcg tcgcggagcc gccgtaaaat tggcgagggg
     1621 tccggcgtgt cgcgccgaac ccctcattct tagaatatca gccagcctgt aagccgggtt
     1681 ttgtagggca tcgcctgctt gcgcagacga tacgtgacgg ccattcctct gggacaacgt
     1741 tcgcacgttg cctcgagcaa cctacccgga cggcgagcct gacatcgccc tgcggtgtta
     1801 tcgcttgcgc gaacatgccc gcgttgccgt ccctattcgg ttttgctccc ggtgtggttt
     1861 gccatgccgt ttccgttgcc ggacccgcgg tgcgctctta ccgcaccttt tcacccttac
     1921 tcgccgccgc agcttgcgct gctatggcga gcggttcgtt ctctgtggca ctgtccctgg
     1981 ggtcgccccc gccggacgtt atccggcacc gtatgtcgat ggagcccgga ctttcctctc
     2041 ccgcagcctt tcggccgtag caggagcggc cgtccggccg actgacgcca aaggcatgga
     2101 ggctcaatgt cgccgcgtca agccgataaa acccgcgatg cctgtcaaaa taaattatcc
     2161 tgaagatgtc gttttgcggt ccgcccgttc gaccgggtgt cggcgaatgg cgccggtcaa
     2221 gaggagtgag caaaatgcag tatttgttga tgatctatca gaacgaagcc gactacggca
     2281 aaatcgacgc agcaaccagc aagaaaatgc tggaggaata tggtgcgttc acccaatcca
     2341 tcatccagag cggcaatttc aaggccggcg accggctgca gccgacgacg acggcgacca
     2401 ctgtccgcgt ccgcgagggc aagaccctca ccaccgacgg tccgttcgcc gagacgcgcg
     2461 agcaactcgg cggctactat ctggtcgaag ccaaggatct caacgccgcg ctcgccatcg
     2521 ccgcgcggat tcccggcgcc cgatttggtt cgatcgaggt caggccgatc tgggtttatg
     2581 attgacggcg aagccgtcat tccggggcgc atcgaagatg cgaacccgga atctcgagat
     2641 tccccgatgt gcaattgcac atctgaggtc cacgcgaagg cgcgtgtccc ggaatgacgg
     2701 tgcagtttga acggctgacc caatgacccc cagcgaaatc gaaaaaatat tccgcgacga
     2761 ggcgggtcgg gcgctggcaa cgctgatccg tctggtcggc gatttcgatc tcgccgagga
     2821 cgcgttgcag gacgcctttg ccacggcgct ggaacgctgg cccgtagccg atccgccatc
     2881 caatccgcgc gcttggctgg tcaacgtcgg acgcaacaag gcgatcgacc gggtccgccg
     2941 gagcatcgcg tttcgtagca agcagaccgt actggcgcac gagttgctgt tcaatgccac
     3001 agcctccgac gaagccgccg atacgatggc gctcgacgat gacatgctgc ggctgatcct
     3061 gatttgctgc cacccggcat tcgcggtcga ggttcaggtc gcgctgacgc tgcgcacggt
     3121 ctgcgggctg accaccgcgc aggtcgcgcg cgcgtttctg gtgagcgagg acgcgatggc
     3181 gcagcgcctg cttcgcgcca agcagaaaat ccgcctcgcc ggaattccct acgaggtgcc
     3241 ggaccgcgaa gcgctggagc cgcggctgcg cggcgtgctc gcggtgatct atctcgtctt
     3301 caccgaaggc tatgtggcga catcgggcga agacctgatg cgtccggatc tggcgcggga
     3361 agcgatccgg ctatgtcgtc tgctcgacgc gttgatgccg ca
//
LOCUS       DATNOI010000403         4691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1611284,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000403 DATNOI010000000
VERSION     DATNOI010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611284"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VN968_16080"
     CDS             complement(<1..741)
                     /locus_tag="VN968_16080"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30788.1"
                     /translation="MSCCDETTARVPDTEVSRRALLKGAALVASVIPAARPTESEAQG
                     KQIKLAYCSQLLCGVPYEVARSAGYFKNHGLDVQLVYTRGGNAAMQALVGGAVDYAAT
                     ALDVALQAYANVGADIRRFAVTGRLPLFAVVTAPRTASQIQSIKDLEGRTVGISALGN
                     ADHALTLYLLKQAGADARKVQFATMGVNLLEALRQGQMDAGVVQEPALTLLRRGGARV
                     LMNAMDLEDARHYLGGSFEFMGVAVRARE"
     gene            complement(948..1082)
                     /locus_tag="VN968_16085"
     CDS             complement(948..1082)
                     /locus_tag="VN968_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30789.1"
                     /translation="MLDDLEGAFGSGIREAANAFHISGETLLFGNLTGEKPLVAAAVS
                     "
     gene            1309..2226
                     /locus_tag="VN968_16090"
     CDS             1309..2226
                     /locus_tag="VN968_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30790.1"
                     /translation="MEWRAQSSSLAWSNPLAWWWGVLTLVSGANIAVWFLLYRHLHEQ
                     PTGSLGSTPGIELMLLLCAAYVFGCAFRSFLPRADVQRICLFDTWLSSVVVGRSVATV
                     AEICFAVQWAIILHQLGTMTAADTTLNAAWLLVPLILIAECFSWYAVLTKNYLGNAIE
                     NSIWAVAFLAVGIGLCRLLPEFNGPVRVVLAIAISGIAAYLAFLMTIDVPMYVSRWQA
                     DLADGSKPLGALEGFHDVRTRWVVTHDFAQWKDEIAWMSLYFSAAVWASLALCVVYSL
                     EDHLPRYRTEAAAPAFAGATWLIERGDLT"
     gene            complement(2219..2467)
                     /locus_tag="VN968_16095"
     CDS             complement(2219..2467)
                     /locus_tag="VN968_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30791.1"
                     /translation="MNDYDALRDYLKKQTLPEFVLSFAQIEQIIDAALPRAAHRASWW
                     ETLRSPQEKMPQREACLAGGYIATRLADGKSVKFKRIK"
     gene            complement(2464..3348)
                     /locus_tag="VN968_16100"
     CDS             complement(2464..3348)
                     /locus_tag="VN968_16100"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HXL30792.1"
                     /translation="MKIRKAVFPVAGLGTRVLPATKAMPKEMLTIVDKPLIQHVVDEA
                     REAGIEHFIFVTGRNKGVIEDHFDRVFELDATLEARGKKAEMEILARDQPEAGAMSFT
                     RQQAPLGLGHAVWCARDIVGDEPFAVVLPDELVLHTPGCLAQMIEAAKKLGDKSNLIA
                     VEEVPAHLTHQYGICGVGKRHKHGNMFEVDGMVEKPAKGTAPSNLSITGRYILQPEIF
                     KILETQQRGAGGEIQLTDAMIGLSKTQTFYGVEFKGERHDCGSKAGFLRANIAFAMAR
                     DDLRDGLREEMKKYLDNP"
     gene            3766..>4691
                     /locus_tag="VN968_16105"
     CDS             3766..>4691
                     /locus_tag="VN968_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HXL30793.1"
                     /translation="MASSRTGAAIVVAALLLSGEACAQSSDGPLSLFDNIFTGSLSKG
                     SRTAPSPQRPATSAQAQAAPAASSPAPPWSGEDGASGHPLMTARAIREAAANFENCVV
                     SMWPDAARRNISQQSFQRFTAGLEPDLRIMDLMDSQPEFTRSIWDYLDILVNDNRLAK
                     GREILAKYKPQFDAVEKTYGVDRYTIAAIWGIESSYSTQMGDRSVLQSTATLSCIGRR
                     QKYFKDEFLSALEILHRGDLRPEQLRGSWAGAFGPTQFMPTAFKRYAVDADGDGRRDV
                     VDNPADLIASTANNLKKDGWQPGQSWGYEVVVP"
BASE COUNT          818 a         1476 c         1460 g          937 t
ORIGIN      
        1 ctccctggcg cgtaccgcga cgcccatgaa ttcgaaggaa ccgccgagat agtgcctggc
       61 gtcttcaagg tccatagcgt tcatgagcac gcgcgcgcct ccgcgacgca gcagtgtcag
      121 cgccggctcc tgcactacgc cggcgtccat ttggccctgg cgcaacgctt ccagcaaatt
      181 gacgcccatg gtcgcgaact ggactttccg ggcgtcggcg ccggcctgct tcaacagata
      241 gagcgtcagc gcatgatcgg cattgccgag cgcggagatg ccgaccgttc ttccctccag
      301 atccttgatc gattgaatct ggctggcggt cctgggagcg gtaaccacgg cgaacagagg
      361 gagacgcccc gtcaccgcga agcggcggat gtcggcgcca acattggcat aggcctggag
      421 ggcaacgtcc agcgcggtgg cagcgtagtc gacggcgccg ccgaccagcg cctgcatcgc
      481 ggcgttgccg ccgcgggtgt agacgagctg cacgtcaagc ccatgattct tgaagtaacc
      541 ggccgatcgc gcgacctcgt acggaacgcc gcacagaagc tggctgcaat aggcgagctt
      601 gatctgtttg ccctgagcct cggactcggt cggccgtgcc gctggaatga cgcttgcgac
      661 cagcgccgcc ccctttaaca gcgcacgccg ggacacctcg gtatcgggca cccgggcggt
      721 ggtttcatcg cagcagctca caggagtgtc tccacatcgt tcttccaatg ttttatgccg
      781 atttgacatg tatgcaaggt atggaccttg aactccgcac ctgcatcgag ggggcgaagt
      841 gcgttacgcg acgtcagtgg cactacagcg atttcccggt cagttccgcc ctagcctctg
      901 acaacgctga gcgcaaacga caggtccgtc tagagccgtc ggcgaggtca ggacaccgct
      961 gcagcaacga gaggtttttc tccggtcaaa ttgccgaaca ggagcgtctc gcccgagatg
     1021 tggaatgcat tcgcggcctc gcgtatcccc gacccgaagg cgccttccag atcgtccagc
     1081 atctggatgg gaattgcacg aattcagagc cgggccctcc gcacaatttt gccgccgcga
     1141 agcgaacccg tcgctgtctg tggggctctc gagccgcgcc gctatcacat gccggcagcg
     1201 cccgatagca cctgtgacac gcaaatctcg ctcgcgactg gccccgcctt gtgacagaat
     1261 tgtcacgatg aaatgtcagt tgggttacac gcggagcgtt gcatgagttt ggaatggcgg
     1321 gcgcagtcaa gttccctggc atggtcaaat cccctggcct ggtggtgggg tgtgttaacc
     1381 ttggtcagcg gcgcgaatat cgcggtttgg ttcttgctgt accgccacct ccacgaacag
     1441 ccgactggca gtctcggcag cactccgggc atcgagctca tgctgctgct ctgtgcggcc
     1501 tatgtttttg gctgcgcttt caggtcgttc ctgccgcgcg ccgatgttca gcgtatctgc
     1561 ctgttcgaca cctggctgtc gagcgttgtc gtcggccggt cggtggcaac cgtggccgag
     1621 atctgcttcg cggtgcagtg ggcaattatt ctgcaccagc tgggcacgat gacggcagca
     1681 gacaccacct tgaacgccgc gtggttgctg gtgccgctga tcctgatcgc ggaatgcttc
     1741 tcatggtatg cggtgctgac caaaaattat ctgggcaacg ccatcgagaa ttcgatatgg
     1801 gccgttgcct tcctcgcggt cgggatcggg ctttgccggt tgctgccgga gttcaacggt
     1861 ccggttcgcg tggtcctcgc catcgcgata tcggggattg cagcctatct ggcttttctc
     1921 atgaccatcg atgtcccgat gtatgtgagc cgatggcaag cggaccttgc cgatggcagc
     1981 aagccgctcg gagcactgga gggtttccat gatgtgcgca cgcgttgggt cgtgacgcac
     2041 gactttgccc agtggaagga cgagatcgcc tggatgtcgc tttatttcag cgcggcggtc
     2101 tgggccagcc tcgcgctttg cgttgtctat tcgctcgagg atcatctccc gcgctatcgc
     2161 accgaagcgg cggccccggc gttcgccggg gcgacgtggt tgattgaacg aggcgacctc
     2221 acttgatccg cttgaatttt acgctcttgc cgtccgcgag ccgggtggcg atgtagccgc
     2281 cggcaagaca cgcctcgcgc tgcggcatct tctcctgcgg actgcgcagc gtctcccacc
     2341 acgaggcgcg gtgcgccgcg cgcggcagcg ccgcgtcgat gatctgctcg atctgcgcaa
     2401 agctcagcac gaattccggc agcgtttgct tcttcagata atcgcgcaac gcgtcgtaat
     2461 cgttcacggg ttatccagat atttcttcat ctcttcccgc aagccatcgc gcaggtcgtc
     2521 gcgcgccatg gcgaaggcga tgttggcgcg gaggaaaccg gctttcgaac cgcaatcgtg
     2581 ccgctcgccc ttaaactcga cgccgtagaa ggtttgcgtt ttcgaaaggc cgatcatcgc
     2641 gtcggtgagc tggatctcgc cgccggcgcc gcgctgctgg gtttccagga tcttgaagat
     2701 ctccggctgc aggatatagc gcccggtgat cgacagattc gacggcgcgg tgcccttggc
     2761 cggcttttcc accatgccgt cgacctcgaa catgttgccg tgcttgtggc gcttgccgac
     2821 gccgcagatg ccgtattgat gggtgagatg cgccggcact tcttccaccg cgatcaggtt
     2881 ggatttgtcg ccgagttttt tggcggcctc gatcatttgc gcgaggcagc ccggcgtgtg
     2941 cagcaccagt tcgtccggca gcaccaccgc gaacggctcg tcgccgacga tgtcgcgggc
     3001 gcaccacacc gcgtggccga ggccgagcgg cgcctgctgc cgcgtgaagc tcatggcgcc
     3061 ggcttcgggc tggtcgcggg ccagaatctc catctcggcc ttcttgccgc gggcttcaag
     3121 cgtggcatcg agctcgaaca ccctgtcgaa gtggtcctcg atcacgccct tgttgcggcc
     3181 ggtgacaaag atgaaatgct cgatcccggc ttccctcgcc tcatcgacca catgctggat
     3241 cagcggcttg tcgacgatgg tcagcatttc cttcggcatc gccttggtgg cgggcaggac
     3301 gcgggtgccg agaccggcga ccggaaagac ggctttgcgg attttcatgg ggttgtcgcg
     3361 gtgccttcta ggtgagcatg cgcaaggggc gtttgacggc tttggtaacc ggtttgcagc
     3421 cgggaacaaa gacggttgtg tggtcacgag agggatcgtg aagcgtacag gaactcgggc
     3481 gcatccatgt ctgttccctc cccccttgtg ggggagggtt agggagaggg gtaagccgca
     3541 gacgccggag ttcgcgatac cccctctcca gctttccccc acaagggggg agagagccga
     3601 cctcgccagt tgcagcgctc gcgtttcttt cccgccatcg ctaagtaaaa tctcaaccat
     3661 caaaatctcg cctttgttaa gcgatttgaa accgcgacca ggcctcctga agcgggatat
     3721 gctcgagcag cgcggagtcg gacattgacg cgacggacaa aacaattggc aagcagtcgg
     3781 accggcgcgg ccatagtcgt ggcagcgctg ctattgtccg gcgaggcgtg cgcgcaatcg
     3841 agcgacggcc ccttaagcct tttcgacaac atcttcaccg gctcgctttc gaaaggcagc
     3901 cggactgctc catcgcccca gcggcccgcc acctcggcgc aggcgcaagc ggcgcccgcc
     3961 gcgagcagcc cggcgccgcc ctggagcggc gaggacggcg cctccggcca tccgctgatg
     4021 accgcgcgcg cgatccggga agcggcggcg aatttcgaaa attgcgtcgt ctcgatgtgg
     4081 cccgacgccg cccgccgcaa catctcgcaa caaagttttc agcgctttac cgcagggctg
     4141 gagcccgatc tgcgcatcat ggatttgatg gattcgcagc cggaattcac caggtcgatc
     4201 tgggattatc tcgacatcct cgtcaacgat aaccgcctcg ccaagggccg cgagattctg
     4261 gcgaaataca aaccgcaatt cgacgcggtt gaaaaaacct acggcgtcga ccgctacacg
     4321 attgccgcga tctggggcat cgagtcgagc tactccaccc agatgggcga tcgcagcgtg
     4381 ctgcaatcca ccgcaaccct ttcttgcatc ggccgccgcc agaaatattt caaagacgaa
     4441 ttcctctcgg cgctggaaat cctgcaccgc ggcgatttgc gccccgagca gttgcgcggc
     4501 tcctgggccg gggcgttcgg gcccacgcag ttcatgccga ccgccttcaa gcgctacgcc
     4561 gtggatgccg acggcgacgg ccgccgcgac gtggtcgata atcccgccga cctgattgcg
     4621 tcgaccgcca acaatctgaa aaaggacggc tggcagcccg gccagagctg gggctacgag
     4681 gtcgtggtgc c
//
LOCUS       DATNOI010000404         7572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_789860,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000404 DATNOI010000000
VERSION     DATNOI010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789860"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1094
                     /locus_tag="VN968_16110"
     CDS             207..1094
                     /locus_tag="VN968_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016558810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HXL30794.1"
                     /translation="MKPLFDELTAGFETLPPQLKEAARWVIDHPADVALLTMREQARR
                     AGIPPATLTRLAQRFGLKGYDEIRKLYAEVVRHRPENLRGRAEQLLRRRDSEGDEALI
                     QDIFSALTQHLQALSTSDSIERFTAAAEIIARSKRVFCLGMRSTFSVAYNFHYVRSLL
                     GAASVLVDGPGGIATDTLRMIGPTDVLLAISVKPYTRQTVQAARYAEKRGARVIAVTD
                     SEVSPLAAIASKTLVVRTETPSFFHTMAPAFAAIECLTALVAARRGKQAVQSLAASEQ
                     QLDAFDTYVLTRKKRPKLS"
     gene            1091..2416
                     /locus_tag="VN968_16115"
     CDS             1091..2416
                     /locus_tag="VN968_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXL30795.1"
                     /translation="MTHVLHRQINHAYPTAVSAKGIFIRDAAGKDYIDASGGAAVSCL
                     GHSHPDVLAAMREQLDRLAYAHTSFFTTHVAEELADELVAHAPEGIEHVYFVSGGSEA
                     IEAALKLARQFFVERGETQRRRFIARRQSYHGNTLGALAVGGNEWRRRQFAPLLIETS
                     HVSPCYEYRDRRADESPHAYGERLANELEAKIGELGPETVIAFVAETVVGATLGAVAA
                     VPGYFRRVREICDRHGILLLLDEVMCGMGRTGTLHACEQEGIIPDLMAIAKGLGGGYA
                     PIGAVLASGTIVDTIGKGSGVFQHGHTYIGHPLACAAAIAVQRVIRRDNLLANVRQRG
                     AYLADRLGQRFGNHPNIGDVRGRGLFQAIEIVADRGSKEPFDPALKLHARIKREAMAR
                     GLMVYPMGGTIDGGRGDHVLLAPPFIADAAAIDTIVERLGDAIDASVPD"
     gene            2475..3458
                     /locus_tag="VN968_16120"
     CDS             2475..3458
                     /locus_tag="VN968_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXL30796.1"
                     /translation="MKRNGNLAKRGGIAVAAILALASFSMPAAAQQKFITVGTGGVTG
                     VYYAVGGAICRLMNKDRQKTGIRCSVESTGGSVFNVNAIKSDELDFGMTQSDVQYNAY
                     KGETQFKGNAISDLRVVFAVHPEPFTVLARKEAGVTKFEDFKSKRFNVGNSGSGTRAS
                     MEQLLDAMKWTMKDFSLAAELKADEQGPALCDNKIDGFYYGVGHPSAAIQDPTTTCGA
                     KLISLTGPAIDTLLKNYPYYSVATIPGGMYSGNPNPTTTYGVRALLVTSAKAPDEVVY
                     NLVKAVFENFDEFKKLHPAFATLDPKEMIKAGITAPLHPAAEKFYKEKGWM"
     gene            3520..6135
                     /locus_tag="VN968_16125"
     CDS             3520..6135
                     /locus_tag="VN968_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HXL30797.1"
                     /translation="MERVASQVEDDPAAPRDVDLEELVAESDLGGRRPAGRIGMALSV
                     IAVAWSLFQLWYASPIPYMIGVGVFNDTEARVIHLSFALLLGFMAYPAFKSSPRNRIP
                     LLDWAFAAAAVLCVVYLIYFYRDLAVRPGLPTTADIVVSVVGVVLLLEASRRAEGPWM
                     PIISIVFLFYVFAGPYLPGLFAHKSISVSRAASHFWITSEGVFGVALGVSTAFIFLFV
                     LFGALLEKAGAGNYFIQLAFSLLGQFRGGPAKAGVVSSGMTGMISGSSVANVVTTGTF
                     TIPLMKRVGYTPTQAGAIECAAGVNGQLMPPVMGAAAFLMAEYVGITYADVVRSAFLP
                     AIITYGALFYIVDIEAAKAGMTGLQRTSRRTLLQGATKGLLTICSLIILSGLIYWGLG
                     WTRDLFGDSTSFIALAVVAVAYVGLIANRARHPDLPIDDLRKPLVTVPDFYDVARTGL
                     HYLLPVIVLIWCLLIEEMSPGLAAFWGVLAMAAVVLTQGPMTALFRGERDIGRSWRSG
                     FRDFLGGLENGARNMIAVGIATATAGIIVGTVTLTGVGLVMTEIVELLSAGNFVFMLI
                     LTGLICLVLGSGLPTTASYVVVATLMAPVIVDLAAQNDIAVPLIAAHMFVFYFGLMAD
                     VSPPVGLAAYAAAAIAGADPMKVGWQGMRYENRTALLPFVFIYNPAILMIDIGGPLHF
                     IVVVCCCVLAMGAFVAASQNWLLTRNRWYETVALLVICLTLFRPSFWLDMISAPLTNL
                     PAAQIATEVQKAPKDGALRLRVATQDSSGDTVDKLVRLTLGAGNSVAERLATSGLTLH
                     QVGEEVVIQSVRFGSEARKYGLQPGDKIGAVVVPADRATPYWFTIPALVVLGLIFFAQ
                     RRRRERLRPTMLQPA"
     gene            complement(6190..6390)
                     /locus_tag="VN968_16130"
     CDS             complement(6190..6390)
                     /locus_tag="VN968_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30798.1"
                     /translation="MNHSFYSADRSTHLKIVVVALVMGIAVTGVALSFRPNLDAGSPQ
                     TAGVIKAGKPIAITSSGTSVTR"
     gene            complement(6405..7070)
                     /locus_tag="VN968_16135"
     CDS             complement(6405..7070)
                     /locus_tag="VN968_16135"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXL30799.1"
                     /translation="MFDLDIFKANSRRAAVAIGAIVGAVTLSASAGAGPLPLFSFVTT
                     APQQAAPSQDEGAAELPARLRRQVVSYATREAPGTIIIDTPNTYLYLVLRNGQAIRYG
                     IGVGRDGFTWSGVQSITKKAEWPDWMPPAEMIARQPYLPRYMAGGPGNPLGARAMYLG
                     GTIYRIHGTNAPGTIGTQVSSGCIRLTNEDVADLYSRVNVGTRVIVLPMDRRADTSVA
                     GRG"
BASE COUNT         1383 a         2212 c         2385 g         1592 t
ORIGIN      
        1 cagtcactca actggagatt gtcgtctcat caatctcgga agtcgctggc ctccgaagca
       61 ttctcgacgt cttcgtcgtc gttgccggtc gttgagcgaa acatatgaac tgtttttcca
      121 attagtggaa catgtgttgc atccaggaca ttcctgatct agccttgtgt tcatccgttc
      181 gcgactgtcc gagcaaaaac cttgcgatga aacccctgtt cgatgagctg accgcgggct
      241 tcgaaaccct gcctccgcag ctgaaggagg ccgcgcgctg ggtgatcgat cacccggccg
      301 atgtcgcgct tctcaccatg cgcgaacagg cccgaagggc gggcattccg ccggcgacgc
      361 tgacccggct ggcacagcgc ttcgggttga agggctatga tgaaatccgg aagctttatg
      421 ccgaggtggt ccggcatcgc ccggaaaatc tccgcggccg cgccgagcag ctcttgcgac
      481 ggcgcgacag tgagggtgac gaggcgctga tccaggacat tttctccgcg ctgacccagc
      541 acctgcaagc gctctcgaca tcggattcaa tcgagcgatt tacagcggct gccgaaatca
      601 tcgccagatc caagcgcgtg ttctgcttgg ggatgcgctc gacgttctcg gtcgcttaca
      661 atttccatta tgtgcgctcc ttgctcggcg cggcttcggt actggtcgat ggtcccggcg
      721 gcatcgcaac cgacacgttg cggatgattg gcccaacgga cgtgctgctg gcgatctcgg
      781 taaagccgta tacgcggcag accgtgcagg ctgcgcgata cgccgaaaaa cgtggcgcgc
      841 gggtcatcgc ggtcaccgat agcgaggtct cgccactcgc agcaatagcc agcaaaaccc
      901 tggtcgtgcg aaccgaaacc ccctcgttct ttcacaccat ggcgcctgcc ttcgccgcga
      961 ttgaatgcct gacggcgttg gttgccgcgc gcagggggaa acaggcagtc cagtcgctcg
     1021 cggccagcga acagcagctc gacgcattcg acacctacgt cctgacaagg aagaaacggc
     1081 caaagctgtc atgacccacg tcctgcaccg tcagatcaac catgcctatc cgaccgccgt
     1141 gagtgcgaag ggcattttta ttcgcgacgc ggcaggaaag gactatatcg acgcttccgg
     1201 cggtgctgca gtctcgtgtc ttgggcattc tcatcccgac gtgcttgcgg cgatgcgtga
     1261 gcagctcgac aggctcgcct acgcgcatac aagcttcttc acgacacacg ttgcggaaga
     1321 actcgccgat gaattggtcg ctcacgctcc ggaagggatc gagcacgtct atttcgtcag
     1381 tggcggatcg gaggcgatcg aagccgcgct gaaactggcc cggcagttct ttgtcgagcg
     1441 cggggagacg caacgccggc gcttcattgc ccgcaggcag agctatcacg gtaacacact
     1501 cggtgcgctt gcggttggcg gcaatgaatg gcgccgccgg cagttcgcgc cgcttttgat
     1561 cgagacgtct cacgtttcgc cttgctacga atatcgcgat cgccgtgctg acgaatcgcc
     1621 ccatgcatac ggggagcggc tcgcgaacga gctcgaggcg aagattggag aactcgggcc
     1681 cgaaaccgtg atcgctttcg tcgccgaaac ggtcgtcgga gcgacactcg gcgcggtcgc
     1741 cgcggttccc ggctatttca ggcgcgttcg cgagatctgc gaccggcacg gcattttgct
     1801 tcttctcgac gaggtcatgt gcggtatggg ccgcaccgga acgctccacg catgtgaaca
     1861 ggaaggcatc attcctgatc tgatggcgat cgcgaaaggt ctcggcggcg gctatgcacc
     1921 gatcggcgcc gtgctcgcca gcggcacgat tgtagatacc atcggcaagg gctcaggggt
     1981 gttccagcac ggccacacct atatcggcca tccactcgca tgcgcggcag ctatcgctgt
     2041 ccagcgcgtg ataaggcgtg acaacctgct ggccaatgtg cgccagcgag gtgcatatct
     2101 cgccgaccgt cttggccagc ggttcggcaa ccaccccaac atcggggacg tgcgcggccg
     2161 aggcctgttt caggcgatcg aaatcgttgc ggatcgcggc agcaaggagc cattcgaccc
     2221 cgcgctgaaa ctgcacgcac gcatcaagcg cgaggcgatg gcgcgcggat tgatggtcta
     2281 tccgatgggt ggcacgatcg acggaggccg cggcgatcat gtactgctgg cgccgccgtt
     2341 tatcgccgac gccgcggcga tcgataccat cgtggagcgg ctgggagatg cgatcgatgc
     2401 gtctgttccg gactgaccgg ggctcagctg ctggtgctgc gatagagaga tcagatggaa
     2461 gagggaacct gaacatgaaa agaaatggaa atcttgctaa acgcgggggc attgccgtgg
     2521 cggcaatatt ggcactggcg tcgttctcga tgccggccgc ggctcagcaa aagttcatta
     2581 ccgtcggcac cggtggcgtt acgggtgtct attacgcggt cgggggagcg atctgcaggt
     2641 tgatgaacaa ggatcggcag aagaccggta tccgctgctc cgtggaatcg acgggggggt
     2701 ctgttttcaa cgtcaacgcg atcaagtcgg acgaactcga tttcggcatg acgcagtccg
     2761 acgtgcagta caatgcctac aagggagaaa cccagttcaa gggcaacgcg atctcggacc
     2821 tgcgcgtcgt ctttgccgtc caccccgaac cgttcaccgt gctggcgcgc aaggaggccg
     2881 gtgtcacgaa gttcgaggat ttcaagagca agcgcttcaa cgtcggaaat tctggctccg
     2941 gaacccgtgc atccatggag cagttgcttg atgcgatgaa gtggacgatg aaggatttct
     3001 cgctcgccgc cgaactcaag gccgatgagc agggcccggc gctgtgcgac aacaagatcg
     3061 acggattcta ctatggcgtg ggccatccgt cggcagccat tcaggaccca accacgacgt
     3121 gtggtgcgaa actcatatcg ctcaccggcc cggccatcga cacgctgctg aagaactatc
     3181 cctattattc ggtcgcaacg attccgggtg gaatgtattc cggcaatccc aatcccacca
     3241 cgacctacgg cgttcgcgcg ttgctggtga cgagcgccaa ggcccccgat gaagtggtct
     3301 acaatctcgt caaagcggtg tttgagaatt ttgacgaatt caagaagctg catccggcgt
     3361 ttgcgacact ggacccgaaa gagatgatca aggccggcat cacggcgccg ctgcatccgg
     3421 ctgcggagaa attctacaag gaaaaaggct ggatgtaaca aacgggcgcg gggagagggc
     3481 ggttcgccct ctacatcttg gctgggtgga gggcggcata tggaacgggt cgcatcgcaa
     3541 gtagaggacg atccggcggc gccgcgcgac gtcgatctcg aagaactggt cgccgagtcc
     3601 gatcttggcg ggcgccgccc ggccggccgg atcggcatgg cgctcagcgt gattgccgtc
     3661 gcatggtcgc tgttccagct ctggtatgcc tctccaatcc cctacatgat cggtgtcggt
     3721 gtcttcaacg acactgaggc ccgcgtcatc catctttcgt tcgcgttgct gctcggcttc
     3781 atggcttatc cagctttcaa atcgtcgcca cgcaaccgca tcccgcttct cgattgggca
     3841 ttcgcagcgg cggcagtgct gtgcgtcgtc tatctcatct atttttatcg tgatctcgcg
     3901 gttcgtccgg gtttgccgac gaccgcagac atcgtggtct cggtggtcgg cgtggttctt
     3961 ctgctcgagg cctcgcgtcg cgctgaaggc ccgtggatgc caatcatttc catcgtgttt
     4021 ctgttctacg tgttcgcggg accgtattta cctggtctgt tcgcgcacaa gagtatctcg
     4081 gtttcacgcg cggcttcgca tttctggatc acatcggagg gcgtgttcgg cgttgcgttg
     4141 ggcgtttcga ccgcgttcat ctttctattt gtgctgttcg gcgcgcttct ggagaaggca
     4201 ggcgctggca attatttcat ccagcttgca ttctcgttgc tcggccagtt ccgcggtggc
     4261 ccggcgaagg cgggcgtggt ttcgtcgggc atgacaggca tgatatcggg ctcgtcggtc
     4321 gccaacgtcg tcacgacagg cacgttcacg attccgttga tgaagagggt cggttacacc
     4381 ccgacccagg cgggcgcgat cgaatgtgcc gcaggcgtaa acggtcagct gatgccgcca
     4441 gtgatgggcg cggcggcgtt cctgatggct gaatatgtcg gcatcaccta cgcggatgtg
     4501 gtgcggagcg ctttcctgcc tgccatcatc acctatggag cactgttcta tattgtcgat
     4561 atcgaagccg cgaaggccgg aatgaccgga ttgcagcgca cgagccgtcg aacgctgctg
     4621 caaggggcta cgaaaggtct tctgaccatc tgcagtctca tcatcctgtc cgggttgatc
     4681 tactggggtc tcggctggac cagggacctc ttcggcgatt ctaccagttt cattgcgctt
     4741 gcggttgtgg ccgttgccta tgtcggtctg atcgcaaatc gcgcgcgcca ccccgacctg
     4801 ccgattgatg atttgcgcaa acccctcgtc actgtgccgg atttttacga tgtcgcccgg
     4861 accggactac attacctgct gccggtgata gttctgatct ggtgcttgct gatcgaggaa
     4921 atgtcgcctg gcctggccgc gttctggggc gttctggcga tggcggccgt ggtgctgacc
     4981 caaggcccga tgacggcgct gtttcgcggc gagcgcgaca tcggccgcag ctggcggagc
     5041 ggctttcgcg attttctcgg gggtctcgag aacggcgcgc gcaacatgat cgcagtcggc
     5101 atcgcgaccg cgaccgcggg catcatcgtc gggaccgtga cattgacagg cgtgggcctg
     5161 gtgatgacgg agatcgtcga gctgctctcg gccggcaact tcgtgttcat gctgatcctc
     5221 actggtctga tttgtcttgt gctcggcagc ggcctgccaa ccaccgcgag ctacgtggtt
     5281 gtcgcaacgc tgatggcgcc ggtgattgtc gaccttgccg cgcagaacga catcgccgta
     5341 ccgctgatcg cggcgcatat gttcgtgttc tatttcggtt tgatggccga cgtgtcgccg
     5401 ccggtcggtc tcgccgccta cgcagcggcc gcgattgccg gcgccgatcc gatgaaggtt
     5461 ggttggcagg gcatgcgtta cgaaaaccgc accgcgttgt tgccgttcgt ttttatctat
     5521 aatcccgcga tcctgatgat cgacatcggc ggcccgctgc atttcatcgt ggtcgtttgt
     5581 tgctgtgttc tcgccatggg ggcgttcgtg gccgcgagcc agaattggct gctaacccgc
     5641 aatcgctggt atgaaaccgt agcacttctc gtcatctgcc tgacgctgtt tcgcccctcg
     5701 ttctggctcg acatgatcag cgcgcctctc accaatctgc ccgcggcaca gatcgcgacg
     5761 gaagtgcaga aggcaccgaa ggacggtgcc ttgcgtttgc gcgtggcaac ccaggattcg
     5821 agcggcgata cggtcgacaa gctcgtccgc ctgactcttg gcgccggcaa ttctgtcgcc
     5881 gagcggcttg cgacaagcgg ccttacgctc catcaggtgg gcgaggaggt ggtgatccag
     5941 tccgtgcgtt tcggcagcga ggcgcgcaaa tatggattgc aaccgggtga caagatcggc
     6001 gccgtcgtgg tacctgcgga tcgtgctaca ccctactggt tcacgattcc cgcgcttgtc
     6061 gtgctagggc tcatcttttt cgcccagcgc cggaggcggg agcggttgcg ccccacgatg
     6121 cttcagccag cctagcagcc gtcgctcgag atgcgtgatt ttcaacggac tcgtctattg
     6181 ggcaatcggc tagcgagtaa ccgaggtacc cgaactggta atcgctattg gctttccggc
     6241 cttgatgaca cctgccgtct gcggggagcc cgcgtcaagg ttggggcgga atgacaaggc
     6301 aacgccagtt actgcgatgc ccatcacgag cgcaacgacg acaatcttca gatgcgtcga
     6361 ccggtcggcg ctataaaaag aatggttcac tgtgcgtccc tctctcagcc acgcccggcg
     6421 acagaagtgt cggcccggcg gtccatcggc agcacaatca cccgggtgcc gacgttgacc
     6481 cgcgaataga gatcggcaac gtcctcgttg gtgaggcgga tgcagcccga cgagacctgg
     6541 gtgccgatag tgccgggagc attggtgccg tggatgcggt agatggtgcc accgaggtac
     6601 atggcgcggg cgccgagcgg attgccgggg ccgccggcca tatagcgcgg cagatagggc
     6661 tggcgggcga tcatttccgc agggggcatc caatccggcc attcagcctt cttggtaatc
     6721 gactgcacgc ccgaccaggt gaagccgtcg cggccgacgc cgatgccgta gcggatcgcc
     6781 tgcccgttgc gcagaaccag gtagaggtag gtgttggggg tgtcgatgat gatggtgccc
     6841 ggcgcttcgc gggtggcata gctgaccact tggcgcctca agcgcgccgg tagttcagcg
     6901 gcgccttcgt cctgggacgg cgcggcttgt tgcggcgcgg tggtgacgaa ggaaaacagc
     6961 ggcagcggtc cggcacccgc agatgcggac aatgtgacgg caccaacgat ggcaccaata
     7021 gccactgcgg cgcggcggga attggccttg aaaatatcga gatcgaacat ggtggtcccc
     7081 tgtttgaccg tttgagcccc aagtgcgcct tttcggcaca tcgttatgag agtcagtatc
     7141 tggcgacggt ttccggacgg ttgcgtcagg ggccttaaat ggtttcgtcg acgggagaat
     7201 tatttcatca gagcggggac cgtgacttaa gagatccgga agggggtcac cgcagctcgg
     7261 ccgacgatct cgtgagctag cttccagcgc taaccgaggc tcggtgtttt cctgaaaatt
     7321 gcggatgcaa atggcccttc ggtaaaacgt ttgaaccaaa aaggggccgg gtgagacata
     7381 tccgacagtt gagcgattcc gcaagcgatg ccggccgagg ccggttagct gcaggtcgct
     7441 aaacggtagc tctctatttg gggtgggacc gtgccattat ttcgctattt cactgttgcc
     7501 ggcggagcgc tgcttgcgct gctgtttttg gcgtcatggt gttttcctga aacggaaatc
     7561 atcaaccgca cc
//
LOCUS       DATNOI010000405         6449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_722201,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000405 DATNOI010000000
VERSION     DATNOI010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722201"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VN968_16140"
     CDS             <1..193
                     /locus_tag="VN968_16140"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL30800.1"
                     /translation="IVQKLNHATVEAMKTPAVREKLESAGLKFVSDDRTTPEYLAKFV
                     QSEIAKWAVPIKASGVSVD"
     gene            complement(233..1555)
                     /locus_tag="VN968_16145"
     CDS             complement(233..1555)
                     /locus_tag="VN968_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXL30801.1"
                     /translation="MSGGNVLSAGQAALVLFGVFIGLLIVRVPVAFALGLACLPILLI
                     EPRLSMMMLAQETFNAYNSFILLSVPFFLLTANLMSIGGITDRLVALSRSMVGHWPGS
                     LAQINVVLSVFFAGISGSSTADAASQSKIFIDAQTKEGYDLSFSIAITAVSAVLAVII
                     PPSILMIVWGGLISTSIAAMYLAGIVPGLLIAGAQMLTVHVYAVRRGYPTYPKDSWAE
                     MRCAIWRSIPALMTPFIIVGGILLGWFTATESACVAVLYSVALSTFFYRETGAKELYK
                     ALLDTGRLAGVALFCVGTASAFGWLLAYYKIPEALLANVSTWGMGPIAAGFFIAFCFL
                     VVGCFLDAIPAIVIVGTVLEPLARSVDLHPVQFAIISIVSLAFGLVTPPYGLCLMIAC
                     SIAGVRLRYALKDTIIMLVPMLVVLAALIVWPSVSLFLPRLIVPEMLK"
     gene            complement(1552..2067)
                     /locus_tag="VN968_16150"
     CDS             complement(1552..2067)
                     /locus_tag="VN968_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXL30802.1"
                     /translation="MTEIPMPSNPSLWRRVTAGYAKLLEIMLAACVGILVIPVTLQVI
                     SRYTPFIPSYIWTEEMARFLFVWTIMIGAMVGVREAQHFEVDVWPDLSRRSEAAVRIV
                     ARLGILAMALVFLWAGIEFTRFAWNRTSELADLPLWLIHVAWPVAAFTWIVFAGEQIV
                     DEARILFGTKR"
     gene            complement(2250..3248)
                     /locus_tag="VN968_16155"
     CDS             complement(2250..3248)
                     /locus_tag="VN968_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXL30803.1"
                     /translation="MKRRSVLAGLGATAAAGALGMPSILRAQAPIALNGAVQFNDDHA
                     FTKALVRFEELVQKYYGKPVAFTLHKNSSLGLEKQYFEYMSQGKAVDYGIVSPAHMST
                     FAKAAPFIDAPFVFKGIEHMNKVVEANILAPIADEVATKAEVVLIGYAGGGIRNIFAN
                     KPLRNLADIKGLKVRVQGAPIWSKTFAAAGMSPTVIAYNEVYNAIQNGVIAAGENEAA
                     GVEAMKFYEVAPHLNLTQHAVSIRPICFSVKTLKTLPKDLQDAIMKAGKEAGDFGRQL
                     ESSEEVTKLDALEKAGKLKRVPFEERDAMKKLADPVMATYAKEIGAEGIFEKINVA"
     gene            complement(3571..4533)
                     /locus_tag="VN968_16160"
     CDS             complement(3571..4533)
                     /locus_tag="VN968_16160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30804.1"
                     /translation="MRCVAGLEHPTSGEISIAGRVVSSPARGILVPPRSRDLGMVFQS
                     YAVWPHMTVRQNVIYPLKHRKITRAEAGRKVDEVLELVGLSEYADRPVVALSGGQMQR
                     VALARSIVYRPQLLLLDEPLSNLDAKLRLRLRDDLRVILKQTGMTALYVTHDQAEAVV
                     LGDRIGVMRDGKLLQMGSPDEIYNRPADLFVANFTGATNEIAGTLIERNGASGLVDVG
                     SGQRVEVALLQSIAAGEKIRIALRPENIALGKCDGSNAFNARVLGRRYQGTQTVYDID
                     LFGHRLEVLELGTAARHQVGVETPVSLPREGCWAYRDTGSSPYD"
     gene            complement(4702..5766)
                     /locus_tag="VN968_16165"
     CDS             complement(4702..5766)
                     /locus_tag="VN968_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXL30805.1"
                     /translation="MNNKIVAKIASKIAIKIATMAGIAGLGIASNGTPALAQQEFGSP
                     ELIAAAKGEGKLVYYTANFAEVEQQVIKAFNKRFPEIKIEMVRAPGGQLITRVKTEAA
                     AGKLIADVVDHSDRALMQPLADMFQDYAPPNAADYNPDAQISPNLWPRATLVWSIAYN
                     TELVKDPPKTWMDLTKPEYDRMTGQVFAQSGGTTWTRIMFERLVLGEEYWAKQATTHP
                     VLYPSGAPMADSLVRGEIAMGPLLYNAIYPKQKDGAPVKIFFPPEGAPVNPYATGIPK
                     TAAHPNAAKLFLNWCLSKEGQTFMIKELGHLTSLKEAPVYPEGFDPKVVKVWLPKFDD
                     YVKLHTAWVADWDKVFGYRQ"
     gene            complement(5790..>6449)
                     /locus_tag="VN968_16170"
     CDS             complement(5790..>6449)
                     /locus_tag="VN968_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL30806.1"
                     /translation="YSIFDNPLTLRSIYNAVEVGIITAVVGGTLAFAIGYTIHRTNVA
                     GRRSIDLVSTLPVAIPGLVVGVAYLWAWIGIPGGLYGTIWILALAFIARFMPDTVKAL
                     STSFLQIHRELEEAAWVCGKGMLGTIRTIVLPLARPGVIASMTLLFVLAIRELGSSLF
                     LYTSNTMVMSVLLLDYYEGGNLGKTAAFSLVQTVLLGVLIGGANWLSRGAAQGSVARA
                     G"
BASE COUNT         1334 a         2018 c         1936 g         1161 t
ORIGIN      
        1 gatcgttcaa aagctcaatc atgccaccgt cgaggccatg aaaacgccag cggtgcggga
       61 gaagcttgaa agcgccggcc tcaaatttgt ttccgacgat cgcaccacgc cggagtatct
      121 ggcgaaattc gtgcaaagcg agatcgccaa atgggcggtg cccatcaagg ccagcggcgt
      181 cagcgtcgat tgacgcggca cgtcgcgcca acggcagggg ccggctccgc agctatttca
      241 gcatttcggg aacgatcagg cgcggcagga aaagcgaaac gctcggccag acgattaaag
      301 ccgccagcac gacaagcatc ggcaccagca tgatgatggt atccttgagc gcgtagcgca
      361 gccgcacacc ggcgattgaa cacgcgatca tgaggcacag gccatacggc ggcgtcacca
      421 gcccgaacgc cagcgagacg atcgagatga tcgcgaattg cacgggatgc agatcgaccg
      481 acctcgctag cggctcgagc acggtaccga cgatcacgat ggccggaatg gcatcgagga
      541 agcagccgac aaccagaaaa cagaacgcga tgaagaagcc cgccgcaatc gggcccatgc
      601 cccaggtcga gacgttggcc agcagggctt cgggaatctt gtagtacgcc agcaaccagc
      661 cgaaggcgct tgcggtgccg acgcagaaca gtgcgacccc ggccagacgc ccggtgtcga
      721 gcaaggcctt gtagagttct ttcgctcccg tttcgcgata aaagaaggtc gacagcgcaa
      781 ccgaatacag caccgcgacg caagctgact cggtggccgt aaaccagccc aacaggatgc
      841 cgccgacgat gatgaacggc gtcatcagcg ccgggatcga acgccagatc gcgcagcgca
      901 tctccgccca gctatccttg ggataggtcg gataaccgcg gcggaccgca tagacatgca
      961 cggtcagcat ctgcgcgccc gcgatcagca atcccggcac gatgccggcc agatacatcg
     1021 ccgcgatcga tgtcgagatc agcccgcccc acacgatcat caggatcgac ggcgggatga
     1081 tgaccgcaag caccgccgac accgcggtaa tggcgatgga gaacgaaagg tcataaccct
     1141 ccttggtctg ggcatcgatg aagattttcg actggcttgc cgcatccgcg gtcgaggatc
     1201 cggagatgcc ggcgaagaac accgacagca cgacattgat ctgcgccaac gagccgggcc
     1261 aatgcccgac catcgagcgc gaaagcgcca ccagtcgatc ggtgatgccg ccgatgctca
     1321 tcaggttcgc cgtcagcaga aagaacggca ccgacagcag gatgaaggaa ttgtaggcgt
     1381 tgaaggtttc ctgagcgagc atcatcatcg acaggcgcgg ctcgatcagc aggatcggca
     1441 ggcatgcgag tccgagcgcg aaagcgaccg gcacgcgcac gatcagcagt ccaatgaaaa
     1501 caccaaacaa gacgagcgcg gcctgtccgg cggaaagaac attgccgccg ctcatcgctt
     1561 tgtcccgaac aggatccgcg cctcatcgac aatctgttcg cccgcaaaca cgatccatgt
     1621 aaaggctgcc accggccatg cgacgtgaat gagccagagc ggcaagtcgg ccaactccga
     1681 ggttcggttc caggcaaacc gcgtgaactc gatgcccgcc cacaggaaca ccagcgccat
     1741 cgccaggatg ccaagacgcg ccacgatccg caccgcggct tcgctccgcc gcgacaggtc
     1801 cggccacaca tcgacctcaa aatgctgcgc ctccctgacg ccgaccatcg ccccgatcat
     1861 gatcgtccat acaaacagaa accgcgccat ctcctcggtc cagatgtaag atgggatgaa
     1921 gggtgtgtag cgcgagatca cttgcagcgt aaccggaata accaggatgc cgacgcaggc
     1981 ggccagcatg atctccagca gtttggcata gccggccgtc actcgccgcc acagcgacgg
     2041 gttggatggc atcggtattt cggtcattgg ccccccgcgt catagcagtc atgaagcggg
     2101 gcaagcggtc aggcttgccc cgaaatctcg ttcagcgatc tgctggtcta gagcatgatc
     2161 cggaaaagtg ggtaccggtt tttccgaaaa gatcatgctc aaacaaaaag atagagcggg
     2221 atgacgattc gaggaaaagt catcccgctc taggcgacgt tgatcttttc aaagatgcct
     2281 tcggcgccga tttccttggc gtaggtagcc atcacaggat cggcaagctt cttcatggcg
     2341 tcacgttcct cgaacggtac gcgcttgagc ttgccggcct tttccaacgc atcgagtttg
     2401 gtgacttcct cgctcgactc gagctgccgg ccgaaatcgc cggcctcctt gcccgccttc
     2461 ataattgcgt cctgcaagtc cttgggcaac gtcttgagcg ttttcaccga gaagcaaatc
     2521 gggcggatag acaccgcatg ctgggtcaag ttgaggtgcg gcgcgacctc gtagaacttc
     2581 atcgcctcga cgccggccgc ctcgttttca ccggccgcga ttacgccgtt ctggatggcg
     2641 ttgtagacct cgttataggc aatgaccgtc ggagacatgc cggcggcggc gaacgtcttc
     2701 gaccagatcg gcgcgccctg cacgcgcacc ttgaggccct tgatatcggc caggtttcgc
     2761 agcggcttgt tggcgaatat gttgcggata ccgccgccgg catagccgat caggacgacc
     2821 tcggctttgg ttgcgacttc gtcggcgatc ggcgccagga tgttcgcttc gacgaccttg
     2881 ttcatgtgct cgatgccctt gaacacaaac ggggcatcga taaacggcgc cgccttggcg
     2941 aacgtcgaca tgtgagctgg cgagacgatg ccgtagtcga ccgccttgcc ctgcgacatg
     3001 tactcgaaat actgcttctc gaggccgagc gacgagttct tgtgcagcgt gaaagcgacg
     3061 ggcttgccgt aatacttctg gaccagctct tcgaacctga ccaatgcctt ggtaaaggcg
     3121 tggtcgtcgt tgaactgcac ggcgccgttc agcgcgatgg gcgcctgtgc cctgaggatc
     3181 gatggcatac cgagcgcgcc ggcggccgca gttgccccga gcccggcgag tactgatctt
     3241 cgcttcatgg cgttccctcc tgtgataccc atcccttgtg gccgggattt tgggcgctcg
     3301 cgactgatgg cagtcgcccc tgcagtgctg caatctccaa gcgtatgcaa aaccggcccc
     3361 ctatggaagc caattttgga cggcgcgacc gagcagcgac gatttgacta cacgtatcga
     3421 ctacacgtgg agcctcgcgg caggaatcta tcggcgtatt caaccgaagg ctaacatcac
     3481 atggagactg gattggcaac atcacgcccg gcaatgtcgg gcgcggatca tgctccctta
     3541 ccattcaaaa tttcggagcc atgattgcgg ctaatcgtaa ggcgatgatc ccgtgtcacg
     3601 ataggcccag caaccttccc gcggcaaaga aaccggggtt tcgaccccga cctgatgccg
     3661 ggccgccgtg ccgagttcaa gcacttccag ccggtggccg aacagatcga tatcgtaaac
     3721 cgtctgggtg ccctgatagc ggcggccgag aacgcgcgcg ttgaaggcgt tgctgccgtc
     3781 gcacttgccg agcgcaatat tttccggtcg cagggcgatg cggatttttt ctcccgccgc
     3841 gatcgactgc aacagcgcca cctcgacgcg ctggccgctg ccgacgtcaa ccagtccgga
     3901 cgctccgttg cgctcgatca gcgtgcccgc gatctcattg gtggcgccgg tgaaattggc
     3961 gacgaacaga tcggccggac ggttgtagat ctcgtccggg ctgcccatct gcaacagctt
     4021 gccatcgcgc attacgccga tgcggtcgcc gagcacgacc gcctcggcct ggtcatgggt
     4081 gacgtaaagc gcggtcatgc cggtctgctt gaggatgacg cgcagatcgt cgcgcagccg
     4141 caaccgcaat ttggcatcga ggttggacag gggttcgtcg agcaacaaaa gttgcggccg
     4201 gtagacgatg ctgcgcgcca gcgccacgcg ctgcatctga ccgcccgaca acgccaccac
     4261 cggccggtcg gcatattccg acaggccgac cagttccagc acctcgtcga ctttgcggcc
     4321 ggcttcggcg cgcgtgatct tgcggtgctt gagcggatag atcacgttct gccgcaccgt
     4381 catgtgcggc cagaccgcgt aggactgaaa caccatgccg agatcgcgcg agcgcggcgg
     4441 caccagaatg ccgcgtgcgg gcgacgatac cacgcggccg gcgatgctga tttcgcctga
     4501 tgtcgggtgc tccaaccccg caacgcagcg caacgtcgtg gtcttgccgc accccgaagg
     4561 ccccagcaga cgacaatctc tccggccggc acagcgaagc tgacaccgtc gatggccggc
     4621 cggccgatcg aaaactgctt gtgcaggtcg gtgacgtcga gcggcgccgt catgatttca
     4681 agtgcttcat ggacgggtcg atcactgccg gtagccgaag actttgtccc aatcagccac
     4741 ccaggccgtg tgcagcttca catagtcgtc gaacttgggc agccagacct tgaccacctt
     4801 gggatcgaag ccttccggat agaccggcgc ttctttcaac gacgtgaggt ggccaagctc
     4861 cttgatcatg aaggtctgcc cttctttcga caggcaccag ttgaggaaca gcttcgcggc
     4921 attggggtgt gccgcggtct tcggaattcc cgttgcatag ggattgaccg gcgcgccctc
     4981 gggcgggaag aaaatcttga ccggggcgcc gtctttctgt ttcggataaa tcgcattgta
     5041 gagcaagggg cccatcgcga tttcgccacg caccaatgaa tccgccatcg gcgcacccga
     5101 cggatagagc accggatggg tggtcgcctg cttcgcccag tactcctcgc cgagcaccag
     5161 gcgctcgaac atgatcctcg tccaggtggt gccgccggac tgggcgaaca cctgcccggt
     5221 catccggtcg tattcgggct tggtcagatc catccaggtc ttcggcgggt cctttaccag
     5281 ctccgtgttg taggcgatcg accagaccag ggttgcacgc ggccacaggt tcggcgaaat
     5341 ctgcgcatcg ggattgtagt cggcggcatt cggcggcgca taatcctgga acatgtcggc
     5401 cagcggctgc atcagcgcgc ggtcggaatg atcgaccacg tcggcgatca gcttgccggc
     5461 cgcagcctcg gtcttcacgc gggtgatgag ctggccaccc ggcgcacgca ccatctcgat
     5521 cttgatttcc ggaaaacgct tgttgaacgc cttgatcacc tgctgttcga cctcggcgaa
     5581 attcgccgtg tagtagacca gcttgccttc ccccttggcc gctgcaatca gttcgggcga
     5641 accgaactcc tgctgcgcca acgccggcgt accgttcgac gcgatgccga gaccggcgat
     5701 tccggccatg gtggctatct tgatggctat cttgctagct atcttggcga caatcttgtt
     5761 gttcatggcc aatcctccct tggttttttt tatccggcgc gggcaacgct gccctgggct
     5821 gcgcccctcg acagccagtt cgcgccgccg atcaggaccc cgagcaaaac cgtctgcacc
     5881 agactgaatg cagcggtctt gccgagattt ccgccttcat agtaatcgag cagcagcacc
     5941 gacatcacca tggtattgct ggtgtagaga aacagcgacg agcccagttc gcggatcgcc
     6001 agcacgaaca acagcgtcat cgaagcgatc acgcccggcc gcgccagcgg cagcacgatg
     6061 gttctgatgg tgccgagcat gcccttgccg cagacccagg ccgcttcctc gagctcgcga
     6121 tgaatttgca ggaacgaggt cgacagcgcc ttgaccgtat cgggcatgaa gcgcgcgatg
     6181 aaggcgagcg cgagaatcca gatggtgccg tagagcccgc cgggaatgcc gatccaggcc
     6241 cacaaatagg cgacgcccac cacgaggccg ggaatcgcca ccggcaaggt cgacaccaga
     6301 tcgatgctgc gccggccggc gacattggtg cggtgaatgg tgtagccgat cgcaaaggcg
     6361 agcgtaccgc cgaccacggc ggtgatgatg ccgacctcga ccgcgttgta gatcgatcgc
     6421 agcgtcagcg gattgtcgaa gatgctgta
//
LOCUS       DATNOI010000406        12751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1346037,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000406 DATNOI010000000
VERSION     DATNOI010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12751
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346037"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VN968_16175"
     CDS             complement(<1..1136)
                     /locus_tag="VN968_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isobutyryl-CoA dehydrogenase"
                     /protein_id="HXL30807.1"
                     /translation="MLFALDEDQIAVRDMAREFAAEKIAPHAIRWDEEKHFPVDVMRE
                     AATLGLGGIYIRDDVGGSALTRFDAALIFEALAQGCPTVSAFISIHNMAAWMIDAYGN
                     DTQRRKWLPKLCTMELLASYCLTEPGSGSDAAALRTRAVRDGDHYVLNGQKQFISGAG
                     KGDLYVVMVRTGSDGPGGISTLVIPGDTPGLSFGANERKMGWNAQPTRAVIFENARVP
                     VANRLGDEGIGFKIAMAGLDGGRINIAACSLGGAQCALDKSLAYMKERKAFGKRLDEF
                     QALQFRLADMATELEAARTFVWRAAAALDRKDADATMLCAMAKRFGTDVGFEVANQAL
                     QLHGGYGYLSEYGIEKIVRDLRVHQILEGTNEIMRLIVSRKLIEG"
     gene            complement(1157..2653)
                     /locus_tag="VN968_16180"
     CDS             complement(1157..2653)
                     /locus_tag="VN968_16180"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HXL30808.1"
                     /translation="MRTIGHFIGGREVKGTSGRFADVFEPMTGDVQAKVALASRAELR
                     AAVENAKAAQVEWGATNPQRRARVLMKFLELAQRDYDKLADLLAREHGKTVPDAKGDI
                     QRGLDVVEFACGIPHLMKGEYTEGAGPGIDIYSMRQPLGVVAGITPFNFPAMIPMWKF
                     APAIACGNAFVLKPSERDPGVPMALAALMIEAGLPPGILNVVNGDKEAVDAILDDPDI
                     RAVGFVGSSPIAQYIYERAAATGKRAQCFGGAKNHAIVMPDADMDQTVDALIGAGYGS
                     AGERCMAISVAVPVGKTTADRLMEKLIPRVESLKIGTSIDPSADYGPLVTRAALDRVK
                     NYVEIGIQEGAKLAVDGRSFKMQGYENGFYMGGCLFDHVTKDMRIYKEEIFGPVLSVV
                     RAHDYNEALALPSDHDYGNGVAIFTRDGDAARDFAAKVNVGMVGINVPIPVPIAYYTF
                     GGWKKSGFGDLNQHGPDSIRFYTKTKTVTARWPSGVKEGAEFSIPTMN"
     gene            complement(2830..3468)
                     /locus_tag="VN968_16185"
     CDS             complement(2830..3468)
                     /locus_tag="VN968_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL30809.1"
                     /translation="MRYSKEHKLETHARIVKKASVRLREKGAHGIGVADLMKEAGLTH
                     GGFYAHFDSREALVIEAFAYAMDRGTERWRKLAEATPREKRLATIVGSYLTPLHRDDP
                     GNGCAIPTLGAEIARESPKTRKAFAAKLERMIDMLAEQIPQVPRKAARKRAMAAIATM
                     MGTLVLARVAGNGDFSDEILGAGRDAVLDRAAPLRRVAKKSSPKRAASSARH"
     gene            3685..4455
                     /locus_tag="VN968_16190"
     CDS             3685..4455
                     /locus_tag="VN968_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXL30810.1"
                     /translation="MDMLNPHCGVDRDPRGVVRLTICNAGSLNILGSAVINGVREGLE
                     LLATDPSIRVLVIAGQSEKSMIGGADIKEMAKLDQASAEKFITGLRDLCEAVRAFPAP
                     VIARMPGWCLGGGLEFAAACDFRIAAHDAKFGMPEVRVGIPSVIHAALLPRLIGWGRA
                     RWLIMTAENIDAPTALAWGLVDVVAPGGGLDAAIEKTVAALLECAPEALRAQKALLRQ
                     WEDLPLKESVDLSIGVFGQAFLTGEPQRLMQGFIDRKR"
     gene            4744..5940
                     /locus_tag="VN968_16195"
     CDS             4744..5940
                     /locus_tag="VN968_16195"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXL30811.1"
                     /translation="MATVSDPVVIVSAARTPLGRFMGDLSAFSAHQLGSHVIGAAVER
                     ARLSPERIDEVFMGCVLPAGQGQAPARQAARGAKLPDATGATTINKVCGSGMKATMLA
                     HDIISAGSAEIVVSGGMESMTNAPYLLAKARAGYRTGHDRIIDHMMMDGLEDAYEVGR
                     SMGDFGEATAEAYQFTRADQDAYAMETLSRARKAVEGGAFKAEIIPLTVAEKAGPRTI
                     GNDEIPLKVDPAKIPGLRPAFRANGTITPAASSANADGAAALILTKRSLADRDGLPTL
                     AEIKGHATHSQEPQWFTTAPIPAIRKLLDKVGWNVGDVDLFEINEAFAVVAMAAQRDL
                     GIPREKLNINGGACALGHPIGATGARLIVTLLHALEAQNLKRGVAALCIGGGEATAVA
                     IERIVH"
     gene            6121..7404
                     /locus_tag="VN968_16200"
     CDS             6121..7404
                     /locus_tag="VN968_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL30812.1"
                     /translation="MISNWLSAALARRNIHYGWVMVGVTFLTALITAGTVGAPGVFIA
                     PLQHEFGWTTAEISSALSIRFILFGLMAPFAAALLNRYGLRNVVLSALLIVASALVTS
                     LAMTKLWQLMLLWGVVVGIGTGMTALVLGATVAARWFSARRGLVVGILTASVATGQLV
                     FLPLLARLTERMGWRVALALMCLMLAVAAAAVLLVMRDRPSDLGLRPFGDESTEPLPA
                     PPPNTAPIMAAALGALRDAAKTRVFWILFATFFICGASTNGLVQVHLIPMCLDYGIPQ
                     VQAASLLAAMGIFDFFGTILSGWLSDRHDNRYLLFWYYGLRGLSLLFLPLTDFSFYGL
                     SLFAMFYGLDWIATVPPTVRLTAQRFGPERANLVFGWIFAGHQLGAATAAFGAGLSRT
                     VLATYLPAFFAAGALCIVAALIVLAISRAPKPAVA"
     gene            complement(7482..9041)
                     /locus_tag="VN968_16205"
     CDS             complement(7482..9041)
                     /locus_tag="VN968_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXL30813.1"
                     /translation="MNWKPELDDLARREAFAREMGGVDKVKRQRDQGRLTVRERIDKL
                     VDQKTFHEVGAISGTAEYDKNGELINLTPANCVFGRGKIDGRTVVVVGDDFTVRGGSA
                     DASISTKPLMAEEMAHDFRLPIIRIIEGSGGGGSVKTIETRGAANLPGGIGGTRSYWY
                     TTANMARVPVVGLGLGSVAGLGAARLAASHYSVMTKDSAMFVAGPPVVKRLGQDLTKQ
                     ELGGADIQTRAGGVDHAVDTEEEAFACARRFLSYLPSSVFELPPTLPCDDDPERAEES
                     LIKAVPRNRRQVYKMRPIIEAVVDKGSFFEMAANFGRPVIAGFARLEGRAVLLLASDP
                     FHYGGSWTADACQKVVRFADLAETFHLPVVYLMDCPGFMIGLDAEKAATIRHGVRAMA
                     AVNQTTVPWCTIIVRNAFGVAGVVHQPADRFSMRYAWPSAYWGSLPLEGGIEAAYRAD
                     IDAAEDPKAKLQEIEDRLNKLRSPFRSAERFWVEEVIDPRKTRSLLCEFARLAEPIRT
                     PGLATSMTIRP"
     gene            complement(8971..9945)
                     /locus_tag="VN968_16210"
     CDS             complement(8971..9945)
                     /locus_tag="VN968_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HXL30814.1"
                     /translation="MGRRSERLSKQGMLASDLAGDGDVIQVVSRAFDVLRCFEGHEAR
                     LGNLEISNRCGLPRSTVSRLTHTLTRMGQLVYLPRDQKYRIGPSAVAMSTSMMRGLQL
                     RNLIRLRLQDVAEQLPGTVGFVIPDRFHLVYLEFARAANALGLHESTGSRIAMASTAA
                     GHAYTAALDVDVGDALLAEMEREIPAGAKLLRPRIEANRRFLRERGYVVACGLWSPHI
                     NGLAVPLWSPQYQTFVVVTIGLLSAMYDEKRLHKEVAPRMLELGAAVGAMLEGTEGDI
                     FSNRLERKQLPVPAPNNKIIKTEDTNELEAGTRRPRPARSVRARDGRR"
     gene            10100..10321
                     /locus_tag="VN968_16215"
     CDS             10100..10321
                     /locus_tag="VN968_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HXL30815.1"
                     /translation="MPDIKIVTEVAGRVCALPVEVGGCVGDGDDIAFVEAMKMEIPVA
                     APAAGKLKTILVSLDDVVAEGQVLAILET"
     gene            10379..12238
                     /gene="glsA"
                     /locus_tag="VN968_16220"
     CDS             10379..12238
                     /gene="glsA"
                     /locus_tag="VN968_16220"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HXL30816.1"
                     /translation="MKPAPSASAARATAWAAAPPPLLRFLAACHADFSAETGGAVAAY
                     IPELGKADPEHFGISLATLDGHVYEVGDTRIPFTIQSMSKPFVFALALDTVGAARVES
                     AIGVEPSGDPFNSIRLNADNHPFNPMVNAGAIACSGLIHEAKGDGAFDYIRQALGRFA
                     GRELDIDEAVFASESATGDRNRAIGYLLRNSTVIKDDVSAVLEVYFRQCSVLVTARDI
                     AVMAATLSNRGVNPVTGEQVMTPYAISRTLSVMTSSGMYDYAGEWIYRVGIPAKSGVG
                     GGILAALPARLGLGSYSPRLDRHGNSVRGIKVCEALSRHYDLHMLNRSDDARNSIIAD
                     YNIGKSPSRRSRRPHEQKILAAHHQDVRIIELVGTLTFSSIDYVSRQLAAKPRPQFII
                     FDLRRVAAMTRAGARLLAEEFRELAHLHVIVILSGIKRSSADWKMLKEWTGGISNLRD
                     FYLLDAAIEWAEDQVVYRHGGAIDFHEVTELSEQPLLAGLTPEELAGLAALGTIRSYR
                     PGEKIVAAGEPATSLFFLKSGVVHVTLPDGIRLATLTAGMAFGEMALLEAHRSADVLA
                     DVSATALEVPLREFERFREAHPRTGERIMRNLAQLLADRLIVANTKVDLLTAK"
     gene            complement(12255..>12751)
                     /locus_tag="VN968_16225"
     CDS             complement(12255..>12751)
                     /locus_tag="VN968_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXL30817.1"
                     /translation="HASDRTREILGKNRNANIFRMMAHSPSHFEQYCRLGGAIRHKGE
                     LDPVLRELAITRTGILCEAPYEIVAHKRIGKNVGVTDQQNAALEDWKSATCFSDVQRA
                     ALAFTDEIVGLRRPTDATFDAIAAKLTPAALVELQLSVGFYIMTSKFLETFEIDMQPV
                     AEVV"
BASE COUNT         2190 a         4238 c         3962 g         2361 t
ORIGIN      
        1 ccctcgatca gcttgcgtga cacgatcagc cgcatgattt cgttggtgcc ttccagaatc
       61 tggtgcacgc gcagatcgcg cacgattttc tcgatgccgt attcgctgag gtagccgtag
      121 ccgccatgca gttgcagcgc ctgattggcg acctcgaagc caacgtcggt gccgaagcgt
      181 tttgccatgg cgcacaacat ggtcgcatcc gcgtccttgc ggtcgagcgc cgccgccgcg
      241 cgccacacaa aggtccgcgc cgcctccagt tcggtcgcca tgtcggccag acgaaattgc
      301 agcgcctgga attcgtcgag gcgctttcca aaggccttgc gctccttcat gtaggccagc
      361 gacttgtcga gcgcgcattg cgccccgccg agcgaacagg ccgcgatatt gatgcggccg
      421 ccgtcgagtc cggccatcgc gatcttgaag ccgatgccct cgtcgccgag gcgattggcg
      481 accggcacgc gcgcattctc gaaaatcacc gcgcgggtcg gctgcgcatt ccagcccatc
      541 ttgcgctcgt tggcgccgaa cgaaagtccc ggtgtatcgc ccgggatcac cagcgtcgag
      601 atgccgccgg gcccgtcgct gccagtgcgc accatcacca catagaggtc gcccttgccg
      661 gcgccggaga tgaactgttt ctggccgttg agcacataat ggtcgccgtc gcgcaccgcg
      721 cgggtgcgga gtgccgccgc gtccgatccg gagcccggct cggtcaggca atagctcgcc
      781 aacaattcca tggtgcagag ttttggcagc cacttccggc gctgggtgtc attgccatag
      841 gcgtcgatca tccacgccgc catgttgtgg atcgagatga acgccgacac cgtcggacag
      901 ccctgcgcca gcgcctcgaa gatcagcgcc gcatcgaacc gcgtcagcgc cgaaccgccg
      961 acatcgtcgc ggatgtagat gccgccgagg ccgagcgtcg ccgcctcgcg catcacgtcg
     1021 actggaaaat gcttttcctc atcccaccgg atcgcatgcg gcgcgatctt ttcagcggca
     1081 aattcccgcg ccatatcgcg aaccgcaatc tggtcctcgt cgagcgcgaa cagcatcgcg
     1141 gcttggcttt ctccggtcaa ttcatcgtcg gtatggaaaa ctccgcacct tccttgacgc
     1201 cggagggcca gcgcgcggtc acggtcttgg tcttggtata gaagcggatc gaatccgggc
     1261 cgtgctggtt gagatcgccg aagcctgatt tcttccagcc gccaaacgtg taataggcga
     1321 tcggcaccgg gatcggcacg ttgatgccga ccatgccgac attgaccttg gcggcgaaat
     1381 cgcgcgcggc gtcgccgtcg cgggtgaaga tcgcaacgcc gttgccgtag tcgtgatcgg
     1441 acggcagcgc gagcgcctcg ttgtaatcgt gggcgcgcac caccgagaga acggggccaa
     1501 agatctcttc cttgtagatc cgcatgtctt ttgtcacatg gtcgaacagg cacccgccca
     1561 tataaaaccc gttctcatag ccctgcatct tgaagcttct gccatcgacc gcgagcttgg
     1621 ccccttcctg gatgccgatc tcgacatagt ttttcacgcg atcgagtgcg gccctggtca
     1681 ccagcggacc gtaatccgcc gacggatcga tcgaggtgcc gatcttcagg gattcgaccc
     1741 gcggaatcag tttctccatc agccggtcgg cggtggtctt gccgacgggc accgcgaccg
     1801 aaatcgccat gcagcgctcg ccggccgagc cataacccgc gccgatcaag gcgtcgacgg
     1861 tctggtccat gtcggcgtcc ggcatcacga tggcgtggtt cttggcgccg ccaaaacatt
     1921 gcgcgcgctt gccggtcgcg gcggcgcgct catagatgta ttgcgcgatc ggcgaggagc
     1981 cgacaaaacc caccgcgcgg atatcgggat cgtcgaggat ggcgtcgacc gcttccttgt
     2041 cgccattgac gacgttgagg atgcccggcg gcaggccggc ctcgatcatc agcgccgcca
     2101 gcgccatcgg caccccggga tcgcgctccg acggctttaa gacgaacgca ttgccgcagg
     2161 caatcgcggg cgcgaatttc cacatcggga tcatcgcggg aaaattgaaa ggcgtgatgc
     2221 cggcaacgac gcccaggggt tgccgcatcg aatagatgtc gatgccgggg ccggcgcctt
     2281 cggtatattc gcccttcatc agatgcggaa taccgcaggc gaattcgacc acatcgaggc
     2341 cgcgctggat atcgcccttg gcgtcgggaa cggtcttgcc atgctcgcgc gccaggagat
     2401 cggcgagctt gtcgtagtcg cgctgcgcga gttcaagaaa cttcatcagc acgcgggcgc
     2461 ggcgctgcgg attggtcgcg ccccactcca cttgcgccgc cttggcgttt tcgaccgccg
     2521 cgcgcagctc ggccctggag gccagcgcca ccttcgcctg gacgtcgccg gtcatcggct
     2581 caaacacgtc cgcgaaccgg cccgaggtgc ccttgacctc gcggccgccg ataaaatgcc
     2641 cgattgtgcg catgcgttcc tccaatacct ttgcaatacc tttgcaagtc ccggttttgg
     2701 cggcatcttg cagcctttgc agcgtttggg aagggtttct acgcgcagcg catttgcgaa
     2761 aatgccggca taactcgcgt tttgccgccc cttgcgacca atgtcggatg caattgccga
     2821 aaaatctatt cagtgccgcg ctgacgatgc ggctcttttg gggctggatt tcttcgccac
     2881 ccgcctcagt ggcgccgcgc gatcgagcac ggcgtcgcgc ccggcgccga gaatttcgtc
     2941 ggaaaagtcg ccattaccgg cgacgcgagc cagcaccagc gtccccatca tggtcgcaat
     3001 cgcggccatc gcccgttttc gcgcggcctt gcgcggaact tgcgggatct gctcggcaag
     3061 catgtcgatc atccgttcca gcttggccgc gaatgccttt cgggtcttcg ggctctcgcg
     3121 ggcgatttcc gcccccagcg tcgggatcgc acagccattg ccgggatcgt cgcgatgcag
     3181 cggcgtcaga taggaaccaa cgatcgtcgc cagccgcttc tcccgcggcg tcgcttcggc
     3241 gagcttgcgc cagcgctccg tcccccgatc catcgcataa gcgaaagcct cgatcaccag
     3301 cgcttcccgg gaatcgaagt gggcgtagaa tccgccatgg gtgaggcctg cttccttcat
     3361 caggtcggca acgccaatgc catgcgcgcc cttctcgcga agccgcaccg aagccttctt
     3421 cacgatccgc gcatgggtct cgagcttgtg ttccttagaa taccgcatcg caatcccgca
     3481 agatgtttgc ccataagatg tttaaggtca tataatagca tctgttaata gcatctgttg
     3541 ccgaaaaagc cgcgccttat ttcatttcgc ccgcaacatt acgatcttta catcaagtcc
     3601 gcgcgaggcg ggcaaagcct aacgggcttg acaaggcctc cgatctacca gggaactgat
     3661 gcgccatcaa ttacgagacc acccatggac atgctcaatc cccattgcgg cgtcgaccgc
     3721 gacccgcgcg gcgtggtccg cctgacgatc tgcaacgccg gctcgctcaa catcctcgga
     3781 tcggccgtca tcaacggcgt ccgcgagggg ctggagctac ttgcgaccga cccgtcaatt
     3841 cgggtgctgg tgatcgccgg ccagagcgaa aagagcatga tcggcggcgc cgatatcaag
     3901 gagatggcaa aactcgatca ggcctcggcg gaaaagttca tcaccggcct gcgcgacctc
     3961 tgcgaagccg tgcgggcgtt tccggcgccg gtaatcgcgc ggatgcccgg ctggtgcctc
     4021 ggcggcggcc tcgaattcgc cgccgcctgc gacttccgga tcgccgcgca cgacgccaaa
     4081 ttcggcatgc ccgaggtcag ggtcggcatt cccagcgtga tccatgcggc gctgctgccg
     4141 cggctgatcg gctggggccg cgcccgctgg ctgatcatga ccgcggagaa tatcgatgct
     4201 cccacagcgc tcgcctgggg gctcgtcgat gtggtcgcac cgggtggcgg cctcgatgcc
     4261 gcgatcgaga agacggtcgc ggccctgctg gaatgcgcac cggaagcctt gcgcgcgcaa
     4321 aaagccctgt tgcgccaatg ggaagacctg ccgctaaagg aatccgtcga cctcagcatc
     4381 ggtgtattcg ggcaagcctt cctgaccggc gagccgcagc ggctgatgca ggggtttatc
     4441 gaccggaagc ggtagcaggc ataagaagcc gtcatgcccc gccaacgggt cggcgcaaag
     4501 cgccgcccga tgacaaactc cggcggggca tccagtacgc cgggacgctt gtgggaatcg
     4561 agacggctgc gtttactgga tcatccgcct tcgcggatga tgacagtcgt agctgtcggc
     4621 ccggcctgtc cccttcacga caaaattatc ccttcattgc cgttgtaatt cttgcagcgc
     4681 atcatatgat tatcatcatc caaagacgtc gctttcccga gcgcgaaaat tgaggaacct
     4741 cccatggcca cagtttccga tccggtcgtc atcgtctccg ccgcccgcac cccgcttggc
     4801 cggttcatgg gcgatctctc cgctttcagc gcgcaccagc tcggttccca tgtgatcggc
     4861 gcggcggtcg aacgggccag attgtcgccc gagcggatcg acgaggtgtt catgggctgc
     4921 gtgctgccgg ctggacaggg ccaggccccg gcgcggcagg ccgcgcgcgg cgccaaactg
     4981 ccggatgcga cgggcgcgac caccatcaac aaggtctgcg gctccggcat gaaggccacc
     5041 atgctggcgc atgacatcat cagcgccggc tccgccgaga tcgtggtgtc cggcgggatg
     5101 gagagcatga ccaacgcgcc ctatctgctg gcaaaggcgc gcgccggcta tcgcaccggc
     5161 catgatcgca ttatcgacca catgatgatg gacggcctcg aagacgccta tgaggtcggc
     5221 cgttcgatgg gcgatttcgg cgaagccacc gccgaagcct accagttcac ccgcgccgat
     5281 caggacgcct acgcgatgga gacgctgagc cgcgcgcgca aggcggtgga aggcggcgcg
     5341 ttcaaggccg agatcatacc gctgaccgtc gcagagaagg caggccctcg cacgatcggc
     5401 aatgacgaga ttcccctaaa ggtcgatccc gccaaaattc ccggattgag gccggccttc
     5461 cgcgccaacg gcaccattac gcctgcggcc tcctccgcaa atgccgatgg cgcggcggcg
     5521 ctgatcctga ccaaacgctc gcttgccgac cgcgacggtc ttcccaccct ggccgagatc
     5581 aagggccacg ccacccacag ccaggagccg cagtggttta ccaccgcacc gattcccgcg
     5641 atccgaaagc tgctcgacaa ggtcggctgg aacgtcggcg atgtcgatct gttcgagatc
     5701 aacgaagcgt ttgcggtggt ggcgatggcg gcgcagcgtg acctcggaat tccccgcgag
     5761 aaactgaaca tcaatggcgg cgcctgcgcg ctcggccacc ccatcggcgc caccggcgct
     5821 cggttgatcg tgacgctgct gcatgcgctg gaagcgcaga acctgaaacg cggcgtcgcg
     5881 gccctctgca tcggcggcgg cgaagccacc gccgtcgcca tcgagcgcat cgtccactag
     5941 cgccgcgtca ccgcaccgtg cgtcggccgc gccggcggcg agaaaacttc tcgcccggga
     6001 tagctctgtc gcaaattgga tagggacaga acctggcccg atctaggctt cagcggcgaa
     6061 gccaccgcca tcgcagcgaa ttgttcgccg gcgctcaaca cccaggaaat ttgaaatttc
     6121 atgatctcga attggctgtc ggccgccctc gcccgccgca acatccatta cggctgggtg
     6181 atggtcggcg tgacgtttct caccgcgctg atcaccgccg gtaccgtcgg cgcgcccggc
     6241 gttttcatcg ccccgctgca acatgaattt ggctggacca ccgcggagat ttcctcggcg
     6301 ctgtcgatcc gcttcatcct gttcgggctg atggcgccgt tcgcagccgc cttgctgaac
     6361 cgctacggcc tgcgcaacgt ggtcctgtcg gccttgctga tcgtggcctc ggcgctggtg
     6421 acgtcgctgg cgatgacaaa gctttggcag ttgatgctgt tgtggggcgt cgtggtcggc
     6481 attggcaccg gcatgacggc gctggtgctt ggcgccaccg tcgccgcgcg ctggttttcc
     6541 gcccggcgcg ggctggtggt cgggattctc accgccagcg tggccaccgg gcaattggtg
     6601 ttcctgccgc tactggccag gctcaccgaa cgaatgggct ggcgcgtggc gctggcgctg
     6661 atgtgcctga tgctcgccgt cgcggcggcg gcagtgctgc tggtgatgcg cgatcgtccg
     6721 agcgatctgg gcttgcggcc attcggcgat gagagcacgg agccgttgcc ggcgccgcct
     6781 cccaataccg cgccgatcat ggcggcggcg ctcggtgcgc tgcgcgatgc tgcgaagacg
     6841 cgggtgttct ggatactgtt cgccacgttt ttcatctgcg gcgccagcac caacggcctg
     6901 gtccaggtgc atctgattcc gatgtgcctc gattacggca tcccgcaggt tcaggccgcg
     6961 agcctgctcg ccgccatggg catcttcgat ttcttcggca ccatcctgtc gggctggctg
     7021 tcggatcgcc acgacaaccg ctatctgctg ttctggtatt acggcctgcg tggcctgtcg
     7081 ctgctgttcc tgcccttaac cgatttctcg ttctatggcc tgtcgctgtt tgcgatgttt
     7141 tatggcctcg actggatcgc gacggtgccg ccgacggtgc ggctcaccgc gcaacgcttc
     7201 gggccggagc gcgccaacct ggtgttcggc tggattttcg cgggtcacca actgggcgcg
     7261 gccacggcgg cattcggcgc cggcctgtct cgaaccgtgc tcgcgacata tctgccggca
     7321 ttcttcgccg ccggcgcgct ctgcatcgtc gcggcgctga tcgttcttgc gatctcgcgg
     7381 gctccgaagc cggcggtggc gtgaggcgtg gcgaataggg aatagcgagt agcgaaacct
     7441 gctacactat tcgccactcg ctattcgcca ttcacctcct gctacggcct gatggtcatg
     7501 ctggtcgcaa gccccggcgt gcggatcggc tcggccagcc gggcgaattc gcacaacagc
     7561 gaacgggtct tgcgcggatc gatgacctcc tcgacccaga accgttcggc cgagcgaaac
     7621 ggcgagcgca gcttgttgag acggtcctcg atctcctgca gcttggcttt cggatcctcc
     7681 gcggcgtcga tgtcggcgcg ataggccgcc tcgatgccgc cttccagcgg cagcgaaccc
     7741 caataggccg acggccacgc atagcgcatc gagaaccggt ccgccggctg atgcaccacg
     7801 ccggccacgc cgaaggcgtt gcgcacgatg atggtgcacc aaggcacggt ggtctggttg
     7861 acggcggcca tggcgcgcac gccgtggcgg atggtcgccg ctttttcggc atcgagcccg
     7921 atcatgaagc ccggacaatc catcagatag accacgggca gatggaaggt ttcggcgaga
     7981 tcggcgaacc gcaccacctt ctggcaggcg tctgccgtcc acgacccgcc gtagtgaaag
     8041 ggatcgctgg cgagcagcag cacggcgcgg ccttccagcc gcgcgaagcc ggcgatcacc
     8101 ggccggccga aatttgcggc catctcgaag aacgaaccct tgtcgaccac cgcctcgatg
     8161 atcgggcgca tcttgtagac ctgacgccgg ttgcgcggca ccgccttgat cagcgattcc
     8221 tccgcgcgct ccgggtcgtc gtcgcagggc agcgtcggcg gcagctcaaa gaccgacgac
     8281 ggcagatagg acaggaagcg cctggcgcat gcaaaagctt cctcctcggt atcgaccgcg
     8341 tgatcgacac caccggcacg ggtctggatg tcggcgccgc cgagttcctg cttggtgaga
     8401 tcctggccga ggcgcttcac caccggcgga ccggcgacga acatcgcgga gtccttggtc
     8461 atgaccgaat aatggctcgc cgccagtctc gccgcgccca gtcccgccac cgaaccgagg
     8521 cccaggccca ccacgggcac gcgggccatg ttcgccgtgg tgtaccagta cgagcgggtg
     8581 ccgccgatac cgccgggcag attggccgcc cccctggttt cgatggtttt caccgagccg
     8641 ccgccgccgg agccttcgat gatgcgaatg atcggcagac ggaagtcgtg cgccatctcc
     8701 tcggccatca gcggcttggt ggagatcgag gcatccgcag agccgccgcg cacggtgaaa
     8761 tcgtcgccga ccacgaccac ggtccggcca tcgatcttgc cgcggccgaa cacgcagttc
     8821 gccggcgtca gattgatgag ttcgccgttc ttgtcatatt cggcggttcc ggagatcgcc
     8881 ccaacttcgt ggaatgtctt ctgatcgacc agtttgtcga tgcgctcccg aacggtgagc
     8941 cggccctggt cgcgctgccg cttgaccttg tcaacgcctc ccatctcgcg cgcgaacgct
     9001 tcgcgccggg cgaggtcgtc gagttccggc ttccagttca ttcgtgtcct ccgtcttgat
     9061 gattttatta tttggggccg gtaccggaag ctgtttcctc tccagccggt tgctgaagat
     9121 atctccttcg gtgccctcga gcatggcgcc gaccgccgcg cccagttcca gcatccgagg
     9181 ggccacctcc ttgtgcaacc gcttttcgtc atacatggcc gacagaagac cgatcgtgac
     9241 gaccacgaag gtctgatatt gcggcgacca tagcggcacc gcgagaccgt tgatatgcgg
     9301 gctccacagg ccgcaggcaa cgacatagcc gcgttcgcgt aagaagcggc gattggcctc
     9361 gatgcggggc cgcagcagtt tggcgcccgc cggaatttca cgttccattt ccgccagcag
     9421 ggcgtcgccg acatcgacgt cgagcgcggc ggtgtaggcg tggcccgcgg ccgtgctcgc
     9481 catggcgatg cgactaccgg tgctttcgtg cagaccgagc gcattggcgg cccgggcgaa
     9541 ctcgagatag accagatgaa agcgatccgg aatgacgaac ccgacggttc ccggcagttg
     9601 ctcggcgacg tcctgcagcc gcagccggat caggttgcgc agttgcagtc cgcgcatcat
     9661 cgaggtgctc atagccaccg cactcgggcc gatgcggtac ttctggtcgc gcggcagata
     9721 gacgagctgg cccatccggg tcagcgtgtg ggtcaggcgc gacaccgtcg agcgcggcag
     9781 cccgcagcga ttggaaattt ccagattgcc gagccgcgcc tcgtggccct cgaaacagcg
     9841 caacacgtcg aacgcgcgtg acaccacctg aatgacgtcg ccgtcgccgg caagatcgct
     9901 ggcgagcatt ccctgcttgc tgagccgttc cgagcgtcgt cccatgtggt gccccgccct
     9961 aattccgcta tgcggaataa aattccactt gcagatcagg ctacctcagg caatctgcgg
    10021 ccacaagccg agggcgcccg ccttcatgtg cgcgtattcc gattgcgatg acgcgcggcg
    10081 gctgcccccg gagaatgcca tgccagatat caagatcgtg accgaggtgg cggggcgcgt
    10141 ctgcgcgctt ccggtcgagg tcgggggatg cgtcggcgac ggcgacgaca tcgcattcgt
    10201 cgaagccatg aagatggaaa tcccggtcgc cgccccggca gccgggaagc tcaagaccat
    10261 tcttgtcagt ctcgacgatg tggtcgccga aggacaggtc ctcgccatcc tcgagacctg
    10321 agcgacggcg acgccggcct gttgcgcgcg ccatcattgc gcggcacaac ggcaggcgat
    10381 gaagccagca ccctccgcat ccgccgcccg ggccacggcc tgggccgccg cgccaccgcc
    10441 tttgctgcgg ttcctcgccg cctgtcatgc cgatttttcc gcggagaccg gcggcgccgt
    10501 cgccgcctat atccccgagc ttggcaaggc cgaccccgag catttcggca tcagcctcgc
    10561 caccctcgac gggcatgtct acgaggtcgg cgataccagg atccccttca cgatccagtc
    10621 gatgtcaaaa cccttcgtgt tcgcgctggc gctggatacg gtgggagcgg cgcgggtgga
    10681 aagcgcgatc ggcgtcgaac cgtccggcga ccctttcaat tcgatccgcc tcaatgccga
    10741 caaccatccc ttcaatccga tggtcaacgc cggcgccatc gcctgctccg gcctgatcca
    10801 tgaggccaag ggcgacggcg cattcgacta tatccgtcag gcgctcggcc ggtttgccgg
    10861 gcgtgaactc gacatcgacg aagccgtgtt tgcgtccgaa agtgccaccg gcgaccgcaa
    10921 ccgcgcgatc ggctatctcc tgcgcaactc caccgtcatc aaggatgacg tcagcgcggt
    10981 gctggaagtc tatttccggc aatgctcggt tctcgtgacc gcgcgcgata tcgcggtcat
    11041 ggctgcgacg ctctccaatc gcggcgtcaa tcccgtgacc ggcgagcagg tcatgacccc
    11101 ctatgccatc tcgcggacgc tttcggtgat gacctcttcc ggcatgtacg actacgccgg
    11161 cgaatggatc tatcgggtcg gcatcccggc caagagcggc gtcggcggcg gcattctggc
    11221 ggcgctgccg gcgcggctcg ggctcggcag ctactcgccc cggctcgacc gccacggcaa
    11281 cagcgtgcgc ggcatcaagg tctgcgaagc gctgtcgcga cactacgacc tgcacatgct
    11341 caaccgcagc gacgacgcgc gcaacagcat catcgccgac tacaacatcg gaaaaagccc
    11401 gtcgcggcgc agccgccggc cgcatgagca gaagattctc gccgcccatc accaggatgt
    11461 ccgtatcatc gagctggtgg gcacgctgac attttccagc atcgattacg tttcgcgtca
    11521 gctcgcggcc aagccgcgcc cgcaattcat cattttcgac ctgcgccggg ttgcggcgat
    11581 gacgcgcgcc ggcgcgcggc tgctcgccga ggaattccgc gaactggcgc acctgcatgt
    11641 gatcgtgatt ctgtccggca tcaaacgttc ctccgcggac tggaaaatgc tcaaggaatg
    11701 gaccggcggc atctcgaacc tgcgcgactt ctacctgctc gacgccgcga tcgaatgggc
    11761 ggaagaccag gtcgtctatc gccatggcgg cgcgatcgat ttccatgagg tcaccgaatt
    11821 atccgaacag ccgctgctcg ccggactgac gccggaggaa cttgcaggcc tggccgcgct
    11881 gggcacgatc cggagctacc ggccggggga gaagatcgtc gccgccggcg agccggcaac
    11941 gtcgctgttt ttcctcaaaa gcggggtcgt ccacgtcacg ctgcccgatg gcatccggct
    12001 tgcgacactg accgcgggaa tggcgttcgg agaaatggcg ctattggaag cgcatcgttc
    12061 ggcggacgtc ctggcggatg tatccgctac cgccctcgaa gtgccgctgc gcgagtttga
    12121 gcgctttcgc gaagcgcatc cgcggaccgg cgaacgcatc atgcgcaatc tcgcgcagct
    12181 gctcgccgac cgcctgatcg tggccaacac caaggtggat ttgctgacgg cgaagtagaa
    12241 gcgcgctccg tcgttcaaac cacttcggcc accggctgca tgtcgatctc aaacgtttcc
    12301 agaaatttcg atgtcatgat gtagaaaccg accgagagtt gcagttcgac cagcgctgcc
    12361 ggcgtcagtt tcgccgcgat ggcatcgaac gttgcgtcgg tcggacggcg cagaccgacg
    12421 atttcatcgg taaaggccag cgccgcgcgc tgcacatcgc taaagcaggt cgccgatttc
    12481 cagtcctcca gcgcggcatt ctgctggtcg gtaaccccga cattcttgcc gatacgcttg
    12541 tgcgcaacga tctcgtaagg cgcctcgcat agaattcccg tgcgggtgat ggcgagttcg
    12601 cgcagcaccg gatcgagctc gcccttgtgg cgaatcgcgc cgccgagccg gcaatattgc
    12661 tcaaaatggc tcggcgaatg cgccatcatc cggaagatgt tggcgttgcg gttcttgccc
    12721 aggatttcgc gggtgcggtc gctggcgtgg g
//
LOCUS       DATNOI010000407        22331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_903754,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000407 DATNOI010000000
VERSION     DATNOI010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 22331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22331
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903754"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1175)
                     /locus_tag="VN968_16230"
     CDS             complement(69..1175)
                     /locus_tag="VN968_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXL30818.1"
                     /translation="MFVRSVLSSYYRLLAGLLLALMAIALAGCNEVVAEKAAPSRPVL
                     ISTVHYEPESPERSFVGTIKPRIETDMGFRVPGKVAKRLVEVGQTVDVDQPLATLDEV
                     DLKLQAEQADAEFHAATGVLAQAAAAETRAKDLRAKGWTTDAQLDQAKAAGDEARARL
                     NRAERSVELTKNSLSYATLVADTRGVVTATLIDAGQVVASGQTAVRVARFAEKEAVVA
                     IPETLVGRAKDGVASVTLWSESDKKYAAKLREVAPSADPATRTYLAKFSLPDAGETVS
                     LGMTATLTLADPATTRVARLPLSALFSQGGEPSLYIVDDKGDVALKPVTVKSYETNSV
                     VISDGVDEGAKVVALGVQKLDPAQKVRVVSSLSF"
     gene            complement(1370..2707)
                     /locus_tag="VN968_16235"
     CDS             complement(1370..2707)
                     /locus_tag="VN968_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL30819.1"
                     /translation="MSQSATIITSSPSAKSELETSTIRAISLRLIPFLVLAYFFSYLD
                     RVNLGFAALTMNTELKFSPTIFAWGAGIFFIGYFIFEVPSNLALEKFGASRWIARIMV
                     TWGIISALMALVSGVWSFYALRFLLGVAEAGFFPGIILYLTYWYPAKYRARFLAAFAI
                     AVPVSTVIGAPISGLLLGLDGAMGLKGWQWLFVIEGVPSVVLGVVSWFYLTDRPAKAD
                     WLTAEQKGWLSSKLAGEIAAKQAAAHLTLGQALASPKVITLSLIYFGFVGALYGMQFW
                     LPQIVKAFGFSNAQTGFVTAVPYLFGSVAMVFWARHSDATRERVAHVGLPLLLTAAAL
                     AVCGYMDNPYFTMVALVVAAIGTFCTFALFWTLPTAWLSGSAAAGAIALINSIGNLAG
                     FGGPYLIGWVKEATGSTSTGLLVLAVLPLVGGLLVFLGGHETKAEFAAVGETK"
     gene            3197..3829
                     /locus_tag="VN968_16240"
     CDS             3197..3829
                     /locus_tag="VN968_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30820.1"
                     /translation="MAQFIPANEPIPVILARNSAAAAGAPVGRPAVALIPSYSSTWND
                     FGRYLHANLWVFSASGTATELGFRLMFEGASRTDTHLSQVLGTRDWVDIAEANTLFCS
                     VLDNVESCDTIVKALGFARAVTALRLLGDAVVLRLEGIDSARLALLDTPDFHFGALRQ
                     ESTFVAFRRGGRYLRPQPLITVSDAAISFSLEAKLPSADNGYEITFDFGT"
     gene            3849..4907
                     /locus_tag="VN968_16245"
     CDS             3849..4907
                     /locus_tag="VN968_16245"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXL30821.1"
                     /translation="MSVLIGKNGTGKTQLLLKIIEGLRIQGDTDADQTAQFGPSPSFN
                     RMLVFSSVASDPYPRAIPPWQELDYEYFSMIATLSADRDVLTSALVDCLRDDGKIQFF
                     EGGPFNPRGRLVLLERSMTSLGIWPSIYLPLKIQAEEYDLPSPLVWGGRPFFPLARAR
                     TLNEQRRLLLVQAMDWTQSPLIFGSGDQPRNLSSGEFAMFRFAAQAAGSVERGCIFLF
                     DEPETHLHPNFISEFMDVLHTILTATRSVAIIATHSAYIVREVPRQRVRILSIENREV
                     SIDTPRLQTFGASVDNISQFVFGDTNISHRYQGTLQEWLCSLGPDITIESIVESYGGV
                     MNSETLSYVAQLLRDRVQ"
     gene            4904..5737
                     /locus_tag="VN968_16250"
     CDS             4904..5737
                     /locus_tag="VN968_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXL30822.1"
                     /translation="MISLPPPSAADDETYLADLCQNAGWSVHQGPWLLAYAAYRQHGG
                     NPWAFNPRAFVPDVGDDQRALYDARRSARRLQRIRQTPGLLCCPMCGSPTTGGLDHYL
                     PRSAYPEFSIMAANLVPACTHCNSGVKGNICRGISPERFIHPYFDAFANMPLWQVAFA
                     PPYLAVTFCAVPAPGLNAEQRSRVAFHLAHVLGSQFLLWAQNQWATFPQLVRNTIGGA
                     GTVTTAHVATEARTRLRDAVATTGMNSWLSAFFRGLQEDMLAHVFIATATQPFMATPI
                     A"
     gene            complement(5857..6489)
                     /locus_tag="VN968_16255"
     CDS             complement(5857..6489)
                     /locus_tag="VN968_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HXL30823.1"
                     /translation="MKLYQSNASPNSRRVRIYLAEKGISMPLVPVDLGAREQFSDAYA
                     AINPRRVVPTLVLEDGTAIGEVPAIQRYLEEIHPEPPLLGSTPKAKALVAMWERRVEM
                     EGFASVMEAVRNAVPGLKDRAIAGPHDYEQIPALAERSRQRVGHFYADFNARLADVPL
                     VAGDEFSVADITAVVTVDFATKAAGLPIRGEHTALKRWYDLVSARASMGA"
     gene            complement(6576..7322)
                     /locus_tag="VN968_16260"
     CDS             complement(6576..7322)
                     /locus_tag="VN968_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL30824.1"
                     /translation="MTDLISRTALVTGASRGIGRATARALAAAGARVIVHYGNAAGEA
                     EKLVAEIRASGGHADAVGADLSAPDGAQKLAAEVRELVGARLDILVANAGIATAATIE
                     DQTVEEFDRMFAVNVRAPFFLVQQLLPVLGEGSGVVLMSSLAARASVGLLPAYAATKG
                     AIDTLVKHFAALLGPRGIRVNAVAPGVIDTDMSSFVRSDEGRGYTLGMQALQRIGHPD
                     DVAEVVSFLASDAARWVTGDTIQVGGGSKI"
     gene            complement(7395..8141)
                     /locus_tag="VN968_16265"
     CDS             complement(7395..8141)
                     /locus_tag="VN968_16265"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXL30825.1"
                     /translation="MSHPVVLITGALTGIGRATALAFAQQGARVVVSGRRDDVGEALV
                     AELRALGAEAEFVRADVRHEGDVQNLIDGTVARFGRLDVAVNNAGTEGKPGPVTEQTA
                     DSYAATFDTNVLGVLLSMKHELRVMQAQGAGSIVNLSSTMGQRGAPGASLYTASKHAV
                     EGLTKSAALEAAGFGVRVNAVAPGPIETAMLDRFTGNADRKAGLVAGVPLKRAGRPEE
                     IADTIVFVASSKASFITGQIINVNGGKTAL"
     gene            8317..9225
                     /locus_tag="VN968_16270"
     CDS             8317..9225
                     /locus_tag="VN968_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL30826.1"
                     /translation="MELRHLRYFVAVAEEGSLTVAAQKRLHTAQPSLSRQIHDLELEL
                     GVRLLVRGPRGVELTASGRVFLDHARVALLQVEAATEAARRAAQPARTAFAIGFLTGY
                     EMDWLPAVMGILRAELPSTEVVIHSQDSPDLAAGLMRGKIDLAFLRPEKQAPGLKFRP
                     LRKDPLIVLMPHDHALTARGSVRPQDIAGQTFIGVSAIRAPTLRAVIDDYARRTGLDL
                     KPDHQAENLAMAISLVASTGGVSLLPLYAKNLLPKTVVSRPIQGAPPMVDLVIGYNEA
                     NTSPLLKFLLSRLDELKFRVSRHDGQ"
     gene            9391..10911
                     /locus_tag="VN968_16275"
     CDS             9391..10911
                     /locus_tag="VN968_16275"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HXL30827.1"
                     /translation="MSIATASDFEIAPTRPLLRALISATACVALADWLFYGWQIGISL
                     ALFFGVLGVVAVASNRGRATGNNQIAMSALFVAGLLPLIEDVNILSVIVSTLATALFV
                     IVITTRETLSWQRNLFEAVVTPFRGPFQLAGDLFGALRLMKRRTPQWLSLSSLIGWIV
                     PLSVFLIFLALFSSGNPLIEYRLMQIDLRVLFDVLDLQRMAFWLLVLCAIWPLILRRI
                     RRTPRQEPESSSTVATEPSDFDYLFGVQAMLRSLVLFNALFALQTGLDLVYLWGGATL
                     PDGLSHAEYAHRGAYPLIVTALLAAGFVLIAMRPGGPVEKSRLIRPLVLAWTGQNILL
                     VVSSILRLDLYVAAYSLTYLRLAALIWMGLVAAGLLLMLIQIASQKPNSWLVTANAAT
                     LALVLYGCCFLNAPWVVASYNVDHCREVGGSGPNLDLKYLFSLGPQVLPALEPRLQQI
                     PGLRPYVEGFRFDQDISALRIRPVNWRAWGFRAWRLERYLSNNSYTSSYLSKSDKG"
     gene            10917..11615
                     /locus_tag="VN968_16280"
     CDS             10917..11615
                     /locus_tag="VN968_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL30828.1"
                     /translation="MAHSILVVDDDPHIRDVVRFAFEKAGMLISTAQDGKEALRQFDR
                     NVHELVVLDIGMPEMDGLEVCRQIRKTSDTPILFLSARDEEIDRILGLEIGGDDYVTK
                     PFSPRELVARVNAILRRARTTAAPAVPKAMNHGGLTIDPDGRSVAFHDKRISLTALEF
                     DILKTMLARPGFVFTRELILDAAYAGNIHVADRTIDSHVRNIRAKMAAAGCESAIETV
                     HGVGFRLGRCEALR"
     gene            11612..13231
                     /locus_tag="VN968_16285"
     CDS             11612..13231
                     /locus_tag="VN968_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXL30829.1"
                     /translation="MTDALANTSRRWRPSLSLVVFLVLTSVLSLPLFSLYFLKVYQNE
                     LIQQTEAELIAQSAALSAIFHREVETSIPQGVALGTKVSPVARTASEEPYQPIWPALE
                     LANESVLPPRPRARTPSAPADPAFAALGARMTPDLVATQNVTLAGFRLLDQNGVVIAG
                     REEVGMSLADLEEVSEALQGRFSGVLRVRISKHDQPSLYSISRGTGMRIFTAMPVIVR
                     DQVAGVVYASRTPSNVFKYLYERRRRLMLAMLSMIVPTLLIGFLFHRTITEPMRELVE
                     RTNLVGKGDRDALRPLKRHGTSEFARLSQSFLEMARRLNARSSFISTFATHVSHELKS
                     PLTSIQGAAELLRDDIDAATTPLMSDRDKRKFLDNIIADADRLAKISGRLRDFARAEN
                     PVAVGAARLSGLMAGLRLAFASLDIRTGGDLDTPMRISEENAGIIFSNLADNALRHGS
                     STLDISATRQGNLLQVMVVDNGEGVSPNNRTQIFDSFFTTRRDSGGTGMGLAIVRAML
                     DAHGGAVRLGDCERGTAFELTIPVADEALVS"
     gene            13279..13701
                     /locus_tag="VN968_16290"
     CDS             13279..13701
                     /locus_tag="VN968_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HXL30830.1"
                     /translation="MTMRAGLCLLIIVCGLALRRFGPGLGLPFFFVKYGGSVLWGTMV
                     FFLIAMAAPARSPSAIALISATVAICVELFRLVHTPWLDDFRLTTLGALLIGRVFSPL
                     NMLAYAAGIVSGMWLDRLGISAFASARLSARDAPHSAS"
     gene            complement(13686..14849)
                     /locus_tag="VN968_16295"
     CDS             complement(13686..14849)
                     /locus_tag="VN968_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HXL30831.1"
                     /translation="MPRTIQSHPLTKTQARRIWLRAQRLETSAPFGEGPQATAAAVEH
                     LGYVQIDTINVIERSHHHILWTRIPNYRRADLHQAQSVDRSVFEYWTHALSYVPARDL
                     RFFLPAMKRHRREGHKWLSSVKPADLRKVLRLIRRDGALTIRDIDDDVLTEKEHLWAS
                     RKPSKRALQLAFYTGVLTISERNGMLKTYDLMARHFGWDRPPKPASAGETTAYLLDRA
                     LRAQGVVSLDSICHLDAPSKPSIRRLIQTRVRRNELVPVALEGAGKQEHWARPETLEI
                     PDGGAFEPVHILSPFDPLIIQRKRTELFFGYGHRFEAYVPREKRVFGYFALPVLVGEN
                     IVAAIDLKTDRKSRKLLMQKWSWVDAAPRGARKDLKRRIEEELHRFERFQLAE"
     gene            15173..15367
                     /locus_tag="VN968_16300"
     CDS             15173..15367
                     /locus_tag="VN968_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30832.1"
                     /translation="MRKILIPFVAAALAFSGAAFAQTGGGTSGGAAAKSGTDATAPAA
                     GTAKEGMAPTEKKMKKKKKM"
     gene            complement(15490..15720)
                     /locus_tag="VN968_16305"
     CDS             complement(15490..15720)
                     /locus_tag="VN968_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30833.1"
                     /translation="MPIVIGAIIIAVAILLSTLITAVGTRFVGMENPSEDNVWLIDRL
                     TGSVYKCRAPERGKASCEAEIATGAIGERPKR"
     gene            16264..16908
                     /locus_tag="VN968_16310"
     CDS             16264..16908
                     /locus_tag="VN968_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXL30834.1"
                     /translation="MTFHRLSALKAIALGAALSMTAGLAIAADSNISADQIVTALRAK
                     PLTRGLSTGPQSDAAATARVREIGFVQTLRNRKTRSLSMNEREEIAEIASTKPNIDLD
                     IQFDYNSADISKTSLPAARELGKALSNPDLKGSTFVVAGHTDAIGGEEYNQGLSERRA
                     ETIKRYLTEKYGIAGADLVTVGYGKSKPKDPNAPLDPINRRVQVVNMASKTASN"
     gene            complement(17063..17416)
                     /locus_tag="VN968_16315"
                     /pseudo
     CDS             complement(17063..17416)
                     /locus_tag="VN968_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
     gene            17693..18415
                     /locus_tag="VN968_16320"
     CDS             17693..18415
                     /locus_tag="VN968_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HXL30835.1"
                     /translation="MNSRHGVLYIFALCMAAALAASPARAQTRVGEAAVVKNEVVRVM
                     ASATSQINVGDGVLRDEIVRTGLDSAARLVMADSTNLSLGPGATVKLDRTVFNEEHTY
                     RDIAIRLTTGAFRFVTGHSEKAAYKITTSVATIGVRGTILDILSQRGKTTVVLQEGAS
                     HVCTIGGQCTELTQPGDTAVITSAAGRVTIQRTNNPPWTFAGTCGAAAGLCSTTQFAD
                     ATPSVTPPVDDGSDPTGMLCGR"
     gene            18418..19482
                     /locus_tag="VN968_16325"
     CDS             18418..19482
                     /locus_tag="VN968_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30836.1"
                     /translation="MNKFTRSLIGSATLTALIWLVLVQIQPSPASAGDECFEDCPVTV
                     VVATPPPPPPVTGADSSAGSIGDLGRQRFNQMIINRVLGSVLLGVNEQVNCSDCVSAF
                     GSAGSFSAGIHGRKELTPNLSLLAGIAYTQYSEGGYHITSAPIGAFALRYDFVDWGSS
                     RPFFDVGAILSPSEKARYSRSYMTSLGPVSLESQTHSENYGAYGRAGWVSRISPRDEV
                     AASVEVWQLWQRVNGYSDPTVAFNPFDATIATGTDKTNLVKVGGQWTHLFGNSVEGNI
                     NGGFVQSFANHSGIVASVTGNGTVVTPTLGNQSWFEYGGRLGFRITKGWVADLFVNGT
                     AGPQPVGNTIHGGVGLRVNY"
     gene            complement(19476..21722)
                     /locus_tag="VN968_16330"
     CDS             complement(19476..21722)
                     /locus_tag="VN968_16330"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL30837.1"
                     /translation="MSAPGRSEQTLAAAMAHHRAGRLTDAERLYRIVCDTDPNNARAF
                     RLLGVVAHQLRRPDAASYLGRAVALDPDFAEAHNDRGVILAAGGLLADAISCFERAVT
                     LNPGYGEARTNLGRALRSLGRLDEAVAQFELVLKRAPESPVAHFNLASAFELAGQHPN
                     AEKHYRLAISLRPDFVDAYIHLASLLQNADRLPEALAHAERAVALRPDSAGVRNNLGN
                     ILRALGRRDEAIAQYQTALRIDPGSFMAHYNCGVALRGETRIAEARAHFAQAFALKPD
                     FWQAELALCMAELPAIYQDASDVAERRAAYAGRLARLCADAESAGAPAVLSEAIGSHQ
                     PFYLPYQGRNDRELQALYGRLVCGVMAAKYATAALPAPPSPREPIRLGIVSGFFRQHS
                     NWKIPLKGWLKMLDRTRFQVSGYYTSADRDGETDVAATMCDRFVQGPLSLDAWRRAIL
                     ADAPHVLIFPEIGMDNVSAQLAAQRLAAVQCASWGHPVTSGFPTIDYFISSNLMEPES
                     AADHYSEQLIRLPNLSIYYEPADVPPVAIDRAQLGLRMGAVVYWCCQSLPKYLPQFDE
                     VFARIAAEVPDCQFTFIEFAGGRTVTEMFRGRLRRAFKAGGLDAENHCVFLPRLAPDR
                     FVAAIGQCDVVLDSIGWSGCNSILESLVHNLPIVSFAGAMMRGRHTAAILDMMDISET
                     TARTIEDYVSIAGVLGRNATKRVELSARIAANKHRVYRDRDCIVALEAFLDGAVRNAA
                     RGTGATAQ"
     gene            complement(21753..22067)
                     /locus_tag="VN968_16335"
     CDS             complement(21753..22067)
                     /locus_tag="VN968_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30838.1"
                     /translation="MLMRTIVTLAAILTLGGSPGWANADKAPRRPTRHHHHGYMVRYQ
                     EQPVLPAPYGGGRLVLHPALNIACMTRDRATRALPCDQPVWVYGSPCEVDLGLGRYRS
                     CD"
     gene            complement(22085..>22331)
                     /locus_tag="VN968_16340"
     CDS             complement(22085..>22331)
                     /locus_tag="VN968_16340"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine-peptide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HXL30839.1"
                     /translation="MAIGMPELVTKSLEEYEQAALALARSPQRLIALRQKLQNNRDAS
                     ALFDLPKLTANIEAAYARMWQTWLACQTPAAFSIEGA"
BASE COUNT         4278 a         6949 c         6730 g         4374 t
ORIGIN      
        1 cagaataagc gcggggtggc tgaccgccca gcctgaaaga ttgaagtgtt tcatgacgcc
       61 ctccccgctt aaaacgacaa tgacgagacg accctgacct tctgggccgg atcgagtttc
      121 tgcacaccca gcgcgactac cttggcgcct tcatcgacgc cgtctgaaat gacgacgctg
      181 ttggtctcat aggatttcac ggtgaccggc ttcagcgcca cgtcgccctt gtcatcgacg
      241 atatagagcg agggctcgcc gccctggctg aacagcgccg acaacggcag ccgcgcaacg
      301 cgcgtcgtgg cgggatcggc aagcgtcaac gtcgcggtca tgccgagcga gacggtttcg
      361 ccggcgtcgg gcagtgaaaa tttcgcaaga taggttcgcg tcgcgggatc ggccgacggc
      421 gcgacctcgc gcagcttcgc ggcatatttc ttgtcggatt cggaccacag ggtgacgctg
      481 gcgacgccat ctttcgcccg ccccaccagg gtctccggta tcgcgaccac ggcttccttt
      541 tcggcaaagc gcgcgacgcg aaccgcggtt tgcccggacg ccaccacttg cccggcgtcg
      601 atcagggtcg cggtgacgac gccgcgggta tcggcgacca gcgtcgcata agacagagag
      661 ttcttggtga gttcaaccga gcgctcggcg cgattgagcc gcgcacgcgc ttcatcgccg
      721 gcggctttgg cctgatccag ttgcgcatcc gtggtccagc ccttggcgcg caaatccttg
      781 gcgcgggttt cggcggcggc ggcctgcgcc agcacgccgg tggcggcatg gaattcggca
      841 tcggcctgct cggcctgcag cttcaggtcg acttcgtcga gggtcgccag gggttggtcg
      901 acatcgacgg tctggccgac ttcgaccagc cgttttgcca ccttgccggg aacccggaac
      961 cccatgtcgg tctcaatccg gggcttgatg gtgcccacaa aactgcgttc gggggattca
     1021 ggctcgtagt ggacggtgga gatcaggacc gggcgcgaag gtgcggcttt ctcggcgacc
     1081 acctcgttgc agccggccaa ggcaattgcc atcaaagcta gcaataaacc agctaaaagc
     1141 ctgtagtaac tcgacaaaac ggaacggacg aacatcggac tctccatcgg ctaagcctga
     1201 tgaagactat cgattgttca ctgatgattg tcaatattcg tcatagatca tgatttcgtg
     1261 aacgttaatg aatagttcca gtcgttccgg cgcacgccgg aacccataac ccccgacctc
     1321 agtcattaaa gaaggcctcc gccccggtaa tgacgcgcta gctggcccgt cacttcgtct
     1381 cacccaccgc agcgaattcc gccttggtct cgtggccgcc gaggaaaacc aggagaccgc
     1441 cgaccagcgg caagaccgcg agcaccagca accccgtcga ggtagagccg gtcgcttcct
     1501 tgacccagcc gatcagatag gggccgccga agcccgcgag gttgccgatc gaattgatca
     1561 gcgcgatcgc gccggccgcg gcggatccgg agagccacgc ggtcggcagc gtccagaaca
     1621 gcgcgaaggt gcagaaggtg ccgatcgcgg cgaccaccag cgcgaccatg gtgaagtacg
     1681 gattgtccat gtagccgcag accgcgagcg ccgccgcggt caacagcagc ggcagcccga
     1741 catgtgcgac gcgttcgcgg gtggcgtcgg agtgccgcgc ccaaaacacc atcgcgacgc
     1801 tgccgaacag atacggcacc gcggtgacaa aaccggtctg cgcgttggaa aagccgaagg
     1861 ccttcacgat ctgcggcagc cagaactgca tgccataaag cgcgccgacg aaaccgaaat
     1921 agatcaggct cagcgtgatc acttttggcg aggccagcgc ctgccccagc gtgagatgcg
     1981 ctgcggcctg cttggcggcg atttcgcccg caagcttcga ggacagccac cccttctgct
     2041 cggccgtgag ccagtctgcc ttggccgggc ggtcggtgag atagaaccag gagacgacgc
     2101 cgagcacgac ggagggaacg ccctcgatca cgaacagcca ctgccagcct ttcagcccca
     2161 tcgcgccatc gagcccgagc aggagcccgg agatcggcgc gccgatcacg gtggagaccg
     2221 ggacggcgat ggcaaaggcg gcaagaaacc gggcgcgata ttttgccgga taccaatagg
     2281 tcagatacag aatgatgccc gggaaaaatc cggcctcggc gacgccgagc agaaagcgca
     2341 aggcgtaaaa gctccagacg ccgctgacga gcgccatcaa tgcggagatg atgccccagg
     2401 tcaccatgat gcgcgcgatc cagcggctgg cgccgaattt ctccagcgcc aggttgctcg
     2461 gcacctcgaa gatgaaatag ccgatgaaga agatgccggc gccccaggcg aagatcgtcg
     2521 gcgagaattt cagctcggta ttcatggtca gcgcggcgaa gccgagattg acgcgatcga
     2581 gataggaaaa gaaataggcc agcaccaaaa acggaatcag ccgcaacgag atcgcacgga
     2641 tggtggacgt ctcaagctcg ctcttcgcgc tcggtgaaga ggtgatgatg gtggcggact
     2701 ggctcatgag tttttcccgg acgttttatt cgtaacgatc atccacgtcg tcactttctg
     2761 gtatcggccg gtgtctgcat catcgtggcg gctccctcaa gccttatttt ccaaatgaaa
     2821 acccttggcc acccgtccgg cagcggcgag tatcgcacgc tccgcaaaaa ccgaaggcgt
     2881 tcgtcgcggg cgtttgcaaa aatgctgatc aaggggatgg gataacgtag caaagacgcc
     2941 cgattcgtcg caatcgagac aggcgcagat tcagtccagc gccagaagcg gcatcagccg
     3001 ctacgagatc gcgcgggtcg tgacggcact cagcgcgacc tcgccggtgt tgatagagtt
     3061 aataagtttt cgacttaacg ccgacgattc gccatatgga caatctgtct aattgtgctc
     3121 aatcttccaa tattcgacgt gccgatattt tccatgcaag aacttgttca atgacccccg
     3181 gtcgaagcga aacaatatgg ctcaattcat ccctgccaac gaaccaatac cggtaatcct
     3241 ggctcgaaat tcagccgcag ctgcgggcgc gccagtgggc aggcccgcag ttgcactcat
     3301 tccatcttat tctagcactt ggaacgattt cgggcgctat ttgcacgcca acctgtgggt
     3361 cttttcggca agtggcacag cgaccgaact gggctttcgc ttgatgtttg aaggagcctc
     3421 gcgaaccgat acacatttat ctcaagttct tggaactaga gattgggttg atatcgccga
     3481 ggcaaacacc ctgttttgtt cggtgctcga caatgtcgaa tcgtgcgaca caatcgttaa
     3541 ggcattggga tttgcgcgtg ccgttactgc tttgcgtctg ctcggcgacg cagtggttct
     3601 gcgactcgaa gggattgatt ccgcgcgatt ggcgctcttg gataccccgg actttcattt
     3661 cggggccctg agacaagaaa gtactttcgt ggcgtttcgg cgcggaggcc gctacctccg
     3721 tccccagcct ttgataactg taagcgatgc tgcaataagc ttttcgctcg aagcgaagtt
     3781 gccttcagca gacaacggct acgagataac gtttgatttc ggaacctgac aaattgttgc
     3841 gaaatcgtgt gtccgtctta attggcaaaa acgggacagg aaaaacacag ctattgttga
     3901 agataataga aggtctgcgc attcagggcg acaccgacgc tgatcaaacc gcccaatttg
     3961 gaccgtcgcc atctttcaat cgtatgcttg ttttctcctc cgttgcttct gatccatacc
     4021 cgagagcaat ccctccatgg caagagctcg attacgaata tttctcgatg atagcgaccc
     4081 tctctgccga ccgcgacgta cttactagcg cattggtgga ctgcctacgc gacgacggta
     4141 agatacaatt ctttgaagga gggcccttta atcctcgtgg gcggctcgta ctgctcgaga
     4201 gatctatgac gtctcttggc atttggcctt cgatctatct accattaaaa attcaagccg
     4261 aagaatacga tcttccctca cctctagttt ggggagggcg accatttttc cccctagctc
     4321 gcgctcgcac tctcaatgaa cagcgcagac ttttgctggt tcaggctatg gattggacgc
     4381 agtcgcccct gatattcggt tcaggtgatc aacctcgtaa tttaagtagc ggggagtttg
     4441 caatgttccg gtttgccgcg caagccgcag gttcggtaga aaggggctgc atctttctat
     4501 tcgacgagcc ggagactcat ttacacccta acttcatttc cgaatttatg gatgtgctgc
     4561 atacaatact tacggcgaca cggtcagttg cgatcatcgc aacgcattcg gcttatattg
     4621 ttcgcgaagt acctcgccaa cgcgtcagaa tcttgtcgat tgaaaatcgc gaagtatcga
     4681 tcgatacgcc ccgtctacag acattcggag caagcgtaga taacatttct caattcgtct
     4741 ttggggacac aaatatttct catcgttatc aaggcacttt gcaggaatgg ctttgtagct
     4801 tgggaccaga catcaccatt gaaagtatcg tggagtctta cggcggcgtg atgaacagcg
     4861 aaacattatc ttatgtcgcc caactgcttc gagatcgcgt acaatgattt cgctgccacc
     4921 accatcagct gcagacgacg agacctattt agctgatctc tgtcaaaatg cgggttggtc
     4981 cgtgcatcaa gggccttggc tacttgccta tgccgcttac cgacaacatg gtggaaatcc
     5041 atgggccttc aatccaaggg catttgtgcc tgatgtcggc gacgatcagc gagccttgta
     5101 tgatgcgaga agaagtgcac ggcggctgca acgcattcgg cagacaccag gcctgctgtg
     5161 ttgtccgatg tgtggctcac caacaactgg cgggctcgac cactatttgc cgcgatcagc
     5221 ctacccggaa ttttcaatca tggccgcgaa tcttgtgccg gcatgcaccc actgtaactc
     5281 cggtgtcaaa ggcaatattt gccgtgggat ttcgcctgag cgcttcatcc atccgtactt
     5341 cgacgccttt gcgaacatgc ctttgtggca agtagcgttc gcgccacctt atcttgcggt
     5401 cacgttttgt gcggttccgg cgcctgggtt gaacgcagag caacgctctc gtgtggcgtt
     5461 tcacctcgcg cacgtactgg ggagccagtt tcttttatgg gcgcaaaatc aatgggcaac
     5521 atttccgcag ctggtacgaa atacgatcgg cggcgccggc accgtcacga ccgcgcacgt
     5581 tgccacagag gcacggactc gccttcgtga cgccgtcgcg accacaggaa tgaacagttg
     5641 gctttctgct ttctttcgtg gtctgcaaga agacatgctg gcgcacgtat tcattgctac
     5701 tgcgacccag ccattcatgg cgacgccgat tgcgtaagtt tcgtcggccc ctcacttcgc
     5761 tggctgaaac cgctcgaaac gtcttcctcg atgcggcgtt caggtccttg cgcgcactct
     5821 ttcgcacgcc gtcgctggcg ttctccagcg acaccgctaa gcacccatgc tggcgcgtgc
     5881 agagaccaaa tcataccagc gcttcaatgc cgtatgttcg cccctgatcg gcaagccggc
     5941 cgccttggtg gcgaagtcga cggtgacgac ggccgtgatg tcggcaaccg aaaactcatc
     6001 gcccgccacc aacggcacgt cggcgagccg ggcgttgaag tcggcgtaga aatggccgac
     6061 gcgctgcctg cttcgctcgg cgagcgccgg gatctgctcg tagtcatgcg gtccggcgat
     6121 cgcgcgatcc ttgagaccgg ggacggcgtt gcgaactgct tccatcaccg aggcaaatcc
     6181 ttccatctcg acgcgccgct cccacatcgc gaccagcgct ttggctttcg gcgtgctgcc
     6241 gagcagcggc ggctcgggat ggatctcctc gaggtagcgc tggatggccg gcacttcgcc
     6301 gatcgcggtg ccgtcttcca gcaccaacgt cggaacgacg cggcgcgggt tgatcgcggc
     6361 ataggcgtct gagaactgct ccctggcgcc gagatcgacc ggcaccagcg gcatcgaaat
     6421 ccctttctcg gcgaggtaaa ttctcacccg gcgggaattc ggcgatgcgt tcgattgata
     6481 gagtttcatg tcgacctcca gataaagcct gagacgtacc ggcgggacgt tctcccgaaa
     6541 cggccagcgg cggtgccgcc gcggccgccc tgccctcaaa ttttggagcc gccaccgacc
     6601 tggatggtgt cgccggtcac ccagcgcgcg gcgtcggagg caaggaagct gacgacctcg
     6661 gcgacatcgt cggggtgacc gatgcgctgc agcgcctgca tccccagggt gtagccgcgg
     6721 ccctcgtcgg atcgcacgaa gctcgacatg tcggtgtcga tcacgccggg cgccacggca
     6781 ttgacgcgga tgccgcgcgg gccgagcagc gcggcaaagt gtttcaccag cgtgtcgatc
     6841 gcgcccttgg tcgcggcata ggccggcaac aatccgaccg acgcgcgcgc cgccagcgac
     6901 gacatcagca cgacgccgga accttcgccg agcactggca gcaattgctg caccaggaag
     6961 aacggcgcgc ggacgttgac ggcgaacatg cggtcgaact cttccaccgt ctggtcctcg
     7021 atggtggcag cggtagcgat cccggcattg gcgacgagga tatcgagccg cgcgccgacc
     7081 agttcgcgga cttccgccgc cagtttttgg gcgccatccg gtgcggaaag atcggcgccg
     7141 acggcatcgg cgtgtccgcc ggacgcgcgg atttcggcga ccagcttttc tgcttccccg
     7201 gcggcgttgc cgtagtgaac gatgacgcgc gcgccggcgg cggccagcgc gcgggcggtg
     7261 gcgcggccga tgccgcgcga ggcgccggta acaagtgcgg ttctgctgat gaggtcggtc
     7321 atggctgttc tccggtccgg aatgagaaat gtttggaaga ttttacgaag cggtcttgtt
     7381 acgaagcagt cttgttacaa agcggtcttg ccgccattga cgttgatgat ctggccggta
     7441 atgaacgacg ccttgctgga ggcgacgaac acgatggtgt cggcgatctc ttcgggccgg
     7501 ccggcgcgtt tcagcggcac gcctgccacc agaccggctt tccggtcggc attgccggtg
     7561 aagcggtcga gcatcgcggt ctcgatcggc cccggcgcga ccgcattgac acggacgccg
     7621 aacccggcgg cttccagcgc cgccgatttt gtcagccctt cgacggcgtg cttgctcgcg
     7681 gtgtagagcg aggcgccggg cgcgccgcgc tggcccatgg tcgacgacag attgacgatg
     7741 ctgccggctc cctgcgcctg catcacccgc agctcgtgct tcatgctcaa cagcacgccg
     7801 agcacgttgg tgtcgaaggt ggcggcgtag ctgtcggcgg tctgctcggt caccgggccg
     7861 ggcttgccct cggtgccggc gttgttgacg gcgacgtcga ggcggccgaa ccgggcgacc
     7921 gtcccgtcga tcaggttttg cacgtcgcct tcgtggcgga cgtcggcgcg gacgaattcc
     7981 gcttccgcgc caagcgcccg caactcggcg accagcgctt cgccgacatc atcgcggcgg
     8041 ccggaaacga cgacgcgggc gccttgctgc gcgaaagcga gcgcggtggc gcggccgatg
     8101 ccagtcagcg cgccggtgat cagtacaacg ggatggctca tttggtttct ccttcaaggc
     8161 agctatgcct gtcgagcctt gctgtgagtt ccagttgggg agcggcggcg cggcgcgact
     8221 gcgctttatt gctgcaaggc aggttatcta gatctcgctg gtgtcccgga gaaagacttt
     8281 gtaaggtcaa agccatactt ttgaggaatg gcctggatgg agctgcgaca cctccggtac
     8341 ttcgtcgccg tggccgaaga aggcagcctg accgtggctg cgcaaaagcg gctgcacacc
     8401 gcgcagccgt cgctgagccg gcagattcac gatctcgaac tggagcttgg ggtgcggttg
     8461 ttggtccgcg gtccgcgcgg ggtcgaactg accgcatctg gaagagtgtt tctcgatcac
     8521 gcgcgggtgg cgctgctgca ggtcgaggcg gccaccgagg cggcgcgacg ggcggcgcaa
     8581 ccggcgagga cagccttcgc catcggtttc ctgactggtt acgagatgga ttggcttccg
     8641 gctgtgatgg gcatcctgcg cgccgaatta ccctctaccg aagtcgtcat ccatagccag
     8701 gactcgccgg atctcgcagc gggtctcatg cgcgggaaaa tcgacctcgc ctttctgcgc
     8761 ccggaaaagc aggcgcccgg gctgaaattc aggccactgc gaaaggatcc gctgatcgtc
     8821 ctgatgccgc acgatcacgc cctgaccgcg cgcggctctg ttcggccaca ggatatcgcc
     8881 ggccagacct tcatcggcgt atccgcgatc cgcgcgccga cgctgcgggc tgtcatcgac
     8941 gactacgcca ggcgcaccgg ccttgatctc aagcccgacc accaggccga gaatctggcg
     9001 atggcgattt cgctggtggc gtccaccggc ggcgtcagcc tgcttccgct ctatgccaag
     9061 aacctgctgc cgaaaacggt cgtcagccgg ccgatccagg gcgcgcctcc gatggtcgac
     9121 ctcgtcatcg gctacaacga ggccaacaca tcgccgctgc tgaaatttct gctgtccagg
     9181 ctggatgaat tgaagtttcg cgtgtcaagg cacgacgggc aatagatcgc ggctgcttcg
     9241 ccgcgtcgcc cgcgcagcgt tcagatggtc gccgcgtctg cgcgaaatct gcacaagaat
     9301 ccgcaaattc tgggcatgcc gcacataggc gctgccaatc ggtggggtat cgtcgggcca
     9361 tggggaaaac cacatcagga acataagacc atgtcgatcg cgaccgcgtc cgatttcgaa
     9421 attgctccca cgcgcccgct gttgagggcg ctgatatccg ccaccgcgtg cgtcgcgctg
     9481 gcggactggc tattttatgg ctggcagatc ggcatttcgc tggcgctatt tttcggcgtg
     9541 ctcggcgtgg tcgccgttgc cagcaatcgc gggcgcgcga caggaaataa tcaaatcgcg
     9601 atgtccgcgc tgttcgtcgc cggcctgctg ccgctgatcg aggacgtcaa catcctgtcc
     9661 gtcatcgtga gcacgctggc aaccgcgctg ttcgttatcg tcatcacgac gcgcgaaacc
     9721 ttgtcatggc agcggaactt gttcgaagcc gtggtcacac cctttcgcgg cccgtttcaa
     9781 ctggccggcg acttgttcgg cgcgctgcgg ctgatgaaac ggcggactcc gcaatggctg
     9841 agcctgagct ccctgatcgg ctggatcgtt ccgttgagcg ttttcttgat cttcctggcg
     9901 ctgttctcgt cgggaaatcc gctgatcgaa tatcggctga tgcagatcga ccttcgcgtt
     9961 ctcttcgacg ttctcgacct ccagcggatg gcgttctggc ttctggtcct ttgcgcgatc
    10021 tggcccttga tcctgcggcg catccgacgc actccaaggc aggagcccga atcctccagc
    10081 accgtcgcaa ccgaaccttc cgatttcgat tatctgtttg gcgtgcaggc catgctgcgc
    10141 tcgctggttc ttttcaacgc gctgttcgcg ctgcagaccg ggctcgacct tgtctatctc
    10201 tggggtggcg cgaccctgcc cgacggcctg agccatgcgg aatatgcgca tcgcggcgcc
    10261 tatccgctga tcgtgacggc gctgctcgcc gccggctttg tcctcatcgc catgcggccg
    10321 ggcggacccg tggaaaaatc cagactgatc cgtccgctgg tcctggcgtg gaccggacaa
    10381 aacatcctgc ttgtggtctc ctcgatcctg cgactcgatc tctatgtcgc cgcctactcg
    10441 ctgacctatc tgcggctcgc ggcattgatc tggatgggct tggtggccgc gggactgctg
    10501 ctgatgctga tccagatcgc atcgcaaaag cctaattcct ggctggtcac cgccaacgcc
    10561 gccacactgg cccttgtcct gtacggatgc tgtttcctca acgcgccatg ggtggtggcg
    10621 tcctacaatg tcgatcattg ccgggaagtc ggcgggagcg gacccaacct cgatctgaaa
    10681 tatctcttct cgcttgggcc tcaagtcctg ccggcccttg aacctcgtct gcagcaaatc
    10741 ccgggactcc ggccctacgt tgagggattt cggtttgatc aggatatcag tgcactccgg
    10801 atacgtcctg taaactggcg agcatggggc ttccgggcat ggcgtctgga acggtatttg
    10861 agtaataatt cctacacgtc gtcatatctt tcgaaaagcg acaaaggcta gtgaacgtgg
    10921 ctcattccat tctcgttgtt gatgatgacc cgcatatccg tgacgtcgtg cgcttcgcgt
    10981 tcgaaaaagc gggcatgctg atttccaccg cccaggacgg caaggaggcg ctgcggcagt
    11041 tcgaccgcaa cgtccacgag cttgtggtgc tcgacatcgg catgcccgag atggatgggc
    11101 tggaggtttg ccgccagatc cggaaaacct ccgatacgcc gatcctgttt ctgtcggccc
    11161 gcgacgagga gatcgatcgc attctcggcc tcgaaatcgg cggcgacgat tacgtgacaa
    11221 aaccgttcag cccgcgcgag ctggtggcac gggtgaacgc gatcctgcgg cgggcgcgga
    11281 ccacggccgc gccggccgtg ccgaaggcca tgaaccatgg cgggctcacg atcgatccgg
    11341 acgggcgcag cgttgccttc cacgacaagc ggatttcgct gacggcgctc gaattcgaca
    11401 ttttgaaaac gatgctggca cggccgggat tcgtgttcac gcgcgagctg atcctcgacg
    11461 ccgcctatgc cggcaatatc catgtcgccg atcgcaccat cgacagccat gtcaggaaca
    11521 tccgcgccaa gatggccgct gcaggctgcg agtcggccat cgaaaccgtg cacggcgtcg
    11581 gcttcagact gggacgctgc gaggccttgc gttgaccgac gccttggcca acaccagccg
    11641 cagatggcgg ccgagtctca gcctcgtcgt ttttctcgtc ctgaccagcg tgctttcgct
    11701 gccgctgttc agcctgtatt ttctcaaggt ctaccagaac gagctgatcc agcagaccga
    11761 agccgaactg atcgcgcaga gcgcggcgct gtcggcgatt ttccatcgcg aagtcgaaac
    11821 cagtattccc caaggtgtcg cgctcggcac caaggtttcg cccgtcgctc gaacggcctc
    11881 cgaagagccc tatcaaccga tctggccggc gctggagctt gccaacgaaa gcgtgctgcc
    11941 gccgaggccg agagctcgca caccgtccgc gccggccgat cccgctttcg cagcactcgg
    12001 tgcgcgcatg acgcccgatc tcgtcgcaac ccaaaatgtg acgctcgccg ggtttcggct
    12061 gctcgaccag aacggtgtcg tcatcgcggg gcgcgaggaa gtcgggatgt cgctcgcgga
    12121 tctcgaggaa gtcagcgagg cgctgcaagg gcgcttcagc ggcgtcttgc gcgtgcgcat
    12181 ctcaaagcat gaccagccgt cgctctattc gatcagccgg ggcaccggca tgcgcatctt
    12241 tactgcgatg cccgtgatcg tacgcgatca ggtcgcaggc gtcgtttatg cctcgcgcac
    12301 gcccagcaac gtcttcaaat atttgtatga gcggcgcaga agactgatgc tcgcgatgct
    12361 ttccatgatc gttccgaccc tgctgatcgg attcttgttt catcgcacca tcaccgagcc
    12421 gatgcgcgag ctggtcgaac gaaccaacct ggtgggcaaa ggcgaccgcg atgcgctgcg
    12481 cccgctcaag cgtcacggca ccagcgaatt cgcccgcctg tcgcagagtt ttctggagat
    12541 ggcaagaagg ctgaatgcgc gctccagctt catctcgacc ttcgccaccc acgtatcgca
    12601 tgaactgaaa tcgccgctga catctattca gggggccgcg gagcttttgc gcgacgatat
    12661 cgatgcggcg acgacaccct tgatgtccga cagggacaag cgaaaatttc tcgacaacat
    12721 cattgccgac gccgaccggc tggcgaaaat ctcgggccgc ctgcgcgatt tcgcgcgcgc
    12781 cgaaaatccg gttgcggtcg gcgccgcaag actgtcgggc cttatggctg gcttgcgttt
    12841 ggccttcgcg tcgctcgaca tccgaacggg cggcgacctc gacaccccga tgcggatatc
    12901 ggaggagaac gccggcatca ttttttccaa tctcgccgac aacgccctgc gccatggcag
    12961 ctcgacgctc gatatctccg cgacgcggca aggaaatctg ctgcaggtga tggtcgtcga
    13021 caacggcgaa ggcgtctcgc ccaacaaccg gacccagata ttcgacagct tttttaccac
    13081 ccggcgcgac agcggcggca ccggcatggg gctcgccatc gttcgcgcca tgctcgacgc
    13141 ccatggcggc gcggtccggc ttggcgactg cgaacgggga acggcgttcg aactgacaat
    13201 tcctgtcgca gatgaggcac tggtttcatg aacacagcgt cagaccacct gacgcgagag
    13261 cgcactccgg gcgccccaat gaccatgcgc gccggcctgt gcctgctgat cattgtctgc
    13321 ggtctcgcct tgcgcaggtt cggaccgggc ctcggcctac ccttcttttt cgtaaaatat
    13381 ggcgggtccg tgctgtgggg aacgatggtg ttctttctca tcgcgatggc cgcccccgcc
    13441 cgctccccgt cggccatcgc actgatatca gccacggtcg ccatctgcgt cgagctgttt
    13501 cggctggtcc ataccccatg gctcgacgat ttccgactga cgactttggg cgcgctgctg
    13561 atcggccgcg tgttctcgcc attgaacatg ctcgcctatg ccgccggtat cgtttcggga
    13621 atgtggctcg atcgcctcgg gatatcggcc ttcgcgagcg caaggctctc ggcgcgggac
    13681 gcccctcact ccgcgagctg aaaccgctcg aaccggtgca gctcttcctc gattcggcgc
    13741 ttcaggtcct tgcgcgcccc ccgcggcgcg gcgtcaaccc agctccattt ctgcatcagc
    13801 aatttgcggc tcttgcggtc ggtcttcaga tcgatggcgg caacgatgtt ttcgcccacc
    13861 aggaccggca acgcgaaata gccgaacacc cgtttttccc tgggcacata ggcttcgaag
    13921 cgatggccgt agccgaaaaa cagttcggtg cgcttgcgct gaatgatgag cggatcgaac
    13981 ggcgagagga tgtgcaccgg ttcgaaagcg ccgccgtccg ggatctccag cgtttcgggc
    14041 cgtgcccaat gttcctgctt gcccgcgccc tccagcgcga ccggcaccag ttcgttgcga
    14101 cggactctgg tctggatcaa acgccggatc gaaggcttgc tcggcgcatc gagatggcag
    14161 atcgaatcca gactcacgac gccttgcgcg cgcaacgcgc gatcgagcag ataggcggtg
    14221 gtttcgcccg ccgaggcagg ttttggcggc ctgtcccagc cgaaatgccg cgccatcagg
    14281 tcgtaagtct tgagcatgcc gttgcgctcg ctgatcgtca gcacgccggt atagaacgcc
    14341 agttgcagcg cccgcttcga cggcttgcgg ctggcccaca gatgctcctt ttccgtcagc
    14401 acatcgtcgt cgatgtcgcg gatggtcagc gcgccgtcgc gccggatcag ccgcagcacc
    14461 ttgcgcaaat cggccggctt gaccgagctg agccatttat ggccctcgcg cctgtgacgt
    14521 ttcatggcgg gaaggaagaa gcgcagatcc ctggccggca cgtaggacag cgcgtgggtc
    14581 cagtattcga acacgcttct gtcgacgctc tgggcctggt gcagatccgc gcgccggtag
    14641 ttcgggatgc gggtccacag aatgtgatgg tggctgcgct cgatgacgtt gatggtgtcg
    14701 atctggacat agccgagatg ttccaccgcc gcggccgtcg cctgcggccc ttccccgaac
    14761 ggcgctgacg tctcgagtcg ttgggcgcgc agccagatcc gccgggcttg cgtcttggtc
    14821 agggggtggg attgaatggt gcggggcatg gcgccgcaat gtagctggat tcgctgccgt
    14881 gagggtcagc ggattcccaa aaatgcgtca tcatcatatt tgtcttggcc atgttgtcgc
    14941 ccgtgcaggc catcatttca tcggaacctc tttggaaatg aaaccagcgt attgctggct
    15001 atcgatatac gttttggctt cgggcgtgtt tcttcaacca agacactttt gtgtactgcg
    15061 aagcagtgga aaagactccg tcaggccgtt ttttcgccaa gaccaccgtt aatgaaccgg
    15121 gaacccggtg ccgcacaatg agctgatctg cgccggaaaa atggagacgt aaatgcgaaa
    15181 gattttgatt cccttcgtag cagctgcctt ggctttctct ggcgcggcct ttgcccaaac
    15241 cggaggcggc accagcgggg gtgccgcggc taaatcggga accgacgcga ccgcgccggc
    15301 ggccggtacc gccaaagagg gcatggcgcc caccgagaag aaaatgaaga agaaaaagaa
    15361 gatgtaatcg gcaagattgc acgcagacct caccggcagc aacctgccgc caacaagccc
    15421 cggtaccaac cggggcttgt ttcgcaactt caaccggacg cagaacgcca gccggtatcc
    15481 gaatcccgat caacgtttcg gacgctcccc gatggccccg gtcgcgatct cagcctcgca
    15541 ggatgccttg ccgcgttccg gggcgcggca tttgtaaacg ctgccggtga gccgatcgat
    15601 cagccacacg ttgtcctcgc tgggattctc cattcccacg aaccgcgtgc ccaccgccgt
    15661 aatcagcgtg gacagcaata ttgccacagc gataatgatt gcgccgatca cgatgggcat
    15721 cgaggttccc aagggcgcgc gatccggcgt ggccgcccga tatccctgat attcggttgg
    15781 tcttttcggt gactgaaaca aggccacgct ggttttgctt tttctttctt ttcgattttt
    15841 tcttggcgga cggtttgatt acgcgcctat ctggcccatt tgttgttcgc agatcggcgt
    15901 tccggcgacg gctttgagac cagggtcgcc gcgccaacga acgccgtgcc tgcaagccac
    15961 ggcaaaaaga ctgcccgcag gccgccgaac tgaagcagcc gctccggcat cctgtcgccg
    16021 ctgcggtcgc gccttccacc aagccgacat tgcgcaggag ttcggccgcc gacctcgcgt
    16081 cgatcgccgg gttggaaagc ccaacgacgt tcttgtccgc gcccttgccg tcggaatctg
    16141 ctctctcccg tcccttttca tcaaggctca gcctggcgtg acccccatca cattcgcttt
    16201 cccccgcccg tcctaccgtc tgaccaccaa aaacgcttca gatctcacaa tgaggatacg
    16261 acaatgacct ttcacagact ttccgccctc aaggcgattg cgctcggtgc ggcgctgtcg
    16321 atgacagcgg gccttgccat cgccgccgac agcaatatct cggcggatca gatcgtcact
    16381 gcgctgcgcg ccaagccgct gacccgcggc ctgtcgaccg gaccgcaatc cgatgccgcc
    16441 gccactgcca gggtcaggga aatcggcttc gtgcagacgc tgcgcaaccg gaagacccgg
    16501 tcgctctcga tgaacgagcg cgaggaaatc gcggagatcg catcgaccaa gccgaatatc
    16561 gatctcgata ttcagttcga ctacaattcg gccgacatca gcaagacctc cctgcctgcg
    16621 gcgagggaat tgggcaaggc gctctcgaat ccggatctca agggttcgac attcgtagtc
    16681 gccggccaca ccgatgcgat cggcggcgaa gaatataacc agggcctttc cgagcgccgc
    16741 gctgaaacga tcaagcgcta cctcaccgag aagtatggca ttgccggcgc cgacctcgtc
    16801 accgtcggct atggcaagag caagccaaag gatccgaacg cgccgctgga cccgatcaac
    16861 cgccgtgtcc aggtggtcaa catggccagc aagaccgcgt cgaactgacg gttgctgcgc
    16921 ttgaccgcct gctgctctgc ggcaggcggt cagaatgcat gttcggtccg gccggccgtc
    16981 agattggatg gccggcctgt caaaccgatc ttcaaatacc tgatgcaagc ctttgatcga
    17041 acgcattttc ccgcgcgcgg gggatccgcg actgcacgaa caccatctgg ttgaagaagg
    17101 cgatcaacgc agatgcgacc gcggggcctt gcgcggtgaa cagcgcgagg ccacacaggt
    17161 aaatcgccac aatcgcgagc gaaccgaaca gcacgctcgg atagcgccag cccagcggca
    17221 catccccgaa cacccgaata gataccgcca tcaactcttt cgccagcggc ggatgcatcg
    17281 ggttgagcag cgggcttggc aagaccggct cgagcatctg ccgtgcggcc ggcacataat
    17341 gcacttcatc gaaatagaat ttttccggcg tcgtcacgcc gaccagaagt gcgaggtgtg
    17401 caacgactag gaccatagcg acaaaaatga cgctccgcgc agtggacttg tcaaaaacag
    17461 gcggtaattt tcgcacgggg ctgacaaatc gatggcgttg caacgattgc gtgtcattcg
    17521 aatggaggag agacggatct tttacgtcca atgcattgaa cgtatttcgc tttcgcgtgc
    17581 accaaccaat gggatggtta tgggccgctt gtggcaaatt tacaatatcg gccgcctgcg
    17641 cggtccggta ccgcgggcga ataatcgatc ggtattgaat tcggtatttg aaatgaattc
    17701 gcgtcacggc gttctctata ttttcgcgtt gtgcatggca gcagcccttg cggcgagtcc
    17761 cgctcgcgcg cagacgcgcg tcggcgaagc ggctgtcgtc aagaacgaag tcgtccgcgt
    17821 gatggcgtcg gcaaccagcc agatcaacgt tggcgacggc gtgctgcgcg acgagatcgt
    17881 gcgcaccggc ctcgacagcg cggcgcggct ggtgatggcc gacagcacca atctgtcgct
    17941 cggccccggc gcgacggtca aactcgaccg caccgttttc aacgaagagc acacctatcg
    18001 cgatatcgcg atcaggttga cgaccggcgc gtttcgcttc gtcaccggac actcggaaaa
    18061 ggccgcctac aagatcacca cgtcggtcgc gacaatcggc gtgcgcggca ccatcctcga
    18121 tattttgtcg caacggggca agaccaccgt ggtgctgcag gaaggcgcat cgcatgtctg
    18181 caccatcggc ggccagtgca ccgagctgac ccagcccggc gataccgccg tcatcacctc
    18241 ggccgccggc agggtcacga ttcagaggac caacaacccg ccctggacct tcgccgggac
    18301 ctgcggagcg gcggcaggac tttgcagcac cacgcagttt gcggatgcaa cgccatccgt
    18361 gacgcctccc gtcgatgacg gcagcgatcc caccggcatg ctgtgcgggc gctgacgatg
    18421 aacaaattca cgcgcagtct gatcggttcg gccacgctca ccgcgttgat ttggctggtg
    18481 ctggtacaga tccagccatc acccgcctca gccggggacg aatgtttcga ggattgtccc
    18541 gtgaccgtgg tggtggcgac gccacccccg ccaccgccgg taaccggcgc agattccagc
    18601 gccggatcga tcggcgacct gggcaggcag cgcttcaacc agatgatcat caaccgcgtg
    18661 ctgggctcgg tgctgctcgg cgtcaatgag caggtcaatt gcagcgactg cgtcagcgcg
    18721 ttcggatcgg cgggatcatt ctcggccggc atccacggcc gcaaggagct tacccccaat
    18781 ctgtcgctgc tggcgggtat cgcctacacc caatacagcg aaggcggata ccacatcacc
    18841 agcgcgccga tcggcgcgtt cgcgctccgc tacgatttcg tcgactgggg gtcgtcacgc
    18901 ccgttcttcg acgtcggcgc catcctgtcg ccatccgaga aggcgcgcta tagccgcagc
    18961 tacatgacga gcctcggtcc ggtttcgctt gagagccaaa cccacagcga gaactacggc
    19021 gcctacggcc gggccggctg ggtcagccgc atctcgccgc gcgacgaggt cgccgcgtcc
    19081 gtcgaggtgt ggcagctctg gcagcgggtg aacggctaca gcgatccgac cgtcgcattc
    19141 aatccgttcg acgccacgat tgcaaccggc accgacaaga ccaatctcgt caaggtcggc
    19201 ggacagtgga cgcatctgtt cggcaacagc gtcgagggca acatcaatgg cggcttcgtg
    19261 cagtcgttcg cgaaccattc gggcatcgta gcctccgtga ccggcaacgg caccgtggtg
    19321 acgccgacgc tcggaaacca gagctggttc gaatatggcg gccggctcgg ctttcgcatc
    19381 accaagggct gggtcgccga cctcttcgtc aacggcaccg cgggcccgca gccggtcggc
    19441 aataccatcc atggcggcgt cggactgcgg gtgaactact gagccgtagc gccggtgccg
    19501 cgcgcggcat ttcgaacggc gccgtccaag aatgcttcca gggctacgat gcaatcgcga
    19561 tcgcgataga cgcgatgctt gttcgccgca atcctggcgg acaattcaac ccgtttcgtt
    19621 gcattgcggc cgagaacacc cgcaatcgaa acatagtctt cgattgtccg cgccgtcgtc
    19681 tcgctgatat ccatcatgtc caggatggcg gcggtgtggc gtccccgcat catcgctccg
    19741 gcgaacgata caatcggaag gttgtgaacc aggctctcca ggatcgagtt gcaacccgac
    19801 catccgatgc tgtccaggac gacgtcgcat tgaccgatcg ccgcgacgaa gcggtccggc
    19861 gcaagtctcg gcaggaagac gcaatggttt tcggcgtcga ggccgccggc tttgaacgcg
    19921 cgccggagcc ggcctcgaaa catctcggtg acggtcctgc cgccagcgaa ttcgataaag
    19981 gtgaactgac agtccggaac ctcggctgcg atgcgtgcga acacttcgtc aaattgcggc
    20041 agatattttg gcaaggactg acagcaccag tagacgacgg cgcccatgcg cagaccaagc
    20101 tgtgcgcggt cgatcgcaac cggcggaacg tcggccggct cataatagat cgaaaggttc
    20161 ggcagccgga tcagctgttc ggaatagtga tctgccgcgc tttcgggttc catgagattg
    20221 ctgctgataa aataatcgat cgtcggaaat ccgctggtga cgggatgtcc ccaggaagcg
    20281 cattgcaccg cggcgagccg ttgcgccgcc agttgagccg acacattgtc catgccgatt
    20341 tcgggaaaaa tcaggacatg aggcgcgtcc gccaatatcg cgcgccgcca ggcatcgagc
    20401 gatagcggac cctgcacgaa acgatcgcac attgtcgccg ccacgtcggt ttcgccatcg
    20461 cggtcggcgc tcgtgtagta gcctgagacc tgaaatcggg tccggtccag catcttgagc
    20521 catcccttga gcggaatctt ccaattcgag tgctgcctga aaaaaccgct gacgatgccc
    20581 agcctgatcg gttcgcgcgg gctcggaggg gccggcaagg cagccgtcgc gtatttcgca
    20641 gccatcactc cgcacacgag ccgcccatac agggcctgaa gctctcgatc gttacggccc
    20701 tgatagggca aataaaacgg ctgatgggag ccgatcgcct ccgagagaac ggcgggtgcg
    20761 ccggcgcttt ccgcatcggc gcagagcctg gccagccggc ccgcataggc ggcacgccgc
    20821 tcggcgacgt cagatgcgtc ctggtaaatc gccggaagct ccgccatgca cagggccagt
    20881 tcggcttgcc agaaatccgg cttgagcgcg aacgcctggg cgaaatgggc tctcgcctcc
    20941 gcgatccggg tctcgccgcg gagcgctacg ccgcaattgt aatgagccat gaaggagccc
    21001 ggatcgatcc ggagcgccgt ctgatattgc gcgatcgcct catcgcgccg tccaagcgcg
    21061 cggagaatat tgccaagatt gttgcgcacg ccggcactgt ccggacgcag cgcgaccgcg
    21121 cgctccgcat gcgcaagcgc ttcaggcaga cgatccgcat tctgaagcag ggaagccaga
    21181 tggatgtaag catcgacaaa atcggggcgc agcgagatcg caagccgata atgcttctcg
    21241 gcgttcggat gttggccggc cagttcgaac gccgaggcca gattgaaatg cgcgacaggg
    21301 gattccggtg ctcttttcag gaccagttca aactgcgcca ccgcttcgtc aaggcgtccg
    21361 agcgatcgta atgcccgtcc gagattggtg cgagcctcgc catatccagg gttaagcgtc
    21421 acggcccgct cgaagcagga tatggcatcc gccagcaatc cgcctgctgc gagaatcacg
    21481 cctctgtcgt tgtgcgcctc ggcaaaatcc ggatcgagcg ccacggcccg gccgagatac
    21541 gacgctgcat cggggcgtct caattgatgg gcgaccacgc ccaaaaggcg aaacgcccgg
    21601 gcgttgttgg gatcggtatc acaaacgatc cggtagaggc gttcagcgtc ggtgaggcga
    21661 ccggcgcgat gatgagccat cgccgctgca agcgtctgtt cggagcggcc cggcgcgctc
    21721 atggcgatat ctcgaaccgg aatgatctca cgtcagtcgc aagaccgata gcgcccgaga
    21781 ccgaggtcga cctcgcaggg gctgccatag acccagaccg gctgatcgca cggcagcgcg
    21841 cgcgtcgccc ggtcccgtgt catacatgcg atgttcaaag cgggatgaag aaccagccgg
    21901 ccgcctccat agggagccgg caggaccggt tgttcctgat agcggaccat gtagccgtgg
    21961 tgatgatgcc ttgtcggacg tcttggcgcc ttatcggcat tggcccatcc agggctgccg
    22021 ccgagggtga gaatggcggc gagcgtgaca atggttcgca tcagcatgat ccctgtcctt
    22081 tattctaagc gccttcgatc gagaatgcgg ccggcgtctg gcaggcaagc caagtctgcc
    22141 acatccgcgc ataggccgcc tcgatatttg cggtcaattt tggcagatca aataatgcac
    22201 tggcgtcgcg attgttctgc agcttctgcc gcagcgcgat caggcgttgc gggctccgcg
    22261 ccagcgccag cgccgcctgt tcatattctt caagcgattt tgtcaccagt tccggcatgc
    22321 cgatcgccat c
//
LOCUS       DATNOI010000408         3895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2027927,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000408 DATNOI010000000
VERSION     DATNOI010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2027927"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VN968_16345"
     CDS             <1..795
                     /locus_tag="VN968_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL30840.1"
                     /translation="LVAIGTISMTLYVIHARRTASPVLDFALLRLPTMRASIIGGFLF
                     RLGIGSLPFLLPLLMQVGFGLSPLRSGLVTFASAVGAMGMKTLAARIIRTFGFRNIMT
                     INAVVSSIFLAACALFTIATPLLLIMILLVVGGFFRSLQFTAINTLAYAEVEPAQMSR
                     ATTLVSVNQQLAVSAGVAVGAFSVESTMLLHHVSELSAADFAPAFIVVSVISAVSAYF
                     FYEMPADAGHQVSGRGVLVVASPERDAEPEETAAAETANARDQRLG"
     gene            complement(962..1660)
                     /locus_tag="VN968_16350"
     CDS             complement(962..1660)
                     /locus_tag="VN968_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HXL30841.1"
                     /translation="MAKDTTGRLRVTVKSGGRLKLSSKLWLERQLNDPYVAQAKRDGL
                     RSRAAYKLIEIDDKYHFLKRGITVVDLGAAPGGWSQIAAKRVGAADGKGKVIAIDLLE
                     MPEIAGVSFAQLDFLKEDAPAKLLAMMGDRADVVLSDMAANTTGHRKTDQLRILGLVE
                     GAAAFASDVLNPGGTFVAKVFQSGADASLMTQLKRDFATVKHVKPASSRKDSSERYVL
                     ATGFRGVQKDRPET"
     gene            complement(1717..2787)
                     /locus_tag="VN968_16355"
     CDS             complement(1717..2787)
                     /locus_tag="VN968_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HXL30842.1"
                     /translation="MNDDTRLRDGLGPRASPPGSAAAGGGAQAELASGSGVYAALDLG
                     TNNCRLLIACPTGDGFRVVDSFSRIIRLGEGISATGSISEAAIERAITALSICRDKIH
                     SKKARRLRLIATEACRAASNAEGFRDRVAAATGIRLEVIDRETEAALAVIGCSPLLDQ
                     KGRGAILFDIGGGSTELVRVERDPEEQNPTPRIKAWMSIPLGVVTLAEHFGGRNVTKE
                     SYALMVQEVAQHIAPFAAEHGADLADMHLLGTSGTVTTLAGVHLNLARYERRRIDGVW
                     MSDADVTATIARLLGMSYQERAYNNCISVERADLVLAGCAILDAIRRAFPLPRLRVAD
                     RGLREGMLVEMMREDGLLSGSR"
     gene            2981..>3895
                     /locus_tag="VN968_16360"
     CDS             2981..>3895
                     /locus_tag="VN968_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL30843.1"
                     /translation="MQDRPSSASLETAIALQKFGVGQPVRRKEDDTLVRGKGKYTDDF
                     NLAGQAYAWIVRSSHAHGIIRGIDTSAAKAMPGVLGVWTGVDLAAANYGPYTCGLPLK
                     SRDGTPLLQTNRTALMTDKVRYVGDPVAFVVAQTLVQARDAGEAVVLDIDPLPAVTSA
                     EEAARPGAPQLYDHIPNNVALDYHYGDAAKVEAAFAGAAHVTKLDIVNTRVAVVAMEP
                     RAALASYDKASERYTIQVPTQGVAGNRANLAKNLLRVPNEKVHLLTANVGGSFGMKNI
                     NYPEYICILHAAKALGRPVKWTDERSTSF"
BASE COUNT          670 a         1276 c         1213 g          736 t
ORIGIN      
        1 ctggtcgcga tcggcaccat ttccatgacg ctctatgtca tccatgccag gcgaactgct
       61 tccccggtgc tggatttcgc gctgctgcgg ctgccgacga tgcgcgccag cattatcggc
      121 ggctttctgt tccgcctcgg catcggctcg ctgccgtttc tgctgccgct attgatgcag
      181 gtcggatttg gcctgtcgcc gctccgatcc gggctcgtga cctttgcgtc cgcggtcggc
      241 gcgatgggca tgaagacgct ggcggcgcgt atcatccgca ccttcggttt ccgcaacatc
      301 atgaccatca acgcggtggt gagttcgatt ttcctcgcgg cctgcgcgct gttcaccatc
      361 gcaacgccgt tgctgctgat catgatcctg cttgtggtcg gcggcttctt ccgttcgctg
      421 cagttcaccg ccatcaacac gctggcatat gccgaagtcg agccggcgca gatgagccgc
      481 gccaccacgc tggtcagcgt caaccagcaa ctggcggttt ccgccggcgt cgccgtcggc
      541 gcgttctcgg tggaatcgac catgctgctg caccatgtca gcgagctgag cgccgccgat
      601 ttcgcgccgg cgttcatcgt ggtgtcggtg atctcggcgg tttcggcgta tttcttctac
      661 gagatgcccg ccgatgccgg tcatcaggta tcggggcgcg gcgttctcgt ggtcgccagc
      721 ccggagcgcg acgccgaacc ggaggagacc gccgctgccg agaccgccaa cgccagggat
      781 cagcggctgg ggtaggtgta agcgataact tgcaacatcg tcatgcccgg ccatagcagt
      841 ctggcttgcg cagactgcgt aaacttgtct gcgacgccgg gcatccacgt ctttcttcgt
      901 gtgcccaaag caagacgtgg atggccggga cgagcccggc catgacgagg cgagagcagc
      961 tttatgtttc cggtcgatcc ttctgcacgc cccgaaatcc cgttgccagc acgtagcgct
     1021 cggacgaatc ctttcggctc gaggccggct tgacgtgttt gacggtcgca aaatcgcgct
     1081 tgagctgtgt catcaagctc gcgtcggcgc cgctctggaa caccttggcg acgaacgtcc
     1141 cgccgggatt gagcacgtcg gaggcaaatg cggcggcgcc ttccaccagg ccaaggatgc
     1201 gcagctggtc ggtcttgcga tggcctgtgg tgttggcggc catgtcggac agcaccacat
     1261 cggcgcggtc gcccatcatg gcgagcaatt ttgccggcgc gtcttccttg agaaagtcca
     1321 gctgcgcgaa gctgacgccg gctatttccg gcatctccag aagatcgatc gcgatgacct
     1381 tgcccttgcc atcggcggcg ccaacacgtt tggccgcgat ctggctccag ccgccgggcg
     1441 cggcaccgag atcgaccacg gtgatgccgc gcttgagaaa atgatacttg tcgtcgattt
     1501 cgatcagctt gtaggccgcg cgcgagcgca agccgtcccg cttggcctgc gccacatagg
     1561 gatcgttgag ctggcgttcc agccagagtt tcgacgacag cttgagccgg ccgccgctct
     1621 tgacggtgac ccgcagccgc ccggtggtat ctttcgccat atcggtcttt tgccttggaa
     1681 agctgtctcg ctctaacaca ccgcgggtcg gcgcgcctat cggctgccgc tgagcagccc
     1741 gtcctcgcgc atcatctcga ccagcatgcc ctcgcgcaaa ccgcgatcgg cgacgcgcaa
     1801 tcgcggcagc ggaaacgcgc ggcggatcgc atcgagaatg gcgcagccgg ccagcaccag
     1861 atcggcgcgc tcgacgctga tgcagttgtt gtaggcacgt tcctgatagc tcatgccgag
     1921 caggcgcgcg atggtcgcgg tgacatccgc gtcgctcatc cagacgccgt cgatccggcg
     1981 gcgctcgtat cgcgccagat tgagatggac ccccgccagc gttgtcacgg ttcccgaagt
     2041 cccaagcaaa tgcatgtccg ccagatcggc gccatgttcg gctgcaaacg gcgcgatgtg
     2101 ctgcgcgacc tcctgcacca tcagcgcata ggattccttc gtgacgttcc tgccgccgaa
     2161 atgctcggcc agcgtgacga cgccgagcgg gatcgacatc catgccttga tacgcggcgt
     2221 tgggttttgt tcttccggat cgcgctcgac ccgaaccagc tcggtcgagc cgccgccgat
     2281 gtcgaacaga atagcgcccc ggcctttttg atcgagcagc ggcgagcagc cgatgacggc
     2341 gagcgcggct tcggtctcgc gatcgatcac ttcaagccgg atgccggttg cggcggcaac
     2401 ccgatcccgg aagccctcgg cgttggatgc cgcgcggcag gcttcggttg cgatcagtcg
     2461 caggcgcctg gcttttttcg aatggatttt gtcgcggcag atgctgagcg cggtgatcgc
     2521 gcgttcgatg gcggcttcgc tgatagaccc ggtggccgag atgccttcgc cgagccggat
     2581 gatccgtgag aacgaatcga ccacacggaa cccgtcgccg gtcggacagg cgatcagcag
     2641 ccggcaattg ttggtgccga ggtcaagcgc agcatagacg ccggagcccg atgccagctc
     2701 cgcttgcgcg cccccgcccg ccgcagccga tcctggcggg cttgcgcgcg gcccaaggcc
     2761 gtcgcgaagc cgcgtgtcat cgttcatcaa aaatgtcttt ccgcggccat ccggccgacg
     2821 aggaatttcc gttcacggaa actttagcag cgccgccagg gcgcgcaaca ggcgccacat
     2881 gggaccatgc ccaatcaggc gttgtcgtcc ggatataggt acgttatctg ggtggtaagc
     2941 gccgaaatcg cgcaaaatcc ggcatttcag gtatccttca atgcaagacc gcccctcttc
     3001 agcgtctctg gaaaccgcta tcgcactgca aaaattcggt gtcgggcagc cggttcgccg
     3061 caaggaagac gacacgctgg tgcgtggcaa gggtaaatac accgacgatt tcaacttggc
     3121 cgggcaagcc tatgcctgga tcgtgcgctc cagccatgcc cacgggatca ttcgcggcat
     3181 cgacacctca gcggccaaag ccatgcccgg cgtactcggg gtctggaccg gcgtcgacct
     3241 cgctgcggcg aattatggcc cctacacctg cggcctgccg ctgaagagcc gcgatggcac
     3301 cccgctgctg cagaccaacc gcaccgcgct gatgaccgac aaggtacggt atgtcggcga
     3361 tcccgtggcg tttgtggtgg cccaaaccct cgtgcaggcg cgcgacgccg gcgaagccgt
     3421 ggtgctcgat atcgacccgc tgccggccgt caccagcgcc gaagaggcgg caagacccgg
     3481 cgcgcctcag ctctacgacc acatccccaa caatgtggcg ctggattatc actacggcga
     3541 cgccgccaag gtcgaggccg ctttcgccgg cgcggcacat gtcacaaaac tcgatatcgt
     3601 caacacccgc gtcgctgtag tggcgatgga gccgcgggcg gcgctggcct cctacgacaa
     3661 ggcgagcgag cgctacacga ttcaggttcc gacccagggg gtcgccggca atcgcgccaa
     3721 tctcgccaag aacctgctga gggtgccgaa cgaaaaggtt cacctgctga ccgccaatgt
     3781 cggcggctcg ttcggcatga agaacatcaa ctatcccgaa tatatctgca tcttgcatgc
     3841 ggcgaaggcg ctgggccgtc cggtgaaatg gaccgacgag cgctcgacca gcttc
//
LOCUS       DATNOI010000409        18835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1256141,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000409 DATNOI010000000
VERSION     DATNOI010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18835
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256141"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1794)
                     /locus_tag="VN968_16365"
     CDS             complement(82..1794)
                     /locus_tag="VN968_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL30844.1"
                     /translation="MGVDGAAGNKPDARASLAARFPRLEQTFPALTPQEIARMRRFGE
                     VRSHKDGERLFETGKPGPGMYVILSGHVAISQRDGFGHVTPVIDQGPGQFLAEIGQLS
                     GRPALVDGHAEGDVETLLIPPERLRALLVAEADLGERIMRALILRRVSLIQAGVGGPV
                     LIGPSDSAGVIRLQGFLTRNGIPHHLLDPERDHDAAEVIARYSPSPSDWPLVVATDGT
                     VLRNPGETELARALRMIGPTGNKIYDVAIVGCGPGGLATAVYAASEGLSVVLLDARSF
                     GGQAGASARIENYLGFPTGISGQALAGRAFTQAQKFGADVMIPMRVKALDCTRTDGAF
                     ALTLDGGDTLRSRAVVVASGARYRRPDIENLEKFEGRGVWYWASPIEARLCAEQAIVL
                     VGGGNSAGQAAVFLSGHARKVYMVIRGGGLGASMSRYLIERIEATPNIELVFNTEVVA
                     LEGSEDGSLERVRWRSRLSAEASTVDIRNLFLFAGAEPATGWLDGCGVTLDRGGFVVT
                     GAQSEQNQGRPVPLLETSVPGVFAVGDVRSGSVKRVGGAIGEGAQVVAALHGFLGDRA
                     KPAL"
     gene            2086..2532
                     /locus_tag="VN968_16370"
     CDS             2086..2532
                     /locus_tag="VN968_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXL30845.1"
                     /translation="MADIPALAPLAQRTLDGWHRFVGSPDRDLLASLLSEHIVFRSPF
                     VQSPIPGRDATLLVLTTVVQIFENFRYHRTFVAGSHDVALEFAANIGKWQLKGVDLIH
                     FNESGEMAEFEVMIRPIKALAALGEEMGNRIGPQLARLKQAAAASC"
     gene            2651..2725
                     /locus_tag="VN968_16375"
     tRNA            2651..2725
                     /locus_tag="VN968_16375"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2683..2685,aa:Thr,seq:ggt)
     gene            3189..3413
                     /locus_tag="VN968_16380"
     CDS             3189..3413
                     /locus_tag="VN968_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30846.1"
                     /translation="MNAVLSSLAAYLVAGVRPPTPLAKAIALVLAVKLMAIAGAGIFL
                     FSSSGRLVVDSNAVSRLIGPSVSAANEGEH"
     gene            3415..4980
                     /locus_tag="VN968_16385"
     CDS             3415..4980
                     /locus_tag="VN968_16385"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HXL30847.1"
                     /translation="MLDFDPVGLARLQFAVTALYHFLFVPLTLGLSLLLAIMESVYVM
                     TGREIWRDATKFWGKLFGINFAMGVATGITMEFQFGTNWAYYSHYVGDIFGAPLAIEG
                     LMAFFLESTFVGLFFFGWDRLSKLGHLAVTWLVAIGSNFSALWILIANGWMQHPTGAV
                     FNPETMRMELTSFWDLIFNSVGQAKFVHTVGAGYVTGSMFVVAISSYYLLSNRFQAFS
                     KRSIIVGSAFGLTAALCVVVLGDESGYTVSENQKMKLAVIEGMWETEPAPAGFNVFGI
                     PDTESREVKYALKIPWMLGIIATRSFNQPLPGINDLVAHAEERVRNGLIAYEALERIK
                     QNRGDSQARQQLREHSTDLGYALLLKRYVADPRTADAATIARAAGDTVPNVGPLFWSF
                     RIMVALGFYFIALFAVAFWNASHYNFSRRWLLHLMLWSLPLPWIAAELGWIVAEYGRQ
                     PWAIDGVLPTFLAVSSVSTAQVLFTLTGFVLFYSCLLVVDIMLMLRYIRMGPVGALGP
                     PEPAIRPAAVPAE"
     gene            4990..6141
                     /gene="cydB"
                     /locus_tag="VN968_16390"
     CDS             4990..6141
                     /gene="cydB"
                     /locus_tag="VN968_16390"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HXL30848.1"
                     /translation="MTIPVDYETLRLVWWLLLGVLLIGFAIMDGFDLGVAMLLPYVAR
                     RDIERRVAINAVGPFWDGNQVWLILGGGAVFAAWPPVYATSFSGFYIAMFLVLATLIL
                     RPVGFEFRNKVADTRWRTFWDYALFAGGLVPSVVFGVAFGNLLQGVPFRIDSDLRALY
                     EGSGLFELLNPFGLLCGLVSAAMLATHGAVYLTLKTDGRVQQRARDFVKIGAVLTVVL
                     FVLAGLWVWLRIEGYAITSTVIGDGPSNPLMKTVVRQPGQWLANYGAHPWMIVAPCLG
                     IFGPLLALLFTTAQRGGLAFIASALGILGIIATAGVSMFPFLMPSNVAPMASLTVWDA
                     SSSQLTLFVMLMATLIFLPVVLVYTSIVFSALRGVVTASQIEGNSPNLY"
     gene            6151..6258
                     /gene="cydX"
                     /locus_tag="VN968_16395"
     CDS             6151..6258
                     /gene="cydX"
                     /locus_tag="VN968_16395"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HXL30849.1"
                     /translation="MWYFTWILGVAFACAFGILNAVWHEHHLPDETIDA"
     gene            6810..6992
                     /locus_tag="VN968_16400"
     CDS             6810..6992
                     /locus_tag="VN968_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30850.1"
                     /translation="MLSSDASNFSDADRRFVRAMIALGAPNNATAGAFGISEVELLAK
                     FPDELQSRPRGTARAD"
     gene            7175..7885
                     /locus_tag="VN968_16405"
     CDS             7175..7885
                     /locus_tag="VN968_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HXL30851.1"
                     /translation="MKKLLLGTVALVALGATVPALAADLAARPYTKAPAYAQPIYNWT
                     GFYIGGHLGGAFGGDNSLTGSDGRFLGGLQGGADYQFAPNWVVGIEAQYSWLDNSNNG
                     VLFPGGVLVTSNSDQLGSVTGRLGYTWGPALLYAKGGYGWKDGNNLNVSVGGVPQAFT
                     TNGNHRDGYTVGAGLEYMFAPNWSAKAEYQYYNFGDTTFTTGPAAIVGVRFRDDEHTV
                     KAGLNYRFNWGRPAVAGY"
     gene            8046..10193
                     /locus_tag="VN968_16410"
     CDS             8046..10193
                     /locus_tag="VN968_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL30852.1"
                     /translation="MAGLFTIGKPGWRSRFLALQWGVRASLFAAFAVIAGMGLLISAG
                     AGMVLGHLGERMVDLSARDIPRLAASLQLSAQSASLASQGPALLASRSEEALNERAKK
                     MKETQAVTFDKLGEVIELGADRAVVAALTETVRNIDETIKSLVAAARERLEVVAQHEK
                     QYDALRRAQAEFVAAANPAMLDAQIQINAILGSAMLSQDDAIQVARTIDQLGNAVASG
                     NLMASEMTAALSASSSATLEAIEKDFKSAQARLKSNLDLLPQNSTTRSLKDAALKLLA
                     LGEGKSGVFKLRQKELDANDYGQLVLDETRKLNVGLGISVQQLVSGVQSETDGSTWQA
                     RKEISLATTVMLALGALTLVGSVLFVWLYVGRSVLRRIGHLQRSMQLLSDGDLESEIY
                     RSTQRDEIASMANSLQIFRESMIEARALSADQDRDRIGKTERASRMETRIAEFEATVR
                     TALDGLQKSANSMQATAQSMSATADQSSALVNAVASAAEETSVNVQTVSAGTEELSSS
                     IAEIGRQVVTSAQIARKAVEEAGATDATMQGLADNASRINVVVDLIQVIASQTNLLAL
                     NATIEAARAGEAGRGFAVVASEVKSLANQTAKATDEIRAQIASMQQVTTSAVGAIRNI
                     GQTIGEINDVTTAIAAAVEEQGAATREIARNVQHAAGGTAEVSSNIVGVSTASAEAGS
                     AAGEVLSASSALRREADVLRAEIDAFLSNIRAA"
     gene            10424..11485
                     /locus_tag="VN968_16415"
     CDS             10424..11485
                     /locus_tag="VN968_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL30853.1"
                     /translation="MHSRTDTVHSSAEALRYPWENHPGPDQAVEVMPGVLWVRLKLPF
                     RLNHVNIYLLADGEGWTMVDSGFGNEESIAAWTALFEGPLAHVKITRLIVTHSHPDHV
                     GLAGWIVERFSCPLHMSQVEYLQSVYHQNRGTEERRNAQRLFFRRHGMDESLTDKLLG
                     RGQDYLKRVSILPPSYRRISHGDEISIGRRRFKVITGGGHALDQVMLYCAADKLFLSA
                     DQVLSKISPNVSVWAVEPDQNSLGEYLASLASLTTTLPYDVIVLPGHGVPFYGLKTRI
                     KQLADHHEDRCRLIAEACQEVPKTSKELVPVVFHKHALDVHQMGFAAGELIAHVNYML
                     VEGRLTAEEADGVLRFRTT"
     gene            complement(11550..12635)
                     /locus_tag="VN968_16420"
     CDS             complement(11550..12635)
                     /locus_tag="VN968_16420"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXL30854.1"
                     /translation="MHPFNDAEVARRLLDHLAHAFGCPDAVYLAGPTRIQGGFDAAIF
                     GFTLDRVTPPLVGPLILRLSHANADPARVKLETIVQNTLAEMGFPAPRVIVTESDQDL
                     LGGPFMVMTRLSGQTLAHDIEGLGAGTSLVGQLQLLFNLPAILARIIDQWVDMQIRLH
                     QLPAETLLRAVTAAGIDAGAITFEGQLARLRAIVGRCPLTGLQPGLAWLDDHRPSKAR
                     DAAICHGDFHPLNILADNNEPTGVIDWANAVIAEPAMDVGSAIANMSAVPLPLPWALR
                     VTARAIIGAALRRYERTYRELRPLDDQAVLYYEVFRAVAQLVWVGQARAAGRAGGGAF
                     HSGAGVGNLIALIRKLSGVSLWLEKRA"
     gene            12922..13353
                     /locus_tag="VN968_16425"
     CDS             12922..13353
                     /locus_tag="VN968_16425"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXL30855.1"
                     /translation="MPDVLNLISRIVLSAVFIVYGYLAFADVKAWVNFNQAALKRFFD
                     VVAGGMAPPTWFGYLIATIQLVGGLAILAGFKTRWVAYAFVAYLLFVEFFAHNFWDMQ
                     GGARGANMAHFYKNLAIIGGFLLLALQGAGRYSVDGQISRA"
     gene            13578..14807
                     /gene="hemA"
                     /locus_tag="VN968_16430"
     CDS             13578..14807
                     /gene="hemA"
                     /locus_tag="VN968_16430"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HXL30856.1"
                     /translation="MDYNRFFTTALNRLHDERRYRVFADLERIAGRFPHAVWHSPKGP
                     RNVVMWCSNDYLGMGQHPKVVGAMIETATRVGTGAGGTRNIAGNHHPLVQLEQELADL
                     HGKQAALLFTSGYVSNQTGIATIGKLIPNCLILSDALNHNSMIEGIRQSSCERQIFRH
                     SDMAHLEELLRAAGPDRPKLIACESLYSMDGDIAPLSKICDLAERYGAMTYVDEVHAV
                     GMYGPRGGGIAERDGVMLRIDILEGTLAKAFGCLGGYIAANSDIIDAVRSYAPGFIFT
                     TSLPPAICSAATAAIRHLKTSSWERDRHQDRAARVKAILTAAGLPVMSSDTHIVPLFV
                     GNPEKCKRACDILLEEHGIYIQPINYPTVAKGTERLRITPSPYHDDGLIDQLAEALLQ
                     VWERLGMPLHEKSLAAE"
     gene            14966..18475
                     /locus_tag="VN968_16435"
     CDS             14966..18475
                     /locus_tag="VN968_16435"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HXL30857.1"
                     /translation="MLHDWGVIAAAFGYIGFLFLVASYGDRMSPTQRGRAGTLIYPLS
                     LAIYCTSWTFFGSVGFASRTSVDFLAIYIGPILMIGLCTPLLRRVIQLAKSQNITSIA
                     DFIAARYGKSQAVAATVAVIAIIGSVPYIALQLKAVASSLETILSEDQAIASVPIIGD
                     MALIVTLAMAAFAVLFGTRQSDATEHQHGLMLAVATESIVKLVAFLAAGICVTFLLFG
                     PFELIERAMKTPEAVRAINYTPSAGNFLTMVLLSFCAIMLLPRQFHVSVVENSSDAEV
                     GRARWLFPLYLVAINLFVIPIAIAGLVTFPFGAVDSDMFVLALPIEGNAVLLSVGVFI
                     GGLSAATAMVIVECVALSIMVSNDIVVPLVLQRGPQSRAGERDFGDFLLRVRRFAIFA
                     IMVMAYFYYRALGNAQLASIGLLSFAAIAQLAPAFFGGLFWRRATARGAMAGMLVGVA
                     VWSYTLFIPSFLGGSPAGLLLLQHGLFGIEALRPQALFGADLPPLLHGVLWSLSLNLL
                     TYIVLSLAREPSSIERLQADLFVPDTLAPTTPTFRRWRTTVTVQDIQSTVAQYLGPDR
                     AHHAFEAFAVTHNTRLDPAAPADFELLQHAEHLIASSIGAASSRLVMSLMLRKRTVSA
                     KAALKLLDDSHAALHFNREILQTALNHVRQGIAVFDAELQLICSNRQFGEILGLPLHL
                     VQIGIPLQEILEFMGSISPAGLGDSHALLERRLAAYTTEGKPYLERLPDRHMVIEVRS
                     NRMPGGGLVITFSDVTPSFEAAEALERANATLEKRVRDRTEELTRLNSELALAKSTAE
                     DANISKTRFLAAASHDILQPLNAARLYVTSLVERQNGGEDSRLVENIDDSLEAIEEIL
                     GALLDISRLDAGAMTPSITSFKIGDLMRSLEIEFAPIARAKGLKLTFVPCSLPVASDR
                     LLLRRLMQNLISNAIKYTPRGRVLVGCRRHGQSLQLGVYDTGVGIPTLKRSEIFKEFH
                     RLEQGARIARGLGLGLSIVERLARVLNHGIALDSNVSGGSFFSVTVPIAKAINYTAAV
                     TSATSLSRTPMSGALVVCIENDPAILDGMRTLLTAWDAKVIAVADPEAAIGPIEAAGG
                     QVTGLLVDYHLDRGNGVAAIRDIRRRFGENIPAILITADRSPNVRAAAREENIAVLNK
                     PVKPASLRALLGQWRSQQMLAAE"
     gene            complement(18499..>18835)
                     /locus_tag="VN968_16440"
     CDS             complement(18499..>18835)
                     /locus_tag="VN968_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL30858.1"
                     /translation="ETLRDAIVKVMDGDVWIPPDTDLSAAADPDMARLRDRLVTLTPQ
                     QVRVLMMLSEGLLNKQIAYELGVSEATIKAHVSAILQKLGVESRTQAVIAAAKIAGGQ
                     WRQDAPPPE"
BASE COUNT         3366 a         5984 c         5764 g         3721 t
ORIGIN      
        1 ttcctcgcaa ccgagcgcgc tcggcgtgac gttgcggatg ccggcgatgt gtgtgcagcc
       61 ctttggcatt ccgatcatcc cctaaagcgc cggcttcgcc ctatcgccaa ggaaaccgtg
      121 cagcgcggcc accacctgtg cgccctcccc gatcgcgccg cccacgcgct tgaccgatcc
      181 cgagcgcaca tcgccgaccg cgaatacgcc cggcaccgag gtttccaaaa gcggcacagg
      241 gcgtccctgg ttttgttcgg actgcgcgcc ggtgaccacg aacccgccgc gatcgagcgt
      301 cacgccgcag ccgtcgagcc agccggtggc cggctcggca ccggcaaaca ggaacagatt
      361 gcggatgtcg acggtactcg cttcagcgga caagcggctg cgccagcgca cgcgctcgag
      421 agaaccatct tcgctgccct caagcgcgac cacctcggta ttgaacacca gttcgatatt
      481 cggcgtcgcc tcgatgcgct cgatcagata gcgcgacatg ctggcgccca ggccgccgcc
      541 gcggatcacc atgtaaacct tgcgggcgtg gcccgacaga aacaccgcgg cttgccccgc
      601 ggaattgccg ccgccgacca ggacgatcgc ctgctcggcg catagccgcg cctcgatcgg
      661 agaggcccag taccagacgc cgcggccctc gaacttttcc aggttttcga tatcgggccg
      721 ccggtagcgc gcgccgctcg cgaccaccac agcgcgcgag cgcaaggtgt cgccgccatc
      781 gagcgtcagc gcaaaggcgc cgtcggtgcg cgtgcaatcg agcgccttca cccgcatcgg
      841 gatcatgacg tcggcgccga acttctgcgc ctgggtgaag gcgcggccgg ccagcgcctg
      901 gcccgaaatt cccgtgggga agccgagata attttcgatt cgggcgctgg caccggcctg
      961 tccgccgaaa gagcgggcat ccaggaggac caccgagaga ccttccgacg ccgcgtacac
     1021 ggcggtggcc agcccgcccg gaccgcatcc gacgatcgcg acatcgtaga tcttgttgcc
     1081 ggtggggccg atcatgcgaa gcgcccgggc cagttcggtt tcgcccggat tgcgaagcac
     1141 ggtgccgtcg gtggcgacca ccagcggcca atccgaaggc gagggcgagt agcgcgcgat
     1201 cacttcggcg gcgtcgtgat ctctctccgg atcgagcaga tgatgcggaa tgccgttgcg
     1261 ggtcagaaag ccctgcaggc ggatgacgcc ggccgaatca gatggtccga tcaacaccgg
     1321 accgccgacg ccggcctgga tcaggctcac ccggcgaagg atcagcgcgc gcatgatgcg
     1381 ctcgccgaga tcggcttcgg caaccaacag cgcccgcagc cgctccggcg gaatcaacag
     1441 cgtttcaacg tcaccctcgg catggccatc gaccaatgcg gggcggcccg agagctggcc
     1501 gatttcggcg agaaactgac ccggtccctg atcgatcacc ggcgtgacgt gaccgaaccc
     1561 gtcgcgctgg ctgatcgcga catggccgga caggatgacg tacattcccg ggccaggctt
     1621 gccggtttcg aacagtcttt cgccgtcctt gtggcttcgc acttcgccga agcggcgcat
     1681 tcgcgcgatc tcttgcggcg tcagcgccgg gaatgtctgc tcgagacgcg gaaacctggc
     1741 cgccagcgat gctcgggcat cgggtttatt tccagccgcg ccgtccacac ccatctccgc
     1801 ctcgcatcgt caaaagctag caaaaacctg ccccgggtcg aacccggcta tttgcccggt
     1861 ttttttccgc cgacgtcata tggtgagacg tcatcaggat tgggaggtac cgtcgtgaca
     1921 tcgcctcggc cccgcgcctg cgattttcgc cgcttgagat tctcgcgcaa tgcgagcttc
     1981 agccgatcct gccgcggatt ttttccgcgc gctccgcttt gcccctcgcc tttatccttt
     2041 gcatccttca tcgtcgaccc gttcgaatga gctggagaga aaccgatggc ggatatacct
     2101 gcgctggcgc ctctggcgca acgaactctg gacggctggc atcgctttgt cggctcgccc
     2161 gatcgcgacc tgctggcgtc gctattgtcc gagcatattg tgtttcgctc gccattcgtg
     2221 caatcgccaa ttcccgggcg cgacgcgacc ttgctggtgc tgaccacggt ggtgcagatc
     2281 ttcgagaatt ttcgctatca ccgcaccttc gtcgccggct ctcacgatgt cgcgctggaa
     2341 tttgccgcca atatcggcaa atggcagctc aagggcgtcg atctcatcca cttcaacgaa
     2401 tcgggcgaaa tggccgaatt cgaggtcatg atccgcccca tcaaggcgct ggcggcgctc
     2461 ggtgaggaaa tggggaaccg gatcggcccg cagcttgccc ggctaaagca ggccgcggcc
     2521 gcttcctgct gatggcggcg gcttttgacg atgtccgagg ccggtcccgc gcttgcgcgc
     2581 gagggggcct tatggcaagg atcggccgcc ttgtagcggc ctttttggcc cagtccggta
     2641 tccggggcac gctgccgtag ctcagtggta gagcactcca ttggtaatgg agaggtcgac
     2701 agttcaatcc tgtctggcag caccagtcat cgtgctgata acgctgagat ttccttgtca
     2761 atttcgccgc gcgctcgtgc ccatattcga tggagccacc gcgtaaccgc gcgaacttat
     2821 cttgctaaac gagagccaca aatcctcaga taactggtcg catcaaccgg cccgggttag
     2881 catctgtcga gattccgatg gctcactaag cccgacccag ttcatttccc ttattgtgac
     2941 ggactcacag gcagtggcag acgtgtgacg tcgtatattc agcggcacct atatctccgc
     3001 tccgaccgag cttcgcgacc gattgctcgg ttgatttgcg ccgcccatcg tcgacttgat
     3061 ttgaatcaat ctcccgaccc gttttgttct ttgtgttcgt tgtgcggtac tgcaaaatag
     3121 cggctggagc gccgcatgac cgcgcggcaa tggagtgtcg tgaaaaggtt ttcttgcgag
     3181 ggcccagttt gaacgccgtc ctgtcatcgc tagccgctta cctcgtcgcg ggcgtgcgac
     3241 cgccaactcc gctcgccaag gcaatcgcgc tggtgctggc cgtcaagctg atggcaatcg
     3301 cgggtgccgg cattttctta ttttcgagca gcgggcggct ggtcgtcgac tcgaatgcgg
     3361 tctcgcggct gataggtccg tcggtgtcgg cggcgaatga gggggaacat tgacgtgctc
     3421 gattttgatc ccgtcggcct tgcgcgacta caattcgctg taacggcact ctatcatttc
     3481 ctgtttgtgc cgctgacgct tgggctgtcg ttgctgcttg ccatcatgga aagcgtgtac
     3541 gtgatgaccg ggcgggaaat ctggcgtgac gcgacgaagt tctgggggaa attgttcggc
     3601 atcaatttcg ccatgggggt tgccactggc ataaccatgg aatttcagtt cggaaccaat
     3661 tgggcctact actcgcacta cgtcggcgat atttttggcg cccctttggc gatcgaaggc
     3721 ctgatggcct tctttctcga gtcgactttc gttggtttgt tcttcttcgg ttgggatcgg
     3781 ctgtcgaagc tcggacatct ggcggtgacg tggctggttg cgatcggctc gaacttctcg
     3841 gccttatgga tcctgattgc caacgggtgg atgcagcatc cgacgggtgc cgtctttaac
     3901 cccgaaacca tgcggatgga gctgacgtcg ttctgggacc tgatcttcaa ctcggtcggc
     3961 caggccaaat tcgtgcatac ggtcggcgcc ggctacgtca ccggctcgat gttcgtggtt
     4021 gctatttcgt cctattacct gctctccaat cgatttcagg cgttctcgaa acggtcgatc
     4081 atcgtcggat cggcgttcgg ccttactgca gcgctgtgcg tggtcgttct cggcgacgaa
     4141 agcggttaca ccgtatccga gaaccagaaa atgaagctcg ccgtcattga gggcatgtgg
     4201 gaaaccgagc cggccccggc cggattcaac gttttcggca ttcccgacac ggagagccgc
     4261 gaggtcaaat acgccctcaa gataccctgg atgctcggca tcatcgcaac acgttcattc
     4321 aaccagccgc taccgggaat caatgacctg gttgcccacg ccgaagagag ggtccgcaac
     4381 gggctgatcg catatgaggc gctcgagagg atcaagcaga accgcggcga ctcccaggcg
     4441 aggcagcagc tccgcgagca ttccacagac ctgggatacg ccctcctgct gaagcgctat
     4501 gtcgcggatc cgcggactgc agatgccgca accattgcgc gcgccgcggg tgacaccgtt
     4561 cccaacgtcg ggccgctgtt ctggagcttc cgcatcatgg tggcgctagg cttttatttc
     4621 atcgcgctgt ttgcggtcgc attctggaac gcgtcacatt acaacttttc gcgccgatgg
     4681 cttttgcatc tgatgctttg gtcgctgccg cttccctgga tcgcggcgga gctaggctgg
     4741 atcgtcgccg aatacggccg acagccgtgg gccatcgacg gggtgctgcc gacatttctt
     4801 gccgtatctt cggtatcaac cgcgcaggtg ttgttcaccc tgaccggctt cgtcttgttc
     4861 tattcgtgcc tgctcgttgt cgatatcatg ctgatgctca gatacatccg gatgggaccg
     4921 gtcggcgcgc tgggaccgcc ggaaccagcg atacgacctg cagcggtgcc cgcggaatga
     4981 ggtggtgcaa tgacgatccc ggtcgactac gaaaccctta ggcttgtgtg gtggctcctt
     5041 ctgggcgtgt tgctgatcgg cttcgccatc atggatggtt tcgatctggg agttgcgatg
     5101 ctgttgccct acgtcgctcg tagagacatc gagcgacgcg ttgcgatcaa tgcggttgga
     5161 ccgttctggg acggcaatca ggtctggctg atcctcggtg gtggcgcggt gttcgcggca
     5221 tggccgccgg tctatgccac ctcgttctcg ggcttctaca tcgccatgtt tctcgttctt
     5281 gcgacgctca tcttgcgtcc ggtcgggttc gagttccgca acaaggttgc cgatacgcgc
     5341 tggcggacct tctgggacta cgccctgttc gcgggcgggc tggtgccgag tgtggtattc
     5401 ggcgtggcgt tcggcaattt gttgcagggc gttcctttcc gcatcgactc cgatctgcgg
     5461 gctctctacg agggaagcgg attatttgaa ctactcaatc cattcgggct gctgtgcggc
     5521 ctcgtctctg ccgcaatgct tgccacccac ggcgcggtct atctgacgct gaagacggac
     5581 ggccgggttc agcagcgtgc gcgggatttc gtcaagattg gcgccgtgct cacggtcgtt
     5641 ctatttgtct tggctggcct gtgggtgtgg cttcgaatcg agggttacgc catcaccagc
     5701 acggtgatcg gcgacgggcc gtcgaatccc ctcatgaaga cggtcgtccg ccaaccgggc
     5761 caatggctcg ccaattacgg agcgcatccc tggatgatcg tagcgccttg cctcggcata
     5821 tttggtccgt tgctcgcctt gctcttcacc acggctcaga gaggcggact tgccttcatt
     5881 gccagcgcgc tcggcattct tggtatcatt gcgaccgcgg gcgtaagcat gttcccgttc
     5941 ctgatgccgt cgaatgtcgc gccgatggcg agcttgaccg tgtgggatgc atcgtcgagc
     6001 cagctcactc tgttcgtgat gctgatggcg acgctgatct tcctgcctgt cgtactagtc
     6061 tacacatcga ttgtgttcag tgccctgcgc ggggtggtga ccgcgagcca aatcgagggc
     6121 aattcgccga atctctattg aggaggtagc atgtggtact ttacgtggat tctgggagtc
     6181 gccttcgcat gcgctttcgg cattctcaat gccgtatggc atgagcatca cctgccggac
     6241 gagacgatcg acgcttagaa taagcgcaat ctcccttgct gcgatgatga atgggcatca
     6301 tggcgcgcgc gaccaagtga ccctggtggg tttcgccctg acgaaacgct cgccaacaac
     6361 ggccgcaaga ttccacgaca agattttccg tccgagacgg cgcgctggct caacagcgcg
     6421 ctgaagccgc cgtaaagccc cgcgccgcgc ttcatttgcg gccaagaatt cccgttcacg
     6481 ctttcgcgag gcagtcctgg ccaggccttg aaccgtttgt gatcgtttcg cgctgcgaaa
     6541 gatcgaactg gagcgcaccg caacgcgacc aaggcacagg accgcaaggc cggggcgcca
     6601 acgcccgcca acgcgggtcc gatctcgcga caatgcgaca gttgcccttg tccctttgag
     6661 gatagggcac cgcttggtcg ccaaaagttc aaagggtggg aatgacgaaa tccagaggaa
     6721 tcggtagagg cgggccaagg ccgggatcgg gtcgaaagcg gaaagcgatc acatcgcgtc
     6781 tggcagcgct caagaacgtg ctggcggcaa tgctgagtag cgacgcctcg aatttcagcg
     6841 atgccgatcg gcggttcgtg cgggcgatga tcgcgttggg cgcgccgaac aatgcgaccg
     6901 ccggcgcatt cggcatttct gaagttgagt tgctcgcgaa atttccggac gagctgcaat
     6961 ctcgcccgcg cggcaccgcg cgtgccgatt aaggcttctt tcaggctcga ttgaggcttc
     7021 gtcaatcaca gaaaattgtc atctgccaac aggtttggaa gtcatccagt gtgacctctc
     7081 ggtgacagct ttttgagcaa aagctgtcta tctccgcgcc acggttgcag ccggcacgcg
     7141 gctgatccga aatcatctct ggagaataaa cttaatgaag aagcttctac tcggtactgt
     7201 ggcgctcgtc gctctcggcg cgaccgtgcc agcactcgca gccgatctcg ccgcgcgtcc
     7261 ctacaccaag gctcccgctt acgcacagcc gatctacaac tggaccggct tctacatcgg
     7321 cggtcatctc ggcggcgcgt tcggcggcga caatagcttg acgggcagcg atggccgctt
     7381 cctcggcggc ttgcagggcg gcgccgatta tcagttcgct ccgaactggg ttgtcggtat
     7441 cgaagcccag tactcctggc tcgacaacag caacaacggc gtgctgttcc ccggcggcgt
     7501 tctcgtcacc tccaacagcg accagcttgg atcggtcacc ggccgcctcg gctacacctg
     7561 gggcccggcg ctgctctacg ccaagggcgg ttatggatgg aaggatggca acaacctcaa
     7621 tgtaagcgtc ggcggcgtgc cgcaggcgtt cacgaccaac ggcaatcatc gcgacggcta
     7681 caccgtgggc gctggcctcg agtacatgtt cgcgccgaac tggtcggcca aggccgaata
     7741 ccagtactac aatttcggcg acaccacatt cacgaccggc cccgccgcga tcgtcggcgt
     7801 ccgattccgt gacgacgagc atacggtgaa ggccggcctg aattaccgct tcaactgggg
     7861 ccggcccgcc gtcgccggat actgatcgcg gcaatcacaa ccctgaaaag gccggccttt
     7921 gcgccggcct tttttgtctt gattggcaaa aatccagcaa atcctgccaa tgctcccgat
     7981 tttattaacc gggtaacgcg atactgccgg tcgcgattca gacggacggc ttgggcgggg
     8041 gcgacatggc gggcctattc acgatcggta aacctggttg gcgctcgcga ttcttggcgc
     8101 tgcaatgggg cgtcagggcg agcctgttcg cagccttcgc cgtaatcgcg ggcatgggtc
     8161 tcctcatcag cgccggcgcc ggcatggtac tgggacatct cggcgagcgg atggtcgatc
     8221 tgagcgcacg ggacattccc cgtctcgcgg ccagcctgca gctttccgcc cagagcgcaa
     8281 gccttgccag tcaggggccg gcgctgttgg cctcgcgcag cgaggaggcg ctcaacgaac
     8341 gcgccaaaaa aatgaaggaa acccaggcgg tcacgtttga caagctcggc gaggtgatcg
     8401 aactcggcgc cgacagggcg gttgtcgccg ctctcaccga aaccgtaagg aacatcgacg
     8461 agaccatcaa aagcctggtc gcggccgccc gcgagcggct ggaagtcgtc gcgcagcatg
     8521 agaagcaata cgatgcgctg cgcagggcac aggcggagtt tgttgccgcc gccaaccctg
     8581 cgatgctgga cgcccaaatc cagatcaacg ccatcctcgg ctccgccatg ctttctcagg
     8641 atgatgcaat ccaggtggcg cggaccatcg accagctcgg caacgccgtc gccagcggca
     8701 acctgatggc atccgagatg accgcagccc tctcggcaag cagcagcgct acgctcgaag
     8761 ccatcgaaaa ggacttcaag tcggcgcagg cccgcctcaa gtcgaacctc gatttgctgc
     8821 cgcaaaactc cacgaccagg tcgctcaagg acgctgcgct gaaattgctg gcgctgggcg
     8881 agggcaagag cggggtcttt aaacttcgcc agaaggaact ggacgccaac gattacggtc
     8941 agctcgtgct cgacgaaacc cgcaagctca atgtcggtct tggcatcagc gtgcagcagc
     9001 tggtcagcgg cgtgcagagc gagaccgacg gctcgacctg gcaggcccgc aaggagatct
     9061 cgctcgcgac cacggtgatg ctggcgctcg gcgccctcac cctggtcggt tccgtgctgt
     9121 tcgtctggct ctatgtcggc cgcagcgtcc tgcgccggat cggccatctg caacgctcga
     9181 tgcagcttct gtcggacggc gacctcgagt cggaaatcta tcgctcgacc cagcgcgacg
     9241 agatcgcgtc gatggcgaat tcactgcaga ttttccgcga gagcatgatc gaggcccgcg
     9301 cgctctccgc cgatcaggac agggaccgca tcggcaagac cgaacgcgcc tcccgcatgg
     9361 agacccggat tgccgaattc gaggcaaccg tccgcaccgc gctggacggc ctgcagaaat
     9421 cggccaattc catgcaggcc acggcgcaaa gcatgtcggc gaccgctgac cagtccagcg
     9481 cgctggtgaa cgcggtggcc tccgccgccg aggaaacctc ggtcaacgtg cagacggtgt
     9541 cggccggcac cgaggaattg tcctcgtcga tcgccgaaat cggccgccag gtggtcactt
     9601 cggcgcagat cgcccgcaag gccgtcgaag aagcaggggc aaccgacgcc accatgcagg
     9661 gtcttgccga caacgccagc cgcatcaacg tcgtggtcga tctgattcag gtgatcgctt
     9721 cgcagaccaa cctgctcgcg ctcaacgcca ccatcgaagc cgcgcgcgcc ggcgaagccg
     9781 gccgcggctt tgcggtggtc gcctccgagg taaaaagcct cgccaaccag accgcgaagg
     9841 ccaccgacga aattcgcgca cagatcgcca gcatgcagca ggtgacgacc tcggcggtcg
     9901 gcgccatcag gaacatcggg cagaccatcg gcgagatcaa cgacgtcacc accgcgatcg
     9961 cggcggcggt ggaggagcag ggcgcggcga cgcgcgagat cgcccgcaac gttcagcacg
    10021 ccgccggcgg cacagccgag gtttccagca acatcgtcgg cgtctccacc gcttccgccg
    10081 aagccggatc agccgccggc gaggtgctga gcgcctccag cgcgctgcgc cgcgaagccg
    10141 acgttctccg cgcggagatc gacgccttcc tgagcaacat ccgggcggct tagaagcccg
    10201 agcggcaact agtcggattg gtcattccgg gatggtgcgt aagcaccaga cctcagatgt
    10261 gcaattgcac atctgggaat ccttggatac cgggttctat gcttcgcata gccccggaat
    10321 cacggcttgc tatgaatttt gcccaaagcg gcacctgttt tttcggctgg cgttgcgccg
    10381 aatgtgcggc taagccgata gcaggacatt gcgacgcaaa acgatgcact caagaacaga
    10441 caccgttcac tcctcggccg aggcgctgcg atacccctgg gaaaaccatc ccggtccgga
    10501 tcaggccgtc gaggtgatgc cgggcgtatt gtgggtccgg ctcaaattgc cgttccgcct
    10561 caaccacgtg aacatctatc tgctcgccga cggcgagggc tggacgatgg tggattccgg
    10621 attcggcaac gaggaatcga tcgcggcctg gaccgcgctg ttcgagggcc cgctggctca
    10681 cgtcaagatc acgcggctga tcgtgaccca ttcgcatccc gatcatgtcg gtctcgctgg
    10741 atggatcgtc gagcgcttca gctgcccgct gcatatgtcg caagtcgaat atctgcaatc
    10801 ggtctaccat cagaaccgcg gcaccgaaga acgccgaaac gcgcagcggc tgttcttccg
    10861 ccgccacggc atggacgaga gcctgaccga caaattgctc ggccggggcc aggattattt
    10921 gaagcgggtt tcgatcctgc cgccgtccta tcgccgcatc tcgcatggcg acgaaatttc
    10981 catcggcagg cggcgcttca aggtcatcac cggcggcggc catgcgctcg accaggtgat
    11041 gctgtattgc gccgccgaca aactgttcct gtccgccgac caggtgctga gcaagatttc
    11101 acccaatgtc agcgtctggg cggtggagcc cgaccagaac tcgctcgggg agtatctcgc
    11161 ctcattggcg agcctcacca cgaccctgcc ctatgacgtc atcgtgctgc ccggccacgg
    11221 cgtgccgttt tacggattga agacacgcat caagcagttg gccgatcatc acgaggatcg
    11281 ctgcagactg atcgccgagg catgccagga ggtgccgaaa acatccaagg aactggtgcc
    11341 ggtggtgttc cacaagcacg cactcgacgt gcaccagatg ggatttgcgg ccggcgaact
    11401 gatcgcccat gtcaattaca tgctggtcga ggggcgtctg accgccgagg aagccgacgg
    11461 cgtgctccgc ttcaggacga cgtgacgcgc ttttgaagca ccctaaaggc gcagtcccgt
    11521 ccacgggacg tagcacagca tgcacgttgt cacgcgcgct tctcgagcca taacgatacc
    11581 ccgcttagct tcctgatcag agcgatcagg ttgccgactc cggctcccga atggaaggca
    11641 ccgccgcctg ctcgcccggc ggccctcgcc tgaccgaccc aaaccagctg ggcgacggcg
    11701 cggaagacct catagtagag cacggcctga tcatcgagcg gccggagctc ccggtacgtg
    11761 cgctcgtagc gtcgtagagc cgcgccgata atcgcgcgtg ccgtaacacg caatgcccat
    11821 ggcagaggaa gcggaacggc ggacatgttg gcgatcgctg aacccacatc catggcgggc
    11881 tctgcgatga ctgcattggc ccagtcgatg accccggtgg gctcattgtt gtcggcaagg
    11941 atgttgagcg ggtggaaatc gccatggcag atcgccgcgt ctcgcgcctt tgaaggtcga
    12001 tgatcgtcca gccaggcaag gccgggttgc agtccggtga gagggcaccg cccgacaatc
    12061 gccctgagcc gtgccaactg gccctcgaag gtaatagctc ccgcgtcgat gcccgccgcc
    12121 gttactgccc gcagcagggt ttcggccggc aattgatgca ggcggatctg catatctacc
    12181 cattggtcga ttatccgggc gaggatggcc ggaaggttga acagcaactg cagctgtccg
    12241 acaagggaag tgccggcgcc caaaccctcg atatcatggg ccagggtttg ccccgacagt
    12301 cgggtcatga ccatgaaggg tccgccgagg aggtcctgat cgctttccgt cactatcacg
    12361 cggggtgcgg gaaagcccat ttcggcgaga gtattctgga caatggtttc cagcttcacc
    12421 cgcgccggat cggcgttggc atgactgagg cgcaggatca acggccccac caaaggaggg
    12481 gtaacacgat cgagtgtgaa gccaaaaatc gcagcgtcaa agccgccctg aatccgcgtt
    12541 ggcccggcta gatacaccgc gtctgggcac ccgaaggcgt gcgccagatg atcgagcagc
    12601 cgcctggcca cttctgcgtc attgaacggg tgcatcgcga tgtacttccg ccacctcaac
    12661 gccaatcacg gttatcactc ccaaacgccg ctgtcccgtc acgaacccac gcagacaata
    12721 ggttggtatt gagcgtggag agtccggtcc gggtcaaatc gctcccgaaa cggcatgtcc
    12781 gaaccacgtc cgttccagct gatagcgacc gcatcgccga cggactgccg ctgcggaatt
    12841 gccaagcggc ctgcggccat ccatcatggg cgccaatggg agccggcacg atgttctcaa
    12901 gccgggctgg ggaggacgac aatgcctgac gtattgaatt tgatcagccg catcgtgttg
    12961 tcggcggtgt tcattgttta cgggtatctg gcgtttgccg atgtcaaggc ctgggtcaac
    13021 ttcaatcaag cggcgctgaa gcgcttcttc gacgtcgtcg ccggtggcat ggcgcccccg
    13081 acctggttcg gctatttgat cgcgacgatc cagctggtgg gcggacttgc aatccttgcc
    13141 ggtttcaaga cccgctgggt cgcttatgca ttcgtcgctt atctgctctt cgtcgagttc
    13201 tttgcccaca atttctggga catgcaggga ggggcgcgcg gcgccaacat ggcgcatttc
    13261 tacaagaacc tcgccatcat cggtggtttc ctgctgctcg cgctgcaagg cgcgggacgc
    13321 tattcggtgg atgggcaaat atcccgcgcg tgaacctgga cactggcggc gcccgcacgc
    13381 gctttcaacc ctcaacgtca gcccatggac gggttcgctg ggccgaaccg ccgccaacgg
    13441 cgcatggatt taggatcaag ttttgttgcg cgcatccagc attgccaaac cccgattctg
    13501 cctcgacatc aatcctggaa aggctctaaa tacgggttcc gggcctccag ttccagcttc
    13561 cgtttcaggt ttttgcgatg gattacaaca ggttctttac gaccgccctc aaccgcctgc
    13621 atgatgagcg gcgctaccgg gttttcgccg acctcgagcg gatcgccggc cgattcccgc
    13681 atgcggtgtg gcattccccg aaggggcctc gcaacgtcgt gatgtggtgc tccaacgact
    13741 acctcggaat ggggcaacac cccaaggtgg tcggcgccat gatcgagacc gccacccggg
    13801 tcggcaccgg cgccggcggc acccgcaaca tcgcgggcaa ccatcacccc ctggtgcagc
    13861 tcgagcagga attggccgac ctgcacggca agcaagcggc gttattgttc acctcgggct
    13921 acgtctcgaa ccagaccggc atcgcgacca tcggcaagct cattccgaat tgcctgatcc
    13981 tctcggatgc gctcaaccac aattcgatga tcgaaggcat ccgccagtcc agctgcgagc
    14041 gccagatttt tcgccatagc gacatggcgc atctcgagga attgctgcgc gccgccggcc
    14101 ccgaccggcc aaaactgatc gcctgcgaga gcctgtattc gatggatggc gatatcgcgc
    14161 cgctttcgaa gatctgcgat ctcgccgagc gttatggcgc catgacctat gtagacgagg
    14221 ttcatgcggt gggcatgtat ggtccgcgcg gcggcggtat tgccgagcgc gacggcgtca
    14281 tgctccgcat cgatattctg gaaggcacgc ttgccaaggc gttcggctgc ctcggcggct
    14341 atatcgccgc aaattccgac atcatcgatg ccgttcgttc ctatgccccc ggcttcatct
    14401 tcacgacctc gctgccgcca gcgatctgtt ccgctgcgac cgctgcgatc cggcacctca
    14461 aaacgtcgag ctgggagcgc gaccgccacc aggatcgcgc cgcccgcgtc aaggcgatcc
    14521 tgactgccgc cgggctgccg gtgatgtcga gcgacaccca tatcgtgccg ctgtttgtcg
    14581 gcaaccccga aaaatgcaag cgggcctgcg acatcctgct ggaagagcac ggcatctaca
    14641 tccagccgat caactatccg accgtcgcca agggcaccga gcggctgcgg atcacgcctt
    14701 cgccctacca cgatgacgga ttgatcgatc agcttgccga agcgctgctt caggtctggg
    14761 agcgcctcgg tatgccgctt cacgagaaat ccctggcggc cgaatagtcc ctatcctctc
    14821 accctgccgc gaaaccggcc cctgggccgg cgacatggcg atgctttgcc ggttcaatcc
    14881 ggctatactc cctccctgcg acggcggtgg cttgcgctag cccgggtcaa tgcgcacgcc
    14941 acgatggtta agggagtaag cagcgatgct gcacgactgg ggcgtgatcg cggccgcatt
    15001 cggctacatc gggttcctgt ttctggttgc gagctatggc gaccgcatgt cgccgacgca
    15061 gcgcggccgc gccggcacgc tgatctatcc gctctcgctg gcaatctatt gcacgtcgtg
    15121 gacgtttttc ggttcggtcg gattcgcctc ccgcaccagt gtcgattttc ttgcgatcta
    15181 tatcggcccg atcctgatga tcgggctctg cacgccgctg ctgcgccgcg tgatccagct
    15241 tgccaaatcg cagaacatca cctcgatcgc cgatttcatc gccgcgcgct acggcaagag
    15301 ccaggcggtt gccgcgacgg tggcggtgat cgcgatcatc ggatcggtgc cctacatcgc
    15361 gctccagctc aaggcggtgg cgtcgtcgct cgaaaccatc ctcagcgaag accaggccat
    15421 cgcctctgtt ccgatcatcg gcgatatggc gctgatcgtg acgctggcga tggcagcctt
    15481 cgcggtgctg ttcggaaccc gccagagcga cgcgacggaa catcagcatg gccttatgct
    15541 ggcggtcgcc accgaatcca tcgtgaaact ggtggcattt cttgcggccg gcatatgcgt
    15601 cacgttcttg ttgttcggtc cgttcgaact gatcgagcgc gcgatgaaga cgccggaagc
    15661 ggtgcgcgcc atcaactaca cgccatcggc cggcaatttc ctgaccatgg tgctgttgtc
    15721 gttttgcgcg atcatgctgc tgccgcgcca gtttcacgtc agcgtggtcg agaattccag
    15781 cgacgccgag gtaggccggg cacgttggct gtttccgctg tatctggtcg ccatcaacct
    15841 gttcgtgatc ccgatcgcta tcgccggtct ggtgaccttt ccgttcggcg cggtcgacag
    15901 cgacatgttt gtgctggcgc tgccgatcga gggcaatgcc gtattgctca gcgtcggcgt
    15961 cttcatcggc ggcctgtcgg cggcaaccgc catggtgatc gtcgaatgcg tcgcgctttc
    16021 gatcatggtc tcgaacgaca tcgtggtgcc gctggtgctc cagcgcggtc cgcaatcgcg
    16081 cgcgggcgag cgggactttg gcgacttcct gctcagggtt cgccgtttcg cgattttcgc
    16141 catcatggtg atggcctatt tttactatcg tgcgctgggc aacgcgcaat tggcgtcgat
    16201 cgggctgttg tctttcgcgg ccatcgcgca gcttgccccg gcttttttcg gcggcctgtt
    16261 ctggcggcgc gcgaccgcgc gcggcgccat ggcgggcatg ctggtcggcg ttgccgtgtg
    16321 gagctatacc ctgtttattc cgagcttttt gggcggcagt cccgccggcc tcttgctgct
    16381 gcagcacggg ctgttcggca tcgaagcatt gcgcccgcaa gccctgttcg gcgccgatct
    16441 gccgccgctt ctgcatggcg ttctgtggtc gctctcgctt aacctgctga cctatatcgt
    16501 gctgtcgctc gcgcgcgagc cgtcctcgat cgaacggttg caggcggacc tgtttgtgcc
    16561 cgatacgctc gcgccgacga cgccgacgtt ccgccgctgg cgcaccacgg tgacggtgca
    16621 ggatattcag agcacggtgg cgcagtatct cggccccgat cgcgcgcatc atgcgttcga
    16681 agcctttgcg gtcacccaca acacccgcct cgacccggcg gcgccggccg atttcgaact
    16741 gctgcagcat gcggagcacc tgatcgcgtc gtcgatcggc gcggcctcct cgcgtctggt
    16801 gatgtcgctg atgctgcgca agcgtaccgt ctccgccaag gccgcgctca aactgctcga
    16861 cgattcccac gccgcgctgc acttcaatcg cgagatcctg cagaccgcgc tgaaccatgt
    16921 acggcagggt atcgcggtgt tcgacgcgga gttgcagctg atctgttcaa accggcagtt
    16981 cggcgagatt cttgggttgc cgctgcacct cgtgcagatc gggattccgc tccaggaaat
    17041 cctggaattc atggggtcga tcagccccgc gggtctgggc gacagccacg cgttattgga
    17101 aaggcggctg gccgcctata ccaccgaagg caagccgtat ctggaacgcc ttcccgaccg
    17161 tcacatggtg atcgaggtgc gcagcaaccg gatgcccggc ggcggactgg tcatcacctt
    17221 ttccgacgtc acgccgtcct tcgaggccgc cgaagcgctg gaacgcgcca atgccaccct
    17281 ggaaaaacgc gtgcgggatc gtaccgagga gctcacccgg ctgaattcgg aactggcgct
    17341 ggccaagagc accgccgagg acgccaacat ctcgaagacg cgttttctgg cggcggcgag
    17401 ccacgacatc ctgcaaccgt tgaatgcggc gcggctctat gtgacgagcc tggtcgagcg
    17461 ccaaaacggc ggcgaggatt ctcgcctggt cgaaaacatc gacgactcgt tggaggcgat
    17521 cgaagaaatc ctcggggctc tcctggatat ttcccggctc gacgccggcg ccatgacgcc
    17581 ttcgatcacc agtttcaaga tcggcgacct gatgcgctcg ctggaaatcg aattcgcgcc
    17641 gatcgcacgc gccaagggac tgaagctgac tttcgtgccc tgctcgctgc cggtggcatc
    17701 cgatcgtctg ctgctgcggc ggctgatgca gaacctgatc tccaacgcga tcaaatatac
    17761 cccgcgcggg cgcgtactgg tcggttgccg gcgccacggc cagtcgctgc agctcggcgt
    17821 ttacgacacc ggcgtcggca ttcccactct caagcgcagt gagatcttca aggagttcca
    17881 ccgcctggag cagggcgcgc ggattgcacg cggccttgga ctgggactct cgatcgtcga
    17941 gcgccttgcg cgcgtcctca accacggcat tgcactcgac tccaatgtca gcggcggctc
    18001 gtttttttcg gtaacggtgc cgatcgcaaa agcgatcaat tacaccgcgg ccgtcaccag
    18061 cgcgacctcg ctttccagga cgccgatgag cggtgccctc gtcgtttgta tcgagaacga
    18121 cccggcgatc ctggacggca tgaggacgct gctgaccgcc tgggacgcca aggtgattgc
    18181 ggtggccgat cccgaggccg cgatcgggcc gatcgaggcc gcgggcggac aggttaccgg
    18241 tcttctggtc gactatcacc tcgaccgcgg caacggcgtc gccgcgatcc gggacattcg
    18301 ccgccggttc ggcgagaata ttccggcgat cctgatcacc gccgatcgca gtccaaatgt
    18361 acgggcggcg gcgcgggaag agaacattgc ggtgctcaac aagccggtca aaccggcgtc
    18421 cttaagggcc ttgctgggtc aatggcgcag ccagcagatg ctggccgcgg aataggcgag
    18481 ctgatctcgt gagcggatct attccggtgg gggggcgtct tgccgccact ggccgccggc
    18541 aattttcgcc gcggcgatca ccgcctgggt gcggctctcg acgccgagct tttgcaggat
    18601 cgccgataca tgcgccttga tcgtcgcttc ggacacgcca agctcatagg cgatctgctt
    18661 gttgagcagg ccttccgaca gcatcatcaa caccctcacc tgctgcgggg tcagcgtcac
    18721 cagccggtcg cgcaggcgag ccatatccgg atcggcggcg gccgatagat cggtgtccgg
    18781 cggtatccag acgtcgccat ccatcacctt cacgatcgcg tcgcgcaacg tctcg
//
LOCUS       DATNOI010000410         6996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1281223,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000410 DATNOI010000000
VERSION     DATNOI010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281223"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="VN968_16445"
     CDS             complement(<1..1047)
                     /locus_tag="VN968_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HXL30859.1"
                     /translation="MKTRAAVAFEAKKPLEIIELDLEGPKAGEVLVEIKATGICHTDA
                     YTLDGLDSEGIFPSILGHEGAGIVREVGPGVASVKPGDHVIPLYTPECRQCKSCLSQK
                     TNLCTAIRATQGKGLMPDGTTRFSYQGKPIFHYMGCSTFSNFTVLPEIAVAKIREDAP
                     FDKSCYIGCGVTTGVGAVVNTAKVTPGANVVVFGLGGIGLNVIQGARMVGADKIIGVD
                     LNDGKEEWGRRFGMTHFINPKKIDGDIVAHLVALTDGGADFTFDCTGNTTVMRQALEA
                     CHRGWGVSVVIGVAEAGKEISTRPFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMNGK
                     IEIDPMITHVLKLEE"
     gene            complement(1436..2056)
                     /locus_tag="VN968_16450"
     CDS             complement(1436..2056)
                     /locus_tag="VN968_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL30860.1"
                     /translation="MRVLIVDDHPIVASGCRALFAGNPEIVILEASDAESGERAFVAE
                     QPDVCVLDINLPTVSGFELARRILARVASARIIMFSMNDDPIFAARAIEVGAKGYVSK
                     SGDPCDLVEAIREVGKGGTYLPSAIAQSIAFAGPALAQSPLSKLTSREIEILRLLSAG
                     KSLSEIAWLVHSSYKTIANTSSIMRQKLGVRTSAELVRLAIENSLT"
     gene            2267..3643
                     /locus_tag="VN968_16455"
     CDS             2267..3643
                     /locus_tag="VN968_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXL30861.1"
                     /translation="MWQKLSLRARLNMLLALVLTLGLAVNIARLVLEAAPRVQAEDQS
                     VIRLAREFIETLVVGLNETADPDARLDQIVQDLNRLRHVSIARQDDASAARSPTTDRS
                     SDDREAYSLPAWFVALVHPEKTAVNVPISIHEKAGSLVITSHPNDEMAEIWDGIVTQL
                     QVGSAIAVALFLITMMVVSRALAPIETLSQAMTKIESGSYDTRVKPDGPPELAAICTK
                     LNHLAGALGDAVEDKRRLAERVVSLQDVERKEIARELHDEFGPYLFALRAHASALTRI
                     VDARVPDTDALRRHGGAILEQVNALQQFNRRVLEKLRPVGLTELGLREALGVLLRLWG
                     EAHPGVVIETAISPSLGETGETADLTIYRTVQEALTNVFRHAGATSVNVTIEPTDQLS
                     GMKRGGHGCALVRVCDNGSGLSPDHRLGLGLTGMRERILALGGTLTVASSGSGVTVEA
                     VVPKGGRC"
     gene            4032..5036
                     /gene="exbB"
                     /locus_tag="VN968_16460"
     CDS             4032..5036
                     /gene="exbB"
                     /locus_tag="VN968_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tonB-system energizer ExbB"
                     /protein_id="HXL30862.1"
                     /translation="MLLTIVVSLMSFTALAEQPSVGAEGASGQPVAAVAAPARPTPAR
                     DAGQGVPPSEAEGKAPRPEPQSALSSSSPASPQAQSSSVVTSAVGPLPDALSPWGMFV
                     QADVVVKAVMIGLALASVATWTVLLVKTIQLRAAKHEGLRALATVLSARSLLEAARQI
                     ADQRNAVGRLLAAAEDEVEKSRGLPAEGIKERIAWQLERIEAAAAQRIGRGIGILATV
                     GAVAPFVGLFGTVWGIMNSFIGISRAHTTNLAVVAPGIAEALLATAFGLAAAIPAVVI
                     YNAFARSTGNYRAVLADSSVAILRLLSRDLDRNALSIDARRGPRTADGTVLSIADSAG
                     "
     gene            5040..5465
                     /gene="exbD"
                     /locus_tag="VN968_16465"
     CDS             5040..5465
                     /gene="exbD"
                     /locus_tag="VN968_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HXL30863.1"
                     /translation="MTIRLAKRDKEYAEVHEINVTPFIDVMLVLLIIFMVAAPLATVD
                     VPVNLPSASAEPPARPDKPIFVTFKADHSLAVGDEAVTRADLAAALDAATGSDRTQRV
                     LLRADQASSYGEVMELMNLLRNAGYVKVALVALEIRSGP"
     gene            5471..6382
                     /locus_tag="VN968_16470"
     CDS             5471..6382
                     /locus_tag="VN968_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXL30864.1"
                     /translation="MTVDPFKREQARDAALWTGAALVVAALHVGLAVAYLLLKPAPEG
                     RAEAPVIDVAFMPAASAPAPAEPETPPAEPTPLVEQSDLGPPKNEPVAAREAVTEPEP
                     PKVKTESAPPPEPVPPPVALQFPEPAAEPAHPDQVAVMPPPSKPAEPAPQAKPVTVPE
                     RSAHKPAADEKAEREKHKTAPPKPAAAPASKPAQLAMAPNSGADSEGARAGRASWESE
                     LAAHIRRYATYAANGSRESGTVRVGVSIDRNGRLLSRRLAGSSGSPVLDSAAMTVIER
                     AQPYPRFPPGMSQAQIALTIPLHLRPQ"
     gene            6629..>6996
                     /locus_tag="VN968_16475"
     CDS             6629..>6996
                     /locus_tag="VN968_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HXL30865.1"
                     /translation="MTKLKALKSALLLSCAVLPGVASAQETLQTIEVVGVSPVPGGEI
                     DREKVPSNVQTIDASDLDHAKTPSLLDGMVQSLPGVSLSDQSGNAFQRSLDYRGFTAS
                     PVPGTVQGLAVYQNGTRINEA"
BASE COUNT         1309 a         2139 c         2196 g         1352 t
ORIGIN      
        1 ctcctccagt ttgaggacgt gggtgatcat cggatcgatc tcgatctttc cgttcatgta
       61 ccagtcgacg attttcggca cgtcggtgcg gccgcgggcg ccgccgaacg ccgtgccctt
      121 ccagacccgc ccggtcacca gttggaacgg ccgggttgaa atctccttgc cggcttccgc
      181 gaccccgatc acgaccgaga cgccccagcc gcggtggcag gcttccagcg cctggcgcat
      241 cacggtggtg ttgccggtgc agtcgaaggt gaaatcggcg ccgccgtcgg tgagcgcgac
      301 cagatgcgca acgatgtcac catcgatctt tttcggattg atgaaatggg tcatgccgaa
      361 gcggcggccc cattcctcct tgccatcatt gagatcgacg ccgatgatct tgtcggcgcc
      421 gaccatccgc gcgccctgaa tgacgttgag cccgatgccg ccgagcccga acaccaccac
      481 gttggcgccc ggcgtgacct tggcggtgtt gaccaccgca ccgacgcccg tggtcacacc
      541 gcagccgatg tagcagctct tgtcgaacgg cgcgtcctcg cggattttgg ccaccgcgat
      601 ttccggcagc acggtgaaat tcgaaaatgt cgagcagccc atataatgga agatcggctt
      661 gccctggtag ctgaagcgcg tggtgccgtc gggcatcagc cccttgccct gggtggcgcg
      721 gatggcggtg cacagattgg ttttttggct gagacagctt ttgcactggc ggcattcggg
      781 cgtgtaaagc gggatcacat gatcgcccgg cttcaccgaa gcgacgcccg gtcccacctc
      841 gcgaacgatg ccggcgccct catggccgag gatcgaggga aagattccct cgctgtcgag
      901 gccgtcaagg gtgtaggcgt cggtgtggca gatgccggtt gccttgattt caaccaggac
      961 ctcgccggcc ttcgggccct ccagatcaag ctcgatgatt tcaagcggct tttttgcttc
     1021 gaacgcgaca gcggcacggg ttttcatctg tttcatctcc ctcgagagct tggcttatcg
     1081 agttccttgg cagggaacga cgccaatttg cttcaattcg tgcgcggtga aaacgcgatg
     1141 tacgaacccc ccgatgacca gagcattgca cgctaacggc gaacgtcaag gtcggcgatt
     1201 ccaaacgtct cgatttttct atcggcggtg tcaggtagcg aagcggatgt gccgcgttgc
     1261 ggcgtaagct atttcttcgc ggcggcgaac aaaatctgct gcatcgcggc gcggaagtct
     1321 tgccgacctg ctcggtcatt gtaaaattat cggacggaaa agaggcggcc ggatggcgcg
     1381 cttggggcga cccatccctg tcgaaatatc ggaagccgga ttgtgcgggc ggggatcagg
     1441 tcaggctgtt ctcgattgcc agacgcacca gttcggcgga cgtgcgcacg ccaagcttct
     1501 ggcgcatgat ggaagacgtg ttggcaatgg tcttgtagga tgaatgaacc agccacgcga
     1561 tctcggaaag acttttccct gcgctgagca aacgcaatat ctctatttct cgtgatgtca
     1621 gtttcgacag cgggctctgg gccagcgcag ggccggcgaa cgcgatgctt tgggcaattg
     1681 ccgacggcag atatgtcccg cctttgccca cttcacggat ggcctcaacg aggtcgcatg
     1741 gatcgccgga cttggagaca tagcccttgg cgccgacttc gatcgcgcgt gcggcgaata
     1801 tcgggtcgtc gttcatgctg aacatgatga tgcgcgcgga tgcgacgcgc gccagaatgc
     1861 gccgcgccaa ttcaaagccc gacacggtcg gcaggttgat atcgagaacg cagacatcgg
     1921 gctgctcggc tacgaacgct cgctcgccgc tctccgcgtc ggacgcctcc aatatgacga
     1981 tctccgggtt tcccgcgaat aatgcacggc agcccgacgc cacgatggga tgatcatcaa
     2041 cgatcagaac acgcatcggc gttggtcccc aattcgcgcg tcagtttgtg ttgttgcatc
     2101 gcatcagatc gaaccacaga gaaatccgac gtcgcgacgc ttgcccgacc gggttatgca
     2161 tcgcaagcgt aattgctgta cggttcaatt agatagccgc cgtttttatc tgtcaacgct
     2221 cctcttgatg aggggcccgg cgtggcgcgg tattggacga aaagctatgt ggcagaaact
     2281 atccttgcgc gcccggctca acatgctgct ggcgctcgtt cttacccttg gtctagccgt
     2341 caacatcgcg cgcctggtac tggaggcagc gcctcgtgtg caggccgagg accaaagcgt
     2401 gatacgattg gcgcgcgaat tcatcgaaac gctggtcgtc ggcctgaatg aaacagcgga
     2461 ccccgacgcg aggcttgatc agatcgttca agacctcaac cggctgcgtc acgtcagcat
     2521 tgcgcgccag gatgatgcga gcgcggcgag atccccgacg accgatcgtt ccagcgatga
     2581 ccgcgaggca tattcgttgc ctgcctggtt cgtcgcactg gtccatccgg agaaaaccgc
     2641 ggtgaatgtc ccgatttcca tccatgaaaa ggcgggatcg ctggtaatca cctcgcatcc
     2701 caacgatgag atggcggaga tttgggatgg aatcgttacc cagctccagg tcgggtccgc
     2761 gatcgccgtc gcgcttttcc tgatcacgat gatggtggtg agccgggcgc tcgcgccgat
     2821 tgagacgctt tcgcaagcga tgacgaagat cgaatccggt agctatgaca cccgcgtgaa
     2881 accggacggc ccgcccgagc tggcagcgat ctgcaccaaa ctgaatcatc ttgcgggcgc
     2941 actcggagac gccgttgagg acaagaggcg tctcgccgag cgcgtcgtct cgctgcagga
     3001 cgtcgagcgc aaggagatcg caagggagct gcatgatgag ttcggacctt atctctttgc
     3061 gctgcgcgcg cacgccagcg cgctgacgcg gatcgtcgac gctcgcgtac cggacacaga
     3121 tgccttgcgc aggcatggcg gcgccatcct ggaacaggta aatgccttgc agcaattcaa
     3181 tcggcgggtt cttgaaaaat tgcggcccgt tgggctgacg gaacttggcc ttcgcgaagc
     3241 gctcggtgtt ctcttgcgcc tgtggggcga ggctcatccc ggcgtggtga tcgagaccgc
     3301 gatttcgcca tcgctcggcg agaccggaga gaccgccgac ttgacgattt accgcaccgt
     3361 tcaggaagcc ctgacgaatg tttttcgaca cgcaggcgcg acatccgtca acgtcaccat
     3421 cgaacccacg gatcagcttt cggggatgaa gcgcggcggc cacggctgcg ctttggtgcg
     3481 tgtgtgcgac aatggcagcg gattgagccc ggatcacagg ctgggcctcg gcctgacagg
     3541 catgcgcgag cggatcctgg ccttgggtgg tacattgacg gtggcgtcca gcggcagtgg
     3601 cgtaaccgtc gaggccgtcg ttcccaaggg cggacggtgc tagagagaag ttcgcgatcg
     3661 gactctggga cgttctccca gttttttcgg gaatttttcc cgacgaaatg atcatcttga
     3721 agcggggtgc aacaagcggc actctgttcg cgtgtcagat cgttttgcgg cttctgccgt
     3781 tactttgccg aggcaaaata tgacacgatt gcagcgtggc gtcgtcagcg atccatgatt
     3841 tctcgatccg ccaaattggc tcgcgagatc ggacggtcaa ggcacgccgc ttgcgaaacg
     3901 acaggggacc tggaatcaac caggatcgtt gttgcgtcaa gcgggaggag tataagaaca
     3961 tggataaggc gcggacccaa cagcgaggcc gaagacgggg cagacgaggt ctttcagctt
     4021 tgcgtttcga attgctgctg accatcgtgg tatcgctgat gtctttcacc gctcttgccg
     4081 aacagccttc tgtcggtgca gagggagcgt cgggacaacc cgtggctgcc gtcgcggcgc
     4141 cggcgcgccc gacacccgcg cgcgacgctg gccagggcgt tcctccttcg gaagcggaag
     4201 gaaaggcgcc gcgccccgaa ccgcaatcgg cactctcctc gtcttccccc gcatcgccgc
     4261 aagctcaatc ctcttcggtt gtaacaagcg ccgtcggccc cttacctgat gccctgtcgc
     4321 cctgggggat gttcgtccag gcggacgtcg tggtgaaggc ggtcatgatc ggtctggcac
     4381 tggcgtcggt ggcaacctgg accgttctcc tcgtcaagac cattcagttg cgagcggcca
     4441 aacatgaagg gctccgcgcg ctggccactg ttctctccgc ccgttcgctc ctggaggcgg
     4501 cgcgccaaat cgcggaccaa agaaacgctg taggcagact tctcgccgca gccgaagacg
     4561 aggtcgagaa gtcacgaggt cttcctgcag aaggcatcaa ggagcgtatc gcctggcaac
     4621 tcgagcggat cgaggccgca gccgcgcagc ggattgggcg cggcataggt atcctggcca
     4681 ccgtcggggc ggtcgcgccg ttcgtaggtc ttttcggtac ggtgtggggt atcatgaaca
     4741 gtttcatcgg catttcacgg gcccacacca cgaaccttgc cgtcgttgct ccgggcatcg
     4801 cggaggcgct gttagcgacg gctttcggtc tcgccgcagc gatccctgct gtggtgatct
     4861 acaacgcctt cgcgcgctcc accggcaact atcgtgcagt gcttgccgat tcgtcagtcg
     4921 cgatcttgcg cttgctaagc cgcgatctcg accggaacgc tttgtcaatc gatgccagac
     4981 gcggcccgcg gacggcggac ggcacagtgc tatcgatagc agattcggcg gggtgagcca
     5041 tgacgatccg gctcgccaag cgcgacaaag agtatgcgga ggtccatgag atcaatgtga
     5101 cgccattcat cgacgtcatg ctggttctcc tcatcatctt catggtcgca gctccccttg
     5161 cgacagttga tgttccggtc aacctgccgt cggcttccgc cgaaccgccg gcccgtcccg
     5221 acaagccgat cttcgtgacc ttcaaggcgg accactcgct cgcggtcggc gacgaagcag
     5281 tcacgcgggc cgaccttgcc gcagcgctcg atgccgcaac cggctccgat cgcacgcagc
     5341 gggtgctgct gcgcgccgat caggcctcgt cctatggcga ggtgatggag ctgatgaacc
     5401 tgctgcggaa cgcaggctat gtaaaggtgg cgctggttgc gctggaaatc cggagcgggc
     5461 cgtgagcgtg atgacggtcg atccgttcaa acgggaacag gccagggacg cggcgctgtg
     5521 gactggggct gcacttgtgg ttgccgcgct ccatgtcggc ttggccgtgg cctatctgct
     5581 tctcaaaccc gcgcccgagg gacgagccga ggcgccggtg atcgatgtcg ccttcatgcc
     5641 ggctgcaagc gcgcccgccc cagccgaacc cgaaactcca ccggccgaac caacgccgct
     5701 ggtcgagcag tccgatctcg ggccgcccaa gaacgagccc gtcgcggctc gggaggccgt
     5761 caccgaacct gagccgccca aggtgaagac agaatcggcg ccgccgcctg aaccggttcc
     5821 gccgcccgtc gcgctacaat tccccgagcc tgcagcagag ccggcgcacc ccgatcaagt
     5881 cgcggtcatg ccgcctccct cgaagcccgc agagccggcg cctcaagcga agcccgtgac
     5941 ggtccccgag cggtctgcgc acaagccagc cgcagatgaa aaggccgaga gagagaagca
     6001 caagacggcg ccgccaaagc ccgcggccgc gccggcgagc aagcccgcac agcttgccat
     6061 ggcgcccaat tccggcgcgg acagcgaagg agctagggcc gggcgtgcat cttgggaaag
     6121 cgaactggca gcgcacattc gccgctatgc cacctacgcc gccaatggca gcagggagag
     6181 tggcaccgtg cgcgtgggcg tctcgatcga tcgcaacggg cggttgttgt cgcggcggct
     6241 cgccggcagc tctggctcgc cggtgctgga tagcgcggcg atgactgtca tcgagcgcgc
     6301 gcagccctat ccgcgttttc cgcccggcat gagccaggcg cagatcgcgc tcaccattcc
     6361 gctgcacctg agaccgcagt gagagcggcg cggccggaat cagcgacgtc gcatgaagcg
     6421 gcttggacta ctgtaacttg cctgcttttc gctatcggga agatctcccg atagcgctgg
     6481 gacaaatccc tgttgcggcg ttcagagcca gccactagca atatttccat atatttaaag
     6541 gcgcaattcg accaatgtgc gcagttcaag gctcagggga tgctggcgag ccaagagtag
     6601 cacggtcgaa agtgaattgg gggacgtttt gaccaagctc aaagcactga agagcgcgct
     6661 tctcttgagc tgcgccgtat tgcctggggt tgcgtcagcg caagagacct tgcagaccat
     6721 cgaagtcgtc ggcgtatcgc cggtgcccgg cggagagatt gatcgcgaaa aagtgccgtc
     6781 caacgttcag acgatcgatg cgtccgatct tgatcatgcg aagacacctt cgcttcttga
     6841 cggcatggtg cagtcccttc ccggcgtctc cttgagcgac cagtccggta acgcatttca
     6901 gcgtagtctc gactatcgtg gcttcacggc ctcgccggtt ccggggacgg tgcagggcct
     6961 ggccgtttat cagaatggta cccggatcaa cgaagc
//
LOCUS       DATNOI010000411         2917 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2042243,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000411 DATNOI010000000
VERSION     DATNOI010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042243"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VN968_16480"
     CDS             complement(<1..953)
                     /locus_tag="VN968_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HXL30866.1"
                     /translation="MEKLPAQMTVVGISKPGGPEVLLPETRAVPVPGPGEILVKVLAA
                     GVNRPDVAQRSGAYPPPPGASDLPGLEVAGEVAAVGAGSTRHKIGDKVMSLVAGGGYA
                     QYCIAQDAQAMAVPPALSMQEAGATPETLMTVWHNVFERGGLKPGETLLIHGGSSGIG
                     TMAIQLAKAFGSKVIVTVGSKDKADACLKLGADRAINYKTEDFVTEVKTATDGAGVQL
                     ILDMVGGDYIERNYDAAAVDGRIVQIAVLGGPKATVNFARLMVKRLHHTGSTLRPRSN
                     ADKAAMVAAIEARVMPLVREGRIKPLIDSTFPLAKAADAHRR"
     gene            1051..1242
                     /locus_tag="VN968_16485"
     CDS             1051..1242
                     /locus_tag="VN968_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HXL30867.1"
                     /translation="MAIEDDDKPRKKVTHEIGQDLSLLSVEELAERIALMTSEIERLR
                     VAMTKKRASKDAADRFFKS"
     gene            1400..2506
                     /locus_tag="VN968_16490"
                     /pseudo
     CDS             1400..2506
                     /locus_tag="VN968_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          561 a          887 c          898 g          571 t
ORIGIN      
        1 cgccggtgcg cgtcggcggc cttggcgagc gggaaagtgc tgtcgatcag gggcttaatg
       61 cgcccctccc gcaccagcgg catcaccctg gcctcgatcg ccgcgaccat cgccgcctta
      121 tccgcattgc tacgggggcg cagcgtcgag ccggtatggt gcagcctctt gaccatcagc
      181 ctggcaaaat tgacggtggc cttggggccg ccgagcaccg cgatctgaac aatgcggccg
      241 tcgaccgcgg cagcgtcgta gttgcgctcg atgtagtcgc cgccgaccat gtcgaggatg
      301 agctggacac ccgcgccatc ggtggcggtc ttaacttcag tgacaaaatc ctcggtcttg
      361 taattgatgg cgcggtcggc gccgagcttc agacaggcgt cggccttgtc tttcgacccg
      421 acggtgacga tcaccttgga accgaacgcc ttggcgagct ggatcgccat ggtgccgatg
      481 ccggacgagc cgccatggat caggagcgtc tcgccgggct tcagcccgcc gcgctcgaac
      541 acattgtgcc agaccgtcat cagggtttcg ggggtggcgc cggcctcctg catcgacagc
      601 gccggcggca ccgccatcgc ctgggcatcc tgcgcgatgc agtattgcgc atagccgcca
      661 ccggccacca gcgacatcac cttgtcgccg atcttgtgcc gggttgagcc cgcgccgacc
      721 gcggcgactt cgccggccac ttcgagcccc ggcaaatcgc tggcgccggg cggcggcgga
      781 taggcgccgg aacgttgggc tacgtcggga cggttgacgc cggcggccaa aaccttgacc
      841 agaatctcgc ccggtccggg cacggggacc gcgcgggtct cgggcaaaag cacctccggg
      901 ccgccgggct tgctgatgcc gacgacggtc atttgcgcgg gcagcttttc catgatttgt
      961 ccttgcgcaa cgcttcaaaa ttcgaagagg caatctgatt agccagccgc gctccgactg
     1021 gcaaccgttt cactgaccgg aggagaacct atggctatcg aggacgacga caagccgagg
     1081 aagaaagtca cccacgagat cgggcaggac ctgtcgttgc tgtcggtcga ggaacttgcc
     1141 gagcgcatcg cgctgatgac ttcggagatc gagcggctgc gagtggccat gaccaaaaag
     1201 cgcgcctcga aagacgctgc cgaccgcttc tttaaatcgt gagagccccc gattaattct
     1261 cactctgcgc aggtagccgt catggccggg ctcgtcccac gagcgtccgg cacggttcgt
     1321 gcttgaagag gcgcacggca ttgattctac tcgtttccag ctggttacga atcatctgga
     1381 cacgaaaggg atcaacgccg tgcggcatca gaatatcgtt tttcatgggc ttctgaagca
     1441 tattccgtgg tcgaccctcg atcagcttgt ggatcagtac aaagccgact gggatgggcg
     1501 cgtcgtaaag accaaggccc atctgatcgc gatgctgtat gcgcagtttt gcggcgcacg
     1561 gagcctgcgc gagattgaaa cgagcctgaa gagtcactcc ggcaagcttt accatctcgg
     1621 cggctgcacg atatcgagat cagcgctctc gaccgccaat gcttcgcgtc ctgtggaggt
     1681 cttcgcaggt ctgctttcgg tcctgatggc gcagttgcag gccggctatc ggcggaagat
     1741 cggcgattgc gttcggttga ttgattcgac cagtgtgcag ctgagcggcc tgagcggcga
     1801 ttgggcaacg ttttcggcgg gagtttgcgg cgccaaagcg cacgtcattt acgatccgga
     1861 tgccgatcaa ccgctgtacc tcatggtgac accgtcgaac gtcaacgaca tcacggccgc
     1921 caaggagatg ccgatcgagg cgggagcgac ttacgttttt gacctcggct attacgattt
     1981 tggttggtgg gcgaagcttg atcaggcggg ctgccgcatt gtcacgcgcc tgaagtccaa
     2041 cacgcctttc accgtcgtgg aggatcggcc ggtgtcgcca ggatcgtcgg tcctcagcga
     2101 tcggaccggg taccttccca aacggctcgc cgcgtcgcgt caaaacccga tggcgggtct
     2161 cgtccgtgaa attcgcgtca tgatcgagac cggcaaggtg ctgcgcatct tcaccaacaa
     2221 cctggaggca agcgcgcaag aaattgccga cttgtacaag cgtcgatggg ccatcgagtt
     2281 gttctttcga tgggtcaaac agaccttgaa gatcaaccac ttcttgggaa cctctgaaaa
     2341 cgccgttcgc attcagatca ccgtcgcgct gatcgccttc ctgctgctgc ggctggctca
     2401 cgacgccaac aggatcgtga tcagcccgct cgctttcgca aggctcctgc gaacaaacct
     2461 gatgcatcgg cgcgcaatcg ctgagcttct ccaagctacc ccacccccca agtcaccaca
     2521 gcccagattt gacttcggac cacacgcaac ccgcgcggcg caacggcgct gctatattcg
     2581 tgctcatgcc gacatggaga acgcggcatg ataagcacag accgtgccag acagccgtgg
     2641 acttgatccg ggcatccatc aaaatgaaaa attctcacaa gagacacgca acagagatgg
     2701 attgccgggt caagcccacg agcgtccggc acggtttgtg catttatgct gcctctttga
     2761 tctgagcaca attgcgagaa gcgcggcgac gatgagccgc tcgggtcgca tgcggcgcga
     2821 agtcgaatct gatctggtgt tggccgggtg ttgatggcga tctggattgg agaagctcgg
     2881 cgatctgccg tcgcagcatg aggtttgttc ggatgag
//
LOCUS       DATNOI010000412        12100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2417126,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000412 DATNOI010000000
VERSION     DATNOI010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12100
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2417126"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..135)
                     /gene="ffs"
                     /locus_tag="VN968_16495"
     ncRNA           complement(39..135)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VN968_16495"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            365..790
                     /locus_tag="VN968_16500"
     CDS             365..790
                     /locus_tag="VN968_16500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HXL30868.1"
                     /translation="MPSDAPAWSLHSRLKEDTIDIGDLPLCRVLVIKDANYPWLLLVP
                     RRHEAVEIIDLDEVEQAQLIAEISRVARALKEITKCDKLNIAALGNLVPQLHVHVIAR
                     RSSDAAWPRPVWGAVPPLAQDAEEVQNFISALRRKIWLG"
     gene            795..1745
                     /gene="nudC"
                     /locus_tag="VN968_16505"
     CDS             795..1745
                     /gene="nudC"
                     /locus_tag="VN968_16505"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HXL30869.1"
                     /translation="MSAFDSFPLGRPAFVSNILDRAAHLRSDDAKLMALEGRRDARAY
                     VVYRDSLVLKQEADGPRALLSIDEALKFGANPGTIFLGLRDGAAVFGMGIPAAAVEKL
                     LARSDVAVTELRGMAMQGVVPPDQLSAIAMAKSMVSWHQRHGYCANCGTRTAMKDGGW
                     KRECPNCKAEHFPRTDPVVIMLVTSGDKCLLGRQKQFPPGMWSCLAGFVEAAETIEDA
                     VRREILEESGIRCTDVNYYMTQPWPYPSSLMIGCTARATNEDIVVDRAELEDARWFDR
                     AEAALMLKRQHPNGLAGPHPFAIAHHLVGRWLQTDQRPGA"
     gene            1828..2790
                     /locus_tag="VN968_16510"
     CDS             1828..2790
                     /locus_tag="VN968_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HXL30870.1"
                     /translation="MNFHAAAPTIPPRILCIGMPVRDLTFRIDELPERGFKVNASHFE
                     EICGGNALNAAIGIVRLGGRASICGPMGDARETSAKYIFDNLAHEGIETRHIVHMPGL
                     VTPISNIMIDPSGERTIVTFRDPELWKVRLPDTDELLEDCSAILTENRCAEFCTNLCA
                     EARRRGIPVIVDVDRTMSLREGLLTASSHLVFSSEALQSTAGVADDGEALKKIAKLTP
                     SFLAGTRGALGTLWLDEQQNLQQTPAFPVHTVDTLGAGDVFHGAFALAITEDQDIPSA
                     LRFASAAAALKCTRFGGAFAAPQRAEVSELLSHSPVASPARTHQ"
     gene            2858..3358
                     /locus_tag="VN968_16515"
     CDS             2858..3358
                     /locus_tag="VN968_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXL30871.1"
                     /translation="MTDLAVQLHEPNRRLDAIDRKILTVLQEDASLSVAEIGDRVGLS
                     STPCWKRIQRLEADGVILRRVALVDQNKIGLGISVFVSVESADHSEAWLRKFADAVSA
                     MPEVMEFYRMAGDVDYMLRVVVADMQSYDVFYKKLIGAVPLKNVTSRFAMEKIKSVTA
                     LPIPAA"
     gene            complement(3531..5030)
                     /locus_tag="VN968_16520"
     CDS             complement(3531..5030)
                     /locus_tag="VN968_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HXL30872.1"
                     /translation="MFPKPKSVLVPNTYSYESEPMVKATGFREYDARWLFGKEINLMG
                     IQALGMGLGALIAELGGKQEVVTGHDFRDYSASIKYALISGLQAAGCKVHDIGLCMTP
                     MAYFAQFDLDVPCVAMVTASHNDNGWTGVKMGTNRPLTFGPDEMSRLKEIVLNADFKN
                     KAGGSYQFHEDFPARYIADLTSRPKLKRKLKVVVACGNGTAGAFAPQVMQAIGCEVVP
                     LDTELDYTFPKYNPNPEDMEMLHAIRDAVLEHKADLGLGFDGDGDRCGVVDNSGEEIF
                     ADKVGVMLARDMSAIHKNAQFVVDVKSTGLFVTDPVLQKQGAKTIYWKTGHSYMKRRT
                     HELGALAGFEKSGHFFFNKPFGRGYDDGLISAIAVCEMLDRAPGKSMADLKNALPKTW
                     SSPTMSPHCADEVKYGVVERVLKHFEALQKNGEKVAGQPIRDLVTVNGVRVTVADGGW
                     GLVRASSNKPELVVVVESPVSEQRMRDMFDAMDQVLRSHPEVGEYNQKI"
     gene            complement(5159..5677)
                     /locus_tag="VN968_16525"
     CDS             complement(5159..5677)
                     /locus_tag="VN968_16525"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HXL30873.1"
                     /translation="MRNIVILAAFMVGLGTIMAQMADRMTLAPARANSVSLKVAAVET
                     STQTAGRTLSIPRDARGHFQTDGRIDGQRIGFMVDTGASVIALNESSAARFGLRPSSG
                     DYNATVTTANGKIRAARARLAMVDIGGLVVRDVDAMVLPDEALSENLLGLSFLSRLKR
                     FEYANGKMVLEQ"
     gene            complement(5810..6490)
                     /locus_tag="VN968_16530"
     CDS             complement(5810..6490)
                     /locus_tag="VN968_16530"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXL30874.1"
                     /translation="MSAAYIFDVEGTLIDCAPQTLQCWHETLASFGLSVPMVSLQRLS
                     GMDGSDMLAKLLPSLDESARKKILTAEGERYRAVYLPRVRAFQGVRAVFTAIKALGAR
                     IALATDCQSDELKRYRSLMNVDDMIDAVACGDEVSKGKPEPDLIELALDHLGAIAPGS
                     ATMIGDTPFDAQAARRAGAAAWGTLTGGHPRSSLIGAGCTVVVPAVDDLQRYIERGTP
                     GVWALAPP"
     gene            6627..7529
                     /locus_tag="VN968_16535"
     CDS             6627..7529
                     /locus_tag="VN968_16535"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HXL30875.1"
                     /translation="MGRKIKMAMDETRLLILGSQVLLGFLFQSVFQNGFDSLSPASKN
                     LVCAALALMMLSMGCLIAPSMQHRIVEAGEATSRLGRATTVLAGWALLPFLASLGLGL
                     FIVFDRIFGRVAGVAVGAASAALAGFFWFGLAGLIGEQRRRSMTNQSGSTSLSTKVEQ
                     MLTEARVIIPGCQALLGFQLVAMMTQAFDELPQDAKIMHAAGLCSVTIAMILLMTPAA
                     LHRLSFGGNESERFLRLGSAFVIARTATSRARYCRRCLCGVFQNQPLRGLCPGSKLSA
                     AAGDAGVMVFLSDVAAHIGSQKVR"
     gene            complement(7532..10129)
                     /gene="hrpB"
                     /locus_tag="VN968_16540"
     CDS             complement(7532..10129)
                     /gene="hrpB"
                     /locus_tag="VN968_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HXL30876.1"
                     /translation="MSRSFDTPLPIDAVLDELARTLAGNNAAVVVAPPGAGKTTRVPL
                     ALLDEPWVKNRKIIVLEPRRIAARASAERMAHTLGERVGETVGYRVRFGSKVSRTTRI
                     EVVTEGIFSRQILDDPELAGVATVLFDEFHERSLDADLGLALARDAQTGLREDLRILV
                     MSATLDGARVARLLGDAAVIESEGRAFPVETRYLGRKADAPLERQMADAIAMALRADP
                     GSVLAFLPGTAEIRRTQNFLGERVHDASVEIVPLFGALDASVQDRAIAPAPRGRRKVV
                     LATSIAETSLTIEGVRIVVDSGMARVPRYEPDIGLTRLETVRASRAAVDQRRGRAGRT
                     EPGVCYRLWDEPQTASLAPYTQPEVLSADLSSLVLDLAQWGVSDPSTLAFLDSPPAPA
                     LKEARSLLRELGALDADGRITEEGKSLRALALPPRLARMIVDSHRLGAGEEAADIATV
                     LTERGLGGDSVDLDVRLDQFRRDRSPRASSARALAQRWASQVASSPSPLAGEGRGGGW
                     RATGASVVATPTPNPSPQGGGGRGGSPQDESEHRAELTTGAMLALAFPDRVARNRGNG
                     SFVLANGRGAALDQASALTRAPYIAVAELTGAAASGRILLAAPITQAEIEQYFADQIE
                     TADDISFDRIAMALRARRKKTLHAITLSEAPLALQPSMETAQVLADGLIAAGLNRLPW
                     SKPAKQWRDRVMFLRKAEGEPWPDLSDGALAAQREAWLVPVLSNKTSLKELSSGDLSD
                     ALMTLLPWDLRARLEREAPTHFEAPTGTMLAIDYEAEQGPTIAVRLQELFGLNTHPSI
                     AKGAVPLVLELLSPAHRPVQVTRDLPGFWRGSYAAVRSDLRGRYPRHPWPEDPAGAMP
                     TRRVKPRGT"
     gene            complement(10303..11622)
                     /locus_tag="VN968_16545"
     CDS             complement(10303..11622)
                     /locus_tag="VN968_16545"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HXL30877.1"
                     /translation="MRIAMIGTGYVGLVSGACFADYGHQVTCVDKDGGKVESLRRGEI
                     PIFEPGLEALVASNVKAGRLDFTTDLAGPVAEADAVFIAVGTPSRRGDGHADLSYVYS
                     AAREIAAALTGFTVVVTKSTVPVGTGDEVERLIRETNPSADVVIASNPEFLREGAAIR
                     DFKFPDRIVVGTSDERAKKVLGDIYRPLSLNQAPLMFTARRTAELIKYAANAFLATKI
                     TFINEIADLSEKVGADVQEVARGIGLDNRIGSKFLHAGPGFGGSCFPKDTRALVKIAL
                     DHDVHLRIVEAVLAVNDNRKRAMARKVAGAAGGGLRGKTIAVLGLTFKPDTDDMRESP
                     SIPLVTGLLDMGARVRAHDPVGIEQARRELPDIEYCDDPYLCVRGADAMVVVTEWVQY
                     RTLDLKRLKRELAQPVVVDLRNIYRPEEMAALGFTYESVGRPAERRD"
     gene            complement(11836..>12100)
                     /locus_tag="VN968_16550"
     CDS             complement(11836..>12100)
                     /locus_tag="VN968_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30878.1"
                     /translation="QKVEIELVAFGPGLHMLRDDTSPVKARIKSMSEAMPNLTFAACG
                     NTRENMTKAEAKDIPIISQAKVVNAGVVRLMELQERGWSYLRP"
BASE COUNT         2220 a         3830 c         3769 g         2281 t
ORIGIN      
        1 gggatcgccc atcttgcgcg attcgctcga aaaaacaggt aggagactga cgagcgaccc
       61 gatccggacc tcgttagggc tgcttccttc cggacctgac ccggttggcg agtggctcgt
      121 ccaccgccaa tctcccggtc cctatttggg gccaaaaggt ccggaaagca agctggggcg
      181 gcgatgacct tcaggctgcg agaagagcac caatttcgtc atggccgggc ttgacccggc
      241 catccagtct tgttttccac ccctgagcca aaaagacgtg gatgcccggg tcaagcccgg
      301 gcatgacggg tgttgtttgc ggagccactc tctgcaaggc tattgacgaa ctagagaaac
      361 ccatatgcct tccgacgctc ccgcctggtc gctgcactca cggctcaaag aagacaccat
      421 cgatatcggc gacctgccgc tgtgccgggt gctggtgatc aaggatgcga attacccctg
      481 gttattattg gtgccgcgcc gacatgaggc cgtggaaatc atcgatctgg acgaggtcga
      541 gcaggcgcag ttgatcgcgg agatttcccg cgtcgcacgc gcgctgaaag aaatcaccaa
      601 atgcgacaag ctgaacattg ctgcgctcgg caatctggtg ccgcaactgc acgtgcatgt
      661 catcgcgcgg cgctccagcg atgccgcctg gccgcgcccg gtctgggggg cggtgccgcc
      721 gctcgcgcaa gatgccgagg aagtccagaa tttcatcagc gcgcttcgtc gcaagatctg
      781 gcttggttga actcatgagt gcattcgatt cctttccact ggggcggccg gcctttgtca
      841 gcaatattct cgatcgcgcc gcgcatctgc gcagcgacga tgctaaactc atggcactcg
      901 aagggcgccg cgatgcccgc gcctatgtgg tttatcgcga ctcgctggtg ctgaagcagg
      961 aggccgacgg tccgcgcgcg ctcttgagca tcgacgaagc gctgaaattc ggcgccaatc
     1021 cgggcacgat cttcctgggc ttgcgcgacg gcgcggcggt gttcggcatg ggcatcccgg
     1081 cagcggcggt ggaaaaactg ctggcgcgta gtgatgtcgc cgtcaccgag ctgcgcggca
     1141 tggcgatgca gggcgtggtg ccgcccgacc aactctcggc gatcgcgatg gcgaaatcga
     1201 tggtgagctg gcatcagcgt cacggctact gcgccaattg cggaacccgc accgcgatga
     1261 aggacggcgg ctggaagcgc gaatgcccca actgcaaggc cgagcatttt ccgcgtaccg
     1321 atcctgtcgt aatcatgctg gtgacctcgg gcgacaaatg cctgctcggg cggcagaagc
     1381 aatttccacc cggcatgtgg tcgtgtcttg cggggttcgt cgaagccgcg gaaaccatcg
     1441 aggatgcggt ccggcgcgag atcctcgagg aatccggcat tcgctgcacg gatgtgaatt
     1501 attacatgac gcagccctgg ccctatccgt cgtcgctgat gatcggatgc accgcgcgcg
     1561 ccaccaacga ggatatcgtg gtcgaccgcg cggaactgga agacgcgcgc tggttcgacc
     1621 gggccgaggc cgcgctgatg ctgaaacggc aacatccgaa tggccttgcg gggcctcacc
     1681 cgttcgcgat tgcccatcat ttagtcggcc gctggctgca gacagaccag cgtccaggcg
     1741 catagctgga acccggctca tggcctatcg gttaacggca agtgtcagct tccgtcgcgg
     1801 ggcaggacgg atgtgatgga cgagccgatg aactttcacg ctgccgcgcc gacaatcccg
     1861 ccgcgcatcc tgtgcatcgg catgccggtg cgggatttga cctttcgcat cgacgaactg
     1921 ccggagcgcg gcttcaaggt caacgccagc catttcgagg aaatctgcgg cggcaacgcg
     1981 ctcaatgcgg cgatcggaat cgtgcgcctc ggcggccgcg cctcgatctg cgggccgatg
     2041 ggcgatgccc gtgagacttc agccaagtat attttcgaca atctcgcgca tgaaggcatc
     2101 gagaccaggc acatcgtgca catgcccggc ctggtgacgc cgatttcgaa catcatgatc
     2161 gacccctcgg gcgagcgcac catcgtgacc ttccgcgatc cggaattgtg gaaggtccgt
     2221 ttgccggaca ccgacgaact gctcgaggac tgcagcgcca tcctgaccga gaaccgctgc
     2281 gcggaatttt gcaccaatct ttgcgccgag gcacgcaggc gcggcatccc tgtcatcgtc
     2341 gatgtcgacc gcacgatgtc gctgcgcgaa gggctgctca ccgcttcgtc gcatctggtg
     2401 ttctccagcg aagcgctgca gtcgaccgcc ggcgtcgccg atgacggcga ggcgctcaag
     2461 aagatcgcaa agctgacccc ctcatttctg gcgggaacgc gcggtgcgtt gggcaccctc
     2521 tggctcgacg agcagcagaa tctgcaacag accccggcct tcccggtcca tacggtggat
     2581 acgctgggcg ccggcgacgt attccatggc gcgtttgcac tcgccatcac cgaagatcag
     2641 gacataccga gcgcgctgcg gttcgcctcc gccgccgccg cactcaaatg cacccgcttc
     2701 gggggtgcct ttgccgcgcc gcaacgtgct gaagtctcag aacttttgag ccacagcccg
     2761 gtggccagcc cggcccggac gcatcaataa tgggcttgtc ctagaagaat tttctatata
     2821 tgagggagat cagcctcgta tatggaacaa agttctcatg accgatctcg ccgttcagct
     2881 tcacgagccc aaccgccgcc tcgatgccat cgaccgcaag attctgacgg tgctgcagga
     2941 agatgcctcg ctctcggtcg ccgagatcgg cgaccgggtg ggactttcct cgacgccgtg
     3001 ctggaagcga atccagcgac tggaagccga cggggtcatc ctgcgccggg tcgcgctggt
     3061 cgaccagaac aagatcgggc tcggcatttc ggtctttgtg tcggtcgaaa gcgccgacca
     3121 ttccgaggcc tggcttcgaa aattcgccga cgccgtcagc gcgatgcccg aggtgatgga
     3181 gttttatcgc atggccggcg acgtcgatta catgctgcgc gtggtggtcg cggacatgca
     3241 gagctacgac gtgttctaca agaagctgat cggcgcggtg ccgctaaaaa atgtcacctc
     3301 gcgctttgcg atggagaaga tcaaatccgt caccgcgttg ccgatcccgg cagcgtaatc
     3361 caaaggcgac gctgaatagc tccctcgccc cgctcttgcg gggagagggt tggggtgagg
     3421 ggccgcttcc gcaagtttct catgatgaag tgcgcgcggt gagtccccct cacccggatc
     3481 gctaaaggcg atccgacctc tccccgcaag cggggagagg tgaagaaaaa tcagatcttc
     3541 tgattgtatt cgccgacctc gggatggctg cgcagcacct gatccatcgc atcgaacatg
     3601 tcgcgcatgc gctgttcgct gaccgggctt tcgaccacca ccaccagctc cggcttgttt
     3661 gacgatgccc gcactaggcc ccagccgccg tctgcaacgg tgacgcgcac gccgttgacg
     3721 gtgacgagat cgcggatcgg ttgccccgca accttctcgc cgttcttctg cagcgcctcg
     3781 aaatgcttca gcacgcgctc gacgacgccg tatttgacct cgtcggcgca atgcggcgac
     3841 atcgtcggcg acgaccaggt tttcggcagc gcgttcttca gatctgccat cgacttgccg
     3901 ggggcgcgat caagcatctc gcagaccgcg atcgccgaaa ttaagccgtc gtcatagccg
     3961 cggccgaacg gcttgttgaa aaagaagtgg ccggatttct cgaaccccgc cagcgcgccc
     4021 aattcgtggg tgcgacgctt catataggaa tggccggtct tccagtagat ggttttcgct
     4081 ccctgctttt gcagcaccgg atccgtgacg aacagcccgg tcgatttgac gtcgacgacg
     4141 aactgcgcgt ttttgtggat ggcagacatg tcgcgcgcga gcatgacgcc gaccttgtcg
     4201 gcgaaaatct cctccccgct gttgtcaacc acgccacagc ggtcgccgtc gccatcgaag
     4261 cccaggccga gatcggcctt gtgctcaagc accgcgtcgc ggatggcgtg cagcatctcc
     4321 atgtcttcgg gattgggatt gtatttcggg aaggtgtagt cgagttcggt gtcgagcggc
     4381 acgacctcgc atccgatcgc ctgcatcacc tgcggcgcga atgcacccgc ggtgccgttg
     4441 ccgcaggcga ccacgacctt cagcttgcgt ttcagttttg ggcggctggt cagatcggcg
     4501 atgtagcgcg ccggaaaatc ctcgtggaat tgatacgaac cgccggcctt gttcttgaag
     4561 tcggcattga gcacgatttc cttcaggcgg ctcatttcgt cgggaccaaa agtaagaggc
     4621 cggttggtgc ccatcttgac cccggtccag ccattgtcgt tatgcgacgc cgtcaccatc
     4681 gcgacgcagg gcacgtcgag atcgaattgc gcgaaatacg ccatcggggt catgcacagg
     4741 ccgatgtcgt gaaccttgca gcccgcggcc tgcagccctg aaatcagcgc gtatttgatc
     4801 gaggccgaat aatcacgaaa gtcgtgcccg gtgacgacct cctgtttgcc gccgagttct
     4861 gcgatcagcg cacccaaccc catgccaagg gcctgaatgc ccatcaggtt gatttccttg
     4921 ccgaacagcc agcgggcgtc gtattcgcga aagccggtcg ccttcaccat cggttcggat
     4981 tcgtaggaat aggtattcgg aaccagcacg gatttcggct tcggaaacat ttatgacggc
     5041 ctcggcgaga aacgctgaaa ggaaagcact gcaggcatag ccaatgcgca ggcgcagcga
     5101 aaggcgggac agcggttttt gggcgatgaa ttgcggccga ttggcagcca agcgagcctt
     5161 actgctccag aaccatcttg ccgttggcgt attcgaagcg cttcagcctc gacaggaacg
     5221 acaggccgag caggttctcc gacagcgctt catccggcag caccatggca tcgacgtcgc
     5281 gcaccaccag accgccgata tcgaccatcg cgagccgcgc gcgggccgcc ctgatcttgc
     5341 cgttggcggt cgtcaccgtg gcgttgtagt cgccgctgga gggacgcagg ccaaaccgcg
     5401 ccgccgagct ttcgttcagc gcgatcaccg atgcgccggt atcgaccatg aagccgatgc
     5461 gctgaccgtc gatgcgccca tcggtctgga aatggccgcg ggcatcgcgg gggatgctga
     5521 gggtgcggcc agcggtctgc gtcgatgtct cgacggcggc gactttcaga gagacggaat
     5581 ttgcccgcgc cggcgccagc gtcatcctgt ccgccatctg tgccatgatg gtgccaagac
     5641 cgaccatgaa cgcggcaaga atcacgatgt tacgcatcac gccaaactcg atcagaggag
     5701 ccgcaatatc accacgccag ccccaaaacc gcgaccgatg gcggcgggga aggtggccat
     5761 tttggcgaaa aggatgaccg aagtgttaac gggcgccggt tggcggctgt tacgggggcg
     5821 ccaaagccca gacacccggc gttccccgct cgatatatcg ctgcaaatcg tcaaccgcag
     5881 gcacaacgac ggtacagccg gcaccgatca gcgatgacct gggatggcct cccgtgagcg
     5941 ttccccatgc ggcagcgccg gcacgccgcg cggcctgagc atcgaacggg gtatccccga
     6001 tcatggtggc tgaccccggc gctatggcac cgagatgatc gagcgccagc tcaatcaagt
     6061 ccggctcggg ctttcctttt gagacctcat cgccacaggc gacggcgtcg atcatatcgt
     6121 cgacattcat aaggctgcga taacgtttca attcgtcgct ttgacaatcg gtggcgagtg
     6181 cgatgcgggc accgagcgct ttgatcgcgg tgaacaccgc gcgcacgcct tgaaacgccc
     6241 gcacccgcgg tagatagacc gcgcgatagc gctcgccttc cgccgtcagg attttctttc
     6301 gtgcgctttc gtcgaggctg ggcaggagct ttgccagcat gtcgctgcca tccatcccgg
     6361 acaatcgctg caacgaaacc atcggcaccg acaggccaaa gctggcgagc gtctcatgcc
     6421 agcattgcag ggtttgcggc gcgcaatcga tcaaggtgcc ttcgacgtcg aagatgtagg
     6481 cggcgctcaa tccacactcc aacaatggtt ccggaaggga acgcgccgcc catcaaagag
     6541 ttcactaccc gacatgtttt ttgtctccca attcatcggc gattggagct ggcgttcgtc
     6601 tcgcagaagg atgacccttt ttggatttgg gccgaaaaat aaaaatggcg atggatgaaa
     6661 cgcggctgct gattctggga tcgcaggtgc tgttgggatt tctgtttcaa agcgtgttcc
     6721 agaatggctt cgattcgctt tcgccggcct cgaaaaatct ggtctgcgcc gcactggcat
     6781 tgatgatgct gtcgatggga tgcctgattg cgccatcgat gcagcatcgt atcgtcgagg
     6841 ccggtgaggc gacctcgcgt cttgggcgcg caacgactgt ccttgccgga tgggccttgt
     6901 tgccgttcct ggcgagcctc ggccttgggc tgtttatcgt gttcgaccgc atctttggca
     6961 gggtcgccgg cgtcgcggtc ggtgccgcga gcgccgcatt ggcaggtttc ttctggttcg
     7021 gcctcgcggg gctgatcgga gaacaaagga gacggagcat gaccaatcaa tccggctcga
     7081 cgtcgctctc gacaaaagtc gagcaaatgc tgaccgaggc ccgggtcatt attcccggct
     7141 gtcaggcact gttgggtttt caactcgttg cgatgatgac ccaggcattc gacgaattgc
     7201 cgcaggacgc caagatcatg cacgccgccg gattgtgctc tgtcacgatc gcaatgatcc
     7261 tgctgatgac gcccgcggcg ttgcatcggc tgtcattcgg cggcaacgaa tcagagcggt
     7321 tcctgcgtct cggttcagcc tttgtcatcg cccggaccgc tacctctcgc gctcggtatt
     7381 gccgccgatg tctatgtggt gtttttcaaa atcagccgct ccgcggcctt tgccctggta
     7441 gcaagctcag tgctgctgct ggcgatgctg gcgttatggt atttttatcc gatgtggctg
     7501 cgcacatcgg gagtcaaaaa gtgaggtgac gtcacgtgcc ccgcggcttg acgcgccggg
     7561 ttggcatcgc gcccgccgga tcctccggcc aggggtgccg cggatagcgt ccgcgcagat
     7621 cggagcggac cgccgcataa ctgccgcgcc aaaaacccgg caggtcgcgc gtcacctgta
     7681 ccggccggtg cgctggcgac agtagctcca gcaccagcgg caccgcgcct ttggcgatcg
     7741 acggatgggt gttgagcccg aacaattcct gcaagcggac agcaatcgtc ggcccctgct
     7801 cggcctcgta gtcgattgcg agcatggtgc cggtcggcgc ctcgaaatgc gtcggtgctt
     7861 cgcgctccag ccgcgcccgc aagtcccagg gcagtagcgt catcagcgca tccgacaggt
     7921 cgccggacga caattccttc aacgacgtct tgttggagag caccggcacc agccaggcct
     7981 cgcgttgtgc cgcgagcgcg ccgtcggaca gatcgggcca cggctctcct tccgccttcc
     8041 gcaagaacat cacgcggtcg cgccactgtt ttgctggctt cgaccatggc agccggttga
     8101 ggccggcggc gatcagacca tcggccaaaa cctgcgctgt ctccatcgag ggctgcagcg
     8161 ccaacggcgc ttccgaaagc gtgatggcgt gcaatgtttt cttgcgccgc gcccgcaagg
     8221 ccattgcaat gcgatcgaag gaaatgtcgt cggcagtctc gatctgatcg gcaaaatact
     8281 gctcgatctc tgcttgcgta attggcgccg ccagcaggat gcgtccgctg gcagcggcgc
     8341 cggtcaactc ggcgaccgcg atgtaaggcg cgcgcgttag tgcggatgcc tggtccagcg
     8401 cggcgccgcg cccgttcgcg agcacgaagc tgccattgcc gcggttgcgc gcgacacgat
     8461 cgggaaatgc caacgcaagc atcgcgccgg tcgtcagctc cgctcgatgc tcgctttcat
     8521 cttgcgggga ccctccccgc ccgccgcccc cttgcgggga ggggttaggg gtgggggtag
     8581 ccacaacgga ggcgccggtg gctctccacc cccctccccg accctccccc gcaagggggg
     8641 agggagaaga agccacctgc gaggcccagc gctgcgcgag agcgcgcgcg ctgctggcgc
     8701 gcggcgagcg atcgcggcgg aattggtcga gcctgacgtc gaggtcgacg ctgtcgccgc
     8761 cgagcccgcg ctcggtgagc acggtggcaa tatccgcggc ttcctcgccc gcgcccaggc
     8821 gatgggaatc cacgatcatc cgcgccagcc gcggcggcaa cgccagggcg cgcaggctct
     8881 tgccctcttc ggtgatccgg ccatcggcat cgagtgcccc gagttcgcgc aacaggcttc
     8941 gcgcctcctt cagcgcgggc gccggcggcg aatcgagaaa cgccagcgtc gagggatcgc
     9001 tgacccccca ttgcgcgagg tcgagcacca gcgaagacag atcggcgctg aggacttccg
     9061 gctgggtata gggcgcaagc gacgcggtct gcggctcgtc ccagagccgg tagcacacgc
     9121 caggttcggt acggccggca cggccgcggc gctgatcgac cgcagcgcgc gaggcgcgca
     9181 cggtctccag ccgcgtcagc ccgatgtccg gctcatagcg cggcacccgc gccatcccgg
     9241 aatcgacgac aatgcggacc ccttcgatgg tcaatgaggt ttccgcgatc gacgtcgcca
     9301 gcacgacctt gcggcggccc ctcggcgcgg gtgcgatggc gcgatcctgc acactggcgt
     9361 cgagcgcgcc gaacagcggc acaatttcga cagaggcgtc gtggacccgc tcgccgagaa
     9421 aattctgggt gcggcgaatt tcggctgtac ccggcaggaa cgccagcacc gagcccggat
     9481 cggcgcgcag cgccatcgcg atggcatccg ccatctgccg ctccagcggc gcgtcggcct
     9541 tgcggccgag atagcgcgtc tcgaccggga acgcgcggcc ctcgctctcg atcactgccg
     9601 cgtcgccgag cagtcttgcc acccgcgcgc cgtcgagcgt cgccgacatc acgaggatgc
     9661 gcaggtcctc gcgcaagccg gtttgcgcgt cgcgcgccag cgccagcccg agatcggcat
     9721 cgagcgagcg ctcgtggaat tcgtcgaaca gcaccgtggc gacgcctgcc aattccggat
     9781 cgtcaagaat ctgccgcgaa aaaataccct cggtgaccac ttcgattctt gtcgtgcgcg
     9841 acacttttga gccgaaccgt acccggtaac cgacagtctc gccgacgcgt tctccaaggg
     9901 tatgcgccat ccgctcggcg ctggcgcgtg ccgcgatccg ccgcggctcc agcacgatga
     9961 ttttcctgtt tttcacccag ggctcgtcga gcagcgcgag cggcacgcgc gtggtcttgc
    10021 cggcgccggg cggcgccacc acgacagctg cattgttgcc ggcaagcgtc cgcgccaact
    10081 cgtcgagaac cgcgtcgatc gggagagggg tgtcgaagct gcgggacaaa gtttcaccaa
    10141 ttcgtcatgc ccgggcatag tagtctgccc ggcgcagact acgtaaactt gtccgcgctc
    10201 ccgggcatcc acgtcattct tgggcgagga aacaagacgt ggatggccgt gacaagcacg
    10261 gccatgacgg aaattgtcgc ggtgcgcagc tcaaagagtc cgtcaatccc gccgctcagc
    10321 cggccgcccg acactttcat aggtaaagcc gagcgccgcc atttcctcgg gacgatagat
    10381 gttgcggaga tcaacgacca ccggctgcgc cagctcgcgc ttcaggcgtt tcagatcgag
    10441 ggtgcgatat tgcacccact ccgtcaccac caccatcgca tccgcgccgc gcacgcagag
    10501 gtagggatcg tcgcaatatt cgatgtcggg caattcgcgg cgcgcctgct cgatgccgac
    10561 cggatcgtgc gcgcgcaccc tggcgcccat gtcgagcagc ccggtcacga gcgggatcga
    10621 cggcgactcg cgcatgtcgt cggtatcggg cttgaaggta aggccgagca cggcgatggt
    10681 cttgccgcgc aggccgccgc cggccgcgcc tgcgaccttg cgcgccatcg cccgcttgcg
    10741 gttgtcgttg accgcgagca ccgcctcgac gatccgcaag tgaacgtcat gatcgagcgc
    10801 gatcttgacc agcgcgcggg tgtcctttgg aaagcacgag ccgccgaagc ccggcccggc
    10861 atgcaggaat ttcgaaccga tccggttgtc gagcccgatg ccgcgtgcga cctcctggac
    10921 gtcggcgccg accttttcgg agagatcggc gatttcattg atgaaggtga tcttggtggc
    10981 gaggaaggcg ttggccgcgt atttgatcag ctccgcggtg cgccgcgccg tgaacatcag
    11041 cggcgcctga ttgagcgaca gcggccgata gatatcgccg agcacttttt ttgcgcgttc
    11101 atccgacgta ccgaccacga tgcggtccgg aaacttgaaa tcgcggatcg cggcgccctc
    11161 gcgcagaaat tccggattgg acgcgatcac gacatcggcg gaaggattgg tttcccggat
    11221 caggcgctcg acttcgtcgc cggtgccgac cggcacggtc gatttggtga cgaccacggt
    11281 gaagccggta agcgctgctg caatctcgcg cgcggcgctg tagacgtagc tgaggtcggc
    11341 gtggccgtcg ccgcgccgcg acggtgtgcc gaccgcgatg aacaccgcat cggcctcggc
    11401 gaccggcccg gcgagatcgg tggtaaaatc cagccgcccg gcctttacgt tggaagccac
    11461 cagcgcttcg agcccaggct cgaaaatcgg gatttcaccc cggcgcaagc tttcgacctt
    11521 gccgccatcc ttgtcgacgc aggtgacctg gtggccgtag tccgcaaaac aggcgccgga
    11581 caccagcccg acatagcctg tgccaatcat ggcaatccgc atgatctttc ttactctcca
    11641 tatgtggatt tgacgaactt cacaaagtgc atcgaaattt accagcgcaa aactactgct
    11701 gtcggtgaat tagcttgcta agctaaatag gtattcgcga gggcgcactt tgaaccaaca
    11761 gtggctcttg gtgacgtgga gtcaaaaact agtctcgaag tctcaccgtt cagagccgga
    11821 agccgtgtga agtcatcacg gtcgcaggta actccagcct cgctcttgca gttccatgag
    11881 ccggacgact ccggcattta cgaccttcgc ctgcgagatg atagggatat ctttcgcctc
    11941 agcttttgtc atattttcgc gagtattccc acatgcagca aaggtgaggt ttggcatggc
    12001 ttcgctcatg gacttgatgc gcgctttgac gggtgaggtg tcatctcgca gcatgtgcag
    12061 tccgggtcca aaggcgacca gttcgatctc aaccttttgg
//
LOCUS       DATNOI010000413        18402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1819029,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000413 DATNOI010000000
VERSION     DATNOI010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18402
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819029"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VN968_16555"
     CDS             complement(<1..368)
                     /locus_tag="VN968_16555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30879.1"
                     /translation="MPPSADRMPPMWRRLVLAVAALIAAGYGLTLTIFYPGILTYDAK
                     FVYEDIAKGVLGDWQSPVMTVLWGVIDPVAPGAGSMFLLIATSYWLGFGLLAFALARR
                     STRLALVLPLLALMPPAFVFV"
     gene            complement(352..1098)
                     /locus_tag="VN968_16560"
     CDS             complement(352..1098)
                     /locus_tag="VN968_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXL30880.1"
                     /translation="MFEGFRTARGIFRSLRIYYGDRSRAAAMDRLYGGLVRHGDLVFD
                     IGAHVGDRVASFRRLGARIVAVEPQPAMLKVLKLFYGRSADVAIEPVAVGRSLGATSL
                     MINADNPTVSTASREFVTAARDAQGWETQRWIKSMPVPVTTLDALIGKYGAPAFIKID
                     VEGFEEEALQGLSVAVKALSFEFTIIQRHVALACIERCIALGYTRFNAALGESQVFVN
                     ADWVGGEEIARWLTGLPHAANSGDIYAALG"
     gene            complement(1103..2905)
                     /gene="mdoH"
                     /locus_tag="VN968_16565"
     CDS             complement(1103..2905)
                     /gene="mdoH"
                     /locus_tag="VN968_16565"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003962.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HXL30881.1"
                     /translation="MLERSPPQVMQADATENDIFGRRVLFAGLFATTMVGSLALAALA
                     LAPGGFGVIDMTLLALFAVTLPWMVAGFWNAVIGFLIMRFSADPIAAVMPAAARIRGD
                     EPVTASTAILLCTRNELPERMIRNLEPMLAGLDAAGCGERFHLYVLSDTSDAAIADHE
                     EACFASLIARWRDRVAITYRRRAVNTGYKAGNVREFCERWGRQHDFAVTLDADSLMTA
                     DAVLRLVRIMQADPRLGILQGLIVGLPSTSAFARVFQFGMRLGMRSYTIGSAWWQGDC
                     GPYWGHNAALRLEPFIAHCSLPVASDGTEDRHILSHDQIEAVLMRRAGYDVRVLPQED
                     LGWEENPPTLLEFVRRDLRWCQGNMQYWRFLSLAGLKPVSRYQLVLAILMFIGSPAWI
                     GLLVIGTLAVAFSDAPQNFIRADAGMALFVCVLVMWFSPKIASAVDILMRPELRRGFG
                     GAGLFIVSYAVETVYSILLCPILWFGHTIFLTGLLFGREIGWIGQTRDDHAVPFTLAL
                     RNLWPHTLLGCGALGLLAVTQPAAVPYALFLAGGPALAIPIAMMTAWPRLGHFAARVG
                     IGRLPEETTRPVVLLALALPAIEQTAPLPRPSSV"
     gene            2995..3984
                     /locus_tag="VN968_16570"
     CDS             2995..3984
                     /locus_tag="VN968_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HXL30882.1"
                     /translation="MAISRPEQVAQALWYSGPGQADIRQETLAPPGAGEVRVRALFGA
                     ISRGTEALVLAGRVPESEFERMRAPFMAGHFPFPVKYGYATVGQIEGGTEALLGRTVF
                     TLHPHQDVFNVPASAAVVLPKNLPPQRAVLAANMETALNAVWDAAPGPADRIAIVGAG
                     VVGSLVAYLCGRIPGTEVTLVDINPARAELAKTLGVSFAKPETAKGDCDLVVHSSGHP
                     AGLGTALALAGEEATVLELSWYGDASVAAPLGGGFHSRRLRLVSSQVGRIAPSHRPRW
                     THGRRLAAALDLLADARLDALLAPAVVFEDLPRRLVDILDASRGVLCQLIAYP"
     gene            3995..4396
                     /locus_tag="VN968_16575"
     CDS             3995..4396
                     /locus_tag="VN968_16575"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HXL30883.1"
                     /translation="MFAVEVRDHIMIAHSFQGAVFGPAQALHGATFVIDAAFLADTLD
                     PNGIVIDIGRAHDALKAIVATLNYRNLDDVPEFKGHNTTTEFLTRHIFDRLAEEARAG
                     ALGRDGRELKAIRVTLSESHVARAWYEADLW"
     gene            4393..5442
                     /locus_tag="VN968_16580"
     CDS             4393..5442
                     /locus_tag="VN968_16580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXL30884.1"
                     /translation="MKRFAFAVPGDLATPTGGYAYDRRMMTELCDLGWQIDLLDLGEG
                     FPRPDEATRATAQSRLLAMPAGRCIVVDGLALGVLPETASQLAGRNPLLALVHHPLAL
                     EWGLSVKQADTLRASEQAALAAVQGVVVTSAATAKLVASDYGVPAERIAVARPGSDPA
                     LMSQGSQDGVVRLLSVGAIVPRKGFDVLIAALATLTDLSWRLTIAGDRTRDRNAAARL
                     DAGIARHALGDRIAALGAVSPQRLAALYAEADVFVLASHFEGYGMAYAEAVAHGLPVI
                     GTSAGAIPDTVPPDAGLLVDPGDSFPLARALRRIIGDADLRRRLASAARAAAPQLPTW
                     RHSAEIFARALETLA"
     gene            5439..6248
                     /locus_tag="VN968_16585"
     CDS             5439..6248
                     /locus_tag="VN968_16585"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXL30885.1"
                     /translation="MSGFSADWLTLREPYDLRARNPSVLDAVAASLEPLSSVRVVDLA
                     CGTGSTLRALSPHLPARQNWKLIDNDLGLLARAEATPLAKHATLAAIPLDLNRDLEAA
                     LDGPVDLVATSALLDLVSETWLDRLAVEIAARSIPLYAALSYDGRIGFTPPDPRDAAI
                     VAAVNAHQRTDKGFGPALGPAAAAFAIARFEALGYSVVHGASAWTMGPDDLDIQIEIL
                     SGWASSAHEMGALSLADTTAWLARRGDAVAAGCSTLHVGHVDFFATQSATR"
     gene            complement(6209..7018)
                     /locus_tag="VN968_16590"
     CDS             complement(6209..7018)
                     /locus_tag="VN968_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HXL30886.1"
                     /translation="MPISNFPSSKSVSSDPWEDFSDIFRRERQPLPHAWADVFGPLRK
                     GTVDDFVIVGQIGQSLDGRVATESGHSKYINGPAGLVHLHRLRALVDAVVVGVGTAVA
                     DDPQLTVRRVSGPQPARVVIDPKGRLGADARMFADNGVRRLLITEQGTRCTAPSGVEV
                     VTLPVADGQFAPSAILTALGRCGMRRVLIEGGADTLSRFLVAGCLDRLHVIVAPIILG
                     TGGPGLILPSLDRADQAPRMPVRVHKIEDDVLFDCDLSAQRVALCVAKKST"
     gene            7191..8321
                     /locus_tag="VN968_16595"
     CDS             7191..8321
                     /locus_tag="VN968_16595"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HXL30887.1"
                     /translation="MNEAQTGASSALFGSRSHISVSRRLGEFHGRRPVLFTATGEAVL
                     ALPVEGLDRRRLLEFMALCAPVMPQLIITERRALALGLDASTPTALTLSAGDNANTIL
                     ALVTDATNHRMPAATPASPAAAAAIQLAKLSQSLPAVLAADVVARAVACEESIVRVEA
                     DSVASFADDAIRSLIVASEASVPLNSGTPARFVVFRDAMGGSQVAIIVGKPDFTNAVP
                     VRLHSACLTGDVFGSRRCDGGDQLRLAVALLQDLGGGVILYLAQEGRGLGLTNKMRTY
                     QLQDDGLDTFDANTTLGFDDDERDYGIAAHMLRMLNCTRIVLLTNNPAKLDGLTRAGI
                     EIAGRMPLDAPVNADNRRYMTAKATRAGPQFDHLTASLADQA"
     gene            8385..9794
                     /locus_tag="VN968_16600"
     CDS             8385..9794
                     /locus_tag="VN968_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30888.1"
                     /translation="MLRALVVGTGLCWSVAFVALALCYELELYADGAMFSYAVAVQDV
                     WAFHWHNISGRLSVFVLSLLPAEIYVGLSGNPGAGIVVYGLLFYLAPLAGLIGTFAAD
                     RSHGRIIFVYACCSTALLCPLIFGFPTEMCLAHAVFWPALAVSHYAKPTVAGTALVFV
                     MLLTLAFTHEGALVLAFAIVATLAPRGLRDALFLRAAAVLAVVLVMSAAVKIMIPPDE
                     YYAGVLVRAALNFFDPAIFQVSIVLLLFATLAGYAGINLMLSRLAPNRAYLHAAAIMI
                     AVLAVYWLWLDHTIHASSRYYLRTALVVVTPVFGALAALGAMSGDRRLAFPFSSLQQA
                     MTALQRRAARPLAVAFMLSTLVHVVETGKFVAAWTDYRAAVTSLATGDQSDPALGDPR
                     FVSSERIASRLNRLAWFSTTPYLSVIVANFMPGRLVIDPTGNYFWLSCATATANDKAA
                     RAVPEEGRDLLRIYSCLHR"
     gene            9934..10233
                     /locus_tag="VN968_16605"
     CDS             9934..10233
                     /locus_tag="VN968_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30889.1"
                     /translation="MAEADLDVVIRQLAKQQNKGLMAAAKSRRNRYIGLAAKAKNKEA
                     KERYKQIAKNIMLQGTAAAKRLQISADNAADSYARSMRQAAEAQATQKAVKKTKT"
     gene            complement(10346..10960)
                     /locus_tag="VN968_16610"
     CDS             complement(10346..10960)
                     /locus_tag="VN968_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30890.1"
                     /translation="MCILSGRLGLALVLAALCLGGRTAHAQAAPVNYWIPAWPVGFGG
                     NPTVGQNSNAYGNFPSFDGSDARGGGFSYMRYNFPNGWFVGGEGGGMGLSMNGINRDG
                     AFGNIGSLYYFGYNFQNAPLTIYAGFDTLKYNAGIGSPFAPFDSTSGTLPGYSAHAGV
                     EFQPTPNVSLSLGFGYTQQSGRIDSDINSLSLPGASPFAASGRR"
     gene            complement(11167..11502)
                     /locus_tag="VN968_16615"
     CDS             complement(11167..11502)
                     /locus_tag="VN968_16615"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXL30891.1"
                     /translation="MRGLVTVVVAWIFIGDVAYSEENVPDLKDPKMISSGYDVFLEKQ
                     CAHCHGVDGRGGINLARRDLDPKGVFQSIADGREKSGLRMPAWREVLTDEEIWKATAY
                     VMSISQRSK"
     gene            complement(11586..13250)
                     /locus_tag="VN968_16620"
     CDS             complement(11586..13250)
                     /locus_tag="VN968_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXL30892.1"
                     /translation="MAKTTLAASFGLACLLCTAGVALAADPGTEDPNNWPQYHRTTNA
                     WRYSPLDQINKDNVTKLSVAWIAHGGDITMGIQETPLAIDGVIYSITSGNRVAALDGK
                     SGQELWSFQPKLDPLTKKVLFSPYSRGVAVGHGMVFIGTVDGRGIALDQKTGKEKWQV
                     QLTDFANCHGCNFTSPPVVAGDILTFGSTAGELATQGKIFGVEAETGKKVWEFNTIKN
                     DQKSWPGESGKYGGGGAWMPGTYDAETNTIFYGTGNPGKDFVNSDRQGDNLYTDSVVA
                     LDAKTGNLKWYRQEIKQDVWDYDSPYEVMLFKKNGKDVIVHMNKSGYVFVLEKNSGNI
                     ENIWPVSDIKNFVKDIDRENGELIGRVELAMNQETLICPSTFGGRSWNSGAYNPKTGL
                     WYNNVLDFCGYLKPVEQKEDPKDYGTGHTGSNDFGRLAMAPDGRKPGRLDARDPFTGE
                     RKWTYEMDIPGFASVLTTGGGLVFNGDPMGILRAFDADTGKVLWSFNTGSGMRSGIVS
                     YAVDGEQYILVPSGWGSYAAVLLPALFPQLEKVPAASALIAFKLAR"
     gene            complement(13876..14073)
                     /locus_tag="VN968_16625"
     CDS             complement(13876..14073)
                     /locus_tag="VN968_16625"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HXL30893.1"
                     /translation="MDPRLSVVFRQCARQVAKLLRGAKVLDVMVEQPTGFELIINLKT
                     AKTLGVEIPPTLLSRADTAIE"
     gene            complement(14804..14947)
                     /locus_tag="VN968_16630"
     CDS             complement(14804..14947)
                     /locus_tag="VN968_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30894.1"
                     /translation="MAVILALALIAIAFEIYSDTALTSAGVSHTAAPEPVTAAVAHAL
                     RRG"
     gene            complement(15086..15421)
                     /locus_tag="VN968_16635"
     CDS             complement(15086..15421)
                     /locus_tag="VN968_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXL30895.1"
                     /translation="MKLSTLALALTGLAGLIIGICGSAPSEADAKPVAYKLPNQTATF
                     KPGPNLEVVQNNCTACHSVDYVQTQPRGPKFKEIFWRAEVTKMIKVYGAPIDEADVGK
                     IVDYLTQTY"
     gene            complement(15432..16643)
                     /locus_tag="VN968_16640"
     CDS             complement(15432..16643)
                     /locus_tag="VN968_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXL30896.1"
                     /translation="MIDRRSFIKHSGAAIAALGAGMISPSLRAFAATAALPFDNGERP
                     LVKYPQKRPMIGLTSRPPQLETPFSAFNANVITPNDAFFVRYHLADVPLNIDPDAFSV
                     GIQGKVDKPIKLSLADIKKMPAVDIVAVNQCSGNSRGFFNPRVAGGQLGNGAMGNARW
                     RGVALKAVLDRAGVQPGARQVTFNGMDGPVTNKTPDFVKALDIDHARDGEVMLAYSMN
                     GADLPVLNGFPLRLVVPGYFGTYWVKHLNEITVIDNVFDGFWMKTAYRIPDNDCNCVE
                     PGTAPKATVPINRFKVRSFITSVADGAKLRAGAQTTLKGIAFDGGKGIKDVAVSTDGG
                     KTWAQASLGRNLGKYSFREWRMPVRLAAGAHELKVRATSNGGETQPMEPLWNGAGYLR
                     NVVETVRVTAA"
     gene            complement(16816..18216)
                     /locus_tag="VN968_16645"
     CDS             complement(16816..18216)
                     /locus_tag="VN968_16645"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXL30897.1"
                     /translation="MTRFKATFTADGCSPQTEQTKSNRISDAALTSRHALNGPQITAK
                     GKVMGVFTATARNLFVCLSFFLFLISLTVPLRPVLAQSQKPVAQSQKPAVSYDEKKRQ
                     ANDIAVTVVVSGISCTCARFTEDIRNVVNDLGPDGVRVLPVLGVGGLQNVNDVLFLKN
                     MDMAVVDEDNLRLLKKKDPVLYANVEQRVQYITKLYNSEFHVLARNEIKSYDDLKGRK
                     VNFNLKDSQTEVTADTVFSVLNIPVQRSYYDNDEALKRLMSGEISAMIILTGAPQALL
                     AKVKKDDGVHLLPLDQESLQNHDLRDLFASYLPAEMTHENYPNLIAEGTTVPTIANRA
                     LLVAYTWPENSPRYKKVAKFIDAFFSKIDQFNNPSRHPKWREVNLSAEMPGWVRFKPA
                     AEWLAAHRNQAVSANPDGTLGPSSPELRLAFEKFMQNYAASSGRKTLSTKEREMLFAR
                     FMKILAESKVEQAAAH"
BASE COUNT         3458 a         5831 c         5605 g         3508 t
ORIGIN      
        1 acaaagacga aggccggcgg catcagcgcc agcagcggga gcaccagcgc gagccgggtt
       61 gagcggcgcg ccagcgcgaa tgccagcagg ccgaagccca gccagtagga ggtcgcgatc
      121 agcaggaaca tgctgcccgc accgggcgcc accggatcga tcacgcccca cagcacggtc
      181 atgaccgggg attgccagtc gccgagcacg cccttggcga tgtcctcgta gacgaatttc
      241 gcgtcgtagg tgaggatgcc cggataaaag atcgttagcg tcagaccgta gccggcggcg
      301 atcagcgcgg cgaccgcgag aaccaacctt cgccacatcg gcggcatgcg gtcagccgag
      361 ggcggcatag atgtccccgg aattggccgc gtgcggcagc ccggtcagcc agcgggcgat
      421 ctcttcgccg ccgacccaat cggcgttgac aaacacctgg ctctcgccga gcgcggcatt
      481 gaaccgcgtg taaccgagcg cgatgcagcg ttcgatgcag gcgagtgcga catggcgctg
      541 gatgatggtg aactcgaacg acagcgcctt gaccgcgacg gagagcccct gcagcgcctc
      601 ttcctcaaag ccttcgacgt cgatcttgat gaaggctggt gcgccgtact tgccgatcag
      661 cgcatccagc gtcgtcaccg gcaccggcat cgacttgatc cagcgctgcg tttcccagcc
      721 ctgcgcatcg cgtgcggcgg ttacgaactc acgcgacgcc gtcgaaaccg taggattatc
      781 ggcattgatc attaggctgg tcgcgccaag gctgcggccg accgccaccg gctcgatcgc
      841 gacatcggcg ctgcgcccgt agaacagctt gagcaccttg agcatggcgg gctgcggctc
      901 gaccgcgacg atccgtgcgc cgagccggcg gaacgaggcc acgcggtcgc cgacatgggc
      961 gccgatgtcg aacacaagat cgccgtgtcg caccaagccg ccatagagcc ggtccatcgc
     1021 cgccgcgcgc gacctgtcgc cgtagtagat gcgaagcgac cgaaaaatac cgcgcgcggt
     1081 cctgaaaccc tcgaacaccg cctcagaccg aacttggccg cggcaacggc gccgtctgct
     1141 caatcgcggg cagcgccaaa gccaatagca ccacgggcct cgtggtttcc tccggcagcc
     1201 ggccaatgcc gacccgggcg gcaaaatggc cgagccgagg ccacgcggtc atcatggcaa
     1261 tcgggatcgc caacgccggg ccgccggcga gaaacagcgc atagggaacg gccgccggct
     1321 gtgtcaccgc cagcaggccg agtgctccgc agccgagcag cgtgtgcggc cacaaattgc
     1381 gcagcgccag cgtgaatggc accgcgtgat cgtcacgggt ctgcccgatc cagccaattt
     1441 cgcggccgaa caaaagtccg gtgaggaaga tagtatggcc gaaccacagg atcggacaga
     1501 gcaggatcga gtacacggtt tcgacggcgt agctgacaat gaacaatccg gcgccgccaa
     1561 acccgcggcg aagctccggc cgcatcaaga tgtcgacggc gctcgcgatc ttcggcgaga
     1621 accacatcac cagcacgcac acgaacagcg ccatgccggc gtcggcgcga atgaagtttt
     1681 ggggcgcatc ggagaatgcg acggccagcg tgccgatcac gagcaagccg atccaggccg
     1741 gagagccgat gaacatcagg atcgccagca ccaactgata gcggctgacc ggcttgaggc
     1801 cggcaagcga gagaaaacgc caatactgca tgttgccctg gcaccagcgc agatcgcggc
     1861 ggacgaattc gagcaaggtt ggcggatttt cctcccagcc cagatcctct tgcggcagca
     1921 cgcgcacatc atagccggcg cggcgcatca gcacggcttc gatctgatcg tggctgagga
     1981 tatggcgatc ttccgttccg tcgcttgcta ccggaagcga gcaatgcgcg atgaatggct
     2041 caagccgcag cgccgcgttg tggccccaat agggtccgca atcaccctgc caccaggcgc
     2101 tgccgatggt gtaggagcgc atgccgagcc gcatgccgaa ctgaaacacg cgcgcgaagg
     2161 cgctggtcga cggcagcccg acgatcagcc cctgaaggat gccgagcctg ggatccgcct
     2221 gcataatgcg gacgagacgg agaacggcgt cggccgtcat caggctgtcg gcgtcgagcg
     2281 tcaccgcgaa atcgtgttgg cggccccagc gctcgcagaa ttcacggacg ttgcccgcct
     2341 tgtagccggt gttgaccgcg cgacgccggt aggtgatcgc gacccgatcg cgccagcgcg
     2401 cgatcaagct ggcaaagcac gcctcctcgt gatcggcgat tgccgcgtca gaggtgtcgc
     2461 tcagcacata gagatggaac cgctcgccgc agccggcggc gtcgaggccg gccagcatcg
     2521 gctcgagatt gcggatcatc cgctccggca actcgttgcg ggtgcacaac aggatcgcag
     2581 tcgatgccgt caccggctcg tcgccgcgga tgcgcgctgc cgccggcatc accgccgcga
     2641 tgggatcggc ggaaaaacgc atgatcagaa acccgatcac ggcgttccag aatcccgcca
     2701 ccatccacgg cagcgtgacc gcgaacagcg ccaacagtgt catgtcgatc acgccgaagc
     2761 cgcccggcgc cagagccagc gcggccagcg ccagggatcc gaccatggtt gtcgcaaaca
     2821 ggccagcgaa cagcacgcgg cggccgaaaa tatcgttttc ggtggcgtcg gcctgcatga
     2881 cttgcggggg ggatcgttct aacacgcagg tatgacttct aatccttcga cagtgcaaac
     2941 tcgatgatat aacaccgcga acgatctcaa agtccaatcc ggggataaag taaagtggcg
     3001 atcagccggc ccgaacaggt tgcccaggcg ctgtggtata gcgggcccgg acaagccgac
     3061 atccgccagg aaacacttgc tccgcccgga gcgggcgaag tacgcgtgcg ggcgctgttc
     3121 ggcgcgatca gccgcggcac cgaggcgctg gtgcttgccg gacgcgtgcc ggagagcgag
     3181 ttcgaacgca tgcgcgcgcc gttcatggcc ggccattttc cgtttccggt gaaatacggt
     3241 tacgccacgg tcggccagat cgagggcgga acggaagccc tgctcggacg cacggtcttc
     3301 acgctgcacc cgcaccagga tgtcttcaac gtcccggcaa gtgcggccgt ggtgctgccg
     3361 aaaaatctgc cgccgcaacg tgcggtgctg gccgccaaca tggagaccgc gctgaacgcg
     3421 gtctgggacg cggcgccggg cccggccgac cgcatcgcca tcgtcggcgc cggtgtggtc
     3481 ggttcactgg tcgcgtatct ctgcgggcgg attcccggca ccgaggtcac gctcgtcgac
     3541 atcaacccgg cacgcgcgga attggcgaaa acccttggtg tcagctttgc gaagcccgaa
     3601 accgcaaagg gcgactgcga tcttgtcgtt cactcaagcg gccatccggc cggacttggc
     3661 acggcgcttg cgctcgccgg tgaggaagca accgtgcttg aattgagctg gtacggcgac
     3721 gcgtcggtgg cggcaccgct cggcggcggc ttccatagcc gacggctgcg gctggtgtcg
     3781 agccaagttg gacggatcgc gccgtcgcac cggccgcgct ggacgcatgg ccggcggctc
     3841 gccgcggcgc tggatctgct cgccgatgca cggctcgatg cactgctggc accggcggtt
     3901 gtattcgaag atttgccgcg gcggctagtg gatattcttg acgccagccg tggcgtactc
     3961 tgccagctca ttgcttatcc ctgaaggagg tcccttgttc gctgttgaag tccgcgatca
     4021 catcatgatc gcccattcgt tccaaggcgc cgtgttcggg ccggcgcagg cgctgcacgg
     4081 cgcgaccttc gtcatcgatg cggccttcct tgccgatacg ctcgatccca acggcatcgt
     4141 catcgacatc ggccgcgcgc acgacgcgct caaggccatc gtggcgacgc tgaattatcg
     4201 caatctcgac gacgtgcctg aattcaaggg ccacaacacc acgaccgaat ttctgaccag
     4261 gcatattttc gatcggctgg ccgaagaggc ccgcgccggc gcgcttggcc gcgatggccg
     4321 cgagctcaag gcgatacgcg tcacgctctc cgagtcgcat gtcgcgcgcg cctggtacga
     4381 ggcggatttg tggtgaagcg tttcgcgttc gccgtgcccg gggatcttgc aaccccgacc
     4441 ggcggatatg cctatgaccg gcggatgatg accgagctct gcgatctcgg ctggcagatc
     4501 gatctcctcg acctcggcga aggctttccc cggcccgacg aggcgacgcg cgcgacagcg
     4561 cagtcgcggc tactcgcgat gcccgctggc cgatgtatcg tcgtcgatgg attggcgctt
     4621 ggtgtcttgc cggaaaccgc gtcacaactt gccggccgca atccgttact ggcgctggtg
     4681 catcatccgc tggcgctgga atggggattg tcggtcaagc aggccgacac gttgcgcgcg
     4741 agtgagcagg cggcgctcgc agccgtgcag ggcgtcgtcg tcaccagcgc ggccacggca
     4801 aaactggtcg cctccgatta cggcgtgccg gccgagcgca ttgccgtggc aaggccgggc
     4861 agcgatccgg cgctgatgtc gcaaggcagc caggacggcg tcgtgcggct gctgtcggtc
     4921 ggcgcaatcg tcccgcgcaa gggttttgac gtgctgatcg ccgcgctggc gacgctcacc
     4981 gatctgtcat ggcggctgac gattgccggc gaccgcacgc gcgaccgcaa cgccgcggca
     5041 cggcttgatg ccggtatcgc gcgtcacgcg ctcggcgatc gaattgcagc gctcggcgcc
     5101 gtatcgccgc agcgcctcgc ggcgctctac gccgaagccg acgtgttcgt cctcgcatcg
     5161 catttcgaag gttacggcat ggcctatgcc gaggccgttg cgcacggcct gcccgtcatc
     5221 ggcaccagcg ccggtgctat tccggacacg gtgccgccgg acgccggcct gctcgtcgac
     5281 cccggcgata gttttccatt ggcacgggcg ctgcgccgca tcatcggcga tgccgatctg
     5341 cggcggcgcc tcgccagtgc cgcgcgcgca gcggcaccgc aactcccgac ctggcggcat
     5401 tccgcggaaa tctttgcgcg cgcattggag acgctggcat gagcggcttt tccgccgact
     5461 ggctgacgct gcgcgagcct tacgacttgc gcgcgcgcaa tccgagcgtt ctcgacgctg
     5521 tagccgcctc gctcgaaccg ctttcctcgg tgcgggttgt cgatcttgcc tgcggcaccg
     5581 gttcgacctt gcgcgcgctg agcccacacc tgcccgcgcg gcagaactgg aagctgatcg
     5641 acaatgatct cgggctgctg gcgcgcgccg aggccacgcc gcttgccaag catgccaccc
     5701 ttgccgctat cccactcgat ctcaaccgcg atctcgaagc cgcccttgac gggccggtcg
     5761 atctggtcgc gacctcggcc ttgctcgatc tggtttccga aacctggctg gaccggctcg
     5821 cggtcgagat cgccgcgcgc tcgatcccgc tttacgcggc gctgagttat gacgggcgga
     5881 tcggattcac gccacccgat ccgcgcgatg cggccatcgt tgccgcggtg aacgcgcatc
     5941 agcgcaccga caaggggttt ggaccggcgc tcgggccggc agcggccgcc ttcgccatcg
     6001 cgcgcttcga ggcgctcggc tattcggtgg tgcacggcgc atccgcctgg acgatgggac
     6061 cggacgatct cgacatccaa atcgaaatac tatccggatg ggcatcctcc gcgcacgaaa
     6121 tgggcgcgct gtcgctcgcc gacacaacgg cctggcttgc gcgacgcggc gacgccgttg
     6181 ccgccggatg ctccacgctc catgtcggtc acgtcgattt cttcgcgacg cagagcgcca
     6241 cgcgctgagc cgacagatcg cagtcgaaca gcacatcgtc ctcgatttta tgcacacgca
     6301 cgggcatgcg tggcgcctga tcggcgcggt cgagcgacgg caggatcagg ccgggtcctc
     6361 ctgttcccag gatgattggc gcgacgatca cgtgcaggcg gtcgagacac ccggcgacaa
     6421 gaaaacgcga taaggtgtcg gcaccgcctt cgatcagcac acggcgcatg ccgcagcggc
     6481 cgagagccgt gaggatcgct gaaggggcaa attgtccatc ggccaccggc agcgtgacaa
     6541 cctcgacgcc cgacggtgcg gtgcagcgcg taccttgctc ggtgatcaac aaacggcgga
     6601 caccgttgtc ggcgaacatg cgggcgtcgg caccaagccg gcccttcgga tcgatcacga
     6661 cgcgcgccgg ctgaggcccc gagacccgcc gcaccgtcaa ttgcggatca tcggcgacgg
     6721 ccgtgccgac gcccacgacg accgcgtcca ccagggcccg cagccggtgc agatgtacca
     6781 gtcccgccgg cccgttgatg tattttgaat ggccggattc cgtcgcgacc cggccgtcaa
     6841 gtgactggcc gatctggccg acaatcacaa aatcgtccac cgtgcccttc cgcagcgggc
     6901 cgaacacatc cgcccaggcg tgcggaagag gctggcgctc tcgacggaat atatcgctga
     6961 agtcttccca cggatcggaa gacacagatt tactcgaggg gaaattgctg atcggcacga
     7021 agaaatcaag aaccctgtgt ggttgcacta acaatacgac tatgtgccat catgcaacaa
     7081 aaatcattcg atttcgcggc tggaatgaag ttggacgcgc ggtgttctac gaacgcggca
     7141 acaagaatgg caagaatcga aagtcccgga acaagaatgg aagtctcgta atgaatgaag
     7201 cgcagacggg cgcaagttcc gctctatttg gcagccgcag ccacatcagt gtgagccgcc
     7261 gcctcggcga attccacgga cggcggcctg ttctcttcac cgcaacaggc gaagccgtgc
     7321 tcgccttgcc ggtcgaaggt ctcgacaggc ggcggcttct tgaattcatg gcactctgcg
     7381 caccggtcat gccgcaactc atcatcacgg agcgacgcgc gctcgcgctc ggccttgatg
     7441 cgtcaacgcc gacggcgttg acgctctcgg caggtgacaa cgccaacacg atcctcgccc
     7501 tcgtgaccga cgcgacaaac caccgcatgc cggccgccac gccggcgagc ccggccgccg
     7561 ccgctgccat ccagcttgcc aagctatcgc aaagcctgcc ggccgtgctg gcagccgatg
     7621 tggttgcccg cgccgtcgcg tgtgaggaga gcatcgtcag ggttgaggct gattcggtgg
     7681 ctagctttgc cgatgatgcc atccgctcgc tgatcgtggc aagcgaagcc tcggtgccgc
     7741 tcaactcggg cacgcccgcc cgctttgtcg tgttccgcga cgccatgggt ggcagtcagg
     7801 tcgccatcat cgtcgggaag cccgatttca ccaacgccgt tccggtgcgg ctgcattccg
     7861 cctgcctgac cggcgacgtg ttcggctctc ggcgctgcga cggcggggac cagcttcggc
     7921 tcgctgtggc gctgctccaa gacctcggcg gcggcgtcat tctctatctg gcgcaggaag
     7981 gccgcggcct tggtctcacc aacaagatgc ggacctatca gttgcaggac gacggcctcg
     8041 acaccttcga cgccaacacc acactcggct ttgacgacga tgagcgcgat tacggaatcg
     8101 cagcacatat gttgcggatg ctgaactgca cgcggatcgt gctgctcacc aacaatccag
     8161 ccaagctcga cggtcttacc agggccggca ttgaaatcgc cggccggatg ccgctcgacg
     8221 cgccggtcaa tgccgacaac cgccgctaca tgacggcgaa ggcaacgcgt gccggacctc
     8281 agttcgatca tctgacggcc tcgctggccg accaggccta ggtgacaaga gcgggacccg
     8341 aaggcggcac gacgtcgcgg tctaaaatcc gctccgactc cgacatgttg cgcgccttgg
     8401 tggtcggaac cggtctctgc tggtcggtcg cgttcgtcgc actcgcgcta tgctatgagc
     8461 tggagcttta tgcggatggc gcgatgtttt cctatgccgt cgcggtccag gacgtctggg
     8521 cctttcactg gcacaatatc tccggccgcc tgtcggtctt tgtccttagt ctgttgccgg
     8581 ccgagatcta tgtcgggctg agcggcaacc ccggcgccgg gatcgtcgtc tatgggctat
     8641 tgttctatct cgcacctctg gcgggactga tcggaacttt cgcggccgac cgctcccatg
     8701 gccgcatcat cttcgtctat gcttgttgct cgaccgcact gctctgcccg ctgatctttg
     8761 gctttccgac cgagatgtgt ctggcgcacg ccgtgttctg gccggcgctc gccgtcagtc
     8821 attatgcgaa accaacggtg gccgggacag cgctcgtttt cgtcatgctg ctcactctgg
     8881 ctttcaccca tgaaggcgcg ctggttctgg cgttcgcgat cgttgcaaca ttggcgccgc
     8941 gtggcctgcg cgacgccttg tttctgcgcg cggctgcagt cctggctgtg gtcctggtga
     9001 tgtcggccgc cgtgaagatc atgattccgc ccgacgaata ttatgccggc gtgctggtaa
     9061 gggccgcgct gaacttcttc gatcccgcca tcttccaggt cagcatcgtg ttgttgttgt
     9121 tcgcgacgct cgccgggtat gctggtatca acctgatgct ttcgcggctg gcgccgaacc
     9181 gggcgtatct ccatgccgcc gccatcatga tcgccgttct tgccgtctat tggctgtggc
     9241 tcgaccacac gatacatgcg agcagccggt attatctgcg aacggcgctc gtggtcgtca
     9301 cgcccgtgtt cggcgcgctg gcggcgctgg gcgccatgtc cggagatcgc cggctcgctt
     9361 ttccgttttc gagcctgcaa caggcaatga ccgcgctgca aagacgggcg gcccggcccc
     9421 ttgcagtggc gttcatgctg tcaacgctgg tgcacgtggt cgagaccggc aaattcgtcg
     9481 cggcgtggac ggattaccgc gccgcggtca cgtcgctcgc gacgggcgat caatccgatc
     9541 cggcgctcgg cgatccgcgt tttgtttcgt cggaacgaat cgcctcccgt ttgaaccggc
     9601 tggcctggtt ctcgaccaca ccctacctgt ctgtgatcgt tgcgaacttc atgcctggtc
     9661 ggctcgtgat cgatccgact ggaaattatt tctggttatc ctgcgccacc gcaacggcga
     9721 atgacaaggc cgcccgcgcg gttccggagg agggtcgcga tctcctgcgg atttattcgt
     9781 gcctgcatcg ctaggccacg acgagcttga cgcgtttgct gcggccggga tatccccaaa
     9841 tggcccgttc gccagccgca caattgcaaa gccgtgtcag caattgcata gttgagcatt
     9901 ggaacgtcct gatggcattt gaggaaatcg aaaatggcgg aagccgatct ggatgtcgta
     9961 attcggcagc ttgcgaagca acaaaacaaa ggtctcatgg ccgccgccaa aagccggcgc
    10021 aatcgataca tcggccttgc cgccaaggcg aagaataagg aggccaagga acgatacaag
    10081 cagatcgcga agaacatcat gctgcaagga actgccgccg ccaagagatt gcaaatttct
    10141 gccgataacg ccgccgacag ctacgccagg tccatgcgac aagcggcaga ggctcaggcc
    10201 actcaaaagg cggtcaagaa gacaaaaacc tgatcgaggc cggcacgacc ggaaagcatg
    10261 ttggtcaaac tgctggcatg ttgcggccag cgacgctgcg gggcttgcgg cccgcttgcc
    10321 ccaactcccg attgaaaagc gcgcgctagc ggcgaccgct ggcggcaaac ggagacgcgc
    10381 cgggcagcga gagcgaattg atgtcgctat cgatgcgccc cgactgttgg gtgtagccga
    10441 acccgagtga caggctgaca ttcggcgtcg gctggaattc gacgcccgca tgcgcgctat
    10501 agcctggcag tgtaccggac gtgctgtcga agggggcaaa agggctaccg atgccggcat
    10561 tatatttcaa ggtgtcgaaa ccggcataga tcgtgagggg tgcgttttgg aagttgtagc
    10621 cgaagtaata aagcgagccg atattgccga acgctccatc ccggttgatg ccgttcatgc
    10681 tcaaacccat gccgccgcct tcgccgccga caaaccagcc gttcggaaaa ttgtagcgca
    10741 tataggaaaa gccgccgcct ctggcgtcac tgccgtcaaa gctcggaaag ttgccgtaag
    10801 cgttcgagtt ctggccgaca gttggattgc caccgaagcc gacgggccag gctggaatcc
    10861 aatagttcac gggggcggcc tgggcatggg ccgttcgccc gcccagacag agcgccgcca
    10921 ataccaatgc gagacctaat ctgcccgata aaatgcacat ccgcgccacc gccaagtgtc
    10981 ctgaaactat acgatctgga tatcaaaaac gccagtgcgc cgccgctggc cattcgcgca
    11041 tagcaccgga tcaggcaccg ggaacaggga tgccgggctc gccgcttgct cccgcttcca
    11101 ggcccgatct gtttggccat ccgttcttgc cccaacggca ccggacttct gcgggatttt
    11161 catttgctac ttcgatcgct ggctgatcga catgacatac gcggtcgctt tccaaatttc
    11221 ttcatcggtc agcacttcgc gccacgccgg catgcggagg ccgctcttct cccggccgtc
    11281 ggcaatcgat tggaaaacgc ctttggggtc gagatcgcgt cgggccagat tgatacctcc
    11341 cctgccgtct accccgtgac aatgcgcaca ctgcttttcc agaaagacat cgtatccgga
    11401 cgaaatcatc ttcgggtctt tgaggtccgg gacgttctct tccgaatagg ccacatcgcc
    11461 gataaaaatc catgcaacca ccaccgtcac caatccgcgc attatggcaa ctactccaac
    11521 actgagcgaa ataaaagcca agccgggcgg ctggataccg gccgcccgtt cgttgcttgc
    11581 ttcgcttacc tggcaagttt gaaggcgatc agcgccgaag ctgctggtac cttttcaagt
    11641 tgcgggaaca acgccggaag caagaccgcg gcgtacgaac cccagccact tggcaccaag
    11701 atgtactgct cgccgtcgac ggcgtagctg acgatgccgc tgcgcattcc ggagcctgta
    11761 ttgaagctcc acagcacctt tccggtgtcg gcatcgaagg cccgcaggat tcccatcggg
    11821 tcgccgttga acaccaatcc gccgcctgtg gtcaggacgc tggcgaagcc gggaatgtcc
    11881 atctcatacg tccatttgcg ctcgcccgtg aatggatcgc gcgcatcgag ccggcccggt
    11941 ttgcgcccat cgggtgccat cgccaaccgc ccgaaatcat tggagccggt atggccggtg
    12001 ccgtaatcct tggggtcctc tttctgctca actggcttga ggtagccgca gaaatccagc
    12061 acattgttgt accaaagtcc ggtcttcggg ttgtaggcac ccgaattcca gctacgcccg
    12121 ccaaaggtgg acgggcagat cagcgtctcc tgattcatcg ccaattcgac acggccgatc
    12181 aactcgccgt tttccctgtc gatgtccttc acaaagttct tgatgtcgct gaccggccag
    12241 atgttctcga tgttgccgct attcttttcg agcacgaaga cgtagccgct cttgttcatg
    12301 tggacgatca cgtccttgcc gttcttcttg aacagcatca cctcgtaagg cgaatcgtag
    12361 tcccacacgt cctgcttgat ctcctgacgg taccacttca ggttgccggt cttggcatcg
    12421 agcgcgacga ccgaatcggt gtagaggttg tcaccctgcc ggtccgagtt gacgaaatct
    12481 ttccccgggt tgccggtgcc gtagaagatc gtgttcgtct ccgcgtcgta ggtgcccggc
    12541 atccaggcgc cgccgccgcc atatttgccg ctctcacccg gccaactctt ctggtcgttc
    12601 ttgatggtgt tgaattccca caccttcttg ccagtttcgg cttcgacgcc gaagattttc
    12661 ccttgcgtcg ccaattcgcc ggcagtcgag ccgaaagtca ggatgtcgcc ggcaacgacg
    12721 ggcggcgacg tgaaattgca gccatggcag ttcgcaaaat cggttagctg gacctgccat
    12781 ttttccttgc cggtcttctg atcgagggcg atgccgcgcc cgtccaccgt gccgatgaag
    12841 accatgccgt gcccgaccgc gacgccacga ctatagggtg agaacaacac cttcttcgtg
    12901 aggggatcga gcttcggttg aaagctccag agctcctgcc cgctctttcc gtccagcgcc
    12961 gccacccgat tcccggacgt gattgaataa atcacgccgt ctatcgccaa tggcgtttct
    13021 tgaatgccca tggtgatatc accgccgtgg gcgatccagg ccaccgaaag cttggtgacg
    13081 ttgtccttgt tgatctggtc gagcgggctg tagcgccacg cgttggtggt ccgatgatac
    13141 tgcggccaat tgttggggtc ttcggtgccg gggtcggcag ccagggcaac cccagccgtg
    13201 cacagcaagc aagccagacc gaagcttgcc gccaaggtcg ttttagccat tgtcattcct
    13261 ccctcagatg gtttgatttt catcttaagc cattccgtta cctccgaagt ttaaggcgca
    13321 gccataactg accctgcgaa tacaacgcag tggcacgctg gtcattgcag ggttcggtag
    13381 tggcaggggc tatccgaaag cgaggccggg tcgctgtagg tccgggcgat cttgatttcg
    13441 gtgtccgtca cacccggcct gcggtgcttc tgcgcagatg ccggaaacgt aagcgcgacc
    13501 cactaccccg cgcacagggc gcggttctcg gcgtctctcc ctcggcagct ggaagtcgct
    13561 tcagagtgcc attgtgccgt gctcaagtca acgatgcgct ccgatcatcg agcctgccat
    13621 gaagctgccg cggctggtga tgaacgaagc taccgtgcgt gcgtcatgcg ccaacctgcg
    13681 acgatgactt tggggcgtga ggtcctgatc ccaagcgcgc actgcttatc gatgctggcc
    13741 aaaccgcaac ttccgctccg agtccaatcg cgtcgatctg gcgatggcag agttacttcc
    13801 gcttttcccc tgatagccga cgttcgtcag agcggtcggc aactccgaaa agtgccgata
    13861 accgaagtcg cgatctcatt cgatcgccgt gtccgcgcgt gagagcagcg tcggcgggat
    13921 ttcgacaccc agcgttttgg cagtcttgag gttgatgatc agctcgaacc cggtcggctg
    13981 ctcaaccatc acatcgagca ctttggcgcc tcgcaacagc tttgcgacct gacgcgcgca
    14041 ttggcgaaaa actacgctga gacggggatc caaccaccga ctccaaaaat agttctgtcg
    14101 cataatcatg atgactctga cgcaagagaa gaaataaggt gtgaataacg gaggacaacc
    14161 tatgggtgag aatcattgca cgcgaatgga acagcattgc ctggagcggg cgaggtttga
    14221 gccccggaat aggggaaaat ggattgctca ggccgagcgg tggcacgaac tggcacgcgc
    14281 gcaaaactcc tggcgactcc agaagaaccc tcttcaacag tcgatgcaga caggcccaat
    14341 ggcaacgcag cccaatgcga ccaatactcc ctggcaacag ggctaaccgg ccaactttgc
    14401 cccctgaaga atgagtaacg gcccagttca agtgacttgg caaatccaaa caacggtcaa
    14461 gtcgaatgat acgcgataga acgcgattgc cgccgcgtga gaactctcat gcgagcggct
    14521 tgacgggttc catcgcgctc aaggaagaat ttgacggtgt cggcccggtt gtctcttatc
    14581 ggctaacggg tagccggggg agcgaccccc ggcccctcgg cctaacaatg ggctggctac
    14641 tcaatcgcac attggggagc gaccccgtgc atcaaggatc gtccatgccc gagggtcaga
    14701 ccaaccctca attacacaat accatccctc aacaagtccg ccaaattgaa acggcttgat
    14761 ccttaccgat tctccggaac gcgatgcctc tgctttcgca cgcttaaccg cgtctgagcg
    14821 catgggcgac ggccgccgtc acgggttccg gcgcggcagt gtgagagacc ccggcgcttg
    14881 tcagcgcggt atccgaatag atttcgaagg cgatggcgat caacgcgagc gccaagatga
    14941 cggccatggt tctcatggca gcattttccg cacgcgattg aggcagaatc agtcagataa
    15001 cccggcgaaa aacggaagtt acatttcgga gggtccacgc caccgcctct ctgccggcgc
    15061 atgcgacccg tcgctctccc gggcatcaat acgtctgggt cagataatcc acaatcttgc
    15121 cgacgtctgc ctcatcgatt ggcgcaccgt aaaccttgat catcttggtc acctcggccc
    15181 gccagaagat ttccttgaat ttcggacccc ggggctgggt ctggacatag tcgaccgaat
    15241 ggcacgccgt gcagttgttc tgcacgacct ccagattggg acccggcttg aatgtcgccg
    15301 tctggttcgg aagcttgtac gcgaccggct tggcgtccgc ttcggacgga gctgaaccgc
    15361 agatgccgat gatcagtccc gcgaggccag tcagcgccag tgcgagcgtc gatagcttca
    15421 tggccgtctc ctcaagccgc cgtgacgcgg acggtttcga ccacgttgcg caggtagccc
    15481 gccccgttcc agagcggctc catcggctgc gtctcgccgc cgttgctggt cgcgcgcacc
    15541 ttcagttcgt gcgcgccggc cgccagcctg accggcatcc gccattcgcg gaaggagtat
    15601 ttccccagat tcctgcccag gctggcctgc gcccaggttt tcccgccgtc ggtggagacg
    15661 gcgacgtcct tgatgccctt gccgccgtcg aatgcaatgc ccttgagcgt ggtctgcgcg
    15721 ccggctctca gcttcgcgcc gtccgcgacg ctggtgatga acgaccttac cttgaagcgg
    15781 ttgatcggaa cggtggcttt cggggctgtt ccgggctcca cgcaattgca gtcgttgtcg
    15841 gggatgcgat acgcggtctt catccaaaat ccgtcgaaga cattgtcgat gaccgtgatt
    15901 tcgttgaggt gcttcaccca ataggtaccg aaatagcccg gaacgacgag acgcagcgga
    15961 aacccgttga ggaccggaag atcagcgccg ttcatcgaat aggcgagcat gacctcgccg
    16021 tcgcgcgcat ggtcgatgtc gagcgccttg acgaaatccg gagtcttgtt ggtcacgggc
    16081 ccgtccatgc cgttgaaggt gacctggcgc gcacccggct ggacgccggc cctgtcgaga
    16141 acggccttga gcgccacccc gcgccagcgc gcattgccca tggcgccgtt ccccaactgc
    16201 ccacctgcga cgcgaggatt gaagaatccg cggctgttgc ccgagcactg gttgaccgcc
    16261 acgatgtcaa cggccggcat cttcttgatg tcggccagcg acagttttat gggtttgtcg
    16321 accttgcctt ggatcccgac cgagaacgcg tcggggtcga tgttcagcgg gacgtccgcc
    16381 agatggtagc ggacgaaaaa ggcgtcgttc ggcgtgatga cattcgcatt gaaggccgag
    16441 aacggcgttt ccagctgtgg cggtctgctc gtcaggccga tcatcggacg cttttgcgga
    16501 tatttgacga gcggccgctc gccgttgtcg aacggcagcg ccgccgtggc tgcaaatgcc
    16561 cgtaaagagg gggatatcat tccggcaccg agcgccgcaa tggcagcacc ggaatgcttt
    16621 atgaagctcc gccggtcgat catcgcattt cctccttttc gattggtcgc cgcccaccgc
    16681 gatccggtgc cgcctcgaag cttcagccgt cggctccccg ttcgtcccta ccttgaggag
    16741 tcccgagaac cggcagccga cacctgtcct ggtggaagcc gcagcggata tctttctggt
    16801 gacattcttg cgcgtctaat gtgccgccgc ctgctcgacc ttggactcgg caagaatttt
    16861 catgaatcgg gcgaaaagca tttcccgttc cttggtggag agcgtcttcc gaccggacga
    16921 agcggcgtag ttctgcatga atttctcgaa tgccagcctc agctcgggag aggatgggcc
    16981 gagcgtgccg tctggattgg cggacacggc ttgatttcgg tgggcggcaa gccattcggc
    17041 cgccggctta aatcgcaccc agccaggcat ttcggccgaa agattgactt cgcgccactt
    17101 gggatgcctc gacgggttgt tgaattgatc gatcttgctg aaaaacgcgt cgatgaattt
    17161 ggcgaccttc ttgtaccgcg gggaattctc cggccaggta taggcgacaa gcaatgcccg
    17221 gtttgcaatg gtcgggaccg tggtcccctc ggcgatcaga ttcggatagt tttcatgggt
    17281 catctccgcg ggaagataac ttgcgaacaa atcacgcaag tcatgatttt gcaggctttc
    17341 ctgatcgagc gggaggagat gcacgccgtc gtccttcttc accttcgcca ggagggcctg
    17401 tggcgctccc gtgaggatga tcattgccga gatttctccc gacatcagcc gcttgagtgc
    17461 ttcatcgttg tcgtagtaag agcgctgcac ggggatgttg agcacactga aaacggtgtc
    17521 ggcggtcact tcggtctggc tgtccttcag gttgaagttg accttcctgc ccttcagatc
    17581 gtcataagac ttgatttcgt ttcgcgcgag aacatgaaac tcggaattgt agagcttcgt
    17641 gatatattga acgcgctgct cgacattggc atacagcacc ggatccttct tcttcaggag
    17701 tcgaagattg tcctcgtcga cgacagccat gtccatgttc ttgagaaaca gcacatcatt
    17761 cacgttttga agtccgccga ccccaagaac cggcagaacg cgcaccccgt ccggccccaa
    17821 atcgttcacg acgttccgga tgtcctcagt gaacctggcg cacgtacatg agatgccgga
    17881 caccacgacc gttacggcga tatcgttggc ctgacgcttt ttttcatcgt aactgaccgc
    17941 cggcttttgg ctttgagcca ccggcttttg gctttgagcc aaaacaggac gcaaggggac
    18001 agttaaggat ataagaaaca agaagaacga caggcacacg aagagatttc tagcagtcgc
    18061 ggtgaagacg cccataactt tgccctttgc tgtgatctgc ggcccattaa gtgcgtgccg
    18121 cgaagtgagt gccgcgtccg aaattcggtt ggacttggtc tgttcggttt gcggcgaaca
    18181 tccgtcggca gtaaacgtgg ctttgaaacg tgtcatacgg tgcgccgatt attgccgctt
    18241 gcttgcgagc agttcgagcc gctgaggcgc ctccgccgat ccgaactttt tggctttctc
    18301 gtaccagact cgtgccatgg ctacgtccgg cacgaagcca tggacgccca gtttctccag
    18361 aatcaccgga tcgtaggttc cggcgagcgt catggcggcg cg
//
LOCUS       DATNOI010000414         2565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_625269,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000414 DATNOI010000000
VERSION     DATNOI010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625269"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VN968_16650"
     CDS             complement(<1..506)
                     /locus_tag="VN968_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL30898.1"
                     /translation="MADGTIGLWTVPLAVFGGAIRVSTPFLFVSLGECITERSGRINL
                     GLEGTLVMGAMSAYGISYLSGSPWLGVLAAGVTGSLLGALHAGICSLPRVNDIAVGIA
                     LMLFGTGLAFYLGKPLIEPTAPRLPAIDFGWWSDVAQVRAALRINVLFLIGVALAPVL
                     YWAFRTTR"
     gene            complement(508..1617)
                     /locus_tag="VN968_16655"
     CDS             complement(508..1617)
                     /locus_tag="VN968_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL30899.1"
                     /translation="MTSGVADPLEAAAISPAADPGFLQRYGGIIEYIVIPGAALVGAL
                     GVFGIFVALFGKSPLDLYFYMYQGAFGTWFSWQNTLTRAAPLILTALCTALPAQLGMV
                     IIGGEGALLIGALAATSTALVMQSAPPLIVQLAMVCAGMIGGGLWILLAGGLRQYRGV
                     NETISSLLLVYIALAILNHLVEGLMRDPASLNKPSTREIGAVNMIGTIPGTDVHWGLV
                     FGIVAAIASYVLIYHTVFGFAARVAGGNIRAARIVGLNVGRLILMICFLAGAAAGLAG
                     MVEVAAVQGRTNANLAAGYGFTGILVAFLARQNPLAVIPVAILLGGISASGGLLQRRL
                     GLPDASVLVLQGIIFVAVLASDALYGRIGFLTGKT"
     gene            complement(1614..2565)
                     /locus_tag="VN968_16660"
                     /pseudo
     CDS             complement(1614..2565)
                     /locus_tag="VN968_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612801.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
BASE COUNT          495 a          798 c          811 g          461 t
ORIGIN      
        1 cagcgcgtgg ttcggaatgc ccagtataag accggtgcca gagcgacgcc gatcaggaac
       61 agcacattga tccgcagcgc cgcgcggacc tgcgcgacat cgctccacca tccgaaatcg
      121 atcgcgggca accgcggtgc cgtcggttcg atcagcggct ttccgagata aaaggcaagt
      181 ccggtcccga acagcatcag cgcgatgccg acggcgatgt cattcacgcg cggcagcgag
      241 caaatgccgg catgcagcgc gccaagcagc gagccggtga cgccggccgc gagcacgcca
      301 agccagggcg agccgctgag gtaggagatg ccgtaggcgc tcatcgcgcc catcacaagc
      361 gtaccttcga gcccgaggtt gatgcgtccg ctgcgctcgg tgatgcattc gccgaggctg
      421 acgaacagaa acggcgtgga gacgcggatg gcgccgccga acaccgcgag cgggaccgtc
      481 cagagcccga tcgttccgtc cgccatgtca ggttttgcct gtcaggaagc caattcgtcc
      541 gtatagcgca tcgctggcga gaacggcgac gaagatgatt ccctgcagca ccagcaccga
      601 cgcgtcgggc aggccgagcc ggcgctgcaa aagcccgccg ctggcgctga ttccgccgag
      661 caggatcgca accggaataa cggcaagcgg attttgccgc gccaggaagg cgacgaggat
      721 gccggtaaaa ccatagcctg ccgcgagatt ggcgttggtg cggccttgca cggcagcgac
      781 ttcgaccatt cccgccagac cggccgctgc gcctgccaga aaacagatca tcaaaatcag
      841 tcggccgaca ttgaggccca cgatcctggc ggcgcgaata ttgccgccgg cgacgcgggc
      901 ggcgaagccg aacacggtgt gatagatcag gacgtaggaa gcgatcgcgg cgacgatgcc
      961 gaacaccagt ccccaatgca cgtcggttcc ggggatggtt ccgatcatgt tgacggcgcc
     1021 gatctcgcgc gtcgacggct tgttgaggct ggcggggtcg cgcatcaggc cctcgaccag
     1081 atggttgagg atcgcgagcg cgatataaac caggagcaag ctcgaaatcg tttcgttgac
     1141 gccgcggtat tgccgaaggc cacccgcaag caggatccaa agtccaccgc cgatcatgcc
     1201 ggcgcaaacc atggcgagct gcacgatgag cggcggtgcc gactgcatca ccaatgcggt
     1261 gctggtcgcc gccagcgcgc cgatcaggag cgcgccttcg ccgccgatga tcaccatgcc
     1321 gagctgcgcg ggcagcgccg tgcacagcgc ggtcagtatc agcggcgccg cgcgcgtcag
     1381 ggtgttctgc caggaaaacc aggtgccgaa cgcgccctgg tacatataga aatagagatc
     1441 gagcgggctt ttgccgaaca gtgcaacaaa gatcccgaac accccgagcg cgccgaccag
     1501 cgccgcgccc ggaatgacga tgtattcgat gattccgccg tagcgctgca gaaatcccgg
     1561 atcggcggcc ggagaaatcg cagctgcttc gaggggatcc gcaacgcctg atgtcacgaa
     1621 gtcgctccga tgacgccctc gacgaggtag tccatcttct cgagttcggg gtccttctgg
     1681 ccgcgatcgg tcccggctga aatcacggtc ttgcccttgt tgtccatgat cgggcccttg
     1741 aagatgacgt aatcgcctga cgtgagcttg gccttgatat cgtcggcgtg cttgcgcgcc
     1801 tccgccgaga ccgcttcgcc gtaaggcgag accttgacga tctcttcttt cagcccgccg
     1861 cgatagaaat tcgggatttc ctgccggccg cgatcatctt gacgaacttc ggatagagcg
     1921 cttcccaatt ccactccgcg ccggtcagat aggccttcgg cgccagcggg gactgattga
     1981 cgtgatagcc gcagaccatc gcgccgcggc gcgccgcgtt ttcgaccatc gtcttcgggc
     2041 cgtcgacgtg gcaggtcaga acgtcgacgc cctgatcgat caggctgttg gtcgcttcgg
     2101 cttccttgac cggcatcgac cagtcaccgg tgaagatcac ctgcgtggtg gctttcgggt
     2161 tggcgagctt tgcgccgagc gtaaaggcgt tgatgttgcg cagcacctgc gggatcggct
     2221 tggcggcaac gaagccgagt ttgttggttt tggtcgagta tccggcgacg atgcctgaaa
     2281 tatattgcgc ctcgtcgatg tagccgaagt aactgccggc gttcttcgga tccttgtcgc
     2341 tccacaagcc gccgcaatgc tcgaagcgga gcttgggaaa cttgtttgcc atcttgatca
     2401 tgtgcggatt gtaataaccg aacgaggtcg ggaacagcag cgttgcgccg tcgagattga
     2461 tcatcgactc catcgtcttt tcgacggcgt cggtttccgc gactttttct tcctcgacga
     2521 tcttgagacc gggcatcttc ttcagcgccg cggcgccctg cgcgt
//
LOCUS       DATNOI010000415         3126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2524003,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000415 DATNOI010000000
VERSION     DATNOI010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2524003"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VN968_16665"
     CDS             complement(<1..825)
                     /locus_tag="VN968_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HXL30900.1"
                     /translation="MSLFSDPRWSEALGHLPDYLGNHVRVSVTALTLGLLVSLPLAIF
                     SRNHPVMRGALLGLASIVQTVPGLALLALFYPLLLALAALSLSWFGISFSAFGFLPAV
                     LALALYSMLPVLRNTITGLHGVEPAILEAARGVGMTPHQSLFMVELPLALPVMMAGIR
                     TAAVWVIGTATLSTPIGQTSLGNYIFAGLQTQNWVFVLFGCLAAAVLALAVDQLLALI
                     ETGLRQRSRLGVAFGGVGIAVLVAATLMPAVTRSQSTYIVGAKTFAEQYVLSALIAQ"
     gene            complement(822..1625)
                     /locus_tag="VN968_16670"
     CDS             complement(822..1625)
                     /locus_tag="VN968_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL30901.1"
                     /translation="MTGAPPLIAFAHVGKSFDGGRGAKPVLAVDDVSLDVAEGELLAI
                     VGSSGSGKTTLLRLASRLIEADSGSITIEGADVRTVDPVQLRRRIGYVFQSGGLFPHM
                     SVADNIGITPKLLGAPAAEIASRVDELLDLVRLDRALHRDRLPEELSGGQRQRVGVAR
                     ALAARPCIVLMDEPFGALDPLTRDALGDDYRALHRKLGLTTVMITHDVTEAILLADRV
                     AVMRAGRLLAQGTPAQLSRSDDPYVGELLRTPRRQAERLNVLLPRGGPA"
     gene            1710..1941
                     /locus_tag="VN968_16675"
                     /pseudo
     CDS             1710..1941
                     /locus_tag="VN968_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442215.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            2863..>3126
                     /locus_tag="VN968_16680"
     CDS             2863..>3126
                     /locus_tag="VN968_16680"
                     /inference="COORDINATES: protein motif:HMM:NF044822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30902.1"
                     /translation="MRKSFRDISTPTLLLTANVSTLFGANLPCLTSLLLHLGSRQAPF
                     TIVSTGTWVILMAPGFSVKGLDPADDTLANVDVEGRPVATARFM"
BASE COUNT          597 a         1027 c          997 g          505 t
ORIGIN      
        1 ctgcgcgatc agcgccgaca gcacatattg ttcggcaaag gtcttggcgc cgacgatata
       61 ggtcgattgc gagcgcgtca ccgcgggcat cagggttgcc gcgaccagca ccgcgattcc
      121 gacgccgccg aacgccaccc ccaggcggct gcgctggcga aggccggtct cgatcagggc
      181 cagcaattga tcgaccgcga gcgccagcac ggcggcggcg aggcagccga acagcacgaa
      241 cacccagttc tgggtctgca atcccgcaaa aatgtagttg ccgaggctgg tctgtccgat
      301 cggggtcgac agcgtcgcag taccgatcac ccacaccgcc gcagtgcgga tgcccgccat
      361 catcaccggc agcgccagcg gcaactcgac catgaacagc gactgatgcg gcgtcatgcc
      421 gaccccccgc gcggcctcga ggatcgcagg ctccacgcca tgcaggccgg tgatggtgtt
      481 gcgcagcacc ggcagcatgg aataaagcgc cagcgccagc accgcgggaa ggaatccgaa
      541 cgcggaaaag ctgatcccga accacgacaa cgacaacgcc gccagcgcca gcagcagcgg
      601 atagaacagc gcgagcagcg cgaggcccgg caccgtctgc acgatgctgg cgaggcccag
      661 cagtgcgccg cgcatcaccg ggtggttgcg cgagaaaatc gccagcggca ggctgaccag
      721 gaggccgagc gtcagcgccg tgacgctgac gcgcacgtga ttgccgaggt aatccggcag
      781 atgccccagc gcctcgctcc agcgcggatc ggagaacaga ctcatgccgg gccacccctc
      841 ggcagcagca cgttgagccg ttcggcctgc cgccgcggcg tgcgcaacaa ctccccgacg
      901 taaggatcgt cacttctgga aagctgtgcc ggcgtgccct gcgccagcag ccggccggca
      961 cgcatgaccg cgacgcggtc ggcgagcaga atggcctcgg tcacgtcgtg ggtgatcatg
     1021 accgtggtca gtccgagctt ccggtgcaag gcgcggtaat cgtcgccgag cgcatcgcgg
     1081 gtgaggggat cgagcgcgcc gaacggctcg tccattagca cgatgcatgg ccttgccgcc
     1141 agggcgcgag cgacgccgac gcgctggcgc tggccgcccg acagttcctc cggcaggcgg
     1201 tcccgatgta gtgcgcgatc gagccgcacc agatcgagca attcatcgac ccgcgacgca
     1261 atctccgccg ccggtgcgcc gagcagcttt ggcgtgatgc cgatattgtc ggcgacgctc
     1321 atatgcggaa acagcccacc actctggaac acgtagccga tgcggcggcg cagctgaaca
     1381 ggatcgaccg tgcggacatc ggcgccttcg atcgtgatgc tcccgctgtc ggcctcgatc
     1441 agccggctgg cgagccgcag cagcgtggtc ttgccggagc cggagctgcc gacgatggcg
     1501 agcaactcgc cctcggcgac gtcgagcgag acgtcgtcca cggccagaac gggtttcgcg
     1561 ccgcggccgc cgtcgaagct tttgccgaca tgcgcaaatg cgatcagtgg cggggcgccg
     1621 gtcatgacgt ttggactatc acaccgcgtc caacccacca agccgacgct ggccttgcct
     1681 attgcgcgat cggttgaatt gttgggatga tggcggtaat ccaccggcca caatgtgggg
     1741 ggaagcgggt gcaggcaggc gagaccgcgg gatcgacggc ggtcgcgctt gacgacgcga
     1801 cggtggcttt cccggtggca gatggccgtg tctacaccgc ggtcgaaaag gcccggctta
     1861 aggattgagc atggcgagtt cgtcgccatc gtcgggccca ccgggtgcgg gaaatcgacg
     1921 cttctcaatg tcgccgccgg ccacgcgtca gcaatcagcg acaaaggccc catagcgatc
     1981 acgaccgcat ccgcctcgac gagggcgcca tcgacctcag caccccttac cgttgatcca
     2041 tccgcagacc atcccggtga cccgtgcgcg gcgctcatga tcgcggaagt gaacttgcgt
     2101 gggtgaacaa tcgccgtcgt ctccggcgtg ccgaatcgat gggcaatcat aactccgtcg
     2161 gaaagccaat cgagcttgga cggcacaggg cgacgcgcgt cgtcatcggg gacaacgaac
     2221 ccgctgtacg cggtcgtgcg ctggtaggcc caatcagcgg taatctcgcg gggcagggcg
     2281 gcatgaagtt ggaagctccc gtcgtgccaa cgcatcgagc ggagtcccgg cacaccagtc
     2341 aagcgccaga aagccgccgg ccttgccgga cgccgcggcg gccacttcgc tccactttac
     2401 cacaatgacg tcgatcccgg ggcaagcaag gaactaggcg gtgcaggctc cgatcacgcc
     2461 gcctccgcaa attacgaccc gcatgatgtc tccaggaaca cctacagaag cgccgagcca
     2521 gcccaggccc ggttcattgg ctatccgctc gcccggccac cagtttgacg gctggttatt
     2581 ggccgaaatc ttggtggcac ctagcgtaaa caggtggctt cgctttggtg cctctctaaa
     2641 taggggcaga cttcgccccg aaggaccgct cctatcagag ggtcgatggc gaggcgcggt
     2701 gccacagaaa cggcgccgcg cccgattagt tcagctccgg cgctcggctt gaaaagcacc
     2761 tataggcggg aattgcacct cgccttttgc ctatctcggc aagttattga aggtgctaac
     2821 atcttctccg ctctccgagc gcaccaaagg cataagccgt tgatgcgaaa aagttttcgc
     2881 gatatttcta ctcccacatt gttactcaca gcgaatgtta gtactttgtt cggtgcaaat
     2941 cttccctgtt tgacctcgct gctcctgcat ctcggctcgc ggcaggcgcc attcacgatt
     3001 gtttcgaccg gcacctgggt gatcctgatg gcgccaggct tcagcgtgaa ggggctcgat
     3061 ccggctgacg acacgctggc caatgtcgat gttgagggac gtccggtcgc caccgcgcgt
     3121 ttcatg
//
LOCUS       DATNOI010000416         3095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1100542,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000416 DATNOI010000000
VERSION     DATNOI010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100542"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VN968_16685"
     CDS             complement(<1..201)
                     /locus_tag="VN968_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HXL30903.1"
                     /translation="MSELPQKDPAPADDAAEGEPRRGRVRKPIGWSMIAIGVLVAVSA
                     TLVWRRDGIGGVLDILTHDVTLF"
     gene            complement(312..686)
                     /gene="crcB"
                     /locus_tag="VN968_16690"
     CDS             complement(312..686)
                     /gene="crcB"
                     /locus_tag="VN968_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HXL30904.1"
                     /translation="MHYLLVFIGGGLGSTLRHLVNVVTPRLLGTAFPYHTFIVNISGS
                     IVMGLIAGYLAFRGEASQPWRLFLMTGVLGGYTTFSAFSLDAVLLYERGAIGLALFYV
                     LGSVAFSIAGLFASLALVRHFT"
     gene            complement(857..1453)
                     /locus_tag="VN968_16695"
     CDS             complement(857..1453)
                     /locus_tag="VN968_16695"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HXL30905.1"
                     /translation="MTFTLPTLPYAYDALQPHMSKETLEYHHDKHHQAYVTNGNNLLK
                     GTEFEGKSLEEIVKGSFGKNAPLFNNAGQHYNHLHFWNWMKPNGGGNKLPGRLEKKIT
                     EDLGGFEKFKTDFAAAGVGQFGSGWAWLSVKNGKLEISKTPNGENPLVHGATPILGVD
                     VWEHSYYIDYRNRRPDYLKAWIDHLVNWEYVDQLFSKA"
     gene            1616..2143
                     /locus_tag="VN968_16700"
     CDS             1616..2143
                     /locus_tag="VN968_16700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL30906.1"
                     /translation="MSIEIEVLNGEASWPIAKPLFEAVWPSDVVQKLPWGHVKWAHAD
                     LRVMIETPSDGLACHVGVYFRNVTWNGRKLHIGGVGGVLTRADCRRRGYASIALDAAI
                     QTMRDREDVQFALLFCEPHNFAFYQSRGWHPFAGEVYAEQPDGKIRFEAMAPFVHDIK
                     RAPRQGVIDLCGLPW"
     gene            2232..2675
                     /locus_tag="VN968_16705"
     CDS             2232..2675
                     /locus_tag="VN968_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HXL30907.1"
                     /translation="MACRFGFIIAILTAWFFAAPANAQSGGEASGIWLTQAGDAKVRV
                     SKCGGGICGVVVWLKDPINPATGKPHVDDKNPNPSLARRPMIGLPLFVGMHPSGPDRW
                     SGQIYNADDGNSYVSNISVPGPDTLRVEGCVGMLCGGETWTRSGR"
     gene            complement(2757..>3095)
                     /locus_tag="VN968_16710"
     CDS             complement(2757..>3095)
                     /locus_tag="VN968_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3095 family protein"
                     /protein_id="HXL30908.1"
                     /translation="SVGGFVPKTYVQQVVENSDFRKYDDGLRMILDCTPELENALTQR
                     LAVAASKGIARYGLHRQDAAMMTCFTPSALRSDHVHFIDGARGGYGALLIRGPFGTSH
                     RLGSRLCGAS"
BASE COUNT          638 a          925 c          953 g          579 t
ORIGIN      
        1 gaacagcgtg acatcgtggg tcaggatatc aagtacgccg ccgatgccgt cgcggcgcca
       61 gaccagcgta gcgctgaccg cgaccaagac gccgatcgcg atcatcgacc agccgatcgg
      121 cttgcgcacc cggccgcgac gcggctcgcc ttctgcggcg tcgtcggcag gcgccggatc
      181 tttctggggg agttctgaca aggcaaatga tctggcgcgc tgtggagcta aattggctta
      241 atctcggcgg cacagcgatg caaacataag cgtgacgaga cccatgcgtg tttcacggat
      301 gcccatgcgg ctcacgtgaa atggcgcacc agcgccaggc tcgcgaacag gcctgcaatc
      361 gaaaacgcca ccgaacccag cacatagaac agcgcaagcc cgatcgcgcc gcgttcgtag
      421 agcagcacag catcgagcga gaacgccgag aaggtggtgt agccgccgag cacgccggtc
      481 atcaagaaca gccgccacgg ctgcgaggct tcgcccctga aggcgagata gcccgcgatc
      541 aggcccatga cgatcgagcc tgatatgttg acgatgaagg tgtgatacgg aaaggccgtt
      601 ccgagcaagc gcggggtgac gacgttgacg aggtgacgca gcgtcgagcc aagaccgccg
      661 ccgataaaca ccaggagata atgcatcgcg agttttcccc caccggcaga atgagaccac
      721 cggcactgca caggttttgc cgcaagcccg cgatccccgc aaggatcgcc aggcggcatc
      781 ggcaaacgaa aaggcggcag ccaggatgcg ctgccgcctc aagatcgtcg cctggcaaaa
      841 ttcgccgttg ctccggtcag gctttcgaga acagctggtc gacatactcc cagttcacca
      901 gatgatcgat ccacgccttc agataatccg gacggcgatt gcgataatcg atgtagtagg
      961 agtgctccca gacgtcgacg ccgaggatcg gggtggcgcc atgaaccagc gggttctcgc
     1021 cgttcggggt cttcgagatc tcgagcttgc cgttcttcac cgacaaccac gcccaacccg
     1081 aaccgaactg gccgacaccg gcggcggcga aatcggtctt gaatttctcg aacccgccaa
     1141 ggtcctcggt gatcttcttt tccaggcggc ccggtaattt attgccgccg ccattgggct
     1201 tcatccagtt ccagaaatgc aaatggttgt agtgctggcc cgcattgttg aagagcggcg
     1261 cattcttgcc gaacgagcct ttgacgatct cttcgaggga cttgccctcg aattcggtgc
     1321 ctttcagcag attgttgccg ttggtcacgt aggcctgatg atgcttgtcg tgatgatatt
     1381 ccagtgtttc cttggacata tggggctgca acgcatcata ggcgtaagga agggttggaa
     1441 gcgtgaaggt catggggtga tgtccgaaaa tgatgggaga cgtggcttaa cggcgaccct
     1501 tatagaaggt tccatcggtc ttaaacactt gaatttggga aaacggcggg tgtgacggcc
     1561 caaggttttc acggaggcgt atgacaacag ggctgcgcgc tggagaaaaa gcggaatgag
     1621 catcgagatc gaggttttga acggcgaggc atcgtggccc atcgcaaaac cgctgttcga
     1681 agcggtgtgg ccgtccgatg tggtacagaa gctgccttgg ggccacgtca agtgggccca
     1741 tgcggacctc cgcgtgatga tcgaaacgcc ctcggacggc cttgcctgtc acgtcggcgt
     1801 ttacttccgc aacgtaacct ggaacggacg caaacttcac atcggcggcg tcgggggtgt
     1861 cttgacccgc gcggactgcc gccgccgcgg ctatgccagc atcgcactcg acgcggcgat
     1921 ccagaccatg cgcgatcgcg aagacgtcca gtttgcgctc ctgttctgtg agccgcacaa
     1981 ttttgccttc taccaatccc gcggctggca tccttttgcc ggtgaagtct atgccgaaca
     2041 acccgatgga aaaattcgct tcgaggcgat ggcgcccttt gtccacgaca tcaagcgcgc
     2101 gccgcggcag ggcgtcatcg acctctgcgg cctgccctgg tgatcatgcg ccaagtccat
     2161 gatccgctgt ggatacagca ttgcacgaat ccggtttgct gctatgcagg cgtgcttttt
     2221 tgaggtgccc catggcttgc cgattcggct tcatcattgc gatcttgacg gcatggtttt
     2281 tcgcagcgcc agcgaatgcg cagagcggtg gagaggcgag cggcatctgg ctgacgcagg
     2341 cgggcgacgc gaaagtgcgc gtcagtaaat gcggcggagg aatttgcggc gtcgtggtct
     2401 ggctgaagga cccgatcaac cccgccaccg gaaaacctca cgtcgatgac aagaatccca
     2461 atccctccct cgccagacgt ccgatgatcg ggcttccgct atttgtcggc atgcaccctt
     2521 caggcccgga cagatggtcg ggccagatct acaacgcgga tgacggcaac agctatgtca
     2581 gcaacatctc ggtgccgggt ccggatacct tgagagtcga aggctgcgtt ggcatgctct
     2641 gcggcggcga aacctggacg cgctccgggc gctgaaagcg tccactaaga gcgcctcctc
     2701 accatgagag cgtgtgaaac aatgcgtccc gtgtcccgga cgcggtgcag cgttcttcac
     2761 gatgcaccgc agagccggga cccaaggcga tgcgaagtgc cgaatggacc ccggatcagc
     2821 agcgcaccgt aaccgccgcg cgcgccgtcg atgaaatgca cgtgatcgct gcgcagcgcc
     2881 gaaggcgtaa agcaggtcat catcgccgcg tcctgccggt gcaggccgta tcgcgcgatt
     2941 cccttggagg cggccacggc gaggcgctgc gtcagcgcgt tctccagctc cggcgtgcaa
     3001 tcgaggatca tgcgcaatcc gtcgtcgtat ttgcggaagt cggaattctc caccacctgc
     3061 tgcacatagg tcttcggcac gaagccgccg acgct
//
LOCUS       DATNOI010000417         6221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_545165,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000417 DATNOI010000000
VERSION     DATNOI010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545165"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1673
                     /locus_tag="VN968_16715"
     CDS             129..1673
                     /locus_tag="VN968_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30909.1"
                     /translation="MVSSALSRRYVLQLAGAAALPLSSGLLGGPAQAAATGTLTIAYN
                     VNLPSFDPTIGLSAVNPTLQSIYQAIFDPYIGQAPDLSFKPGLLTKWGWNADHTKITL
                     ELRKGVTWHDGSPVTPEDVVWSLQRAADPKSGNPIQFVWSKVGNYKIDGNTITGDVIE
                     FEPTLFKWMAFLTGYILPKAYYEKVGAEGFEKAPIGSGPYKVDAFERNAFLRLKTHPT
                     YWGPKPAFDTVVFKFVTDPTSRVAEMESGGSDVTFEIPYEEFDRLKAKAGLAGQTKPV
                     SDIAMIFITDIAPMLDRNVRLAANHAIDKKAIVDRLLKGYGVPIATLEAPGYAAFDPS
                     IKVSYDPELAKSLLAKSGFSPEKPVKFTIQTTRGFKPKDYEMIQAIVGMWRKVGIEAN
                     IEVYEIAQHFELRARHALAPAAFYNWGNAIGDPSTSTGFAMFGPSPHCAWKGKDLVER
                     IGPLWGEKDEAKRIAGWKAVDKYIAEEGEVIPLFQYVQPIIHKKGLKVVAQANGMILP
                     QLITQA"
     gene            1699..2706
                     /locus_tag="VN968_16720"
     CDS             1699..2706
                     /locus_tag="VN968_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL30910.1"
                     /translation="MRWGEIIRRLVLIPPTLVGVAVIVFVLLRVVPGDPIAMMIPPGA
                     SPADIDRLRAFYGLDQPILQQFITWFGQALRGDFGRSISVHQSVFDLVLARLPATLEL
                     AALATLIAVVLGTIAAMIGIQLRGRRAEWVVDGGVGVFLAIPDFLWALILLLLFGVLI
                     PLLPISGRIDPQIDVNFHSNFYLVESFLTGRFDIANALLHHMLLPALALALPFAALVA
                     RILKTSLAEAEDQDYAQIARARGFSRSAILLREVFPNALIPTVALGGVQITLLLGGTV
                     LVERIFSYEGIGNMAIDAVINRDFPLIQGLILTFAVLFIALNLGVDLVVTLLDPRLRH
                     G"
     gene            2699..3586
                     /locus_tag="VN968_16725"
     CDS             2699..3586
                     /locus_tag="VN968_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL30911.1"
                     /translation="MVDLALDSALGPPSRWIAGLKRGGPRLWVGAALVGVLVLAALLA
                     PLIALHDPLEQDLMSAQLPPAWVVGGDLAYWLGTDSLGRCVLSRLIYSARTAVAVALI
                     AASLAAAIGIAFGLIAGSFGGWVDQATSRLIDVWMAFPPVLLSIVLAAVIGAGLTSVI
                     AAIVVVDWTRFARVVRAETMVQLQRDYAAAARVIGLSRSGILRLEILPNLIPLVVTLL
                     AVEMGIAILVEVILSFVGISVAGDTPTWGGMIAEGRQIVYQAPWIMALPIGCIISSVI
                     GFNLLGDGLRLVLDPVQRR"
     gene            3583..4470
                     /locus_tag="VN968_16730"
     CDS             3583..4470
                     /locus_tag="VN968_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30912.1"
                     /translation="MTAALSVQGLQVAIGTGASSANVLRGVSLDLQRGEVRGLVGESG
                     AGKSMLGRAVLGLLPANAVIAAGTIQFEGRDLVAMPEPERRSLLGRRIALIPQDPMTS
                     LNPVKRVGAQIAILLRHHLALSKRGALERAAELLAEVAIREPRRVLELYPHEISGGMR
                     QRVLIAMAFGCDPSLVIADEPTTALDVTVQRQVLQLVERLRQRHGAAVLFITHDLGVV
                     AKICRTVTVLHAGRVLEEGETAEVLAKPRHPYTRALLAATPRADRPAEALRPVPPALI
                     ENLWSEAHRLDRLVVAGPA"
     gene            4467..5324
                     /locus_tag="VN968_16735"
     CDS             4467..5324
                     /locus_tag="VN968_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL30913.1"
                     /translation="MTDILLRTGSLSFAYPERAGFLRRASLREVVKRVDLAVPRGSVL
                     GLVGESGSGKTTLGRLLVRLLRPTSGGIQFDGAEIGTLEEAEMRPLRARIQMIFQDPQ
                     SSLNPRLRLGVTLTRPLQVYGRLKGRRDQRDRAAALLDLVGLPTGFVDRYPHELSGGQ
                     RQRAGIARALALDPDFIVADEIVSGLDVSTQPYILLLLKELCARLRLTMVFISHDLSV
                     VRVLCDDVAILRHGEVVEYGPVARIFGFPQHAYTRQLLSAIPLPDVEPGWLDDPSPIV
                     PSHQQGPAA"
     gene            5321..6040
                     /locus_tag="VN968_16740"
     CDS             5321..6040
                     /locus_tag="VN968_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL30914.1"
                     /translation="MTETIKGSVVLITGATGAIAQALIAGLAARGAAKIYAAARDISF
                     LAASPQLVPIKMDVTSDEDVAKAAAIATDVTLLINNAGVNHNTSFMTAPDMAIAREEI
                     EANYLAPLRVTRAFAPALTANRGAVLNLLTILARVNLPFMGSYCASKAAALSLTQGLR
                     GELVPKGVRVAAALPGAIDTRMTAVLTIPKMTTTEAAAEILDGFEAGEEDIYVGDMAR
                     GIAQGLAHDPKAVERQLASPG"
     gene            6088..>6221
                     /locus_tag="VN968_16745"
     CDS             6088..>6221
                     /locus_tag="VN968_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HXL30915.1"
                     /translation="MTHHRILLAFAALALGVSPMPVSADFIPGLRGHDHTGITVPDVK
                     A"
BASE COUNT         1021 a         1982 c         2008 g         1210 t
ORIGIN      
        1 cgacgagacg ctaccgtcga acacgctgat ggcgcgttgg gcagaaagtg cgccgcaggc
       61 cgccgaatag tcgcattcag ccaatggcgg ccgctccggg cgccattctc gcatctggag
      121 taattgtcat ggtgtcctcc gcgctttctc gccgctacgt tcttcagctg gccggcgccg
      181 ccgccctgcc gctctcctcc ggtctcctcg gtggccccgc gcaggccgca gccacgggaa
      241 cgctgaccat cgcctacaat gtcaatttgc cgtcgttcga tccgaccatt ggattgtccg
      301 cggtcaatcc gaccctgcag tcgatctatc aggcgatctt cgatccttac atcggtcagg
      361 cgcccgacct ttccttcaag cctggtctct tgacgaaatg gggctggaac gccgaccaca
      421 ccaagatcac gctggagctg cgcaagggtg tgacctggca cgacggctcg ccggtgacgc
      481 ccgaagacgt ggtctggtcg ctgcagcgcg cagccgatcc gaaaagcggc aatccgattc
      541 agttcgtctg gtccaaggtc ggcaattaca agatcgacgg caacaccatc accggcgacg
      601 tcattgaatt cgaaccgacc ttgttcaaat ggatggcttt tctcaccggc tatattctgc
      661 cgaaggccta ttacgagaag gtcggtgcgg aaggttttga gaaggcgccg attggctccg
      721 gtccctacaa ggtcgatgcc ttcgaacgca atgccttcct gcggctcaag acgcatccga
      781 cctattgggg acctaagcct gccttcgaca ccgtggtgtt caaattcgtc accgatccca
      841 ccagccgcgt cgccgagatg gaatcgggcg ggtcggacgt cacgttcgaa attccctacg
      901 aggaattcga tcgcctgaag gcgaaagcgg ggctggccgg acaaaccaag ccggtgtccg
      961 acatcgcgat gatcttcatc accgacatcg cgccgatgct cgaccgcaac gtccgtctcg
     1021 ccgccaatca cgcgatcgac aagaaggcga tcgtcgaccg gctgctgaag gggtacggag
     1081 ttccgatcgc gacgctggaa gcgccgggct acgccgcctt cgatccctcg atcaaggtgt
     1141 cttacgaccc cgaactggca aaaagcctgc ttgccaagag cggcttctcg ccggagaagc
     1201 cggttaagtt cacgatccag acgacgcgcg ggttcaaacc gaaggattac gagatgatcc
     1261 aggcgatcgt cgggatgtgg cgcaaggtcg ggatcgaggc caatatcgag gtctacgaaa
     1321 tcgcgcagca tttcgagctg cgcgcgcgcc acgcgctggc gccggcggcg ttctacaatt
     1381 ggggcaacgc gatcggcgat ccctccacct ccaccggctt tgcgatgttc ggcccgtcgc
     1441 cgcattgcgc ctggaagggc aaggatctcg tcgagcgcat cggcccgctg tggggcgaaa
     1501 aggacgaagc caagcggatc gccggctgga aggctgtcga caaatacatc gcggaggagg
     1561 gcgaggtgat cccgctgttc cagtatgtgc agccgatcat ccacaaaaag ggcctgaagg
     1621 tcgtggcgca ggccaatggt atgattcttc cgcaactgat cacacaggct tagggacgca
     1681 agatagggcg acagcacgat gcgatggggc gagatcatca ggcgactcgt cctcataccg
     1741 cccacgctgg tcggcgtggc ggtgatcgtc ttcgtgttgc tgcgcgtggt gcccggcgac
     1801 cccatcgcca tgatgatccc gccgggcgct tcgcccgccg acatcgaccg gctgcgggcg
     1861 ttttatggac ttgatcagcc catcctgcag cagttcatca cctggttcgg ccaagcgctg
     1921 cgcggcgatt tcggacgttc aatcagcgtg caccagagtg tgttcgacct ggtgctggcg
     1981 cgattgccgg ctacgctcga attggcggcg ctggcgacgc tgatcgcggt ggtgctggga
     2041 acgatcgccg cgatgatcgg catccagctt cgcggccggc gggcggaatg ggtggtcgac
     2101 ggtggcgtcg gtgtcttcct ggccattccg gattttctct gggccctgat cctgctgctg
     2161 ctgttcgggg tgctgatccc gctcttgccg atttcgggcc gaatcgaccc gcagatcgac
     2221 gtcaacttcc acagcaattt ctatctggtc gaaagctttt tgaccgggcg cttcgacatc
     2281 gccaacgcgc tgctgcatca catgttattg ccggcgctgg cgctggctct accatttgcg
     2341 gcattagtgg cgcggatttt gaaaacctcg ctggcggagg ccgaggatca ggattacgcg
     2401 cagatcgccc gcgcccgcgg gttttcgcgt tcggcgatcc tgctgcgcga ggtatttccc
     2461 aacgctctga ttccgacggt tgcactcggc ggggtccaga tcacgctgtt gctcggcggc
     2521 accgtgctgg tcgagcgcat cttctcctat gagggaatcg gcaacatggc gatcgacgcg
     2581 gtgatcaatc gcgatttccc gctgatccag ggcctgatcc tgaccttcgc cgttctcttc
     2641 atcgcgctca atctcggcgt cgatctcgtg gtcacgctgc tcgatccgag gttgcgccat
     2701 ggttgacctt gccctcgata gcgcgctcgg gcctccgtcg cgctggatcg ccggattaaa
     2761 gcggggcggg cctcggctct gggtcggtgc ggcgcttgtc ggcgtcctcg tactggccgc
     2821 gctgctggcg ccgctaatcg cgctgcacga tccactcgaa caggatctga tgtcggcgca
     2881 attgccgccg gcctgggtcg tcggcggtga cctcgcgtat tggctcggca ccgacagtct
     2941 tggccgctgc gtgttgtcgc gcttgattta ctcggcgcgg accgccgttg cggtcgcgct
     3001 gatcgcggca tcgctggcag ccgcaatcgg catcgccttt ggcctgatcg ccggcagttt
     3061 cggcggctgg gtcgatcaag ccacttcgcg cctgatcgat gtctggatgg catttccgcc
     3121 ggtgttgctg tcgatcgtgc tggcggcggt gatcggcgcc gggctgacct cggtgatcgc
     3181 tgccatcgtg gtcgtcgact ggacccgctt tgcgcgcgtg gtccgggccg aaaccatggt
     3241 gcaactgcag cgcgattacg ccgctgccgc gcgcgtcatt ggactgagcc gcagcggaat
     3301 cctgcggctg gagattctgc ccaacctcat tccgctggtg gtgacgttgc tggcggtcga
     3361 gatgggcatc gcgatcctgg tagaagtgat cctgtctttt gtcggcattt cggtggccgg
     3421 cgacaccccg acctggggcg gcatgatcgc cgagggacgc cagatcgtct atcaggcgcc
     3481 atggatcatg gcgctgccga tcggctgcat catatcaagc gtgatcgggt tcaatctgct
     3541 cggcgacggc ctgcgtttgg tgctcgatcc ggtgcagcga cgatgacagc agcgctttcc
     3601 gtccagggcc tgcaggtcgc gattggcacg ggcgcaagct cggccaacgt gctgcgtggc
     3661 gttagcctgg acctgcagcg cggcgaagtc cgcggattgg tcggggaatc cggcgccggc
     3721 aaatcgatgc tgggacgtgc cgtcctcggc ttgctgccag cgaacgcggt tatcgctgcc
     3781 gggaccattc aattcgaggg acgcgatctc gttgccatgc cggagccgga gcgacgcagc
     3841 ctgctcggcc gccgcatcgc gctgattccg caggacccga tgacctcgct caatccggtg
     3901 aagcgtgtcg gcgcgcagat cgcgatcctg ctgcggcacc accttgccct gtcgaaacgc
     3961 ggcgcgctgg agcgggcggc tgaactgctt gccgaggtcg cgatccgcga gccgcggcgg
     4021 gtgctggagc tgtatcccca cgaaatctcc ggcgggatgc ggcagcgcgt gctgatcgcg
     4081 atggcgttcg gctgcgatcc gagcctggtg atagcggatg agccgaccac cgcgcttgac
     4141 gtcactgtgc agcgccaggt gctgcagctg gtcgaacgtc tccggcagcg ccatggtgcc
     4201 gcggttttgt tcattaccca cgacctcggc gtcgtcgcca agatatgccg taccgtgacc
     4261 gtgctgcatg cggggcgcgt gcttgaggaa ggcgagacag ccgaggtgct ggcaaagccg
     4321 cgtcatccct atacgcgcgc gctgctggcg gcgacgccgc gcgccgaccg gccggctgaa
     4381 gcgctgcgcc cggtaccgcc ggctctgatc gagaacctgt ggtcggaggc gcatcggctc
     4441 gaccggttgg tagtagcggg gccggcatga ccgatatcct gctccgaacc ggcagcctca
     4501 gttttgccta tccggagcgc gccgggtttt tgcgccgggc gtcgttgcgc gaggtggtca
     4561 agcgggtcga tctcgcggtg ccgcgcggtt cggtgctcgg tcttgtcggc gagtccggtt
     4621 ccggcaagac caccctgggc cggctcctgg tgcggctttt gaggcctacc tccggcggca
     4681 tccagttcga cggcgccgaa attggaacgc tcgaagaggc ggagatgcgg ccgctgcggg
     4741 cgcgcatcca gatgattttc caggatccgc aatcatcgct gaatccccga ctgcgactgg
     4801 gcgtcacgct gacgcgtccg ctgcaagtgt acgggcgctt gaagggacgg cgggaccagc
     4861 gtgatcgcgc cgcagccctg ctcgatctgg ttggattgcc taccggcttc gtcgaccgct
     4921 atccgcacga gctttcggga ggccaacgcc agcgggcggg gatagcccgc gctttggcgc
     4981 tggatccgga cttcatcgtc gccgacgaga tcgtgtccgg cctcgacgtg tcgacgcagc
     5041 cttacatcct gctgctgctc aaggagctgt gcgccaggct tcgtctcacc atggtgttca
     5101 tcagccacga tctgtctgtg gtccgcgtgc tctgcgacga cgtcgcgatc ttgcgccatg
     5161 gcgaggtcgt cgaatacgga ccggtcgccc ggatcttcgg cttcccgcag catgcctaca
     5221 cgcgacagct gctctctgcg atcccattac ccgacgtcga gccgggctgg ttagacgatc
     5281 ccagcccgat tgtgccttcc caccagcaag gaccagccgc atgaccgaga ccatcaaggg
     5341 ttctgtcgtt ctcatcacgg gggccaccgg cgcgatcgcg caggcgctca tcgccgggct
     5401 tgcggcccgc ggtgccgcca agatttacgc cgccgcgcgg gacatttcct tcctcgccgc
     5461 atcgccccaa ctggtgccga tcaagatgga cgtcaccagc gacgaggacg tcgcgaaagc
     5521 cgccgcgatt gcgaccgatg tcacgctctt gatcaacaat gccggcgtga accacaacac
     5581 aagcttcatg accgctccgg acatggcgat tgcccgcgag gaaatcgagg ccaattatct
     5641 ggcgccgttg cgggtgacgc gcgccttcgc gccggcgctg accgccaatc gcggcgccgt
     5701 gctcaatttg ctgacgatcc tggcgcgcgt caacctgccg ttcatgggat cctactgcgc
     5761 gtccaaggcg gcggcgttga gtctgacgca gggactgcgc ggggaacttg tgccgaaggg
     5821 agtccgcgtc gccgcagcgc tgcccggcgc gatcgacacg cgaatgactg cggtgctgac
     5881 gattccgaaa atgacgacga ccgaggccgc ggcagagatc ctcgacgggt tcgaggccgg
     5941 cgaggaggat atctatgttg gcgacatggc gcgcggcatc gcccaaggtc tcgcccatga
     6001 cccaaaggct gtcgaacgcc aattggcgtc gccgggctga tcgactacgg ctgaaagctt
     6061 gaaaatcgca aaccaaagga agcgaccatg acacaccacc gaatcttgct cgccttcgcc
     6121 gcgctggctc tgggcgtatc gccgatgccg gtatcggccg atttcattcc cggcctgcgc
     6181 ggccacgacc acaccggcat caccgtgccg gatgttaaag c
//
LOCUS       DATNOI010000418         3342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1433570,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000418 DATNOI010000000
VERSION     DATNOI010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433570"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VN968_16750"
     CDS             complement(<1..1187)
                     /locus_tag="VN968_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL30916.1"
                     /translation="MPQRWPIIVFALVMAAIPFIPGLPPFWIVLLDNIGLSALVAMGL
                     VLLTGVGGLTSFGQAAFCGFGAYTTAVLTTAYGVSPWLTLPLSLLVSGIAAVALGLVT
                     VRLSGHYLPLGTLAWGLGLFYLFSKLEFLGRNDGISGIPPLSIGSFRMIDPGAIYFAI
                     WIAVLVCASLTTNLLDSRTGRAIRALRRGHIAAEAFGVQAPRAKLLVFIYAAVLAGLS
                     GWLYAHFQRAANPTPFGAQAGIEYLFIAVVGGAGYVWGGVLGAAIVVILKEILQSYLP
                     YLFHGEGQLETIVFGILLVGLLQLAPSGVWPWLTARLPLKSNRKRPDTSLTLPASPKA
                     PAAAGVLLRIEQARKRFGGVIAVNNVSFDVKTREIVALIGPNGAGKSTTFNLITGVLT
                     SSGG"
     gene            complement(1193..2233)
                     /locus_tag="VN968_16755"
     CDS             complement(1193..2233)
                     /locus_tag="VN968_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL30917.1"
                     /translation="MNTTIMLFLVQDGITNGAIYALLGLALVLVFAVTRVILIPQGEF
                     VTYGALTYASLSAGHVPGTARLALAMGVVAFGLDLFAGRRSLHPRLVGRAFAVNVFLP
                     AVIFALTFWLAGRHPPIAVNIALSLLIVAAIGLFLYRIAFQPLAHTSVLVLLIASVGC
                     HLALQGFGLLFFGAEGQRGPTIADTAFRVGPLRFTGQSIAVYAITIAFIVGLWLFFGF
                     TLYGKALRATAVNRLGARLVGIRTTLSGQIAFLLASVIGAISGILIVPITTLYYDTGF
                     LIGLKGFIAAIIGGLVSYPLTAVAALLVGSVEAFSSFYASNFKEVIVFTLIIPVLLLR
                     SLAAPAVEEEKD"
     gene            2408..2929
                     /locus_tag="VN968_16760"
     CDS             2408..2929
                     /locus_tag="VN968_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXL30918.1"
                     /translation="MTISKAAPINAAKVSGPGRKEPAEPATESTALQLGELSEHLGYA
                     LKRAQLKIFEDFLRCVAPLQLTPAQFSVLLLLDKNPGRNQTEIANTLGILRPNFVAML
                     DGLESRDLCARIRSTNDRRSHILVLTDKGRAVLARAKKLVAAKHEARLNELLGPANRA
                     ALLSMLATIAKEF"
     gene            complement(3100..>3342)
                     /locus_tag="VN968_16765"
     CDS             complement(3100..>3342)
                     /locus_tag="VN968_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXL30919.1"
                     /translation="TENTGSLNIVRAVAALANGMGMTATAEGVETSEQLDKITSEGCT
                     EMQGYLFSKPLPAAEIERLFLSGREPRKAPGRIVAA"
BASE COUNT          691 a         1053 c         1087 g          511 t
ORIGIN      
        1 ccgcccgacg aggtgagcac gccggtgatc aggttgaagg tcgtgctctt gccggcgccg
       61 ttggggccga tcagcgccac gatctcgcgg gttttgacgt cgaaggagac gttgttgacg
      121 gcgatcacgc cgccgaaccg cttgcgtgcc tgctcgatcc gcagcagcac gccggcagcg
      181 gccggcgctt tggggctcgc gggcaaggtc agggaagtgt cgggccgctt gcgatttgat
      241 ttgagcggca gccgcgccgt cagccacggc cagacccctg atggcgccag ttgcagcagg
      301 cccaccagca ggatgccgaa cacgatggtc tcgagctggc cctcgccgtg aaacagatag
      361 ggcaggtagc tctgcagaat ttccttcagg atgacgacga tggcggcccc cagcacgccg
      421 ccccacacat agccggcgcc gccgaccaca gcgatgaaga gatattcgat gccggcctgg
      481 gcgccgaacg gcgtcggatt ggcggcgcgc tggaaatgcg catagagcca cccggacagg
      541 ccggccagca ccgccgcata gatgaaaacc agaagtttgg cgcggggcgc ttgcacgccg
      601 aaggcttcgg cggcgatgtg tccgcgccgc aacgcgcgga tcgcgcggcc ggtgcgggag
      661 tccaggaggt tcgtcgtgag cgacgcgcag accagcaccg cgatccagat cgcgaagtag
      721 atcgcgccgg ggtcgatcat ccggaatgag ccgatcgaaa gcggcggaat gccggaaatg
      781 ccgtcgttgc ggccgaggaa ttcgagcttg ctgaacagat agaacagccc aagcccccag
      841 gcgagggtgc caagcggcag ataatggccg gacagccgca ccgtgacgag gccgagtgcg
      901 accgcggcga tgccggagac cagaagcgaa agcggcaatg tcagccacgg cgacacgccg
      961 tacgccgtgg tcagcaccgc cgtggtataa gccccaaagc cgcaaaacgc cgcctggccg
     1021 aacgaggtga ggccgccgac gccggtcagc agcacgaggc ccatcgccac cagcgccgac
     1081 agaccgatat tgtccaacag cacgatccag aacggcggca ggcccgggat gaacgggatc
     1141 gccgccatca ctagcgcgaa tacgatgatg ggccaccgct gcggcattgc ggtcaatcct
     1201 tctcttcttc caccgcggga gctgccagcg agcgcagcag cagaaccgga atgatcagcg
     1261 tgaagacgat gacttccttg aaattgctgg catagaacga ggagaaggct tcgacggatc
     1321 cgaccagcag cgccgcgacc gccgtcaggg gatagctgac gagcccgccg atgatcgcgg
     1381 caatgaaacc tttcaggccg atcaggaatc cggtgtcgta atacagcgtc gtgatcggca
     1441 cgatcaggat tcccgaaatc gccccgatca ccgaggccag caggaaagcg atctgtcccg
     1501 acagcgtcgt cctgatgccg accaggcgcg cgccgaggcg gttgacggcg gtggcgcgca
     1561 gcgccttgcc gtaaagcgtg aagccgaaaa acagccacag cccgacaatg aacgcgatgg
     1621 tgatggcgta gacggcgatg ctctggccgg tgaagcgcag cgggccgacg cgaaaggcgg
     1681 tatctgcaat cgtcgggccg cgctggccct ccgcgccgaa aaacagcagg ccaaatcctt
     1741 gcaaggccag gtggcagccg acggacgcga tcaacagcac cagcaccgag gtatgcgcaa
     1801 gcggttgaaa cgcgatgcga tagagaaaca gtccgatcgc cgccacgatc agaagcgaca
     1861 gcgcgatgtt gacggcgatc ggcggatggc ggccggcgag ccagaaggtc agtgcgaaaa
     1921 tgaccgcagg caggaagaca ttgaccgcga acgcccgtcc gacaagcctt ggatgcagcg
     1981 atctgcggcc ggcgaacagg tcgaggccga acgccacgac ccccatcgcc agcgccagcc
     2041 gggcggtgcc cggcacatgg cccgccgaca gcgaagcata ggtcagcgcg ccgtaggtga
     2101 cgaattcgcc ttgcggaatc aggatgacgc gggtaacggc gaacaccaga accagcgcaa
     2161 ggccgagcag cgcatagatc gcgccgttgg taatgccgtc ctggaccagg aacagcatga
     2221 tcgtggtgtt caaggccggg cgctccctta tatcgggccg atcgcggccc gaaagccgtc
     2281 cgaccgtcca ttttgacagt tgaaaatttc cgccagtatg tatacactat aacagttatg
     2341 aattataatc aagatcggcc gacttcggct gcgcttttca gcctaccggg attgcgagca
     2401 ccgagcgatg accatttcca aagccgcccc catcaatgcc gccaaggtgt ccggcccggg
     2461 ccgcaaggag cctgccgagc ctgccacgga aagcaccgcg ctgcagctcg gtgaattgtc
     2521 cgagcatctg ggttatgcgc tcaagcgcgc gcaactgaaa atctttgagg attttctgcg
     2581 ctgcgtcgcg ccgctgcaac tgaccccggc gcagttctcc gtgctgctgc tgctcgacaa
     2641 gaatccgggg cgcaaccaga ccgagatcgc caatacgctc ggcatcttgc ggccgaattt
     2701 cgtggcgatg ctcgatggcc ttgaaagccg cgatctgtgc gcgcggattc gttcgaccaa
     2761 cgatcggcgc tcccacattc ttgtcctgac cgacaagggc cgggcggtgc tggcgcgcgc
     2821 caagaagctg gtcgccgcca agcacgaagc gcgtcttaac gagctcttgg gtcccgccaa
     2881 ccgcgccgcg ctgctttcga tgctggccac gatcgcaaaa gagttttgag ttctgtgagt
     2941 ctagaaaggc gacgctcccc ggatgctgcg cagcgcgccg cgcttgcggc gtggtgcgct
     3001 gctgatccgg ggtccataat cacacgaggc gcctatgatg ggtcccggat ctgcggagca
     3061 gcgtgaagaa cgctgcaccg cgtccgggac acggaccgat caggccgcga cgatgcggcc
     3121 gggagccttt cgcggctcgc gccctgacag gaatagccgc tcgatctccg ctgcgggcaa
     3181 cggcttactg aacaggtatc cctgcatctc ggtgcagcct tcagatgtaa tcttgtcgag
     3241 ctgttctgag gtttcgacgc cttcggcggt cgccgtcatg cccattccat tcgcgagcgc
     3301 cgcgaccgcc cgcacgatat tgagcgagcc ggtgttttcc gt
//
LOCUS       DATNOI010000419        11960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_540099,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000419 DATNOI010000000
VERSION     DATNOI010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11960
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540099"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..607)
                     /locus_tag="VN968_16770"
     CDS             complement(146..607)
                     /locus_tag="VN968_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6163 family protein"
                     /protein_id="HXL30920.1"
                     /translation="MSETPTRDQSRDNAMSVAAISSERIETEDSAWTRRLVFFLRIMA
                     VISVAKGLYHWAQVTGFIGGEEDAFENQPMAWQTATVYFAVIELVAAVGLWLATPWGA
                     VVWLTTVVSMAVIELMFPGIYGGSLTVVGVEALMLAAYLALAWMAARERPP"
     gene            869..1930
                     /gene="hemB"
                     /locus_tag="VN968_16775"
     CDS             869..1930
                     /gene="hemB"
                     /locus_tag="VN968_16775"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HXL30921.1"
                     /translation="MAIKFGRPIEMRDAPRRDVLLPGPGLDLVVRPRRNRKAEWARRL
                     VRESVLTTDDLIWPLFVIDGHNKRAGVASMPGVERLSVDQAVRDAERAVKLNIPCIAL
                     FPYTEPGLRDEHGSEAVNPDNLVCKSVRAIKKEFPGLGVLCDVALDPFTSHGHDGLIE
                     DGKILNDETVAVLVRQALVQAEAGCDIIAPSDMMDGRVGAIREGLDAAGFLDVQIMSY
                     AAKYASAFYGPFRDAIGSAKTLTGDKRTYQMDSANSNEALREVELDISEGADMVMVKP
                     GMPYLDIVRRVKDTFAMPTFVYQVSGEYAMISAAAGNGWIDGERAMMESLVAFKRAGA
                     DGILTYFAPQAAERLKAEG"
     gene            2087..2632
                     /locus_tag="VN968_16780"
     CDS             2087..2632
                     /locus_tag="VN968_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HXL30922.1"
                     /translation="MSYSGSGGTGAWRNDGGVPPHAFDPWTQPELFRGVLMRRVFAFL
                     IDLVVLSIPVILAYVFIAVFGLITLGLGWLLFWLVWPAAVIWAIIYYGASLGGPHSAT
                     LGMRLMDLELRTWYGAPGYFVLGAMHAVLFWISVSMLTPLVLLVGLFNGRRRLPHDLI
                     LGTVVVNSSVRTQVAQPARTY"
     gene            2737..3516
                     /locus_tag="VN968_16785"
     CDS             2737..3516
                     /locus_tag="VN968_16785"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HXL30923.1"
                     /translation="MTQHSRDTPQFYLTAPSPCPYLPGRHERKVFTHLVGDKAGDLND
                     LLTHGGFRRSQSIAYRPACDQCRACVSVRVIANEFRPSRNFRKILARNADISGEQRSA
                     VPTSEQYSVFRAYLDERHRHGGMADMTVLDYAMMVEDSHVETRIIEYRRRGGGDAGAT
                     GRDNALVAVALTDVLSDGLSMVYSFFEPSEENRSLGTFMILDHITRARRQGLPYVYLG
                     YWIEGSKKMDYKGRFLPQQRLAPSGWLRIDASGDLPTEPQD"
     gene            complement(3636..5018)
                     /locus_tag="VN968_16790"
     CDS             complement(3636..5018)
                     /locus_tag="VN968_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HXL30924.1"
                     /translation="MDARAPGIPADPLPEAAPSPAPAPGWRRERGEPSLAGMFGSVQT
                     AKQGSFWRKLVAFLGPGYLVAVGYMDPGNWATSLAGGSKFGYALLTVALLSNVMAIIL
                     QSLCTRLGVGAGRDLAQACRDSFPRWVSWPLWLSAEIAITATDLAEVIGTAIGLNLLF
                     HIPLELGVIITAADVFLILALQAFGFRWIEAFVVAMLGVIAACFAIQIAMADPDWGAV
                     LRGFAPTTNILANREMLYLALGILGATVMPHNLYLHSGLVQTRGYGDSIGEKREAITL
                     ATIDSSIALCLALVINASILILAAATFHRAGRTDVAELDQAHAFLAPLLGSTLAPTLF
                     AIALLCCGLNSTITATLSGQIVMEGFLNLRIAPWLRRMVTRMIAIVPAVVVTIWAGEK
                     ATGQLLIFSQVVLSLQLPFAVVPLVMFTASRPKMGPFVAPRWLTIFAALTAAIIIALN
                     AKLVFDFVTG"
     gene            5162..6421
                     /locus_tag="VN968_16795"
     CDS             5162..6421
                     /locus_tag="VN968_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL30925.1"
                     /translation="MSATDHTPRLPPTFNRLAWSNLAAQSADQIALAAAPIVAVLLLG
                     VGEGQTGALQTALTLPFILFAIPTGLLADRISRRWLMAGSEALRAAALAGILVLISLG
                     LLTLPLLALLGFVAVCGTVAYSVAAPALVPSLVSPDALPTANARIELARTVAFASGPA
                     LGGVLVGWVGAAPAFGFAAALSIMAVVLLSGIYEPARAPAPRRHPLQEIGEGAVFVLR
                     HPLLRPVFVTQFIFNTASFLLLAVFVPYAVRHLGLSATGVGATLAMYGVGMVVGALLA
                     TRVMQRLAFGTVIGLGPVTGFVAAAVMAVTTWIPTPWLAAVSFFLLGAGPILWVVSTT
                     TLRQAVTPPRLLGRVSAINIMSYGARPLGSALGAVVGGFYGAELCLYLAAAIFGAQAL
                     VILMSPAVALAQQPEMIGDGPEALRAC"
     gene            6656..7939
                     /locus_tag="VN968_16800"
     CDS             6656..7939
                     /locus_tag="VN968_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HXL30926.1"
                     /translation="MTDRTVDTAPLPEVSSTFAQIAVLAALAATGTLATNILLPSLPQ
                     MAASLKVSTAAVTSAITLFLAVFALGQLVVGPISDRYGRRWPVLTGFAVFCLGSIWCG
                     LATDLPGLLAGRVIQAAGACATSVLSRAIARDLFTGAALGRAMALIMVAMAAAPGFSP
                     LLGGALDHAFGWRSEFVFVAIFAAIGAIAYGAVLGETHHSTRIPLNPLAIAKNYISLI
                     ADRRFLVPAATVSLIMGGLFAMFSAAPRVFIEGLHFTPIQLGLFFAGTVMVVFAAGML
                     ATKLAPRFGLDRAIRGGLVAAATGSIAVLLVSIFHPTFLPFLAAMSVFLLGMGIVNPL
                     GTAQALSPFGEKAGAASALLGFWQMMGAAIGVYLAATVSHEAMLALGIVVTVASLAAV
                     ALYAMRAKSSPLFPRKGFAVVAQGASAAPEVPASS"
     gene            complement(8234..9493)
                     /locus_tag="VN968_16805"
     CDS             complement(8234..9493)
                     /locus_tag="VN968_16805"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL30927.1"
                     /translation="MELAPRLQLINWLVSQGLTGLPENDLIRGFCERCREGGLDLSRG
                     LVFIDTLHPIFEGRGFRWTDTETNEADSFEYGSTSTGDAAQNWRRSTFYHMLENGHDE
                     LRIDLADCATLDFSMIDDLAAKGHRHFVAFVHRFGEAGTIGEMDCVYSYWLTRRADGF
                     SEQGLSALRDLVPVLGLAIKSAAQVEIARTLGRVYLGRDASEQVLRGRISRGVTERIN
                     AVLWFSDLRGSTAISENLAPDEIIPFLNDYAQAAINAIHDEGGDVLKLIGDGVLAMFT
                     RGDMTSAKRAALRAEHRFRRNMAALNVRRAAEDRPITSAYVGLHVGEVFYGNIGSEDR
                     LDFTVVGPAVNEVSRIASMCRSVDRELLASADFCAGLDAAGRRYLVSTGRFALRGIGR
                     AQDLYTLDPVIASDEAVAGRYERYLAG"
     gene            10131..10748
                     /locus_tag="VN968_16810"
     CDS             10131..10748
                     /locus_tag="VN968_16810"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30928.1"
                     /translation="MSWSSVSPDQAEILPGRLFGPELIAKRFGLLKDAISGLSRVAAL
                     WHPTAYGKRTAEGVLRETETAAQSLGLQLQLVPALVPGDLDGAFVAMTRERADAVIQL
                     PSPMLYGEHKRIVELAAKSRLPAMYAAREFVEDGGLMSYGASLPDLFRRAATYVDKIL
                     KGANPADLPVEQPTKLELVFNLKTAKELGLAIPRELLLLADEVIE"
     gene            10981..>11960
                     /locus_tag="VN968_16815"
     CDS             10981..>11960
                     /locus_tag="VN968_16815"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXL30929.1"
                     /translation="MYPRSPTDLTASNLGPALSLDPSSIASVETEVIGQGVGFLCQLA
                     RVKFSYRENARGPKSLVAKYPAAIEQTRGLARQFKFYEREVNFYNLLAKDVSLPSPRC
                     LHASHDLLSDDFLLLLEDLGDRRLGDQLKGCSAEDAFAAIRQLASFHAKWWDSPKLET
                     IDWVPLGESDLNKGGLALYSIAWPLYLQQFGSDLPDDMRRIGERLSDQIVGMLDRVQD
                     RPRTLCHGDYRLDNFFFGERAEHDALTVVDWQIATRSTGTYDVGYFVSQSVPPSLRRE
                     IELDLLRAYHDRLIELGVKNYSFGDCIDDYRWTLMFCLCYPVIGGGLGDPA"
BASE COUNT         2162 a         3754 c         3745 g         2299 t
ORIGIN      
        1 catatggaaa cagcgtcgct cgaattcgaa acgcttgcgt aacgggtatg gcggccgcct
       61 taaggtgacc caaaagttac cgcaaaaatt ttccgaaaat tttatccaac cacgcgaatt
      121 tcgcgggcgg cgcattgcag cggagttatg gcggccgttc gcgcgccgcc atccaagcca
      181 gcgccagata ggccgcgagc atcagcgctt ccacgccgac gacggtgagg ctgccgccat
      241 agattccggg aaacatcagt tcgatcaccg ccatcgatac cacggtggtc agccacacca
      301 ccgcgcccca gggcgtcgcg agccacaatc cgaccgcggc aaccagttcg atcaccgcga
      361 aatagacggt cgcggtctgc caggccatcg gctggttttc gaaggcatct tcctcgccgc
      421 cgatgaagcc ggtgacttgc gcccagtgat agagaccctt ggcgaccgag atcacggcca
      481 tgatgcggag aaaaaatacc agccgccggg tccacgcact gtcttccgtt tcgatccgct
      541 ccgaggagat cgccgccacc gacatggcat tgtcccgcga ctgatcgcgc gttggggttt
      601 cagacatgcg gataattgtt catgatcggg aacgggcctt catcgtagga actcatgatc
      661 gtaggaactc ttgatcgtag gaactcttgg gccctcggcg cggcaaaatc aatccgggcc
      721 ggcgagggtc cggaagctgt ttgagccttg ctgtaggtca aggctacgtg aggtaagcca
      781 tgccagggct gcggtgacgt aggcaataca aggtgttagg attggaaccg gtctaatccg
      841 gtctgccggc tttcggagat acaatcacat ggcgatcaaa ttcgggcgtc cgatcgaaat
      901 gcgcgacgcg ccgcggcggg atgtcctcct ccccggcccc gggctcgatc tggtggttcg
      961 cccgcgccgc aaccggaaag ccgaatgggc gcgccggctg gtgcgggaaa gcgttctcac
     1021 cacggacgac ctgatctggc cgctgttcgt gatcgatggc cacaacaagc gcgcgggcgt
     1081 cgcttcgatg ccgggcgtcg aacggctcag cgtcgatcag gcggtgcgcg atgccgagcg
     1141 ggcggtaaaa ctcaatatcc cctgcatcgc gcttttcccc tacaccgagc ccggcctgcg
     1201 cgacgagcac ggctcggagg cggtcaatcc cgacaatctc gtctgcaaat cggtgcgcgc
     1261 gatcaagaag gaatttccgg gcctcggcgt gctctgcgac gtggcgctcg atcccttcac
     1321 cagccacggc catgacggcc tgatcgagga cggcaagatc ctcaacgatg agacggttgc
     1381 ggtgctggtg cgccaggccc tggtgcaggc cgaagccggc tgcgacatca tcgcgccgtc
     1441 ggacatgatg gacggccggg tcggcgcgat ccgcgaaggc ctcgacgctg ccggattcct
     1501 cgacgtgcag atcatgtcat atgcggcgaa atatgcctcg gcgttttacg gtccgttccg
     1561 cgacgccatc ggctcggcga agacgctgac cggcgacaaa cgcacctatc agatggatag
     1621 cgccaattcc aacgaggcgt tgcgcgaggt cgaactcgat atcagcgagg gcgccgacat
     1681 ggtgatggtg aagccgggga tgccctacct cgacattgtg cggcgggtca aagacacctt
     1741 tgcgatgccg accttcgtct accaggtgtc gggcgaatac gcgatgatat cagccgccgc
     1801 cggcaatgga tggatcgacg gcgaacgggc gatgatggaa agccttgtgg ccttcaagcg
     1861 cgccggcgcc gacggcattc tgacctactt cgcgccgcag gcggcggaaa ggctgaaggc
     1921 ggaggggtga caagctccct ccattcgtcg tcccggcgaa cgccgggacg acgaggtatc
     1981 tgattttcgt cgccggatat tgcggctcgg taacctcgtg gcccttgcaa gcgctttgcc
     2041 agttcccatg tcctgcagcg tgacggcaca gtcgggagga ctgatcatgt cttacagcgg
     2101 ttcgggtggt accggcgcct ggcgcaacga tggcggagtc ccgccgcacg ccttcgatcc
     2161 ctggacgcag cccgaattgt ttcgcggcgt gctcatgcgg cgggtgttcg cattcctgat
     2221 cgacctcgtg gtgctttcga ttccggtgat cctggcatac gtgttcatcg ccgtgttcgg
     2281 gctaatcacg ctgggattgg gttggctgct gttttggctg gtgtggccgg ccgcggtgat
     2341 ctgggccatc atctactatg gtgcatcgct gggcgggccg cattcggcca ccttaggcat
     2401 gcgcttgatg gatctggaac tgcgcacctg gtacggggcg ccgggctatt tcgttctcgg
     2461 cgccatgcat gccgtgctgt tctggatttc ggtgtcgatg ctgacgccgc tggttctttt
     2521 ggtcggcctg ttcaacggcc gccggcggct gccgcacgat ctcattttgg gaaccgtggt
     2581 tgtcaatagt tcggtccgta ctcaggtagc gcaaccggcg cgaacctatt gaaaccggac
     2641 aaaaccattt gccctgattt ggcaaaccga ttgaccgtcg ggctccgcgg cgcgatgctg
     2701 ttcacttgtc gttgccttgg agcttgacgc tatcccgtga cccagcactc gcgtgacacc
     2761 cctcaatttt acctgacggc gccctcgccc tgcccctatc tgccgggccg gcatgaacgc
     2821 aaggtgttca cgcatctggt cggcgacaag gcgggcgatc ttaacgattt gcttacccat
     2881 ggcggcttcc gccgcagcca gtcgatcgcc taccggccgg cctgcgacca atgccgtgcc
     2941 tgcgtttcgg ttcgggtcat cgccaacgag ttccgcccgt cgcgcaattt caggaaaatt
     3001 ctcgcccgca acgccgacat atcaggcgaa cagcgtagcg cggtgccgac ctcggagcag
     3061 tattcggtgt tccgcgccta tctcgacgag cgccaccgcc atggcggcat ggcggacatg
     3121 acggtgctcg attacgcgat gatggtggag gatagccacg tcgaaacccg catcatcgaa
     3181 tatcgccgtc gggggggcgg cgatgccggc gcgaccggac gtgacaacgc gctggtggcg
     3241 gtggcgttga ccgacgtgct cagcgacgga ctgtcgatgg tgtattcgtt cttcgaaccg
     3301 tcggaggaaa atcgctcgct cggcaccttc atgatcctcg accacatcac gcgcgccaga
     3361 cggcagggac tgccttacgt ctatctcggc tactggatcg agggctcgaa gaagatggac
     3421 tacaaaggcc gcttcctgcc gcagcagcgc cttgccccct caggctggct gcgcatcgac
     3481 gcctccggcg acttgccgac cgagccgcag gattgaatca tagcacctca tccggaggag
     3541 ccgcgcgcag cgtggcgtct cgaaggatgg gcggcatcgg ggcctcgtgg ttcgagacgc
     3601 gcgcgatgcg cgctcctcac catgaggatt gaccctcatc ccgtcacgaa atcgaacacc
     3661 agtttcgcat tcagcgcgat gatgatggcc gcggtgagcg cggcgaagat ggtgagccag
     3721 cgcggcgcga cgaacgggcc catcttgggc cggcttgccg tgaacatcac cagcggcacc
     3781 accgcgaacg gcagctgcag gctgagcacc acctggctga agatcagcaa ctgacccgtc
     3841 gctttctccc cggcccagat tgtcacgaca accgccggaa cgatcgcgat catccgcgtc
     3901 accatgcgcc gcagccacgg cgcaatgcgc aggttgagaa agccctccat cacgatctgg
     3961 ccagagagcg tggcggtaat ggtcgagttg agcccgcaac acagaagcgc gatcgcaaac
     4021 aacgtcggcg ccagggtcga gccgagcaat ggcgccagga acgcgtgggc ctggtcgagc
     4081 tcggcgacat cggtcctgcc ggcccggtga aacgtcgccg ccgcgaggat caggatcgag
     4141 gcgttgatga caagcgccag gcacagcgcg atcgaggaat cgatggtcgc cagcgtgatc
     4201 gcctcgcgct tttcgccgat gctgtcgcca tagccgcgcg tctgtaccaa tcccgaatgc
     4261 aaatagagat tatgcggcat cacggtggcg cccaaaatgc cgagcgccag gtagagcatc
     4321 tcgcggttgg ccaaaatgtt cgtggtcggc gcaaagccgc ggagcacggc gccccagtcg
     4381 ggatctgcca tcgcgatctg gatcgcaaag cacgccgcga tcacgcccag catcgcgacg
     4441 acaaaagctt cgatccagcg aaatccgaac gcctgcagcg ccaggatgag aaacacatcg
     4501 gcggcggtga tgatgacccc gagctcgagc ggaatgtgga acaagagatt gaggccgatg
     4561 gcggtgccga tcacttcggc gagatcggtc gcggtgatgg cgatttcggc cgacagccaa
     4621 agcggccacg acacccagcg cggaaaggaa tcgcggcagg cctgcgccag gtcacgcccg
     4681 gcgccgacgc cgagccgcgt gcacaaggac tgcaggatga tcgccatcac gttggagagc
     4741 agcgccaccg tcaatagcgc gtaaccgaat ttggaaccgc cggcgagcga ggtcgcccag
     4801 ttgccgggat ccatataacc gacggctacc agataaccgg ggccgaggaa cgccacgagc
     4861 ttgcgccaga acgacccctg cttggccgtc tgcaccgagc caaacatgcc agccaacgaa
     4921 ggttcgcccc gctccctgcg ccagcccggg gccggtgcag ggctgggggc ggcctcaggc
     4981 agtgggtcag ctggtattcc gggagcacgt gcgtccatcc cagcaagata aggggattcg
     5041 cttttattgc aactcatttg caagagcatc tggactgcgt gttccaggaa ccatgtcggg
     5101 aagcgggtgg gtttgaccgc acccgagccg gacaatgacg gcagaccgaa aggaacaagt
     5161 catgtccgca accgaccaca cgccccgcct gccgccgacc ttcaaccggc tggcatggtc
     5221 caatctcgcc gcccaatcgg ccgaccagat cgcgctcgcc gcagccccga tcgtcgcggt
     5281 gctactgctt ggcgtcggcg aaggccagac cggggcgctg cagaccgcgc tgacgctgcc
     5341 gtttatcctg ttcgcgatcc ccactggcct gctcgcggac cgcatctcgc ggcgctggct
     5401 gatggcgggt tcggaagctc tgcgcgccgc ggcactcgcc ggaatcctcg tgctgatctc
     5461 gctcggcctg ttgacgctgc ccctgctcgc gctgcttggt tttgtcgcgg tctgcggaac
     5521 ggtggcctac agcgtcgcgg cgccggcact ggtgccgtca ctggtatcgc ctgatgcatt
     5581 gcccacggca aatgcgcgga tcgaactggc gcgcacggtc gcgttcgcca gcggcccggc
     5641 gctcggcggc gtgctggtgg gatgggtggg cgccgcgccg gcttttggat tcgccgcggc
     5701 actctccatc atggccgtgg tgttgctgtc cgggatttac gagccggcgc gcgcccccgc
     5761 gccgcgacgc catccgctgc aggagatcgg ggaaggtgct gtgttcgtgt tgcgtcatcc
     5821 gctgctgcgg ccggtgtttg tcacgcaatt catcttcaac accgcttcct tcctgttgct
     5881 cgcggtgttc gtgccctacg cggtgcgtca tctcggattg tcggcgaccg gagtcggcgc
     5941 gacgctcgcg atgtacggcg tcggcatggt ggtcggcgcg ctgctggcga cgcgggtgat
     6001 gcaacggctc gccttcggca ccgtgatcgg acttgggccg gtgaccggct ttgttgccgc
     6061 cgccgtgatg gccgtgacca catggattcc gacgccgtgg cttgccgccg tgagcttttt
     6121 cctgctcggc gcaggtccga tcctgtgggt ggtcagcacc acgacgttgc ggcaagcggt
     6181 gacgccgccg cggctgttgg ggcgggtatc cgccatcaac atcatgagct atggcgcgcg
     6241 cccgctcggc tccgcgctcg gcgccgttgt cggcggtttt tatggcgccg aactatgcct
     6301 ctatctcgcc gccgcgatct tcggcgccca ggcgctggtg atcctgatgt cgcccgcggt
     6361 cgccctggcg cagcagccgg agatgatcgg cgacgggccc gaggcgctgc gggcttgctg
     6421 aggggcgcaa cacgaacgat caacaactcc cttcgtcatg gccgggcctg tcccggccat
     6481 ccacgtcttg cttgctgttt caggaagtaa gacctttcca ttatcatgaa gtaagacgtg
     6541 gatgcccggc acaaggccgg gcatgacgga gtagaatatt ccaaacgtgc ccttggccgt
     6601 ttccagcccc gttttatatg atacatatca tgtcattaat ttacccggag ctgccatgac
     6661 tgatcggacc gtcgacactg ccccgctccc ggaagtctct tcgaccttcg cgcagattgc
     6721 ggtgctcgcg gcgcttgcgg cgaccggaac gcttgccacc aatatcctgt tgccgtcgct
     6781 gccgcagatg gcggcgtcac tgaaagtatc gaccgcggcg gtcacttccg ccatcacgct
     6841 tttcctcgcc gtgttcgccc ttggccagct tgtggtcgga ccgatctcgg atcgctatgg
     6901 ccggcgctgg ccggtgctga cgggctttgc agtgttttgc ctcggcagca tctggtgcgg
     6961 cctcgccacg gacctgccgg gccttctggc cgggcgtgtc attcaggccg cgggcgcctg
     7021 cgccacctcg gtgctgtcgc gcgccattgc ccgcgatctc ttcactggcg cggcgcttgg
     7081 acgcgcgatg gcgctgatca tggtggcgat ggcggcagcg cccggctttt cgccattact
     7141 cggcggcgcg ctcgaccacg ctttcggctg gcgctccgaa ttcgtctttg tcgcaatctt
     7201 tgcggcgatc ggcgcgatcg cctatggcgc ggtgctcggc gaaacccatc attcgacgcg
     7261 gatcccgctc aatccgctcg cgattgcaaa gaactacatc agcctcatcg ccgaccgccg
     7321 ctttctcgtt ccggccgcga ccgtcagcct gatcatgggc ggactgttcg cgatgttttc
     7381 ggcagctccc cgcgttttca tcgagggatt gcacttcacc ccgattcagc tcggcctgtt
     7441 cttcgcgggc actgtcatgg tcgtgttcgc cgccggcatg ctggcaacca aacttgcgcc
     7501 gcgctttggg ctcgatcgcg cgatcagggg cggcctagtg gcggcggcaa ccggcagcat
     7561 cgcggtcctg ctggtatcga tctttcaccc gaccttcctg ccgttcctcg cggccatgtc
     7621 ggtgttcctg ctcggcatgg gcatcgtcaa tccgctgggt accgcgcagg ctctctcccc
     7681 gttcggcgag aaagcaggcg cagcttccgc gctgctcggc ttctggcaga tgatgggggc
     7741 ggcgatcggg gtgtatctcg ccgcgacggt atcgcatgaa gcgatgctcg cgctcggcat
     7801 cgtcgtgacc gtcgcctcgc ttgcggccgt ggcgctctat gccatgcggg caaaatcatc
     7861 gccgttattc ccgcgcaaag gctttgccgt tgtagcgcaa ggtgcgagcg cagcgccgga
     7921 agtaccggcc tcatcctgag gagcttgcaa aacaagcgtc tcgaaggatg acgaaggccc
     7981 tatagcggga ggagtttagg ttgaggcggc agcggcgatc ggtcacttct ccctctcccc
     8041 gctcttcgcg gggtcgagac gagcgaagct cgctcttaga gggttggggt gaggggctgt
     8101 ttccgcaaat tccgagcata ggtgtgcgcg gtgaatcccc ctcacccgga ttgcttcgca
     8161 atccgacctc tccccgcaag cggggagagg tgagtcggac tcgtgcgccg attcaactca
     8221 atgtcatcag gccctagccc gccagataac gctcatagcg cccggcgacg gcctcatccg
     8281 aagcgatgac gggatcgagg gtatagaggt cctgcgcgcg gccgatgccg cgcaacgcaa
     8341 accggccggt ggagaccaga tagcggcgcc cggctgcgtc caggccggcg caaaaatccg
     8401 ccgaagccag gagttcgcga tcgaccgaac ggcacattga tgcaatgcgg ctgacctcgt
     8461 tgaccgccgg tcccacgacg gtgaaatcga gccggtcctc gctgccgatg ttgccataaa
     8521 acacctcgcc gacatgcagc ccgacatagg ccgacgtgat cggtcggtcc tcggcggcgc
     8581 ggcggacatt gagcgcagcc atgttgcgcc tgaagcgatg ctcggcccgc agcgcggcac
     8641 gcttggctga cgtcatgtct cccctggtga acatcgccag cacgccgtcg ccgatcagct
     8701 tcagcacgtc gccgccctca tcgtggatgg cgttgatggc ggcctgggcg tagtcgttga
     8761 gaaacggaat gatctcgtcg ggcgcgaggt tttcgctgat cgcggtcgag ccgcgcaaat
     8821 cggaaaacca cagcacggcg ttgatgcgct cggtgacgcc ccgcgagatg cgcccgcgca
     8881 gcacctgctc ggaagcgtca cgcccgaggt agacccttcc cagcgttctc gcgatctcca
     8941 cttgcgcggc ggatttgatc gcaagcccca gcaccggcac caggtcgcgc agcgccgaca
     9001 ggccctgctc gctaaaacca tccgcacgcc gggtcagcca gtaggaatag acgcagtcca
     9061 tctcgccgat ggttccggct tcgccgaacc gatgcacgaa ggccacgaaa tggcgatgac
     9121 cttttgcggc aagatcgtcg atcatcgaaa aatcgagcgt ggcgcaatca gcgagatcga
     9181 tccgcagttc gtcatgtccg ttttcgagca tgtggtagaa ggtggagcgc cgccagttct
     9241 gcgcggcgtc gcccgtgctg gtcgatccgt attcgaagct atcggcctcg ttggtttccg
     9301 tgtcggtcca gcgaaacccg cggccctcga agatcggatg cagcgtgtcg atgaaaacga
     9361 ggccgcgcga cagatcgaga ccgccctccc ggcagcgctc gcaaaagccg cgaatcagat
     9421 cgttctcggg caggccggtg aggccttggc taaccagcca gttgatcagc tgcagtcgcg
     9481 gcgccagctc cataatccat tatgccgcgt aatcctgtct gacgaaagag ttgcagaata
     9541 aggccgcgcg atcgatcggg cagtgtgatg gccatgacct catcctgaga gcccgaccga
     9601 aaatccatcc ggcatcggac atcgaccgac ggcgcaaaat cgacgcggat gacccaactg
     9661 cgacattggc gcccagcggc agtgctcttg atgccggttt tatccctatc aacgcacctg
     9721 tttgaaccgc taggatgcca tctcctgggc ccgggggctg ataatgaagc ggcgcgagtt
     9781 catcacactg gtcggcggcg cggcagcggc gtggccgctg gccgcgcgtg cgcagccgaa
     9841 gggaaagctc ccgcttatcg gcgtattaaa tcccggctcg accgatacgc ccggggccgt
     9901 tggattttac caagggctgc gggagcttgg ttatgctgag ggttcgaacc tggctatcga
     9961 gcgtcgcttc ggggaatgga atacagagcg attccgggaa gtcgcggacg atctggttcg
    10021 gctcaaggtt gacgtcattg tcgtgatgag cacgacaccg gcccgcgcag ccaagcaagc
    10081 aacaaacata gttcctatcg tcgtggccgg catggccgat ccggtcggcg atgagttggt
    10141 cgtcagtctc gcccgaccag gcggaaatat taccgggacg acttttcggt ccggaattga
    10201 ttgccaaacg cttcggactg ctcaaggacg ccatttccgg cctctcccgc gtcgctgcgc
    10261 tttggcaccc taccgcctac ggcaaacgaa cggcggaagg cgtgttgagg gagaccgaga
    10321 ccgcggccca gtcgctggga ctgcagcttc aattagtgcc ggcccttgtc ccgggcgacc
    10381 tcgatggcgc tttcgtcgca atgacccgag agcgcgccga tgccgtcatt cagttaccga
    10441 gcccgatgct gtatggcgag cacaaacgca ttgtggaatt ggccgcgaag agccggctcc
    10501 cggcgatgta cgctgcaagg gagtttgtgg aagatggggg cctgatgtcc tacggggcaa
    10561 gcttgcccga tctgttccga cgcgccgcca cctatgtgga caaaatcctc aagggcgcca
    10621 acccggccga cctcccggtg gagcagccga caaagctgga gctcgtcttc aatctgaaaa
    10681 cggccaaaga gctggggctc gcaatccctc gcgaactcct gctgttggcc gacgaggtaa
    10741 tcgagtgaac cgcgcgttca tggcaccttt gagacatgcc gagggacgct gagaatgtct
    10801 gcccatcgga gcaggccgga agtggctggc cgaagagaaa aatgacggga tcggcccaac
    10861 tcagacatta gacatagcct gacagtaacc gccatgcttt gatgtgtcga acaaccagcc
    10921 tggcataacc caagaagagg tcgacgatat tttcaaattt ttgattgagg ggggaattac
    10981 atgtatccgc gaagtcccac agatctcact gcttccaatc tgggccccgc gctttctctt
    11041 gatccgtcaa gcatagcctc agtcgaaacc gaggtaatcg gccagggcgt tggatttctc
    11101 tgtcaactgg cgcgagtgaa attctcttac cgggaaaacg cgcgaggtcc aaagtcgctg
    11161 gtcgccaaat atcctgcggc cattgagcaa acccgcggcc tggcacggca gttcaagttc
    11221 tatgaacgtg aagtcaattt ctacaatctc cttgccaaag atgtttcctt gccaagtccg
    11281 cgatgcctgc atgcctctca cgacctgtta agcgatgatt tcctgctgtt gctggaagac
    11341 ctgggcgacc gtcgacttgg cgatcagctg aagggctgct cggctgagga cgccttcgcg
    11401 gcgatccgcc agctcgcgtc atttcacgcc aaatggtggg acagtccgaa acttgagaca
    11461 atcgattggg tgccgctggg agaaagcgac ctgaacaagg gcggacttgc cctttattcc
    11521 atcgcttggc cgctgtatct ccaacagttt ggctccgacc ttccggatga tatgcggcgg
    11581 atcggagagc ggctttccga ccaaatcgtc ggcatgctcg atcgtgtcca ggaccggccg
    11641 cgcacccttt gccacggcga ctatcgactc gacaattttt tcttcgggga gcgagcagaa
    11701 cacgatgccc tcaccgtcgt cgactggcaa attgcaaccc gatctaccgg cacctacgac
    11761 gtcggttact tcgtaagcca gagtgtgccc ccgtcactca ggcgcgagat cgaattggac
    11821 cttctgcgcg cctatcacga tcggctcatc gaacttggcg tcaaaaatta ttctttcggc
    11881 gactgcatcg acgattatcg ttggaccctg atgttttgtc tttgctaccc ggtgattggt
    11941 ggcggccttg gcgaccctgc
//
LOCUS       DATNOI010000420         4086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2431111,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000420 DATNOI010000000
VERSION     DATNOI010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2431111"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VN968_16820"
     CDS             complement(<1..805)
                     /locus_tag="VN968_16820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HXL30930.1"
                     /translation="MSPHHIDSASRRRLLQFLAASPLFARGALAEGLRPSDPVDWAPR
                     DPDKLIADPTQALDVFDFEPVMKKNVPPAHFGYMATGADDEVTLRANREGFRKFELRP
                     RRLVDVSKVDISAEILGAKYDSPIVIAPAGSNRAFHADGEIAVAKAAKAGNHLQILST
                     VATTSIEDAIAARGAPVWFQLYTTQRWEVTEGLVKRAEAAGAPAIAVTLDVRSAAKWE
                     TFARLRRTDARDCGSCHGLTDYLSRKPNFTSIDLGGVSSTVVTNLTWDLI"
     gene            932..2377
                     /locus_tag="VN968_16825"
     CDS             932..2377
                     /locus_tag="VN968_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30931.1"
                     /translation="MTGHVKLATYFCICLVACCTAAHAQQQVIGAPPEASNMKLVGAN
                     DLQARSAYQPTIHHQGDRWIAYIGHHGGTDDIPAPVNPLTGKAEPNGTSIVDVTDPTH
                     PKYLRHIPGQEGKYEAGGAQMVRICDGKALPKGDPNAVYMLRTFGGEAHEIWNVADPA
                     NPVLITRIGGLKDTHKSWWECGTGIAFLVSGAPDWRTRRMTQVYDLGDPAHPVKIRDF
                     GLPGQEPGATGAVPTELHGPISTGPEGNRVYFGYGTNKGGFVQIVDRDKLINGPREPT
                     PDNLRLPEISRLPMSAFNGAHTTFPMRGMPIAEFAHDKDGQNRDIVMIVDEAILNECS
                     EPRQMVWFADVTVENRPMMISSFTVPEASGAFCQRGGRFGSHSSNESMAPVFYKKMAF
                     IAFFNAGVRALDIRDPYHPREVGYFIPSITAATDKRCVPVEGQDRCKVAIQTNNVETD
                     ERGYIYIVDRANTGLHILELTGPARAVAGLP"
     gene            2389..2931
                     /locus_tag="VN968_16830"
     CDS             2389..2931
                     /locus_tag="VN968_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30932.1"
                     /translation="MRSLPVMVVAVLAIGAWSAVAAFEHGRPQVEAWQEIAWPFPRDG
                     WPAGRAFRCSGELCGDDVEVYVRPKIGFCNCDSGVADDDEVDRVADLDLMSHRFTPLE
                     PGKVVRIADMPGRLRSYHLRMSDGSQHTAVGIAVSRRCDLLVAVAQGTGTASGVSRVA
                     LDFLATDALTKWMTSAMDGR"
     gene            3044..3661
                     /locus_tag="VN968_16835"
     CDS             3044..3661
                     /locus_tag="VN968_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HXL30933.1"
                     /translation="MSLQVYLAFLAACIALALLPGPVVTLVIANGLRHGTRAALTNIL
                     GVQTGLAIVIGIVAVGLTSLMATMGYWFDWVRFAGAAYLVWLGIKLIRAPVEGVSAEA
                     PPPPPRGGFFLQGFVVLLSNPKVLVFFGAFIPQFMDMSRDHVSQVALLGVTFMITGAI
                     TDSIYAILAGRARKFFSARRTRLLSRVSGGFMIGGGVWLALTRAR"
     gene            complement(3674..>4086)
                     /locus_tag="VN968_16840"
     CDS             complement(3674..>4086)
                     /locus_tag="VN968_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HXL30934.1"
                     /translation="FDPYHRRIAEVIDARLREGVPTVLVSLHSFTPVYAGIARPWHIG
                     TLYQRDTRLPRLLLKQLRGEGDLVVGDNEPYAVSDETDYTIPVHGEARGLMNTGIEIR
                     QDLIADPAGQHQWADRLARIFGEIETALRGQRLV"
BASE COUNT          707 a         1276 c         1308 g          795 t
ORIGIN      
        1 tgatcaaatc ccaggtcagg ttggtgacga ccgtgctgct cacgccgccg agatcgatgc
       61 tggtaaaatt cggcttccgc gacagatagt cggtgaggcc atgacagctg ccgcagtcgc
      121 gggcgtcggt gcgccgcaat ctggcaaacg tctcccattt cgctgccgat ctcacgtcga
      181 gtgtcaccgc gattgccggc gcgcccgcgg cctccgcccg cttgaccagg ccttcggtca
      241 cttcccagcg ctgggtcgta tagagctgaa accacaccgg cgcgccgcgc gcggcgatcg
      301 catcctcgat cgatgttgtg gcgacggtgg acagaatttg cagatggttg ccggctttcg
      361 ccgccttggc gacggcgatt tcgccatcgg cgtgaaaagc gcggttgctg cccgccggcg
      421 caatgacgat cgggctgtcg tatttcgcgc cgaggatttc ggcactgatg tcgactttac
      481 tgacatcgac gagtcgccgc ggccgcagct cgaatttccg gaaaccctcg cggttggcgc
      541 gcaaggtcac ttcgtcatcg gccccggtgg ccatgtaacc gaaatgcgcc ggcggaacat
      601 tctttttcat gaccggctcg aaatcgaaaa cgtcgagcgc ctgtgtgggg tcggcaatca
      661 gtttgtcggg gtcgcgcggc gcccagtcaa ccggatccga tggccgcagc ccttccgcca
      721 aggcgccgcg cgcgaacaac gggctcgcgg ccaaaaattg cagcaaacgg cgacggctgg
      781 cggagtcaat atgatgcggc gacatggttg cctccccaaa agaagagatg ttcgcgatcg
      841 accaagcgta gtgcaatccg gggacgcttc gctatgatat ctttctcaat cagccagcga
      901 agactggctt cgataacctg ggtggaaaaa catgaccggt cacgtcaaac tggcaacgta
      961 tttttgcata tgtcttgtcg cttgctgcac cgccgcgcat gcgcagcagc aggtgattgg
     1021 cgctccgccg gaagcgtcca acatgaagct ggtcggcgct aacgacctgc aggcgcgcag
     1081 cgcctaccag ccgaccatcc atcatcaggg cgatcgctgg attgcctata tcggccatca
     1141 cggtggaacc gacgatatcc cggcgccggt caatccgttg accggcaagg ccgagccgaa
     1201 cggaacgtcg atcgtggatg tcaccgatcc cacacatcca aagtatctgc gccacattcc
     1261 cggccaggaa ggaaaatacg aggccggcgg cgcgcagatg gtgcgcatct gcgacggcaa
     1321 ggcgctgccc aagggcgatc ccaacgcggt ctacatgctg cgcacgtttg gcggcgaagc
     1381 gcatgaaatc tggaatgtgg cggatcccgc caatccggtt ctcatcacgc ggatcggcgg
     1441 cttgaaggat acccacaaga gctggtggga atgcgggacc ggcattgcct ttctggtgtc
     1501 gggcgcaccc gactggcgca cgcgccgcat gactcaagtg tacgacctcg gcgatcccgc
     1561 gcacccggtc aagattcgcg atttcggcct tcccggtcag gagcccggcg caaccggcgc
     1621 ggtgccgact gagttacacg ggccgatctc gaccggcccc gaaggcaacc gcgtctattt
     1681 cggctacggc accaacaagg gcggcttcgt gcagatcgtc gatcgcgaca agctgatcaa
     1741 cggaccgagg gagccgacgc cggataatct tcgtctgccg gaaatctcaa ggctgccgat
     1801 gtccgcgttc aacggcgccc acaccacctt tccgatgcgt ggaatgccga tcgccgaatt
     1861 cgcccacgac aaggacggcc agaaccgcga catcgtgatg atcgtcgacg aggcgatttt
     1921 gaacgaatgc agcgagccgc ggcagatggt gtggttcgcc gacgtcacgg tcgagaaccg
     1981 cccgatgatg atttcgagtt tcaccgtgcc ggaggcgagc ggtgcgttct gccaacgggg
     2041 agggcggttc ggctcgcatt cctccaatga gagcatggcg ccggtgtttt acaagaagat
     2101 ggcgttcatc gccttcttca atgccggcgt gcgggcgctc gacattcgcg atccctatca
     2161 tcccagggaa gtcggctatt tcatcccctc gatcacggcg gccaccgaca agcgttgcgt
     2221 cccggttgaa ggccaggatc gctgcaaggt cgcgatccag accaacaatg tcgaaaccga
     2281 tgaacgcggt tatatctaca tcgtcgatcg cgccaacacc ggcctgcata ttctggaact
     2341 gaccggcccc gcgcgcgccg tcgccggcct gccctgatgg tgtgaacgat gcggagcctg
     2401 ccggtcatgg tggttgcggt gttggcgatc ggcgcatggt ccgccgttgc agcctttgag
     2461 catggccggc cgcaggtcga ggcctggcag gagatcgcct ggccgtttcc gcgcgacggc
     2521 tggcccgcgg gccgcgcgtt ccgttgctcc ggtgagttgt gtggtgacga tgtcgaagtg
     2581 tatgtgcggc cgaagatcgg tttctgcaat tgcgacagcg gcgtcgccga tgacgacgaa
     2641 gtcgaccgcg tcgccgatct cgatctgatg agccaccggt tcacgccgct cgagccgggc
     2701 aaggtggtgc ggattgccga tatgcccggt cgcctgcgca gctatcatct caggatgtcc
     2761 gacggctcac agcatacggc ggtcggcatc gcggtgtcgc ggcgctgcga tctcctggtc
     2821 gcggtcgcgc agggcacggg cacggcgtcg ggcgtttcgc gcgtggcgct ggattttctt
     2881 gcgacggacg ccctaacaaa atggatgacg tcggcgatgg atgggcgttg aggcttcgct
     2941 ctcatccctt ctccccttgt gcgagagggg aagaggcggc ttctcccgtg cgatatgatc
     3001 gggcataatc gaattgccgt ttcaaacaac cgcgagtccc ctcatgtccc tccaggtcta
     3061 tctcgcattt ctcgccgcct gcatcgcgct ggcgctgctg ccgggtccgg tggtgacgct
     3121 ggtgatcgcc aatggcctgc gccatggcac ccgcgcggcg ctgaccaaca tcttgggcgt
     3181 ccagacgggc ctcgcgatcg tgatcggcat tgtcgccgtc gggctgacct cgctgatggc
     3241 gaccatgggc tactggttcg attgggtgcg gtttgccggc gccgcctatc tggtctggct
     3301 cggcatcaag ctcattcgtg ctccggttga aggcgttagc gccgaggcgc cgccgccccc
     3361 gccgcgcggc gggtttttcc tgcagggttt tgtggtgctg ctctcgaacc ccaaagtgct
     3421 ggtgttcttc ggcgcgttca ttccgcaatt catggacatg agcagggatc atgtctcgca
     3481 agtggcgctg ctcggcgtca ccttcatgat caccggcgcg atcactgatt cgatctacgc
     3541 catactggct ggccgcgcgc gaaaattctt ctcggcgcgg cggacacggc tgctgtcgcg
     3601 cgtttccggc ggcttcatga tcggcggcgg cgtctggctg gcactgacgc gggcgcggtg
     3661 acgcggttcg cagctatacc agccgctgcc cgcgcaacgc tgtttcgatc tcgccaaaaa
     3721 ttcgtgccag ccgatccgcc cattggtgct ggccggccgg atcggcgatc aggtcctggc
     3781 gaatttcgat tccggtgttc atcaggccgc gcgcctcgcc gtgcacgggg atggtgtagt
     3841 cggtctcgtc gctcaccgca tagggctcgt tgtcgccgac caccagatcg ccttcgccgc
     3901 gtagctgctt gagcaggagc cgtggcagcc ttgtatcgcg ttgatagagc gtgccgatat
     3961 gccacggccg cgcgatgccg gcgtagaccg gcgtaaaact atgcagcgac accagcaccg
     4021 tcggaacgcc ttcgcgcagg cgcgcgtcga tgacctcggc gatgcggcga tggtagggat
     4081 cgaaga
//
LOCUS       DATNOI010000421         3645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1797233,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000421 DATNOI010000000
VERSION     DATNOI010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797233"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VN968_16845"
     CDS             complement(<1..178)
                     /locus_tag="VN968_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HXL30935.1"
                     /translation="MSARRIFTRPARLAAILPYLWMVLFFLVPFGFVLKISLSQTAIA
                     QPPYLPVFDPTQGFA"
     gene            complement(175..1323)
                     /locus_tag="VN968_16850"
     CDS             complement(175..1323)
                     /locus_tag="VN968_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL30936.1"
                     /translation="MAEEFSKPEAGADAAEIPLLRINAVVKKFGGFRAVDRVSLDIRA
                     GEFFALLGPSGCGKTTLLRMLAGFETPDAGWILLDGKDIAQVLPHQRPINMMFQNYAL
                     FPHLTVRDNIAFGLKRAGMPRPQIDTRVAEMVALVRLEGLEQRKPDQLSGGQKQRVAL
                     ARSLARRPKVLLLDEPLAALDKKLREKTQLELMELQRRLGMTFIIVTHDQEEAMTVAS
                     RIGVMDAGRLEQVATPRELYEAPTSRWIAEFVGDVNMFEGQIESREAGRLAVSTRAAG
                     TIMVAEPRQPITKTIVAVAIRPEKVKLSRRGPASDEGNAHSINRLEGIVTDVSYLGGI
                     TTYKVKLDSGAVVRSSMANTARIDLDAYNASQRVVAWFTPDDCVVLEQ"
     gene            1572..2447
                     /locus_tag="VN968_16855"
     CDS             1572..2447
                     /locus_tag="VN968_16855"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL30937.1"
                     /translation="MVVPFPAGGPTDIVARPLAQMLGEALKAIVLVDNRGGAGGSLGA
                     DVVAKSPPDGQTLLMATVGTHAINPALYRNLSYDAESDFTPVALVALAPVAVVVHPSQ
                     PMTNLAELVDLAKHMPGKLNYGSAGVGTPGHLTAEMFKAAAAIDIQHIPYKGSAPAVT
                     DLLGGQIQIMFDPLQSVLSNVLAGKLRALAISSKARSPVLLDVPTIAGSGYNGFETTA
                     WWGVFAPARMPDGLTATIAGEVDRIVNSEAFRRQLEPLRVIPTVLSRGAFSEFQHSEL
                     AKWAKAVHDGGVRID"
     gene            2453..>3645
                     /locus_tag="VN968_16860"
     CDS             2453..>3645
                     /locus_tag="VN968_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HXL30938.1"
                     /translation="MKLNDEESDILAGKAGPVPQPALRHQIKVGEFFGAEDMVPVTQA
                     HLMADTESLGEAGVRWLEGLAATTGGQRRVRIPTITDPRGTDFAKADLLGQTSAMLGL
                     ERRAISAFEALGVAMTNTCINYQTIQAPTRNEHVAFGDTGVVIYSNSICGARSNFEGG
                     PSALAAGLTGRTPRYGFHLPEMRRATLRMQVEFTPTSLNEWGALGGVIGRIAGNYWAV
                     PVIEGIEGAPMSDQLKHFGASMASFGSIALFHLVGLTPEAGKLTDVAAPGLPSHRVTM
                     ADVDQLQRSCHTDDGVDVVVFSAPQLSLFELSDLAELCEGRRFKVPLLAVTSPQVKPD
                     ADRMGYTARIEAAGGSVLSGMCFYQSYAREISEARGWKRLATNSAKLVNILGGYGYRP
                     MLASM"
BASE COUNT          664 a         1154 c         1152 g          675 t
ORIGIN      
        1 ccgcaaaacc ctgggtcgga tcgaataccg gcagataggg cggctgcgcg atcgcggttt
       61 gcgacaggct gatcttgagc acgaagccga acggcaccag gaagaacagc accatccaca
      121 gatagggcag gatcgcggcg agccgcgccg ggcgggtgaa gatgcggcgg gcgctcattg
      181 ctccagcacc acgcagtcgt cgggggtgaa ccacgccacc acgcgctggc ttgcgttgta
      241 ggcatcgaga tcgatccgcg cggtgttggc catcgacgaa cgcaccaccg cgccgctgtc
      301 gagcttcacc ttgtaggtcg tgatgccgcc gagataactg acgtcggtga cgatgccttc
      361 cagccggttg atggaatgcg cattgccctc gtccgacgct gggccgcggc gcgacagctt
      421 gaccttttcg ggccggatcg cgacggcgac gatggttttc gtgatcggct ggcgcggctc
      481 cgcgaccatg atcgttccgg cggcccgtgt cgaaaccgcc agccgacccg cctcgcgcga
      541 ctcgatctgt ccctcgaaca tattgacgtc gccgacgaac tcggcgatcc agcgcgaagt
      601 gggcgcctcg taaagctcgc gcggggttgc gacctgttcg agacggccgg catccatcac
      661 gccgatccgg ctcgccaccg tcatcgcctc ctcctgatcg tgggtgacga tgatgaaggt
      721 catgccgagc cgccgctgca gctccatcag ttcgagctgg gttttttcgc gcagcttctt
      781 gtcgagcgcg gccagcggct cgtcgagcag caacactttc gggcggcgcg ccaaggagcg
      841 ggccagcgcc acccgctgtt tttgaccgcc ggagagctga tcgggcttgc gttgctccag
      901 gccctcgagc ctgaccagcg cgaccatctc cgccacgcgg gtgtcgattt ggggacgcgg
      961 catgccggcg cgcttcaaac cgaaagcgat gttgtcccgc acggtgagat ggggaaacaa
     1021 cgcataattc tggaacatca tgttgatcgg gcgctggtgc ggcagaacct gcgcgatgtc
     1081 cttgccatca agcagaatcc agcctgcgtc cggcgtctca aaaccggcga gcatccgtag
     1141 tagcgttgtc ttgccgcaac cgctgggacc aagcagcgca aaaaactcgc cggccctgat
     1201 gtcgagcgat acccgatcca cagcgcggaa gccgccgaat ttttttacca ccgcattgat
     1261 gcgcagcagg ggaatctcgg ccgcgtctgc gcccgcttcg ggtttcgaaa attcctcagc
     1321 catattcctc gccagcccaa tctccgatct acgctaatga ctgatcgaag cgggctcaac
     1381 ctcttccggg ctcccatcga gcgagaacca tcgttaccat tgactcccac tcaaaagaat
     1441 tggcagcttg aacggcgagg gacgctgatg cagcgcagac aattcctgag aattgcttcg
     1501 gctgcggctg gtgccggcct gctgacgcga accgggcccg cgtggacgca atccctcgcc
     1561 ggcaaatccg catggttgtg ccgtttccgg ctggcgggcc aaccgacatc gtggcccgcc
     1621 ccctcgctca aatgttgggg gaagcgctta aagcgattgt cctggtcgac aatcgcgggg
     1681 gcgcaggtgg ttcgttggga gccgacgtcg tcgccaagtc gccccccgat ggccaaacgc
     1741 tcttgatggc gacggtcggc acgcacgcca tcaaccccgc tttgtacagg aacttgtcct
     1801 acgatgctga aagcgatttc acgcctgtcg cgctggtggc gctggctccg gtggccgtcg
     1861 tcgtgcaccc ctcgcagccg atgacaaatc tcgccgagct ggttgatctg gccaagcaca
     1921 tgcctggcaa gctgaactac ggctcggccg gcgtcggcac gcccgggcac ctgaccgccg
     1981 agatgttcaa ggccgccgca gccatcgata ttcagcacat tccctacaag ggcagcgcgc
     2041 ccgccgtgac cgacctcttg ggcggccaga tacaaatcat gttcgatccg ctgcaatcgg
     2101 tgctgtcgaa cgtgttggcg ggcaagctgc gcgcccttgc gatctcaagc aaggcgcgct
     2161 cacccgtgct gctcgatgtc cctaccatcg ccgggtccgg atataatggt tttgaaacga
     2221 ccgcgtggtg gggcgtgttt gcgccggcca gaatgccgga cgggctgacc gccacaatcg
     2281 ccggcgaagt tgatcggatc gtcaacagcg aggcgttccg ccgccagttg gagcctctgc
     2341 gcgtgatccc gacagtactc agtcgcggtg ctttttccga atttcagcat agcgaactgg
     2401 cgaaatgggc caaggcggtg catgacggtg gcgtaaggat tgattgacct cgatgaaact
     2461 caacgacgaa gagagcgata tcctggccgg caaggctggc ccggttccgc agccggcctt
     2521 gcggcaccag atcaaggtcg gcgaattttt cggcgccgaa gatatggtgc cggtgacgca
     2581 agcccacctg atggccgata ccgaaagcct tggcgaagcc ggcgtgcggt ggctggaggg
     2641 cctcgctgcg accacgggtg ggcagcggcg cgtccggatt ccaaccatca ccgatccgcg
     2701 cggcaccgac ttcgccaaag ccgatttgct gggtcagacg tcagcgatgc tcggtctcga
     2761 gaggcgggcg atttccgcat tcgaagcgct cggtgtcgcg atgaccaaca cctgcatcaa
     2821 ctatcagacc attcaggctc cgacgcgcaa cgagcacgtc gccttcggcg acaccggcgt
     2881 ggtcatctat tcaaactcaa tatgcggcgc acggtcgaat tttgagggcg gcccctcggc
     2941 gctggccgcc ggccttaccg gccgaacgcc ccgctacggc tttcaccttc cggaaatgcg
     3001 ccgggccact ctgcgcatgc aggtggagtt cacgccgaca tccctcaacg agtggggcgc
     3061 gcttggcggg gtgattgggc gtatcgccgg taactactgg gcggtgcctg tcatcgaggg
     3121 gatcgaggga gcgccgatgt cggaccagct caagcatttc ggcgcctcga tggcgagctt
     3181 cggctcgatt gccttgtttc atctggtggg gctgacgccc gaagccggga aactgactga
     3241 cgtggccgcg ccgggtttgc caagccatcg cgtgaccatg gccgacgtcg accaattgca
     3301 aagatcctgt cataccgatg acggggtgga cgtcgtggtc ttctcggcgc cgcagctcag
     3361 cctttttgaa ttgtccgatt tggccgaact gtgcgaaggc cggcgcttca aggtgccgct
     3421 gctcgcggtc accagccccc aggtaaaacc ggacgctgac cgcatgggct acacggcgcg
     3481 aatagaagct gccggcggca gcgtcctatc cggcatgtgc ttctatcaat cctatgcgcg
     3541 agagatttcc gaggccaggg gctggaagcg cctggccacc aacagtgcaa agctggtcaa
     3601 tattctgggc gggtacggtt accggccgat gctggcgtcg atgga
//
LOCUS       DATNOI010000422        30858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_550179,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000422 DATNOI010000000
VERSION     DATNOI010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 30858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30858
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550179"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VN968_16865"
     CDS             complement(<1..183)
                     /locus_tag="VN968_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HXL30939.1"
                     /translation="MTDRFDYVIVGAGSSGCVLANRLSEDAITSICVLEAGPRDWHPY
                     IHLPAGFIKTFHMKGIN"
     gene            complement(204..797)
                     /locus_tag="VN968_16870"
     CDS             complement(204..797)
                     /locus_tag="VN968_16870"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HXL30940.1"
                     /translation="MPWLEPISLSGPHAQLKPLSQDHCDGLTEAVKDGELWKLWYTFI
                     PRAEDMRKEIDRRLGLQAAGSMLPFTVFDADGNIAGMTTYMNVDAPNRRVEIGSTWYA
                     KRVQRSALNTQCKLLLLSHAFEKLDCIAVEFRTHFFNHQSRRGIERLGAKLDGILRNH
                     QIAPNGTLRDTVVYSIIAGEWPTVKAHLNYQLNEKPR"
     gene            complement(913..1842)
                     /locus_tag="VN968_16875"
     CDS             complement(913..1842)
                     /locus_tag="VN968_16875"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXL30941.1"
                     /translation="MPDVRVLATDLEFPEGPVVLPDGSVVLVEIRGQRLTRVWPDGRK
                     QVVAEIPGGPNGAAMGPDGKIYICNNGGFSWIPSRGTMMPGAPEPHEYIGGSIQRVDL
                     ATGEVETLFDKCGKHSLKGPNDLVFDKHGGLWFTDLGKRRARDMDVGAFYYMKPGATE
                     VVEAVFGMMPTNGIGLSPDENTVYVAETPTARLWAFDLSAPGEIKPRDVIYRGERGKP
                     MVGLGGYQMFDSLAVEASGNVCVATLISGCISVIAPDGAIVEQVPTGDRVTTNIAFGG
                     PELKTAYITLSGKGQLVAMDWARPGLPLNFLNK"
     gene            complement(2059..2892)
                     /locus_tag="VN968_16880"
     CDS             complement(2059..2892)
                     /locus_tag="VN968_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXL30942.1"
                     /translation="MNPAQKALWFIESHLADALTLDEIAAIGGVSRFHMVRAFAAATG
                     LSVMRYVRARRLTEAARALANGAPDILTLALDADYGSHEAFTRAFRGHFGVTPEAVRA
                     STCLDGIKLQEPIQMDSTLTGKLHAPRFETSRPLLVAGISERYNHENGAGIPNQWQRF
                     HQAVENIPGRIGKVAYGVCCNGDDAGNFDYIAGVEVSDFSDLPREFHSVRIPEQRYAV
                     FTHSDHISTIRRTVNTIWNHWLPASGLKAADAPNFERYDENFDPLTGNGGLEIWVPVR
                     E"
     gene            3154..4383
                     /locus_tag="VN968_16885"
     CDS             3154..4383
                     /locus_tag="VN968_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL30943.1"
                     /translation="MHYRVLSHARSVGLAGLLALAVASAANAQKKYDPGATDTEIKIG
                     NIMPYSGPASAYATIGKTEAAYFNKINADGGINGRKINFISYDDGYSPPKAVEQARKL
                     VEADEVLLIFNPLGTPSNSAIQKYMNAKKVPQIFVSSGAAKWNDPKNFPWTIGWQPSY
                     QVEARIYAAYILKNHPGKTIGVLYQNDDFGKDYVIGLREGLGDQGNKLIVVESSYETT
                     SPTVDSQVVQIKGANPDIFVNVSTPKFAAQAIKKIGELAWHPVQFLTNVSASVGGVMK
                     PAGYENDQDILSAACLKDPQDPKWKDDPAMNEWRAFMTKWYPEGDQADAATTFGYGVA
                     KGLEQVLRQCGDDLTRENIMRQAANLNFEIGVYLPGTHIKTSPTDFAPLEQLQMMRFK
                     GESWDLFGPIMSGEKNS"
     gene            complement(4559..4942)
                     /locus_tag="VN968_16890"
     CDS             complement(4559..4942)
                     /locus_tag="VN968_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30944.1"
                     /translation="MIGHIALVASVVLTSCVLVPVDARAQDVPGIEICTVEKTMERRT
                     SCLQSNVDFLQKTITKLAADHQQKLDAATRQIDGLKATVASLQKIVDDLQAAQKKALE
                     DQKKADAPPAKDNARMPVDAKDGAK"
     gene            5292..5513
                     /locus_tag="VN968_16895"
     CDS             5292..5513
                     /locus_tag="VN968_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30945.1"
                     /translation="MATRERRLAEFNGNGEPPPDQPVQVLCEDHSGTYQLPFACRFVG
                     GEWRNNESGGTVEATVVGWRLPRTASRAL"
     gene            complement(5738..6172)
                     /locus_tag="VN968_16900"
     CDS             complement(5738..6172)
                     /locus_tag="VN968_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL30946.1"
                     /translation="MNQHAVVNTFSHVKPGDTEFTGGGLRDFFLYRDLGIAAATGGQV
                     ICHLVKANPEMPPEDGTGWHKHECDFQIVIMTKGWARFMYEDKSTLVQAGDVVHQRPG
                     IVHYLYDYSPDMEYLEIVSPADFKTVDVPPATDKVPPVTPWK"
     gene            complement(6508..7164)
                     /locus_tag="VN968_16905"
     CDS             complement(6508..7164)
                     /locus_tag="VN968_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXL30947.1"
                     /translation="MAKATLTISSKNYSSWSLRGWLLTKFSGLEFEEIITAPDDASAR
                     AEILLLSSSILVPCLRHDGATVWDTLAIAEYLNEVMPDAGLLPADRVLRAHCRSICGE
                     IHSGFTTLRASLPVNLKGHFPGFKIWSRAQADIDRVCAIWRECLAKSGGPFLFGERRT
                     MADAMYAPVVTRFMTYDVKLEPRLKAYADMVMALPEMQEWIEAAKVEPADIEELEVEY
                     "
     gene            7327..8547
                     /gene="phaZ"
                     /locus_tag="VN968_16910"
     CDS             7327..8547
                     /gene="phaZ"
                     /locus_tag="VN968_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HXL30948.1"
                     /translation="MMMMYQAYQNHMDLTAPWRTGAASALKYLNLVPQGVSDKLFGRL
                     AAALELISRTSLTYTRPPYGIDKVLVGNRELEVTEEVAYATPFGSLLHFKKQNSPEQP
                     RMLLVAPMSGHFATLLRGTVKTLLQDHDVYITDWHNPRDIPLDQGRFGLDEYTEHLIT
                     FLGQLGPRAHMVAICQPSVSALAAAAIMSEDNHPARPATLTLMAGPIDTRIQPTKVND
                     FAKSKPLKWFEENLINYVPFQCKGAFRQVYPGFIQLTAFVSMNLERHIKSHVDLADHL
                     AKGEKEKAETIKTFYDEYFAVMDLSADFYIETIRDVFHEHLLPLGKLTYRGRPVSPAA
                     IKRMGLMTVEGEKDDICSIGQTLAAQDLCTGVRAYRKVHHMQAGVGHYGVFSGRRWNN
                     EIYPLLRDFIHVNS"
     gene            8759..9556
                     /locus_tag="VN968_16915"
     CDS             8759..9556
                     /locus_tag="VN968_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXL30949.1"
                     /translation="MKSISANIRSLVIGAIGALLLAGSANAAEVRVMISGGLTAAYKE
                     LVPEFERLTGNKVLTAYGPSMGTTVNAIPVRLERGEPADVLIMVGYALGDLVKQGKVI
                     ADSRVDLVKSPIGIAVKSGAPKPDISSADAVKRALLAAKSIAYSDSASGVYVSTEMFE
                     KLGVAEAMKDKARKIPATPVGEIVAHGDAEIGLQQISELKPVEGIDIVGPLPDDLQKI
                     TVFSAGIATVSREPDAGKALIKFLASPAASAVIVRSGLEPIPVGATN"
     gene            complement(9576..10319)
                     /locus_tag="VN968_16920"
     CDS             complement(9576..10319)
                     /locus_tag="VN968_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXL30950.1"
                     /translation="MAVPASADALKDEIAPTGKLRVAIAISTAGGAFWSTKNEAGGYA
                     GVPVDLGKEMAAQLAVPVEYVAYDNSGQITDAAAKGIWDVTFLPQDAVRMTKMGFGPI
                     YQVTDATYIVKAGSSVTNFQTLDQPGIKVAAEYNTTTMRGAVAHLKNAKVIEYQTYDE
                     IFALLKNGEIDAFALSRDQLNDMAKKIPGTRVLDETFKQTVTAVAVPLDHPLALAFVN
                     KFMTDAISNGMLRRAYDNNGLRDAPIRVK"
     gene            complement(10587..11762)
                     /locus_tag="VN968_16925"
     CDS             complement(10587..11762)
                     /locus_tag="VN968_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL30951.1"
                     /translation="MNSPNRVIGFVNAAHFIDHYSMLIFAAAVIVMGPALGMAYSELL
                     PYATPGFVAFGAGSLLTGWLGDRWSRRHMMVIFFAGIGASMIAVGLVQTPLQLGAALL
                     AIGLFASIYHPVGTAMIVSYADRLGREMGLNGVWGNLGVASSALVTGVVGQCLGWRWA
                     FIIPGIVTILIGLAFARTVVHEDRSGFKQAAAQARVAKNEMWRVFVALLIVVIAVSTT
                     FNAVTVALPKLFAERLSDLTKSPALLGVIAACVYVFGAMTQYTIGKLIDRYSLKTVML
                     PLSFMLAPFLYLAGTLSNLPLILASIGIVMGAFGQVTVNDAMVGKYTSEEWRSRAYAV
                     RYFIGFTAAGVSVGLVAWLYEQGGFVIMLHAFGALCLLVIAAAIILPQEIKVPAVAN"
     gene            11825..12625
                     /locus_tag="VN968_16930"
     CDS             11825..12625
                     /locus_tag="VN968_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL30952.1"
                     /translation="MNIAETPIAAIRGSHRATGDGVHLVARHYRKGVRLDTHMHREAQ
                     LVYAAKGTMQVTTPKGRWLVPPDRAVWVPARLEHSIDVLADIEMRTLYFDLAWLARED
                     RSESLDCEFVVRVSRLLHETILALFDGQGNPDRTGLLVKLAVLELHHAEDSATFIPLP
                     QEPRCRRAADIVLGDPTAAHEIETLAREVGTSARTLSRLFSSETQLSFKSWCQRARIA
                     AAIERLSTQADVSVKQLASELGYASVPAFSHAFRQVTGKTPTQFAETK"
     gene            12860..14215
                     /locus_tag="VN968_16935"
     CDS             12860..14215
                     /locus_tag="VN968_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HXL30953.1"
                     /translation="MSSNAFFSDLLASISERGRTLLRRAGSSDTKRHASDLIELCEAL
                     LSGRGEASGTAMAREVLDHYHSLDEAGRLAFFETLTRDFGPDREKLAEAIEAWRAQPT
                     DADASDLHFASEPRRQELIRQLNRAPGGTSALVAMRADLLGAMNGHKDLAALDRDVVH
                     LLSSWFNRGFLVLRRIDWSTPANILEQIIRYEAVHEIRDWDDLRRRIDPIDRRCYAFF
                     HPALVDEPLIFVEVALTESIPAAIAPLLAEDRQPVPVERARTAVFYSISNTQRGLGGI
                     SFGNFLIKQVVEELRRELPKLENFVTLSPVPGFMKWLKQASDVPVSDEERSQLAHLDQ
                     PDWFEHAELTSQLRSLIEPLAAHYFLKARTSKGRLIDSVARFHLGNGARLERIDWLGD
                     LSPKGMRESAGIMVNYLYRLEDIEKNHEAYANQGEIAASSAVKKLLKTEGRRLLDMRL
                     S"
     gene            complement(14328..15497)
                     /locus_tag="VN968_16940"
     CDS             complement(14328..15497)
                     /locus_tag="VN968_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXL30954.1"
                     /translation="MSVRIVDVREITKPISSPIRNAYIDFSKMTTSLVAVVTDVMHDG
                     KPVVGYGFNSNGRYGQGGLIRERFAPRLMEADPKTLLDETSDNLDPDKVWATLMSNEK
                     PGGHGERSVAVGTIDMAVWDAVAKIARKPLFRLLAERHGRNADPRVFVYAAGGYYYPG
                     KGLSMLRGEMRGYLDRGYNVVKMKIGGAPIAEDRMRIEAVLEEIGSHAQLAVDANGRF
                     DLETAIAYAKMLRDYTLFWYEEAGDPLDFQLQAALAEFYPGPMATGENLFSHQDARNL
                     IRYGGMRPDRDWLQFDCALSYGLCEYQRTLAVLQTQGWSASRCIPHGGHQMSLNIAAG
                     LGLGGNESYPDLFQPYGGFPDGVRVENGHITMPDLPGIGFEGKSDLYAEMKALAS"
     gene            complement(15642..15902)
                     /locus_tag="VN968_16945"
     CDS             complement(15642..15902)
                     /locus_tag="VN968_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HXL30955.1"
                     /translation="MTGQLTDQLPDRLSSDPKSPYYNADVLARDVGIRFKGVEKTNVE
                     EYCVSEGWVRVTAGNAKDRYGNPLTIKVHGPVEPYFRDKASS"
     gene            complement(15920..16396)
                     /locus_tag="VN968_16950"
     CDS             complement(15920..16396)
                     /locus_tag="VN968_16950"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HXL30956.1"
                     /translation="MPGVHDFTAKSLAGEDVPLKRFEGQVLLIVNTASACGFTPQYKG
                     LETLHQALNPRGFSVLGFPCNQFGSQEPGDSRQIEQFCASNYAITFPMFAKIDVNGTG
                     AHPLFNYLKNAKSGLLGSSIKWNFTKFLVDRAGRVVARHAPTARPEGLAREIEALL"
     gene            complement(16423..17823)
                     /locus_tag="VN968_16955"
     CDS             complement(16423..17823)
                     /locus_tag="VN968_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXL30957.1"
                     /translation="MEDLWRLSAADIAALIRSKKVSAKQAASAALARLDAVNPSINAV
                     VDHGPEDVLAQAGTIDAAIARGEEVGPLAGVPVTVKVNIDQQGFATTNGLKLQRDVIA
                     KTNSPVVDNLRKAGAVILGRTNCPAFSYRWFTTNLVHGDTRNPRDASITPGGSSGGAG
                     AAVAAGIGHIAHGTDIAGSIRYPAYACGVHGLRPTVGRIAAFNASLPERPIGPQISAV
                     SGPLARTVGDLRMALAAMSGKDARDPWWVPAPLEGPPMPKRAALCLRPDGLETVAEVE
                     AAVTDAGKRLERAGWVVEEIEDTPPLREAADLQTKLWLGDGFEAQLEAAEREGDPGAL
                     ACLRGNRAKVSAFDAAAYSKALTRRATLAREWLAFFETYSVLLMPVSAELPFPDGLDL
                     RDDASFARVWRAQLPQIAIPFMGLPALTISTGLVGRVPVGVQLVSTRYREDLCLLAGE
                     AIEAGGTPSAPVDPVR"
     gene            complement(17908..18474)
                     /locus_tag="VN968_16960"
     CDS             complement(17908..18474)
                     /locus_tag="VN968_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXL30958.1"
                     /translation="MRKIIAALAFACIATSVSAETIAERAAPCLVCHGEKGQSETENT
                     PSLGAQQAPYALIQLFMFREKLRVFEPMNEMAKALSDDDLRTFSDFLATLPKPVPPAD
                     AGDPARLQRAQALAQQHRCNSCHNTDFSGKDNVPRIAGQREDYLAKTLREYKDNSRHG
                     YDATMAEVLQPVTPEQIADLAYFLARAR"
     gene            complement(18694..19962)
                     /locus_tag="VN968_16965"
     CDS             complement(18694..19962)
                     /locus_tag="VN968_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXL30959.1"
                     /translation="MMICSSAPRSALLAGAFVGLFTFGAVAQQPADPAPAATAAPALP
                     PGSPLIGRPASEAAAKLAPVAPPPIAAAPDKLPTAKLKVPAGFNIEVYAAGMANARSL
                     AEGDKGTVFVGSRLVDKVYAVVNKDGKRSVKVLASGLYRPNGVAFRNGTLYIAELSKV
                     SKIEKAEDVIDNPPKPTMIYDNLPKDEAHGWKFIGIGPDNKLYVPVGQPGNNVLHDDA
                     HGQIRRINLDGTGAEVVALGVRNSVGFDWHPETKQLYFTDNGRDWMSEDVPQDELNRV
                     TKVGEDFGAPYCYQGNISDPEFGWGHSCSEFTAPVGLMGPHAAALGMRFYTGSMFPKS
                     YRNAIFVARHGSWNRSKKFGGDVAVVKLNKDGTVKSVQPFITGFLEDNKYVGRPVDVL
                     PMKDGSMLVSDDWNGTIYRITYGKPKVAAR"
     gene            complement(20176..20433)
                     /locus_tag="VN968_16970"
     CDS             complement(20176..20433)
                     /locus_tag="VN968_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30960.1"
                     /translation="MPRYFFNTQIGDELISDPEGEELQDLDRAWEVARAMIRELLRTE
                     GAERGLLSAILEVTDDEGEVVLEFPFAEAMLDMIDGPFTKH"
     gene            20731..20811
                     /gene="pqqA"
                     /locus_tag="VN968_16975"
     CDS             20731..20811
                     /gene="pqqA"
                     /locus_tag="VN968_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HXL30961.1"
                     /translation="MAWKTPKIVEVPVGMEINMYACAARK"
     gene            20978..21907
                     /gene="pqqB"
                     /locus_tag="VN968_16980"
     CDS             20978..21907
                     /gene="pqqB"
                     /locus_tag="VN968_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HXL30962.1"
                     /translation="MLRVVVLGAAAGGGVPQWNCGCPVCRMARTGHPELQSTQASIAI
                     SADGDNWFLINASPDLRQQLIATPQLHPKAGKLRHSPISGVILTNGEIDAVAGLLSMR
                     EGWPFTIYAHPRVLSILNSNSIFNVLNEKNVPRRPIEVNETFEPTLPDGSPSGIEILP
                     FAVPGKSAWYLEGKDHPAGGDGAGDTLGLRVSDRATGKCLYFLAACAHVTDDLKSRLG
                     GASLIFFDGTVWRDDELIAAGLGNKTGQGMGHIAMSGTNGAIEALAGLDIDRKVFLHI
                     NNSNPALLHDSAEREIAERAGWQIPADGTEITL"
     gene            21928..22683
                     /gene="pqqC"
                     /locus_tag="VN968_16985"
     CDS             21928..22683
                     /gene="pqqC"
                     /locus_tag="VN968_16985"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HXL30963.1"
                     /translation="MTAFSRGGSAPLNSAEELEAALRHIGATRYHNLHPFHRLLHGGK
                     LNKGQVQAWALNRYYYQSTIPIKDAVVISRFRDRGMRLEWRHRIEDHDGDLGTEGGIE
                     RWLKLTEGLGLDSAYVESTEGILPATRFAVEAYVHFVRDRTPLEAIASSLTELFAPNL
                     HEERISGMLAHYDFVNPDIMSYFSRRMQQAPRDANFALDYVKTHANTPEQREAVCNAL
                     LFKTNVLWVQLDALYHAYVDGHVPPGAFVPKIN"
     gene            22749..23066
                     /gene="pqqD"
                     /locus_tag="VN968_16990"
     CDS             22749..23066
                     /gene="pqqD"
                     /locus_tag="VN968_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HXL30964.1"
                     /translation="MAGLRSRNIRVSEASRPVLPRHAKLKFDETRQVWVILAPERVLA
                     PDKIAVEVLQLCDGVRSVAAMIDLLAEKYTADRAAISTDVVAMLQDLADKGFLTEARE
                     KTS"
     gene            23063..24271
                     /gene="pqqE"
                     /locus_tag="VN968_16995"
     CDS             23063..24271
                     /gene="pqqE"
                     /locus_tag="VN968_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HXL30965.1"
                     /translation="MSPTLTDAGVAASDPSDGLAVLEARGSTAETFGIPLAVLAELTH
                     RCPLQCPYCSNPVELERGSSELTTAEWKKVLSELAEIGVLQIHFSGGEPTARKDLVEL
                     VQHASDVGLYSNLITSAVLLTREKLAALADAGLCHIQISFQGNEPVVADRVAGLKNAH
                     EKKIEVAKWTRELDLPLTVNAVMHRQNLHQLSDIIRMAVDLDADRLEVANVQYYGWAL
                     KNRAALMPTLAQIEETSRIVEEAEARLKGILAIDYVVPDYYALRPKKCMGGWGRQFFN
                     ISPAGKILPCHAAETITGLEFLSVRSNHSIAWIWQNSEAFNRYRGTGWMPEPCKSCEF
                     REIDFGGCRCQAFALTGDAANTDPACTLSPLHEQIFKLAEQEAARDNKRFIYRNFAGG
                     TLETGGDHGA"
     gene            24261..25250
                     /gene="pip"
                     /locus_tag="VN968_17000"
     CDS             24261..25250
                     /gene="pip"
                     /locus_tag="VN968_17000"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HXL30966.1"
                     /translation="MAPDADAGKASGNRSDPFAPLTSQLLPVGDGHEIYVEAVGRADG
                     IPAVYLHGGPGSGCQPDHRRLFDPERFHAVLFDQRGAGRSRPKGHREHNTLPHLIADM
                     EIIREKFGFERWMIVGGSWGATLALAYAQAHPDRVTGIVLRATFLGTRAELEGAFLDA
                     LPRFYPDLSDDFLKLLPPEERAQPLDAYWRRILDSDPAVHGPAAHAWGDTERILSGHV
                     SGRVRLDSHSPNSTRALPATPFMEAHYFQNNCFMRPGQLLAEASKLAGSPAIIVQGRY
                     DLLCPPSMSHALAAVWAGSEVRAVEGAGHQLYDPGVRDAVMKAIADLASKITR"
     gene            25264..25941
                     /locus_tag="VN968_17005"
     CDS             25264..25941
                     /locus_tag="VN968_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30967.1"
                     /translation="MPLAGKGMLLTSMDIDPSDEAEFNRWYDREHLLERVAIDGFLEA
                     RRYIAHQGSPKYLCLYSTATFEVLDSPTYRTVLMNPTDWSKATLARFKNMIRAIARIT
                     VSRGQGRGAALGIVRLRPRAGDKDELRTALRDRLDPEKLDGIISMHLIESDPALSRPI
                     TDDPPVSDPGSGDWFVLIDATDVRAVSAAITDRLGDVPGQMAAETISTGVYKLMWDLA
                     KSDIRSS"
     gene            26236..28035
                     /locus_tag="VN968_17010"
     CDS             26236..28035
                     /locus_tag="VN968_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL30968.1"
                     /translation="MKPTDISAPDYFHKVVDCQWACPAHTPVPEYIRLIAEGRYSDAY
                     MINWRSNVFPGILGRTCDRPCEPACRRGRVEETPVAICRLKRVAADFKDDVKHRMPRP
                     SPKNGKRIAIVGGGPASLTVARDLAPLGYHCTVFDSDPKAGGMMRTQIPKFRLPDSVI
                     DEETDYILNLGIEFKGGTRIDSLKKLLGEKFDAIFVGSGAPRGRELEIPGRKEAASHI
                     HIGIDWLSSVSFGHVEKIGKRVIVLGGGNTAMDCCRTARRLGGEDVKVIVRSGFEEMK
                     ASPWEKEDALHEDIPILNFMVPKAFTHNNGKLTGVTFQKVKAEYDDKGRRNLVPAGDP
                     DQTIACDDVLVAVGQENAFPWIERDCGVEFDKWNMPKVDAKTMGSTNPKVFFGGDAAF
                     GPKNIIWAVAHGHDAALSIHKLLSGEDIAERPLPDVKISSQKMGIHEWSYDNDVSADK
                     RFKVPHRDKIIALKDIRSEVELGYDVKLALGEAQRCLNCDIQTVFAAPLCIECDACVD
                     ICPMDCISFTENGEEADLRTRLKAPSPHLDQDLYVADGLKTGRVMVKDEDVCLHCGLC
                     AERCPTGAWDMQKYLIDMTHAGSSCQSKSRSAA"
     gene            28005..29855
                     /locus_tag="VN968_17015"
     CDS             28005..29855
                     /locus_tag="VN968_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXL30969.1"
                     /translation="MPVKEPISSVNDFVVRFANVNGSGSASANELFARSILRHGVPVS
                     PRNVFPSNIQGLPTWYEVRVTEAGHLGARGGGVDMMVAMNPQTWDKDLASIEPGGYLF
                     YDSTKPMPTSKFRTDVTVIGVPLTAITNSTYTDPRQRQLFKNIIYLGAMCALLDMDPK
                     LVEQLIGEQYKGKEKLLSSNIHALHLGRDWALQNLKCPIGLRVKKSDKVGDRIFIEGN
                     SAAALGAVYGGATVCAWYPITPSSSLADAFGSHCKKLRHDPKTGKAKYAIVQGEDELA
                     SIGIVIGASWNGARAFTATSGPGISLMQEFIGLSYFAEIPAVIMNVQRAGPSTGMPTR
                     TQQCDILACAYASHGDTKHVLLFPEDPAEAFEFAAQAFDLAERLQTTIFVMLDLDIGM
                     NHRLSRPLKWDDARQYDRGKVMTAEMLDDGRDFGRYLDIDGDGIAYRTYPGTHPTKGS
                     FFTRGTSRDRYARYSEEGAVYADNMQRLVRKFEGAQDMVPRPLQANAAKLTKYGVIYF
                     GSTAPAMDEAIGLLEARGHLLDRLRIRAFPFHSSVASFIADHDFVYVVEQNRDAQLRS
                     LIVNENGIDPVRLIPIVHYDGTPITARFIAGAIGGHQDQLKLTPLRKVKS"
     gene            29852..>30858
                     /locus_tag="VN968_17020"
     CDS             29852..>30858
                     /locus_tag="VN968_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HXL30970.1"
                     /translation="MTYIVKPKFQHPGLPKNDLGFTHRDYEGKISTLCAGCGHDSITA
                     SIIEACFELSIEPHRVAKISGIGCSSKTPDYFLGSSHGFNSVHGRMPSVLTGANLANR
                     DLIYLGVSGDGDSASIGFGQFAHSIRRGVNMTYIVENNGVYGLTKGQFSATADRGSKN
                     KRGVMNTDNAIDLVAIALQLGASFVARSFSGDKTQLVPLIAAAIRHKGASFIDVISPC
                     VAFNNHAGSTKSFDYVREHNDAVNRLDVITGRAPIKVDYAPGSVQIVEQHDGSKLVLR
                     KLDSDYDVHDRVAAMGFLQTHAAKGQIVTGLLYVDGEPEDLHHHLNTVDTALNALSEK
                     EL"
BASE COUNT         6065 a         9667 c         9284 g         5842 t
ORIGIN      
        1 gttgatgccc ttcatgtgaa aggtcttgat gaagcccgcc ggcagatgga tgtagggatg
       61 ccaatcgcgg ggacctgctt cgagcacgca gatgctggtg atcgcatctt cgctcagccg
      121 attggcgagc acgcacccgg aggagcccgc gcccacgatc acatagtcga atctatccgt
      181 catcttaagg ttctggcggt atgctaccgc ggcttttcat tgagttgata gttcaaatgc
      241 gccttcaccg tcggccattc gccggcgatg atactgtaaa ccacggtgtc gcgcagggtc
      301 ccgtttggcg cgatctgatg gttgcgcaga atgccatcaa gcttggcgcc gagccgctcg
      361 atgccgcgcc ggctctggtg gttgaaaaaa tgcgtgcgga actcgaccgc gatgcaatcg
      421 agcttttcga acgcatgtga gagcaacaac agcttgcact gggtattgag ggcgctgcgc
      481 tgcacgcgct tggcatacca ggtcgacccg atctcgacgc gccggttcgg cgcatcgacg
      541 ttcatatagg tcgtcatgcc agcgatgttg ccgtcggcgt cgaagaccgt aaacggcagc
      601 atcgaccccg ccgcctgcag cccgagcctc cgatcgatct ccttgcgcat gtcttcggcc
      661 cgggggatga aggtgtacca gagcttccac agctcgccgt ccttcaccgc ctcggtcagc
      721 ccgtcgcaat ggtcctgcga caagggtttc agctgcgcgt gcgggccgct gagcgatatc
      781 ggttcaagcc agggcattaa atactccgct cgttaggccg tcattgcgag gagcgaagcg
      841 acgaagcaat ccatctttct tgtccgtggc cttatggatt gcttcgcttc gctcgcagtg
      901 acgcatccta aatcacttgt tcaaaaaatt cagcggcagg ccgggccgcg cccagtccat
      961 cgcgacgagt tggcccttgc cggacagcgt gatgtaggcg gtcttgagct ccggcccgcc
     1021 gaaggcgatg ttggtggtga cgcggtcgcc ggtcggcacc tgttcgacga tggcgccgtc
     1081 cggcgcgatc accgaaatgc agcccgagat caaggtcgcc acgcagacat tgccggaagc
     1141 ttccaccgcc agcgaatcga acatctggta gccgccgagg cccaccatcg gcttgccgcg
     1201 ttcaccgcga tagatcacgt cgcgcggctt gatctcgccg ggcgcggaca ggtcgaacgc
     1261 ccacagccgc gcggtcggcg tctccgcgac atagaccgtg ttctcatccg gcgacagccc
     1321 gatgccgttg gtcggcatca tgccgaacac ggcttcgacg acctccgtgg ccccgggctt
     1381 catgtaatag aacgcgccga catccatgtc gcgggcgcgg cgcttgccga gatcggtgaa
     1441 ccagagtccg ccatgcttgt cgaacaccag atcgttcggt cccttcaggg aatgcttgcc
     1501 gcatttgtcg aacagggttt cgacctcgcc ggtcgcaaga tcgacgcgct ggatcgagcc
     1561 gccgatatat tcatgaggtt cgggcgcacc cggcatcatg gtaccccgcg acgggatcca
     1621 gctgaaaccg ccattgttgc agatgtagat cttgccgtcg gggcccatcg ccgcgccatt
     1681 ggggccgccc ggaatttccg cgaccacctg tttgcggcca tcgggccaca cccgtgtcag
     1741 ccgttgccct cgaatttcga ccagcaccac cgatccgtcc ggcagcacca ccggcccctc
     1801 ggggaactca agatcggtgg cgagaacgcg gacatcgggc atcggaatcc tccggctttt
     1861 cttaaggcgc cggcggattc ccaagcctat tccgcacgcg caacgatttc attacgcgct
     1921 gttatggcaa agccgcgcgc gcttgccaag caatcggcgg ccaggggccg cgcaaggcag
     1981 ccccgcgtgc ggcccagagt ccttattcga tgcgttttct tcgcgccaac cggggcaggc
     2041 tttcgctcgc taacgccttt actccctgac cggcacccaa atctccagcc cgccattgcc
     2101 ggtgagcgga tcgaagtttt cgtcgtagcg ctcgaagttg ggagcgtcgg ctgctttcag
     2161 gcccgaggcc ggcagccagt ggttccagat cgtgttgacg gtgcggcgaa tggtcgagat
     2221 gtggtcgctg tgcgtgaaga ccgcgtatct ctgttcggga atccggacgc tatggaattc
     2281 gcgcggcagg tcggagaagt cggatacttc gacgccggcg atataatcaa agtttccggc
     2341 gtcgtcgccg ttgcagcaga cgccgtatgc caccttcccg atccggccgg gaatgttctc
     2401 gacggcttga tgaaagcgct gccattgatt gggaatgccg gcgccgttct catggttata
     2461 gcgctcgctg ataccggcga cgagcagcgg cctgctggtt tcaaaacgcg gcgcgtgaag
     2521 cttgcctgtg agggttgaat ccatttgaat cggctcctga agcttgatgc catcgaggca
     2581 cgtcgatgca cggaccgctt cgggcgtgac cccgaaatgg ccgcggaacg cgcgggtgaa
     2641 tgcttcgtga gagccgtaat ccgcgtccag cgcgagggtg aggatatcgg gtgcgccatt
     2701 tgccagcgcg cgcgccgcct cggtgaggcg ccgggcgcgc acatagcgca tgaccgataa
     2761 ccccgtcgcg gcggcgaacg cgcggaccat gtggaagcgc gagactccac cgatcgctgc
     2821 aatctcatcg agggtcaacg cgtcggcaag atggctttcg ataaaccaga gcgccttctg
     2881 ggccggattc atggacgaca gccttcgttc gaagctcgaa catcatgatc gatttgcgcg
     2941 cgcttcattt gacagtcctt gctctcatgc gtcgtttgga agctggaacc ggacacgatg
     3001 atttctgccg ctccgcggaa aataccagcg aaggcgtcgc tggcaacgtc attccaatcg
     3061 tgcatactgc gcctgcattc aatccgacgg ctcatcaagc tgcccaccac gaagacggcc
     3121 tgagcctcgg ccagcgcgtc agggaggacg atgatgcact accgtgtctt gagccatgcc
     3181 agatccgtag gccttgccgg actgcttgcg cttgcggtcg caagcgccgc caacgcgcag
     3241 aagaaatacg atccgggtgc taccgatacc gagatcaaga tcggcaacat catgccctat
     3301 tcgggaccgg cgtcggctta cgccaccatc ggcaagaccg aggcggccta tttcaacaag
     3361 atcaatgccg acggcggcat caacggccgc aagatcaatt tcatctccta tgacgacggc
     3421 tacagcccgc cgaaggcggt ggagcaggcg cgcaaattgg tcgaggccga cgaggtgctg
     3481 ctgattttca atccgttggg cacgcccagc aacagcgcga tccagaaata catgaacgcg
     3541 aaaaaggtgc cgcagatctt tgtatcgagc ggtgcggcca aatggaacga tccgaagaat
     3601 tttccgtgga cgatcggctg gcagccgagc taccaggtcg aggcacgcat ctatgctgcc
     3661 tacatcctga aaaatcaccc cggcaagacc atcggcgttc tctatcagaa cgacgatttc
     3721 ggcaaagact atgtgatcgg gctgcgcgaa ggcctaggcg atcagggcaa caagctgatc
     3781 gtggtcgaaa gttcatatga gaccacctcg ccgacggtgg attcgcaagt cgtgcagatc
     3841 aagggtgcta atcccgacat cttcgtcaat gtctcgacgc cgaaatttgc cgcgcaggcg
     3901 atcaagaaaa tcggggaact ggcctggcac cccgtgcagt tcctcaccaa cgtgtcggct
     3961 tccgtcggcg gcgtgatgaa gccggccggt tacgaaaacg atcaggacat cctcagcgcg
     4021 gcctgtctga aggacccgca ggatcccaaa tggaaagacg atccggccat gaacgaatgg
     4081 cgggccttca tgaccaagtg gtatcccgaa ggcgaccagg ccgacgcagc gaccacgttc
     4141 ggttacggcg tggccaaggg gctggaacag gtgctgcggc agtgcggtga cgatctcacc
     4201 cgcgaaaaca tcatgaggca ggcagccaat ctgaatttcg aaatcggcgt gtatctgccg
     4261 ggcacgcata tcaagaccag cccgaccgac ttcgcgccgc tcgagcagct gcagatgatg
     4321 cgcttcaagg gcgagagctg ggatctgttc gggccgatca tgtcaggcga aaagaacagc
     4381 tgagtttctt tcctcggtcc ccttgtcgga gagggtgcct tcgcgaagcg aaggcgggtg
     4441 aggggctgac tcctcgttct gaagccaccc ctcacccgtc tcaaactcgc ttcgctcgtt
     4501 ctcgccaccc tctccgacaa ggggaccgac aaggggagag ggaagcaagg cggccgtttc
     4561 atttcgcgcc atccttcgca tccaccggca tccgtgcatt atccttggcc ggcggtgcgt
     4621 cggctttctt ctgatcctcg agcgctttct tctgcgccgc ctgcaggtcg tccacgatct
     4681 tttgcaagga ggcgacggta gctttcaaac catcgatctg ccgggtcgcg gcatcgagtt
     4741 tctgctgatg gtcggcggcg agttttgtga tcgtcttttg caggaagtcg acattgctct
     4801 gcaggcaact ggtccgccgc tccatggttt tttcgacggt gcagatttcg atgccgggaa
     4861 cgtcctgggc acgcgcatcg accggtacca gcacgcagga tgtcaaaacc actgacgcga
     4921 cgagagcgat gtggccgatc atggatatcc tcacttgaag catttttccg gctaagtgga
     4981 atccgattgg cggcaagaaa atgctcctga ccaataaatt gcgcgtattt tgccgcagat
     5041 cgcttcacgc aacctgcgtt aacttgattg cggagcaaaa cggtagacac cgttttgcgg
     5101 aatccgcgat aagtacttct catcacatca aattgcgcgg agccggtgcg cgacgggaac
     5161 cataccacac tgaaacctca ttcgcgcggt gaggtttgca attaataccc aagctaaggc
     5221 agggggcacg gcttgagggc gcggaacgca gacgcggtgc ttgcgcctct ctttcaggga
     5281 gaaatacgga aatggcaacg cgcgaacgac gtctggccga attcaacggc aacggtgaac
     5341 cgccgccgga tcagccggtt caggtgcttt gcgaggacca cagcggcacg tatcaacttc
     5401 cgttcgcctg ccgcttcgtc ggcggcgaat ggcgcaacaa cgagtccggc gggaccgtgg
     5461 aggcaaccgt ggtcggctgg cgcctgcctc gcacagccag ccgcgcgctt tagcctcggc
     5521 cgctgtgcca taatgcaacg gagcccggcg gcgttttaag attcagaacg ccgacggaac
     5581 gaaccgcgcc cgaatcagtg tttccgccgc gtactctcat gttcacggct agatgtcggt
     5641 ctgaatgtca gacggcgcac gacatccagc accacggcga tggagcgcag ccaacgaccg
     5701 acgacgaatg ttaattcctc cggcgacgac acttggccta cttccagggc gtcaccggcg
     5761 gcaccttgtc ggtggccggc ggcacgtcga cggttttgaa atccgccggg ctgacgattt
     5821 cgagatattc catgtcgggc gagtaatcgt agaggtagtg cacgatccct ggccgctgat
     5881 ggacgacgtc gccggcctga accagggtcg acttgtcctc gtacatgaag cgggcccagc
     5941 ccttggtcat gatgacgatc tggaaatcgc attcgtgttt atgccagccg gtaccgtctt
     6001 ccggcggcat ctcgggattg gccttgacca gatgacagat cacctgcccg ccggtcgcag
     6061 cggcgatgcc aagatcgcgg tacaggaaga agtcgcgcaa gccgcctccg gtaaattcgg
     6121 tatcaccggg tttgacgtgg gaaaatgtgt tgacgacagc gtgctgattc atcagggcct
     6181 ccactgttct tgttcaagca atcgcgacaa gcccaggcgt ccaatttgcg tccaattttt
     6241 tggcagcacg ggattgccgt tttcgcggct tcattttgct atgcgaaaat cacgccagcg
     6301 gcgctgcaac gcggcgaatt caacggccgc tgcggctggc atgccgaagc gcttggacgg
     6361 aagcggaaat ccgggtcgag cgatttttgg cgcaccactt ccggcccggt cctgccgatt
     6421 gccgaaaacg ctctggatgg caccaagtta agcaaggcct gcccgatgct taagcccttc
     6481 caagaggcag cgggaagccc gcgttgccta atattcgacc tcaagctctt cgatatcggc
     6541 gggctcgacc ttggcggcct cgatccattc ctgcatctcg ggcaacgcca tcaccatgtc
     6601 ggcataggct ttcagcctcg gctccagctt cacatcatag gtcatgaagc gggtcacaac
     6661 cggcgcatac atggcgtccg ccatggtccg gcgttcgccg aacaggaacg ggccgcccga
     6721 tttggcgagg cattcgcgcc agatcgcgca gacccgatcg atgtcggctt gcgcgcgcga
     6781 ccagatcttg aagcccggga aatggccttt cagattgacc ggcagcgacg cgcgcagggt
     6841 ggtaaatccc gaatggattt cgccgcaaat cgaacggcaa tgtgcccgca agacgcggtc
     6901 cgccggcaac aggccggcgt ccggcatgac ctcgttgagg tactccgcga tcgccagcgt
     6961 atcccacacg gtggcgccgt cgtgccgcag acatggcacc aggatcgatg acgacagcag
     7021 caggatttcg gctcgcgccg aagcatcgtc cggcgcggtg atgatttcct cgaactccag
     7081 cccggagaac ttcgtcagca accagccgcg taacgaccac gacgaatagt tcttgctgct
     7141 gatggtcagt gtcgctttcg ccatatggcc tttccctcac gcctgaacgc ccgccagggc
     7201 cgccgcgcca tgcgcactgt ttatgctcga agcgtttatt cagcaagcga cgtgccagtt
     7261 tgcgggatta attgactccg ctttggcttc attattgcat agcaacaaag ccaggggcga
     7321 acccggatga tgatgatgta ccaggcgtat cagaaccaca tggacctcac ggcgccatgg
     7381 cggaccgggg ctgcatcggc gctgaaatac cttaacctgg tgccgcaggg cgtctccgac
     7441 aaactgttcg ggcggctcgc cgcggcgttg gagttgatct cgcgcacctc gctcacctac
     7501 acgcggccgc cctacggcat cgacaaggtg ctggtcggca atcgggaact cgaggtgacc
     7561 gaggaagtcg cgtacgccac gccgttcggt tcattgctgc atttcaagaa gcaaaactca
     7621 ccggagcaac cgcgcatgct gctggtggcg ccgatgtccg gccacttcgc aacactgctg
     7681 cgcggcacgg tgaaaacctt gctgcaggat catgacgtct acatcaccga ttggcataac
     7741 ccccgcgaca ttccgctcga tcagggcagg ttcgggctcg acgaatacac cgagcacctg
     7801 atcacgtttc tcggtcaatt gggcccgcgt gcgcacatgg tggcgatctg ccagccgtcg
     7861 gtgtcggcgc tcgccgccgc ggcgatcatg tcggaggaca atcatccggc gcggccggcg
     7921 accctgacct tgatggcggg cccgatcgac acccgaatcc agccgaccaa ggtcaacgat
     7981 ttcgccaaga gcaaaccgct caaatggttc gaggagaacc tgatcaacta tgtgccgttt
     8041 cagtgcaagg gcgcgttccg gcaggtctat cccggattca ttcaactcac cgccttcgtc
     8101 tcgatgaacc tcgagcgtca catcaaatcc cacgtcgatc tcgccgatca tttggcgaag
     8161 ggcgagaagg aaaaggccga aaccatcaag accttctatg acgaatattt cgcggtgatg
     8221 gacctgtcgg cggattttta tatcgagacc atccgcgacg tgtttcatga acatctcttg
     8281 ccgctgggca agctgaccta tcgcggccgg ccggtgagcc cggccgcgat caagcgcatg
     8341 ggcctgatga cggtcgaagg cgagaaagac gatatctgct cgatcggcca gacgctcgcg
     8401 gcgcaggacc tctgcaccgg cgtgcgcgcc tatcgcaagg tgcatcacat gcaggccggc
     8461 gtcggccatt acggcgtatt cagcggcagg cgctggaaca acgaaatcta tccgctgctg
     8521 cgggatttca tccacgtcaa ttcgtaagct cagtcattcc ggggcgcgcg tgaaacgcgc
     8581 aagcgcggaa tcccgcagaa acgatacgtc acttcgggat tccggatcgg tccgcgtcgc
     8641 ggaccgtccg gaatgacgga gaattggaga attagagagc agccctattc tgtcctccgt
     8701 ttggaggata cgatcgtctc aacgacgacc agtcaagagt cgtcccggga gactgatcat
     8761 gaaatcgata tccgcaaata tccgcagcct cgttatcggc gccattggcg ccctgctgct
     8821 ggcaggctcg gcgaacgccg ccgaggtcag ggtgatgatc tcgggcggac tgacggcggc
     8881 ctataaggag ctggtaccgg aattcgagcg cctgaccggc aacaaggtgc tgacggccta
     8941 cgggccctcg atggggacca ccgtcaatgc gatcccggtc cggctcgagc gcggcgagcc
     9001 cgccgatgtc ctgatcatgg tcggctatgc gctgggtgat ctcgtcaagc agggcaaggt
     9061 gatcgccgac agccgtgtcg atctggtgaa atcgccgatc ggcattgcgg tgaagtcggg
     9121 cgcgccgaag ccggacatca gttcagccga cgcggtgaag cgcgcgttgc tggcggcgaa
     9181 atcgattgcc tattccgact cagccagcgg cgtctatgtc tcgaccgaga tgttcgaaaa
     9241 gctcggcgtt gccgaagcga tgaaggacaa ggccaggaaa attcctgcca ccccggtcgg
     9301 cgaaatcgtc gcgcacggcg acgccgagat cggtctccag caaatcagcg agctcaagcc
     9361 cgtagaaggc attgatatcg tcggtcccct ccccgacgac ttgcagaaga tcacggtttt
     9421 ctcggccggg attgcgaccg tgtccaggga gcccgacgcc ggcaaggcgc tgatcaaatt
     9481 tcttgcctcg cccgccgcca gcgcggtgat cgtcagaagc ggcctggagc cgatcccggt
     9541 tggtgcaacg aattagcagg gcttgacacc acgcgctatt ttacccggat cggtgcgtcc
     9601 ctcagtccat tgttgtcgta ggcgcgtcgc agcatgccgt tcgagattgc gtccgtcatg
     9661 aacttgttca cgaaggccag cgccagcgga tgatcgagcg gtaccgccac cgcggtcacg
     9721 gtctgcttga aggtttcatc cagtacgcgc gttccgggaa tcttttttgc catatcgttg
     9781 agctggtcgc gcgacagcgc aaatgcatcg atctcgccgt tcttgagcaa cgcaaatatc
     9841 tcatcgtagg tctgatactc gatcactttc gcattcttta gatgcgcgac cgcgccgcgc
     9901 atggtggtgg tgttatattc tgccgcgacc ttaatgccgg gctgatcgag cgtctgaaaa
     9961 ttcgtcaccg acgagcccgc cttgacgatg taggtcgcat ccgtgacctg atagatcggc
    10021 ccgaatccca tcttggtcat gcgcacggcg tcctgcggca ggaacgtcac atcccaaatg
    10081 ccttttgccg cagcgtcggt gatctgtccg gaattgtcgt acgcgacata ttcgacaggg
    10141 acggcgagtt gcgccgccat ctccttgccg agatcgaccg gcaccccggc atacccgccg
    10201 gcctcgttct tggtcgacca gaacgcgccg cccgcggtgc tgatcgcaat cgccacccgc
    10261 agcttgccgg tcggcgcgat ttcgtccttg agggcatcgg cgcttgcggg aaccgccatc
    10321 atgattgaaa cacccagaac gaggccggcc aggcgcggcc aggattgatg ggacatccga
    10381 atttctccac ccatgggctc gttatggccg agccttcgcc ggccatagtg gtcgattgcg
    10441 ggaggcgttg aaacccggat tctccggcgg gatcgataaa atttattggc agtaccccgg
    10501 gccggatcaa gcgccgtgcc ggcacgtcaa gacccgccag cggcgatggt cgcgcaaaat
    10561 tcggctgctt ctgcacgttg ccggaatcag ttcgcgaccg ccggcacctt gatctcctgc
    10621 ggcaggatga tcgcggccgc gatcaccagc agacacagcg cgccgaaagc atgcagcatg
    10681 atgacaaacc ctccctgttc gtacagccac gccaccaggc cgaccgatac cccggcggcg
    10741 gtgaagccga tgaaatagcg caccgcatag gcgcgcgagc gccattcctc gctggtgtat
    10801 ttgccgacca tggcgtcgtt gacggtgacc tgcccgaacg cccccatcac gatgccgatc
    10861 gaagccagga tcagcggcag gttggacaat gtgcccgcca gatacaggaa cggcgccagc
    10921 atgaaggaca gcggcaacat gacggtcttc agcgaataac ggtcgatcag cttgccgatg
    10981 gtgtattggg tcatcgcccc gaacacatag acgcaagccg cgatcacacc gagcagggcc
    11041 gggcttttgg tcaggtcgct caagcgctcc gcgaacaatt ttggcagcgc caccgtcacc
    11101 gcgttgaagg tagtcgagac cgcgatcacg acgatcagga gcgcaacgaa cacccgccac
    11161 atttcgttct ttgcgacccg ggcctgcgcc gccgcctgtt tgaagccgga gcgatcctcg
    11221 tggaccaccg ttcgcgcaaa ggccaagccg atcaggatgg tgacgatgcc gggaatgata
    11281 aaggcccagc gccagccgag gcactggccg accacaccgg tcaccagcgc cgacgacgcc
    11341 acgccgagat tgccccagac accgttgagg cccatctccc ggccgagcct gtcggcgtag
    11401 gacacgatca tcgcggtgcc gacggggtgg tagatcgacg cgaacaggcc gatcgcgagc
    11461 agcgccgcgc caagctgcag cggcgtctgc acgagaccaa ccgcaatcat cgatgcccct
    11521 ataccggcaa agaaaatcac catcatgtgg cggcggctcc agcgatcccc gagccagccg
    11581 gtcagcagcg atccggcgcc gaacgcgacg aagcccgggg tggcataagg cagcagctcc
    11641 gaataggcca tgccgagcgc cggccccatc acgatgacag cggcggcgaa gatcagcatc
    11701 gaataatggt cgataaaatg ggcggcgttg acgaaaccga tcaccctgtt agggctgttc
    11761 atagacttgg tcccttgatt tcctgaaata ggttataggg ccttgtcctg acgggatgcg
    11821 gccaatgaac atcgccgaaa cgccaatcgc cgccatccgg ggcagtcacc gcgccaccgg
    11881 ggacggcgtc catctggtgg cgcgccatta ccgcaagggc gtccggctcg acactcacat
    11941 gcaccgcgaa gcgcaactgg tgtacgccgc caagggcacg atgcaggtca cgacgccaaa
    12001 ggggcgctgg ctggtgccgc ccgaccgcgc ggtctgggta cccgcccgtc tcgaacattc
    12061 gatcgacgtg ctggcggata tcgagatgcg gacgctgtat ttcgatctgg catggctggc
    12121 gcgcgaggac cgcagcgaga gcctggattg cgagttcgtc gtccgggtct cgcggctgct
    12181 gcacgaaaca atcctcgcgc tgttcgatgg ccagggtaac cctgaccgca ccggacttct
    12241 ggtcaagctg gccgtcctcg aactgcacca cgccgaggat tccgcgacgt tcattccgct
    12301 gccgcaggag ccgcgctgcc ggcgtgcggc ggatatcgtg cttggcgacc ccacggccgc
    12361 gcatgagatc gagacgctgg cgcgcgaggt cggcacgtcg gcgcggacgc tgtcccggct
    12421 gttttcatcg gagacgcaac tcagcttcaa gagctggtgc caacgcgcgc gcatcgcggc
    12481 ggcgatcgaa cgattgtcga cgcaggccga tgtctcggtc aaacagctcg cctcggagct
    12541 cggctatgcg agtgtgccgg cgttttcaca cgcattccgg caggtcaccg gcaagacgcc
    12601 gacgcagttt gcggagacga aataagaccg ccgcgaacga ttttgcctgt catacgcggg
    12661 ctcgacccgc gtatccatca gtcttcatga aaatgtcttt caagagggat ggattgccgg
    12721 atcaagcctg gtaacgacgg tctgtggccg gagtgcaagg cggttttcta cggctcccaa
    12781 taatgtatgc ttgattgtga tcggctccgc cttaaattgc atgtatgact tcacctgaat
    12841 tggatttgac ccgcccgaca tgagcagcaa cgccttcttt tcagatcttc tcgcgtcaat
    12901 ttccgagcgc ggccgcacgt tgctacgccg cgcggggtca tccgacacca agcggcacgc
    12961 ctccgacctg atcgaactgt gcgaggcgct gttgtccggg cgaggcgagg cctccggcac
    13021 ggcgatggcg cgcgaggtcc tcgaccatta ccatagtctc gatgaggccg gccggcttgc
    13081 attctttgaa accctgaccc gggacttcgg tccggaccgg gaaaagcttg ccgaggcgat
    13141 cgaggcttgg cgcgcacaac cgaccgacgc cgacgccagc gatctccact ttgcctccga
    13201 gccgcggcgg caggaattga tccgccagct gaaccgcgcg ccgggcggca ccagcgcgct
    13261 ggtcgccatg cgcgccgatc tgcttggcgc catgaacggc cacaaggacc ttgccgcgct
    13321 cgatcgcgac gtcgtgcatc tgttgtcgtc ttggttcaac agggggtttc tcgtgctgcg
    13381 caggatagac tggtcgacgc ccgccaacat cctggagcag atcatccgct acgaggcggt
    13441 gcatgagatc cgcgactggg acgatctgcg ccgccgtatc gatccgatcg accggcgctg
    13501 ctatgcgttc ttccatcccg ctttggtcga cgagcccctg atcttcgtcg aggtggcatt
    13561 gacggaatcg attcccgccg cgatcgcgcc gctgctcgcc gaagaccggc agccggtgcc
    13621 ggtcgagcgc gcccgcaccg ccgtgttcta ttcaatctcc aacacgcaac gcggtctcgg
    13681 cggaatttcc ttcggcaact tcctgatcaa gcaggtggtc gaggaattgc ggcgcgaatt
    13741 gccgaagctg gagaatttcg tgacgctgtc gccggtgccg ggcttcatga aatggctgaa
    13801 gcaggcgagc gatgttccgg tgtccgatga agagcggtca cagctcgctc atcttgacca
    13861 gcccgactgg ttcgagcacg ccgagctgac gtcgcaactg cgctcgctga tcgagccctt
    13921 ggctgcgcat tatttcctga aagcgcgcac ctcgaaaggc cggctgatcg attcggtggc
    13981 gcggtttcat ctcggcaacg gcgcacggct ggaacggatc gactggcttg gcgatctctc
    14041 gccaaagggc atgcgcgaat ccgccggcat catggtcaac tatctctatc gtctcgagga
    14101 catcgagaag aaccacgaag cctacgccaa ccagggcgaa atcgccgctt ccagtgccgt
    14161 gaaaaaactg ctgaagaccg aaggccggcg gctattggat atgcggctgt cgtagcgcag
    14221 accatcgcca cctgccgtca ttccgggatg gtccgaagga ccagacccgg aatctcgaga
    14281 ttccgggttc gcgttttcaa cgcgccccgg aatgactcct ccctaattca cgacgccagc
    14341 gccttcatct ccgcgtaaag atcggacttt ccttcaaaac cgatgcccgg caaatccggc
    14401 atcgtaatgt gaccgttctc gacgcgcacg ccatccggaa agccgccata gggctggaac
    14461 aggtcgggat agctctcatt gccgccgagg cccagccccg ctgcgatatt cagcgacatc
    14521 tgatggccgc catgcgggat gcatcggctc gcggaccagc cctgcgtttg cagtacggca
    14581 agcgtgcgct gatattcgca gagcccatag gacagcgcgc aatcgaactg cagccagtcg
    14641 cggtcgggcc gcatcccacc gtagcgaatg aggttgcgcg cgtcctgatg gctgaacagg
    14701 ttttcgccgg tcgccatcgg cccgggatag aattccgcca gcgccgcctg caactggaaa
    14761 tcaaggggat cgccggcctc ctcgtaccag aacagcgtat agtcgcgcag catttttgca
    14821 taggcgatcg cggtctccag atcgaaccgg ccattggcat cgacggcgag ttgcgcatgg
    14881 ctgccgattt cctcaagcac ggcctcgata cgcatgcggt cttcggcgat cggcgcgccg
    14941 ccgatcttca tcttgacgac attgtagcca cgatcgagat agccccgcat ctcgccgcgg
    15001 agcatcgaca ggcccttgcc cggataatag tagccgccgg cggcatagac gaacacccgc
    15061 gggtcggcgt tgcgtccgtg acgctcggcg agcaatcgaa acagcggttt tcgtgcgatc
    15121 ttggccacgg catcccacac cgccatgtcg atagtgccga ccgcgaccga gcgctcgccg
    15181 tgaccgccgg gcttttcgtt cgacatcagc gtcgcccaga ccttgtccgg atcgagattg
    15241 tcgctcgttt catcgagcag ggtcttcgga tcggcttcca tcaaccgcgg cgcgaaacgc
    15301 tcgcggatca gcccgccctg cccgtaacgg ccgttggagt tgaaaccgta gccgaccacc
    15361 ggtttgccgt cgtgcatcac atcggtgacc acggcgacga ggctggtcgt catcttcgaa
    15421 aaatcgatat aggcgttgcg gatcggcgag gagatcggct ttgtgatttc gcggacgtcg
    15481 acaatgcgga cagacatggt gcttggcttt ctacttcttc gtcatgcccg gccttgtgcc
    15541 gggcatccac gtctaaacca tgcaacagcg agagagacgt ggatggccgg gacaagcccg
    15601 gccatgacaa ctttttctgt tggctgcatt tcgagtcagc ctcaggatga cgccttgtcg
    15661 cggaagtaag gctctacggg accgtggact ttgatggtga gcgggttacc gtagcgatcc
    15721 ttggcgttgc ccgccgtgac ccgcacccag ccctcgctga cgcaatactc ctcgacgttg
    15781 gtcttctcga cgcccttgaa gcgaatgccg acatcgcgcg ccagcacgtc ggcattgtaa
    15841 taggggcttt tcggatcgct cgaaagacgg tccggcaact ggtcagtcaa ttggccggtc
    15901 atggcatcat tcttctcgtt cacaatagcg cctcgatttc tcttgccagt ccttccggcc
    15961 tcgcggtcgg cgcatgccgc gccaccaccc tgcccgcgcg atcgaccagg aatttggtaa
    16021 aattccattt gatcgacgag ccgagcaagc ccgacttcgc attcttcaga tagttaaaca
    16081 acgggtgcgc gcctgtgcca ttgacgtcga tcttggcaaa catcggaaaa gtgatggcat
    16141 agttgctggc gcagaactgc tcgatttgcc gggagtcgcc gggttcctgg cttccgaact
    16201 ggttacacgg aaacccgaga acggaaaagc cgcgcgggtt aagcgcctga tgcaacgtct
    16261 cgaggccctt gtattgcggg gtaaatccgc acgcgctcgc ggtgttgacg atcaggagca
    16321 cctgaccctc gaaccgcttc aggggaacgt cctcgccggc aagcgacttt gccgtgaaat
    16381 cgtgaacgcc gggcatcggg tttctttcag cttgaaaatt cactaacgaa cgggatcgac
    16441 cggcgccgat ggggttccgc cagcttcgat cgcctcgccg gccagcaaac acagatcctc
    16501 gcggtagcgg gtggagacga gttgaacgcc gacgggcaca cgccccacca gtcccgtcga
    16561 gatggttagc gcaggcagcc ccatgaaggg aatggcaatt tgcggcaatt gcgcccgcca
    16621 aaccctggcg aacgaggcgt cgtcgcgcag atcgaggccg tcggggaacg gcagctcggc
    16681 ggagaccggc atcagcaaca ccgaataggt ctcgaagaac gccagccatt cgcgcgccag
    16741 tgtcgcccgc cgtgtcagcg ccttcgaata cgcggccgca tcgaacgcag aaaccttggc
    16801 ccggttgccg cgcaagcacg ccagcgcgcc gggatcgccc tcacgttcgg cggcctccag
    16861 ttgcgcctca aaaccgtcgc cgagccacag cttggtctgc aggtccgcgg cttcccgcag
    16921 cggcggcgtg tcctcgatct cttcgaccac ccatcccgcg cgctccaggc gcttgccggc
    16981 atcggtgacg gccgcctcga cttcggctac ggtctcaaga ccgtcaggac gaagacacag
    17041 tgccgcccgc ttgggcattg gcggcccttc cagcggcgcc ggcacccacc aggggtcgcg
    17101 cgcgtcttta ccggacattg ccgccagcgc catccggagg tcgccgacgg tgcgcgcgag
    17161 cgggccggat accgcggaga tctgcggccc gatcgggcgt tccggcagcg acgcattgaa
    17221 ggctgcgata cgaccgacgg tcggccgcag accatgaacg ccgcaggcat aggcgggata
    17281 gcggatcgag cccgcgatat cggtgccatg cgcgatatgc ccgatgcccg cggcgaccgc
    17341 ggcacctgcg ccgccggacg agcctcccgg cgtgatgctc gcgtcgcgcg gattcctggt
    17401 atcgccatgg accagattgg tggtgaacca gcggtacgag aatgccgggc aattggtgcg
    17461 tccgaggatc accgcgccag ccttgcgcag attgtccacc accggactgt tggtcttggc
    17521 gatgacatcg cgctgcagtt tcaggccgtt ggtggtggca aacccctgct ggtcgatatt
    17581 gaccttgaca gtgaccggca cgcccgccag cgggcccacc tcttcgcccc gcgcgatcgc
    17641 ggcatcgatg gttcctgcct gcgccaacac gtcttccggg ccgtgatcga ccacggcgtt
    17701 gatcgagggg ttgaccgcgt ccaatcgcgc cagcgctgcc gatgcggcct gtttcgccga
    17761 aacttttttc gacctgatca gggcggcgat atccgcagcc gacagacgcc aaagatcttc
    17821 cattgccaac tccgtttgat tgcccgtctt ttagcgctgc ccacaggccg gcgccatcgg
    17881 gaaacaaccg tgtcagcgac tgcaagctca gcgcgcgcgg gcgagaaaat aggcaagatc
    17941 ggcgatctgc tccggcgtca ccggctgcag cacctccgcc atggtggcgt cgtagccgtg
    18001 ccggctgttg tccttgtatt cccgcaaggt cttggcgaga tagtcctcgc gctggccggc
    18061 aatgcgcggg acgttgtcct tgccggaaaa gtcggtgttg tggcaggaat tgcagcgatg
    18121 ctgctgcgcc agcgcctggg cgcgctgcag ccgcgccgga tcgccggcgt cggccggcgg
    18181 taccggcttc ggcaaggttg cgagaaaatc ggagaaggtg cgaaggtcgt cgtcgctgag
    18241 cgccttcgcc atctcgttca tcggctcgaa cacgcgcagc ttttcacgaa acatgaaaag
    18301 ctggatcagc gcgtaaggcg cctgctgcgc gccgagcgac ggggtatttt cggtttcgga
    18361 ttgtcccttt tcgccatgac agacaaggca cggggcggca cgctcggcga tcgtctcagc
    18421 gctgacggaa gttgcgatgc aggcgaacgc caaagccgcg attattttac gcatgacagg
    18481 tcccggcgac gacgatcaag aaattgaaac gttttcgtca ttgcgaggcg ccaacgggtc
    18541 cggccttcgg ccggcccgat gataaaactc cgcgacaaag caatccagct ttttggtgcg
    18601 gctatggatt gcctcgcgga gcctgtcatc gggcgcgcat tcgcgcgacc cgttggctcg
    18661 caatgacgaa ttcaagtttt gttgctaccg atcttaccgc gcagccacct tcggcttgcc
    18721 gtaggtgata cgatagatgg tgccgttcca gtcgtcggaa accagcatcg aaccgtcctt
    18781 catcggcagc acatcgaccg gccggccgac atatttgttg tcttccagga agccggtgat
    18841 gaacggctgc acggacttga ccgtgccgtc cttgttcaat ttgaccacgg cgacgtcgcc
    18901 gccaaatttc ttcgacctgt tccatgaacc atgccgcgcc acgaagatgg cgttcctgta
    18961 actctttgga aacatgctgc cggtataaaa ccgcataccg agcgcggccg catgcggacc
    19021 catcaatccg accggcgccg tgaattcgct gcaggaatgt ccccagccga attcgggatc
    19081 cgaaatattt ccctgataac agtaaggtgc tccgaaatcc tcacccactt tggtgacgcg
    19141 attaagttca tcctgaggaa catcttccga catccagtcg cgcccgttgt cggtgaagta
    19201 gagttgcttg gtttcaggat gccagtcgaa accgaccgag ttgcgaacgc caagtgccac
    19261 cacttcggcc ccggtgccat cgagattgat ccgcctgatc tggccgtgcg cgtcgtcatg
    19321 caagacgttg ttgccgggct ggccgaccgg aacgtacaat ttgttgtcag gcccgatacc
    19381 gatgaacttc cagccatggg cttcgtcctt gggcaggttg tcgtagatca tggttggttt
    19441 tggcggattg tcgatgacgt cctcggcctt ttcgatcttg gaaacttttg agagttcggc
    19501 aatgtagagc gtgccgttcc tgaacgccac gccgttgggc cgatagaggc cggaggccag
    19561 caccttgacc gatcgcttgc cgtccttgtt gacgacggca tagaccttgt cgacgaggcg
    19621 gctgccgacg aacaccgtac ccttgtcgcc ttcggccagt gaccgcgcat tcgccatgcc
    19681 ggcggcatag acctcgatgt tgaagccggc cgggacctta agcttggcgg tcggcagctt
    19741 gtccggagcc gctgcaatcg gaggcggcgc caccggcgca agttttgcgg cggcttcgct
    19801 ggccggccgg ccgatgagcg gtgaacccgg tggaagcgcc ggcgcggcag tcgccgctgg
    19861 agccggatcg gccggctgct gcgcgaccgc gccgaacgta aataaaccaa caaaggcgcc
    19921 agccagcaaa gcgctgcgcg gcgcggacga gcagatcatc atgatgtcct ccccttgagt
    19981 ggaagcctta tgtccttgcg gcacccgcgc gaagtggctt cctaaaccgg cactctccga
    20041 tatttttccg tcgatgcgca atcggaaaac atgcagggga gtcattccgg gatggtccca
    20101 acggggcggc gctttgcgct gacccgttgg cgtgcccggg aatgatgggg ggtcgcagaa
    20161 taattgcggc tcgcgtcagt gcttggtgaa gggcccgtcg atcatgtcga gcatcgcttc
    20221 ggcaaaggga aactccagca cgacctcgcc ttcatcatcc gtcacttcga ggatcgcact
    20281 caaaaggcca cgctcggcgc cttcggtcct caagagttcg cggatcatcg cccgcgcgac
    20341 ttcccatgca cgatcaagat cctgcagttc ctcgccttcc gggtcggaga tcaattcgtc
    20401 gccgatctgc gtgttgaaaa agtatcgcgg catggatcac gaaccacttc cgttaagggc
    20461 cccggcctga gccgggcgac atcacaaggt ctttatctca aacttcgccg tgcagcgccg
    20521 agaagtctgc attcaggtag atcgacggac attttgaagc tgttgatccg cctttgtttt
    20581 gacgccggcc cgcacaaata agtgaatttc tttcgcgttg cagcataaaa atagaggcgt
    20641 cggatgacaa aagatttcac tggcgaagtt ttcttatccg actagtttaa ctgaagggcg
    20701 gagttcgtcc catttacacg gagagccaaa atggcctgga aaacccctaa gatcgtcgaa
    20761 gtgccggttg gcatggaaat caacatgtat gcgtgcgccg cccgcaagta agcgggcgcc
    20821 acgacttcac gcttaacgca ggtggacctc ccggggtgac acgctcctga tcgggagcgt
    20881 gtttgtgtcg gagtccacct ttgcagtttt gaataacaag cctcgaatat taagaagcct
    20941 cgaatatcaa ggcttgggaa tctccaggag acggatcatg cttcgcgtcg tcgtcctggg
    21001 cgccgcggca ggtggtggcg ttccgcaatg gaattgcgga tgtccggttt gccggatggc
    21061 tcgaaccggg catcccgaac ttcagagcac acaggcttcg atcgcgatca gcgccgatgg
    21121 cgataactgg tttttgatca acgcgtcgcc cgatctgcgt cagcaactga tcgcgacgcc
    21181 gcagttgcac cccaaagccg gaaaacttcg ccacagtccg atatccggcg tcatcctgac
    21241 caatggcgaa atcgatgcgg tcgcgggact gttgtcgatg cgcgaaggct ggcccttcac
    21301 gatctacgcg cacccgcggg tgctttcgat cctgaacagc aacagcatct tcaacgtgct
    21361 gaacgagaag aacgtgccgc gccggccgat cgaggtgaac gagacgtttg agccgacctt
    21421 gccggatggt tcaccctccg gcatcgaaat cttgccgttc gctgttcccg gcaagagcgc
    21481 gtggtacctg gaaggcaagg atcaccccgc ggggggcgat ggcgcaggcg acacgctcgg
    21541 acttcgcgtc agcgacaggg caaccggcaa atgtctttat tttcttgctg cctgcgccca
    21601 tgtcacggac gatctcaagt cccgtctcgg cggcgcttcg ctgatattct tcgatggcac
    21661 ggtgtggcgc gatgacgagt tgatcgcagc cggcctcggc aacaagacag ggcaaggcat
    21721 gggacatatt gcaatgtcag gcaccaatgg cgcgatcgaa gccttggccg gactcgatat
    21781 tgaccggaag gtctttctgc atatcaacaa ttccaacccg gcgctgctgc acgactccgc
    21841 ggagcgcgag atcgccgagc gcgccggctg gcaaattccc gcggatggaa cggagatcac
    21901 gctttgaacg ccatcaccgc gaacaccatg acggccttct cgcgcggcgg cagcgcgccc
    21961 ttgaacagcg ccgaggaact ggaagcagcg cttcgtcaca tcggcgcgac gcgctatcat
    22021 aatctgcatc cgtttcaccg tctgctgcac ggcggcaaac tgaacaaggg acaggtgcag
    22081 gcctgggcgc tgaaccgcta ttattaccag agcaccattc cgatcaagga cgccgtggtg
    22141 atctcgcgct tccgcgaccg cggcatgcgt cttgaatggc ggcaccggat cgaggaccat
    22201 gacggcgatc tcggcaccga gggcggcatc gagcgctggc taaagctgac cgaaggcctg
    22261 gggctggaca gcgcttacgt ggaatccacc gagggcatcc tgccggcaac gcgctttgcc
    22321 gtggaagcct atgtgcattt cgtccgcgac aggaccccgc tcgaggccat tgcctcctcg
    22381 ctgaccgaac tgttcgcgcc caatctgcat gaagagcgta tttcgggaat gctggcgcat
    22441 tacgatttcg tcaaccccga tatcatgagc tatttcagtc gccgcatgca gcaggcgccg
    22501 cgcgatgcca acttcgcgct cgactacgtc aagacccatg cgaacacgcc cgaacagcgc
    22561 gaggcggtct gcaatgcgct gcttttcaag accaatgtgc tatgggttca gctcgacgcg
    22621 ctgtatcatg cctatgtcga cggccacgtg ccgccggggg cgtttgtgcc caagataaac
    22681 taagagataa actaagagat aagctaaaag acaaaaccaa ggccaaaccg gcaaccagag
    22741 gcgatgcaat ggcgggcctg cgcagccgca acatccgtgt cagcgaggcg agccggccgg
    22801 ttctgccgcg gcacgccaag ctcaaattcg atgagacgcg gcaagtgtgg gtcatcctgg
    22861 cgccggagcg cgtgctggcg ccggacaaga tcgcggtgga agtgcttcaa ctctgcgacg
    22921 gcgtccgcag cgtcgcggcg atgatcgatc tgcttgccga aaaatacacc gccgaccgtg
    22981 cggcgatctc aaccgacgtc gttgccatgc tgcaggacct tgccgacaag ggatttttga
    23041 ccgaagcgcg cgagaagacg tcatgagtcc gacgctcaca gatgccggcg tagccgccag
    23101 cgatccctcc gacgggctcg cggtgcttga ggcgcgcggc tcgaccgcgg aaaccttcgg
    23161 cattccgctc gccgtgctag ccgaattgac gcaccgctgt ccgttgcaat gtccttactg
    23221 ctccaacccg gttgagctcg agcgcggcag cagcgaattg accacggcgg agtggaagaa
    23281 ggtgctctcc gagctggccg aaatcggcgt gctgcagatc catttctccg gcggcgaacc
    23341 caccgcgcgc aaggatctgg tcgagcttgt ccagcacgcc agcgatgtcg ggctgtactc
    23401 caacctcatc acctccgccg tgctgctgac ccgcgaaaag cttgccgcac tggccgacgc
    23461 ggggctgtgc catatccaga tcagttttca gggcaacgag cctgtcgtcg ccgaccgcgt
    23521 cgccggtttg aaaaacgcac acgagaaaaa aatcgaagtc gcgaagtgga cgcgcgagct
    23581 ggatttgccg ctgacggtca acgcggtgat gcatcgccaa aacctgcatc agctttcgga
    23641 catcatccgg atggcggtcg atctggacgc cgaccggctg gaggtcgcca atgtgcagta
    23701 ttacggctgg gccctgaaga accgcgcggc gctgatgccg accctcgcac agatcgaaga
    23761 gaccagccgc atcgtcgagg aagccgaagc ccggctcaag ggcattctgg cgatcgacta
    23821 tgtggtgccg gattattacg cgctgcggcc gaaaaaatgc atgggcggct ggggccggca
    23881 gttcttcaac atttcacccg ccggcaagat cctgccctgt cacgccgcgg aaaccattac
    23941 cggactcgaa ttcctgtcag tgcggtcgaa tcattcgatc gcctggatct ggcagaattc
    24001 cgaggctttc aatcgctatc gcggcaccgg ctggatgccg gagccgtgca agagctgcga
    24061 gttcagggaa atcgatttcg gcggctgccg ctgccaggcc ttcgcactga ccggcgacgc
    24121 cgccaatacc gatccggcct gcacgctgtc gccgctgcat gagcagatct tcaagttggc
    24181 ggaacaggaa gccgctcgcg acaacaagcg tttcatctat cgcaattttg ccgggggtac
    24241 gctggagacg ggcggcgatc atggcgcctg acgccgacgc ggggaaagcc agcggcaacc
    24301 gttccgatcc gttcgcaccg ctgacatcgc agctgctgcc ggtcggcgac ggccacgaga
    24361 tctacgtcga agccgtcggc cgggccgacg gcatccccgc ggtctatctg cacggcgggc
    24421 ccggcagcgg ctgccagccc gatcaccggc ggctgttcga tcccgagcgt tttcacgccg
    24481 tcttgttcga tcagcgcggc gctggtcgta gccgcccaaa aggccaccgc gagcacaata
    24541 cgctgccgca tctgatcgcg gacatggaga tcatccgcga gaaatttggc tttgagcgct
    24601 ggatgatcgt cggcggctcc tggggtgcga cgctggcttt ggcctatgcg caggcccacc
    24661 ccgaccgggt caccggcatc gtgctgcgtg cgaccttcct cggcacccgc gccgagctgg
    24721 agggcgcatt tctcgacgcg ctgccgcgct tttatcccga tctcagcgat gattttctca
    24781 aattgttgcc accggaggaa cgcgcgcagc cgctcgatgc ctactggcgc cgcattctcg
    24841 attccgatcc tgcggtgcac ggtccggccg cccacgcgtg gggcgacacc gaacggatac
    24901 tttccggaca tgtttcgggg cgtgtccggc tcgattccca ttcgccaaac tcgacgcgcg
    24961 ccctgcccgc cacgccgttc atggaagcgc attactttca gaacaactgc ttcatgcggc
    25021 cggggcagct gctggcggaa gcaagcaagc tggcgggcag ccccgcgatc atcgtgcaag
    25081 gccgctacga tctgttgtgt ccgccgtcca tgtcgcatgc gctggcggcg gtgtgggccg
    25141 gttccgaagt ccgcgccgtc gagggcgccg gccatcagct gtacgatccc ggcgtccggg
    25201 atgccgtgat gaaggcgatc gcggatctcg cttccaaaat caccagatag ggacgtttat
    25261 aaaatgccgt tggccggtaa aggaatgctg ctgacatcga tggacatcga tccatcggac
    25321 gaagccgagt tcaatcgatg gtacgaccgc gagcatctgc tcgagcgcgt cgcgatcgac
    25381 ggctttcttg aagcccggcg ctacatcgcg catcagggct ccccgaaata tctctgcctc
    25441 tattccaccg cgactttcga ggtgctcgac agtcccacct atcgcaccgt tctgatgaac
    25501 cccaccgatt ggtcgaaggc aactctcgcg cgtttcaaga acatgattcg cgcgatcgca
    25561 cgcatcacgg tcagccgcgg tcagggccgc ggcgcggcgc tcggcatcgt ccgcctgcgt
    25621 ccccgtgccg gcgacaagga cgaattgcgc accgcactgc gggatcggct cgaccccgaa
    25681 aagctggacg gcatcatctc catgcacctg atcgagagcg atccggcact ttccaggccg
    25741 atcaccgacg atcccccggt ttccgatccg ggttccggcg actggttcgt gctgatcgat
    25801 gcgacggatg tgcgtgcggt atcggcggcc atcaccgacc gcctcgggga tgtgccaggg
    25861 cagatggccg ccgaaacgat ctcgaccggc gtctacaaac tgatgtggga cctggcgaag
    25921 agcgacatca ggtcgtccta gatcaaggca tgaacctcag aacatgatat ttgctgcagt
    25981 gccatatttg tccggtcggc attatgccat atttatccgg tcggcattaa agctggggtc
    26041 ccagactaat agcgaatggc gattaagcaa aaatggcttt tcagtggatc gtcgatcata
    26101 ttgaatggca ggattcatta agtccatgat atggcagcac atccataaca acgccgtgat
    26161 gggcgtttac aacagaaatc aaaaggcccg cgcacgctcg cactcacgcg gacatggtag
    26221 gaatgaaacg ctggaatgaa accgactgat atttcggctc ccgactactt tcataaagtt
    26281 gtcgattgcc aatgggcctg tcccgcgcat acgccagttc ccgaatacat ccggttgatt
    26341 gctgaaggcc gctacagcga cgcctacatg atcaattgga gatcgaacgt gtttccggga
    26401 attctcggac gcacctgcga tcgaccgtgc gagcccgcct gccgccgcgg ccgcgttgag
    26461 gagacgccgg tcgccatctg ccggctgaag cgcgtcgccg ccgatttcaa ggacgacgtc
    26521 aaacaccgca tgccacggcc ctcgccgaaa aacggcaagc gtatcgcgat cgtcggcggc
    26581 ggccccgcct cgctgaccgt ggcccgcgat ctcgcgccgc tcggctacca ctgcacggtg
    26641 ttcgattccg acccgaaggc cggcggcatg atgcggacgc agattccgaa attccggctg
    26701 ccggattcgg tgatcgacga ggaaaccgat tacatcctca atctcggcat cgagttcaag
    26761 ggcggcacgc gcatcgacag cctgaagaag ctgcttggcg aaaagtttga tgcgatcttt
    26821 gtcggttccg gcgcgccgcg cggccgcgag ctcgagattc ccggccgcaa ggaagcagcc
    26881 agccacatcc atatcggcat cgactggctc tccagcgtgt cgtttggcca tgtcgaaaag
    26941 atcggcaagc gcgtgatcgt gctcggcggc ggcaacaccg cgatggactg ctgccgcacc
    27001 gcgcgccgtc tcggcggcga ggacgtcaag gtgatcgtgc gttccggttt cgaggagatg
    27061 aaggcgagcc cctgggaaaa ggaggacgcc ctgcacgagg acattccgat cctcaatttc
    27121 atggtgccga aggcgttcac ccacaacaat ggcaagctca ccggcgtcac cttccagaag
    27181 gtgaaggccg aatatgacga caaggggcgg cgcaatctgg tgcccgcggg cgacccggac
    27241 cagaccatcg cctgcgacga cgtgctggtg gcggtcggcc aggaaaacgc ctttccctgg
    27301 atcgagcgcg actgcggtgt cgaattcgac aagtggaaca tgcccaaggt cgacgccaag
    27361 accatgggct cgaccaatcc aaaagtgttc tttggcggcg acgccgcgtt cggccccaaa
    27421 aacatcatct gggccgtggc gcatggccac gatgccgcgc tttcgatcca caagctgctg
    27481 tccggcgagg acattgccga gcgcccgctt ccggacgtga agatctcatc gcagaagatg
    27541 ggcatccacg aatggagcta cgacaacgac gtctccgccg acaagcgctt caaggtgccg
    27601 catcgcgaca agatcatcgc gctcaaggac atccggtcgg aagtcgaact cggctacgac
    27661 gtcaagctcg cgctcggtga ggcacagcgc tgcctgaatt gcgacatcca aaccgtgttc
    27721 gcggccccac tgtgcatcga gtgcgatgcc tgcgtcgata tctgcccgat ggactgcatc
    27781 tcctttacgg agaatggcga ggaggccgat ctgcgcacgc ggctgaaagc gccgtcgccg
    27841 catctcgacc aggaccttta tgtcgccgac ggcctcaaga ccggccgggt gatggtgaag
    27901 gacgaagacg tctgcttgca ttgcggcctg tgcgccgaac gctgtcccac cggcgcctgg
    27961 gacatgcaga aatatctcat cgacatgact cacgcgggtt catcatgcca gtcaaagagc
    28021 cgatcagcag cgtaaacgac ttcgtcgttc gcttcgccaa cgtcaacggt tcgggatcgg
    28081 caagcgccaa cgaactgttc gcccgctcga tcctgcgcca cggcgtgccg gtttcgccgc
    28141 gcaacgtctt tccctccaac atccagggac tgccgacctg gtacgaggtg cgggtcaccg
    28201 aggccgggca cctcggcgcc cgcggcggcg gcgtcgacat gatggtggcg atgaacccgc
    28261 agacctggga caaggacctc gcctcgatcg agcccggcgg ctatctgttc tacgattcga
    28321 ccaagccgat gccgacgtcg aaattccgca ccgacgtcac ggtgatcggc gtgccgctga
    28381 cggccatcac caattcgacc tataccgacc cgcgccagcg ccagctgttc aagaacatca
    28441 tctatctcgg cgcgatgtgc gccctgctcg acatggatcc gaagctcgtc gagcaactga
    28501 tcggcgaaca atacaagggc aaggaaaagc tcttgtcctc gaacatccac gcgctgcatc
    28561 tcggtcgcga ctgggcgctg caaaatctga aatgtccaat cggcctgcgc gtcaaaaagt
    28621 ccgacaaggt cggcgaccgc attttcatcg agggcaatag tgcggccgcg ctcggcgcgg
    28681 tatatggcgg cgccaccgtg tgcgcatggt acccgatcac accttcatcg tcgctcgccg
    28741 acgccttcgg cagtcactgc aagaagctgc ggcacgatcc taagaccggc aaggcgaaat
    28801 atgccatcgt tcagggcgag gacgaactgg cttcgatcgg catcgtgatc ggcgcgtcat
    28861 ggaatggcgc gcgcgccttc actgccacct ccggtcccgg catctcgctg atgcaggagt
    28921 tcatcggcct gtcgtacttc gccgaaattc cggccgtgat catgaacgtg cagcgcgccg
    28981 gtccctcgac cggcatgccg acgcgcaccc agcaatgcga catcctggcc tgcgcctatg
    29041 cctcgcacgg cgacaccaag catgtgctgc tgtttccgga agatccggct gaggcgtttg
    29101 aatttgccgc gcaagccttc gatctcgccg aacgcctgca gaccacgatc ttcgtgatgc
    29161 tcgatctcga catcggcatg aaccacaggc ttagccgtcc gctcaagtgg gacgacgcca
    29221 ggcaatacga ccgcggcaag gtgatgacgg ccgagatgct ggacgatggc cgcgacttcg
    29281 ggcgctatct cgacatcgac ggcgatggca tcgcctaccg cacctatccc ggcacccatc
    29341 cgaccaaggg ctcgttcttc acccgcggca cctcgcgcga ccgttacgcg cgctattccg
    29401 aagagggtgc ggtttacgcc gacaacatgc agcgcctggt gcgcaaattc gagggcgcgc
    29461 aggatatggt gccgcggccg ctgcaggcca acgccgccaa gctcaccaaa tacggcgtga
    29521 tctatttcgg ctcgactgcg ccggccatgg acgaggcgat cgggctattg gaggcgcgcg
    29581 ggcatctgct cgacaggctt cggatccgcg ccttcccgtt ccattccagc gtcgcaagct
    29641 tcatcgccga ccacgacttc gtctacgtgg ttgagcagaa ccgtgatgcg cagcttcgtt
    29701 cgctgatcgt taacgagaac ggcatagatc cggttcggct gatcccgatc gtgcattatg
    29761 acggcacacc gattaccgcg cgcttcatcg ccggcgccat cggcggccat caggaccagc
    29821 tcaaactaac cccgctccgc aaggtgaagt catgacctat atcgtcaagc cgaagttcca
    29881 gcatcccggc cttccgaaga acgatctcgg ctttacccac cgcgattacg agggcaagat
    29941 ttccacgctg tgcgccggct gcggccacga ctcgattacc gcatcgatca tcgaggcctg
    30001 ctttgagctc tcgatcgaac cgcatcgcgt cgccaagatt tccggcatcg gctgctcctc
    30061 gaagacgccg gactatttcc tcggcagctc gcacggcttc aactcggtgc atggccgcat
    30121 gccgtcggtg ctgaccggcg ccaacctcgc caaccgcgac ctgatctatc tcggcgtttc
    30181 cggcgacggt gactcggcct cgatcggctt cggccagttc gcgcactcga tccggcgcgg
    30241 cgtcaacatg acctatatcg tcgagaacaa cggcgtctac ggcctcacca agggccagtt
    30301 ctcggccacc gccgaccgcg gctcgaagaa caagcgcggc gtgatgaata ccgacaacgc
    30361 catcgacctg gtggcgatcg cgcttcagct cggcgcgagc ttcgtggcgc gcagcttctc
    30421 cggcgacaag acccagctgg tgccgctgat tgcggctgcg atccgccaca agggcgcgtc
    30481 gttcatcgac gtcatcagcc cctgcgtcgc cttcaacaac catgccggct cgaccaaaag
    30541 ctttgattat gtgcgcgagc acaatgatgc ggtgaaccgg ctcgacgtca tcaccggccg
    30601 cgcgccgatc aaggtcgatt atgctcccgg ctccgtccaa attgtcgagc agcacgacgg
    30661 atcgaaactg gtcttgcgca agctcgacag cgactacgac gtccatgatc gggtcgccgc
    30721 catgggcttt ctgcagaccc acgccgccaa gggtcaaatc gtgaccggcc tgctctacgt
    30781 cgacggggaa cccgaagacc tgcaccatca tctcaacacc gtggacacgg cgctcaacgc
    30841 actctctgag aaagagct
//
LOCUS       DATNOI010000423         2801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1630275,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000423 DATNOI010000000
VERSION     DATNOI010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630275"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..841
                     /locus_tag="VN968_17025"
     CDS             170..841
                     /locus_tag="VN968_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HXL30971.1"
                     /translation="MLWAALIIVTAVFLLPYLLVPLYRVVNPVSTLMLWRWANGARVE
                     RTFVPVERMAPALPVTVIAAEDGRFCRHRGIDWQEIRDRLEDVDDINEAKGVSTITQQ
                     TAKNLFLWPGRSMARKALELPLALWIDLALPKWRVLEIYLNIAEWGPNGQFGAEAASR
                     YAFNKSVRMLTAREAALLASVLPNPRRRSAKQPGPAVRRLAAIYEARGAAQAALAACA
                     KPLRP"
     gene            complement(944..2098)
                     /locus_tag="VN968_17030"
     CDS             complement(944..2098)
                     /locus_tag="VN968_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HXL30972.1"
                     /translation="MEDLSAFSAKPRLRLLLDHFAKIKDTRQAWKVAYPLCEVLFLVV
                     CGTIANGDDYEDIVDWGKAHLSFLRGFAEFHYGIPCADWLRTVMNRINPDLFQACFSS
                     WVAECWPDKLDLVAIDGKTSRRSHNRKTGQKPLHLVSAFATNSRLVLGQEAVDEKSNE
                     ITAIPALVKRLDLEGALVSIDAMGCNPNIAQSILDAKADYLLAVKDNQPTLHAEIKSY
                     FQTAPSLEVEQIETVGKDHGRIEVRTHTVSHVVDWYTAQRSYPGAPRFPQLTTIAMVE
                     SRIERGDKIETEQRSYISSRPLSAAAFADGARGHWGIENNLHWTLDVTFNEDQSRLRV
                     GHGANNMAVVRHFALNLVRQIADKRSIKRRRKCAAWDPKYLLEILGPLRR"
     gene            2343..>2801
                     /locus_tag="VN968_17035"
     CDS             2343..>2801
                     /locus_tag="VN968_17035"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HXL30973.1"
                     /translation="MRKSNKEAAQTREAIVTAAADHIRRTGIAEASLADVMAAAGLTH
                     GGFYRHFRNKEHLVAEAFSAAGDKTIATISRTMSKGGVNAVVDSYLSALHRDAPTPIC
                     PLAALGSEVARSGGETKAAATEVLEKLLVTLADSRSGPEARGDAIVALATM"
BASE COUNT          534 a          855 c          881 g          531 t
ORIGIN      
        1 gcgtccttgg ccatgaagat gcctcttcgc cctttgcttc gccttgcttt accagtgcga
       61 gccaatctct gctacagcct caagacaagg tggaatggta ccccctgcaa cctcacggcg
      121 gcctggtctc gcgaaacagt cgccgcgccc gcacccttgg ctcgcgcgct tgctgtgggc
      181 cgcgctgatc atcgtcacgg cagtcttcct actgccgtac ctgctggtgc cgctctatcg
      241 cgtggtcaat ccggtttcga cgcttatgct ctggcgctgg gcgaacggag cgcgtgtcga
      301 gaggacattc gtgcccgtcg agcgcatggc gccggcgttg ccggtcaccg taattgcggc
      361 cgaggatggc cgtttttgcc ggcatcgcgg gatcgactgg caggaaatcc gcgatcggct
      421 cgaggatgtg gacgatatca acgaggcgaa gggagtatcg accatcaccc agcagacggc
      481 caaaaacctg ttcctgtggc cgggtcgaag catggcacgc aaggcattgg agctcccgct
      541 ggcgttgtgg atcgatctgg ccttgccgaa atggcgtgtg ctcgaaatct atctcaacat
      601 tgcggaatgg gggccgaatg gccaattcgg cgcagaagcc gcgagcaggt acgccttcaa
      661 caaatcggtg cgcatgctga ccgcgcgcga ggcggctttg ctggcctcgg tgctgcctaa
      721 tccgcggcga cgcagcgcca agcaacccgg accggcggtg cgcaggcttg ccgctatcta
      781 tgaggcgcgc ggcgctgcac aggcagcgct tgcagcttgt gccaagccgc tccgcccgtg
      841 acgccgccgt catcgagatg aaacgacgcc tgggtctacc ccaacgggcc gacgcggcgg
      901 ccctcgctcg acccacgcgc gaccccaggg caacgagtcg aggttaacgc cgcagcggcc
      961 ccaatatctc cagcaagtat ttgggatccc atgcggcgca tttgcggcgg cgcttgatgg
     1021 atcgtttgtc ggcgatttgg cgcaccaggt tgagggcgaa gtggcggacc acggccatgt
     1081 tgttggcccc gtgaccgacg cgcaggcgtg actgatcctc gttgaaggtc acgtcgagcg
     1141 tccaatgaag gttgttttcg atcccccaat ggccgcgtgc gccgtcagcg aaggctgcgg
     1201 ccgagagcgg ccgggaggag atataggatc gctgttcggt ctcgatcttg tcgccgcgct
     1261 cgatgcggct ctcgaccatg gcgatggtgg tgagttgggg gaaacggggt gcgcccggat
     1321 aggagcgctg cgcggtgtac cagtcgacga catgggagac cgtatgggtg cgaacctcaa
     1381 tgcggccgtg gtccttgcca accgtctcga tctgctcgac ctcaagggac ggcgctgttt
     1441 ggaaatagct cttgatctcg gcatgcaagg ttggctggtt gtccttcacg gctaacaggt
     1501 agtcggcttt ggcgtcgagg atcgactggg cgatattggg attgcatccc atagcatcga
     1561 ttgagaccag ggcaccctca agatcaaggc gcttcacaag ggccggaatg gcggtgatct
     1621 cgttcgattt ttcatcgacc gcctcctggc ccagcaccag ccggctgttg gtggcgaagg
     1681 ccgacaccag atgcaggggc ttctggccgg tctttcgatt gtggctgcga cgcgaagtct
     1741 tgccgtcgat cgccacgaga tcaagcttgt ccggccagca ttccgcaacc caggaagaaa
     1801 agcaggcctg gaacaggtcc gggttgatgc gattcataac cgtgcgcagc caatcagcac
     1861 agggaatgcc gtagtggaac tcggcaaagc cccgcagaaa cgacaaatgc gccttgcccc
     1921 aatcgacgat gtcctcatag tcgtcgccgt tcgcgatcgt cccgcacacc accaggaaca
     1981 gaacctcgca cagcggatag gcgaccttcc aggcttggcg cgtgtctttg atcttcgcaa
     2041 agtgatcgag cagcaggcgc agccgtggct tggctgaaaa ggccgataag tcctccatcg
     2101 aaccctcccg aatcggtagg gtcccagaga atcagtccgt cacgccgccc gctagaacct
     2161 ttcgctccgt caatcagaaa aacgcgttaa cctgacctcg gcgccctggc gggtatctga
     2221 catctcattg actccacgtt actttcatct aaattagatg tcgtatgaca tcgaattaga
     2281 tgtcaggtat catccaatct gtcaagcccc tcatgcagag tgggcccaga agggaccagc
     2341 cgttgagaaa gtcgaacaaa gaagcggcgc agacgcgaga agcgatcgtc accgccgccg
     2401 cggaccacat ccggcgaacg gggatcgcgg aggcgagcct tgccgacgtg atggcagcgg
     2461 cgggcctcac ccacggtggg ttctatcggc atttccgcaa caaggagcac ctcgtcgccg
     2521 aagcgttctc cgctgctggc gacaagacga tcgcgaccat tagccggacc atgtcgaagg
     2581 gcggcgtcaa cgccgtggtc gacagctatc tgtcggcctt acaccgcgac gcgccgacgc
     2641 cgatctgtcc cctcgcggcc ctgggtagcg aggtggctcg atctggtggt gagaccaagg
     2701 ccgcggcgac ggaggttctg gaaaagctgc tcgtcactct cgctgacagt cggtccggac
     2761 ccgaggcacg aggtgatgcg atcgtcgccc tcgccacgat g
//
LOCUS       DATNOI010000424        14700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2066623,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000424 DATNOI010000000
VERSION     DATNOI010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 14700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14700
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066623"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VN968_17040"
     CDS             complement(<1..422)
                     /locus_tag="VN968_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXL30974.1"
                     /translation="MAAETRHATQEITAIRRADGESRTLAASLPRLVLEARRIAANVI
                     HGLHGRRRAGAGESFWQYRRFVSGEPSQSVDWRRSARDDHLYVREQEWEAAHTVWLWP
                     DRSPSMAFASKQARDSKLERGLIVTFALAELLVAGGERV"
     gene            complement(422..1426)
                     /locus_tag="VN968_17045"
     CDS             complement(422..1426)
                     /locus_tag="VN968_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXL30975.1"
                     /translation="MAGADSVEKLEDVIVRSAEQVAGQIRAAKDAISTVIFGQDRVIE
                     NTLVTILSGGHALLIGVPGLAKTKLVETLGITLGLDAKRIQFTPDLMPSDILGAEVLD
                     ESTSGKRSFRFISGPVFAQLLMADEINRASPRTQSALLQAMQEQHITVAGARHDLPKP
                     FHVLATQNPLEQEGTYPLPEAQLDRFLMEIDVDYPDRDAERRILFETTGADETLAKAA
                     MNADILITAQRLVRRLPVGDSVVEAILTLVRSARPGEEGGEAGKLIAWGPGPRASQSL
                     MLAVRARALLDGRLAPSIDDVLDLAEPVLKHRMALTFSARAEGRTIPDVIRQLKTRIG
                     "
     gene            1684..2292
                     /locus_tag="VN968_17050"
     CDS             1684..2292
                     /locus_tag="VN968_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HXL30976.1"
                     /translation="MAKQGQSKHQGLEGLTAAVKDVANASAARGLPPVHLWNPPFCGD
                     LDMRIGSDGTWFYMGTPIGRPALVRLFSTILKREDGKHFLVTPVEKVGIRVDDAPFLA
                     VEMLKETDRRGRVLRFRTNVDDWVPCDSAHRLRFEAAADGGLTPYLHVRADLWAKVTR
                     ALYYDLVDMGEERVVDGRPMFGIDSAGEFFSMADAEQVRGAL"
     gene            2289..2960
                     /locus_tag="VN968_17055"
     CDS             2289..2960
                     /locus_tag="VN968_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HXL30977.1"
                     /translation="MNEPVLRMDPAAIGSAEFFDRARARLRFDIPAGLTDASVVPPSG
                     DQGTDRMLQIVAREQPIRPAAVLIPVVEHAQPTVLLTQRAAHLNDHAGQISFPGGKID
                     ATDASPLDAALREAEEEIGLSRQFIDPIGYLDLYATGFGFRILPTVARVKPGFRLRIN
                     QAEVDDAFEVPLAFLMNPANHQVHSKEFRGMERSYYAMPFEERYIWGATAGILRVLYE
                     RICLP"
     gene            2957..3220
                     /locus_tag="VN968_17060"
     CDS             2957..3220
                     /locus_tag="VN968_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HXL30978.1"
                     /translation="MIRPAFTEIGIFLIPFAVYALFLIATRSGLLVQSSWPIHIIGRL
                     VLGALVLVVISFILLAHFSGAPPNSTYIPAHIENGKFVPGVEK"
     gene            3217..4473
                     /locus_tag="VN968_17065"
     CDS             3217..4473
                     /locus_tag="VN968_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HXL30979.1"
                     /translation="MSDARVLDDAPWLKSGPTARLLGLLNGDGEEARVVGGAVRNVLL
                     QIPPGDIDIATTALPDEVIRRARAAGIKSVPTGIEHGTVTLVVDGHPFEVTTLREDTE
                     TFGRKAKVAFGRDWVRDAERRDFTINGLSVDAAGVVHDHVGGLDDILAKRVRFIGDPD
                     RRIAEDYLRILRFFRIHAAYGVGEPDRDGYLACIRLRAGLATLSAERVRMEMLKLVIA
                     DGAAGAVAAMADGGLLLPILGGIGYTGPFAAMISAERLLGLKPDAVRRLGALAVAVTE
                     DARRVAARLRLSNAETKALDSMGHRWWRLGGMDEAIARRRLYRLGEDRYRDRLMLAWA
                     RAGAGADSAHWRELAALPERWRAPKFPLKAADFVPRGITEGPALGRVLALAEDAWLAA
                     DFPLDGPALKAIADQTVTRFTRDHRP"
     gene            4470..5237
                     /locus_tag="VN968_17070"
     CDS             4470..5237
                     /locus_tag="VN968_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HXL30980.1"
                     /translation="MNILSGFSDISIAQLLLVTCVALFASVVGGLAGYGTGALMPLVL
                     VPLVGAEPVVPIIAISAIFTNSSRFAAYLRYADFRRAGIAAAAAAVTTAFGAYGYTRL
                     SNAGAALVIGAMLISSVPLRRLLKRRDVKIGDTGLAAGAVGYGVLVGGTSGSGVILLS
                     LLMAAGLEGAAVIATDAVISVTTSMIKISVFGLSGVVTAQVLALAVLIGAIALPGAFL
                     AKVFVERMPVHIHTAILDVAVIIGGVVMISAAARRFW"
     gene            complement(5352..5561)
                     /locus_tag="VN968_17075"
     CDS             complement(5352..5561)
                     /locus_tag="VN968_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HXL30981.1"
                     /translation="MGLKPGDVVVLKSGGHPMTVAEVDDDDIVCVWMGTEGELFRETL
                     SLAVLEAAEIDDDEEEDDDEEEEEE"
     gene            complement(5769..6656)
                     /gene="hemF"
                     /locus_tag="VN968_17080"
     CDS             complement(5769..6656)
                     /gene="hemF"
                     /locus_tag="VN968_17080"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HXL30982.1"
                     /translation="MNTDDIEDRKARARSWFESLRDDICQAFERLEDDAPASLYPGSA
                     GRFVRTPWDRTDHTGKPGGGGLMSVMRGRLFEKVGVHCSTVHGEFSPEFRSQVQGASE
                     DPNFWASGISVIAHLRNPHVPAVHMNTRFVVTTKAWFGGGSDLTPVLDRRRTQQDADT
                     IAFHTAMQAACGGHNGVADYDKYKKWCDDYFYLPHRKEARGIGGIFYDHHDSGDWNAD
                     LAFTQEVGRAFLKIYPELVRRNFDAPWTAQDREEQLIRRGRYVEFNLLYDRGTIFGLK
                     TGGNVDSILSSMPPEVKWP"
     gene            complement(6705..7070)
                     /locus_tag="VN968_17085"
     CDS             complement(6705..7070)
                     /locus_tag="VN968_17085"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HXL30983.1"
                     /translation="MWELTKSFRFEAAHALGGTTLGAASEEIHGHSFRAEVTVRGTPD
                     PATGMVLDLGLLERSMTEVRKMLDHKLLNKVDALGPPTLENLSRFIWDRVQRAGPITR
                     VSVHRDSCNESCAYYGPQG"
     gene            complement(7087..7719)
                     /gene="queE"
                     /locus_tag="VN968_17090"
     CDS             complement(7087..7719)
                     /gene="queE"
                     /locus_tag="VN968_17090"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HXL30984.1"
                     /translation="MSYAVKEIFLTLQGEGAHAGRAAVFCRFSGCNLWSGREQDRATA
                     TCRFCDTDFVGTNGTRGGRYATAGELADTVAAQWTGIGANRYVVLTGGEPLLQVDEAL
                     VDALHTRGFEIGIETNGTIEPPEGMDWICVSPKAGADLVIRRGHELKLVYPQADAVPE
                     AFVDLAFERFSLQPMDGPDVIENTARTIDYCLRHPQWRLSLQTHKTLGIR"
     gene            complement(7902..10577)
                     /locus_tag="VN968_17095"
     CDS             complement(7902..10577)
                     /locus_tag="VN968_17095"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXL30985.1"
                     /translation="MDHENSTGAAHMQISVLLDRSRPDSLTTQMVDQIRDAIRCARIG
                     PGTRMPSSRRLSEQLAISRNTVMRAYDLLLMEGIVESRPASGIYVAEPLPLNSAAMQP
                     ASDPRDHIMQVRMPMPLRQGRTQGAAHAARNRLLYDFFPGHPGADLFPLKTWRRLLQA
                     NLSHGGAVGLTQYGEPAGLPALRTAIANHLVAARGIVADPSRIVIISGIQEGLTLLAR
                     LFLARGSLSVVEDPCYRGAALTFEAAGAEIASVAVDQDGLIPDYLPQRAASLLYATPS
                     HQYPTGATLSAERRREVIDWARRYGCYIIEDDYDCDIRYQGSHLPPLAALAPDCAVYM
                     GTFSKSLGAGLRLGYMVAPEHIAEAVRSEKCLLNNGNSWLEQATLADFMHNGSYGAHL
                     LRVRSHYRDNRDCLIAALRRNFGDVLVDGDSGGLHVLWHLPPGIPDAVTVEALALRAR
                     IGVYSLSSARVHFRRQTALTSRAIILGYAALSPKQIEKGIARLSDAVDDAIDDPRTDM
                     TALFSDQFASLLPPSPAKRHLAPRHRQRPALRSPSPYRAISGKIIARQGGAPMPVLKN
                     IYRYPIKGLSAQPLVRVELEARKPFPHDRVFALVRPGAPFDINDPRWGKKGLFVMLML
                     EEQLARVRTTLDVETLQLTITQDNRQLIVADLNDDEARAKVADIFWQLVPALRSAPTL
                     VRSPDGHFMDKPDNVISLINLATVRSLEEHWGIKIDPLRFRANLYIDGARPWEEFEWV
                     GSDIRIGDGLFRVDRRNGRCGATNVNPETGRRDLDLPGSLRAAFGHKELGVYLIAREG
                     GRLAVGDQVFTPSTTAVGHFSLPLVGKPHIRHQKFMCGGCYFIYEPAAGSPDQAIPGG
                     TPFAAIPPSWRCPDCGTEKTTFRPHIEPQPSPTRI"
     gene            10954..12240
                     /gene="urtA"
                     /locus_tag="VN968_17100"
     CDS             10954..12240
                     /gene="urtA"
                     /locus_tag="VN968_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HXL30986.1"
                     /translation="MSTVRHRWLSAAAAVLAILGTGVNQAKAEDAIKIGILHSLSGTM
                     AISETILKDLMLMEVADVNAKGGLLGKKIEPVVVDPASNWPLFAEKARELLVKDKVAA
                     VFGCWTSVSRKSVLPVFEELNGLLFYPLEYEGEEASYNIFYGSSVPDNKAVPAVEYLM
                     SKEGGEVKRFVLEGTDYVYPRTSNKIIRAYLKTKGVKDEDIMENYTPFGFSDWQTEVA
                     AIKKFGSAGKKTAVVSTVNGDANVPFYKELGNQGVRATDIPVIAFSVGEEELAGVDTK
                     PLVGHLAAWSYFQSVNTPENKAFIAKWRAYAKNPRRVTNDPMESAYILFHMWVQAVQQ
                     AGTTDVEAVRQAMIGQRVKSPSGFEVVMGSNHHMAKPVMIGEIQADGQFSIVHQSKAL
                     PPKAWSPYVEANKGKVADWSWPWVCGGCTSPRFAAN"
     gene            complement(12359..12814)
                     /locus_tag="VN968_17105"
     CDS             complement(12359..12814)
                     /locus_tag="VN968_17105"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HXL30987.1"
                     /translation="MQIALFQPDIPQNTGTILRLCACLDVAAHIIEPAGFPVSDRHFR
                     RAGMDYLDQVTITRHDSWPKFEQWRSDGGYRLILFTTKGIDSYLDHRYGAADILLFGR
                     ESAGVPDEVVAAADARLVIPIKPGLRSLNIAMAAAMALGEALRQTREGA"
     gene            13107..13634
                     /gene="petA"
                     /locus_tag="VN968_17110"
                     /gene_synonym="fbcF"
     CDS             13107..13634
                     /gene="petA"
                     /locus_tag="VN968_17110"
                     /gene_synonym="fbcF"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HXL30988.1"
                     /translation="MTTASSMDSTRRDFLFVATGAAAAVGGLATLWPFVSQMNPDAST
                     IAAGAPIEVDLAPIAEGQDIKVFWRGKPIYISHRTKKQIDEARAVPVASLPDPQADQA
                     RVKEGHDQWLVVIGICTHLGCIPIAHEGNYDGFFCPCHGSQYDSSGRVRQGPAPANLP
                     VPPYQFVSDSKIQIG"
     gene            13698..>14700
                     /locus_tag="VN968_17115"
     CDS             13698..>14700
                     /locus_tag="VN968_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HXL30989.1"
                     /translation="MSGPSDFQPSHPALKWIERRLPIMGLVHSSFVAYPTPRNLNYWW
                     TFGAILSMMLGLQILTGVILAMHYTPEATMAFKSVESIVRDVNYGWLLRNMHASGASM
                     FFLAVYIHMFRGLYYGSYKEPREILWILGVIIYLLMMATGFMGYVLPWGQMSFWGATV
                     ITNLFSAVPYVGDSIVTLLWGGYSVGNPTLNRFFSLHYLLPFVIAGVVVLHVWALHVA
                     GQNNPAGVEAKTEKDTVPFTPYATIKDAFGISCFMLFFAWFIFYMPNYLGDADNYIPA
                     NPAVTPQHIVPEWYYLPFYAILRSIPNKLAGVVCMFGAIIILAFLPWLDNAKTRSSKY
                     "
BASE COUNT         2614 a         4605 c         4639 g         2842 t
ORIGIN      
        1 acccgttcgc cgcccgcgac caaaagctcc gcgagcgcga aggttacgat cagtccgcgt
       61 tccagcttgc tgtcgcgcgc ctgtttcgag gcgaacgcca tcgacggcga gcggtcgggc
      121 cacagccaca cggtgtgggc ggcttcccat tcctgttcgc ggacgtagag atgatcgtcg
      181 cgggccgagc gccgccagtc gacgctctgc gacggctcgc ccgaaacgaa gcggcggtat
      241 tgccagaaac tttctccggc gccggcgcgc cgccgtccgt gcaggccgtg aatgacgttg
      301 gcggcgatgc ggcgagcttc aagcaccagg cgcggcagcg atgcggcgag cgttcggctt
      361 tcgccatcgg cgcgtcggat tgcggtgatc tcctgggttg cgtgcctggt ctctgcggcc
      421 atcaaccaat ccgcgtcttc aattgccgga tcacgtccgg aatggtgcgg ccctcggcgc
      481 gggcggagaa tgtcagcgcc atgcggtgct tgagcaccgg ttcggccaga tcgagcacat
      541 cgtcgatcga cggcgccaga cggccgtcga gcaaggcccg tgcgcgcacc gcgagcatca
      601 gcgactggct ggcgcgcgga cccggtcccc aggcgatcag cttgccggcc tcgccgcctt
      661 cttcacccgg acgcgcggac cgcacaaggg tcaggatcgc ttcgaccacg gagtcgccga
      721 ccggcagccg ccgcaccaag cgttgcgcgg tgatgagaat gtcggcgttc atggcggcct
      781 tggccagcgt ctcgtcggcg ccggtggttt cgaacaggat gcgccgctcg gcgtcgcggt
      841 cgggatagtc gacgtcgatc tccatcagaa agcgatcgag ctgcgcctcc ggcaacgggt
      901 aggtgccttc ctgctccagt gggttttggg tcgcgagcac atgaaacggc tttggcagat
      961 cgtggcgcgc gccggccacc gtgatatgct gctcctgcat cgcctgcagc agcgcggact
     1021 gggtgcgcgg gctggcccgg ttgatttcgt cggccatcaa cagctgcgcg aacaccggtc
     1081 cggaaatgaa acggaacgac cgcttgccgg aagtgctctc gtccagcacc tcggcaccga
     1141 gaatatccga gggcatcagg tccggcgtga actggatgcg cttggcatcg agcccaaggg
     1201 taatccctaa ggtttcgacc agcttggtct tggcgaggcc gggcacgccg atcagaagcg
     1261 cgtgaccgcc tgacagaatc gtgaccaggg tattttcaat cacccggtcc tgaccgaaga
     1321 taactgtgga aatcgcatcc ttcgcggcgc ggatctggcc ggcgacctgc tcggccgagc
     1381 ggacaatcac gtcctcaagt ttttccacac tgtctgcgcc agccattggt cactccttgc
     1441 ctcaaaatgt cgccggttgc gacgtgatgt tatgaacccc ttgcacaacc gatgcgaagg
     1501 ccatgtattc gttgaattaa actatcccca cattatcggc atttcggggt atgaactgca
     1561 tcacgatgtg gcgaccttac cttaggatcc gttccgggaa ctcgtccaag acactcaccg
     1621 gatgacttgc agaagtcttg caccaatcgt gccaaaacga ggccatcaaa gtcagggcaa
     1681 accatggcga agcaagggca gagcaagcat caaggcctcg aaggactgac tgctgccgtc
     1741 aaagacgttg caaacgccag cgctgcaagg gggctgcccc ccgtgcattt gtggaacccg
     1801 ccgttctgcg gcgatctcga tatgcgaatc gggagtgacg gtacgtggtt ttatatgggg
     1861 acgccaatcg ggcggccggc tttggtccgg ctgttttcga caatcctcaa gcgcgaggac
     1921 ggcaagcact ttctcgtgac accggtggag aaggtcggca tccgcgtcga cgatgcgccg
     1981 tttctcgccg tcgagatgct caaggaaacc gatcgtcggg gccgagtgct gcgcttccgc
     2041 accaatgtcg acgactgggt accatgcgat tccgcccacc ggctgcgctt cgaggccgcg
     2101 gccgacggcg gcctcacgcc ctatcttcac gtccgcgccg acctgtgggc caaggttacg
     2161 cgggcgcttt actacgatct ggttgacatg ggcgaggagc gggtggtcga tggtcgtcca
     2221 atgttcggga tcgattcagc cggcgaattc ttttcgatgg cggatgcgga gcaggtgagg
     2281 ggcgcgcttt gaatgagccg gtgctgagga tggaccccgc tgcgatcggc tcggcggagt
     2341 ttttcgatcg ggcccgcgcg cggctgcgct tcgacatccc cgcaggattg accgatgcca
     2401 gcgtggtgcc gccgagcggc gatcagggca ccgatcgcat gctgcagatc gtcgcccgcg
     2461 aacagccgat ccgtcccgcg gcggtgttga tcccggtggt ggagcatgcg caaccgaccg
     2521 tgctgttgac gcagcgcgcg gcgcatctca acgatcacgc cggccagatc tcgttccccg
     2581 gcggcaagat cgacgcgacc gacgcttcgc cgctcgatgc ggcattacgc gaggccgagg
     2641 aggaaatcgg cctttcgcgg caattcatcg atccgatcgg ctatctcgat ctgtacgcaa
     2701 ccgggttcgg gtttcgcatc ctgccgacgg tcgcgcgggt gaaaccgggc ttcaggctac
     2761 gcatcaacca ggccgaagtg gacgatgcgt tcgaggtacc gctggcgttc ctgatgaatc
     2821 ctgcgaacca tcaggtgcac agcaaggaat tccgcggcat ggagcgctcc tactacgcca
     2881 tgccgttcga ggagcgttat atctggggcg cgaccgcggg gattctgcgt gtgctgtatg
     2941 agcggatctg cctgccatga ttcgtccggc cttcaccgaa atcggaattt tcctgatccc
     3001 gttcgcggtg tatgcgctgt ttttgatcgc gacgcgctcc ggacttctgg tgcagtcgtc
     3061 atggccgata catatcattg gcagactggt gctcggtgcg ctggtgctgg tggtgatcag
     3121 ttttatcttg ctggcgcatt tctccggcgc cccgccgaat tcgacctaca ttcccgcgca
     3181 tatcgagaac ggcaagttcg ttcccggagt tgaaaaatga gcgatgcgcg cgtgctcgac
     3241 gacgcgccgt ggctcaaatc cggcccaacc gcccgcctgc tcggattgct gaacggcgat
     3301 ggcgaggaag cgcgcgtggt cggcggcgcg gtccgcaatg tgctattgca gattccgccc
     3361 ggcgatatcg atatcgcgac cacagccttg cccgatgaag tgatccgccg cgccagggcg
     3421 gccggcatca agagcgtccc gaccggcatc gaacacggca ccgtgacgct ggtcgtcgat
     3481 gggcacccct tcgaggtcac caccctgcgc gaggataccg agacgttcgg ccgcaaggcc
     3541 aaggtcgcgt tcggccgcga ctgggtccgc gacgccgagc ggcgcgactt caccatcaac
     3601 gggctgtccg ttgacgccgc cggtgtcgtg cacgatcacg tcggcgggct cgatgacatc
     3661 ctggccaaac gggtgcgctt catcggcgac cccgatcggc ggatcgccga agactatctg
     3721 cgcatcctca ggtttttccg cattcacgcc gcctacggcg tgggcgagcc cgatcgggat
     3781 gggtacctcg cctgcattcg cttgcgcgcc ggccttgcga ccctctccgc agaacgcgtg
     3841 cggatggaaa tgctgaagct ggtgatcgcc gacggtgctg ccggcgccgt cgcggcgatg
     3901 gcggatggcg gattgctgct gccgatttta ggcggcattg gctataccgg gccgttcgcc
     3961 gccatgatct cggcggagcg cctgcttgga ctgaagcccg acgcggttcg tcgcctcggc
     4021 gcgctcgcgg tcgccgtcac cgaggacgcc aggcgggttg cagcgcgctt gcggctctcc
     4081 aatgccgaaa caaaggcgct cgattccatg gggcatcgct ggtggcggct cggcggcatg
     4141 gacgaggcga tcgccaggcg acggctctat cggctcggcg aggaccgcta ccgtgaccgg
     4201 ctgatgctgg cgtgggcgcg ggcgggagcg ggcgccgatt ccgcgcattg gcgcgaactc
     4261 gccgccttgc cggagcgctg gcgggcgccg aaatttccgc tgaaggcggc tgatttcgtc
     4321 ccccgcggga tcaccgaagg tcccgcgctc gggcgggtgc tcgcgctggc cgaagacgcg
     4381 tggctggcgg cggatttccc gctggatgga cctgcgctca aagccattgc cgaccaaacc
     4441 gtcacccgct tcacccgcga ccatcggccg tgaacatcct ttccggtttt tccgatatct
     4501 cgatagctca actcctgctg gtgacatgcg tcgcgctgtt tgcctctgtc gtcggcgggc
     4561 tcgcgggtta cggcaccggc gcgctgatgc cgctggtgct ggtgccgctg gtcggcgccg
     4621 aaccggtggt gccgatcatc gcgatctccg cgatcttcac caacagcagc cgtttcgccg
     4681 cctatctgcg ttatgccgat ttccggcgcg cgggaatcgc ggccgcggcg gctgcggtga
     4741 cgaccgcgtt cggcgcttac ggatataccc ggctcagcaa cgccggcgcg gcgctagtga
     4801 tcggcgcgat gctgatatcg agcgtgccgc tgcgccgttt gctcaagcgc cgcgatgtca
     4861 aaatcggcga caccgggctc gccgccgggg cggtgggtta cggcgtcctg gtcggcggca
     4921 cttcgggctc gggtgtgatc ctgctgtcgc tgctgatggc ggcgggcctc gaaggcgcag
     4981 ccgtgatcgc caccgacgcg gtgatctcgg ttacgaccag catgatcaag atttccgtat
     5041 tcggactgag cggcgtggtc accgcgcaag tgctcgcgct tgccgttctg atcggcgcga
     5101 tcgcgctgcc cggtgcgttt ctcgcaaaag tttttgtcga gcgtatgccg gtccatattc
     5161 acaccgcgat cctcgatgtc gcggtgatca taggcggcgt ggtcatgatt tccgcggccg
     5221 cgcggcgttt ctggtagagc aaattccggg aatacgattt cccggaagcg atgccgccag
     5281 gtcaaaagaa ttcggcggca tcgcggggcg aagtcatgcc gccggctttt cctggattcc
     5341 ttatccccgt cctactcttc ctcttcttcc tcgtcgtcgt cttcctcttc gtcgtcgtcg
     5401 atttcggctg cctcaagcac ggctaatgaa agcgtttcac gaaacagttc gccttctgta
     5461 cccatccaca cgcacacgat gtcgtcgtcg tcgacttctg ccacagtcat cggatgaccg
     5521 ccggatttca gcacgacaac atcgcccggt ttcaaaccca tgatttcttt ccccaaagtt
     5581 gattgacatg attcatggcc gtcgatctgt cgagacagac cgatcacggc cacttcactg
     5641 tgacaggatt acccggttct gtgtccggtc tgtgtcggtt ccgaacattc cggcgggcag
     5701 tgactctgtt gcttaagata atggcaaaat agcaacactt ggcttgacac cacgtgggtg
     5761 ggccaccgct acggccactt cacttccggc ggcatcgacg acaggatcga atccacgttg
     5821 ccgccggtct tcagcccgaa gatggtgccg cgatcgtaga gcagattgaa ttcgacgtaa
     5881 cgtccgcgcc ggatcagctg ttcctcgcgg tcttgcgcgg tccacggcgc atcgaaattg
     5941 cgcctgacga gctcgggata gattttcagg aacgcgcgac cgacctcctg ggtaaaggcg
     6001 aggtcggcat tccagtcgcc gctgtcgtga tgatcataaa aaatgccgcc gatgccgcgg
     6061 gcctccttgc gatgcggcag atagaaatag tcatcgcacc acttcttgta tttgtcgtag
     6121 tcggcgacgc cgttgtgccc gccacaggcc gcctgcatcg ccgtatgaaa cgcaatggta
     6181 tcggcgtctt gctgggtccg ccgccggtcg agcaccggcg tcagatccga gccgccgccg
     6241 aaccatgcct tggtcgtcac cacgaagcgc gtgttcatgt gtacggcggg aacgtgcggg
     6301 ttgcgcagat gcgcgatcac cgagatgccc gacgcccaga aattcggatc ctcgctggcg
     6361 ccttgaactt gcgaacggaa ttcggggctg aattcgccgt gcaccgtcga acaatgcacg
     6421 ccgacctttt cgaacagccg tccgcgcatc accgacatca ggccgccgcc gccgggcttg
     6481 ccggtatggt cggtgcgatc ccagggcgtg cgcacgaaac gtcccgcgct gccgggataa
     6541 agcgatgccg gcgcatcgtc ttccagccgt tcgaaggcct ggcagatgtc gtcgcgcaag
     6601 ctttcgaacc acgatcgcgc gcgggccttg cgatcttcga tgtcgtctgt gttcatgccg
     6661 agttccatat ttcccgtggg ctcgcaatga cgacagaggc aagtctaacc ctgcgggcca
     6721 taataagcgc agctctcgtt gcaactgtca cggtggacgc tgacccgcgt gatcgggccc
     6781 gcgcgctgaa cccgatccca gatgaagcgc gagaggttct caagcgtcgg cggtccaagc
     6841 gcgtcgacct tgttgagcaa tttgtggtcg agcatcttcc gcacctcggt catgcttcgt
     6901 tcgagcaggc cgaggtccag caccatgcct gtcgcgggat cgggcgtgcc gcgcaccgtc
     6961 acctcggctc ggaacgagtg gccgtgaatc tcctcgctcg ccgcgcccag cgtcgttccg
     7021 cccagcgcat gggcggcctc gaaccggaac gattttgtca attcccacat ccttagaaat
     7081 ccggatctac ctgataccga gcgtcttatg cgtctgcagg ctgagccgcc attgcggatg
     7141 gcgcagacaa tagtcgatcg tgcgcgcggt gttctcgatc acgtccggtc cgtccatcgg
     7201 ctgcagcgag aaacgttcga aggcgagatc gacgaaagcc tccgggacgg catcggcttg
     7261 cggataaacc agcttgagtt catggccgcg ccggatcacg aggtcggcgc cggctttcgg
     7321 gctgacgcag atccagtcca tgccctcggg cggctcgatc gtgccattgg tctcgatgcc
     7381 gatttcgaag ccgcgcgtat gcagcgcatc gacgagggcc tcgtccacct gcagcaacgg
     7441 ttcgccgccg gtcaatacca cgtaacggtt ggcgccgatg ccggtccatt gcgcggcgac
     7501 ggtatccgca agttcgccgg ccgttgcata gcggccgccc cgcgtgccgt tggttccgac
     7561 aaaatcggtg tcgcaaaacc ggcacgtcgc ggtggcgcgg tcctgctcgc ggccgctcca
     7621 caagttgcag ccggagaacc ggcaaaacac cgcggcccgg ccggcatgcg cgccttcgcc
     7681 ctgcaaggtc agaaagattt cctttaccgc ataactcatc gggcctgctc cgcgatcgcg
     7741 gcgccggtct acagcaggac ccgggacaat gccactgcaa catcgcacga acgcaaatca
     7801 agccgaattc cgcgcttcgg ccgcgccgat cccgtgcgac tgcggagggc aggtcttgtc
     7861 atcacgtctc gtcaatcgac gcaagcgctc gccgcctctt ttcagatgcg ggttggtgaa
     7921 ggctgcggct caatatgagg acggaacgtg gttttttccg tgccgcagtc cgggcatctc
     7981 caactgggcg gaatggcggc gaacggcgta ccacccggaa tggcttgatc gggcgacccc
     8041 gcggccggct cgtaaatgaa atagcacccg ccgcacatga acttctggtg gcggatgtgc
     8101 ggtttgccca ccagcggaag tgaaaaatgg ccaacggcgg tggtggatgg cgtgaacacc
     8161 tgatctccca cggcaaggcg tccgccttcc cgcgcgatga gataaacgcc caattccttg
     8221 tgcccgaacg ctgcacgtag cgagcccggc agatcgaggt cccttcgccc ggtctccggg
     8281 ttgacgttgg tcgcgccgca tcggccgttg cgccggtcaa cccgaaaaag cccgtcgccg
     8341 atccggatgt cgctgccgac ccattcgaat tcttcccagg gtctcgcgcc gtcgatgtag
     8401 agattggcgc gaaaccggag cggatcgatc ttgatgcccc agtgctcttc aaggcttcgc
     8461 accgtcgcaa ggttgatcag cgagatcaca ttgtccggct tgtccatgaa atgtccgtcg
     8521 ggcgaacgaa ccagtgtcgg cgcgctgcga agtgccggca ccagctgcca aaaaatatcc
     8581 gcgacttttg cgcgcgcctc atcatcgttc aggtcggcaa cgatgagctg acgattgtcc
     8641 tgcgtgatgg tcagttgcag cgtctcgacg tcgagcgtcg tcctgacccg tgccaattgc
     8701 tcctcgagca tcagcatcac gaacagtccc ttcttgcccc atctggggtc gttgatgtcg
     8761 aagggagctc cggggcgcac cagcgcaaac acgcgatcgt gcggaaacgg cttcctggct
     8821 tccaactcaa cgcgaaccag cggctgcgcg ctcaatccct tgatcgggta gcggtagatg
     8881 ttcttcagaa ccggcattgg cgcgccacct tgtcgtgcga tgatctttcc tgagattgca
     8941 cgatacggtg acgggctacg tagagccggt cgctgtcgat gtcgtggggc cagatgacgc
     9001 ttcgcaggtg agggcggcag cagcgaagca aactggtcgg aaaacaatgc ggtcatatcg
     9061 gtccggggat cgtcgatggc gtcgtcgacc gcatccgaca gtcgcgcaat gcccttttcg
     9121 atctgcttcg gcgacaacgc ggcatagccg agaatgatgg cgcggctcgt aagggcggtt
     9181 tgccgccgga aatgcacgcg cgcggatgac agcgaataca cgccgatccg cgcgcgcagc
     9241 gccagcgcct cgaccgtcac ggcatcggga atgcccggtg gcagatgcca gaggacatgc
     9301 agtcctccgc tgtcgccatc gacaaggaca tcgccgaaat ttcgacgaag tgctgcgatg
     9361 agacaatccc ggttatccct gtaatgggag cggacgcgaa gcagatgggc gccatagctg
     9421 ccgttgtgca tgaagtcggc cagcgtcgcc tgttccagcc acgaattgcc gttattgagc
     9481 aggcatttct cgctgcggac ggcttcggcg atatgctcgg gcgccaccat gtagccgagg
     9541 cgcaatccgg cgccgagcga ttttgagaac gtgcccatat agaccgcgca atccggcgcg
     9601 agcgcggcca gcggcggaag atgcgatccc tggtagcgaa tatcgcaatc ataatcgtct
     9661 tcgataatgt agcagccgta ccgccgggcc cagtcgatca cctcccggcg tcgctccgcc
     9721 gacaaggtcg cgccggtcgg atactggtgc gagggcgtcg catagagcag cgaagccgcg
     9781 cgctgcggaa gatagtcggg gatcaatccg tcctgatcga ccgcgacgct tgcgatctcg
     9841 gcgcccgcgg cctcgaacgt cagcgccgcg ccgcgatagc agggatcctc cacgacgctg
     9901 agcgagccgc gcgcaaggaa aagtcgcgcc agcagggtga ggccttcctg gataccggat
     9961 atgatgacga tccggctggg atcggcgacg atgccgcgcg ccgctaccag atggttggcg
    10021 atcgcggtgc gcagggccgg aagtccagcc ggttcgccat attgggtgag acccaccgcg
    10081 ccgccgtgag aaagattggc ctgcagcagg cgccgccagg tcttgagagg aaacaggtcc
    10141 gcgccggggt ggcctggaaa gaagtcatac agcaggcgat tgcgggcggc atgggctgcg
    10201 ccttgcgtgc gtccctggcg caaaggcatc ggcatccgca cctgcatgat gtggtcgcgg
    10261 ggatcggacg ccggctgcat ggctgcggaa ttaagcggga gcggttcggc gacatagatg
    10321 ccggatgccg ggcgcgactc gacgatcccc tccatcagca atagatcata ggcgcgcatg
    10381 accgtgttgc gggatatcgc cagttgctcc gacaatcgcc gcgacgacgg catgcgcgtg
    10441 cccggcccaa ttcgcgcgca ccggatcgcg tcgcgaatct gatcgaccat ctgcgtcgtc
    10501 agcgagtccg gccgggaccg gtccagaaga accgaaatct gcatgtgcgc cgcgccggtt
    10561 gagttttcgt ggtccattgc cggagcaagg gctgggccag ttgtgggtcc agtacattca
    10621 attctgacgt aatttccgtg tgtgtcagcc gttttatcgg cctatgtcga ctggctccct
    10681 cgatttgcca ccaactggac ccatcgcggc ccatcagcac gcgcggctgc ccagaagtgg
    10741 tgcagcggct gcccgaaacc tcgcgctgtc gactatctgc tgagcaatcg gagaaaaccc
    10801 gcgccagtcg cggcgtccgc cgatggagtc ctcgcatttt atgccagtgg ctctcggtgc
    10861 gcgtcgttct gccgtgcgag cgtcgaggca ctgacacgca gaaggggagc cgcatcgcga
    10921 tgctcccgat catcgaacca cacaggagca ctcatgagca ccgttcgaca ccgctggctc
    10981 tccgccgcgg ccgccgttct cgccattctc ggaactggtg tgaaccaggc gaaggctgaa
    11041 gacgccatca agatcggaat actccactcg ctgtccggta cgatggcgat cagcgagacc
    11101 atcctgaaag acctgatgtt gatggaggtc gccgacgtca acgcaaaggg cggcctcttg
    11161 ggaaagaaga tcgagccggt cgtggtcgat ccggcgtcga actggcctct gttcgcggag
    11221 aaggcccgcg aactgctggt caaggacaag gtcgccgccg tgtttggatg ctggacgtcg
    11281 gtgtcccgca agtcggtcct gccggtgttc gaggaattga acggcctgct gttctacccg
    11341 ctcgaatacg agggtgagga agcctcctat aatatcttct atggcagttc cgttccggac
    11401 aacaaggcgg tgcccgcggt cgaatatctg atgagcaagg aaggcggcga ggtgaagcgc
    11461 ttcgtgctcg agggcaccga ttacgtctat ccccgcacca gcaacaagat catcagggcc
    11521 tacctgaaaa ccaagggtgt caaggatgaa gacatcatgg agaactatac gccattcggg
    11581 ttttccgact ggcagaccga agtcgcggcg atcaagaaat tcggctcggc gggaaagaag
    11641 acggcggtgg tctcgacggt gaacggcgac gccaacgtgc cgttctacaa ggaactcggc
    11701 aatcagggcg tgagggccac cgacattccg gtgatcgctt tctcggtcgg cgaggaagaa
    11761 ctggcgggcg tcgataccaa gccgctggtc gggcatctcg cggcctggag ctatttccag
    11821 tcggtgaaca cgccggagaa caaggcattc atcgcgaagt ggcgcgccta tgccaagaac
    11881 cccaggcgcg tcaccaatga cccgatggag tcggcctaca ttctgttcca catgtgggtg
    11941 caggcggtcc agcaggccgg cacgacggat gtcgaggcgg tccgtcaggc gatgatcggc
    12001 cagagggtca agtcaccatc gggctttgag gtcgtgatgg gcagcaatca tcacatggca
    12061 aagccggtca tgatcggtga gatccaggca gacggccagt tcagcatcgt ccatcagagc
    12121 aaggccttgc cgcccaaggc atggagcccc tatgtcgagg ccaataaagg caaggtcgcc
    12181 gactggtcct ggccgtgggt gtgcggcgga tgcacctcgc cgcggtttgc ggcgaactga
    12241 tctcgcccgc cttgaactgc cggtatgcgg tccggttacg gcgacatggt cggcgtaacc
    12301 ggacgtcgtt gctgcggacg tcgttgctgg agaccacgag aggcactcga cggtgcggct
    12361 acgcgccctc gcgtgtttgc cgcagcgcct cgcccaatgc catcgccgcc gccatcgcga
    12421 tattgagcga gcgcaagcct ggcttgatcg ggatcaccag ccgcgcgtcg gcggcggcaa
    12481 cgacctcgtc ggggaccccg gcggactcgc gcccgaacag caggatgtcg gcggcgccgt
    12541 agcgatgatc aagataggag tcgataccct tggttgtgaa caggatcagc cggtaacccc
    12601 cgtcgctacg ccattgttcg aattttggcc acgagtcgtg gcgggtgatg gtcacctgat
    12661 cgagatagtc catgccggcc cggcgaaagt gccggtcgga caccggaaaa ccggccggct
    12721 cgatgatatg ggcggccaca tccaggcagg cgcacagacg caggatcgtc ccggtgttct
    12781 gcgggatgtc gggctgaaaa agtgcaatct gcattcgctg atcgtccgct gatcgttcct
    12841 ggggtctcgc catgaaatgt ctcaataaat catcacgccg cgcggaatga gtgcattgca
    12901 cgcttgcgac ccgatagcgg gcttgcgctc ttccggcaag ggtgccaata gaacgattct
    12961 ggactgcttg ttccgccccg attggccggg aaaagtggtc tttctgccgc cgcgggggtc
    13021 gcgggcgccg gcagtctgct ccggtttcgt tgatgcgttg cctggggcgg ttccaccggc
    13081 tgcaacttga gaaagggttt ggaatcgtga cgacagcgtc ttcgatggat tcaacacgcc
    13141 gtgatttcct ttttgttgca acgggcgccg ccgctgcggt cggcggactg gctaccctct
    13201 ggcctttcgt ctcccaaatg aacccggacg cctcgacgat tgcggcgggc gcgccgatcg
    13261 aagtcgatct ggcgccgatc gccgaagggc aggacatcaa ggtgttctgg cggggcaagc
    13321 cgatctacat cagccaccgc accaagaagc agatcgacga ggcccgcgcg gtgccggtcg
    13381 caagccttcc cgatccgcaa gccgaccagg cccgggtcaa ggagggccac gaccagtggc
    13441 tcgtcgtgat cggcatttgc acccatctcg gctgcattcc gatcgcccat gaaggcaatt
    13501 acgacggctt cttctgcccg tgccacggct cgcaatacga ctcctcgggt cgggttcgcc
    13561 agggcccggc gccggccaac ctgccggtgc cgccctatca attcgtttcc gatagcaaaa
    13621 tccagatcgg ctgagcctcg gaccagatat ccgggacttg gagccatcgc gtcgttttac
    13681 ttcctcagga tcgcatcatg agcggaccat ccgactttca gccgtcccac cccgccttga
    13741 agtggattga gcggcgcctt ccgattatgg gtctcgtcca ttcttcgttc gtggcctatc
    13801 cgacgccgcg caatctgaac tactggtgga ccttcggcgc catcctttcg atgatgctcg
    13861 gcctgcagat cctgaccggc gtgatcctgg cgatgcacta cacgccggag gccaccatgg
    13921 ccttcaagtc ggtcgaaagc atcgtgcgcg acgtcaacta cggctggctg ctgcgcaaca
    13981 tgcacgccag cggcgcctcg atgttctttc tcgccgtcta catccacatg ttccgtggcc
    14041 tgtattacgg atcatacaag gagccgcgcg aaatcctctg gattctcggc gtcatcatct
    14101 acctcctgat gatggcgacg ggcttcatgg gctacgtgct gccgtggggc cagatgagct
    14161 tctggggtgc cacagtcatc accaatctgt tctccgccgt cccctatgtc ggcgacagca
    14221 tcgtgaccct gctgtggggc ggctattcgg tcggcaaccc gacgctgaac cgcttcttct
    14281 cgctgcatta tttgctgccg ttcgtgatcg ccggcgtggt cgtgctgcat gtctgggcgc
    14341 tgcatgtcgc gggccagaac aatccggccg gggttgaggc aaagaccgaa aaggacaccg
    14401 tgcctttcac gccttatgcg accatcaagg atgcgttcgg catctcctgc ttcatgctgt
    14461 tctttgcctg gttcatcttc tacatgccga actatctcgg cgacgccgac aactacatcc
    14521 ccgccaaccc ggcggtgacc ccgcagcaca tcgtgccgga atggtattac ctgccgttct
    14581 acgcgatcct gcgctcgatc cccaacaagc tcgccggcgt ggtctgcatg ttcggcgcga
    14641 tcatcatcct ggcgttcctg ccctggctcg ataacgccaa gacgcggtct tcgaagtatc
//
LOCUS       DATNOI010000425        12898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_999606,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000425 DATNOI010000000
VERSION     DATNOI010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12898
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999606"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..718
                     /locus_tag="VN968_17120"
     CDS             53..718
                     /locus_tag="VN968_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30990.1"
                     /translation="MNVYYKNTRIKQYHKEDRALRTETTINNSYDFGIGKRLHNLPKL
                     REIGFAANRRLLEVERLSHDCILAEDTFQAINSPVAAGRQRASGLRFADPRVHALWHA
                     IILFRQLAEGFRAADLRHHLAALSGRDPDAISQGAITYQLRRLRLHGLIERLPNSFRY
                     RVTEFGFRAALFFTRLYNRLLRPGLAAALPHLRAVDAPLKRAFDKINTQVQAWIDQAQ
                     FAA"
     gene            complement(815..991)
                     /locus_tag="VN968_17125"
     CDS             complement(815..991)
                     /locus_tag="VN968_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL30991.1"
                     /translation="MPGEPSAVLDRFNVGVELNSNPVSQRNAIFHIEENCIAITSLVL
                     VVSATENHRREAAI"
     gene            1273..2040
                     /gene="surE"
                     /locus_tag="VN968_17130"
     CDS             1273..2040
                     /gene="surE"
                     /locus_tag="VN968_17130"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HXL30992.1"
                     /translation="MRILCTNDDGIHAPGLKIVEEIARALSDDVWVVAPELDQSGVSH
                     SLSLNDPLRLREVGPRHFAVRGTPTDCVIMGARHILGPIMPDLVLSGVNKGRNVAEDV
                     VYSGTIAGALEGTILGIPSFALSQEFSMETRHAPLWETALQFGPRILRKVIDAGVPKD
                     TVINVNFPACAPEDVAGVRVTRQGKRNLGFLKVDQRRDGRGNPYFWIGFERLAQIDVP
                     AEGTDLAALAARYVSVTPLRLDRTDEAFSEALTATLK"
     gene            2496..4406
                     /locus_tag="VN968_17135"
     CDS             2496..4406
                     /locus_tag="VN968_17135"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HXL30993.1"
                     /translation="MSSEIPGHFRSTTRTRLRPDVPRGAEPRRRPARPDQKASKPPRP
                     KERIRKTDLGTILLHWILVITLTLSIGTGLRIAMDSPEHSWLVAFDSFLPLYTVWTLH
                     IPAALTLVAVSVAYAAYIWRGGLMRRIRFDKMRLKGLGNRSKQVRWGSINIILYWVLF
                     ATMIMEITTGTLLWLGYGRGLWVEMHMWGTWIILGYAAAHVMAHFALGGFNQLARVLR
                     PTKLGPRQKPFDPMDLVAELLDPENAAKSQAEPQPNSRWIFRLSPLVVALAAAAAGIA
                     IAVTVDGASEDTLFVRRIAKADRPTLDGDLSDGVWRTVRPFTMFTALGGNYDGKGEAA
                     VEIRAVHDGEWAYFCFMWDDPTRSLKHLPLVKQADGWHVLHDRYDLGDERSYYEDKFS
                     ALLTKLPIVIPGDRTYHSGPTPIEGKPSTLSQRGLHFIPAPGLAVEVWQWKASSGGLL
                     GWVDKNHFDMPAEATQAQSEGQVPYRGGFIQDPRTAELYSDNFERQPVGGYERPIQPK
                     RLPRNWKATYEALGRIDLDPDHSDAEASRWWMNMSESEPYSAALDAEIPVGTMIPGSL
                     ILVDDIDRRADVVGMARWAAGHWTLEVARKLAAEAPDVPISTGTYLRVAVFDHSQIYH
                     TRHIRPIRLELE"
     gene            complement(4470..4847)
                     /locus_tag="VN968_17140"
     CDS             complement(4470..4847)
                     /locus_tag="VN968_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL30994.1"
                     /translation="MTATRPSGGSVFLVEDEVMIRMMVADMLEELGYSVAAEAGEINE
                     AIRLAQCTEFDLAILDVNVNGKVISPVAELIKARNRPFIFATGYGSSGLPEEYRDRPA
                     LQKPFQLETLARMIESALKSATV"
     gene            5126..5776
                     /locus_tag="VN968_17145"
     CDS             5126..5776
                     /locus_tag="VN968_17145"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXL30995.1"
                     /translation="MPPTQNPPEKMMFQLNLRRRGISDQAVLRVMEEIPRDLFVEAAD
                     RADAYRDSALAIACGQTISQPFVVAYMTEQLQLQKHHRVLEIGTGSGYQAAILSRLCS
                     QVLTVERFRTLADNARLRLKKLGCGNVEVTLGDGFDIPASVGNFDRIMVTAAMQQIPD
                     SLTQRLEPGGILIAPVGPPHGVQTLIRMISTDTGFRCRELVDVRFVPALPGIAREL"
     gene            5963..7339
                     /locus_tag="VN968_17150"
     CDS             5963..7339
                     /locus_tag="VN968_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HXL30996.1"
                     /translation="MSRIVELLCSRRMPQVAVLALMSIGFAGCSGDMQTRLSQDSLSN
                     PFASEATGTAPPAPIERREPPRYPRPQPQPQYQYQSQALPPPIAVPQSNPTASAGVSG
                     GGRGLASYAPPARPPLETTGTVAPRSVAASHSAGQAAGQGATTIIVGTSDTLDTLSRR
                     YNVSPAAILQANGYRGPRTLSPGQQLIIPRPTAVVAAPAVAPAPALTAPASRAIAVAA
                     APTVHVVNHGDTLLSIARRNHVPIGELARANHLEPSAKIRLGMKLTVPGAKTAAAAPA
                     AQPAMAAVAVQPAAAVAAPASRMAAAGGTPQSARLAQATEDGADATPVKAAEATGALP
                     TFRWPVRGKVITSYGAKTNGKSNDGINLAVPEGTPVKAAEDGVVAYSGNELKGYGNLV
                     LVRHSNGYVTAYAHASELLVKRGDTIKRGQIIAKSGQSGEVGSPQLHFEIRKGSSPVD
                     PLQFLNGA"
     gene            complement(7478..8449)
                     /locus_tag="VN968_17155"
     CDS             complement(7478..8449)
                     /locus_tag="VN968_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL30997.1"
                     /translation="MSKKPSKTRPRAVSRASSRKLPTAAAKRPAAASKHDTADRIADA
                     LEAIAAHLSAASPLPNAADTFGSADAFVWHPDGRLAPVPRVSRVDLGLLKGIEQVRDI
                     LIENTERFADGLPANNALLWGARGMGKSSLVKAAHASINVNRKPAERLKLIEIHREDI
                     ESLPELMDLLRRSDFRFIVFCDDLSFDGNDASYKSLKAVLEGGIEGRPDNVILYATSN
                     RRHLLAREMIENERSTAINPGEAVEEKVSLSDRFGLWLGFHRCSQDEYLAMVKGYCGH
                     FGIQLAHDALEREALEWSTTRGSRSGRVAWQFTQELAGRLGVRLAGK"
     gene            8648..9013
                     /gene="yajC"
                     /locus_tag="VN968_17160"
     CDS             8648..9013
                     /gene="yajC"
                     /locus_tag="VN968_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXL30998.1"
                     /translation="MFITPAFAQAAGAGDTNSMLMSLLPFALIFVIMYFLILRPQQKK
                     VKDHAELVKNIRRGDIVVTSGGLVGKVTKVVDDDQIEFEISDGVRVRQMRQMISGVRA
                     KGEPAKEKSEPAKEDTPAS"
     gene            9063..10664
                     /gene="secD"
                     /locus_tag="VN968_17165"
     CDS             9063..10664
                     /gene="secD"
                     /locus_tag="VN968_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HXL30999.1"
                     /translation="MLYFTRWKALGIILIALVVCLCAVPNFFPEATVKTWPQWAQRHL
                     VLGLDLQGGSHLLLEVDANAVRKDKLDQVRDDVRRVLRENKIVYTGLASRADSVEVRI
                     TKDTDLPTALSKLRELSQPLGGLLGSSGQRSLDVSDAGSGLIRLTVPQAAVTDRIRQA
                     VEQSIQIVERRVNELGTVEPLIQRQGNDRILVQVPGLGDPSRLIAILGQTAKMDFRMV
                     DSTVSPDQALAGRVPPDSEVLPSAEASHQPYVIKKQVLVSGGDLTDAQPGFDQRSGEP
                     IVSFRFNTSGSRKFAQATSENVGQPFAIVLDNKVISAPVIREPITGGSGQISGNFTVQ
                     AANDLAILLRAGALPAPLTVIEQRTVGPGLGQDSIQKGELAAYVGSIMVIVFMLVTYR
                     LFGVFANIAVAINVAMIFGVLSLLNATLTLPGIAGIVLTVGIAVDSNVLIYERIREEI
                     RGGRTAISAIDAGFKRALSTILDSNITTFIAAAVLFYIGTGPVRGFAVTLGIGIITTV
                     FTAFELTRLIVAGWVMWKRPSSVPI"
     gene            10780..11784
                     /gene="secF"
                     /locus_tag="VN968_17170"
     CDS             10780..11784
                     /gene="secF"
                     /locus_tag="VN968_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HXL31000.1"
                     /translation="MTHLVLIGLGILIAVLTVVSCFGLLPSLRIVPDDTKFDFTRFRR
                     ISFPISALLSIVAITLFFTNGLNFGIDFKGGTLMEVQAKSGAADLASMRSTLSTLGLG
                     DVQLQQFGGPADVLIRVAEQPGGDAAQQAAVQKVRGALGDAVDYRRVEVVGPRVSGEL
                     LAYGMLGLMLAIFAILIYLWFRFEWQFALGAMIANVHDIVLTIGFMSITQVDFDLTSI
                     AALLTILGYSLNDTVVIYDRIREMLRRYKKMPMPELLNESINSTLSRSIITHVTVTLA
                     LLALLLFGGHAIHSFTAVMMFGVVLVGTYTSIFIAAPILIYLGVGTNRADMPDVPKKK
                     "
     gene            11800..12183
                     /locus_tag="VN968_17175"
     CDS             11800..12183
                     /locus_tag="VN968_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HXL31001.1"
                     /translation="MAVPPDAPHLPRSAPIEAYGRYGFAFAGMSHRGSLLCLPEAIWA
                     WPVTRPEDIDKASLARVFAAAGGIDTLIIGSGTDVWLPPPALREALRAVRVVLDAMQT
                     GPAIRTYNIMIGERRRVAAALIAVP"
     gene            12180..>12898
                     /locus_tag="VN968_17180"
     CDS             12180..>12898
                     /locus_tag="VN968_17180"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HXL31002.1"
                     /translation="MSGADGLADSADFCAQLVRTHDFARYASTLFVPAAERRALLAIY
                     AFNVEISRVREQVSQPLPGEMRLQWWTDMLAGEGHGGIEGNPVAAELQLAIQEWHLPV
                     ERLSRLIDEHQFDLYNDPMPTMAALESYLNDTASALFLLGAGIAGGQSGEIEHLARHA
                     GLAQGMAQVMAALPRDASRRQLFVPLQVLQQHGSGMEEVFAGRQTAKLRAVLHQLVGD
                     AQGHLKTAFALLATVPPRVRPV"
BASE COUNT         2317 a         4083 c         4026 g         2472 t
ORIGIN      
        1 gacaccggga cagttccgca cccgcatcgt cacccaggac gtcaccccgt ccttgaacgt
       61 ctattacaag aacacgcgga tcaagcagta tcacaaggaa gatcgggcct tgcgtaccga
      121 gacgaccatc aacaacagct acgatttcgg catcggcaag cgcctgcaca acttgccgaa
      181 gctgcgcgag atcggctttg cggccaatcg ccggttgctc gaagtcgaac gtttgagcca
      241 tgactgcata ctggccgagg ataccttcca agccatcaac agcccggtgg cggcgggccg
      301 ccaacgcgcc tcgggcctgc gttttgccga tccgcgcgtc cacgcgttgt ggcacgccat
      361 catcctgttc cggcagctcg cagaaggctt tcgcgcagcc gacctgcgcc atcatcttgc
      421 ggctctgtcg ggccgcgatc cggacgcgat ctcgcagggc gccatcactt atcagctgcg
      481 ccgcttgcgc ctgcacggcc tgatcgaacg cctgcccaac agcttccgct atcgcgtcac
      541 cgaattcggc ttccgggcag ccctgttctt cacgcgcctc tacaaccgtc tccttcgccc
      601 gggcttggcc gccgcacttc cacacctccg cgccgtcgac gccccgctca agcgcgcatt
      661 cgacaaaatc aacacacagg tccaagcatg gatcgatcag gcccaattcg ctgcttgaaa
      721 cttgacacat ttagtccatt tgtcctgatt caagctgggc tagagccccg gttcgattcg
      781 ttcaaaacaa ggcgcggcgc ctcggtaaac catttcatat ggctgcctcg cgccggtgat
      841 tttccgtggc cgaaacgacc agaacaagcg aggtgatggc gatgcagttt tcttcgatat
      901 ggaagatggc gttccgctgc gagaccggat tggagttgag ttcgacacca acgttgaagc
      961 gatcgagcac agccgagggc tcgcccggca cttccgcgac gagagtctcc cgcgacgacc
     1021 aggaccttga gatatccgtg gtcaatgcgc tgagacggga aattcatcgc gagttcgttc
     1081 accgggaagc ctgcggcaaa tagccatgcc ggcggcttcg gatggctttc aacgaccggg
     1141 cgggggtcga ttcggatacc ccagcgaaac cggccatttc gatcgcgttc gacgggtttg
     1201 cctcaacgcc gtgagcggga taagacgggg cattggccat tccggaatcg atcgacaagg
     1261 gcagccagac ggatgcgaat tctctgcacc aatgacgacg gcatccatgc ccccggcctg
     1321 aagatcgtcg aggagatcgc ccgcgcgctg tccgacgacg tttgggtggt ggcgccggag
     1381 ctcgatcagt ccggcgtctc gcattcgctg tcgctgaacg atcccctgcg gttgcgcgaa
     1441 gtcggcccgc gccactttgc ggtgcggggc acgccaaccg attgcgtcat catgggcgcg
     1501 cgccacatcc ttgggccaat catgccggat ctcgtgctgt cgggcgtcaa caagggccgt
     1561 aacgtcgccg aagacgtggt ctattccggc accatcgccg gcgcgctgga aggcacgatt
     1621 ctcggcatcc cgtcgttcgc gctgtcgcag gaattcagca tggagacccg acacgcgccg
     1681 ctatgggaaa cggcgctaca gttcgggcca aggatcctgc gcaaggtgat cgacgccggc
     1741 gtgccgaaag acaccgtgat caatgtcaac ttcccggcct gtgcgcccga ggatgtcgcg
     1801 ggcgtgcgcg tgacgcggca gggcaagcgc aatctgggat tcttgaaagt cgaccagcgc
     1861 cgcgatggtc gcggcaatcc gtatttctgg atcggtttcg aacggctggc gcaaatcgac
     1921 gtgcctgcgg aaggcaccga tcttgccgca ttggcggcgc gctatgtttc ggtgacgcca
     1981 ttgcgattgg atcgcaccga cgaggcgttt tcagaagcgc tgacggcgac actgaagtag
     2041 ggctttcatt tccccttgtg acttcgcaac agccagcgtt catcggatgt cggccgaaag
     2101 aacttgcagc ctgcacgctt tggcgtcacc acatccggat gtgctccctg ttccgagcgg
     2161 cgcgagaatt tgcattgatc tcaatcaagc cggacgcgca tgaattcgtc gatctttctt
     2221 caatcacatg gtcttgccgc actgcatcag cgaattgcct aattccgacc ccgaaattcg
     2281 ctcaatcaaa atatacaaat caagactggt tgaagaggtc atccttgtcc gcattgtgaa
     2341 gacgtagctg atcctgcgtc tggagcatcc ttctgcgtta cggcgtggaa tggatggcgc
     2401 ttgtcacaat gccagcagat gacttcgagt tgtagcgatc taggttcgat ggaccggcag
     2461 cggcatcagc gaacttctta agtcaggtaa ggccgatgtc ttctgaaatc ccaggccact
     2521 tccgcagcac gacccgcacg cgcctgcgcc ccgatgtccc ccgtggcgcg gagccgcggc
     2581 gtcggccggc gcgaccggat cagaaggcgt caaagccgcc gcgaccgaag gagcgaatac
     2641 gcaagaccga cctcggaacg atcctgctgc attggattct ggtgatcacg ctgacgctgt
     2701 cgatcgggac cggactccgg atcgcgatgg attcgccgga gcattcgtgg ctcgttgcgt
     2761 tcgacagttt cctgccgctt tacacggtgt ggacgctgca tattccggcg gcgctgaccc
     2821 tcgtcgccgt gtcggtcgcc tacgcggcct atatctggcg cggcggcctg atgcgccgca
     2881 tccgttttga caagatgcgg ctgaagggat tgggcaaccg cagcaagcag gtgcgatggg
     2941 gttcgatcaa tatcatcctg tactgggtgt tgttcgcaac catgatcatg gagatcacga
     3001 ccggcacgct gctctggctc ggctatgggc gcggtctctg ggtcgagatg cacatgtggg
     3061 gcacctggat cattctcggt tatgcggcag cccatgtcat ggcgcacttt gcgctcggtg
     3121 gattcaatca gctcgcccgc gtgctgcggc caaccaagct cggcccgcgg cagaagccgt
     3181 tcgatccgat ggatttggtg gcggaactgc ttgatcctga aaacgcggcg aagagccagg
     3241 ccgagccgca gccgaacagc cggtggattt ttcgcctcag cccgcttgtc gtggcgttgg
     3301 cggcagcggc cgcgggaata gcgatcgccg tgaccgtcga cggggcgtcc gaggacaccc
     3361 tctttgtccg gcgcatcgcc aaggccgacc ggccgacgct tgacggcgat ctgtccgacg
     3421 gggtctggcg aacggtcagg ccgttcacga tgttcaccgc gcttggcggc aattatgacg
     3481 gcaagggcga agcggcggtc gagattcgcg cggtccacga tggcgaatgg gcttacttct
     3541 gcttcatgtg ggatgacccg acccgatcgc tgaaacatct tccgctggtg aaacaggcgg
     3601 acggctggca tgtcctgcac gatcgatacg atctcggtga cgagcgatcc tattacgagg
     3661 acaagttctc cgcgctgttg accaagctgc cgatcgtgat tcccggcgac cgcacctacc
     3721 actccggacc gacgccgatc gagggcaagc catcgacatt gtcgcaacgc ggtctgcact
     3781 tcatcccggc acccggtctc gccgtcgaag tctggcaatg gaaggcgtcg agcggcggcc
     3841 tgctcggatg ggtcgacaaa aaccatttcg acatgccggc cgaggcgacc caggcccagt
     3901 cagagggcca ggtgccctat cgcggcggct tcatccagga tccacgcacg gcggagctct
     3961 atagcgacaa tttcgagcgc caacccgtcg gcggctatga gcggccgatc cagccgaaac
     4021 ggctgcccag gaactggaag gcgacctatg aggcgcttgg gcggatcgac ctcgatcccg
     4081 accacagcga tgccgaagcg tcgcggtggt ggatgaacat gtcggaatcc gagccctatt
     4141 cggccgcgct cgacgccgag attccggtcg gaaccatgat tccgggatcg ctgatcctgg
     4201 tagacgatat cgatcggcgc gctgacgtcg tcggcatggc tcgctgggcg gccggccact
     4261 ggacgctgga agtggcgcgc aagctggcgg ccgaggcccc cgatgtcccg atttccaccg
     4321 ggacctacct gcgggtcgcg gttttcgacc actcccagat ctaccacacc cgtcacatcc
     4381 gcccgattcg cctcgagctt gagtgatcag cagttgtcgt catccgggat gacgtcgatc
     4441 ggtcggctgt tgggttctcg tctaacgccc tagaccgtcg cgctcttgag cgcgctctcg
     4501 atcatccgcg ccagggtttc gagctggaaa ggcttctgca gtgccggacg gtcgcgatat
     4561 tcttccggaa ggcctgagga gccgtagccg gtcgcgaaga tgaacgggcg attgcgcgcc
     4621 ttgatcaatt cggcaacggg cgaaatcacc ttgccgttga cgttgacgtc gagaatggcg
     4681 aggtcgaact cggtgcattg cgccagcctg atcgcctcgt tgatctcgcc ggcctcggcg
     4741 gccacgctgt agccgagctc ttccagcatg tccgcgacca tcatcctgat catgacctcg
     4801 tcctcgacga ggaacacaga accgcccgac ggccgcgtcg cagtcatgat aatgtccctg
     4861 ctcgtcccaa gacttaaggt tcgcaaataa cgtcaatcgg cgcaatgtca acgtgtgcga
     4921 acgggcatgc cggccggccg ccatcgcgcc gtggcgggca gggagcagta ttgattgccc
     4981 gctcacaaac gccaaggtgc tatcccgggt tccatagccg gaacaccgat tcgtggggaa
     5041 gttttcctta agccgccggc gcatgttaca tcagcctgtt cggccaaggc gcgctagact
     5101 gatacctgat cttgacgaga ttgccatgcc gccgacccaa aatccgccgg aaaagatgat
     5161 gttccagctc aacctgcggc ggcgcgggat cagtgatcaa gcggtgctgc gggtcatgga
     5221 agagattccg cgcgacctct tcgtggaggc ggccgaccgc gccgatgcct atcgcgacag
     5281 cgcgctcgcc attgcctgcg ggcaaaccat cagccagccc tttgtggtgg cttacatgac
     5341 cgagcagttg cagctgcaga agcatcaccg cgtgctcgag atcggtaccg gctccggtta
     5401 ccaggcggcg atcctgtcgc ggctttgcag tcaagtactg acggtcgagc gtttccggac
     5461 tctggccgac aacgccaggt tgcggcttaa gaagctcgga tgcggcaatg tcgaggtgac
     5521 gctgggcgat ggtttcgaca tcccggccag tgtcggcaat ttcgaccgca tcatggtcac
     5581 cgcggcgatg cagcaaattc ccgactcctt gacccagcgg ctcgagccgg gcggaatcct
     5641 gatcgcgccg gtcggtccgc cgcacggcgt ccagaccctg atccgcatga tcagcaccga
     5701 caccggcttt aggtgcaggg aattggtaga cgtgcgtttc gtgccggcgc tgcccggcat
     5761 tgcccgggag ctgtaggggt aggaagcaac gccagatgct gttcgggacg cgcgcgccgg
     5821 acagcgcaag cgtgccgccg ggagcatgtg cgcctcaggc cgcttcgact acccgttaat
     5881 gccccatccg aagggttaag cggttgttta ctgggggtgt gtttactcaa aacagtatca
     5941 agttgcgtac gagtgagtaa ccatgtcccg aatcgtcgag ttgctttgct cgcgccgcat
     6001 gccgcaggtc gcggtgttgg cgctgatgtc gatcggtttt gccggctgta gcggcgacat
     6061 gcagacgcgg ctctcgcaag attccctgtc caatcccttt gcctcggaag ccaccggcac
     6121 ggcgccgcca gccccgatcg aacgccgcga accgccgcga tatccccgcc cgcagccgca
     6181 accgcaatac caataccagt cacaggccct gccgccgccg atcgccgtac cgcagtctaa
     6241 tccgacggcc agtgccggcg tttccggagg agggcgcggg ctggcttcct atgcgccgcc
     6301 cgcccgcccg ccactggaaa ccaccggcac cgtcgctccg cgctcggtag cggcaagcca
     6361 cagcgccggc caagcggcgg gccaaggcgc caccacgatc atcgtcggta ccagtgacac
     6421 gctcgatacg ctgtcccgcc gctacaacgt ttcgcccgcc gcgatcctgc aggccaatgg
     6481 ctacaggggg ccgcgcacgc tttcgccggg ccagcaactg atcattccgc gtccgaccgc
     6541 agtggttgcc gcacctgctg ttgcacctgc acctgcactg acagccccgg cgagccgggc
     6601 cattgcggtg gccgcagcgc cgaccgttca tgtcgtcaat cacggcgata ccttgctcag
     6661 catcgcgcgt cgcaatcacg tgccgatcgg cgaactcgca agagccaacc atctcgaacc
     6721 gtcagccaaa atcaggcttg gcatgaagct caccgtgccc ggtgcaaaga ctgcagccgc
     6781 cgcaccggct gcgcagccgg cgatggccgc tgttgccgtt cagcccgcgg ctgccgtagc
     6841 ggcgcccgcc agcagaatgg ctgcggcagg cggtaccccg caaagcgcgc ggcttgccca
     6901 ggccacggaa gacggcgcgg atgcaacgcc ggtcaaggcc gccgaggcca ccggcgcgct
     6961 gccgacattc cggtggccgg tgcgcggcaa ggtgatcacg agctatggcg ccaagaccaa
     7021 cggcaagtcg aatgacggca tcaatctggc agtgccggaa ggtacgccgg tgaaggcggc
     7081 cgaggacggc gtcgtcgcct attccggcaa tgagctcaag ggttatggca atctggttct
     7141 ggttcggcac tccaacggtt acgtcaccgc atatgcccat gcgagtgaac tgctggtgaa
     7201 gcgcggcgac acgatcaagc gcggccagat cattgccaaa tcgggtcagt cgggcgaggt
     7261 ggggtcgccg cagcttcact tcgagatccg caagggatct tccccggttg acccgcttca
     7321 attcctgaat ggggcgtgag gcgttccccg atacatcctg atcgtcattg ccgggcttga
     7381 ccgggcgatc cgtctccgag aattgctcgg tgcgaaggtt gatggatacg cgggtcaagc
     7441 ccgcgcatga cggtctgtgg ccttcgcagc agtattgcta cttccccgcc agccttaccc
     7501 ccagccgtcc cgccagttcc tgcgtgaact gccaggcgac gcggcccgaa cgcgagccgc
     7561 gcgtggtcga ccattccagc gcttcgcgct cgagcgcgtc atgcgcaagc tggatgccga
     7621 agtgaccgca atagcccttc accatcgcaa ggtactcatc ctggctgcag cgatggaagc
     7681 cgagccatag tccgaaacgg tccgacagcg acaccttctc ctcgaccgct tcgcccggat
     7741 tgatggcggt cgagcgttcg ttctcgatca tctcgcgcgc caaaagatgc ctgcggttcg
     7801 aggtggcgta caggatgaca ttgtcgggcc ggccttcgat gccaccctcc agcaccgcct
     7861 tcagcgactt gtaggaggca tcgttgccgt cgaacgacag gtcatcgcag aacacgatga
     7921 aacggaaatc cgaacggcgc agcaggtcca tcagctccgg cagactctcg atatcctcgc
     7981 gatgaatctc gatcagtttc aagcgctcgg cgggtttgcg gttgacgttg atgctggcat
     8041 gcgcggcctt caccagcgac gatttgccca tgccgcgggc gccccacagc agcgcgttgt
     8101 tggcgggcag gccgtcggcg aagcgttcgg tattctcgat caggatatcg cggacctgct
     8161 cgatcccctt gagcaggccg agatcgacgc ggctgacgcg cggcaccggc gccagccgtc
     8221 cgtcggggtg ccagacgaac gcgtcggcgc tgccaaatgt atcggctgca ttgggcagcg
     8281 gtgacgccgc cgacaggtgt gctgcgatgg cctcgagcgc gtccgcaatc ctgtccgcgg
     8341 tgtcatgttt ggaagctgct gccggacgtt ttgccgcggc cgtcggcaat ttgcgcgagc
     8401 tcgccctgga gacggcccga ggtctggttt tgctgggttt tttcgacatc ttgcgggttc
     8461 ctgaccacgc agccatatcg ggccaaatgg ccgaccgcaa gcggtgcaaa tgaggggtct
     8521 ggcggcgttg caattgggac cgcggccgct atagtccgcg cgaatttgac cggaccggcc
     8581 cgacccccga tgcgtcgccg gttcatggcc gtttcacgag gaatggccgt ttcacgagga
     8641 tgtccgaatg ttcataactc ccgcgtttgc ccaggctgcg ggcgccggtg ataccaacag
     8701 catgttgatg tcgctgctgc cgttcgcgct gatcttcgta atcatgtatt tcctgattct
     8761 gcggccgcag cagaagaagg tgaaggacca cgccgaactc gtgaaaaaca tccgccgcgg
     8821 cgacatcgtg gtgacgtcag gcggcctcgt cggcaaggtg accaaagtgg tcgacgacga
     8881 ccagatcgag ttcgaaatct ccgatggggt gcgcgtgcgc cagatgcggc agatgatttc
     8941 cggcgtgcgc gccaagggcg agccggccaa ggaaaagagt gagcccgcca aggaagatac
     9001 tccggcgagc tagcgcgttt tccttttcgc gcccattcct taagatctga cgggtcaact
     9061 cgatgttgta tttcacgcgg tggaaggcgc tggggatcat tctgatcgcc ctcgtggtgt
     9121 gcctttgcgc cgttccgaat ttcttccccg aagcgacggt gaaaacctgg ccgcaatggg
     9181 cgcagcgtca tctcgtgctc ggcctcgacc tgcagggcgg ttcgcacctt ctgctcgagg
     9241 tcgatgccaa tgcggtcagg aaagacaagc tcgatcaggt gcgtgacgat gtccgccgcg
     9301 tcctgcgtga aaacaagatc gtctacaccg ggcttgcatc tcgcgccgac agcgtcgaag
     9361 tgcggatcac caaggatacc gacctgccga ccgcgctgag caagctgcgc gagttgtcgc
     9421 aaccgctcgg aggtctgctc ggttccagcg gccagcgcag cctcgacgtc agcgacgccg
     9481 gtagcggatt gatccggctc accgtcccgc aagcggcggt caccgaccgc atcaggcaag
     9541 ccgtcgaaca gtcgatccag atcgtcgagc gtcgcgtcaa cgaactcggc acggtcgaac
     9601 ccctcattca aagacagggc aatgatcgca ttctggtgca ggtgccggga ctgggcgatc
     9661 cgtctcggct gatcgccatt ctcgggcaga ccgccaagat ggatttccgg atggtcgatt
     9721 cgaccgtttc accggatcag gccctcgcgg ggcgggtgcc gccggattcg gaagtcttgc
     9781 caagcgccga ggcgtcccac caaccctacg tcatcaaaaa gcaggtgctg gtctccggcg
     9841 gcgacctgac cgacgcgcag cccggtttcg atcaacgctc gggcgagccg atcgtaagct
     9901 tccgcttcaa cacctccggt tcacgcaaat tcgcccaggc cacgtcggaa aatgtcgggc
     9961 aaccctttgc catcgtgctc gacaacaagg tcatttcggc acccgtcatc cgcgaaccga
    10021 tcaccggcgg ctccggccag atttcgggca atttcacggt gcaggcggcc aatgatctcg
    10081 cgatcctctt gcgtgccggc gcgctgccgg cgccgttgac ggtgattgaa cagcgaaccg
    10141 tcggacccgg gctcggtcag gattcgatcc agaagggcga actcgccgct tacgtcggct
    10201 ccatcatggt gatcgtgttc atgctggtga cctaccggct gttcggcgtg tttgccaaca
    10261 tcgcggtcgc gatcaatgtc gcgatgatct ttggcgtctt gtcgctgctg aatgcgacgc
    10321 tgacgctgcc cggcatcgcc ggcatcgtgc tgaccgtcgg cattgccgtc gactccaacg
    10381 tgctgatcta tgagcgaatc cgcgaggaaa ttcgcggcgg ccgcaccgcg atttcggcga
    10441 tcgatgccgg tttcaaacgg gcgctgtcga cgattctgga ttccaacatc accaccttca
    10501 tcgccgcggc ggtgctgttc tatatcggca ccggcccggt gcgcggcttt gccgtgacgc
    10561 tcggtatcgg catcatcacc accgtcttca ccgccttcga gcttacccgc ctgattgtcg
    10621 cggggtgggt gatgtggaag cggccgagca gcgtgccgat ctaggcatga tcccgaaaag
    10681 tggacaccgg ttttcggagg agatcatgcc ttcacaagat aaagcgacga atatgaacca
    10741 gcctggctgg gtcatattcg ggggaatgcg gagccggctg tgacacattt ggtcctcatc
    10801 gggctgggca tcctgattgc cgtgctgacg gtggtcagct gcttcggcct gctgccgtcg
    10861 ctgcgaatcg tgcctgacga taccaagttc gacttcacgc gttttcgccg catcagtttt
    10921 ccgatttcgg cgctgctctc gattgtcgcg atcaccctgt tctttaccaa cgggctgaat
    10981 ttcggcatcg atttcaaggg tggcacgttg atggaggtgc aagccaaatc cggcgctgcc
    11041 gaccttgcat cgatgcgcag cacgctgagc acgctcggcc tcggcgatgt ccagctgcag
    11101 cagttcggcg gccccgccga cgtgctgatc cgggtcgccg aacagcccgg cggcgacgcc
    11161 gcgcagcagg cggcggtgca aaaggtgcgt ggcgcgctcg gcgacgccgt cgactaccgc
    11221 agggtcgaag tggtcgggcc gcgtgtctcc ggggaacttt tggcctacgg catgctcggt
    11281 ctgatgctcg cgatcttcgc gatcctgatc tatctctggt tccgattcga atggcagttt
    11341 gcgctcggcg ccatgatcgc caacgtccac gatatcgtgc tgaccatcgg cttcatgtcg
    11401 attacgcagg tcgatttcga tctcaccagc atcgcggcgc tgttgacgat tctcggctat
    11461 tcgctcaacg acaccgtcgt gatctatgac cgcatccggg aaatgctgcg gcgctacaag
    11521 aaaatgccga tgccggaact gctcaacgaa tcgatcaact cgacgctgtc gcgatcgatc
    11581 atcacccacg tcacggtaac gctggcgctg ttggcgctgt tgttgttcgg cggccacgcc
    11641 atccacagct tcaccgcggt catgatgttc ggcgtggtgc tggtcggcac ctacacctcg
    11701 atcttcatcg cggcaccgat cctgatctat ctcggcgttg gcaccaaccg ggccgacatg
    11761 ccggacgtgc cgaagaagaa gtagtattgg actgtagtga tggcggtccc ccctgatgcc
    11821 ccgcatcttc cgaggtcggc gccgatcgag gcctacggca ggtacggatt tgcgttcgcc
    11881 ggcatgtcgc atcgcggctc gttgctgtgc ctgccggagg cgatctgggc ctggccggtc
    11941 acgaggcccg aggatatcga caaggcctcg ctggcccgcg tgttcgccgc agcgggtggc
    12001 atcgacaccc tgatcatcgg ctccggtacc gacgtctggc tgccgccgcc ggcgcttcgc
    12061 gaggctttgc gcgcggtgag ggtggtgctc gacgcgatgc agaccggccc cgcgatccgc
    12121 acctacaaca tcatgatcgg cgagcggcgg cgggtcgcgg cggcgctgat cgcggtgcca
    12181 tgagcggcgc ggacgggctg gcggattccg ctgatttctg cgcgcaattg gtgcgaaccc
    12241 acgattttgc ccgctacgca tccacgctgt tcgtaccggc cgccgagcgc cgcgcgctgt
    12301 tggcgatcta tgccttcaat gtcgagattt cccgggtccg cgagcaggtg agccagccgc
    12361 tgccgggcga aatgcggctg caatggtgga ccgacatgct ggcgggtgaa ggccatggcg
    12421 gtatcgaggg caatccggtc gccgccgagc tgcagcttgc gatccaggag tggcacctgc
    12481 cggtcgagcg gctgtcgcgc ctgatcgatg agcatcagtt cgatctctac aacgacccga
    12541 tgccgaccat ggcggcgctg gaaagttacc tcaacgatac cgcctcggcg ctgtttttgc
    12601 tcggcgcagg catcgccgga gggcagtccg gcgagatcga acacctcgcg cgccacgccg
    12661 gcctcgcgca aggcatggcc caggtgatgg cggcattgcc gcgggacgct tcgcggcgcc
    12721 agttgttcgt gccgctgcag gtgctgcagc aacatggcag cgggatggaa gaggtgtttg
    12781 cgggcaggca aaccgctaag ctgcgtgcgg tgctgcatca gctggtcggc gatgcgcagg
    12841 ggcatttgaa gaccgccttc gcgctgcttg cgaccgtgcc gccgcgggtg aggccggt
//
LOCUS       DATNOI010000426        12612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_751152,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000426 DATNOI010000000
VERSION     DATNOI010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12612
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751152"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1874)
                     /locus_tag="VN968_17185"
     CDS             complement(<1..1874)
                     /locus_tag="VN968_17185"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HXL31003.1"
                     /translation="MHLKLDTSGSIDDGDVARDFGQDPADIVVLSAADSDLSAFGAAH
                     ASLPKDFPSVRLTNLLALGHPASVDLYVERTLCEARIVVLRMLGGESYWPHGVESLRS
                     DALRRDTLFACIPGEMDWNAALAARGTLGSDETYALWRYCSEGGVENAQLMLRLAAHL
                     IGRGEAPPPARPMPSAGFWQGEPASDNRPNAVVIFYRALVTGGDTAGIDALRTALLAR
                     GLNAVCLFVTSLKDERSAAFLRAALSAYPPDIFINATAFATATANEDAGILAMSDCPI
                     LQVAQAGISRANWEASTRGLNPRDLAMHVVLPEVDGRIFANAIAFKERGESRSGFAPT
                     AFQPVDDRVNATADLALAWVRLRRAERPARRVALVLANYPNRDGRLANGVGLDTPQSL
                     IDVLAAMRGEGYAIGDTPVDAAVMMKLLQDGPTNALDERASRIGGVAWPLAKYKAAFA
                     TLPDNVRRAIEARWGKVEQDPHVADGQFRLGLHRFGNIVVGVQPARGYNIEPKSTYHD
                     PDLVPPHHYLAFYLWLRREFDMHAVVHLGKHGNLEWLPGKSAGLSASCLPDAVLGQVP
                     HLYPFIVNDPGEGIQAKRRASAVIIDHLTPPMTRAELHDDLARLETLVDEYALAADLD
                     P"
     gene            complement(1892..2905)
                     /gene="cobW"
                     /locus_tag="VN968_17190"
     CDS             complement(1892..2905)
                     /gene="cobW"
                     /locus_tag="VN968_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HXL31004.1"
                     /translation="MSRVPVTVLTGFLGAGKTTLLRSLLTQADGRRIAVIVNEFGDAG
                     FDGGLVEECAAKACAPGDIVELTNGCICCTVADDFIPTMDKLLSRDRQLDAIVIETSG
                     LALPQPLLKAFDWPAVRTRATVDGVVTVVDALALSEGRVTIDEDAVAVQRAADDSLDH
                     DDPIEEVFEDQLACADLVVLSKSDLVSPAALAQIEARLKSMLRPGVNIVRSRGDLAPL
                     VLIGLNSAAEDDMAARAGHHGEEEEHDHDDFDSIVVMPSAAVSVDAMRARVSDVLSLT
                     GVLRVKGHARVADKAAPIVVQGVGGRVELAFARPDVKQVEHLVVIGLKGFDGDAVRRA
                     LEG"
     gene            complement(2917..3441)
                     /gene="cobU"
                     /locus_tag="VN968_17195"
     CDS             complement(2917..3441)
                     /gene="cobU"
                     /locus_tag="VN968_17195"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HXL31005.1"
                     /translation="MTALATTLVLGGARSGKSAFAEKMIGDSGLTRIYLATATADDDE
                     MRNRIVHHRMQRGDGWITVEEPLALVDALAREATHGRAVLVDCLTLWLSNLMFAERDP
                     DVEARRLMRFLGVAKYPVVLVSNEVGLGLVPETPLGRNFRDAQGRLNQIVAAAVPNVV
                     FIAAGLPLWLKRSS"
     gene            complement(3438..3806)
                     /locus_tag="VN968_17200"
     CDS             complement(3438..3806)
                     /locus_tag="VN968_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1636 domain-containing protein"
                     /protein_id="HXL31006.1"
                     /translation="MSVCITCKTADGAIVVGPDMFESVKAAMGESDRNVQVRPVQCLS
                     VCKRPATVAVSSSDGYTFLFGDLQTESGTAALVSFVQSYQKSDYGLVPWRERAEVLRK
                     GMVARVPPMCWSPDDGSAPK"
     regulatory      complement(4006..4199)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(4258..5721)
                     /locus_tag="VN968_17205"
     CDS             complement(4258..5721)
                     /locus_tag="VN968_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HXL31007.1"
                     /translation="MRALTLVHRWLGIPLCLLFAMWFASGIVMHFVPFPTLTEAERLD
                     GLAPIELPRVLHSPSEAVQASGIKDATRVRLLQRSDGPVFLVSAASGVAAFRAGNLSP
                     AALPTQGLALAVALDHARRRGMDVARAEVSALAAIDQWTVSGGFDPHRPLFRISLNDQ
                     PDTELYVSSTTGEVVRDTTRHERWWNYVGSVAHWIYPAALRSRPAVWNATVWSLSLAA
                     LITALAGSMLGILRVKMTGGRIDSPYQGWQEWHHVLGLVCMIFVLTWIFSGWLSMDSG
                     RLFSTGALTAEEAGKINSAPAWDTLPKDEWRSASVAVKEIEWFVFNGKFYRRERTGLD
                     AQRLFLDDSGATGAPAAPREFLESREVGAFVERLAPGCNAPIIVAADDNYGISSLMPH
                     APIYRSVCRDIWYQIDGASGAILERLDPSRRAYRWLYGALHTMDIPVLVAHPVVRSVL
                     IVLSCGFGFVFSITAILISLRRLGFPFSASRSSRPTP"
     gene            complement(5729..8065)
                     /locus_tag="VN968_17210"
     CDS             complement(5729..8065)
                     /locus_tag="VN968_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXL31008.1"
                     /translation="MAQGAPQAEKKPDSLGPVVVTSPQRKPLRRTESGTQRSRTAARV
                     AGRSRAATAVPVVAPVPGGGEVSHTPLNTNVVAESASRLGLTVRQIPAAVEVIDKLTI
                     EDRGLRTTTDVAQAAVGVTAGDAPGAPASFSMRGFTGTQINTLYNGIKIGPSEMTSRI
                     MDTANLDRVEILKGPASLLSGEGATGGAINYVNKVPHTGPITNDAFMGYDSFRGYRAG
                     YGSGGSTLIDGLDYRFDISHSNDRSFIDDTYSRLSNVSGQLNYRVTDNFKVWGAVEYK
                     QDKDRFYWGTPLVPANAPGIVPTSGIVSGTWSNYYLNGRTGVPNPVTIDARTLTTTYN
                     VLDNHSGANELWLRSGFQWDIASNVQLKSQVYVYNAQRHWFNNEINSFIDDPASPSFG
                     QVYRERLSVDHDQKLYGNVTDLTVNTVVAGFDNGFVATVAASSLQFNVVQDDFFNNDI
                     VNLVNPDRGLYGTQQTKNFYTHVDNVSLSLEDRIKLTSNFALIGGIRVEEIKLARTAF
                     DVKGALRDGYPFSTTFTPVTGRVGYTWEALPGMTFYSQYATAADPTVANIFNLRPTTP
                     LLLTTSRIYETGLKLLSSDKKAEFTVSAFDIERKNVYVPESGILFNVAGKIASKGFEA
                     AAAVNPFGGLRLWGNAAIVRSRFVNFDFIDGNGVSQSYSGMTPPNVPSFVANAGASYR
                     FETALPLELGASVRHVGDRFNFQDNFVTMNTYTLYDAYAFLDIPKSVFNGVNQTRIMF
                     RVKNLTNKLYAQWGDPGYTDQVILGAPRSYEVAASFKW"
     regulatory      complement(8351..8558)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8669..8941
                     /locus_tag="VN968_17215"
     CDS             8669..8941
                     /locus_tag="VN968_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31009.1"
                     /translation="MPSSKTPKWRDDIDALAFQPADHNGICMVHRRAFRTLLRAAPSP
                     QQCVDFFMAHQKAFQAAARAKVLRASVADTANFHLTSRDVTRQLKN"
     gene            8996..9178
                     /locus_tag="VN968_17220"
     CDS             8996..9178
                     /locus_tag="VN968_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017996633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB domain-containing protein"
                     /protein_id="HXL31010.1"
                     /translation="MQQSQTAHLPAIATDSSVVVQAVLAMALGLFVVGVVGFSHISAV
                     HNAAHDVRHANAFPCH"
     gene            9185..9901
                     /locus_tag="VN968_17225"
     CDS             9185..9901
                     /locus_tag="VN968_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HXL31011.1"
                     /translation="MPVFRSIVFSAALAGLIVGAVISVAQFLGTVPLIQKSEVYERKA
                     VAEAPPAAHEHAGAAHHHDNARSNDSEWEPEDGFQRNAFTVAANILTAIGFALLLTGI
                     YAIRGRPVMWREGLLWGLCGFVVFTAAPGLGLPPELPGMPVAELTARQIWWIATAAAT
                     AAGLCLLAFRPAAWAAILGLGLIALPHLIGAPLAPESHSEVPAALSHRFIVVVTLTSL
                     LFWGLLGVSTSIAFGRISRP"
     gene            10363..>12612
                     /locus_tag="VN968_17230"
     CDS             10363..>12612
                     /locus_tag="VN968_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HXL31012.1"
                     /translation="MPIVAASGFAGLGYEIVWTRQLSLALGSEMMAVLGAVAGFFAGL
                     ALGAFVLDRPIRRARSPRTAYVLLEAVIGLWGLICVWLLPVAGRALSPLLGTEPAPAL
                     LWAASFALPTLVLLPATAAMGGTLTALERIVREARGDPRVSAGIYGANTLGAVAGTLI
                     STFLLLPAWGLSGTLICLAGVNAVCALGMLALGSAAGRKSVEATEEVVQIGGPRLTIT
                     LFATGLLGIAFEILVVRLAAQVMQDTIYTFAGLLAAYLLGTAAGGLAWQRAGRRVQDA
                     SLGGLLAGTALACLTTAFLTPYIARIAETAVEGGLAGELVVAMALFLVPSAAMGALFG
                     LLAQRTRDQRGSLGWAVGINSVGASVAPLLTAQVVIPTLGAWTAIIPVALGYLLLIPL
                     RRAAFVWAAAPALVAVALWVRPAPSLTRVPPGGALLAVREGPMVTASVVDDAFGARYL
                     EVNGRFRMGGTSSVRSDHRQAMLPLLLHPAPHRALFLGIGTGATLVGGSQMPGVNVHG
                     VELSREVVELLPWFADPGAPAAAPRVTVADARRFIAADTTQYDVIIADLFHPALDGSG
                     ALYTTDHFETVRRRLAQGGVFCQWLPLYQLDLPSLRAIVRGFLEIYPSGSAWLNHYSV
                     RTPMLALIGPRDGGTLDLDALDTRLRDPAVRPVARRLGFETSMDLLGQYLGGARTLSA
                     FAGQGPRNTDDYPFVTFDARRNVQALTAPPWALLRAVTQALRPDPAELLAEPTRDALS
                     ARLAAYWRAR"
BASE COUNT         2340 a         3982 c         3928 g         2362 t
ORIGIN      
        1 ggatcgaggt ctgcggccag cgcatactca tcgaccagcg tttccagacg cgccaggtcg
       61 tcatgcaact cggcacgggt catgggcggc gtcaagtgat cgatgatgac ggcactggcg
      121 cggcgcttgg cctggatgcc ttcgccggga tcgttgacga taaagggata gagatgcggc
      181 acctgtccaa gtaccgcatc cggcaggcaa ctggcggaca gaccggcact cttgccgggc
      241 agccattcga gattgccgtg cttgccgaga tgcaccacgg catgcatgtc gaactcgcgc
      301 cgcagccaca ggtaaaacgc gagatagtga tgcggcggca ccagatcggg atcgtgatag
      361 gtgctcttgg gctcaatgtt gtatccgcgc gccggctgca cgccaacgac gatattgccg
      421 aaccgatgca ggccaaggcg gaactggccg tcggcgacat gcgggtcctg ctccactttg
      481 ccccatcgcg cctcaattgc gcgccgtaca ttgtccggca gcgtggcgaa cgcagcctta
      541 tattttgcca gcggccacgc cacaccaccg atgcgcgacg cacgttcgtc gagcgcgttg
      601 gttggaccat cctgcaacaa cttcatcatc acggcggcat caacgggcgt atcgccgatg
      661 gcatagcctt cgccacgcat tgccgcgagc acatcgatca ggctttgcgg cgtatcgaga
      721 ccaacgccgt tggcgagccg tccgtcacgg ttgggataat tggccagcac aagcgcgaca
      781 cggcgggcgg gtctttcggc ccgacgcaat cgcacccagg ccagcgcgag atcggctgtc
      841 gcgttgacac gatcgtccac cggctggaat gccgtcggcg caaagccgga gcggctctcg
      901 ccgcgttcct tgaaggcgat ggcattggca aagatgcggc catcgacttc cggcaacacg
      961 acatgcatgg cgagatcgcg cgggttaagc ccccgtgtcg acgcctccca gtttgcacgc
     1021 gagattccgg cctgcgccac ctgaaggatc gggcagtcgg acatcgcgag aatgccggca
     1081 tcttcgttgg cggtcgcggt ggcgaacgcg gttgcgttga taaaaatatc aggcggatac
     1141 gcggagagcg ccgcacgcag gaacgcggcc gaccgttcat ccttcaggct ggtgacgaac
     1201 aggcataccg cgttgaggcc tcgcgcaagg agtgcggtcc gcagcgcatc gatgccggcg
     1261 gtgtcgccgc ccgtgaccaa ggcgcggtag aagatgacga cggcattggg cctgttgtcg
     1321 ctggcgggtt caccctgcca gaagccggcc gacggcatcg gccttgccgg cggcggcgcc
     1381 tcgccgcgac cgatcagatg cgccgcgaga cgcaacataa gctgcgcatt ttcgacgccg
     1441 ccttcgctgc aatatcgcca gagtgcgtag gtctcatcgc tgccgagtgt cccgcgcgcc
     1501 gccagcgctg cgttccagtc catctcgccg ggtatgcagg caaacagagt atctcgccgc
     1561 agcgcgtcgc tgcgcaggct ttcgacacca tgcggccagt agctttcgcc gccgagcatc
     1621 ctgagcacca cgatcctggc ttcgcacagc gtacgctcga catagagatc gaccgaagcc
     1681 ggatgcccga gcgccaacag attggtcagc cgtacgctgg gaaaatcttt cggcaaggac
     1741 gcatgcgcgg caccgaacgc cgaaagatcg ctgtccgccg ctgacagcac gacgatatcg
     1801 gcggggtcct gcccgaaatc gcgagcgacg tcgccatcgt cgatactgcc gctggtgtca
     1861 agcttcagat gcatgcgcgt agaagcccct gctaaccctc caacgcacgg cgtacggcgt
     1921 cgccatcaaa tccctttagc ccgatcacca cgagatgctc gacctgcttc acatccggcc
     1981 gcgcaaatgc gagttcgacc cggcctccga ccccctgcac cacgatcggg gccgccttgt
     2041 cggcgacgcg ggcatggccc ttgacgcgca gcacgccggt gaggctcagc acatcgctga
     2101 cacgcgcgcg catcgcatcg acgctgacag ccgctgacgg catcaccacg atactgtcga
     2161 aatcgtcgtg gtcgtgttct tcctcctcgc catgatggcc ggcccgcgca gccatgtcgt
     2221 cttcagcggc ggagttcagc ccaatcaaca ccagcggcgc caagtcgccc ctggagcgca
     2281 cgatgttgac gcccggccgc agcatggatt tgagacgggc ctcgatctgc gccagggcgg
     2341 ccggcgaaac gagatcgctc ttcgacagca ccaccagatc ggcgcaggcg agttgatcct
     2401 cgaatacttc ctcgatcgga tcgtcgtgat cgagcgagtc gtccgcagcc cgttggacag
     2461 caacggcatc ctcgtcgatc gtgacgcgcc cttcggacaa agccagcgcg tcgaccaccg
     2521 tgacgacacc gtcgacagtg gcgcgggtgc gcaccgcggg ccagtcgaag gctttcagca
     2581 gcggctgcgg caatgcaaga ccggaggtct cgatgacgat ggcgtcaagc tgccggtcgc
     2641 gcgatagcag cttgtccatg gtcggaatga aatcgtcggc gacggtgcag cagatacagc
     2701 cgttggtgag ttcgacgata tcgccgggtg cgcaggcttt cgccgcgcac tcttcgacca
     2761 gccccccgtc gaaaccggcg tcgccgaatt cattgacgat cacggcgatg cggcggccat
     2821 cggcctgcgt caacagggaa cgcagcaacg tggtcttgcc ggcgccgaga aatccggtga
     2881 gcacggtgac ggggacgcga gacattccag ttctccttaa gacgaacgct tgagccagag
     2941 cggaagcccg gctgcaatga acacgacatt gggaacggcg gcagcgacga tttggttcag
     3001 gcgcccctgg gcgtcgcgaa agttgcggcc aagcggggtt tccggcacca ggccaaggcc
     3061 gacctcgttg gacaccagca ccacaggata cttcgccacg ccaagaaatc gcatgagccg
     3121 gcgcgcttcc acgtcgggat cgcgctccgc gaacatcagg tttgacaacc acaatgtcag
     3181 gcagtccacc agtacggcac gcccatgggt cgcctctcgc gccagcgcat cgaccagcgc
     3241 cagcggttcc tcgacggtga tccagccatc gccacgctgc atacggtgat gcacgatacg
     3301 atttcgcatc tcgtcatcat cagcggtcgc cgtagcaagg taaatccgcg taagtccgct
     3361 atcgccgatc atcttctcgg caaaagcgga tttgccggag cgcgcgcccc ccagcaccag
     3421 cgtggtggca agcgctgtca tttcggcgcg cttccatcgt ccggtgacca gcacatcggc
     3481 ggcacccgcg ccaccatgcc cttgcgcagc acctcggcgc gctcgcgcca tggcaccaga
     3541 ccatagtcgg atttttggta ggactgcaca aaggacacca aggccgcggt gccactctcg
     3601 gtctgcagat cgccgaacaa gaatgtgtaa ccgtccgaac tcgacaccgc gaccgtcgca
     3661 ggccgcttgc agacgctgag acattgcacg ggccgcacct gcacgttccg atcggattca
     3721 cccatcgcgg ccttcacgga ttcgaacatg tccgggccga ccacgatggc gccgtcggcg
     3781 gtcttgcagg taatgcacac gctcacgaca gtgaccgcgc tcggcgcgtt tgcgcgcgat
     3841 ggctcgacca atgtcaattc agcaggctct cgatccgcgt tttcgcgatc catcataggc
     3901 tcacaccagg gccgctagcg gccgcgagct ttgttgggtc gcgccgacag caaacgcgcc
     3961 gtccggctcg ctcccgtccg gtgcaccccg ccccgacgac gcctccaaag cacagttcgt
     4021 gatggcaggt ctcctggctc gcgggtcgct gcctgatcgc cgccttcccg gggaatcccc
     4081 agtggcaccg gcgagggctc gtcgcttaca gttgcggggg cagcgccggc gttgaaaaca
     4141 ctcgcaccgg cttccctttt caccttccac gcggaaggac catcactggg aacgctagcc
     4201 gccggggtcg ctgcggtcaa cccgatgtaa ggatgagaca tgatggagat tccgggatca
     4261 tggcgttggc cgtgacgacc gggaggcaga gaatgggaac cccagtctgc gcaagctgat
     4321 aaggatggct gtgatactga aaacgaagcc gaaaccacac gacagcacaa tcagcacgct
     4381 ccgcaccact ggatgagcga ctaacacagg aatatccatc gtgtgtaacg cgccgtagag
     4441 ccagcgatag gcccgccgcg atggatcaag cctttccaga attgcgccgc tggcgccatc
     4501 gatttgatac cagatgtccc ggcatacgga tcgatagatc ggtgcatgcg gcattaatga
     4561 ggagattccg tagttatcgt cagcggcaac aatgatggga gcgttacagc cgggcgccag
     4621 gcgctcgaca aacgcaccga cctcgcggga ttccagaaat tcccgtggcg cggccggcgc
     4681 tccggtagca ccggagtcgt ccaaaaacag gcgctgcgca tcaaggcccg ttcgttcacg
     4741 ccggtagaat tttccgttga agacaaacca ttcgatttcc ttgacggcta ccgaagcgga
     4801 ccgccattcg tctttcggca aagtatccca cgccggcgcg ctgttgatct tccctgcttc
     4861 ctcggctgtg agcgcaccgg tcgagaacag ccggccgcta tccatcgaga gccagccgct
     4921 gaaaatccag gtcaacacga agatcatgca cacgagcccg agaacatggt gccactcttg
     4981 ccaaccctga tacggggaat cgatccggcc gccggtcatt tttaccctga gaatgccaag
     5041 catgcttccg gcgagtgctg taattaacgc agcgagggaa agcgaccaca ccgttgcgtt
     5101 ccacaccgcc ggcctgctgc gcaatgcggc gggataaatc cagtgcgcga cgctgccgac
     5161 atagttccac catcgttcgt ggcgcgtcgt atcgcgcacc acctcaccgg tcgtcgatga
     5221 aacataaagc tcggtgtcgg gttgatcatt gagcgagatc cgaaacaggg ggcggtgcgg
     5281 atcgaaaccg ccggacacgg tccattgatc aatcgcggcg agcgcggaaa cctccgcccg
     5341 cgctacgtcc attccgcgcc tgcgggcgtg atccagtgcc acagccagcg ccagaccttg
     5401 cgtcggcaag gccgcgggcg aaagattgcc ggcgcgaaac gcggccacgc cggatgcggc
     5461 ggacaccaga aaaaccggcc catcgcttcg ttgcagcaga cgcacgcggg tggcgtcctt
     5521 gatgccgctg gcctgcacgg cttcgctcgg gctgtgcaat acccggggca gctcgatcgg
     5581 cgccagcccg tctagtcgct ccgcttccgt cagagtggga aacggaacaa agtgcatgac
     5641 gatgccgctg gcaaaccaca tcgcgaacaa caggcaaagc gggataccca accagcggtg
     5701 aaccagtgtc agtgcgcgca tgtacggctc accacttgaa cgaagcggcc acctcatagc
     5761 tgcggggtgc gccgaggatg acctggtcgg tgtatccggg atcaccccac tgggcgtaga
     5821 gcttgttggt cagattcttc acccggaaca tgatccgggt ttgattgacg ccgttgaaaa
     5881 ccgacttcgg aatgtcgaga aaagcgtagg cgtcgtagag cgtataggtg ttcatggtga
     5941 cgaagttgtc ctggaaatta aaccggtcgc caacatggcg gaccgatgcg cccagttcca
     6001 gcggcaaggc ggtttcgaag cgataggatg cccctgcgtt ggcgacaaag ctcgggacgt
     6061 tcggaggggt cattcccgaa taggattggg acaccccgtt gccgtcgatg aagtcaaaat
     6121 tgacaaaccg cgaccgaacg atggcggcgt tgccccaaag cctcaatccc ccgaacggat
     6181 tcaccgcggc ggcggcctcg aagcctttcg atgcgatctt tccggcgacg ttgaacagga
     6241 tgccgctttc cgggacatag acgttcttgc gttcgatatc gaacgccgac accgtgaatt
     6301 ccgctttctt gtctgaagaa agcaatttca ggccggtttc ataaatacgc gatgtcgtca
     6361 gaagcagcgg cgtggtcggg cgcagattga aaatgttggc gaccgtggga tcggcagcgg
     6421 tggcatactg gctgtagaag gtcatccccg gcagcgcttc ccacgtgtag cccacgcgac
     6481 ccgtcaccgg cgtgaaagtg gttgagaacg ggtagccatc acgaagcgcg cccttaacat
     6541 cgaacgcggt gcgcgccagc ttgatctctt caacgcggat accgccgatc agggcgaaat
     6601 tcgatgtgag cttgatccga tcctcgagcg acagcgaaac attgtcgaca tgggtgtaga
     6661 aattcttggt ttgttgcgtg ccatagaggc cacgatcggg gttgaccaga ttgacgatat
     6721 cgttgttgaa gaaatcgtcc tggacgacgt tgaattgcag actgctggcc gcaacggtcg
     6781 ccacgaaacc gttgtcgaat ccggcaacga cggtattcac tgtcagatcg gtgacattgc
     6841 cgtagagttt ctggtcgtgg tcgaccgaaa gccgctcacg atagacctgt ccaaagctcg
     6901 ggcttgcggg atcatcgatg aacgaattga tctcgttgtt gaaccagtgg cgttgagcat
     6961 tgtagacata aacctggctt ttgagctgga cgttgctggc gatgtcccac tggaaaccgc
     7021 tgcgcagcca aagctcgttc gccccgctgt gattgtcgag cacgttgtag gttgttgtca
     7081 gggtgcgagc atcgatcgtg accgggtttg gaacgccggt gcgcccgttg aggtaataat
     7141 tggaccacgt ccccgaaacg attccgctgg tcgggacaat gccgggtgca ttggccggca
     7201 ccagcggtgt tccccaatag aaccggtcct tatcctgctt gtattcgacc gcgccccaga
     7261 ccttgaaatt atcggtcacc ctgtagttca gctggcccga cacattgctg agcctggaat
     7321 aggtgtcgtc gatgaaactc ctgtcgttcg aatgactgat gtcgaaacga taatccagtc
     7381 cgtcgatcaa cgtgcttccg ccggagccat atcccgcgcg ataacccctg aatgaatcat
     7441 agcccatgaa ggcatcgttt gtgatcggcc cggtgtgcgg caccttgttg acatagttga
     7501 tcgcaccgcc ggtcgcaccc tcgcccgaga gcaatgaagc cggacctttc aggatctcca
     7561 cgcgatcaag gttggccgta tccatgatgc ggcttgtcat ttccgacgga ccgatcttga
     7621 tgccattata aagcgtgttg atctgggtgc cggtaaagcc ccgcatggaa aacgaggccg
     7681 gcgctcccgg cgcgtcgccg gcggtgacgc cgaccgccgc ctgcgccacg tccgtggtcg
     7741 tccggagacc gcgatcctcg atcgtcagct tgtcgatcac ttcgaccgcc gcgggaatct
     7801 gccgcacggt gagccccaac cgggaagcac tttcggcgac aacattggtg ttgagaggtg
     7861 tatgggaaac ctcgccgccg cccgggaccg gcgcgacaac gggcacggct gtcgctgcgc
     7921 gggagcggcc ggcaacgcgc gcggcggtac gactgcgctg cgttcccgac tcggttctgc
     7981 gcaacggctt gcgttgcggc gacgtgacga ccacgggtcc gagactgtca ggctttttct
     8041 ccgcttgcgg agcgccctgt gccatgaccg gagccgagcc gccgaaagca aaaagagcga
     8101 taaggccgag cggcgcgacg gacgaactca accagcgctt gatcgtgaca cagtggctct
     8161 gattggattg aagacgcgcg cacgaaacac gtggcgcagg atggccatga ccgcagaaat
     8221 tcgacacgct accgctcaac ggcgaagtac ttacccgtca gcgcggttgc cgctacattc
     8281 tgccttcgcc cctagcggca gacgtaccgg ctactcctgc ggcatcggag caccccgccc
     8341 gacgcgctcg cgcgtgacca tgaccggcag caggtttcct ggctcgcggg tcattgcctc
     8401 ggaccacctt cccagagact ttttcgatct ccagtggcat ttatggtctt cggctaaccg
     8461 cctacagttg cgggggcagc cgcggattta tgcacctgag tgcacgcacc gcattccctt
     8521 ggttagccct tgcgggcact accaatcacg aacttggcat ctgaccgtga cgagagtcaa
     8581 ccgttgaatc tgagtccact ttggggcgcc atccttgcta gacagcaagc cccgacaagc
     8641 tgtcgccgtc catgcagatg atcgactgat gccatcaagc aaaacgccga aatggcgtga
     8701 cgatatcgat gcgctggcat tccagccagc ggatcacaac ggcatctgca tggtgcaccg
     8761 tcgcgcgttc cggacgctgt tgcgggcggc gccgtcgccg caacaatgcg tggacttctt
     8821 catggcacac cagaaggcat ttcaagcggc agcccgcgca aaggtgcttc gtgcgagcgt
     8881 cgcggacacg gcgaactttc acctcaccag ccgggatgtc acccggcaat tgaaaaatta
     8941 gggtcgcgcg ggctgtcgtt ctcggctaaa atgatggcct tcgacgagga tcgacatgca
     9001 acagtcccag accgcccatc tccccgctat cgccaccgat tccagcgtcg ttgttcaagc
     9061 cgtactggcg atggcccttg gccttttcgt tgtcggcgtc gtcggcttct cgcacatcag
     9121 cgcggttcac aacgccgctc acgatgtccg ccacgccaat gcgtttccct gtcactgacg
     9181 acagatgccg gttttcagat ctatcgtgtt ttcggcagcg ctggcggggc tgatcgtcgg
     9241 cgcggtgatt tcagtggcgc aattcctcgg aacggtgccg ctgatccaga agagcgaagt
     9301 ttacgagcgc aaggctgtgg ccgaggctcc accggctgca catgagcatg ctggcgccgc
     9361 ccatcatcac gataatgccc gcagcaatga cagcgaatgg gagcccgaag atgggtttca
     9421 gcgcaacgcc ttcacggtcg cggccaacat cctgactgcg atcggtttcg cgttgctgct
     9481 caccgggatc tatgcgatcc gcggccggcc ggtgatgtgg cgggaaggcc tgctgtgggg
     9541 gctctgcggc tttgtggtgt ttactgcggc gcccggcctt ggtctgccgc cagaattgcc
     9601 cggcatgccg gtggccgaac tgacggcgcg gcagatttgg tggatcgcca cggctgctgc
     9661 aacggccgca gggctctgcc tgctggcttt ccgccccgca gcatgggccg cgattctcgg
     9721 ccttggtctg atcgcgctgc cgcatctgat tggcgcgcct ctcgcgccgg aatcccacag
     9781 cgaggttccg gccgcgttgt cgcacaggtt catcgtggtg gtgacgctca ccagcctgct
     9841 gttctggggt ttgctcggcg tctccaccag catcgcattc ggacgtatct cgcgcccgta
     9901 agaacgaagg aaatacgggc ctgtctcggt tcgaactttg tgctgcgctc agcgcagaac
     9961 aatatccgcc agaaagagac tgaacgccac gatggcggcc caggtccgtg cggtatcaag
    10021 ccgctcccat ctgtggatca tggatcacca tccctcgggc ggcgctgtgg cgacgccagc
    10081 cagccgttga cgcttttcga acatatcccg agcattgggt cgcccgccga cggagaatcg
    10141 cgcagatcga tatggcagtc aaaccagtgt cacaaattcc gggagataac tgcttgacgc
    10201 ggcaaacgca gtcgcaccta tccgcgaaca aggacgacaa tgatacaacc cgatccggac
    10261 agcggcgcga tggcgtcggg cgatcggttc gcgccggaag ctcgagccgg cgcgggacca
    10321 gccgcatcgg ttcgccagcg gatcgattct agctgggcgc tgatgccgat cgtggcggcg
    10381 tccggcttcg cgggccttgg ctacgagatc gtgtggaccc gccagttgag cctcgcgctc
    10441 ggcagcgaga tgatggcggt gctgggcgca gttgctgggt tcttcgccgg gctggcgctt
    10501 ggagcctttg tcctcgaccg cccgatccgg cgcgccaggt caccgcgaac ggcgtacgtg
    10561 ttactggaag ctgtcatcgg gctctggggc ttgatctgcg tctggctgct gcccgtcgct
    10621 ggccgagcct tgtcgcccct gctcggtacc gagcctgcgc cggccctgct ttgggcggcc
    10681 agctttgcac tgccgacgtt ggtgctgtta cccgcaaccg cggcaatggg cggaacgttg
    10741 acagcgcttg aacggattgt gcgggaggcg cgcggtgatc cccgcgtcag cgccggcata
    10801 tacggtgcaa acactctcgg cgccgtcgca ggcacattga tttcgacttt cctgctgttg
    10861 cccgcatggg ggctttccgg cacgcttatt tgtcttgccg gagtgaacgc cgtgtgcgcc
    10921 ctcgggatgc ttgcgctcgg atcggcggct ggtcggaagt cggtggaagc caccgaagag
    10981 gtggtccaga ttggcgggcc acgcctgaca attaccttgt tcgcaaccgg cctgctcgga
    11041 attgcgttcg agatccttgt cgtccgactc gcagcgcagg tgatgcaaga cacgatctac
    11101 accttcgcgg ggttgctggc ggcctatctg ctcggcacag cggccggagg ccttgcatgg
    11161 cagcgggccg gacgccgggt tcaggatgcg agtctgggag ggctgctcgc agggaccgcg
    11221 ctggcctgtc tgacgacagc gttcctcacg ccctatatcg ctcgcatcgc ggagaccgcc
    11281 gtggagggag gtctcgctgg agaactggtt gtcgcgatgg cgctgtttct tgtgccgtcg
    11341 gccgccatgg gggccctgtt cggccttctg gcgcagcgga cacgcgatca gcggggctca
    11401 ctgggatggg cggtcggcat caacagtgtc ggcgcctcgg tggcgccgct gctgacggcg
    11461 caggtcgtca ttcccaccct tggcgcatgg accgcaatca ttccggtagc gctgggttac
    11521 ctgctcttga tcccgctccg tcgcgcggca tttgtctggg ccgcggcacc ggcgctcgtc
    11581 gccgttgcgc tgtgggtacg ccctgctccc tctctgacaa gggtgcctcc gggtggcgcg
    11641 ctgctagcgg tccgtgaggg gccaatggtg acggcgagcg ttgtcgatga cgctttcggg
    11701 gcgcgatatc tggaggtcaa cggccgcttc cgcatgggcg gtacaagttc ggtgcgatcg
    11761 gaccatcggc aggcaatgct gccgctgttg ctacatccag caccgcatcg ggcattgttt
    11821 ctgggcatcg gaaccggggc caccctggtc ggtggatcgc agatgccggg cgtgaacgtg
    11881 cacggcgtcg aactatcgcg cgaggtggtc gagttgttgc cctggttcgc cgatccgggg
    11941 gcgcccgccg cggcaccacg ggtgaccgtc gccgacgcgc gccggtttat cgccgcggat
    12001 accacccaat acgatgtgat catcgccgac ttgtttcatc cggcactgga cggcagcggc
    12061 gctctctata ccaccgacca tttcgagaca gtgcgaagac gattggctca aggcggcgtg
    12121 ttttgccagt ggctgcccct gtatcagctt gatctgccat cgttgcgcgc gatcgtccgc
    12181 ggatttctgg agatctaccc cagcggttca gcctggctca accattatag cgttcgcacg
    12241 ccgatgctcg ccttgatcgg ccctcgcgat ggcggcaccc tcgatctgga cgcgctggac
    12301 acgcggcttc gcgatccggc cgtcaggcct gtcgcgcggc ggttgggctt tgagacatcg
    12361 atggatttgc tcgggcagta tcttggaggc gcgcgtacgc tgtccgcctt tgccggccaa
    12421 gggccgcgca acaccgacga ctatcctttt gtaactttcg atgcgcgccg caatgtgcag
    12481 gcgctgacgg ctccgccatg ggccctgctg cgcgccgtca cccaggcgtt gcggcccgat
    12541 ccggcggagc tcctggcaga gccaacgcgc gatgcgctga gcgcgcgcct tgcggcctat
    12601 tggcgcgcgc gc
//
LOCUS       DATNOI010000427        16555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2511261,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000427 DATNOI010000000
VERSION     DATNOI010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 16555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16555
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2511261"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..2151)
                     /locus_tag="VN968_17235"
     CDS             complement(178..2151)
                     /locus_tag="VN968_17235"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL31013.1"
                     /translation="MRQAEDIRQALPIEAVAANPDHVLDLRKVCKQFGTDPAVDALID
                     VDLVLRRGEWLSITGPSGSGKSTLLNILGCLDRPTRGKYLLDGIETTTLSENERAGLR
                     SRRIGFIFQSFHLLPYRTVLENVMLAEVYRRHTGRGRRERAMEEIRRVGLAHRADFPP
                     SKLSGGECQRVAIARALMGSPSLLLCDEPTGNLDSKNTESILDFFTELNKEGMTIVVV
                     THDENVARRGSRRVNMKDGALFGGSEPALAASSRAVITASGITARDLLAEAIAGVIAR
                     PARMALTVLGIVIGMSALVATIGLTRTAGNRIISQFDQLAATELFISARPGIATGTID
                     PRAIPWDAPERLARLNGVVAAGLLSDVNVHDTLVSASPVVDPVNQTALRLAVRAASPD
                     LFRAVRAELGSGRFLDGGHSTRADRVAILGPDAARRLGISGVERLPAIYVGDQLYLVI
                     GILRDVVRKPELLGSVIIPEGTARRHFSLFGPGTVVVETKIGAASLIALQARAALRPD
                     DPRALRVQLPQEPRRVRDDVQTDLNVMFLLLGGLSLVVGALGIANITLVGVMERTAEI
                     GLRRAIGATRRHIAAQFLIESASMGVVGGLLGSSVGVLIVVGVSAYQVWTPVLDPLAP
                     LLAPAVGGVIGLLSGVYPASRAAALEPAEAFRH"
     gene            complement(2148..3884)
                     /locus_tag="VN968_17240"
     CDS             complement(2148..3884)
                     /locus_tag="VN968_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HXL31014.1"
                     /translation="MVTKRVNVLWAILGVVGGMAVGAWYMGSRIQSPAEMAARTAAPE
                     ASAILVPVESRVLSSDVVTRGTVRFGLPQPISIAPSTVKGGIGLISSLPRPNTGFGEG
                     EVILSASGRPVFVLRGETPAFRDMAPGTSGGDVRQLEEALARLGFDPGTVDGNYDQKT
                     SAAVERWYQKAGWDPFGPTREQRAAILALERDWSDAARGRLAAEATHETAVRAVAAAR
                     AIAEQNTRQAAVDSAARAGDSRQLADARAGKSLALETERARATHSATAASADVATQIA
                     DRALIVLDPRQTETARAAAEAKLKVARAAQRKAKLEADLAIQTAAREASLAEERIKVA
                     EAAVKAARLEGERSVRAALELQTLAEFDVKVASERAERLDRELAAARARIGVQVPADE
                     VVFIPSLPIRVHEVTASVAANASGPVMSVTDNQLSIDSQLQIEAAPLVKPGMKAAIDE
                     QALGIKATGIVETVANTPGTRGVDGYHFYLGVRVESTPVLLAGFSVRLTIPIETSKGA
                     VIAVPTSAVSLAADGTSRVLVDRGGRQEYVTVQPGLSTGGYVEVSTPDGRLAPGQLVV
                     VGYKAAESTAVK"
     gene            complement(4034..4903)
                     /locus_tag="VN968_17245"
     CDS             complement(4034..4903)
                     /locus_tag="VN968_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31015.1"
                     /translation="MRKAICAGLFAVLLAPVMAAAQAPKGLGTEEFGMSPRELVQAIE
                     KTEELIARCMADQGFQYIAADHNTVRAGMSADKSLPGLSEEEFVSRYGFGVSTFYTGL
                     PPQLSTGYNPARVGLGDRNVQIFRKLSSADQVAYNRALLGDDLSSTFAVALETEDLSR
                     TGGCTRKSLTETFKPDQLKASHYNPQDALINKDPRMKAAIESWQREMKKAGFEYGHPD
                     EIEPDLRNRLSALTEGGKLLVAKMSADQKAALRKLQDFERLVAARSFKMSEDVLKPVE
                     EKIQEELFSRKVQ"
     gene            complement(4951..5247)
                     /locus_tag="VN968_17250"
     CDS             complement(4951..5247)
                     /locus_tag="VN968_17250"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HXL31016.1"
                     /translation="MKGTIHACLLVIAFLAPAMAAAQTQQTVPPAERAPAGAKITKEQ
                     ATKVALEHMPGKVTDVTIERKRGKNVYVVEIQSLDQGEKDVFVDMESGKVVGTD"
     gene            complement(5390..6088)
                     /locus_tag="VN968_17255"
     CDS             complement(5390..6088)
                     /locus_tag="VN968_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31017.1"
                     /translation="MNTMHRHNCWIAAIGALFAMAYPLAGAAAEDGRFEKLSVYLERN
                     VQDHDAEIRFDVTGATDGLAALKVMAPGERTVIDLRSPDSKLGVRGLTLESPEPDDDK
                     IVRADFPAGAYRFEGTTTKGAHLRGEAQLSHVFPEPAAFEYPRPDQKDVPATALTLRW
                     SVPKGIEACAVVIEQTGSAYEIRALLPGSARSFTVPEGFLRAGKAYKFAIGTVAKDGN
                     RSFIEGSFTTARAR"
     gene            complement(6171..7034)
                     /locus_tag="VN968_17260"
     CDS             complement(6171..7034)
                     /locus_tag="VN968_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31018.1"
                     /translation="MAVLTIFICFGLSSGGVMAQKGKVAEDTLEAFDHNNFDNSANVD
                     NKWFPLQPGTQLVYKGFTQEDEKRVPARVVQTVTDLTKVVNGVRTVVVWDVDSKDGKV
                     TESEIVFFAQDKDSNVWLLGELVEIYEEGDFKGGSAWIAGMDGSSPGIMMKAGPRPGT
                     PSYSQGYAPPPFNWTDRATVEKAGQKVCVPAGCYKDVLVIVEGSKGEGPDAQQLKYYA
                     PGVGYIKVGWRGKGEKLRETLELAEIVKLTPDALAKARAEALDIEKRAYLYGRTPPAE
                     LMKGAANAKAE"
     gene            complement(7105..10278)
                     /locus_tag="VN968_17265"
     CDS             complement(7105..10278)
                     /locus_tag="VN968_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXL31019.1"
                     /translation="MEECFLMMRLIIGSSLRFRYLVLALGAVLTWFGLARLRDVPVDV
                     FPEFAPPRVEIQTICLGLSSAEVEQLVTVPLENALNGVPDIDVMRSKSVAQLSSILLI
                     FKTSADEIRARQLVSERMALATKTLPTWAAPPFMMPPLSSTSRIMKIGITSKDKSVMD
                     LSMLAYWTIRQRLLGVPGVANIAIWGEQLKMLQVMVDPKRLAEADVTLDQVQEAVSDA
                     LDVGLLRYARGAHIGTGGFVETPNQRFQLRFVGGGVTPETLAKVPVTKLIGKPPLLLS
                     DVANLVYAPQGMIGDAVINDGPGLMLIVEKFPWGNTLAVTRGVEEALEKMKAGLPGIE
                     IDTTIFRPATFVEDSIHNLSWALLLSCLLVAGIILIFLYEWRTAIVCIVAIPLSLLAA
                     GLVLFKSGASVNTMTLAGFVIALGIVVDDAILDVENIMRRLRLARRGGDTRSTARIIL
                     DASLEMRAPIVYATLIVVTAVVPVLFMQGLTGSFFKPLITAYMLAIAASLAAAMTVTP
                     ALCLILLRGARLEGRESPVIVWLLRHYAPLLERVTRTPRMAYIAVAVVTLSGIGVWPL
                     LGHSLLPSFKERDFLMHWVTTPDASLPEMLRITTRASKELRAIPGVRNFGAHIGQAFA
                     ADEVVGVNFGENWISISRDADYDKTHAKIEEMVEGYPGLYRDVQTYLKERIREVLTGA
                     GEAIVVRIFGPDLDILRDKTEEVRAALADVPNLVNLHKELMVEVPHIQVTVKLEEAQR
                     YGLKPGDVRRASATLMAGEEVGDIFLGGRTYDVQVWTSPDSRHSVDSVREMLINTPTG
                     QRVKLAEVADVSIRPTPNVVKREASSRRIDVQANVKGRDLGAVASDVQARLDKLAFPL
                     GYYAVLQGEYQELSAARRRLELYSLLALAGVFVLLQQSFNSWRLAALSFLTLPSALVG
                     SVLAAWLAGGVISLGSLVGFLTVLGIAARNGIIMINHFQHLERHEGLSFGMGLVLRGA
                     GERLRPILMTTGAAGLAILPLIIFGDLPGHEIEYPMAVVILGGLVTSTLLNLFILPAF
                     YLRFGRGTGALTDSLTVPGAVRVSGSA"
     gene            complement(10319..10750)
                     /locus_tag="VN968_17270"
     CDS             complement(10319..10750)
                     /locus_tag="VN968_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31020.1"
                     /translation="MARFNVLVVAASTACLAVSCGSALAAESAVKSAPARVEATLDAK
                     IMKVTLTPKAAERLGVVIDEVRVDPSGRRIVPYGSVLYDLTGKTWVYISADPLTFFRG
                     AVEIDTIKGDNVYLSDGPPTGTKVLAAGVPQVYGAEVKVGH"
     gene            complement(10798..13923)
                     /locus_tag="VN968_17275"
     CDS             complement(10798..13923)
                     /locus_tag="VN968_17275"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXL31021.1"
                     /translation="MLAWLISSSMRLRTLVVVVAAGLLVLGAVDVRNKPLDVIPELAL
                     PSLTVKTESLGLSSAEVESLITVPLEADLLNGVPWLQVIQSESMAGLSTIEMIFEPGT
                     DLMKARQMVQERLTQSRALPNVSSPPTMLQPVSSASRVMNIGLSSKSVSLIDMSVQAR
                     WNITPRLAGVPGVANVSIWGQRERQVQVLVDARNLNKKGVKLDQVIKTTGEAVWSSPL
                     TYLNSSTPGTGGFIETPNQRLSIRHQLPISTVGTFAKVPLSGTTTSVGDVAYLVEGHQ
                     ALIGDAIVGENPGLMVAIEKFPGFNTLDVTRGVERALNELKPGLPGIEINTTIYHPAS
                     FIERATSNLGKAMLISIILAAVVLGLLMGSWRTAVIPLAAIALSFVCAELLLRAAGIP
                     LSMIVVGGLLMASGVIVHDSIIDVENVLHRLRAPQRDGRSGFMVVVRAMLETRRPMLY
                     ASLIVVLAVLPVLFMYSRSAAFFAPMAWAYIAAVSISMLVALIVTPALAAVLLANAPL
                     TPTGGWVINRLARPFDSIVGRAIHAPLACIGACIVAAIACFALWSPLERNMVPSFRET
                     DLVIEWQGPPGTSLQAMTRSTENLIRDLRQIPGVQNAFANLGRAVLCNCERAMDVNAG
                     QVWVNIDPKADREETVDAVEAAVTAYPGMRGKLGTYLSTKLREALTGDPDSITVRVYG
                     NSLDVIRAKAEEIRASMAKIPGIEDPRVELQVEEPSIEVKVDVDRAAGYGLKPGDVRR
                     ATSAMVGGITVGALFEDQKVFDVVVWGRPELRDNIDDVRNLMINSESGSQVRLAQVAD
                     VRIAPATSIIQRQGSSRRIDVSAKVDERALGDVADDVARNVKQIAFPFEHRAEVLGEY
                     KEQRAALRSIYGYMAAAAVLMFLLTQAVLRSWALTALSLLGVPVAVLGGLATAAINDG
                     HFSLGSLLGLAAVLALMVRQGIGLVAHFQHLQLNEGEPFGEKLVRRGVHEQFPSVIAS
                     SVTTLALVLPFAIMGDVAGLEIAHPLALAVLGSIVTMTLGTLFVLPALYLRFGAGWTA
                     DRLDLEMETAS"
     gene            14483..16303
                     /locus_tag="VN968_17280"
     CDS             14483..16303
                     /locus_tag="VN968_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31022.1"
                     /translation="MISGKRDEFVAGSRRRRHAAKIATVELTERGWHRICAVYPLIFR
                     AAPALFSLVLLVPSYAAAAENEDMAVLRRMLGELKAENRKLSQRLSALEGGSAARHTK
                     PAAAHESPAPAAAAAIPAPAVGSPPVALPAPDLSEAGTKRPLDQRVKDLEIGWAAQEN
                     ATQQIIRDTLSKTGPKINNYLALSGVVEGLASRTGQFNGPTQENLALSTAELDFDIKL
                     SDWLTGALVLHFDNGTGAIFPTGNNPVVPTTPGVGVDRFTLDRTHISIGDLMQFPVAA
                     RFGVETLHFGTSTGVARLDTLSIGTPLTTEVFENRQTAGGLEFAWPTPPRQPPPAPVV
                     IPRVSPLVVAPAVSQLARWLGYTPLPERPFRPQPVTFPFDPPPFYGSVMTYKGSDSII
                     PNRTKIDDYNASLGFRTRGHCGVPYDQLTNSPICPWTLDFHVDYDTSVFESRFLHASY
                     LPFLSQIGQIPGVAASVKASFGPFAFVGEVNAALQQARFLDGLGTARSMTPMAWQASI
                     AYQFDWNPWITEIGAQGDFISLGYSGSKDMAGVTGLVNGLPTRIGFVPQNRVFLTAGE
                     WVMDGLKVAVEYSANWDYPVSSGGTGKVAHGGFGLVQLNF"
BASE COUNT         3146 a         5308 c         4988 g         3113 t
ORIGIN      
        1 gcggccctcg aagactacca aggctccatt ttgctacctg ggatcaaaat cagcactgga
       61 ccttggaact ttcggccgat caaacaccta cgactggttc aattcgacgg ccgcacgtgg
      121 caaccgattg gcgaagtgct cgaaacggct ttctccaacg cccaaaagta gcatgctcta
      181 gtgtcggaac gcttcggccg gctcgagcgc ggcagcgcgc gaggcagggt agactccgga
      241 cagcagaccg atcacgccgc cgacggctgg cgcgagcaag ggagcgagcg ggtcgagcac
      301 cggagtccag acctgatacg ccgagacacc caccacgatc agcactccga cgctggatcc
      361 aagcagtccc cccacgacac ccatggacgc actctcaata agaaactgtg ctgcgatgtg
      421 gcggcgagtc gcgccaatgg cacggcgcag tccgatctcc gccgtccgct ccatcacgcc
      481 caccagggtg atatttgcga tcccaagcgc gccgaccacg agcgacaggc cgcctagcag
      541 caggaacatg acattgaggt cggtttgcac gtcatcgcgt acgcgccgcg gctcctgcgg
      601 cagttgcacg cgaagcgcgc gtggatcgtc cggccgcagc gccgcccgcg cctgaagcgc
      661 gatcagggaa gcggcgccga tcttcgtctc cacgacaacc gtgccaggtc cgaacaggct
      721 gaaatgccgt cgcgccgtac cctcgggaat gattaccgaa ccaagcagtt caggctttcg
      781 caccacatcg cggaggatac cgatcacgag gtagagttga tcgccgacat aaatcgcggg
      841 cagccgctcg actccactga tgcccaggcg gcgcgcggca tcggggccga ggatcgcgac
      901 gcggtcggcg cgggtagaat gcccgccgtc gagaaaccgt cccgagccga gttcggcgcg
      961 caccgcgcgg aacaaatccg gcgaggcggc gcgcaccgcc agcctgagcg ctgtctgatt
     1021 gaccggatcc accacgggtg aggcgctgac gagcgtgtcg tggacattga cgtcgctgag
     1081 caagcccgcg gccaccacac cgttcagccg ggcgagccgt tccggcgcgt cccacggaat
     1141 ggcacgcgga tcaatcgtgc cggtcgcgat tcccggccgc gccgagataa aaagttccgt
     1201 ggctgcgagt tgatcgaact gactgatgat gcggttgccg gcagttcgag tgaggccgat
     1261 ggtggccacc aacgccgaca tgccgattac gatgccaagc accgtcagcg ccatgcgggc
     1321 cggccgcgcg atcaccccgg cgattgcctc ggcgagaagg tcgcgcgctg tgatccccga
     1381 agccgtaatg accgccctgg atgaagctgc caatgccggc tcgcttccgc caaagagcgc
     1441 gccatccttc atgttgaccc ggcgcgagcc ccggcgcgcc acgttttcat cgtgcgtgac
     1501 gacgacaatc gtcattcctt ctttgttgag ttcggtgaag aagtccagga tcgactcggt
     1561 gtttttggaa tcaagattgc cggtcggttc gtcgcacagc aacaaactcg gtgagcccat
     1621 caaggcccgg gcgatcgcca cccgctggca ttcgccgcct gacaatttgg acggcggaaa
     1681 atccgcgcga tgcgcaaggc cgacgcggcg aatttcctcc atcgcccgtt cgcgccgccc
     1741 gcgtccggtg tgccggcggt atacttccgc cagcatgacg ttctcaagga cggtacgata
     1801 cggtaggagg tgaaatgact gaaagatgaa tccgatgcgc cggctgcgca atccggcccg
     1861 ctcattctcg ctcagcgtcg tcgtttcgat accatcaaga aggtactttc cgcgggttgg
     1921 ccggtcgagg caacccagga tgttcaacag cgtcgatttc cccgagccgg acggaccggt
     1981 tatcgacagc cattcgccgc gccgcaacac caggtcgaca tcgattaatg cgtcgaccgc
     2041 gggatcggtc ccgaattgct tgcacacctt acgcaggtca agcacgtgat cgggattggc
     2101 ggcaaccgct tcgatcggca acgcctgccg tatgtcttcg gcctgcctca tttcaccgct
     2161 gtcgactccg ccgctttgta gcccaccacc acgagttggc cgggggcgag tcgcccatcg
     2221 ggggtgctca cctcgacata gccccccgtg gaaagccccg gttgtacggt gacgtattcc
     2281 tgtctgccgc cgcgatcgac cagcacgcgc gatgttccgt cggcagccag tgacaccgcg
     2341 ctcgtcggga ccgcgatgac cgcgcccttc gaggtttcga tcgggattgt cagccggacg
     2401 gaaaaccctg ccaggagcac aggcgtgctc tctaccctga ccccgaggta gaagtggtac
     2461 ccgtccacgc cgcgcgtgcc gggtgtgttc gccaccgtct caacgatgcc agtcgccttg
     2521 attccaagag cctgctcgtc gatcgcggcc ttcatcccgg gcttcaccag cggcgcggct
     2581 tcaatttgca gctgcgagtc gatactgagc tggttgtccg tgacactcat caccggcccg
     2641 gaagcgttgg cggcgacgga agcggtcacc tcgtggacgc ggatcgggag cgacgggatg
     2701 aacacgacct cgtccgccgg aacctgcacg ccgatcctcg cgcgcgccgc cgcgagttcg
     2761 cggtccagtc gctcggctcg ttcgcttgcg accttgacgt cgaattcggc gagcgtttgc
     2821 agttccagcg ccgcacgcac gctgcgctcg ccttccaggc gggcggcctt tacggctgcc
     2881 tcggcgacct tgattcgttc ctcggccaga ctggcctcgc gcgccgcggt ctgaatcgcc
     2941 agatcggctt ccagcttcgc cttgcgttgc gcggcacgtg ccactttcag cttggcctcg
     3001 gccgcggcac gcgccgtttc ggtctggcgc ggatcgagaa cgatcagggc ccgatcggcg
     3061 atctgcgtgg cgacatcggc gctggccgcg gtcgcggaat gcgtggctcg cgcgcgttcg
     3121 gtttcaagcg cgagggattt gccggcgcgc gcgtctgcta gctgacggct gtcgccagca
     3181 cgcgccgcgc tgtcgacagc ggcctggcgc gtgttctgct ccgcgatggc ccgcgcggcg
     3241 gcgaccgctc gcaccgctgt ttcgtgcgtg gcctcggccg caagtcggcc gcgcgctgca
     3301 tccgaccagt ctcgctcgag cgcgaggatg gcggcgcgtt gttcgcgcgt cggtccgaac
     3361 ggatcccacc ccgccttttg ataccagcgc tcgaccgcgg cggatgtctt ttgatcatag
     3421 ttgccatcga ctgttcccgg atcgaagccg agacgggcca gagcttcttc gagctggcgt
     3481 acgtcgccgc cgctcgttcc gggcgccatg tcgcgaaaag ccggtgtctc gccgcgcagg
     3541 acgaagaccg gacggccgga tgctgacaga attacctcgc cttccccaaa tcccgtattt
     3601 ggtcgcggca gcgacgagat cagtccgatg ccgcctttga ccgtggaagg cgcgatcgaa
     3661 atcggctgcg gcagcccgaa gcggaccgtg ccgcgggtga cgacatcgga actcaataca
     3721 cgcgactcga caggcacgag gatcgcggat gcctcgggcg ctgcggtgcg cgccgccatt
     3781 tccgccggcg actggatcct ggagcccatg taccaggcgc caaccgccat gccgccaacc
     3841 acgcccagaa tggcccagag aacatttacg cgttttgtga ccatgccgta atccttccgg
     3901 ccattgacag cgcgcaggcc atcttcgcga gaagcgttgc gtcggccttc gtcacgcgat
     3961 gtccggccgc gaaaggaggt tactcatcgc cgccgatccg tggaagtacg gaaaactgat
     4021 gtcatgatcg atctcattgc accttccggg aaaataattc ctcttggatc ttctcctcga
     4081 ccggtttcaa cacgtcctcc gacattttga agctcctggc cgcgaccaga cgctcgaaat
     4141 cctgcagttt ccgcaacgcg gctttctggt ccgccgacat cttggcaacc agcagcttgc
     4201 cgccctcggt cagcgcgctg agccggttcc gcaggtcagg ttcgatttcg tccgggtggc
     4261 cgtattcgaa gccggccttc ttcatctcgc gctgccaaga ctcaatcgcg gccttcatgc
     4321 gcgggtcctt gttgatcagc gcgtcttggg gattgtagtg gctggccttc agctggtcgg
     4381 gcttgaacgt ttcagtgaga gacttgcgcg tgcagccacc ggtccgcgat aaatcttcgg
     4441 tctccagggc gaccgcaaag gtgctgctca ggtcatctcc gagcaacgcg cgattgtaag
     4501 cgacctgatc tgcggaggaa agtttacgga aaatttgcac attgcggtcg ccgagaccaa
     4561 cacgggcggg gttgtagccg gtcgatagct gaggcggcaa acccgtatag aaggttgata
     4621 cgccaaatcc ataacgactg acgaactcct cctcgctgag gcccgggagc gatttgtctg
     4681 ccgacatgcc cgcccgcacc gtgttgtggt cagccgcgat gtactggaaa ccctgatcgg
     4741 ccatgcagcg cgcgatcagt tcctcggtct tctcgattgc ctgaacaagc tcgcgcggcg
     4801 acatgccgaa ctcctcggtg ccaaggccct ttggcgcctg tgccgcggcc atcaccggcg
     4861 caagtaggac ggcgaacaaa cccgcgcaga tcgcttttct catgacgcta tcctctccaa
     4921 tcacgaacct cgtcaggacg gcatcccgtc tcagtcggtg ccgacaacct ttccggactc
     4981 catgtcgacg aagacgtcct tctcgccctg atccagcgac tggatctcga caacgtagac
     5041 attcttgccg cgctttcgtt caatcgttac gtcggtgacc ttgccgggca tgtgttcaag
     5101 cgcgaccttc gtcgcctgct cttttgtgat ctttgccccg gccggcgcac gctcagcggg
     5161 aggaactgtc tgctgcgtct gcgcggcggc catcgccggc gctaggaaag cgatgacgag
     5221 caggcacgcg tggatcgttc ccttcatggc aatatccttt cctgaaaaga aaccacatag
     5281 agttggcatt ccgccttcgc cgcgcgcgtg tccacgaaca cgcgaactcc tccccagatc
     5341 gcgcggcctc ggcgcgtaac ctcggaacat cggcttggtc gagatcgatt catcgcgccc
     5401 tcgccgttgt gaagcttcct tcgatgaagg accggttgcc gtccttcgca acggtcccga
     5461 tcgcgaattt gtaggccttg ccggcgcgca agaacccttc aggcaccgtg aaggatcttg
     5521 ccgagcccgg cagcagtgcc cggatttcgt aggccgagcc ggtttgctcg atcacgacgg
     5581 cgcaggcctc gatgcccttt ggcaccgacc accgcagcgt gagcgcggtg gcgggtacgt
     5641 ccttctggtc cggccgcgga tattcgaacg cggccggttc ggggaatacg tggctcaact
     5701 gcgcctcacc gcgcaggtgc gcccccttgg tcgtcgttcc ctcaaaacga taggcgcccg
     5761 ccgggaaatc ggcgcgcacg atcttgtcgt catcgggctc gggcgattcg agcgtgaggc
     5821 cgcgaactcc caacttcgag tccggcgatc taaggtcgat caccgtgcgc tcgcccggcg
     5881 ccattacctt cagcgccgcg agcccatccg tggcaccggt tacatcgaac cggatctcgg
     5941 catcgtggtc ttgcacgtta cgctcgagat agacggagag cttttcgaag cgaccatcct
     6001 cggccgccgc tcccgcgagc ggataggcca tggcgaacaa agcgccgatg gcggcaatcc
     6061 agcagttgtg gcgatgcatg gtgttcacat cagcctccca cagatacccc taccggttct
     6121 ggaccagtcg gacgtttcgc cggcacgctg ctgcagggcc ggcggaatac tcactcggct
     6181 ttagcatttg ccgcaccctt catcagttcg gcgggcggcg tacgaccata gaggtaggcg
     6241 cgcttctcta tgtccagcgc ctcggcgcgg gccttggcca gtgcatcggg ggtgagcttc
     6301 acgatctcgg ccagctcaag ggtttctcgc agcttttcgc cctttcccct ccaaccgact
     6361 ttgatgtagc ccacaccggg tgcgtagtac ttgagctgct gggcgtccgg gccttcaccc
     6421 ttgctgccct ccacgatcac cagaacatcc ttgtagcagc cggccgggac acagaccttc
     6481 tgacctgctt tttctaccgt tgcgcgatca gtccagttga acggaggcgg cgcataaccc
     6541 tgagagtagc tgggggtccc cggccgcggg cctgccttca tcatgattcc cggactcgag
     6601 ccatccatgc cggcgatcca ggcgctaccg cccttgaagt ccccctcttc gtatatttca
     6661 accagctcac ccagcagcca gacgttgctg tccttgtctt gcgcgaagaa aacgatctcc
     6721 gattccgtca ccttgccgtc cttggagtcg acatcccaca caacgaccgt acgcacgccg
     6781 ttgacgacct tggtgaggtc ggtcacggtc tgcaccacgc gagcaggaac gcgcttctcg
     6841 tcctcctggg tgaagccctt gtagaccaac tgagtccctg gctgcagcgg gaaccatttg
     6901 ttgtcgacgt tggcggagtt atcgaaatta ttgtgatcga acgcttcgag cgtgtcctcg
     6961 gcaaccttgc ccttctgcgc cataacgccg ccgctggaca gtccgaagca aatgaaaatc
     7021 gtaagcacgg ccaaggccgt tattctctga cacaatctga tggccgcttg tgccatcctg
     7081 gactgctccc aacttacgtt catttcacgc actcccagag actcgtaccg ctcctggaac
     7141 agtcaggctg tccgtcagcg cgcccgtacc gcgaccgaaa cggagataga atgcaggcaa
     7201 gataaacagg ttgagcagcg tcgatgtcac gaggccgccc agaatgacga ccgccatcgg
     7261 atattcgatc tcgtggcccg gcaggtcgcc gaagatgatc agtggcagga tcgcgagccc
     7321 ggcggcgccg gtcgtcatca ggatcgggcg caaccgctcg cccgcgccgc gaagcacgag
     7381 ccccattccg aacgacagtc cttcatggcg ctccaggtgc tggaagtgat tgatcatgat
     7441 gattccattg cgagctgcga tgccgagcac ggtcaggaac ccgaccagcg agccaaggga
     7501 aatcacaccg cccgcgagcc acgccgccag cacgctgccg accagcgccg aaggcagggt
     7561 caggaagctg agcgctgcca gacgccagct gttgaaggat tgttgcagaa gtacgaagac
     7621 gccggcgagc gccaggaggg agtacagttc gagccgcctg cgggcggcgc tcagttcctg
     7681 atattcgccc tgcagcaccg cgtagtagcc aagaggaaac gccaacttgt ccagacgcgc
     7741 ctgcacgtcg ctggcgaccg cgccgagatc acggcccttg acattcgcct gcacgtcgat
     7801 ccgacgggag ctcgcctcac gcttgacgac atttggcgtc ggccgaatgc tgacatcggc
     7861 tacttccgcc agcttgacgc gctgcccggt cggcgtgttg atcagcatct cgcggaccga
     7921 gtccacgctg tggcgggagt ccggtgacgt ccacacctgc acgtcgtagg tgcgcccgcc
     7981 gaggaagatg tcgccgacct cctctccggc catcaaggtc gcggaggcac gccggacatc
     8041 gcctggcttc agaccgtagc gttgtgcttc ctccagcttc accgtcacct gaatgtgcgg
     8101 tacttcgacc atcagctcct tgtgcaggtt gacgagattc ggaacgtccg cgagtgccgc
     8161 gcggacctcc tccgtcttgt ccctgagaat gtcgagatca gggccgaaga tgcggacgac
     8221 gatcgcctcg ccggcgccgg tcaggacctc gcgaatgcgc tccttcagat aggtctgcac
     8281 atcccggtag aggccgggat agccctccac catttcctcg attttggcat gggtcttgtc
     8341 gtagtcggcg tccctgctga tgctgatcca gttctccccg aaattgacgc cgacgacctc
     8401 atcggcggcg aaggcctggc cgatgtgggc gccgaagttg cgtacacccg gaatggcgcg
     8461 cagttctttg cttgcacgag tcgtgatacg cagcatttcg ggcagcgacg cgtcgggggt
     8521 ggtgacccag tgcatcagga agtcgcgctc cttgaacgac ggaagcagcg agtggccaag
     8581 cagcggccag acaccgatcc cagagagcgt cacgactgcg acagcgatat aggccatgcg
     8641 aggtgtccgt gtgacccgct ccagcaaggg cgcgtaatgg cgcagcagcc agacgattac
     8701 cggtgattca cgcccttcga gtcgcgcgcc acgcagcagg atcagacaga gcgccggcgt
     8761 taccgtcatc gccgcggcga gtgaggccgc aatcgcgagc atataggctg taatcagtgg
     8821 cttgaagaag gatccggtca ggccttgcat gaacaggaca ggcacgaccg cggtaaccac
     8881 gataagggtt gcgtagacga tcggcgcccg catctcgagc gaagcgtcca gaatgatgcg
     8941 cgccgtgctt cgcgtgtcgc cccctcgccg cgcaagacgc aggcgccgca tgatgttctc
     9001 gacgtcgagg atcgcgtcgt cgacgacgat ccccaacgcg atcacgaagc ccgccagggt
     9061 cattgtgttg accgaggcgc cggacttgaa caaaaccagt ccagctgcga gcagcgacag
     9121 cggaatggcg acgatgcaga cgatcgcggt acgccattcg taaaggaaga tgagtatgat
     9181 tccagcgacc agcagacagc tcagcaacag cgcccatgac aggttgtgga tcgagtcttc
     9241 gacgaaagtt gccggccgga agatggtcgt atcgatttca atccctggca ggccggcctt
     9301 catcttctcc agcgcctcct ccaccccacg ggtgacggcg agcgtgttgc cccaggggaa
     9361 tttctcgacg atgagcatga ggcctgggcc gtcgttgatg acggcatcgc caatcatgcc
     9421 ctgcggtgca tagacgagat tggccacgtc gctcagcaac agcggcggtt tcccgatcag
     9481 tttggtgacc ggtaccttgg ccagcgtctc aggtgtaacg ccgccgccga cgaagcggag
     9541 ctgaaagcgc tgattaggtg tttcgacgaa gccgccggta ccgatatgcg cgccgcgggc
     9601 atagcgcagc agaccaacat cgagggcatc ggaaaccgct tcctgcacct ggtcgagcgt
     9661 aacgtccgcc tcggccaagc gcttcgggtc aaccatgacc tgaagcatct tgagctgctc
     9721 tccccagatc gcgatgttcg caacgccggg aactccgagc agccgctgcc ggatagtcca
     9781 ataggcgagc atcgacaggt ccatcacgga cttgtccttg gaggtaatgc cgatcttcat
     9841 gatccggctg gtggaggaca atggcggcat catgaacggc ggcgcggccc atgtcggcag
     9901 ggtcttggtc gcgagcgcca tccgctcact gaccagttgc cgcgcgcgga tctcgtcggc
     9961 gctggtcttg aagatcaaca ggatcgacga caattgcgcc accgacttcg aacgcatcac
    10021 gtcgatgtcc gggacaccgt tcagcgcgtt ttccagcggc actgtgacaa gttgttcaac
    10081 ttcggcggat gaaagcccaa gacagatggt ctggatctcg acccgcggcg gcgcgaattc
    10141 cggaaagacg tccacgggca cgtcgcgcag gcgtgcgagg ccaaaccatg tcagcaccgc
    10201 gccaagggcg aggacgagat aacgaaagcg cagactagaa cctatgatca gcctcatcat
    10261 cagaaaacat tcctccatcc aggccgaagc cgttgtggaa aaacaattcg acgcgcagct
    10321 agtgacctac cttcacctcg gccccgtaga cttgtggcac tccggccgcg agcaccttcg
    10381 tgccggtagg tggcccgtca ctcaggtaaa cgttatcgcc cttgatagtg tcgatctcca
    10441 ccgcgccacg gaagaaggtc aacggatcgg cgctgatata gacccaggtc tttccggtca
    10501 ggtcgtagag aacggatcca tacggcacga tccggcggcc cgacggatcc acgcgaacct
    10561 catcgatcac gacgccgagc cgttcagccg ctttcggcgt gagcgtcacc ttcatgatct
    10621 ttgcgtccag cgtggcctcg actcgcgcag gcgcgctctt gaccgccgac tccgcggcca
    10681 gggctgagcc acaagacacc gcgaggcacg cggtagacgc cgcgaccacc aatacgttga
    10741 agcgggccat gagacgttcc tccctttcga cggctttcga agtgacgcgc gcggcagtca
    10801 tgacgcggtc tccatttcaa gatcgagccg gtcggccgtc cagccggcac cgaaccgcaa
    10861 gtaaagcgcc ggcagcacga acaatgttcc cagcgtcatg gtaacgatgc tgcccaggac
    10921 cgcgagcgcg agcgggtgcg cgatttcgag tccggcgaca tcgcccatga tcgcgaacgg
    10981 aagtaccaga gcgagcgtcg tgaccgatga tgcgatgacg ctcgggaatt gctcgtggac
    11041 gccgcgccgc accagctttt cgccaaacgg ctcaccctcg ttcaattgca gatgctggaa
    11101 atgggcgacc agaccgatac cctgccgcac catcagtgcg agcaccgctg ccagacccag
    11161 cagagaacca agcgagaagt gaccgtcatt gatcgcggcc gtagcgaggc cgccgagtac
    11221 agcgaccggt acaccgagca gcgacagcgc ggtgagtgcc cagctgcgga gcaccgcctg
    11281 cgtcagcagg aacatcaaca ccgccgccgc cgccatgtag ccgtagattg accgcagggc
    11341 agcccgctgt tccttgtatt cgccgagcac ctcggcacgg tgctcgaacg ggaacgcgat
    11401 ctgtttgaca ttcctggcga catcatcggc cacatcacca agcgcccgct cgtccacttt
    11461 cgcggaaaca tcgatacgac gcgaggaccc ctgtcgctgg atgatgctgg tggccggcgc
    11521 gatgcggacg tccgcaactt gagccaggcg aacctgggac ccgctctcgc tgttaatcat
    11581 cagattccgc acgtcatcga tgttgtcgcg caattccggt cgcccccaga cgaccacgtc
    11641 gaaaaccttc tggtcctcga aaagcgcacc gacggtgatg ccgccgacca tcgcggaagt
    11701 cgccctgcgg acgtcgcccg gcttaaggcc gtaaccggcc gcgcgatcga catccacctt
    11761 gacttcgatc gagggctctt cgacctgtaa ttcgacccgg ggatcttcaa tgccggggat
    11821 cttcgccatc gatgcgcgga tctcctccgc cttggcgcga atgacatcga ggctgttgcc
    11881 atagacgcgc acggtgatgg aatccgggtc accggtgagc gcctcgcgca gcttcgtgga
    11941 caggtatgtc ccaagcttgc cgcgcatccc gggataggcg gtgacggccg cttcgacggc
    12001 atccaccgtt tcttcgcgat cggccttcgg gtcgatgttg acccacactt gcccggcatt
    12061 cacgtccatt gcgcgttcgc aattgcaaag caccgcccgt ccgagattgg caaaggcgtt
    12121 ctgcactccc gggatctgcc gcaggtcgcg gatcaggttc tcggtcgatc gggtcattgc
    12181 ctgcagcgag gtgccgggcg gtccctgcca ttcgatcacg agatccgtct ctctgaagct
    12241 gggcaccata ttgcgttcga gcgggctcca gagcgcgaag cacgcgatcg ccgccacgat
    12301 gcaggcgccg atgcaggcga gcggcgcgtg gatggcgcga ccgacaatcg aatcaaaagg
    12361 cctggccaat ctattgatga cccaaccgcc cgtcggggtc agcggcgcat tggcgagcag
    12421 caccgcggcc agcgcggggg tcacgatcaa cgcaaccagc atcgagatgg acactgcggc
    12481 gatataggcc caggccatcg gcgcgaagaa cgcggcggac cggctgtaca tgaacagaac
    12541 gggcagcact gcgagtacga cgatcagcga ggcgtacagc atcggccgac gcgtttccag
    12601 catcgcacgg acgacgacca tgaagccgga ccgaccgtcg cgttgcggcg cacgcaagcg
    12661 gtgcagcaca ttttcgacgt cgataatgct gtcatgcacg atcacgccgg acgccatcag
    12721 caggccgccg acgactatca tgctgagcgg gattccggcg gcccttaaca gcagttcagc
    12781 acagacgaac gacaatgcga tcgcggccag ggggataacc gcggtcctcc aactgcccat
    12841 cagaaggcca agcacgacgg ctgccaagat gatcgaaatg agcatggcct tgcccagatt
    12901 gctggtcgcg cgttcgatga agctcgcagg atggtagatg gtcgtattga tctcgatacc
    12961 gggtagcccg ggtttcagtt cgttgagcgc gcgctcaacg ccgcgcgtca cgtccaatgt
    13021 gttgaagccg gggaatttct cgatcgccac catcaggccg gggttctcgc cgacgatggc
    13081 gtctccgatc agcgcctgat gcccctccac gagataggcg acgtcgccga cgctcgtggt
    13141 cgtgccgctg agcggcacct tggcgaaggt acccacggtg gagatcggca gctggtgacg
    13201 gatgctgagc cgctggttcg gcgtttcgat gaagccgccg gtgccgggcg tggatgagtt
    13261 caggtaggtg agcggcgagg accacaccgc ctcacctgtc gtcttgatca cctggtcgag
    13321 cttgacgccc ttcttgttca ggttccgggc atcgacaagc acctgcacct gccgctcgcg
    13381 ctgaccccat atcgatacat tggccacacc cggaactccg gcgaggcgcg gcgtgatatt
    13441 ccagcgcgcc tgaacggaca tgtcgatcag tgagacggat ttcgagctca agccgatatt
    13501 catgactcgg ctggccgagg aaaccggctg aagcatggtc ggagggcttg agacgttcgg
    13561 aagtgcacgc gactgcgtca gtcgctcctg caccatctgg cgcgctttca tcagatcggt
    13621 gccgggctcg aagatcattt cgatcgtcga aagtcccgcc atcgattccg actggataac
    13681 ctgaagccac gggacgccat taagcaggtc cgcttccagc ggcacggtga tcaacgattc
    13741 gacctcggcg ctggagaggc caagcgattc cgtcttcacg gtgagcgagg gcagcgccag
    13801 ttcggggatg acatcgagcg gcttgtttct gacgtcgacg gcgccgagga ccagcaggcc
    13861 cgcggcgaca accacaacaa gcgtgcgcaa ccgcatgctc gaggaaatga gccacgccaa
    13921 catctcgacg ccccccctct tttgacctcc aacccaagtc ctaagagccc cgttgaacac
    13981 gggattttct ggccgctatc tcactcgtag ggcaacgcag ttatttccct acgagtcaga
    14041 accttttcag ttgcacctcc gccgtatcgc ctcagcactt cggtctcgcg aacgccatgg
    14101 tgagcatcgc ggtgatttag cggagaccga agactgagca ttctagacca aatcagggca
    14161 atacggatat tttgatgttg agcttttggc gacacaatgt cgcaccttaa ggtagcacaa
    14221 ctaatcgatt actgctcccc cttaattcgg atacatcact tcgtggcgct tgagtaattc
    14281 gccgtcagtg tcattgttgt gcaacgttgt tgaggggctt ccggcaattg ttgctccgtc
    14341 ttgttgagcg ggctcttgtc tgcaatcgac ttgacggatg aaacgctggc tgagagagtc
    14401 tggttacgcg agcgaaattg ttacgagcac gttagttcgg ctgtttgagt gacgtccgac
    14461 tacggtggtg atgaggccgc gcatgatctc gggtaagcgg gatgagttcg ttgccggatc
    14521 caggcgacgt cggcatgcgg caaaaattgc aacggtggag ttaacggagc gcggttggca
    14581 tcgcatctgc gcggtgtacc cattgatctt tcgcgctgcg ccggcattgt tcagcctcgt
    14641 cctgcttgtc ccttcttatg ccgccgccgc ggagaacgag gacatggcag ttctccggcg
    14701 catgctcggg gagctaaagg cggagaaccg caaactttcc cagcgtctca gtgcgctgga
    14761 gggcgggtcg gcggctcggc ataccaagcc cgctgcggcg cacgagagtc cggcgcctgc
    14821 cgcagccgcg gcgatacccg ctccggcggt tggctcgccg cccgtcgctc tgcccgctcc
    14881 agatctgtcc gaagccggga cgaaaaggcc gcttgaccag cgtgtgaaag accttgagat
    14941 cggctgggcg gcgcaggaga acgcgacgca gcagatcatc cgggacacgc tttcaaagac
    15001 cgggccgaag atcaataact acctcgcgct tagcggtgtg gttgaagggc tggcctcgcg
    15061 gaccggccaa ttcaacggcc ctacccagga aaacctggct ctgagcacgg cggaactcga
    15121 tttcgatatc aaattgagcg actggttgac cggtgctctg gttctccact tcgacaatgg
    15181 cactggcgcg atattcccga ccggaaacaa tccagttgtg cctaccaccc ctggcgtagg
    15241 cgtggatcgc ttcacgcttg atcggactca catttcaata ggcgatttga tgcaattccc
    15301 ggtcgctgcg cgattcgggg tggagacttt gcatttcggt acgtcaacgg gtgtggcacg
    15361 cttggatacg ctctcgatcg gcactccgct gacgaccgag gtgttcgaaa accgtcagac
    15421 ggcgggaggc ttggaattcg cctggccaac cccaccgcgg caaccgccgc cagcgcctgt
    15481 cgttattcca cgagttagtc cgctggtcgt cgcaccggcc gtgagccaac tcgcgcgttg
    15541 gctcggctac actccgttgc ctgagcgtcc gttccggcct cagccggtca cattcccctt
    15601 cgacccgccc cccttctacg gctcagtcat gacctacaag ggcagcgata gcatcatccc
    15661 caaccgcacc aagatcgacg attacaatgc cagtctcggt ttccggacca ggggccattg
    15721 cggcgtgccg tatgaccagc tgacgaactc gccgatttgt ccatggaccc ttgacttcca
    15781 tgtcgactat gacacctccg ttttcgaaag cagatttctg catgcgtcct acctgccatt
    15841 cctgagtcag atcggccaga ttccgggagt ggccgcgagc gtcaaagcca gcttcggacc
    15901 attcgcgttc gtgggcgaag taaatgccgc gctccagcaa gccaggtttt tggatggtct
    15961 cggcaccgcc aggagcatga cgccgatggc ctggcaggct tcgattgctt accagttcga
    16021 ttggaacccg tggataacgg agatcggcgc ccagggcgac ttcatctcgt tgggatattc
    16081 aggcagcaaa gacatggcgg gcgtgacagg cctggtcaac ggcttgccaa cccggatcgg
    16141 attcgtcccg caaaaccggg ttttcctcac cgcaggcgag tgggtgatgg acgggcttaa
    16201 agtggccgta gaatattcag ccaactggga ctatccagtg agcagcggcg gtaccggcaa
    16261 ggtcgctcat ggtggtttcg gactggtcca actcaatttt tgatccgtct ttgcctagag
    16321 cgggatgact tttcttcgaa tcgtcatccc gctctatctt ttgtttgagc atgatctttt
    16381 cggaaaaccg gtgcccactt ttccggatca tgctctagcg ctttaaggac gcgtgtgccg
    16441 aaatcctaga agcagtcgtc acgacggatg gagcgagcag accgcttcca ccctgcccca
    16501 caccagcacg caggaagatc gatgactccc gcgttagcgg caaatgcccg ccgtt
//
LOCUS       DATNOI010000428         4311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2616127,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000428 DATNOI010000000
VERSION     DATNOI010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2616127"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..463
                     /locus_tag="VN968_17285"
     CDS             110..463
                     /locus_tag="VN968_17285"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HXL31023.1"
                     /translation="MLRFWRATINTRNGLAFAIRSEQAVREELAALALSLPMAWLVGA
                     TTMRRVELIAVVVLVLVVELLNTAIEKLADRLTTAHDPQIGRVKDMGSAAVGVALVMA
                     GLFWLFALAERMGAF"
     gene            546..2303
                     /locus_tag="VN968_17290"
     CDS             546..2303
                     /locus_tag="VN968_17290"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HXL31024.1"
                     /translation="MTERAAQFSITLAQLNPTVGDVEGNAAKARVAREQARADGADLV
                     VLPELFITGYPPEDLVLKPAFQSACRAAVEELARDTADGGPAMLIGSPWVEDGKLYNA
                     CALLDEGRIAAIRFKANLPNYGVFDEKRLFARGPAAGPVTVRGIRVGVPICEDIWLEE
                     SEDYENVVECLAETGAEILVVPNGSPYARDKNDLRLSIAVARVTESGLPLIYLNQVGG
                     QDELVFDGASFALNADLSLAAQFPAFAENITTLRWTKGEAGWRCTGPMAPVVEGDKGD
                     YAACVLGLRDYVRKNGFPGVLLGVSGGIDSALCAAIAVDALGADHVRGVMLPFRFTAQ
                     VSLDDAAKLARALGIRYEVLPIAQAVNGFEEILSGTFAGLPRDITEENLQARTRGTLL
                     MAISNKTGAMVVTTGNKSEMSVGYATLYGDMNGGYNPIKDIYKTEVFRLSALRNSWQP
                     DGALGPSGEVIPVNIITRPPTAELRENQTDQDSLPPYDMLDAILERLVEREEPLASIV
                     AAGFDRAVVTRIDRLLNIAEYKRRQAAPGVKVTEKNFGRDRRYPITNKFRDSGKALPE
                     PDETLVSRASRGSAEAFDG"
     gene            complement(2380..3531)
                     /locus_tag="VN968_17295"
     CDS             complement(2380..3531)
                     /locus_tag="VN968_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HXL31025.1"
                     /translation="MPETLITLFSRWILACAALFGAVALSVDASAQINPGPPPSQGAA
                     VNPMCPRLEAQLATIDRGGGGDPAKDEQIRRYQDAAAKQQSELDRVTSQAKRMGCDSS
                     GFFSLFSGQSAQCGPVNNQIQQMRANLEQITTSLERLRGGFGGSERDSQRRSVLAALA
                     QNNCGPQYANAARGPGSFLETLFGNNNNNNNPMTPGVDLGPQSGTYRTVCVRTCDGFY
                     FPISFATVPARFPDDEKTCKNLCPAAEATLFSYRNPGEDMNQAVSINGQSYSSSPNAF
                     RYRQEFNPSCACKAAGQTWSDALKTIDDKASAEQQGDIIVTEESAKKMSRAPTKGAAA
                     AAKKGAAPAGTTATAPAAPDASAPAANSTAAAPDNKPIRSVGPTFIPAR"
     gene            complement(3684..3992)
                     /locus_tag="VN968_17300"
     CDS             complement(3684..3992)
                     /locus_tag="VN968_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31026.1"
                     /translation="MRSLPAFLALMFVLAASAASADSRVFIVANQADGYGVDQCLAMG
                     EKCGAHAAQSYCQSRDFAQALSYRRVDPDEVAGSIPATAAANCNHAGCNDYVAITCQR
                     "
BASE COUNT          751 a         1314 c         1450 g          796 t
ORIGIN      
        1 cgcctcaacg ccgagcagtc gatcgacatg gccttcctga tcgcggaatt gctcaagcag
       61 gaacgcggcg gcaaggtcca gccgatgccg gtcgcggcgg gactttgagt tgctgcggtt
      121 ctggcgggcc acgatcaata cgcgcaacgg cctcgccttt gccatccgct cggagcaggc
      181 ggttcgcgag gagctggcgg cgctggcgct ctcgctgcca atggcctggc tggtcggggc
      241 gaccacgatg cgccgtgtcg agctgatcgc cgtcgtcgtt ctggtgctgg tggtcgaatt
      301 gctcaacacc gcgatcgaga agctcgccga ccggctgacc accgctcatg atccgcagat
      361 cgggcgggtc aaggacatgg gctcggccgc ggtgggcgtt gcgctggtca tggccggcct
      421 gttctggctg ttcgccctcg ccgaacgcat gggcgcgttc taaagcatgc aattggcatc
      481 tggcgaagcc cgagagggca tgctatattt catttgctca ttatgaggat aagtaggttc
      541 gctccatgac cgaacgtgcc gcccagtttt cgatcacgct ggcgcagttg aacccgacgg
      601 taggcgacgt cgagggcaac gccgccaagg cgcgcgtcgc gcgcgaacag gcgcgggccg
      661 acggcgccga tctcgtggtg ctgcccgaat tgttcatcac cggctatccg ccggaagacc
      721 tggtgctgaa gccggcgttt cagtcggcgt gccgtgccgc ggtcgaggaa ctggcgcgag
      781 acaccgccga cggcggtccg gccatgctga tcggcagtcc ctgggtcgag gacggcaagc
      841 tctacaatgc ctgcgccttg ctcgacgagg gccggatcgc ggccatccgc ttcaaggcca
      901 acctgccgaa ttatggcgtc ttcgatgaaa agcgcctgtt cgcgcgcggt cccgccgcag
      961 gccccgtgac cgtccgcggc atccgggtcg gggtgccgat ttgcgaggat atctggctcg
     1021 aagagtcgga ggattacgaa aacgtcgtcg aatgtctggc ggagaccggc gcggaaattc
     1081 ttgtggtgcc gaacggctca ccctatgcgc gcgacaagaa cgatctgcgg ctgtcgatcg
     1141 cggtggcgcg cgtcaccgaa agcgggttgc ccctgattta tctcaatcag gtcggcggac
     1201 aggacgaact ggtgttcgac ggcgcatcgt ttgcgttgaa tgccgatctc tcgctggcgg
     1261 cgcaatttcc ggctttcgca gaaaacatca cgacgctgcg ctggaccaag ggcgaagccg
     1321 gctggcgctg caccgggccg atggcgccgg tggtcgaggg cgacaagggc gactatgccg
     1381 cctgtgtgct gggcttgcgc gactatgttc ggaaaaacgg atttcccggc gtgctgctgg
     1441 gcgtatccgg cggcatcgac tcggcgctat gcgcggcgat tgcggtcgat gcgctgggcg
     1501 ccgaccatgt ccgcggcgtc atgctgccgt tccgtttcac ggcccaggtg tcgctcgatg
     1561 acgcggcaaa gctcgctcgc gcgctcggca tccgctacga ggtgctgccg atcgcgcagg
     1621 cggtgaatgg ttttgaagag atcctgtcgg gcacgttcgc aggcttgccg cgcgatatca
     1681 ccgaagagaa cctgcaggca cgcacccgcg gcacgctgtt gatggcgatt tccaacaaga
     1741 ccggcgcgat ggtggtcacg accggcaaca aatcggaaat gtcggtagga tacgccacgc
     1801 tctatggcga catgaatggc ggctacaatc cgatcaagga tatctacaag accgaggtgt
     1861 tccggctctc ggccttgcgc aattcatggc agccggacgg cgcgctgggc ccctcgggtg
     1921 aagtcattcc ggtgaacatc atcacccggc cgccgaccgc ggaactgcgc gaaaaccaga
     1981 ccgatcaaga ttcgctgccg ccttacgaca tgctcgatgc gatcctggaa cgactggtcg
     2041 aacgtgagga gccgctggca tcgattgtgg ctgcggggtt cgatcgcgcg gtggtgacgc
     2101 ggatcgatcg cctgctcaac atcgccgagt acaagcggcg ccaggccgcc ccgggcgtca
     2161 aggtcaccga gaagaatttc ggccgcgacc gccgctatcc catcaccaac aagtttcgcg
     2221 attccggcaa ggcgctgccg gaacccgacg aaacgctggt ttcccgcgcc agccgcgggt
     2281 cggcggaagc tttcgacggc taaggtctga ttcgcaaaaa ccggttccga cccatcgggt
     2341 cgtcgcccga cggcgtgctt ttccggatca tgcactggat cagcgcgcgg ggataaaggt
     2401 cgggcccacc gaacggatcg gcttgttgtc cggcgcggcg gcggttgagt tggccgcggg
     2461 ggccgatgcg tccggcgctg ccggcgcggt ggccgtggtg cctgccggcg cagccccctt
     2521 cttggcggcg gctgcagccc ccttggtcgg cgcccgcgac atcttcttgg cgctctcctc
     2581 ggtaacgatg atgtcgcctt gctgttcggc ggaggctttg tcgtcgatgg tcttcagggc
     2641 gtccgaccac gtctgtccgg cagccttgca cgcacaggag ggattgaact cctgacggta
     2701 gcgaaacgca ttcggggacg acgaatagga ctggccgttg atcgacaccg cctgattcat
     2761 gtcctcgccg ggattgcgat agctgaagag ggtggcctcc gccgccgggc acagattctt
     2821 gcaggtcttt tcgtcgtcgg gaaaccgggc gggaaccgtg gcgaacgaga tcggaaaata
     2881 gaacccgtcg caggtgcgga cgcagaccgt gcggtaggtc ccggattgcg gacccagatc
     2941 gacgccgggc gtcatcggat tgttattgtt gttgttattg ccgaacagag tttccaggaa
     3001 actgcccggg ccgcgcgcgg cgttggcata ttgcggcccg caattgttct gcgccagcgc
     3061 ggcgaggacc gagcggcgct gactgtcgcg ctcagagccg ccgaagccgc cacgcagtcg
     3121 ctcgaggctg gtggtaattt gctccagatt agcgcgcatc tgctgaatct ggttgttgac
     3181 cggaccgcat tgcgccgact ggccgctgaa cagcgagaaa aatccggaac tgtcgcagcc
     3241 catgcgcttg gcctgcgagg taacgcggtc gagctcgctt tgctgcttgg ccgccgcatc
     3301 ctgatagcgg cggatttgct cgtccttggc cgggtcgccg ccacccccgc ggtcgatggt
     3361 cgccaattgc gcttccagcc gcgggcacat cgggttgacc gcggccccct gcgacggcgg
     3421 cggccccgga ttgatctgcg ccgaggcgtc aacgctcagc gcgaccgcgc cgaacagcgc
     3481 agcgcaggcc aaaatccatc tggaaaagag ggtgatcagc gtttcaggca tatccggcca
     3541 ttagagcaac tccgcgcagg tttgaaccga aaggcgcggc caacgcgggg cctgacatag
     3601 ccgcttctcg gggcaccgtc acgtcgtttc cggggcgccg gtgtggcaga ggcggctttc
     3661 gacttggccg ccggggggcg ctcctagcgc tggcaggtaa tagccacgta gtcgttgcag
     3721 ccggcatggt tgcagtttgc cgcggcagta gcgggaattg acccggctac ttcatccggg
     3781 tcgacgcgcc ggtaggacaa ggcctgggcg aaatcccgcg actggcaata ggactgggcg
     3841 gcgtgggcgc cgcatttttc gcccatggcg aggcactggt cgacgccgta gccgtccgcc
     3901 tggttggcga cgatgaagac ccggctgtcg gccgatgccg ccgaggcggc aaggacgaac
     3961 atgagggcca gaaacgcggg gagggatcgc atgctgcacc aaaaaaaaat gaggggaccg
     4021 cccctcattc gatagactca aaatgttaac aatgattaac catggcggcc gggacggatt
     4081 gatggccgaa atcggttttt tgacggctct tgacgccgtc acccgagctg gcccatggta
     4141 tttggcatga acggttctct cgccatctgt ggcatttgcc gcgagattat gagctagcga
     4201 ttcgctggct gaggccgtct tttctcacac gagatcactg gacgcccggc cgcaagggac
     4261 gggatatcca tggacctgcg actctacgat accttgacca aggaaaagcg c
//
LOCUS       DATNOI010000429         4164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_575661,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000429 DATNOI010000000
VERSION     DATNOI010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575661"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VN968_17305"
     CDS             complement(<1..455)
                     /locus_tag="VN968_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXL31027.1"
                     /translation="MRAILDYCTGGIQRSVSAGTLILHEGGTTGHLYVLIEGRLEVVK
                     GDTVVAIITEPGAVLGEMSVLLDQPHTATVRAASDSAVYEIGDAASFLRDQPAVALLI
                     ARLLAQRLNVATTYLADIMQQYAGHGDHLAMVGEILQTMINLPPITISPG"
     gene            complement(631..828)
                     /locus_tag="VN968_17310"
     CDS             complement(631..828)
                     /locus_tag="VN968_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31028.1"
                     /translation="MFPTEIVDGQWGSRGVIRPLPDIQAFIAGEHKRKVGEERLARRR
                     RVKALAMLEAALDAARKGAGT"
     gene            complement(829..1155)
                     /locus_tag="VN968_17315"
     CDS             complement(829..1155)
                     /locus_tag="VN968_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31029.1"
                     /translation="MIDALIPEGALKPEAEARLLKELTDILITHEGFEPGNKVAQSVS
                     VVFLHRPAAVFVGGTRSASPRYRIIPTVPEGQYNDASREALVRDVTEAVVRADGGKYE
                     DVAPRV"
     gene            complement(1335..3275)
                     /locus_tag="VN968_17320"
     CDS             complement(1335..3275)
                     /locus_tag="VN968_17320"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HXL31030.1"
                     /translation="MSDFFRAHKAVGTKIPTYFIESTAPENVDDTVVASSKLPPELTG
                     KLTLIDSDLAVQEIAKALASSADPNLVVMVHGFNNPEPAVLRMYTSAAMTIQGDNAIC
                     SRQGLVCVGYRWPSENMGKPWRGTWDALPTLPTWILYFGVAVVVLTFPLFYFASETTQ
                     WWIDLFRYIWNPNGVHVITLIGWTVAGLVLMTVLLRTIVYFRDNYRATNYGVPDLVQV
                     IRAIDSEIARLRGDGPSTNDVQLSFIGHSMGGFVVTNTIRVLSDAFAREVSALNSYGA
                     DAAPEPTSRPIGKVFSLMRFVLASPDIPAETLLSSRGNFLASALSRFEEAYLFSNEGD
                     EVLRQISTLANYFVFPTKSRNHGFRLGNVEILSRNFGLIDVKEDDFLRVLRIGNMTLQ
                     ELYDALEDAKAERQVNAGPVPEQAALPKVFTYYDCTDYVDQVDGDKQARPLLSFAKWR
                     KRNDPEAKLRWYSHLYLLLAYVIRGQKPNVHGGYFEGLLSQQLIYRLACLGYSGTVAA
                     FGDEVALGKACEQKQIRMLVSPRLWASRVSSSAPPPPVQPTMRPMSRTIAPAPPAPAI
                     GVVPNLVGRSIVEARAAVAEARARHVGPVALPVRGSEPQLLLLEFTRESPGNPPGTVL
                     SQTPAAGSPLQQRTSIEVVVAK"
     gene            complement(3381..4160)
                     /locus_tag="VN968_17325"
     CDS             complement(3381..4160)
                     /locus_tag="VN968_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL31031.1"
                     /translation="MDRLFAQGTVSAAHSGEGPPLFLLHSLLSDRASFDAIVPELAKS
                     FRVLVPELPGFGRSQAVTGGLAAVAGRMAEAVRDAAAGEQAIVLGNGYGGFVALQMAI
                     RHPDIAAKFILADCGAAFSEAGREAFRNMAAASNAKGLAAITDVAMRRLFAPQFQADH
                     PELMRDRREAFLRTDPDVFRAACDALAGLDLRPELGRVEVPVLVLVGEHDEATPPPMS
                     HELAAGLPHARLEIIAGCAHVPQLQSPEMFLEAIGDFLPVS"
BASE COUNT          832 a         1336 c         1259 g          737 t
ORIGIN      
        1 cctggcgaga tcgttatcgg cggcaggttg atcatggtct gcagaatctc gccgaccatc
       61 gcgaggtgat cgccgtggcc ggcatattgc tgcatgatgt cggcaagata ggtggtcgcg
      121 acattgagcc tctgggccag cagccgcgcg atcagcagcg ccaccgccgg ttggtcgcgc
      181 agaaacgatg cggcatcgcc gatctcgtag acggccgaat cggatgccgc gcgcacggtc
      241 gcggtatgcg gctgatcgag cagtaccgac atttcgccca gcacggcgcc gggctcggtg
      301 ataatggcga cgacggtatc gcctttgacg acttcaagcc gtccctcgat caggacgtag
      361 aggtgtccgg tggtgccgcc ctcgtggagg atgagcgttc ccgccgaaac gctgcgttga
      421 attcccccgg tgcaatagtc caaaatcgcg cgcattcagc gacactccca agcccttgac
      481 tccaagccct ggggcaagat taggcagcag ggttgcgacc gtcgaacgga taatgtggtc
      541 gatctgctca aattgtttgc atctaacaga ggccaggtgg tgccgtcaga gaccacgagg
      601 cgaaagcgcg aggcgagtca cgcgatgggg ctacgtgccg gcacctttgc gcgcggcgtc
      661 gagagccgct tccagcatcg ccagcgcctt cacgcgacgg cgccttgcga gccgttcttc
      721 accgaccttg cgcttgtgct cgccggcgat gaatgcctgg atgtcaggaa gcggccggat
      781 cacgccgcgg ctgccccact gcccatcgac aatctcggtg ggaaacactc agacacgggg
      841 agcgacgtcc tcatattttc cgccatccgc ccggaccacc gcttcggtca cgtccctcac
      901 cagcgcctcg cgtgatgcat cattgtactg cccctccggc accgtcggga tgatccgata
      961 gcgcggcgat gccgaccgcg tgccgccgac gaacaccgcc gccggccgat gcagaaacac
     1021 gacggacacg ctctgtgcca ccttgttgcc gggctcgaac ccctcatgcg tgatcaggat
     1081 atcggtgagc tccttgagca gccgcgcttc ggcttccggc ttcaaggcac cctcgggaat
     1141 tagcgcatcg atcatcggca tggcagaagg cgccaatagc ccggccgacg tgctgctgac
     1201 ggtcgatatc ggccggcggc ttcgacgctg gtggacaagg tcggtttggc cgggaggcgc
     1261 aaatcgcgac aagtcgtggc tggatggtct cttatgcgtc tcaagttgcc gcataccagg
     1321 cccggccccg cccgttactt ggcgacgaca acctcgatcg acgtgcgctg ctgcaacggg
     1381 ctgccagccg ccggggtctg gctcagcacc gttccgggcg gattgcccgg cgattccctt
     1441 gtaaattcga gcagcaacaa ctgcggttcg gagccgcgga ccggcagggc caccggcccg
     1501 acatgacgag cgcgggcctc agcgacggcc gcgcgggcct cgacgatcga cctgccgacc
     1561 aggttcggaa ccacgccgat cgcaggtgcc ggcggtgccg gcgcgatcgt ccgtgacatc
     1621 ggccgcatcg tcggctgaac cggcggaggc ggggcggacg aactgacccg gctcgcccac
     1681 agccttggcg acaccagcat gcgaatctgc ttttgctcgc acgcttttcc cagcgcaacc
     1741 tcgtcgccga aagccgccac ggtaccgcta tagccaagac aggcgagccg gtagatcaat
     1801 tgctgcgaca ataatccctc gaagtagccg ccatgcacat tcggcttctg tccgcgaata
     1861 acgtaagcga gaagcagata caaatgcgaa taccagcgca gcttcgcttc agggtcattg
     1921 cgtttgcgcc atttcgcgaa gctgagcagc gggcgcgcct gcttatcgcc atcgacctga
     1981 tcgacatagt cggtgcaatc gtaataggta aacactttgg gtaacgcggc ctgctccggt
     2041 accggccccg cattcacttg ccgctcggcc ttggcgtcct caagcgcatc gtaaagctcc
     2101 tgcagcgtca tatttccgat ccgcaggaca cgaagaaagt cgtcctcctt cacgtcgatc
     2161 aatccgaaat tgcgcgacag gatttcgaca ttgccgagcc ggaagccgtg attgcggctc
     2221 ttggtcggga acacgaaata gttcgcaagc gtcgaaatct ggcgcaggac ctcgtcgcct
     2281 tcgttgctga acagataggc ctcctcgaaa cgcgacagcg ccgacgccag aaaattcccg
     2341 cggctcgaca acagcgtctc ggccggaatg tccggcgaag ccagcacaaa gcgcatcagg
     2401 ctaaagactt tgccgatcgg gcgcgacgtc ggttccggcg cagcatccgc gccatatgag
     2461 ttcaacgcgc tcacctctct ggcgaacgca tccgaaagga cgcgaatcgt attggtaaca
     2521 acgaacccac ccatgctgtg cccgatgaag gaaagctgga cgtcgttggt cgaagggccg
     2581 tcgccacgca acctggcgat ttcgctgtcg atggctcgga taacctggac caggtccgga
     2641 acgccataat tggtggctcg gtaattgtcg cggaaataaa caatggtacg cagcagcacg
     2701 gtcatcagca ccagaccggc cacggtccag ccgatcagcg tgatcacgtg gacgccgttg
     2761 ggattccaga tgtagcgaaa cagatctatc caccattgcg tggtttccga cgcaaaatag
     2821 aacagcggaa acgtcaacac gacaacagca acgccgaaat acaaaatcca cgttggcagc
     2881 gtgggcaagg catcccaggt gccgcgccac ggcttcccca tattctccga cggccagcgg
     2941 taaccgacgc agaccagacc ttgccggctg caaattgcgt tgtcgccctg gatggtcatc
     3001 gcagcggacg tgtacattcg caacacggcc ggctccggat tattgaagcc atgaaccatc
     3061 accaccagat tgggatccgc gctactcgcc agggctttgg cgatctcctg caccgccaga
     3121 tcggaatcga tcagtgttag cttccctgtt agctccggcg gcagtttcga cgatgccaca
     3181 acggtgtcgt cgacattctc gggcgccgtg ctttcgatga agtaggtcgg tatcttcgtc
     3241 ccgacggctt tatgagctcg gaagaaatcc gacatgatcc actcccctgc tgccagcctt
     3301 cttgtctctg gctaggctag ggagatatca ggacattgac aagcttgggt tgcaaatatc
     3361 ctgcggtccg cgaatgtgat ttatgacacc ggcaggaaat cgccgatcgc ctccagaaac
     3421 atctcgggcg attgcagctg cgggacgtgc gcgcagcccg caatgatttc aagacgcgcg
     3481 tgcggcaagc cggcagccag ttcgtgcgac atcggtggcg gcgtcgcctc gtcatgttcg
     3541 ccgaccagaa cgagaacggg gacctcgacc cgcccgagct cgggccgcag gtcaagcccg
     3601 gccagcgcat cgcaggcagc gcggaacacg tccgggtcgg tcctcaggaa tgcttcgcga
     3661 cgatcgcgca tcagttcggg atgatccgcc tgaaattgcg gcgcgaacag ccggcgcatc
     3721 gcgacgtcgg tgatcgcggc gagccccttg gcgttggatg ccgccgccat gttgcgaaaa
     3781 gcctcgcgcc cggcttccga aaacgccgcg ccgcaatcgg caagaataaa cttggccgcg
     3841 atatcagggt ggcggatcgc catttgcagc gcgacgaagc cgccataacc gttgccgagc
     3901 acgatcgctt gctcgcctgc cgcggcgtcc ctcaccgcct ccgccatgcg gccggcgacg
     3961 gcggcaaggc cgccggtcac ggcctgcgag cggccaaaac ccggcaattc cggaacgagc
     4021 acgcgaaagg attttgccaa ttccggcacg atggcgtcga aactcgcgcg gtccgacagc
     4081 agcgagtgaa gcaggaacag cggcggtccc tcgcccgaat gtgcggcgct gacggttcct
     4141 tgcgcgaata gccgatccat cccc
//
LOCUS       DATNOI010000430         7948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_719564,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000430 DATNOI010000000
VERSION     DATNOI010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719564"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..589
                     /locus_tag="VN968_17330"
     CDS             113..589
                     /locus_tag="VN968_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HXL31032.1"
                     /translation="MAQAETFETDFWKHANLRKLWDDIVPGEPRKTVPYTLTRQAIEL
                     YCKSVGEDHPIYFDEAYAKTTRHGGLIAPPAIHILLMFACTPADDWMRSPGTVNAGQS
                     WSYHVPARPGDVITLQARALDKFIKRERLFVVHDNVFFNQHGEVICSGRGQTIRPT"
     gene            603..1040
                     /locus_tag="VN968_17335"
     CDS             603..1040
                     /locus_tag="VN968_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXL31033.1"
                     /translation="MGTTFENLRTGDAIDGPKFAVSRESIRLFCDASLDYNPLHLDDE
                     YMKGDFGKTNFGGIIMHGMNNFGLISRMITDWAYPRGAIHRRLETRWVKPVRPGDIIQ
                     PTGIVKSKQVTANSRWVVIDVMVRNQLDEKVATGEAMVEFPQG"
     gene            2054..2263
                     /locus_tag="VN968_17340"
     CDS             2054..2263
                     /locus_tag="VN968_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31034.1"
                     /translation="MLNPRVPATISDRQFYQQLAIAGIITEDEALAANAAIIPPPLLD
                     IIDEMPADQQFSIKMIVSGATVFER"
     gene            2683..2787
                     /locus_tag="VN968_17345"
                     /pseudo
     CDS             2683..2787
                     /locus_tag="VN968_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762509.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2974..3684)
                     /locus_tag="VN968_17350"
     CDS             complement(2974..3684)
                     /locus_tag="VN968_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31035.1"
                     /translation="MLSIEGLKVAYGHVEALRGIDMAMKQGEITAIIGSNGAGKTSAL
                     MAISGLAPVTAGDIRFEGQTITRRRPHDITRLGITHILEGRQLFADQTVEDNLLLGGY
                     TRLPRNRVRVAELMEREMTRFPILRERRRQLAGTLSGGEQQMLAIARGLMSEPRVILM
                     DEPSMGLAPLLVKEIASTIRALRKEGATIVLVEQMAATALRLADHAYVLENGRVKLSG
                     TGAELARMPEVKRAYLGG"
     gene            complement(3677..5491)
                     /locus_tag="VN968_17355"
     CDS             complement(3677..5491)
                     /locus_tag="VN968_17355"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HXL31036.1"
                     /translation="MQLPIFAFSGPKDWALVALAALILALLPVVFDDPFFILVMQSLA
                     YLFIVTLGLDILVGWTGQISLGHAGLYAVGAYTTALASARLGLPFWLSAPLGVALAGL
                     FGAALAVPSLRAKGPYLAMVTIAFGFMAEVTSNRWSLTGGPMGIMSIPPPTLPDGSEM
                     TATQYFWLIAGVALLCHLLAANLFRSRMGRTLLALQGSEIAAQSVGVNVYRYKVLAFV
                     ISSVYAGIGGVFFAHQNGFINSDTFVFSLSVSLLTSALMGGSGTLFGPLVGSVILNLI
                     PTVFASLHDYHLYIYGGIILVTIVFLPRGIVGSLQQMPALRRLRRPPAVITPDRSVLD
                     FARDSAQDGPVLVVRGLAKSFGGIRALSGVDVAIKRGTVHGLIGPNGSGKSTFVNVVT
                     GVFRASAGKIMLGERELPPQGPHRMAQHGITRTFQSIRLFVDLSVIDNVMVGFHLQLK
                     SGLWAHLLQTRAAVAEEAAYRGKAMALLELVGIAERAHDPAQNLSYGQQRLLEIARAL
                     AARPQLLMLDEPAAGVNPTELHHLSRIIRDIKAAGVTLLVIEHHMELIMGISDVISVF
                     DFGQKISEGSAAAVREDKKVIEAYLGVAEADHDGAVHA"
     gene            complement(5493..6380)
                     /locus_tag="VN968_17360"
     CDS             complement(5493..6380)
                     /locus_tag="VN968_17360"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL31037.1"
                     /translation="MSLFFAKLWQALVSGLSIGSIYALIAEGYYITFVTTGALNFGQG
                     EFLMIGALFGLTCYVTFGLPYAVAVIAAVLLTGVMGAALERGAIRPVMRHALSLSWVL
                     STVAVSIILKNAAVNLWGPEQIKFPSPFGEAVLQIGSAGIFPQELFIIVAALGTVFAV
                     QMFLRRSLLGKALIAVAFNRNAAAVMGINVPRMVVFAFVLSSALAGLGGILIAPITFA
                     WAYMGTVPGIKAFAAAIFGGLENPIGILIGGLVIGLLEQLLGMVNSNLKEGITFFFIL
                     IVLAIRPTGLMGRREITKV"
     gene            complement(6537..7715)
                     /locus_tag="VN968_17365"
     CDS             complement(6537..7715)
                     /locus_tag="VN968_17365"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31038.1"
                     /translation="MSIEARTTGLRLGAIGIGLLLAAVLGSTAVAADLKIGFTLSLTG
                     GTDDYGKGARMGAELALKEYNDKGGYQSKKVEAVIYDDETKPAKGVENVTRLITRDKV
                     FAMVGPVNSGVALAIIDIAQKNQVPLMDTVATAEQIIERYKNAPKNYIFRVSLNDGIQ
                     TSFMIDYIKGRQYQRVGLMHDSTGWGQSGRDTALRLVKEANLDLVAGPEVFDQNDTDM
                     TTQLTKMQEAKAEFIIAYSLAPAGVQIAKSMQKIGLKTPWTGTWALIAPNFLKLGGKA
                     TVEGVMAVTSYTPDHSANAKVLHEKVDRIFKEQGGDFHPVATAQTYDGVRLLLRALDK
                     VGPDPQKIRDALEEIDDFTEAVTKMKPHPFSHANHESLGRDTGFLAVWHDGKLERASE
                     "
BASE COUNT         1553 a         2489 c         2441 g         1465 t
ORIGIN      
        1 atcgtggttt tcccggcagc ggctcgattg cacagtgtgg caaattgatt ataccatata
       61 acaaaattcg caagacacga aaacagcaag acgtggaaac agggagcata gcatggcgca
      121 ggcggaaacc ttcgagaccg acttctggaa acatgccaat ctgcgaaagc tctgggacga
      181 tatcgtcccc ggcgaaccgc gcaagaccgt tccctatacg ctgaccaggc aagcgatcga
      241 gctctactgc aaatcggtcg gcgaagatca tccgatctat ttcgacgagg cctacgccaa
      301 aaccacccgt catggcggcc tgatcgcgcc gccggcgatc cacatcctgc tgatgtttgc
      361 ctgcacgccg gcggacgact ggatgcgcag cccgggcacc gtcaatgcgg gacaatcctg
      421 gagctaccat gttccggcgc gtcccggcga cgtcatcacg ctgcaagccc gcgcgctcga
      481 caagttcatc aaacgcgagc ggctgttcgt ggtgcacgac aatgtcttct tcaaccagca
      541 tggcgaagtg atctgttccg gccgcggcca gaccattcgg ccgacgtaaa ggggacggga
      601 tcatgggcac cacgttcgaa aatctgcgga ccggcgatgc gatcgacggt ccgaaattcg
      661 cggtcagccg cgaatccata cgcctgttct gcgacgcctc gctcgactac aacccgctgc
      721 atctcgacga cgagtatatg aagggcgatt tcggcaagac caatttcggc ggcatcatca
      781 tgcacggcat gaacaatttc gggctgatct cgcggatgat caccgactgg gcctatccgc
      841 gaggtgccat tcatcgccgc ctcgaaacca ggtgggtcaa gccggtccgg ccgggcgaca
      901 tcatccagcc gaccggcatt gtcaaatcga aacaggtcac ggcgaattcg cgctgggtcg
      961 tgatcgacgt catggtgcgc aaccagcttg acgagaaggt ggcgaccggc gaggccatgg
     1021 tcgagttccc gcagggctga ctgggcggga tttccaatcg tagcgggatc aagtccgggc
     1081 atggcgagta gatttgcact gcgcgaatgc gcgcactcca tttccttctc gtccctgcga
     1141 aagcaggaaa ccatactccg cgcatgcgga gtatccgccg tcattgcgag ccaacgggtc
     1201 gcgcgaatgc gcccgatgac aggctccgcg aagcaatcca tcttgcagcg aaaagaaaga
     1261 gtggaatctt cgtcgcaagt gctcctcgca atgacgttgc tatagtttcg cacacaactt
     1321 cgttctcgcg acgcggagcg cccgaggcgt tgctattacc ttccgcccaa agggcgtggg
     1381 gaatgccgga tgcccgctgc acccgcggcc tcgtgtgcac ttggtattgg tagaaagcac
     1441 acgagtaaca acgagcacac cggaatcacc cggcattccc gcacgcaatg gttttaacgg
     1501 cttccttcgt gctctccccg gtgaccgggc tcttttgcca ccgtcgcttg cggatatggt
     1561 tttgtccaag cccggtaggg ccgacagaac atccgcgaac ttgacgccag cgtcggggcg
     1621 tcaggaccac acgacttcgc cgtacgcaac aacatctctc gtccgcgtgc tcgtcgatcg
     1681 ctcacaagtt tttcaacccg ccctgcgatc ccgttgcgcg caaaacgctg ccgcgtccac
     1741 cgcatcccgc cccgcgtttg tgacgatggc caacgcccct cttgtggggt gggatggcgg
     1801 gagttgtagt agtgatttgg ggggagtggg aatggaaata tttttgcaaa ggggactcga
     1861 cagccctttc aacaaaaggc ccgacgggca aatcaccaga ccgcgcggaa ggtcgaactg
     1921 atggcatatg tcagcatcat caatggaaaa ctgtgtggat tattttctga tccccaaccc
     1981 ggaattgagg gtttggtgga gatagccgga tgatgatccg gcagttgttg ctttcaaagc
     2041 tatgacgaac tcaatgttaa atccacgcgt tccagcgacg atatctgatc gtcaatttta
     2101 tcagcagctc gcaattgccg ggatcatcac cgaggatgaa gcgctcgccg caaatgcagc
     2161 gatcattcct ccgccgcttt tagacatcat cgacgaaatg ccagccgatc agcaattttc
     2221 gatcaagatg atcgtatctg gtgcaaccgt gttcgagcgc taaaatcccc tgaccatttc
     2281 gatcggtacg gcctatggca tgtcttcgga ccagatcgac gccttctttc tggccgcgag
     2341 tctcctccag ctaacgctca cgtccaaggt ggccccatca acccgcgaaa gcgtttctcg
     2401 gcggccgcaa atgctctaaa cttacttccg ctctcaggcc acagcggaca tggccggact
     2461 tgctgctgac tcgatcccat ataggtagca aaatcaatcg tgattacggg tcaacttcgt
     2521 aagtgtcaat tgtactgctt gtccgttgtt gaggggatag tgttgcaaaa gtctttttgc
     2581 gtctctgaac acaaatactt cgggctgtag gcgcggcgat cgtgcgactc tccaccatca
     2641 agacctggcg ggcttcgact tctccttcca gccgtcgcgt ggatctgcct ctcgaacaac
     2701 gccgccgaac gggcggtccg cgcggtggcg ctcggccggg ccaactggat cttcgcgggc
     2761 tcggacgccg gcgtgcagcg ggccgcccgc cgtctagacc gggctatgcc gagtccccga
     2821 ctatgccgaa cccaccgtgt ggtcgatcgg gtggccttgc tcgcacaatt ttcgctgcaa
     2881 agcgcagact cttgcatctc cacagcagcg acgactcagg atcgccgcgc gcatgggcga
     2941 accaccaaca actatgcacg gctacgaact atttcagccc ccaagatacg cccgcttgac
     3001 ctccggcatg cgggcgagct cggcgccggt gccggagagc ttgacgcggc cgttctccag
     3061 cacataggcg tggtcggcga gccgcagcgc ggtggcggcc atctgctcga ccaggacgat
     3121 cgtcgctccc tccttgcgca gcgcgcggat cgtgctcgcg atctccttca ccagcaacgg
     3181 cgcgagcccc atcgacggct cgtccatgag gatgacgcgc ggctccgaca tcaggcctcg
     3241 ggcgatcgcc agcatctgct gctcgccgcc cgaaagcgtg cccgcaagct ggcggcgccg
     3301 ttcacgcagg atcgggaaac gcgtcatctc gcgctccatc agctcggcga cgcgcacgcg
     3361 gttgcgcggc agccgggtgt agccgcctag cagcaggttg tcctcgacgg tctggtcagc
     3421 gaacagttgg cggccttcga gaatgtgggt gatgccgagc cgggtgatat cgtgcggcct
     3481 gcgcctggtg atggtttgtc cttcgaagcg gatgtcgcca gcggtgaccg gtgccagccc
     3541 ggaaatcgcc atcagcgcgg aggtctttcc cgccccgttc gagccgatga tcgccgtgat
     3601 ctcgccttgc ttcatggcca tgtcgatgcc gcgcagcgcc tcgacatggc catacgcgac
     3661 cttgagaccc tcgatgctaa gcatggacag ccccatcgtg gtcagcttcg gcgacgccga
     3721 gataggcctc gatcaccttc ttgtcctcgc gcaccgcggc ggcactgccc tcggaaatct
     3781 tctgcccgaa gtcgaacacc gagattacgt cggaaatccc catgatgagc tccatgtggt
     3841 gctcgatgac gagaagcgtg acgccggccg ccttgatgtc gcggatgatg cgcgagaggt
     3901 gatggagctc ggtcggattg accccggccg ccggctcgtc gagcatcaga agttgcggac
     3961 gcgccgcgag cgcgcgcgcg atctccagca gccgttgttg gccgtaggac aggttctgcg
     4021 ccggatcgtg cgcacgctcg gcgatcccga cgagctccaa gagcgccatc gccttgccgc
     4081 gataggccgc ctcctcggcg acggccgcgc gggtctgcag gagatgcgcc cacaaaccgc
     4141 ttttgagttg caggtggaac ccgaccatga cgttgtcaat gaccgagagg tcgacgaaca
     4201 gtcggatgct ctggaaggtg cgcgtgatgc cgtgctgggc catgcgatgt ggcccctgcg
     4261 gcggcaactc cctctcgccg agcatgatct tgccggcgct cgcccgaaag acgccggtta
     4321 cgacattgac gaaggtgctc ttgccggagc cgtttgggcc gatcaggccg tgcacggtcc
     4381 cgcgcttgat cgcgacatcg acgccgctca acgcgcgaat gccgccgaag ctcttggcca
     4441 atccgcgcac caccaggacc gggccatctt gcgccgagtc gcgcgcgaaa tcgagtacgc
     4501 ttcggtctgg cgtgatgacg gcgggcgggc gacgcaagcg acgcaatgct ggcatctgct
     4561 gcaagctgcc gacgatgcct ctcggcagga acacgatcgt caccaggatg atgccgccat
     4621 aaatataaag gtgatagtcg tgcaggctgg cgaagaccgt cgggatcagg ttgagaatga
     4681 cgctgccgac cagtggcccg aacagcgtgc cgcttccccc catcagcgcc gatgtcagga
     4741 gcgagaccga gagcgagaag acgaacgtgt cgctgttgat gaagccgttc tgatgggcga
     4801 agaacacgcc gccgatcccg gcgtagaccg agcttatgac gaaggccagg accttatagc
     4861 ggtagacgtt gacgccgacc gactgtgcgg cgatctcgct gccctgcagc gccaggagcg
     4921 tgcgccccat gcgggagcga aacaggttgg cggcgagcag atggcagagg agcgcgacgc
     4981 cggcgatcag ccagaaatac tgcgtcgccg tcatctcgct gccgtcagga agcgtcggcg
     5041 gcgggatcga catgatcccc atcggcccgc cggtgaggct ccagcggttg cttgtcacct
     5101 cggccatgaa gccgaacgcg atcgtcacca tggcgagata ggggcccttg gcgcgcagcg
     5161 agggtaccgc aagcgcggcg ccgaacagcc cggcgagagc gacgccgagc ggggcgctca
     5221 gccagaacgg caagccgagc cgcgccgagg cgagcgcagt ggtgtaggcg ccgaccgcat
     5281 agagtccggc atggccgagc gagatctgcc cggtccagcc gaccaggatg tcgagcccga
     5341 gcgtcacgat gaacaggtag gcgagcgact gcatcaccag aatgaagaag ggatcatcga
     5401 agacgaccgg cagtagtgcc agaatgagcg ccgccagggc gaccagcgcc cagtctttcg
     5461 gacccgagaa ggcgaagatc ggcagctgca tgtcagacct tggtgatctc cctgcgaccc
     5521 atgaggccgg tcggacggat ggcgaggacg atcaggatga aaaagaacgt gatgccttcc
     5581 ttgaggttcg agttgaccat cccaagcaat tgctcgagga gcccgatcac caagccgccg
     5641 atgagaatgc cgatcggatt ctcgagcccc ccgaagatcg ccgccgcgaa cgccttgatg
     5701 ccaggcaccg tccccatata ggcccaggca aaggtgatcg gagcgatgag gatgccgccg
     5761 agccctgcga gcgccgatga gagaacgaac gcgaacacca ccatgcgcgg cacgttgatc
     5821 cccatcaccg ccgccgcatt gcgattgaac gcaaccgcaa tcagcgcctt gccgagcagc
     5881 gagcggcgca ggaacatctg caccgcgaaa accgtgccga gcgcggcgac gatgatgaac
     5941 agttcctgcg ggaagatgcc cgccgagccg atctggagga cggcctcgcc gaatggggag
     6001 ggaaatttga tctgctcagg cccccacaga ttcaccgccg cattcttgag aatgatgctg
     6061 accgcgaccg tgctgagcac ccagctcagg gagagcgcgt ggcgcattac cgggcggatg
     6121 gcgccgcgtt cgagcgcagc gcccatcacc ccagtgagca gtaccgcggc gatcaccgcg
     6181 accgcataag gcaggccgaa ggtcacgtaa caggtcagcc cgaacagcgc cccgatcatc
     6241 aggaactcgc cctggccgaa attgagcgcg ccggtcgtga cgaaggtgat gtaatagccc
     6301 tcggcgatga gggcgtagat actgccaatc gacaggccgc tgaccagcgc ctgccacagt
     6361 tttgcgaaaa acagactcat ctcggcacac ccccggcacg acggatcgcg tggttggcca
     6421 ccctcctgca gacggagggc cggaccaccg aagttgatcc ggggacgggt ttctccgctc
     6481 ccggacagct tcgctgttcg acctccccgt gtcagggggc ggtaaccctc ggcacgttat
     6541 tcggaggcgc gctcaagctt gccgtcgtgc cacaccgcca ggaacccggt gtcgcgaccg
     6601 agcgactcgt ggttggcgtg actgaaggga tgcggcttca tcttggtcac cgcctcggtg
     6661 aaatcgtcga tctcctcgag cgcatcgcgg atcttctgcg ggtcgggacc gaccttgtcg
     6721 agcgcgcgga gcagcaggcg caccccatca taggtttgcg cggtggcgac cggatgaaag
     6781 tcgccgccct gttccttgaa aatgcggtcg accttttcgt gcagcacctt ggcgttggcg
     6841 ctgtggtcgg gcgtgtagga cgtcaccgcc atcaccccct cgacggtcgc cttgccgccg
     6901 agcttgagga agttcggcgc aatcagcgcc caggtcccgg tccacggcgt cttcagcccg
     6961 atcttctgca tgcttttcgc gatctgcacg ccagccggtg ccagtgaata ggcgatgatg
     7021 aattccgcct tggcctcctg catcttggtg agctgcgtcg tcatgtcggt gtcgttctgg
     7081 tcgaacacct cggggccggc cacgaggtcg agattggcct ccttgaccag gcgcagcgcg
     7141 gtatcgcggc cgctctggcc ccagccggtc gaatcgtgca tgaggccgac ccgctgatac
     7201 tgacgaccct tgatgtagtc gatcatgaac gaggtctgga tgccgtcgtt aagcgagacg
     7261 cggaaaatgt agttcttcgg cgcgttcttg tagcgctcga tgatctgctc cgccgtggcg
     7321 acggtgtcca tcagcggtac ctggttcttt tgcgcaatgt cgataatggc gagcgcgacg
     7381 ccggaattga ccggtccgac catggcgaag accttgtcgc gggtgatgag gcgggtgacg
     7441 ttctcgacgc cttttgcggg cttggtctcg tcgtcataga tgacggcttc gaccttcttg
     7501 ctctggtagc cgcccttgtc gttgtattcc ttgagcgcca gttcggcacc catccgcgcg
     7561 cccttgccgt aatcgtcggt gccgccggtc agcgacaagg tgaagccgat cttgaggtcg
     7621 gccgcgactg cggtgctgcc gagaacggcc gccagcagga ggccgatgcc tattgcgccg
     7681 agccgcagcc cggtcgtccg tgcttcgatg ctcattgtcg ttctccctcg gattatcgtt
     7741 attgggcatc ctgtcgctgg tggcggacgc catactgtcg actttaccgc gtcttgagcc
     7801 tcattgaaac agtggcaggg taatcgtcta cgagaaaacg ctgcctcctg tctcccttca
     7861 acgcgcgccg caacttccga cttggtaagc agaccagaag ctaaagcatg agccgccgca
     7921 ggcggaccac cgctggcatg ccccgcaa
//
LOCUS       DATNOI010000431         5654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2352818,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000431 DATNOI010000000
VERSION     DATNOI010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2352818"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VN968_17370"
     CDS             <1..302
                     /locus_tag="VN968_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HXL31039.1"
                     /translation="VVIVGMMGNKDANGFLANFAGLTRHIIAVQIPDRDHAMPLDRLA
                     DAVRQLGMRVETAPGVEAALRSLAGLAYEVPPRILITGSLYLAGHVLSLNGTPPR"
     gene            311..1051
                     /locus_tag="VN968_17375"
     CDS             311..1051
                     /locus_tag="VN968_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXL31040.1"
                     /translation="MRFAAIADVHGNYLALEAVVADIRAQGIDDIVNLGDMASGPLDA
                     RRTMDALMALDAVHVLGNHDRYLIDRLPEKMGSWDRPAHAQLDARHLDWLRAAPPKLV
                     FRDQVLLCHATPDSDEIYWLETVLPDGTVRMSSLEAIEKAAEGIAQRLILCGHSHIAR
                     AVRLADGRMVVNPGSVGSPGYRDVHPFPHVVEAGTPDARYAILELIDGIWRATFRHVP
                     YDHDAMAALARRNGQPELASALATGWIR"
     gene            complement(2598..2918)
                     /gene="trxA"
                     /locus_tag="VN968_17380"
     CDS             complement(2598..2918)
                     /gene="trxA"
                     /locus_tag="VN968_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXL31041.1"
                     /translation="MAVGKVSDADFEAEVLNATGPVVVDFWAEWCGPCRMIAPALDEI
                     SGVMGDKVKIVKLNVDESPKTASKYGVMSIPTLMIFKGGEMASRQVGAAPKQKLQQWI
                     TAAV"
     gene            complement(2953..5654)
                     /gene="addA"
                     /locus_tag="VN968_17385"
                     /pseudo
     CDS             complement(2953..5654)
                     /gene="addA"
                     /locus_tag="VN968_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603936.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
BASE COUNT         1060 a         1772 c         1752 g         1070 t
ORIGIN      
        1 tggtcgtcat cgtcggcatg atgggcaaca aggatgccaa cgggtttctc gccaacttcg
       61 ccgggctgac gcgccacatc attgcggtgc agataccgga tcgcgaccat gcgatgccgc
      121 tggaccggct tgcggatgcc gtgcgtcagc tcggcatgcg ggtggaaacc gcacccggtg
      181 tcgaagcggc gctgcgttcg ctcgcgggcc tcgcctatga agtgccgccg cgcatcctca
      241 tcaccgggtc gctttatctc gccggccatg tgcttagcct gaacggcacg ccgccgcgat
      301 aggtcgattg atgcgctttg ccgcgattgc cgatgtgcac ggaaactatc tcgcgcttga
      361 ggctgttgtt gccgacatcc gcgcgcaggg catcgacgac atcgtcaatc tcggcgacat
      421 ggcgagcgga ccgctcgacg cgcggcggac gatggatgcg ctgatggccc tcgacgccgt
      481 tcatgtgctc ggcaatcatg accgctatct gatcgaccgg ctccccgaga agatgggatc
      541 gtgggatcgg cccgcacatg cgcagctcga tgcacgtcat ctcgactggc tgcgcgcggc
      601 gccgccaaag cttgtgtttc gcgatcaagt cttgctctgc catgcgacgc ccgacagcga
      661 cgagatctat tggctggaga ccgtgttgcc cgacggcacc gttcggatgt cgtcgcttga
      721 agcgatcgag aaggcggccg agggtatcgc gcagcggctg attctctgcg gccacagcca
      781 tatcgcgcgc gcggtccggc ttgccgacgg gcgcatggtc gtcaatcccg gcagcgtcgg
      841 ctctcccggc tatcgcgatg tccatccgtt tccgcatgtg gtggaggccg gcacgccgga
      901 cgcacgctac gccatcctgg agctcatcga tggcatctgg cgcgcaacct tccggcatgt
      961 gccctacgat cacgacgcga tggcggcact ggcgcgccgc aacggccagc ccgaactcgc
     1021 ctccgcgctg gcgacggggt ggatcaggta gtgcgcccaa ccaaacagct gcagattcgg
     1081 ctcacctctc ccgcttgggc tcagcgtcaa tccatggtgg gaccatgtca cgatgacctg
     1141 tcgaagcacg agtggccggg actgattcgc gaagactgat tccaccaagc attacccgca
     1201 cgacatgcga tttcgtgtcc cggacgcgat gcggtgcgca gtaccgcttc gcagagccgg
     1261 gacccacccg caccgcgacg gccgcagcta cacttgcttc aagccagcag ctcacgtcgc
     1321 cgggccgcgg gagtcagggc gtgcaatagt tgacggctac gtaggcagct ttacagcacc
     1381 cggtttcccg gcagtggaaa gcatgttggc ggcgccagaa taaccgctaa ggttgcttag
     1441 cctcggattc accgcaatgt gcaataattt cacattttat tgttgatttt gtgcaactat
     1501 tgcactagcc ttacgtcatt gaagccacat ggcgggcatt gatgctcccc taagtaactg
     1561 taaggtctac atttatccgg gggatcataa tcccccgcac ttccacctaa gaggccccaa
     1621 ttcgagggct tctgtggata tcatgagctt ggaggttatc gcggggaagg cgtcaaagaa
     1681 ggacttggcc gaagtcaaag agtgggccgc aaagccagaa aatctggccg tattggtatc
     1741 agagtggaga aggctcaatg agcgagaatg acatcgttga tagtgggatt ccacttcccc
     1801 gcatggcgac tgtccagccg ctccacgact ttgtgcttcg agttaagtgg gcggaaggtc
     1861 ctagggctgg acgtgaggac ttcgttgacg ttagcccggc catcttcagc tataaggtct
     1921 accgtcctct gagagatgaa aatctttttt tgaccggcac aatcgccgaa gatggagatg
     1981 ctgtcgcatg gggcgatgac gcggatttga taatgtccgc agacaccgtc caatctcttg
     2041 cacaagagag ttggacgcct aaggatttct tggaattctt gacgcgaaac agacttactc
     2101 aagaagccgc cgccgcatta ttgggtagaa gcagaaggca agtcgcttct tacgctacga
     2161 aggggccaat ccctcgcgtc atcgcgctag cgtgctacgg atttgaatcc ctaaggcgcg
     2221 ataggttctt acaggggtcg ggcaacatca acgcgcaacc tcccacaata tacactgccc
     2281 gggtccctga gcagaacgaa caatcccgtt ctcccgctgc ccgcgcaacg ccacgcgaac
     2341 gcgcttgatc atgagcctca aaagccgctg accgctctta tccaaggccc gctcgacaag
     2401 tagccgcaag gtgatctcgc gcgtggtgag cggttcttct cacctgtgca attaattgca
     2461 caagcgtagg cggggcaggc agcaaagccg acaggtggga aggtgagttt tgctgtctaa
     2521 tgcctgtcgc ctcaacgcaa acggccggca atccgccggc cgttgtcgag aataattatc
     2581 gtccgagagc gatcagatca gaccgcagcg gtgatccact gctgcagctt ctgctttggc
     2641 gcggcgccga cctggcgcga ggccatctcg ccgcccttga agatcatcag ggtcgggatc
     2701 gacatcacgc cgtatttcga ggccgtcttg ggactctcgt cgacgttcag cttcacgatc
     2761 ttgaccttgt cgcccatcac gccggaaatc tcgtcgagcg cgggcgcgat catgcggcag
     2821 gggccgcacc attcggccca gaaatcgacc acgaccggac cggtcgcgtt gagcacttcg
     2881 gcctcgaaat cggcgtcaga aaccttgcca acggccattg ggaatacctc gttcagtttg
     2941 ggaagatgcg gcttaaagaa tcgccgccgg gatcatgcag acaacgtatg aacgcgatct
     3001 tgccgggtca agctcggtca tccctttggc tatgggctgc cagctccgcg tccagcgcgg
     3061 gggaagaaat ctccatgaat tcagctgttt cagtccaaag cagtgctgcg cggaccggca
     3121 gctggggata aagcttctgc agcaccccac gatagagtgc gagctggcgg acataggcgg
     3181 caggcgcctc ggcgcccgcg cccggcggag catggttggt cttgtaatcg acaatcagga
     3241 cctcgtccgg ggtcaccacc agccggtcga tttgccccga aaccagtgcc ggcgaccgtc
     3301 ccggccgatc cagccgcccg acaatcgaga cctcggcgcg gctatgaagc gcaaataccg
     3361 gcgcgaaacg cggatccgcg atcagggcga gcacccctcc cgcgagggcc tcccgctcgc
     3421 catcgatcca gccgtcggcg ttgcgggcga ggtatttcag cgccgcgtcg cggcggcgct
     3481 cggccgcgac atcgggcaag gattgcagca gccggtgcac cagcgtcccg cgctgcaagg
     3541 cgcgcgcccg ctccatgacg gactcaccgg tccggatgcg ctggccttcg ttcgcggcag
     3601 gatcggacgg gcgcagcgga cgatcggccc gcaactccgg cggtgccggc gtttgcaacc
     3661 atgagggcag gtcggtcgga gcagtaggca ccgcagctgc ggctttgccg gtgggaactg
     3721 ctgcgtcctc aagccgcgag taacgcttca cctcgccgtc cggcgactcg atagtctgca
     3781 gatgcaatcc ggaattgccg aggcctttgt cgatcagatc gtaccaggag aatcttcgga
     3841 tcgtgttcat gttgcccggc atgcagccgc cgacgatcag gcggtcggcc gcccgcgtca
     3901 tcgccacata cagcagccgg cgatattcgt cctcggtttc aaccagcatg gccgcgcggg
     3961 ccgccgcgac gctggcggga tcgtcggcct tcctgccggc ccagaccacg acatcgggtg
     4021 catgaggcgg tgcgttgtcc agaggcagat gaatgagctt caggcgctgc gtgtccgcgg
     4081 gcgacgtcgt ggtctcgacc agaaaaacca ccgacgcttc cagccccttg gcgccgtgca
     4141 cggtcatgac gcggacttcg tcgcgggaaa tttccatgtc gcgctttact tcggtgtcgg
     4201 ccgcgcgcag ccacgccatg aaaccctgca gcgaggccgg tgctttgcgc tcatagccaa
     4261 gcgccagctc gagaaattcg tcgagcgcgt cgttggcttc atgcccgagc cgcctcagaa
     4321 ttcgcgcgcg gccgccgtcg ccgccgagca gccaggcata aaacgcaaac ggcgtctcct
     4381 gcagcacgcg cacctcgcag acccggaggc gatgcagcgc agcctcgaat ttgccgtcgg
     4441 tcgcggcgtg atgatccaac gcctgccgca gcgagccctt gcgttgccac gccagctgga
     4501 acaggtcgtc gtcggcgagg ccgaacagcg gactcttcag cgccaccgcg agcgccagat
     4561 cgtcctgcgg caacagcagc gcgtcggcca gattcatcag gtcgatgatg gcaatatgtt
     4621 cggtcagctt gagccggtca gcgccggcga ccggaatgtt ggcgtgcttc aatgcctgga
     4681 tcacagcgtc gaaggcgttg ccgcgccgcc gcaccagcac cagcatgtcg ccgtagcgaa
     4741 gcgggcggcg atcgccggtc gggccggtca tggtgccgct gtcgaccagc gacttgatct
     4801 cggcctgaat gcgcttggcg agccggactt ccgggctggt cgccgaaatc ccgtcgaacg
     4861 gcgcccgcca gccctccatg tcctccttct tgtccggcag ctgcaattcc cagaagtcga
     4921 tcaggctcgg cccggcatcg gcgaggaagt tgtggatcgg gtatccgctt tcggcggaat
     4981 ggatgctgcg gtatatcgct tcctcgcgaa acacataatc gaccgattgc aggatcgcag
     5041 gccccgaacg gaacgagtag gtgaacgata ccggatcgaa tttcaggccg gcatcgataa
     5101 actttttttc caggcctctg cggcgagtgt cgaattcgcg cggggccgcg ccctgaaacg
     5161 aaaagatcga ctgcttctcg tcgccgaccg caaatatcgt gcgggtgacg ccgtcacggg
     5221 cgccttcgcc tgacgtgaat tcggaaatga tgtgggcgac gatgtcccat tgccgcgggc
     5281 tggtgtcctg cgcctcgtcg atcaacacat gatcgacgcc gcggtcgagc ttgtaatgca
     5341 cccagccgga cgagacgcgg tccagcatgg ccagcgtctt gtctatcaga tcgtcataat
     5401 cgagcaggcc gcgttccagc ttctcgcgcc ggtaatttgc ggccgccgcg gtcgcaatgt
     5461 gcagcagcgc ctcggtgcgg tcgcgcgcca ccacggcgcg gcggcgttcg atcaggggca
     5521 ggatgcgacg ggcttccgat tcgatcagcc ggccgatggc cggattgttg tcgcagaatt
     5581 tctttgtcac caccgatttg cgcggggtgc gctcatcggt caggaacacg ccgagatatt
     5641 cgtcgacttg cgcg
//
LOCUS       DATNOI010000432         2210 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1218464,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000432 DATNOI010000000
VERSION     DATNOI010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218464"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VN968_17390"
     CDS             <1..660
                     /locus_tag="VN968_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HXL31042.1"
                     /translation="ILVCLAAFVAIWQNGSRGMSRILLALLIDAVVLAYPAYLALQYN
                     KLPPIHDITTDPVDPPRFEALARLRTGDGTNTAVYAGLYSAEQQRLAYPDVETVELDV
                     PVQRAFEVTMQLVNKRKWVVIDERPPQPPRRIGRIEAVARTPIMRFREDVSIRVTPDG
                     EGSRVDIRSSSRYFESDLGSNAARITSLIEDINNAVDNAKPVVKKPQAPVKAPPKVVK
                     K"
     gene            complement(771..1691)
                     /locus_tag="VN968_17395"
     CDS             complement(771..1691)
                     /locus_tag="VN968_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL31043.1"
                     /translation="MSDNDDVPFNRDFPLKPGVVQEVHPGVRRILCNNPSPFTFTGTV
                     SYIVGRGKVAIIDPGPDNEAHAAALLEAVRCETVTHILVTHTHRDHSPNTARIKAATG
                     ATVYAEGPHRASRPRYESEKISSESGADRDFRPDIAVRDGETIEGQGWALQAVATPGH
                     TANHLAFAWQDRKMLFVGDHVMGWSTSIVAPPDGSMIDYMASLERLTGRSEDLYFSGH
                     GPEIPDGPRYVRFLIRHRQAREASILHRLAKGEADIPTMVRAIYIGIDPRLTGAAGYS
                     VLAHLEDLVARGIVATDGDPVIGGTYRLAS"
     gene            complement(1877..>2210)
                     /locus_tag="VN968_17400"
     CDS             complement(1877..>2210)
                     /locus_tag="VN968_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL31044.1"
                     /translation="LAATQLVQKKLADMQTEIALGLQASLRVGRLMDEGKMAPEMISI
                     VKRNNCGKALDIARVARDMHGGNGIQIEYHVMRHAANLETVNTYEGTHDVHALILGRA
                     ITGIQAFS"
BASE COUNT          405 a          711 c          694 g          400 t
ORIGIN      
        1 attctggtgt gcctcgccgc ctttgtcgcg atctggcaga acggatcgcg cggcatgagc
       61 cgcatcctac tggcgctcct gatcgacgcg gtcgtgctgg cctatccggc ctatctcgcc
      121 ctgcaatata acaagctgcc cccgatccac gacatcacca ccgatccggt cgatccgccg
      181 cggttcgagg cgctggcgcg gctgcgcacc ggcgacggca ccaacaccgc agtctacgcc
      241 ggactgtatt ccgcagaaca gcagcggctg gcctatcccg atgtcgagac ggtggaactc
      301 gacgtccccg tgcagcgggc ctttgaggtc acgatgcaac tggtcaacaa gcgcaaatgg
      361 gttgtgatcg acgaacgccc cccgcagccg ccgcgccgca tcggccgcat cgaagcggtg
      421 gcgcggacgc cgatcatgag attccgcgag gatgtctcaa tccgcgtcac gcccgatggc
      481 gagggatcgc gcgtcgatat ccgctcctcg tcccgctatt tcgagagcga tctcggcagc
      541 aacgccgcgc gcatcaccag cctgatcgag gacatcaaca acgccgtcga caacgccaag
      601 ccggtggtga agaagccgca agcaccggtc aaggctccgc ccaaggtggt gaagaaatga
      661 caccctcgtc attccggggc gcgcgatagc ggcgaacccg gaatctcacc cagcaacttc
      721 gagattccgg gtctggccct tcgggccatc ccggaatgac ggtggtcgga ttaagacgcc
      781 agcctgtagg tgcccccgat caccgggtcg ccgtcggtcg caacgatccc gcgtgcgacc
      841 aggtcctcca gatgcgccag cacggaatag cctgcggcac ccgtcagccg cgggtcgatg
      901 ccgatataga tcgcgcgaac catagtggga atgtccgctt cgccttttgc gaggcggtgc
      961 aggatcgagg cttcgcgggc ctgacgatgc cggatcagga aacgcacgta gcgcgggccg
     1021 tccgggattt ccggtccgtg gcccgaaaaa tacagatcct cactccgccc cgtcagccgt
     1081 tccagcgagg ccatgtagtc gatcatcgat ccgtccggcg gcgccacgat cgaggtcgac
     1141 cagcccatca catgatcacc gacgaaaagc atctttcgat cctgccatgc gaaagccaga
     1201 tgattggcgg tgtggcctgg cgtcgccacg gcctgcagcg cccacccctg accctcgatg
     1261 gtctcgccgt cgcgcaccgc gatgtcgggc ctgaagtcgc gatcggcgcc ggattccgaa
     1321 gagatttttt cgctctcata gcgcggccgc gacgcacgat gcgggccctc ggcatagacg
     1381 gtggcgccgg tggccgcctt gatccgcgcg gtgttgggcg agtggtcgcg atgggtgtgc
     1441 gtgaccagaa tatgcgttac ggtttcgcag cgcacggcct ccaatagtgc cgccgcgtgg
     1501 gcctcattgt cggggccggg atcgatgatc gcaaccttgc cacggccgac gatgtaactc
     1561 accgtgccgg tgaaggtgaa cgggctcgga ttgttgcaga gaattctgcg cacgccgggg
     1621 tggacttcct gaaccacgcc gggcttaagc ggaaagtcgc gattgaacgg gacgtcatcg
     1681 ttgtcggaca tgggattcct atgtggctcc gcaatcagcc ccggcgaaat aaccgcattc
     1741 gtcatacccc gcgaaagggg tatccagtag tcgcggacgt cagagataaa gagaaacacc
     1801 tcggagtact ggatcgtccg ctttcgcgga cgatgacgcc ggaagggaag gtcacgcctg
     1861 aggttcctgt caacgcttac gaaaatgcct ggatgccggt gatggcgcgg ccgaggatca
     1921 gcgcgtggac gtcgtgggtg ccttcgtagg tgttgacggt ttccaggttc gcggcgtggc
     1981 gcatcacatg atattcgatc tggatgccgt tgccgccgtg catgtcgcgg gccacccgcg
     2041 cgatatcgag cgccttgccg caattgttgc gcttgacgat ggaaatcatc tccggcgcca
     2101 tcttgccctc gtccatcagc cggccgaccc gcagcgaggc ctgcaggccg agcgcgattt
     2161 cggtctgcat gtccgcgagc tttttctgca ccaattgcgt cgccgccaag
//
LOCUS       DATNOI010000433         8226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_450008,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000433 DATNOI010000000
VERSION     DATNOI010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8226
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450008"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VN968_17405"
     CDS             complement(<1..399)
                     /locus_tag="VN968_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HXL31045.1"
                     /translation="MPSGANVVLYAGLLIGLVYGAVGLLSGFCLMSSLRGWWAQGDSR
                     LIRSYALALGVAIAATQLLAARGVVDLGKSIYLQPSFSAPLMFFGGLLFGYGMVLSNG
                     CGSRALVLLRRGNLRSFVVVIVLGITAQMTL"
     gene            complement(415..1443)
                     /locus_tag="VN968_17410"
     CDS             complement(415..1443)
                     /locus_tag="VN968_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HXL31046.1"
                     /translation="MRPASKKHHSQPVMVEARCLRLLAKADDPASLAPAIERRVLSRA
                     ANEVLIEIAAAAVNPSDVKAATGLMAYAVFPRTPGRDYAGVVIDGPSGWIGREVFGSS
                     GDLGIRRDGTHATHLAVEADAVVEKPKNLSWDEAAGIGVPFVTAMEGFRRAGMPSKDE
                     TVLVMGVNGKVGQAAVQIASWHGARVIGVVRRSESYEGHANAGVDVIDASATDVATRV
                     RELTDGKGADIVFNTVGDPYFQAAHQSLALRGRQILIAAIDRIVQFNILEFYRGQHTY
                     VGIDTLGLSSVATGAVLKELVPGFASGHLKPFPILPAAIYSLENARSAFVAVAGSSRD
                     RIILRPAQ"
     gene            1634..2074
                     /gene="paaI"
                     /locus_tag="VN968_17415"
     CDS             1634..2074
                     /gene="paaI"
                     /locus_tag="VN968_17415"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HXL31047.1"
                     /translation="MNLKATLSPDELARACVEAMWKEDDASKGLGMEILQIRAGEAVL
                     TMTVKPNMVNGHGIAHGGFIFALADSAFAFACNSYNERAVTAQCNISFIRPGKLGDRL
                     TATAREISRTGRSGIYDVRVTVDDVAIAELRGHSRTVGGAWVPA"
     gene            2133..3464
                     /gene="paaK"
                     /locus_tag="VN968_17420"
     CDS             2133..3464
                     /gene="paaK"
                     /locus_tag="VN968_17420"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HXL31048.1"
                     /translation="MASTNQQIRPGSYRAEMDDAERASRDEIIALQTRRLGWTLEHAY
                     DNVAHYRKAFDKAGVKPQDFRQLSDLGNFPFTAKTDLRDNYPFNMFAVPREKLVRVHA
                     SSGTTGKPIVVGYTRADIDMWSDVMARSIRAAGGRTGMIIHNAYGYGLFTGGLGVHYG
                     AERLGCTVVPVSGGMTERQVQLINDFKPDIITVTPSYMLAILDEFRKQGLEPRKSSLK
                     FGIFGAEPWTNAMRAEIEAAFAMDATDIYGLSEVIGPGVAQECVETKDGLHVWEDHFY
                     PEVIDPETGAVLPDGEKGELVFTSLTKEAFPIIRYRTRDLTRLLPGTARPGMRRMEKV
                     TGRSDDMIILRGVNVFPTQIEEVLLATDWCGGHFIIELTREGRMDEMTVLAEARPESW
                     DGNGLVEHAERVTTYIKNTIGITARIKAVAPETLERSLGKAKRVFDKRPKE"
     gene            3594..5024
                     /gene="iaaH"
                     /locus_tag="VN968_17425"
     CDS             3594..5024
                     /gene="iaaH"
                     /locus_tag="VN968_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indoleacetamide hydrolase"
                     /protein_id="HXL31049.1"
                     /translation="MADTTLPSVAEIVADIRSKKMTAVSAVRAALERAEQAKDLNAFI
                     VVNRDGALTAAGRVDSGETTGALAGLPIVVKDNINTADMPTSGGTPALQNARPTRNAP
                     SLQKLLDAGAIIIGKTNMHELAFGITSTNLSSFAGPVKNPYDKTRIPGGSSGGTSAAI
                     AARIVTCGLGSDTGGSTRVPAALTGTVGLRPSVGNGGAQRRYHDDNLVVPISHTRDTA
                     GPMGRTVADVALLDSVITGTPMAKAEPLRGKRLGIPASFWSGLDRDVDAVAKAAREKL
                     ADAGVVLVDADMAGLFEQNGKVSFVVALHEPIADIPAYLHASGIEGITLADIAGKIAS
                     PDVQGAFGAITADAFGAAYQDAITVQRPALQKIYEAYFRDHNLDAILFPTTIAAAPVI
                     DIEKGSGQMSIDGGKPVPTFDTMIRNTDPGSNAGIPGLSLFAGMTPGGLPVGLEIDGP
                     VGSDAKLLGLGLSIEGILGSAPPPKG"
     gene            complement(5083..5853)
                     /locus_tag="VN968_17430"
     CDS             complement(5083..5853)
                     /locus_tag="VN968_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL31050.1"
                     /translation="MSATAQRLRPPSKTLMFLEGRAIHELGAFLGALPLLSLAPKGDG
                     HPVLVLPGLVASDTSTRPLRSFLKNRGYAVSGWRQGRNLGLRDGVQRAMVDLVHELTD
                     AGGRKISLVGWSLGGLYARQLAKMMPDRVRSVITLGSPFAGHPKATNAWRVYEMASGR
                     RADEEDGRFGGSLAGTPPVPTTAIFSRTDGICAWQGCMEKNSATSESIEVESSHCGMG
                     YHPAAVYAVADRLAQPEGKWAPFDRSGWRSLVYPDPAR"
     gene            complement(6053..7339)
                     /locus_tag="VN968_17435"
     CDS             complement(6053..7339)
                     /locus_tag="VN968_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HXL31051.1"
                     /translation="MTVPGTFSFDAPGDAARAAELRRVKALATLVLAGTLAVFITAKS
                     LLHVHPVFGFVAAFAEAATIGGLADWYAVVALFRRPLGLPIPHTAIIQANQHRIADKL
                     GEFIEVHFLEAAPVDAKLRQIDFGSFIADWLRDRKRSTDLARFTLRLLPEAVSATETS
                     GLMTFITRRISTQLQSIDLAPLAAGTLRGFVAEGRHQGLLDDILRAVQQTLTQPETMA
                     VIRDKIRGEMPTLLKLYRADKFLVNRIVASATTFFEEVRNDPKHPFRGEFDRMILSFV
                     DRLGSDQNFADRLDGLKRDLLARPELGALARNIWSNARSFIERSASGETQVLQQHLTR
                     MFMEAGEALAGDSELRGEINQGLVAVFRSFIADQKSGVSSFISDQVKAWDMGQLISLI
                     EINIGKDLQYIRFNGSLIGGLAGLSLYTIEVLLRWL"
     gene            complement(7634..8116)
                     /locus_tag="VN968_17440"
     CDS             complement(7634..8116)
                     /locus_tag="VN968_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HXL31052.1"
                     /translation="MTTETNSVLNTVKEAFAPVTDALKNLQNLEVPEAAREFVKRTAS
                     TAKDRAADLHAGSEKVTAAIETAVADSVSEAAKISRNIQQAIYQDTEAFFAGIDKLAS
                     AKSLNEAVQIQSDMVRARGEVFMSRAKSTTEYLGKLVADGAKTAQDNLSKVASYSKTA
                     "
BASE COUNT         1574 a         2612 c         2596 g         1444 t
ORIGIN      
        1 cagcgtcatt tgcgctgtta tgccgagcac gatcaccacc acgaacgagc gtagattgcc
       61 gcgcctgagc agtaccagcg cacgcgagcc gcagccgttc gacagcacca tgccgtaacc
      121 gaacagcaga ccgccaaaga acatcagcgg cgctgaaaac gacggctgca ggtaaatcga
      181 tttgccgaga tcgacaacac cgcgtgccgc caagagctgc gtcgccgcga tcgccacgcc
      241 aagcgccagc gcataacttc ggatcaggcg gctgtcgccc tgcgcccacc agccgcgcag
      301 gctgctcatc aggcagaacc cgctcaagag cccgaccgcg ccatagacga ggccgatcag
      361 caggccggca tagagcacca cgttggcacc tgatggcact ggtcttgtcc cttcttattg
      421 cgccgggcga aggatgatgc gatcgcgcga cgaaccggcg accgcgacaa aggcgcttct
      481 ggcgttttcc agtgaataga tcgcggctgg cagaatcgga aacggcttga gatgtccgct
      541 ggcaaaaccg ggaaccagtt ctttcaaaac cgcgcccgtc gccaccgacg atagccccag
      601 cgtgtcgata ccgacataag tgtgctggcc acgatagaat tccaggatat tgaactgcac
      661 gatccggtcg atcgcggcga tcaggatttg ccgcccccgc aatgccagcg attggtgcgc
      721 cgcctgaaaa tagggatcgc cgacggtatt gaacacgatg tcggcgccct tgccgtccgt
      781 cagctcgcga acgcgcgtcg caacgtccgt cgcggaggcg tcgatcacgt caaccccggc
      841 attggcgtgc ccctcgtagg actcgcttct gcgcaccaca ccgatcacgc gcgcgccgtg
      901 ccagctcgcg atctgcaccg ctgcctgccc gaccttgccg ttgacgccca tcaccagcac
      961 cgtctcgtcc ttgcttggca tgccggcgcg gcgaaagcct tccatcgccg tgacaaaggg
     1021 aacgccgatc ccggcggcct catcccacga caggtttttc ggtttttcca ccacagcatc
     1081 ggcttcaacc gcgagatgcg tggcatgggt gccgtcgcgc cggatgccga gatcgcccga
     1141 cgagccgaac acctcgcgcc cgatccaacc ggatgggcca tcgatcacga cgcccgcgta
     1201 atcgcggccg ggcgtgcgcg gaaatacggc gtaagccatc aggccggtcg cggccttgac
     1261 gtcggacgga ttcacggccg ccgccgcgat ttcgatcagc acctcgttcg cggcccgcga
     1321 cagcacgcgc cgctcgatcg caggcgccag cgatgccgga tcgtcagcct tcgccagcag
     1381 gcgcaggcag cgcgcctcga ccataacagg ctgactatgg tgttttttgg aagctggccg
     1441 catgggtgtt ccgatttctg gagcgacttt cgaacgcgat ttgacgcctg ccgggatcgt
     1501 tgacaccatg gtggcttcgc atcatgacta accgactgat cgcttagttc aagaggcttg
     1561 ttgcaacggg ctggagaacg tcttacccga acctgatcga cgggcggccg tgcaaattgg
     1621 cgaggacacc gcagtgaacc tcaaggccac gctttccccc gacgagctgg cgcgcgcttg
     1681 tgtggaagcg atgtggaagg aagacgacgc cagcaagggt ctcggcatgg agatcctgca
     1741 gatcagggca ggcgaagccg tcctgacgat gacggtcaag ccgaacatgg tcaacggcca
     1801 cggcatcgcc catggcggct tcattttcgc cctggccgac tcggcattcg cctttgcctg
     1861 caattcctac aatgagcgcg cggtcacggc gcagtgcaac atctccttta tcaggccggg
     1921 caagctcggc gatcggttga cagcgacggc acgggaaatc tcgcgaaccg gacgttccgg
     1981 gatttacgat gtgcgcgtca ccgtcgatga tgtcgcgatt gccgagttgc gcggccattc
     2041 ccgcaccgtc ggcggcgcat gggtgccggc ctgacgccga tgcctggtcg atatgacgct
     2101 gaaatgaacc tctgattgta gggaaccgcg cgatggcttc gaccaaccag caaatcagac
     2161 ccggcagcta tcgcgccgag atggatgatg ccgagcgtgc atcgcgcgat gaaatcatcg
     2221 cattgcagac caggcgcctc ggctggacgc tggagcacgc ctacgacaac gtcgcccatt
     2281 accgaaaggc gttcgacaag gccggtgtca agcctcagga ttttcggcaa ctgtcggatc
     2341 tcggtaactt tccattcacg gccaaaaccg acctgcgcga caattacccg ttcaacatgt
     2401 tcgcggtacc gcgcgagaag ctggttcgcg tccacgcctc ctccggcacc accggcaagc
     2461 cgatcgtggt cggatacaca agagccgata tcgacatgtg gtcggacgtc atggcgcgct
     2521 cgatccgcgc cgcgggcggc cgcaccggca tgatcatcca caacgcctat ggctatggcc
     2581 tgttcaccgg cggccttggc gtgcattacg gcgcggagcg gctcggctgc accgtggttc
     2641 cggtctccgg cggcatgacc gagcggcagg tgcagctgat caacgacttc aagcccgaca
     2701 tcatcacggt gacgccaagc tatatgctgg caatcctgga cgaattcaga aagcagggcc
     2761 tggaacctcg caagtcgtcg ttgaaattcg gcatcttcgg cgccgagccc tggaccaacg
     2821 cgatgcgcgc cgagatcgag gcggcgttcg ctatggacgc caccgatatc tacggcttgt
     2881 ccgaagtgat cggccccggc gtcgcgcagg agtgcgtcga gaccaaggac ggcctgcatg
     2941 tctgggagga ccacttctat ccggaggtga tcgatcccga gaccggcgcg gtactgcccg
     3001 acggtgaaaa gggcgagctt gttttcacct cgctgaccaa ggaagcgttc ccgatcatcc
     3061 gctaccgcac ccgcgacctg acgcggctgc tgccgggcac ggcgcggccc ggcatgcggc
     3121 gcatggagaa ggtcacggga cgttccgatg acatgatcat cctgcgcggc gtcaatgtgt
     3181 tcccgaccca gatcgaggaa gtgctgctcg ccaccgactg gtgcggcggt catttcatca
     3241 tcgaattgac gcgcgaaggg cggatggacg agatgacggt gctggcggaa gcgcgaccgg
     3301 aaagctggga cggcaacggg cttgtcgaac acgccgagcg ggtcaccacc tatatcaaga
     3361 acaccattgg catcaccgcg cgcatcaagg ccgtggcgcc cgagacgctt gagcgctcgc
     3421 tcggcaaggc caagcgcgta ttcgacaagc ggccgaagga atgaacgtta gccgcagcag
     3481 ctcgagagga atggctgagc gacagtccct aacgccgcga tatgctggca tcgtcgatag
     3541 tcctcgccgc tggtgctgcg gcgctccctt ccctcgcatc tgtgagttca gccatggccg
     3601 ataccactct tcccagcgtc gccgagatcg tcgctgacat ccgcagcaag aagatgacgg
     3661 ccgtcagcgc cgtgcgggcc gcgctcgaac gcgcggagca agccaaggac ctcaacgcgt
     3721 tcattgtcgt gaacagggat ggcgcgctga cggcggcagg ccgggtcgat tcgggcgaga
     3781 cgaccggtgc cctcgccggt ctgcctatcg tcgtcaagga caacatcaac accgctgaca
     3841 tgccgacgtc aggcggcacg ccggcgctgc agaatgcgcg gccgaccagg aatgcgccgt
     3901 cgctgcagaa attgctggat gccggcgcta tcatcatcgg caaaaccaat atgcatgaac
     3961 tcgcattcgg gatcaccagc accaatcttt cgtcattcgc agggccggtg aaaaatccct
     4021 atgacaagac ccggattccc ggcggctcat cgggcggaac gtcggcggcc atcgcggcgc
     4081 gcatcgtgac ttgtggattg ggatccgata ccggcggttc gacccgggtg ccggcggcgc
     4141 tgaccggtac cgtgggctta aggccctcgg tcggcaatgg cggcgcgcaa cggcgttacc
     4201 acgatgacaa tttagtggtc ccgatcagtc acacccgcga caccgccggt ccgatgggcc
     4261 gcacggtcgc ggacgtggca ttgctggatt ccgtcatcac cggcaccccg atggccaagg
     4321 cggagccact gcggggcaag cggctcggca ttcccgcatc gttctggagc gggctcgacc
     4381 gcgacgttga cgccgtcgcc aaggccgcgc gcgaaaaact cgcggacgct ggcgtcgttc
     4441 tggtcgatgc cgacatggct ggactgttcg aacagaacgg caaggtatcg ttcgtcgtcg
     4501 cgttgcatga accgattgcg gatatccccg cctatctcca tgcgtccggc atcgagggca
     4561 tcacgctggc cgacatcgcc ggcaagatcg ccagccccga cgtccagggc gcattcggcg
     4621 ccatcaccgc ggacgccttt ggcgcggcct atcaggatgc gatcacggta cagcggccgg
     4681 cgttgcagaa gatctacgag gcgtatttcc gcgaccacaa cctggacgcc atcctgtttc
     4741 caaccacgat tgccgcagcg cctgtgatcg acatcgagaa gggatcgggc cagatgtcga
     4801 tcgatggcgg caagccggtg ccgacgttcg acaccatgat ccgcaacacc gatcccggca
     4861 gcaacgccgg tattcccggc ctgtcgttgt ttgcaggcat gacgcccggc ggcttgccgg
     4921 tcggactgga gatcgatggt cccgtcggca gcgatgcgaa gctgctcggg ctcgggcttt
     4981 cgatcgaagg catcctgggc tcggcgccgc cgccgaaggg gtaaatctca gccggtcatt
     5041 ccccggttgg attcacagca acgccggccg gcctcggtcg gatcacctgg cgggatcggg
     5101 atagaccagg ctgcgccagc cgctgcggtc gaacggcgcc catttgcctt cgggctgcgc
     5161 cagacgatcg gccaccgcgt aaacggcagc gggatgatag cccatgccgc aatggctgct
     5221 ttcgacctcg atgctttcgg acgtcgcgga gttcttttcc atgcagccct gccacgcgca
     5281 gatgccgtcg gtgcggctga agatcgcggt ggtcggcacc ggcggcgtgc ccgccagcga
     5341 tccaccgaac cgtccgtcct cttcgtcggc gcggcggccg ctcgccatct catagacccg
     5401 ccaggcgttg gtcgccttgg ggtgacccgc gaacggactg ccgagcgtga tcacggagcg
     5461 cacgcgatcc ggcatcatct tggcgagctg gcgcgcgtag agaccgccaa gactccagcc
     5521 gaccaggctg atcttgcgcc caccggcgtc ggtgagctca tgcaccagat cgaccatcgc
     5581 acgctgcaca ccgtcgcgca ggccaagatt gcggccctgc cgccagccgc tgaccgcata
     5641 gccgcggttc ttcaaaaagc tgcgcagcgg gcgcgtcgag gtatccgagg cgaccagccc
     5701 gggcaacacc agtaccggat gaccgtcgcc tttcggcgcc aggctgagca gcggcaatgc
     5761 gccgagaaac gcaccaagct catggatggc ccgcccctcc agaaacatca gggttttcga
     5821 cggcgggcgc agcctctggg cagtggcgga cattgtagct cctttcagag ccggcccctt
     5881 aagtctcggc agccggctaa tggaccatca ggaagacgca aacctgttag aatcgttccg
     5941 caatgcagca atctgcagtt taaagctaag gagccgcttc tggcgtttca agctgctcat
     6001 cttgacgcgg ccagggatcg ccgcacttgg gagccttgca gtgatctaaa agtcacagcc
     6061 aacgcagcag gacttcgatg gtgtagaggc tcaatcccgc cagacctccg atcagcgagc
     6121 cgttgaagcg gatgtattgc agatccttgc cgatgttgat ttcgatcagc gaaatcagct
     6181 gccccatgtc ccaggccttg acctgatcgg agatgaagct cgagacgccg cttttctgat
     6241 cggcgatgaa gcttctgaac acggcaacca gaccctggtt gatctcgccc cttagctcgg
     6301 aatcgccagc gagcgcctcg cctgcttcca tgaacattcg ggtgaggtgt tgctgcagca
     6361 cctgggtctc gccgctggcg ctgcgttcga taaacgatcg cgcgttcgac cagatattgc
     6421 gcgcgagggc gccgagttcg ggacgcgcca gcaggtcgcg cttcaacccg tcgagacgat
     6481 cggcaaagtt ttgatcgctg ccgagccggt ccacgaacga caatatcatg cgatcaaatt
     6541 cgccccggaa tggatgcttg ggatcgttgc gaacttcttc gaaaaacgtg gtcgctgatg
     6601 cgacgatcct gttcaccaga aacttgtcgg cgcggtagag tttgagcagc gtcggcattt
     6661 caccgcggat tttgtcgcgg atgacggcca tggtttccgg ctgggtcagc gtctgctgga
     6721 cggcgcgcag gatgtcatcg agcaacccct gatgccggcc ctcggcgacg aaaccgcgca
     6781 atgtaccggc agcgagcggc gccagatcga tcgactgcaa ctgggtactg atgcggcggg
     6841 tgatgaacgt catcaggccc gaggtttcgg tcgccgagac ggcttcgggc agaagccgca
     6901 gcgtgaagcg ggcgagatcg gtgctgcgct tgcggtcgcg cagccagtcg gcgatgaagg
     6961 agccgaaatc gatctggcgc agcttggcgt cgacgggggc ggcttcgaga aaatgcacct
     7021 cgatgaattc gccgagcttg tccgcgatgc ggtgctgatt ggcctggatg atcgcggtgt
     7081 gcgggatcgg caatcccaac ggcctgcgga acagcgccac cacggcgtac cagtcggcga
     7141 ggccaccgat ggtcgcggct tccgcgaacg ccgcgacaaa gccgaacacc ggatgcacat
     7201 gcagcaagct cttggccgtg atgaacacgg cgagagtgcc cgctagcacc agcgtggcca
     7261 gcgccttgac gcgccgcaac tcggccgcgc gcgcagcatc gccgggagcg tcgaacgaga
     7321 atgtgccggg gacggtcatg gcggctttcg agctaaggga accggtacat aggagctgtg
     7381 cgtatcgcca tcatcttgac cgaagcaatg catcgtgtcc cggacgcggt gcaacgcatc
     7441 ttgcggtgcg ccgcagagcc gggacccaca aatcggcggc ggtgaccccg gatcagcagc
     7501 gcaccacgcc gcaagagcgg cgcgctgcgc agcatccggg gaacgccgcc tgcagggggc
     7561 atgcccgtca aaaagaaggc ccgcatcaag cgggccttcg aaagatcggc gggtcgactg
     7621 atccgaaaag cgatcaggcg gtcttgctgt aagacgcgac cttcgagagg ttgtcctggg
     7681 cggtcttcgc accgtcggcg acgagcttgc cgagatattc ggtggtggac ttggcccgcg
     7741 acatgaacac ttcgccgcgc gcacggacca tatcggactg gatctggacc gcttcattga
     7801 gcgacttggc ggaggccagt ttgtcgatgc ctgcgaagaa ggcttcggtg tcctgataga
     7861 tcgcctgctg gatgttgcgg ctgatcttgg cggcctcgct gaccgaatcg gccaccgcgg
     7921 tctcgatcgc ggcagtcacc ttctccgaac ctgcatgcag gtcggcggcg cggtccttgg
     7981 cggtagaggc agtgcgcttg acgaattcac gggcagcctc gggaacttcc aggttctgga
     8041 ggttcttgag cgcgtcggtg acgggcgcga aagcttcctt gacggtgttc agtactgaat
     8101 tggtttcggt ggtcattgcg gtctctccat cctcaggttt tgagctatgg gctcaccccg
     8161 tccggattgg cccggggcga gatccttgtg ccatagttaa tggtgcatcg caacatcaat
     8221 gttgcg
//
LOCUS       DATNOI010000434         2507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_380567,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000434 DATNOI010000000
VERSION     DATNOI010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380567"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VN968_17445"
     CDS             <1..282
                     /locus_tag="VN968_17445"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HXL31053.1"
                     /translation="RVKEDVDKLKAENPSASVPVDLVTTSGSGLDPDISPEGALFQVP
                     RIAGARNMPEDRIRQLVNENTKGRFAGLLGEPRVNVLALNLALDAAAPK"
     gene            325..>2507
                     /locus_tag="VN968_17450"
     CDS             325..>2507
                     /locus_tag="VN968_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HXL31054.1"
                     /translation="MADQRRDLEKRPSPDALLDAARRENDQIGRLKIFVGAAPGVGKT
                     YEMLQNAHAKRKAGVDVVVGVVETHGRPETETLLQGLEVLPRNRIEYKDQILEEMDLD
                     ALIARHPRIALVDELAHTNAPGSRHPKRYLDVEELLSHGIDVYTAVNIQHIESLNDVV
                     AQITHVRVRETVPDSVFDRADAIELIDLTPDDLIQRLKEGKVYVPKQAERALEHYFSP
                     GNLTALRELALRRTAERVDEQLLTHMQANAIAGPWAAGERILVCLSEDPRAAGLVRYT
                     KRLADRLHAPWTALSIETRRSLQLTEEQRDRLADTLRLAEALGGEALTIPAVGRRIAD
                     DVIGFAHANNVTQIIIGKSTRSWWFELTRGSVVHDLVRRAGNISVNVIAGEELASEPV
                     PKKTVRTAERPEPFNPRPYLMALAIVAVGLGIAELIQPRFGIENVDLVFFTAVVVVAV
                     RYGLWPSLLACVVASLCYNFFFLPPIYTFTITDPTNVAAFFFFMLIALLVSNVAARVR
                     TQADSAIGRVRTTESLYAFSRKLAGTATLDDVLWATAYQTALMLKVRVVLLLPEDGVL
                     TVKAGYPPEDELDKADLAAANWAWGNDRPAGRGSDTLPGAKRLFLPMRTGRGPIGVIG
                     IDDDRTGPLLTPDQRRLLDALVDQGALAIERVLLVEDMDRVKRTVESERLRSALLTSI
                     SHDLKTPLASVLGAASTMRDLSGGLSELEKRDLLATVIDESERLNR"
BASE COUNT          442 a          807 c          820 g          438 t
ORIGIN      
        1 cgggtcaagg aagatgtcga caagttgaag gccgagaatc cttccgcttc cgtcccggtc
       61 gatctcgtca cgacttcggg cagcggcctc gatcccgata tctcgccgga gggcgcgtta
      121 ttccaggtgc cgcgtattgc aggagcgcgg aatatgccgg aagaccgcat ccgccagttg
      181 gttaacgaga acacaaaggg ccgcttcgcc gggttgctag gcgagccgcg ggtcaacgtg
      241 ctggcgctga acctggcgct ggatgctgcc gcaccgaaat agagccgcct aggcggtatc
      301 aggcgcgagg actatattgc ccgcatggca gatcaacgcc gcgaccttga gaaacgaccc
      361 tctccggatg cgctgctgga tgccgcccgg cgagaaaacg atcagatcgg ccggctgaaa
      421 atcttcgtcg gcgccgcccc cggcgtcggc aagacctatg aaatgttgca gaatgcgcat
      481 gccaaacgaa aggccggagt cgacgtggtc gttggcgtgg tcgaaaccca cggacggccg
      541 gagaccgaga cgctgctgca gggtctggaa gttctcccgc gcaaccggat cgaatacaag
      601 gaccagattc tcgaggagat ggatctcgat gcgctgatcg cgcgccatcc gcggatcgcg
      661 ctggtcgatg aactagccca caccaacgca cccggcagcc gtcatcccaa gcgctatctg
      721 gacgtcgagg aactgctgtc ccacggcatc gacgtctaca ccgcggtcaa tatccagcac
      781 atcgagagcc tcaacgacgt cgtcgcccag atcacgcatg tgcgggtgcg cgagaccgtc
      841 cccgattcgg tgttcgaccg cgccgacgcg atcgagctga tcgatctgac gccggacgac
      901 ctgattcagc ggctgaaaga gggcaaggtc tacgttccca agcaggctga gcgcgcgctc
      961 gagcattatt tttcgccggg gaatctgacc gcattgcgcg aactggcgct gcggcggacc
     1021 gccgagcgcg tcgacgaaca attgctcacg cacatgcagg ccaacgccat cgcgggcccg
     1081 tgggcggcgg gcgagcgcat cctggtttgc cttagcgagg acccgcgggc ggccgggctc
     1141 gtgcgctaca ccaagcggct ggcggaccgc ctgcacgcgc cctggaccgc gctgagcatc
     1201 gagacgcggc ggagcctgca attgacggaa gagcaacggg atcggcttgc cgacaccctg
     1261 cgtttggccg aggcgctggg aggcgaggcg ctcaccattc ccgctgtcgg acgccgaatc
     1321 gccgacgatg tcattggttt cgcgcacgcc aataacgtga cccagatcat catcggcaag
     1381 tcgacgcgtt cctggtggtt cgagctgaca cgcggctcgg tggtgcatga tctggtgcga
     1441 cgcgccggca atatcagcgt caacgtgatc gccggcgagg aattggcgtc ggagcctgtt
     1501 ccaaagaaga cggtgcgcac ggccgaacga cccgagccat tcaatccgcg cccgtatttg
     1561 atggcgctcg cgatcgtagc cgttggcctc ggtattgccg aactgatcca gccccggttc
     1621 ggcatcgaga acgtcgacct ggtgttcttc accgcggtgg tggtggtcgc ggtccgctac
     1681 ggcctgtggc cgtcattgct ggcctgcgtc gtagcctcgc tctgctacaa cttctttttc
     1741 ctgccgccga tctatacctt caccatcacc gacccgacca acgtggcggc gttcttcttc
     1801 ttcatgctga tcgcccttct ggtttccaat gttgcggcgc gcgtgcgcac ccaggccgat
     1861 tccgcgatcg gcagggtgcg gaccacggag tccctttatg cctttagccg caagcttgcg
     1921 ggcaccgcca cgctcgacga tgtgttgtgg gcgaccgcct accagacggc gttgatgctg
     1981 aaggtccggg tggtgctgtt gctgcccgag gacggcgtcc tgaccgtcaa ggcgggctac
     2041 ccgccggagg atgagctgga caaggccgac ctcgccgcgg cgaactgggc gtggggcaac
     2101 gaccgcccgg ccggtcgcgg ctcggacacg ctgcccggcg ccaagcgcct gttccttccg
     2161 atgcgaaccg gacgcggccc gatcggcgtg atcgggatcg acgatgacag gaccgggccg
     2221 ctgctgacgc cggaccagcg ccgattgctg gatgccctgg tggaccaggg cgcgcttgcg
     2281 atcgagcgcg tgctgctggt cgaggacatg gatcgcgtca agcgcaccgt ggagtcggag
     2341 cgtttgcgct cggcgctctt gacctcgatc tcgcacgatc tgaagacgcc gctggcctcg
     2401 gtgctcggcg ccgcctccac gatgcgcgat ctctccggcg gcctgagcga attggaaaag
     2461 cgggacctgc tcgccaccgt gatcgacgag tccgagcggc tcaaccg
//
LOCUS       DATNOI010000435         2263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_297700,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000435 DATNOI010000000
VERSION     DATNOI010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297700"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VN968_17455"
     CDS             complement(<1..327)
                     /locus_tag="VN968_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL31055.1"
                     /translation="MDFALTANQESIRDAVARICARFDDAYWLKKDKEGGFPADFHKA
                     LADAGWLGICIPEEYGGSGLGITDAAIMMRTISESGAGMSGASAVHMNVFGLNPVVVF
                     GTDEQRR"
     gene            complement(332..1540)
                     /locus_tag="VN968_17460"
     CDS             complement(332..1540)
                     /locus_tag="VN968_17460"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXL31056.1"
                     /translation="MGPLQGIKVVDMTTVLMGPYATQMLGDYGADVIKIETTDGDVTR
                     QIGPTRHPGMGPVFLNTNRSKRSICLDLKKAAGRDAVLRLIASADVLVYNVRPQAMAR
                     LQLGYDVVTKINPRLIYAGVFGFGQDGPYAAKPAYDDLIQGATALPALMAQTNDGVPR
                     YVPNAMVDRIVGLAAVGAICASLVHRERTGRGQRVDIPMFETMAGFVMGDHMGGLTYE
                     PPLDRGGYARHLARDRRPYKTLDGYICVIVYNDKQWENFFKATGRDDLRSDPKFATFA
                     GRAVNIDTVYGELARILETRTTAEWIELLENADVPVMPMHDLESILQDPHLVATDFFP
                     VVDHPTEGRIRNMKVAAAWSETFADPTRLAPRLNQHGAEILREAGFSSDEIATLARDG
                     VTRAPHSPQD"
     gene            complement(2031..>2263)
                     /locus_tag="VN968_17465"
     CDS             complement(2031..>2263)
                     /locus_tag="VN968_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL31057.1"
                     /translation="LLLTDILLPGGMTGFDLARQARRRWPDLPILFMSGFADPSLVPD
                     DFRTNTKLLAKPFRLGQLSEAIVFALAGTKVT"
BASE COUNT          418 a          691 c          733 g          421 t
ORIGIN      
        1 tctgcgctgt tcgtcggtgc cgaacaccac caccgggttg agcccgaaca cattcatatg
       61 gacggctgac gccccggaca tgccggcgcc ggactccgaa atggtccgca tcatgatcgc
      121 ggcatcggtg atgccgaggc ccgagccgcc atattcttcg gggatgcaga tgccgagcca
      181 gccggcatcc gccagcgcct tgtggaagtc ggccgggaaa ccgccctcct tgtccttctt
      241 cagccaatag gcatcgtcga agcgcgcgca gatcctggcc acggcgtcgc ggatcgattc
      301 ctggttggcg gtgagtgcaa aatccatcgg atcagtcctg cgggctgtgc ggcgctcggg
      361 tgacgccgtc gcgcgcgagc gttgcaatct cgtcggagga gaagccggct tcgcgcaaaa
      421 tctcggcacc gtgctggttg agccgtggcg ccagccgcgt cgggtcggca aatgtctccg
      481 accaggcggc ggccaccttc atgtttcgaa tccgcccctc ggtcggatgg tcgacgacgg
      541 ggaagaaatc cgtcgcgacc agatgcggat cctgcaggat gctctccaga tcatgcatcg
      601 gcatcacagg cacgtcggcg ttttcgagca gctcaatcca ttcggcggtg gttcgtgttt
      661 cgaggatgcg ggccaattcg ccatagacgg tatcaatatt gacggcgcgc cccgcgaagg
      721 tcgcgaattt gggatcgctg cgcagatcgt cgcggccggt ggccttgaag aagttttccc
      781 attgcttgtc attgtagacg atcacgcaga tatagccgtc caacgtcttg tacggcctgc
      841 ggtcacgcgc cagatgccgc gcgtagccgc ctctgtccag cggcggctcg taagtcaggc
      901 cgcccatgtg atcgcccatg acaaaccccg ccatggtctc gaacatcggg atatcgacgc
      961 gctgaccgcg cccggtgcgc tcgcgatgca ccaggctggc gcagatcgcg ccgacggccg
     1021 cgagcccgac gatccgatcg accatcgcgt tggggacata gcgcggaacg ccgtcgttgg
     1081 tctgcgccat caaggcgggc agcgcggtgg ctccctggat caggtcgtca taggcgggtt
     1141 ttgccgcata gggcccgtcc tggccgaagc cgaacacgcc ggcatagatc aggcgcggat
     1201 tgatcttcgt gaccacatcg tagccgagct gcagccgcgc catcgcctgc ggacgcacgt
     1261 tatagaccag cacgtcggcc gacgcgatca gccgcaatac cgcgtcgcgc cctgcggctt
     1321 tcttgaggtc gaggcagatg ctgcgcttgc tgcggttggt gttgaggaac accggcccca
     1381 tgccgggatg gcgcgtcggg ccgatctgcc gcgtcacgtc gccgtctgtt gtttcgatct
     1441 tgatgacgtc ggcgccatag tcgccgagca tctgggtggc ataggggccc atcagcacgg
     1501 tcgtcatgtc cacgaccttg atgccctgca atggtcccat ggtacggttt gctcccgatc
     1561 tgttgtcggg gtcaagtaac ggcagggcgc agccgagatc aagcccggtg ggcgtatggg
     1621 ggcatgcgcg gggccgcgcc aggatgggta atttccgcgc agaagacaat gacgcgcagg
     1681 aatttgacgc aacatttgcg gaaaagcctc gacgcacgtc cgctgtctcg acacgtacgc
     1741 ccctcttgtc gaggcgggac gatgtaagaa actgccaatt gaaggcccaa ggaaagcgaa
     1801 ttattttcgc gcgcaggact ggaccaccca aatcagcttg aacggccacg cgaaatcgga
     1861 tttttcgcgc gcgcgatttc ggcggctcaa agggcgcagc gagggaggcg aggtccagag
     1921 aactcaaccg atttgcccgt cgggcagttg gtttcggaaa cttggggctt gcgcactccc
     1981 ctcgccccgt cagcggattt cgggtagctc aaattatgat tgctgcgagc tcaagtcacc
     2041 tttgtgccgg ccaaggcgaa cacgatcgct tcggacaatt gccccagacg gaatggcttc
     2101 gcgagaagct ttgtattggt gcggaagtcg tcgggcacca gcgagggatc cgcgaagccg
     2161 gacataaaga gaatgggcag gtcgggccat ctgcggcgcg cctgacgcgc caagtcaaag
     2221 ccggtcatgc cgccgggcag gagaatatcg gtcagcagca gcg
//
LOCUS       DATNOI010000436         8897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2052,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000436 DATNOI010000000
VERSION     DATNOI010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1589)
                     /locus_tag="VN968_17470"
     CDS             complement(183..1589)
                     /locus_tag="VN968_17470"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXL31058.1"
                     /translation="MTQRFQSTHNGKAPVHTPPNAKRKPRLLDDDHIEALREFADDEP
                     SSLGYYPDSKVFGLTIFLGAKSTVWRFRQQSRTKGKRSSVFKTLGNWPAMDVDEARKQ
                     ALIYSGAVAAGTAAPGKRQAMPFRTAFENYLVHLKAQAEAKGKPPRWWANACKLSEAH
                     IMPQWDGWTLSEMSQNPRAVKTWHAKLSKSIPTTADHCARLIRACYREEARLDRTLNP
                     AASPTSGIRLGKIKVSKAVLDFPDFSAWRKAWDKIENPVHKGYHLAALLTGCRPTELA
                     MIRESDIDLDARRMIIRNAKAGNDISLPITGEIAFALAMAINAPPQTITMKGLRGMKS
                     GEVRVIERKKLHHEIAAPDLVFPGVRQAGHRSGLPVSGNALRHTFRSIAVSLEISEML
                     ISFLMGHSLQGVSARYTNELMIANSQALREAQEKISRRIFELLGLTLGHHDAPLVPDT
                     PTRVEASRKKSSAPARIA"
     gene            1919..2497
                     /locus_tag="VN968_17475"
     CDS             1919..2497
                     /locus_tag="VN968_17475"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HXL31059.1"
                     /translation="MAGRGYTIFDTAVGRCGIAWGDGGVIGVQLPEVREIETRRCLFR
                     LYPDARELPPPLNVETAIDNIAALLRGEGADLSGVTLDLSGIPAFNARVYAFARTIPR
                     GETRTYGEVAACIRASGAVRSVAQAIARNPFVIIVPCHRVLEAGGYADKISPNGGSIS
                     KRRLLSIEGGGSPSSKTLFDVLLPVAPARPQT"
     gene            2507..3347
                     /locus_tag="VN968_17480"
                     /pseudo
     CDS             2507..3347
                     /locus_tag="VN968_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456083.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            3404..3763
                     /locus_tag="VN968_17485"
     CDS             3404..3763
                     /locus_tag="VN968_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL31060.1"
                     /translation="MYDHIGLKVGDLDASVRFYTAALAPLGYVLCSRDDSGAGFGPKG
                     EPALWLYWHKGPAPVGAHVAFRAPDHAAIRKFHADGLKAGGRDNGGAGPRADYSPSYF
                     AAFLIDPDGNNVEAVCT"
     gene            3906..4307
                     /locus_tag="VN968_17490"
     CDS             3906..4307
                     /locus_tag="VN968_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXL31061.1"
                     /translation="MASIHKEIPIDASPADVWAAVRDFGSPHRRLVPGFVLDSRLDGD
                     ARIVTFANGTVARELLVDCDDARRRLVYAAISERIKQHSASVQVTADGEARARLVWIV
                     DVLPNEIAPYIDGQMEQAALAMQKSLGRKTA"
     gene            4423..4875
                     /locus_tag="VN968_17495"
     CDS             4423..4875
                     /locus_tag="VN968_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31062.1"
                     /translation="MTGAGKAVCQAATLLLLFTLTTGPAKAQFGGFGGAGGGDMMTQM
                     APMLEMMKAKMGKRRFGMMMQTVGPMMSQMMEGGGGGFGGMIGGGGIPGGFGGGASFA
                     PGNYAGVGDVMGMLGGNGGSQLMSMIPQLMRMANFGGGHRHHRRHRRR"
     gene            complement(4881..6323)
                     /locus_tag="VN968_17500"
     CDS             complement(4881..6323)
                     /locus_tag="VN968_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXL31063.1"
                     /translation="MPEIAVPEIPVDEEERPLPPPEPLARNALVDGPILRTLLWLAWP
                     NVIALSAGTCVVIAETSYIGRLGVESLAAMALVFPFVILTMTMSGGAMGGGVASAVAR
                     ALGAGDVDRASALAAHALLIGVCFGLTFMLGMLIFGPSLLELLGGHGRVLANAIGYAQ
                     IFFGGAVVPWLMNSMAGILRGTGNMKLPSLMMLTSAACQIVLGGALGLGLGPIPQFGM
                     RGVAAGSLIAYLISIAIMGWYLFSGRARVIPRIRGLRVQWAMFVDILKVGAVSCFSPL
                     QSVLSISIFTHMLAGFGTQILAGYGIGARLEFMLTSVAFAVGIASVPMVGMAIGADRI
                     ARARRIAWTAGLVSFVSVGLIGTLIAIFPDLWVEIFTRDAGVRAASRQYLSIAGPMYA
                     FIGLAMSMYFSSQGAAKVLGPVLAQSARLLFIALGGWWLSMHGATAANFFALAAASMA
                     VLGVLSASSVMLTRWGPKARPMAQARPALS"
     gene            complement(6459..7511)
                     /locus_tag="VN968_17505"
     CDS             complement(6459..7511)
                     /locus_tag="VN968_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31064.1"
                     /translation="MSDGQGDAREAQWLKWRSVAELYHAYFTGLILCVVTRRGTADAA
                     EFVFRVFRRQQQERFLPGLEKLGLGHLPPAVAAAQYHYLSNWIGGVHVEYMHENDRKA
                     WIRYPPPRWIWKGTAICGVSGEVSRAMLRGWHANNGVSLGNPNLGFVCTKQSVDGQDG
                     LEGYYFEYDHALEIGQRLVFARHLEAPMFDPATAPALPVASWPKPRLEKAYRNYAMEY
                     VRTAAPAMVQLFGPEDAGYLLHLTGKLIGMQYFDEIARGFGFGRGTAKEFGAFLRTLV
                     EAQDDVTDASESAGTFEIRQRTWKLMADVTDYHPACTRALAGLFEGLAAGCGRHIPIR
                     MRSSDDGRPPFVWSIG"
     gene            complement(7727..8830)
                     /locus_tag="VN968_17510"
     CDS             complement(7727..8830)
                     /locus_tag="VN968_17510"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HXL31065.1"
                     /translation="MFTVQPGANGILPDRMIAAMADAGLILPAYPFVESQIQPASLDL
                     RLGDVAYRVRASFLPGPGATVAERIDELKLHEIDLTDGAVLETNCVYIVPLLESLALP
                     PEIVASANPKSSTGRLDVFTRVIADGTRRFDMIGAGYHGPLYAEISPKTFPVLLREGS
                     RLSQVRFRIGDAILKADELDALHVLERLVDSKDADLVNGVALSVDLSGENANGFVGYR
                     AKRHTGVVDIDRRGGYAVDEFWEAMPARPDGSLILDPGEFYILASKEAVQVPPDYAAE
                     MVPFDPLVGEFRVHYAGFFDPGFGYAGAGGKGARAVLEVRSREVPFILEHSQIVGRLV
                     YEKMLARPDALYGTRIGSNYQAQGLKLSKHFRV"
BASE COUNT         1663 a         2824 c         2797 g         1613 t
ORIGIN      
        1 aaaattcacc ccagctgctg gtatcgaaga agtgcgattt ggcggattca aaaggatcag
       61 atctaacttc catcgaatta ctgaagctat tttcgccgat tgcgtaaaaa tgtaggcaat
      121 ggttgatcct caaatttgac attcacccag gataaacgtc tttagctttc gcctcccccg
      181 tctcaagcga tgcgggccgg agcggagctc tttttccggc tcgcttcgac ccgggtcggc
      241 gtgtccggca cgagcggcgc atcgtgatgg ccgagcgtga gcccgagcaa ttcgaaaatc
      301 cggcgactga tcttctcttg cgcctctcga agagcttgcg agttcgcgat catcaactcg
      361 tttgtgtacc tcgcggagac gccttgcaga ctgtgcccca tgaggaacga aatcagcatc
      421 tccgaaatct ccagcgagac cgcgatgctg cggaacgtat gccggagcgc gttgcccgag
      481 accggaaggc ccgagcgatg gccagcttga cgaacgcccg gaaagacaag atcgggagcc
      541 gcgatctcgt gatgcagttt ctttcgttcg atcacgcgga cctcgccgga cttcattccg
      601 cgaaggccct tcatcgtgat ggtttgcgga ggcgcgttga tggccatcgc cagcgcgaaa
      661 gcaatctcgc ccgtgatcgg cagcgagata tcgtttccag ctttcgcgtt tcggatgatc
      721 atgcgccgcg cgtcgagatc gatatcgctc tcgcggatca tggcaagctc ggttggccga
      781 cagccggtca acagcgccgc gagatgatag cccttatgaa ccggattttc gatcttgtcc
      841 catgccttgc gccatgccga gaagtcgggg aagtcgagca cggctttcga gaccttgatc
      901 tttccaagcc ttatgccgga ggtcggcgag gccgccgggt tgagcgtgcg atcaagccgc
      961 gcctcttcgc ggtagcaggc ccgaatgagc cgtgcgcaat ggtcggcggt tgttgggatg
     1021 gacttcgaga gcttcgcatg ccacgtcttc accgctcgcg ggttctgcga catttcggag
     1081 agcgtccatc cgtcccattg cggcatgatg tgcgcctcgg atagcttgca ggcgttggcc
     1141 caccatcgcg gcggcttgcc tttggcttcg gcttgcgcct tgagatggac gaggtagttt
     1201 tcaaaagccg tgcggaacgg cattgcttgg cgcttgcccg gggcggcggt gccagcggcg
     1261 accgcgcccg aatagatcag ggcttgcttg cgcgcctcat ccacgtccat cgcaggccag
     1321 ttgccgagcg tcttgaagac cgacgaccgc ttgcccttcg tccggctctg ttgacggaag
     1381 cgccagacgg tagacttagc gccgaggaag atggtcaggc cgaagacctt gctgtcggga
     1441 taatagccca gactgctcgg ctcatcgtcg gcgaactcgc gcaacgcttc gatgtgatcg
     1501 tcgtcaagca agcgcggctt gcgcttcgcg ttcggcggag tgtgaaccgg ggccttgccg
     1561 ttatgcgtac tctgaaatcg ttgtgtcatc gtgatgtcac gatgatgcca tttgactagt
     1621 tcggatgctg ggattttcag ccgccgaggc atcacagata acactagaag cccgcgaaac
     1681 atgggcttta gacaatggtt tctggcgttt ctcgaaacgt ggtaaaaatt cggctgatta
     1741 gattgggaag ctgccgttct gccattgaac tacgcccgcg ggtagcatcc ttgattagcc
     1801 gagacagcgc gatccgccaa gcgccttggc gatgaccccg tcggccttta cccaacgcca
     1861 agttttaagg tgcgcccgtt tgcggcagtg ctatgatccc ggtccggtgg gtggcgtgat
     1921 ggcggggcgt ggatacacca ttttcgatac ggcagtcggc cgatgcggca tcgcatgggg
     1981 cgatggcggc gtcatcggcg tgcagctgcc tgaagtgcgg gagattgaaa cccgccgttg
     2041 cctgtttcgt ctctatcccg atgcccgcga acttcctcca ccgctcaacg tcgaaacggc
     2101 gatcgataat atcgccgcgc tgctgcgcgg cgaaggcgcc gatctctccg gggtcacgct
     2161 cgacctcagc ggcatacccg ccttcaatgc gcgcgtttac gcgttcgcgc ggacgattcc
     2221 gcgcggcgaa acccgcacct atggcgaagt cgccgcttgt atcagggctt cgggggccgt
     2281 gcgctcggtc gcgcaggcga tcgcccgcaa cccgttcgtg atcatcgttc cctgccatcg
     2341 cgtgctcgaa gccggcggct acgccgacaa gatctcgccg aacggcggca gcatctccaa
     2401 acggcggctg ctctcgatcg agggcggagg cagccccagc agcaagaccc tgtttgacgt
     2461 gcttctcccg gttgcgccgg cacgcccgca gacctaaatc tgcggcatgg acagaacgac
     2521 ccttctgcag ggtaaatcca tctcggtacg tgactttcgc tgcacggcgg tgcctggtga
     2581 caagccgttc gtggagcaat acgcgtgcca ttcgatctcg tatgtccgaa aaggcagctt
     2641 cggctgtcac agccgcggcc gcttcttcga gctggtggcg ggatcgatcc tggtcggcca
     2701 tcccggcgac gaatatgtct gcacccacga ccacgtctgc ggcgacgagt gcctgtcgtt
     2761 ctttttaagc ccggaactgg tggaagcgac cggcgaccgc gccgaggcct ggcggatcgg
     2821 cagcgcgccg ccgctgcccg aattgatggt gctcggcgag ctcgcgcagg ccgccgccga
     2881 cggccgcagc gatgtcggcc tcgatgaggt cggacaggtg ttcgccagcc gtttcgtcga
     2941 ggtggtttcc gggcggccgc acaagcccgt ggcgacagcc gcccgcgacc gccgccgcgc
     3001 ggtggaaacg ggcgctttgg atcgatgccc attcgcacca acagatcgat ctggagcagg
     3061 ccgccggtca ggccggcatc agcccgttcc atttcctgcg gctgttctcg ggtgtgttag
     3121 gagttacccc gcatcaatat ctggtgcgct cgcggctacg tcatgcggcg cgcctcctgg
     3181 ccgaccatga catttcgatc acggatgtcg cctacgacgt cggattcggc gacctctcca
     3241 atttcgtgcg caccttccac cgcgccgccg gggtctcgcc gcgcaggttc cgcgagggct
     3301 cgcgcgggat gcgcaagatt ttccaagaac ggctcgccct gcactgacta ggctgatctc
     3361 cggttcgcgc gttttcgagc gcgctcaaca atcggagatc atgatgtacg accatatcgg
     3421 actgaaagtc ggcgatctcg acgccagcgt gcgcttctac acggcggcat tggcgccgct
     3481 cggatacgtc ttgtgttccc gcgacgattc cggtgcaggt tttggcccca agggcgagcc
     3541 ggcgctctgg ctctattggc acaaggggcc cgcccctgtc ggcgcgcatg ttgcgttccg
     3601 cgcgcccgat cacgccgcga tcaggaaatt ccacgccgac ggattgaagg ccggaggccg
     3661 cgacaatggc ggcgccggac cgcgggccga ttacagcccg agctatttcg ccgcgttcct
     3721 gatcgacccc gacggcaaca atgtcgaggc cgtgtgcacg tagcgtcaga gcacctgcac
     3781 ctgcgttcac cgccgtcatt gcgaggagca cagcgacgac gcaatccatc gcgccacgca
     3841 aagaaagaat ggattgcttc gcttcgctcg caatgacggc taactcatta gaaggaccac
     3901 aactcatggc ctccatccac aaggagattc cgatcgatgc ttcgcccgcc gatgtctggg
     3961 ccgcggtgcg cgatttcggc agcccgcatc ggcggctggt accgggattc gtgctcgatt
     4021 cccggctcga tggcgacgcg cggatcgtga cctttgccaa cggcacggtg gcgcgcgaat
     4081 tgttggtcga ttgcgacgac gcgcggcggc ggctggtcta tgccgcgatc agcgagcgga
     4141 taaagcagca cagcgcgtcg gtgcaggtaa cggccgatgg cgaggcgcgc gcgcggctgg
     4201 tctggatcgt cgacgtcctg cccaacgaga tcgctcccta tattgatggc cagatggagc
     4261 aggcggcgct cgcgatgcaa aaatcattgg ggcgcaaaac cgcttagcgc cacgctgcca
     4321 acgtgacctt tctcacagag cgcgctgctg ccagtcgcta gcgtttccag gtgcgtccgt
     4381 tttgcgccgc ttcccgcgga gctacgcacg ggagcgaaaa acatgactgg agccggcaag
     4441 gcggtgtgcc aggcagccac gctgcttttg ctgttcaccc tgacgacagg ccctgcgaag
     4501 gcccagttcg gcggctttgg cggcgcaggc ggcggcgaca tgatgaccca gatggcgccg
     4561 atgctggaga tgatgaaggc gaagatgggc aagcgccgct tcggcatgat gatgcagacc
     4621 gtgggaccga tgatgagcca gatgatggaa ggcggtggcg gcggtttcgg cgggatgatc
     4681 ggcggcggcg gaatccccgg cggatttggc ggcggcgcat cctttgctcc gggaaattac
     4741 gcgggcgtcg gcgacgtcat gggcatgctc ggcggcaatg gcggcagcca gttaatgagc
     4801 atgattccgc agttgatgcg gatggccaat ttcggcggcg gacaccgcca ccaccggcgt
     4861 caccgtcggc gttaatcgcg tcaagacaat gccggccggg cttgcgccat cgggcgggcc
     4921 ttcgggcccc atcgcgtcaa catgacgctc gaggcagaca gcacgccgag caccgccatc
     4981 gatgccgcgg ccagcgcaaa gaagttcgca gcggtggcgc cgtgcatcga cagccaccag
     5041 ccgccgagag cgataaacaa tagccgcgcg ctttgcgcca gcaccggccc caaaaccttg
     5101 gccgcccctt gcgaagaaaa atacatcgac atcgcaagcc cgatgaaggc atacatcggg
     5161 ccggcaatcg acaggtattg ccggctcgcc gcgcgcacgc cggcgtcgcg ggtgaagatc
     5221 tcgacccaga ggtcgggaaa gatcgcaatc agcgtgccga tcaagcctac tgaaacgaac
     5281 gagaccaggc ccgcggtcca ggcaatccgt cgcgcgcgcg caatccggtc ggcgccgatc
     5341 gccatgccga ccatgggcac cgaggcaatc ccgaccgcga acgcaaccga ggtcagcatg
     5401 aattcgagcc gcgcgccgat gccgtaaccg gccagaatct gcgtgccgaa acccgccagc
     5461 atatgggtga agatgctgat gctgagcacc gactgcagcg gcgagaaaca ggacacggcg
     5521 ccgactttga ggatgtcgac gaacatcgcc cattgaacgc gaagtcccct gatcctggga
     5581 atgacgcgcg cccggccgga aaacagatac catcccatga tagctatgct gatcaggtag
     5641 gcgatcaggg agcctgcggc gacgccgcgc atgccgaatt gcggaatcgg gccgaggccg
     5701 aggccgagcg caccaccgag aacgatctga caggcggcgg aggtgagcat catcagcgat
     5761 ggcagcttca tgttgccggt accgcgcaga attcccgcca tcgaattcat cagccagggc
     5821 actaccgcgc cgccgaagaa aatctgcgcg tagccgatgg cgttagccag cacgcggccg
     5881 tggccgccga gcaattcgag cagcgaggga ccgaaaatca gcatgccgag catgaacgtc
     5941 aacccgaagc acacgccgat cagcaatgca tgcgcggcca gcgccgatgc gcggtcgaca
     6001 tcgcctgcac cgagcgcgcg cgcaaccgcc gacgcgacgc cgccgcccat cgcgccgccc
     6061 gacatggtca tggtcaggat cacgaacgga aacaccagcg ccatcgccgc cagcgactcc
     6121 acgccaagcc ggccaatata ggaggtctcc gcgatcacta cgcaggtgcc cgcgctgagc
     6181 gcgatgacgt tgggccaggc cagccacaac agcgtacgca ggatcggccc gtcaaccagc
     6241 gcattcctgg ccaacggctc cggcggcgga agcggacgct cctcttcgtc gacggggatc
     6301 tcggggacgg cgatctcggg cacttcagtt tgtctccctg ggcccgatct cctgccgtgc
     6361 cgtggtgctt cctaccacga tcaagccaga atccacgtgc gccgggcgca atgggggctt
     6421 gccggattgc gggcgaatgc ggccgtaagg ggatcggcct agccgattga ccagacaaac
     6481 ggcggcctgc cgtcatccga cgatctcata cggatcggga tatggcgacc gcagccggcg
     6541 gcaaggcctt cgaacaatcc cgcaagcgcc ctggtgcagg ccggatgata atcggtgaca
     6601 tcggccatca gcttccaagt ccgttgtctg atttcgaacg tgccggccga ctcgctggca
     6661 tccgtaacat cgtcctgcgc ttcgaccagc gtacgcagga aggccccgaa ctctttcgca
     6721 gttcctcgac cgaagccgaa gccgcgtgcg atctcatcaa aatactgcat gccgatcaac
     6781 ttgccggtca gatgcaagag atagccggca tcttcggggc cgaacaattg caccatcgcg
     6841 ggtgcggcgg tccggacata ttccattgcg taattgcgat aggctttttc cagccgcggc
     6901 ttcggccagc ttgcaaccgg caaggccggc gcggtcgctg ggtcgaacat gggggcttcg
     6961 agatgacgcg cgaataccag gcgctgaccg atctcgagcg catgatcgta ttcgaagtag
     7021 taaccttcaa gcccgtcctg gccgtcgacg ctctgcttgg tgcagacgaa accaagattg
     7081 ggattgccga gcgaaacgcc gttgttggcg tgccatccgc gcagcatcgc gcgcgacact
     7141 tcacctgata cgccgcagat cgccgtgccc ttccagatcc agcgcggcgg cggatagcgg
     7201 atccaggcct tgcggtcgtt ctcgtgcatg tattcgacat gcacgccgcc gatccagttc
     7261 gagagatagt gatattgcgc cgctgccacc gccggcggca ggtggccgag cccgagtttt
     7321 tcgaggcccg gcaaaaagcg ctcctgctgc tgacggcgaa atacccggaa cacgaattcg
     7381 gcggcatcgg ccgtcccgcg ccgcgtcacc acgcacagaa tgaggccggt gaaataggca
     7441 tgatagagct cggcgacgct gcgccatttc agccattgcg cctcgcgggc gtcgccttgc
     7501 ccatcgctca tgcttactcc cactccctgt gtttgttggc gcgtgacgcc ggcatcggca
     7561 aagtcggcgc cgcggtaagc gtgccccgga tgccgcgcag cgcgccgccc ttgcggcgtg
     7621 gtgcgctgct gatccggggt ctatgttttt accacattcc cgtgggtccc ggctctgcgg
     7681 cgcaccgccg aagaggcgct gcaccgcgtc cgggacacaa gtgacactaa acccgaaaat
     7741 gcttgctcag cttgaggccc tgcgcctggt agttggagcc gatgcgcgtg ccgtaaaggg
     7801 catcgggtcg cgccagcatc ttttcgtaga ccagccggcc gacgatctgg ctgtgctcca
     7861 ggatgaacgg cacttcgcgc gagcgcactt ccagcaccgc gcgcgccccc ttgccgccgg
     7921 cgccggcata gccgaagccg ggatcgaaaa atcccgcata gtgcacgcgg aattcgccga
     7981 ccagaggatc gaacggcacc atctcggcgg cgtagtccgg cggcacctgg accgcttctt
     8041 tcgaggccag aatataaaac tcgccgggat cgagaatcag gctgccatcg ggccgcgccg
     8101 gcatcgcctc ccagaactca tcgacggcgt agccgccgcg gcggtcgata tcgaccacgc
     8161 cggtgtgacg tttggcgcgg tagccgacga agccattggc gttctcgccg ctcagatcga
     8221 cgctcagcgc gacgccattg acgagatcgg cgtccttgct atcgaccagc cgttccagca
     8281 catgcagcgc gtcgagttcg tcggccttga ggatggcgtc gccgatgcga aagcgcacct
     8341 ggctgagccg cgatccctcg cgcagcagca ccggaaatgt tttcggactg atctcggcat
     8401 agagcggacc gtggtagcca gcgccgatca tgtcgaaccg tcgggtgccg tcggcgatca
     8461 ctcgggtgaa cacatcgagc cgcccggtgg aacttttcgg attggcggag gcgacgattt
     8521 ccggcggcag cgcaaggctc tccagcagcg gcacgatgta gacgcagttg gtctccagca
     8581 ccgcgccatc ggtcaaatcg atttcgtgca gcttcaattc atcgatgcgc tccgccaccg
     8641 tcgcccctgg accggggaga aaactcgcgc gcacccgata ggcgacgtcg cccagacgca
     8701 gatcgaggct cgccggctgg atctggcttt cgacgaacgg gtaggccggc aggatcaggc
     8761 cggcgtccgc catcgccgcg atcatgcggt cgggcagaat gccattggca ccgggttgaa
     8821 ccgtgaacac ttgatggccc tcgggggttt caccctacac aaaccctgaa aatatgcact
     8881 tttaccggtt atccgat
//
LOCUS       DATNOI010000437         5159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1445213,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000437 DATNOI010000000
VERSION     DATNOI010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445213"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="VN968_17515"
     CDS             <1..1506
                     /locus_tag="VN968_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HXL31066.1"
                     /translation="NLFSRLFDALDDPARLAIETTDGRRIDYGELISRAGQMANVLTS
                     RGVKPGDRVAAQTEKSVPGLVLYLAAVRAGAVYLPLNTAYTLNELEYFITDAEPSLVV
                     CDPAKADGIGAIAAKVGAKVETLGADGKGSLTDAAAKAPAPFETVARADDDLAAILYT
                     SGTTGRSKGAMLTHDNLASNSLTLVDYWRFTNKDVLIHALPIYHTHGLFVASNVTLFA
                     RASMIFLPKFDPELIIKLMARATVLMGVPTFYTRLLQSPALSHESTRHMRLFVSGSAP
                     LLADTHREWAARTGHAVLERYGMTETNMNTSNPYDGQRVPGAVGHALPGVTVRVTDPE
                     TGKELARESIGMIEVKGPNVFKGYWRMPEKTKAEFRDDGFFITGDLGKIDANGYVHIL
                     GRGKDLVISGGFNVYPKEIESEIDAMPGVIESAVIGVPHADFGEGVTAVVVCDKSARI
                     DEASVLKALDGRLAKFKMPKRVIVVDELPRNAMGKVQKNILRDIYSKIYAK"
     gene            complement(1494..1748)
                     /locus_tag="VN968_17520"
     CDS             complement(1494..1748)
                     /locus_tag="VN968_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31067.1"
                     /translation="MKRREFIALAGGEAGSFVEWVKRSEPTNFLASELWSWWHGAKRV
                     FAHPTKLSWHRHCEEQSDEAIHLSFSGLLRCARNDVAYFA"
     gene            complement(1745..2473)
                     /locus_tag="VN968_17525"
     CDS             complement(1745..2473)
                     /locus_tag="VN968_17525"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31068.1"
                     /translation="MVSLAPDVILAAGNAPVERLLQATRTVPIVFVIVLDPVGSGIVD
                     SLSQPGGNATGFMQFEYSLTAKWLELLKQVAPGVTRAAVLRDSTVAAGIGQFAVIQSV
                     APSLGVEVSPINGRDASEIERTIAAFARASNGGLIVTSSALAGVHRDLIVRLAASHKL
                     PAVYSNRASVTGGGLISYGADLVDQYRRAAGYVDRILKGEKPADLPVQAPTKYELVIN
                     LKTAKALGLDVSPSLLARADEVIE"
     gene            complement(2483..2734)
                     /locus_tag="VN968_17530"
     CDS             complement(2483..2734)
                     /locus_tag="VN968_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31069.1"
                     /translation="MKRREFMALLGGVAASLPLASRAQQPDRMRRIGVLVGASGDGMD
                     AQVRHATFLQGLQQLGWTDGQNVRIDSRWGAANADDIRK"
     gene            complement(2731..3711)
                     /locus_tag="VN968_17535"
     CDS             complement(2731..3711)
                     /locus_tag="VN968_17535"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31070.1"
                     /translation="MRRREVILALGGAAASTPFAARAQRPATPPTIGFLGATTSATAN
                     QWVAAFVARLKELGWVEGRTVMIEYRWAEAHSERYAEIANELVDHKVDVIVTWASAPV
                     LAAKHATTVVPIVFAAQMDPVGAGVVASLARPGGNVTGLSLQQVDTAGKRLELLREFV
                     PHLGRLAIMANIDAAGAILERREIQATAHTLGLEVANLEIRQTDDIAAAIEALKGHAD
                     ALYVATDPLVFTNRVRINSLAQDARLPTIYGSREYVEAGGLMSYGPSYTDLFRRAADY
                     VDKILRGTKPGDIPVEQPTRFELIVNLKTAKALGLNVSPTLLARADEVIE"
     gene            complement(3708..4649)
                     /locus_tag="VN968_17540"
     CDS             complement(3708..4649)
                     /locus_tag="VN968_17540"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31071.1"
                     /translation="MAWPLSVRAQQPAGRVYRVGYLAIASRAISLHEAFEEGLRKLGY
                     RVGENVVIEYRFADGEMERLPALAADLVRLGVDIIVSGTNVTTVAAMKATTTIPIVMT
                     NSAEPVSAGLVASLARPGGNVTGFSSEPGDEIYGKRLEFLKETLPNLSRVGILWNPDF
                     APNQDRLTSIREAAPALGLTLVPAEARGLDALEQAFATMVRERAQVLVVLSDGVLFNC
                     RGQIGVMALGNRLPAISAVREYAEAGLLLSYGIDLPDQFRRTAVFVDKIFKGAKPADL
                     PVEQPTKFELLVNLKTAKALGLDVPPTLLARADEVIE"
     gene            complement(4685..>5159)
                     /locus_tag="VN968_17545"
     CDS             complement(4685..>5159)
                     /locus_tag="VN968_17545"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31072.1"
                     /translation="NPDVALNRSRQTSMGETARTLGLTLVPAEARGLDALEPAFATMV
                     RERAQAFVMQGDSVLFTYRGQIAEMALRNRLPAVSLQKEHAEAGFLLTYGADIPDLYR
                     RLAGFVDKIFKGAKPADLPVEQPTKFELVINLKTAKTLGLTIPPTLLARADEVIE"
BASE COUNT          977 a         1659 c         1576 g          947 t
ORIGIN      
        1 aatctgtttt cccgcctgtt cgacgccctc gacgatccgg cccggctcgc gatcgaaacc
       61 accgacggac gacgcatcga ttatggcgag ctgatttctc gcgccgggca gatggcgaat
      121 gtgctgacgt cgcggggcgt caagcccggc gaccgcgttg ccgcgcagac cgaaaagtcc
      181 gtcccggggc tggttttgta tctcgccgcg gtgcgggcgg gtgcggtgta tcttccgctc
      241 aacaccgcct atacgctgaa cgaactcgaa tactttatca ccgacgccga accgtcgctg
      301 gtggtgtgcg atcccgccaa ggcggatggc atcggagcga ttgcggcgaa ggttggcgcc
      361 aaggtcgaga cgctgggcgc cgacggcaag ggatcgctga ctgatgctgc cgcgaaggcg
      421 cctgcgccat tcgaaacagt tgcgcgcgcc gacgatgatc tcgccgccat cctctatacc
      481 tcaggcacca caggccgttc caaaggcgcg atgctgacgc acgacaatct cgcgtcgaac
      541 tcgctgacgc tggtcgacta ttggcgcttc accaacaagg acgtgctgat ccacgcgctg
      601 ccgatctatc acacccacgg actgtttgtg gcgagcaatg tcacgctgtt cgcccgcgcc
      661 tcgatgattt ttctgccgaa gttcgatccc gagctcatca tcaagctgat ggcgcgcgca
      721 accgtgctga tgggcgtgcc gacgttctat acgaggctgt tgcaaagccc ggcgctcagc
      781 catgaatcca ccaggcacat gcggctgttc gtttccggat cggcgccgct attggccgat
      841 acccatcgcg aatgggccgc gcgcaccggc cacgcagtgc tcgagcgcta cggcatgaca
      901 gaaaccaaca tgaacacctc caatccatat gacggccagc gcgtccccgg cgcggtcggc
      961 catgcgctgc ccggcgtcac tgtgcgcgtc accgatccgg aaaccggaaa ggagcttgcc
     1021 cgcgaatcga tcggcatgat cgaggtcaag ggcccgaacg tcttcaaggg ctattggcgg
     1081 atgccggaga agaccaaagc cgaattccgc gacgacggat tcttcatcac cggcgatctc
     1141 ggcaagatcg acgctaacgg ctacgtccac attctcggcc gcggcaagga tctggtgatc
     1201 tccggcggct tcaacgtcta tccgaaggag atcgagagcg agatcgacgc catgccgggc
     1261 gtgatcgaat ccgccgtgat cggcgtgccg catgccgatt tcggcgaggg tgtcaccgct
     1321 gtcgtggtgt gcgacaagag cgcccgcatc gatgaagcct cggtgctgaa ggcgctggac
     1381 ggacgccttg caaaattcaa gatgccgaag cgggtgatcg tggtcgacga attgccgcgc
     1441 aacgccatgg gcaaggtgca gaaaaatatc ttgcgcgata tttattcgaa aatttacgcg
     1501 aagtaagcaa cgtcattgcg agcgcagcga agcaatccag aaaaagaaag gtggattgct
     1561 tcgtcgcttt gctcctcgca atgacggtgc caagagagct tcgttgggtg ggcaaagacg
     1621 cgcttcgcgc cgtgccacca actccagagt tcagaggcga ggaagttcgt gggctcgctt
     1681 cgcttgaccc actctacaaa actgccggcc tccccgccgg ccagcgcgat gaactcccgc
     1741 cgcttcattc gatcacctcg tcggcgcggg cgagtagcga cggcgacacg tcaagcccaa
     1801 gcgccttggc ggtcttgagg ttgatgacca gttcgtactt ggttggcgcc tgcaccggca
     1861 ggtcggctgg cttctcaccc ttgaggatgc ggtcgacgta gccggccgcg cgacggtatt
     1921 gatcgaccag atcggcacca taggagatca ggccgccgcc agtgacggag gcgcggttgg
     1981 agtagaccgc aggtagtttg tggctggccg caagcctgac tatcaggtct cgatgaactc
     2041 ccgccaacgc gctcgatgtc acgatcaggc caccattgga ggcgcgcgcg aaggccgcga
     2101 tggtgcgctc gatctccgat gcgtcgcgcc cgttgatcgg gctcacctcc acgccgagcg
     2161 acggcgccac ggactggatg acggcgaact ggccgatccc ggcggctact gtggaatccc
     2221 gaagcaccgc cgcgcgcgtc acgcctggtg caacctgctt gagcagttcc agccatttcg
     2281 cggtcaaact gtattcgaac tgcatgaagc cggtagcgtt gccgccgggc tgcgacaaac
     2341 tatcgacgat accggagccg actggatcga ggacgatcac gaacacgatc ggcacggtgc
     2401 gcgtcgcctg tagcagccgc tccacgggcg cgttgccagc cgctagaata acgtccggcg
     2461 cgagcgagac caattccgcc gcctatttgc ggatgtcatc ggcattggca gcgccccagc
     2521 ggctgtcgat ccgcacgttt tggccatcgg tccagcccaa ttgctgcagc ccctgcagga
     2581 acgtcgcgtg gcggacctgt gcgtccatac catccccgct tgcgcccacc agcacgccga
     2641 tgcggcgcat gcgatcgggc tgctgcgcac gcgatgcaag cggaagactc gcggccacgc
     2701 cgccaagcaa tgccataaac tcgcgccgct tcattcgatc acctcgtcgg cgcgggcgag
     2761 cagcgtcggt gacacgttaa gtccaagggc ctttgcggtc ttgagattga cgatcaattc
     2821 gaacctggtc ggctgttcga cggggatatc gcccggcttg gtgccgcgca aaattttatc
     2881 gacataatcg gcggcgcgcc ggaacagatc cgtgtagctg ggtccatagg acatcaggcc
     2941 tccggcttcg acatattcgc ggctgccgta aatcgtgggc agccgcgcgt cctgcgccaa
     3001 gctgttgatt cgaacacgat tggtgaatac gaggggatcg gtcgcgacgt aaagcgcgtc
     3061 cgcgtgtccc ttcagcgcct cgatggcagc cgcgatatca tcggtctgcc ggatttccag
     3121 gttggcgact tcgaggccca aggtgtgggc ggtagcctgg atctcgcgcc tctccagtat
     3181 ggcaccggca gcatcgatat tggccatgat cgccagccgg ccaagatggg ggacaaactc
     3241 gcgcaaaagc tcgagacgct tgccagccgt atcgacctgc tgcagcgaca ggcctgtcac
     3301 gttaccgccc ggccgcgcca ggctcgcgac gacgccggcg ccgaccgggt ccatctgagc
     3361 ggcaaacacg atcggaacaa ctgtggtggc gtgcttcgcc gcgagaaccg gcgccgacgc
     3421 ccatgtaacg atgacatcga ctttgtgatc gacaagttca ttcgcgatct cggcatagcg
     3481 ttcgctgtgt gcttcggccc agcgatactc gatcataacc gtgcgacctt cgacccaacc
     3541 gagttccttt agccgcgcga cgaacgcggc tacccattgg ttcgcggtcg cggacgtcgt
     3601 cgcgcccaga aatccgatcg tcggcggcgt ggccggccgc tgcgcgcgcg ccgcgaatgg
     3661 cgtactcgcc gccgcgccgc cgagagccaa gattacctcg cgccttctca ttcgatcacc
     3721 tcgtcggcgc gggcgagcag cgtcggcggc acgtcgaggc cgagcgcctt tgcggtcttg
     3781 aggttcacca gcagctcgaa cttggtcggc tgctcgaccg gcaggtcggc aggcttcgca
     3841 cccttgaaaa tcttgtcgac gaagacggcg gtccgacgaa attgatctgg caaatcgatc
     3901 ccgtagctca gaagcaagcc cgcctccgcg tattctctca ccgcggagat tgccggcagc
     3961 cgatttccga gggccatgac gccaatctgg ccacgacaat tgaacagcac accatcactc
     4021 aatacgacga gcacctgcgc gcgttccctc accatcgtcg cgaacgcttg ctcaagtgcg
     4081 tccagtccgc gtgcctcggc cgggacaagc gtcaacccca gcgccggggc agcctccctt
     4141 atcgacgtca gccggtcctg attgggggca aagtccggat tccacagaat gcccacacgc
     4201 gagagattcg gtagtgtttc cttcagaaat tcgagccgct tgccgtagat ctcgtcgcca
     4261 ggttccgagc tgaacccggt gacgttgccg cccggacgcg ccagactggc gaccaatccg
     4321 gcactgaccg gctcggcact gttggtcatg acgatcggga tcgtcgtggt cgccttcatg
     4381 gccgcaaccg tggtcacatt ggtcccggaa acgatgatgt ccacgccgag tcggaccagg
     4441 tctgccgcaa gcgcgggcag ccgttccatc tcaccgtcgg caaagcggta ttcgatgacg
     4501 acattctcgc caacgcggta gccgagcttt cgcaggccct cctcgaacgc ttcatggagg
     4561 gatatcgccc gcgacgcgat cgcgaggtag cccacccggt agacccgccc tgccggttgc
     4621 tgcgcccgca ccgatagcgg ccatgccaat gtggtgccga tccctgcgat gaacgcccgc
     4681 cttctcattc gatcacctcg tcggcgcggg ccagcagtgt gggagggatg gtgaggccga
     4741 gtgtcttggc ggtcttcaaa ttgatcacca gctcgaattt ggtcggttgt tcgaccggca
     4801 ggtcggcagg cttcgcaccc ttgaaaatct tgtcgacaaa gccggctaac cgacgataaa
     4861 gatctggaat atcggccccg taggtcagaa ggaagcccgc ctccgcatgc tccttctgga
     4921 gagagacggc tggcaaccgg ttccttaaag ccatttctgc gatctggccg cgataggtga
     4981 acagcacact atccccctgc atgacgaacg cctgcgcgcg ctccctcacc atcgtcgcga
     5041 acgctggttc aagtgcgtcc agaccgcggg cttcggccgg gacaagggtc aaccctagtg
     5101 tccgggcggt ctcccccatc gacgtctgcc ggctccgatt gagtgcaacg tccggattg
//
LOCUS       DATNOI010000438         3569 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1611773,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000438 DATNOI010000000
VERSION     DATNOI010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611773"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            918..2072
                     /locus_tag="VN968_17550"
     CDS             918..2072
                     /locus_tag="VN968_17550"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXL31073.1"
                     /translation="MHKGRVVFGAMDEVVFGRPAAEAVVGQMDRLGAARAILMVSGTL
                     NRETDEIEKIRQRLGSRCVATFDAMPPHTPREAVIAAAAQARAVNADLIVTVGGGSIT
                     DGAKAVQLCLANHISTVEGIDQIRAHKGVAPDMKAPSVRQISVPTTIAGGEFSAIAGV
                     TNQQTRVKEMLRHDLVMPRAAILDPAVTVHTPEWLFLSTGIRAVDHCVEGLCSREAHP
                     YVDAQAVKGLSMLTQALPRVRENPGDLDARMDCQIGTWLSMGPLSAGVPMGASHGIGY
                     VLGAVYNVPHGYTSCVMLPSVMRWNKPDNAERQELVSAAMGHPGEDAAEVLDWFIRDL
                     GMPRSLHEIKIGPEHFERIAEQAMATPWIPRNPRKIDGPAQVREILVLAA"
     gene            2089..>3569
                     /locus_tag="VN968_17555"
     CDS             2089..>3569
                     /locus_tag="VN968_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXL31074.1"
                     /translation="MYTGNHAYLRPLQPAFIMANSGEAVTYAELEARSNRLVHLLRNR
                     GLKRLDHYAIFMENNSRYLEACGAGERSGLYYTCVNSYLTPSELAYIVNNSESRVLIT
                     SVAKLDVAREALKECPKVELCIVADGPGESERIVGLQEVTAGLPKTPIGDEYIGTAML
                     YSSGTTGRPKGILRPLPEQPPQQQLPMFDFLQKLWQYREGMIYLSPAPLYHAAPQAAV
                     NLTIRSGGTAIIMESFDPERYLELIEKWGVTHSQLVPTMFSRMLKLPEAARKRHDLSS
                     LEIAIHAAAPCPGLVKADMIEWWGPIIHEYYGATEGLGFTACNSAEWLAHRGTVGKVL
                     LGDLHILDENMQPCPVGTPGTVWFKTAAPFEYFKDPTKTKEARSPDGSMSTVGDVGYV
                     DSDGFLYLTDRATFMIISGGVNIYPQECENLLITHPKIADAAVFGVPNADLGEEVKAV
                     VQPMPGILPGPDLAQELILFCSQSLSRQKVPRSIDFEAELPRLP"
BASE COUNT          698 a         1116 c         1117 g          638 t
ORIGIN      
        1 caaaagagcc cgatcgccgg ggagagcacg aaggaaaccg ttaaaaccat tgcgtgcggg
       61 aacgtcgggt gattccgggg tactcgttgt tactcgtgtg cgttctacca ctaccaagtg
      121 cacacgaggc cgcgggtgcc aagggcatcc ggcgttcccc acgccctctt tgggcgtaag
      181 atttatcaac ggctcgggcg catcgcgcgg cgaggtcgta aacgtgtgtc tggagtcgag
      241 atgtcaacac tcgtcatgcc cgggcaaaag cgcgaagcgc gtcttcgcgc tagatgaccc
      301 gggcatccat caatcttcgc gagaccattt ttcgaagaag atggattgcc gggcataggc
      361 gagcggaagc gacgccgtcc ttcggacggc tatgcccggc aatgacggag tgagttgatg
      421 gcggctgttt gaaattgaat ccgaggtaat tcgttgaaag cgatcgtgcc acactttcag
      481 tcgtcatcgc gcgaaagagc ctgtgaagaa taccgacctc atcctgagga gcgcgccctc
      541 gcgcgtctcg aaggatggcc gcgagtccct gctttgcgtc catccttcga gacgcttgct
      601 tcgcaagctc ctcaggatga ggtccgtatc gttcacaacc tcgaaagcgg gcgatccagt
      661 attccgaggc atcaatgatg gaatcgaaag gccgcagcgt actggatacc ccgctgaagc
      721 ctgtcagcgg gctcgccgaa ggcgagaccc ggtggcgggg tatgacggcg gtgttttggg
      781 cgacaccttc cgtagcccgg acggaaccac ccgcgcgaat gtgcgcccga ggacatttta
      841 tggttcccct caccgcagac tccgctagat tatcgtcaaa ggccgtaaca aggccaaaac
      901 gtccgggagg gacaagcgtg cacaaggggc gtgtcgtatt cggcgccatg gacgaggtgg
      961 ttttcgggcg ccccgccgcc gaggcggtgg tcgggcagat ggaccgtctg ggtgcggcgc
     1021 gcgcgattct gatggtcagc ggcaccctca accgcgagac cgacgagatc gagaaaatcc
     1081 gccagcgcct tggatcccgc tgcgtggcga ccttcgatgc gatgccgccg catacgccac
     1141 gcgaggccgt cattgccgcc gccgcgcagg cccgcgccgt caacgccgat ctcatcgtca
     1201 cggtcggcgg aggctcgatc accgacggcg ccaaggcggt gcagctttgc ctcgccaacc
     1261 atatcagcac cgtcgaaggc atcgaccaga tccgggcgca caagggcgtc gcgccggaca
     1321 tgaaggcgcc atcggtgcgc cagatcagcg tcccgacgac gattgccggc ggcgagttca
     1381 gcgccatcgc cggcgtcacc aaccagcaga ccagggtcaa ggagatgctg cgccatgatc
     1441 tggtgatgcc gcgcgcagcg atcctcgatc ccgcggtgac cgtgcatacg cccgaatggc
     1501 tgtttctttc gaccggcatc cgcgccgtcg atcattgtgt cgaaggcctg tgctcgcggg
     1561 aggcccatcc ctacgtcgac gcccaggcgg tgaaggggct ttcgatgctg acgcaggcgt
     1621 tgccgcgcgt gagggaaaac cccggtgatc tcgacgcccg gatggattgc cagatcggca
     1681 cctggctgtc gatgggaccg ttgtccgcgg gtgtgccgat gggagccagc cacggcatcg
     1741 gttacgtgct cggcgccgtg tataacgtgc cccacggata cacctcctgc gtgatgctgc
     1801 cgtcggtgat gcgctggaac aaacctgaca acgccgagcg gcaggaactg gtttccgcgg
     1861 cgatgggaca tcccggcgag gatgccgccg aggtgttgga ctggttcatc cgcgaccttg
     1921 gaatgccgcg cagcctgcac gaaatcaaaa tcggtccgga gcatttcgag cggatcgcgg
     1981 agcaggccat ggcgacgccg tggattccgc gcaacccgcg caagatcgat ggcccggcgc
     2041 aggtgcgcga gatccttgtt ctggccgcat aggagcgtcg gagacatcat gtacaccggc
     2101 aaccatgctt atctccgtcc gcttcagccc gccttcatca tggcgaattc cggcgaggcg
     2161 gtgacctatg ccgagctgga agcgcgcagc aatcgtttgg tgcatttgct tcgcaatcgc
     2221 ggtctcaagc ggctcgacca ctatgcgatc ttcatggaaa acaacagccg ctacctggag
     2281 gcctgcggcg ccggcgagag gtccggactt tattacacct gcgtcaattc gtatctgaca
     2341 ccaagtgaac tcgcctacat cgtcaacaac agcgaatccc gcgtcctgat cacgtcagtg
     2401 gcaaagcttg acgtcgcgcg cgaggcgctc aaggagtgcc ccaaggtcga actctgcatc
     2461 gttgccgatg gcccgggaga gagcgaacgc atcgtcggcc tgcaagaggt cacggctggg
     2521 ctgcccaaga cgcccatcgg cgacgagtac atcggcaccg cgatgcttta ttcgtccggc
     2581 acgaccggcc gaccgaaagg catcctgcgc ccacttccgg agcaaccgcc gcaacagcag
     2641 ctcccgatgt tcgacttcct gcaaaaactc tggcagtacc gcgagggcat gatctatctg
     2701 tcgccggcgc cgctctatca tgcggcaccg caggccgccg tcaatcttac gatccgcagc
     2761 ggcggcaccg ccatcatcat ggaaagcttc gatccggagc gctatctcga gctgatcgag
     2821 aaatggggcg tgacccacag ccagctggtg ccgacgatgt tctcgcgcat gctgaagctg
     2881 ccggaggccg cgcggaaacg ccacgacctg tcatccctgg agatcgcgat ccacgccgcg
     2941 gcgccctgtc ccgggttggt gaaggcagac atgatcgagt ggtggggacc gatcatccac
     3001 gaatattacg gcgccaccga aggccttgga tttaccgcct gcaacagcgc cgaatggctg
     3061 gcgcaccggg gaaccgtcgg caaggtcctg ctcggcgacc ttcacattct cgatgaaaat
     3121 atgcagccct gcccggtcgg cacgccgggc acggtctggt tcaagaccgc ggcgcccttt
     3181 gaatatttca aggatccaac caagacgaaa gaggcccgct caccggacgg cagcatgagc
     3241 acggtcggcg acgtcggcta tgtcgacagc gacggttttc tctatctgac cgatcgcgcg
     3301 accttcatga tcatctcagg cggcgtcaac atctatccgc aggagtgcga gaatctgctg
     3361 atcacccatc ccaagatcgc ggacgccgcc gtgttcggcg ttcccaatgc cgatcttggc
     3421 gaggaagtaa aagcggtggt gcagccgatg ccggggattc tgccgggacc ggatctcgcc
     3481 caggagctga tattgttttg cagccagtcc ctgtcgcgcc agaaggtgcc gcgctcgatc
     3541 gacttcgagg ccgagttgcc gcggcttcc
//
LOCUS       DATNOI010000439         6526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_659540,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000439 DATNOI010000000
VERSION     DATNOI010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659540"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..830)
                     /locus_tag="VN968_17560"
     CDS             complement(93..830)
                     /locus_tag="VN968_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXL31075.1"
                     /translation="MSLDDLPPGAPQPPAREAEAVVRRVDRASPYPDKITRAEELRLQ
                     LADEIVRGALPPGAALDETDIARRFNVSRTPVREALRQLAASGLVDARAHRGAVVARP
                     SIERLTGMFEAMAELEALCAGLAAERMQPSERHRLEAIHEELRVLSYAGNPDRFHEVN
                     ERFHNAIYAGSQNGYIAEMTLATRVRVQPFRRAQFRNLGRLAKSHAEHDRVVVAILRG
                     DKVGAAVAMRAHIELVRDEYELYAVSV"
     gene            complement(827..1240)
                     /gene="hpxZ"
                     /locus_tag="VN968_17565"
     CDS             complement(827..1240)
                     /gene="hpxZ"
                     /locus_tag="VN968_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalurate catabolism protein HpxZ"
                     /protein_id="HXL31076.1"
                     /translation="MEVDLPDVLAEVTGQFARYEAALVGNDVTVLDELFRADARTLRY
                     GIGENLYGYDAITAFRAARSPVGLMRRTERTVITTYGRDTAVASTLFYRDSTPGRVGR
                     QMQTWVRFPEGWKIVAAHVSVIDEPGSSKVMNQKA"
     gene            complement(1242..2639)
                     /locus_tag="VN968_17570"
     CDS             complement(1242..2639)
                     /locus_tag="VN968_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtzE family amidohydrolase"
                     /protein_id="HXL31077.1"
                     /translation="MIANPDDLSALRIAQAVTSRKISALGVTEAALARIAKHDPVLNA
                     FTDVTADRARAKARAVDAAVTAGQNPGPLAGVPYAVKNLFDVQGLPTRAGSKINRDLA
                     PSPRDATLIERLDAAGAVLVGALNMGEYAYDFTGENVHDGPSRNPHDVTRMTGGSSGG
                     SGGAVGGGLVPLALGSDTNGSIRVPSSFCGIFGLKPTYGRLSRARSFPFVASFDHLGP
                     FARSVDDLALAYDAMQGPDPEDAACTTRPAEPVTPLLAQDIGSVRIAIAGGYFQHNVF
                     PEAVEAVARVAKALGATRAIEIPEAARARAAAYVISTTEGASLHLDRLRQRPGDFDPA
                     VRDRLLAGAMIPAPLVDRAQKFRRWYRASVLELFKSVDVIIAPATPCVAPKLGQATFV
                     LDGVELPVRANIGIHTQPISFIGLPVVAVPVPLQPMPIGVQIIAAPWREDIALRVAYA
                     LERAGVVSAARPRGL"
     gene            complement(2647..2829)
                     /locus_tag="VN968_17575"
     CDS             complement(2647..2829)
                     /locus_tag="VN968_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4089 domain-containing protein"
                     /protein_id="HXL31078.1"
                     /translation="MAADPLDAYIDAVSNALALPIEEAWKPAVKANLEVSLRLARLVD
                     EFVLPDETEPASVFAA"
     gene            complement(2849..4636)
                     /locus_tag="VN968_17580"
     CDS             complement(2849..4636)
                     /locus_tag="VN968_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31079.1"
                     /translation="MTAQPLLDVEDLTVEFATRRGIVKAVQHVNISVAKGETLGIVGE
                     SGSGKSVTSYAVMRILDRAGKIAEGSVMFSGVDVKSATENQMRDLRGREISMIFQSPR
                     LALNPIRKIGRQIEDVLRQHVQSAASDRGEKAIEALEQVKIARPRERYHAYPFELSGG
                     MCQRVVIALAPACNPQLLIADEPTTGLDVTTQKAVMDLIVELTRRRGMSTILITHDLG
                     LAAAYCDRVVVMEKGRVVETALSADIFARPEHAYTRKLMRATPRLGVSLRDLLPEEEA
                     AAVMAGLDPTIHPSSQDSSSKAMDPRVKPGGDGSASGDSGAKPLLLVEKLVKEYPRQG
                     ATATLGKLFGRKPPVETEQFRAVDGISFSVGHGESVGLVGESGCGKSTTSMMVMRLLD
                     QTSGRIVFDGDEISAILPNAFARLPLRKSIQMVFQDPTDSLNPRFTAARAIVDPIMQL
                     GDIKGRDALRARCEELAGLVGLPVNLLDRFPHQLSGGQKARVGIARAIALHPKLVILD
                     EPTAALDVSVQAVVLNLLHDLKQSMGMSYLFVSHDLNVVRLLCDRVIVMRTGRIVEQG
                     PSERVLGDPQDTYTRELLTAIPHPPLPVH"
     gene            complement(4778..5680)
                     /locus_tag="VN968_17585"
     CDS             complement(4778..5680)
                     /locus_tag="VN968_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31080.1"
                     /translation="MSEASLTAAPAAEVQPKPPANSIFRHARYVVAENPVTGLSFGLF
                     VIIALCALIGPYIAPYDPLASDTSATLQSPSLHHWFGTDLLGRDIFSRVLVATRLDFV
                     IAVASVALVLVMGGLAGIAAGFFGGWTDRIVGRISDTIMAFPLFVLAMGIVAALGNTV
                     TNIVIATAIINFPLYVRVARAEANVRRDAGFVQAARLSGNSDFRIVMVHILPNIMPIM
                     MVQISLTMGYAILNAAGLSFIGLGVKPPTPEWGIMVAEGATNIISGEWWVAFFPGGAL
                     MIAVFCFNLLGDGLRDVVDPQRRT"
     gene            complement(5677..>6526)
                     /locus_tag="VN968_17590"
     CDS             complement(5677..>6526)
                     /locus_tag="VN968_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31081.1"
                     /translation="GLDRPLPDQFAHYVNDLAHGNFGNSLSTGRPVATEITSRLPASA
                     ELTLLGLFLAIVIAVPLGIFAAVKQGSLIDHLCRIVATAGVSLPVFFTGLLLVYIFYF
                     KLGWSPAPIGRLDAFATAPPDITGFYLIDSLVTGNFETFRASLSQLILPAATLAIFSL
                     APITRMTRASMLAVLSSEFIRTARASGLDDRTVILTYGFRNAMLPVVTTLGMVFSFLL
                     GANVLVEKVFAWPGIGSYAVEALLQSDFAPVQGFVLTMAVLYVALNLLIDMLYSVIDP
                     RVRLEA"
BASE COUNT         1248 a         2126 c         2097 g         1055 t
ORIGIN      
        1 gcgcgcttct tagcgcgagc ccggaatcca taaccacgac cgggagtatg gattccgggc
       61 ctgcgccaag tggcgcatcc cggaatgaaa gcttacaccg acaccgcgta gagctcgtat
      121 tcgtcgcgca ccagttcgat atgcgcgcgc atcgcgacgg cggcgccgac cttgtcgccg
      181 cgcaggatcg ccaccaccac gcggtcgtgc tcggcatgcg atttcgccag gcgcccgaga
      241 ttgcggaatt gcgcgcgacg gaacggctgc acccgcaccc gcgtcgccag cgtcatttcg
      301 gcgatatagc cgttttgcga gccggcatag atcgcgttgt gaaaccgctc gttgacttcg
      361 tgaaaccgat ccggattgcc ggcatagctc aacacccgca gttcctcgtg gatggcttcc
      421 agccggtgcc gctccgaagg ctgcatgcgc tccgcggcga gtcccgcgca cagcgcttcc
      481 agctccgcca tcgcctcgaa catcccggtc agccgctcga tcgacggccg cgccaccacc
      541 gcgccgcgat gggcccgcgc atcgaccaga cctgaggccg caagctggcg caatgcctcg
      601 cggaccggcg tgcgcgacac gttgaagcgc cgcgcaatgt cggtctcgtc gagcgctgcg
      661 cccggcggca gcgcgccacg cacgatttca tcggcaagct gcagccgcag ttcctccgcg
      721 cgcgtgatct tgtccggata gggcgaggcg cggtcgacgc gccgaaccac cgcctcggct
      781 tcacgggccg gaggttgcgg agcgccaggg ggaagatcgt cgagactcat gccttttgat
      841 tcataacttt ggaagatccg ggttcatcga tgacgctgac atgggcggcg acgattttcc
      901 agccttccgg gaatctaacc catgtctgca tctgccgtcc aactctacct ggcgtagagt
      961 cgcgataaaa cagtgtcgag gctacggccg tgtcgcggcc ataggtcgtg atcacagtcc
     1021 gttccgtccg gcgcatcagg cccaccggcg atcgcgccgc gcgaaacgcc gtgatcgcgt
     1081 catagccata gaggttttcg ccgatcccgt agcgcagcgt gcgggcatcg gcgcggaaca
     1141 actcgtcgag cacggtgacg tcgtttccga ccagcgcggc ctcatagcgc gcgaattggc
     1201 cggtcacctc ggcgagcacg tcgggcagat cgacttccat gtcataatcc tcgtggccgg
     1261 gctgcggaaa ccacaccggc gcgctccagc gcataggcga cgcgcagcgc gatatcttcc
     1321 cgccaggggg cggcaatgat ctgcacgccg atcggcatcg gctgcagcgg aaccggcacc
     1381 gcgaccaccg gaagcccgat gaacgaaatc ggctgggtgt ggatgccgat attggcgcgc
     1441 accggaagct cgacgccgtc gagcacgaag gtcgcctgcc cgagttttgg cgcgacgcag
     1501 ggcgtggccg gggcgatgat cacgtcgacg gatttgaaca gctccagcac gcttgcgcgg
     1561 taccagcggc gaaacttttg cgcgcggtcg accagtggcg ccggaatcat cgcgcctgcc
     1621 agcaagcggt cgcgcaccgc cggatcgaaa tcaccggggc gttggcgcag gcgatcgaga
     1681 tgcagcgacg ccccttcggt ggtggagatg acataggccg ccgcgcgcgc gcgggcggct
     1741 tcgggaatct cgatggcgcg cgtcgcgccg agcgccttgg ccacgcgcgc gacggcctca
     1801 acggcctccg gaaacacgtt gtgctgaaaa taaccgccgg cgatcgcgat gcgaacactg
     1861 ccgatgtcct gcgccagcag cggcgtcacc ggctcggccg gccgggtcgt gcatgcggca
     1921 tcctccggat cgggtccctg catcgcgtca taggccagcg cgagatcgtc gacgctgcgc
     1981 gcgaacggcc caagatggtc gaagctcgcg acaaatggaa acgaacgtgc gcgcgacaag
     2041 cggccatagg tcggcttcaa gccaaaaatt ccgcagaacg acgacggcac ccggatcgat
     2101 ccgttggtat ccgagcccag cgccagcggc accaggccgc cgccgaccgc gccgccggag
     2161 ccgcccgacg agccgccggt catccgggtc acatcatgcg gattgcgcga cggaccgtcg
     2221 tgcacgttct cgccagtgaa gtcgtaggcg tattcgccca tattcagcgc cccgaccagc
     2281 accgcgccgg cggcgtccag gcgctcgatc agggtggcgt cgcgcggcga cggcgcaaga
     2341 tcgcggttga tcttcgatcc ggcgcgggtc ggcaggccct gcacatcgaa caggtttttt
     2401 accgcgtacg gcacgccggc gagcggaccg ggattctgcc cggccgtaac ggcggcatcg
     2461 accgcacgcg ctttcgcccg cgcgcgatcg gcggtcacat cggtgaacgc attcaacacg
     2521 ggatcgtgtt tggcaattcg cgccagcgcc gcttcggtga cgccgagggc agagatcttt
     2581 cgactggtta ccgcctgcgc aatccgcaag gccgacagat cgtcgggatt tgcgatcatt
     2641 gcggcgtcag gctgcaaaga cactggccgg ctcggtttcg tcaggcagca cgaattcatc
     2701 caccagccgc gccagcctca gcgacacctc gaggttggcc ttgaccgccg gcttccaggc
     2761 ttcctcgatc ggcagcgcca atgcgttcga gacggcgtcg atataggcat ccagaggatc
     2821 agcagccata cttctcccaa tcgatacttc agtgaaccgg caacggaggg tgcggaatcg
     2881 ccgtcagcag ttcccttgta taggtatcct gcggatcacc caagacccgt tcggacggac
     2941 cctgttcgac gatgcggccc gttcgcatca caatgacgcg atcgcacaat agccgcacca
     3001 cattcaaatc atgcgacacg aacaaatagc tcatacccat cgactgcttc aagtcgtgga
     3061 gcagattcag caccaccgcc tgcaccgaga cgtcgagcgc cgcggtcggc tcgtcgagga
     3121 tcacaagttt gggatgcagc gcgatcgcgc gcgcgatgcc gacgcgggct ttctgtccac
     3181 cggacaactg atgcgggaaa cggtcgagca ggttgaccgg caggccgacc aggccggcca
     3241 gttcctcgca gcgggcccgc aaggcgtcgc gccccttgat atcgccaagc tgcatgatcg
     3301 gatcgacgat cgcgcgcgcg gcggtaaagc gcggattgag gctgtcggtc ggatcctgaa
     3361 acaccatctg gatgctcttg cgcagcggca gccgcgcgaa cgcattgggc aaaatggcgc
     3421 tgatctcgtc gccgtcgaac acgatgcggc ccgaggtctg gtccaacagc cgcatcacca
     3481 tcatcgacgt ggtggatttg ccgcagccgg attcgccgac cagaccgacg ctctcgccat
     3541 ggcccacgct aaagctgatg ccgtcgaccg cgcggaattg ttcggtttcg accggcggct
     3601 tgcgcccaaa caatttgccg agcgtggcgg tcgcgccctg gcgcggatat tccttgacga
     3661 gtttttcgac gaggagaagg ggttttgcgc cactgtcccc acttgctgaa ccgtcacccc
     3721 cgggcttgac ccgggggtcc atcgcttttg acgaagagtc ttgcgaagaa ggatggatgg
     3781 tcgggtcaag cccggccatg acagctgccg cctcctcctc cggcaacaga tctcgcaggg
     3841 aaacgccgag ccgcggcgtc gcgcgcatca gcttcctggt gtaggcgtgt tccggcctgg
     3901 caaagatatc ggccgacagc gcggtttcga ccacccgccc cttctccatc accaccacgc
     3961 ggtcgcaata ggccgcggcc aagccgagat cgtgcgtgat caggatggtc gacatgcccc
     4021 gccgcctggt gagctcgacg atcaggtcca tcaccgcctt ctgcgtggtg acgtcgagac
     4081 cggtggtcgg ctcgtcggcg atcagaagct gcggattgca ggccggcgcc agcgcgatca
     4141 cgacgcgctg gcacatgccg ccggacagtt cgaacggata ggcatgatag cgctcgcgcg
     4201 gacgcgcaat cttgacctgc tcgagcgcct cgatcgcctt ctcgccgcgg tcgctggcgg
     4261 ccgactgcac atgctggcgc agcacgtcct cgatctggcg gccgatcttg cggatcggat
     4321 tgagcgccag ccgggggctc tgaaaaatca tcgagatttc gcggccgcgc aggtcgcgca
     4381 tctggttctc ggtcgctgac ttgacgtcga cgccggagaa catcaccgag ccttcggcga
     4441 tcttgccggc gcggtcgaga atccgcatca ccgcgtagga agtgaccgat ttgccggagc
     4501 cggattcgcc gacgatgccg agcgtctcgc ccttggccac ggagatgttg acatgctgca
     4561 ccgccttgac gatgccgcgc cgggtggcga attccaccgt gagatcctcg acgtcgagca
     4621 gcggctgcgc ggtcatggcg tgttcccgat ttctcgctcg tcatgcgcgg gcttgacccg
     4681 cgcatccatc aagaatccaa agatgctctt cgagcgatgg attgccgggt caagcccggc
     4741 aatgacgaca atgtcggggg ctgctccgcc catgcctcta cgtcctccgc tgcggatcga
     4801 ccacgtcgcg caacccgtcg ccgagcaggt tgaagcagaa taccgcgatc atcaacgcgc
     4861 cgccggggaa gaaggcgacc caccactcac cggaaatgat gttggtcgca ccttccgcga
     4921 ccatgatgcc ccattcgggg gtcggcggct tgaccccgag gccgatgaac gacagaccgg
     4981 ccgcgttcag aatggcgtaa cccatggtca gcgatatctg caccatcatg atcggcatga
     5041 tgttcggcag gatatgcacc atcacaatgc gaaaatcact gttgccggac agccgcgccg
     5101 cctgcacaaa gccggcgtcg cgccggacat tggcttcggc gcgcgcgacc cgcacataga
     5161 gcggaaaatt gatgatcgcg gtcgcgatga cgatgttggt caccgtattg ccgagggccg
     5221 ccacgatacc catcgccagc acgaacaacg gaaacgccat gatggtgtcg gagatgcgtc
     5281 cgacgatgcg atcggtccac ccgccgaaga agccggcggc gatcccggcg aggccgccca
     5341 tcaccagcac cagcgccacc gaagcgacgg cgatcacaaa atcgaggcgt gtcgccacca
     5401 gcacacggct gaagatgtcg cggcccagca aatcggtacc gaaccaatgg tgcaacgacg
     5461 gcgactgcag ggtcgccgag gtgtcgctgg cgagcggatc gtatggcgca atgtaagggc
     5521 cgatcaaggc gcaaagcgcg atgatgacga acagcccgaa cgacaagccg gtcaccggat
     5581 tttccgccac cacataacgg gcatggcgga aaatggagtt ggccggaggc ttgggttgaa
     5641 cctcggccgc cggagcggcg gtcagactgg cttcgctcat gcctcgagcc tcacgcgcgg
     5701 gtcgatcacg ctgtagagca tgtcgatcaa gaggttgagt gcgacgtaca ggaccgccat
     5761 ggtcagcacg aagccctgca ccggcgcgaa gtccgactgc agcaacgcct cgacggcgta
     5821 ggagccgatg cccggccatg cgaacacttt ttccaccagc acattggcgc cgagcaggaa
     5881 cgagaacacc atacccaacg tggtcacgac cggcagcatc gcgttgcgga agccgtaggt
     5941 caggatgacg gtgcggtcat cgagaccgct ggcgcgcgcg gttcgtatga actcggacga
     6001 cagcaccgcc agcatcgagg cgcgggtcat gcgcgtgatc ggcgccagcg agaaaatcgc
     6061 caatgtcgcc gccggcagga tcaactggct gagcgaggcg cggaaggtct cgaaatttcc
     6121 ggtcaccagg ctgtcgatca gatagaagcc ggtgatatcg ggcggcgcgg tggcgaaggc
     6181 atcgagccgg ccgatcggcg ccggcgacca gccgagcttg aaatagaaga tatagaccag
     6241 cagcaggccg gtgaagaaaa ccggcagcga aacgccggcg gtggcgacaa ttctgcacaa
     6301 atgatcgatc agggagcctt gcttgactgc ggcgaaaatg ccgagcggca ccgcgatgac
     6361 gatggcgagg aacagtccca gcagcgtgag ctcagcggat gccggcagcc ggctggtgat
     6421 ttcggttgcc accggacggc cggtggaaag cgagttgccg aaattgccgt gggccagatc
     6481 attgacataa tgcgcgaact ggtcgggcag cggccggtcg agcccg
//
LOCUS       DATNOI010000440         7081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1445849,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000440 DATNOI010000000
VERSION     DATNOI010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445849"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /gene="atpD"
                     /locus_tag="VN968_17595"
     CDS             <1..994
                     /gene="atpD"
                     /locus_tag="VN968_17595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HXL31082.1"
                     /translation="GGKIGLFGGAGVGKTVLIQELINNVAKAHGGYSVFAGVGERTRE
                     GNDLYHEFIESGVNKKGGGEGSKCALVYGQMNEPPGARARVGLTGLTVAEHFRDQGQD
                     VLFFVDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGAMQERITTTTKGSITSIQ
                     AIYVPADDYTDPAPATTFAHLDATTNLDRAISEKGIYPAVNPLDSTSRMLSPLIVGEE
                     HYQTARMVQQILQKYKSLQDIIAILGMDELNEEDKITVARARKIERFLSQPFFVAEVF
                     TGSPGKFVDLADTIKGFRGLCEGKYDHLPEQAFYMVGTIEEAVEKGKKLAAEAA"
     gene            1097..1504
                     /locus_tag="VN968_17600"
     CDS             1097..1504
                     /locus_tag="VN968_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HXL31083.1"
                     /translation="MATFHFDLVSPEKLAFSGDVDQVDVPGIEGDFGVLAGHAPVVAA
                     IRPGILTVMSGGTQQKIIVLGGLAEVSDKGLTVLADVATSLQDLDRAQFADKISEMET
                     KLAEKDGSELDRAIERLDHYKSIQHQLNATAMH"
     gene            1746..3368
                     /locus_tag="VN968_17605"
     CDS             1746..3368
                     /locus_tag="VN968_17605"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL31084.1"
                     /translation="MKRKIAAIFAADIAGYSRLVAEDEEETLRRLASYRQVTDDFIAR
                     SGGRIFNTAGDAVLAEFPSAVEAVRCAIDIQESLRTRNMAYPASRHMSFRIGITIGDV
                     VERDGDLLGDGVNIAARLEGLAEVGGICISRAVHEQVANKLSVQFADIGAQEVKNIPT
                     PVHAFMLAMRREDGSYSMPKMKKKPAAGGTTPSWMWPAAVVFIVSIGVGGFLYFVKVK
                     MPDGYRAAAPASNEAAAISSPPVAPIASPVPTTAAPAPPPSASPPGPLSEKFAAETLP
                     FVSDRTRLALSNEYALASDHKAFALNVTGTYAIATSQQNEELAKTAALEQCQKKADAI
                     QSPRKCELYAVGNTVVYPHGKPPLPPLPWIRHDPSTEKPYATRDMPLVRDIGKSRLES
                     YYVPGKKTKSIAVSPGGLFIPVVGAETIEDSARRSLESCGAIAGVPCMLVAVDDVFVV
                     PVPTMLKVTGFFQAAGSSSIAADARDDVSRKLMDASSGWNAVAVGAARRPGLALKAAS
                     EPNAVNEALGNCLKHDSDCRVIAIGPFSVGPN"
     gene            complement(3375..3875)
                     /locus_tag="VN968_17610"
     CDS             complement(3375..3875)
                     /locus_tag="VN968_17610"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HXL31085.1"
                     /translation="MTETKPYRPNVGIALFNAAGQVLIGRRFKDDGPEIILPGLEWQM
                     PQGGIDTNENPRDAVMRELWEETGVTNASYLGEADWLTYEFPPFAVPPSHRLAKFRGQ
                     RQKWFALRFTGSDDEIDPLTPRNGQPAEFDQWRWERLDRVADLVVPFRREVYRTVAQA
                     FAKFAR"
     gene            complement(4023..4526)
                     /locus_tag="VN968_17615"
     CDS             complement(4023..4526)
                     /locus_tag="VN968_17615"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HXL31086.1"
                     /translation="MARYEDLPYRTCVGMMLINEAGLVFIGRRAGGIEHVDQSHVWQM
                     PQGGVDPGEDTWAAAKRELYEETSVRSVEKLGEVADWLIYDIPRTVAGRTWKGRYRGQ
                     RQKWFAVRFTGHDAEINVAAPGGGHKAEFVSWRWEPMKNLPDLIVPFKRPVYERVVKE
                     FSKLAGS"
     gene            complement(4600..5805)
                     /locus_tag="VN968_17620"
     CDS             complement(4600..5805)
                     /locus_tag="VN968_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HXL31087.1"
                     /translation="MAETADDLSTPLGQKTLRAKHRFRLPFTFTQALAVLLGLFLVVF
                     AGFAIFNDNPLGGEPIAHIALREATPPGEKPAAAHPSASVPVIKSAIQQAAPGEHKTV
                     TIIDGSSGARHDVTISGDGPDKVEAASASATMAGIDQRLLEKSRYGMIPVVAADGLKP
                     FTAYAAEADRARAAKMPVVAIVVGGLGVGAAKTTEAIMKLPAAVTLAFTPYGADPTKQ
                     TERARAQRHEILLQIPMEPFDYPDNDPGPQTLLTTLGSEQNLDRLYWHLSRFQGYAGI
                     ANFMGGRFVVTDAAMQPIIREAAKRGLGFLDDGSAPHSVASTLASGQSVPFARADLSI
                     DAVPTTAEIDRALAKLESLAKERGTAVGIAAALPVSIERIGVWIKALEGHGIMLVPLT
                     SAMLKSKSG"
     gene            complement(5999..>7081)
                     /locus_tag="VN968_17625"
     CDS             complement(5999..>7081)
                     /locus_tag="VN968_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXL31088.1"
                     /translation="FRDMQVQTRGEFGGLGIEVTMEDGLIKVVSPIDDTPASKAGIMA
                     NDIITNLDDEAVQGLTLNQAVEKMRGPVNTKIRLKIIRKGQDNPLDVTLVRDNIRVRS
                     VRARVEGDDIGYIRITTFNEQTTEGLKREIGNLSTQLGDKLKGYIIDLRNNPGGLLEE
                     AVTVSDTFLERGEIVSTRGRNAEETQRRAAHPGDLAKGKPLIVLINGGSASASEIVAG
                     ALQDHKRATLVGTRSFGKGSVQTIIPLGSGNGALRLTTARYFTPSGKSIQAKGIVPDI
                     EVLQDVPDELKSRTDTKGEASLRGHLKSEGDEKTGSQSYVPPDAKDDKALKMAADLLH
                     GVKVNAAAPATGDKAAIDKPTNKEAN"
BASE COUNT         1280 a         2210 c         2235 g         1356 t
ORIGIN      
        1 gggcggcaag atcggcctgt tcggcggcgc cggtgtcggc aagaccgtgc tgattcagga
       61 actgatcaac aacgtcgcca aggcgcatgg cggttattcg gtgttcgccg gcgtcggcga
      121 gcggacccgc gaaggcaacg atctctatca cgagttcatc gaatccggcg tcaacaagaa
      181 gggtggcggc gaaggctcca aatgcgcgct ggtctacggc cagatgaacg agccgccggg
      241 cgcccgcgcc cgcgtcggcc tcaccggtct caccgtcgcc gagcatttcc gcgatcaggg
      301 ccaggacgtg ctgttctttg tcgacaacat cttccgcttt acgcaagcgg gttccgaagt
      361 gtcggcgctg ctgggacgta ttcccagcgc cgtgggctat cagccgacgc tcgccaccga
      421 catgggcgcg atgcaggagc gcatcaccac caccaccaag ggctcgatca cctcgatcca
      481 ggcaatttac gtccccgcag acgactacac cgacccggcg ccagcgacga cgtttgccca
      541 tctcgatgca acgaccaacc tcgatcgcgc aatctcggag aaggggatct atccggcggt
      601 caacccgctg gattcaacat cgcggatgct gtcgccgctg atcgttggcg aggagcatta
      661 ccagaccgcg cgcatggttc agcagatact gcagaagtac aagtcgctgc aggacatcat
      721 cgccattctc ggcatggacg aattgaacga agaggacaag atcactgtcg cgcgcgcccg
      781 caagatcgag cgcttcctgt cgcagccgtt cttcgtcgcc gaagttttta cgggatcgcc
      841 gggcaaattc gtcgacctcg ccgacaccat caagggcttc cgcggacttt gcgaaggcaa
      901 gtacgaccac ctgcccgagc aggccttcta catggtcggc accatcgaag aggccgtcga
      961 aaagggcaag aagctggctg ccgaggcagc ctaaggagcg aatggcgaat agggagtagc
     1021 gaatagagaa gatattcgcc tttccattcg ctactcgcca ttcgctactc gccacttact
     1081 actcgcaggt actcccatgg ccaccttcca ctttgacctc gtctcgccgg aaaaactcgc
     1141 cttctcgggc gacgtcgatc aggtcgatgt ccccggcatc gagggcgatt tcggcgtgct
     1201 cgccggacat gcgccggtgg tggccgcgat ccggccggga atcctgacgg tgatgtcggg
     1261 cggcacccag cagaagatca tcgtgctcgg cggtctggcc gaagtgtccg acaagggttt
     1321 gacggtgctg gcggacgtcg ccacttcgtt gcaagacctc gaccgggccc agttcgccga
     1381 caagatttcc gagatggaga ccaagcttgc cgagaaggac ggctccgaac tcgaccgcgc
     1441 gatcgagcgg ctcgatcatt acaagagcat ccagcaccag ctcaacgcga cggcgatgca
     1501 ctagagcctt tccgtttcga tagaatcgaa acggggctct agcttcttga tttgacgcgt
     1561 tttcttcacg cgaaccggtc gccgcttcgc ttgaaaacgc ttcggcgcgg cgtgccgcgc
     1621 gagactttct tcaaaactga acgccacagc gccgtggcga cgtcacgagc gctgaaaccc
     1681 ggttgcgccc tgcgccttcc agcggcattc tgctgcaata gatggttgtc cgggggccga
     1741 cctttatgaa gcgcaagatc gcggcgattt ttgcggccga tattgctggc tacagcaggc
     1801 tggttgccga agatgaagag gaaacgctgc ggcgcctcgc atcctatcgt caggtgaccg
     1861 acgacttcat cgccagatcc ggcgggcgta ttttcaacac cgccggtgac gccgttctcg
     1921 ccgaatttcc aagcgcggtc gaagcggtgc gttgcgcgat cgacatccag gagagcctgc
     1981 gtactcgcaa catggcgtat cccgccagcc gtcacatgag ctttcggatc ggcatcacga
     2041 tcggcgacgt ggtggagcgc gacggggacc tgctcggcga tggcgtcaat atcgcagccc
     2101 gcctggaagg tctcgccgag gtcggcggca tctgcatttc gcgcgcggtg catgagcagg
     2161 tcgccaacaa gctgtcggtg cagttcgccg atatcggcgc gcaggaagtg aagaatattc
     2221 ccactcccgt gcatgccttc atgctggcga tgcggcgtga ggacggcagc tattccatgc
     2281 cgaaaatgaa gaagaagccc gccgcgggag ggacaacgcc aagttggatg tggccggcgg
     2341 cggtggtgtt tatcgtctcc atcggtgtcg gcggcttcct gtattttgtc aaagtgaaaa
     2401 tgccggacgg gtacagggcg gcagctccgg cgagcaatga agcggctgca atatcttcgc
     2461 cgccggtcgc tccgattgcg tctcccgtcc caacgacagc cgcaccggcg cctccgccat
     2521 cggcgtcgcc gcctggtccc ttgagcgaaa agttcgcggc cgaaaccctg ccgttcgtca
     2581 gcgatcgcac ccgtctcgcg ctttcgaacg agtacgcact ggcgtcagac cacaaggctt
     2641 tcgccctcaa tgtgacgggc acctacgcga tcgccaccag tcagcagaac gaagaactcg
     2701 cgaaaactgc ggcgctcgaa caatgccaga aaaaagccga cgccattcag tcgccgcgaa
     2761 aatgcgaact ttacgccgtc ggcaacaccg tggtctatcc gcatggcaag ccgccgctgc
     2821 cgccgttgcc ctggatcagg catgatcctt caaccgagaa accctatgcg accagggaca
     2881 tgccgctggt acgggacatc ggaaagagca gacttgagag ctattatgtg ccgggcaaaa
     2941 agaccaagtc gatcgcggtc agtccagggg ggctattcat tcccgttgtc ggcgccgaaa
     3001 cgatcgagga ttcggcgcgt cgaagcctgg aatcctgcgg cgccattgcc ggcgttccct
     3061 gcatgctcgt ggccgtcgac gatgtctttg ttgtgccggt gccgaccatg ctgaaggtca
     3121 ccggtttctt tcaggccgcc ggcagttctt ccatagccgc cgatgcgagg gatgacgtgt
     3181 cccgtaaact catggatgca tccagcggat ggaacgcggt cgccgtcggc gcggccaggc
     3241 gtccgggact ggcgctcaag gcggccagcg aaccaaatgc ggtcaacgaa gccttgggca
     3301 actgcctcaa gcacgacagc gattgccgcg tcatcgcgat cggtccgttc tcggtcgggc
     3361 cgaactgagc tttatcatcg cgcgaatttc gcaaatgcct gcgccaccgt ccgatacacc
     3421 tcgcgccgga acggcaccac gaggtcggcg acgcggtcga gccgttccca tcgccattgg
     3481 tcgaattcgg ccggctgacc gttacgtggt gtcagcggat cgatttcgtc atcggacccc
     3541 gtgaaacgca gcgcgaacca tttctggcgc tggccgcgga atttggcgag ccgatgcgac
     3601 ggcggcacgg cgaatggcgg aaactcgtac gtcagccagt cggcttcgcc gagatagctg
     3661 gcgttggtca cgccggtctc ttcccataat tcgcgcatca ccgcgtcgcg cggattttca
     3721 ttggtgtcga taccgccttg cggcatctgc cattcgaggc cgggaagaat gatctccggt
     3781 ccgtcgtcct tgaagcgccg cccgatcagc acttggccgg cagcgttgaa caacgcgatc
     3841 cctacattgg gacgataggg tttggtctcg gtcatcaact caacggctgt cattccgaga
     3901 tgcgccgctt tggcgcagac ctcagatgtg cactgcacat cgggcaatct ggagattccg
     3961 ggttcatgct tcgcatgccc cggaatgacg tcactgcagg gaaaggcttc cgaaccgtca
     4021 tcctagctgc ccgccagctt cgaaaattcc ttcaccacgc gctcatagac cgggcgcttg
     4081 aacggaacaa tcaggtcggg aagatttttc atcggctccc agcgccagct gacgaattca
     4141 gccttgtggc cgccacccgg ggcggcgacg ttgatctcgg cgtcgtggcc ggtgaagcga
     4201 accgcgaacc acttttgccg ctggcctcga tagcggccct tccaggtccg tccggcgacg
     4261 gtgcggggaa tgtcatagat cagccagtcc gcgacctcgc cgagcttctc gaccgatcgg
     4321 acgctggtct cctcataaag ttcacgcttc gccgccgccc aggtatcctc gccgggatcg
     4381 actccgcctt gcggcatctg ccagacatgg gattggtcga catgttcgat gccgccggcg
     4441 cggcgaccga tgaagaccag cccagcttcg ttgatcagca tcatgccgac gcatgttcga
     4501 tagggcaggt cttcgtagcg cgccattccg tgaaagcctc taacggtcat atgggcgaga
     4561 cccggccggt tcgaaaggcg cgccagcccg aaacttgcct cagcccgatt ttgatttcag
     4621 catcgcgctt gtcaatggca caagcatgat gccatggccc tcaagcgcct tgatccagac
     4681 gccgatccgc tcgatcgaga ccggcagcgc cgcggcgatg ccgacggcgg ttccgcgttc
     4741 cttcgccaga ctttcaagct ttgccagggc ccggtcgatt tccgctgtcg tcggcaccgc
     4801 atcgatgctg agatcggccc tggcgaaagg caccgattgg ccgctcgcca gcgtggaagc
     4861 cacgctgtgc ggtgcggaac cgtcgtcaag gaagccgagc ccgcgtttgg cggcctcgcg
     4921 gatgatgggc tgcatggcgg cgtcggtaac aacgaaacgc ccgcccatga aattggcgat
     4981 cccggcatag ccctggaacc ggctgaggtg ccagtacagc cggtcgaggt tctgctcact
     5041 gcccagcgtc gtcagcaggg tttgcgggcc ggggtcgttg tcgggatagt cgaacggttc
     5101 catcggtatc tgcaatagaa tttcgtggcg ctgcgcgcgc gcccgttcgg tctgcttggt
     5161 gggatccgcg ccgtagggcg taaacgccag cgtcaccgcg gcgggcaact tcatgatggc
     5221 ctcggtggtc ttggcggcgc cgaccccgag gccgccgacg acgatggcga cgacaggcat
     5281 tttcgcggcc ctggcgcggt cggcctcggc ggcgtaggcc gtgaacggct tcagcccgtc
     5341 ggcagcaact accggaatca tgccgtagcg tgatttctcg agcagccgct gatctatccc
     5401 ggccatcgtc gcggatgcgg acgcggcctc gaccttgtcc gggccgtcgc cggatattgt
     5461 gacatcgtga cgcgcgccgc tggatccgtc gatgatggtg acggtcttgt gctcgcccgg
     5521 cgcggcctgc tggatcgcgg attttatgac cggcacggaa gcgctggggt gcgccgcggc
     5581 gggtttctcg cccggcgggg tcgcttcacg cagcgcgata tgcgcgatcg gttcgccgcc
     5641 gagtgggttg tcgttgaaga tagcgaagcc cgcgaagacg accagaaata ggcccagcag
     5701 cacggcaagc gcctgggtga aggtaaaggg caaccggaac cggtgcttcg cgcgcagcgt
     5761 cttctgtccg agcggtgtgc tcagatcgtc ggccgtctct gccatgaatc tccccgaatc
     5821 agccggggcg gacgatacca cggcggggca agaccatgac gcctgcgggg caagacgatg
     5881 gctgtcccca tgcaaaaggc cgtctcaggt tcggcggcgg gcgatccaag aaaccatcag
     5941 caaaaagggc ggcccgaagg ccgccctttt cgtaaaacag ctggctggtt gtgccggatc
     6001 agttcgcctc tttgttggtg ggcttgtcga tcgccgcctt gtcgccggtg gcgggcgcgg
     6061 cggcattgac cttgacgccg tgcagcaggt cggctgccat cttcaaggcc ttgtcgtcct
     6121 tggcatccgg cgggacgtag gattgcgaac cggtcttctc gtcgccctcg gacttcagat
     6181 ggccgcgcag cgaggcttcg cccttggtgt cggtccgcga cttcagctcg tcgggcacgt
     6241 cctgcagcac ctcgatgtca ggaacgatgc ccttggcctg gatcgatttg cccgatggcg
     6301 tgaagtagcg cgccgtggtg agccgcagcg cgccgttgcc ggaaccgagc ggaatgatgg
     6361 tttgcaccga gcccttgccg aacgagcggg tgccgaccag cgtggcgcgc ttgtgatcct
     6421 gcagcgcgcc ggcgacgatc tcggaagccg atgccgagcc gccattgatc aggacgatca
     6481 gcggcttgcc cttggcgagg tcgcccggat gggcggcgcg gcgttgggtt tcctcggcgt
     6541 tgcggccacg ggtcgaaacg atctcgccgc gctcgaggaa ggtatcggac accgtcacgg
     6601 cttcttccag caagccgccg ggattgttgc gcaggtcgat gatgtaaccc ttcaacttgt
     6661 cgccgagctg tgtgctcagg ttgccgatct cccgcttcag gccttcggtg gtctgttcgt
     6721 tgaacgtcgt gatgcggatg tagccgatat cgtcgccttc gacgcgcgcg cgcaccgagc
     6781 ggacgcggat gttgtcgcgc accagcgtga cgtcgagcgg attgtcctga cccttgcgaa
     6841 tgatcttgag ccggatcttg gtgttgacgg gcccgcgcat tttttcgaca gcctgattga
     6901 gggtgaggcc ctggaccgct tcgtcgtcga gattggtgat gatgtcgttg gccatgatgc
     6961 cggccttgga ggcgggcgta tcatcgatcg gcgagacgac cttgatcagc ccgtcttcca
     7021 tcgtgacttc gatgccgagc ccgccgaatt ccccgcgggt ctgcacctgc atgtcgcgga
     7081 a
//
LOCUS       DATNOI010000441         4889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2286710,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000441 DATNOI010000000
VERSION     DATNOI010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2286710"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /gene="ruvA"
                     /locus_tag="VN968_17630"
     CDS             complement(<1..335)
                     /gene="ruvA"
                     /locus_tag="VN968_17630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HXL31089.1"
                     /translation="MIGKLKGLIDSYGEDFVILDVNGVGYQVHCSSRTLQALPSPGEA
                     AVLSIETYVREDQIKLFGFRTDAEREWFRLLQTVQGVGAKVALAVLGTLPPAELANAI
                     ALRDKAAVSR"
     gene            complement(553..1080)
                     /gene="ruvC"
                     /locus_tag="VN968_17635"
     CDS             complement(553..1080)
                     /gene="ruvC"
                     /locus_tag="VN968_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HXL31090.1"
                     /translation="MTHQPIRPAVRILGIDPGLRRTGWGVIEIDGNRLSFVGCGSVEP
                     PEGLPLASRLLAIHEGLAAVLGDFRPAEAAVEQTFVNKDGVATLKLGQARGVAMLAPA
                     MFGISVAEYAPNQVKKTVVGAGHADKNQILVMLKILLPKAEPKSADAADALAIAITHA
                     HHRQSAALRLRVVGL"
     gene            complement(1297..2043)
                     /locus_tag="VN968_17640"
     CDS             complement(1297..2043)
                     /locus_tag="VN968_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXL31091.1"
                     /translation="MAGHSQFKNIMHRKGRQDAQKSKLFSKLAREITVAAKMGQPDPS
                     MNARLRAAITSARQENMSKDSIERAVKKAIGGDAENYDEIRYEGYGPGGVAVIVEALT
                     DNRNRAASDIRSYFTKSGGNLGETGSVAFMFDHTGIIEYDAKVASDDAMLDAAIEAGA
                     DDVLSSENGHEIYASQDTFREVAKALEAKFGEARKAALTWKPQNTVPVDDETGEKLLK
                     LIDLLNEHDDVQNVYANFEVSDALMTKMGG"
     gene            2244..4004
                     /locus_tag="VN968_17645"
     CDS             2244..4004
                     /locus_tag="VN968_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL31092.1"
                     /translation="MAFGLFRKRLPDPAAPTAIPVAATPTIAADVAGVSDSDSAREIL
                     ELLELELGAMIRQLERAAHSVADGAEATAATLSTIRQRANALTGHTSAAQGTATTFSQ
                     AADNFTHSAQGIGSQVRDAGKLADEASAAAEEASLNVGRLRESSAAIGNVVNLIAQIA
                     RQTTLLALNSTIEAARAGDAGRGFAVVATEVKALAVQTQHATEEITKRIDALQRDAAG
                     SVDAVHRISQAIAAIRPVFENVNGAVAEQNETTGKMSDNAASASQFIVSVGDSAAKID
                     SATREAEAHGKNVAEAGSAVTTFTEKLKSRCAVLLRQGEREDRRKRARLPCHLKIEIQ
                     TARGPVAAPVYEISMEGILIGGPDAERLPLNENLNAALENVGACRIRLGEHSKAGALA
                     RFERPDAALSEKIEDALWALHDENIEFVTRAMEAGTALTRIFEDAVASSAISVDDMFD
                     ADYVEISGTNPLQHRTRILDWADRALPPFQEAFLVRDPQMAFCVMIDRNGYLPVHNRI
                     YSHPQRSGDVVWNTANSRNRRIFNDPAGLAAGRNLRSYLIQSYARDMGNGKTVMMREI
                     DVPVRVNGRHWGGFRTAYKL"
     gene            4069..>4889
                     /locus_tag="VN968_17650"
     CDS             4069..>4889
                     /locus_tag="VN968_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL31093.1"
                     /translation="MPVAEPAVLEVAAAPPSASERLVDQLASRIGGLGVELADVAGNL
                     QEVAARVSGQSERFGHLQKTAETMVSANHNIATASRAVQSATSAAVGEITQSRAVVET
                     AVKHIAELIDAVRRIELSLGSVGTALAQVAKVSGSIEAIAKQTNLLALNATIEAARAG
                     AAGRGFAVVASEVKNLAEATRQATHLIGETVRDLDGQVGHLISASGDASLRAKNAGEG
                     AQQIQSIIVRVQDGFTSVGQEIDGVAKAATSNLAHCDLVISELGNLAKGVDLSST"
BASE COUNT          905 a         1585 c         1545 g          854 t
ORIGIN      
        1 cgcgacaccg ccgccttgtc gcgtagcgca atcgcgttgg cgagttcggc cggcggcaag
       61 gtgccgagca ccgcgagcgc caccttggcg ccgacgccct gcacggtctg caacaggcgg
      121 aaccattcgc gctcggcatc ggtgcggaag ccgaacagtt ttatttgatc ctcgcggaca
      181 taggtctcga tcgagagcac cgcggcttcg ccgggcgacg gcagcgcctg cagcgtgcgc
      241 gacgagcaat gcacctggta gccgacgccg ttgacgtcga gaatgacaaa atcctcgccg
      301 taagagtcga tcaagccttt gagtttaccg atcatggtga ctgctcggac aaggcacgca
      361 catttcgttc gtcatgcccg ggcatagcag tctggcttgc gcagactgcg taaacttgtc
      421 tgcgatcccg ggcatccacg tctttttttg ccgacgtccc agacgtggat ggccgggcat
      481 aggcgagcgg aagcgacgcc gtccttcgga cgactatgcc cggccatgac ggctgagatt
      541 gatgccaatc cctcatagcc ccaccaccct cagccgcagc gcggcgctct ggcggtgatg
      601 cgcgtgggtg atggcgatgg cgagcgcgtc ggcggcatcg gcggatttgg gctcggcctt
      661 gggcagcagg attttcagca tcaccagaat ctggttcttg tcggcatgtc cggcgccgac
      721 cacggtcttc ttcacctgat tgggcgcgta ttccgcgacc gaaatcccga acatcgccgg
      781 cgccagcatc gcgacgccgc gcgcctggcc cagcttcagc gtcgcaaccc cgtccttgtt
      841 gacaaaagtt tgttcgaccg cggcttcggc gggacggaag tcgccgagca cggcggcgag
      901 accctcgtga atcgccaaca ggcggctggc cagcggcaag ccttccggcg gttccaccga
      961 gccgcagccg acgaaactca gccggttgcc gtcgatctcg atcacccccc agccggtgcg
     1021 gcgcagcccc ggatcgatgc caaggatgcg gacggcaggg cgaatcggct gatgggtcat
     1081 gcctaagtgt taacgccgga gcatggcgaa cgaaatagaa acggggcgtc ataccccacg
     1141 aaagcggtcg acttacaccc gtcatgcgcg ggcttgaccc gcgcatccat ccaacatgac
     1201 aggtcgattc aagcgatgga ttgccgggca taggcgagcg gaagcgacgc cgtccttcgg
     1261 acggctatgc ccggcaatga cgaatggatg cttgccctac ccgcccatct tggtcatcag
     1321 cgcgtcggaa acctcgaaat tcgcatacac gttttgcacg tcgtcgtgct cattcaagag
     1381 atcgatcagc ttcaacagct tctcgccggt ctcgtcgtcg accggcaccg tattctgcgg
     1441 tttccaggtt agcgccgcct tgcgggcttc gccgaatttg gcctccagcg ccttggcgac
     1501 ctcgcgaaag gtgtcctgcg aggcgtagat ctcgtggccg ttctcgctcg acaatacgtc
     1561 gtcggcgccg gcctcgatcg ccgcatccag catcgcatcg tcggaggcga ccttggcgtc
     1621 gtattcgatg atgccggtgt ggtcgaacat gaaggcgacc gaaccggttt cgccgagatt
     1681 gccgccggat ttggtgaagt aggaccggat atcggacgcc gcgcggttgc ggttgtcggt
     1741 cagcgcctcg acgataacgg cgacgccgcc cgggccataa ccctcgtagc gaatctcgtc
     1801 gtagttttcg gcgtcgccgc caatcgcctt cttcaccgca cgctcgatcg agtcttttga
     1861 catgttctcc tggcgcgcgg aggtgatcgc ggcgcgcagc cgcgcattca tgctggggtc
     1921 cggctggccc atcttggccg cgacggtgat ttcccgcgcc agcttgctga acagtttcga
     1981 cttctgggca tcctgccggc ccttgcggtg catgatgttc ttgaactggg aatgtccggc
     2041 catgcggatg cctctgggat tcgtgcggaa tggtggggtg gaaggcgcgg cgttataggc
     2101 cggtgaccgc ccgaaatcaa agatttaccg ggcctttcgg tatacgggta gcgtccgttg
     2161 cctacccatc gggcaattat taaccatggt ttcacgtccg actgcgagca tgcggtttcc
     2221 tttgcctgtt ttgagattag ccaatggcgt tcggattgtt tcgaaagcgt ctgccggatc
     2281 cggccgcgcc gaccgccata cctgtggcgg cgacgccgac gatcgcggct gacgtagccg
     2341 gcgtttcgga tagcgattcc gccagggaaa tcctcgaact gctcgagctc gagctcggcg
     2401 ccatgatccg gcagctcgaa cgcgcggccc attcggtcgc cgatggcgcc gaggcgacgg
     2461 cggcgacgct ctccaccatt cgacagcgcg ccaacgcgct gaccggacat accagcgccg
     2521 cgcagggcac cgcgaccacc ttttcgcagg ccgcggacaa cttcacccat tccgcgcaag
     2581 gcatcgggtc ccaggtgcgc gacgccggca agctcgccga cgaggccagc gccgccgccg
     2641 aggaggccag cctcaatgtc ggtcgcttgc gcgaatcgtc ggctgccatc ggcaatgtcg
     2701 tcaatctgat cgcgcagatc gccaggcaaa ccacgcttct ggcgctcaac tccaccatcg
     2761 aggcggcgcg ggcgggtgat gccgggcgcg gctttgccgt ggtcgccacc gaggtcaagg
     2821 cgctggcggt gcagacccag cacgccaccg aggagattac caaacggatc gacgcgctgc
     2881 agcgcgacgc ggcgggctcg gtcgacgccg tgcaccggat ttcgcaggcg atcgcggcga
     2941 tccgcccggt tttcgagaac gtcaacggcg cggtggcgga gcagaacgaa accaccggca
     3001 agatgtccga caacgccgcc tccgcatcgc agttcatcgt ctcggtcggc gacagcgccg
     3061 ccaaaatcga cagtgcgacc agggaggccg aggctcatgg caagaacgtc gccgaggccg
     3121 gcagcgccgt caccacgttt acagagaaac tcaaatcacg ctgcgcggta ctgctgcggc
     3181 agggcgagcg cgaagaccgg cgcaagcgtg cgcggctgcc gtgccatctc aagatcgaaa
     3241 ttcagaccgc gcgcggccct gtcgcagcac cggtctacga gatttccatg gaaggcatcc
     3301 tgatcggcgg gccggacgcc gagcggctgc cgctgaacga gaacctcaat gccgcgctgg
     3361 agaacgtcgg cgcctgccgg atacgcctcg gcgagcattc aaaagcaggc gcactggcgc
     3421 ggttcgagcg gccggacgcc gcattgagcg aaaaaatcga agatgcattg tgggcgctcc
     3481 acgacgagaa catcgaattc gtcacccgcg ccatggaagc cggcactgcg ctgaccagga
     3541 tattcgagga cgccgtcgcc agcagcgcga tctccgtcga cgacatgttc gatgccgatt
     3601 atgtcgagat atcaggcacc aaccccttgc agcaccgcac ccggattctc gattgggcgg
     3661 atcgcgcgct gcctccgttc caggaggcct tcctggtcag ggatccgcag atggcgttct
     3721 gcgtgatgat cgaccgcaac ggttacctgc cggtgcacaa caggatctac tcgcatccgc
     3781 aacgatcagg cgatgtcgtg tggaacaccg ccaacagccg caaccgccgc atcttcaacg
     3841 atcccgcggg gctcgccgcc ggccgcaacc tgcgttccta tctgatccag agctacgccc
     3901 gtgacatggg caacggcaaa accgtcatga tgcgcgaaat cgacgtgccg gtccgcgtca
     3961 atggccggca ctggggcggc tttcgcaccg cgtacaagct ttgagtcgta caggcatcca
     4021 aaaatcatga accagtcatc cagtctattg acccggagga acgcagcgat gccggttgcg
     4081 gagcccgcag tattggaagt cgccgcggcg ccgccctccg ccagcgaacg tctggtcgat
     4141 cagctagcca gccggatcgg cggcctcggg gttgaactcg cggacgtggc gggaaatctg
     4201 caagaagtcg ccgctcgggt atccggccaa tccgagcggt tcggacatct tcaaaagacc
     4261 gccgagacga tggtttccgc caaccacaat atcgcaaccg cgtcgcgggc ggtgcagtcg
     4321 gcgacatcgg ccgccgtcgg cgagatcacc caatcgcgcg ccgtggtgga aacggcggtg
     4381 aagcatatcg ccgaattgat cgacgccgtc cgccgtatcg agctcagtct cggttcggtg
     4441 ggaaccgcgc tcgcgcaggt cgccaaggta tccgggtcga tcgaggccat cgccaagcaa
     4501 accaacctgc ttgcgctgaa tgcgaccatc gaggccgccc gcgccggcgc cgcgggaaga
     4561 ggctttgcgg tcgtcgccag cgaggtgaag aacctcgccg aagcgacgcg gcaggcgacg
     4621 catctgatcg gcgaaaccgt tcgcgatctc gacggtcagg tcggccatct gatcagcgcg
     4681 agcggcgacg cctcgcttcg ggccaaaaac gccggcgaag gcgcccagca gattcagagc
     4741 atcatcgtcc gcgtccagga cggttttacg tcggtcggcc aggagatcga cggggtcgcc
     4801 aaagcggcga cttccaatct cgcccattgc gacctggtga tctctgaact tggcaatctc
     4861 gccaagggcg tcgatctgtc gtcgaccga
//
LOCUS       DATNOI010000442         6860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_231323,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000442 DATNOI010000000
VERSION     DATNOI010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231323"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VN968_17655"
     CDS             complement(<1..427)
                     /locus_tag="VN968_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXL31094.1"
                     /translation="MTKPIDTKNSRPAPSAPRRPHAFTTHGITVVDDYAWLKDANWQE
                     VLRDPSGLDADIRNYLEAENDYTESLLGHTAGLQKTLVKEMRGRIKEDDSSVPAPDGA
                     YAYLRKFRDGGEHEMFGRTQRDGGEIEIVLDGDELAADHE"
     gene            complement(519..2237)
                     /locus_tag="VN968_17660"
     CDS             complement(519..2237)
                     /locus_tag="VN968_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXL31095.1"
                     /translation="MTITAGLDATKGKIDWTKPVLALFAVCLILLIVLPLSWLGIYSV
                     IDKANHLTLQNFVTLFTDPDFLDPLLTTAIIATTSATICCLVAAPMGWLVSRTNMPGR
                     QFIRALVTASFVTPPFLGAVAWELLAAPNSGLLNQLYRVVTGAESDAHLFNIYSMTGI
                     IFVISCYTFPFVFVLVANALDNMPGELEDASAILGGKAWTTARRVTIPLALPALVAGA
                     LIAFLQAMTLFGSPAILALPAGFHTMTTKIWSLFQYPPKLELAAAAAVPLLLLTILLL
                     QAQKFILGRRGYSVVGGKYGAPRRVEMKKWRWAALAFCLVLLLNPVFLPYLALLNGAL
                     SPNATTLVTPSTLTMHNIVFVFTELSSTQLALKNTVILGTATATIGTILALVIAYVTT
                     RRAIAGHRVLGFLATAPVAVPGIVLGVGLFLSYTRPPFVLYGTLWILLIAFLTINLPS
                     AYQQLQAAFATIHPELEDASRILGATRLRSLLQITAPLLRTGVIATWCFIFIGVMREL
                     SAAIVLFTSQTKVLSVLIYDLNESGDLAAISVLGIAMLIITFAVVLAVNQIPVFGGGA
                     GARLRN"
     gene            complement(2389..3450)
                     /locus_tag="VN968_17665"
     CDS             complement(2389..3450)
                     /locus_tag="VN968_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXL31096.1"
                     /translation="MRKRISRRDLIKGSAALGLTAFAAPLKAAAPEPVAITPGLIEAA
                     RKEAKVILYSSMDLPVGEKLGKAFEARYPGIAVQIERSGSERLFQRLDQEFASGIRAA
                     DIVNSSDASHFISWKKNGWLAPFVTEDIARHFLPDYRDPDGMFATSRIWLSSIAYNTN
                     LVKPEDAPQSFTDLLDPKWAGKLVKGHPAYSGTIMTATFQMVRELGWDYMEKLSRQRV
                     MQVQSSTDPPKKLSLGERAVMADGNEYGIVLLKEAGQPVEPVYPIEGAPTISGPTGIF
                     ATAPHPNAARLFQAWLHTREIQQFFIDFTAQYSVHAQVEAKPGRRKISDIKLMKEDAA
                     GVEKMTEEIKTRYAKLFRV"
     gene            complement(3469..3606)
                     /locus_tag="VN968_17670"
     CDS             complement(3469..3606)
                     /locus_tag="VN968_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31097.1"
                     /translation="MKQRKLSRRAGRKPLAEIRLMKEDAEGAEKQSEEIKARYQKLFH
                     V"
     gene            complement(3629..4690)
                     /locus_tag="VN968_17675"
     CDS             complement(3629..4690)
                     /locus_tag="VN968_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31098.1"
                     /translation="MASVELRGLTKRFGSLAVVDDVSLRIDHGQLVCLLGPSGCGKTT
                     TLRLIAGFLEPTDGEIHVGDRLVSSKARTLPPEQRKMSMIFQSYALWPHMTVAENIVY
                     GLRLRKIDRATIAGKFEVILKATKLEILAQRYPGELSGGQQQRVALARALIVEPETLL
                     LDEPLSNLDANLREEMRFEIRRLHDEYRYTTVYVTHDQSEAMTTADLIAVMNSGKIDQ
                     LGTPEDIYDRPQSEFVARFIGASNVIKGTAADENHVAFAGATLQVVGAKLKPGQSAAV
                     AIRQHDIQLSTQAPQAPGNTVKATVTRQVFLGASRDYMVETADGTTLRVVTQTENAVP
                     AGTEVWLTLPPNRCRALSR"
     gene            complement(4914..6101)
                     /locus_tag="VN968_17680"
     CDS             complement(4914..6101)
                     /locus_tag="VN968_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXL31099.1"
                     /translation="MTVRAGREFLAIPGPTTMPDEVLRAMHRPALDIYSDQMLQMTDS
                     LLRDLSRLFATKGHSYIYIANGHGAWEAALSNVLSRGDKVLVLESGRFAIGWGNAAAA
                     MGAEVEVLKGDWRRAIRPAEVEARLRQDRDHSIKAILAVQVDTASGAINDIEAIGKAI
                     KAAGHPALFMVDAVASLGCMPFEMDAWGIDVAMSGSQKGLMAPPGLGFVAANDRARHV
                     HKTAGLRTPYWDWTEREGEEHYRKYAGTAPVHLLFALRQAIDMLFDEQLPNVFQRHRL
                     LAEAVRRAVAVWAEGQVLGFNIAEPGERSDTVTTVVMGGGHDPAALHRFCKEKCGVVL
                     GVGIGELQGQAFRIAHMGHVNAPMILGTLGVVEVGLNALDIPHGKGGTEAAIDWLGES
                     VGA"
     gene            complement(6288..>6860)
                     /locus_tag="VN968_17685"
     CDS             complement(6288..>6860)
                     /locus_tag="VN968_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HXL31100.1"
                     /translation="DQLCLNHTGERWTCGVAARDELIKHADNKTWTCHTRQVVDRRGR
                     TVARCEVDGADIQKWMVRNGWALSYVRFSHDYDDDEKAAREAKAGMWQGAFIAPWDWR
                     ARNKKTAILGAAKPVENANTILLASASGSVAPSPDCTIKGNVNRAGECIYHQPTSRWY
                     AKIKMQISKGTRWFCSVDEAEAAGCRETKR"
BASE COUNT         1215 a         2112 c         2200 g         1333 t
ORIGIN      
        1 attcatggtc ggccgcaagt tcgtcgccat cgagcacgat ctcgatctcg ccgccgtcgc
       61 gctgggtacg gccaaacatc tcatgctcgc cgccgtcgcg gaatttgcgc aaatacgcat
      121 aagcgccgtc cggcgcgggc acgctggaat cgtcctcctt gatccggccg cgcatttctt
      181 tcaccagcgt cttttgcagg cccgcggtgt ggccgagcag gctctcggta tagtcatttt
      241 cggcttcgag atagtttcgg atatccgcgt ccagacctga gggatcgcgc agcacttcct
      301 gccagttggc atccttcagc cacgcataat cgtcgacaac ggtgatgccg tgggtggtga
      361 atgcatgcgg ccggcgcggc gcggagggtg cgggccgtga attctttgta tcgattggtt
      421 ttgtcacttg ggtcctcgtt cgcgggcgga caccgccgca gcccccaata tcggttcgat
      481 ccggtggatt gccagagggg ccgcgaagcg cggcagagtt aattgcgcag ccgcgcgccg
      541 gcgccgccgc cgaacacagg aatctggttg acggccagca ccaccgcgaa ggtgatgatc
      601 agcatcgcga tcccgagcac cgaaatcgcg gcgaggtcgc cgctttcgtt gaggtcgtag
      661 atcagcacgc tgagcacctt ggtctgcgac gtgaacagca cgatcgcggc ggacagttcg
      721 cgcatcacgc cgatgaaaat aaaacaccag gtcgcgatca cgccggtgcg caagagcggc
      781 gcggtgatct ggagcaggga ccgcaaccgc gtcgcgccga gaatccggct ggcatcctcg
      841 agttcgggat ggatggtggc gaaggccgcc tgcaattgct gatacgccga cggcaggttg
      901 atagtcagga acgcgatcag caggatccac agcgtgccat acaagacgaa tggcggccgg
      961 gtgtagctca ggaacaagcc gacaccgagc acgatgccgg gaacggcgac cggcgcggtg
     1021 gcgagaaagc cgagcacgcg atgtcccgcg atcgcgcggc gcgtggtgac gtaggcgatg
     1081 accagcgcca gaatggtgcc gatggtcgcg gtcgccgtgc cgaggatcac cgtgttcttg
     1141 agcgcaagct gcgtcgagga cagttcggta aacacgaaca cgatgttatg catcgtcaac
     1201 gtcgacggcg tgaccagcgt tgtggcgttc ggcgagagcg cgccgttcag cagcgccaga
     1261 tacggcagga acaccggatt gagcagcagc acgaggcaga acgccagcgc cgcccagcgc
     1321 cacttcttca tttcgacgcg gcgcggcgcg ccatatttgc cgccaaccac cgaatagccg
     1381 cgccggccca gaatgaactt ttgggcctgc agcagcagga tggtcaagag cagcagcggc
     1441 accgcggcgg cggcggcaag ttcgagtttg ggcgggtact ggaacaggct ccagatcttc
     1501 gtggtcatgg tgtggaagcc ggccggcagc gccaggatcg cgggcgaacc gaacagcgtc
     1561 atggcctgca ggaaggcgat cagcgcgccg gcgaccagag cagggagcgc cagcgggatc
     1621 gtgacgcgcc gcgccgtggt ccaggccttg ccgccgagaa tggcggaagc atcttcgagt
     1681 tcgcccggca tgttatccag cgcgttggcg acgagaacga acacaaacgg aaacgtatag
     1741 caggagatga caaagatgat cccggtcatc gagtagatgt tgaacagatg cgcgtcggat
     1801 tccgcgccgg tgacaacacg atagagctgg ttgagcaatc cgctgttggg cgccgccagc
     1861 aattcccagg ccaccgcgcc gagaaacggc ggcgtcacga acgaggccgt gacgagcgcg
     1921 cggatgaatt gccgcccggg catgttggtg cgcgacacca gccatcccat cggcgccgcg
     1981 acgaggcagc aaatcgtcgc cgaggtggtg gcgataatcg cggtggtcag cagcggatcg
     2041 aggaaatccg ggtcggtgaa cagcgtgacg aagttctgca aggtcaggtg attggccttg
     2101 tcgatgacgc tgtagatccc gagccacgac agcggcagca cgatcagcag gatcaggcac
     2161 accgcaaaca gcgccagcac cggtttggtc cagtcgatct tgcctttggt ggcgtcgagg
     2221 ccagcggtga tggtcatgtt gtttgccaat ctagagacaa tttcgtcgtt ccggggcgcg
     2281 cggagcgcga gcccggaatc cgtaaccccc gccgggagta tggattccgg gcctgcgcca
     2341 actggcgcat cccggaatga cggtggtgag ttgcttagcc aggcgaatct aaacccgaaa
     2401 caatttcgcg tagcgcgtct tgatttcttc cgtcatcttc tcgacgccag cggcatcttc
     2461 cttcatcagc ttgatgtcgg aaatcttccg ccggcccggc ttcgcctcca cctgcgcatg
     2521 cacggaatac tgcgcggtga agtcgatgaa gaactgctgg atttcccgcg tatgcagcca
     2581 cgcctggaac aaccgcgcag cattggggtg gggcgcggtg gcaaaaattc cggtcggccc
     2641 cgagatcgtc ggcgcgccct cgatcggata caccggctcg accggttgac cggcttcctt
     2701 gagcagcacg atgccgtatt cgttgccgtc ggccatcacg gcgcgttcgc cgagcgaaag
     2761 ttttttcggt ggatcggtcg acgactgcac ctgcatcacg cgctgcctcg acagcttctc
     2821 catgtagtcc cagccgagct cacggaccat ctggaaggtc gcggtcatga tggtgccgct
     2881 ataggccgga tggcccttta ccaatttgcc ggcccatttc ggatcgagca aatccgtaaa
     2941 gctttgcggc gcgtcttcgg gtttcaccag gttggtattg taggcgatgg acgacagcca
     3001 gatccgcgag gtcgcgaaca tgccgtcggg gtcgcgatag tccggcaaaa aatgccgcgc
     3061 gatgtcttcg gtgacgaacg gcgccagcca gccatttttc ttccatgaga tgaagtgcga
     3121 ggcgtccgag ctgttgacga tgtcggcggc gcgaatgccg gaggcaaatt cctgatccag
     3181 ccgctggaac agacgctccg agccggaacg ctcgatctgc acggcaatgc cgggataccg
     3241 cgcctcgaac gccttgccga gcttctcgcc gaccggaaga tccatcgacg aatacagaat
     3301 cacttttgct tctttgcgcg cggcctcgat caggccgggc gtgattgcca ccggctcggg
     3361 tgccgcggcc ttcagcggcg cggcgaacgc ggtgagcccg agcgctgccg agcctttgat
     3421 gaggtcgcgc cgggaaattc tctttcgcat cggtctcacc tttctgcgtc agacgtgaaa
     3481 gagtttttgg taacgcgcct tgatctcttc gctctgcttc tccgcgcctt cggcgtcttc
     3541 cttcatcagc ctgatctcgg cgagcggctt gcggcccgcg cgcctggaaa gttttcgctg
     3601 tttcatcgcg tcctccggct cgtcattgtc atcggctcag cgcccggcag cgattgggcg
     3661 gcagcgtgag ccagacctcg gtacccgccg gaacggcatt ttccgtctgc gtaacgacgc
     3721 gcaaggtcgt gccgtcagct gtttcgacca tatagtcgcg gctggcgccg agaaacactt
     3781 gccgcgtcac ggtggcctta acggtattcc cgggcgcttg cggcgcctgc gtcgaaagct
     3841 ggatatcgtg ctggcggatg gcgaccgccg cactctggcc gggttttagt tttgcaccca
     3901 ccacctgcaa ggtcgcgccc gcgaacgcga catgattttc gtcggccgcg gtgcccttga
     3961 tcacgttgct ggcgccaatg aagcgcgcga cgaattccga ttgcggccgg tcgtaaatat
     4021 cttccggggt gccgagctga tcgatcttcc ccgaattcat caccgcgatc aggtcggctg
     4081 tggtcatcgc ctcggactgg tcgtgggtga cgtacaccgt ggtgtagcgg tattcgtcgt
     4141 gcagccttcg aatttcaaag cgcatctctt cgcgcagatt ggcgtcgaga ttggacaggg
     4201 gctcgtcgag caatagtgtc tccggctcga cgatcagcgc ccgcgccagc gcgacgcgct
     4261 gctgctgtcc gcccgacaat tcgccgggat agcgttgcgc gagaatttcg agcttggtgg
     4321 ctttgaggat cacctcgaat ttcccggcga tggtcgcgcg gtcgatcttc cgcaagcgca
     4381 gcccgtagac gatattttcg gcgaccgtca tgtgcggcca cagcgcgtag ctctgaaaaa
     4441 tcatcgacat cttgcgctgc tcgggcggca gggtgcgcgc cttcgaggac accagccggt
     4501 cgccgacatg gatctcgccg tccgtgggct cgagaaagcc ggcgatcagc cgcagcgtcg
     4561 tggttttgcc gcagcccgag gggcctaaca ggcagaccag ctgcccatgg tcgatccgca
     4621 gcgagacgtc atcgaccacc gcaagcgatc caaaccgctt ggtgaggcca cgcaattcaa
     4681 cggacgccaa tcacatcact ccctatgccg cgccgtcgtc ggcctcttat ttatcgctgg
     4741 atcaatatac gaatatagcg ctgtcgaaaa ggcttcgtac ccttggagtc tgatccagct
     4801 gcattgaggg cattggctcc gtgtctcggc aatggcagtc cgctcccttt tccgttggga
     4861 gagggttgtg tgagggcacg cggcccgacg attgcaggtt cacgaatcta actttaggcg
     4921 cccacacttt cgccgagcca gtcgatcgcg gcctcggtgc cgcccttgcc gtgcgggata
     4981 tcgagcgcat tgaggccgac ttcgacgacg cccagggtgc cgagaatcat cggtgcattg
     5041 acgtgtccca tatgggcgat ccggaatgcc tggccctgga gttcgccgat cccgaccccg
     5101 agcaccacgc cgcatttttc cttgcaaaag cggtgcagcg ccgccggatc gtgaccgcct
     5161 cccatgacaa cggtggtgac cgtgtccgag cgttcgccgg gttcggcgat gttaaagccg
     5221 agcacctggc cttcggccca gaccgcgacc gcgcggcgta ccgcttcggc cagcaggcgg
     5281 tgacgctgga acacgttcgg aagctgctcg tcgaacagca tgtcgatagc ctggcgcaac
     5341 gcgaacagca gatgcaccgg ggcggtgccg gcatatttcc ggtaatgctc ctcgccttcg
     5401 cgctcggtcc agtcccaata tggggtccgc aagccggcgg tcttgtgcac gtgccgggcg
     5461 cggtcgttgg cggcgacgaa gccgaggccg ggcggtgcca tcaggccctt ttgcgagccg
     5521 gacatggcga catcgatgcc ccaggcgtcc atctcgaacg gcatgcagcc gagcgacgcc
     5581 accgcatcga ccatgaacaa agcggggtga ccggcggcct tgatggcctt gccgatggct
     5641 tcgatgtcgt tgatggcgcc ggaggcggtg tcgacctgca ccgccaggat cgccttgatg
     5701 ctgtgatccc tgtcctgccg caggcgggcc tcgacctcgg ccggccgtat cgcgcgccgc
     5761 cagtcgccct tcagcacttc gacctcggcg cccatggcgg cggcggcgtt gccccagccg
     5821 atcgcgaacc ggccgctctc cagcaccagc accttgtcgc cgcgcgacag cacattgctc
     5881 agcgccgctt cccaggcgcc atggccgttg gcgatgtaga tgtaggagtg gcccttggtc
     5941 gcgaacagcc tggagagatc gcgcagcagg ctgtcggtca tctgcagcat ctgatcggaa
     6001 tagatgtcca gcgcaggccg gtgcatcgcc cgcagcactt cgtcgggcat ggtggtgggc
     6061 ccggggatcg ccagaaattc ccggcccgcg cgaacggtca ttgtcggttt tccttgttgg
     6121 tgtgatggcc tgttgcggga tcgaggtgca gcaggacctg cgggtgactt cgatctgggg
     6181 gttttacgca ggttggatgg ctaagaaaag cttccagagc cccggttctg attgaatcat
     6241 acccgaggct ctattttttg ttttgacgtg ttttcgctcg aaacacttta ccgcttggtc
     6301 tcgcggcagc cggcggcttc ggcctcgtcg accgagcaga accagcgcgt gccctttgag
     6361 atttgcatct tgatcttcgc ataccagcgg ctggtgggtt ggtgatagat gcattcgcct
     6421 gcccggttga cgttgccttt aatggtgcag tcgggcgagg gtgcgaccga tcctgacgca
     6481 gacgccagca ggatcgtgtt ggcgttctcg accggcttgg cagccccgag aatggccgtc
     6541 ttcttgttgc gggcccgcca gtcccagggt gcgatgaagg cgccctgcca catccccgcc
     6601 ttggcctcgc gcgcggcttt ctcgtcgtca tcatagtcat gggagaagcg tacataagat
     6661 aacgcccatc cgttcctcac catccatttc tggatgtccg cgccgtcgac ctcgcatctc
     6721 gccacggttc ggccgcgccg gtcgacgacc tgacgggtgt gacaggtcca ggtcttgttg
     6781 tcggcgtgct tgatcagttc gtcgcgcgcg gcgacgccgc aggtccagcg ctcgccggta
     6841 tggttgaggc aaagctggtc
//
LOCUS       DATNOI010000443        19781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1490545,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000443 DATNOI010000000
VERSION     DATNOI010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 19781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19781
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490545"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..316
                     /locus_tag="VN968_17690"
     CDS             2..316
                     /locus_tag="VN968_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXL31101.1"
                     /translation="MRFECTTDLAMVTACNCSICTKKGLHFAFLAPKSFQLRAGEDNL
                     KEYVFNRHAIRHQLCIDCGVDVFARGSKPDGTEVVALNVSCIDGIELAKLTMTPIDGR
                     NR"
     gene            complement(460..1167)
                     /locus_tag="VN968_17695"
     CDS             complement(460..1167)
                     /locus_tag="VN968_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HXL31102.1"
                     /translation="MRYELYYWPGIQGRGEYVRLALEDAGADYVDVARGARGMPAMTG
                     IMEARKGTPPFAPPFLRAGKLLIGQTANILLYLGSRHALAPKAEPGKLWVHQLQLVVA
                     DFVLEVHDTHHPLGPSLYYQDQRAPAKKRTEEFWKQRVPKYLGYFEGILKSNGGFYLS
                     GRRVTYADLSLFQIVEGLRYAFPNRMKGFERRIPGLVDLHDRVANRPNIKAYLASDRR
                     IAFNEDGIFRRYKELDL"
     gene            complement(1379..2074)
                     /locus_tag="VN968_17700"
     CDS             complement(1379..2074)
                     /locus_tag="VN968_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HXL31103.1"
                     /translation="MPTLASNLARACFIAVAAAFAVLAARPAQAQSADTSFFLTSTGI
                     GNGGNLGGLAGADNYCQTLAQAAGANASGVKTWRAYLSTQAVDGQPAVNARDRIGKGP
                     WQNSKGVVIARDVAELHGANNLTKQTALSEKGDVINGRGDTPNRHDVLTGSQADGTAF
                     APGDDRTCKNWTSSTHGSAMVGHSDRIGLRDDDASKSWNSSHPSRGPDGGCSQGDLKS
                     TGGDGLLYCFAAN"
     gene            complement(2262..3215)
                     /locus_tag="VN968_17705"
     CDS             complement(2262..3215)
                     /locus_tag="VN968_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HXL31104.1"
                     /translation="MRFTHRARGIVRAIAVVLSFALPLILAISSADARIGGGMSSGSR
                     GARTFSAPPSTSTAPGTAQPFNRTITQPGSPGLGAPAAAGGGFFNGSGRGLLGGLAAG
                     FLGAGLLGMLFGGGMFSGLGGLSSIFGLILQIGLIIIVVRLAMAWWQRRHTPASAYAG
                     ATPGAAVGPGMFRTGSGFGLGSGSAPLEIAPADYEAFERLLSEIQAAWSNEDVAKLHT
                     LATPEMVSYFTRDLAANKARNEINKVSDLKLLQGDLAEAWREGDTDYASVAMRYALVD
                     KTFDRTTGRLVSGGEPEEVTEVWTFVRPRGMNWELSAIQQT"
     gene            complement(3439..5997)
                     /locus_tag="VN968_17710"
     CDS             complement(3439..5997)
                     /locus_tag="VN968_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXL31105.1"
                     /translation="MTADTDNDPSGTPTTVHEPARRSGSIVLVLLVAGGIVAVAIALM
                     TIGRAQAQPYILALLALLAMVGLFNLFAFAAGIIRFADRAADDPVTGRIADLAYDGLA
                     VTDPRGHVVYSNAAYLALTVAATALDARPVERVFIGNPDVSEAVFRLLKAAREGKRQQ
                     EEVRIAGSDGAHGRWLRMRVRPLGQAKRQAKYAVWSLEDITRDRERQEDVFQELQHAI
                     EYLDHAPCGFFSVNPAGDLVYVNATLANWLDHDLAEIGSGGLKLTDIVSGDGASLLTS
                     IAAVPGEVKTEVFDIDLRMRGGRTMPVRLYHKLAFGANGVPGASRTLVISRARDERAD
                     PQRAAEVRFMRFFDHTPMAIATVDRSGAVVRANARFAKLAQSLSADGAAGKSIFAAVS
                     ARDRNLLIAAINQAAEGQGDIPPVEAMLDGAKERWGQFFVTAVEEDERETEAAIVYLL
                     ETTERRALENQINQSQKMDMVGQLAGGIAHDFNNVLSAIMMANDFLLNAHKPTDPSFQ
                     DIMQIKQNATRAATLVRQLLAFSRRQTLRPQVLDLGDALSDLTMLLRRLIGEKVKLDL
                     VHGRDLWPVKVDVSQFEQVIVNLAVNARDAMPDGGKLTVRTGNVTSEDSGHLAYKGMP
                     AADYVRIDVADTGTGIPAEIVDKIFEPFFSTKEVGKGTGLGLSTVYGIVKQTGGFIYV
                     DSEANQGTSFHIFLPRHHPALEVQPDAAAANGAAKEPTEAKPRTDLTGQGTILLVEDE
                     EGLRSLNARGLRSRGYSVIEASNGIEAMEALEEKNGAVDLVVSDVVMPEMDGPTLLKE
                     MRGRNPDLKIIFVSGYAEDAFEKSLPENQQFAFLPKPFTLSQLVAAVKETMTPL"
     gene            complement(6318..7394)
                     /gene="flhB"
                     /locus_tag="VN968_17715"
     CDS             complement(6318..7394)
                     /gene="flhB"
                     /locus_tag="VN968_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HXL31106.1"
                     /translation="MADDDASEKTEDPTQKRLDDAHDRGDVAKSQEINTWFVIAGATL
                     ILSTFSASIGGGILMPLRNLIANSWMIHADGPGLLALAQSLEYALIAALGVPLLMLSI
                     AAIGGNMIQHRLVWSGEQLKPKFSKVSPGAGFKRVFGKQAVANFAKGIFKVVALGTVM
                     LAVLWPERHRLESMVRFDPATILGATTSLTLQLMGAVVAMLAVVAIADYFFQYRQWYQ
                     RQKMSLQEMKEEFKQSEGDPHVKGRIRQLRHARMKKRMMAGVPKASVIITNPTHYAVA
                     LSYERGMPAPICVAKGIDTIALKIREIAKAHDIPIVENVPLARALHATVEIDHEIPVE
                     HYHAVAQIIGYVMGLKRGVFSRRA"
     gene            complement(7407..8177)
                     /gene="fliR"
                     /locus_tag="VN968_17720"
     CDS             complement(7407..8177)
                     /gene="fliR"
                     /locus_tag="VN968_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HXL31107.1"
                     /translation="MRIDISLLPALAAAFMLVFARIGTMVMLLPGLGESNIPVRVKLG
                     IALLLTLVILPLHRAAYHIDMQSLTPLMVLMVHEIVIGVVLGATARVTLSALQVAGSV
                     IAQQLGLGFVTAIDPTQGQQGLLIGNFLTILGLTLLFATDSHHLVIAALNDSYSIFSP
                     GELMPSGDVAALATRAFAAAFRIGMQLSAPFLVFGLVFNIGLGVLARLMPQMQVYFVG
                     VPLSILAGFLIFALVIAAMMGTFLDYFVGVMHDLIPLS"
     gene            complement(8195..8458)
                     /gene="fliQ"
                     /locus_tag="VN968_17725"
     CDS             complement(8195..8458)
                     /gene="fliQ"
                     /locus_tag="VN968_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HXL31108.1"
                     /translation="MTGAETLDVARDAIWTIVVVSSPLMVVGLVVGVVVSLFQALTQI
                     QEQTLVFVPKILAIFVTLLLALPFMADSLHSHMMRISSRIIGG"
     gene            complement(8592..8903)
                     /gene="fliE"
                     /locus_tag="VN968_17730"
     CDS             complement(8592..8903)
                     /gene="fliE"
                     /locus_tag="VN968_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HXL31109.1"
                     /translation="MASPTVAANAYASLARILDTGGAGKASEGGGGPSFGALLKDAIG
                     SVLDAGKKSDAQTVAMASGKANVMDVVTAVAETDVAVSTLVSVRDRVIQSYEDIMKMP
                     I"
     gene            complement(8921..9346)
                     /gene="flgC"
                     /locus_tag="VN968_17735"
     CDS             complement(8921..9346)
                     /gene="flgC"
                     /locus_tag="VN968_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HXL31110.1"
                     /translation="MADDAGDFARSMSIATSGLRAQAGRMRILSENIANADSTAQTTG
                     GDPYRRKVPTFSSELDRTLDARVVTLGRIRADTSPFRVKYEPANPAADAAGNVKYPNV
                     NSLVEQTDMRDAQRSYEANLNIISATRRMIQRTLDILKA"
     gene            complement(9380..9784)
                     /gene="flgB"
                     /locus_tag="VN968_17740"
     CDS             complement(9380..9784)
                     /gene="flgB"
                     /locus_tag="VN968_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HXL31111.1"
                     /translation="MSINDLPVLSALRTKMQWHQERQRVLSENVANSDTPNFKPRDLV
                     EPKLDRTGGVAGSVGALAMMRTSGSHIGTSAGGPTFAQDGNGGFQTRPAGNAVNLEDQ
                     MLKVSANQMDYAAATSLYSKSLHLLKTAIGKG"
     gene            10157..11119
                     /locus_tag="VN968_17745"
     CDS             10157..11119
                     /locus_tag="VN968_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HXL31112.1"
                     /translation="MQALTFVFAFVAVLALIGVAAWLVRRFAGSRLGTNTNRGRMPRL
                     AVIDAAAVDGRRRLVLVRRDNIEHLLMIGGPTDIVVESNIVRANVARDQMPQRPSVGG
                     GAEPPQPRLVPLPDSAGRGDSDGSRSDGFDHAEPQLPEPPSRPARPSFADEVRRPAPA
                     AAERRGDPLTGFTPEPLAGRAEAHSEPMPLRLPSRNEPMMPRLPRVNVSDTPKPPPLR
                     APERAPVSPPPPPLPAQPTAEQNLAEMAQRLEAALRRPGDTGEARIGAPSVAPEPPPV
                     RLTPPPPRPEQSTAPPVAAPLPKSGFENLEDEMASLLGRPKNPS"
     gene            11116..11868
                     /gene="fliP"
                     /locus_tag="VN968_17750"
     CDS             11116..11868
                     /gene="fliP"
                     /locus_tag="VN968_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614450.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HXL31113.1"
                     /translation="MRPAEVPRRVLFFATLIAAGSLAGPAFAQDISINLGQGNGGVTE
                     RAIQLIALLTVLSIAPSILIMMTSFTRIVVVLSLLRTALGTATAPPNSVIIALAMFLT
                     AFVMGPVLQKSYDDGIKPLVANQINVEEALQRAAVPLRGFMQKNVREKDLKLFMDLSG
                     EAPPATPDEMSLRILVPAFMISELKRAFEIGFLLFLPFLIIDLVVASVLMSMGMMMLP
                     PVVVSLPFKLIFFVLVDGWSLVAGSLVQSYGG"
     gene            11990..12403
                     /locus_tag="VN968_17755"
     CDS             11990..12403
                     /locus_tag="VN968_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31114.1"
                     /translation="MLRLVHLERADELRAEAKEAQKQRVKRLDSTVFGTELMDFQEEE
                     GYISTEGNIMSARFQHITFGSTEAYIRELASLHQVVFTPTKLDAFAQDVTRLAADHVR
                     LDEVECLLLGLQRAGHLSRPELVHLQAKYLREAKR"
     gene            12400..13134
                     /locus_tag="VN968_17760"
     CDS             12400..13134
                     /locus_tag="VN968_17760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HXL31115.1"
                     /translation="MSFDPFGDFATEGYLRNVEKEKDLDIVKRLEHTSFTTGLDEAFT
                     ALGKSRQFSYSDVLRTHGILFNAVYPWAGQDRLSIAPALSVRKGPVIFANPSEIRPAV
                     EFALRKAQEKDYLRAHPGEIMGHLAFGHPFLDGNGRTIVTLFSALAQRAGFSVDWAAT
                     DKDAYLDALTKEIDVPSKGHLDNYLGQFIRKPIAHEELAAQIINAPGIDGRALTGDEN
                     EVIGNVDAPEVKAQYEAMLLKRGKKN"
     gene            13468..13863
                     /locus_tag="VN968_17765"
     CDS             13468..13863
                     /locus_tag="VN968_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HXL31116.1"
                     /translation="MQVASELISLQKRWRNLLVVGAISVLIGAGAHAQNIDQGKSAAR
                     LFADGCATCHRSARGLAKGRFRLTLYLFLQDHYASNSSSAWALASYLESVDSPQRGRS
                     RAAAAKPSRPAVGTSRSSILRPPASVPGR"
     gene            14040..14924
                     /locus_tag="VN968_17770"
     CDS             14040..14924
                     /locus_tag="VN968_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31117.1"
                     /translation="MAFFKRELSPAQRFERALREKQAARQKLADQLSLAETALGEKRD
                     EAERLAVAGAANAKLARAEARMRTFEDRAKTLRAALVEFDEQVVATERALADAKAQRD
                     RDRAADEIEALAAAIEQAAPGFGAGAAALVEAVTKSMASVPEATMFSTSVDEMRREVL
                     SAADLICWELRSTAVRTRAGNANIALLAPPAPKQLPAPEIERQLIYTLSPLLWREGSE
                     VRRVPAFALVGLPKMLLLVALRHQHVDYLNARRVQTLIHVHGSGGVHSDPDQDDPQLV
                     DLDALAAEEKESAQADVA"
     gene            complement(15099..15851)
                     /locus_tag="VN968_17775"
     CDS             complement(15099..15851)
                     /locus_tag="VN968_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HXL31118.1"
                     /translation="MRILVVNPNTTVAMTEKIGRAARAAAASGTEIIAVNPMDGPVSI
                     EGYYDEAFSVPGLLAEIAKGDASGVSAHIIACFDDTGLEAARSVSFAPVIGIGEAAFH
                     LASMLGHRFSVVTTLSRSIAAIENNLLKYGLAGRCGKVRACELPVLSLDDPTSNAAAQ
                     ISAEIELAKQEDQAEVIVLGCAGMAELAARLTEKHELPVIDGVASAVKLAEACGSLGL
                     KTSKIGAYALPRPKSYLGSFASFAPRQGPRDV"
     gene            complement(15862..16740)
                     /locus_tag="VN968_17780"
     CDS             complement(15862..16740)
                     /locus_tag="VN968_17780"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31119.1"
                     /translation="MGENRPRSFYFLAAFFIAYVLFLYGPMFAIYILSFQGPDGGLTF
                     PMNGVSLQWFGKVFSGTGIVDIGAAFKRSLKLGLVVMALTVVVSVSAGLAFRKSFRGA
                     TFLFYTAIASLIMPSIITSLGIALEFRLLDDFMAKHGSEGWTTAMGLFTSGLGAHLTW
                     TLPFGLLIMFAIFNRFDGRLEEAARDLGATPWQTFRHVVLPIILPSVVGIGLFGFTLS
                     WDELARSSQAIGSVNTLPLELQGLTTTVTKPDIYALGTLTSAVSFLVIFSALIVILVL
                     QARQRRHGSDAGKGMV"
     gene            complement(16742..17623)
                     /locus_tag="VN968_17785"
     CDS             complement(16742..17623)
                     /locus_tag="VN968_17785"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31120.1"
                     /translation="MVQERKSFVAWLQAAPMMLIFALFFLLPLAFVTIVSAWDYNEYE
                     MIPAFSLRGYTDTFEGCIADLPDLCTILKTYLKTLKLCLLTWSLTLLIGFTVAYFLAF
                     HVRSKTWQIVLTLLCTIPFWTSNVIRMIAWIPLLGRNGLVNKGLEGVGLIRAPLEWLL
                     FSEFSVVLALVHLFTFFMVVPIFNSMIRIDKRLIEAAYDAGATGWQTLVNVVIPLAKP
                     GIVIGSIFVITIVMGDFVTIGVMGGQQIASAGKIIEARLNALQFPAAAANAVILLGIT
                     ILIISSLTRIVDIRKEL"
     gene            complement(17694..18992)
                     /locus_tag="VN968_17790"
     CDS             complement(17694..18992)
                     /locus_tag="VN968_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXL31121.1"
                     /translation="MTMSNRWKFNSSVSRRAVLKGAAGTVGLAAGSGAITGFPYVHSA
                     EPKVLRYLGTAVNEGDDISKKCLEDTGIKIEYITATTDDVTKRVITQPNSFDVLDTEY
                     FSLKKLVPSGNIFALDAKKIKEFDNITPVFTKGQLPNGKKIGDQGTAPWKVLYLEGPN
                     SKKFSTTPTEFVTLIPTVYNADTLGIRPDLIKRPIASWTELLNPEFKGKASILNIPSI
                     GIMDAAMVVEATGQHKYADKGNMTKAEIDLTMKIMTEAKRAGQFRAFWKDFNESVNLM
                     ASGETVIQSMWSPAVTKVRSMGIACTFQPLKEGYRSWASGFCVSKAVSGPKLDWAYEF
                     VNWYLSGWAGAYLNRQGYYSAVLSTAKANMTPDEWGYWMEGKAAVGDVKAPDGTVLEK
                     AGAKRDGGSYDDRMGAVACWNAVMDENDYMVRKWNEFIAA"
     gene            complement(19004..>19781)
                     /locus_tag="VN968_17795"
     CDS             complement(19004..>19781)
                     /locus_tag="VN968_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31122.1"
                     /translation="YALFPHLSVIDNVAFALKMKGIAKAERHAEAQKLLQLVDMQAYA
                     ARLPGQLSGGQQQRVALARALITSPQILLLDEPLSALDPFLRLRMRAELKKLQRELGI
                     SFIHVTHGQDEAMALADIVVLMNGGRIEQQGSPREIYNHPRTEFTAKFIGGHNVVAVD
                     DETFAVRNDRLMLKKPSEAVNGPSIAGTVSEVEYQGTYVRVAIAADGGADISAQLTES
                     QFDAANYSVGERVLATWDPALASPLKTRSSVTVPSPEKAA"
BASE COUNT         3827 a         6060 c         6133 g         3761 t
ORIGIN      
        1 ggtgcgtttt gaatgcacca ccgatcttgc gatggtgacc gcctgcaact gctcgatctg
       61 caccaagaag ggcctgcatt tcgcgttcct ggcgccaaag agttttcagc tccgcgccgg
      121 cgaggacaat ctgaaggaat atgtgttcaa caggcacgcg atccggcatc agctctgcat
      181 cgactgcggc gtcgacgtgt tcgcgcgagg cagcaagccc gacggcaccg aagtggtggc
      241 gctcaatgtc agctgcatcg acggcatcga gctggcaaaa ctcacgatga cgccgatcga
      301 cgggcggaac aggtagcagc acaacaccgt cattccacga tgtgcaattg cacatcgtgg
      361 atgcgccatt tggcgcaggc ccggaatcca tactcccggt cgtggtcatg gattccgggc
      421 tcgctcgttc ctcgcgcccc ggaataacga aaaatgtgcc tagagatcca gttccttgta
      481 gcgacgaaaa atgccgtcct cgttgaaggc gatgcggcgg tcgctggcga gataggcctt
      541 gatgttcggt ctgttggcga cgcggtcatg cagatcgacg agaccgggga tccgccgctc
      601 gaaccctttc atgcggttgg gaaatgcgta gcgcagccct tccacgatct ggaaaagcga
      661 tagatcagca taggtcacgc ggcgccccga gagatagaag ccgccattgc tcttcagtat
      721 tccctcgaaa tagccaaggt atttcggcac gcgttgtttc cagaattcct cggtgcgttt
      781 tttcgcaggt gcacgctggt cctggtaata tagcgacggg ccgagcggat ggtgggtatc
      841 gtgaacttcc agcacgaagt cggcgacgac gagctgcagc tgatgcaccc atagctttcc
      901 tggctccgcc ttcggcgcca gagcatggcg cgatccgaga tagagcagga tattggcggt
      961 ctgcccgatc aggagtttcc ctgctctaag aaacggcggc gcgaacggcg gcgtgccctt
     1021 gcgcgcctcc atgatcccgg tcatcgcggg cataccgcga gcgccgcgcg cgacatcgac
     1081 atagtcggcg ccggcatcct ccagcgcgag ccggacgtat tcgccacggc cctgaatgcc
     1141 cggccagtaa tagagttcat agcgcatgga gaatccttcg atcggagtct gactcgaaat
     1201 acagccgata gaattaggtc gtatttgtcg tcatggcgag ggccgaagcg acgaagcaaa
     1261 tccacactcg gtcattccgg ggcgatgcga agcatcgaac ccggaagctc gtcatcgggc
     1321 gcgccttcgc gcgacccgtt ggcgcggacc ggttggtatt gccccggaat gatttacatc
     1381 agttcgccgc aaaacaatac agcagcccgt cgccgccggt gctcttgaga tctccttgcg
     1441 agcagccgcc atcgggtcca cgcgagggat gcgagctgtt ccaggatttc gacgcgtcgt
     1501 catcgcgcag tccgatgcgg tcggagtgac cgaccatcgc cgagccgtgc gtgctgctcg
     1561 tccagttctt gcaggtgcgg tcgtcaccgg gcgcgaacgc cgtaccgtcg gcttgcgatc
     1621 ccgtcagcac gtcatggcgg ttcggcgtat cgccgcggcc gttgatgacg tcgcccttct
     1681 cgctcagcgc ggtctgtttg gtcaggttgt tggcgccgtg cagctcggcg acatccctgg
     1741 cgatcacgac gcctttggaa ttctgccagg gtcccttgcc gatgcggtcg cgcgcattca
     1801 cggctggctg accatcgaca gcctgggtcg agagataggc gcgccaggtt tttacccccg
     1861 acgcgttggc gccggccgcc tgcgccaggg tctgacagta attgtcggcg ccggcgagac
     1921 cgccgaggtt gccgccattg ccgatgccgg tcgaggtcag gaagaaactg gtgtcggccg
     1981 attgcgcctg cgccggtcga gccgcgagaa ccgcgaaggc cgccgcgacc gcgatgaagc
     2041 acgcgcgcgc gagattgctg gcgagagtgg gcatctcatc ctcctgtctt tttgatctgc
     2101 cggcgcgttg ctgcgccggc ttggtctgat accaacacgt tttgggagat attattccgt
     2161 tcccgcgaca tccagacgaa gacggcaagg cctgacaaaa tcaaaaggcg ccgcgggttg
     2221 aaccgcggcg ccttgcatcg agatgatcgg ttcgcagacg atcaggtctg ctggatcgcc
     2281 gagagttccc agttcatgcc gcgcggccgc acgaaggtcc acacttccgt gacctcctcc
     2341 ggctcgccac ccgagaccag ccggccggtg gtgcggtcga aggtcttgtc gaccagcgcg
     2401 taccgcatcg caacgctggc gtaatccgta tcgccttcgc gccaggcttc cgcgaggtcg
     2461 ccctgcagca gcttgaggtc ggagaccttg ttgatttcgt tgcgcgcctt gttcgccgcc
     2521 agatcccggg tgaaatacga caccatttcg ggcgtcgcca gcgtgtgcag tttggcgaca
     2581 tcctcattcg accacgccgc ctgaatctcg ctgagcaggc gctcgaaggc ttcgtaatcg
     2641 gccggagcga tctcgagcgg ggcgctgccc gatcccaatc cgaaaccgct tccggtccgg
     2701 aacatgccgg ggccaaccgc ggcgccaggg gtcgcgcccg cataggccga tgccggcgtg
     2761 tgccggcgct gccaccatgc catcgccagt cggaccacga tgatgatcaa gccgatctgc
     2821 aggatcaggc cgaagatcga cgacaatccg ccgagcccgg agaacatgcc gccgccgaac
     2881 aacatgccga gcaggccggc gccaaggaag cccgcggcga gaccgccgag caggcctcgg
     2941 ccggacccgt tgaagaaccc gccgccggcg gccgcaggtg cgcccaggcc ggggctgccc
     3001 ggctgggtga tggtgcgatt gaacggctgt gcggtaccgg gtgccgtcga ggtgctcggc
     3061 ggtgcggaga acgtccgcgc tccccgggaa cctgaactca tgccgccgcc gatccgggcg
     3121 tcggccgacg agatcgctaa tatcaatggt aaagcgaatg ataaaaccac ggcaatggcc
     3181 cggacaatgc cgcgtgcgcg atgcgtgaat ctcatggcaa tttcccttaa aatccccggc
     3241 agggggaagc gctattaaga tgggcagcgc tgcctaaaag tgaagccgct atcgtgattt
     3301 gcggctgcgg ccctcggtcg cgggcatgcg acgatagcgc tcgttctcgg ccgcttggat
     3361 tttggggatg ttgtggatcc cgactctgcg gtgcaccgtg tagaacaact gcaccgcgtc
     3421 cgggacacgc acaggacttc ataacggcgt catggtttcc ttcaccgccg ccaccaattg
     3481 gctgagcgtg aaaggttttg gcaggaacgc gaactgctga ttttcgggca ggctcttctc
     3541 gaaggcatct tccgcatagc cggacacgaa gatgatcttg aggtccggat tgcggccgcg
     3601 catctccttg agcagggtcg ggccatccat ttccggcatc accacgtcgg aaaccacgag
     3661 atccaccgca ccgttcttct cctccagcgc ttccatcgcc tcgatgccgt tcgacgcctc
     3721 gatcacgctg tagccgcgcg agcgcaggcc gcgcgcgttg agggagcgca ggccttcctc
     3781 gtcttcaacc agcagaatgg tgccctgccc ggtgagatcg gtccgcggct tggcttcggt
     3841 cggctccttg gccgcgccgt tggcggccgc ggcatcgggc tgaacctcca gcgcaggatg
     3901 gtgccgcggt aaaaagatgt ggaacgaggt gccctggttc gcctcggagt ccacatagat
     3961 gaagccgccg gtttgcttga cgatgccata gaccgtcgac aggccgaggc cggtgccctt
     4021 gccgacttcc ttggtcgaga agaacggctc gaagatcttg tcgacgatct cggccggaat
     4081 tcccgtgccg gtgtccgcga cgtcgatccg cacataatcc gccgccggca tgcccttata
     4141 ggcgagatgc cctgaatcct ccgacgtcac attgccggtt cgcaccgtga gcttgccgcc
     4201 gtcgggcatc gcatcgcggg cgttaacggc cagattgacg atgacctgtt cgaattgcga
     4261 gacgtcgacc ttgaccggcc agagatcgcg gccatgcacc agatcgagtt tcaccttttc
     4321 gccgatcagg cggcgcagca gcatggtgag atcgctcaat gcgtcgccga gatcgagcac
     4381 ctgcggccgc agggtctggc ggcgcgagaa cgccagcagc tgacgcacca gcgtcgccgc
     4441 gcgggtcgcg ttctgcttga tctgcatgat atcctggaac gagggatcgg tcggcttgtg
     4501 cgcgttcagc agaaaatcgt tcgccatcat gatggcggag agcacgttgt tgaaatcatg
     4561 cgcgatgccg ccggcgagct ggccgaccat gtccatcttc tgcgactggt tgatctggtt
     4621 ctcgagcgcg cgccgttcgg tggtctccag gagatagacg atggcggcct cggtctcgcg
     4681 ctcgtcttcc tcgaccgcgg tcacgaagaa ctgtccccat cgctccttgg cgccgtcgag
     4741 catcgcctcg accggcggga tatcgccctg cccctcggcg gcctggttga tggccgcgat
     4801 cagcaggttg cgatcgcggg cgctgaccgc cgcgaaaatc gacttgccgg ccgcgccgtc
     4861 ggcgctcagg ctttgcgcca gcttggcgaa gcgggcattg gcgcggacca ccgcgcccga
     4921 ccggtccacg gtcgcgatcg ccatcggggt gtgatcgaag aagcgcatga agcggacttc
     4981 ggcggcgcgc tgcggatcgg cgcgttcgtc gcgcgcgcgg ctgatcacca gcgtgcgcga
     5041 cgcgccgggc accccgttcg cgccgaacgc cagcttatga tagagccgca ccggcatggt
     5101 cctgccgccg cgcatgcgca gatcgatgtc gaatacctcg gtcttgacct cgccgggcac
     5161 cgccgcgatc gacgtcagca acgaagcgcc gtcgccggac acgatgtcgg tcagcttcag
     5221 accgcccgac ccgatttcgg ccagatcgtg atccagccag ttcgccagcg tggcattgac
     5281 atagaccagg tctccggccg gattgaccga gaagaacccg cacggcgcat gatcgagata
     5341 ttcgatcgcg tgctgcagtt cctgaaacac gtcctcctgc cgctcgcggt cgcgggtgat
     5401 gtcctccagc gaccacaccg cgtatttggc ctggcgcttg gcctggccga gcggccgaac
     5461 ccgcatccgc agccagcggc cgtgcgcgcc atcggatcct gcaatgcgga cctcttcctg
     5521 ctgccgcttg ccttcgcgcg ccgccttgag caggcgaaac accgcctcgg acacgtcggg
     5581 attgccgatg aagacccgct cgaccggacg cgcgtcgagc gcggtcgcag ccaccgtgag
     5641 cgcgagatag gccgcattgg aatagaccac atgaccgcgg gggtcggtga ccgccagccc
     5701 gtcataggcc agatcggcga tgcgacccgt caccggatcg tcggcggcac ggtcggcgaa
     5761 acggataatg ccggcggcga aggcgaacag gttgaacagc ccgaccatcg ccaaaagcgc
     5821 cagcagcgcc aggatataag gctgcgcctg ggcgcgcccg atggtcatca gggcgatcgc
     5881 cacggcgacg atcccgccgg ccaccagcag caccagaacg atgctgccgc tgcgccgcgc
     5941 cggctcatgg acggttgtcg gcgttcctga cgggtcgttg tcagtatcgg cggtcatacg
     6001 agggacgcgg cctgtcgaca tgaatatgac gcgctaatgg cgcgcccgct cctgagtgcc
     6061 tgaatcggcc cggcgagcgc aagtccatcg ggcggctatt tcgtgcgttc ccccgtgttt
     6121 ccagcaggtt ttgcgcaaaa tagcccgctc ccgatgtgac ggtgggctgc gttctcttca
     6181 ttgtcgcggc tggtccgtcg cgagaagttc tgaacctaaa ccacgctggg atcacaggtt
     6241 tccggcatcc tcagcgatcc gaaggtcgcc gatccgaagg tcgctgaagc gggcgctgcc
     6301 aatggtcacg accgggttca cgcccggcgg ctgaagaccc cgcgcttgag acccatcacg
     6361 tagccgataa tttgcgcgac cgcatgatag tgctccaccg ggatctcgtg gtcgatctcc
     6421 acggtggcgt gcaaggccct cgccagtggc acgttttcga cgatcgggat atcgtgcgcc
     6481 ttggcgatct ccctgatctt gagcgcgatg gtatcgatgc ccttggcgac gcagatcggc
     6541 gccggcatgc cgcgttcgta actcagcgcc accgcgtagt gggtcgggtt ggtgataatc
     6601 accgaggcct tgggaacccc ggccatcatg cgcttcttca tgcgcgcgtg ccgcaactgc
     6661 ctgatccggc ccttgacgtg cgggtcgcct tcggattgct tgaattcttc cttcatttcc
     6721 tgcagcgaca tcttctgtcg ctggtaccat tgccggtact ggaagaagta gtccgcgatc
     6781 gcgacaaccg cgagcatcgc cacaacggcg cccatcagtt gcagcgtcag gctggtggtc
     6841 gcgcccaaaa tcgtcgccgg gtcgaaccgt accatcgatt ccagacgatg gcgctccggc
     6901 caaagcaccg ccagcatcac cgtgcccagc gccactactt taaagatgcc cttggcgaag
     6961 ttcgccaccg cctgcttgcc gaagacccgc ttgaacccgg ccccgggcga gaccttgctg
     7021 aatttcggct tgagctgttc gcccgaccac accagccggt gctgtatcat gttgcctccg
     7081 atcgccgcga tcgacagcat caaaagcggc acgccgaggg ccgcgatcag cgcatattcg
     7141 aggctctgcg ccagcgccag caggccgggc ccgtcggcgt ggatcatcca ggaattggcg
     7201 atcaggttgc gcagcggcat caggatcccc ccgccgatcg atgcggaaaa cgtcgacaga
     7261 atcagtgtcg caccggcaat cacgaaccag gtgttgattt cctggctttt ggcgacatcg
     7321 ccgcgatcat gggcatcatc cagacgtttt tgcgtcgggt cttctgtttt ctcggatgcg
     7381 tcgtcgtcgg ccatggttcc aggctttcag ctgagcggga tcaggtcgtg catgacgcca
     7441 acaaaataat cgaggaacgt tcccatcatc gccgcgataa cgagggcgaa aatgaggaat
     7501 ccggcaagaa tcgatagcgg caccccgacg aaataaactt gcatttgcgg catcagccgc
     7561 gccagcacgc caagcccgat attgaaaacc aggccaaaaa ccagaaacgg cgccgaaagc
     7621 tgcatgccga tccgaaacgc ggcggcgaag gcgcgggtgg cgagcgccgc gacgtcgccg
     7681 ctcggcatca attctcctgg cgaaaagatc gaatagctgt cgttgagggc ggcgatgacc
     7741 agatggtggc tgtcggtcgc aaacaatagc gtcaggccga ggatggtgag gaaatttccg
     7801 atcaagaggc cctgctgacc ctgggtcgga tcgatcgcgg tgacaaatcc gaggccgagt
     7861 tgctgggcga tcaccgagcc tgcaacctgc agcgccgaaa gcgtgacccg cgcggtggcg
     7921 ccgagcacga cgccgatgac gatctcgtga accatcaaga ccatcagcgg cgtgagcgac
     7981 tgcatgtcga tgtgataggc ggcgcggtgc agcggcagaa tcaccagcgt cagcaacagc
     8041 gcgatcccaa gcttgacccg caccggaata ttgctctcgc ccagccccgg caacagcatg
     8101 accatcgtgc cgatgcgggc gaataccagc atgaaggcgg cggcgagcgc gggcagcagt
     8161 gaaatatcga tgcgcatgat tcgggcatac tgcatcaacc gccaatgatt cgcgacgata
     8221 tccgcatcat atggctgtgt agcgagtccg ccataaatgg cagcgccagc agcagggtaa
     8281 cgaagatcgc caggattttc ggcacgaaca ccagcgtttg ttcctggatc tgggtcagcg
     8341 cctggaacag cgacaccacg acaccgacca caaggccgac aaccatgagt ggcgacgaca
     8401 ccacgacgat ggtccagatc gcatcgcgcg ccacgtcgag ggtttcggca ccggtcattt
     8461 ttctgtctcg ttctatttga tttgcgttcg tcattccggg gcagtccgca ggactgaacc
     8521 cggaatctcg agattccccg atgtgcaatt gcacatccga ggctcgcgcc tcgcgcgccc
     8581 cggaatgacg atcagatcgg catcttcatg atgtcttcgt aggactggat gacgcggtcg
     8641 cgcaccgaca ccagggtcga gaccgcgacg tcggtttccg ccaccgccgt caccacgtcc
     8701 atgacgttgg ccttgcccga cgccatcgcc accgtctgcg cgtcggattt ttttccggcg
     8761 tccaggacgc ttccgatcgc gtccttgagc agcgccccga aggatggacc gccgccgcct
     8821 tcggaagcct tgccggcgcc gccggtgtcc aggatgcggg cgaggctggc ataggcattg
     8881 gcagcgactg tgggtgaagc catggcttaa ggttccctgt tcaggccttg aggatgtcga
     8941 gcgtgcgttg aatcatccgg cgcgtggcgc tgatgatgtt gaggttcgcc tcgtaggacc
     9001 gctgcgcgtc gcgcatgtcg gtctgttcaa ccagcgagtt gacgttggga tatttgacgt
     9061 tgccggcggc atccgcggcc ggattggcgg gctcgtattt gacgcgaaac ggcgacgtat
     9121 cggcccggat cctgcccagg gtcacgaccc tggcgtccag ggtgcggtcg agttcggagg
     9181 aaaacgtcgg caccttgcgg cgatagggat cgccgccggt ggtctgcgcg gtggaatccg
     9241 cattggcgat attttccgac aggatccgca tgcgcccggc ctgcgcccgc aggccggacg
     9301 tggcgatgct catcgacctc gcgaaatccc ctgcatcgtc tgccatggtg tggttccctt
     9361 aggtctttga cccggcctgt cagcctttgc cgatcgcggt cttcagaaga tgcaggcttt
     9421 tgctgtagag cgaagtcgcc gccgcatagt ccatctggtt ggcggagacc ttgagcatct
     9481 ggtcttccag attgacggcg ttgccggcgg gcctggtctg gaaaccgccg ttgccgtcct
     9541 gcgcaaaagt tggtccgccc gccgaggtgc cgatatggct gccgctggtg cgcatcatgg
     9601 ccagcgcgcc caccgatccg gcgacgccgc cggtgcgatc gagtttcggt tccaccagat
     9661 cgcgcggctt gaaattcggc gtgtcggaat tggcgacgtt ttcggacagc acgcgctgcc
     9721 gctcctgatg ccattgcatc ttggtgcgca gcgccgacaa aaccggaaga tcgttgatgg
     9781 acatcgcatc ggcctctcgc cttgcggcga tggcgccgct gaggtaggca gaatttgccc
     9841 tgcgcatggt taataacggg ttaagatgcg cggcaggccg gacggcgcgc gggcgtgaat
     9901 cggcgaccaa gatcaatcga gcgcattttc gccacgaatg ctgcgttaac cagccaaatc
     9961 agcgcagcgg gaacgctcag aagtcgtttt ggccacagcg attcttggtt tattaggaca
    10021 tggggcgcgg caattcttgc cgtgggacgt taagacatag ggccaatctc acgctttttg
    10081 ccgttttctg catcggttcc gatccacggc cgacgaacag aaacctcaac aaagcgttat
    10141 ccgccgggaa caaagcatgc aggcacttac attcgttttc gcgttcgtcg ccgttctggc
    10201 gctgattggc gttgccgcct ggctggttcg ccgattcgcc ggcagccggc tcggtaccaa
    10261 caccaatcgc gggcggatgc cgcggctggc ggtgatcgac gccgccgccg tggatggccg
    10321 ccggcggctg gtgctggtcc ggcgcgacaa catcgaacat ctgctgatga tcggcggacc
    10381 gaccgatatc gtcgtggaat ccaatatcgt acgcgccaac gtcgcacggg atcagatgcc
    10441 gcaacggcct tctgtcgggg gaggcgccga accaccgcaa ccccggctgg tgccgctgcc
    10501 ggattcagcc ggccgcggcg acagcgatgg atcgcggtcc gatggcttcg atcacgctga
    10561 gccgcaactg cccgagccgc cttcccggcc ggcgcggcct tcgtttgccg acgaagtgcg
    10621 ccgtccagca cctgcggccg cggaacggcg cggcgacccc ttgaccggct tcacgccgga
    10681 gccgctggcc ggccgcgccg aggcgcacag cgagccgatg ccgttgcggc tgccatcgcg
    10741 caacgaaccg atgatgcccc gcctgccccg cgtgaacgtg agcgacacgc caaagccgcc
    10801 cccgcttcgc gcgcccgaac gcgccccggt atcgccgccg ccaccgccgc tcccggctca
    10861 gccgaccgcc gaacaaaatc tcgccgagat ggcacagcgg ctggaggcag cattgcgccg
    10921 gcccggcgac accggcgaag cgcgcatcgg cgcgccgtcg gtggcgcccg aaccgccgcc
    10981 ggtccggctc accccgcccc cgccgcgccc cgagcaatcg acggcgccgc cggtcgccgc
    11041 gccattgccg aagagcggtt ttgaaaatct cgaagacgaa atggcgtcat tgctgggccg
    11101 tccgaagaat ccttcgtgag gccggcagaa gtcccgcgta gagttctatt ttttgccacc
    11161 ctgatagccg ccggatcgct ggcgggtccg gcgttcgcgc aggacatcag catcaatctc
    11221 ggccagggca atggcggcgt caccgagcgc gcgatccagc tgatcgcgct cctgacggtg
    11281 ctgtcgatcg caccgtcgat cctgatcatg atgacgtcgt tcaccaggat cgtggtggtg
    11341 ctgtcgctgc tgcggaccgc attgggcacc gcgaccgcgc cgccgaattc cgttatcatt
    11401 gcgctggcga tgttcctgac cgcgttcgtg atgggaccgg tgctgcagaa atcctacgac
    11461 gacggcatca agccgctggt cgccaaccag atcaacgtcg aggaggcgct gcagcgggcg
    11521 gcggtgccgc tgcgcggctt catgcagaag aacgtgcgcg aaaaggacct aaaactgttc
    11581 atggacctgt cgggcgaggc accgccggcg acccctgacg aaatgtcgct gcggattctg
    11641 gtgccagcgt tcatgatctc cgaactgaag cgcgccttcg agatcggctt cctgttgttc
    11701 ctgccgtttc tgatcatcga cctcgtggtc gcctcggtgc tgatgtcgat gggcatgatg
    11761 atgctgccgc cggtggtggt gtcgctgccg ttcaagctga tcttcttcgt gctggtcgac
    11821 ggctggtcgc tggtagcggg cagcttggtg cagagctacg ggggataggc tggcggactg
    11881 cgttgctgtc cgctgtttgg agcaagaccg acttcgtcga ggaggtttcc acctcaaagt
    11941 ttgatttgcg gcggattgat cggttaaagc gccacctctt cgcgcgagca tgctgcgctt
    12001 ggtacatttg gagcgggctg acgaactccg cgccgaggct aaagaagcac aaaaacaaag
    12061 agttaagagg ctggattcga ctgttttcgg gacggaatta atggattttc aggaggagga
    12121 aggctatatt tccactgagg gaaacatcat gtcggcacgg ttccaacaca tcactttcgg
    12181 ctcgaccgaa gcctatatcc gcgagctcgc gtcactgcac caagttgttt tcaccccgac
    12241 aaagcttgac gcgttcgcgc aggatgtcac ccgtcttgcg gcggatcatg tgcgcctcga
    12301 tgaagtggaa tgcctgcttc tcggcttaca gcgtgcgggc catctcagcc gaccggagct
    12361 cgtccatctc caggcgaagt atctgcgtga agccaaacgg tgagcttcga tccgttcggt
    12421 gatttcgcga ccgaggggta tctgcgaaat gtcgagaaag agaaggatct cgatatcgtc
    12481 aaaagactcg agcacacctc attcacgact gggttggacg aagccttcac cgcgctcgga
    12541 aaatccagac agttttccta ttcagacgtg ctgcgaacgc acggcatcct tttcaatgct
    12601 gtctatccat gggcgggaca ggatcgcctc tcgattgctc ctgcgctctc cgtccgcaag
    12661 ggacccgtca ttttcgccaa cccgtccgaa atacgaccag ccgttgagtt cgcgctgagg
    12721 aaggcgcagg aaaaagatta ccttcgcgca catcccggcg agatcatggg tcatctcgcc
    12781 tttggccatc cgtttctgga cggcaacggg cgcaccatcg tgaccctttt ctccgcgctg
    12841 gctcagcgcg ccggcttcag cgtcgattgg gctgcgaccg ataaggatgc ctatctggat
    12901 gcgctcacca aagaaattga cgtaccctca aagggccacc tcgataatta tctaggacaa
    12961 ttcattcgta agccgatcgc ccatgaggaa ctcgctgcac aaatcataaa cgcgcccggc
    13021 atcgatgggc gcgcgctcac gggcgacgaa aacgaagtca tcggaaacgt cgatgcgccc
    13081 gaggtgaagg ctcagtacga ggcgatgttg ctgaagcgcg ggaagaaaaa ttaggtaaat
    13141 caggggcctc gccaatagcc gatggcaact tccaacgcac agattgtcac aggaaatctc
    13201 gatactgccc aagaacatcg aaccggcgga ggaaatgtcg ctggtggcgc gcagtttggt
    13261 gcagagctac gggggatagc ggccgatctt gcccgaccgc gctgctcatc gcccgcgatc
    13321 tcccgcgctg gccaacgaga cgatcgggcg cgttgccgcc tttttgacgt tcttcgacca
    13381 taatcctgct ttggggtggg gttgggaacg ccggcgtttg tttgacatga cgagggcggt
    13441 tttgttgcgg cgaagggcga ctggggcatg caagtagcgt cggagcttat ctcgttgcag
    13501 aagcggtggc ggaatctgct cgtcgttggt gcaatttccg tgctgatcgg cgctggggca
    13561 catgctcaga atatcgatca gggcaaatcg gccgccaggc tgtttgcgga cggttgcgcc
    13621 acatgtcacc gcagcgcgcg cggtcttgca aagggccgct tccgccttac gctatacctg
    13681 ttcctgcagg accactacgc gagtaattca agctcggcct gggcgctcgc ctcttatctg
    13741 gagtccgtcg atagtccaca gcgcggccga tcgcgagccg ccgcggcgaa gccgtcgcgt
    13801 cctgccgtcg gcacatcacg gtcctcgatc ctccgtccgc ccgcgtcggt gccggggcgc
    13861 taggccggag aggcgcgcga ccgagcaccg cctcttggtt cggatggatg caggattgat
    13921 ttgagcctcg gtcgaaagca tccggcgact taagccgcaa tgttatccac atttccgact
    13981 acgttttgaa cgcatttgat ctgattcggc ggatgatccc gattcgcgag gatattgcta
    14041 tggcgttttt caagcgtgag ctcagcccgg cccagcgctt cgagcgtgcg ctgcgggaga
    14101 agcaggcggc gcggcagaag ctggccgacc agttgagcct cgccgaaacg gcacttggag
    14161 aaaaacgcga cgaagccgag cgcttggcgg tggccggcgc cgccaacgcc aagcttgctc
    14221 gcgcggaggc caggatgcgc accttcgagg accgcgccaa gaccctgcgc gccgcattgg
    14281 tcgaattcga cgagcaggtc gtcgcaaccg agcgcgcatt ggccgacgcc aaagcgcagc
    14341 gcgaccgcga cagggcggcc gacgagattg aggcgctggc tgctgcgatc gagcaggctg
    14401 ccccgggatt tggcgccggg gccgccgcac tcgtggaggc tgtcacaaaa agcatggcgt
    14461 cggtgccgga agcgaccatg ttctcgacta gcgtggatga gatgcggcgc gaggttctct
    14521 cggcagcgga cctgatctgt tgggagttgc gatccaccgc ggtgcgcacc cgcgcgggca
    14581 atgcgaatat cgcgctgctc gcgccacccg cgccaaagca actgcccgct ccggagatcg
    14641 aacggcaatt gatctacacg ctcagcccgc tgctttggcg ggagggcagc gaggtgcgca
    14701 gggttccggc gttcgcgctg gtcgggctgc caaaaatgtt actgctggtc gcgctgcggc
    14761 atcagcatgt ggactatctg aatgcccgac gcgtgcagac cctgatacac gttcacggca
    14821 gcggaggggt ccatagcgat cccgatcagg acgatccgca gctcgttgat ctcgatgcgc
    14881 tcgccgcaga ggagaaagag agcgcccagg ccgatgtggc gtgagcgcca aggaatcatg
    14941 atcggcaggc gattgcccac cacgagggcg gtcaggcggc ccactgatga gtgtgctgtg
    15001 atcaccatgt ccgttgatga aatcggacga ccagatattc gactttccga atatccggca
    15061 tttggccagt acgaacgatc agcgaagcga ggaaacgctc agacgtcgcg aggtccctgg
    15121 cgcggtgcga aggatgcaaa agaccctaga taggattttg gccgcgggag cgcgtaagca
    15181 ccaatttttg atgttttaag ccctagcgag ccgcaagctt ccgccagctt gaccgcgctc
    15241 gcgaccccgt cgatcacggg aagctcatgc ttttccgtca gacgtgccgc caattcagcc
    15301 atgccggcac atccgagaac gatcacctcg gcctggtcct cctgcttcgc gagttcgatt
    15361 tccgcactga tttgcgcggc tgcgtttgac gtgggatcat caagtgaaag aaccggaagt
    15421 tcgcatgctc gcaccttgcc gcagcgaccg gccagtccgt acttcaacag attgttttcg
    15481 atggcagcga tggagcgcga gagcgtcgtg acaaccgaga agcgatgacc cagcatcgag
    15541 gccagatgaa acgccgcctc gccgataccg atgacaggcg cgaatgagac gctacgcgcc
    15601 gcttcgaggc cggtgtcatc gaaacaggca atgatgtgtg cggaaacccc gctggcgtcg
    15661 cctttggcga tttcggccag tagtcccggc accgagaacg cctcgtcata atagccttcg
    15721 atcgagacgg gtccatccat cggattgacg gcgatgattt ccgtccccga ggccgccgcg
    15781 gcgcgggcgg ctcggccaat cttctcggtc atggcgacgg tggtattcgg attgacgaca
    15841 agaatgcgca tcaggccctt cctagaccat tcccttgccg gcatccgatc cgtgacgtcg
    15901 ttgtcgggcc tggagtacga gaatgacaat caacgcggag aaaatgacga gaaaggagac
    15961 ggcgctcgtc agtgttccga gcgcataaat gtccggcttt gtgacggttg tcgtcaggcc
    16021 ctgcagttca agtggcagag tgttgacgga accgatggcc tggcttgaac gcgcgagttc
    16081 gtcccacgac agggtaaaac cgaacagacc aatgcccacc acagatggca atatgatcgg
    16141 cagcacaacg tgccgaaacg tctgccaggg cgtggcgcca agatcacgcg ccgcctcttc
    16201 aagccggcca tcgaaccggt tgaagatcgc aaacatgatc aaaaggccaa agggcagggt
    16261 ccaggtgaga tgggcaccga ggcctgaggt gaaaagcccc atcgctgtcg tccaaccctc
    16321 cgagccgtgc tttgccatga aatcgtcgag cagccggaat tccagcgcga tgccgagcga
    16381 ggtgatgatc gacggcatga tcagactcgc gattgcggtg taaaacagga atgtcgcgcc
    16441 ccggaacgat ttgcggaagg ccaggccggc ggaaaccgac accacgaccg ttagcgccat
    16501 gacgacgagg ccgagcttca gcgaccgctt gaaggcagcc ccgatatcga cgatgccggt
    16561 gccggagaag accttgccga accactgcag ggacacacca ttcatcggaa aggtcagacc
    16621 gccatcaggt ccctgaaatg acagaatata gatcgcaaac atcggcccat acagaaacaa
    16681 cacataggcg atgaaaaacg cagcgaggaa ataaaaggag cgcggccggt tttcgcccac
    16741 ctcacagctc cttgcggata tcgacgatcc gcgtcagcga ggaaatgatc aaaatcgtga
    16801 taccaagcag gatgaccgcg ttggctgcgg cggccgggaa ttgcagtgca ttgaggcggg
    16861 cttcgatgat cttcccggcg gatgcgatct gttggccgcc cattacgccg atggtcacaa
    16921 aatcacccat cacgatcgtg atcacaaaga tcgagccgat gacgatgccc ggctttgcca
    16981 gtgggatgac gacgtttaca agggtctgcc aaccggtggc gcccgcatca taggcggcct
    17041 cgatcaacct cttgtcgatg cggatcatcg aattgaagat cggcaccacc atgaagaagg
    17101 tgaagaggtg aacgagcgcc agcacgacgg aaaattcgga gaacagcagc cattccagcg
    17161 gcgcccggat caatcccacg ccctcgaggc ccttgttgac cagaccgttc cggccaagaa
    17221 gcggaatcca cgcgatcatg cggatcacat tcgaggtcca gaatggaata gtgcaaagca
    17281 gggtgagtac gatctgccag gttttcgatc ggacgtggaa cgcgagaaaa taggcaacag
    17341 taaagccgat cagaagtgtc aggctccatg tcagcaggca caatttcaac gtcttcagat
    17401 aggtcttcag gatcgtgcac agatccggca gatcggcgat gcatccctcg aacgtatcgg
    17461 tgtagccgcg caggctgaag gcgggaatca tctcatattc attgtagtcc cacgcgctga
    17521 caatcgtcac aaaagccagc ggcaagagaa aaaacagcgc aaagatcagc atcatcggcg
    17581 cggcctgaag ccacgccacg aaggatttgc gttcttgaac catggaaagg ccgatctcgg
    17641 tcacctgaaa gggacttgcc gcgcggttta gcacggcaag tcctgagata gcatcaggcg
    17701 gcgatgaatt cattccactt gcggaccatg taatcgttct cgtccatgac ggcgttccag
    17761 caggcaacgg cgcccatgcg gtcgtcatag gacccgccgt cacgcttggc gccggctttc
    17821 tcgaggacgg tgccgtcggg cgccttgaca tcgccgacag ctgccttgcc ttccatccag
    17881 tagccccatt cgtcgggcgt catgttggcc ttggcggtgg agaggacggc ggaataatag
    17941 ccctggcgat tgagataagc accggcccag cctgacaaat accagttcac gaattcatag
    18001 gcccagtcga gtttcggtcc tgaaaccgcc ttcgacacgc agaaccccga cgcccacgag
    18061 cgatagcctt ctttgagcgg ctggaatgtg catgcaattc ccatggagcg cactttggtg
    18121 accgcaggcg accacatcga ctggatgacc gtttcgccgg atgccatcag gttgaccgat
    18181 tcgttgaaat ccttccagaa tgcgcggaac tggccggccc tcttggcttc ggtcatgatc
    18241 ttcatggtga gatcaatctc ggccttcgtc atgttgccct tgtcggcata tttgtgctgg
    18301 ccggtggctt ccaccaccat cgctgcatcc atgatgccga tcgacgggat gttgaggatc
    18361 gaggctttgc ccttgaattc gggattgagc aattcggtcc acgatgcaat cggccgcttg
    18421 atcaggtccg gccggatacc gagcgtatcg gcattgtaga ccgtcgggat cagcgtcacg
    18481 aattcggttg gcgtggtcga gaacttcttg gagttcggac cctcgagata aagcaccttc
    18541 cacggcgcgg tgccctgatc gccgatcttc ttgccgtttg gcaactgacc cttggtgaag
    18601 acgggggtga tgttatcaaa ctccttgatc ttcttggcgt cgagcgcgaa aatattgcca
    18661 gacggcacca gcttcttcag cgagaaatat tccgtatcga gcacatcaaa cgagttcggc
    18721 tgggtgatca cacgtttggt tacatcgtcg gtcgtggccg tgatgtattc aatcttgatc
    18781 ccggtatctt ccagacattt cttcgagata tcgtcgcctt cgttcaccgc tgtcccgaga
    18841 taacgcagaa ctttcggctc ggctgaatgg acatagggaa atccggtaat tgcgccggag
    18901 cctgctgcga ggccgaccgt tccggccgcg cccttcagca cagcgcgacg gctcacgcta
    18961 ctattgaatt tccaacgatt gctcatggtc atctcccaag gtgtcaagcc gccttttcag
    19021 gcgacgggac ggttacagaa ctgcgggtct tcagtgggct tgccagcgcc ggatcccagg
    19081 tggcgagaac acgttcgccg acggagtagt tggcggcgtc gaactggctt tccgtgagtt
    19141 gggccgagat gtctgcgccg ccgtcggcgg caatcgcgac gcggacatag gttccctggt
    19201 attcgacttc gctgacggtg ccagcgatcg acggaccatt gacggcttcg ctcggctttt
    19261 tcagcatcag acgatcattg cgaacggcga aggtctcgtc gtcgacggca acaacattgt
    19321 gaccgccgat gaatttcgcc gtgaattctg tccggggatg gttgtagatt tcgcgcggag
    19381 acccctgctg ttcgatccgc ccgccattca tcagaaccac gatatcagcc agcgccatcg
    19441 cttcatcctg gccgtgcgtg acatggatga aggagatccc aagctcgcgt tgaagttttt
    19501 tcaattcggc ccgcatgcgc agtcgcaaaa atggatcaag cgccgaaagc ggctcgtcca
    19561 gcagcaggat ctgaggagaa gtgatcagag cgcgggcaag cgccacgcgt tgctgctggc
    19621 cgccggaaag ctggccgggc agtctcgccg cataagcctg catgtcgaca agctggagca
    19681 atttttgggc ttcggcatgc cgctccgctt tggcaattcc cttcatcttc aaggcaaagg
    19741 caacattgtc gatcacggaa agatgcggaa acaaggcata g
//
LOCUS       DATNOI010000444        10337 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2443480,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000444 DATNOI010000000
VERSION     DATNOI010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10337
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2443480"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..172
                     /gene="rpmG"
                     /locus_tag="VN968_17800"
     CDS             5..172
                     /gene="rpmG"
                     /locus_tag="VN968_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HXL31123.1"
                     /translation="MAKAVTIKVKLVSSADTGFYYVAKKNSRTMTDKLVKKKYDPVAR
                     KHVEFKESKIK"
     gene            complement(249..1481)
                     /locus_tag="VN968_17805"
     CDS             complement(249..1481)
                     /locus_tag="VN968_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL31124.1"
                     /translation="MPLLQILRPTLPILIGASLMLSLSMGLRQSLGIFLQPLTHDVGI
                     SVSDFTLAIAVQNLAWGFLQPLAGALTVRFGFRTIMMAGAVFYIAGLSLMAGAQGFLS
                     IMLGAGVLIGMSLACTAAAIAMSVATRAVPATVRSTVLGMVSAAGSLGALLSAPMGQM
                     LNEGYGWRTGLAGFVILSLTLLPSAWFAGKVDKIPLPPRAPDQIADTSAAVATKIAFG
                     NASFVVMTCAYFVCGMQLVFITTHLPSYLAICGMDPMLSAQTLGVIGGFNVLGSLFFG
                     WAGERWNKLALLGGIYILRSLVLGWYFMLPPSPTTTLVFGAMMGFLWLGVGPLVAGAV
                     VEMFGLQWQAMIQGLAFMSHQLGSFVGAYGGGLIYDALGSYTMAWRIGVALGLAAGII
                     QVTFALLRPTEPPLVAAR"
     gene            1620..2273
                     /locus_tag="VN968_17810"
     CDS             1620..2273
                     /locus_tag="VN968_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL31125.1"
                     /translation="MSSPAKPTMKERILETADRLFYLQGIRAVGVDTVAAEIGISKRT
                     LYNHFPSKNELISAYLERRFRPVPTSDKPPAEQILRTFDRLERGFADKGFRGCPFVNA
                     VAEMGAEDHAVRKIAIAFKDSRRAWFRDLLLQLDVAHPDELATQFQLLVDGAIATDLV
                     RNDPSVARAAKDAARVLLRNAGVEVGPPDQAHKPERVTKKRSSSPRTRGPTRRDGAM"
     gene            complement(2356..3729)
                     /locus_tag="VN968_17815"
     CDS             complement(2356..3729)
                     /locus_tag="VN968_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HXL31126.1"
                     /translation="MSARILVVDDVPANVKLLEARLSAEYFDVLTASNGAEALEICAR
                     AECDIILLDVMMPDIDGFEVCRRLKSNPATHFIPVVMVTALDSPADRVRGLEAGADDF
                     LTKPVSDIVLIARVRSLTRLKMMTDELRMRAITSLEIGVQAPERSAVADTGKGGRILL
                     VDDRPSSYEKLAPVLSTEHSVDVEINPAEALFHAAEGNYDLLIVSLGLDNFDGLRLCS
                     QARSLERTRHVPILAIADADNNMRLLRGLEIGVNDYLLRPVDKNELLARARTQIRKRR
                     YTDHLRDNVQNSIEMAITDALTGLHNRRYMESHLGTLAEQAATRGKPLALMMLDIDFF
                     KSINDNYGHDAGDDVLREFAVRMRKSIRGIDLACRYGGEEFVIVMPETDLHVAGMVAE
                     RLRRSIAGEPFAVNKGTKRIEVTISIGLSTLELKGEPVADVLKRADTALYKAKNDGRN
                     RVVAAAA"
     gene            complement(3741..4106)
                     /locus_tag="VN968_17820"
     CDS             complement(3741..4106)
                     /locus_tag="VN968_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL31127.1"
                     /translation="MAKTVLIVEDNELNMKLFRDLLEAHGYQTSGTSNGFEALDLVRS
                     LRPDLILMDIQLPQVSGLEVTRWIKDDPELRSIPVVAVTAFAMKGDEERIREGGCEAY
                     LSKPISVGKFIETVRRFIG"
     gene            4330..4629
                     /locus_tag="VN968_17825"
     CDS             4330..4629
                     /locus_tag="VN968_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 domain-containing protein"
                     /protein_id="HXL31128.1"
                     /translation="MKKPVQNPREVAEIVAIQALSFVAGDPERLGLFLAESGIGPETL
                     RTAAADPRFLASILDFIMRDDATVKAFASASQLHPTNIAAARQALGDPDWERDVP"
     gene            4626..5918
                     /locus_tag="VN968_17830"
     CDS             4626..5918
                     /locus_tag="VN968_17830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HXL31129.1"
                     /translation="MTSAAPELGGPTCFCRDCLGDLDIAARRCGVCGSPRLVRHPALA
                     SLTLAHIDCDAFYATVEKRDKPEIADKPVIIGGGKRGVVSAACYVARTYGVRSAMPMF
                     KALALCPSAVVIRPDMAKYVRVGREVRHAMQALTPLVEPLSIDEAFLDLAGTQRVHGM
                     IPAKVLARFAQRVERDIGITVSVGLSCNKFLAKIASDMDKPRGFATLNQDEARAMLAE
                     KPVGFIFGVGPATQERLSQRGFRTIADLQRADEVDLMKQFSTEGRRLWRLARGIDDRR
                     VVPDRGAKTISNETTFETDIRDFATLEKTLWRLSEKVSSRLKSSDLSGLTITLKLKTA
                     DFRQRTRSQSIHAPTQLAAKIFAVSREMLAKEIDGTTFRLVGTGVSALRPGSLADDTD
                     MLDRRSAHAERAIDDLRKKFGNAAVIRGIAYDGPEKPG"
     gene            6243..7469
                     /locus_tag="VN968_17835"
     CDS             6243..7469
                     /locus_tag="VN968_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HXL31130.1"
                     /translation="MCTLPHHAGRSRDGADGTNRRDMLKRLAAMGLTAPLVTLILDRV
                     AMAGSISFLDRAELIEGVTALIARAKSPELREARVFELSGGDLPWKDLGITLAKGQEA
                     TFLLGGRVWLSREQDLWVEPGVAFHVRTRGTRPIFNPMSNTGTMTAADNGPLEMARSL
                     VEFQNEEGELAVPKNEYVKADARIYGLALVWRGSALAGLESLLRHGDVDGIVAAELAR
                     LQSPRKLPAGWNNFFMLGGGPVIFNDIGNGAISCEPLKNAGILQRPVGIELKPQTKLS
                     WRWIVEELPSRLPEDQLTSHDYLSIAAEFDDGQDLTYFWSSSLPVGKAFRCPIPRWTP
                     LESHMVVRSGLADLGKWVSDERDIYADYKEHIGGPAKSVVRVWLLGVSLFQRRAGSCR
                     FGDMQISQPGAGTLKL"
     gene            7794..8249
                     /locus_tag="VN968_17840"
     CDS             7794..8249
                     /locus_tag="VN968_17840"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXL31131.1"
                     /translation="MSYVERVLQPGEQIRHISSIHWIVYWAGVGVALLAAVAYWLSET
                     RPLTRFWLYMACALALIAVALLVQEWYRWWITEIAVTNRRVIYKKGFIRRRTNEMNMH
                     KIESVQVYQSILGRMLGYGTVTILGAGAGFETLRTIAGPIELRNSITGT"
     gene            complement(8657..9571)
                     /locus_tag="VN968_17845"
     CDS             complement(8657..9571)
                     /locus_tag="VN968_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXL31132.1"
                     /translation="MRTATAADGPPAWAYPPNNPDYKPPVDEGKLVRVPDSMAGYTWS
                     QLRDRFIAPVWHPGDHAALPDIVANGRKPGVFACGFCHRAAGPGGPENADLAGLPKSY
                     IIGQMADYKSGARGTTVPKRTPPDLMISLSKLITDAEVEAAAAYFSALQPRKRIKVVE
                     SDTAPKTYVAGLIWAAVETGEREPVGARVVEVPDDLLGFESRDSRSTFTAYVPIGSLA
                     KGEALVMKGGPGKTIQCALCHGTDLKGLGPMPSIAGRSPSYMFRQLYDLQHGARTGEW
                     SPLMAQVVANLDEQDLLAIVAYLASRDP"
     gene            complement(10061..>10337)
                     /locus_tag="VN968_17850"
     CDS             complement(10061..>10337)
                     /locus_tag="VN968_17850"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL31133.1"
                     /translation="YDQAIAINPKDFNAFNSRGKAYVAKGDSNQAIADFTQAIQFGPS
                     YVEAYNNRGLSNEVLGHRAEAIADFRKAQSIDPADRVSKQQLRVFGF"
BASE COUNT         2053 a         3242 c         3164 g         1878 t
ORIGIN      
        1 gaacatggcc aaagcggtca ccatcaaggt caagctcgtt tcctcggcgg ataccggctt
       61 ctattacgtc gccaagaaga attcgcgcac catgaccgac aagctggtga agaagaagta
      121 cgacccggtc gcgcgcaagc acgtcgaatt caaagaatcc aagatcaagt aaggttttgg
      181 cacatcgctg gcggatttga acggggcctt gcggccccgt tttgatttgg agccgtgagc
      241 gaagtgcttt atcgcgcggc caccaacggc ggctcggtcg gccgcagcaa tgcaaatgtc
      301 acctggatga tccccgccgc aagacccagg gcgacgccga tccgccaggc catcgtgtag
      361 gaaccgagcg cgtcgtagat cagcccgccg ccataggcgc caacgaagct gccgagctga
      421 tggctcatga aggcaagccc ctggatcatc gcctgccatt gcagcccgaa catctcgacg
      481 accgcgcccg ccaccagcgg cccgacgccg agccacagga aacccatcat cgcgccgaac
      541 accagtgtcg ttgtgggtga tggcggcagc atgaagtacc agcccagcac cagcgacctc
      601 aaaatgtaga ttccgcccag cagcgccagc ttgttccagc gctcgccggc ccagccgaag
      661 aacaggctgc ccaggacgtt gaaaccgccg atgacgccga gcgtctgcgc actcagcatc
      721 ggatccatgc cgcagatcgc cagataagac ggcaggtgcg tggtgatgaa gacgagctgc
      781 atgccacaga cgaagtaggc gcacgtcatc accacgaaag aggcgttgcc gaacgcgatc
      841 ttcgtcgcga cggcggccga agtatcggca atttggtcgg gcgcccgcgg cggcagcgga
      901 atcttgtcga ccttgccggc gaaccaggcg gatggaagca gcgtcagcga caggatgaca
      961 aaaccggcga ggccggttct ccagccgtac ccctcattca gcatctgccc catgggcgcc
     1021 gacaatagcg ccccgagcga tcccgccgcc gacaccatgc ccagcaccgt gctgcgcacg
     1081 gtcgcgggga ccgcgcgcgt tgccaccgac atcgcgatcg cagccgccgt gcaggccagg
     1141 gacatcccga tcagcacccc cgcgccgagc atgatgctga gaaaaccttg cgcgcctgcc
     1201 attagcgaga gcccggcgat gtaaaagacc gcaccggcca tcatgatggt gcgaaagccg
     1261 aaccgcaccg tcaacgcgcc ggcgagaggt tgcaggaaac cccaggcgag gttttgtacg
     1321 gcgatggcta gcgtgaaatc ggaaaccgat ataccgacat catgggtcag cggctgcagg
     1381 aaaatgccaa gtgactggcg caaccccatg ctcaggctga gcatcagcga ggcgccgatc
     1441 agaatcggaa gtgtgggacg aaggatctgc agcaggggca tattctttgt tctccctttg
     1501 gcccgccggt cggcggaccg cgactgccat gacgatcagc gcttgattac accgatctgt
     1561 gtaactaagt tatgaggcgt tgcattgtca aggcaaaaag aacacaggtc tgtgtaatca
     1621 tgtcctcccc cgcaaagccg accatgaagg agcggatcct cgaaaccgcg gaccggctgt
     1681 tctatctgca gggcatccgc gccgttggcg tcgataccgt cgcggccgaa atcggcatct
     1741 ccaagcgcac gctctacaac cactttccct cgaaaaacga gttgatatca gcctatctcg
     1801 agcgccggtt caggccggtt ccgacttccg acaagccgcc cgcagaacaa atcctgcgca
     1861 ccttcgaccg gctggagcgc ggcttcgccg acaagggctt tcgcggctgc ccgttcgtca
     1921 acgcggttgc cgaaatgggc gctgaggatc atgcggtcag aaagatcgcg atcgccttca
     1981 aggacagccg ccgcgcctgg ttccgcgacc tgttgctgca gctcgatgtc gcccatccgg
     2041 atgagcttgc gacgcagttc cagcttctcg tcgatggcgc gatcgccacc gatctggtgc
     2101 ggaacgatcc gtcggtggcg cgcgccgcaa aagacgccgc gcgcgtgttg ctcaggaatg
     2161 cgggagttga ggttggcccg cccgatcagg cacacaaacc tgaacgcgtc acgaaaaaac
     2221 gatcgtcgtc cccgcgaacg cggggaccca cacgccgtga cggtgcaatg taaaacaggc
     2281 ggctggggca agaattcgct tcaaccataa ccgacggtgg gggtcgccgc gttccgccgg
     2341 gacgacgtgg ccggactacg ccgccgccgc cacgacgcgg ttgcggccgt cgttttttgc
     2401 tttgtagagc gcggtgtcgg cgcgcttgag gacgtcggcg acgggctcgc ccttgagttc
     2461 gagggtcgac agcccgatcg agatcgtgac ctcgatgcgt tttgtacctt tgttgaccgc
     2521 aaagggctca cccgcgatcg aacgccgcag ccgttcggcc accatgcccg cgacatgcag
     2581 atcggtttcc ggcatgacga tgacgaattc ctcgccgccg tagcggcagg cgagatcgat
     2641 gccccggatc gacttgcgca tccggacagc gaattcgcgc aatacgtcgt cgccggcgtc
     2701 gtggccgtaa ttgtcgttga tcgatttgaa aaaatcgatg tcgagcatca tcagcgccag
     2761 gggcttgccg cgggtcgcgg cctgctcggc cagcgttccc agatggcttt ccatgtaacg
     2821 gcggttatgc aggccggtca gggcgtccgt gatcgccatc tcgatcgaat tttgcacatt
     2881 gtcgcgcaga tggtcggtgt agcgccgctt gcggatctgg gtgcgcgcac gcgccagcag
     2941 ttcgttcttg tcgaccgggc gcagcaggta atcattgacg ccgatctcca ggccgcggag
     3001 cagccgcata ttgttgtcgg cgtcggcgat ggcgagaatg ggcacgtgcc gggttcgctc
     3061 cagcgaccgc gcctggctgc acagccgcaa gccatcgaaa ttatcaaggc cgagcgagac
     3121 gatcaaaaga tcgtaattgc cttcggcggc atgaaacagc gcttccgccg gattgatttc
     3181 gacgtcgacg gaatgctcgg tgctcagcac cggcgccagc ttctcgtagg atgacggccg
     3241 gtcgtcgacc agcaggatgc gtccgccttt cccggtgtcg gccaccgcac ttcgctcggg
     3301 cgcctgcacg ccgatctcaa gcgaggtgat ggcgcgcatg cgcagttcat cggtcatcat
     3361 cttcagccgg gtcagcgaac gcacccgtgc aatcagcacg atgtccgata ccggcttggt
     3421 caggaaatcg tcggcgccgg cctcaagacc gcgcacgcga tcggccgggc tatccagtgc
     3481 cgtcaccatc accaccggaa tgaaatgcgt cgccggattg gactttagcc ggcggcaaac
     3541 ctcgaaacca tcgatatcgg gcatcatcac gtcgagcagg atgatgtcgc attcggcgcg
     3601 cgcacagatc tcgagcgcct cggcgccgtt ggacgcggtc aggacgtcga aatattcggc
     3661 cgacagccgc gcttccaaaa gcttgacgtt ggcggggacg tcgtcgacga ccagaatgcg
     3721 cgcggacatc gaagctctcc ctagccgata aaccgtcgga cggtttcaat aaatttgccg
     3781 accgagatcg gttttgaaag ataggcctcg cagccgccct cgcggatgcg ttcctcgtcg
     3841 cccttcatcg cgaaggccgt gaccgcaacc accggaatcg agcgcaactc gggatcatcc
     3901 ttgatccatc gtgttacctc cagtccggaa acttgcggca gctgaatatc catgaggatg
     3961 agatcgggac gaagcgagcg gacgagatca agcgcctcga aaccgttgct ggtgccggag
     4021 gtctgatagc catgcgcttc caacagatcg cgaaagagct tcatattgag ctcgttgtcc
     4081 tccacgatca ggacggtttt tgccatccgt ccctccaatc cccaggcgac aggccggcat
     4141 gcggtgcatc aggcgacgcc gcgggcgtgt cgaaaagtga attcaaacta gcgtcagatt
     4201 cgctcaaagc agttaagtcc gggccatctt taagcgaagt ggtttccact tgcttgaaca
     4261 cgttccgcag actcgggcat gatggtttca aaatagagac cataagttac ggaaaggcaa
     4321 acacatcagt tgaaaaaacc ggtccaaaat ccccgggaag tggctgaaat cgttgcgatt
     4381 caggcacttt ccttcgtcgc cggcgatccc gaacggctgg gcctgttcct ggccgaaagc
     4441 ggaatcggcc ccgagacgtt gcgaacggcc gccgccgatc cgcggtttct cgcctcgata
     4501 ctcgatttca tcatgcggga tgatgcaacc gtgaaggcgt tcgcgagcgc ctcgcagctg
     4561 catcccacca acattgccgc ggcccgccag gccctgggcg accccgattg ggagcgcgat
     4621 gtgccgtgac gtcagccgcg cctgagcttg gcggcccaac ctgcttttgc cgggattgcc
     4681 tcggcgatct cgacatcgcg gccaggcgat gcggcgtatg cggctcgcca cggctggtcc
     4741 gtcacccggc gctggcttcg ctcaccctcg cccacatcga ttgcgatgcg ttctacgcga
     4801 cggtggagaa acgcgacaaa cctgaaatcg ccgacaagcc tgtgatcatc ggcggcggca
     4861 agcgcggcgt ggtctccgcg gcctgctacg tcgcccggac ctatggcgtg cgctcggcga
     4921 tgccgatgtt caaggcgctg gcgttgtgcc cgtccgcggt cgtgatccgg ccggacatgg
     4981 caaagtatgt cagggtcggc cgcgaggtgc gccacgccat gcaggcgctg acgccgctgg
     5041 tcgagccgct ctcgatcgac gaggcgtttc tggatcttgc cggcacccag cgcgttcacg
     5101 gcatgatccc cgcaaaagtg ctggcgcgct tcgcccaacg ggtcgaacgc gacatcggca
     5161 tcacggtatc cgtcggtctc tcctgcaaca aattcctggc caagatcgca tccgacatgg
     5221 acaagccgcg cggttttgca acgcttaacc aggacgaagc gcgcgccatg ctggccgaaa
     5281 aaccggtcgg attcatcttc ggcgtgggac ccgcgaccca ggagcggctg tcgcagcgcg
     5341 gctttcgcac catcgccgat ctgcagcgcg ccgatgaagt cgacctgatg aagcaattct
     5401 cgaccgaggg ccgccggttg tggcggctgg cgcgcggcat cgacgatcgt cgcgtggtgc
     5461 ccgatcgcgg cgccaagacc atttccaacg agaccacgtt cgagaccgac atccgcgatt
     5521 ttgccaccct cgagaagacg ctgtggcggc tgtcggaaaa ggtatcgtcg cgcctcaaga
     5581 gcagcgatct ctccggcctg accatcacgc tgaaattgaa gacggccgat ttcaggcagc
     5641 gcacccgctc gcaatcgatt cacgccccga cccagctggc ggcgaaaatt ttcgcggttt
     5701 cccgcgagat gctggcgaag gaaattgacg gcaccacgtt ccggctggtg gggaccggcg
     5761 tcagcgcgct gcgcccggga tcactggccg acgacaccga catgctcgac cgccgctcgg
     5821 cccatgccga acgcgcgatc gatgaccttc gcaagaagtt tggtaacgcc gccgtgatcc
     5881 gaggcattgc ctatgacggg ccggagaagc cggggtaacg ggaaacccaa ctaccgttat
     5941 tgccgggctt gacccggcaa tccatccgtt tccagaaatt cttgcagaga gatggatgcg
     6001 cgggtcaagc ccgctcatga cggtaagagc gtgcagccac gtttacttac acggcttgga
     6061 atccttgagc gactcgctgt tgggcgggca acggtccacg ccggcccgct ccttgacctc
     6121 gggcaatttt cccgggcgat gccgggaaac cgtctcaagc cttcctccgc cctctgcgct
     6181 atggcctcaa gtccagggct tcgctccgca acggcgcgaa tgaacaatca gggagggaca
     6241 tcatgtgcac attgcctcac catgccggcc gctctcggga cggtgcagac gggaccaatc
     6301 ggcgcgacat gctgaaaagg ctcgcagcca tgggactgac ggcgccgctc gtcacgctca
     6361 tcctcgatcg cgtggccatg gccggatcga tcagcttcct cgaccgtgcg gagctcattg
     6421 agggcgtgac ggcgctcatc gccagagcaa aatcccccga gctccgcgag gcccgcgtct
     6481 tcgagctcag cggcggcgac ttgccgtgga aggacctggg gatcaccctc gccaaagggc
     6541 aggaagcgac attcctgctc ggcggccgcg tgtggctctc ccgtgagcag gatctctggg
     6601 tcgagccagg cgttgcgttt cacgtgcgca cccgaggcac ccgtcccata ttcaatccga
     6661 tgagcaacac cgggacgatg acggccgccg acaacggtcc cctcgagatg gcgcgatcgc
     6721 tcgtcgagtt tcagaacgag gaaggcgagc tggcggtgcc gaagaatgag tatgtcaagg
     6781 cggacgcacg catctacggc ctggcgctcg tctggcgagg cagcgcgctc gcgggattgg
     6841 aaagcctgct caggcacggc gacgtcgacg gcatcgtcgc cgcggaattg gcgcgccttc
     6901 agtcgccccg caagttgccg gcaggctgga ataatttctt catgctgggt ggtggcccgg
     6961 tcattttcaa cgatatcggc aatggcgcaa tttcctgcga gccgttgaaa aacgcaggca
     7021 ttctgcagcg ccccgtcggc atcgaattga agccgcagac gaagttgagc tggcgctgga
     7081 ttgtcgagga gctgccgtcg cggctgccag aagaccagct cacgtcgcac gactatctct
     7141 caatcgctgc ggagttcgac gatggccagg acctcaccta cttctggagc agcagcctcc
     7201 ctgtcggcaa ggcgttccgt tgcccgatcc cccggtggac gccgctcgaa tcgcacatgg
     7261 tggtccgcag cggtcttgcc gatctcggca agtgggtcag cgacgagcgt gacatctatg
     7321 cggactacaa ggagcacatc ggcggtccag ctaagtccgt cgtacgcgtc tggctgctcg
     7381 gcgtctccct cttccagcgc cgcgccggtt cgtgccgctt cggcgacatg cagatctccc
     7441 agccgggcgc cggaaccctg aagctttgag acaccgcgag agtgaaccgg ttcaatcggc
     7501 tcgctgctag cagttccgag ggcgtggaaa aacgcttacg ttcttctgct atgggtggtc
     7561 aaaaacggaa gtgtccgcgc cgcgcggcac gtccgtttca ccctcaacag cggactcttg
     7621 caccgcagca atcagattcg atcacctcct cggcgcaagc gagaagcgtt gccggcactt
     7681 cgaggggccc agcacctacg ccgcatcgat ccgtcggcga gttcaaccgc tgcgccggag
     7741 gttggcacag ctccgggggt tcgctatcct gcgcgggtag gagacccgtt gttatgagct
     7801 acgtggagcg cgtcctgcag cctggtgagc aaatccggca catctcgtcg attcattgga
     7861 tcgtgtactg ggccggcgtt ggggttgcgc ttctggcggc ggttgcttac tggctcagcg
     7921 aaacgcggcc cctgacgaga ttctggctat atatggcttg tgcgctcgcg ctcattgcgg
     7981 tcgctctcct ggtccaagaa tggtacagat ggtggatcac cgagattgcg gtcaccaacc
     8041 ggcgcgtcat ctacaagaaa ggtttcatcc ggcggcggac caacgagatg aacatgcaca
     8101 agatcgagag cgtgcaggtc tatcagtcga tcctcgggcg catgctcggc tacggcaccg
     8161 tgaccatcct tggggccggc gcaggcttcg agacgctacg gacgatcgca ggcccgattg
     8221 agctgcgaaa ctccatcacc ggcacctgac tgggccgaat cgcaagggca tcgcgtttgg
     8281 cgaaaatgct tcaaggcaag acttctgcgc gattgttgag ggtcaaacta agaagaactc
     8341 agtgtgagca aatgtcttcc gagttaccct gaaagcggac atcgctcaat tcagtcggca
     8401 tgtctcaaag gtgccggaac cggactcatc caacacattc catgaagaga ggttgcgatt
     8461 gcgcggcgtg acccatgacc ttgccagtcg gcggattcac caaattggtg ttgaggcgcg
     8521 ccgtgcaggt cgaagaagtg agtgacactg aaacaggact tgtagtgatg aggtcgccaa
     8581 ctagcgcggc ctcgcacgcg ggagcgcgca tgaccacatc ccggtgatgg cctggaggcg
     8641 cacattcgta cccgtttcac ggatcgcgag atgcgaggta cgctacaatc gcaagcaggt
     8701 cctgctcgtc gaggttggcc acgacttggg ccatcagcgg gctccattct cccgtccgcg
     8761 cgccatgctg gaggtcatac agctgccgga acatgtagct cggcgaacga ccggcaatgc
     8821 tcggcatcgg tcccagccct ttcaggtcag tgccgtggca caacgcgcat tggatcgttt
     8881 tgcccggccc acctttcatg accagcgctt cgcctttggc aaggctcccg atcgggacat
     8941 aggctgtgaa ggtcgaccgc gagtcgcggc tttcgaagcc gagcaaatcg tctgggactt
     9001 ccaccacccg cgctcctacc ggctcgcgct cgcccgtttc cacggcggcc caaatcagac
     9061 ccgcaacata ggtcttcggc gcggtatcgc tctcgaccac cttgatgcgc ttgcggggct
     9121 gcaaggccga gaagtaggca gctgcagctt cgacttcggc gtcagtgatc agcttggaga
     9181 ggctgatcat cagatccggc ggcgttcgct tcgggacggt cgtaccacgc gcgccgctct
     9241 tgtagtctgc catctgcccg atgatgtagc tcttcggcag ccctgcgaga tccgcgtttt
     9301 ccgggccgcc gggaccggcg gcgcggtgac aaaatccgca agcgaacaca ccaggtttcc
     9361 ggccgttcgc aacgatgtcc ggcagcgccg catggtcgcc ggggtgccag accggcgcaa
     9421 tgaaacgatc gcgaagttgg ctccaggtgt acccggccat gctgtctggc acccgaacca
     9481 gctttccctc gtcgacggga ggcttgtagt ctgggttgtt tggcggatag gcccaagcag
     9541 gaggaccgtc tgcagcggtt gccgtccgca tcgcgagcaa gccgcaaacg atcacagacg
     9601 agagcagcgc gacaacaatc ttcatttcca cctcccgaat gcccgagctc aaagctcatc
     9661 gagactccca acagggagca cccatccgag tgagaaccga tttcaaaaat tgaaatcgcg
     9721 cgaaccgatg gcaccaaggc tgccaaatgg atgaaacggc gccttcggta cctcgccgtt
     9781 tttgggcagc tctcgatcaa gcaaatctgc acaaccgtca gtggaacgct acagcatgtc
     9841 aggccccacg cctagtgtca gctccgggtc aaaagcgcca ttctgactga gcgccaatca
     9901 cttcccgtct tccccgatga gggtgagcaa atctcgcccg agattcgctc aagagcagac
     9961 atcgagacgg ccagtagccg atcggtgtcg caaaagcgac gcagcacgga cgagtcggat
    10021 cgcgggtccg ccccttgagc agaatttgaa cggcacgccg tcagaaaccg aacacgcgga
    10081 gctgttgctt gctgacccgg tcggccgggt cgatcgattg cgccttacgg aaatcggcga
    10141 tcgcctcggc gcggtggccc agcacttcat tggagaggcc gcggttgttg taggcctcga
    10201 cataggacgg accgaactgg atcgcttgcg tgaaatctgc aatcgcttgg ttgctgtcac
    10261 cctttgcgac gtaggctttg ccgcgggagt tgaaagcgtt gaagtccttg gggtttatcg
    10321 cgatcgcctg gtcatag
//
LOCUS       DATNOI010000445        10085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_202465,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000445 DATNOI010000000
VERSION     DATNOI010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202465"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VN968_17855"
     CDS             complement(<1..600)
                     /locus_tag="VN968_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31134.1"
                     /translation="MLAGAAQAAPGDKKDVVRDLAGRVGPMVGSALACRDIAPPRIQV
                     IVDKFTAVITQFSASEAERADLTQLLDRNVADGRAAVTSGRMDCRLAERQLADLEQSI
                     TRPPLPAAAPPSATVTPSFAAIGATAPTTSVTQTAGGPPVRGITDQEIRFGMAAPFSG
                     AAKELGRQMKLGIDTAFNRINEAGGVDGRMLKLVTADDGY"
     gene            complement(752..2317)
                     /locus_tag="VN968_17860"
     CDS             complement(752..2317)
                     /locus_tag="VN968_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31135.1"
                     /translation="MSLRWMMVVSGALLAGTGSVSAGDKTYIVRDLAGRVGPIIGSAL
                     ACRDIARPRIQVIVDKFTAVITENSSNEAERSDLTLLLDRNVADGRSAVTTGRIDCVA
                     ADRQLADLERSIAPRSASPAPPASSIISPSSAVAATAPTAPVSVAPPVRGITDREIKF
                     GIAAAFTGPTKELGRNMKLGIDTAFNRTNDAGGVEGRMLKLVSSDDGNDPTRTLNAMT
                     QLYDKEQIFGIIGNTGTPTGAVAVPYALERRMLFFGAFTGANVVRHDPPDRYVFNYRP
                     SYAEETDAAVRYLVKLRRIPVRQIAVFAQQDGFGDSGFAGVAKAFRALGVDDSGILRL
                     NYQRNTVDVDDAINQLKAQRVPIKAVVMVATYRVAAKFIEKTHDLFPGMIYTDVSAVG
                     GTALADELMLLGPRYATGAIVTQVVPAVSGYSSIVLEYKTALAKYFPGESPDYVSLEG
                     FISANILIQALKRCGPQLDTEKLIDVLESMRNIDLGLGVPLNFGRAEHQASHKIWGTA
                     LDDSGKFQAIELE"
     gene            complement(2448..4010)
                     /locus_tag="VN968_17865"
     CDS             complement(2448..4010)
                     /locus_tag="VN968_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31136.1"
                     /translation="MTLRWMTVVFGALLAGAGGAAAGDKTDVVRDLAGRVGPIVGSAL
                     ACRDISRPRIQVIVDKFTAVISEASSGEAERSDLTQLLDRSVADGRSAVTSGRIDCRV
                     ADRQLGDLERSIAGPGPSLAAVIAPSFATAAATAPTAPVAAIAGPPVRGVTDREIRFG
                     IAGPFSGSSKELGRQMKLGIDAAFNRTNDAGGVEGRMLKLIAADDGFEPTRTAEAMKQ
                     LYEKDQVFGLIGNVGTATAAVAVPYALERRMLFFGGFTGANVIRHDPPDRYVFNYRAS
                     YAEETDAAVRYFVKMRRIPPRQIAVFAQHDAGGDAGFAGVAKSFRSLGFSDSGILRLN
                     YERNTVEVEDAVNQLKAQKVPIKAVVMISAYRPAARFIEKTRDLFPGMLYSNISFVGA
                     TALADELMLLGPRYASGAIVTQVVPSVSGYSSIVLEYKTAIAKYFPGEAPDYVSLEGY
                     VSANILIQALKRCGPQLDTEKLIDVLENTRQLDLGLGAQLGFGRAEHQASHKIWGTAL
                     DESGKYQAIELE"
     gene            complement(4108..4731)
                     /locus_tag="VN968_17870"
     CDS             complement(4108..4731)
                     /locus_tag="VN968_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31137.1"
                     /translation="MSSLSSEQDHLNPNDQLYYAPRWLRERSEPVSASPSPVPAPAAE
                     KKPESLARPISPPHSFDALLEEAVAESLRHPLDPQVMNEPPGFVRELDRRMAILSVAG
                     RFAAAIGVSAIVALFFVIMVPASRDYSRQQPDADNSSVSGFLQSLKTALSQPRQKDDD
                     AKPALAEFQAILASPPTTPPPVSREESETLLQQFVQWQQKPAQPTAP"
     gene            5411..6103
                     /gene="minC"
                     /locus_tag="VN968_17875"
     CDS             5411..6103
                     /gene="minC"
                     /locus_tag="VN968_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HXL31138.1"
                     /translation="MVARAEPVRQQMRLRGRSYVAFVFCPFVPIVGWLEEIDATLARS
                     PGFFVGKPVVLDLSAVDLSQSAIAHLVTSLESRSIRVLGIEGVAAAHVTTSMPPLLTG
                     GRHCVVPQNKPEKPAARPKPTSLLLESPVRSGQSVIFAEGDVTVLGSVSSGAEIVAGG
                     SIHIYGTLRGRAMAGVNGNPAARIYCQKIEAELLAIDGYYQTAEQIDASVRNGPAQAW
                     LEGDVMKITPLN"
     gene            6149..6964
                     /gene="minD"
                     /locus_tag="VN968_17880"
     CDS             6149..6964
                     /gene="minD"
                     /locus_tag="VN968_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HXL31139.1"
                     /translation="MAKVLVVTSGKGGVGKTTSTAALGAALAQSGEKVVVVDFDVGLR
                     NLDLVMGAERRVVFDLINVVQGVAKLSQALIRDKRLENLWLLPASQTRDKEALTDEGV
                     GSIIAELRTKFDWILCDSPAGIERGAMLAMRYADEAVIITNPEVSSVRDSDRIIGMLD
                     SKTVKAEKGELVKKHVLITRYDTARAARGEMLTIEDILEILATPLLGIIPESQDVLRA
                     SNVGSPVTLSNAASAPARAYTDAARRLMGETVTMLVPAERKGFMDRLLGRRAA"
     gene            6961..7254
                     /gene="minE"
                     /locus_tag="VN968_17885"
     CDS             6961..7254
                     /gene="minE"
                     /locus_tag="VN968_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HXL31140.1"
                     /translation="MSASLLRFFSGRKSAPASASAPIARERLQILLAHERSMRGEPDL
                     LVLLRDEILAVVSRHVTLDPDRVIVKMDRGDTVSTLEVDIELPNGFERIAAAG"
     gene            7362..7850
                     /locus_tag="VN968_17890"
     CDS             7362..7850
                     /locus_tag="VN968_17890"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXL31141.1"
                     /translation="MSPKRPALPMTGGCPCGVIRYQITSFPLLLYTCNCTDCQRASGS
                     AFALNMPVRARDFHILQGEPKGWRHTSPTGVAVISWFCGDCGGRLYGDRAAREAIVNL
                     RAGTLDDTTWLVPIAHMYTRSAQPWVAPAVNAECYEIGPDDFRPLAERWRAMWPEFLL
                     EK"
     gene            7909..9408
                     /locus_tag="VN968_17895"
     CDS             7909..9408
                     /locus_tag="VN968_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018115205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HXL31142.1"
                     /translation="MNLVEPTTSKAKTGIWGLDDILSGGFSRGHMFLVEGAPGTGKTT
                     IALQFLMEGAQAGEKCLYITLSETERELREGAASHGWALEDGIEVFELLPPESLLDSE
                     QQQSLLYSSDLELGETTKQIFGAVERARPSRVVLDSLSEIRLLAQSSLRYRRQILAIK
                     HYFAKFGTTVMLLDDLTAELADKTVHSVAHGVLRLEELAPAYGAERRRARIVKYRGVM
                     FRGGYHDATITTGGLNIFPRLVASEYRSSFVRTRMSSGIAELDRLLGGGIEAGSSTLV
                     LGPAGTGKSLLAIVFIAAAIARGEKAALFVFDEELGLLFARMKGLGIDLEAMQRSGNL
                     FVEQVDAAELLPGEFAHRVRKRVDEDDIKTVVIDSLNGYQAAMPEENSLILHMHELLQ
                     YLNRRGAATFMTVAQHGLVGDMKAPVDVTYLADTVMLLRYFEALGTVRRAISIIKKRT
                     GAHESTIREYRIDGRGLTIGDPLDGFQGVLRGVPVYVGEGRPLLEEQVQ"
     gene            9405..>10085
                     /locus_tag="VN968_17900"
     CDS             9405..>10085
                     /locus_tag="VN968_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXL31143.1"
                     /translation="MKPGASSERAVVLAPTGRDATVATALIREAGYHANVCGDLAALL
                     HEIEGGAGLAVIADEAIKTADLRGLMRWLNDQPSWSDFPIVLLTHQGGGPERNPDAMR
                     LGQVLGNVTFIERPFHPTTLVSVVGSAVRGRRRQYQTRAILQDLTESEGLLQTALSAG
                     RLGALELHLPELELEASDTCKTFFGRKPGEPFSYQDLLAAVHPDDRARRLEVLDRAIE
                     TGRDYSIEY"
BASE COUNT         1915 a         3004 c         3194 g         1972 t
ORIGIN      
        1 ataaccgtca tcggccgtga ccagcttgag catccggccg tccacgccgc cagcctcgtt
       61 gatccggttg aaggcagtat cgatcccgag cttcatctgg cgcccaagtt ccttggccgc
      121 gccggaaaac ggcgccgcca ttccgaaccg gatttcctga tcggttatgc cgcggaccgg
      181 ggggccgccg gcggtctgtg tgaccgatgt ggttggcgcg gtggctccaa tggccgcgaa
      241 ggaaggtgtg acggtggcgg acggtggcgc ggcggcgggt aaaggcggcc gggtgatcga
      301 ctgctcgaga tcagccagtt ggcgttccgc cagtctgcaa tccatccgcc ccgacgtcac
      361 ggcggcacga ccgtcggcga cattgcgatc gagcagttgg gtcaggtcag cgcgctcggc
      421 ctcgcttgcg gagaattggg tgatgactgc cgtgaacttg tcgacgatga cctggatacg
      481 tgggggtgca atgtcacggc aggccaacgc cgagccgacc atcggcccca ctcggcccgc
      541 aagatcgcgt acgacatctt tcttgtcgcc cggcgcggct tgcgcagcgc ctgcgagcaa
      601 ggcgctaact aataggatcg tccaacgaag gtccaccgca taattccgtg agtttaagga
      661 gtcgaggatt tctatgagag aacatgatct gcacggcgtt gaccctttct aggcgattgc
      721 accaatatta ttctattgtt ctatggttct atcattctag ttcgatggcc tgaaacttgc
      781 cgctgtcatc cagcgccgtt ccccagatct tgtgcgaagc ctggtgttcg gcgcggccaa
      841 agttaagtgg cactccgagg cccagatcga tattgcgcat gctctccagg acgtcgatga
      901 gcttttcggt atcgagctgc gggccgcaac gcttaagtgc ctggatcaga atattggccg
      961 aaatgaagcc ttccagcgaa acgtagtcgg gcgactcgcc gggaaaatac ttggccaagg
     1021 ccgtcttgta ttcgagaacg atgctcgaat aaccggatac cgccggcacc acttgggtca
     1081 cgatcgcgcc ggtcgcgtag cgcggcccca acagcatcaa ttcatcggcg agcgcggtgc
     1141 cgccgaccgc cgacacgtcg gtgtagatca ttccgggaaa caggtcgtgc gtcttctcga
     1201 tgaatttggc ggcgacccga taggttgcaa ccatgaccac tgccttgatg ggcactcttt
     1261 gcgccttcaa ttgattgatc gcgtcgtcga cgtccaccgt gttgcgctga tagttgagcc
     1321 gcagaatgcc gctgtcgtcg acgccgagcg cgcgaaacgc ctttgcgacg cccgcgaacc
     1381 ccgaatcgcc gaacccgtcc tgctgcgcga acacggcgat ttgcctgacc ggtatccggc
     1441 gcaatttcac caggtaacgc accgcggcat cggtttcctc ggcatagctc ggacggtagt
     1501 tgaacacata acgatcggga ggatcatgcc gaacgacatt ggcgccggtg aaggcgccga
     1561 agaacagcat ccgccgctcc agcgcgtatg gcaccgcgac ggccccggtg ggcgtaccgg
     1621 tattcccgat gatgccgaag atctgttcct tgtcgtagag ctgcgtcatg gcattgaggg
     1681 tgcgcgtggg atcattgccg tcgtcggagg agaccagctt gagcatgcgg ccttccacgc
     1741 cgccggcgtc gttggtccgg ttgaatgcgg tatcgatccc gagcttcatg ttgcgaccaa
     1801 gctctttggt cgggccggta aaggcagccg ctatgccgaa cttgatttcc cggtcggtga
     1861 tgccacggac cgggggggcc accgagaccg gtgcggttgg cgcggtggcg gcgaccgccg
     1921 acgatggcga gatgatggag gatgcgggtg gcgcgggtga agccgacctc ggggcgatcg
     1981 accgctcgag atcggcgagc tgacggtctg ccgctacgca gtcaatcctt cccgtggtca
     2041 cggcgctgcg gccgtccgcg acattgcgat cgagcaacag cgtaaggtcg gaacgctcag
     2101 cctcgttgga cgagttttcc gtgatcacgg ccgtgaactt gtcgacgatg acctggatac
     2161 ggggacgcgc gatgtcccgg cacgccaacg ccgaaccgat aatcggtccg acgcggccgg
     2221 caagatcgcg cacaatgtag gtcttgtcgc cagccgaaac gctcccggtg ccagcgagca
     2281 aggcaccgga tacaaccatc atccaacgaa ggctcatcgc aaagctccca aaaccaaggt
     2341 gcttaagcgc tgttggcgat tagcccctgt gacatttctt gatgcaaact ttatttgtaa
     2401 cacgtcgtca gcacggccgt cgcccgctcc gggcgacggc cgtgatatca ttcaagttcg
     2461 atagcctggt acttgccgct ctcatcgagc gccgttcccc agatcttgtg tgaagcctgg
     2521 tgttcggcgc ggccgaaacc gagttgggct ccgagaccca gatcgagctg gcgggtattc
     2581 tccaggacgt cgatgagctt ctcggtatcg agttgcggtc cgcaacgctt aagcgcctgg
     2641 atcaggatat ttgccgaaac ataaccctcg agcgacacgt aatccggcgc ctcgccggga
     2701 aaatatttgg cgatggcagt cttgtattcg agaacgatgc ttgaatagcc ggaaaccgac
     2761 ggcacgacct gcgttacgat tgcgcctgag gcatagcgcg gtcccagcag catcagttca
     2821 tccgcaaggg cggtggcgcc gacgaacgat atgttgctgt agagcattcc aggaaacagg
     2881 tcgcgcgtct tctcgatgaa cctcgcggcg ggtcggtagg ccgaaatcat gaccacagcc
     2941 ttgatcggca ccttctgtgc cttcagctga ttgacggcat cctcgacttc cacggtgttt
     3001 cgctcgtagt tgagccgcag aatgccgctg tcgctgaagc cgagcgaacg aaacgacttt
     3061 gcgacccctg cgaaccccgc atcgccgccc gcatcatgct gggcgaaaac ggcgatctgc
     3121 ctgggcggga tccggcgcat tttgacaaaa tagcgtaccg cggcgtcggt ttcctcggcg
     3181 tagcttgccc gatagttgaa tacatagcga tcgggaggat catggcggat cacattcgcg
     3241 cccgtgaagc cgccgaagaa cagcatccgc cgctccagcg cgtacggcac cgcaacggct
     3301 gcggtcgcgg ttccgacatt cccaataaga ccgaagacct ggtctttctc gtagagttgc
     3361 ttcatggcct cggcggtgcg cgtcggctca aagccgtcgt cggccgcgat cagcttgagc
     3421 atccggcctt cgacgccgcc ggcgtcgttg gtccggttga aagcagcgtc gatcccgagc
     3481 ttcatctggc gcccgagctc ttttgaagag ccggaaaacg gtcccgcaat tccgaaccgg
     3541 atttcccgat cggtcacgcc gcggaccggg gggccggcga tggcagcgac cggcgcggtt
     3601 ggtgctgtgg cggcggcggt ggcgaaagac ggcgcgatca cggcggccaa acttggtccg
     3661 ggcccggcga tcgatcgttc gagatcaccc agttggcggt ccgccaccct gcaatcaatc
     3721 ctgcccgagg tcacggcgct gcggccgtcc gcgacactgc gatcgagcaa ctgcgtcagg
     3781 tcggagcgct ccgcctcgcc ggacgaggct tccgagatca cagcggtgaa cttatcgacg
     3841 atgacctgaa tacggggccg cgagatgtcc cggcaggcca aggccgaacc aacgatcgga
     3901 ccgacgcggc ccgcgagatc gcgcaccacg tcggtcttgt caccagccgc ggcgcccccg
     3961 gcgccagcga gcaaagcacc gaacacaacc gtcatccaac gcagggtcat cgcacgctcc
     4021 acaacatcaa gccttcacca gacgtcagat atcgtcagac atcagacatc gttgtcatct
     4081 ccgcgaccgg gctagccgat tgcgtgatca gggcgctgtg ggctgcgccg gcttttgctg
     4141 ccattgcacg aactgctgaa gcagcgtttc cgactcttcg cgcgaaacgg gcggcggcgt
     4201 ggtggggggt gatgcgagga tcgcctggaa ttctgcaagc gcgggttttg cgtcgtcgtc
     4261 tttttgacgc ggctgggata gggcggtctt caaagactgc aggaacccgg agaccgacga
     4321 attgtcggcg tccggctgtt gtcgcgaata gtcccgcgaa gcagggacca tgatgacgaa
     4381 gaacagcgcc acgatggccg aaacgccgat ggctgcggcg aagcggccgg cgacgctgag
     4441 gatcgccatt cggcgatcca gttcccgcac gaatcccggc ggctcgttca tcacctgggg
     4501 gtcgagcgga tgccgtagcg actcggcaac cgcttcctcg agcaaagcgt cgaaagaatg
     4561 gggaggcgaa atagggcgcg cgaggctctc gggctttttt tcagcggcag gcgcaggaac
     4621 aggggaaggc gaagcggaca ccggttcaga tcgctcgcgt aaccagcgtg gagcatagta
     4681 caactgatcg ttcgggttga gatggtcctg ctcactactt aggctactca tgacgctact
     4741 ctcctcatca ctcttggtta cttgtttatt ctaccgccgt cacccgttcg gcgccgccat
     4801 aacgggtctg tcgttgtttt ggttaatgga caaatgttcc aagccatgac gttcgtcgcg
     4861 ctgaaaaacc cgcgaccggt ccgaaccaga tgccaagttc ggttggagga tttgaatcat
     4921 aatgtgcgat gcaaaatctg caccgttcgg tttagcgcgc gtcagcaggc gagacatatc
     4981 cccagaccgc gtcgcccatg ccccttccat tgccttatcc cctcggcaat tgtccgcctt
     5041 gaacggtagc ggaacgatct tcggctttta acccgaagat gtcaccggta tttgatggaa
     5101 tggtgactgg acattattag gtccgaaact ggacgaaact gaggtaacat tctgcggtgc
     5161 cgcaattttt ggagcggaac gttagtctct tagcagtcgt ttttgcggct tgccgtctca
     5221 ggtagcggct gattggttcc gaaatccggc gtcagcgaca aatgcgccac aattgtcgcc
     5281 gtgactaggc tgattgaacg gctgattcgc ggtgtcgagg tcggtgtgag gtcacgaaag
     5341 gcttcattaa tcaatcgtta accaagatcg cccatcttaa ctattcaata agaaaggcaa
     5401 gtcgaccatc atggtggcaa gggcggaacc ggtacgtcaa cagatgcgcc ttaggggccg
     5461 gtcttacgtt gcgttcgtgt tttgcccctt cgtaccgatc gtgggctggc ttgaggaaat
     5521 agacgcgacg ctcgctcgct cgcccgggtt ttttgtcggc aaacctgtgg tgcttgacct
     5581 gtccgccgtg gacctcagcc agtcggcgat cgcgcacctc gtcacaagcc tcgaaagccg
     5641 gagcattcgc gtcctcggga tcgagggggt ggctgcggct cacgttacga ccagcatgcc
     5701 gcccttgctt accggcggac gccactgcgt ggttccgcag aacaaaccgg aaaaacccgc
     5761 agccaggccg aagccaacgt ctctgctgct cgaaagcccg gtgcgctccg gtcaatcggt
     5821 tatttttgcc gaaggcgatg tgacagtgct gggttcggtc agttccggcg cggagattgt
     5881 cgccggcggg tcgatccaca tctacgggac gcttcgcggc cgcgcgatgg cgggcgtcaa
     5941 tggcaatcca gccgcgcgga tctattgcca gaaaatcgaa gctgagcttc tggccatcga
     6001 tggctactac cagactgcgg aacagatcga cgcctccgtg cgcaatgggc cagcccaagc
     6061 gtggctcgaa ggcgacgtta tgaaaattac accgttgaat taaccgagtg aattaaccga
     6121 gtgaattaac cgagtaagga gctatagaat ggccaaagtc ctggtcgtga cgtcgggtaa
     6181 gggcggcgtt ggaaagacca cttccacggc ggcgctcggt gcggcgctcg cgcaaagcgg
     6241 agaaaaggtc gtggtcgtcg acttcgacgt cggcctgcgc aacctggacc ttgtcatggg
     6301 ggccgaacga cgggtggtgt tcgacctcat caatgtggtg cagggcgttg ccaagctttc
     6361 gcaggcgctg attcgcgaca agcggctgga gaatttgtgg ctgctgccgg cgtcccagac
     6421 cagggacaag gaagccctca ccgacgaggg tgtcgggagc atcatcgcgg agctgcgaac
     6481 caagttcgat tggattctct gcgacagccc ggcgggtatt gagcggggtg cgatgctggc
     6541 gatgcgctat gcggacgagg ctgtcatcat caccaacccc gaagtctcgt cggtgcggga
     6601 ctccgaccgg atcattggga tgctcgattc aaaaaccgtg aaggccgaaa agggcgagct
     6661 ggtgaagaag cacgtgctga tcacccgcta tgatacggcc cgcgcagcgc ggggcgaaat
     6721 gctcaccatc gaggacattc ttgaaatcct cgccaccccg cttctcggca tcatccccga
     6781 gagccaggac gttttgcgtg cgtcgaacgt cggatctccc gtcaccttga gcaacgccgc
     6841 gagtgcgccg gcccgcgcct acaccgatgc agcccgtcgc ctgatgggcg agaccgtcac
     6901 catgctggtg ccggccgagc gcaagggctt tatggaccga ttgctgggac ggagggccgc
     6961 atgagcgcca gtctgctgcg gttcttcagt ggccgcaagt ccgcgcccgc ttctgcttcc
     7021 gcacctatcg cgcgggaacg gttgcagatt ctcctggcgc atgaacgcag catgcgcggg
     7081 gagcctgact tgctggtgct gctgcgggac gaaattcttg ccgtggtgtc gcgacatgtc
     7141 acgctcgatc cggaccgggt gatcgtcaag atggacaggg gcgacacggt gtcgacgctt
     7201 gaggtcgata tcgaactgcc caacggattc gagaggatcg cggctgctgg ctagcgatcg
     7261 ggcgcaccgg ctgcgcttag ctctcttgcc cggatatcgg cgtcgcaacg cgataaattc
     7321 catgataatc cttcgatcgc agcttcgatc ggaggtcgat catgagcccg aagcgacctg
     7381 cgcttccgat gaccggcggc tgtccatgcg gtgtcatccg ctaccagatc acctcgtttc
     7441 cactgctgct ttacacctgc aattgtaccg attgtcagag ggcttccggc agcgcgttcg
     7501 cgttgaacat gccggtccgt gccagggatt ttcatatcct gcagggcgag ccgaagggat
     7561 ggcgccacac gtcgccgacc ggggtcgccg tgatatcctg gttttgcggt gattgcggcg
     7621 gccgcctcta cggcgaccga gccgcgcgtg aggccatcgt caacctccgt gccggaacgc
     7681 tggacgatac gacatggctg gttccgatcg ctcatatgta cacgagaagc gcgcaaccat
     7741 gggtcgcacc cgcggtcaat gccgagtgtt acgagatcgg ccccgacgac ttccggccgt
     7801 tggccgagcg atggcgcgcg atgtggcctg aattcctgct ggaaaaatag ttccattttc
     7861 tcggaaccgt tggaggtcag acaggtttag ccccggttag gggggataat gaacttggtt
     7921 gaacccacca ccagcaaggc gaaaaccggg atttggggac tggacgacat cctgtcgggc
     7981 ggattttcgc gcgggcacat gtttctggtg gagggcgcgc cggggaccgg caaaaccacc
     8041 attgcgctgc agttcttgat ggaaggcgcg caggccggcg agaagtgcct ctacatcacc
     8101 ctgtccgaaa ccgagcgcga gttgcgcgaa ggtgcggcct cccatggctg ggcattagag
     8161 gacggcattg aagttttcga gttgttgccg ccggagagtc tgctcgattc cgagcagcag
     8221 cagagcctgc tttattcttc cgatctcgaa ctcggcgaaa cgaccaaaca gattttcgga
     8281 gccgtggaac gcgcacggcc gagccgggtg gtgctggaca gcctctccga gatccggctg
     8341 ctggcgcaaa gttcgcttcg ctaccggcgg cagattctcg cgatcaagca ctatttcgca
     8401 aagttcggca ccacggtgat gctgctcgac gatttgaccg ccgagcttgc cgacaaaacc
     8461 gtccacagcg tggcgcatgg tgtgctgcgg ctcgaggaat tggcgccggc ctatggcgcg
     8521 gagcggcggc gcgcgcgcat cgtcaaatat cgcggcgtca tgtttcgcgg cggctatcac
     8581 gatgcaacca tcaccaccgg tgggctgaat atctttccgc ggctggtcgc ctcggaatac
     8641 cgcagcagct ttgtacgaac caggatgtcc agcggcatcg ccgagttgga ccggcttctc
     8701 ggtggcggca tcgaagccgg atcgagcacg cttgttcttg ggcccgcggg caccgggaaa
     8761 tcccttttgg cgatcgtctt cattgccgcc gcgatcgcgc gtggcgaaaa ggcggcgctg
     8821 ttcgtgttcg acgaggagct cggtctgttg tttgcacgca tgaaggggct cggcattgac
     8881 ctcgaagcga tgcagcgcag cggcaacctc ttcgtcgagc aggtcgacgc cgcggagctg
     8941 ttgcccggcg aattcgcgca ccgcgtccgc aagcgggtcg atgaggacga catcaagacg
     9001 gtggtgatcg acagcctcaa cggttatcag gccgcgatgc ccgaggaaaa ctcgctgatc
     9061 ctgcacatgc acgaattgct gcaatatctg aaccgccggg gcgctgcgac attcatgacg
     9121 gtggcgcagc atgggctggt cggcgatatg aaggcgccgg tggatgtgac gtatttggcg
     9181 gacaccgtga tgctgttgcg ctatttcgag gcgctcggca cggtgcggcg cgcgatatcg
     9241 atcatcaaga agcgcaccgg cgcgcacgaa tccacgatcc gcgaatatcg catcgacggc
     9301 cgcggcctta ccatcggtga cccgctcgac ggctttcagg gcgtgttgcg cggggtcccg
     9361 gtctacgtcg gcgaaggccg gcccttgctg gaagagcaag tccagtgaaa cccggcgctt
     9421 cctcggagcg tgccgtggtt ctcgcgccga cgggacgcga cgccacggtg gccacggcgc
     9481 tgatcaggga ggcgggttac cacgccaatg tctgcggcga tctcgccgcg ctgttgcacg
     9541 agatcgaagg tggcgccggc cttgcggtga tcgccgacga ggcgatcaag acggccgatc
     9601 tgcgcggcct gatgcgctgg ttgaacgatc agccgtcatg gtcggatttt ccgatcgtgc
     9661 tgctgacgca tcagggcggc ggtcccgaac gcaatccgga cgccatgcgg cttggacaag
     9721 tgctcggcaa cgtcactttc atcgaacgtc cgttccaccc cacaacgctg gtaagcgtgg
     9781 tcggctccgc cgtcagaggc cggcgtcgcc agtaccagac ccgcgccatt cttcaggatc
     9841 tcaccgaaag cgaaggcctg ctgcagaccg cgctgagcgc cggccgtctc ggtgcgctcg
     9901 agctgcatct tccggagctt gagttggagg cctccgacac ctgcaaaacg ttcttcggcc
     9961 gcaagccggg cgagccgttt tcgtaccagg atttgctggc ggcggttcat cccgacgatc
    10021 gcgcgcgacg tctcgaggtg ctggatcggg ccatcgagac cggccgggac tacagcatcg
    10081 agtac
//
LOCUS       DATNOI010000446         5937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_375679,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000446 DATNOI010000000
VERSION     DATNOI010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375679"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="VN968_17905"
     CDS             complement(<1..152)
                     /locus_tag="VN968_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HXL31144.1"
                     /translation="MSEQGSDRFEIPKEMRSMAEASFNQARTAFEKFLAGAQQTAGTI
                     EERGATV"
     gene            536..3766
                     /locus_tag="VN968_17910"
     CDS             536..3766
                     /locus_tag="VN968_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXL31145.1"
                     /translation="MNHAEFQLRGVSDPRLAVHATSATPAWLWSVDGTRILWANPVGA
                     RLFNAAHGAALANKSFGPADLHRRQVARLAGRLPSNGAIRLERLRGFGAALGALVTCG
                     CARLDFADGGHGILIASAEAAGRTMPLVERLQRLVEGIDAPIAALTRDGIFVGASTAA
                     RSLLGFRNLAEVGLDDACSEALKQGRVETPVGIGHLILQRIGTGADVGLVALIAPGAT
                     QAAHTRHTAPEASVAEPAIVPAADLPLPNDEQSAMSGEAPAEFGLIDEFAESPSEAAV
                     EPMRLDQPPEPDEPPPTAPEHPLRFLWQLTAEGRFSLAPGEFTRLIGARTTAAFERPW
                     SEIAERFGLDPDGRFLNAIATRGTWTGVTLNWPADGGGRLPVELSGLPIYDRAANFAG
                     YRGVGVCSDLDGMARLAALRRDEFVSHPPAPQTLSAVIVQPKSADIVRTYIGDSLAAE
                     LAAKPDVASSDTPESPESNALESNASETSQQTDLETAVDTPKNVLPFRPVSDPKSPAL
                     TQVENSAFNELARQLSARLDNENGAAVAMPVATETVDERPSTPETPEPPSEPPEWLAP
                     PEEPARGEARRDKALLDLLPVGVLIYRLDRLLYANRAFLVRMGYESLHALEDAGGLDA
                     LYVEPGVSSASSTSDAGRPVKISGMEASSEHTPAVATDARLFTISWDGDPALALIFSS
                     TRTEAAAVAAAFSEAIPAAAPSAAGHANAEELSAILDTTAEGIVMFDAEGTIHSCNRS
                     AEALFGYDGSQLVQRNLMQLFAPESQPAVRDYLEGIKGADVASLLDHGRDVLARVRKG
                     GIIPLSMTMGRTRPDGPNFFAVFRELSQNRTSESEPQQAGRLADRAANAKADLLARIS
                     HEVRTPLNAIIGFSEVMIAERFGALNERYIEYMKDIRSSGERVIAIVNDLLDLSRIET
                     GRLDLTFASQNLNDLVEQCVAVMQPQANRERIIIRTSLAPMLPPVIADARALRQIALN
                     LIGNSIHLANAGGQVIVSTALSDFDEVVLRVRDTGQGLNEKEVAAAMEPFRTQPPSDQ
                     ATVNSGINLSLTKALVEANRAHFHIKTASHSGTLIEVTFSHATAKA"
     gene            complement(3908..4099)
                     /locus_tag="VN968_17915"
     CDS             complement(3908..4099)
                     /locus_tag="VN968_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31146.1"
                     /translation="MKVKIFMDASASTIEAQINTWLDGIDAAVIVKTDTVVTNVAEKA
                     GDGAYPCIVVTIWYEPPQS"
     gene            complement(4160..4411)
                     /locus_tag="VN968_17920"
     CDS             complement(4160..4411)
                     /locus_tag="VN968_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31147.1"
                     /translation="MKELSLKGELDYKMISVQCTYCKTPIGIIDDNNALLQQQQARIL
                     RVERRLSSMEDKLSDIEEKLSEIASALTQRGFKVGKVER"
     gene            5000..5827
                     /locus_tag="VN968_17925"
     CDS             5000..5827
                     /locus_tag="VN968_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXL31148.1"
                     /translation="MRCLVVADLHYSLPQFDWLLSAAPQFDLVIFAGDALDVGSSVDF
                     RAQIVVVKKYLALLSGLTRVILCSGNHDLDERNPEGEKIARWIGDVRELGIACDGDSL
                     TIGDTFFTVCPWWDGPLVKQRIEDQLREAAAGRLKRWIWVHHAPPANSPTSWGGKRFF
                     GDEELVQWIRSYQPSMVISGHVHQSPFIADGSWFDQLGSTWVFNAGLQPGRPPVHIVL
                     DIDDGTAFWLPLGSEQYIDLNAPLQRPAAAITHPPEWLTSLGRIADPNLARSLSAAG"
     gene            complement(5766..>5937)
                     /locus_tag="VN968_17930"
     CDS             complement(5766..>5937)
                     /locus_tag="VN968_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXL31149.1"
                     /translation="LAQRLNVATTYLADIMQQYAGHGDHLAMVGEILQTMINLPPITI
                     SPGSDRQSDPRM"
BASE COUNT         1079 a         1923 c         1731 g         1204 t
ORIGIN      
        1 accgtcgcac cccgctcctc gatggttcca gcggtctgct gcgcgccggc aaggaatttc
       61 tcaaaggcgg tgcgggcctg attgaagctg gcctcggcca tcgatcgcat ctccttcgga
      121 atctcgaaac ggtcactccc ctgttcgctc atggtttcac tccctgttgc ttatcgcagc
      181 cccacagctg ccccgattcc gggttcgcca cccacacgat cggccaacgc cttcgccgtt
      241 atgacacggc gtcccttggt tcccatcgcg gacttgtggt ggcttttttc aacaccccaa
      301 cgcgagagcc ggcgcggagt tgcaaggccg ggttcggaaa ttctcgttgg tcgcggttgc
      361 agctgttggc gctgctaatg ggggttatcc ccgccatagg atgcctcctc gagggcaagg
      421 ccgtggacct cccgtctgca gcgggcgata ctaacggtct gttaaggctg tcatcgtttc
      481 ggccgccgat atctgaggcc gcgtaacagc caaaccagcc ggtcatgccc ctgcgatgaa
      541 ccatgcggaa ttccagttgc gaggcgtcag cgatccgcga ctggcggtgc atgccacaag
      601 cgccaccccc gcatggctct ggtcggtcga cggcacgcgg attctttggg ccaatccggt
      661 cggcgccagg ctgttcaatg cagcccatgg cgcagcgctt gcgaacaaga gtttcggacc
      721 ggcggatttg caccggcgcc aggtcgcgcg tctggcgggc aggctgccat cgaacggcgc
      781 gattcggctg gagcggctgc gcggcttcgg cgcagcactc ggtgcgctcg ttacctgcgg
      841 ctgcgcacgg cttgattttg ccgatggcgg ccacggcatc ctgattgcga gcgccgaagc
      901 ggccggccgc acgatgccgc tggtcgagcg attgcagcgc ctggtcgagg gcatcgatgc
      961 gccgatcgcg gcgctcacac gcgacggcat attcgtcggc gccagcactg ccgcgcgatc
     1021 gctgctcggc tttcgcaatc tcgccgaggt tggccttgac gatgcgtgca gcgaggccct
     1081 gaagcagggc cgcgtcgaga cgcccgtcgg catcggccac ctgatcctgc aacgcatcgg
     1141 gaccggcgct gatgtcggcc tcgtggcact gatcgcgccc ggcgcgacgc aggccgcaca
     1201 caccaggcat actgcgccgg aagcctcggt ggccgaacct gcaatcgtac ccgcggcgga
     1261 tctgccgctc cccaacgacg agcaatcagc gatgtcaggc gaagcaccgg cggagtttgg
     1321 gttgatcgac gaatttgcgg aatcgccctc cgaggctgcc gtcgaaccca tgcgcttgga
     1381 ccagccgccc gaacccgacg agcctccgcc gaccgcgccc gagcacccgc tgcgcttcct
     1441 gtggcagctc accgccgaag gccgcttctc gctcgcccca ggcgaattca cccgcctgat
     1501 cggcgcccgt actaccgccg ccttcgaacg gccatggagc gaaatcgccg agcggttcgg
     1561 gctcgatccc gacggacgct tcctcaacgc gatcgcgaca cgcggcacct ggaccggcgt
     1621 taccttgaac tggcccgccg atggcggcgg ccgattgccg gtggaattgt cgggattgcc
     1681 gatctacgac cgcgcggcga attttgccgg ctatcgaggc gtcggggttt gcagcgatct
     1741 cgacggcatg gcgcggcttg ccgcgttgcg ccgcgacgag ttcgtcagcc atccgccggc
     1801 accccaaacg ctttcggccg tcatcgtcca gccaaaatcg gccgacatcg ttcggacata
     1861 tattggagat tcgcttgccg cggagctggc tgcgaagcct gatgtcgcat cgtccgatac
     1921 gcctgaatcg cccgagtcga atgctcttga gtcgaatgct tctgagacat cacaacaaac
     1981 cgatctggaa accgccgtgg atacgcccaa gaacgtcctt ccgtttcggc cggtgagcga
     2041 tccgaaatcc cctgccctga cacaggtgga aaacagcgcc ttcaacgaac tcgcccggca
     2101 attgtcggcg cggctcgaca acgaaaatgg tgctgccgtc gcgatgcctg tcgcgaccga
     2161 aaccgtcgac gagcggccgt cgacgcctga aacacccgaa ccaccgagcg aaccacccga
     2221 atggctggcg ccgcccgagg agccggcgcg gggcgaagcc cggcgcgaca aggcgctgct
     2281 cgatttgctg ccggtcggcg tgctgattta ccggctcgac cggctgctct atgccaaccg
     2341 cgcgtttctg gtgcggatgg gctacgagag ccttcacgca ctggaagatg ccggaggtct
     2401 cgacgcgctc tatgtcgagc ccggcgtctc gagcgccagc agcacatcgg acgccggcag
     2461 gccggtgaag atttccggaa tggaggcttc ctccgaacat acgccggcgg tggcgaccga
     2521 cgcgcgcctg ttcacgattt catgggacgg cgatcccgcg ctggctctga ttttctcgag
     2581 cacacgcacc gaagccgccg cggtcgccgc cgcattttcc gaggcgatcc ctgccgccgc
     2641 accgtcggcc gccggccacg ccaacgccga ggagctcagc gccatcctcg ataccaccgc
     2701 cgaaggcatc gtgatgttcg acgccgaggg caccatccat tcgtgcaacc gcagcgccga
     2761 ggcgctgttc ggctatgacg gctcgcagct cgtgcaacgc aatctgatgc agctgttcgc
     2821 gccggaaagc cagcctgcgg tgcgggatta tctggagggc atcaagggcg ccgatgtcgc
     2881 gagcctgctc gatcatggcc gcgacgtact cgcccgtgtg cgcaagggcg gcatcatccc
     2941 gctgtcgatg accatgggac gaacccggcc cgacggcccg aatttcttcg cggtgtttcg
     3001 cgaactttcg cagaaccgca ccagcgagag cgaaccgcag caagcggggc ggctggccga
     3061 tcgcgcggcg aacgccaagg ccgacctgct ggcgcggatc agccacgagg tccgcacccc
     3121 gctcaacgcc atcatcggat tttccgaggt gatgatcgcc gagcgtttcg gcgcgctcaa
     3181 cgagcgctac atcgaataca tgaaggacat ccgcagctcg ggcgaacgcg tgatcgccat
     3241 cgtcaacgac ctgctggatc tgtcgcggat cgaaaccggc aggctcgacc tcacctttgc
     3301 cagccagaac ctcaacgatc tggtcgaaca atgcgtcgcg gtgatgcagc cgcaggccaa
     3361 tcgcgaacgt atcatcatcc ggacatcgct ggcgccaatg ctgccgccgg tgatcgccga
     3421 cgcccgcgcg ttacgtcaga tcgcgctgaa cctgatcggc aactccatcc atctcgccaa
     3481 tgccggcggc caggtcatcg tctccaccgc gctgtcggat ttcgacgagg tggtgctgcg
     3541 ggtccgagat accggccagg ggctcaacga gaaagaagtc gccgcagcga tggagccgtt
     3601 ccggacccag ccgccgtcag accaggcgac cgtcaattcc ggcatcaacc tttccctgac
     3661 caaagccctg gttgaagcca accgcgcgca ttttcacatc aagaccgctt cgcattcggg
     3721 cacactgatc gaagtgacgt tctcccacgc aaccgcaaaa gcctagagcc tgatggcttt
     3781 gagttgaatc ggcgagtact cggatcacct caccccgctt caacctaaac tcataatata
     3841 aagtctaaag acttcgtggt ttctcggtat caatgaggtt gctgtagtcc cggcgatgtc
     3901 ggtcagacta gctttgcggg ggctcatacc aaatcgtcac gacgatgcac ggataagcac
     3961 catcaccagc tttctcggct acgttggtaa ccacggtatc tgtctttacg atcacagccg
     4021 cgtcgatgcc gtcgagccat gtattaatct gcgcttcgat ggtgctcgcg ctagcgtcca
     4081 tgaagatttt tactttcatt ggctttggca tccgacagcc ttccgatggt tgatgactga
     4141 tgataggttc gcgcgcgacc taccgctcta ccttgcctac cttaaatccg cgctgcgtta
     4201 gagcagacgc gatttcagaa agtttttctt ctatatcaga aagtttatct tccatcgatg
     4261 agagccttcg ttcaacacgc aatatccgcg cttgctgctg ttgcaggaga gcgttgttgt
     4321 cgtcaataat cccgattggc gtcttacagt atgtgcactg taccgaaatc attttgtaat
     4381 ccagctcgcc cttcaaactc agttctttca ccttgaaggt actcttctcg cacttgccgc
     4441 acctcgtcgt agcgaacccg aacataagaa tctccagttg tttgcgtgat tcgccacaac
     4501 gtgagtcgta ttggaactta agtattggct tttccggggc ggctaaagcc taccaaagcc
     4561 ggcgaaactg gcgtacgcgg gccgtcaaag acggcctatc gtctggtagt tcaccccgaa
     4621 caaccggccg atctctgacg ggctttcccc tgcatcacgc cgtcgcttag cctcttgttg
     4681 ctgatgaggc gtcagcgtgt gcttcctgcc gaacttcact ccccttgcca tagcctagct
     4741 ctgccctcac cggtccgcgt cagacgcgcg gtagccatag atggtcatca gaacctcact
     4801 tttggttatg acatatggtt agttgaacca gttgatttca cttcgctttt ccatctaccc
     4861 aaaacttatc acgctacgtt cgtgcgggca tctgtcgcac tgcaacaagg tggcgcggca
     4921 taatttttgc tcgctcctgg ctgtttgaat acagccctct ctacatcgcc tagtttttaa
     4981 gcatccggat tgagttgata tgcgctgcct tgtcgtggcc gatttgcatt attcattgcc
     5041 gcaatttgac tggctgctca gcgcggcgcc gcagttcgat cttgtgatct ttgcgggcga
     5101 cgccctcgac gtcggatcct cggtcgattt ccgcgcccag attgtcgtcg tgaagaaata
     5161 tctggcgctt ctctccggcc tcacccgggt gattctttgc tccggaaatc acgatctcga
     5221 cgaacgcaat ccggaaggcg agaaaatcgc gcgctggatc ggcgacgttc gcgaactcgg
     5281 catcgcctgc gacggcgaca gcctgaccat cggcgacacc ttttttacgg tctgtccctg
     5341 gtgggacgga ccgctggtca agcagcgtat cgaggaccag cttcgcgaag ctgccgcagg
     5401 ccggctcaag cgatggattt gggtccatca cgcaccgccg gcgaattctc cgaccagttg
     5461 gggcggtaaa cgctttttcg gcgacgaaga actggtgcag tggatccgga gctatcagcc
     5521 ttcgatggtg atctcgggcc atgtgcatca atcgccgttt attgccgacg gctcctggtt
     5581 cgatcagctc ggcagcactt gggtgttcaa cgccggcctt caacccggcc gcccgccggt
     5641 gcatatcgtg ctcgatatcg acgacggcac cgccttctgg ctgccgctcg gctccgagca
     5701 atacattgac ctcaatgcgc cgttgcagcg gccggctgcg gcgatcaccc atcctccgga
     5761 gtggctcaca tccttgggtc ggattgccga tccgaacctg gcgagatcgt tatcggcggc
     5821 aggttgatca tggtctgcag aatctcgccg accatcgcga ggtgatcgcc gtggccggca
     5881 tattgctgca tgatgtcggc aagataggtg gtcgcgacat tgagcctctg ggccagc
//
LOCUS       DATNOI010000447         6588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2159650,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000447 DATNOI010000000
VERSION     DATNOI010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2159650"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2590
                     /locus_tag="VN968_17935"
     CDS             <1..2590
                     /locus_tag="VN968_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXL31150.1"
                     /translation="VGEVTVSGADQPAVRIALNPVALANAGIATDDVRTAIINANPLG
                     PVGIFNGDRQSETLAMNKQMRTAAEFRDIMIKSANGNFVRLSDVAEVEDSVRNNRSIA
                     WFNKQPAVLIQITKQGDANVIDTVDRVHALIPELKQWIPAGVDISTLVDRTGTIRASL
                     ADMQLTLLATAILVMVVVFAFLRRITPTIAAGVSVPLALAGTCAGMWLAGFSIDNLSL
                     MALAISVGFVVDDAIVMIENMYRNLEHGMRPYQAALEGARQIGFTVLSISLSLIAAFT
                     PLIFMDGIVGRLLREFSLTLTFAIVVSTVVSLTITPMICAHYIKQVTSHNATWFDRVV
                     EGSLSRIVAFYARTLRVVLGFPFLTLMVFFATIALTVTLYIKTPKGYFPTDDSGFVIG
                     ATRASADISFQSMLGLQQQLADIVMADPAVAGIGSVLGGGGGPGGATSNRGMMFISLK
                     PPSERAGLSTADVIDRLRKNLFRVAGIRLFMFAAQDIRAGGRQSDSDYQYTLTSTDLD
                     LLQKWAPIVGKRMESIEGITDVSSDRDPGGLQLTLSIDRKAASSLGVRVQDIDNALNN
                     AFSQRQISIVYTQRNQYMVVLEIDPKFQSDPSNLEHIFVAGAGGAQVPLSAVVHYQRG
                     LSALAVFHSQSFPSTTVSFNLLPNVPLEVATSNIQRAVEELHMPEGIRGSFDGNAGDF
                     GKTSGHMPLLILGALVAMYIVLGVLYESLAHPLTIISTLPSAGLGALLALQVTNTPLT
                     VIAFVGIILLIGIVKKNGIMMVDFALDAERHRGLSSADAIFEACSARFRPILMTTMAA
                     LFAGIPLVIATGPGTELRRPLGITIIGGLFVSQILTLYTTPVIYLLIDRLRRRSGPAA
                     IAAPAE"
     gene            2663..3397
                     /locus_tag="VN968_17940"
     CDS             2663..3397
                     /locus_tag="VN968_17940"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HXL31151.1"
                     /translation="MSKQSEPETGAAAEAIPDCPHCQKPVPLCICDSITPIENKVALL
                     ILQHPQEQDRALGTARLTALHFANAVLKIGLSWPSLSKALGRSVHDPSRWAVLYLGSA
                     KVAELDIDRDIVAVNRKGEIEEHQRVILKDIEGIVLLDGTWSQAKALWWRNAWMLKCQ
                     RVILGPRHPSRYGKLRREPRRDGLSTIEAAAMLLSNLEKRPDIAETLHGSFERMLAKY
                     REAQSVLPELAPKPKPKRDYRRRKRA"
     gene            3588..4376
                     /locus_tag="VN968_17945"
     CDS             3588..4376
                     /locus_tag="VN968_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HXL31152.1"
                     /translation="MKRIVGAVMVTAALTAPALAADLGARSYTKAPALAPVRNWSGFY
                     IGGNLGYGWRNGNTDFSFLPTPAAFGDNNGSLGIRSTGVIGGAQIGYNWQIGPLVTGI
                     EADVQGSDIKGSARATATFFGTAVPDPQVVPSSETKLAWFGTVRGRLGVTVTPDLLLY
                     GTGGLAYGRVDASANTHLFVPDGSPFAESPASVSKTKVGWTAGAGAEWMFARNWSAKL
                     EYLYLDLGSDSAIGNVTPATGFQVGYTWHTRENITRVGVNYHFD"
     gene            4634..4707
                     /locus_tag="VN968_17950"
     tRNA            4634..4707
                     /locus_tag="VN968_17950"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4666..4668,aa:Cys,seq:gca)
     gene            complement(4784..5230)
                     /locus_tag="VN968_17955"
     CDS             complement(4784..5230)
                     /locus_tag="VN968_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31153.1"
                     /translation="MPLMVSVVLDRLKSKLAMFCVQAFEVKSGMPAANPAYPLASYLL
                     ARHGHKSTGKRSENYSGRDTKSPESTSVMVGADPLAARELPIAIILPRQGNFLPNLTA
                     SGRSFYLAASLPSPRKPLAIQPETTSDSDAGHPRPIFQKMRHDAGG"
     gene            5248..5505
                     /locus_tag="VN968_17960"
     CDS             5248..5505
                     /locus_tag="VN968_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31154.1"
                     /translation="MPKKGITGRDEWVVTEALATALVALEQLPPKHQPRTHMEDIRKL
                     LAAGCQPGTVTLHLAQAKCRLFPDVDPLTIYEEYGLGDGQG"
     gene            complement(5623..5802)
                     /locus_tag="VN968_17965"
     CDS             complement(5623..5802)
                     /locus_tag="VN968_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31155.1"
                     /translation="MSEVEFDCLLDAVRTAIAPAPEEDFLAHPSTFNQLPKAANDNKS
                     AWPLIPFPEGWNAVS"
     gene            6052..6306
                     /locus_tag="VN968_17970"
     CDS             6052..6306
                     /locus_tag="VN968_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31156.1"
                     /translation="MNSRSLLAATRGRTNAAVALALLLALFTTGSALAATATPEQKKA
                     CTPDVYRLCAGEIPNVRAIISCLRRQKASLSEACRAVFEP"
BASE COUNT         1269 a         1977 c         2050 g         1292 t
ORIGIN      
        1 tgtcggcgag gtgacggtga gcggcgccga ccagccggcg gtcaggatcg cgctcaaccc
       61 ggtggcgctg gcgaacgccg gtattgcgac cgacgacgtc aggaccgcga tcatcaatgc
      121 caatccgctc gggccggtcg ggattttcaa cggcgatcgc cagagcgaaa cgctcgcgat
      181 gaacaagcag atgcgaaccg cggccgaatt tcgcgatatc atgatcaaga gtgcgaacgg
      241 caatttcgtc cggctctctg acgtcgccga ggtcgaagac tccgtccgca acaaccggtc
      301 gattgcctgg ttcaacaagc agccggcggt gctgatccag atcaccaagc agggcgacgc
      361 caacgtcatc gataccgtcg atcgcgtgca cgcgctgata cccgaactga agcaatggat
      421 tccggccggg gtcgacattt cgacgctggt cgaccgcacc ggaaccatcc gcgccagcct
      481 agcggacatg cagctgacgc tgctggcgac cgccatcctg gtcatggtgg tggtgttcgc
      541 attcctgcgc cggatcacgc cgacgatcgc ggcgggcgtc tcggtgccgc tggccttggc
      601 cggcacctgc gccgggatgt ggctcgccgg tttctcgatc gacaacctgt cgctgatggc
      661 gctggcgatt tcggtcggct tcgtggtcga tgatgccatc gtgatgatcg agaacatgta
      721 ccgcaacctc gagcatggca tgcggcccta tcaggccgcc ctcgaaggcg ccaggcagat
      781 cggcttcacg gtgctgtcga tcagcctgtc gctgatcgca gccttcaccc cgctgatttt
      841 catggacggc atcgtcggcc ggctgttgcg ggaattttcg ctgaccttga cgtttgcgat
      901 cgtggtttca accgtggtgt cgctgacgat cacgccgatg atctgtgccc actacatcaa
      961 gcaggtcact tcccacaatg cgacctggtt cgaccgcgtt gtcgaaggct cgttgtcgcg
     1021 catcgtggcg ttctatgcgc ggacgctgcg ggtggtgctg ggttttccgt ttttgacgct
     1081 gatggtcttc ttcgcgacca tcgcattgac ggtgacgctc tatatcaaga cccccaaggg
     1141 atattttccg accgacgact caggttttgt gatcggcgcc acgcgggcat ccgccgatat
     1201 ctcgtttcag tcgatgctgg ggctgcagca gcagctggcg gacatcgtga tggctgatcc
     1261 ggccgtggcg gggatcggat cggtgcttgg cggaggcggc ggccccggcg gggcgacctc
     1321 caatcgcggc atgatgttca tctcgctcaa gccgccatcc gagcgggctg gattgagcac
     1381 cgcggacgtg atcgatcggt tgcgcaagaa tctctttagg gtggcaggga tccggctgtt
     1441 catgttcgcg gcgcaggata tccgcgccgg cggccgacag agcgattccg actatcaata
     1501 cacgctgacg agcactgatc tcgatctgtt gcagaaatgg gcgccgatcg tgggcaaacg
     1561 catggagagc attgaaggca ttaccgacgt ctccagcgac cgcgatcccg gtggcctgca
     1621 attgacgctg tcgatcgacc gaaaagcggc atcgagcctc ggcgtgcgcg tgcaggacat
     1681 cgacaacgcg ctcaacaacg cgttctcgca gcggcagatc tcgatcgtct atacccagcg
     1741 caaccagtac atggtggtgc tggagatcga tccgaaattc cagagcgatc cgtccaacct
     1801 cgaacatatc ttcgtcgccg gcgcgggcgg cgcccaggtg ccgctgtccg ccgtggtgca
     1861 ttaccagcgc gggttgtcgg cgctggcggt gtttcattcg cagtcgtttc cgtcgaccac
     1921 ggtgtcattc aatctgctgc ccaacgtgcc gctggaggtc gccacatcga acatccagcg
     1981 cgcggtcgag gaattgcaca tgcccgaagg cattcgcggc agctttgacg gcaatgccgg
     2041 cgatttcggc aagaccagcg ggcatatgcc gctcttgatc ctcggcgcac tggtagcgat
     2101 gtatatcgtg ctcggggtgc tgtacgaaag cctcgcccat cccctcacca tcatctcgac
     2161 cttgccgtcg gccggcctcg gcgcgctgtt ggcgctgcag gtgaccaaca cgccgctgac
     2221 ggtcattgcc tttgtcggca tcattctcct gatcggcatc gtcaagaaga acggcatcat
     2281 gatggtggat tttgccctcg acgcggaacg tcaccgcggc ttgtcgtcgg cggacgccat
     2341 cttcgaggcc tgcagcgccc ggttccgtcc gattctgatg acgacgatgg cagcactgtt
     2401 cgccggcatt ccgctggtga tcgccaccgg ccccggcacc gaactgcgcc ggccgctcgg
     2461 catcaccatc atcggcggat tgttcgtctc gcagatcctg acgctgtaca cgacgccggt
     2521 gatctatctc ttgatcgacc ggctgcggcg acggtccggg cccgccgcga tcgcggcgcc
     2581 cgcggaatag cgccctcaga ttggcttgag cgatcgacct tgagtggttg aagcgcacgt
     2641 cctcagagcg tatagcgggc ggatgtcaaa acaatccgag cctgagaccg gcgccgcggc
     2701 ggaggcgatc ccggactgtc cgcactgcca aaaacccgtg ccgctgtgca tttgcgacag
     2761 catcacgccg atcgagaaca aggttgcgct gttgatcctg cagcatccgc aggagcagga
     2821 cagggcgctc ggcacggcga ggctgaccgc gctgcatttt gccaatgcgg tgctgaaaat
     2881 cggtctgtcg tggccgagcc tgtccaaggc gctggggcga tctgtccacg atccctcgcg
     2941 ctgggcggtg ctttacctcg gttcggcaaa agtcgccgag cttgatatcg accgggatat
     3001 cgtcgcggtc aaccgcaagg gcgagatcga ggagcaccag cgcgtcatcc tgaaagacat
     3061 cgagggcatc gtgctgctcg acggcacctg gagccaggcc aaggcgctgt ggtggcgcaa
     3121 tgcctggatg ctgaaatgcc agcgcgtcat tctcggcccc aggcatccgt cgcgctacgg
     3181 aaaattgcgc cgcgagccgc gccgcgacgg cctgtccacc atcgaggccg cggcgatgct
     3241 gctgtctaat ctcgaaaagc ggcccgacat cgccgagacg ctgcacggca gttttgagcg
     3301 gatgctggca aaataccgcg aggcccaaag cgtactgccc gaactcgcgc ccaaaccaaa
     3361 gcccaagcgc gactaccgcc gccgcaagcg cgcttgatcg ggtgctgggc gcgctggcat
     3421 cgacaaaaat gcatcagtgg ccgtcaatcg ccagaagtgc gttttgggca acgcgaccgt
     3481 cgcggctgtc caagtcgcag aacccgcttc cccgtagatc ttgcgccgag attcgagagc
     3541 accgcgcgcg cggcgctctc tcagggctcg attgcaaggg ggttatgatg aaacggattg
     3601 tgggtgcggt gatggtgacg gcggcgctga cggcgccggc tttggcggcc gatcttggtg
     3661 cgcggagcta taccaaggcg ccggcgctgg cgccggtcag aaattggagc ggcttctata
     3721 tcggcggcaa cctcggctat ggctggcgca acggaaatac ggatttctcg ttcttgccaa
     3781 ctcctgcggc tttcggagac aacaatggga gcttggggat ccgatcgacc ggcgtcatcg
     3841 gtggcgcgca gatcggttac aactggcaga tcggccctct ggtgaccggc atcgaggcgg
     3901 atgtccaggg ctcggacatc aaaggctcag ctcgcgcgac agctaccttt tttggcactg
     3961 cggttccgga tccgcaagtg gtcccttcgt ccgaaacaaa gcttgcatgg ttcggcacgg
     4021 tgcggggtcg gctcggcgtc accgtcacac ccgacctgtt gctctatggc accggcggtc
     4081 tggcctatgg ccgcgtggac gcttcggcaa acacccactt gttcgttccg gatggaagtc
     4141 ccttcgctga gtcgcctgca agtgtcagca agaccaaggt cggctggacc gccggcgccg
     4201 gcgccgaatg gatgttcgca cgtaattggt cggccaagct ggagtacctt taccttgacc
     4261 tcggcagtga ctctgcgatt ggaaatgtta ctccggcaac tggattccag gttggataca
     4321 cttggcacac ccgggaaaac atcacccgcg tcggcgtgaa ctaccacttc gactgacggc
     4381 gtccgcgcga ccgcctgaac ccgtttcgag gaaagcccgg caagttgccg ggctttttcc
     4441 ggaaaatcat cgtggccagg ggattgccgt gcctggctga ccctgccgac ccggcgagag
     4501 cgagctctgt tggcagttcc gccgcggcgg aagcgccatt tcggcattga gaccggactt
     4561 cagaaattgg cgaccaacgc ggcggaggag gttgcctaaa cccttgatgg tctgtagcaa
     4621 gtcggtccca aggggccacg tggcggagtg gttacgcaac ggtctgcaaa accgtgtaca
     4681 ccagttcaat tctggtcgtg gcctccacat caaacccatt gattttacaa agatactttg
     4741 ttcgcaggcg gttccgtccg ggatccatga tccccgcccg gaactagccg ccagcatcat
     4801 ggcgcatttt ttgaaagatc ggtcgggggt ggcccgcatc gctatccgac gttgtttctg
     4861 gctggatagc caatggcttc cgtggggagg gcaagctagc cgccaaataa aaagaccgcc
     4921 cagaggcggt caggtttgga aggaagttgc cctgtcttgg caggataata gctattggca
     4981 attcccgtgc ggcgagcggg tctgctccaa ccataacgga cgttgattcg ggcgatttgg
     5041 tgtcgcgacc ggagtaattt tcgcttcgtt tcccagtaga tttatggccg tgccttgcga
     5101 gaagatagct tgcgagagga taggccgggt ttgccgccgg catgccgctt tttacctcga
     5161 acgcctgaac gcaaaacatg gcaagcttgc ttttcaatcg atcaagaacg acgctgacca
     5221 tcaacggcaa gacgcgagga tgggccgatg cccaaaaagg gaatcacggg ccgtgacgaa
     5281 tgggttgtca ccgaagcctt ggcaacggcg ctggtcgcgc tggagcagct tccaccaaag
     5341 caccagccgc gcacgcacat ggaagatatc aggaagctat tggccgccgg ttgccagccc
     5401 ggaaccgtca ccctgcatct cgcccaggcc aagtgcaggt tgtttccgga cgtggatccg
     5461 ctgaccatct atgaggaata cggcctcggc gacggacaag gctagactga cgaggccaaa
     5521 tgagaacgtg cgcagccagc aagcctcgcg caatcgatca gaaagctcgc gcggcgacga
     5581 gaaaaacctc gcgccggggc gacaaaaaaa tttcgcgccg aatcaggaga cggcgttcca
     5641 gccttccgga aacggaatca gcggccaggc cgatttgttg tcgttggcgg ccttcggcag
     5701 ctgattgaaa gtcgacgggt gggccaagaa gtcctcctcc ggcgcgggcg caatggccgt
     5761 tcgcacggca tccagcagac aatcgaactc aacttcactc atcacgctct ccgggtacgc
     5821 gagcgcacga tctcagaata cttttccgcc cagattgaaa tgatcgatca aattgtatcg
     5881 atccggcaat cggagcggac ttggttaccg attctgattc gcggttcaag agtaggccgg
     5941 cccgatccca gctaaatgtg ggcaaaatca catccggtga aaattttttg acgtatcgcc
     6001 gtgaatgaag cggcgccgca ccggcaccgt ggtacctgcg gggatacggt catgaactcg
     6061 aggtctttgc ttgcggcgac aaggggtcgc accaatgcgg ctgtggcgct cgcgctcctg
     6121 ctcgcgctat tcacaaccgg ctcggcgctg gcagcaacgg caacgccgga acagaagaaa
     6181 gcctgcacac ccgatgtcta tcgtctttgc gccggcgaaa tcccgaacgt tcgggccatt
     6241 atctcctgcc tgcggcggca gaaggcgagc ctcagcgagg cgtgccgggc ggtgttcgag
     6301 ccgtaagacg gtttcgggcg attgggcggc tggaagcggg gatcgcgtct agcgcggccc
     6361 gccaatggcg cggagcaatc ttcgcaactg tgagagatat gttggctccc cgggctggag
     6421 ccaaaaatac tatcaagata ttgtattata ttattttatt acaataatgc ccacttgata
     6481 ccccgatttg atacccgcgg tttctccgtg gagacccttc gttgagggcc ccagagccaa
     6541 gtgcggagca gccgtgaaag ggatgttcta ccagtggaac atcctaag
//
LOCUS       DATNOI010000448         3461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1271057,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000448 DATNOI010000000
VERSION     DATNOI010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271057"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VN968_17975"
     CDS             complement(<1..696)
                     /locus_tag="VN968_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HXL31157.1"
                     /translation="MKIEDVRRNAYSMPLTNPSFPPGPYRFFNREYFVITYRTDPESL
                     AAVVPEPLEVIDGTVKYEFIRMPDSTGFGDYTETGQVIPVRFNGEEGAYTHAMYLDDD
                     APIAGGRELWGFPKKRAAPKIEVESDVLVGTLHYGSVLCASGTMGYKHRCVDHDTVLQ
                     GMKAPNFILKIIPHVDGSPQICELVRFHLEDIVLKEAWTGPAALGLFPHALADVARLP
                     VREVVSALHFKADL"
     gene            complement(983..1318)
                     /locus_tag="VN968_17980"
     CDS             complement(983..1318)
                     /locus_tag="VN968_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31158.1"
                     /translation="MSPDGTKHAYWKDMLLASALVAAGFALSGLSLMQIAAHNPQMAQ
                     ATPPLQSSPAPADQDKPPAESKPGGERPTTPAPEPARPDAQAQKAGATPALPPAPAEK
                     TAAPIPDKK"
     gene            complement(1407..2426)
                     /locus_tag="VN968_17985"
     CDS             complement(1407..2426)
                     /locus_tag="VN968_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HXL31159.1"
                     /translation="MKEFDPGDNPASIVSVTDRVRPLKIGMPVTSVHFLGDNAAFVGA
                     EESVLLVSGEGAISPVAVHGGGILCAASDGERIAMGGDDGKLVALDVKGEVTLLATDP
                     KRRWIDNVALHPEGAVAWSAGKAAFIRSGKAEEKSIEVASTVGGLAFAPKGLRLAVAH
                     YNGATLWFPNMAAKPEVLEWAGSHLGVVFSPDNKFLVTTMHEPALHGWRLADNRHMRM
                     TGYPGRVRSMSWSAGGKALATSGADAIIVWPFGSKDGPMGKEPAMLAPMQARVAVVAC
                     HPKQDILAAGYSDGTILMVRMNDGAEILVRRNEAAPVAALAWNAKGTLLAFAAEDGDA
                     GLLAL"
     gene            complement(2426..3457)
                     /locus_tag="VN968_17990"
     CDS             complement(2426..3457)
                     /locus_tag="VN968_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXL31160.1"
                     /translation="MTSQKIPVTVLTGYLGAGKTTLLNRILSENHGKKYAVIVNEFGE
                     IGIDNDLIIGADEEVFEMNNGCICCTVRGDLVRILDGLMKRKGKFDAIIVETTGLADP
                     APVAQTFFVDEDVQKNARLDAVVTVADAKWLSDRLKDAPEAKNQIAFADVIVLNKTDL
                     VTKAELAEVEARIRGINPYAKLHRTERCQVALSDVLERGAFDLDRILELEPEFLDAGD
                     DHDHHHHEHDGHDHGHSHAGLKHYHDEEMQSLSLRSDKPLDPTKFMPWLQNLVASEGQ
                     KILRSKGILAFSDDDDRYVFQGVHMMLEGDHQRAWKDGEPRESRLIFIGRELPEQTIR
                     DGFERCITT"
BASE COUNT          606 a         1104 c         1093 g          658 t
ORIGIN      
        1 cagatcggcc ttgaaatgca gcgcggagac cacttcgcgc accggcagcc gcgccacgtc
       61 ggccagcgcg tgcgggaaca ggccgagcgc cgcggggccg gtccatgcct ccttcaagac
      121 gatgtcttcg agatggaagc gtaccagctc gcaaatctgc ggagagccgt cgacgtgggg
      181 aatgatcttg aggatgaaat tcggcgcctt catgccctgc agcacggtgt catgatcgac
      241 acagcgatgc ttgtagccca tggtgccgga ggcgcacagc accgagccgt aatgcagcgt
      301 ccccaccagc acgtcgctct cgacctcgat tttcggcgcg gcgcgttttt tgggaaatcc
      361 ccacagctcg cgtccgcccg cgatcggcgc gtcgtcgtcg agatacatcg cgtgggtata
      421 ggcgccctcc tcgccgttga agcggaccgg gatcacctgg ccggtttcgg tatagtcgcc
      481 gaagccggtg gaatcgggca tccggatgaa ttcgtatttg acggtcccgt caatcacttc
      541 cagcggctcc ggcaccaccg cggcgagcga ttccggatcg gttcgatagg tgatgacgaa
      601 atattcccga ttgaagaagc gatagggtcc cggcgggaat gacgggttgg tgagcggcat
      661 cgaatacgcg tttcgcctga catcctcgat cttcatttca tgcctcgtgt gctgctgagc
      721 ccggagggac tatgcggcag ccggcgataa caatcgaggc tgtaaaatcg gcatcgcaag
      781 tcaaaagatc gccagacgtc atggcgccgt cacaccgacc aacgccacgt cattccggga
      841 tgcgcctctt ggcgcaggcc cggaatccat atccccgatg gtggttatag attccgggct
      901 cgctcgtttc actcgcgccc tcagcgacaa gcgctacgcg cttgcgcgcg gaatgacgcc
      961 gagaagcgtc acaactggat aactatttct tgtccgggat cggcgcggcg gtcttctcgg
     1021 ccggagccgg cggcagcgcc ggcgtcgcgc ccgccttctg cgcttgcgcg tcgggacggg
     1081 caggctcagg cgccggcgtg gtcgggcgct cgccaccggg cttcgactcg gccggtggct
     1141 tgtcctgatc ggcaggggca ggtgatgatt gcaggggtgg ggtagcctgc gccatctgcg
     1201 ggttgtgggc tgcgatctgc attagcgaaa gaccggatag ggcaaacccc gccgcgacca
     1261 acgcggacgc gagaagcata tccttccagt acgcatgttt tgttccgtcc ggggacatct
     1321 cgttcaccgc aaaccactcg cggaatcaac ggacgccgag acccagggtt ccctcgggaa
     1381 ccggcaagac tagccgatgc cgctcgctac agcgccagca agccggcgtc gccatcttcc
     1441 gcggcgaagg cgagcagcgt gcccttcgcg ttccacgcca gggccgcaac cggggcggcc
     1501 tcattcctgc gcaccagaat ctccgcgccg tcgttcatcc gcaccattaa aatggtgccg
     1561 tcgctgtagc ccgcggcgag gatgtcctgc ttgggatgac aggcgaccac ggcgacgcgc
     1621 gcctgcatcg gcgcaagcat cgcgggctcc ttgcccatcg gaccgtcctt gctgccgaac
     1681 ggccacacga tgatcgcatc ggcgcccgag gtcgccagcg ccttgccgcc cgcgctccac
     1741 gacatcgacc tgacgcggcc gggatagccg gtcatgcgca tgtggcggtt atccgccagc
     1801 cgccagccat gcagcgccgg ctcgtgcatc gtggtgacca gaaacttgtt gtccggactg
     1861 aaaacaacgc cgagatgcga gcctgcccat tccagaactt ccggcttcgc cgccatgttg
     1921 ggaaaccaca gcgtcgcgcc attgtaatgc gcgactgcga ggcgcaggcc cttcggcgcg
     1981 aatgccagtc cgccgacggt cgaggccacc tcgatcgatt tttcctcggc cttgccgctg
     2041 cggataaagg cggccttgcc ggcggaccac gccacggccc cttccggatg cagcgcgaca
     2101 ttgtcgatcc agcgccgttt cgggtcggtc gcgagcagcg tgacctcgcc cttgacatcg
     2161 agcgcgacca gcttgccgtc gtcgccgccc atcgcgatgc gctcgccatc cgacgccgcg
     2221 cacaggatgc cgccgccatg gacggccacc ggcgatatcg cgccctcgcc gcttaccagc
     2281 aagacgcttt cctcggcgcc gacgaaagcc gcgttgtcgc cgagaaaatg caccgacgtg
     2341 accggcatgc cgatcttgag cgggcgtacc cggtcggtga ccgaaacgat ggaggccggg
     2401 ttatcgcccg gatcgaattc cttgatcacg tggtgataca gcgctcaaaa ccgtcgcgga
     2461 tggtctgctc gggcaattcg cggccgatga agatgagacg gctctcgcgc ggctcgccgt
     2521 ccttccacgc acgctggtga tcgccttcga gcatcatgtg gacgccctga aagacgtagc
     2581 ggtcgtcgtc atcgctgaag gcgaggatgc ccttcgagcg caggatcttc tggccctcgc
     2641 tggcgacgag attttgcagc cacggcatga atttggtggg gtcgagcggc ttgtccgatc
     2701 gcagcgacag cgattgcatc tcctcgtcat gataatgctt caacccggca tggctgtggc
     2761 cgtgatcgtg accatcgtgc tcgtggtggt gatgatcatg gtcatcgccg gcgtcgagaa
     2821 attccggctc gagttcgagg atgcgatcga gatcgaaggc gccgcgctcc agcacatcag
     2881 acagcgcgac ctggcagcgc tcggtgcggt gcagttttgc gtaaggattg atgccgcgaa
     2941 tccgcgcctc gacctcggca agttcggcct tggtgaccag atcggtcttg ttgagcacga
     3001 tgacgtcggc gaacgcgatc tggttcttgg cttcgggggc gtccttcagg cggtcgctca
     3061 gccatttcgc atcggcaacc gtcaccaccg catcgagcct ggcgttcttc tgtacgtcct
     3121 catcgacgaa gaaggtctgc gccaccggcg ccggatcggc aaggccggtg gtctcgacga
     3181 tgatggcgtc gaatttgccc ttgcgcttca tcaacccgtc gaggatgcgc acgagatcgc
     3241 cgcgcacggt gcagcagatg caaccgttgt tcatttcgaa aacttcttcg tcggcaccga
     3301 tgatcaggtc gttatcgatg ccgatctcgc cgaattcgtt gacgatgacg gcgtatttct
     3361 tgccgtgatt ttccgacagg atgcggttga ggagggtggt cttgccggcg ccgagatagc
     3421 cggtcagcac ggtcacggga atcttttggg acgtcaattc t
//
LOCUS       DATNOI010000449         4547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_27697,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000449 DATNOI010000000
VERSION     DATNOI010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27697"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="VN968_17995"
     CDS             <1..159
                     /locus_tag="VN968_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXL31161.1"
                     /translation="YIKRNINNAEHLSLEACFDAEAIHHSRAGETADHKEAAKAFVEK
                     RKPVFQGH"
     gene            161..925
                     /locus_tag="VN968_18000"
     CDS             161..925
                     /locus_tag="VN968_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31162.1"
                     /translation="MASYLRLRQICLVAPHLEPVISDIAGIMGLKVCYRDGNVAKYGL
                     ENALLPVDTILLEVVAPFQPGTAAERFIDKTGGRGGYMAIFCCDDPDQRGEHAKAMGV
                     RVANVIDHAPYHGVQLHPRDCRAAFIEFNHTDGSDDILGAYPPAGPDWQKSIAEDVTQ
                     ALIGVEMESPEPHALAEHWGRILGIPVSQGKAGEPELALPNCSFSFVKGVSDIMSGLT
                     FRVGDVAAVRDAAKARGYVVSNDSFLLGGVAFRLVA"
     gene            1132..3249
                     /locus_tag="VN968_18005"
     CDS             1132..3249
                     /locus_tag="VN968_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HXL31163.1"
                     /translation="MPHPLDSFFAPDSIALIGASRDLEKIPGRLLSMLRKNGFPGKLY
                     PINPNYGDIDGLKCYPSIADVGQPIDLAIVIIPARAVLSALEQCAAVGVKNAVIISSG
                     FAEEGGDSAAMQDAIVALARKTGMRVSGPNAEGFYSRVQRVAATFSPTVEVKPGAPQL
                     VATIRRIGIVAQSGGIGFAIYHRAKALGIALSYVISAGNESDLGAGEFLEYMVQDAST
                     DVILLFIEGIRDVDKFLAAAQRAAEIGKPIIVTKVGRSGAGERAAASHTASMAGWSAA
                     YDAVFAKYGFIVSNDLDEAVTTAAVLTTNPLPKGDRVAVLTVSGGAGIWGADTVSMQG
                     LQVPELSKSIQAEISALLPSYGATGNPIDVTAQGVHSGGLQRSIDLLGVSDEVDAILV
                     VLSLSSDTRMPFKQAALKPVLSAQNKPIVFYSYTLPSDFARMELAASGAVVLSGLTHV
                     GVAMRQMVARARFRLAPPADAAALPLRDISAHLKSATLSEFDSKALLRDAGIALPDEV
                     LVSRRDELDAALARVGFPLVMKIQSRDIPHKSEVGGVRVNIATKGEAFAAYQALSESA
                     RRHRPDAAIQGVLVSPMAKKGVEIIVGTLLDATFGPMIMVGLGGITTELFRDVIYRPA
                     PVSAVEATSMLAELKAAPLLNGFRGAAKTDIPALAQLISHVSVLAARARAEISEIEIN
                     PVLVHPQGQGVTVVDALAVRRRV"
     gene            complement(3385..4191)
                     /locus_tag="VN968_18010"
     CDS             complement(3385..4191)
                     /locus_tag="VN968_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXL31164.1"
                     /translation="MSKFTDIGVEKVGHVGTIEIQRPPLNFFDISLINQIADALEEFD
                     RDIEIRASVLAAQGKAFCAGANFNDPARQEQEARAAKGDPADGLGPINHLYVQAVRIF
                     RNKKPIVAAVHGAAIGGGLGLAVSADFRVTCPEARFAANFTKLGFHPGFGLTATLPEL
                     VGKNNAELIFYTSRRVTGEEATRMGLANVCVPQDQVRAEAMKLAQEIAECSPLGLLAT
                     RATMRAGLADRVMAATNHELAEQTRLRATEDFKEGIKATEERRVANFRGR"
     gene            complement(4239..>4547)
                     /locus_tag="VN968_18015"
     CDS             complement(4239..>4547)
                     /locus_tag="VN968_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL31165.1"
                     /translation="IGRLVAQLHTMRRMSVSVAGMLHAGKEPVVEASIVKDIGTIWEQ
                     QLPQRVRDLAAFVEPDASNHEILDTVLPYAMKVAPKLTIQGGTTEVLRGIIARGLGLR
                     "
BASE COUNT          828 a         1420 c         1454 g          845 t
ORIGIN      
        1 tacatcaagc gcaacatcaa caacgccgag cacctgtcgc tggaagcctg tttcgacgcc
       61 gaggcgatcc atcactcgcg cgcgggcgag accgccgatc acaaggaggc ggcaaaagcg
      121 ttcgtcgaga agcgcaagcc cgttttccag gggcattgag gtggcgagct atttgaggct
      181 gcggcagatc tgcctggtgg cgccgcatct cgaacccgtc atctccgata tcgccggcat
      241 catgggcctc aaggtctgct atcgcgacgg caatgtcgcc aaatatggcc tggagaacgc
      301 tctgctgccg gtcgatacca ttttgctgga agtggtggcg ccgtttcagc ccggcaccgc
      361 cgcggaacgc tttatcgaca agaccggcgg ccgcggcggc tatatggcga ttttctgctg
      421 cgacgatcct gatcaacgcg gcgaacatgc caaggccatg ggcgtgcgcg tcgccaatgt
      481 catcgatcac gcgccctatc acggcgtgca actgcatccg cgcgattgcc gggcggcgtt
      541 catcgaattc aaccacaccg atggcagcga cgatattttg ggggcctatc cgccggcggg
      601 tccggactgg cagaaatcga tcgctgagga cgtgacgcaa gcgctgatcg gcgtggagat
      661 ggaaagcccc gagccgcatg cgctggccga gcattggggc aggatcctcg gcattcccgt
      721 gagccaaggc aaagctggcg agccagaatt ggcgctgccc aattgcagct tcagcttcgt
      781 caagggcgta agcgacatca tgagcggcct gacgtttcgg gtcggcgatg tcgccgccgt
      841 gcgcgatgcc gccaaagcca gaggttatgt ggtgtcgaac gattcgttcc tgctcggcgg
      901 cgtcgcgttc cggctcgtgg cgtgactgcg ccaacgaata gctcgtcatg cccgggcata
      961 gccgtccgaa ggacggcgtc gcttccgctc gcctatgtcc cgggcatcca cgccttaaag
     1021 cttaaacagg aagagagacg tggatggccg ggacaagcgc ggccatgacg gaaagtgcga
     1081 catctcactg caatatgatc gcgcgctgaa tacaaaaaag gaccgtcctc aatgccgcac
     1141 ccgctcgact cgttctttgc gcccgacagc atcgccctga tcggcgcgtc gcgcgatctg
     1201 gagaaaatcc cgggccgact attgtcgatg ctgcgcaaga acgggtttcc cggaaaactc
     1261 tatccgatca atcccaatta cggcgatatc gacggcttga aatgctaccc gtcgatcgct
     1321 gatgtcgggc agccgattga tctcgccatc gtcatcattc cggcgcgcgc ggtgctcagc
     1381 gcgctcgagc aatgcgccgc cgtcggcgtc aagaacgcgg tgatcatctc gtcgggattc
     1441 gccgaggagg gcggcgacag cgccgccatg caggacgcca tcgtggcgct ggccaggaag
     1501 accggaatgc gggtttccgg ccccaatgcc gaaggattct acagccgggt ccagcgcgtc
     1561 gccgccacct tcagcccgac cgtcgaagtc aagccgggcg cgccgcagct ggtcgcgacc
     1621 atcaggcgga tcggcatcgt cgcgcaaagc ggcggcatcg gttttgcgat ctatcaccgc
     1681 gccaaggcgc tggggatcgc gctgagctac gtcatcagcg ccggcaacga atccgacctc
     1741 ggcgccggtg aatttctcga atacatggtg caggatgcct cgaccgacgt gatcctgttg
     1801 ttcatcgagg gtattcgcga cgtcgataaa tttctggccg ccgcccaacg cgccgcggaa
     1861 atcggcaagc ccatcatcgt gaccaaagtc ggccgctccg gcgccggcga gcgcgccgcg
     1921 gcatcgcata ccgccagcat ggcgggctgg tccgcggcct atgacgcggt atttgcaaag
     1981 tacggtttta tcgtttccaa cgatctcgat gaggctgtta cgacagccgc ggtgctgacc
     2041 accaacccgc tgccgaaagg cgaccgcgtc gcggtgctga cggtgtcggg cggcgccggt
     2101 atctggggcg cggacacggt ttcgatgcag ggcctgcagg tgccggaact ttccaagtcg
     2161 atccaggcgg aaatcagcgc actacttccg tcctatgggg caacaggcaa cccgatcgat
     2221 gtcaccgcgc aaggcgtgca ctccggcggc ctgcagcgga gcatcgatct gctcggcgtc
     2281 tcggacgaag tcgatgccat tctggtggtg ctgtcgctgt cgagcgacac gcggatgccg
     2341 ttcaagcagg cggcactgaa acctgtcctg tcagcgcaga acaagccgat cgtgttctac
     2401 tcctacacgc tgccgtccga tttcgcgcgg atggagcttg cggcgtcggg cgccgtggtg
     2461 ctgtcggggt tgacccatgt cggcgtcgcc atgcgtcaga tggtggcgcg cgccagattc
     2521 aggctggcgc cgccggctga tgccgccgcg ttgcctctgc gcgatatttc cgcacatctg
     2581 aaatccgcga cgctttccga attcgacagc aaggcgctgc tgcgcgatgc cggcattgcg
     2641 ttgccggacg aagtgttggt gtccaggaga gacgaactgg acgcggcact tgcccgcgtc
     2701 ggctttccgc ttgttatgaa aatccagtcg cgcgacatcc cacacaagag cgaagtcggc
     2761 ggcgtgcgcg tcaacatcgc gaccaagggc gaagccttcg cggcctacca ggcgctctcg
     2821 gaaagcgcgc gccgccatcg gccggacgct gccattcaag gcgtgctggt cagcccgatg
     2881 gcgaaaaagg gcgtcgagat catcgtcggc accctgctcg acgccacctt cggcccgatg
     2941 atcatggttg gcctcggtgg catcaccacg gaactgtttc gggacgtgat ctatcggccc
     3001 gcgccggtga gtgccgtcga agcaacatcc atgctggccg agctgaaggc agcgcccttg
     3061 ctcaatggct ttcggggcgc ggcaaaaacc gacatcccgg cattggcgca attgatttcg
     3121 catgtttccg tgctcgcggc acgagctcgt gcggagatct ccgaaatcga gatcaatccg
     3181 gtgctggtgc atccgcaagg gcagggcgtc accgtcgttg acgcgctggc ggtgcggagg
     3241 agggtatagc ttctcctcgt cattccgggc gtgagcgaag cgaacgagcc aggaatccat
     3301 aaccacgaca gggagtatgg attccgggcc tgcgccaaga ggcgcatccc ggaatgatat
     3361 gtcgaggagt gctggacaac gaactcaccg ccccctgaaa ttcgcaacgc ggcgctcttc
     3421 ggtggccttg atgccttcct tgaagtcttc ggtggcgcgc agccgggttt gttcggcgag
     3481 ttcgtgattg gtcgcggcca tcacccggtc ggcaaggccg gcgcgcatgg tggcgcgggt
     3541 tgcgagcagg ccgagcggcg aacattcggc aatctcctgc gccagcttca tggcctcggc
     3601 gcggacctga tcctgcggca cgcagacatt ggcaaggccc attctggtgg cttcctcgcc
     3661 ggtcacccgg cgactggtgt agaaaatcaa ttccgcattg tttttgccga ccagctcggg
     3721 aagcgtcgcg gtcagcccga agccgggatg aaagccgagc ttggtaaagt tggcggcgaa
     3781 ccgcgcctcg gggcaggtga cgcgaaaatc cgccgatacc gccagcccca gcccgccgcc
     3841 gatcgccgca ccatggaccg ccgcgacgat cggcttcttg ttgcggaaga tccgcaccgc
     3901 ctggacgtag aggtggttga tcgggccgag accgtccgcc gggtcgccct tggccgcccg
     3961 tgcctcctgc tcctgccgcg ccggatcgtt gaaattggcg ccggcgcaga acgccttgcc
     4021 ctgcgcggcc agcaccgacg cgcggatttc gatatcgcga tcgaattcct ccagcgcatc
     4081 ggcaatctgg ttgatcaggg agatatcgaa gaagttcagt ggcggccgct ggatttcgat
     4141 ggtgccgacg tgcccgactt tctcgacacc gatgtctgtg aatttgctca tggatagccc
     4201 tcgattgatt ttgaattaac gcagttcagg tcgcgcgcct agcgcaggcc gagcccgcgc
     4261 gcgatgatgc cgcgcagcac ctcggtggtg ccgccctgga tggtgagttt tggtgcgacc
     4321 ttcatcgcgt agggcaatac ggtgtcgagg atttcgtgat tgctggcgtc cggctcgaca
     4381 aacgctgcga gatcccgcac ccgctgcggc aattgctgtt cccagatcgt gccgatgtcc
     4441 ttgacgatgg aggcttccac cacaggttcc tttccggcgt gcagcatgcc ggcaaccgac
     4501 accgacatgc ggcgcatggt gtgaagctgc gcgaccagcc gtccgat
//
LOCUS       DATNOI010000450        13352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_333312,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000450 DATNOI010000000
VERSION     DATNOI010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13352
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333312"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VN968_18020"
     CDS             complement(<1..376)
                     /locus_tag="VN968_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3095 family protein"
                     /protein_id="HXL31166.1"
                     /translation="MTTPNDTFYSKIPVFRGFSRLMDPALYSPLPDDWSIGVADIVES
                     TRAIAEARYKAVNMAGASVIAAITNALAGREFPFVFGGDGASFAVSPEDLARAREALA
                     ATAIWVKEDLDLAMRTALVPVAA"
     gene            complement(545..1207)
                     /locus_tag="VN968_18025"
     CDS             complement(545..1207)
                     /locus_tag="VN968_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2278 family protein"
                     /protein_id="HXL31167.1"
                     /translation="MTLAYGYVKAKIVSEPVLKPSHRSHETQYHLHCNLLVEGEKWDV
                     AINVGTDDDNDLLKYKLAFDFRHPVIQTLSAAQAGSHELTGQAALPAFDFMRSDLLAE
                     TGKWRDSDVMDGSDFPEPSASLKRLLSKAHQQEFDVYVFGRFYSEGNGIHDTHLNQGS
                     TKGFIHRDGDDSNDHNDIWQDGAVMVDLGKAEWAAYFAAFNQQLVPTDDLGNPLPGAT
                     TI"
     gene            complement(1233..1778)
                     /locus_tag="VN968_18030"
     CDS             complement(1233..1778)
                     /locus_tag="VN968_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXL31168.1"
                     /translation="MTPKRIMFVRHAEKPGVPGDGKGGGVQPDGSQDGESLTVRGWQR
                     AGALARFFSSRPELKPGVIFASGLGHGSKSKRPMQTVTPLADLLNTEAVALVTTHLKD
                     DLQPLMEDVLSRQGPVLVAWEHKLIPALVERLPNPPSIPSDWPDDRFDVVWLFDRTDT
                     GWSFSQLPQLVLSGDSANPIS"
     gene            complement(1836..3494)
                     /locus_tag="VN968_18035"
     CDS             complement(1836..3494)
                     /locus_tag="VN968_18035"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HXL31169.1"
                     /translation="MRKKVQGNGLVLQAVAGTYVVTLGWDITQESVKTGLLGFAIQRA
                     DHTENETHFMRGMKTFPDTSPALPLGGTASSHDQPFQSFQWADYAAKPGHSYTYTIIP
                     MHGRPGALTDGQSLAVDVTTESEWDGTHNIFFNRGAIASQEYARRFQDKRPSVVGQAA
                     YDWLSRGLIESIITFIGKATGPGFGLRVAIYEFQWPAILDAIDAAAGRGADVKVLFDA
                     IENAQEDPVHPNEEAIATARIDGLCQGITEGKIMHNKFIVLLQRGEPVEVLTGSTNYT
                     ENGLFGHLNCAHIVNDADVAKTYLDYWTELQKNLALAELRPWNDENTPAPPNPPAPGT
                     HEVFSPQTGKATLARYGEISGTAKRALFMTFAFGMNKTFVPIYLQQDDVLRFALMDKE
                     GTGKGIEEAKATIKQIRSLGNAVVAVGQNIALNEFDRWLKEISGVQARENVRWVHTKF
                     MLVDPLSSDPIVITGSANFSDASVNTNHENMLVIRGDTRVADIYLGEFMRQFSSYAFR
                     DAAAAMGDAENFKPQDLFTDNSWIKRYEGAGTSGALRRAYFSGQ"
     gene            3756..5702
                     /locus_tag="VN968_18040"
     CDS             3756..5702
                     /locus_tag="VN968_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HXL31170.1"
                     /translation="MNKNTAMGATVRANGTGFRTWAPNARQVYVVTGGALAAHHEPSW
                     RPAPEDGLTPLGDGSWGGFLAGVGDGEPYMFFVDGIGSAGWKRDAYARELSPSPAFPD
                     STCIVRDPQSYSWRDETWQPPKFNDLIIYQLHVGTWWAQDETGRDVRKLRGGTFLDVV
                     EKLEHLQNLGVNAIQLLPIQEFETAFSMGYNGVDYFSPEGAYAVDPEQLGWRLARINR
                     MLERFGKPPLTLAELVHCTNQLKCLVDLCHLHGIAVIFDLVYNHAGGGFDDRSIWFYD
                     RQPSDNQNNSLFFTDHGWAGGQVFAYWNDWVAQFLTDNACFFLTEFRIDGIRYDEVRV
                     IENNGGRPFCQRLTHTVRATNPAAIQIAEYWNEDRASALRPPPDGLGFDAELGDRLRD
                     ALRDLLGQASAGASATLDLSDVANSLVVPAAIPEAWRLVQCLENQDLTFASHSDAARV
                     AMLADSLHRTSWYARSRSRTVTGLLLAAPGIPALFMGQELLEDKNWSDNRQVDGLIGW
                     DALDGADTARRDFLRCVADMIALRRSQPALRGSRARVSRAEDFERVIVLHRWIEGEGR
                     DVVVIANLAEQPKQAYAIGLPFAGDWFELLNSDVYDNFPNPAPVGNGGHVRAWSEPLD
                     GFAASAAVTLPANGVIVLARSSAR"
     gene            complement(5725..6519)
                     /locus_tag="VN968_18045"
     CDS             complement(5725..6519)
                     /locus_tag="VN968_18045"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXL31171.1"
                     /translation="MVAQAYALGATAVVSRPKEIIFKLAQIEIAEKAAQSDFAITSPE
                     ITQSTAAFASMFSAVRAGKQINLSDAERATLEIVNSISEKGLAAWLDDVRRYHEGTFQ
                     HCLLVTGVAVGFAIEIGFSSLDVKRLGIAATLHDIGKARIPLSVLDKPDRLDPGEEEI
                     MRQHPVIGYELLKDIPDITPEVLDGVRHHHEYLDGSGYPDGLTAPEISDLVRLLTISD
                     IFAALIESRPYRPAMPRQTAYKILRGMDGKLERSLVKAFRNVALVA"
     gene            6535..6873
                     /locus_tag="VN968_18050"
     CDS             6535..6873
                     /locus_tag="VN968_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31172.1"
                     /translation="MNDKQLFGNATQLPQHKPDLFDAIDLAQVGVDHQHAVGMDLRVA
                     RSQHLWGNVISRFQHRADGRYFRSVIGDEMNKHCRARPLKKPWPPLQSSNTNDDPPLC
                     RNLPNFLIKG"
     gene            complement(6957..8840)
                     /gene="htpG"
                     /locus_tag="VN968_18055"
     CDS             complement(6957..8840)
                     /gene="htpG"
                     /locus_tag="VN968_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HXL31173.1"
                     /translation="MTTTTDTAPISQPFQAEVAELLHLMVHSVYSETDIFLRELISNA
                     SDACDKLRYEAIAAPDLIADGEAPKILIATDEKADTLSVIDSGIGMDRQELIDNLGTI
                     ARSGTKSFLSRLTEAKDGAGLIGQFGVGFYSAFMVADRIVVTSRRAGADQVWTWSSSG
                     GSGFEIAPAGEDDAKRITRGTEIVLHLKPDAKKYLESYEIERVVGAYSDNIQFPILLK
                     SEDGEPRQINSASALWQRPKSELTPEDYKQAYKSIAGAFDEPAMTLHYRAEGRYSYAV
                     LLFAPSTKPFDLFEPSRKGKVKLYVRRVFITDDADLLPAYLRFIRGVVDSEDLPLNIS
                     REMLQNNPQLAQIRKAVTSRVITELEQLGEKEPENFAKIWDAFGPVIKEGLYEDYERR
                     EKLLALSRFTTTAGEKRSLKQYIADLKPNQTEVYFLAGDSIERLKSNPKLEQAAARGI
                     EVLLLTDPVDAFWTSAPLDFEGKPLKSLSQGDIDFSLIPRIDDSKDETTADADEAATI
                     AAIKAALGDRVADVRASQRLTSSASCLVAGSHGPDRELERLLARQNRGAGTRPILEIN
                     MRHPLVAAISGTQDAATDLSFLLLEQAQILDGELPEDPAAFANRLNQLVLRGVTKGSG
                     QAI"
     gene            complement(9282..11324)
                     /locus_tag="VN968_18060"
     CDS             complement(9282..11324)
                     /locus_tag="VN968_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HXL31174.1"
                     /translation="MFTIGIDVGGTYTDLVAIDERGKAVFAKSPSTPSDQSVGVMAGL
                     EELARRLQMTRAEMLGVTDRVVHGTTVATNALLERKGAHVALLTTEGHRDVVEMREGL
                     KGDRYDLRSPPPEPLVPRERRLGVKERLKANGKILTPLDDRSLGDAIAAIRASDATSV
                     AVCFLHSYLDPVHEIAAVERLARELPGICISRSSDVLPQIKEYERVSTTIVNAYVGPL
                     VRHYLTNLEQRLKEAGFRGSLFIVLSHGGMAPVEEASRLAAGTVLSGPAGGVSGARRC
                     ADLLGISDLVPFDMGGTSTDISLISEGRASLSADGMLAGQRIALRSLDIASIAAGGGS
                     IASVDAGGTLRVGPESAGSVPGPACYGNGGLAATVTDANVVLGYLDAAAFMGGQRPLD
                     RAASEAAVDRVAASLGLSRLEAAAGICRMINLKMADGIRLMTLRRGVDPRRFTLLSFG
                     GAAGLHAAEVARELDINRIIVPTAASVLSAWGMLTSDLRYEVSRTHHGAGPRIAAEEA
                     RALFCQLEQRAAGRLRAWFDGPMSIERSAEMRYGEQIFEIDVALDDLDWNAASLVDQI
                     EDRFHRRHEELYTYSSRDQEVVFVNARVAAVGEVSRRGQDERPAPSAAACAPRTTRQA
                     FFGAWREVPVFALDTLEPGHSFEGPAVVEAETTTVIVGAGDRVTVNAVGWLDIALG"
     gene            complement(11324..12994)
                     /locus_tag="VN968_18065"
     CDS             complement(11324..12994)
                     /locus_tag="VN968_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HXL31175.1"
                     /translation="MSPKIDPITRSVVQHRLSSIVKEMGEAMLRTSYSQILNSSRDFS
                     LGICDTQGRLIAQADHIPVHVGALPWAALAVEARFKEVRPGDVILLNDPYHGGSHLPD
                     LTAFVPIFDGERRLLWTIVRAHQSDIGGATHGGYNPGATEIFQEGLRVPPIKLYEAGL
                     LRDDLLDLLALNIRNPREFRGDLAAMVGAAHLGERRLSRLFAEFGAPVVEAAVETILD
                     AAEQQTRAVVSTWKDGVFYGEAFLDDDGHGRSDIRIAAKVTKSGSDIEVDLSDSDPQS
                     TSFVNSSHANMQAAVVMAFAYLIDADIPKNAGALRPLKVIARQGTIVWADPGRPVTLC
                     TSHPSNEIVEAIVKAMSASCPDRAMAGWGRRFRIAIQGEDPRSGRNFIWHLFQARPGG
                     GASSGGDGWSSIGEWHSVGGIKFGSIEVAEVRFPLHFRHHEFRPGSGGDGQFRGGFGV
                     ALDLVLETAKPARANTAGDGARHGPCGMLGGEDGVPHHYRLISEGRAPRVLRTKEVGI
                     EILPNDCLEVRSSGGGGWGPPARRSPAARQRDREQGLAQDSATGQGGR"
     gene            complement(13040..>13352)
                     /locus_tag="VN968_18070"
     CDS             complement(13040..>13352)
                     /locus_tag="VN968_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXL31176.1"
                     /translation="IGAAVRAGINEAFRRHGVPGGTVGLGSLLKIHFADRPIRDYRSA
                     YMTEQELLRQAIFNRGLLNRGVLAAGYGLMALSTPMTDADIDTVITAASDALKEVSAS
                     A"
BASE COUNT         2503 a         4132 c         4159 g         2558 t
ORIGIN      
        1 cggctgcgac cggcaccagc gccgtccgca tcgcgaggtc gaggtcttcc ttcacccaga
       61 tcgcggtcgc cgccagcgcc tcgcgcgccc gcgcgagatc ctcgggcgac accgcaaagc
      121 ttgcgccgtc gccgccaaac acgaacggaa actcacgccc cgcaagtgcg ttggtgatcg
      181 cggcgatcac ggaggcgccg gccatgttga ccgccttgta acgcgcttcc gcgatcgccc
      241 gggtcgattc cacgatatcg gcgacgccga tgctccagtc atccggcaac ggcgaataca
      301 gcgccggatc catcaggcgg gaaaagccgc gaaagaccgg gatcttgctg tagaacgtgt
      361 cgttgggcgt ggtcatgggc gcgtgaccgc cggatgtaag atcgtctgac agatacggcg
      421 gggctcggta ttcgggttca gagcaagtgc atggtgcggc gtgccacgag cttatatgaa
      481 gaaaaaagcc tcgaagacaa attgttttca gagacgttgt ctagccggtt actgcccgtg
      541 caccctaaat cgtcgtcgcg ccgggcaacg ggttgccgag atcgtcggtc ggaacgagct
      601 gctggttgaa cgcagcgaag tatgccgccc attcagcctt gccgaggtca accatgacgg
      661 ccccgtcctg ccagatatcg ttgtgatcgt tggagtcgtc gccatcgcga tggatgaagc
      721 ccttggtcga tccctgattc aaatgggtgt cgtgtatccc attgccttcg ctataaaaac
      781 gaccgaagac gtagacgtcg aattcctgct ggtgtgcttt cgacagcagg cgtttgagcg
      841 atgcggaagg ttcgggaaag tcggacccat ccatcacgtc gctgtcgcgc cacttgccgg
      901 tttcggcgag caagtcgctg cgcatgaagt cgaacgcggg cagggcagcc tgtccggtca
      961 gttcatggga accagcttgt gcggccgaga gcgtctggat gaccggatgc ctgaaatcga
     1021 aggcgagttt gtatttcagc agatcgttgt catcgtcggt tccgacgttg atcgcaacgt
     1081 cccacttctc gccctccacc agaaggttgc agtggagatg gtactgggtt tcgtgcgaac
     1141 ggtgcgatgg ctttaaaacc ggctccgaga cgattttggc ttttacatat ccgtaggcga
     1201 gcgtcaccgt tgaatccctt ccggtttgaa cttcaggaga ttggattggc gctgtcaccc
     1261 gacagcacga gctgcggcag ctgggaaaaa gaccagcccg tatcggtgcg gtcgaacagc
     1321 caaaccacgt cgaagcggtc gtcgggccag tcgctgggaa tgctgggcgg attcggcagc
     1381 cgctccacga gcgccgggat tagtttatgc tcccacgcca ccagcaccgg gccttggcgg
     1441 gacaagacat cctccatcag gggttggaga tcgtccttca gatgagttgt gaccaaggca
     1501 accgcttcgg tgttgagcag gtccgcaagc ggcgtaaccg tctgcatcgg ccgcttgctc
     1561 ttgctgccgt gcccaaggcc agaggcgaaa atgacgcccg gcttcagttc cgggcgcgaa
     1621 ctgaagaacc gggccagggc gccggcgcgt tgccagcccc ggaccgtcag gctttcgccg
     1681 tcctgcgaac cgtccggttg gacgcccccg cccttgccgt cgccgggaac gccgggcttt
     1741 tcggcatggc gaacaaacat gatgcgcttc ggcgtcatcg cattctccgc acggcactag
     1801 aaaacagacg cagtcgcttc tcctgcatgg aacagctact ggccggaaaa atacgcccgg
     1861 cgcagcgcgc cggacgtgcc ggcgccttca tagcgcttga tccaagaatt gtccgtgaac
     1921 agatcctgtg gcttgaagtt ctctgcatct cccatggctg cggccgcgtc gcgaaaggca
     1981 tagcttgaga actgccgcat gaattcgccg agatagatat ccgcgacccg ggtgtcgccg
     2041 cggatgacca gcatgttctc atggttggtg ttcacgctgg cgtcgctgaa gttcgccgaa
     2101 ccggtgatca cgatgggatc gctggaaagc gggtcgacaa gcatgaattt ggtatgaacc
     2161 catcggacat tttccctcgc ctggacgccg ctgatctcct tcagccagcg atcgaactcg
     2221 ttcaaggcga tattctggcc gaccgcgacc acggcattcc ccaggcttcg gatttgcttg
     2281 attgtcgcct tggcctcctc gatgcccttg ccggtacctt ccttgtccat gagggcaaac
     2341 cgcagcacgt cgtcctgctg gaggtagatc ggcacgaagg ttttgttcat cccgaacgcg
     2401 aatgtcatga agagcgcgcg tttcgccgtg ccggaaattt cgccataccg cgcaagtgtt
     2461 gccttgccgg tttgcggcga gaagacttca tgcgttcccg gggctggcgg gttcggcggc
     2521 gcgggggtgt tttcgtcatt ccatggtcga agctcagcaa gcgccaggtt tttttgcagc
     2581 tctgtccagt agtcgaggta tgttttcgcg acatcagcgt cattgacgat gtgcgcgcaa
     2641 ttgagatggc cgaacagtcc attctccgtg taattggtcg atcccgtgag cacctcgacc
     2701 ggctcgcccc gctgcaacag gacgatgaac ttgttatgca tgatcttgcc ttcagtaatg
     2761 ccctggcaga gaccatcgat cctggcggta gcgatcgctt cctcgttggg gtgaaccgga
     2821 tcctcctgcg cattctcgat ggcgtcgaac aagaccttga cgtcggctcc tcgcccggcg
     2881 gcggcatcaa tggcgtcaag aatggcgggc cactgaaatt catagatcgc cacccgcaag
     2941 ccgaagcccg gccctgttgc cttcccgata aaggtaatga tggactcgat caggccgcgc
     3001 gacagccagt cataagcggc ctggccgacc acgctcggcc ttttgtcctg aaagcggcgc
     3061 gcatattcct gcgacgcgat cgcgccgcga ttgaaaaata tgttatgggt gccgtcccat
     3121 tcggattcag tcgtgacgtc gacggcgagc gattgaccgt cggtgagcgc gcccggacgg
     3181 ccgtgcatgg gaatgatggt gtaggtatag gaatgtccgg gcttggcggc gtagtcggcc
     3241 cactgaaaac tctggaacgg ctggtcgtgg ctcgaggcgg tgccgcccag cggcaaggcg
     3301 ggcgatgtgt ccggaaaagt cttcatgccg cgcatgaagt gggtctcatt ttcggtatgg
     3361 tcggccctct ggatggcaaa gccgagcagg ccggttttga cgctttcctg agtgatgtcc
     3421 cagcccagcg tgacgacata ggtgccggca acagcttgca gcacaaggcc gtttccttga
     3481 acctttttcc tcatgacggc actccaaaaa aatgattttt caaaaaaccg atcgtccgcg
     3541 cttgataagt taaacaaggc aatcgaattc tgtatgacga gaatctgtat gacgaaatgc
     3601 gtcggcccgg cacgatcgaa gcccgtgata cccgtggggt tggcgcaccg ggaatttcga
     3661 ttttggccgg cagcctatgc taggtgtaca acaagaacat tacaaccttt cgaaaagaaa
     3721 ggatttggac cgatatgccg gtcgatttga gccgagtgaa caagaacacc gccatggggg
     3781 ccacggtgcg ggccaatggg accggctttc gcacctgggc gccgaatgca aggcaggttt
     3841 acgtggtgac cggcggcgcg cttgcagcgc accacgaacc gtcctggcgc ccggctccgg
     3901 aagatggttt gacgccgctc ggtgacggaa gctggggcgg ctttctggcc ggagtgggcg
     3961 acggcgagcc ttacatgttt ttcgtcgatg ggatcggcag cgccggatgg aagcgggatg
     4021 cctatgcgcg cgagctcagc ccgtcgcccg ccttccccga cagcacctgc atcgttcgcg
     4081 atccgcaaag ctattcctgg cgcgacgaaa cctggcagcc gcccaaattc aacgatctca
     4141 tcatctatca gcttcacgtc ggcacctggt gggcgcagga cgagaccggc cgcgatgtcc
     4201 gcaagctgcg cggcggcacg ttcctggacg tcgtcgaaaa acttgaacat ttgcagaatc
     4261 tcggcgtcaa cgccattcaa ctgctgccga ttcaggaatt cgagacggcc ttcagcatgg
     4321 gctacaatgg cgtcgactat ttttcgcccg aaggcgcata tgccgttgac cccgagcaac
     4381 tcggctggcg gcttgcgcgt atcaaccgga tgctcgaacg cttcggcaag ccgcctctga
     4441 cgcttgccga acttgtccac tgcaccaatc agttgaaatg tcttgtcgac ctctgccatc
     4501 tgcatggcat cgccgtgatt ttcgatctgg tctataacca cgccggcggc ggtttcgacg
     4561 atcgcagcat ctggttttac gaccggcagc caagcgacaa ccagaacaac agtcttttct
     4621 tcacggatca tggctgggcc ggcggtcagg tcttcgccta ttggaatgat tgggtagcgc
     4681 agttcctgac cgacaacgcc tgcttcttct tgaccgaatt tcggatcgat ggaattcgct
     4741 acgacgaggt gcgggtgatc gagaacaatg gcggccgccc gttctgccag cgcctgacgc
     4801 acacggtgcg ggcgaccaat cccgcggcga tccagatcgc cgaatactgg aacgaggatc
     4861 gcgccagcgc gctgcgcccg cctcccgacg ggcttggctt cgacgcggag cttggcgacc
     4921 gtctgcgcga tgcgctgcgc gacctgctcg gacaagcatc cgccggtgcc tcggcgacgc
     4981 tggatctttc agacgttgcg aactcccttg ttgtgcccgc cgcgattccc gaggcctggc
     5041 ggctggtgca atgcctcgaa aatcaggacc tgacgtttgc gtcgcattcg gatgcagccc
     5101 gggtcgccat gctggcggac tctttgcacc gaacctcctg gtacgcccgc agccgaagcc
     5161 ggacggtcac cggcctgctg ctggccgcgc cgggcattcc cgccttgttc atgggtcagg
     5221 aattgctcga ggacaagaac tggagcgaca accggcaggt cgatggcctc atcggatggg
     5281 atgcgctgga tggcgctgat accgcaaggc gcgactttct aagatgcgtc gccgacatga
     5341 tcgcgcttcg acgttcgcag ccggcactgc gtggtagccg cgcacgggtg tcgcgcgccg
     5401 aagatttcga acgggtgatc gttttgcacc gatggatcga gggagaaggc cgggatgtgg
     5461 tcgtgatagc caatctggcc gagcaaccga aacaggctta tgcgatcggg ctgccgtttg
     5521 ccggggactg gttcgaactg ctcaacagcg atgtctatga caactttcca aatcccgcgc
     5581 cggtcggcaa tggcggccac gtgcgtgcct ggagcgagcc gctggatggt tttgcggcaa
     5641 gcgccgccgt cacgcttcca gccaatggcg tgatcgtgct ggcgcgttca agcgcgcgtt
     5701 aagcatccat tgagcgcaag cgcatcacgc cacaagcgca acgttccgga acgccttcac
     5761 cagcgatcgc tccagctttc cgtccatccc acgcaggatc ttgtaggcgg tttggcgggg
     5821 cattgccggt cggtacggcc tcgactcaat cagggctgcg aaaatatcgg agatcgtcag
     5881 caatcttacc agatcggaaa tctccggcgc cgtcagcccg tcgggataac cggagccgtc
     5941 cagatattcg tggtggtgtc ttacgccatc aagaacctcg ggggtgatgt ctgggatatc
     6001 cttcaataat tcatatccga tcaccgggtg ctgtcgcatg atctcttctt cgcctggatc
     6061 aaggcgatcg ggcttgtcga gaaccgacaa gggaatgcgg gctttgccga tgtcgtgaag
     6121 ggttgccgcg attcccagtc gctttacatc cagactggag aacccgatct ctatcgcaaa
     6181 gccgactgcc actcccgtca ccagcagaca atgctggaag gtcccttcgt gatagcggcg
     6241 tacgtcgtca agccacgcgg caagcccttt ttccgagatg ctgttgacga tttccaaagt
     6301 ggcgcgctcg gcatccgaca gatttatttg tttgccggcc cgtaccgccg aaaacatcga
     6361 cgcgaaggcc gccgtgctct gtgtgatctc gggtgaggta atggcaaagt cgctttgtgc
     6421 cgccttctcg gcgatttcga tctgcgccag cttgaagatg atttctttgg gacgcgaaac
     6481 gaccgcggtc gccccgagcg cataggcctg cgcgaccatg gagcgaacct ggtgatgaac
     6541 gacaaacagc ttttcggaaa tgcaactcag ctcccgcaac acaagcctga tctgttcgac
     6601 gcgatcgatc ttgcgcaggt cggcgtcgac catcagcacg ccgttggaat ggacttgcgc
     6661 gtcgcccgtt cccaacattt gtggggcaac gtcatatcgc ggttccagca tcgcgcggat
     6721 ggccggtatt ttcgcagcgt catcggtgac gaaatgaaca agcattgcag agccagaccc
     6781 ttaaaaaagc cgtggccgcc acttcaaagc agtaatacta acgatgaccc accattgtgc
     6841 cggaacctgc ctaacttcct gatcaagggc taacgacata ttacgattgt cggatcagcg
     6901 gtatcctgtg ccgggtttgc cgatctcggt tgcgccaatt tgaactcacc ctctggctat
     6961 atcgcctggc cgctcccctt cgtcaccccg cgcagcacca gctggttgag ccggttggca
     7021 aaggcggcgg gatcttccgg cagctcaccg tcgagaattt gcgcctgctc cagcagcaga
     7081 aacgacaaat ccgtggcggc gtcctgggtc ccggaaatgg ccgcgaccaa gggatggcgc
     7141 atgttgattt cgagaatcgg cctggtgccg gcgcctcggt tctgccgtgc cagcaatcgc
     7201 tcgagctcgc ggtccgggcc gtggctgccg gcgaccaggc acgacgcgct tgacgtcagc
     7261 cgttgcgagg cgcgcacgtc ggcgacgcgg tcgccgaggg cggccttgat cgcggcgatg
     7321 gtcgcggcct cgtcggcgtc cgctgtggtt tcgtccttgc tgtcgtcgat gcgcgggatc
     7381 aggctaaagt cgatgtcgcc ctggctcagc gatttgagcg gcttgccttc aaaatcgagc
     7441 ggcgccgagg tccagaacgc gtcgaccggg tcggtcagca acaagacttc gatgccgcgg
     7501 gcagccgcct gctccagctt gggattggat ttcagccgct cgatgctgtc gccggccaga
     7561 aagtagacct cggtctgatt gggcttcagg tccgcgatat attgcttcag cgagcgcttc
     7621 tcgcccgcgg tggtggtgaa gcgcgacagc gccagcaatt tctcgcgccg ctcatagtcc
     7681 tcatagaggc cttccttgat cacggggccg aaggcgtccc agatttttgc aaagttttcc
     7741 ggctccttct cgccaagctg ctcgagctcg gtgatcaccc ggctggtcac cgcttttctg
     7801 atctgcgcca gctgcggatt gttctgcagc atttcgcgcg agatgttgag cggcaggtcc
     7861 tcgctgtcga cgacgccgcg gatgaagcgc aaataggccg gcagcagatc ggcatcgtcg
     7921 gtaatgaata cccggcggac atagagcttc accttgccct tgcgcgacgg ctcgaacaga
     7981 tcgaacggtt ttgtcgatgg cgcgaacaac agcaccgcgt aggaatagcg gccttcggcg
     8041 cggtaatgca gcgtcatcgc cggttcatcg aaggcgccgg cgatcgactt gtaggcctgc
     8101 ttgtaatcct ccggcgtcag ttcggatttc ggccgctgcc acagcgcgct cgccgaattg
     8161 atctggcgcg gctcgccgtc ctccgattta agcagaatcg gaaactggat attgtcggaa
     8221 taggcgccga cgacacgctc gatctcgtaa ctctcgagat atttcttcgc gtcgggtttt
     8281 aggtggagca cgatctcggt tccgcgcgtg atacgctttg cgtcgtcctc gccggcgggc
     8341 gcgatttcaa agccgctgcc gcccgatgac gaccaggtcc agacctgatc cgctccggcg
     8401 cggcggctgg tgacgacgat gcggtcggcg accatgaagg cggaataaaa cccgaccccg
     8461 aactggccga tcaggccggc gccatccttg gcctcggtca ggcgcgacag aaacgatttg
     8521 gtgccggagc gcgcgatggt gccaagattg tcgatcagct cctgccggtc catcccgatg
     8581 ccgctgtcga tcacgctgag cgtatcggcc ttctcgtcgg tcgcgatcag gattttgggc
     8641 gcctcaccat cggcgatcag atcgggcgcc gcgatggcct cgtagcgcag cttgtcgcag
     8701 gcatccgagg cattggaaat caattcgcgc aggaaaatat cggtttccga atagaccgaa
     8761 tgcaccatca ggtgcaggag ttcggccacc tcggcctgga acggctggga tatgggggcg
     8821 gtgtcggtag tggtggtcat tatcggtctc gctgggtgac ggggcagaaa cggacgaacg
     8881 gaaatcggga aatgtccgat atagcgatgc cattgcgggt ggcaagcttc atggtgctac
     8941 cacggaaaac gcagaaagcc gccacgaggg ctgccttcga agaacggcgg cggaaaccgg
     9001 cccctggtcg ggatcggaac cgctttagcc gcttgtgaaa cgcgatggtt ttttcggcaa
     9061 tctcctgcag gcagtcctct accgcgaagt gcggtgaccg gaacggcgcc cagatctgct
     9121 gctttgtacg cggtcgtcga cggtcccgtc cgagccatgg tcctcagcgg cacgatcgcg
     9181 aaatccgaga aagacttgcg cgagcggatc accggcgcag tcggactctt gctggatgga
     9241 tgccgcgccc ctgctgcgcg gcgcggctag ttcggttggc atcaaccgag cgcaatatcc
     9301 agccaaccca ccgcgttcac ggtcacccgg tcgccggcgc cgacgatgac cgtcgtggtc
     9361 tcggcttcga cgacagccgg cccttcgaaa gagtggcccg gttcaagggt atcaagcgca
     9421 aaaaccggca cctcgcgcca cgcgccgaaa aacgcctgtc gcgtcgtgcg cggcgcgcag
     9481 gcggcggccg atggcgctgg ccgctcatct tgtccgcgcc gtgacacttc gccgactgcc
     9541 gcgacgcgcg cgttgacgaa cacgacttcc tgatcgcgcg aggaataggt gtaaagctcc
     9601 tcgtgacggc gatgaaagcg gtcttcgatt tgatcgacca gcgaggccgc attccagtcg
     9661 agatcatcga gcgcaacgtc gatctcgaag atctgctcgc cgtagcgcat ttcggcggag
     9721 cgttcgatgc tcatgggccc gtcgaaccag gcgcgaagcc ggccggcggc ccgttgctcc
     9781 agttgacaaa acagcgcgcg cgcctcctcc gccgcaatgc gcgggccggc gccgtgatgg
     9841 gtgcggctga cttcgtagcg caggtcgctg gtcagcatgc cccatgccga cagcaccgaa
     9901 gccgccgtcg gcacgatgat gcggttgata tcgagttcgc gcgcgacttc ggcggcatgc
     9961 aggccggccg cgccgccaaa actcaacagc gtaaaccgcc tgggatcgac accgcggcgc
    10021 aacgtcatca gccggatgcc gtccgccatc ttcagattga tcatgcggca gatgccagcg
    10081 gcggcttcga gccgcgacag gccaagagac gccgcgaccc ggtcgaccgc cgcttccgac
    10141 gcagcgcgat cgagcgggcg ctgcccgccc atgaaggcgg cggcgtcgag ataaccgaga
    10201 acgacattgg catcagtgac ggtcgcggca aggccgccat tgccatagca ggccggcccc
    10261 ggcaccgagc ccgcgctctc gggcccgacg cgcaacgtgc cgccggcatc gacgctggcg
    10321 atcgagccgc cgccggccgc gatgctggca atgtcgagac tgcgcagggc gatgcgctgc
    10381 ccggcgagca ttccgtccgc cgatagtgac gcgcggcctt cagaaatcaa cgagatgtcg
    10441 gtgctggtgc cgcccatatc gaacggcacc agatcggaaa tgccgagcag atcggcacag
    10501 cgccgcgcgc ccgaaacgcc gccggcggga cccgacagca ccgtgcccgc ggcaagccgc
    10561 gaggcttctt ccaccggcgc catgccgcca tgcgacagca cgatgaagag actgcccctg
    10621 aagcccgcct ccttgagacg ctgctccaga ttggtcagat agtgccgcac cagcggcccg
    10681 acgtaggcgt tcacgatcgt ggtcgagacc cgctcatatt ccttgatctg cggcaacaca
    10741 tcgctcgacc tggaaatgca gataccggga agttcgcgcg cgaggcgttc gaccgcggcg
    10801 atttcgtgga ccgggtcgag ataggaatgc agaaaacaca ccgcgaccga tgtggcgtct
    10861 gacgcgcgga tcgctgcgat ggcatcgccg agcgagcggt cgtcgagcgg cgtcaagatc
    10921 ttgccattgg ccttgagccg ttccttcacg ccaaggcgcc gctcgcgcgg caccagcggc
    10981 tccggcggcg gcgaacgcag atcgtagcgg tcgcctttga gaccctcgcg catctcaacg
    11041 acatcgcgat ggccttcggt cgtgagcagc gcgacatgag cgcccttgcg ctccagcaag
    11101 gcgttggtcg cgaccgtggt tccgtgaacc acccggtccg tcacgccaag catttccgcg
    11161 cgcgtcattt gcaatcgccg cgccaactct tccagcccgg ccatgacgcc gaccgactga
    11221 tctgatggcg tcgacggcga tttcgcgaac acggctttgc cacgctcgtc gatcgcaacc
    11281 agatcggtgt aggtcccgcc gacgtcaatt ccgatcgtga acatcaccgg ccgccctgcc
    11341 ccgtcgcgct gtcttgcgcc agtccctgtt cgcggtcgcg ttgacgcgcg gccggtgatc
    11401 gccgcgccgg cggtccccag ccgccgccgc cggaggagcg cacttcgagg caatcgttgg
    11461 gaagaatttc gatgccgacc tccttggtgc gcaacacgcg cggcgcgcgg ccctcggaga
    11521 tcaggcggta atgatgcggc acgccgtctt cgccgccgag cattccgcag ggcccgtgac
    11581 gcgcgccgtc gcccgcggtg ttggcacgcg cgggttttgc cgtttccagc accagatcga
    11641 gcgcgacacc aaaaccgccg cgaaactgtc cgtctccgcc ggagcccggg cgaaattcat
    11701 ggtgacggaa atgcagcgga aaccgcacct cggcgacttc gatgctgccg aacttgatgc
    11761 cgccgacgct gtgccattcg ccgatcgacg accagccgtc gccgccggag gaagcgccgc
    11821 cgccggggcg ggcctgaaac aagtgccaga tgaaattgcg tcccgaacgc ggatcttctc
    11881 cctggatcgc gatccggaag cggcggcccc atcccgccat ggcacggtcc ggacatgagg
    11941 ccgacatcgc cttgacgatg gcctcgacga tctcgttcga gggatggctg gtgcagagcg
    12001 tcaccggccg gcccggatcg gcccagacaa tggtgccttg tctggcgatc accttgagcg
    12061 gccgcagcgc gccggcattt ttgggaatat cggcgtcgat cagatacgca aaggccatca
    12121 ccacggcggc ctgcatgttg gcatgcgagg aattcacaaa actggtcgac tgtggatcgg
    12181 agtccgagag atcaacctcg atatcgctgc ccgatttcgt gaccttggcg gcgatcctga
    12241 tgtcgctgcg accatgaccg tcgtcgtcga ggaacgcttc gccatagaac acgccgtcct
    12301 tccaggtcga aaccaccgcc cgcgtctgtt gctcggccgc atccagaatg gtctcgaccg
    12361 cggcctcgac caccggcgcg ccgaattcgg cgaatagccg cgacagccgg cgctcgccga
    12421 gatgcgcagc accgaccatt gccgcgagat cgccgcgaaa ctcgcgtggg ttgcgaatgt
    12481 tcagcgccag caagtcgagc agatcgtcgc gcagcaggcc ggcttcgtac agcttgatcg
    12541 ggggaacgcg cagcccctcc tgaaaaatct cggtggcgcc gggattgtag ccgccatggg
    12601 tggcgccgcc gatgtcactc tggtgggcgc gcacgatggt ccacagcaac cggcgctcgc
    12661 catcgaagat cggcacgaac gcggtcaggt caggcagatg gctgccacca tgatagggat
    12721 cgttgagcag aataacgtcg ccgggccgga cctccttgaa ccgcgcctcg acggcgagcg
    12781 ccgcccaggg tagcgcgccg acatgcaccg ggatgtgatc ggcctgcgcg atcaggcggc
    12841 cctgtgtgtc gcaaatgccg agcgagaaat cgcggctgga attgaggatt tgcgaatagg
    12901 aggtgcgaag catggcttcg cccatttcct tcacgatcga gctgagacga tgctgaacga
    12961 cggatcgcgt gatgggatcg atttttgggg acatgcgctc ctgctggcaa aaaaattgcg
    13021 tcggctgacc gaggggtcgt caggctgagg cggacacctc tttcagcgcg tcggacgccg
    13081 ccgtgatgac cgtatcgata tcggcatcgg tcatcggcgt cgagagcgcc atcaacccgt
    13141 agcccgcggc cagcacgcct cgattgagca atccccgatt gaagatcgcc tgacgcagca
    13201 gttcctgttc ggtcatgtat gccgaccgat agtcacggat cggccgatcg gcaaaatgga
    13261 tttttagcag cgaacccagc ccgaccgtgc cgcccggcac accatgacgg cggaaggctt
    13321 cgttgatgcc ggcgcgcacc gccgcgccga tc
//
LOCUS       DATNOI010000451         2161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1211616,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000451 DATNOI010000000
VERSION     DATNOI010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211616"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VN968_18075"
     CDS             <1..383
                     /locus_tag="VN968_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HXL31177.1"
                     /translation="RVQDTLADLAGIMGARDAAICREMTKMHEEVRRAPVSELARAVD
                     TQETRGEFVLVIGPPAAGTQIMTEPELDQLLRSSLMRDSVKDAVAHAVELSGRPRREI
                     YARALELAKETGKRAGAEGEDGKD"
     gene            370..783
                     /locus_tag="VN968_18080"
     CDS             370..783
                     /locus_tag="VN968_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXL31178.1"
                     /translation="MAKTDDAQPVATRVKTAAPARVAAFRTGLSAEARATAYLMAKGY
                     RILAKRFRTPYGEIDIVARRRNLLAFVEVKARASLDEAAYAVTPRQQRRIIDAAQAWL
                     MAHPEHANFDLRFDAILIAPRRLPRHLLAAFDAST"
     gene            824..1765
                     /gene="gshB"
                     /locus_tag="VN968_18085"
     CDS             824..1765
                     /gene="gshB"
                     /locus_tag="VN968_18085"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HXL31179.1"
                     /translation="MKLNVAVQMDPIERINIRGDSTFALLLEAQKRGHGLSYYTPDKL
                     SLRGEELVAPVQLLTVRDEPGDHFTLGEARREPLEAFDVILLRQDPPFDLAYITSTHF
                     LERIYPKTLVVNNPASVRNAPEKIFVMDFPHLMPPTLISRDLDEINSFRDEHGAVVMK
                     PLHGHGGAAVFRIMPQDMNFGSLFDMFTVTFREPWVIQRFLPEVKDGDKRIILVDGEF
                     AGAVNRVPAPDDLRSNMVRGGAAQATELSPREREICAALGPGLRERGLLFVGIDVIDG
                     HLTEINVTSPTGIRAIARLGGPDVAAKIWDAIEAKRG"
BASE COUNT          410 a          699 c          674 g          378 t
ORIGIN      
        1 accgcgtgca ggacacgctg gccgatctcg ccggcatcat gggcgcgcgc gacgccgcga
       61 tctgccgcga aatgacgaaa atgcacgaag aggtcaggcg cgcgccggtg tccgaactgg
      121 cgcgggccgt ggacacgcag gagacgcgcg gcgaatttgt gctggtgatc ggcccgcccg
      181 cggcgggcac gcagatcatg acagagcctg agctggacca actgttgcgg tcatcgctga
      241 tgcgcgacag cgtcaaggac gcggtcgcgc atgcggtcga actgtccggc cggccgcgcc
      301 gcgagatcta cgctcgcgcg cttgaactcg ccaaggagac cggcaagcga gccggggcgg
      361 agggcgaaga tggcaaagac tgacgacgcc caacccgttg caacgcgcgt aaaaaccgcc
      421 gcgccggcgc gggtcgcggc gtttcgaacc gggctctccg cggaagcccg cgccaccgct
      481 tatctgatgg caaagggcta tcgcatcctc gccaaacgtt ttcgcacacc ctatggcgag
      541 atcgacatcg tggcgcgccg gcgcaatctg ctggcctttg tcgaggtcaa ggcccgcgcc
      601 agcctggatg aggccgccta tgcagtgacg ccgcgccagc agcggcgcat tatcgatgcc
      661 gcgcaggcct ggctgatggc gcatcccgaa cacgccaatt tcgatctgcg ttttgacgct
      721 atcctgattg cgccgcggcg gttgccgcgc catctgttag cggcgttcga cgccagcacc
      781 tgaacactta accttccaac aaaaccaact tgcgggccag cccatgaaat tgaacgtcgc
      841 cgtccagatg gaccccatcg agcgcatcaa catccgcggg gattcgacct tcgcgctgct
      901 gctggaggcg cagaaacgcg gccacggcct gtcctattac acgccggaca aactctcgct
      961 gcgcggcgag gaactggtcg ccccggttca attgctcacc gtgcgcgatg agcccggcga
     1021 tcatttcacg ctcggtgagg caaggcgcga gccgcttgag gcattcgacg tgattttgct
     1081 gcggcaggac ccgccattcg atctcgccta catcacctcg acgcattttc tggagcggat
     1141 ttatcccaag acgctggtgg tcaacaatcc cgccagcgtg cgcaacgcgc cggaaaagat
     1201 cttcgtgatg gattttccgc atttgatgcc gccgaccctg atctccaggg atctcgacga
     1261 gatcaactcg ttccgcgacg agcatggcgc cgtggtgatg aaaccgctgc acggccacgg
     1321 cggcgcggcg gtgtttcgca tcatgccgca ggacatgaat ttcggttcgc tgttcgacat
     1381 gtttacggtg acattccgcg agccatgggt gatccagcgc tttcttcccg aggtcaaaga
     1441 cggcgacaag cgtatcatcc tggtcgacgg cgaattcgcc ggcgcggtca accgggtgcc
     1501 ggcgccggac gatctgcgct ccaacatggt gcgcggcggc gcagcgcaag ccaccgagct
     1561 ttcgccgcgc gagcgtgaaa tctgcgcggc attgggtcct ggcttgcgcg agcgcggatt
     1621 gttgttcgtc ggcatcgacg tcatcgacgg ccacctcacc gagatcaacg taacctcgcc
     1681 gaccggcatc cgcgccatcg cgcggctcgg cgggcctgat gtggccgcga agatctggga
     1741 tgcgatcgag gcgaagcggg ggtgagcgcc cgaccaaaat gcagcgcgtc cgggacgcga
     1801 gtggaatttg ctctcgccat cgtgcggtga tttgcccgtc aggcaatttg tatcgagtcc
     1861 cgcttgcaaa aatatttcgg tttccacgca ccccacccca ccagaggggc gtatcgcgat
     1921 cgtcacggac gcggggaggg atgcggtgga cgcagcagcg ttttgctgcg cgacgtgtag
     1981 cgcgcaaaat caaatgacac cgcgcgcaac ttcagctgtc cccctgtttt ggatgccttc
     2041 aaccggcaaa aaccggacaa ccggcatttc cttatcccct aaccggacat gacccggaca
     2101 tgccaaaatc gacgcgattg acccataccg cacatcacgc ctgcgtactg gcagcccgtg
     2161 g
//
LOCUS       DATNOI010000452         7756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_913684,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000452 DATNOI010000000
VERSION     DATNOI010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913684"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1106)
                     /locus_tag="VN968_18090"
     CDS             complement(15..1106)
                     /locus_tag="VN968_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HXL31180.1"
                     /translation="MKSTDFIVARNDLQQCKFIETSLPDADALPDEALLIKIDRFAFT
                     ANNITYAVLGDQLKYWQLFPAPENFGNIPVWGFGEVIASRHPGIAIGERLFGYFPMAT
                     HLVMEAADVSKRGLRDAAAHRQGVAPVYNAYARVGGDPAFAGRQGDYQALLRPLFMLS
                     FLVDDFLSENEFYGARSVMLSSASSKTAFGLAHLLHTQHRDIRVIGLTSAANFDFVRS
                     LGCYDDVVTYDRVTSLPPDSPVAFVDMAGNSALRANLHQHFGDEMKYSGRIGLTHRAS
                     TPDEAELPGARPSWFFAPDQIRKRAREWGPGGVDTRFGKAWSGFAPKLDRWITVTEGR
                     GEAAVRQVYLDTLNARVPPDQGHILSLGT"
     gene            complement(1103..1849)
                     /locus_tag="VN968_18095"
     CDS             complement(1103..1849)
                     /locus_tag="VN968_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HXL31181.1"
                     /translation="MPLHLNSQADLEQAIHALVKQDPRLKPIFEVAGMPALRRREPGF
                     AGLAAIVSGQQLSTASAAAIWARVSAAFDPFDHETLRRARADRLGRLGLSAAKIKTLK
                     GVARELATGRLNLDVLANEDADAAHNTLTALHGIGPWTADVYLLFCLGHGDAWPAGDL
                     AVQEAVKIGLGLKTRPTVKEMAPLAEPWRPLRGAAAHLWWTYYRVLKKREGVIGGPQK
                     ANAVLNKIPVAKRSSAQAKIAAAKLPGSNK"
     gene            2018..2890
                     /gene="gluQRS"
                     /locus_tag="VN968_18100"
     CDS             2018..2890
                     /gene="gluQRS"
                     /locus_tag="VN968_18100"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HXL31182.1"
                     /translation="MPPPTFRFAPSPNGYLHLGHALSALLNFDSARRTGGRFLLRIED
                     IDVTRCRPEFETAIYEDLAWLGIAWETPVRRQSEHLVEYRDAVEKLAAEGLVYPSFES
                     RGEIARLVAQREVEAPWPRDPDGTPLYPGTAKSLLADQRARLVKSGALYALRLDMAAA
                     RARAGDLAWLEHGKGPDGETGRVAARPQAWGDVILARKEAPTSYHLSVVIDDARQGVT
                     EVVRGEDLFWSTSVHRLLQQLLGLPQPVYRHHRLVRDGEGRKLSKSTMATGLRELRAA
                     GATPADIRRGAGLP"
     gene            3139..4284
                     /locus_tag="VN968_18105"
     CDS             3139..4284
                     /locus_tag="VN968_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL31183.1"
                     /translation="MIPVPSMVEAALAAFAHEVRTPLTGILAISNLLATSDLDERERR
                     WVDTIKAGAEHLASLATLFVDAARSGNAAAAVRQDFFDLRALARSIGDSLAGRAAAKG
                     LQCTIDISEKLPAPVVGDAVRLRAALENLIDNAVKFTETGGVALAVKPVRGPRGKVGV
                     SFAISDSGIGLTLNEIKRLFRPFSQANVSIASRFGGAGLGLSSVKQLARVMGGDIVVS
                     QRRNGGATFILTVTLNRASVSKVAGVDSENDALSDPERPLRVLSVEDNPFGRVVLNAV
                     LTELGHQAEFIGRGEAAAERIAQGAFDAVLMDMVLPGINGIEAIKQIRELEAPLGGIA
                     IIGVSGRGDDEAASRAAGADAFLVKPVSPRALATALLEATRRAATAT"
     gene            complement(4211..5071)
                     /locus_tag="VN968_18110"
     CDS             complement(4211..5071)
                     /locus_tag="VN968_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXL31184.1"
                     /translation="MDAFYTFLADDGWAIASHIALSTLMALFPFLIVLTSLAGFFGSQ
                     ELADQAAELMLETWPKQVADSLSGQIHDVLTTTRGDALTIGVVLAVYFASNGVESLRV
                     ALNRAYSVIEPRRWYWLRLESIGYTLIAAFTALAMAFLIVLGPLMLEAARRYIPLIVE
                     TNENFLNLSRYGIAIAALVVALFVLHAWLPAGRRGFLQILPGIIFTLVASLLSGIVFG
                     QYLARFANNYVTMYAGLASVIIALVFLYFIAAIFVYGGELNAAIIKSRLPHGVSLQAA
                     QSLKPLDSQA"
     gene            5252..5446
                     /locus_tag="VN968_18115"
     CDS             5252..5446
                     /locus_tag="VN968_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HXL31185.1"
                     /translation="MASFLSSILLPVAVGAVALVLLLGLINMMRGGSPNRSQKLMQLR
                     VLLQFVAIVITMLAVWAMGR"
     gene            5460..6032
                     /locus_tag="VN968_18120"
     CDS             5460..6032
                     /locus_tag="VN968_18120"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXL31186.1"
                     /translation="MVVLNRIYTRTGDDGTTALGSGERRPKYDLRVAAYGTVDETNAA
                     IGIVRLHLADARDLDAMLGLIQNDLFDLGADLAVPQREGKAERLRMLSTQVERLEHDI
                     DSLNTRLAPLTSFVLPGGTPAAAHLHLARTICRRAERMMVELAAKPNEPVNDAAIQYM
                     NRLSDFLFVASRSVNDNGAGDVLWVPGQNR"
     gene            6150..6899
                     /locus_tag="VN968_18125"
     CDS             6150..6899
                     /locus_tag="VN968_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HXL31187.1"
                     /translation="MKVLVPVKRVVDYNVKVRVKSDGSGVELSNVKMSMNPFDEIAVE
                     EALRLREAGKATEVVVVSIGPAQAAETIRTGLAMGADRGILVKAEGNVEPLAVAKILK
                     AIADEEKPGLIILGKQAIDDDSNQTGQMLAALLGWSQATFASKLEVDGTDFKVTREVD
                     GGLQTLKLKGPAIVTTDLRLNEPRYASLPNIMKAKKKPIADKSASDYGVDLTVHLEIL
                     KTSEPPGRKGGVKVKDVTELVSKLKNEAGVL"
     gene            6900..>7756
                     /locus_tag="VN968_18130"
     CDS             6900..>7756
                     /locus_tag="VN968_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HXL31188.1"
                     /translation="MTTLLIAEHEHSSLKDSTNKALTAASQLGAEVHVLVAGGGEGTK
                     AAAEAAAKLAGVAKVLLAEGDAYAHDLAEPLAALIVALAPSYDAFVAPATSRFKNVMP
                     RVAALLDVMQVSEITKVVSPDTFERPIYAGNAIQTVKSKDAKKVITVRTSTFAAAGDG
                     GSATVENAAPAADPGLSSFVGEEVAKSDRPELTSAKIIVSGGRAMQSRENFAKYIEPL
                     ADKLGAGVGASRAAVDAGYAPNDWQVGQTGKVVAPELYIAIGISGAIQHLAGMKDSKV
                     IVAINKDEDA"
BASE COUNT         1476 a         2405 c         2527 g         1348 t
ORIGIN      
        1 gacgagacgc cctgctatgt ccccagcgac aggatgtggc cctgatccgg cggtacgcgg
       61 gcattcaagg tatcgagata gacctgcctc accgccgcct cgccgcgacc ttcggtgaca
      121 gtaatccacc gatcgagctt cggcgcgaag cctgaccagg ctttgccgaa ccgcgtgtcg
      181 acgccgccgg gtccccactc cctggcgcgt ttgcggatct ggtcgggcgc gaagaaccat
      241 gacggcctgg cgccgggcaa ttccgcttcg tcaggcgtgg aggcgcggtg ggtgagcccg
      301 atgcggcctg agtatttcat ctcgtcgccg aaatgctggt gcaggttcgc gcgcaacgcg
      361 ctgttacccg ccatgtcgac gaacgcgacg ggtgaatccg gcggcagcga ggtcacgcgg
      421 tcataggtca ccacgtcgtc gtagcagccc agcgacctga caaaatcgaa attggccgcc
      481 gaggtgagcc cgatcaccct gatatccctg tgctgcgtat gcaacagatg cgcgaggccg
      541 aaggcggtct tgctggaggc gctggaaagc atcacgctgc gcgcgccgta aaattcgttc
      601 tcgctcaaga aatcgtcgac caggaacgac agcatgaaca gcggccgcag cagcgcctga
      661 taatcgccct gccgtccggc aaaggccgga tcgccgccga cccgcgcata ggcattgtag
      721 accggcgcca ccccttgccg atgcgcggcg gcgtcgcgca ggccgcgctt gctgacgtcg
      781 gcggcctcca tcaccagatg cgtggccatc gggaaataac cgaacaggcg ttcgccgatt
      841 gcgatgcccg gatggcgcga ggcgatcacc tcgccaaatc cccataccgg aatgttgccg
      901 aagttttccg gcgccggaaa cagctgccag tatttcagct ggtcgcccag caccgcgtat
      961 gtgatgttgt tggcggtgaa ggcgaagcga tcgatcttga tcagcagcgc ttcgtcgggc
     1021 agggcgtcgg cgtctggcag cgatgtctcg atgaatttgc attgctgcag atcgttgcgt
     1081 gcaacgatga agtcggtgga tttcatttgt tcgatcccgg cagttttgct gccgcgatct
     1141 ttgcttgtgc tgatgaccgt tttgcaacag gtatcttgtt taaaacggcg ttcgcttttt
     1201 gcggtccgcc gatcacgcct tcgcgctttt tcagcactcg gtagtaggtc caccacagat
     1261 gcgccgcggc gccgcgcaac gggcgccacg gctccgccag cggcgccatc tctttcacgg
     1321 ttggccgcgt cttgagaccc aatccgatct tcacggcttc ctgcacggcg agatcgccgg
     1381 cgggccacgc gtcgccatgg ccgaggcaaa acaacagata gacgtcggcg gtccacgggc
     1441 cgatgccatg cagcgctgtc aacgtgttgt gcgcggcgtc ggcgtcctca ttcgccagca
     1501 cgtcgagatt cagtctcccg gtggccagtt cgcgcgcgac acccttcaag gtcttgatct
     1561 tcgccgccga cagtccgagc ctgcccagac ggtccgcccg ggcccgacgc agggtctcgt
     1621 gatcgaacgg atcgaatgcg gcgctgacgc gggcccagat cgcggcggca ctggcggtgg
     1681 agagctgctg gccgctgacg atcgccgcaa ggccggcaaa accgggttcg cgccgccgca
     1741 gcgccggcat gcctgcgacc tcaaagatgg gcttgagccg cgggtcctgt tttacaagcg
     1801 cgtggatggc ctgttcgaga tcggcctggc tgttgaggtg gaggggcatg tggcggagat
     1861 caatcaactt ggtggggacg tcatgcgcgg gcttgacccc ggcatccatc tcatatcgta
     1921 acagactttg aacgatagat tgccggtgcg cagacaaatt tacgcagtct gcgcaaggca
     1981 gactcctata tccggcaatg acaggtgtga aaattcaatg ccgccgccca ccttccgctt
     2041 tgcgccgagc ccgaacggct atctgcatct tggccacgcg ctttcggccc tgctgaattt
     2101 cgattccgcg cggcgcaccg gcggccggtt tttgctgcgc atcgaggata tcgacgtaac
     2161 ccgctgcagg ccggaattcg agaccgcgat ctatgaagac cttgcctggc tcggcatcgc
     2221 ctgggaaacg ccggtgcggc ggcagtcgga acatcttgtt gaataccgcg acgcggtcga
     2281 aaaactcgct gccgagggtc tggtctatcc gagtttcgaa agccgcggcg agattgcaag
     2341 actggtggcg cagcgggagg tcgaggcgcc ctggccgcgc gatccggacg gcacgccgct
     2401 ctatcccggt actgcgaaat cgctcctggc cgatcagcgc gcgcgattgg ttaagtcggg
     2461 cgcgctttat gcgctgcggc tggacatggc ggcagcgcgc gcgcgtgccg gcgatcttgc
     2521 ctggctggag cacggcaagg ggcccgatgg cgagaccggc agggtcgccg cccggccgca
     2581 ggcctggggc gacgtcatcc tggcgcgaaa ggaggcgccg accagctatc atctctcggt
     2641 ggtgatcgac gacgcccggc agggcgttac ggaggtggtg cgcggagagg atctgttctg
     2701 gtcgaccagc gtccaccggc tgctgcagca attgctcggt ctgccgcagc cggtctaccg
     2761 gcaccaccgt ctcgtccgcg acggcgaggg gcgaaaactc tcgaagtcga ccatggccac
     2821 gggcctgcgt gaactgcgcg ccgcgggagc gacgccggcc gacatccgcc gcggcgcggg
     2881 tctgccttaa ttgcggcata actatcccaa gaactatccc aagaactatc caaagaaacc
     2941 ataccaagaa ctatcccaag ttgaaaattc ctcgcgcgtg actcccgcgg cccgaccgtg
     3001 gcatgctggc agcggccacg gtggggattc atggcggcaa aaccgcgctt aaagcgccca
     3061 atgcggccgc ccaaagggcg gctgaaaaaa cgcgttccag ccgttgccgc ggctaacgcg
     3121 caaggcaagc gcgccgccat gattcctgtc ccaagtatgg tcgaagcggc gctcgcggcg
     3181 ttcgcgcacg aggtccgcac gccgctgacc ggaattctgg ccatcagcaa tctgctcgcg
     3241 acctcggacc tcgacgagcg cgagcggcgc tgggtcgata ccatcaaggc gggggccgaa
     3301 caccttgcca gcctcgcgac cttgttcgtc gatgccgcgc gaagcggcaa cgccgcggcc
     3361 gcggtgcggc aggacttctt cgatctgcgg gcgctggcgc gcagcatcgg cgattcgctg
     3421 gccggccggg cggcagcaaa gggccttcaa tgcacgatcg atatttcgga gaagcttccg
     3481 gcgcccgtgg tcggcgatgc ggtccggctt cgcgccgcgc tggaaaacct gatcgacaat
     3541 gcggtgaagt tcacggaaac cggcggtgtc gcgctggcgg tcaagccggt gcgcggcccc
     3601 agaggcaaag tcggcgtcag ctttgcgatt tccgacagcg gcatcgggct gacgctcaat
     3661 gagatcaaac gcctgttccg gccgttctcg caggccaatg tcagcatcgc gtcccgcttc
     3721 ggtggcgcgg ggctcggatt atcgtcggtc aagcagctcg cgcgcgtcat gggcggcgac
     3781 atcgtcgttt cgcaacggcg caacggaggc gccacattca tcctgaccgt gaccttgaac
     3841 cgcgcaagcg tatcgaaggt ggccggcgtg gacagcgaaa atgatgcatt gtcggatccg
     3901 gagcggccgc tgcgcgttct cagcgtcgag gacaatccgt tcggccgcgt cgtgctcaat
     3961 gcggtcctca ccgaactcgg tcatcaggct gaattcatcg ggcgaggcga ggccgcggcc
     4021 gagcgtatcg cgcaaggcgc gttcgacgcg gtgctgatgg acatggtgtt gccgggcatc
     4081 aacggcatcg aggccatcaa gcaaattcgc gagctggagg cgccgctcgg cggcattgcc
     4141 atcatcgggg tgtccggccg cggcgacgat gaagccgcat cgcgcgccgc cggcgccgat
     4201 gcctttctgg tcaagcctgt gagtccaagg gctttagcga ctgcgctgct tgaagcgaca
     4261 cgccgtgcgg caaccgcgac ttgatgatcg ccgcgttcag ctcgccgcca tacacgaaga
     4321 tcgcggcgat gaaatacaga aacaccagcg cgatgatgac cgacgcaagg ccggcataca
     4381 tggtgacgta gttgttggcg aagcgcgcga gatattgtcc gaacacgatg cccgacagca
     4441 gcgacgccac cagggtgaag atgatgccgg gcaggatctg cagaaacccg cggcggcccg
     4501 ccggaagcca ggcgtggaga acaaacagcg cgacgaccag cgccgcgatg gcgatgccgt
     4561 agcgggagag gttgaggaaa ttctcgttgg tttcgacgat cagcggaatg tagcggcggg
     4621 ccgcctccag catcaggggc ccaagcacga tcaggaacgc catcgccagg gcggtgaagg
     4681 cggcgatcaa ggtgtagccg atcgattcca gccgcagcca gtaccaacgc cgtggctcga
     4741 tcaccgaata agcgcggttg agcgcgacgc gcaggctttc gacgccattc gaggcgaagt
     4801 acaccgcgag cacgacgccg atggtcagcg cgtcgccgcg ggtcgtcgtc agcacatcgt
     4861 gaatctggcc cgacagcgag tcggcgacct gcttcggcca ggtctccagc atcagttccg
     4921 cggcctgatc ggcgagttcc tgcgatccga aaaagccggc cagcgacgtc agtacgatca
     4981 gaaacggaaa cagcgccatc agcgtcgaca gcgcgatatg gcttgcgatc gcccagccgt
     5041 cgtcggcgag aaacgtgtag aacgcatcca tgccgacatg aaagatgtag cgaagctgcc
     5101 tcacatttca ccttgaccaa tggaacatgc gctcgatccc gcgaaccgcg ctaccttctg
     5161 atattattga gctaaaagcc agatcgttgc atcggagctg cgccccgatg cgggcgatgg
     5221 cggaccgccc tgcgcggtgt tatgtaccgt catggcatca tttctgagtt cgatcctcct
     5281 tccagttgcg gtcggcgccg ttgcgctggt gctgctgctg ggtcttatca acatgatgcg
     5341 gggcgggtcg ccgaaccggt cgcagaaact gatgcagttg cgggtattgc tgcagttcgt
     5401 tgcgattgtc atcaccatgc tcgcggtctg ggcgatgggc cgctgaagga gacaaaagaa
     5461 tggtcgtcct caatcgaatc tacacccgga ccggtgacga cggcacgaca gcgctcggca
     5521 gcggcgaacg ccgcccgaaa tacgacctgc gcgtcgccgc ctatggaacc gtcgacgaga
     5581 ccaacgcggc catcggcatc gtgcgcctgc atctcgccga cgcgcgcgat ctcgatgcga
     5641 tgctgggcct gatccagaac gatctgttcg acctcggcgc cgatctcgcg gtaccccagc
     5701 gcgagggaaa ggcggaacgc ttgcggatgc tgtcgaccca ggtcgaacgc ctcgagcacg
     5761 acatcgacag cttgaacacg cggctggcgc cgttgacctc ctttgtgctg cccggtggca
     5821 cgccggcggc ggcgcatctg catctggccc gcacgatatg ccgccgggcg gaacgaatga
     5881 tggtggaact cgcggccaag ccaaatgaac cggtcaacga cgccgcgatc cagtatatga
     5941 acaggctgtc ggacttccta ttcgtcgcca gccgatccgt gaacgataac ggcgccgggg
     6001 acgtgctgtg ggtgcccggc cagaatcgtt agcgccttca agtgaggcag tcgggggtga
     6061 tttttgggct tcgcgcgttg accggggttg gcgggacctt taggttccgc gcccatgata
     6121 aaacctgaaa ttcaagcgaa agagggtcga tgaaggtcct ggtaccggta aagcgggtgg
     6181 tcgattacaa cgtcaaggtc cgcgtcaaga gcgacggatc gggcgttgag ctgtccaacg
     6241 tcaagatgtc gatgaatccg ttcgatgaaa tcgccgtcga ggaagccttg cgcctgcgag
     6301 aggccggcaa ggcgaccgag gtggtggtgg tttcgatcgg cccggcgcag gcggcggaaa
     6361 ccatccggac cggtctcgcg atgggcgccg accgcggtat tctggtcaag gcggagggca
     6421 acgtcgaacc tttggcggtg gcgaaaatcc tcaaagccat cgccgacgaa gagaagcccg
     6481 gcctgattat tctcggcaag caggcgatcg acgatgatag caaccagacc ggccagatgc
     6541 tggccgccct gctcgggtgg tcgcaggcga cctttgcctc caagctcgaa gtcgatggaa
     6601 ccgatttcaa ggtgacgcgc gaggtcgacg gcggcttgca gacgttgaaa cttaaaggcc
     6661 cggcgatcgt aactaccgat ctgcggctga acgagccgcg ctacgccagt ctgcccaata
     6721 tcatgaaggc aaagaagaag ccgatcgccg acaagagtgc atccgattac ggtgtcgatc
     6781 tcaccgtcca tctggagatc ctcaagacgt ccgaaccgcc gggccgcaag ggaggcgtca
     6841 aggtcaagga tgtcacggag ttggtgtcga agctcaagaa cgaagccggg gttctctaga
     6901 tgaccacgct gttgatcgcc gaacacgaac actcaagctt gaaggactcc accaacaagg
     6961 cgctgaccgc ggccagccaa ttgggcgccg aggtccacgt gctggtcgcc ggcggcggcg
     7021 agggcaccaa ggcggccgcc gaggccgcgg ccaagcttgc tggcgtcgcg aaggttttgc
     7081 tcgccgaagg cgatgcctat gcgcatgacc tcgccgagcc gctggccgcg ctgatcgtgg
     7141 cgctggcccc ttcctatgac gctttcgtcg cgcccgcgac gtcgcgcttc aagaatgtca
     7201 tgccgcgggt cgcagcgctg ctcgatgtga tgcaggtatc ggaaatcacc aaggtggttt
     7261 cgcctgatac cttcgagcgg ccgatctatg ccggcaacgc catccagacg gtgaaatcga
     7321 aggacgccaa gaaggtcatc accgtccgca cctcgacctt cgccgctgcc ggagacggcg
     7381 gcagcgcaac ggtggaaaac gccgcgccgg cggccgaccc cggtctttcc agttttgtcg
     7441 gcgaggaagt tgccaagagc gatcgccccg aactgacgtc ggccaagatc atcgtctccg
     7501 gcggccgcgc catgcagagc cgcgagaatt ttgccaaata tatcgagccg ctcgccgaca
     7561 agctcggcgc cggcgtcggc gcctcgcgcg ccgcggtcga tgccggatat gcgccgaacg
     7621 actggcaggt cggccagacc ggcaaggtgg tggcgccgga actttatatc gccatcggca
     7681 tttcaggcgc cattcagcat cttgccggta tgaaggattc caaggtgatc gtcgcgatca
     7741 acaaggacga ggatgc
//
LOCUS       DATNOI010000453        11472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_20815,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000453 DATNOI010000000
VERSION     DATNOI010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11472
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20815"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1955)
                     /gene="atzF"
                     /locus_tag="VN968_18135"
     CDS             complement(159..1955)
                     /gene="atzF"
                     /locus_tag="VN968_18135"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HXL31189.1"
                     /translation="MTETVAAIVAAHRAGILSPAQTVARSFARIRDHNDPAIFISLRD
                     EKEALAEAEGLTAKAGAQLPLYGVPVAVKDNIDVAGLATTAACPAFAHSPSHDATSVA
                     RLRAAGAIIIGKTNLDQFATGLVGVRSPYGIPTNPMRGDLIPGGSSSGSAVAVSAGLV
                     PLALGTDTAGSGRVPALLNNVVGLKPSLGLVSTAGLVPACRTLDCVSVFSLTVDDAMT
                     ALVAMAGPDRADPFSRNRALAAMAAFPKLLRLGVPRNGQLIFFGDKASEKAYGDALKR
                     WTALGAELVEFDLEPLYETARLLYEGPWVAERYLVIRNLLASSPDSIHPVTREITIAG
                     ARLSAADTFSALYRLQALRRIAERAFAHIDALVLPTAPTVYSTAQVLANPIELNSRLG
                     TYTNFVNLLDLCGLALPAAIRPDDIPFGITLLAPAGHDAQLASIGRVFHADTKLTIGS
                     KKLPQPPLATVPAGLSGDEIAIAVVGAHLSGMALNNELKALGGRLLEAASTAPDYRLY
                     ALDTSPPKPGMLRVAAGAGSSIELELWALSAASFGKFVAAIPPPLSIGTLRLADGRGV
                     KGFIVEAAEINGARDISAFGGWRAFVTEAAAV"
     gene            2361..4229
                     /locus_tag="VN968_18140"
     CDS             2361..4229
                     /locus_tag="VN968_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HXL31190.1"
                     /translation="MSTAAASHRENSETLFATPEIVADRRADGTIWLKSTMPLRQDAR
                     CVGDWLEQWEQKSPERIFLAERSDAGAPWTTVTYRDALRQVRSVAAWILAQGLSAERP
                     LVILSDNSIEHALFALAAMHVGVPAAAISPAYSLVSKDFDKLKSMIGLLDPGAIHVSN
                     MKPFAAALAAIKPLHRAVIVGGDANGGDAISFQSVVSTAETEAVAKAFAAVTPDSIAK
                     FLFTSGSTGTPKAVINTQHMLTSSQQARAQTWPFLEGARDDLVILDWLPWSHTFGANH
                     NFNLVLRNGGSLYIDGGKPAPGLFATSLANLRSVMPTVYFNVPRGFDMLIAALRGDDE
                     LRRRFFGEVKFAFYAGAALPQNLWDAMEELASGTVGRALPMVSAWGSTETSPLATDCH
                     FQAQRSGNIGVPIPGTELKLVRSGDKLEVRVRGPNVTPGYWKAPELTAQAFDADGFYR
                     IGDAVTFADPDCPELGLFFDGRVAEDFKLNSGTWVSVGTLRVAGITALAPLAQDIVVA
                     GHGGDHVCFLVFPNVAACRAQAGSPEGADVREVIGHQKVREAIVSGLARLRAQSGGGS
                     SGHATRALLLAEPASVDGGEITDKGYINQRAVLTRRAAAVAVLEDDASTEWIGCAG"
     gene            4335..4769
                     /locus_tag="VN968_18145"
     CDS             4335..4769
                     /locus_tag="VN968_18145"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HXL31191.1"
                     /translation="MTPYTFCANLIIDWLLTDIKAGRIANTEGTAFILERGHENNAEA
                     ELNFYNVRKLHKLESILQSISFIPKEECRAIQMADLFAFYSRRHGVEMEAAPPDQKQR
                     IQRTPGTMLNIITETVRHRAFVATDFGPSAAGSRFFGGDGDS"
     gene            complement(4845..6128)
                     /locus_tag="VN968_18150"
     CDS             complement(4845..6128)
                     /locus_tag="VN968_18150"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL31192.1"
                     /translation="MTEAQARSIKPHRVVIVGAGFGGLEAALRLAGAPVSITLIDRRN
                     HHLFQPLLYQVATASLATSEIAWPIRYLLRNRPDVTTLYATVTGVDAQAKRVLLDDGD
                     GLPYDTLIVATGARHAYFGHDEWEPFAPGLKTLEDATTLRRRILVAFERAERETDPQR
                     RAALLTFVIIGAGPTGVELAGTIAELAQDTLPPDFRNIDTHRTRVVLVEAGPRVLAGF
                     PDDLSAYAQRSLKSIGVEVMLGQAVTECNADGVVYGGNRLEAKTIIWAAGVRASPAAE
                     WLGAPADRAGRLQVLPDLTVPGHPDIFAIGDTVTIDGPDGKPVPGIAPAAKQQGRYAA
                     DTIKARLSGGTPTPFRYQHAGSLAQIGKRKAVIDFGRIKLRGTVAWWIWGIAHIYFLI
                     GLRNRLSVAISWLWIHARDQRAARLITQGSSKVVG"
     gene            complement(6312..7718)
                     /locus_tag="VN968_18155"
     CDS             complement(6312..7718)
                     /locus_tag="VN968_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HXL31193.1"
                     /translation="MQEANARGEKNWVLGVTALASFMVALDTQVLTAALATLRADFGA
                     PMEALQWTVNAFNLSFAVLLLTGAALGDRFGRRRLFAAGIGLFMASSVACALSTSILA
                     LIAARAAQGTGAALVMPLGMALLSTAFPREERARALGIFSGVVGIAVLAGPAVGGAIT
                     EGLGWQWIFWINLPIGLIAVVMVITRLRESFGPAAALDIPGLALVAASAFAVVWGLLR
                     GNAAGWNSAEVLTALVVGLLLAAAFVAWEWRASAPMVPMRLFRSRAFSSGIVAGFLLY
                     GALYGVLFLLPQFMQTALGYGPFGVGLRLLPWTATLFMTAPIAGNVVNRLGERPLVAI
                     GLLMQATGLGWIAMIITPGVAYATMIAPLVVAGVGVSMAMPAVQNAVLGSVNVVEMGK
                     ASGVFNMGRFLGGMFGIALLVATFSANGTTDSPANFSAGFAAAMTVAAVLSLLGALAG
                     MWLPARDRVAAAQVPRNA"
     gene            7895..8200
                     /locus_tag="VN968_18160"
     CDS             7895..8200
                     /locus_tag="VN968_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31194.1"
                     /translation="MKRTLIRYKTNPESADKNAELIRGVFEQLKADGPEGVRYLSLRL
                     EDGTFVHFVETESDSANFLPSLAAFKAFQSGIRERCIEPPQAGSVTIVGNYRMLREP"
     gene            8229..9119
                     /locus_tag="VN968_18165"
     CDS             8229..9119
                     /locus_tag="VN968_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXL31195.1"
                     /translation="MKRVPDAPAAAGGFDGLLARMRPKLHRYCARMVGSVIDGEDVLQ
                     DALIKAVESHATAGAIGNPEGWLFRIAHNTALDFLRRRNRQQALRSAEEVDMIADPTD
                     AVLSRQIASASLRTFMRLPVAQRASVILMDVLGCSLTEICEVMDFSLPAVKAALHRGR
                     TQLRELADEPDDRPPPTLSDADRKRLGVYVAHFNARDFDAIRAMIADDVRLDLVNKTR
                     MNGKIEVSKYFGNYSKVRDWHLVPGLVEGHPAILVFDPSEPGSAPKYFMLLQWSADKV
                     ATIRDFRHAAYVIDGAEYLI"
     gene            9145..9873
                     /locus_tag="VN968_18170"
     CDS             9145..9873
                     /locus_tag="VN968_18170"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HXL31196.1"
                     /translation="MATNEIPHPPIVSHDQWLAERKKLLAHEKELTRQHDRVNAERRR
                     LPMVRIEKDYVFDGANGKQRLKDLFEDRRQLVIYHFMFDPSWENGCPGCTGYANALGD
                     LSMLRKCDTTFAMVSRAPLAKLEAYKAKKGWSVPWYSSFGSDFNYDFHVTNDEKVAPV
                     EYNYRNKAELEANKVPNAISGEEHGLSVFFRIGDDVFHTYSAYARGTEGRTGSRALLD
                     MTPYGRQQDFEDSPPGWPQRPTYG"
     gene            10201..11373
                     /locus_tag="VN968_18175"
     CDS             10201..11373
                     /locus_tag="VN968_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31197.1"
                     /translation="MRTAFWLAGATVLALANPALAADSIKIGFVSTFSGPTAVIGNDM
                     RNSFELALDHMGRKMGGLPVEVIYEDDQQKPDVGKQKTEKLVQSDKVDFIAGYIWSNV
                     LLASLKTAVDSQTFLISANAGPSQLAGELCSPYVFSTSWQNDQTPQAMGLYMNQKGVK
                     SVFLIGPNYAAGKDMLAGVKSTFKGEVKGEEYTVWPSQLDFSAELSKARASGAESIFV
                     FYPGAAGVQFLNQYAQAGIKAQIPLYTAFTIDELSLPLQKDNALGVPGAQEWVNDLPN
                     DENKRFVADYRKKYPNLRPTFYGAQSYDAAQLINSAVVAVKGDTSKKDAMKAEMEKAN
                     FKSVRGPFKYGKNHIPIQSFYLQDVVKDADGQLSLKTIATIVENSQDRFADKCPMK"
BASE COUNT         2250 a         3599 c         3647 g         1976 t
ORIGIN      
        1 gcctgaacgc cacattccga actcgatcgc gaccatgcgc gtgcggttga ttaacgcgct
       61 catcagaacc ctgccgcgat gtccctgttg cggtaccaac cccgcaacat acagacgacg
      121 gattatgacg cagtaagatt attcagctct acgtcctttc aaaccgccgc cgcttccgtc
      181 acaaacgcgc gccagccgcc gaacgccgag atatcgcgtg cgccattgat ctcggcggct
      241 tcgacaataa aacctttcac gccgcggcca tcagccagac gcaacgtgcc gatcgacagc
      301 ggcggcggga tcgcggcgac gaactttccg aacgaagccg ccgacaatgc ccatagctcc
      361 agctcgatcg acgatccggc tcccgcagcc acccgcagca tgcccggctt cggcggcgag
      421 gtgtcgagcg cgtagagcct gtagtccggc gcggtgctgg cggcctcgag cagacgtccg
      481 ccgagcgcct tcaattcatt gttgagcgcc atgcccgaga gatgcgcgcc gaccaccgcg
      541 atggcgattt catctccgct caggcccgcc ggcaccgtgg cgaggggagg ctgcggcagc
      601 ttcttgctgc cgatggtcag tttggtgtcg gcatggaaga cgcggccgat gctcgccagt
      661 tgcgcatcgt gtcccgccgg cgcgagcagc gtgatgccga acgggatatc gtcggggcgt
      721 atcgccgccg gcagggccag accgcagagg tcgagcaaat tgacaaaatt ggtgtaggtt
      781 ccgagcctgg agttgagttc gatcggattg gccagcacct gggcggtaga atatacggtc
      841 ggcgccgtcg gcagcaccag ggcgtcgata tgggcaaagg cgcgctcggc gatgcggcgc
      901 agcgcctgca gccggtacag cgcgctaaat gtatcggcgg cgctcagccg tgcaccggcg
      961 atcgtgatct cgcgcgtcac cggatggatc gaatccggcg atgacgccag caaattgcgg
     1021 atcaccagat agcgctcggc gacccacggt ccttcataga gcagccgcgc ggtctcatag
     1081 agcggctcga gatcgaactc gaccagttcg gcgccgagcg cggtccagcg cttcagcgcg
     1141 tcgccgtagg ccttttccga agccttgtcg ccgaagaaga tcaactggcc gttgcgcggc
     1201 acaccaagcc tgagcagctt cggaaacgcg gccatcgccg ccagcgcccg gttgcgcgaa
     1261 aacggatcgg cgcggtcggg cccggccatc gccaccaacg ccgtcatcgc gtcatcgacg
     1321 gtcagcgaga ataccgacac gcaatccagc gtccggcatg ccggcaccag acccgcggtg
     1381 gaaaccagcc cgaggctcgg cttcaggccg acgacgttgt tgagcagcgc aggcacacgg
     1441 ccggagccgg ccgtgtcggt gccgagcgcc agcggcacca gcccggccga caccgctacc
     1501 gccgatcccg aactcgatcc gcccggaatg aggtcgccgc gcatcggatt ggtgggaatg
     1561 ccatagggcg agcgcacgcc gacgaggccg gtggcgaact gatcgaggtt ggtcttgccg
     1621 atgatgatgg cgccggccgc gcgcagccgc gccaccgagg tcgcatcatg cgacggcgaa
     1681 tgggcgaagg ccgggcaggc ggcggtggtg gctaagccag cgacatcgat attgtccttg
     1741 acggcaaccg gaacgccgta cagcggaagc tgcgcgcccg ctttggcggt caggccttcc
     1801 gcctccgcca gcgcttcctt ttcgtcgcgc aggctgatga agatcgcggg atcgttatga
     1861 tcgcgaatgc gggcaaagct gcgcgcgacg gtttgcgcag gcgagaggat tccggcgcga
     1921 tgcgcggcga caatcgcagc gacggtttca gtcatgcgcc accctgatcg ggagagaact
     1981 tattcggcag gcgcggcaag ttgtatctcc ggatgagcaa cagaaagggg cgccaaggtt
     2041 cgtcaaggtt cgccacggct gcgttccagg agacgggaat accaccggat cgaagcaagc
     2101 ggtgtgccat tgtgtacacc gtcggggaac gattggtata cgaaattgct gtcgtgttat
     2161 cagatgcttg agcgaatttc acggcgacgc tgggagtggg cggcccggaa agcccgaatt
     2221 gaacttgccc attttattgg cagagttatt ccttgcaacc cttgcccggc cccggtgcgc
     2281 gatgacgcat tgccggggcg cgcggttctc gcgtagagca ggcgccagcc cctggccaaa
     2341 gagggcatca gaggaaaccg atgagcactg ctgccgccag ccatcgcgag aattccgaaa
     2401 cgctgtttgc cacgcctgag atcgtggcgg atcgccgcgc cgacggcacc atctggctga
     2461 agtcgaccat gccgctgcgc caagacgcac gctgcgtcgg cgactggctg gagcagtggg
     2521 agcaaaaatc ccctgagcgg atttttcttg ccgagcgttc tgacgctggc gcgccgtgga
     2581 cgaccgtcac ctaccgcgat gcgttgcggc aggtgcgctc tgttgccgcc tggatcctgg
     2641 cgcaaggttt gagcgccgaa cgccctttgg tcattctctc cgacaacagc atcgagcatg
     2701 cgctgttcgc gctcgcggcc atgcatgtcg gggtgcccgc ggctgcgatc tcgccggcct
     2761 attcgctggt gtcgaaggat tttgacaagc tcaagagcat gatcggcctg ctcgaccccg
     2821 gcgcgatcca tgtgtcgaac atgaaaccat tcgcagcggc gctggcggcg atcaagccgc
     2881 tgcatcgggc ggtgatcgtc ggcggcgacg cgaacggtgg cgatgcgatc tcttttcagt
     2941 cggtcgtctc aaccgctgag accgaggccg tggccaaagc ctttgcggcc gttacgccgg
     3001 acagcatcgc aaagtttctt ttcacctcgg gctcgaccgg cacgccgaaa gcggtcatca
     3061 acacccagca catgctgacc tcgagccagc aggccagagc gcagacctgg ccgtttctcg
     3121 aaggcgcgcg ggatgatctc gtcattctcg actggctgcc atggagccac acgttcggcg
     3181 ccaatcacaa tttcaatctc gtgctgcgca acggcggctc gctctatatc gacggcggca
     3241 aaccggcgcc gggcctgttc gcgacatcgc tggccaatct gcgcagcgtg atgccgacgg
     3301 tctacttcaa cgtcccgcgc ggcttcgaca tgctgatcgc ggcgctgcgc ggcgacgacg
     3361 aattgcgccg ccgcttcttt ggcgaagtga aattcgcgtt ctatgcaggg gcggcactgc
     3421 cgcagaacct ctgggacgca atggaggaac tggcgagcgg gaccgtcggc cgcgcgctgc
     3481 cgatggtctc ggcctggggc tccaccgaga cctcgccgct ggcgaccgac tgccatttcc
     3541 aggcacagcg ctccggcaat atcggcgtgc cgatacccgg caccgaattg aagctggtac
     3601 ggtcaggcga caagctcgag gttcgcgtgc gcggacccaa cgtcacgccg ggctactgga
     3661 aggcgccgga gctgaccgcg caggcgttcg atgccgacgg attctatcgg atcggcgatg
     3721 ccgtgacctt cgccgacccg gattgcccgg agctcggact gtttttcgac ggccgcgtcg
     3781 ccgaggattt caagctcaat tccggcacct gggtcagcgt cggcacgctg cgtgtcgccg
     3841 gcatcaccgc actggcgccg ctggcgcagg acatcgtcgt cgccggccat ggcggcgacc
     3901 acgtgtgctt tttggttttt ccgaacgtcg ccgcgtgccg cgcgcaggcc ggctcgcccg
     3961 aaggcgccga tgtgcgtgag gtgatcggcc accaaaaagt gcgcgaggcc atagtgtcgg
     4021 gactggcaag gctcagggcg caaagcggcg gcggctcgtc cggtcacgcg acgcgggcgc
     4081 tgctgctggc ggagcctgct tcggtcgacg gcggcgagat caccgacaag ggctatatca
     4141 accagcgcgc cgtgctgacg cggcgcgcag ccgcggttgc ggtgctggaa gatgatgcgt
     4201 caaccgaatg gatcggatgt gcgggctgag acgataaaac gcaaaaatca aacacaaaac
     4261 gacaagcagc cgtacgcgga taggtgtgac gggttgcaat atgtgctggg gccgaaagtg
     4321 gtcgaaaacg aacggtgacg ccttatacct tttgcgcaaa tctcatcatc gattggctgc
     4381 taaccgacat taaggcgggt cggatcgcca atactgaagg cactgccttt atcttagagc
     4441 gcgggcatga aaacaatgct gaagctgaac ttaactttta taatgttcgg aaactccata
     4501 agttggaaag catacttcag tcgatttcgt tcataccaaa agaggaatgt cgcgccattc
     4561 agatggccga tctgtttgca ttttattcgc gcaggcatgg cgtagagatg gaagccgcac
     4621 ccccggatca aaagcagaga atccagcgta cgcctggaac gatgttgaat ataatcaccg
     4681 aaaccgttcg tcatcgcgca tttgttgcga ctgattttgg cccatctgcc gcgggctcac
     4741 ggtttttcgg gggcgatggg gatagctaac ggtaccgcga cgttctttca aacggtgctg
     4801 ccgatcgcgt gtagccccgg ccgcactggc tgtgttgcga gaagttagcc gaccaccttg
     4861 ctgctgcctt gggtgatcag ccgcgcggcg cgctgatcgc gggcgtggat ccacagccag
     4921 ctgatcgcga cgctgagccg gttgcgcagc ccgatcagga aatagatgtg cgcgatgccc
     4981 catatccacc acgcaacggt gccgcgcagt tttatcctgc cgaaatcgat cacggctttc
     5041 cgcttgccga tctgcgccag gctgccggcg tgttggtaac ggaacggggt aggggtgccg
     5101 ccgctcagcc gcgccttgat ggtatcggcg gcgtagcgtc cctgctgctt cgcggccggc
     5161 gcaatgccgg ggaccggctt gccatccgga ccatcgatcg tgacggtatc gccgatcgca
     5221 aagatatccg ggtggcccgg taccgtaagg tcgggcagga cttgcaggcg gccggcgcgg
     5281 tcggccggcg caccaagcca ttcggcggcg ggcgaggcgc gcacgccggc ggcccagatg
     5341 atggttttgg cttcgagccg gttgccaccg tacacgacgc catcggcgtt gcattcggtg
     5401 acggcctgtc ccagcatcac ttcgacgccg atgcttttca gcgagcgctg cgcataggcg
     5461 gagagatcgt cgggaaatcc cgccagcacg cgcggcccgg cctcgaccag gacaacgcgc
     5521 gtcctgtggg tgtcgatgtt gcggaagtcg ggcggcagcg tgtcctgcgc tagttcggcg
     5581 atggtgccgg cgagttcgac gccggtcgga ccggcgccga taatcacaaa agtcagcagt
     5641 gccgcgcgcc gctgcggatc ggtctcgcgc tcggcacgct cgaaggcgac cagaatgcgc
     5701 cgccgcaacg tggtggcatc ctccagcgtc ttcagccccg gcgcgaacgg ctcccattcg
     5761 tcatggccga aataggcgtg gcgggcgccg gtcgcgacaa tcagggtgtc atagggcagg
     5821 ccgtcgccgt catcgagcag cacgcgcttg gcttgcgcat ccacgccggt cacggtggcg
     5881 tacaaggtcg tgacatcagg gcgattgcgc aacaggtaac ggatcggcca ggcgatctcc
     5941 gacgtcgcca gtgacgcggt tgcgacctga tacaacagcg gctggaacaa atgatggttg
     6001 cgccggtcga tcagcgtgat gctgaccggc gcgccggcaa gccggagcgc cgcttccaac
     6061 ccgccgaagc cggcaccgac gatcaccacg cgatggggtt ttatggatcg cgcctgcgct
     6121 tcggtcatgg gcttgcggcc tttggtttca gatttccccg tgcatggtat gtaagcgttt
     6181 ggacgcctcc gtccaaggcc gttttgccaa gtccgttttg ccaagtccgg tttgccaagt
     6241 ccggtttgct aatcgatcga tagcgaaaac ccatcgatcg accgcaatca gcagcgttag
     6301 tccatttgat gtcaggcatt ccgcggcacc tgcgcagcgg cgacgcgatc acgcgccggc
     6361 agccacatgc ccgccaacgc gcccagcaac gaaagcactg ccgcaaccgt catcgccgcg
     6421 gcgaagccgg cgctgaaatt cgccggcgaa tcggtggtgc cgttggctga aaatgtggcg
     6481 acaagaagcg cgatgccgaa catgccgccg agaaagcgtc ccatgttgaa gacgccggac
     6541 gccttgccca tctcgacaac attgaccgaa cccagcacgg cattctgaac cgccggcatc
     6601 gccatcgaca cgccgacacc ggcaacaacc agcggcgcaa tcatggttgc gtaggcgacg
     6661 ccgggcgtaa tgatcatcgc gatccagccg agcccggttg cctgcatcag cagcccgatc
     6721 gcgaccagcg ggcgttcgcc cagtctgttg acgacattgc ccgcgatcgg cgcggtcatg
     6781 aacaaggtgg cggtccaggg caacagccgc aggccgacgc cgaacggccc gtagccgagc
     6841 gcggtctgca tgaattgcgg cagcagaaac aacacgccgt acaacgctcc gtacaacaga
     6901 aagccggcga cgatgcccga cgagaacgcc cgcgaccgga acagccgcat cgggaccatt
     6961 ggcgcggatg cgcgccattc ccaggcgaca aatgccgccg ccagcagcaa cccgacgacg
     7021 agcgcggtca gcacctcggc gctgttccag ccggcggcgt tgccgcgcag caggccccag
     7081 accaccgcga atgccgaggc cgcgaccagc gcgaggccgg gaatatcgag ggctgccgcc
     7141 ggcccgaaac tctcgcgcag ccgggttatc accatgacga cggcgatcag cccgatcggc
     7201 agattgatcc agaagatcca ctgccacccc aggccttcgg tgatggcgcc gccaacagcg
     7261 ggccccgcca gcacggcaat tccgaccaca ccgctgaaga tgccgagcgc tcgcgcccgc
     7321 tcttccctcg gaaaggctgt gctcagcaac gccatgccga gcggcatcac caatgcggcg
     7381 ccggtgccct gagcggcgcg cgctgcgatc agcgcgagga tgctggtcga cagcgcgcag
     7441 gcgaccgacg aggccatgaa cagtccgatg cccgccgcga acaggcggcg gcgtccgaac
     7501 cggtcgccga gcgcggcgcc ggtcaacagc agcaccgcga agctgagatt gaaggcgttg
     7561 accgtccatt gcagcgcctc catcggggcg ccgaaatcgg cgcggagggt ggcaagcgcg
     7621 gccgtcaaca cctgggtgtc gagcgccacc atgaaggacg ccagcgcggt cacgccaagc
     7681 acccaattct tttcgcctct cgcgttagct tcctgcatcg catctctccg ccttgtttcg
     7741 gccgcctcat gcggcggttc acggacagga cgaatgggaa gaggcggagg attcggccgg
     7801 aaattatttt ttggcgaccg aatcctctgg ttggtgtcga acgtctatgg gccgagacgc
     7861 gatgattgtg catcgcgggc catggagaga aatgatgaaa cggaccttga tcaggtataa
     7921 aaccaacccg gaatcggccg acaagaacgc cgagctgatc cgaggcgtgt ttgagcaatt
     7981 gaaggcggac gggccggagg gcgttcgcta tctttcgctg cggctggagg atggcacctt
     8041 tgttcatttc gtcgagactg aatccgattc cgccaatttc cttccgagcc tggccgcgtt
     8101 caaggcgttc cagagcggga ttcgcgagcg ttgcatcgag ccgccgcaag ccggcagcgt
     8161 gacgatcgtc ggcaattatc ggatgctgcg ggagccctga gcaccgggct cgcatgttcg
     8221 gaggctgaat gaaacgggtc cctgatgcac cggccgccgc cggtgggttt gacggccttc
     8281 tggccaggat gcggccaaaa ctgcaccgct attgcgcgcg catggtcggc tcggtgatcg
     8341 acggcgagga cgtgctgcag gatgcgctga tcaaggcggt ggagtcgcat gccaccgcgg
     8401 gcgcgatcgg caaccccgaa ggctggctgt tccggatcgc gcacaacacc gctctggatt
     8461 tcctgcgccg ccgcaaccgc cagcaagcgc tccgatcggc cgaggaggtg gacatgattg
     8521 cggacccaac cgatgccgtc ctaagccgcc agatcgcaag cgcaagtctt cgtaccttca
     8581 tgcggcttcc ggttgcccag cgcgccagcg tgatcctgat ggacgtgctc ggctgctcat
     8641 taacggaaat atgcgaggtg atggatttca gcctgcccgc ggtgaaagca gcgttgcatc
     8701 gcggacgcac ccagcttcgc gaactggccg acgagccgga cgatcggccg ccgccaacat
     8761 tgtcggacgc cgatcggaag cgtctcggcg tctatgtcgc gcacttcaac gcgcgggact
     8821 ttgacgcgat ccgcgccatg atagccgacg acgtgcgcct cgacctcgtc aacaagacac
     8881 ggatgaacgg caagatcgag gtctcgaaat atttcggcaa ctattcgaag gtgcgcgact
     8941 ggcatctggt gcccgggctg gtggaaggac atcccgccat cctggtgttc gatcccagcg
     9001 aacccggctc agcgccgaaa tatttcatgc tgctgcaatg gtcggccgac aaggtcgcaa
     9061 ccatccgcga tttccgccac gcggcctacg tcatcgacgg cgccgaatat ctgatctgat
     9121 cagcttgcga taaggagaac ggacatggcg acgaacgaaa tcccgcatcc cccgatcgtt
     9181 tcgcacgacc aatggctcgc cgaacggaaa aaacttctcg cgcacgagaa agaactcacc
     9241 cggcaacatg atcgcgtcaa tgccgaacgg cgccgattgc cgatggtcag aatcgaaaaa
     9301 gactatgtat tcgacggagc gaacggcaaa cagcgcctga aggacctgtt cgaggaccgc
     9361 cggcagctcg tgatctatca cttcatgttc gatccctcat gggagaacgg ctgccccggc
     9421 tgcaccggtt atgcgaacgc cctcggcgat ctcagcatgc tgcgcaaatg cgacaccacg
     9481 ttcgcaatgg tatcgcgcgc gccgctggcc aagctcgagg cctacaaggc caaaaagggt
     9541 tggagcgttc cgtggtattc gtcgttcggc agcgacttca attatgactt tcacgtcacc
     9601 aatgacgaga aggtagcgcc ggtcgaatac aactaccgca acaaggccga gctcgaagcc
     9661 aacaaggtcc ccaacgccat atcaggcgag gagcacggcc tcagtgtttt cttccgcatc
     9721 ggcgacgatg tttttcacac ctattcggcc tacgcgcgcg gcaccgaagg tcgcacgggt
     9781 tcccgcgcgt tgctcgacat gacaccttac gggcggcagc aggattttga ggactcgccg
     9841 cccggctggc cgcagaggcc gacttacgga taggtcgagg ccgccatctg gccggcacgg
     9901 gtgccgagtc ggcacgttgc ggccgaccgc gctcgcggac ggcgatcgat cccctccgct
     9961 cccaatcaga actataattc ttgccatccg cggcctatac gaattatggt gcatggtcct
    10021 tgcgggccgg ccgtagcgtt aacggacacc gaaggtcgtg cttgccgcga gcctaggatt
    10081 tcgcgcacaa acgccatcaa caagagtggc tcgaaaacgg gaaaagaggc caacaaggct
    10141 ctccggtccg ggcagagcat ttgaccgcag tatacttaaa tcgtgaggag ggggagtact
    10201 atgagaacag cattctggct ggcgggcgcg acggttctgg cgctggcaaa tccggccctg
    10261 gcggcggatt ccatcaagat cggcttcgtt tctaccttca gcggcccgac cgccgtgatc
    10321 ggcaacgaca tgcgcaattc gttcgagctg gcgctcgacc acatgggccg caagatgggc
    10381 ggcctgccgg tcgaggtgat ctacgaggat gaccagcaga agcccgatgt cggcaagcag
    10441 aagaccgaaa aactggttca gtccgacaag gtggacttca tcgccggcta tatctggtcg
    10501 aacgtgctgc tggcctcgct gaaaaccgcg gtcgactcgc agaccttcct gatcagcgcc
    10561 aacgccggac catcgcagct cgccggcgag ctgtgctcgc cttacgtgtt ttcgacctcc
    10621 tggcagaacg accagacgcc gcaggcgatg ggcctctaca tgaaccagaa gggcgtcaag
    10681 tcggtgttcc tgatcggccc gaactacgcg gccggcaagg acatgctggc cggtgtgaag
    10741 agtacgttca agggcgaggt caagggcgag gaatacacgg tgtggccgag ccagctcgac
    10801 ttctcggcgg agctttccaa ggcgcgcgca tccggggcgg aatcgatctt cgtgttctat
    10861 ccaggcgcgg cgggggtgca gttcctcaat caatacgcgc aggccgggat caaggcgcaa
    10921 atcccgctat acacggcttt caccatcgac gaattgtcgc tgccgctgca gaaggacaat
    10981 gcgctcggcg tccccggcgc gcaggaatgg gtgaacgatc tgccgaacga cgagaacaag
    11041 cgcttcgtcg ccgactatcg caagaaatac cccaacctgc gtccgacctt ttacggcgcg
    11101 cagtcctatg atgccgcgca actgatcaac agcgcggtgg tggcggtgaa gggcgacacc
    11161 agcaagaagg acgcgatgaa ggcggagatg gagaaggcga atttcaaatc ggtgcgcggc
    11221 ccgttcaaat acggcaagaa ccacatcccg atccagagtt tctatctgca ggacgtggtg
    11281 aaggatgccg acggccagct ctcgctcaag acgatcgcca ccatcgtcga gaacagccag
    11341 gatcgcttcg ccgacaaatg tccgatgaag tgattggcga tcttgcccga tacagcggcg
    11401 gcgttcgcgc ggccgctgta ttcctttcgg cacatctctt gcagcccgat gacttccaga
    11461 cccacctaga ga
//
LOCUS       DATNOI010000454         7943 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_550965,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000454 DATNOI010000000
VERSION     DATNOI010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550965"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /gene="prs"
                     /locus_tag="VN968_18180"
     CDS             complement(<1..417)
                     /gene="prs"
                     /locus_tag="VN968_18180"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_028174674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HXL31198.1"
                     /translation="MSAKNGSIKLVAGNANPALAQEIANWLHLPLTKAIVRRFADMEI
                     FVEIQENVRGSDVFIIQSTSYPANDHLMELLIITDALRRASARRITAVIPYFGYARQD
                     RKVGSRSPISAKLVANIITRAGVDRVMTLDLHAGQIQ"
     gene            complement(586..1233)
                     /locus_tag="VN968_18185"
     CDS             complement(586..1233)
                     /locus_tag="VN968_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31199.1"
                     /translation="MKDTIATLFRAAAGARTAPRAALAVTLLGFACGLGGCASNGAGG
                     GSGAFAMAGSSGPTVAFESIDGPPPQIFDRMVSVLDSESKLRNLSIVSREGTSAYRVR
                     SYLSAQVSRGRTIIAWVWDVYDRDQQRALRLAGEEPAGKAGRDPWAAADELVLRKIAQ
                     AGLSGLSGMIGGTAPADAPPPATAPGKDGPAVASTEQPLPASDAFSVSALSYTTR"
     gene            complement(1381..3174)
                     /locus_tag="VN968_18190"
     CDS             complement(1381..3174)
                     /locus_tag="VN968_18190"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXL31200.1"
                     /translation="MQIDNLDDFDPGSEFEADIAIIGAGPAGITIAREFLNSPYRVLL
                     IESGLVDEHEPHMALNQVESEGEPAGEAAMNFRRIFHGPNMGSFNQQAQPYGMRVRAF
                     GGSAKYWGGRSATFDQMDFAKRDWVPFSGWPVSRESLKPYFDRAADWLNLGPNCYDEE
                     FWKLLGGEIERPPLDRSKLRSCFWQYARSRRDLTQMKSFGADFKALDSGNVRVLVNAT
                     VTQIEIDEDGKAFSHLEVSTINNVRSKVTARLCVLASGGIENARLLLASNRKRKEGLG
                     NKHDIVGRFLMDHPGTRLGHFTRGDLKKAGYLGFYTLRKDGHFRLYLHGLAFSPELQA
                     EEKLLNAAIYTLPEISPDDPIEALKRLARFKSENPFADIWALITSVGLLSKAVGLKVF
                     QSKLMPHSLQNLIINFFMVVNPSFVVREFQSKGVPHKLDCLSIHVMTEQVPDPESRIV
                     LSESRDPLGMPRAKATWKISEADRHNILRIGQLLRDELPKAGLPAPVMADWIVENRPQ
                     DAPLVDMAHATGATRMSSDPKTGVVDVNCRVHDVENLYIAGASVFPTSGHANPTLMIT
                     SLALRLADHLKVRMSTLPASVIAPREPATSA"
     gene            complement(3306..4421)
                     /locus_tag="VN968_18195"
     CDS             complement(3306..4421)
                     /locus_tag="VN968_18195"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HXL31201.1"
                     /translation="MVEEHRKIAVGLLFGGRSAEHDVSRASAANILRALNPDRYQVSL
                     IGISRDGRWLVCDAGNGAGTGAAALTIPDKAPQIALVPAGGGRFVLCDDDAAGGGAVT
                     RFDVVFPVLHGPNGEDGTVQGALELADVPFVGSAVTGSAVAMDKDVAKRLMRDAGLPV
                     VPFLAATSLSRIDYAAAVAALGTTDLFVKPANMGSSVGVSRARSEQEFGASCELAFRY
                     DHKLLVELGLNGAREIECSVLEESAGQIRASELGEIVPASKHGFYSYQAKYIDADGAA
                     LCIPAELPPKQAHRIQELAVEVFGLLGCEGLARVDFFVKGDEIFVNEVNTLPGFTSIS
                     MYPKLWEASGLSQTDLMDRLIAHALARHQRRRALSSE"
     gene            complement(4566..5336)
                     /gene="pgeF"
                     /locus_tag="VN968_18200"
     CDS             complement(4566..5336)
                     /gene="pgeF"
                     /locus_tag="VN968_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HXL31202.1"
                     /translation="MKLGSSLLSAIPGLRHAFFTREGGVSDGIYASLNGGLGSQDDPA
                     NVAENRRRMAAQMGVTPEHFLSVHQTHSPDVVVATGPSQGASRPRADAIVTRVQGLAI
                     GVTAADCGPILLVDPNARVIGAAHAGWKGALSGILESTVEAMEKLGAEPSGIVAAIGP
                     LIRQHSYEVGGEFVERFVQTDAENTLFFIPSAREGHAMFDLAGFIRMRLENAGVLMID
                     DIGVDTYSDERFYSYRRSAHLGEPDYGRHVHAIALESE"
     gene            complement(5333..6466)
                     /locus_tag="VN968_18205"
     CDS             complement(5333..6466)
                     /locus_tag="VN968_18205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXL31203.1"
                     /translation="MTEYSPLQSEIKKLIKSSGPMPVWRYMELCLTHPSHGYYISRDP
                     LGREGDFTTSPEVSQMFGELLGLWVGSVWKAMGSPSLLRLIELGPGRGTMMADALRAL
                     RVLPPLYQALSIHLVEINPVLREKQKAALSGVRIIAWHDSLDQVPNGPAVVLANEYFD
                     VLPIHQAVKRETGWHERTVEIDDGGKLAFGFAPDPMARFEVLLPPLVRAAPVGAVFEW
                     RSDTEIMKIATRMRDQGGAALIIDYGHVRSDAGDTFQAIARHSFADPLKNPGQADVTA
                     HVDFQALARAADDLGARVHGPVPQGDFLKWIGIETRAVTLMAKASHEVSEDISSALKR
                     LTDSGRGGMGSMFKVLAVSQPGLTSLAGLGDQEQAHVVRAELP"
     gene            complement(6463..7317)
                     /gene="lgt"
                     /locus_tag="VN968_18210"
     CDS             complement(6463..7317)
                     /gene="lgt"
                     /locus_tag="VN968_18210"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXL31204.1"
                     /translation="MPFLTIAFPVFDPIAIAIGPIAIRWYALAYIGGIVLGWLYARSL
                     IRNEKLWGGPAPITLVQMDDFILWVTLGVILGGRTGYVLFYNLGFFVQHPAEIFELWK
                     GGMSFHGGFLGCVAAVMLFCRKNDLPILSLGDITTAVGPIGLFLGRLANFINSELWGR
                     PADLSVPWAMVFPNGGPLPRHPSQLYEAALEGIVLFTILALMIRFGALKRPGLILGSF
                     IAIYGLARIVGEFFREPDPQLGFLWGGLTMGMLLSVPMIIAGAIIIVVAWRRKMPKQV
                     PNPIQGTP"
     gene            complement(7323..7811)
                     /locus_tag="VN968_18215"
     CDS             complement(7323..7811)
                     /locus_tag="VN968_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL31205.1"
                     /translation="MTDIRNRDYENSDAEDLNRIAVSAFGQFRDQYQDWPAMLTGLSK
                     TSALSASGEIIIVEFQKQFAGAVAYFGPNSRKAPFFDQRWPVIRMLVVDPAFRGKGLG
                     RALSTECIARAKRDGSAIIALHTSPIMTVALPMYLRMGFVKAYDAPPIFGVAYAVYTK
                     AL"
     gene            complement(7827..>7943)
                     /locus_tag="VN968_18220"
     CDS             complement(7827..>7943)
                     /locus_tag="VN968_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXL31206.1"
                     /translation="YPGTHHGFAFPKRPVYDRDAAERHWERLLALYRRNLST"
BASE COUNT         1576 a         2451 c         2418 g         1498 t
ORIGIN      
        1 ctggatttgc ccggcgtgca gatcaagcgt catcacgcga tcgacgccag cgcgggtgat
       61 gatattcgcc accagcttgg cggaaatcgg cgagcgcgaa ccaaccttgc ggtcctgccg
      121 ggcgtagccg aaatagggaa taaccgcggt gatgcgccgc gcggaagccc ggcgcagcgc
      181 gtcggtgatg atcaaaagct ccatcagatg atcgttggcc ggatacgacg tcgactggat
      241 gatgaacacg tccgagccgc ggacgttctc ctggatttcg acgaagattt ccatgtcggc
      301 gaaacggcgg acgatggcct tggtcagcgg cagatgaagc cagttggcga tttcctgcgc
      361 cagggcagga ttggcgttgc cggcgacgag cttgatggag ccgttcttgg ccgacatcga
      421 cccttctccc cgcgccttgc tgggtacgat gttcagcata tcgggatacc cacaaaaaag
      481 gcgcgtttca acgagcgagg tgatatcaag gagggggctc ggctggcaac ccgtcgtatt
      541 gggttttcct gcgcaaaatc accggtttga cgactatttg tatcgttaac gggtggtata
      601 gctcagcgcg ctgacgctga atgcatccga agcgggtaaa ggctgttcag tgctcgcaac
      661 ggcagggccg tccttgccag gtgctgtggc gggcggcggc gcgtccgccg gcgccgttcc
      721 gccgatcatt ccgctgaggc cgctcaatcc ggcctgtgcg attttccgca aaaccaattc
      781 gtcggccgcg gcccaggggt cgcgtccggc cttgccggcc ggttcctcgc cggcaagacg
      841 cagcgcacgc tgctggtcgc ggtcataaac gtcccacacc catgcgatga tggtgcggcc
      901 gcggctgacc tgggctgaaa gatagcttcg cacgcgataa gcggaggtgc cttcgcgcga
      961 gacaatcgac aggttgcgaa gcttcgactc gctgtcgagc acgctcacca ttcgatcgaa
     1021 gatctgcggc ggcgggccat cgatcgattc gaacgcaaca gtaggcccgc tgctgccggc
     1081 catggcgaaa gcgccgctac cgccgccggc gccgttgctg gcgcagccgc caagtccgca
     1141 cgcgaatccc agcagcgtca cggcgagcgc ggcgcgcggt gcggttcgcg cgcccgcggc
     1201 ggcccggaac agggttgcga tggtgtcctt catcgcgccg accgatatag ttaaaatgtg
     1261 ttaaggtcta gggcagggga gccccagcgc tttgaaaaga ttgggccgaa aaatcgccgc
     1321 ccacatgccg gccacaatct cgcctcggag agacctctac ccgatctcag cctctaccga
     1381 tcatgcggag gtggcgggct ccctgggtgc gatgacgctc gctggcagcg tactcattcg
     1441 caccttgaga tgatcggcga gccggagcgc cagcgacgtg atcatcagcg tcggattggc
     1501 atgtccgctg gtcggaaaaa ccgatgcgcc ggcgatatag agattttcga cgtcgtgaac
     1561 cctgcaattg acatcaacca cgccggtttt gggatcgctg gacatccgcg tcgcgccagt
     1621 ggcgtgcgcc atatcgacca gcggcgcgtc ttgaggacga ttctcgacga tccagtcagc
     1681 catgacaggg gcaggcagtc cggccttagg cagttcatcg cgcaacaatt gaccaatgcg
     1741 caagatgttg tgccggtcag cctcggagat tttccatgtc gcctttgccc tcggcatccc
     1801 gagcgggtcc ctggattccg atagaacaat ccggctttcg ggatcgggca cttgttcggt
     1861 catgacgtga atgctcaggc agtcgagctt gtgcgggaca cccttgcttt gaaattcacg
     1921 aactacaaaa ctcggattga ccaccatgaa gaaattgata atcagattct gcagcgaatg
     1981 tggcatgagc ttgctctgaa aaaccttcag gccaacggct ttggataaca ggccgacact
     2041 ggttatcagc gcccagatat cggcgaacgg attttcgctt ttgaacctgg cgaggcgctt
     2101 cagggcttca atcggatcat ccggggagat ttccggaagc gtgtagatgg cggcgttgag
     2161 gagcttctcc tcggcctgca gttcgggcga aaaagccagg ccatgaagat acagccggaa
     2221 atgaccgtcc ttgcgcaggg tatagaagcc cagatagccc gccttcttga ggtcgccgcg
     2281 ggtgaagtgg ccgagacgcg ttcccgggtg gtccatcagg aaacggccaa cgatgtcatg
     2341 cttgttgccg aggccttcct tgcgctttcg gttggaagcc agcaacaggc gcgcattttc
     2401 gatgccgccg ctcgccagca cgcatagcct ggcagtcacc tttgacctga cgttgttgat
     2461 ggtgctcacc tccaggtgac tgaaggcctt tccatcctcg tcgatctcga tctgtgtcac
     2521 cgtcgcattc acgagcacgc ggacgttccc ggaatcgagc gccttgaaat cggctccgaa
     2581 gcttttcatt tgcgtcagat cgcgacgaga tctggcatac tgccagaagc aggaccgaag
     2641 ttttgatcgg tcgagcggcg gcctttcgat ctcgccgccg agcagcttcc agaactcctc
     2701 atcatagcaa ttggggccga ggttgagcca gtccgccgcg cgatcaaaat acggcttcag
     2761 actctcacgg ctgaccggcc agcctgaaaa aggtacccag tcgcgctttg caaaatccat
     2821 ttggtcgaag gtcgcggacc tgccgcccca atatttcgcc gatccgccaa acgcccgcac
     2881 gcgcataccg tagggttggg cctgctggtt gaacgagccc atgttgggtc catgaaagat
     2941 ccttcgaaag ttcatcgccg cctcgccggc aggctcaccc tcgctctcga cctgattgag
     3001 ggccatgtgc ggctcatgtt cgtcgacgag gccgctttcg atcaacagca cccggtaagg
     3061 cgaattcaga aattcccgcg caattgtaat tccggctggc ccggcaccga tgatggcgat
     3121 atcggcttca aattcgctac ccgggtcgaa atcgtccaaa ttatcgattt gcatgacgta
     3181 ccaatcaggt cggcatcacg atttcgcatg agcgaaatcc aaagccacag tgttgacgcg
     3241 gatcaatgag cccttgcggc ctaccacctg atccggcggc gatcaattgc tgcatccgat
     3301 acctgtcact cggagcttag agcgcggcgc cgctgatgac gcgcaagcgc atgggcgatc
     3361 agcctatcca tcagatccgt ttgggacaat ccgctggctt cccagagctt gggatacatg
     3421 ctgatcgagg tgaatcctgg cagcgtgttt acctcgttca cgaatatttc gtcgcctttg
     3481 acgaagaagt cgactctggc caggccttcg cagccgagca aaccaaacac ctcaacggca
     3541 agctcctgaa tacggtgggc ctgcttcggg gggagttcgg caggaataca tagagcggca
     3601 ccgtcagcgt cgatgtattt tgcctgatac gaatagaagc catgcttgct cgccggcaca
     3661 atctcgccga gttcggatgc gcgaatctgg cctgcggatt cctccaggac agaacattca
     3721 atctcccgcg ctccgttcag gcccagttcg acaaggagct tatggtcgta gcggaacgcg
     3781 agttcgcatg acgcgccgaa ttcctgttcg gaccgtgcgc gcgagacccc gaccgacgat
     3841 cccatattgg caggtttgac gaataaatcg gtcgtgccga gtgcggcgac cgccgcggcg
     3901 tagtcgatcc gggacaagga ggttgctgcg aggaaaggca ccacgggaag gccggcatca
     3961 cgcatcaggc gcttggcgac atccttgtcc atcgctacgg cagaacccgt caccgctgaa
     4021 cccacgaacg gcacgtccgc caattcaagc gctccttgca cggttccgtc ttctccgttc
     4081 ggcccgtgca agaccgggaa tacgacgtcg aagcgcgtta ctgcaccgcc tcctgctgcg
     4141 tcgtcatcac acaacacgaa gcggcctccg cctgccggga ccagcgcgat ttgcggtgcc
     4201 ttgtcaggga tcgtgagcgc cgcggcgccc gttcccgcgc cattgccggc gtcgcagaca
     4261 agccagcgac catccctgga tattccgatg agagaaacct gataccggtc agggttcagg
     4321 gctcgaagga tgttggcggc cgatgccctg gagacgtcgt gttcggccga gcgtccgccg
     4381 aacaatagcc cgacggcaat cttgcgatgc tcttcaacca tcattccacc gtgtgctgct
     4441 ggtccgtagt gacggccgtt ggcctggacc cgcgtcggag atcaacgtca gcgcctacag
     4501 gatcattcca gcataaggtt ttgacaagcc tgtgttcgat gatcctgatc ggcccaaatt
     4561 tggcgtcatt cgctttccag cgcgatcgcg tgaacatgac ggccatagtc cggctcgccc
     4621 aggtgcgccg agcggcggta gctgtagaag cgctcgtcgg aataggtatc gacgccgatg
     4681 tcgtcgatca tcagcacgcc ggcgttttcg agccgcatcc ggatgaagcc ggccagatcg
     4741 aacatcgcgt ggccctcgcg cgccgaggga atgaagaaca aggtattttc cgcatcggtc
     4801 tgcacgaagc gttcgacgaa ttcgccgccc acttcgtaac tgtgctggcg gatcaggggg
     4861 ccgatggcgg cgacgatgcc gcttggttcg gcgccgagct tttccatcgc ctcgacggtg
     4921 gattccagaa tgcctgatag cgcgcctttc caccccgcat gcgccgcgcc aatcacgcgc
     4981 gcattcggat ccaccaacag gatcggtccg cagtcggcgg cggtgacgcc gatggcgaga
     5041 ccttgaacgc gggtcacgat ggcgtcggcg cgcggccgcg acgcgccttg cgacggtccg
     5101 gtggccacca cgacatcagg cgaatgggtc tgatgcacgc tgagaaaatg ctcaggcgta
     5161 acgcccatct gcgccgccat ccggcggcgg ttctccgcga cattggcggg atcgtcctgc
     5221 gagccgaggc cgccattcag gctggcgtag atgccatccg acacgccgcc ctcgcgcgta
     5281 aaaaaggcgt gacgcaggcc gggaatggcc gagagcagtg atgatccgag tttcatggca
     5341 gttccgccct gacgacgtgc gcctgctcct gatcgcccag ccctgccagc gaggtgaggc
     5401 cgggttgcga gaccgcgagc accttgaaca tcgaccccat gccgccgcga ccgctgtcgg
     5461 tcagtcgttt cagcgcgctc gaaatgtcct cggaaacctc atggctggcc ttcgccatca
     5521 gggtcaccgc acgggtttcg atgccgatcc atttcaggaa atcaccttgc ggcaccgggc
     5581 catggacgcg cgcgccgaga tcgtcggcgg cgcgcgccag cgcctgaaaa tcgacatggg
     5641 cggtgacgtc ggcctgaccg ggatttttca gcgggtcggc gaagctgtga cgggcgatcg
     5701 cctgaaaagt atcgccggcg tcgctgcgaa cgtgaccgta gtcgatgatc agcgccgcgc
     5761 cgccctggtc gcgcatccgg gttgcgatct tcatgatctc ggtgtcggac cgccactcga
     5821 acacggcgcc gaccggtgct gcgcgtacca gtggcggcag caggacttcg aagcgcgcca
     5881 tggggtcggg agcgaaaccg aaggccagct tgccgccgtc gtcgatctcg acggtgcgct
     5941 cgtgccagcc ggtctcacgc ttcaccgcct ggtggatcgg cagtacgtcg aaatattcgt
     6001 tggcgagaac gaccgcgggg ccgttgggca cctggtcgag gctgtcgtgc caggcgatga
     6061 tgcgcacgcc cgacagcgca gccttctgct tttcgcgcag caccgggttg atctcgacga
     6121 gatggatgct cagcgcctga taaagcggcg gcagcacccg cagcgcgcgc agcgcatccg
     6181 ccatcatggt gccgcggccg ggtccaagct cgatcagtcg cagcagggac ggcgacccca
     6241 tcgccttcca caccgacccc acccacaatc cgagcagttc accgaacatc tggctcactt
     6301 cgggcgaggt cgtgaaatcg ccctcgcgtc ccagcggatc gcgcgaaatg taatagccgt
     6361 gactgggatg cgtcaggcac agctccatgt aacgccagac cggcatcggc ccggacgact
     6421 tgatcaactt cttgatctcg gactggagcg gcgaatattc ggtcacggcg tcccttggat
     6481 cgggttcggt acttgcttcg gcatttttcg gcgccatgcc accacaataa tgatcgcgcc
     6541 tgcaatgatc atcggcaccg acagcagcat acccatggtc aacccgcccc acaaaaatcc
     6601 aagctgcggg tcgggctccc ggaagaactc gccgacgatg cgcgcaagac catagatcgc
     6661 aatgaagctg ccgaggatca ggcccggccg cttcagggcg ccgaagcgga tcatcagcgc
     6721 caggatcgtg aacaacacga ttccctcgag cgctgcctcg tacaactggc tgggatggcg
     6781 cggcagcggc ccgccattgg gaaataccat cgcccatggc acgcttaagt ccgccggccg
     6841 gccccacaac tcgctgttga tgaaattggc gagccgcccg agaaaaagcc cgatcggtcc
     6901 gaccgcggtg gtgatatcgc cgagcgacag gatcggaagg tcgtttttgc ggcagaacaa
     6961 catcaccgcc gccacgcagc ccaaaaagcc gccatggaac gacatgccgc ccttccacag
     7021 ttcgaaaatc tcggccggat gctggacgaa gaagccgaga ttgtagaaca gcacgtagcc
     7081 ggtgcggccg ccgagaatga ccccgagcgt cacccacagg atgaaatcat ccatttgcac
     7141 aagcgtgatc ggcgccgggc cgccccacag cttctcattc ctgatcaacg atcgcgcgta
     7201 gagccagccg agcacgatgc cgccgatata ggccagcgca taccagcgga tcgcgatcgg
     7261 tccgatcgca atggcgatcg gatcgaacac cggaaacgcg atggtgagaa agggcatctg
     7321 gctcacagcg ctttcgtgta gacggcgtag gctacgccga agatgggcgg agcatcatag
     7381 gctttgacga agcccatcct gagatacatc ggcaaggcga ccgtcatgat cggactggtg
     7441 tggagcgcga tgatggccga tccgtcgcgt ttcgcccttg cgatacattc cgtgctcaag
     7501 gcgcgcccca gtcccttccc tcgaaaggcg ggatcgacga ccagcatgcg gataacgggc
     7561 cagcgttgat cgaagaacgg ggctttccgg ctgttcggcc cgaaatacgc aacggctccc
     7621 gcgaattgct tttgaaactc gacgataatg atttctccgc tggcactgag cgccgaggtt
     7681 ttcgaaagcc cagtgagcat tgccggccag tcctggtact ggtcgcgaaa ctggccaaag
     7741 gccgagacgg caatccggtt caggtcttcc gcatccgagt tctcgtaatc cctgttcctg
     7801 atatccgtca tcggcacctg atggtttcat gtcgagagat tgcggcgata gagcgccagc
     7861 agccgttccc agtgccgctc ggcggcgtcg cgatcgtaaa ccggccgttt cggaaatgcg
     7921 aagccgtgat gggtgccggg ata
//
LOCUS       DATNOI010000455         2565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_204158,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000455 DATNOI010000000
VERSION     DATNOI010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204158"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2495)
                     /gene="gyrA"
                     /locus_tag="VN968_18225"
     CDS             complement(<1..2495)
                     /gene="gyrA"
                     /locus_tag="VN968_18225"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HXL31207.1"
                     /translation="MKRSYLDYAMSVIVARALPDARDGLKPVHRRILYSMYEQGHTPD
                     KKYVKSARVVGDVIGKYHPHGDQSIYDAMVRMAQDFSMRVPLIDGQGNFGSVDGDPPA
                     AYRYTEARLTRSATAVLGDIDKDTVDFQPNYDSSESEPRVLPAKFPNLLVNGAGGIAV
                     GMATNIPPHNLGEVVDACVALIDNPALSIDELINIIPGPDFPTGGIILGRQGIRSAYH
                     LGRGSIVMRGKVSIETMRKDREAIIISEIPYQVNKATMVERIGELHREKKIEGITDLR
                     DESDRDGFRVVIELKRDAMADVVLNQLYRFTPLQTNFAANVLALDSGRPQVMNLKDLL
                     TLFIAFREQVITRRTKFLLNKARDRAHILVGLAIAVANIDEIIRVIRTSPDPNTARET
                     LMSRDWPAKDVAAMITLIDDPRHRLAEDGTARLSFEQAKAILELRLARLTALGREEIS
                     EELDKLALEIADYLDILRSRARVQTIIKDELAAVKSEFATPRKTVIIEQEGEVEDEDL
                     IQREEMVVTVSHAGYVKRVPLSTYRAQRRGGKGRSGMQTRDEDFVSRLFVASTHTPVL
                     FFSSRGQVYKEKVWRLPVAAPNGRGKALINILPLEQGERITTIMPLPEDESSWANLDV
                     MFATTGGNVRRNKLSDFVDVRRSGIIAMKLDEDEAIVDVQICTEHDDVLLTAAGGQCI
                     RFPVTDVRVFQGRTSMGVRGIALPKNDKLISLTILRHMDADAEERAAYLRRANAVRRG
                     GVEEEAGTDSEDASGAIELGEQRYVEMSAHEQFVLTISENGYGKRSSSFEYRTTGRGG
                     KGIVAMSVNDRNGKLVASFPVEDSDQIMLVTDKG"
BASE COUNT          439 a          797 c          826 g          503 t
ORIGIN      
        1 cccttgtcgg tcaccagcat gatctggtca gagtcctcga ccgggaatga cgccaccagc
       61 ttgccgttgc ggtcattcac cgacatcgcg acgatgcctt tgccgccgcg cccggtggtg
      121 cggtattcga acgacgacga gcgcttgcca tagccgttct cgctgatggt cagcacgaac
      181 tgctcatggg cggacatttc gacatagcgc tgctcgccga gctcgatggc gccggaggcg
      241 tcctcgctgt cggtccccgc ctcctcctcg acgccgcccc gccgcaccgc attggcgcga
      301 cgcaggtagg cggcgcgctc ctcggcgtcg gcgtccatgt ggcgcagaat ggtgagcgag
      361 atcagtttgt cgttcttggg caacgcgatg ccgcgcacgc ccatcgaggt gcggccctgg
      421 aataccctga catcggtgac cgggaagcgg atgcactggc cgccggcagc cgtcagcagc
      481 acgtcgtcat gctcggtgca gatctgcacg tcgacgatcg cctcgtcctc atcgagcttc
      541 atggcgatga ttccggaacg cctgacgtcg acgaagtccg acagcttgtt gcggcggacg
      601 ttgccgccgg tggtcgcgaa catcacatcg agattggccc aggaggattc atcctcgggc
      661 agcggcatga tggtggtgat gcgctcgccc tgctccagcg gcaggatgtt gatcagcgcc
      721 ttgccgcgac cgttcggcgc cgccaccggc agccgccaga ccttttcctt gtagacctgt
      781 ccgcgcgacg agaagaacag caccggcgta tgggtcgagg ccacgaacag gcggctgacg
      841 aaatcctcgt cgcgggtctg catgcccgaa cggcccttgc cgccgcgccg ttgcgcgcga
      901 taggtcgaca gcggcacccg cttgacgtag ccggcatgcg agaccgtgac caccatttcc
      961 tcgcgctgga tcaggtcctc gtcctcgacc tcgccttcct gctcgatgat gacggtcttg
     1021 cgcggcgtcg cgaattccga cttcaccgcg gcgagttcgt ccttgatgat ggtctggacg
     1081 cgggcgcgcg agcgcaggat gtcgaggtaa tcggcgattt ccagcgcgag cttgtcgagt
     1141 tcctcggata tttcctcgcg gccgagtgcc gtcaggcgcg ccagacgcag ctcgagaatg
     1201 gctttggcct gctcgaacga cagccgtgcg gtgccgtctt cggcgagccg atgccgggga
     1261 tcgtcgatca gcgtgatcat cgccgccacg tccttggccg gccagtcgcg tgacatcagg
     1321 gtttcacgcg cggtgttggg atcgggcgag gtccggatca cgcgaatgat ctcgtcgata
     1381 ttggccaccg caatcgcgag accgaccagg atatgggcgc ggtcgcgggc cttgttgagc
     1441 aggaacttgg tgcggcgggt gatgacctgc tcgcggaagg cgatgaacag tgtcagcaga
     1501 tccttcaggt tcatcacctg cggccggccc gaatcgagcg ccaacacatt ggcggcaaaa
     1561 ttcgtttgca gcggcgtgaa gcgatagagc tgattcaaca ccacgtccgc catcgcatcg
     1621 cgcttcagtt cgataacgac gcggaaacca tcgcggtcgg attcgtcgcg cagatcggta
     1681 atgccctcga tctttttctc gcgatggagt tcgccgatgc gctcgaccat ggtggccttg
     1741 ttcacctggt agggaatttc cgaaatgatg atcgcttcac gatccttgcg catggtctcg
     1801 atcgacacct tgccgcgcat cacgatcgag ccgcggccga gatgataggc tgatcggatg
     1861 ccctggcgtc cgaggatgat gccgccggtt ggaaaatccg gtcctggaat gatattgatg
     1921 agctcgtcga tgctgagcgc gggattgtcg atcagcgcca cgcaggcgtc gaccacctcg
     1981 ccgagattgt gcggcggaat gttggtggcc atgccgaccg cgatgccgcc ggcgccgttg
     2041 accagcaggt tcgggaattt ggccggaaga acccgcggct cgctctcgga actgtcgtaa
     2101 ttgggctgga aatcgacggt gtccttgtcg atatccccga gaaccgccgt tgcggatcgg
     2161 gtgagacgcg cttccgtata ccgataagcc gccggcggat caccatcgac cgagccgaaa
     2221 ttgccttggc cgtcgatcag cggcacgcgc atggagaaat cctgcgccat gcggaccatc
     2281 gcatcgtaaa tcgactggtc gccgtgtgga tggtacttac cgatcacgtc gccgacgacg
     2341 cgcgcggact tgacgtactt cttgtcgggc gtgtgccctt gctcatacat cgaatacaga
     2401 atgcggcgat gcaccggctt gaggccgtcg cgcgcgtcgg gcagcgcacg cgccacgatc
     2461 acgctcatgg cgtaatcgag gtaggagcgt ttcatctcgt cgaggatgga tacgggacga
     2521 atatccgagg gcgccggcgg ctctcccggc ttcgtatctt caggg
//
LOCUS       DATNOI010000456         9094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2164245,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000456 DATNOI010000000
VERSION     DATNOI010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9094
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164245"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VN968_18230"
     CDS             complement(<1..437)
                     /locus_tag="VN968_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL31208.1"
                     /translation="MPTLNINGRNMSVEAANDTPLLWAIREQLQMTGTKFGCGAGLCG
                     ACTVHVNGEAVRSCQTSLSDVAGKKITTIEGLSAKGDHPLQKAWIAEQVPQCGYCQSG
                     QIMQAAALLSKNANPTKDEVVAHMDGNLCRCMTYSRIQKAIMRA"
     gene            787..1581
                     /locus_tag="VN968_18235"
     CDS             787..1581
                     /locus_tag="VN968_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL31209.1"
                     /translation="MPKIDRDGIQIYYEVHGSGPPLLLTHGYSSTSQMWQGQIEALSK
                     HHKLVLWDMRGHGRSDYPGDASAYSEALTVADIAALLDEVGAISAIVGGLSLGGYMSL
                     AFYRAHPARVRALLIIDTGPGFKKDDAREAWNKRARDTSDRFEREGLEALKSGSRERS
                     TAGHRDASGLARAARGMLTQRDARVIESLPDIKVPSLVVVGADDTPFLAASDYMAAKI
                     PGAKKAVIPAAGHAVNIDQPQAFIDAVLPFLDSLPQSAVAERMIPS"
     gene            1578..1949
                     /locus_tag="VN968_18240"
     CDS             1578..1949
                     /locus_tag="VN968_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31210.1"
                     /translation="MNRLALIAVSVAGLGAAGLGAAGIDAAAARTLAPAGGSNAPPFT
                     ATLSNNTPLVFGMDAEAAARALGTPLNYISGRPGDETLLAFRNVGGSGLFFHKDRLYL
                     QFRKGRLTGWKGDWGDNWMWQ"
     gene            2024..2698
                     /locus_tag="VN968_18245"
     CDS             2024..2698
                     /locus_tag="VN968_18245"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXL31211.1"
                     /translation="MGQDIKLTASDNFQLGGYRADPATAPRAAVVVIQEIFGVNHHIR
                     AVCDRLAGEGYVAIAPAIFDRVEPNFTSGYSPDEIAVARKFVANPDWAAMLRDTQAAI
                     DAVSPIGPVGIIGFCLGGSIAYAAATKLSGLSAAVGYYGGAIVRFADDKPKAPTQLHF
                     GEKDAGIPLADVETIGAKRPEVEIHIYPGAQHGFHCDERASYDKASADIAWPRSLGFF
                     AQHLKK"
     gene            complement(2873..3529)
                     /gene="msrA"
                     /locus_tag="VN968_18250"
     CDS             complement(2873..3529)
                     /gene="msrA"
                     /locus_tag="VN968_18250"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXL31212.1"
                     /translation="MLFMRKTTALPSAAEALPGRANPIPTAASHFVNGRPLQPPYPAG
                     LEQAVFGLGCFWGAERKFWQLGDGIYTTAVGYAGGRTRNPSYEEVCSGLTCHTEAVLV
                     VFDPNKVSYDQLLKTFWESHNPTQGMRQGNDVGTQYRSAIYTFNDAQRQAADASKSAY
                     QRALAAKGLGAITTEIAPSGEFYFAEEYHQQYLAKNPAGYCGLGGTGVSCPVGAGVSA
                     "
     gene            complement(3641..3955)
                     /locus_tag="VN968_18255"
     CDS             complement(3641..3955)
                     /locus_tag="VN968_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31213.1"
                     /translation="MIRGFFRLVGLLLLAGGFIFMVYDGARWVADQTLRFTRFGEFWN
                     DIHQSSQQAFRAWVEATAPWLWTSVKIILDQPVFAVMGVLGILLMLLFRPRKPLIGYS
                     RN"
     gene            4312..4578
                     /gene="rpsT"
                     /locus_tag="VN968_18260"
     CDS             4312..4578
                     /gene="rpsT"
                     /locus_tag="VN968_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXL31214.1"
                     /translation="MANTTSAKKATRKIARRTIINKSRRTQMRGAVRIVEEAIKSGDR
                     DAALKAMTRAEPELMQAAQRNIIHKNNASRKVSRLAHQIAKLAK"
     gene            5811..7241
                     /gene="dnaA"
                     /locus_tag="VN968_18265"
     CDS             5811..7241
                     /gene="dnaA"
                     /locus_tag="VN968_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HXL31215.1"
                     /translation="MTNIEQDRWSRVKGRLRSSVGEDVYSSWFARMDLEGVQDESVRL
                     SVPTRFLKSWIQAHYAERVLTCWQAEMPEVHRIDLTVRTAMRCAAPAREALAPLEQRR
                     AERSDGRPAPELRATAIAPVSANHDALGGSPLDPRLTFGSFVIGRSNTLAHAAARQVA
                     EGRRGDSVMFNPLYIHAGVGLGKTHLLQAVTWAGNSGSERKVLYLTAEKFMYGFVAAL
                     KTQTALAFKEALRGIDVLVIDDLQFLQGKSTQAEFCHTLNALIDAGRQVVIAADRPPS
                     DLESLDDRVRSRLAGGLVVEMSSLGEELRLGILKSRVAAARAHHASFDVPAPVLDYLA
                     RTITHNGRDLEGAINRLLAHSKLNAQPVTLEMAEREVRDLIRPQEPKRIKIEDIQRVV
                     ARQYNVSRSDLLSSRRTANVVRPRQVAMYLAKTLTLRSLPEIGRRFGGRDHTTVLHAV
                     RKIEALVTRDVALSEEVESLKRQLQE"
     gene            7593..8711
                     /gene="dnaN"
                     /locus_tag="VN968_18270"
     CDS             7593..8711
                     /gene="dnaN"
                     /locus_tag="VN968_18270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXL31216.1"
                     /translation="MKVTVERAQLLKSLGHVHRVVERRNTIPILGNVLIRAESAKLSL
                     KATDLDLEVTETLAAESATGGSTTVPAHMFYDIVRKLPDGAQIVLEGDGDRSVLAIRA
                     GRSRFTLQTLPENDFPDLAAGDMTHSFTLPASDMKRLIDRTQFAISTEETRYYLNGIY
                     LHSAGSAKEPTLRAVATDGHRLAQFDLPLPKGASGTPGVIVPRKTVGEVLRLIEDNEA
                     EVGIELSQGKIRFTLGNVVLTSKLIDGTFPDYGRVIPQNNDKELIVDKKDFEAAVDRV
                     STISSERGRAVKLALSAGKLVLSVTNPDSGSATEELEVEYASEALDIGFNSRYLLDIA
                     AQIEGEVAVLRLADPGSPTLVQDKDSKGALYVLMPMRV"
     gene            8811..9053
                     /locus_tag="VN968_18275"
     CDS             8811..9053
                     /locus_tag="VN968_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXL31217.1"
                     /translation="MYNGVTNNLVRRVYEHREGLAESFTKRYGIKTLVYFEPHETIAT
                     PLQREKNIKHWSREWKIDLIVAGNPDWCDLYEEIVR"
BASE COUNT         1822 a         2818 c         2790 g         1664 t
ORIGIN      
        1 gcacgcatga tcgccttttg gatgcgcgaa taggtcatgc agcggcaaag attgccgtcc
       61 atgtgcgcga ccacttcgtc cttggtcgga ttggcgttct tggagagcaa tgccgccgcc
      121 tgcatgatct gcccggattg acagtagccg cattgcggaa cctgctcggc gatccacgcc
      181 ttctgcagcg gatggtcgcc cttggcgctg agcccttcga tggtggtgat tttcttgccg
      241 gcgacgtcgc tgagcgaagt ctggcaggat cgcaccgctt caccgttgac gtggaccgtg
      301 caggcgccac acaggccggc gccgcagccg aatttcgtac cggtcatctg caattgttcg
      361 cggatggccc aaagcaacgg cgtatcgttc gccgcctcga cggacatatt ccgcccattg
      421 atgttgagag taggcatggt ctctccccct gccggtgcat gatgccgctt acggcggcga
      481 ttcacgtttg acgaatggtc tggcacccac cgggccgatg ccgaccttgc gcggcagaat
      541 gatcgcgttc caatccagag gcaatccaaa ttggaatcgg tcgaatagaa aatggtctga
      601 aatgcgcttt gtgcgctgcg gcaacagacc ccgccggaaa atgggcgatg gacttgctcc
      661 cacgccgtgc ggcgccggcg attgccgctt gcgaagttgg gcagttccca gaagcgaacg
      721 gatcgcgcta gaataccgtt gcacggcagc ttcacgaagc cgccggggaa tcgccgggaa
      781 actgcaatgc caaaaatcga ccgcgacggg atccagatct attatgaggt ccacggcagc
      841 ggaccgccgc tgctgctgac ccacggctat tcgtcgacat cgcagatgtg gcaaggccag
      901 atcgaggcct tatcgaaaca ccacaaactc gtgttgtggg acatgcgcgg ccacggccgg
      961 tccgattacc ccggcgacgc gtcggcctat agtgaagcgc tgacggtggc ggacatcgca
     1021 gccctgcttg acgaggtcgg ggccatcagc gccatcgtcg gcgggctttc gctcggaggc
     1081 tacatgtcgc tggcgtttta tcgcgcgcat ccggcgcgcg tgcgggcgct gttgatcatc
     1141 gataccgggc cgggcttcaa gaaggacgac gcccgcgagg cctggaacaa acgcgcgcgc
     1201 gataccagtg accgcttcga gcgcgaaggc ctggaagctt tgaaatccgg cagccgcgag
     1261 cgctcgacgg cgggccatcg cgatgcgtcg ggcctggcgc gcgccgcgcg cggcatgctg
     1321 acccagcgcg atgcccgcgt gatcgaatcc ttgcccgaca tcaaggtacc ttcgctggtg
     1381 gtggtcggcg ccgacgacac gccgttcctc gcggcctcgg actatatggc ggcgaaaatt
     1441 ccgggcgcga aaaaggcggt gatcccggca gcgggccacg ccgtcaacat cgaccagccg
     1501 caggcgttca tcgacgcggt gttaccgttc ctcgatagcc tgccacaaag cgccgtcgcc
     1561 gaaaggatga tcccgtcatg aacaggctcg cgctgatcgc ggtttccgtc gcgggtcttg
     1621 gagccgcagg ccttggagcc gcaggcatcg acgccgcggc cgcccggaca ttggcgccgg
     1681 cagggggttc gaatgcccca cccttcaccg cgacgctgtc gaacaatacg ccgctcgtgt
     1741 tcgggatgga cgcggaagct gccgcgcggg ccttggggac accattgaac tacatcagcg
     1801 gccgcccggg cgacgaaacc ttgctggcct tccgcaacgt cggcggcagc gggttgttct
     1861 tccacaagga ccggctctat ctgcaattcc gcaagggccg actgaccggc tggaagggcg
     1921 actggggcga caactggatg tggcaatagc agccggcctg acgcttggct ttcgctttca
     1981 aaactctccg caaaagatca actctcaagc aaatggatga ccggtgggac aagacatcaa
     2041 gctgacggct tcggacaatt ttcaactggg cggctatcgt gccgaccccg ccaccgcgcc
     2101 cagggcggcg gtggtggtga tccaggaaat ctttggcgtc aatcatcaca tccgcgcggt
     2161 ctgcgaccgt ctcgcaggcg agggctatgt cgccatcgcg cctgcgatct tcgaccgcgt
     2221 cgagcccaat ttcacctccg gctattcgcc cgatgagatc gcggtggcgc gcaagttcgt
     2281 cgccaatccg gactgggcgg cgatgctgcg cgacacccag gccgccatcg atgcggtcag
     2341 ccctatcggc ccggtcggca tcatcggttt ctgcctcggc ggcagcatcg cctatgccgc
     2401 cgcgacaaaa ctgtcggggc tgtcggcggc ggtcggctat tacggcggcg ccatcgttcg
     2461 tttcgccgac gacaagccga aagcgccgac gcagttgcat ttcggagaaa aggatgcggg
     2521 cattccgctc gccgatgtcg agaccatcgg cgcaaaacgg cccgaggtcg agatccacat
     2581 ctatccgggg gcgcagcacg gctttcattg cgacgagcgg gcaagctacg acaaggccag
     2641 tgccgatatc gcctggccgc gcagcctcgg cttcttcgcg cagcatttga agaagtgaga
     2701 gctcgtcatg cgcgggcttg acccgcgcat ccatcaaaaa gaagctcttt tgaagaagaa
     2761 gatggattgc cgggtcaaag cccggcaatg acgatgagaa tgtgcctcac aatcagcgct
     2821 cgccgaccaa gacagtatcg cccgcgccaa ccgcagtgcc gctttaaggc gatcaggcgc
     2881 tgacgcccgc gccgaccgga cacgacaccc cggtgccgcc aagcccgcaa tagccggccg
     2941 gattcttcgc cagatactgc tggtgatact cctcggcgaa atagaattcg cctgatggcg
     3001 cgatctccgt ggtgatggcg ccgaggccct ttgccgccaa cgccctttga taggccgact
     3061 tggatgcatc cgcggcttgg cgttgcgcgt cattgaaggt gtagatcgcg gatcgatact
     3121 gggtgccgac gtcattgccc tgacgcatgc cctgcgtcgg attgtgactt tcccaaaacg
     3181 tcttcagcaa ttgatcgtac gaaaccttgt tcggatcgaa caccaccagc accgcttcgg
     3241 tatgacaggt caggcccgaa caaacctcct cgtaactggg gtttcgggta cgaccgccgg
     3301 cgtaacccac cgcggtggtg tagataccgt cgccgagctg ccagaatttg cgctccgcac
     3361 cccagaagca gccgagcccg aacacggcct gctccagacc ggcgggataa ggtggctgca
     3421 acgggcggcc gttgacgaaa tggctggcgg cggtggggat cggattggcg cggcccggca
     3481 gcgcctcggc tgcgctcggc aacgcggtgg tcttgcgcat gaacaacatg ggctttctcc
     3541 cggcgggaac agatgtcact ttattatacg tatctacgca ggtcacggca gcccggttgc
     3601 gccgggcagc cgaacataaa gccggcaccg ccctatccgg tcagttccgc gaatagccga
     3661 tcagcggctt gcgcggccgg aacagcagca tcagcaagat acccaacacg cccatcacgg
     3721 caaaaaccgg ttggtccagg ataattttga cgctggtcca gagccagggc gcggtggcct
     3781 ccacccaggc ccggaacgcc tgctggctgg attgatggat atcgttccag aattcgccaa
     3841 aacgggtaaa gcgcaaggtc tgatcggcga cccagcgcgc gccgtcatag accatgaaga
     3901 tgaatccgcc ggccagcagc agcagcccca caagtcggaa aaaaccgcgg atcatgcgtc
     3961 accctatgtc tcttgcgccc gaaaacgccg tcagccatta ctggcacggc gcgggaaatt
     4021 caaccacatc agcatcttat cagcgccttc cgatcgggcc tccgggcttt gggggcccgg
     4081 aaagcgttga cgctgcaatt tgcgccctct ataagaccgc caatggtggc ggacgcaatc
     4141 ctgccgccgc tgttctttga gcggcgccgt cgggtgagcg atccatgctc cgagaatggc
     4201 attctcgtgg aacacccgga aacataccag cgtcgatcac gcagttgaac gggcggagcg
     4261 ctcgagccta agcacccgcg atcggtcacc gtcgaaggat atttgaaagc catggccaat
     4321 acgacctccg ccaagaaggc gacgcgcaag attgcccgcc gcaccattat caacaagtcc
     4381 cgtcgtaccc agatgcgcgg cgcggttcgc attgtcgaag aggcgatcaa gagcggcgac
     4441 cgcgatgcgg cgctgaaggc gatgacgcgc gcggaaccgg aacttatgca ggccgcgcag
     4501 cgcaacatca tccataagaa caatgcgagc cgaaaggtct cgcggctcgc gcatcagatc
     4561 gcaaagctcg cgaaatgaac tgatcaagga aatattcgtc aaggaagccc ggcattgccg
     4621 ggcttcttct gtttcgccgg cctctgcacg gccggtctcg gcctcccgag acgccgattg
     4681 cccccggatt tttacggaga tttttccgcc gtatgtatga aggcgggttt acaaagcccg
     4741 cgatgaccgc gacgatggtg ctgccggtgt ttgatcgcgc aggtttttga aatagcaatc
     4801 atgatcgcgc gacggcgcac cgagatcgct cagggttacc ctgttccgac gagcaaaaaa
     4861 aaacgcgctt gcgtagtcac ttatcggcat agcggcgacc agttgtccga aaattccgat
     4921 ctgccccgct caaacgtaaa cgatttatga atttattttg ggcagccgtg agaaatgtga
     4981 cagcggcgcg gcagttggat tctccgcaca gattcaaaac cttggctctt tgcgcgaaaa
     5041 ccgctgcctg tttttgcgaa tgcgaagcca attctaccgc cacgaccccg gaatcatttt
     5101 cgttgcgggc aatagggcat catcgtatct cttagaacgt tcgtgacgcc tccggctcca
     5161 aagaccagcg cggttcaagg aacggggcgg acgtgcaaat cggcggcggt atgcgcgttg
     5221 gcgacgctct ccaagcgatt gtcggatata aactcatacc aatatgagga cggtagttct
     5281 gtgtcgaaat ttctcaagcg ggggcattgc gcctcgctcg ctttgggcaa gagatattcg
     5341 cacaagccga ccgcaatcgc gccgaaggcg aagcaggcgg gcgtgttgta acggggctta
     5401 accaacgcgg cggcgaagcg gctgaacgat tttgatgttt ggacgcgatc gactgaattt
     5461 gaaactcacg tgatatgaca acttgctgtg agcaatcacg gcgggatggg gaggttgcat
     5521 gctttatgga atcaacgcgg tattcgatca aataccattt tccgacgaca ctgtatggaa
     5581 ccgcctggat gccgcctgcg cgcatcctcg cacgcgaact ccggacactc cgttgagtac
     5641 gcgctgacct cgcggaccct gcttcttcca gcctgatgat tttgcccacg gcatctcgcc
     5701 gaacggctgg tcgttggctg aagacgaagc cccgatcgag gtcgttctgt gcggaacacc
     5761 cgcacggcgg ttcgcaggca atatcactcc atcagaaaag tcgtaaggca atgacaaata
     5821 tcgaacagga tcgctggtca cgcgtgaagg gtcggctgcg ctcaagcgta ggcgaagacg
     5881 tctattcaag ctggtttgcg cgaatggatc tggagggcgt gcaagacgaa agcgtccgcc
     5941 tttcggttcc gacccggttc ctcaagagct ggatccaggc acattatgcc gagcgtgtcc
     6001 tcacctgctg gcaggccgag atgccggaag tgcaccggat cgacctcacc gtgcgcacgg
     6061 cgatgcgatg tgcggcgccg gcgagagagg ccctcgcgcc gctcgagcaa cggcgcgccg
     6121 agcgatccga tggtcgtccc gcgcctgagc tgcgcgcgac cgcgatcgcg ccggtttccg
     6181 ccaaccatga cgcgctcggc ggttcgccgc tcgatccgcg cctgaccttc ggaagttttg
     6241 tgatcggccg ttccaacacg ctggcacacg ccgcggcgcg ccaggttgcg gaagggcggc
     6301 gcggcgatag cgtgatgttc aatccgctct acatccacgc cggcgtcgga ctcggcaaaa
     6361 cccatctgct gcaagccgtg acatgggccg gcaattccgg cagcgaacgc aaggtgcttt
     6421 accttacggc cgaaaaattc atgtacggct tcgtcgcggc gctcaagacc cagaccgcgc
     6481 tcgcattcaa ggaagccttg cgcggcatcg acgtgctggt gattgacgat ctgcaattcc
     6541 tgcagggcaa atcgacccag gctgaattct gtcatacgct gaatgcgctg atcgatgccg
     6601 gtcgccaggt ggtgatcgcc gccgatcgtc cgccttccga cctggagagc ctcgacgatc
     6661 gcgtgcgttc gcgtctcgcg ggcggactgg tggtcgagat gagttcgctc ggcgaggaat
     6721 tacggctcgg aatcctcaaa tcgcgggtcg cagccgcgcg ggcacatcat gcgagcttcg
     6781 atgtgccggc accggtgctg gattatctgg ccagaaccat cacccacaat ggccgcgacc
     6841 ttgaaggcgc catcaaccgc ctgctcgcgc acagcaagct caacgcgcag ccagtgacac
     6901 tggaaatggc cgaacgcgaa gtgcgcgacc tgatccgtcc gcaggagccc aaacgcatca
     6961 agatcgagga cattcagcgg gtggtggccc ggcagtacaa tgtcagccgc tccgacctgc
     7021 tgtcgtcgcg gcgtacggcg aatgtcgttc ggccacgcca ggtcgcgatg tatctggcca
     7081 agacgctgac gctgcgctcg ctgcccgaaa tcggccgccg gttcggggga cgcgaccaca
     7141 ccacggtcct gcatgccgtg cgcaagatcg aggccctggt cacaagggat gtggcgctct
     7201 ccgaagaggt cgaatcgctg aaacgtcaac tgcaggagtg aacgtccctg caacaaggcg
     7261 ccgtctcgga ttggtcgtcc cggcggaacg ccgggatcca tacgccgtga catctcgttt
     7321 cggcagtggg gcggatgcct tctgcggcca ttaacgccag gggttatggg tcccggcgtt
     7381 tcgccgggac cacctgcaaa ggccggattt cccgcaaaac gtggggaacc ggctcacgca
     7441 tcccttgcgc ttgcgcgcca tccgcgccac cttgcggtcc ccctgcgggt ttgatcaaat
     7501 ccagctatga tcgggctttt cgggtctcaa acgccgcggc gcgccttccg ggccgcgcgg
     7561 cttttccatc ttaggatcag gcgggtattg caatgaaggt taccgtcgaa cgcgcgcaac
     7621 tcctgaaatc gctgggccac gtccatcgcg tggtcgagcg ccgcaacacc attccgatcc
     7681 tcggcaacgt gctgatccgc gccgaaagcg ccaagctgtc gctgaaggcc accgaccttg
     7741 acctcgaggt gacggaaacg ctggcggcgg aaagcgcaac cggcggttcg accacggtgc
     7801 cggcgcacat gttctacgac atcgtgcgca agctgcccga tggcgcgcag atcgtgctgg
     7861 aaggcgatgg cgaccgctcg gtgctggcga tccgcgccgg ccggtcgcgc ttcacgctgc
     7921 agaccctgcc cgaaaacgat ttccccgatc tcgccgccgg cgacatgacg cattcgttca
     7981 cgctgcccgc ctccgacatg aagcggttga tcgaccgcac ccagtttgcg atctcgaccg
     8041 aggaaacccg ttactatctc aacggcatct atctgcactc cgccggcagc gccaaggagc
     8101 cgaccctgcg cgcggtcgcg accgacgggc atcgtctcgc gcagttcgat ctgcctttgc
     8161 ccaaaggcgc atccggcacg cccggcgtca tcgtcccgcg caaaaccgtc ggcgaagtgc
     8221 tgcggttgat cgaggacaac gaggccgaag tcgggatcga actgtcgcag ggcaagatcc
     8281 gcttcacgct cggcaacgtg gtgctgacct cgaaactgat cgacggcacg tttcccgact
     8341 acggccgcgt catcccgcaa aacaacgaca aggaactcat cgtcgacaag aaggatttcg
     8401 aggccgcggt cgaccgcgtc tcgaccatct cgagcgagcg cggccgcgcg gtgaagctgg
     8461 cgttgtccgc gggcaagctg gtgctgtcgg tgaccaatcc ggattccggc agcgcgaccg
     8521 aagagctgga ggtcgaatac gcttccgagg cgctcgatat cggattcaat tcgcgctatc
     8581 tgctggacat cgccgcccag atcgagggcg aagtcgcggt gctgcgactg gccgacccgg
     8641 gttcgccgac gctggtgcag gacaaggatt ccaaaggcgc gctctacgtg ctgatgccga
     8701 tgcgggtgta gccaacgttt tcaccatcgt cattgccggg cttgacccgg caatccatga
     8761 acgaaatgac ccattgggta tacattctag ccagttagcc cggtggaacg ctgtacaacg
     8821 gcgtgacgaa caatctggtg cggcgtgttt acgagcatcg ggaggggcta gctgaaagtt
     8881 tcaccaaacg ctacggcatc aaaacgctgg tatattttga accacacgaa acaatagcga
     8941 ccccgctcca acgcgagaag aacatcaagc attggtcgcg cgagtggaag attgatctga
     9001 ttgtcgcggg caatccggat tggtgcgatc tatatgaaga gattgtccgc tagcgcggac
     9061 gatggattgc cgggtcaagc cggcaatgac aact
//
LOCUS       DATNOI010000457         2264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1624341,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000457 DATNOI010000000
VERSION     DATNOI010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624341"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VN968_18280"
     CDS             complement(<1..806)
                     /locus_tag="VN968_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXL31218.1"
                     /translation="MPSSSPISSVNDFVVRFANVNGSGSASANELFARSVLRMGIPVA
                     PRNIFPSNIQGLPTWYEVRISEAGHLGARGGTDLMVAMNPQTWDRDVAGIEPGGYLLY
                     DCTKPLPTSKFRDDITVIGVPLTAICNREYTDARQRQLFKNIVYVGALCALLDMDVEA
                     TERLVAEQFRSKEKLIQANLHALHLGRDWALENLQCPIGLRLRHAKTVGDRIFIEGNS
                     AAALGAVYGGATVCAWYPITPSSSLADAFTGHCRRLRVDPVTKKNKVAIV"
     gene            complement(776..>2264)
                     /locus_tag="VN968_18285"
     CDS             complement(776..>2264)
                     /locus_tag="VN968_18285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL31219.1"
                     /translation="KNGKRIALVGGGPASLTVARDLAPLGYHCVVFDQDPKAGGMIRT
                     QIPRFRLPESIIDEECDYILNLGIEFHGGKRIDSLKALLAEGWDAIFVGSGAPRGRDL
                     EIPGRKEAAANIHIGIDWLSSVSFGHVSKIGKRVIVLGGGNTAMDCCRSSRRLGGEDV
                     KVIVRSGFQEMKASPWEKEDAMHEDIPILNFLVPKEFTHENGRLTGVLFEKVKAEYDA
                     KGRRNLVPTGEPHQHMACDDVLVAVGQENAFPWIERDVGIEFDKWDMPKVDPKTMAST
                     HPRVFFGGDSAFGPKNIIWAVAHGHDAALSIDKLCSGADINDRPQSMVEIVSQKMGIH
                     EWSYDNDIAPDRRYRVPLRDKVVALRDIKAEVELGYDVNLALKEAHRCLNCDVQTVFT
                     SSLCIECDACVDICPMDCITFTANGEEADLRTRLKAPAQNLTQDLYVANDLKTGRVMV
                     KDEDVCLHCGLCAERCPTGAWDMKKYLIDMTHANDQCRVAARSAA"
BASE COUNT          376 a          702 c          753 g          433 t
ORIGIN      
        1 tgcacgatcg cgaccttgtt cttcttggtc actggatcga cccgcagccg cctgcaatgg
       61 ccggtgaagg catccgccag cgaggatgac ggcgtgatcg gataccaagc gcacacggtc
      121 gcgccgccgt agaccgcgcc cagcgcggcg gccgagttgc cttcgatgaa gatgcgatcc
      181 ccgaccgtct tggcgtggcg caagcgcagc ccgatcgggc actgcaggtt ctcgagcgcc
      241 cagtcgcgcc cgagatggag cgcatggagg tttgcttgga tgagtttttc cttcgagcgg
      301 aactgctcgg cgacgagccg ctcggtcgcc tcgacgtcca tgtccagcag cgcacaaagc
      361 gccccgacat agacgatgtt cttgaacagc tggcgctggc gcgcatcggt gtattcgcga
      421 ttgcagatcg cggtcaacgg cactccgatg acggtgatgt cgtcgcggaa cttggacgtc
      481 ggcagcggct tggtgcaatc gtagagcaga tatccgccgg gctcgatccc cgccacgtcc
      541 ctgtcccagg tctgcgggtt catggccacc atgagatcgg tgccgccgcg ggcgccgagg
      601 tgtccggctt ccgagatgcg cacctcgtac caggtcggca ggccctggat gttggacggg
      661 aaaatgttgc gcggcgcgac cggaataccc atgcgcagca ccgagcgcgc gaacagctcg
      721 ttggcgctgg cggagcccga gccgttgacg ttggcgaacc ggacgacgaa gtcgtttacg
      781 ctgctgatcg ggctgctact cggcattggt cattcgcgtg agtcatgtcg atgaggtatt
      841 ttttcatgtc ccaggcgcca gtcggacagc gttcggcgca caacccgcag tgcaggcaga
      901 catcctcgtc cttgaccatg acgcggccgg tcttgagatc gttggcgacg tagaggtcct
      961 gggtgagatt ctgggcgggt gccttgaggc gggtccgcag gtcggcttcc tcgccgtttg
     1021 ccgtaaacgt gatgcagtcc atcgggcaaa tgtcgacgca ggcgtcgcac tcgatgcaca
     1081 gcgaggaggt gaaaaccgtc tgcacgtcgc aattgaggca gcggtgcgcc tccttgaggg
     1141 cgagattgac gtcgtagccc agctcgacct cggccttgat gtcgcgcagc gccaccacct
     1201 tgtcgcgcag cggcacgcga tagcgccggt cgggcgcgat gtcgttgtcg tagctccact
     1261 cgtgaatgcc catcttctgg ctgacgatct cgaccatgga ctggggacga tcgttgatat
     1321 cggcgccgga gcacagcttg tcgatcgaca gcgcggcgtc gtggccgtgg gcgaccgccc
     1381 agatgatgtt cttcgggccg aaggcggagt cgccgccgaa gaacacccgc ggatgggtcg
     1441 aggccatggt cttgggatcg accttcggca tatcccattt gtcgaactcg atgccgacgt
     1501 cgcgctcgat ccaggggaaa gcgttctcct ggccgaccgc gaccagcacg tcgtcgcaag
     1561 ccatgtgttg atgcggttcg ccggtcggaa cgaggttgcg ccggcccttg gcgtcgtatt
     1621 cggccttcac cttctcgaac agcacgccgg tgaggcggcc gttctcgtgg gtgaactctt
     1681 tcggcacgag gaaattcagg atcgggatgt cttcgtgcat ggcgtcctcc ttttcccagg
     1741 gagacgcctt catttcctgg aagcccgagc gcacgatcac tttcacatcc tcgccgccga
     1801 gacggcgcga cgagcggcag caatccatgg cggtgttgcc gccgccgagc acgattacgc
     1861 gcttgccgat tttgctgacg tggccgaagg agaccgatga gagccagtcg atgccgatat
     1921 ggatgttcgc cgccgcttcc ttgcggccgg gaatctcgag atcgcggccc cgcggcgcgc
     1981 ccgagccgac gaagatcgca tcccagcctt ccgccagcag cgccttgagg ctgtcgatgc
     2041 gcttgccgcc gtgaaattcg atgccgaggt tgaggatata gtcgcattcc tcgtcgatga
     2101 tcgattccgg cagacggaag cgcggaatct gcgtgcggat catgccgccg gccttggggt
     2161 cctggtcgaa caccacgcag tgatagccga gcggcgccag gtcgcgcgcg accgtgagcg
     2221 aggcggggcc gccgcccacg agcgcgatgc gcttgccgtt cttg
//
LOCUS       DATNOI010000458         5246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_905577,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000458 DATNOI010000000
VERSION     DATNOI010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905577"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VN968_18290"
     CDS             <1..324
                     /locus_tag="VN968_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041031.1"
                     /note="with UgpACE is involved in the uptake of
                     glycerol-3-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HXL31220.1"
                     /translation="KPEIQAAWHQNTGYLPITRAAFDLTRAQGFYERNPGTAISIEQI
                     TLKTPTENSRGVRLGSFVLIRDVIDDELEQAFSGKKSAQAALDSAVERGNRLLRQFER
                     ANPDR"
     gene            337..1287
                     /locus_tag="VN968_18295"
     CDS             337..1287
                     /locus_tag="VN968_18295"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase/choline
                     kinase family protein"
                     /protein_id="HXL31221.1"
                     /translation="MHSLPSLSNFEALQAWRADASQCLSAALDIARSHGLPRADPQLF
                     STGTNLVVGLDDGLILKIFPPFLRSQFVSERISLAQLRGRLSVPIPEIVLEGERDQWP
                     YLVITRLPGILGKEAWPALPEDQKERVLAQIGETIAEVQRVPVGDLSRIEPRWRQFMS
                     KQIEGCRARHERLGLPQKYLDGLDELLRDAAALIPLDTPPVMLTGEYIPENFLLSRES
                     GGWRLSGLFDFGDVMTGWGEYDLLGPSAFMTAGMPRRVRSLFEGFGYSNADIDPTLKR
                     RLMALLLLHRFSDPVRHICIEDWQQKAADLFELQELLWPG"
     gene            1548..3164
                     /locus_tag="VN968_18300"
     CDS             1548..3164
                     /locus_tag="VN968_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31222.1"
                     /translation="MKRRDFLKSVSGIAAGAMVPAPAIWSAAKADARSETLLIVSESG
                     PNNLDIHGTGTNVPGYEVSWNCYDRLITHEMKTGPGGVPYYDRDKIRPELAEDMNVGD
                     MSATFKLKKNAKFHDGAPVTAKDVKWSLDRAIGVGGFPAFQMSAGSLTKPEQFVVVDD
                     TTVRVDFAKKDRLTIPDLAVIVPCVVNSELLKKNASEKDPWGLEFAKQQTVGSGAYKV
                     TKWTAGTEVILERNDEWVGGPAPKIRRVIWRMVPQAGNRRALLERGDADVSYDLPNKD
                     FVELKDGGKLNIVSMPYSNGIQYLGMNVTKPPFNNPKVRQAVAYAVPYQKIMDAVLFG
                     LAKPMFGAAGKPTEAVWPQPHTYNTDIAKAKALLAEAGYPDGFETTLSFDLGFAGVNE
                     PLCVLVQESLGQIGIKTTINKIPGANWRTELNKKEMPLYTNVFSGWLDYPEYFFIWCY
                     HGRNSIFNTMGYQSKEMDGFIDGAVAAGANGDKATYDTDVKGFIDRAFTDMPRIPLYQ
                     PYVNVAMQKNVSGYQYWFHRRLDYRAMVKA"
     gene            3381..4394
                     /locus_tag="VN968_18305"
     CDS             3381..4394
                     /locus_tag="VN968_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31223.1"
                     /translation="MLTMIGKRLMFAIPSLIGVVIVTFLLTRALPGDPAAYFAGPAAN
                     KEAIEQIRKKLGLDKPLIEQFFRYTADLAHGDFGNSLTTGQPVATEIRNRLPASAELT
                     LLGLVISVAIALPLGVLAATRPGSWIDHLCRVTTTAGVSLPVFFTGLVLVYIFYFKLG
                     WSPAPLGRLDVFYSAPAAVTGFYLIDALIARDFETFRSAFSQLILPALTLAIFSLAPI
                     ARMTRASMLAVLSSDFVRAARASGLSPGTVIVTYAFRNAMLPVITTLSMVFSFLLGAN
                     VLVEKVFAWPGIGSYAVEALISSDFAPVQGFVLTMAVMYVLLNLMIDILYGVIDPRVR
                     LEG"
     gene            4405..>5246
                     /locus_tag="VN968_18310"
     CDS             4405..>5246
                     /locus_tag="VN968_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31224.1"
                     /translation="MSTVAPAVEPVAPARSSGLAAIFEHTRYVLGENRVTAFAFGLLI
                     VIVFAALFGPYVVPYDPLASDTAAALKPPSSAHWFGTDQLGRDIFSRVIVATRLDTFI
                     AVASVVLVFLMGGLAGISAGYFGGWTDRIVGRIADTIMAFPLFVLAMGIVAALGNTVQ
                     NIIIATAIVNFPLYARVARAEANVRRDAGFVQAARLSGNGEFRILLVHILPNIMPIMI
                     VQMSLTMGYAILNAAGLSFIGLGVRPPTAEWGIMVAEGAGFMVSGEWWIALFPGLALM
                     IAVF"
BASE COUNT          953 a         1594 c         1622 g         1077 t
ORIGIN      
        1 aagccggaga ttcaggccgc ctggcaccag aacaccggct atctgccgat tacccgagcc
       61 gctttcgatt tgacccgcgc gcagggcttc tacgaacgca atcccggtac ggcgatctcg
      121 atcgagcaga tcacattgaa aacgccgacc gaaaattcga ggggagtccg gcttggatcg
      181 ttcgttctga ttcgcgatgt gatcgacgac gaactggagc aggccttcag cggcaagaaa
      241 tccgcgcaag ctgcgctgga ttccgccgtc gagcgcggca accggctgct gcgtcaattc
      301 gagcgggcga atccggatcg atagttgcga catctgatgc actcactgcc gagcctgtcg
      361 aatttcgagg ccttgcaggc atggcgcgcc gacgcgtcgc aatgcctgtc ggcggcgctc
      421 gatatcgcgc gcagtcacgg attgccgcgt gcggatccgc aattgttttc gaccgggacc
      481 aatctcgtcg tcgggctcga tgacgggctg atcctgaaga ttttcccgcc gttcctgcgc
      541 agccagttcg tgtccgagcg catttcgctg gcacagcttc gcggacggct cagcgtcccg
      601 attccggaaa tcgtgctcga aggtgagcgc gaccaatggc catatctggt catcacgcgt
      661 ctgcccggca tcctcggcaa ggaagcgtgg cctgcgctgc ctgaggatca gaaagagcgc
      721 gtcctcgctc agatcggcga gaccatcgcg gaagtacagc gggtgccggt gggcgatttg
      781 tcgcgcattg aaccgcgttg gcgccagttc atgtcaaagc agatcgaagg atgtcgcgcg
      841 cggcatgagc gtctcggact gccgcaaaaa tatctcgacg ggctggacga acttcttcgc
      901 gatgcggcag cgcttattcc cctggacaca ccgcccgtga tgctgaccgg agaatacatt
      961 ccggagaatt tcctgttgag ccgcgaaagc ggaggttggc ggctgtcagg gctgttcgat
     1021 ttcggcgacg tgatgacggg atggggcgaa tacgacctgc tgggcccgag cgccttcatg
     1081 accgccggga tgccgcgccg cgtccgaagc ctgttcgagg ggttcggcta ttcgaacgcg
     1141 gatattgatc cgacactgaa gcggcggctg atggcgctgc ttttgcttca tcgtttcagc
     1201 gatcctgtca ggcacatttg catcgaggat tggcagcaga aggccgctga cttgttcgaa
     1261 ctacaggagt tgctctggcc gggatgatgg caggggctgc gttctcgcat gcgcgccgcc
     1321 tgtgccgcac aagaaaaagg cgtccaaaat atttgcactg tatatgggca aagaatcctc
     1381 tttgtatgca atgagggctc acacttgcgc gacagctgat atggcttcgt tcaaacattc
     1441 aaaagctaaa tcatatgctt agacccatgt tagggttccg ttaagttttg gcacaaacct
     1501 tgcgaagctg ttccaggccg ttccccctcg ggcgtttgga gcatcacatg aagcgtcgcg
     1561 atttcttgaa atccgtctcc gggatcgccg ccggcgccat ggtgcccgcg cctgcgatct
     1621 ggtccgccgc caaggccgac gcccgctccg agaccctgct gatcgtttct gaaagtggtc
     1681 ccaacaatct cgacatccac ggtaccggga ccaatgtgcc cggctacgag gtgtcgtgga
     1741 attgctatga ccggctcatc acccacgaga tgaaaaccgg tcctggcggg gtgccgtatt
     1801 acgaccgcga caagatcagg cccgaactcg ccgaagacat gaatgtcggc gacatgtcgg
     1861 cgaccttcaa attgaagaag aatgcaaaat tccacgacgg cgcgccggtg acggccaagg
     1921 acgtcaagtg gtcgctcgac cgcgcgatcg gcgtcggcgg attcccggcg ttccagatga
     1981 gcgccggctc gctgaccaag ccggagcagt tcgtcgtcgt cgatgacacc accgtgcgag
     2041 tcgattttgc gaagaaagac cgcctcacca ttcccgacct cgccgtgatc gtgccgtgcg
     2101 tggtcaattc cgaacttctc aagaagaatg ccagcgagaa ggatccctgg gggctcgaat
     2161 ttgccaagca gcagaccgtt ggttccggcg cctacaaggt gacaaaatgg accgccggca
     2221 ccgaagtgat cctggagcgc aatgacgagt gggtcggtgg tcccgcgccg aagatcaggc
     2281 gggtgatctg gcgcatggtg ccgcaggccg gcaatcgccg cgcgctactt gaacgcggcg
     2341 atgccgatgt gtcgtatgat ttgcctaaca aggatttcgt cgagctgaag gacggcggca
     2401 agctcaacat cgtctcgatg ccctattcca acggcatcca gtatctcggc atgaacgtca
     2461 ccaagccgcc gttcaataat ccgaaagtgc gccaggcggt ggcctacgcc gtgccttacc
     2521 agaagatcat ggatgccgtg ctgttcgggc tggccaagcc gatgtttggt gccgccggca
     2581 agccgaccga ggcggtctgg ccgcagccgc acacgtataa taccgacatt gcgaaagcca
     2641 aggcgctgct tgccgaagcc ggctaccccg acggcttcga gaccacgctg tcattcgacc
     2701 ttggattcgc cggggtcaac gagccgcttt gcgtgctggt gcaggagagt ctcggccaga
     2761 tcggcatcaa gaccaccatc aacaagattc ccggcgccaa ctggcgcacc gaactgaaca
     2821 agaaggagat gccgctttac accaacgtgt tctcgggctg gctcgactat cctgaatact
     2881 tcttcatctg gtgctatcac ggccgaaatt cgatcttcaa caccatgggc tatcagtcga
     2941 aggaaatgga cggcttcatc gacggcgccg tggccgcggg ggcgaatggc gacaaggcca
     3001 cttacgatac cgatgtgaag ggctttatcg atcgcgcctt caccgacatg ccgcgcatcc
     3061 cgctgtatca gccttacgtc aacgtcgcga tgcagaaaaa cgtttccggc taccagtact
     3121 ggtttcaccg ccgcctggat taccgggcga tggtcaaggc gtgacagcgt gatgggcgtt
     3181 cacaacatga gccgcggcct ctcgttccct ccccccttgc gggggagggt tagggagggg
     3241 ggtaagcaac aagcgccgga ttcgcggcta ccccccaccc caacccttaa gagcgagctt
     3301 cgctcgtctc gaccccgcaa gggagggagc agaaagatcc gcgtcgctgg aaatgatctg
     3361 cgagacgcga ggctcatccc atgctgacca tgatcggcaa gcgattgatg ttcgcgatcc
     3421 cgagcctgat cggggtggtg atcgtcacgt tcctgctgac gcgggcgctg cccggcgatc
     3481 ccgcggcgta tttcgccggc cctgcggcga acaaggaggc gatcgagcag atccgcaaga
     3541 aactcggtct cgacaagccg ctgatcgagc agttcttccg ttacaccgcc gatctcgccc
     3601 acggcgattt cggcaattca ctcaccacag gccagccggt cgcgactgaa atccgcaatc
     3661 gcctgccggc atcggccgag ctgacgctgc tcggcctcgt catttcggtt gccatcgcgc
     3721 tgccgctcgg cgtactcgcc gcaacccgcc caggttcgtg gatcgatcat ctttgtcgcg
     3781 tcaccacgac cgccggggtg tcgctgccgg tattcttcac cggattggtg ttggtctata
     3841 ttttctattt caagctcgga tggtcgccag cgccgctggg gcggctcgat gtgttctaca
     3901 gcgcgccggc ggcggtgacc ggtttctatc tgatcgatgc cctgatcgcg cgcgatttcg
     3961 aaaccttccg ttcggcgttc agccagttga tcctgccggc actgacgctc gcaatcttct
     4021 cgctggcgcc gatcgcgcgg atgacgcgtg cctcgatgct ggcggtgctg tcgtcggatt
     4081 tcgtgcgcgc cgcgcgcgcc agcggcctgt cgcccggcac ggtgatcgtc acctacgcct
     4141 tccgcaacgc gatgctgccg gtcatcacca cgctcagcat ggtgttctca ttcctgctcg
     4201 gcgccaacgt gctggtggaa aaggtattcg cctggcccgg catcggttcc tacgcggtcg
     4261 aagccctgat ctcgtcggac ttcgcgccgg tgcagggctt cgtgctgacc atggccgtga
     4321 tgtatgtgct gctcaatctg atgatcgaca ttctctatgg tgtgatcgat ccaagagtga
     4381 ggctggaagg gtgaggttgg aagcatgagc actgtcgcac ctgccgttga acccgttgcc
     4441 cccgcgcgta gctcgggtct cgccgcgatc ttcgagcaca cccgctatgt gctgggcgag
     4501 aaccgcgtca ccgcattcgc gttcgggctg ttgatcgtga tcgtgttcgc ggcgctgttc
     4561 ggcccttatg tggttccgta cgatccgctg gcgtccgaca ccgcggcggc gctgaagccg
     4621 ccgtcatcgg cgcattggtt cggcaccgac cagttgggcc gcgatatttt cagccgcgtc
     4681 atcgtggcga cgcggctcga caccttcatc gcggtggcct ccgtggtgct ggtgttcttg
     4741 atgggcgggc tcgccggcat ctcggcgggg tattttggcg gctggaccga ccgcatcgtc
     4801 ggccgcatcg ccgataccat catggcgttt ccgctgttcg tgttggcgat ggggattgtg
     4861 gcggcgctcg gcaacaccgt gcagaacatc atcattgcga ccgcgatcgt gaattttccg
     4921 ctctatgcgc gcgtcgcccg cgccgaggcc aatgtgcggc gcgacgccgg cttcgtgcag
     4981 gcggcacgat tgtcgggcaa tggcgagttt cggatcctgc tggtgcacat tctgcccaac
     5041 atcatgccga tcatgatcgt gcagatgtcg ttgaccatgg gctacgccat cctcaatgcg
     5101 gccgggctgt cgttcatcgg gctcggggtg cggccgccga ccgccgaatg gggcatcatg
     5161 gtcgcggaag gcgcgggctt catggtgtcg ggcgaatggt ggatcgcgct gtttcccggc
     5221 ctcgcgctga tgatcgcggt gttctg
//
LOCUS       DATNOI010000459         6167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1889978,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000459 DATNOI010000000
VERSION     DATNOI010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6167
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889978"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            511..1578
                     /locus_tag="VN968_18315"
     CDS             511..1578
                     /locus_tag="VN968_18315"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL31225.1"
                     /translation="MGEQKEAVVVGALGVIGRYIVERLLRQDDWSVIGLSRRAAGAVP
                     RYRHISVDLLDREDAAAKLTGLTEATHVFYAAFQPAAGAAAGYASNIAPNRDMLVNAV
                     TAIDSASSALRRVVLVTGTKYYGSHLGPFKTPARESDPRHMGPNYYFEQIDWLAAFQR
                     GKRWDWVELRPQTLCGFAPGTPMSLAPAIAVYAAISKELGLPLRFPGKPGAYTSIYQV
                     TESVHFANAALWAASTPRCGLEAFNITNGDYFRWKNLWPKLAAAFEMPAGEVQTISLT
                     AHMVDNAPLWRVMTEKYGLKPYAYDELVAWPFADYVFSCDWDVMSDVTKSRRFGFHDV
                     VDSEEMFVRLMREFRAERIVP"
     gene            complement(1941..2942)
                     /locus_tag="VN968_18320"
     CDS             complement(1941..2942)
                     /locus_tag="VN968_18320"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXL31226.1"
                     /translation="MRPIIALAMGDPAGISPELTARLLALDDVVTKAQIVVIGDRRVL
                     DDGARVAAVKLDLISAPPDADLSKEERAPIFIDLGHLDPASVARSTATAEGGRFALAN
                     YRHALTMARDGRAHAVCFTPFNKNAMRLAHPVYDDEISFSADVVGLDGPASEFNVLDG
                     LWNARVTSHISLAEVAAHITRERVLRALRLTDASMRTAGFKRPRIAVAGLNPHAGDGG
                     NSGREEIDAIGPAVEEAGADGIATEGPFPADTVFLRAKSGAFDAVLTMYHDQGQIAMK
                     LMGFDRGVTLLGGFPFPICTPAHGTAYDIAGKGIAGIGAARAAILLAAEMAQKQRRV"
     gene            complement(2989..4497)
                     /locus_tag="VN968_18325"
     CDS             complement(2989..4497)
                     /locus_tag="VN968_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXL31227.1"
                     /translation="MENLEALMHGFGIALSGNHIFLMLIGVLLGILVGVLPGLGAPNG
                     VSLLLPLTFGMQPVSAIILLSSMYWGALFGGSVTSILFNIPGEPSSVATTFDGYPMAR
                     DGRPTTALATAFGSAAFGALAGVVLITLSASWVAQVALAFGPPEYFAVYFLAFASFVG
                     MGGSPPIKTLVSLAIGFALAAVGIDTVSGSVRLTMGIDELVKGISFVVAVMGLFGIGE
                     LLIAVEDEFHVKAISSKVEWREVFRTLAGLPRYSWALLRSAAIGCWMGITPGGPTAAS
                     FMSYGIAKRLSPRHANFGKGEPEGIIAPETADHAAGTSALLPMLSLGIPGSATAAVMM
                     GGLMIWGLNPGPMLFVEQKDFVWGLIASMYLGNVVAVVLVLLTVPIFAALMRVPFFII
                     APVIVIICTVGAYSVSNSYLDVVLMMGFGILGYLFKKLHYPLAPLVLAIVIGDKAEDA
                     FRQSMLMSRGSLGIFFAKPLVTTLILLGAALLLMPVIWRTIGRVMPRSQLRP"
     gene            complement(4510..4977)
                     /locus_tag="VN968_18330"
     CDS             complement(4510..4977)
                     /locus_tag="VN968_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXL31228.1"
                     /translation="MVSGRGLEAATALITGAFGAAVVVSSLDNGIGWSAAGVESGTFP
                     FIVGLVILAGSVFNLVQGWLHARAVILGPSELRRLGILFVPAAVFVGIIPLIGMYPAS
                     AFYVFGALAWHKRGSLLLPALAAIGSALALYLIFELTFQISLPRGALGILMGF"
     gene            complement(4988..5992)
                     /locus_tag="VN968_18335"
     CDS             complement(4988..5992)
                     /locus_tag="VN968_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL31229.1"
                     /translation="MSQKPKFAAIAFGLAIGLAGAANAAWQPAKPIEFIATAGPGGGT
                     DNFARAIQNIITIHKLVEQPIVVTNKGGGSGAEGYTYTKAMAGDPYKIVFGTSNAWTQ
                     PMVSKVAYNYTDLTPIAAMVQDEFLLWVKQDAPYQNVQDFLKAVAAKPPGDFKMGGAQ
                     SKDTDETLTRMIDKAAKVKFIYIPFKSGAEAAVQLAGGHIDSHVNNPSESIGQWKGST
                     QRPLCAFSPQRLPDGPKVTATQNWHDIPTCVESGLDIPQFQQPRTVWLPGKVTADQAA
                     FYVDLMKKVQATPEWKEYIERTSQTDTFLTGEAFGKFIKDDIARTHKIAADEGWLVSN
                     "
BASE COUNT         1251 a         1903 c         1832 g         1181 t
ORIGIN      
        1 caacattcac gccaaaacgc gaaagttgtg gaagcattct gccatgcggc ttgccgcctt
       61 tcgaaccaat gccgcccatc aattcaccaa ccgcccgttg atgaattccg tatgcccgcc
      121 gagatgtcca ttgcactctc cgggctcacc tgcatgcgcg catgcacgtc gcctcgtgct
      181 ggcgccgcgt ccaccatcga gatcgccacc aacatacgat ccccatagca ctcgctgcac
      241 tgccgggtgc catacttccc gcgacttcgt gccttggcgc ttttcggacg ccggccgccg
      301 cagcgcgtgg atagcctctt catgccggcg tccgaaaacc tacacagact cggacatcag
      361 ccgctcactc gatcatctca tcggcgagcg cgaggaggtt gtccgggata ttcacgccga
      421 gcgccccggt gtccgccggg cagcgtccca atgcaggcga caagagcctt ggacacggct
      481 accatcgtag tcgaaaggaa tccggcacac atgggtgagc aaaaagaggc cgtagtcgtc
      541 ggagcactcg gagtgatcgg acgctacatc gtcgagcggc tgctcaggca ggacgactgg
      601 tccgtaatcg gtctgtcgcg ccgtgcggcc ggcgctgtgc cccgctaccg ccacatcagt
      661 gtcgatctac tagaccgtga ggacgcggcg gcgaagctca ccggcctgac ggaagccacg
      721 cacgtcttct acgcggcgtt tcagcctgcg gcgggcgccg ccgcgggcta tgcatcgaac
      781 atcgctccaa accgcgacat gctggttaac gcagtgaccg cgatcgacag cgcgtcgtcc
      841 gcgttgcgcc gcgtcgtgct ggtcaccggc acaaaatact atggctcaca cctcgggcca
      901 ttcaaaacac cggcacgaga gagtgacccg cgccacatgg gtcccaacta ttatttcgag
      961 cagatcgact ggctcgccgc cttccagcgc ggcaagcgct gggactgggt ggagctgcgg
     1021 ccgcagacct tatgcggctt tgcgccgggc acgccgatga gtctggcgcc ggcgatcgcg
     1081 gtctacgccg caatcagcaa ggagcttggg ttgccgctgc gtttcccggg aaagcctggc
     1141 gcctatacat cgatctacca ggttaccgaa tccgtgcact tcgccaacgc ggcgctctgg
     1201 gcggcgagca cgcctcgctg cgggctcgaa gctttcaaca tcaccaacgg tgactatttc
     1261 cgctggaaga atctgtggcc gaagctcgcg gccgctttcg aaatgccagc cggcgaggtg
     1321 cagacgataa gccttaccgc acacatggta gacaacgcac cactctggcg cgtcatgaca
     1381 gagaaatatg ggctgaagcc ctacgcttac gatgagctcg tggcctggcc ctttgccgat
     1441 tacgtcttca gttgcgactg ggatgtgatg tcggacgtta ctaaatcgcg ccgcttcggc
     1501 ttccacgatg tggtcgatag cgaagagatg ttcgtgcggc tcatgcgcga gttccgcgca
     1561 gagcggatcg ttccctgaga cccatcgccg ccgcgacaac gatcgtctgc atgacgtcgg
     1621 ctcagggtca aaagctgact tccagagaat gtcggctttt ggcggatagt gttgaaaaag
     1681 tcttttttgg gtgacgagcg aaattttcca gaaccgctga tgcgtttcgt acgtggcgac
     1741 gtgagggacc acattgtttc gcacaaaaac gaccacggac catcgtatcg gcgctacagg
     1801 gcgctgcagc agtagagaca tctaaaaatc tactttcgcg agattttcgg tatcgttcga
     1861 ttttcgactt ttgcaacagt atcggcgcaa agccgacatt cgccaggcga cccgcctcag
     1921 cgaggtgcgg gctggcggct tcaaacccgc cgttgtttct gcgccatttc cgcggcaagc
     1981 aggatagccg cgcgtgcggc tccgattcct gcgattccct ttccggcgat gtcgtaggcc
     2041 gttccgtgcg ccggcgtaca tataggaaaa ggaaagccac caagcagcgt aacgccgcga
     2101 tcgaagccca tcagtttcat cgcgatctgg ccttggtcat gatacatggt cagcacggcg
     2161 tcgaatgcgc cgctttttgc gcgcaggaac acggtatctg cgggaaacgg gccttctgtc
     2221 gcgatcccgt cagcgccggc ttcttccacc gcgggtccaa tcgcgtcgat ctcttcccgc
     2281 ccggaatttc cgccatcgcc ggcatggggg ttgaggccgg cgaccgcgat gcgcggacgc
     2341 ttgaatccgg ccgttcgcat cgaggcatcc gtgagcctga gggcgcgcag gacgcgttcc
     2401 ctggtgatat gcgctgcgac ctccgcaagc gagatatgcg atgtgacgcg cgcgttccaa
     2461 agcccgtcca gtacgttgaa ttcgctcgcc gggccgtcaa gcccgaccac gtcggccgaa
     2521 aagctgatct cgtcatcata gacgggatgt gcaaggcgca ttgcattctt gttgaatggc
     2581 gtaaagcaga cggcgtgggc ccggccgtca cgtgccattg tcagcgcgtg acgataattt
     2641 gccagcgcaa accggccgcc ttcggccgtc gcggtgcttc gcgcgaccga tgccggatcg
     2701 agatggccga gatcgatgaa aatcggtgcc cgctcttctt tcgacaggtc ggcgtccggt
     2761 ggggcagata tcaagtcaag cttcaccgcg gcaactcgcg ctccgtcgtc gaggacgcgg
     2821 cgatcgccga tgacgacgat ctgcgctttg gtgacgacgt catcgagggc gaggagtctg
     2881 gcagtcaatt ccggactgat gccggcagga tcacccatgg cgagcgctat aatcggtctc
     2941 atcaggatct cccgggcctt tctgcggtca atttgttctg ctcgatcatc atggccttaa
     3001 ctgcgaccgc ggcatgactc tgccgatcgt tcgccagatc actggcatca gcagcagcgc
     3061 cgcgccgagc aagatgagcg ttgtcaccag cggtttcgca aaaaatattc ccagcgaacc
     3121 tctcgacatc agcatggact ggcgaaatgc atcttccgcc ttgtcgccaa tcacgatcgc
     3181 gagcaccagc ggcgccaggg gataatgaag tttcttgaac agatatccca aaatgccgaa
     3241 tcccatcatc aacacgacat cgagatagga attagaaacc gaataggctc cgaccgtgca
     3301 gatgatgacg atcactggcg cgatgatgaa gaacggcacg cgcatcagcg ccgcgaagat
     3361 cggcaccgtc aatagcacca gaaccacggc cacgacgttg ccgagataca ttgaagcgat
     3421 caatccccag acgaagtcct tttgctcgac gaacagcatg ggtccgggat tcagtcccca
     3481 gatcatcagg ccgcccatca tgacggctgc ggtagccgat ccggggatgc ccaacgacag
     3541 catgggaagc agggcgctgg tgccggcggc atgatccgcg gtttccggag cgatgatgcc
     3601 ctccggctcg cctttgccaa aattcgcgtg ccggggtgaa agacgcttgg cgatcccgta
     3661 gctcatgaac gatgccgcgg tcggcccccc cggtgtgata cccatccagc atccgatcgc
     3721 cgcgctgcgc aacagcgccc aactgtatcg cggcaggccc gcgagcgtac ggaagacttc
     3781 gcgccactcg actttggaag agatcgcctt gacgtgaaac tcgtcttcca cggcaatcag
     3841 caactcgccg ataccgaaca ggcccatgac ggcgaccacg aagctgatac ccttcaccaa
     3901 ctcgtcgatt cccatggtga gccgtacgct gccggaaacc gtgtcgatcc cgacggccgc
     3961 gagcgcaaag ccgatcgcga gcgacaccag cgtcttgatc ggcggcgagc cgcccatgcc
     4021 cacgaagctc gcaaacgcga gaaaatagac cgcgaaatat tcgggcggcc caaaggccag
     4081 cgccacttgc gcgacccatg acgccgagag cgtaatgaga acaacaccgg cgagcgcgcc
     4141 aaaagcggcc gagccaaaag cagttgccag cgccgtggtc ggcctgccgt cgcgcgccat
     4201 tggatagccg tcaaacgtgg ttgcgaccga ggagggctcg cctggaatgt tgaagaggat
     4261 ggaagtgacc gagccgccga acaatgcgcc ccaatacatc gatgagagca ggatgatggc
     4321 cgataccggc tgcatcccga aggtgagcgg cagcagcagc gatacgccat tgggcgcccc
     4381 gaggccgggc aacacgccga cgagaatgcc gagcagcaca ccgatcaaca tcaggaaaat
     4441 atgattgccg gacagcgcga tgccaaaacc atgcatgaga gcttcgagat tttccaacga
     4501 agcgctccct tagaaaccca tgaggatgcc gagcgcgccg cgcggcaacg agatctggaa
     4561 cgtcagctcg aatatcagat agagcgccaa cgccgagccg atcgcggcga gggccgggag
     4621 gagaagcgag cctcgcttgt gccaggccag cgcgccgaac acatagaagg cagaggccgg
     4681 atacatcccg atcagcggaa tgattccgac aaaaaccgcg gccgggacga acaatattcc
     4741 caaccgcctg agttcgcttg gcccgaggat gacggcccgg gcgtgcagcc atccctgcac
     4801 gagattgaac acactgccgg ccagaatgac gaggccgacg atgaacggaa aggtgccaga
     4861 ttcgacgccg gcggcggacc agccgatacc gttgtccagg ctggagacca cgacggctgc
     4921 gccgaacgcc ccggtgatga gcgccgtcgc cgcttccaga ccgcgtccag agaccatcat
     4981 ccttgctcta gttggagacc agccagccct catcagcggc aatcttgtgg gtgcgcgcga
     5041 tgtcgtcctt gatgaatttg ccgaacgcct cgccggtcag gaaggtgtcg gtttgcgacg
     5101 tccgctcgat atattccttc cattccggcg tggcctgcac cttcttcatc agatcgacat
     5161 agaaggcggc ctgatccgcg gtcacctttc ccggcagcca gaccgtgcgg ggttgctgga
     5221 attgcggaat gtcgaggccg gattcaacgc aggtcgggat gtcgtgccag ttttgcgttg
     5281 ccgtgacctt ggggccgtca ggaagacgct gcgggctgaa ggcgcacagc gggcgctggg
     5341 tgctgccctt ccactgtcca atgctttcgc tgggattgtt gacgtgggag tcgatatggc
     5401 cgccggccag ttgcaccgca gcctcggcgc cgctcttgaa gggaatgtag atgaatttta
     5461 ccttcgccgc cttgtcgatc atgcgggtga gggtttcatc ggtgtccttt gactgcgcgc
     5521 cgcccatctt gaaatcgccc ggcggcttcg cagcaacggc tttgagaaaa tcctgcacgt
     5581 tctggtatgg cgcatcctgc tttacccaga gcagaaactc gtcctgcacc attgcggcaa
     5641 tcggcgtcag gtcggtgtag ttataggcga ccttggagac catcggttgg gtccaggcat
     5701 tcgatgtgcc gaacacgatc ttatagggat cgcccgccat cgccttggtg taagtatagc
     5761 cttcggcgcc gctacccccg cccttgttgg tgacgacgat cggctgctcg accagtttgt
     5821 gtatggtgat gatgttctgg atggcacgcg cgaaattatc cgtgccgccg cccgggcccg
     5881 ctgttgcaat gaattcgatc ggtttggctg gttgccacgc ggcatttgcc gctcccgcta
     5941 ggccaattgc gagaccgaag gcgatcgcag cgaatttcgg cttctgagac atttagtcct
     6001 cccatgtgtc ttttgttgat ttttgtcttc ttgttcttgg ctttttgcgt tagcccactt
     6061 catcctcagc aaagtctgct gcggcaatag ctccttcttc gtgaagcgcg gcaatttcgt
     6121 cggcgtcaaa accgtaagcg cccaggattg cactggtgtg ctcgccg
//
LOCUS       DATNOI010000460         2111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_411165,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000460 DATNOI010000000
VERSION     DATNOI010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411165"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /gene="kdpA"
                     /locus_tag="VN968_18340"
     CDS             <1..1006
                     /gene="kdpA"
                     /locus_tag="VN968_18340"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HXL31230.1"
                     /translation="TNGGGFFNANAAHPFENPTALSNFVQMISIFSLGAALTNVFGRM
                     VGNQRQGWAILAVMGVLFIAGVTVTYWAETNGTTALDALGLTGGNMEGKEVRFGISAS
                     SLFAVITTAASCGAVNAMHDSFTALGGMIPLINIQLGEIVVGGVGAGLYGMLLFVVLS
                     IFVAGLMVGRTPEYVGKKIEAREVKMAMLAILVLPLMYLGWTAVAVVLPSAVASMANT
                     GPHGFTEVLYAYTSQTGNNGSAFAGLTGNTPFYNITGGVAMFVGRFFMIIPAMAIAGS
                     LVQKKSIPPSAGTFPTTGGLFVGLVVGVILIIGGLTFFPALALGPIVEHLAMNANSLF
                     "
     gene            1032..>2111
                     /locus_tag="VN968_18345"
     CDS             1032..>2111
                     /locus_tag="VN968_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HXL31231.1"
                     /translation="MDVVKLNKRMPVSAMLDPKIVMPAIGAAVTKLDPRLMIKNPVMF
                     VVETVAALTTVIFLRDLVTGGEHLGFTFQIIFWLWFTVLFANFAEAVAEGRGKAQAES
                     LRKTRTESQAKLLSGSDTTYRLVPGTSLKVGDVVLVEAGDNIPSDGEVIEGVASVNEA
                     AITGESAPVIRESGGDRSAVTGGTQVLSDWIRVRITAEQGSTFIDRMIRLVEGAERQK
                     TPNEIALNILLAGLTIIFVFATVTIPSYASYAGGSISLVVLVALFVTLIPTTIGALLS
                     AIGIAGMDRLVRFNVLAMSGRAVEAAGDVDTLLLDKTGTITLGNRQATEFRPLRGVAE
                     QELGDAAQLASLADETPEGRSIVVLA"
BASE COUNT          338 a          699 c          659 g          415 t
ORIGIN      
        1 caccaacggc ggcggtttct tcaacgccaa cgcggcgcat ccgttcgaga acccgacggc
       61 gctctcgaac ttcgtgcaga tgatctcgat cttctcgctt ggcgctgcgc tcaccaacgt
      121 gttcggccgc atggtgggca accagcgcca aggctgggcg atcctcgccg tcatgggtgt
      181 actgttcatt gccggcgtca cggtcaccta ttgggcggaa accaatggca ccaccgcgct
      241 cgacgcgctg gggctaaccg gcggcaacat ggaaggcaag gaggtccgct tcggcatctc
      301 ggcctcgtcg ctgtttgccg tcatcacgac ggccgcctcc tgcggcgcag tcaacgccat
      361 gcatgacagc ttcaccgcgc ttggcggcat gatcccgctg atcaacatcc agctcggcga
      421 aatcgtcgtc ggcggtgtcg gcgcgggcct ctacggcatg ctgctgttcg tcgtgctgtc
      481 gatcttcgtc gcagggctca tggtcggccg cacgccggaa tatgtcggca agaagatcga
      541 ggcgcgcgag gtcaagatgg cgatgctcgc gatcctggtc ctgccgttga tgtatctcgg
      601 ctggaccgct gtcgccgtcg tgctgccttc ggcggtggcc tcgatggcca ataccggacc
      661 acatggcttc accgaggtgc tctatgccta tacgtcgcaa accggcaata acggctcggc
      721 cttcgcaggg ctgaccggca acacgccctt ctacaacatc accggcgggg tcgcgatgtt
      781 cgtcggccgc ttcttcatga tcattccggc gatggcgatc gcgggttcgc tcgtgcagaa
      841 aaagtcgatt ccgccatcgg ccggtacctt cccgaccacc ggcggcctgt tcgtcggcct
      901 cgtcgtcggc gtgatcctga tcatcggcgg attgaccttc ttcccagcgc tggcgcttgg
      961 gccgatcgtc gagcatctcg cgatgaacgc caactcgctg ttctgatcga tctcatttcg
     1021 gagttgctcc catggacgtc gtcaaattga acaagcgcat gccggtatcg gcgatgcttg
     1081 acccaaagat cgtgatgccc gcgatcggcg cggccgtcac caagcttgat ccgcggctca
     1141 tgatcaagaa ccccgtgatg ttcgtggtcg agaccgtggc cgcactcacg accgtgatct
     1201 tcctgcgtga tctcgtcacc ggcggggaac atctgggctt caccttccag atcatcttct
     1261 ggctgtggtt cacggtgctg ttcgccaact tcgccgaagc cgttgccgaa ggccgcggca
     1321 aggcgcaggc cgagtcgtta cgcaagaccc gcaccgagag ccaggccaag ctgttgtccg
     1381 gttctgacac gacttaccgg ctcgtgcctg gcaccagcct caaggtcggc gacgtcgtgc
     1441 tggtcgaggc cggcgacaac atcccctccg acggcgaagt catcgagggc gtggcgtcgg
     1501 tgaacgaggc ggcgattacg ggcgaatccg cgccggtgat ccgcgaatcc ggcggcgacc
     1561 gctcggccgt gaccggcggc acgcaggtgt tgtccgactg gatccgggtc cgcatcaccg
     1621 cggagcaggg ttcgaccttc atcgaccgca tgatccggct ggtcgagggc gccgagcggc
     1681 agaagacgcc gaatgagatc gccctcaaca tcctgctcgc cgggctcacc atcatcttcg
     1741 tgttcgcgac cgtgaccatc ccgagctacg cctcctatgc cggcggttcg atttcgttgg
     1801 tggtgctggt ggcgctgttc gtgaccttga tcccgaccac catcggcgcg ctgttgtcgg
     1861 ccatcggcat tgccggcatg gaccgcctgg tgcgcttcaa cgtgctggcg atgtcgggcc
     1921 gtgcggtcga ggccgccggc gacgtcgata cgctgctcct cgacaagact ggcaccatca
     1981 cgctcggcaa ccgccaagcc accgagttca ggccgctgcg cggcgtcgcc gagcaggaac
     2041 taggcgatgc cgctcagctc gcctcgttgg cggacgagac cccggagggt cgatccatcg
     2101 tcgttctcgc c
//
LOCUS       DATNOI010000461         8687 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_315826,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000461 DATNOI010000000
VERSION     DATNOI010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315826"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VN968_18350"
     CDS             complement(<1..122)
                     /locus_tag="VN968_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HXL31232.1"
                     /translation="MNKELAHMLRTAPLSTPTALSADAVRDISAALTALLADVFA"
     gene            complement(333..3242)
                     /locus_tag="VN968_18355"
     CDS             complement(333..3242)
                     /locus_tag="VN968_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXL31233.1"
                     /translation="MSGVPGKRTQRENGIDAGATTDEVISFGPFRLHAAQRLIERKGT
                     PLHLSGRALDILIALVEQASRVVSKNELMARVWPDATVDESSLRVHVAALRKALGDGE
                     AGARYVTTLTGQGYCFVAPVSRSSEPALPMTANSKSGQAHNLPTRPTRMVGRDQTVQE
                     ISEQLKTERFVTIAGPGGIGKTTVAVSVGHELLAEFAGAAHFFDLGPLNDPVLVPGAV
                     ASTLGLLVQSADPIPGLIAFLRDKRMLLILDSCEHVIETAAALAERIFEGAPQAHILA
                     TSRESLRVEGEHVHRLSPLASPPDDAGLTAAQALDFPAVQLFVERAIASDRRFELNDA
                     DAPVVGEICRRLDGMALAIELAAGRIDVYGVQETMALLNDRFKLLWDGRRTALPRHQT
                     LSATLDWSYDLLSTLERSILCRLSVFVGVVTLEGARAVATGDDIDETQVVTVLVGLVA
                     KSLISADTSVPATRYRLLDTTRAYASAKLSESGEAGDVKRRHAKYFLELFERVDGNSF
                     TPLQAKSFAIFADQLGNVRTALEWCFSERGNGVLGVALAAASAPLFLEMSLLTECHRW
                     TERAVAALDDGNRGTRREMDLHAALGLSLMFTRGNSEQVGNSLLRGLELAERLGDLHS
                     QLRLLERLHLFHVRIGNFREALAFAQRGEGVATEIGDPVLLGQIQVALGISHHLEGNS
                     IAALGYLESALAQLPLSPQINSLHLGFDYRNRARITLARVLWIRGCPDQAVTFARQSV
                     EEAIHLNHPIKLCMALLWAMSVFLWNEDLESAEEYTDRFTAQAEKHALAPYQTIGLGA
                     KGELLVRRGDVEAGIVLLRRSLEALRAHRYGLQTAFSTALAEGLSMTGRGDEALDTID
                     EAIALVEHNEDLFGMPELLRIKADILVSAQPPDPVRAEGLLIQSLEMARQQSALAWEL
                     RTATSLARLWVTQDRFGEARDVLAPAYDRFTEGFDSPGLKTARQLLDELRIRTGA"
     gene            complement(3488..3880)
                     /locus_tag="VN968_18360"
     CDS             complement(3488..3880)
                     /locus_tag="VN968_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31234.1"
                     /translation="MAEGLAKTGQRDAALIAVDDAITRVESNGQAVGLAELLRIKATI
                     LMSAPQADLLRAEELLQGSLELAGRQHALAWELRTATSFARLRSMQGRHQEARDLLAP
                     VFDQYTEGFESSDLKAAKALLDRLAIIL"
     gene            complement(3972..5399)
                     /locus_tag="VN968_18365"
     CDS             complement(3972..5399)
                     /locus_tag="VN968_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31235.1"
                     /translation="MASKPGQAHNLPTRLTRMVGRDQTVQEISEQLKTERFVTIVGPG
                     GIGKTTVAVSVGYELLTEFAGAIQFLDLGPLNDPLLIPGTVASALGLPVQSADPTRGL
                     IGFLRGKRMLLILDSCEHVIETAAALAERIFEEAPQVHILATSRESLRVEGEHVHRLS
                     PLGSPPDDAGLTAAQALDFPAVQLFVERAIASGRRFELNDADAPVVAEICRRLDGIAL
                     AIELAAGRADAYGIQETLVLLNDRFKLLWEGRRSALPRHQMLRATLDWSYDLLSEHER
                     LVLRRLSVFTGIFTLDAARSVAADDDVDGAQILTSVVSLVTKSLIAVSAGDTTTRYRL
                     LDTTRAYAAGKLVESGEADAIKRRHAIYYRELFERTETDLLVSTNDSNLRSELLGNAR
                     VALEWSFSSRDDVGIGTSLAAVAAPFLSELSLLTECHRLTGRALAALGDADRGTRQEM
                     ELQASLGSSLRGRPWPEGRTGDPLR"
     gene            complement(5474..5815)
                     /locus_tag="VN968_18370"
     CDS             complement(5474..5815)
                     /locus_tag="VN968_18370"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXL31236.1"
                     /translation="MVGTGLSRLALQYYSEMSQGIKENPQTDEIISFGPFRLYAAQRL
                     IEREGVPLHLGGRALDIFIVLVEHAGEVVSKNDLMARVWPGVTVDEGSLRVHVAALRK
                     ALGDGESEPGI"
     gene            complement(5901..7250)
                     /locus_tag="VN968_18375"
     CDS             complement(5901..7250)
                     /locus_tag="VN968_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXL31237.1"
                     /translation="MSPAARRILLSVVAIVVVAVAIGLWIVRGPGPMAFADGPKVALA
                     DYRGANPAGVPAALAQASVVERGAYLARAADCMVCHTTAGGKEYAGGLGFKLPFGTLY
                     STNITPDKETGIGNYSDQDFLAAMHRGIRRDGARLYPAMPFTSYTYISDADALAIKAY
                     LFSLPPVRAAASENTLTFPFNQRWAMGFWSALFNPDTRFQPDTSKSPEWNRGAYLAEA
                     LAHCGECHTPRNLAFALNNRLKFAGAVTAGWRAFNISSDKTTGVGAWRDDDLVSYLSI
                     GHADGHGTASGPMGEAVDHSLSKLAPEDIRAVVAYLRSVPPTASTDLPATLAPSAPAS
                     HRDGGGAPDPRGKMVFEGACVSCHGWSGESSISPFATLTGAWAVNDPGATNVAQIVIS
                     GTKRHTPEGAVSMPAFGNAYSDVEIAAVANYVTARFGSKPAHMTAQDVAALRKQTSQ"
     gene            complement(7247..>8687)
                     /locus_tag="VN968_18380"
     CDS             complement(7247..>8687)
                     /locus_tag="VN968_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL31238.1"
                     /translation="KVDDSAARKIPGVQQIVVLDDMVAVVGDHMWAAKKGLDALKVTW
                     NEGPNARISSKDIWQDLRTASEKDGAVAKSIGDIAKGLATGEKLEAAFELPFLAHATM
                     EPLNATVHFKPDSCEIWTGTQIVSRVQAEAAKAAGLAVEKVIVNNHLLGGGFGRKLEP
                     DMVVAAVRVAKQVDGPVKVVWTREEDIQHDVYRPVYRDTIAATLSDGKIVGWKYRVTG
                     SAILARWLPPAFQKGIDIDAIDAGADMPYDIPNFHVEYVRAEPPAVPTGFWRGVGPNN
                     NVFAVECFMDELARKAGKDPVEFRRSMLGKKPRLLAALNLAAEKSNWGQPLPARVGRG
                     VCVQPSFETFIATVVEAEVDEHGEVQLRRVTSVVDTGIAVNPDTIKAQLEGGLIFGLT
                     AALYGEITIDKGRVQQSNFHDYRMLRIDQTPKIEVHVVKSGEPPGGIGETGVNAGPPA
                     LRNAIYAATGVALRRLPIDRALISAGKKA"
BASE COUNT         1682 a         2820 c         2606 g         1579 t
ORIGIN      
        1 gcgaacacgt ccgcaaggag cgcggtcagc gcagctgaaa tatcgcgaac ggcgtccgcg
       61 gaaagcgcgg taggcgtcga aagcggagcg gtgcgcagca tgtgcgccaa ttccttgttc
      121 atcgctgaat tccttatcga gggacggccg gatgccaaag catggtatcc ggttatctgg
      181 attcatcgcc gcaccatgca aggaatggac gcccgcatct tccgggaaca gagacatttt
      241 ttcttgaacg cacggctttc agccatcaag cattcgtgtg caaagacgcg agcaaacatg
      301 cctgcgacac caatacgcgt cgatgtcctc gatcaggcgc cggttcgtat tcgcaactcg
      361 tccagcaact gcctggccgt cttcaggccg ggactatcga agccttcggt gaaccggtca
      421 tatgccggcg ccagcacgtc ccgcgcctcg ccgaaacggt cctgcgtcac ccacaaccgc
      481 gccagacttg tcgcggttcg caattcccat gcgagtgccg attgctgcct cgccatctcg
      541 agcgactgaa tcaggagacc ctcagcccgg actggatccg gcggttgcgc cgaaaccaga
      601 atatccgcct tgatacgcag cagttcgggc atgccgaaca aatcctcatt gtgctcaacc
      661 aatgcgatgg cttcatcgat ggtatcgaga gcttcgtctc cccgtcccgt catcgagaga
      721 ccttccgcca gcgcagtgct gaaagccgtc tgcaagccgt atcgatgagc gcgtaatgct
      781 tccaaagaac ggcgcagcag gacgatgcca gcttccacgt caccgcgcct gaccaggagc
      841 tcgcccttgg cgcccaggcc gatcgtctgg tatggcgcca gcgcgtgttt ttcggcctgc
      901 gcggtgaatc tgtcggtgta ttcctcggcg ctttccagat cctcgttcca cagaaaaacc
      961 gacatcgccc agagcagcgc catgcacaat ttgatcggat gattgaggtg gatggcttcc
     1021 tcaacggact gtcgcgcgaa cgtcaccgcc tgatccgggc agccccgaat ccaaaggact
     1081 cgggccaggg taatgcgagc gcgattgcga tagtcgaagc cgagatgcag agagttgatc
     1141 tgtggcgaaa gcggcagctg cgccaaagcg gattccagat agccgagagc ggcgatgcta
     1201 ttgccttcca gatggtggga aatcccaagc gccacctgaa tctgcccgag cagaaccggg
     1261 tcgccgatct ctgtcgcaac accttcgcca cgttgggcaa atgccagcgc ttcccggaaa
     1321 ttcccaattc tgacatggaa gaggtgaagt cgttcaagca gacgaagctg actatgcaag
     1381 tcgcccaatc gctccgcaag ctcgagacct cggagcagcg agttgccgac ctgctcgctg
     1441 ttgcctctgg taaacatgag cgacaggcca agggccgcgt gaaggtccat ctcacgtcga
     1501 gtacctcgat tgccgtcatc gagcgccgcg accgcccgct ccgtccagcg atgacattcg
     1561 gtcaatagcg acatctcaag aaacagcggg gccgaggccg cagcgagagc aacgcccagc
     1621 acgccattgc cgcgttccga gaaacaccat tccagcgcgg tgcggacatt tccgagctga
     1681 tctgcaaata tcgcaaaact cttcgcctgc aggggcgtaa acgaattgcc gtcgacccgc
     1741 tcaaataact caaggaagta ctttgcgtga cgtcgcttca cgtcgcccgc ttcaccgctt
     1801 tcggacagct ttgcggaagc gtaggcccgc gtggtgtcaa gcaggcgata tcgcgtcgcc
     1861 ggcacactgg tgtccgcgga tatcagggat ttcgcaacca gaccgaccag gaccgtgacg
     1921 acctgagttt catcgatgtc atcgcccgtc gcgaccgcgc gagccccttc gagagtgacg
     1981 acgccgacga acaccgaaag cctgcacagg atcgaacgct caagcgtcga caacagatca
     2041 taactccagt ccagcgtcgc gctcagcgtc tgatgccgcg gcagcgccgt gcgccggcca
     2101 tcccagagca gcttgaagcg gtcgttcagc aacgccatgg tttcctggac gccgtaaaca
     2161 tcgattcggc cggctgcgag ttcgatcgca agggccatcc cgtccagccg ccggcagatt
     2221 tcgccaacga caggcgcatc ggcgtcgttc aactcaaagc ggcggtcgct cgcgatcgcg
     2281 cgctcgacga acagctgcac tgccggaaaa tcgagcgcct gtgccgccgt gagaccggca
     2341 tcatcgggcg ggctagccag cggcgacagc cgatgaacgt gctcgccttc gacacgcagc
     2401 gattcccggc tggtcgccag aatatgcgcc tgcggcgcgc cctcgaaaat ccgctccgcc
     2461 agcgccgccg ccgtctcgat cacatgctcg cagctatcga ggatcaatag catccgcttg
     2521 tctcggagga aggcgatcag accggggatt ggatctgccg actgaaccag gagtccaagt
     2581 gtcgaggcga ccgcgcctgg caccagaaca ggatcgttga gcgggccgag atcgaaaaag
     2641 tgagcggcgc cggcaaactc cgccagcagt tcatggccca cagagacggc gacagtggtc
     2701 ttgccgatgc cacccggtcc ggcgatggtg acgaaacgct cggtcttcag ttgctccgat
     2761 atttcctgaa cggtttggtc acgccccacc attcgtgtcg gtcgggtcgg cagattgtga
     2821 gcctggccag acttcgagtt cgccgtcatc ggaagcgccg gctcgctcga acgcgagacc
     2881 ggcgccacaa agcaataacc ctgtccggtg agcgtggtga catatctggc gcccgcctcg
     2941 ccgtcgccca gcgccttgcg aagtgcggcg acatggacgc gaaggctact ctcgtcgacc
     3001 gtggcgtccg gccagacccg cgccatcaat tcattcttgc tgacaaccct gctcgcttgc
     3061 tcgaccagag cgatcaggat atcgagcgcc cggccgctaa gatgaagcgg ggtacccttc
     3121 ctttcgatca gtcgctgcgc cgcgtgcagc cggaacggac caaatgagat gacttcgtcc
     3181 gtcgtcgcgc cggcatcgat tccattttcg cgctgagtgc gtttaccggg cacgcccgac
     3241 attgtttggg ccctcgaaga taagtccaac tctgtgatcg accacgccga caggcgatat
     3301 cggacgctca atttaagcgt gtagatcggc gatctcaagt ttacccgagg cgaccggcgg
     3361 cgacgatctc cttggaggcg acggattgtc gcccggtttg cccataatgt gttcaacagg
     3421 ataccaggga gacgatgggg tcccgagcaa gccaacggat ttgacctgcg acacggcctg
     3481 cccttggtca caaaatgatc gccagcctgt ccagcaacgc cttcgctgct ttcagatcgg
     3541 aactctcgaa gccttccgtg tactgatcaa ataccggcgc cagcaagtcc cgcgcctcct
     3601 gatgacggcc ctgcatcgac cgcaaccgcg cgaaactcgt cgcggttcgc agctcccagg
     3661 ccagcgcgtg ctgcctgccg gccaattcga gggatccctg caaaagctct tccgcccgga
     3721 gcagatccgc ctgtggcgcg gacatcagga tcgtcgcctt gatacgcagc agctccgcca
     3781 agccgacagc ctgcccgttg gactcgaccc gggtaatcgc gtcatcgacg gcaatcaagg
     3841 ccgcatctcg ctggccggtc ttcgcgaggc cttccgccaa aatgccgaga aatgtcggat
     3901 tgtgcagctc gtagcgatca gcccgcaacg tatcaatggc gtgacgcaac aactgaacgc
     3961 cagcttcggc ttcaccgtaa tggatcgcca gtgcgccctt caggccaagg ccgacccctt
     4021 agcgatgatc ccagcgaagc ctgaagctcc atttcttgcc gcgtgccacg gtcagcgtcg
     4081 ccgagtgccg cgagcgccct gccggtcagg cgatggcatt cggtcagcaa cgacagctcg
     4141 gataagaatg gcgcggcgac ggctgcaagc gatgtgccga tgccgacatc gtcccgcgac
     4201 gagaagctcc attccagtgc gacgcgggcg ttgccgagca gctccgagcg caaattggaa
     4261 tcgttggtcg ataccaaaag atcggtttcg gttcgctcga acagttcacg atagtaaatc
     4321 gcgtggcgtc gcttgatcgc gtccgcttcg ccgctttcga ccagcttgcc cgcggcatag
     4381 gcgcgggtgg tgtcgagcag ccgatagcgc gtggtcgtat cgcctgcact caccgcgatg
     4441 agagatttcg taaccagact tacaacggac gttaggattt gtgcaccgtc aacgtcatcg
     4501 tctgcggcga ccgagcgggc cgcatcgagg gtgaagatgc cggtgaatac cgagagcctg
     4561 cggagaacta gacgctcatg ctccgacagc agatcataac tccagtccag cgtcgcgcgc
     4621 agcatttggt gccgcggcag cgccgagcgc cgcccctccc agagcagctt gaagcggtcg
     4681 ttcaacagca ccagggtttc ctgaatcccg taggcatcgg cgcggccggc ggcgagttcg
     4741 atcgcaaggg ctattccatc cagccggcgg cagatttcag cgacgacggg cgcatcggca
     4801 tcgttcaatt caaagcggcg gccgctggcg atcgcgcgct cgacgaagag ctgcaccgcc
     4861 gggaaatcca gcgcctgcgc cgcggtgaga ccggcgtcat cgggcggact ccccagcggg
     4921 gacagccgat gaacatgttc gccttcgaca cgcagcgatt cccggctggt cgccagaatg
     4981 tgcacctgcg gtgcctcctc gaaaatgcgt tccgccagcg ccgccgcggt ctcgatcacg
     5041 tgctcgcagc tatcgaggat caacagcatc cgtttgccgc ggaggaaacc tatcagaccg
     5101 cgggttggat ccgcggactg aaccggcagc ccgagcgccg aggccaccgt gcccggaatg
     5161 agaagaggat cgttgagcgg tccaagatca agaaactgaa tggcaccggc aaactcggta
     5221 agcagctcat aacctaccga gaccgcgacg gtggtcttgc cgatgccacc cggtcccacg
     5281 atggtgacga agcgctcggt cttcagttgc tccgatattt cctgaacggt ctggtcgcgc
     5341 cccaccattc gtgtcaggcg ggtcggaaga ttgtgggctt gaccgggctt cgaggccacc
     5401 gtctccaagg gtcttggcgc actcgaacgc gagaccggcg ccacgaaaca atagccctgc
     5461 cccgtaagcg tcgtcagata cctggctcgg actctccatc ccccagtgcc ttgcgaaggg
     5521 cggcgacatg gacccggagg ctaccctcgt cgaccgtgac acccggccag acccgcgcca
     5581 tcagatcgtt cttgctgacc acctcgcccg catgctcgac cagaacaatg aaaatatcga
     5641 gcgcgcggcc gccaagatga agcgggacgc cctccctttc gatcagcctc tgcgccgcgt
     5701 acagccggaa cggtccgaac gaaatgattt catccgtctg cggattttcc ttaattcctt
     5761 ggctcatttc cgaataatac tgcagggcga gccggctcaa gccggtgccg accactgacc
     5821 agcattgcaa cggcagcggg gcgcatcatg cgaaccgttc gactgcgcgc ggcgtccgca
     5881 caggaccagc cgcgcttcga tcactgcgac gtctgtttcc tgagcgccgc cacgtcttgt
     5941 gctgtcatat gagccggctt gctgccgaag cgcgcggtga catagttggc cacggctgca
     6001 atctcgacat cggaataggc attgccgaac gcaggcatcg agaccgcacc ctccggcgtg
     6061 tgtcgcttgg tcccggaaat cacgatctgg gcgacgttgg tcgcaccggg atcgttgacc
     6121 gcccacgcac cggtgagcgt ggcgaagggc gagatcgagc tttcgccgct ccagccgtgg
     6181 caactgacgc aggcgccttc gaacaccatc ttgccgcgtg gatccggcgc gccgccgcca
     6241 tccctgtgcg acgcaggtgc tgatggcgcc agcgtcgccg gcagatcggt cgaggcggtc
     6301 ggcggcacgc tgcgcaaata tgccaccacg gcgcggatgt cttccggcgc tagcttgctc
     6361 aagctgtggt cgaccgcttc acccatcggc cccgaggcgg tgccgtggcc atccgcatgt
     6421 ccgatcgaaa gataggagac cagatcgtcg tcccgccagg cgccgacgcc ggtggtcttg
     6481 tccgagctga tgttgaaggc gcgccagcca gccgtcaccg cgccggcgaa tttcaagcgg
     6541 ttgttgagcg cgaacgccag gtttcgcggc gtatggcatt caccgcaatg ggccagcgcc
     6601 tcggcgagat aagcgcctct gttccattcc gggcttttcg acgtgtcggg ctgaaagcgc
     6661 gtatccggat tgaacagcgc cgaccagaaa cccatcgccc agcgctggtt gaatggaaac
     6721 gtcagcgtat tctcggacgc ggccgcgcgc accggcggca ggctgaacag ataggccttg
     6781 atcgccagcg cgtcggcgtc gcttatataa gtgtaggacg tgaacggcat cgccggataa
     6841 agccgtgcgc catccctgcg gatgccacgg tgcatcgcgg ccagaaaatc ctggtcgctg
     6901 taattgccga tgccggtctc cttgtccggt gtgatgttgg tcgaataaag cgtgccgaac
     6961 ggcagcttga atcccagacc gccggcatat tctttcccgc ctgccgtggt gtggcagacc
     7021 atgcagtccg ccgcccgcgc caaataagcg ccacgctcca cgacgcttgc ctgcgccagc
     7081 gccgccggca cgccggcggg attggcgccg cgatagtccg caagcgccac tttcggacca
     7141 tccgcgaatg ccatcggacc ggggccgcgg acaatccaaa gtccgatcgc caccgcaacc
     7201 accacgatcg cgacaacgct caggagaatg cgacgcgcgg ccgggctcat gcttttttcc
     7261 ctgctgatat aagtgcccgg tcgatcggca gccgccgcaa ggccacaccg gtcgcggcat
     7321 agatggcgtt gcgcaatgcg ggcggcccgg cgttgacgcc ggtctcgccg atgccgccgg
     7381 gcggttcgcc gctcttgacg acatgaacct cgatcttcgg cgtctggtcg atgcggagca
     7441 tccggtagtc atggaagttg gattgctgga cccgtccctt atcgatggtg atttcgccat
     7501 agagtgctgc agtgaggccg aagatcagcc cgccctcgag ctgggccttg atggtatcgg
     7561 gattgacagc gatgccggta tccaccaccg aggtgacgcg acggagctgc acctcgccgt
     7621 gctcgtcgac ttcagcctcg accaccgtcg cgatgaaggt ttcgaacgac ggctgcacgc
     7681 acacgccgcg cccgacgcgc gccggcagcg gctgacccca attggatttt tccgccgcga
     7741 gattgagggc tgccagcaga cgcggctttt tcccgagcat cgagcggcgg aactccaccg
     7801 ggtccttgcc cgccttgcgg gcaagctcgt ccatgaaaca ttcgacggcg aacacgttgt
     7861 tgttgggacc gaccccgcgc cagaatccgg tcggcactgc gggcggttcg gcgcgcacat
     7921 attcgacatg aaaattggga atgtcgtagg gcatgtcggc gccggcatcg atggcgtcga
     7981 tgtcgatgcc tttctggaag gccggcggca gccagcgcgc caggatcgcc gaaccggtga
     8041 cccgatattt ccagccgacg atcttgccgt cggacaaagt cgcggcaatc gtgtcgcggt
     8101 acaccgggcg atagacatcg tgctggatgt cctcttcgcg agtccacacc accttgaccg
     8161 gtccgtcgac ctgcttggcg acgcggaccg ccgcaaccac catgtccggc tcgagcttgc
     8221 ggccgaagcc gccgccgagc agatgattgt tgacgatcac tttctcgacc gcgagtccgg
     8281 ccgctttcgc cgcctccgcc tgcacccggc ttacgatctg cgtgccggtc cagatttcac
     8341 aggagtcggg cttgaaatga accgtggcgt ttaacggctc catcgtggca tgggcgagaa
     8401 acggaagctc gaatgcggct tcgagttttt cgccggtagc taaccccttg gcgatgtcgc
     8461 caatggattt ggcgaccgcg ccgtccttct cgctggccgt gcgcagatcc tgccagatgt
     8521 cctttgagct gatgcgcgcg ttcggacctt cattccaggt caccttgaga gcatcgagcc
     8581 ccttcttggc cgcccacatg tgatcgccga ccacggcgac catatcgtcg agcacgacaa
     8641 tctgctgcac gcccgggatt ttcctggcgg cgctgtcatc gaccttg
//
LOCUS       DATNOI010000462        18372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1083815,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000462 DATNOI010000000
VERSION     DATNOI010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18372
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083815"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VN968_18385"
     CDS             complement(<1..386)
                     /locus_tag="VN968_18385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXL31239.1"
                     /translation="MYIVTSSHPPGPPTPALMEAMGKLADREIKAGRMIDSGGLMPVA
                     MGAQVRITDGQLRIVDGPFVETKEVIGGYAIFELRDKEEAVAAAVEFMQLHKDHMPGW
                     EGTCEVRSFAMPAHEGGCQVDALAHA"
     gene            complement(515..835)
                     /locus_tag="VN968_18390"
     CDS             complement(515..835)
                     /locus_tag="VN968_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31240.1"
                     /translation="MVSNIDDDLLALTPLKRNFDLVCTAQRAEGYKPDGTLFRYLIEN
                     AGCAKHEILHSGQSQFTDLVGGKPLGLTIAWINRRQVALDASVPRPDFILPDIQSLRD
                     LVDL"
     gene            complement(799..1161)
                     /locus_tag="VN968_18395"
     CDS             complement(799..1161)
                     /locus_tag="VN968_18395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31241.1"
                     /translation="MSFDVFGTLISVRDSSYGAFAQILADAGAHHLDVKAFWECWEHR
                     NIAHYWDPYRSYRDICELSLAETFAHFETTGDSRLIDRYFDAFPDFFLYDDVVRTLDR
                     LSRRYWRWFRILTTTSLR"
     gene            complement(1504..1914)
                     /locus_tag="VN968_18400"
     CDS             complement(1504..1914)
                     /locus_tag="VN968_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXL31242.1"
                     /translation="MPYAGGCSCGAVHYELTAEPIRGFQCQCRDCQRDTGSGHASVMV
                     FPRAALRITGPVAEISRTADTGAQKLKGFCGSCGSPLYNKPLSKPDMIGIYVGTLDDP
                     SGFKPQAVIFASRGHGWDHLDPALPKLPNMRPSP"
     gene            complement(1970..2398)
                     /locus_tag="VN968_18405"
     CDS             complement(1970..2398)
                     /locus_tag="VN968_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXL31243.1"
                     /translation="MLDTRPSLILKRHIKAAPAKVYAAWTDPEKIMRWFGSDQGPTLH
                     AETDLRPGGRFRFAFRMMDGEERDVSGAYREVLPGRKLSFTWNWANPCEPESLVTIDL
                     EPDGDGTLLTLTHEQIAGDTVHDYRRGWIGALDKLEKQFA"
     gene            complement(2534..3226)
                     /locus_tag="VN968_18410"
     CDS             complement(2534..3226)
                     /locus_tag="VN968_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AprI/Inh family metalloprotease inhibitor"
                     /protein_id="HXL31244.1"
                     /translation="MSGRLGIALATLASIACGSQAWPQDAATLKKNMIGQWELSTTER
                     SKTCVVTLKSDTTPQGLKLELEPGCAAALPFTKDITAWNVKGLDIVRLQDAAGQPVID
                     FTEVESGILEGMRTNEGIYILQNLAAARSLAKSMDQMIGDWAMVRGNGQAICGLTLTN
                     NEATPDNFEVFLKPKCDPAIAAFAPTQWRLDHGQMVLMSAKGETWQFEADDNAQWRRV
                     PDSADPLIMLRQ"
     gene            complement(3226..4215)
                     /locus_tag="VN968_18415"
     CDS             complement(3226..4215)
                     /locus_tag="VN968_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HXL31245.1"
                     /translation="MVAGNISSEKIDLLIYGPSKPIVNNGFSDQFVLHSFETSHDLDR
                     LTPAVAGKIRGAAVTYNSVRGDGTTLARFPKLEIVSSFGVGYDHIDANHAREHNIIVT
                     NTPDVLTEEVADIAMGLLIATLREFVKADRYLRSGLWLTQQFPLSVGSLRDRKVGMVG
                     MGRIGQAIGRRLDASRVPVVYHSRNPAAGVSYKHYPDLIEMAKAVDTLILILPGGAST
                     AKMINADVMKALGPRGVIINVARGSVVDEPALIAALKSGTILAAGLDVFANEPTVPDE
                     LRTMQNVVLLPHIGSASVVTRNAMDQLVVDNLKAWFSGKPPLTPVAETPVKGR"
     gene            complement(4215..5051)
                     /locus_tag="VN968_18420"
     CDS             complement(4215..5051)
                     /locus_tag="VN968_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011082921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL31246.1"
                     /translation="MSSLQTPPLSAGQGGPESRALRVVRILLPVAVLAAGIAGWELVV
                     RVNDIPPYVLPGPSAVFQTLLSDWPVLSQSLLVTLLTTLEGFAAAAFGGIALALVFNQ
                     SKWLEYSLFPYAVILQVTPVIAIAPLLLIYLSQQSAVIVCAWIVGFFPVLSNTTLGLN
                     SVDRNLAGLFRLYGASRIQTLAYLKLPSALPFILGGLRIAGGLSLIGAVVAEIAAGSA
                     GAGSGLAYRIAESGYRLNIARMFAALLLLSAAGIVIYVLLALTSHLVLRRWHESALGK
                     DN"
     gene            complement(5048..5854)
                     /locus_tag="VN968_18425"
     CDS             complement(5048..5854)
                     /locus_tag="VN968_18425"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31247.1"
                     /translation="MVGPAALSEAVSGTEGLAVSLRGVTKIYDSGVMALGPFDLDVNK
                     GEFVSLLGPSGCGKSTALRLIAGLSAPTSGSVGIAKPAGQARPGHSVGFVFQEPTLMP
                     WTSVRENVRLPLKLAHAPVAEANARIDQALTQVGLTEFADAYPRELSGGMKMRVSLAR
                     ALVTDPDILLMDEPFAALDEITRFRLNNDLLALWRNLRKTIIFVTHSVFESVYLSQRV
                     IVMTPRPGRLSAQFGIDTLEPRGEDFRTSAEYAGYCRKVSGALAPEKPGS"
     gene            complement(5872..6894)
                     /locus_tag="VN968_18430"
     CDS             complement(5872..6894)
                     /locus_tag="VN968_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31248.1"
                     /translation="MNPVYLLRALTAGLVAALVALSPAGAQTALDKVSFGTNWVAEAE
                     HGGFFQALADGTYKKYGLDVTIVPGGPNDNNRMLLIAGKLDFFMAANSLMSFDAAAND
                     VPVVTVAAIFQKDPQVFLTHPESKVAKLEELKPLTLFVSKEGISSYFQWLKSEYGFSE
                     EKVKPYTFNSQPFLANRLSAMQGYVTSEPFAIEKAAKFKPGIILLADYGFNTYSTLIE
                     TRREFIDKKPDLVQRFVDASIVGWYNYLYGDNAPANAMIKKLNPEMTDDLLAYSVAKM
                     KEYGIVDSGDALRDGIGAMSDARVASFFDKMVRAGVVRRDIDFRKSYTLRFANKGVGL
                     DLRPKN"
     gene            7012..7827
                     /locus_tag="VN968_18435"
     CDS             7012..7827
                     /locus_tag="VN968_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HXL31249.1"
                     /translation="MTSTVPSRDWTEIHWPDIAGAEPARWIAVLPLAATEQHGPHLPV
                     GTDVMITQAYLARVRELLSDSIPATFLPLQRVGISTEHIGYPGTLTLPTEVALTTWMA
                     LGECVARAGIKKLVMVTSHGGNSAAMSLVAQDLRARCGLLAVTTGWSRLSAPEGLFSA
                     DELRHGIHGGAVETSIMLARYPQHVRKDHIADFRPASIAMEQDYRWLSAHRPAPFAWQ
                     AQDLHPSGAAGDATLASAEKGEHLLDHGARAFCELLADVDKFDPATLSGGPTG"
     gene            7976..8239
                     /locus_tag="VN968_18440"
     CDS             7976..8239
                     /locus_tag="VN968_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31250.1"
                     /translation="MSIKTRIAALALAALAVTGSIATTTSPAEARGLGWGIGAGLVGA
                     AVVGSAIAASNDGYYYGGYRRCGWVRQFDAYGNYLGRVRTCNY"
     gene            complement(8424..9266)
                     /locus_tag="VN968_18445"
     CDS             complement(8424..9266)
                     /locus_tag="VN968_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HXL31251.1"
                     /translation="MRWCVSIGAALVAGAVAGADVAGIAAPSPQSQQTESKDNLPSVD
                     IELVIAVDVSYSMDLDELAVQREGYAQAIVSKDFLQAMKTGPNRKVAVTYFEWSASSD
                     QKIIIPWRVIDGPESADAVAAEIMKTPVRRGSRTSISGAIDFAMPLFEENPYRGLRRV
                     IDISGDGPNNNGAPVTGARDAALAKGITINGLPIMVKEPSYSTMDIDNLDFYYEDCVI
                     GGPGSFVVSIKDRDRFKEAIRTKLLLEVAGRTPEQRIVPVAEKEPRVPCLIGEKIWQD
                     RWGR"
     gene            9546..9824
                     /locus_tag="VN968_18450"
     CDS             9546..9824
                     /locus_tag="VN968_18450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="HXL31252.1"
                     /translation="MAHTIREKNKLLARVRRIRGQVEAIERALAEEAECERIMHMIAG
                     IRGGVAGLMAEVVEDHIRTHLVDPEKNPGALNADAADQLIEVVHTYLK"
     gene            9833..11119
                     /gene="dmeF"
                     /locus_tag="VN968_18455"
     CDS             9833..11119
                     /gene="dmeF"
                     /locus_tag="VN968_18455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HXL31253.1"
                     /translation="MTNAETIFQFPPHDHVFLGEDHDRAERRTWAVIILCSVMMIAEI
                     IGGALFGSLALIADGLHMSTHAGALLLAALAYTYARKYANDRAFTFGTGKFGDLAGYS
                     SAIVLAMIALLISYEAVSRLLTPVPISFNEAIPIAVLGLVVNVASAWLLSGGHHHGHS
                     HGHSQGHDHDHDGDEPRRIELGTGAVEIEVFEDNVPPRFRVRADTGVLLAAPDVTIET
                     IRPDGGCQLFAFEDRGGYLESRDEIPVPHAFLAKFRLVQGAQHHDHELAFEEHDHDHH
                     HQATHGAHHRDNNMRAAVIHVMADAAVSVLVIVGLLLARTFGWLWMDPLAGFIGALVI
                     ANWSVGLLRDTGGILLDRTPDPRMAEKVRMVIESEGDQVTDLHLWRLGPGHLGVIVSV
                     ATSGAREAAHYRQRLAKFADLSHVTVEVQHCHSTNG"
     gene            11361..12062
                     /locus_tag="VN968_18460"
     CDS             11361..12062
                     /locus_tag="VN968_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HXL31254.1"
                     /translation="MNASIDQAAISLGTATLMGACIGFERQWRQRMAGLRTNTLVAIG
                     AASFVIFEGLFPDDGSPTRVAAQVVSGIGFLGAGIIFREGLHVTGLNTAATLWCSAAV
                     GLLAGSGHPRHAALATAFVVLVNLTLRPLVRIINRQPISQAETDFHYRVRVVCRNPEE
                     AHIRALLLQSTSNGQLSLRRLDSTDLEESGRVEVTAQLTSQSKSDTVLEQVVGRLSLE
                     PTVSAASWSVGPILE"
     gene            12403..14367
                     /locus_tag="VN968_18465"
     CDS             12403..14367
                     /locus_tag="VN968_18465"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HXL31255.1"
                     /translation="MMLAGVGPTNSAEYSVSRPTKSLAPVPFLNWTGFYVGGHLGYGR
                     GHASGSMTDAVPTISSNSFGSLFGGLQLGYNHLLTSGFLVGVESDVSFANFLGADDIV
                     RSRFTAQGNLAEKIDYIGRLRGRLGYASGDWLFYGTGGFAWSQARFIETPGLIADEDK
                     VLRTRPGWVLGAGAEVAIAPDWSARLEYLYDNFGRVDATLPSGRRSDSTFDTHTLRLG
                     LNWHLGRAASESPSIENRAYPVIAPDNWNVHGQYTFVGQGYPSFRSPFEGPNSLTGSK
                     QAQNTMSATAFVGLRLWEGAEFYINPELMQGFGLSNVHGLAGFSNGEAQKSSFALPRM
                     NVARIFIRQTFGLGGEQETISDGPNQLAGKQDISRITVTAGKFAVTDAFDGNVYANDP
                     RTTFLNWNIYGGGSYDWTMDKLSWTWGAFVDFNQKDWAFRTGYFLLPVVSNSNNFDTH
                     IPDRGQYTAELELRYSLFSQPGKLRFFGWLNHGTMGGYSDALALPPTSPDYPDITLTR
                     RIRTNYGLVANIEQAISPDLGMFSRATWSPGRTEILGWTDCSESLSFGTVLNGTSWGR
                     PDDKIGVAGVVEGLSAESRAYFAAGGLGILIGDGQLNYSREKIIEAYYAYSLNKWATL
                     TFDYQFINNPGYNADRGPASVFSGRFHAEF"
     gene            complement(14405..15697)
                     /locus_tag="VN968_18470"
     CDS             complement(14405..15697)
                     /locus_tag="VN968_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXL31256.1"
                     /translation="MLAVELGIVTILIIVNGLLSMSELAIVSSRPARLAGLVEKGVKG
                     SRRALALASDPGKFLSTVQIGITLIGVLSGAFSGATLGLRLTAWLVEFGLSQGLADAI
                     GVGAVVTIITYASLIVGELVPKQIALRDPEAVAVRVAPHMVLLSKISLPLVWLLDRSG
                     KALLWLLGQRGETEEKVSEEEIRTLVVEAENAGVLEPGEKEMIAGVMRLGDLPVGAVM
                     TPRHEVSMINLTDAPEVIKATLANRAHSRFVVFERDRDAAVGIIQAKDLLDTYLSGQT
                     PDARKLVRDAPIIPESLDARDVVSILRDSPVHMGLVHDEYGTFQGVVTSADILEAIVG
                     SFHTEEGPAEPAAVKRDDGSYLISGWMPAIEFAQLLKVPLPASRQYQTIAGFLLQQFG
                     TIPNVGEHVDALGWRFEIVDLDGRRIDKVLATPISEPK"
     gene            16124..18109
                     /locus_tag="VN968_18475"
     CDS             16124..18109
                     /locus_tag="VN968_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HXL31257.1"
                     /translation="MIRSSRSWVCIFAGVGLSLLVVDGSAIAADMPVKAPYIRSVFDW
                     TGLYVGAHAGFGRGSSTAVLADPATSTTSNSFGGMIGGVQAGYNYQLPTGMLLGLEAD
                     ISFPNYLTSNSVVSSLATIRSDITEQWDYVGTARGRVGYVAGPWLVYATGGLAWAGER
                     FLNTPAIGSDEKTLHTRLGWAAGGGVEYAFAPHWSARLEYLYSQFGKADIAFPSATQY
                     TSSLDFQSLRIGLNRKIDWPGSGNFSPASLTDPESDRWEIHGQSTYLPQGYPAFRALY
                     TGPNSLTPAPQVQATWSNSLYLNARLWEGGEVYYNPELLQGFGLSDTVGVAGFPSGES
                     QKSNFPYPHYNTSRLFLRQTFGFGGEQEEWTSGPSQLAGKVDVSRLTILVGKLAVTDI
                     FDGNAYAKDTRKDFMNWSMWAPGAFDYSADKLGLTYGATAEFNQKQWALRSGYFLVDA
                     VSNSNNFDTKLFQRGEYVVELETRYSLFNQPGKLRTIAWLNSVFSGSYRETLDNPALN
                     LDIAQTRTGRIKYGYVINLEQALTDEVGLFGRWSWNDGKTEIMAFTDIDASLSLGVSI
                     KGTKWGRPDDVIGIGGAINALSRDHRDFLAAGGLGPLIGDGQLNYQPERILESYYAYA
                     LNKQLTLTGDYQLITNPAYNADRGPVHVFSGRLHAEF"
     gene            complement(18115..>18372)
                     /locus_tag="VN968_18480"
     CDS             complement(18115..>18372)
                     /locus_tag="VN968_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HXL31258.1"
                     /translation="RMTEATFAVEKKVTERASLFVEYVGDYPENAGPSQLLNSGGLYH
                     LSPVQQVDFHVAFGLNHNAPSYVVGVGYSFRFDGLFAGKPR"
BASE COUNT         3676 a         5607 c         5459 g         3630 t
ORIGIN      
        1 caggcgtggg cgagcgcgtc gacctgacag cctccttcat gggccggcat ggcgaacgag
       61 cgaacctcgc acgtcccttc ccagcccggc atgtggtcct tatggagttg catgaactcg
      121 accgccgcgg ccacggcctc ctccttgtct ctgagctcga agatcgcgta gccgccgatc
      181 acctccttcg tctccacgaa cgggccatcg acgatccgga gctgtccgtc cgtgatgcgc
      241 acctgggcac ccatggcgac cggcatcagc ccgccgctgt cgatcatgcg gccagccttg
      301 atctccctgt cggcgagctt gcccatcgcc tccatcagtg ccggcgtcgg agggccagga
      361 gggtgggacg atgtgacgat atacatgaag cgcatcctag gaactcctct tgaggcgagc
      421 cgcaggcacc gagcccttcg gaactgtctc gctatccagg acgacgaacc agcccaggga
      481 gaatcgacat gcgccttgaa aaaattgtgc cggcttacag atcgacaaga tcgcgcagag
      541 attgaatgtc cggaaggatg aaatccggac gcggaacgga ggcgtcgagg gcgacctggc
      601 ggcggttgat ccaggcaatg gttagtccaa gcggtttgcc gccgacgaga tcggtgaatt
      661 gcgattgccc ggagtgcagg atctcatgct tcgcacagcc cgcattctcg atcagatagc
      721 gaaacagcgt tccatcgggc ttgtagccct cggctcgctg tgccgtgcat accaggtcga
      781 agtttcgctt caatggcgtc agcgcaagga ggtcgtcgtc aatattcgaa accaccgcca
      841 gtatcgacga gacaaccgat caagcgtgcg gacgacatcg tcatagagaa agaagtccgg
      901 aaaggcatca aagtagcgat caatcagccg gctgtcgccc gtcgtctcga aatgcgcgaa
      961 tgtctcggcg agtgagagtt cgcaaatgtc tcgatacgac cggtacgggt cccaataatg
     1021 cgcgatattc cgatgttccc aacattccca gaacgccttg acgtcgagat ggtgcgcgcc
     1081 ggcatcggca aggatttgtg cgaaggctcc gtaggagctg tcgcgaacac tgatcaacgt
     1141 gccgaagacg tcgaatgaca tcattatcgg ccgcagacgt gcgcggtgcg ttgcatggct
     1201 catcgatcga acccccgacg attgccattc cgtaagcgct actggagtta cattcatggt
     1261 atctccgttc gaaattgcga acgtctcgat tgcaaaccgg taggctaccc ttgttcgtgg
     1321 gataaatcat aagtcggcgg acaagccaac cggtctggca gcccgcttcc tatcttccct
     1381 ccggcgtcct cttcgaactg caatctccca tcgaggcgac ctccagccgg ctttcaccga
     1441 cgccggtgtc gctccggatc gccaattgga tgatgtcgac cggcaagtga agtgccgcga
     1501 cggctatggc gagggccgca tgttcgggag cttgggcagc gccggatcca ggtgatccca
     1561 gccatgcccg cgcgatgcga agatcaccgc ctgcggcttg aagccggatg gatcatcgag
     1621 agtgccaaca tagatgccga tcatgtccgg cttggacagg ggtttgttgt aaagcggcga
     1681 gccgcaagac ccgcagaagc ctttcagctt ttgcgcgccg gtatccgcgg ttcgtgaaat
     1741 ttcggcgaca gggccggtga tgcgcagggc cgcgcgcggg aacaccatga ccgacgcgtg
     1801 cccggaaccg gtatcccgtt ggcagtcgcg gcattgacac tggaagcctc gaatgggctc
     1861 ggcggtgagc tcataatgga ctgcgccaca ggaacagccg cccgcgtacg gcatcacgcg
     1921 aacctcctga tctccatacc gaactgcatc gagttcccct cctctgaaat caggcgaact
     1981 gcttttcgag cttgtcgagt gcaccgatcc agccccggcg gtagtcatgc acggtgtcgc
     2041 ctgcgatttg ctcgtgagtc aaagtcaaca acgtcccgtc gccatccggc tcgagatcga
     2101 tcgtcaccag cgattcgggc tcgcatgggt tggcccagtt ccaggtaaag ctcagcttcc
     2161 ggccgggcag cacctcgcga taggcgccgc tgacgtcgcg ctcctcgccg tccatcattc
     2221 gaaaggcgaa gcgaaagcgc ccgcctggcc gcaagtccgt ctcggcatgc aacgttggcc
     2281 cctgatcgga cccgaaccag cgcatgatct tttccgggtc cgtccatgcg gcgtagactt
     2341 tcgccggcgc cgccttgatg tggcgcttga ggatgaggct gggcctggta tcgagcatgg
     2401 cggtcaagcg ctctcataat acttaaccat gtggtaaagt atatagcgac actcgcgcca
     2461 gtcaagcgcg ttctaaaaag cgatggcgga cgacgatacc caacgccgcc gcgccgaagc
     2521 tatcgtctga aattcactgg cgcagcataa tcagcggatc ggcgctgtcg ggcacccgcc
     2581 gccattgcgc gttgtcgtcg gcctcgaact gccaggtttc gcccttggcc gacatcagca
     2641 ccatctggcc gtggtcaagc cgccattgcg tcggcgcgaa cgcggcgatc gccggatcgc
     2701 atttcggctt gaggaatacc tcaaaattat ccggggtcgc ttcattgttg gtgagcgtca
     2761 ggccgcagat cgcctggccg ttgccgcgca ccatcgccca gtcgccgatc atctgatcca
     2821 tcgatttggc aagcgagcgc gcggccgcga ggttctgcag gatatagatg ccctcgttgg
     2881 tccgcattcc ctccagaatc ccgctctcga cttcggtaaa atcgatcacc ggctgtcccg
     2941 cggcatcctg cagccgcacg atatccagcc ctttgacgtt ccaggcggtg atatccttgg
     3001 tgaagggcag cgccgccgcg cagccgggct cgagttcaag cttgaggcct tgcggcgtgg
     3061 tgtcgctctt cagcgtgacg acgcaggtct tgctgcgttc ggtggtcgag agctcccact
     3121 ggccgatcat gttttttttc aaggtcgcgg catcctgcgg ccaggcctgc gaaccgcaag
     3181 cgatcgacgc cagcgtcgca agcgcaatgc caaggcgacc cgacatcagc ggcccttcac
     3241 cggggtttcg gcaaccggcg tcagcggcgg ttttcccgaa aaccatgcct tcaggttgtc
     3301 gaccaccagc tgatccatgg cgttccgggt caccaccgaa gccgagccga tgtgcggcag
     3361 cagcaccacg ttctgcatgg tgcgcaattc atccggcacc gtcggttcgt tggcgaacac
     3421 atcgagcccg gcggcaagga tggtccccga cttcagcgcg gcgatcaggg ccggctcatc
     3481 caccaccgag ccgcgcgcca cgttgatgat cacgccgcgc ggcccgagcg ccttcatgac
     3541 gtcggcgttg atcatcttcg ccgttgaagc gccgccggga aggatcaaga tcagggtatc
     3601 gaccgccttc gccatctcga tcagatcggg ataatgcttg tacgacaccc cggccgccgg
     3661 gttgcgcgaa tgatagacca cgggaacgcg ggacgcatcg agccggcgcc cgatcgcctg
     3721 gccgatccgg cccatgccga ccatcccgac cttgcggtcg cggagcgagc cgacgctcaa
     3781 gggaaattgc tgcgtcaacc agaggccgga acgcaaataa cggtcggcct tgacgaactc
     3841 gcgcaaggtc gcgatcaaaa gtcccatggc gatatccgcg acctcttcgg tcagcacgtc
     3901 cggcgtgttg gtgacgatga tattgtgctc acgggcgtga ttggcatcga tgtgatcgta
     3961 gccgacgccg aacgaggaga cgatctcgag cttgggaaag cgcgccagcg tcgtgccgtc
     4021 gccccgtacc gaattgtagg tcaccgcggc gccgcggatt ttgccggcga cggccggggt
     4081 cagccgatcg aggtcgtggc tggtttcaaa cgaatgcagg acgaactggt cggaaaaacc
     4141 gttgttgacg attggcttgc tcggcccata gatcagcaga tcgatctttt cggaagagat
     4201 attaccggca accatcagtt gtcctttcca agggcgctct cgtgccagcg ccgcaatacc
     4261 aggtgcgagg ttagcgccag cagcacgtaa atgacaatcc cggcggccga cagcaacagc
     4321 aacgcggcga acattctggc aatgttgagg cggtagccgg attccgcgat tcgataggcc
     4381 aatccggagc ctgcgccggc cgaccccgcg gcgatctcgg cgaccacggc gccgatcagc
     4441 gaaagtccgc ccgctatccg caatccgcca aggataaatg gcaacgccga gggcagtttg
     4501 agataggcca gcgtctgtat ccgcgaggcg ccgtaaagcc gaaacaggcc ggccagattg
     4561 cgatccacgg aattgaggcc gagcgtggtg ttggacagca ccggaaaaaa tccgacgatc
     4621 caggcgcaga cgatgacggc gctctgctgc gaaagataga tcaacagcag cggcgcgatc
     4681 gcgatcaccg gcgtgacctg caggatcacc gcatagggaa acagcgaata ttccagccac
     4741 ttcgactggt tgaacaccag cgccaaggcg atgccgccga aggcggcggc ggcgaatcct
     4801 tcgagcgtcg tcaataacgt gaccagcagc gattgcgata gcaccggcca gtcgctgagg
     4861 agggtctgga aaaccgccga tgggcccggc agcacatagg gcggaatatc gttgacgcgc
     4921 accacgagct cccaacccgc gatgccggcc gcaagcacgg cgaccggaag caggattcgg
     4981 acaacacgca acgcgcgact ctccggcccg ccctgtcccg cggataatgg cggtgtttgc
     5041 aatgagctca tgaaccgggc ttttcgggcg ccagtgcgcc ggaaaccttg cggcaatagc
     5101 cggcatattc ggccgaggtg cgaaaatcct ctccgcgtgg ctcgagcgta tcgataccaa
     5161 actgcgcact gaggcgtccg ggccgcggtg tcatcacgat cacgcgctgc gacaaataca
     5221 ccgactcgaa taccgaatgc gtgacgaaga tgatggtctt gcgcagattg cgccacagcg
     5281 caagcagatc gttgttgagc cggaaacggg tgatctcgtc gagcgcggca aacggttcat
     5341 ccattaagag gatgtcggga tcggtcacca atgcgcgcgc cagcgagacc cgcatcttca
     5401 tgccgccgga taattcacgc gggtaggcgt cggcaaattc ggtcaacccc acctgcgtca
     5461 gcgcctgatc gatgcgcgcg ttcgcctcgg cgaccggcgc atgcgcgagt ttcagcggca
     5521 accgcacatt ttcgcgcacg ctggtccacg gcatcagcgt cggctcctga aacacaaagc
     5581 cgacggaatg tcccgggcgc gcctgaccgg cgggtttggc aatgccgacg ctgccggagg
     5641 tcggtgcgct gaggcctgcg atcagcctca gggcggtaga ctttccgcag cccgacggtc
     5701 cgagcaggga aacgaactcg cccttgttca cgtcgaggtc aaatggtccc agtgccatca
     5761 cgccgctgtc atagatcttg gtgacgccgc gcaggcttac cgccagaccc tcagtaccgg
     5821 aaacggcttc ggacaaggct gcgggcccga ccatcacggc tccagttctt tctagttctt
     5881 gggccgcagg tccaggccga cccccttatt ggcaaagcgc agcgtgtacg acttgcggaa
     5941 gtcgatgtcg cggcggacca cgccggcccg caccatcttg tcgaagaagc ttgcgaccct
     6001 cgcatcgctc atggcgccga taccgtcgcg caacgcgtcg ccggaatcga caatgccgta
     6061 ttccttcatc ttggcgacgg agtaagccag caagtcgtcg gtcatttccg gattgagctt
     6121 cttgatcatc gcgttggcgg gcgcgttgtc gccgtaaagg taattgtacc aaccgacgat
     6181 cgaggcgtcg acaaagcgct gtaccaggtc cggcttcttg tcgatgaact cgcgacgggt
     6241 ttcgatcagg gtagagtagg tgttgaagcc atagtctgcc agcaagatga tccccggctt
     6301 gaatttggcc gccttttcga tcgcgaacgg ctccgaggtg acatagccct gcatcgcgct
     6361 caggcggttg gcgagaaacg gctgtgagtt gaacgtatag ggcttgacct tttcctcgct
     6421 gaatccgtat tcggacttca gccattgaaa atagcttgag atcccttcct tggacacgaa
     6481 cagtgtcagc ggcttcagct cttccagttt ggcgaccttc gattccggat gcgtcaggaa
     6541 aacctgtgga tccttctgga agatggcggc gacggtcacc accggcacgt cgttggcggc
     6601 cgcatcgaat gacatcaggc tgttcgcggc catgaagaag tcgagcttgc cggcgatcag
     6661 cagcatgcgg ttgttgtcgt tgggaccgcc tggcacgatg gtgacgtcga gcccatattt
     6721 cttgtaggtg ccatcggcca gggcctggaa aaatccgccg tgctcggcct cggcgaccca
     6781 attggttccg aacgaaacct tgtccagcgc ggtttgggca cccgccggcg agagggcgac
     6841 gagggcggcc acgaggccgg cggttaacgc tcgcagcaaa tagaccgggt tcatgattgg
     6901 actccgtcga tcgttttggc ccatgatggg aagctggacg acgttggtac gggttcgcca
     6961 ggaatgaatg caggctaccc tgcaacttga cttaatgaaa tgtctcgctc gatgacttca
     7021 actgttccct cccgcgactg gaccgaaatc cactggcctg acatcgccgg agccgagccg
     7081 gcgcgatgga tcgccgtcct gccgctggca gccaccgagc agcacggtcc gcatttgcct
     7141 gtcggtaccg atgtcatgat cacgcaggcc taccttgccc gggtgcgcga gcttctctcc
     7201 gacagcattc cagcgacgtt ccttccgctt cagcgggtcg ggatttcgac cgaacacatt
     7261 ggctatccgg gcacgctgac gctaccgacc gaggttgcgc tgacgacctg gatggcgctt
     7321 ggcgaatgcg tcgcccgcgc tggcatcaaa aagctggtca tggtgacaag tcatggtggc
     7381 aatagcgcgg ccatgagcct ggtggcgcag gacctgcgcg cgcgatgcgg gcttctggcc
     7441 gtcaccacgg gatggtcgcg cctcagcgcg ccggagggat tgttttcggc ggatgaattg
     7501 cgtcacggca tccatggcgg cgcggtcgag acctcgatca tgctggcgcg ctatccgcag
     7561 cacgtgcgca aggaccacat cgccgatttc aggcccgcca gcattgcgat ggagcaggat
     7621 tatcgctggc tttcggcgca ccggccggcg ccgttcgcct ggcaggccca ggaccttcat
     7681 ccaagcggcg ccgcggggga tgcgaccctg gcctctgcgg aaaaaggcga gcacctgctc
     7741 gatcacggcg cccgcgcgtt ctgcgaattg ctcgccgatg tcgataaatt tgatccggca
     7801 accctttcgg gcggcccgac gggctgacgt atcgccatat aaccatctgt aataattata
     7861 gaatatcgtc atcacgaaat ttcgaccgcc cgattcgaac cggaatcgaa gacgcttcgt
     7921 ccaactggca cgtgcggacc acaacggacc gcttttcagc cggatggagt ttttcatgtc
     7981 gatcaaaacc agaattgcag ctcttgctct cgcggctctc gccgtcaccg gcagcatcgc
     8041 aaccaccacc agcccggcag aggccagggg cctgggctgg ggcatcggcg ccggtctcgt
     8101 cggcgccgcc gtggtcggca gcgcgatcgc cgccagcaac gatggctatt attatggcgg
     8161 ctatcgccgc tgcggctggg ttcgccagtt cgatgcctac ggcaattacc tcggccgcgt
     8221 ccgcacctgc aactactgat gatcgactga cggaatcctg gattgcgcgt ccgacacggg
     8281 cgcctcatcc accccgtgtc gtacccgacc cgcccggttg tccccccggg cgggtcatcc
     8341 ttcttggatt cccgaacgac tttatggctt gggctgcaac agagctgcgc tagcctgctg
     8401 gtgctcgcgg acgacccccg ggatcaccgc ccccagcggt cctgccatat tttctcaccg
     8461 atcagacaag gcacccgcgg ctctttctcg gccaccggga cgatccgctg ctccggcgtc
     8521 cggcctgcga cttcgagcag gagcttggtg cggatcgcct ccttgaacct gtcacggtcc
     8581 ttgatcgaca ccacgaacga gcccggccct ccaatcacgc aatcctcgta gtaaaaatcg
     8641 aggttatcga tgtccatcgt cgaataggac ggctccttca ccatgatcgg caggccgttg
     8701 atggtgatgc ccttcgccag cgccgcgtcg cgcgcgccgg tcacgggtgc accgttattg
     8761 ttcggaccat cgccggaaat atcgatgacg cgccgcaggc cccgataggg gttttcttcg
     8821 aacaatggca ttgcgaaatc gatcgcaccg gaaatcgacg tgcgcgagcc ccgacgaacc
     8881 ggcgttttca tgatttcggc agcgacggcg tcggccgatt ccggaccatc aatcactcgc
     8941 cagggaatga tgatcttctg gtcgctcgat gccgaccact cgaaatacgt caccgccacc
     9001 ttgcggttcg gaccggtttt catcgcctga aggaaatctt tcgagacgat cgcctgtgca
     9061 tagccctcgc gctgaacggc aagttcgtcc agatccatcg agtaggaaac atcgacggcg
     9121 atgacgagtt cgatatcgac ggagggcaaa ttgtctttgc tctcggtctg ttgcgactgc
     9181 gggctcggcg cggcaatgcc tgcgacgtca gctccggcta ccgctcctgc gaccagcgcc
     9241 gccccgattg agacacacca gcgcatagcg gccctcccat cgtttcaagg caatggtgac
     9301 atgcatgacg cgctgcgcaa ggcaggaacc cgggttttgc ttcacattcc ggttagtcgc
     9361 gtcggattgg cggaatttac cggcagcgcg gaatcgctag accagcgctc cgaggcgttg
     9421 aagagttcag tcctcaccac ctgcattccg gcttgctgat cggttcggcg acaaagaagg
     9481 tcttcgcgat catcggcctc aaccgcggtt gacctagcat aggggggtac cctatatgaa
     9541 ggcagatggc acatacgatc agagagaaga acaagctgct cgcccgggtc cgccgtattc
     9601 gcggccaggt cgaagcgatc gagcgtgcgt tggccgaaga ggccgaatgc gagcgcatca
     9661 tgcatatgat cgcgggcatt cgcggcggcg tcgcgggcct gatggccgaa gtggtcgaag
     9721 accacatccg cacccatctg gtcgatccgg agaagaatcc gggcgcgctt aacgccgacg
     9781 cggcggacca gctcatcgaa gtggtccaca cctatctcaa gtgagggaca gcatgaccaa
     9841 cgccgaaacg atctttcagt ttcctccgca tgaccacgtg tttctgggcg aggatcacga
     9901 cagagcggag cgccggacct gggcggtgat catcctttgc agcgtcatga tgatcgctga
     9961 aattatcggc ggtgctctgt tcggctcgtt ggcgctgatt gccgacggat tgcacatgtc
    10021 gacccatgcc ggggcgctgc tgctggccgc gctggcttat acctatgccc ggaagtatgc
    10081 gaacgatcgg gctttcacgt tcggcaccgg caagttcggc gatctggcgg gctactccag
    10141 cgccatcgtt ctggcgatga ttgccctcct gatcagctat gaggccgtgt cgcggctgct
    10201 caccccggtt cccatcagct tcaacgaggc gatcccgatt gcagtgctgg gccttgtggt
    10261 caatgttgcg agcgcgtggt tattgtccgg cggacatcat cacggccaca gccatggtca
    10321 ctcgcagggt catgaccatg atcatgatgg tgatgagccc cggcgcatcg agttgggcac
    10381 cggcgccgtc gaaatcgagg tttttgaaga caacgtgccg ccgcgttttc gcgtgagggc
    10441 agacaccggt gtattgctgg cggcccccga tgtcaccatc gagaccatca gaccggatgg
    10501 cggctgccag cttttcgcgt tcgaagaccg cggcggatat ctggaatcgc gcgacgagat
    10561 tccggtgccg catgccttcc ttgccaaatt tcgcctcgtg cagggggccc aacaccacga
    10621 ccacgaactg gcattcgaag agcacgatca cgatcaccat caccaggcca cgcatggcgc
    10681 gcatcaccgc gacaacaata tgcgggccgc cgtcattcat gtgatggctg atgccgcggt
    10741 atcggttttg gtgattgtgg gactgctgtt ggcgcgcaca ttcggctggc tctggatgga
    10801 cccgcttgcc ggattcatcg gcgcgctggt tatcgccaac tggtccgttg ggttgctgcg
    10861 cgataccggc ggcatcctgc tcgatcgcac gcccgatccg cgcatggcgg agaaagttcg
    10921 catggtgatc gaaagcgagg gggatcaggt taccgacctg catctttggc gacttgggcc
    10981 tggccacctc ggcgtcatcg tctcggtcgc gacatcaggg gcacgcgagg cggcgcatta
    11041 ccggcagcga ttggcaaaat tcgccgactt gtcccatgtc accgtggagg tccagcactg
    11101 tcattcgaca aatggctgac gtcgcacgcg atccgacttg gccgtcgtcg caacctgcaa
    11161 gatttagccc cggcggtggc agcgtgatca cgacggccat cgaaaatgcg cgacgttggg
    11221 gagccgttgg tcagccgcgc aggatttcac ataacggtct ttgaactgtc ttatcaggaa
    11281 cgcgatcgat ggacggctcg gagcggctgg ctctgggctg accggcgaaa gcgcccgtaa
    11341 gtcgagtggg attttcaaac gtgaacgcat cgattgatca ggccgccatc tcgctcggta
    11401 cggccaccct catgggcgca tgcatcggat tcgaacgcca atggcgtcaa cggatggccg
    11461 gcttgcggac caatacgctg gttgcgatcg gcgcggccag ctttgtgatc ttcgaaggtt
    11521 tgtttcccga cgatggtagc ccgacacggg tggccgccca ggttgtctcc gggatcggct
    11581 ttctcggtgc gggaatcatc ttccgcgaag gacttcacgt caccggcctc aacaccgcgg
    11641 ccacgctgtg gtgctcggca gccgtggggc tgttggcggg aagcggccat ccgcggcatg
    11701 cggcattggc aacggctttc gtcgttctcg tcaatttgac attgcggccg ctggttcgga
    11761 tcatcaaccg gcaaccgatc tcgcaggccg aaaccgactt tcactaccgg gttcgggtcg
    11821 tctgccgaaa cccggaagag gcgcatatcc gcgccctgct cttgcagagc acaagcaacg
    11881 gccagctcag cctgcggcgg ctcgatagca ctgacctcga ggagagcggg cgcgtcgaag
    11941 tcaccgcgca gctgacgtct cagtcgaaga gcgacaccgt cctcgaacag gtcgtcggcc
    12001 gccttagtct cgagccgacc gtctcggctg cgagttggag tgtcggacca atcctggaat
    12061 aacaaatcgt caaagaatga tccgcgcatc gggggcgatg atacgcctgt gcatgggatt
    12121 gggagcagca cgggcatttt cttggcgact cgctcccgcg aatcgcggcc gatctactcc
    12181 cgacctgcca gcgcctagtc attccgtgtg ctgtgttggc gcacgtcact caacgagcaa
    12241 caagcgcctt ccgccatcgt cttgcaattg cgacctattc gcaactgctt gccaggcaat
    12301 cggaaccgtg tcatcattat tgcgaaaaag tgtcggtagc attcagcggc aaaaatgcgg
    12361 gtttgggtca attgggcatg ttatctcgga acaggcatcg taatgatgct tgccggggtc
    12421 ggccccacaa attccgcgga gtattcggta agccgaccaa ccaagagctt ggctccggta
    12481 ccttttttga attggaccgg attttatgtc ggcggacacc tgggctacgg ccgtggacat
    12541 gcaagcggca gcatgacgga cgcggttccg acaatttcga gcaattcctt cgggagcctc
    12601 tttgggggct tgcagcttgg ctacaaccat ctcctgacgt caggctttct tgtgggtgtt
    12661 gaaagcgacg tttcgtttgc aaacttcctg ggtgctgacg atatcgtgcg gtcaaggttc
    12721 acagcgcagg gaaaccttgc ggaaaaaatc gactatatcg gaaggttgcg cggccgcctc
    12781 ggctacgcct ctggcgattg gctgttctac ggaactggcg gctttgcctg gtcgcaagcc
    12841 cgtttcatcg agactcccgg tctaatcgcc gatgaagaca aggtccttcg gacacgccca
    12901 ggctgggttt tgggcgccgg cgcagaagtc gccattgcac cggactggag cgccaggctg
    12961 gagtacctgt acgataattt tggaagggtt gatgccacct tgccttcagg gaggcgctcg
    13021 gattccacct tcgatacgca tacgctgcga ctggggttga actggcactt ggggcgagcg
    13081 gcatccgaat ccccatcgat cgagaaccgc gcttacccgg tcattgcacc cgacaattgg
    13141 aacgtgcacg gacaatatac attcgtcgga cagggctatc cgagcttccg ctcccctttt
    13201 gaaggtccga acagcctgac cgggtccaag caagctcaaa acaccatgag cgcgacggca
    13261 tttgttggtt tgcgcctttg ggagggcgct gaattttaca tcaatccgga attgatgcag
    13321 gggtttggcc tcagcaacgt gcatggcttg gccggattct caaacggaga ggcccaaaag
    13381 tcgagtttcg ccttgccgcg tatgaatgtt gcgcgcattt tcatacgaca gacttttggg
    13441 cttggcggcg agcaagagac gatcagcgac ggtcccaacc aactcgctgg caagcaggac
    13501 atctcccgca ttacggtgac cgcaggaaaa ttcgccgtta ccgatgcctt cgacggaaat
    13561 gtttacgcca acgatccgcg aacgactttt ctcaactgga atatttacgg cggcggttcc
    13621 tatgactgga ccatggacaa gttgagctgg acatggggcg ctttcgtgga tttcaatcag
    13681 aaagactggg ccttccgcac cggctatttt ctgctgccag tggtgtccaa cagcaacaat
    13741 ttcgacacgc acattccgga ccgcggccag tatacggctg aactcgaact gcgatactcg
    13801 ctgttttcgc aacccggcaa attgcgcttc ttcggctggc tcaatcacgg cacgatgggc
    13861 ggttactccg acgcgcttgc actacctcca actagcccag attatcccga cataacgctg
    13921 acgcgccgca tcagaaccaa ctacggtcta gtcgccaata tcgagcaggc gatcagcccc
    13981 gacctcggca tgttctcccg cgcgacgtgg agcccgggtc gtaccgaaat tctcggatgg
    14041 actgattgca gcgaaagcct ttccttcggc accgtgctga acgggacaag ttggggaagg
    14101 cccgatgata aaattggtgt cgcgggtgtc gtcgaaggtc tctcggcgga gtcgcgtgct
    14161 tatttcgctg ctggcggtct tggcattcta attggtgatg gccaactcaa ctacagcaga
    14221 gaaaaaatca tcgaggcgta ctacgcctac agcctcaaca aatgggctac gctaaccttc
    14281 gactaccagt tcatcaacaa tcccggatac aatgccgacc gcggtccggc ttcggtattt
    14341 tcgggcagat ttcacgcgga gttctgacga accaaggcac acacgtccat tctcgatcgc
    14401 cctcttattt cggttcggaa atcggcgtcg ccagcacctt gtcgatgcga cgtccgtcga
    14461 gatcaacgat ttcgaagcgc caaccgagag catcgacgtg ctcaccgaca ttcgggatag
    14521 tgccgaactg ctggagcaga aaccccgcaa tggtctgata ttgccgcgag gccggcaacg
    14581 ggaccttgag caactgcgcg aactcgatcg ccggcatcca gcctgaaatg agataagatc
    14641 catcgtcgcg tttcaccgcg gccggttctg ccggcccttc ttccgtatgg aagctgccaa
    14701 cgatggcctc cagaatatcg gcgctggtca ctacgccttg gaaggtaccg tactcatcgt
    14761 gcaccaatcc catgtgcacg ggcgaatccc gcaggatcga aacgacgtcc cgcgcgtcga
    14821 gggactccgg aatgatcggc gcatcccgga ccagctttcg ggcatccgga gtttgaccgg
    14881 acagataggt atcgagcaag tccttggcct ggataattcc caccgctgcg tctcggtcgc
    14941 gctcgaagac cacgaaacga gaatgggcgc ggttagccaa tgttgctttg atcacttcgg
    15001 gggcatccgt cagatttatc atgctgactt catggcgcgg cgtcatgacg gcaccgacgg
    15061 gaaggtcgcc gaggcgcatg acacccgcga tcatttcctt ttctccgggc tctaaaacgc
    15121 cagcgttctc cgcttcgaca accagggttc gtatttcctc ttcgctcact ttctcttccg
    15181 tctcgccacg ttgaccgagc agccacagca aggctttccc agagcgatca aggagccaca
    15241 ccagcggcag ggaaatcttc gaaagcagca ccatatgggg cgcaacgcgg accgcgactg
    15301 cttccggatc gcgcagcgcg atctgcttcg gaacaagttc gccgacgatg agcgaggcat
    15361 aggtgatgat cgtcacgacc gcgccgaccc cgatcgcgtc ggccaagccc tgtgacaatc
    15421 cgaactcgac aagccaggcc gtcagacgca accctagcgt tgctccggaa aacgcgccgg
    15481 aaagcacgcc tatgagagta attccgattt ggacggtgga cagaaacttg cctggatcag
    15541 acgcgagagc aagcgcgcgc cttgaacctt tgacgccctt ttcgaccaaa ccggccaggc
    15601 gggccggacg tgaagaaacg attgcgagct ccgacatgga aaggaggcca ttcacaatga
    15661 tcaggatcgt tacgatcccg agttccaccg ctaacatacg tcccataccg ttctgagggg
    15721 aattgtctgg ctgacagtac ggccgcgccg aagcgattaa caacggtcag aagtgtcgca
    15781 aggagaggcc gagcagcggt tgagggggtc atcttttttt ggtttcgaat ggttcattcc
    15841 gtgtgctgtg ttgcacgcac gtcactcgac gatgccattc aacagcatct tttgtgttca
    15901 ttcttgcaat tgcaaatgag ttgcaatagg gctgagacaa tggcattcgg aaaaaaatac
    15961 agggaatgaa ccgtgcctcc gcttgcgctc gcttgttgac cgcgggaatc ggctaatcca
    16021 gagggcgggg ccgacagctt cgcttgatgg atggcagcgc gccggcatga tcgcagggga
    16081 cgaaagagac cgccggaatg cccgcgctgg aaatatccgc tccatgatcc gcagcagccg
    16141 atcctgggtg tgcatattcg caggtgtggg cttgagcctg ctcgtcgtcg acggttccgc
    16201 gattgcagcc gacatgcctg ttaaagctcc ctacattcgg tcggtgttcg attggaccgg
    16261 gctctatgtc ggcgcccatg ccggcttcgg ccgcggctcc tcaaccgccg tgcttgcgga
    16321 tcccgcgacc agcacgacca gcaactcctt tggcggcatg atcggcggcg tgcaggccgg
    16381 ctacaattac caactgccga ccgggatgct gctcgggttg gaagccgaca tctcctttcc
    16441 aaattatctc acctcgaact cggtcgtctc ctcgttggca acgatccggt ccgacatcac
    16501 cgagcagtgg gactatgtgg gaaccgcgcg cggccgcgtc ggttatgtcg ccggtccgtg
    16561 gctggtctac gccaccggcg ggctcgcatg ggccggcgag cgctttctca acacgcccgc
    16621 catcggcagc gatgaaaaga cgctgcacac ccggctcggc tgggccgccg ggggtggggt
    16681 ggaatatgcc ttcgcgccgc actggagtgc ccgactggaa tatctctaca gccagttcgg
    16741 aaaggccgac atcgccttcc catctgcgac gcagtacacg tccagcctgg atttccagtc
    16801 gctgcggatt ggcctcaacc gtaagatcga ctggccggga tcgggcaact tctcaccggc
    16861 atcgctgacc gatcccgaat ccgatcgctg ggaaatccac ggccaatcga cctatttgcc
    16921 gcaaggctac ccggcattcc gcgcgcttta taccggtccg aacagcctga ccccggcgcc
    16981 gcaagtccag gcaacctgga gcaacagcct gtacctgaac gctcggctct gggaaggcgg
    17041 cgaggtctac tacaatcccg aactgctgca gggatttgga ttgagcgata cggttggagt
    17101 tgccggcttt cccagcggcg aatcgcaaaa atcaaacttc ccctacccgc actacaatac
    17161 gtcgcggctg ttcctgcgcc agaccttcgg ctttggcggt gaacaggaag aatggaccag
    17221 cggaccgagc caactcgccg gcaaggtgga cgtctctcgg ctgacgattc tggtcggcaa
    17281 attggctgtc accgatatct tcgacggcaa cgcctacgcc aaggacaccc gcaaggactt
    17341 catgaactgg tcgatgtggg cgccgggagc gttcgattat tcggcggaca agctcggcct
    17401 gacctatggc gccaccgcgg agttcaatca gaaacaatgg gcgttacgat caggctattt
    17461 cctggtggac gcggtatcga attccaacaa tttcgatacc aagctttttc agcgcggcga
    17521 atacgtggtt gagctcgaaa cacgctattc gctgttcaac cagccgggaa agctgcggac
    17581 catcgcatgg ctcaacagcg tcttttccgg aagttatcgc gagacactgg ataatccggc
    17641 attaaatctc gacattgcgc aaacccgcac cggccgcatc aaatatggct atgtcatcaa
    17701 tctcgaacag gccttgaccg atgaggtcgg gttgttcggc cgctggagct ggaacgacgg
    17761 caagaccgaa atcatggcgt tcaccgatat cgatgccagc ctctcgctcg gcgtctcaat
    17821 caagggcacc aaatggggca gacccgacga cgtgatcggg atcggcggcg ccatcaatgc
    17881 gctttcccgg gatcatcgcg acttcctcgc ggccggcggg ctcggccccc tgatcggcga
    17941 tggccagctc aactatcagc ccgagcgaat cctcgaaagc tattatgcgt acgcgctcaa
    18001 caagcagctc acgctgaccg gcgattatca gttgatcacc aaccccgcct acaatgcgga
    18061 tcgcgggccg gtccacgtct tttctggccg cctgcacgcc gagttctaga ctcgctatct
    18121 cggcttgcct gcgaacaatc catcaaaccg aaatgaatat ccgacaccga caacgtagct
    18181 cggcgcatta tggttgagtc cgaaagcgac gtggaaatcg acctgttgaa cagggctcag
    18241 atgatacaat ccacccgaat tcaggagctg gctcggacca gcattctcgg ggtaatcgcc
    18301 gacatattcc acaaacagac tggccctctc ggttaccttt ttttcgaccg caaaggtggc
    18361 ttccgtcatg cg
//
LOCUS       DATNOI010000463         4188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1625034,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000463 DATNOI010000000
VERSION     DATNOI010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625034"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="VN968_18485"
     CDS             complement(<1..1133)
                     /locus_tag="VN968_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXL31259.1"
                     /translation="MRTSLIVQGIAVILLGTAAQADDLKIALIHGKTGPLEAYAKQTE
                     TGLRMGLEYATKGTMTLDGRKIVVITKDDQSKPDLSKAALAEAYQDDKADIAIGTSSS
                     AAALADLPVAEENKKILIVEPAVADQITGEKWNRYIFRTGRNSSQDAISNAVAIGKKG
                     VTVATLAQDYAFGRDGVAAFKEALAKTGATLAAEEYVPTTTTDFTAAGQRLFDALKDK
                     PGRKIIWVIWAGAGNPLGKLQDMDPKRYGIELSTGGNILPALAAYKSLPGMEGATYYY
                     YDIPKNPVNDWLVAEHQKRFNAPPDFFTAGGFSAAMALVAAVTKAKSTDTEKLIAAME
                     GMEFDTPKGKMIFRKEDHQALQSMYHFKVKVDPNLAWAVNEPVR"
     gene            complement(1257..2219)
                     /locus_tag="VN968_18490"
     CDS             complement(1257..2219)
                     /locus_tag="VN968_18490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HXL31260.1"
                     /translation="MVKLIDEFRRGWQGTSEPSPLFSLVFAASCLTLATAARWGLSLI
                     RPDVFFIPYFPAVFFVTAFSGYRLGIAAAIFGGALGVTVNFGEAPTDFAKLALLVIYL
                     IVCGLTIWGVEHYRSVAAQQRQISKRLMQEERYRKLVVEEFQHRLKNKLSTIHAVIHL
                     VLQDQPQIWAGIDQRLRALSATDDLIARVDGTGCDIKDLLISELGPYGHVRFTLIGNP
                     LFLPAKLAVSLALIFHELATNAGKYGAFSSPRGLLHVSWSVSDDRLNITWDETGGPPV
                     ETIGAAGFGTRLLKSALTAFDGKTEITFLRTGLHCTMQCRVPPE"
     gene            2373..3215
                     /locus_tag="VN968_18495"
     CDS             2373..3215
                     /locus_tag="VN968_18495"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXL31261.1"
                     /translation="MGRLDGKVAVITGATSGIGLRTAEIFVAEGARIVVAGRRAPEGE
                     ALAKKLGAVCVFRQTDVTVEEQMRALIGEAVEKFGRIDCLFNNAGGPAQTGGIEGLEV
                     ARFDAAMATLVRSVMLGMKHAAPHMRRQGSGSIINNGSIAGRLAGYSTSVVYGAAKAA
                     VIHLTKCVAMELGEVGIRVNSISPGVIATGIFGKALGLSVEAAEKTPELMHGVYKSAQ
                     PIPRAGLPEDIAHAAVFLASDESSFVNGHDLVVDGAITGGRNWTQQQQGYVALRKAFD
                     QGKG"
     gene            complement(3226..>4188)
                     /locus_tag="VN968_18500"
     CDS             complement(3226..>4188)
                     /locus_tag="VN968_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXL31262.1"
                     /translation="GIAARVAQWKARPGPMLFYPAMKQLTLDLAATSFLGAGIGPEVD
                     EITRAFVDMVAASISVIRRPLPFTKMARGVRGRERIVAYFSEQIPIRRARGGEDLFSQ
                     LCQATHEDGALLSTQDIVDHMSFLMMAAHDTLTSSLTSFVGALAAHPQWQTKLREEVG
                     ALGIAADQPTNFDNLEAMPLTEMAFKEAMRLKPPVPSIPRRAIRDLVFKGYAIPAGTM
                     VGVNPLFTHHMPEIWPDPDAFDPSRFTEEAQRNRHRFAWVPFGGGAHMCLGLHFAYMQ
                     AKCFARHFLQNLSVSLEPGYTPDWQMWPIPKPRDGLRVTLRPVA"
BASE COUNT          787 a         1293 c         1341 g          767 t
ORIGIN      
        1 cgcaccggtt cgttcaccgc ccaggcgagg ttcggatcga ccttgacctt gaagtgatac
       61 atgctttgca gcgcctgatg gtcctccttg cggaagatca tcttgccctt cggcgtatcg
      121 aactccatgc cttccatcgc agcgatcagc ttttcggtgt cggtggattt tgccttggtg
      181 acggcggcga ccaacgccat cgcggcggaa aacccgccgg cagtgaagaa atccggcggc
      241 gcgttgaagc gcttctggtg ttcggcgacg agccagtcgt tcaccgggtt cttcggaatg
      301 tcgtagtagt aataggtcgc gccttccatg cccggcaggc tcttataggc ggcgagcgcc
      361 ggcaggatgt tgccgccggt cgagagttcg atgccataac gcttcgggtc catgtcctgc
      421 agcttgccta gcgggttgcc ggcaccggcc cagatcaccc agatgatctt gcgtcccggc
      481 ttgtccttga gcgcgtcgaa caggcgctgg ccggccgcgg tgaagtccgt ggtggtggtc
      541 ggcacatatt cctccgccgc aagcgtcgcg ccggtctttg ccagcgcctc cttgaaggcg
      601 gcgacgccgt cgcggccaaa ggcatagtcc tgggccagcg tggcgacggt gacgcctttc
      661 ttgccgatcg ccaccgcgtt ggagatcgcg tcctgcgagg aattacggcc ggtgcggaag
      721 atgtacctgt tccacttctc gccggtaatc tggtccgcga ccgccggctc gacgatcagg
      781 atcttcttgt tttcctcggc caccggcagg tcagccaggg ctgcggccga cgatgacgtc
      841 ccgatggcga tatcggcctt gtcgtcctgg taggcttcgg cgagcgccgc cttggataaa
      901 tccggcttgc tctgatcgtc cttggtgatg accacgatct tgcgcccgtc cagcgtcatg
      961 gtgcccttgg tggcatattc cagtcccatg cgcaggccgg tctcggtctg tttcgcatag
     1021 gcttcgagcg gtccggtctt gccgtggatc agggcgatct tcagatcgtc ggcctgagcg
     1081 gccgtcccca gcaagatgac ggcaattccc tgaacgatta aagacgttcg cacagctatt
     1141 ctccctcaag tttctgatct gtacgtcact gatagagtga gcgctcgccc cgggtcaagg
     1201 cagcattgtc gcgtaggcaa cggcgcgtgg ggtctatccg gggcgctcga agcacttcac
     1261 tccggcggta cgcggcattg catcgtgcaa tgaagccctg tcctcaaaaa ggtgatctcg
     1321 gtcttgccat cgaacgccgt cagcgccgac ttcaggagcc tggtgccaaa gccggccgcc
     1381 ccgatggttt cgaccggagg gcccccggtc tcgtcccagg tgatattgag gcgatcgtcc
     1441 gacaccgacc acgacacatg caacagcccg cgcggcgagg aaaacgcgcc gtatttgccc
     1501 gcattggtgg cgagttcgtg aaatatcaat gccaggctca ccgccagttt ggccgggaga
     1561 aacagcggat ttccgatcag ggtaaatcga acatggccgt aaggtccgag ttcggaaatc
     1621 agcagatcct tgatatcgca accggtgccg tcgacccgtg cgatcagatc gtcggtcgcc
     1681 gacagcgccc gcaatcgctg atcgatgccg gcccagattt gcggttgatc ctgcagcacc
     1741 agatggatga cggcgtgaat cgtcgacaac ttgttcttca gccggtgctg gaattcctcg
     1801 acgacgagct tgcgataccg ctcttcctgc atcaggcgtt ttgagatttg ccgttgctgc
     1861 gcggcgaccg atcgatagtg ctcgacgccc caaatcgtca ggccgcacac gatcaggtag
     1921 atcaccagaa gcgccagttt tgcaaaatcc gttggggcct cgccaaaatt gacggtcacg
     1981 ccaagcgcgc cgccaaaaat cgcggccgcg atcccgagcc ggtagccact gaacgcagtg
     2041 acaaagaaca cggcgggaaa atacgggatg aagaacacgt ccggccggat cagcgataga
     2101 ccccaccgcg ccgcggtcgc cagggtaagg caggaggctg caaatacgag gctgaaaagc
     2161 ggcgacggtt cggatgttcc ctgccagccg cgccggaact cgtcgatcaa tttcaccatg
     2221 ggttacaaca caagctgact ggccaatccg gaataacgca agccggagga aaaagacgcg
     2281 gtctgcggcc tcgcccaggc aagcatagct tgcgttgtcc cgtcagtccg gaaatatact
     2341 cgatgccagg atcgcgaaac ggggagaacg acatgggacg gctggacggc aaggtcgcgg
     2401 ttatcaccgg cgcgacgagc gggatcgggc tacgcaccgc ggagattttc gtggccgaag
     2461 gcgccaggat cgtcgtcgct gggcgccgcg cgccggaggg cgaggcgctg gcgaaaaagc
     2521 tcggcgccgt ctgtgtgttc cgccagaccg atgtcaccgt cgaggagcag atgcgcgcgc
     2581 tgattggcga ggcggtcgag aaattcggcc gcatcgattg cctgttcaac aatgccggcg
     2641 gcccggcgca gaccggcggc atcgaaggcc tcgaggtagc gcgcttcgac gctgcgatgg
     2701 caaccctggt gcgcagcgtg atgctcggca tgaagcacgc agcgccccat atgcgccgcc
     2761 agggttcggg cagcatcatc aacaatggca gcattgccgg acggctggcg ggctattcca
     2821 cctccgtggt ctatggcgcg gccaaggccg ccgtcattca cctcaccaaa tgcgtggcaa
     2881 tggagctcgg cgaagtcggc atccgcgtca actcgatttc gccgggcgtg attgccaccg
     2941 gcattttcgg caaggcgctc ggcctttcgg tcgaggcggc ggagaagacg ccggagctga
     3001 tgcacggggt ctacaaatcg gcgcagccga tcccgcgcgc cgggctgccg gaagatatcg
     3061 ctcacgccgc ggtgtttctc gccagcgacg aatccagctt cgtcaacgga cacgatctgg
     3121 tggtcgatgg cgccatcacc ggcggccgca actggacaca gcagcagcag ggctatgtcg
     3181 cgctgcgcaa ggcgttcgat cagggcaaag ggtaacccct cgcgcttacg caaccggcct
     3241 cagcgtcacg cgcaatccgt cccgcggctt cgggatcggc cacatctgcc aatccggcgt
     3301 gtagcccggc tccagcgaga ccgagagatt ttgcaggaaa tgccgggcga agcatttcgc
     3361 ctgcatatag gcaaagtgca ggccgaggca catatgcgcg ccgccgccga acggcaccca
     3421 ggcgaagcga tggcggttgc gctgcgcttc ctcggtgaag cgcgagggat cgaacgcatc
     3481 gggatcgggc cagatctcgg gcatgtggtg ggtgaacaac ggattgacgc cgaccatagt
     3541 gccggccggg attgcgtagc ccttgaatac gagatcgcgg atagcgcgac gcgggatcga
     3601 gggtaccggc ggcttcaatc gcatcgcttc cttgaacgcc atctcggtga gcggcatggc
     3661 ctcgagattg tcgaaattcg tcggttgatc ggcggcgata ccgagcgcgc caacttcctc
     3721 gcgcagtttg gtctgccact gcggatgggc ggcaagcgcc cccacaaacg acgtcagcga
     3781 cgacgtcagc gtgtcgtgcg cggccatcat cagaaaactc atatggtcga cgatatcctg
     3841 ggtcgacagc agcgcgccgt cctcgtgggt ggcctggcac agctgcgaga acagatcctc
     3901 gccgcctctg gcgcggcgga tcggaatctg ctccgagaaa tacgccacga tgcgttcgcg
     3961 gccccgcacg ccgcgcgcca tcttggtgaa gggcagcggc ctgcgaatga ccgagatcga
     4021 ggcggccacc atgtcgacaa aggcgcgcgt gatctcatcg acctcggggc cgatgccggc
     4081 gccgagaaaa gaggttgcgg cgagatcgag ggtaagctgt ttcatcgcgg ggtaaaacag
     4141 catcggcccc ggtcgcgcct tccattgcgc gacccgcgct gcgatccc
//
LOCUS       DATNOI010000464         4446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_552199,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000464 DATNOI010000000
VERSION     DATNOI010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552199"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..2044)
                     /gene="malQ"
                     /locus_tag="VN968_18505"
     CDS             complement(86..2044)
                     /gene="malQ"
                     /locus_tag="VN968_18505"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HXL31263.1"
                     /translation="MDLYNKAKNLGIQTEFLDGQGHRHVTDAEALKIILDALPVRKPY
                     RFLEQAVVIRSGRPARTELGHAATFPLRWKIVAGLKVIAVGETHDRVIGWPADLPVGS
                     YRVHLTDASSFTEEAPLIVAPPRAFDGDFDRCWLLAVQLYGVRSARNWGIGDFTDLAG
                     LIELAGHLGADGVGLNPLHALFDDRPADCSPYSPNSRLFLNSLYIDVEKLPEFQRGVF
                     ATSGKDIARLRASDTVDYIGVAELKWRALRLAFETFKADANNARRQDFEKFRAERAPL
                     LSRFACFEVLRHKFNKPWWEWPEEWRKPDDVKFARLRAGTDATEIEFVEFVQWTADRQ
                     LQSCRDLAVRRGMKVGLYLDVAVGVQADGFDAWNEQVAISRHLAVGAPPDSLNTSGQN
                     WGLAGFNAAGLEIKSFEPYRDMLRASMRYAGAIRLDHVLGLKRLYLVPHGFAADNGVY
                     VQMPFEALLAATALESVAYRCVVIGEDLGTVPEGFREQIADWGIWSYQVMMFERDDQG
                     KFRDIDHYAPNALVTFNTHDLSTYAGWRSFSDLALKRSLGIDPGESDDARWHALAMLT
                     DVLRHHGIDRHDLNAVVGFLARTKSRLLAISLEDLLGVIDQPNIPGTVNEHPNWRQRL
                     PLAIDEIASAIDIPALKTATAERTRMAT"
     gene            2245..4200
                     /locus_tag="VN968_18510"
     CDS             2245..4200
                     /locus_tag="VN968_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HXL31264.1"
                     /translation="MNKTTQTVESAAAVGAFHIEDVYPLIDGGRFPVKRIVGERVEVW
                     ADVYRDGHDVVAAALLWRRERDREWRREPMTHHGNDRWVGSFVPDQPGRHVYAIEAWT
                     DEFATWRHGFELKQKAGADLTLDAIEGAGMLTKAQAGGQAAATVILKQCEDFLQTGDA
                     APLLTTELKDAMAESQLRPDLTRSQLFPLVVDRPRARSGAWYEMVPRSQGEIPGQHGT
                     FKDSIARLPDIAAMGFDVVYLTPIHPIGRTNRKGRNNAVTSAEGDPGSPYAIGAAEGG
                     HDAVHPELGTLEDFREFVAACNRLEMEVALDFAIQCSPDHPWLKQHPQWFRRRPDGSM
                     RYAENPPKKYEDIVNPDFSSEDAGALWNALRDVVLFWIEQGVKIFRVDNPHTKPFRFW
                     EWLVREVQLRHPDVIFLAEAFTRPKLMKGLAKLGFTQSYTYFTWRTQKWELEQYLTEL
                     TAYPERDYYRPNFFVNTPDILPWHLQSGEPWMFKSRVALAATLSASYGIYNGFELLEH
                     DPIPSREEYLDSEKYEIKVRDWDKPGNIKPYIRDLNRARRANPALQQTSQLRFLPIDD
                     GNVIGFVKESVDQTNTVAVAIALSREVHEFWLPLGDVQLGTGSDRRHVAAVENLITGE
                     RYPVEWGGIRLRIDPVRDPAFLFRCLA"
     gene            4209..>4446
                     /locus_tag="VN968_18515"
     CDS             4209..>4446
                     /locus_tag="VN968_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXL31265.1"
                     /translation="MNLLPSIDATELPVTALDRDELWYKDAIVYQLHVKAFADSNNDG
                     IGDFAGLTEKLDYLQDLGVTALWLLPFYPSPGRDD"
BASE COUNT          882 a         1376 c         1342 g          846 t
ORIGIN      
        1 ccgcggtctc gcaatccccg atcagtccat agtcttcgat cttttggggc aaattcctat
       61 tccccgctga agatgattca acgcctcagg tcgccatccg ggtccgctcc gcggtggccg
      121 ttttgagcgc ggggatgtcg atcgccgagg caatctcgtc gatcgcaagc ggcagccgct
      181 gccgccagtt cgggtgctcg ttcacggtgc cgggaatgtt gggctgatcg atcacgccaa
      241 gcaaatcttc cagcgagatt gcaagcaggc gggatttggt acgggccaaa aagcccacca
      301 cggcattgag gtcatgccgg tcgatgccgt gatgacgcaa gacgtcagtc agcatggcga
      361 gggcatgcca acgcgcgtcg tcgctttcgc cagggtcgat cccgagcgac cgtttcagcg
      421 cgagatcgct gaacgagcgc cagccggcat aggtggagag atcgtgggtg ttgaaggtaa
      481 ccaaggcatt tggcgcataa tggtcgatgt cgcgaaactt gccctgatcg tcgcgctcga
      541 acatcatcac ctggtatgac cagatgcccc aatcggcgat ttgctcgcgg aagccctccg
      601 gcacggtgcc gagatcctcg ccgatcacca cgcaacgata cgccacgctt tccagcgcgg
      661 tcgccgccag cagcgcctcg aacggcattt gcacatagac gccgttgtcg gcggcaaaac
      721 cgtgcggcac gagatagagc cgcttcaggc cgagaacatg atcgagccgg atcgcgcccg
      781 cgtaacgcat cgaagcccga agcatgtccc gatacggttc gaaggacttg atttccagac
      841 cggcggcgtt gaacccggcg aggccccagt tttggcctga cgtattcagc gagtccggcg
      901 gcgcgccgac cgcgagatgc cgggaaatcg cgacctgctc gttccaggca tcgaatccgt
      961 ccgcttgcac gccgacggcg acatcgaggt aaagcccgac tttcatgcca cgccgtaccg
     1021 cgagatcccg gcaggactgc aactggcggt cggcggtcca ttgcacgaat tccacgaatt
     1081 cgatctcggt tgcatccgtg cccgcacgca gtctggcgaa tttgacatcg tcgggttttc
     1141 gccattcctc cggccattcc caccacggct tgttgaattt gtgccgcagc acttcgaagc
     1201 aggcgaaccg cgacagcagc ggcgcgcgct cggcgcgaaa tttctcgaaa tcctgccggc
     1261 gtgcattgtt cgcatcggcc ttaaaagttt cgaacgccag gcgcagcgcg cgccatttca
     1321 gctcggcgac gccgatatag tcgacggtat cgctcgctct cagccgggcg atgtctttac
     1381 cgctcgtggc gaaaacgccg cgctgaaatt ccgggagttt ttcgacgtcg atatagagtg
     1441 aattcaggaa cagccggctg ttcggtgaat aggggctgca atcggcgggg cggtcgtcga
     1501 acaatgcgtg cagcggattg aggccaacac cgtccgcccc caaatggcct gcgagttcga
     1561 tcaggcctgc gaggtcggtg aagtccccga tgccccagtt gcgcgctgag cggacgccat
     1621 agagctggac tgccaatagc cagcagcggt cgaaatcgcc gtcgaaagcc cttggcggag
     1681 ccacgatcaa cggcgcttct tcagtgaatg acgaggcatc ggtcaaatgc acccggtacg
     1741 aaccgacagg caaatccgcg ggccagccga tgacgcgatc atgggtttcg cccactgcga
     1801 taaccttaag acccgcaacg attttccagc gcaacggaaa cgtggcagcg tggcccagtt
     1861 cggtccgcgc gggtcggccg gagcggatca ccaccgcctg ctcgagaaat cggtagggtt
     1921 tccgtaccgg aagggcatcg agaatgatct tcagtgcctc agcgtcggtc acatggcggt
     1981 gaccctggcc gtccaggaat tcggtctgga ttccgaggtt tttagctttg ttgtaaaggt
     2041 ccattcggca cgctgtttgc acgcaacggt tagggacgtc gcgaaattct ctagatgcgg
     2101 taagaggata accgtgttta actctctggt ggcgccggcg ttccccggga accaatgccc
     2161 ggccagcggc tttctatatg tgttcgcagc gtctcctttc ccgcaatcga aacgggacgt
     2221 catatccatg tcaatggcat caccgtgaac aaaacaactc aaactgtcga gagcgccgcg
     2281 gccgtcggcg cttttcatat tgaggatgtc tatccgctca tcgatggcgg ccgctttccc
     2341 gtgaagcgga tcgtcggcga gcgcgtcgaa gtctgggcgg atgtctatcg tgacgggcat
     2401 gatgttgtgg cggcggcgct gctgtggcgg cgcgagcgtg accgggaatg gcgccgcgag
     2461 ccgatgaccc atcacggcaa cgaccgctgg gtcggatcgt tcgtgccgga ccagcccgga
     2521 cgccatgtct atgcgatcga ggcctggacc gacgaattcg ccacctggcg gcacggcttc
     2581 gaactcaagc aaaaggccgg cgctgatctt accctcgatg cgatcgaggg cgcgggcatg
     2641 ctcaccaagg cacaggccgg cggacaggcc gcggccactg tgatcctcaa gcaatgcgag
     2701 gactttcttc agaccggcga tgccgcgcct ctgctcacca ccgaattgaa agacgccatg
     2761 gcggagagcc agttgcggcc cgacctgacg cgatcgcaat tatttccatt ggtggtggac
     2821 cggccgcggg cgcgctccgg cgcctggtac gagatggtgc cgcgcagcca gggagagatt
     2881 ccgggccagc acggcacatt caaggacagc atcgcgcgat tgcctgacat cgcggcgatg
     2941 ggttttgacg tggtctatct cacgccgatt cacccgatcg gccgcaccaa ccgcaagggc
     3001 cgcaacaacg cggtgacttc agcggaaggc gatcccggaa gcccgtacgc gatcggcgcc
     3061 gccgagggcg gccacgatgc ggtgcatccc gaactgggca cgttggagga tttccgtgag
     3121 ttcgttgcgg cgtgcaaccg gcttgagatg gaagtcgcgc tcgacttcgc gattcaatgc
     3181 tcgccggatc acccctggct caagcagcat ccgcaatggt tcaggcgtcg gccggacggc
     3241 tcgatgcgct atgcggaaaa cccgcccaag aaatatgagg acatcgtcaa tcccgacttt
     3301 tcgtcggagg acgccggcgc gctgtggaac gcgttgcgcg atgtagtgct gttctggatc
     3361 gaacagggcg tcaagatctt ccgggtcgac aacccgcaca ccaaaccctt cagattctgg
     3421 gaatggctgg tccgggaagt tcagctccgc catcccgatg tcattttcct cgccgaggcg
     3481 tttacgcggc ccaagctgat gaagggcctc gccaagcttg gcttcacgca atcctatacc
     3541 tactttacct ggcggacgca gaagtgggag ctggagcagt atctgaccga actcaccgcc
     3601 tatccggaac gcgattacta tcgcccgaat ttcttcgtca atacgccgga tatcctgccc
     3661 tggcacctgc aaagcggcga gccctggatg ttcaaatccc gcgtcgcgct tgcggccacg
     3721 ctgtcagcca gctacggcat ctacaacgga ttcgagcttt tggaacatga tccaattcca
     3781 agtcgcgaag aatatctcga ttccgaaaaa tacgagatca aggtgcggga ctgggacaaa
     3841 ccgggcaata tcaagcccta catccgcgat ctcaaccgcg cccgccgggc aaacccggcg
     3901 ctgcagcaga caagtcaatt gcgcttcctt ccgatcgatg acggcaacgt catcggcttc
     3961 gtgaaggaat cggtcgacca aaccaacacc gtcgccgtcg caatcgcgct gtcgcgcgag
     4021 gtgcacgaat tctggcttcc gcttggcgac gtccagctcg ggacgggcag cgatcgccgc
     4081 cacgtcgccg cggttgagaa cctgatcacc ggcgagcgct atccggtcga gtggggcggc
     4141 attcgactgc ggatcgatcc agtgcgcgat cccgccttcc tgtttcgctg cctggcgtga
     4201 gcggctccat gaaccttttg ccatccatag atgcaaccga actgccggtc acggcgctcg
     4261 atcgcgacga gctttggtac aaggatgcca tcgtctatca gctccacgtc aaggctttcg
     4321 ccgacagcaa caatgacggc atcggcgatt tcgcaggctt gaccgaaaag ctcgactatc
     4381 tgcaggacct cggcgtcacc gcgctatggc tattgccgtt ctatccctcg cccggccgcg
     4441 atgacg
//
LOCUS       DATNOI010000465         8834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_552365,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000465 DATNOI010000000
VERSION     DATNOI010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8834
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552365"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1854
                     /locus_tag="VN968_18520"
     CDS             109..1854
                     /locus_tag="VN968_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HXL31266.1"
                     /translation="MAAVSSARRSELGDALRACRSAFIGVGIMSCMINLLYLTGSIFM
                     LEVYDRVLPSRSVPTLVGLAILAGGLYIAQGVLDLIRGRILIRIGTALDETLNARVFE
                     TVVRLPLLVGGRNEGLQPLRDLDNVRSFLSSMGPGAFFDLPWLPFYLAICFAFHVMIG
                     LTALVGAFILVTLTIVTEFMSRVPAREAMGLAARRNDLAATSRRNAEVLVAMGMSGRL
                     TKRWSEANEKYLAGNQRASDVSGGLGAIAKVMRMTLQSAVLAVGAYLVIHQEATAGII
                     IAGSILSARALAPVDLAIAHWKGFVAARQSWHRLNRLLEQLPARTVPTLLQDPSKRLS
                     VEAVSIVPPGDQKVIVQDVTFALEAGSGLGVIGPSGSGKSSLVRALVGVWQPLRGKVR
                     LDGAALDQWSSDVLGRHIGYLPQDVELFAGSVAQNICRFDTDAASDAIIAAAKEAGVH
                     GMIIKMRDGYDTQIGEQGTALSAGQAQRVALARALYGDPFLIVLDEPNSNLDTEGDEA
                     LTRAVRAARERGAIVVVVAHRPIGIEAVDQLLVLKDGRMQAFGPKEQVLGQVLQRVPP
                     PSPIKIVSDSGVAKS"
     gene            1899..3224
                     /locus_tag="VN968_18525"
     CDS             1899..3224
                     /locus_tag="VN968_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HXL31267.1"
                     /translation="MIGNLTRPGNDTGARPSIRLHLIIGLAVVAFLAIGLGGWATTAE
                     ISGALIAPGSVVVESNVKKVQHPTGGVVGEVLARDGDLVKAGDVVVRLDDTVTKASLA
                     IVTKNLDGLLARGARLEAEQRGVDKIAFPKALLERAGDPDVKNIIASETKLFEVRTFG
                     RVGQKAQLRERVTQLNEEIAGLMAQEKAKDQEIVLVEKELTGVQQLYDKHLVQMSRLT
                     TLERDAARLNGERAQFIAARAQAKGKITETELQIIQVDKDLVSDVSKDLRETNDKIGE
                     FVERKVTAEDQLRRTDIRAPQDGMVLQSTVHTVGGVITAGDAVMMIVPQSDALSVEAK
                     VNPQDIDKLQIGQRTLLRLSAFNQRTTPELNGVVTRVSPDVTTDQRTGQSYYTIRVSM
                     PSEEIKRLGDAKLIPGMPVEAFVQTGDRTMLSYLIKPLSDQLMRAFREK"
     gene            complement(4503..4985)
                     /locus_tag="VN968_18530"
     CDS             complement(4503..4985)
                     /locus_tag="VN968_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HXL31268.1"
                     /translation="MQDLVAIAEKIAAKLVERRQTIAVAESSTGGLISAALLAVPGAS
                     AYFLGGAVVYTRDARRILMDIPDDAMKGIRSASEAYAKLLASQIRTRFATDWGLSETG
                     ATGPTGNRYGDAAGHSCIGVAGLEQYAMTIETGSADRVANMQVFAATALNFLLQTLSH
                     "
     gene            5228..6382
                     /locus_tag="VN968_18535"
     CDS             5228..6382
                     /locus_tag="VN968_18535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL31269.1"
                     /translation="MTSPIAGGVDCDLHPTVPHLTSLLPYLNDYWRDQVTTRGMTDLV
                     SQSYPTHSPISSRPDWRPAQGKPGSNPADMKAQALDRFGVSYAICNPLYGVQMVFSED
                     MADAFCRALNDWLVKEWLDRDARLRGSIVIPVQSIEKSVAEIERCAVDKRFVQVLMLV
                     MGDMPLGKRAYWPIYAAAERLGLAVGIHAGSNYHNPPTSVGWGSYHIEDYVAQAQAFQ
                     TQLTSLIVEGVFAKYPKLKMVMLESGFTWLPSYLWRLHKFWRGVRMETPWVDRAPLEI
                     VRSNIRFSLQPIDAPPDPATLNRLFDHMQSDELLLFSTDYPHWQFDGDAVLPEGMSPD
                     LVRKIMIDNPLATYGRLKEPAHGHDPEKHALGPRPDGWNPVFGKDHAQTR"
     gene            6440..7549
                     /locus_tag="VN968_18540"
     CDS             6440..7549
                     /locus_tag="VN968_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL31270.1"
                     /translation="MNVQFRSGAEPASSVGVKTAIADCDIHPARATATELYPYLAKRW
                     HAHLETYGKHPYQGMMEGPPYPKAQPNASRRDAWPPDGGPQGSSLSFMQQQHLDPNNV
                     ALGVLCPLNTGQGIRNQDLSAALATAINDWQIDKWTSQDPRLKGSIVVANEDGPAAAA
                     EIRKRAGDKNFVQVLLLSRNVEPLGQRRYWPIYEAAEEAGLPIGVHAFGFGGNPITAS
                     GWPSFYIEEMVGHSQCQQTALASIVLEGVFERHPKLKMVMIEAGFGWAPSLSWRLDKS
                     WQRLKSEVPHVKRPPSEYIRDHIFWTTQPMEDPERRDHLFDVIDWVGWDKLLFATDYP
                     HWDYDEPSRVLPAGVSDANREAFYLGNARKLYGLS"
     gene            7549..7962
                     /locus_tag="VN968_18545"
     CDS             7549..7962
                     /locus_tag="VN968_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HXL31271.1"
                     /translation="MVRHVIAPVDELPPGTRKFLTIDARPIAVFNIKGEFFGLLNRCP
                     HQGAALCEGPLIGLAQSSDPGEIEYTRLGEIIRCPWHGWEFDIRTGQSYCDPKRFRAK
                     TYPANVEPGSSVVKGPYVAETIAVSVESDYVVVDL"
     gene            complement(8071..>8834)
                     /gene="tcuB"
                     /locus_tag="VN968_18550"
     CDS             complement(8071..>8834)
                     /gene="tcuB"
                     /locus_tag="VN968_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HXL31272.1"
                     /translation="FIFGFAALNDRQVLLGIHTGPGAFYALMPHNAMAALFGAAFLYA
                     IAALAMGVRAFWRDIGEPIRMLVDPPSLWQAMMDAGQLRYLDGGGVGCVNEDERPSDR
                     RKIFHHFTFYGFLLCFAATCVATLYHYLIAREAPYPWWDLPVVLGTLGGIGLLIGPAG
                     LLKEKWKRDPALGDEKRSGMDVAFIAMLFLTSLTGIALLLLRDTAAMGPLLALHLGVV
                     FSLFITMPYGKFVHGIYRYVALVRYARERRMLGHV"
BASE COUNT         1716 a         2736 c         2820 g         1562 t
ORIGIN      
        1 cggggataag gctaccgccg gcgtttacgt taatgtgact taaatggacg atagccggaa
       61 agatcggtaa gactggcggt tccaccgctt gcaaagagat caggatagat ggcagccgtt
      121 tccagtgccc ggcgttccga gcttggtgat gcgctgcgcg cctgccgcag cgccttcatc
      181 ggcgtcggca tcatgagctg catgatcaac ctgctctatc tgaccggctc gatcttcatg
      241 ctggaggtct acgaccgggt gctgccaagc cgcagcgtgc cgaccctggt tggtctcgcg
      301 atcctggccg gcggattgta catcgcgcaa ggcgtgctcg acctgatccg cggacgcatc
      361 ctgatccgga tcggaaccgc gctcgacgag accctcaacg cccgggtgtt cgaaaccgtg
      421 gtgcggctgc cgctgctggt gggcggacgc aacgagggcc tgcagccgct gcgggatctc
      481 gacaacgttc gctcgtttct aagcagcatg gggccgggcg cgttcttcga cctgccgtgg
      541 ctgccgttct acctcgcgat ctgcttcgcc ttccatgtca tgatcggcct caccgcactg
      601 gtcggcgcct tcatcctggt cacgctgacc atcgtcaccg aattcatgtc gcgcgtgccg
      661 gcgcgggagg cgatgggtct cgccgcgcgc cgcaacgacc tcgccgccac cagccggcgc
      721 aacgccgaag tgctggtggc gatgggcatg tccggccgcc tgaccaaacg ctggagcgag
      781 gccaacgaaa aatatctcgc cggcaatcag cgcgccagcg acgtctcggg cgggctcggc
      841 gcgatcgcca aggtcatgcg catgacgctg cagtcggcgg tgctcgccgt cggcgcctac
      901 ctcgtgattc atcaggaagc caccgccggc atcatcatcg cgggctcgat cctgagcgcg
      961 cgggcgctgg cgccggtcga tctcgcgatc gcgcattgga agggttttgt ggccgcccgc
     1021 cagagctggc accgcctcaa ccggctgctc gagcagctgc ccgcgcggac cgtgccgacg
     1081 ctgctgcagg atccgtcaaa gcgtctctcg gtcgaggcgg tcagcatcgt gccgccgggc
     1141 gatcagaagg tgatcgtcca ggacgtgacc ttcgcgctcg aggccggcag cggccttggc
     1201 gtgatcgggc ccagcggatc gggcaaatcg tcgctggtgc gggcgctggt cggcgtctgg
     1261 cagccgttgc gcggcaaagt gcgcctcgat ggcgcggcgc tcgatcagtg gtcgtccgat
     1321 gtgctcggtc gtcacatcgg ctatctgccg caggatgtcg aactgttcgc gggctccgtc
     1381 gcccagaaca tctgccgttt cgatacggac gcggcttccg acgccatcat cgccgccgcc
     1441 aaggaagccg gcgtccacgg catgatcatc aagatgcgcg acggctacga cacccagatc
     1501 ggcgagcagg gcaccgccct ttcggccggc caggcgcagc gggtagcgct ggcgcgggcg
     1561 ctgtatggcg atccgttcct gatcgtgctc gacgagccga actccaatct cgataccgaa
     1621 ggcgatgagg cgctgacgcg cgcggttcgc gccgcgcgcg agcgtggcgc catcgtggtg
     1681 gtggttgcgc atcggccgat cggaatcgag gcggtcgacc agcttctggt gctgaaagac
     1741 ggccgcatgc aggcattcgg ccccaaggaa caggtgctcg ggcaggtgct gcagcgggtg
     1801 ccgccgccgt cgccgatcaa gatcgtgtcc gattccggag ttgccaagtc atgatcgacg
     1861 gcaacaccat cgcgggcatc agggccatga ccggcaacat gattggcaac ctcaccaggc
     1921 cgggcaacga caccggcgcg cgcccctcga tccggctgca tctgatcatt ggccttgcgg
     1981 tggtcgcgtt cctcgccatc gggcttggcg gctgggccac gacggccgag atatccggcg
     2041 ccctgatcgc gccgggatcg gtcgtggtcg aatccaacgt caagaaggtg cagcacccga
     2101 ccggcggcgt ggtcggcgaa gtgctggcgc gcgatggcga tctggtcaag gccggcgacg
     2161 tggtggtgcg gctcgacgac actgtcacca aggcgagcct tgccatcgtc accaagaatc
     2221 tcgacgggct gttggcgcgt ggcgcgagac ttgaagccga gcaacgcggc gttgacaaga
     2281 tcgcatttcc caaagcgttg ctcgagcgcg ccggcgaccc agacgtcaag aacatcatcg
     2341 ccagcgagac gaaattgttc gaggtccgca ccttcgggcg ggtcgggcaa aaggcgcaat
     2401 tgcgcgagcg cgtcacacag ctgaacgagg aaatcgcggg cttaatggcg caggaaaaag
     2461 caaaagacca ggaaatcgtg cttgtggaaa aggagctgac cggggttcaa caactctacg
     2521 acaagcacct ggtgcagatg tcgcgcctga cgacattgga gcgcgacgcg gcccgcctca
     2581 acggcgagcg ggcgcagttc attgccgcga gggcgcaggc caagggcaag attaccgaaa
     2641 ccgaattgca gatcatccag gtcgacaagg atctggtcag cgacgtctcg aaggatttgc
     2701 gcgagaccaa cgacaagatc ggcgaattcg tcgagcgcaa ggtcaccgcc gaggaccagt
     2761 tgcggcggac cgacatccgc gccccgcagg acggcatggt gctgcagtcg acggttcaca
     2821 ccgtcggcgg ggtgatcacc gccggcgatg ccgtcatgat gatcgtgccg cagtccgatg
     2881 cgctgtcggt cgaggcaaaa gtcaatccgc aggatatcga caagctgcag atcggccaga
     2941 gaacgctgct gcggctgtcg gccttcaacc agcgcaccac gccggaactg aacggcgtcg
     3001 tcacccgggt ctcgcccgac gtcaccaccg accagcgcac cggacaaagc tactacacca
     3061 tccgcgtctc gatgccgtca gaagagatca agcgcctcgg cgacgccaag ctgatccccg
     3121 gcatgccggt cgaagccttc gtgcaaaccg gcgaccgcac catgctgtcg tatctgatca
     3181 agccgctgag cgatcaattg atgcgggcgt ttagggagaa gtgacggcta acctcgtctg
     3241 acaggctcgg caaatccatc caggccgtca ttccggggcg atgcgaagca tcgaacccgg
     3301 aatctcgaga ttccgggtct ggtgctgacg caccatcccg gaatgacgtt gctgccccgg
     3361 caccggcagc gcgcccccac aaggggagag ggagcgcgct gccgttgctg agatattgtg
     3421 gaattcttcg atgatcggga tcagcgagga ggatgggcgg agccaacggg tcgcgcgaat
     3481 gcgggcccga tgataaactc cgcgataccc atccattgca tttcgtggcg gtgatgggtt
     3541 tcgcaaagcg ctcaacccat ccggattgac aagaccggct caagtgattt gcccgtcggg
     3601 cggtttgttg acaggcgtgt cgagtcttat ttctgatttt ccgaaaaata tttctgttcc
     3661 cactcacccc gaatcaaatc tagaactcat tccgtcctcc ccacagaggg gcgtatcgcg
     3721 atcgtcacgg acgcggggtg ggatgcggtg gacgcggcag cgttggcgcg cgatggggtc
     3781 gcagggcagg tttaaaagcc tgtgagcgac acctgagcga gcagacgaac gatgctgatg
     3841 cggacggcga agtcgtgtgg tcctgacgcc ccgacgctgg cgtcaagttt gcggatgttg
     3901 tgtcggccct accgggtgcg gacaagacgt tagtccgcaa gcgacggtgg caagagagcc
     3961 cggtcaccgg ggagagcacg aaggaaaccg ttaaaaccat tgcgtgcgga atgccgggcg
     4021 attccggtgt actcgttgtt actcgtgtgc gttctaccac accaagtgca cacgaggccg
     4081 cgggtgcagc gggcacccgg cgttccccac gccctctatt ttgggcgaag attaaatgca
     4141 tcagctcggg cgcatcgcgt cgcgagcgtg aaggtgtgtc agcaaacaat gcggctgttg
     4201 aagtgggatt ccgtacgaag cattcgccgc cctcgttcct ccaaacccgc tcgtcgtgcc
     4261 cggacttgat ccgggcatcc atccctcttc gcaacaactt tcttcgaaga ggatggatca
     4321 ccgggtcaag cccggtgatc aattggtacg acggctctac aacgaattgt ttttggctgt
     4381 ttgaaattcg aatcgagaac ttgcggcaac ggcggcgcgc tccctctccc acaaggggtg
     4441 aagtgacacc gagccaacac tcgcccgtga ggccgggcag ggacggcggt gcgcacgaat
     4501 cttcagtgcg aaagagtctg caataggaaa ttcagcgccg tcgccgcaaa cacctgcatg
     4561 ttggcgacgc ggtccgcgct gccggtctcg atcgtcatcg catattgctc caggcccgcg
     4621 acgccgatgc aactgtggcc ggcggcatcg ccgtagcggt tgccggtcgg gccggtggcg
     4681 ccggtttcgg acagacccca atcggtggca aagcgcgtgc ggatctggct cgcgagcaat
     4741 ttggcgtaag cctccgaggc cgagcggatt cccttcatgg cgtcgtcggg aatgtccatc
     4801 aggatccgcc gcgcatcacg ggtatagacc acagcacccc caaggaaata agccgacgcg
     4861 ccgggcaccg cgagcagggc ggcggagatc agaccgccgg tcgaggattc cgcgaccgcg
     4921 atggtctggc gacgctcaac cagtttcgcc gctatttttt cggcaatcgc aacgagatcc
     4981 tgcatcacgc acccttcttc ggttggtcgg tgagcgaatg gtagaccacg cgaacaacgg
     5041 ccatgctcag ttttgtcatt cgggtccccc agcgacgatg ttgcgatgtc ctagcacagg
     5101 attgcatggc tgccgactcc ggctgcatcg gcccgccaat ggacgggaga gcccgcgcgc
     5161 catatgctat cccggttcac gcgctgaaga cgcggagcaa gaagaaacgc aggaggaagt
     5221 ttacgtcatg acgtcaccga tcgcgggcgg cgtggattgc gatctgcatc cgaccgtgcc
     5281 gcatctgacc agcctgctgc cctatctcaa cgattactgg cgcgatcagg tcaccacgcg
     5341 gggcatgacc gatctggtgt cgcaatccta tccgacccat tcgccgatca gttcgcggcc
     5401 ggactggcgg ccggcgcagg gcaagccggg ctccaaccct gcagacatga aggcgcaggc
     5461 gctcgaccgg tttggggtca gctacgccat ctgcaatccg ctgtacggcg tgcagatggt
     5521 gttttcggag gatatggcgg atgcgttctg ccgcgcgctc aatgactggc tggtgaagga
     5581 atggctcgac cgggacgcgc gattgcgcgg ctcgatcgtg ataccggtgc agagcatcga
     5641 gaaatcggtc gcggagatcg agcgctgcgc cgtggacaag cgttttgtgc aggtgctgat
     5701 gctggtcatg ggcgacatgc ccttgggcaa gcgggcctac tggccgatct acgccgccgc
     5761 cgagcggctg ggcctggccg tcggcatcca cgccggcagc aattatcata atccacccac
     5821 atcagtgggc tggggttcct atcacatcga ggattacgtc gcgcaggcgc aggcgttcca
     5881 gacccaactg accagcctga tcgtcgaggg cgtgttcgca aaatatccca agctgaaaat
     5941 ggtgatgctg gagtccggct ttacctggct gccgtcctat ctgtggcggc tgcacaagtt
     6001 ctggcgcggg gtgcggatgg aaacgccctg ggtggatcgc gcgcccctgg aaattgtgcg
     6061 cagcaacatc cggttttcat tgcagccgat cgacgcgccg ccggatccgg caaccctgaa
     6121 ccgcctgttt gaccatatgc agtctgatga attgctctta ttctcgaccg actatccgca
     6181 ctggcaattc gacggcgacg cggtgctgcc ggagggaatg tcgcccgatc tcgtgcgcaa
     6241 gatcatgatc gacaatccgc tcgcgacgta cggccgcctg aaagagccag cgcacgggca
     6301 tgatcccgaa aagcatgccc tcgggccacg acccgatggg tggaatccgg ttttcggaaa
     6361 agatcatgcc caaacaagat aatttcgacg agtctaattc agcgaagctg gattagactc
     6421 tggagaagga gacgacgcca tgaatgtgca gttccgcagc ggcgctgaac ccgcttcatc
     6481 cgttggcgtc aaaacggcga ttgccgattg cgatatccat ccggcgcgcg cgacggcgac
     6541 ggagctttat ccgtacctcg caaaacgctg gcacgcgcat ctcgaaacct acggcaagca
     6601 tccctatcag ggcatgatgg aaggcccgcc ctatccgaag gcgcagccca acgcgtcgcg
     6661 ccgcgacgcc tggccgccgg acggcgggcc gcagggctcg tcgctgtcgt tcatgcaaca
     6721 acagcatctc gacccgaaca atgtcgcgct cggtgtgctg tgtccgctca ataccgggca
     6781 gggcattcgc aaccaggatc tgagcgcggc gctggcgacc gccatcaacg actggcagat
     6841 cgacaaatgg accagccagg atccgcgttt gaaaggctcc atcgtggtcg ccaatgaaga
     6901 cggcccggcc gctgccgccg aaatccgcaa gcgcgccggg gacaagaatt ttgtccaggt
     6961 gctgctcttg agccgcaatg tcgagccgct cggacaacgc cgctactggc cgatctacga
     7021 ggcggcggag gaggcggggc ttccgatcgg cgtgcacgcg ttcggttttg gtggcaatcc
     7081 aatcaccgca tcgggctggc cgagttttta tatcgaggag atggtcggcc attcgcaatg
     7141 ccagcagacc gcgctcgcca gcatcgtgct ggaaggcgtg ttcgagcggc acccgaaact
     7201 caagatggtc atgatcgagg ccgggtttgg ctgggcgccg tcgctgtcgt ggcggctcga
     7261 caagagctgg caacggctga agagcgaggt gccgcacgtc aagcgcccgc cctccgaata
     7321 tatccgcgac cacattttct ggaccacgca gccgatggaa gaccctgagc ggcgcgatca
     7381 tctgttcgac gtgatcgact gggtcggctg ggacaaattg ctgttcgcga ccgactatcc
     7441 gcattgggac tatgacgaac cgtcgcgggt gctgccggcc ggcgtcagcg acgccaaccg
     7501 cgaagcgttc tatctcggca atgcgcggaa gctgtacggg ctttcttgat ggttcgtcat
     7561 gtcatcgccc cggtggatga gctgccgccg ggcacccgaa aatttctcac catcgatgcg
     7621 cgcccgatcg cggtgttcaa catcaagggc gagttcttcg gcctgttgaa ccgatgcccg
     7681 catcaaggtg ctgcactgtg cgaggggccg ctgattgggc tggcgcaatc gtccgatccc
     7741 ggcgagatcg aatataccag gttaggcgaa atcatccgct gcccctggca cggctgggaa
     7801 ttcgacatcc gcaccggcca atcctactgc gaccccaaac ggtttcgcgc caaaacctat
     7861 cccgccaatg tcgagccggg gtcgagcgtg gtgaagggac cttacgtggc ggagacgatc
     7921 gcggtgtcgg tcgagagcga ttatgtggtg gtggatttgt gaatacgccg tcacgcccac
     7981 gctgtttacc gtcattgcga ggagcgaagc gacgaagcaa tccattctct gctgctcgtg
     8041 gattgcttcg cttcgctcgc aatgacggcg ctaaacatgc cccagcatcc gccgctcccg
     8101 cgcatagcgc accagcgcga cgtagcgata gatgccatgc acgaacttgc cgtagggcat
     8161 ggtgatgaac agggaaaaca cgacgccgag atgcagcgcg agcagcggcc ccatcgcggc
     8221 cgtatcgcgc aacagcaaca gtgcgatgcc cgtcagactg gtcaggaaca gcatggcgat
     8281 gaacgcgacg tccatgccgc tgcgcttctc gtcgcccagc gccggatcgc gcttccattt
     8341 ttccttaagc aggccggcgg gtccgatcag aagcccgatg ccgccgagcg tccccaagac
     8401 caccggcagg tcccaccacg gatacggcgc ctcgcgcgcg atcaaataat gatacagcgt
     8461 cgccacgcat gtcgcagcga agcacagcaa gaaaccgtag aacgtaaaat ggtgaaagat
     8521 cttgcggcgg tcgctcggcc gttcgtcctc attgacgcag ccgacgccgc cgccatcgag
     8581 atagcgcagt tggcctgcat ccatcatcgc ctgccagagc gagggcggat ccaccagcat
     8641 acggatcggt tcgccgatat cgcgccagaa agcgcgcaca cccatcgcca gtgccgcgat
     8701 cgcatagagg aacgccgcgc cgaacaacgc cgccatcgcg ttatgcggca tcagcgcata
     8761 gaacgcgccg ggcccggtat ggatgcccag cagaacctgg cggtcgttaa gggccgcgaa
     8821 gccgaaaata aatg
//
LOCUS       DATNOI010000466         2456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_889706,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000466 DATNOI010000000
VERSION     DATNOI010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889706"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1128)
                     /locus_tag="VN968_18555"
     CDS             complement(181..1128)
                     /locus_tag="VN968_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL31273.1"
                     /translation="MTLVSIPANPVPEDVVAGTIKTPDGAELRFARWAPPAGRKGTVC
                     VFTGRGEQIEKYFETVRDLRDRGFAVAMIDWRGQGHSSRRLRDPRKGYVRDFSDYEVD
                     VDTFVQQVVLPDCPPPHFALAHSMGGAVMLRVAHAGKRWFDRMVLTAPMIDLPGHRTG
                     FAARALLRFLRLTGQGGRYIPGGSDELVGASSFVNNPLTSDPVRYARNAAILEEDPTL
                     GIGSPTVAWADTAFKAMRGFRASDYPLQIRQPILMLAASNDSVVSTAAIEEFAYHLRA
                     GSHLVIAGSKHEILQEQDRYRGQFWAAFDAFVPGTPLFK"
     gene            1362..1841
                     /locus_tag="VN968_18560"
     CDS             1362..1841
                     /locus_tag="VN968_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HXL31274.1"
                     /translation="MRTYDLTPFYRSTVGFDRLFSLLDQATADGSPGYPPYNIERTGE
                     NAYRISVAVSGFAPDELSIVAKENTLTIKGDKTANENGKDKSEVLYRGIAARAFERAF
                     QLADFVQVRNASLENGLLHVDLVREIPEAKKPRSIPITTTSAVAPQVVDNSAEKAAA"
     gene            2108..>2456
                     /locus_tag="VN968_18565"
     CDS             2108..>2456
                     /locus_tag="VN968_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL31275.1"
                     /translation="MKITRVVIALTVVALAATLGGFDARAQTAAPTEARNVVLVHGAW
                     ADGSSWAAVIPYLQAAGLKVTAVQNPLTSLEDSVAATRRALALQDGPTVLVAHSWGGT
                     VISETGIDPKFTAL"
BASE COUNT          478 a          815 c          741 g          422 t
ORIGIN      
        1 gtcgagaaag tgcgcgctga gtgggcgatg atctgcaccg cccacaacct cctgaagctg
       61 ttcaccatcg caatcgccgc ttgagccggc taccctgcaa caaatgtccg tcacaaaact
      121 tatctggacg ggctcctagg cctgctgagg cgccttcgac gcgatcacga tcgcctcgtc
      181 tcacttgaac agcggcgtgc ccggcacgaa ggcatcgaac gccgcccaga attgcccgcg
      241 gtagcggtcc tgctcctgca ggatctcgtg tttcgaaccg gcgatcacca gatgcgagcc
      301 ggcgcgcaag tgataggcga actcctcgat cgccgccgtc gaaacgacgc tgtcgttgct
      361 ggcggcgagc atcaggatcg gctgacggat ttgcaaagga tagtccgaag cccgaaatcc
      421 gcgcatcgcc ttgaacgcgg tatcggccca ggccaccgtc ggcgacccga tgccgagcgt
      481 cggatcttct tccaggatcg cggcattgcg ggcgtagcgc acgggatcgc tggtcagggg
      541 attgttgacg aacgaggatg cgcccaccag ttcgtcgctg ccgccgggaa tgtagcggcc
      601 gccctgcccg gtgagccgca ggaatcgcag cagtgcccgc gcggcaaatc cggtgcggtg
      661 gccgggaaga tcgatcatcg gcgccgtcag caccatgcgg tcgaaccagc gctttcccgc
      721 atgcgcgacg cgcagcatca cggctccgcc catcgaatgc gccagcgcga aatgcggcgg
      781 cgggcaatcc ggcagcacca cctgctgcac gaaggtatcg acgtcgactt cgtaatcgga
      841 gaaatcgcga acgtagccct tgcgcgggtc gcgcaggcgc cgcgacgaat ggccctgccc
      901 gcgccaatcg atcatcgcca ccgcgaaacc gcgatcgcgc aaatcgcgca ctgtttcgaa
      961 atatttttca atctgctcgc cccgccccgt gaagacgcag accgtgccct tgcggcccgc
     1021 cggcggcgcc caccgcgcga accgcaattc ggcgccatca ggtgtcttga tagtgccggc
     1081 gacgacgtcc tccggaacgg gattggcggg gatcgagacg agcgtcatga atgcgaccgg
     1141 ctatgtcaaa agtgctgaaa atcaagggcg ggcaagcggc gggacgtcaa aaaacggctg
     1201 cgtcgccgcc tcttgaacac caacaggccc ctcccatatc accttcgtgc aggccgctac
     1261 cagcgtttcg gggaaccgga acacaaggct gcacagtttc gaaagcccgg cccgatggcg
     1321 ggcgggttca attcaacagt cgctcaatgg aggactatcc tatgcgtacc tacgatctca
     1381 ccccatttta tcgttccacc gtcggcttcg accggttgtt ttccctgctc gaccaggcga
     1441 ccgcggacgg cagccccggc tatccgccct acaatatcga gcgcaccggc gagaacgcct
     1501 atcgtatcag cgtcgcggtc tccggcttcg cgccggacga gctctccatc gtcgccaagg
     1561 aaaatacgct gacgatcaag ggcgacaaga ccgccaatga gaatggcaag gacaagtccg
     1621 aggtgctgta tcgcggcatc gcggcgcgcg ccttcgagcg ggcattccag cttgccgatt
     1681 tcgtgcaggt gagaaacgcc tcgcttgaga acggcctgct tcacgtcgat ctcgttcgcg
     1741 agattcccga agccaagaag ccccgcagca tcccgatcac gaccacgagc gccgtcgctc
     1801 cgcaggtcgt cgacaactcg gccgagaaag ccgccgcgta aacgcgggtt ggtttgctga
     1861 acgcgaaaac gccccgggaa accggggcgt tttttccgtc gtcattccgg ctcgcgcttc
     1921 gcgcgccccg gaatgacgat gtagtttctc tagcccccgt cgttcgaatc cagtttccgc
     1981 tccagtcact gcgcgcgacg tctttcgaaa ttcacagagc tgtgaacccc cgtaacgttg
     2041 cgaccccatc ggccgaaaaa cattatggag cgcaacgctc ggaagttttg gccatggagt
     2101 tggtatgatg aagataaccc gagtcgtgat tgcactcacc gtcgtcgcgc tcgcggcaac
     2161 gctcggcgga tttgacgcgc gcgcgcaaac ggccgcgccc accgaggcgc gcaacgtcgt
     2221 gctggtgcac ggggcatggg cggacggctc cagctgggct gcggtcattc catatctgca
     2281 ggcggccggt cttaaagtca ccgccgtgca aaatccgctg acctcgctcg aagactcggt
     2341 tgccgcgaca cgccgcgcgt tggcgctgca ggatggcccg accgtgctgg tcgcccactc
     2401 ctggggcggc accgtcatca gcgagaccgg gatcgacccg aaattcaccg ccctag
//
LOCUS       DATNOI010000467         5532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_210653,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000467 DATNOI010000000
VERSION     DATNOI010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210653"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VN968_18570"
     CDS             complement(<1..886)
                     /locus_tag="VN968_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXL31276.1"
                     /translation="MPNTPTLATLADDLDSGRTSARKLVDECLARIADTSGEGMRAFI
                     HVDTEAAIEAAEAMDRLREVKAAPSPFAGIPVSIKDLFDIKGQVTRAGSRALDDSAPA
                     EADASAVARLRRAGFIVIGRTNMTEFAYSGIGINPHYGTPKGAWNRREGHVPGGSSSG
                     AAVSIADRMAYGALGTDTGGSCRIPAAFNGIVGFKPTQRRVPLDGGVPLSSSLDSFGP
                     LARTAQCCATLDAVLADEPEQPVQARPVKGMRLAVPTTITLDDLDDAVAKTFERALQS
                     LSRAGALIEHIEMPEFLDV"
     gene            complement(913..1236)
                     /locus_tag="VN968_18575"
     CDS             complement(913..1236)
                     /locus_tag="VN968_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31277.1"
                     /translation="MTKLREIAHSRTGDKGNTSNISVIAYDAKHYPLLLAQVTSERVR
                     AHFAGVVAGEVVRYELPNIAALNFVMAGALGGGVTRSLALDAHGKSLSSALLDLEIEA
                     APDNE"
     gene            complement(1233..2582)
                     /locus_tag="VN968_18580"
     CDS             complement(1233..2582)
                     /locus_tag="VN968_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HXL31278.1"
                     /translation="MRTIRIGSGAGYSGDRIEPAVELAEKGDIQYLVFECLGERTVAL
                     AQQARMKNPDGGYDPLLEERMRAVLPICAPKGIKIVTNMGAANPVAAARKTAEIARSL
                     GFASLKIAAVIGDDVLDACKDGDLEIMEFDGTIKQLGNRLLSANAYLGAEPIAEALTG
                     GADIVITGRASDPALFLAPMIHAFGWAMDDWNLLGQGTVAGHLLECAGQITGGYFADP
                     GYKDIPDLARLGFPIGEVGEDGSLVITKVAGSGGAVTAQTCKEQLLYEVHDPKRYIQP
                     DVVADFSQVKVEEIAPDRVRVSGGRGTTRTDTLKVSVGYVDSFIGEGQISYAGPGALA
                     RGRLALDIVRERLKLTGVASSELRFELVGVESLHGAEVSARAMPNEPYEVRVRVVGRT
                     ENLREAIRIGNEVETLYTNGPAAGGGAFKSARDVVAVASVLLSRELAKPSIQFVGRE"
     gene            2816..3229
                     /locus_tag="VN968_18585"
     CDS             2816..3229
                     /locus_tag="VN968_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HXL31279.1"
                     /translation="MAEPTRARPKPTPETQHFWDGTRAGELRLQRCDACANVYFPPRP
                     FCPACASREVSMFKASGKATLYSYVINHRPAAPGFTPPYAIAVVELEEGPRMMSNIID
                     CPQTPEALELDMKLEVAFEKLDDKITLPLFRPAKG"
     gene            3235..4374
                     /locus_tag="VN968_18590"
     CDS             3235..4374
                     /locus_tag="VN968_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HXL31280.1"
                     /translation="MRRNQVAVVGAAETTELGVIPNVSQIQLHADAALNALADAGLKL
                     SDIDGIATAVETPQQIAHYLGITPTWVDGTSVGGCSFMLHVRHAAAAIEAGLCKTVLI
                     THAESGKSMIGKQPRSTPADSLNGQFEAPFGVYGPPSMFPIPVLRYMKTHGITHEQIA
                     MVAVVQREWAAKNPRATMKAPITVEDVLNSRMIAYPFRILQCCLVTDGGGALILTSAD
                     RAKDFKTRPVYILGSGESVETPMVSQMQTFDSSRAFRVAGQLAFKEAGITHGDVDHLM
                     IYDAFAHLPLYGLGDLGFMPHEETGKFIADGNTRPGGKLPLNTNGGGLSYMHSGMYGM
                     YALQESVRQMRGIAPAQVPGAKISVCHGVGGMFGASGTIIFTNER"
     gene            4624..>5532
                     /locus_tag="VN968_18595"
     CDS             4624..>5532
                     /locus_tag="VN968_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL31281.1"
                     /translation="MAKSLQDKVVIVTGAGRGIGREIALLCAAEGAKVVVNDPGVAAD
                     GSGTSAAPAEEVVEEIKKRGGTAVANFETVAEAVPASKIVKMAVDTYGKLDGVVNNAG
                     ILRDAIFHKMSVDAFESVIKVHLMGSFYVAHAAARLFREQESGSFVHFTSTSGLVGNF
                     GQANYAAAKLGIVGLSKSIALDMNRYNVRSNCVSPFAWSRLIGTIPTETEAEKARVAK
                     IQQMGPEKIAPVCAFLLSDAAKDVTGQIFAVRMNEIFLMSQSRPLRSVHRAEGWTPQT
                     LAEHGIPALKSSFYKLDRSADVFNWDA"
BASE COUNT         1025 a         1806 c         1729 g          972 t
ORIGIN      
        1 cgacgtccag aaattccggc atctcgatgt gttcgatcag cgcgccggcc cgtgaaagcg
       61 actgcagcgc gcgctcgaac gtttttgcca cggcatcgtc gaggtcgtcg agcgtaattg
      121 ttgtcggcac cgccagccgc attcccttga cgggccgcgc ctgcacaggc tgctcgggct
      181 cgtcggccag cacggcgtcg agcgtcgcac agcactgcgc ggtgcgcgcc agcggtccaa
      241 aactgtcgag cgaggatgac agcggcacgc cgccgtcgag cggcacgcgg cgttgcgtcg
      301 gcttgaaacc gacgatgccg ttgaaggccg ccggaatccg gcaggagccg ccggtgtcgg
      361 tgccgagcgc gccataggcc atgcgatcgg cgatcgacac cgccgcgccc gacgaggaac
      421 cgccgggcac atggccttcc ctgcgattcc acgcgccttt cggcgtgccg tagtgcggat
      481 tgataccgat gccggaatag gcgaactcgg tcatgttggt gcggccgatc acgatgaagc
      541 cggcgcggcg caaccgcgcc accgccgatg cgtcggcttc cgccggggct gaatcgtcga
      601 gcgcgcgcga accggcgcgg gtcacctgcc ccttgatgtc gaacaggtcc ttgatcgaaa
      661 ccggaatgcc ggcgaatggc gacggcgccg ctttcacctc gcgcagccgg tccatcgctt
      721 ccgccgcttc gatggcggct tccgtatcga catgaataaa ggcccgcatc ccctcgcccg
      781 acgtgtctgc gatcctggcg agacattcgt cgacaagctt gcgggcactg gtgcggccgc
      841 tgtcgagatc gtcagccagc gtggcaagtg tcggagtgtt tggcatcgta gtttcgcctc
      901 gtggctgctt gattattcgt tgtccggagc cgcttcgatt tccagatcga gcagcgccga
      961 gctcagggat ttgccgtgcg cgtccagcgc cagcgatcgc gtcaccccgc cgccgagcgc
     1021 accggccatc acgaaattca acgcggcgat gttgggcagc tcatagcgga caacctcgcc
     1081 tgcgacaaca ccggcgaaat gcgctcttac ccgctcgctg gtgacctgcg cgagcagcag
     1141 cggataatgc ttcgcatcat aggcgatcac ggagatgttg gaggtgttgc ccttgtcacc
     1201 ggtgcgggaa tgggcgatct cgcgcagttt tgtcattcac gccccacaaa ctggatggat
     1261 ggtttcgcca gttcgcgcga cagcagcacc gacgccaccg cgacgacatc acgggcggat
     1321 ttgaacgcgc cgccgccggc ggcgggaccg ttggtgtaga gcgtttcgac ttcgttgccg
     1381 attctgatcg cctcgcgcag gttttcggtg cggccgacaa cgcgaaccct cacctcgtaa
     1441 ggttcgtttg gcattgcgcg agcggaaacc tcggccccgt gcagcgactc gacgccaaca
     1501 agctcgaagc gcaattcgct tgatgcaacg ccagtcagtt tcaaccgttc ccgaacgatg
     1561 tccagcgcca gccgcccgcg tgccaatgcg ccgggcccgg cataggaaat ctgcccctcg
     1621 ccaataaaac tgtcgacata gccgaccgag accttcagcg tgtcggtgcg cgtggtgccg
     1681 cgcccgccgc tgacgcgcac gcgatccggt gcgatctcct cgaccttcac ctgtgaaaaa
     1741 tccgcgacca cgtcgggctg aatatagcgt ttgggatcat gcacctcgta gagcagctgc
     1801 tccttgcagg tctgcgccgt caccgcgccg ccggagcccg cgaccttggt gatcacaagg
     1861 ctgccgtctt cgccgacctc gccgatcgga aagccgagcc gtgccagatc ggggatatcc
     1921 ttgtagccgg ggtcggcgaa gtaaccgccg gtaatctggc ccgcgcattc cagcagatgc
     1981 ccggcgactg tgccctgccc gagcagattc cagtcatcca tcgcccagcc gaatgcatga
     2041 atcatcggcg ccagaaacag cgcgggatcc gatgcacgcc cggtgatcac gatatcggcg
     2101 ccaccggtca gcgcctccgc gatcggctcg gcgccgagat aggcgttggc cgacaatagc
     2161 cggttaccga gctgtttgat ggtgccgtcg aactccatga tctccaggtc accatccttg
     2221 caggcatcga gcacgtcgtc gccgatgaca gcagcgatct tcagcgacgc gaacccgagc
     2281 gatctcgcga tctcggcggt cttgcgcgcc gcggcaacgg gattggccgc gcccatattg
     2341 gtgacgatct tgattccctt aggcgcgcag atcggcagca ccgcgcgcat ccgctcctcg
     2401 agcagcggat cgtagccgcc gtcgggattt ttcatgcgcg cctgctgcgc cagcgcgacg
     2461 gtgcgctcgc ccagacactc gaataccaga tactggatat cgcccttttc ggccagttca
     2521 accgcgggct cgatgcgatc gcccgaatac ccggcgccgg atcctattcg aatcgttcgc
     2581 accctctgcc tcccgcgcag cgtcgctatt cccagtgagg agttgccgca atgacaaaca
     2641 ccctagcgcg ggagatcgaa agcatacaag cgttccggga gacggattgt gcgcgtgccg
     2701 gaatacgcgc catgcagcag gtcggctgga ccgttgacgc cccatggtca aatgtcgcat
     2761 caagaccgca acagcgggcc ccgcggcccg atccccaggg gaggaaatgg cagacatggc
     2821 cgagccaacg cgcgcgcgac cgaaaccgac accggaaacc cagcatttct gggacggcac
     2881 gcgggcaggc gagctgcgcc tgcagcgctg cgatgcctgc gccaatgtct atttcccgcc
     2941 gcgaccgttc tgcccggcct gcgcctcgcg cgaggtcagc atgttcaaag ccagcggcaa
     3001 ggcgacgctc tacagctacg tcatcaacca tcggcctgcc gcgcccggtt tcacgccgcc
     3061 ttacgcgatt gccgtggtgg aattggaaga aggcccgcgg atgatgagca acatcatcga
     3121 ttgtcctcag acgccggagg cgctcgaact cgacatgaag ctcgaagtcg cgttcgaaaa
     3181 gctcgacgac aagatcaccc ttcccttgtt ccgtccggcg aagggctaag catcatgcgc
     3241 agaaaccagg tcgccgttgt cggcgctgcc gagaccaccg aactcggcgt catcccgaac
     3301 gtctcacaga tccagttaca cgccgacgcg gcgctgaacg cccttgccga tgccgggttg
     3361 aaactgtccg atatcgacgg catcgctacc gcggtcgaga cgccgcaaca gattgcgcat
     3421 tacctcggca tcacgccgac ctgggtcgac ggcacgtcgg tcggcggatg ctcgttcatg
     3481 cttcacgtcc gccacgcggc ggccgcgatc gaggccgggc tttgcaaaac cgtgctgatc
     3541 acccacgccg agagcggcaa gtcgatgatc ggcaagcagc cgcgttcgac gccggcggac
     3601 agcctgaacg gccaattcga agcgccgttc ggcgtctacg ggccgcccag catgtttccg
     3661 atcccggtgc tgcgctacat gaagacccat ggcatcaccc atgagcagat cgcgatggtc
     3721 gcggtggtgc agcgtgaatg ggccgcgaag aatccgcgcg ccaccatgaa ggcgccgatc
     3781 acggtcgagg atgtgctgaa ctcgcggatg atcgcctatc cgttccgcat cctgcagtgc
     3841 tgcctcgtca ccgacggcgg cggcgcgctg atcctgacct cggccgaccg cgccaaggat
     3901 ttcaagacca ggccggtcta tatccttggg tcaggcgaga gcgtggaaac gccgatggtc
     3961 agccagatgc agacgtttga cagttcgcgc gcgttccggg tcgcgggcca gctcgccttc
     4021 aaggaggccg gcatcaccca tggggacgtc gaccacctga tgatctacga cgcctttgcg
     4081 catctgccgt tgtatggcct cggcgatctc ggcttcatgc cgcacgaaga gaccggcaag
     4141 ttcatcgccg acggcaacac caggcccggc ggcaagctgc cgctgaacac caacggcggc
     4201 ggtctcagct acatgcattc cggcatgtac ggcatgtatg cgctgcagga gagcgtgcgg
     4261 caaatgcgcg gcatcgcgcc ggcgcaagtg ccgggcgcga agatttcggt ctgccacggc
     4321 gtcggcggca tgttcggggc aagcggcacg attattttta cgaacgagag atagagaacg
     4381 cgaaccgctt ggacgaccga actttaccgc tgtcattccg ggatgcgcca cccgaactcg
     4441 ggtttacccg agttcggcaa tatcattgtc caagtcggca acagcggact tggatgcgca
     4501 ggcccggaat ccatactccc tgcaggggtt atggattccg ggctcgctcg tttcactcgc
     4561 gccccggaat gacgtttgtt gtttgataga gcgtgtagcc aatcggtcac gcaggagagc
     4621 aatatggcaa aatcactgca ggataaagtc gtcatcgtca ccggcgctgg gcgcgggatt
     4681 ggccgcgaga tcgcgctgct ctgcgccgcg gaaggcgcca aggtcgtggt caacgatccc
     4741 ggtgtcgccg ccgacggttc gggaacgagc gccgcgccgg cggaagaggt ggtcgaggaa
     4801 atcaagaagc gcggcggcac ggccgtggcc aatttcgaaa ccgtcgccga ggcggttccc
     4861 gccagcaaga tcgtcaagat ggcggtcgac acctatggca agctcgacgg cgtggtcaac
     4921 aatgccggca tcctgcgcga cgcgatcttc cacaagatga gcgtcgacgc cttcgagtcc
     4981 gtgatcaagg tgcacctgat gggttcgttc tatgtcgcgc acgcggcggc gcggctgttc
     5041 cgcgagcagg aaagcggctc gttcgttcac ttcacctcga cctccggcct ggtcggcaat
     5101 ttcggccagg ccaattacgc ggccgcaaaa ctcggcatcg tcgggctgtc gaaatcaatc
     5161 gcgctcgaca tgaaccgcta caatgtgcgc tcgaattgcg tctcgccgtt cgcctggagc
     5221 cggctgatcg gaaccattcc gaccgagacc gaggccgaga aagcgcgggt ggcgaaaatt
     5281 cagcagatgg gaccggaaaa gatcgcgccg gtttgcgcct tcctgctcag cgacgccgcc
     5341 aaggatgtca ccgggcagat ttttgcggtg cggatgaacg agattttcct gatgagccag
     5401 tcgcgcccgc tgcgctcggt ccatcgcgcc gaaggctgga cgccgcagac cctcgccgag
     5461 cacggcatcc cggcgctgaa gtcgtcgttc tacaagctcg accgctcggc ggatgttttc
     5521 aactgggatg cg
//
LOCUS       DATNOI010000468         6321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_577447,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000468 DATNOI010000000
VERSION     DATNOI010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577447"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VN968_18600"
     CDS             complement(<1..983)
                     /locus_tag="VN968_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HXL31282.1"
                     /translation="MSRASSRALKHPPVTAPSEPAGAPSPFPELSVAIERERRHGRGA
                     QSNDSGRFEAEARVAFDDGWQSLDELPPFKTTVAVDTARKVITRNDSPDIGFDRSINP
                     YRGCEHGCVYCFARPTHAFLGLSPGLDFESKLFAKPDAPALLEKELAAQDYEPRMIAI
                     GTNTDPYQPIERERKIMRGILEVLERASHPVGIVTKSALVTRDIDILSRMAKRNLAKV
                     ALSVTTLDSKLARTMEPRASMPTRRLEALRQLSEAGIPTTVMVAPVIPALNDSEIERI
                     LDAAAHAGVKEASYVLLRLPLEVRDLFREWLMANYPDRYRHVFTMIRDMRGG"
     gene            1285..1491
                     /locus_tag="VN968_18605"
     CDS             1285..1491
                     /locus_tag="VN968_18605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31283.1"
                     /translation="MIKQSSASAPKTAEVDILIIPISEETTIGRHCSCKTVRELEDLR
                     RPLPRSGTTSPVTLDWRKKLQTAG"
     gene            1557..2126
                     /locus_tag="VN968_18610"
     CDS             1557..2126
                     /locus_tag="VN968_18610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HXL31284.1"
                     /translation="MLSVIIPTEGVEQPAVATLAALVPGAAAGVIREVLLVDRTGTGV
                     IERVADVAGCRFLRFEGSHAAALAAGARQARSPWLMFLHAGAVLDSGWIEETTQFIQG
                     VSNSGRPRAGIFRYARSPYAETRLRDGFKFVARMIAGPSADQGLLIARDHYERLGGYA
                     PDARRSEARLLRQLGRSSRTLLRSRIMVA"
     gene            2192..3121
                     /locus_tag="VN968_18615"
     CDS             2192..3121
                     /locus_tag="VN968_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HXL31285.1"
                     /translation="MPQQDSDDQISAVRSFNRFYTRKLGVLDQQLLKTPFSLSEARVL
                     YELANGENLAAKEIGIELGLDPGYLSRIIQNFDENGLIARQPLPSDRRQYQLSLTAKG
                     RQAFAKLDRSSYDDVAAMLATLPRGDSERLIGAMAVIERLLGAPSVSPRPAILRGPRP
                     GDMGWVVQSHGSLYASEYGFDSSFEGLVAEIAAKFLSSYDASRERCWIADIDGAQVGS
                     IFLVRHTDDVAKLRLLLVDPAGRGQGLGQRLVAECVAFGRQCGYRKITLWTQSILVAA
                     RRIYQDAGFVLVATEPHRSFGQSLIGETWEREL"
     gene            complement(3773..4336)
                     /gene="moaB"
                     /locus_tag="VN968_18620"
     CDS             complement(3773..4336)
                     /gene="moaB"
                     /locus_tag="VN968_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HXL31286.1"
                     /translation="MASIDESKQFVPLNIAVLTVSDTRSLEDDKSGTTLSERLTAAGH
                     KLAAREIIVDEVDAIRLIIKRWIADPGIDAIITTGGTGFTGRDVTPEAIEPLFEKRMD
                     GFSIAFHMLSHAKIGTSTIQSRATAGVAGATFIFCLPGSPGACRDGWDGILAAQLDYR
                     TRPCNFVEIMPRLDEHLRRPKAQGASA"
     gene            complement(4489..5415)
                     /locus_tag="VN968_18625"
     CDS             complement(4489..5415)
                     /locus_tag="VN968_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HXL31287.1"
                     /translation="MRYDDFRRSDDIDDRRDDSGGGGGGGFGLPMGGGGLGIGTMIVL
                     GLVGYAFGIDPRILIGGAEILTGGQSPSYQSDRRSAPAKTGAPTDEMGSMISGVLGEI
                     DDRWSEIFQASGQSYSGPRIVLFRNATNGGRCGMAQAAMGPFYCPPDKQIFLDTGFFR
                     QVETRFHGCSGNACKFTAAYIIAHEAGHHIQNLLGILPRVTRMQQQAGSKAEANALQV
                     KVELQADCLSGVWVNREEKKRPGFLEAGDIDAALTTATAIGDDTLQRQATGRVVPDSF
                     THGSAAQRKQWFMTGYQQGTVQACNTFGAGTL"
     gene            5653..>6321
                     /locus_tag="VN968_18630"
     CDS             5653..>6321
                     /locus_tag="VN968_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HXL31288.1"
                     /translation="MVVSRRGASARAPRTQFESDPSTRIVVGDCVAEMSKLPAGSVDL
                     VFADPPYNLQLKGDLKRPDESHVDAVNNDWDKFSSFAAYDDFTRAWLLACRRIMKPSA
                     TLWVIGSYHNIFRVGSIMQDLGFWVLNDIVWRKTNPMPNFRGRRFTNAHETMIWAARD
                     EKAKGYTFNYEALKAANEDVQARSDWLIPLCTGDERLKGEDGKKVHPTQKPEGLLARV
                     LLSSS"
BASE COUNT         1213 a         1962 c         1944 g         1202 t
ORIGIN      
        1 ccgccgcgca tgtcgcggat catggtgaag acgtggcgat agcgatcggg gtaattcgcc
       61 atcagccatt cgcggaacag gtcgcgcacc tccagcggca accgcagcag cacgtagctc
      121 gcttccttga cgccggcatg cgccgccgca tcgagaatgc gctcgatctc ggaatcgttc
      181 agcgcgggga tcaccggcgc caccatcacc gtggtcggaa ttccggcttc ggacagttgc
      241 cgcagcgctt cgagccgccg cgtcggcatc gaggcccgcg gctccatggt gcgcgcgagt
      301 ttcgaatcga gcgtcgtgac cgagagtgcc accttggcga gattgcgctt tgccatccgc
      361 gagagaatgt cgatgtcacg cgtcaccagc gccgacttgg tgacgatgcc gaccgggtgc
      421 gaggcgcgct ccagcacttc gagaatgccg cgcatgatct tccgttcgcg ttcgatcggc
      481 tgatagggat cggtgttggt gccgatcgcg atcatgcgcg gttcgtaatc ctgcgccgcc
      541 agttcctttt ccaacagcgc cggcgcgtcg ggcttggcga acagcttgga ttcgaagtcg
      601 agccccggcg acaggccgag aaaagcgtgg gtgggccgcg caaagcaata gacgcagcca
      661 tgctcgcagc cccggtaagg gttgatcgaa cggtcaaaac cgatatcggg agagtcgttg
      721 cgggtaatga ccttgcgtgc ggtatcgacc gcgaccgtgg tcttgaatgg cggcaattca
      781 tcgagactct gccagccgtc gtcgaacgcg acccgcgctt cagcctcgaa gcggccgctg
      841 tcgttggatt gcgcgccgcg gccatgtcgc cgctcgcgct cgattgcgac cgacaattcg
      901 ggaaagggag aaggcgcacc cgccggttcg gagggcgccg tgaccggcgg gtgcttgagg
      961 gcacgagagg atgctctgct catggtgaga acgtaacaca ccatgagaac aaatcaagaa
     1021 cgaatcttcg gcgataatcg aatttggctg gtccgctgcc aatcgggttc ctgcttgagg
     1081 ggaatcaata gctggcgggt tcgtccaagg caaaattaga gatggcaggc ggcgaattca
     1141 acgaaacgtc ggcagcccct tgagttcgat cacccaagcg ccgccgaggc catcgacctg
     1201 ttcacttttc acatcgcaag gagatacggc agcaaatatg caatcgcttg cgatggttag
     1261 ggattacaat gaatccttcc gcaaatgatc aagcaaagca gcgcatcggc gccaaaaaca
     1321 gccgaggtcg atatcctcat cattccgatc agcgaggaga cgacgatcgg gcgccattgc
     1381 tcctgcaaaa ctgtccggga gttagaagac ctgcgtcgcc ccctgccccg gtccggcacc
     1441 acaagccccg tcaccctcga ttggcgcaaa aagctgcaaa cggcaggctg atttgattgt
     1501 gacaaggtga taacagtcgg cgttttctct ccttttgagg catagaacag ccgtcaatgt
     1561 tgagcgtgat catccccacc gaaggtgtcg agcagcccgc ggtcgccacc ttggcggcgc
     1621 tggtgccggg cgccgctgcc ggcgtgatcc gcgaggtact gctggtcgat cggaccggca
     1681 ccggcgtgat cgagcgcgtc gccgacgtcg caggttgccg ctttttgcgg ttcgagggat
     1741 cgcatgcggc ggcgctggcg gccggggccc ggcaggcccg ctcgccttgg ctgatgttcc
     1801 tgcatgccgg cgccgtgctg gactcgggct ggatcgagga aaccacgcaa ttcatccagg
     1861 gggtctcgaa cagcggccgt ccccgcgccg gaatcttccg ctacgctcgc tcgccctatg
     1921 ccgaaacccg cctgcgcgat ggtttcaaat ttgtcgccag gatgattgcg gggccgtcgg
     1981 cggatcaggg attgctgatc gcacgggacc actacgagcg gctcgggggc tatgcgccgg
     2041 acgcccgccg ctccgaggcc agactgcttc gccagcttgg ccgctcctcg cgcaccctgc
     2101 tgcgcagccg gatcatggtg gcctgagccg caccgagata cttgccaaag tcaaataatt
     2161 atttgacaat ggcaactatt ggaggtgcgc catgccacag caggattccg acgaccagat
     2221 atcggcggtc cgcagcttca accgcttcta cacacgcaag ctcggcgttc tcgaccagca
     2281 gctgctgaaa accccgttct cgctgagcga ggcgcgggtg ttgtacgaac tcgccaacgg
     2341 ggaaaacctg gccgcgaaag agatcgggat cgaacttggt cttgatccag gatatctcag
     2401 ccgaattatc cagaatttcg atgaaaacgg gttgatcgcc cgtcagccgc tgccctcgga
     2461 tcggcggcaa tatcagctca gcctgaccgc gaagggacgt caggcgttcg cgaagctcga
     2521 ccgcagttca tacgacgatg tcgcggcgat gctcgccacg ctgccacgcg gcgacagcga
     2581 acggctgatc ggagcgatgg ccgtgatcga gcggctgctc ggcgcgccca gcgtttcacc
     2641 gcggcccgcg atcctgcgcg gcccccgtcc gggcgacatg ggatgggtgg tgcagagcca
     2701 tggctcacta tacgccagcg aatacggctt cgactcctcg ttcgaaggtc tggtcgcgga
     2761 gatcgcggca aaattcctca gctcgtacga cgcctcgcgg gaacgctgct ggatcgccga
     2821 catcgatggt gcccaggtcg gctcgatatt tctcgtcagg cataccgacg acgtcgctaa
     2881 actgcggttg ctgctggtcg atccggccgg acgcggacag ggacttggcc agcggctggt
     2941 cgccgaatgc gtggccttcg ggcggcagtg cggctatcgc aagatcacgt tatggaccca
     3001 gagcattctg gtcgccgccc gcaggattta ccaggatgcc ggattcgtgc tggtcgccac
     3061 tgagccgcac cgcagtttcg gccaaagcct gatcggcgag acctgggaac gcgaactgtg
     3121 acatggaggc gtggcaatgg accgcgccgc gtcaccccgg aactgatggc attttacaag
     3181 tgtagggcaa aacagctccg cgacgaagcc ttcctcgata tgggccgtgg tttatgggcg
     3241 ttcctgcaaa ggatcgtccg gcggacgttg acggcaatat cgcaatattg gcaactattg
     3301 gatcagtaac agatcagtaa cagaacgagc caacacgatg catgaagata atgcggcgtt
     3361 ctcgcaaatc gggggtgctc ggatcaattt gatgaatgtc acttatccgt acgcagagct
     3421 ttcaatcttt cgaaatgcgt tacgccttac ctgtttggat cgagagtacg tgtttctccg
     3481 gggcaatatc attgctttag cgaagtaccg aggtctgttt tccaccggtc tgagtatccg
     3541 ccacagcgtg ccaacgtatc ctgagttcat agtgttctgg gttaacattt tcggttgggg
     3601 atcccgctac gcggtgctca agaagcaact acaagcgttc ggatacgagg tgaatgagtg
     3661 agatgaagag tttgagtcga gaatcttcca ccgtgattcc ggggcgacgc caacggggtc
     3721 ctgcctctgg ccggcccgat gacaggctcc gcggcgaacc cgaaatgacg ggttacgccg
     3781 aagccccttg cgcctttggc cgccgcagat gttcatccag ccgcggcatg atctccacaa
     3841 aattgcaggg ccgcgtgcgg taatcaagct gggctgcgag aatgccgtcc cagccgtcgc
     3901 ggcaggcgcc cggcgatccc ggcaaacaga agataaacgt ggcccccgcg acacccgcgg
     3961 tggcgcggct ctggatcgtc gatgtgccga tcttggcgtg gctcagcatg tggaacgcga
     4021 tggagaaacc atccatacgc ttttcgaaca gcggctcgat cgcctccggc gtcacgtcgc
     4081 ggccggtgaa gccggtgccg ccggtggtga tgatggcgtc gatgccggga tcggcgatcc
     4141 atcttttgat gatgaggcgg atcgcatcga cctcgtcgac gatgatctcg cgcgccgcga
     4201 gcttgtgacc ggccgcggtg agccgctccg acagcgtcgt cccggatttg tcatcctcca
     4261 gcgagcgcgt gtcggaaacc gtcagcaccg cgatgttgag cggcacgaat tgcttggact
     4321 cgtcgatcga agccatgtgc gctttatccc tcatgtcccg gacgcggtgc agcgtccttc
     4381 acgctgctcc gcagagccgg gacccactcg cggatgacgt tacatgggcc ccggatcagc
     4441 agcgcaccgc cagcgcgctg cgcagcatcc ggggcgcgtg ctgctagatt acaacgttcc
     4501 cgcaccgaag gtattgcagg cctgcaccgt accctgctgg tagccggtca tgaaccattg
     4561 ttttcgttgc gcggcggaac catgggtgaa tgaatccggc accactcgcc ccgtcgcctg
     4621 ccgctgcagc gtatcgtcgc cgatcgcggt ggccgtggtc agcgccgcat cgatatcgcc
     4681 ggcttcaagg aagcccggac gcttcttctc ctcgcggttg acccagacgc ccgacaggca
     4741 atccgcctgc agctcgacct tcacctgtag cgcattggct tccgccttgc tgccggcctg
     4801 ctgctgcatc cgcgtcacgc gcggcaggat gccaagcagg ttttggatgt gatgtccggc
     4861 ttcatgggcg atgatatagg ccgcggtgaa tttgcacgca ttgcccgaac agccgtgaaa
     4921 ccgcgtctcg acctgacgga agaacccggt atcgagaaag atctgcttgt ccggcgggca
     4981 atagaacggc cccatcgccg cctgcgccat gccgcagcgc ccgccgttgg tggcgttgcg
     5041 aaacaatacg atgcgcggac cggaatagga ttgaccgctg gcctggaaga tttcgctcca
     5101 gcggtcgtcg atctcgccga ggacaccgga gatcatgcta cccatctcgt cggtcggcgc
     5161 gccggtcttg gcgggcgccg accggcgatc ggattgatag ctcggcgact gcccaccggt
     5221 gagaatttcc gcgccgccga tcaggatgcg cggatcaatg ccgaaggcgt agccgacgag
     5281 accgagcaca atcatggtgc cgatgcccag cccgccgccg cccatcggaa gaccgaagcc
     5341 gccaccaccg ccgccgccgc tgtcatcgcg acggtcatcg atatcgtcgc tgcggcggaa
     5401 gtcatcgtag cgcatggcgg tttttcctca ttccggtcgg ctggcagcac gctcgccgat
     5461 acccaacgcg ccaaccacgc aaaatttcca tttcataaat caataacccg cccggcaaaa
     5521 attacctagc aatcaatgcc taccaagtgc gccgcgataa cccggtgttt acctcggcac
     5581 gtcaaggtat ggccagtcgg ttgtttagtc ccgcgtcgcc gacagcgtcg cctgagtcag
     5641 agtatttgag tcatggttgt gtcgcgtcgc ggggcgtctg caagggcgcc ccgcactcaa
     5701 ttcgagtccg atccgagcac ccgtatcgtc gtcggcgatt gcgtcgccga gatgtcgaag
     5761 cttccggctg gctcggtcga tctggtgttc gcagatccgc cgtacaatct gcagctcaag
     5821 ggcgacctca agcgtcccga cgaatcccat gtcgacgccg tcaacaacga ctgggacaag
     5881 ttctcatcct tcgccgccta cgatgatttc acccgcgcct ggctgctcgc ctgccgccgc
     5941 atcatgaaac cgtcggcgac gctgtgggtg atcggctcct accacaacat tttccgcgtc
     6001 ggctcgatca tgcaggacct cggcttctgg gttctcaatg atatcgtctg gcgcaagacc
     6061 aatccgatgc caaatttccg cggccgccgc ttcaccaatg cgcatgagac catgatctgg
     6121 gccgcgcgcg acgagaaagc caagggctat accttcaact acgaggccct caaggccgcc
     6181 aacgaggacg tgcaggcgcg ttccgactgg ctgatcccgc tgtgcaccgg cgacgaacgc
     6241 ctcaagggcg aagatggcaa gaaagtccat ccgacccaga aacccgaagg tctgctggcg
     6301 cgcgtgctgc tgtcgtcgtc g
//
LOCUS       DATNOI010000469         7815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_553031,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000469 DATNOI010000000
VERSION     DATNOI010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553031"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VN968_18635"
     CDS             <1..207
                     /locus_tag="VN968_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31289.1"
                     /translation="DFFMIVCGPILLIERMARPEAALAVATGDAAAREWAWKRLETAT
                     ADPAHAANLGQMEPAMEPTARRRV"
     gene            536..2482
                     /gene="dxs"
                     /locus_tag="VN968_18640"
     CDS             536..2482
                     /gene="dxs"
                     /locus_tag="VN968_18640"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HXL31290.1"
                     /translation="MQNWNIAVTLFSKTPLLDTIRTPDDLRRLKVDQIRQVADELRQE
                     TIDAVSVTGGHFGAGLGVVELTTALHYVFDTPRDRLVWDVGHQAYPHKILTGRRDRIR
                     TLRTGGGLSGFTKRTESDYDPFGAAHSSTSISAALGMAVARDLTGGKNNVIAVIGDGA
                     MSAGMAYEAMNNAGAMNSRLIVILNDNDMSIAPPVGAMSAYLSRLYSGKTYRTLREAA
                     KQINKRLPKILANRANRVEEYSRGFMMDGGTLFEELGFYYVGPIDGHNLDHLLPVLKN
                     VRDMETGPILVHVVTQKGKGYGPAEASADKYHAVVKFDVATGAQAKAKPNAPSYQNVF
                     GQSLVKEAEKDEKIVGITAAMPSGTGIDIFNKAFPDRTFDVGIAEQHAVTFAAGLATE
                     GYKPFCAIYSTFLQRGYDQIVHDVAIQSLPVRFAIDRAGLVGADGATHAGSFDNAYLG
                     CLPNFVIMAASDEAELVHMVATQVAINDRPSAVRYPRGEGRGIEMPEFGVPLEIGKGR
                     IVREGTKIALLSFGTRLAECEKAADELAAHGLSTTIADARFMKPLDVDMVLQLARDHE
                     ILLTIEEGSVGGFGSHVMQTLSDHGMLDGGLRMRSMVLPDEFLDHDSPNAMYARAGLD
                     AKGIVAKVFDALGKDFKTGTVKLA"
     gene            2616..3188
                     /locus_tag="VN968_18645"
     CDS             2616..3188
                     /locus_tag="VN968_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HXL31291.1"
                     /translation="MKIYLAGPDVFLHDAVDIGRRKVELCRRHGLIGLYPLDNAIDPA
                     AQDVSLQIFRGNETMMMEADAIIANLTPFRGPGADPGTVYELGYMAGRGKLCLGYSNH
                     PSSYAERVREFTDVSSRDGRLVDALGLTVEDFGLTDNLMMIHALELHGCALVTPPQMP
                     ADIWHDLTAFETCVRLAAERQALPHPPVSN"
     gene            3212..3946
                     /locus_tag="VN968_18650"
     CDS             3212..3946
                     /locus_tag="VN968_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HXL31292.1"
                     /translation="MPPPRKRADLLLVERGLFESRARAQAAIEAGLVTANDKPVTKPS
                     EAIPADAALQAQPAHPFVSRGGVKLAGALQQYPIEIEDHVCLDVGASTGGFTEVLLAN
                     GASLVFSIDVGREQLHPALRGHPKIVSMEETDIRNFEGKRLPMRPDVGVIDVSFISLK
                     AVLPVALSLAAAPMHLLALIKPQFEAARKHSKRGIIRNAMVHQEICDDIAGFVASLGC
                     TGIQVFPSSITGGDGNIEFFIGARRG"
     gene            3939..5186
                     /locus_tag="VN968_18655"
     CDS             3939..5186
                     /locus_tag="VN968_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXL31293.1"
                     /translation="MVERLVIDHVGHRGDGVALAGGETVHVPYTLGGETVEVAPVPGH
                     HPDRRRLLQVERASPDRITPFCPHFATCGGCAIQHWDSERYRAWKREIVVETLAQAKL
                     ACEVGPLIDAHGLGRRRITLHARIGMHDVLKVGFAAANSHDIIPVDRCPILDPGLSGA
                     IDAGWAVAEPLKATEKPLDIQITSTDNGLDIDVRGSGPLPAAMIATLSRVAEEHRLAR
                     LTRHGELVLMRTPPTIPIGTAQVTLPPGSFLQATVAGEEALAALVSGHCRRAKHVADL
                     FCGVGPFALRLAAKSRISAFDSDAGAVAALQKAATATSGLKPVKAEARDLFRRPLMPQ
                     ELRDFDAIVFDPPRQGAQAQVLQLAASKIPVVVAVSCNVATFARDAKILIDGGYKIEG
                     VTPVDQFRHTPHVELVARFVPRR"
     gene            5356..5958
                     /locus_tag="VN968_18660"
                     /pseudo
     CDS             5356..5958
                     /locus_tag="VN968_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(5978..6856)
                     /locus_tag="VN968_18665"
     CDS             complement(5978..6856)
                     /locus_tag="VN968_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HXL31294.1"
                     /translation="MRWCVSIGVVLVAGAMAGGDVAGIAAPSTRNQLTDQLGPQPGLE
                     LAAPKDSTPSVDIELILAVDVSYSMDMDELAIQREGYAQAIVSKEFLQALKTGPTGKI
                     SITYFEWAASSDQKIIIPWRVIDGPETADAVANEIMKTPIRRASRTSISGAINFAMPL
                     FDEDPYRGLRRVIDISGDGPNNNGTPVTGARDAALEKGIIINGLPIMVKEPSYSTMDI
                     DNLDFYYEDCVIGGPGSFVVSIKDREKFKEAIRTKLLLEVAGRTPERRIVPVADKEPR
                     VSCLIGEKIWQDRWGR"
     gene            7138..7443
                     /gene="clpS"
                     /locus_tag="VN968_18670"
     CDS             7138..7443
                     /gene="clpS"
                     /locus_tag="VN968_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HXL31295.1"
                     /translation="MIHSVTKPKTKVKTKTERPRLHKVILVNDDFTPREFVVTVLKAE
                     FRMTEDQAHKVMITAHRRGVCVVAVFTKDVAETKATRATDAGRAKGYPLLFTTEPEE"
     gene            complement(7483..>7815)
                     /locus_tag="VN968_18675"
     CDS             complement(7483..>7815)
                     /locus_tag="VN968_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31296.1"
                     /translation="VDFTKFDNRTLAIMALNTDASFSTEEAGAAKAELTQRSRSALVK
                     AFDPKTGGGAMASSQALLQQYSSMSAEEKKVLGFTDDYANKIAQNYRTMSSLQNSLGS
                     GAGLTSYF"
BASE COUNT         1436 a         2479 c         2409 g         1491 t
ORIGIN      
        1 gattttttca tgatcgtctg cgggccgatc ctgctgatcg aacgcatggc tcgtcccgag
       61 gcggcgctgg cagtggcgac cggcgacgcc gccgcccgcg aatgggcctg gaaaaggctg
      121 gaaaccgcga ccgccgatcc ggcacatgcc gccaatttgg gccagatgga acccgcgatg
      181 gaaccaacgg cgcggcgcag ggtttgaaat ccggcgttct gccaccctga tccatcgagc
      241 gctgcgggtt ccagcggatt ggctttgacc gcagctttgg tgtagaggtt tgacggctat
      301 ggggtgttga aagagacctg tcaccggtct cgtacggttg taagcgcttg taatagcttg
      361 ttaaatttct tgagaagggt gacgccgcca agagtgatac gtatcacact gaacgatctg
      421 gagcgcatct aggctttcaa ctccggctac agctgctcaa gatggcatct ggcgtctggg
      481 ttttcgctgc gctgatattg cgcaacccat ctcacgtcgg acgccttgtc ccgacatgca
      541 aaattggaat atcgccgtga cactattcag taaaacgccg cttctcgaca caatccgcac
      601 ccccgatgat ctgcgccggc tcaaagtgga ccaaatccgg caagtcgccg atgaactgcg
      661 ccaggagacc atcgatgccg tctcggtcac gggcgggcat ttcggcgccg gcctcggcgt
      721 cgtcgaattg accacggcgt tgcactacgt gttcgacacc ccgcgcgacc gtttggtctg
      781 ggacgtcggc catcaggcct atccgcacaa gatcctgacc ggccgccgcg atcgcattcg
      841 caccctgcgc accggcggcg ggctgtccgg cttcaccaag cggaccgaaa gcgattacga
      901 tccgttcggt gcggcgcact cctccacctc gatctccgca gcgctcggca tggccgtggc
      961 gcgcgacctc accggcggca agaataatgt tatcgccgtg atcggtgacg gtgcgatgtc
     1021 ggccggcatg gcctatgaag ccatgaacaa tgccggcgcg atgaactccc gcctgatcgt
     1081 cattctcaac gacaacgaca tgtcgatcgc gcctccggtc ggagcgatgt cggcttacct
     1141 gtcgcggctt tactccggca agacctaccg cacgctgcgc gaggcggcaa agcagatcaa
     1201 caagcgtctg ccgaaaatcc tcgccaaccg cgccaaccgt gtcgaagaat attcccgcgg
     1261 cttcatgatg gacggcggca cgctgttcga ggaactcggc ttctattatg tcggtccgat
     1321 cgacggccac aacctcgacc atttgctgcc ggtcttgaag aacgtccgcg acatggagac
     1381 cggcccgatc ctggttcatg tcgtcacgca aaagggtaag ggctacggcc cggcggaagc
     1441 gtccgccgac aagtatcatg cggtggtcaa gttcgacgtt gccaccggcg cgcaggccaa
     1501 agccaagccg aatgccccct cgtatcagaa cgtgtttggc caaagcctcg tcaaggaagc
     1561 cgaaaaggac gagaagatcg tcggcatcac cgcggcgatg ccgtcgggaa ccgggatcga
     1621 cattttcaac aaggcgttcc ccgaccgcac cttcgacgtc ggcatcgccg agcagcatgc
     1681 ggtgaccttt gccgcgggtc ttgcgaccga aggctacaag ccgttctgcg cgatctattc
     1741 gaccttcctg cagcgcggct atgaccagat cgtccatgac gtcgcgatcc agagcctgcc
     1801 ggtgcgtttc gcgatcgatc gtgccgggct tgtcggtgct gacggcgcca cccatgccgg
     1861 ctcgttcgac aatgcctatc tcggctgcct gcccaatttc gtcatcatgg cggcctccga
     1921 tgaggccgaa ctggtgcaca tggttgcgac ccaggtcgcg atcaacgacc gtccgagcgc
     1981 ggtccgctac ccgcgcggcg aaggccgcgg catcgagatg cccgaattcg gcgtaccgct
     2041 tgagatcgga aaagggcgca tcgttcgcga gggcaccaag atcgcgttgc tgtcgttcgg
     2101 cacccgtctt gccgaatgcg agaaggccgc ggacgaactg gcggcgcatg gactgtccac
     2161 caccatcgcc gacgcacgct tcatgaagcc gctcgacgtc gacatggtgc tgcagcttgc
     2221 gcgtgaccac gaaatcctcc tcaccatcga ggaaggttcg gtcggcggct tcggctccca
     2281 tgtcatgcag acgctgtcgg atcacggcat gctcgacggc ggtttgagga tgcggtcgat
     2341 ggtgctgccc gacgagttcc ttgaccacga ctcgccgaac gcgatgtacg cccgcgccgg
     2401 tctcgacgcc aagggcatcg tcgccaaggt gttcgatgcg ctgggcaagg atttcaagac
     2461 cgggacggtg aagctcgcct gacaaaccgg ctgcgccggc ggccctcgtc tgaaccggcg
     2521 ccagccgctg cttggcgggt ggcgctttcc tgccccattt tgttagcttg ccgtcgcaag
     2581 accttcaatc gtgcgcagaa tccgcgcaat taaagatgaa gatttacctg gctggccccg
     2641 acgttttcct gcacgacgcc gtcgatatcg ggcggcgcaa ggttgaattg tgcaggcgtc
     2701 atggactgat cggactgtat ccgctggaca atgcgatcga tcccgcggct caggacgtgt
     2761 ctttgcagat ctttcgcggc aacgaaacca tgatgatgga ggccgatgcc attatcgcca
     2821 acctcacgcc gtttcgcggc ccgggcgcgg accccgggac cgtctacgaa ctcggctata
     2881 tggccggacg cggcaagctc tgtctcggct actccaacca tccctcgtcg tatgccgagc
     2941 gtgtccgtga attcactgac gtgagttcac gcgatggacg cctggtcgat gcgctgggat
     3001 tgactgtgga ggatttcggg ttgaccgata atctgatgat gattcatgcg ctcgagctgc
     3061 atggctgtgc gctggtgacg cctccgcaaa tgccggccga catctggcac gatttgaccg
     3121 cgttcgagac gtgcgtgcgc ttggcggccg agcggcaggc cttgccgcac ccgcctgtat
     3181 cgaattgaac ttgccccgcc acgaggcgaa catgccccct ccccgcaaac gcgcggacct
     3241 gttgctggtc gagcgcggtc tgttcgaaag ccgggcccgc gcccaggccg cgatcgaggc
     3301 gggcctggtc accgccaatg acaagccggt cacaaaacca tccgaggcca ttcccgccga
     3361 tgccgcgctg caggcgcagc ctgcgcaccc ctttgtctcg cggggcggcg tcaaactggc
     3421 gggggcgttg cagcagtatc cgatcgagat cgaggatcac gtctgtctcg acgtcggcgc
     3481 atcgaccggc ggcttcaccg aggtattgct cgccaacggc gccagcctgg tgttttcgat
     3541 cgatgtcggg cgcgagcaac tgcatcccgc gctgcgcggc catcccaaaa tcgtttccat
     3601 ggaggaaacc gatatccgga acttcgaagg caagcgtctg ccgatgcggc cggacgtcgg
     3661 cgtcatcgac gtcagtttca tttcgttgaa agccgtgctg ccggtggcgc tgtcgctggc
     3721 ggcagcgccg atgcatctgc tggcattgat aaagccgcaa ttcgaggcgg cgcgaaagca
     3781 ttccaagcgc ggcatcattc gcaatgcgat ggtgcaccag gaaatctgcg acgacatcgc
     3841 aggcttcgtc gcctcgctcg gatgcaccgg catccaggta tttccgtcct cgatcacagg
     3901 tggcgacggc aacatcgaat tcttcattgg cgcgcgccgt ggttgagcgt ctcgtcatcg
     3961 atcacgtcgg tcatcgcggc gacggcgtcg cgcttgccgg cggcgagacc gtgcatgtgc
     4021 cctacacgct cggcggcgag accgtcgagg ttgcgcccgt gccgggtcat catcccgatc
     4081 gccggcgtct gctgcaggtc gagcgcgcca gcccggatcg gatcacgcca ttctgcccgc
     4141 attttgcaac ttgcggggga tgcgcgatcc agcactggga cagcgaacgc taccgcgcct
     4201 ggaagcgcga gatcgtcgtc gagacgctgg cacaagccaa actcgcctgt gaagtgggcc
     4261 cgctgatcga tgcccatggc ctcgggcgcc ggcggattac cctgcatgcc cggatcggca
     4321 tgcacgatgt gctcaaggta ggattcgccg ccgcaaattc gcacgacatc attcccgtgg
     4381 atcgctgtcc cattctcgac cccggcttga gcggcgcgat cgacgcgggg tgggcggtgg
     4441 ccgagccgct gaaagcaacc gaaaagccgc tcgatatcca gatcacctcg acggacaacg
     4501 gcctcgatat cgatgtgcgc ggctcggggc cgctgccggc ggcaatgatc gcaacgctgt
     4561 cgcgcgttgc cgaagagcat cgcctggcgc ggctgacgcg ccatggcgaa ctggtgctga
     4621 tgcggacacc gccgacgatt ccgatcggca ccgcgcaggt aaccctgccg ccgggttcgt
     4681 tcctgcaggc caccgtcgcc ggcgaggaag cgctggcggc gctggtatcg gggcattgca
     4741 ggcgcgccaa acacgtcgcc gacctgtttt gcggggtcgg accgttcgcg ctgcgtcttg
     4801 cggcgaaatc gcggatttcg gcgttcgaca gcgatgccgg cgcagtggcc gcgctgcaga
     4861 aggcggcgac ggcgacgtcc gggctgaagc cggtcaaggc cgaggcccgc gacctgttcc
     4921 ggcgtccgct gatgccgcag gagctgcgcg actttgacgc catcgtgttc gatccgccgc
     4981 gccagggcgc gcaggcgcaa gtcctccagc ttgcggccag caagattccc gtggtggtcg
     5041 cggtgtcatg caatgtcgcg acgtttgccc gcgacgccaa aatcctgatc gatggcggct
     5101 acaagatcga gggcgtgacg ccggtcgatc agttccgcca caccccgcat gtcgagctgg
     5161 tggcgcggtt tgtgccgaga cgttgaacct cccttgcagc cggatgacat acctgagtga
     5221 aggactgaca caggcgatct gtctctggtc gagcgaccgc cttgccggtc gtccgcacga
     5281 cgcggaccaa gccacctcat tgaagatgtc tgacatccaa aggaggccat gcgttgcttc
     5341 cgtgcatcct accagcatca cctggtgcag gagtggctgc ccgcgctgga tggtgtggtc
     5401 gagaaactca agcgcggcgc caaagtcgcc gatgttggct gtggccatgg catctcgacc
     5461 agactaatgg ccaaggcatt tccaaattcc cagttcttcg gcttcgatgc gcatccgggc
     5521 tcaatcgatg ccgcgcgcaa ggcggcaaag gaagaagggt tcggcgaacg tctgtcgttt
     5581 atgactgcga ccgccaagac atttccgtcc aatggatatg accttgtctg cttctttgat
     5641 tgcctgcacg acatgggcga cccggtcggc gctttgaagc atgtcgtcac cacgatggcg
     5701 gaagatggca cttgcatgct ggtggagccg ttcgccaacg acaagctcga acaaaatctc
     5761 aatccggtcg gaagtctttt ctatgctgcg tccgccatga tctgcacgcc agcctcgatg
     5821 gcgcaggagg tcggtcttgc gctcggcgcg caggccggtg aagcgcgact tcgcgacgtg
     5881 gccaataaag gcggcctatc gcgattccga cgcgcagcag aaacaccgtt caatctgatt
     5941 ctggaggcgc gggcctaacc agggccgtcg gaccggttta ccttccccaa cgatcctgcc
     6001 agatcttctc gccgatcagg caggagacgc gcggttcctt gtcggcgacc ggcacgatcc
     6061 ggcgctcggg cgtccggccc gcgacttcga gcagcagctt ggtccggatc gcctccttga
     6121 acttttcgcg gtccttgatc gagaccacga aggagcccgg gccgccgatg acgcaatcct
     6181 cgtagtagaa atcgagatta tcgatgtcca tcgtcgaata ggacggttcc ttcaccatga
     6241 tcggcaggcc gttgatgatg atgcccttct ccagcgccgc gtcgcgcgcg ccggtgaccg
     6301 gcgtgccgtt attgttgggg ccgtcgccgg aaatatcgat gacgcgccgc aagccgcgat
     6361 acgggtcctc gtcgaacagc ggcatcgcga aattgatcgc gccggagatc gaggtgcgcg
     6421 acgcccttcg gatcggcgtt ttcatgatct cgttggcgac cgcgtccgcc gtttccgggc
     6481 cgtcaatcac ccgccaggga atgatgatct tctggtcgct cgaggccgcc cactcgaaat
     6541 aggttatcga aatcttgccg gtcggaccgg tcttcagcgc ctgcaggaat tccttcgaga
     6601 caatggcctg ggcgtagccc tcgcgctgga tcgcgagttc gtccatgtcc atcgaatagg
     6661 agacatcgac ggccaggatc agttcgatat cgaccgacgg cgtagagtcc ttcggcgcgg
     6721 ccaattcaag gccgggttgc gggccaagct gatcggtcag ctgattcctg gtgctcggcg
     6781 cggcgatacc cgcgacgtca cctcccgcca tcgcccccgc gacaagcacc accccgatcg
     6841 agacacacca gcgcatagcg gccctcacgt cgtatgacgc gatggtgaca cgcaaaccag
     6901 acttcgaaaa gcgcgaacat ggattgtgct tcacattcaa gttagcccgg ttgccttaat
     6961 ttcacccgcc ttccgtttcg ccgatcgcgt tacctcgaat gatccgacag gcggaccgac
     7021 gacacgcgca gcttgccgcc actgcaatga agtcatcgcg gaactgcagg tttcgcgacg
     7081 gatcgagacg ttgtaaactg gtcgcgacgg cccgtaacgt caggaggcgc cgcctgaatg
     7141 atccatagcg tcaccaagcc gaaaaccaag gtcaagacca agaccgaacg gccgcgcttg
     7201 cacaaggtca tcctggtcaa cgacgacttc acgccccgcg aatttgtcgt gaccgtcctg
     7261 aaagcggaat tccgcatgac cgaggaccag gcccacaagg tcatgatcac cgcgcaccgg
     7321 cgcggcgtat gcgtggtcgc ggtcttcacc aaggacgtgg ccgagaccaa ggcgacccgc
     7381 gccaccgacg ccggccgcgc caagggttat ccgctgctgt tcaccaccga gccggaggaa
     7441 tagcgcgcta tttccggccc tccaccgtcc gcgcctcaac ggttaaaagt atgacgtcaa
     7501 cccggcgccg gagccgaggc tgttctgtag cgacgacatc gtgcggtaat tctgcgcgat
     7561 cttgttggcg tagtcgtcgg taaagccgag gactttcttc tcctcggcgc tcatgctcga
     7621 atattgctgc agcagtgcct gggagcttgc catggcgccg ccgcctgttt ttggatcgaa
     7681 agctttcacc agggcgctgc ggctgcgctg agtgagttcg gccttggcgg cgcctgcttc
     7741 ctcggtcgag aacgatgcgt cggtgttcag ggccatgatc gccagggttc tgttgtcgaa
     7801 tttcgtgaaa tccac
//
LOCUS       DATNOI010000470         2213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2221786,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000470 DATNOI010000000
VERSION     DATNOI010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221786"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VN968_18680"
     CDS             <1..566
                     /locus_tag="VN968_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31297.1"
                     /translation="WGSFHRHPEETTMGKVIKIRDAQIRTVSIEIKSMTIDDRQVTLA
                     LFRQLIEEDLISRAGTLNGLPWGFVNYHPGKCADSKLEHWHIVWQKGDELRRATELQE
                     PRFGPLCSDLIDQWANVSVCVAFEAAPGVRLNEHVEADGPTVFAHACRPGVRGGAARG
                     GRGLEPMTEGKNRIMLYGAGADRPGRL"
     gene            complement(563..1528)
                     /locus_tag="VN968_18685"
     CDS             complement(563..1528)
                     /locus_tag="VN968_18685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31298.1"
                     /translation="MNSIYKLVIALLARVVLSAFVMWPAMAGAADNPDWAYPITPPPK
                     PLDNTVMRQMPGSTKQYTQAQIDDPFNPPDWYPDEHPPMPEIVARGGPKPDGRACAQC
                     HLTSGDGHPESSSLAGLPAAYIIWQMQAFKDGDRKGIRSGLMIAMAKVLSDAEVKAAA
                     VYFAALKPAAGYNKVVETETVAKSYVGAGGMRFAMPNGGSEPIGQRIIVLPQDEARAD
                     ARDPKSGFIDYVPVGSIVKGAALMAGGEGKTVDCTICHGPTLRGLGEVPGIAGRPATY
                     TFRQLNDMKTGNRKGPWVELMKQVVEKLTDEDMISLAAFLGSLDP"
     gene            1699..>2213
                     /locus_tag="VN968_18690"
     CDS             1699..>2213
                     /locus_tag="VN968_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31299.1"
                     /translation="MTFGIGRREFITLLGSAAAWPLVARAQQAMPVVGFLGGTSPDTF
                     TDRLRAFRQGLKDTGYVEGENVAIEYRWAENQIDRLPALAADLVRRQVAVIAATGGIT
                     SAFAAKAATTMIPIVFGAGEDPVRLGLVASLARPGGNLTGVNFLSAELTAKRLELVRE
                     LVPAATRISVLV"
BASE COUNT          391 a          664 c          728 g          430 t
ORIGIN      
        1 cctggggcag ttttcatagg catccagagg agacgacgat gggtaaggta atcaaaatcc
       61 gcgatgccca aatcaggacg gtctcgatcg agatcaagtc gatgactatc gacgaccggc
      121 aggtgacgct ggcgctcttc aggcagctga ttgaggaaga cctgattagc cgcgctggca
      181 cgctcaatgg tttgccgtgg ggattcgtga actaccatcc cggcaagtgc gccgattcaa
      241 agctcgagca ctggcatatc gtctggcaga agggcgacga gctaaggcgg gctacggaat
      301 tgcaagagcc caggtttggg ccgctttgct ctgacctgat cgatcaatgg gcgaatgttt
      361 cagtttgtgt cgcgttcgag gccgcgcccg gcgtgcggct caacgagcac gtcgaggccg
      421 acgggccgac cgtgttcgcc cacgcctgca gacccggcgt gcgaggcggt gcggcgcgag
      481 gcggaagagg attggagccg atgaccgagg gcaagaaccg catcatgctt tatggcgccg
      541 gcgcagatcg cccgggcagg ctttaggggt cgagcgagcc caggaaggcg gcaagcgaga
      601 tcatgtcttc gtcggtgagc ttctcgacca cctgcttcat cagctcgacc catgggccct
      661 tgcggttgcc ggtcttcatg tcgttgagct ggcggaacgt gtaggtcgct ggccggccgg
      721 cgatgcctgg gacttcgccg agccccctga gcgtgggccc gtggcagatg gtgcagtcga
      781 cggtcttgcc ttcgccgccc gccatcagcg ctgcgccttt gacgatgctg ccgaccggaa
      841 cgtaatcgat aaagccggat ttgggatcgc gggcatcggc cctcgcctcg tcctgcggca
      901 atacgatgat ccgttggccg atcggctcgc taccgccatt cggcatggcg aagcgcatgc
      961 cgccggctcc aacatagctc ttcgccacag tttcggtttc gaccaccttg ttgtaacccg
     1021 ccgccggctt cagcgcggcg aaataaacgg cggcggcttt cacctccgca tctgacagaa
     1081 ccttcgccat tgcgatcatg aggcctgacc ggatgccctt gcggtccccg tccttgaagg
     1141 cctgcatctg ccaaataatg tatgcggctg gcagcccagc aaggctggag gactccgggt
     1201 ggccgtcgcc tgaggtcaga tgacactgcg cgcaggcacg gccatccggc ttcggcccgc
     1261 cacgcgccac aatctccggc atcggcggat gttcgtctgg gtaccaatcg ggtgggttga
     1321 acggatcgtc gatctgcgcc tgggtgtatt gcttggtgct gcccggcatc tgccgcatga
     1381 ccgtgttgtc cagcggcttg ggcggcggcg tgatcggata ggcccaatcc ggattgtcgg
     1441 cggcgccggc catcgctggc cacatcacga atgccgacag aacaacgcgc gcaagcaggg
     1501 ctatcactag tttgtagatt gagttcattg tgatttcctt tgctgatcga aatgataatc
     1561 gcgagtcgtc gcaacgaccg tgccggcgca cctcggtgtc aatcaaatcg attatgcgct
     1621 tgtggtgccg gatgtgaagc cggacacgga ccgatgaggg tgctatcctt cagtcgccct
     1681 tccgaccgga gggatcagat gacatttggc atcgggcgcc gcgagttcat cacgctgctg
     1741 ggcagcgcgg cggcgtggcc gctcgtggcg cgcgcgcagc aggcgatgcc ggtggtcgga
     1801 ttcctcggcg gcacatcgcc tgacacgttc acggaccgtc tgcgcgcatt ccgccagggc
     1861 ctgaaggaca ccggctatgt cgaaggcgag aacgtggcga tcgaataccg ctgggcagag
     1921 aatcaaatcg atcggctgcc ggcgctggcg gccgacttgg ttcgccggca ggttgctgtg
     1981 atcgccgcga ccggaggcat cacttcggca ttcgcggcca aggcggcaac cacgatgatc
     2041 ccgatcgtct tcggcgccgg tgaagacccg gtgaggttgg gtcttgtcgc cagcctcgcc
     2101 cggccaggcg gcaacctgac aggcgtcaat tttctcagtg ctgagctgac ggcaaagcgg
     2161 ctggaactcg tgcgtgagct ggtacccgca gcgactcgca tttcagtgct cgt
//
LOCUS       DATNOI010000471         4300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1170701,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000471 DATNOI010000000
VERSION     DATNOI010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170701"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="VN968_18695"
     CDS             <1..134
                     /locus_tag="VN968_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXL31300.1"
                     /translation="AKAGTIPGVRTPIIIGGKPMAAERPSPRLGEHTAEVLREVGEG"
     gene            424..1455
                     /gene="dctP"
                     /locus_tag="VN968_18700"
     CDS             424..1455
                     /gene="dctP"
                     /locus_tag="VN968_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXL31301.1"
                     /translation="MTISRRCVLAGALSAPAIVASTRLGSAAPAQTLKISHQFPGGTI
                     DEGDFRDRLCRKFAAELEKRTNGALATQVYPNSSLMKTVAQFSAVRKGVLDLSLYPIS
                     YGGGEFAELNIGLMPGLVNSYKQGAAWKQAEVGQKFNQFLAGKGVVMVSWIWQAGGVV
                     SRSTPIVNPQDVKGLKVRGGSREMDMVLQAAGATTLTLPSNELYAAMQTGACDAAITS
                     STSLISFRLEELAKNLTTGRGKSYWFMLEPLIMSKQVFDALPKNQQDVIMSLGVEMEQ
                     FGTKEAMADDERISEVYAKKGAKVFDLDEATVDKWRAIARDTAWKDYAQKTPLSAELL
                     KLAEGIQLS"
     gene            1588..1986
                     /locus_tag="VN968_18705"
                     /pseudo
     CDS             1588..1986
                     /locus_tag="VN968_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045348.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
     gene            2016..3365
                     /locus_tag="VN968_18710"
     CDS             2016..3365
                     /locus_tag="VN968_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXL31302.1"
                     /translation="MSLLSIGLLYGGATLFVMFLGMPIAFALGAVATLFMIIFMPRGS
                     VDTIAQNVYEEMASITLLSIPLFILKGAAIGRSPAGRDLYGALHAWMHWIPGGLGIAN
                     VFACALFAAMAGSSPATCSAIGSAGIPEMRKHGYSPGFASGIIAAGGTLGILLPPSIT
                     MILYAVAAEQSLGRLFLAAIGPALLLVALFAGYAMWKFRREYRAALAQYKLTGAQSEL
                     LRVDTLTVYDKFALLPRVLPFVILLTGVMIALYGGYATPSETAGLGAVLALALIASIY
                     SVWTPRDLEPILIATLRESTMLMMIIGMSLLYSYVMSYLHISQSTAQWIVSLELSRWV
                     LLAAILLMVVVLGFFLPPVSIILMTAPIILPPLKAAGFDLIWFGVIMTIVMEMGLIHP
                     PVGLNIFVIKNIAPDIPLRDVVWGVMPFVGLMMLAVVVLCIFPEIATWLADAIMGAK"
     gene            complement(3384..3854)
                     /locus_tag="VN968_18715"
     CDS             complement(3384..3854)
                     /locus_tag="VN968_18715"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HXL31303.1"
                     /translation="MQRIVIFLLGLVLGGGLMASVPLGKAHDDLTGALASPENHPLLL
                     HMTTGDSWRGATGLEFARAMLKVGHPVAVFLNLDAVRLALRTGEQEKKASMPQVPREI
                     VADLIRDGAIVLICQPCLSEFGFKLDDIVPGVQLGAPGYLENFVFADNVRTFTW"
     gene            4025..>4300
                     /locus_tag="VN968_18720"
     CDS             4025..>4300
                     /locus_tag="VN968_18720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL31304.1"
                     /translation="MIRLLSIALASAVIAISAIAEAGEDGWPERPLRLVVPFPAGSST
                     DIIARIVAQKLSHRLGQQVVIENRAGASGNIGADAVAKAAPDGYTIGI"
BASE COUNT          764 a         1318 c         1345 g          873 t
ORIGIN      
        1 ccgccaaggc cggaacgata ccgggcgtac gcacgccgat catcatcggc ggcaaaccca
       61 tggcggcgga acggccctcc ccgcggctcg gcgaacacac ggcggaagtt ttgcgcgagg
      121 tcggcgaggg gtgaagcgcg cgaccgaccg tggctatttg acgcgcgccg ttcgtcccag
      181 cggcatcgtc gatgtggagc agcgctcggg ctgacgcgtg agtgcgcgtt cgtgttgacg
      241 ttgcaagcat taagccctga tgggagatgt gaattggttc gcgcgtcgat ccagcaacga
      301 tcggttatat tgcacttatt ttcatcggac ttatattgag ccggccaaat gatttcggcc
      361 taaattcaac cgcggtcgcc cggcaaggcc tcggacagag gcgaccggat ggggaggatg
      421 aacatgacca tcagccgacg ctgtgttctt gccggtgcgt tgagcgcgcc cgcgatcgtc
      481 gcttcgacac gattgggctc ggctgcgccg gcccagactt tgaaaatctc tcaccagttc
      541 cccggcggca cgatcgacga aggtgatttc cgtgatcggc tatgccgcaa gttcgccgcc
      601 gagctcgaga agcggacgaa tggcgcgctc gccacacagg tctatcccaa ctcgtcgctg
      661 atgaagaccg tggcgcaatt ttccgcggtg cgcaaaggcg tgctcgattt gagcctttat
      721 ccgatctcct atggcggcgg ggagttcgcc gagctcaaca tcggcctgat gcccggattg
      781 gtgaattcct acaaacaagg cgccgcctgg aagcaagccg aggtcggcca gaaattcaac
      841 cagttcctcg ctggcaaggg cgtcgttatg gtgtcctgga tctggcaagc cggcggggtg
      901 gtgagccgca gcacgccaat cgtcaatcca caggacgtca agggattgaa ggtgcgcggc
      961 ggcagtcgcg agatggacat ggtgttgcag gccgccggtg ctacgaccct gaccttgccg
     1021 tcgaacgaac tctacgccgc gatgcagacc ggtgcttgcg acgccgccat cacatcctcg
     1081 accagcctga tctcgttccg gctggaagaa cttgccaaga atctcaccac cgggcgcggc
     1141 aagtcctact ggttcatgct ggagccgttg atcatgtcga agcaagtctt cgatgcgctg
     1201 ccaaagaatc agcaggatgt aatcatgtcg ctcggcgttg aaatggagca gttcggcacc
     1261 aaggaagcca tggccgacga cgagaggatc tccgaggtct acgccaagaa gggtgccaag
     1321 gtgttcgatc tcgacgaagc aaccgtcgat aaatggcgcg ccattgcccg tgacaccgcg
     1381 tggaaggact acgcccagaa gacgccgttg tcggcggaac tcctcaagct cgccgagggc
     1441 attcagctct cctgacctcg gcgaggctct gcgatggctc acggcctcga cgatgtcgag
     1501 accgggcagg cagcgacgct cccccggggc gccgctaacc gcatccttgc attcgtcaat
     1561 gacctgatgg tcaaggcatc gtcggccgca ctggtcgcgg ccggttttgt gctcacttac
     1621 agcgtcatcg tccgctattt cctgaaaatc tccaccgact ggcaagatga aacgtcggtc
     1681 ttcctgatcg tcggcgcggt cttcatgtcg tccgccgcgg tgcaggcgct gcgcggtcac
     1741 gtggcgatcg agacaattgt cgggtttttg ccggcaggcg tgaacagggc tcgacagatt
     1801 ctggtcgatg tcgggagtct ggcattctgc gccttcttcg cgtggaaatc ctgggccctt
     1861 ctgcatgaag cgtgggtgga cgactaccac tccggatcga cgtggggccc gccgttatgg
     1921 ataccttact cgctcatgac gatcggcatg gcgctgctat ccatccagat cctgctccag
     1981 gtcatgggtg agctcaggcg ccggagtcgc gcccgatgag cctgttgtcg atcggcttgc
     2041 tttatggcgg cgcgaccctg ttcgtgatgt ttctcggcat gccgatcgca ttcgcgctcg
     2101 gtgcagtggc gacattgttc atgatcatct tcatgccgag gggttccgtc gacacgattg
     2161 cccagaacgt ttacgaggaa atggcctcca tcaccttgct gtcgattccg ctgttcatat
     2221 tgaaaggcgc ggccatcgga cgctcgccgg cgggtcgaga tctttacggt gcgttgcacg
     2281 cttggatgca ttggattcct ggcgggctcg ggatcgccaa cgtgttcgcc tgcgcattgt
     2341 tcgcggcaat ggccggctcc tcgccggcca cttgctcggc gatcggctcc gccggcatcc
     2401 cggagatgcg aaagcacggc tactcgcccg gatttgcctc cgggatcatc gcagcaggcg
     2461 gcacgctcgg catcctgctg ccgccatcaa tcaccatgat cctctatgcg gtcgccgccg
     2521 agcagtcgct cgggcggctt ttcctcgccg ccatcgggcc agcgctcttg ctggtcgcgc
     2581 tcttcgccgg ctacgcgatg tggaagttcc ggcgcgaata ccgtgcggcg ctggctcagt
     2641 acaagctcac cggcgcccaa tcggagctgc tgcgggtcga cacgctcacg gtgtacgaca
     2701 aattcgcgct gctgccccgg gtcctgccct tcgtcatcct gctgaccggc gtgatgatcg
     2761 cgctctacgg cggctacgct acgccgtcgg aaacggccgg attgggcgcc gtgctcgctc
     2821 tcgcgctgat cgcatcgatc tacagcgtgt ggacgccgcg cgatttggag ccaatcctca
     2881 tcgccacgct acgcgagtcc accatgctca tgatgatcat cggcatgtcg ctgctctatt
     2941 cgtacgtgat gagctatctg cacatcagcc aatcgacggc acagtggatc gtttcgttgg
     3001 agctctcgcg ctgggtgctg ctggcggcga tcctgctgat ggttgtcgtg ctcggtttct
     3061 tccttccacc ggtttccatt atcctgatga cggcaccgat aatcctgcca ccgctcaagg
     3121 cggccggctt cgacctcatc tggttcggcg tgatcatgac cattgtcatg gagatggggc
     3181 tgattcatcc gccggtaggc ctgaacattt tcgtcatcaa gaatattgcc cccgatatcc
     3241 ctctgcgcga cgtggtatgg ggcgtgatgc cgtttgtcgg actgatgatg ctcgccgtcg
     3301 tcgtgctgtg catcttcccg gagatcgcga cctggttggc ggacgcgatc atgggcgcaa
     3361 aataacgtga ttctcgggcg ccactaccac gtgaacgtgc gcacattgtc ggcgaagacg
     3421 aaattctcga gatagcccgg cgcgccgagc tgcacgcccg gcacgatgtc gtcgagcttg
     3481 aagccgaatt cagagagaca cggttggcag atcagcacga tggcgccatc gcggatcaag
     3541 tcggcgacga tttcgcgcgg gacctgcggc atcgaggcct tcttctcctg ctcgcctgta
     3601 cggagcgcaa gccggaccgc gtcgagattg agaaacacgg ccacggggtg gccgaccttc
     3661 aacatcgcac gggcaaattc gagcccggtc gcaccgcgcc aggaatcccc ggtggtcatg
     3721 tggagcaaga gcggatggtt ttccgggctg gcgagagcgc cggtgaggtc atcgtgcgct
     3781 tttcccagcg ggaccgacgc catcagtccc ccaccgagga caagaccgag cagaaaaatg
     3841 acgatccgtt gcatgggtgt gacctcgctc caacacgtcg gctccgtagg ctagatgcta
     3901 ctctctcctg aatcggctgc agggtccatg gcgggagtct tgacctgatc gcgcggacat
     3961 cgaagcattg cacgcaacac gaggtgtccg cgctctgccg cggggcgtgc aggcgaggtt
     4021 tgccatgatt cggctgctat cgattgcgtt ggcatcggcg gtaatcgcga tctctgccat
     4081 tgccgaggcc ggcgaggacg gatggccgga gcggccgctc cgcttggtcg taccctttcc
     4141 agccggcagc agcacagaca tcattgcgcg catcgtggcg cagaagctga gccaccggct
     4201 cggccagcag gtcgtgatcg agaaccgtgc cggcgcgagc ggcaatatcg gcgccgatgc
     4261 ggtcgccaag gccgcgcccg acggctacac gatcggcatt
//
LOCUS       DATNOI010000472        29343 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1095140,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000472 DATNOI010000000
VERSION     DATNOI010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 29343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29343
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095140"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            612..1997
                     /locus_tag="VN968_18725"
     CDS             612..1997
                     /locus_tag="VN968_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31305.1"
                     /translation="MTPKAIRALSLTVAAIGLLYAAAPAIAQEKKITVWWGKGFYKSE
                     DDALLEAIKKFEAKTGIKVELSQYAIQDMIPKTVAALDSGTVPDAAYSDSYDVQAGGK
                     WAFEGKLEDLSDILTPMKSAFAPNTLETALLYNDVTKKKGYYGFPLKQQSMHVQIWSD
                     MLQQAGFKASDIPTKWEDYWSFWCDKVQPAIRKATGQRIYAVGQPMGVESTDSFQSFY
                     TFMDAYNVKLVDDDGKLLVDDPKVRDGLIHALKDYTDTYIKGCTPPSSTTWKDPDNNV
                     AFHNKTIVMTHNFTISIAAKWLDDANNPALTEEQRAAGKKAYEETIITASFPNKPDGT
                     PIKYRSDVKTGVIFTTAKNKTEGKEFVKFLLQEENLQPYVEGALGRWFPVTTAGQQSP
                     FWQADRHRKAVYTQFTGGTSPFDFTKNYKFTILNNENVWAKAMNRVVSEKVPVEKAVD
                     ELIARIKQVAG"
     gene            2215..3090
                     /locus_tag="VN968_18730"
     CDS             2215..3090
                     /locus_tag="VN968_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL31306.1"
                     /translation="MAITLSTSDHAIPGAPLSERLTMPQVWGIVLLAPYLLVFLAFVV
                     YPVCYGLWLARHPSSYVALYHDPIFARAAVNTLVFLVIGINFKMVVALFLSGFFVQSR
                     SWIKWLSVLFILPWAVPSIPTILSVRFMFNPEWGMINQLIFKFTGEDGPNWLNDPNVA
                     LTMAIMVHIWKSLPFWTLILMTGRLAISADLFEAADVDGATWWQKFRFITWPSMRTLY
                     LTCTLLSMIWTLGDFNSVYLLTGGGPADLTHVLSTLGIRYLRLDQLDLSMAAIVCALP
                     LVLPLVYFMMKRLSR"
     gene            3087..3938
                     /locus_tag="VN968_18735"
     CDS             3087..3938
                     /locus_tag="VN968_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL31307.1"
                     /translation="MKLPSLRDVGTEARLLLIGIPVFIWTMLPIYHMFLFAISPKEDA
                     FAGKFWPDHPTLHNFEIVFRQQHYFLRDFWIQFWNSTVIAVAVGALTLFIATAAAFSI
                     SRLRVPGGRWVMNLALFTYFIPAAFLAVPMYRTMGTYGLLNNHWSLILAMVTIASPYA
                     IWVLKQASDKLPVELDEAAIMDGATTLQLFRLVYVPLMMPSLVAIGTYALLLAWNEYL
                     YAFLLLSRDTDITLPVALGNFLAADDSPWELLMTTGFIYALPPAAIYYAFKRYMVGGL
                     TAGAVKS"
     gene            complement(3940..4755)
                     /locus_tag="VN968_18740"
     CDS             complement(3940..4755)
                     /locus_tag="VN968_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL31308.1"
                     /translation="MARAPNKISPFNLVSLALGLAFLYLPIVILVIYSFNASRLVTVW
                     GGWSVRWYAELFDDRAMLDAASMSFRVATVSATSATLLGTLAAVALSRGERFRGRTLF
                     SGMLYAPLVMPEVITGLSLLLLFVALNAERGFWTVTIAHTTLTMCFVAVVVQSRLGPL
                     DRSLEEAAMDLGCGPARAFLRVTLPLILPAIAAGWMLAFTLSLDDLVIASFTTGPGSA
                     TLPIRIYSEVRLGVKPEINAICTLVIGLIAMVIVVASFASKLSSVRGDSAAPL"
     gene            complement(4755..5669)
                     /locus_tag="VN968_18745"
     CDS             complement(4755..5669)
                     /locus_tag="VN968_18745"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31309.1"
                     /translation="MSVRRTFTRPARISAIAPYLWMALFFLVPFGFVLKISLSQTVIA
                     QPPYAPVFDLAQGWAAIKTALAALSLDNFRLLVTDNLYVLSYLRSLFVAAVSTSILLV
                     IGYPIAYGMARLPQRWQGVAMMLVIVPFWTSFLIRIYAWINILQHDGLLNKILIALHL
                     VSAPVVWLSTDSAMYLGIVYSYLPFMILPLYATLAKMEPAWLEAAGDLGASPWRTFWL
                     VTFPLSLPGVGAGVLLCFIPIVGEFVIPDLLAGSNSMMIGQTLWLEFFTNKDWPVASA
                     AAVVLLLLLLAPLWFYDRLQRRQFEDTR"
     gene            complement(5666..6814)
                     /locus_tag="VN968_18750"
     CDS             complement(5666..6814)
                     /locus_tag="VN968_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31310.1"
                     /translation="MADELPKPELPATATDVPLLRIDAVVKKFGRFKAVDRLSLDIRA
                     GEFFALLGPSGCGKTTLLRMIAGFEMPDEGRILLDGRDIAQVLPHRRPVNMMFQNYAL
                     FPHLTVRDNIAFGLKRAGMARAEMDKRVAGLAALLKLQGLEKRRPDQLSGGQKQRVAL
                     ARALARRPQVLLLDEPLAALDKKLRESTQLELMELQRRLGMTFIIVTHDQEEAMTVAH
                     RIGVMDGGRIEQVATPRELYEAPNSRWIAEFVGDVNVFEGEVDKREAGRLTISTKDAG
                     AIVAAEPRQPITNTAICVAIRPEKVKLSRRGPVSDAGNAHAINRLEGIITEVNYLGGL
                     TTYKVKLDSGAVLRSSMANTARLEFDAYMASQRVVAWFTPDDCVVLER"
     gene            7069..8037
                     /locus_tag="VN968_18755"
     CDS             7069..8037
                     /locus_tag="VN968_18755"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL31311.1"
                     /translation="MKITIVAFEGCMTSAVFGQADAFAVAAYISERRADASWSGHDVR
                     LATPGGKPVRGYGGHLIEPDCSLADADDSHVVLIPPIFNDIEQTLAQETGLVSWLASF
                     PANSKLLASTCTGAFLLAEAGILDGRRVTTNPAFSVLFKQRYPGVRLALDERIVDDNM
                     VICAGATTAYLNLAIHVIDRLAGHDLAVSTAKALSIDRNPESQRPYFLFIAPKDHGDD
                     KVLRLQTWIEVHHRDPISIDDMVGAAGMSVRNLNRRFLSATGISPRQYLRRVRIETAK
                     RLLEEQNVPVDRVAEQVGYGDTRAFIRAFGVLAGFRPASIAKNSGR"
     gene            8162..8938
                     /locus_tag="VN968_18760"
     CDS             8162..8938
                     /locus_tag="VN968_18760"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXL31312.1"
                     /translation="MPAIIHERSGHLAHIRLNRPDNGNRFTTEMFRTLAQLFTALDAD
                     PEIRCILLTARGEDFSLGVDVPDVLPAWAAGKSPFEDSQVNPFGTTGPKRRKPLVTVV
                     HGQCFNAGLELVFASDICIAADNCRFAFHEMRFGVYPFGGGLFRFIRAAGWHAAMRYC
                     LTSEEFGADEARRMNVVALVRPSAEAEAAGLALAREICNAAPLAVQAAIAQAQTWADR
                     GDAAAFAHSVPDIIRLLNSRDAAEAMRAMAEGRAPAFSGQ"
     gene            complement(8927..10429)
                     /locus_tag="VN968_18765"
     CDS             complement(8927..10429)
                     /locus_tag="VN968_18765"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HXL31313.1"
                     /translation="MADYDLAVIGGGLNGVSVARDAAGRGLRVILLEQGDLGAAASSA
                     SPRLVHGGLAMLERRAFFRVRAALAERDIWLRIAPHLVHPMRFAIPTHSDERPSWLLR
                     SGLLLYDRLASRSELPPSATVDVTHHPVGIALKRPFGTAFEYSDCVVDDSRLVIATAM
                     DAAERGAVIRTGARCVRADRLDIWRLVTKDRGHRQIITARALANATGAWTASVAETVL
                     RMPPPNLGTIQIGQIIVRRLFDSDNVYVFQNTDRRLIFASPYQRDFTLIGTVGHAFKG
                     DPAAVSVAASDVVYLCDAANRYFRERVEPSDVIRTVSGANVVDPAGRHAARDGAMAFE
                     ARRGKAPLLTIFGGDVTTSRLRAERAVSRLTPFYPMSPRWTAKAPLPGGDFAWNGFDD
                     EVDGARERWRFLTEAQAQRLVAAYGSRLAAILGDAKTRADLGPAFGPELTGAEVRYLM
                     ACEWARFPDDILWRRSKLGLTMPPGDREALAAFMATAPRTSDARASLLLS"
     gene            complement(10507..12606)
                     /locus_tag="VN968_18770"
     CDS             complement(10507..12606)
                     /locus_tag="VN968_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31314.1"
                     /translation="MVLAAFDLVFLIGVRWMGRILKGLVLVVFAAFVLVFLTNLLFFP
                     SYRHIPSRALAADSASCQDALSPGWDALAAANNNEWTAIDQNSISARKLACSIQHHII
                     PARNLAPGQGDEAPKSLSYDLGFIEFQEDGKPYALRKECDPGEECIRERSIPVRRQAE
                     GQLEALVKQLRGNKSNYVVVFIHGWRHDASIGDGDVANFRIYAAHAARFVADRAALDD
                     AYKDTKVTAIYIGWRGARTDETWLSRKFPWIGDAVGTASAVLTLFDRKPVSEAIAPSA
                     LSALRTIENTLGIDAYSELPRKIPNRMIVFGHSLGGNMLMTALQDDLVKKVALHQPGS
                     YMQPALGDLVVLINPASEASKWTSVQRALWRRLALVNGERRPGKDYEASQVFFRDDQR
                     PFFISATSARNWPPGGRQQLDCSFGDKVQNAENILVHQIADYDWATHDMFPAFKGDLR
                     PLADRLELWGTQRDPNDECAQVRRSFGDRIRHPLRTAAVFISEFIRVLPFMQTDPEQT
                     QTIGNLDPPRSPRGNLRTYGATMKPFGTTHELRTSDRIWQHVARKTSAQDTNREIPLG
                     YGEIMMPEAACPVAAGWLSRAKNEKIQEQAKADPGPRRHYATNWDSYDLKMSTVANEG
                     FPALRFVHGFADSGISPPTRPNDPFWNLRAFDTALAEHDGYMLSSFICAMNQLALDDI
                     TTILPLAPVPAPTPARP"
     gene            complement(12925..15618)
                     /locus_tag="VN968_18775"
     CDS             complement(12925..15618)
                     /locus_tag="VN968_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXL31315.1"
                     /translation="MVDKDWLAGGKNLIPAQAVVCLIAAVAPFGSAHGASGDFSPSGF
                     VGSADPNAIWEMLIAGIAVSSFLAAIALWVLSALRKVKRSELRRNAFVRSALNNLSHG
                     VVMTDPRRRIVFCNDRYLEIYGLARSDLAKDMTGPELLALQLKRGVLDVSVDDFYVHA
                     SAPEGLITELPGGRSVLVKYFALPNGGSVATHEDCSEQRKLSRQLASTKQFLESMLDN
                     VPVCVAAKSIEDGRYIFANRAFERFSRFSRDHILGKRADEIFQAETAAGIAAADRSAL
                     NSSDGQFRNEIVVQRGSKKRVLASTRVIVRDDNKQPEFLIALFDDVTDRQSLSKELED
                     TKKFLELVVDNIPVSLIVERVSDGRYLLANRSAEAILNRRREDATGLTAADIFNPREA
                     KLIIARDEAAIKKRGLLTEEHPISTKDGLRLFLTRRMTVLDDTGKPQYLIKTHEDVTD
                     RRQTESRMAHMAYHDGLTDLPNRAAFLQALAQMIEACEGTDEEFAVLSVDLDGLKEIN
                     DVFGHAVGDKLLIEVSRRIQTSARGGVVARLSGDEFGLIIDGKQPAAGKALAEQLAEV
                     LAQEFPIDGKSVRIGVTTGISVFPHNGADAASLLANAGAALFRAKAKSRGSISVYEPE
                     MDQQIRDRRVLHQDLSLAIKNGELSLHYQPQAASGQTVASSEVIGFEALARWLHPVRG
                     FVPPGDFIPLAEESSLIVEMGEWILREACREAASWSKPLQIAVNLSPAQFMHGDLVSL
                     VHSILLETGLSPGRLELEITEGVLIEDFDRGLALLRRLKALGVRISMDDFGSGYSSLS
                     YLQAFPFDKIKIDRAFVMNLERNPQSAAIVRAVIGLGHGLEMSIVAEGVETQEQLGFL
                     ADEGCDAVQGYFLGKPAPIGQYAALVGRSGAAAMELARKTG"
     gene            15919..17022
                     /locus_tag="VN968_18780"
     CDS             15919..17022
                     /locus_tag="VN968_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL31316.1"
                     /translation="MDKVECVVIGAGVIGLAVARRLAQAGREVIILEAAEGIGTVTSS
                     RNSEVIHAGIYYPAGSLMARMCVSGKRALYRYCRDHGIPHRNCGKLIVATTPKEATKL
                     QSIRAHAEANGVLDLQTLSGDAARTLEPALNCDAALLSPSTGIIDSHAFMLALQGDAE
                     AAGAACAFHTPLLHAKALADRIELDAGGNAPISLECKLLVNAAGLSASAVARSINGMP
                     IDLIPAAYLAKGNYFSCSARSPFSRLIYPVPEPGGLGVHLTLDMAGQARFGPDVEWVD
                     SIDYAVDPARAEKFYPAIRRYWPTLPDGALMPSYSGIRPKIVPPAVATQDFLIQGPRD
                     HGVPGLINLFGIESPGLTSSLAIADHVGELAAM"
     gene            complement(17179..17364)
                     /locus_tag="VN968_18785"
     CDS             complement(17179..17364)
                     /locus_tag="VN968_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HXL31317.1"
                     /translation="MAIEAHLVELERKHKVLENELHEALVHLSTDDLEIVELKRRKLM
                     VKDEIERLRHTASETLH"
     gene            17743..17949
                     /locus_tag="VN968_18790"
     CDS             17743..17949
                     /locus_tag="VN968_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HXL31318.1"
                     /translation="MTNEDERELESELARLQQEHRDLDAAIDALHQSPAPDLLRLQRL
                     KKRKLQLRDRIAFIEDQITPDIIA"
     gene            complement(17984..18364)
                     /locus_tag="VN968_18795"
     CDS             complement(17984..18364)
                     /locus_tag="VN968_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL31319.1"
                     /translation="MGLVETKRPVVLIVEDEFLLRMDAVDMIEAAGFEVVEAANADEA
                     IEILEARRDITVVFTDIQMPGSMDGLKLARAVRGRWPPIRIIATSGHVHVSETELPEG
                     GRFLPKPYNPMQVTGVLRELISGA"
     gene            18690..19103
                     /locus_tag="VN968_18800"
     CDS             18690..19103
                     /locus_tag="VN968_18800"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXL31320.1"
                     /translation="MRSRPPCATRSGRGYEAVVIDNVAEDAAYCGHHTPAKYGFQSYI
                     SMPIILADGTFFGTLCALDPRPAQLDTPETIGMFKLFPELIAIHLEAVDRLAASEARL
                     LSERETSELARAIHRRARPRSAQSARLDRRGGRKC"
     gene            19097..19366
                     /locus_tag="VN968_18805"
     CDS             19097..19366
                     /locus_tag="VN968_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31321.1"
                     /translation="MLTKEASREAAGEILGLIQKSVARMSALIDNVLDFACGRLGSGI
                     ALKRSPQSLQPVLDHVIAELRASLPERNIEKMFEKIPAGELGRGP"
     gene            19424..19636
                     /locus_tag="VN968_18810"
     CDS             19424..19636
                     /locus_tag="VN968_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31322.1"
                     /translation="MSESSSQSDVSHYEQACDQAIAMCDGNMRSTIKALIMANEYLES
                     ELRELQAAVESGCAAAGLPHASYDAA"
     gene            19664..19915
                     /locus_tag="VN968_18815"
     CDS             19664..19915
                     /locus_tag="VN968_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31323.1"
                     /translation="MVDVIYYVALPFLQDDTGAPVAGAAEECQSSSGALRRAEVLSKA
                     AGSVGAVAFSRTGDPMIGEFSDAKLLRKFGDVPDDLSGL"
     gene            complement(19951..21108)
                     /locus_tag="VN968_18820"
     CDS             complement(19951..21108)
                     /locus_tag="VN968_18820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HXL31324.1"
                     /translation="MTVKPTPFTLRIADADIADLRDRLARARFPDQAPGDAWAYGTDV
                     AYLRGLAEYWRSDFDWRAEEAALNAFPQFRVPLDGVDLHYLHVPGVGPDPMPLLLLHG
                     WPGSVFEFLEFIPRLTDPARFGGDARDAFTVVAPSLPGYGLSFQPGQRRFGVPEIADC
                     VAALMHDVLGYHSFGAQGGDWGAAVSSRLGYAHANRMIGIHINLMMAAGRDPSAFPDP
                     TEEERRYLGELAHWTREESGYQWIQGTRPQTLAFGLTDSPTGLAAWIVEKFRAWSDCD
                     GDVELAIGRDRMLADISLYWFTRAIGSSFWPYYARLHGSPILPAGDTIVVPTGYAQFP
                     REILKPPRSAAARVFTDIRRWSIMPKGGHFAALEQPELLAGEVRAFFRDLR"
     gene            complement(21206..21910)
                     /locus_tag="VN968_18825"
     CDS             complement(21206..21910)
                     /locus_tag="VN968_18825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXL31325.1"
                     /translation="MFMKKKTKATAPGTAKRRKSGVAERKSAPQRARRVKPASASAPR
                     LAVAPNEAKLTIRRLKVQLAQALARIDELEASADTDFLLDIPNRRGFERELNRAVAYI
                     KRYHASAALIVLDVDRLKPINDAFGHAAGDQVLKAIVAALLRHVRASDVIGRLGGDEF
                     ALLLWNLSETDAHAKAAALEQAIDRLSFVFRGRTVAAGASAGVAVLGTHAEAARALEE
                     ADSAMYVRKAQRRHEA"
     gene            22042..22530
                     /gene="purE"
                     /locus_tag="VN968_18830"
     CDS             22042..22530
                     /gene="purE"
                     /locus_tag="VN968_18830"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HXL31326.1"
                     /translation="MTAPIAIIMGSQSDWETMRHAADTLTALGVACEKHIISAHRTPD
                     RLFAFAKGAKATGFKIIIAGAGGAAHLPGMTASLTELPVFGVPIESRALSGLDSLYSM
                     VQMPAGVPVGTLAIGKPGAINAALLAASVLALGDPALAGRLVAWRKAQTEAVAERPEG
                     SA"
     gene            22527..23636
                     /locus_tag="VN968_18835"
     CDS             22527..23636
                     /locus_tag="VN968_18835"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HXL31327.1"
                     /translation="MTSTNQVKLKPGDTIGILGGGQLGRMLAMAAARLGLRCQVFSPD
                     PDSPAFDVVLNATCAEYADVEALELFANDVDVITYEFENVPAASAMILAARRPVLPDR
                     DVLETTQDRLVEKDFVKRLGIGTADYADVSSAAELHAAIGTIGLPAVLKTRRFGYDGK
                     GQAIIRDGDDPGQVWEDLGTKSAILEAFIPFEREISVIAARSASGDVECFDVTENEHS
                     DHILKISRAPAAISEALAAEARSVAERIANALDYVGVLAVEMFVVQADDGPTVLVNEI
                     APRVHNSGHWTLDGASISQFEQHIRAIAGWPLGKPVRHGPVVMTNLIGDEINGYEQWL
                     TVPGATVHLYGKGSPRPGRKMGHVTQVGAAPSKTA"
     gene            23789..24094
                     /gene="rpsU"
                     /locus_tag="VN968_18840"
     CDS             23789..24094
                     /gene="rpsU"
                     /locus_tag="VN968_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HXL31328.1"
                     /translation="MQVLVRDNNVDQALKALKKKMQREGIFREMKLRGHYEKPSEKKA
                     REKAEAVRRARKLARKKLQREGLLPMKPKPVFGAGPGADRGGAGGRGGPGGGPRGPR"
     gene            24278..24871
                     /locus_tag="VN968_18845"
     CDS             24278..24871
                     /locus_tag="VN968_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL31329.1"
                     /translation="MTVVALLALCGCSVNLGSLSPELDKDPAPKPAPTETSTGDAQAD
                     TARGQALARSGKTEEALAEFDKAIALDPHCAEALYDRGLLYQAEKQHQLAIDDFTSAN
                     GLTPQRAEPLLGRAISYLALDRIREAAADLDEAVQADPTNAQIWTTRGLAYERLGDKT
                     RAAGSYGRAVGIRPKDEAARNGFARVGGKAGQTYEPF"
     gene            complement(24873..25832)
                     /locus_tag="VN968_18850"
     CDS             complement(24873..25832)
                     /locus_tag="VN968_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31330.1"
                     /translation="MLKYLSKFTLDVLPSLLATVIGAYIVNHYINKPDAPVAAVVSTA
                     EPKADPKTPEAVKAEAKTDANKTDAKTPETSTDVANIPEPGVRAKGISEKAVLDRAGA
                     EKSADKPAETASIPPDTRRHQAAPREKTVAKTVPAPQPSAPAVVPVVAAAPNPVPPAE
                     AAAPATPVDEHRDANELARAAIERLRGLTADTSPRAPEVARAPDAARIPDAPHVVYAA
                     PIESPRAVPAPAVRPLPPPIMVSTPPREPFDPAAGSPQGRPPYDAARNDDPHRPTPPA
                     DIPVSQPLDLRAEAIEPLPKERRNVAEDVLSAAKSVFHAVLPK"
     gene            complement(25942..26226)
                     /locus_tag="VN968_18855"
     CDS             complement(25942..26226)
                     /locus_tag="VN968_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL31331.1"
                     /translation="MLSAKSDIQVDTPEVRVTEWRLAPGSATGHHIHEMDYVIVPVTA
                     GEMTIVAPGGERSKAQLGLGKSYFRKAGVEHDVLNETATEIVFLEVELKP"
     gene            complement(26245..27342)
                     /locus_tag="VN968_18860"
     CDS             complement(26245..27342)
                     /locus_tag="VN968_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HXL31332.1"
                     /translation="MSAYGPMPRSAWIFPVLAVLFFAGATALGLTFTPSAAGLGFAVV
                     LLIILFGTVFAAVHHAELIAERIGEPYGTLLLTLAVTIIEVALIATIMLGEKPVPTLA
                     RDTVFAVVMIVCNGLVGICILAGGLRYREQDVQVTGSSLYLSVLIVMATITLIMPNYT
                     LATPGPIYSAGQLGFVSVVTIILYGVFLYTQTIRHRSYFVSEATGAADGGTHVSNRLL
                     GLSMALLLISLLAVVLLAKKFSLVVDFATARIGAPPAFAGVLVALLILLPESVAAVAA
                     ARKNDLQKSINLALGSSLATIGLTIPAVAVAAYALDKELVLGLDAQAMVLMAMTFILS
                     MLTFGTGRTNILFGLVHMVVFAVFVFLVFAP"
     gene            complement(27397..28203)
                     /locus_tag="VN968_18865"
     CDS             complement(27397..28203)
                     /locus_tag="VN968_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL31333.1"
                     /translation="MIVLKWLVILAIVGYLGGLAVVFFKQREFVFPIPQTLRTAPEAA
                     GFPEAEEHVLNTADGEKVIIWHVPAQPGHAVVIYFPGNGDFLAGNVGRFRAIAADGTG
                     LVALSYRGYAGSSGQPSEQGLLQDAAAAYAFTAARYPPDRIVLWGFSLGTGAAVAVAA
                     DQPVGKLILEAPYTSMVDVAARHFPVLPVRWLLRDRFHSDQRMARVTAPLLVMHGERD
                     LTIPIEFGERLFGLAPGPKQFVRFPLGGHDDLGNHGAISTARQFIGGPNG"
     gene            complement(28222..>29343)
                     /locus_tag="VN968_18870"
     CDS             complement(28222..>29343)
                     /locus_tag="VN968_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HXL31334.1"
                     /translation="HSLVGMAAVLVAAGAFYAPEAFDIGTPGNIHGASLVEMSLGVAI
                     GALTFTGSVIAFAKLSGRMSGAPIILPARHVINVALAIALVVFIVGLVLTGSALDFWL
                     ITIIALALGALLIIPIGGADMPVVISMLNSYSGWAAAGIGFTLGNSALIITGALVGSS
                     GAILSYIMCHAMNRSFISVILGGFGGETAAAGAGAGGEVRPVKLGSADDSAFIMKNAQ
                     KVIIVPGYGMAVAQAQHALREMADQLKKDGVEVKYAIHPVAGRMPGHMNVLLAEANVP
                     YDEVFELEDINSEFAQADVAFVIGANDVTNPAAEDDSKSPIYGMPVLQVWKAGTVMFI
                     KRSLASGYAGIDNPLFYRDNTMMLLGDAKKMTENIVKAL"
BASE COUNT         5655 a         9251 c         9162 g         5275 t
ORIGIN      
        1 ccggggagag cacgacataa gctgttaaaa ccattgcgtg cgggaatgcc gggcgttccg
       61 gtgtactcgt tgttactcgt gtgcgttcta ccaatacaaa ctgcacacga ggccgcgggt
      121 gcaacgggca cccggcattc cgcacgccct ccagggggcg aaagattcat caacgactcg
      181 ggcgctttgc gttgcgaggg cgtgaaactg cgtctgaaat cgttttggct gtttgaaaat
      241 tgaagttggt gcgtgtgaag cgatcctcaa cctcagccct cgaaacgaac tcgtcatgcc
      301 cggacttgat ccgggcatcc atcaatcttc gcaagaaagc cctttcgaag aagatggatc
      361 accgggtcaa gcccggtgat gacgatcttg ttcaagcgac ccgttggctc gcaatgacgg
      421 aaagcacgta cggccgatcg agcagcttaa tcctccgttc tccgcagaca gacaggcggg
      481 tcacactgcg gtaccccctg ggccggcgtc tcatacgcaa gacgtggacg tgccatctag
      541 atcgcgccgc ggcaatgggc taggatttta cccaaccggt taataaaact cggcggatca
      601 gggagaataa cgtgacaccc aaggcaatac gcgcgctttc attgacggtc gccgccatcg
      661 gacttctcta tgccgcggcg ccggcgatcg cgcaggaaaa gaaaatcacg gtctggtggg
      721 gaaagggttt ttacaagtcc gaggacgacg cgctgcttga agcgatcaag aagttcgagg
      781 ccaagaccgg catcaaggtc gagctgtcgc aatacgccat tcaggacatg attccgaaga
      841 cggtggcggc attggactcc ggcacggtcc ccgacgccgc ctattccgac agctacgacg
      901 tgcaggcggg cggcaaatgg gcgttcgagg gcaagctcga ggatctctcc gacatcctga
      961 cgccgatgaa atcggcgttc gcgccgaaca cgctcgagac cgcgctgctc tataacgacg
     1021 tcaccaagaa gaaaggctat tacggcttcc cgctgaagca gcagagcatg cacgtccaga
     1081 tctggagcga catgctgcaa caggccggct tcaaggccag cgacatcccg acgaaatggg
     1141 aagattactg gtcgttctgg tgcgacaagg tgcagccggc catccgcaaa gccaccggcc
     1201 agcgcatcta cgccgtcggc cagccgatgg gcgtggaatc caccgactcg ttccagtcgt
     1261 tctatacctt catggacgcc tataacgtca agctcgtcga tgacgacggc aagctgctgg
     1321 tcgacgatcc caaggtcagg gacgggctta tccacgcgct gaaggattac accgacacct
     1381 acatcaaggg ctgcacaccg ccctcctcca ccacctggaa ggaccccgac aacaacgtcg
     1441 cgttccataa caagaccatc gtgatgaccc acaacttcac gatctcgatc gcggcgaagt
     1501 ggctggatga tgccaacaac ccggcactga ccgaagagca gcgcgccgcc ggcaagaagg
     1561 cctacgagga aaccatcatc accgcctcgt tcccgaacaa gccggatgga acgcccataa
     1621 aatatcgttc cgacgtcaag accggcgtga tcttcacgac cgcaaagaac aagacggaag
     1681 gcaaggaatt cgtcaagttc ctgctgcagg aggagaacct gcagccttat gtcgaaggcg
     1741 cgctcgggcg ctggttcccg gtcaccacgg ccggacagca gagcccgttc tggcaggctg
     1801 accgtcatcg caaagcggtc tatacccagt tcacgggcgg cacgtcgccg ttcgacttca
     1861 ccaagaacta caagttcact atcctgaata acgagaacgt ctgggccaag gcgatgaacc
     1921 gcgtggtgag cgaaaaggtg ccggtggaaa aggcggtcga cgaattgatc gcccgcatca
     1981 agcaggtcgc ggggtagagc ttgacaatgc tggccgccac aaactccagt gcatcgtcgt
     2041 cccggcgaac gccgggaccc ataaccacgg gccgtgccgg ttgcgcgaaa gccgtcgaac
     2101 tgcatcggcc aacacaagcg acatggcgta tgggtcccgg cgttcgccgg gacgatggcg
     2161 tgctggaaaa ttctctcgct gcggccggca tctcctggga gtagagtacg gcatatggcg
     2221 atcacgctct cgacctccga tcacgcgata cccggcgcgc cgctgtcgga acgcctgacg
     2281 atgccgcaag tctggggcat cgtgctgctg gcgccttacc tgctcgtatt cctggctttc
     2341 gtggtctatc ccgtctgcta cgggttatgg ctcgcgcgcc atccatcgag ctatgtcgcg
     2401 ctctatcacg atccgatttt tgcccgcgcc gcggtgaata cgctggtctt tctcgtgatc
     2461 ggcatcaatt tcaagatggt ggtggcgctg tttctgtcgg gcttcttcgt gcagtcgcgc
     2521 tcgtggatca aatggctgtc ggtgctgttc atcctgccat gggcggtgcc ttcgatcccg
     2581 accatcctgt cggtgcgctt catgttcaat cccgaatggg gcatgatcaa tcagctgatc
     2641 ttcaagttca ccggcgaaga cggtccgaac tggctcaacg atcccaatgt cgcgctgacg
     2701 atggcgatca tggtgcacat ctggaaatcg ctgccgttct ggaccctgat cctgatgacc
     2761 gggcgtctgg ccatatcggc tgatctgttc gaggcggccg acgtcgacgg cgcgacctgg
     2821 tggcagaaat tccgcttcat cacctggcca tcgatgcgga cgctgtacct cacctgcacg
     2881 ctgctctcga tgatctggac gctcggcgac ttcaacagcg tctatctcct caccggcggc
     2941 ggcccggccg atctcaccca cgtgctgtcc accctcggca tccgttacct gcggctcgac
     3001 cagctcgatc tgtcgatggc cgcgatcgtg tgcgcgctgc cgctggtgct gccgctggtc
     3061 tatttcatga tgaaacggtt gtcgcgatga agctgccttc cttacgcgat gtcggcaccg
     3121 aggcgcggct gctgctgatc ggcattcccg tgttcatctg gaccatgctg ccgatctatc
     3181 acatgttcct gttcgcgatc tcgccaaagg aagacgcgtt cgcgggcaaa ttctggcccg
     3241 atcatccgac gctgcacaat ttcgaaatcg tgtttcgcca gcagcactat tttctcaggg
     3301 atttctggat tcagttctgg aattcgacgg tgatcgcggt cgcggtcggc gcgctgacgc
     3361 tgttcatcgc gaccgcggcg gcgttctcga tctcgcggct gcgcgtcccc ggcggccgct
     3421 gggtgatgaa cctggcgctg ttcacctact tcatcccggc ggcgttcctc gccgttccga
     3481 tgtatcgcac catgggcacc tatgggctgc tcaacaatca ctggtctttg attctggcga
     3541 tggtgacgat cgcgtcgcct tacgcgatct gggtgctcaa gcaggcttcc gacaagctgc
     3601 cggtggaact cgacgaagcc gcgatcatgg acggcgccac gaccttgcaa ctgttccggc
     3661 tggtctatgt gccgctgatg atgccgtcgc tggtggcgat cggcacctat gcgctgctgc
     3721 tcgcctggaa cgaatatctc tatgcgttcc tgctgttgtc aagggacacc gacatcaccc
     3781 tccccgtcgc gctcggcaac ttccttgccg ccgacgattc gccgtgggag ctattgatga
     3841 ccaccggctt catttacgcg ctgccgccgg ccgcgatcta ctacgcgttc aagcgctaca
     3901 tggtgggcgg cctgaccgcc ggcgcggtga agtcgtgacc tatagcggcg cggcgctgtc
     3961 gccgcgcacg ctcgataatt tcgaggcgaa cgacgccacc acgatcacca tcgcgatcaa
     4021 tccgatcacc agcgtgcaga tcgcgttgat ctccggcttc accccgagcc gcacctccga
     4081 atagatccgg atcggcagcg tcgccgaacc gggacccgtg gtgaagctcg cgatcaccag
     4141 atcgtccagc gacaacgtga acgccagcat ccagcccgcg gcgatcgcgg gcaggatcag
     4201 cggcagcgtg acgcggagaa acgcccgcgc cggaccgcaa ccgagatcca tcgccgcctc
     4261 ctccaggctg cgatcgagcg ggccgagccg cgactgcacc accacggcga cgaagcacat
     4321 ggtgagcgtg gtatgggcga tggtcacggt ccagaagccg cgctcggcgt tcagcgccac
     4381 gaacaacaat aatagcgaaa gcccggtgat gacttcgggc attaccagtg gcgcatagag
     4441 catgccggaa aacagcgtgc ggcccctgaa acgctcgccc cgcgacagcg ccaccgcggc
     4501 gagggtgccg agcagcgtcg ctgatgtggc cgataccgtc gccacgcgga aactcatcga
     4561 cgccgcgtcc agcatggcgc ggtcgtcaaa caattcggcg taccagcgca ccgaccagcc
     4621 gccccacacc gtcaccagcc gcgaggcgtt gaacgaatag atcacgagaa tgacgatcgg
     4681 caggtacaga aaagcaaggc ccagcgccag cgagaccaag ttgaacggcg agatcttgtt
     4741 cggcgcgcgg gccattatcg cgtgtcttcg aactggcggc gctgcagccg gtcgtagaac
     4801 cacaatggcg ccagcagcag caggagcagc accaccgcgg ccgccgaggc caccggccag
     4861 tccttgttgg tgaaaaattc cagccacagc gtctggccga tcatcatcga attggaaccg
     4921 gccagcaggt ccggtatcac aaactcgccg acgatcggaa tgaagcacag caatacgccg
     4981 gcgccgacgc ccggcaacga cagcggaaac gtcaccagcc agaacgttcg ccacggcgag
     5041 gcgccgagat cgcccgccgc ctccagccac gccggctcca tcttggcgag cgtcgcatag
     5101 agcggcagga tcatgaacgg cagataggaa tagacgatgc cgagatacat cgcgctgtcg
     5161 gtggacagcc acaccaccgg cgccgagacc agatgcagcg cgatcaggat cttgttgagc
     5221 aggccgtcgt gctgcaggat attgatccag gcatagatcc ggatcagaaa cgaggtccag
     5281 aacggaacga tcaccagcat catcgcgacc ccctgccagc gctgcggcag ccgcgccatg
     5341 ccgtaggcga tggggtagcc gatcaccagc aggatcgagg tcgagaccgc ggcgacaaac
     5401 aggctgcgca gataggacag cacgtaaagg ttgtcggtga ccagcaacct gaaattatcc
     5461 agcgacagtg ccgccaacgc cgtcttgatc gccgcccaac cctgcgccag gtcgaaaacc
     5521 ggcgcatagg gcggctgcgc gatcacggtc tgcgacaggc tgatcttcag cacaaacccg
     5581 aatggcacca ggaagaacag cgccatccac agataggggg cgatcgcgct gatgcgcgcc
     5641 ggacgggtga aggtgcggcg gacgctcatc gctccagcac cacgcaatcg tcgggcgtga
     5701 accacgccac cacacgctgg ctggccatgt aggcgtcgaa ttcgagccgc gccgtgttgg
     5761 ccatcgacga acgcagcacg gcgccgctgt cgagcttcac cttataggtc gtgagaccgc
     5821 cgagatagtt gacctcggtg atgatcccct ccagccggtt gatggcatgc gcattgcccg
     5881 cgtcggatac cgggccgcgg cgcgacagct tgaccttctc ggggcggatg gcgacgcaga
     5941 tcgccgtgtt cgtgatcggc tggcgcggct ccgcagcaac gatcgcgccg gcgtcctttg
     6001 tcgaaatcgt caggcgaccg gcctcgcgct tgtcgacttc tccctcgaac acattgacgt
     6061 cgccgacgaa ctcggcgatc cagcgcgaat tgggggcctc gtagagttcc cgcggcgtcg
     6121 cgacctgctc gatacggccg ccatccatca cgccgatccg gtgagctacc gtcatcgctt
     6181 cttcctggtc gtgggtgacg atgatgaagg tcatgccgag gcgccgctgc aactccatca
     6241 attcaagctg ggtgctttcg cgcagtttct tgtcgagcgc ggcgagcggc tcgtcgagca
     6301 gcagcacctg cggacggcgc gccaatgcgc gcgccagcgc cacgcgctgc ttctggccgc
     6361 cggagagttg atcgggccgc cgcttctcca gaccctggag cttgagcagc gcggccaacc
     6421 cggccacgcg cttgtccatc tcggcacggg ccatcccggc gcgcttcagc ccgaacgcaa
     6481 tgttgtcccg cacggtgagg tgaggaaaca gcgcgtagtt ctggaacatc atgttgaccg
     6541 gacgccggtg cggcagcacc tgggcgatat ccctgccgtc aagcaggatc cggccttcgt
     6601 ccggcatctc gaagccggca atcatccgca acagcgtggt cttgccgcaa ccagagggac
     6661 cgagcagcgc gaaaaactct ccagccctga tgtcgagcga cagccgatcc acagctttga
     6721 agcggccgaa cttcttgacc accgcgtcga tccgcagcag cggaacgtcg gtcgcagttg
     6781 ccggcagttc gggttttggt agttcgtcag ccatgatcct cgccgcccca tcgtagcggc
     6841 acgctagcgg cggatcgggc ccggctcaac cgtatcagcg tcgcaccgca acaatattgt
     6901 cggtggcgtt ggtctcgtct ccggtaaggc tctgcaactg ccgagcgatg tcggccgtcg
     6961 atcgcgagac gggtcagacg ctgacgcaac tcaaagaggc ggaaaatgtt gacgcaatcg
     7021 aatcggaaac gcagtgctgc ggacaaccgg ttcgtgtatt ccacccctat gaaaatcacg
     7081 atcgtcgcct tcgaaggttg catgacctcg gccgtgttcg gtcaggcgga cgcctttgcc
     7141 gttgccgcgt acatctccga gcggcgcgcc gatgcaagct ggtccggcca tgatgtccgc
     7201 ctggccacgc cgggcgggaa acccgtgcgg ggctatggcg gacacctgat cgagcccgat
     7261 tgctcgctcg ccgatgctga cgacagtcat gtcgtgctga ttccgcctat ttttaacgac
     7321 atcgaacaga ccttggcgca ggaaaccggc ctcgtctcct ggttggcgtc atttccggca
     7381 aattcaaagc tgctggcttc aacctgcacc ggcgcgttcc ttctcgccga agccggcatt
     7441 ctcgacggcc ggcgcgttac caccaatccg gcctttagcg tgcttttcaa acagcgctat
     7501 ccgggcgtgc gcctggcatt ggacgaacgt atcgtcgatg acaacatggt catctgcgca
     7561 ggtgcgacca ctgcctatct caacctcgcg atccatgtga tcgatcggct ggccggccat
     7621 gatcttgcgg tatcgacggc aaaggcgctg tcgatcgacc gcaacccgga atcgcagcga
     7681 ccctatttcc tgttcatcgc ccccaaggat cacggcgacg acaaggtgct gcgacttcag
     7741 acatggatcg aggtccatca tcgggacccg atcagcattg acgacatggt gggcgctgcc
     7801 ggcatgagcg tgcgcaattt gaaccgccgc tttctttccg ccaccggtat atcgccgcgg
     7861 cagtacctcc gcagggtccg catcgaaacc gccaagcggt tgcttgagga acagaacgtg
     7921 cccgtcgacc gggtggccga acaggtcggt tacggcgaca cccgcgcctt catccgggca
     7981 ttcggggttc tcgccggctt tcgcccggcc agtatcgcca aaaattccgg gcgctgaggc
     8041 gcgaactgcc gcttgagccc atcaagggct gaaacggcaa cctgaatggc cgcgcctgcc
     8101 atggtcgccc gcagcccgat cggggacatt gccgttacgt ccagtgcacg ggagccccgc
     8161 gatgcccgcc atcatccatg aacgatccgg acacctcgcc cacatcagat tgaaccgtcc
     8221 cgacaacggc aatcggttca cgacggagat gtttcgcacg cttgctcagt tgttcacggc
     8281 gctggacgcc gatcccgaga ttcgctgcat cctgctcacc gcacgcggcg aagatttttc
     8341 gctcggcgtc gatgtgccgg atgtgctgcc ggcctgggcc gcgggcaaaa gccccttcga
     8401 ggacagccag gtcaatccgt tcggcaccac cggtcccaaa cgccgcaagc cactcgtcac
     8461 cgtcgtccac ggccagtgtt tcaatgcagg actcgaactg gtgttcgctt cggatatctg
     8521 tatcgccgcc gacaattgcc gctttgcgtt tcacgaaatg cgcttcggcg tctatccgtt
     8581 cggcggcggg cttttccgct ttattcgggc ggcgggctgg catgcggcga tgcgctattg
     8641 cttgacgtcg gaggaattcg gcgccgacga ggcgcgccgc atgaatgtgg tagcgctggt
     8701 gcggccctca gccgaggccg aagccgcagg tctcgcgctt gcccgggaaa tctgcaacgc
     8761 cgctccgctt gccgtgcagg ccgccatcgc ccaggcccag acatgggcgg atcgcgggga
     8821 cgcggccgcc ttcgcccatt cggtaccgga tatcatccgg ttgctgaact cacgcgatgc
     8881 ggctgaggcg atgcgggcga tggccgaggg gagagctccg gcgttttcag gacagtaaga
     8941 gcgaagcgcg agcatccgag gttcggggcg cggtcgccat gaacgccgcc agcgcttcgc
     9001 gatccccggg cggcatggtc agaccgagtt ttgagcgccg ccacagaata tcgtctggga
     9061 aacgcgccca ttcgcatgcc atgagatagc gcacctccgc gcccgtcagt tccgggccga
     9121 aggcgggacc gaggtcggcg cgggttttgg cgtcgccgag gatggccgca agacgcgagc
     9181 cataggcggc gaccaggcgc tgcgcctgag cctcggtcag aaaccgccac cgctcgcgcg
     9241 cgccatcaac ctcgtcgtcg aacccattcc acgcaaaatc gcctcccggc agcggcgcct
     9301 tcgcggtcca gcgcggcgac atcggataga acggcgtgag cctggagacg gcccgctccg
     9361 cgcgcagccg cgaggtcgtg acatcgccgc caaagatcgt cagcagcggc gccttgccgc
     9421 gccttgcctc gaacgccatc gcgccatccc gcgcggcgtg ccggcctgcg gggtccacca
     9481 cgttggcgcc ggaaacggtc ctgatcacgt cggatggttc gacccgctcg cgaaaatagc
     9541 ggttggccgc gtcgcaaaga tacacgacat cgcttgcggc cacggatacg gcggccgggt
     9601 cgcccttgaa cgcgtgcccc acagtgccga tcagcgtgaa atcgcgctgg taggggctgg
     9661 cgaaaatcag ccgccggtcc gtgttctgga aaacatagac gttgtcggaa tcaaacagcc
     9721 gccggacgat aatctggccg atctggatcg tgccgaggtt cggcggcggc atccgcaata
     9781 ccgtctccgc aaccgacgcg gtccaggcgc cggtagcgtt ggccagcgcc cttgccgtga
     9841 tgatttgacg atggccgcga tctttcgtca ccagccgcca gatgtcgagc cggtcggcgc
     9901 gaacgcaacg cgcgccggtg cggatcacgg cgccccgctc ggcggcatcc atggcggtgg
     9961 cgatcaccag ccgcgaatcg tcgactacgc agtcggaata ttcaaaggcg gtgccgaatg
    10021 gccgcttcag cgcgatgccg accggatggt gcgtgacatc gacggtggct gatggcggca
    10081 gctcgctgcg cgaggccagc cggtcgtaga gcaataaccc cgaccgcagc agccacgatg
    10141 ggcgctcgtc ggaatgcgtg gggatcgcaa agcgcatcgg gtggaccaga tgcggcgcga
    10201 tccggagcca gatgtcgcgc tcggccagcg cggcacggac gcggaaaaac gcccggcgct
    10261 ccagcatcgc cagaccgccg tggaccagcc gtggcgaggc cgaggacgcg gcagcgccga
    10321 ggtcgccctg ttcgagcagg atcactcgca ggccgcgtcc cgcggcatcg cgtgcaacgc
    10381 tgacgccgtt caagccaccg ccaatgaccg caagatcgta gtccgccacg tcaggaaagc
    10441 tccaactccg cccaccatga caagcggtgc gggcaaaact aagccgttgc gcgcaggaag
    10501 caaattttat ggacgcgcag gagtcggagc gggaactgga gctaacggaa gaatggttgt
    10561 gatgtcgtca agggcgagtt gattcatggc gcagataaat gacgaaagca tgtatccatc
    10621 gtgctctgcg agcgcggtgt cgaatgcccg caaattccag aacggatcgt tgggacgagt
    10681 cggcggggaa atcccgctat cggcaaagcc gtggacgaaa cgcagggccg ggaagccttc
    10741 gttggcgacg gtgctcatct tcaggtcata actgtcccaa ttggtcgcat aatgccggcg
    10801 cgggcccggg tctgctttcg cctgctcctg aatcttttcg ttcttggcgc gcgacagcca
    10861 gcctgccgcg accgggcatg ctgcctcggg catcatgatc tccccgtagc caagcggtat
    10921 ttcccggttt gtgtcttgcg ccgacgtttt gcgggcgacg tgctgccaga ttctgtccga
    10981 tgtgcgcaat tcatgggtgg tgccgaacgg cttcatggtc gcaccatagg tccgcagatt
    11041 gcctcgcggc gaacgtggcg ggtcgaggtt gccgatggtc tgggtctgct ccgggtcggt
    11101 ctgcatgaac ggcaatacgc ggatgaactc gctgatgaag acagccgcgg ttcgcaacgg
    11161 atgacgtatc ctgtcaccga acgaccttct gacctgagcg cattcgtcgt tgggatcgcg
    11221 ttgcgtgccc cataattcga gccggtcggc caagggacgg agatcgccct tgaacgccgg
    11281 aaacatgtcg tgcgtcgccc aatcgtaatc agcgatctgg tgaaccagaa tattttcggc
    11341 gttttgcact ttgtcgccga agctgcagtc cagctgctgc ctgcccccgg gcggccagtt
    11401 gcgcgccgag gtcgccgaaa tgaagaaggg acgctgatcg tcacggaaaa acacctgcga
    11461 cgcttcataa tccttgccgg gacgccgctc gccgttgacc aaggcaagcc tgcgccacag
    11521 cgcccgctga acggaagtcc atttggaagc ctccgaagcc ggattgatca gcacgaccag
    11581 atcaccgagc gcgggctgca tgtaggaacc gggttggtgc agggcgactt tcttgacgag
    11641 gtcatcctgc agggcagtca tcagcatgtt gccacccaga gaatggccga aaacgatcat
    11701 tcggttcggg attttccgcg ggagctcgct atacgcatcg atccccaggg tgttttcaat
    11761 cgtccgcagc gccgacaacg ccgagggggc aatcgcctcg ctgaccggct tgcgatcgaa
    11821 caacgtcaga accgcggaag ccgtaccgac ggcatccccg atccagggaa actttcggga
    11881 tagccaggtc tcgtcggtcc gcgcacccct ccacccgatg tagatcgcgg ttacctttgt
    11941 atccttgtac gcatcgtcca aggcggcacg atctgcaaca aaacgcgccg catgggcagc
    12001 atagattcgg aaattggcga cgtcgccatc gccgatgctt gcgtcgtgcc gccacccgtg
    12061 gatgaagacg acgacgtagt tcgatttgtt tccccttagt tgcttgacca gcgcctcgag
    12121 ttgaccctcc gcctgacggc gaaccggtat cgaccgttca cggatacatt cctcgccggg
    12181 atcacattcc tttcgcaggg catagggttt gccgtcctcc tggaattcaa taaatccgag
    12241 atcgtaactc aatgatttcg gcgcttcgtc gccttgcccg ggagcgaggt tgcgagccgg
    12301 tatgatgtgg tgctggatcg agcacgcaag cttgcgcgcg gagatgctgt tttggtcgat
    12361 tgccgtccac tcgttgttgt tcgcagccgc aagggcgtcc catcccgggg aaagtgcatc
    12421 ctggcaggac gcggaatcag cggcgagagc gcgggaggga atgtgccgat agctcggaaa
    12481 aaacagcagg ttggtcagga agacgagaac gaacgctgcg aacaccacca ggacgagccc
    12541 tttgagtatc ctgcccatcc atctgacccc gatcaggaag acgagatcga acgctgcaag
    12601 caccaccagg acgagtcctc tgaggatttt gcccgtccat ctgaccccga tagccagccc
    12661 cccagacaac ggccagcgcc gttgagacgg atctgcctcg gccatttgct ttcccccgag
    12721 attcgccgcc actcagcacg atcttgcacg ttgcatcggg ctgcgcttgg caaagcaatg
    12781 cgccggacaa attcgtcaca acaacggcgt cggagaaatt ttcgcgcgat ccagcgggct
    12841 ggcgatcata aagtgtattt tgtatgttat gcaatcaatc atattgtcga acagtcgcga
    12901 gcaatatgcg gcctgcgcgc cgcgctagcc ggtcttgcgc gcaagctcca tcgcggcggc
    12961 accgctacgg cccaccagcg ctgcgtactg tccgatcggc gcgggcttgc cgaggaaata
    13021 accctgtacc gcgtcacagc cctcgtcggc gaggaacccg agttgctcct gggtttccac
    13081 gccctcggcg acgatcgaca tttcgagacc gtggccgagg ccgatgacgg cccggacgat
    13141 cgccgccgat tgcggatttc gttcaagatt catcacgaag gcgcggtcga tcttgatttt
    13201 gtcgaacgga aacgcctgca gatagctcaa tgaggaatag ccgctgccga aatcatccat
    13261 cgagatccgc acgccgagcg ccttcagccg ccgcagcagc gccaggccgc gatcgaaatc
    13321 ctcgatcagc acgccttcgg tgatttcgag ctcaagccgg cccggcgaca ggccggtttc
    13381 gagaagtatc gagtgcacga ggctgaccag atcgccatgc atgaactgcg ccggcgacag
    13441 gttgaccgcg atctgcagcg gcttcgacca cgacgcggcc tcccggcagg cttcgcgcaa
    13501 gatccattcg cccatttcga cgatcaagct gctttcttcc gcgagcggga tgaaatcgcc
    13561 cggcggaacg aagccgcgga ccggatggag ccaccgcgcc agcgcctcga agccaatgac
    13621 ctcgctggag gcaaccgtct ggcccgaggc cgcctgcggc tgataatgca gcgacaactc
    13681 gccgttcttg atcgcgagcg agagatcctg gtgcagcacg cggcgatcgc gaatctgctg
    13741 gtccatctcg ggttcataga cgctgatcga gccgcgcgat ttcgccttgg cgcggaacaa
    13801 tgccgcgccg gcgttggcaa gcagcgatgc ggcgtcggcg ccgttgtggg ggaataccga
    13861 gatgccggtg gtgacgccga tgcggaccga tttgccgtcg atcgggaact cctgcgccag
    13921 cacttcggcg agctgttcgg ccaaagcctt gccggcggcg ggttgcttgc cgtcgatgat
    13981 caatccgaac tcgtcgcctg agagccgggc cacgacaccg ccgcgcgccg aggtctggat
    14041 acggcgtgac acctcgatca gcagcttgtc gccgaccgca tggccgaaca cgtcgttgat
    14101 ttccttcagc ccgtcgagat ccacggaaag taccgcgaac tcctcatcgg tgccttcgca
    14161 ggcctcgatc atctgcgcca gtgcctgcag gaacgccgcg cggttcggca gatccgtcag
    14221 gccgtcatga tacgccatat gagccatgcg cgactcggtc tggcggcggt cggtgacgtc
    14281 ctcgtgggtc ttgatcaggt attgcggctt gccggtgtcg tcgagcaccg tcatgcggcg
    14341 cgtcaggaac agccgcagcc cgtccttggt gctgatcggg tgctcttccg tgagcaggcc
    14401 gcgcttcttg atcgccgcct cgtcacgcgc gatgatcagc tttgcttctc tcggattgaa
    14461 gatatccgca gccgtcaagc cggtggcgtc ctcgcgacga cggttgagga tggcttcggc
    14521 gctgcgattg gcgagaaggt accgcccgtc gctgacgcgc tcgacgatca gcgagaccgg
    14581 gatgttgtcg accaccagtt cgagaaactt cttggtatct tcgagttcct tggaaagcga
    14641 ctggcgatcg gtgacgtcgt cgaacagcgc gatcaggaat tccggctgct tgttgtcgtc
    14701 gcgcacgatc acgcgggtac tggccagaac gcgcttcttc gaaccgcgct ggaccacgat
    14761 ttcatttctg aactggccat cggatgagtt gagcgccgac cggtctgccg ccgcgattcc
    14821 tgccgcggtc tcggcctgga aaatctcgtc ggcacgcttg ccaagaatgt ggtcgcggga
    14881 aaagcgcgag aaccgctcga acgcccggtt ggcaaagata taacggccgt cctcgatgct
    14941 cttggcggcg acgcataccg gaacattgtc gagcatcgat tccaggaact gcttggtcga
    15001 ggccaattgt cgcgacagct tgcgctgctc gctgcaatcc tcgtgtgtcg ccaccgaacc
    15061 gccgtttggc aatgcaaagt atttgaccag aaccgaccgt ccgccgggca gctcggtgat
    15121 gaggccttcc ggcgcgctgg cgtgcacata gaaatcgtcg acgctgacgt cgaggacgcc
    15181 gcgcttgagt tgcaacgcca gcaattccgg cccggtcatg tctttggcaa ggtccgaacg
    15241 cgccaggccg tagatctcga gatagcggtc gttgcagaac acgatccggc gtcgcggatc
    15301 cgtcatcacc acaccatgac tgaggttgtt cagcgcggaa cggacaaagg cattccggcg
    15361 caactccgag cgcttgacct tgcgcaacgc cgagagcacc cacaacgcga tggccgcaag
    15421 aaacgacgag acggcgatgc cggcaatcag catctcccaa atcgcattgg ggtcggctga
    15481 accgacgaag ccggatggcg aaaagtcgcc ggatgcgcca tgggcggatc cgaacggcgc
    15541 aacggctgcg atcaggcaca cgacggcctg cgcaggaatc aaattcttgc cgccagccag
    15601 ccagtccttg tcaaccatca atcacccgcg cattgctgcg ccgcagtgtc tggcttgacg
    15661 ggtttggatc gggtaaacgc ctacccgccc aagccaaaaa tgtaagtcaa attacggcaa
    15721 ttgccctgac atgattaatg cttcactaat ggcgacgagg ctcgagaaat atccggaaaa
    15781 tgaatccatc cgattattcg aaaagaccat cccagacaga gggatagagt aggaacagga
    15841 ttgatcccgg acacgcgccg actggtcgat gcggcgtcgg attggataga acacgaaaga
    15901 tcggatctga acccgcgaat ggataaagtt gagtgcgtcg tgatcggtgc gggggtgatc
    15961 ggtcttgccg tcgcgcgccg gctggcccag gccggccgcg aggtgatcat tctggaggcg
    16021 gccgaaggga tcggcaccgt gacctcctcg cgcaacagcg aggtgatcca cgccggcatc
    16081 tattatccgg ccggcagcct gatggcgcgg atgtgcgtca gcggcaaacg cgccctctat
    16141 cgatattgcc gggatcacgg cattccgcat cgtaattgcg gcaagctgat cgtcgcgacc
    16201 acccctaagg aagccacaaa actgcagtcg atcagggctc acgccgaggc caacggcgtg
    16261 ctcgacctgc aaacgctctc cggcgatgcc gcacgcacgc tggagccggc gctgaactgc
    16321 gatgccgctc tgctctcgcc ttccaccggc atcatcgaca gtcacgcctt catgctggcg
    16381 ctgcaaggcg atgccgaagc tgccggtgca gcctgtgcct ttcacacccc gttgctgcac
    16441 gccaaggcgc tcgcggaccg gatcgaactc gatgccggcg gcaacgcccc gatcagcctt
    16501 gagtgcaagc ttctcgtcaa tgccgcgggg ctttctgcgt ccgcggtcgc gcgcagcatc
    16561 aacggcatgc cgatcgacct gataccggcg gcctatctgg caaagggaaa ctatttcagt
    16621 tgcagcgcgc ggtcgccgtt ttcacgcctg atctatccgg tgcccgaacc cggcgggctc
    16681 ggcgtgcacc tcactttgga tatggcggga caggcccggt tcggtcccga cgtggaatgg
    16741 gtcgatagca tcgattacgc cgttgatccg gctcgggcgg aaaaatttta tccggcgatc
    16801 cggcgatact ggccgacgct cccggacggc gcgctgatgc cgagctattc ggggattcga
    16861 ccgaagatcg tgccgccggc ggtggcgacg caggatttcc tgattcaggg gccacgagat
    16921 cacggcgtcc ccggactgat caatctattc gggatcgagt ctccgggcct gacatcatcg
    16981 ctggcgatcg ctgatcatgt cggcgaactg gctgccatgt aagcccatcc cggagcggca
    17041 gaccggctcg atacctgacg ccgtcatgct tcgccaacgg gacgcgctac gcgcggaccc
    17101 gttggcgaag catgacagca gtatttggga agcctgtatt tgggaagcct gttaatagac
    17161 tggctggtgg gcagcctgtc agtgcagcgt ttcgctggcg gtatgtctca gccgttcgat
    17221 ctcgtccttg accatcaatt ttcggcgctt cagctcaaca atttccaggt cgtctgttga
    17281 aaggtgcacg agagcttcgt gcaattcgtt ttcgagaacc ttgtgtttcc gctccagttc
    17341 aacgagatgc gcctcaattg ccatacgaaa cctcctctgt gggattaaga ttcgatccgg
    17401 agggccgatt gtacacgagc cggcgaagct gtcgatgggt atcgaaattg gcgttctcat
    17461 atttgagcgt catatgtaac gaagcgtgag catggaaccg gtctggtgtg cagcgcgaaa
    17521 gcccgcggcg ccaccggagc caacttccga tttgagggca tgccggaaac ccctgattcc
    17581 agtgcgattt tgcttcaaac ttccggccgg cggcgaaaat aaggaagaac cgccaagcca
    17641 ccgccacacc agcttgcaga ccgccctcgc agggacgata ttcggtccac aggtttccac
    17701 aaccgttcac aacctcatcg cgtttttcag ctaacgtaca ccatgaccaa cgaagatgag
    17761 cgcgaactcg aatccgagct cgcacggctg cagcaggagc accgcgatct cgatgcggcg
    17821 atcgatgccc tgcatcagtc gcccgcgccg gatctgttgc ggctgcaacg attaaagaag
    17881 cgcaagctgc agttgcgcga ccgcatcgcc ttcatcgaag atcagatcac gcccgacatc
    17941 attgcgtagg tcttgtcagc gccgtggatt catatctcga tattcaagcg ccgctgatca
    18001 attcccgcag cacccccgtc acctgcatcg gattataggg cttcggcaga aaccgtccgc
    18061 cttcaggcag ttcggtttcg ctgacatgga cgtggccgga ggttgcgata atcctgatcg
    18121 gcggccagcg gcccctgacg gctcgcgcga gcttcaaccc gtccatggaa cccggcatct
    18181 ggatatcggt aaaaaccacg gtgatgtccc ggcgagcttc cagaatttcg atcgcttcgt
    18241 cagcgtttgc agcctcgacc acctcgaacc cggcggcttc gatcatatcg accgcgtcca
    18301 tgcgcaacag aaactcgtcc tcgacgatca gcaccacggg tcttttggtc tcaacgagac
    18361 ccatgcgtct cactccccta tgaacacgcg ttaagtcccc gattttgttt tggttccagc
    18421 attgatgata tctcgacaag ttctcgacag gtcgttgagc ctgcgctagc gtttttggcg
    18481 gggactacac cgaaggcgct tcatggcgac cgacctcagc gcggacatcg ctgccgtgca
    18541 gggcatcgcg gcggtcccgt gcattctcga agtggtgtgc cgatcgaccg gcatgggttt
    18601 tgcggccgtg gcgcgggtca ccgagcagcg ctgggtttgc cgcgcagtgc gccacgaaat
    18661 cgagttcggt ctcgtgcccg gcggagagct tgaggtcgag accaccctgt gccacgagat
    18721 ccggcagagg ctacgaggcc gtcgtcatcg acaacgtcgc cgaagacgcc gcctactgcg
    18781 gacatcacac cccggcgaag tacgggttcc agagctacat ctcgatgccc atcattctcg
    18841 ctgacgggac cttcttcggc accctgtgcg cgctcgatcc gcggccggcg cagctggata
    18901 cgccggagac gatcggcatg ttcaagctgt tcccggaatt gattgcgatc catctcgagg
    18961 cggtggatcg gctggccgcc agcgaggcca ggcttttgag cgagcgcgaa acttcggagc
    19021 ttgcgcgagc aattcatcgc cgtgctcggc cacgatctgc gcaatccgct cgcctcgatc
    19081 gccgcggggg acgcaaatgc tgaccaagga agcctcgagg gaagccgcgg gcgaaattct
    19141 cggattgatc cagaagagcg tagcgcggat gtcggcgctg atcgacaacg tgctggattt
    19201 cgcgtgcggc cggctcggca gcggaatagc gctgaagcgc tcgccccaat cattgcagcc
    19261 ggtcctggat cacgtgatcg cggagcttcg cgcaagctta cccgaacgga acatcgaaaa
    19321 gatgttcgaa aaaattcctg ccggggaact gggccgaggg ccttgactcc atgagaacaa
    19381 aataggaaca ttgcgcctgt ctccagccat cgggaatttc gccatgtccg aatcctcttc
    19441 acagtccgac gtcagccatt acgagcaggc ctgcgaccag gcgattgcga tgtgcgacgg
    19501 aaacatgcgc agcaccatca aggcgctgat catggcgaat gaatatctgg aaagtgaact
    19561 acgggaattg caggcggcag ttgagagcgg ctgcgcggcg gcaggattgc cgcacgcaag
    19621 ttacgacgcc gcctgatcgc ggaaccaaac ctggagcgag acaatggtcg atgtcatcta
    19681 ctacgtcgcg ctgccattcc tgcaggacga taccggcgca ccggtcgccg gggcggcgga
    19741 agagtgccag agttcgtcgg gcgcgttgcg gcgcgcggaa gtgttgtcga aggccgccgg
    19801 cagcgttggt gcggtcgcct tcagccgcac cggcgatccg atgatcggcg aattcagcga
    19861 cgcgaaattg ctgagaaaat tcggcgacgt gccggatgat ctgagcgggc tctgataaat
    19921 tcgcttccgg atcacccatc gcgcgcggcg tcatcgcaag tcgcgaaaga aggcccgcac
    19981 ttcgccggcg agcagttccg gctgttccag tgctgcgaaa tgtccaccct tgggcatgat
    20041 gctccagcgg cgaatgtcgg tgaacacgcg cgccgcggca ctgcgcggcg gcttgaggat
    20101 ttcgcgcggg aattgcgcgt agccggtggg caccacaatc gtatcgcccg cgggcaggat
    20161 cggcgaaccg tgaagacggg cgtaataagg ccagaacgac gagccgatcg ccctggtaaa
    20221 ccaatagagc gagatgtcgg cgagcatgcg gtcgcggccg atcgcgagct cgacgtcgcc
    20281 atcgcaatcc gaccaggcgc ggaatttctc cacaatccaa gccgcgaggc cggtgggcga
    20341 atcggtgagg ccgaaggcaa gcgtctgggg gcgtgtgccc tggatccatt gatagccgct
    20401 ctcttctcgg gtccagtgcg caagttcgcc gaggtaacgc cgctcctcct cggtcggatc
    20461 gggaaaggcg gagggatcgc ggcccgccgc catcatcagg ttgatgtgga tgccgatcat
    20521 ccggttcgca tgcgcatagc cgagtcggct tgagaccgca gctccccagt cgccgccttg
    20581 cgcgccgaag ctatgatagc cgagcacgtc atgcatgagg gcggcaacgc aatccgcgat
    20641 ctcgggaacg ccgaaccgcc tctggccggg ctggaaagaa aggccgtagc ccggcaggga
    20701 cggcgccacc acggtgaagg catcgcgcgc gtcgccgccg aaccgtgccg gatcggtgag
    20761 gcgcgggatg aactcgagaa actcgaacac cgagccgggc cagccgtgca gcaacagcag
    20821 cggcatcggg tcggggccga cgccgggcac atgcaaatag tgcaggtcga cgccgtcgag
    20881 cgggacgcgg aattgcggaa aggcgttcag cgcggcctcc tcggcccgcc agtcgaaatc
    20941 gctgcgccag tattcggcga gcccgcgaag atacgcgaca tcggtgccat aggcccatgc
    21001 atcaccgggc gcctggtcgg ggaaacgcgc gcgggccaga cggtcgcgga gatcggcgat
    21061 gtcggcatcc gcaatacgaa gcgtgaaggg ggtgggtttg acggtcatga tctgctcggc
    21121 cggtgggaca gagcgctcag tctctagaaa ccctcagccg ccgtccagcg gcggtgtcgg
    21181 cctgtcgggg gactgaatat gcatatcagg cttcatgccg ccgctgcgcc ttgcgcacat
    21241 acatcgcgct gtcggcctcc tccagcgctc tggccgcttc cgcatgcgtg ccgagaacag
    21301 cgacaccggc cgacgcgccc gcggcgacgg tgcggccgcg gaacacgaat gagagccggt
    21361 cgatggcttg ttccagcgcc gccgccttgg cgtgggcgtc ggtttcgctc agattccata
    21421 acagcagcgc gaactcgtcg ccgccgagtc gcccgatcac gtcggaggcg cgcacgtggc
    21481 gcagcagcgc cgccacaatg gccttgagca cctgatcgcc cgcagcatgc ccgaacgcat
    21541 cgttgatcgg cttgaggcga tcgacgtcga gcacgatcag tgcggcgctc gcatggtagc
    21601 gcttgatgta ggcgacagcg cgattgagct cgcgctcaaa gccgcgccgg ttcggaatat
    21661 ccagcaggaa atcggtatcc gccgaagcct ccaattcgtc aattcgcgcc agtgcctgcg
    21721 ccaactggac cttgagccgg cgaatcgtca gtttggcctc gttgggggcg accgccagcc
    21781 gcggcgccga cgcggaagcg ggtttcaccc gtctggcgcg ctgcggcgcc gatttgcgct
    21841 ccgccacgcc gcttttacgg cgtttcgccg tccccggagc ggtcgccttg gttttcttct
    21901 tcatgaacat ccttgagaag acttgccgga attcaccaag acaggatagt ccattccgga
    21961 ccccttgcca cgccttgccg aagcctgggc cgatcttata atccggccta tgcttttgga
    22021 ttcccgaaaa gaattgaccg tatgaccgcc ccgatcgcca tcatcatggg aagccagtcc
    22081 gactgggaga ccatgcgcca cgccgccgac acgctgaccg cgctcggcgt cgcctgcgag
    22141 aaacacatca tctccgcgca tcgcaccccg gaccggctgt tcgcctttgc caagggcgcc
    22201 aaggccaccg gcttcaagat cattatcgcc ggcgccggcg gtgccgccca tctgccgggc
    22261 atgaccgcgt cgctgaccga gcttccggtg ttcggcgttc ccatcgaatc cagggcgttg
    22321 tcgggcctcg attcgctcta ttccatggtc cagatgccgg ccggggttcc ggtcggaacg
    22381 ctggccatcg gcaagcccgg cgccatcaac gccgcgctgc tcgcggcaag cgtgctggcg
    22441 ctgggcgatc cggcactcgc cggcagactt gtcgcctggc gcaaggcgca gaccgaggcg
    22501 gtggccgagc gcccggaggg atctgcgtga cttcgacaaa tcaggtgaag ctgaagcccg
    22561 gcgacaccat cggaattctc ggcggcggac aattgggccg aatgctggca atggcggcgg
    22621 cgcggctcgg cttgcgctgc caggtgtttt cgccggatcc ggattcgccg gcgttcgatg
    22681 tcgtgctgaa cgcaacctgc gcggaatatg ccgatgtcga ggcgctggag ctgttcgcca
    22741 acgatgtcga cgtcatcacc tacgaattcg agaacgtgcc ggcagcctct gcgatgatcc
    22801 tggccgcccg gcgtccggtg ctgcccgacc gcgacgtgct ggaaaccacc caggatcgcc
    22861 tggtcgaaaa ggattttgtc aaacggctgg gcatcggcac cgccgattat gccgatgtgt
    22921 cgtcggcggc ggaactgcac gcggcgatcg ggaccatcgg ccttcccgcc gtgctcaaga
    22981 cccgccgctt cggctatgac ggcaagggcc aggcgattat ccgcgacggc gatgatccgg
    23041 gccaggtctg ggaggatctc ggcaccaaat ccgcgatcct tgaggccttc attccgttcg
    23101 agcgtgagat ttcggtgatc gccgcgcgct cggccagcgg cgatgtcgaa tgtttcgatg
    23161 tcaccgaaaa cgagcacagc gaccacatcc tgaaaatatc gcgcgcgccg gctgcgattt
    23221 ccgaagcgct ggcggctgag gcgcgctcgg tcgccgagcg catcgccaat gcgctcgatt
    23281 atgtcggcgt gctcgcggtc gaaatgttcg tggtgcaggc cgatgacggg ccgacggttc
    23341 tggtcaacga aattgcgccg cgggtgcaca attccgggca ctggacgctc gacggcgcgt
    23401 cgatctcgca gttcgagcag cacatccgcg ccattgccgg ctggccgctc ggcaagccgg
    23461 tccgtcatgg cccggtcgtc atgaccaatc tgatcgggga cgagatcaac ggctatgagc
    23521 agtggctgac ggtgccgggc gcgaccgtac acctctatgg caagggttcg ccgcgaccgg
    23581 gccgaaagat gggacacgtg acgcaggtcg gagcggcgcc atcaaaaact gcgtgaccgg
    23641 gatcgaaaag atctgatttg gctgtgcccg caggtggaca ttgccgcctt agtctgatac
    23701 atccgcgcgc tcagggcacg ggcacggcct tttgccgccc ctttgcttta ccataattca
    23761 aatccgatca atggaaagag gatgccgcgt gcaggttctc gttcgcgata acaatgtcga
    23821 tcaagccctc aaggcgctga agaagaagat gcagcgcgag ggtattttcc gcgagatgaa
    23881 gctccgcggc cattatgaaa agccctccga aaagaaggcc cgcgaaaagg ccgaggcggt
    23941 gcgccgcgcg cgcaagctcg cccgcaagaa gctgcagcgc gaaggtctgt tgccgatgaa
    24001 gccgaagccg gtgtttggcg ccggtccggg cgccgatcgc ggtggtgctg gcggccgtgg
    24061 cggtcccggc ggcgggccgc gcgggccgcg ctgatcttac ttatctgctg atttgattgc
    24121 gatgacgcgg gcctttgtgc ccgcgttgtt gtgttgaccg atgcaattga cgacacacca
    24181 agctaccaat gctgtgtaag cacgcgagtg ttccgcagat atggcccgtc cggtttccca
    24241 tggcttttcc ggagcttcgc gccttagccc gcgcgcgttg acggtcgtcg ctctgctcgc
    24301 gctctgcggc tgctcggtca atctgggatc gctgtcgcca gagctcgata aggacccagc
    24361 gccgaagccg gctccaaccg agacgagcac gggcgacgcc caggccgaca ccgcgcgagg
    24421 tcaggcgctg gcgcgatccg gaaagaccga agaggcgctc gccgagttcg acaaggcgat
    24481 cgcgctcgat ccccattgcg ccgaggccct gtacgatcgc ggcctgctct atcaggctga
    24541 aaagcagcat caactcgcga tcgacgattt cacctcggcg aatggcctga cgccgcagcg
    24601 ggccgaacca ttgctgggcc gcgcgatcag ctaccttgcg ctggaccgga tcagggaggc
    24661 cgccgccgat ctcgacgagg ccgtgcaggc cgacccgacc aacgcgcaga tctggaccac
    24721 ccgcgggctt gcctatgaac gcctcggcga caagaccagg gccgccgggt catatggccg
    24781 cgccgtcggc atccgcccca aagacgaagc cgcgcgcaac ggctttgcgc gcgtcggcgg
    24841 caaggccggc caaacctacg aaccgttcta ggttatttcg gcaacacggc gtgaaacacc
    24901 gatttcgccg ccgacagcac gtcttcggca acgttccggc gttccttcgg cagcggctcg
    24961 atcgcctcgg cacgcaggtc gagcggctgc gagaccggaa tatcggccgg cggcgtcggg
    25021 cggtgtggat cgtcgttccg cgcggcatcg tagggcggcc ttccctgcgg cgatcctgcc
    25081 gccggatcga acggttcgcg tggcggcgtc gagaccatga tcggcggcgg aagcggccgg
    25141 accgccggtg cgggcacggc acggggcgat tcgatcggcg ctgcgtacac gacgtggggc
    25201 gcgtcaggga tacgggccgc gtccggggcg cgggccactt ctggcgcacg cggcgacgtg
    25261 tcagccgtca ggccgcgcaa tcgctcgatc gccgcacgtg cgagttcgtt ggcatcgcgg
    25321 tgctcatcaa caggcgttgc gggcgccgct gcctcggccg gcgggaccgg atttggcgcg
    25381 gcggcgacga ccggcaccac agccggcgca gaaggttgcg gcgcggggac cgtcttggcg
    25441 accgtcttct cgcgcggcgc agcctggtgc ctccgcgtat ccgggggaat gcttgcggtc
    25501 tcggcgggct tgtcggcgga tttctccgcg cccgctcggt cgaggacggc cttttcggag
    25561 atacccttcg ccctgacgcc cggctcgggg atgttggcga catcggtgga ggtttcgggc
    25621 gtcttggcgt ctgtcttgtt ggcgtctgtc ttggcctcgg cctttacagc ctcgggcgtc
    25681 ttgggatcgg ccttcggctc ggcggtcgac accaccgcgg cgaccggtgc gtcaggcttg
    25741 ttgatgtaat gattgacgat gtaggccccg ataacggtcg ccaacagcga cggcaataca
    25801 tccagggtga atttcgagag gtatttcagc atggccagcc tcttcccgcg tccgttctga
    25861 tgcggaaact ttgaggcatt tttgaggggt tgactgtgac ggatcggacg gatcggtgga
    25921 aagccggcaa atggggcggg gttatggttt caattcgact tccaggaaca cgatctcggt
    25981 cgcggtctcg ttgagcacgt catgttccac cccggccttg cggaagtagg acttaccgag
    26041 gccaagctgc gccttggagc gttccccgcc gggcgccacg atggtcattt cgcccgcggt
    26101 cacgggaacg atgacataat ccatctcgtg aatgtgatga ccggtggcgc tgcccggcgc
    26161 cagccgccac tcggtgacgc ggacttccgg cgtatcgacc tgaatatctg atttggcgct
    26221 cagcattatc cttcactcca actctcacgg cgcaaaaacc aggaaaacga ataccgcaaa
    26281 caccaccatg tgaaccagcc cgaacaggat attggtgcgg ccggtgccga aggtgagcat
    26341 gctgaggatg aaggtcatcg ccatcagcac catggcctga gcgtcgagcc caagcaccag
    26401 ttccttgtcc agcgcgtagg ccgccaccgc gaccgccggg atcgtcagtc cgatggttgc
    26461 cagcgaagaa cccagcgcca gattgatgct cttctgcaga tcgttcttgc gcgcggcggc
    26521 gacggcggcg acgctttccg gcagcaatat caacagcgcc accaggacgc ccgcaaaagc
    26581 cggcggcgca ccgatccgcg cggtcgcgaa atcgaccacc agcgagaact ttttcgccag
    26641 cagcaccacc gccagcagcg agatcaggag cagcgccatg ctcaggccca gcaggcggtt
    26701 cgaaacatgc gtcccgccat ccgcggcgcc tgtggcttcg ctgacgaaat aactccgatg
    26761 ccggatggtc tgggtataga gaaacacccc gtaaagaatg atggtgacga cactgacaaa
    26821 accgagctgt cccgccgagt agatcggccc cggcgtggcc agcgtgtaat tgggcatgat
    26881 cagcgtgatg gtcgccatga cgatcaggac gctgagatag aggctggatc cggtgacctg
    26941 aacatcctgc tcgcggtagc gcagacctcc ggccaggatg cagatcccga ccaggccgtt
    27001 gcacacgatc atcaccacgg cgaacaccgt gtcgcgcgcc aatgtgggga cgggtttttc
    27061 accgagcatg atggtcgcga tcagcgcgac ttcgatgatg gtgaccgcca gggtcaacag
    27121 cagggtgccg tagggctcgc cgatcctctc ggcgatcaac tcggcatggt gcacggccgc
    27181 gaacacggtg ccgaacagaa tgatcaacaa taccaccgca aacccaagcc cggcggccga
    27241 gggcgtgaag gtgaggccga gcgcggtggc gccagcgaaa aacaggaccg ccagcacggg
    27301 aaagatccac gccgatcgcg gcatcgggcc gtacgcgctc atgctgtcat atcctctcgg
    27361 cgtggacacc gattatgcac caagcgacag ccgcggtcag ccatttgggc cgccaatgaa
    27421 ctgtcgtgcc gtcgagatcg caccatgatt gccgagatcg tcgtggcctc ccaggggaaa
    27481 ccgcacgaat tgtttcggcc ccggggcgag cccgaacaga cgctcgccga actcaatcgg
    27541 aatggttaga tcgcgttcgc catgcatgac caggagcgga gcggtgacgc gcgccatgcg
    27601 ctgatcgctg tgaaaccgat cccgcaacag ccagcgcacc ggcagaaccg gaaaatgccg
    27661 ggcggcaaca tctaccatcg aggtataggg agcttccagg atcagctttc caaccggctg
    27721 gtcggctgca acagcgaccg cggcgccggt gccgagcgaa aagccccaca gcacgattcg
    27781 atcgggtggg tatcgcgcgg ccgtgaaggc gtaagccgca gcggcgtctt gcagcagtcc
    27841 ctgctcgctg ggctggccgc tggagccggc atagccccga taggacaacg cgacgagccc
    27901 ggtcccatca gcggcgatgg cccgaaagcg gccgacgttg cccgcgagga aatctccatt
    27961 gccgggaaaa tagatcacga ccgcatggcc cggctgtgcc ggaacgtgcc aaatgatgac
    28021 cttctcgcca tcggctgtgt tcagaacatg ctcttcggct tcgggaaagc cggcggcttc
    28081 gggggccgtg cgtagcgttt gcggtatcgg aaagacgaat tcccgttgct tgaagaaaac
    28141 gacggctaga ccgccgagat agccgacgat tgcgagaatg acgagccatt tcaggacgat
    28201 catgatttcc cgtgaacagc ctcacagcgc cttgacgata ttctcggtca tcttcttggc
    28261 atcgccgagc agcatcatgg tgttgtcgcg gtagaacagg ggattgtcga tgccggcata
    28321 gcccgacgcc agcgagcgct tgatgaacat cacggtgccg gccttccaga cctgcagcac
    28381 cggcatgccg tagatcggcg atttcgaatc gtcttcggcg gcgggattgg taacgtcatt
    28441 ggcgccgatc acgaaggcga cgtcggcttg agcgaattcc gaattgatgt cctcgagttc
    28501 gaacacctcg tcgtagggca cattggcctc ggcgagcagc acgttcatgt ggcccggcat
    28561 gcggccggcc accgggtgga tcgcgtactt gacctcgacg ccgtccttct tgagctggtc
    28621 ggccatttcg cgcagcgcgt gctgcgcctg cgccaccgcc atgccgtagc cgggcacgat
    28681 gatcaccttc tgcgcattct tcatgatgaa ggcggagtcg tcggcggagc caagtttcac
    28741 gggccggact tcgccacccg cgccggcgcc cgcggcggcc gtctcgccgc caaaaccgcc
    28801 gagaattacc gagatgaagg aacggttcat cgcgtggcac atgatgtagg acaggatcgc
    28861 gccggaggag ccgaccagcg cgccggtgat gatcagcgcc gagttgccga gtgtgaagcc
    28921 gatgccggcg gcggcccagc cggaatagga gttcagcatc gagatcacga ccggcatgtc
    28981 ggcgccgccg atcgggatga tcaggagcgc gccgagcgcc agcgcgatga tcgtgatcag
    29041 ccagaagtcg agcgcactgc ccgtcagcac gaggccgaca atgaacacca ccagcgcaat
    29101 cgccaatgcg acattgatga cgtgacgtgc gggcagaata atcggcgcac cgctcatgcg
    29161 ccccgacagt tttgcgaacg cgatcaccga gcccgtaaac gtcagcgcgc cgatggcgac
    29221 gccgagcgac atttcgacca ggctggcgcc gtggatgttg ccgggcgttc cgatgtcgaa
    29281 ggcttcgggc gcgtagaacg caccggccgc gaccagcacc gcggccatac cgaccagcga
    29341 gtg
//
LOCUS       DATNOI010000473         4594 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2533718,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000473 DATNOI010000000
VERSION     DATNOI010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2533718"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="VN968_18875"
     CDS             complement(<1..167)
                     /locus_tag="VN968_18875"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HXL31335.1"
                     /translation="MSSKNGNTKFTNATGAPVADNTNIMTAGRRGPALLQDIWLIEKL
                     AHFDREVIPERR"
     gene            complement(285..944)
                     /locus_tag="VN968_18880"
     CDS             complement(285..944)
                     /locus_tag="VN968_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXL31336.1"
                     /translation="MTIDLTRNTDLAGTTIPDSKLAREITELVRDTESPLLFHHSSRV
                     YYWGALAGKRRRLKFDPELLYAGAMFHDMGLTHQHSSQTERFEVDSANAARDFLRSHR
                     IAQRDIETVWTAIALHTTPGIPPHMHPVVALVTAGVEMDVLGLAYSEYSDAEREAVVH
                     AHPRPPRFKQEIIQAFYDGNKHKPETTFGNVNADVLADKDPHFARVNFCSVIRGSAWR
                     G"
     gene            complement(979..3177)
                     /locus_tag="VN968_18885"
     CDS             complement(979..3177)
                     /locus_tag="VN968_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXL31337.1"
                     /translation="MEMNSPVGPNALDAEGVVGKPLDRIDGRLKVTGGARYAYEMQQN
                     TLYGFVVEASIGKGKIKSIDTRAAENAPGVILVLTHRNAPEQGVGNHREAHPVLSGPE
                     VTRYGQPVAFVVAESFEQARAAAYLVDVKYDRSGGKYALRANLDQARVPHQSDGAAAD
                     SAVGDFARAFASAPVQLDVTYTTPLQSHAMMEPHATLAMWEGDKLILHTANQMLNQGQ
                     KVIATTLKIPVENVRLISPFIGGGFGGKLWVNADAILAAIASRQLQRPVKIALTRQQI
                     FHVTTHRSDTIQRLRLGTDRDGRILAIGHDVFSGNLPSEQTYEAAALQTRTLYAGPNR
                     LTRHRLAPLDIPVASSMRAPGEAVGLLALECAMDELAEKLNLDPIELRLRNEPSEDPE
                     KHIPYSSRHLIACLQAGARRFGWDKRNARPGQVRDGRWLVGMGMAAATRGNPLQLSKA
                     NVRLGPDGIATVKMAMTDIGTGTYTILAQIAAEMLGLPVERVRVELGDTSFPEAAGSG
                     GSWGAGSSGSALFEACNSLREKLARSAGMDPATARFADGSISSGEQSRKLTELVGAGM
                     DADGEIQPGRNNKDFSQQSYGAHFAEVGVDADTGEVRLRRMLGVFTAGRVLNAKTARS
                     QAIGGMVFGLGSALEEGMTLDPRFGYFVNRDLAEYLVPVHADIPAIEAVFLGELDDRS
                     NPLKSKGIGELGICGAGASIANAVYNACGARIRDYPITLDKLLSKLPVQA"
     gene            complement(3189..4169)
                     /locus_tag="VN968_18890"
     CDS             complement(3189..4169)
                     /locus_tag="VN968_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXL31338.1"
                     /translation="MKAFTYQRADTAASAATAAVKPGAKIIAGGTNLLDLMKLQVETP
                     SALIDINRLPLDKIEDTSDGGLRVGALVRNSDLAADSRVRRRYGVLSRALLAGASAQL
                     RNKATTGGNLLQRTRCYYFYDITKPCNKRSPGSGCAALAGFNRIHAILGGSDQCIATH
                     PSDMAVAMRALDAKVETVNPEGEARVVPIAEFHRLPGKTPEIETSLKPGEIITAVTLP
                     PPPPGVQVYRKVRDRASYAFALVSVAAIVDSAQGKIRSARLAFGGLAQKPWRVAQAEQ
                     KLAGAPANTASFNTAANAVLEGARGYGGNDFKIPLARRTLHSVLAEITRT"
     gene            complement(4166..>4594)
                     /locus_tag="VN968_18895"
     CDS             complement(4166..>4594)
                     /locus_tag="VN968_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXL31339.1"
                     /translation="EHLALTGSKKGCDHGQCGACTVLIEGRRINSCLTLAVMHDGESV
                     TTIEGLAEGDNLHPLQTAFVEHDGFQCGYCTSGQICSAAGMLAENRQGMPSYVTEDLT
                     QPAAELTDAEIRERMSGNICRCAAYPNIVAAIKQAAGLPT"
BASE COUNT          776 a         1414 c         1483 g          921 t
ORIGIN      
        1 cgccgctccg ggatcacctc gcggtcgaaa tgcgccagct tctcgatcag ccagatatcc
       61 tggagcagcg ccgggccgcg ccggcctgcg gtcatgatgt tggtgttgtc ggcaaccggg
      121 gctccggtcg cgttcgtaaa cttggtattg ccgttcttgc tggacatgat cagtcctccg
      181 ttgggttgga ttctgttggg ttaggtcgcg ggtgccgaca tcggccctgc tgttcggccg
      241 atgacagctt cagtgctgtg ttctccagga caggattagt gggctcatcc gcgccaggcg
      301 gatcctcgga tgacgctgca gaaattgacg cgggcgaagt gcggatcctt gtcggcgagc
      361 acgtcagcgt tgacgttgcc gaaggtcgtc tccggcttgt gcttattgcc gtcgtagaag
      421 gcctggatga tctcctgctt gaaacgcggc ggacgcggat gtgcatgcac caccgcctcg
      481 cgctccgcat cgctgtattc ggaataagcc agcccaagga cgtccatttc gacgcccgcc
      541 gtcaccaggg ctacgaccgg gtgcatgtgc ggggggatgc ccggcgtcgt gtgcaaggcg
      601 atcgcggtcc agaccgtttc gatatcccgt tgcgcgatgc ggtggcttcg gaggaagtca
      661 cgcgcggcat tcgcgctgtc gacctcgaag cgctcggttt ggctgctgtg ctgatgggtc
      721 aaccccatgt cgtggaacat ggcgcccgca tagagcagtt cgggatcgaa tttgagccgg
      781 cgacgcttgc ccgccagcgc gccccagtag tagacgcggc tcgaatggtg aaacagcaaa
      841 ggcgattcgg tgtcccgcac cagctcggtt atttcgcgcg cgagcttgct gtcgggaatt
      901 gtggtccccg caagatcggt attcctggta agatcgatgg tcattacatc ctcctgctgg
      961 ttgatcttgc gttcctgatc acgcctgcac cggcaatttg gaaaggagct tgtccagggt
     1021 gatcggatag tcgcggatgc gggcgccaca ggcgttgtag accgcattcg caatcgaggc
     1081 gccggcgccg cagatgccga gctcgccgat gcccttgctc ttgagcggat tggatcggtc
     1141 gtcgagctcg ccgagaaaaa ccgcttcgat cgcgggaata tcggcgtgca ccggcacgag
     1201 gtattcggcg aggtcccgat tgacgaaata gccgaagcgg ggatcaagcg tcatcccttc
     1261 ctccaacgca gagcccaaac cgaacaccat tccgccgatc gcctgggagc gcgccgtctt
     1321 cgcattcagg actcgcccgg ccgtgaacac gccaagcatg cgccgcaggc gtacttcacc
     1381 ggtatcggcg tcaacgccca cttcggcgaa gtgcgcgcca taggattgct gcgagaaatc
     1441 cttgttgttg cggcccggtt ggatttcgcc gtcggcgtcc attccagcgc cgaccagctc
     1501 cgtcagcttt ctggactgtt ctccggatga gatgctgccg tcggcgaacc gtgcggttgc
     1561 cggatccatg ccggcgcttc gcgccagctt ctcacgcaac gaattgcagg cttcgaacag
     1621 cgccgagcct gaactgcctg cgccccacga tccacccgaa cccgcggcct ccggaaagct
     1681 ggtatcgccg agttcgaccc ggacccgttc aactggcagc ccgagcatct cggcggcgat
     1741 ctgcgcgagg attgtataag tcccggtgcc gatgtcggtc attgccattt tgactgtcgc
     1801 gataccatcg ggacccaatc ggacattggc cttggagagc tgaagcgggt tgccgcgcgt
     1861 cgccgcggcc atgcccatgc ccaccagcca gcgcccgtcg cggacctggc ccggcctggc
     1921 gttgcgcttg tcccatccga accggcgcgc acctgcctgc aggcaggcga tcaaatgacg
     1981 gctggagtag ggaatatgct tctccggatc ctcgctcggc tcgttgcgca gccgcagttc
     2041 gatcgggtcg aggttgagct tctcggccaa ctcatccatc gcgcattcaa gcgccagcaa
     2101 cccgacggct tcgccgggcg cgcgcatgga cgaggcaacc ggaatgtcca gcggcgccag
     2161 gcgatgtcgc gtaagccggt ttggtccggc gtagagcgtg cgggtctgca gtgccgcggc
     2221 ctcataggtt tgctcgctcg gtagattgcc cgagaacaca tcgtggccga tcgcaagaat
     2281 tcggccgtcg cgatcagtac ccaggcgaag gcgctggatg gtatccgagc ggtgtgtcgt
     2341 gacgtgaaag atctgctgtc gcgtcagcgc gattttcacc ggccgctgga gctgccgcga
     2401 cgcaatcgcc gccagtatcg catcggcatt gacccagagc ttgccgccga aaccgccgcc
     2461 gatgaagggg ctgatcagcc gcacattctc gaccggaatt ttcaaggtgg tcgcgatgac
     2521 cttctgtccc tgattgagca tctgatttgc ggtgtgaagg atcagcttgt cgccctccca
     2581 catcgcgagc gtcgcgtggg gctccatcat ggcgtggctt tgcaagggtg tggtgtaggt
     2641 cacgtcgagc tgaacgggcg cggacgcgaa tgcgcgcgcg aagtcaccca cggcactatc
     2701 ggccgcggcc ccatcgcttt gatgtggaac gcgcgcctga tcgaggttag cgcgaagtgc
     2761 gtattttcca cccgaacgat cgtatttaac gtcgaccagg tacgccgcag ccctggcttg
     2821 ctcaaaactt tccgcgacca caaaggcaac cggctggccg tagcgcgtca cttcggggcc
     2881 ggaaagaacc ggatgcgcct cgcggtgatt gccgacgcct tgctcgggcg cgttgcgatg
     2941 ggtcagaacg aggatgacgc ccggcgcgtt ctccgcagca cgggtatcga tcgatttgat
     3001 ctttcccttt ccgatcgaag cctcgaccac aaagccgtac agcgtgttct gctgcatctc
     3061 ataggcgtag cgcgcgccgc cggtgacttt cagtcgtccg tcgatgcggt cgagcggctt
     3121 gccgacgacg ccctcggcat caagcgcatt cggcccaacg ggtgaattca tttccatgag
     3181 tgcgctcctc aggtccgggt aatttctgcg agtacgctgt gcagggttcg ccgtgccagc
     3241 gggatcttga aatcgttgcc gccgtagcca cgggcgccct cgagcacggc atttgccgcg
     3301 gtattgaagc tggctgtgtt ggcgggggcg ccggccaatt tctgctcggc ctgcgctaca
     3361 cgccagggct tttgcgcgag accgccgaac gcgagccgcg ccgaccggat ttttccctgc
     3421 gccgaatcga cgatcgcggc gaccgaaacc aaggcaaagg cgtaggaggc gcggtcgcgt
     3481 accttgcgat agacctgcac gcccggcgga ggcggcggca gcgtcaccgc cgtaatgatt
     3541 tcgcccggtt tgagggaggt ctcgatctcc ggggtcttgc ctggaagacg atgaaactcg
     3601 gcgatcggaa cgacgcgggc ctcgccctct gggttgaccg tctccacctt ggcgtcgagc
     3661 gcccgcatcg cgactgccat atcggagggg tgcgtggcga tgcactggtc gctgccaccg
     3721 aggatggcgt gaatgcggtt gaagccggcg agtgccgcgc agccggaacc ggggctgcgc
     3781 ttgttgcagg gctttgtgat gtcatagaag taatagcagc gtgtccgctg cagcaaattg
     3841 ccgccggtgg tggccttgtt gcgcaactgc gcgcttgcgc ccgcgagcaa tgcacggctt
     3901 agcacgccgt aacgccgccg cacgcgcgag tcggcggcaa gatcgctgtt tcgaaccagc
     3961 gcgccgacac gcaagccgcc atccgaggta tcttcgatct tgtcgagtgg caggcggttg
     4021 atatcgatca gcgccgatgg cgtctcgacc tgcaacttca tcagatcgag caaattggtg
     4081 ccaccggcaa tgattttcgc gccgggtttt acggcagcgg tggcggcgct ggcggcggta
     4141 tcggcacgtt gataggtgaa cgctttcatg tcggcaatcc cgcagcttgc ttgatggcgg
     4201 ccacgatgtt cggataggcc gcgcagcggc agatgttgcc gctcatccgt tcgcgtattt
     4261 cggcatcggt gagttcggcg gccggctgcg tcaggtcctc ggtgacatag ctcggcatgc
     4321 cctgtcggtt ctcggccagc attccggcgg cggaacagat ctggcccgag gtgcaatagc
     4381 cgcactgaaa accgtcgtgc tcgacgaagg ccgtctgcaa cgggtgcaga ttgtcacctt
     4441 cggcaagacc ttcgatggtg gtaacggatt ctccgtcatg catcaccgcg agcgtcaggc
     4501 aggagttgat gcggcgccct tcgatcaaca ccgtgcaggc gccgcattga ccgtgatcgc
     4561 agcctttctt ggagcccgtc agcgcgagat gctc
//
LOCUS       DATNOI010000474         6642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2164249,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000474 DATNOI010000000
VERSION     DATNOI010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6642
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2164249"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="VN968_18900"
     CDS             <1..1265
                     /locus_tag="VN968_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HXL31340.1"
                     /translation="TAPLIAAGCIMMRKCHLNTCPVGVATQDPVLRKRFTGQPEHVIN
                     YFFFVAEEVREIMAALGYRTFNEMVGQTQMLDQSSLVAHWKAKGLDFSKLFVRQKELP
                     GQKIYHAEPQNHHLEAVLDRKLIEKAQAALDRGAPVKIDMEINNTDRSAGAMLSGTVA
                     KIYGHAGLPHDTIQVGFKGTAGQSFGAWLARGITFDLEGEGNDYVGKGLSGGRIIVKP
                     PRHSGIVPEESIIVGNTVLYGAIEGECYFRGIAGERFAVRNSGAIAVVEGAGDHCCEY
                     MTGGIVVVLGKTGRNFAAGMSGGIAYVLDEAGDFEKLCNMAMVELEPVLSEEMINENA
                     YHASGDLEAHGKVDVFGNLLESDIERLHILITRHAKLTGSTRAADILADWKTWLPKFR
                     KVMPVEYRRALKELKANADAEPKIAIGA"
     gene            1412..2869
                     /locus_tag="VN968_18905"
     CDS             1412..2869
                     /locus_tag="VN968_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HXL31341.1"
                     /translation="MGKITGFLEIERRDRKYEPVAERVKSFREFVIPLSEKDTRDQAA
                     RCMNCGIPYCHGTGSVAPGAPGCPVNNQIPDFNDLVYQNNWEEASRNLHSTNNFPEFT
                     GRICPAPCEASCTLNIDDNPVTIKTIECAIVDRAWDNGWVKPEIPTSKTGKKIAVIGS
                     GPAGMACAQQLARAGHDVHLFERWAKAGGLLRYGIPDFKMEKHHVDRRVAQMEAEGVT
                     FHYGAHVGGSSPGAIDPRELLNQYDALALTGGAEAPRDLPIPGRELDGIHFAMDFLPQ
                     QNRRVSDEPLGDAPDILAGGKHVVVIGGGDTGSDCIGTSFRQGARSVTQLEIMPAPPE
                     RENKALTWPNWPLKMRTSSSQAEGAKREYAVLTEKFSGDDGKVAKLHCVQVDDKFKPV
                     PGSEFELEAQLVLLAMGFVHPVHEGLLKTLGVDLDPRGNVRANTADYQTSLPKVFSAG
                     DMRRGQSLVVWAIREGRLCARAIDQFLMGSTNLPR"
     gene            3326..3531
                     /gene="gltD"
                     /locus_tag="VN968_18910"
                     /pseudo
     CDS             3326..3531
                     /gene="gltD"
                     /locus_tag="VN968_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022054.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
     gene            complement(3565..4515)
                     /gene="htpX"
                     /locus_tag="VN968_18915"
     CDS             complement(3565..4515)
                     /gene="htpX"
                     /locus_tag="VN968_18915"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HXL31342.1"
                     /translation="MNYLRTAILLAGLTALFMGVGYLIGGASGATIALVVAAATNLFA
                     YWNSDRMVLSMYGAHEVDQQSAPELVSLVAALAGRAGLPMPRVFLIDNPQPNAFATGR
                     NPQNAAVAVTTGLMQSLSREELGGVIAHELAHVKNHDTLLMTITATIAGAISILAQFG
                     MFFGGHRDNNSGPGIVGSIAMMILAPLGAMLVQMAISRTREYAADNLGARIVGQPMWL
                     ASALVKIENAAHEIPNWEAERNPATAHMFIINPLTGHGVDSLFSTHPSTENRIAELQR
                     LAAELGARGGAAPSTPPQSAPRRGPWGADRNRTAPRGPWG"
     gene            complement(4695..5144)
                     /locus_tag="VN968_18920"
     CDS             complement(4695..5144)
                     /locus_tag="VN968_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL31343.1"
                     /translation="MDAVTPMGSGTAETHSHIVRPDSMAWQKTRFPGCEAKTLLFDRT
                     TGLMTALMRFAPGAVLPDHEHVNIEQTYVLEGSLVDKEGPAKGIECKAGEFVWREQGS
                     RHVAWCPQGGLMLAIFQVPNKFFEADGRVVDAAGQDWDEAWGHTRKA"
     gene            5344..6357
                     /locus_tag="VN968_18925"
     CDS             5344..6357
                     /locus_tag="VN968_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31344.1"
                     /translation="MRKILAAAVLSAISASLMSAAPAQAQTPIKIMVGGIDKQIYLPA
                     KLAAQLGFFREQGLDVELFNSTSGSQAATALLAREVQGVVGFYDHTIDLQSKGKFITN
                     VVQFGVAPGEVVLVKSADADKLKDPANWKGQALGVTGLGSATDFLTRALAAKAGLKVQ
                     DYTLVPVGAGDTFLAAMQQGKIVSGMTTEPTVARAIKAGTAKVGIDLRTPEATRAALG
                     GDYPAACLYMDRGWMEANKETAQKVVNVFVKTLKWMNSHSPEEIAAQMPKDYYAGELD
                     LYVQGLREGRAQYSPDGMMPQGAPESVLKVLSSFSPNVQGKTIDLSKTYTTEFVIKAN
                     ATH"
     gene            6467..>6642
                     /locus_tag="VN968_18930"
     CDS             6467..>6642
                     /locus_tag="VN968_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL31345.1"
                     /translation="MDASLPTAVALKHVARRFVTPAGEILSALEDFDLSIAPGEFFAI
                     VGPTGCGKSTTLGL"
BASE COUNT         1248 a         2159 c         2112 g         1123 t
ORIGIN      
        1 ccaccgcgcc gctgatcgcg gccggctgca tcatgatgcg caaatgccat ctcaacacct
       61 gtccggtcgg cgtcgccacc caggacccgg tgctgcgcaa gcgcttcacc ggccagcccg
      121 agcacgtcat caactatttc ttcttcgtcg ccgaggaagt ccgcgagatc atggccgcgc
      181 tcggctaccg caccttcaac gagatggtcg gccagaccca gatgctcgac cagtcgagcc
      241 tggtggcgca ctggaaggcc aaggggctcg acttctcaaa actgttcgtg cgccagaagg
      301 agctgcccgg ccagaagatc tatcacgccg agccgcagaa ccatcatctg gaagccgtgc
      361 tcgaccgcaa gctgatcgag aaggcgcaag cagcgctcga ccgcggcgcg ccggtcaaga
      421 tcgacatgga gatcaacaac accgaccgca gcgccggggc gatgctgtcg ggcacggttg
      481 cgaaaatcta tggccatgcc gggttgccgc acgacaccat ccaggtcggc ttcaagggca
      541 ccgcgggtca gtcgttcggc gcctggctcg cgcgcggcat caccttcgat ctcgaaggcg
      601 aaggcaacga ctatgtcggc aagggcctgt ccggcggccg tatcatcgtc aagccgccgc
      661 gccattccgg catcgtgccc gaggaatcca tcatcgtcgg caacaccgtg ttgtatggcg
      721 cgatcgaggg cgaatgctat ttccgcggca tcgccggcga acggtttgcg gtgcgtaact
      781 ccggcgcgat cgcggtggtc gaaggcgccg gcgaccattg ttgcgaatac atgaccggcg
      841 gcatcgtcgt ggtgctcggc aagaccgggc gcaacttcgc ggccggcatg tccggcggca
      901 tcgcctatgt gctggacgag gcgggcgact tcgaaaagct ctgcaacatg gcgatggtcg
      961 agctggagcc ggtgctctcg gaagagatga tcaacgagaa cgcctatcac gccagcggcg
     1021 atctggaagc gcacggcaag gttgacgtgt tcggcaatct tctggagtcc gatatcgagc
     1081 ggctgcatat cctgatcacg cgccacgcca agctgaccgg atcgacgcgc gccgccgaca
     1141 ttctggccga ctggaagacg tggctgccga aattccgcaa ggtgatgccg gtggaatacc
     1201 gccgcgcgct gaaggaactg aaggcgaacg cggacgccga accgaaaata gcaatcgggg
     1261 cgtagaggta agcgtcatcc tgaggtgcga gccgccttcg gcgagcctcg aaggatgacg
     1321 agggaaggtg tttgtggctc atccttcgag acgcggcgaa gacgccgctc ctcaggatga
     1381 cggataagag ggttcagggg cttcagcttt aatgggcaag attacaggtt ttctggagat
     1441 cgagcggcgc gatcgcaaat atgagccggt cgccgagcgc gtgaagagct ttcgtgagtt
     1501 cgtcatcccg ctaagcgaga aggatacccg cgaccaggcg gcgcgctgca tgaattgcgg
     1561 gatcccctat tgccacggca ccggctcggt ggcgccgggc gcgccgggct gcccggtcaa
     1621 caaccagatt ccggatttca acgatctggt ctatcagaac aactgggaag aagcctcgcg
     1681 caacctgcac tcgaccaaca atttcccgga attcaccggg cggatttgcc ccgcgccgtg
     1741 cgaagcttcc tgcacgctca acatcgacga caaccccgtc accatcaaga cgatcgaatg
     1801 cgccatcgtc gatcgcgcct gggacaacgg ctgggtcaag ccggaaatcc cgaccagcaa
     1861 gaccggcaag aagatcgcgg tgatcggctc gggcccggcg gggatggcct gcgcgcagca
     1921 gctcgcgcgt gccggccacg acgtgcatct gttcgagcgc tgggccaagg ccggcgggct
     1981 gttgcggtac ggcattcccg atttcaagat ggaaaagcat cacgtcgacc gccgcgtcgc
     2041 gcagatggag gccgagggcg tcacctttca ctacggtgcc catgtcggcg gcagttcacc
     2101 cggcgcgatc gatccgcggg agctgctcaa ccaatatgac gcgctggcgc tgaccggcgg
     2161 cgccgaagcc ccgcgcgacc tgccgatccc cggccgcgag ctcgacggca ttcacttcgc
     2221 gatggatttc ctcccgcagc aaaatcgccg cgtctccgat gagccgctcg gcgacgcccc
     2281 ggacatcctc gccggcggca agcatgtcgt ggtgatcggc ggcggcgata ccggctcgga
     2341 ctgcatcggc acgtcgttcc ggcagggcgc cagatcagtg acgcagctcg agatcatgcc
     2401 ggcgccgccg gagcgcgaga acaaggccct gacctggccg aactggccgt taaagatgcg
     2461 gacctcctcg agccaggccg aaggcgccaa gcgcgaatac gccgtgctga cggaaaaatt
     2521 ttccggcgac gacggcaagg tcgcaaaact gcattgcgtc caggtcgacg acaagttcaa
     2581 gccggtgccg ggaagcgaat tcgagctcga ggcgcagctg gtgctgctgg cgatgggctt
     2641 tgtgcacccg gtgcatgagg gcctgttgaa gacgctcggc gtcgatctcg acccgcgcgg
     2701 caatgtgcga gccaataccg ccgactacca gacctcgctg ccaaaagtgt tttccgccgg
     2761 cgacatgcgc cgcggccagt cgctcgtcgt ctgggcgatc cgcgagggcc gcctctgcgc
     2821 ccgcgccatc gatcagttcc tgatgggatc gacgaacctg ccgcggtgac gcctcgtcgt
     2881 cccgcctggt gcgcaattgc gcacgcggcg cggcgacccc gtattccagg cggctgaaga
     2941 gacgcccctc gggctcgccg aattaatgtt tctgcagtcg actcccggac ggatggggaa
     3001 tctaatgacg ccatcaccgt ccctcttcgg cttgtaccgg tgactgagag ttttgcgctc
     3061 ccgcctgatc cagggtggaa atgcaaatga ccgattaccc agctgcaatc ccactgctcg
     3121 caattcagca tctccactgc ccgagatgcc gaacacgcat gacgcttacg cacatcctgc
     3181 cggggcctac cggattcgaa cttcgcacgt tcgactgctc caaatgcgat cacgtcgaac
     3241 aagtcgcgat acctttagat ccgatggaat ccggtgcggt tggttggttc actggcgaac
     3301 tacggccgcc caagtaactg gaacgctagt aagcgccctc ggcgtcggtc tcgatccgcg
     3361 cggaaatctc cgcgccaaca ccgcggacca ccagacctcg cggccgaacg tattttcagc
     3421 cggcgacatg cgccgcggcc agtcgctcgt cgtgtggacg atccgggaag gcccctctgc
     3481 gcccgcgccc tcgatcagtc tctgatggga acggcgaatc cgccgcgcta gcccccttca
     3541 ggtcgctgac gaactccctc tttttcagcc ccagggcccg cgaggagcgg tgcggttgcg
     3601 atcggcgccc caggggccgc gtcgaggagc cgactgcgga ggtgttgatg gcgctgcgcc
     3661 gccgcgcgca ccgagttcgg cagcgagccg ctgcagttcg gcaatccggt tctcggttga
     3721 cggatgcgtc gaaaacaggc tgtcgacgcc atgacccgtc agcgggttga tgatgaacat
     3781 atgcgccgtc gccgggttgc gttccgcctc ccagttcggg atctcgtgcg cggcgttctc
     3841 gatcttgacc agcgcggacg ccagccacat cggctggccg acgatgcgcg cgccgagatt
     3901 gtcggcggcg tattcgcgcg tccggctgat cgccatctgc accagcatgg cacccagcgg
     3961 cgccaggatc atcatcgcga tcgagccgac gatacccggg ccgctgttgt tatcgcgatg
     4021 accgccgaag aacattccga actgggccag tatcgagatc gcacccgcga tcgtcgcggt
     4081 gatggtcatc agcagcgtgt cgtgattttt cacatgcgcg agctcatggg cgatcacccc
     4141 gccgagttcc tcgcggctca gcgactgcat cagccccgtg gttacggcga ccgcggcatt
     4201 ctgcggattg cggccggtgg cgaacgcatt gggctgcgga ttgtcgatca agaacacccg
     4261 cggcatgggc agccctgccc gtcccgccag tgcggcgacg aggcttacga gttcgggcgc
     4321 actctgctga tcgacctcat gggcgccgta catggacagc accatgcgat ccgagttcca
     4381 gtaggcgaac agattggtcg cggcagcgac cacgagcgcg atggtcgcgc cgctagcgcc
     4441 gccgatcaga taaccgacgc ccatgaacag ggccgtgaga ccggccagaa gaattgctgt
     4501 ccgaaggtaa ttcatcatcc cctcccggta cgggcggcag ggcgccgcgg catacgccga
     4561 tcaggtaagg atcgcgcctg ctgcggttca agaccggcgg gtgcgtcgcc gcgtcgcgct
     4621 ttccccggag ttcgcgagga agcacctcgc gcatttgcgt agcgccaccc tcccagcaag
     4681 aaaaagggat aggctcaggc cttgcgggtg tgcccccagg cctcgtccca gtcctggccc
     4741 gcggcatcga cgacgcggcc gtcggcctcg aagaacttgt tggggacctg gaagatcgcg
     4801 agcatcagcc cgccttgcgg acaccaggcg acgtggcggc tgccctgttc gcgccagacg
     4861 aattcgccgg ccttgcactc gatgcccttg gccgggcctt ccttgtcgac aaggctgccc
     4921 tcaaggacat aggtctgctc gatgttgacg tgctcgtggt cgggcagcac cgcgcccggt
     4981 gcgaaccgca tcagcgcggt catcagaccc gtggtgcggt cgaacaagag cgtcttggcc
     5041 tcacagccgg ggaagcgcgt cttttgccac gccatgctgt cggggcgaac gatgtgagaa
     5101 tgcgtctcgg cggtcccaga gcccatcggt gtcacggcgt ccatggcagc ctccttgcgg
     5161 tcacggggaa tgataccagt tttgccaatg cccgccagcg ggcccccaaa tccgcggttg
     5221 acaccacgtg gcacgggagg cctcatggcc tccgaaaagg ctgcaaagac cctccccccg
     5281 agggaacctc ggcccctggc aaatacttca agaaacgcct cgaaaatccg aaggaaacac
     5341 cccatgcgca aaattctcgc cgctgccgtc ctctccgcca tatcggcctc gcttatgtca
     5401 gccgcgcccg cgcaggcgca gaccccgatc aagatcatgg tcggcgggat cgacaagcag
     5461 atctacctcc cggcaaagct tgctgcccag ctcggcttct tcagggaaca ggggcttgac
     5521 gtcgagctgt tcaactcgac ctccggctcg caagccgcga ccgcgctgtt ggcgcgcgag
     5581 gtgcagggcg tggtcgggtt ctacgatcac accatcgacc tgcaatccaa aggcaagttc
     5641 atcaccaatg tggtgcagtt cggcgtcgcg ccgggcgagg tggtgctggt gaaatccgcc
     5701 gacgccgaca agctgaaaga cccggcgaac tggaagggac aggcgctcgg cgtcaccggg
     5761 ctcggctcgg caacggattt cctcacccgc gcgcttgccg ccaaggcagg attgaaggtg
     5821 caggactaca cgctggtgcc ggtcggcgcc ggcgacacgt ttctggcggc gatgcagcag
     5881 ggcaagatcg tctccggcat gaccaccgag ccgacggtgg cgcgcgcgat caaggccggc
     5941 accgccaaag tcggcatcga cttgcgcacg ccggaagcaa cccgcgcggc gctcggcggc
     6001 gattatcccg ccgcctgcct ctacatggat cgcggctgga tggaagccaa caaggagacc
     6061 gcgcagaagg tcgtcaatgt gttcgtcaag accttgaagt ggatgaactc gcattcgccg
     6121 gaggaaatcg cggcgcagat gccgaaggat tattatgccg gcgagctcga cctctatgtg
     6181 cagggcctgc gcgaggggcg cgcgcaatat tcgcccgatg gcatgatgcc gcagggcgcg
     6241 ccggagtcgg tgctcaaggt gctgtcgagc ttctcgccca acgtgcaggg caagaccatc
     6301 gacctgtcga aaacctacac cacggagttc gtcatcaagg ccaacgcgac gcattgatac
     6361 gtcattccgg ggcagcccgc agggctgaac ccggaatctc gagattccgg gttcgccgct
     6421 ccgcgtcgcc ccggaatgac ggctgcaatt gggaaataca ctaatcatgg atgccagctt
     6481 acccaccgcc gtcgctctca aacatgtcgc ccggcgcttc gtcacgccgg cgggcgaaat
     6541 tctttcggcg ctggaggatt tcgatctctc gattgcgccg ggcgaatttt ttgccatcgt
     6601 cgggccgacc ggctgcggca agtcgacgac gctggggctg at
//
LOCUS       DATNOI010000475        18556 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1853092,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000475 DATNOI010000000
VERSION     DATNOI010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18556
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853092"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1203)
                     /locus_tag="VN968_18935"
     CDS             complement(247..1203)
                     /locus_tag="VN968_18935"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HXL31346.1"
                     /translation="MNPEPIFDLAHLGHMELLTPKPEESERFFVDVMGMTVSGRKGSS
                     VYLRGWDDYERYSLKLTASKTSGMEHMALRARSPQALERRVAALKGSGFEIGWTDGDM
                     GQGPAFRCRDPDGHIVELYYETEWYAAPPDQKPALKNQAQRFPARGVNVRRLDHLNCL
                     AVDIKANRIFFEKYLGCRLTEQIVLNDGTEAAMWMTMSNKSYDFAYSRDHSNTPGRFH
                     HVTYALDSREEILRAADIFLENGVYIETGPHKHAIQQTFFLYVYEPGGNRVEVANAGA
                     RLILAPDWKPIVWTEEERKKGQAWGLKTIESFHTHGTPPVGE"
     gene            complement(1219..2070)
                     /locus_tag="VN968_18940"
     CDS             complement(1219..2070)
                     /locus_tag="VN968_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31347.1"
                     /translation="MRLRTLLGDHPGTSALKNGSIKSDLVTLDFAEYSPTNKGFKPMV
                     RDGAFDVSEMAIVTYLMAKSFGNPMVLLPNVVLARFQHAYAVYRAELGNLRPSDLEGR
                     RVGIRSFTTTTGAWLRGILANDYGIDLNSINWVTFEDAHVAEFRDTTQRAPQGKQIIQ
                     MLLDGELDAVLGEKVERPGLKPLFADAATEERSWFAKHKVRPINHMAVVSANLSKDHP
                     EAVREVFRLLQASAALAPPAAAPHFSAEDMRRSLELIIQYTAEQGLIPRAFAVDELFD
                     DLTRSLR"
     gene            complement(2078..2770)
                     /locus_tag="VN968_18945"
     CDS             complement(2078..2770)
                     /locus_tag="VN968_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HXL31348.1"
                     /translation="MKTVVVRNIKRADAAVVKRLGALGASTVHEACGRDGLMKPYLRP
                     VWAGGEAAGTAVTVLVQPGDNWMIHVAVEQCRPGDILVVGCSADNTDGMFGDLLATSL
                     MARGVTGLVIDAGCRDVKSLREMGFPVWSKAISAKGTVKATLGAVNIPVVCAGINVLP
                     GDAVVADDDGVVVVRRQDAAEVAAKGEQRQADEEGKRKQLAAGALGLDMYNMREPLKK
                     AGLIYLDEPLSD"
     gene            complement(2952..4214)
                     /locus_tag="VN968_18950"
     CDS             complement(2952..4214)
                     /locus_tag="VN968_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL31349.1"
                     /translation="MSDFARPSALAPFRIRNYRFQWPADLLTSWAFEMETLILGWYVL
                     VETRSVLLLTVFASLSYVGTLIAPLFGVVGDRIGHRDLLAMMRATYALLAAMLMTLAL
                     TGHLSPLSVLVIVAVMGLVRPSDLGVRGALVATIMPHHQLIGAISISRTTMDTARIAG
                     ALSGAGLFAALGMGQAYVAIVSLYVVSTLLTLGVVAPWKPHPIDEAVDGVQRRSPMRD
                     LKEGVAYVWTTPRMRAAMWIAFLANLTAYPLSNGLLPYVAREIYGTSQTGLGYLSASF
                     AAGSLVGSIALSLVGGVRVARLLIGATIMWYGTLLVFAQMQTVPAAMACLLLAGFSQS
                     LCMISIAVILMRSSSEHFRGRVMGGVRMMVIYGLPLGLLAAGSLIDGIGFAATGTLYA
                     AAGMALMMAIVLHWRADLWHVHAPANAR"
     gene            complement(4349..6100)
                     /locus_tag="VN968_18955"
                     /pseudo
     CDS             complement(4349..6100)
                     /locus_tag="VN968_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(6508..8490)
                     /locus_tag="VN968_18960"
     CDS             complement(6508..8490)
                     /locus_tag="VN968_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HXL31350.1"
                     /translation="MIAELGHYALVLALALALIQSSSPVIGARKGDVALMKLADSTAL
                     AQFAFVVLAFGALTICYVTSDFSVANVYENSHSQMPLVYKFTSVWGNHEGSMLLWVLI
                     LAIFGALFALFGTNLPATLKANVLAVQSWIASAFYLFILFTSNPFRRLAEVPVEGRDL
                     NPILQDFGLAVHPPMLYLGYVGFSIAFSFAVAALIDGRIDAAWARWVRPWTLLAWMCL
                     TLGIAMGSYWAYYELGWGGWWFWDPVENASLMPWLAGTALLHSAVVMEKRNALKVWTI
                     LLAILTFSLSLIGTFLVRSGVLTSVHTFSSDPARGVFILLILVLFIGGSLALFAWRAP
                     LLKQGGLFAPISRESALLFNNLFLATACATVFVGTLYPLVLEALTGDKISVGAPFFNL
                     TFGPLFVPLLMAVPFGPVLAWKRGDLYGVAQRLLAAGGVAAVGLATTFAFAGTKAAFA
                     PFGIGLALFVMAGAITDIAERVDLRSMSVENIGRRAGGLPRSAWGTAVAHFALGMTLL
                     GIVCETNWSSERIVALKPTQTVSISGYDLTFDGMVSRDGANYRETAARFTVRKAGAVI
                     GVMEPSKRIFPSRDGMSTTEAALLTRGASQLYLSLGDNNADGSIAMWLYYKPMVLMIW
                     LGAMVMAIGGALSLSDRRLRIGAPKPAARILAQPAE"
     gene            complement(8528..9001)
                     /gene="ccmE"
                     /locus_tag="VN968_18965"
     CDS             complement(8528..9001)
                     /gene="ccmE"
                     /locus_tag="VN968_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HXL31351.1"
                     /translation="MTRKQRRLILIGSSLGVLAFAVALVLNSLRESIVFFNSPTDIAE
                     KKSAAGTRVRLGGMVKVGSLVRGDNLQLRFEVTDGNKDIPVSYHGIVPDLFREGQGVV
                     AEGRIEPGGTFMADTVLAKHDENYMPREVVETLKKQGHWQETAAPNPVPIKASPK"
     gene            complement(8998..10164)
                     /gene="ccmI"
                     /locus_tag="VN968_18970"
     CDS             complement(8998..10164)
                     /gene="ccmI"
                     /locus_tag="VN968_18970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HXL31352.1"
                     /translation="MILWPILTLMTLGAVIAVWWPFARRPGSVRSGSDIAVYRDQLDE
                     IDRDQAASLIGNVEAEAARVEVSRRLIAAAGTAKARSVAPAPGPAGWYRRATLAAAIV
                     LLPVGAGAVYLSLGSPGLVSVSMNASTDGRPLPAGIEHTVAEVETYMESNPRNGRGWE
                     LLAPVYLRLGRFDDAVRARRNTLEIFGPDAAHLGDLGEAMVMASGGAVTAEAKSLFER
                     ANAADPDDVMAQYYLGLGAKQDGRRDEAEKRWRALVSRAGEGAEWLPVVKDALARIDE
                     KSPSVVVAAPASGAAKVAPKGPSAREVAAAAQLAPAERNGMIEGMVARLAQKMAENGS
                     DVEGWLRLIKAYSVLGERDKALTAAANARSALAGNNDNLRRIGELAKELGLEGS"
     gene            10606..12357
                     /locus_tag="VN968_18975"
     CDS             10606..12357
                     /locus_tag="VN968_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL31353.1"
                     /translation="MPDLITVTSFGRVISVRGSLARVGLLAVGQIPASEIRATVGRFV
                     SIRCANSTIVAMITEVSCEDLPTSDTYIASASVDLLGEILGGPDRPKFQRGVTNYPTI
                     GDAVELITNQELRTVYAPTGSDQINVGTLQQDPSVIAYVDVEEMLSKHFAVLGSTGVG
                     KSSGVSLLLNEILKARPSLRVFLLDVHNEYGRSFGDRALVLNPRNLKLPFWLFNFEEI
                     VDVLFAGRPGVPEELDILAEVIPTAKGIYTQYQNSDRMGLKRADPKAVGYTVDTPVPY
                     RLVDLISLIDERMGKLENRSSRILYHKLISRIETVRNDPRYAFMFDNANVGGDTMAEV
                     ISYLFRLPANGRPMTIMQLAGFPAEVVDSVVSVLCRMAFDFGLWSDGVSPMLFVCEEA
                     HRYASADRSIGFGPTRKAVSRIAKEGRKYGVYLGLVTQRPAELDATIISQCNTLFAMR
                     LANERDQALLRSAVSDAAANLLSFVPSLGTREVLAFGEGVALPTRLRFKEVPSHQLPR
                     SEATIATVSSVAAGHDMHFVGAVLERWRGATSQRDVPNDPGLNDRPAARTVEAPMLQP
                     SMGLDPDRFSLLKKPLR"
     gene            13108..13800
                     /locus_tag="VN968_18980"
     CDS             13108..13800
                     /locus_tag="VN968_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HXL31354.1"
                     /translation="MAWRKTAAVVTLAMVPTFAAISGAMADQRQHHYGDASDEQPLGV
                     AVSASSSDAAWIASQLHQPRFAARRDQPVMSLPRDSRADANAMPDGPHVNGLRSDGEP
                     TRVVAVAERYRGTNPTGHSRAWCALFANMVLARTGYGGTGSAAARSFARYGRAASGPA
                     PGVIAVWPHHVGFVVGAVAPGRIRVISGNHNHRVAEGTYSTRSVMAFRYPDRGSRSMA
                     GFASASNRHAPL"
     gene            complement(14053..14475)
                     /locus_tag="VN968_18985"
     CDS             complement(14053..14475)
                     /locus_tag="VN968_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31355.1"
                     /translation="MQIRTSRRSSRRAYDDEWLKWTAPAAVVMSIASPAHAAQYMSVN
                     EAQKHAFPNATGFAEVQAGRVWKAQSEAVRPWISYVHIAFGLAVPAVIVAHITVGRAS
                     TATKKAQMPRATAAAPVPTAATLVPADEPAEAILGNVS"
     gene            complement(15178..16566)
                     /locus_tag="VN968_18990"
     CDS             complement(15178..16566)
                     /locus_tag="VN968_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31356.1"
                     /translation="MQTLESELKQKQEQTPRTAQSLSAGRPTVAGKPTGPYAQAPAAD
                     APAASPNKNNNDLFGVAQAPVPGLKIGMYGEIKYGSQQNPAHNGQWQNGFDMGRLVLL
                     PTYQVTDNIIFNAEIEFEHAGSAFDNDDKLHGAAEIEQAFFDFRVNDHLTLRSPGVDL
                     VPISFNNLYHEPTLFYSVERPELANGLIPTTWVAGSAGFYGKIVDGLNYQFQVSHSAE
                     DFGDGFDHRGDNGAPIAGGYAAGPNGADALGFAKAPIGGFAQMSNQAAVTGRLSYQPG
                     FLPGFAGSTAVYYSPDITPRGAYGDLPDGTTRPLGKNSLTIFDTEARYRVPNTGLELR
                     AEYAHVGFGNPENLRANNDGDDANNVGKNMWGYSGEIAYHFKMGTILGSEWEAVPFYR
                     YTRQNLQTAGLFGVDPNGMTGSGDMTFHDIGVAVFPNPSLVLKLNYTKALDRSATGPM
                     ADKVLGGVGWLW"
     gene            16565..16714
                     /locus_tag="VN968_18995"
     CDS             16565..16714
                     /locus_tag="VN968_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31357.1"
                     /translation="MRFSMAWSFFNSISFVALDALSSPASTAALLPPSINEAISTVDV
                     ERPMP"
     gene            complement(17642..18061)
                     /locus_tag="VN968_19000"
     CDS             complement(17642..18061)
                     /locus_tag="VN968_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HXL31358.1"
                     /translation="MNLKNGDTVTSPFKVVFGLSPNMGVAPSGVEKANVGHHHLLIDK
                     ALTPEEMTQPIMVDEQHVHFGKGQTETTVTLPPGKHTLQLMLGDWTHIPFKPPVQSEV
                     ITVTVKGAAATPEPHAMMVKEKHHHHHHHHHHHHHHS"
BASE COUNT         3561 a         5916 c         5602 g         3477 t
ORIGIN      
        1 gtgccagcgg cgtcgacggc aggtccctcg atgccgcctg tatattcacc gggcggagta
       61 agctgacggc tctcgaacag cgcgggctcc gcgcctgcgg caccacgaag agctaccagg
      121 cccgccagga cgctcgcgcc taccagcttt gccaccatct tgttctgcct tggccgggct
      181 tgttgtcgag gtccgcggcg gccttgattt ttgtaacggc ggaagccgag ttcgctatcc
      241 cccacctcac tccccaaccg gcggcgtgcc gtgggtatga aacgactcga tcgttttcag
      301 cccccacgcc tggcccttct tgcgctcttc ctcggtccag acgatcggct tccagtcggg
      361 ggcgaggatg aggcgggcgc cggcgttggc gacttcgacg cggttgccgc cgggttcata
      421 gacgtagagg aagaacgtct gctggatcgc gtgcttgtgc gggccggttt cgatgtagac
      481 gccgttttcc aggaaaatgt cggcggcgcg caaaatctcc tcgcggctgt cgagcgcgta
      541 ggtaacgtga tggaaacgtc ccggcgtatt ggagtgatcg cgcgaatagg caaagtcgta
      601 actcttgttc gacatcgtca tccacatcgc ggcttcggtg ccgtcgttga gcacgatctg
      661 ttcggtgagc cggcagccga ggtatttttc aaagaagatg cggttggcct tgatgtcgac
      721 ggcgaggcaa ttgaggtgat ctaggcggcg gacattgacg ccgcgcgcgg gaaagcgctg
      781 cgcctgattt ttcagcgcgg gtttttggtc gggcggcgcc gcgtaccatt cggtctcgta
      841 atagagctcg acgatgtggc cgtcgggatc gcggcagcgg aacgccgggc cctgccccat
      901 gtcgccatcg gtccagccga tttcgaaccc cgatcccttc agcgcggcga cgcggcgttc
      961 cagtgcctgc gggctgcgtg cccgcagcgc catgtgctcc atgcccgagg ttttcgaggc
     1021 ggtcagcttg agcgaatagc gctcgtaatc gtcccagccg cgcaggtaga ccgacgagcc
     1081 cttgcggccg ctgacggtca tccccatcac atcgacgaaa aacctttcac tttcctcggg
     1141 tttcggcgtc agcaattcca tgtggccaag atgggcaaga tcgaagatcg gctcggggtt
     1201 cattcgctat ctctccattc agcgcagcga ccgcgtcagg tcgtcgaaca attcgtcgac
     1261 cgcgaaggca cggggaatga gcccctgctc cgcggtatat tggataatca attcgagcga
     1321 gcggcgcatg tcttcggcgc tgaagtgcgg cgcggcggcc ggcggcgcca gcgccgcgct
     1381 cgcctgcagc agccggaaaa cttcccgcac cgcctcgggg tgatccttgg acaggttcgc
     1441 gctgaccact gccatatggt tgatcggccg taccttgtgt tttgcaaacc atgatcgctc
     1501 ttctgtggcg gcatcggcga acagcggctt caggccggga cgctccacct tctccccgag
     1561 caccgcatcg agttcgccgt cgagcagcat ctggatgatc tgcttgccct gtggcgcacg
     1621 ctgggtggta tcgcgaaatt cggcgacatg ggcgtcctcg aacgtgaccc agtttatcga
     1681 attgaggtcg atgccatagt cattggcgag aatgccgcgc agccacgcac cggtcgtggt
     1741 ggtgaaggaa cggatgccga cgcgccggcc ctcgagatcg ctcggcctta gatttcccag
     1801 ttcggcccgg tagacggcgt aggcgtgctg gaaccgcgcc agcaccacgt tcggcagcag
     1861 caccatcgga ttgccaaagc ttttggccat cagataggtg acgatggcca tctccgagac
     1921 gtcaaaagcc ccgtcgcgca ccatcggctt gaaacccttg ttggtcggcg aatactcggc
     1981 gaaatccagc gtgaccagat ccgacttgat cgagccgttc ttcagcgccg atgtgccggg
     2041 atggtcgcca agcaaggtgc gtaagcgcat cgtcatttca atcggacagg ggttcatcga
     2101 gatagatgag gcccgccttc ttcagcggct cgcgcatatt gtacatgtcg agcccgagcg
     2161 cgccagcggc aagctgcttg cgcttgccct cttcgtcggc ctggcgttgt tcgcccttgg
     2221 ccgcgacctc ggcggcatcc tgccggcgca cgaccacgac gccatcgtca tcggcgacca
     2281 cggcgtcgcc cggcaacaca ttgattccgg cgcagaccac gggaatgttg accgcgccca
     2341 gcgtcgcctt gaccgtgccc ttggccgaaa tcgccttcga ccacaccgga aagcccatct
     2401 cgcgcagcga tttcacgtcg cggcagccgg catcgatgac gaggccggtg acgccgcgcg
     2461 ccatcagcga ggtcgccagc agatcgccga acatgccgtc ggtattgtcg gcgctgcatc
     2521 cgaccaccag aatgtcgccc ggccggcact gttcgaccgc cacatggatc atccagttgt
     2581 cgccgggctg taccagcaca gtgacggcgg tgccggccgc ctcgccgccc gcccacaccg
     2641 ggcgcagata gggcttcatc agcccatcac ggccgcatgc ttcgtgcacg gtcgaagcgc
     2701 cgagcgcgcc gagccgtttg acgaccgccg cgtcggcccg tttgatgttg cggacaacga
     2761 ccgtcttcat gccagttcct ccaaacttat ggacccagag cgggatgatt tttcttcgaa
     2821 tcgtcatccc gctctatctt tttgtttgag catgatcttt tcggaaaacc ggtacccact
     2881 tttccggatc atgctagagc cagagacatc ctatcagatt caagccgcag gggccgaggc
     2941 tgaggccgcg ctcacctggc attcgccggc gcatggacat gccaaagatc ggcgcgccag
     3001 tgcagcacga tggccatcat caacgccatc ccggccgccg cgtaaagggt gccggtcgcg
     3061 gcaaatccga tcccgtcgat caggctgccg gccgccagca ggccgagcgg cagaccatag
     3121 atcaccatca tgcgcacgcc gcccatcacc cggccgcgaa aatgttcgct cgagctccgc
     3181 atcaggatca ccgcgatcga aatcatgcaa aggctttgcg agaaaccggc cagcagcagg
     3241 cacgccatcg cggccggcac ggtttgcatt tgcgcaaaca ccaacagcgt gccgtaccac
     3301 atgatcgtcg caccgatcaa aagccgcgct accctgacgc cgccgaccag gctcaaggcg
     3361 atcgaaccga ccagcgagcc tgccgcgaaa ctggccgaca gatagccgag cccggtctgg
     3421 ctggtgccgt aaatctcgcg cgcgacatag ggcagcaatc cgttggacag gggataggcg
     3481 gtcaggttgg cgaggaacgc gatccacatc gccgcgcgca tccgcggcgt ggtccagaca
     3541 taggccacgc cctccttcag gtcgcgcatc ggcgagcggc gctggacgcc atccaccgct
     3601 tcatcgatcg ggtgcggttt ccagggcgcc acgacgccga gggtcagcag cgtgctgacg
     3661 acataaaggc tcacgatcgc cacataagcc tgtcccatgc cgagtgcggc gaacagcccg
     3721 gcgccgctca gcgcgcccgc gatgcgcgcg gtgtccatcg tggttctgga aatgctgatc
     3781 gcgccgatca actggtgatg cggcatgatg gtcgcgacca acgccccgcg caccccgaga
     3841 tccgacggcc gaaccaggcc catgacggcc acgatcacga ggactgagag cggactcaaa
     3901 tgcccggtca gcgccagggt catcagcatg gccgccagca gcgcataggt cgcgcgcatc
     3961 atggcgagca ggtcgcggtg accgatgcga tcgccgacca cgccaaacaa cggggcaatc
     4021 agcgtgccga cgtagctgag cgaggcgaac actgtcagca gcagcaccga tcgggtctcg
     4081 accagcacgt accagccgag aatgagcgtc tccatctcga aagcccacga ggtgagcagg
     4141 tcggcgggcc actggaaacg gtagttcctg atacggaagg gggcgagcgc ggaaggccgc
     4201 gcgaagtcac tcaaaatccg aagccgcgtt tcatcgcgtt ccacccctgc cctgtttctt
     4261 gttgggacca gatctacagc ctggcggccg ccggggccag ccctgccgcg tcgcaaaaca
     4321 cggtcaatcg cccgccgccg ccggcggttc aggccgcgga cacggcctca tcgcggcgcg
     4381 cgtgcaacaa ccgctgtatc tcggcgcagg ggatcgccgg gctgaacaga tacccctgca
     4441 tctcggtaca acccagttcg cgcagcaatt ccatttgcgg caccgtctcc acgccctcgg
     4501 ccgtcgtcgt catgttgctc gcggcagcta tgttcacgat ggcctggaca atcgaggatg
     4561 atccagccgg gccggcaatg tctttgacaa agcagcgatc gatcttgatc ttgtcgaagg
     4621 gaaaacgctg cagatagctc agtgaggaat agccggtgcc aaagtcgtcc agcgctatcc
     4681 ttacccccaa ctctcgcagc tggtgcagca gggcgagcgc cacttcatcg tcacggatca
     4741 ggatcgcctc ggtaatctca agttccaacc tgcacggcgc gaggccggag gttgcgagtg
     4801 ccgtggccac attcagcgcc agggtctcac tcttgaactg aatcggcgag acgttgaccg
     4861 cgacccggat gtgatccggc cagctcgcgg cctcgatgca cgcggtgttg agtacccagt
     4921 caccgagctg gttgatgaga ccggtctctt cggcgatcgg aatgaattcc gcgggcgata
     4981 tcatgccccg cttggggtgg cgccaacgca gcagcgcttc gcagctgctg atcttgttat
     5041 ccctgagatt caccaacggc tgatagtaga cctcgaaccc gccatccatg atcgcctgac
     5101 gcagctcaag ttccaatatt cgccgcgtct tgacgcgggc atccatgctc agttcaaaga
     5161 atcgataggt gcggcgtccg tcggccttgg cctcgtacat ggccatgtcc gcgttcttca
     5221 gcagttgatc cagatccgcc ccctgctgct gagccagagc aatgccgatg ctggcgtccg
     5281 tcgtgatatg atggccaccg caatcgtagg gtttccggat cgcttcataa attctttcga
     5341 ccagatgcgt cgtatccgac ggatgtttta tcgcggtctg aatgatggca aattcgtcac
     5401 cgccgagccg ggccgcgaca tcggcccctc cgagacagcc gcgcagccga ccagccaccg
     5461 ctttcaggag ttcatcaccg accgaatggc ccagagcgtc attgacgctc ttgaactcgt
     5521 cgatatcgat gtacagaacc gccaactgtt ggtcgggccg cacgttctcg agggcgcgtt
     5581 cgagttgctc tcgaaacaac acccggttag gcagatcggt gagcgcgtcg taatgcgcca
     5641 aatggacgat tttctcgtcc gagcgtttgc gctccgtgac gtcctcgatg gtggccaccc
     5701 acccgcccgc ttccagcggc tggttgataa tctggatcgc cctgccgtct gcggtatcca
     5761 cgatctgatg agtaatcttg ccgagcgcca cgttgcgtct gatagagtcg caaaattcgc
     5821 cgacatcccc gctaaaggat cctgtctctt tccgatgcga aatcagctcg tacatggtgc
     5881 agcccggctt ggccacatcc ggagacagcc caaacatttc aatgtagcgc tggttgcaaa
     5941 cggtaacccg tgacgcattg tcgtaaagaa caaggccttg cgggatattg ttcacggcga
     6001 cgctgagctc gcgccgttcc acagccagcc ttcggttgga cgattggtgc ttcagccaga
     6061 gacgagcgat cgaagcagca atgaaggcga tcacgagcgc gcaaagaacg atcgcaccga
     6121 tcagcaattt ggttggttgc cccccatgca cccacgccaa cgacaccgtt gcgatgatcg
     6181 agaccgaaaa aatgccgcaa aaagtcagcc gccgatcgac atcgccgccc aggaatccat
     6241 agtcgctgtc aggatctact tcgctcatgc ctcccccgat cgtgacagcg ttaccgatca
     6301 cagatggaaa cgacgttacg agttgcggtt agcaagccat taatggcggg ctaacagtct
     6361 cgtcgcttcg ggttctcggc gggaggagaa ggcgcctgtg gtaatcgatc accgcagcca
     6421 ggcgctcgtt ttgggacagc ttgcggaatc aggccagagc ggcgccgcag ccggccgcgc
     6481 cgcaaccccc gcggctggaa gagaaaacta ttcagcaggc tgcgccaaaa tcctcgccgc
     6541 cggcttcggc gcgccgatcc gcaatctgcg gtccgacaac gacagcgcgc cgccgatcgc
     6601 catcaccatc gcgccgagcc agatcatcag caccatcggc ttgtaataga gccacatcgc
     6661 gatcgacccg tcggcattgt tgtcgccgag cgacagataa agctggctgg caccgcgcgt
     6721 cagcagcgcc gcctcggtgg tcgacatgcc atcgcgtgac ggaaagatgc gtttcgacgg
     6781 ctccatgacg ccgatcaccg cgccggcctt gcgcacggta aagcgcgcgg ctgtctcgcg
     6841 atagttcgcc ccatcccggc tcaccatgcc gtcgaaagtc agatcgtagc cgctgatgga
     6901 aaccgtctgc gtcggcttga gcgcgacgat acgctcgctg ctccagttgg tctcgcagac
     6961 aattccgagc agcgtcatgc cgagcgcaaa atgcgccacc gccgttcccc aggccgaccg
     7021 cggcaatccg cccgcgcgac gcccgatgtt ctcaacagac atgctccgca gatcgacgcg
     7081 ttcggcaatg tcggtgatgg cgccagccat cacaaaaagg gcaagcccta ttccaaacgg
     7141 cgcgaacgcg gccttggtgc cggcgaaggc aaaagtcgtc gcgaggccca ccgccgcaac
     7201 acctcccgcc gcgagcaaac gctgcgcaac cccgtaaaga tcgccgcgct tccatgccag
     7261 caccggtccg aacggcaccg ccatcagcag cggcacgaac agcggcccga atgtaagatt
     7321 gaagaacggc gcgccgaccg aaatcttgtc cccggtgagc gcctccagca ccagcggata
     7381 aagtgtgccg acgaacaccg tggcgcaagc cgttgcgaga aacaggttgt tgaacaacaa
     7441 cgcgctctcg cgggagatcg gcgcaaacag gccgccctgc ttgagcagcg gcgcgcgcca
     7501 ggcaaacagc gcgaggctgc cgcctatgaa cagcacaagg atcaacagga tgaaaacgcc
     7561 gcgcgcggga tcgctcgaga aggtgtggac cgacgtcagc actcccgagc gcaccaggaa
     7621 ggtgccgatc agcgacagcg agaaggtgag aatggccagc agaatggtcc acaccttcag
     7681 cgcatttcgc ttttccatca ccaccgccga atgcagcaat gcagtgccgg cgagccacgg
     7741 catcagcgac gcgttctcca ccggatccca gaaccaccag ccgccccagc cgagctcgta
     7801 ataggcccaa taggacccca tcgcgatgcc cagcgtcagg cacatccagg cgagcagcgt
     7861 ccacggccgg acccagcgtg cccacgccgc atcgatgcgg ccatcgatca gcgccgccac
     7921 cgcaaacgag aatgcaatgg agaaaccgac atagccgagg tagagcatcg gaggatgcac
     7981 ggcgaggccg aaatcctgca ggatcggatt gagatcgcgg ccctcgaccg gcacctcggc
     8041 cagccgccga aacggattcg aagtgaagag gatgaaaagg tagaaggcgg acgcgatcca
     8101 ggactgcacc gcgagcacgt tggccttcag ggtcgccggc aggttggtgc cgaacaacgc
     8161 gaacaacgcg ccgaatatcg cgaggatcag cacccacaac agcatggagc cctcgtggtt
     8221 gccccacacg ctggtgaatt tgtagaccag cggcatctgc gaatgggaat tttcgtagac
     8281 attggcgacc gagaaatccg acgtgacgta gcagatggtg agcgcgccaa aggccagcac
     8341 aacgaaggca aattgcgcca gcgcggtgga gtcggcgagt ttcatcagcg caacatcgcc
     8401 cttgcgcgca ccgatcaccg gcgacgacga ttggatcagc gccaatgcaa gcgccagtac
     8461 cagcgcgtag tggccaagtt cggcgatcat gtcgatggct ccgagttctc gtccggcgtg
     8521 tcgccgctca ctttggactt gccttgatcg ggacagggtt tggcgccgcg gtctcctgcc
     8581 agtgtccctg cttcttcaag gtttcgacca cctcgcgcgg catgtaattc tcgtcatgct
     8641 tggcgagcac ggtgtccgcc ataaaggtgc ctcccggctc gatgcgtccc tcggcgacca
     8701 cgccctgccc ttcgcgaaac aggtcgggca cgatgccgtg gtagctgact ggaatgtcct
     8761 tgttgccatc ggtcacctca aagcgaagct gcagattgtc gccgcgcaca aggctgccga
     8821 ccttcaccat gccgccgagg cgaacgcggg tgcctgccgc ggactttttc tcggcgatgt
     8881 cggtcggcga attgaaaaac acgatcgact cgcgcagcga gttcagcacc agcgccactg
     8941 cgaaggccag cacgccgaga cttgagccaa tcaagatgag gcgccgctgc ttccgtgtca
     9001 tgagccttcc agtcccagct ctttcgccaa ttcaccgatg cggcgcagat tgtcattgtt
     9061 gccggcgagc gcgctgcggg cattcgccgc agcagtgagc gccttgtcac gctcgccgag
     9121 caccgaatag gccttgatca ggcgaagcca tccctcgacg tcggagccgt tctcagccat
     9181 cttctgggcc aggcgagcga ccatgccttc gatcattccg ttgcgctcgg ccggcgccaa
     9241 ttgggctgcg gcggcgacct cgcgcgcgct cggccccttc ggtgcgacct ttgccgcgcc
     9301 cgatgccggg gccgcgacaa cgaccgatgg gctcttctcg tcgatccgcg cgagcgcatc
     9361 cttgaccacc ggcagccact ctgcgccctc cccggcccta gacacgagcg cgcgccatcg
     9421 cttctccgcc tcgtcgcgac ggccgtcctg cttggcgccg agcccgagat aatattgcgc
     9481 catcacgtcg tcgggatcgg cagcattggc gcgctcgaac aggctctttg cctccgcggt
     9541 gactgcgccg cccgaagcca tcaccatcgc ttcgccaaga tcccccaaat gcgcggcgtc
     9601 cggaccgaat atctccagtg tattccgtcg cgcccttacg gcatcgtcga agcgtccaag
     9661 gcgaagatag accggcgcca gcaattccca gccccgtccg ttcctgggat tgctctccat
     9721 ataggtctcg acctcggcga cggtatgctc gattccggcc ggaagaggtc gcccgtcggt
     9781 tgacgcgttc atcgatacgg aaacgagacc aggcgatccg agcgagagat aaacggcacc
     9841 ggcgccgacc gggagcagca cgatggcggc ggccagcgtt gcccggcggt accagccggc
     9901 cgggccaggc gccggcgcga cggatctggc ttttgcggtc cctgctgctg cgatcaagcg
     9961 gcgtgaaact tcgaccctcg ccgcttcagc ctcgacgttc ccgatcaggc tcgcagcctg
    10021 atcgcggtca atctcgtcga gctggtcgcg atagacggca atgtcgcttc cagagcggac
    10081 agaccccggc cgccgcgcga acggccacca cacagcgatg accgccccga gcgtcatcaa
    10141 cgtcaaaatg ggccacagaa tcatctagtc actttaccga gcgctgcctg agcagtcttg
    10201 tatgcaccga cgtgctgccg cgattccaag cgggcagaag cgaactttga tgtatatcag
    10261 acgatggtcg aacagcgcgc cgaatacagc aacctcctgt catttgtttc gatattatcg
    10321 cgaccacgcg atcgacccac cgaccatctg aacgtttgca tcgaaccgcc gtcctcagcc
    10381 attggtaaac caggcgttaa acccgacgcg ccggcggcgc cgacggacgg aggtcaaaaa
    10441 caccgagttt ccagcagaat ccaccgataa agacccgccg gggcggcccg gcaaaatcga
    10501 aatactgacc aagatgtcgc ggatttgacg aacccggggt tcgccgcgct taaccgtttc
    10561 tccaacccga acctgcatag tcatgcacag atgtgaattc atgacatgcc ggatttgatc
    10621 actgtgacat ccttcggacg cgtaatttcg gtgcgtggat ccctcgcccg ggttggactg
    10681 ctggcggtgg gccagatacc cgcctcggaa ataagggcga ccgtcggacg gttcgtcagc
    10741 atccgctgtg caaattcgac catcgtcgcc atgatcacgg aggtatcctg cgaagatctg
    10801 ccgacgtccg acacctatat tgccagcgca tcggtcgatc tgctcggcga aatcctcggc
    10861 ggcccggacc gccccaaatt ccagcgcggt gtcaccaact atcccaccat cggcgatgcc
    10921 gtcgagctga tcaccaatca ggaactgcgc acggtctatg ccccgaccgg ctcggatcag
    10981 atcaatgtcg gcaccttgca gcaggatccc tccgtcatcg cctatgtcga cgtcgaggag
    11041 atgctctcga aacacttcgc ggtgctcggc tctaccggcg tcggcaaatc gagcggcgta
    11101 tcgctgctgc tgaatgaaat tctcaaggca aggccgagct tgcgggtttt cctgctcgac
    11161 gttcacaacg aatatggccg cagtttcggc gaccgcgcgc tggtactcaa tccgcgcaat
    11221 ctgaagctgc cgttctggtt gttcaatttc gaagagatcg tcgacgtgct gttcgcgggc
    11281 cggcccggcg tgcccgagga actcgacatt ctcgccgaag tcattccgac ggcgaaggga
    11341 atctataccc agtaccagaa cagcgatcgg atgggattga agcgcgccga tcccaaagcg
    11401 gtcggctaca ccgtcgatac gccggtaccc taccgcctgg tcgacctgat ttcgctgatc
    11461 gatgagcgca tgggcaagct cgagaaccgc tcatcgcgca tcctctatca caagctgatt
    11521 tcacgcatcg aaaccgtgcg caacgatccg cgctatgcgt tcatgttcga taacgccaat
    11581 gtcggcggcg acaccatggc ggaagtgatc agttatctgt tccggctgcc ggccaatggc
    11641 cggccgatga ccatcatgca gcttgccggc ttccccgccg aagtcgtcga ctccgtggtc
    11701 tccgtgctgt gccgcatggc gttcgatttc ggcttgtgga gcgacggcgt ttcgccgatg
    11761 ctgttcgtct gcgaagaggc ccatcgctac gcctcggccg atcgcagcat cggttttgga
    11821 ccgacgcgca aggcggtctc gcgcatcgcc aaggaaggcc gcaaatacgg cgtatatctc
    11881 ggcctcgtca cccagcgtcc cgccgaactc gatgccacca tcatttccca gtgcaatacc
    11941 ctgtttgcga tgcggctggc caacgaacgc gaccaggcac tgctgcgttc ggcggtctcc
    12001 gatgccgcgg caaacctgct gtcgttcgtt ccctcgctcg gtacccgcga agtcctggcc
    12061 ttcggcgaag gcgtggcgct tccgacccgg ctgcgcttca aggaagtgcc gtcgcatcaa
    12121 ttgccgcgca gcgaagccac gattgcaacg gtttcgtcgg tcgccgcggg ccacgacatg
    12181 cacttcgtcg gcgccgtgct cgagcgctgg cgcggagcta cttcgcaacg cgacgttccc
    12241 aacgatcccg gcctcaacga ccggccagcc gcacgcacgg tggaggcacc gatgctgcag
    12301 ccatcgatgg gtctcgatcc cgaccgcttt tcgctgctga aaaagccgct gcgctgaagg
    12361 tttggcgccg cggttttccc gcgcacttct cgccgctgcg cgcgggcgac gccatcgcct
    12421 tctctcgccg gtcatctcac caggccgtcc gatcccgacg ggctgcggcc ctcgcacccg
    12481 cgcgcgcggc tcgctattgc cgaagcgtta attgaatcag acgcattccc accgtcgtgc
    12541 ccgtgagttg gatgcaccac cgcaattccc accgtaatat tgtcagcatc attccaactt
    12601 ggggcgatag ctcgactacg gtgccacctc acctgaagtg atggcttgat gcctgtcttg
    12661 attgtgtggg tttcgatttt cgccgcggca gcgccgggat aacgccgcat ccatggcgac
    12721 gccgatccgg tcgatggcgc tttcgacgat cgcatcctta tcgcgtcggt gagaatgagc
    12781 acgtccgatt cgttttccaa tcgaagacaa catggggtca taattcggcc tacagcgatt
    12841 gcgaacttct gtgcagggag aaaacaaatg aaacgcctcc ttctgctcct tgccatggtg
    12901 atgttgctcg ctccgacgca cacgcaggcc aagggaagct cgtatccgag aacagcgccg
    12961 atcccgatcg cagtctcgtt ggtgagtccc gctccgctct cagcaaggga cgtccagcga
    13021 catgccaccg gcgattgcag cggcgcatgc caaagtcgga tcttccgacg agccaaggcc
    13081 tcatttcagt aacgcgtggg agtattcatg gcgtggcgca agactgcggc cgtcgtgacg
    13141 ttggccatgg tgccgacgtt tgcggcgatc agcggcgcga tggcggatca acgacagcat
    13201 cattatggtg acgcctccga cgagcagccg ctcggcgtcg cggtgtccgc cagcagcagc
    13261 gacgctgcgt ggatcgcctc tcagctgcat cagccgcgct ttgcagcccg tcgcgaccag
    13321 ccggtgatgt cgctgccgcg cgacagccgc gccgatgcga acgcgatgcc cgatgggcca
    13381 catgtgaatg gattgcgcag cgatggcgag ccgacacggg tagtggcggt cgcggagcgt
    13441 taccgcggca ccaatccgac cgggcattcc cgcgcctggt gcgccctgtt cgccaatatg
    13501 gttctcgcgc gcaccggata tggcggcacc ggctcagccg cggcgcgctc gttcgcccga
    13561 tatggccgcg ctgcgtccgg accggcgcca ggcgtcatcg cggtgtggcc gcatcacgtc
    13621 gggtttgttg tcggcgcggt ggcgcctggg cggattcgcg ttatcagcgg caaccacaat
    13681 catcgggtcg ccgaaggcac ctactcgaca cgcagcgtga tggcgttccg ttatcccgat
    13741 cgcggcagcc gcagcatggc gggattcgca agcgcatcga accgtcacgc accgctttga
    13801 tgcgagccag aagagggatg ccgacgctgg caaccggctc ggccgtgctg tcaatttcaa
    13861 tgcgacgatc gggtgcctgc ggcggccttg aattccgccg gttcaaaccg gtggatcctt
    13921 cacaaccgag acctcgagcg tcgtgccatc gtcagcgctc gccaacggcg cgaacgccgt
    13981 cacactcgct accgctgttg ccgtcttgat gatgaatttc gttgcgggtt ttcttaatcc
    14041 tcgggggtga actcaactga cgttgcccag gattgcctcg gccggttcgt cggcaggcac
    14101 cagtgtagcg gccgtaggca ccggcgcagc ggcggtagcg cggggcatct gcgccttctt
    14161 ggttgccgtg ctggcacggc cgaccgtgat gtgggcgacg atgaccgccg gaacggcgag
    14221 cccgaaggcg atgtgcacat agctgatcca gggccgaacg gcctcggact gcgccttcca
    14281 gacgcggccg gcctgaacct cggcgaagcc ggtagcattc ggaaaggcgt gcttctgcgc
    14341 ttcgttgaca ctcatatatt gagcggcatg tgccggcgaa gcgatcgaca tgacgaccgc
    14401 ggcaggtgcc gtccacttca accattcgtc atcatacgcc cttctacttg atcttcttga
    14461 tgttcgaatc tgcatacccg gcgccgctgg tatcacggag gcgccgcaaa gcattttgag
    14521 atgtgagact tacaacccgc gatgcaaaga cgacggcatg cgtgccgcac tacttgctct
    14581 gcgagtgata gttggattcg attgcggcga gatcctgtgt cgaaatcagg cattttgccg
    14641 gtgcggctga ggaataacca tgttgcgatt tttgacgagt cgccaacgcg ccgcccgagc
    14701 gctccggcgt cgcgacgatg tttgccgcga cgccgcggcc gcgctgctcg cgccggcgtc
    14761 tcgcgcaggc atcgtcaatg cccggcggcg cgaagccaat atctgaatct gcccgaccgc
    14821 gaagaaatgt tggaatttca gcgtggaagc ggcgcgcgcc actgtcttga gcagcgcaat
    14881 tcgcgcttcc cgtgtgctcg cgtcggtgcg cgcttcatca accgccgttg cggttcatga
    14941 cggcgttgag cgcctgtagg gactgctctt gagaattcaa ccgtcgccgt cgcgagcgcg
    15001 gcgatgatgt cggaatggcg acgccgggcc ggattggatg atcgtccgaa cggcaagttc
    15061 gagcgatggg cgcgcgccga cctgacggtc gctgggaccg ctcttaaagg caatgccgcg
    15121 gcaggcagcc gcggcatttt ggcttatcga gccgttaccg atcgttttat cgcgccacta
    15181 ccacagccag ccgacgccac cgagcacctt gtcagccatt ggcccggttg cagaacgatc
    15241 gagtgccttg gtgtagttca gtttcagcac caggctggga tttggaaaca ccgcgacgcc
    15301 aatgtcatgg aaggtcatgt cgccgcttcc ggtcataccg ttcggatcga ccccgaacag
    15361 gcctgcggtc tgcaagttct gacgggtgta gcgatagaac ggcacagcct cccattcgct
    15421 gccgagaatc gtccccatct tgaagtgata ggcgatctcg ccggagtagc cccacatgtt
    15481 cttgccgaca ttattggcgt catcgccatc gttgttggcg cgcagatttt ccgggttgcc
    15541 gaagccgaca tgggcatatt cggcccgcag ctccagtccg gtattgggaa cgcgataacg
    15601 cgcctcggta tcaaagatcg tcaggctgtt cttgccaagc ggcctggtcg tgccgtccgg
    15661 caggtcccca taggcgccac gcggcgtgat gtcaggggaa tagtagaccg ccgtgctgcc
    15721 ggcgaagccg ggaaggaatc cgggctggta gctcagacgc cccgtcacag cggcttggtt
    15781 gctcatctgt gcgaagccgc cgattggcgc ctttgcaaat ccgagggcgt cggcgccgtt
    15841 tggcccggcc gcgtagccgc cggctatcgg ggctccgttg tcgccgcggt ggtcaaaacc
    15901 gtcaccaaaa tcctcggcgc tatggctgac ctggaactga taattcaggc catcgacgat
    15961 cttgccgtag aagccggctg atccggccac ccaggtcgtc gggatcaggc cgttggcgag
    16021 ctccggacgt tccacgctgt agaacagcgt cggttcgtgg tagaggttgt tgaacgagat
    16081 cggcaccaga tcgacgccgg gcgagcgcaa ggtgaggtga tcgttgaccc tgaaatcgaa
    16141 gaaggcctgt tcgatctcgg cggcgccgtg cagcttgtcg tcgttgtcaa acgcggaccc
    16201 ggcgtgctcg aactcgatct cggcgttgaa gatgatgttg tcggtgacct gataggtcgg
    16261 caacaacacc aggcgtccca tatcgaagcc gttctgccac tgaccattgt gcgcaggatt
    16321 ctgctgcgag ccatacttga tctcgccgta catgccgatc ttgagcccgg gaaccggggc
    16381 ttgcgccacc ccgaacaggt cattgttgtt cttgttcggc gaagccgccg gcgcatcggc
    16441 agcgggtgcc tgggcgtatg ggcccgtcgg cttgcccgcg acagtcggcc gaccggcaga
    16501 cagcgactga gccgtccgcg gggtttgctc ctgcttctgc ttgagctcgg actcgagtgt
    16561 ctgcatgcgc ttttccatgg cctggagctt cttcaacagc atctcgttcg tcgccttgga
    16621 tgcgctgtcg tcgccggcga gcaccgccgc gctgctcccg ccgagcatca acgaagcaat
    16681 cagcactgtc gacgtggagc ggcccatgcc ttgagcggtt gcgctgccga gcttccgctc
    16741 gaagcgagac gcctgaccgc tcgaagacat aagcaaatgc ggcagaaagc agtcacggat
    16801 ggacataagc aaggactccc ccaggttaaa agcgatcgag gaaaccccat cgtccccacg
    16861 aagcgcgtca tcgcatcaac ttcgttcagc cataggttcg tcatcccatc gtgtcaattt
    16921 cggcaacgcg catcgagcct gcactacgcg gctctgccac ctcaatcaca tcaatgcggt
    16981 gcctccatga gttgtgcagc ggatcggaac gatggcgccc gcgcatgctc cgatatgagt
    17041 caataagatt cggggcagac tgttcttaaa gacctttcac cgacattcgc gagcagacgc
    17101 gtcatcaatt acgtcgcgat ttttttgcaa ccccagatta tttgtttctg aaacatcaca
    17161 cttgttggaa tttcaaggag cccatgagtg gatcagcgcc ggactcttca tcgcgatcga
    17221 cactgcacag gaaaaacaga attggctcac aggaagaaca gaattgactc acacgaaaaa
    17281 cagattgcgt ttccgatgtt cgtctcgaca gcgccacact ggcgccaacg cgatgccctg
    17341 agttcaactg aaatgcgatc caccgggctc tgcgaggagt tgggccgtcc gcgacgagtg
    17401 gcagtgatcg cccggcacaa cctgcgagcg gtttggacgc gggctcaact cgcaatagca
    17461 caggaaagga tcgatattcg ccgcgcaaaa gcggccgacg ctaccgacag tccatagcgg
    17521 actgcgctgg aggcggttcg attgaatcga aacccatccc ggaattcgtc attgtctcaa
    17581 aaggggaagc gtctcaagga agacgagcgt cggccgcgtc tgcggccggc gctgcgagtg
    17641 gtcacgaatg atgatggtgg tgatggtgat gatggtggtg gtggtggtgc ttttctttca
    17701 ccatcattgc gtgcggctcc ggcgtcgccg cggccccttt cacggtgacg gtgatgacct
    17761 cggattggac cggcggcttg aacggaatat gggtccagtc gccgagcatg agctgcagcg
    17821 tgtgcttgcc gggcggcaac gtcacagtgg tctcggtctg gcctttgccg aagtgaacat
    17881 gctgctcgtc gaccataatg ggttgggtca tttcctcagg cgtgagcgcc ttgtcgatga
    17941 gcaggtgatg atgcccgacg tttgccttct cgacgccgga aggagcgacg cccatgttcg
    18001 ggctcagtcc gaacaccacc ttgaacgggc tggtgacggt atcgccgttc ttgaggttca
    18061 cgatatacgc gaacgcgccg gggggtgagt tcgattgccc cgagtctgat ggctgcgctg
    18121 ctgtggccgg gggcgccggc ggctgttgcg ccgatgccgg cccgatggca gccgcaatcg
    18181 ttgcaacggt caggacgccg tagcagaatt tgaacatggg tagcctttcg aaaaaaaggg
    18241 gaagtggagg aggttggaaa gcgggccgcg gggtgacttg acgccgtcag tgacgcggct
    18301 ctgggtgcgt cgtcatccct gaccagggac gtcccggtga gagcgcgctg caatcccatg
    18361 gccggccact gctcgccata tttggataca agatgtcaaa cggggccaaa gcgtgacaga
    18421 ttccgcgcat tgataaagat ctattcaagc gctatccgat cgatcgttat ggaatagcag
    18481 atcggcgcat cgtgccaggt ttatgatcaa cacaagcgtc ttgatcgtgt catcaaatat
    18541 tcacggccgg gcgcgg
//
LOCUS       DATNOI010000476         7109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_730741,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000476 DATNOI010000000
VERSION     DATNOI010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730741"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VN968_19005"
     CDS             complement(<1..321)
                     /locus_tag="VN968_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HXL31359.1"
                     /translation="MLNRDQDILKAAEDWQKAGHGVALATVVETWGSAPRPAGSSLVI
                     NDDGTFLGSVSGGCVEGAVVTEALDVIASGQPKMLEFGVADETAWNVGLSCGGTIKVF
                     VEKVG"
     gene            complement(334..1533)
                     /locus_tag="VN968_19010"
     CDS             complement(334..1533)
                     /locus_tag="VN968_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXL31360.1"
                     /translation="MTTSTTTPTTGLIADNVIGFARALRAAGIPVGPGAVIDALKALR
                     VIDIGNRADVFTTLESIFVKRHEHALIFAHAFDLFFRAAEDWKHMLDSVPLPEHARKK
                     PPPASRRVQEALSHPHMTETPQAREQELRLSVSDQEVLQKKDFAQMSAAEIAQATRAI
                     AQMKLPQAELRTRRYQPDARGLRLDMRRTLRGSLRTGGDIIDIHRLGRIDKPAPVVAL
                     LDISGSMSEYTRLFLHFLHAITDARKRVSVFLFGTRLTNVTRALRSRDPDEALARCSS
                     AVQDWAGGTRIATSLHTFNKLWGRRVLGQGAIVLLISDGLEREADARLAFEMDRLHRS
                     CRRLIWLNPLLRYGGFEARAQGIKMMLPHVDEFRPVHNLSSIEGLVEALSSASPTHHR
                     GLIRSAA"
     gene            complement(1764..2672)
                     /locus_tag="VN968_19015"
     CDS             complement(1764..2672)
                     /locus_tag="VN968_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXL31361.1"
                     /translation="MNASALPRSVDAMLEMLTSRGYLAERSLGTVTYLSLRMGRPLFL
                     EGEAGVGKTEIAKVLAAALKRKLIRLQCYEGLDVASAVYEWNSAAQMIAIRLAEAAGD
                     TDRDQLSSDIFAERYLIKRPLLQALEPDVAGAPVLLIDELDRADEAFEAYLLEILSDF
                     QVTIPELGTVKAPQPPIVVITSNRTREIHDALKRRCLYHWVDYPSAERELAIVKSRVP
                     GISAKLSQQVVGFVQALRQQDFYKSPGVAETIDWATALTELDARSLTPQVVGDTLGAL
                     LKYQDDIARMQGDTLQKVLKEAESEN"
     gene            complement(2710..3516)
                     /locus_tag="VN968_19020"
     CDS             complement(2710..3516)
                     /locus_tag="VN968_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXL31362.1"
                     /translation="MYEFKYHRPATVRQAANLLVKNEDAKVIAGGHTLLPVMKQRLAS
                     PPHLVDLSHIEGLDSIEMKGRSLVIGATAKHADVAASAVVGEAIPALAELAGMIGDPA
                     VRHKGTIGGSLANNDPTADYPAAVMALGATIVTNKRRLKPEEFFQGLFTTALENDEII
                     TRVMFPVPKKAAYVKFRNQASRYALVGVFVAKRPSDVRVAVTGAGSEGVFRLTAFEEA
                     LKKRFSHKVLDGLTVPAEGLNSDLHGSAEYRAHLIAVLARRAVEAANAKA"
     gene            complement(3549..4034)
                     /locus_tag="VN968_19025"
     CDS             complement(3549..4034)
                     /locus_tag="VN968_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL31363.1"
                     /translation="MAKISLIVNGNPVNANVDSRTLLVQFLRENLRLTGTHVGCDTSQ
                     CGACVVHLDGKAVKSCTTLVVMADGHEVKTIEGLAADGAPLHPMQEAFREHHGLQCGF
                     CTPGMIMTAVDIVHRKGHDLSEHTIREELEGNLCRCTGYQNIVQSIAAGAKAMANSDL
                     A"
     gene            complement(4227..4673)
                     /locus_tag="VN968_19030"
     CDS             complement(4227..4673)
                     /locus_tag="VN968_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HXL31364.1"
                     /translation="MAMTMNGEVQLAAPREVVWSKLNDPEVLKACIPGCEELEKTEET
                     GFRAVAKMKVGPVSARFRGRVTLSDLDPPNGYKISGEGEGGVAGFAKGGATVALAEKD
                     DGTLLTYDVEAQIGGKLAQLGQRLINGAAKKLADEFFSNFAKAVQG"
     gene            4853..6208
                     /locus_tag="VN968_19035"
     CDS             4853..6208
                     /locus_tag="VN968_19035"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HXL31365.1"
                     /translation="MSTALSPLLAPMLSSAAMRAVCDDGAYLQHMLDFEAALARAEAA
                     AGVIPGNAAGPIAKACKASSFDLADLAEAATRSGNLAIPLVKALTANVAKADADAARH
                     VHWGATSQDVIDSAGMLTLRAAIDALLPDLDRAIAGFARLARQHRNTAVVARTWLQHA
                     LPMPFGLKLAEYAAALHRSRARLRRLRNEGLALQFGGAAGTLAAIGDKAMVVAEKLAQ
                     ELKLPLPEAPWHTHRDRIAEAASVFAILTGTCGKIARDVSLMMQTDVAEAFEPSGEGR
                     GGSSTMPHKRNPVAAAAALAAATMAPNLAATIFAAQVQDHERSAGPWQAEWPTLPSLL
                     LVTSGALVAIVDIAEGLQVDAARMRANLEATGGLIMAEAVAMALAEMIGKSEAHHLVE
                     AASKKAVAEKKGLRDVLTKNPKVTAHLSADRLAKLFEPMAYQGASQALIDRLLASLDD
                     K"
     gene            6339..>7109
                     /gene="pcaD"
                     /locus_tag="VN968_19040"
     CDS             6339..>7109
                     /gene="pcaD"
                     /locus_tag="VN968_19040"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HXL31366.1"
                     /translation="MPMIDADGCLLNVSVEGRDGGPTLMLSNSLGCTLQMWEPQMKAL
                     TQLFRVIRYDRRGHGKSSVPPGPYSMERFGRDVLAILDDLNIEKVHWCGLSMGGMVGQ
                     WLGANAPERLGKIILSNTACYYPDPTNWRNRIKAVKEGGIAAVADTVIASWLSADFRE
                     REPQIAAKMKAMLIASPVQGYLACCEALSTLDQRELLPRIKSPTLVIAGRHDTATPIA
                     AGELIRSRIPGASMTILDAAHISNVEQPHAFTDAVIGFL"
BASE COUNT         1279 a         2293 c         2257 g         1280 t
ORIGIN      
        1 cccgactttc tcgacaaaca ccttgatggt gccgccgcag gacaggccga cgttccacgc
       61 ggtctcgtcg gcgacgccga attcgagcat tttcggctgc ccgctggcga tcacatcgag
      121 cgcctcggtg acgacagcgc cttcgacgca gccgccggac accgatccca gaaacgtgcc
      181 gtcatcgttg atcacgaggc tcgaacccgc gggccgcggc gccgagcccc aggtctcgac
      241 cacggtcgcc agcgccacgc catgaccggc cttctgccag tcctcggccg ctttcaagat
      301 gtcctggtcg cgattgagca tggaaaacct ctttcaagct gcggatcgaa tcaggccgcg
      361 gtgatgggtc ggcgatgccg acgaaagtgc ctcaaccagc ccctcgatcg aactcaaatt
      421 atgtaccgga cggaattcgt caacgtgcgg cagcatcatt ttgatgccct gcgccctggc
      481 ctcgaagccg ccatagcgca acagcgggtt gagccagatc aggcggcggc acgacctgtg
      541 cagccggtcc atctcgaatg ccagcctggc gtcggcctcg cgctccagcc cgtcggagat
      601 cagaagcacg atggcgccct gcccgagcac gcgccggccc cacaacttgt tgaaggtatg
      661 cagcgaggtc gcgatccggg tgccgccggc ccagtcctgc actgccgacg aacagcgtgc
      721 cagcgcctcg tcaggatcgc gcgaccgcag cgcccgcgtc acattggtca gtcttgtgcc
      781 gaacaggaac accgagacac gcttgcgcgc gtcggtgatg gcgtgcagga aatgcaggaa
      841 cagccgggtg tattcgctca tcgagccgga tatatcgagc agcgccacga ccggggccgg
      901 cttgtcgatc cggcccaacc tgtggatatc gatgatgtcg ccgccggtgc gcaaggatcc
      961 gcgcagggtg cggcgcatgt cgagccgcaa gccccgcgca tcgggctgat agcggcgggt
     1021 gcgcaactcg gcctgcggca acttcatctg cgcgatggcg cgcgtcgcct gcgcgatctc
     1081 ggccgcgctc atctgcgcga aatccttctt ctgcagcact tcctggtcgg acaccgagag
     1141 gcggagctcc tgctcccgag cctgcggcgt ttcggtcatg tgggggtgcg acagcgcctc
     1201 ctggacgcgg cgcgaagccg gcggcggctt tttcctggca tgctccggca gcggcaccga
     1261 atccagcatg tgcttccagt cctcggcggc gcggaagaac agatcgaagg catgggcgaa
     1321 aatcagcgca tgctcgtggc gcttgacgaa gatcgactcc agcgtggtga agacgtcggc
     1381 gcggttgccg atatcgatca cccgcagcgc cttcagtgcg tcgatcacag caccagggcc
     1441 aaccggaatc ccggcggcgc gcagcgcgcg ggcaaagccg atgacgttgt cggcgatcag
     1501 gccggtggtg ggcgtggtgg ttgaggtggt cattcaaaaa cgaatcccgg tcgcgatgtc
     1561 tcggcctctg tcacctctcc ccgttcttta cggggagagg tcgaaattct cgcgacgcga
     1621 gaatttcggg tgaggggtga ggcacagcgt caactcgttc acgccggcgc aaggtgggag
     1681 aagggcccct caccccaacc ctctccccgc aagagcgggg cgagggagct gaccgccgct
     1741 gcggcgcgtt ccatggtcga caactaattc tcgctctccg cctctttcaa aaccttctgc
     1801 agcgtgtcgc cctgcatccg cgcgatgtca tcctggtatt tcaacagcgc gccgagcgta
     1861 tctcccacca cctgcggcgt cagcgagcgg gcgtcaagct cggtcagcgc ggtggcccag
     1921 tcgatggtct cggcgacgcc cggcgacttg tagaaatcct gctggcgcag cgcctgcacg
     1981 aagccgacca cctgctgcga caattttgcg gagatacccg gcacgcgcga tttgacgatc
     2041 gccaactcgc gctcggcgct tggatagtcc acccagtgat agagacagcg gcgcttcagc
     2101 gcgtcgtgaa tttcgcgggt gcggttcgaa gtgatgacga cgatcggcgg ctgcggcgcc
     2161 ttgacggtgc cgagttcggg gatcgtgacc tggaagtcgg agagaatttc cagaagatag
     2221 gcctcgaatg cctcgtcggc gcggtcgagc tcgtcgatca gcaacaccgg cgcgcccgcc
     2281 acatcgggct ccagcgcctg caagagcggc cgcttgatca gatagcgctc ggcgaaaata
     2341 tccgacgaca actggtcgcg gtcggtatcg ccggccgctt ccgcgagccg gatcgcaatc
     2401 atctgcgccg cgctgttcca ctcgtagacc gcggaggcaa cgtccaggcc ttcatagcac
     2461 tgcagccgga tcagctttcg tttgagcgcc gccgccagca cttttgcgat ctcggtcttg
     2521 ccgacgccgg cctcgccttc gagaaacagc ggccggccca tgcgcagcga cagatacgtc
     2581 accgtcccca gcgagcgctc ggcgagatag ccgcgcgagg tcagcatttc gagcattgca
     2641 tcgacagacc tgggcagcgc cgatgcgttc atgggaagac caatcttgat gcagcgagac
     2701 gtggcaatgt cacgctttgg cgttcgcggc ctcgacggcg cgccgcgcca gcaccgcgat
     2761 cagatgcgcg cggtattcgg cgctgccgtg caggtcgctg ttcagtccct cggcgggcac
     2821 ggtcaggccg tcgagcacct tgtgcgagaa acgcttcttc aaagcctctt cgaacgcggt
     2881 caggcggaac acgccctcgg agccggcacc ggtgacggcg acgcgcacgt ccgacggacg
     2941 cttggcgacg aaaaccccga ccagcgcgta gcgcgaagcc tggttgcgga atttgacgta
     3001 agcggccttc ttcggcaccg ggaacatcac cctggtgatg atctcgtcat tctcaagtgc
     3061 cgtcgtgaac agcccctgga agaattcttc cggcttcaac cggcgcttgt tggtgacgat
     3121 ggtggcgccg agcgccatga ccgccgcggg ataatcggcg gtcggatcgt tgttggcgag
     3181 cgaaccgccg atggtgccct tgtggcgcac cgcgggatcg ccgatcatgc ctgcgagctc
     3241 ggccagcgcc gggatcgcct cgccgacaac cgccgaagcc gcgacatcgg catgtttcgc
     3301 ggtggcgccg atcaccagcg agcggccctt catctcgatc gagtcgaggc cctcgatatg
     3361 ggagaggtcg accaggtgcg gcgggctggc gagtcgctgc ttcatgaccg gcaacagggt
     3421 atggccgccg gcgatcacct tggcgtcttc attcttgacc aggagattgg ctgcctgccg
     3481 cacggttgcc ggacgatgat atttgaattc gtacatgggc tatccctaac cgcggtgcgg
     3541 tgaatcaact atgcgagatc ggagttcgcc atcgccttgg cgcccgccgc gatcgattga
     3601 acgatgttct gataaccggt gcagcggcag agattgcctt cgagctcttc gcgaatggta
     3661 tgctcggaga gatcgtgacc cttgcggtgg acgatatcaa ccgcggtcat gatcatgccg
     3721 ggcgtgcaga agccgcattg caggccgtga tgctcgcgga atgcctcctg catcggatgc
     3781 agcggcgcac cgtcagccgc aaggccctcg atggtcttga cctcgtgccc gtcagccatc
     3841 accacgagcg tggtgcagga tttgaccgcc ttgccgtcga gatgcacgac gcaggcgccg
     3901 cattgcgagg tgtcgcagcc gacatgggtg ccggtcagcc gcagattctc gcgcagaaac
     3961 tgcaccagca gtgtgcggga atcgacattg gcattcacgg gattgccgtt cacgatcagg
     4021 gaaatcttgg ccattcagca ctctcttctt ctgcgctgct gcattgcaca gaagcacagt
     4081 ctttaaaatc attgcaaagc cataatatgg gccatcccgg caatgagcaa cctcacctgg
     4141 ggtgtcgcct gatatgactt gttgggactt cggtagcgat cagccttgtt agggatcagc
     4201 tttgttgggg attagcctgt cagggattag ccttgcaccg ccttggcgaa attggagaaa
     4261 aattcatcgg ccagtttctt ggccgcaccg ttgatcaacc gctgcccgag ctgcgccagc
     4321 ttgccgccga tctgcgcctc gacgtcgtaa gtcagcaacg tgccgtcgtc cttttcggcc
     4381 agcgccacgg tcgcaccacc cttggcgaag cccgcgacac cgccctcgcc ttcgcctgat
     4441 atcttgtagc cattcggcgg gtcgagatcg ctcagcgtga cccggcccct gaagcgggcc
     4501 gacaccggcc cgaccttcat cttggcgacg gcacgaaaac cagtttcctc ggttttctcc
     4561 agctcctcgc atccggggat gcaggccttg agcacttccg gatcgttcaa cttgctccac
     4621 acgacctcgc gcggcgccgc aagctggact tcgccgttca ttgtcatggc catgggtcgc
     4681 ctccaccggt cgcgtctttt cgttctctca agtaaagcac gcaagcccca aaaggaaggg
     4741 ggacatcggt ccacgagcaa tgcatattcg caagccgcgg acctgtcagc ggctcgggaa
     4801 tacggctttg ggattggcag gggcgaaaca ggatggttag gtcgcgtgca tcatgagcac
     4861 tgctctctcc ccgttgctcg ctcccatgct gtcgagcgcc gccatgcgcg cggtctgcga
     4921 tgatggagcc tatctccagc acatgctgga tttcgaagca gcgctggcgc gcgcggaagc
     4981 cgcggccggc gtcatccccg gaaatgcagc cggcccgatc gcgaaggcct gcaaggcaag
     5041 ctcgttcgat ctcgccgatc tggccgaggc cgcgacacgt tccggcaatc tcgcgatccc
     5101 cctggtcaag gcgctcaccg ccaatgtcgc caaggccgat gcggatgccg cgcgccacgt
     5161 gcattggggc gccaccagcc aggacgtgat cgatagcgcc ggcatgctga cgttgcgcgc
     5221 ggccatcgac gcacttctgc ccgatctcga tcgcgccatc gcgggctttg ccaggctggc
     5281 acggcagcat cgcaacaccg ccgtggttgc gcgcacctgg ctgcagcatg cattgccgat
     5341 gccgttcggg ttaaagctcg cggaatatgc cgccgcattg catcgctcgc gcgcgcggct
     5401 gagacgactg cgcaacgagg gattggcgct gcagtttggc ggtgccgcgg ggacgttggc
     5461 agcaatcggc gacaaggcga tggtcgtcgc ggagaaactg gcgcaggagc tgaagctgcc
     5521 gctgccggag gcgccgtggc acacccatcg cgaccgaatc gccgaggcgg cctcggtatt
     5581 tgcgattctc acgggcacct gtggcaagat cgcccgcgac gtttcgctga tgatgcagac
     5641 cgacgtcgcg gaagcattcg agccgtcggg cgaaggccgc ggcggttcgt ccaccatgcc
     5701 gcacaagcgc aacccggtcg ctgccgcggc ggcgctcgcc gcggcgacca tggcgccgaa
     5761 cctggcggcg accatttttg cggcccaagt gcaggaccac gagcgcagcg ccggaccatg
     5821 gcaggcggaa tggccgacct tgccaagctt gctgctggta acctcgggcg cgctcgtggc
     5881 catcgttgac atcgccgaag ggctgcaagt cgatgctgcg cgcatgcgcg ccaatctcga
     5941 ggcgacgggc gggctgatca tggcggaagc cgtggcgatg gcgctcgccg aaatgatcgg
     6001 caagagcgag gcccatcatc tggtggaggc cgcgagcaaa aaagccgtcg ccgagaaaaa
     6061 aggcctgcgt gatgtcctga caaagaaccc gaaggtcacc gctcatctga gcgcggacag
     6121 gctggcaaaa ctgttcgagc cgatggccta tcagggcgcc tcgcaagccc tgatcgaccg
     6181 cctgctcgcc tcgctcgacg ataaatagtc cggatgtcgt tgccgggctt gacccggcaa
     6241 tccatcttct tgaatagact tctcttcgat ggatgcgcgg gtcatctggc ctgaatacgc
     6301 gcgtcgcgct ttagcccgcg cgtgatgagg aacataaaat gccgatgatc gatgccgacg
     6361 gttgcctgct caacgtctcc gtcgaaggcc gcgacggcgg gccgaccctg atgctgtcga
     6421 attcgctggg ctgcaccttg cagatgtggg agccgcagat gaaggcgctc acccagcttt
     6481 ttcgggtgat ccgctatgac cgccgcggcc atggcaagtc cagcgtgccg cccggcccct
     6541 attccatgga acgtttcggc cgcgacgtgc tggcgatcct cgacgacctc aacatcgaga
     6601 aggtgcactg gtgcggactg tcgatgggcg gcatggtcgg gcaatggctc ggcgccaacg
     6661 caccggagcg gctaggcaag atcattcttt ccaacaccgc ctgctactac cccgatccga
     6721 ccaactggcg caaccgcatc aaggcggtga aggaaggcgg catcgcggcg gtggccgaca
     6781 ccgtgattgc aagctggctg agcgcggact tccgcgagcg cgagccgcag atcgcagcga
     6841 agatgaaggc gatgctgatc gcgtctccgg tgcagggcta tcttgcctgc tgcgaagcgc
     6901 tgagcacgct cgatcagcgc gaactgttgc ccaggatcaa aagcccgacg ctggtgattg
     6961 ccggtcgtca tgacacggcg acgccgatcg cggccggcga attgatccgc agccggattc
     7021 ccggcgcgag catgacgatt ctcgacgccg cacatatttc caatgtcgag cagccgcacg
     7081 ctttcaccga tgccgtgatc ggcttcctg
//
LOCUS       DATNOI010000477         8173 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1762521,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000477 DATNOI010000000
VERSION     DATNOI010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8173
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762521"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VN968_19045"
     CDS             <1..208
                     /locus_tag="VN968_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL31367.1"
                     /translation="TAILRFLYRAGQLPVSRETLLQEVWGYNSGVTTHTLETHIYRLR
                     QKIEKDAANPEILVTEAGGYKLVP"
     gene            217..402
                     /locus_tag="VN968_19050"
     CDS             217..402
                     /locus_tag="VN968_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31368.1"
                     /translation="MVLRIRSLPGAAAIAWAKFGSEDTVSHDYSAILVQKFSQRSLDV
                     LLEDLRRGRSELLHRRI"
     gene            421..864
                     /locus_tag="VN968_19055"
     CDS             421..864
                     /locus_tag="VN968_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXL31369.1"
                     /translation="MSIEDDVALLERVPTLRLLGTAALRMLAIGSEQRNVARGDLLFN
                     EGDDADAGFIVQRGTLRVEDRAGAEIVVGPGALIGELALVVAMRRPCTATALEHCSMI
                     RITRSLFQRVLESDPAAAQRLRDEFTSRTSQVASDILMAGAKLSI"
     gene            complement(930..1745)
                     /locus_tag="VN968_19060"
     CDS             complement(930..1745)
                     /locus_tag="VN968_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXL31370.1"
                     /translation="MRFAVTTWNINSVRLRIDLVAKFLKSVRPDVLCLQETKCVDGAF
                     PLKRFRRLGYEHVALNGQKGYHGVAVISKLPFETTDIRTFCDKIDSRHISVQFGEKAR
                     LSKPLVLHNFYVPAGGDIPDPALNAKFEHKLQFLDEMKACEPLHPRGEDIHILVGDLN
                     VAPHENDVWSHKQLQKVVSHTPIECEKLLAAQTHGEWIDVARERIPLSEKVYTWWSYR
                     AADWTLADRGRRLDHIWVSRALKERVSDFRITRDARGWERPSDHVPVTVTLEV"
     gene            complement(1840..2706)
                     /locus_tag="VN968_19065"
     CDS             complement(1840..2706)
                     /locus_tag="VN968_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HXL31371.1"
                     /translation="MAREFAVPGLRTALAVLIAAAVASGSTPSAAQLFSTTPSPPPVA
                     VPPPVAVPMPKPAPKARRLQMTASDPPKAQMTTGATQAAPPNPVIPDPRRNIPANLFA
                     TFDANQKAQAAKVSSYLSSLQTLVGNFVQVGPDGSKTKGDFYIQKPGKVRFEYDDPSP
                     IAIIADGSSLAVRDRKLATQDIYPLSQTPLRFLLSDRIDLLKDTNVVAVTSDEVYISV
                     TIEEKQALIGTSRLMLMIGTKDGQLKQWTVTDPQGYDTTVAVYNLDANKKVDPGLFKI
                     DFTTYPGGTPPG"
     gene            complement(2844..5276)
                     /locus_tag="VN968_19070"
     CDS             complement(2844..5276)
                     /locus_tag="VN968_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HXL31372.1"
                     /translation="MSMPAIERVIPLVGHLPASIREALARRLRELTGLALLSLSGIGA
                     AALMTWSVQDPSLSHATSRAIRNIVGYPGAIFADLLMQILGLGAIMLILPVAVRGWRM
                     LTHRTFDREALRLACWILCAVISAGFASCWPHGGAWPLPTGLGGVVGDALVRAPAVVF
                     GPPGIVYRLVLGVILFAAAAATLLIAGGMGSRPQEDELTPIEDDDAPFEEEEDDGSVS
                     LGWAFHAAMSAKARLWRLLTLAYSRLVASAPPQRASFERQEPNLGGRAAQPSTPHAAD
                     DSDEDEDEDEEIPARAPRKKAAPKAASRKAWFELPPISVLTAPKASDRQPLSKSDLDT
                     NSRSLEGVLGDFGVRGEIVKAHPGPVVTLYELEPAPGIKSSRVIGLADDIARSMSALS
                     ARVAVVPGRNAIGIELPNPHREKVYLRELLAAKDGNESTAKLPLCLGKNIGGESIIVD
                     LARMPHLLIAGTTGSGKSVAINTMILSLVYRLRPDQCRLIMVDPKMLELSVYDGIPHL
                     LTPVVTDPKKAVVALKWAVREMEERYKKMSKLGVRNIDGYNARLVEAKGKGEELTRTV
                     HTGFDKETGKAIYEEEKLDLEPLPYIVIIVDEMADLMMVAGKDIEGAVQRLAQMARAA
                     GLHVILATQRPSVDVITGTIKANFPTRISFQVTSKIDSRTILGEMGAEQLLGQGDMLY
                     MAGGGRISRVHGPFVSDEEVEKVVRHLKAQGQPEYLEAVTAEEPTDEDGAVFDSTGMG
                     GDGGGDLFSQAVAIVKRDRKASTSYIQRRLQIGYNRAASLMERMELEGIVGQPNHAGK
                     REILVEEEEGGF"
     gene            complement(5289..6503)
                     /locus_tag="VN968_19075"
     CDS             complement(5289..6503)
                     /locus_tag="VN968_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXL31373.1"
                     /translation="MAMTASSRAPQGSGNQESERSPFARLTELLAPYQPAKPLITLSL
                     GEPQHPVPAFVGPVLANHIGDFGRYPIAKGIEPFRRAAANWLSTRFNLPRGVDPETEL
                     LVLNGSREGLFFAALTAARYAGERKGRPAILIPNPFYPAYAAGARTAGCEAIYLPTTL
                     ANGFLPDLDALDDATLARTVAIFIASPANPQGAVASRGYFARLKRLADRFGFMILSDE
                     CYSEIYTRQAPGSMLECAGPDFTNVVAFQSLSKRSNLPGMRVGFAAGDRKFLGMFHEL
                     RNVAAPQVPVPLQHVAVAAYSDEAHVEENRRLYRIKFDLADQILGNRYGYDRPAGGFC
                     VWLDVSARGGDEAATVRLYKDGGVRVVPGSYLARRQADGSNPGAGYIRLALVQDSETT
                     AEALHRLVEILD"
     gene            6958..7941
                     /locus_tag="VN968_19080"
     CDS             6958..7941
                     /locus_tag="VN968_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004673375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL31374.1"
                     /translation="MKVLVTGSAGHLGEALVRTLTNMQYEVVGLDILESPFTTRVGSI
                     TDRSCVRHCMHGVQAVFHAATLHKPHIATHPRQDFVDTNVTGTLNLLEEAAAAGVVSF
                     VYTSTTSIFGDALVPPPGAPATWVTEEVAPVPKNIYGVTKAAAEDLCQLFHRNQALPC
                     IVLRTSRFFPEEDDNKDVREAYSDDNVKVNEYLYRRVEIEDVVSAHLLASERAPAIGF
                     RKYIVSATTPFLPDDLPALRVDAPRVVRLRVPEYEAEYERRGWKMFPSIGRVYVNDRA
                     QRELGWQPRYCFNDIIGRLKAGDDLRSPLARVTGSKGYHAEPFPDGPYPVE"
BASE COUNT         1538 a         2535 c         2534 g         1566 t
ORIGIN      
        1 aacggcgatc ctgcggtttc tgtaccgggc cggccagctg ccggtatcgc gcgagacgct
       61 gctgcaggaa gtatggggct ataattcagg cgtcaccacc cacaccctgg aaacccacat
      121 ttaccggctg cgtcagaaga tcgagaaaga tgcagccaat cccgagatat tggtaacgga
      181 agccgggggt tacaagctgg tcccctgata cttcacatgg tgcttcggat ccgaagtttg
      241 cccggtgcgg cggcgattgc ttgggcgaaa ttcggttctg aagacaccgt cagccatgat
      301 tacagtgcga ttttggttca gaagttttct caacgttctc ttgacgtttt gctggaggac
      361 ttgcggcggg gacgaagcga acttctgcac cgccgcatct agcccgatca ccggttctgc
      421 atgtcgatcg aagatgatgt tgccctgctc gagcgcgtcc cgacattgcg cctgttgggg
      481 acggcagcgc tgcgcatgct ggcgatcggc tccgagcagc gcaatgtcgc gcggggcgat
      541 ctgctcttca atgaaggcga tgatgcggac gcgggattta ttgttcagcg cggcacactc
      601 cgggtcgagg atcgcgctgg cgccgagatc gtcgtcggcc caggcgcatt gatcggcgag
      661 cttgcgcttg tcgtcgccat gcggcggccc tgcaccgcaa cggcgctgga acactgctcg
      721 atgatccgca tcacgcgcag cctgtttcag cgcgtgctgg aaagcgatcc cgccgccgcg
      781 caacggctgc gcgacgaatt caccagccgc accagccagg tcgccagcga tatcctgatg
      841 gccggcgcga agctgagtat ttgacccagc cattcgtcat tccgggctcg ctcgtttcac
      901 gcgccccgga atgacgagga tttatttact cacacctcca gcgtcaccgt caccggcacg
      961 tggtcggacg gccgctccca gccgcgcgca tcccgcgtga tccggaaatc gctgacacgc
     1021 tctttaaggg cgcgcgacac ccagatgtga tcgagccggc ggccgcgatc ggccagcgtc
     1081 cagtccgcgg cgcggtaact ccaccacgta tagacttttt ccgagagcgg gatgcgctcg
     1141 cgcgcgacat cgatccattc accgtgagtc tgcgccgcca gcaacttctc gcattcgatc
     1201 ggggtgtgcg acacgacttt ttgtaattgc ttgtgcgacc acacgtcatt ctcatgcggt
     1261 gccacattga gatcgccgac caggatatgg atgtcttcgc cgcgcggatg caacggctcg
     1321 cacgccttca tttcgtcgag gaattgaagc ttgtgctcga acttcgcatt gagggcggga
     1381 tccggaatat cgccgccggc cggaacatag aaattatgca gcaccagcgg ctttgacagc
     1441 cgcgcctttt cgccgaactg aaccgagatg tggcgggaat cgatcttgtc gcagaaggtg
     1501 cggatgtccg tggtctcgaa cggcagtttc gagatcaccg ccacgccgtg ataacccttc
     1561 tgcccgttca gcgcgacgtg ctcatagcca aggcgcctga accgcttcag cggaaatgcg
     1621 ccatcgacgc atttggtttc ctgcaggcac agcacgtccg gccgcaccga tttgagaaat
     1681 ttggcgacga ggtcgatgcg caggcgcacc gaattgatat tccacgttgt cacggcgaag
     1741 cgcatctctg agggggcctt gcgacgcgag gatacgctca tccggagatg acgctgtcca
     1801 ctgtgaagcg cgatcaggag atgcaatcaa gaacgatttt cagcccggtg gcgtcccccc
     1861 ggggtaggtg gtgaagtcga tcttgaacag gccgggatca actttcttgt tggcgtccag
     1921 attgtagacc gcaaccgtgg tgtcgtagcc ttgcggatcg gtgaccgtcc attgcttgag
     1981 ctgaccgtcc ttggtgccga tcatcaacat caggcggctg gtgccgatca gcgcctgctt
     2041 ctcctcgatg gtcacgctga tgtagacctc atccgaggtc acggccacga cgttggtgtc
     2101 cttcaagaga tcgatgcgat ccgacagcag aaaccgcagc ggcgtctgcg acaacggata
     2161 gatgtcctgg gtcgcaagct tgcggtcgcg caccgccagc gacgatccgt cggcgataat
     2221 cgcgatcggg ctggggtcgt catattcgaa acgcaccttg ccaggcttct ggatgtagaa
     2281 atcgcccttg gtcttgctgc cgtcgggccc gacctggacg aaatttccga ccagcgtttg
     2341 cagcgacgac agataggaac tcaccttggc ggcctgcgcc ttttgattcg catcgaaggt
     2401 cgcaaaaaga ttggcgggaa tattcctgcg cggatcggga atcaccggat tcggcggggc
     2461 agcctgggtt gctccggtcg tcatctgggc cttcggcgga tcggacgcgg tcatctggag
     2521 acggcgggct tttggcgccg gttttggcat cggcacggcc acgggcggcg gcacggccac
     2581 gggcggcgga gatggcgtcg tcgagaagag ctgcgcggca gacggggtcg acccgctcgc
     2641 cacggccgcc gcgatcagca ccgccaaagc cgtgcggagg cccggaaccg cgaattctct
     2701 tgccactcga attttcggat tctgaatcaa cgcgtcgtcc taccctgcgc ttcgcccttt
     2761 tatcgtgctt tcggcaaaag gagatatcgt ttttccgtga aaaataatgc caattccggc
     2821 aatcacttgg ctggtgatgc tgttcaaaaa ccgccttctt cttcctcgac caggatctcg
     2881 cgtttgccgg catgattcgg ctgcccgacg atgccctcca gttccatccg ctccatcagc
     2941 gaggcggcgc ggttatagcc gatttgcagc cggcgctgaa tgtagctggt ggacgccttg
     3001 cggtcgcgct tgacgatcgc cacggcctgc gaaaacaggt cgccgccacc atcgccgccc
     3061 atgccggtcg aatcgaacac cgcgccgtcc tcgtcggtcg gctcttccgc ggtgaccgct
     3121 tcaaggtatt cgggctggcc ctgggccttg agatggcgca ccaccttctc gacctcctca
     3181 tcggagacaa acggcccgtg cacgcggctg atgcgtccgc cgccggccat atagagcatg
     3241 tcgccctgtc caagcagctg ctcggcgccc atttcgccga ggatggtgcg gctgtcgatt
     3301 ttcgaggtga cctgaaacga gatgcgggtc ggaaagttcg ccttgatggt gccggtgatg
     3361 acgtcgaccg acggtcgttg cgtggcgagg atgacgtgca gaccggcggc gcgcgccatc
     3421 tgcgccaggc gttgcaccgc gccttcgata tccttgccgg cgaccatcat caggtccgcc
     3481 atttcgtcga cgatgatgac aatgtagggc agcggctcga gatcgagttt ctcctcttcg
     3541 tagatcgcct tgccggtttc cttgtcgaaa ccggtatgca ccgtgcgagt cagctcttcg
     3601 cccttgcctt tcgcttccac cagacgcgca ttgtagccgt cgatgttgcg cacgccgagc
     3661 ttggacatct tcttgtagcg ctcttccatc tcgcgcacgg cccacttcag cgcgaccacc
     3721 gctttcttcg ggtcggtcac gaccggcgtc agcaaatgcg gaatgccgtc atagacggag
     3781 agctcgagca tcttcggatc gaccatgatc aaacggcatt gatccggccg caaccggtaa
     3841 accagactga ggatcatggt gttgatggcg accgatttgc cggagccggt ggtgccggcg
     3901 atcagcagat gcggcatccg cgcgagatcg acgatgatgg attcgccgcc gatgttcttg
     3961 ccgagacaaa gcggaagctt tgcagtcgac tcgttgccat ccttggctgc aagcagttcg
     4021 cgcaggtaga ccttttcgcg atgcggattc ggcaattcga tgccgatggc gttgcgtcca
     4081 ggcacgacgg cgacacgcgc cgacagcgcg ctcatcgaac gcgcgatgtc gtcggccagc
     4141 ccgatgacgc gcgacgattt gatgccgggc gcgggttcga gttcgtacag cgtcaccacc
     4201 ggaccgggat gtgccttgac gatttcacca cggacgccga aatcgccgag cacgccttcc
     4261 agcgagcgcg agttggtatc gagatcggac ttgctgagcg gctggcggtc cgaagccttc
     4321 ggcgcggtca gcacggaaat cggcggcagc tcgaaccagg ctttgcgcga tgccgccttt
     4381 ggcgcggcct tcttgcgcgg cgcgcgggcg ggaatttcct cgtcttcgtc ctcatcttcg
     4441 tcggaatcgt ctgcggcatg cggggtcgac ggctgagccg cgcgaccgcc caggttcggc
     4501 tcctgacgtt cgaacgaggc gcgttgcggc ggcgcgctcg cgaccagccg gctgtaagcc
     4561 agggtcagca gccgccacag ccgggccttc gcgctcatcg ccgcatgaaa cgcccatccc
     4621 agcgagaccg agccgtcgtc ctcttcttcc tcgaacggcg cgtcgtcatc ctcgatcggc
     4681 gtcaactcgt cttcctgggg gcgtgaaccc atgccgccag cgatcagcaa tgtcgcggct
     4741 gcggcggcga aaaggatgac cccgagcacg aggcgataga cgatgccggg cggaccaaac
     4801 accacggcgg gcgcgcggac cagcgcgtcg ccgacgacgc cgccaagccc ggtcggcagc
     4861 ggccaggccc cgccatgggg ccagcagctt gcgaagcccg ccgatatcac cgcgcacagg
     4921 atccaacacg cgagccgcag cgcttcgcgg tcgaaggtgc gatgggtcag catgcgccag
     4981 ccccgcaccg cgaccgggag gatcagcatg atcgcgccga ggccgagaat ctgcatcaac
     5041 agatcggcga atatcgcgcc gggatatccc acgatgttgc ggatggcgcg cgacgtcgcg
     5101 tggctcaggc tcggatcctg taccgaccag gtcatcaggg ccgccgcccc aatcccggaa
     5161 agggacaaca aggcgaggcc ggtgagttcg cgcaggcgcc gcgccaatgc ctcgcggatc
     5221 gacgccggca aatggccgac caggggaatg acgcgttcga tcgctggcat gctcatgggg
     5281 ccctgcgatt aatccagaat ttcgaccagc cggtgcaacg cctcggctgt tgtttcactg
     5341 tcctgcacca gcgccagacg tatgtagccg gcgccgggat tgctgccgtc ggcttgccgg
     5401 cgcgccagat aacttccggg caccacgcgc acgccgccgt ccttgtagag tctgaccgtc
     5461 gctgcctcat cgccgccgcg ggccgacacg tcgagccaga cgcagaagcc accggcgggg
     5521 cgatcataac cataacgatt gccgaggatc tggtcggcga gatcgaactt gattcgatag
     5581 agccgccggt tctcctcgac atgggcctcg tcgctgtagg ccgcaaccgc gacatgttgc
     5641 aacggtaccg gcacctgggg tgcggcgaca ttgcgcagtt cgtgaaacat gccgagaaat
     5701 ttccggtcgc cggccgcaaa gccaacccgc atgcccggca ggttcgatcg cttcgacagc
     5761 gactggaacg caaccacatt ggtgaaatcg ggccccgcgc attccagcat gctgccgggt
     5821 gcttgcctgg tgtaaatctc cgaatagcat tcgtcgctca gaatcatgaa gccgaaacga
     5881 tcggctaatc gcttcaggcg cgcgaaatag ccgcgcgagg ccaccgcgcc ttgcggattt
     5941 gccggcgagg cgatgaagat cgcgacggtt ctcgccagcg tggcatcatc gagcgcatcg
     6001 aggtcgggga gaaatccgtt ggcgagcgtg gtcggcagat aaatcgcctc gcagcccgcc
     6061 gtgcgcgcgc cggcggcgta agcgggataa aacgggttcg gtatcaggat cgcaggtctg
     6121 cccttgcgtt cgccggcata gcgggcggcg gtgagcgcgg cgaaaaacag gccctcgcgg
     6181 ctgccgttga gcaccagaag ctccgtctct ggatctaccc cgcgcggcag gttgaaccgg
     6241 gtggaaagcc agttcgcagc cgcccgccgg aacggctcga tgcccttggc gatgggatag
     6301 cggccgaaat cgccgatgtg gttggcgagc accgggccga caaaggccgg aaccgggtgc
     6361 tgcggctcgc ccaacgatag cgtaatcaat ggcttggctg gttgatacgg cgccaaaagc
     6421 tcagtcagcc ttgcgaatgg cgaccgttcg ctttcctgat tgccggagcc ctgcggcgcg
     6481 cgggatgaag cggtcattgc catacgtgaa gcgccagcac tgtttggggc ggccggtcac
     6541 ccgaagaccg gcctgaaacg gatcaaatca ccatagatac ggcgaggtta agacacgatt
     6601 aaccatcgat caaacgccgg gttccatgct tgatctaggg caccgatcca acggaagcgc
     6661 tctgcgagcg tacttcaata ccgcatcccg tggccatcgg ccagaccggt gcgttttgcc
     6721 gaatttgatg aggcatttca tgcggcgggc aactccatac ccctatacca tgccctttgg
     6781 gcacggcgtg gacaaaacat ttagatcggg attcgcgggc cacgaaatgg gccgggatcg
     6841 tcgaatgtac cgactttcag aatcactgcg ggcaaattcg gccgccacgt ggctggatgc
     6901 gcgccgcatt tggctattag cgttcgacaa ctgttccaac cttgccgtcg ggcgatcatg
     6961 aaggttctag ttacgggaag tgccggtcac cttggcgagg ccttggtccg cacgttgaca
     7021 aacatgcaat acgaggtcgt cggcctcgat atccttgagt ctccatttac cacgcgcgtg
     7081 ggctcgataa ccgaccgctc ttgcgtgagg cattgcatgc atggcgtgca ggcggtcttt
     7141 catgcagcca ccctgcacaa gccgcacatc gctacccatc caaggcaaga cttcgtcgac
     7201 accaacgtta ccggtacgct caatctgttg gaggaagcgg cagcggcagg cgtagtgtct
     7261 tttgtctaca caagcacgac cagcatcttc ggcgatgccc ttgtgccgcc acctggagcc
     7321 cctgccacgt gggtgaccga ggaggtcgcg ccggtgccga agaacatcta tggcgtgacc
     7381 aaggccgcag cggaggatct ctgccagctg ttccatcgaa accaggcact cccctgcatc
     7441 gtgctcagaa cgtcgcgctt tttccccgag gaagacgaca acaaggatgt gcgggaggcc
     7501 tactcagacg ataacgtcaa ggtgaatgaa tatctctacc ggcgcgtcga gatcgaagat
     7561 gtcgtgagcg cgcatttgtt ggcctctgag cgagcgccgg cgatcggctt tcgcaaatac
     7621 atcgtcagcg cgaccacgcc gttcctgcct gacgacctgc cggccctgcg ggtcgacgcc
     7681 cccagggtcg tgcggctgcg tgttccggaa tatgaagccg agtacgaacg ccgcggctgg
     7741 aaaatgtttc caagcatcgg acgggtttat gtcaacgacc gcgcgcaacg tgaacttggc
     7801 tggcagccgc ggtattgctt caacgacatc atcggccgcc tgaaagcagg ggacgatctg
     7861 agaagtcctc tggcgcgggt gacaggatca aaaggatacc atgccgagcc gtttcccgac
     7921 ggaccttatc cggtcgaata gattttgttg ggggcaccac cgacgcgatc ctgcccacgc
     7981 gtcatccact caagttcgac cgaaatcagc gcactcggcg cgctcaattg ccaaaaagaa
     8041 aggggcccca acttgcgctg gagcccctca acggtccggg cattgccggg tatgtgccca
     8101 aaccgtagtt ctgggcgcga cctcgtgaga tcgcgccccg ggagagaact tacatgttgt
     8161 aagcgcgctc ggt
//
LOCUS       DATNOI010000478         3187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_582268,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000478 DATNOI010000000
VERSION     DATNOI010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582268"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(350..532)
                     /locus_tag="VN968_19085"
     CDS             complement(350..532)
                     /locus_tag="VN968_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31375.1"
                     /translation="MMKIVIIAGATLAVVTAAGAADIPRPQPVYQTAQLGKMPIGKTP
                     VGKTPIGKPPVARRAY"
     gene            complement(793..1965)
                     /locus_tag="VN968_19090"
     CDS             complement(793..1965)
                     /locus_tag="VN968_19090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HXL31376.1"
                     /translation="MIVRQFISWIRTAPAGERAEATRALARAWLISDLSAEDRAAAEG
                     ALLMQLDDASPLVRQAMAEVFARSTEAPAAIVQALSLDQPSVALPVLEYSPLLIDADL
                     VDIIATGNCEMQCAIARRNNLPASVCAAIAEVGTAAAALELIENAYAELAPFSWDRIV
                     ERHGHLAAIRESMLVLEDLPAATRVALVAKLSDTLAQFAVARNWLSADRAARVASEAR
                     ERSTVNIAAGSNGDDVRGLVRHLRASGQLTAGLILRALLSGNQELFNAALAELADLPP
                     ARVAALLHDCGSASLQALLIRAGLPESTFAAFRVALEVNHETGFVDTAGGAARLQRRM
                     VERVLTHCATDRKAAEPLLILLRRFATESAREEARMFCDELMAEEATAPLQHDLAA"
     gene            complement(2368..>3187)
                     /locus_tag="VN968_19095"
     CDS             complement(2368..>3187)
                     /locus_tag="VN968_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXL31377.1"
                     /translation="QLGYGKYADAITKSSSGNYSVSDPAARDAIMKLRDDPDAASSMA
                     AVLTQSNSFKLTGDIGRRPTDAELYMAHFMGVGGAAKLISNAEDHPNASGARLFPNAA
                     AANRSIFYDRGGRERSVAQVYSVLTQRYDSAANSSATRSAMASAGVTPASGTFASAAA
                     GAAPIDNAAYLSSFPDSRAATASAAASQPALKTASLQPSEPIFRSLFQAGERSQPVSP
                     AVHELWGSSTSLTSVAPTSVTITSPISSLPTRTPNVRAPQPLDLFSDRSGAFSS"
BASE COUNT          601 a          993 c         1026 g          567 t
ORIGIN      
        1 ggctctgaaa tcaacgtagt acgggccctt tgcggatccc gaccccggct ttcgaacaga
       61 ctgtggcgcc ggtttcgatt caatgggcgc ctcaccggaa acaagcgcat tcgcgttggc
      121 ggtgcgcttg tggacgtccc gtctctggga agtgaaacgc tccgaaccgg cgcgaacgtc
      181 agtctcgtgt gcagaagccg attggatttc gcattggagc aaaacagcga gcgtgaatcc
      241 cgcaagcaag catagtcctg atctcgtcgg cctgtttgcc acgcttcgcc cctcacctgt
      301 cgacgacaaa ggcccgattc aaccagacct tttgagtcca taccgcgtat cagtaggctc
      361 gcctcgcgac gggcggcttc ccgatcgggg tcttcccgac tggagtcttg ccaatcggca
      421 tcttgccgag ttgggccgtc tgatagaccg gctgtgggcg cgggatgtcg gcagcgccag
      481 cggcagtcac cacggcaagc gttgcacccg ctataatcac aattttcatc acggtacttt
      541 tcctccaggt tagaagaaac aaggcagtca cctgccgagc gttcccctca gacacgccgt
      601 gagcatgaac gccggaatac gtcaccagtg cggcagacag gacaagttcg agggaaaggt
      661 ctgcagtgcg gcagatatga gacatgcggg aacctcctat tgggtcaatg gagcgatagc
      721 cgaacgccgt gattcaagct tccaaaccgg agcttcgaat catgcgcggg aacgacgtcg
      781 agggtgatgc gatcaggccg cgagatcgtg ctgaagcggt gctgtggctt cttcggccat
      841 cagttcgtcg cagaacatgc gggcttcttc ccgtgccgac tcggtcgcaa agcgccgcag
      901 caggatcaat agcggctcgg cggccttccg atcggtggcg caatgcgtca gcacgcgttc
      961 gaccatccgg cgctgcaatc tggcagcacc tccggccgtg tcgacaaatc cggtttcatg
     1021 attgacctcg agcgccaccc ggaacgccgc gaaggtcgat tccggcaatc cagcccgtat
     1081 cagcagcgcc tgcaggctgg cgctgccgca gtcatgcagc agtgcggcga cgcgggccgg
     1141 cggcagatcg gccaattcgg ccaacgcggc attgaacagc tcctggttgc ccgacaacag
     1201 cgcgcgcagg atcagaccgg cggtgagctg gccgctagcg cgcagatgcc gcaccaggcc
     1261 acgcacgtcg tcgccgttcg agcccgccgc gatattcacg gtggagcgct cgcgcgcctc
     1321 gctcgcgacc cgcgccgccc gatcggcgct cagccaattg cgggcgaccg cgaactgcgc
     1381 tagcgtatcc gacagtttgg ccaccagcgc cacccgcgtc gctgcgggga gatcttccag
     1441 caccagcatc gattccctga tcgcggcgag atggccgtgg cgctcgacga tgcgatccca
     1501 ggaaaacggc gccagttccg cgtaggcatt ttcgatcagt tcgagcgctg ccgcggcggt
     1561 gccgacttcg gcgatcgccg cacaaaccga ggccggcagg ttgttgcggc gggcgatggc
     1621 gcattgcatc tcgcaattgc cggtggcgat gatgtcgaca aggtctgcat cgatcagcag
     1681 cggtgaatat tcgagcaccg gaagcgccac cgacggctgg tcgagcgaca gcgcctgcac
     1741 gatggcggcg ggcgcctcgg tgctgcgcgc gaacacctcg gccatggcct ggcgcaccag
     1801 cggcgacgca tcgtcgagct gcatcaagag cgcgccttcg gcggcggcgc ggtcctcggc
     1861 ggataaatcc gagatcaacc aggcccgcgc caaagcccgc gtggcctccg cccgctcgcc
     1921 cgccggagcg gttcttatcc aactgatgaa ctgccgaacg atcattgtcc cagccagccg
     1981 acgcaacctg taaacgacac cgagatgcca ctgtcataca aaataaacca cgacgcttaa
     2041 caaagcgttc accataagca gctggtttta ttgacatttt gttaagggtg cggaggtggg
     2101 tgggtaagtg caaatttctc cgcgtgcgaa ggattcgttt tttcgtcatg gccgggcttg
     2161 tcccggccat ccacgtctta acagcctagc aacaagaaag acgtggatgc ccgggacaag
     2221 cccgggcatg atgagtttcg cgaccggatt gcttcgcttc gcacgcactg cctcatcctg
     2281 aggagcatcg cgcagcgatg cgtctcgaag gatggccgcg aactcatggt tcgagacggc
     2341 gcaagcgcgc ctcctcacca tgaggggtta actgctgaag gcgccgctgc ggtcgctgaa
     2401 gaggtcgagc ggttgcggcg cgcggacatt tggtgtgcgc gttggcagcg aggagatcgg
     2461 agatgttatc gtgaccgatg tcggcgcgac cgaggtcagc gacgtcgagc tgccccacaa
     2521 ttcatgaacc gccggcgaga ccggctggga tcgttcgccg gcttgaaaca gcgagcggaa
     2581 gatcggctcg cttggctgca gcgatgcggt cttgagcgcc ggctgcgacg ccgcagccga
     2641 ggccgtcgct gcgcgcgaat ccggaaaact cgagagatag gcggcgttgt cgatgggtgc
     2701 tgcgcccgcc gccgcgctag cgaacgtccc actcgccggc gtcacaccgg ccgacgccat
     2761 cgcggaccgc gtcgccgatg aattggcggc gctgtcgtag cgctgtgtca gcaccgaata
     2821 gacttgcgcg acgctgcgct cccgtccgcc gcggtcgtag aagatcgaac ggttcgcagc
     2881 ggcagcgttc ggaaacagcc gcgcaccgga cgcattcgga tggtcctcgg cattcgagat
     2941 cagcttggcc gcgccgccga cacccatgaa atgcgccata tagagttcgg cgtcggtcgg
     3001 gcggcggccg atgtcgccgg tgagcttgaa gctgttggat tgggtgagaa ccgccgccat
     3061 cgacgaggcg gcatcgggat cgtcgcgcaa tttcatgatc gcgtcgcgcg cggcgggatc
     3121 gctgaccgag taattgccgg acgaagattt tgtgatggcg tcggcgtatt tgccgtagcc
     3181 gagctgg
//
LOCUS       DATNOI010000479        11843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_24743,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000479 DATNOI010000000
VERSION     DATNOI010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11843
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24743"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /gene="paaI"
                     /locus_tag="VN968_19100"
     CDS             complement(<1..433)
                     /gene="paaI"
                     /locus_tag="VN968_19100"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HXL31378.1"
                     /translation="MNVKATLSPDDLARACADAMWQDDDASKGLGMEIRRIKPGEATL
                     TMTVQPHMVNGQGIAHGGFIFTLADSDFAFACNSHNERAVAAQCNISFIRPGKLGDLL
                     IATAREISRTGRSGIYDVRVTVDDTPIAELRGHSRTVGGAWV"
     gene            669..1691
                     /locus_tag="VN968_19105"
     CDS             669..1691
                     /locus_tag="VN968_19105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HXL31379.1"
                     /translation="MIPAPKEIDGPPVTVAARCVRLLTKADDPASLAPVVEARRLSRA
                     ANELLIEVKAAAVNPSDVKAATGLMAYAVFPRTPGRDYAGIVIDGPADWIGREVFGSS
                     GDLGIRRDGTHATHLAVEADAVVEKPKSLSWEEAAGIGVPFVTAMEGFRRAGMPAKDE
                     TVLVMGVNGKVGQSAVQIASWNGARVIGVVRKREPYEGHTNSEVEVIDASATDVATRV
                     RELTDGKGADIVFNTVGDPYFQAAHQSLAVRGRQILIAAINPIVPFNILEFYRGQHSY
                     VGIDTLGLSSPATGAVLKELVAGFASGHLKPFPIKSSAIYPLEEAKAAFVAVAGSSRD
                     RVILRP"
     gene            1861..2958
                     /locus_tag="VN968_19110"
     CDS             1861..2958
                     /locus_tag="VN968_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HXL31380.1"
                     /translation="MDSTHIVVFAGLLIGLVYGTVGLLSGFCLMSSLRGWWAEGDRRL
                     IRSLALALGVAIAATQLLAAGGLVDLGKTIYLQPSFSAPLMFFGGLLFGYGMVLSNGC
                     GSRALVLLGRGNLRSFVVVIVLGISAQMTLKGLVAPARIVLLQASQTTATVTSVPALL
                     STFGLNEISARMLGASAISGALIIFAFAHAPFQRALGQIAAGLVVGLLVAAGWFATGY
                     LGADDFNPTPVTSLTFVAPIADTLQYVMLSTGLTLNFGIATVAGVFAGSCVTALATRR
                     FHWEGFSSPRHMLRSAGGAALMGAGGAMAYGCSIGQGLTGLSTLALASFVAVAGILLG
                     TAAGLRGVLRVRPLVSAQDARVSAQDARSGP"
     gene            complement(2988..4211)
                     /locus_tag="VN968_19115"
     CDS             complement(2988..4211)
                     /locus_tag="VN968_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/OsmC family
                     protein"
                     /protein_id="HXL31381.1"
                     /translation="MPTERFQFTGSEGHKLAASLDLPEREPEAYALFAHCFTCGKDVL
                     AAKRIAVALAAKGIAVLRFDFTGLGSSEGDFANSTFSSNVADLVRAADHLRETRKAPT
                     ILIGHSLGGAAILAAAGQIPDARAVVTIAAPSDPAHVTGLFKDRIDDIRKHGKVEVSL
                     AGRPFHISSEFLDDIAHHGLMAHVAKLHKALLIMHAPTDDTVGIDNATDIFLAAKHPK
                     SFVSLADADHLLTGKRDASYVADVIAAWAERYLDPVISEPAVDRGAAPRSVVVRETRN
                     SKFQQTVSIGPHHLLADEPVAAGGEDTGPGPYDFVLAGLGACTSMTMRLYAERKSLPL
                     ERVTVTLNHSKIHAEDCAECETKAGMLDQFDRVITMEGALDAEQRKKLMEIADKCPVH
                     RTLTSEIHIVTRAAD"
     gene            complement(4283..4948)
                     /locus_tag="VN968_19120"
     CDS             complement(4283..4948)
                     /locus_tag="VN968_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HXL31382.1"
                     /translation="MADNSDEPPTSKLTRSKQRWAREGRFLTGKTSRPEQQRLPPGQH
                     LTKDWPTLDLGLTPNISRQRWRLDVYGAVEHPIFWDYAQFTAQPQTKFLSDIHCVTTW
                     SRYDNQWEGLATRDLLMACQPRDEARHVVLHSHDGYTTNLTLEDFAAEDALLAHAWSG
                     APLEQEHGGPVRLVVPHLYFWKSAKWLQSIEFLAEDKPGYWEVRGYHNRGDPWKEQRY
                     SAG"
     gene            complement(4962..6282)
                     /locus_tag="VN968_19125"
                     /pseudo
     CDS             complement(4962..6282)
                     /locus_tag="VN968_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(6324..7544)
                     /locus_tag="VN968_19130"
     CDS             complement(6324..7544)
                     /locus_tag="VN968_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXL31383.1"
                     /translation="MSDPAPVMSEHPPVTDWVHDFDHTDPRWTENPFPIWDELRAECP
                     VVHTKRFLGCYLPTTYEAVKQIAYDTEHFSSRRVIVRDVRPEATASAPPITSDPPEHK
                     PAKQFLLPPFTPDAMKKLEPRVRAICNELIDEFINDGKCDAAARYTKHIPVRAIAHML
                     GIPEKDSDHFIKWIHEILELGIKNDDVLMRAVQEMTEYFAGHIEQRKQQPTDDLISTL
                     MNAKDKNGQPLSDTHVLGSLRLLLIAGIDTTWSAIGSSLWHLAKTPADRARLIAEPEL
                     MPTAVEELLRAYSPVTMAREVMKETVVSGCPVKPGNMVLLSFPAANRDPTMFPDADKV
                     VIDRKENRHAAFGLGIHRCVGSNLARMEMTVAIEEWLKRIPDFGLDPAGQVTWSEGTV
                     RGPRQLPMLFGKAG"
     gene            complement(7612..7827)
                     /locus_tag="VN968_19135"
     CDS             complement(7612..7827)
                     /locus_tag="VN968_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HXL31384.1"
                     /translation="MSKSPKLRLHVDQDKCQGHARCKSLAPELFELDEFGNAHEVGDG
                     TVPAGLEDKAWLAQTNCPETAIEVTEE"
     gene            7993..8679
                     /locus_tag="VN968_19140"
     CDS             7993..8679
                     /locus_tag="VN968_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL31385.1"
                     /translation="MAADWHSTDWLTVRDLPGILPTQQRRSQETTVALLEAAAAMLRE
                     RSLDELSIEDLCRRVGVTIGAFYGRFESKDAFFSALMSLAARKALAAVRAAVADQDNP
                     GTGLQEACRRVVEVAVDVVRRNVGVVRAAAQYESIYPERWGTIRATGSGMVDLATPLL
                     LARMGRGRIPAKTRSIGFAFQMMFGTLCNAVLHKPKLVSLDEPEMVDRLALTMFLQLK
                     HEAGAEKPVK"
     gene            complement(8704..10140)
                     /locus_tag="VN968_19145"
     CDS             complement(8704..10140)
                     /locus_tag="VN968_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXL31386.1"
                     /translation="MAFDQGLVRATACAIVDKLNKSEVTPLDLLDALEQRIAGVDGQV
                     NALPTLCFDRARTHAEALMKKPLGERGLLAGMPVPIKDLTNVAGVKTTQGSPIYKDTI
                     ATRSDVVVERLESNGAVIYAKSNTPEFGAGANTFNEVFGATLNPWDLSRSAAGSSGGA
                     AAALATGMAWLAHGSDMGGSLRNPASFCGVVGLRPSIGRVAHTPTFKIDRNLTVHGPM
                     ARNVEDLALLLDAMSGEHPADPLSLPALPVSFLSAARSGNKPKRVAYSPDLGITPVDP
                     EVVAITRKAAARFAEAGAIVEEAHPDLREAHECFQVLRAFDFAISKAALLRTKRDLLK
                     PEVIWNIEQGLKLTVEQLERAEGQRVAMAARTLEFFRTYDLLLAPATIVAPFPIEHRY
                     VAECAGQKFDNYVEWLAIVYAITLVCCPALSLPCGFTASGLPVGLQMVAAPRGEAQLL
                     AGARLLEDILGVSGTTPIDPRGKPSPAK"
     gene            10255..11091
                     /locus_tag="VN968_19150"
     CDS             10255..11091
                     /locus_tag="VN968_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL31387.1"
                     /translation="MSTPFDAPDWRDMSQQDRDLGLNNGVAVAGSGDMVAGWERRSAQ
                     MRARDSDHLDLRYGPRERNRIDFFKAAEKAPTVLFIHGGYWQMRAKEVFAIFAAGPLA
                     HGINVATIGYTLAPDATLDEIVAEIHQGLDYLAEELPALGGDANRIVVSGWSAGGHLT
                     AMALSHRCVKAAMAISGIYDLEPIRHSYLNVKLGLDEATSRRNSPVMQPGGAMKPLAL
                     VAGAAELPLLRRQTADFACHRAKYGLPVIYEEIPGADHFTIMHEMMSPTGRITALIRE
                     LV"
     gene            complement(11183..>11843)
                     /locus_tag="VN968_19155"
     CDS             complement(11183..>11843)
                     /locus_tag="VN968_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL31388.1"
                     /translation="SGDQLKALADRLRSAHQIDATTQIVDLRNPADLARLAKDAADID
                     ILVNNAGDIPGGSVDKIDEATWRHAWDLKVFGYVNLTRAIYAAMKARGHGVIVNDIGA
                     AGERFDANYICGSAGNAAIMAFTRALGGKSLADNIRVVGINPGPVGTDRHITLLKTRA
                     KNQFGDESRYTEFQKGLPLGRPAHAREIGDLMAFLASDRSGYTSGVIYTVDGGLSAGW
                     G"
BASE COUNT         2068 a         3569 c         3960 g         2246 t
ORIGIN      
        1 gcacccaggc gccgccgacg gtgcgggaat ggccgcgcaa ctcggcaatc ggggtgtcgt
       61 cgacggtgac gcgcacatcg taaattccgg aacgcccggt gcgcgagatt tcccgcgcgg
      121 tcgcgatcag gaggtcgccg agcttgcccg gccggatgaa ggagatgttg cactgcgccg
      181 cgacggcgcg ctcgttatgg gagttgcagg caaaggcgaa gtccgaatcc gccagcgtga
      241 agatgaagcc gccatgggcg atgccctggc cgttgaccat gtgcggctgg accgtcatcg
      301 tcagcgtggc ttcgccgggc ttgatccgcc ggatctccat gccgagaccc ttgctggcgt
      361 cgtcatcctg ccacatcgcg tcggcgcagg cgcgcgcgag atcgtcgggc gaaagtgtgg
      421 ccttgacgtt caccgtggtc tccccgcgca tttccgcggc cgcctgacga tcaggaccgg
      481 atccaagacg ttctccccaa gacgttctct gaccaagatg ttctctgagt tcgcagtcgg
      541 gcttgttgga acaagcctct tgaactcagc gatcagtccg ttagtcatga tgcgaagcga
      601 ccatggtgtc aacgaggccg tcaggcatca aatcgcgttc gaaagccgcc aagaaagcgt
      661 gaacatccgt gataccagcc cccaaagaaa tcgatggccc gccggttacc gtcgcggcgc
      721 gctgcgtgcg tctgctgacg aaggccgacg atccggcgtc gctcgcaccc gtggtcgagg
      781 cgcgccggct atcgcgggcc gcgaacgagc tgttgatcga ggtcaaggcg gcggcggtta
      841 atccgtccga cgtcaaggcc gccaccggcc tgatggctta cgcggtattt ccgcgcaccc
      901 ccggccgcga ctatgcgggg atcgtgatcg acggcccggc cgattggatc gggcgcgagg
      961 tgttcggctc gtcgggcgac ctcgggatcc ggcgcgacgg cacccatgcc acgcatctcg
     1021 cggtcgaagc cgacgccgtg gtggagaaac cgaaaagcct gtcgtgggag gaagccgccg
     1081 gtatcggcgt tcccttcgtc accgcgatgg aaggtttcag gcgcgccgga atgcccgcga
     1141 aagacgagac ggtgctggtg atgggcgtca acggcaaggt cggtcaatca gcggtgcaga
     1201 tcgcgagttg gaacggcgcg cgcgtgatcg gcgtggtgcg caagcgcgag ccttatgagg
     1261 gccacaccaa ttcagaagtc gaagtgatcg acgcatcggc aaccgacgtc gccacgcggg
     1321 ttcgcgaact caccgacggc aagggcgccg acatcgtgtt caacaccgtc ggcgatccct
     1381 attttcaggc cgcgcaccaa tcgctggccg tgcggggacg tcagatcctg atcgccgcga
     1441 tcaatccgat cgtgccgttc aacatcctgg aattctatcg cggccagcac agttatgtcg
     1501 gcatcgacac cctggggctg tcgtcacccg cgaccggcgc ggttctgaag gaactggttg
     1561 ccggttttgc gagtggacat ctcaagccgt tcccgatcaa gtcgagtgcg atctatccgc
     1621 tggaggaagc aaaagctgcc tttgtcgcgg tcgccggctc atcgcgcgat cgcgtcattc
     1681 ttcggccgta acgggtaaga caatgaattc aggccgtcat tgcgaaccga cgggtcgcgc
     1741 gaatgcgcgc ccgatgataa acttcgcgaa gcaatccaga actcgggcaa agtgcttaag
     1801 tgcaggattg cttcgtccgc tgcgctcctc gcacggctga gactccgagg aaccgaaccc
     1861 atggattcca cccacatcgt ggtctttgcc ggcctgctga tcggcctcgt ctacggcacg
     1921 gtcggattgc tgagcgggtt ttgcctgatg agcagcctgc gcggctggtg ggcggagggc
     1981 gaccgccgcc tgatccggag tcttgcgctg gcgcttggcg tggcgatcgc ggcaacgcag
     2041 ctgttggcgg caggtggtct cgtcgatctc ggcaagacga tctatctgca gccgtcgttt
     2101 tcggcgccgc tgatgttctt cggcggcctg ctgttcggtt acggcatggt gttgtcgaac
     2161 ggctgcggct cgcgtgcgct ggtgctgctc ggacgcggca atctgcgttc gttcgtggtg
     2221 gtgatcgtgc tcgggatatc ggcgcagatg acgctgaagg gcctggtcgc gccggcgcgg
     2281 atcgtgctgc tgcaggcgtc gcagacgacg gcgacggtga cgtcggtgcc ggcgctgctg
     2341 tcgacgttcg gcctcaatga gatatcggcg cggatgctcg gggcctccgc gatcagcggt
     2401 gccctgatca tctttgcgtt cgcgcacgcg ccgttccagc gcgctctcgg gcagatcgcc
     2461 gcggggctcg tcgtcggcct gctggtcgcg gccgggtggt tcgccaccgg ttatctcggc
     2521 gccgacgact ttaatccgac gccggtgacg tcgctcacct tcgtcgcgcc gatcgccgac
     2581 acgctgcaat atgtgatgct gtcgaccgga ctgaccctga acttcggcat tgcgaccgtt
     2641 gccggcgtat tcgccggaag ttgcgtgacg gcactggcga cgcgccgctt tcactgggag
     2701 ggtttttcgt cgccacgcca catgctgcgc tcggcaggcg gcgcggcgct gatgggcgcg
     2761 ggcggcgcca tggcctatgg ctgctcgatc gggcaggggc tgacaggtct ctcgacgctg
     2821 gcgctggcgt cattcgtagc ggtagccgga attttgttag gcacggctgc gggactgcgg
     2881 ggcgtgctgc gggttcggcc gctggtgtcg gcgcaggatg cgcgggtctc ggcgcaggac
     2941 gcgcggtccg gcccgtgagg tgaggtcgcg ggccgctcgc ccgttcgtca atccgccgcg
     3001 cgcgtcacga tatggatctc cgaggtcagg gtgcgatgca ccgggcactt gtcggcgatt
     3061 tccatcaatt tcttgcgctg ctcggcgtcc agtgcgccct ccatggtgat gacccggtcg
     3121 aactggtcga gcatcccggc cttggtttcg cattcggcgc aatcttccgc gtggatcttg
     3181 ctgtggttca gcgtcaccgt cacgcgttcc agcggcagcg atttgcgctc ggcatagagg
     3241 cgcatggtca tcgaggtgca ggcgccgagg ccggccagca cgaaatcata cggtccgggt
     3301 ccggtgtcct cgccgccggc ggcaaccggc tcatcggcca gcaggtgatg cggcccgatc
     3361 gaaaccgtct gctggaattt gctgttgcgg gtctcgcgga ccacaacgct gcgcggcgcc
     3421 gcaccgcgat cgacggctgg ctccgatatg acgggatcga ggtagcgctc ggcccaggcg
     3481 gcgatgacat cggcgacata ggaagcatcg cgcttgccgg tcaggagatg atcggcgtcc
     3541 gccagcgaca cgaaactctt gggatgcttg gcggcgagga agatatcggt ggcgttgtcg
     3601 atgccgacgg tgtcgtcggt cggcgcgtgc atgatcagca acgccttgtg cagctttgcg
     3661 acatgcgcca tcaggccgtg atgggcgatg tcgtcgagga attcgcttga gatatggaac
     3721 ggacggccgg cgagcgagac ttcgaccttg ccgtgcttgc ggatgtcgtc gatgcgatcc
     3781 ttgaacaggc cggtgacgtg ggcgggatcg gagggggcgg cgatggtgac gacggccctg
     3841 gcatccggaa tctgcccggc ggcggccagg atggcggcgc cgccgagact gtggccgatc
     3901 aggatcgttg gcgccttgcg cgtttcgcgc agatggtcgg cggcgcgcac cagatcggcg
     3961 acgttcgacg agaacgtaga attggcaaag tcgccttcgc tggagccgag gcccgtaaaa
     4021 tcgaaccgca gcacggcgat gccttttgcc gccagcgcca ccgcgatgcg cttggccgcc
     4081 agcacatcct tgccgcacgt aaaacaatgc gcgaacagcg cataggcctc cggctcccgc
     4141 tcgggcaggt cgagcgaagc cgcgagtttg tggccctcgg agcccgtgaa ttggaaacgt
     4201 tcagtcggca tggttggcct ccatgggtga tgctcttcac ctcacccata gggagagatg
     4261 acgatgaact cttgcatcga actcagccgg cggaatagcg ctgttccttc caagggtcac
     4321 ctcgattgtg atagccgcgg acttcccaat agccgggctt atcctcggcg aggaattcga
     4381 tgctctgcag ccatttcgcg cttttccaga aatacagatg cggcaccacc agccgcaccg
     4441 ggccgccatg ttcctgctcc agcggcgcgc ccgaccaggc gtgcgcgagc agggcgtctt
     4501 cggcggcgaa atcttccagc gtgagattgg tggtgtagcc gtcatgggaa tgcagcacca
     4561 cgtggcgcgc ttcgtcgcgc ggttggcagg ccatcagcag atcgcgggtc gcgagcccct
     4621 cccactggtt gtcgtagcgc gaccatgttg tcacgcagtg gatgtcggat aagaatttcg
     4681 tctgcggctg cgccgtgaac tgagcgtaat cccaaaaaat cggatgctcg accgcgccgt
     4741 agacgtcgag gcgccagcgc tggcgggaaa tgttcggcgt caggccgaga tcaagggtgg
     4801 gccagtcttt ggtcagatgc tgtcccggcg gcaggcgttg ctgttccggc cgcgaggttt
     4861 ttccggtcag gaagcggcct tcccgggccc agcgctgttt gctgcgcgtc agctttgaag
     4921 tcggcggttc gtcgctgttg tcggccatat ctcaagcatc tttattcgtg acggtgcatc
     4981 gccgattcgt gcttggtgtc gcgcatcgtc gcgtagatga tcagcgacag acatatcatg
     5041 ccgctgagat aataatagaa ccagtcttcg tgttcgagcg acttgaaata gagcgcgacc
     5101 gcgggcgcgg tgccgccaaa tacggatacc gtgatcgcgt agggcaggcc gacaccgagc
     5161 gcgcacattg gtcgggaaca attcggcctt cacgatggcg ttgatcgagg tgtaaccggc
     5221 gacgaagatc caggccgcgc aaatcagcag gaaggcggtg aagggtgact tggtctcctt
     5281 cagcgtggtc aagatcggga tcgtcgccag cgtgccggcg acgccgaaaa agatcagcag
     5341 cggtttgcgg ccgatgcggt cgctcaatgc gccgtagagc ggctgcagca aggtcgcaaa
     5401 gatcagcgaa ccgaagatga caaaggtggt ctgatcttcg gtgaggccga ccgacaattt
     5461 cacgaaagtc tgcatatagg tcgtgaaggt atagaacgcc gcggtgccgc cggcggtgag
     5521 gccgaccact agcaatagct cgcgcggata tttcaggagg ccgcggatcg aaccggtcgg
     5581 ttttgcaagc tttttggctt cgacgaacgc ctcggtctca tgcagattgc gccgcatcac
     5641 cgcggcgaaa acggcaagcc ccgcgccgat cacgaacgga atccgccagc cccaggcctt
     5701 caattcctcc ggcgtcaaga acaccttttg cagcagcaac agcacgatga tcgcggtgag
     5761 ctggccgccg atcagggtga cgtattgaaa gctggaatag aagccgcgat ggttggcatc
     5821 ggcgacctcg ctcagatagg tggcgctggc gccgtattcg ccgccgaggc tgagaccctc
     5881 gataattcgc gcggccgcga gaatggccgg cgcggcgagg ccgatcgagg cataggtcgg
     5941 cgtcaccgcg atgatcaacg agccgaagca catgcagacc accgacagcg tcagcgacag
     6001 ccgcctgccg tagcggtcgg cgatgaaacc gaacagccag ccgccgagcg gccgcatcag
     6061 gaaagtcgcg gcaaacagca cggccgcgtt gagctgctgc accaccgggt cgctgttcgg
     6121 aaagaacgcc ggcgcgaagt agagcgcgaa cgccgtgtag gcgtagaaat cataccattc
     6181 gacgagattg ccgaccgacc cgatgaaaat cgccttgatc cggcgttcga cgtcggcgag
     6241 atcgagatga tcggccaccg gcttcacggc ttcaacagtc atgtcggcct ccagcaacaa
     6301 tgaaggccgc ttgttagcag acttcagccc gcctttccaa acagcatcgg aagctggcgc
     6361 ggtccgcgca cggtgccttc cgaccaggtg acctgaccgg ccgggtccag cccgaaatcg
     6421 ggaatccgct tcagccattc ctcgatggcg accgtcattt ccatgcgcgc gaggttggag
     6481 ccgacgcagc ggtgaatgcc gaggccgaag gcggcgtggc gattctcctt gcggtcgatc
     6541 acgaccttgt cggcgtcggg gaacatggtg ggatcgcggt tcgcggcggg aaacgacagc
     6601 agcaccatgt tgccgggttt tacggggcag ccgctgacca cggtttcctt catcacctcg
     6661 cgggccatcg tgaccggcga gtaggcccgc agcagctcct cgaccgcggt cggcatcagt
     6721 tccggttccg cgatcagacg cgcgcgatcc gcaggcgttt tcgccagatg ccacagcgag
     6781 gagccgatcg cgctccaggt ggtgtcgatg ccggcgatca gcaatagccg cagcgatccc
     6841 agcacatgcg tgtcggacaa gggctgaccg ttcttatcct tggcgttcat cagcgtcgaa
     6901 atcagatcgt cagtcggctg ctgcttgcgc tgttcgatat ggcccgcgaa atattcggtc
     6961 atctcctgga ccgcgcgcat caggacgtca tcgttcttga tgccgagttc cagaatttcg
     7021 tggatccatt tgatgaaatg gtcgctgtcc ttttccggga tgccgagcat gtgggcgatc
     7081 gcgcgaaccg gaatgtgctt ggtgtagcgc gccgcggcgt cgcatttgcc gtcattgata
     7141 aattcgtcga tcagctcatt gcagatggcg cgaacccgcg gctccagctt tttcatggca
     7201 tcaggtgtga acggcggcag caggaactgc ttggcgggct tgtgttccgg cgggtcggat
     7261 gtgatcggcg gggccgatgc ggtcgcttcc ggccggacgt cgcgcacgat gacgcgtcgc
     7321 gaggaaaaat gttcggtgtc gtaggcgatc tgcttgaccg cttcataggt agtcggcagg
     7381 tagcagccga gaaagcgctt ggtgtgcacc accgggcatt ccgcccgcaa ctcgtcccag
     7441 atcggaaacg ggttttcggt ccagcgcggg tcggtatggt cgaaatcgtg aacccaatcg
     7501 gtgacgggcg gatgttcgga cattacgggg gcgggatcgg acatcggggc gaatcctctg
     7561 gtttattcgc ttgagtttgt tgcagaaatc gtcggctggg cgggaaacgg actattcctc
     7621 ggtcacctcg atcgcggttt ccggacaatt ggtttgtgcc agccacgcct tgtcctcaag
     7681 ccccgcgggc acggtgccgt cgccgacctc gtgggcgttg ccgaattcat cgagctcaaa
     7741 cagttctggc gccagcgatt tgcagcgggc gtgtccctga cacttgtcct gatcgacgtg
     7801 cagcctcaat ttgggggatt tcgacattcg accagccctc ccttggtatc gccgcttgcg
     7861 agcgctgcgt tgtcgcggcg gcaatcgctg gcggcttcgc gacttcgacc tcacaggatg
     7921 cgcgttgttt ttgttgttgt gaattatagg catgactata atataaccgt caatacaaat
     7981 ttggatgatc gcgtggcggc agattggcat tcgacagact ggcttaccgt tcgggatctc
     8041 cccggcattt tgccgacgca gcagcgccgc agccaggaaa ccacggtcgc gctgctggaa
     8101 gccgccgccg cgatgttgcg cgagcgctcg ctcgacgagc tctcgatcga ggatttgtgc
     8161 aggcgcgtcg gcgtaacgat cggcgccttc tacggccggt tcgaaagcaa ggatgccttc
     8221 tttagcgcgc tgatgtcgct ggcggcacgg aaggcgctgg ccgccgtccg cgccgcggta
     8281 gccgatcagg acaatccggg tacgggattg caagaggcgt gtcgaagggt cgtcgaagtc
     8341 gccgtcgatg tcgtgcgccg caacgtcggt gttgtccgcg cggccgcgca atatgaaagt
     8401 atctatccgg agcgatgggg aacgatccgc gccaccggat ccggcatggt cgatctcgcc
     8461 accccgctct tgcttgcgcg gatgggccgc ggccggattc cggcgaagac gcgcagcatc
     8521 ggcttcgcgt ttcagatgat gttcggcacg ttgtgcaacg cggtcctgca caagcctaaa
     8581 cttgtctcgc tcgacgaacc ggaaatggtc gaccgtctgg cgctgacgat gttcctccag
     8641 ttgaagcacg aagccggggc ggaaaagccg gtgaaatagc ccgcggccga gaagggggcc
     8701 gggctacttg gccgggcttg gtttgcctct tggatcgatc ggcgtcgtgc cgcttacgcc
     8761 caaaatatcc tccagcagcc tggcgccggc gagaagctgg gcctcgccgc gcggcgccgc
     8821 caccatctgg agtccgaccg gcaggcctga cgccgtaaag ccgcacggca acgacaacgc
     8881 cgggcagcat accagcgtga tcgcatagac gatcgcgagc cactcgacat agttgtcgaa
     8941 cttctggccg gcgcattcgg cgacatagcg gtgctcgatc ggaaacggcg cgacgatggt
     9001 cgccggcgcc agcagcagat cgtaggtcct gaaaaactca agcgtgcgcg cggccatcgc
     9061 gacgcgctgg ccttcggccc gctcgagctg ctcgaccgtc agcttgaggc cctgttcaat
     9121 gttccagata acctccggct tgagcagatc gcgttttgtc cgcaacagcg ccgctttgct
     9181 gatcgcgaaa tcgaaggcgc gcagcacctg aaagcattcg tgggcctcgc gcaggtccgg
     9241 atgcgcttcc tcgacgatgg cgccggcctc ggcgaagcgc gccgcggctt tccgggtgat
     9301 ggcgacgact tcaggatcga ccggggtgat gccgagatcg ggcgaatagg cgacgcgttt
     9361 cggcttgttg ccggagcgcg cggccgacag gaacgagacc ggcagtgcgg gcagcgacaa
     9421 cggatcggcc ggatgctcgc cgctcatggc atcgagcagc agcgccaggt cctcgacatt
     9481 gcgcgccatc gggccgtgca cggtcagatt gcggtcgatc ttgaaggtgg gcgtatgggc
     9541 gacgcggccg atgctcggcc gcaggccgac gacgccacag aagctcgcgg ggttgcgcag
     9601 cgagccgccc atgtcgctgc catgcgcgag ccacgccatg ccggttgcga gtgcggcggc
     9661 ggcacccccg gacgagccgg cggcggatcg cgacagatcc caggggttga gcgtggcgcc
     9721 gaacacctcg ttgaaagtat tggcgccggc gccgaattca ggggtattcg atttggcgta
     9781 gatcacggcg ccgttgcttt cgagacgttc aaccacgaca tcggatcgtg tggcgatcgt
     9841 atccttgtag atcggcgagc cctgtgtggt ctttacgccc gcgacattgg tgaggtcctt
     9901 gatcggcacc ggcatgcccg ccagcaggcc gcgctcgccg agcggcttct tcatcagcgc
     9961 ctcggcatgg gttcgggcgc ggtcgaagca cagcgtcggc agtgcgttca cttggccgtc
    10021 gacccctgcg atccgctgct ccagtgcgtc gagcagatcg agcggtgtga cttcgctctt
    10081 gttgagtttg tcgacgatgg cgcacgccgt cgcccgcacc agcccttgat caaatgccat
    10141 tgagatctct ccaatcgtaa cgcttctcga tagcggattg tgcagccgtg taaaacagtt
    10201 tccggcaaag tggaaacgag gtagagcagt gcgccgcaat tggagatctt aaagatgagt
    10261 acgccgttcg atgcgcccga ctggcgcgat atgagccagc aagaccgtga cctcggtctc
    10321 aacaacggcg tcgccgtggc cggaagcggc gatatggtcg cgggctggga acggcgctcg
    10381 gcgcagatgc gggcgcggga ttccgatcat ctcgacctca gatacggccc gcgcgagcgc
    10441 aaccggatcg attttttcaa ggctgccgag aaagcgccaa cggttctgtt cattcacggc
    10501 ggctactggc agatgcgagc caaggaggtt tttgcgatat ttgccgctgg cccgctggcg
    10561 catggcatca acgtcgcaac gatcggctac acgctcgccc cggatgcgac gctcgacgag
    10621 atcgtcgccg aaatccacca ggggctggat tatctcgcgg aggaattgcc cgcgctgggt
    10681 ggggacgcga accggatcgt ggtgtccggc tggtccgccg gcgggcatct gaccgcgatg
    10741 gcgctgtcgc atcgctgcgt caaagcggcc atggcgatca gcggcatcta cgatctcgag
    10801 ccgatccgcc acagctatct caacgtcaag ctcgggctcg atgaggcgac gtcccggcgc
    10861 aattcgccgg tcatgcagcc cggcggcgcg atgaagccgc tggcgttggt cgccggagca
    10921 gccgagctgc cgttgctgcg caggcagacc gcggatttcg cgtgccaccg tgccaaatat
    10981 ggattgccgg taatttacga agagatcccc ggcgccgacc actttaccat catgcacgag
    11041 atgatgtcgc cgacggggcg gatcacggcg ttgattagag agcttgtttg agcgcggggc
    11101 gggttagtct gctccctcat tgcgagcgaa gcgaagcaat ccatagctac aaagacaaag
    11161 caagagtggt cgttggatcg cgctaccccc agcccgcgct gagcccgccg tcgaccgtgt
    11221 agatcacccc cgacgtatag ccggagcggt cggaggcgag gaacgccatc aggtcgccga
    11281 tctctcgcgc gtgcgcggga cggccgagcg gcaggccctt ttgaaactcg gtgtagcggc
    11341 tctcgtcacc gaactggttt tttgcgcggg tcttgagcag ggtgatgtgg cggtcggtgc
    11401 cgaccgggcc gggattgatg ccgaccaccc ggatattgtc ggcgaggctc ttgccgccga
    11461 gcgcgcgggt gaaggccatg atcgcggcat ttccggcgct gccgcagatg taattggcgt
    11521 cgaacctttc gccggcggcg ccgatatcgt tgacgatgac gccgtggcca cgcgctttca
    11581 tcgccgcata gatcgcgcgg gtgaggttga catagccgaa cactttcagg tcccaggcgt
    11641 gacgccaggt cgcctcatcg atcttgtcga ccgagccgcc cggaatatcg ccggcattgt
    11701 tgacgaggat gtcgatatca gcggcgtcct tggcgagcct ggcgagatcc gcaggattcc
    11761 gcaggtcgac gatttgcgtc gtggcgtcga tctgatgggc cgagcgcagc cgatcggcga
    11821 gtgccttcag ttgatcgccg ctg
//
LOCUS       DATNOI010000480        17498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_501547,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000480 DATNOI010000000
VERSION     DATNOI010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17498
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501547"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="VN968_19160"
     CDS             complement(<1..1377)
                     /locus_tag="VN968_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HXL31389.1"
                     /translation="MAGESGKKLFVGPRFRRIRQQLGLSQTQIAEGLSISPSYINLIE
                     RNQRPVTAQILLRLAETYDLDLRDLATADEDRFFAELNEIFSDPLFRQIDLPKQELRD
                     LAELCPGVTHSLQRLYAAYTEARRGETLVAAQMADRDEGTRFEANPIERVRDLIEANR
                     NYFPELEQAAENLRDELNVPSEQLFAALSTRLREKHSIVTRIMPVDVMRETLRRFDRH
                     RRQLLISELVDGPGRSFQLAFQMGLAECSTSFETIAGRAGPLDDTPRRLYRITLANYF
                     AAAVMMPYEVFHGAAETLSYDVHVLARRFNAGFEQVCHRLTTLQRPNARGVPFFLLRV
                     DNAGNVSKRFSSGTFPFSKFGGTCPLWNVHSTFDTPDRLLKQVIELPDGTRYFSIAQM
                     VRRPVAPHPQAQPRFAIGLGCEIRHASRLIYATGMDLEKTEGTPIGVNCRLCERENCS
                     QRAEPPITR"
     gene            1555..2862
                     /gene="aceA"
                     /locus_tag="VN968_19165"
     CDS             1555..2862
                     /gene="aceA"
                     /locus_tag="VN968_19165"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HXL31390.1"
                     /translation="MAKTFEQLVPAPAGRFDGISRPYSPAEVEKLRGSVPIAYTLAEK
                     GANRLWESLKKEPYVNSLGAVTGNQAMQQARAGLPAIYLSGWQVAADANTAGAMYPDQ
                     SLYPANAGPELCRRINRTFQRADQIEHSEGGAKIDWFVPIVADAEAGFGGPLNSFEIM
                     KAYIEAGAAGVHFEDQLASEKKCGHMGGKVLIPTAAHERNLIAARLAADVCGTPTFVL
                     ARTDAESAKLITADVDERDREFITGERTAEGFFRLKPGSGLAHCIKRGIAFAKYADLL
                     WWETSTPNLKEAKEFAEAVHRVYPGKMLAYNCSPSFNWEANIDKATIAKFQREIGAMG
                     YKFQFVTLAGFHSLNHGMFELARGYKAEGMAAYSRLQQAEFASEKHGYSATRHQREVG
                     TGYFDLVSTTITGGTSSTTALHDSTEAAQFKAHAEELKIQAAE"
     gene            2875..3084
                     /locus_tag="VN968_19170"
     CDS             2875..3084
                     /locus_tag="VN968_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31391.1"
                     /translation="MMQGSNFWVIGGEFGSMNFHKLVEGSAQVQGPFKTRKEAEDAWR
                     VVSEENRHKAGVRFSIVEEPSRVTA"
     gene            3340..3897
                     /locus_tag="VN968_19175"
     CDS             3340..3897
                     /locus_tag="VN968_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31392.1"
                     /translation="MSEAQNPGSHAAAEARPIRLRDALRQARIEAADRTGVVVDLRDA
                     EVARLEILNEALDPLFAQIPDTVDLFDRGISQGETPRLWIDIVSHVVMGRDKRIYRFV
                     QDSRFGRIVIAESHEIAVVVGAITDYVARRMIERERALVATPMAEPAVTGKPRRGRFW
                     TFALGFIVGVIALFGLALFMALRNF"
     gene            complement(3992..4879)
                     /locus_tag="VN968_19180"
     CDS             complement(3992..4879)
                     /locus_tag="VN968_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXL31393.1"
                     /translation="MAAFTLAHLSDPHLPPPPGPRLADLAGKRALGYLNWKRSRQKVH
                     KRDVLDALVADMQAQRPDHIAVTGDLVNLALEAEFAPARAWLESVGASDRVTVIPGNH
                     DAYVRATQHRFAEVWGNYLDGDAAAGGVTFPSLRRRGPLALIGVSSAVPTPPLMATGW
                     LGRSQLDALRRVLTGLSTEQAFRVLLIHHPLRSNSHIKRLTDSSELLALVKDHGVELI
                     LHGHDHVHSTIWIDGPKRTIPVVGVPSASAVAHGRYPAAAYNLFSVERDGAAWRCEQT
                     VRGIDDTRRVQQLRQVALF"
     gene            5028..5513
                     /locus_tag="VN968_19185"
     CDS             5028..5513
                     /locus_tag="VN968_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXL31394.1"
                     /translation="MTLQELRKMFEPALRRIFHLYWRFARGMTLGVRGVVLDGEDRVF
                     LVKHSYVSGWHLPGGGVEVGETFGDALRRELMEEGRIEMLAEPVLHGVFLNSHVSRRD
                     HVAVYLVRHFSQDRPPEPNREIVACGFFAPAALPAETTKGTRLRISEVLENRRPIATW
                     R"
     gene            5675..6187
                     /locus_tag="VN968_19190"
     CDS             5675..6187
                     /locus_tag="VN968_19190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HXL31395.1"
                     /translation="MSDLSLTILAETAGDALPIERLHQRTFGPGRFVLSAYRLREHVD
                     HLLDLSFTARIGTLLVGSVRQLPILIGDTKALMLGPLTVEPPFRGRGVGRALLDRSLR
                     DAKARGHRLVVLVGDEAYYSRVGFKAIPKGRATMPGPVDYSRLLVNELVDGAFTDVSG
                     AIRPDWSMAR"
     gene            complement(6255..6722)
                     /locus_tag="VN968_19195"
     CDS             complement(6255..6722)
                     /locus_tag="VN968_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31396.1"
                     /translation="MIGIRPFPGAVIAVVMTCVAASPSASDDAAPLSPFQPHVDMKTF
                     MEHVLGPAAAIIWRVNGVVIDEKGEHDLSPKSDEDWEQVVSGAATLAEATNALMIPQR
                     VHDPDWNPHVKKLADAANKAYRAAEAHDLKSVSEVSDQLDGICAACHRHYGLE"
     gene            complement(6724..7329)
                     /locus_tag="VN968_19200"
     CDS             complement(6724..7329)
                     /locus_tag="VN968_19200"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HXL31397.1"
                     /translation="MAADGRPVRRHCLDPGLVRHPVRRTLDCLHAGWIMLKFWDVISY
                     FEDSTLADNIRENDVLFPLIESVHVVSICLVVGSILVVDLRLLGLASVSRPVSRVTSG
                     ILPLTWSAFAIAVASGGLLFISNATKYLANGYFVAKILLICVAGLNMAIFHAISAGDL
                     PKWENEKTLPRQAQLAGGLSILLWVSVVTCGRWIGFTMEVR"
     gene            complement(7227..7733)
                     /locus_tag="VN968_19205"
     CDS             complement(7227..7733)
                     /locus_tag="VN968_19205"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HXL31398.1"
                     /translation="MTDVGLSEICRWLAATSLSHTIQETGWIIPALQTIHILGVALIF
                     SSAILVDLRIWRLLERDVPLPEVAQRFLPAIWPVLLILLITGSLLIVGEPRRSLLNFT
                     FYLKMALLAVAIMLTVGLQRSISSSPNFWDRDRWRRMAGRFAATVSILVWCGILFAGR
                     WIAYTQAG"
     gene            complement(7738..8133)
                     /locus_tag="VN968_19210"
     CDS             complement(7738..8133)
                     /locus_tag="VN968_19210"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HXL31399.1"
                     /translation="MVSPKTAFVVASLTLGSGGIAAAHHSNAAYDLEHPKTVEATVKT
                     VNWTNPHITFVVETDAKDAAPASTWVFEVSSPGVLTRSGWTKRSLQPGDHAVFRYAPL
                     RDGNPGGFLIKVTLPSGQELSYTLMPAEQ"
     gene            complement(8146..8925)
                     /locus_tag="VN968_19215"
     CDS             complement(8146..8925)
                     /locus_tag="VN968_19215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31400.1"
                     /translation="MNIVAKKLAIVFCAAALVEAFVASRALAEPPDFSGVWLPDVKDQ
                     KRQETANVLPWKPEILPQIQHMIAEEKAGRPFLVLSHCLPHGMPSWMLITHNAFELLV
                     TPGRITMLGEVDGNRMRRIYLDGRPHPEDPDLTLHGHSIGHWDGDTLVIDTVAIAPQA
                     YIAISEAVGIPNNGDMHILERLHLAKPDVLYDDLEITAPKVLNASWKTTRIFRRYPER
                     HYEITEGECAQEDLTPGKDQFGNDIFVPNPQGLYGSVHTVK"
     gene            9027..9395
                     /locus_tag="VN968_19220"
     CDS             9027..9395
                     /locus_tag="VN968_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31401.1"
                     /translation="MLVTAIAKEKQSGGARLHHSERVAFHRQRVDQKKLRIAAADLSV
                     LGFKAHFDHAPSIRRLIMDHHAVYVGRQKKSCLASSTATGTVESSSKKALEEPGFTTC
                     ACTPVGEPDLVLAGLPPMTP"
     gene            9451..10110
                     /locus_tag="VN968_19225"
     CDS             9451..10110
                     /locus_tag="VN968_19225"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXL31402.1"
                     /translation="MPRSGLMLLLVGLLGFMLGGCMQATLAPTSDASLTPRDRQLLAH
                     PPYARADIPESYRRHIVDYPRKEAPGTILVDTDARYLYYVLPGGKAIRYGVAVGEEAM
                     AFSGVATVGRMTEWPDWIPTPDIQARLGPYPARVPGGPANPLGARALYLYAGNKDTLY
                     RIHGTNQPEYIGQAISSGCIRMTNEDVIDLFDRVKQGATVVVLAPAQSGRIGSLSGSR
                     S"
     gene            10276..10611
                     /locus_tag="VN968_19230"
     CDS             10276..10611
                     /locus_tag="VN968_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31403.1"
                     /translation="MIIAMITVRMVQPAVYEIINVVTMRHLFMSAVWTVSVCAVDLRR
                     AFHGICGVNCDHMFVHVIPVHMVEMAVVKIIHMAVMANRGVPAFRAMLMSVVGVVFLG
                     ACGHRQCLL"
     gene            10780..11091
                     /locus_tag="VN968_19235"
     CDS             10780..11091
                     /locus_tag="VN968_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31404.1"
                     /translation="MAAFFFPRKISNRRRDAALQLKEHALTVINADDETVVSVSERDC
                     RDPGCSGVQTIVLVMHPRRPTEAVKIDKPLEQITQTELSEALAPLAAQTGLPEPPLKP
                     K"
     gene            11160..12131
                     /locus_tag="VN968_19240"
     CDS             11160..12131
                     /locus_tag="VN968_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXL31405.1"
                     /translation="MQAALNQTPVTVITGYLGSGKTTLLNRILTEAHSKRYAVIVNEF
                     GEVGIDNDLIVNAEEEIFEMNNGCICCTVRGDLIRILGGLMRRRGRMDGIIVETTGLA
                     DPAPVAQTFFVDEDVRRRTKLDAIVTVVDAKHLLGEIDRAPEAQEQLGFADIVLLNKT
                     DLVTEDDLASVEARIRRINPYAGIHRTERCGIDLAQVLGRDAFNIDRVLEIVPDFLIE
                     THEHEHDDEIGSLSLTADRPLDPERFVPWIQKVTQDFGTDILRMKGIIAMKDDDQRFV
                     VQGVHMLLEGGSQRPWKSDEKRDSRLVFIGRGLPKERLREEFESCRV"
     gene            12316..13227
                     /gene="mepA"
                     /locus_tag="VN968_19245"
     CDS             12316..13227
                     /gene="mepA"
                     /locus_tag="VN968_19245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HXL31406.1"
                     /translation="MLALSIVALAATATFGQDKGSLDPKPLPPLANPNDPKLGAKELF
                     GRIASPAPMTTHSIGFYAKGCLAGAEALPINGETWQVMRLSRNRNWAHPDMVALLERL
                     SVKAHEVAGWPGLLVGDMSQPRGGPMLTGHASHQVGLDADIWLTPMPNRELSRNEREE
                     MSATSMVRTDRLDIDPAVWTPNHLLVIRAAAQEARVERIFVNAAIKKALCREAKGDRS
                     WLAKVRPMYGHDYHFHVRITCPATDNDCTPQPPPLEGEACSAADFAYWFSDPVIHPKS
                     SSAPSEPRPPMTLAQLPAACRQILLAP"
     gene            13340..13798
                     /locus_tag="VN968_19250"
     CDS             13340..13798
                     /locus_tag="VN968_19250"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL31407.1"
                     /translation="MLIDWEGVGPERRFEVRVPNVLQAGKFYRDVFGAEETFRQQTHN
                     GKVFRLGFVIGRIGFAISSEGDIEQERPLLSSIAEELGSALVAVILRVEDPDRIASAA
                     RQAGSGLARAHELDLITVVTDPFGGYWALVKREPVPVANLSGLAVDREGR"
     gene            complement(13991..15151)
                     /locus_tag="VN968_19255"
     CDS             complement(13991..15151)
                     /locus_tag="VN968_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HXL31408.1"
                     /translation="MRMTRMKTDRHIALSCLAIMAATMVVDGALQSALAQNPFGGPPA
                     APDAQVGGIVGWLLTKQSEFYREMSSTIRAAKADGSAVWTLLAISFGYGMFHAAGPGH
                     GKTVISSYLVANEETARRGIVLSFVSALMQSLIAVLIVGLGAWLLNVTAKTMCGAERV
                     IEIASYTLIAAFGSRLVWTKGGGLIRALQSSHGKFEPRLAPALAHAAIQRHHGHHSHG
                     HGDHGHHHHAHAPAHPTVAPDHVPEHEHAEHHDHGHVHDEHCGHSHGPEPAQLGGPGG
                     WRRGFSAVLTVGIRPCSGAILVLVFALAQGMFWAGIAATFVMGLGTAITVAAIAILAV
                     SAKGLARRIGAGREGGGALLMRGVEFGAAGLVLLFGVGLLLGYIAAERVTCF"
     gene            complement(15148..15789)
                     /locus_tag="VN968_19260"
     CDS             complement(15148..15789)
                     /locus_tag="VN968_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HXL31409.1"
                     /translation="MTWSIQLLAFVAAVALSNSVSPAAAHPHVWVTAATELIYAPDGS
                     ITSVRHAWAFDDMFSTYALQGVATKRKGIYTREELAPLAQTNVESLKEYGFFTVAKVD
                     GKKQPFLDPTDYFLEYKNAALVLHFTLPLKTPIKTRQLAVEIFDPTYFVDFALQKQDP
                     IRLVGAPASCSMEIQRPNDGAAGAQKLDESSFLNGSNGNYGVMFANKITVDCP"
     gene            complement(15892..16845)
                     /gene="exbB"
                     /locus_tag="VN968_19265"
     CDS             complement(15892..16845)
                     /gene="exbB"
                     /locus_tag="VN968_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tonB-system energizer ExbB"
                     /protein_id="HXL31410.1"
                     /translation="MNKSLSRAVYSGIVALAIFSTALPSLAQQQTPQTPALSQTPPAQ
                     AAPESQAPAPSAAGQSTPAAGDVSAAPVDGGRSLKSTTVALRELSPWSMFLSADILVK
                     AVMIGLAFASLVTWTVFLAKMIELSLAQRQLRAALGKISDARSMAEAQFALGARGSVL
                     SRLLSAAMREARLSAGISSDSGIKERAASSFAEIVRAEARRIRLGMGVLATIGATSPF
                     VGLFGTVWGIMNSFIGISKSQTTNLAVVAPGIAEALLATAMGLVAAIPAVVIYNHFSR
                     VTKGYLELVSRSSGAAGRLLSRDLDRAQVSSPGSAPSRAAK"
     gene            complement(16893..>17498)
                     /locus_tag="VN968_19270"
     CDS             complement(16893..>17498)
                     /locus_tag="VN968_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligand-gated channel"
                     /protein_id="HXL31411.1"
                     /translation="WPVPGLEVGLDGYLKLARDLLDDGQFGQAYVLDGFNYAKANNIG
                     LEFKALYAMGNLRMYGNVAWARQLGTNVVSNQFLFGADELAYIANHYIYTDHSQVLTA
                     SAGASYLWKDTRFSASMIYGSGLRSGFVNTDHLPSYTQVNMGVSHDFFIVAPNKPTTL
                     RFDVVNVFDKIYEIRDGSGIGVFAPQFGPRRGFFVGISQKI"
BASE COUNT         3524 a         5249 c         5393 g         3332 t
ORIGIN      
        1 ccgcgtgatc ggcggctcgg cccgctggct gcagttttcg cgttcgcaaa gccggcaatt
       61 gacgccgatc ggcgtgccct cggttttttc cagatccatc ccggtggcgt agatcagccg
      121 cgaggcgtgg cggatttcgc accccaaccc gatcgcaaag cgtggttgag cttgcggatg
      181 cggcgccacc ggccggcgca ccatctgcgc gatcgaaaaa tatcgggtgc cgtcgggcaa
      241 ttcgatcacc tgcttgagca ggcggtccgg cgtgtcgaag gtcgagtgca cgttccacaa
      301 cgggcaggtg ccgccgaatt tcgaaaacgg gaaggtaccg gaagagaatc gcttcgaaac
      361 attgccggcg ttgtcgacgc gcagcaggaa gaatggaacg ccgcgcgcgt tgggccgctg
      421 cagcgtggtc aggcgatggc agacctgctc gaaaccggca ttgaaacgcc gcgccagcac
      481 gtggacatcg tagctcaggg tttccgccgc accatgaaac acctcatagg gcatcatcac
      541 ggcggctgcg aaatagttcg ccagcgtaat ccgataaagc cggcgcgggg tgtcatcgag
      601 cggccccgcg cgacccgcga tggtctcaaa actggtgctg cattcggcaa gccccatctg
      661 gaaggcgagc tggaacgagc ggccggggcc atcgaccagt tcggagatca gcaattgccg
      721 gcgatggcga tcgaaccgcc gcaacgtctc gcgcatgaca tcgaccggca tgatgcgggt
      781 gacgatcgaa tgcttttcgc gcaaacgcgt gctgagtgcc gcaaacagct gctcggacgg
      841 cacgttcaat tcgtcgcgca gattttccgc cgcctgttcg agttcgggga aataattgcg
      901 gttggcctcg atcaggtcgc ggacgcgctc gatcggattg gcctcgaagc gcgtgccttc
      961 gtcgcggtcg gccatttggg cggcgaccag cgtctcgccg cggcgggcct cggtataggc
     1021 ggcgtacagc cgttgcaatg aatgagtgac gccggggcac aattcggcca gatcgcgcag
     1081 ttcctgcttc gggaggtcga tctgccggaa cagcggatcg gagaaaatct cgttcaattc
     1141 ggcgaaaaac cggtcctcgt cggcggtggc gagatcgcgc agatcgaggt cataggtctc
     1201 cgccagccgc agcaggatct gcgccgtcac cgggcgctgg ttgcgctcta tcaggttgat
     1261 atagctcggg gaaatactga gcccctcggc gatctgggtc tgcgacaggc ccaattgctg
     1321 gcggatccgc cgaaatcggg gaccgacaaa cagcttcttt ccggattcac cggccatggc
     1381 agcctcgctg accttttttg tgacaaaaat tacaaagtaa catctgcgac atgtacggat
     1441 gttacatgac atcaccatta ggatgcaagg tgtctatacc aattaggaag tttagcgttt
     1501 agctcacgcc acgtatcgca atgcattgtc acgaatgtcg attggagaac taagatggcg
     1561 aagacctttg aacagctcgt tccggcgccc gcagggcgct tcgacggcat cagccgtccc
     1621 tacagcccgg ccgaggtgga gaagctgcgc ggctccgtgc cgatcgccta cacgctcgcc
     1681 gagaagggtg ccaatcggct gtgggagtcg ctgaagaagg agccttacgt caattcgctc
     1741 ggcgccgtca ccggcaacca ggcgatgcag caggcgcgcg ccggtctgcc cgcgatctat
     1801 ctgtcgggct ggcaggtcgc cgccgacgcc aacaccgcgg gtgcgatgta tcccgaccag
     1861 agcctttatc ccgccaatgc cggtccggag ctttgccgcc gcatcaaccg cacgtttcag
     1921 cgcgccgacc agatcgagca ctccgaaggc ggcgccaaga tcgactggtt cgtgccgatc
     1981 gtggccgatg ccgaagccgg ttttggcggc cccctgaatt cgttcgagat catgaaggcc
     2041 tatatagagg cgggtgcggc cggcgttcac ttcgaggacc agctcgcgtc ggaaaagaag
     2101 tgcggccaca tgggcggcaa ggtcttgatc ccgaccgcgg cgcatgagcg caatctgatc
     2161 gcggcgcgtc tggccgccga cgtctgcggc acgccgactt ttgtcctggc gcgcaccgat
     2221 gcggaaagtg cgaaactgat caccgccgat gtcgacgagc gcgaccgcga gttcatcacc
     2281 ggcgagcgca ccgcggaagg ctttttccgc ctcaagcccg gcagcggtct cgcccattgc
     2341 atcaagcgcg gcatcgcgtt cgcgaaatac gccgatctgc tctggtggga gacctcgacg
     2401 ccgaacctca aagaggcgaa ggaattcgcc gaggccgtac acagggtcta tccgggcaag
     2461 atgctggcct ataactgctc cccgtcgttc aactgggaag ccaacatcga caaggccacc
     2521 atcgcaaaat tccagcgcga aatcggcgcg atggggtata agttccagtt cgtgaccttg
     2581 gcgggcttcc actcgctcaa ccacggcatg ttcgagctgg cgcgcggcta caaggccgaa
     2641 ggcatggcgg cttactcccg cctgcagcag gccgagttcg catccgagaa gcacggttat
     2701 tcggcgacgc gccatcagcg cgaagtcggc accggctact tcgatctggt gtcgaccacc
     2761 atcaccggcg gcacctcgtc gaccacggcc cttcacgact cgaccgaagc cgcacagttc
     2821 aaggcgcacg ccgaggaact caagattcaa gcggctgaat aacaggagga tgtcatgatg
     2881 caaggcagca atttctgggt gattggcggc gagttcggtt cgatgaactt ccacaagctc
     2941 gtggaaggct cggcccaggt gcagggtccg ttcaagaccc gcaaggaggc cgaggatgcc
     3001 tggcgcgtgg tatccgaaga gaaccgccac aaggccggcg tgcgcttttc catcgtggaa
     3061 gagccttccc gcgtcacggc ctgacaccgg ccggcggccg gccaacgtta agaagaagac
     3121 gtccaaggac aagagcggtc ccaccggttt tccggcgggg ccgttcgcgt ttttagccat
     3181 ggtgttttcg agcgacgtgg aagccggttc gcgccaggaa aacccgtcaa aacaagcgtg
     3241 gaggccgcaa gcctttggag ataaacggcc tttgcggaca gaatggttac tgataggttg
     3301 agttcattcg gacccgtcag actcaaaggc aaccagcgga tgtcagaggc gcaaaatccc
     3361 ggaagccatg ccgccgccga ggcgcgtccg atcaggctgc gcgacgcgct gcggcaggcg
     3421 cggatcgagg ctgccgatcg caccggcgtc gtggtcgacc tgcgtgacgc cgaagtggcg
     3481 cggctcgaaa tcctcaacga ggcgctcgat cccttgttcg cgcagattcc cgacacggtc
     3541 gatctgttcg atcgcggcat cagccagggt gaaacgccgc ggctgtggat cgacatcgtt
     3601 tcgcatgtcg tgatgggacg cgacaagcgc atctatcgct tcgtgcagga cagccgcttc
     3661 ggccgcatcg tgattgccga atcccacgag atcgcggtgg tcgtcggcgc catcaccgat
     3721 tatgtcgccc gccgcatgat cgagcgcgaa cgggcgctgg tcgcaacccc gatggcggag
     3781 ccggccgtga ccggcaagcc gcgccgcggc cggttctgga ccttcgcgct cggcttcatc
     3841 gttggcgtga tcgcgctgtt tggtctcgcg ctgttcatgg cgctgcggaa tttctagtcg
     3901 tcgttccggg gggcgcgaac tcggaatctc gagattcccc gatgcgcaat tgcgcatctg
     3961 aggtctggtc cttcggacca tcccggaatg actagaacaa tgcaacttgc cgcaattgct
     4021 ggacgcgccg cgtatcgtcg atgccccgca cggtctgttc gcaccgccac gccgcgccat
     4081 cacgttcgac cgaaaacaga ttgtaggccg cggcaggata gcggccatgc gcgaccgcgg
     4141 aggccgaagg cacgccgacc acgggaatgg tgcgcttcgg tccgtcgatc catatcgttg
     4201 aatgaacgtg atcatgcccg tgcaggatca actccacgcc gtgatccttc acgagggcga
     4261 gcagttcgga tgaatcggtc agccgcttga tgtgcgaatt cgagcgcaac gggtgatgaa
     4321 tcagcaggac acgaaaagcc tgctcggtgg acagacctgt cagaacgcgc cgcagcgcat
     4381 caagctggct gcgcccgagc catcccgtcg ccatcagcgg cggcgtcggg accgccgagg
     4441 atacgccgat cagcgccagc gggccgcgcc ggcgcagcga cggaaaggtg acgccgccgg
     4501 ccgcagcgtc gccatcgaga taatttcccc aaacttccgc aaagcggtgt tgcgtcgcgc
     4561 ggacataggc gtcgtgattg cctgggatca ccgtgacgcg atcggatgcg ccgacgctct
     4621 ccagccatgc ccgcgccggc gcgaactccg cttcgagcgc gagattgacg aggtcgccgg
     4681 tgaccgcgat gtgatccggc cgttgcgcct gcatgtccgc caccagcgca tcgagcacgt
     4741 cgcgcttgtg gactttttgc cggctgcgtt tccagttcag atagccgagc gcgcgcttgc
     4801 cggcgaggtc ggcaagccgc gggcctggcg gcggcggaag atgcggatcg gacagatggg
     4861 ccagcgtgaa tgccgccatc agccgcatcc gctgctgttg cccgtgcgat ggcgccgctg
     4921 cacgatcatc tcgtgatacc ctgtcaattc cgacaatgta ttggcaagcc ggccgccgca
     4981 aggcaaggat caaacgcgat gtctggatat cgggaatttc ggtggcgatg accctgcaag
     5041 agttgcgaaa gatgtttgag ccggcactgc ggcggatttt ccatctctat tggcggttcg
     5101 ctcgcggcat gacgctcggg gtccgcggcg tggtgctcga cggcgaggac cgcgtgtttc
     5161 tggtcaagca cagctatgtt tccggctggc acctgccggg cggcggcgtc gaggtcggtg
     5221 aaacattcgg cgatgcgctg cgacgcgagc tgatggaaga gggaagaatt gaaatgctgg
     5281 ccgaaccggt cctgcacggt gtgttcctca acagccacgt ttcccgccga gaccacgtcg
     5341 cggtctatct ggtccgtcac ttcagccagg atcggccgcc cgagccaaac cgcgagatcg
     5401 tcgcttgcgg tttcttcgcg ccggctgcgc tgccggctga gacgaccaag ggaacgcggc
     5461 tgcggatatc ggaagtgctc gaaaacaggc ggccgatcgc cacctggcgt tagagaacgg
     5521 tccatatgat cggggttcaa aacgctaata atcgttatca agctgctggc ctatctgtcc
     5581 caagccccga tcaacaagga ctgatgtggc ttaaaaaaat aggccggacc ggcgatagac
     5641 cgcgcgtccg ccagatgcta tcccgcgcgt cagcatgagc gacctttctc ttacaatttt
     5701 ggccgaaacc gccggcgacg cgctgccgat cgagcgcctg caccagcgca ccttcgggcc
     5761 cggccgcttc gtgctgagcg cctatcggct gcgcgagcat gtggatcacc tgcttgatct
     5821 gtcgtttacg gcccgcatcg gcacgctgct ggtgggctcg gtgcgccaac tgccgatact
     5881 gatcggcgac accaaggcct tgatgctggg cccgctgacg gtcgagccgc cgtttcgcgg
     5941 ccgcggcgtc ggccgcgcgc tgctggatcg ctcgctccgg gacgccaaag ccaggggaca
     6001 tcgcctcgtg gtgctggtcg gcgacgaagc ctattacagt cgtgtcggct tcaaggccat
     6061 tccgaaggga cgggcgacga tgccggggcc ggtcgactac agccgccttc tggtgaacga
     6121 actcgtggat ggcgcgttca ccgacgtttc cggcgcgatc cgcccggact ggagcatggc
     6181 gcggtagctc ggcgccatgt gggacgacaa atccgaagaa acggccagcg aaatcgaccg
     6241 cacgccgttg ccatttattc aagtccatag tgacggtggc aggccgcgca aatcccgtcg
     6301 agctggtcgc tgacctctga gaccgatttc aggtcatggg cttccgcggc gcggtaggcc
     6361 ttgttcgccg cgtctgccag tttcttgacg tgagggttcc agtccggatc gtgcacccgc
     6421 tgcggaatca tcagcgcgtt cgtggcttcc gccagcgtcg ccgcgccgct cacgacttgc
     6481 tcccagtcct cgtccgattt gggcgagaga tcatgctcgc ccttttcgtc gataaccacg
     6541 ccgttaactc tccagatgat cgccgcggcc ggaccaagga catgctccat gaaggtcttc
     6601 atgtcgacat ggggctggaa gggcgacagc ggggccgcgt catcggatgc cgacggcgac
     6661 gcggccacac aggtcatgac gacagcgatc acggcaccgg gaaagggacg gatgccgatc
     6721 atctcagcga acctccatgg tgaatccgat ccagcgcccg caagtgacca ccgagaccca
     6781 cagcagaata gacaatcccc cggcgagctg tgcctgacgc ggcaacgtct tctcgttctc
     6841 ccacttcggc agatccccgg cgctgatggc gtgaaagatc gccatgttca ggccggccac
     6901 gcaaatgaga agaatcttgg caacgaagta gccgttcgcc agatatttgg tggcgttgga
     6961 aatgaagagc aggccgcccg aggcaaccgc aatcgcaaac gcgctccatg tgagcggcaa
     7021 gattccgctg gtgacgcggc tcaccggccg gctaacggag gcaagcccaa gcaatctcag
     7081 atcgacaaca agaatggagc caacgacgag gcaaatcgaa accacatgca ccgattcaat
     7141 aaggggaaac agcacatcgt tctcgcgaat attgtctgcg agtgtgctgt cctcgaaata
     7201 tgagataacg tcccaaaact taagcattat ccagcctgcg tgtaagcaat ccagcgtccg
     7261 gcgaacagga tgccgcacca gaccaggatc gagacagtgg cggcgaaccg gcctgccatc
     7321 cgccgccatc gatccctgtc ccaaaagttt ggcgatgacg atatcgaccg ttgcagcccg
     7381 acggtcagca tgatggcgac agccagcagc gccatcttga gatagaaggt gaaattgagc
     7441 agcgaacgtc gcggctcgcc gacgatcagc agacttcccg taatcaagag gatgagcagg
     7501 accggccaaa tggccgggag gaagcgctgc gcgacttccg gcaacggcac gtcgcgctcg
     7561 agcagccgcc agattcgaag gtcgacgagg atcgcggagg aaaagatcag cgcaacgcca
     7621 agaatgtgaa tggtctgcag cgccggaata atccagccgg tctcctggat cgtgtgactg
     7681 agcgatgtcg ccgccagcca cctgcaaatt tcgctcaagc cgacgtctgt catcacatta
     7741 ttgttcggcg ggcatcaggg tgtaactcaa ctcctgacca ctcggcagag tgacttttat
     7801 caagaacccg ccaggattgc cgtcgcgaag cggagcatag cggaaaaccg cgtgatctcc
     7861 gggctggagc gagcgcttgg tccagcccga gcgtgtcaaa actccggggc tcgagacctc
     7921 gaagacccac gtgctcgccg gcgcggcgtc cttggcgtcg gtctcgacga cgaaggtaat
     7981 gtgaggattg gtccaattga cggtcttgac ggtggcttcc acggttttgg ggtgctcgag
     8041 atcgtatgcc gcattggaat gatgcgctgc tgcgataccg ccagatccca gcgtcaggct
     8101 cgcaacaacg aaagccgttt taggcgatac catctcaatc atcccctact tcactgtgtg
     8161 gaccgaaccg tacaggcctt gcgggtttgg aacgaagatg tcgttgccga actgatcctt
     8221 tcccggggta aggtcttcct gagcgcattc accttcggta atctcgtaat gacgctcggg
     8281 atagcgacgg aagattcgcg tcgttttcca gcttgcgttc aggaccttcg gcgcggttat
     8341 ttcgaggtcg tcgtaaagca cgtccggctt cgccagatgc agccgttcga ggatgtgcat
     8401 gtcgccattg ttgggtatgc cgaccgcctc gctgatcgca atataggctt gcggcgcgat
     8461 cgcgacggtg tcgatcacca gcgtgtcgcc gtcccaatgc ccgatcgagt ggccgtgaag
     8521 ggtcagatcg gggtcttcgg gatgcggacg gccgtccaga tagatacgtc gcattcgatt
     8581 gccgtcgact tcgcccagca tggtgatgcg gccgggggtc accagaagtt cgaacgcatt
     8641 gtgcgtgatc aacatccagc tcggcatgcc gtgcggcaga caatggctga gaacaaggaa
     8701 aggtcgaccg gctttttctt cggcgatcat gtgctggatc tgcggtagaa tctccggctt
     8761 ccaaggcaag acattcgccg tttcctgacg cttctggtct ttcacgtccg gaagccatac
     8821 gccgctgaaa tcgggcggct ccgccaaggc ccgggaagcg acgaacgcct cgaccagcgc
     8881 cgcggcacaa aaaacgatcg cgagcttctt cgcaactata ttcacgccga ctaaccgtta
     8941 tttttgacgc gaggcgttat agtcagatcc acagcacgtt cgaagtcgca catgccgagt
     9001 cgctgaacaa ttttggtgcg ccgcatatgt tagtgaccgc aatcgcaaaa gaaaagcagt
     9061 ccggaggcgc tcgacttcac cattccgaga gggttgcttt ccatcgccag cgagtggatc
     9121 agaaaaagct gcggattgcc gcggcggatt tgtctgttct tggattcaaa gcgcacttcg
     9181 atcatgcgcc atcaatccgg aggttgatca tggatcacca cgccgtctac gttggccggc
     9241 aaaaaaagtc gtgccttgca tcgtcgacgg caaccggcac agtggaaagc tcctcgaaga
     9301 aagctctgga agagccgggc ttcaccacct gcgcgtgcac gccggtcggc gaacccgacc
     9361 tcgttctcgc aggcttgcct cccatgacgc cttgatttct gcactattcg agcagcaaat
     9421 ggagccgtgt aacgctttgg aggacaggtg gtgccgaggt ccgggctcat gttgttgctg
     9481 gtggggctgc tcgggttcat gctcggcggt tgcatgcaag cgacgctcgc accgacatcg
     9541 gatgccagtt tgaccccaag ggaccggcag ttgctggccc atccgcctta tgcgcgggca
     9601 gacattccgg agtcctatcg ccggcatatc gtcgactatc ctcgcaaaga ggcgccggga
     9661 acgatcctgg tcgataccga cgcgcgttac ctctattacg tcctgccggg aggtaaagcg
     9721 atccgctacg gcgtggcggt gggagaggaa gcgatggcct tctccggtgt cgctacggtt
     9781 ggccgcatga ctgaatggcc tgattggatc ccgacgccgg acatccaggc acggctcggc
     9841 ccttacccgg cgcgcgttcc cggaggtccg gctaatcctc tgggtgcgcg ggcgctctac
     9901 ctttacgcgg gcaacaagga caccctgtac cgcatccatg gcaccaacca gccggaatat
     9961 atcggccaag ccatatcatc cggctgcatc cgcatgacta acgaggatgt catcgatctc
    10021 tttgatcggg tgaagcaggg tgcgaccgtc gtcgtcctcg cgccggcgca gagtggacgg
    10081 atcgggtccc tctcaggttc tcgcagctga tcagttgctc gttgggaggg tctgtgactc
    10141 cccccaaagc tgacttgcgc gattggtgcg aagccaacgt tcggtcaaat gtctgctttg
    10201 cgccacaggc gtttcggtgc aggtcggttt ttgactgcgc agcgtgggcg cgcctcaggc
    10261 taactgcatg ggcccgtgat catcgcaatg atcaccgtgc ggatggtgca gccggccgtc
    10321 tacgagataa tcaacgtggt caccatgcgg cacctcttca tgtccgcagt ttggaccgtg
    10381 tccgtgtgcg cagtggacct gaggcgtgca tttcacggga tttgtggtgt caactgcgat
    10441 cacatgttcg tccacgtgat ccccgtgcat atggtggaga tggccgtcgt gaagataatc
    10501 cacatggccg tcatggcgaa ccgcggtgtg cccgcatttc gggccatgct tatgagtgtg
    10561 gttggcgtgg tgtttctcgg tgcatgtggc catcggcaat gcctcctgtg agcgtttggt
    10621 tgcccatcga tgccccacga ttcccatcca taagagaata gttgcggaac cccgcgatgc
    10681 cagggttcgc aacaagccga ttgatctccg gaatgtaatg ttataacatt cagccttcac
    10741 aacgacgcca aattggcgcc gtaaggctga cggatccaca tggccgcttt tttcttcccc
    10801 cggaaaattt caaatcgacg aagagatgct gcgcttcaat tgaaagaaca tgcccttacc
    10861 gtcatcaacg ccgatgacga aaccgtagtc agcgtcagcg aacgcgattg ccgcgatccc
    10921 ggctgcagcg gcgtccagac catcgtgctc gttatgcatc cgagacgtcc aaccgaggcc
    10981 gtcaaaatcg acaagccgct tgagcagatc acccagacag agctttccga ggcgcttgcg
    11041 ccattggcgg cacaaaccgg tctgccggag cctccattga aaccgaaatg accatgctgt
    11101 gcccgcgcat cgacgttggc cggcccgcag caatatcaac atcagcaaag gaaaatccca
    11161 tgcaggcggc tttgaaccag actcccgtca ccgtgatcac cggttatctc ggttctggaa
    11221 agaccacgct gctcaaccgg atcctcacgg aggcgcacag caagcgctat gccgtcattg
    11281 tcaatgaatt cggcgaggtt ggaattgaca atgatctcat cgtcaacgcg gaagaagaaa
    11341 ttttcgaaat gaacaacggc tgcatctgct gcacggtgcg tggcgatctc atacgaattc
    11401 tgggcggcct gatgcggcgc agaggccgca tggatggtat catcgtggag acgaccggcc
    11461 tcgccgatcc agcgcccgtc gcgcagacat tctttgtcga tgaagatgtc cgccgccgga
    11521 ccaagctcga tgccattgtg accgtagtgg acgccaagca ccttcttggt gagatcgatc
    11581 gcgctccgga ggcgcaggag caacttggtt tcgccgacat cgtgctgctc aacaagaccg
    11641 acctggtcac ggaagatgac ctcgcctcgg tcgaggcgcg cattcgccgg atcaatccct
    11701 atgccggtat tcatcgcacc gagcgctgcg gcatcgatct cgcccaagtg ctcggccgcg
    11761 atgccttcaa catcgaccgt gttctcgaga ttgtcccgga ttttctcatc gaaacccacg
    11821 aacatgaaca cgatgatgag atcggcagcc tgtcgctgac cgccgaccgc ccgctcgatc
    11881 ccgaacgctt tgttccctgg attcagaagg tcacgcagga tttcggaaca gacatccttc
    11941 gaatgaaggg catcattgcg atgaaggatg acgaccagcg ctttgtggtc cagggcgtgc
    12001 acatgcttct cgaaggcggt tcccagcgtc cttggaaatc ggatgaaaag cgcgacagtc
    12061 gtctggtgtt catcggaagg ggactgccca aggagaggct cagggaagaa tttgagtcct
    12121 gtcgcgtctg atcttcgtca cattcgcaat tggactggac atgatcgcaa ttgcagatga
    12181 actgacgcaa cttacgaacc gagtaagcgc ttgcgggcct tcgaggatcg gatcgacaat
    12241 gatttgggtt tacccaagag agggcctgaa taataaagcc aggtaagcaa catgcgtact
    12301 cgggcgtttt tgagcatgct ggctttgtcc atcgtggctc tggctgcgac ggccaccttc
    12361 ggccaggata agggcagtct tgatccgaag ccgctgccgc cgctggctaa ccccaacgat
    12421 ccaaagctgg gagcgaaaga gctttttggt cgcatagcat cacctgcgcc gatgacaact
    12481 cattcaatcg gcttctatgc caagggttgt cttgccggcg cggaggcact tccgatcaat
    12541 ggcgagacct ggcaagtcat gcggctgtcg cgcaatcgca attgggcgca tcccgatatg
    12601 gtcgctctgc tggagcgatt gtccgtcaaa gcgcacgagg ttgcgggctg gcctggcttg
    12661 cttgtcgggg acatgtcgca accacgcggc ggaccaatgc tgaccgggca tgcaagtcat
    12721 caagttggac ttgatgccga tatctggttg acgccgatgc cgaaccggga attgtcgcgc
    12781 aacgagcgcg aagagatgtc cgccaccagc atggtccgga ctgaccggct cgacatcgat
    12841 cccgcggttt ggacaccgaa tcatctcctg gtgattcgcg cagccgctca ggaggcgcgg
    12901 gtggagcgta tcttcgtgaa cgccgcgatc aagaaggcgc tatgccggga ggccaaggga
    12961 gatcgcagct ggctcgcaaa ggttcgaccg atgtatggtc atgattatca cttccatgtc
    13021 cggataacat gccccgcgac cgacaatgat tgcacgccgc agccgccgcc attggaaggt
    13081 gaggcttgta gcgctgccga tttcgcctac tggttcagcg atcccgtcat tcacccgaag
    13141 tcgtcaagtg cgccgtctga gccaagacca ccgatgaccc tcgcgcagtt gccggccgct
    13201 tgccgtcaaa ttctgctggc gccctgacat tccccgactg ttctgcaatt agccgcagag
    13261 attgaaaaca gaacgtccaa catgatttcg agggttcttc atgacgacgc gagctacacg
    13321 ggaagtgagt tggaacggaa tgctgatcga ttgggaaggg gtcgggcccg aacgacgatt
    13381 tgaagttcga gtgccgaatg tcctgcaggc gggtaaattc tatcgagatg ttttcggagc
    13441 tgaggaaacg ttccggcagc aaacgcacaa cggcaaagtg tttcgactgg gcttcgtcat
    13501 cggacggatt ggattcgcta tctcttcgga aggcgatatc gagcaggaac ggcctctcct
    13561 ctcttcaatc gctgaggaat tgggatccgc ccttgtggcg gtcatactac gagttgaaga
    13621 tccggatcgt attgccagcg ccgcacggca ggccggcagc ggacttgcaa gagctcatga
    13681 gctggacctc atcacggtcg ttaccgatcc cttcggtggt tattgggcat tggtcaagcg
    13741 ggaacctgtc cctgtcgcga acctatccgg cttggcagtc gatcgagagg gccgatgatc
    13801 gtgcgcaatg agggatctct gcagctctct ggttaagcgg caccagatcg ttctgcggcg
    13861 tgacggtcac atcaatgtct gtaatggaca cttggctgac gtcggcgttt cggcggatgt
    13921 ccgctttgca ccaatggagg cgacgctccc tggaagtcgg gttttggacc gaaggcgcga
    13981 cattcgcttg tcaaaagcag gtcacgcgct cggcggcgat gtagcccagc agcaatccga
    14041 cgccaaacaa cagcaccagt ccggcggcgc cgaactcgac gccacgcatc agcagtgcac
    14101 cgccgccctc gcggccggcg ccgatccgcc gtgccagccc ttttgcggag accgcaagga
    14161 tggcaatggc cgcaacggta atcgcggtgc caagccccat cacgaacgtg gcggcgatgc
    14221 cggcccagaa cattccctgc gccagcgcga acaccagaac cagaatagcg cctgaacagg
    14281 gtcggatgcc gacggtgaga accgcgctga aaccgcgccg ccagccgccg ggaccgccga
    14341 gttgcgcggg ctcaggaccg tgggaatgcc cgcaatgctc gtcgtggaca tggccgtgat
    14401 cgtgatgctc cgcatgttcg tgttcaggca catgatcggg agcgacagtt gggtgcgcag
    14461 gggcgtgggc gtgatgatga tgtccgtggt cgccatggcc gtgcgagtga tgtccatgat
    14521 gacgctgaat tgccgcgtgc gccagagccg gcgcaagccg tggctcgaat ttgccgtggg
    14581 atgactgcag cgcccggatc aatccaccgc ccttggtcca gaccagccgg gagccgaaag
    14641 ctgcgatcag cgtgtagctg gcaatctcga tcacgcgctc ggcgccgcac atggtcttcg
    14701 cggtgacgtt gagcagccag gcgccgaggc cgacgatcag caccgcgatc aacgactgca
    14761 tcagcgccga gacaaacgac agcacgatac cgcgtcgtgc ggtctcttca ttggcgacaa
    14821 ggtatgacga aatcaccgtc ttgccgtggc cagggcccgc cgcgtgaaac atgccgtagc
    14881 caaacgaaat cgcaagcagc gtccagaccg cgctgccgtc ggccttggcg gcgcgaatgg
    14941 tcgatgacat ctcgcggtag aattcggatt gcttggtcag cagccagccg acgatacccc
    15001 ctacttgcgc gtcgggagcg gcaggtgggc cgccgaacgg attttgcgcc agcgcgctct
    15061 gcagggcgcc atcgacgacc atcgtggccg ccatgatcgc aaggcagctc aacgcaatgt
    15121 gtcggtccgt tttcatgcgc gtcatcctca tggacaatcc acggtgatct tgttggcgaa
    15181 catcacgccg tagttaccgt tgctgccatt gagaaaggaa gactcatcca gcttctgggc
    15241 gccggccgct ccgtcgttcg gccgctggat ctccatggag caagaggcgg gtgccccgac
    15301 aaggcggatt ggatcctgct tctgcagcgc gaaatccacg aaataggttg gatcgaagat
    15361 ctcgaccgcg agctgcctgg tcttgatcgg cgtcttcagc gggagcgtaa agtggagcac
    15421 caaagcggca tttttgtatt caagaaaata gtcggtcggg tccagaaatg gttgtttttt
    15481 gccgtcgacc ttggcgaccg tgaagaagcc gtattccttc agggattcca cattggtctg
    15541 cgccagcggc gccagttctt cgcgggtata gatccctttc cttttggtcg cgacgccttg
    15601 caatgcgtag gtcgagaaca tgtcgtcgaa ggcccaggcg tgacggacgc tggtgatgga
    15661 gccatccggc gcatagatca gttcagtcgc cgcggtaacc caaacgtgcg gatgggccgc
    15721 cgccgggctt accgaattgg acagcgccac agcggcgaca aacgccagca actggattga
    15781 ccaggtcact ggcggagcct tggtcttttg gaagcgatcc atcaaactcc tccaccgatc
    15841 ctcaaagcta cgagcaaggg cttcgatctg ccgtgacaga tgcgcgatca gctactttgc
    15901 cgcgcgcgaa ggcgcgctgc cgggagaact cacctgggcg cgatcgagat cgcgcgacag
    15961 caatcgcccg gcggcgcccg acgaccggct gaccagttcg aggtagcctt tggtcacgcg
    16021 ggaaaaatga ttgtagatga cgacggccgg gatcgccgcg accaacccca tcgcggtggc
    16081 gagcagcgct tcggcgatac cgggtgctac cacggccaga ttggtggtct gcgattttga
    16141 gatgccgata aagctgttca tgatgcccca caccgtgccg aacagcccga cgaacggcga
    16201 ggtcgcgccg atcgtcgcca gcactcccat tccgagccgg atccggcgcg cttcggcgcg
    16261 cacgatctcg gcaaagctcg atgccgcccg ttccttgatg ccgctgtcgc tggaaattcc
    16321 cgccgacagc cgcgcctcgc gcatcgccgc cgacagcaat ctcgacaata cgctgcccct
    16381 ggcgccgagc gcgaattgcg cctccgccat cgaccgtgcg tcactgattt tgccgagggc
    16441 tgcgcgcagc tggcgctggg ccagcgacag ctcgatcatc ttggcgagga agacggtcca
    16501 tgtcaccagc gaggcgaacg ccaggccgat catcacggcc ttgaccagga tgtccgccga
    16561 caggaacatc gaccagggcg acaattcgcg caacgccacc gtcgtcgatt tcagcgaccg
    16621 gccgccatcg accggagcag ccgatacgtc gccagccgcg ggcgtggact gaccggccgc
    16681 cgatggcgcc ggagcctgcg actccggcgc ggcctgcgcg ggcggcgttt gcgagagcgc
    16741 cggagtttgc ggcgtctgct gctgtgcgag cgacggcagt gccgtcgaaa aaatcgccag
    16801 cgcgacaatc ccgctataaa cagcccgtga aagcgacttg ttcatgcctt ggcccagcag
    16861 cggatcgtat tgtgatagat acatccgctg attcagatct tctgcgagat gccgacgaag
    16921 aagccgcgtc gtggtccgaa ctgcggtgcg aacacgccga tgccggagcc gtcccggatc
    16981 tcgtagatct tgtcgaacac gttcaccaca tcgaaccgca gcgtggtcgg cttatttggc
    17041 gccacgataa agaaatcgtg cgatacgccc atattgacct gggtgtagga cggcaggtga
    17101 tcagtgttga cgaagcccga acgaagcccg ctgccataga tcatcgaagc ggaaaagcgc
    17161 gtatctttcc acaagtagga agccccggcc gatgcggtca agacctggct gtggtcggtg
    17221 tagatgtaat ggttggcaat gtaggccagt tcgtccgcgc cgaacaggaa ctggttggag
    17281 acgacattgg tgccgagctg gcgtgcccaa gcaacgttgc cgtacatccg aagattgccc
    17341 attgcataga gcgccttgaa ttcgaggccg atattgttcg ccttggcata gttgaatcca
    17401 tcgaggacat aggcttggcc gaattgtccg tcatccagca gatcgcgcgc gagcttgaga
    17461 tagccatcaa ggccgacttc gagacccgga accggcca
//
LOCUS       DATNOI010000481         2607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_387235,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000481 DATNOI010000000
VERSION     DATNOI010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387235"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VN968_19275"
     CDS             <1..322
                     /locus_tag="VN968_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31412.1"
                     /translation="LATEPKVLLLDEAMAGLTQREVQLAIDLVRDIHRSGITLVIVEH
                     IMEVIMSLATRVVVFHQGREIARGSPREVTGNPAVIAAYLGTRAAKAAAGHTPIELMG
                     GPAT"
     gene            319..1038
                     /locus_tag="VN968_19280"
     CDS             319..1038
                     /locus_tag="VN968_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31413.1"
                     /translation="MTAPLLKVEHLEVRYGDLIGVSDVSLDVPEGSVVALLGSNGGGK
                     TTTLNAVAGLIPVHSGSIGFRGQEIGGQTAFSIVRKGLALSPEGWRLFVQQSVENNLL
                     LGATPLRDRALAAVLLDRVYEIFPKLAERRSQRAGTMSGGERQMLAVGRALMSDPKLL
                     MLDEPSLGLAPAVVESMYETFGRLHREGLTILLAEQSIELALEVSDFATVLQVGKSVL
                     TGTAAELANDPQVQRAYLGTG"
     gene            1157..1687
                     /locus_tag="VN968_19285"
     CDS             1157..1687
                     /locus_tag="VN968_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31414.1"
                     /translation="MRRWISAALLLTSAAAANAQTPYAGMQARPIKALSEQQVADLGA
                     GRGMGLALAAELNGYPGPSHLLELADKLNLSTDQRAAIQRLFDSMKAEAVPLGSKLIE
                     QEADLDTQFASHTVTPESLKASTAAVAATQGALRETHLKYHLSTAALLSSGQMQRYAE
                     LRGYGGQAAPMQHHHH"
     gene            complement(1748..1852)
                     /locus_tag="VN968_19290"
                     /pseudo
     CDS             complement(1748..1852)
                     /locus_tag="VN968_19290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(1866..2402)
                     /locus_tag="VN968_19295"
     CDS             complement(1866..2402)
                     /locus_tag="VN968_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HXL31415.1"
                     /translation="MSIKLAVVAIVAFGSTGLGHAQQATKSKSAPPPAETPSANAPPP
                     PGWAARCTSASRNAPPECAMEQTAVLTKTGQLIVLVNIRVPNDTRTPVALVQLPLGLN
                     LPGGAKFQIDDGKPTDLQIQTCEARGCYAGTPIAPELLAVLKSGKQLKVSFQNLGKET
                     ITIPMPLTDFAAAYDKIK"
BASE COUNT          477 a          791 c          851 g          488 t
ORIGIN      
        1 gctcgccacc gagcccaagg ttctgctgct cgatgaagcg atggcgggcc tcacccagcg
       61 cgaggtgcaa ctcgcgatcg atctcgtgcg cgacatccat cgctcgggca tcaccctggt
      121 cattgtcgag cacatcatgg aagtcatcat gtcgctggcg acccgggtgg tggtgtttca
      181 ccagggcagg gagatcgcgc gcggcagtcc gcgcgaggtc acgggcaacc cggccgtgat
      241 cgcggcctat ctcggtacgc gcgccgccaa ggccgccgct ggccacacgc cgatcgagtt
      301 gatgggcgga cccgcgacat gaccgcgcct ttgctcaagg tcgagcatct ggaagttcgc
      361 tatggcgacc tgatcggcgt atccgatgtt tcactagatg tgccggaggg cagcgtcgtc
      421 gcgctgctcg gttccaatgg cggcggcaag accaccacgc tcaacgccgt cgccggtctc
      481 atcccggtgc attccggatc gatcgggttt cgcggccagg agatcggcgg tcaaaccgcg
      541 ttttcgatcg tgcgcaaagg cctcgctctg tcgccggaag gctggcggct gttcgtgcag
      601 caaagcgtcg aaaacaatct gcttttgggc gccacgccgc tgcgcgacag ggcgctcgcc
      661 gcggtattgc tcgatcgggt ctacgaaatc tttccaaaat tggcggagcg gcgcagccag
      721 cgggcgggca ccatgtccgg cggcgagcgg cagatgctgg cggtcggccg tgccctgatg
      781 agcgatccca aactgctgat gctcgatgag ccgtcgctcg gcctggcgcc tgcggtggtc
      841 gaatcgatgt acgagacctt cggccggctg caccgggaag ggctgaccat cctgctcgcg
      901 gagcaatcca ttgagctcgc gctcgaggtt tcggatttcg ccaccgtgct gcaggtcggc
      961 aagagcgttc tgaccggcac cgcggcagaa ctcgccaacg atccgcaggt gcagcgcgcc
     1021 tatctcggca ccggctgata ttggcgcggc ccgtttggcg gtatcatgcg tgacaaaccg
     1081 ggacggcgcc ctggaaggag ccagggaaaa ttgcgaagga tagacctgag gcaaagtaaa
     1141 caaacggagc gagaacatgc gacgatggat tagcgccgct ttacttctga catctgccgc
     1201 ggcagccaac gcccagacgc cttacgccgg aatgcaggcg cgaccgatca aggcattgtc
     1261 cgaacagcag gtggcggacc ttggcgccgg tcgcggcatg gggctggcgc tcgccgccga
     1321 attgaacggt taccccgggc cgtcccatct cctggaactg gctgacaaac ttaacctgtc
     1381 gacggatcag cgcgccgcca ttcagcggct gttcgattcc atgaaggccg aagccgtacc
     1441 gctgggctcc aaattgatcg aacaggaagc cgatctggac acgcaattcg ccagccacac
     1501 cgtgacgccg gaaagtctga aggcgtcaac cgcggcggtc gccgcgacgc aaggcgctct
     1561 tcgcgaaacc catctcaaat atcatctgtc gacggctgcg cttctgagct ccggccagat
     1621 gcagcgctat gccgagttgc gcggctatgg cggtcaggcc gcaccgatgc agcatcacca
     1681 tcactgatgc cggccatttc ctgcggctga cggaattgcg aacggaggcc ttggacgacg
     1741 ttgccttcat ctcggcctcg agcagctgca acaggcagct gcttaatcgg acttccatct
     1801 cacggtcgtg aagggatagc ggcccgggat cgttcaggat ggcgttgacc agcgcggcag
     1861 gaaagctatt ttatcttgtc gtaagcggcg gcgaaatcgg tcagcggcat tggaatggtg
     1921 atcgtttcct tgccgagatt ctggaacgat accttcaact gctttcccga tttcaaaacg
     1981 gccagcagct cgggcgcgat cggtgtcccg gcatagcagc cccgagcctc gcaggtctgg
     2041 atctgcaggt cggttggctt gccgtcatcg atctggaatt tggcgccacc tggcagatta
     2101 agtccgagcg gcaattgcac cagggcgacc ggcgtacggg tgtcgttggg cacgcgaatg
     2161 ttgacgagca cgatcagttg tccggtcttg gtgaggacgg cggtctgctc catcgcgcat
     2221 tcgggcggcg cattgcggct cgcgctggtg catcgtgcgg cccagccggg cggcggcgga
     2281 gcgttagcgg acggggtttc ggccggaggc ggcgcgcttt tggatttggt tgcctgctgg
     2341 gcgtggccga gcccggtcga tccgaaggcg acgatagcca cgactgcaag ctttatggaa
     2401 attttcactg atcacgctcc gagatgaata cgccttcgga tgtgccggag cgggctgaaa
     2461 gtcaagtcac tcggcggctt gcttgatcga gcctcgctca ctcgcgggcc gttcgtgatc
     2521 ggcatcctcc gatcgaccta gaccggaaaa tgcgttcgag agccggtcga gatagagata
     2581 caccaccggc gtcgtgaaca gcgtcag
//
LOCUS       DATNOI010000482         3897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1484234,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000482 DATNOI010000000
VERSION     DATNOI010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484234"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VN968_19300"
     CDS             <1..231
                     /locus_tag="VN968_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXL31416.1"
                     /translation="SRILPDHTHVTELRIEKDKLQIVGITQDAPSLVKLIEQSPHFTH
                     ATFFAPTTRSAGDPGERFHVEAGIKPYFGLGT"
     gene            228..788
                     /gene="gspM"
                     /locus_tag="VN968_19305"
     CDS             228..788
                     /gene="gspM"
                     /locus_tag="VN968_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HXL31417.1"
                     /translation="MNLAKTFATGASAPRYVAASLYGCLLLVLMAATTLPLKGVLDQR
                     AAVESQSDMLLRLEARGPATAHLEAAGDDTMVGSAFLEGSTVTIAGAALLQRVVGAVT
                     RHGGSVSSSQLDLQSTQAKDGFISVTASFDLEQQALQQIIYDLEAGMPFLFVDQLAVQ
                     GPAPSMTSGEKLRVLISVSGQWQGRQ"
     gene            785..1501
                     /locus_tag="VN968_19310"
     CDS             785..1501
                     /locus_tag="VN968_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31418.1"
                     /translation="MSSLGRVCVVGCFWLVVTASALGVAHPAGIDMSDTDAAAAARPR
                     PSPPAQDQRVAQTGQPPSGNPLWGIPLKQLSATRDRPIFSPSRRPPPAAVPVTAAATS
                     LPVQAPKQQERPQLSLIGTIVSGDDGFAIFVDQTTKAPLRIRLGANYQGWMLRQIEAR
                     SVTLQKGEEVVMLSFPEPANGQHTPAGADFAKTWPDPPAPLPPLASTAQTTPPFLSPE
                     SNALRIPARPHGGSARRRQM"
     gene            complement(1562..3373)
                     /locus_tag="VN968_19315"
     CDS             complement(1562..3373)
                     /locus_tag="VN968_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXL31419.1"
                     /translation="MIRTLVATLLLVAVASSGSQAQSDTDPARPDPGQRTSAAAGPVV
                     VLPSQRLPHPSSLRAKASGPIVVLQNATPEMNAPANSNRDLVDDRTRQPRQTPSAQPG
                     AAEAMPANLEGNRNMAAGFREPAASDALYGATHFRSTAPRREPDGFFGAPNAGMLTAG
                     CGVRRDGRQPPNMEDKCMQALAFAVSPPPATREVLPRNLPRIPPRDAQPIPPVAADKP
                     MTPPAKQMDPYEPTGFTAGNFVIKPAVEMTTGYDTNPARIPGGTGSPFVVVAPVVSVR
                     SQFDRHLLNADVRAAYIDDTQLQRLSHPTVDARVNGRYDFTDTTALNGEGHFMLDGDD
                     PGTARFTGRFARIPLVSTTGGSVGVTQKVDPLEVSVKGAVDHIQFQNVLLTDGQILSN
                     QDRNFNQYAVQSRASYALTNEFHPFVDVAVDRRVHELPVDMLGFRRDSTGTAVEGGVT
                     FAITDKLTGDAAIGYLVRQYQDMMLRPVSGVIADANLMYQFTKETTLQFGAKSQVAEI
                     AEAGISGVFKRDVTVELDHQFQPWLVGTLMTGYGADVFVGSPRVDNRYFVDIGMLYKV
                     SRLLQLKANLRQEATRSNIVQNNLNATVVSVGARVQY"
BASE COUNT          708 a         1228 c         1220 g          741 t
ORIGIN      
        1 tcgcggattc ttcccgacca tacccatgtg accgaactgc ggatcgagaa agacaagctg
       61 cagattgtcg ggatcaccca ggacgcgcct tcgctggtca aattgatcga gcaatctccg
      121 cacttcaccc acgcgacctt ctttgcgccc acaacacgtt cggctggcga ccccggcgaa
      181 cggtttcacg tcgaagcggg catcaaacct tattttggtc tcggcacatg aatttggcaa
      241 aaacctttgc tactggggcc agtgccccgc gctatgtggc ggcctcgctc tatgggtgcc
      301 tgctgctggt gctcatggct gcgaccactc tgccgttgaa gggcgtgctc gatcagcggg
      361 cggcggtgga atcgcaaagc gatatgctgc ttcggctgga agcccgcggc ccggcgacgg
      421 cgcatctcga agctgccggt gacgacacca tggtggggtc ggcctttctc gagggatcca
      481 cggtgacgat cgccggcgca gccttgctgc aacgtgtagt cggggcggtg acccgtcatg
      541 gcggaagtgt ttcgtcttcc cagctcgact tgcaaagcac tcaagccaag gacggcttca
      601 tcagcgtgac agcgagtttc gatctggagc agcaggcact gcagcagatc atttacgacc
      661 tcgaggccgg aatgccgttt ctgttcgtcg accaactggc ggtacaaggg ccggcgcctt
      721 cgatgacttc cggcgaaaag ctacgcgtgc tgatttcggt ttctggacag tggcagggtc
      781 ggcaatgagc agcctcggca gggtttgtgt ggtcggttgt ttctggcttg tcgtaaccgc
      841 ttcggcattg ggcgtcgccc atccggcggg tatcgacatg tcggataccg acgccgcggc
      901 agccgcgagg ccgcggcctt cgccgccggc gcaagaccaa cgcgtcgcgc agaccggaca
      961 accgccgagc ggcaacccgc tgtgggggat tccgctcaag caactgtcgg cgacgcggga
     1021 tcgtccgatt ttttcgccgt cacgccgccc tcctccggcg gccgtgccgg tcacggccgc
     1081 ggctactagt ctcccggtgc aagccccgaa acaacaggaa cgcccacaac tttccctgat
     1141 cgggaccatt gtgagcggcg atgacggttt cgcgatcttc gtcgatcaga ccaccaaagc
     1201 cccgttgcgg atcaggctag gcgcgaacta tcagggctgg atgctgcgtc agatcgaagc
     1261 gagatcggtg accctgcaaa agggcgagga ggtcgtgatg ctctccttcc ccgagccggc
     1321 aaacggtcag cacaccccgg ccggtgcgga cttcgcgaag acttggcccg atcccccagc
     1381 gcctctgcca ccgctggctt ccacggctca aacaacgccg ccttttctgt cgcctgaatc
     1441 caacgcactt cggataccgg ctcggccaca tggcggttct gcccggcgcc gccagatgta
     1501 gcctttcagg agattgtcga ttcggtccga cggaggaagc tgaattcatt ctcgctgcgc
     1561 gtcaatattg cacacgcgcg ccgaccgata ccacggtcgc gttgagattg ttctgcacga
     1621 tgttcgatcg cgtcgcctcc tgccgcagat tggccttgag ctgaagcagc cgcgacacct
     1681 tgtacaacat gccgatatcg acgaaatagc ggttgtccac ccgcggcgag ccgacgaaca
     1741 cgtccgcgcc ataaccggtc atcagcgtac ccaccagcca gggctggaac tggtgatcga
     1801 gttcgacggt gacatcgcgc ttgaacacgc cggagatgcc ggcctcggcg atctccgcca
     1861 cttgcgactt tgcgccgaac tgaagcgtgg tctccttggt aaactggtac atcaggttgg
     1921 catcagcgat gacgccgctg accggccgca gcatcatgtc ctgatactgt cgtaccagat
     1981 agccgatcgc ggcgtcgcca gtcagcttgt cggttatcgc gaaggtgacg ccaccttcga
     2041 ccgccgttcc ggtcgaatcg cgacggaagc ccagcatatc caccggcagc tcgtgcacgc
     2101 gccggtcgac cgcgacatcg acgaagggat gaaattcgtt ggtcaatgca taactggcgc
     2161 gactctgaac cgcgtactgg ttgaaattgc ggtcctggtt gctgaggatc tggccgtcgg
     2221 tcagcaggac gttctggaat tggatgtgat cgaccgctcc cttgaccgac acctccaggg
     2281 gatcgacttt ctgggtgacg ccgacgctgc cgcccgtggt cgaaaccagc ggaatcctgg
     2341 cgaagcggcc ggtgaatcgc gcggtgccgg gatcgtcgcc atcgagcatg aagtggccct
     2401 cgccattcag cgcggtggtg tcggtaaagt catagcggcc attgacccgg gcatcgacgg
     2461 tcggatggct cagtcgctga agctgggtgt cgtcgatata ggctgcgcgc acgtcggcgt
     2521 tgagcagatg gcgatcgaac tgggagcgca ccgacaccac aggtgcgacg accacgaacg
     2581 gtgaaccggt cccgcccggg attcgcgcgg gattggtgtc atagcccgtt gtcatttcga
     2641 cagccggctt tatcacgaaa ttgccggccg tgaagccggt cggctcatac gggtccatct
     2701 gctttgccgg cggcgtcatg ggcttgtcgg cggcgaccgg cggaatcggc tgcgcgtccc
     2761 taggcggaat ccgcggcagg tttcgcggca gcacctcccg ggtggcagga ggcgggctca
     2821 ccgcgaaggc aagcgcctgc atacatttgt cctccatgtt cggcggctgg cgcccgtcgc
     2881 gcctgacgcc gcagccggca gtcagcatgc cggcgtttgg tgctccgaaa aacccgtcgg
     2941 gctcgcggcg cggagcggta ctgcgaaaat gcgtggcgcc gtacagcgca tccgacgcgg
     3001 cgggttcacg aaagccagcc gccatgttcc ggttgccttc gagattggcc ggcatcgcct
     3061 cggcagcacc cggctgcgcc gatggagttt gccgaggttg cctcgtgcgg tcatcgacca
     3121 ggtcacggtt actgttagcc ggtgcattca tctccggagt tgcattttgt agaacgacga
     3181 tcggaccgct ggccttcgca cgcagagaac tcgggtgagg caggcgctgc gaaggcagca
     3241 cgacgaccgg tccggctgcg gcggatgtcc tctggccagg atcggggcgc gccggatctg
     3301 tatcgctctg cgcctgactt cccgacgacg caaccgcaac cagcaacagc gtcgcgacga
     3361 gcgtccgaat catacgggcg gcgcgatgta ccaaacgatc gcaaccgttc cccgcacgcc
     3421 ccgtcatgtc ccccagtctc cgtgcgtcct gctcgacgca gaactaacgt gacaccaaca
     3481 cagcggcaga agtcggagcg agttgtgatc tcccgaactg aatgacggca acttgttgat
     3541 tcattgcggc acggttgcag gattcggaaa agcaacctgg ctccgcgacg aggcgtgacc
     3601 ttcaaatcgt tgcttggaag gctcttggcg gccacgcatt gatcgggatg tcgacttgtt
     3661 ttgagcaccg tttcgatccg caacgggctc gccttgcacc cagggttgca ggcggcctcg
     3721 aacctcgccg atagcgaccg atgatcgttc acggaaacca agcgtgccgt gccatcttcg
     3781 aagcgcaccg aagcgacgac aactcggaac gctgcggcaa tcgcgacgcg atcgccgccc
     3841 tgcccgttga tgccgttagc ggctttcctg tggaggtgaa gtcgacgcca tcgacgc
//
LOCUS       DATNOI010000483         5159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2579458,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000483 DATNOI010000000
VERSION     DATNOI010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2579458"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VN968_19320"
     CDS             <1..1333
                     /locus_tag="VN968_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXL31420.1"
                     /translation="PRPAAVNVPNDLAAHWMPFTANRAFKKAPRLLAGAKDMHYITVD
                     GRKIIDAAAGMWCCNAGHGRTQISAAIARQAEALDYAPPFQFGIPQAFELASRIAELA
                     PAGLDHVFFCNSGSEAADTALKIALAYHQLNGQGTRSRLIGRERGYHGVGFGGTAVGG
                     IVNNRKMFGTLLAGVDHLPHTYDREKQAFTKGEPEYGAHFADELERLVNLHGASTIAA
                     VIVEPMAGSTGVLPAPKGYLKRLREITQKHGILLIFDEVITGYGRLGFAFAAERYGVL
                     PDMLTFAKGITNGAAPMGGVLVRDTIHDAFMSGPEHIVELTHGYTYSAHPLACAAALA
                     TLDIYRDEKLFERANKLESKFADAVMSLKGQPNVVDIRTVGLTAGIDLAPRPDAPGKR
                     GFDGLNSAFHDNDLMLRVAGDTLALTPPLIVTEDQIGEIIEKVTKVIRAVA"
     gene            1620..2213
                     /locus_tag="VN968_19325"
     CDS             1620..2213
                     /locus_tag="VN968_19325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXL31421.1"
                     /translation="MLAPSSSLLRTGLALKLSQIRLATRSYLRDRTNQATGTVASYAV
                     AAGLFAAAGIFLVAACLVGITALFRWTEINYGPFWAFGAVAALLLVIAAICAAVAASQ
                     LKRPPPHFPSLTSRLRVAIKAGPLKSGQIEPLRDAAAANLTSPSAAATRANRRRRKAN
                     HGPGRNNRNIGAGLILTATLLGWAAARRRQQARRADV"
     gene            2213..3349
                     /locus_tag="VN968_19330"
     CDS             2213..3349
                     /locus_tag="VN968_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXL31422.1"
                     /translation="MPGRRSLPSDNWLWIAATTILILTAQRHFQAKAPRKPAPQSANP
                     AVAGRGNLAREVSQPGHGRPSSNPLQIPWAGWKDILWRTYMRSGEDRLLAIAAGVVFF
                     GLLAVFPAVTALVSCYGLFANPSTIGGNLQTLALMLPEGSFQIVQDQIARVVDKGGTA
                     LGATFLFGLALAIWSANAGVKAVIDALNVVYEEREKRSFVRLNLLSLAFTSGGIVALL
                     LMVSAVVAFPLALDHVGLAPASQSIVSLLRWPLLFLMLLIALGVLYRFGPSRRAARWE
                     WLSAGTLAAALLWIAGSCLLSWYLSNFGNYDATYGSLGAAIGLMMWMWMSAIIVLFGA
                     ELNSEIEHQTAPDTTIGRPKPLGTRDAVMADTLGASVPAMSKEH"
     gene            3474..4925
                     /locus_tag="VN968_19335"
     CDS             3474..4925
                     /locus_tag="VN968_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXL31423.1"
                     /translation="MIRISTIFIAICMVLFAASLGLVLYSVAGISGTESAIVALTALT
                     FLILYNAVSMRLRDRTDVGGQIADLSRGTADLARQVAEFGRRLAAIEGRVVSANSAGA
                     DRIQAVVGEISELGVLVKQLAVSVAAHDDMLASGVPATAPAPATRPADAPPSEPPPSI
                     ANEKSVAMAAAAPVKIVEEPAPSRSPAQILAAVKSAVEENRIDIYLQPMVTLPQRKVR
                     FYEAVTRLRDDEDQILTADDFISIAEAAGLIGRIDHMVMLRCVQVLRRLMVRNKDVGI
                     FCNVAAATLGNPTTFTQCLDFLEANRALASSFVLEFKQTTFRNLGPTETEHLAALAQR
                     GYRFSIDHVSNLRIEPRELADRGVRFIKVPAALLLDQKLNSASDIHPSDLSDLLGRFG
                     IDLIAERIEGERAVVDLLDFDVRFGQGFLFAPPRPLRPEGASATGGAAPNKAQVSQVS
                     NGSDNIASVNGAAIDPPRATGNAALARRAAGPA"
     gene            4941..>5159
                     /locus_tag="VN968_19340"
     CDS             4941..>5159
                     /locus_tag="VN968_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HXL31424.1"
                     /translation="MTTLRFVEKLRDLVGGVDVVLSDIWGVVHNGLVAFPEACEALHS
                     FRAQGGTVILITNAPRPADSVQRQLRKLG"
BASE COUNT          891 a         1724 c         1624 g          920 t
ORIGIN      
        1 cccacgaccc gccgccgtca acgtacccaa cgacctcgcc gcgcactgga tgccgttcac
       61 ggccaaccgg gccttcaaaa aggcgccgcg gctgctcgct ggcgccaagg acatgcacta
      121 catcacggtc gacggccgca agatcatcga cgccgccgcg gggatgtggt gctgcaatgc
      181 cggccacggc cgcacccaga tctcggcggc gatcgccagg caggccgagg cactggacta
      241 tgcgccgccg tttcaattcg gcattccaca ggcctttgag ctcgccagcc gcatcgccga
      301 actcgcgccc gccggactcg atcacgtttt cttttgcaat tccggatcgg aagccgccga
      361 taccgcgctg aagatcgcgc tggcctatca tcagctcaac ggccagggca cccgcagccg
      421 cctgatcggc cgcgagcgcg gctatcacgg tgtcggcttc ggcggcaccg cggtcggagg
      481 catcgtcaac aaccgcaaga tgttcggaac gctgctggcc ggcgtcgatc atcttcccca
      541 tacctatgac cgcgaaaaac aggccttcac caagggcgag ccggaatatg gcgcgcattt
      601 cgccgacgaa ctggagcggc tggtcaacct gcatggcgcc tccaccattg ccgccgtcat
      661 cgtcgagccg atggccggat cgaccggcgt gctgccggcg ccgaagggct atctcaagcg
      721 tctgcgcgag atcacccaga agcacggcat tctcctgatt ttcgacgagg tcatcaccgg
      781 ttatggccgg ctcggttttg ccttcgccgc ggagcgttac ggcgtgctgc cggacatgct
      841 gacctttgcc aagggcatca ccaatggcgc agccccgatg ggcggcgtgc tggtgcgcga
      901 caccatccac gacgccttca tgagcgggcc ggagcacatc gtcgagctca cccacggcta
      961 cacttattct gcgcatccct tggcctgcgc cgcggccctt gccacgctcg atatttaccg
     1021 cgacgaaaag ctgttcgagc gcgccaacaa gctcgagtcc aaattcgccg acgccgtgat
     1081 gtcgctgaaa ggccagccca acgtggtgga catccggacc gtcgggctga cagccggcat
     1141 cgacctcgcg ccgcgcccgg acgcgccggg caagcgtggc ttcgacgggc tgaacagcgc
     1201 gttccacgac aacgacctga tgctgcgcgt tgccggcgat accctggcgc tgacgccgcc
     1261 gctgatcgtc accgaggacc agatcggcga gatcatcgag aaggtcacca aggtgattcg
     1321 cgccgtggcg tagcgacttc tcttagctct ccccgtccct tacggggaga ggtcgaaatt
     1381 cgagtaaaac tcgaatttcg ggtgaggggc aaggcattgc gcttgcctca tcgttagcat
     1441 tcgcaggccg gagagccctc accccaaccc tccaagagcg agcttcgctc gtctcgaatc
     1501 cgcaagcgcg gggcgaggga gcagacggcc gcgccccaga accggggaac cttcgaaacc
     1561 aatccacgtt caaccacggc agagacgatt agcgtctctg ccgcggaggg ttgacgaaaa
     1621 tgctcgcgcc atcgagcagc ttgctgcgca ccggactggc actcaagctc agccaaatca
     1681 ggttggcgac gcggtcgtat ctgcgcgacc ggaccaacca ggcgaccgga accgttgcat
     1741 cctatgccgt cgccgccggg ctgttcgcgg ccgcggggat ttttctggtc gcggcctgcc
     1801 ttgtcggcat caccgcgctg tttcgctgga ccgaaatcaa ttacgggccg ttttgggcgt
     1861 tcggcgccgt ggctgccttg ctgctggtga tcgcagcgat ctgcgccgcc gtggccgcca
     1921 gccaactcaa gcgtccgccg ccgcattttc cgagcctcac cagccgtctg cgcgtcgcca
     1981 tcaaggccgg cccgctcaag tcgggtcaga tcgagccgct gcgggatgcc gccgcggcga
     2041 atttgacgag cccctcggcg gcggcgacac gcgccaaccg caggcggcgg aaggcgaacc
     2101 atggtcccgg gcggaacaac cgtaacatcg gcgccggcct gatcctgacg gcgacattgc
     2161 tgggctgggc ggccgcgcga cgacggcagc aagcgcgccg agcggacgtc tgatgcccgg
     2221 ccggcgatcg ctgccatcgg acaattggct ttggatcgcg gcgacaacga tcctgatctt
     2281 gaccgcgcag cgccattttc aggccaaggc gccccgaaaa ccggcccccc agtcggcgaa
     2341 tccagcggtc gcgggtcgtg gcaatctcgc gcgcgaagta tcgcagcccg gacacggacg
     2401 gccctccagt aatcccttgc agattccgtg ggccggctgg aaagacattt tgtggcgcac
     2461 ctatatgcgg tcgggcgaag accgtctgct cgccatcgcc gcaggcgtgg tgttcttcgg
     2521 cctgcttgcg gtatttccgg cggttacggc gctggtgtcc tgctacggtc tgttcgccaa
     2581 tccctcgacc atcggcggca atctgcaaac gctggcgctg atgttgccgg aaggctcatt
     2641 ccagatcgtg caggaccaga tcgcgcgggt agtcgacaag ggcggtaccg cgctgggcgc
     2701 gacattcctg ttcggccttg cgctggcgat ctggagcgcc aatgccggag tcaaagcggt
     2761 gatcgacgcc ctcaacgtcg tctatgaaga acgggaaaag cgcagctttg tccgattgaa
     2821 cctgctctcg ctggcgttca cgtcaggcgg gattgtcgca ttgctgctga tggtcagtgc
     2881 ggtggtggcg tttcctttgg cgctggatca tgtcggcctc gcgccggcaa gccagtccat
     2941 cgtcagcctg ctgcgatggc cgctgttgtt tctgatgttg ctgatagcgc tcggcgtcct
     3001 ctaccggttc ggacccagcc ggcgggcggc gcggtgggaa tggctcagcg ccggcacact
     3061 ggcggccgcc ttgctctgga tcgccggctc ctgcctcctg tcctggtacc tgtcgaattt
     3121 cggaaattac gacgcaacct acggttcgct gggtgccgcc atcgggctga tgatgtggat
     3181 gtggatgtcg gcgatcatcg tgctgttcgg cgccgaactg aattcggaaa tcgaacacca
     3241 gaccgcgcca gacacaacca ttggccgtcc aaagccgctg ggcacccgcg acgccgtcat
     3301 ggccgacacg ttgggggctt cggttccggc catgtcaaaa gaacattagc attccgaatc
     3361 aacattaatg ctaaggttgc ggcgcttggg ggaagcgcaa caattcgtgc gaacagcttc
     3421 cgcgtttctc gcaagcccgg atgaccaagg gacttgaggg ccgcgcgcgc aacatgatcc
     3481 gcatttccac gattttcatc gccatatgca tggtgctgtt cgccgcgtcg ctcggtttgg
     3541 tcctgtattc cgtggcgggc atcagtggaa cggaatccgc gatcgtggcg ctcaccgcgc
     3601 tgaccttcct gattctctac aacgccgtgt cgatgcggct gcgggaccgc accgacgtcg
     3661 gcggccagat cgcggattta tcgcgtggca ccgccgacct cgcgcgtcag gtcgccgaat
     3721 tcgggcgccg gctcgccgcc atcgagggcc gggtggtgtc ggccaattcg gccggcgccg
     3781 accgcattca ggcggtggtc ggtgaaatca gcgaactggg cgtgctggtc aaacagctcg
     3841 cggtctccgt cgcggcccat gacgacatgc tggcttcggg cgtgccggcc acagcacccg
     3901 cgccagccac caggccagct gatgccccac cgagcgagcc gccgccgtcc atcgccaacg
     3961 agaaaagcgt ggcgatggcc gcggcagcgc cggtgaaaat cgtcgaggag cccgctccct
     4021 cgcgcagccc ggcgcaaatc ctcgccgccg tgaaaagcgc ggtggaagaa aaccggatcg
     4081 acatctacct gcagccgatg gtgaccttgc cgcagcgaaa agtgcgcttc tacgaggcgg
     4141 tgacgcggct gcgcgacgac gaggatcaga ttctcaccgc cgacgatttc atctcgatcg
     4201 cggaagctgc cggcctgatc ggacggatcg atcacatggt gatgctgcgc tgcgtgcagg
     4261 tgctgcgccg gctgatggta cgcaacaagg atgtcgggat attctgcaac gtcgccgcgg
     4321 cgacgctcgg caatccgacc accttcacgc agtgcctcga tttcctcgaa gccaatcgtg
     4381 cgctggcgtc gtcattcgtg ctcgaattca agcagaccac gttccgcaat ctgggcccga
     4441 ccgagaccga acatctggcg gcgctggccc agcgcggcta tcgtttctcc atcgatcacg
     4501 tcagcaacct gcggatcgag ccgcgcgaac tggccgaccg cggcgtccgc ttcatcaagg
     4561 tgccggctgc cctgctgctc gaccaaaagc tgaactcggc gtccgacatt catccatccg
     4621 acctgtccga tctgctcggg cgcttcggca tcgacctgat cgccgaacgg atcgaaggcg
     4681 aacgcgccgt ggtcgatctg ctcgacttcg atgtccgctt cgggcaggga ttcttgtttg
     4741 cgccgccccg gccgttgcga cccgaggggg catctgctac cggcggggct gctccgaaca
     4801 aggcgcaggt atcccaggta tccaatggct ccgacaacat cgcttccgtc aatggtgcgg
     4861 ccatcgatcc gccgcgcgcg accggcaacg ccgcgctggc gcggcgcgcc gccggcccgg
     4921 cctgaccggc aatccacgcg atgacgacgc tccggttcgt cgagaaactg cgcgacctcg
     4981 tcggcggcgt cgatgtcgtg ctcagcgata tctggggggt ggttcacaac gggctcgttg
     5041 catttccgga agcctgcgag gcgctgcaca gctttcgcgc ccagggcggc accgtgatcc
     5101 tcattaccaa cgcgccgcgg ccggccgatt cggtgcagcg acaattgcgc aagcttggc
//
LOCUS       DATNOI010000484         7099 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_901197,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000484 DATNOI010000000
VERSION     DATNOI010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901197"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1510)
                     /locus_tag="VN968_19345"
     CDS             complement(8..1510)
                     /locus_tag="VN968_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31425.1"
                     /translation="MFKKLIPFAAAAMTALFLLPQPVAAQGKKDGVTMAMALEPPGLD
                     PTSGAAAAIAEVTLYNLYETLTKVKEDGSAVPLLAESWQTAPDLKTYTFKLRKGVKFQ
                     NGEPLNAETVKFSFERSAAPTSTNKDKSLFQSIAEITTPDADTVVIKMKNPEPNLPFL
                     LGQAPASIVEPKSVATNGTNPVGTGPFTVAAWAKGSSITLNKWPEYRNAAAIKLNKVV
                     IRFISDPSAQVASLLSGDVDAFPRVAAARSLAQFKADPRFVVMIGGSRSKTIVAINNK
                     KKPFDDVRVRRAVLAAIDRKAMIEGAVDGFGVPIGSFYTPGSLGYIDTTDINPFDIEK
                     AKKLLAEAGVTTPLEVSLKLPPPAYARQGGEVLAAQLAKVGITAKIENLEWAQWLSGV
                     FNATGPQNYDLTIVSHVEPFDLVKYTEDNYYTGYRSDAFNALYKKITEAANEADRARL
                     LGDAQRVLATDAAAGFLFQPQLITIANKKLKGVWSQVPLYANDFSDWAWE"
     gene            complement(1576..2616)
                     /locus_tag="VN968_19350"
     CDS             complement(1576..2616)
                     /locus_tag="VN968_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HXL31426.1"
                     /translation="MSTMANAAAARVGPEPLLLEVDDLVQRYTLPRENLFQPAPQVLA
                     LNGVSVQLTAGKSLGVVGESGSGKSTFARTVMALETPTSGSVRLLGRDLHRLPADELR
                     RARCDFQMVFQDPYGSLDPRQTVARIVAEPLTAFGRVDRAEQRGRVAAVLKQVGLRDA
                     DMDKYPHEFSGGQRQRIAIARALITQPKLIVADEPVSALDVSVQAQVLNLLEDLQDEF
                     GLSYVLISHDLAVVEYLCDEIAVIYLGRIVEQGRPEDLFNRCAHPYTRALLEAVPRAR
                     PGRERQRRGTQVIASQSRGSTGCVYAGRCPYADAHCRAVAPVARSVGSGHYSACHHAE
                     TIMALPALPGEV"
     gene            complement(2613..3620)
                     /locus_tag="VN968_19355"
     CDS             complement(2613..3620)
                     /locus_tag="VN968_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31427.1"
                     /translation="MTDRKPLIEVDNLRVRLNTSRGPADAVRGVSFSLARGETLGLIG
                     ESGCGKSVTAMALMGLLPDSAVVSGNVRLEGQELVGLAESDYCKLRGNRVSMIFQEPM
                     TALNPMHTVGRQVAEPLRRHRAFSAAQARREAIALLDRVGLPDAAKRVDAYPHQFSGG
                     QRQRITIAMALACEPDLLIADEPTTALDVTIQGQILDLIADLVAERGMSMILISHDLG
                     VIAENVQRMMVMYGGTVVENGATEALFAGLGHPYSQGLFRARPRLGAPKGTRLQTIAG
                     IVPELADLPSGCAFADRCDIAEERCRATFPAPVTVNAGHTVRCLRTDVSMANRIGAVS
                     G"
     gene            complement(3620..4414)
                     /locus_tag="VN968_19360"
     CDS             complement(3620..4414)
                     /locus_tag="VN968_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31428.1"
                     /translation="MTGGVLTLFVISAALLSTIWTPWPAYEIDVAHKLNPPSATHWLG
                     TDVLGRDIVSLLLVGARSSIVVGVIAVGIGLLVGASLGLIAAARKGWVEELIMRLGDF
                     TFAFPALLSAIMLAAVAGAGMVTSIAAIGIFQIPVFLRITRGSANAIWAREFVLAARA
                     AGKGKFRITLEHVLPNILSILIVQATIQFALAILAEAALSYLGLGTQPPQPSWGRMLN
                     DAQSLLFQSPSLAVYPGLAIATAVLGLNLLGDGLRDLFDPRLSRER"
     gene            complement(4537..5487)
                     /locus_tag="VN968_19365"
     CDS             complement(4537..5487)
                     /locus_tag="VN968_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31429.1"
                     /translation="MTVFILRRFLTLIATLAGASLIIFLVLDALPGNAAEMLMGADSS
                     PEAIRAMTIKLGLDQPLVVRYLRWIGGLLIGDMGNSYSYGTPVAELIAERLVLTIPLA
                     VMAMLLTVVLALAAGVYTAANHNKIGDVGVMAMTQFGIAIPNFWFAILLILLFSVKLQ
                     WLSAGGFAGWGEGIVSGVRSLLLPAISLAVVQAAILARVTRSAVLEVLREDFVRTARA
                     KGLTRRQVLWGHVLRNAMIPVMTVMGLQFANLLAGTIVVENVFYLPGLGRLIFQSISN
                     RDLIVVRNCVMLLAAMVVIVNFVVDVLYAAIDPRIKASEL"
     gene            complement(5613..7061)
                     /locus_tag="VN968_19370"
     CDS             complement(5613..7061)
                     /locus_tag="VN968_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXL31430.1"
                     /translation="MSDELCFLSAADLRRRISRKEISPSEVTSAVIARAKRLQPELNC
                     FITLCADKALAQAKDAERRIMAGEPLRILEGIPFTVKDIVNTKDVRTTFGAIPYRDNV
                     PDHDAVAVARLRAAGAILIGKTTTPEFGTKCLTDSPLFGRTRNAWSGGRSSGGSSGGA
                     AVAVASGIAPLAVATDGGGSTRIPAACNGVVGLKQSNGVIPHSQVPDAFGNQTYVTPT
                     TRTVADTALMMQVMAGEDASDPWSIGIPARDFIGDALPVGDLSGKRILFCLAPPGRKA
                     SADVAAAFSASLKTLEALGAELEEMPGDGFDVEPIWRAINHTVWRTRFEKLAAEHRDE
                     LSDTFLKQLALAQRISGVDYQQAMFDRTILFRRVQALLQRADLLVMPTILRTALPIDQ
                     DLFGTVEIDGEVFDNVRPHWFPWTMPFNMTGHPAVSMPCGFGADGLPIGLQLVGQFRG
                     DAELLRACAVFELAQNSLDRWPNTVLLSSSMV"
BASE COUNT         1401 a         2278 c         2202 g         1218 t
ORIGIN      
        1 accaggctca ttcccaagcc caatccgaga aatcgttggc gtacagggga acctggctcc
       61 acacgccttt cagcttcttg ttcgcgatcg tgatcagttg cggctggaac aggaagccgg
      121 cggcggcatc ggtcgcgagc acgcgctggg cgtcgcccaa cagcctggca cgatcggcct
      181 cgtttgcggc ttccgtgatc ttcttgtaga gcgcgttgaa ggcgtccgac ctgtagccgg
      241 tgtaataatt gtcctcggtg tatttcacga ggtcgaacgg ctcaacatgg gagacaatgg
      301 tgagatcgta gttttgcgga ccggtggcgt tgaacacccc cgatagccat tgcgcccatt
      361 ccaaattctc gatcttggcg gtgataccga ccttggcaag ctgcgctgcc agcacctcac
      421 cgccctgccg cgcataagcc ggcggcggaa gcttcaggct gacttcgagc ggcgtggtca
      481 cgccggcctc ggcgagcagc ttctttgcct tctcgatatc gaacggattg atatccgtcg
      541 tatcgatgta gccaagcgag cccggcgtgt agaagctgcc gatcggcacg ccaaaaccgt
      601 cgaccgcacc ttcgatcatg gctttgcggt caatcgcggc gaggaccgcg cggcgaaccc
      661 gcacatcgtc gaacggcttc ttcttgttgt tgatggcgac gatggtcttg gaccgcgaac
      721 ctccgatcat gacgacgaat ctcgggtcgg ccttgaattg agcgaggctg cgcgcggcgg
      781 ccacgcgcgg gaacgcatcg acgtcgccag acagcagtga cgccacttgc gccgacgggt
      841 cgctgatgaa gcggatcacg actttgttga gcttgatcgc cgccgcattg cggtattccg
      901 gccatttgtt cagcgtgatc gacgaaccct tggcccaggc ggcaaccgtg aacggcccgg
      961 tgccaaccgg gttggtgccg ttggtagcca cgctcttcgg ctcgacgatc gaagcgggcg
     1021 cctgcccgag caggaacggc aggttcggct ccgggttctt catcttgatc acgacggtgt
     1081 cggcgtccgg cgtagtgatc tctgcgatgc tctggaacag gctcttatcc ttgttggtgc
     1141 tggttggcgc cgccgaccgc tcaaacgaga atttcacggt ctccgcgttc aacggttcgc
     1201 cgttctggaa cttcacgccc ttgcgcaact tgaaggtgta ggtcttcagg tccggtgccg
     1261 tttgccagct ttcggcaagc aatggcacgg ccgagccgtc ttccttcact ttggtgagcg
     1321 tctcatacaa attgtaaagc gtgacctcgg caatcgccgc ggcggccccg ctggtcgggt
     1381 cgagaccggg cggctccagc gccatcgcca tcgtcacgcc gtctttcttg ccctgcgcgg
     1441 caaccggttg cggcagcagg aaaagcgccg tcatcgctgc cgcggcgaaa ggaatgagct
     1501 ttttgaacat gtaaacgctc ctaaaacggg acgaatcgaa cgatacgtca aacagggatc
     1561 agcggcacca cactatcaca cctcgcccgg caaggcgggc agcgccatga tggtctcggc
     1621 gtgatgacag gcggaataat ggccggagcc aacgctgcgc gcgacaggcg cgaccgcgcg
     1681 gcaatgcgcg tcggcgtagg ggcatcgccc ggcatacacg cagcccgttg accccctcga
     1741 ttgcgacgcg atcacctggg tcccgcggcg ttgccgttct cggcctggac gggcgcgcgg
     1801 tacagcctcg agcaacgcac gggtataagg atgcgcacag cggttgaata aatcctccgg
     1861 ccttccctgc tcgacgatcc ggccgaggta aatcaccgca atctcgtcgc acagatattc
     1921 aaccaccgcg aggtcatgac tgatcaggac gtaactcaac ccaaattcgt cctgcaggtc
     1981 ttccagcaga ttgagcacct gcgcctgcac cgaaacatca agcgcgctga ccggctcatc
     2041 ggcgacgatc agtttgggct gcgtgatcag ggcgcgcgcg atggcgatac gctggcgctg
     2101 gccgccggag aattcgtgcg ggtacttgtc catatcggca tcgcgcagac ctacttgctt
     2161 cagcactgcc gcgacgcgac cgcgctgttc ggcacgatcg acacggccga acgcggtgag
     2221 cggttcggcg acgatgcgcg ccaccgtctg gcgtggatca agcgagccgt agggatcctg
     2281 gaacaccatc tggaagtcgc accttgcgcg gcgaagttca tcggcgggca aacgatgcag
     2341 atcgcgcccg agcaacctca cggaacccga tgttggcgtc tccagcgcca tcaccgtgcg
     2401 cgcaaaggtg gatttgccgg aaccggattc tccgaccacg ccgaggctct tgcccgccgt
     2461 cagttgcacg ctgacgccgt tgagcgccag cacctgcggg gcgggctgga acaggttttc
     2521 gcgcggcagc gtatagcgct gcacaaggtc atcgacttcg agaagcagcg gttcgggccc
     2581 aacgcgcgca gccgccgcat ttgccatcgt gctcatccgc tcaccgctcc aatccggttc
     2641 gccatcgaga cgtcggtacg caagcagcgc acggtgtggc cggcatttac ggtgacgggg
     2701 gctggaaacg tcgcccggca gcgttcctcg gcgatatcgc accggtcggc gaacgcgcaa
     2761 ccggaaggca ggtctgcgag ctccggcacg ataccggcga tggtttgcag gcgggtacct
     2821 ttgggcgcgc caagccgcgg ccgcgcgcgg aacaggcctt ggctgtaagg atgtcccagg
     2881 cctgcgaaca acgcttcggt cgcgccattt tctaccacgg tgccgccata catcaccatc
     2941 atgcgctgca cattttcggc gatgacgcca agatcgtggg aaatcaggat catcgacatg
     3001 ccgcgctcgg ccaccagatc ggcgatcagg tcgagaattt gcccctgaat ggtgacgtcg
     3061 agcgcagttg tcggctcgtc cgcgataagc agatccggct cacaggcgag cgccatggcg
     3121 atggtgatgc gttggcgctg gccgccggaa aactggtgtg ggtaggcatc gacccgcttt
     3181 gcggcatcgg gaaggccgac gcggtcgagc aatgcaatcg cttcccggcg cgcctgcgcg
     3241 gccgaaaaag ccctgtgcct gcgtagcggt tcggcaacct ggcggccgac cgtgtgcatc
     3301 ggattgagcg ccgtcatcgg ctcctgaaag atcatgctca cgcggttacc gcgcagcttg
     3361 cagtagtcgg attcggccaa gccgaccagc tcctgcccct ccagccgcac gttgccgctg
     3421 accacggcgc tgtccggcaa cagacccatc agcgccatcg cggtgaccga cttgccgcaa
     3481 ccggactctc cgatcagccc gagggtctcg ccgcgggcga gcgaaaagct gacgccgcgt
     3541 accgcgtcgg ctgggccacg gctggtattg agccggactc gcaaattgtc gacttcgatc
     3601 agcggctttc tgtcggtcat caccgctccc gcgaaagccg cggatcgaac aggtcccgca
     3661 gcccgtcgcc caagagattc agtcccagca ccgcggttgc gatcgcaaga cccggataga
     3721 cggcgagcga cggcgactga aacaagaggc tctgcgcgtc attgagcatc cggccccagg
     3781 atggctgcgg cggctgggtg ccgagaccga gataggagag cgccgcctcc gccaaaatcg
     3841 ccagcgcgaa ctggattgtc gcctgcacga tcaggatcga gagaatattg ggcagcacgt
     3901 gctcgagcgt gatgcggaat tttcccttgc cggccgcgcg cgccgccagc acgaactcgc
     3961 gtgcccagat cgcgttggcg gagccacggg taatgcgcag gaaaaccggg atctggaaga
     4021 tgccgatcgc cgcaatcgac gtgaccatac cagcccccgc aacagcggcc agcatgatcg
     4081 cggaaagcag cgccggaaac gcaaaagtga agtcgccgag ccgcatgatc agttcctcga
     4141 cccatccctt gcgggcggcc gcaatcagcc cgagcgaggc gccgaccagc agcccgatgc
     4201 ctaccgcaat gacgccgacc acaatcgagc tcctggcgcc gacgagcagc agcgaaacga
     4261 tatcgcgacc cagcacgtcg gtgccgagcc agtgcgtcgc agagggcgga ttgagcttgt
     4321 gcgccacgtc gatttcgtac gcgggccatg gcgtccagat cgtcgacagc agcgccgccg
     4381 agatcacgaa cagcgtgagc acgccgccgg tcatgaagct gcggtggcga agtgcgcgct
     4441 gcaaaaagcc cttgccggat cggcccggct ccgacgggac tgcggcctgc ggaggggcag
     4501 tgttcaacgg catcgctgca gcgggttgaa cgccactcat aattcgctag ccttgatccg
     4561 cggatcgatc gccgcataga gcacatcgac cacgaaattc acgatcacga ccatcgccgc
     4621 cagcaacatc acgcaattgc ggacaacgat gaggtcacgg ttcgagatcg actggaaaat
     4681 caagcgcccg aggccgggca aatagaacac gttctcgacc acgatggtcc ccgccagcaa
     4741 gttggcgaat tgcagcccca tcaccgtcat gaccgggatc atcgcgttgc gcaacacgtg
     4801 cccccacagt acctggcgcc gggtgaggcc cttggcccgc gccgtgcgaa caaaatcctc
     4861 gcgcaatacc tcgagcaccg ccgagcgcgt gacgcgcgcc aggatcgcgg cctgcaccac
     4921 cgcgagtgaa atcgccggaa gcagcagcga acggacgccg gaaacaatgc cctcacccca
     4981 tcccgcgaag ccgccggcag agagccactg cagcttcacc gagaacagca ggatgagcag
     5041 gatggcgaac cagaaattcg gaatcgcgat gccaaactgc gtcatggcca tcacaccgac
     5101 gtcaccgatc ttgttgtgat tggcggcggt atagactccg gcggcaagcg ccagcaccac
     5161 cgtcagaagc atcgccatga cggccagcgg tatggtcagg acgagccgct ccgcgatcag
     5221 ttcggccacc ggggtgccgt aggaataact attgcccata tcgccgatca gaagcccgcc
     5281 gatccagcgc agatagcgaa cgaccagcgg ctggtcgagc ccgagcttga tggtcatcgc
     5341 gcggattgct tcaggcgagg agtccgcccc catcagcatc tcggcggcat tacccggcaa
     5401 ggcatcgagc acgagaaaga tgatcagcga ggcgcccgcc agcgtcgcaa tcaatgtcag
     5461 aaagcgccgg agaatgaaga cggtcacgcg tcatttcctt tggcgcggaa tgcaccaggt
     5521 gggaggcatc gcatcgcgga tcatccgacc tttcggagca gcattcaata gaactccgcc
     5581 aatttgggcc tgccaccacc cacggccgac cgtcacacca tggacgacga cagaagaact
     5641 gtgttcggcc agcggtcgag agaattctgc gcaagttcga acaccgcaca agcacgcagc
     5701 aattcggcat cgccgcgaaa ttgtccgaca agctgcaggc cgatcggcag cccgtcggca
     5761 ccgaagccgc agggcatgct caccgccgga tgtccggtca tattgaaggg catcgtccag
     5821 ggaaaccagt gcgggcggac gttgtcgaac acctcaccgt cgatttcgac ggtaccgaac
     5881 aaatcctggt cgatcggaag cgcggttcgc aaaatcgtcg gcatgacaag caaatcggcg
     5941 cgctgcagca acgcttgcac ccgccggaac aggatggtgc ggtcgaacat cgcctgctgg
     6001 tagtcgacac cgctgatccg ctgcgccagc gccagctgct tgaggaaggt gtcgctcaac
     6061 tcgtcccggt gctcggccgc gagtttttca aatcgggttc gccagacggt gtgattgatc
     6121 gcgcgccaga tcggctcgac gtcaaagcca tcgccgggca tctcctcgag ttcagcgccg
     6181 agcgcttcga gcgttttcag gcttgcactg aacgccgccg caacatcggc ggaagccttg
     6241 cggcctggcg gcgcgaggca gaacagaatt cgtttcccgc tgaggtcgcc aaccggcaac
     6301 gcatcgccaa tgaaatcccg agcgggaatt ccgatcgacc aggggtcgga cgcatcctcg
     6361 ccagccatga cctgcatcat cagcgcagtg tcggcgaccg tgcgcgtggt cggggtgaca
     6421 taggtctgat tgccgaaggc gtccggcacc tgactatggg gaatcacgcc attgctctgc
     6481 ttcagcccga caacgccgtt gcaggcggcc gggatgcggg tcgaaccacc gccatcggtt
     6541 gccaccgcca gcggtgcaat gccgctcgcg accgccaccg ccgcgccgcc gctcgagccg
     6601 ccgctgctgc ggccgccgct ccaggcgttg cgggtgcggc caaacaaggg tgaatcggtc
     6661 aggcatttgg tgccgaattc cggcgtcgtg gtcttaccga taaggatcgc gcccgccgcg
     6721 cgcaaccgcg ccacggccac ggcgtcatgg tcggggacat tgtccctata cggaatggcc
     6781 ccgaaggtcg tgcggacgtc tttggtgttg acgatgtctt tgacggtaaa cgggatgccc
     6841 tccagaatcc gaagcggctc gccggccatg atcctgcgtt cggcgtcctt tgcttgagcc
     6901 aaggccttgt ccgcacagag tgtaataaag caatttagtt caggctgtaa tcgcttcgct
     6961 cgcgcgatta ccgcgcttgt gacttcggac ggcgaaatct ccttgcgact aatgcgccgt
     7021 cgcaggtcgg cggcggagag gaagcaaagt tcgtcactca tcttcaaact actcccaaag
     7081 actatccggc gaagccgga
//
LOCUS       DATNOI010000485        13719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_68275,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000485 DATNOI010000000
VERSION     DATNOI010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13719
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68275"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VN968_19375"
     CDS             complement(<1..351)
                     /locus_tag="VN968_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31431.1"
                     /translation="MTTTHQNRFWSWNSSDIEPDLPVGSITFGSATLASGTPSSFAAS
                     RRASALRPRDSSQRTDSGAIHAIKSEVTIGIAPVAATPRQPITGSRLAEINAANMVPS
                     ATGTTIMLETNVRYL"
     gene            391..936
                     /locus_tag="VN968_19380"
     CDS             391..936
                     /locus_tag="VN968_19380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL31432.1"
                     /translation="MGTHHLVSASEDTAHEAAHYFVYASIFSILGRVLFSFLPLYIGR
                     RGAALVGTFISVLVMLAVVAFYREFIAGWSVFALLVIFGAASYSGCFSNMSPYVVEVF
                     PVSLGGRAFGLAQAANGIGKILGPVCLALIAGSSDVVSPKATADAIAPAFLFLAACEC
                     AALIAIFAYRREPHGKPMDLG"
     gene            969..2591
                     /locus_tag="VN968_19385"
     CDS             969..2591
                     /locus_tag="VN968_19385"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXL31433.1"
                     /translation="MLDIPVLISGGGPVGLTASLLLSRHGVRSLLVERHPGTALTPKA
                     RGINARTMEIFRQCGIDAAVRAAGLAEGRLGLIVWTETLAGREIERRVPGRATPKNMA
                     VTPVKNCLCAQDDLEPVIRRFAEAAGPGTLRFNTELTSFSQRPGAVTGLLTDRMTGEE
                     TPFTARYLIAAEGAQSRVRRALAVKMIGEENVYDSVNILFHADLTQWVAHRSAALYFV
                     EQEDLRATFLTINGGDRWGFLIHGPKQYGWRPQDFTPEFCTELIRKAVGVEDLDVSVL
                     GVNPWQASAIVSDRYRVGDVFLAGDSAHEMPPTGGFGLNTGVQDVHNLAWKIAAVLFG
                     KADDKLLDSYHAERQPLGRMITQNSLANALSMGRTARQSNVLPRPEFLNEQGLIFGAC
                     YRSTAVVPDGTPPVAVDDPVTEYVPSARPGSRAPHVRLKRGDEQISTIDLFGPHFVLL
                     AGRDGDAWRRAAQGLGTSWPPLIAFTVGKDGDLSDPDGDWHDAYGVDPDGMVLVRPDG
                     HVAWRSRSGASNPLEVLRGAFDGLSGRMPAMA"
     gene            complement(2605..3198)
                     /locus_tag="VN968_19390"
     CDS             complement(2605..3198)
                     /locus_tag="VN968_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXL31434.1"
                     /translation="MSSISCARIAYVDIKANRFLLNAPSVSAAGVRNVIQLFAVALVA
                     ASLAACAQSSVVTGKSELNPASRHASLEQNRTTSFVTNRRVAVTRKHTPFASHKNAAE
                     TQVASHGLASFYTEGTQTANGERFDTRELTAAHRTLPFGTRLRVTNVATGRSVTVRVN
                     DRGPFVAGRVVDVSYSAAQTLGMVGGGLAKVKLDVVQ"
     gene            3430..4410
                     /locus_tag="VN968_19395"
     CDS             3430..4410
                     /locus_tag="VN968_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2778 domain-containing protein"
                     /protein_id="HXL31435.1"
                     /translation="MTYVAATVDKIPSGRGISFSGLVALIIVALAFGVAAWATDAVTW
                     VPDSVSSNDSNASSFDDRFVSGAASNLISMDLSRPFVQPLPSELEIKFQQAKARLAQK
                     LQSQDRRVAPVEEPAPSSVAVVPLPRSRPVEANLELRDPPLTAQPDNRTLLQKLSDLL
                     PARVTLASLAPDGGLLRDGPDLASLGYDNFTAVYDISARTVYMPNGLKLEAHSGFGSL
                     MDDPGHVGERNVGATPPNVYDLKPRERPFHGVQALRMIPVGAQDTLGRSGLLAHSYML
                     GANGGSNGCVSIRDYERFLKAFNNGEINRLVVVASLRDSQSAARRSVSQS"
     gene            complement(4480..5988)
                     /locus_tag="VN968_19400"
     CDS             complement(4480..5988)
                     /locus_tag="VN968_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXL31436.1"
                     /translation="MLKSSLPVRLALLVAGTTLPLIIFATVLVFNDYEQDRRDASQRV
                     LETVHSIRLVLDAEVQRMTGGLQVLTLTDALRNGDFENFRRIASGFLDQYGPDGVVLV
                     ANREGRQLFSSMTTNTASLPMRNNRDIVEKVFATKSPAYSNLFIGAVKKRPIMTVEVP
                     VLRDGDVIYDISFSPPISIFQTIIEKQRPSEDWTISLFDGNGVNFARIPNPHETIGKR
                     ASPSLYGEMLRRPEATLQTVSLEGVSLITSYARSSLTGWTVAAGIAESSLVGPLWRSL
                     AITSAIGGILLLTGLAFAVRMATTIARGKMLHDLLIEELNHRVKNTLAILQAVAVQTF
                     RSASTAEREKFEGRLGALAEAHNLLSMEKWHGSELQDVVSRVLQPYLLNNPERIRMSG
                     PRVPLSPRLAVVLSMLLHEMATNAAKYGALSNDAGTVALDWDVITENAGPKLRLVWTE
                     AGGPPVVAPVQRGFGSRLIERSARDQLGGEATVDFLPRGVVYTVTCALEGVS"
     gene            complement(6131..7405)
                     /locus_tag="VN968_19405"
     CDS             complement(6131..7405)
                     /locus_tag="VN968_19405"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXL31437.1"
                     /translation="MLAPTPASPESAGMSKAAFDRIEAHLKHRYVDAGRFPGTQLLVY
                     RRGKIVHSAVQGFADLERKAPMKDDTIFRIYSMTKPITSVAFMMLFEEGRVALDEPVH
                     KYIPEWKNLGVFQAGTWPAFLTKPPSRPMLIVDLLRHTSGLTYGFQQRSNVDAAYREL
                     KIGEVLKSGTLQSMIDDLSKTPLEFSPGEAWNYSVSTDVIGYLIGKISGQPFEQFLKE
                     RILSPLGMNDTDFLVPPDKVHRFAACYSADPKGGMTFHATDRKGSLTLQDDPANSSFL
                     SPPSLISGGGGLCSTAADYLTFCRALLGGGELGGVRLIGPKTLALMTSNHLPGGCDLP
                     EMSRSLFSEATYNGIGFGLGVSVTMDPAKTLIPGSRGEYAWGGAATTSFWIDPAEELI
                     AIFMTQVLPSSAYPLRRELRTMVYAAITDSNL"
     gene            7543..8454
                     /locus_tag="VN968_19410"
     CDS             7543..8454
                     /locus_tag="VN968_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HXL31438.1"
                     /translation="MPQFNDTELTAAVIRSFDETPDPRVKFLMEELVKSLHDYVRKTD
                     LTFDEWGYAIDFLTRVGQKCTPTRQEFILLSDVFGVSMLVDAVNHRERDGATETTVLG
                     PFYVGEHKPMPHGADISANLPGERMFVQSSVTDLKGKPLAGVPVDVWHADDDGYYDSQ
                     RPTYETDGPSSRARFVTDADGRFFFRTILPCSYPIPTDGPVGEMILQTRRHPMRPAHV
                     HFLVAAPGYEPLITHVFIDGDKYLDTDVVFGVKDELIARIEKRTDPVTPDGKPVTGPW
                     HLMTYAFRIKPGEGTAPKPMMAATEDA"
     gene            8781..10166
                     /locus_tag="VN968_19415"
     CDS             8781..10166
                     /locus_tag="VN968_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL31439.1"
                     /translation="MSQKDPTWISDWNPEDKTFWDSKGQVIARRNLIWSIVAEHIGFS
                     VWLIWSIVATKLPQAGFHYTTDQLFQLVAVPGLIGSLMRFPYTFAVTMFGGRNWTIFS
                     ASVLFIPTLALAYFVSQPDTPFWVMLLVASTAGLGGGNFASSMANISFFYPDRMKGWA
                     LGLNAAGGNIGVSSVQLLTPILMSVGFFSLYQATPTSDGIYLQNAGLMWILPLAVAVF
                     GGIFFMNNLTSAKSTFKDQLAIVGRKHTWIMSFVYIGTFGSFIGYSAAFPLLIKTQFP
                     AVTIAIAFMGPLVGSLSRPLGGLLADKVGGAIVTFWNFIAMGAATIGVLYFVGTKDFT
                     GFLVMFLILFVTTGIGNGSTYRMIPSIFREENLAKAKGKGEAGRALALRAAGIESGAA
                     LGFIGAIGACGGYLIPSGFGKSIAITGGPQLALVIYLAFYAVCLALTWWFYLRKSPAA
                     SGATSLAEARV"
     gene            10297..10722
                     /locus_tag="VN968_19420"
     CDS             10297..10722
                     /locus_tag="VN968_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HXL31440.1"
                     /translation="MTPDQVKLVQESFAKVAPISETAAMLFYNRLFEIAPQVKAMFPA
                     DMTEQRKKLMATLAFVVNGLGNLESILPAASALATRHVSYGARREHYPVVGAALLWTL
                     EQGLGDGWTPELVDAWTAAYGTLSGFMISEAYGRSQAAE"
     gene            10732..11949
                     /locus_tag="VN968_19425"
     CDS             10732..11949
                     /locus_tag="VN968_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL31441.1"
                     /translation="MSEPLIIVGNGMAAARLVDELAKAALGRYAIAVIGDEPRLAYNR
                     VLLSSVLAGETASHDIELRPASWWRDRGVTLKYGCVATEIDVGRRELKIANEESIGFS
                     RLVLATGSTPLRLNVPGAGLAGVHTFRDSRDVDLLLTLAAQRRRVVVIGGGLLGLEAA
                     YGLAKAGASVTLLHLMDRLMERQLDAPAAELLKSVVERKGIKILLNANTARLHGERRV
                     EGIELTDGRRIDADAVIFAAGIRPNIALAKSAGIPVGRGIVVDDCLQTGAPDIFALGE
                     CAEHRGICYGLVEPAYEQARVLARHLAGCAAAYSGSVVATNLKVSGVSVFSAGDFIGA
                     EGSEAIVLNDVRHGTYKKLVIADGRLVGAELVGDAGDALWYLELIRSREPIARIRNRM
                     MFGRSLALSSEAA"
     gene            11971..>13719
                     /locus_tag="VN968_19430"
     CDS             11971..>13719
                     /locus_tag="VN968_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXL31442.1"
                     /translation="MTAVDPTLRATRTTCPYCGVGCGVLATPDSGGAAAISGDPEHPA
                     NFGRLCSKGSALGETLGLGDRLLYPMIRCSKGTMERVAWTDALDHVAHRFAHIVKRDG
                     PDAVAFYLSGQMLTEDYYVANKLMKGFIGSANVDTNSRLCMASSVAGHRRAFGADTVP
                     GCYEDLDEADLLVLIGSNAAWCHPVLFQRMLANKQQRGARIVVIDPRRTDTAGDADLF
                     LGLKPGTDTALFSGLLVHLADTGTLDHDYIESHTSGFEAALARARSIAGSVGATALAT
                     GLAEPDVADFFQMFRNTPRVVTLYSQGVNQSAQGTDKVNAIINCHLATGRIGKPGASP
                     FSLTGQPNAMGGREVGGLANQLAAHMGFTPPDIDRVRRFWKAPRIATHEGLKAVQMFE
                     AIAQGQIKALWVIGTNPAVSLPDADAARASLKKLELFVISENVRSNDTVNAGAHVLLP
                     AQAWGEKSGTVTNSERRISRQRAFLTPPGESKPDWWIVSEVAKRLGFGAAFDFGSAAD
                     VFREHAALSAFENEGGRDFDIGALKSLSDEAFEAMAPVQWPTRSGDAEPQQRFFAAGG
                     FFANDHKARFVAPEVPA"
BASE COUNT         2492 a         4178 c         4377 g         2672 t
ORIGIN      
        1 cagataccgt acgttcgttt ccagcatgat ggtggtgccg gtcgcgctcg gcaccatgtt
       61 cgccgcgttg atttcggcga gcctgctgcc ggtgatcggc tggcgcggcg tcgcggccac
      121 cggtgcgatg ccgatcgtta cttcgctctt gatcgcgtgg attgcgccgg agtcggtgcg
      181 ctggctgctg tcgcgcggcc gcaatgccga tgcgcggcgc gaggccgcaa agctgctcgg
      241 ggttcccgag gccagcgtcg cgctgccgaa tgtgatcgag ccgaccggca agtcaggctc
      301 gatgtccgag ctattccacg accagaagcg attctggtgg gtcgtcgtca tctggatcgg
      361 gatttcgacc ggcacctatg gcgtgattct gtggggaccc accatcttgt ctcagcttct
      421 gaagataccg cgcacgaggc cgcgcattac ttcgtctatg cgagcatctt ctcgatactc
      481 ggccgcgtgc tgttttcctt cctcccgctc tatatcgggc gccgcggcgc ggcactggtc
      541 ggaactttca tctccgtcct ggtgatgctg gcggttgtcg ctttctatcg tgagttcatc
      601 gcgggctggt cggtgtttgc gctgctggtg atctttggcg cggcgtctta cagcggttgc
      661 ttctccaaca tgtcgccgta tgtggttgag gtgtttccgg tctcgcttgg cggccgggcg
      721 tttggattgg cgcaggccgc caacggaata ggaaaaatcc tggggccggt ttgtctggcg
      781 ctgatcgcgg gaagcagcga tgtggtttcg ccgaaagcga cggcggacgc gatcgcgccg
      841 gccttcctgt tcctggccgc ctgcgaatgt gcggcgctga tcgcgatctt cgcctatcgc
      901 cgggaacctc acggcaaacc gatggatctt ggataattca cgcgcacggg atccaaaaag
      961 gagaaaagat gctcgacatc cccgttctca tttccggcgg cggtccggtc ggcctcaccg
     1021 cctcattgct gctctcccgg cacggcgtgc gctcgctgct tgtcgaacgt cacccgggca
     1081 cggcgctcac gccgaaggcg cgcggcatca atgcccggac gatggaaatc ttccggcaat
     1141 gcggcatcga cgccgcggtc agagcggcgg ggctggcgga aggacggttg gggctgatcg
     1201 tctggaccga aaccctcgcc ggcagggaga tcgagcgccg ggtacccggt cgcgcgaccc
     1261 caaagaacat ggcagtgaca cccgtcaaaa actgtctttg tgcccaggat gacctcgagc
     1321 cggtcattcg tcgatttgcg gaagcagcgg gaccgggaac gctgcgcttc aataccgaac
     1381 tgacctcgtt cagccaacgg cccggtgccg tcacgggcct attgaccgac cgcatgacgg
     1441 gggaggagac gcctttcacc gcgcgctatt tgatcgccgc cgagggcgcg caaagccgcg
     1501 tgcgccgcgc gctggcggtg aaaatgattg gagaagagaa tgtctacgac agcgtcaaca
     1561 ttctgttcca cgccgacctc acccaatggg tcgcgcatcg gtctgccgcg ctctattttg
     1621 tcgagcagga ggacctgcgc gccacgttcc tcacgatcaa cggcggagat cgctggggct
     1681 tcctgattca cggccccaaa caatatggct ggcggccgca ggacttcacg ccggaatttt
     1741 gtacggaact gatccgcaaa gcggtcggcg tggaggacct cgacgtatcg gtgctgggcg
     1801 tcaatccctg gcaagcgtcc gccatcgttt ccgaccgcta tcgcgtcggc gacgttttcc
     1861 tggccggaga ttcggcccat gaaatgccgc cgaccggggg cttcggcctc aacaccgggg
     1921 tgcaggacgt gcacaatctg gcctggaaga tcgccgcggt gcttttcgga aaagccgacg
     1981 acaagctgct cgacagctac cacgccgagc gccagccgct tggccggatg atcacccaga
     2041 actcgctggc caacgccttg tcgatgggac gcaccgcgcg gcaaagcaac gtcttgccgc
     2101 gaccggaatt cctgaacgaa caggggctga tcttcggggc ttgctatcga tcgacggcgg
     2161 tcgtgccgga cggcacgcca ccggtcgcgg tcgacgatcc cgtcaccgaa tatgtgccct
     2221 ccgcccggcc cggcagccgc gcgccccatg tccgcctcaa gcgtggcgac gagcagatct
     2281 cgaccatcga cctgttcggc ccgcactttg tgctgctggc cggccgcgac ggcgacgcat
     2341 ggcgacgggc cgcgcaaggg ctcggcacgt cctggccgcc actcatcgcc tttacggttg
     2401 gcaaggacgg cgacctcagc gaccccgacg gcgactggca cgacgcttat ggcgtcgacc
     2461 cggatgggat ggtcctggtc cgaccggacg gccatgtcgc gtggcgcagc cggtcaggcg
     2521 cctcgaaccc gctggaagtt ttgcgcgggg cgttcgatgg cttgtccggc aggatgcccg
     2581 cgatggcgtg aggaagagtt ctggctattg gacgacgtca agtttgacct ttgcaagtcc
     2641 gcctccaacc atccccagcg tttgagccgc agaatacgag acgtcgacaa cgcgtccggc
     2701 aacgaacgga ccgcggtcat tgacccgaac ggtgacggac cgcccagtag cgacgtttgt
     2761 cacgcgcaac cgggtgccga acggcagcgt gcgatgagcg gcggttaatt cgcgggtatc
     2821 gaacctttca ccgttcgcgg tctgtgttcc ctctgtgtag aagctggcaa gtccatgcga
     2881 cgcaacctgt gtttcggctg catttttgtg cgaagcgaac ggggtatgct ttctcgtgac
     2941 cgccacacgc ctattcgtca cgaatgatgt tgttcgattc tgctccagcg atgcgtgtcg
     3001 actggcagga tttaattcgg attttccggt gaccaccgag gactgggcgc aggccgcaag
     3061 cgaagccgcg accagagcca cggcaaacaa ttgaatgacg ttccgaacac cagcggccga
     3121 aaccgaaggc gcgttaagca gaaaacggtt tgccttaatg tcgacgtacg caattctggc
     3181 acagctgatg gaagacatgt tgcaccctcc aacccaccga aatccgacag gtaacaaggg
     3241 cggaacgcaa gggcggcact atggccagat gaaaaccggc aggggatcaa tcacacaatt
     3301 catctgagtg tggcgattgt gtcacaactt atcgcaaata cctcaatctg ccagcgattg
     3361 cctcgattta gacgtattcc ggcgagcacg atgttggctc ggttgcaagg cgtccgacgg
     3421 gtgtactgga tgacgtacgt cgcagcgaca gttgataaga ttccgtcggg tcgcggcatt
     3481 tcgttctccg gcctggttgc attgatcatc gttgcattgg cattcggagt agcagcctgg
     3541 gctaccgatg cggttacctg ggtcccagat tcggtttcct cgaatgacag caacgcgtca
     3601 tcgttcgatg accgtttcgt ctcaggtgca gcgtccaatt tgatttcgat ggacctatcg
     3661 cgacccttcg ttcaaccttt gccgtcagaa ctcgagatca agtttcagca agcgaaagcg
     3721 cggcttgcgc agaagctgca gtctcaagac cggcgggtag cccctgtgga ggaacccgcg
     3781 ccatcaagcg tcgctgtcgt tccccttccc aggtctcgtc cggttgaggc gaacctcgaa
     3841 ttacgagacc ctccccttac tgctcaacct gacaatcgca cgctgctgca gaagctttcc
     3901 gatctgctcc cggcccgtgt cacgcttgcg tctctggctc cggatggcgg gctcttgcgc
     3961 gacgggccag atcttgcgtc gcttggctat gacaatttca ccgccgtcta tgacatctcg
     4021 gcgcgcactg tttacatgcc aaacgggttg aagctcgagg cgcattcagg atttggtagt
     4081 ttgatggatg atccaggaca tgtgggcgag cgaaatgtcg gagcgacgcc accaaatgtc
     4141 tatgatctga aaccgcggga gcgaccgttt cacggcgtcc aagcgctccg catgattcct
     4201 gttggcgccc aagatacgct cgggcggtcc ggattgctcg cgcacagcta tatgctcgga
     4261 gcgaacggcg gttctaacgg ctgcgtgtcg atcagggact atgagagatt tttgaaagcc
     4321 ttcaacaacg gtgagatcaa tcgcctcgtc gtggtggcga gcctccgtga cagccagtca
     4381 gcagcgcgac gatccgtttc acaatcgtga tgcaggaact gttgccttcc aaagcatgat
     4441 gacagatgga tttgttcgct gcgagcgcat agttctgcgc tagctcacgc cctccagcgc
     4501 gcaggtcacc gtgtagacca cgccgcgggg cagaaaatcg accgtggctt cgccgccgag
     4561 ttgatcgcgc gcgctgcgtt cgatcagccg cgacccgaaa ccgcgctgca ccggcgcgac
     4621 cacgggaggg ccgccggcct cggtccagac cagtcgcagt tttggcccgg cgttttccgt
     4681 aatgacatcc caatccagcg caacggtgcc ggcgtcgttc gacaacgccc catactttgc
     4741 ggcgttggtc gccatctcat gcaagagcat cgacagcacc accgcaagcc gcggcgatag
     4801 cggtacgcgt ggtccggaca tccggatccg ctccggattg ttgagcagat agggctgcaa
     4861 cacacggctg accacgtcct gcagctcgga gccatgccac ttctccatgc tcagcagatt
     4921 gtgcgcctcc gccagcgcac cgagccggcc ctcgaatttc tcgcgttcgg ccgtgctggc
     4981 gctgcgaaag gtctgcaccg cgaccgcctg caggatcgcc agcgtgttct tgacgcggtg
     5041 attgagttct tcgatcaaga ggtcatgcag catcttgcca cgggcgatcg tcgtcgccat
     5101 ccgcaccgcg aaggcaaggc cggtcaatag caggatgcct ccgatcgcgc tggtgatcgc
     5161 gaggctgcgc cacaacgggc cgaccagcga gctttccgcg atcccggcgg ccacggtcca
     5221 gccggtcagc gaggatcgcg cgtacgaagt aatcagcgat acgccttcga gcgataccgt
     5281 ctgcagggtt gcttcgggcc tgcgaagcat ctcgccatac agcgagggcg atgcgcgctt
     5341 gccgatggtc tcgtgcgggt tgggaatacg cgcgaaattg acgccgttgc catcgaagag
     5401 agagatcgtc cagtcttcgc tgggacgctg cttttcaatg atcgtctgga aaatcgagat
     5461 cggcgggctg aaggaaatgt cgtaaatcac gtcgccgtcg cgaagcaccg ggacctcgac
     5521 cgtcatgatc ggtcgcttct tgaccgcgcc gatgaacagg tttgaatagg ctggactctt
     5581 ggtcgcgaac accttctcga cgatgtcgcg gttgttgcgc atcggcaagc ttgcggtgtt
     5641 cgttgtcatc gaggagaaca attggcggcc ttcccggttc gccaccagca cgacgccatc
     5701 cgggccgtat tggtcgagaa agccggaggc aatgcgacgg aaattctcga aatcgccgtt
     5761 gcggagcgcg tcggtgagcg tcagcacctg cagcccgccg gtcatgcgct gaacctcggc
     5821 gtccagcacg aggcggatgc tgtgcacggt ttcgagcacg cgctgcgacg catcccttcg
     5881 gtcttgctcg tagtcgttga aaacgaggac tgtcgcgaaa atgatcagcg gcagggtggt
     5941 tcccgcgacc agaagagcaa gacgcactgg cagtgaggat tttagcacgc gggtcctgca
     6001 tcaaggcact tggcgcgggt ggcgccaaat caccaccagc ataggagaac cgcctgaaat
     6061 ccgccaagga ttttgggctc gcggcccggt ttcaaccggc cttgggtgcg atgcggaagt
     6121 tgcgccgctg ctacaaattg ctgtcggtga tggccgcgta aaccatggtg cgcaactcgc
     6181 gccgcagcgg ataggcgctc gacggcagca cctgggtcat gaagatggcg atcagttctt
     6241 ccgccggatc gatccagaac gatgtcgttg cagcgccgcc ccaggcatat tcgccgcggc
     6301 tgccggggat caacgtcttg gcgggatcca tcgtgaccga aacaccgagg ccgaagccga
     6361 tgccgttata ggtcgcctcg gagaacagcg accgcgacat ttccggcaaa tcgcatccgc
     6421 cgggcagatg gttcgacgtc atcagcgcca gcgtcttcgg accgatcagc ctgacgccgc
     6481 cgagttcgcc gccgcccagc agcgcgcggc agaaggtcag atagtcggcg gcggtcgaac
     6541 acaacccgcc gcctccggag atcaacgacg gcggcgacag gaaagaactg ttggccgggt
     6601 catcctgcag cgtcaggctg cctttgcggt cggtggcatg aaacgtcatg ccgcctttcg
     6661 ggtcggccga gtaacaggcc gcgaaacgat ggaccttgtc ggggggcacg agaaagtcgg
     6721 tatcgttcat gccgagcgga ctgaggatcc gctccttcag aaactgctcg aacggctggc
     6781 cgctgatctt gccgatgaga tagccgatca catcggtgga gaccgaatag ttccaggcct
     6841 cgccgggcga gaattccagc ggcgttttgg agaggtcgtc gatcatcgac tgcagcgtgc
     6901 ccgatttcag gacctcgccg attttcaatt cgcggtaggc ggcatcgacg ttggagcgct
     6961 gctgaaagcc ataggtcagg cccgaggtgt gccgcagcag gtccacgatc agcatcggcc
     7021 gcgagggcgg ctttgtcaga aacgccggcc aggtcccggc ctggaaaacg ccgaggttct
     7081 tccattccgg gatatattta tggacgggct cgtcgagcgc gacgcggcct tcctcgaaca
     7141 gcatcatgaa ggcgaccgat gtgatcggct tggtcatcga atagatgcga aagatggtgt
     7201 cgtccttcat cggcgccttg cgctcgaggt cggcaaagcc ctgcaccgcg ctgtggacga
     7261 tcttgccgcg gcggtacacc aacagctgcg tgcccgggaa gcggccggcg tcgacgtatc
     7321 ggtgcttcag atgcgcctcg atgcggtcga aggcggcctt ggacattccg gcggattcgg
     7381 gcgaggcggg ggtaggggct aacatcggac gggtctccag gggcgagggt tgccttgata
     7441 gccgaaaagt gtgtcgtgtt ccaatcgcag ccccgcttac cccgggctgt cgactggtgt
     7501 aggcttacag ccttggatcg cgtaccccgc aggacaatga ccatgcccca attcaacgac
     7561 accgagctta ccgccgccgt gatccgcagc tttgacgaga cgccggaccc gcgggtcaaa
     7621 ttcctgatgg aagaactggt gaagtcgctg cacgactacg tgcgcaagac cgatctcacc
     7681 ttcgacgaat ggggctatgc gatcgatttt ctgacccgcg tcggccagaa gtgcaccccg
     7741 acccggcagg aattcatcct gctgtcggac gtattcgggg tttcgatgct ggtcgatgcg
     7801 gtgaaccatc gcgagcgcga cggcgccacc gagaccaccg tgctcggtcc gttctatgtc
     7861 ggcgagcaca agccgatgcc gcacggcgct gatatctccg ccaatctgcc gggcgagcgg
     7921 atgtttgtgc agagcagcgt gaccgacctc aagggcaaac cgctcgcggg cgtcccggtg
     7981 gacgtctggc acgccgacga tgacggctat tacgattcgc aaaggccgac ctacgaaacc
     8041 gacgggccgt catcgcgggc gcgctttgtt accgacgcgg acggaaggtt cttctttcgc
     8101 accatcctgc catgcagcta tccgatcccg accgacggtc cggtcggcga gatgatcctg
     8161 cagacccggc gtcatccgat gcggccggca catgtgcatt ttctggtcgc ggcacccggc
     8221 tacgagccgc tgatcactca cgtctttatc gatggtgaca aatatctcga taccgacgtg
     8281 gtgttcgggg tcaaggacga gctgatcgcc agaatcgaga agcgcactga tccggtgacg
     8341 cccgacggca agccggtgac cgggccatgg catttgatga cctacgcttt ccgcatcaag
     8401 ccgggtgaag gaaccgcccc gaagccgatg atggctgcga ctgaggacgc ctgaattcat
     8461 tttttgggca cttgctcagt tcttgtgcga cgcacaagaa ttgagcgaat cgtaattcaa
     8521 gaaataagcg aatcgcaaat tttggatgcg ggctgacccc tgctaaaatt taggcgaacg
     8581 acgtaagtcg ttgaaaaaca tccaattttt tagatgcggt agattggcat gaagcttgaa
     8641 tggctgatgc tgacgccatc gatgccgtcc ttcgaaagcc aattatccga aattttgacg
     8701 ccgcaaaccg gggcttcccg gggcgcgctt gcatgccttc aggcgaccgc gaccagccgg
     8761 gcagaaagga aatctctcca atgagccaga aagacccgac ttggatttca gactggaatc
     8821 cagaggataa gacgttttgg gattccaagg gccaagtgat cgcccgccgc aatctcatct
     8881 ggtcgattgt cgccgagcat atcggatttt cggtctggct gatctggagc atcgtcgcaa
     8941 ccaagttgcc gcaggctggc ttccactaca ccaccgacca attgtttcag ctggtggcgg
     9001 tgcccggact gatcggctcg ctcatgcgat ttccctatac cttcgcggtt acgatgttcg
     9061 gcggccgcaa ctggacgatc ttcagcgcgt cggtcctgtt tatcccgacg ctcgcgctcg
     9121 cctatttcgt gagccagccc gacaccccgt tctgggtgat gctgctggtg gcctccacgg
     9181 caggtctcgg aggcggcaac ttcgcctcga gcatggccaa tatctccttc ttctatcccg
     9241 accgcatgaa gggctgggcg cttggcttga acgcggctgg cggcaatatc ggcgtcagca
     9301 gcgtccaatt gctgacgcca atcctgatga gtgttggttt tttcagcctc taccaggcga
     9361 ccccgacctc ggacggaatc tatcttcaga acgccggact gatgtggata ttgccgctgg
     9421 cggtggcggt gttcggcggc attttcttca tgaacaacct gacctcggcc aagtccacgt
     9481 tcaaggacca gctcgccatc gtcggccgca agcatacctg gatcatgtcc ttcgtctaca
     9541 tcgggacttt cgggtccttc atcggttact ccgccgcgtt tccgctgctg atcaagaccc
     9601 aattcccggc ggtcacgatt gcgattgcgt tcatggggcc gctggtgggc tcactgtcgc
     9661 gtccacttgg cggattgctg gctgacaagg tcggcggcgc gatcgtgacg ttctggaatt
     9721 tcatcgcgat gggcgccgcc acgatcggcg tcctgtattt cgtggggacc aaggacttca
     9781 cggggttcct ggtgatgttc ctcattctgt tcgtcaccac cggaatcggc aacggatcga
     9841 cctaccggat gattccgtcg atcttccgcg aggagaacct tgccaaggca aagggcaagg
     9901 gcgaagccgg ccgggcgctc gccttgaggg ctgcgggcat cgaaagcggc gccgcgctcg
     9961 gattcatcgg cgcgatcggc gcctgcggcg gttacctgat cccgagcggc ttcggcaagt
    10021 cgatcgccat caccggcggt ccgcagctgg cacttgtgat ctatctggcg ttctacgcgg
    10081 tctgcctggc gctgacctgg tggttctacc tgcgcaagag ccccgcggcg tcgggtgcga
    10141 cgagcctcgc cgaagcgcgc gtgtgaaccg catcgatagc ggccgacgcc aacgcggcga
    10201 ggtgcgtcgg aattgacagt gtttcttaac ctctccccgc aagcggaccg caagcgggga
    10261 gaggatgaga aggtggccga gcctggaggg aaaaaaatga caccggacca agtcaaactg
    10321 gttcaggaga gtttcgccaa ggtcgcgccg atctcggaga ccgcggcaat gttgttctat
    10381 aaccgcctgt tcgagatcgc gccgcaggtg aaagcgatgt ttccggccga tatgaccgag
    10441 cagcgcaaga agctgatggc gacgctggcc tttgtcgtga acggccttgg caatctggag
    10501 tccattctgc cggcggcgag cgcactggca acgcgccacg tcagctacgg cgccaggagg
    10561 gagcactatc ccgtcgtagg cgccgccttg ctgtggacgc tggagcaggg gctcggagac
    10621 ggctggacgc cggaactggt ggacgcctgg accgcggcgt acgggacgct gtccggcttt
    10681 atgatctccg aagcctatgg tcgctcccaa gccgccgagt aaggacacgc cgtgagcgaa
    10741 ccactgatta tcgtcggcaa cggaatggct gccgcgcgcc tggtcgacga actggcgaaa
    10801 gcagcgcttg ggcgttacgc catcgccgtg atcggcgacg agccgcggct ggcctataat
    10861 cgcgtgctgc tgtcgtcggt gctggcgggc gagaccgcgt cgcatgacat cgagctcagg
    10921 ccggcgtcct ggtggcgcga ccgcggcgtt accctgaaat atggctgcgt tgccaccgag
    10981 atcgatgtcg gccgccgcga actcaagatc gcaaatgaag aaagcatcgg attttcgcgc
    11041 ctggtgctcg ccaccggctc gacgccgctg cggctgaacg tgcccggcgc cggtctcgcc
    11101 ggggtgcata cctttcgcga tagccgggac gtcgatctgt tgctgacgct cgccgcgcag
    11161 aggcgacgcg tcgtggtgat cggcggcgga ctgcttggac tggaggcggc ctacggcctc
    11221 gccaaggcgg gcgcatccgt gacgctgctc catctgatgg accggctgat ggagcgccag
    11281 ctcgatgcgc ccgcggcgga actgctgaaa tccgtcgtcg aacgcaaggg catcaagatc
    11341 ctgctgaatg ccaacaccgc gcgccttcat ggcgagaggc gcgtcgaggg catcgaattg
    11401 accgacgggc gccggatcga tgccgacgcg gtgattttcg cggcgggcat ccggccgaat
    11461 atcgcactgg cgaaatcggc cggcattccc gtgggtcgcg gcatcgtcgt cgacgattgt
    11521 ctgcagaccg gcgcgccgga tattttcgcg ctcggcgaat gcgccgagca tcgcggcatc
    11581 tgttatgggc tggttgagcc agcttacgag caggcgcggg ttctggcgcg gcatctcgcc
    11641 gggtgtgccg cggcctacag cggcagcgtg gttgcgacca atctgaaggt ttcgggcgtc
    11701 agcgtgtttt ccgcgggcga tttcatcggc gcggagggaa gcgaagccat tgtcctcaac
    11761 gatgtcaggc acggcaccta caagaaactg gtgatagcgg atggacgtct tgtcggcgcg
    11821 gagctggttg gcgatgccgg ggatgcgctg tggtacctcg aattgatccg cagccgcgag
    11881 ccgatcgccc ggattcgcaa ccgcatgatg ttcggacgct cgctcgcgct ctcgtcggag
    11941 gcggcatgac ccgatgaccg gattgttcac atgacggctg tcgatcccac gcttcgcgcc
    12001 acccgcacga cctgtcccta ttgcggcgtc ggctgcggcg tgctggcgac gcccgacagc
    12061 ggcggcgctg cggcgatctc gggtgatccc gagcatcccg ccaatttcgg acggctgtgc
    12121 tcgaagggtt cggcgctcgg cgaaaccctc ggccttgggg accggttgct gtatccgatg
    12181 atccgctgca gcaagggcac catggagcgg gtggcctgga ccgatgccct cgaccatgtc
    12241 gcgcatcgct ttgcgcatat cgtcaagcgc gatggcccgg acgcggtggc gttctatctc
    12301 tccgggcaaa tgctgaccga ggattattat gtcgccaaca agctgatgaa ggggttcatc
    12361 ggcagcgcca atgtcgatac caattcgcgg ctgtgcatgg cgtcgtcggt cgccggccac
    12421 cgccgtgcat tcggcgcgga taccgtgccc ggctgctatg aggatctcga cgaggccgac
    12481 ctgttggtgc tgatcggctc caacgcggcg tggtgccatc cggtcctgtt tcagcgcatg
    12541 ctcgccaaca agcagcagcg cggcgcgcgc atcgtcgtga tcgatccgcg ccggaccgat
    12601 accgctggcg atgccgacct gttcctgggc ctgaagcccg gcaccgacac tgcgttgttc
    12661 tcgggtctgc tggttcatct cgccgacact ggcacgctcg atcatgacta catcgaaagc
    12721 cacacctccg gcttcgaagc cgcgctggcg cgggcgcgga gcatcgccgg cagcgtcggc
    12781 gcgacggcgc tggcaaccgg tctcgccgag ccggatgttg ctgacttctt ccagatgttc
    12841 agaaacaccc cgcgggtggt cacgctgtat tcgcagggcg tcaaccagtc ggcccagggc
    12901 accgacaagg tcaacgccat tatcaattgc catctcgcca ccgggcggat cggcaaaccg
    12961 ggcgcctcgc cattctcgct caccgggcaa cccaatgcga tgggcgggcg cgaggtcggc
    13021 gggcttgcca atcagctcgc cgcgcacatg gggttcacgc cgccggatat cgaccgcgtc
    13081 cgccggttct ggaaggcgcc gcggatcgct acccatgagg ggctgaaggc ggtccagatg
    13141 ttcgaggcga tcgcgcaggg gcagatcaag gcgttgtggg tgatcggcac caacccggcg
    13201 gtgtcgttgc cggacgccga cgccgcccgc gcgagcttga aaaagcttga gctgttcgtg
    13261 atctcggaaa atgtgcgctc caacgacact gtcaatgccg gcgcgcatgt gctgctgccg
    13321 gcgcaggcct ggggcgagaa atcgggaacg gtgaccaatt cggaacggcg catctcgcgg
    13381 cagcgcgcgt tcctgacccc gccgggcgag tcaaaaccgg attggtggat cgtcagcgaa
    13441 gtggcaaaac ggctgggctt cggcgcggca tttgatttcg gctcggcggc ggatgtgttt
    13501 cgcgaacacg ccgccctgtc ggcattcgag aatgagggcg gccgcgattt cgatatcggc
    13561 gcgttgaagt cgctatcgga cgaggcgttc gaggcgatgg cgccggtgca gtggccgacg
    13621 cgctccggcg atgccgagcc gcagcagcgg tttttcgcgg caggcggatt ttttgccaac
    13681 gaccacaagg cacgctttgt cgcgccggaa gttcccgcg
//
LOCUS       DATNOI010000486         5634 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1804707,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000486 DATNOI010000000
VERSION     DATNOI010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804707"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1011
                     /locus_tag="VN968_19435"
     CDS             25..1011
                     /locus_tag="VN968_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipo-like protein"
                     /protein_id="HXL31443.1"
                     /translation="MGVVLDTIGKLIADYLQKEIPGYEPFTPSDPEHLRGVIEAGDVM
                     LVEGNNRVSGIIKYLTQSTWSHAALFVGPIDGAAEPDGEPHVLIEANIGEGVTSAPLS
                     KYYPYHTRLCRPVGLSFEDRHTVCRYAINRIGFGYDTKNIVDLMRYLIPLPVPQRWRR
                     RIIAFGSGDPTKIICSALIAQAFDAVRYPILPKITRAASKKARREILHIRDSSLYMPR
                     DFDISPYFEIVKPTIVQGFDYTALHWADKQKPLQEVAGEFGVFPETISPPIVPEAIDE
                     EAPISVEEVILGAEPATLVERVVVSEHFMVVEYVAVHQPERRAHPREPVIAA"
     gene            complement(1173..1787)
                     /locus_tag="VN968_19440"
     CDS             complement(1173..1787)
                     /locus_tag="VN968_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXL31444.1"
                     /translation="MGFYSDIIVPRLCDFAMRNKQLVPFRERVIGAAEGRVLEIGVGS
                     GMNLPFYRPTVREVLALEPASRLVDMARSAAGGASMPVSFLEASAEAIPLDEHSVDTI
                     VTTWTLCSIPQAGTALAEMRRVLRPRGKLLFVEHGLAPDEGVRRWQDRLTPAWRRISG
                     GCHLNRPIRSMIEGSGFRIDRIETGYIPGPKPMTFMYEGSARRH"
     gene            complement(1870..2646)
                     /locus_tag="VN968_19445"
     CDS             complement(1870..2646)
                     /locus_tag="VN968_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HXL31445.1"
                     /translation="MNEMSALEYLLRRDRLIVAVGLAAVVALAWIYLATGAGMDTSMA
                     DMPMDMPMPWSPLSAALVFVMWWVMMIAMMVPSAAPTVLLFTTIRRKQEASVRPSVEA
                     WIFLGGYLLIWAGFSLIAIVAQWGLERAGLLSMAMASTTAMLGGVILLAAGLYQFTPV
                     KNACLRYCQSPVLFLSRYWRPGAGGALRMGLRHGSYCLGCCWFLMALLFVSGVMNLVW
                     IAGIALYVACEKLLPLGRGLSRAAGVALIVSGAIVLARAS"
     gene            complement(2702..3334)
                     /locus_tag="VN968_19450"
     CDS             complement(2702..3334)
                     /locus_tag="VN968_19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HXL31446.1"
                     /translation="MAASDWRLEGEWIKNCNCAFGCPCDFNAPPTHGSCKGILGMRIT
                     KGHFEGTKLDGLVFMVTVDFPGPLHEGNGKIQPIIDERATPEQRQALFNIMSGKNSAE
                     GTLFQILSLIVAKIHDPVFVPFEFSFDKNGRVARVVAKGVLETEVEPIKNPVTGAPHR
                     IQVVMPDGFEHRSAEIASANIHSTGAIKFDTKGTHSSLAKVVQTPDGVAA"
     gene            complement(3610..>5634)
                     /locus_tag="VN968_19455"
     CDS             complement(3610..>5634)
                     /locus_tag="VN968_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HXL31447.1"
                     /translation="GGIAIAVGAMIDAAIVMIENAHKHLERADPGRSRVDVLIEAAGE
                     VGPALFFSLLIITVSFMPIFTLESQEGRLFSPLAFTKTFSMAAAALLSVTLVPALMVI
                     CIRGRIVPEHRNPINRFLIWIYRPVIACVMRAKVLVILISLAVLAVTIWPARQLGSEF
                     MPSLNEGTLLYMPTTLPGISVTKAAELLQTQDKIIRSFQEVASVYGKAGRASTATDPA
                     PSEMFETIVNLKPKQEWRPGVTIESLTAEMDRALQFPGVSNAWTMPIRARIDMLSTGI
                     RTPVGGKVIGTDLVEIDRLAKQIEQVLKTVPGTSSAYAERALGGYYLDVTPDRAALAR
                     YGIMVQDVQDVIATALGGQTVTTTVEGRQRFSVNMRYPRDLRSNAQAIASDVLVPMPN
                     GGAVPLGEVAKIAPSRGPTSIRTENGQLAVYIYVDIRDRDLGGYVADAQNAVNASIAF
                     PAGSYVVWSGQFEYLERAAARLRIVVPVTLSIIFLLLYLNFRSFADTMIVMLSLPFAL
                     VGGIWLMWWLGFNLSVAVVVGFIALAGVAAETGVIMLIYLNHALAEVQAHRDADGNRL
                     SRQDLYDAIMKGAVERVRPKMMTVVAIMAGLLPIMWNTGTGSEIMQRIAVPMIGGMIS
                     STLLTLIVIPAIYGIVKGFRLPREDRLDSQPPSLPSKSIKRPQPVSEPAE"
BASE COUNT         1133 a         1795 c         1699 g         1007 t
ORIGIN      
        1 gaacgcccgc tgggagaaaa tgtgatgggc gttgtgctcg ataccatcgg caagctgatc
       61 gcggactacc ttcagaagga aataccgggc tatgagccgt tcacgccgag cgaccccgaa
      121 catttgcgcg gcgtcatcga ggccggcgat gtcatgctgg tcgaaggcaa caaccgggtt
      181 tccggcatca tcaaatatct cacccagtcc acctggtcgc atgccgcgct ttttgtcggc
      241 ccgatcgacg gcgcggccga gcccgacggc gagccgcatg tcctgatcga ggccaatatc
      301 ggcgagggcg tgacgtcggc cccgctgtcg aaatattatc cctatcacac gcggctgtgc
      361 cgcccggtgg gcctgtcgtt tgaagatcgc cacacggtgt gccgctacgc catcaaccgc
      421 atcggcttcg gctacgacac caagaacatc gtcgacctga tgcgctatct gattccgctg
      481 ccggtgccgc agcgctggcg gcggcggatc atcgcgttcg gctccggcga tcccaccaag
      541 atcatctgct cggcactgat cgcacaggcg ttcgacgccg tgcgttaccc tattctgccc
      601 aagatcaccc gtgccgccag caagaaagcc cgacgcgaaa ttctgcacat ccgcgattcc
      661 tcgctgtaca tgccgcgcga tttcgacatc tcgccctatt tcgaaatcgt caaacccacc
      721 atcgtgcaag gcttcgacta caccgctttg cactgggccg acaagcaaaa gccgctccag
      781 gaggtagcag gcgaattcgg cgtgtttcca gaaacgatct cgccgccaat tgttcctgaa
      841 gcgattgacg aagaagcgcc gatttcggtt gaagaagtaa tcttgggtgc cgagccggcg
      901 acgctggtcg agcgtgtcgt cgtgtcggag catttcatgg tcgtcgaata cgttgctgtg
      961 catcagccgg agcgtcgcgc ccacccgcgc gagccggtga tagcggctta ggttttcgcg
     1021 aattccaccc gacaataatc gacgtgagaa ccgccgcagg cgcgatgccg gggattgcaa
     1081 atggcagggt ctggcgttca gcctggatcc cgcgacctat ggcggatagc caggcgccat
     1141 gtaccggtac gaactgtacc gcatccgtct cgtcagtgcc gccgcgcgct gccttcatac
     1201 atgaacgtca tcggcttcgg tccggggata tagccggttt cgatgcggtc gatgcggaag
     1261 ccactccctt cgatcatgga acggatcgga cggttgaggt gacagccccc gctgatacgc
     1321 cgccaggccg gcgtcagccg atcctgccat cggcgcacgc cctcgtcggg ggccagaccg
     1381 tgctcgacga acagcagctt gccgcgcggc cgcaatacgc gccgcatctc ggcgagcgcg
     1441 gtgccggcct gcgggatcga gcacagggtc caggtcgtga cgatggtatc gacgctatgc
     1501 tcgtcgagcg gaatggcctc ggcggaggcc tcgaggaagc tgactggcat gctggcccca
     1561 ccggcagcgc tacgagccat atccaccagc cgcgatgccg gttcgagcgc gagcacctcc
     1621 cgcacagtcg ggcgataaaa cggcaggttc attcccgagc cgacaccgat ttcgagcact
     1681 ctgccctccg ccgcgccgat cacgcgttcg cgaaacggca cgagctgctt attgcgcatc
     1741 gcgaaatcgc aaagcctcgg cacgatgatg tcgctataaa agcccatcga tcactcctga
     1801 atgttccctc aacgcgacga cgtcgcgaac acgttgacag tggcttcgca ttgccgggcg
     1861 cgctttttct caacttgcgc gcgcaagaac aatcgcgcct gatacgatca gggccacacc
     1921 ggcggcgcgg ctgagcccgc ggccgagcgg cagcaacttc tcgcacgcga catagagggc
     1981 gatgccggca atccagacaa ggttcatgac cccgctgacg aacagcagcg ccatcaggaa
     2041 ccagcaacag ccgaggcagt agctgccgtg ccggagcccc atgcgcaatg ctccccccgc
     2101 gccagggcgc cagtacctgc tcaggaacag caccgggctc tggcagtagc gcaggcaggc
     2161 gtttttgaca ggcgtgaact ggtagagccc tgcggcaagc aggatgacgc cgccaagcat
     2221 ggcggtggtg ctcgccatcg ccatagacag cagcccggct ctttcgagcc cccactgcgc
     2281 cacgatggcg atgaggctga aacctgccca tatcaggaga tagccaccga gaaagatcca
     2341 ggcttccacg gaaggacgaa ccgaggcctc ttgcttgcgc cggattgtcg taaacaacag
     2401 gaccgtgggg gcagcactcg gcaccatcat ggcgatcatc atcacccacc acatgacgaa
     2461 gacgagcgcg gctgacaacg gcgaccacgg catcggcata tccatcggca tgtcggccat
     2521 gctcgtgtcc atgcccgccc cggtcgccag atatatccac gccagcgcca ccactgcagc
     2581 cagccccacg gcaacgatca gccggtcgcg ccgcagcagg tattcgagcg cggacatctc
     2641 gttcatgggc ccacctcggc cgccgccgcg acaagtcgaa agagcgaacc ttagcgcagc
     2701 atcaagccgc aacgccatca ggcgtctgta cgactttggc gagcgagctg tgcgtgcctt
     2761 ttgtgtcgaa cttgattgcc ccggtcgagt gaatgttggc ggaggcaatc tcggcggatc
     2821 tgtgctcaaa gccgtcgggc atcacaactt gaatgcgatg gggagcgccc gtcaccgggt
     2881 tcttgatcgg ctcgacctcg gtctcaagca cgcccttggc gacaacacgc gcgacgcgac
     2941 cgttcttgtc gaatgaaaac tcgaagggca cgaataccgg atcatggatc ttggctacga
     3001 tcaggctcaa gatctggaac agcgtgccct cggccgaatt cttgcccgac atgatgttga
     3061 aaagcgcctg ccgctgttcc ggtgtggcgc gctcatcgat gatcggctga atcttcccat
     3121 tgccctcgtg cagcggtccc gggaaatcga cagttaccat gaacacgaga ccatcgagct
     3181 tggtaccctc gaagtgtccc ttggtaatcc tcatgccgag gatgcccttg cacgagccgt
     3241 gagtcggcgg ggcattgaaa tcgcaggggc aaccaaacgc gcaattgcag ttcttgatcc
     3301 attcgccttc gagacgccaa tcagatgcag ccatggtaca cctcccatcg cgttgtcagg
     3361 ttgtcagctg gaccttgcag tcgccgcttg attatcctct tcagcgctga gaagccgggg
     3421 tcaatgcgag cagaatctgc attgctccgc ggaggcttga gacggctagc ggactgcgat
     3481 ttgctatcct gatcatagaa gcataccagc caacgccctc agcgtacagg cgggccatag
     3541 atggtaacag gatatccttg cgcaccccgc gtccttgcgt ccagaacgtt ccgggcatca
     3601 tcggtcacct cattcggctg gctccgaaac aggttgaggt cgcttgatcg acttggacgg
     3661 caatgaagga ggctggctgt cgaggcggtc ttcgcgcggc aaccggaacc ctttgactat
     3721 cccatagatc gccgggatca cgatcagcgt cagcagcgtc gatgagatca tgccgccgat
     3781 catcggcacc gcgatccgct gcatgatctc ggagccggtg ccggtgttcc acatgatcgg
     3841 cagcaggcct gccatgatgg caaccaccgt catcatcttg ggacgcaccc gctcgactgc
     3901 gcccttcatg atcgcatcat acagatcctg gcggctcaac cggttgccgt cggcatcgcg
     3961 gtgagcctgg acctccgcca gcgcgtggtt gagatagatc agcatgatca cgccggtctc
     4021 ggcggcgacg ccggcaagcg cgatgaagcc gacgacgacg gcgaccgaaa ggttgaagcc
     4081 gagccaccac atcagccata tcccgccgac cagggcgaag ggcagtgaga gcatcacgat
     4141 catcgtatcg gcgaacgagc ggaagttgag atacaaaagc aggaagatga tcgagagcgt
     4201 caccggcacc acgatcctca gacgcgccgc cgcgcgctcg agatactcga actgtccgct
     4261 ccagaccacg taggagcctg ccgggaacgc aatgctggcg ttcaccgcat tctgggcgtc
     4321 ggcgacgtaa ccgccgagat cgcgatcgcg gatgtcgaca tagatgtaga cggcgagctg
     4381 accgttttcg gtgcggatcg atgttggacc gcgcgacggc gcgatcttcg ccacctctcc
     4441 caacggcacc gcgccgccat tgggcatcgg gaccagaaca tcgctggcga tggcttgcgc
     4501 gttacttcga agatcacggg gatagcgcat gttgacgctg aagcgctggc ggccctctac
     4561 cgtggtggtc acggtctggc cgccgagcgc ggtagcgatg acgtcctgga cgtcctgaac
     4621 catgatgcca tagcgggcca atgccgcgcg gtccggcgtc acatcgagat aatagccgcc
     4681 gagcgcgcgc tccgcgtagg ccgacgacgt tcccggcacg gttttgagaa cctgctcgat
     4741 ctgtttcgcc agcctgtcga tctcgacgag gtccgtgccg atcaccttgc ccccgaccgg
     4801 cgtgcgaatc ccggtcgaca gcatgtcgat gcgcgccctg atcggcatgg tccaggcgtt
     4861 ggaaacgccc gggaattgca aggcacggtc catttcggcg gtcagactct cgatcgtgac
     4921 gcccggccgc cattcctgct tcggcttcag attgacgatg gtctcgaaca tctccgaagg
     4981 tgccggatcg gtggcggtgg aggcacggcc ggccttgccg tacaccgagg cgacttcctg
     5041 gaacgaccgg atgatcttgt cctgtgtctg caacagttcg gcggccttgg tcacggaaat
     5101 gccgggcaag gttgtcggca tgtaaagcag cgtgccttca ttcaggctcg gcatgaactc
     5161 gctgcccaat tggcgcgcgg gccagatcgt gacggcgaga acagccagcg agatgagaat
     5221 caccagcacc ttggcccgca tcacacaagc gatcaccggc cgatagatcc agatcaggaa
     5281 acggttgatc ggattcctgt gctccgggac gatccggccg cggatgcaga tgaccatcag
     5341 cgccggcacc agcgtcaccg acagcaaggc ggccgccgcc atcgaaaagg tcttggtgaa
     5401 cgccaacggg ctgaacagcc gtccctcctg cgattccagg gtgaagatcg gcatgaacga
     5461 gacggtgatg atcagcaggc tgaaaaacaa cgccggcccg acttcgccgg ccgcctcgat
     5521 caggacatcg acccgcgatc gtccggggtc ggcgcgttca agatgtttat gggcattttc
     5581 gatcatcacg atggcggcgt cgatcatggc gccgacggca atggcgatgc cgcc
//
LOCUS       DATNOI010000487        13606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2491330,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000487 DATNOI010000000
VERSION     DATNOI010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13606
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2491330"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2134
                     /locus_tag="VN968_19460"
     CDS             <1..2134
                     /locus_tag="VN968_19460"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL31448.1"
                     /translation="RIADPAPVEEIRVRTFDTFQRIDPRVKTARPVTIVDIDEASLAK
                     LGQFPWPRTRIADLITGLTKLGAVVIAFDIVFSEPDRLNPDVVADTFRDLDEETRSRL
                     RALPSNDQILADAMRRSRVVLGESGLPQVQSEFDKSLPLTGLAMLGEEPQPFMLEWPG
                     LLRNVPLLEAAAAGRGLFTIWPERDGIVRRVPVMMLAQGATMPSLSFEMLRVATGTDT
                     IFIKSDQAGIKSIGVKGFQIPTDRNGQLWVHFAHHDPKILVSALDVIEGKVAPEKIKG
                     QLVLIGTSAVGLNDNKTTPVDPSMPGVEIHAQVIESALTRAVLTQPNYGIAVELCAAI
                     LFGILVIAFAPLFGPVTLVAVGALFATLLIGTSWYFYTQHRLLIDFTYPLLSTTSIYL
                     TLIFTSFVREQRQRKQIRTAFAQYMSPTLVEQLAQSPEKLVLGGEEREMTIMFSDVRG
                     FTTISESYKNDPQGLTALMNRFLTPLTNAILERKGYIDKYMGDAIMAFWNAPLDDREH
                     QVNACEAAVDMLERIDVLNKQREFEAKEGGHVYMPLNVGVGLNTGTCVVGNMGSDLRF
                     DYSVLGDSVNLASRLEGQSKEYGFPIIVGSRTALAAREKFAILELDFIMVKGKKEPEV
                     IYAIAGRADTAHSARFQRLRNLTIEMLACYRNRDWEGALAAIERGRRTDEARSLELLY
                     NLYEARIRGYQENPPPEDWNGAFALLTK"
     gene            complement(2281..3888)
                     /locus_tag="VN968_19465"
     CDS             complement(2281..3888)
                     /locus_tag="VN968_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31449.1"
                     /translation="MRTRNSILLAALAVAIGAVLPALPAQAEQVVRYGISMADIPLTT
                     GQPDRGAGAYQFTAYTIYDPLVAWEMDVSDRPGKLVPGLATEWKVDDKDKTKWRFTLR
                     QGVKFHDGSDFNADAVIWNLDKVLNDKTPQFDKRQSAQVKTRLPSVASYAKIDDSTIE
                     ITTKTVDSFFPYQMLWFLVSSPAQYEKLGKDWDKFASQPSGTGPFKLTKLVPRELAEL
                     SKNEDYWNKKRIPKVDKLILIPMPEALTRTNALLAGQVDLIETPAPDAVPQLKSAGMR
                     IVDNITPHVWNYHLSVLPGSPWTDVRLRKALNLAIDRDAVVGLMNGLAKPAKGQVDPS
                     SPWFGKPTFELKYDVAAAKKLVEEAGYSKQKPLKATFIIAQGGTGQMLSLPMNEFLQQ
                     SFKEIGVDVDFKVVELETLYTHWRKGAADEMNAGITSNNIAYVTSDPLYAIVRFFHSG
                     QIAPVGVNWGGYKNPKVDALIDEAKQTFDSTKQDELLAQAHSLIVDDAVLVWVVHDTN
                     PHALSPKIKKFVQAQHWFQDLTTIGVE"
     gene            4143..5765
                     /locus_tag="VN968_19470"
     CDS             4143..5765
                     /locus_tag="VN968_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL31450.1"
                     /translation="MMLAMACAAGLPRIAGAETVLRIGMTAADIPRTLGQPDQGFEGN
                     RFTGLTMYDALTMWDLSAADKPSVMIPGLATEWAVDDADKKKWTFKLRPGVTFHDGSP
                     FNADAVVWNVEKVLKQDAPQFDPSQVGVTASRMPTLASARKIDDMTVELTTKEPDSFL
                     PINLTNLFMASPAKWQKLFDAAEGADAKAKSQAAWAAFARAASGTGPWKLSSFTPRER
                     LELTKNENYWDKARVPKVDKMLLLPMPEANARTAALLSGQVDWIEAPAPDAVKEIKQR
                     GFVLQTNEEPHVWPWQFSRIEGSPWNDIRVRKAANLCVDREGLKEGLLAGLMVPATGT
                     FEPGHPWRGKPTFEIKYDKPAAQKLMQEAGFGPAKKLSVKIQTSASGSGQMLPLPMNE
                     YLQQALAECYFDVQLDVIEWNTLFTNWRRGVKDPSANGANATNVTYAAMDPFFAMVRF
                     LQSSMAPPVSNNWGFINNPKFDELVTKARQTFDPAARDTALAELHAASVDDAAFLYVA
                     HDVAPRAMSPKIKGFVQPKSWFVDFSPIAMVP"
     gene            5943..6896
                     /locus_tag="VN968_19475"
     CDS             5943..6896
                     /locus_tag="VN968_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31451.1"
                     /translation="MLVYIARRIVYVIPIVISVALVCFLLVHITPGDPLVAILPADAS
                     QELAAQLRVAYGFDRPLPVQFGLWLWKAVNGDLGHSIATGRPVLSEVMRAVSNTVTLA
                     IAAASIGFTLGLFFGLIAGYFRDTWIDKVATSIAIAGVSVPHYWLGMVLVIIFSVQLN
                     WLPAVGAGPGGSGAWGWDWEHIRYLILPAITTSVIPMGIVTRTVRALTGDILSQDFVE
                     ALRAKGLRETRVFRHVIKNAAPTAMAVMGLQLGYMLGGSILIETVFSWPGSGLLLNSA
                     IFQRDLPLLQGTILVLALFFVFLNLMVDIAQAAIDPRIKRS"
     gene            6916..7803
                     /locus_tag="VN968_19480"
     CDS             6916..7803
                     /locus_tag="VN968_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31452.1"
                     /translation="MSAITDAALQAAPADKARGYWTTVGRRITRDKVSMVCAFILALI
                     FLSALCAPWLGLADPYQGSMIRRLRHIGTPNYPLGTDELGRDMLARLIYGGRLSLVVG
                     ILPVIFAFVIGTSLGLVAGYVGGKINTAIMRTIDVFYAFPSVLLAIAISGALGAGIVN
                     SIVSLTVVFVPQITRVAESVTTGVRNMDFVEAARASGAGPFTIMRVHMLGNVLGPIFV
                     YATGLISVSMILAAGLSFLGLGTKPPEPEWGLMLNTLRTAIYINPWVAALPGAMIFAV
                     SICFNLLSDGMRSAMDIRN"
     gene            7808..8875
                     /locus_tag="VN968_19485"
     CDS             7808..8875
                     /locus_tag="VN968_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31453.1"
                     /translation="MNEISPQVELLEPIEDRGGAAQPLLQVNGLTKHFPVRGDLFAKR
                     KTVRAVDDVSFDIAKGETVGIVGESGCGKSTTARLLMHLMKRDAGDIIYDGMSVGRTL
                     SLRELRRAMQMVFQDSYASLNPRLTIEESIAFGPKVHGMADTAARALARELLGKVGLR
                     PELFANRYPHEISGGQRQRVNIARALALSPRLVILDEAVSALDKSVEAQVLNLLVDLK
                     REFGLTYLFISHDLNVVRYISDRVLVMYLGEVVELGPVDRVWDAPAHPYTRALLAAMP
                     SSDPDNRTETPPISGDPPNPIDPPSGCRFHTRCPFAEPLCANATPKLTALDKLGHQAA
                     CYMAIPGSGHSLAPAKGADLA"
     gene            8872..9069
                     /locus_tag="VN968_19490"
     CDS             8872..9069
                     /locus_tag="VN968_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31454.1"
                     /translation="MTKPSPKEIKAMTDVAGLPVSADVAARIAGSIGPAFEGFAAVAG
                     TLPFDLEPASFLVVQAAKVLK"
     gene            9066..10478
                     /locus_tag="VN968_19495"
     CDS             9066..10478
                     /locus_tag="VN968_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXL31455.1"
                     /translation="MSTEPALLSLTEVAQAIAQKRFSSREVTLACLNRVAQWQPRLNA
                     FMAIETDEALAAADAADAALAKGSNYGALHGVPLAHKDMYYEAGKIVTCGSHIRRDFV
                     ATTTSTALQRLKDAGTIRLGSLQMAEFAYGPTGHNTHYGPVHNPWDVDRVTGGSSSGS
                     GAAVAARLTFAALGSDTGGSIRMPAHFCGVTGLKTTVGRISRAGAMPLSQSLDTVGPL
                     ARTALDCALLLGLMAGADADDPTAVGGPLPDYLAATRKSIKGLKIGVPTAFYVDDLDP
                     EVERILDDTITVLKREGAEIVQVELPDQRQLTAACQLVLAVEAAAFHKRWMIERPQDY
                     GPQVLMRLQNGLAIPGVSYLEAMRWRGPALAAYLAAVAGVDAVIAPVAPVAAPTIAES
                     DVGNSPDAEAVIQRLTRFTRPINYLGLPALAIPSGFTKSGLPVGMQLIGRSFDEAMLL
                     RIGAALQRATDFHARVPKPA"
     gene            10475..11458
                     /locus_tag="VN968_19500"
     CDS             10475..11458
                     /locus_tag="VN968_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31456.1"
                     /translation="MSNLVEIRDLNIRFTGERTVHAVNDLSLVLGEGEVLGLLGESGS
                     GKSVTLRALMRLLPKKRTQISGSVEVLGRDVLALDDEELSAFRGQTVSMIFQEPALAL
                     DPVYTIGHQIAETVMRHEGKSQAEATARALEMLEVVRIPSAKRRLDAYPHEMSGGMRQ
                     RAMIALALACKPKILLADEPTTALDATVQIQILLLLRELQREFGMSVIFVTHDIGVAI
                     EICDRVAVMYAGQIVEQGTLSQIVRSPVHPYARGLLASTVHGAKRGQRLETIPGTPPS
                     LDRAPSNCSFAPRCGFVQPRCVEQLPPNVQMVANRAARCILAERAGIVAAT"
     gene            complement(11484..11732)
                     /locus_tag="VN968_19505"
     CDS             complement(11484..11732)
                     /locus_tag="VN968_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31457.1"
                     /translation="MTQPDIWYVAYGPDKTVKTDDSASGVMRTTRTFKTEVDAKLFAM
                     QILAKGWTVSAGTLNPHLPKQIVGPSQIERWADPGLGG"
     gene            11890..12159
                     /locus_tag="VN968_19510"
     CDS             11890..12159
                     /locus_tag="VN968_19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HXL31458.1"
                     /translation="MRMSNESILVILFVGLIAGWLAGQIVRGTGFGIIGDILVGIAGA
                     LVASLLFPRLGIAIGTGLVREILYSAIGAIILLVVVRLLRSGGRL"
     gene            complement(12175..12591)
                     /locus_tag="VN968_19515"
     CDS             complement(12175..12591)
                     /locus_tag="VN968_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HXL31459.1"
                     /translation="MIRRFIGIAALVAAVALPVVAQAQGVPGGIERGSRDGERAAGAV
                     GAVVGGVIGGVLGGVAGVLGVEERPRFRNYAAEQHRPSYQYREDVRVGGVLPDEGVSY
                     YDVPPEYGARDYRYTVVNGRTVLVDPRTNRIVEVVE"
     gene            complement(12712..13140)
                     /locus_tag="VN968_19520"
     CDS             complement(12712..13140)
                     /locus_tag="VN968_19520"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXL31460.1"
                     /translation="MGSEPLVEVTGLPVCAPALIKSGLRKPKDLSRQVLIHLTSQPRA
                     WPAWLNEAGIAELTPRGHLWLDSVPAMLEAAEQGLGVTLAMAPLIKARPGFGKKLVAP
                     FDVEARHTETIYLVSRTEQARDRRIGALRRWIIDAVGRIS"
     gene            13112..13336
                     /locus_tag="VN968_19525"
     CDS             13112..13336
                     /locus_tag="VN968_19525"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31461.1"
                     /translation="MTSTSGSDPNSNLTVQLFRPPSGEWVGVRAETRWRPATGCGVGS
                     GTLLDTRGEVGRVSMSVVLVPFPKAAAAAS"
BASE COUNT         2434 a         4223 c         4308 g         2641 t
ORIGIN      
        1 gcgcatcgcc gatcccgccc ccgtcgagga gattcgcgtc agaaccttcg ataccttcca
       61 gcgcatcgac ccgcgcgtga agacggcaag gccggtcacc attgtcgata tcgacgaagc
      121 gagcctggcg aaactcgggc agtttccctg gccgcggacc cggatcgcgg atctgattac
      181 tggtctgacc aagctcggcg ccgtcgtgat cgcgttcgac atcgtattct ccgagcccga
      241 ccggctcaat ccggacgtcg tggccgacac gtttcgcgac ctcgacgagg aaacccgaag
      301 caggctgcgg gcgctgccga gcaacgatca gatcctcgcc gacgccatgc ggcgttcgcg
      361 cgtggtgctc ggcgaatccg gactgcctca ggtccagtcg gaattcgaca agtcgcttcc
      421 gttgacgggc ctcgccatgc tcggcgagga gcctcagccc ttcatgctgg agtggccggg
      481 tttgctccgg aatgttccgc ttctcgaagc cgcggctgcg gggcggggcc tgttcacgat
      541 ctggcccgaa cgcgatggca ttgtccgccg cgtgccggtg atgatgctgg cccagggcgc
      601 gaccatgccg tcgctgagct tcgaaatgtt gcgggtcgcg accggcaccg atacgatttt
      661 catcaaatcc gatcaggccg gcatcaagag catcggcgtc aagggtttcc aaattccgac
      721 cgatcgcaac ggccagctct gggttcattt tgcgcatcac gatcccaaga ttttagtctc
      781 ggcgctcgac gtgatcgagg ggaaggttgc gcccgaaaag atcaagggcc agctggtgct
      841 gatcggaaca tcagccgtgg ggcttaacga taacaagacc acgccggtcg atccttcgat
      901 gcccggtgtc gaaatccacg cccaggtgat cgagagcgcg ctgacacgtg cggtgcttac
      961 gcagccgaat tacggcatcg ctgtcgaact ctgcgcggca atcctgtttg gcattctggt
     1021 catcgcgttt gcgccgctat tcggaccggt caccctggtt gccgtcggcg cgctgttcgc
     1081 aacgctgctg atcggaacat cctggtattt ttacacccag catcggctgc tgatcgattt
     1141 tacctatccc ttgctgtcga ccacctcgat ctatctcacc ctcattttca ccagcttcgt
     1201 cagggagcag cggcagcgga aacagattcg tacggcgttc gctcagtata tgtcgccgac
     1261 gctggtcgag cagctggcgc aatcgccgga aaagctcgtg ctcggcggcg aggagcgcga
     1321 gatgaccatc atgttctccg acgtccgggg cttcaccacg atctcggagt cctacaagaa
     1381 cgatccgcag gggttgacgg cgctgatgaa ccggttcctg acgccgctga ccaacgcgat
     1441 cctggagcga aagggttata tcgacaaata catgggcgac gcgatcatgg cgttctggaa
     1501 tgcgccgctc gacgacaggg agcatcaggt caacgcctgc gaagccgcgg tcgacatgct
     1561 ggagcggatc gatgtgctca acaagcagcg cgagttcgag gcgaaagagg gcggtcacgt
     1621 ctacatgccg ctcaacgtcg gcgtcggact gaataccggc acttgcgtgg tcggcaatat
     1681 ggggtccgac ctgcgtttcg attattcggt gctgggcgac agcgtcaacc tcgcatcgcg
     1741 gctggagggg caatccaagg agtatggctt cccgatcatc gtcggctcca ggaccgcgct
     1801 cgcggccagg gagaagtttg cgattctcga actcgatttt atcatggtga agggcaagaa
     1861 agagcccgag gtgatctatg cgattgcggg gcgcgcggac accgcgcatt ccgcccgctt
     1921 ccagcgattg cgcaacctga ccatcgagat gctggcgtgt tatcgcaacc gcgactggga
     1981 aggcgcgctg gcggcgatcg agcggggccg ccgcaccgat gaggcccgtt ccctcgagtt
     2041 actctataat ttgtacgagg cgcgcatccg cggctatcag gaaaacccgc ccccggaaga
     2101 ctggaacggc gctttcgcgc tgttgaccaa gtagcggatt gcaagatctg cgtcgtcccg
     2161 gcgaacgccg ggtcccataa cccccggcgt gcatcgttgc agagggcatc tgcttcagta
     2221 ccctatcgcg aggacacgga gtatgggtcc cggcctccgc cgggacgaca gcctgatttt
     2281 ttactccacc ccgatcgtcg tcagatcctg aaaccagtgc tgcgcctgca cgaacttctt
     2341 gatcttgggc gacagcgcgt gcggattggt gtcgtgcacc acccacacca gcaccgcgtc
     2401 gtcgacgatc agcgaatgcg cctgcgccag tagttcgtcc tgcttggtgg aatcgaacgt
     2461 ctgcttggct tcgtcgatca aggcatcgac tttcggattc ttgtagccgc cccagttgac
     2521 gccgaccggc gcgatctggc cggagtggaa gaaacggacg atggcgtaga gcggatccga
     2581 cgtcacatag gcgatgttgt tcgaggtgat gccggcattc atttcgtccg cggcgccttt
     2641 gcgccagtgc gtgtaaagcg tttccagttc gaccaccttg aagtcgacat cgacgccgat
     2701 ttccttgaag ctttgctgca ggaattcgtt catcggcagc gacagcattt gcccggtgcc
     2761 gccttgcgcg atgatgaagg tggccttcag cggcttttgt ttggaatagc cggcttcctc
     2821 gacgagcttc ttcgcggccg caacgtcgta tttgagttcg aaggtcggtt tgccgaacca
     2881 ggggctcgat ggatcgacct gacccttggc aggtttggca aggccgttca tcagtccgac
     2941 caccgcgtcg cgatcgatcg cgagattgag cgccttgcgc agccggacgt cggtccaggg
     3001 cgagcccggc agcacgctga gatgataatt ccaaacatgc ggggtgatgt tatcgacgat
     3061 ccgcatcccc gccgatttga gttgcggcac cgcatcgggg gccggggttt cgatcagatc
     3121 gacctgcccc gccaacagcg cgttggttcg cgtcagcgct tccggcatcg gaatcaggat
     3181 cagcttgtcg accttcggaa tccgcttctt gttccagtaa tcctcgttct tcgaaagttc
     3241 ggcgagttcg cgcggcacga gttttgtcaa tttgaacggc ccggtgcctg agggctggct
     3301 tgcgaacttg tcccagtcct tgccgagctt ttcgtattgc gcggggctcg agaccaggaa
     3361 ccagagcatc tggtagggaa agaacgagtc gacggtcttg gtggtgattt ctatcgttga
     3421 atcgtcgatc ttggcatagc tcgccaccga gggcaggcgg gtcttcacct gcgcgctctg
     3481 acgcttgtcg aattgcggcg tcttgtcgtt caggaccttg tcgagattcc agatcaccgc
     3541 atcggcgttg aagtcgctgc cgtcatggaa tttgacgccc tgccgcagcg taaatcgcca
     3601 tttggtcttg tccttgtcat cgaccttcca ttcggtggcg aggcccggca ccagtttgcc
     3661 cggccggtcc gacacatcca tttcccaggc gaccagcgga tcgtaaatcg tataggcggt
     3721 gaactgatag gcgccggcgc cacgatccgg ctggccggtt gtcagcggaa tgtccgccat
     3781 cgaaatgccg tatcgcacca cctgttcggc ttgcgcggga agggcgggca ggacagcgcc
     3841 gatggccact gccaaggccg caagaaggat cgaatttcgg gtgcgcatga actaagctcc
     3901 attggatgag cgattggaat aatcgatacc agcgtgcaag gttgatgcca gattaggcag
     3961 ttgtgtcttt tgctggcctg ccatcacaag gagttgtgcc atcaatagca attcggcggt
     4021 acccgcatca ttggcacagc cgttgcatac cgtttgcata agaaatagtc actgaaagtc
     4081 gaaaagggat ttccacgatg cgtaccgaaa aatctgcgaa aggccgggca agcgcggcag
     4141 tcatgatgct ggcgatggca tgcgcagcag gactgccgcg gatcgccggc gccgaaaccg
     4201 ttctgcgcat cggcatgacc gcggccgaca ttccgcgcac gctgggccag cccgatcaag
     4261 gttttgaggg caatcgcttt accggcctca ccatgtatga cgcgctgacg atgtgggacc
     4321 tctccgccgc cgacaagccg agcgtcatga ttcccgggct tgcgaccgaa tgggccgtcg
     4381 atgacgctga caagaagaag tggacgttca agctgcgtcc cggcgtcacc ttccacgacg
     4441 gctcgccgtt caatgccgat gctgtagtct ggaacgtcga aaaagtgctc aagcaggatg
     4501 cgccgcaatt cgatccgagc caggtcggcg tgaccgcgtc gcggatgccg acgctggctt
     4561 cggcccgcaa gatcgacgac atgacggtgg aattgaccac caaggagccg gatagcttcc
     4621 tgccgatcaa cctcaccaac ctgttcatgg cgagccccgc gaaatggcag aagctgttcg
     4681 atgccgccga aggcgcggat gccaaggcga aatcgcaagc cgcctgggcc gcgttcgcgc
     4741 gtgccgcgtc cggcaccggg ccgtggaagt tgtcgagctt cacgccgcgc gagcggctgg
     4801 agctgacgaa gaacgagaat tattgggaca aggcgcgcgt ccccaaggtc gacaagatgc
     4861 tgttgctgcc gatgcccgag gcaaatgcgc gcaccgcggc actactctcc ggccaggtcg
     4921 actggatcga agcgcccgcg ccggatgcgg ttaaggaaat caagcagcgc ggcttcgtgc
     4981 ttcagaccaa tgaggagccc catgtctggc cctggcagtt ctcgcgcatc gaaggttcgc
     5041 catggaacga cattcgcgtt cgcaaggcgg ccaatctctg cgtcgaccgc gagggcctga
     5101 aggagggcct gctcgccggc ctgatggtgc cggcgaccgg caccttcgaa ccgggccatc
     5161 cctggcgcgg caagccgacc ttcgaaatca agtatgacaa gcccgcggcg cagaagctga
     5221 tgcaggaggc cggcttcggc ccagctaaga aattgtcggt caagatccag acctcggcgt
     5281 cgggatccgg ccagatgctg ccgttgccga tgaacgagta tctgcagcag gcgctggcgg
     5341 aatgttattt cgacgtccag ctcgatgtga tcgaatggaa tacgctgttc accaactggc
     5401 ggcgcggcgt caaggatccc tccgccaacg gcgccaacgc caccaacgtg acctatgcgg
     5461 cgatggatcc gttcttcgcc atggtgcgtt tcctgcaatc gtcgatggcg ccgccggtgt
     5521 cgaataattg gggctttatc aataatccga aattcgacga actggtcacc aaggcacgtc
     5581 agactttcga tccggcggcg cgtgacaccg cgctggccga attgcatgcg gcctcggtcg
     5641 acgacgcggc gttcctgtat gtcgcccacg acgtcgcgcc gcgcgcgatg agccccaaga
     5701 tcaaaggctt cgtgcagccg aagagctggt tcgtggactt ctcgccgatt gccatggtgc
     5761 cgtagcgggc ggacaccacg aacgctcgct gcgtcgtccc ttttccctcc ccccttgcgg
     5821 gggagggtca gggagggggt aagccacaag cgctgaactc gcggctaccc ccacccccga
     5881 cccctccccg caagggggag gggagggcca ctgccgaatt cgacaatctg taaggttcct
     5941 acgtgctcgt ctatatcgcc cggcgcatcg tctatgtgat tccgatcgtc atcagcgtgg
     6001 cgctggtgtg ctttctgctg gtgcacatca cgcccggcga tccgctggtg gcgatcctgc
     6061 ccgcagatgc gtcgcaggaa ctggcggcgc agttgcgcgt cgcctacggc ttcgatcgtc
     6121 cgctgccggt gcagttcggc ttgtggctat ggaaggcggt caatggcgat ctcggccatt
     6181 ccatcgccac cggacgcccg gtgctgtcgg aagtcatgcg cgcggtcagc aataccgtca
     6241 ccctggcaat cgcggcggca tcgatcggct tcacgcttgg attattcttc ggcctgatcg
     6301 ccggctattt ccgcgatacc tggatcgaca aggtggcgac ctcgatcgcg atcgccggcg
     6361 tctcggtgcc gcattactgg ctcggcatgg tgctggtgat catcttctcg gtgcagctta
     6421 actggctgcc cgcggtcggc gccggccccg gcggctccgg cgcatgggga tgggactggg
     6481 aacacatccg ctatctgatc ctgcccgcga tcacgacttc ggtgattcca atggggattg
     6541 tcacccgcac cgtgcgtgcg ctgaccggcg atatactgag ccaggatttc gtcgaggcgt
     6601 tgcgcgccaa gggtttgcgc gaaacccgcg tgttccgtca tgtcatcaag aacgcagcac
     6661 caacggcgat ggcggtgatg ggactgcaat tgggatacat gctcggcggt tcgatcctga
     6721 tcgaaaccgt gttctcgtgg ccgggctcgg ggctgttgct caattccgcg atcttccagc
     6781 gcgatctgcc gctgctgcag ggcacgatcc tggtgctggc gctgttcttc gtgttcctca
     6841 atctgatggt cgatatcgcg caggccgcga tcgaccctcg tatcaagcgg agctgagcga
     6901 agtgagtatc agcccatgag cgcgataaca gatgcagcct tgcaggccgc gcccgccgac
     6961 aaggcgcgtg gttactggac cacggtcggg cgccgcatca cgcgcgacaa agtcagcatg
     7021 gtctgcgcgt ttattctggc gctgattttt ctctccgcgc tgtgcgcgcc gtggctcggc
     7081 ctcgccgatc cctatcaggg ttcgatgatc cgccggctgc gccatatcgg tacgccaaac
     7141 tatccgctcg gcaccgacga actgggccgc gatatgctgg cgcggctgat ctatggcggg
     7201 cgcctgtcgc tggtggtcgg cattctcccc gtcatctttg ccttcgtgat cgggacctcg
     7261 ctcggcctcg tcgccggcta tgtcggcggc aagatcaaca ccgcgatcat gcgcaccatt
     7321 gacgtgttct acgcctttcc ttcggtgctg ctggcgatcg cgatttcggg agcgctgggc
     7381 gcgggcatcg tcaattcgat cgtctcattg acggtggtct tcgtgccgca gatcacccgg
     7441 gtggccgaga gcgtcaccac cggcgtgcgc aacatggatt tcgtcgaggc ggcgcgggcc
     7501 tccggcgccg gccccttcac catcatgcgc gtgcacatgc tcggcaacgt gctgggcccg
     7561 attttcgttt acgccaccgg gctgatctcg gtgtcgatga tcctggccgc ggggctgtcg
     7621 ttcctcgggc tcggcaccaa accgccggag ccggaatggg gtttgatgct caacacactg
     7681 cgcaccgcga tctatatcaa tccatgggtg gcggccttgc ccggcgccat gatcttcgcg
     7741 gtgtcgatct gcttcaacct gctcagcgat ggcatgcgca gcgccatgga catccggaac
     7801 tgacgtcatg aacgagatca gcccacaggt cgaactgctt gagccgatcg aggatcgcgg
     7861 cggcgcggcg cagccgttgc tgcaggtcaa cggactgaca aaacattttc cggtgcgcgg
     7921 cgacctgttt gcgaaacgca agacggttcg cgcggtcgat gacgtgtcgt tcgacatcgc
     7981 caagggcgaa accgtcggga tcgtcggcga atccggctgc ggcaagtcga ccaccgcgcg
     8041 gctcctgatg cacctgatga agcgcgatgc cggcgatatc atctatgacg gcatgtcggt
     8101 ggggcgcact ttgtcgcttc gcgaacttcg ccgcgccatg cagatggtgt ttcaggacag
     8161 ctatgcctcg ctcaatccgc gcctcacaat cgaagaatcg atcgccttcg gtccgaaggt
     8221 ccacggcatg gccgacacgg ccgcgcgcgc attggcgcgc gagctgctcg gcaaggtggg
     8281 cttgcggccg gaactcttcg ccaaccgcta tccgcacgag atttcaggcg gccagcgtca
     8341 gcgcgtcaat atcgcccgcg cactggcctt gtcaccgcgt ctggtgatcc tcgacgaagc
     8401 ggtgtcggcg ctggacaagt cggtcgaggc gcaggtgctc aacctgctgg tcgatctcaa
     8461 gcgggaattc gggctgacgt atctcttcat cagccacgat ctcaacgtgg tgcgctacat
     8521 ttccgaccgc gtgctggtga tgtatctcgg cgaagtggtc gagcttggtc cggtggaccg
     8581 ggtgtgggac gcgccggcgc atccctatac gcgggcgttg ctggcggcga tgccgtcgtc
     8641 cgatcccgat aaccggacag aaacgccgcc gatctcgggc gatccgccca acccgatcga
     8701 tccgccctcg gggtgccggt ttcacacccg ttgtccgttc gcggagccgc tttgcgcaaa
     8761 tgccacacca aaacttaccg cactcgataa actgggtcat caggccgcgt gctacatggc
     8821 gatccccggt tccggtcaca gcctcgcgcc agccaaagga gcagatctcg catgacgaaa
     8881 cccagcccca aggagatcaa agcgatgact gatgtcgcgg gtttgccggt gagcgccgat
     8941 gtcgccgcgc gcatcgccgg ctccatcggc cccgccttcg aaggttttgc cgcggtcgcc
     9001 ggcacgttgc cgttcgatct ggagcctgcg agcttccttg tcgtgcaagc tgcgaaagtt
     9061 ttgaaatgag caccgagccc gcattgctat cgctgacaga ggtcgcgcag gccatcgcgc
     9121 aaaagcgctt ctcgtcgcgt gaggtgacgc tagcctgcct taacagggtc gcgcagtggc
     9181 agccccgtct caatgccttc atggcgatcg aaaccgacga ggcgctggcg gcagccgatg
     9241 ccgccgacgc ggcgcttgcc aaaggcagca actacggcgc gctgcacggt gtgccgctgg
     9301 cgcacaagga catgtattac gaggccggca agatcgtgac gtgtggatcg catatccggc
     9361 gtgatttcgt cgccaccacg acttcgaccg ccttgcagcg tctgaaggac gccggcacga
     9421 ttcgcctcgg ctcgttgcag atggcggagt tcgcctacgg gccgaccggc cacaacacgc
     9481 attacgggcc ggtgcataat ccctgggatg tcgatcgcgt caccggcggt tcgtcgtcgg
     9541 gatccggcgc cgcggtcgcc gcacgtctga cgtttgcggc attggggtcg gacaccggcg
     9601 gctcgatccg gatgccggcg catttctgcg gcgttaccgg attgaagacc acggtcggcc
     9661 gcatcagccg cgccggcgcg atgccgctgt cgcagtcgct cgacaccgtc ggaccgctgg
     9721 cgcgcacggc gctggattgt gcgttgctgc tgggcctgat ggcgggcgcg gacgccgatg
     9781 acccaaccgc ggtcggaggc ccgttgccgg attacctggc cgcgacacgg aaatcgatca
     9841 aaggcctgaa aatcggcgtg cccaccgcgt tctatgtcga cgacctcgat cccgaagtcg
     9901 agcgaatcct tgacgacacg atcaccgtgc tcaagcgtga gggcgcggaa atcgttcagg
     9961 tcgaactgcc ggaccagcgt cagctcacgg ccgcatgtca gctggtgctc gcggtcgaag
    10021 ccgcggcgtt tcacaagcga tggatgatcg agcgcccgca ggattacggc ccgcaggttc
    10081 tgatgcgatt gcaaaatgga ctggccatcc ccggcgtgtc ctatctcgaa gcgatgcgct
    10141 ggcgcgggcc ggcgctggct gcctacctcg cggcggtcgc cggcgtcgat gccgtgatcg
    10201 cgccggtggc gccggtcgcg gcaccgacca ttgccgagag tgacgtcggc aacagccccg
    10261 acgccgaagc ggtgatccag cggctgacgc ggttcacccg cccgatcaat tatctcgggc
    10321 tgccggcgct ggcgattcca tcgggtttta caaaaagcgg tcttccggtc ggaatgcaat
    10381 tgatcggccg ttcgttcgac gaagccatgc tgttgcggat tggcgccgcg ttgcagcgcg
    10441 caaccgattt ccacgcccgg gtgccgaaac cagcatgagc aatctggtcg aaatccgcga
    10501 tctcaacatt cgctttaccg gcgagcgcac cgtgcatgcc gtcaacgatc tcagccttgt
    10561 cctcggcgag ggcgaggtgc tcggcctgct cggcgagtcc ggctccggca agagcgtcac
    10621 cttgcgcgcg ctgatgcggc tgttgccgaa gaagcgcacg cagatctcgg gaagcgtcga
    10681 ggtgttgggg cgcgacgtgc tggcgctcga tgacgaggag ctgtcggcat tccgcggcca
    10741 gaccgtatcg atgatcttcc aggagccggc gctggcgctc gatccggttt acaccatcgg
    10801 ccaccagatc gcggaaaccg taatgcgtca cgagggcaag agccaggccg aggcaacggc
    10861 gcgcgcgctg gaaatgctgg aagtggtgcg gattccctcg gccaagcgcc ggctcgatgc
    10921 ctatccgcac gagatgtccg gcggcatgcg gcagcgcgcg atgatcgcgc tggcgctggc
    10981 ctgcaagccg aagattctgc tggcggacga gccgaccacg gcgctcgatg ccaccgtgca
    11041 aatccagatc ctgctgctgc tgcgcgaact gcagcgcgag ttcgggatgt cggttatttt
    11101 cgtcacccac gatatcgggg tcgcgatcga aatctgcgat cgcgtcgcag tgatgtatgc
    11161 cgggcagatc gtcgagcagg gcacgctgag ccagatcgtg cgttcaccgg tacatcctta
    11221 cgcgcgcggt ttgctggcct ccaccgtgca cggcgccaaa cgcggccagc ggctggaaac
    11281 cataccggga acgccgccct cgctcgacag ggcgccgagc aactgctcgt tcgcgccgcg
    11341 ctgcggtttc gtccagccgc gctgcgtcga gcaactgccg cctaatgtgc agatggtagc
    11401 gaacagggcc gcaaggtgca tcctggccga acgcgccggg atcgtcgctg cgacgtaatc
    11461 gcaagagaca gcaagataag ccttcagccg ccgagacccg ggtcggccca gcgttcgatc
    11521 tgggagggcc cgacaatctg cttgggcaga tgagggttga gcgtgcccgc gctgacggtc
    11581 cagcctttcg ccagaatctg catggcgaac agcttggcat cgacttccgt cttgaaggtc
    11641 cgggtcgtgc gcatcacgcc cgacgcgctg tcgtcggtct tcacggtttt gtccggccca
    11701 taggccacat accagatgtc gggttgtgtc attcctgcat aacgcgaaac acgagtgaaa
    11761 gtttcatttg gtgtcggagg ctcgcgcgta ttttgcgaag cgcccagatt ggaatggtca
    11821 cgtcaggcaa ttgtcgatag gttcgaaagc agcggggaga aatgacgtaa ccaacggggg
    11881 gaaatcttta tgcgcatgtc caatgaaagc attctcgtta ttctgttcgt cggcctgatt
    11941 gcaggctggc tggcggggca aatcgtacgc ggcaccggct ttggcatcat tggcgatatt
    12001 ctcgtcggta tcgccggcgc gctggtcgcc agcctgttgt ttccaaggct cggcattgcc
    12061 atcggtaccg gcctcgtgcg cgagatcctt tattccgcga tcggcgccat cattctgttg
    12121 gtggtcgtgc ggctgctccg aagcggcggc cggctctgac gatgcagagc gatgttactc
    12181 gacgacctcg acaatgcggt tggtccgcgg atcgaccagc acggtgcggc cgttcacaac
    12241 cgtgtaacgg taatcccggg caccatattc cggcggcacg tcgtagtagc tgacgccctc
    12301 atcgggcagc accccgccaa ctcgcacgtc ctcacgatat tgataggaag ggcggtgctg
    12361 ctcggctgcg taattgcgaa agcgcggacg ctcctcgacg ccgagaaccc cggcaacgcc
    12421 accgagcacc ccgccaatga caccgccgac cacggcgccg acagcaccgg cggccctttc
    12481 gccgtctctt gagcctcgct caattccacc gggaaccccc tgggcctgcg ccacaacagg
    12541 gagcgccact gcggcgacaa gggcggcaat tccgataaag cggcggatca tggataactc
    12601 caatggtgtt gaggtggccc gacaaatgac tggaactcag cttggttccc ggcggggcga
    12661 agttccgctt tgcgcgcaat cgctagcccg ccaacaccaa gctggtgccg actaggagat
    12721 acggccgacg gcatcgatga tccaccgccg cagcgcgcca attcgacggt cgcgcgcctg
    12781 ttcagtacgg gacaccagat aaatggtctc agtgtgacgg gcctcgacat caaacggcgc
    12841 caccagcttc ttgccaaatc ccggtcgcgc cttgatcagc ggcgccatgg cgagggtgac
    12901 gccgaggcct tgctcggcgg cctccaacat cgccgggacg ctgtcgagcc aaagatgtcc
    12961 gcgcggggtc aattctgcaa tgccggcctc gttgagccag gccggccagg cgcgcggctg
    13021 cgaggtgagg tggatcagca cctgacggga taaatccttt ggcttgcgca ggccgctctt
    13081 gatcagcgcg ggcgcgcaga ccggcaaccc cgtgacctcg accagcggtt cggatcccaa
    13141 ttcgaacctg acggtccagc tgttcaggcc gccgtccggc gagtgggtgg gcgttcgcgc
    13201 cgagacccgg tggcgaccgg ctaccggatg tggggtcgga agcgggacgc tgctcgacac
    13261 gcgcggcgag gttggccgcg tctcgatgtc ggtcgtgctg gtgccatttc cgaaagcggc
    13321 agcggcggcg agctagcgca tcatccttcg agacggcgcg gagcctgtca tcgggccgcg
    13381 ctttgcgcgg acccgttggc gcctcctcag gatgaggtgt tagaccctcg tggtgaggag
    13441 cgcggcaacg ccgcgcgtct cgaatcatga ggccgtaacg gaccaacggt gctcacatgc
    13501 catggctggg gcttcgctag cctggttttg aggcttccaa cgccgtttag gttaacgcga
    13561 catcaataga cgttcgctgc aagaatcggt aagattggcg ctcgca
//
LOCUS       DATNOI010000488        19157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_153722,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000488 DATNOI010000000
VERSION     DATNOI010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 19157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19157
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153722"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VN968_19530"
     CDS             complement(<1..1089)
                     /locus_tag="VN968_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL31462.1"
                     /translation="MTQASFATHEVLNQSPPFEDVDLFALDAPLVEAVAANGGASAHA
                     ELSEFGQHWGSAAMAARGRIANENTPKLRTFDSRGNRRDEVEFHPAYHELMARSAHAG
                     VHNSTWNAEGKPAGGGAEVVRAAKFYMAAQVETGHLCPITMTRASVAALAAQPELLAQ
                     TMPVIGTRAYDPTFAPWPTKRGMTLGMGMTEKQGGTDVRSNQTRALRDGDAYRITGHK
                     WFMSAPMCDAFLVLAQADEGLTCFFMPRFAPDGSVNAIQFQRLKDKLGNRSNASSEVE
                     FTGAYAMRVGDEGKGIRTIIQMVQLTRQDCAIASAGLMRSGLAHALHHARHRSVFQKH
                     LADQPLMQAVLSDMALHVEASIALVMRLC"
     gene            complement(1162..1911)
                     /locus_tag="VN968_19535"
     CDS             complement(1162..1911)
                     /locus_tag="VN968_19535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HXL31463.1"
                     /translation="MKTIVIALVIVAYSAPVTAQSLPGGFVFLRDIDPTIMVDIRYAG
                     SNNFVGRPLRGYEAAECVVKRDVGLALKRVQQELAAQKLSLKMLDCYRPARAVADIAA
                     WAQNGSETTTDRRYNPSFSKTDLFRLGYIAERSGHSTGAAVDLTLVDLNAAKAPNFDP
                     TKAYADCTAPVGARAPEGSVDMGTGYDCSDAKAHTAAQSITPEQRRWRNLLVMAMARQ
                     GFVNYAKEWWHFSQPGAAGFAYDFPIISPRK"
     gene            2127..3080
                     /locus_tag="VN968_19540"
     CDS             2127..3080
                     /locus_tag="VN968_19540"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HXL31464.1"
                     /translation="MTPASKSTFVLGHRHLLGIEGLSAADITGLLDLSEEYVELNRQV
                     DKKRTSLRGRTQINLFFEASTRTQSSFEIAGKRLGADVMNMSVTSSSMRKGETLIDTA
                     VTLNAMHPDILVVRHHASGGVELLARKVDGSVINAGDGAHEHPTQALLDALTIRRNKG
                     RLEGLVIAICGDVMHSRVARSNILLLNTMGARVRVVAPSTLLPRGIERMGVEVARDMR
                     EGLNGADIVMMLRLQRERMNGSFVPSTQEYFHYFGLDQKKLAYAKPDALVMHPGPMNR
                     GVEIDSIVADGAQSLIREQVEMGVAVRMAVLEALARNLPNA"
     gene            3085..4386
                     /locus_tag="VN968_19545"
     CDS             3085..4386
                     /locus_tag="VN968_19545"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXL31465.1"
                     /translation="MLTDRRPILLANARLIDPSRDFDGPGDVLIADGVIRDARRGIGA
                     AGVPEGTDIVNCAGKVVAPGLIDMRAFVGEPGASHRETFASASQAAATGGITTIICQP
                     DTSPVIDNSATVDFVLRRARDTAIVNIHPMAALTKGMRGQEMTEIGLLKAAGAVAFTD
                     GDKSVTNAQVMRRTLAYARDFDALIVHHTEDPNLVGEGVMNEGEFAARLGLAGIPNAA
                     EAVMLERDMRLVALTGGRYHAASLSCIESLEILKRARDAGLSVSASVSINHVTLNEND
                     IGPYRTFLKLSPPLRTEQDRVALVAAVASGLIDVVMSDHNPQDVEVKRLPFAEAASGA
                     IGLETMLPASLRLIHNGEMDFKTLIRAMSTRPAELLGLPGGSLRAGSPADVIVIDADI
                     PWVLDPADLKSQCKNTPFDEARFLGRVVRTIIGGRTVYEHV"
     gene            4422..5018
                     /gene="plsY"
                     /locus_tag="VN968_19550"
     CDS             4422..5018
                     /gene="plsY"
                     /locus_tag="VN968_19550"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HXL31466.1"
                     /translation="MSPEAFLPVALILGYLLGSIPFGLVLTKLAGTADLRSIGSGNIG
                     ATNVLRTGRKGLAAATLICDVLKGTVAVVISGYYGGPNAAMLAALGAFLGHLFPVWLK
                     FRGGKGVAVYIGVLIGLFWPAAVVFCVLWAATAATSRYSSLSALVASFVTPLFLWWFG
                     HAALASLFAVLTMLLFYMHRENIKRLQAGTEGRIGENS"
     gene            complement(5057..5437)
                     /locus_tag="VN968_19555"
     CDS             complement(5057..5437)
                     /locus_tag="VN968_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL31467.1"
                     /translation="MATELKPIRTKADYKGALAEVERLSGAKSGTPKGDRLDVLATLI
                     DAYETRHYPMDPPDPIEAIQFRMEQQGLSRKDLEPMIGTRARVAEVMNRRRSLSIGMI
                     RRLHDQLGISAEVLIRPTREDEAA"
     gene            complement(5427..5771)
                     /locus_tag="VN968_19560"
     CDS             complement(5427..5771)
                     /locus_tag="VN968_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HXL31468.1"
                     /translation="MARTVTVRRDLTRSPLRMIVQSLSGRKDQPAVKAALDAWFDEAS
                     KASWKSTADVKRRHATASIVGAERIVFNIKGNDYRLVVAVDFEKGIVWIKWIDTHKAY
                     DKIDVTEVRHGN"
     gene            5873..6037
                     /locus_tag="VN968_19565"
     CDS             5873..6037
                     /locus_tag="VN968_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HXL31469.1"
                     /translation="MKRIFLKFQADESGATAIEYCLIAAFIALAIIAMVNALGIDVAG
                     ELAQINSSLK"
     gene            complement(6157..7398)
                     /locus_tag="VN968_19570"
     CDS             complement(6157..7398)
                     /locus_tag="VN968_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXL31470.1"
                     /translation="MMSLAELQRWIDSGDLSADAAITQSLEAIGADERTIGAFVCRAD
                     TVRAAAAGPLRGIAVGIKDIIDTADFPTQMGSAIYQGWRPRADAAVVAALKQAGASIA
                     GKTTTTAFASLDPTATLNPHNPQHTPGGSSSGSAAAVAAGMIPLALGTQTGGSVIRPA
                     SFCGVAAIKPSFKLLPTVGVKCYSWTLDTVGLFAASVKDLGCGLSAMTNRPELLPGAA
                     IGTPRIGVVTQDFAGTPEATGGEALRIATLAAERAGASVRALGLPEIIAEAWRIHSMV
                     QEFEAHQALAWEYRTHHDAMPPLLRAKLDESIGILPAVYDEARGISNRARRSLARVFE
                     EVDVLLTFSAPGAAPKGLDSTGDARFNRLWTLMGVPCVNIPAYVAEGGLPVGVQVIAR
                     FGDDAGALAAARFVEEALARR"
     gene            7588..8703
                     /gene="dprA"
                     /locus_tag="VN968_19575"
     CDS             7588..8703
                     /gene="dprA"
                     /locus_tag="VN968_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXL31471.1"
                     /translation="MDIRTPNTTHLTDEQRIDWLRLIRSDNVGPRTFRSLINHFGSAR
                     LALDRLPDLARRGGASRPGRICSEEDARTELAASKRHGVSLVAPGEAGYPPRLAVLDD
                     APPLLGVRGAFEALMRPMIAIVGSRNASGAGLKFAGQLARDLGEAGFVIASGLARGID
                     QAAHRASLTGGTVAMLAGGHDKVYPPEHEELLATIIETGGAAISEMPLGHVPRARDFP
                     RRNRLISGAALGVVVVEAALRSGSLITARIAAEQGREVFAVPGSPLDPRAAGTNDLIK
                     QGATLTTEASDVIDAVEPIMGRPIELREPDDDPLASEPDTSDRARIVNLLGPTPILLD
                     DLVRMAKTSPAIVRTVLLELDLAGRLERHGGGLVSLI"
     gene            complement(8707..9108)
                     /locus_tag="VN968_19580"
     CDS             complement(8707..9108)
                     /locus_tag="VN968_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL31472.1"
                     /translation="MIPEHTRVPVLPPNAHLDSDCRGVASILARVGDKWSVFVIMLLG
                     GGPRRFNEIKRMVGGISQRMLTLTLRGLERDGLVTRTVFPTIPPRVDYELTDLGRGLW
                     QPVEALGKWAADHQAEIEAARAKFDGRNDRG"
     gene            9205..9813
                     /locus_tag="VN968_19585"
     CDS             9205..9813
                     /locus_tag="VN968_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HXL31473.1"
                     /translation="MKLLHIDSSVLGPHSVSRQVSAAIVDRLRKAAPGLEIVYRDLTL
                     TPLAHLSGSHLSAGQGAAPEATLQQDLVAGQAVLNEFLAADIIVIGAPMYNFTLPSQL
                     KAWIDRIVVAGKTFKYGAQGAEGLAGNKRVIVAISRGGFYSAGTPAAAGEHLETYLRW
                     VFGFIGVTNPEFISADGIQVGPEHREKALANALQAATDLHAA"
     gene            9933..10205
                     /locus_tag="VN968_19590"
     CDS             9933..10205
                     /locus_tag="VN968_19590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31474.1"
                     /translation="MRTAIRDASKPMLENNAIVTSGLLSDLSNEPVDAEIGTKPRAFG
                     ISGFSNAYVVQTPQPEEQSSQLRRRFNALSEFREIPPPDDGTGQRN"
     gene            complement(10271..11044)
                     /locus_tag="VN968_19595"
     CDS             complement(10271..11044)
                     /locus_tag="VN968_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL31475.1"
                     /translation="MRLANKTALITGGNSGIGLATARLFVAEGARVAITGRNRTTLEA
                     AASELGPNALAIVADATDIAATEHAIRQAVEKFGKLDIVFANAGIPGNTPIGGTTLAA
                     FEQVIRTNITSVFFTVQAAAPHLNDGASIILNGSVISVLGNPGYSAYAASKAGVRAMA
                     RVMASELSPRGIRVNVVAPGAARTPIWNGAAPTAEAFAALDKRISRTIPLGRLGEADE
                     VAKTVLFLASDDASNVQGAEIFVDGGATGSPAGAPIYRG"
     gene            11165..11641
                     /locus_tag="VN968_19600"
     CDS             11165..11641
                     /locus_tag="VN968_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL31476.1"
                     /translation="MVKRTSLEKAECPIARSLDALGDWWSLLIIRDAFLGKRRFGEFQ
                     KGIGLAKNILTVRLRALVERGILTTAPASDGSAYQEYLLTQKGRGIFPVLVALRQWSE
                     EFSCDGGGFPTLLVDRDKGRPVRKLELRAEDGRLLGNGDTDVRPNSGAKRHRRVLA"
     gene            complement(11660..11803)
                     /locus_tag="VN968_19605"
     CDS             complement(11660..11803)
                     /locus_tag="VN968_19605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31477.1"
                     /translation="MAYIAENVADLARLARRHNLEILVRLLEMAQMEAQDRIRHRGKR
                     NLS"
     gene            12129..14942
                     /gene="topA"
                     /locus_tag="VN968_19610"
     CDS             12129..14942
                     /gene="topA"
                     /locus_tag="VN968_19610"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HXL31478.1"
                     /translation="MNIVIVESPAKAKTINKYLGSAYEVLASFGHVRDLPAKNGSVDP
                     EANFQMIWEVDPKAAGRLNDIAKALKGADRLILATDPDREGEAISWHVLEVLKEKRVI
                     KDHKIERVVFNAITKQAVTDAMKHPRQIDGALVDAYMARRALDYLVGFTLSPVLWRKL
                     PGARSAGRVQSVALRLVCDRELEIEKFIPREYWSLVATLTTPRGDAFEARLVGADGKK
                     IQRLDIGTGAEAEDFKKAIEAANFTVSTVEAKPARRNPQAPFTTSTLQQEASRKMGFA
                     PAHTMRIAQRLYEGIDIGGETTGLITYMRTDGVQIDNSAITQARKVIGEDYGNAYVPE
                     APRQYQTKAKNAQEAHEAIRPTDLSRRPAEMRRRLDTDQARLYELIWIRTIASQMESA
                     ELERTTVDIAAKAGSRVLELRATGQVVKFDGFLALYQEGRDDDSDDDDSRRLPAMSEG
                     EALKRRDLAVTQHFTEPPPRFSEASLVKRMEELGIGRPSTYASILQVLKDRGYVRLEK
                     KRLHGEDKGRVVVAFLENFFARYVEYDFTAALEEQLDRISNNEISWQQVLRDFWIDFI
                     GAVNEIKDLRVTQVLDALDDMLGPHIYAPREDGGDPRQCPTCGTGKLNLKAGKFGAFV
                     GCTNYPECRYTRPLAADSEASADRILGEDPVSGLDVVVKAGRFGPYIQLGEPKDYAEG
                     EKPKRAGIPKNMSPGDIELELALKLLSLPREIGKHPETGEPITAGLGRFGPFVRHEKT
                     YASLEAGDEVFDIGLNRAVTLIAEKILKGPSGRRFGADPGKPLGDHPTLGGVAVKGGR
                     YGAYVTAGGVNATIPSDKTQETITLEEAVALIDERVAKGGGKPKRGAKKAKPAKPAKA
                     EKPAKLEADTKAPKPANKPANKPANKPAKKAVAKKPATKPKSDSAAAASKARAPVTAT
                     AKTSPAKPAPKTAAKKSAGKASG"
     gene            14944..17280
                     /gene="rnr"
                     /locus_tag="VN968_19615"
     CDS             14944..17280
                     /gene="rnr"
                     /locus_tag="VN968_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HXL31479.1"
                     /translation="MAKQRDKVFPERDALVAFIRAHPGKVGTREIAREFGLKNADRVE
                     LKRILRELADEGTIAKRGKKISEPAALPATVMTDITGRDSDGELIATPTEWDEVENGE
                     PPKIRIHVPRRPQPGTAAGVGDRTLLRIEKLDEREGGAYRGRVIKVVDHAKTRVLGIF
                     RALPGGGGRLIPVDKKQAGRELNIAKADSNGAEDGDLVSVDLVRSRGFGLASGKVKER
                     LGSLASEKAVSLIAIHSHEIPQAFSPAALREAEEAKPATLKGREDWRDLPLVTIDPPD
                     AKDHDDAVHAQADRDPNNKGGYIVDVAIADVAFYVRPGSALDRDALTRGNSVYFPDRV
                     VPMLPERISNDLCSLVPGEPRGALAVRMIIGNDGRKRSHSFHRILMRSAAKLNYAQAQ
                     AAIDGRPDDVTGPLLDPILKPLYDAYAVVKRARDERDPLDLDIPERKILLKADGTVDR
                     VIVPERLDAHKLIEEFMILANVAAAEMLEKKALPLIYRVHDEPTLEKVHNLQEFLKTL
                     DLPFAKSGALRPALFNRVLAQVKGHDSEPLVNEVVLRSQAQAEYAAENYGHFGLNLRR
                     YAHFTSPIRRYADLVVHRALIRGLGLGEGALPETETVETLTEVAAQISVTERRAMKAE
                     RETADRLIAHFLADRIGATFQGRISGVTRAGLFVKLSDTGADGLIPIRTLGTEYFNYD
                     EARHALVGSRSGAMHRLGDVVDVRLVEAAPVAGALRFVLLSEGRLAPRGRAREAGRDV
                     SKARAKVLPGRSPRKQDHKSGKAKPGKSKPKKGKSWKP"
     gene            17268..17696
                     /locus_tag="VN968_19620"
     CDS             17268..17696
                     /locus_tag="VN968_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HXL31480.1"
                     /translation="METVSATSKVWSPESAPAEKRSVWTALRRGFRGRCPRCGEGKLF
                     RAFLKVDDRCSVCGLDFTPHRADDLPAYLVIVIVGHIVVPTILWIETDYAPPVPLQLA
                     IYLPLTLVLSLLLLQPVKGAVVGVQWALRMHGFDEQNPEP"
     gene            17741..18490
                     /locus_tag="VN968_19625"
     CDS             17741..18490
                     /locus_tag="VN968_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXL31481.1"
                     /translation="MSETVKEQKEADHHPYRRPVDAATLILVDRAAITPKVLVGKRHD
                     KVVFMPGKFVFPGGRVDKTDNRIPVAAPIPKALETNLMKGSPKITPSRARSLAIAAIR
                     EACEETGLCLGRKTDGAAPKLEGEWKPFSDAGLLPDPSGLFLIARAITPPGRVRRFDT
                     RFFTADASSIAHRVEGVIHAEAELVELVWVEIGSKPLADLHPMTRNVLNELEKRLATG
                     PLNHDAEVPFFHFYGGKMHKDVLAETRSAVS"
     gene            18487..>19157
                     /locus_tag="VN968_19630"
     CDS             18487..>19157
                     /locus_tag="VN968_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXL31482.1"
                     /translation="MIRFTKFLLASVALCSLLSLPARAELDVAKLKTTIEASVENEYP
                     KLDALYKDIHAHPEIAFQEVKTAAKLAAEMRAIGFEVTEQVGKTGLVAIYKNGDGPTI
                     MVRTELDALPMEEKTGLPYASHDKTNWHGRETPVAHSCGHDIHMASWVGTAKTLVGLK
                     DQWHGTLMFIAQPAEEEIAGAKAMLDDGLFTRFPKPDVGFALHDGASAYGTVSYRVGI
                     GSSNQ"
BASE COUNT         3608 a         6148 c         5968 g         3433 t
ORIGIN      
        1 gcacagccgc atgacaagcg caatcgaagc ctcgacatgc agcgccatgt ccgacagcac
       61 cgcctgcatc agcggctgat cggcgagatg tttttggaac acgctgcgat ggcgggcgtg
      121 gtgcaatgca tgcgcgagcc cggatcgcat caggccggcg gacgcaatcg cgcagtcctg
      181 ccgcgtcagc tgcaccatct ggatgatggt gcggatgccc ttgccctcgt cgccgacccg
      241 catcgcatag gcgccggtga attcgacttc ggaggacgca ttggagcgat tgccgagctt
      301 gtccttcagg cgctggaact ggatcgcgtt gaccgatccg tccggcgcaa aacgcggcat
      361 gaagaagcat gtgagccctt cgtcggcctg cgccagcacc aggaaggcgt cgcacatcgg
      421 cgccgacatg aaccatttat gcccggtgat gcgataggcg tcgccgtcgc gcaacgctcg
      481 cgtctggttg gaacgcacgt cggtgccgcc ctgcttctcg gtcatgccca tgccaagcgt
      541 catgccgcgt tttgtcggcc acggcgcaaa ggtcggatcg taagcgcgtg tgcctatgac
      601 cggcatcgtc tgggcgagca gttccggctg cgccgccagt gccgccaccg acgcccgcgt
      661 catggtgatc ggacagagat gaccggtctc gacctgggcc gccatataga actttgccgc
      721 acgcacgact tcggccccac cgccggccgg cttgccctcc gcgttccagg tcgaattgtg
      781 cacgccggcg tgcgcactgc gcgccatcaa ctcgtgatag gcgggatgaa actcgacttc
      841 gtcgcggcga ttgcctcggg aatcgaacgt ccgcagcttt ggcgtgttct cgttggcgat
      901 gcggccccgc gccgccatgg ctgccgaacc ccagtgctgc ccgaactcgg acaattccgc
      961 gtgcgccgaa gcgccgccat tggccgcgac cgcctccacc agcggggcgt ccagcgcgaa
     1021 caggtcgacg tcttcgaacg gcggcgactg gttgagcacc tcgtgggtcg cgaatgaagc
     1081 ctgggtcatg gctgttcctg actgtcggag atttcgggaa tcagtttacg ccggaatggc
     1141 cccaatttca cgcggccttg ttcacttgcg aggcgagatg atcggaaaat catatgcgaa
     1201 gccggctgcg cccggctgcg aaaaatgcca ccattccttg gcgtagttca caaagccttg
     1261 ccgagccatg gccataacca gcagattccg ccaacggcgt tgttccggcg tgatggattg
     1321 cgcggccgta tgcgcctttg cgtcggaaca atcatagccg gtgcccatgt cgacgctacc
     1381 ttccggggcg cgggcgccga caggcgcggt gcagtcggcg taggccttgg tgggatcgaa
     1441 attgggtgcc ttagcggcgt tcaaatcgac caaggtgagg tcgaccgcgg caccggtgga
     1501 atggccggaa cgctcagcga tatagcccag ccggaacaga tccgtcttgc tgaatgacgg
     1561 attgtagcga cgatcggttg ttgtctcgct gccgttctgg gcccatgccg cgatatcggc
     1621 gaccgcgcgc gccggccggt agcaatccag cattttcagc gacagttttt gggcggctag
     1681 ttcctgctgg acgcgcttca gcgccagccc gacgtcgcgc ttcaccacgc attcggcggc
     1741 ctcgtagccc ctgagcggcc ggccgacgaa attgttcgat ccggcgtagc ggatatccac
     1801 catgatcgtc ggatcgatat cgcgcaagaa cacgaaaccg ccgggcaagg actgcgcggt
     1861 taccggagcg gagtacgcga caatcacaag ggcaatcaca attgttttca cgaaagccgt
     1921 tcccacacca tgtggccgga ctggttccgg ccatccacgt cttatttagg gttaattaca
     1981 aagcaaggcg tggaggcccg cgacatttgc cgcgcaggcg cgctttcgcg cggacatgac
     2041 gaggatggga cagaccgggg gataactcgc aacgccccta ttcgcgcttg ctctagcttg
     2101 catctctgcc tatagctctc gttttaatga cccctgcatc gaaatcgacc ttcgtcctcg
     2161 gtcaccggca cctgctggga atcgaggggc tttccgctgc cgatattacc ggcctgctcg
     2221 acctttccga ggaatatgtc gagctcaacc gccaggtcga caagaagcgg acctccctgc
     2281 gcggccgcac ccagatcaac ctgttcttcg aggcctcgac ccgcacccaa tcctcgttcg
     2341 aaatcgcggg aaagcgcctc ggcgccgacg tcatgaacat gtcggtgact tcctcctcga
     2401 tgcgcaaggg ggaaaccctg atcgacaccg cggtgacgct gaacgccatg cacccggaca
     2461 tcctggtggt gcggcaccat gcctcgggcg gcgtcgagct attggcgcgc aaggtcgacg
     2521 gttccgtgat caacgccggc gacggcgccc acgaacatcc aacgcaagcg ctgctcgatg
     2581 cgctgaccat ccgccgcaac aagggccgcc tcgaagggct cgtcattgcg atctgcggcg
     2641 acgtcatgca ttcgcgggtg gcgcgctcca acatcctgtt gctcaacacc atgggcgcgc
     2701 gggtgcgcgt ggtggcgcct tccacgctgc tgccgcgcgg catcgagcgg atgggcgtcg
     2761 aggtggcccg cgacatgcgc gagggcctca atggcgccga cattgtcatg atgctgcggc
     2821 tgcagcgcga gcgcatgaac ggctcgttcg tgccgtccac ccaggaatat tttcattact
     2881 ttggcctcga ccagaagaag ctggcgtatg ctaaacccga tgcgctggtg atgcatccgg
     2941 ggccgatgaa ccggggcgtc gagatcgact cgatcgtggc ggatggcgcg caatcgctga
     3001 tccgcgagca ggtggaaatg ggagtggcgg tgcggatggc ggtgctcgaa gcgctcgccc
     3061 gcaacctgcc gaacgcgtga catcatgctg accgaccgcc gcccgatcct gctcgccaac
     3121 gcccgcctga tcgatccgtc gcgcgatttc gacggccccg gcgatgtcct gatcgccgac
     3181 ggcgtgatcc gcgacgccag gcgcggcatc ggtgcggccg gcgtgcccga gggcaccgac
     3241 atcgtcaatt gcgccggcaa ggtggtcgcg cccggactga tcgacatgcg cgcctttgtc
     3301 ggcgagcccg gcgccagcca ccgcgaaacc tttgcttccg caagccaggc cgcggcgacc
     3361 ggcggcatta ccaccatcat ctgccagccc gatacctcgc ccgtcatcga caattcggcg
     3421 accgtggatt ttgtcctgcg ccgtgcccgc gacaccgcga tcgtcaacat ccaccccatg
     3481 gcggcactca caaaaggcat gcgcggccag gagatgaccg agatcgggct gttgaaagcc
     3541 gcgggcgcgg tggccttcac cgacggcgac aaaagcgtca ccaatgcgca ggtgatgcgg
     3601 cgaacgctgg cttatgcccg ggatttcgac gcgctgatcg tgcatcacac cgaagatccg
     3661 aatctggtcg gcgaaggcgt catgaatgaa ggcgagttcg ccgcacggct cgggctggcc
     3721 ggcatcccca atgccgccga ggccgtgatg ctggagcgcg acatgcgcct cgtcgcgctg
     3781 accggcggac gctatcacgc ggcgtcgcta agctgcatcg agtcgctgga aatcctcaaa
     3841 cgcgcgcgcg atgccggtct atcggtttcc gcctcggtct cgatcaacca tgtgacgctg
     3901 aacgaaaacg acatcggccc ctaccggacc ttcctaaagc tgtcgccgcc gctgcgcacc
     3961 gagcaggatc gtgtggcgct ggttgccgcc gtcgcctccg gcctgatcga cgtcgtcatg
     4021 tccgatcaca atccgcagga cgttgaagtg aagcggctgc cgttcgcgga agcagcctca
     4081 ggcgcgatcg gtctcgaaac catgctgccg gccagcttaa ggctgatcca caatggcgag
     4141 atggacttca agacgctgat ccgcgcgatg tcgacgcgcc ccgccgagct cttggggctg
     4201 ccgggcggat cgctgcgcgc gggctcgcct gccgacgtca tcgtgatcga tgccgacata
     4261 ccgtgggtgc ttgaccccgc cgatctcaag tcgcaatgca agaacacgcc gttcgacgaa
     4321 gcgcggttcc taggccgcgt ggtgcgcacc atcatcggcg gacgaacggt ttatgagcat
     4381 gtctgatgcg cgggaaatat ttcgcaaccg gagcagcggc gatgtcacct gaagcattct
     4441 tgcccgtggc gttgatcctc ggctatctcc tgggctccat accgttcggg ctggtgctga
     4501 caaagcttgc cggcacggcg gatctgcgct cgatcggctc cggcaatatc ggcgccacca
     4561 acgtgctgcg caccggccgc aagggcctcg cagccgcgac gctgatctgc gacgtgctga
     4621 agggcaccgt cgcggtggtg atatccggct attacggcgg ccccaatgcc gcgatgctgg
     4681 cggcgctcgg cgcctttctc ggccatttgt ttccggtctg gctgaaattc aggggcggca
     4741 agggcgtcgc ggtctatatc ggcgtgctga tcggactgtt ctggcccgcc gcggtggtgt
     4801 tttgcgtgtt gtgggccgcg actgctgcga cctcgcgcta ctcgtcgctc tcggcgctgg
     4861 tcgcgagctt tgtcacgccg cttttcctgt ggtggttcgg ccatgccgcg ctggcctcat
     4921 tgttcgccgt gctgaccatg cttttgttct acatgcaccg cgaaaacatc aagcggctgc
     4981 aggctggcac cgagggacgg atcggggaga acagttagcc ggggccgccg gttggttgat
     5041 ccgggctacg tgccgatcac gccgcctcgt cctcgcgtgt cgggcggatc agcacctcgg
     5101 cggaaatccc aagctggtcg tgcagacgcc ggatcatgcc gatggacagg ctgcgcctgc
     5161 ggttcatgac ctctgcgacg cgcgcgcgtg ttccaatcat cggttcgagg tccttgcggc
     5221 ttaggccttg ctgttccatc cgaaactgga tcgcctcgat cggatcgggc ggatccattg
     5281 gatagtgccg tgtttcataa gcgtcgatca gtgtcgccaa cacatcgagc cgatcgccct
     5341 tcggagtccc gctctttgcg cccgacaggc gctcaacttc ggcgagcgca cccttgtagt
     5401 ccgcctttgt gcggatcggt ttcagttcag ttgccatgtc gcacctcggt cacatcgatc
     5461 ttgtcatagg ctttgtgggt gtcgatccac ttgatccaga cgatgccctt ctcgaaatcg
     5521 accgctacca cgaggcgata atcgttaccc ttgatgttga agacgattcg ctccgcaccg
     5581 acgatactcg ccgtcgcatg acgccgtttg acatctgccg tactcttcca agaagccttg
     5641 ctcgcttcgt cgaaccaggc atccagtgcg gctttcactg ccggctgatc cttcctaccc
     5701 gaaaggcttt ggacgatcat gcgaagcggc gaccttgtta aatcccttct gaccgtcacc
     5761 gtccgcgcca tcgcttgtca tgaattgacc aaccgaaatt tacgcaacct taggttccgc
     5821 aagggcatga atcgcatcgg gaacatcccg cgcaacctga aaggatgcga aattgaagcg
     5881 aatttttctc aaattccagg ctgatgagtc cggcgccact gccattgaat attgtctgat
     5941 cgcagcgttc atcgcgctcg ccattatcgc gatggtcaat gccctcggta tcgacgtggc
     6001 cggcgaactc gcccaaatca acagctcctt gaaatagaaa accccaggtc gttctgctca
     6061 gcctttctgt tcagtcttta tctgtccagg tcttcttctg tgcacgtctc tccaagagcc
     6121 gccgccggct ttgaccagcg gcggtttgca gcctactcat cgccgcgcca gcgcctcctc
     6181 cacgaagcgt gcggcggcca gcgcgccggc gtcgtcgcca aatcgggcga tgacctgcac
     6241 gcccaccggc aaaccacctt cggcgacgta tgcgggaatg ttgacgcagg ggacgcccat
     6301 cagtgtccag agccggttga agcgggcatc gccggtcgag tccagtccct tgggcgcagc
     6361 gcctggggcc gagaacgtca gcaacacatc gacctcctca aaaaccctcg cgagggaacg
     6421 tcgggcccgg ttcgaaatcc cgcgcgcctc gtcgtagacg gcaggcaaaa tgccaatgct
     6481 ttcgtcgagt tttgcgcgca acagcggcgg catggcatcg tgatgcgtgc gatattccca
     6541 tgccagcgcc tgatgcgcct cgaattcctg caccatcgag tggatccgcc aggcttccgc
     6601 tatgatctcc ggcaggccaa gcgcgcgaac cgaggcaccg gcgcgttccg ccgcgagcgt
     6661 cgcgatccgc agcgcctcgc cgcccgttgc ttccggcgtg cctgcaaaat cctgcgtcac
     6721 gacaccgatg cgcggcgtcc cgatcgcggc gcccggcagc agttcgggcc ggttggtcat
     6781 cgccgaaagc ccgcatccga gatctttgac gctggccgcg aatagcccga ccgtgtccag
     6841 cgtccacgaa taacatttca caccgaccgt cggcaaaagc ttgaacgacg gtttgatggc
     6901 ggctacgccg cagaacgatg ccggccggat caccgaaccg ccggtctgcg ttcccagcgc
     6961 aagcggaatc attccggccg cgacggccgc cgccgagccc gatgacgatc cgcccggcgt
     7021 atgctgtgga ttgtgcggat tgagggtcgc ggtcggatcg agggaggcga acgccgtggt
     7081 cgtggtcttg ccggcgatac tcgcgcccgc ctgcttcagg gcggccacca cagccgcatc
     7141 cgcgcgcggc cgccagcctt ggtagatcgc cgagcccatt tgtgtcggaa aatccgccgt
     7201 gtcgatgatg tccttgatcc cgacggcgat gccgcgcaac ggcccggcag ctgcggcgcg
     7261 cacggtgtcg gcacggcaga cgaaggcgcc gatggtcctt tcgtccgcgc cgatcgcctc
     7321 aagcgactgg gtgatggcgg catcggcgga aagatcgccg gaatcgatcc agcgctggag
     7381 ttcggcgagc gacatcatcg gcattgatcc ccttaggcga ccggaccgtt tttacggagc
     7441 gcgtggggat tgggcaagcg tccctcccgt cattccgggc ccgcgccaag tggcgcaggc
     7501 ccggacgaga gttgacggga tcccgccccg ttcaaaacga gttgatcgcc ccttccggtt
     7561 gggcgaagct gcgcaggcag gggccgcatg gacatcagaa cacccaacac cactcacctc
     7621 accgacgagc agcggatcga ctggctgcgg ctgatccgtt cggacaatgt cgggccgcgc
     7681 acctttcgtt cgctgatcaa tcatttcggc agcgcccgct tggcgctgga ccgcctgccc
     7741 gatctggcgc gccgcggcgg cgcatcgcgg ccgggccgca tctgcagcga ggaagacgca
     7801 cgcacggagc ttgccgcgag caaacgacac ggcgtcagcc tggtggcgcc gggcgaagcc
     7861 ggatatccgc cgcggctggc cgtgctcgac gatgcgcctc ccctgctcgg cgtgcgcggc
     7921 gcgtttgaag cgctgatgcg gccgatgatt gcgatcgtcg gctcgcgcaa cgcgtcagga
     7981 gcgggcctca aatttgcggg ccagctggcg cgcgatcttg gcgaggccgg ttttgtcatt
     8041 gcctcggggc tggcgcgcgg catcgatcag gcggcacatc gcgccagcct cacgggcggc
     8101 accgtggcga tgctggccgg cggtcatgac aaggtctatc cgcccgagca cgaagaactg
     8161 ctggcaacga tcattgagac cggcggggcc gcgatttccg aaatgccgct cggtcacgtg
     8221 ccgcgcgccc gcgattttcc gcggcgcaac cggctgatct cgggtgcggc gctcggcgtc
     8281 gtcgtggtcg aggccgcgct tcgttcgggt tcgctgatca cggcgcggat cgccgccgag
     8341 cagggccgcg aggtgttcgc ggtgccgggc tcgccgctcg atccgcgcgc cgccggcacc
     8401 aacgacctga tcaaacaggg cgcgacgctg accacggaag cgtcagacgt catcgatgcg
     8461 gtcgagccga tcatggggcg gccgatcgag ctgcgtgagc cggatgatga tcctctggca
     8521 tccgaacccg acacgagcga tcgcgcccgc attgtcaatc tgttgggacc aacgccgatc
     8581 ctgctcgatg atctcgtccg gatggccaaa acctcgccgg cgatcgtgcg caccgtgctg
     8641 ctcgagcttg acctcgcagg acggctcgag cgtcacggcg gcgggctggt gtcgctgatc
     8701 taacgatcac ccgcgatcgt tgcgcccgtc gaacttcgcg cgcgcggctt cgatctccgc
     8761 ctgatgatcc gccgcccatt tgcccagcgc ttcgaccggc tgccacaggc cgcgtccgag
     8821 atcggtcagt tcgtaatcca cgcgaggcgg aatggtcggg aacaccgtgc gcgtcaccag
     8881 gccgtcgcgc tcgagcccgc gcagcgtcag ggtcagcatc cgctgcgaga tgccgccgac
     8941 catgcgcttg atctcgttga agcggcgcgg gccgcctccg agcagcatga tgacgaacac
     9001 gctccattta tcgccgaccc gggccaggat cgaagcgacg ccgcggcaat cgctatcgag
     9061 gtgcgcgttg ggcggcagga caggcactct ggtgtgctcg ggtatcatgg atgtgctcgg
     9121 gtttcaaaaa tgtgcgttct tgcggagttt gcggacagtc actcatatag tctcagttac
     9181 aaacttatac caagggtgac catcatgaaa cttcttcaca tcgattccag tgtcctcggc
     9241 ccccactccg tcagccgcca agtttccgcc gcgattgtgg atcgtctgcg caaggcggcg
     9301 ccgggtctcg aaatcgtcta tcgcgaccta accttgacgc cgctggcgca cctgtcgggc
     9361 tcgcatcttt ctgccgggca aggggcggca cctgaggcca cgctccagca ggacctcgtc
     9421 gccggtcagg ccgtgctgaa cgagtttctg gccgccgaca tcatcgtgat cggcgcgccc
     9481 atgtacaatt tcacgcttcc aagccagctc aaggcttgga tcgaccgtat cgtggttgcc
     9541 ggcaagacct ttaaatatgg cgcgcagggc gcggagggat tggccggcaa caagcgcgtc
     9601 attgtcgcga tctcgcgcgg cggcttttac agcgccggca cgccggcggc ggccggcgaa
     9661 catctggaaa cctatttgcg ctgggtgttc ggctttatcg gcgtcacgaa cccggaattt
     9721 atttccgccg acggaatcca ggtcggaccc gaacaccgcg agaaagcgct ggccaatgcc
     9781 ctgcaagcgg ccaccgatct ccacgcggca tagcaagagc gagctgagac accgcctgcc
     9841 gccatcagcg gtgacggcgg gctggtgtcg ttcctataaa ctcattcgtg tgaaatgaag
     9901 gttgtgatta gtaccatacg ccttgcacgc ccatgagaac ggcgatcagg gatgccagca
     9961 agccgatgct ggaaaacaac gctatcgtaa caagcgggct attgtcggat ctgtcgaatg
    10021 aaccggttga cgcggaaatc ggcacaaaac cacgggcttt cggcatttca ggcttctcca
    10081 atgcatacgt ggttcaaact ccccagcctg aggaacagtc gtcgcagctg cgacgaaggt
    10141 tcaacgcact cagtgaattc cgcgaaatac ctccaccaga tgacggaacc ggacaacgca
    10201 actgacagga ctattcgtca gatcacgctg cccggatgca atgattttgg attttggctg
    10261 gagcttgcgt ctagccgcga tagatcggcg cgcccgcggg cgaacccgtc gcgccgccat
    10321 cgacgaagat ctccgcgcct tgaacgttgg aagcatcgtc ggaggcaagg aacaacaccg
    10381 tcttggcaac ttcgtcggct tcgccgagcc gcccgagcgg aatggtgcgg gaaattcgct
    10441 tgtccagagc agcgaatgct tcggccgtgg gagccgcacc attccagatc ggcgtccttg
    10501 ccgcgccggg cgcaaccaca ttgacgcgaa tgccgcgcgg cgagagttcc gatgccatca
    10561 cccgggccat cgcccgcacc ccggccttgc tcgccgcata ggccgagtag ccgggattgc
    10621 cgagcacgga gatgaccgag ccgttcagga tgatcgacgc gccgtcgttc agatgcggtg
    10681 ccgccgcctg cacggtgaag aacaccgagg tgatgttggt tctgatgacc tgctcgaatg
    10741 cagccagcgt ggtccctccg atcggcgtat tgccggggat gccggcattg gcgaagacga
    10801 tgtcgagctt gccgaatttt tccacggcct gccgaatcgc atgttcggtc gcggcgatat
    10861 cggtcgcgtc ggcgacaatc gcaagcgcat tcggtcccag ttcgcttgcg gcggcttcca
    10921 gcgtcgtccg atttcgtccg gtgatggcga cccgcgcgcc ttcggccacg aacagccgcg
    10981 ccgtcgcaag tccgatgccg ctgttgccgc cggtaatcaa cgccgtcttg ttcgcaagcc
    11041 tcatgatcgc ctccagtggt tgcattatta aactagatgg actacctagg cggtctggtt
    11101 tcataatgca actacacaac gccgtgatcg gcgggattca tgttttggag ccgaaaggac
    11161 gcccatggtg aaacgcacca gtcttgaaaa ggcggaatgc ccgatcgcgc ggtcgctgga
    11221 cgcgctcggc gactggtggt cgctgctgat catccgcgat gccttcctgg gaaagcgccg
    11281 cttcggcgaa tttcaaaaag gcatcggcct ggcgaagaat atcctcaccg tgcggctgcg
    11341 cgcgttggtg gagcgcggca tcctgacgac ggcgcccgcc tccgacggca gcgcctatca
    11401 ggaatatctg ctgacccaga aggggcgcgg gatatttccg gtgctggtcg cgctgcggca
    11461 gtggagcgag gaattttcct gcgatggcgg tggcttcccc accctgctgg tcgatcgcga
    11521 caaaggccga ccggtgcgaa aactcgaact ccgcgcggag gatggccggt tgctcggcaa
    11581 tggcgatacc gacgtacggc cgaattctgg tgccaaacgc caccggcgcg ttctggcctg
    11641 agcgatggca gcctgacatt taagacaaat tgcgtttgcc gcgatgccga atgcgatcct
    11701 gcgcttccat ctgcgccatc tcgagcaggc gaacgagtat ttcgaggtta tggcgccgcg
    11761 ccaaccgggc gaggtcggcg acgttctccg cgatgtaggc caaagcctca tccggcccgc
    11821 cgtcccccgg cggatcatcg gttcgcgaac tcttatccca gcccgaagat gcggccctcc
    11881 gccgacgtcc tgtcttggtc aattaaaaac acatccaatg ccgtgcattg gtgacagata
    11941 ccctttttga gagcgcaact ctaggacgca tttttcaact ttttggatac tggttccccc
    12001 tgttcccgca ccctttcaac atctgtcgat ttgacagggg cccccactac acccatgttc
    12061 cctgcgaaac ggccgacgcg aatctcgcgg aaccggccct tattttcccc ttaagccatt
    12121 ggaataacat gaatatcgtc attgtcgagt cgccggcgaa agccaagaca atcaataaat
    12181 atttgggctc cgcctatgag gtcctggcct cattcggcca tgttcgggat ctgccggcca
    12241 agaatggatc ggtggatccg gaggccaatt tccagatgat ctgggaggtc gaccccaagg
    12301 cggcgggccg actcaacgac atcgccaagg cgctcaaggg tgccgaccgg ctgatcctcg
    12361 caaccgaccc cgaccgcgag ggcgaagcga tctcatggca cgtgctggag gtcttgaagg
    12421 aaaagcgcgt gatcaaggac cacaagatcg agcgcgtggt gttcaacgcc atcaccaagc
    12481 aggccgtgac cgacgcgatg aagcatccgc gccagatcga tggcgcgctg gtcgatgcct
    12541 acatggcgcg ccgtgcgctg gactatctgg tcggcttcac gctctcaccg gtgttgtggc
    12601 gcaaattgcc gggcgcgcgc tccgcgggga gagtgcagtc ggtcgcgctc cggctggtct
    12661 gcgaccgcga gctcgagatc gagaaattca ttccgcggga atattggtcg ctggtggcga
    12721 ccttgacgac gccgcgcggc gacgcgttcg aggcccggct ggtcggagcc gacggcaaga
    12781 agatccagcg gctcgacatc ggcaccggcg cggaagccga agatttcaaa aaggcgatcg
    12841 aggccgccaa ttttaccgtc tccaccgtgg aggcaaaacc ggcgcgtcgc aatccgcagg
    12901 cgccgtttac cacatcaacg ctgcagcagg aggccagccg caagatgggc tttgcgcccg
    12961 cgcataccat gcggatcgcg caacggcttt atgaaggtat cgatatcggc ggcgagacca
    13021 ccggtctcat tacctatatg cggaccgacg gcgtgcagat cgacaattcg gcgatcaccc
    13081 aggcgcgcaa ggtgatcggc gaggattatg gcaacgccta cgtgccggag gcgccgcgcc
    13141 agtaccagac caaggccaag aacgcccagg aggcgcatga agcgatccgt ccgaccgatc
    13201 tgtcgcgccg tccggccgag atgcgccgcc ggctcgatac cgatcaggcg cggctctatg
    13261 aattgatctg gatccgcacc atcgccagcc agatggaatc ggccgaactt gaacgcacca
    13321 cggttgatat cgcggcgaaa gccggctccc gcgtgctgga attgcgcgcg acagggcaag
    13381 tggtgaaatt cgacggcttc ctcgcgctct atcaggaagg ccgcgacgac gatagcgacg
    13441 acgacgactc tcgccgcctc cccgccatga gcgagggcga ggcgctgaag cgcagggatc
    13501 tcgccgtcac ccagcatttt accgaaccgc cgccgcgttt ttccgaagct tccctggtca
    13561 agcgcatgga ggagctcggc atcggccgtc cctcgaccta tgcctcgatc ctgcaggtgc
    13621 tgaaagatcg cggctatgtc aggctcgaaa agaagcgcct gcatggcgag gacaagggcc
    13681 gcgtcgtggt cgcgttcctg gaaaactttt tcgcccgcta cgtcgaatat gattttaccg
    13741 ccgcgctgga ggagcagctc gaccgcatct ccaacaacga gatttcctgg cagcaggttt
    13801 tgcgggattt ctggatcgat ttcatcggcg ccgtcaacga gatcaaggat ctgcgtgtca
    13861 cccaggtgct ggacgcgctc gacgacatgc tggggccgca catctacgcg ccgcgcgagg
    13921 atggcggcga tccccggcaa tgcccgacct gcggcaccgg caagctcaac ctcaaggccg
    13981 ggaaattcgg cgcctttgtc ggctgcacca actatccgga gtgccgctat acgcgcccgc
    14041 tggccgccga cagcgaagcc agcgccgatc gcatcctcgg cgaggatccg gtgtccggtc
    14101 tcgacgtcgt ggtgaaggcc ggtcgcttcg gtccctacat ccagctcggc gagcccaagg
    14161 actacgcgga aggcgaaaag ccgaagcgtg ccggcattcc caaaaacatg tcgcccggcg
    14221 acatcgaact cgagctggca ttgaagttgt tgtcgctgcc gcgcgaaatt ggaaaacatc
    14281 cggaaaccgg cgaaccgatc accgcggggc tcggtcggtt cggcccgttc gtgcgccatg
    14341 agaaaaccta tgccagcctc gaggccggtg acgaggtgtt cgatatcggg ctcaaccggg
    14401 cggtcacgct gattgccgag aaaatcctga agggaccgag cggccgccgc ttcggcgccg
    14461 atcccggcaa gccgctgggc gatcatccga cgcttggcgg cgtcgcggtc aagggcggac
    14521 gttacggcgc ctatgtcacg gcgggcggcg tcaacgccac gatccccagc gacaagacac
    14581 aggaaaccat cacgctcgaa gaagccgtcg cgttgatcga cgaacgcgtc gccaagggcg
    14641 gcggcaagcc gaagcgcggc gccaagaaag caaaacccgc caagcctgcc aaagcagaga
    14701 agccggccaa actggaagcg gacaccaagg cgcccaaacc tgccaacaag cctgctaata
    14761 agcccgccaa caagcctgcc aagaaggccg tcgcgaagaa acccgcgacc aagccgaaat
    14821 cggatagcgc cgccgcggcc agcaaggccc gcgcgccggt aacagcaacg gccaaaacct
    14881 ctccggcgaa gccagcaccc aaaaccgccg ccaagaagag cgccggcaag gcaagcggat
    14941 aaagtggcaa aacagcgcga caaggttttt ccagagcggg acgccctcgt cgcctttatc
    15001 cgcgcgcatc cgggaaaagt cggcacccgc gaaatcgcgc gggaattcgg cctgaagaac
    15061 gccgaccgcg tcgaactcaa gcgcatcctg cgggaactcg ccgacgaagg cacgattgca
    15121 aaacgcggca agaagatcag cgaacctgca gccctgccgg cgaccgtgat gaccgacatc
    15181 accggccgtg acagcgacgg cgaattgatc gccacgccca ccgaatggga tgaggtggaa
    15241 aacggcgagc cgccgaaaat ccgcatccat gtaccgcgcc gtccgcagcc aggcacggcg
    15301 gcaggtgtag gcgaccgcac gctgctgcgg atcgagaaac tcgacgagcg cgaaggcggc
    15361 gcctatcgcg gccgcgtcat caaggtggtc gatcacgcca agacccgcgt gctcggaatt
    15421 tttcgcgcgc tccccggcgg cggcgggcgg ctcattccgg tcgacaagaa gcaggccggc
    15481 cgtgaattga acattgccaa ggccgacagc aatggcgccg aggacggcga cctcgtcagc
    15541 gtcgacctgg tgcgctcgcg cggtttcggt ctggcctccg gcaaggtgaa ggaacggctt
    15601 ggctcgctcg ccagcgagaa ggccgtcagc ctgatcgcca ttcattccca cgaaattcca
    15661 caagccttct cgccggccgc gttgcgcgag gccgaggagg caaagcccgc gacgctcaaa
    15721 ggccgcgaag actggcgcga cctgccgctg gtgacaatcg atccgcctga cgccaaggat
    15781 catgatgacg cggttcatgc gcaagccgat cgcgatccga acaacaaagg cggctacatc
    15841 gtcgatgtcg cgatcgccga tgttgcgttc tatgtgcggc cgggatcggc gctcgatcgc
    15901 gacgcgctga cgcgcggcaa ttcggtgtac tttccggatc gtgtggtgcc gatgctgccc
    15961 gagcgcatct ccaacgatct gtgctcgctg gtgccgggcg agccgcgcgg cgcgctggcg
    16021 gtgcggatga taatcggcaa tgacggccgc aagcgctcgc acagctttca tcgtatcctg
    16081 atgcgatcgg cggcgaaact gaactacgcg caggcgcagg cggcgatcga cggacggccc
    16141 gacgatgtaa ccggccccct gctcgatcca atcctaaaac cgctctacga cgcctatgcg
    16201 gtcgtgaaac gcgcgcgcga cgaacgcgat ccgctcgacc tcgatattcc cgagcgcaag
    16261 atcctcttga aagcggacgg cacggtcgat cgcgtgatcg tgccggagcg gctggacgcg
    16321 cataaactga tcgaagaatt catgatcctg gccaatgtcg ccgcggccga gatgctggaa
    16381 aagaaggcgc ttccgcttat ctatcgggtg cacgatgaac cgacgcttga gaaggttcat
    16441 aacctgcagg aattcttgaa aacgctcgat ctgccgttcg ccaaaagcgg cgccttgcgg
    16501 cccgctttgt tcaatcgcgt gctggcgcag gtcaagggac atgattccga gccgctggtg
    16561 aatgaagtgg tgttgcgctc gcaagcccag gccgaatacg ccgccgagaa ttacggccac
    16621 ttcggcctga acctgcggcg ctatgcgcat ttcacttcgc cgatccgccg ctacgccgat
    16681 cttgtcgtgc accgcgcctt gatccggggc ctcggtcttg gcgaaggcgc gctgccggag
    16741 actgagacgg tggaaacgct gaccgaggtc gcggcgcaga tctccgtgac cgagcgccgc
    16801 gccatgaagg ccgagcgcga aaccgccgac cggctgatcg cgcattttct cgccgaccgc
    16861 atcggggcca cctttcaggg ccgtatctcc ggcgtcaccc gcgccggact gttcgtgaaa
    16921 ctctccgata ccggcgccga tgggctgatc ccgatccgca ccttgggcac ggaatatttc
    16981 aactacgatg aggcgcgcca tgcgctggtc ggatcgcgca gcggtgccat gcaccggctg
    17041 ggtgacgtcg tcgacgtccg tctggtagaa gcagctccgg tggcaggtgc gttgcggttt
    17101 gtactgctgt cggaaggccg ccttgccccg cgcggcagag cgcgcgaagc cgggcgtgat
    17161 gtttccaagg cgcgggccaa agtgcttcct ggccgtagcc cgcgcaagca agatcacaag
    17221 tcaggcaagg ccaagcccgg caaatcgaag ccgaagaagg gcaaatcatg gaaaccgtga
    17281 gcgcaacaag caaagtctgg agcccggaat ccgcgccggc cgaaaagcgc agcgtctgga
    17341 ccgcattgag gcgcggtttt cgcgggcgct gcccccgctg cggcgaaggc aaactgtttc
    17401 gcgcatttct gaaagtggac gatcgttgtt cggtttgcgg actggatttc acgccgcacc
    17461 gcgccgacga tcttccggcc tatctcgtca tcgtcatcgt cggccacatc gtggtaccga
    17521 cgattttgtg gatcgaaacc gattatgcgc ctccggtgcc gttgcagctc gcgatctatc
    17581 tgccgttgac gctggtcttg tcgttgctgt tgctgcagcc ggtcaagggc gccgtggtcg
    17641 gggtgcaatg ggcattgcgc atgcacggat ttgacgaaca aaatcctgaa ccgtaatcgg
    17701 ggtcatacaa caagaaacaa aatggggcgg cgggagtgaa atgagcgaga ctgtaaagga
    17761 acagaaagaa gccgatcacc atccctatcg ccgccccgta gacgccgcca cgctgatcct
    17821 ggtcgatcgc gccgccatca ccccaaaagt tctggtcggc aaacgccacg acaaggtggt
    17881 attcatgccc ggcaaattcg tttttcccgg cggtcgcgtc gacaagacag acaatcgcat
    17941 tccggtagcc gcgcctatcc cgaaggcgct tgaaaccaat ctgatgaagg gcagtccgaa
    18001 gatcacgccg tcgcgcgcgc gctcactggc gatcgccgcg atccgcgagg cctgcgagga
    18061 aaccggcctc tgcctggggc gcaagaccga cggcgctgcg ccgaagctgg agggcgaatg
    18121 gaaacccttc agtgacgccg gattgctgcc ggatccgtcc ggactgtttt tgatcgcacg
    18181 cgcgatcacc ccgccgggcc gggtgcggcg tttcgatacc aggtttttca ccgcggacgc
    18241 gtcaagcatc gcccatcgcg tcgagggcgt gatccatgcc gaggcggaac tggtggaact
    18301 ggtctgggtc gagatcggtt caaaaccgct ggccgatctg catccgatga ccaggaatgt
    18361 cctgaacgaa ctcgaaaagc gtctcgccac cggcccgctg aatcacgacg ccgaggtgcc
    18421 gtttttccac ttctacggcg gcaagatgca caaggatgtc ttggccgaaa cgaggagcgc
    18481 tgtgtcatga ttcggttcac aaaattcctg ctcgcctccg tcgcgttgtg cagcctgctc
    18541 tcgctgcccg cccgtgcgga gctcgatgtg gccaaactga aaaccacgat cgaggcgtcc
    18601 gtcgagaacg agtatccaaa gctggatgcg ctctacaagg acatccacgc ccatcccgag
    18661 atcgccttcc aggaagtgaa gaccgccgca aagctcgccg ccgaaatgcg cgcgatcggc
    18721 tttgaagtca ccgagcaggt cggcaagacc ggcctggtcg cgatctacaa gaacggcgac
    18781 ggccccacca tcatggtgcg caccgagctc gatgcgctgc cgatggagga aaagaccggc
    18841 ttgccctatg cgagccacga caagaccaac tggcacgggc gcgagacccc cgtcgcccat
    18901 agctgcggcc atgacattca catggcgagc tgggtcggca ccgccaagac gcttgtcggc
    18961 ctgaaagacc aatggcacgg caccttgatg ttcatcgccc agccggccga ggaagagatt
    19021 gcgggcgcga aagcgatgtt ggacgacggc ctgttcaccc gctttccgaa gcccgacgtc
    19081 ggctttgcct tgcatgacgg cgcctccgcc tacggcaccg tctcctatcg ggtcggaatc
    19141 ggctcgtcga atcagga
//
LOCUS       DATNOI010000489        10986 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_244878,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000489 DATNOI010000000
VERSION     DATNOI010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10986
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244878"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2850
                     /locus_tag="VN968_19635"
     CDS             <1..2850
                     /locus_tag="VN968_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NahK/ErcS family hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HXL31483.1"
                     /translation="ELIERAMKTPEAVRAIEYVPSIGNFLTMTLLSFCAIMLLPRQFH
                     VSVVENSSDAEVGRARWLFPLYLVAINLFVIPIALAGLVTFPFGAVDSDMYVLALPMA
                     GDSPWLSVGVFVGGLSAATAMVIVECVALSIMVSNDIVVPLVLKRGAQTRAGQKDFGD
                     FVLRVRRFAIFAIMVMAYFYYRALGNTQLVAIGLLSFAAIAQLAPAFFGALFWRRATA
                     RGAMGGMLVGFAVWIYTLFLPSFLDGSTAGMLLLQHGPFGIEALRPQALFGADLPPLL
                     HGVLWSLSLNLATYVVLSLARRPSSIERLQADLFVPNALAPIAPTFRRWRTTVTVQDI
                     QSTVAQYLGPDRARHSFEAFAITHHTSLEPSAPADFELLQHAEHLIASSIGAASSRLV
                     MSLLLRKRTVSAKAALKLLDDSHAALHFNREILQTALNHVRQGIAVFDADLQLICSNR
                     QFGEILGLPPHLVQIGIPLREILEFMGAVDSPEVGDSDALLEKRLTAYTTEGEPYLER
                     LPDRHMVIEVRANRMPGGGLVITFFDVTPSFETAEALERSNATLEKRVRDRTEELTRL
                     NSELAQAKSTAEDANISKTRFLAAASHDILQPLNAARLYVTSLVERQNGGEDSRLVEN
                     IDDSLEAIEEILGALLDISRLDAGAMTTSITSFKIGDLMRSLEIEFTPMARAKALKLT
                     FVPCSLPVASDRALLRRLLQNLISNAIKYTPRGRVLVGCRRRGESLQIGVYDTGVGIP
                     VLKRGEIFKEFHRLEQGARIARGLGLGLSIVERLGRVLNHGIAIDSNASGGSVFSVTV
                     PIASAINHTTAVTSATPLSRTPMSGSLIVCIENDPAILDGMKTLLTAWDAEVIAVADP
                     DAAIEAIEAAGGRVTGLLVDYHLDRGNGVAAIRDIRRRFGDAIPAILITADRSPHVRA
                     AAREEQITILNKPVKPASLRALLGQWRTQQMVAAE"
     gene            complement(2883..3659)
                     /locus_tag="VN968_19640"
     CDS             complement(2883..3659)
                     /locus_tag="VN968_19640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31484.1"
                     /translation="MSRPPTVLSRRAALATLAAAAYASATAGSMVEGMAVENELGPVF
                     SPTGPDAELYGATEGFPIADRSLPIQPGNPYQPKYRVGAFSHFDEIYPTRRIKRAATP
                     WTFKRSQAEFRLSAGEGVAADRRGSRRDMAFGCGRARTGALRIQCGAARLRLGRLLAH
                     DGACQDKQIIPAQWMIDATTVRASDAYLLPGKAMPTFGYGYLLWLLPGTRRQFALVGD
                     LGQRICVDPDSKLVMVQTALETRAEVWRLWSAVVEQLGQR"
     gene            complement(3940..4605)
                     /locus_tag="VN968_19645"
     CDS             complement(3940..4605)
                     /locus_tag="VN968_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL31485.1"
                     /translation="MTSAASTHLVIADDHPLFRDALRQAVASVVTSARIDEAGSFDEL
                     TALLEQDSDVDLILLDLTMPGISGFSGLIYLRAQYPAIPVVIVSASDDGGTIRRSLDF
                     GASGFIPKRFGVETLRDAIVKIMDGDVWVPPDTDLTSAADPDMSRLRDRLVTLTPQQV
                     RVLMMLSEGLLNKQIAYELGVSEATIKAHVSAILQKLGVESRTQAVIAAAKIAGGQWR
                     PSA"
     gene            4959..6641
                     /locus_tag="VN968_19650"
     CDS             4959..6641
                     /locus_tag="VN968_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL31486.1"
                     /translation="MSTLAATEVRTGGMTKDERFVILASSLGTVFEWYDFYLYGSLAS
                     IIGAQFFSAYPPATRDIFALLAFAAGFLVRPFGAIVFGRVGDIVGRKYTFLVTIMIMG
                     LSTFLVGLLPGAAQIGFAAPVILISLRLLQGLALGGEYGGAATYVAEHSPQGKRGYYT
                     SFIQTTATLGLFLSLLVILFTRTWTGEAAFAGLPGEYWGGWRIPFLVSVILLGISVWI
                     RLRLNESPVFQKMKDEGKGSKAPLTEAFANWGNAKYVILALVGATMGQGVVWYTGQFY
                     ALFFLQSILKVDGYTANLLIAWSLLFGTGFFIVFGALSDKIGRKPIILAGCLIAALTF
                     FPIFRMISANANPTLEKAIEATKVEVLADPAGCGDLFNPVGTRVFSAPCDTVRAFLAQ
                     SSVKYSTVYGAAGSGVKVTVNGKEVPYTDAKTSNPTVAAAVAGAGYPKAGDPGIVKMS
                     NPFDIFRPQVAAIIGLLFILVIFVTMVYGPIAALLVELFPTKIRYTSMSLPYHIGNGW
                     FGGLLPATAFAIVASTGDIYAGLWYPIIFASITAVVGFFFLPETKDVDIKTN"
     gene            7298..7930
                     /locus_tag="VN968_19655"
     CDS             7298..7930
                     /locus_tag="VN968_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXL31487.1"
                     /translation="MSDALIGLIPTCGPWIIFGIVALESAGVPLPGETILVASALLAA
                     TTGQINIVLVVLAAAAGAIVGDGMGYLVGRRFGLPFLRRYGRYIRLDEDRLLIGRYLF
                     LRYGNAVVFFGRFIAVLRMFAALLAGANSMPAGRFFFFNITGGVCWACLFGFGAYAVG
                     AEIYKISGVLSMISLGLFIAAGYTLSIFVRRNEAVLRRRAEQALSGCSRG"
     gene            complement(8089..9159)
                     /locus_tag="VN968_19660"
     CDS             complement(8089..9159)
                     /locus_tag="VN968_19660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31488.1"
                     /translation="MQRYVLAMLLVGGVWLTGAYFTARAAPCLMVTLTGTQSGPAIFD
                     GIAGAGTLVRYGDDSNECGALKLQFDAGRGTILRLSQVGVVPGQLNATFFTHMHSDHT
                     EGFAEIAQLRWNFNSAGPKLDVVCSEDAISTLGFTLSCKKLVMHIGDLFIQSGEIAQR
                     ISEDEQRLPGGPVDILNIVTFQPTNEQQIVWTSGEVRVSAIRSTHIAGHASYRVDTPA
                     GSVVIGGDASNDTLEPPRLTSTSDQVEKLAKGADVIVHSTMHPAMAPDKGSGMPPPVY
                     YRQAATADLGAMAQRAGARYLMLTHMAPPLGAQRHGPYKVPGGPLTDADYRKVVEASG
                     FTGITIVGTDLATIRLPAKQAQ"
     gene            9649..9777
                     /locus_tag="VN968_19665"
     CDS             9649..9777
                     /locus_tag="VN968_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31489.1"
                     /translation="MNWFYWDTAWSLFFSLVWFMTITNNPDARIADAHLRDEAFGG"
     gene            complement(9854..10057)
                     /locus_tag="VN968_19670"
     CDS             complement(9854..10057)
                     /locus_tag="VN968_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31490.1"
                     /translation="MAHSVIYRDLNRPDAVIDEIVARCRGDMVSAVKALLLVNEELES
                     ELQQLYSAAACRPIKRQAKTSLH"
     gene            complement(10412..10882)
                     /locus_tag="VN968_19675"
     CDS             complement(10412..10882)
                     /locus_tag="VN968_19675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HXL31491.1"
                     /translation="MTSPRSLSRSPSSIVSINTIVSSILTIQTPGRGFVDLTAEIAKF
                     VKDAGAREGAVTLFIRHTSASLTIQENADPLVLDDLITALDRLAPEDAGWTHDTEGPD
                     DMPAHIKTVLTASSLHIPVLGGELALGTWQAIYLIEHRTRPHRREIVLQFAGAT"
BASE COUNT         1952 a         3368 c         3386 g         2280 t
ORIGIN      
        1 gagctgatcg aacgcgcgat gaagacgccg gaagcggtgc gcgccatcga gtatgtgccc
       61 tcgatcggca atttcctcac catgacgctg ctgtcgttct gcgcgatcat gctgctgccg
      121 cgccagtttc acgtcagcgt ggtggagaat tccagcgatg ccgaggtcgg ccgggcgcgc
      181 tggctgtttc cgctgtatct ggtcgccatc aacctgttcg tgattccgat cgcgctcgcg
      241 ggtctggtca cctttccgtt tggcgctgtc gacagcgaca tgtatgtgct ggcgttgccg
      301 atggccggcg attcgccgtg gctgagcgtc ggggtctttg tcggcggcct ctcggcggcg
      361 accgccatgg tgatcgtgga atgcgtcgcg ctgtcgatca tggtttccaa cgacatcgtg
      421 gtgccgctgg tgctgaagcg gggcgcgcaa acccgcgcgg gccagaagga ctttggcgac
      481 ttcgtgctca gggtccgccg gtttgcgatt tttgccatca tggtgatggc ctatttctac
      541 taccgcgcgc tcggcaatac ccagctggtg gcgatcggcc tgttgtcctt tgcggccatc
      601 gcgcagcttg cgccggcatt cttcggtgcc ctgttctggc ggcgcgcgac cgcgcgcggc
      661 gccatgggcg gcatgctggt cgggttcgcg gtgtggatct atacgctgtt tctgccgagc
      721 tttctggacg gcagtaccgc cggcatgctg ctgctgcagc acggcccgtt cggcatcgag
      781 gcgttgcgcc cgcaggccct gttcggcgcc gatttgccgc cgctgctgca cggcgtgctc
      841 tggtcgctct cgctcaacct tgcgacctac gtcgtgctgt cgctggcgcg ccggccgtcc
      901 tcgatcgaac ggttgcaggc ggacctgttc gtccccaatg cgctggcgcc gattgcgccg
      961 acattccggc gctggcgcac cacggtgacc gtgcaggaca ttcagagtac ggtggcgcag
     1021 tatctcggtc ccgatcgcgc ccgccattcg ttcgaagcgt ttgccattac ccaccacacg
     1081 agcctcgaac cgtcggcgcc ggccgacttc gagctgctgc agcatgccga gcatctgatc
     1141 gcttcctcga ttggcgcggc gtcctcgcgc ctggtgatgt cgctattgct gcgcaagcgt
     1201 accgtctcgg ccaaggccgc cctcaagctg ctggacgact cccacgccgc attgcatttc
     1261 aatcgcgaga tattgcagac cgcgctcaac catgtgcggc agggcatcgc ggtgttcgac
     1321 gcggatttgc agctgatctg ttcgaaccgg cagttcggcg aaattctcgg gctgccgccg
     1381 cacctcgtgc agatcgggat tccgctgcgc gaaatcctcg aattcatggg agcggtcgat
     1441 tcgcctgaag tcggcgacag cgacgccctg ctggagaagc ggctgacggc ctataccacc
     1501 gagggcgagc cttatctgga acgcttgccg gatcgacaca tggtgatcga ggtgcgcgcg
     1561 aaccggatgc ctggcggcgg actggtcatc acgtttttcg acgtcacgcc atcgtttgaa
     1621 acagccgaag cgctggagcg ctccaacgcc accctggaaa aacgcgtgcg cgatcgcacc
     1681 gaggaactca cccgcctgaa ttcggaactg gcgcaggcca agagcaccgc ggaggacgcc
     1741 aacatctcga aaacccggtt cctggcggcg gcaagccacg acatcctgca accattgaac
     1801 gcggcgcggc tctacgtgac gagcctcgtc gagcgccaga acggcggcga ggattcgcgg
     1861 ctggtcgaaa acatcgacga ttcgctggag gcgatcgagg agatcctcgg cgcgctgctg
     1921 gatatttcgc ggctcgacgc cggcgccatg acgacgtcga tcaccagctt caagatcggc
     1981 gacctgatgc gttcgctgga aatcgaattc acgccgatgg cacgcgccaa ggcgctgaag
     2041 ctaactttcg tgccgtgttc gttgccggtg gcatccgatc gcgcgttgct gcggcggctg
     2101 ctgcagaacc tgatctccaa cgccatcaaa tataccccgc gcggccgcgt gctggtcggc
     2161 tgccggcgcc gcggcgaatc gctgcaaatc ggcgtatatg ataccggcgt cggcattccc
     2221 gtgctcaagc gcggcgagat cttcaaggag tttcaccggc tggaacaggg cgcccggatt
     2281 gcccgcggcc tcgggcttgg gctgtcgatc gtcgagcgtc tcggccgcgt cctcaaccac
     2341 ggcatcgcga tcgattccaa tgccagcggc gggtcggttt tttcggtcac ggtgcccatc
     2401 gccagcgcca tcaatcacac cacagccgtc accagcgcga ccccgctttc gaggacgccg
     2461 atgagcggct ccctgatcgt gtgcatcgaa aacgatccgg ccatcctgga cggcatgaag
     2521 acgctgctga cggcctggga tgccgaggtc atcgcggtcg cggatccgga tgccgccatc
     2581 gaggcgatcg aggccgcggg cgggcgggtc accggtcttc tggtcgacta tcatctcgat
     2641 cgcggcaacg gcgttgccgc gatccgcgac attcgccgcc ggttcggcga tgccattccg
     2701 gcgatcctga ttaccgcgga tcgcagtccg catgtgcggg ctgccgcccg ggaggagcaa
     2761 atcacgatcc tcaacaagcc ggtcaagccg gcatcgctgc gggcgttgct gggccaatgg
     2821 cgcacccagc agatggtggc cgccgaatag ctgcggaagt gaggatgatc gtaggcgcgg
     2881 cgtcatcgct ggccgagttg ttccaccacg gccgaccaca gccgccagac ctcggccctg
     2941 gtctcaagcg cggtctggac catgacaagc tttgagtccg ggtcgacgca aatgcgttgg
     3001 cccaagtcgc cgacgagcgc gaactggcgc cgcgtgcccg gaagcagcca caacagatag
     3061 ccatagccga acgtcggcat ggctttgccc ggcagcaagt aggcgtcgga ggcgcgcacc
     3121 gtcgtggcgt caatcatcca ttgtgctgga atgatctgct tgtcttggca tgcgccgtca
     3181 tgggcaagca ggcgtccgag ccgtagtcgc gcagcaccgc attgaatccg aagtgcgcca
     3241 gttcgagccc ttccgcatcc aaaagccatg tcgcgtcggc ttccgcgccg atcggctgcc
     3301 acaccttctc ctgcagataa tcggaattcg gcttgcgaac gtttgaacgt ccatggcgtc
     3361 gcggcgcgct tgatccgccg cgtcggatag atttcgtcga aatggctgaa cgcgccgaca
     3421 cgatatttgg gctgataggg gttgccgggt tgaatcggga gcgagcgatc ggcgatcgga
     3481 aagccttccg tagctccata aagctcggcg tcgggaccgg tgggcgaaaa caccggacca
     3541 agctcgtttt cgaccgccat gccctcaacc atcgatccgg ccgtggcgga ggcatatgcg
     3601 gcggcggcca gcgttgcgag cgccgcgcgg cgagacaaaa cggttggcgg cctcgacatg
     3661 cgatttcccc tgccgccgtg gatagggcga atacccattg gcgcgtggtt ctcctatagt
     3721 ctcgtcggcg acggaggatc gcaatcccga acgccagggt cgagctgctt gctggcgtca
     3781 gtcattacga cttcctgtcg atgtgcaccg acaccgggcg cgcgatcagt cccgcctgcg
     3841 agcgcgcgca cgcccaggct gatacgcacc gtcgagcaat cgcggcggcg gaatctttct
     3901 tcggccgata ccttttgggt tctcaatgac ttcagcgcgt catgcgctgg gccgccactg
     3961 gccgccggcg attttcgccg cggcgatcac cgcctgggtg cggctttcga cgccgagctt
     4021 ctgcaggatc gccgagacat gcgccttgat ggtcgcttcc gacaccccaa gctcgtaggc
     4081 gatctgcttg ttgagcaggc cttcggacag catcatcagc acccgcacct gctgcggggt
     4141 cagcgtcacc aggcggtcgc gcaggcggct catgtccgga tcggccgcgg aggtgagatc
     4201 ggtatccggc ggaacccaga cgtcgccatc catgattttc acgatcgcgt cacgcagcgt
     4261 ctcgaccccg aaccgcttgg gaatgaaccc ggaggcaccg aaatccagcg agcggcggat
     4321 ggtgccgccg tcgtcgctgg ccgatacgat gaccacggga atcgccggat attgcgcgcg
     4381 caggtaaatc aggccggaga aaccgctgat gccgggcatc gtcagatcga gcaggatcag
     4441 gtcgacatcg gaatcctgct caagcagcgc ggtcagttca tcgaacgagc ccgcttcgtc
     4501 aatcctggcc gaggtcacga cactcgcgac cgcctgtcgc aacgcatcgc gaaacagcgg
     4561 atggtcatcg gcaatgacga gatgcgtgct ggcggcggag gtcatttatc tcctgcgaaa
     4621 gggttgcggc caagccgtga tcgcgtttcg tagagcgcgc gccaggcgaa ttctttgtcg
     4681 caattgtcct ctgagcgatg cttgcatgca agcgccctgt attgctatgc cgcaaagcgg
     4741 ttaatcgccc gatttgtgcg ccgcaatacg cccgttccca aagcattttc gggcgatcga
     4801 agcgcggact tctgggcata gccgtgcaaa gggccaaaag tcttattggg ggaggtttca
     4861 cggcgatgtt accgtgaacc gaagcctgta gctgtaatcc ggcccagttc ggggcatcag
     4921 gcgatctttg catcaacgat ttcggggagc gatccaaaat gtccacattg gctgcaacgg
     4981 aagtcagaac cggaggcatg acgaaggacg aacgtttcgt cattctcgcg tcatcgctcg
     5041 gtaccgtttt cgaatggtat gatttctatc tttacgggtc gctcgccagc atcatcggcg
     5101 cgcagttctt ctcggcctat ccgccggcga cgcgcgatat cttcgcgctg ctcgcctttg
     5161 ccgccggctt cctggtgcgc ccgttcggcg ccatcgtgtt cgggcgcgtc ggcgacatcg
     5221 tcggacgcaa atacaccttc ctggtcacca tcatgatcat gggcctgtcg acgtttctgg
     5281 tcggccttct gcccggcgcc gcgcaaatcg gctttgccgc gccagtgatc ctgatctcgc
     5341 tgcgcctgtt gcagggcctt gcgctgggcg gcgagtatgg cggagcggct acttatgtcg
     5401 ctgagcactc gccacagggc aagcgcggtt attacacctc gttcattcag accacggcca
     5461 cactcggctt gttcctgtcg ctgctggtga tcctgttcac ccgcacatgg accggtgaag
     5521 ccgctttcgc gggtcttccc ggcgagtact ggggcggctg gcgcattccg ttcctggtgt
     5581 cggtcatcct gctgggaatc tcggtatgga tccggctgcg gctcaacgaa tcgccggtgt
     5641 tccagaagat gaaggacgaa ggcaagggct ccaaggcccc gctgaccgaa gccttcgcca
     5701 actggggcaa tgccaaatac gtcatcctgg cgctggtcgg cgccaccatg ggtcagggcg
     5761 tggtctggta taccggccag ttctacgcgc tgttcttcct gcaatcgatc ctgaaggtcg
     5821 acggctacac cgccaacctt ctgatcgcct ggtcgctgtt gttcggcacc ggcttcttca
     5881 tcgtgttcgg ggccttgtcg gacaagatcg gccgcaagcc gatcattctg gccggctgcc
     5941 tgatcgcggc gctgaccttc ttcccgatct tccggatgat ctccgccaac gccaacccga
     6001 cgctggaaaa ggccatcgaa gcgaccaagg tcgaagtgct ggcggatccc gccggctgcg
     6061 gcgatctgtt caatccggtc ggcacccgcg tgttctccgc gccctgcgat accgtccgcg
     6121 ccttcctggc tcagtcgtcg gtcaagtatt cgaccgtgta cggcgccgcc ggctcaggcg
     6181 tgaaggtcac cgtcaatggc aaggaggtgc cctataccga cgcgaagacc agcaatccga
     6241 ccgtagccgc ggctgttgcc ggggcgggct atccgaaggc cggcgatccg gggatcgtga
     6301 agatgtcgaa cccgttcgac atcttccgtc cgcaggtcgc ggcgattatc gggctgctgt
     6361 tcatcctggt gatcttcgtc accatggtgt acgggccgat cgcggcgttg ctggtcgaac
     6421 tgttcccgac caagatccgc tacacctcga tgtcgttgcc ctaccacatc ggcaacggct
     6481 ggttcggcgg actgttgccc gcgaccgcat tcgcgatcgt ggcctcgacc ggcgatatct
     6541 atgccggcct gtggtatccg atcatcttcg catcgatcac cgcggtggtc ggcttcttct
     6601 tcctgccgga aaccaaggac gtcgatatca agacgaactg acggttttgc gagcggccaa
     6661 accgctcgga caaaacccgc tcaacgaacc ggccgcggag cgatccgcgg ccggttttgt
     6721 atttattgga gactagagcg cgatgacttt cttcgaatcg tcatagctac gccgacatcg
     6781 cgtttcggag cgattaaccg caaagcggtt atctcgttcc agcgcacctc gccctcgctc
     6841 gcgagccatc gcgatgaatt caatcacatc aattttgcgg caccccatac cgcctgacgc
     6901 gcaaccgtcg gaactccgca ggcgaccgcg accttgcgcc aacatcatta cggcgaatta
     6961 actggatact catctggcat tcagcttgga acttctacgg ttctaccggg agttgaggtc
     7021 cagaggagaa tgcggtgaaa cttacgcatt cccctgtcgg actggtggca actcggcggc
     7081 gcagcggcga gcattggagc cgccggctgg cttagccatc aacaccgacg tcgcttcaaa
     7141 acattgtgcg ggcccgcatt gtttgctgcc gttggggtcg ttgctggaac gtacccggct
     7201 acggcggctc ccatggaccg cgatatgatg atggctatgc tacggttggc gccgcgggcc
     7261 ggcgcagcct tccttttaga agatggagta tacttctttg tccgatgctt tgattggctt
     7321 gatcccaacc tgcggcccgt ggatcatctt tggtattgtt gcattggaaa gtgccggcgt
     7381 gcctctcccc ggagagacga tcctggttgc cagtgcgctt cttgccgcga ccactggtca
     7441 gatcaacatc gtcctcgttg tactagccgc tgcggcaggt gcgattgtcg gggacggtat
     7501 gggatacctg gtcggacgtc ggtttggttt gccctttctt cgccgatacg gtcggtacat
     7561 ccgcctcgat gaggatcgat tgctgatcgg tcgatatctt tttttgcggt atgggaacgc
     7621 cgtcgtgttc tttgggcgtt tcatcgcagt gctgcgaatg tttgcggctt tgcttgcggg
     7681 cgccaatagc atgccggcgg gtcgtttttt cttcttcaat ataaccggtg gagtctgctg
     7741 ggcgtgtctc ttcggcttcg gtgcttatgc agtgggcgcc gaaatctaca agatttcggg
     7801 agtgctgagc atgatctcat tgggcctgtt tattgcggca ggatatacgc tctcaatttt
     7861 cgttcgacga aacgaagctg tgctgcgccg tcgcgcggag caagccctgt caggttgttc
     7921 gcgcggatga gccatatcag atcatggcac tttcggacct ggcgcgatga gcgaagccct
     7981 cgcccgagac cggtccagaa ggtgaccgcg ggagcgttct ccaattgcgg gcgagggtca
     8041 accgttctct gcgtgcgccg cgattcataa ttgcgctccg ggaaagactt attgcgcctg
     8101 tttcgcagga agacggatag tcgccagatc ggttcccacg attgtgatac ccgtgaaccc
     8161 gctcgcctcg acgacctttc gatagtccgc gtctgtaagt ggtccgccgg gtaccttgta
     8221 aggaccgtgc cgttgcgccc cgagcggcgg agccatgtgc gttaacatca aataccttgc
     8281 gcccgcgcgt tgcgccattg cgccaagatc ggccgttgcg gcctgacgat aatagaccgg
     8341 cggtggcatg ccactgccct tgtcaggcgc catggcggga tgcatcgtcg aatggacgat
     8401 gacatctgcg cctttcgcta acttctcaac ttgatcagag gtcgaggtga ggcgcggggg
     8461 ttccagcgta tcgttgctgg catcgcctcc tatcaccacg ctgccggccg gggtgtcgac
     8521 ccgatacgat gcatgacctg cgatgtgtgt cgagcgaatg gcgcttaccc tgacctcgcc
     8581 ggaggtccac acgatctgct gttcgttggt cggctggaat gtgacgatgt tgagaatatc
     8641 gacaggacct cccggcaatc gctgctcgtc ttcggagatg cgctgggcga tttcgccgga
     8701 ttgaatgaag agatctccta tatgcatcac gagtttcttg caactcagcg tgaagccgag
     8761 cgtcgatatg gcgtcttcgc tgcaaacgac gtcgagcttc ggtcccgccg agttgaaatt
     8821 ccagcgcaat tgagcgattt cggcgaaacc ttcggtatga tccgagtgca tatgcgtgaa
     8881 aaacgttgca ttgagctgcc ccggcacaac gccaacttgc gatagtcgca ggatggtccc
     8941 tcggcccgca tcaaattgca gcttcaaggc accacattcg ttgctgtcat cgccgtaacg
     9001 caccagcgtc cctgctcccg cgatgccatc aaagatggcg gggccgctct gcgtgccagt
     9061 caatgtcacc atgaggcagg gggctgctcg cgcagtaaaa tatgcgcccg tcaaccacac
     9121 gccgccaacg agtagcatcg caaggacgta tcgttgcatg catgcctctt ggcggatctt
     9181 gggaactggg cggtagattg tccggttctg caaaggcgag caagaccgcc tctgccactt
     9241 ttagggcttg gcgcgatgtc tgattggatt ccgtcctgcg ctccaggcgg acttcgacaa
     9301 tatgcgccgc gaccagcgac gattggcgcg aacaccacag cggctgttca aaagcacgtg
     9361 aaattgaacc gaggccgccc gatggggtcg gcaggcggag accggtgacg ccctggatta
     9421 acggctgacg cgcccctaaa agcgttggcg taactgcgaa aatgcgtcga cgacaccaag
     9481 ggatcggatg acggacgccg tcaagccctc cgccgggcgg tcaggttgaa caggttgccg
     9541 ccgctgcgcg tcgcaatacc gcagcgcaaa tgcaccgaat cctcgggacg ctttcgccga
     9601 atgctgatag ccttcctgct ctcgacggtc cttccgcggg gaaaccacat gaactggttc
     9661 tattgggaca cggcatggtc gctgttcttc agcctcgttt ggttcatgac catcacgaac
     9721 aatcccgacg cgcggatcgc ggatgcgcat ttgcgcgatg aagcattcgg cggctgaatc
     9781 aagttccacg tcagcgaacg gatcgggctg aagcaagcca gacgcccgaa gggccatctg
     9841 ccgcggacaa aagtcaatgt agcgaagttt tcgcctgacg tttgatcggg cggcaagctg
     9901 ccgccgagta aagctgttga agttcggact ccagttcctc gttgacgagc agcagagctt
     9961 tcactgcgct gaccatgtcg ccgcgacagc gggctacaat ctcatcgata acggcgtcgg
    10021 gacgattcag gtcgcggtag attacggagt gagccatggt aacatcctcc gactgaaaga
    10081 gctgagctac ataaagagct gagctacatg ctacacctag tccagcgtcc agcacgtatg
    10141 ctagtccagc gtccagcacg tatgccgggc tatgcccgct attcacaggg cggaaccagc
    10201 aaatattatt taccgggcct ctgaaaatct gcgccgaaca cgcgctcctc gtgacggttg
    10261 gggcgtccgc tcgaaaacac cctagcgcgc gatgactttt cttcgaatcg tcatcccgct
    10321 ctatcttttt gtttgagcat gatcttttcg gaaaaccggt aaccaccctc gggtcaagcc
    10381 cgagggcagg cttttccgga tcatgctctg gctaggtcgc gcccgcgaac tgcagcacga
    10441 tctcgcgccg gtgcggacgc gtgcggtgct cgatcaggta gatcgcctgc catgttccca
    10501 gtgcgagttc gcctccgaga accgggatgt gcagcgaact cgccgtcagc acggtcttga
    10561 tatgggccgg catgtcgtcg gggccttcgg tgtcgtgggt ccatccggcg tcctcgggcg
    10621 ccaaccggtc gagcgccgtt atcaaatcgt cgagtaccaa cggatcggca ttttcctgga
    10681 tcgtcagcga cgccgaggtg tggcggatga acagcgtcac cgcgccctcg cgcgcgccgg
    10741 cgtccttgac gaatttggcg atctcggcgg tgagatcgac gaagccgcgc ccgggggtct
    10801 ggatggtcag gatcgacgac acgatggtgt tgatgctgac gatcgaagac ggagagcgtg
    10861 agagcgatct aggtgaagtc ataaaatatc tctcgatgcg tcgtcccggc gaacgccggg
    10921 acccataacc cctggcgatc attattgcga aaggcctttg ctgcaatgcc caagcgcgaa
    10981 gacacg
//
LOCUS       DATNOI010000490         2440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_195640,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000490 DATNOI010000000
VERSION     DATNOI010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195640"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VN968_19680"
     CDS             complement(<1..765)
                     /locus_tag="VN968_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HXL31492.1"
                     /translation="MHVFSVPVSAPFLRTVIAALVDGRLIQGFEPRARPAELAQATLY
                     LPTRRAGRMAREIFLDELKADAVVLPRIVALGDIDEDELAFAEQAEPYGGVAPLDIPP
                     KLGELDRRLTLAGLVAAWAKGPVLAPLVVGGPASTLALAGDLARLMDDMVTRGVGWEA
                     LDGLVPDQLDEYWQHSLEFLRIAQRNWPAHLQEIGKLEPAARRDLLIAAEAARLTTHH
                     DGPVIAAGSTGSMPATAKFLHAVAALPQGAVVLPGLD"
     gene            1287..>2440
                     /locus_tag="VN968_19685"
     CDS             1287..>2440
                     /locus_tag="VN968_19685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HXL31493.1"
                     /translation="MRHLTVYNRFAMIIAVLTVVFVAALAAQVMVLRNTVIEERRTKV
                     FDLVEAAKKILANYEEKAKAGKISAEQARQLAFDAIGAMRWGKSADYLGIYGAGSTNA
                     GVTYVHANPKYINVNRWDYKDNQGQLLIQNIVGKARTGGGYLTYQVPKATGGPELPKL
                     TYAGGYGEGDKLLAIQAGVYTDDIDAVVFDRAIWIAAGGLAGLLVAGLAAFALGRGLV
                     RPLGAICGVMDGLAKGDLSVEVPFVEHRSEIGHISRSLAVFKDRLIDAERLRGEREEA
                     TARAVAERKSVMNRIAREFEKSVGGIVAGAASAATEMQHSAQSLSAIAKETTRQSTTV
                     AAAAQQTTANVQTVAAAAEELATSGQEISRQIVYSASIAQSAVAQADRTNA"
BASE COUNT          457 a          749 c          789 g          445 t
ORIGIN      
        1 atcgaggccc ggaagcacca ccgcgccttg cggcagagcg gcaacggcgt ggaggaattt
       61 tgccgtggcc ggcatcgatc cggtcgagcc tgcggcaatc accggaccgt cgtgatgcgt
      121 ggtcaggcgc gcggcctccg ctgcgatcaa caggtcgcgc cgcgccgccg gctcgagctt
      181 gccgatttcc tggagatgcg cgggccagtt ccgttgcgca attctgagga attccagcga
      241 gtgctgccag tattcgtcca gttgatccgg caccaacccg tcgagcgctt cccacccgac
      301 gccacgcgtg accatgtcgt ccattagccg cgccagatcg ccggccagcg ctagcgtcga
      361 tgccggcccg ccgaccacca gtggcgctaa taccgggcct tttgcccacg ccgcgaccag
      421 ccctgccagc gtgagccggc ggtcgagttc gccgagcttc ggcggaatgt ccagcggcgc
      481 caccccgccg tatggctcgg cctgttccgc gaacgccagt tcgtcttcgt cgatgtcgcc
      541 gagcgcgacg atgcgcggca gcaccaccgc gtcggccttc agctcatcga gaaagatttc
      601 ccgcgccatg cgtccggcgc gccgggtcgg cagatagagc gtcgcctgcg ccagctcggc
      661 ggggcgcgcg cggggctcga atccttggat cagcctgcca tcgaccaacg cggcaatgac
      721 ggtgcgcaag aatggcgcgg atactggaac gctgaaaacg tgcatgggct tcctgattcg
      781 cgattctgcg catcatggca tgggcggcac cggggaggga gtcgccgagg gtcgcgccgt
      841 cattccgggg cgacgcgaag cgtcgaaccc ggaatctcga gattccgggt tcgcgtcttc
      901 gacgcgcccc ggaatgacga agcgtcagcc cgtagatggg gcaccggggc ccgttacccc
      961 atctacgctt ggcgggcgcg agatggtccg cctgatgtcg caaacgggtc aaaagggacg
     1021 gttcggcgat gttccgcgtt tcttgcgctt tgcccttgaa agccgacatt cattggaaga
     1081 gtcggcatgt ctcaaatgtg ccactggcgg aaagtggcct gtccataaaa ccgcccgggc
     1141 ctttatggcg tcaatgcttg ccggggaaca tgaaaacagc ctgaccttaa gcgtcagggt
     1201 tttcctcccg gcatgatcgt ctcaataaaa gcttaactgg taaagaaata ctgtcgccga
     1261 tcgcttctgt cacgaaggcg ctcatcatgc ggcatcttac cgtctacaat cgttttgcga
     1321 tgatcatcgc ggttttgacc gttgtgttcg tcgccgcgct ggccgctcaa gtgatggtcc
     1381 ttcgcaatac cgtcatcgag gaacgtcgga ccaaggtgtt cgatctggtc gaggccgcga
     1441 agaagatcct ggcgaattac gaggaaaaag ccaaagccgg caagatctcg gcggagcaag
     1501 cacggcagct tgccttcgat gcgatcggcg caatgcgctg gggcaaatcc gctgactatc
     1561 tcggaatcta cggcgcgggc agtaccaatg ccggcgttac ctatgtgcac gccaacccga
     1621 aatacatcaa tgtcaatcgc tgggattaca aagacaatca gggccagctg ctgattcaaa
     1681 atatcgtcgg caaggcccgc accggcggcg gttacctcac ataccaggtg ccgaaagcga
     1741 cgggtggtcc cgagctgccg aagctcacct atgccggcgg ctatggtgag ggcgacaagc
     1801 tgctcgcaat ccaggcaggc gtctataccg acgatatcga cgcggtggtg ttcgatcgcg
     1861 ccatctggat cgccgcgggc ggtctggcgg gattgctggt cgcgggattg gccgcattcg
     1921 ctctcggtcg cggcctggtt cgcccgctcg gtgcgatatg cggcgttatg gatggtctgg
     1981 cgaagggtga cttgagcgtt gaggtcccgt tcgttgagca tcgtagcgag atcggccata
     2041 tctcacgcag cctggccgtt ttcaaggatc ggttgattga cgccgaacgt ctccgcgggg
     2101 agcgggagga ggcgacggct cgcgccgtgg cggaacggaa atcggtgatg aaccggatcg
     2161 cgcgcgagtt cgagaaaagt gtcggcggaa ttgtcgccgg ggccgcttcg gccgccaccg
     2221 aaatgcagca ctcggcgcaa tcgctgtccg caatcgcgaa ggagacgacg cggcagagca
     2281 ccaccgtcgc cgcagccgcg caacagacca cggcgaatgt gcagaccgtg gctgccgcgg
     2341 cggaggaact tgcgacgtcg gggcaggaaa tctcccgtca gattgtttac tcggcatcga
     2401 tagcgcaaag cgccgttgct caggccgacc gcaccaatgc
//
LOCUS       DATNOI010000491         5393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1686665,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000491 DATNOI010000000
VERSION     DATNOI010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686665"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VN968_19690"
     CDS             complement(<1..306)
                     /locus_tag="VN968_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31494.1"
                     /translation="MSLGLSRDDRPILIAALFILVILVAGTTYTIARFGSAPLLSPTY
                     LLQQLQIGSFLGIVAAGMMIVILIAQIDLSVPWTLAAAAMMATSIGGPLAIPVGLGIG
                     "
     gene            complement(312..1424)
                     /locus_tag="VN968_19695"
     CDS             complement(312..1424)
                     /locus_tag="VN968_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31495.1"
                     /translation="MLDDLAIRLRQNIGFVTPIVLFCVFYLLYNLAHPKGFSSAVLVQ
                     NGDEIFALAMLAMAQTVPVLMSGLDLSVGAVMTLVGCFASYLLAGTAGGTPLHLEIFG
                     LHLGLGTFPGGTSGILLGIAACVAIGTLAGFINGCVVVYGRIQPIIATLATGAVYIGI
                     ALFLRPTPGGKIDEDLNWALTNSLGDFASTVHIFDDGAAGWFAPFAWIPVPFVLLVLI
                     ALFVWMPFRRSVLGRAVYAVGSAEGAAYMSGLPIERAKIAAFTLAGFFAGCGGLFLAI
                     QTSSGNADIPQAGAYTLNSIASVVIGGTSLLGGTGSAIGSIFGAMILRVISFFFRIFD
                     IAPLLQPLFEGVILLVAVSIGALGVLRVRNTLELFR"
     gene            complement(1417..2976)
                     /locus_tag="VN968_19700"
     CDS             complement(1417..2976)
                     /locus_tag="VN968_19700"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HXL31496.1"
                     /translation="MQDAVPTQPTFLTLSGISKRYGGVRALEGVDFACERGKIHAVLG
                     ENGAGKSTLIKIIAGVVQPDAGGMRLAGSDVSFATPSAANAAGVVCIFQELSLMPDLS
                     VADNISITSPPRRFGLIDARAQRRRAEALLAEIGCEDVNPLMRVRDLPLSRRQMVEIA
                     KALGQRPRLLILDEATSALTSADVEKVYAMLAKLKSEGIAILYISHRMHEVEALADRA
                     SVFRNGRHIETFDKGVRSTTDIVQLMIGRDITTQYPAKPARRPVKPVLALENLSWEKR
                     LHRISLGVGAGEIVGLGGLDGQGQKSLLLALFGVLRGVTGRITVEGREVRPGSPAAAK
                     SPAVGIALVPEDRKTEGLMLPMSIADNLAIASLDAISTGPFVDRAKERAAVERGIARL
                     QIKIGARSDAVSTLSGGNQQKVVIAKWLMTEPRIILLNDPTRGIDVGTKQELYRLMRE
                     LADQGAAILFYSTDYDELIGCCDRVAIMYDGRIVRELEGSELTETNIIASALNIDAAT
                     SAYAAAGAAHA"
     gene            complement(3042..4172)
                     /locus_tag="VN968_19705"
     CDS             complement(3042..4172)
                     /locus_tag="VN968_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HXL31497.1"
                     /translation="MKWTLGLTRVACASLLLSSVSAWAGPKIVAGPGANPGCFKPWSD
                     QTKFFQWDKKPGPYKIALVNGFVGNTWRIQMVKTAKAFAEQAGIKENIKEFKVVSTGT
                     DVAAQLGAMEDFINQGFDAIITIAVAPDGFDRIIRLADKNNVVVVPFDNILDTDKVMM
                     VNEDQKEMGRMSAKWLIDESGKKAGDILEVRGLPGNSVDRDRHIGFREIMEGAGNKFN
                     ITEVVGNWDTGTSQKVTADALAVHGHFDGVFTQGGSDGTVQAMVAAKHAFVPMSGEGE
                     NEFRKQIADHAKDGLKGMSYGQSPALVAIATKAAISALQGNVMPQLISIPIPVATYKD
                     LKPGTNYWPDLSANFFAPNQFLPCGVNFTAPEIMSQSEKNTQ"
     gene            4471..5082
                     /locus_tag="VN968_19710"
                     /pseudo
     CDS             4471..5082
                     /locus_tag="VN968_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004670754.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
     gene            5085..5171
                     /locus_tag="VN968_19715"
                     /pseudo
     CDS             5085..5171
                     /locus_tag="VN968_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003579903.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor"
BASE COUNT         1039 a         1650 c         1778 g          926 t
ORIGIN      
        1 gccgatgccg agcccgacgg gaatcgcgag cggcccgccg atcgacgtcg ccatcatggc
       61 ggcggccgcc agcgtccagg gcaccgacag atcgatctgg gcgatcagga tgacgatcat
      121 catcccggcc gccacgatgc caaggaacga gccgatctgc agttgctgca gcagataggt
      181 cggcgacaat aacggagcgc tgccgaaacg ggcaatcgta taggtggtgc ccgccaccag
      241 gatgacgagg atgaacaggg ccgcgatcag gatcgggcgg tcgtcgcgcg agaggccgag
      301 gctcatcgtc gtcatcggaa cagctccagc gtattcctga cgcgcagtac gcccagcgcc
      361 ccgatgctga ctgccaccag caggatcacg ccctcgaaca gcggctgcag caagggggcg
      421 atgtcgaaga tgcggaagaa gaaggaaatg acgcgcagga tcatggcgcc gaagatcgag
      481 ccgatggcac tgccggtgcc gccgagcagc gaggtgccgc cgatgacgac cgaggcgatg
      541 gagttgagcg tataggcgcc ggcctgcgga atatcggcat tgcctgatga ggtctggatg
      601 gcaaggaaaa ggccgccgca gccggcgaag aagccggcga gcgtgaaagc cgcgatcttg
      661 gcacgctcga tcggaagccc cgacatatag gctgctcctt cggccgagcc gaccgcatag
      721 accgcgcggc ccaggacaga gcgccgaaac ggcatccaga caaagagcgc gatcaggacc
      781 aggagcacga agggcaccgg aatccaggcg aagggcgcaa accagcctgc cgcgccatcg
      841 tcgaagatgt gcaccgtcga ggcgaaatcg ccgagcgaat tcgtcaacgc ccagttgaga
      901 tcctcgtcga tcttgccgcc gggggtcggg cgcaggaaca gcgcgatgcc gatatagacc
      961 gcgccggtag cgagcgtcgc gataatcggc tggatgcgtc catagacgac gacgcagcca
     1021 ttgatgaagc cggcgagcgt gccgatcgcg acgcatgcgg cgatgccgag caggataccg
     1081 gaggtgccgc ccgggaaggt gccgaggcca agatgcaggc cgaaaatctc gagatgcagc
     1141 ggcgtgccgc ctgccgtgcc ggccagcaga tagctcgcga agcagcccac cagggtcatc
     1201 acggcgccga ccgacagatc gagccccgac atcagcaccg gcacggtctg ggccatggcc
     1261 agcatagcga gcgcgaagat ctcgtcgcca ttctgcacca gcacggccga ggagaagccc
     1321 ttcggatgtg ccaggttgta gagcagatag aagacgcaga acaacacgat gggggtgacg
     1381 aagccgatat tctgacgcag cctgatggcg agatcgtcaa gcatgggcgg ccccggctgc
     1441 ggcataggcc gatgttgcgg catcgatgtt gagggcactc gcgatgatgt tggtctcggt
     1501 gagctcggag ccttcgagct cacgcacgat gcgcccgtca tacatgatgg ccacccggtc
     1561 gcagcagccg atcagctcgt cataatcggt cgagtagaac aggatggcgg cgccctggtc
     1621 ggcgagctcg cgcatcagcc ggtagagctc ctgcttggtg ccgacatcga taccgcgcgt
     1681 cggatcgttg agcaggatga tgcgaggctc ggtcatcagc catttggcga tcaccacctt
     1741 ctgctggttg ccgcccgaca gggtcgagac cgcatcgctt cgtgcgccga tcttgatctg
     1801 cagccgggcg atgccgcgct cgaccgccgc gcgctccttg gcgcgatcga cgaaaggccc
     1861 ggtcgaaatg gcatcgagcg atgcgatcgc cagattgtcg gcgatcgaca tcgggagcat
     1921 caatccttcg gtcttgcgat cctcgggcac cagcgcgatg ccgaccgcgg gcgacttcgc
     1981 cgcggccgga gagccggggc gcacctcgcg gccctcgacc gtgatgcgcc cggtgacgcc
     2041 gcgcagcacg ccgaacaagg caagcagcag cgatttctgg ccctgcccat cgaggccgcc
     2101 gaggccgacg atctcgccgg cgccgacgcc gagcgaaatg cggtggagac gcttctccca
     2161 ggacaggttc tcgagtgcca gcaccggctt caccggccga cgcgccggct ttgccggata
     2221 ttgggtggtg atgtcgcgcc cgatcatcag ctgaacgata tccgtggtcg agcgcacgcc
     2281 cttgtcgaag gtttcgatat ggcgtccgtt gcggaacacc gaggcccggt cggcgagcgc
     2341 ctcgacctcg tgcatgcggt gcgagatgta gaggatggcg atgccctcgg atttgagttt
     2401 cgcgagcatc gcatagacct tctccacgtc ggcgctggtg agagccgagg tggcctcgtc
     2461 gaggatcagg agccgcggcc tttggccgag agccttggcg atctcgacca tctgacgccg
     2521 cgacagcggc aggtcgcgca cccgcatcag cgggttgacg tcctcgcagc cgatctcggc
     2581 cagcagggcc tcggcgcggc ggcgctgcgc cctggcgtcg atgaggccga agcgccgcgg
     2641 cggcgacgtg atcgagatat tatccgcgac cgagaggtcg ggcatcagcg acagctcctg
     2701 gaagatgcac acaacgccag ccgcgttggc ggcggacggc gtggcgaagc tgacatcgct
     2761 tccggcaagg cgcatgccgc cggcatcggg ctgcaccacg ccggcgatga ttttgatcag
     2821 cgtcgacttg ccggcaccgt tctcgcccag caccgcatgg atcttcccgc gctcgcaggc
     2881 gaagtcgacg ccctcgaggg cgcgcacgcc accgtagcgc ttggagatgc cggacaatgt
     2941 gaggaaggtg ggttgcgtcg ggaccgcatc ctgcatggcg cgggctcagg cggcgacggc
     3001 ctggcggatg ccaggccgtg actggaacgt agccgcagcg gtcactgggt gttcttctcg
     3061 ctctgcgaca tgatctcggg cgctgtgaaa ttcacgccgc acgggaggaa ctggttcgga
     3121 gcgaagaagt tggcgctgag gtccggccag taattcgtgc cgggcttgag atccttgtag
     3181 gtcgcgaccg ggatcgggat cgagatcaac tgcggcatga cattgccctg cagcgccgag
     3241 atcgccgcct tggtggcgat ggcgactagc gccggcgact ggccgtagga catgcccttc
     3301 aggccgtcct tggcgtgatc ggcgatctgc ttgcggaact cgttctcgcc ctcacccgac
     3361 atcggcacga aagcatgctt ggcggcgacc atcgcctgta ccgtgccatc cgagccgcct
     3421 tgcgtgaaca cgccgtcgaa atggccgtgc acggcgagcg catcggcggt caccttctgc
     3481 gaggtgccgg tgtcccaatt gcccaccacc tcggtgatgt tgaacttgtt gccggcgcct
     3541 tccatgattt cgcggaaacc gatatggcgg tcacggtcga ccgaattgcc cggcaggccg
     3601 cgcacctcga ggatatcgcc ggccttcttg cccgattcat cgatcagcca tttggctgac
     3661 atgcggccca tctccttctg gtcctcgttg accatcatca ccttgtcggt gtcgaggatg
     3721 ttgtcgaagg gcaccaccac cacattgttc ttgtcggcga ggcggatgat acggtcgaag
     3781 ccgtcgggcg ccaccgcgat ggtgatgatg gcgtcgaagc cctgattgat aaaatcctcc
     3841 atggcgccga gttgcgccgc gacatcggtg ccggtcgaga ccaccttgaa ctccttgatg
     3901 ttctccttga tgcccgcctg ctcggcaaag gccttggcgg tcttcaccat ctggatgcgc
     3961 caggtattgc cgacgaagcc gttgacgagg gcgatcttgt aggggccggg cttcttgtcc
     4021 cactggaaga acttggtctg atccgaccag ggcttgaagc agccgggatt ggcacccggt
     4081 ccggcgacga tcttggggcc tgcccaagca ctgactgagg atagcaggag gctggcgcaa
     4141 gcaacccgcg taagaccaag cgtccacttc atggctcttc tccctgagcg ttgtgtttcc
     4201 gaccctatcg catgccggga attggacttc ccgtgcactt gccagaatgg cacgcaaggt
     4261 gcgatgcgca caagccgaat gaaaaaagca acggtagaaa tcaaatccaa aagcagcaaa
     4321 aatcgcgatg atagcgatgt cgacaggagc acgcgggggg taggcagtgt tttcggcgtg
     4381 caataaggac ccccgttttt cgggtgatcg gcatccaatc gagcaccccg accggggtcc
     4441 agcaaagtga attagaaagg cgggatttcg atgtggtatg gtcaagtcga gggaacggac
     4501 atcgtcgttc tcgatccgcg cttcaaggct tgcttcgccg gtcacgtgcg ggtcgagcgc
     4561 ttgtggtcgg gggcgcgctg ggctgaaggt ccggcctggt tcgcggcggg ccgctatctc
     4621 gtctggtcgg acattccgaa caatcgcatg atgcggctcg tcgaaccttc gggcgaagtc
     4681 tcggtctttc gcgagcgctc caacaattcg aacggcaaca ccgtcgacaa tcaaggccgg
     4741 ctggtcacat gcgagcatct cacgcgccgc gtgacccgca ctgagattga cggctcgatt
     4801 tccgtcattg tggaaaagtg gaacggcagg cgtctgaatt cgcccaacga cgtcgtcgtg
     4861 aaatcggatg actcgatctg gttcaccgac cccgcctacg gcatcgacac cgactacgaa
     4921 ggggatcgcg ccgggcagga gatcgagggc tgccatatct atcgtgtcga cgccaaatcc
     4981 ggcgcggtcg ccgctgtaat caccgatatg gttcgcccga acggcatcgc cttctcgccc
     5041 gacgagaaat acctctatgt cgccgacacc ggcgcgaccc acccccagtg gcccggttgc
     5101 tgatgggcaa agctgttggg gatgtagtcg gtacatccgg tcaagagctt gagattgttg
     5161 cgatctcgta gcacgcccac accggacgca gccgacgttc ggaatgcccg ctttgcgcaa
     5221 gaagcggtca tcgcgagaac gctggattcg atcctctagg agcccgtcca gataagtttt
     5281 gtgacggaca tttgttgcag ggtagccggc tcaagcggcg attgcgatgg tgaacagctt
     5341 caggaggttg tgggcggtgc agatcatcgc ccactcagcg cgcactttct cga
//
LOCUS       DATNOI010000492        12981 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_245822,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000492 DATNOI010000000
VERSION     DATNOI010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12981
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245822"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VN968_19720"
     CDS             complement(<1..621)
                     /locus_tag="VN968_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HXL31498.1"
                     /translation="MAEHAVEFGKDGRAVLRDGRLDAPAFHRNHQAIWTVLQQFLSGK
                     SGDVVEAGSGTGQHVVDFARHTPDITWWPSDLNEQHLKSIAAWRAHAGLPNVRPPLRI
                     DLTDPLWCPEMHDGTGPGELLAVFCANVIHIAPWRVAEGLFAGTGRYLRADGRLFLYG
                     PFKRDGKHTAVSNAVFDSSLREGNPEWGVRDIEALQELAAAAGLALV"
     gene            893..1234
                     /gene="soxX"
                     /locus_tag="VN968_19725"
     CDS             893..1234
                     /gene="soxX"
                     /locus_tag="VN968_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HXL31499.1"
                     /translation="MLQALALTLLIGAVAPLSPAKAQSAAAEGQKLAFDRGKGNCLTC
                     HEIKGGDFPGTIGPALKDIKSKYPDRNDLVAILFDETRRNPQTVMPPFGRNRILTEQE
                     INAVVDFLQTL"
     gene            1267..1761
                     /gene="soxY"
                     /locus_tag="VN968_19730"
     CDS             1267..1761
                     /gene="soxY"
                     /locus_tag="VN968_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HXL31500.1"
                     /translation="MTTPHDGFPATRRLILQGAATVALVGLGNVPFGLAAAFAAANDK
                     YPEDAFKAKSEADAIKSLYGKTAEPSDKVKLDAPEIAENGAVVPISVSTTLTEVTSIS
                     FLVSENPNALAASYKIPAGTMPSVANRLKMAKTSNVIAIVEAGGKLYSATKEVKVTVG
                     GCGG"
     gene            1786..2097
                     /gene="soxZ"
                     /locus_tag="VN968_19735"
     CDS             1786..2097
                     /gene="soxZ"
                     /locus_tag="VN968_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HXL31501.1"
                     /translation="MVSTIRVRATSSGETTEVQALIQHPMDSGFVKDTKGEIIPPHFI
                     RELTFEHDGKNVFVADWGAAVSKDPYVKFSFKGGKKGDDLKISWVDNKGATDTTTAKI
                     Q"
     gene            2166..3059
                     /gene="soxA"
                     /locus_tag="VN968_19740"
     CDS             2166..3059
                     /gene="soxA"
                     /locus_tag="VN968_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HXL31502.1"
                     /translation="MAGEVNVRPAIQFAAAVAVSAALVSAALAFGGVPSAVAADKPDP
                     VAEAKAFRDYFTKKFPQLKLEDFVNGPYSMNEDMHRQWEEKEQFPPYEFSLDMGKEIF
                     TKPFKNGKSYADCFPNQGIGVRQNYPTFDEKEGKVVTLELALNRCREANGEALFSYVK
                     DEMAALTAYMAFTSRGKPFDIKIPDDPRALEAYQNGKRYFYTRRGQLNFSCATCHVQE
                     PGERIRAEVLAPALGILNAMPIYRSEWSGMGTTSRRFTTCNSQIRGVPLDPQADEYRD
                     LEYYLSYVSNGLPISGPGARP"
     gene            3179..3490
                     /locus_tag="VN968_19745"
     CDS             3179..3490
                     /locus_tag="VN968_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31503.1"
                     /translation="MRALIVSLSSLFLIGGAASALAASSEADFKTAYAAAEAANKEAG
                     SLRNQWTVTAAALSAAKKAADAGDFDQAVAQSKEAEALARASIYQATSEKEAWKALEI
                     K"
     gene            3519..5267
                     /gene="soxB"
                     /locus_tag="VN968_19750"
     CDS             3519..5267
                     /gene="soxB"
                     /locus_tag="VN968_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HXL31504.1"
                     /translation="MTLRRRDFLKFTGAAALSGGLPLIARSADSAGVYDLERFGNARI
                     LHITDTHAQLQPAYFREPSVNLGVGGMQGKPPHLVGRAFLDRFGVRPDSADAYAFTSI
                     DFEKSAARFGKLGGFAHLKTLVDRLRSDVGPERSLLLDGGDSWQGTGQANILQGADMV
                     EAANLLGIEAMTGHWEFTYGEAALRSNLERFKGEFLAQNVYLTEEAAFNDAKAFDPAS
                     GRVFKPAMIKEIGGYRVAVIGQAFPYVPVAHPKRFTPDWTFGIRDDELQKLVSGLRSS
                     DKVDAVVLLSHNGMDVDLKLAGRVTGIDVILGGHTHDAVPQPIAVTNAGGTTLVTNAG
                     SNGKFLGVLDLEIAKGKVSDARYRLLPVFSELLKPDPAMQALIQKMREPQAADYAEKI
                     ATADRLLYRRGNFFGTVDQLICDALRSELNAEIALSPGFRWGSSMLPGQPVTMEDVLS
                     ETAITYPETYVQSLTGGQIKGILEDVCDNLFNADPYYQQGGDMVRVGGMAYTCTPAES
                     VGRRISELKLANGRALSAGKSYRVAGWASVNPQQGMPVWDVFAKYLRSGKAPDPRGAG
                     VILKGVDDNPGIAGQG"
     gene            5430..6470
                     /locus_tag="VN968_19755"
     CDS             5430..6470
                     /locus_tag="VN968_19755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL31505.1"
                     /translation="MIFRQLFDSVSGTYSYLLASRAGGEALILDPVLEKVDRYCQLLR
                     ELDLKLVKAVDTHLHADHVTGLGELRDRTHCVTIMGEQSKADVVAMRVADGDKVMIEG
                     LGLDVMYTPGHTDDSYSYLMGDRVFTGDTLLIRGTGRTDFQNGSARAQYDSIFGRLLK
                     LPDETMVFPAHDYKGDTVSTIGEERRFNPRLQVRSVDEYVELMGNLNLPNPKLMDVVV
                     PANMHVGLHQDDLAKQGLAFSAREGIESLGRTDILLVDLRENSERAKHGMLPGALHAP
                     YPSIGESLLPGGMLREVAAATGRRIVFFCAFGERSAMAVAAAKEAGLTNTAHIAGGMD
                     AWKKAGGPTLTG"
     gene            6755..6988
                     /locus_tag="VN968_19760"
     CDS             6755..6988
                     /locus_tag="VN968_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXL31506.1"
                     /translation="MDDEPKSAAELKEIRLNRKLAQDAEGIKAMADIAAADIAIRKRT
                     ENLRAIRLAKEAADRDKPAEPKAKAKLKKKKST"
     gene            6989..7270
                     /locus_tag="VN968_19765"
     CDS             6989..7270
                     /locus_tag="VN968_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31507.1"
                     /translation="MTAEDRNRADARFNKAARAANEAKTGKSDRDVAAKAVLDNMAKL
                     KALRLAREAAAPPPAATVKKTRAKPKKPGEKGPALADWLAGQQSGGRRT"
     gene            complement(7272..7514)
                     /locus_tag="VN968_19770"
     CDS             complement(7272..7514)
                     /locus_tag="VN968_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31508.1"
                     /translation="MKKPKLETEEQQPRKIIRADKAPTEGYSLVVDGHFKSHHDTVEA
                     AEEAGMALKNQYQMLQVQVYDAATKTRSMVEWPAPV"
     gene            7677..7832
                     /locus_tag="VN968_19775"
     CDS             7677..7832
                     /locus_tag="VN968_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31509.1"
                     /translation="MGETGKKTDRSKKDDSESKKSPPASIADDDIEDGDIATPKRDRH
                     GDDDEPL"
     gene            complement(7886..8257)
                     /locus_tag="VN968_19780"
     CDS             complement(7886..8257)
                     /locus_tag="VN968_19780"
                     /inference="COORDINATES: protein motif:HMM:NF015921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31510.1"
                     /translation="MPDTVLRADLGYWSSMFVAGSLGTVIGDYCSHDLHLGDGGASLL
                     LTPVLALLFVTARNGLLRSLPYYWLTVVAVRAAGTAVGDLVAGRNLLGLPLSTLVTGV
                     LFIALLAIWNEPMAPKLARAD"
     gene            8289..8879
                     /locus_tag="VN968_19785"
     CDS             8289..8879
                     /locus_tag="VN968_19785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31511.1"
                     /translation="MPTCKQRDSQNRDKSDDDRKPRKLHIAAGEVGEVCGPGPDDDDR
                     SPVIHLMDGPVEALADDNDRESEHQKWQSSDVPQSQIAREEIAHVCAKDAGEAERRPV
                     SRAKRWQVLLLHADRPLSVLNCKMVAGALHLAEAGTRMDMALRVRRGRRLERQSMKYE
                     NRLRKVPDVTLAFWVIKIAATTLGEPAGIPKQTQRI"
     gene            9001..10239
                     /locus_tag="VN968_19790"
     CDS             9001..10239
                     /locus_tag="VN968_19790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL31512.1"
                     /translation="MVDILAAPQQTKTDASLRTLAAISTAHWVSHFHLFVLPMLFPFL
                     KAQLGVGYIELGFALTVFAVTSGLTQAPIGYLADHIGARKVLLMGLTLGGFALIMLGL
                     HLSYPFLIASAVLLGLANSVYHPADYAILSAHMDEARMGRAFSIHTFAGFLGGAVAPA
                     IVAALVATTGGFGALIVAGAVGPLVALLLLIVGIPDASAADRHVDGALAPQQNIITPT
                     LMVLTIFFMLLSLSSAGIGNFGVVALMSGYGASFSSANIALTAFLGFSAAGVLAGGFL
                     ADRTRRHGQVAAACFAINAAIVLIIATATLPALLLTTAMATAGFLGGVIAPSRDMLVR
                     SAVPAGAAGRAFGIVSTGFNLGGIVSPLLFGWIMDQNMPHWVFGASVIFMILTVLLAL
                     FTDRGPQQRPAGADARLMHS"
     gene            10317..12059
                     /locus_tag="VN968_19795"
     CDS             10317..12059
                     /locus_tag="VN968_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL31513.1"
                     /translation="MADHPDLVIRGGSIADGLGGELFEADVAIANGRIVEVGRVQGKG
                     TEEIDARGKLVAPGFVDLHTHYDGQVTWSHDITPSSQNGVTTAVMGNCGVGFAPCRPS
                     DHQRLIQLMEGVEDIPEPVLSAGLPWAWESFPDYMDWLSQRNFDMDIGAQLPHAALRV
                     YVMGERGARRDPSTPEDNHAMAALAGDAVRAGALGFSTSRTLNHRTSTGDFTPTLKAG
                     EDELTAIAGAMHKVGRSVLQFVLDQSTIDEDLPMMLRVAENTRCPISFSVAQADKAPR
                     RWRQTLDRINEASGRGLSITAQIAARPVGLLLGLELSRNPFQTHPSYNAIARLPLAER
                     LQRLRQPEVRAAILSETATSTDDPLFFRPNYDKMYLLGDPPDYEQPPENALGAQARRQ
                     GRQPEELAYDAMLRDDGRGMLYVPFLNYADGNLDAVREMLRDPHSVPGLSDGGAHCGI
                     ICDASFPTYLLTHWTRDRTRGEKLSIPFVVAAQSRKTALSVGLYDRGVIAPGYKGDVN
                     VIDYDRLHLHPPKVHYDLPVGGRRLLQQVDGYDATIVSGVVTQRGGSATGARPGRLVR
                     GAQGSAPRIEAAAS"
BASE COUNT         2567 a         4038 c         4013 g         2363 t
ORIGIN      
        1 caccagcgcc agaccggcgg ccgccgccag ttcttgcagg gcctcgatat cgcgcacgcc
       61 ccattcggga ttgccttcgc gaagactcga atcgaacacg gcgttgctga ccgcggtgtg
      121 cttgccgtcg cgcttgaacg gtccgtagag gaacagccgc ccgtccgccc gcaagtaacg
      181 cccggtgcct gcgaacagac cctcggcgac gcgccacggc gcgatgtgga tcacattggc
      241 gcagaacacc gcgagcaatt cgccggggcc ggtgccgtcg tgcatctccg gacaccacag
      301 gggatcggtg aggtcgatcc gcagcggcgg gcggacattc ggcaaccccg catgcgcgcg
      361 ccaggcggca atgctcttca gatgctgttc gttgaggtcg ctcggccacc aggtgatatc
      421 gggggtgtgg cgggcgaaat cgactacatg ctggccggtg ccgctgccgg cctcgaccac
      481 gtcgccggat ttcccggaaa ggaattgctg caataccgtc cagatcgcct gatgattgcg
      541 gtgaaatgcc ggcgcgtcga gacggccgtc gcgcaagacc gcgcggccat ccttgccgaa
      601 ttccaccgcg tgctcagcca tgacgctctc ccggatttaa ttggcgggcc aagcttagcc
      661 agtgtaagcc gcctccgcgc gaaccgcgaa tccgactggc atttggcggg attctgggac
      721 cttgggcttt tttcgggtgt gcgagaagac attaacgctg cttccgcgat cccgccgtca
      781 gcgttttcaa ccgatggcgt ttatgcttgt gaccgcctat attctcaggg attgtcgaca
      841 taaaccccgc cggggagcgc ctttgaccaa cactttttcg aggaagcccg cgctgttgca
      901 ggcgctcgcg ttgacgttac tgatcggcgc ggtcgcgccc ttgagcccag ccaaggccca
      961 atccgccgcg gccgagggac aaaaactagc cttcgatcgc ggcaagggca attgcctgac
     1021 ctgccacgag ataaagggcg gcgattttcc cggtaccatc gggccggcgc tgaaggatat
     1081 caaaagcaaa tatcctgatc gtaacgatct cgtcgccatc ctcttcgatg aaaccaggcg
     1141 caacccgcaa accgtgatgc ctccgttcgg acgaaatcgc attttgaccg aacaggaaat
     1201 caatgcggtg gtggatttcc tccagacatt atgatggcga gcgcgcccct caggagacct
     1261 taccagatga ctacccctca cgacggcttt ccggccacgc ggcgcctgat cctgcaaggc
     1321 gcggctacgg tcgcgctggt cggcctcggc aatgttccgt tcggcctggc ggcggccttc
     1381 gccgcggcca acgacaaata tccggaagac gcgttcaaag cgaagagcga agcagacgcg
     1441 atcaagtcgc tctatggcaa gaccgcagag ccttccgaca aggtcaagct cgacgcgccg
     1501 gaaatcgccg agaacggcgc ggtggtcccg atttcggtca gtaccactct cactgaggtc
     1561 acctcgatct cgtttctggt gagcgaaaat ccgaacgcat tggcggcgtc ctataaaatt
     1621 ccggccggga ccatgccgag cgtggccaac cgtctgaaga tggcaaaaac cagcaatgtg
     1681 atcgcgatcg tggaagccgg cggcaagctc tacagcgcga ccaaggaagt caaagttacc
     1741 gtcggcggct gcggcggctg aggcggcatt caaggagata ttctgatggt atcgaccatt
     1801 cgcgtgcgcg caacttcgag cggcgagacc accgaagtcc aggccctaat ccagcatccg
     1861 atggattccg gctttgtcaa ggacaccaag ggcgagatca tcccgccgca tttcatccgg
     1921 gagctgacgt tcgaacatga cggcaaaaac gtattcgtcg cggattgggg tgctgcggtc
     1981 tcgaaggacc cctacgtcaa gttcagtttc aagggcggca agaaaggcga cgatttgaaa
     2041 atcagctggg tcgacaacaa gggcgcgacc gataccacca cggcgaagat acagtaacag
     2101 ctcggatttt ttgttttgac gcgttttctt cacgcgaacc ggtacccacc tcgcttgaaa
     2161 acgctatggc tggagaagtg aacgtgcgac ctgcgatcca atttgccgcg gccgttgctg
     2221 tctcggccgc tcttgtgtcg gcagcgcttg cgtttggcgg cgtgccgtcc gccgtcgccg
     2281 ccgataaacc cgaccctgtc gccgaggcca aggcattcag ggattatttc actaagaaat
     2341 ttccccagct aaagctcgag gattttgtca acggccccta ttcgatgaat gaggacatgc
     2401 accggcaatg ggaggagaaa gagcaattcc cgccgtatga gttctcgctc gacatgggca
     2461 aggagatatt cacaaaaccc ttcaagaacg gcaagagcta tgcggattgc tttcccaacc
     2521 agggcatcgg tgttcgccag aactacccca ccttcgacga gaaggaaggc aaggtcgtta
     2581 cgctcgagct ggcgttgaac cgttgccgcg aagccaacgg tgaggcgctg ttctcctatg
     2641 tgaaggacga gatggcggcg ctgaccgcct atatggcgtt cacctcgcgc ggcaagccgt
     2701 tcgacatcaa gatccccgac gatccgcgcg cgctggaagc ctaccagaac ggcaagcgct
     2761 atttctatac gcggcgcggt cagctcaatt tctcctgcgc gacctgccat gtgcaggaac
     2821 ccggcgaacg gattcgcgcc gaagtgctgg cgccggcgct cgggattttg aatgccatgc
     2881 cgatctaccg ttccgagtgg agcggcatgg gcaccaccag ccggcgcttc accacctgca
     2941 acagccagat ccgcggcgtg ccgctcgatc cgcaggccga tgaataccgc gatctcgaat
     3001 attatttgtc ctatgtcagc aacggactgc cgatttccgg tcctggggcg cggccatgag
     3061 caagatccgg aaaagcatgt tctcgggcta cgacccgatg ggtgggaacc ggttttccct
     3121 cgcgacaaac gcggaacacg tttgcgcgga gatcacgctc gaaagaaaaa gcaaagtcat
     3181 gcgggcattg atcgtatcgc tatcgtcgtt gttcctgata ggcggtgcag cttcggcgtt
     3241 ggccgccagc tcggaagctg atttcaagac cgcctacgcc gcggccgagg ccgccaacaa
     3301 ggaagcaggg agcttgcgca atcaatggac cgtgaccgcg gcggctctgt cggccgcgaa
     3361 aaaggcggcc gatgccggcg acttcgacca ggccgtggcg cagtcaaagg aagccgaagc
     3421 gctggccaga gcttcgatct accaggcgac cagcgaaaaa gaagcctgga aagcacttga
     3481 gataaaatga gcttttgcaa aaggcgcgga gacatcgcat gaccttgcgc cgccgggatt
     3541 ttctgaaatt cacgggtgct gccgcgcttt cgggcggatt gccgctgata gcgcgcagcg
     3601 ccgacagcgc aggcgtttac gatctcgaac gcttcggcaa tgcgcgcatc ctgcacatca
     3661 ccgacacgca tgcgcagctg cagccggcct actttcgcga accgagcgtc aatctcgggg
     3721 tcggcggaat gcagggaaag ccgcctcatc tggtgggccg cgcctttctc gaccggtttg
     3781 gcgtaaggcc cgacagcgcc gacgcctacg ccttcacatc catcgatttc gagaaatccg
     3841 ccgcgcgctt cggcaagctc ggcggctttg cgcatctgaa gacgctggtc gaccggctgc
     3901 gcagcgatgt aggacccgaa cgctcgctgc tgctcgatgg tggcgattcg tggcagggca
     3961 ccggacaggc caacatcctg cagggcgccg acatggtcga ggccgcgaac ctgctgggca
     4021 tcgaggcgat gaccggccat tgggaattca cctatggcga ggcggcgctc cgcagcaacc
     4081 tcgagcgctt caagggcgaa ttcctggcgc agaacgtcta tctcaccgag gaagccgcat
     4141 tcaatgacgc caaggcattc gatccggcgt cgggacgcgt gttcaagccc gccatgatca
     4201 aggagatcgg cggctatcgc gtcgccgtga tcgggcaggc tttcccctat gtgccggtcg
     4261 cccatccaaa acgctttacg ccggactgga cgttcgggat ccgcgacgac gaattgcaga
     4321 aattggtgag cggcttgcga tccagcgaca aggtcgatgc ggtggtgctg ttgtcgcata
     4381 acggcatgga cgtcgacctc aagctcgccg gccgcgtcac ggggatcgac gtcatcctcg
     4441 gcggccatac ccacgacgcc gttccgcagc cgattgccgt caccaatgcc ggcgggacca
     4501 cgctcgtcac caacgccggc tcgaacggaa aattcctggg cgtgctcgac ctcgaaatcg
     4561 ccaagggcaa ggtcagcgac gcgcgctacc ggctgcttcc ggtgttctcg gaattgctga
     4621 aaccggatcc ggcgatgcag gcgctgatcc agaaaatgcg cgagccgcag gccgccgact
     4681 acgccgagaa gatcgccacc gccgatcgcc tgctctatcg ccgcggcaat ttctttggca
     4741 ctgtggatca attaatctgc gacgcgttgc gaagcgaact caacgccgag atcgcgttgt
     4801 cgccgggctt tcgctggggc tcaagcatgc tgccgggcca gcccgtgacg atggaagacg
     4861 tgctgtccga gaccgccatc acctatccgg aaacctacgt ccagagcctg accggcggcc
     4921 agatcaaggg tatcctcgaa gacgtctgcg acaatctctt caatgccgat ccctactatc
     4981 agcagggcgg cgatatggtt cgcgtcggcg gcatggccta cacctgtacc cccgccgaaa
     5041 gcgtcgggcg gcggatttcc gagctgaagc tcgctaacgg ccgcgcgctt tcggccggca
     5101 agagctacag ggtggccggg tgggcctccg tcaacccgca acagggaatg ccggtgtggg
     5161 acgtttttgc caaatatctg cgttccggga aggcgccgga tccacgtggc gcgggtgtca
     5221 tcttaaaggg tgttgacgat aacccgggca tcgcaggaca aggatgaggc gtttctcggg
     5281 acatccggca agcggccgga gtttcatttc cgtcgcgacg ccggtccagg tcggcgtcgg
     5341 gcagattctg gcgctgaccg aaaacggata tacattagtt cagccgtaga attttcaagg
     5401 taatatcggg aatcaatttg gaggcgacca tgatttttcg ccaattgttc gacagcgttt
     5461 cgggaaccta cagctatctc ctggccagcc gtgccggcgg cgaggcgttg atcctcgatc
     5521 ccgtgctgga gaaggtcgat cgctactgcc agttgctgcg cgaactcgat ctcaaacttg
     5581 taaaggccgt cgatacccac ctgcacgccg accacgtcac cgggctcggc gagctgcgcg
     5641 accgcaccca ctgtgtcacc atcatgggcg agcagagcaa ggccgacgtg gtggcgatgc
     5701 gggtcgccga cggcgacaag gtgatgatcg agggcctcgg tctcgacgtg atgtacacgc
     5761 ccggtcatac cgatgattcc tacagctatc tgatgggcga tcgggtattc accggcgata
     5821 cgcttctgat ccgtggcaca ggccgcaccg atttccagaa cggcagcgcg cgagcgcaat
     5881 acgattccat cttcggccgg ctgctgaaac tgcccgacga gaccatggtt tttccggccc
     5941 atgactacaa gggcgatacc gtctcgacca tcggcgagga aaggcgcttc aatccgcggc
     6001 tgcaggtgcg ctcggtggat gaatatgtcg agctgatggg caatctgaat ttgcccaacc
     6061 cgaagctgat ggatgtcgtg gtgccggcca acatgcatgt cggcctgcat caggacgatc
     6121 tggcgaaaca aggtctggcg ttcagcgccc gggagggaat cgagagcctc ggccggaccg
     6181 atattctgct ggtcgatctc agggaaaaca gcgagcgcgc caagcacggc atgctgccgg
     6241 gcgcgctgca tgcgccctat ccttcgatcg gcgagagcct attgcccggc ggcatgttgc
     6301 gcgaggtggc cgcggcgacc gggcgccgca tcgtgttctt ctgcgcgttc ggcgagcgct
     6361 ccgccatggc cgtcgccgcc gccaaggaag ctggcctcac caacaccgcg catatcgccg
     6421 gcggcatgga tgcgtggaag aaagctggcg gaccgacact gacggggtga ggtaagccgc
     6481 gtcgtatccg acggctcgtt cagcgcctat ccgccgccgg caagaacatg gtaaagcccg
     6541 gagcgacgat cggcctgtgc gaagcctgcg agagcggctt gagcccggtc gcgcgcgaac
     6601 atcccttcac cacgattgcc gatttccggt caaaaggggc gctttaccga cgattccggc
     6661 ccaaagatgc ccctttggcg gtttaaatgc gtggtaggat cgaaccagag gtcgccgccc
     6721 gatcgtgcgc gccgcgtgaa ttttgaggta tccgatggat gacgaaccga agagcgccgc
     6781 cgaactgaag gagatccggc tgaaccgcaa actcgcccag gatgccgaag gcatcaaggc
     6841 gatggcggat atcgcggcgg ccgatatcgc gatacgaaag cggaccgaga acctgcgcgc
     6901 gatccggctg gcgaaggaag ccgccgaccg cgacaagccg gcggaaccca aagccaaagc
     6961 taaactcaaa aagaagaagt cgacttaaat gacagcagaa gatcgcaacc gcgccgacgc
     7021 gcgcttcaac aaggctgcac gcgccgcgaa cgaagccaag accggcaaga gcgaccgcga
     7081 tgtcgccgcc aaagccgtgc tcgataacat ggcgaagctg aaggcattgc ggctcgcccg
     7141 cgaggcagcc gcccctccgc ctgccgcaac cgtcaaaaag acgcgcgcca agccgaagaa
     7201 gccaggagaa aagggccccg cattggcaga ttggctggcg ggacagcaaa gcggtgggcg
     7261 gcggacttag tctagacggg cgccggccat tcgaccatcg atcgggtctt ggtcgcggca
     7321 tcgtagacct gcacctgaag catctggtat tgattcttca acgccatgcc cgcctcttcg
     7381 gcggcctcga ccgtatcatg gtgcgatttg aagtggccgt ccaccaccag cgagtagcct
     7441 tcggtcggcg ccttgtcggc gcgaatgatc ttgcgcggct gctgctcttc tgtctctagc
     7501 ttcggctttt tcatgtatgg ctccgtgttg cgcctccctt tacactgaac gtggtaaaaa
     7561 ggcaggaaag attatccggc aacggctaat atgtcggtca aaggcagtgg gattgagatc
     7621 gaacgctgtg gtaatcaagt ggtttgcggt cgattcatcc gattgggggt aacagcatgg
     7681 gtgaaacggg caagaagacc gatcgttcca agaaggacga cagcgaatca aaaaaatcgc
     7741 cgccggcgtc gatcgccgac gacgacatcg aggatggcga catcgccaca ccgaaacgtg
     7801 atcgtcacgg cgacgacgat gagccgctat gactttggca gggtcatgag ctcatcggcg
     7861 cagccatgcc atgaccgcgc gatcgctagt ccgcgcgcgc cagtttcggt gccatcggtt
     7921 cattccatat cgcaagcagc gcaatgaaca gcacgcccgt gacgagcgta ctgagcggaa
     7981 gacccagcag attccggcct gcgaccaaat ctccgacggc ggtgccggcc gccctgaccg
     8041 ccacgaccgt caaccagtaa tacggcagcg atcgaagcaa tccgttacgg gctgttacaa
     8101 acagcaacgc caggactgga gtgagcagca acgacgctcc gccatcccca agatgcagat
     8161 cgtgcgagca gtaatcgccg atcaccgtac ccaggctccc ggcaacgaac atgctgctcc
     8221 aatagccgag atcggcgcga agcacggtat ccggcattac ttttgtcgga ctgccggcgc
     8281 cagacgaaat gccaacttgc aaacagcgcg acagccagaa tcgcgacaag tccgacgatg
     8341 accgaaagcc tcggaagctt catatcgccg caggcgaagt cggcgaagtt tgtggccccg
     8401 gtcctgacga tgatgatcgc agtccagtaa tacacctgat ggacggaccg gtcgaagcgc
     8461 tcgccgatga caacgaccgc gagagcgagc accagaaatg gcaatccagc gacgtgcccc
     8521 aatcccagat cgcgcgcgaa gaaatcgccc atgtttgcgc caaagatgct ggcgaggcag
     8581 agcgccgccc agtatcgcgc gccaagcgtt ggcaagtgct tttgttgcat gcagaccgtc
     8641 cactttctgt gttgaattgc aagatggtgg cgggtgcgct tcaccttgca gaggctggaa
     8701 cccggatgga tatggctctc cgagtgcgtc ggggccgacg cttggaacgg caatctatga
     8761 aatatgaaaa tcgcctgcgc aaggttccgg acgtcaccct ggccttttgg gtgatcaaga
     8821 tcgcagcgac cacgttggga gagccggcag gtatcccgaa acaaacccag aggatttaac
     8881 gattgctgtg cggctgccga gggctgccat gcaagggcat tcatggacaa ataaccgcct
     8941 ttggatagtt tctgacgggc ctcgccgggc cattacgatg agacggggtt tggattagag
     9001 atggtcgaca ttctcgccgc accgcagcaa acgaaaacgg acgcatcgct gcgcacgctg
     9061 gccgcgattt cgacggcgca ttgggtcagc cattttcatc tgttcgtgct gccgatgctg
     9121 ttcccgtttc tcaaagcgca actcggcgtc ggctacatcg agcttggctt tgccctcacc
     9181 gtcttcgcgg tcacatccgg cctgacccag gccccgatcg gctatctcgc cgatcatatc
     9241 ggcgcccgca aggtgctgct gatgggcctg accttgggcg gcttcgcgct gatcatgctc
     9301 ggcctgcatc tgagctatcc gttcctgatc gccagcgccg tgctgctggg gctcgccaac
     9361 agcgtctatc atccggccga ttatgcgatc ctttcggccc acatggatga ggcgcgcatg
     9421 ggccgcgcct tttcgatcca taccttcgcc ggcttcctgg gcggcgcggt cgcgcccgcc
     9481 atcgtggcag ccctggttgc aacgaccgga ggcttcggcg ccctgatcgt ggccggtgcg
     9541 gtgggtccgc tggtggcatt gctgctcctg atcgtcggca ttcccgatgc cagcgccgcc
     9601 gatcgccacg tcgatggggc gctggcaccg cagcaaaaca tcatcacccc caccctgatg
     9661 gtgctgacga tctttttcat gctgctcagt ctttccagtg ccggcatcgg caatttcggc
     9721 gtggtggcgc tgatgagcgg ctatggcgcg tcgttctcgt cggccaatat cgcgctgacc
     9781 gcgtttctcg gctttagcgc cgccggtgtg ctggcgggcg gctttctcgc cgaccgcacc
     9841 cggcgccacg ggcaggtcgc cgccgcctgc tttgcgatca acgcggccat tgtgctgatc
     9901 atcgcgacgg cgacgttgcc tgcgctgctg ttgacgacgg cgatggcaac ggccggattc
     9961 ctcggcggcg tgatcgcacc ctcgcgcgat atgctggtcc gcagcgccgt gcccgcgggc
    10021 gcggccggcc gggccttcgg catcgtctcg accggtttca acctcggcgg catcgtcagc
    10081 ccgctcttgt ttggctggat catggaccag aatatgccgc attgggtgtt cggcgcgtcc
    10141 gtcatattca tgattctcac ggtcttgctc gcgcttttca ccgaccgcgg cccgcaacag
    10201 cgccccgcag gggctgacgc aaggcttatg cattcctgag cctcatcccg ggcggttgac
    10261 agcgacgcgt ggcaaccgat gatgcggcaa taaaattcaa agcgggaaga aacaccatgg
    10321 ccgatcatcc agacctcgtg atccgcggcg gcagcatcgc cgacgggctt ggcggcgaac
    10381 tgttcgaagc cgatgtcgca atcgcgaacg gccgcattgt cgaagtcggc cgggttcaag
    10441 gtaaaggcac cgaggagatc gacgcccggg gcaaactggt ggcgccggga tttgtcgacc
    10501 tgcacaccca ttatgacggc caggtcacct ggagccacga catcacgccc tcgtcgcaga
    10561 atggcgtcac caccgccgtg atgggcaatt gtggcgtcgg gttcgcgccc tgccgtccgt
    10621 ccgaccatca acggctgatt caattgatgg aaggcgtcga ggatattccc gaaccggtac
    10681 tgagcgccgg cctgccctgg gcctgggaaa gttttccgga ctacatggac tggctgtcgc
    10741 agcgcaactt cgacatggac atcggcgccc aactgccgca cgccgcactg cgcgtctacg
    10801 tcatgggcga acgcggcgcg cgccgcgatc cctcgacacc ggaggataac cacgcgatgg
    10861 cggcgctcgc cggcgatgcc gtgcgggccg gcgcgctggg gttctcgacc tcgcgcaccc
    10921 tcaaccaccg cacctcgacc ggcgacttca cgccgacgct gaaggccggc gaggatgaac
    10981 tgacggcgat cgccggcgcc atgcacaagg tcggccgcag cgtgctgcaa ttcgtgctcg
    11041 atcagagcac catcgacgag gacctgccga tgatgctgcg ggtggcggaa aacacccgct
    11101 gcccgatttc gttttcggtc gcgcaggccg acaaggcgcc gcgccgctgg cgccagacgc
    11161 tcgatcgcat caacgaggct tcggggcgcg gcctgtcgat cacggcgcag atcgccgcgc
    11221 gcccggtggg gctgttgctg gggctggaat tgtcgcgcaa cccgttccag acccatccaa
    11281 gctacaacgc gatcgccagg ctgccgctgg ccgagcggtt gcagcggctg cgtcagcccg
    11341 aggttcgcgc ggcgatcctg agcgagacag cgacctcgac cgacgatccg ctgttcttcc
    11401 gtccgaacta cgacaagatg tatctgctcg gcgatccccc ggattacgaa cagccgccgg
    11461 aaaatgcgct gggcgcgcag gcccgccgcc aggggcggca gccggaagaa ttggcctatg
    11521 acgcgatgct aagggatgac ggccgcggca tgctgtatgt gccgttcctc aactacgccg
    11581 acggcaatct cgatgccgtg cgcgaaatgc tgcgcgatcc gcattcggtg ccgggactga
    11641 gcgacggcgg cgcccattgc ggcatcatct gcgatgcgag cttcccgacc tatctgctga
    11701 cgcactggac ccgcgaccgc acccgcggcg aaaagctgtc gatccccttc gtggtggcgg
    11761 cgcagtcgcg caagaccgca ttgtcggtcg ggctgtacga tcgcggcgtg atcgcgcccg
    11821 gctacaaggg cgatgtcaac gtcatcgact acgacaggct gcatctgcat ccgccaaaag
    11881 ttcattacga tcttccggtg ggcggccgcc gcttgctgca acaggtcgat ggctatgacg
    11941 ccaccatcgt ctccggcgtg gtgacgcaac ggggtggctc ggccaccggc gcgcgtcccg
    12001 gccggctggt gcgcggcgcg cagggcagcg caccgaggat tgaagccgcg gcttcctagc
    12061 gcatggtcca gctgaattga agctggctcg atggcccggc aatgacggtg ccctttctcc
    12121 tctggggccc cacaaaggga gagagcgcgc gccgttgccg cgacctccaa tcggagcttt
    12181 atccggatgg atcagcctcg gtacaaattt ccgattcaat tttcaaacag cagccgtcaa
    12241 ctcactccgt cattgccggg catagctgtc cgaaggacgg cgtcgcttcc gctcgcctat
    12301 gccggcaatc catcttcttc gaaaatgatc atgcgaagat tgatggatgc ccggatcaag
    12361 ccctggcatg acgagtgtgt ttgcgtcgac atctcgattc caaacatgcg ttcgcgccct
    12421 cgcggcgcga tgcgcccgag gcgttgatga atcgtccgcc caaggagggc gtggggaatg
    12481 ccgggtgccc gctgcacccg cggcctcgtg cgcgctttgt agtggtagaa gcgcacgagt
    12541 aacgacgagt acaccggaat cgcccggcat tcccgcacgc aatggtttta acggcgtatg
    12601 tcgtgctctt cccggtgacc gggctctttt gccacgtcgt cctgcgaata tggaatgtcc
    12661 gcgcccggtt gggccgacaa tcctccgcaa gacttgacgc cagcgtcggg gcgtcagaac
    12721 cacacgattt ggccgtccgc tggcagcatc tctcgtccgc gtgcttaagg cgtcgctcac
    12781 ggattcaaag aacccgccct gcgatccctc gcgcgcaaga cgctgccgcg tccaccgcat
    12841 cccgcaccca acgtccgtga cgatcgcgat acgcccctct ctcgggccgg gatggcgagc
    12901 gtgataaaga tgatttgggg ggaatgggaa gcggaatatt tttgcggagg gggactggac
    12961 aggtagaaac agcttgatcc g
//
LOCUS       DATNOI010000493        10455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_728684,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000493 DATNOI010000000
VERSION     DATNOI010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728684"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="VN968_19800"
     CDS             <1..1421
                     /locus_tag="VN968_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXL31514.1"
                     /translation="LERGANRRARAFAARGVKPGDFVAIGLPNSNAFFETTFAVWKCG
                     ATPTSLSWRLPRGEAAAVLEILKPALVVGGEADWNAPNSLPADFVPEGFSDEPLNNPV
                     ARYWKAMTSGGSTGRPKVILDHGPAVVETAAEQMLGIPPGVSLLNPGPLYHNAPFILS
                     HMGLFAGGRVTGMVKFDAEEALRLIERNRVQWVNFVPTMMHRIWALPEEVRNAHDLSS
                     LQIVFHMAAPMPPWLKEKWIAWFGPEKIYELYGGTERQGATVISGVEWLAHRGSVGRI
                     GETSRLRIIGEDGNDVAPGETGEIYFLPNDGAGSTYHYLGAEPKRRPDGWESLGDIGR
                     LDADGYLYLGDRLADMILRGGANIYPAEVEAAVMAHPHVRSCVVVGLPDAEFGQRVHA
                     ILELAGDAEAQSVIDGMGGFLADQLSRYKHPESFEIVAVGPRDDSGKVRRTLLRDERA
                     AWLREGRAFRIMPSRGPAKRVD"
     gene            1623..2594
                     /locus_tag="VN968_19805"
     CDS             1623..2594
                     /locus_tag="VN968_19805"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HXL31515.1"
                     /translation="MPSLTEWKVPLASQPRAGDYSFDLDRVLASVVGLHSIIPADAFS
                     ADTLGTERAGNGVLIDDGLVLTIGYLITEAEAVWLHLGDGRVVEGHALGFDSVSGFGL
                     VQALGRIDLDPLPLGSSADAKVGDRVVVGGAGGRTRSVASHIAAKQEYAGYWEYLLDE
                     AIFTYPAHPNWGGTALISNRGELIGIGSLQLEREREGKAEHVNMIVPIDLLKPVIDDL
                     RKFGRVNKPARPWLGMFSTEIEDKLVVVGISAKGPAARAELKAGDVILAVKGEKISSQ
                     TQFYRNLWSLGPAGVDVPLTIYHEGVTFDVVLTSTDRGRLLKAPRLH"
     gene            2720..3805
                     /locus_tag="VN968_19810"
     CDS             2720..3805
                     /locus_tag="VN968_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HXL31516.1"
                     /translation="MSEAVVDDIVAVLPPLLQSLEALGFIERHLNPPAFDQVMKAAGT
                     PDQALQAVRPRLADWPEEFAHLRTPLASASDAAITAFEGLRAVQHGDGDLIAVFRALR
                     LAPRALEALYPLAAKLPPVSEFFVDPALREDAGLLSRLAEPARDNTGIVHDHNEPGSR
                     GGFSLYVPEYYTPDRAWPLVMALHGGSGNGRGFLWSWLRDARSHGAILVAPTATGNTS
                     NKTTWALMGEDTDSPNLARILDAVRGRWNVDPARLLLTGMSDGGTFCYVTGLEGASPF
                     THLAPVAATFHPLMAEMADARRLRGLPIHIVHGRLDWMFPVQVARKAREALTAAGADV
                     TYRELDDLSHAYPREMNAPILNWLRAE"
     gene            complement(3815..4555)
                     /locus_tag="VN968_19815"
     CDS             complement(3815..4555)
                     /locus_tag="VN968_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007703179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL31517.1"
                     /translation="MTDKTSKVAIVTGASRGIGAAVAERLAADGFMVVINYSGDTKSA
                     ETLARKVEAKGGRALTARADVSEPQAVRGMFDTTEAAFGGVDVLVNNAGIMKLARIAD
                     SDDALFDQQIAVNLKGSFNAMREAARRLRDGGRIVNFSTSVVGTRLETYAVYAATKSA
                     VETMTAILSKELRGRNITVNAVAPGPTATDLFLNGKSPELVDRFAKMNPLERLGTPED
                     IASAVAFLVGPDGGWINGQVLRANGGMV"
     gene            4758..6104
                     /locus_tag="VN968_19820"
     CDS             4758..6104
                     /locus_tag="VN968_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31518.1"
                     /translation="MPPNTAFASQPRPSRSSPSQLLSNGVTLLEGTLKLTTAILALAA
                     GVYTYLGVRELLNGSPTTVFFAAVIYSVAVSVGIYAFWVFLMQFMPHVLDRAGRGLMF
                     GCMLLGSLMIVAMSSWLNASALAGAAAIQQHLAITVQNYTRDLDTANSNAIAAQGLLP
                     DIQLASSRFAKLAEAERAGSLTGTSGSGTVVQLLTQMSGQLDSLGQEVQASGKRVSAL
                     YEQGSKNLAKMRELISDRGPISTRSDAFAAESLALMGVIANLQQTSVAPAVKRAATSL
                     SSGFIAPAAGGRTADLADRQTAVVGKVESSIATQAASLSDAADKILATPRVEPIRFQS
                     MSPAEAVLRYAGDFIPSWAGAISIDLMPAVLVLILCIVHAAIRREEIPAFSASNMTAG
                     ELLAALQMAREVEDARRGIPNEMVAATAKAEGEPAAAPAPGTEENVTSLSSARLTK"
     gene            6114..6974
                     /locus_tag="VN968_19825"
     CDS             6114..6974
                     /locus_tag="VN968_19825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31519.1"
                     /translation="MPQSSSMQQRFFVWLSNNPGEAILRWIFRVVVTVTIAALAVDLA
                     GMNGWGWGARPDPAATPAETSDLPSIVPSILAPLLPGGDKRLLPLPPPDGPMAKPMTF
                     ELVSGGKLMATGTITPGISEAFAAEVSRHGEYIKTVVLSSPGGSVADALTMGRLIREK
                     KFATEVEAGKFCASSCPLVFAAGVERRAGDRAAIGVHQVAAISSAANGPPRDEMKVAQ
                     NISARCQRYLADMGVNLQVWVHAMETPPDKLFVFKPDELKSLNMVTATPASTPAAPPV
                     TTSAGTKTRL"
     gene            7060..7266
                     /locus_tag="VN968_19830"
     CDS             7060..7266
                     /locus_tag="VN968_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31520.1"
                     /translation="MQTFIGMILGMLLLVAGVYIYDSMATSSVANGQVAQPNRTIVNW
                     DVAEVNWKALKARAHDDWIKLSSK"
     gene            complement(7363..8037)
                     /locus_tag="VN968_19835"
     CDS             complement(7363..8037)
                     /locus_tag="VN968_19835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HXL31521.1"
                     /translation="MNKSIDRAPPVRRRTRREALGLLGAGTLMLGVAPGPALGQGDDE
                     TVLTEALVLHDPDIPAAGNIDGDITIVEYFDYQCPYCRKLEPELRQVVQDDGKVRLVL
                     KDWPILGPVSVIAARMALASKFQDKYIQAHEALIGVNSKLTEPRMRELLAGAGIDVDR
                     LDRDLVTNTKAIDAIIARNNDQATAFGFKGTPSFIVGKFRVPGTLTMAEFDRVIADAR
                     KAKANK"
     gene            complement(8071..8850)
                     /locus_tag="VN968_19840"
     CDS             complement(8071..8850)
                     /locus_tag="VN968_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXL31522.1"
                     /translation="MISAMQGALGLASGALVGFSLGLVGGGGSILAVPLMVYVVGVPD
                     AHVAIGTSAIAVAANAAVNLSNHARGGTVMWSCALPFAVAGITGAFAGSVFGKMVDGQ
                     RLLALFGLLMLVIAMLMLKTRSRAGLPDVQMNWANMPAIVGLGLATGTLSGFFGIGGG
                     FLIVPALMLATGMPIMNAVSSSLVAVTAFGLTTAASYAWSGLISWGLAGLFIAGGVAG
                     GVIGTRFARHLSVRRGALNIVFAAVIIAVALYMLARNVPLS"
     gene            complement(8939..9661)
                     /locus_tag="VN968_19845"
     CDS             complement(8939..9661)
                     /locus_tag="VN968_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HXL31523.1"
                     /translation="MSENPFSLWVYLSQSPLLWLTVTLLVYATADAASLATRRHPLAN
                     PVLHSIWIIAAFLVLTGTSYTTYFGGAQFVHFLLGPATVALAVPLYENRKTVAAAILP
                     MLAALVAGSVTAIVSVVLLAEAAGLPRGLVLSLAPKSVTAGIAMGISESLHADPSLTA
                     VAVILTGIMGAIIVTPLMNRIGITDFRARGFAVGIAAHGIGTARAFQVDAVAGVFASV
                     AMSLNALVTSLLVPLAATLLVR"
     gene            complement(9658..10044)
                     /locus_tag="VN968_19850"
     CDS             complement(9658..10044)
                     /locus_tag="VN968_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HXL31524.1"
                     /translation="MIASLSLILLCQLAGEAIVRGLSLPMPGPVVGLLLLLLLLLARD
                     RFGVLARGPLQQDGVENASRGLLAHLSLLFVPAGVGVMQKLDVVADHGIAIAAVLAIS
                     VALTLLVTVATFLVASRLLARGRSAR"
BASE COUNT         1885 a         3438 c         3316 g         1816 t
ORIGIN      
        1 agctcgagcg cggcgccaac cgccgcgccc gcgcctttgc cgcaagaggc gtgaaaccgg
       61 gtgatttcgt cgccatcggc ctgcccaaca gcaatgcttt tttcgaaacc acctttgcgg
      121 tgtggaagtg tggcgcgacg ccgacctcgc tgtcctggcg gctgccgcgc ggcgaagctg
      181 ccgcggtgct cgagattctc aaacccgcct tggtggtcgg cggcgaagcc gactggaacg
      241 cgcccaattc gctgcccgcc gatttcgtgc cggaaggttt ttccgacgaa ccgctgaaca
      301 accccgtggc gcgctactgg aaggcgatga cgtccggcgg ctcgaccgga cggcccaagg
      361 taatcctcga tcacgggccc gcggtggtcg agacggcggc ggaacagatg ctcggcatcc
      421 cacccggcgt gtcgctgctc aatcccggcc cgctctatca caatgcgccg ttcatcctgt
      481 cgcacatggg attgttcgcg ggcgggcgcg tcaccgggat ggtgaaattc gacgccgagg
      541 aggcgctgcg cctgatcgag cgcaatcggg tgcaatgggt caacttcgtg ccgaccatga
      601 tgcatcggat ctgggcgctg ccggaagagg tgcgtaacgc ccatgatttg tcgagcctgc
      661 aaatcgtgtt tcacatggcg gcgccgatgc cgccatggct gaaggagaaa tggatcgcgt
      721 ggttcgggcc ggagaaaatc tacgaactct atggcggcac cgagcgccag ggcgccaccg
      781 tgatctccgg cgtcgaatgg ctcgcgcaca ggggctcggt cggcaggatc ggcgaaacca
      841 gccgcctaag gattatcggc gaggacggca atgacgtcgc gccaggcgag accggtgaaa
      901 tctatttcct ccctaacgac ggcgccggca gtacctatca ctatctcggc gccgagccaa
      961 aacgccgtcc cgacggctgg gaatcgcttg gcgacatcgg ccggctcgac gccgacggct
     1021 atctctatct cggcgatcgc cttgccgaca tgatcctgcg cggcggcgcc aatatctacc
     1081 cggccgaggt cgaggccgcg gtgatggcgc atccgcacgt gcgctcctgc gtcgtcgtcg
     1141 gcctgcccga tgccgaattc ggacaacgcg tccacgccat cctcgaactt gcaggcgacg
     1201 ccgaggcgca atcggtgatc gacggcatgg gcggcttcct ggccgaccag ctcagccgct
     1261 acaagcatcc ggaaagtttc gagatcgtcg ccgtcggccc gcgcgacgat tccggaaaag
     1321 ttcgccgcac cctgctgcgc gacgagcgcg cggcgtggct cagggaaggc cgcgcatttc
     1381 gaatcatgcc gtcgcgtggt ccggcgaaac gggttgatta gtcaggaacc tcgcacgata
     1441 attggcgcga tatccctccc ggagataccg catgcgattt cgcgggattc ttccgcaatc
     1501 ttcggtttca gggctggaag taacgcgccg gatatcccag attgttttcg atattacgtc
     1561 tcgacgccag ttgtgatcca atcgggctga atgctgatac ctatcgaacc ggaggattct
     1621 ccatgccctc tttgacagaa tggaaagtac cgctggcatc gcagccccgc gccggcgact
     1681 acagtttcga tctcgatcgc gtgcttgcct cggtggtcgg gctgcattcg atcattccgg
     1741 ccgacgcctt tagcgcggat acgctgggta ccgagcgcgc cggcaacggc gtcttgatcg
     1801 acgatggcct ggtactgacg atcggctatc tgatcaccga ggccgaagcg gtctggctgc
     1861 atctcggcga cggccgcgtg gtggaaggcc acgcgctcgg cttcgattcg gtcagcggct
     1921 ttggcctcgt gcaggcgctt ggccggatcg atctcgatcc gctgccgctt ggctcttccg
     1981 ccgatgccaa agtcggcgac cgcgtggtgg tcggcggcgc cggcggacgc acgcgctcgg
     2041 tcgccagcca catcgccgcc aagcaggagt acgccggtta ctgggagtat ctgctggacg
     2101 aggcgatctt cacctatccg gcgcatccca attggggcgg caccgcgttg atctcgaacc
     2161 gcggcgaatt gatcggcatc ggttcgctgc agctcgagcg cgagcgcgag ggcaaggccg
     2221 aacacgtcaa catgatcgtg ccgatcgatc tgctcaagcc ggtgatcgac gatttgcgca
     2281 aattcggtcg tgtcaacaag ccggcgcggc cctggctcgg gatgttctcg accgagatcg
     2341 aggacaagct ggtggtggtc ggcatctccg caaaggggcc ggcggcgcgc gccgagctca
     2401 aggccggcga tgtcatcctg gccgtcaagg gcgagaagat ctccagccaa acgcagtttt
     2461 atcgaaacct ctggtcgctc ggacccgcgg gtgtcgatgt gccgctcacg atctaccacg
     2521 agggcgtcac cttcgacgtg gtgttgacct cgaccgaccg gggaaggttg ctcaaggcgc
     2581 cgcggctgca ttgaacgcca gagcaggatc cgcaaaggca tgccctcggg ctgcgacccg
     2641 atcggcggca accggttttc cctcgcgaca aacgcgaagc gtttgtgcgg aaatcctgct
     2701 ctaataacgg aggggcgcga tgagcgaggc cgtggtggac gacatcgtgg ccgtgttgcc
     2761 gcccttgctg caatcgctcg aagcgctcgg cttcatcgag cgccacctga acccgccggc
     2821 gttcgaccag gtcatgaaag ccgccggcac gccggatcag gcgttgcagg cggtccgtcc
     2881 gcggctggct gattggccgg aagaatttgc acatttgcgc acgccgcttg cgagcgcaag
     2941 cgatgcggcg atcaccgcgt tcgagggatt gcgcgcggta cagcatggcg atggcgacct
     3001 gatcgcggtg tttcgcgcgc tgcgccttgc tccgcgcgcg ctggaggcgc tgtatcctct
     3061 ggcggcgaaa ttgccgccgg tgagcgagtt cttcgtcgat cctgccttgc gtgaagacgc
     3121 cggtctgctg tcgcggctgg cggagcccgc ccgcgacaac accggcatcg ttcacgacca
     3181 caacgaaccc ggcagccgcg gcggcttctc gctctatgtg ccggaatatt acacgcccga
     3241 ccgcgcctgg ccgctggtga tggcgctgca tggcggcagc ggcaacggcc gcggcttcct
     3301 gtggagctgg ctgcgcgatg cgcgcagcca cggcgccatc ctggtcgccc cgaccgcgac
     3361 cggcaatact tcaaacaaaa ctacctgggc cttgatgggc gaggataccg acagcccgaa
     3421 cctcgcccgc atcctcgatg cggtgcgcgg acgctggaac gttgatccgg caagattgct
     3481 gctgaccggg atgagcgacg gcggcacatt ctgctatgtc acgggcctcg agggcgcttc
     3541 gcccttcacc catctggcgc cggtggccgc caccttccat ccgctgatgg cggagatggc
     3601 cgatgcccgg agattgcggg gactgcccat ccatatcgtg catggccggc tcgactggat
     3661 gtttccggtg caggtggcgc ggaaggcgcg agaggcgctg acagccgccg gcgccgacgt
     3721 cacctaccgt gagctcgacg acctcagcca cgcctatccg cgcgagatga acgcgccgat
     3781 cctgaactgg ctgcgggctg agtgatcggc ccgctcacac catgccgcca ttggcgcgca
     3841 gcacctggcc gttgatccat ccgccgtcgg gaccgacgag gaaggcgacg gcggatgcga
     3901 tgtcctccgg cgtgccgaga cgctccagtg gattcatctt ggcaaaacga tcgaccaatt
     3961 cgggcgattt gccgtttaga aaaaggtcgg tcgcggtggg tccgggagcg accgcgttga
     4021 ccgtgatgtt gcgtccccgc agctccttcg acaggatcgc cgtcatggtt tccaccgccg
     4081 atttggtcgc ggcatagacg gcgtaggtct ccagcctggt cccgacgacg ctggtcgaga
     4141 aattgacgat ccggccgcca tcgcgcagcc gcctggcggc ctcgcgcatc gcgttgaagc
     4201 tgcccttgag attgaccgcg atctgctggt cgaacaacgc gtcgtcgctg tcggcgatcc
     4261 ttgcaagctt catgatcccg gcattgttga ccagcacgtc gacgccgccg aacgcggcct
     4321 cggtggtgtc gaacatgcca cgcacggcct ggggctcgct gacgtcggcc ctggcggtga
     4381 gcgcccggcc gcccttggct tcgaccttgc gggcgagcgt ctcggccgac ttcgtgtcgc
     4441 cggaataatt gatgacgacc atgaagccgt cggcggcgag ccgctccgcc accgccgcgc
     4501 caatgccgcg cgaggcgccg gtcacgattg cgaccttgct ggtcttgtcg gtcacgttct
     4561 tctccttatc ggcatggggc gctcccgcac attgatgcgc cgccacggac ggttctttca
     4621 agccttcgtg aagtccagcg cctgatgatt gtgtatactt gccgacataa tctcgtccga
     4681 atttttctcc agaagctaaa ggtttagcga gcagcggggc gctgccgcgt tggtttttga
     4741 accgcacgga gcccattatg cccccgaata ccgcctttgc atctcagccc cggccttccc
     4801 gctcgtctcc ctcacagctg ctgtcgaatg gcgtcaccct cctcgaaggc acgctgaaac
     4861 tcaccaccgc gatcctcgcg ctggcggccg gcgtctatac ctacctcggc gtccgcgaat
     4921 tgctgaacgg cagtccgacc acggtgttct ttgccgcggt gatctattcc gtggcggtat
     4981 cggtcggcat ctatgcgttc tgggtgttcc tgatgcagtt catgccgcat gtgctcgatc
     5041 gggccggccg tggcctgatg ttcgggtgca tgctgctggg atcgttgatg atcgtggcga
     5101 tgtcgtcctg gttgaacgcg tccgcactgg caggcgccgc cgcgatccag cagcatctcg
     5161 ccatcaccgt gcagaactat acccgcgacc tcgataccgc caacagcaac gccatcgcgg
     5221 cgcagggact gttgccggat atccagctgg cgtcgtcgcg ctttgccaaa cttgccgagg
     5281 ccgagcgtgc cggttcgctc accggcacct ccggatccgg caccgtggtg cagcttctga
     5341 cgcagatgtc cgggcagctc gacagtctcg gccaggaagt ccaggcctcc ggcaagcgcg
     5401 tctcggcgtt gtacgagcag ggcagcaaga acctcgccaa gatgcgcgaa ttgatcagcg
     5461 atcgcggacc gatttcgacg cgcagcgatg cctttgccgc cgagtcgctg gcgctgatgg
     5521 gcgtgatcgc caatttgcag cagacctcgg tagcgccggc ggtgaagcgc gccgcgacgt
     5581 cactgtcgtc gggcttcatc gcacccgccg cgggcggtcg cacggccgat ctggcggacc
     5641 ggcagaccgc cgtggtcggc aaggtggaga gttcgattgc cacgcaggcg gcgtcgctgt
     5701 cggacgccgc tgacaagatc ctggcgacgc cgcgggtcga accgatcagg tttcagtcca
     5761 tgtcgcccgc cgaggcggtg ctgcgctatg ccggcgattt cattccgagt tgggccggcg
     5821 cgatctcgat cgacctgatg ccggcggtgc tggtgcttat tctctgcatc gtgcatgccg
     5881 cgatccggcg cgaagaaatt cccgcgttca gcgcatccaa catgaccgcg ggcgaactgc
     5941 tcgcggcgtt gcagatggca cgcgaggtcg aggacgcgcg gcgaggaatt ccaaacgaga
     6001 tggtggcggc caccgcgaag gcggaaggag aacccgctgc cgcgccggcg ccgggcacgg
     6061 aggaaaacgt cacctcgctg tcgtcggcgc ggcttaccaa atagcggatc gccatgccgc
     6121 aatcttccag catgcaacaa cgctttttcg tctggctgtc gaacaatccg ggcgaagcga
     6181 ttctgcgctg gattttccgc gtcgtcgtca cggtcacgat cgccgcgctc gcggtcgacc
     6241 tcgccggcat gaacggctgg ggctggggcg ctcgccccga ccccgcagcg acgccagccg
     6301 agaccagcga tcttcccagc atcgtgccgt cgatccttgc gccgttgttg cccggcggtg
     6361 acaagcgtct cctgccgctg ccgccgccgg acggtccgat ggccaagccg atgacgttcg
     6421 agttggtcag cggcggcaag ttgatggcga ccggcaccat cacgccggga atctcggaag
     6481 cctttgccgc ggaagtcagc aggcacggcg aatacatcaa gaccgtggtg ctgagttcgc
     6541 ccggcggttc ggtcgccgat gcgttgacga tggggcggct cattcgcgag aagaaattcg
     6601 cgaccgaggt ggaagcggga aaattctgcg cgtcatcgtg tccgctggtg ttcgccgccg
     6661 gcgtcgagcg ccgcgccggc gaccgggccg cgatcggcgt gcaccaggtg gcggcgatca
     6721 gttcggcagc caacggtccg ccgcgcgacg agatgaaggt ggcgcaaaac atatcggcgc
     6781 gctgccagcg ctatctcgcc gacatgggcg tcaacctgca ggtctgggta cacgccatgg
     6841 agacgccgcc cgataagcta ttcgtcttca agccggatga gttgaaatcg ctcaatatgg
     6901 tgacggcaac acccgcctcg acgccggcag ccccacctgt gacgacatct gccgggacga
     6961 agacgcgttt gtgacgtagg tgtaacctct ccaccgtcgt cccggcgaca gggaactatc
     7021 ggcgcccgac cgcgttgtac gcgctcaatg gaggttgtca tgcaaacgtt tatcggaatg
     7081 atcctgggta tgctgttgct ggtcgcgggc gtctacatct acgactcgat ggcgacctcg
     7141 tcggtcgcca acggtcaggt cgcgcagccc aaccgtacca tcgtgaactg ggacgtcgca
     7201 gaggttaact ggaaggcact gaaggcgcgc gcccatgacg actggatcaa attgtcgtcc
     7261 aagtaaccgc gcaggtaacc gcccaagtca tcgcgccaca ttggcgtcgc aaggaccgaa
     7321 agcgctttcg ccatacggcg agggcgcttt tgctattcag cgctacttgt tcgccttcgc
     7381 tttccgcgca tcggcaatga cccgatcgaa ctccgccatg gtcaatgtgc ccggcacccg
     7441 aaacttgccg acgataaagg acggcgtgcc cttgaagccg aacgccgtcg cctgatcgtt
     7501 gttgcgcgcg atgatcgcgt cgattgcctt ggtgttggtg acgagatcgc ggtcgagacg
     7561 atccacatcg atacccgcgc cggcgagcaa ctcgcgcatg cgcggctcgg tcaatttcga
     7621 attgacgccg atcagcgcct catgggcctg aatatatttg tcctggaatt tgctcgccag
     7681 cgccatccgc gccgcgatca ccgaaaccgg ccccaggatc ggccagtcct tcaacaccag
     7741 gcggaccttg ccgtcatcct gcaccacctg ccgcagctcg ggctcgagct tgcggcagta
     7801 cgggcactgg tagtcgaaat attcgacgat cgtgatatcg ccatcgatat ttccggccgc
     7861 cgggatatcc gggtcgtgca gcaccagggc ttccgtcagg accgtttcgt cgtcgccttg
     7921 tccgagcgcc ggacccggcg cgacgcccag catcagtgta cccgcgccga gcaggccgag
     7981 cgcttcgcgc cgggtgcgcc gccggaccgg cggggctcga tcgatcgatt tgttcatgtc
     8041 tggtctccgg gccggcgcac ttatctacgt tcaggatagc ggtacgttac gcgccagcat
     8101 atagagcgcc accgcaataa tgacggcggc gaacacaatg ttgagcgcgc cgcggcgcac
     8161 cgacaagtgc cgcgcgaagc gggtaccgat cactccgccg gcgacgccac cggcgatgaa
     8221 taatccagcc agcccccatg agatcaatcc ggaccaggcg tagctggcgg cggtggtcag
     8281 gccgaacgcg gtcacggcga ccagcgacga actgaccgcg ttcatgatcg gcattcctgt
     8341 cgccagcatc agtgccggca cgatcagaaa tccaccgccg ataccgaaga agccggacag
     8401 cgtcccggtg gcaagaccga gcccgacaat ggcgggcatg ttggcccagt tcatctgcac
     8461 gtctggcaag cctgcgcgcg agcgcgtctt cagcatcagc atggcgatca ccagcatcag
     8521 cagaccgaac agcgccagca gcctctggcc gtcgaccatc ttgccgaaaa ccgagcccgc
     8581 gaacgcgccg gtaattcccg caaccgcgaa cggcagcgcg cacgaccaca tcacggtgcc
     8641 gccgcgcgca tgattcgaca gattgaccgc ggcgtttgcc gccaccgcga tggcgctggt
     8701 gccgatcgcg acatgggcgt cgggcacgcc gaccacatag accatcagcg gcaccgcgag
     8761 gatcgacccg ccgccgccga ccaggccgag cgaaaacccg accagcgctc ccgacgccag
     8821 tccgagcgcg ccctgcatcg cggagatcat cacgtcggta acctcagcac ggcgccccac
     8881 caaaacaaat ttttcgtaaa atctctatgg aggcgttgag gaaaaactgc aatgaggatc
     8941 agcgcaccag cagcgtcgcc gccagcggca ccagcaacga tgtcacgagc gcgttgagac
     9001 tcatggcgac gcttgcgaac acgcccgcga ccgcgtcaac ctgaaacgcg cgcgcggtgc
     9061 cgatgccatg tgcggcgatg ccaaccgcga atccgcgcgc gcgaaaatcc gtaatgccga
     9121 tccggttcat caatggcgtg acgatgatcg cgcccatgat gccggtcagg atcaccgcga
     9181 ccgccgtcag cgacggatcg gcgtgcaacg attcgctgat gcccatcgcg atacccgccg
     9241 taaccgattt cggcgccagc gacaagacca gcccacgcgg caggcccgcg gcctccgcca
     9301 gcagcaccac cgaaacgatc gccgtcacgg agccggccac cagcgccgcc agcatcggca
     9361 ggatcgccgc ggcaaccgtc ttgcggttct cgtagagcgg caccgccagc gcgacggtcg
     9421 ccggaccgag caggaagtgc acgaactgcg ccccgccgaa ataggtggtg tacgacgttc
     9481 ccgtcaacac caggaacgcc gcgatgatcc agatcgaatg cagcacggga ttggccagcg
     9541 gatgccgacg cgtcgccagc gatgccgcat ccgcggtcgc atagaccagc aacgtcaccg
     9601 tcagccagag cagcggcgac tgcgaaagat agacccacag cgagaacgga ttttcactca
     9661 tcgcgcgctc cgcccccgcg ccaacaggcg gctcgcgacg agaaacgtcg cgaccgtcac
     9721 cagcaaggtc aacgccaccg agatcgcgag aaccgccgcg attgcgatgc catggtcggc
     9781 gacgacatcc agcttctgca tgacaccgac tccagccggg acaaacagca gcgacagatg
     9841 cgcgagcaga cctcggctgg cattctccac gccgtcctgc tgcagcggcc cgcgcgcgag
     9901 aacgccgaag cggtcacgcg ccaacagcaa cagcagcagc aacaacaggc cgaccaccgg
     9961 acccggcatc ggcagcgaaa gaccgcgcac gatggcttcg cccgccagtt gacacagcaa
    10021 gataagactg aggcttgcga tcattggcaa tccgcgcgaa aaacgccggc atcaaccggt
    10081 cccggcgggc gcttgtcaat gtcgcttgat ggacccgcag cgcgaacccc gccgtctacg
    10141 catccgccgg ctcgggaaac gccagcagtt ttgccgcggt cctccggatg gtatcgacaa
    10201 aggcagtggg ccgccttacc ggtgatgata cggtagcgta tcagcatttt ggcggaccgg
    10261 ggcaatacgg taccgtatca agatcggcag gcagagcggc ctggcggccg aagagcggcc
    10321 gcgcaggccc ggtgaagggt tcagcccttc agcttcttgt tgcactcgct gtaatagccg
    10381 ccgcccttct ggatccattt caacccgcca ttggcgttgg cagtcttgtt ggcattgtat
    10441 tgatcgacgc aggta
//
LOCUS       DATNOI010000494        12867 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_728708,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000494 DATNOI010000000
VERSION     DATNOI010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12867
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728708"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            625..945
                     /locus_tag="VN968_19855"
     CDS             625..945
                     /locus_tag="VN968_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31525.1"
                     /translation="MNELRMKMAPMRKALVAAGAGVGLLAFSTVSASAAIACRDDVCW
                     RVHERYVYPPSPTIIIHPDDWRPPTARIIIHPEGWRPVERFTREYVVRDYYGYGYGYG
                     DPDW"
     gene            complement(1266..1487)
                     /locus_tag="VN968_19860"
     CDS             complement(1266..1487)
                     /locus_tag="VN968_19860"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31526.1"
                     /translation="MALLFFNVGVETGQLVFVAAVLSLIWLLGHAASQLLAAALVKRA
                     FDRLDVTIAYGIGAVAAYWLIERTTAFFA"
     gene            2040..3221
                     /locus_tag="VN968_19865"
     CDS             2040..3221
                     /locus_tag="VN968_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXL31527.1"
                     /translation="MSADEPDFVAFQDQPISQLLVKVAARCNIDCSYCYWFRDAAVYD
                     KPKLMSADVLHRLLQRIEEHVAKHSLIDFPIILHGGEPLLWGIENFNRMAEACEAISS
                     RTGCEIPIAVTTNGVLIDDEWLNCFEAHNISVAISLDGPAHIHDIHRRTFQGTGTHAA
                     VERAARMLVSRDIGVSALAVCNPAYLPEQYVKFFAECGVANYDIMIPDATVDENPPSI
                     ASFYTGLFDLWLEANRSKPTVNIRIIGDMITALLGNNSPTEGVGHKPVELCTVMTDGS
                     VEAHDVLRIAGDGFTRTMFNIFDHAIDEVRGEPRWKAARDASINLCQKCQQCKFMNAC
                     GGGYLPHRFSRKNGYDNPSVYCGDLYSMFENMQSVLESHLYVSKPGGERLDMRDALGR
                     A"
     gene            complement(3519..4445)
                     /locus_tag="VN968_19870"
     CDS             complement(3519..4445)
                     /locus_tag="VN968_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL31528.1"
                     /translation="MMRLKLILTATALCVSGHAAIAQEPAGTLEKIRSTGIITIGHRE
                     SSIPFSYYDKNEKVVGYAVDLCYLVADAVKASLGLAKLEVKLVPITPSLRIPSILSGK
                     IDLACETMTNNLERQKVVAFSSTYFVAANRFVSKATANLRTLDDLKGRTVVSTIGSTN
                     LRQISELNAQRNLGLTIVAAKDNFEAFRMLESDRAAAHFMDDILLYGAVANSATPADY
                     VVSEQALSVEPYGILLPRDDAAFKKVADDTLSAIYRSGEINKIYAKWFLKPIPPNGIS
                     LNVPMSAALKRVIEKPTDNGDPEAYAIRNAAQ"
     gene            complement(4484..7210)
                     /locus_tag="VN968_19875"
     CDS             complement(4484..7210)
                     /locus_tag="VN968_19875"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXL31529.1"
                     /translation="MSWAARLRTGLVANSALAGGARRTASTRPIVVLVLCGAFLIALV
                     VGVIALILSNLRHHAIEQSKQQLMATATVLARQAARDFQSIDLIEANLIEYMETLAIV
                     SDELYARAMVGRGIHLMLRDKISGFPHIGAVMLIGADGGLINSSQAWPTPPLNFAGQD
                     YFEALKASPRLDSMLGEPTQDRQTGVWTISFARKFRGSNGEFLGLVVGTVASQYFEES
                     YATLARGTDESIALFRHDGVMLARYPHIDSSIGKSVVRNLPDGKTQRSGSGVVGQREV
                     VDDSERLIAEARLPNVPIYLTASTTVTAALADWRTEAGYLIGLTIVLVVAIGGIGAVV
                     VQFFRGQSIQLDSTLNNLSQGVCMYDEHRRLILCNDRYAEMFHLPHELTMPGTALRRI
                     FDHQVAHGLHPESGPDEYIGGMMAIITAGEPASTVTEFSDGRAMVAVFRPAPGGGFVT
                     TVEDITEQRRAEKRVAHIAHHDSLTGLHNRAAFSDYLATAIDETLRAAGTFAILCLDL
                     DRFKEVNDLYGHLVGDALLCEVARRLQAVAEGAFLARVGGDEFVVITVGDAQAASVGQ
                     LALQLRAALSGDIELAGRQLRIGLSIGIASCPVDGTDPATLLANADVALYRAKAEGRD
                     TIRFFEPDMAAQLRDRRELQHDLQSALANDELRLDFQPLARIDGEIVGFEALVRWHHP
                     SRGVVAPGIFIPLTEEDGLIVAMGEWILAAACREAVSWPNQLQISVNLSPVQFRNDDI
                     VRLVHETLIETGLDAGRLELEITEGVLIDDFSRAVSILRRLKSLGVRIALDDFGTGYS
                     SMSYLQAFPFDMIKIDRSFISNLERSAQSKALLRGVIGLARGLELPVTAEGVETRAQL
                     EVLTRAGCDLVQGFLIGRPASIDGYAEIVGRPASPERRGMPASQASTARGAR"
     gene            complement(7618..8391)
                     /locus_tag="VN968_19880"
     CDS             complement(7618..8391)
                     /locus_tag="VN968_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL31530.1"
                     /translation="MSDPSRSEFGDFLRSRRAKLNPKSVGLPDGRRRRAPGLRREEVA
                     ELAGIGVDWYVRLEQGRAVSPSVTTIDALARALRLSKVEHAHLRALASGVNRRAFTRE
                     NVPAPLRNLVESLNQPAYITGRRWDLLAWNKAAAEIFAFDRLAEEDRNILVCIFLFPG
                     TRRLFGSSWAEEAKRVVAQFRATHDLWAGDPAFIELLTRLRNGSPEFAEWWETHDIRG
                     GAGGVKRLRHPKKGMLRLAHASFQANDDPDLKLIIYTPA"
     gene            8586..9056
                     /locus_tag="VN968_19885"
     CDS             8586..9056
                     /locus_tag="VN968_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL31531.1"
                     /translation="MSAGSSIHVSLKINGRDHEVSAPGCDTLLDVLRDKLGMTGTKKG
                     CDMGACGACTVLADGRRIKSCLALAASCDGRSITTIEGIGTLDNLHPLQAAFIRHDAL
                     QCGYCTPGQILSGLALLDEKPADLAAIREGMSGNLCRCGAHPNIVAAIAEVAGL"
     gene            9053..10042
                     /locus_tag="VN968_19890"
     CDS             9053..10042
                     /locus_tag="VN968_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXL31532.1"
                     /translation="MSSPSFQYSRASDVSDAFIQGSRAGTAFVAGGTDLLQLWKAGAM
                     APEGVVDISRLPLGLVEYVDGQLSLGALARLSDVAAHPDVARDHPVIAEAILASASGQ
                     IRNMATVGGNLLQRTRCPYFRSQGLACNKRLPGSGCGALSGENRQAALFGASTSCVAT
                     HASDLAVALMALDAEVEVFGAAGRRRLPLRDLYRLPAETPERETDLAAGELITGIHVA
                     NASRFAARHTYLKVRDRASFEFAVVSVAAVLRVENGAIVEARLSAGGVAPMPWRLARS
                     EAALVGRAPAAEAFAAAGDLAIEDARPLAHNGFKIELLRNAVIRALQTAGGRP"
     gene            10039..12210
                     /locus_tag="VN968_19895"
     CDS             10039..12210
                     /locus_tag="VN968_19895"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXL31533.1"
                     /translation="MSTDSALRYEAALKVTGSAVFEAEVPAAGMLHAALVEAPIGCGD
                     VLSVDATPASALPGFVAMISYREADALQPSPATALIRERAIHFAGQPVALVTAGTWLE
                     ARSAARAVRIATQARPAVTGMAQAIDQSFAPAMVGRFPAESRRGDAATALAEASLVVR
                     HQYVTAVNNHHPMEPHAVVCWWEGGKAVVHTSTQAIFGTRAMIAHAFRMPTADIRVFS
                     RFLGGGFGCKGQLWWPWMFWAMLAAKRTGRPVRLELTRAQMFSLIGRRQQTVQDLALG
                     FGADGRLTGIDHRVVAQTSTHADYSDATAVYSRWLYACPNVTTTHRLVRTNEPHPVPM
                     RAPGTAPGTFALESAFDEAAERLGIDPVELRVRNFADHDQEAGRPWSSNGLLECYRIG
                     AERFGWSARPARGAAIDGRWRIGWGMASTLYPAIRQACKVRVNLAADASLRVQCGTQD
                     MGSGTYTVLGQMAATALGVSMSQVTVELGDSWLPEGPFSGGSQVTASMAPAVEMATAR
                     LRATLARMAVADDRSPLSGQRIEDLDFQDGMIRSRTGNVGELLTDVLARGAADGLEAA
                     GESAATQLQSLAVTSMGYGAVFVEVGVDPELGEIRVRRVCGAFAAGRILNPRLAKSQY
                     VGGLIGGIGMALHEMTVTDRASGRILGDTFADYLIPVHADVPEFDITMVDEEDPHLPG
                     SVKGIGMLGTAGIQAAIANAVFDAIGKRVRSLPIRIEHVIG"
     gene            complement(12416..>12867)
                     /locus_tag="VN968_19900"
     CDS             complement(12416..>12867)
                     /locus_tag="VN968_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ring-opening amidohydrolase"
                     /protein_id="HXL31534.1"
                     /translation="FSSVASTSAGIELMHNVVIVLGNSTSSASPFEIGHAVMRDAIDS
                     ASVIDALKSVGLRVGDGSEPTAGRQLVNIFAKAEASPNGTLRGFRHTMLEDTDISATR
                     HARAAVGGLIAGLSGTGAVYVSGGAEHQGPPGGGPVAAIARLSKTSD"
BASE COUNT         2458 a         3910 c         4038 g         2461 t
ORIGIN      
        1 atcggtcttc ccggcgcatg cgaacactgg tgacccaaat ggcccccgtc gaggcctgtc
       61 cgctaatcag aacgcaggcg cgctgatatc catgatggcc cggagcaaat gctccaatca
      121 aaggccggat acattgatgc acgaccgctt accaccagat cgacgaaaac ctcttgcaac
      181 gcgcggccgg accatacatt cgggtcaaac tgagaagaac tcagtgtgag caaatgtctt
      241 ccgcgttagt cgcgcgcaaa acaccacggc tgtacgggcg gtcagggcct tacgccactt
      301 cctacgatgg ctggcatcgg aactcggacc ggcatctcag atttgaggaa gatcaactcg
      361 gaatccgggc gattgtgcgg tggctcggtc tggccgataa taccgagcgc atagaccttg
      421 attgccgaaa gcatgcgcga tcgcaatggc aagtggctag tgatccccga ctaagcccct
      481 ggtgcccgcg taaagtgttt tttttgcccg gttaccttac ccacaggggc agttttaatg
      541 ttcttccacg gaaacgaagc gaggtctgcg ggcgtttccg tctcgggctg caaaaaaaga
      601 agaagcccaa aggaggagga agatatgaat gagctgagaa tgaaaatggc gccaatgaga
      661 aaagctctcg tggcagctgg cgcgggtgtc ggccttctgg cgttctccac agtgagcgcc
      721 tcggctgcca tcgcatgcag ggacgacgtt tgctggcgtg tccacgagcg ctatgtctac
      781 ccgccgtccc ctacgattat tatccatccc gacgattgga ggccgccgac tgcgagaatc
      841 atcattcatc cagaaggctg gcgtccggtc gaacgcttca cgcgtgaata cgtcgttcgt
      901 gactattacg gttacggcta cggttacggc gatccagact ggtaatctac ccgagaggcc
      961 gcttcctcag cggccctctc ttgttggccg ttcacacccg tccttcgatg gccacgggtt
     1021 cctgaaatcc gaaattccgt tcggcttaaa ccaactcggc tgtgcgcctc ttggcgctgc
     1081 cacattgtcc tgaaactcct cggtgtggtc cgttatcccg aatgcgtggc gttgatcatc
     1141 cagatcggca accatctcag caagcagctt gatgtgaagg ggtgagggcg ttgggtccac
     1201 cggttgaggc caccgctaga aacgggcggc acgtcttcgc agcgttgagc gcatcacggt
     1261 cgtcgtcatg caaaaaaggc ggtcgtccgc tcaatcagcc agtaggcggc aacggcgccg
     1321 atgccgtagg cgatggtcac atcgagccgg tcaaatgcgc gcttgacgag ggcggccgcc
     1381 aatagctgtg acgcagcgtg gcctaacaac cagatcaatg acagcaccgc ggccacgaag
     1441 acaagctgcc cggtctccac gccgacattg aaaaacagca gcgccaccgc ccgtcgccaa
     1501 cggcgaggaa atcactggct ccagcggcca gcaagtcaaa ctcctgtccc gttgattcat
     1561 gggatcacat tggtctcact gagttgggcg ccaaatgcta cgtcgacgga acaaaccttc
     1621 acaaaaccag acgcacgcca cgtttgccac ggcgatggaa aaggtatagg atttattttc
     1681 gctcagctgt ttgtatatcc cgacctttcg ttcgatccat ttggttgcgt cgcaaagaag
     1741 gatatcatca tgccgatcac gacgttgaag gttttagcga gcgtcctgca actgcacggg
     1801 ccaggcgcaa caattcagag cgatcatcaa ggcatcgatg caatactgag tgatctgcgc
     1861 ggacaaaatt cggaaggcgt gacgcaggtt tatttcagac gaggcggttt tgtccgaggg
     1921 gggtatgggg gcggctttcg cagagcaggg tatcatggag ggggcgggtt tcgcagaggc
     1981 gggtttatcc gagggtatta gatttacttg gtaatttcct cgtacgcact ggtttaggca
     2041 tgtcggctga cgaaccagat tttgtcgcct tccaagatca gccaatctcg cagctgcttg
     2101 ttaaggttgc ggccagatgc aacatcgact gctcttattg ttactggttc cgcgacgcgg
     2161 ccgtttatga caagcccaag ctcatgagcg ccgacgtgct gcatcggttg ttgcagcgca
     2221 tcgaggagca cgttgccaaa cattccctga tcgattttcc aatcatcttg cacggcggcg
     2281 agcccttgct gtggggaatt gagaatttta atcgtatggc cgaggcctgc gaagccatat
     2341 catcacgaac gggctgcgaa atacccatcg cggtgaccac caatggcgtg ttgattgacg
     2401 atgaatggct gaactgtttt gaggctcaca acatttcggt tgcaatcagc ctggatgggc
     2461 cggcgcacat tcacgacatt caccgccgga cgtttcaggg tacaggcaca cacgccgcgg
     2521 tggagcgggc cgctcgcatg cttgtatcac gggacattgg cgtgagcgct ctggcagtct
     2581 gcaatcccgc ctacctgccg gagcagtatg tcaagttttt cgctgagtgc ggagttgcca
     2641 actacgacat tatgattcct gatgcgacgg tagatgagaa cccgccatcc attgcctcgt
     2701 tctacacggg cctattcgat ttatggctgg aggcaaaccg cagcaagcca acggtcaata
     2761 ttcgcatcat tggcgatatg atcaccgcct tgctcggcaa caattcaccg accgagggcg
     2821 taggccacaa acctgtcgaa ctttgcacag tcatgaccga cggatccgtg gaggcccacg
     2881 atgtgctgcg gattgccggc gacggcttca cgcgtacgat gttcaacatt ttcgatcatg
     2941 cgatcgacga ggtcaggggc gagcctcgct ggaaagccgc gcgcgatgct tcaatcaatc
     3001 tttgccaaaa gtgccagcag tgcaagttca tgaacgcctg cggcggcggt tatcttccgc
     3061 atcgtttttc caggaagaac ggttacgaca atccctcggt ctattgcggc gatctctatt
     3121 caatgtttga gaacatgcaa tctgtattgg agagtcatct ttacgtcagc aagccaggcg
     3181 gggaacgcct cgacatgcgg gacgcgttgg gacgcgccta gtacggtctt cgcaccatcg
     3241 cgggcggtgg tgcgcgatcc tccggcaaga gtagcggcgc ggtggtcgcg tccgggcagg
     3301 gtcacaagcg ggctatcgat tgcgcgagtg aggatgcgat gttattcggt tctcgaatgg
     3361 cgtccggttg aacgcgggtc agttgctcga tgaaagcgct cagaccgcct aggtaatttg
     3421 gaaggtccgc taacggcgag ccgtgaccga tgcattcaat ccgaccaccg ctcgcgcttc
     3481 accggcccgg tcgccgggcg ccccgcgaac ttgcccgatc attgcgccgc atttcgtatc
     3541 gcatacgctt ccggatcacc gttatcggtc ggtttctcga tcacccgctt cagcgctgcg
     3601 ctcatcggga cgttgaggct gatgccgttc ggaggtatcg gtttgagaaa ccatttggca
     3661 tagatcttgt tgatctcacc gctgcgatag atcgccgaca gcgtatcgtc ggccaccttc
     3721 ttgaaggcgg catcgtcgcg cggcagcagg atgccatacg gctcgaccga cagcgcctgc
     3781 tccgacacca cgtagtcggc gggcgtcgcc gagttggcca cggcgccgta cagcaggata
     3841 tcatccatga agtgtgcggc tgcgcgatcg gattccagca tgcggaacgc ctcgaaattg
     3901 tccttggccg cgacgatggt gaggccaaga ttgcgctgcg cgttgagctc gctgatctgc
     3961 cggagattgg ttgatccgat ggtcgacacc accgttctgc ccttgaggtc gtccagggtc
     4021 cgcagattgg cggtcgcctt ggagacgaag cggttggcgg cgacgaaata ggtcgatgag
     4081 aatgccacga ccttttgccg ttcgaggttg ttggtcatgg tttcgcaggc gagatcgatc
     4141 ttcccgctca ggatcgacgg aatgcgaagc gacggcgtga tcggcaccag cttgacctcc
     4201 agctttgcca ggccaaggct ggccttcacg gcgtcggcaa cgaggtagca gaggtccacc
     4261 gcatagccca cgaccttctc gttcttgtcg taataagaga aggggatcga cgattcacga
     4321 tgtccgatcg tgatgattcc ggtgctccgg atcttctcca gtgtccccgc cggctcctga
     4381 gcgatcgccg catggcctga gacgcacagc gcagttgcgg tcaggatcag tttcaagcgc
     4441 atcattccat ctcctcgttg ggatttccgg ttcatcttca cattcatcgc gcgccccgcg
     4501 ccgttgacgc ctggctcgcc ggcatgccgc gccgctccgg gcttgccggc cggcccacga
     4561 tctccgcata gccgtcgatc gacgccggcc ttccgatcag gaagccctgc accagatcgc
     4621 agccggcacg ggtgaggacc tcgagctggg ctcgcgtctc gacgccctcg gcggtcaccg
     4681 gaagctcgag cccgcgggcc agtccgatca cgccacgcaa cagggccttc gattgcgcgc
     4741 tgcgttcaag gttcgatatg aagctgcggt cgatcttgat catgtcgaac ggaaacgcct
     4801 gcagatacga catcgacgag tagccggtgc caaaatcgtc gagcgcaatg cgcacgccca
     4861 gcgatttgag acgccgcagg atcgacaccg cgcgggagaa gtcgtcgatc agaacgcctt
     4921 ccgtgatctc gagctcgagc cgcccggcgt ccaatccggt ctcgatcaga gtctcgtgga
     4981 caagccgcac gatgtcgtcg ttgcgaaact gcaccggcga caaattgacg gaaatctgca
     5041 attggttcgg ccaggacacg gcctcgcggc acgcggcagc caggatccat tcccccatcg
     5101 cgacgatcag cccgtcctct tccgtgagtg gaatgaagat tccgggtgcg accacgccgc
     5161 gactcggatg gtgccagcgg accagcgctt cgaaacccac gatctcgccg tcgatcctcg
     5221 ccagcggctg aaaatcgagc cgcagttcat cgttggcgag agcagactgc agatcgtgct
     5281 gcaattcgcg tcgatcgcgc aattgcgcag ccatgtcggg ctcgaagaat cggatcgtgt
     5341 cgcgcccttc ggccttggcg cggtagagtg ccacatccgc gttggcgagc agggtcgccg
     5401 ggtcggtccc atcaaccgga cacgacgcga tgccgatgct cagtccaatg cgcagctgtc
     5461 gtccggcgag ctcgatgtca ccgctcagcg cggccctgag ttgcagagcc agctgaccca
     5521 cggacgcggc ctgcgcgtcg ccgacggtga tcacgacgaa ctcgtcgccg ccaacgcgtg
     5581 ccaggaaggc cccttccgca accgcttgca gacgccgcgc cacctcgcac agcagcgcat
     5641 cgccgaccag atggccgtag aggtcgttga cctccttgaa gcggtcgagg tcgaggcaca
     5701 ggatcgcgaa cgtcccggcc gcgcgcagag tctcatcgat ggccgtcgcc agatagtcgg
     5761 aaaacgccgc gcgattgtgc aggccggtca gtgagtcgtg atgagcgatg tgcgcgaccc
     5821 gcttctcggc gcgcctttgc tcggtgatat cctccacggt ggtgacgaag ccaccacctg
     5881 gcgccggccg gaacacggcc accatggccc gcccgtcgga aaattccgtc acggtgctcg
     5941 ccggctcgcc ggcggtgatg atagccatca ttccgccgat atactcgtcg ggtccacttt
     6001 ccggatgcag gccgtgcgca acctgatggt cgaagatccg gcgcaacgcg gttcccggca
     6061 tcgtcagctc gtgcggcagg tggaacatct cggcatagcg atcgttgcac aggatgagac
     6121 gccggtgttc gtcgtacatg cagacgcctt gcgacagatt gttcagcgtg gagtccagct
     6181 ggatgctttg cccgcggaaa aactgcacga cgacggcgcc gatgccgccg atcgccacca
     6241 cgaggacaat ggtgagcccg atcagatacc cggcctcggt ccgccagtcg gcgagcgcgg
     6301 cggttaccgt cgtactggcc gtcagataga tcggaacgtt gggaagccgc gcttccgcta
     6361 tcagccgctc ggaatcatcc accacttcgc gctgcccaac gacgccgctg ccggaccgct
     6421 gcgtcttccc gtcaggcaag ttgcggacaa cggattttcc gatggaggaa tcgatatgcg
     6481 gatagcgcgc gagcattacg ccatcgtgac gaaacagcgc gatcgactca tcggtcccgc
     6541 gcgcgagcgt tgcataggat tcctcgaagt actgcgatgc gaccgtgccg acgaccaggc
     6601 cgaggaattc gccgttcgat cccctgaatt tacgggcgaa ggaaatggtc cagacgccgg
     6661 tctggcgatc ctgcgtcggt tcgccgagca tcgagtccaa tcgcgggctg gccttcagcg
     6721 cctcaaaata gtcctgacct gcgaaattga ggggtggggt tggccaggcc tgcgacgaat
     6781 tgatgagtcc gccatcggcg ccgatcagca tgaccgcacc gatgtgaggg aagccgctga
     6841 tcttgtccct cagcatcagg tggatgccgc gtcccaccat ggcgcgggca tagagctcat
     6901 cggagacgat cgcgagcgtt tccatgtatt cgatcaggtt ggcctcgatg aggtcgatcg
     6961 actggaagtc acgcgccgcc tgccgggcaa gcacggttgc ggtcgccatc agctgctgct
     7021 tgctctgttc gatcgcgtga tgacgcaggt tggacaggat cagggcgatc acgccgacga
     7081 ccagcgcgat caggaatgcg ccgcatagca cgaggacgac aatcgggcgc gtcgaggcgg
     7141 tccggcgcgc tccgcccgcc aacgcggagt tcgctaccag gccggtacgc aagcgagcag
     7201 cccacgacat gattcccgcg gtggtcgaca tcccggaaac actgggtccg gctgcttgga
     7261 atcgcgttaa cagcgaggct ttggccgcgc cggaacctcg gcacggttaa gaatgcatgt
     7321 tatcgccgcg cttgcggccc aaaggcggac caggctgccg ctttgttgga cagcatcgcg
     7381 ccgctggccg ggtattatta cgggcaaatc aatcggccgc ccgcgagcta cggcagggcg
     7441 gccttgcgga tgtcgtgccc tcaggtgcgt ttggttagca atggattgcc gcgggcacac
     7501 acaaagcgtc tgaactcgta atcgataaaa gcccgatcag tgtgcgcgga tgtccataac
     7561 atgagagggg acggttggct cattgcaaag cttgtcatcg tcggccgcga aacggtttca
     7621 agccggtgta tagatgatga gcttcaggtc gggatcgtca ttggcctgga aacttgcgtg
     7681 cgcaagacgc agcatgccct tcttgggatg tcgcaagcgc ttcacgccgc ccgcgccgcc
     7741 tctgatatcg tgggtttccc accattctgc aaactccggg ctgccgtttc gaagacgggt
     7801 tagcagttcg ataaacgccg gatcgccagc ccagagatcg tgtgtcgcac gaaactgggc
     7861 gacaacacgc ttggcctcct cggcccagct tgatccgaac aggcgccgcg ttcccggaaa
     7921 aagaaatatg cagacgagaa tgttgcgatc ttcttccgca agacgatcga aggcgaaaat
     7981 ctcggcggca gccttgttcc aggcaaggag gtcccagcgc cggcccgtga tgtaagccgg
     8041 ttggttcagg gactcgacaa ggtttcgaag gggggcggga acgttttccc gggtaaaggc
     8101 gcgccggttt actcccgacg cgagcgcacg caaatgcgcg tgctctactt tgctgaggcg
     8161 caacgcacgt gccagcgcgt caatcgtggt cactgaagga ctaacggccc gtccttgttc
     8221 cagccggacg taccagtcca cgccgatgcc cgcgagttcg gcgacttcct cgcgccgcaa
     8281 gccgggagcg cggcgccttc ggccatcggg aaggccgacc gacttcggat tgagcttggc
     8341 gcgccgcgaa cgaagaaaat caccgaactc gcttcggctg gggtctgaca tggcctttta
     8401 tctcccgcga ggaggtactc tcagtcctag gataatacta gtccttccgg ggcgcgcgca
     8461 atctgcgatg atggcggaat gcggggcgga tcgaccgccg ctcgcttttg ccatgacagc
     8521 cggacctctg accaccgggg gatgagcttt gatcaagctc cggcaagaca atttcaagga
     8581 acttcatgtc agctggttcg tccatccatg tttcgctcaa gataaacggc cgggatcacg
     8641 aagtctctgc tccaggctgc gacacgctcc tggatgttct ccgggacaag cttggcatga
     8701 ccggcaccaa aaaaggctgc gacatgggag catgtggagc atgcaccgtg ctggcggacg
     8761 gacgccggat caagtcctgt ctcgcgctcg cggcttcctg tgatggccgg tcgatcacca
     8821 cgatcgaggg catcgggaca ttggacaacc tgcatccgct gcaagccgct ttcattcgtc
     8881 acgacgcgct gcaatgcggc tattgcacgc ccgggcagat tctttcaggc ttggcgctac
     8941 tcgacgaaaa gcccgcggat ctcgccgcca ttcgcgaggg catgagcggc aatctctgcc
     9001 gatgcggagc ccatccaaat atcgtggcgg cgattgccga ggtagcggga ctatgagttc
     9061 accctcgttt cagtacagtc gtgcttcaga cgtcagcgac gccttcatcc agggttcgag
     9121 agcaggcacc gccttcgtgg ccggcggaac cgacttgctg cagctttgga aagcaggcgc
     9181 gatggcgcct gagggggtcg tcgacatctc acgcctgccg ctgggtctcg tcgaatacgt
     9241 cgacgggcag ctatccttgg gcgcgctggc ccggctgagc gatgtagccg cccacccgga
     9301 tgtggcgcgg gatcatccgg tgatcgccga ggcgatcctg gccagcgcga gtggacagat
     9361 tcgcaacatg gcgacggtgg gcggcaatct gctccaacgc acccgctgcc cgtattttcg
     9421 cagccaagga ctcgcctgca acaagcgttt gcccggttct ggatgtggcg cgctttccgg
     9481 tgagaaccgt caggctgcct tgttcggcgc aagtacgtcg tgcgtcgcga cccacgcctc
     9541 ggacttggcc gtggcgctga tggcattgga tgccgaggtc gaggtcttcg gagcggctgg
     9601 acggaggcgc ttgccactgc gcgatctcta ccggctgccc gccgagacac cggagcgcga
     9661 aaccgatctg gcggcaggag agctcatcac cggcattcat gtcgccaacg catcccgctt
     9721 tgcggcgcgc cacacctatc tcaaggttcg cgaccgcgca tccttcgagt tcgccgtggt
     9781 atcggtggca gcggtactgc gcgtcgaaaa tggtgcgatt gtcgaggcga gattgagcgc
     9841 cggcggggtc gcgccgatgc cctggcggct tgcgcgcagc gaggcggcac tggtcggccg
     9901 cgcgcccgcc gccgaggcct tcgctgccgc aggcgatctg gcgatcgagg acgcccggcc
     9961 actcgcccat aacggattca agatcgaatt gctgcgcaat gccgtcatcc gcgccttgca
    10021 aaccgctgga ggccggccat gagtaccgat tccgctcttc gctacgaggc cgcgctgaag
    10081 gtcaccggca gcgccgtctt cgaggccgag gtgccggcgg caggcatgct gcacgccgcg
    10141 ctggtggaag cgccgatcgg ttgcggcgac gtgctgtccg tcgacgcaac accggcaagc
    10201 gcgctgccgg gcttcgtcgc catgatctcc taccgggaag cagacgcgct gcagccgtcg
    10261 ccagcaaccg ccctgatccg ggagcgcgcc attcactttg cgggccagcc ggtggcactc
    10321 gtgaccgcag gcacatggct cgaggcgagg tcggcggcgc gcgcggtccg gatcgccacc
    10381 caggcacgtc cggcagtgac ggggatggcg caggcgatcg atcagtcatt tgcgccggcc
    10441 atggtcggac gctttcccgc cgagagccgc cgcggagacg ctgccacggc actggccgaa
    10501 gccagtctgg tcgtccgcca tcaatacgtg acggccgtca acaaccatca cccgatggag
    10561 ccgcatgcgg tggtgtgctg gtgggagggc ggcaaggctg ttgttcacac ctcgacgcaa
    10621 gccatcttcg gcacgcgggc gatgattgcg cacgccttca ggatgccgac ggcagacatt
    10681 cgggttttct cgcgcttcct tggcggcggc ttcggctgca aggggcagct ctggtggccc
    10741 tggatgtttt gggcgatgct ggcggcgaag cgcaccggcc ggccggtccg gctggaattg
    10801 acgcgcgcgc agatgttcag cctgatcggc cgccgccaac agacggtgca ggatctggcg
    10861 ctggggttcg gcgcggacgg ccgtctcacc ggcatcgatc atcgcgtggt cgcgcagacc
    10921 tcgacgcatg ccgattactc ggatgccacg gcggtctact cgcgctggct ctatgcctgc
    10981 cccaatgtga cgacgacgca ccgtctcgtc cgcaccaatg agccgcaccc ggtcccgatg
    11041 cgcgccccgg gtacggcacc cggcacgttt gcgctcgaat ccgccttcga cgaggcggcc
    11101 gaacggttgg ggatcgatcc ggtcgaacta cgcgtgcgga attttgccga tcacgatcag
    11161 gaggccggcc gcccgtggag cagcaatggc ctgctcgaat gttatcgcat cggcgccgaa
    11221 cggttcggct ggtcggcgcg gccggcgagg ggcgccgcca tcgatggacg ctggcggatc
    11281 ggctggggca tggcgagcac cctttatccg gcgatccgcc aagcctgcaa ggtccgcgtc
    11341 aatctggcgg cggacgcgtc cttgcgggtt caatgcggaa cccaggacat gggttcgggc
    11401 acctatacgg tgcttggcca gatggcggct acggcgctcg gcgtttccat gtcgcaggtg
    11461 acggtcgagt taggcgatag ctggttgccg gaagggccgt tttccggcgg ctcccaggtg
    11521 accgccagca tggcgcccgc ggttgaaatg gcgaccgcca ggctccgcgc gacgctggca
    11581 cggatggccg tggccgacga ccggtcgccg ctctccggtc agcggatcga ggacctcgat
    11641 tttcaggacg ggatgatcag gagccggacc ggcaatgtgg gcgagcttct gacggatgtg
    11701 ctggcgcgcg gtgcagctga cgggttggaa gcagcgggcg aaagtgccgc aacccaactc
    11761 caatccctgg ccgtcaccag catgggctac ggcgcggtgt tcgtcgaagt gggcgtcgat
    11821 ccggagctcg gcgagatccg ggtgcgtcgg gtgtgcggcg cctttgctgc cggccgcatc
    11881 ctcaatccgc gtcttgcgaa gagccagtat gtcggcggcc tgattggcgg catcggcatg
    11941 gcgctgcacg agatgaccgt gacggatcgc gcttcaggcc gcattctggg cgacaccttc
    12001 gctgattacc tgatccccgt tcatgccgac gtgccggagt tcgatatcac gatggtcgac
    12061 gaagaggacc cgcacctgcc gggcagcgtc aagggtatcg gcatgctggg cacggcgggc
    12121 atccaggcgg ccatcgccaa cgcggttttt gatgcgatcg gcaagcgagt tcgcagtctg
    12181 cccatccgga tcgaacatgt gatcggataa gagcctgagc gaagcagggc atgcagcagg
    12241 ccaagtcagg gtcgaatgga cacaaaaggt tctgggatca ccaggctaag gtaccgcaaa
    12301 aggatggcac cacgtcaaaa gccgactttc aggaatcccc gcctttgcgg atttagtcct
    12361 ccggcgcaaa gcggacattg atcgaagcta tcggcaaagt gcctcaaaga gacattcagt
    12421 cggaagtctt tgatagccga gctattgcag cgacggggcc gccgccgggc ggaccctgat
    12481 gttcggctcc gccggaaaca tagacggcgc cagttcctga taagccggcg atcaacccgc
    12541 caacagcggc gcgggcgtgt cgggtagcgc tgatgtccgt atcctcaagc atggtgtgac
    12601 gaaatccgcg cagggtgccg tttggcgagg cttccgcctt ggcgaagatg ttaaccagtt
    12661 gacggcctgc cgtcggctcg gagccgtctc caacgcgaag tcccacgctc ttcaacgcat
    12721 cgatcaccga ggcggaatcg attgcgtcgc gcatgacggc gtgtccgatc tcaaacggac
    12781 tcgccgacga tgtcgaatta cccaagacga tgaccacatt atgcatgagc tcgataccag
    12841 ccgacgtgga tgccaccgac gaaaata
//
LOCUS       DATNOI010000495         4703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2577018,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000495 DATNOI010000000
VERSION     DATNOI010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2577018"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VN968_19905"
     CDS             complement(<1..155)
                     /locus_tag="VN968_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HXL31535.1"
                     /translation="MFKVRVIPCLDVKDGRVVKGVNFVDLRDAGDPVEAAIAYDAAGA
                     DELCFLD"
     gene            complement(292..1032)
                     /gene="hisA"
                     /locus_tag="VN968_19910"
     CDS             complement(292..1032)
                     /gene="hisA"
                     /locus_tag="VN968_19910"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HXL31536.1"
                     /translation="MILFPAIDLKNGQCVRLEQGDMARATVFNLDPTAQARSFAAQGF
                     EYLHVVDLDGAFAGKPMNAHVVEAMLRAVTMPLQLGGGIRDLNTIEAWLAKGVARVII
                     GTAAVRDPDLVKGAAKKFPGRVAVGLDARDGKVAVEGWAETSEVTALEIARRFEDAGV
                     AAIIFTDIARDGLLKGLNLDATIEIAERISIPVIASGGFASIEDVRALLTPRAKKLAG
                     AIAGRALYDGRLDAAAALALIRNARAAA"
     gene            complement(1091..1741)
                     /gene="hisH"
                     /locus_tag="VN968_19915"
     CDS             complement(1091..1741)
                     /gene="hisH"
                     /locus_tag="VN968_19915"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HXL31537.1"
                     /translation="MSVAIIDYGSGNLHSAAKAFERAARSMDVPEKIVVTRDPEVAFR
                     ADRIVLPGVGAFADCRRGLDALDGMIEAMTEAVRNKARPFFGICVGMQLMATRGKEHV
                     TTDGFNWIAGDVEKISPREENLKIPHMGWNTLDMIREHPVLERLPLGPKGRHGYFVHS
                     YHLNASNEADVLARADYGGPVTAMVGKDTAIGTQFHPEKSQRFGLALISNFLKWKP"
     gene            complement(1738..2268)
                     /locus_tag="VN968_19920"
     CDS             complement(1738..2268)
                     /locus_tag="VN968_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HXL31538.1"
                     /translation="MPVYTVHAPVMNGDGIAATDRFAFVRDGFHVWAALLAPIWLAWH
                     RLWLALIGWIVVVVALDVGMAALGVGSAAVFWVNLLIALLTGFEAASLWRWTLSWRNW
                     RQLDIVVADDEEAAERRFFDRWAAKQHALSNDQPAVDRGGPPPTRDIAGQPFSKPPPL
                     PGNSIIGLFPEPGASR"
     gene            complement(2295..2888)
                     /gene="hisB"
                     /locus_tag="VN968_19925"
     CDS             complement(2295..2888)
                     /gene="hisB"
                     /locus_tag="VN968_19925"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HXL31539.1"
                     /translation="MRKATIKRKTKETDIEVTVNLDGSGVSDVATGIGFFDHMLDLLA
                     RHSRIDMSVKAVGDLHIDHHHTTEDVGIALGQAVKQALGAMAGITRYASIHMPMDETL
                     SRVVIDISGRPILVFKVEFPRDKVGTFDTELVREWFNAFAINAGVTLHVETLYGENSH
                     HIAESCFKGLARALRAAVAIDPRAAGEVPSTKGQLGG"
     gene            3082..3642
                     /gene="hslV"
                     /locus_tag="VN968_19930"
     CDS             3082..3642
                     /gene="hslV"
                     /locus_tag="VN968_19930"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HXL31540.1"
                     /translation="MHELQSQSPVWHGTTILTVRKGGKVVIGGDGQVSIGQTVIKSNA
                     KKVRKLGKGDVIGGFAGATADAFTLFERLEGKLEQYPGQLTRAAVELAKDWRTDRYLR
                     RLEAMMIVADKDVSLVLTGTGDVLEPEAGVMAIGSGGNYALAAARALVDSDKDAETIV
                     RRSLDIAADICVYTNRNVTIETLQGG"
     gene            3646..4131
                     /locus_tag="VN968_19935"
     CDS             3646..4131
                     /locus_tag="VN968_19935"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL31541.1"
                     /translation="MAPDYLFRPMTSNDLPLVRRWLALPHVRQWWGDPEEQYSLVSGD
                     LDEPAMDQYIFSTAGNPFAYLQCYDLTAWNSGFGSHPPGTRGIDLFIGEPDMIERGHG
                     SALVRGFVDERLQHGAPRIVTDPDPANRRAVRAYEKAGFEKDRMVETSDGPALLMVRN
                     P"
     gene            4128..4376
                     /locus_tag="VN968_19940"
                     /pseudo
     CDS             4128..4376
                     /locus_tag="VN968_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009338610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 family protein"
     gene            4392..>4703
                     /locus_tag="VN968_19945"
     CDS             4392..>4703
                     /locus_tag="VN968_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXL31542.1"
                     /translation="MTDFSPREIVSELDRFIVGQTDAKRAVSIALRNRWRRLQLTGTL
                     REEVLPKNILMIGPTGVGKTEIARRLAKLAGAPFIKVEATKFTEVGYVGRDVEQIIRD
                     LV"
BASE COUNT          877 a         1538 c         1444 g          844 t
ORIGIN      
        1 atgtcgagaa agcacagctc gtcggcgccg gcggcgtcat aggcgatggc ggcttccacc
       61 ggatcgccgg cgtcgcgcag atcgacaaag ttgacgccct tgacgacccg gccgtccttg
      121 acgtcgagac aggggatcac gcggactttg aacatgcaaa actcctcgat catgatccga
      181 tcagattagg cggatcatga tctcattttc ttgtataaac gtgatctccg cgcaaacgcg
      241 tttcgcgttt gtcgcgaggg aaacccggtt gccgcttttc cggatcatgc gctaggccgc
      301 ggcccgcgcg ttacggatca gtgccagcgc cgccgcagca tcgagccggc cgtcatagag
      361 tgcgcggccc gcgatcgctc cggccagttt tttcgcacgc ggcgtcagca gcgccctcac
      421 atcctcgatg gaggcaaagc cacccgacgc gataacggga atcgagatgc gctctgcaat
      481 ctcgatcgtg gcatcgagat tgagaccttt cagcaacccg tcacgcgcaa tgtccgtaaa
      541 aatgatggcg gcgacgccgg catcctcgaa acgccgcgcg atttcgagcg cggtcacctc
      601 tgaggtctcg gcccagcctt cgaccgcgac cttgccgtcg cgcgcgtcga gacccaccgc
      661 aacccggccg gggaattttt tcgcggcacc cttcacaagg tcggggtcgc gtaccgccgc
      721 ggtgccgatg atgacgcgcg caacgccctt ggcgagccag gcctcgatgg tgttgagatc
      781 acggatgccg ccgccgagct gcagcggcat ggtgaccgcc ctgagcatcg cctcgacgac
      841 gtgcgcgttc atcggcttgc cggcaaaggc gccgtccaga tcgaccacgt gcagatattc
      901 aaacccctgc gcggcaaagc tgcgcgcctg cgccgtgggg tcgaggttga acacggtcgc
      961 gcgcgccatg tcgccctgtt caaggcgcac gcactggcca ttcttcaggt cgatggcggg
     1021 aaacaggatc atggaatgtt gcctgctccc tgcccccttg cgggggaggg tcggggaggg
     1081 aggtaccttg tcacggcttc catttcaaaa aattcgaaat cagagccagc ccaaagcgct
     1141 ggctcttttc cgggtgaaac tgcgtgccga tcgcggtgtc cttgccgacc atggccgtca
     1201 ccgggccgcc gtaatcggcg cgcgccagca cgtccgcctc gtttgacgcg ttgaggtgat
     1261 aggagtgcac gaaatagccg tggcggccct tcggccccag cggcagacgc tccagcaccg
     1321 gatgctcgcg gatcatgtcg agcgtgttcc agcccatatg gggaattttc aaattctcct
     1381 cgcgcggcga aatcttttcg acgtcgccgg cgatccagtt gaagccgtcg gtggtgacat
     1441 gctccttgcc gcgcgtcgcc attaattgca tgccgacgca gatgccgaag aacggccgcg
     1501 ccttgttccg cacggcctcg gtcatggcct cgatcatgcc gtcaagcgca tcgagacccc
     1561 ggcggcaatc ggcgaaggca ccgacgccgg gcagcacgat acgatcggcg cgaaacgcca
     1621 cttcgggatc gcgggtcacc acgatctttt ccgggacgtc catgctccgc gccgcgcgct
     1681 cgaacgcctt ggccgcggaa tgcagattgc cggagccgta atcgatgatg gcgacactca
     1741 ccgcgacgct cccggttccg gaaacaatcc gatgatgctg ttccccggca gcggcggcgg
     1801 cttcgaaaat ggctggccgg caatatcccg cgtcggcggc gggccgccgc gatcgaccgc
     1861 cggttgatcg ttgctcagtg cgtgctgctt ggcggcccag cggtcgaaaa accggcgctc
     1921 ggccgcctcc tcgtcgtctg ccaccacgat gtcgagctgg cgccagttgc gccacgacaa
     1981 ggtccagcgc caaaggctcg cggcttcaaa tccggtcagc agcgcgatca agaggttgac
     2041 ccaaaagacc gctgcgctgc cgacaccaag ggccgccatg ccgacatcga gtgcgaccac
     2101 cacgacaatc cagccgatca gcgccagcca cagccgatgc caggcgagcc agatcggggc
     2161 taatagcgcg gcccagacgt gaaagccgtc gcggacgaat gcgaaccggt cggtcgcagc
     2221 gataccatcg ccgttcatca cgggagcatg aactgtgtaa accggcatcg tacctctcgc
     2281 ccgcgagcag gaaatcagcc gccgagctga cccttggtgg atggcacctc gcccgcggcc
     2341 cgcggatcga tcgcgaccgc cgcgcgcagc gccctcgcca gacccttgaa gcaggattcg
     2401 gcgatatgat ggctgttctc gccatatagg gtctcgacgt gcaaagtcac gccggcattg
     2461 atcgcaaagg cgttgaacca ctcgcgaacc agctcggtgt cgaatgtacc gaccttgtcg
     2521 cgcgggaact cgaccttgaa caccaggatt ggccgtcccg aaatgtcgat cacgacccgc
     2581 gacagcgtct catccatcgg catgtggata ctggcatagc gggtgatgcc ggccatggcg
     2641 ccgagcgcct gtttgacggc ctgaccgagc gcgataccga catcctccgt ggtgtggtgg
     2701 tggtcgatgt gaaggtcgcc gaccgccttc accgacatgt cgatgcgcga atgccgggcc
     2761 agcaggtcga gcatatggtc gaaaaagccg atgccggtcg caacgtcgga cacgccggat
     2821 ccgtcgaggt tcaccgtcac ctcgatatcg gtctctttgg tcttgcgctt gatggtcgcc
     2881 ttgcgcatga aacccgcttt cccttgaccg actcttggcc gaagacgcgg cccttttaac
     2941 aggctgatgt cgccttcgcc actgcgggat ggcgccctgg aggccgaact tcggcctatg
     3001 gtgaccggaa tccgagggcc aaatcgcccc aattgcaacc gagcgcgcca atgcctaaat
     3061 ccctgccaat gcgaggtccc catgcacgaa ttacaatccc aatcgccggt ctggcacggc
     3121 acgaccattt tgaccgtccg caagggcggc aaggtggtga tcggcggtga cgggcaggtg
     3181 tcgatcggcc agaccgtcat caaatccaac gccaagaagg tccgcaaact cggcaagggc
     3241 gacgtcatcg gcgggtttgc cggcgccacg gcggacgcct tcacgctgtt cgagcggctg
     3301 gaaggcaagc ttgagcaata tccggggcaa ttgacccgcg ccgcggtcga gctcgccaaa
     3361 gactggcgta ccgaccgcta cctgcgccgg ctggaggcaa tgatgatcgt cgccgacaag
     3421 gacgtgtcgc tggtgctgac cggcaccggc gacgtgctgg agcccgaggc cggcgtgatg
     3481 gcgatcgggt ccggcggcaa ttatgcgtta gcggccgccc gcgctctggt cgattccgat
     3541 aaggatgccg agaccatcgt gcgccggtcg ctggatatcg ccgccgacat ttgcgtctat
     3601 accaatcgca atgtgaccat cgagaccttg cagggcggct gaccgatggc gccggactac
     3661 cttttccgcc cgatgacctc aaacgatctt ccattggtcc ggcgctggct cgccttgcct
     3721 catgtccggc aatggtgggg cgaccccgaa gaacagtata gcttggtcag tggtgacctc
     3781 gatgagccgg ccatggatca gtatatcttt tcaaccgccg gcaacccgtt cgcctacctc
     3841 cagtgctatg atctgaccgc atggaattca ggtttcgggt cgcatccgcc gggtacccgc
     3901 ggcatcgacc tgttcatcgg cgaacccgac atgatcgaac gaggccacgg ctcggcgctc
     3961 gttcgtggct tcgtcgatga gcgcctgcaa cacggcgcgc cgcgcatcgt cacggatccc
     4021 gatccagcca atcgtcgcgc ggtccgcgcc tatgagaaag cagggttcga aaaagaccgc
     4081 atggtcgaaa catcggatgg tccggccctg ctgatggtcc gcaacccatg acggccgagc
     4141 ccgacacggc ggccgattcc ggcctttcaa tctggcactg gtgggtgatc gcgggcggcc
     4201 tgatcgtcct gcaagcctcg atcctgtttg cgatgggccg gctgccgatc tgcgcctgcg
     4261 gttatgtgaa actctggcag ggcgtggtgc aaagcttgga gaattcgcag cacatcaccg
     4321 actggtacac cttccccgct catcatctga cgccgcttgt gccagcgcca tttcaaacct
     4381 agctaggacc tatgaccgac ttctcccccc gcgaaatcgt ttccgaactc gaccgcttca
     4441 tcgtcggcca gaccgacgcc aagcgcgcgg tgtccatcgc gctgcgcaac cgctggcggc
     4501 ggctgcagct cactggcacg ttgcgcgagg aagtgctgcc gaaaaatatc ctgatgatcg
     4561 gaccgaccgg cgtcggcaag accgagatcg cgcgacggct ggcgaaactt gccggcgcgc
     4621 cgttcatcaa ggtcgaggcc accaaattca ccgaggtcgg ctatgtcggc cgcgacgtcg
     4681 agcagatcat ccgcgatctc gtc
//
LOCUS       DATNOI010000496         6480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_627224,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000496 DATNOI010000000
VERSION     DATNOI010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627224"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VN968_19950"
     CDS             <1..347
                     /locus_tag="VN968_19950"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492775.1"
                     /note="One of the components of the high-affinity
                     ATP-driven potassium transport (or KDP) system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HXL31543.1"
                     /translation="LMTRGALTTFSIANDVAKYFAIIPAMFLAFYPQLAVLNVMHLAS
                     PQSAILSAIIFNALIIIALIPLALKGVAYRAIGAGALLQRNLMIYGLGGIIIPFIGIK
                     AIDLVVAALHLA"
     gene            359..964
                     /locus_tag="VN968_19955"
     CDS             359..964
                     /locus_tag="VN968_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HXL31544.1"
                     /translation="MLKEIRPAIILLVALTAITGLAYPLAMTAIAGVIFPKQAEGSLI
                     ERDGKVVGSALIGQEFKDDKYFHGRPSATSAPDPADSTKTVSAPYNAANSGGSNLGPT
                     SKALNDRVKEDVDKLKAENPSMPVPIDLVTTSASGLDPDISPEGALFQVPRVAKARNM
                     PEDRVRQLVTANTQGRLAGLLGEPRVNVLALNLALDAAGSK"
     gene            1006..3729
                     /locus_tag="VN968_19960"
     CDS             1006..3729
                     /locus_tag="VN968_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HXL31545.1"
                     /translation="MANQRRDLEKRPSPDALLEAARRENDQAGRLKIFVGAAPGVGKT
                     YEMLQSAHAKLKAGVDVVVGVVETHGRAETEALLQGLEVLPRKRLEYKDQILGEMDLD
                     AVIVRHPRIALVDELAHTNAPGSRHPKRYLDVEELLSHGIDVYTAVNIQHIESLNDVV
                     AQITHVRVRETVPDSVFDRADAIELIDLTPDDLIQRLKEGKVYVPKQAERALEHYFSP
                     GNLTALRELALRRTAERVDEQLLTHMQANAIAGPWAAGERILVCLSEDPRAAGLVRYT
                     KRLADRLHAPWTAVSIETRRSLQLTDEQRDRLADTLRLAEALGGEALTIPGVGRRIAD
                     DVISFAHANNVTQIIIGKSTRSWWFELMRGSVVHDLVRRAGNISVNVIAGEELAAEPV
                     PKKTVRAAERLEPFDPRPYLMSLLVVAAALGVAEVIQPHFGIENVDLVFLTAVVSVAV
                     RFGLWPSLLASVAASLCYNFFFLPPIYTFTITDPTNVAAFFFFMLIAMLVSNVAARVR
                     SQAVSAIGRVRTTESLYAFSRKLAGTATLDDVLWATAYQAALMLRVRVVLLLPEDGVL
                     TVKAGYPPEDELDKADLAAANWAWGNDRSAGRGADTLPGAKRLFLPMRTGRGLIGVIG
                     IDDDKTGPLLTPDQRRLLDALVDQGALAIERVLLVEDMDRVKRTVESDRLRSALLTSI
                     SHDLKTPLASVLGAASTMRDLASGLTDSEKRDLLATVIDESERLNRFIANLLDMTKLE
                     SGAIVPNTARHDIGEIVGSVLRRASKILAHHQVSLELAADLPMLELDAVLFEQVLFNL
                     LDNAAKYAPHDTTISIGSLRERDWVTLQVLDEGNGIPPTELESVFDKFYRVQKGDHVR
                     PGTGLGLAISRGFVEAMHGTISAANRTDRSGAVLTIRLPIPAASAALDTAA"
     gene            3726..4415
                     /locus_tag="VN968_19965"
     CDS             3726..4415
                     /locus_tag="VN968_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL31546.1"
                     /translation="MSAAPIKVLVIDDEPPIRKLLRMGLSTQGYEILEASNGKVALEL
                     LAQEPALIILDLGLPDIQGHELLRMIRGRNDSVPIVVLSSRGDEAGKVQALDLGADDY
                     LTKPFGMDELLARMRAALRHQLQVHGERPLFRSGDLNVDLVRRIVKVGDKDVKLSPKE
                     YELLRVLVQHAGKVLTHRFLLKELWDELTDAQYLRVYVRQLRQKIEADPERPQFVLTE
                     TGIGYRLRAPD"
     gene            4636..5076
                     /locus_tag="VN968_19970"
     CDS             4636..5076
                     /locus_tag="VN968_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HXL31547.1"
                     /translation="MKISDLLSPTDVMIDVRASNKPLLLQEFAAKAADSLGLPVDQIA
                     PYLLKREELGSTGIGRGVAIPHARLPDLQRPHGLLAKLKQPIEFDAIDGQAVDIVFVL
                     LLPAAVENAQLSALALVARTLRPPENLARLRSAKNSSELYSAIV"
     gene            complement(5086..6102)
                     /locus_tag="VN968_19975"
     CDS             complement(5086..6102)
                     /locus_tag="VN968_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HXL31548.1"
                     /translation="MNVASSAVLATEPANLEGVVAAGAYVDGRRVANIAIDEASSWRS
                     RPGHVVWIGLHEPDIALLTSVQQQFQLHDLAIEDADHAHQRPKIEQYGDGLFIVARTA
                     QLVGDSIAFGETHLFVGEGYLVSVRHGASTSYAPVRERCESCPRALARGEDYILYAIL
                     DFIVDNYSPVLETIQEEVEAMEALVLASAMTRAQIERLYLLRRDLLRLRNAVGPLVEV
                     CRRLEHDNLPMVRPTMQPLFRDVTDHVRTVQEQIDSLREVLAFAFEASLLVGQAQETA
                     VSKKLASWLAIIAVPTAVAGIYGMNFKNIPELQLEYGYFIVMGVMAIACAALFWRFRR
                     VGWL"
     gene            6300..>6480
                     /locus_tag="VN968_19980"
     CDS             6300..>6480
                     /locus_tag="VN968_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HXL31549.1"
                     /translation="MARKYSKKVSAKVGRAMKKRKAGTLKSGRSGKKVTSRKQAIAIA
                     LSEARAEGKKVPKKAA"
BASE COUNT         1248 a         1992 c         1991 g         1249 t
ORIGIN      
        1 tgttgatgac ccgtggcgcg ctcaccacgt tctcgatcgc caacgacgtc gcgaaatatt
       61 tcgccatcat ccccgcgatg ttcctggcgt tctatccgca actcgccgtg ctcaacgtga
      121 tgcatctggc gagcccgcaa agcgccatcc tgtcggcgat catcttcaat gccttgatca
      181 tcatcgcgtt gattccgctg gccttgaagg gcgtcgctta tcgggcgatt ggagcaggcg
      241 ctctattgca gcgtaacctg atgatctatg gcctcggcgg catcatcatc ccgttcatcg
      301 gcatcaaggc catcgacctc gtcgtcgccg ccttgcatct ggcctgagga gagacaccat
      361 gttgaaggaa attcgccccg ccatcatcct gctggtcgcg ctcacggcga tcaccggttt
      421 ggcttatccc ctagccatga ccgcgatcgc aggcgtgatc tttccgaagc aggcggaagg
      481 aagcctgatc gaaagggatg gcaaggtggt gggctccgcc ctgatcgggc aggaattcaa
      541 ggacgacaaa tatttccatg gccggccctc ggcgacctcg gcgccggacc ccgccgattc
      601 caccaagacc gtttcggcgc cctataatgc ggccaattcg ggcggatcca acctcggccc
      661 gaccagcaag gccctgaatg atcgggtcaa ggaagacgtc gacaagttga aggccgagaa
      721 cccctcgatg cctgttccga tcgatctcgt caccacgtcg gcgagcggcc tcgatcccga
      781 tatctcgccg gagggcgcgt tattccaggt gccgcgtgtt gccaaggcgc ggaatatgcc
      841 ggaagatcgg gttcgccaac tggtcacggc gaatacgcag ggtcgcctcg ccgggttgct
      901 cggcgaaccg cgcgtcaacg tattggcgct caatctggcg ctggacgccg ctggatcgaa
      961 atagagccat ctaggcggta tcaggcgtga ggactatatt gccgtatggc aaatcaacgc
     1021 cgcgaccttg aaaaaagacc ctcgccggac gcgcttctcg aagccgcccg gcgagagaac
     1081 gatcaggccg gccggcttaa aatcttcgtc ggcgcagcac cgggcgtcgg caagacctac
     1141 gaaatgctgc agagcgccca cgccaaactg aaggcgggcg tcgacgtcgt cgtcggcgtg
     1201 gtcgagaccc atggacgggc ggagaccgaa gcgctgttgc aaggccttga ggtcctcccg
     1261 cgcaaacggc tcgaatacaa ggaccagatt ctcggggaga tggatctcga tgctgtcatc
     1321 gtgcgccatc cgcggatcgc gctggtcgat gaacttgccc acaccaacgc gcccggcagc
     1381 cgccatccga agcgctatct cgacgttgag gaactgctct cgcacggcat cgacgtctac
     1441 accgcggtca atattcagca catcgagagc ctcaacgacg tggtcgcgca gatcacgcat
     1501 gtacgggtcc gcgagacggt gccggattcg gtgttcgatc gcgcggatgc gatcgagctg
     1561 atcgatctga cgccggacga tctgattcag cggttgaaag agggcaaggt ctatgtccca
     1621 aagcaggccg agcgcgcgct tgagcattac ttttcgccgg gtaatctgac cgcgctccgc
     1681 gaactggcgc tgcggcggac cgccgagcgc gtcgatgaac aactcctgac tcatatgcag
     1741 gccaacgcca ttgccggccc ctgggccgcg ggcgagcgca ttctggtttg cctcagcgag
     1801 gacccgcgcg cggcggggct cgtgcgttac accaagcggc tggcggaccg gctgcacgcg
     1861 ccctggaccg ctgtcagcat cgagacaagg cgcagcctgc aattgaccga tgagcaacgc
     1921 gaccggctag ccgacacgct gcggctggcc gaggcgctgg gcggcgaggc gctcaccatt
     1981 cccggcgtcg gacgccgcat tgccgatgac gtgatcagct tcgcgcacgc caacaacgtg
     2041 acccagatca tcatcggcaa gtcgacgcgt tcctggtggt tcgagctcat gcgcggctcg
     2101 gtggtgcacg atctggtgcg gcgcgccggc aatatcagcg tcaacgtcat cgccggcgag
     2161 gaactcgcgg cggagccggt gccaaagaag accgtgcgcg cggccgaacg actggagcct
     2221 ttcgatccgc ggccctatct gatgtcgctt ttggttgtcg cggccgcgct cggggtcgcc
     2281 gaagtgatcc agcctcattt tggaatcgag aacgtagacc tggtatttct cacggcggtg
     2341 gtcagcgtgg cggttcgctt cggtctttgg ccgtcgctgc ttgccagcgt cgcggcgtcg
     2401 ttatgctaca actttttctt cctgccgccg atttacactt tcacgatcac cgatccgacc
     2461 aacgtcgcgg cgttcttctt cttcatgctg attgcgatgt tggtctccaa tgtcgcggca
     2521 cgcgtgcgct cccaggccgt gtccgcgatt ggcagggtgc ggaccacgga atcgctttat
     2581 gccttcagcc gcaagcttgc gggcacagcg acgctggacg atgtattgtg ggcgaccgcc
     2641 tatcaggcgg cgctgatgct cagggtacgg gtggtgttgc tgctgcccga ggacggcgtc
     2701 ctcacggtga aggcgggcta tccccctgaa gatgaactgg acaaggccga tcttgccgcc
     2761 gcgaactggg cctggggcaa cgaccgctca gccgggcgcg gcgcggatac gctgccgggc
     2821 gccaagcgcc ttttcctccc gatgcgaacc ggacgcgggc taatcggcgt gatcggtatc
     2881 gacgacgaca agaccggccc actcttgaca ccggaccagc gccgtctgct cgatgcgctg
     2941 gtggatcagg gcgcgctggc gatcgaacgg gtgctgctgg tcgaggatat ggaccgggtc
     3001 aaacgtactg tggagtcgga ccgtttgcgc tcggcgctgc tgacctcgat ctcgcacgat
     3061 ctcaagacgc cgttggcctc ggttcttggc gccgccagca cgatgcggga tctcgccagc
     3121 ggcctgactg actcggaaaa gcgcgatctg ctcgccaccg tgatcgacga atccgagcgg
     3181 ctcaaccggt tcatcgccaa tctgctcgac atgaccaagc tggaatccgg cgccatcgtg
     3241 ccgaatacgg cgcggcacga tatcggcgag atcgtcggca gcgtgttgcg gcgcgccagc
     3301 aagatcctcg cccatcacca ggtctcgctt gaactcgccg ccgatttgcc gatgctggag
     3361 cttgacgcgg tattgttcga gcaggtgctc tttaacctcc tcgacaacgc ggcgaaatac
     3421 gcacctcacg acaccacgat atcgatcggg agtttgcggg aaagggattg ggtcaccttg
     3481 caagtcctcg acgagggcaa cggcattccg ccgacggaac tggagagtgt atttgacaaa
     3541 ttctatcgcg tgcaaaaggg cgaccatgtc cgccccggca ccggcctggg gctcgcgatt
     3601 tcgcgcggct ttgtcgaggc catgcatggc acgatttccg ccgcgaatcg caccgatcga
     3661 agcggcgccg tcttgaccat ccgattgccg atcccggctg catcagccgc gctggacacc
     3721 gccgcatgag cgctgccccg atcaaggtcc tcgtcatcga cgatgagccg ccgatccgca
     3781 aattgttgcg gatgggattg agcacgcagg gctacgagat cctggaggcc tcgaatggca
     3841 aggtggccct cgaattgctg gcccaggaac ccgcactgat cattctcgat ctcggcttgc
     3901 cggatatcca gggccatgaa ttgctgcgca tgatccgcgg ccgcaacgac agcgttccga
     3961 tcgtggtgct ctcaagccgt ggcgacgagg ccggcaaagt ccaggcactc gatttgggcg
     4021 ccgacgatta tctgacaaaa ccgttcggga tggatgagct gctcgcgcgc atgcgcgcgg
     4081 ccttgcggca tcagttgcag gtccatggcg aacgcccgct gtttcgatcg ggcgatctaa
     4141 acgtcgatct ggtgcgccgc atcgtcaagg tcggcgacaa ggacgtcaag ctttcaccga
     4201 aggagtacga attgttgcgc gtgctggtgc agcacgccgg caaggtgttg acccatcggt
     4261 ttctgctcaa ggagctgtgg gatgaactga ccgacgcgca atatttgcgg gtctatgtgc
     4321 gccagctcag gcagaagatc gaagccgatc cggagcgccc gcaatttgtc ctgaccgaaa
     4381 ccgggatcgg ctaccgactg agggcgccgg attaatgccc ggcagttggc tttagctttt
     4441 tgatctcttc ccgttgtcgg gagggaggcg taaaccgccc ctctagatca tgatccgatt
     4501 agatggaatc gaatcatgat ctcatctctt tgtttgagca tgatcttttc ggaaaaccgg
     4561 cttccacttt tccggatcat gctctagacc gagacctaat caatgctttt aacgatgcgc
     4621 cctcctggag cgaccatgaa aatctccgat ctgctgtcgc caacggacgt catgatcgac
     4681 gttcgagcct ccaacaagcc gctgctgttg caggaattcg cggccaaagc cgctgatagt
     4741 ctcggcctgc ctgtcgatca gatcgcgccc taccttctca agcgggaaga actcggatcg
     4801 accgggatag gccggggcgt cgccattccg catgcccggc tgcccgattt gcagcgacct
     4861 cacgggctgc ttgcgaaatt gaagcagccg atcgaattcg acgccatcga tggtcaggcg
     4921 gttgatatcg tgtttgttct cttgttgccg gctgccgtgg agaatgcaca gctcagcgcg
     4981 ctcgcgttgg tggcccggac gttaaggccg ccggaaaatc tcgcgcgatt gcggagcgca
     5041 aagaattcat ccgagcttta ttcggcgatc gtctgacgat cgggctcaca gccatccgac
     5101 gcgccggaat cgccagaaca gcgcagcgca cgcaatcgcc atcacgccca taacgatgaa
     5161 ataaccatat tccaactgca gttccgggat gttcttgaag ttcatgccgt agattccggc
     5221 gaccgcggtc ggaacggcga tgatggcaag ccatgaggcc agttttttgg agacggcggt
     5281 ctcctgagcc tgaccgacca gaaggctcgc ttcgaaggca aatgcaagaa cctctcgaag
     5341 cgagtcgatc tgctcctgaa ctgtcctgac gtggtccgtg acgtcgcgga acagcggctg
     5401 catggtcggt cgcaccatcg gcaggttgtc atgctcaagc ctgcggcaaa cctccaccag
     5461 cggcccgacc gcgtttcgca ggcgcaacag gtcgcgtcgc agcagataaa gccgctcgat
     5521 ctgcgcccgt gtcatcgcgc tcgccagcac cagcgcctcc atggcctcga cttcctcctg
     5581 gatggtttcg agcaccggcg aatagttgtc gacgatgaaa tcgaggatgg catagaggat
     5641 atagtcctcg ccgcgggcca gcgcccgtgg gcagctctcg cagcgctcgc gtaccggcgc
     5701 ataggacgtc gacgcgccat gacggaccga aacaagatat ccttcgccga cgaacagatg
     5761 ggtctcgcca aatgcgatgc tgtcgccaac gagttgagcg gtgcgcgcca cgatgaacag
     5821 cccgtcgcca tattgctcga tcttcggtcg ctggtgggcg tggtccgcat cctcgatggc
     5881 gagatcgtgc agctggaatt gctgctgcac gctggtcaag agcgcgatat caggttcgtg
     5941 cagcccgatc cacaccacat ggcccggccg gctgcgccag cttgaagctt cgtctatggc
     6001 aatattggcg acgcgtcggc catcgacata ggcgccggct gcgacgactc cttcgaggtt
     6061 ggcgggctcc gtcgccaaga cggctgatga cgctacgttc atgatttccg tccccagtga
     6121 ccgctgttga actttggctc cgaggcaaag ttctcagggg aaggctagca ctggagaacc
     6181 cgccgtcaaa tggaatgtcg gaacgccgct gacgatgaat cacggatctt gcttcgggcg
     6241 cgccttcggg aaccatctcc atccgtgcag attattttcc cgcaattggg agaatggtca
     6301 tggcacggaa gtattcgaaa aaggtatccg ccaaagtcgg gcgcgcgatg aagaagcgca
     6361 aggccggcac tctgaagagc ggccgctccg gcaagaaggt cacaagccgc aagcaggcga
     6421 tcgcgatcgc tctttccgag gcgagggccg agggcaagaa agtcccgaag aaggccgcga
//
LOCUS       DATNOI010000497         7476 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2517391,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000497 DATNOI010000000
VERSION     DATNOI010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7476
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2517391"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VN968_19985"
     CDS             <1..476
                     /locus_tag="VN968_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AprI/Inh family metalloprotease inhibitor"
                     /protein_id="HXL31550.1"
                     /translation="PFTKDITAWNIRGLDIVRLQDAAGQSVIDFTEVESGIFEGLRTN
                     EGIYILQNLAAARSLAKSMDQMIGDWSMVRGNGRPVCGLTLTNTEATPDNFAVFLKPR
                     CDPVVTAFAPTVWRLERGELLLMSQNGETWRFEADDNAQWRRVPDSADPLIMLRQ"
     gene            632..1003
                     /locus_tag="VN968_19990"
     CDS             632..1003
                     /locus_tag="VN968_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003563531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXL31551.1"
                     /translation="MRFMSIVTSPQPPGPPPPEMMEAMGKLADREIKAGRMVDTGGLM
                     PLATGAQVRITDGRLSVIDGPFVEAKEVIGGYAIFELRNKEEAVALAVEFMQLHKDLM
                     PGWEGTCEVRAFFTDAPGECS"
     gene            1080..1430
                     /locus_tag="VN968_19995"
     CDS             1080..1430
                     /locus_tag="VN968_19995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXL31552.1"
                     /translation="MRFMYIVTPAHHVPPTTELMEAVHKLAEREIKAGHMLDSGALMP
                     RTLGAQVRITDGRLSVIDGPFVEAKEVIGGYAIFELRDKEEAVAKAVEFMQVHRDHMP
                     GWEGTCEVRAFAGP"
     gene            1462..2721
                     /locus_tag="VN968_20000"
     CDS             1462..2721
                     /locus_tag="VN968_20000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003563530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXL31553.1"
                     /translation="MTAADIHRAIVAVWRIEQPRLITSLSRMLRDVPLAEDLTQEALL
                     AAVEHWPVTGVPAKPGAWLMATAKRRALDQLRRRRMLARKHEIVARDMEQDQQAMPDW
                     DAALDDDIGDELLRLIFTACHPLLSREARAALALRMICGLTTEEIARAFLLPDATIAQ
                     RIVRAKRTLSESGLAYETPRRGELSERLSSVLEVVYLIFNEGYTAARGEHWLRPQLCH
                     EALRIGRVLTSIAPLEPEAHGLLALMELNASRTAARTDASGEPILMLEQNRALWDQLQ
                     IRRGMQALGRARELEGAGGFYALQAAIIACHAQASTADETDWPRISGLYAELAALVRS
                     PIIELNRAVAVGMAEGPEAALVIVDSLAHEPALKTYHLLGSVRGDLLHRLGRHAEARA
                     AFEAAAALAGNKREQDLLRRRAAEPPT"
     gene            complement(2749..5070)
                     /locus_tag="VN968_20005"
     CDS             complement(2749..5070)
                     /locus_tag="VN968_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXL31554.1"
                     /translation="MAHQRTLTLAHWGVYEVEYDEAGKAIHLHSFSKDPDPSPIGLHM
                     LSDEVARLRVRRPAVRKSWLEHGPGAFPERRGAEPFVEVSWNEALDLVAGELGRVKRD
                     FGNQAIFGGSYGWASAGRFHHAQSQLRRFLNSIGGFVPHKDDYSIGAALVLMPHIVAP
                     LEEILASHTSWEMLAKHCKLFVSFGGVPRKNAQINAGGAIVHNVKGGLFRMRDAGVRF
                     VNVTPTADDLDTGGDIEWLSIRPNTDAAMILALCHTLLVEGLHDQAFLDRYTVGFDKF
                     CPYLSGDQDGQPKDAAWAEKITGIAADRIVALAREMAATRTMVSIGWSLQRSHHGEQP
                     FWALVTLAAMLGQIGLPGGGFGCGYGPANLMGSGALRHPGPTLPQGTNPVSDFIPVAR
                     IADMMLQPGERFSYNGAEHRYPDIRLVYWAGGNPFHHHQDLNRLRLAWRKPETVVFHE
                     QFWTPAAKMSDIVLPATTGLERDDIGYARREPYLIAMKKAREPIGEARDDYAIFQAIA
                     QRMGADRAQSDGREISEWLSHLYEEARVKSAKVGVGLPPFEEFWEAGIAEAVGTQQPP
                     LMLGPFRADPLAHPLRTPSGKIEIYSERIASFGYDDCPPHAVWLEPIEWLGGKTAERY
                     PLHMLSDQPADKLHSQLDHGPHSRATKVKSRQPITLHPEDAAARGVTEGDLVRVFNDR
                     GACLAAARLSDRIRRGVVRLSTGAWFDPADSGANRPLEKHGNPNALTLDIGASKLSQG
                     CSAQTCLVEIERLEGPAPAVTAHDLPSFVKRGA"
     gene            complement(5332..5832)
                     /locus_tag="VN968_20010"
     CDS             complement(5332..5832)
                     /locus_tag="VN968_20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31555.1"
                     /translation="MKVIFWARGLALFSLLAFGSALAADGGHNPLAEHVRDANSRFKD
                     VKVAVAEGYAPIPCTSGIDGGAMGVHYVNGEYLKDEVPDIKRPQAVMYEPMPDGKMEL
                     VAVEYITSKGPASLEGQLFSFTGAPNRYGLGPFYELHVWAWKANPRGAFADMNPNVTC
                     EHAHGK"
     gene            6028..>7476
                     /locus_tag="VN968_20015"
     CDS             6028..>7476
                     /locus_tag="VN968_20015"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL31556.1"
                     /translation="MLRVSVLGNLAVSRHGMRMQLPPSKKTRALLAYLAVTDRPHSRD
                     RLCAMFWPVPDDPRAALRWSLTRLRPLIDEPDCRRIVADRESAGLDLDRDTVDILSLR
                     DLVRNGTDAVLTDTLRQATEALEVDFLEGLDLPDCHEFQSWCIGEREETRRLRVRLLT
                     ALVNRLADVPDEALRHARALSLLAPADEGAQAMLVRLLRAAGYSREAEQQFQRAERRL
                     EEFDAVRTGALRQAAQRSFQAKLPPRVDDQIVHRPHAGRLRARPALHEVQFCRTSDGV
                     RIAYARVGDGPPLVWAAHWLSHLAFSWESPIWRHWTEEFAKDHSFVHYDERGNGLSDW
                     DNPTFSVDAFVRDLEAVVDALGFDRFALIGSSKGGATAMAYAARHPERVSHLILCGAY
                     AQGWRVWGNAAEIERREAIITLTRQGWAQDNPAFRQILTSLLLPDATVEEMGSFNDLQ
                     RISASAENAARLLQSSGDVNVLDLLPGIVAPTL"
BASE COUNT         1269 a         2371 c         2505 g         1331 t
ORIGIN      
        1 tgccgttcac caaggatatc acggcctgga atatcagggg actggatatc gtgcggctgc
       61 aggatgccgc cggacaatcg gtaatcgact tcaccgaggt cgagagcggg attttcgaag
      121 gcctgcgcac caacgagggc atttatattt tgcagaacct cgccgccgcc cgctcgctcg
      181 ccaaatcgat ggatcagatg atcggcgact ggtcgatggt ccgcggcaac ggccgcccgg
      241 tctgcggcct gaccttgacc aacaccgagg cgacgccgga caatttcgcg gtgttcctca
      301 agccgcggtg cgatcccgtg gtcacagcgt ttgcgccgac agtgtggcgg ctggagcgcg
      361 gcgagttgtt gctgatgtcg caaaacggcg agacctggcg cttcgaagcc gacgacaacg
      421 cgcagtggcg gcgggtgccc gacagcgccg atccgctgat catgctgcgg cagtagaaat
      481 tcgtcatttc cagatgtgcg attgcacatc atgacggcaa attttttcaa aaagcatgtc
      541 gatccgccgg gggctggttc gtcgtctcaa ttagcgggac agttccgcgg ggcagggtgc
      601 ccgcggctcg cctcaaaaag ggagtttttg catgcgcttc atgtcgatcg tcacctcgcc
      661 ccagcctccc ggtcccccgc ccccggaaat gatggaggcg atgggcaagc tcgccgaccg
      721 ggaaatcaag gccggtcgca tggtcgacac cggcgggctg atgccgctcg ccacgggcgc
      781 gcaggtgcgc atcaccgacg gccggctcag cgtgatcgac ggcccgttcg tcgaggccaa
      841 ggaggtgatc ggcggttacg cgatcttcga gctgcggaac aaggaggagg ccgtggcgct
      901 ggcggttgaa ttcatgcagc tccacaagga cctcatgccg ggctgggaag ggacctgcga
      961 ggttcgcgca ttttttaccg acgctccggg cgagtgttct taacagaggc cggtggtcat
     1021 gacggctgcc ggcatcgatg gcgatggcgg cggcttaccc ttatcaagga gttctcgata
     1081 tgcgctttat gtacatcgtc acgcctgccc accacgtgcc tccgaccacc gaactcatgg
     1141 aggccgtgca caagcttgcg gagcgcgaga tcaaggccgg ccacatgctc gatagcggcg
     1201 cgctgatgcc gcgcacattg ggcgcgcagg tgcgtatcac cgacggccgg ctcagcgtga
     1261 tcgacggtcc gttcgtggag gccaaggagg tgatcggcgg ttacgcgatc ttcgagctgc
     1321 gggacaagga agaggccgtc gcgaaggcgg tcgagttcat gcaggttcac agggatcaca
     1381 tgccgggctg ggaaggaacg tgcgaggttc gcgcgtttgc cgggccttga ggcaagggcc
     1441 cgccaaacgc gattggtcgc catgacggcc gccgacatcc atcgcgccat cgttgcggtc
     1501 tggcggatcg agcagccccg gctgatcacg agcctttcaa ggatgctgcg cgatgtgccg
     1561 ctggcggagg atctgacgca ggaagcgctg ctggcggcgg tcgagcattg gcccgtcacg
     1621 ggcgtcccgg cgaaacccgg cgcatggctg atggcgaccg ccaagcgccg ggcgctggat
     1681 cagttgcgcc ggcgccggat gctcgcgcgc aagcacgaga tcgttgccag ggacatggag
     1741 caggatcagc aggccatgcc cgattgggac gcagcactcg acgacgacat cggcgacgag
     1801 ctgctgcggc tgatcttcac cgcctgtcat ccgctgttgt cgcgcgaggc ccgcgcggcg
     1861 ctggcgctgc ggatgatctg cggcctgacc accgaggaga tcgcgcgagc cttcctgctg
     1921 ccggacgcga cgatcgccca gcgcatcgtg cgcgccaagc ggacgctttc ggaatcgggg
     1981 ctggcctacg agaccccgcg ccgcggcgaa ctctcggagc gtctttcctc ggtgctggag
     2041 gtcgtctatc tcattttcaa cgagggctac accgcggcgc gcggcgaaca ctggctgcgt
     2101 ccccagctct gccatgaagc gcttcggatc ggccgcgtgc ttacctcgat cgcgccgctt
     2161 gagccggaag cccatggatt gctcgccctg atggagctga acgcctcgcg cacggcggcg
     2221 cgtactgacg cctccggcga gccgatcctc atgctggagc agaaccgcgc cctgtgggac
     2281 cagctccaga tccggcgagg catgcaggcg ctcgggcgcg cgcgcgaact ggagggggca
     2341 ggcgggttct acgccctgca agcggccatc atcgcctgcc acgcgcaggc gtcgacagcg
     2401 gatgaaaccg actggccgcg catatcagga ctttatgcgg aattggcggc gctggtgcgc
     2461 tcgccgatca tcgaactcaa tcgcgcggtt gccgtcggca tggccgaggg cccggaagcg
     2521 gcgctcgtca tcgtggacag ccttgcgcat gagcccgcgc tcaaaaccta tcatctatta
     2581 ggaagcgttc gcggcgacct gctgcacaga ctcggccgcc acgcggaagc ccgcgccgcg
     2641 ttcgaagcgg cggcagcact tgcgggcaac aagcgtgagc aggacctgct gcgacgccgc
     2701 gccgccgaac ctccgacctg agagcgagcg gtccgtagct aagagcggtt atgcccctcg
     2761 cttcacgaag ctaggcaaat cgtgcgcggt cacggctggc gctgggcctt ccaggcgctc
     2821 gatctcgacc aggcaggtct gggcgctgca gccctggctc aacttgctgg cgccgatatc
     2881 aagggtgagc gcattgggat tgccgtgctt ctccagcggc cggttggcgc ctgagtcggc
     2941 cgggtcgaac caggcgccgg tgctgagccg tacgacgccg cggcggatgc ggtcgctcag
     3001 gcgcgcggcg gcgaggcagg cgccgcggtc gttgaaaacg cgcaccaagt cgccctcggt
     3061 gacgccgcgg gccgcggcgt cctcgggatg tagcgtgatc ggctgacggc ttttgacctt
     3121 ggtcgcgcgg gaatgtgggc cgtgatcgag ctgactgtgc aatttgtcgg ccggctggtc
     3181 cgacagcatg tgcagcggat agcgctcggc agtcttgccg cccagccact cgatcggttc
     3241 cagccagaca gcgtgcggtg ggcagtcgtc gtaaccgaac gaggcaatgc gctcggaata
     3301 gatctcgatc ttgcccgatg gtgtcctgag tggatgtgcc aggggatcgg cgcggaacgg
     3361 tccgagcatc agcggcggct gctgtgtgcc cacggcctcg gcgataccag cttcccagaa
     3421 ttcttcgaac ggcggcaggc cgacgccaac cttggccgat ttgacgcgcg cctcctcgta
     3481 gaggtgagag agccattcgc tgatctcgcg gccgtcggac tgggcacggt cggcgcccat
     3541 gcgctgggcg atcgcctgga agatcgcata gtcgtcgcgc gcttcgccga tcggctcgcg
     3601 tgctttcttc atagcgatca ggtagggctc gcgcctggcg taaccgatat cgtcacgctc
     3661 caggccggtg gtagcgggta gcacgatatc ggacatcttg gcggccggcg tccagaactg
     3721 ctcgtggaac accaccgtct cgggcttgcg ccaggccagc cgcagccggt tgaggtcctg
     3781 gtggtgatgg aacggattgc cgcccgccca atagaccaga cggatgtcgg gatagcggtg
     3841 ctcggcgcca ttgtaggaga agcgctcccc gggctgcagc atcatgtcgg cgatgcgcgc
     3901 caccggaatg aagtcgctga ccgggttggt gccctgcggc agggtcgggc cgggatggcg
     3961 cagcgcgccg ctacccatca gattcgccgg cccgtagccg cagccgaagc caccgccggg
     4021 cagaccgatc tggcccagca tggcggccag cgtgaccagc gcccagaaag gttgctcgcc
     4081 gtggtgcgag cgctgcagcg accagccaat gctgaccatg gtgcgggtcg ccgccatctc
     4141 gcgtgccagc gccacgatgc ggtcggcggc aatgccggtg atcttttcag cccaggccgc
     4201 gtccttgggc tggccgtcct ggtcgccgct gagatagggg cagaacttgt cgaagcccac
     4261 cgtgtagcgg tcgaggaagg cttgatcatg gaggccctcg accagcagcg tgtggcaaag
     4321 cgccaggatc atcgcggcgt cggtgttggg ccggatcgac agccactcga tatcaccgcc
     4381 agtatcaaga tcatcggccg tcggcgtcac attgacgaag cgcacacccg cgtcgcgcat
     4441 tctgaagagg ccgcctttga cattgtgcac gatggcgccg ccggcgttga tctgggcgtt
     4501 cttgcgcggc acgccgccga aggacacgaa aagcttgcag tgcttggcga gcatctccca
     4561 gctcgtgtgg ctcgccaata tctcctccaa cggggcgaca atatgcggca tcagtacgag
     4621 ggcagcacca atgctgtagt catccttgtg aggcacgaag ccgccgatcg aattgaggaa
     4681 gcgccgtagc tggctttggg cgtgatgaaa acgcccggca ctcgcccagc cataggagcc
     4741 gccgaagatc gcctggttgc cgaaatcgcg cttgacgcga ccgagttcgc cagccaccag
     4801 gtcgagcgct tcattccacg acacttcgac gaagggctcg gcgccgcggc gctccgggaa
     4861 ggcgccggga ccatgctcga gccagctctt gcgcacggcg ggacggcgta cgcgcaggcg
     4921 cgccacttcg tcggacagca tgtgcagccc gatcggcgag ggatcgggat ccttcgagaa
     4981 ggaatgcaga tggatggcct tgccggcctc gtcgtattcg acttcgtaga cgccccaatg
     5041 ggccagggtg agcgtgcgtt gatgagccat ggaacggtca gcctcgcggg cgatggaaaa
     5101 atagcgtcgc tgcatcagcg gtcgctgaaa gaaggaacgc agcgcacctg aaaacgtctg
     5161 gcttgctcta ccgaaatctc gtgctttcgg tgcgggatac ggccgcccgc gccgcgcttc
     5221 ctgctcggcc agagcctgtt gtctcgacgt gaccccgcgc aggcgagcga cccttctcgt
     5281 cgcctgcgcg ccaacactcc atcgcagagc tagccgagag cgaggcgcgt gttacttccc
     5341 gtgcgcgtgc tcgcaggtca cgttgggatt catgtccgcg aacgcaccgc gcgggttcgc
     5401 tttccaggcc cacacgtgca attcgtagaa cgggccgagg ccgtagcgat taggcgcgcc
     5461 ggtgaagctg aacagttggc cttccagcga ggccggtccc ttggaggtga tgtattcgac
     5521 cgcgacgagc tccatctttc catcgggcat cggctcatac atcaccgctt gcggccgctt
     5581 gatatcgggt acctcgtcct tcaggtattc gccgttgacg taatgcacgc ccatcgcgcc
     5641 gccatcgatg ccgctcgtgc aagggatcgg cgcgtagcct tcggcgaccg cgaccttcac
     5701 gtccttgaag cggctattgg cgtcgcgcac atgctccgcc aggggattgt gaccgccatc
     5761 ggcggccaat gcactgccaa acgcgagcaa tgaaaagaga gccagacctc tggcccaaaa
     5821 tattactttc atcctgttct ccttgatgcg gcggcccgat gccgccgctg ccggtctctt
     5881 caatgccgcg ttccttgcgg gtcgatcgga ggaaaagccg ttgttgtcag cgtggaaaaa
     5941 tcggatactt gcgttggaat ttgcgggcct gccggtggcc gcctgcgcgc aggagcccaa
     6001 ggcgcccgga tcatgaaagg gaaagagttg ctgcgcgttt ccgtgctggg caatcttgcc
     6061 gtgtcgcgcc acggcatgag aatgcagctg ccgccgtcca aaaagacgcg ggcgttgctc
     6121 gcctatctgg cggtcactga ccgcccgcac agccgcgacc ggctctgcgc catgttctgg
     6181 cccgttccgg atgatccccg tgccgcgctg cgctggagtc tgacccgatt gcggccgctc
     6241 atcgacgagc cggattgtcg aaggatcgtc gccgatcgcg aaagcgccgg gctcgatctt
     6301 gatcgtgaca ccgtggatat cttgtcgttg cgcgatctgg tccgaaacgg caccgatgcg
     6361 gtcctgaccg atacgctgcg ccaggcgacc gaagcgctcg aggtcgattt cctcgagggt
     6421 ctcgatctgc cggactgcca cgagtttcaa agctggtgca tcggggaacg tgaggagacg
     6481 cggcggcttc gcgtgcgact gctgaccgcg ctggtaaacc gtctcgcgga cgtgcccgat
     6541 gaagcgttgc gccacgctcg cgcgctaagt ctcctcgcgc ctgccgatga gggcgcccag
     6601 gcgatgctgg ttcgattact gcgcgctgcc ggctattcgc gtgaggccga gcaacagttt
     6661 cagcgcgctg aaaggcggct cgaagagttc gacgcagtgc gcaccggcgc cctgcggcag
     6721 gcggcgcaac ggtcgtttca ggccaaattg ccgccgcgcg tggacgatca aatcgtccat
     6781 cgaccccacg ccgggcgctt gcgtgcacgc cccgccttgc acgaggtgca gttttgcagg
     6841 acttcggatg gtgtccggat cgcgtatgcc cgcgtcggcg acggcccccc tttggtgtgg
     6901 gcggcgcact ggctcagcca tcttgccttc agttgggaaa gcccgatctg gcggcactgg
     6961 accgaagagt tcgcaaagga ccattccttc gtccactacg acgagcgcgg caacggcctt
     7021 tcggattggg acaaccccac gttctctgtt gatgcgtttg ttcgcgacct cgaagcggtg
     7081 gtcgatgcgc tcggcttcga tcgttttgcg ctcatcggta gctcgaaagg cggcgcgacc
     7141 gccatggcct atgcggcacg ccacccggag cgggtgtccc acctcatcct ctgcggtgcc
     7201 tacgcccagg gttggcgggt gtggggcaat gccgccgaaa tcgaacggcg cgaggcgatc
     7261 atcaccctga cgcgacaggg ctgggcgcag gacaaccccg cctttcggca gatcctgacc
     7321 tcgctgttgc tgcctgatgc cacggtcgaa gagatgggct cgttcaatga cctgcagcgc
     7381 atctcggcct cggccgagaa tgccgcccgg ctgctgcagt cgagtggaga cgtcaatgtc
     7441 ctcgaccttc tgccgggcat cgtcgcgccg acactc
//
LOCUS       DATNOI010000498        28055 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1137955,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000498 DATNOI010000000
VERSION     DATNOI010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 28055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28055
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137955"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VN968_20020"
     CDS             <1..838
                     /locus_tag="VN968_20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HXL31557.1"
                     /translation="FVAGHYCSSSSIPASEAYADGNVLQITPASTNPLYTERKLWNVA
                     RVCGRDDQQGLVAADYIAKVYKGKNVAILNDKTTYGKGLADETKKALNKAGFTEKMFE
                     SYNKGDKDFNAIVSRLKRDNIDLVYVGGYHQESGLLVRQMRDQGLKTVLMAGDALADK
                     EFASITGPAGEGTLFTFGPDPRNKPTAKAIVERFKAKNIDPEGYTLYTYAAMQVWSQA
                     VAKAKTTDPKKVMDTIKAGKWDTVIGKMEFDAKGDIKQIDYVVYKWDAKGNYTEINPK
                     GS"
     gene            1481..2476
                     /locus_tag="VN968_20025"
     CDS             1481..2476
                     /locus_tag="VN968_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HXL31558.1"
                     /translation="MKNLLTDIPGVRVGHADDAARASGVTAVMFDKPPVAAIDVRGGG
                     PGTREGALLELSSTVQRIDAIALAGGSAFGLEAGGGVQAWLAEQGRGFVVRGAIIPIV
                     PGAIMFDLLNGGNKAWGRFAPYRDLGYTAAAAASTSFALGSVGAGLGATTANFKGGIG
                     SASAVTQSGVRVAALAVVNAMGTVTVGDGPWFWAAPFEIDGEYGGRRLPPSFTPDMRR
                     MRIKGGAAATSVENTTLAVVVTDADLTNPQAKRLAMIAQTGFARAIYPVHAPLDGDVV
                     FAAATGEKPIDPLVGLTELGMVAANVVARAIARGVYTATALPFLGALPAWKDRFG"
     gene            complement(2510..3331)
                     /locus_tag="VN968_20030"
     CDS             complement(2510..3331)
                     /locus_tag="VN968_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HXL31559.1"
                     /translation="MLLALGAASSVIDAFKSLTQTKAKSSSPQATGFTQSSTNPFDTS
                     SSTSTAATPAPASGSSGFSQISPSTMSALLAAQSQSSTATTTSTSSTSTSPSGALQDL
                     FSQIDGNGDGQITKSEFESALGAGGTNTAQADDVFSKMDTNGDGKVSLEEMSSALKGS
                     KGGHHHHHVASSDGSSGATGDSSSTSGSSSDPLLQALDGATSTSTTNSDGSTTTSMTY
                     ADGSKVTMTTPASTSSASATSSGSATSSYNIIEQMIQRQAQAISSGATSSLSMSV"
     gene            4137..4838
                     /gene="rpe"
                     /locus_tag="VN968_20035"
     CDS             4137..4838
                     /gene="rpe"
                     /locus_tag="VN968_20035"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXL31560.1"
                     /translation="MSQQFASRPLVIAPSILASDFAKLGEEVRAVDQAGADWIHLDVM
                     DGHFVPNISYGPDVIKAMRPHTKKIFDAHLMISPCDSYLEAFAKAGCDHITVHAEAGP
                     HLHRSLQAIRALGKKAGVSLNPGTPISAIEYVIDMIDLVLVMSVNPGFGGQAFIHSAL
                     GKVGDVRAMTAGRPIDIEVDGGVGPDTSGQLAAAGANAFVAGSAVFKGGTMESYKANI
                     SAIRNAASLARGEAV"
     gene            complement(5938..7356)
                     /locus_tag="VN968_20040"
     CDS             complement(5938..7356)
                     /locus_tag="VN968_20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/universal stress protein"
                     /protein_id="HXL31561.1"
                     /translation="MAKPSVKTSVKPSVASGAVVDGFTVGECVHHGGMATLWSVTHPD
                     IAVPLLMKIPRVSEGEDPAAIVSFEMEQMILPRLSGPHVPACFGTGDFAAQPYVVIER
                     IPGKTLYSRLGDLPQSFEEASGIVGKIAVALADLHRQNVIHHDIKPSSIMFRPSGEVV
                     LIDFGLSHHNQLPDLLQEEFRLPYGTAPYMAPERLLGVRDDPRSDLFSLGVLLYFFTT
                     GVRPFGESETRRGMRRRLWRDPHPPRQLRADYPPWLQEVVLRCLEIEPVWRYPTASQL
                     AFDLGNPDQIKLTARSERVKRDPISTVWRRRFNRGLTQPKPKSDLAAQLAACPILAVA
                     LDTAEGSEPLNEALRVTAQRILATLPSARLACLNVLKLGRVTIDRTLDEQGNNKHIDR
                     LVMLKHWASPLKLDENRLTVHVLEAIDPAAAILEFAEVNHVDHIVIGARQNSLVRALL
                     GSVSAKVAAEAPCTVTVVRPPR"
     gene            complement(7360..8100)
                     /locus_tag="VN968_20045"
     CDS             complement(7360..8100)
                     /locus_tag="VN968_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXL31562.1"
                     /translation="MRLAIFADIHANRQAFSACLDFARARGAERMICLGDYVGYGADP
                     EWTVETVMGLVNDGAIAVRGNHDNAIGATAESMNAEAQAAIEWTRGRLSAPQRRFLAE
                     LPFELQEEDRLYVHSEASNPSKWHYVRSASDAARSIVATSAHITFCGHVHQPALYSMS
                     SAAKMTSFVPISGVPVQLLGGRRWLAVLGSVGQPRDRDPAASFAMFDTHSREISYCRV
                     PYDIEAAASRIRANGLPLWLADRLLVGR"
     gene            complement(8292..8882)
                     /locus_tag="VN968_20050"
     CDS             complement(8292..8882)
                     /locus_tag="VN968_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HXL31563.1"
                     /translation="MNENVVLTPERILEVTEDVLRRFGLAKATVVDVARALDVSHGSV
                     YRHFPSKASLREAVAKRWLDRVSAPLAKIAEASGPAPARLERWLRALFAAKHKKVSDD
                     PEMFATYLTLAREACKVVKAHKDILVDQVAHILSDGVKQGAFQVADVKASARAIFDAT
                     IRFHHPAHAEEWSDPQFAARIDAVLALLLRGLEAPR"
     gene            complement(8879..9202)
                     /locus_tag="VN968_20055"
     CDS             complement(8879..9202)
                     /locus_tag="VN968_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31564.1"
                     /translation="MTIEIFYLTEQLNRWLNLQVAHHLEAQATRLKAAKGPVLITSPK
                     AHGKLGADGPGGEVGLDCRGMPDCGESVEEAEGMDAIPDAAETTRFPAAGMAISDGEK
                     PTVSA"
     gene            9437..10744
                     /gene="purB"
                     /locus_tag="VN968_20060"
     CDS             9437..10744
                     /gene="purB"
                     /locus_tag="VN968_20060"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXL31565.1"
                     /translation="MISRYTRPEMAAIWEAQTRFKIWFEIEAHAADALAELGVIPKEA
                     ARAIWAKARGAIFDVARIDEIERETKHDVIAFLTHLAEIVGPEARFVHQGMTSSDVLD
                     TCLNVQLTRAADLLIADVDKVLAALKKRAFEHKMTPTIGRSHGIHAEPVTFGLKLAYA
                     YAEFTRARERLVTARNEVATCAISGAVGTFAQIDPRVEEHVAKAMGLQPEPISTQVIP
                     RDRHAMYFATLGVIASSIERLATEIRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVL
                     SENLTGLSRMVRAYVTPAMENVVLWHERDISHSSAERMIAPDATVTLDFALNRLAGLV
                     EKLLIYPENMQKNLDRLGGLVHSQRLLIALTQKGCSREDAYKLVQRNAMPVWRGEGDF
                     LTLLKEDAEVKKYLSNAEIEEQFDLGYHFKHVDTIFKRVFGEA"
     gene            complement(10935..11774)
                     /locus_tag="VN968_20065"
     CDS             complement(10935..11774)
                     /locus_tag="VN968_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HXL31566.1"
                     /translation="MLAALIIVFREVFEAGLIIGIVLAVTRSVPHRNQWVGGGVLAGV
                     LAACTVAAFAGALSNLFAGMGQELFNAAILCVAVAMLTWHNVWMARHGSELAGELRSA
                     GLAVVEGSKSLLALAIVVGVAVLREGSEVVLFLYGVVAAGDDSTWSVSLGGFAGLALG
                     AMICLLTYFGLLRIPVRALFTTTTVLIALLAAGMAAQATHFLEQANWLTAMDTIVWDS
                     GWILPDTSFVGRALHTLIGYTDQPTAMQLTVYLAILLVTFVLMRLYGSPAKATPAPAP
                     AQS"
     gene            12050..12847
                     /gene="murI"
                     /locus_tag="VN968_20070"
     CDS             12050..12847
                     /gene="murI"
                     /locus_tag="VN968_20070"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HXL31567.1"
                     /translation="MASSPTILVFDSGLGGLTVLREVVKVRPDAHYIYVADDAFFPYG
                     HHGEDQIIARVVPMIGELIATHAPDLIVIACNTISTLVMSHLRDAYKVPFVGTVPAIK
                     PACASSITKRVSVLGTKGTVQREYTRALIRDFAQGCDVTLVGSPDLASLAESALSGGE
                     ISDSEIFAEIAPCFVGDSASDPTRTDTVVLACTHYPLLMDRLVKLAPWPVDWVDPAPA
                     IARRVTDLLKVPGGEADHTGAQVIFTSRRPHTLIPALMPFFGGRVPA"
     gene            12937..13713
                     /locus_tag="VN968_20075"
     CDS             12937..13713
                     /locus_tag="VN968_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXL31568.1"
                     /translation="MSAIPEPLNRLRRQWREGRPTFGAIATIPSIQTVQIMARSGFDW
                     IIVDLEHGPIDLGSAHGMIVATSGTPCVPLARIAANEPWLAKAPMDIGALGINFPMIC
                     SREDAEKAVRSVRYPPRGDRLWGPFHAPFRWGVSMPDYMATADDDMICMITIEHVEAV
                     NRIDEIMAVPGIDVAVIGPGDLATSIDKRGHVDDPEVQALMARAEAGILKSGVPIGGV
                     ARTPQQANQMIERGYLALALGFDWSLFARGIAASFEGIIR"
     gene            13934..14089
                     /locus_tag="VN968_20080"
     CDS             13934..14089
                     /locus_tag="VN968_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31569.1"
                     /translation="MNAISTFLIHAIPETNTEIHPLRTIVLFCCFGLVASLCLAISGY
                     DLGAGLI"
     gene            14495..15274
                     /locus_tag="VN968_20085"
     CDS             14495..15274
                     /locus_tag="VN968_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXL31570.1"
                     /translation="MRGTVRKISMPRRLVADLMHASIKIPFVSLRRPLNVRPLLEVRA
                     LAAQPPGWAAIFVKAFSLVAKDEPVLRTLYAKWPWPSFYELPRSIAMVAIARVEDGQD
                     CVLPQKVPAADTVSLIDVDAQIRHAKDAPISEVPTFRKMLRVTRLPLPLRRFMWLVGL
                     NFGRQRANWCGSFGVTSVAAYGAGELHALSPGPFILSYGVVEPNQTIDVVIRWDHRIT
                     DAALIAKALTRLEQVLNTEIAAELRGARQQPEPKPVRAVAT"
     gene            15661..16278
                     /gene="rpsD"
                     /locus_tag="VN968_20090"
     CDS             15661..16278
                     /gene="rpsD"
                     /locus_tag="VN968_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXL31571.1"
                     /translation="MTKRSEAKYKIDRRMGQNIWGRPKSPVNRREYGPGQHGQRRKGK
                     LSDFGVQLRAKQKLKGYYANISERQFHGIYVEAGRMKGDTGENLIGLLERRLDTVVYR
                     AKFVATMFAARQFINHGHVKVNGRRVNISSFKLKVGDVIEVKESSKQLTVVLEASQLS
                     ERDVPDFLEVDHGKMTAKFARIPALSDVPFAVQMEPHLIVEFYSR"
     gene            16453..17541
                     /locus_tag="VN968_20095"
     CDS             16453..17541
                     /locus_tag="VN968_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HXL31572.1"
                     /translation="MTYTPPPRDPNAPPIRINLLSDTQTRPTPAMREAIARAEVGDEQ
                     IGDDPTVNLLCERVADLLGKQAAVFLPSGTMCNVAATLVHCRPGDEILAHETAHIIAR
                     EGGAHAALGGFQITPLKGADGKFSPDTFRAALHPRSRYQPPQTVVSVEQTANIGGGTI
                     WNKADLDDVVKIARSGGLATHMDGARLMNACVASGIAARDMTAGWDSAWIDFSKGLGA
                     PVGAVIAGSRDFIDEVWRWKQRLGGSMRQAGICAAACVFALDHHVDRLADDHANARAL
                     ARGLSQIAGIEVQQPETNLVFFNPDGAGVTGDEMVEALRKRGVRLAMMDGRIRACTHL
                     DVSAAMIEETIGLVRETVRGREPHLPVA"
     gene            complement(17602..19227)
                     /locus_tag="VN968_20100"
                     /pseudo
     CDS             complement(17602..19227)
                     /locus_tag="VN968_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-(1-6) glucans synthase"
     gene            19376..20869
                     /locus_tag="VN968_20105"
     CDS             19376..20869
                     /locus_tag="VN968_20105"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HXL31573.1"
                     /translation="MPFKRSSRRFSRRQFARIAGWSALGMSMLSAGSSLAELDQENRT
                     QNRTAASGFPNGFLWGTATSAYQIEGAVNEDGRGPSIWDRFAHTRGTISDHCNADTAT
                     DHYHLYKEDVQLMKALGAKAYRFSIAWPRVFPEGAGSPNPKGLDFYNRLLDELSANGM
                     EPFATLYHWDLPQTLQDRFGGWTSRDTSKAFADYAGHVAGRLSDRVKHFFTINECSRL
                     VHLGHGLGADAPGLKLSQSGLNQVRHNVALGHGLAVQAIRAHGSTGTRVGPAENAVSC
                     IPAIETPANIRATEMATRELNAGYLTLMLEGKYTDAYLAQAGKDAPKYTAEDLRVISS
                     PIDFVGLNVYTPDHYVIAADNERGFTLAPFPASFPHMKAAWLRPGPEAMYWVPRHVAK
                     LWNVKSIYITENGTSGSDQPAADGTIYDVDRIMYLRNHLTQLQRATSEGVPVLGYFLW
                     SLMDNFEWSDGFEQRFGLYHVDFATKRRTPKLSASFYRETIARNAVV"
     gene            complement(20881..22176)
                     /locus_tag="VN968_20110"
     CDS             complement(20881..22176)
                     /locus_tag="VN968_20110"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXL31574.1"
                     /translation="MKRWRTNGAAARCLVVAALAILLAAGWLHEVRAEPNAPTFSRTA
                     LEQIGNYIRNEVTTSKIPGAVLLIQQHGKPVYLQSFGVRDPATGQPMTSDTIFQIYSM
                     SKAVTSVAAMMLVDDGKLALNDPVSKYIRAFADAKVGVDLSDEAGQYPLKLEPLKRPI
                     TIRDLLRHTSGITYGFFGETAVQKLYANPQLYAGDFDNAEFADRIATLPLADQPATRW
                     NYSHSTDVLGRVVEVASGQTLFQFEKQRLFDPLGMSETAYYVADEAKWPRIAQPFPID
                     RLRVAGIKDPILPRRWESGGAGLVSTIGDYARFLQMLLNGGKLDGKRYLKPETVALMT
                     SDQIGPETKIIHDPFYFPGPTSGFGLGFAVRTSPPPNTTWPLGEYRWDGAGGSFYFVD
                     PQDDMLVVCMVQAPTQGGRIQLALKTMMFEALGKGLRKD"
     gene            complement(22157..23443)
                     /locus_tag="VN968_20115"
     CDS             complement(22157..23443)
                     /locus_tag="VN968_20115"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXL31575.1"
                     /translation="MNSGVVLRALICGALLTLAAAPSAMAEGTFDIPAGAHFNPQKLD
                     TIGEFFRNEVTTGKISGAILLIQQHGKPVYHQSFGVQDVVTQAPITDKTIFRLSSLTK
                     AITSVVAMQLIQDGKIKLDDPVSKYIPSFANMKVGEEKKAEDGTKTLELVTLTRPITI
                     LDLMRHTSGITYGFYGDSLVRKAYAAANLYAGEFDLAEFAERIAKLPLHNQPGALWQY
                     GHSTDILARIMEIVSGKSLLEIERGKLLDPLGMTDTGFFVTEPERLQRLAQPLPNDSN
                     FRVGREYRTEVRQKWESASGGMVSTMSDFARFAQMLTNGGTFEGRTYLSPKTFELMTS
                     DHAGKGSGVARDHYYFPGDGFGMGLGFAVRTDPGNAKPPPPGSLGELKWDGAAGCYLV
                     IDRKQDMFFVVLEQTPTERQRVQRTLKQLVYEALEN"
     gene            complement(23488..24042)
                     /locus_tag="VN968_20120"
     CDS             complement(23488..24042)
                     /locus_tag="VN968_20120"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HXL31576.1"
                     /translation="MSQGARLIGTARLEVIVGDITTLGVDAIVNAANTSLLGGGGVDG
                     AIHRAAGPGLLAECRALGGCETGDAKITSGYRLPARHVIHAVGPVWHGGSQSEDEALA
                     SCYRRAIELGQTHGLASMAFPAISTGVYRFPAGRAAGIAVSATAEALAAAPVITRIIF
                     CCFSDDSARLHAQAMAQLGSPCAD"
     gene            complement(24039..24377)
                     /gene="grxD"
                     /locus_tag="VN968_20125"
     CDS             complement(24039..24377)
                     /gene="grxD"
                     /locus_tag="VN968_20125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HXL31577.1"
                     /translation="MSIEQFIDNEVKSNDVVLFMKGTPQFPQCGFSGQVVQILDHVGV
                     GYKGLNVLESADLRNGIKTYSNWPTIPQLYVKGEFVGGCDIIREMFQAGELQQLLADK
                     GVTVSAAASA"
     gene            24658..25956
                     /gene="egtB"
                     /locus_tag="VN968_20130"
     CDS             24658..25956
                     /gene="egtB"
                     /locus_tag="VN968_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HXL31578.1"
                     /translation="MTKPASAAATLAAPCPSLFSDSGSLAEDLVEAYLTVRNETECRA
                     DPLTPEDQLIQSMPDASPAKWHRAHTTWFFEQFLLGEHCRGYKPFHPDYAYLFNSYYV
                     SAGPRHARHQRGHLTRPGADEITAYRRHVDAAVVKFFQTAGEDALTAIAPLVGVGLNH
                     EQQHQELMLTDILHAFAQNPIPPAYDPAWSFPVSSRAGDEKPRAGDEKPRAGDEWVTL
                     SEGIHTVGHTDDTFHFDNEKPAHRALVGPVKLARNLVTNAEWLAFMRDGGYTTPTLWL
                     MDGFAVVGNEQWQAPGHWRQVDGEWRIMTLGGLQPVDPSAPVCHVSYYEADAFARWSG
                     RHLPTEMEWEVAARAGQLNDAFGIVWQWTRSAYAPYPGYRAIEGALGEYNGKFMVNQL
                     VLRGSSLATPDGHSRITYRNFFYPHHRWQFTGLRLADYAI"
     gene            26004..26975
                     /gene="egtD"
                     /locus_tag="VN968_20135"
     CDS             26004..26975
                     /gene="egtD"
                     /locus_tag="VN968_20135"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HXL31579.1"
                     /translation="MNVHAAALAKTHRFDEQTSAFASDVIDGLSQHPKRLSPKYFYDA
                     TGSELFEQITLLPEYYPTRTELTILRDRASEISAVVPKGAALVEFGAGATTKVRLLLN
                     QCAFGAYVPVDISGDFLKAQADALRKDFPALDVYPVAADFTAPFALPDAVQAMPRLGF
                     FPGSTLGNFEPHEACAFMRSARDILGEAAQMIIGVDLEKNERVLYNAYNDAAGVTARF
                     NLNVLVRINRELGGNFDTAGFTHRAIYNRDRHRIEMHLISRKTQTVRALGRSFSFRAG
                     ESIHTESSYKYSLERFTALARGSGWSSRHSWTDKAGMFSVHALVASD"
     gene            complement(26951..>28055)
                     /locus_tag="VN968_20140"
     CDS             complement(26951..>28055)
                     /locus_tag="VN968_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HXL31580.1"
                     /translation="IGRFTPLGALQTSLLFLAVWWVWVYTSWITNWLNPEVTPVRILL
                     FLLMLGGLVLSTSIPKAFESRGLWFATAYAAMQVGKTVFLWVSTPPSRNLARLNAVRI
                     TAWLAMSAIFWIAGGFAEGNWRLALWAIALGIEYLSPAVRFWIPKYGASSIEDWMVEG
                     GHMAERCAGFIIIALGESIVVTGATFADLAWTTETVVAFVSAFIGSLAMWWIYFHKGV
                     EAGSELISKSSEPGRLARLAYTYLHMPIVAGIILSAVADELVLTHPADHSDPRTVFTA
                     IGGPLLFLLGTILFKHTIRGFLQLSHGVGIVALGGLAWFAGELSPLLLSIATTAIMII
                     VAVWESISLKSGPAESRTVGSPTAESQSEATSA"
BASE COUNT         5514 a         8865 c         8518 g         5158 t
ORIGIN      
        1 gttcgtggcg ggccattatt gttcgtcctc gtcgatcccg gcttcggaag cctatgcgga
       61 cggcaacgtg ctgcagatta cgccggcctc gaccaacccg ctgtacaccg agcgcaagct
      121 ctggaacgtg gcgcgggtct gtggccgcga cgatcagcag ggcctggtcg ccgccgatta
      181 catcgcgaag gtctacaaag gcaagaacgt cgccatcctc aacgacaaga cgacctacgg
      241 caaaggcctc gccgacgaaa ccaagaaggc gctcaacaag gccggcttca ccgaaaagat
      301 gttcgagtcc tacaacaagg gcgacaagga tttcaacgcc atcgtgtcgc gcctgaagcg
      361 cgacaatatc gacctcgtct atgtcggcgg ctaccatcag gaatccggat tgctggtccg
      421 tcagatgcgc gatcagggcc tcaagaccgt tttgatggcc ggcgacgcgc tggccgacaa
      481 ggagttcgcg tcgatcaccg ggcccgccgg cgaaggcacc ctgttcacct tcggtcccga
      541 cccgcgcaac aagccgaccg caaaggccat cgtcgagcgg ttcaaggcca agaacatcga
      601 tccggaaggc tacacgctct acacctacgc cgcgatgcag gtctggtcac aggcggtggc
      661 gaaagccaag acaaccgatc cgaagaaagt catggatacg atcaaggccg gcaagtggga
      721 tacggtgatc ggcaagatgg agttcgacgc caagggcgac atcaagcaga tcgactatgt
      781 cgtctacaaa tgggacgcca agggcaacta caccgagatc aatccgaagg ggtcgtaagg
      841 ccctatagat tctgtcgtga catcaacgcc ccggttcgcc ggggcgtttt tgttgaaggg
      901 tttgccgatg agaatgcatc gtcacgggta gccgcagcac cgaggaacca acgcatgctt
      961 gcgagccggc cgagttcgaa gaccatccac ttgaatgccg cgaccgcgct tacgcgcgat
     1021 catcacggcg atctgtttct gggcggcatc atcgagggac catgaaagtg ggcgatccgg
     1081 cccgttgcag agtttttcta cgaaaacgag tttggcaaat ctgaaacgat atcgggcttg
     1141 atcagcctca tctggcgggt gcgcgacaat ctcagtttcg acgtcgggct gcggcatgct
     1201 ctcacgaatg gccatcaggt aaatgaagtg cgtgcagggc tgacgttcgg ctttcccctg
     1261 gggcaattcg gagggcgctt ggcgcaatcc agataatctt aacgttcgaa atcaaagcgc
     1321 ctgcatgtaa atgcgtctcg atgggatcaa actgaccgtc cctccctgtt gacgcacgcg
     1381 atatcaacgc ggtatccccg cttcagagaa caacttccat cacccacaac cccctcctca
     1441 agaacgcaag gagtgaggga aagggagccg gtcctctcct ttgaaaaacc tcctcaccga
     1501 cattccaggc gtccgcgtcg gccacgccga tgatgcagcg cgggcgtccg gcgtcacggc
     1561 ggtcatgttc gacaagccgc cggtggccgc gatcgacgtg cgcggcggcg gtcccggcac
     1621 ccgcgaaggc gcgctgcttg agctatccag caccgtgcag cggatcgacg ccatcgcgct
     1681 cgccggcggc tcggcgttcg ggctcgaggc cggcggcggg gtacaggcgt ggctcgccga
     1741 acagggccgc ggctttgtgg tgcgcggcgc gatcattccg atcgtgccgg gcgcgatcat
     1801 gttcgatctg ctcaacggcg gcaacaaggc ctggggccgc ttcgcgccct accgcgatct
     1861 cggctacacc gcggccgccg cggccagcac cagtttcgca ctcgggagcg tcggcgccgg
     1921 gctcggcgcg accaccgcga atttcaaggg cgggatcggc tcagcatcgg cggtaacgca
     1981 aagcggcgtc cgggtcgcag cgcttgcggt cgtcaatgcg atgggcaccg tcacggtcgg
     2041 cgatggacct tggttctggg cggcgccgtt tgagatcgac ggtgaatatg gcggacgcag
     2101 attgccgccg tcattcacgc cggacatgcg caggatgcgc atcaaaggcg gcgcggccgc
     2161 gacatcggtg gaaaacacca cgctggcggt ggtcgtcacc gacgccgacc tgaccaaccc
     2221 ccaggccaag cggctggcga tgatcgcgca gaccggattt gcccgcgcga tctatccggt
     2281 gcatgcgccg ctcgatggag atgtggtgtt cgccgccgcg accggcgaaa agccgatcga
     2341 tccgctggtc ggcctcaccg agctcggcat ggtcgcggcc aatgtcgtgg cccgcgccat
     2401 cgcgcgcggg gtttacacgg cgaccgcgct gcccttcctg ggcgcgctgc cggcgtggaa
     2461 ggaccggttc ggctaggtca cggtgttctc aacggttcgc caagctgtct cagacgctca
     2521 tcgagagcga tgaggtcgcc ccggatgaaa tcgcctgtgc ctgacgctga atcatctgct
     2581 cgatgatatt gtaggatgaa gtcgcgctgc cggatgaggt ggcggaggcg gatgacgtcg
     2641 acgcgggcgt ggtcatcgtc accttggacc cgtcggcata cgtcattgat gtggtggtcg
     2701 agccgtcgct gttggtggtc gacgtgctgg ttgcgccgtc caatgcctgc agcaggggat
     2761 cggagctcga tccgctggtg ctgctggaat caccggtagc gccgctcgat ccatccgaac
     2821 tggcgacatg atggtgatgg tggccgccct tgctgccctt cagcgccgac gacatttctt
     2881 cgagactgac tttgccgtcg ccgttcgtgt ccattttgct gaacacgtca tctgcctgcg
     2941 cggtgttggt gccgccggcg ccgagggcgc tctcaaattc ggacttggtg atctggccgt
     3001 caccgttgcc gtcgatctgc gagaacaggt cttgcagggc gccggacggg ctggtcgatg
     3061 tcgacgatgt cgatgtcgtt gttgcggtcg atgactggct ttgcgccgcc agcagcgcgc
     3121 tcatcgtcga cggcgatatc tgcgaaaacc cgctggaccc ggacgccggg gccggagtgg
     3181 ccgcagtcga ggtactactg gaagtgtcga acgggttcgt cgagctctgg gtaaaaccgg
     3241 tggcttgcgg agacgacgat ttcgctttcg tttgggtcag cgacttgaag gcgtcgatta
     3301 cggacgacgc ggcgccaagg gcgagcagca tagcggaact ccgaaaaatg ccgcatcgcg
     3361 cggctgttgc ttcgacaccg attgatcagc aagcgccgtg ccaccgcgaa aaacccaata
     3421 aaataagggt ttttcgcatc ccaagcggtc ccccctcccc ggcaatagat gccgctgcgg
     3481 cagaattttc cgcccatcga cgtgccccgg gcgctgctta atgtcctcgg gtccgcgctc
     3541 ggcgcggccg tgctcgcgtt ccccgcggca tacagggaac aaatagcgcc gccaaaacga
     3601 ggcggtccac ccaatcaaga ttgaaggaac agtttccgag ggaaaaaatc aggacgaagt
     3661 atcgtcatct tcgattggcg cgatactggc gatctccgtc gggaatatgc tctcgtatat
     3721 cgcgcgcgcc tcttgaatga gacgggccgc ccggtcgggg gcaggcttcg ggatgaattg
     3781 tacgacttcg cccatttgct caccgaggca ggaagctcgg aggaaatatt ccgccaatga
     3841 ccaccagcgc cagtcacaat gcgaatatcg tgccaaggac ctcccataac gaacgcaagc
     3901 cagaaaaact gggatcggaa ccggggcgag cgatcgcggg cggggaactg cgggggtatg
     3961 ctgaaacaac gttacctgga cccaagcccg agcgcgcgcg ccggacggga ttcgcaccgc
     4021 cgagggcgcg cgtcgcgccg cggacgcggg tggagtcttc cccgcattgc ccccaagctt
     4081 tgcgcgtgtt acccgaaaac tgctccacgc ttcgaaaatg gttcgggaaa gcgcacatgt
     4141 ctcaacaatt tgcgtcacgc cccctcgtca tcgcgccgtc gatcctggcg tccgatttcg
     4201 ccaaactggg cgaagaggtt cgcgccgtgg accaggccgg cgccgactgg atccacctcg
     4261 acgtgatgga cggccatttc gtccccaaca tttcctacgg ccctgatgtc atcaaggcga
     4321 tgcgcccgca caccaaaaaa atcttcgacg cgcatctgat gatctcgccg tgcgattcgt
     4381 atctggaggc cttcgccaag gccggctgcg atcacatcac ggtgcatgcc gaggccggcc
     4441 cgcatctgca tcgctcgctg caggcgatcc gggcgcttgg caagaaagcc ggcgtctcgc
     4501 tcaatccggg cacgccgatc tcagcgatcg aatatgtgat cgacatgatc gatctggtgc
     4561 tggtgatgtc ggtcaatccc ggcttcggcg gccaggcttt tattcattcg gcactcggca
     4621 aggtcggtga cgtcagggcg atgactgccg ggcgcccgat cgacatcgag gtcgacggcg
     4681 gcgtcggccc ggacacttcc ggccagttgg ccgccgccgg cgccaatgcc tttgtcgccg
     4741 gctccgccgt gttcaagggc ggcaccatgg aatcttataa ggccaatatt tccgcaatcc
     4801 gcaacgcggc gtcgctggcg cgtggcgaag cggtctagac tgtcccgtga attgctcgcg
     4861 gttcctgctt ctgcgcggga atccaatctt tgcccgattg actcgagcca atttttgcgc
     4921 atgagccgcc agcacgcctc catggtaccc gagaagatgc catggcccgg cagactgtat
     4981 cggaaacctg gtgcggctga atccggagaa gagaacatat ccgcaagatc aagcgagttc
     5041 ccggtcacct caccagaggc cgccgacgca gtcgtcgacg ttctgagcga tgtcagcaac
     5101 ttgagcaaat cgacttgcga tgatgcggca cccaaagcaa acaacatggc atcactcgcg
     5161 acgacggaag cacggctcat agctacccgg tgaggcggag caaacaccgt gccagcgagg
     5221 gaacttgata aaaaagtggt tctgctcgat tggcgtgccg gaactctggg caaatcgtgc
     5281 cgcggcggaa gaacttgcgc ctcacatgcg cgatgccgcc ttaatagaca attcgattcg
     5341 ccgggttcct gcgtgactaa tctttgcgct acaacccgta gtcgtccggt gtttcaaaat
     5401 ctcgactctc tgttacacaa tttttgcgag cattttgacg gagcaatcac cacgtggact
     5461 ggcattgcgg tgtgatttgg attgaacatc gcattcggag cccgatcgaa cagcccgtcg
     5521 cttgcagtga acctcgtttg caccacggtt gatgacatgc aaggcatcct tccaaaccgg
     5581 catttgaagc gtcgccgctt attgccgaga cggccacatc ccgacattga atttctgttc
     5641 cgtcggcacg acaggatgag ccatagcatg gctcgacatg acgacagcag cgaaagcggc
     5701 cgggaatccg gacatcagga gacgcccagc cgcaatcggc tgcggccatg gtccattttc
     5761 tcgtaaaccg agcgtcggtc gccgcttttg cgggtgatct atgaattcgg tttgcccaag
     5821 ctgcctgacc gagggtagag cgggatgacg attcgaagaa aagtcatccc gctttagtta
     5881 ggctttcacc tgatccggat ttttgctgct cgcattctcc tgctcatgca gcgccgccta
     5941 ccgcggcggc cgtacaaccg tcaccgtaca gggcgcctcc gccgccactt tggcggagac
     6001 gctgccgagc agcgctcgca caagcgaatt ctggcgcgcg ccgatgacga tatgatcgac
     6061 atgattgact tcggcgaatt ccaggatcgc cgccgcggga tcgatggcct cgaggacgtg
     6121 cacggtgagc ctgttttcgt cgagcttgag cggcgacgcc caatgcttga gcatgaccag
     6181 ccggtcgatg tgcttgttgt tgccctgctc gtcgagcgtc ctgtcgatcg tgacgcggcc
     6241 aagtttcaag acattcaggc acgctagtct tgcggatggc agggtcgcca ggattcgctg
     6301 ggcggtgacg cgcagggcct cgttcagtgg ctcagatccc tccgcggtat cgagcgcgac
     6361 tgccagaatc ggacatgccg ccagctgcgc cgccagatcg gatttcggct tcggctgcgt
     6421 caggccgcga ttgaaacggc ggcgccacac ggtgctgatc ggatcgcgct tcacccgctc
     6481 cgagcgcgcg gtcagcttga tctggtcggg gttaccgagg tcgaatgcca gttgcgatgc
     6541 cgtggggtag cgccacaccg gctcgatttc gaggcaccgc agcacgactt cctgcagcca
     6601 gggcggatag tcggctctca gttggcgcgg cggatgcgga tcgcgccaca gccgccgccg
     6661 catgccgcgc ctggtctcgc tctcgccgaa cggccgcacg ccggtggtga agaagtacaa
     6721 aagcacgccc aacgaaaaaa ggtcgctgcg cggatcgtcc cgcacgccaa gcaaacgctc
     6781 cggtgccata tacggcgccg tgccgtaggg cagccggaat tcttcctgca gcagatcggg
     6841 caactggtta tgatgcgaca agccaaaatc gatcaggacc acctcgcccg aggggcgaaa
     6901 catgatgctg ctcggcttga tgtcgtgatg gatgacattc tgtcgatgca ggtcggccag
     6961 cgccactgcg atttttccga caatgccgct cgcctcctca aagctctgcg gcagatcgcc
     7021 aagcctgctg taaagcgtct tgccgggaat gcgctcgatc acgacataag gctgggccgc
     7081 gaaatcgccg gtgccgaaac aggccggcac gtgaggcccc gacagccgcg gcaggatcat
     7141 ctgctccatc tcgaaactga cgatggcagc cgggtcctct ccttccgaaa ccctcgggat
     7201 tttcatcaac agcggcacag cgatgtcggg gtgggtcacg ctccacagcg tcgccatgcc
     7261 gccgtgatgg acgcattccc cgaccgtgaa accatcgacg acggcgcctg atgcgaccga
     7321 gggcttaacc gaagtcttaa ccgaaggctt ggccatcgct cacctcccga ccaagagccg
     7381 atcggccagc cagagcggca ggccgttggc gcgaatccgg ctcgccgctg cctcgatatc
     7441 gtagggcaca cggcaatagc tgatttcccg ggagtgggta tcgaacatcg caaacgaagc
     7501 tgccggatcg cgatcgcggg gttggcccac cgagccgaga acggccagcc agcgccgccc
     7561 cccgagcaat tgcacgggga cgcccgatat cgggacgaag ctcgtcatct tcgcggctga
     7621 agacatcgaa tagagcgccg gctggtgaac atgaccgcag aacgtaatgt gagctgacgt
     7681 cgccacgatg ctgcgcgcgg catccgatgc actgcggacg taatgccatt tggagggatt
     7741 gctggcttcg gaatgaacgt agaggcgatc ctcctcctgc agctcgaacg gcaattccgc
     7801 cagaaagcgc cgctgtggcg cgctcagccg gccgcgcgtc cactcgatcg cggcctgggc
     7861 ttcggcattc atcgattccg ccgtggcgcc gatggcgttg tcgtggttgc cgcgcacggc
     7921 gatggcgcca tcattgacga ggcccatcac cgtctcgacg gtccattccg gatcggcgcc
     7981 atagccgacg taatcgccga ggcaaatcat ccgctccgcg ccacgcgccc gcgcaaaatc
     8041 gaggcacgcg ctgaacgctt gccggttggc gtggatatcg gcaaaaatgg ccagccgcac
     8101 gctttcctcc accgccatcc tatcggccgg gcctgtaccg gatttgatgg atatcaagca
     8161 acaaagccgg ccggccgcaa tcgggccgac ggaggaattg cggtgatgca ggggacaaga
     8221 gcgcttctgc gatccgcggg ccgcgccggc gatggcgcac ggaagagatg atttggagag
     8281 atgatctagc gctagcgcgg tgcttccagg ccgcggagca acagcgccag cacggcgtcg
     8341 atccgcgctg cgaactgcgg gtcgctccat tcctcggcat gcgcgggatg atgaaaacgg
     8401 atggtggcgt cgaaaatcgc gcgcgccgag gctttgacgt cggcgacctg gaaggcgccc
     8461 tgcttgacgc cgtcggacaa aatatgcgcg acttgatcga cgagaatatc cttatgcgcc
     8521 ttgaccacct tgcacgcctc tcgcgccagc gtcagatagg tcgcgaacat ctcggggtca
     8581 tcgctgacct tcttgtgctt ggctgcaaac agcgcacgca gccagcgctc cagcctggcc
     8641 ggcgcgggcc ccgacgcttc cgcaatcttt gcaagcggcg cgctgacgcg gtcgagccag
     8701 cgcttggcca ccgcttcgcg cagcgaagcc ttgctcggaa aatgacggta aacgctgccg
     8761 tggctcacat cgagcgcacg ggcaacatcc accaccgtgg cctttgccag tccaaagcgg
     8821 cgcagcacgt cctcggtgac ttcgagaatt cgctctgggg tcaagaccac attttcgttc
     8881 atgcggacac cgtcggtttt tcaccatcgc tgatggccat ccctgcggca ggaaagcgcg
     8941 tcgtttcggc ggcgtccggg atcgcatcca taccctcggc ctcctcaacg gactcgccgc
     9001 aatcgggcat tcccctgcaa tcgagaccaa cttccccccc gggaccgtca gccccgagtt
     9061 ttccatgggc ttttgggctc gttatcagga ccggcccttt ggcggccttt aatctcgtgg
     9121 cctgtgcctc gagatgatgg gccacctgaa ggttgagcca gcgattaagt tgttcagtca
     9181 gataaaaaat ttcgatagtc atggggagtc ctctgcaagt cccggacacg cgggcgatca
     9241 gccaacgcgc aaaggtgaca ccaaacctgg cgccacctcc tgaatagcat atctgactga
     9301 cagattacaa tatctgttcg tgatcaggag ctcgggccgg ttccggttgc tggagcgtcc
     9361 gccggcggca gcggtttgtc tcaccggccc tgctctgcta aagcgcggct taatccccat
     9421 gagccacgag tcgtcgatga tttcccgcta cacccgcccg gaaatggccg ccatctggga
     9481 ggcgcagacg cgcttcaaga tctggttcga gatcgaggcg catgcggcgg atgcgctggc
     9541 ggaactcggc gtcataccca aggaggcggc aagagcgatc tgggccaagg cccggggcgc
     9601 catcttcgat gtcgcccgga tcgacgagat cgagcgcgag accaagcacg acgtgatcgc
     9661 cttcctcacc catctggcgg aaatcgtcgg ccccgaggcc cgtttcgtgc accagggcat
     9721 gacctcgtcc gacgtgctcg atacctgcct caatgtgcag ctgacccgcg ccgcggatct
     9781 gttgatcgcc gacgtcgaca aggttctggc ggcactcaag aagcgcgcct tcgagcacaa
     9841 gatgacgccg accatcggcc gctcccacgg catccatgcc gaaccggtga ccttcgggct
     9901 gaaactcgct tacgcctacg cggaattcac ccgggcgcgc gagcggctcg tcaccgcccg
     9961 caatgaagtc gcgacctgcg cgatctcggg cgcggtcggc acctttgccc agatcgaccc
    10021 acgggtggag gaacacgtcg ccaaagcgat ggggcttcag ccggagccga tctcgaccca
    10081 ggtgatcccg cgcgaccgcc acgcgatgta ttttgccacc ctcggcgtga tcgcgtcgtc
    10141 gatcgagcgc ctcgccaccg agatccggca cctgcagcgc accgaagtgc tggaagcgga
    10201 ggagtttttt tccgagggcc agaagggctc ctcggccatg ccgcacaagc gcaacccggt
    10261 gctgtcggag aatctcaccg gcctctcccg catggtgcgc gcctatgtga cgccggccat
    10321 ggagaacgtc gtgttgtggc atgagcgcga tatctcgcat tcgtcggccg agcggatgat
    10381 cgcgcccgac gccaccgtga cgctggattt tgcccttaac cgcctcgcgg gcctggtgga
    10441 gaagctgctg atctaccccg aaaacatgca gaagaacctg gatcgtttgg gcggtctcgt
    10501 gcattcgcag cggctcctga tcgcgctgac ccaaaaaggc tgtagccgcg aggacgccta
    10561 caaactcgtg cagcgcaacg cgatgccggt gtggcgcggc gaaggcgatt ttctgacgct
    10621 gctcaaagag gacgccgagg tgaagaagta tctcagcaat gctgagatcg aagaacaatt
    10681 cgacctcggc tatcacttca agcacgtcga taccatcttc aagcgcgtgt tcggcgaggc
    10741 gtgaacgccg agcatccggc aaatgggcag aggcagataa cgccgacgag caacaaaacc
    10801 catgctggct ggcgctaaat tgctggaaac gcaagttgca atcagagcgg gagtgtccgg
    10861 ttgctgcaaa gcagaaccgg aaaaaggcaa agccggccga agtccgcccg cgcgcagaac
    10921 cggaaatccg gcgctcagct ttgcgccggc gcgggcgccg gcgtcgcttt cgccggcgaa
    10981 ccatagagcc gcatcagcac gaaggtcacg agcaggatcg cgagatagac cgtgagttgc
    11041 atcgcggtgg gctggtcggt gtagccgatc agggtgtgca gggcgcggcc gacaaaactc
    11101 gtgtccggca ggatccagcc cgagtcccag acgatggtgt ccatcgcggt cagccagttc
    11161 gcctgttcga ggaagtgcgt cgcctgcgcc gccatgccgg cggccagcag cgcgatcaac
    11221 accgtcgtcg tggtaaacag cgcgcgcacg ggaatgcgca acaggccgaa ataggtcaaa
    11281 aggcaaatca tggcgccgag cgccaatccg gcgaagccgc cgagcgacac gctccaggtc
    11341 gaatcgtccc cggccgcaac gacgccgtac aggaacagca ccacttcgct gccctcgcgc
    11401 agtacggcga cgccgaccac gatcgcgagc gccaataatg acttcgaacc ctcgaccacc
    11461 gccaggcccg cggagcgcag ttcgccggcg agttcgctgc cgtgacgcgc catccacaca
    11521 ttgtgccagg tgagcatcgc gacggcgacg cagaggatcg ccgcattaaa cagctcctgc
    11581 cccattcccg cgaacagatt tgacaacgcg cctgcgaacg ccgcgaccgt gcaggcggcc
    11641 agcacgcccg cgagcacgcc gccgccgacc cattgattgc gatgcggaac ggagcgggtg
    11701 accgcgagca cgatgccgat gatcagaccg gcttcgaaaa cctcacggaa aacgatgatc
    11761 agcgcggcga gcacaacgcg tccccctgtt gcacgaccgt ctcgccgtgc gcggtgtcgg
    11821 ctttagcatg ggcttgacga acgtggtccc tcttcaaaaa aagccggcag gactacggct
    11881 gttagccgct ggccgctggc cgcgcaaaaa gagttgttct aatttggagc gattctaacg
    11941 aaacggcttc gctttgtcgt ttgtggccgg agcccggcca cccggcgcta gtgcaggtgt
    12001 gccgccaaaa acaactgaac ggtaaccccg gatcggctag gatcgcggcg tggcatcatc
    12061 tccgacgatt ctcgtgttcg attccggcct tggcggcctc acggtattgc gtgaggtggt
    12121 caaggtgcgg cctgatgcgc attacattta tgtcgcggac gacgcgttct ttccctacgg
    12181 ccatcatggc gaggaccaga tcatcgcgcg ggtggtgccg atgatcggcg aattgatcgc
    12241 cacgcacgcc cccgatctga tcgtgatcgc ctgcaacacg atctcgaccc tggtgatgtc
    12301 gcatcttcgc gatgcctaca aggtgccctt tgtcggaacg gtgccggcga tcaagccggc
    12361 ctgcgcgagc tccataacaa agcgcgtgtc ggtgctcggc accaagggca ccgtccagcg
    12421 cgaatacacc agggcgctga tccgcgattt cgcgcaaggc tgcgacgtga cgctggtggg
    12481 ctcgccggat ctggcctcgc tggccgaatc cgcgctgagc ggcggcgaaa taagcgactc
    12541 cgagatattt gccgaaatcg cgccgtgttt tgtcggggac agcgccagcg atccgacccg
    12601 caccgacacc gtcgtgctgg cctgcaccca ctacccgctg ctgatggacc ggctggtcaa
    12661 gctcgcgccg tggccggtgg actgggtcga tccagcgccc gcgatcgccc ggcgggtgac
    12721 ggatttgctg aaagtccccg gcggcgaagc cgaccacacc ggtgcacaag tgatcttcac
    12781 ctccaggcgg ccgcacacgc tgatcccggc gttgatgccg tttttcggcg gccgtgttcc
    12841 cgcctgaatc gatattgatc ttttcgacat cctgccgcgc tgttagtttt ttgggcgacg
    12901 cgcttccccc gccacccgga cggagtttcg gatttgattt ccgcgatacc tgaacccctc
    12961 aaccgtcttc gcaggcaatg gcgggagggg cgtcccacct tcggcgcgat tgcgaccatt
    13021 cccagcattc agaccgtcca gatcatggcc cgatcgggct tcgactggat catcgtcgac
    13081 ctcgagcacg gtccgatcga ccttggctcc gcgcacggca tgatcgtcgc cacctcaggc
    13141 acgccctgcg tgccgctggc gcggattgcc gccaacgagc cctggcttgc gaaagccccg
    13201 atggatatcg gcgcgctggg catcaatttt ccgatgatct gcagccgcga ggacgccgag
    13261 aaagccgtgc gcagcgtgcg ctatccgccg cgcggcgacc ggctctgggg accgttccat
    13321 gcgccgtttc gctggggcgt ctcgatgccc gactatatgg cgaccgccga cgatgacatg
    13381 atctgcatga tcaccatcga gcacgtcgag gcagtcaacc gcatcgacga gatcatggcc
    13441 gtgcccggca tcgacgtcgc ggtgatcggc cccggcgacc ttgcgaccag catcgacaaa
    13501 cgcggccacg tcgatgaccc cgaagtgcag gcgctgatgg cgcgcgccga ggccggcatc
    13561 ctcaagagcg gcgttccgat cggcggcgtg gcgcgcacgc cgcagcaggc caaccagatg
    13621 atcgaacggg gctatctggc gctggcgctt ggcttcgact ggtcgctgtt tgcccgcggc
    13681 atcgcggcga gcttcgaagg catcatacgc tgaagcgagc ggtacccgga gaggcaagcc
    13741 gggtgatgcc tttcctccca tcgccgtata atgacaccgg cagggagcac cacatcggca
    13801 attgtttcat ggccggctaa ccaaaaaaca attcgcgggt gccttcgaaa ccgtttttcg
    13861 ggaccgtgaa aacatttccg aaaccgacac ccaataccaa tgcgagcgcc aattttgaaa
    13921 tatggagctt gccatgaatg ccatttcgac ctttcttatc catgcgattc cggaaacgaa
    13981 tactgaaatc catccgctca ggacgatcgt tctgttttgc tgctttggcc tggtcgcttc
    14041 gctctgcctc gcaatttcag gctatgatct aggcgccggc ctgatctagg cggccttcga
    14101 tcgccctgtc agcgtcaccc tcgcgtgccg cacgatcgac ccggaccgat cgtcatcagg
    14161 gcttttttgg tccggtcgcg atcatttcaa gcccgatgct ggaaggcaaa gccgttctca
    14221 agatcgacgg caagcccgac ccacatcttt ggcctgcaac caaagcctcg cagatcccgg
    14281 ttccgcaccg gcaggccgtt gcgtgacgct tctgttcacg atgtcaacac cctgaaaatg
    14341 ctcatgattt gtgcatcggc caggacccgg ccgctcgcct cgcccgcgcc ataaaggcag
    14401 aaccatctgc agtcaggtag gttggttcgt gctagatcag agccggacgg ggtgatccga
    14461 ctcagccggt tgtagcagga acttgagtga aacgatgcgc ggaacggttc gaaaaatctc
    14521 aatgccgcgc cgtctcgtcg ccgacctcat gcatgcgtcg atcaaaatac cctttgtttc
    14581 gctgcggcgg cccctcaacg tccgtccact gctggaggtc cgggcgcttg ccgcgcagcc
    14641 gcctggctgg gcggcgattt tcgtcaaggc gttctcgctg gtagcaaaag acgaacccgt
    14701 cctgcgcacg ctttatgcca aatggccatg gcccagtttc tatgaactgc cgcgcagcat
    14761 tgcgatggtg gcgattgcgc gggtcgaaga cggacaggat tgcgttctgc cgcaaaaagt
    14821 ccctgcagcg gacacggtat cgctgatcga cgtcgacgcg cagatccgtc acgccaagga
    14881 cgccccgatc agcgaggtgc cgacttttcg caagatgctg cgggtaacaa ggctgccgct
    14941 gccgcttcgc cgtttcatgt ggctggttgg gctcaatttc ggacgccagc gcgccaactg
    15001 gtgcggaagt tttggagtga cttcggttgc cgcctatggc gccggcgagc tgcatgcgct
    15061 cagtccaggc ccgtttattc tcagttatgg ggtggtcgaa ccaaaccaga ccatcgatgt
    15121 cgtgatccgc tgggatcacc gcattaccga cgccgccctg atcgcaaagg ccctgacccg
    15181 gctggaacag gtgctgaaca ccgaaatcgc tgctgaattg cgcggggcca ggcagcagcc
    15241 ggagccaaag ccggtccggg cggttgcgac gtgagcggcg gccttcccaa ccgtcattcc
    15301 gggccgtcgc gctgggcgac gaacccggaa tctcgagatt cccgatgtgc aattgcacat
    15361 ctggggtctg gtccggagcc tgtcatcggg cggcgcaaag cgccgacccg ttgggaccat
    15421 cccggaatga cggttccccg gtccttcatt gacacaaccg ccgtttctcc ctagaacccg
    15481 cctgctcgcg ggccggtttt cggcccgcgt tgcgtttcgc gacccgtggc ctttcccttc
    15541 tgctttaagg gttgggcctg tcggctccgg gaaattcccg gcgcacagga gggcgcgttt
    15601 cctcaacttg aaacagaacg ccgtcgcatc cgcgatggct atgcttacca agaggacgcg
    15661 atgactaagc gcagtgaagc gaagtacaag atcgatcgcc ggatgggcca gaatatctgg
    15721 ggccggccga agagcccggt gaaccgccgg gaatatggcc ccggccagca cggccagcgc
    15781 cgcaaaggaa aactctccga cttcggcgtg cagttgcgcg ccaagcagaa gcttaaaggc
    15841 tactacgcca acatcagcga gcgccagttt cacggcattt atgtcgaagc cggccggatg
    15901 aagggcgaca ccggtgaaaa cctgatcggc ctgctcgagc gccgtctcga caccgtggtg
    15961 tatcgtgcca agttcgtggc cacgatgttt gccgcgcgcc agttcatcaa ccacggccac
    16021 gtcaaggtga atggccgccg tgtcaacatc tcgagcttca agctgaaggt cggcgacgtg
    16081 atcgaggtca aggaatcctc aaaacagctc accgtcgttc tggaagccag ccagctctcg
    16141 gagcgtgacg tgcccgactt cctcgaagtc gatcacggca agatgacggc gaagttcgcc
    16201 cgcatccccg ctttgtccga cgtgccgttc gcggtgcaga tggagccgca tctgatcgtc
    16261 gaattctatt cgcgttgatt tcttgaattg gcgttttcgt gagatcaagg ccccggtttt
    16321 gccggggcct tttgtttcgt catggccggg cttggtcctg ggcatccacg tctttatgat
    16381 cgaagacaag cagcaagaca tgggtggccg ggacgagccc ggccatgacg acgtcagtga
    16441 cgagacctga tcatgaccta tacccctcct ccgcgcgacc cgaatgcgcc gccgatccgg
    16501 atcaacctgt tgtcggatac ccagacccgc ccgacgccgg ccatgcgcga ggcgatcgcg
    16561 cgggccgaag tcggcgacga gcagatcggc gacgatccga ccgtgaacct gctgtgcgag
    16621 cgggtggccg atctgctcgg caagcaggcc gcggtgttcc tgccgtcggg caccatgtgc
    16681 aacgttgccg cgacgctggt gcattgccgg cccggcgacg aaattctcgc ccacgaaacc
    16741 gcccatatca tcgcccgtga aggcggcgcg cacgccgcac tcggcggatt ccagatcacg
    16801 ccgctaaagg gcgccgacgg aaaattttcg cccgatactt ttcgcgcagc gcttcacccg
    16861 cgctcgcgtt accagccgcc gcagaccgtc gtcagcgtcg aacagaccgc caatatcggc
    16921 ggcggcacga tctggaacaa agcggatctc gatgacgtcg tgaaaatcgc cagatccggc
    16981 ggcctcgcca cccacatgga tggcgcgcgg ctcatgaacg cctgcgtcgc ctcgggcatt
    17041 gccgcgcgcg acatgaccgc gggttgggat tccgcctgga tcgatttctc gaaagggctc
    17101 ggtgcgccgg tcggcgccgt gatcgccggc tcgcgcgatt tcatcgacga ggtctggcgc
    17161 tggaagcagc gccttggcgg ctcgatgcgc caggccggca tttgcgccgc cgcctgcgtt
    17221 ttcgcgctcg accaccacgt cgaccggctc gcggacgatc acgccaacgc ccgcgcgctg
    17281 gcgcggggtc tatcgcaaat cgccgggatc gaggtgcagc agcccgaaac caacctggtt
    17341 ttcttcaacc ccgacggcgc cggcgtcacc ggcgacgaga tggtcgaagc cttgcgcaaa
    17401 cgcggcgttc ggctcgcgat gatggacgga cgcattcgcg cctgcaccca tctcgacgtc
    17461 agcgctgcga tgatcgagga gacgataggg ctggtgcgtg agaccgtgcg ggggcgtgag
    17521 cctcacctcc ccgtcgccta gggctttgtg atcccgagcc gacctcgcgg ctgtcgtggc
    17581 agatgtgact tccgcaactg cggcggtgcg cgcctgccac agcgcgctgc cgagcaggaa
    17641 atacaccgcg ccggtccaca tcgcctgcca gttgtcggcc ccttcgttga agatgatata
    17701 gaccgcggct acggcgaaca gtcccgtgaa cacggcttcc gagaggggcc gcgtgccgga
    17761 ttttggacgg ttgaggaatg ccaatgtcca aaacggcacc acagccatcg taagggcggc
    17821 gaacgggaaa tcacgccaac gcgcgtcgaa cacgaggctt aacgcgattt gcgcggcaat
    17881 gaccgtcgtc acgatcagcg cgatgcccaa catgttcgtc atgaacaatg gagtgcgggc
    17941 tttgggtggc cccaatactt caagaaacgc cggaagcgca cgccccgaca tcaaagcgtt
    18001 ggtggacagc agcggcgcag ctattcccgc gaccagcagc aggctctgga ccagccaacc
    18061 accaaaccca tacccctcgt agaacaccct ttcagcgctt attccaaaca gaatgccaac
    18121 gatggtcgcg gataccgaga ccgcaatcca tgccgccaat cgcggcgacg actgccggcg
    18181 cttcagcgtc aacaaggctg ttccgaatac gcagatacaa agaaccagcc cgcacgccat
    18241 ctgcagcttc cagaacgggt agttgctgat ggcagcgccg gccggatatt tcagcttgcg
    18301 ttcggcccca tcgaacaagc cccagtaacc gccgaccgtt ccttcccacc cgcgcttcca
    18361 cggctcgtca taagcctcga acagattgac gcgaaagttg tcctgtctcg cccgctcgag
    18421 aagctccgat acaaaacgtg cctgattgat ccgagatggc aaagcgacgt cgcgcatccg
    18481 cccgtgactc ggccaaccgg cttcgccgat caggatctcc ttgccgggaa aggcagcggc
    18541 tacctgcttg cgaatgtcat ccacgtgcgc ggccgcatct tcggcacggg gcggaaaatc
    18601 ctcccaatag ggcaggaaat gaacggtgac gaaatcgacg tcggcgccga cctcccggta
    18661 gcgcaaccag aattcccaga cgtcggcgta ggtcaccggc acatcaacgc gcggtttaac
    18721 cgagcggatg atttcccgaa ggtcggatac gatcatctct ccccgcagca gcacttcgct
    18781 gccgacgatg atcgctgaga tcacgccggg atgatccttg gtcagcgaga ccgcgatgtc
    18841 ggtgaggaga gcgttcttcg cgcgatcgcg tccgatccag accccgagca gaaccttcaa
    18901 tccgaccctg gatgcaagct cgggcacctt gtcgaggccg ttgtcgatgg aataggtgcg
    18961 gatgcatttg gataccttgg caagctcgcg cagatcctcc gcgatctgct ccggactgac
    19021 gatgagtgcc tggtcaagcg gcgtctgatc gttgcggaac ggcgcgtagg aaacgcactc
    19081 cagcttcgcc acggcatcga taggcgcgcg cgtcagcgtc accggtgtgg ccaaccacca
    19141 ccacaccgcg gcaatgacac tcagcgatag aaagagaagc gccagcggcg tgcgaaagcc
    19201 gatcggtcct gctcccagat aggccaatag atcagcctgt ttagctgtgc gcggcaggcg
    19261 tgtcgaccca ctgagggtgc acaattgcgc cggactacgg aagcggtgat attaagctgc
    19321 ttagagcaag gtgcttgttt ctctcagaat tgatcccgcc acggcgagtg aaatgatgcc
    19381 attcaaaaga tcttccagaa gattttctcg ccgccaattt gccaggattg caggctggtc
    19441 ggctcttgga atgtcgatgc tgtcagccgg gtccagtctg gctgaattgg accaagagaa
    19501 cagaacacag aatcgaacag ccgcctcggg ttttccgaat ggttttttgt ggggtacggc
    19561 tacgtccgcc taccagatcg agggggccgt caacgaagac ggtcgcggcc catcgatttg
    19621 ggatcgattc gcccacacgc gcgggacaat ctccgatcac tgcaacgcag acaccgcgac
    19681 cgaccattat catctgtaca aggaagacgt tcagttgatg aaggccctgg gggcaaaggc
    19741 ctatcgattt tcgattgcat ggcctcgtgt cttcccggaa ggagccggtt cgccaaatcc
    19801 aaaaggcctc gatttttaca acaggctgct ggatgagttg tcggcgaacg gcatggagcc
    19861 gtttgcaacc ctgtatcatt gggacctgcc gcagacactt caggatcgct tcggcggctg
    19921 gacttcccgt gacacctcaa aggcatttgc ggattacgca ggccacgtcg cggggcgttt
    19981 gagcgaccgc gtgaagcatt ttttcacgat caacgaatgc tccaggctcg ttcatctcgg
    20041 ccacgggctt ggcgccgatg cgccgggcct caaactatcc caatcaggac tgaaccaggt
    20101 ccgtcacaac gtagcgctgg gacatggtct cgcggttcaa gccatccggg cccatggaag
    20161 cactgggacc agggtcggtc cggccgagaa tgccgtgagc tgtatccctg ctatcgaaac
    20221 gccggcgaac atccgcgcaa ccgagatggc gacgcgcgag ctcaatgccg gctatttgac
    20281 gctgatgctc gagggaaaat acacggatgc atatctcgcg caggctggca aagatgctcc
    20341 gaaatacacg gccgaagatc tgcgtgtcat ctcatcgccg atcgattttg tcggactgaa
    20401 tgtttacaca cctgaccatt atgtcattgc cgccgacaac gagcgtggct tcacactggc
    20461 gccgtttccc gcgtcgtttc cacacatgaa ggccgcctgg ctcaggcccg gccccgaggc
    20521 aatgtactgg gtgccgcgcc atgtggccaa gctgtggaac gtgaagtcga tctacatcac
    20581 cgaaaacgga acgtcgggga gcgaccaacc ggccgcggac ggaaccatct atgacgtcga
    20641 tcgcatcatg tatctgcgca accatttgac gcagttgcag cgcgcgacat cagagggtgt
    20701 gcccgtactt ggatacttcc tgtggagtct gatggacaat ttcgaatggt cggatggatt
    20761 tgagcaacgc ttcggcctct atcacgtcga cttcgctacg aaacgtcgca cacccaagct
    20821 gagcgcgtcg ttctatcgcg agacgatcgc acgaaacgcc gttgtctaat caggcgattg
    20881 tcaatccttg cgcaggccct tgcccagcgc ttcaaacatc atcgtcttaa gcgccagttg
    20941 gatccgcccg ccttgcgtcg gggcctgtac catgcagaca acgagcatgt cgtcctgggg
    21001 atcgacaaaa tagaaactgc cgcccgcgcc gtcccagcgg tattcgccga gcggccatgt
    21061 cgtattcggc ggcggtgagg tgcgcacggc aaagccaaga ccgaagccgc tggtcgggcc
    21121 cgggaaataa aagggatcgt gaatgatctt ggtctctggc ccgatctgat ccgacgtcat
    21181 cagcgcgaca gtctcgggct tgaggtaacg cttgccgtcc agcttgcctc cattcagcag
    21241 catttgcagg aagcgggcgt agtccccgat cgtcgagacc aggcccgctc caccggattc
    21301 ccagcgccgc ggcaatatcg ggtccttgat tccggctacc cgaagacgat cgatgggaaa
    21361 tggctgggca atgcgcggcc attttgcttc atctgcgaca tagtatgcgg tctcggacat
    21421 gccgagagga tcgaacaatc tttgcttttc gaattgaaat agcgtctgcc ccgaggccac
    21481 ctcgacgaca cggccgagca cgtcggttga atggctgtaa ttccaacgcg ttgcgggctg
    21541 atcggcgagc ggcagtgtcg cgatccggtc ggcgaattcg gcgttatcga aatcgccggc
    21601 gtacagttgg ggattggcat agagtttctg caccgcggtt tcgccgaaga aaccgtaagt
    21661 gatacccgac gtgtgccgca gcaggtccct gatcgtgatc gggcgcttca acggctcgag
    21721 cttgagcgga tactgtcctg cttcatcgga aagatcgacg ccgacctttg catccgcaaa
    21781 ggcacgaatg tatttcgaca cgggatcatt aagggccagc ttgccgtcgt cgaccagcat
    21841 catcgctgca accgaggtga cggccttcga catcgaatag atctggaaaa tcgtatccga
    21901 tgtcatcggc tgcccggtcg ccgggtcccg cacgccgaaa ctttgcaaat agaccggctt
    21961 gccgtgttgc tggatcagca ggactgcgcc gggaatcttg ctggtcgtga cttcattgcg
    22021 aatgtagttg ccgatctgtt caagggccgt gcgtgagaag gtcggcgcgt tcggctcggc
    22081 tcgcacctcg tgcagccatc cagcggccag gagaatggcc agcgccgcta caacaaggca
    22141 gcgcgctgcg gcgccgttag ttctccagcg cttcatagac tagctgcttc agcgtccgct
    22201 ggacgcgctg gcgctccgtc ggcgtctgct caagcaccac gaagaacatg tcctgcttgc
    22261 ggtcgatcac caaatagcag ccagcggcgc cgtcccattt cagttcgccc agcgatcccg
    22321 gcggcggcgg cttggcgtta ccgggatcgg tgcgcacggc aaatccgagc cccatgccga
    22381 agccgtcacc aggaaaatag taatgatccc gcgcaacacc ggagcccttg ccggcatggt
    22441 ccgatgtcat cagttcgaac gttttcgggc tgagataggt cctgccctcg aacgtaccgc
    22501 cattggtcag catctgcgca aagcgcgcaa agtccgacat ggttgaaacc atgccgccgc
    22561 tggcggattc ccacttctgg cgaacctcgg tcctgtattc ccggccgacc cgaaagttac
    22621 tgtcgttcgg cagcggctgg gcgagccgct gcagcctctc cggttcggtg acgaagaagc
    22681 cggtgtcggt catgccgagc ggatcgagca gctttccccg ctcgatctcg agcaatgatt
    22741 ttccggacac gatctccatg atccgcgcca ggatgtcggt ggaatggccg tattgccaga
    22801 gcgctcccgg ctggttgtgc agcggcagct tggcgatccg ctcagcgaat tcggccaggt
    22861 cgaattcgcc cgcatagaga tttgccgccg cgtaagcctt gcgcaccaga ctgtcgccat
    22921 agaagccata ggtgatgccg gaggtatgac gcatcaggtc gagaatggta ataggccggg
    22981 tcagcgtcac cagttcgagc gtcttggtac cgtcctcggc ctttttctcc tcaccgacct
    23041 tcatatttgc gaaggaggga atgtacttcg agacgggatc gtcgagcttg atcttgccgt
    23101 cctggatcaa ctgcatcgcc acaacagagg tgatcgcctt ggtcagcgaa gacaggcgga
    23161 agatggtttt gtccgtgatc ggcgcctgcg tcaccacatc ctgcacgccg aaggattggt
    23221 gatagaccgg cttgccgtgc tgctggatga gcaggatcgc gcccgagatc ttgcccgtcg
    23281 taacttcgtt gcggaagaac tcgccaatcg tatcaagctt ttgcgggttg aaatgcgcgc
    23341 ccgcgggaat atcgaaggta ccttccgcca tggccgacgg cgctgccgcc agcgtcagca
    23401 gcgcaccaca aatcagcgcg cgcaacacaa cgcctgaatt catcgtcccc tccccaaaat
    23461 cacctgccgc ggagtgtagc ggccggctca atcggcacaa gggctgccca attgcgccat
    23521 cgcctgcgca tgcagccggg cgctgtcgtc ggagaagcag cagaagatga tgcgggtgat
    23581 cacgggcgcc gctgcaagcg cttccgcggt cgccgagacc gcgattcccg cggcgcggcc
    23641 ggcggggaaa cgatagacgc cggtggagat cgccggaaag gccatcgaag cgaggccatg
    23701 ggtctggccg agctcgatgg cgcggcgata gcatgacgcc aaggcctcgt cctcgctttg
    23761 gctcccgccg tgccagaccg gaccgacggc atgaatcaca tgccgcgccg gcaaccgata
    23821 accgctggtg attttggcgt cgccggtttc gcaaccgccg agcgcgcggc attcggcgag
    23881 aagccccggt cccgcggcgc ggtggattgc accatcgacg ccgccgccgc cgagcagcga
    23941 ggtattcgcc gcgttcacga tcgcgtcaac gccgagcgtc gtgatgtcgc cgacgataac
    24001 ttccagccgc gccgttccga tcaggcgcgc gccctggctc aggcggaggc cgccgcgctg
    24061 accgtgacgc ccttgtcggc aagcagctgc tgcagctcgc cggcctggaa catctcgcgg
    24121 atgatgtcgc agccgccgac gaattcaccc ttgacgtaga gctgcggaat ggtcggccag
    24181 ttggaatagg tcttgatgcc gttgcgcaga tcggcggact caaggacatt caggcccttg
    24241 tagccgaccc cgacgtgatc gaggatctgc accacctggc cggaaaaacc gcactgcgga
    24301 aactgcggcg tccccttcat gaacagcacg acgtcgttcg acttcacttc gttgtcgatg
    24361 aattgctcga tgctcatatt cgcgtccttt tccggcacgg cccctgggcc ggcgtcagct
    24421 ccttaacccg acattgtcat atatgtaccc gaaaccattg tgcatcaaaa gcaaaatggc
    24481 gaggagctgg catgcatggg tctgcccgcc ccggaaatcg caaggaccgg gcccgggcca
    24541 tagcctcgaa cggggccttg ggagcctttt atccccggcg gaagccccta tgtaggacag
    24601 gctattctcg gggaacccgt ctgccatcgt agcgttctcc cccgaacgga gagtattgtg
    24661 acgaaaccag catccgccgc ggccacgctt gcggcccctt gcccgtcatt attctccgat
    24721 tccggcagcc tcgccgagga tctggtggag gcgtatctca cagtccgaaa cgagaccgag
    24781 tgccgcgccg accccctgac gccagaggat cagctgatcc agtcgatgcc ggatgcgagc
    24841 ccggccaaat ggcaccgcgc ccataccacc tggttcttcg agcagttcct gctcggcgag
    24901 cattgcaggg gctacaagcc ctttcatccg gattatgcgt atctgttcaa ttcctactat
    24961 gtcagcgccg gaccacgcca cgcccgccac cagcggggcc atctgacccg ccctggcgcc
    25021 gacgaaatca ccgcctaccg ccggcacgtc gatgccgccg tggtgaagtt tttccagact
    25081 gccggtgagg acgctttgac cgcgatcgcc ccgctcgtgg gagtcggcct caatcacgag
    25141 cagcagcatc aggaattgat gctgacggac atcctgcacg ccttcgccca aaacccgata
    25201 ccgccggctt acgatccggc gtggagtttt cccgtttcaa gccgcgccgg cgacgagaag
    25261 cctcgcgccg gcgacgagaa gcctcgtgct ggcgacgaat gggtcaccct cagcgagggc
    25321 atccacaccg tcgggcacac cgacgacacg tttcatttcg acaacgaaaa acccgcgcac
    25381 cgcgcgctgg tgggacccgt gaagcttgcg cgcaacctcg tcaccaacgc cgaatggctc
    25441 gcattcatgc gggacggtgg ctacaccaca ccgacgctgt ggctgatgga cggttttgcc
    25501 gtcgtcggca acgagcagtg gcaggcgccc ggccactggc gtcaggtcga cggcgaatgg
    25561 cggatcatga ccctcggcgg attgcagccc gtcgatccgt cagccccggt ctgccatgtc
    25621 agctattacg aggccgatgc gtttgcgcgc tggagcggca ggcatttgcc gaccgaaatg
    25681 gaatgggaag tcgccgcccg tgccggccag ctcaacgacg cattcgggat cgtctggcag
    25741 tggacccgca gcgcctacgc cccctatccc ggttaccgcg ccatcgaggg cgcgctcggc
    25801 gagtacaacg gcaagttcat ggtcaaccag ttggtgctgc gcggctcctc gcttgcaact
    25861 cccgacggac atagccgtat tacctatcgc aacttctttt atccgcacca tcgctggcaa
    25921 ttcacgggac tgcgcctcgc cgactatgcc atctgatttt ttcaccaatt catcgcgccg
    25981 gatagcgcgt tcaggagagt atcatgaatg tgcacgccgc agctttggcc aaaacccatc
    26041 gtttcgacga acagacctcc gcatttgcca gcgacgtcat cgacggtcta tcgcagcacc
    26101 ccaaacggct gtcgccaaaa tatttctacg acgccacggg ctcggagctg ttcgaacaga
    26161 tcacgctgct gccggaatat tacccgacgc gcaccgaatt gacgattctg cgcgaccgcg
    26221 ccagcgagat atcggccgtc gtcccgaaag gcgccgcact ggtggaattc ggcgccggcg
    26281 cgaccaccaa ggtccggctg ctgctgaacc aatgcgcgtt tggcgcctat gtgccggtgg
    26341 atatttccgg tgacttcctc aaagcccagg ccgacgcctt gcgcaaggat tttcccgcgc
    26401 tcgatgtcta tccggtcgcg gcggacttta ccgcgccgtt cgccctgccc gacgcggtgc
    26461 aggcgatgcc gaggctcgga tttttcccgg gatcgacgct cggaaatttc gagccgcacg
    26521 aagcctgcgc cttcatgcgc agcgcccgcg acatcctcgg cgaggccgcg cagatgatca
    26581 tcggcgtcga cctcgaaaag aacgagcggg tgctgtataa tgcctacaac gacgccgccg
    26641 gcgtcaccgc acgcttcaat ctcaacgtgc tggtccgcat caaccgcgag ctcggcggca
    26701 atttcgacac cgccggattt acccatcgcg cgatctacaa tcgcgaccgt caccgcatcg
    26761 agatgcacct gatcagccgc aagacccaga ccgtgcgcgc gttggggcgg agtttttcgt
    26821 tccgcgccgg cgagagcatc cacaccgaga gcagctataa atacagcctc gagcgtttta
    26881 cggcgctggc gcgcggctcg ggctggagct cgcgccactc gtggaccgac aaggccggca
    26941 tgttttcggt tcacgcgctg gtggcttcgg actgagattc tgcggtcgga gaccccacgg
    27001 tccgggattc ggccgggccc gatttcagcg agatcgattc ccacacggcg acaatgatca
    27061 tgatcgcggt ggtggcgatc gacagcagca acggcgatag ctcgccggcg aaccaggcca
    27121 gcccgccaag ggccacgatg ccgacgccat gcgacagctg aaggaagccg cgaatggtgt
    27181 gtttgaagag aatggttccg agcagaaaca gcagcggtcc gccgatcgca gtgaacaccg
    27241 tcctcggatc ggaatgatcc gccggatgcg tcagcaccag ttcatcggcg accgccgaaa
    27301 gaatgatgcc ggccacgatc ggcatatgca gataggtgta ggccagcctc gccagccgcc
    27361 cgggctcgct ggattttgaa atcagttcgg accccgcttc gacgcccttg tggaaataga
    27421 tccaccacat tgcgagactg ccgataaagg ccgacacaaa ggcgaccacg gtctccgtgg
    27481 tccaggccag atcggcgaac gtcgcgccgg tcacgacaat ggattcgccg agcgcgataa
    27541 tgatgaatcc ggcgcagcgc tccgccatat ggccgccttc aaccatccag tcctcgatcg
    27601 aagacgcgcc gtatttcggg atccagaacc ggaccgccgg cgagagatat tcgatgccca
    27661 gcgcgatggc ccaaagggcc agccgccaat tcccttccgc aaagcctccc gcgatccaaa
    27721 agatcgccga catggctagc caggcggtga tacggaccgc attcaagcgc gccagatttc
    27781 ggctaggcgg tgtcgatacc cacagaaaga ccgtcttgcc gacctgcatc gcggcgtagg
    27841 cggtggcgaa ccacagtccc cgcgattcaa aggcttttgg aatcgaggtc gagagcacga
    27901 gcccgccaag catcagcaaa aacagcagga tgcggactgg cgtgacttcc ggattgagcc
    27961 agttggtgat ccatgaggtg tagacccaca cccaccagac ggccaggaac aacaggctcg
    28021 tctgcaatgc ccctagcgga gtgaaccggc cgatc
//
LOCUS       DATNOI010000499        10457 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_26460,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000499 DATNOI010000000
VERSION     DATNOI010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10457
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26460"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..396
                     /locus_tag="VN968_20145"
     CDS             118..396
                     /locus_tag="VN968_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HXL31581.1"
                     /translation="MNRTDCSTSSRWAIILLSAAVLALGGCGRKGGLDLPPNAASAQA
                     TAAEQADAEAERAASKPSVFNPTYGMDAGPAAPKGRKKPFVLDPLLND"
     gene            404..1669
                     /gene="lysA"
                     /locus_tag="VN968_20150"
     CDS             404..1669
                     /gene="lysA"
                     /locus_tag="VN968_20150"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HXL31582.1"
                     /translation="MNHFDYRNGVLHAEAVDLSKLADAVGTPFYCYSTATLERHYRVF
                     SEAFADQKALVCYAMKANSNQSVLRTLAKLGAGADVVSGGELKRALAAGIPPGKILFS
                     GIGKTEAELRAALAEGILCINVESEPELELLSRLASETGRTARISVRVNPDVDSGSHA
                     KISTGKSENKFGVPLDRARAVYARAAKLPGIKVTGVDMHIGSQITDLGPLEAAFMLLV
                     DFAQALRADGHSISHIDFGGGLGIPYHPETPTPPEPAAYAAVVKRAMRGLDCTLMFEP
                     GRMIVGNAGILVTRVIYVKHGDAKNFVIIDAAMNDLIRPTLYEAYHDLLPVRLRKPEA
                     GKIVADVVGPVCESGDYLALGRTLPEPRSGDLLAIMTAGAYGAVQSSSYNTRALVPEV
                     LVKDDQYAVVRPRIEVDALIAMDRPAPWL"
     gene            complement(1760..1885)
                     /locus_tag="VN968_20155"
     CDS             complement(1760..1885)
                     /locus_tag="VN968_20155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31583.1"
                     /translation="MLRWALCPLDMYADSDFTSTAKPSDGRALDAAQRNGGVVRC"
     gene            complement(1903..2811)
                     /locus_tag="VN968_20160"
     CDS             complement(1903..2811)
                     /locus_tag="VN968_20160"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL31584.1"
                     /translation="MDIGFIGLGKMGFPMARRLIEANHQLVVFDQRQEAVDKLVALGA
                     QAASSPKDVADRAETVLASLPSLQASLEVATGSGSVIEGKRVRRFVDLSTVGSHMAVR
                     IHDLLAKRNIVQLDSPVSGGVGGAEKGTLAVMVSGSKADFELIKPALEVIGKVFFIGE
                     KPGSAQTMKLANNLLSATAVAATSEAVVMGVKSGLDPHVMIDVINAGSGMNTASRDKF
                     PRAILPRTFDFGFATGLMVKDVRLCLEEAKSLGLSMEVAEAVGRLWEVVIREMGAESD
                     FTSAIKPIEQAAGVVVGGANGAHAAK"
     gene            complement(2869..3249)
                     /locus_tag="VN968_20165"
     CDS             complement(2869..3249)
                     /locus_tag="VN968_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXL31585.1"
                     /translation="MDKKTHDKGLEIRKAVLGEAYVSNALKNADDFNKPFQELVTEYC
                     WGAVWGREELPRKTRSMLNLAMISILNRPHELRVHIKGALVNGVTKDEIREIFMQVAI
                     YAGVPAGVDSFRIAREVFAEIDKS"
     gene            3412..6000
                     /locus_tag="VN968_20170"
     CDS             3412..6000
                     /locus_tag="VN968_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HXL31586.1"
                     /translation="MSGTTPDPSEPARDADASSRLQLAQALQRAKYAIAWERSWPHFA
                     RLLTVAGLFLAVSWAGLWLALPFVARAIGLGVFVLLALGALFPLVRFRWPSREAGLSR
                     LDRGTGIRHRPATALTDTLSTQDPVALALWQAQRERTLASIKRIRAGLPSPRLVIHDP
                     WALRALVAVLMVAAYIAAGDERRLRTEAAFDWNGVLSPANIRVDAWVTPPLYTGKPPI
                     ILSAANKEAAMPAAGPLSVPAGSTLIVRSSGGTLDVVAAGNVTEAVPTEQAPKGTNER
                     HFTISGDGTAHVRAPSGQPQWKFSAIPDRAPTIALAKDPERQARGSLQMSYKIEDDYG
                     VTEAQAHFAVRPGDAPKAGAEPRPLFAAPQFSLVLPNARTRNGVGQTVKDLSEDPYAG
                     ADVTLTLTAKDEAGNEGRSEPFNMRLPERLFTKPLARALIEQRRILALDANQNTPVYT
                     ALDALMIAPELFMPETGQYLGLYSIARQLEAARTDDAMREVVAGLWALAVSIEDGDIT
                     DVDKALRAAQDALKQALERGASDEEIKKLTENLRAALDNFIRQLVEQQRNNPQQLARP
                     LDPNTKMLSQQDLKNMIDRMERLSRSGDKDAAKQLLDQLQQMLENLQMAQPGQSGDSE
                     MEQALNELGDMIRKQQQLRDKTFKQGQDSRRDRMRGKQGDQSMSDLQQDQQGLRDRLK
                     RLQEELAKRGMGPGQRGDKGQRGEKGQRGEQGQPGQEGQQGDQGDGEDGLGEADSAMD
                     DATGRLGEGNADGAVDSQGRALEALRKGAQSLAEAMQQGDGEGQGDSPGNRAGRQQSG
                     ANTNDPLGRPLRGREYGDDLTVKIPGEIDVQRVRRILEELRRRLADPQRPQIELDYIE
                     RLLKDY"
     gene            complement(6166..6543)
                     /locus_tag="VN968_20175"
     CDS             complement(6166..6543)
                     /locus_tag="VN968_20175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL31587.1"
                     /translation="MPRVLIADDEDSMRTLVARAVAMDGHDTVTAEDGAEALEILIRE
                     RGAFDLLLTDIQMPVMDGIALALAAARDFPDLTILLMTGFAAQRERAHGLNAIVHDVI
                     TKPFSVADIRTAVADALAAGKKG"
     gene            complement(6584..7420)
                     /locus_tag="VN968_20180"
     CDS             complement(6584..7420)
                     /locus_tag="VN968_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HXL31588.1"
                     /translation="MHIICPHCTTSYAIDLATLGASGRTVRCSRCKEVWLARPEDATA
                     MAAPVPAMAEAGQTSAADAAAEWDALVREDEETPVVDSPSISADWPADGQGLPQDSEN
                     DWPSIARQDLSDPETGRPQRLSWLRRLAKPLVSLPTVCAAMGALVLGLMIWRADVVRL
                     LPQTATFYKLAGLGVNLRGLVFKDIKITTETMEGKPVLVIEGAIVGEARKPVELPRLR
                     FSVRDAQGAEIYAWNAVLEQLVLKPGETAWFKSRLASPPPEGRNIDVRFFGKRDVAAG
                     DT"
     gene            7595..8254
                     /gene="ftsE"
                     /locus_tag="VN968_20185"
     CDS             7595..8254
                     /gene="ftsE"
                     /locus_tag="VN968_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HXL31589.1"
                     /translation="MVRFENVGLRYGLGPEILRDLSFQIPAHSFQFLTGPSGAGKTSL
                     LRLLFLSLRPTRGLVNLFGHDVSMLGKDDVADLRKRIGIVLQDFRLLDHMTTYENVAL
                     PFRVMGREESSYRKEVIDLLKWVGLGERMDALPPVLSGGEKQRAAIARAVISRPLLLL
                     ADEPTGNVDPTLGRRLLRLFIELNKSGTAVIIATHDITLMDQYEARRMVLHHGRLHIY
                     E"
     gene            8247..9218
                     /locus_tag="VN968_20190"
     CDS             8247..9218
                     /locus_tag="VN968_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31590.1"
                     /translation="MNRMGDERGLLVDLGQERPRVSASARNLSPIVPRGSIAGRALVA
                     VVAIMTFLASMTTGAVLLISASAAEWQSEVASEITIQVRPLPGRDIERDTLAVAEAIR
                     VQPGIVDVRPFTKDQSAKLLEPWLGTGLSLDDLPVPRVVVARVQPGTTLDLAALRSRV
                     TKAAPSASVDDHRAWIERMRSMTGATVLAGIGILALVIIATIISVSFATRGAMAANRP
                     IVEVLHFVGAGDRYIANRFLRHFLRLGLQGGVIGGGVAMFTFGFSESIAGWFSGTPIG
                     DQFGALLGTFALRPSGYLALAAQAALIAAITAWASRRTLFATLDDID"
     gene            9409..10128
                     /locus_tag="VN968_20195"
     CDS             9409..10128
                     /locus_tag="VN968_20195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HXL31591.1"
                     /translation="MSLQPDDKSPNAPSSARRGWLRATIVAVSAIAFVTAAIGFVGFL
                     SKLRGAELKPARNADGIVVLTGGSSRVSDAMELLAGGYGKRLLISGVHPTNAASDISR
                     SLPDNQSLLSCCVDLDRSAVNTRSNAAQTRRWARDRGFKSLIVVTSNYHMPRAIVELS
                     HAMPDVALIPFAVVGDKWRDEPWWTNGATLRLLLSEYVKYVAAEVRVRLADAGLELTP
                     ELADQPSGSLALPRPATAQAN"
     gene            10140..>10457
                     /locus_tag="VN968_20200"
     CDS             10140..>10457
                     /locus_tag="VN968_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HXL31592.1"
                     /translation="MVSIFLRSLVYNVLFYALLVFWILIAIPTYLMPPRAFMTVAKAW
                     ARSSVWLMRVICNTKVEYRGLEKIPKGPLIVASKHQSMWETFALLQFFDAPLFIYKRE
                     LGWI"
BASE COUNT         1809 a         3301 c         3450 g         1897 t
ORIGIN      
        1 ctggaaaaag agcgaaaatt gggctgagct cacgaaattc gctgcgattc atgttggcct
       61 gggtctcgcc agaacagggc aatctttgta tggtgccgcc gcattgggga ttttgccgtg
      121 aatcgtactg actgttcgac atcttcgcga tgggccatca tcctgctcag cgcggcggtg
      181 ctcgcgcttg gcggctgcgg acgcaaaggc gggctcgacc tgccgccgaa cgccgcctcg
      241 gcgcaggcga ccgccgccga gcaggccgat gcggaggccg agcgggccgc gagcaaaccg
      301 agcgtgttca atccgaccta cggaatggat gcgggaccgg ccgcccccaa gggccgcaag
      361 aaaccattcg ttctcgatcc gctcctgaac gactgagcct gccatgaatc acttcgatta
      421 tcgtaacggc gtgcttcacg ccgaagcggt tgatctgtcc aaactggcgg atgcggtcgg
      481 cacgccgttc tattgctatt cgaccgcgac cctggagcgg cattaccggg tgtttagcga
      541 ggcctttgcc gatcagaagg cgctggtgtg ttacgccatg aaggccaact ccaatcaatc
      601 ggtgctgcgc accttggcga agctcggcgc cggagccgac gtggtgtcgg gtggcgagtt
      661 gaagcgcgcg ctggcggccg gcattccgcc gggcaagatc ctgttctcgg ggatcggcaa
      721 gaccgaggct gagctgcgcg ccgcgctggc cgagggcatt ctctgtatca acgtcgaatc
      781 cgagcccgaa ctcgaactgc tctcgcggtt ggccagcgaa accgggcgga cggcgcggat
      841 ttcggttcgg gtcaatcccg atgtcgattc ggggtcgcat gcgaagattt cgaccggaaa
      901 atccgagaac aagttcggcg tgccgctcga tcgcgcccgc gcggtgtatg cccgcgcggc
      961 aaaactgccg ggaatcaagg tcaccggcgt cgacatgcat atcggcagcc agatcaccga
     1021 tctcggtccg ctggaagccg ccttcatgct gctggtggac ttcgcgcagg cgttgcgcgc
     1081 cgatggccac agcatttcgc atatcgattt cggcggcggg ttgggtatcc cctatcatcc
     1141 ggagacgccg acgccgcccg agccggccgc ctatgccgct gtcgtcaagc gggcgatgcg
     1201 cggcctcgat tgcacgctga tgttcgagcc cggacggatg atcgtcggca atgccggcat
     1261 cctggtgacg cgcgtgatct atgtgaagca tggcgacgcg aagaatttcg tgatcatcga
     1321 cgcggcgatg aacgacctga tccgtcccac tctgtacgag gcctatcacg acctgctgcc
     1381 ggtgcggctg cgcaagccgg aagccggcaa aatcgtcgca gacgtggtcg ggccggtgtg
     1441 cgaaagcggc gattatctcg cgctcgggcg caccctgcca gagcccagat ccggcgatct
     1501 cctggcgatc atgaccgccg gcgcctacgg cgcggtgcaa tcctcaagct acaacacccg
     1561 cgcgctggtg ccggaagttc tggtcaagga cgatcaatac gcggtggtgc ggccccgcat
     1621 cgaggtcgat gccctgatcg cgatggatag accggcgccg tggctgtgaa tacctcgtgt
     1681 cccggacgcg gtgcagcgca cttggcgctg ctccgcagag ccgggaccca gatgcagcga
     1741 tgcaagatgg gccccggatt cagcagcgca ccacgccgcc attgcgctgc gcagcatcca
     1801 gggcacgtcc atcgcttggc ttggccgtcg aggtgaaatc ggaatccgcg tacatgtcga
     1861 gaggacagag tgcccagcgc agcatccggc gctcgcgccc cttcacttgg cggcgtgagc
     1921 gccgtttgcc ccgcccacca ccacacccgc cgcctgctcg atcggcttga tcgccgaggt
     1981 gaaatcggac tccgcgccca tctcgcgaat caccacttcc cataaccgtc caaccgcttc
     2041 ggcgacctcc atcgatagcc ccagcgactt ggcttcttca aggcagagcc ggacgtcctt
     2101 caccatcagc ccggtggcaa agccgaagtc gaaggtgcgc ggcaggatcg cgcggggaaa
     2161 cttgtcgcgg ctcgccgtgt tcatgcccga tcccgcattg atgacgtcga tcatgacatg
     2221 gggatcgagg cccgatttga cgcccatcac cacggcctcg gaggtggcgg cgacggcggt
     2281 ggccgacaac agattgttgg cgagcttcat ggtctgcgcc gagccgggtt tctcgccgat
     2341 gaaaaacacc ttgccgatca cctcgagcgc gggttttatc agttcgaaat ccgctttcga
     2401 gcccgacacc atcacggcga gcgtgccctt ttcggcgccg ccgaccccgc cactgacggg
     2461 actgtcgagt tgcacgatgt tgcgtttcgc cagcaaatcg tgaatccgca ccgccatgtg
     2521 cgatccgacg gtggagagat cgacgaagcg cctgacgcgc ttgccttcga tcacgctgcc
     2581 gctgccggtt gcgacctcga gcgaggcctg cagcgacggc aggctcgcca gcacggtctc
     2641 ggcgcggtcg gcgacgtctt tcggtgacga tgcggcctgc gcgcccagcg cgaccagctt
     2701 gtccaccgcc tcctggcgct ggtcgaagac cacgagctga tggtttgcct cgatcaggcg
     2761 gcgggccatg ggaaagccca ttttcccaag gccgatgaat ccgatatcca tgatgatacg
     2821 cctttagccc aagtcgattt agcccaagtc gagttagctc ttgtcgattt agctcttgtc
     2881 gatttcagcg aacacttcgc gcgcaatccg aaaactgtcg acgcccgcgg gcacgccggc
     2941 atagatcgcc acctgcatga agatttcgcg gatctcgtcc ttggtcacgc cgttgaccag
     3001 cgcgcccttg atgtgcaccc gcaattcatg cggccggttg aggatcgaga tcatcgccag
     3061 attgagcatg ctgcgggtct tgcgcggcaa ttcctcccgg ccccacaccg cgccccagca
     3121 atattcggtg accagttcct ggaatggctt gttgaaatcg tcggcattct tcagggcgtt
     3181 cgaaacatag gcctcaccga gcaccgcttt gcgtatttcc agtcccttat cgtgggtttt
     3241 cttgtccata gcgcttccct ctggtgtgat ggcaccggac gttacgaggt cggggcgctg
     3301 gcgtcacgcc ttcgtgttat gtgcgatttg aggtgtgggt tgtgcattgc gggacgtgcc
     3361 tcattgccgc cgttgtgcta gagtgctgtt tcgggcaacc aggagagttg attgagcggc
     3421 accacccccg acccctcaga gccagcgcgc gacgctgatg cttcctccag gctgcagctg
     3481 gcgcaggcct tgcagcgagc aaaatacgcg attgcgtggg agcgaagctg gccgcatttc
     3541 gcgcgcttgt tgacggtcgc ggggttgttt ctggcggtat cctgggccgg gttgtggctg
     3601 gcgctgccgt ttgtcgcgcg cgccatcggc cttggggtgt tcgtgctttt ggcactcggg
     3661 gcgctgtttc cgctggtccg gttccgctgg cccagccgcg aagcggggct gagccggctg
     3721 gaccgcggta ccggcatccg ccatcgcccg gcgaccgcgc tcaccgatac gctgtcaaca
     3781 caggatccgg tcgcgctggc gctgtggcag gcgcagcgcg agcgcactct ggcgtcgatc
     3841 aagcgaatcc gcgccggtct gccgtcgccg cggcttgtga tccacgatcc ctgggcgttg
     3901 cgcgcgctgg tcgcggtgct gatggttgca gcctatattg ccgcgggcga cgagcgcagg
     3961 ctgcgcaccg aggcggcgtt tgactggaac ggcgtgctgt cgccggccaa tatcagggtt
     4021 gacgcctggg tgacgccgcc gctctacacc ggcaagcctc cgatcattct gtcggcagcc
     4081 aacaaggaag cggcgatgcc ggccgcggga ccgttgtcgg tcccggcggg cagcacgttg
     4141 atcgtgcgct caagcggcgg tacgctcgat gtcgtcgccg ccggcaacgt gaccgaagcg
     4201 gtgccgaccg agcaggcgcc caagggcacc aacgaacggc atttcaccat atcgggcgat
     4261 ggcacggcgc atgtccgtgc accgtccggg cagccgcaat ggaagttcag cgcgattccc
     4321 gaccgcgctc cgaccattgc gcttgccaaa gatcccgagc gtcaggcccg cggctcactg
     4381 cagatgtcct acaagatcga ggatgactac ggcgtcaccg aagcgcaggc gcattttgcc
     4441 gtccggcccg gcgacgcgcc gaaagccggc gcggagccgc ggccgctgtt cgcggcgccg
     4501 caattctcgc tggtgctgcc gaatgcgcgg acgcgcaatg gcgtcgggca gaccgtcaag
     4561 gatctcagcg aggatcccta cgccggcgcc gacgtcacct tgacgctgac ggccaaggac
     4621 gaggccggca atgagggccg cagcgagccg ttcaacatgc ggctgccgga gcggctgttc
     4681 accaagccct tggcgcgggc gctgatcgag cagcgccgca ttctggcgct cgacgccaat
     4741 caaaacacgc cggtctatac ggcgctggat gcgctgatga tcgcacccga attgttcatg
     4801 cccgaaaccg gccagtatct cgggctgtac agcatcgcga ggcagctgga ggccgcccgc
     4861 accgacgatg cgatgcgcga ggtcgtcgcc ggcctgtggg cgctggcggt cagcatcgag
     4921 gatggcgaca tcaccgacgt cgacaaggca ttgcgtgccg cgcaggacgc gctgaagcag
     4981 gcgctggaac gcggcgccag cgacgaagaa atcaagaagc tcaccgagaa tttgcgcgcg
     5041 gcgctggaca atttcatccg tcaactggtc gagcagcagc gcaacaatcc gcagcaactg
     5101 gcgcggccgc tcgatcccaa taccaagatg ttgagccagc aggacctcaa gaacatgatc
     5161 gaccgcatgg agcgcctgtc gcgctccggc gacaaggatg cggccaagca attgctcgac
     5221 cagctgcagc agatgctgga aaacctgcaa atggcgcagc ccggccagtc cggcgacagc
     5281 gagatggaac aggcgctgaa cgagctcggc gacatgatcc gcaagcagca gcagctgcgc
     5341 gacaagacct tcaaacaggg ccaggattcg cggcgcgacc gcatgcgcgg caagcagggc
     5401 gatcaaagca tgagcgatct gcagcaggac cagcagggcc tgcgcgaccg gctcaagaga
     5461 ttgcaggaag agctggccaa gcgcggcatg gggccgggcc agcgcggtga caaaggccag
     5521 cgcggtgaga aaggtcaacg cggcgagcaa ggccaaccgg gccaggaagg ccagcagggc
     5581 gaccagggcg acggtgagga cggtctcggc gaagccgatt cggcgatgga cgatgccacc
     5641 ggcaggctcg gggagggcaa cgccgatggc gctgtggatt cccagggccg ggcgctggaa
     5701 gcgctgcgca agggcgccca gagtctggcg gaggcgatgc agcagggtga cggcgaaggt
     5761 cagggtgaca gcccgggcaa ccgcgcgggg aggcaacaga gcggcgccaa caccaacgat
     5821 ccgctcgggc ggccgctgcg cgggcgcgaa tacggtgacg atctgacggt gaagattccc
     5881 ggcgagatcg atgtccagcg ggtgcgccgc attctggaag aactgcgccg ccgcctcgcc
     5941 gatccgcagc ggccccagat cgagctcgat tacatcgagc ggctgttgaa ggattattag
     6001 ggcttcgtct tcatgccggc cgtcgtcatc ggggcttgtc atacgcgggc ttgacccgcg
     6061 tatccatcat cttcgaaaga tggattgccg ggagcgcaga caagtttacg cagtctgcgc
     6121 aaggcagact actatgcccg gcaatgacgg gttgatgcat ctgcgttagc ctttcttccc
     6181 cgcggccagt gcgtcggcca cggcggtgcg gatatcggcg accgagaacg gcttggtgat
     6241 gacgtcgtgc acgatggcgt tgaggccgtg cgcgcgctcg cgctgggcgg cgaaccccgt
     6301 catcaacaga atggtcagat cggggaaatc gcgcgccgcc gccagcgcca gcgcgatccc
     6361 gtccatcacc ggcatctgga tatcggtgag caagagatcg aacgcgcccc gttcgcggat
     6421 caggatttcg agcgcttcgg cgccgtcttc cgcggtgacg gtgtcgtggc cgtccatggc
     6481 gacggcacgc gccaccagcg tgcgcatgga gtcttcatcg tcggcgatca gcacgcgcgg
     6541 catggctttc ccgtcctttg cgagcgggcc gggcgacccc cgttcatgtg tcgccggcgg
     6601 cgacatcccg cttgccgaag aagcgtacat caatattgcg cccttcgggc ggtggcgagg
     6661 ccagccgcga cttgaaccag gctgtttcgc ccggcttcag caccaattgc tccagcaccg
     6721 cgttccaggc atagatctcg gcgccctggg cgtcgcgcac ggaaaagcgc agcctcggca
     6781 attcgaccgg cttgcgggct tcgccgacga tggcgccttc gatcaccagc accggtttac
     6841 cttccatcgt ctcggtggtg atcttgatgt ccttgaacac aagcccgcgc agattcaccc
     6901 cgaggccggc cagtttgtag aatgtcgcgg tttgtggcag cagccgcacc acgtcggcgc
     6961 gccagatcat cagccccagc accagcgcgc ccattgcggc gcagaccgta ggcaggctga
     7021 ccagcggttt tgcaagcctg cggagccacg acaggcgttg cgggcgaccg gtctcggggt
     7081 cggacaggtc ttgccgggct atcgacggcc agtcgttttc gctatcctgg gggaggcctt
     7141 ggccgtcggc cggccagtcg gcggatatcg aggggctgtc gacgaccggg gtttcctcgt
     7201 cttcgcgcac aagcgcatcc cactccgcgg cggcgtctgc ggctgaggtc tgcccggctt
     7261 ccgccatcgc aggcaccggc gctgccatcg cggttgcatc ctccggccgc gccagccaga
     7321 cctccttgca gcgggagcag cggacggtac ggccggatgc gcccaatgta gccaaatcaa
     7381 tggcatagga cgttgtacaa tgggggcaaa tgatgtgcat ggagccaatc gccacaatga
     7441 cgctgccgaa atgctacaac gcgaccatta aggaaacgga aaccataacc gaagcacaac
     7501 cagtttgtcg ggttcgcagc cgctgcgagc cgacgagctt gcgggccccc ggttccgggt
     7561 ctccggccat gccccacgtc gaacggagct gagcttggtt cggttcgaaa atgtcggatt
     7621 gcgctacggg ctcggcccgg agattctgcg cgacctcagt ttccagatcc ctgcccattc
     7681 cttccagttc cttaccggcc cctccggcgc cggcaagacc tcgctgctga ggctgctgtt
     7741 cttgtcgctg cggccgaccc gcggcctggt caatctgttc ggccatgacg tctcgatgct
     7801 cggcaaggat gacgtggccg atctgcgcaa gcggatcggc atcgtgctgc aggactttcg
     7861 cttgctcgac catatgacta cctatgaaaa tgtggcgctg cccttccggg tgatggggcg
     7921 cgaggaatcg agctatcgca aggaggtcat cgatctcttg aaatgggtcg ggctcggcga
     7981 gcgaatggat gcgctcccgc cggtgctgtc cggcggcgag aagcagcgcg cggcgattgc
     8041 acgcgcggtg atttcgcgcc cgctgctgtt gctggcggat gagccgaccg gtaacgtcga
     8101 tccgaccctg gggcgacgat tgctgcgctt gttcatcgaa ctgaacaagt ccgggacggc
     8161 ggtgattatc gcgacccacg acattacgct gatggatcag tatgaggcgc ggcggatggt
     8221 gctgcatcac ggacggttgc atatctatga atagaatggg tgacgagcgc ggcctgttgg
     8281 tggatttggg gcaagagcgt ccgcgggtgt cggcgagcgc gcgtaatctc tcgccgatcg
     8341 tgccgcgggg ctcgatcgcc ggccgcgcgc tggtcgcggt cgtcgccatc atgacgttcc
     8401 tcgcatcgat gaccacgggc gcggtgctgc tgatcagcgc gtcggcggcg gagtggcagt
     8461 cggaggtcgc gagcgaaatc accatccagg tgcgcccgct gcccgggcgc gatatcgaac
     8521 gcgatacttt ggcggtggcc gaagcaattc gcgtgcagcc gggcatcgtc gatgtcaggc
     8581 ctttcaccaa ggaccagtcc gccaaactgc tggagccatg gctgggcacc gggctctcgc
     8641 tcgacgatct gccggtgccg cgcgtcgtgg tggcgcgggt gcagcccgga acgacgctgg
     8701 atttggcggc gctgcgcagc cgggtgacca aggcggcgcc gtcggccagc gtcgacgatc
     8761 accgcgcctg gatcgagcgg atgcgatcga tgaccggcgc aaccgtgctt gccggcatcg
     8821 gcatcctggc gctggtgatc attgcgacca tcatttcggt atcgtttgcg acccgtggcg
     8881 cgatggcggc caaccgcccg atcgtcgagg tcctgcactt cgttggagcc ggagaccgct
     8941 acatcgccaa ccggttcctg cggcatttcc tccggctggg gctgcagggc ggcgtgatcg
     9001 gcggcggcgt cgcgatgttc actttcgggt tttccgaatc catcgccggc tggttctcgg
     9061 gcaccccgat cggggatcaa ttcggggcct tgctggggac gttcgcgttg cgcccgtccg
     9121 gctatctggc gctggcggcg caagcggcgc tgatcgcggc gatcacggcc tgggcgtcgc
     9181 ggcgaacgct gtttgccacg cttgacgaca tcgactgatg cggcttgcac gaataccgcg
     9241 ccggcccgac tcccattcgt ctgaaaccgt tgccaagcgt tttggagcga aagcctgccc
     9301 cggtttgcgt caagaagacg cgtcaaatcg ggaatccggc gccccgtttc gattctatcg
     9361 aaaccgaaaa gcctctagac tgaatgcgtg gagggtcgct ggcatcacat gagtctccag
     9421 cccgacgata aatcgccaaa cgcgccgagt tccgcgcggc ggggctggct gcgcgcgacg
     9481 atcgtggcgg tgtcggcgat cgcgttcgtc accgcggcga tcggctttgt cggttttctc
     9541 tcgaagttgc gcggtgccga gttgaagcct gcacgcaatg ccgacggcat cgtggtgctg
     9601 accggcggct cgtcgcgggt ttccgacgcc atggaactgc tcgccggcgg ctatggcaag
     9661 cggctgttga tctcgggcgt ccatccaacc aatgccgcaa gcgatatctc gcgctcgctg
     9721 ccggataacc agtcgttgct aagctgctgc gtcgatctcg accgctccgc cgtcaacacc
     9781 cgcagcaacg cggcgcaaac ccggcgctgg gcgcgcgacc gcggcttcaa gtcgctgatc
     9841 gtggtgacat cgaactacca catgccgcgg gcaatcgtcg aattgtcgca cgcgatgccc
     9901 gatgttgcgc tgattccgtt cgcggtggtc ggcgacaaat ggcgggatga gccgtggtgg
     9961 accaatggcg cgacattgcg gctgctgcta tcggagtacg tgaaatacgt cgccgccgag
    10021 gtgcgggtgc gcctggctga tgccggcctc gaattgacgc cggagcttgc ggatcagccg
    10081 agcggatctc tcgcgctgcc cagaccggcg acggctcaag ccaactgatc gggttttcga
    10141 tggtttcgat tttcttgcgt tcgctggtct acaacgtgct gttctatgcg ctgctggtgt
    10201 tctggattct gattgcaatc cccacctatc tgatgccgcc gcgggccttc atgaccgtcg
    10261 ccaaagcctg ggcgcgcagc agcgtctggc tcatgcgcgt gatctgcaac accaaagtgg
    10321 aatatcgcgg gctggagaaa attccgaaag ggccgctgat cgtcgcgtcc aagcatcagt
    10381 cgatgtggga aaccttcgcg ctgctgcagt tcttcgatgc gccgctgttc atctacaagc
    10441 gcgaactggg ctggatt
//
LOCUS       DATNOI010000500         2869 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1485984,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000500 DATNOI010000000
VERSION     DATNOI010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485984"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(395..1123)
                     /gene="istB"
                     /locus_tag="VN968_20205"
     CDS             complement(395..1123)
                     /gene="istB"
                     /locus_tag="VN968_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043766072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HXL31593.1"
                     /translation="MERTDVLELMATLKLYGMRAAYDEVMTTGIKRRHEPPRIVGDLL
                     SAEIAEKQARSIKYQLTIAKLPLAKDIDDFDFTGTPVNQALIRDLASGAFLAEQRNAV
                     LIGGTGTGKSHLAIAIARACIRGGARGRFYTVVDLVNRLESEARASRQGRFAEYLTRM
                     DFIILDELGYLPFAQTGGQLLFHLISRLYERTSVILTTNLDFGDWPSVFGDAKMTTAL
                     LDRLTHHCEIIETGNESWRFKNRA"
     gene            complement(1056..2622)
                     /gene="istA"
                     /locus_tag="VN968_20210"
                     /pseudo
     CDS             complement(1056..2622)
                     /gene="istA"
                     /locus_tag="VN968_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200010.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          504 a          939 c          913 g          513 t
ORIGIN      
        1 taatctaatc gaaacgcgtt ttcgaaggct ggtcaggcgg attcgccgca ttcccgctaa
       61 tcgactgtgt gctcaccatc gccactggcg cgtgttgcgg atgacgaagc ttccaggaac
      121 ttcggatcat cgagatcgcc attgacggag ttccgggtcg tggaacaaca ggactggcgg
      181 ctcgtgcggc atttcgagaa cacatccgtg tcaaacggcg cgcaatcggg accccggatc
      241 ggcatggaaa agggacccct cgctggcatc gggacaggct tgtcccggta gcgaacagga
      301 gggccccgcg cgcgtcgcgt agtgccctca cgagcgacgg agcggcgcgc gtgggaggtg
      361 cctgtttgcc caccggggca agccggggtg gcactcaggc gcggtttttg aagcgccagc
      421 tctcgttgcc ggtttcgatg atttcgcaat gatgggtcag gcgatcgagg agcgccgtcg
      481 tcatcttggc atcgccaaac acactgggcc agtcgccaaa gtccagattg gtggtgagga
      541 tgacggaagt gcgctcgtaa agccggctga tcagatgaaa cagcagctgg ccgccggttt
      601 gagcgaaggg caggtagccg agctcgtcga gaataatgaa gtccatgcga gtgagatatt
      661 cggcaaaccg cccctgccgg ctggcgcggg cttcgctctc cagccggttg acgaggtcga
      721 cgacagtgta gaagcggccc ctggcgccgc cgcggatgca agcccgcgca atagctatgg
      781 ccagatgcga cttgccggtg ccggttccgc cgatcagcac ggcgttgcgc tgttcggcga
      841 gaaaggcgcc gctcgccaga tcgcggatca gcgcctggtt gaccggtgtg ccggtgaagt
      901 cgaagtcgtc gatgtccttg gccagcggca gcttggcgat ggtcagctga tacttgatcg
      961 agcgcgcctg cttctcggcg atctcggcgc tgagcaaatc gccgacgatg cgcggcggct
     1021 cgtgccggcg cttgatgccg gtggtcatca cctcgtcata ggcggcccgc atgccgtaga
     1081 gcttgagggt ggccatcagc tcgagcacat cggtgcgttc catcacgaag ctctccgcaa
     1141 gccgtcgtag cgggcgcaat cggcgatcgg cgggtgtcgc agccgcagcg cgtcgggggt
     1201 caggatcgtc gccaccggcc cgggatcgcg gcgccgcgcc aggatgttga gaacaacgtc
     1261 ggccgaatgg accccgccgg cgagggcttc ggcgcaggcc gcctctactg cggcgaggcc
     1321 gtcggtcagc accgcggaga ggatcttgac catctggcgc tcgccgtcgt cggagccctt
     1381 cagcatgcgc tgcacgcgct ccaggctggc cggcagcagc caagccttga agggggctcc
     1441 gttgcgcagc gcgccgggct ttcgggcgag caccggcacg taatgccagg gatcgtagct
     1501 cgtctcgccg cggccgaagc ggcgctgatg ttcgccgacg atcgcgccgt cctggcggat
     1561 cacgacacga tcggcatagg cctggatctc gaccggccgg cccacggcgc gcgaggcgac
     1621 cgagtacttg ttgttgtcga accgcaccag gcaggtcttc gagaccgagg cttgcgtcgc
     1681 gtggaacccg tcgaacgggc cgcggattgg taccagctgg gggcgctccg cctcgaatgc
     1741 ctgccagatc gtgcagtcgg ccagctcggg atgcttgtgc gccttggcgt aagcgacgca
     1801 ccggtcgagc agccaagcgt tcagctcctc atagctcgct acccgcagcc gcggcgtgaa
     1861 gaaacgctcg cgcaccaatc caacctggtt ctcgacctgg cccttctccc agcccgaagc
     1921 cggcgtgcag gcgacgggtt caaccagata atgtccgcac atccgcagaa aacggcggtt
     1981 gaactggcgg tccttgccga cgaacacggc ctcgaccgcc gtcttcatgt tgtcgtagat
     2041 gccgcgcgtg caggtcccct tgaagaaagc aaaggcgcgg ttgtgcgcgt cgaacatcat
     2101 ctcctgggtc tcacgcggat aggcgcgcac gtacagcatg cggctatagc acaaccacac
     2161 atgcgcgacc ttgaccgtca ctgtcgtgcc gttgatcagc acgacctcgt ggctccagtc
     2221 gaactggtac gcctcgcccg gcgcaaagct caacggcaca taggcttccg ccatcgcggc
     2281 gccgcgctta gcacgccaca ccttcgcgta gcgtcgaacc gcatcgtagc tgccctcata
     2341 accgagggcg cgtagctcct cgaatatccg gatcagcgtc agccgctcgc gcgccggctt
     2401 ctcctcgttc gccagcagca gaccgtcgag ctggtcgcga caaggatcaa tccgcggcaa
     2461 cggctgcctg ccgcgctcat agcgaaactc ggttgcctct gaccggatca ccttgcgcac
     2521 gaccttccgc gacatccgaa actcgcggca aatcgccttg atcggcttct tctgaacgaa
     2581 aaacgcgcgc cgaatcttcg cgatcgtttc cactaccagc atccccaacc tggcttccat
     2641 gtccctcatg gaagccactg tggacccgcc gccccggggt cccgattgga tgccgatcac
     2701 cccgaaaacg gggtccttat tccatgccga atcacatctt ccgtgtccgt ttcatgtcct
     2761 gctcccgcgc cgtcggcgct ttctaggggc agcgcctcac cttagcctac ggtctcatct
     2821 tcgggggcca gctcacggcg tcgacgacgc ctgaagcagc tcttcatcg
//
LOCUS       DATNOI010000501         9931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1844044,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000501 DATNOI010000000
VERSION     DATNOI010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1844044"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="VN968_20215"
     CDS             <1..1495
                     /locus_tag="VN968_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HXL31594.1"
                     /translation="SRKPKLTISGLFVNTMAIVGLPDKAVSEARERVRSALIASGLAL
                     PARRITVNLAPADLPKEGSHYDLPIALGLMAAIGAIPPDALSGFTVLGELGLDGSIAP
                     VAGVLPAAIGANSRDESLICPAGCGAEAAWASPDIQIVAAHSLIQIANHFKGTQVLSR
                     PSPKVHEAEANLLDLRDIKGQESAKRALEIAAAGGHHLLMIGSPGAGKSMLAARLPSI
                     LPPLSPSELLEVSMIASVAGEIRDGALTARRPFRSPHHSASMAALTGGGTRAKPGEIS
                     LAHQGVLFLDELPEFDPRVLDSLRQPLENGEVAVSRANHRVTYPARFMLVAAMNPCRC
                     GHAYEPGYSCKRGRSDRCTGDYQARISGPLMDRIDLRIEVPAVTAADLILPPPTEGSA
                     EVAARIAAARDIQLARYAAAGLPHVRTNAEAPASVLEEIAKPDAHGLKLLRDAAETMR
                     LSARGYHRVLRVARTLADLDGADKIGRLHLAEALSYRALAEDVRSAA"
     gene            1613..2050
                     /locus_tag="VN968_20220"
     CDS             1613..2050
                     /locus_tag="VN968_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31595.1"
                     /translation="MLRFKILASAVPLIVAAVLARGEFSINSRPCIAIGGASVQITAS
                     PWQAQLQVSFTSDPSSAGAADFAVVDDVDSAEDGSCTANAATRFVAISANPLKSAPII
                     YLSPDGPADYRIFVHSKRFTASDAAALIVGAGGVPSRLAAASL"
     gene            2233..4503
                     /locus_tag="VN968_20225"
     CDS             2233..4503
                     /locus_tag="VN968_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL31596.1"
                     /translation="MGRGFRIKMRMRRFTRRHPWLTSTVRSFMIFSAAFGGAYGFIAG
                     SRSEDSGYDPHAFAIGASFLFAVACVALATLSMRLRWMRRKLRKIALHNEALADRNWE
                     LQEAEQRSRSLFESQDDLIVLRDARGCITFVNDAYCELAQLPRRALVGSDFTLDVLEQ
                     GDPALEQNGTRIHDQKIATAIGPRWIAWHEGLVRIDAGRPAEMQCVGRDVTDRTETER
                     GLAEARDLADAANRAKSRFLAMASHEIRTPLNGIIGMSGLLLDTPLTPEQMTYAKAVK
                     TSGDALLSLIEELLDYSKIEAGKIDLEHRPFALAALIEDITELLAPRAQARKLEIAAY
                     VDERLPMEVVGDAARLRQVLLNLAGNAIKFTSTGGVALIVEPGISPDEISFLVRDTGI
                     GIAPEAQQRIFREFEQADEHIARSYGGTGLGLSISERIVRRMGGRITLESEPGAGSTF
                     EVSVPLAASDDAAGEQRAFAAPDLTAQSIMLVAPQSIEASLIARRLQRWGGQTCLVSD
                     VAVAQALLPERSWDAVLLDQALGTADVELLGNAARPHATQRIVMLTPAARHELQLSSA
                     SSAASAFTGYLVKPLRAASLAARLTMTPEIPAPNLAEIAPAEASDLLGTPMTAPVQGL
                     SVLVAEDNEINALLMRSLLTRLGHHAVVCSNGETALESWRSARSSGAPYDLVLMDVQM
                     PQLDGLETTKRIRDLEAGHPGRQTPILALTANTLAEDRYACFESGMDGFLIKPLDREK
                     LADALAGLAASRHLAA"
     gene            4764..5717
                     /locus_tag="VN968_20230"
     CDS             4764..5717
                     /locus_tag="VN968_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL31597.1"
                     /translation="MIPSGDAGIQLFVRNKHPAGRETSPDKILLFVHGATYPAETAFD
                     LPIEGVSMMDLIAARGYDVYLVDVRGYGRSTRPAEMSQPPAANKPIVSTKVAAHDLGI
                     AVDYILKKRKVSKVNVMGWSWGTSIAGSYTSEHNDKVNRLVLYAPQWIRNEPPAPAAA
                     NAPPLGAYRLVSKDSAKARWLKDVPADKQADLIPPGVFDAWANATWATDPESSRQNPP
                     MLRAPNGVVEDSVNYWSAGKALYDPGKITVPTLLLHAEWDADLPSYLAQGYFAQLKNT
                     PYKRLIELSEGTHTVMMEKNRMQFFRELMGFLDEEKPLALK"
     gene            5821..6543
                     /locus_tag="VN968_20235"
     CDS             5821..6543
                     /locus_tag="VN968_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXL31598.1"
                     /translation="MKSPKSTDTIMLKRERPCDQAKWLKGKRATMKTLQIICAVLLFL
                     LGCSPASAEDSRELVSALKQGGHVIVFRHGATDDSQKDIYPLNFDDMKAQRQLSEKGR
                     DMARQIGTAIKKLGIPIGEIYTSRLNRAIETGKLLSDKEVKPVDALTDSGAGSASAMA
                     NPAGGNAKAGLAMRELVNATPKTGTNTLMVTHKTNIADAFGKEASDAQEGEAFVYRSS
                     GSAPAAFVARVKPAFWIEQAAN"
     gene            6709..7653
                     /locus_tag="VN968_20240"
     CDS             6709..7653
                     /locus_tag="VN968_20240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL31599.1"
                     /translation="MQNGEFAQRFIQMLRPENVGNAAQTAITWLKPPPPIGIKPLRLA
                     NFPASLGRLGALEVRLAQAKRDVKRAQKLRYRVFYRDGTAVADAATLLARRDKDAFDK
                     ICDHLMVIDHAAKPSIGGKPPVVGTYRLLRQDVAESHGGFYTEDEFDIAGLVERHAGL
                     RFLELGRSCVLPRYRNKRTVELLWHGIWSYVRQHRLDVMIGCASFEGTDPDRLKLPLS
                     FLHHYAQAPEDWRAKAHPSRRVEMNRMAKEAINPKAALHELPPLIKGYLRLGAYIGDG
                     AVIDHQFGTTDVLIVMPVSAINARYIEHFGVDASRHAA"
     gene            complement(7796..8752)
                     /gene="coaA"
                     /locus_tag="VN968_20245"
     CDS             complement(7796..8752)
                     /gene="coaA"
                     /locus_tag="VN968_20245"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HXL31600.1"
                     /translation="MDIRAPEQQYNPYRIFSREQWARLRDDTPMTLEPGEFERLRSLH
                     DRLDLQEVEDIYLPLSRLLSIYVDAMLRLYQAQRQFLGIRDRKVPYIIGVAGSVAVGK
                     STTARVLQALLARWSPRPKVDLVTTDGFLFPNAVLERQGLMQKKGFPESYDLPTLLAF
                     LSDIKAGRRPVRAPVYSHLTYDIIPNQWTEVDRPDILIVEGVNVLQTGRLPRDGKAVP
                     VVSDFFDFSVYIDADESVLREWYVRRFLALRDTAFHDPRSYFHRYAPLSDEEATATAL
                     AIWERTNLANLEENILPTRPRATLILKKGADHMVETVALRRL"
     gene            complement(8763..9089)
                     /locus_tag="VN968_20250"
     CDS             complement(8763..9089)
                     /locus_tag="VN968_20250"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HXL31601.1"
                     /translation="MPRFTIHDLAATIDVRADSGGETSYTRKLLDKGSEHCAKKFGEE
                     AVETVIAAVENDRGHLIAESADLLFHLLVLLKSRGVTLGDVEAELARRTTMSGLEEKA
                     ARKRDS"
     gene            complement(9169..9930)
                     /gene="hisF"
                     /locus_tag="VN968_20255"
     CDS             complement(9169..9930)
                     /gene="hisF"
                     /locus_tag="VN968_20255"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HXL31602.1"
                     /translation="MIPCLDVKDGRVVKGVNFVDLRDAGDPVEAAIAYDAAGADELCF
                     LDITATHENRGTMLDVVRRTAEACFMPLTVGGGVRTVDDIRVLLRSGADKVSINSAAV
                     SRREFVKEAAEKFGDQCIVVAIDAKRVKRGGGGERWEIFTHGGRNSTGIDAIEYAQEV
                     VSLGAGEILLTSMDRDGTRQGFDIPLTQAVADSIPVPVIASGGVGHLDHLVDGIREGH
                     ATAVLAASIFHFGEFTIREAKDHMVRAGLPMRLDP"
BASE COUNT         1854 a         3185 c         3106 g         1786 t
ORIGIN      
        1 gtctaggaaa cccaagctta ccatttcggg gttatttgta aacactatgg ccattgtcgg
       61 gctgcccgac aaggcggttt ccgaggcccg cgagcgggtc cgctcggcac tgatcgcctc
      121 cggcctcgcg ctgccggcgc ggcgaatcac cgtcaacctc gcccccgccg acctgccgaa
      181 ggagggcagc cactacgatc tgccgatcgc gctcggactg atggcggcga tcggcgcgat
      241 accgccggac gcgttgagcg gcttcacggt gctcggcgaa ctcggcctcg acggatcgat
      301 cgcgccggtg gcgggggtgt tgccggcggc gatcggcgcc aattcgcgcg acgaaagcct
      361 gatctgtccc gcggggtgcg gggcggaagc ggcgtgggca agcccggata tccagatcgt
      421 cgcggcccat tcgctgatcc agatcgccaa ccatttcaag ggcacccagg tgctgtcgcg
      481 cccgtcaccg aaggtccacg aggccgaagc caacctgctc gacctgcgcg acatcaaggg
      541 acaggaaagc gccaagcgcg ccctcgagat tgccgcggcc ggcggacatc atctgctgat
      601 gatcggttct cccggcgccg gcaaatcgat gctggcggcg cggctgccct cgatcctgcc
      661 gccgctgtcg ccgtccgaac tgctcgaagt ttcgatgatt gcctcggttg ccggcgaaat
      721 ccgcgacggc gcgctgacgg cgcggcggcc gttccgcagc ccccatcatt ccgccagcat
      781 ggctgcgctg accggcggcg gcacgcgcgc caagccgggc gaaatctcgc tcgcgcatca
      841 gggcgtgctg tttctcgacg aattgccgga gttcgatccc cgggtgctgg attcgctgcg
      901 ccagcccttg gagaacggcg aggtcgcggt gtcgcgcgcc aatcatcgcg tcacctatcc
      961 ggcacgcttc atgctggtcg cggcgatgaa cccttgccgc tgcggccatg cctatgaacc
     1021 cggctattcc tgcaagcgcg ggcggagcga ccgctgcacc ggcgactacc aggcacgcat
     1081 ctccggtccg ctgatggatc gcatcgactt gcggatcgag gtgccggcag tgaccgccgc
     1141 cgatctgatc ctgccgccgc cgaccgaagg atccgccgag gtcgccgcgc gcatcgccgc
     1201 ggcgcgcgac attcaattgg cgcgttacgc cgcggccgga ctgccgcatg tccgcaccaa
     1261 tgcggaggcg ccggcgtcgg tgctggaaga aatcgccaag cccgacgcgc atggcctgaa
     1321 actgctgcgc gacgccgccg agaccatgcg gctgtcggca cggggctatc atcgcgtgct
     1381 gcgcgtcgcc cgcacgctgg ccgatctcga tggcgccgac aagatcggcc ggctgcacct
     1441 ggccgaagcg ctgtcctacc gcgcgctcgc cgaagatgtg agaagtgcgg cgtgaagcat
     1501 cgaccgcttt gtcgtggcat cggcacgtga acgcgctggt aacgagtttt gtttacattc
     1561 cgccaaccat aagcccggcg ttttctccgg cttgtctgcg ggcgggcgta tcatgttgcg
     1621 tttcaagatt cttgcttcgg ctgtgccgtt gatcgttgcc gccgtgctcg cccgcggcga
     1681 gttctcgatc aactcgcgcc cctgcatcgc gatcggcggc gcatcggtcc agatcacggc
     1741 ctcaccctgg caagcacagc ttcaggtcag tttcaccagc gacccgtcga gcgccggcgc
     1801 ggcggatttc gccgtggtcg atgatgtcga cagcgcggag gacggctcct gcaccgccaa
     1861 cgcggcgacc cgatttgtgg cgatctccgc aaatccgctg aagtccgcgc cgatcatcta
     1921 tctctcgccg gacggccccg ccgattatcg gattttcgtg cattcgaagc gttttacggc
     1981 aagcgatgcg gcggctctta tcgtcggcgc cggtggcgta ccgagccgcc tcgccgccgc
     2041 gtcgctctga ggcgccttaa cgctttatca accatgttct catcgggcgg cgtgacgtcg
     2101 caccgactca agtacttttt caagatcggc gccgcatggt cttcggcagc acatggcgcg
     2161 cgtcgaggca gtctcaatgc cgatcgcgcc aggcgatcac gctgccagaa ggcaatctca
     2221 ggagagttgg ctatggggcg gggtttccgg atcaagatgc gcatgcgtcg cttcacgcga
     2281 cgtcatccct ggctcacttc caccgtccgc tcgttcatga tcttctccgc cgcctttggc
     2341 ggtgcgtatg gattcattgc cggcagccga tccgaagatt ccggctatga cccccatgcc
     2401 ttcgcgatcg gtgccagctt cctgttcgcg gtggcttgtg ttgcgcttgc gaccttgagc
     2461 atgcggctgc gctggatgcg caggaagctg cgcaagatcg ctctgcataa cgaagcgctg
     2521 gccgaccgca actgggaatt gcaggaagcc gagcagcgct cccgcagcct gttcgaatcg
     2581 caagacgacc tgatcgtcct gcgcgatgcc agaggttgta tcactttcgt caacgacgcc
     2641 tattgcgaac tggcgcagct gccgcgccga gcgctggtcg gcagcgattt cacgctcgat
     2701 gttcttgagc agggcgatcc ggcgctggaa cagaacggta cgcggattca cgaccagaaa
     2761 atcgcgaccg cgatcgggcc gcgatggatc gcatggcacg agggcctggt ccgtatcgat
     2821 gccggccggc cggcggagat gcaatgcgtc ggccgcgacg tcaccgaccg caccgagacc
     2881 gaacgcgggc ttgccgaggc ccgcgacctt gccgacgccg ccaaccgcgc caagtcgcgg
     2941 ttcctcgcaa tggcatctca tgagattcgc acgccgctca acggcatcat cggcatgagc
     3001 gggttgctgc tggatacgcc gctgacgccg gagcagatga cctatgccaa ggcggtgaaa
     3061 acctcaggcg acgccttgct gtcgctgatc gaggaactgc tggattattc caagatcgag
     3121 gccggcaaga tcgatctcga acatcgcccg ttcgcgctgg ccgcattgat cgaggatatc
     3181 accgaactgc tggcgccacg ggcgcaagcc agaaagctcg agatcgcagc ctatgtcgac
     3241 gagcggctgc cgatggaggt ggtcggcgac gccgcacggc tgcggcaggt gctgctcaac
     3301 ctcgccggca atgcgatcaa gttcacctcg accggcggcg tggcgctgat tgtcgagccg
     3361 gggatctcgc ccgatgaaat cagcttcctg gtgcgcgata ccggcatcgg catcgcgccc
     3421 gaggcccagc agcggatttt ccgggagttc gaacaggccg acgaacacat tgcccggagc
     3481 tatggcggca caggcctcgg cttgagcatc tccgagcgca tcgtcaggcg aatgggcggc
     3541 cgcatcacgc tggagagcga gccgggtgcc ggctcgacat tcgaggtgtc tgtcccgctc
     3601 gccgcctccg acgacgccgc cggcgaacaa cgggcatttg cggcgccgga tttgacggct
     3661 caatcgatca tgctggtcgc gcctcagagc atcgaggcat cgctgatcgc ccggcggctg
     3721 caacgctggg gcggtcagac ctgcctggtt tcggatgtcg cggtggcgca ggccctgttg
     3781 ccggagcggt cctgggacgc cgttctgctc gatcaggccc tgggcactgc cgacgtcgaa
     3841 ttgctgggta acgccgcgcg cccgcacgcg acgcagcgga tcgtgatgtt gacgccggcg
     3901 gcgcgccatg agcttcagct gtcctccgct tcctcggccg cctctgcttt caccggctat
     3961 ctggtcaaac cgttgcgcgc ggcttcgctt gcggcccgct tgacgatgac accggaaatt
     4021 ccagccccca acctcgccga aattgccccg gccgaggcgt ctgatctttt ggggacgccg
     4081 atgaccgcgc cggtgcaagg tctttccgtt ctggtggcgg aggacaatga gatcaacgcg
     4141 ctcttgatgc gttcgctgtt gaccaggctc ggacatcacg ccgtcgtctg cagcaacggc
     4201 gaaacggcgc ttgaatcctg gcgttcagcc agatccagtg gcgcgccata tgatctggtt
     4261 ctgatggacg tccagatgcc gcaactcgac ggcctcgaaa ccaccaagcg gatccgcgac
     4321 ctggaggccg ggcacccggg ccgccagacg ccgatcctcg cactcacggc gaacacgctg
     4381 gcggaggacc gctacgcctg ctttgagtcc ggcatggacg gtttcctgat caagccgctc
     4441 gatcgtgaaa agcttgccga cgcgcttgcc ggtttggccg cctcgaggca tctcgccgcc
     4501 tgaaggctgc acgacgcgcc gaccgctcgc gcgataccct tgacgctgcc ggcgattgga
     4561 ccgagcatgt gagagctacc cggtccgctc aagcggactg gaatagcagg ctgccgacgc
     4621 gcggcccaag aaagcaaaaa cgtcccggga ggacttcaat gaccaacagg tttttacagc
     4681 tatctctggc gtgtcttgtc gcaagcacct tcgcgagcgc aggcgcacag gcgaaggctc
     4741 caaagttgga aacaacggat accatgatcc cgtcaggcga tgcgggtatc cagttgtttg
     4801 tgcgcaacaa gcatccggcc ggccgcgaaa cttctccgga caagatcctg ttgttcgtgc
     4861 atggcgccac ctatccggcc gagaccgcgt tcgatctgcc gatcgagggc gtttcgatga
     4921 tggatctgat cgcggcgcgc ggctacgatg tctatctcgt cgatgtcagg ggttacggtc
     4981 gctcgacacg gcccgccgaa atgagccagc cgccggcggc gaacaaaccg atcgtgtcga
     5041 ccaaggtcgc ggcgcacgat ctcggcatcg ccgtcgatta catcctcaag aaacgcaagg
     5101 tttcaaaggt caacgtcatg ggctggtcgt ggggtacctc gatcgccgga tcgtacacca
     5161 gcgagcacaa tgacaaggtc aaccgtctcg tgctctacgc gccgcaatgg atccgcaacg
     5221 aaccgccggc gcctgcggcc gccaatgcgc cgccgcttgg cgcctatcga ctggtctcca
     5281 aggactcggc caaggcgcga tggctgaagg atgtccccgc cgacaagcag gcggatttga
     5341 ttccgcccgg cgtgttcgat gcctgggcga acgcaacctg ggcgaccgat cccgagtcat
     5401 cgaggcagaa cccaccaatg ctgcgtgcgc cgaacggcgt tgtcgaggac agcgtgaact
     5461 actggagcgc gggcaaggcc ctgtacgacc ccggcaagat taccgtgccg acccttctgc
     5521 ttcatgccga atgggatgca gaccttccga gctacctcgc gcagggctat ttcgcgcagt
     5581 tgaagaatac accctacaag cggctgatcg aactgagcga gggaacgcat acggtgatga
     5641 tggaaaagaa ccgcatgcaa tttttccgcg aactgatggg attcctcgac gaggaaaaac
     5701 cactggcgtt gaaataaacc gccaaattag acgtcttcgg atggaatgcc ggccggatgg
     5761 ccggcattct gcgtttgagc acatggttgc cggcaacggc tttatcctcc gatcggctga
     5821 ttgaaatctc cgaaaagcac cgacaccatc atgttgaaaa gagaaaggcc ttgcgatcaa
     5881 gcaaaatggc taaaagggaa aagagccacg atgaaaacgc ttcagatcat ctgcgcggtc
     5941 ctcctgtttt tgctcggttg ttcgccagcg tccgcggaag attcgcgaga gcttgtgtca
     6001 gcgttgaagc agggaggtca tgtcatcgta ttccgtcatg gcgccaccga cgacagccag
     6061 aaggatattt atccgcttaa ttttgatgac atgaaagccc aacggcagtt gagcgagaag
     6121 gggcgggaca tggctcgcca gatcgggact gcgatcaaga agctcggcat tccgatcggt
     6181 gagatctaca ccagccgcct caatcgggcg attgaaaccg gcaagttgct gtccgacaag
     6241 gaggtgaaac ccgtcgatgc attgacggac agcggcgcgg gaagcgcgtc tgcgatggcg
     6301 aaccccgccg gcggcaacgc aaaggcaggc ctcgccatgc gggagctggt aaatgccaca
     6361 cccaaaaccg gcacgaacac cttgatggtt acacacaaga ccaatatcgc ggatgcattc
     6421 ggaaaagagg cgagcgatgc gcaggaaggc gaagccttcg tctaccgatc cagcggatcg
     6481 gctcctgcgg catttgtcgc ccgcgtaaag ccggcattct ggatcgagca ggccgccaac
     6541 tgatccagcc ggctccagac gccagtcggc ggctgtcgcc ttgaagatct tgcgcaggct
     6601 gtcacacccg ggcaatgacg gtgtcacatg gctgttgaac gcccgtgatt tctgaacccg
     6661 aagaccgcgc cgatttcgaa tcggacagat gcggtcgaag ggatgtccat gcagaacggc
     6721 gaatttgcgc agcgcttcat tcagatgctg cggccggaga atgtcgggaa cgcggcgcaa
     6781 accgcgatca cctggctcaa gccgccgccg ccgatcggca tcaagccctt acgtttagcc
     6841 aattttcctg cctcgctggg gcggctaggc gcgctcgaag tgcggctggc gcaggcaaag
     6901 cgtgacgtga agcgcgccca aaaactgcgt tatcgcgtat tctacaggga tggcacggcg
     6961 gttgccgacg ccgcgacgct gctggcgcgc cgcgacaagg atgccttcga caagatctgc
     7021 gatcatctca tggtgatcga tcacgccgcc aaaccgtcga tcggcggaaa gccgccggtg
     7081 gtcggcacct atcgcctgct gcgccaggac gtggcggaaa gccacggcgg tttctacacc
     7141 gaggacgagt tcgatatcgc cggtctcgtc gagcgtcatg cgggcttgcg cttcctcgag
     7201 ctcggacggt cctgcgtgct gccgcgctat cgcaacaagc gcaccgtcga attgctgtgg
     7261 cacggaatct ggagctatgt ccgccagcac cggctcgacg tgatgatcgg ctgcgccagc
     7321 ttcgagggca ccgacccgga ccggctcaaa ttgccgctgt cgttcctgca tcactacgcg
     7381 caagccccgg aagactggcg cgccaaggcg catccctcgc gccgggtcga gatgaaccgg
     7441 atggccaagg aagcgatcaa tccgaaggcc gcccttcacg agctgccgcc cctgatcaag
     7501 ggatatctgc ggttaggggc ttatatcggc gatggtgcgg tgatcgatca tcagttcggc
     7561 accaccgacg tgctgatcgt gatgccggtg tcggcgatca acgcccgcta tatcgagcat
     7621 ttcggcgtcg acgcgtcgcg gcacgcggct taaggcgcga ttcatgcacc gtcattccgg
     7681 gcgtgagccc ttgcgagcga acccggaatc cagcgatgat caggcgcgag attccgggtt
     7741 cgcgctaacg cgcgccggaa tgacaaaaat tgagtccatc caaaacatat cgacgctaca
     7801 gccgccgcag cgccacggtt tcgaccatgt ggtccgcgcc ctttttcagg atcagcgtcg
     7861 cgcgcggacg ggtcggcaag atattctctt cgagattggc gaggttggtc cgctcccaga
     7921 tcgccaatgc ggttgcggtg gcttcctcgt ccgacagcgg cgcgtagcga tgaaaatacg
     7981 atcgcggatc gtgaaacgcg gtgtcccgaa gcgccaggaa gcgtcggacg taccactcgc
     8041 gcagcacgga ttcgtcggca tcgatataga ccgagaaatc gaagaaatcc gaaaccacgg
     8101 gcacggcctt gccgtcgcgt ggcagccggc cggtctgcag gacgttgacg ccctcgacaa
     8161 tcaggatatc cggccggtcg acctcggtcc attggttcgg aatgatgtcg taggtcaaat
     8221 gcgaatagac cggggcacgc accggccgtc ttccggcctt gatatcgctc aagaacgcca
     8281 gcagcgtcgg cagatcatag ctctccggga agcctttctt ttgcatcagg ccttgccgct
     8341 cgagcaccgc gttgggaaac aggaaaccgt cggtcgtcac caggtcgacc ttgggccgcg
     8401 gcgaccagcg cgccaaaagc gcctgcagca cgcgcgcggt ggtcgatttg ccaactgcga
     8461 cggagcccgc gacgccgatg atgtagggca ccttgcgatc ccggatgccg aggaattggc
     8521 gctgcgcctg atagaggcgg agcatggcat cgacatagat tgacaacaga cgcgacagcg
     8581 gcagatagat gtcttcgact tcttgcagat cgaggcggtc gtgcagcgaa cgcaatcgct
     8641 cgaactcgcc tggctccagc gtcatcggcg tatcgtcgcg caaccgcgcc cactgttcgc
     8701 gcgagaatat ccggtaggga ttgtactgtt gctcaggcgc ccgaatatcc atgaagctac
     8761 ctttatgagt cccgctttcg cgcggccttc tcttccagtc cggacatggt cgtgcgccgc
     8821 gccagttccg cctcgacatc gccaagcgtc acgccgcgcg atttcagcag caccagcagg
     8881 tgaaacagca ggtccgcgct ttcggcaatg agatggccgc gatcattctc gaccgcggcg
     8941 atcacggtct ccaccgcttc ctcgccaaac ttcttggcgc aatgctccga acccttgtcg
     9001 agcaatttgc gggtatacga agtctcgccg cccgagtccg cgcggacatc gatggtggcg
     9061 gccaggtcat ggatcgtgaa acgcggcatc aactcaaccc ggcacgtgcg cccgcgggtg
     9121 ggacgccgct catcccatgg aacttaacac ttctgtcaca aagagtcgtc agggatcgag
     9181 ccgcatcggc agcccggccc gtaccatgtg atccttggct tcacgtatgg taaattcccc
     9241 gaagtggaaa atcgacgccg ccagcaccgc ggtcgcatgc ccctcgcgga tgccgtccac
     9301 caggtgatcg agatgcccca ccccgccgga ggcgatcacc ggcaccggga tgctatccgc
     9361 cacggcctgg gtcagcggaa tgtcaaaacc ctgccgggtt ccgtcccggt ccatggacgt
     9421 cagcaggatt tcaccggccc cgagcgagac cacctcctgg gcatattcga tcgcgtcgat
     9481 gcccgtggaa ttacgaccgc catgggtaaa gatttcccag cgctcgccgc cgccgccacg
     9541 cttgacgcgc ttggcgtcga tcgcgaccac gatgcactgg tcgccgaatt tttccgcggc
     9601 ctccttgacg aattcgcggc ggctgacagc ggcgctgttg atcgacacct tgtcggcgcc
     9661 ggatcgcagc agcacgcgaa tatcgtcgac ggtgcgcaca ccgccgccga ccgtcagcgg
     9721 catgaagcag gcttccgcgg tgcgccggac cacgtccagc atggtgccgc gattttcatg
     9781 ggtggccgtg atgtcgagaa agcacagctc gtcggcgccg gcggcgtcat aggcgatggc
     9841 ggcttccacc ggatcgccgg cgtcgcgcag atcgacaaag ttgacgccct tgacgacccg
     9901 gccgtccttg acgtcgagac aggggatcac g
//
LOCUS       DATNOI010000502         6279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2033941,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000502 DATNOI010000000
VERSION     DATNOI010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033941"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VN968_20260"
     CDS             complement(<1..940)
                     /locus_tag="VN968_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HXL31603.1"
                     /translation="MVRLISAAIVIGIFLILLGLASDFLVDWLWFSSIGYLQVFWTTI
                     GAKAIVFFAIWSATAVILWLNGWSAARFARRPPTPSVTGFAWNPAGNVPPPDLLTFMR
                     DRLPWPRVIAGSACLIALLVAAIDVGNWNVFLQFLYRVPFGADDPLYNKDISFYLFVL
                     PAYVIIKNWMLLTLVLSALFAGMIYRVFGDIEYDIHHRSMSPTAIAHGSALLGLLFVV
                     EAGSYGLDRYLLLYGDNGVVVGASYTDVHVKLPVLWLLIGLSIVAALASWANVWMRTY
                     RLPVAAVLLVAIGTLVLSGVTPALFRQLYVKPSELQL"
     gene            complement(1135..1635)
                     /locus_tag="VN968_20265"
     CDS             complement(1135..1635)
                     /locus_tag="VN968_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXL31604.1"
                     /translation="MKPEQIDHIDTTILSELATNARASQVDLAERVGLSSTAIARRQK
                     ALEEQGFIRGYQAVLDLTRFGLSTTVLVRVTLESQNEDALKSFEAAVIECPSVVRCFL
                     MSGSDDYIVIVMARDIEDFERIHRTELSRLPRVARIQSSFAMREVVNRAVPPVIFGGP
                     RRRGRT"
     gene            1774..2889
                     /gene="ald"
                     /locus_tag="VN968_20270"
     CDS             1774..2889
                     /gene="ald"
                     /locus_tag="VN968_20270"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HXL31605.1"
                     /translation="MRVGVPKEIKVHEYRVGLTPGAAREYVAAGHSVIVETNAGAGIG
                     ASDEAYRKAGAAIADTAAEIFASADLIVKVKEPQPREWTQLREGQIIFTYLHLAADPA
                     QAKGLMASGCTAIAYETVTDAKGGLPLLAPMSEVAGRLSIEAAGSALKRSAGGRGILL
                     GGVPGVAPARVVIIGGGVVGTHAARMAVGLGAEVTILDRSIPRLRELDELFTGRIRTR
                     FPTTEAIEQEVFAADVVIGAVLVPGASAPKLVTRKMLKSMQPGAVIVDVAIDQGGCFE
                     TSHPTTHADPTYEVDGIIHYCVANMPGAVPLTSSQALNNATLPFGLALANQGFAAVTQ
                     DPHLRAGLNVHRGRITNKAVAESLGLRYPPIEDPIAA"
     gene            complement(2938..3987)
                     /locus_tag="VN968_20275"
     CDS             complement(2938..3987)
                     /locus_tag="VN968_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HXL31606.1"
                     /translation="MSVNNKRVFYVKYLAHEIYADILRARPDVRLDRLENESPDEVSA
                     PILSAAHAYQIGAARDELAKHFHVDQDLLRRAPNLLIVSSNGAGYDPVDVEACTEAGV
                     LVVNQSGGNAHSVAEHALGMLLTLSKRILEADRALRREPNVNRNALVGTEAHGKTIGI
                     VGLGNVGRRIAALCKGLLGMKVLAYDPYLTASEMASRGGEKVELDELLSRVDYVSVSC
                     PLTSESRGMIGAREFALMQPHAYFITTARGFIHDEAALLEALRSKAIAGAGLDVWAKE
                     PPPPDHPLLQFDNVLASPHTAGVTREARANMGRIAAEQVLDALDGMRPPRLINPEVWP
                     RYIRRFERAFGFTPE"
     gene            complement(4002..4886)
                     /locus_tag="VN968_20280"
     CDS             complement(4002..4886)
                     /locus_tag="VN968_20280"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL31607.1"
                     /translation="MIPACLPPREVSRPKVPPPPNACDTHAHVFGPADRFPYTGDRSY
                     TPPDAPLEKYLDMLDTIGFARGALVQGSAHGRDNSAMLDALRQRPDRLRGVAVADADV
                     SPADLREWNRLGIRGLRFNHFFRDGQLHYRGGVPLSAARTLAPLMAELGWHLQLWIDA
                     KDLPDTIPVLKSLGRPVVIDHMGRTDARAGIGTEGFQALLRAVGDGWCWAKLSGAHRL
                     SRAAPDYADARPFHEALVRANPERLVWGGDWPHPRVEGEMPDAGHLFELFQLWTLDVA
                     IQHRILVTNPATLYGFPN"
     gene            complement(4903..5868)
                     /locus_tag="VN968_20285"
     CDS             complement(4903..5868)
                     /locus_tag="VN968_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL31608.1"
                     /translation="MAGWVRVAAAVAMFTLAGTASAQSPFPSKPVHIFVPYAAGGGVD
                     ILARTLGDVVSRHWGQTVVVENRPGAGGVVASQALVTSPPDGYTLIVVASGHATNPFL
                     YPKIPYDTFNDFAPISLLASSPNILLVRADSPFKTLADMIAQARSKPGSLSFAHAGTG
                     TSTHLAGELLKNLARIDLNAIPYKGGAPAINDLLGGQIPMSFNNGPESVGQIEAGTVR
                     ALAVTTASRASFLPNVPSMAETVPGYDTEVWWGLLGPAGMPRDLVEKISHDFVAALNT
                     EAVKERLTKLGASPIGSSPQQFDAKIRADYRKWGPIIEAAGIKPE"
     gene            6064..>6279
                     /locus_tag="VN968_20290"
     CDS             6064..>6279
                     /locus_tag="VN968_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31609.1"
                     /translation="MAAADGKTSPKTLDQNPSPETASSGKAWHDIVLQTLKRNDIRLV
                     PYVPDRVLTRLIQDLHADPFFTTFPTAR"
BASE COUNT         1243 a         1975 c         1976 g         1085 t
ORIGIN      
        1 caagctgcag ctcgctcggt ttgacgtaca gctgccggaa caacgcggga gttacgccgg
       61 acagcaccaa agtgccgatg gcgacgagca ggaccgctgc gacgggaagc cgataggtgc
      121 gcatccagac gtttgcccag gaagcgagcg ctgcaacgat cgacaggccg atcagcaacc
      181 acagcacggg caacttgaca tggacgtcag tgtagctcgc gccgacgacc acgccattgt
      241 cgccgtacag cagcagatat cggtcgaggc cgtaggaccc ggcctccacg acgaagagca
      301 gaccgagcaa cgccgagccg tgagcaatgg ctgtcggcga catcgatcga tgatgaatgt
      361 cgtattcgat atcgccgaac acccggtaga tcattccggc gaaaagcgcg ctcagaacga
      421 gggtgagcag catccagttc ttgatgatga cataggcagg cagtacgaag agatagaaac
      481 tgatgtcctt gttgtagagc gggtcgtccg caccaaaggg cacgcgatag agaaactgca
      541 aaaagacgtt ccaattgccg acgtctatcg cggcaaccag cagagcaatc aagcatgcgg
      601 agcctgcgat aacccggggc cacggcagcc gatcgcgcat gaacgtcaac agatcgggcg
      661 gcggcacatt gcccgcaggg ttccacgcaa aaccggtgac ggatggggtc ggcggccgcc
      721 gggcgaaacg tgccgcggac cacccgttga gccagaggat gacggctgtc gccgaccaga
      781 tggcaaaaaa gacgatggct ttagcgccga ttgttgtcca gaaaacctgc agatagccga
      841 tcgaggaaaa ccatagccaa tcgaccagga agtcgctcgc gagcccgagc agtatcagga
      901 aaatcccgat gacgatggcc gcactgatga gcctgaccac ggcgcttcgc cttggtacct
      961 tcggttcggg tccggtgatc ccgatcgtca taatcaaatc atagcaaaaa cgagagacgg
     1021 ttgccgcttt attcgcaatc gggaggacgc cttggggcaa aagtgacttt cgaaaagcag
     1081 gagcatcggc ctgttcgatg agcctgatcg atgccgtcgc gatgccgggc gcggttaggt
     1141 ccgtccgcgc cggcgcggtc cgccaaagat caccggcggg accgcccggt tcacgacctc
     1201 gcgcatcgcg aagctcgact gaatccgcgc cacccgtggc aatcgcgaca gctcggttcg
     1261 gtgaatgcgt tcgaagtcct cgatgtcgcg cgccatcacg atgacgatgt agtcgtcgct
     1321 gccggacatc aggaagcagc gcaccaccga tggacactcg atgaccgcgg cttcgaacga
     1381 tttcagcgca tcctcgtttt ggctttccag ggtgaccctg accagcacgg tggtggagag
     1441 accgaagcgc gtcaaatcca gaacggcctg atagccgcgg atgaagcctt gctcttccaa
     1501 tgctttctgc cgccgcgcga tcgcggtgct cgaaagcccg acccgctcgg caagatcgac
     1561 ctggctggcc cgggcgttgg tcgcgagctc cgacagaatc gttgtgtcga tatggtcgat
     1621 ctgctcgggt ttcatttgtt tggattcctg cgcaaagatg gaaatctagg ctggttcctg
     1681 acgaaaaatc gcaataatcc accgaaattg caagcactta aaccagaatc cgatctacat
     1741 attgggagag ggttcgaaac ggagtgccca gccatgcgcg tcggagtgcc caaagagatc
     1801 aaagtgcacg agtatcgcgt cggcctcacg ccgggcgcgg ctcgtgagta tgtagccgca
     1861 ggccacagcg tcatcgtcga gaccaatgcg ggtgcaggta tcggcgcgag cgatgaggcc
     1921 taccgcaagg cgggagcggc gatcgccgac accgccgccg agatttttgc gagcgccgat
     1981 ctgatcgtga aggtgaagga gccgcagcct cgcgaatgga cgcagctgcg cgagggccag
     2041 atcatcttca cctatctgca tctcgccgcc gacccggcgc aggccaaagg cctgatggcc
     2101 tcgggctgca cggcgatcgc ctatgaaacg gtcaccgatg cgaagggagg cctgccgctg
     2161 ctcgctccca tgagcgaggt ggcgggaaga ctttcgatcg aagcggcagg ctcggcgctc
     2221 aagcgcagcg caggcggccg cggcattctg ctcggcggcg tgccgggtgt cgcgcccgcc
     2281 cgcgtggtga tcatcggcgg tggcgtcgtc ggcacgcacg cagcgcggat ggccgtcggc
     2341 ctcggcgcgg aggtcacgat cctcgaccgg tcgatcccga ggctgcgcga actcgacgaa
     2401 ttgttcacgg gtcgaatccg gacacgcttc ccgaccaccg aggcaatcga gcaggaggtt
     2461 ttcgccgccg acgtcgtgat cggcgccgtt ctggtgcccg gcgcgagcgc accgaagctg
     2521 gtcacgcgaa aaatgctgaa gtcgatgcag ccgggtgcgg tgatcgtgga cgttgctatc
     2581 gatcagggcg gatgcttcga gacatcgcat ccgaccacgc acgccgatcc gacctatgag
     2641 gtcgacggca tcatccatta ctgcgttgcc aatatgcccg gtgcggttcc gctgacctca
     2701 agccaggcgc tgaacaacgc cacactgccg ttcggattgg ctctggcgaa ccagggtttt
     2761 gcggcagtca cgcaggatcc gcatctgcgg gcaggcctga acgttcaccg cggccgcatc
     2821 accaacaagg cggtggcgga gagccttggc ctgcggtacc cgccgatcga ggatccgatt
     2881 gccgcgtgag cggcgcctgg ctcccattgt cggggtaaac aatttatcgc cggaccccta
     2941 ttccggcgtg aacccgaatg cgcgctcgaa gcgcctgatg tagcgcggcc acacttccgg
     3001 gttgatgagg cgcggcgggc gcatgccgtc gagcgcatcg aggacttgct cggcggcaat
     3061 cctgcccatg ttcgcccgcg cttccctggt cactcctgcg gtatgcgggc tcgccagcac
     3121 attatcgaac tgcaacagcg gatggtcggg cggcggcggc tccttggccc agacgtcgag
     3181 acccgcgccc gcaatggcct tgctgcgcag cgcttcgagc agcgcggctt catcgtggat
     3241 aaagccccgc gccgtggtga tgaaataggc gtgcggctgc atcagcgcga attcgcgcgc
     3301 cccgatcatg ccgcggcttt cgctggtcaa cggacaactg acggagacgt aatcgacgcg
     3361 gcttaacagt tcatccagct cgaccttctc gccgccgcgc gaggccatct cgctcgccgt
     3421 gagataggga tcataggcca gcaccttcat accgagcagg cccttgcaga gcgcggcgat
     3481 gcggcgcccg acgttaccca gtccgacgat gccgatggtc ttgccgtgcg cctcggtgcc
     3541 gaccagggcg ttgcggttca cattcggctc gcgccgcagc gcgcggtcgg cctccaggat
     3601 ccgtttcgac aaggtgagca gcatgcccag cgcgtgctcg gccaccgaat gggcattgcc
     3661 gccggactgg ttgacgacca gcacgccggc ttcggtgcag gcctcgacat cgaccggatc
     3721 atagcccgcg ccgttgctcg agacgatcaa gaggttcggc gcgcgccgca gcaagtcctg
     3781 atcgacgtgg aaatgctttg ccaattcatc gcgcgccgcg ccgatctgat aggcatgcgc
     3841 cgcggataga atcggcgccg aaacctcgtc gggactttcg ttctcgagcc ggtcgagccg
     3901 cacatcgggg cgcgcccgca ggatgtcggc atagatctcg tgcgccagat atttgacgta
     3961 gaacacgcgc ttgttattga cggacattgt tgatgcgact ttcagttcgg gaagccatag
     4021 agcgttgcgg gattggtcac gagaatgcgg tgctggattg ccacgtccag agtccacagt
     4081 tgaaacaatt cgaacaggtg gccggcatcg ggcatctcgc cctcgacgcg cggatgcggc
     4141 cagtcgccac cccataccag ccgctccgga ttagcccgca ccagcgcttc atgaaatggc
     4201 cgcgcgtcgg cataatcggg cgcggcgcgg ctgaggcggt gggcaccgga tagcttggcc
     4261 cagcaccacc cgtcgccaac cgcccgcaac agcgcctgga aaccttccgt tccaatgccc
     4321 gcgcgggcgt cggtgcggcc catgtgatcg atcaccaccg gccggccgag cgatttcagc
     4381 accggtatcg tatccggcaa atctttcgca tcgatccaga gttgaagatg ccatccaagt
     4441 tcggccatca acggcgccag cgtccgcgcc gccgatagcg gcacgccgcc gcgatagtgc
     4501 aactgcccgt cgcggaaaaa atgattgaag cgcagtcccc tgataccgag ccggttccac
     4561 tcgcgcagat cggctggcga gacatcggcg tcggccaccg ccacaccgcg caggcgatcg
     4621 gggcgttgcc tgagcgcgtc cagcatcgcg gaattgtcgc ggccgtgcgc gctgccttgc
     4681 accagcgcgc cgcgcgcaaa tccgatggtg tcgagcatgt cgagatattt ttcgagcggc
     4741 gcatccggcg gcgtgtagct gcgatcccca gtgtaaggaa accggtctgc cggcccgaat
     4801 acatgcgcat gtgtatcgca ggcgttcggc ggcggcggca ctttcggccg gcttacctcg
     4861 cgcggcggga gacaggcagg aatcatccgc gtatccgctg catcactcgg gctttatgcc
     4921 ggcggcctcg atgatcgggc cccatttcct gtaatcggca cgaatcttgg catcgaattg
     4981 ctgtggcgag ctgccgatcg gcgacgcgcc cagcttggtc agccgctcct tgaccgcctc
     5041 ggtgttcaag gctgcgacga aatcatgcga gattttttcc accaaatcgc ggggcatgcc
     5101 ggccgggccg agcaaacccc accacacctc ggtgtcgtag cccggcacgg tctcggccat
     5161 gctgggtaca ttgggcagga atgacgcccg cgaagcggtc gtgacagcga gcgcccgcac
     5221 ggtgccggcc tcgatttgtc cgaccgattc cggtccgttg ttgaacgaca tcggaatttg
     5281 cccgccgagg agatcgttga tggcgggcgc gccgcctttg tagggaatcg cgttgagatc
     5341 gatcctggcg agatttttca gaagttctcc ggcgagatgc gtcgatgttc ccgtccccgc
     5401 atgcgcgaag gaaaggcttc ccggttttga gcgcgcctgc gcgatcatat cggcaagcgt
     5461 cttgaacgga gaatcggcgc gcaccagcag gatattggga gacgaggcca gcagcgaaat
     5521 cggcgcaaaa tcattgaagg tgtcgtaggg aatcttgggg tacagaaacg gattggtggc
     5581 gtgaccgctt gcgacaacga tgagggtgta gccatccggc ggcgacgtca ccagtgcctg
     5641 cgacgcaacc accccgccag cccccggccg attttcaacc acgacggtct ggccccagtg
     5701 tcgcgagacc acatcgccga gcgtgcgggc aagaatgtcc actcccccac cggccgcgta
     5761 ggggacgaaa atatgcacgg gcttggacgg aaacggtgac tgcgcggacg ctgtcccggc
     5821 caaggtaaac atcgccaccg ctgcggcgac ccggacccag ccggccatgg catgccctcc
     5881 caatgtcttt gttgcttcct ttatcgcatg ccaacacgcc aacggcaccg ccgtggcgtg
     5941 cagatcaagg atacgaccgg gacatgttga caatccccga gatcgcgtca agttttgcgc
     6001 ctcggcagaa tggccgcgat catgtcgcga acgaaaagtc atcgcgcagc aggggagatt
     6061 tcgatggcgg ctgccgatgg aaaaacctcg cccaagaccc tggatcagaa cccctctccc
     6121 gagaccgcgt cgtccggcaa ggcctggcac gacatcgtgc tgcaaacgct caagcgcaac
     6181 gacatccgcc ttgtgcccta cgttcccgat cgcgtgctga ccaggctgat ccaggatctt
     6241 cacgccgatc cgttcttcac cacctttccc accgcacgc
//
LOCUS       DATNOI010000503         2435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_324133,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000503 DATNOI010000000
VERSION     DATNOI010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324133"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..1308
                     /locus_tag="VN968_20295"
     CDS             7..1308
                     /locus_tag="VN968_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL31610.1"
                     /translation="MRLPFFYGWIIVVVTFVTMAIGVNARTAFSLFFPPIIDEFGWER
                     GVTAGAFSFGFLISGAVSPLIGRLMDRAGPRAVMELGVALMAGGLLLAPLTTQPWHLY
                     LTIGVMVGAGSVCLGYSGQSLFLPNWFIRRRGLAMGLAFAGVGIGSVTLLPWVQLMIE
                     QTGWRTACTAMGILILAVLAPINLLLRKRPEDIGLEPDGDAAPSASSAKPISNIVDPA
                     WAGTDWTLKRAIGTARFWWISLGYFCGLYIWYAVQVHQTKYLLDIGFSSSVAVWALGV
                     VSLLGIPGQILLGHLSDRMGREWIWAASCLGFAICFAALIALKYVPTLLLVYIMVLAQ
                     GALGYGLTSIMGAVVLEIFQGRHYGSIFGTIMLAALAGGAAGPWVTGFLHDLSGSYTI
                     AFAIGIGVSGLSAAAIWLASPGKIRAVAGQLHRIKAKPGAR"
     gene            1402..>2435
                     /locus_tag="VN968_20300"
     CDS             1402..>2435
                     /locus_tag="VN968_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXL31611.1"
                     /translation="MNEHVQAATTAPLFNPLAPEFIRDPYPFYERLRTTDPMHLTPFG
                     AFVASRHAEASHVLRDKRFGKDFVDRTVRRYGPQIMEQPVFRSMSHWMLQQDPPDHTR
                     LRGLVAMAFTARRVEDMRPRIQQVVDETLDAIVGQGHMDLIEDFAFRLPVTIICDMLG
                     IPEDHREQFYKSSRDGGRLLDPVPLSPAEIEQANAGNAMAQMYFQQLFELRRRNPGDD
                     LTTQLVQAEENGSKLSNEELTANIILLFGAGHATTANLIGNGLLALHRNPDQLALLKA
                     NPSVITNAVEEFLRYDSSVQMTGRVALEDIDDLGGKKIPKGESVLCLLGSANRDPAAY
                     PDRPDRLDITR"
BASE COUNT          407 a          767 c          757 g          504 t
ORIGIN      
        1 aacctgatgc gccttccctt cttttacggc tggatcatcg tcgtcgtgac gttcgtcacc
       61 atggcgatcg gcgtcaacgc ccgcactgcc ttttcgctgt tctttccgcc gatcatcgat
      121 gagttcggat gggagcgcgg cgtcacggcc ggcgccttct cattcggctt tctgatatcc
      181 ggcgctgtca gcccgctgat cggccgcttg atggaccgcg ccggcccgcg cgccgtgatg
      241 gaactcggcg ttgcgctgat ggcgggaggg ctgctgctgg cgccgctgac cacgcagcca
      301 tggcacctgt atctgacgat cggcgtcatg gtcggggcgg gaagcgtctg tctcggctac
      361 tccggtcaat cgttgttcct gcccaactgg tttattcggc ggcgcggcct ggccatgggc
      421 cttgcattcg cgggcgttgg aattggctcc gttacgctat tgccctgggt gcagctcatg
      481 atcgagcaga ccggttggcg aaccgcctgc acggcgatgg gcattctgat tttggccgtg
      541 ctggcgccga tcaatctgtt gctgcgaaag cgccccgaag acatcggact tgagccggac
      601 ggcgacgccg cgccttctgc gtcctccgcc aagccgatct cgaacatcgt cgatcccgcc
      661 tgggccggca ccgactggac attgaagcgc gcaattggca ccgcgcggtt ctggtggata
      721 tcgctcggct atttctgcgg attgtacatc tggtacgcgg tccaggttca ccaaaccaaa
      781 tatctgctcg acatcggctt cagttcttct gtcgcggtct gggcactggg tgtggtcagc
      841 ctgctcggta ttcccggcca aattttgctc ggacacctct ccgaccggat ggggcgtgaa
      901 tggatatggg ccgcgagctg cctgggcttt gcgatctgct tcgcggccct gatcgcgctg
      961 aaatacgttc cgacattgct cttggtctac atcatggttc tggcgcaggg cgcgctcggc
     1021 tatggcctga cctccatcat gggcgccgtg gttctggaga tattccaagg ccgacattac
     1081 ggcagcattt tcgggaccat catgctggca gcgctcgcgg gaggcgccgc gggcccttgg
     1141 gttaccggct ttctgcatga cctttcaggg agttatacga tcgcctttgc gatcggtatt
     1201 ggcgtgagcg gattgtccgc cgcggcaatc tggctggcgt cacccggcaa gattcgggcg
     1261 gtcgcgggcc agttgcaccg cataaaagcc aagcctggcg cgcgctagca agggtggtta
     1321 catacggctt gccgacatcg gcagcttgtg ggctaatcgc cttgcaaaag cagaatctga
     1381 ccgcatcgag ggcgccgatt catgaacgag catgttcagg ccgcgacgac cgcgccgctc
     1441 ttcaacccgc tggcgccgga attcattcgc gatccctatc cattctacga acggctgcgc
     1501 accaccgacc cgatgcacct cacgccgttt ggcgccttcg tcgccagccg gcacgccgag
     1561 gccagtcacg tgctgcgcga caagcgtttc ggcaaggact tcgtcgaccg caccgtgcgg
     1621 cgatacgggc cgcagatcat ggaacagccg gtcttccgca gcatgagcca ctggatgctg
     1681 cagcaggatc cgcccgacca cacccgcttg cgcgggctgg tggcaatggc gttcaccgcg
     1741 cgccgggtcg aggacatgcg tccgcgcatc cagcaagtcg tcgatgaaac gctcgatgcg
     1801 atcgtcggtc agggccatat ggacctgatc gaggactttg cgttccggct gccggtgacg
     1861 atcatctgcg acatgctcgg aattcccgag gaccaccgtg agcagtttta caagagttcg
     1921 cgcgatggcg ggcgcctgct cgatccggtg ccgctgtcgc cggccgagat cgagcaggcc
     1981 aatgccggta acgcgatggc ccagatgtat ttccagcagc tgttcgagct gcgtcggcgc
     2041 aatcccggag acgatctgac gacgcaattg gtgcaggccg aggaaaatgg cagcaagctc
     2101 tccaacgagg agttaacggc caatatcatc ctgttgttcg gcgcgggcca cgcgaccacc
     2161 gccaatctga tcggcaatgg gctgttggcg ctgcaccgca atcccgatca gcttgcgctg
     2221 ctcaaggcca atccttccgt catcacgaac gctgtcgagg aattcctgcg ctacgattcg
     2281 tccgtgcaga tgaccggccg ggtggcgctg gaagacatcg acgacctcgg cggcaagaaa
     2341 atcccgaaag gcgagagcgt gctctgcctg ttgggttccg ccaaccgcga cccggcggcc
     2401 tatcccgacc ggccggatcg tctcgatatc acccg
//
LOCUS       DATNOI010000504         2627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_260128,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000504 DATNOI010000000
VERSION     DATNOI010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260128"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..951)
                     /locus_tag="VN968_20305"
     CDS             complement(250..951)
                     /locus_tag="VN968_20305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXL31612.1"
                     /translation="MSATSFVIPALPQPSIPVAGDTKSFPVRRIWCVGRNYLEHIREM
                     GNDERAPPFFFAKHADMIEPDGATIPYPPLTKDLHHEVELIVALKSGGLDIPSDKALD
                     HVYGYGVGIDLTRRDLQLAARKKERPWEVGKSFDHSAPCGPLQPASKIGHPANGKIWL
                     SVNGTERQKGDLTELIWSVPEIIAKLSEQVTLGAGDIIMTGTPAGVAALSPGDRIECG
                     VDGIGTLKVTIGQPE"
     gene            complement(1020..>2627)
                     /locus_tag="VN968_20310"
     CDS             complement(1020..>2627)
                     /locus_tag="VN968_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31613.1"
                     /translation="VRGIAILSGVTILIFMLKGAATYGHSVILSQISNAILANNQRRL
                     FAKLMNESVAFFSERHSSEFLARLTSGANSVTQVLNLLINAVGRDVLSLIALVAVMLM
                     QDPYMALLGFLVAPPAMLVLRKLVKRIKGLAHNQFTGTADILETMQESLQGIRTVKAF
                     TLEQTMRDRIDASIAAVERNANKMARVSNRSSPLMETLGGFAIAGGLMYGGYSVVAMG
                     ATPGQFFSFLTAFLLAYEPAKRLARLNIELNSNLIGARKLLEIVDNPASEPGDDDRPA
                     LKLTDARVELRDVSFAYRLNEPVLNRMSFVAEPGKVTALVGPSGGGKSTVLALLLRFY
                     EATGGDILIDGQSISQVSRTSLRQQTAYVGQDVYLFRATIGANIAFGKAGATEQEIVA
                     AAKAACAHEFIMGFPLGYDTPVGEHGTQLSGGQRQRIAVARALVKNAPIILLDEATAA
                     LDSESEKQVQEAIEHLCQNRTTIVIAHRLHTIMHADAILVVEGGEIVERGRHDDLLRR
                     GGRYASFFRLQQREAGPLALAPISATA"
BASE COUNT          468 a          801 c          864 g          494 t
ORIGIN      
        1 tctcgcttgc gcgggctgga aagctgcaat gaagcagggg aaccggattg cttcggattc
       61 attgcagcct cgagcgaaga acgcgaaatt ctcctagctg atagccgaac ggcccggagc
      121 tgtaaattcg tcagcattcc cgaagtttcg cagagccggg accctcgcgc caatgatgtt
      181 ggtccagatc ggccccggct cagcggcgcc ccgcttgcgc gctgcgccgc gtccgggcac
      241 gaaagccggc tattccggct ggccgatcgt gaccttgagg gtgccgatgc catcgacccc
      301 gcattcgatt ctgtcgccgg gcgagagtgc ggcgaccccg gccggcgttc ccgtcatgat
      361 gatgtcgccg gcgccgagcg tgacctgctc cgacagtttg gcgatgattt cgggtacgct
      421 ccagatcagt tcggtgagat cgcccttctg ccgttcggtg ccgttgaccg agagccagat
      481 cttgccgttt gccggatggc cgattttcga ggccggctgc agcgggccgc agggcgcgga
      541 atggtcgaac gatttcccga cctcccatgg ccgctccttc ttgcgcgcgg ccagttgcag
      601 gtcgcgccgc gtgaggtcga tgccgacgcc atagccgtac acatggtcga gcgccttgtc
      661 cgagggaatg tcgaggccgc cgctcttcag cgcgacgatc aattcgactt cgtgatgcaa
      721 atccttggtc agcggcgggt aggggatggt ggcgccgtcc ggctcgatca tgtcggcgtg
      781 cttggcaaag aagaacggcg gcgcccgctc gtcatttccc atctctctga tgtgttcgag
      841 atagttgcgt ccgacgcacc agatgcggcg aaccggaaag gactttgtgt cgcctgcgac
      901 cggaatcgaa ggctgtggca gggccggaat gacaaaggat gtggcgctca tggaaaactc
      961 tcaatggagg ctgaaaacaa ggcgacgatg atcgcgggca gggaaggatc gcggcgatct
     1021 tatgccgtcg cgctgatcgg cgccagagcg agaggccctg cttcccgctg ctgcaggcgg
     1081 aagaacgagg cgtagcgtcc gccgcggcgc aggagatcgt catgccgccc gcgctcgacg
     1141 atctcgccgc cctcgaccac cagaatggca tcggcgtgca tgatggtgtg caggcgatgg
     1201 gcgatgacga tggtggtgcg gttctggcag agatgctcga tcgcctcctg cacctgcttt
     1261 tcggattccg aatcgagcgc ggcggtggct tcatcgagca ggatgatcgg cgcattcttc
     1321 accagtgcac gggcgacggc gatgcgctgg cgctggccgc cggacaattg ggtgccgtgc
     1381 tcgcccaccg gcgtgtcgta gccgagcgga aagcccatga tgaattcgtg cgcgcaggcc
     1441 gcttttgccg cagccacgat ctcttgttcc gtggcaccgg ccttgccgaa cgcgatgttc
     1501 gcaccgatgg tggcgcggaa cagatagacg tcctgcccga cataggcggt ctgctgacgc
     1561 agcgacgttc gcgatacctg cgaaatcgac tgtccgtcga tcaggatgtc gccgccggtt
     1621 gcttcataaa accgcagcag cagcgccagc accgtcgatt tgccgccgcc ggaggggccg
     1681 accaaggccg tgactttgcc gggctcggcc acgaagctca tgcggttgag gaccggctcg
     1741 ttgagacgat aggcgaagct tacgtcgcgc aactcgaccc gcgcgtcggt cagcttcagc
     1801 gccggcctgt cgtcgtcgcc gggctcgctg gccgggttgt cgacgatttc cagcaacttg
     1861 cgggcgccga tcagattgct gttcaactcg atattcagcc gtgccagccg cttggccggc
     1921 tcgtaggcca gcaggaacgc ggtcaggaac gaaaagaact ggcccggcgt ggcgcccatc
     1981 gcgaccacgc tatagccgcc atacatcaat ccgcccgcga tcgcgaaccc gccgagcgtt
     2041 tccatcaacg ggctcgaacg attggaaacc cgcgccatct tgttggcgtt gcgttcgacc
     2101 gcggcgatgc tggcgtcgat acgatcgcgc atggtctgtt cgagcgtgaa tgccttgacg
     2161 gtgcgaatgc cctgcagcga ttcctgcatg gtttcgagaa tgtcggcggt accggtgaac
     2221 tggttgtgcg cgaggccctt gatccgcttc accagcttgc gcagcaccag catggccggc
     2281 ggcgccacca ggaagccgag cagcgccatg tatggatcct gcatcagcat gacggcgaca
     2341 agtgcgatca gtgacagcac atcgcgtccc acggcattga tcagcaggtt gagaacctgg
     2401 gtgacggaat tcgcgccgga ggtcagccgc gccagaaatt cggatgaatg ccgttccgag
     2461 aagaaagcga cgctttcgtt catcagcttg gcgaacagcc gccgctggtt gttggcgagg
     2521 atcgcgttgc tgatctgtga caggatcacc gagtgaccgt aggtcgccgc gcccttgagc
     2581 atgaagatca ggatggtcac ccccgacagg atcgcgatgc cgcggac
//
LOCUS       DATNOI010000505         6111 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1919285,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000505 DATNOI010000000
VERSION     DATNOI010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1919285"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VN968_20315"
     CDS             complement(<1..1031)
                     /locus_tag="VN968_20315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HXL31614.1"
                     /translation="MTTNTKFRGGSKEAPTEPFKRAVTACLRAIAKTPELEVTFAAER
                     PGLAPGKARLPEPARKMTRRDAAIVRGHADSIALKLACHDPKVHRKLMPGNPQARGVF
                     EAVEQARVEALGSRRMAGVAKNLTAMLDDHFHRGKFDEITDRADAPLSDALAMLVRER
                     LTGLAPPAAARKMVDLWRPLLEDKIGTRLDRLDRLAEDQVKFGDAVHDLLSAIDLGDE
                     RNAEADEDENQDDNRDGENDQSGAEGSPDSDAAQEMSADQAQASTDEMSESAMESAQA
                     STSDTFDDGELGDDETPGEATRPKSRGGNEPRGPEYHAFAPKFDEVIAAEDLCDHDEL
                     ERLRSYLDK"
     gene            complement(1041..1451)
                     /locus_tag="VN968_20320"
     CDS             complement(1041..1451)
                     /locus_tag="VN968_20320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL31615.1"
                     /translation="MPAISVERTIGATKRAVLGGLLRYNAEKMGKQKYRRFAISLRDD
                     GAIVGGIVGEVWTTVLFIQFFWIDEQFRGKGHGTALIEQIEKEARRFGAVRSYLDTMS
                     VQAPGFYRACGYEAFGAIDGYPGGVKRHWFTKAL"
     gene            complement(1456..2454)
                     /gene="cobS"
                     /locus_tag="VN968_20325"
     CDS             complement(1456..2454)
                     /gene="cobS"
                     /locus_tag="VN968_20325"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HXL31616.1"
                     /translation="MTTAAMTKVQESTGLPDMKVSVRQVFGIDSDLEVPAYSEVDPHV
                     PDVDSDYRFDRATTLAILAGFAKNRRVMVTGYHGTGKSTHIEQVAARLNWPCVRVNLD
                     SHISRIDLVGKDSIVVRDGMQVTEFRDGILPWALQHNIALVFDEYDAGRPDVMFVIQR
                     VLEVSGRLTLLDQNKVIKPHPAFRLFSTANTVGLGDTSGLYHGTQQINQGQMDRWSIV
                     TTLNYLAHDEEVEIVLAKAHHYRTQEGRDIVNKMVRLADLTRNAFANGDLSTVMSPRT
                     VITWAENAEIFTDIGFAFRVTFLNKCDELERPLVAEFYQRCFNAELAESSVNVAMS"
     gene            2762..3436
                     /locus_tag="VN968_20330"
     CDS             2762..3436
                     /locus_tag="VN968_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXL31617.1"
                     /translation="MASFIDPLIAFVSAHAWLAYLTLFLAALLEAVPIAGSVIPGSTI
                     ILALSALVPGGDLRLEWVLVAAIAGALLGDGTAFWIGHRTQREILSSWPMTNYPRVVA
                     QSEAFFRRWGALAVFFARFVPPIRAFVPVTAGALGMTPLRFYAVNIPAILLWAPAHVL
                     PGVLAISALHRYAGIPHHTGVGKHYWMLSVLGGALIVALAVWAIRRRKGGGIIEPATA
                     AARKDP"
     gene            complement(3476..4699)
                     /locus_tag="VN968_20335"
     CDS             complement(3476..4699)
                     /locus_tag="VN968_20335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase family protein"
                     /protein_id="HXL31618.1"
                     /translation="MKKPAALYLSAREASAELAISPATLYAYVSRGLIRSEPSSDSRS
                     HRYRAEDVRGLKERRAPSPEPRGLRNFDADLPVMDSSISTITEDGPIYRGVNCIELAE
                     TDTLEHAATLLWDVTGVDPFSQDNCPQVSGEMRSVADAARHAAPIDRTIAVLALAASA
                     DPRAFTRAADGRAMVGGRILRLVVATMLNGTSSPEPLHLQIAKAWAPDNKRAADLIRR
                     ALVLLADHELNASAFTVRCAASTGLNLYDAMIAGLVALKGPRHGGAGVLAAQLVKMLV
                     DRDVAPVIRERVALGERFAGFGHGVYKHGDLRAHALLAALARVDAAEKLTTEIPERIL
                     EATGEFVNIDYALAVLVHTLGLPPGNELVLFAMARTVGWIAHASEQLRHGGLIRPRAR
                     YIGPAPGRSPAFNRP"
     gene            4772..5863
                     /locus_tag="VN968_20340"
     CDS             4772..5863
                     /locus_tag="VN968_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase/methylcitrate synthase"
                     /protein_id="HXL31619.1"
                     /translation="MNMQLSKSPIGLDGIPAAETVLSHVDGERGELIIAGERVGDLVR
                     KTGFEGVTARLWSGGTGQPTAEATVRAALGAARERTFARLPDLLGITRGLSIVDGFRA
                     AIAGLRAENGLPHEATIVGAFPVIAGALVQRARGGEPIAPDPTVSHAADTLSMMLNRK
                     PDAREVAALDAYFVTVCDHGMNASTFTTRVVASTQADLFAAVTAGYCALTGPLHGGAP
                     EPVLEMLDAIGTSEHIKPWVDSALARGERLMGFGHRIYRVRDPRADVLKGAIERLADG
                     GAGLPFAGEVEAYIRAALRRKNPDRPLDTNVEFFTAILLDALKIPRQAFTPIFAVARA
                     AGWTAHAREQQRGGRLIRPSSAYIGAMPD"
BASE COUNT         1041 a         1978 c         1966 g         1126 t
ORIGIN      
        1 tgcttgtcga gataggagcg cagccgctcc agttcgtcat ggtcgcagag atcttcagcc
       61 gcaatgacct cgtcgaattt cggcgcaaag gcgtgatatt ccggcccgcg cggctcattg
      121 ccgccgcgag acttcggccg cgtcgcctcg ccaggcgtct cgtcgtcgcc aagttcgccg
      181 tcgtcaaacg tgtcgctggt agaggcctgc gcgctttcca tcgcgctctc ggacatctcg
      241 tcggtcgagg cctgcgcctg gtcggcgctc atttcctgcg cggcgtcact gtcgggcgag
      301 ccctcagcgc cggactggtc gttctcgccg tcgcggttgt cgtcctggtt ctcgtcctcg
      361 tcggcttccg cgttgcgttc gtcgccgaga tcgatcgcgg acaacaaatc atgcaccgcg
      421 tcgccaaatt tcacctggtc ctcggcgagc cgatcgagcc ggtcgagccg cgtgccgatc
      481 ttgtcttcca gcagcggacg ccacaggtcg accatcttgc gggcggcggc cggcggtgcg
      541 agcccggtca ggcgttcgcg caccagcatc gccagcgcat ccgatagcgg cgcgtcggcg
      601 cgatcggtga tctcatcgaa cttgccacga tggaaatggt cgtcgagcat cgcggtgaga
      661 tttttcgcaa cccccgccat gcgccgcgag ccgagcgcct cgacccgcgc ctgttcgacc
      721 gcctcgaaca cgccgcgcgc ctgcggattg cccggcatca gcttgcggtg cactttcgga
      781 tcgtggcagg cgagtttgag cgcgatcgaa tcggcgtggc cgcgcacgat agcggcgtcg
      841 cgccgggtca tcttgcgcgc cggctccggc agccgcgcct tgccgggcgc gaggcctggg
      901 cgctcggccg cgaacgtgac ttccagctcc ggagttttcg cgatcgcgcg caggcaggcg
      961 gtcaccgcgc gcttgaacgg ctcggtcggc gcttccttcg agccgccgcg gaattttgtg
     1021 ttggtggtca tcgccagtcc ttacagtgcc ttggtaaacc agtgacgctt cacgccgccg
     1081 ggatagccat cgattgcacc gaacgcctca tacccgcatg cccggtagaa gcccggggcc
     1141 tggacgctca tcgtgtcgag ataggatcgc accgccccga accgccgggc ctccttctca
     1201 atctgttcga tcagcgccgt cccgtgaccc tttccacgaa actgctcgtc gatccagaag
     1261 aactggatga acagcacggt cgtccagacc tcaccaacaa ttccgccgac tatcgccccg
     1321 tcgtcacgca gcgagatggc gaagcggcga tatttctgct tccccatctt ctcggcgtta
     1381 tagcgaagca gtccgccaag caccgcgcgc ttggtcgctc caatcgtgcg ctcgacggaa
     1441 atcgccggca tcgcatcagc tcattgcaac gttcaccgag gattccgcga gctcagcgtt
     1501 gaagcagcgc tgatagaatt cagcgaccag cggccgttcc agttcgtcgc acttgttgag
     1561 gaaggtgacg cggaaggcaa agccgatgtc ggtgaaaatc tcggcgttct cggcccaggt
     1621 gatgaccgtg cgcgggctca tcaccgtcga cagatcgccg ttggcgaagg cgttgcgggt
     1681 gaggtccgcg agccgcacca tcttgttgac gatgtcgcga ccctcctggg tgcggtagtg
     1741 atgcgccttg gccagcacga tctcgacttc ctcgtcatgg gcgagataat tcagcgtggt
     1801 gacgatcgac cagcggtcca tctggccctg gttgatctgc tgggtgccgt gatagaggcc
     1861 ggacgtatcg ccgaggccga ccgtgttggc ggtcgagaac agccggaacg ccggatgcgg
     1921 tttgatcacc ttgttctggt cgagcagcgt caggcggccg gagacctcga gcacacgctg
     1981 gatcacgaac atcacgtcgg gacggccggc gtcgtattcg tcgaacacca gcgcgatatt
     2041 gtgctgcagg gcccagggca gaatgccgtc gcggaactcg gtgacctgca taccgtcacg
     2101 caccacgatg gaatccttgc ccaccagatc gatacggctg atatggctgt cgaggttgac
     2161 gcgcacgcac ggccagttca gccgcgccgc gacctgctcg atatgggtgg atttgccggt
     2221 gccatgatag cccgtgacca tgacgcggcg gttcttggca aacccggcga gaatggcgag
     2281 cgtggtggca cgatcgaacc gataatccga atcgacgtcg ggcacatgcg gatcgacttc
     2341 ggagtaagcc ggcacttcaa gatcgctatc gataccgaac acttgccgga ccgacacctt
     2401 catgtcgggc aaaccggtgg attcctgcac tttggtcatg gcggcggtcg tcatcaatcc
     2461 tccgaggtct cggacgctcc cgaaaccaga tttgcggcat ggctgcggat gggggctagg
     2521 ctgaacctag cagagagcaa gggccggcag aagcccgcgg cacaatcaaa gttccaccgc
     2581 gttatcatct agataggtgc tggccccgtt ttttggaggg ctgccctgcg aatcacgccg
     2641 cactttcgcc gggacaattg gccacccctc gttcgagggt gccgatggca cgttccactc
     2701 tgccgataag atatgttagg tctgggcccc gaccggacag caataccagc acaggaatct
     2761 tgtggcttcc ttcatcgatc ccttgattgc gttcgtctcg gcacatgcgt ggcttgccta
     2821 tctcacgcta ttcctcgccg ccctgctgga agcagttccc atcgcaggct cggtaatccc
     2881 gggctcgacc attatcctgg cgctgagcgc gctggtaccc ggcggcgacc tgcggctcga
     2941 atgggtgctg gtggccgcga tcgcaggtgc gttgctgggc gatggaacgg cattctggat
     3001 cggacatcgc acccagcgcg aaatcctgag ctcctggccg atgacgaatt acccgcgcgt
     3061 ggtggcgcag agcgaagcgt tctttcgtcg ctggggcgcg ctggcggtgt tcttcgcccg
     3121 atttgtgccg ccgatccgcg ccttcgtacc ggtcacggca ggcgcgttgg gcatgacgcc
     3181 gctgcgcttt tacgccgtca acatcccggc gatcctgcta tgggcgcccg cgcatgtgct
     3241 gccgggcgtg ctggcgatat ccgcactgca ccgctacgcc ggaattccgc atcataccgg
     3301 tgtaggcaag cactactgga tgttgtcggt gctgggcggt gcgttgattg tcgcgctggc
     3361 ggtctgggcg atccgtcgcc gaaagggcgg aggcattatc gagccggcaa cggccgccgc
     3421 tcgcaaggat ccctgacggc tcaggatccg gcgctcattc aaattctgca aatccttacg
     3481 gcctgttgaa agccgggctg cgccccggcg ccggaccgat atagcgcgcg cgggggcgga
     3541 tcagtccgcc atgccgcaat tgctcgctcg catgcgcgat ccatccgacg gtccgcgcca
     3601 tcgcaaacag caccagttcg ttgccgggcg gcaggcccag cgtatgcacc agcaccgcga
     3661 gcgcgtagtc gatattgacg aattcaccgg tcgcttccag gatccgctcc ggaatctcgg
     3721 tggtgagttt ctccgccgca tcgacccgcg ccagcgctgc cagcagggca tgcgcgcgca
     3781 gatcgccgtg cttgtagacg ccgtgaccga agcccgcgaa acgttcgcca agcgcaacgc
     3841 gctcccggat gaccggggcg acatcgcgat ccaccagcat cttcacgagc tgcgcggcca
     3901 ggacgccggc gccgccatgt ctggggcctt tcaacgccac gagaccggcg atcatggcat
     3961 cataaagatt caatccggta gacgccgcgc accgcacggt aaaggccgag gcgttcagtt
     4021 catggtccgc cagcagcacc agcgcacggc ggatcaaatc ggcggcgcgc ttgttgtccg
     4081 gcgcccaggc cttggcgatc tggagatgaa gcggttccgg cgacgatgtg ccattcaaca
     4141 tcgtggcaac caccagacgc aggatgcggc cgccgaccat cgcgcgcccg tcggcggcgc
     4201 gggtaaaggc gcgtggatcc gcgcttgcgg ccagcgccag cacggcgatg gtgcgatcga
     4261 tcggtgcggc atggcgcgcg gcgtcggcga cgctgcgcat ttcgcccgat acctgcgggc
     4321 aattgtcctg ggaaaacgga tcgacgccgg tcacatccca cagcaacgtc gcggcatgtt
     4381 cgagcgtatc cgtctccgca agttcgatgc agttcacgcc gcggtaaatc ggcccatcct
     4441 cggtgatggt cgaaatcgac gaatccatca ccggcagatc ggcgtcgaaa tttctgaggc
     4501 cgcgcggctc gggcgacggc gcgcgccgtt ccttcagccc gcggacgtcc tccgcccgat
     4561 agcgatggct tcgtgaatcc gacgagggct cggagcggat caatccgcgg ctgacatagg
     4621 cgtacagcgt ggcgggcgag atcgcgagtt ctgccgaggc ctcgcgggcg gagaggtaaa
     4681 gtgccgcggg ttttttcata ttgatttaca taatcaagat tgatcaatct gtcgagaggg
     4741 tcgacctttg aggcttcaaa ggagaccgac catgaacatg caactttcca agagcccgat
     4801 cgggctggac ggcattcccg ccgccgaaac ggtgctcagc catgtcgatg gcgaacgtgg
     4861 cgaactaatc atcgccggcg aacgcgtggg tgacctcgtg cgcaaaaccg gcttcgaggg
     4921 tgtgaccgcg cggctgtgga gcggcggaac cggccagccg acggctgagg ccaccgtgcg
     4981 cgccgctttg ggcgcggccc gcgaacgcac ctttgcccgg cttccggact tgctgggaat
     5041 tacgcgcggc ctgtcgatcg tcgatggctt ccgcgccgcc atcgccggat tgcgcgccga
     5101 aaacgggttg ccgcatgagg ccaccattgt cggggcgttt ccggtgatcg ccggcgcgct
     5161 ggtgcagcgc gccagaggcg gtgaacccat cgcgcccgat ccgaccgtca gccacgccgc
     5221 cgatacgctg tcgatgatgc tcaaccgcaa acccgacgcg cgagaggtgg cggcgctgga
     5281 cgcctatttc gtgacggtgt gcgatcacgg catgaatgca tcgaccttca ctacccgcgt
     5341 cgtggcctcg acccaggccg acctgttcgc ggccgtgacg gcgggatatt gcgccctgac
     5401 gggaccgctt catggcggcg caccggagcc ggtgctggaa atgctggacg ccatcggcac
     5461 cagcgagcac atcaagccct gggtcgacag cgcgctggca cgcggcgaac ggctgatggg
     5521 cttcggccac cgcatctatc gcgtccgcga tccgcgcgcg gatgtgctca agggcgccat
     5581 cgaacggctc gccgatggcg gcgccggtct tccgttcgcg ggcgaggttg aggcctatat
     5641 ccgcgcggcg ttgcggcgga aaaacccgga ccggccgctc gacaccaatg tggagttttt
     5701 caccgcgatc ctgctcgacg ccctgaaaat cccgcggcag gctttcacgc cgatcttcgc
     5761 ggttgcccgc gccgcgggat ggaccgcgca tgcccgcgag cagcagcggg gcggtcggct
     5821 gatacggcca agttcagcct atatcggggc gatgccggat tgatagcagg gcttgacgat
     5881 ctcgctcctc atggtgagga ggcgcccttg cgccgtctcg aaccatgagg aagattgagt
     5941 gtcgtccttc gcgacgcggc gaagacgccc ctctcagagc ttgtgaagaa tacgacctca
     6001 tcctgaggag cttgcgaagc aggcgtctcg aaggatggac acaacgcatg gactcgcggc
     6061 catccttcga gacgcggcca agaggccgct cctcaggatg aggtctccgc g
//
LOCUS       DATNOI010000506         4048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1104842,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000506 DATNOI010000000
VERSION     DATNOI010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104842"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1087)
                     /locus_tag="VN968_20345"
     CDS             complement(146..1087)
                     /locus_tag="VN968_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HXL31620.1"
                     /translation="MQLTGIHHLTAISAKPRQNLAFYTGLLGMRLVKKTVNQDDVSAY
                     HLFYADGNANPGTDLTFFDFPAAPERRGTNSISRTGLRVAGEKSVGYWRDRLKKAGGH
                     TGEIIEVDGRLTLPFEDGEGQRLVLVDDGGAGPASPWEKSPVPAEHQIRGLGPIVLTV
                     HDLARTEQVLTGVMNMRPVRDYAAHGAQIHVFAMGEGGPAAELHVIESADLQEARQGA
                     GGVHHVAFRTPDDEQYHAWTRRLNASGIRNSGEIDRFYFRSLYFREPNGILLEIATDG
                     PGFATDEPLERLGEKLALPPFLEPRRAAIEAGLKPLG"
     gene            1227..1988
                     /locus_tag="VN968_20350"
     CDS             1227..1988
                     /locus_tag="VN968_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXL31621.1"
                     /translation="MAGHLIVTDEDATRVITLRRPEKKNAITQDMYRAMSDAIDTAQN
                     NPAIRCMIITGGSGVFTAGNDLEDFLKDGTSTTDLPRASNATKFLYSLAHNVKPIIAA
                     VDGVAIGIGTTMLFHCDYVLASKTATFSTPFIHLGLVPEGASSLLMPRTMGYQRAFAT
                     LVMGRTVSADDARVAGFVNVVVAPGHTEAEARKVAREICALPAEAVAISRKLLKLPPE
                     DLTRRIDQEGHLFGERMRSKEAVAAFKAFFSRKKA"
     gene            2036..3703
                     /locus_tag="VN968_20355"
     CDS             2036..3703
                     /locus_tag="VN968_20355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HXL31622.1"
                     /translation="MRTPLLPFLAGALLLVLAPPPRAAARAAPPIDLRILAINDFHGN
                     LLPPPGGIRIADPDDNTRKITVPAGGAEHMATLVKQLRQGTRNTIFVAAGDLIGASPF
                     LSAMFHDEPTIESLSMMGLEVASVGNHEFDEGKVELLRMQNGGCHPTDNCQGPHPFLG
                     ARFHYLAASTIETASGKTIFPPYEVREFDGIRVAFVGLTLKGTPNLVSPVSVAGLEFR
                     DEADTVNALVPELKAQGIEAIVVLIHEGGFPAGDYNECPGISGPIVDIVRKFDRAVDV
                     VISGHTHQAYVCAIDGRLVTSGDKYGTIVTTIDLKLDPATRDVVSARADNFIVRTGTY
                     AKDPEQTALLASYDKFAAPIANRLAGSITQTLSRTPNDAGESPLGDIVADAQLAATSA
                     DNNGGAVIAFTNPGGVRTDIPKKEQGGVTYADVFASQPFRNQLVTLTLTGMQIKNMLE
                     QQWLDPKRPRVLQVSKGFNYAWDNAKPYGDRIADRMSLNGQRIDPATSYRVTVNNYLA
                     VGGDGFTVLKEGSAQQFGVYDVDALYAYFLANSPIGPAPADRIVRMN"
     gene            3801..>4048
                     /locus_tag="VN968_20360"
     CDS             3801..>4048
                     /locus_tag="VN968_20360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HXL31623.1"
                     /translation="MTTLVLTHSACLGHATPPGHPERADRLRAVGEVLAENRFSRLVR
                     GEAPEGSLDSVTLCHNEHYVEELRHIAPSSGLIYLDGDT"
BASE COUNT          762 a         1307 c         1240 g          739 t
ORIGIN      
        1 ggagcgcgct cttgcgcgcg tctcgaagga tggccgctag cccatgcgtt gtgtccatcc
       61 ttcgagacgc ttgcttcgca agctcctcag gatgaggtcg gagatgcaac tgttgtcgct
      121 ggccgaaagc ctccgcgccg atagttcacc cgagcggctt cagccccgct tcgattgcgg
      181 cgcggcgcgg ttccagaaac ggcggcagcg ccagtttctc gccgagcctt tccagcggct
      241 cgtcggtggc aaagccgggg ccgtcggtgg cgatctcgag caggatgccg ttgggctcgc
      301 ggaaataaag gctgcgaaaa tagaaccggt cgatctcgcc gctgttgcgg atacctgatg
      361 cgttcagccg ccgcgtccag gcgtgatatt gctcatcgtc gggcgtgcgg aacgcgacgt
      421 ggtgcacgcc gcccgcgccc tgccgcgctt cctgcaaatc cgcactctcg atgacgtgca
      481 gttccgcggc cggaccgccc tcgcccatcg caaacacatg gatctgcgcc ccgtgcgcgg
      541 catagtcgcg cacggggcgc atgttcatca cgccggtcag cacctgctcg gtgcgggcga
      601 gatcgtgcac ggtgagcacg atcggaccaa ggcccctgat ctggtgctcc gccggcaccg
      661 ggctcttctc ccacggcgag gctggccccg cgccgccatc atcgaccagc accagccgct
      721 ggccctcacc atcctcgaac ggcagcgtca gacggccgtc gacctcgatg atctcaccgg
      781 tatggccgcc cgctttcttg aggcggtccc tccagtagcc aacgcttttt tcgccggcga
      841 cgcgcaatcc ggtgcgggaa atgctgttgg tgccgcgccg ctccggtgct gccgggaaat
      901 cgaagaatgt aaggtcggtg ccgggattgg catttccgtc ggcgtagaac agatgatagg
      961 cgctgacgtc gtcctgattg acggtcttct tcaccagccg catgccgagc aggccggtat
     1021 agaacgccag gttctgccgt ggtttcgcgg aaatcgctgt cagatggtga atcccggtca
     1081 actgcatcga attatccttt aatccttcag accccttccc ggggaaaatc ggcggaggca
     1141 ttggggcccc atcgcggatc atgtaggata tagtcgggag actcgccagc ggcccccttt
     1201 gccgcctggg tttcaaggac agcttcatgg ccggacacct gatagtcacc gacgaggacg
     1261 cgacgcgggt gatcacgctg cggcggcccg aaaagaagaa cgcgatcacg caggacatgt
     1321 atcgcgccat gagcgatgcg atcgacaccg cgcagaacaa tccggcgatc cgctgcatga
     1381 tcatcaccgg cgggtcgggc gtattcaccg ccggcaacga tctggaagat tttctgaaag
     1441 acggcacctc cactaccgac ctgccgcgcg cgtccaacgc gacgaagttt ctctattcgc
     1501 tggcgcacaa cgtcaagccg atcatcgccg cggtcgacgg cgtagccatt ggcatcggca
     1561 ccaccatgct gttccactgc gattacgttc tcgccagcaa gaccgcgaca ttctccacgc
     1621 cgttcatcca tcttgggctg gtcccggaag gcgcctccag cctgctgatg ccgcgcacca
     1681 tgggctacca gcgtgcgttc gccaccttgg tgatgggacg cacggtcagc gccgacgacg
     1741 cgcgcgtcgc cggcttcgtc aacgtggtgg tggcgcccgg ccacaccgag gccgaggcgc
     1801 gcaaggttgc gcgcgagatc tgcgcgttgc cggccgaagc ggtggcgatt tcgcgcaaac
     1861 ttctgaaact gccgcctgaa gacctgaccc gcaggatcga ccaggaaggc cacctgtttg
     1921 gcgagcggat gcgctcgaag gaagcggtgg ccgcgttcaa ggcattcttt tcacgcaaga
     1981 aggcctgagt cgaagcatcg tgaaatcgtt atgctggatg ctctattata gagtcatgag
     2041 aacgccgctc ttgccattcc tcgcgggcgc gctgcttctg gtgctggctc cgcccccgcg
     2101 cgcggccgcg cgggctgcgc cgccgatcga tctgcgaatt ctcgcgatca acgatttcca
     2161 cggcaatctt ctgccgccgc cgggcgggat caggatcgcc gatcccgacg acaacaccag
     2221 gaagatcact gtgccggcgg gcggcgccga acacatggcg acgctggtca agcaactgcg
     2281 ccagggcacc aggaacacga tttttgtcgc ggccggcgac ctgatcggcg ccagcccgtt
     2341 tctgtcggcg atgtttcacg atgagcccac catcgaatcg ctgtcgatga tggggctgga
     2401 ggtagcctcg gtcggcaatc acgaattcga cgagggcaag gtcgaattgc ttcggatgca
     2461 gaatggcggc tgtcatccca ccgacaattg ccaggggccg cacccgttcc ttggcgcaag
     2521 attccactac cttgccgcaa gcacgatcga gaccgcttcc gggaagacga tatttcctcc
     2581 ctacgaagtc agggagttcg atggaattcg cgtcgccttc gtcggcctga cattgaaggg
     2641 aacgcccaac ctcgtctccc cggtgagcgt cgcaggtctc gaattcaggg acgaggctga
     2701 cacggtgaat gcgctggtgc cggagctcaa ggcgcagggc atcgaggcca tcgtcgttct
     2761 gatccacgaa ggtggcttcc cggccggcga ctataatgaa tgcccgggca tttccggacc
     2821 gatcgtcgac atcgtcagga aattcgatcg tgcggtcgac gtggtgatca gcggtcacac
     2881 ccatcaagcc tatgtctgcg cgatcgatgg acgacttgtc accagtggcg acaagtacgg
     2941 caccatcgtc accacgatcg atctgaagct cgatcccgcg acccgcgacg tcgtcagcgc
     3001 cagggctgac aacttcattg tccgcaccgg tacttatgcc aaggatccgg aacagaccgc
     3061 gctgcttgcc tcctacgata agttcgcagc cccgatcgcc aaccgcctgg cgggctcgat
     3121 cactcaaacg ctgtcgcgca cgccaaacga tgcaggcgag agcccgctcg gcgatatcgt
     3181 cgccgatgcc cagctggccg cgaccagcgc cgacaacaac ggcggagccg tgatcgcatt
     3241 caccaatccg ggcggcgtcc gcaccgatat tccgaagaaa gagcaaggcg gcgtgaccta
     3301 cgccgatgta ttcgccagcc agccgttccg gaatcagctg gtgacgctga cgctgacggg
     3361 aatgcagatc aagaacatgc tcgaacagca atggctcgat ccgaagcggc cgcgggttct
     3421 gcaggtttcg aagggcttca actatgcatg ggacaacgcc aagccctatg gcgaccgcat
     3481 agccgatcgc atgtcactga acgggcaacg catcgatccc gcgaccagct atcgcgtgac
     3541 ggtgaacaat tatctcgcgg tcggcggcga cggtttcacg gtattgaagg aaggctcagc
     3601 acagcaattc ggcgtctacg acgtcgatgc cttgtacgcc tatttcctgg ccaacagccc
     3661 gattgggccg gctccagccg atcgcatcgt gcgcatgaac tgaattacaa agccatatcg
     3721 gcggcacggc tctttccgag gccgcgatga ccccttagat tagcctgtcc tcacaagcgc
     3781 ctccggcgcc agggtattgc atgacgacgc ttgttctcac ccattcggcg tgtctcgggc
     3841 acgccacgcc gccggggcat ccggagcgcg ccgaccggct gcgcgcggtc ggcgaggtgc
     3901 tcgcggagaa ccgcttcagc aggctggtgc gcggcgaagc gccggaaggc agtctggatt
     3961 cggtcacgct gtgtcacaac gagcattacg tcgaggaact ccggcacatc gcgccatcga
     4021 gcggtctgat ctatctcgac ggcgatac
//
LOCUS       DATNOI010000507         4361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2535468,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000507 DATNOI010000000
VERSION     DATNOI010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2535468"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VN968_20365"
     CDS             <1..523
                     /locus_tag="VN968_20365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HXL31624.1"
                     /translation="AGGYSLGYQTQVVLPLRIVAKNADKPVTLRADINYAVCEKLCIP
                     VEASAELAITSVASTEDSALFAALDTVPKPANVGDPNPLTIRDVKREGKSTVLVDVAV
                     PDTRAVDLFVEGPTPDWALPVPRLLEHSPPGVKRFAFELEGLPPGTNPDGAALKLTLV
                     GGDRAYEFNINLD"
     gene            complement(730..1518)
                     /locus_tag="VN968_20370"
     CDS             complement(730..1518)
                     /locus_tag="VN968_20370"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HXL31625.1"
                     /translation="MGTLTGKTAVVTGSTSGIGLAYARAFAGAGANIVLNGMGAPADI
                     EKERAGIEADFKVKAVHSPADMTKPAQIAEMVALGEKTFGSVDILVNNAGIQFVSPIE
                     EFPPEKWEAIIAINLSSAFYGIRAAVPGMKKRGWGRIINTASAHSLVASPFKAAYVSA
                     KHGIAGLTKTVALELATFKITCNCISPGYVWTPLVEKQIPDTMKARNMTKEQVIRDVL
                     LEAQPTKEFVTSEQVAALALFLCGDDAAQITGTNLSIDGGWTAE"
     gene            complement(1524..2702)
                     /locus_tag="VN968_20375"
     CDS             complement(1524..2702)
                     /locus_tag="VN968_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3734 domain-containing protein"
                     /protein_id="HXL31626.1"
                     /translation="MTDQSNPAPAKTPATAQRVLVLQGGGALGSYQAGAYQALCHFDF
                     EPEWIAGISIGAINAAIIAGNPREKRIGRLKEFWEMVSAPVPWNPVTKSDRGRLLFNE
                     TSAALIATFGVPGFFTPRFPPAPLWPQGSPQSESYYDTAPLRKTLERLVDFDRINDLK
                     TRLSVGAVSVTTGNFKYFDNFEFKKLGKKIEPEHIMASGALPPGFPSIVIEGEHYWDG
                     GIASNTPLDYVLDEGGRNDLLIFQVDLFSARGPLPVSLLDAAEREKDIRFSSRTRMNT
                     DKNRQIHNARKAVRDLISKLPDDLKNDPSVEFLREAAKENTVTVVHLIYRSKNYESSS
                     KDYDFSHVGMVEHWGAGVRDVHLSMRHKDWLERPQSGETMVTYDLAGDSPETLDGKKE
                     "
     gene            complement(2796..3299)
                     /locus_tag="VN968_20380"
     CDS             complement(2796..3299)
                     /locus_tag="VN968_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HXL31627.1"
                     /translation="MRAAVAIIGLLALGGCAGDVSTIDQPSMYISMANGGAKLDPQAA
                     ASMISLYRQNNGLGGVAVDPDLMKLAESQSQAMASHNKLDHDVKAPLARRLNASGYPA
                     TLAVENVSAGYHTLAEAFSGWRDSPPHRANMLKNGVTKLGIAASYAPNTKYKVFWTLI
                     LASSETG"
     gene            complement(3482..>4361)
                     /locus_tag="VN968_20385"
     CDS             complement(3482..>4361)
                     /locus_tag="VN968_20385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HXL31628.1"
                     /translation="EVSFDGENALARGASERHVGFVFQHYALFRHMTVFENVAFGLRV
                     QPRAVRKDEAGIRARVKELLDLVQLDWLSNRYPSQLSGGQRQRIALARALAIEPRILL
                     LDEPFGALDAKVRKELRQWLRSLHSEIHVTSIFVTHDQEEALEVANRVVVMDKGRIEQ
                     IGTPGEVYDNAATAFVHGFIGESIVLPVEVCDGCVRLGGKSLSISPDGVGSGASKLFV
                     RRHDMQVGPAGSGALDGAVRHVRTFGPIQRAEVALSATGGETVIEIDAPRDRELQIGE
                     IVGLQPRRYRIFAAQD"
BASE COUNT          821 a         1365 c         1332 g          843 t
ORIGIN      
        1 agccggcggc tattcgttgg gctatcagac ccaggtcgtg ctgccgctgc ggattgtcgc
       61 caaaaacgcc gacaagccgg tgacgcttcg cgccgatatc aactacgcgg tgtgcgagaa
      121 actctgcatt cccgtcgagg ccagcgccga gctcgccatc accagcgtgg cgagcaccga
      181 ggacagtgcg ctgttcgccg ccctcgacac cgtgcccaag ccggccaatg tcggagaccc
      241 caacccgttg accatccgtg acgtcaagcg cgaggggaaa tccacggtgc tggtcgacgt
      301 ggccgtgccc gacaccaggg ccgtcgatct gtttgtcgaa gggccgacgc cggactgggc
      361 gctcccggtt cccaggctgc tggaacacag cccgcccggg gtcaagcgtt tcgccttcga
      421 actcgagggc ctgcctccgg gcacgaaccc cgatggcgct gcgctcaaat tgaccctggt
      481 cggcggcgat cgcgcttacg aattcaacat caatctggac tagcgatctt cgtcattcca
      541 ggatgcctct tagcgcagcc gcggaattca taccgctggc gttcgggaag cgcagctaaa
      601 ttctctcctc gtcatgcccg ggcttgtccc gggtatccac gcctttcaga ggtagcgaca
      661 agcaagacgt agatggccgg gacaagcccg gccatgacgg cgtgatgtct taggccgtga
      721 atcctatccc tattccgcgg tccagccgcc gtcgatcgag agattggtgc cggtaatctg
      781 cgccgcgtcg tcgccgcaaa gaaacagcgc cagcgccgcg acctgctcgg aggtgacaaa
      841 ctccttggtc ggctgcgctt ccagcagcac gtcgcgaatg acctgctcct tggtcatgtt
      901 gcgcgccttc atggtgtcgg gaatctgttt ctcgaccagc ggcgtccaga cataacccgg
      961 gctgatgcag ttgcaggtga tcttgaaggt cgcaagctcc agcgccacag tcttggtgag
     1021 gccggcgatg ccgtgcttgg ccgaaacgta agccgccttg aacggcgagg ccaccagcga
     1081 atgcgcggaa gccgtattga tgatgcggcc ccagccgcgc ttcttcatgc cgggcacggc
     1141 ggcgcggatg ccataaaacg ccgacgacag gttgatcgcg atgatcgcct cccatttctc
     1201 gggcggaaac tcctcgatcg gcgacacgaa ctggatgccg gcattgttga ccaggatgtc
     1261 gaccgagccg aaggtttttt cacccagcgc gaccatctcg gcgatctgcg caggcttggt
     1321 catgtcggcg ggcgaatgga ccgccttgac cttgaaatcc gcctcgatgc cggcccgctc
     1381 cttctcgatg tcggcgggcg cacccatgcc gttgaggacg atgttggcgc cggcgccggc
     1441 gaaggcgcgc gcatacgcca gtccaatgcc gctggtcgat ccggtcacca cggcagtctt
     1501 gcctgtcaac gtacccattt cattcactcc tttttgccgt caagggtttc ggggctgtcg
     1561 cccgcgaggt cataagtgac catggtttcg ccggattgcg gccgctcgag ccaatccttg
     1621 tgacgcatcg acagatgaac gtcgcgcacc cccgctcccc agtgctcgac catgccgacg
     1681 tgggaaaaat cgtaatcctt cgaagacgat tcgtaattct tgctgcggta gatcaggtgc
     1741 accacggtga cggtgttttc cttggcggct tcgcgcagaa attcgaccga tggatcgttc
     1801 ttgagatcat cgggcaattt gctgatcagg tcgcgcaccg ccttgcgggc gttgtgaatc
     1861 tgtctgttct tgtcggtgtt catgcgggtg cggctggaaa agcggatgtc tttctcgcgt
     1921 tcggcggcat cgagaagtga aacaggcagg gggccgcgcg cactgaacag atcgacttga
     1981 aagatcagga ggtcgttcct gcctccttca tccagcacat aatcgagcgg cgtgttggat
     2041 gcgatgccgc cgtcccagta atgttcgcct tcaatgacga tcgaaggaaa gcccggcggc
     2101 agtgcgccag acgccatgat gtgctccggt tcgatcttct tgccgagctt cttgaactcg
     2161 aaattatcga aatatttgaa gttgccggtc gtcacgctca ccgcaccgac gctgagccgg
     2221 gtcttcaggt cgttgatgcg gtcgaaatca accaggcgct ccagcgtttt tcgaagcggg
     2281 gcggtgtcgt aataactctc tgattgcggg ctgccctgcg gccacagcgg cgccggggga
     2341 aaacgcggcg tgaaaaagcc ggggacgccg aaggtggcga tcaaggcggc gctggtctcg
     2401 ttgaagagca aacgcccgcg gtcgctcttc gtgacgggat tccagggtac tggcgcagag
     2461 accatttccc agaattcctt gagacgcccg attcgctttt cccgcggatt gccggcgata
     2521 atcgcggcgt tgatggcgcc gatcgagatg ccggcaatcc attccggctc gaaatcaaag
     2581 tgacacaagg cctgataggc gccggcctga taggagccga gcgcgccgcc gccttgcaga
     2641 accagcacgc gctgcgccgt cgcgggcgtc tttgctggcg ctggattgga ttgatccgtc
     2701 atgaccgccg atccaataat aaaaaacgac tgcagcgtgg cgtcagttcg cgatggcgtc
     2761 aatcggcgag gggaatgacg cggatcacgc cccggttatc ccgtctccga tgatgcaaga
     2821 atgagggtcc agaatacctt gtatttggtg tttggagcat agctcgcggc gatgcctaat
     2881 tttgtgacac catttttcag catatttgcc cggtgcgggg gcgagtcgcg ccagccggaa
     2941 aacgcttccg ccagggtatg atatccggca gacacatttt ccaccgcgag cgtcgccgga
     3001 tatcctgacg cattcaggcg tcgcgccagc ggcgccttga cgtcgtggtc aagcttgttg
     3061 tggctcgcca tcgcctgcga ctgcgattcc gcaagcttca tgaggtcggg atcgaccgcc
     3121 acgccgccaa gcccgttgtt ctgccggtag agcgatatca tcgaggcggc agcctggggg
     3181 tcgagcttgg ctccgccatt ggccatgctg atatacatgc tcggctggtc gatggtggaa
     3241 acatccccgg cgcacccgcc cagcgccaaa agcccgataa tcgcgaccgc ggcgcgcatg
     3301 atccgaaatt cccccaaaat ggattgcggt tgttgcatac caaccgtggc tgaatagtga
     3361 acggcggtcc aatttcgaag gtcattccgg ggcgaggcgg agcctcgaac ccggaatctc
     3421 gagatgacag gcgtgagatt ccgggttcgc gcttacgcgc gccccggaat gacgatcaag
     3481 actagtcctg cgcggcgaaa atgcggtagc ggcggggctg caatccgacg atctcgccga
     3541 tctgaagttc ccgatcccgg ggcgcatcga tctcgatcac ggtctcaccc ccggtcgccg
     3601 acaacgcaac ttcggcgcgc tggatcgggc cgaaggttcg gacatgccgc accgcgccgt
     3661 cgagcgcacc gctgccggcc ggcccgacct gcatgtcatg gcggcggacg aataatttcg
     3721 atgcgccaga cccaacgccg tccggcgaga tactcagcga cttgccgcca agccgcacgc
     3781 acccgtcgca aacctccacc ggcagcacaa tcgattcacc gatgaagccg tgcacgaacg
     3841 ccgtggccgc attgtcatag acctcgccgg gcgtgccgat ctgctcgatg cggcccttgt
     3901 ccatcaccac cacccggttt gctacttcga gcgcctcttc ctgatcgtgg gtgacgaaga
     3961 tcgaggtcac gtggatttcg gaatgcaacg agcgcagcca ttgccgcagt tccttccgga
     4021 ccttggcgtc gagcgcaccg aacggctcat ccaaaagcag gatgcgcggc tcgatcgcca
     4081 gcgcgcgcgc cagcgcaatg cgctggcgct gaccgcccga caattggctc ggatagcggt
     4141 tggatagcca gtcgagctga accagatcca gcaattcctt gacgcgcgcg cggatacccg
     4201 cctcgtcctt gcgcaccgcg cgcggctgca cgcgcaggcc gaaagcgacg ttttcgaaca
     4261 ccgtcatgtg gcggaacagc gcatagtgct ggaacacgaa tccgacatgg cgctcgctgg
     4321 ctccgcgggc gagcgcattc tcaccgtcga acgaaacctc g
//
LOCUS       DATNOI010000508         9985 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_720269,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000508 DATNOI010000000
VERSION     DATNOI010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9985
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720269"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VN968_20390"
     CDS             complement(<1..932)
                     /locus_tag="VN968_20390"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HXL31629.1"
                     /translation="MHPAVLNGSYDVARVREDFPALTLKVYGKPLVYLDNAASAQKPN
                     AVLDRMTEAYKSEYANVHRGLHYLANAATEAYEGGRAKVAKFLNAGRNEEIVFTRNAT
                     EAINLVASSWGEPNIKAGDEIVLSIMEHHSNIVPWHFLRERHGAVIKWVDVDDEGNFL
                     IDEFEKALTPRTKLVAITQMSNALGTIVPVKDVVKLAHARGIPVLIDGSQAAVHLAID
                     VQDIDCDFYVFTGHKLYGPTGIGVLYAKHEHLVAMRPYNGGGEMIREVARDWVTYGDP
                     PHKFEAGTPAIVESIGLGAAIDYVNSIGKERIAA"
     gene            complement(935..2260)
                     /gene="sufD"
                     /locus_tag="VN968_20395"
     CDS             complement(935..2260)
                     /gene="sufD"
                     /locus_tag="VN968_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HXL31630.1"
                     /translation="MNVVLAKTEKGHALSDIFAIAQDRLPGAGKVADHRRQAFEAYER
                     VGLPHRRIEDWKYTDLRLLMREVLPLAPAPDAAALTRAGTALKLHAIGGARRLVLVDG
                     VFAPKLSEVAGLGKGLSIRTLRDVLEAGDAALHALLFAPENSDSTVALNSAMMTDGLV
                     IEVADGAVLTQPLHIVHIASGAAPAAMFTRSVLKVGKAASATLVESYIAADGAKAYQV
                     HDSLVVAIGDGARLDHVRLIEDGREAFNISSAVLRLGAHAHFNTFGMTSGSSVSRYHA
                     VIAFAGEGSRVETNGVNLLNGRQHADTTLFLDHAVPNCASRELFRAVVDDRGHSVFQG
                     RIIVRPKAQKTDAKMMTRALLLSDEAEADNKPELEIFADDVTCGHGATTGALDESLLF
                     YLRARGLSEKEAQALLIQAFVGEAIESIAHDGLRELAISAAQRWLAARG"
     gene            complement(2282..3034)
                     /gene="sufC"
                     /locus_tag="VN968_20400"
     CDS             complement(2282..3034)
                     /gene="sufC"
                     /locus_tag="VN968_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HXL31631.1"
                     /translation="MSLLEVKNLQVRVEEREILHGLSLTVNLGEVHAIMGPNGSGKST
                     LSHVIAGKPGYEVTAGEILFKGEDLLAMAPDERAARGVFLAFQYPVEIPGVATMTFLR
                     TALNAQRKARGESEFSTPDFLKKVRSVAASLNIPQDMLRRGVNVGFSGGEKKRNEILQ
                     MALFEPSLCILDEMDSGLDIDALRVAADGVNALRSPDRAMVVITHYQRLLNYIVPDFV
                     HVMSKGRVVKSGDKELALELEASGYAQFEDAA"
     gene            complement(3197..4678)
                     /gene="sufB"
                     /locus_tag="VN968_20405"
     CDS             complement(3197..4678)
                     /gene="sufB"
                     /locus_tag="VN968_20405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HXL31632.1"
                     /translation="MAAVQETVDRVRRIDVDQYRYGFETLIESDKAPKGLSEDTVRFI
                     SAKKNEPAWMLEWRLEAYRRWLTMTEPKWARVHYPKIDYQELYYYSAPKPKKTIGSLD
                     EVDPEILKTYEKLGIPLREVEVLEGVVRPEGERRIAVDAVFDSVSVATTFQKELKQAG
                     VIFMPISEAIREHPDLVKKYLGTVVPTSDNYFATLNSAVFSDGSFVYVPPGVRCPMEL
                     STYFRINERNTGQFERTLIIADKGAYVSYLEGCTAPQRDENQLHAAVVELVTLDDAEI
                     KYSTVQNWYPGNSEGLGGIYNFVTKRGDCRGNNSKISWTQVETGSAITWKYPSCILRG
                     DNSRGEFYSIAISNGHQQVDSGTKMIHLGKNTTSRIISKGIAAGISQNTYRGLVTAHR
                     KATGARNFTACDSLLIGDKCGAHTVPYIEAKNSSALFEHEATTSKISEDVLFYCVQRG
                     LSQEEAVGLVVNGFVKDVLQQLPMEFAVEAQKLISISLEGSVG"
     gene            complement(4775..5932)
                     /locus_tag="VN968_20410"
     CDS             complement(4775..5932)
                     /locus_tag="VN968_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HXL31633.1"
                     /translation="MPDRVYLDWNATSPLRPQARAAMAAAWDFCGNPSSVHAEGRQAR
                     RLVEEARAAVAGAVGTLPRNVVFTSGGTEANALALTPGLRRGSGLPVERLLVSAIEHT
                     SVLAGGRFPTEAMGTIGVTRAGVLDLDRLRTKVEAGPPALVSVMLANNETGAVQPAAE
                     AAEIVHAAGGLMHVDAIQALGKIYFDIKALNADLVTVSGHKIGGPKGVGAVALAEGVR
                     GLEPWLRGGGQELGHRAGTENVAGIVGFGAAAKAAMGVRESDAIRLESLRNRLERGLR
                     ETTGAIVFSGDVPRLPNTTLFTVPGLKAETAVIGFDLEGIAVSSGSACSSGKVQPSHV
                     LKAMGYGANTAQGAVRLSLGWSTSEADIDRCLEAWRKLTDTLLKELRRNTA"
     gene            complement(5966..7198)
                     /locus_tag="VN968_20415"
     CDS             complement(5966..7198)
                     /locus_tag="VN968_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HXL31634.1"
                     /translation="MKRQTVSHSPHLAPERSARRVDLDWVRIGAFGLLILYHVGMLYV
                     SWGFHIKSAHRITALEPLMLVLNPWRLALLFLVSGAATRFMLGKYAVGPLLRSRSVRL
                     LIPLIFGMLVIVPPQAYDQIVESLGYPAGFPDFYARHYFAFGPQFCGPGPCILLPTWN
                     HLWFVAYLWIYTMALGVVWVAFPGLTGWVERRLVPVLSGAGLLVAPSVAFAAYRLAVL
                     PSFPSTHALFGDWYNHAVYASVFLLGFLLARAEAFWDAIERQRRLALSLAAAVFLSFL
                     VLRWTRESGAPPSLLLKLYGGIAYGCYQWFCMVAVLGFARRWLTADSGVRRYLTDAIF
                     PYYIVHQTAIIMIAHELHGRDLPAWLEASIVISGTLAVCVLTYEIVRRIAILRPLFGL
                     RVSPFEQTPVRPTAQPAQ"
     gene            7229..8212
                     /locus_tag="VN968_20420"
     CDS             7229..8212
                     /locus_tag="VN968_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HXL31635.1"
                     /translation="MSDVKNAQGGPRVEPVWDQKPPAWDEARPRGTSGGLGGISGADW
                     LLYGAVAAISLGIGVVNALSKAQDVAWRGGVYDARTQLFWELSSIVTIILVAPVLFVA
                     VRRMRHVRGWPLRIGLAVAAILAFSGLHIAGMVGLRKLGMFLVGGAYDFHFSAATLIY
                     EFRKDVITCLLIGGALWLIGSHREAQQARSVVATARADPPSVAPHMVWLRDGSTRIRI
                     EPRDILWISSAGNYVEYSLADGVNHLVRGTLAAEEARLTPFNIVRVHRTRLVNLTRVR
                     GLKTGANGDFELTLDTGQTISGSRRYRGAVASIEELAANPASVLTNNGAHS"
     gene            8512..9159
                     /locus_tag="VN968_20425"
     CDS             8512..9159
                     /locus_tag="VN968_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL31636.1"
                     /translation="MPEVIFTGPAGRLEGRYHPAKQKNAPIAMVLHPHPQFHGTMNHQ
                     IIYQCYYAFAHRGFSVLRFNFRGVGRSQGSFDHGTGELSDAASALDWAQTINPEARAC
                     WVAGFSFGAWIGMQLLMRRPEVEGFISIAPPANLYDFSFLAPCPSSGLIVHGEKDAVV
                     PPKDVNTLVEKLKTQKGIVIDQQVIPGANHFFDGKLQPLMETVTGYLDMRLANVR"
     gene            9175..9687
                     /locus_tag="VN968_20430"
     CDS             9175..9687
                     /locus_tag="VN968_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HXL31637.1"
                     /translation="MSSFVALLRAVNVGGTGKLPMTDLKEICEDLGFGAVRTYIASGN
                     VVFSSRKSEAAIKAALEKRLEAYARKPVGVLVRTAAEMAQVLADNPFPKAAPNRTMAV
                     FLDRAPPPDTLDGIRGQRDEQVQLGRREIYIHYGEGMAASKLVIAAAKTGTARNMNTV
                     ATLARMAAES"
BASE COUNT         1894 a         3235 c         3004 g         1852 t
ORIGIN      
        1 tgggcggcga tccgttcctt gccgatcgaa ttgacgtagt cgatcgccgc ccccagtccg
       61 atcgattcca cgatcgcggg cgtgcccgcc tcgaacttgt gcggcgggtc gccataggtg
      121 acccagtccc tcgcgacctc gcggatcatc tcgccgccgc cattataggg ccgcatcgcc
      181 accaggtgtt cgtgcttggc gtagagcacg ccgatgccgg tcgggccata gagcttgtgg
      241 ccggtgaaga cgtagaaatc gcaatcgatg tcctgcacgt cgatggcgag gtgcaccgcg
      301 gcctggctgc cgtcgatcag caccgggatt ccccgcgcat gggcaagttt tacaacgtcc
      361 ttgaccggca cgatggtgcc gagcgcattc gacatctggg tgatcgcgac cagtttggtg
      421 cgcggcgtca gcgccttttc gaactcgtcg atcaggaaat tgccctcgtc atcgacgtcg
      481 acccacttga tgacggcgcc gtgacgttcc ctgaggaaat gccaaggcac gatgttggaa
      541 tggtgctcca tgatcgagag cacgatctcg tcgccagcct tgatattcgg ttcgccccat
      601 gacgacgcca cgaggttgat cgcctcggtg gcgttgcggg tgaacacgat ctcttcgtta
      661 cgcccggcgt tgaggaattt tgcaactttg gcgcggccgc cctcataggc ttccgtcgcc
      721 gcattggcga ggtaatgcag cccgcgatgc acgttggcat attcgctctt gtaggcttcc
      781 gtcatgcggt cgagcacggc attgggcttt tgcgccgacg ccgcgttgtc tagatagacc
      841 agcggcttgc catagacctt taacgtcagc gccggaaagt cttcccgcac ccgggctaca
      901 tcatatgaac cgttcaggac tgccggatgc atggtcaacc tcttgccgcc agccagcgct
      961 gcgccgccga gatcgcgagt tcgcgcaagc catcatgggc gatggattcg atggcctccc
     1021 cgacgaaggc ctggatcagc agtgcctgcg cttccttctc ggacaggccg cgcgcgcgga
     1081 ggtaaaacaa aaggctctcg tcgagcgcgc cggtggtagc gccgtggccg caggtgacat
     1141 cgtcggcgaa aatttccagt tccggcttgt tgtcggcctc ggcctcgtcg gacagcagca
     1201 gcgcccgcgt catcatcttg gcatcggtct tctgcgcctt gggacggacg atgatgcggc
     1261 cctgaaacac cgaatggccg cggtcgtcga ccacggcgcg gaagagctcg cggctggcgc
     1321 agttcggcac cgcgtgatcg agaaacagcg tggtgtcggc atgctggcgg ccattgagca
     1381 gattgacgcc gttggtctcg acccttgatc cctcgccggc aaacgcgatc accgcatgat
     1441 agcggctgac actcgagccg ctggtcatgc caaacgtgtt gaagtgcgca tgcgcgccca
     1501 atcgcaacac ggccgaggag atattgaagg cctcgcggcc atcttcgatc aggcgcacat
     1561 gatcaagccg cgcgccatcg ccgatcgcaa ccaccagcga gtcgtgaacc tgataggcct
     1621 tcgcgccatc ggcagcgata tagctttcca ccagcgtcgc acttgcggcc ttgccgacct
     1681 tcaacaccga gcgcgtgaac atcgccgcgg gcgcagcacc gctggcgata tggacgatat
     1741 gcaggggctg tgtgagcacg gcgccatcgg caacctcgat cacgaggcca tccgtcatca
     1801 tcgcgctatt gagcgccacc gtggaatcgg agttttcggg cgcgaacagt agcgcatgca
     1861 gtgcggcatc gccggcttcc agcacgtcgc gcagcgtgcg gatgctgagg ccttttccaa
     1921 gaccagccac ctcggacagt tttggcgcaa atacgccgtc gaccagcacc agccgacgcg
     1981 cgcccccgat ggcgtgaagc ttcaaggccg tgcccgcgcg cgtcagcgcc gccgcgtccg
     2041 gcgccggcgc cagcggcagc acctcgcgca tcagcaggcg cagatcggtg tatttccagt
     2101 cctcgatgcg ccggtgcggg aggccaacac gctcgtaagc ctcgaatgcc tgccgcctgt
     2161 gatcagcgac cttgcctgcg cccggcagcc gatcctgagc gatggcgaag atatcgctca
     2221 gcgcgtgtcc tttttcggtt ttggccagta ctacgttcat tacaaaatcc gcaattaggc
     2281 gtcaggccgc gtcttcgaac tgggcatagc cggaagcctc gagctccagc gccagttcct
     2341 tgtcgccgct cttcaccacg cgccccttcg acatcacatg cacgaaatca ggcacgatgt
     2401 aattcaacag ccgctggtaa tgcgtgatca ccaccatggc gcggtcaggc gaacgcagcg
     2461 cattgacgcc gtcggcggca acccgcagcg cgtcgatgtc gaggccggaa tccatctcgt
     2521 cgaggatgca caggctcggc tcgaacaaag ccatctgcag aatctcgttg cgcttctttt
     2581 cgccaccgga gaacccgaca ttgacgccac ggcgcagcat gtcctgcgga atgttaaggg
     2641 aggcggcgac gctacgcact ttcttcaaga agtccggcgt ggagaattcg ctctcgccgc
     2701 gcgccttgcg ctgcgcgttc agcgcggtgc gcaggaacgt catggtggcc acgccgggaa
     2761 tttccaccgg atactggaac gccaggaaca cgcccctggc ggcgcgttcg tcgggtgcca
     2821 tggcaagcag gtcttcgccc ttgaacagga tttcgcccgc cgtgacttcg tagcccggct
     2881 tgcccgcaat gacgtgggac agcgtcgatt tgccggagcc gttcggcccc atgatggcgt
     2941 gcacctcacc caggttcaca gtgagcgaaa gcccgtggag aatctcacgc tcctcgacac
     3001 ggacctgcag atttttgact tcgagcaacg acatatcttc aaactttcta ttcgcgcgcc
     3061 gacgctcttg cgccaacgcc attcgccatc cgtttttgcc attcgccagc gcgaggaacg
     3121 gtcaacgccc agccttgcca ggtcgtcgga acgttgccgt agccgcgctc cctgggtttg
     3181 aaccaatacg cgctcatcac ccaacgctgc cttccagcga gatcgagatc agtttctgcg
     3241 cctcgaccgc gaattccatc ggcagttgct gcagcacgtc cttcacaaac ccgttgacca
     3301 cgaggccgac ggcctcttcc tgcgacagcc cgcgctgcac gcaatagaac agcacgtcct
     3361 cggagatctt cgacgtagtc gcctcgtgct cgaacagggc cgaagagttt ttcgcctcga
     3421 tatagggcac cgtgtgcgcg ccgcatttgt cgccgatcag cagtgagtcg caggccgtaa
     3481 aattgcgcgc gccggtcgcc ttgcggtggg cggtgacgag gccgcgatag gtgttctgcg
     3541 agatgccggc ggcaatgccc ttggagatga tccggctggt ggtgttcttg cccaagtgaa
     3601 tcatcttggt gccggaatcg acctgctggt ggccgttcga aatcgcgatc gaatagaact
     3661 cgccgcgcga attgtcgcca cgcaggatgc agctcggata tttccaggtg atcgcagatc
     3721 cggtttcgac ctgggtccag gaaatcttcg aattgttgcc gcggcaatcg ccccgcttgg
     3781 tgacgaaatt gtaaatgccg cccagccctt ccgagttgcc gggataccag ttctggaccg
     3841 tcgaatattt gatctcggca tcgtcgagcg tgacgagttc aacgacggcg gcgtgcaact
     3901 ggttttcgtc gcgctgcggc gcggtgcagc cttcgaggta gctgacataa gcgcccttgt
     3961 cggcgatgat cagcgtccgc tcgaactggc cggtgttgcg ctcgttgatg cgaaagtagg
     4021 tggacagctc catcgggcag cgcacgcccg gcggcacata gacgaacgag ccgtcggaga
     4081 acaccgccga gttcaacgtg gcaaaataat tgtccgaggt cggcacgacc gtgccgagat
     4141 acttcttcac gaggtccgga tgctcgcgga tcgcctccga gatcggcatg aagatcacgc
     4201 cggcctgttt taactctttc tggaacgtgg ttgccacgga aacagagtcg aacacggcat
     4261 cgaccgcaat gcgccgctcg ccctcggggc gcaccacgcc ttccagcacc tcgacctcgc
     4321 gcaggggaat gccgagcttt tcgtaggttt tgaggatttc cggatcgacc tcgtcgagcg
     4381 agccgatcgt cttcttcggc ttcggcgccg aatagtaata gagctcctgg taatcgatct
     4441 tgggatagtg gacgcgggcc catttcggct cggtcatggt cagccagcgg cgataggctt
     4501 ccagccgcca ctccagcatc caggccggtt cgttcttttt cgctgagatg aaacggacgg
     4561 tatcttccga gagccccttc ggggccttgt cggattcgat aagcgtctca aatccatatc
     4621 gatattggtc gacgtcgatc cggcgcaccc gatcgacggt ctcttgtacg gccgccattc
     4681 cattcctccg ctcgcggttt caaggaccgc ggtggatcaa ttccgaaata ctattacgat
     4741 gttccgccgg cgaaatcacc tgccttagaa ccgttcaagc tgtgtttcgt cgcaactcct
     4801 taagtagggt atcggtgagc tttcgccaag cctcaaggca gcgatcaatg tctgcctctg
     4861 acgtggacca gcccagactg agccgcaccg ctccttgagc ggtattcgcg ccgtatccca
     4921 tggctttcag gacgtgggag ggttgtacct ttcccgacga acaggcggaa cccgaggaaa
     4981 ccgcgatacc ttcgaggtcg aaaccgataa cggcggtctc cgccttcagc ccgggaaccg
     5041 taaaaagcgt ggtattcggc agccgcggca cgtcgccgga gaacacaatt gcccctgttg
     5101 tctcccgcaa gcccctctcg agccgattcc ttaagctttc gaggcgaatg gcatcgcttt
     5161 cgcggacgcc catggcggcc ttcgccgccg cgccaaaccc cacaattccc gcaacattct
     5221 cggttccggc ccgatgcccc agttcctggc cgcccccgcg cagccacggt tcaaggccgc
     5281 ggaccccctc ggccagagcc acggcgccca cgcccttcgg cccgccaatc ttatgtccgg
     5341 aaaccgtcac cagatcggca ttcaacgctt tgatatcaaa ataaattttt ccgagtgcct
     5401 ggatcgcgtc aacatgcatg agcccgccgg cggcgtggac gatctcggca gcctctgccg
     5461 ccggttgcac ggcgccggtt tcgttgttgg caagcatcac cgacaccagc gcgggtggcc
     5521 cggcctcaac ctttgtgcgt aggcggtcca gatccagcac gccggcgcgg gtgacgccga
     5581 tcgtgcccat cgcttctgtt gggaaccgcc ctcccgccag caccgaggtg tgctcgatgg
     5641 ccgagaccag tagccgctcg accggaagcc ctgagccgcg ccgcagccca ggagtcagcg
     5701 ccagtgcatt cgcctcagtt ccacccgacg taaagacgac gttgcgaggc agggtgccga
     5761 ccgcaccggc gaccgccgcc ctcgcctcct cgaccagtct gcgggcctgg cggccctcgg
     5821 catggaccga ggacgggttg ccgcagaaat cccacgccgc ggccatcgcc gccctggctt
     5881 gggggcgaag cgggctggtc gcattccagt cgagatagac gcgatcgggc atatttcgtc
     5941 ttagcacttt tcctcgaggg acaaatcatt gcgccggttg ggctgtcggc cgcaccggag
     6001 tctgctcgaa tggggacacg cgcaacccga acagcggcct taagatggct attcgcctga
     6061 caatctcata ggtcagcacg cagaccgcca acgtgcccga tatcacgatg ctcgcttcca
     6121 gccaggccgg aagatcgcgg ccgtgcagtt cgtgagcgat catgatgatc gcggtctgat
     6181 ggacgatgta atacggaaag atcgcatcgg taagatatcg gcgcacgcct gagtccgccg
     6241 tgagccagcg cctggcgaaa ccaagcacgg cgaccatgca gaaccattga tagcagccgt
     6301 aagcgatccc gccatagagc ttcagcagca gcgacggcgg cgccccggac tctctggtcc
     6361 accgcaacac cagaaaggac agaaagaccg ccgccgcgag cgacagggcc aaccggcgct
     6421 ggcgctcgat cgcgtcccaa aaagcctccg cgcgcgccag cagaaagcca agcaggaaga
     6481 cgctggcgta aaccgcgtga ttgtaccagt cgccgaacag cgcatgcgtg gacggaaagc
     6541 tcggcagcac ggcgaggcgg taggcggcga acgcaaccga tggcgcgacc agcagccccg
     6601 cgcccgataa cacgggcacg agcctccgtt ccacccagcc tgtaagcccg ggaaacgcga
     6661 cccaaacgac gccgagcgcc atggtgtaaa tccacaaata ggccacgaac cacagatggt
     6721 tccatgtcgg cagcagaatg cagggaccgg gaccgcagaa ctgcggcccg aaggcaaaat
     6781 agtggcgcgc atagaaatca gggaaaccgg cgggatagcc caggctttcg acaatctggt
     6841 cgtaggcctg cggcggcacg atcacgagca tgccgaaaat cagcggaatc aacagccgga
     6901 ccgatctcga gcgaagcagc ggaccaacag cgtatttgcc caacatgaat cgggttgcgg
     6961 cgcccgagac cagaaacagc agcgccagcc gccacggatt gagcaccagc atcagcggct
     7021 ccagcgccgt gatgcggtgc gcgctcttga tgtggaaccc ccaggacacg tagagcatgc
     7081 cgacgtggta aaggatcagc agcccgaagg cgccgatccg cacccagtcg agatcgacgc
     7141 ggcgcgccga cctttcggga gccaaatgcg gtgaatgtga tacggtttgt cgcttcatgg
     7201 ttcgatcccc gaatgcgcga tgggcttcat gagtgacgtc aaaaatgcgc aaggcggccc
     7261 ccgcgtcgag ccggtttggg accagaaacc acctgcctgg gatgaagcga gaccgcgtgg
     7321 gacaagcggc ggccttggcg ggatatccgg tgccgattgg ctgctctatg gcgcggtggc
     7381 agcgatctcg ctggggatcg gggtagtcaa tgccctctcc aaagcgcagg acgtcgcctg
     7441 gcgcggcggc gtctatgatg cccgcacgca gctgttctgg gagctgtcga gcatcgtcac
     7501 gatcatcctg gttgcgccgg tgctgttcgt cgcggtgcgg cggatgcgcc acgtccgggg
     7561 atggccgctc cgcattggcc ttgcggtggc ggcgatactc gctttctcgg gcctgcacat
     7621 cgccgggatg gtcggccttc gcaagctcgg gatgttcctg gtcggcggtg cttacgattt
     7681 tcacttctcg gccgcgaccc tgatttatga gttccgcaag gacgtcatta cctgcctgtt
     7741 gatcggcggc gcactatggc tgatcggcag ccaccgcgaa gcgcagcagg cccggtcggt
     7801 cgtggcgaca gcgcgggcgg acccgccgtc tgtggcgccc cacatggtct ggctgcgcga
     7861 cggttcaacg cgcatccgca tcgagccgcg cgacattctc tggatcagct cggccggaaa
     7921 ttacgtcgag tacagtcttg ccgacggcgt caatcacctc gtccggggca ccctggccgc
     7981 cgaggaagcg cgcctgacgc cgttcaatat cgtgcgggtg caccggacac ggctggtcaa
     8041 tctcacgcgc gtgcgcggac tcaagacagg tgcgaacggc gatttcgaac taacgctcga
     8101 caccggccag accatctcgg gcagccgccg ttaccgcggc gcagtcgcct cgattgaaga
     8161 gcttgccgcc aatcccgcct cggttctgac caataacggc gcgcattcct gatgcggtct
     8221 gccgggaaac ctcggccggc cgccgccagc cgctaatttc tttcggcatt acaaaacctt
     8281 attgcgagat tttaatcgct aaactcggcc gtccggatgc acaaaaccct tgctttttgc
     8341 ccctcgccct atgctagaac gcggcctcca gttcaccgag cgcccgtccg cttaagccca
     8401 acggcgcctg atccgcccct ttatctctgc cgagattcat ctcgcaaaac agccagatgg
     8461 agccggcaca aaggcggttg gttttcgccg agggatcatc aagggatcac gatgcctgaa
     8521 gtcattttca ccggccccgc aggccgcctc gaaggccgtt atcatccggc caagcagaag
     8581 aacgcgccga tcgcaatggt gctgcacccg catccgcagt ttcacggcac gatgaaccat
     8641 cagatcatct accagtgcta ctacgcgttc gcgcatcgcg gattttcggt gctgcgcttc
     8701 aattttcgcg gcgtcggccg cagccagggc tcgttcgacc acggcaccgg cgaactttcc
     8761 gatgccgcct ccgcgctgga ctgggcgcag accatcaatc ccgaagcgcg cgcctgctgg
     8821 gtcgccggct tctcgttcgg cgcctggatc ggcatgcagc tgttgatgcg ccgtcccgag
     8881 gtcgaaggtt ttatttcgat cgcaccgccc gccaatctct atgacttctc gttcctcgcg
     8941 ccctgcccgt cctcgggact gatcgtgcat ggcgagaagg atgccgtggt gccgcccaag
     9001 gacgtcaaca cgctggtcga gaaactgaaa acccagaagg gcatcgtgat cgaccagcag
     9061 gtcatcccgg gcgcgaacca tttcttcgac ggcaagctgc agccgctgat ggagaccgtg
     9121 accggctatc tcgacatgcg gcttgccaac gttcgctgag cggtcgggtt ggccatgagc
     9181 tcgttcgtgg cgctgctgcg tgcggtcaat gtcggcggca ccggaaagct gccgatgacc
     9241 gatctcaagg agatctgcga ggatctcggc ttcggagctg tccgcaccta catcgccagc
     9301 gggaatgtcg tcttcagcag ccgcaagtcc gaagctgcca tcaaggctgc cctcgaaaag
     9361 cgactggaag cctacgcccg caagccggtc ggtgtgctgg tgcgaaccgc cgccgagatg
     9421 gcgcaggtat tggccgacaa tccatttccg aaagcagcgc ccaaccgcac catggccgtg
     9481 ttcctcgacc gcgcgccgcc cccggacacg ctggacggca tccgcggcca gcgggacgaa
     9541 caagtccagt tggggcgccg cgagatctac atccattacg gcgagggcat ggccgcgtcc
     9601 aagcttgtca tcgccgccgc caaaaccgga acggcgcgca atatgaatac cgttgcaacg
     9661 ctcgcccgga tggccgccga gtcctgacgc agcgatcgtg cgattacgcc gccacccgcg
     9721 cctgcaggcc tgacagccag gaattccgac ggtcgatgaa gccacgaagc accgccggat
     9781 agtcaccgct gacggccgtg cgcaccgacg gccgcgctgc cagggcctcg cgccaacgct
     9841 ttagcccaac ttgcgcagaa tcgagggtcg ggttcagaca aatcaatgcc ttagttgaaa
     9901 agttggcact acattttctg caaagttgct gtgggccgcg gttattcccg cgcgaatcac
     9961 atctcatgaa cgagaatcat gctcg
//
LOCUS       DATNOI010000509         2037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1641412,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000509 DATNOI010000000
VERSION     DATNOI010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641412"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VN968_20435"
     CDS             <1..364
                     /locus_tag="VN968_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045861.1"
                     /note="catalyzes the reaction of formaldehyde with
                     glutathione to form S-hydroxymethylglutathione; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde-activating
                     protein"
                     /protein_id="HXL31638.1"
                     /translation="PRDSVTVLENGDKLHVIDASAAIQRHACKVCGTHMYGRIENKAH
                     PFYGLDFIHPELFQEAGSAAPGFAAFVSSVIESGVKPDEMPGIRSRLKELGLEPYDCL
                     SPALMDAIATHVAKSKAA"
     gene            631..1710
                     /locus_tag="VN968_20440"
     CDS             631..1710
                     /locus_tag="VN968_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL31639.1"
                     /translation="MPQDSSLEVAGRIDDWWSLAKHDISTDPFGRMARAGLCQIGLPG
                     AGAALDTYRAIAAAEQAIAAKTGLLGLASAFAARQMTARFFIAGFADADQRATWLPRV
                     AAGEVCAAIAISEPEVGAHPKHLKTTAEPDGAGFVVRGWKAWVTNGPIADVFLVLAVT
                     AVEDGRKRYGLFLIPRETPGLTVKPMQTLEALAPASHCELEFDACHVPATARVGDMLD
                     AYPAMALPFRDVEDTVGTANVSGMLSWLLEKSAACIERTEANALRLGRIAGLVSLVHA
                     ASRLAVAALDGDGQEVAARTIGVRLLAQDIVGEIRHLLAQVQPGDDATSRALAAFDLL
                     SSIARQPRKVRQIRLGNSAWSEKQQ"
     gene            1707..>2037
                     /locus_tag="VN968_20445"
     CDS             1707..>2037
                     /locus_tag="VN968_20445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXL31640.1"
                     /translation="MNNNLTADSIEAVTSGLASAGYIASRQIATAVYLAERIEKPILV
                     EGPAGVGKTELAKALAAWRGLKMIRMQCYEGLDEAKALYEWKYAKQLLYTQILKDKLG
                     EVLGGAET"
BASE COUNT          376 a          628 c          672 g          361 t
ORIGIN      
        1 gccacgcgac agcgtgaccg tgctcgagaa cggcgacaag ctgcacgtca tcgacgcctc
       61 ggcggcgatc cagcggcatg cctgcaaggt ctgcggcacg cacatgtacg gccgcatcga
      121 gaacaaggcg cacccgttct acgggctcga cttcatccat cccgaactgt tccaggaagc
      181 cggctcggcg gcgccgggat tcgcggcgtt cgtgtcgtcc gtgatcgagt ccggcgtcaa
      241 gccggacgaa atgcccggca tccgctcgcg tctcaaggag ctcgggctcg agccctatga
      301 ttgcctgtcg ccggccctga tggatgcgat cgccacccat gtggcgaaat ccaaagcggc
      361 ctgatccggc agccttgcgt aagcttttcg ctggcggcga tctccgccgc cagcgaagtt
      421 gcgaccactt ttgacacgcg gcaatctggt gccgcgttgg agtcatgccg gcttgtcccg
      481 caaggcggaa ttgcacagtc aacactgctg aaaacgtgcc cttcgcgacc gcggtcgggg
      541 gaaatttgag ctcgcgacca acacccgtgt gccttaaaag cgtcttgatg ccgcaagagg
      601 cgcgcgtcgg gaatccgggt cgttcatcag atgccgcaag attcgagcct ggaagtcgcc
      661 ggacgcattg atgattggtg gtcgctcgca aagcatgaca tctcaaccga cccgttcgga
      721 cggatggcga gggcaggtct ctgccaaatc ggcctccctg gcgcaggagc ggcactcgat
      781 acctatcgtg ccatagcggc cgccgagcag gcgattgccg caaagaccgg actgttgggg
      841 ctggcgagcg cctttgccgc ccgacagatg acggcacgat tctttattgc gggttttgcc
      901 gacgcggacc agcgtgccac atggcttccg cgcgttgcgg ctggcgaggt ctgcgcagcg
      961 atcgcgattt ccgagcctga agtcggagcc catccgaagc atctaaagac gacagccgaa
     1021 ccggacggtg ccggcttcgt cgttcgcggc tggaaggcct gggtcacgaa tggcccgatc
     1081 gcggatgtct ttctggtgct ggcggtgacc gcggtcgagg acggacgcaa gcgatacggg
     1141 ctttttctga tcccgcggga gacgcccggc ctgacggtaa agccgatgca aaccctcgaa
     1201 gctctcgccc cggcgtcgca ctgcgagctg gagttcgacg catgtcacgt gcctgcgacg
     1261 gcgcgggtgg gagacatgct tgacgcctat ccggccatgg ccttgccgtt tcgcgatgtc
     1321 gaggacaccg tcgggacggc taacgtctcg gggatgctca gctggctgct cgaaaagtcc
     1381 gccgcgtgca tcgagcgcac ggaagcgaat gccttgcggc tcggccgcat cgcgggtctg
     1441 gtatcgctcg tgcacgcggc gagccggctc gcggtggcgg ccctcgatgg cgacgggcag
     1501 gaagttgcgg cgcggacgat cggcgtgcgg ctgctggcgc aggacatcgt cggagaaatc
     1561 cgtcacctgc tcgcacaggt tcagccgggt gacgatgcga cctcccgggc gctcgccgcc
     1621 ttcgatctat tgtcctcgat cgcacgccag ccccgcaagg tgcggcagat ccggcttggc
     1681 aattcggcat ggagcgagaa acaacaatga ataacaatct aaccgcggat tcgatcgagg
     1741 ctgtcacatc gggactggcc tcggcgggct atattgccag ccggcaaatc gcgaccgcgg
     1801 tctatctggc agagcgtatc gagaagccga tcctggtcga aggtccggcc ggcgtcggca
     1861 agaccgaact tgccaaggcg ctcgcggcat ggcgtgggct caagatgatc cgcatgcaat
     1921 gctatgaggg gctcgatgaa gccaaggcgc tgtacgaatg gaagtatgcc aaacagcttc
     1981 tctataccca gattctgaag gacaagctcg gggaagtgct cggcggcgct gaaacgc
//
LOCUS       DATNOI010000510        12771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_206555,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000510 DATNOI010000000
VERSION     DATNOI010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12771
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206555"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..90
                     /locus_tag="VN968_20450"
     CDS             <1..90
                     /locus_tag="VN968_20450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HXL31641.1"
                     /translation="LAAKFETPVNTVKTWLRRSMLDIRECLGL"
     gene            94..984
                     /locus_tag="VN968_20455"
     CDS             94..984
                     /locus_tag="VN968_20455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HXL31642.1"
                     /translation="MADSEDDIALAAEYALGTLDADERAQVETMMSRDKDFAAIVEAW
                     EYKLGILNQMVGSIEPPAEVWDKIKTAVGHAEPQSPLLLPDAAPPPPPAAAEVVEPAA
                     APVDTSSVIRLSAQARRWRRVATFTTAIAAALVAMIAVGVYQPDLLPDGIRPKPRVQI
                     VEVKPPAVPAPSAQYVAVLQKDGGSPAFILTVDAATKNFTVRKVGAAAEPGKSFELWL
                     ISDKLPRPRSLGVIGGADFTARPVLSSYDTDTINTATYAVTVEQAGGSPDGNPHSAPV
                     FTGKLIESVPSAAPAPSPAR"
     gene            complement(1192..1392)
                     /locus_tag="VN968_20460"
     CDS             complement(1192..1392)
                     /locus_tag="VN968_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31643.1"
                     /translation="MNHSIYSADRTTHLKIVVVALVAGIAVAGFGISARNNSDDGYTQ
                     TARVIKAGKAVTVTSANTSAVR"
     gene            complement(1857..4088)
                     /gene="flhA"
                     /locus_tag="VN968_20465"
     CDS             complement(1857..4088)
                     /gene="flhA"
                     /locus_tag="VN968_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HXL31644.1"
                     /translation="MDASAVPHFRNGGPSAAAKTFGARDRHMRGEAATMVDITAGQGG
                     ATGGGFPSLSEIGTILKRGDLALAFGILTILVVLILPLPSIVLDLFLAISITLSILIL
                     MTSLFIQAPLEFSSFPTILLISTMLRLSLNLASTRLILSRGHEGTAAAGHVIEAFGNF
                     VMGGNFVIGIIVFAILVIVNFVVITKGSGRIAEVAARFHLDAMPGKQMAIDADLSAGL
                     IDEKVAKERRKALEDESGFFGAMDGASKFVRGDAVAGLLVVFINVIGGIIIGVAQQGL
                     GFGEAARTYTLLTVGDGLVTQVPALIVSTAAGLLVSKAGVSGAADKALMKQLSGYPQA
                     LGMSAGVMLVLGMLPGIPMIPFFLLGSGAAALAFSARKRNRATKQAEAVAAAAPAAAA
                     AAASAAEEPISTALKIDDLKIELGYALLPLVNGPDGTDRLTEQIKALRRSLAVEMGFV
                     MPAVRILDNVQLEANTYIIKIKEVDAGTGKIWPSQFMVMDPAGNQVGVPGIHTIEPTF
                     GLPATWVDASLKEEATLKGYTVVDAATVLSTHLTELLKNNMSDLLSYGEVQKLLKDLP
                     KEQGELVKDIVPSMVTISGIQRVLQLLLAERISIRDLSTILEGIADALAFSRNPAAVV
                     EHVRARLARQICAQNTSMNGYLPLIALSAKWEQAFAESLVGQGEERSLAMQPSRLSEF
                     MTAVRDRFEQAAREGESPVLVTSAAIRPFVRSLVERFRSQTTVLSQAEIHPRARLKTV
                     GSV"
     gene            4382..5617
                     /locus_tag="VN968_20470"
     CDS             4382..5617
                     /locus_tag="VN968_20470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL31645.1"
                     /translation="MHAPSDSSKDSAAFTPDSGQAWVRLGLALIIGSIGSVGMWSVVV
                     VLPGVQAEFGATRGAVSLAFTLVMLGFGLGGVATGRITDRFGIVLAVALSICFLGIAY
                     VLAGLSTTLWQFIAVHFLIGLGTSATFAPLMAEVSHWFERYRGLAVGIVASGNYVGGT
                     IWPPLVNWGTQVTGWRTTHIAIGIFCVVMMTLVLLVLRARIGGATLRDHENAPPPQVD
                     LRLSTNTLTTLLCVASISCCVAMAMPQVHIVAYCGDLGYGVARGAEMLSLMMAFGVVS
                     RIGSGFLADRIGGIRTLLIGSVAQGFALLFYLFFDGLTSLYVISAMFGLFQGGIVPSY
                     AIIVRESMPAREAATRVGLVILASVFGMSFGGWVSGVIFDATGSYAAAFLNGLGWNAL
                     NIAIMVSLLIRVRQRLALA"
     gene            complement(5700..6077)
                     /locus_tag="VN968_20475"
     CDS             complement(5700..6077)
                     /locus_tag="VN968_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXL31646.1"
                     /translation="MKTVQAMLAEAEAVVPRISPEDAKGLLGRTDVLFLDVREPAEVA
                     ASGKVPGALAIPRGLVEFRADPASPLHDAAFDQAKTVVAYCASGGRSALVGKTLKEMG
                     YANVQNLGGFKGWLDAGGEVEKA"
     gene            complement(6243..6722)
                     /locus_tag="VN968_20480"
     CDS             complement(6243..6722)
                     /locus_tag="VN968_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase enzyme"
                     /protein_id="HXL31647.1"
                     /translation="MTIITVTTPEKRLSLAQRGRLAETLTDAVLEPEVGQFAPAARAG
                     FQVHFRELPADCMAIGGKLLAELDSPRDIMTIHIAVMNAAWPTEVRRRVIRGVLARMA
                     EAFNLPKPSPTWWVTFQIIDEGSWGSRGDVLSILQLLESGAFSEARISAIRAAIASS"
     gene            6778..7443
                     /locus_tag="VN968_20485"
     CDS             6778..7443
                     /locus_tag="VN968_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL31648.1"
                     /translation="MSIVIIYGWPEKTWGRLAMNSDTRAKMVAGAADLLSRRGVNATS
                     VREVVRHSGTPRGSIGHHFPRGKQQLIEDAIAFAGAEVSAPLEHLMRERGAIAGLRAF
                     IALWRRTLEASHFEAGCPVLAVAVEQYVSDAMDKGGARDESVQQHLRDLAHGVFADWQ
                     RIIATALLREGAGAARARRLAALVVASVEGTVAMCRAARSARPLEDIRKELELILSAS
                     IPK"
     gene            complement(7476..7884)
                     /locus_tag="VN968_20490"
                     /pseudo
     CDS             complement(7476..7884)
                     /locus_tag="VN968_20490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030249.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
     gene            complement(8033..8917)
                     /locus_tag="VN968_20495"
     CDS             complement(8033..8917)
                     /locus_tag="VN968_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXL31649.1"
                     /translation="MRKVWIEAALNGAWSRALQPGIPDTIEAIIADGVACARAGATII
                     HTHAYDGGGLQTFDWQVYARIIEGIRAQVDVPVYPSYPAFMTTAADTGPSFADATVRF
                     AHIEALAARGLLDFAIIDPGSVNFSRTKTTPAASPAATYMNPESHVRHALEFAARHGF
                     HPAFAIYEPGFTRAGAALARAAGVKTPIYRFMFCETIAVGFPPRPYALAAHLALLEEE
                     AGLAPWMIAGVCADPRPLIGETVARGGHIRVGLEDAPLRTPLSNLALVEEAVRIVRDH
                     GAEPASVADMREALGQLP"
     gene            complement(8887..9588)
                     /locus_tag="VN968_20500"
     CDS             complement(8887..9588)
                     /locus_tag="VN968_20500"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL31650.1"
                     /translation="MPRPVRRTTPKDSGKNTGTYSRENGARARIVAAALEELVAGDGD
                     FEIGDVARRAKVSVGLAYHYFGSKAGLISVLITEFHNRHEAVFNQSFDESLPWPVREK
                     ARLKASIDFMYADPVAPVMMGKLSSSAEVIAIDTGRRSAVVEMAARNVAQGQRRGFIA
                     ADIDPDIAGAVIIGGVNQAVARAISSRRRPDPDQLADRLWSFIAGGLSLAPQGVPNEK
                     QKEIPRCARSGSKRR"
     gene            9671..10540
                     /locus_tag="VN968_20505"
     CDS             9671..10540
                     /locus_tag="VN968_20505"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXL31651.1"
                     /translation="MPTTIEQASPWLQGRTFASWREEFDHRGYLFFERVLSPDRVTAI
                     RTALAPYLERDLTGRNDFEGLKTNRVYALMAKSPLFAELAIHPLALAFAEAELGQSCL
                     LSALLAINLHPGETVQPWHFDDGGVKLPRPRPAFGVSAFWAIDHTTESNGSTEVIPGS
                     HLWDGRIVEGAVKPADFANTAAPNPDSDFSNRPDAVKLVMPSGSLAITKGTLWHRGGA
                     NLSDRPRLIITPQYCAGWVRQLENMALAVPAALAEKLPERARELIGYSIHPPFMGHVD
                     GVHPKRLLRSRVT"
     gene            complement(10578..10871)
                     /locus_tag="VN968_20510"
     CDS             complement(10578..10871)
                     /locus_tag="VN968_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31652.1"
                     /translation="MKLTSAQVERTINQFEAQALPDNHPAVPQLNELFGDHTFLLDIN
                     GLNILEPADQSPRDRVQAARVVNLASWSDEALTSLAPHEPEPTGAIIELESKH"
     gene            complement(11224..11481)
                     /locus_tag="VN968_20515"
     CDS             complement(11224..11481)
                     /locus_tag="VN968_20515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31653.1"
                     /translation="MTETAMKRSLICCCLLMILGSPGHSQIAKATPVFYIVLNSLTKN
                     CRVVDRMPRTNTPDITVASDTIYKTRAEAETAVQTLTPCKQ"
     gene            complement(11779..11988)
                     /locus_tag="VN968_20520"
     CDS             complement(11779..11988)
                     /locus_tag="VN968_20520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31654.1"
                     /translation="MLHLSKLVEYLAPGFVGMASDHVVETARSGRGHNVRRFTLGEEH
                     DATGHEALTLLDCKVTAQVEVNSSC"
     gene            12190..12333
                     /locus_tag="VN968_20525"
     CDS             12190..12333
                     /locus_tag="VN968_20525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31655.1"
                     /translation="MKAPGLIVQATKLQERAREPIGYSIHPPFMGHVDGVRPGRLLQP
                     QAA"
     gene            12569..>12771
                     /locus_tag="VN968_20530"
     CDS             12569..>12771
                     /locus_tag="VN968_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020572214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL31656.1"
                     /translation="MPIRCLLATSLILSCLSVAPMKAAETVTSAVPGSRTTTYKTVAV
                     NGLNIFYREAGPSNAPAILLLHG"
BASE COUNT         2354 a         3925 c         3988 g         2504 t
ORIGIN      
        1 ctggcggcga agttcgagac accggtgaat accgtgaaga cctggctgcg ccgcagcatg
       61 ttggatatcc gggagtgtct tggactgtag atgatggccg acagcgaaga cgatatcgcg
      121 ctcgccgcgg aatatgcact cggcaccctc gatgccgacg agcgggcgca ggtcgagacc
      181 atgatgtccc gcgacaagga cttcgcggcg atcgtcgagg cgtgggagta caagcttggt
      241 atcttgaacc agatggtcgg atcgatcgag ccgccggccg aggtctggga caagatcaag
      301 accgcggtag gacacgccga accgcagtcg ccgctgctgc ttcccgacgc cgcgccaccg
      361 ccaccaccgg ctgccgctga agtcgttgag cctgccgccg cgccggtgga cacctccagc
      421 gtcatccggt tgtccgccca ggctcggcgc tggcgccgcg ttgcgacgtt caccacagcg
      481 atcgcggcgg ccctggtcgc gatgatcgcg gttggggtct atcagccgga tttgctgccc
      541 gatggcattc gtccgaaacc gcgcgtgcag atagtcgagg tcaagcctcc tgcggtgccg
      601 gcgccgtccg cgcaatatgt cgcggtgttg cagaaagacg gcgggtcgcc cgcgttcatt
      661 ctcacggttg atgcggcgac caagaatttc acggtccgca aggtcggcgc cgccgccgag
      721 cccggcaaga gcttcgagct ctggctgatt tcggacaagc tgccgcggcc gcgctcgctg
      781 ggtgtgatcg gcggcgccga cttcaccgcg cgcccggtgc tttcgtccta cgataccgac
      841 accatcaaca ccgcgaccta tgcggtgacg gtcgagcagg ccggcggatc gcctgacggt
      901 aatccgcact cggcgccggt ctttaccggc aagctgatcg agagcgttcc gtcagcggct
      961 ccggcgcctt ctccagcccg ttagaggccc gtgccacatg cggcgacgcc acctcgccgc
     1021 agggcgaagc cgagccgttg cgcctcatga acaagaaagc aagccacaaa aagaaaacgc
     1081 ccgtggggag cgggcgtttt cggtagtcga cttggggtag ttgggtcttc tacatatcca
     1141 cgtgacgact ttggggggct gctaaagggt cgcgtgaatt cacaaaactc atcagcggac
     1201 cgcggatgta ttggcgctcg tgaccgtcac ggccttgccg gccttgataa cgcgcgcggt
     1261 ctgggtatat ccgtcgtccg aattgttgcg agccgagatg ccgaaaccag cgaccgcgat
     1321 acccgcgacg agcgcgacga ccacgatctt gagatgggtc gtgcgatctg cgctgtagat
     1381 cgaatggttc atcgaaggct cctgccgtcc cactgccgct tgcggattgg tcgatttcgt
     1441 ccaccggcag gttcaacatc tcaaccctgc aaacgtaggg aatcgagctt tagttccaaa
     1501 gaggcgatca caaggcagtg ataagacttg aattcccgcg actggctcta acgttaggat
     1561 taaaaactta ttgattattt agttattaca attagttaca atttatgcct gtcttgcgcc
     1621 gcaaaacggc gccaatcagc ccaggctgat aaatcatttg cctgtggcca aaaagccccg
     1681 tcgctggtgt gctgatttgc ccgggcacga gcgttttcaa gcgaacgcct tgccccggac
     1741 gtgatccgga gtggatatcg gttcgcacca ggaaaacgcg tcaaaacaaa aacgcttcaa
     1801 aacaagagat gagaacctcg attctgatgc aatcggaaac agggctctac tccagtctag
     1861 acgcttccga ccgtcttcag ccgcgcgcgg ggatgaattt cagcctgcga cagaaccgtg
     1921 gtttgcgacc tgaaccgctc caccagcgag cgcacgaagg ggcggatcgc ggccgaggtg
     1981 accaataccg gagactcgcc ttcgcgggcg gcctgttcga aacggtcacg caccgcggtc
     2041 atgaactcgc tcaacctgga cggctgcatc gcaaggctgc gttcttcgcc ctggccgacc
     2101 agcgactcgg caaaagcctg ctcccacttt gccgacagcg cgatcaacgg caggtagccg
     2161 ttcatcgagg tattctgtgc gcaaatctgt cgcgccagcc gcgcgcgaac atgttcgacc
     2221 accgccgccg ggttgcgcga gaacgccagc gcgtcggcga tgccttcgag aatggtggag
     2281 agatcgcgga tcgagatacg ctcggcgagc aacagctgca gcacgcgctg gatgcccgat
     2341 atcgtgacca tgctcggcac gatgtccttg accagttcgc cttgctcctt cggcagatcc
     2401 ttgaggagtt tttgcacctc gccataggac aaaaggtccg acatgttgtt cttgagcagt
     2461 tcggtgagat gggtcgagag cacggtcgcg gcgtcgacca cggtatagcc cttcaatgtg
     2521 gcctcttcct tcaggctggc gtcgacccag gtcgccggca gcccgaaggt cggctcgatg
     2581 gtgtgaatgc cgggcacccc gacctgattt ccggcagggt ccatgaccat gaactggctt
     2641 ggccagatct tgccggtgcc ggcatcgact tccttgatct tgatgatgta agtgttggcc
     2701 tccagctgca cattgtcgag aatccgcacc gccggcatca caaagcccat ttcgaccgcc
     2761 agcgaacggc gcagcgcctt gatctgctcg gtcaggcggt cggtaccgtc gggaccgttc
     2821 accagcggca gcagcgcata gccgagttcg atcttgagat cgtcgatctt cagcgccgtt
     2881 gaaatcggct cttccgcagc actcgcagca gccgcggctg ctgccggagc ggccgccgca
     2941 acggcttcag cctgcttggt ggcgcgattg cgtttgcgcg ccgaaaacgc cagcgctgca
     3001 gcaccactgc ccagcaggaa aaaagggatc atcggaatgc ccggcagcat ccccagcacc
     3061 agcatcacgc cagccgacat gccgagtgcc tgcggataac cggacagctg cttcatcagc
     3121 gccttgtcgg cggcgccgct gacgcccgct ttcgagacca gcaagcccgc cgcggtggag
     3181 acgatcagcg ccggcacctg ggtgacgaga ccgtcgccga cggtcagcag tgtgtaggtg
     3241 cgcgcggctt cgccgaaccc gaggccctgc tgggcgacgc cgataatgat gccgccgatg
     3301 acgttgatga acacgacgag caggcccgcg accgcgtcgc cgcgcacgaa tttcgatgca
     3361 ccgtccatcg caccgaagaa gccgctttcg tcctccagcg ccttgcggcg ctctttcgcg
     3421 accttctcgt cgatcaagcc ggcggagagg tcggcgtcga tcgccatctg cttgcccggc
     3481 atggcgtcga ggtgaaagcg ggccgcgact tccgcgatac gccccgaacc cttggtgatg
     3541 acgacgaaat tcacgatcac caggattgcg aagacgataa ttccgattac gaaattgccg
     3601 cccatcacga aattgccgaa ggcttcgatg acgtggccgg cggcggcggt gccttcatgt
     3661 cccctcgaca ggatcagccg cgtggaagcg agattgagcg acagccgcag catcgtcgaa
     3721 atcaggagga tggtcgggaa cgacgagaat tccagtggcg cctggatgaa cagcgatgtc
     3781 atcaggatca ggatcgacag cgtgatcgag atcgccagaa acagatcgag cacgatcgag
     3841 ggcaaaggga ggatcagcac caccaggatg gtgaggatgc cgaacgccag cgcgagatcg
     3901 ccgcgcttca ggatggtgcc gatttcgctg agacttggaa atccgccgcc ggtcgctccg
     3961 ccctgacccg ccgtgatatc gaccatggtt gctgcttccc cccgcatatg ccggtcgcgg
     4021 gcgccaaacg tcttggcggc ggccgaggga ccaccgtttc gaaagtgagg caccgcactg
     4081 gcgtccacgg gtactccccc gtcttacgcg gctgacgaca ctcaaacttc actaggcaat
     4141 ttttgcccgc tttatggtta ggaaagcgtt aacggccgtt gttgcgcttg tcattccgag
     4201 gcgatgcgaa agcatcgaac ccggaatctc gaggttccgg gttggctcgt tgcagtcgcg
     4261 ccccgtaacg acggacttga gccaaaatgt tcgcgccgac tgttctgtta cgctgcatct
     4321 gcatgcctaa tatcgcgcca tactgcttga ccaataggct gcgagggatg aagttgccgc
     4381 cgtgcacgcg ccctccgatt cctcaaaaga ttcagccgca ttcaccccgg actccggtca
     4441 ggcatgggtg aggcttggcc ttgcgctcat catcggctcg atcggcagcg tcgggatgtg
     4501 gtcggtcgtg gtggtgctgc cgggggtgca ggccgaattc ggcgcgaccc gcggcgcggt
     4561 ttcgctggcc ttcacgctgg tcatgctcgg cttcggtctc ggcggcgtcg ccaccgggcg
     4621 gatcaccgat cgcttcggca tcgtgttggc ggtggcgctc agcatctgct tcctcgggat
     4681 cgcctatgtt ctcgccggac tttcgaccac gctgtggcag ttcattgcgg tgcatttcct
     4741 gatcgggctc gggacatcgg cgaccttcgc gccgctgatg gcggaggtct cgcactggtt
     4801 cgaacgctat cgcgggctcg cggtgggcat tgtcgcaagc ggcaactacg tcggcggcac
     4861 gatctggccg ccgctggtca attggggaac gcaagttacc ggctggcgaa ccacccacat
     4921 cgccattggc attttctgcg tggtgatgat gacactcgtg ctgctggtgc ttcgcgcccg
     4981 gatcggcggg gcgacgctgc gagatcatga gaacgcgccg ccaccgcagg tcgatctcag
     5041 gctctcgacc aacacgctga cgacattatt gtgcgtcgcc agcatttcct gctgcgtggc
     5101 gatggcgatg ccgcaggtcc acatcgtcgc ctattgcggc gatctcggct acggcgtcgc
     5161 gcgcggcgcc gagatgctgt cgctgatgat ggcgttcggc gtcgtcagcc ggatcggatc
     5221 ggggtttcta gccgacagga tcggcggcat ccgcaccctg ctgatcggct cggtggcgca
     5281 gggctttgcg ctgttgttct acctgttttt cgatggcctg acctcgctct atgtcatctc
     5341 cgcgatgttc ggactgtttc agggcggtat cgtgccgagc tacgccatca tcgtccggga
     5401 atcgatgccg gcgcgggagg ctgcgacccg cgtcggcctc gtgattttgg cctcggtgtt
     5461 cggcatgtcg ttcggcggct gggtgtccgg cgtcatcttc gacgcgacag gctcctacgc
     5521 cgcggcgttc ctgaatggac tcggctggaa tgcgctcaac atcgccatca tggtgtcgct
     5581 gttgatccgc gtccggcagc ggctggcgct ggcgtaaggc ctgcctttgg cggaacgcac
     5641 gcgccgcaag agcccgttcc gatcgcgccg aatgacgacc gcataggacg gagcgtcgat
     5701 tatgcctttt cgacctcgcc gcctgcatcc agccagccct tgaagccgcc aaggttttgc
     5761 acgttggcgt atcccatctc cttgagcgtc ttgccgacca gcgccgagcg tccacctgac
     5821 gcgcagtagg caaccaccgt cttggcctga tcaaacgccg cgtcgtgcag cggcgaagcc
     5881 ggatcggcgc gaaattccac aagtcctctc gggatcgcga gagcgcccgg aacttttccc
     5941 gatgccgcta cctcggccgg ttcgcggacg tcgagaaaca acacgtcggt tcggccgagt
     6001 agcccttttg cgtcctcagg gctgatacgc ggcaccactg cttcggcctc ggccaacatc
     6061 gcctggactg tcttcatttt atctcctccc ggaacaacgc gcgcatgcga tcatgcgcgg
     6121 ggagtaaggc gcaaactttc gagcgttgca acgcaaaaat atgctgtgcg tcggcatcgt
     6181 gattccagac tcggtctcgg tttttgcacc gcgcggcggc gacttgcgat ggcgtaacgc
     6241 cgctagctcg acgcgatcgc cgcgcggatc gcactgatcc gggcttcgct gaacgcgccg
     6301 ctctcgagca actgcaggat cgagagcaca tcgcctcgcg atccccagct tccctcgtcg
     6361 atgatctgga acgtcaccca ccaggtcggt gacggcttcg gcagattgaa agcttccgcc
     6421 attcgcgcga gcacaccgcg aatgacgcgc cgccgcacct cggtcggcca ggcggcattc
     6481 atcaccgcaa tgtggatggt catgatgtcg cgcggagaat ccagttcggc gagaagcttg
     6541 ccgccgatcg ccatgcaatc ggccggcagc tcgcgaaaat gcacctgaaa gccagcccgc
     6601 gcggccgggg caaactgtcc gacttccggt tcgagtacgg cgtcggtcag ggtttcggca
     6661 aggcggccgc gctgcgcaag gctcagccgc ttttccggcg tggtcaccgt aatgatggtc
     6721 atgattcgat cccgcacccc gctatgacac gtggcatagt tcacatctgg gctggctatg
     6781 tcaattgtca taatctatgg ttggccggaa aaaacctggg gcaggcttgc gatgaattcc
     6841 gacacccgag cgaaaatggt tgccggcgca gccgaccttt tgagccggcg tggcgtcaac
     6901 gccaccagcg tacgtgaagt cgtccgccac agcggcacgc cgcggggctc gatcggccat
     6961 cattttccgc gcggcaagca gcagctgatc gaggacgcga ttgcgttcgc cggcgcagag
     7021 gtcagcgctc cgctggagca tctgatgcgt gaacggggcg cgatcgccgg cttgcgggcc
     7081 tttatcgcgc tgtggcggcg aacgctggaa gcgtcgcatt tcgaggctgg ttgcccggtg
     7141 ctggcggtgg cggtcgagca atatgtcagc gatgcgatgg acaagggcgg ggcgagggac
     7201 gaaagcgtgc agcagcatct gcgcgatctg gcacatggcg tctttgccga ttggcaaagg
     7261 atcatcgcga cggccttgct gcgcgaggga gcaggcgccg cgcgcgcaag acggctcgcg
     7321 gcgctggtgg tcgcgtccgt cgaagggaca gttgcgatgt gtcgtgccgc gcgtagcgcg
     7381 cggccgctcg aggatatccg caaagaactc gaactcatct tgtccgcgtc gataccgaag
     7441 tgagggcgcg cccgcggctg gcgatggcgt gagcgtcaca ccccctggcc cgccttgagc
     7501 aggacgcagc ctgagatctt gacgctgccg tcgggctcca gttccagcgt atacagcgcc
     7561 tcccacggca cgccttcggc gtcgatgatg tgaacgcgct gggcgatctg gccgcgcgag
     7621 acgcgcgcct caccgaactc aaagctcttg tggcgataga ccggggcata gctctggcgc
     7681 actatgtcca tgaagatgtc ggcttgcgga aacatgtccc ggattgcggg cgccgcaaaa
     7741 gagtaggccg ttgcggcatc gtcgcggcct agcgcttcga cctgggaacg gatgacggtc
     7801 tgcgcggcgg caacatcatc cgcggcgcgg gcgggggagg cgaggctgga gcaggaacgc
     7861 ggtcagcagg acaagtgcgc gcatgatagg gatccttgtt cgctcgttcc tcttacgctg
     7921 caatccttcg aaggtcatgc cgcgaaacgg cggcaattgg tgggcgcggc agtagccgac
     7981 cctgttcctc ctcggagcga atcacgccat tgatgacaag gcctttgaag gttcagggca
     8041 attggccaag cgcctcgcgc atatcagcga ccgaggcggg ttcggcgccg tgatcgcgca
     8101 ctatccgcac ggcttcctcg accaaggcga gattgctcag cggcgtgcgc agcggcgcgt
     8161 cctcgaggcc gacacgaata tgcccgcccc gcgccaccgt ctcgccgatc agcgggcggg
     8221 gatcggcgca tacgcctgcg atcatccacg gcgccaggcc cgcctcctct tccagcagcg
     8281 ccagatgggc ggcgagcgcg tagggcctgg gcggaaagcc gacggcaatt gtttcgcaga
     8341 acatgaatcg atagatcggc gtcttgacgc cggccgcgcg cgccagtgcg gcgccggcgc
     8401 gcgtgaagcc gggttcgtag atcgcaaacg ccgggtgaaa gccgtggcgc gccgcaaact
     8461 cgagcgcatg ccggacatgg ctttccggat tcatgtaggt ggcggctgga cttgcggccg
     8521 gtgtcgtctt cgtccggctg aaattgacgc tgcccgggtc gataatggca aaatcgagca
     8581 gaccgcgcgc ggccagcgct tcgatatggg cgaagcgaac ggtggcatcc gcgaaggagg
     8641 ggcctgtatc ggcggctgtc gtcatgaacg cggggtatga cggataaacc ggaacgtcca
     8701 cttgcgcccg aatgccctcg atgatgcgcg cgtaaacctg ccagtcaaag gtttgcaggc
     8761 cgccgccgtc ataggcatgg gtgtgaatga tggtggcgcc ggcgcgcgcg caggcgacgc
     8821 cgtcggcgat gatcgcctcg atcgtgtcgg gaatgcccgg ctgcagtgcg cggctccagg
     8881 cgccgttcag cgccgcttcg atccagacct tgcgcatcgc ggtatctcct tttgcttttc
     8941 attcggcacg ccctgcggcg cgagcgacaa tccgccggcg ataaaactcc acaggcgatc
     9001 cgccagttga tcgggatcgg gccgccggcg cgatgagatc gcccgcgcga cggcctgatt
     9061 gacgccgccg ataatgacgg cgcccgcgat atcgggatcg atatcggccg caatgaatcc
     9121 tcgccgctgg ccctgggcga cgtttcgcgc ggccatctca accacggcgg atcgccgccc
     9181 ggtgtcgatc gcgatcacct ccgcactgct gctcagtttg cccatcatca ccggggcgac
     9241 gggatcggca tacatgaaat cgatactggc cttgagccgg gctttttccc gcaccggcca
     9301 cggcagcgac tcgtcgaagc tttgattgaa gacggcctcg tggcggttgt gaaactccgt
     9361 gatcagcacc gagatcaacc cggctttcga tccgaagtaa tggtaggcga gcccgaccga
     9421 cactttcgcc cgccgcgcca cgtcgccgat ctcgaaatcg ccgtcacctg cgaccagctc
     9481 ctcgagcgcg gcggcgacga tgcgggcgcg ggcaccgttc tcacggctgt acgtgccggt
     9541 gttcttgccg ctgtccttcg gcgtcgttcg gcgaaccggt cgtggcacca tcttgactcc
     9601 tgaattgaat tcaattcaat atagcggacg gagcacggga tggaaatgtt ttttggcgag
     9661 gagaaagcca atgccgacaa caatcgagca ggcatcgccc tggctgcagg gacgaacctt
     9721 cgcatcgtgg cgggaagagt tcgatcatcg cggctatctt tttttcgagc gggttctttc
     9781 tcccgaccgg gtgacggcaa tccgcaccgc gctggcgccg tatcttgagc gcgatctcac
     9841 cggccgcaac gatttcgagg gcctgaagac caaccgggtc tacgccttga tggcgaagtc
     9901 gccgctattc gcagagcttg cgatccatcc gctggcactg gcttttgccg aagccgagct
     9961 cggacaaagc tgcctgctgt cggccctgct tgcgatcaat ctgcatcccg gcgaaaccgt
    10021 gcagccgtgg catttcgacg acggcggcgt caagctgccg cggccgcgcc cggccttcgg
    10081 cgtcagcgcg ttctgggcga tcgaccacac taccgaatcg aacggcagca ccgaagtcat
    10141 tccgggaagc catctgtggg acgggcggat cgtcgagggg gcggtgaagc ccgccgactt
    10201 tgccaacacg gccgcgccga atcccgacag cgatttcagc aaccggccgg acgccgtaaa
    10261 actcgtgatg ccatcgggat cgctggcgat cacgaagggg accctttggc atcgcggcgg
    10321 cgccaatctg tcggaccggc cgcgtttgat catcacgccg caatattgtg cgggctgggt
    10381 gcggcagctt gagaatatgg ctctggccgt tcctgccgcg ctcgcggaaa aattaccgga
    10441 gcgtgcccgc gaactgatcg gctattcgat ccaccctccg ttcatggggc acgtcgatgg
    10501 cgttcacccc aagcgactgt tgcgatcgcg ggtgacgtga tgtgggagag aaaaccatcg
    10561 ccaaagcgcc ctctgaatca gtgtttggac tccagttcga tgatggcgcc agtcggctca
    10621 ggttcgtgag gcgccaggct cgtaagagcc tcgtcgctcc aactcgcaag attgacgact
    10681 ctcgctgcct gaactcgatc tcgaggagac tggtcggccg gctccaaaat gttcagtcca
    10741 ttgatatcga gcagaaaagt gtgatcccca aacaattcgt tcagttgcgg aaccgctggg
    10801 tgattgtcgg gaagggcttg ggcttcgaac tggttgatgg ttcgctcgac ctgtgcggaa
    10861 gtcaatttca tcgctcattc ctttcaattg gtttctcgtg ttgcaacgcc aaggcgctgc
    10921 gggtcacgtg gtgcatgccc tgccaccttc aaggtggtca aacgcgggtc gatatttctc
    10981 gtgcaaaccc acgccggtgc atcttcgagc gagcagctaa agccgcggcg aacccggtac
    11041 tcaatgtacg ccggagctgt gcgtgggaat cgattgcggc ataccgtccg caatacctac
    11101 actatgttga tcttcctttt tgaccgtttt ttgtctccac gaactttaat tgatcggtat
    11161 gccttctcga catatcaggt cgcctgaatt ccggagggaa cccggcacag ccaacggtca
    11221 cttttattgt ttacaaggcg tcagagtttg aactgccgtt tcggcttcgg cccgcgtctt
    11281 ataaatcgtg tcgctggcaa cggtgatgtc cggcgtattg gtacgaggca tcctgtcaac
    11341 gaccctgcag tttttggtca aggaattcag cacaatgtaa aatacaggtg ttgctttcgc
    11401 gatctgagag tggccgggac tgcccaagat catcagcaaa cagcagcaga tcaaacttcg
    11461 tttcatcgcg gtttcggtca tcaactaaga taacctccaa gcttggaatt gagttcccgg
    11521 caagatgaat cggaacttga cgagacgggg tgagaattcg ggcctcagcg tcccggtccg
    11581 gtcactcgcg ggcggactga acgcgaggat ttatccaaat gattgggccc cggtgcatca
    11641 aggcatcgcg gggcccgtaa tcttcgtctt gcgaagaatg tctggtacga accagttgta
    11701 tgttgagatc tcagaacgcg tggcctttgc gcctgggtct cgcggatatg ttcagattgt
    11761 cggcggcgca aatgacggct agcagctgct gttcacctca acctgcgccg tgaccttgca
    11821 gtcgagcaag gtcaatgctt catgcccggt ggcgtcatgc tcctcaccaa gtgtgaaccg
    11881 gcggacattg tgtccgcgtc cgctgcgcgc cgtctcgacc acatgatcag acgccatgcc
    11941 tacgaagccg ggagccaaat actccacgag cttcgacagg tgcagcatcg gaaatgctgc
    12001 ggcagatgtg caccggagtc aagttacact gacggcaaac cggccactcc cgtttgggaa
    12061 ccgcagtgca aatctgcaaa aaaaggtggg cccggtggaa caacatcggg ttttgaaatt
    12121 cgctgcgccg tgcgccacag gtttgtccga acgccgttct ggaagcgatc ttatgggacg
    12181 attgcgcgca tgaaagctcc ggggttgatt gttcaggcca caaaattgca ggagcgcgcc
    12241 cgcgaaccga tcggctattc gatccatccg ccgttcatgg ggcatgtcga tggcgttcgc
    12301 cccgggcgtt tgttgcagcc gcaagccgcg tgatacgggt gagagggtct tcgcgcgcgc
    12361 ggtctccctt tcgttctcat tcgaacgtat gacatcgggc aatgctggtc tctgtcagct
    12421 ggtcgcattg tggctttcgg ccattcactt gatggcgatg tttgccatca cgtgacgaca
    12481 tcgaaataat tgagctttga aagttcgtcg ccctcgacta tcaagcgcac gtccaagcgg
    12541 accaaccgcg tggtttccgg agatcctcat gcccattcgc tgcctgctcg ccacctcgct
    12601 cattttgagc tgcttgtcgg tggcgccgat gaaggccgcc gagaccgtca cgtcagcagt
    12661 tccgggcagt cgaacgacga cctacaagac cgtcgccgtc aacggcctga acatcttcta
    12721 tcgggaggcg ggcccgagca acgcgcccgc gatcctgctg ctgcatggct t
//
LOCUS       DATNOI010000511         9710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1650015,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000511 DATNOI010000000
VERSION     DATNOI010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9710
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650015"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VN968_20535"
     CDS             <1..784
                     /locus_tag="VN968_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL31657.1"
                     /translation="LFEPSVEGYVNLFTTRSRQTPEFLASLPPPQTWYERLVRSRNMV
                     IGGPSKVVPRFLNSMIIGFGSTFLAVCFGTIAAYAFSRFRVPLADDLLFFILSTRMMP
                     PIAVAIPIYLMFRYLGLSDTKLGMILLYTAVNVSLAVWLLKGFMDEIPREYEEAAMVD
                     GYTRLQAFFKVVLPQATTGIAATAIFCLIFAWNEYAFAVLLTSGEAQTMPPFIPFIIG
                     EGGQDWPAVAAATTIFFIPILCFTILLRRHLLRGITFGAVRK"
     gene            781..984
                     /locus_tag="VN968_20540"
     CDS             781..984
                     /locus_tag="VN968_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31658.1"
                     /translation="MTERIARHASRWPLWLRRGRCEVTAMALIGLGVLMLMQPLSLNL
                     YSYSFVTILAGTLGFVIVSHFPD"
     gene            990..2087
                     /locus_tag="VN968_20545"
     CDS             990..2087
                     /locus_tag="VN968_20545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016555187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31659.1"
                     /translation="MAEIKLETVAKTFGTFTAVKGVDFTVDHGSFFVILGPSGCGKTT
                     TLRMMAGLELPTAGRILLDGEDVTALPASARDIAFVFQLFALYPHMNVRQNIAFPLRC
                     QNYPRAEIRRSVEETARLLRIDHLLDRRVGGLSGGDRQRVALGRAIVRRPKAFLMDEP
                     LGALDSEFRRLMCGELRELHDRIDATTVYITHDQLEAMAMADRIAIMNQGRVEQIGTP
                     QEIYDRPASMFVADFIGSPPMSFLSVQATLQRGDRCIKVDGAPVEMPQLFDDRAKGSF
                     ALGVRPENVSFADASRLRGRVIGAEYLGTTQIVTVTTANGQIKARLPSGNTVRVGETV
                     GLALRPDKLSLFDAISGLALPSALYAGDRDG"
     gene            2080..3114
                     /locus_tag="VN968_20550"
     CDS             2080..3114
                     /locus_tag="VN968_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007814751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31660.1"
                     /translation="MAEVALLDVSKSFGAVEAVRGLSLSVAHGEFVVLLGPTGAGKTT
                     TLRLIAGLERPDQGDVVIQGRVVTREIPAERDVAFVFQQYSLYPHLTVYDNLAFPLRS
                     PARRTDEKTIRRRVQQISELLHIESKLGNRATALSGGEMQRVAIGRALVREPAVYLMD
                     EPLSSLDAKLRAELRLELKRIQRELGTTILYVTHDQIEAMTMADRIGVMSEGALIQLG
                     TPREIYERPNSAYVAGRLGTPVINLIPAELLGNTLPPLVRTVGLRTEHIRISPADGPG
                     PRAQVHWVEHLGDQNHVHLKLATHSLVTLADPGQPLRAGDRVSLEFESPLYFDQAGNR
                     LGTDGLRGHA"
     gene            3111..3725
                     /gene="dhaL"
                     /locus_tag="VN968_20555"
     CDS             3111..3725
                     /gene="dhaL"
                     /locus_tag="VN968_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HXL31661.1"
                     /translation="MMIDRQARKLLFETVARRVIASTDELTDLDRAIGDGDHGANMRR
                     GFEAVLAAVDELAAKNLGESLKGVGTTLVMKVGGASGPLYGTLFMSLGKTLDDEVTCE
                     RVADAFAAAIEAVKARGKSDVGQKTMLDVLFPVLAVLREGGTELPVRLKAVAKAAAEE
                     TIPMRAIRGRASFLGERSVGHMDPGARSSALIVDAVADVMEGLA"
     gene            3722..4114
                     /gene="dhaM"
                     /locus_tag="VN968_20560"
     CDS             3722..4114
                     /gene="dhaM"
                     /locus_tag="VN968_20560"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004434253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HXL31662.1"
                     /translation="MSENVGIVIVSHSADVARGTALMVEQMVGHEVPLGWCGGDPGGG
                     LGTSVEAIMAAIDQAWSERGVAILVDLGGAETNSEMAIEMLPEARRARVVVCNAPVVE
                     GAVMAATEAASGASLEAVRRTAEELTPV"
     gene            4130..4411
                     /locus_tag="VN968_20565"
     CDS             4130..4411
                     /locus_tag="VN968_20565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HXL31663.1"
                     /translation="MNKLRASVLMRHEVGLHARPSVKLTKLAKGFESRIDLGLSPDGP
                     WIDAKSIVKVMATKAPKDSTIYFRAEGADAKAALEALVTLVDGDFQDGR"
     gene            4401..6011
                     /gene="ptsP"
                     /locus_tag="VN968_20570"
     CDS             4401..6011
                     /gene="ptsP"
                     /locus_tag="VN968_20570"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HXL31664.1"
                     /translation="MDDRTHRAAHRIQGRSAAPGIALGPLVRLAPVEHGVRQYRSAAE
                     GRQALLDALAASQEDLSALAHQAGDDEAEAILAFQIALLEDDSLIAPALARIDVGDGA
                     NQAWSAAIDPEIASYDAAEDPYFRARASDLRDLRDRVLRHLAGETDQIVPPGVILAAD
                     DMPPSTFLATDWQDGGLVLRRGSPSSHVAILARSRGVPMIVGVDVDHLEDGADALLDA
                     DAGLLVVDPDANMRASFDQRRAEQSKARQAAASFCGPALTATGELVRVMINVTGLAEI
                     EGLDPAHVDGIGLMRTEFLFQGREKLPTEEEQYQIYKRMLEWAAGRPVTIRTLDAGGD
                     KPIAGLTQPGEMNPFLGVRGVRLSLRRLDVFREQLRALARAAVAGNLKVMIPMVTVPE
                     ELDRCRTLLEQALEELRREGLEAQLPPLGMMVEVPAAALTIEDFNADFFSIGSNDLIQ
                     YVAAASRDEPQLADLARPSRAVFNLVRHVVNYADSCGREASLCGDLAGDPAQVATLLD
                     QGLRIFSVTPGAVGPVKAAIARYSGSAA"
     gene            6008..6427
                     /locus_tag="VN968_20575"
     CDS             6008..6427
                     /locus_tag="VN968_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31665.1"
                     /translation="MSTQATPAGSQDLIAAYKAILRDVLDRRPSGMRQRLAEALGKNR
                     SFITQIANPAYQTPIPAQHVHPIIQICHFSVQERDRFLEAYHRAHPRRLLLLKERERS
                     RRLTLMLPDLGSEQRNHKLDSLLSEFAEKVARLIEDT"
     gene            6459..7448
                     /gene="dhaK"
                     /locus_tag="VN968_20580"
     CDS             6459..7448
                     /gene="dhaK"
                     /locus_tag="VN968_20580"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HXL31666.1"
                     /translation="MKKLINAAENVVSESLAGFCAAHSDIVRLGEHAPFVQRRHLKPG
                     KVALVSGGGSGHEPLHTGFVGYGMLDAACPGQVFTSPTPDLILAAAEAADTGAGVLLI
                     VKNYEGDVMNFDMAREMSGKSIATVITDDDVAVETSTFSTGRRGVAGTLIVEKIVGAA
                     AEEGRDLAALKLLGDRVNAATRSIGIALTSCTVPAAGRPTFDIAEDEMEVGVGIHGEP
                     GRRRVKLRPAAEIAAEMVRAITTDLGERVRGEAILLINGFGATPLSELYLMYDAVRRL
                     LEPADTIVTRSLVGNFVTSLDMAGCSVTVSMLDDEMKRLWDAPVHTPALRWGI"
     gene            7540..8214
                     /locus_tag="VN968_20585"
     CDS             7540..8214
                     /locus_tag="VN968_20585"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXL31667.1"
                     /translation="MKPDLIIFDCDGVLIDSEVLSCRCLSEVLARYGVSLGLDQALDL
                     FLGRSATAVLDHYQALGRSIPEQFYMELRTAVRAAFLAALCPIEGVRSVLEGLQVPYC
                     VASSSDLDRVSFSLSLTGLAPHFGSRLYSAQMVERGKPAPDLFLYAAERMQAHPCNTL
                     VIEDSVSGVTAAKAAGMTVWGFVGGSHYQSRDGKAILLDAGADRVFERMADFWPNESE
                     EFYGRA"
     gene            8201..9154
                     /locus_tag="VN968_20590"
     CDS             8201..9154
                     /locus_tag="VN968_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HXL31668.1"
                     /translation="MVAPDSEKSRLDEAARAGWLYFIAGHTQDEIAKMLQVSRASAQR
                     LVSLCLAERLITFRLEHPIAACMELAARLKDLFHLVYCEVVPNDPAAQLSATGIAERA
                     ANILESTLRTEKPAIVALGTGRAVRAAVERVSPTDCPNHQIVSLVGNISADGSASFFD
                     TVGRLADRTKARHYPMPLPFLMSSEHERDQMLRLDPIARVRAVAAKADLRLVGIGQMD
                     QRAQVFVDGFVSREELLEMMRLGAVGELTGWAFDDQGHIIDGGTNRRLTSIPPRVPAE
                     ALTIGAAVGRAKVSAIRAALKGRLLNGLITDEATAGAILKP"
     gene            9354..>9710
                     /locus_tag="VN968_20595"
     CDS             9354..>9710
                     /locus_tag="VN968_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXL31669.1"
                     /translation="MKHILGALLGAVTLLGSGPSIAQTTLTIATVNNGDMIRMQALTN
                     EFTAKNPDVTVKWVTLEENVLRQRVTTDIATKGGQFDVLTIGTYEVPIWAKKNWLIPL
                     DNLGADYDAADLLPRIS"
BASE COUNT         1761 a         2917 c         3118 g         1914 t
ORIGIN      
        1 cctgttcgag ccttcggtcg aaggttacgt caacctgttt acaacgcgct cgcggcaaac
       61 gccggaattc ctggccagcc tgccgccgcc gcagacctgg tatgagcgcc tggtgcgctc
      121 ccgcaacatg gtgatcggcg ggccgtcaaa ggtggtgccg cgctttctga attcgatgat
      181 catcggcttc ggctcgacct ttcttgccgt gtgcttcggc accatcgctg cttatgcgtt
      241 ctcgcgcttc cgggtaccgc ttgcggatga tttgctgttc ttcatcctgt cgacgcggat
      301 gatgccgccg atagctgtcg cgatcccgat ctatctgatg ttccggtatc tcggactaag
      361 cgataccaag ctcgggatga tcctgttgta caccgccgtg aatgtttccc tggcggtatg
      421 gctcttgaag ggtttcatgg atgaaatccc tcgcgagtac gaagaggccg cgatggtcga
      481 cggctacacg cgccttcagg cattcttcaa ggtggtgctg ccgcaggcga cgaccgggat
      541 cgcagcaacc gcgatcttct gcctgatctt cgcctggaac gaatacgcct tcgccgttct
      601 gcttaccagc ggtgaggcgc aaacgatgcc cccgttcatc ccgttcatca tcggcgaggg
      661 tggtcaggac tggcctgcgg tcgccgccgc gacgacaata ttcttcatcc cgatcctgtg
      721 cttcaccatc ctgctgcgtc gccatttgtt gcgtggcatc acctttggag ccgtgcgcaa
      781 atgaccgaaa ggatcgcacg acacgcatca agatggccgc tctggctccg tcgtggccgt
      841 tgcgaagtca ccgcgatggc gctgatcggt cttggcgttc tgatgctgat gcagccgctc
      901 tcgttgaacc tgtatagcta ttccttcgtg acgatcctgg ccgggacgct gggatttgtg
      961 atcgtcagtc attttccgga ctagccgcca tggcagagat caagctcgaa accgtagcca
     1021 agacgttcgg aacttttact gccgtcaagg gcgtggactt cacggtcgat cacggctcgt
     1081 tcttcgtcat tctcggcccg tcgggatgcg gcaagacaac gaccttgcgc atgatggctg
     1141 gccttgaact accgaccgcc ggccgcatcc tgctcgatgg cgaagacgtc accgcgctgc
     1201 ccgcgtcggc acgggacatc gcctttgtgt tccagctatt tgcgctctac ccgcatatga
     1261 acgtgcgcca gaatattgcc tttccgctgc ggtgccagaa ttacccgcgc gccgagatca
     1321 ggcgaagcgt cgaggaaacg gcaaggctgc tgaggataga tcatctcctg gaccgtcggg
     1381 tcggcgggct ctccggcggc gaccggcagc gcgtcgcctt ggggcgcgcc atcgtgcgtc
     1441 gacccaaggc gtttctcatg gatgaaccct tgggcgcgct cgattccgag ttccgccggt
     1501 tgatgtgcgg cgagttgcgc gagctgcatg accgcatcga cgcgaccacc gtttacatca
     1561 ctcacgacca actcgaggcg atggcgatgg cggaccgtat cgccatcatg aatcagggtc
     1621 gtgtcgaaca gatcggtacg ccccaggaga tctacgatcg tcctgcttcg atgttcgtgg
     1681 ccgacttcat cggctccccg ccgatgagtt ttctcagcgt ccaggccaca ctgcagcgcg
     1741 gcgatcgctg tatcaaggtc gacggcgcgc cggtcgaaat gccacagctg ttcgacgatc
     1801 gtgccaaggg ctccttcgca ctcggcgtaa gacccgagaa cgtatccttc gccgatgcct
     1861 cgcgcttgcg aggccgtgtg attggcgctg aatacctcgg caccacgcaa atcgtcacgg
     1921 tgacgactgc caacggtcaa ataaaggccc ggctgccgtc aggcaacacg gttcgagtcg
     1981 gcgaaaccgt ggggctcgcg ttgcggccgg acaaactatc cctgttcgac gccatcagcg
     2041 gcctggcgct gccctcggcg ctttatgcgg gagatcgcga tggctgaggt tgcgctgctc
     2101 gatgtcagca agagcttcgg tgccgtggaa gcggttcgtg gcctttcgct ctccgtcgcc
     2161 cacggtgaat ttgtcgtgct gcttggcccc accggtgccg ggaagacgac gacgctgcgt
     2221 ttgatcgccg gcctggagcg gccggatcaa ggcgatgtgg tgatccaggg tcgcgtcgta
     2281 acgagggaaa tcccggccga acgcgacgtt gcgttcgtgt ttcagcaata ttcgctttat
     2341 ccgcatctga cggtatacga caatctggcc ttccccttac gctctccggc ccggcgcacg
     2401 gatgagaaaa cgatacgccg tcgggtccag cagatatcgg agctgcttca catcgagagc
     2461 aagcttggca atcgggctac cgcgctgtct ggcggcgaaa tgcagcgggt tgctatcggc
     2521 cgggcgctgg tccgggagcc tgctgtctat ttgatggacg agccgctctc ctcgctggac
     2581 gccaagcttc gcgccgaatt gcgcctcgag ctcaagcgca tccagcgcga gttgggaacc
     2641 acgatcctct atgtgacgca tgaccagatc gaagcgatga caatggcgga ccggatcggc
     2701 gtgatgagcg agggggcgct cattcagctc ggcaccccgc gcgagattta tgagcggccc
     2761 aacagcgctt atgtggccgg ccggcttggc acgccggtca tcaacctcat tcctgccgag
     2821 cttctgggca atacattgcc gccgctggta cgaacggtgg ggttaaggac cgagcatatc
     2881 cgcatctcac ccgcggatgg tccgggacca cgcgcccagg tgcactgggt cgagcatctc
     2941 ggcgatcaga accacgttca tctcaagctc gcgacccact cgctcgtgac gcttgccgac
     3001 cctggccagc cgctcagggc gggggatcgg gtctcgctgg aattcgagtc cccgctctat
     3061 ttcgatcagg ccggtaaccg cctcggtacc gatggattgc gggggcatgc atgatgatcg
     3121 atcgtcaggc gcgcaaactc ctgtttgaaa cagtggcgcg gcgtgtcatt gcgagcaccg
     3181 atgaattgac cgatctcgat cgcgcgatcg gcgacggcga ccacggggcc aacatgcgcc
     3241 gcggatttga agccgtgctg gctgcagttg atgagctcgc cgccaaaaat cttggcgaat
     3301 cgctgaaggg cgttgggacg accctggtca tgaaagtcgg cggcgcatct ggcccgcttt
     3361 acggcaccct gttcatgtcg ctcggcaaga cgctggacga tgaggtgacc tgtgagcggg
     3421 ttgctgacgc gtttgccgcc gcgatcgaag cggtcaaggc gcgggggaag tccgatgtcg
     3481 ggcaaaagac aatgctcgac gtgctttttc ccgtgctcgc ggtgctgcgc gaaggcggga
     3541 cggagctgcc ggtacgcctg aaggctgtgg ccaaggctgc ggccgaggag acgattccaa
     3601 tgcgagcgat acgcgggcgg gcatcgtttc tgggcgagcg cagcgtgggt cacatggatc
     3661 cgggggcacg ttcctccgcg ttgattgtcg acgcggtcgc agacgtcatg gaaggattgg
     3721 catgagcgaa aatgttggaa tcgtcattgt gtcgcactcg gcggacgtgg cacgaggcac
     3781 tgcgctcatg gtcgagcaaa tggtcggcca cgaagtgccg ctcggctggt gcggcggcga
     3841 tccgggcgga gggctgggta ccagcgtcga agcgattatg gccgcgatcg accaggcctg
     3901 gtcagaaaga ggtgtcgcca ttctggtcga cctcggtggc gcggaaacca atagcgagat
     3961 ggcgattgaa atgctgcccg aagcgcgacg cgcccgcgtc gtggtttgca acgcgccggt
     4021 tgtcgagggc gcggtgatgg cagcgactga agcggcttcg ggagcttcgc tggaggcggt
     4081 gcgtcgcacc gccgaagaac tgacgccggt ctgatgcagg gcaagggtta tgaataaact
     4141 tagggcatca gtgctcatgc gacacgaggt agggctgcat gcgcgcccgt cggtcaaatt
     4201 gaccaagctc gcaaaaggct ttgaatcgcg tatcgacctt ggcctgtcgc cggatggtcc
     4261 ttggatcgac gccaagagca ttgtcaaggt catggctacc aaggcgccga aagattcgac
     4321 gatctatttt cgtgcagagg gggccgatgc gaaggccgcg ctggaagcgc tcgtgacgct
     4381 cgtcgatgga gattttcagg atggacgata gaacgcaccg cgctgctcat cgcatccagg
     4441 ggagatccgc cgcacctgga atcgcgttgg gaccgctcgt gcgtttggcg ccggtcgagc
     4501 atggcgtccg tcaatatcgg tctgcggcag aggggcgcca ggctcttctc gatgcgctcg
     4561 cagcgtcgca ggaagatctc agcgcgctcg cgcaccaggc gggcgatgat gaagcggaag
     4621 ccattctcgc tttccagatt gcgctgctcg aagacgacag cctcatcgca ccggcgttgg
     4681 cgcggatcga cgtcggcgat ggcgccaacc aggcgtggag cgcggcgatc gatccggaaa
     4741 tcgcgtcgta tgatgcggcc gaggatccct atttccgcgc gcgcgcatcg gatttgcgcg
     4801 atctgcggga tcgcgtgctg cgacatctgg ccggtgaaac cgatcagatc gtgccgccag
     4861 gcgttatcct cgcggccgac gatatgccgc cttcgacctt cttggcgacc gattggcagg
     4921 atggcgggtt ggtattgcgc cggggaagcc cgagcagcca cgttgccatt cttgcccggt
     4981 cgcgcggcgt acccatgatt gtgggcgtag acgtcgatca tctcgaagat ggcgccgacg
     5041 cgctgctcga cgcggatgcg gggcttctgg ttgtcgatcc cgacgccaat atgcgagcca
     5101 gctttgatca gcgtcgcgcc gagcagtcga aagctcgcca ggccgcggct tcattctgcg
     5161 gcccggcgct tacagccact ggtgaactgg ttcgggtcat gatcaatgtc accggccttg
     5221 ccgagatcga aggcctcgat cctgcccacg tcgacggcat cggcctgatg cggaccgagt
     5281 ttctgtttca gggtcgcgaa aaacttccaa ccgaggaaga gcaatatcag atctacaagc
     5341 gcatgcttga atgggccgcc ggcaggcccg tgacgatccg caccctcgac gccggcggcg
     5401 acaagccgat cgcgggtttg acgcaacccg gcgaaatgaa tccatttctt ggcgtgcgcg
     5461 gcgtgcggct gtcattgcgg cgtctggatg tgtttcgcga gcagttgcgg gcgctggctc
     5521 gcgccgcagt agcaggaaac ctcaaggtga tgattccaat ggtcacggtg ccggaggaac
     5581 tcgatcgctg ccgcacgctt ctcgagcaag ccctcgagga actgcgtcgc gagggcctgg
     5641 aagcgcaact gccgccgctc ggcatgatgg tcgaggtgcc ggcggcagcg cttaccatcg
     5701 aggacttcaa tgccgacttt ttttccatcg gcagcaacga tcttatccag tatgtggcgg
     5761 cggcgagccg ggacgaaccg caactggctg acctggctcg tccttcacgc gcggtgttca
     5821 atctggttag gcatgtcgtg aattatgcgg atagctgcgg ccgcgaagcc agcttgtgcg
     5881 gtgaccttgc gggcgatccc gcgcaagtcg cgacgcttct cgatcagggc ttgcgcattt
     5941 tttcggtaac acccggggcg gtggggcctg taaaggccgc gatcgcccgc tattccggtt
     6001 ctgccgcatg agcacgcaag caacgccagc gggctcgcag gatcttatcg cggcctacaa
     6061 ggcgatcctt cgggatgtgc tcgatcgacg gccgtccggc atgcgccagc gtttggccga
     6121 agccctcggc aagaatcgca gtttcatcac gcaaattgcc aacccggcct atcagacgcc
     6181 gatccctgcg caacacgtgc accccatcat ccagatctgc catttctcgg tacaggagag
     6241 ggaccggttc cttgaagcct atcaccgggc gcacccgcgc cggctgcttc tcctgaaaga
     6301 gcgcgaacgc agccggcgtc tcacgctgat gctgccggat ctggggtccg aacagaggaa
     6361 ccacaagctt gactcgctgc tgagcgagtt cgcggagaaa gtcgctcggc tgatcgagga
     6421 tacctgacgc cgaagcgatc gcaaacgggg agggtaggat gaagaagctg atcaacgccg
     6481 ccgagaacgt ggtgagcgaa agcctggccg gattctgcgc ggcgcattcc gacatcgtgc
     6541 gcctcggcga gcacgcgccg tttgtacagc gccggcatct caagcccggc aaggtggcgc
     6601 tggtgtcggg tgggggctcg ggacacgaac cgctgcacac cggctttgtc gggtacggga
     6661 tgctggatgc cgcgtgcccc ggtcaggtct ttacctcgcc cacgccagat ctcattctcg
     6721 ccgccgccga ggccgcggac accggtgcag gcgtgttgct catcgtcaag aactatgagg
     6781 gcgacgtcat gaacttcgac atggcgcgcg agatgtcggg aaaatcgatt gcaacggtga
     6841 ttaccgatga cgatgtcgcg gtagagacct cgaccttttc gaccggacgg cgcggcgtcg
     6901 ccggcacgct cattgtagag aaaatcgtgg gagcggctgc ggaagaggga cgcgaccttg
     6961 ctgcattgaa gctcctgggc gatcgcgtca acgcggccac gcgttcgatc gggatcgcgt
     7021 tgacgagttg cacggtcccg gcggccggca ggccgacctt cgacattgcc gaggacgaga
     7081 tggaggtcgg tgtaggcatt catggcgagc cggggcgtcg ccgcgtaaag cttaggccgg
     7141 ctgccgagat cgcggccgag atggtccgcg caattacgac cgatctgggc gagcgcgtac
     7201 gcggcgaggc aattctgctc atcaacggct ttggagcaac gccgctctcg gagctctatc
     7261 tgatgtacga tgctgtccgc agattgctcg agcccgccga caccatcgtt acgcgatcgc
     7321 tggtgggtaa cttcgtcaca tcgctcgaca tggccggttg ctcggtcacg gtgtcgatgc
     7381 ttgacgacga gatgaagcga ctctgggatg cgcccgtcca cacgccggct ctgcgctggg
     7441 gtatctagca gatcagaaat gcgcaattta tgtgcatgat cggggttatt gcccaatctc
     7501 tgagtatatg ctctgattga gattgggaaa cttgcgatga tgaagcccga tctcatcatt
     7561 tttgattgcg acggcgtgtt gatcgatagc gaggtactga gctgccgctg tctttcggaa
     7621 gtgctggcgc ggtacggcgt cagcctcggt ctggatcagg cgcttgatct gttcctcgga
     7681 cgaagcgcga cggcggtcct cgatcactac caggcgctgg gacgctccat tccggagcaa
     7741 ttttacatgg agctgagaac cgcggttcgc gccgcatttc ttgccgcgct ttgcccgatc
     7801 gaaggcgtac gttccgtgct ggagggattg caggtcccgt attgcgtcgc ctcgtccagc
     7861 gatctcgacc gggtgtcctt ctccctatcc ctgacaggtc tcgcgccgca tttcggcagc
     7921 cggctctata gcgcgcaaat ggtggaacgc ggcaagcccg cacccgatct cttcctttac
     7981 gcggccgaga gaatgcaggc ccatccgtgc aatacccttg tgatcgagga tagcgtcagc
     8041 ggtgtaacgg cagccaaggc ggccggcatg acagtctggg gatttgtcgg aggcagccac
     8101 tatcaatcgc gtgacggcaa ggccatcctc ctggacgcgg gagccgaccg cgtttttgaa
     8161 cgaatggcgg atttttggcc gaatgaatcg gaagaatttt atggtcgcgc ctgatagcga
     8221 aaaatcgcgc ctcgacgaag cggctcgcgc cggctggctg tatttcatcg ccggtcatac
     8281 ccaggacgag atcgccaaga tgctccaggt ctcgcgcgcc tcggcgcagc gcctcgtctc
     8341 attgtgcctt gccgaacgcc tgatcacctt ccgtctggag catccgatcg ccgcatgcat
     8401 ggagttagcg gcgcgtctca aggacttgtt tcacctcgtc tattgcgaag tggtgccgaa
     8461 tgatcccgcc gcgcaacttt cggccaccgg aattgccgaa cgggctgcaa acatcctgga
     8521 atcgacgttg cgcaccgaga aacctgctat tgtggcgctg gggaccggga gagccgtgcg
     8581 cgccgcggtg gagcgggtct cgccgaccga ttgtcccaac caccagatcg tgtcgctggt
     8641 cggaaacatc tcggccgacg gctcggcgag ctttttcgac accgtggggc gtctcgcgga
     8701 ccggaccaag gcgcggcatt atcccatgcc gctgccgttc ctgatgtcgt ccgagcacga
     8761 gcgcgaccag atgctccggc tcgacccgat cgccagggtt cgcgccgtcg cagcgaaggc
     8821 tgacttgcgg cttgtcggga tcgggcagat ggatcagcgc gcgcaagtgt tcgtcgatgg
     8881 cttcgtctcg cgcgaagaac tgctggagat gatgcggctg ggcgcggttg gcgaactgac
     8941 cggctgggcg ttcgacgatc agggccacat catcgatggc gggacaaacc gccgtctgac
     9001 cagcattcca ccacgggtcc ctgccgaggc cctgaccatc ggcgcggcgg tcggccgcgc
     9061 caaggtttcg gcgatcaggg ccgcgctaaa ggggcggttg ctcaacgggt tgatcaccga
     9121 cgaagcgacc gcaggcgcca tcctcaagcc gtagcgaatg actggccttg caagccgcgt
     9181 tgggcaggcg aacggccaga agccgatccg ggcgcgtgcg acccgttcga accatctcca
     9241 tacctcaatt tcggtgcaga agccggattc cgatccttga caaggccggc actcggtgtg
     9301 aacataattg caagacgtgg gcagatgctc aacgtcaaaa tagggaggtt accgtgaagc
     9361 acatcctcgg cgccctgctg ggcgcggtta ccctgttggg ctcgggcccc tcgattgcac
     9421 agacaacgct gacgatcgcc accgtgaaca acggcgacat gatccgcatg caggcgttga
     9481 cgaacgagtt cactgccaaa aatcccgacg tgaccgtgaa atgggtgacg ctggaagaga
     9541 acgtcctgcg ccagcgcgtc acgaccgaca tcgcgaccaa gggcggtcag ttcgacgtgc
     9601 tcacgatcgg cacctacgag gtcccgatct gggccaagaa gaactggctc attccgctcg
     9661 acaatctcgg agccgactac gacgccgcgg atctgcttcc gaggatcagc
//
LOCUS       DATNOI010000512         7579 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1322883,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000512 DATNOI010000000
VERSION     DATNOI010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322883"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /gene="zapE"
                     /locus_tag="VN968_20600"
     CDS             complement(<1..920)
                     /gene="zapE"
                     /locus_tag="VN968_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HXL31670.1"
                     /translation="MLSTPSVSFSAHYQALVASGAIEPDAAQAEAADAFAGLERRLAG
                     YRPLRKQGLLGRLFADKDVAPPRGLYVHGEVGRGKTMLMDLFFQHSQVVHKRRAHFHE
                     FMAEVHERIYGYRQNIARGEIADGDVIALTAGSIFDEAWLLCFDEFHVTDIADAMILG
                     RLFSKLFELGTVVVATSNVAPEDLYKGGLNRALFLPFIAQISDHMDMLRLDARTDFRL
                     EKLAGVKMWLVPADRAADAALDQAWARMTGKAPCKPRDISIKGRVLHVPCSADGVARF
                     SFAEICEKPLAASDYLRLAHDYHTLLIDRIP"
     gene            complement(1157..1693)
                     /gene="thpR"
                     /locus_tag="VN968_20605"
     CDS             complement(1157..1693)
                     /gene="thpR"
                     /locus_tag="VN968_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HXL31671.1"
                     /translation="MPRLFTGLEIPAEIGQTLSDLRGGLPGARWIDPENYHVTLRFIG
                     DIDGVAANEIASLLFRVNRKPFEVALQGLSSFGGRKPRAVVASIAPSRPLIELQAELE
                     RLMQRFGLDPEGRKFTPHVTLARLRDASNQDVADYLSVRGYFPTRVFTASRFVLFSSR
                     ASTGGGPYVVEDSYALSA"
     gene            complement(1893..2540)
                     /locus_tag="VN968_20610"
     CDS             complement(1893..2540)
                     /locus_tag="VN968_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HXL31672.1"
                     /translation="MHILVLAIVFMTAGIASAQTSATGPTKPIKMVVLGDSLSAGLGL
                     SAASAFPVRLQKSLKINGIDVDMINAGVSGDTSSGGRDRLDWSVPPGTDAVILELGAN
                     DALRGTDPKVTRAALTDILTQLKARGVAVLLCGMVAPPNYGSDYAARFNAIYPDLAKS
                     FGVPLYPFFLEGVAADARLNQADGMHPTAEGVDVIVKNILPTVQAFVGAISGQRS"
     gene            2596..3330
                     /locus_tag="VN968_20615"
     CDS             2596..3330
                     /locus_tag="VN968_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31673.1"
                     /translation="MDSLIEPSSLAGPEPDTISISNVNLSLGAGAARVHILKDISLRV
                     APGEAIGLIGPSGSGKSTLLMVMAGLERPDSGEVVVNGTPFNALDEDALARFRGRQVG
                     IVFQSFHLIPTMTALENVAVPLELAGNPDASQRAAQELTSVGLGDRLHHYPTQLSGGE
                     QQRVALARALAPDPAILVADEPTGNLDEATGKQIVDLLFTKHAERGMTLVLVTHDTSL
                     AQRCDRVVRLRSGRIDGHVAERETAS"
     gene            3327..5912
                     /locus_tag="VN968_20620"
     CDS             3327..5912
                     /locus_tag="VN968_20620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXL31674.1"
                     /translation="MTIASEAIASEPITGGRAAPLALRYALRELRGGLRGFYVFIGCI
                     ALGVMAIAGVGSVAASLSEGLAREGRTLLGGDLAFSLIQREARPQEVAFLRSRGQVSV
                     VATLRGMARAADGRIALVELKAVDAAYPMLGELTLDPKMPVADLLTERDGAFGAAADQ
                     TLLARLDLKIGDRITIGSATFQIRSVVGAEPDKLAGGLGFGPRFLVSEAALRATGLLQ
                     PGSLVRWIYRVRLPDGAADDRAATALADDARAALPEAGWEIRSRGNASPQLERTISRF
                     TQFLTLVGLAALLVGGVGVANAVKSHIDRRRDVIAAFKALGATGRDVFTIYLTQVVVL
                     AVIGSLIGLAAGAALPFVIVGVFGKLLPLPVVPALHPDELALSFVYGLLTALTFGLWP
                     LGRVHDVPVAALFREAVTSEWHRPRWRYLALMAMVVALLIAVVIGLAYDKRIAAVFVA
                     SSVVVFALLRGIAAALMALARRLPRTRFTMLRLAIANIYRPGALTPSVVMSLGLGLAV
                     LVTITQIDGNLRRQFLAALPDRAPSFYFIDIPTADAGRFGAFLKQTAPQSTVEDVPML
                     RGRIVAARGVKAEDLKASTDAEWVLQSDRGLTYTGEIPKGSKIVEGQWWGTDYDGPPL
                     VSMEKKIADGLNLKIGDEIVVNVLGRDIPATIGNLRTVDWQSLGINFVLVFSPNAFKG
                     APHSHIATLTENHPDPADDARIIRSVADAFPTVTSVRVREALESIGTVITNLVLAIRG
                     ASAVTLISAILVLGGALAAGHRHRVYDAVILKTLGATRARLLGAYALEYLMIGFATAI
                     FGVIAGSVAAWLIVTRLMTLSFIWQAGSAAGVVAAALVVTVGLGLAGTLLALNQKPAT
                     VLRNL"
     gene            6172..6963
                     /locus_tag="VN968_20625"
     CDS             6172..6963
                     /locus_tag="VN968_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HXL31675.1"
                     /translation="MSDLDRNYASPFGRAAGRVDAATVDAGLRAYMLRIYNYMSIGLA
                     ITGLAALGVYMAAVTADPAGDAVRVGKIYLTPFGYAMFVSPLKWLFMLAPLAMVFVIS
                     AGIDRLKPATAQMLFWVFSALMGISLSSIFLVYTHTSIVRVFFITAATFGALSLYGYT
                     TKRDMSGMGSFLMMGLFGIIIASLVNLFLASSPLQFFISVIGVLIFAGLTAYDTQRLK
                     NDYIYGYASQGGAVAERAAITGALSLYLNFINLFTLLLQLLGQRD"
     gene            7048..7572
                     /locus_tag="VN968_20630"
     CDS             7048..7572
                     /locus_tag="VN968_20630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL31676.1"
                     /translation="MTSPEIRAAAAADLPAITEIYEHAVRYGTATFELIPPDLTEMTR
                     RFGVLLDGGFPYLVAALEGRVVGYAYAGAYRPRPAYRFTVENSVYLQPAIHRRGIGLQ
                     LLQRLITESEQRGYRQMIAVVGDSANAGSIGVHTKCGFQMIGTHPDVGFKFGRWLDTV
                     MMQRALGDGGSTLP"
BASE COUNT         1332 a         2450 c         2383 g         1414 t
ORIGIN      
        1 ggaatgcggt cgatcaggag ggtgtgatag tcgtgcgcga gtcgcagata gtcggatgcg
       61 gcaagcggct tttcgcagat ctccgcgaac gaaaatcgcg caacgccatc cgccgaacac
      121 ggcacgtgca ggacacggcc cttgatcgag atatcgcgag gcttgcaggg cgccttgccg
      181 gtcatccgcg cccaggcctg gtcgagcgcg gcatcggcgg cgcgatccgc cggcaccagc
      241 cacattttca cgcccgcgag cttctccagc cgaaaatcgg tgcgcgcgtc gagccgcagc
      301 atgtccatgt gatcggatat ctgggcgatg aagggcagaa acagcgcccg gttcagaccg
      361 cccttgtaga gatcctcggg cgcgacgttc gaggtcgcca ccaccaccgt gccgagttcg
      421 aacagttttg agaacaaccg tcccaggatc atcgcgtccg cgatgtcggt gacatgaaat
      481 tcatcgaaac acaaaagcca ggcctcgtcg aagatcgacc cggcggtgag cgcgatcaca
      541 tcgccatcgg cgatttcgcc acgcgcgata ttctgacgat agccatagat ccgctcgtgc
      601 acttcggcca tgaattcgtg aaaatgcgcg cggcgcttgt gcaccacctg gctgtgctgg
      661 aaaaacagat ccatcagcat ggtcttgccg cgcccgactt cgccatgaac atagaggccg
      721 cgcggcggcg cgacgtcctt gtcggcaaac agccggccca gcaggccctg cttgcgcaag
      781 ggcctgtagc ccgcgagccg ccgctcgagg cccgcgaagg catcggcggc ctccgcttgc
      841 gcggcgtcgg gctcgatcgc gccagaggcg acgagcgcct gataatgggc gctgaacgag
      901 acggatggag tggacagcag catggctcct cttacggccc caacattaag gaaattgcaa
      961 gctgtgaatt gaggctgagg gtatgcaggc gcgggcgtca gggggcaccg cgccgaccca
     1021 tctcaagccg tcataccggc gaaaagcgga tatttggtac gcagcggctt tcgcttccat
     1081 caccggcggc tctggaatac ttggatcatc cgctttcgcg gatgatgacg gctgtgaatg
     1141 cggcgagcgt ctgccctcac gcgctcagcg cgtaggaatc ctcgaccaca tagggcccgc
     1201 cgccggtgga ggctcgggac gagaacagca cgaaccgcga tgcggtaaac accctggtcg
     1261 gaaaatatcc gcgcaccgac agatagtccg cgacatcctg gttggaggcg tcgcgcagcc
     1321 gcgccagcgt cacatgcggg gtgaatttcc gcccttcggg atcgagaccg aaacgctgca
     1381 tcagccgctc caactcggcc tgcagctcga tcagcgggcg gctcggcgcg atcgacgcga
     1441 ccaccgcgcg cggcttgcgc ccgccgaaac tcgacaggcc ctgcagggca acctcgaacg
     1501 gctttcgatt gacccggaac agcagcgagg cgatttcgtt ggccgcgacg ccgtcgatat
     1561 cgccgatgaa gcgcaaggtg acgtgataat tttcgggatc gatccagcgc gcgcccggca
     1621 agccgccgcg caaatcggaa agcgtctggc cgatctcggc cggaatttcc agtccagtga
     1681 acaagcgtgg catcgcatga ctcccgatgc tagggcctga tcccgaaaat tagacaccgg
     1741 ttttcggaaa gatcatgccc tgaagtaaaa cccggtgacg aatcaccgat gctgattttt
     1801 accgtagtta tatgaccgtg cgaagcatcc gcagttcaag cacgacaaag cttccgggaa
     1861 aacatcgcat tagggttatc actgcctgtt tttcaactgc gttgcccgga tatcgcaccg
     1921 acaaatgcct gcacggtagg caggatattc ttaacgatta catcgacacc ctccgcggtc
     1981 ggatgcatgc catccgcctg attgaggcgg gcgtccgcag cgacgccctc caggaaaaac
     2041 ggatacaacg gcaccccgaa cgatttcgca agatccggat agatcgcgtt gaaacgcgct
     2101 gcatagtcgc tgccgtaatt cggcggcgca accatcccgc acaacaaaac cgccactccg
     2161 cgcgccttca gctgcgtcag aatgtcggtc aatgcggcgc gggtaacctt gggatcggtg
     2221 ccgcgcaagg catcgttggc gccgagttcg aggatcacgg cgtcggttcc cgggggcacc
     2281 gaccagtcga gccggtcgcg accaccggag ctggtgtcgc cggacacccc ggcattgatc
     2341 atgtcgacat ctatcccatt gatctttaaa gatttttgga gccgaaccgg aaacgctgac
     2401 gctgccgaca ggccaaggcc cgcgctcagc gagtcaccca atacaaccat cttgatcggt
     2461 tttgtcggcc cggttgccga cgtttgcgcc gaggctattc ccgccgtcat gaaaacgatg
     2521 gccaacacaa gtatgtgcat gaacatccct cgccgcccct cgaccccagc ggcgaaagtg
     2581 ccatatgacc gagccatgga cagtctcatc gaaccctcgt cattggccgg ccctgagccg
     2641 gacaccattt ccatctcgaa cgtcaacctc tcgcttggcg cgggcgcggc gcgcgttcat
     2701 atcctcaaag acatcagcct gcgtgtggca ccgggtgaag cgatcggctt gatcggacct
     2761 tccggctccg gaaaatccac tctgctgatg gtgatggcgg gactggagcg tcctgacagc
     2821 ggagaagtgg tggtcaacgg cacccctttc aatgccctcg acgaagacgc cctggcgcgc
     2881 tttcgcggcc gccaggtcgg gatcgtattc caatcgttcc atctgattcc gaccatgacg
     2941 gcactggaaa atgtcgcggt gccgctggaa ctcgccggca atcccgatgc gtcgcaacgc
     3001 gcggcgcagg aactgacatc ggtcggcctc ggcgaccgcc tgcatcacta tcctacccaa
     3061 ttgtccggcg gcgagcagca gcgcgtcgcg ctggcccggg cgcttgcgcc cgatccggcg
     3121 atcctggtcg cggacgaacc caccggcaat ctggatgaag ccaccggcaa gcagatcgtc
     3181 gatctgttgt tcaccaaaca cgccgagcgc ggcatgaccc tggtgctggt gacccacgac
     3241 acctcgctgg cgcagcgctg cgatcgcgtg gtgcggctgc gctcgggccg gatcgatggg
     3301 catgttgcgg aacgcgaaac cgcttcatga ccattgcctc cgaagccatt gcctccgaac
     3361 cgatcactgg cggccgcgcg gcaccgctgg ccctgcgcta tgccttgcgc gaattgcgcg
     3421 gcggcttgcg cggtttttac gtcttcatcg gctgtatcgc gctcggcgtg atggcgatcg
     3481 ccggcgtcgg ttcggtcgcg gcaagcctca gcgaaggact ggcgcgcgag ggccgcaccc
     3541 tgctcggcgg cgacctggcg ttctcgttga tccagcgcga ggccaggccg caagaagtcg
     3601 cgtttcttcg ttcacgcgga caggtatctg tcgtggcaac cttgcgcggc atggcgcgcg
     3661 cagccgacgg ccgaatagca ttggtcgaac tgaaggcggt ggacgccgcc tatccgatgc
     3721 tgggcgaact cacgctcgac ccgaaaatgc cggtcgccga tctgctgacg gagcgcgacg
     3781 gcgcgttcgg cgcggctgcc gaccagacct tgctggcgcg gctcgatctc aagatcggcg
     3841 accgtatcac gatcggcagc gcgacttttc aaatccgcag cgtcgtcggc gccgagccgg
     3901 acaagctggc cggcgggctc gggtttggtc cgcgattcct ggtcagcgaa gccgccctgc
     3961 gcgcgaccgg attgttgcag cctggcagcc tggtgcgctg gatttatcgt gtcaggctgc
     4021 cggacggcgc ggccgacgac cgtgccgcga ccgcgctggc ggatgatgcg agagccgcat
     4081 tgcctgaagc aggctgggag attcgcagcc gcggcaatgc ttcgccgcaa ctggagcgca
     4141 ccatcagccg cttcacgcag tttctcacgc tggttggcct cgcggccctg ctggtcggag
     4201 gcgtcggcgt cgccaacgcg gtcaaaagcc atatcgatcg ccgccgcgac gtcatcgcgg
     4261 cgttcaaggc gctgggcgcc accggccgcg acgttttcac gatctatctg acccaggtgg
     4321 tcgtgctcgc cgtgatcggc tcgctgatcg ggctggccgc cggcgcggca ctgcccttcg
     4381 tcatcgtcgg cgtgttcggc aagctgctgc cgctgccggt agtgccggcg ctgcatccgg
     4441 acgaactcgc tttatcgttc gtctacggcc tcctgaccgc gctcaccttc ggactttggc
     4501 cgctcggccg ggtgcatgac gtgccggtgg cggcgctgtt tcgcgaagcg gtgacgagcg
     4561 agtggcatcg cccgcgctgg cgctatctcg cgttgatggc catggtggtc gcattgctga
     4621 tcgcagtggt gatcgggctc gcctacgaca agcgtatcgc ggcggtgttc gtcgcatcct
     4681 cggttgtggt atttgcgctg ctgcgcggca tcgccgccgc cctgatggcg ctggcgcgcc
     4741 gactgccgcg gacccgcttc accatgctgc ggctggcgat cgccaacatc taccggcccg
     4801 gcgcgctgac gccgtcggtg gtgatgtcgc tgggcctcgg cctggcggtg ctggtcacga
     4861 tcacccagat cgacggcaat ctgcggcggc aattcctggc agcactgccg gaccgcgcgc
     4921 cgtcgttcta tttcatcgat attcccaccg ccgacgccgg ccgcttcggc gcattcctca
     4981 agcaaaccgc gccgcaatcg accgtcgagg acgtgccgat gctgcgcgga cgcatcgtcg
     5041 ccgcccgcgg tgtcaaggcg gaagacctca aggcctcgac cgacgccgaa tgggtgctgc
     5101 agagcgaccg cggcctgacc tataccggcg aaatcccgaa gggttcgaaa atcgtcgaag
     5161 gccaatggtg gggaaccgac tatgacggtc cgccgctggt ctcgatggag aagaagatcg
     5221 ccgacggtct caatctcaag atcggcgatg agatcgtcgt caacgtgctc ggccgcgata
     5281 ttccggccac gatcggcaat ttgcgcaccg tggactggca gagcctcggc atcaatttcg
     5341 tgctggtgtt ctcccccaat gccttcaagg gcgcccccca tagccatatc gcgacgctga
     5401 ccgagaacca cccggatccc gccgacgacg cccgtatcat cagatcggtg gccgacgcat
     5461 tcccgacggt gacaagcgtg cgggttcgcg aggcgctcga aagcatcggc accgtgatca
     5521 caaacctggt gctggcgatc cggggcgcca gcgcggtcac cctgatctcg gccatcctgg
     5581 tgctgggcgg ggcgctcgcc gccggccatc gccatcgggt ctacgacgcg gtcattctca
     5641 agaccctggg cgccacccgg gcgcggctgc tcggcgccta cgcgctggaa tacctgatga
     5701 tcggcttcgc cacggcgatt ttcggcgtga tcgcgggctc ggtggcggcc tggctgatcg
     5761 tgacccggct gatgacgctg agcttcatct ggcaggccgg cagcgctgcg ggcgtggtgg
     5821 cggcggcgct ggtcgtcacg gtggggctgg ggctcgccgg cacccttttg gccctgaacc
     5881 agaagccggc cacggtgctg cggaatttat gataaattga agcaggctac gcgggggttg
     5941 acccggtccc gctttccggc gcctgcccgg tgaatcatgc cgcggagcga cattcagccg
     6001 cgcatggaag cttgcggtga atgtgctagt ttcccacata tccgctcggg atcagatgcc
     6061 ggtttgatga cgcaaaatta atgtcagcaa atttcggtat ctgggatagt tttcttagat
     6121 tggcgccgca aaccccttgc gctccgcctg acaaacaacg ggaattcgac catgtcggac
     6181 ctagaccgca attatgcttc tcccttcggc cgggccgccg ggcgcgttga cgccgcgacc
     6241 gtcgacgctg gtctgcgcgc ctacatgctg cgcatctaca attacatgag cattggcctg
     6301 gccatcaccg gcctcgccgc gctcggcgtc tatatggcgg cagtgacagc ggacccggcg
     6361 ggcgacgccg tccgggtcgg taagatctat ctgacaccgt tcggctacgc gatgttcgtc
     6421 agcccgctga aatggctgtt catgctggcg ccgctggcga tggtgttcgt gatctcggcc
     6481 ggcatcgatc ggctcaagcc cgcgacggcc cagatgctgt tctgggtatt ctcggcgctg
     6541 atgggcattt cgctgtcgtc gatcttcctg gtgtatacgc acacctcgat cgtccgggtg
     6601 ttcttcatca ccgcggccac gttcggcgcg ctgagcctct acggctacac caccaaacgt
     6661 gacatgagcg ggatgggatc gttcctgatg atgggcctgt tcggcatcat catcgcgagc
     6721 ctggtcaatc tgttcctggc cagttcgccg ctgcagttct ttatctcggt gattggcgtg
     6781 ctgatctttg cgggcctcac cgcctacgat acccagcggc tgaagaacga ctacatctac
     6841 ggctatgcct cgcagggcgg tgccgtcgcc gagcgggcgg cgatcaccgg cgcgctgtcg
     6901 ctgtatttga acttcatcaa cctgttcacg ctgctgctgc agctgctcgg ccagcgcgac
     6961 tgaacgcttt cgcggtagcg aaaattcaag ccccggcctt gcggccgggg cttttctttt
     7021 ggcgcggacg aaaataatct tcgcgccatg acctctcccg aaatcagggc cgccgccgcg
     7081 gccgatctcc ctgccattac cgagatctac gagcacgcgg tgcgctacgg aaccgcgacg
     7141 ttcgaactga tcccgccgga tctcaccgag atgacgcggc gctttggcgt attgctggac
     7201 ggcggctttc cctatctggt ggcagccctt gagggccgcg tggtcggata cgcctatgcc
     7261 ggcgcgtacc ggccgcggcc ggcctaccgc ttcacggtgg agaattcggt ctatctgcag
     7321 cctgcgatcc accggcgcgg gatcggtctg caactgctgc aacggctgat caccgagtcg
     7381 gaacagcgcg gctatcgcca gatgatcgcg gtggtcggcg attccgccaa cgccggatcg
     7441 atcggcgtgc acacgaaatg cggatttcag atgatcggga cccatcctga tgtcggcttc
     7501 aaattcggcc gctggctcga caccgtgatg atgcagcgcg cgctcggcga cggcggctcg
     7561 acgctgccct agactctgt
//
LOCUS       DATNOI010000513        13494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1048600,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000513 DATNOI010000000
VERSION     DATNOI010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13494
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048600"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VN968_20635"
     CDS             complement(<1..484)
                     /locus_tag="VN968_20635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HXL31677.1"
                     /translation="MSEAVVDDIVVVLPPLLQSLEALGFIARHLNPPQFDAVMEAAGT
                     PDQALQAVRPRLVDRPDKFAHLRAPLVTASDAALAAFEGLRAVQHGNGDLVSVFRALR
                     YAPRAQEALYPLTAKLPPVSNFFIDPPLREDADLLARLAEPGQENTGIMHHDNEPGSR
                     G"
     gene            complement(499..1470)
                     /locus_tag="VN968_20640"
     CDS             complement(499..1470)
                     /locus_tag="VN968_20640"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HXL31678.1"
                     /translation="MASLTEWKVPLAAQPRAGDYGFDLDRVLASVVGLHSIIPADAFS
                     ADTLGTERAGNGVLIDDGLVLTIGYLITEAEAVWLHLGDGRVVEGHALGFDSVSGFGL
                     VQALGRIDLDPVPLGCSADAKIGDRVVVGGAGGRTRSVASHIAAKQEFAGYWEYLLDE
                     AIFTYPAHPNWGGTGLISNRGELIGIGSLQLERERDGKAEHVNMTVPIDLLKPVLDDL
                     RKFGRVNKPARPWLGMLSTEIENRVVVVGISAKGPAARAELKAGDVILAVNGEKISSQ
                     TQFYRKLWSLGPAGVDVPLTVYHEGVTFDVVLASTDRIKLLKAPRLH"
     gene            complement(1538..2020)
                     /locus_tag="VN968_20645"
     CDS             complement(1538..2020)
                     /locus_tag="VN968_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HXL31679.1"
                     /translation="MSFSKDNSAYEAWLAKQCDVVAKDVDYKHHRMKKSAFIFLRATY
                     FRWAKKIGTLCPELMDAPQVLSVGDLHLENFGTWRDADGRLVWGVNDFDEAAVMPYPL
                     DLVRLAASIRLAPGGSISNQATAVAAVKRDFEEWRNCRTSDLDPRSFHCYKSHKTVMP
                     "
     gene            2346..3764
                     /locus_tag="VN968_20650"
     CDS             2346..3764
                     /locus_tag="VN968_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXL31680.1"
                     /translation="MDNRNDIWLGVDAIKPRFIALSDKVWAMPEVCYTEARSSAEHLA
                     ELRHQGFRIAENVAGIPTAVMGEWGEGGPVIAFLGEYDALPGLSQEADVAEPRPLEVG
                     GHGHGCGHNLLGSAALLAATAVKDWLVAHKMPGRVRYYGCPAEEGGAAKTFMVRSGAF
                     DDADIAITWHPSSFWEVVVTPSLANTRADFIFTGRASHAAASPHLGRSALDAVELMNV
                     GVNYMREHMPSDARVHYALLDAGGIAPNVVQAHARVRYSIRARDLPGMNELVERVHKI
                     AQGAALMTETRMEMRIISAVSNVLPNTPLEQTLHRIMEDLGPPRFDDADKEFAAKIRS
                     TLTEKDIASVYHAIGMDPTDRPLADFIVPLDARRNPLIGSTDVGDVSWVVPTVQVHAP
                     TVAIGTPFHTWQIVAQGKSPAAHKAMVQAAKAMAALGVKALNEPDLIAAAKADLTKRT
                     ARTPYVSPLPDNVAPPLDMSLS"
     gene            3816..4685
                     /locus_tag="VN968_20655"
     CDS             3816..4685
                     /locus_tag="VN968_20655"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HXL31681.1"
                     /translation="MESPPLPYVAVAESFKLPPGMNFRGTSGVAFNSKGNIFVVHRGL
                     MPVMEFDPDGNFIRGFGEGMFERSHGLRIDAQDNIWATDVAANLVYKFNPSGRLEMVL
                     GVKGHTGEWHPFGHLRLFHESNEAVVGPTGDIFVLQGHGKGESRVLKFDQDGSFIKTW
                     GGKGKGPGEFDLPHSLVFDAQGLLYIADRNNARIQVFDADGNYIRESQHPGAPCGLFM
                     GADQHIWLAHGHTGQIMKLDLNGRVVGMMAGAGQGKTLGKYGEAHYIAVSSRDEVFVA
                     DTLNWRIQKYTRA"
     gene            4682..6265
                     /locus_tag="VN968_20660"
     CDS             4682..6265
                     /locus_tag="VN968_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXL31682.1"
                     /translation="MIDQEYDTIIIGGGSAGATLAARLSEDPLHRVLLLEAGQDLRTA
                     DTPEHIQIPNPMCAIGDDNFRWPKLLARRTDRQEPRLLWRGRAMGGSSTINGQIAIRA
                     VPDDLDRWEANGCPGWGWDVMLPYFCKLETDKNFPDAAYHGDRGPIPVYRAPVSDWGN
                     VDQALLQSALGLGYGWCDDHNAPTGTGVSPYAINSEAGRRVSTNDGYLEPARGRENLR
                     IVGHALVEAIEFEGNRLHARAVRVRAGGRSYAPTAKHEVILCAGAIHSPAVLQRSGIG
                     PAALLEAFGIPILADLPVGENLLDHPIMDALLHLRENGQVGTLMHRHTNCCLRYSSGL
                     EGAGENDMIMIAGNLARDKQQTVDAARGRIAVSVYQAFSQGHVRITTSDPTIDPAVEE
                     RMLSDRSDLIRMRDGVRRLREICLQPAVTDIAHRVEYGATGHAIDEALSDAELDDWLF
                     AECSDAQHASGTCRMGAAGDPRSVVDPECRVIGCTGLRVIDASIMPEVPRANTHLTTV
                     AIAERMADRLKAAKRGRPA"
     gene            complement(6317..7219)
                     /locus_tag="VN968_20665"
     CDS             complement(6317..7219)
                     /locus_tag="VN968_20665"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL31683.1"
                     /translation="MNLSAVDMNLLVLLEALLDEAHVRRAGERVGLSQPAASHALARL
                     RDLFDDPLLVRVGTAMVRTPRAEALRKPLAQFVASAQAVLRMDDFNPHTSKRAFRFML
                     PDLVSHLIMPSLVARVARTAPGIRVKLIPWRGPALLTDRTLAEIDLFVTSFNREFSGF
                     ARSPLYEDKDILVVRAGHPGKRALSTVKGFQASQHVSVVGAGEDRDELDEWLDKVRLQ
                     RNVAVAAPSHLIALRIAAATDYVAFVPRRFAAARKAELDLALIKPPIDPGIDILYLMT
                     PTKSASDKGALWMKSQVEDIARTL"
     gene            7263..7700
                     /locus_tag="VN968_20670"
     CDS             7263..7700
                     /locus_tag="VN968_20670"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXL31684.1"
                     /translation="MGSYRKLRMSEWLRANPPGLPVTPGSSQIAEARGRLIFISGQVA
                     VTADDEHVGAGDFGVQLEQVFRNLDTAVRAAGGRFEDITKLNYFCLDSVDRALLPEVR
                     RVRDSYVNAECPPASTFVFVSGLARKGWLIEIEAVATLRDQAQ"
     gene            7697..8410
                     /locus_tag="VN968_20675"
     CDS             7697..8410
                     /locus_tag="VN968_20675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL31685.1"
                     /translation="MSQRPDLVLVTGPMVGASSWAPTASHLRAAGWCVHVPDILSANG
                     ALPPWHALSAHYAKLLSLDNPPILVGHSLATVVVADLAVCVSARGLIMVDGEIPPASG
                     PVAPGRESFRAFVLGLAGDDGWLPRWSEWWLDHPSRSVTGIDQLAHDADAFALFANDQ
                     PRVPAAWFDDTIDLAPWAQSPAGYIQTSRFYDHSAEDAEARGWPLLRLQGTHLHPTLQ
                     PDETALAIAEIARKLVHGS"
     gene            complement(8481..9038)
                     /locus_tag="VN968_20680"
     CDS             complement(8481..9038)
                     /locus_tag="VN968_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXL31686.1"
                     /translation="MNAHVSQGGWPVLVLNADFRPLSYYPLSLWSWQDAIKAVFLDRV
                     NIVEHYDRAVRSPSFEIQLPSVVSLKSFVKPTTHPAFTRFNVFLRDRFVCQYCSAHDD
                     LTFDHIIPRSKGGQTTWENVVAACSPCNLRKGNLTPQQARMFPRQFPFAPTVHQLHRN
                     GRLFPPNYLHDSWLDYLYWDTELDP"
     gene            9326..9511
                     /locus_tag="VN968_20685"
     CDS             9326..9511
                     /locus_tag="VN968_20685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31687.1"
                     /translation="MRRLLTVMVAALFGLAATGMAAAQGYPAHAMTMIVPFPAGGATD
                     TWLAFWASVCAEFWVNP"
     gene            9484..10302
                     /locus_tag="VN968_20690"
     CDS             9484..10302
                     /locus_tag="VN968_20690"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL31688.1"
                     /translation="MRGILGQPVIIENVAGAAGSLGVGRAVRSAADGYTLSIGTSTTH
                     MLTGGLYALQFDLLKDLEPVIQIGSEPLLIVGKKSLPAADLKGLIGYLKANPDKASVG
                     IAGVGATGHLTGISFQKETGTRFQFVPYRGNAPAMQDLLAEQIDFMIEPSSNFKSLVG
                     AGSVRPYAITGRTRLPSSPDIPTADEAGLRGFFASLWYGLWVPKDTPKDIIAKLNATM
                     VQVLADPMVRKRFDELGIEITPLAQQSPEALRAFQKAESERWWPIIKASNIKAE"
     gene            10506..11360
                     /locus_tag="VN968_20695"
     CDS             10506..11360
                     /locus_tag="VN968_20695"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HXL31689.1"
                     /translation="MKVHAIQTGFVRIKTAQVEGQGHGLPRRLAIFADRNWTGWLPTY
                     AWIIDHPEGIIVVDTGQGAHLLESGKSLHPYVRWEVGFRIDREQEIGPQLKALGIGPR
                     DVKRVVLTHLHMDHDGGLAHFPNSEILVAPGELQTASGYVGRMRGYLPHRWPSWFDPK
                     PLNLTAESSGPFAMSRRLTKAGDVIAVATPGHTADHISIIVQASEITYFLAGDTSYNE
                     ELMLAGKLDGVSANEQVTGATLAAIRRFTQSRPTVYLPTHDPGAAERLANRGLAAVRE
                     GLLSNAPA"
     gene            complement(11326..12192)
                     /locus_tag="VN968_20700"
     CDS             complement(11326..12192)
                     /locus_tag="VN968_20700"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXL31690.1"
                     /translation="MQATLSRRSSDGLEGLCEHTPGGARETARISMGLGCDGIERLEA
                     RFRGQAFSPHRHDTYAIGITLAGVQTFRYRGEARHCLPGQCHILHPDEIHDGASGTSD
                     GFAYRILYIDPCLVQGAVGGRPLPFVSNPIIDLTNRQRDALSAAWEMEVPIDDIGRIE
                     IVTAVADILLSACSDAARPLSALPLPGLLRARARIAARPADRHPINELERVADLDRWS
                     LARQFRAAFGTSPSRFRTMRQLDQVRRLVKSGSSLADAALDAGFADQSHMSRQFKRAY
                     GLTPARWRAALL"
     gene            12217..12837
                     /locus_tag="VN968_20705"
     CDS             12217..12837
                     /locus_tag="VN968_20705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015338902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HXL31691.1"
                     /translation="MTGHVFSSPWQSSLTLVLEAAAIMGTPGPSTMAVMATAAAFGGR
                     RALGFVSGAIAGTIAVLLAVATGVVALLVSIQGLGFVLTLASAVYILYLALKIATAPP
                     LPKQTAEAAAPSLAAGFLLAIANPKAWFAIAAVFTGSTLLESSRELDALLKAAVLAVM
                     IVLIHLCWLLGGVSLAGFLRDPLRSRIVNVLFGLVLVGTTALALIR"
     gene            complement(12841..>13494)
                     /locus_tag="VN968_20710"
     CDS             complement(12841..>13494)
                     /locus_tag="VN968_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HXL31692.1"
                     /translation="LLTIGIPNKPAPFMSGEPFHRCTHTALSPKGEIYVSDGYGNACV
                     HKYTPDGKLLKTWGEPGTDPGQFNIVHNIVTDQDGWVYVADRENHRVQVFDGNGKYEA
                     QWNNLHRPCALHCCGGKQPNFIIGELGPGMPVNLKVPNLGPRLSIVDSKGKRIARLGG
                     ENGPGLETGKFLAPHGIALDSKGDIYVGEVGVTNWKTSFPDTPMPAVVRCLQKLERI"
BASE COUNT         2489 a         4226 c         4283 g         2496 t
ORIGIN      
        1 cgccgcggct gccgggttcg ttgtcgtggt gcatgatccc ggtattttcc tgtcccggct
       61 cggcgagacg cgccagcaga tcggcgtctt cgcgcagagg aggatcgatg aagaaattgc
      121 tcaccggcgg cagttttgcg gtgagcggat acagcgcctc ctgcgcgcgg ggagcgtaac
      181 gcagcgcgcg aaacacggag accaggtcgc cgttgccgtg ctgtaccgcg cgaagtccct
      241 cgaacgccgc aagtgccgca tcgcttgcgg tcacaagcgg cgcgcgcaga tgcgcgaact
      301 tgtccggccg atcaaccagc cgcggacgga cggcctgcaa cgcctgatcc ggcgtgccgg
      361 cggcttccat gacagcatcg aattgcggcg ggttcagatg acgggcgatg aaaccgaggg
      421 cttcgagcga ttgcagcaac ggcggcaaca ccaccacgat gtcgtccacc acggcctcgc
      481 tcatcgcgcg cccctgtgtc aatgcagccg cggcgccttg agcagtttga tccggtcggt
      541 tgaggccagg accacgtcga acgtgacgcc ttcgtggtag acggtgagcg gcacatcgac
      601 acctgcgggt ccgagcgacc agagttttcg gtagaactgc gtttggcttg agatcttttc
      661 gccgttgacg gcgaggatga cgtcgccggc cttgagttcg gcgcgcgccg ccggcccctt
      721 cgcggagatg ccaaccacca ccaccctgtt ctcgatctcg gttgaaagca tgccgagcca
      781 gggccgcgcc ggtttattga cgcggccgaa ttttcgcagg tcgtcgagca ccggcttgag
      841 cagatcgatc ggcacggtca tgttgacatg ctcggccttg ccgtcgcgct cgcgttcgag
      901 ctggagcgag ccgatgccga tcagctcgcc gcggttcgag atcagcccgg taccgcccca
      961 attgggatgc gcgggatagg tgaagatcgc ctcgtccagc agatactccc agtaaccggc
     1021 gaactcctgc ttggcggcga tgtggctggc gacggagcgc gtgcgtccgc ctgcgccgcc
     1081 caccaccacg cggtcaccga tcttggcatc agccgaacaa ccgagcggca ccggatcgag
     1141 atcgatgcgg ccaagcgcct gcaccaggcc gaagccgctc acggaatcaa agccgagcgc
     1201 gtgaccttcg accacgcggc cgtcgccgag atgcagccag accgcttcgg cctcggtgat
     1261 cagatagccg atggtcagca ccagcccgtc gtcgatcaag acgccgttgc cggcgcgctc
     1321 ggtacccagg gtgtccgcgc tgaaggcgtc ggcggggatg atcgaatgca ggccgaccac
     1381 cgacgcaagc acgcgatcga gatcgaaacc gtagtcgccg gcgcggggct gcgccgccag
     1441 cggcactttc cattcagtca atgaggccat gagggtctcc tgctcgacga ttgcgccggt
     1501 gcgctcagcg cgccggcatg atcggaatcc ataatggtca aggcatgacc gtcttatgag
     1561 acttataaca gtgaaacgat cgcggatcga gatcggaagt tcggcagttc cgccactcct
     1621 cgaagtcccg cttgaccgcc gccactgcgg tcgcctgatt gctgatcgaa ccgccggggg
     1681 caaggcgaat gctggctgcg agacggacta gatcgagcgg gtacggcatg acggcggctt
     1741 cgtcgaaatc gttgacgccc cagaccagac ggccgtccgc gtcgcgccag gtcccgaaat
     1801 tttccagatg cagatcgccg acggaaagta cctgcggggc atccatcagt tccggacata
     1861 gcgtcccgat cttcttggcc cagcggaaat aggtcgccct caagaatata aaggcactct
     1921 tcttcatccg gtgatgcttg tagtcgacgt ctttggcgac gacgtcgcac tgcttggcga
     1981 gccaggcttc gtaagcgcta ttgtccttgc taaatgacat cgcagacctc acgcggctcg
     2041 actgattgca atctggctct ggcgtcaatc agcgtcatcc taccttagga tgtgtcgtct
     2101 tgaaacctca cggaagctgt tgttgggctc tcttcgagaa gcagcgacca gccgtcatga
     2161 gagacgacgc aggaccctcg gccatcacta ccacaaccat cgtgcacgac tcgggcgcat
     2221 cgcgccgcga gatcgtagcg acacctcctt cgcgccggcc gcatggctgg tgctccgagc
     2281 ggcgctaggc ggggtctggc gaagctgata acgatttcac tctcacaggg atcggggacg
     2341 ggaagatgga caaccgcaac gacatttggc ttggcgttga cgcgatcaag ccgcgtttca
     2401 tcgccttgag cgacaaggtc tgggcgatgc ccgaggtgtg ctacaccgag gcgcgctcct
     2461 ccgccgaaca tctggccgaa ctgcgccatc agggtttccg catcgcagag aatgtcgcag
     2521 gcattcccac agccgtgatg ggcgaatggg gcgagggtgg ccccgtcatc gccttcctcg
     2581 gcgaatacga tgctctgccc ggtctaagcc aagaggctga tgtggcggag cctcgcccgc
     2641 tcgaggtggg aggccatggc cacggctgcg gccacaatct cctgggctcg gccgcgctgc
     2701 tcgccgccac ggccgtgaag gattggcttg tcgctcacaa gatgccgggg cgcgtgcgtt
     2761 attatggctg ccccgccgag gagggcggtg ccgcgaaaac cttcatggtg cgctcaggcg
     2821 ccttcgatga cgctgacatc gccatcacct ggcacccgtc cagcttctgg gaagtggtgg
     2881 taacgccttc gctggcaaac acgcgcgccg atttcatctt caccgggcga gcctcgcatg
     2941 ctgcggcctc gccgcatctg ggccgcagcg ctctcgacgc ggtggagctg atgaatgtcg
     3001 gcgtcaacta catgcgcgag cacatgccaa gcgacgcccg cgtccactat gccttgctcg
     3061 acgccggcgg cattgctccc aatgtggtgc aggcccatgc ccgcgtgcgc tattccatcc
     3121 gcgcccgcga tctgcccggc atgaacgaat tggtggagcg cgtccacaag atcgcgcagg
     3181 gggcggcgct gatgaccgaa accaggatgg agatgcggat catttccgcc gtctccaatg
     3241 tcttgcccaa caccccgctt gagcaaaccc tccaccggat catggaggat ctcggtcccc
     3301 cgcgtttcga cgacgcagac aaggaattcg ccgcgaagat ccgctccacc ctgaccgaga
     3361 aggacatcgc ttccgtctat cacgcgatcg gcatggatcc gaccgaccgg ccgctggccg
     3421 atttcatcgt gccgctggac gccaggcgca atccgctcat cggctcgacc gatgtgggcg
     3481 atgtgagctg ggtcgtcccg accgttcagg ttcatgcccc gacggtcgcc atcggcaccc
     3541 cgttccacac ctggcagatc gtcgcgcagg gcaagagccc ggccgcccac aaggcgatgg
     3601 tgcaggccgc caaggccatg gcagcgctcg gcgtcaaggc gcttaacgag cctgacctga
     3661 tcgccgccgc caaggccgac ctcaccaagc gcaccgcccg cacgccctat gtcagcccgc
     3721 tgccggacaa tgtcgcgccg ccgctggaca tgtcgttgag ctgaagctga acctgatttg
     3781 gcgcacgaac ttttggcatg agaggcgcgg cgagaatgga atccccgcca cttccctacg
     3841 tcgcggttgc cgaatcgttc aagctgccgc cgggcatgaa ttttcgcggc acgtcggggg
     3901 ttgccttcaa ttccaaaggc aacatcttcg tcgtccatcg cgggctgatg ccggtgatgg
     3961 aattcgaccc cgacggcaat ttcatccgcg gattcggtga agggatgttc gaacggtcgc
     4021 atggtctgcg gatcgatgcg caggacaaca tctgggcgac ggacgtcgcc gcgaacctgg
     4081 tctacaaatt caatccgtcc gggcggctcg aaatggtgct gggcgtcaag ggccacaccg
     4141 gcgagtggca tccgttcggg catctgcggc tgttccacga gtcgaacgag gcggtggtcg
     4201 gtccgaccgg cgatatcttc gtgctgcagg ggcacggcaa gggcgagtcg cgcgtgctga
     4261 agttcgacca ggacggcagc ttcatcaaga cctggggcgg caagggcaag gggccgggcg
     4321 aattcgatct gccgcactcc ctcgttttcg acgcgcaggg cctcctctac atcgccgacc
     4381 gcaacaacgc ccgcatccag gtgtttgacg ccgacggcaa ttacatccgc gagtcgcagc
     4441 atcccggtgc gccttgcggc ttattcatgg gcgccgatca gcacatctgg ctggcgcacg
     4501 gtcacaccgg tcagatcatg aagctcgatc tgaacggcag ggtggtgggg atgatggctg
     4561 gagccggtca gggcaagacg ctcggcaagt atggtgaagc ccactatatc gcggtgagct
     4621 cgcgcgacga ggttttcgtc gccgacacgc tgaactggcg catacagaag tatacccgcg
     4681 catgattgat caggagtacg acacgatcat catcggcggc ggctccgccg gggccacgtt
     4741 ggccgcgcgc ctcagcgagg accccttgca tcgcgtgctt ctgctcgagg cagggcaaga
     4801 cctccgcacc gccgacacgc cggagcacat ccagatcccg aacccgatgt gcgccatcgg
     4861 cgatgacaat ttccgctggc ccaagctgct ggcaaggcga accgaccggc aggagccgcg
     4921 attgctgtgg cgcggtcgcg ccatgggagg cagttccacg atcaacggcc agatcgccat
     4981 ccgcgcggtg ccggacgatc tcgaccgctg ggaagcaaac ggctgccccg gttggggatg
     5041 ggacgtgatg ctgccgtatt tctgcaagtt agagaccgac aagaacttcc cggatgcggc
     5101 gtatcacggc gatcgcggcc cgatcccggt atatcgtgcg ccggtgtcgg attggggaaa
     5161 cgtcgaccag gcgctgctcc aatccgcgct tggcctcggc tatggctggt gcgacgacca
     5221 caacgcgccg accgggaccg gtgtctcgcc ctacgccatc aacagcgagg ccgggcgccg
     5281 cgtctccacc aatgacggct atctcgagcc ggcacgcggc cgcgagaacc tgcggatcgt
     5341 cggccatgcc ctggtcgaag cgatagagtt cgaaggcaac cggctccacg cgcgtgcggt
     5401 ccgggtgcgc gcgggcggca ggtcatacgc gcccacggcg aagcatgagg tgatcctgtg
     5461 cgcgggggct attcactctc ctgccgttct gcagcggtcg ggtatcggtc ccgcggcgtt
     5521 gctggaggcg tttggcatcc cgatccttgc cgacctgccg gtgggcgaaa acctgctcga
     5581 tcatccgatc atggatgcgc tgctgcattt gcgcgagaac ggccaggtcg gcacgctgat
     5641 gcaccggcac accaattgct gcttgcgcta cagctccggc ctcgaaggcg ccggggaaaa
     5701 cgatatgatc atgatcgccg gcaaccttgc ccgtgacaaa cagcagacgg tcgacgcggc
     5761 gcgcggccgc atcgccgtgt cggtctatca agcgtttagc cagggccacg tccgcatcac
     5821 cacgtcagac cccacgatcg acccggcggt ggaggaacgc atgctgtccg atcgcagcga
     5881 tctgattcgc atgcgcgacg gggtgcgccg cctgcgggag atatgcctgc agcccgccgt
     5941 caccgacatc gcccaccggg tcgagtatgg cgcaaccgga cacgccatcg acgaggcgct
     6001 cagcgacgcc gagctggatg actggttgtt cgccgaatgc agcgacgcgc agcacgcaag
     6061 cggtacctgc cgcatgggcg cggctggcga cccacgctcg gtcgtcgatc ccgaatgccg
     6121 ggtgatcgga tgcaccgggt tacgcgtcat cgacgcttcc atcatgccgg aagtcccgcg
     6181 cgccaacacc catctgacga ccgtcgccat cgccgagcga atggccgacc ggttgaaggc
     6241 cgcgaagcga ggaagaccgg catagaccga gcgtaacaac cagcgccgtg atgatgtgta
     6301 tttttccggc aaatcgtcac agagttcgcg cgatatcctc cacctgcgat ttcatccaga
     6361 gcgcgccttt gtcggatgct gatttcgtcg gcgtcatcaa gtagagaata tcgatgccgg
     6421 gatcgatcgg cggcttgata agcgcaagat caagctcggc ctttcgcgcg gctgcgaagc
     6481 gccgtggcac gaacgcgacg tagtccgtcg cggctgcaat ccgcaacgca atcaggtggc
     6541 ttggcgccgc gacggccacg tttcgctgca gcctcacttt gtcgagccat tcgtcgagtt
     6601 cgtcgcggtc ttctcccgcg cctacgaccg atacgtgttg gctggcctga aagcctttca
     6661 ctgtcgaaag cgcgcgtttg cccggatggc ctgcgcgaac cacaagaata tccttatcct
     6721 catagagcgg tgatctggcg aagccggaaa attcgcgatt gaatgaggtg acgaagaggt
     6781 cgatctcggc cagcgtccgg tctgtcaaca gcgcgggccc ccgccacggg atcaatttga
     6841 cacggatgcc gggcgcggtt ctggcaacgc gtgcgaccag cgatggcata atgaggtgag
     6901 acacgaggtc cggcagcatg aaccggaagg cccgcttgct ggtatgcgga ttgaagtcat
     6961 ccatacgaag aacggcttgc gcgctcgcca cgaattgcgc gagtggcttc cgcagtgcct
     7021 cggcacgagg tgtccgcacc atcgccgtgc cgacgcggac cagcagcgga tcgtcaaaca
     7081 gatcgcggag ccgcgccagc gcatgactgg ccgcgggctg cgagaggccg acgcgctcgc
     7141 cggcgcgccg cacatgcgcc tcgtccagca gggcttccag cagcaccagc aggttcatat
     7201 ccacggcaga taaattcatg gtgttcatgg cttgcataat atcaatcgat tggacgaatg
     7261 aaatgggttc gtacagaaag ctgcgtatga gcgaatggtt gcgagcaaat ccgcccggcc
     7321 ttcctgtcac gccgggatca tcacagatcg cggaagcacg cggtcgcctg atattcatat
     7381 cggggcaggt cgccgtcacc gccgatgatg agcatgtcgg cgcgggagat tttggcgttc
     7441 aactggagca ggtgttccgt aatcttgata cagccgttcg ggctgcaggc ggacggttcg
     7501 aggacatcac gaagctgaac tatttctgtc tcgacagcgt cgatcgcgcc ctgctgcccg
     7561 aagtgcgccg cgtccgggac agctacgtca atgcagaatg cccgccggca agcacgttcg
     7621 tgtttgtctc gggtctggcg cggaaaggct ggttgatcga gatcgaggct gtggctacgc
     7681 ttcgggatca agcgcaatga gtcagcgacc cgatcttgtc cttgtcaccg gcccgatggt
     7741 cggcgcgtca agctgggcac cgacggcgtc gcacctgcgt gcagccggct ggtgtgtcca
     7801 tgttccggat atcctgtcgg caaacggcgc gctgccgccg tggcatgcgc tgtcggcgca
     7861 ttatgcgaag ctgttgtcgc tcgataaccc gccgatcctc gtcggccaca gccttgcgac
     7921 ggtggtggtc gccgatcttg ccgtgtgcgt gtctgctcgt ggcttgatca tggtggatgg
     7981 cgaaatccct cctgccagcg gtcctgtggc gcccggcagg gaatcgttcc gcgcgtttgt
     8041 tctgggtctt gccggcgatg acgggtggct gccccgatgg tcagaatggt ggctcgatca
     8101 tccgagcaga tcggttaccg gcatcgatca gcttgctcat gatgccgatg cgtttgcgtt
     8161 gttcgcgaat gaccagccgc gcgtgccggc ggcatggttc gatgacacga tagatcttgc
     8221 gccgtgggct caaagtcctg cgggatacat ccagacgtcg agattctacg accactccgc
     8281 agaagatgcc gaggcgcggg gctggccgct actgcgattg cagggcactc atctgcatcc
     8341 cacgttacaa ccggatgaga ctgcgctggc tattgccgag atcgcccgca aactggtcca
     8401 cggaagttga cacccggcga taccgcgagc tttttgatgc gtgctgtgcg cgtatgatcc
     8461 gcttccggca agcctcgacc ttacggatcg agctcggtat cccaataaag ataatccagc
     8521 cagctgtcgt gcagatagtt cggcgggaac agccggccgt tgcgatgcag ttgatgcacc
     8581 gtcggcgcga acgggaattg ccgcggaaac atccgcgcct gctgcggcgt caggtttccc
     8641 ttgcgcaggt tacacggcga acacgccgcc accacgttct cccaggtggt ctgcccgccc
     8701 ttgctgcgcg ggatgatgtg atcgaaggtg agatcgtcgt gcgccgaaca atattgacag
     8761 acgaaccgat cgcgcaggaa cacgttgaac cgggtgaagg cgggatgcgt ggtcggcttg
     8821 acgaaggatt tcagcgacac cacgctcggc agctggatct cgaaggaggg gctgcgcacc
     8881 gcgcggtcat agtgctcgac gatgttgacg cgatcgagga acaccgcctt gatcgcgtcc
     8941 tgccacgacc agagagacag cgggtaataa ctcagcggcc ggaaatccgc attcagcacc
     9001 agcaccggcc aaccgccttg cgagacatgt gcgttcaagt aacgcctcca gagcattttc
     9061 cggcaacgta ggaaccggtt tgccgcaaga aatgccccca actataactt gcgcgtactc
     9121 tttcgcaaaa ccggtaccca ctcttgcgga gtacgcgagc cttcagatca gctgcgaagc
     9181 agcatgagtt gacatactac aggcagcgtg acgggattgt gaagagcgtt ggggggctta
     9241 tccagcgggt gaggaaccgt tggcaagcac agaagatacc gccaacggcg ccatccaatg
     9301 cgtcagagaa ccccggagga gccccatgcg cagattgctg acggttatgg tcgcggccct
     9361 tttcggcctg gcagcgaccg gcatggccgc cgcgcagggc tatccggcgc acgcgatgac
     9421 catgatcgtg ccgttccccg ccgggggtgc gaccgacacc tggctcgctt tctgggcgag
     9481 cgtatgcgcg gaattctggg tcaacccgtg atcatcgaga acgttgccgg tgctgccggc
     9541 agtctcggag tcgggcgtgc cgttcgttca gccgccgacg gctacacgct gagcatcggc
     9601 acctcgacca cgcatatgct gaccggcggg ctctacgccc ttcagttcga tctgttgaaa
     9661 gatctcgagc cggttattca aatcggcagc gaaccgcttt tgattgtcgg caagaagagc
     9721 ctgccggcgg cagatctgaa agggctgatc gggtatctca aggccaatcc ggacaaggcg
     9781 tcggtcggca tcgccggcgt cggcgcgaca ggtcatctca ccggaatctc gtttcagaag
     9841 gagaccggaa ccaggtttca attcgtgccg tatcgcggca acgcgccggc catgcaggat
     9901 ctcctggcgg agcagatcga tttcatgatc gagccgtcgt cgaatttcaa atcgctggtc
     9961 ggcgccggaa gcgtcaggcc ttacgcgatc acgggccgga cgcggttgcc ctcgtcaccg
    10021 gatattccga cggcagacga ggcgggcctt cgcggcttct ttgcctcgct ctggtatgga
    10081 ctttgggtgc cgaaggatac gccgaaggat atcatcgcca agctgaatgc gacgatggtg
    10141 caggttcttg ctgatccaat ggtgcgcaag cgcttcgacg aactcggtat cgagatcacg
    10201 ccgctcgcgc agcaatcgcc cgaagccttg cgcgcgttcc agaaggccga atcggaacga
    10261 tggtggccga tcatcaaggc atccaacatc aaggcggaat gacgagtcgg gcttttcggc
    10321 aggcctgctc tcggatagag catgatccgg aaaagcatgc cctcgggcca cgacccgacg
    10381 ggtgggaacc ggttttccga aaagatcatg ctccaacaaa aggatgagat catgatccga
    10441 ttcaatctaa gcggatcatg atctagcatc gcgaaatcgg cgcatttgac tggttcttgg
    10501 ccgccatgaa agtccacgcc attcagacgg ggttcgtccg gatcaaaacc gcgcaggtcg
    10561 aagggcaggg gcacggcctg ccgcggcggc ttgcgatttt tgccgaccgg aactggaccg
    10621 gctggctgcc gacttacgca tggatcatcg atcatccgga aggcatcatc gtggtcgaca
    10681 ccgggcaggg tgcgcatctg ctcgaatccg gcaagtcgct tcacccttat gtccggtggg
    10741 aagtcgggtt tcgcatcgac cgcgaacagg agataggccc gcagctgaag gcgctcggca
    10801 tcgggccgcg cgatgtgaaa cgtgtcgtgc tcacccattt gcatatggac cacgacggcg
    10861 gccttgcgca ctttccgaac agtgaaatcc tggtcgcgcc cggtgaactg cagaccgcaa
    10921 gcggatatgt cggccgcatg cgtggctatc ttccgcatcg atggccgtcc tggttcgatc
    10981 cgaagccgct caatctgaca gccgaatcgt ctggcccctt cgccatgagc cgtcggttga
    11041 caaaagcggg cgacgtcatc gcagtcgcga cgcccggcca caccgcggat catatctcca
    11101 tcatcgtcca ggccagcgag atcacgtatt ttctggccgg cgacacttct tataacgagg
    11161 aactgatgct cgccggcaag ctcgacggcg tcagcgcgaa tgagcaggtc accggcgcga
    11221 cgcttgcggc gatccggcgc ttcacccagt cccgtccaac cgtgtatctg ccgacgcatg
    11281 atcccggtgc cgccgaacgt ctggcaaacc gaggattggc cgcggtcaga gaagggctgc
    11341 tctccaacgc gccggcgtga ggccataggc gcgcttgaac tgtcgtgaca tgtggctttg
    11401 atcggcaaat cccgcgtcaa gcgcggcatc cgcaaggctg ctgccggatt tcaccagccg
    11461 gcgcacctgg tcgagttgcc gcatggtgcg aaaccggctc gggctggtgc cgaaggccgc
    11521 ccggaattgg cgcgcgagcg accagcggtc gagatcggcc acgcgctcca gctcattgat
    11581 cgggtgtcga tcggcgggac gcgcggcaat gcgagcgcga gcgcgcaaca ggccggggag
    11641 cggaagcgcc gacaacggtc tggctgcatc agaacaagcg ctcaaaagaa tatcggcgac
    11701 cgcggtaacg atttcgatgc gcccgatgtc atcgattggg acttccatct cccacgcggc
    11761 cgagagagca tccctctgcc ggttggtcag atcgatgatg ggattcgaca cgaagggaag
    11821 cggcctgccg cccaccgcgc cctgaacgag gcacgggtcg atatagagga tccgataggc
    11881 aaaaccgtct gacgttccgg atgcgccgtc gtggatctcg tcgggatgca ggatgtggca
    11941 ctggccgggc aggcaatgcc gcgcctcgcc gcggtaacga aaggtctgga cgccggcgag
    12001 cgtgatgccg atcgcgtagg tatcgtggcg atgcggtgaa aaggcctgtc cgcgaaaccg
    12061 ggcttcgagc cgttcaatgc cgtcgcatcc caggcccatc gagatgcgcg ccgtctcgcg
    12121 agcaccgccg ggcgtgtgct cgcacaatcc ttcaagaccg tcgctggagc ggcgcgatag
    12181 cgttgcttgc atgtcgacga tgctaacgga atcgcgatga ccgggcacgt gttttcgtca
    12241 ccctggcagt cgtcattgac gctggtcttg gaagccgcgg cgataatggg aacaccgggg
    12301 ccatcgacga tggctgtgat ggcgaccgcc gctgctttcg gcggccgccg cgcgcttggg
    12361 ttcgtatcgg gcgccattgc gggcaccatt gcggttttgc tggccgtcgc cacgggtgta
    12421 gtggcgctcc tggtttcgat ccagggcctc ggctttgtgc tcacgctcgc ctcggccgtc
    12481 tatatcctct atctggcgct gaagatcgcg accgcgccgc cattgccgaa gcagaccgcc
    12541 gaagccgcgg ctccttcgct ggccgcaggc ttcctgctcg ccatcgccaa tcccaaagcc
    12601 tggtttgcca tcgccgcggt gttcaccggt tcaacgctgc tggaatcctc gcgcgaactg
    12661 gatgcgctgc tcaaggcggc tgtgctcgcc gtcatgatcg tcctcatcca tctgtgttgg
    12721 ctgctcggcg gcgtttcact cgccggcttt ctccgcgatc ccctgcgatc gcgcattgtc
    12781 aacgttctgt tcggcctggt cctggtcgga accaccgcgc tagcactcat ccgttgaatg
    12841 ctaaattctc tccagcttct gcaggcagcg gaccaccgcc ggcatcggcg tatccggaaa
    12901 actcgtcttc cagttggtga cgccgacttc gccgacatag atgtcgccct tcgaatccag
    12961 ggcgatgccg tgcggcgcga gaaatttgcc ggtctcgagg ccgggaccat tctcgccgcc
    13021 gagccgcgcg atgcgctttc cttttgaatc gacgatgctg aggcgcgggc ccaaattcgg
    13081 cactttcagg ttcaccggca tgccggggcc gagctcgccg atgatgaagt taggctgctt
    13141 gccgccgcag caatgcagcg cgcagggccg gtgcagatta ttccattgcg cctcgtactt
    13201 gccgttgccg tcgaacacct gcacgcggtg gttttcgcgg tcggccacat acacccagcc
    13261 atcctggtcc gtgacgatgt tgtgcacaat gttgaactgg cccggatcgg tgccgggctc
    13321 gccccaggtt ttcagcaatt tgccgtcagg cgtatatttg tggacgcagg cattgccgta
    13381 gccgtccgac acgtagatct cgcccttggg cgacagcgcc gtgtgggtgc agcggtgaaa
    13441 cggttcgccg ctcatgaacg gcgcgggctt gttcgggatg ccgatcgtca gcag
//
LOCUS       DATNOI010000514         3896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_28326,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000514 DATNOI010000000
VERSION     DATNOI010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28326"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VN968_20715"
     CDS             <1..826
                     /locus_tag="VN968_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-gated ion channel"
                     /protein_id="HXL31693.1"
                     /translation="FLKLVRYSPALRSLLAALHAERRTLFGCVVILTGAVLLFASLLY
                     AIEHQVQPDKFGTIPQAMWWAIVTLGTVGYGDVVPVTALGKLVSVFTIIGGLTMIALP
                     VAIISTAFADEVRRRDFVVTWGMLARVPLFSHLGAAEIADIMRLLRAQTIESGEILVR
                     RGDAASSMYFITAGEVEIELPGQRVRLSDGTFFGEIALLHRTKRSGTVTARRKTKLLA
                     LGAQDFHALIERLPDLAAQIHRTAKARLADTEQARKGDLAAGEIAQAAHTDKPADE"
     gene            complement(1100..1489)
                     /locus_tag="VN968_20720"
     CDS             complement(1100..1489)
                     /locus_tag="VN968_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018447160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL31694.1"
                     /translation="MIGYITIGAADIERAKTFYDSVLSTIGWKQFADYGDPVGYAQNE
                     TGEGQTIWICKPFDGAAARAGNGIMVGFDAETKEQVHAFHDAAMKAGGSDEGGPGPRP
                     DYGPNWYAAYLRDPTGNKLAVVCRKWA"
     gene            complement(1555..1839)
                     /locus_tag="VN968_20725"
     CDS             complement(1555..1839)
                     /locus_tag="VN968_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HXL31695.1"
                     /translation="MLFMVIERFKDRDPIPVYKRVRDEGIKFPEGLRYVGSWIEPNFD
                     RCFQLMECDDARLLQDWILNCQGFGMTFEIVPVVPSKETREVVAPFLDSK"
     gene            complement(1932..2438)
                     /locus_tag="VN968_20730"
     CDS             complement(1932..2438)
                     /locus_tag="VN968_20730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HXL31696.1"
                     /translation="MVFDGKAFLPLLLLSSSLLSARAETPLPDTIAAPGEATVLTVHA
                     EGAQIYECRAGADGKPAWAFREPIATLLVDGKTVGRHYSGPNWEHSDGSAVVGKAAGN
                     APGATPNDIPWLKLEVISHRGSGILTAATTVQRINTIGGKLEGACDKAGAFKSAPYSA
                     DYVFLHKG"
     gene            complement(2556..3767)
                     /locus_tag="VN968_20735"
     CDS             complement(2556..3767)
                     /locus_tag="VN968_20735"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HXL31697.1"
                     /translation="MAKIKVTNPVVELDGDEMTRIIWQYIKDKLINPFLDIDLMYFDL
                     GMEYRDKTNDQVTVDAANAIKKVGVGVKCATITPDEGRVKEFGLKEMWKSPNGTIRNI
                     LGGVVFREPIICKNVPRLVPGWTKPIIIGRHAFGDQYRATDLKFPGKGTLTMKFVGDD
                     GHVIEREVYKSPSAGVALAMYNLDDSIADFARASFNQGLSKGYSVYLSTKNTILKVYD
                     GRFKDIFQEIFDAEFKAKFDEKKITYEHRLIDDMVAAAMKWSGGYVWACKNYDGDVQS
                     DTIAQGYGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYREHQKGKETSTNSIASIFAW
                     TRGLSHRAKLDDNDALAKFALTLERVCVDTVEAGYMTKDLALLVGAEQRWLSTTGFLD
                     KVAENLTKAMA"
BASE COUNT          657 a         1202 c         1233 g          804 t
ORIGIN      
        1 gttcctcaaa ctcgtgcgct attcgccggc gctgcgctcg ctgctcgccg cgcttcacgc
       61 cgagcggcgc acgctgttcg gctgcgtcgt gatcctgacc ggcgcggtgc tgctgttcgc
      121 ctcgctgctt tatgcgatcg agcaccaggt gcagcccgac aagttcggca ccatcccgca
      181 ggcgatgtgg tgggcgatcg tgacgctcgg cacggtcggc tatggcgacg tagtgccggt
      241 gacggcgctg ggcaagctgg tgtcggtgtt caccatcatc ggcgggttga cgatgatcgc
      301 gctgccggtg gcgatcatct cgacggcgtt tgccgacgaa gtccggcggc gcgattttgt
      361 tgtcacctgg ggcatgctgg cgcgggtgcc gctgttctcg catcttggtg ctgcggagat
      421 cgccgacatc atgcggctgc tgcgggcgca gaccatcgag tcaggcgaaa tcctggtgcg
      481 gcgcggcgat gccgcttcct cgatgtattt catcaccgcg ggcgaggtcg agatcgaact
      541 gccgggccag cgcgtccggc tttccgacgg cacgttcttt ggcgaaatcg cgctgctgca
      601 tcgtaccaag cgcagcggca ccgtgacggc gaggcgaaaa acaaagctgc tggcgctcgg
      661 cgcgcaggat tttcacgcgc tgatcgagcg tttgcctgat ctggccgccc agattcaccg
      721 gaccgcgaag gcacggcttg ccgataccga gcaggcgcgc aagggcgatc tcgcggcggg
      781 tgaaattgcg caagcagcgc atacggataa gcccgcggac gaatagttgg gacgagccgg
      841 gcggctcgct cgcggcgggc tttcctgcac gggattgtca gtctgttgca gcgggaggcc
      901 agcatgctcc ctcggggcct gaggcagaag gtcactaccg ctcatcattc aagatagttg
      961 gcaacagaaa cggccctcgc gtcgggctga ccacgcaggg gccatctgat cgaacgccgc
     1021 ggagcggccc gcgacatcca caaccaggca accactttcc tgatttccaa ctcagttcac
     1081 ccggttcggt gaagttgcct tacgcccact tcctgcacac gaccgcgagc ttgttgccgg
     1141 tgggatcgcg caggtaagcg gcataccagt tcgggccgta atccggccgt gggccggggc
     1201 cgccttcatc gctgccgccg gccttcatcg cggcgtcgtg aaacgcgtga acctgctcct
     1261 tggtttcggc atcgaaaccg accatgatgc cgttgccggc gcgcgccgcc gcgccatcga
     1321 acggcttgca gatccagatg gtctggcctt cacccgtttc attctgtgcg tagccgaccg
     1381 ggtcgccata atcggcgaac tgcttccagc cgatggtgct gagcacggaa tcgtagaacg
     1441 ttttggcccg ctcgatgtcg gcggcgccga tggtgatgta gccgatcatg gttcgctccc
     1501 ttaagtgggg agcagactag cagcgagata gaacaaatca agaacaaatt cgggttactt
     1561 cgaatccagg aacggcgcga cgacttcgcg ggtttccttg ctcggcacca ccgggacgat
     1621 ctcgaaggtc atgccgaaac cctggcagtt caggatccaa tcctgcagca gccgcgcgtc
     1681 gtcgcattcc atcagctgaa agcagcggtc gaaattgggc tcgatccagc tcccgacata
     1741 gcgcagtcct tcgggaaatt tgatgccctc gtcgcgcacg cgcttgtaga ccgggatcgg
     1801 gtcgcggtcc ttgaagcgct cgatcaccat gaacagcatc ggcaacccct tatcgatcgg
     1861 tgtcgtgcag cgttgttcag gacgcttcgc cggtcaagcc ttgtcgcttg ggtctcgtcg
     1921 cctaagcctg ctcagccctt gtgcaggaac acatagtcgg cggagtaggg cgcgctcttg
     1981 aaggcgccgg ccttgtcgca ggcgccctcg agcttgccgc ctatcgtgtt gatccgctgc
     2041 accgtggtgg ctgctgtgag gatgccgctg ccgcggtggg aaatcacctc gagcttgagc
     2101 cagggaatat cgttcggtgt cgcgcccggg gcgttgccgg cggcctttcc gaccaccgcg
     2161 ctgccgtcgc tgtgttccca gttcggcccg gaatagtgcc ggccgacggt tttgccatcg
     2221 accagcaggg tcgcaatcgg ctcgcgaaac gcccaggctg gcttgccgtc ggcgccggcc
     2281 cggcattcgt agatctgcgc gccttcggcg tgcacagtaa gcacggtcgc ttctcccggc
     2341 gccgcaatcg tgtcgggaag cggcgtctcg gcccgcgcgc ttaggagtga gcttgacagc
     2401 aggagcagcg ggagaaaagc cttgccatca aacaccatcg tcgcactctc cctcattgat
     2461 ttcgaacaac aggcagtccg cttccgctcg caagaccagc acccaacctc ggagcaggtc
     2521 cgagggcatg ctttttgtag atgatatgcg acggcttacg ccatcgcctt ggtcaggttt
     2581 tccgcgacct tgtcgaggaa tccggtggtg gacagccagc gctgctcggc gccgaccaac
     2641 agcgcgagat ccttggtcat gtagccggct tcgacggtat cgacacaaac cctctccagc
     2701 gtgagcgcga atttcgcgag cgcgtcattg tcgtcgagct tggcgcgatg cgacagcccg
     2761 cgggtccagg cgaagatcga ggcgatcgag ttggtcgagg tctccttgcc cttctggtgc
     2821 tcgcggtaat gccgcgtcac ggtgccgtgg gcggcctctg cctccaccgt cttgccatcc
     2881 ggcgtcatca agaccgaggt catcaggccg agcgagccat agccttgcgc gatggtgtcg
     2941 gactgcacgt cgccgtcata gttcttgcag gcccagacat agccgccgga ccacttcatg
     3001 gcggcggcga ccatgtcgtc gatcaggcga tgttcgtagg tgatcttctt ctcgtcgaat
     3061 ttggccttga actcggcgtc gaaaatctcc tggaagatgt ccttgaaccg cccgtcatag
     3121 accttgagga tggtgttctt ggtcgacagg tacaccgaat agcccttcga caggccctgg
     3181 ttgaacgagg cgcgggcgaa atcggcgatc gagtcatcga gattgtacat cgccagcgcg
     3241 acgccggcgc tcggggattt gtagacctcc cgttcgatca catggccgtc gtcgccgacg
     3301 aatttcatcg tcagcgtgcc cttgcccgga aacttcaaat cggtggcgcg gtactggtcg
     3361 ccgaaggcgt ggcggccgat gatgatcggc ttggtccagc ccggcaccag ccgcggcacg
     3421 ttcttgcaga tgatcggctc gcggaacacc acgccgccca gaatgttgcg gatggtgccg
     3481 ttcggcgact tccacatttc cttcaggccg aattccttca cccggccttc gtccggcgtg
     3541 atggtggcac atttgacgcc gaccccgacc ttcttgatgg cgttggcggc gtccacggtg
     3601 acctggtcgt tggtcttgtc gcggtattcc atcccgaggt cgaaatacat caggtcgata
     3661 tcgaggaacg ggttgatcag cttgtccttg atgtattgcc agatgatccg ggtcatctcg
     3721 tcgccatcga gttcgacgac ggggttggtc accttgattt ttgccatggc aggtcgggcc
     3781 tttttggaaa acagcgcgtt tcgggcgggg cgggatgatc gcatccgcgc tatagcactg
     3841 ccgacgcgct ggcgaaagcc aaggcgttag gcagtttcag cccatctttt ggttta
//
LOCUS       DATNOI010000515         3841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2386676,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000515 DATNOI010000000
VERSION     DATNOI010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2386676"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="VN968_20740"
     CDS             <1..920
                     /locus_tag="VN968_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HXL31698.1"
                     /translation="YDAAHMLAPMPIAISLGLGAQPIPFTVPAIENINGQGITLAIKH
                     KDRRDPKDWKGMKFAIPFDYSMHNYLLRYYLAEHGVDPDTDVQLRSVPPPEMVANLRA
                     DNIDGFLAPDNICQRAIYDGVGFLHILSKEIWDGHPCCSFAASREFITTSPNSFAALT
                     RAIIDATAYASKPENRKQIAEAIAPPNYINAPITVLEQVLTGIYADGLGGVKTDAKRV
                     DFDPFPWQSFAVWMLTQMKRWGQVSGEVDYKSVAEQVYLATGTSKVMKDMGMTPPATS
                     YKSFSVMGKPFDPSKPDEYVASFKIRKSS"
     gene            complement(979..2007)
                     /locus_tag="VN968_20745"
     CDS             complement(979..2007)
                     /locus_tag="VN968_20745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXL31699.1"
                     /translation="MLDITYSPSNEDESSQAARQAAAGDDALLDAYSNAVISVTERVE
                     PAVVRVETGPKVRNAHERGGLGSGIVISPDGLVLTNSHVVGSSKQIRLRDIEGIVTDA
                     RVLGVDADTDLALLRADGVRDLRYASLGNSKTLRRGQLVVAIGNPLGFESTVTAGVVS
                     ALGRSIRSVSGRTIEDVIQTDAALNPGNSGGPLVSSAAEVIGINTAIISGAQGICFAV
                     ASNTAQFVLSEIIRHGYVRRAYIGVSGQTAPIPRRHAILAGVENKMGALVAQIEPEGP
                     AARAGLLPGDVVIKLDGADVNGVDDLIRALDRDRIDRTLEMDVLRMGRLRAVDIHPIE
                     RKPSARQG"
     gene            complement(2124..2756)
                     /gene="pncA"
                     /locus_tag="VN968_20750"
     CDS             complement(2124..2756)
                     /gene="pncA"
                     /locus_tag="VN968_20750"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HXL31700.1"
                     /translation="MQVRPGDNDLLLIIDVQNDFCPGGALAVADGDAVVPVINRLAER
                     FAHIALTQDWHPRGHSSFATSHPGLAPFETISMPYGQQTLWPNHCVQGTPGAAFHPQL
                     ATERAELVIRKGFRPEIDSYSAFYENDRRTPTGLAGYLRERGFTRIFLAGLATDFCVH
                     YSAMDARRLGFESVVVEAGCRAIDLAGSLDAAWTVMTAAGVQRVKDLAAI"
     gene            complement(2863..3636)
                     /locus_tag="VN968_20755"
     CDS             complement(2863..3636)
                     /locus_tag="VN968_20755"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HXL31701.1"
                     /translation="MSNFNQESVVSVHHWTDRLFSFTTTRHPSFRFRHGEFTMIGLKV
                     GERPLLRAYSVASANYEDRLEFFSIKVLDGPLTSRLQHLKQGDAIIVSRKATGTLVID
                     NLEDGRNLYLIGTGTGLAPFLSVIKDPETYERFEKVVLLHGCRRVAELAYGEMITEKL
                     PNDELIGDLVRNQLIYYPTVTRDPFRNRGRITELMTSGKLFSDVGLAPLAAKQDRVMI
                     CGSPALIHDTRALLLARDFVEGNHGEPGQFVVEKAFAER"
BASE COUNT          754 a         1210 c         1181 g          696 t
ORIGIN      
        1 aatacgacgc cgcgcacatg ctggcgccga tgccgatcgc gatctcgctc ggcctgggcg
       61 cacagcccat ccccttcacg gtgccggcga tcgagaacat caacggccag ggcatcacgc
      121 tcgccatcaa gcacaaggac aggcgcgacc cgaaggactg gaaaggcatg aaattcgcca
      181 ttccattcga ttattcgatg cacaattatc tgctgcgcta ctatctcgcc gagcacggcg
      241 tcgatcccga caccgacgtg caattgcgtt cggtgccgcc gccggaaatg gttgccaatc
      301 tccgcgccga caacatcgac ggcttcctgg cgccggacaa catctgccag cgagcgatct
      361 acgacggcgt cggtttcctg cacattcttt ccaaggaaat ctgggacggg catccctgct
      421 gcagctttgc cgcgagccgg gagttcatca cgacatcgcc caatagtttc gccgcgctga
      481 cgcgcgccat catcgacgcc actgcctacg cgtccaaacc cgaaaatcgc aagcagatcg
      541 cggaagccat cgcaccgccg aactatatca acgcgccgat cacggtcctt gagcaggttc
      601 tcaccggcat ctacgcggac ggacttggcg gcgtcaaaac ggacgccaag cgcgtcgact
      661 tcgacccgtt tccctggcag tcgtttgcgg tctggatgct cacgcaaatg aagcgctggg
      721 gccaggtatc cggtgaagtg gattacaagt ccgtggcgga gcaggtctat ctcgcgaccg
      781 gcaccagcaa ggtcatgaaa gacatgggaa tgacgccgcc ggcaacgtcg tacaagtcgt
      841 tttcggtgat gggaaagcca ttcgacccgt caaagcccga cgaatacgtg gcaagcttca
      901 agatcaggaa atcatcctga cgcgatagcc tctcacaatc gcgcccgcct gtttctcgca
      961 cccgcggctc tacggggctc agccctgccg agccgacggc ttgcgttcga tcggatgaat
     1021 gtcgacggcc cgcagccggc ccatgcgcag cacatccatc tcgagcgtgc gatcgatgcg
     1081 gtcgcgatca agcgcacgga tcagatcgtc gacgccgttc acgtcagcgc cgtccagttt
     1141 gatcacgacg tcgccgggca atagcccggc ccgcgccgcc ggcccctccg gttcgatttg
     1201 cgccaccagt gcgcccatct tgttttcaac accggcgagg atcgcatgcc ttctcgggat
     1261 tggcgcggtc tgaccggata caccgatata ggcgcgccgg acatagccgt gacggatgat
     1321 ttcggacaat acgaattgcg cggtgttgct ggcaaccgca aaacagatgc cctgggcgcc
     1381 gctgatgatc gcggtgttga tgccgatcac ctcggccgcg gaggacacca gcggtccgcc
     1441 ggagttgcca ggattaagcg cggcgtcggt ctggatgacg tcttcgatgg tcctgccgct
     1501 cactgagcgg atcgaacggc cgagcgccga caccacgccg gcggtgacgg tcgattcgaa
     1561 gccgagcgga ttgccgatcg cgaccaccag ctggccgcgg cgcagggttt tggaattgcc
     1621 gagcgaggcg taccgcaaat cccgcacgcc gtcggcgcgc agcaacgcca gatcggtgtc
     1681 ggcgtcgacg ccgagcacgc gcgcatcggt gacgatgccc tcgatgtcgc gcagcctgat
     1741 ctgtttggac gaaccgacca cgtggctatt ggtcagcacc agtccgtcag gcgagatcac
     1801 gatcccggag ccgagaccgc cgcgctcgtg cgcgttgcgt accttcggcc cggtttcgac
     1861 ccgaaccacc gcgggctcga cgcgttcggt cacgctgatc acggcattgg aataggcgtc
     1921 gagcaaggcg tcatcgccgg cggcggcctg cctggccgct tgcgatgact cgtcttcatt
     1981 tgaagggctg taggtaatat ccaacatggc gaattccttg gccctgtcaa atggtgacct
     2041 tgatttgtcc gcgcaagggg gagcggtctg gcaggcgcgg cagcggttag tggaataccc
     2101 ggacgacgcg gaccgcgtca gaatcagatc gccgcaaggt ccttgacgcg ctgcacgccg
     2161 gctgcagtca ttacggtcca ggcggcatcg agcgagcctg ccagatcgat ggctctgcag
     2221 ccggcctcga caacaacgga ctcgaagcca agcctgcgcg cgtccatggc ggagtaatgg
     2281 acgcaaaagt cggtcgcgag cccggcgagg aatatccgtg taaaaccgcg ctcgcgcaga
     2341 tagcccgcga gccccgtggg agtgcgccgg tcgttttcat agaacgcgga gtaggaatcg
     2401 atttcggggc gaaacccttt ccggatcacg agctccgccc gctcggtcgc cagttgcgga
     2461 tgaaaggccg cgcccggcgt gccctggacg caatgattgg gccacagcgt ttgctggcca
     2521 taaggcatgc tgatggtttc aaacggcgcc aagcccggat gggatgtggc aaaggagctg
     2581 tggcctcggg ggtgccagtc ctgggtcagc gcgatgtgag caaaccgttc ggcgaggcga
     2641 ttgatgacgg gcaccacggc atcgccgtcg gcaaccgcga gcgcgccgcc gggacagaaa
     2701 tcgttttgca cgtcgatgat cagcagcaga tcattgtcgc cgggccgcac ctgcatgttg
     2761 cacctgcgtt acattcgacg tgcaaacttt agcgcatgcc agaagctctg gcagcagggg
     2821 aattgcgggc cgtccgatac gcgcggctcc ctccccgctt gctcagcgtt cggcgaatgc
     2881 cttctcgacc acgaactgac cgggttcgcc gtgattgccc tcgacaaaat ccctggccag
     2941 caatagcgcc cgtgtgtcgt ggatcagcgc cgggctgccg cagatcatga cgcgatcctg
     3001 tttcgccgcg agcggagcta ggccgacatc gctgaacaat tttccggaag tcatcagctc
     3061 agtaatgcgg ccgcgattgc ggaacgggtc gcgcgtcacg gtcggataat agatcagctg
     3121 gttgcgcacg aggtcgccga tcagctcgtc gttcggcaat ttttcggtga tcatctcgcc
     3181 ataggcgagc tcggcgacgc ggcggcagcc gtgcagcagc acgactttct cgaagcgctc
     3241 gtaggtctcg ggatccttga tcacgctcag gaacggcgca agcccggtgc cggtgccgat
     3301 cagatacaga ttgcggccgt cctcaagatt gtcgatcacc agcgtgccgg tcgccttgcg
     3361 gctgacgatg atcgcgtcgc cctgcttgag atgctggagc cgcgaggtca gcggcccgtc
     3421 cagcaccttg atcgaaaaga attcgagccg gtcctcgtaa ttggcgctgg cgacgctgta
     3481 ggcgcgcagc agcggccgct cgccgacctt caacccgatc atggtgaatt cgccgtggcg
     3541 aaagcggaac gacggatggc gcgtggtggt gaagctgaac aatctgtcgg tccagtgatg
     3601 aacgctgaca acgctttcct gattgaagtt gctcatgatt ccgccctctg caggtagatt
     3661 tgcgctggtg cactcattcg tacacgatca tttgtatact aatgaataat ccagcttgat
     3721 cgatccgtca agccgcgcgc aagatgccgc gccacgcggc ccgcgaaggc attgaacaac
     3781 aaggaaaaat ccatggcgca cgacgaaccc caggccgccg gccccacaaa actcgtgatc
     3841 c
//
LOCUS       DATNOI010000516         8243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2520785,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000516 DATNOI010000000
VERSION     DATNOI010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8243
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2520785"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="VN968_20760"
     CDS             <1..1240
                     /locus_tag="VN968_20760"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HXL31702.1"
                     /translation="AAADLAWWDAPFIGPRKAWGAEPVRFQWSVPEPTRIALMNSLVE
                     RFNASQKDFAVQMEYVPQAQARQKLISSIAGGSPPDLCQVWDNWVGQFNGMGAVEDIT
                     ARAKSWKHYADVAPTAWQTVTINDKIISLPLAVTLDGIYYRTDRLKELGLKEPTPDWT
                     WDEFLSLAKAFTKVDKNQYGYGMRGGGTWALLYPSEFAYANGAEVLKDGKVVINSKEA
                     VDALAWYLDLALKHKVTPPSAATDGFVEIVETFGRGVTSMYQHNSGSSGQQKKNVGAD
                     NFATLPVPIGPANKRASFWFSETLTMFKGARNKEGAWQFMSFMLEDEPSLKYNSTLGL
                     LPARQSILKQPEFAKDPTLAGFVNSFGIGIVSPYLAYPGWGGKIDSDGVPLIQQAILG
                     KISAKDCLDRFAEILKKEMS"
     gene            1442..2371
                     /locus_tag="VN968_20765"
     CDS             1442..2371
                     /locus_tag="VN968_20765"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL31703.1"
                     /translation="MTTLTLAKPRTRPLISWRMRKLLIGYSYLVPAALCMLATVVVPI
                     ILAIKMSLYADVLYKPQDYRFIGLGNYVRLVQDPVFWLTLWNSVVWVFGSVILQFVFG
                     FAAALLLQQAFRGRALVRTLTLLPWIIPGVVVGLIWEWLYQPNYGVINDLLIRAGWMH
                     ERLAWLSSPDTAMAAVVFTNVWRGIPFFAIMLLAGLQAVPDELYEAAHVDGAGVLERF
                     WHITLPLLRPIIVVATATRIIWTFNYADLIFVMTGGGPANATQITSTYTLLQAYSSLD
                     FGYAGALSVVLLIVMLTFTALYLRVTKGVEETE"
     gene            2368..3231
                     /locus_tag="VN968_20770"
     CDS             2368..3231
                     /locus_tag="VN968_20770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL31704.1"
                     /translation="MTRRHVPKDLAERLLGGPVVWIGLVLLTAFALGPFIWMLLTSLK
                     DRQELYATPLQYLPVRPTFENYVDAWTSPVTPFSRFFLNSLWVCSVTMVATTVVSVLA
                     GYAIARFRFAGRDALLLIFLATQMFPSVLLIAPLLTQWRALGLVDTYQALIYSNFSFT
                     VPFTVWMMVGYFSSIPRDLEESAMIDGCNHFGALCRIVLPLAAPGIAATAIFAFVVSW
                     SELLFAISFTTETSMRTLSAGLLYMVGQYEVQWGPLSAGVILSTVPVAVLFSFLQRHL
                     IHGLTAGAVKG"
     gene            complement(3501..4292)
                     /locus_tag="VN968_20775"
     CDS             complement(3501..4292)
                     /locus_tag="VN968_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HXL31705.1"
                     /translation="MIEAKMRRRGVAGFGLFTLAMVTLFVGLGVWQLQRRVEKHALIA
                     ALSERLAATPGPLPSPSQWSALTPANDEFRRISFAATYESRPDAMVYSSGSGIREDIS
                     GPGTWAFMPARLPGGETVVVNAGFVPNTMQDREQQDRAVARLVTKERVNLTGYIRFPE
                     AAGLLTPPENPAKRLWFTRDHLAMARALGWGEGGAPVAPFYIDLEQPVPASGIPKPGA
                     LEVHLKDDHLQYAITWFALAGAVVIAFAVWLRAQRRAAAFREQDV"
     gene            complement(4289..4675)
                     /locus_tag="VN968_20780"
     CDS             complement(4289..4675)
                     /locus_tag="VN968_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HXL31706.1"
                     /translation="MQDQTPTVTESAMRGLACKCPRCGRGKLYAGFLNLRPNCEACGL
                     DYAFIDSGDGPAVFIIMIAGAVVVGCALIVEIKYQPPFWLHAVLWLPLILATTLLPLR
                     SMKSLLIALQYHHKAAEGRLIDREPK"
     gene            complement(4802..5659)
                     /locus_tag="VN968_20785"
     CDS             complement(4802..5659)
                     /locus_tag="VN968_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HXL31707.1"
                     /translation="MATAQVKHHDYHLVDPSPWPIVGAVSAFIMAVGAIAWMHHMFAA
                     APIIFGVGTIGVLYTMAGWWTDVIREAQYKGDHSRVVQISHRYGMILFIASEVMFFVA
                     WFWAFFNSALFPADPVHATREALFGGVWPPKGIETFDPWHLPLLNTLLLLTSGTTVTW
                     AHHALLENDRQGLKYGLMLTIALGACFTCVQAYEYAHAAFSFAGNIYGATFFMATGFH
                     GFHVLIGTMFLTVCLVRAYLGHFSPTQHLGFEFAAWYWHFVDVVWLFLFICIYVWGHG
                     AETMAHGAH"
     gene            complement(5756..6394)
                     /locus_tag="VN968_20790"
     CDS             complement(5756..6394)
                     /locus_tag="VN968_20790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HXL31708.1"
                     /translation="MPQAPTRSLEETSRRGSRRGLGRDAAVAAICGLIVVLMVGASYA
                     AVPFYNWFCRTTGFNGTTQVATSAPSSAPLARKIAVRFDANVAAGLPWKFEPEQTEIE
                     VRIGEVVTVFYTVTNQAARATTGQAAYNVAPLTVGAYFQKINCFCFTEQTMAAGETRE
                     MPVVFYVDPALVKDSDNDGLNGITLSYTFYPVRDPAARPLAAGEPDKREGRL"
     gene            complement(6418..6582)
                     /locus_tag="VN968_20795"
     CDS             complement(6418..6582)
                     /locus_tag="VN968_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoxF protein"
                     /protein_id="HXL31709.1"
                     /translation="MDDKREPDGIVLTEAQKKSRRQRSIAIALALGVLAVLFFAVTLV
                     KGPAVLVRPM"
     gene            complement(6603..7541)
                     /locus_tag="VN968_20800"
     CDS             complement(6603..7541)
                     /locus_tag="VN968_20800"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HXL31710.1"
                     /translation="MSVVDRNAIELSPRISEADVADYIALLKPRVMSLVIFTALVGLV
                     LAPGHFHPVLAFTSLLCIAVGAGASGALNMAYEGDIDAKMSRTANRPIPRGRITQAEA
                     LTFGTTLAFFSVMTLGILVNWFAGALLAFTIFFYVVIYTIALKRWTAQNIVIGGAAGA
                     LPPVVAWAAATGSLSVEPILLFLIIFFWTPPHFWALALFRSDDYARAGVPMLPVVAGP
                     DATRLQILLYTIVLIAVAFAPWPLGYFDAVYGVTSLVLGGAMLALAVSVYRRREGSEA
                     RRATRKLFAFSILYLFGLFATLLLEVVVRALAPTIW"
     gene            complement(7593..>8243)
                     /locus_tag="VN968_20805"
     CDS             complement(7593..>8243)
                     /locus_tag="VN968_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXL31711.1"
                     /translation="SATQAYFVAATMVIAVPTGVKIFSWIATMWGGSIEFKTPMLWAI
                     GFIFLFTVGGVTGVVLANAGVDRVLQDTYYVVAHFHYVLSLGAVFGIFAGWYYWFPKM
                     SGYMYSETIGKLHFWFTFIGVNLVFFPQHFLGLSGMPRRYVDYPDAFAGWNLVSSIGS
                     YISGFGVLIFLWGLVDAFVRKQQAADNPWGAGATTLEWTLPSPPPFHQFEVLPRVQ"
BASE COUNT         1580 a         2651 c         2564 g         1448 t
ORIGIN      
        1 cgccgcagcc gatctggcct ggtgggatgc tccgttcatt ggcccccgca aagcgtgggg
       61 cgccgagccg gtgcgctttc agtggagcgt tcccgagccg acgcgcatcg cgctgatgaa
      121 ctcgctggtc gagcgcttca atgcgtcgca gaaggatttt gcggtgcaga tggaatacgt
      181 gccgcaggcg caggcgcgcc agaagctgat cagctcgatc gccggcggaa gtccgcccga
      241 tctgtgccag gtctgggaca actgggtcgg ccagttcaac ggcatgggcg cggtcgagga
      301 catcaccgcg cgggcaaaat cctggaagca ctatgcggat gtggcaccga cggcgtggca
      361 aaccgtaacc atcaatgaca agatcatctc gcttccgctg gccgtgacgc ttgacgggat
      421 ttactaccgt accgaccgcc tgaaagagct cggcctgaag gagccgacgc cggactggac
      481 ctgggacgag ttcctcagcc tcgcgaaagc cttcaccaag gtggacaaga accagtacgg
      541 ctacgggatg cgcggcggcg gcacctgggc gctgctctat ccgagcgagt tcgcctacgc
      601 caacggcgcc gaagtgctca aggacggcaa ggtggtgatc aattcgaagg aggccgtcga
      661 cgcgctcgcc tggtatctcg accttgcgct caagcacaag gtcacgccgc cgagtgccgc
      721 gaccgacggc ttcgtcgaaa tcgtcgaaac ttttgggcgc ggcgtcacca gcatgtacca
      781 gcacaattcc ggctccagcg gccagcagaa gaagaacgtc ggcgccgaca atttcgccac
      841 gctgccggtg ccgatcggtc ctgccaacaa gcgcgcctcg ttctggttct ccgagacgct
      901 caccatgttc aaaggcgcca ggaacaagga aggcgcgtgg caattcatga gcttcatgct
      961 cgaggacgag ccgagcctca aatacaactc gacgctcggc ctgctgccgg cgcgccagtc
     1021 gatcctcaag cagccggaat ttgccaagga cccgacgcta gcgggcttcg tgaactcttt
     1081 cggaatcggg atcgtcagcc cctatctcgc ctatcccggc tggggcggca agatcgactc
     1141 cgacggcgtg ccgctgatcc agcaggcgat ccttggaaag atctccgcga aagattgcct
     1201 cgaccgcttc gccgaaatcc tgaaaaagga aatgtcgtag gcgtttgacc cagaatgcag
     1261 ccaatgaaat tgggcattcc gacgcaactc gtcatgtccg gccttgggca gccttgggcc
     1321 gggcatccac ttcttagctg caccaggtaa gaaagacgtg gatggccggg acgtctagcg
     1381 cgaagacgcg cttcgcgctt ttgcccggcc atgacgaaaa agcgaatcag caggaaccat
     1441 catgaccacg ctcaccctcg caaagccgcg cacccgcccg ctgatcagct ggcggatgcg
     1501 caagctcctg atcggctatt cgtatctggt gccggccgcg ctgtgcatgc tcgcgaccgt
     1561 cgtcgtgccg atcatcctcg cgatcaagat gagcctctat gccgacgtgt tgtacaagcc
     1621 gcaggactac cgcttcatcg ggctcggcaa ctacgttcgc ctggttcagg accccgtgtt
     1681 ctggctgacg ctgtggaatt ccgtggtctg ggtgttcggc tcggtcatcc tgcaattcgt
     1741 gttcggattt gcagccgccc tgctgctgca gcaggccttc aggggccgcg ctttggtgcg
     1801 cacgctcacg ctgttgccct ggatcatccc cggggtcgtg gtcggactga tctgggaatg
     1861 gctctatcaa ccgaactacg gcgtcatcaa tgacctgttg atccgcgcgg ggtggatgca
     1921 cgagcgcctc gcctggctgt cctcgccgga cacagcgatg gcggcggtcg tcttcaccaa
     1981 tgtctggcgc gggatcccgt tcttcgccat catgctgctg gcggggctgc aggccgtgcc
     2041 ggacgagctt tacgaagcgg cccatgtcga cggcgccggc gtgctggagc gtttctggca
     2101 cattaccctg ccgttgctgc ggccgatcat cgtcgtcgca accgccacgc ggatcatctg
     2161 gaccttcaac tatgccgacc tgatctttgt catgaccggc gggggacctg ccaacgccac
     2221 ccagatcacg tcgacctata ccttgctgca agcctattcg agccttgact tcggctacgc
     2281 cggcgcgctc tcggtcgtcc tcctcatcgt gatgctgaca ttcaccgcgc tttacctccg
     2341 cgtcaccaaa ggcgtcgagg aaaccgaatg acccgccgcc acgtcccgaa agaccttgca
     2401 gagcgactat tgggcggccc ggtggtctgg atcggcctgg tgctgctgac cgcgtttgcg
     2461 ctcgggccgt tcatctggat gttgctgaca tcgctgaagg atcggcagga actctacgcg
     2521 acgccgctgc agtaccttcc ggtgcgccct accttcgaga attatgtcga tgcctggacc
     2581 tcgccggtca cgccgttttc ccggttcttt ctcaatagcc tctgggtgtg ttcggtcacg
     2641 atggtcgcca ccacggtggt gtcggtgctt gccggctatg ccatcgcccg ctttcgtttc
     2701 gccggaagag atgcgctgct gctgatcttt cttgccaccc agatgtttcc gagcgtgctg
     2761 ctgatcgccc cgctcttgac gcaatggcgc gcgctcggcc ttgtcgatac ctaccaggcg
     2821 ctgatctact cgaacttttc gttcaccgtg ccgttcacgg tctggatgat ggtcggctat
     2881 ttcagttcga taccgcgcga cctcgaggaa agcgcgatga ttgacggctg caatcatttc
     2941 ggcgcgcttt gccgcatcgt gttgccgctc gccgcgcccg ggatcgccgc aaccgccatt
     3001 ttcgccttcg tggtatcctg gagcgaactt ttgttcgcga tctcgttcac gaccgagacc
     3061 agcatgcgca ctttgtcggc cgggctgctc tacatggtcg ggcagtacga ggtgcaatgg
     3121 gggccgctgt cggccggcgt catcttgagc acggtcccgg tggcggtgct gttcagcttc
     3181 ctgcagcgcc atttgatcca cgggctcacc gcgggcgcgg tgaagggtta ggcgcggttc
     3241 aaaccgtgct gcgcaggatg agttggcttt ggcacatctc cagcgggcgg atgaatgagc
     3301 aggcgcccga accaacgtcg tgttaggctc tcgccgggga cgtgggggaa gggtatgtga
     3361 cgtcgagaat tcatcacgct tgctggtagc gcggcggcct catggccgct cgcggcgcac
     3421 gcacaaaagg cgccaatccg cctcggattt ctagccagcg gtgccgctgc acagctgctg
     3481 caggctagct ttcgccgcgc tcagacgtcc tgctcgcgga atgccgcagc ccgccgctgc
     3541 gcccgcagcc acaccgcgaa ggcgatcacc acggcacccg ccagggcaaa ccaggtgatg
     3601 gcgtattgca aatgatcgtc cttgagatgg acctcgagcg cacccggttt cgggatgccg
     3661 cttgcgggca ccggctgctc caggtcgata tagaacggcg caaccggcgc accgccctcg
     3721 ccccagccca gagcgcgcgc catggccaga tgatcccgcg tgaaccacag ccgctttgcg
     3781 ggattttccg gcggcgtcag cagccccgcc gcttcgggga agcggatgta gccggttaaa
     3841 ttcacccgct ccttggtgac gagccgcgcc accgcgcgat cctgctgctc gcgatcctgc
     3901 atcgtgtttg gcacgaagcc cgcattgacc acgacggtct ctccgcctgg caggcgcgcc
     3961 ggcatgaacg cccatgtacc ggggccggaa atatcctcgc gaatccccga gccggaactg
     4021 tagaccatcg catccgggcg ggattcgtac gtcgccgcaa aactgatgcg gcggaattcg
     4081 tcatttgccg gcgtgagcgc gctccactgc gaaggcgagg gcagcggtcc aggcgttgcc
     4141 gctaggcgct cgctcaaggc cgcgatcaag gcatgcttct cgacccggcg ctgcaactgc
     4201 cacaccccga gcccaacgaa cagcgtcacc atcgcgagcg tgaacaggcc gaaaccagcg
     4261 acgccacgcc gccgcatctt tgcctcgatc atttcggctc gcgatcgatc agccggcctt
     4321 ctgcggcctt gtggtggtac tgcagcgcga tcaggagcga cttcatcgag cgcaacggca
     4381 acagcgtggt cgccaggatc agcggcagcc agagcaccgc gtgcagccag aacggcggct
     4441 gatacttgat ctcgacgatc agggcgcagc cgacgacgac ggcgccggca atcatgatga
     4501 tgaaaaccgc cggcccatcg ccagagtcga taaaggcata gtctagcccg caggcttcgc
     4561 agttcggccg caggttgagg aagccggcat acagcttgcc ccggccgcaa cgaggacatt
     4621 tgcaggcaag cccgcgcatc gcgctctcgg tcaccgtagg tgtttggtcc tgcatgcgtt
     4681 ggtgttccca aacaaaaggg cggccctgcg gccgccccgt tgtagcacag tggctctttt
     4741 gttttgacgc gtttccttta tgcgaaccgg tatcgccttc gctcggaaac gctatggcag
     4801 atcaatgcgc gccgtgggcc atggtttccg cgccgtggcc ccagacatag atgcagatga
     4861 acaggaacag ccacaccacg tcgacgaaat gccagtacca ggcggcgaat tcgaagccga
     4921 gatgctgggt cggcgagaaa tgcccgagat aggcgcggac caggcacacg gtcaggaaca
     4981 tggtgccgat cagcacatgg aacccgtgga agccggtcgc catgaagaag gtcgcgccgt
     5041 agatattgcc ggcgaaggag aacgcggcat gggcgtattc ataggcctgc acgcaggtga
     5101 agcaggcgcc aagcgcaatc gtcagcatca ggccgtattt cagcccctgg cgatcgttct
     5161 ccagcagcgc gtggtgcgcc caggtgaccg tggtgcccga ggtcaacagc aatagcgtat
     5221 tcaacagcgg cagatgccag ggatcaaagg tctcgatgcc ctttggcggc cagacgccgc
     5281 cgaacagcgc ctcgcgggtg gcgtggaccg gatcggccgg gaacagcgcc gagttgaaga
     5341 acgcccagaa ccaggcgacg aagaacatca cctcggaggc gatgaacagg atcatgccgt
     5401 agcggtggct gatctgcacc acgcgagagt gatcgccctt gtactgcgcc tcgcggatca
     5461 cgtccgtcca ccagcctgcc atggtgtaga gcacgccgat ggtgccgacc ccgaagatga
     5521 tcggcgccgc cgcgaacata tgatgcatcc aggcgattgc gccgaccgcc atgataaagg
     5581 ccgagaccgc gccgacgatc ggccacgggc tcggatcgac gaggtggtaa tcatggtgct
     5641 tgacttgcgc cgtagccatt tcggtctctc cgttagcggt gccgtggttt acccggcacg
     5701 tcttcgtccc aaaaatcaaa tcccgggatc ttttagaacc cggagatcgc cagcctcaaa
     5761 gtcttccctc gcgcttgtcc ggctcgcccg cggccagcgg cctcgctgcc ggatcgcgca
     5821 cgggatagaa agtataggaa agcgtgatgc cgttcagccc gtcattgtcg ctgtccttga
     5881 ccagcgccgg atcgacgtaa aacaccaccg gcatctcgcg tgtctcgccc gccgccatgg
     5941 tctgttcggt gaagcagaag cagttgatct tctggaaata cgccccgacg gtgagcggcg
     6001 ccacattata ggccgcctgc ccggtcgtgg cccgcgccgc ttgattggtc acggtgtaga
     6061 acacggtgac gacttcgccg atcctgacct cgatctcggt ctgctccggc tcgaatttcc
     6121 acggcaggcc ggccgcgaca ttggcatcga agcgcaccgc gatcttgcgc gccagcggtg
     6181 ccgatgacgg cgccgaggtc gcgacttgcg tggtgccgtt gaagccggtg gtgcggcaga
     6241 accagttgta gaatggcacg gccgcatagg acgcgccgac catcagcacc acgatgaggc
     6301 cgcagatcgc cgccactgcc gcatcgcgac ccaagccgcg acgagagccg cggcgtgaag
     6361 tctcctcaag gcttctggtc ggggcttgcg gcatcgtcgg ctcgttctcc atatccatca
     6421 catcggccgc actagcacgg ccggcccctt gacgagggtg actgcgaaaa acagcacggc
     6481 cagaacgccg agcgccaatg cgatcgcgat cgaacgctgg cggcggcttt tcttctgcgc
     6541 ctcggtgagg acgattccat ctggctcccg cttgtcgtcc attgcttcgc catgcgcccc
     6601 ctctaccaga tcgttggcgc gagcgcgcgg acaacgacct cgagcagcaa ggtcgcgaac
     6661 agcccaaaca gataaaggat cgagaacgca aacagctttc gcgtcgcgcg ccgcgcttcg
     6721 cttccctcgc ggcggcggta gacgctgacc gcgagcgcca gcatggcgcc gccgagaacg
     6781 agcgaggtca cgccataaac cgcgtcgaaa taaccgagcg gccacggcgc gaaggcgacc
     6841 gcgatcagca cgatcgtgta gagcaggatc tgcagccgcg tcgcatcggg gccggcgacg
     6901 acgggcagca tcggcacgcc ggcacgcgca tagtcgtcgc tgcgaaacag cgccagcgcc
     6961 cagaaatgcg gcggggtcca gaagaagatg atcaaaaaca gcaatattgg ctcgaccgac
     7021 agcgatccgg tcgccgccgc ccaggccacc accggcggca gcgcgcccgc cgcgccgccg
     7081 atcactatgt tctgcgcggt ccagcgcttc agcgcgatgg tgtagatcac gacgtagaag
     7141 aagatcgtaa acgcgagcag cgcgccggca aaccagttga ccaggatgcc gagcgtcatc
     7201 acggagaaga acgccagcgt agtgccgaat gtcagcgctt ccgcctgcgt gatacgcccg
     7261 cgcgggatcg gccgattggc ggtgcgcgac atctttgcgt cgatatcgcc ctcgtaggcc
     7321 atgttgagcg cgccggaagc gccggcgccg accgcgatgc agagaagcga ggtgaacgcc
     7381 agcaccgggt gaaaatgccc cggcgcgagc acgaggccga ccagcgcggt aaagatcacc
     7441 agcgacatca cccgtggctt caacagcgcg atgtagtcgg cgacatcggc ctcggaaatc
     7501 cggggcgaca gctcgatggc gttgcgatcg acaaccgaca agatttggct tccctaaggc
     7561 gcggcgccaa caggcgcccg acgcatttcc gattactgca cgcgcggcag cacttcgaac
     7621 tggtggaacg gcggcggcga cggcagcgtc cattccagcg tggtggcgcc ggctccccat
     7681 ggattgtccg cggcctgctg cttgcgcacg aaggcgtcga ccaggcccca gaggaagatc
     7741 agcacgccga accccgaaat gtaggagccg atcgacgaca ccagattcca ccccgcaaac
     7801 gcgtcgggat aatcgacgta acggcgcggc atgccggaga gaccgaggaa gtgctgcggg
     7861 aagaacacca ggttgacgcc gatgaaggtg aaccagaagt gcaatttccc gatggtctcc
     7921 gaatacatgt agcccgacat tttcggaaac cagtagtacc agccggcgaa gatgccgaac
     7981 accgcgccga gcgacagaac atagtggaaa tgcgcgacca cataataggt gtcctgcagc
     8041 acgcggtcga cgccggcatt ggccagcacc acgccggtga cgccgccgac ggtaaacagg
     8101 aagatgaagc cgatcgccca cagcatcggc gtcttgaact cgatcgagcc gccccacatc
     8161 gtcgcgatcc aggagaagat ttttacgccg gtcggcaccg cgatcaccat ggtggcggcg
     8221 acgaaatagg cctgcgtcgc cga
//
LOCUS       DATNOI010000517        13592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2116815,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000517 DATNOI010000000
VERSION     DATNOI010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13592
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116815"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..770
                     /locus_tag="VN968_20810"
     CDS             15..770
                     /locus_tag="VN968_20810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31712.1"
                     /translation="MSGTLGIKAARIAIVLGVFALWETLSRTGIVNPRLLPSASDTFA
                     MLGELLQRASVRNDLVVTASEVLTAFVLAVPVGTLVGFLIAENRYFAEVTKPLLFFAF
                     SIPKSIFLPMFILVFGVGFSEKVGFGFFSTIFIVIMSTTTAVESVKVEHLTVARSYGA
                     TAVQTAFRIYLPSMLPVLLEALRISMIFNLTGVILAEMYASRDGIGHQIATWGENFQM
                     KQLLAGVLMIGAIAMAFNELVRWVEMRCSHWRT"
     gene            749..1555
                     /locus_tag="VN968_20815"
     CDS             749..1555
                     /locus_tag="VN968_20815"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31713.1"
                     /translation="MQPLANVTPLKPAARAGSVQVTGVGQIYRTSGQDVVALSDVSIE
                     VKPGRFVVLVGPSGCGKSSLLMMMAGLRPQTSGTILINGAPIDVPDPDRVGVVFQEPS
                     LFPWLTAEENVEFPLTLRGVARKARRIKAEEALQLVGLDGFGKRHPHELSGGMKQRVS
                     IARGLVQDPPVLLMDEPFAALDEQTRMTMGDELLRIWTATGKTIIFVTHSLTEAVYLA
                     DEVVVMSARPGRIVDRLDVALPRPRTFEMLSGDAFGSLRDRIWRHIRKSA"
     gene            1566..2372
                     /locus_tag="VN968_20820"
     CDS             1566..2372
                     /locus_tag="VN968_20820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31714.1"
                     /translation="MSSTTLRRLIIMALIVLWEVLPRIGAIPELFLPSLSSTLQAGWS
                     DAGEYGHALMVTLYEVAVAMVFACGGGILGGAVVGSLPRPRVLIMPMVSSLYAVPLVI
                     LYPVFTVWLGIGSESKIAFASVYGFLPTMLATAAGIQTIDPQLLLAARSMGATLRQRI
                     TRVIIPAAIPTVLSGLRVGGALVIVGVVVSEMLISSAGIGYLISRYRTILESAHVFAG
                     VLLVLVMAVAFNALIKFVERRAAVWQTGTRAGRDTIAREIPAAVLQPTNS"
     gene            complement(2391..3431)
                     /locus_tag="VN968_20825"
     CDS             complement(2391..3431)
                     /locus_tag="VN968_20825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXL31715.1"
                     /translation="MALPKHVLPTTVVGSYPQPDWLVDREMLSKSVPRTRMTQMWRIP
                     QAYLEQAQDDATILAIRDLEHAGIDIVTDGEIRRESYSNRFATALEGIDTDNPAIITT
                     RSGQQTPVPRVVGNVVRTGPVELRDMEFLRRNTDRAAKITLPGPFTMSQQAKNEFYKD
                     DEELAMALAAAVNAEALELQKAGADVIQLDEPWVRNDPAAARRYAVKAINRALQGITV
                     PTVVHICFGYAAVVPGDTKPSGYSFLAELADSTAEQISIEAAQPKLDLGVLADLSSKK
                     ILLGVIDLGNPEIETAAVIADRIRHGLNYVSADRLIPAPDCGMKYMPRHVAFGKLKAM
                     SEAAAIVRKEIS"
     gene            complement(3636..4289)
                     /locus_tag="VN968_20830"
     CDS             complement(3636..4289)
                     /locus_tag="VN968_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017277705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXL31716.1"
                     /translation="MTITITAFERSPDGGKGLARDMRVRWALEEVGQPYEVRLVSFRA
                     MKKPAHLALHPFGQIPTYEEGDLGLFETGAIVFHIAQRHAGLLPDDADARARAITWMF
                     AALSTVEPPILELGTARLLEGDKSWSAQRMPLVEDRVRNRLGQLSRRLGDADWLDGAF
                     SAGDLMMVSVLLRLKASGLLDEYPNLSAYVARGEARAAYKRAFDAQLAVNTGKQPTG"
     gene            complement(4314..4580)
                     /locus_tag="VN968_20835"
     CDS             complement(4314..4580)
                     /locus_tag="VN968_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31717.1"
                     /translation="MSANVRSSNRSFGVQALSRINRSIGNKLATSVRVEIHRHELKYS
                     LVLIDASAALKDLETRTLLEQQTMQILGSSPAAFALTACQPRSR"
     gene            complement(4591..5385)
                     /locus_tag="VN968_20840"
     CDS             complement(4591..5385)
                     /locus_tag="VN968_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL31718.1"
                     /translation="MDTYATKTPLVDMRNISIAFGGIKAVDDVSVQLHEGEVVGLLGH
                     NGAGKSTLIKILSGAYPADSGEIWVNGEKASIASPRDAKTYGIETIYQTLALADNVDA
                     AANIFLGREILTGWGTLDDAAMESQTRRVMARLNPHFRRFKDPVRALSGGQRQSVAIA
                     RAIHFDARILIMDEPTAALGPAETKQVGDLTLSLKQEGIGIFLISHDVHDVFDLADRI
                     YVMKNGRLVGSARVSDVTKDEVLGMIILGKCPPGGLPGPGAAPRER"
     gene            complement(5397..6650)
                     /locus_tag="VN968_20845"
     CDS             complement(5397..6650)
                     /locus_tag="VN968_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL31719.1"
                     /translation="MGNLFRKLELDLRLVGMLAALAIIWVGFDTLTGGEFLTPRNLWN
                     LSVQSSSVAVMATGMVLVIVTRNIDLSVGSILGFTGMIIGVTQAAILPQLWGFDHPLT
                     WVVAVIVGLLVGTAIGALQGAVIAYLGVSSFIVSLGGLLIWRGAAWWVTTGQTVAPLD
                     SRFKPIGGGIEGAIGANASWAIGIVAVALAIAILITARRRRQGFGFTVRPPWAEAVIA
                     ALISIAILGAVLVVNSYPLPIGVVRRLQAAAGAPEDTPLPEIAHGFAMPVLIAIAVGL
                     AMTFLTNRTRFGRYVFAIGGNPEAAELSGIYTKRVLTRVFALMGLLAGISACISTARL
                     QSATNATGTLDELYTIASAVIGGTSLTGGTGTIYGAMLGAVVMQSLQSGMVLLGVDAP
                     LQNIVVGLVLVAAVWIDTIYRRHLK"
     gene            complement(6925..7968)
                     /gene="xylF"
                     /locus_tag="VN968_20850"
     CDS             complement(6925..7968)
                     /gene="xylF"
                     /locus_tag="VN968_20850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004671310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_function: GO:0033222 - xylose binding [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL31720.1"
                     /translation="MKIIPTVFAASALALLAANGALAAGKPVIGVSWSNFQEERWKTD
                     EAAIKAAIEKAGGSYISADAQSNPGKQLTDIESLITKGANALIVLAQDSDAIRPAIEK
                     ATQEGIPVVGYDRLIEIPSAFYITFDNKEVGRMQARAVHAAKPKGNYAFIKGSSTDPN
                     ANFLFAGQIEVLKAAIDKGDIKNVGEAYTDQWLPANAQKNMEQILTKNNNKVDAVVAS
                     NDGTAGGAVAALSAQGLAGSVPVSGQDGDKAALNRVALGTQTVSVWKDARELGKNAAE
                     IAIALAGGKKLSDIPGSTTFSDGPKKQKMTARLLKPVPITKDNLSVVIDAGWITKDAV
                     CQGVKAGSVSVCK"
     gene            complement(8078..9400)
                     /gene="xylA"
                     /locus_tag="VN968_20855"
     CDS             complement(8078..9400)
                     /gene="xylA"
                     /locus_tag="VN968_20855"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HXL31721.1"
                     /translation="MSSSPRFFETALPVSFGGSSAEAPHAFRWYDKNRIVAGKRLEDH
                     LRFAVCYWHSFCWPGSDPFGGETFLRSWHHGGDALDLAKAKADVAFELFRLLDVPFFT
                     FHDVDAAPEGASLKESIYNLDVMAEIFLEKMAASKVRLLWGTANLFSNRRYMAGAATN
                     PDPDVFAYAASQVRAALETTHRLGGQNYVLWGGREGYETLLNTDMRQELDQLGRFVSM
                     VVEHKHKIGFKGPILIEPKPKEPTKHQYDFDVATCYGFLERYGLTKEVKLNLEQNHAI
                     LAGHSFQHEIALANAFGVFGSLDINRGDDLLGWDTDQFAMNVPEMALCFHELLKGGGF
                     TTGGLNFDAKIRRQSIDPDDLIHAHVGSMDACARAFIAAAEMLDAGALTGQIAERYAG
                     WQAPQARAILAGNQSLAEVADRALMEGLNPQPRSGRQEYLESLVNRFV"
     gene            complement(9400..10857)
                     /gene="xylB"
                     /locus_tag="VN968_20860"
     CDS             complement(9400..10857)
                     /gene="xylB"
                     /locus_tag="VN968_20860"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HXL31722.1"
                     /translation="MYLGLDIGTSSVKAVLVDAAGSVKVVASRALDVSHPNPLWSEQN
                     PDSWVEATIGAVDELASTHPVAVAAVRGVGLSGQMHGATLLDAAGTPLRPAILWNDGR
                     SHVECRELEARLPALHSIAGNLAMPGFTAPKLLWVANHEPEIFRRTAKVLLPKAYVRF
                     RLTGTMVEDMSDAAGTLWLDVGRRRWSEEILAACRMSLAQMPSLVEGSESSGQLTADL
                     TTRWGMKPGVVVAGGAGDCAASAIGLGAIAPGQAFLSLGTSGVLFAVTDQFAPNAISA
                     VHAFCHALPGIWHQMGVMLSAAASLAWLADVLRAHEADLLEPLGTAVDSPSPVKFLPY
                     LSGERTPLNDAKASGAFLGLRADTSRSHIVQAVLEGVAFAARDNLAALAAAGTHLSEV
                     DLVGGGSRSPLWTQIVADVLGLPVHRVEEGEVGAALGAARLGRLADTGEAPAVVCTRP
                     CRLATCLPRPAQVAAYEESYAQWSKLYPALKEVSR"
     gene            complement(10969..12012)
                     /locus_tag="VN968_20865"
     CDS             complement(10969..12012)
                     /locus_tag="VN968_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXL31723.1"
                     /translation="MSASTPNPTLTLKDIAREAGVSLATVDRVLHKRPGVRPGTVKRV
                     KDTIERHEFRPFAAAADLARGRTNRFAVVMTAGSNLFMQQILSHLREMNGWLSARRIV
                     VEVVTTDVFNPEVLARTLDSLHGQFDGLAVVALDHPSVRAAIDDLIDSGTPVVTLVSD
                     VPSSRRQHYVGIENIAAGRTAGTLMGRLIGPRNGKVAIVAGSQGLRDHAERIFGFNQV
                     LGSDFPKLDVLLPVEGRDEDDISENLTVRLLSAHPDMVGLYNVGAGAQGVAKAVTDSG
                     RANEIVFIGHDLTSFTRRLLLRGVMDAAIVQDPGHEARSVVRVLLALVRQEPILVEQE
                     KIRIDIIMRDNMP"
     gene            complement(12129..12311)
                     /locus_tag="VN968_20870"
     CDS             complement(12129..12311)
                     /locus_tag="VN968_20870"
                     /inference="COORDINATES: protein motif:HMM:NF017128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 family protein"
                     /protein_id="HXL31724.1"
                     /translation="MAQIGTFTRAEDGSYSGTIKTLSLSIKARFLLAEPSENEKAPNL
                     RGMAGNVEIGAAWQPT"
     gene            complement(12849..>13592)
                     /locus_tag="VN968_20875"
     CDS             complement(12849..>13592)
                     /locus_tag="VN968_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HXL31725.1"
                     /translation="NFPVSQWAGLAHFNITKEVQISAGVYDANPNYLTTSDMGVYLAP
                     GIPASSPASGVLVPVELTWRPGGHLYGTWRLGGWYDSASTIDGGLPGIITTIPGVGGV
                     PDQNLGDQRGRYGFYESILQRLTDEGGPAKGWYIYLNTSVADHRTSFQDYQVSLELKH
                     TGTFASRPEDEVGVAVGTTHVNSAALSPNAGGNEVPLEAWYGLQATGWLNLKFDAQYV
                     INPGGRGFNAAGLKTDNAWVFGLRTVVKF"
BASE COUNT         2521 a         4182 c         4286 g         2603 t
ORIGIN      
        1 ctgaggcgcg cgcgatgtcc ggcacgctcg gaataaaggc ggcgcgcatt gcgatcgtgt
       61 tgggcgtgtt cgcgctgtgg gaaacgctgt cgcggaccgg gatcgtcaat ccgcggctgc
      121 ttccctcggc gtccgatacc ttcgccatgc ttggcgagct gttgcagcgc gcgagcgtgc
      181 gaaacgacct ggttgtgacg gcgagcgaag ttttgaccgc ctttgttctc gccgtgcccg
      241 tcggcacgct ggtcggcttt ctgatcgcgg agaaccgtta cttcgccgag gtgacaaaac
      301 cgctgctgtt cttcgccttc agtattccaa agtcgatctt tctgccgatg ttcatcctgg
      361 tattcggggt tggtttttcg gagaaagtcg gcttcggttt cttctccacc atcttcattg
      421 tcatcatgtc gacgacgacc gcggtcgaat ccgtgaaggt cgagcatctg acggtggcgc
      481 gctcttatgg ggcgacggcg gtgcagacgg catttcggat ttatctgccg agcatgttgc
      541 cggtgctgct ggaagcgctg cggatttcca tgatcttcaa tctcactggc gttattctcg
      601 cggagatgta cgcctcgcgg gacggcatcg gccaccagat cgcgacctgg ggagaaaact
      661 tccagatgaa gcaattgctg gccggcgttc tgatgatcgg cgcgatcgcc atggccttca
      721 acgaactcgt caggtgggtg gaaatgcgat gcagccattg gcgaacgtaa ccccgctcaa
      781 gccggcggcg cgcgcaggct cagtccaggt caccggcgtc ggccagatct acaggacctc
      841 cggccaggac gtggtggcgc tgtccgacgt atcgatcgag gtcaagcccg gccgttttgt
      901 cgtgctggtc ggtccgagcg gctgcggaaa gtcgtcgctg ctgatgatga tggccgggtt
      961 gcggccgcaa acctcgggta cgattttgat caacggcgcg ccgattgacg ttcccgaccc
     1021 ggatcgggtc ggtgtggtgt tccaggagcc gagcctgttt ccgtggctga ccgccgaaga
     1081 gaatgtcgaa tttccactga cgctgcgcgg cgttgccagg aaagcgcgcc gcatcaaggc
     1141 ggaagaagcc ttgcagctcg tcggtctcga cggcttcggc aaacgtcacc cgcatgaatt
     1201 gtccggcggc atgaagcagc gggtctcgat cgcacgcggc ctggtgcagg atccaccggt
     1261 attgctgatg gacgagccgt tcgccgcact cgacgagcaa acccgcatga ccatgggcga
     1321 tgaattgctg cgaatctgga cggcgaccgg caagaccatc atcttcgtga cccacagcct
     1381 gaccgaagcc gtctatctcg cggacgaggt ggtggtgatg tcggcgcgtc ccggccgcat
     1441 cgtggaccgg ctggacgtcg cgctgccgcg cccgcgcacc ttcgagatgc tgagcggcga
     1501 cgcgttcggt agcttgcgcg accgcatctg gcgccatatc cgcaagtcgg cgtgagggaa
     1561 tggtgatgtc gtcaaccacc ctgcgccgcc ttatcatcat ggccctcatc gtattgtggg
     1621 aagttctgcc gcgcatcggc gccattcctg aactgttcct gccgtccttg tcgtcgacgc
     1681 tgcaggccgg ctggagcgac gccggtgaat acggccacgc gttgatggtg acgttgtacg
     1741 aggtcgccgt cgccatggtg tttgcatgcg gcggcggaat cttgggcggc gcggtggtcg
     1801 gcagcctgcc gcggccgcgc gtgctgatca tgccgatggt gtccagcctc tacgccgttc
     1861 cgctggtgat tctctatccg gtattcacgg tatggctcgg gatcggttcg gaatccaaga
     1921 tcgcctttgc cagcgtatac ggctttctgc cgaccatgct ggcgaccgcc gccggcattc
     1981 agaccatcga tccgcaattg ctgctggcgg cgcgcagcat gggcgcgacg ctccgtcagc
     2041 gcatcacccg cgtcattatt ccggcggcga ttcccaccgt gctttcaggc ctgcgggtcg
     2101 gcggcgcgct ggtgatcgtc ggtgtcgtcg tatcggagat gctgatctcg tcggcgggca
     2161 tcggctatct gatctcgcgt taccggacga tcctggaaag cgcccatgtg ttcgccggcg
     2221 tgctgctggt gctggtgatg gcggtcgcct tcaacgccct gatcaagttc gtggaacgac
     2281 gcgccgccgt ctggcagacc ggcacccgcg cggggcggga taccatcgcg cgagaaattc
     2341 ccgccgctgt gctgcagccg acgaattcct gacacgctgc tcgtcaggta tcagctgatt
     2401 tccttgcgca cgatggctgc ggcctcactc atcgccttca gcttcccgaa cgcgacgtgg
     2461 cgcggcatgt atttcatgcc gcagtccggc gccggaatca gccggtccgc ggagacgtag
     2521 ttgaggccgt ggcggattcg atcggcgatg acggctgcgg tctcgatctc gggattgccg
     2581 aggtcgatca cgccgagcag gatctttttc gaggacagat cggcgagcac gccgagatcg
     2641 agtttgggct gcgcggcctc gatcgagatt tgctcggcgg tggaatccgc aagctccgcc
     2701 agaaaggaat agcccgacgg cttggtatcg ccgggcacga ccgcggcgta gccgaagcag
     2761 atgtggacga ccgtcggcac cgtgatgccc tgcagcgcgc gattgatggc tttcacggcg
     2821 tagcgcctgg cggcggccgg atcgtttcgc acccagggtt cgtcgagctg gatcacgtcg
     2881 gcaccggcct tctgcagctc cagcgcttcg gcgttcaccg ccgccgccag ggccatcgcg
     2941 agctcttcgt cgtccttgta gaattcgttc ttggcctgct ggctcatggt gaatgggccg
     3001 ggcagggtga tttttgcggc gcgatcggtg ttgcggcgaa ggaattccat gtcccgcagt
     3061 tcgaccggac cggtacggac aacgttgccc accacgcgcg gaaccggcgt ttgctgtccc
     3121 gaccgcgtgg tgatgatggc aggattgtcg gtgtctatcc cctccagcgc ggtcgcaaaa
     3181 cggttggagt agctttcccg cctgatctcg ccgtcggtca cgatatcgat gccggcgtgt
     3241 tccaggtcgc ggatggcgag gatcgtcgca tcgtcctgcg cctgctcgag ataggcctgc
     3301 ggaatccgcc acatctgcgt catccgggtc cgcggaacgg attttgacag catctcgcga
     3361 tccaccagcc agtcgggctg cggataactt ccaacgacgg tggtcggcaa cacgtgcttg
     3421 ggtagcgcca atatctcaac tccctggggg caggctgatt gttcttgtac cgaatgtaag
     3481 ggttatctgc cgtgaatgta aacgctctcg aaattcggaa gcgtccttgc tgctccgcgg
     3541 gtctcggcca tccttacctg actcccacct aaggtcagga cccataaact gaacgaaaac
     3601 tcggatgacg acttcgagcc caggacggac ctcaatcagc cggtcggttg cttgccggtg
     3661 ttaaccgcca attgagcgtc gaaggcacgc ttgtaggcgg cccgcgcttc gccgcgggcc
     3721 acataggcgg agaggttcgg atattcgtcc agcaaacccg acgctttcaa cctgagcagc
     3781 accgacacca tcatcaggtc gcccgcgctg aacgcgccat cgagccagtc ggcatcgcca
     3841 aggcgacgag aaagttggcc cagccggtta cggacgcgat cctcgacgag aggcatgcgt
     3901 tgcgcggacc aggacttgtc gccctcaagg agcctggcag ttccgagttc aaggatcggc
     3961 ggctccaccg tgctgagcgc ggcgaacatc catgtgatcg cgcgcgcccg ggcatcggca
     4021 tcgtccggca gcaggcccgc atggcgctgc gcgatgtgga acacgatcgc ccccgtctcg
     4081 aacaacccga gatcgccttc ttcgtaggtc ggaatctgcc cgaaaggatg aagcgccaga
     4141 tgtgcgggtt tcttcatcgc cctgaacgaa acaagacgaa cctcgtaagg ttggcccact
     4201 tcctccagcg cccagcgaac gcgcatatca cgcgccagtc ccttgccgcc atcgggcgac
     4261 cgttcaaagg cggtaatcgt gatggtcatc gtgagacttc tccctggcca aaatcatcgg
     4321 ctgcggggct ggcaagctgt caaggcgaag gccgccggcg aactgcccaa gatctgcatt
     4381 gtctgctgtt ccagcagcgt cctggtttcc aggtctttca gcgccgccga cgcgtcgatg
     4441 agcacaagag agtacttcag ctcatgacga tgtatttcca cgcgcacgct cgtcgcaagc
     4501 ttattcccga tcgaacggtt gatccgcgaa agcgcctgga ccccaaacga ccgattagag
     4561 gagcggacgt tcgccgacac ggcggtgggc ctagcgctct cttggcgccg cgccgggacc
     4621 cggcaggccc cccggcggac atttgccgag aatgatcatg cccaggacct cgtccttggt
     4681 gacatcgctg acacgggcag agccgaccaa ccgaccattc ttcatgacat agatgcggtc
     4741 ggcgaggtcg aagacgtcat ggacatcatg gctgatgagg aagatgccga tgccttcctg
     4801 tttcagcgag agagtgagat cgccgacctg cttggtctca gcggggccaa gcgccgccgt
     4861 cggttcgtcc atgatcagga tgcgcgcatc aaaatggatg gcgcgggcga tcgcaacaga
     4921 ctgacgctgg cctccagaaa gtgcgcggac cggatccttg aagcggcgaa aatgcgggtt
     4981 gagccgggcc atgaccctgc gggtctggct ttccatcgcg gcgtcgtcga gggtacccca
     5041 gccggtaagg atctcgcggc caaggaaaat attggcggcg gcatcgacgt tgtcggccag
     5101 cgccagggtt tggtagatgg tctcgatgcc atatgtcttg gcgtcacggg gagaggcgat
     5161 cgaggccttt tcgccattga cccagatttc gccactgtcg gccggatagg cgccggaaag
     5221 gatcttgatc agagtcgatt tgccggcgcc attatggccg aggagcccga ccacttcgcc
     5281 ctcatgcagt tgcaccgaga cgtcgtccac cgccttgatg ccgccaaagg cgatggagat
     5341 gttccgcata tcgacaagcg gcgttttggt cgcgtaggtg tccatggagg tctccctcac
     5401 ttcaggtggc ggcgatagat ggtatcgatc cagacggcgg cgaccagcac cagtccgacc
     5461 acgatgttct gcagcggcgc gtcaacgccg agcagcacca tgccggattg cagcgactgc
     5521 atcaccaccg cgccgagcat ggcgccgtag atcgtgccgg tgccgccggt tagcgaggtg
     5581 ccaccaatga cggcggaggc gatcgtgtag agttcgtcga gcgtgccggt ggcgttggtc
     5641 gccgattgca ggcgggcggt ggaaatgcag gcagagatgc cggcgagaag ccccatcaga
     5701 gcgaagactc tggtgaggac acgcttggta tagatgccgg agagttcggc ggcttccgga
     5761 ttgccgccga tggcgaaaac atagcggccg aaacgggtgc gattggtgag gaaggtcatg
     5821 gccaggccga cggctatcgc gatcaacacg ggcatggcga agccgtgggc gatttcgggc
     5881 aagggtgtat cctcgggggc tccggcggcg gcctgcagtc ggcgcaccac gccgatcggt
     5941 agcggatagg aattgaccac cagcactgcg ccgaggatcg cgatcgaaat gagcgccgcg
     6001 atcaccgctt cggcccaggg cggtcggacc gtgaagccaa agccctggcg gcggcggcgc
     6061 gcggtgatga ggatggcgat ggccaaagct accgctacaa tgccgatcgc ccagctggcg
     6121 ttggccccga tggcaccttc gatgccgccg ccgattggct tgaagcggct gtcaagcggg
     6181 gcgactgtct ggcccgtagt cacccaccaa gccgcaccgc gccagatcag caggccgccc
     6241 agcgacacga tgaaggagga gacgccgaga taggcgatga ctgcgccttg cagcgcgcca
     6301 atagctgtgc cgaccagcag cccgacgatc actgcgacga cccaggtgag gggatgatca
     6361 aaaccccaga gctgcggcaa aatcgcagcc tgcgtcacgc cgatgatcat tccagtgaag
     6421 ccgaggatcg aaccgacgga gagatcaatg ttgcgggtga caatgaccag caccatgccc
     6481 gtcgccatga cggcaaccga ggaggactga accgaaaggt tccatagatt gcgcggggtc
     6541 agaaattcgc cgccggtgag agtgtcaaag ccgacccaga tgatagctag tgcggccagc
     6601 atgccgacca gcctcagatc gagttcgagc ttgcggaaga ggttacccat aacgggggga
     6661 ctccggtgga gaataagtgg cgttatcggg gccggggccg gccccttggc catcgtcccg
     6721 ggcttggccc ggatccaaat cagcctacgg tcagtcgttt cccgtcgatc atcgcgcgtc
     6781 cccgccacag ccaggggcta gcgccggtct cgccgaactg gatccagctt cgagcgcaaa
     6841 ggaaagcacg cgcaacgacg cgtccgcgat cactgcgaaa gagggggaga tgccggtcgt
     6901 ccggcacctc ccgctcgtat cacatcactt gcagaccgaa accgatccgg ccttgacgcc
     6961 ctggcagacg gcgtccttgg tgatccagcc ggcgtcgatt acgacggaca ggttatcctt
     7021 ggtaatcggg accggcttca ggagccgggc ggtcatcttc tgcttttttg ggccgtcgga
     7081 gaacgtggtc gagccgggga tgtccgaaag cttcttgcca ccggccagcg cgatggcaat
     7141 ttcagctgcg ttcttgccga gttcgcgggc atccttccac accgatacgg tctgggtgcc
     7201 gagtgccacg cggttgagcg cggccttgtc accatcctgt ccggagaccg gaacggaacc
     7261 agccaggcct tgtgccgaaa gagcggcaac agcgccaccg gcggtgccat cattggaggc
     7321 gacgaccgca tcgaccttgt tgttattctt ggtcaggatc tgttccatgt tcttctgggc
     7381 gttggcgggc agccactggt cagtataggc ctcgccaacg ttcttgatgt cacccttgtc
     7441 gatagcagcc ttcaaaacct cgatctggcc ggcgaacagg aagttggcgt tcggatcggt
     7501 cgacgagccc ttgatgaagg cgtaattgcc cttgggcttg gcggcatgca cggcgcgggc
     7561 ctgcatgcgg cccacttcct tgttgtcgaa ggtaatgtag aaggcgctcg ggatctcgat
     7621 caggcggtca taaccgacga ccgggatgcc ttcctgggtg gctttttcga tcgcgggacg
     7681 gatggcgtcg gaatcctggg caaggacgat cagggcgttg gcgcccttgg tgatcaggct
     7741 ttcgatatcg gtcagctgct tgcccgggtt cgactgagcg tcggcggaga tatagctgcc
     7801 accggccttt tcgatcgcgg ccttgatcgc tgcctcgtcg gtcttccagc gctcttcctg
     7861 aaaattcgac caggaaacac cgataaccgg tttgcctgca gccaacgcgc cgttggcggc
     7921 cagaagcgcg agcgcgctcg cggcgaaaac agtgggaatg atcttcatgg tttttcctcc
     7981 gtgtttggcc catttgggcg gttggttaga gtagaacgct gtctcatcgc tcttgatagg
     8041 cgacttgaag ggtttcgatc cggggtagtt gcccgcttca gacgaaacgg ttgacaaggg
     8101 actccaggta ttcctggcga cccgaacgcg gttgaggatt gaggccttcc atcagggccc
     8161 tgtcggcgac ttcagccaaa gattgattgc cagcaaggat cgctcgtgct tggggggcct
     8221 gccagcccgc gtagcgctcg gcaatctggc cggttagcgc gccagcatcg agcatctccg
     8281 cggcggcgat aaaggcgcgc gcgcaggcat ccattgatcc gacgtgggca tggatcagat
     8341 cgtccggatc gatcgactgc cggcgaatct tggcgtcgaa attgagcccg ccggtcgtga
     8401 acccgccacc cttcagcagc tcatggaaac agagcgccat ctccggtacg ttcatcgcga
     8461 actggtcagt gtcccagccc agaaggtcat cgccgcgatt gatgtcgagc gacccgaaga
     8521 cgccaaatgc attggcaagc gcgatctcgt gctggaatga atggccggcg agaatcgcgt
     8581 ggttctgttc gaggttgagc ttgacttcct tggtcaggcc atagcgctcc aggaacccgt
     8641 agcaggtcgc aacgtcgaaa tcatattggt gcttcgtcgg ttccttcggc ttcggctcga
     8701 tcaggatcgg ccccttgaac ccgatcttgt gcttgtgctc caccaccatg ctgacaaagc
     8761 ggcccagttg gtccagttcc tgtctcatgt cggtattgag caacgtctcg taaccttcac
     8821 ggccgcccca caagacatag ttctgcccgc caagccggtg cgtcgtctcc agcgcggcgc
     8881 gcacctgact cgccgcatac gcgaacacat ccggatccgg gttcgtcgcc gcgcccgcca
     8941 tatagcggcg gttcgagaag aggttcgctg ttccccagag gagccgcacc ttcgacgccg
     9001 ccatcttttc caggaagatc tcggccatga cgtcgagatt gtagatcgac tccttcaggc
     9061 ttgcgccctc cggcgccgca tcgacgtcgt ggaaggtaaa gaacggcacg tcgagcaggc
     9121 ggaagagttc gaaggcgacg tcagccttgg ccttggcgag gtcaagcgcg tctccgccat
     9181 ggtgccagga tctgaggaat gtctcgccac caaaggggtc actgcccggc cagcagaagg
     9241 aatgccagta acagacggca aagcgcaggt gatcttcaag ccgcttgccc gcaacgatac
     9301 ggttcttgtc ataccagcga aacgcatgcg gggcctcggc ggaagacccg ccaaagctga
     9361 ccggcaaggc cgtctcgaag aaacgagggg atgagctgat caacgggata cctccttcag
     9421 ggcgggataa agcttcgacc actgcgcgta ggactcctcg taagccgcga cttgcgcagg
     9481 ccggggcaga caggtcgcca gccggcatgg gcgggtgcag acaacggccg gcgcctcacc
     9541 agtatctgcc aatcgcccga gccgagcggc gcccaatgcc gcgcccactt cgccttcctc
     9601 gacgcggtgg accggcagac ccaggacgtc cgcaacgatc tgcgtccaga gcggcgaccg
     9661 cgagccaccg ccgaccagat cgacttcgct gagatgcgtg cccgcggctg ccaaggccgc
     9721 aagattgtca cgggcggcga aggcaacgcc ttccagaacg gcctgtacga tgtgggatcg
     9781 gctcgtatcg gctcggagcc cgagaaaggc gccgctcgcc ttggcgtcat tcaagggcgt
     9841 gcgttcaccc gaaagataag ggaggaactt gacggggctc ggggaatcga cggcggtccc
     9901 gagcggttcc agcaggtcgg cctcgtgagc tctcagcacg tcggccagcc aggcaagcga
     9961 cgccgcggcc gatagcatga cccccatctg atgccagatc cccggaagcg catggcagaa
    10021 agcatgcacg gccgagatcg cgttgggtgc aaactgatcg gtgacggcaa aaagcacgcc
    10081 cgacgtgccg agagagagaa acgcctggcc cggcgcgatc gccccaaggc cgatggcgct
    10141 cgccgcgcaa tctcccgcgc cgcccgccac gaccacgcct ggcttcatac cccatcgtgt
    10201 cgtcaggtcg gcggtcagct gtccgctcga ctcgctgcct tcgacaaggc tcggcatctg
    10261 tgccagcgac atgcgacacg ctgccagaat ctcttccgac catcgccgcc ggccgacatc
    10321 aagccagagc gtgccggcag cgtcagacat gtcctcgacc atggtgcctg tcaggcgaaa
    10381 gcgaacatag gcctttggca acagcacttt cgccgtccgc cgaaagatct cgggttcgtg
    10441 attggcaacc caaaggagtt tgggtgcggt gaagccgggc atggccagat tgccggcaat
    10501 cgaatggagc gccggcaggc gtgcttcaag ctcgcgacat tcaacgtggg agcgaccgtc
    10561 attccacagg atcgccgggc gcaggggtgt accagccgca tcgagcaaag tcgcgccatg
    10621 catctgaccg gagagaccga cacctcggac ggccgcgacg gcgactggat gggtcgatgc
    10681 cagttcatca accgctccga tggtggcttc gacccaagag tccggattct gctccgacca
    10741 cagcggattg gggtgcgata catcgagggc gcgcgaggca accaccttaa cgctgcctgc
    10801 cgcgtcgacc agcacagctt tgacgctgga agtgccgata tcgagcccaa gatacacgcc
    10861 cgtcatcctc cccggtgatc cggccgacgt ttgaacacgc cttgtctggc ctggatccaa
    10921 cgctccactg cgcatcgact cgaacagccc tggccgcgtg gttctcggct agggcatgtt
    10981 gtcccgcatg atgatgtcga tgcggatttt ctcctgttcg accagaatgg gttcctgccg
    11041 caccaatgcc agcaacacgc ggacgaccga tcgagcctca tggccgggat cctgaacgat
    11101 cgcggcatcc atgacgccgc ggagcaggag gcggcgggtg aaacttgtca gatcgtggcc
    11161 gatgaagacg atctcgttcg cccgtccgct gtcggtaacg gccttggcaa cgccctgcgc
    11221 accggcacca acattgtaga gtccaaccat atccgggtga gcgcttagaa gcctcactgt
    11281 gaggttttca ctgatgtcat cttcatcacg cccctcaacg gggagtaaaa cgtcgagctt
    11341 tggaaagtcg gaaccaagaa cctgattgaa tccgaaaatc cgttcggcgt ggtcacgcag
    11401 cccttgtgag cctgcgacaa tcgcgacttt gccgttgcgc gggccgatga gacgacccat
    11461 gagcgtgccg gcggtacgtc ctgcggcgat attctcaatc cccacatagt gttgacgccg
    11521 tgacgacgga acatcggaga ccagagtcac cacaggggtg ccactgtcga tcagatcgtc
    11581 gatcgccgcc cgaacgctcg ggtgatcaag tgccacgacc gcgaggccat caaactggcc
    11641 gtggagagaa tccaacgttc gggcaaggac ttccggattg aaaacatccg tcgtaaccac
    11701 ttcgacgacg atccgcctgg ccgacaacca tccattcatc tccctgagat ggctgaggat
    11761 ttgttgcata aagaggttcg aaccggcggt catcacgacg gcaaatctgt tggttcgccc
    11821 gcgcgccaaa tcggccgccg cggcaaaggg gcggaactcg tgccgctcga tagtatcttt
    11881 tacccgcttg actgtaccgg gtctgactcc cggccgtttg tgcaatactc gatcaacggt
    11941 cgccagactc acgcccgctt cgcgggcgat gtccttcagt gtcagagttg gattgggcgt
    12001 ggatgcgctc atggcatgga cgcgtagccg aaataaaaga ggtgcgcaac tcagattttg
    12061 aggtgcggca atcgtacctt tgggaaagca gctcaatgcg agcccgtgcc gtcgctgccg
    12121 cgcggtcgtc aggtcggctg ccacgcggca cctatctcga cgttgccggc cattccgcgc
    12181 aggttcggag ccttctcgtt ctcggacggt tcggccagaa ggaagcgcgc cttgatgctg
    12241 agcgagagcg tcttgatggt gccgctgtag gatccatctt cggcgcgggt gaaggtgcca
    12301 atctgagcca tggtgatctc ctgtcgctgt atccaagccc gcgaccatcg cggccttgat
    12361 ggcgatcttt gatcgccgca cgcccgccga atttaccatc ccggtcttga tgaccgtgga
    12421 cttcgcgttc gcgatcagta gctcgctcgt ccggccgggt aggcctcgtt atccggtgtt
    12481 tgtcgattgg gccgcggctt tgctccacgc tgtcttcaga ccccacctca cgatgacgcc
    12541 cttgcgcttc gctaatcctt gcaccgccat cagactggct agaggacttc cacctccaag
    12601 ctgtcgttca tactcggcac accgcacgat ggcgcggccc gccgccgcgc ctgagcggtc
    12661 cgtggcgatc gaggttcgca tggatggtgc tcttggcggg acccctggca tcgccgtcga
    12721 ggagcccgac cagcagagct tgcggatctt gcgtgcgccc tagtggagct tctcggcatg
    12781 cccgcatacg agcatgcact cacctttcgc gtcagagtcg ttcaatatct tgaccctgac
    12841 cggtcgcatc agaacttgac cacggtgcgc aggccaaaaa cccaggcatt gtcggtcttc
    12901 aagccggcgg cattaaaccc gcgcccgcca ggattgatca catactgggc gtcgaacttg
    12961 aggttcaacc agccggtcgc ctgcaacccg taccaagctt cgagcggaac ttcgttaccg
    13021 ccggcattcg ggctgagggc ggccgaattc acatgggtcg tgcccaccgc gacgccgact
    13081 tcgtcctcgg ggcgcgaggc gaaggttccg gtgtgcttga gttccaagga gacctggtag
    13141 tcctggaacg aagtccggtg atcggcgaca ctggtgttga ggtagatata ccaacccttc
    13201 gcggggccac cttcatcggt caaccgctgc aggattgact cataaaagcc gtagcggcct
    13261 ctttgatccc ccagattttg gtctgggaca ccaccgacgc ctgggatggt cgtaatgata
    13321 cccggcaggc caccatcaat cgtcgacgca ctgtcatacc agccgcccaa tctccaggtc
    13381 ccgtacaggt gaccgcccgg cctccaggtc aactccaccg gcaccagcac ccccgaggcg
    13441 gggctagagg cagggatgcc cggcgccagg tagacgccca tgtctgacgt cgtcagataa
    13501 ttcggattgg cgtcgtagac gccggccgaa atctggactt ccttggtgat gttgaagtgg
    13561 gcgagaccgg cccactggct caccgggaag tt
//
LOCUS       DATNOI010000518        12311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_28417,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000518 DATNOI010000000
VERSION     DATNOI010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12311
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28417"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /gene="rpsD"
                     /locus_tag="VN968_20880"
     CDS             complement(<1..426)
                     /gene="rpsD"
                     /locus_tag="VN968_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXL31726.1"
                     /translation="MTKRSEAKYKIDRRMGQNIWGRPKSPVNRREYGPGQHGQRRKGK
                     LSDFGVQLRAKQKLKGYYANISERQFHGIYVEAGRMKGDTGENLIGLLERRLDTVVYR
                     AKFVATMFAARQFINHGHVKVNGRRVNIPSFKVKVGDLVE"
     gene            1277..1483
                     /locus_tag="VN968_20885"
     CDS             1277..1483
                     /locus_tag="VN968_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31727.1"
                     /translation="MEYRGKQYNVVQGIGPDSWRWTVHLDEKTIKSGQATSRAAAMNS
                     VVWLIDKALKPKKENPEAPSGEMF"
     gene            1612..2004
                     /locus_tag="VN968_20890"
     CDS             1612..2004
                     /locus_tag="VN968_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL31728.1"
                     /translation="MALEDQLFILVVEDNEEIQTIVEDALTEGGFEPAVAPSGEEAVT
                     LLRGHRGKYRALVADIVLRGRIDGWEVAQRAREIDPDFPVIYMTGVKADQWPSRGVPN
                     SVLMEKPFAPAQLVTAVSQLLNGGPPAS"
     gene            2069..2239
                     /locus_tag="VN968_20895"
     CDS             2069..2239
                     /locus_tag="VN968_20895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31729.1"
                     /translation="MTDQHQVHSIIATAICDALKKNPDHRMNTEEAKQVAKCIVETLT
                     DAGLQMVPLSNN"
     gene            complement(2297..3076)
                     /locus_tag="VN968_20900"
     CDS             complement(2297..3076)
                     /locus_tag="VN968_20900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXL31730.1"
                     /translation="MTPATPQPLNRLRKLWREGRPTFGAIATIPSIQTVQIMARSGFD
                     WIVVDLEHGPIDLGSAHAMITATSGTSCIPPARIAANEPWLAKAPMDIGALGINFPMI
                     CSREDAERAVRSVRYPPRGDRLWGPFHAPFRWGVSMPDYMATADDDMICMITIEHVEA
                     VNRIDEIMATPGIDMAVIGPGDLATSIDKRGRADDPEVLALMARAEAGILKSGVPIGG
                     VARTPEQANQMIERGYLGLALGFDWSLFQRGIAASFDGIKR"
     gene            complement(3162..3959)
                     /gene="murI"
                     /locus_tag="VN968_20905"
     CDS             complement(3162..3959)
                     /gene="murI"
                     /locus_tag="VN968_20905"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HXL31731.1"
                     /translation="MASPPTILVFDSGLGGLTVLREIVRTRPDAHYIYVADDAFFPYG
                     HHAEDRIIARVVPLIGELIAAHAPDLVVIACNTASTLVMSQLRAEYKVPFVGTVPAIK
                     PACASSKTRRVSVLGTKGTVKREYTKALIRDFAQGCEVTLVGSAQLASLAEAALSGVE
                     VNDLKISAELAPCFIGEGRNDPARTDTIVLACTHYPLLMDRFRRLAPWPVDWIDPAPA
                     IARRVTDLLRAAGSEADHAGAEMIFTSGRRHTLSRALMPFFGGRVPA"
     gene            complement(4039..5346)
                     /gene="purB"
                     /locus_tag="VN968_20910"
     CDS             complement(4039..5346)
                     /gene="purB"
                     /locus_tag="VN968_20910"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXL31732.1"
                     /translation="MISRYARPEMASIWEAQTRFKIWFEIEAHAADALAELGVIPKEA
                     ARAIWAKARDVTFDVARIDEIERETRHDVIAFLTHLAEIVGPEARFVHQGMTSSDVLD
                     TCLNVQLTRAADLLVEDIDKVLTALKKRAFEHKMTPTIGRSHGIHAEPVTFGLKLAYA
                     YAEFSRARARLLTARKEVATCAISGAVGTFAQIDPSVEAHVAKAMGLKPEPISTQVIP
                     RDRHAMYFATLGVIASSVERLATEIRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVL
                     SENLTGLSRMVRAYVTPAMENVVLWHERDISHSSAERMIAPDATVTLDFALNRLAGLI
                     DKLLVYPENMRKNLDRLGGLVHSQRVLIALTQKGCSREDAYKLVQRNAMPVWRGEGDF
                     QTLLKKDTEVKKYLSDAEISEQFDLGYHLKHVDTIFKRVFGES"
     gene            5732..6328
                     /locus_tag="VN968_20915"
     CDS             5732..6328
                     /locus_tag="VN968_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HXL31733.1"
                     /translation="MNETVVLTPERILEVTEDVLRRFGLAKATVVDVARALDVSHGSV
                     YRHFPSKASLREAVAKRWLDRANAPLAKIAEASGPAPARLERWLRTMFAIKHKKVSDD
                     PEMFATYLALAQEACKVVKAHKDRLVDQIAHILADGVKQGVFQVGDVNASARAIFDAT
                     SRFHHPAHAEEWSDPQLAARIDALLALLLKSLEAPRKR"
     gene            complement(6373..7749)
                     /locus_tag="VN968_20920"
     CDS             complement(6373..7749)
                     /locus_tag="VN968_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXL31734.1"
                     /translation="MALGEKSLNSRVPASRRTFVKGLGAAGATLSGVATWPRWSFAEE
                     AAAMYASAAIDWKQFSGQTITLAGAIHPWSNAIAALLPQFTKLTGISVVTDFQLETAF
                     LGALPIKLAGGSRTPDVFMFLTYGQGISAGWLEPLNAHYSDRLLTDLGWYDENDLLKT
                     AGAFSVWSNGERYAFPITSEAVTLFVNSDALAAKNLSVPQTFDELLATAKAVKTNEMS
                     GIAMRAQAGGNSSPAAMSYVFSYGGHMVKDNQAAFASPEAIAAVAIYGETLRQAGPVG
                     VGSYEWYHVLNDFVQGRTAMAIDSSVLAKDISDPANSRVAKQAAFAAFPHADGRSPVP
                     FMSHWQACINSRSQNKRATFLFLLWATSKPTSLQTAASGLATTRLSAWSSEGFKKAFG
                     PQAAAAALTNLQNADVDRAKAILFHPQSRPIQDAFMIGVNEVVSGAKSAKSAMTAAAE
                     KANAAIRG"
     gene            7971..9809
                     /locus_tag="VN968_20925"
     CDS             7971..9809
                     /locus_tag="VN968_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HXL31735.1"
                     /translation="MPFAASRPSSRWFAHPLILGVAVLLMLVAASGFLGVRYWQQRQA
                     ASRAVEHSRQVLDTLDRLRANIADLDTERRGYLLTLDPAYLKPYGISDESVRRESEAL
                     QALVADDPLQSLRAAHLALTVAAKLREIDEIVKTARTSKVDPALATIGTMGEIRSQID
                     QMIDNERFLLADWETRADALEQSKTRLIASAVAIVTVFAGGALALARLEARRRRKATE
                     ENVQLHSDLEEREKKIRHLVDANIIGIIIWNLEGRFLEANDAFLRMTGYDREDLASGR
                     VNRTDLTPAEWREADQRSLAEVRAIGSTQPYEKEYFRKDGSRVPVLIGGALFEWKRDE
                     GVAFVVDMTDRKRAEAEARESERRYRETLMELAHANRVTTMGQLTASIAHEVNQPIAA
                     TVINAEAALRWLGAQPPDLEEVREALGLIVEDGTRAGNVIGGIRALIKKVPPRQACLD
                     INEAIREVIALTRGEAAKTGVSVQTDLADGLPLIYGDRVQLQQVILNLIINAIEAMSG
                     VAETPRALQISTGQAEQGGVLVAVRDSGPGLDPASVERLFNAFYTTKSGGMGMGLAIC
                     RSIIEAHEGQLCASANEPRGAVFQFTLPLQRDEGVPAERAGPVPAA"
     gene            9945..10685
                     /locus_tag="VN968_20930"
     CDS             9945..10685
                     /locus_tag="VN968_20930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXL31736.1"
                     /translation="MRLAVFTDIHANRQAFSACLDFARARGAERIICLGDYVGYGADP
                     EWTVDVVMDLVERGAIAVRGNHDSAIGTMRVSMNAEAQAAIEWTRGRLSAAQRRFLAE
                     LPLTLQEDDRLYVHSEASNPQRWHYVQTTLDAARSIEATQAQITFCGHIHQPTLYSRS
                     SAAKMTSFVPRSGVPLQLLPGRRWLAVLGSVGQPRDGDPAASFAMFDTDSREITYCRV
                     PYDVETAARRIRQNGLPYWLADRLLIGR"
     gene            10688..11032
                     /locus_tag="VN968_20935"
                     /pseudo
     CDS             10688..11032
                     /locus_tag="VN968_20935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
     gene            complement(11107..11808)
                     /gene="rpe"
                     /locus_tag="VN968_20940"
     CDS             complement(11107..11808)
                     /gene="rpe"
                     /locus_tag="VN968_20940"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXL31737.1"
                     /translation="MSQQFASRPLVIAPSILASDFARLGEEVRAVDAAGADWIHLDVM
                     DGHFVPNISYGPDVIKAMRPHSKKIFDAHLMISPADPYLEAFAKAGCDHITVHAEAGP
                     HLHRSLQAIRALGKKAGVSLNPATPITAIEYVIDMIDLVLVMSVNPGFGGQAFIPSAV
                     GKILDLRAMTAGRPIDIEVDGGVAADVAGKLAAAGANAFVAGSAVFRGGTMEAYKANI
                     SAIRNAAALARGEAV"
     gene            11797..11988
                     /locus_tag="VN968_20945"
     CDS             11797..11988
                     /locus_tag="VN968_20945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31738.1"
                     /translation="MLRHVRFPEPFKSLQQARVTRASLAGNAGVIARGEAEISAVTAS
                     IAGVAGPFDVQKAAFLLGF"
     gene            12067..>12311
                     /locus_tag="VN968_20950"
     CDS             12067..>12311
                     /locus_tag="VN968_20950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HXL31739.1"
                     /translation="MLMALGVVSTALDVLQSLTSSKPSSPPSTGFGQGATKPFDFPGN
                     GPASGSSPPPWSGGNSQISPATMSALLAAQSQSSAGSA"
BASE COUNT         2341 a         3715 c         3963 g         2292 t
ORIGIN      
        1 ctcgaccaaa tcgccgacct tcaccttgaa gctcgggata ttgacgcggc ggccgttcac
       61 cttgacgtgg ccgtgattga tgaactggcg cgcggcaaac atggtggcca cgaatttggc
      121 gcgatacacc acggtgtcga gccggcgctc gagcaggccg atcaggtttt cgccggtatc
      181 gcccttcatc cgcccggcct cgacatagat gccatggaac tggcgttctg agatgttggc
      241 gtaatagccc ttgagcttct gcttggcgcg cagctgcacg ccgaagtcgg agagctttcc
      301 tttgcggcgc tggccgtgct ggccgggacc gtattccctg cggtttacgg gactcttcgg
      361 gcggccccag atattctggc ccatgcggcg atcgatcttg tatttcgcct cactgcgctt
      421 tgtcatcgcg tcctcttttt caagttcggt tttgaggaaa cgcgccctcc tgtgcgccgg
      481 gaaattcccg gagccgacag gcccgcccct taaagcttga agggatggac cacgggtcgc
      541 gaaacgcatc gcgggccgaa accggcccgc gagcaggcgg tttctaggga gaaacggccg
      601 tcctgtcaat gaaaacggcc ctggttcgag agggcgacag gactgtcatt ccgggctgga
      661 tacccaacac aaccgaccgc tgggccgacg aacgaagtcg gggcgtagca tcgatgccta
      721 ttccagcgca atcgccttga ttttgcttgg tatttgggcg ctatcccgta gcttttgaag
      781 tcatggggat gcatccgggc gcgggctgac atagcttttg gtcatgtcgc aagatgcccc
      841 gccaatgaat gacgaccaaa gtccgctgac gacactcatt tcgtgcgtcg actgtgcgaa
      901 gaccatgcga attgagaaga ttgaccccga cggcaagggc cgggacatga ttcaatatcg
      961 gtgcgattgt tgcggtcgga tcgaacggct gcgtcttatt cgccgaacct ggccctcggc
     1021 gcgacaaacg agaaggtgac acaaagcgga tttcagtgcg gccgttcgcg cgggcggtgg
     1081 cccgaacgcc ggtgcatccg gaaggggcca cgaggcaaaa cggccccacc ggggaccgcc
     1141 tcagaccggt aggtggagtg ccgagcggca ggaaaggccc ggcacgcaac cctgccgatt
     1201 tgttcctcta ccccaaaact attattgcgc gaacgatctg ttgctccgcc cgttgaatcg
     1261 tcgggcgagg agcgaaatgg aatatcgcgg caaacaatac aacgttgtcc aaggcatcgg
     1321 gcctgattcc tggcgatgga cggttcacct tgacgaaaag acaatcaaat cggggcaggc
     1381 cacctcgcgg gcggccgcca tgaatagcgt cgtgtggctg atcgacaagg cgttgaagcc
     1441 caaaaaggaa aacccggagg ctccctccgg cgaaatgttc taattccaga acacgagtgt
     1501 ccgacgtctg ctctgggata tccagggtgc ccaagtttat tttttcattt gtccggaacc
     1561 gaaccccgat tttggcgtag aggagttggc gccttgaagg cggagggggc aatggcattg
     1621 gaagatcaac tattcatttt ggtcgtcgaa gacaacgaag agatacagac cattgtcgaa
     1681 gacgcgctca ccgagggcgg ctttgaaccg gcggtcgctc cgtcaggcga agaggcggtg
     1741 accctgctgc ggggacacag gggcaaatat cgcgcactgg tggccgacat cgttctgcgc
     1801 ggcaggatag atggctggga agtcgcccag cgagcgagag agatcgaccc ggattttccc
     1861 gttatctaca tgaccggcgt caaagccgat caatggccct cgcggggcgt tccaaacagt
     1921 gtcctcatgg aaaagccgtt cgctccggcg cagcttgtca ccgccgtttc ccagcttctc
     1981 aatggcgggc cgccagcatc ctgaagccgg cgcggcctca cctttcgggt tctcatcgct
     2041 gtgctctcga caaacattgg gcgacacgat gactgatcag catcaggtgc attcgataat
     2101 tgccaccgca atctgtgatg ctctgaaaaa gaacccggat catcgaatga ataccgagga
     2161 agctaaacaa gttgcgaaat gcatcgttga gacgctaacc gacgccggcc tgcagatggt
     2221 gccgctcagc aacaactgag tgagcgcggt tggacggcgc gatcaattcc aaaaattttc
     2281 agcaccttgg cccggttcag cgcttgatgc cgtcaaaact cgcagcgatc ccgcgctgga
     2341 acagcgacca gtcgaagccg agcgcgagcc ccagataacc gcgctcgatc atctgattgg
     2401 cctgttccgg cgtgcgcgcc accccaccga tcgggacccc gcttttgagg atgccggcct
     2461 cggcgcgcgc catcagcgcc agcacctcgg ggtcgtcggc gcggccgcgc ttgtcgatcg
     2521 aggttgcgag gtcgccgggt ccgatcactg ccatgtcgat gccgggcgtc gccatgatct
     2581 cgtcgatgcg gttgaccgcc tcgacatgct cgatggtgat catgcagatc atgtcatcgt
     2641 cggcggtcgc catataatcg ggcatcgaga cgccccagcg aaacggcgcg tgaaacgggc
     2701 cccagagccg gtcgccgcgc ggcggatagc gcacgctgcg gacggccctt tcggcatcct
     2761 cgcggctgca gatcatcgga aaattgatgc cgagcgcgcc gatatccatc ggcgctttcg
     2821 ctagccaggg ctcgttggcc gcgatccgcg ccggcggtat gcaggatgtc cctgacgtcg
     2881 ctgttatcat tgcgtgcgcg gagccaaggt cgatcggacc gtgctcgaga tcgacgacga
     2941 tccagtcgaa tcccgagcgg gccatgatct gcacggtctg aatgctggga atggtcgcga
     3001 tcgctccgaa tgtcggacgt ccttcccgcc acagcttgcg gagacggttg agcggttgag
     3061 gcgtcgccgg ggtcaaatcc aaagctccgt ttcgatcgcg agaatgccga ttggttcgaa
     3121 agctaccagc gcggcgagat gtcgaaaaga tcaagctgca ttcacgccgg gacgcggccg
     3181 ccgaaaaacg gcatcaacgc ccggctcagc gtgtgccgcc gtccggaagt gaaaatcatc
     3241 tcggcgccgg catgatcggc ttcgctgccg gcggccctga gcagatcggt aacgcggcgg
     3301 gcaatcgcgg gcgccggatc gatccagtcc accggccatg gcgccagccg gcggaaccga
     3361 tccatcagca atggataatg ggtgcaggcg agcacgatgg tgtcggtgcg cgccggatcg
     3421 ttcctgccct ctccaataaa gcatggcgcg agctcggccg atatcttgag atcgttgacc
     3481 tcgacgccgc tcaaagccgc ctccgccagc gaagcgagct gcgccgagcc caccagcgtt
     3541 acttcgcagc cttgcgcgaa atcacggatc agcgccttgg tgtattcgcg cttgacggtg
     3601 cccttggtgc cgagcaccga cacgcgtctt gtctttgaac ttgcgcaagc cggcttgatc
     3661 gccggcaccg tcccgacaaa gggtaccttg tattcggcgc gaagctgcga catcaccagc
     3721 gtcgaggcgg tgttgcaggc gatcaccacc agatccgggg catgcgcggc aatcaattca
     3781 ccgatcaacg gcaccacgcg cgcgatgatc cggtcctcgg cgtgatggcc gtagggaaag
     3841 aacgcatcgt ccgcgacata aatgtaatgc gcgtcggggc gggtccggac gatttcacgc
     3901 aataccgtga gcccgccaag gccggaatcg aacaccagaa tcgtcggagg tgatgccacg
     3961 ccgcaatcct agccgctgcc gggttaccat tcgattgtca ttgcagcccc gtgaggcatg
     4021 cgcggcccgc ttcccgcgtc agctttcccc gaacacgcgc ttgaagatgg tatcgacgtg
     4081 cttgaggtgg tagccgaggt cgaactgctc ggatatctcg gcgtcgctga gatacttctt
     4141 cacctcagtg tcttttttca acagcgtctg gaagtcgcct tcgccgcgcc acaccggcat
     4201 ggcgttgcgc tgcacgagct tgtaggcgtc ctcacggcta cagccttttt gcgtcagcgc
     4261 gatcagcact cgctgcgaat gaacgaggcc gccgaggcgg tcgaggttct tccgcatgtt
     4321 ttcagggtaa accaacagct tgtcgatcag gccggcgagg cggttaaggg caaaatccag
     4381 cgtcaccgtc gcgtcgggcg cgatcatgcg ttcggcggaa gaatgcgaga tgtcgcgctc
     4441 gtgccagagc acgacattct ccatggccgg cgtcacatag gcgcgcacca tgcgcgacag
     4501 gccggtgaga ttttccgaca gcaccgggtt gcgcttgtgc ggcatcgccg acgaaccctt
     4561 ctggccctcg gagaagaatt cctccgcctc cagcacctcg gtgcgctgca agtgccggat
     4621 ctcggtggcg aggcgctcga ccgaggatgc gatcacgccg agggttgcaa aatacatcgc
     4681 gtggcggtcg cgcggtatca cctgggtcga gatcggctct ggcttaagtc ccatcgcttt
     4741 tgcgacatgg gcttcgacgc tcggatcgat ctgcgcgaag gtgccgaccg cgcccgagat
     4801 cgcgcaggtc gcgacttcct tgcgggcagt caggaggcgg gcgcgggcgc gtgaaaattc
     4861 cgcataggcg taggcgagct tcagcccgaa cgtcaccggc tcggcatgga tgccgtggga
     4921 gcggccgatg gtcggcgtca tcttgtgctc gaaagcgcgc ttcttcagcg ccgtcagcac
     4981 cttgtcgatg tcctcgacaa gcaggtccgc ggcgcgggtg agctgcacgt tgaggcaggt
     5041 gtcgagcacg tcggaggagg tcatgccctg gtgcacgaag cgcgcctccg ggccgacgat
     5101 ttccgccaga tgggtgagga aggcgataac gtcgtgcctg gtttcgcgtt cgatctcgtc
     5161 gatccgcgcg acatcgaagg tgacgtcgcg ggccttggcc cagatcgccc tcgcggcctc
     5221 tttgggaatg acgccaagtt ccgccagcgc atccgccgca tgcgcctcga tttcgaacca
     5281 gatcttgaag cgggtttggg cctcccagat cgaggccatt tccgggcggg cgtagcggga
     5341 aatcatcgac gactcttgta aaaaggggct cacgccgcgc tttagcagag cggcggaggg
     5401 gagacaaacc ggccgcaggg cagggccgac ggccacagtg tccggcaccg gccgctcgat
     5461 cacgaactat tgactgacag atattgaaat ctgttacccg gatgtcctat atccgatgtg
     5521 acgccgttgc gtgaggcgtc ccttggctcg ttggccatcc gaccgcgagc cccggacgtg
     5581 cagaggacta caccatgacg atcgaaattc tttatctgac ccatcaactc cagcgctgga
     5641 tcaacaggcg cgtggcccgg catcttgccg cgcaggcggc gaggctgagc cgctaaacag
     5701 taccgaacaa cgaaaggcag atggtgagcg catgaacgaa accgtggttc tgaccccgga
     5761 gcggattctc gaggtcaccg aggacgtgct gcgccgcttc gggctggcca aggccaccgt
     5821 cgtcgatgtc gcccgcgcgc tcgatgtcag ccacggcagc gtgtaccgcc attttccgag
     5881 caaggcttcg ctgcgcgaag cggtagcaaa gcgctggctc gatcgcgcca acgcgccgct
     5941 tgcgaagatt gctgaagcgt ccgggcccgc gcccgccagg ttggagcgct ggctgcgtac
     6001 catgtttgcg atcaagcaca agaaggtttc cgacgatccc gagatgttcg cgacctatct
     6061 ggcgctggcg caagaggcgt gcaaggtggt gaaggcgcat aaggatcgtc tggtcgacca
     6121 gatcgcgcac attctggctg atggcgtcaa gcagggcgtg ttccaggtcg gcgacgtcaa
     6181 cgcttcggcg cgcgcgattt ttgacgccac cagccgcttc catcatcccg cccatgccga
     6241 ggaatggagc gatccgcagc tcgcagcgcg gatcgacgcc ctgctggctc tgttgctcaa
     6301 gagtctggaa gccccgcgca agcgctaatg gtttaagtcc gacgctcgat aacagcgccg
     6361 ccaccggctc cgctatccgc ggatcgctgc attggccttc tcggcggcgg cggtcattgc
     6421 actcttcgcc gatttcgctc cggagaccac ttcattgaca ccgatcatga aggcgtcctg
     6481 gatgggtctc gattgcggat ggaaaaggat cgccttggcg cggtcgacat cggcattttg
     6541 caagttggta agcgccgcgg cggcggcttg tgggccgaac gccttcttga agccctcgct
     6601 cgaccaggcc gacagacgcg tcgtcgccaa cccggacgcg gccgtctgca acgatgtcgg
     6661 cttacttgtc gcccacagta ggaacaggaa cgtcgcccgt ttgttttgcg atctggaatt
     6721 gatgcacgcc tgccagtgcg acatgaaggg gacgggtgag cgaccgtcgg catggggaaa
     6781 ggcggcaaag gctgcctgct tggcgacgcg gctgttcgcc ggatccgaaa tgtccttggc
     6841 aaggacgctg ctgtcgatcg ccatggcggt cctgccctga acgaagtcgt tcaacacatg
     6901 gtaccattca tagctgccga cgccgacggg ccccgcttgg cgaagggtct ctccgtagat
     6961 cgcgacggct gcgatcgcct cggggctagc gaaggcggcc tggttgtcct tgaccatgtg
     7021 cccgccatac gaaaatacgt agctcatggc cgccggggat gagttgccgc cggcctgggc
     7081 ccgcatggcg atccccgaca tctcgttggt cttgaccgcc ttggcggtcg caagcagctc
     7141 gtcgaaagtc tgaggaaccg acaggttctt ggctgccaac gcatcgctgt taacgaacaa
     7201 ggtcaccgcc tcggaagtaa tcgggaaggc gtagcgttcg ccgttcgacc agaccgaaaa
     7261 ggctccggcc gtcttgagga ggtcgttctc gtcgtaccag ccgagatcgg ttagcaacct
     7321 gtcggaataa tgggcattca gtggctcgag ccatcccgcc gagatgccct ggccataagt
     7381 caggaacatg aagacgtcag gcgttctact gccgccggcg agcttgatcg gcagggcacc
     7441 caggaatgcg gtctccaact ggaaatcggt gacgacgcta atgccggtga gcttggtgaa
     7501 ctgcggcaag agcgcggcga tggcgttcga ccaaggatgg atcgcgcctg caagcgtgat
     7561 cgtctggccg gagaattgtt tccagtcaat cgcagcgctg gcatacatcg ccgcggcttc
     7621 ctctgcgaag ctccatcgcg gccacgtcgc cacgcccgag agtgttgctc cggcagcgcc
     7681 caatcccttg acgaaagtgc gccggctggc tggcactcga ctgttcagcg acttttcccc
     7741 cagcgccatg tcggtgctcc ttttgtggca atcaatcaca acacggcagg tcggacgctc
     7801 tcgcaagacg aatggataga ccccggaagc agcgaaacct gctcgcttga aattgtcagg
     7861 gctcctgtct cgcctaggcg gaatgatccg cgtgttctac tattctatca atactctggc
     7921 gtccgcacca gaagacgtgc gggtcgcgct cgcatggaag ggccaagtat atgccgttcg
     7981 ccgcgtcacg cccgtcctcg cggtggttcg ctcatccgct gatcttgggg gtcgcggtgc
     8041 ttctaatgct ggttgcggca agcgggttcc tgggcgtgcg gtattggcaa cagcggcagg
     8101 cggcaagccg cgcggtcgag catagccgcc aggtgctcga tacgctcgat cggctgcggg
     8161 cgaatattgc cgacctggac accgaaagac gcgggtatct attgaccctg gacccggcat
     8221 atctcaagcc ctacggcatc tctgacgaaa gcgtgcgacg ggagagcgag gcgcttcaag
     8281 ctctcgtggc ggacgatccg ttgcagagcc ttcgtgccgc acatctagcg ctgaccgttg
     8341 cggcaaagct gcgcgagatt gacgagatcg tcaagacggc gcgcacgtcc aaggtggatc
     8401 cggcgctggc gacgattggg accatgggcg aaatccgatc gcaaatcgac cagatgatag
     8461 ataacgagcg cttcctgctt gcggattggg agacgcgcgc cgacgcgctc gaacaaagca
     8521 agacgaggct gatcgcttcg gcggtcgcca ttgtcaccgt tttcgccggg ggcgccttgg
     8581 cgctcgcacg gctcgaagca cgacggcgac ggaaagcgac cgaggagaac gttcaactcc
     8641 acagcgatct tgaggagcgc gaaaagaaga tccggcacct ggtcgacgcc aacatcattg
     8701 ggatcattat ctggaatctc gaaggtcgtt ttcttgaggc caatgacgcg tttctccgca
     8761 tgacgggata cgaccgagag gatcttgcct cgggtcgcgt gaaccgaacg gacctgacgc
     8821 cagcggaatg gcgcgaggca gaccaacgct ccctagcaga ggtgagggcg atcggaagta
     8881 cccagccgta tgaaaaagag tacttccgca aagacggcag ccgcgtgccg gttctgatcg
     8941 gcggcgcgct tttcgaatgg aaacgagacg aaggcgttgc ctttgtcgtt gacatgaccg
     9001 accgcaagcg ggcggaagca gaggctcgcg aaagcgagcg gcgctatcgc gagaccctga
     9061 tggagctcgc gcacgccaat cgcgtcacca cgatggggca gctgacggcc tcgatcgccc
     9121 atgaggtcaa ccagccgatc gccgcgactg tcatcaatgc tgaggccgcc ttgcgctggc
     9181 tgggcgctca accgcccgat ctcgaggagg ttcgcgaggc cctcggcctt atcgtcgagg
     9241 acggcacgcg agccggcaat gtcatcggcg ggatccgggc cctcatcaag aaggtgccgc
     9301 cgcggcaggc ttgcctggac atcaacgagg caatccgcga ggtgattgcc ctgacccgtg
     9361 gcgaagcggc aaagaccggc gtttcggtgc aaacggatct cgcggacggc ttgccgctca
     9421 tttacggcga tcgggttcag ctgcaacaag tgatcctgaa cttgatcatc aatgctattg
     9481 aagccatgag cggcgtcgcc gagacacccc gcgcattgca gatcagcacc gggcaggccg
     9541 agcaaggcgg cgtgttggtc gcggtgcgag attcgggtcc gggactggat ccggcgagtg
     9601 ttgagcgcct cttcaatgca ttctacacga ccaagtccgg cggcatgggc atgggactgg
     9661 cgatctgtcg ttcgatcatc gaggcgcacg agggacagct gtgcgcctcc gcgaatgaac
     9721 cgcgcggcgc cgtctttcag ttcaccctgc ctctacaacg agacgagggc gttcccgccg
     9781 agcgcgccgg cccagtgccg gcggcatgag gttgcaggtc agggagagaa gcggcgtgcc
     9841 cccgatcccg gccttcagcg tggttgcgcg cctcgggccg cgtgttgttt gatgtccatc
     9901 aaatccggcc ccggcgtggg cgatatggta aggtggagga aagcgtgcgg ctggccgtct
     9961 tcacggatat ccatgccaac cggcaggcct ttagcgcgtg cctcgatttc gcgcgggcgc
    10021 gcggcgcgga acggatcatt tgcctcggcg attacgtcgg atatggcgcc gatccggaat
    10081 ggacggttga cgtggtgatg gatctcgtcg agcgcggggc gattgcggtg cgcggcaacc
    10141 atgacagcgc catcggcaca atgagggtat ccatgaatgc cgaagcccag gccgcgatcg
    10201 aatggacgcg cggcaggctc agtgcggccc agcggcgatt tctggcggag ctgccgctga
    10261 cattgcagga ggacgaccgc ctctacgttc attccgaagc cagcaatccc cagaggtggc
    10321 actacgtgca gacgacactg gatgccgcgc gcagcatcga ggcgacgcaa gcccagatca
    10381 cattctgcgg ccacattcac cagccgacgc tctattcgag gtcctcagcc gccaagatga
    10441 cgagcttcgt gccgagatca ggcgtgcccc tgcaattgct gccggggcgg cgttggctcg
    10501 ccgtgctcgg ctcggtgggg cagcctcgcg acggcgatcc ggcggcctcg tttgcgatgt
    10561 tcgataccga tagccgggaa atcacctatt gccgtgtgcc ctacgacgtc gagacggcgg
    10621 cgaggcggat ccgccaaaat ggcctgccat actggctcgc cgatcggttg ctgatcggga
    10681 ggtgagggtg gcgaaatttt cggttcagcc cggcgcggtc atcgatggtt tcacgatcgg
    10741 agagtgcatc catcacggcg gcatggcgac gctatggagc gtgacccatc ccggtttcgc
    10801 ggtgccgcta ttgatgaaga ttccgcgggt gtccgagggc gaggacccgg ctgccatcgt
    10861 cagcttcgag atggaacaga tgatcctgcc gcggttgtcg ggaccgcacg tgccgggctg
    10921 cttcggcacc ggggatttcg cgcggcaggc ctatgtcgtg atggagcgca tcccaggcaa
    10981 gacgctttac agccggctcg gcgagctgcc gttggcttac gaagaggcac gcgccacggt
    11041 ggtgcggccg ccgcggttgg cgtcagtgca ggagcaggag agtgcaaggc cggacgaggt
    11101 gagagcctag accgcctcgc cgcgcgccag cgccgccgca ttgcggatcg cagaaatatt
    11161 ggccttgtag gcctccatgg tgccgcccct gaacacggcg gaccctgcga cgaaggcatt
    11221 ggctccggcg gcggcaagtt ttcccgcgac atccgcagcc acgccgccgt cgacctcgat
    11281 gtcgatcggc cgccccgccg tcattgcgcg caggtccagg atcttgccaa ccgcggacgg
    11341 aataaaagcc tggccgccga aaccgggatt gaccgacatc accagcacca gatcgatcat
    11401 gtcgatgaca tattcgatcg cggtaatcgg cgtggccggg ttgagcgaga cgccggcctt
    11461 cttgccgagc gcgcggatcg cctgcagcga gcgatgcaga tgcggacctg cttcggcatg
    11521 gacggtaatg tgatcgcagc cggcttttgc aaatgcctcg agatacggat cggccggcga
    11581 gatcatcagg tgggcgtcga aaatcttttt gctgtgcggg cgcatcgcct tgatgacgtc
    11641 ggggccgtag gaaatgttgg ggacgaaatg cccgtccatc acgtcgagat ggatccagtc
    11701 ggcgcctgca gcatcgacgg cgcggacctc ctcgcccaac ctggcgaaat cggaagccag
    11761 gatcgacggc gcgatgacca gggggcgaga cgcaaattgc tgagacatgt gcgctttccg
    11821 gaacctttta agagcctgca gcaggctcgc gtaacacgcg ccagccttgc gggcaatgcg
    11881 ggcgtgatcg cgcgtggaga ggcggaaatt tctgccgtaa cggcatcaat tgccggggtc
    11941 gccggaccat tcgacgtgca aaaagccgcg tttctattgg gtttttgagc atggcacggg
    12001 gtttgctgat gtttcggccg ggaacgtaaa aggccgcgcc ctgcggcagc tgtcgggggt
    12061 tttgccatgc tgatggctct tggcgtcgta tcgaccgcgc tggatgtgct gcagtcgctg
    12121 acgtcgtcga aaccgtcgtc tccgccatcg accggttttg ggcaaggtgc cacgaagccc
    12181 ttcgatttcc ccgggaacgg accggcttca ggaagttcgc cgccgccctg gtccggcgga
    12241 aattcgcaga tatcgccggc taccatgagc gcgctgctcg cggcgcaaag ccagtcctcg
    12301 gcgggttcag c
//
LOCUS       DATNOI010000519        10836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1454313,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000519 DATNOI010000000
VERSION     DATNOI010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10836
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454313"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VN968_20955"
     CDS             <1..283
                     /locus_tag="VN968_20955"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXL31740.1"
                     /translation="NLDRSEGDVPENVFLGVAKGEIEMQIGQITEIVISPGVDLAGPL
                     PSEIQNTTLMAAGIVTTSKAPDAAKALISFISSPSAAAVLKASGFQPIN"
     gene            435..935
                     /locus_tag="VN968_20960"
     CDS             435..935
                     /locus_tag="VN968_20960"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HXL31741.1"
                     /translation="MSSVIDDYLVDPEVSLLDKMRMQAQVLVPVLRALRAELGKDRAD
                     ALVKQALRDWSKQLFAAIGDGIEGSPRRKWTTIQRVFGEVSGKEVEASILRHDKEALD
                     IDVTRCRFAEFFRALGEPELGALLICEADFDIVSVGEGEVSLERAQTIMRGAPSCTFR
                     YKFAPR"
     gene            complement(958..2226)
                     /locus_tag="VN968_20965"
     CDS             complement(958..2226)
                     /locus_tag="VN968_20965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HXL31742.1"
                     /translation="MTDEPPVTVIAASLTGAPRRGAVAFIFVTILLDMFALGLILPIL
                     PKLVESFVDNDTANAARIFGLFGTAWAVMQFLFSPILGGLSDRFGRRPVVLLSNFGLA
                     LDYVLMALAPSLTWLFVGRVISGITSASVSTAFAYIADLTPPDQRAAVFGKIGVAFGA
                     GFILGPAVGGLLGGMDPRLPFWVAAGLSFANTLYGLLILPESLPPDRRSPFHWRSANP
                     LGALHLLRSDRVLTGLSVANFLAQLAHVVLPSTFVLYATYRYGWDTTTVGLTLAIVGI
                     CSMAVQGAAIGPIVKRFGERRALLLGLGCGALGFLMYGAAPTGLLFWLGIPVMALWGV
                     AGAALQALTTQRVAPDQQGQLQGATSSVQSLSQLVGPFLFTLTFAYFIGAQAPLKLPG
                     APFLLASALLLLALVIAARTLAVAPAGRAP"
     gene            complement(2345..3322)
                     /locus_tag="VN968_20970"
     CDS             complement(2345..3322)
                     /locus_tag="VN968_20970"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXL31743.1"
                     /translation="MTDPECSTTVRFSTADLPENLRVATWREHYSHTVLRADIDPGDD
                     ASFDATVVSRTLPGLQLVSGRFTAARIVRTREMIADGNDDLSLLVNQTGSVTVTARGR
                     EVTLHENDAVMISSSEAVVFDRRSFGESIAIRIPYSILSSAVVDVDDAIMRHICRETP
                     ALRLLTSYSNTLLGDDHEMGSPVLRHQVATHMHDLTVLALGATREAADIAGNRGMGAA
                     RLKAAKTYIAENLQHRDISIGTVAAHLGMTPRHLQRLFENDGTTFSAFLLGRRLARAY
                     RMLCEPRSGEKQVSAVAYDVGFGDLSYFNRSFRRLYGATPMDVRAGVLK"
     gene            complement(3338..3520)
                     /locus_tag="VN968_20975"
     CDS             complement(3338..3520)
                     /locus_tag="VN968_20975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31744.1"
                     /translation="MASGHLSRQAETVIQLILIAALQRKTGLHRSGEAANFTAIYQFG
                     IGLRHYGMLADIADWR"
     gene            complement(3728..4864)
                     /locus_tag="VN968_20980"
     CDS             complement(3728..4864)
                     /locus_tag="VN968_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL31745.1"
                     /translation="MDQAEQLSSLIGEIYDAALDPSLWSGVLGKAGRFVGGPVAAIFA
                     KSPTALTGTVYYHSGDRDPSYRQLYFDKYIKFDPTTTAQYFSDVGQPMAVADIMPYQE
                     FLETRFYKEWCHPQGMVDAVTAVLDKSVTSAALFAVFRYQSEGVVDDETRRRMRLVVP
                     HIRRAVLIGRLIDLRAADAASLADTLDGLSAAMCAVDADGRIVHANAACHVLLDAGEF
                     VSVVRGRIVASDTKIDQTLRELFAAAGSGDAAIGTRGIALPLMVRDGSRYVAHVLPLT
                     SGARRLTGIAYSATAALFISKVTTEAPSAPEAIARAYHLTPTELRVLLAIVGVGGVPE
                     SAVALGIAESTVKTHLGRLFVKTGARRQADLVKIVAGFSSPLIG"
     gene            complement(5284..6417)
                     /locus_tag="VN968_20985"
     CDS             complement(5284..6417)
                     /locus_tag="VN968_20985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HXL31746.1"
                     /translation="MDQAEQLSSLIGEIYDAALGPSLWSDIVGKAGRFVGGSAASIYS
                     KSPTAMTVTVHYQSGIDPYYLQIYLDKYVKLDPTMIAHYFADIEQPIATADIIPHHEL
                     METRFYKEWVQPQGIAGNLSAVLDKSATSVALFAVFRYGQEGVVDDEMRQRMSLIVPH
                     IRRAVLIGGLIDLRSADAANLADTLDGLSAAMCAVDADGRIVHANAACRLLLDAGDFL
                     STAGGRIVASDAKIDQAFRELFAAAGRGDAAIGTRGIALPLKGRGGSHYVAHVLPLTS
                     GARRFAGIAYSATAALFICKTATETRSPPEIIARAYHLTATELRVLLAIVDVGGVPES
                     AVALGIAESTVKTHLGNLFVKTGARRQADLVKIVAGFASPLVG"
     gene            complement(6819..7955)
                     /locus_tag="VN968_20990"
     CDS             complement(6819..7955)
                     /locus_tag="VN968_20990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL31747.1"
                     /translation="MDQAEHLSELIGEIYDAALDPALWCDVVGKAGRFVGGSAAAIYS
                     KSPTALTGNLYYESGTDPHYGELYFNKYIKFDPSVTSHYFADVEQPIATADYMPYQEF
                     LETRFYKEWVRPQGWVDSLNAVLDKSVTSAALFVVFRHQRDGVVDDEARRRMRLIVPH
                     IRRAVLIGRLIDLRATDAANLADTLDGLSAAMCAVDALGRIVHANAACHILLDARDFL
                     SVIGGRLVASDAEIDRTLRELFAAAGSGDAAVGTQGIALPLRAKDGSHYAAHVLPLTS
                     GARRLAGMARSATAALFICKVAAEIRSPREVIARAYHLTPGELRVLLAIVDVGGVPES
                     AAALGVAESTVKTHLGNLFVKTGARRQADLVKIVAGFASPLIAN"
     gene            complement(8356..9741)
                     /gene="der"
                     /locus_tag="VN968_20995"
     CDS             complement(8356..9741)
                     /gene="der"
                     /locus_tag="VN968_20995"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HXL31748.1"
                     /translation="MSFTIAIIGRPNVGKSTLFNRLVGQKLALVDDEPGVTRDRREGE
                     GRLGDLEFTIIDTAGLDEGAKGSLTARMQEQTEAAIGLADALMFVVDARAGLTPNDRA
                     FADFARRANKPVVLVANKSEGKQGDAGAMEAYELGLGEPIQISAEHGEGLSDLYDALR
                     ALMPEPVEEPEAFDDDDVIASDEDIAKRPIRVAIVGRPNAGKSTLINHLLGEERLLTS
                     AEAGTTRDSISVELNWQGRDFRVFDTAGLRRRSRIEEKLEKLSVADALRAVRFAEVVV
                     LMMDAQNKFEEQDLRIADLIEREGRALVIAVNKWDLMGRQSSLIAGLRTDADHLLPQV
                     KGMPIVAVSGLMGEGIDRLMSAIEQAYAVWNRRVPTASLNRWFEQAVDNNPPPAVSGR
                     RLKLNYITQAKARPPSFILFCSRADAVPQSYLRYLVNSLREFFELPGTPVRISLREKA
                     NPFAHKRKRPS"
     gene            complement(9903..10463)
                     /locus_tag="VN968_21000"
     CDS             complement(9903..10463)
                     /locus_tag="VN968_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31749.1"
                     /translation="MRRSQRLIASAVVIALSGVLAGCSSGFGNWDPTDLLDFLDTKKK
                     LPGERKPVFPDGVPGLEQGVPKELYKGSAQQQLDQQNAQAAAAAAAPAPEEPKSKSAA
                     KSARKGKQPAAASAAAAPDPTAAGPDPDVAPAEDGSTAAAPPAPKPKKIVRRRTTAPP
                     PDSAQPAQPPQQSAFPAPMPSGTFTR"
     gene            complement(10494..>10836)
                     /locus_tag="VN968_21005"
     CDS             complement(10494..>10836)
                     /locus_tag="VN968_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31750.1"
                     /translation="RDPQAAVKLYDEMSNDRSIGAPEQDLAKIRAAQLLLETSGYPNM
                     LSRLEPATAPAATFRHTARELLALSAWRANDTAAARQWLDLIANDSETPPSLRSRAEA
                     LQALLPPVAKS"
BASE COUNT         2121 a         3432 c         3387 g         1896 t
ORIGIN      
        1 caatcttgac aggtcagagg gcgatgttcc cgagaacgtc ttcctggggg ttgcgaaggg
       61 cgaaattgaa atgcaaatcg gccagataac cgagattgtg atctcgcccg gcgtcgatct
      121 tgctggtccg ctccctagcg agattcagaa taccactttg atggcagctg gaatcgtcac
      181 gactagtaaa gcgccagacg ctgccaaggc actcattagc tttatatcat ctccctccgc
      241 tgccgccgtc ttgaaggcaa gcggattcca gccgatcaat tagaattaag cccgattggc
      301 tgccgcgccg acagagtcgc cgcgtcgaac ctgccgcgcg tctccatcgc cggcaccgcg
      361 tcgccgtgtt tcagcgcggt cgcttgtgct ataatagagc cgccagactt ccgacgttgg
      421 tttgtgagga cagaatgagc agcgtcatcg acgactatct cgtggatccc gaggtatccc
      481 tcctggacaa gatgcgcatg caggcccagg tcctggtgcc ggtgcttcgt gcgttgcgcg
      541 ccgaactcgg caaggacagg gccgatgccc tcgtgaagca ggccttgcgc gactggtcca
      601 agcagttgtt cgccgccatt ggcgacggca tcgagggcag cccgcggcgc aaatggacga
      661 ccatccagcg cgtctttggc gaggtctcgg gcaaggaggt cgaggccagc attctgcgcc
      721 atgacaaaga ggcgctggac atcgacgtga cgcgctgccg cttcgccgag ttcttccgcg
      781 cactgggcga gcccgagctc ggtgcgctgc tgatttgcga ggcggatttc gacatcgtgt
      841 cggtcggcga aggcgaagtc agcctcgaac gcgcgcagac aatcatgcgg ggcgcgccga
      901 gctgcacctt ccgctacaag ttcgcaccgc ggtaaatttt ctctgcgagg ccgccgctta
      961 gggagcacgc cccgcaggcg cgacggccag cgtgcgcgcc gcgatcacca gcgccagcag
     1021 caatagcgcc gacgccagca ggaacggcgc gcccggcaat ttcagcggcg cctgggcacc
     1081 gatgaaatag gcgaatgtca gggtgaacag aaacggtccg accagctggg acaagctctg
     1141 cacgcttgac gtggcaccct gcaattggcc ctgctggtcc ggcgcgaccc gctgcgtcgt
     1201 cagcgcctgc aacgcagccc ccgccacgcc ccacaatgcc atcacgggaa tgccgagcca
     1261 gaacaacaga ccggtcggcg ccgcgccgta cattagaaag cccagggcgc cacagccaag
     1321 gcccaatagc agcgcccggc gctcgccgaa acgcttgacg atcggcccga tcgcagcccc
     1381 ctgcacggcc atcgagcaga tgccgacaat ggccagcgtc agaccgaccg tggtcgtatc
     1441 ccagccatat cgataggtcg catagagcac gaaggtcgag ggcagaacca catgcgccag
     1501 ttgggccaaa aaattcgcca ccgacaaccc ggtgagcacc cggtcggagc gcaacagatg
     1561 cagcgcaccc agcggattcg cgcttctcca gtggaacggc gaacggcgat ccggcggcaa
     1621 cgactccggc aggatcagca ggccgtaaag cgtgttggca aagctcaagc cggccgcgac
     1681 ccagaacggc aaccgaggat ccatgccgcc gagcagcccg ccgaccgccg gtccaagaat
     1741 gaaaccggcg ccgaacgcca cgccgatctt gccgaacacc gccgcgcgct ggtcgggggg
     1801 cgtcagatcc gcgatgtaag cgaacgccgt cgagacgctc gccgaggtga tccccgagat
     1861 caccctgccg acgaaaagcc aggtcagcga cggcgccagc gccatcagca cgtaatcgag
     1921 cgcgagcccg aaattcgaca gcagcaccac ggggcggcgg ccgaaccggt ccgacaggcc
     1981 gccgagtatc ggcgagaaca gaaattgcat caccgcccag gcggtgccga acaggccgaa
     2041 gattcgcgcc gcattggcgg tgtcgttgtc gacaaaactt tccaccagct tgggcaggat
     2101 cggcaggatc aggccgagcg cgaacatgtc caacaggatg gtgacgaaga taaaggccac
     2161 cgcgccgcgg cggggcgcgc cagtcaggga cgccgctatg acagtcacgg gcggttcgtc
     2221 agtcatgatt tgcgcttgtg ttgatcggcg gatgggctgg atagcgggcg cagactaatc
     2281 catgcaccag tgcagtagca atgcagggca gccgcgccgg acgccatccg gtgacggcca
     2341 ggtgctattt caaaacgcct gctctgacat ccatgggtgt ggcgccgtag agccgtcgaa
     2401 agctgcggtt gaaataggaa agatcgccaa agccaacgtc gtaggcgacc gcgctgacct
     2461 gcttttcccc ggaccggggc tcgcagagca tgcggtaggc gcgggcgagg cgccgcccga
     2521 gcaagaaggc agaaaacgtg gtgccatcgt tctcgaacag tctctgcaga tggcgcggcg
     2581 tcatgccgag atgcgcggcg acggtgccga tcgaaatatc ccgatgctgg aggttctcgg
     2641 caatatacgt ctttgccgct ttcaatcgcg ccgcccccat cccccgattt cctgcaatgt
     2701 ctgcggcttc gcgggtagct ccgagcgcga gcaccgtcag atcgtgcatg tgggtcgcaa
     2761 cctgatgtcg aagcaccggc gaccccatct cgtgatcatc acccagcaag gtgttcgagt
     2821 agctggtcag cagtctgagc gcgggcgttt cccggcagat gtgacgcatg attgcgtcat
     2881 cgacatcaac gaccgccgac gacaatatcg agtatggaat tcgaatggcg atagattcgc
     2941 cgaaggaacg gcggtcgaac acgacggcct cgctgctgct gatcatcacc gcgtcattct
     3001 cgtggagcgt gacttcacgg ccgcgcgcag taactgtgac actaccggtc tgattcacca
     3061 gcagcgaaag atcgtcgttg ccgtccgcta tcatctcgcg tgttcgcacg attcgggcgg
     3121 cggtaaaccg gcccgacacc agttgcagtc ccggcagggt acgtgaaacc accgttgcgt
     3181 cgaacgaggc gtcgtcaccg ggatcgatgt cagcccggag cacggtgtgg ctgtaatgct
     3241 cgcgccaggt ggcgacccgc aagttttccg gaagatcggc cgtcgagaat cgaactgtcg
     3301 tcgagcactc tggatcggtc acgtggctca tttcccgtca gcgccagtca gcaatatcgg
     3361 ccaacatgcc gtaatgccgc aggccgatgc cgaattggta gatcgcggta aaattcgccg
     3421 cttcccccga tcggtggaga ccggtttttc gctgcagcgc agcgatcaaa ataagttgaa
     3481 ttacagtttc agcctgacgg ctgaggtgac ctgaagccac tttccggctt ccaatacaat
     3541 gacttaagtg tccatcctcg cggcgcgcat gaacgtcgcg gtagttcgcg acgtcaggcc
     3601 cacgacatca gaaatggcat cagaaacgac atctgaaatg acatcggatt ggacttcgcc
     3661 gctgacggct cagccggatg atgcggatta gccacacgcc ttgcagggat gacggggacg
     3721 atttgagcta gccgatcagc ggactggaaa atcccgcgac gattttcaca aggtcagcct
     3781 ggcgacgggc gccggtcttc acaaacagcc tgccgaggtg cgtcttgacc gtgctttcgg
     3841 caatgccgag ggccaccgcg ctttcgggga ccccgccgac cccgacgatg gcgagcagca
     3901 cccgcaactc ggtcggggtc agatggtagg cgcgggcgat ggcttccggc gcagaggggg
     3961 cttccgttgt gaccttgcta atgaacaggg ccgcggtcgc agagtaagcg ataccggtaa
     4021 ggcggcgggc gccggatgtc agcgggagga catgcgcgac atagcgcgag ccgtcccgca
     4081 ccatcagcgg caaggcaata ccccgagttc cgattgcggc gtcgccgctg ccggcggctg
     4141 caaacaactc ccgcaaggtc tgatcaatct tggtatcgct cgcgacgatc cttcccctga
     4201 ccacggaaac gaactcgccg gcgtcgagaa gcacgtggca ggcggcattg gcgtggacga
     4261 tgcgtccatc cgcatcgaca gcgcacatcg ccgcgctgag accatcgagg gtgtcggcga
     4321 ggctcgcggc atccgcggcc ctcagatcga tgagtctgcc gatcagcacc gcccggcgaa
     4381 tgtgcggaac gacgaggcgc atgcgccggc gcgtttcatc gtcgacaaca ccctcgcttt
     4441 ggtagcgaaa caccgcgaac aatgccgcgc tggtcaccga tttatcgagc accgccgtca
     4501 cggcgtccac catgccttgt gggtggcacc actccttgta aaaccgcgtt tccaggaact
     4561 cctggtaagg catgatatcc gcaacggcca tcggttgccc gacatcagag aagtattggg
     4621 cggtcgtggt ggggtcgaat tttatatatt tgtcgaaata gagctgtcgg tatgacgggt
     4681 ccctgtcgcc ggaatgataa tagacagtgc cggtcaaggc ggttgggctc ttggcaaaga
     4741 ttgccgccac cgggccgccg acgaaacggc cggccttgcc gagcacgccg ctccacagcg
     4801 agggatccag cgcggcatcg tagatctcac cgatgagtga cgaaagctgc tctgcctgat
     4861 ccatggtcat ccccgaagac gggcacttga aggtcgatcg tcgacatggt tgccgccgaa
     4921 ttacttgaac ccctcgagcc ttttccgttt cgatagaaat cgaaacggag cgctggattc
     4981 cctacttgac gcgttttctt gacgcgaccg gtcccacttc gcttgaaaac gctttggtat
     5041 attggcaagc cgcgagcccg gattccgtga ccgcgagcac agaactcccg ctcacgatgg
     5101 ccgcctatgt ttgtgggtta cttctcgcgc aacgcgatcc gcaccgtgtg cccggcaact
     5161 cacgcaagca gttgacgaga caacgaaggt cggattgagg cacggggtcc gcgaacctgc
     5221 gtcgctcgca tcgaaatgac gcctcgccag aaggccgccg gcggggcctg tcgatgaaat
     5281 ccgtcagccg accagcgggc tggcaaatcc cgcaacgatc ttgacaagat cggcctggcg
     5341 acgggcgccg gttttcacga acaaattgcc gaggtgcgtc ttgaccgtac tttcggcaat
     5401 gccgagggcc accgcgcttt cgggaacccc gccgacgtcg acgatggcga gcagcacgcg
     5461 caactcggtc gcggtcagat ggtaggcgcg ggcgatgatt tccggaggag accgcgtttc
     5521 cgttgccgtc ttgcaaatga acaaggccgc ggttgcggag taagcgatcc cggcaaagcg
     5581 gcgggcgccc gatgtcagcg gcagcacatg cgcgacataa tgcgagccgc cccgtccctt
     5641 cagcggcaag gcaatgcccc gggttccgat ggccgcgtcg ccgcggccgg ccgcagcaaa
     5701 cagctcacga aaagcctgat cgatcttcgc atcgcttgcg acgatccgtc cccccgccgt
     5761 ggacaggaaa tcgccggcgt cgagaaggag gcggcaggcg gcattggcgt ggacaatgcg
     5821 tccgtccgca tcgacagcgc acatcgccgc gctcagacca tcgagggtgt cggcgaggtt
     5881 ggcagcatcc gcggacctga gatcgataag tccgccgatc agcaccgccc ggcgaatgtg
     5941 cggaacaatg aggctcatgc gctggcgcat ttcatcgtcg acaacgccct cctgtccgta
     6001 gcgaaacacc gcaaacagag ccacgctggt cgccgatttg tcgagcaccg ccgatagatt
     6061 acccgctatg ccttgcggtt ggacccattc cttgtaaaac cgcgtctcca tgagctcatg
     6121 gtgaggtatg atatccgccg tggcgatcgg ttgctcgata tcagcgaagt agtgggcgat
     6181 catggtaggg tcgagtttca catatttgtc gagatagatt tgcagataat acgggtcaat
     6241 gccggattga taatggactg tgacggtcat ggcggtgggg ctcttggaat agattgatgc
     6301 cgccgagccg ccgacgaaac gcccggcctt gccgacaatg tcgctccaca gcgacggacc
     6361 cagcgcggca tcgtagattt cgccgatgag tgaagaaagc tgctctgcct gatccatggc
     6421 catccccgat gacggacact tgtaacgtcg atcgtcgata tggttgccgc cgagttacct
     6481 tgaacccccg gtgtattggc aagccgcgag ccccggattc cgtgaccgca agcacacaga
     6541 actcccgctc atgatcgccg cttgcgtttg tcgtcgaacg gattggcctt ctcgcgcaac
     6601 gcggtccgcg ccgtgtgccc gccaactcac gcaagcaatt gacgaggcaa cgaaggtcgg
     6661 attgaggcat gatgtccgtg aacctgcctc gctcacaccg gatcgaaatt gaggttcgat
     6721 ccaggcgcag cgacgacgcg gaggctggac cgaggcgacc gcgccctgaa tcgaaacgac
     6781 tcttggccag tggtcggcgg ccgggtctat cgatgacatc agttagcgat caacggactg
     6841 gcaaatcccg cgacgatttt cacaagatcg gcctggcgac gggcgccggt tttcacaaac
     6901 aaatttccga ggtgcgtctt gaccgtgctt tcggcaacgc cgagggccgc cgcgctttcg
     6961 ggaaccccgc cgacatcgac gatggcgagc agcacgcgca gctcgcccgg ggtcagatgg
     7021 taggcgcggg cgatgacttc ccgaggagat cggatttccg ctgcgacctt gcagatgaac
     7081 aaggccgcgg tcgcggagcg cgccatcccg gccaggcggc gggcgccaga tgtcagcggc
     7141 agcacatgcg cggcataatg cgagccgtcc tttgccctca gcggcaaggc aataccctgg
     7201 gttccgaccg ccgcatcgcc gctgccggcc gctgcaaaca gctcccgcaa ggtccgatca
     7261 atctcggcat cgcttgcgac gagccgcccc ccgatcaccg acaggaaatc gcgggcgtcg
     7321 agaaggatgt gacaggcggc attggcgtgg acgatgcgtc caagcgcatc gacagcgcac
     7381 atcgccgcgc tgaggccatc gagggtgtca gcgaggttcg cggcatccgt agccctgaga
     7441 tcgataagtc tgccgatcag caccgcccgg cgaatgtgcg gaacgatgag gcgcatgcgc
     7501 cgccgcgctt catcgtcgac aacaccgtcg cgttggtggc gaaacaccac aaacagcgcc
     7561 gcgctggtca ctgatttatc gagcaccgcg ttcaagctgt ccacccagcc ttgcggtcgc
     7621 acccactcct tgtaaaaccg cgtttccagg aactcctggt aaggcatgta atccgccgtt
     7681 gcgatcggtt gctcgacatc cgcgaagtaa tggctggtca cggaaggatc gaatttgata
     7741 tatttgttga aatagagttc gccataatgc gggtcagtgc cggactcata atagagattg
     7801 ccggtcaggg cggtcgggct cttggaatag attgccgccg ccgaaccgcc gacgaaccgc
     7861 ccggccttgc cgacaacgtc acaccaaagc gccggatcca gcgcggcatc gtagatctcg
     7921 ccgatgagtt cagaaaggtg ctctgcctga tccatggtcc tccccgaaaa cggacacccc
     7981 gcacaggtga ccgtcgacac aaccgtaacc caattgcctt caacccaatt gccttcaacc
     8041 gaattgtctt caactgaatt gccttggctg ctggcttgat acgtcaaacg tagggcgccg
     8101 gttcggcaag cgcggcacga agggcctcag acctgcccga cgccggcgtt cgccaacaag
     8161 ccatccagcg cggaagcttc cagcgggatt ctacacgcag cattggcgtc ctgcttggac
     8221 gtacgcgaca gcggatttgc gccaggcgac atcgcggcct taccaggcct cggctgagga
     8281 aagcgatacc ggcgatcaac gatggcgccg ccgccaaacg ccaaatcagt agtggggaga
     8341 ccgtctacgg attgttcacg atggccgctt gcgcttgtgg gcgaacggat tggccttctc
     8401 gcgcaggctg atccgcaccg gcgtgcccgg aagttcgaaa aactcacgca aggagttgac
     8461 gagatagcgc aggtaggatt gcggcacggc gtccgcgcgc gaacagaaca ggatgaaact
     8521 cggcggccgc gccttggcct gggtgatgta attcagcttc agccgccggc ccgacaccgc
     8581 cggcggcggg ttgttgtcga cggcctgctc gaaccagcga ttaagcgaag ccgtcggcac
     8641 ccgccggttc cacaccgcgt aggcctgttc gatcgcgctc atcagccggt cgatgccctc
     8701 acccatcaaa ccggacaccg cgacgatcgg cattcccttc acctgcggca gcaggtgatc
     8761 ggcgtcggtg cgcaagcctg cgatcaggct cgactgccgt cccatcaaat cccacttgtt
     8821 gacggcgatc accagcgccc ggccctcgcg ctcgatcagg tcggcgatgc gcaggtcctg
     8881 ttcctcgaac ttgttctgcg cgtccatcat cagcaccacg acttcggcaa aacgcaccgc
     8941 gcgcagcgcg tccgccaccg agagcttttc cagcttctcc tcgatccgcg agcgccgccg
     9001 cagccccgcg gtgtcgaaca cgcgaaaatc ccggccctgc cagttgagct cgacggaaat
     9061 cgaatcgcgg gtggtgccag cctcggcgct ggtcagaagc cgctcctcgc cgagcagatg
     9121 attgatcagc gtggacttgc cggcgttggg gcggccgacg atggccaccc ggatcggccg
     9181 cttcgcgata tcctcatcgg aagcgatgac gtcgtcgtcg tcgaacgctt cgggttcttc
     9241 gacgggctcc ggcatcagcg cgcgcagcgc gtcgtagagg tcgctgaggc cctcgccatg
     9301 ttcggctgaa atctggatcg gttcgccaag acccagctcg taggcttcca tggcgccggc
     9361 gtcgccctgc ttgccctcgc tcttgttggc gaccagcacc accggcttgt tggcacggcg
     9421 cgcgaaatcg gcaaaggcgc gatcgttcgg cgtgaggccc gcgcgcgcgt cgaccacgaa
     9481 catcagcgcg tcggcaagcc cgatcgccgc ctcggtctgt tcctgcatcc gcgcggtcag
     9541 cgagcccttg gcgccctcgt cgaggcccgc ggtgtcgatg atggtgaatt cgagatcgcc
     9601 gagacgcccc tcgccttcgc ggcggtcgcg ggtgacgccg ggttcgtcat cgaccagcgc
     9661 cagcttctgt ccaaccaacc ggttgaacag cgtggatttt ccgacattgg gccggccgat
     9721 aatggcaatc gtgaaggaca taagcgatcc cagcgctctt tatgaagcgc atgtcaatga
     9781 gatgaaaagc caagagcctc ggttcagctc tgacgcgaga gaccggaaca gaggctctaa
     9841 tgttatttcg acgcgtctgc ttcgcgcgaa ccggtacccg cttcgctcga aaacgctttg
     9901 gatcagcgcg tgaaagtacc gctcggcatc ggtgccggga aggcggactg ctgcggcggt
     9961 tgcgcgggct gcgccgaatc cggcggcggc gcggtggtgc gccgccgaac gattttcttg
    10021 ggcttgggag ccggcggcgc agcggcggtg ctgccgtctt ccgcgggcgc cacatcggga
    10081 tccggcccgg cggcagtcgg gtccggcgcg gccgccgcac tggcggcggc cggctgcttg
    10141 cccttgcgtg cggatttggc agcgctcttc gatttcggct cctctggagc cggcgcggct
    10201 gcggcggccg cggcctgggc gttctgctgg tccagctgct gctgcgccga gcccttgtac
    10261 aattccttcg gcacaccctg ctcgaggccc ggcacgccat cggggaaaac cggcttgcgc
    10321 tcgcccggca gcttcttctt ggtgtcgagg aaatcgagca aatcagtcgg atcccagttg
    10381 ccaaagccgc tcgagcagcc ggccaggacg cccgacagcg cgatgacgac agcggaggcg
    10441 atcagacgtt gcgagcggcg catggtcgat ttctcactca attttctcgg ccgtcagctt
    10501 ttcgcgaccg gcggaagcaa cgcctgcagc gcttcggcgc gcgagcgcag gcttggcggc
    10561 gtctcgctgt cattggcaat cagatccagc cactgccgcg ctgccgccgt gtcgttggcg
    10621 cgccaagccg acagcgcgag caattcgcgc gcggtatggc gaaaggtcgc tgccggcgcc
    10681 gtcgcaggct cgaggcgcga cagcatgttg ggataaccag acgtttccag cagcaactgg
    10741 gcggcgcgga tcttggcgag gtcctgctcc ggcgcaccga tgctgcgatc gttcgacatt
    10801 tcgtcgtaca gcttcaccgc cgcctgtgga tcacgg
//
LOCUS       DATNOI010000520         2696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1305375,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000520 DATNOI010000000
VERSION     DATNOI010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305375"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VN968_21010"
     CDS             complement(<1..1088)
                     /locus_tag="VN968_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HXL31751.1"
                     /translation="MTASIVGWAHTPFGKFDTETVESLVIRVANDALADAGISAADVD
                     EIVLGHFNAGFSPQDFTASLVLQADPQLRFKPATRVENACATGSAAVHQGIRAIAAGA
                     AKIVLVVGVEQMTRTPAPEIGRNLLRASYLPEDGETPGGFAGVFGKIAQAYFQKYGDQ
                     SDALAMIAAKNHKNGVANPYAQMRKDFGYEFCRSESEKNPFVAGPLKRTDCSLVSDGA
                     AALVLTDTETAKSMGKAVNIRATAHAQDFLPMSKRDILQFEGCTVAWQRALQSAGVQL
                     SDLSFVETHDCFTVAELIEYEAMGLTPKGQGARAIKEGWTQKDGKLPVNPSGGLKAKG
                     HPIGATGVSMHVMTAMQLLGQAPEGMQI"
     gene            complement(1120..2106)
                     /gene="denD"
                     /locus_tag="VN968_21015"
     CDS             complement(1120..2106)
                     /gene="denD"
                     /locus_tag="VN968_21015"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HXL31752.1"
                     /translation="MHILVLGAAGMVGRKLTERLLRDGRLGKRDISRMTLQDVVAPAK
                     PDEAGFPVDTVTCDFAVPGVAEKLVADRPDVIFHLAAIVSGEAELDFDKGYRINLDGT
                     RMLFDAIRLAGGGYKPRVVFTSSIAVFGAPFPEAIGDEFFHTPLLSYGTQKAIGELLL
                     ADYSRRGFMDGIGIRLPTICIRPGLPNKAASGFFSNILREPLAGKEAVLPVSEDVRHW
                     HASPRSAVGFLIRAATMDSEAAGARRNLTMPGLSATVGEEIAALKRVAGDKVAGRIKR
                     EPDPFIMGIVDGWPRNFEAKRAAKLGFTTAEKTFDDIIRIHIEDELGGKFVA"
     gene            complement(2151..>2696)
                     /locus_tag="VN968_21020"
     CDS             complement(2151..>2696)
                     /locus_tag="VN968_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL31753.1"
                     /translation="VRLRYRGAHWVGGLIFLSYLVPPSILFIPLATVVFQYGLFDSPM
                     ALILTYPTILIPFSTWLLMGYFKTIPFELEECALIDGASRWQILIKIVLPLAVPGLIS
                     AFIFCFTLCWNEFIYALTFLQSVQNKTVPVAIVNEFVDGDIYRWGSLMAGALAGSLPL
                     VILYAFFVEHYVSAMTGAVKE"
BASE COUNT          538 a          865 c          813 g          480 t
ORIGIN      
        1 ttgatctgca taccctcggg cgcctgaccc aacagttgca tcgcggtcat cacatgcatg
       61 gagacgccgg tggcgccgat tggatggcct tttgctttca acccgccgga gggattgacg
      121 ggcagcttgc cgtctttctg cgtccagcct tccttgatcg cacgcgcgcc ctgccctttc
      181 ggcgtcaacc ccatcgcttc atattcgatc agttcggcga cggtgaagca gtcgtgggtt
      241 tcgacaaagg aaagatcgga aagctgcaca ccggccgatt gcagcgcgcg ctgccatgcc
      301 acggtgcagc cttcgaactg caagatgtcg cgcttcgaca tcgggaggaa atcctgggca
      361 tgcgcggtcg ccctgatgtt gacggccttg cccatcgact ttgcggtctc cgtgtcggtc
      421 aagaccagcg ccgccgcgcc gtccgacacc agcgagcaat cggtgcgctt caatggacct
      481 gccacaaacg gattcttctc gctttcggag cggcagaatt catagccaaa atccttgcgc
      541 atctgcgcat agggattggc gacgccgttc ttgtggttct tggccgcgat catcgccagc
      601 gcatcggact ggtcgccgta tttctggaag taggcctgcg cgatcttgcc gaacacgccg
      661 gcgaaaccgc ccggggtctc gccatcctcc ggcagatacg atgcgcgcag caggttcctg
      721 ccgatctcgg gcgcgggcgt ccgcgtcatc tgctcgacac cgaccaccag cacgatcttg
      781 gccgcacccg cggcgatggc ccggatgccc tgatgcaccg ccgccgatcc cgtggcgcag
      841 gcgttttcga cccgcgtcgc cggcttgaac cgcaattgcg gatcggcctg cagcaccagc
      901 gaagcggtaa aatcctgcgg cgagaagccg gcgttgaagt gcccgagcac gatctcatcg
      961 acgtcggcag ccgaaatgcc ggcgtcggcg agcgcgtcgt tcgcgacgcg gatcacaagg
     1021 ctctcgacgg tttcggtatc gaacttgccg aatggcgtgt gcgcccatcc gacgatgctg
     1081 gctgtcatgg tcgtgctccc ttgagattac ggcaaacgat caggccacga acttaccacc
     1141 aagttcgtcc tcgatgtgaa tccggataat gtcgtcaaag gtcttttcgg cggtggtgaa
     1201 gccgagtttt gccgcccgct tcgcctcgaa attgcgcggc cagccatcga caatccccat
     1261 gatgaacgga tccggctcgc gcttgatccg ccccgccacc ttgtctcccg ccacccgctt
     1321 caaggccgcg atttcctcgc cgacggttgc cgaaaggccg ggcatggtga ggttgcggcg
     1381 cgcgccggcc gcctcgctat ccatggtcgc ggcacgaata aggaacccga ccgccgagcg
     1441 cggcgaggcg tgccaatggc ggacgtcctc ggacaccggc agcaccgctt ccttgccggc
     1501 gagcggttcg cgcaggatgt tggagaagaa tccggaagcc gccttgttgg gaagaccggg
     1561 acggatgcaa atggtcggga gccggatgcc aatgccgtcc ataaaaccgc ggcgcgagta
     1621 atcggccagc aaaagttcgc cgatcgcctt ctgggtgccg taactcaata gcggcgtgtg
     1681 aaagaactcg tcgccgatcg cttcgggaaa cggcgcgccg aacaccgcaa tcgacgaggt
     1741 gaacaccacc cgcggcttgt agccgccgcc ggcaagcctg atcgcatcga acagcatccg
     1801 cgtgccgtcg aggttgatcc gatagccctt gtcgaaatcg agctcggcct cgccggagac
     1861 gatagccgcc agatggaaga tcacatcggg ccgatccgcg accagctttt cggcgacgcc
     1921 cggaaccgcg aaatcgcagg tcaccgtatc gaccggaaat cccgcttcgt cgggctttgc
     1981 gggagcaacc acgtcctgca gcgtcatgcg gctgatatca cgcttgccga gacgtccgtc
     2041 gcgcaacagc cgctcggtca atttgcggcc gaccatgccg gccgcgccga gaaccaaaat
     2101 gtgcaagacc caactccttg ttcttgattc ttagacgcgg gcttgcgcgc tcactctttc
     2161 accgctccgg tcatcgccga gacgtagtgc tcgacaaaga acgcatagag gatcaccagc
     2221 ggcagcgagc ccgccagcgc gcccgccatc agcgatcccc agcgataaat gtcgccgtcg
     2281 acgaattcgt tgacgatcgc gaccggcacc gttttgttct ggaccgattg caggaaagtg
     2341 agcgcgtaga tgaactcgtt ccagcacagc gtgaagcaga agatgaaggc ggagatcaac
     2401 ccgggaaccg ccagcggcag cacaatcttg atcaggatct gccagcggct cgcgccatcg
     2461 atcagcgcac attcctccag ttcgaacggg atggtcttga aatagcccat caacagccag
     2521 gtcgagaacg ggatcaggat ggtcggatag gtcaggatca gcgccatcgg cgagtcgaac
     2581 aggccgtatt ggaacacgac ggtggcgagc ggaatgaaca ggatcgacgg cggcaccaga
     2641 taggaaagga aaatcaagcc gccgacccaa tgcgcgccac gatagcgcaa ccgcac
//
LOCUS       DATNOI010000521        11154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_917042,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000521 DATNOI010000000
VERSION     DATNOI010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11154
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917042"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /gene="pcaD"
                     /locus_tag="VN968_21025"
     CDS             complement(<1..713)
                     /gene="pcaD"
                     /locus_tag="VN968_21025"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HXL31754.1"
                     /translation="MPMIDADGCLLNVSVEGRDGGPTLMLSNSLGCTLQMWEPQMKAL
                     TQLFRVVRYDRRGHGKSAVPPGPYSMERFGRDVLAILDDLNIKKVHWCGLSMGGMVGQ
                     WLGANAPDRFDRIILSNTACHYPDPTNWLNRIKAVKEGGIAAVADTVIAGWLTADFRE
                     REPQITANMKAMLIASPVQGYLACCEALSTLDQRELLPRIKSPTLVIAGRHDVATPVA
                     AGEYIRSQIPGASLTILDAA"
     gene            complement(864..2219)
                     /locus_tag="VN968_21030"
     CDS             complement(864..2219)
                     /locus_tag="VN968_21030"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HXL31755.1"
                     /translation="MSTSLSPLLAPILSSAAMRRVCDDLTYLQHMLDFEAALARAEAA
                     TGVIPASAADTIAKACRAESFDLGALAEAATRSGNLAIPLVKALTAHVAKADPDAARY
                     VHWGATSQDVIDTATMLGLRAAIDALLSDTGRAIAGFAKLAAKYRDTAMVARTWLQHA
                     LPMPFGLKLAEYAAALHRSRARLQRLRSEALALQFGGAAGTLAALGDKGWLVAERLAL
                     ELELPLPDAPWHTHRDRIAEAASVFAILAGICGKIARDLQLMMQTDVAEAFEPSGEGR
                     GGSSTMPHKRNPVAAATALAAATMAPGLAATIFAAQVQDHERSAGPWHAEWPTLPMLL
                     LVTSGALAAIVDIAEGLEVDAVRMRVNLEATHGLIMAEAVTMALAETIGKSEAHHLIE
                     AASKKAIADKKHLRDVLAKDPKVTAHLNADKLTKLFEPMAYQGVSQALIDRLLASLDK
                     K"
     gene            2443..2889
                     /locus_tag="VN968_21035"
     CDS             2443..2889
                     /locus_tag="VN968_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HXL31756.1"
                     /translation="MAMTMNGEVQLAAPREAVWTKLNDPEVLKACIPGCEELEKTEDN
                     GFRAVAKMKVGPVSARFKGRVTLSDLDPPNGYKISGEGEGGVAGFAKGGATVGLAEKD
                     GGTLLTYNVEAQIGGKLAQLGQRLINGAAKKLADEFFANFAKAVQG"
     gene            3051..3536
                     /locus_tag="VN968_21040"
     CDS             3051..3536
                     /locus_tag="VN968_21040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL31757.1"
                     /translation="MAKISLIVNGNPVNANVDPRTLLVQFLRENLRLTGTHVGCDTSQ
                     CGACVVHLDGKAVKSCTTLAVMADGHEVKTIEGLAADGAPLHPMQEAFREHHGLQCGF
                     CTPGMIMTAVDIVHRKGHDLSDHTIREELEGNLCRCTGYQNIVQSIVAGAKAMANSDL
                     A"
     gene            3566..4372
                     /locus_tag="VN968_21045"
     CDS             3566..4372
                     /locus_tag="VN968_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXL31758.1"
                     /translation="MYEFKYHRPATVRQAANLLIKNEDAKVIAGGHTLLPVMKQRLAS
                     PPHLVDLSHIEGLDGIEMKGRALVIGATARHADVANSAIVGESIPALAELAGLIGDPA
                     VRHKGTIGGSLANNDPTADYPAACLALGATIVTNKRRLKPEEFFQGLFTTSLESDEII
                     TKVMFPVPKKAAYIKFRNQASRYALVGVFVAKRPSDVRVAVTGAGSEGVFRVAAFEEA
                     LKKRFSPKALDGLSVPADGLNSDLHGSAEYRAHLIGVLARRAVEAATGKA"
     gene            4410..5318
                     /locus_tag="VN968_21050"
     CDS             4410..5318
                     /locus_tag="VN968_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXL31759.1"
                     /translation="MTASALPTSVDAMLELLTSRGYLAERALATVTYLSLRMGRPLFL
                     EGEAGVGKTEIAKVLSAALGRKLIRLQCYEGLDVASAVYEWNSAAQMIAIRLAEAAGD
                     TDRDQLSSDIFAERYLIKRPLLQALEPDVAGAPVLLIDELDRADEAFEAYLLEILSDF
                     QVTIPELGTVKAPHPPIVIVTSNRTREIHDALKRRCLYHWVDYPSAERELAIVKSRVP
                     GISAKLSQQVVHFVQALREQDFYKSPGVAETIDWATALTELDARSLTPQVVGDTLGAL
                     LKYQDDIARMQGDTLQKVLKEATSEN"
     gene            5431..6630
                     /locus_tag="VN968_21055"
     CDS             5431..6630
                     /locus_tag="VN968_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXL31760.1"
                     /translation="MAINHLSPPTGQMADNVIGFARALRAAGIPVGPGAVIDALNALQ
                     VIEIGNRADVFTTLEAIFVKRHEHALIFAQAFDLFFRAAEDWKHMLDSVPLPDHARKK
                     PPPASRRVQEALSQPSMSETPQAQEQELRLSVSDKEVLQKKDFAQMSAAEIAQVTRAI
                     AQMKLPQAQLRTRRYQPDVRGLRLDMRRTLRGSLRTGGDIIDIHRLGRIDKPAPIVAL
                     LDISGSMSEYTRLFLHFLHAITDARKRVSVFLFGTRLTNVTRSLRARDPDEALASCSS
                     TVEDWAGGTRIATSLHTFNKLWGRRVLGQGAIVLLISDGLEREADARLAFEMDRLHRS
                     CRRLIWLNPLLRYGGFEAKAQGIKMMLPHVDEFRPVHNLSSIEGLIAALSSVLPPHHV
                     SHIRPAA"
     gene            6643..6972
                     /locus_tag="VN968_21060"
     CDS             6643..6972
                     /locus_tag="VN968_21060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HXL31761.1"
                     /translation="MLNRDEDILRAAEDWQKAGHGVALATVVETWGSAPRPAGSSLVI
                     NDDGTFLGSVSGGCVEGAVVTEALDVIASGQPKMLEFGVADETAWNVGLSCGGTIRVF
                     VEKVGQS"
     gene            6969..7670
                     /locus_tag="VN968_21065"
     CDS             6969..7670
                     /locus_tag="VN968_21065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HXL31762.1"
                     /translation="MKLETLTEVNVERAARRPVIVVTDTANGEQRLVKAADIATDPLR
                     ADLAKQLRMGKSGMVEAGGKKLFLNVYAPTARLVIIGAVHISQALAPLARALDYDVTV
                     VDPRTAFASPERFPDVPLIAEWPDVALPPLKIDHYTAFVALTHDPKIDDPALLHAFER
                     DCFYIGALGSRKTHAKRGERLQAQGARDSDIARIHAPIGLPIGAVSPSEIAVAIMAEI
                     TAQLRLPAKEKEAAA"
     gene            7667..9271
                     /locus_tag="VN968_21070"
     CDS             7667..9271
                     /locus_tag="VN968_21070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding/glycosyltransferase family
                     2 protein"
                     /protein_id="HXL31763.1"
                     /translation="MKFGPASPAEAIGGVTVHTLRQGSLVLKKGTTIGPAEVAQLQRA
                     GVKEVVVVRLEDGDVSEDVAAASIAEAVAGDGIHVERAFTGRANLFAAKAGVLVVDRA
                     AVDRINGVDEAITFATLTAYKPVVEGEMVATVKLIPFGVEAKLRDAAVKAASGGALRI
                     APYTIKRVGIVSTLLPGLAPKVVEKTLRVTAERLAPAGATIIAERRVPHDETALAAAI
                     QELLGLGADLVIVFGASAIADRRDVIPAAITEIGGAIEHFGMPVDPGNLLLIGSAGGV
                     PVLGAPGCARSPVENGFDWVLMRLLAGLKVTRAELTGMGVGGLLMEIVTRPQPREKPG
                     TDGNRNVAAIILAAGRSTRMGGPNKLLAELGGKTLVRIVTEQALASKAKGVIVVTGHQ
                     AEQVEKALQGLNVKFVRNPDFAQGLASSVKAGVAAVPGDADGAVICLGDMPLIDAHLI
                     DRLIEAFAPDRGNLIAVPVSDNRRGNPVLWSRRFFNELMTLDGDIGARHLIARHSEAV
                     AEVPVEGHGAFLDIDTPQALAAAQRG"
     gene            9412..9762
                     /locus_tag="VN968_21075"
     CDS             9412..9762
                     /locus_tag="VN968_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HXL31764.1"
                     /translation="MLVLTLALAGSRFITETRAAGAFAIGKCGAYGQAYDYAGEKVAR
                     AEALKHCRGQCTTVTMKRACAALAVDMANPCGAHGYAVKPRISSSLNEATRKCYASGG
                     KECVIRAWACDAKG"
     gene            9898..10308
                     /locus_tag="VN968_21080"
     CDS             9898..10308
                     /locus_tag="VN968_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 family protein"
                     /protein_id="HXL31765.1"
                     /translation="MQFDTKIAVVIRSDLEAWQKLNVASFLAGGIAAAFPECIGEPYE
                     DGSGTKYLSLIGQPILIYRADRMALTRALERALARNAKPAVYTEDMFKTTHDAANREV
                     VKAVIRGELNLVGLAMRAERKVIGKIVDGLKFHS"
     gene            10844..>11154
                     /locus_tag="VN968_21085"
     CDS             10844..>11154
                     /locus_tag="VN968_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HXL31766.1"
                     /translation="MKLIPTRQRLLLALAAIAVAYLMLAYVLLPVLWTHHEHEPGLAL
                     LPMVTRTSAGIPGDALNVGLVGVKEDVLRAMHEAGWFPADPITLRTSIEIVGSVVLDR
                     P"
BASE COUNT         2008 a         3541 c         3642 g         1963 t
ORIGIN      
        1 gcggcgtcga gaatggtgag gctggcgccg ggaatctggc tgcggatgta ctcgccggcc
       61 gcgaccggcg tcgccacgtc gtggcggccg gcgatcacca gcgtcgggct tttgatcctg
      121 ggcaagagct cgcgctggtc gagcgtgctc agggcttcgc agcaggcgag atagccctgc
      181 accggcgaag cgatcagcat cgccttcata ttcgcggtga tctgcggctc gcgctcgcgg
      241 aaatccgccg tcagccagcc cgcgatgacg gtgtcggcca ccgctgcgat gccgccttcc
      301 ttcaccgcct tgatgcgatt gagccagttg gtcggatcgg gatagtggca agcggtgttg
      361 gagagaatga tccggtcgaa gcgatcgggc gcgttggcgc cgagccactg tccgaccatg
      421 ccgcccatcg acaaaccgca ccaatgtacc ttcttgatgt tgaggtcatc gaggattgcc
      481 agtacatcgc ggccgaagcg ttccatggaa taggggccgg gtgggacggc cgatttgcca
      541 tggccgcgcc ggtcatagcg gaccacgcga aacagctggg tgagcgcctt catctgcggc
      601 tcccacatct gcaaggtgca gcccagcgaa ttcgacagca tcagggtcgg cccgccgtcg
      661 cggccttcga cggacacgtt gagcaggcaa ccgtcggcat cgatcatcgg cattgtgggt
      721 tcctcatcag cgcgtgctaa agcgcgaagc gcgtcttcag cgcgaagcgc gtcttcgcac
      781 tggctgatcc gcacatccat ctagaaaaga acatctatcc aagaagatgg attgccgggt
      841 caagcccggc aacgacatct ggactacttt ttgtcgagcg aagcgagcag ccggtcgatc
      901 agggcttgcg aaacgccctg ataggccatc ggctcgaaca gttttgtcag tttgtctgca
      961 ttcagatgcg cggtgacctt tgggtctttt gccaggacgt cacgcaggtg ttttttgtcg
     1021 gcgatggcct tcttgctcgc ggcttcgatc agatgatggg cctcgctctt gccgatcgtc
     1081 tcggccagcg ccatcgtcac cgcttcggcc atgatcagcc cgtgggtggc ctcgagattg
     1141 acgcgcatgc gcaccgcatc gacttccagc ccttcggcga tatcgacgat cgcggcgagc
     1201 gcgcctgagg tgaccagcag cagcatcggc aaggtcggcc attccgcgtg ccacggcccg
     1261 gcgctgcgtt cgtggtcctg cacttgcgcc gcgaaaatcg tcgccgccag accgggcgcc
     1321 atggtggcgg ctgcgagcgc ggtcgcggca gcgaccgggt tgcgcttatg cggcatggtc
     1381 gaagaaccgc cgcggccttc gcccgacggc tcgaacgcct cggcgacgtc ggtctgcatc
     1441 atcagttgca ggtcgcgggc gatcttgccg catatgcccg caaggatcgc gaacacggaa
     1501 gcggcctccg cgatgcggtc gcggtgggta tgccagggcg catcaggcag cggcagttca
     1561 agctccagcg ccagtctttc tgccaccagc catcccttgt cgccgagcgc ggcgagcgtg
     1621 ccggcggcgc cgccgaactg cagcgccagt gcctcgctgc gcaatcgttg caaccgtgcg
     1681 cgcgagcgat gcaacgccgc ggcatattcc gcgagtttca atccgaacgg catcggcaag
     1741 gcatgctgca gccaggtgcg cgccaccatc gccgtgtcgc gatacttcgc tgcgagtttg
     1801 gcaaagcccg cgatggcgcg gccggtatcg gagagcagcg cgtcgatggc ggcgcgcaag
     1861 ccgagcatgg tcgcggtatc gatcacgtcc tggctggtgg cgccccagtg cacgtagcgc
     1921 gcggcgtccg gatctgcctt ggcgacgtga gcggtgagcg ccttgaccag aggtattgcg
     1981 agattgccgg agcgcgtcgc ggcctcagcc agggcgccaa ggtcaaacga ttcggccctg
     2041 caggccttcg cgattgtatc cgccgcgctt gcgggaatga cgccggttgc cgcctcggcg
     2101 cgcgcaaggg cggcttcaaa atcaagcatg tgctggagat acgtcaggtc gtcgcagacc
     2161 ctgcgcatgg cggcgctcga cagaatgggc gcgagcaaag gcgagagtga agtgctcatg
     2221 atacgcacga cctaaccatc cccgcctgcc gctgccaatc ccaaagcgca aatcccaagg
     2281 tacaaatccc aaggcacaaa tcccaaggtg caaatcccaa ggcgcccgca ggcctgctgc
     2341 gctgcgaata tgcatcgctc atgaccgcat catccccttc cttttggggc tcgcgtgctt
     2401 tacttggggg ccagaccacg cgcaatccgg gaggcaccca cgatggccat gacgatgaac
     2461 ggcgaggtcc agcttgcggc gccgcgcgag gcggtgtgga ccaaattgaa cgatccggaa
     2521 gtgctcaagg cctgcatccc cggttgcgag gagctggaga agaccgagga caacggtttc
     2581 cgcgcggtgg ccaagatgaa ggtcgggccg gtgtcggccc ggttcaaggg gcgcgtgacg
     2641 ctgagcgatc tcgatccgcc caacggctac aagatatcgg gtgaaggcga gggcggtgtc
     2701 gccggcttcg ccaagggcgg cgcgacggtg gggttggccg agaaggacgg cggtacgctg
     2761 ttgacctaca atgtcgaggc gcagatcggt ggcaagctcg cccagctcgg gcagcggctg
     2821 atcaacggtg cagccaagaa actggccgac gaattttttg cgaatttcgc caaggcggta
     2881 caaggctgat tccccgagga ggctagttcc tcggaggacc gccgaaccat accaggccat
     2941 gccccaaggt gaggttgctc attgccggga tggcccatat tatggcgctg gaatgatttt
     3001 aatgactgtg ctgccgtgca atgcagcagc gcagctgaag agagtgctga atggccaaga
     3061 tttccctgat cgtgaacggc aatcccgtca acgccaatgt cgacccccgc accttgctgg
     3121 tgcagttcct gcgcgagaat ctgcggctga ccggcaccca cgtcggctgc gacacctcgc
     3181 aatgcggcgc ctgcgtcgtg catctcgacg gcaaggcggt caaatcctgc accacactcg
     3241 cggtcatggc tgacgggcat gaggtcaaga ccatcgaagg ccttgccgct gacggcgcgc
     3301 cgctgcatcc gatgcaggaa gcgtttcgcg aacaccacgg gctgcaatgt ggcttctgca
     3361 cccccggcat gatcatgacc gcggtcgaca tcgttcaccg caagggccat gatctcagcg
     3421 accacaccat ccgcgaagag ctcgaaggca atctctgccg ctgcaccggc taccagaaca
     3481 tcgtccaatc gatcgtggcg ggcgccaagg cgatggcgaa ctccgacctc gcgtaacgtc
     3541 atcgcacggc gatcaggaac acttcatgta cgaattcaaa tatcatcgtc cggcgactgt
     3601 acggcaggcc gccaatctct tgatcaagaa tgaagacgcc aaggtgatcg ccggcggcca
     3661 taccttgttg ccggtcatga agcagcggct cgccagcccg ccgcatctgg tcgacctctc
     3721 ccacatcgag gggctggacg gcatcgaaat gaagggccgc gcgctggtca tcggcgccac
     3781 tgcaagacat gccgatgtgg ccaattcggc catcgtcggc gagtccatcc cggcgctggc
     3841 cgaactcgcc ggcttgatcg gcgatcccgc ggtgcgccac aagggcacca tcggcggctc
     3901 gctcgccaac aacgatccca ccgccgatta tcccgcggct tgcctggcgc tcggcgccac
     3961 catcgttacc aacaagcgcc gcctgaagcc ggaagagttc ttccaggggc tgttcacgac
     4021 gtcgctcgag agcgacgaga tcatcaccaa ggtgatgttt ccggtgccga agaaggcggc
     4081 ctacatcaaa ttccgcaacc aggcctcgcg ctatgcgttg gtcggggtgt tcgtcgccaa
     4141 gcgcccgtcg gacgtgcgcg ttgccgtcac cggcgccggc tccgaaggcg tgttccgtgt
     4201 cgccgcgttc gaggaagcgt tgaagaagcg cttctcgccg aaggcgcttg acggtctgtc
     4261 ggtgcctgcc gacgggctga acagcgacct gcacggcagt gccgaatacc gggcgcatct
     4321 gatcggggtg ctggcgcggc gcgccgtcga agccgccacc ggcaaagcct gacattgcca
     4381 attccgttga catcaagact ggctttccca tgactgcatc ggcgttgccc acctccgtcg
     4441 atgcgatgct cgaacttctg acctcgcgcg gctaccttgc cgaacgcgcg ctggcgacgg
     4501 tgacctatct gtcgctgcgc atgggccggc cgctgtttct cgaaggcgag gccggcgtcg
     4561 gcaagaccga gatcgccaag gtattgtcgg ccgcattggg gcgaaaactg atccgcctgc
     4621 agtgctacga aggactggac gtcgcctccg cggtctatga gtggaacagt gccgcgcaaa
     4681 tgatcgcgat ccggctcgcc gaggcggccg gcgataccga tcgcgaccag ctttcctctg
     4741 atatcttcgc cgagcgctac ctgatcaaac ggccgctgct gcaggcgctg gagccggatg
     4801 tggccggcgc cccggtgctt ctgatcgacg aactcgaccg cgccgacgag gcgttcgagg
     4861 cctatctctt ggaaattctc tctgacttcc aggtgacgat ccccgaactc ggcaccgtga
     4921 aggcgccgca tccgccgatc gtcatcgtca cctcgaaccg cacccgcgaa atccacgacg
     4981 cgctgaagcg ccgctgcctc tatcactggg tggattatcc cagtgccgag cgtgagctcg
     5041 cgatcgtcaa atcgcgcgtg ccggggatct ccgcgaaatt gtcgcagcag gtcgtccact
     5101 tcgtgcaggc gctgcgcgag caggattttt acaagtcgcc cggggtcgcc gagaccatcg
     5161 actgggccac cgcgctgacc gaactcgacg cccgctcgct gaccccgcag gtcgtcggtg
     5221 atacgctcgg cgcgctgttg aagtaccagg acgacatcgc gcggatgcag ggcgacacgc
     5281 tgcagaaggt tttgaaggaa gcgaccagcg agaattagca acccccgtca ttccggggcg
     5341 tcgcgcagcg acgaacccgg aatctcgcgt tacaatctct ggattccggg ttcgcgctgc
     5401 gcgcgccccg gaatgacgac ggtaggtccg atggccatca atcatctctc gcccccaacc
     5461 ggccaaatgg cggacaacgt cataggcttt gcccgcgcgc tgcgcgccgc cgggattccg
     5521 gtcgggccgg gtgcggtgat cgatgccttg aacgcgctgc aggtgatcga gatcggcaat
     5581 cgcgccgatg tattcacgac gctggaggcc atcttcgtca agcgtcatga gcatgcgctg
     5641 attttcgcgc aggcgttcga tctgttcttc cgcgccgccg aggactggaa gcacatgctg
     5701 gattcggtgc cgctgccgga tcacgccagg aaaaagccgc cgccggcctc gcgccgggtc
     5761 caggaggcgc tgtcgcagcc ttcgatgagc gaaacgccgc aggcccagga gcaggagctc
     5821 cggctgtcgg tgtcggacaa ggaagtgctg cagaagaagg atttcgcgca gatgagtgcg
     5881 gccgagatcg cacaggtcac ccgcgcgatt gcgcagatga aactgccgca ggcccaattg
     5941 cgcacccgcc gctatcagcc ggacgtgcgg ggcttgcggc tcgacatgcg ccgtaccctg
     6001 cgcgggtcgt tgcgcaccgg cggcgacatc atcgacatcc atcgcctcgg gcggatcgac
     6061 aagccggcgc cgatcgtggc gctgctcgat atatcgggat cgatgagcga atatacccgg
     6121 ctgttcctgc atttcctgca tgccatcacc gatgcgcgca agcgggtttc ggtgtttctg
     6181 ttcggcaccc ggctcaccaa tgtgacgcgc agcttgcggg cgcgggatcc cgacgaggcg
     6241 ctggcgagct gttcgtcgac ggtggaggac tgggccgggg gcacccgcat cgccacctcg
     6301 ctgcatacgt tcaacaaatt gtggggccgg cgcgtgctcg ggcagggcgc gatcgtgctg
     6361 ttgatttccg acgggctgga gcgcgaggcc gacgccaggc tggcgttcga gatggaccgg
     6421 ctgcaccggt cctgccgccg cctgatctgg ctcaatccgc tgctgcgcta tggcgggttc
     6481 gaggccaagg cgcagggcat caaaatgatg ctgccgcacg ttgacgaatt ccgcccggta
     6541 cataatttga gttcaatcga ggggctgatc gcggcacttt cgtccgtcct gccgccgcac
     6601 catgtcagcc acatccgtcc cgcagcttga aagaggctcc ccatgctcaa tcgcgacgaa
     6661 gacattctca gggcggctga ggactggcag aaggccggcc acggcgtagc gctcgccacc
     6721 gtggtcgaga cctggggctc ggcgccgcgg cccgccggct ccagcctcgt gatcaacgat
     6781 gacggcacct tcctgggctc ggtttccggc ggctgcgtcg aaggcgcggt cgtcaccgaa
     6841 gcgctcgatg tgatcgccag cggccagccg aagatgctgg aatttggcgt cgccgacgaa
     6901 accgcctgga atgtcgggct gtcctgcggc ggcaccatcc gggtgttcgt cgagaaggtc
     6961 ggccagtcgt gaagcttgag acgctgacag aggtcaatgt cgagcgcgcc gcgcggcgtc
     7021 cggtgatcgt ggtcaccgat accgccaatg gcgagcagcg gctggtcaag gccgccgaca
     7081 tcgctaccga cccgttgcgc gccgatctcg ccaagcagtt gcggatgggc aagagcggca
     7141 tggtcgaggc cggcggcaaa aagctgtttc tcaacgtcta tgcgccgacc gccaggctcg
     7201 tcatcatcgg cgcggttcat atcagccagg cgctggcgcc gctggcgcgc gcgctggact
     7261 atgacgtgac ggtggtggat ccgcgcaccg cgtttgccag tcccgagcgg tttcccgatg
     7321 tgccgctgat cgcggaatgg cccgatgtgg cgctgccgcc gctcaagatc gatcactaca
     7381 cggcgttcgt ggcgctgacg cacgatccca agatcgacga tccggcgctg ctgcatgcgt
     7441 tcgaacgcga ttgcttctat atcggcgctc tgggctcgcg aaagacccac gccaagcgtg
     7501 gcgagcggct gcaggcgcaa ggcgccaggg atagcgatat cgcccgcatt cacgcgccga
     7561 ttggcctgcc gattggcgcg gtctcgccgt ccgagatcgc ggtggcgatc atggccgaga
     7621 tcaccgcgca gctgcggctg cccgcgaaag aaaaagaagc cgcggcatga aattcggtcc
     7681 tgccagcccg gcggaagcga tcggcggcgt caccgttcac accctgcgcc agggatcgct
     7741 ggtgctcaag aagggtacta cgatcggtcc ggccgaggtc gcgcagctcc agcgcgccgg
     7801 cgtcaaggag gtcgtcgtgg tccggctcga ggacggtgac gtctccgagg acgtcgcggc
     7861 cgccagcatc gccgaagccg tcgccggtga cggcatccat gtcgagcgcg cctttaccgg
     7921 ccgcgccaac ctgttcgccg ccaaggccgg cgtgctggtg gtggaccgcg ccgccgtcga
     7981 ccgcatcaac ggcgtcgatg aagccatcac cttcgccacg ctgaccgcct acaagccggt
     8041 ggtcgaaggc gagatggtgg cgaccgtcaa gctcattccg ttcggggtcg aagcaaaact
     8101 aagggatgcc gcggtcaagg cagccagcgg cggcgcattg cggatcgcgc cctacaccat
     8161 caagcgcgtc ggcatcgtct cgaccttgct gccgggactg gcgccgaagg tggtggagaa
     8221 aaccctgcgg gtcacggcgg agcggctggc gcccgcaggc gcgaccatta tcgccgaacg
     8281 ccgcgtgccg catgatgaga ccgcgctggc ggcggcgatc caggaattgc tcggtctcgg
     8341 ggcggacttg gtgatcgtgt tcggggcgtc ggcgatcgcc gaccggcgcg atgtgattcc
     8401 cgcggccatc acggagatcg gcggcgccat cgaacatttc ggcatgccgg tcgatcccgg
     8461 caatctcctg ctgatcggca gcgccggcgg cgtgccggtg ctgggcgcgc cgggctgcgc
     8521 acgctcgccc gtggagaatg gttttgattg ggtgctgatg cgtcttctgg cagggctcaa
     8581 ggtcacccgc gccgaactca ccggcatggg cgtcggcggg ctcttgatgg aaatcgtcac
     8641 ccggccgcag ccgcgcgaaa agcccggcac cgacggcaac cgcaatgtcg cggccatcat
     8701 tctcgcggcc gggcgctcga cccggatggg tggccccaac aagctgttgg ccgaactcgg
     8761 cggcaagacg ctggttcgga tcgtgaccga gcaggcattg gcctcgaaag cgaaaggcgt
     8821 gattgtcgtc accggccacc aggccgagca ggtcgagaaa gcgctgcagg gactgaacgt
     8881 aaaattcgtg cgcaatccgg atttcgcgca gggacttgcc agctcggtca aggccggcgt
     8941 cgcggcggtg ccaggcgacg ccgatggcgc cgtgatctgt ctcggcgaca tgccgctgat
     9001 cgatgcccat ctgatcgacc ggctgatcga agcgttcgcg ccggaccgcg gcaatttgat
     9061 cgcggtcccc gtcagcgaca acaggcgcgg caatccggta ttgtggtcgc gccgcttctt
     9121 caacgagttg atgacgctgg acggcgacat cggcgcccgg catttgatcg ccaggcacag
     9181 cgaggcggta gcggaagtcc cggtcgaggg ccacggcgcc ttcctcgata tcgacacgcc
     9241 gcaggcgctc gcggccgcgc agcgggggta aaatccgtta ccaggctcgt gaacgtccat
     9301 tttcaaactt cgttcaccaa gtcgaaatat cccccgcgta gattggcgcc ggctgacctc
     9361 ggggagggga tcttgacgtc atttgcaaga gcgcaacgtt gcgcgaggtt tgtgctggtt
     9421 ctgactttgg cattggccgg atcgcgcttc atcacggaaa cacgggcggc gggcgcgttc
     9481 gcgatcggca aatgcggcgc ctacggccag gcctacgact atgcgggcga gaaggtggcg
     9541 cgcgccgagg cgctcaagca ctgcaggggc cagtgcacca cggtgaccat gaagcgcgcc
     9601 tgcgccgcgc tggcggtcga catggccaat ccctgcggcg cccacggcta cgcggtcaag
     9661 ccaaggattt ccagttcgct caacgaggcg acccgcaaat gctacgcatc cggcggcaag
     9721 gaatgcgtga tccgcgcctg ggcctgcgac gccaaggggt agattttcaa ctatagtcat
     9781 tccggggcgc cgcgcagcgg cgaacctcag atgcgcaatt gcacattggg gaatctcgag
     9841 attccgggtc tgcgctaacg gcgcatcccg gaatgacgct gtctgaccgg agccatcatg
     9901 caattcgata ccaagatcgc ggtcgtgatc cggtccgacc tcgaagcgtg gcagaagctc
     9961 aacgtcgcgt cgttcctcgc cggcggcatt gccgcggcgt ttccggaatg catcggcgaa
    10021 ccctacgagg atggctcggg cacaaaatat ctgtcgctga tcggccagcc gatcctgatc
    10081 tatcgcgctg accggatggc gctgacccgc gcgctcgaac gggcgctggc gcgcaatgcg
    10141 aagcccgccg tctataccga ggacatgttc aagaccacgc acgacgcggc caaccgggaa
    10201 gtggtgaagg ccgtgatccg cggcgagctc aatctggtcg gcctcgccat gcgggccgag
    10261 cgcaaggtga tcggcaagat cgtcgacggg ctgaagtttc atagctagcg tttttgcctg
    10321 tcgcgccgct caggtcttcg ccgaagcaaa ggctggttcg agccgctgga cggtcataag
    10381 cccgtcgcga agcaatattc gtgccccgga tcgcgaccgg tagggccagg gcgcgtattc
    10441 atgaatcgaa cttgattggt gcaggcttga ccaatgtccg ctatgccccg gtagcgaaca
    10501 cattccgcac cgcaacgacc tgacctattt atgacgtcga ggcgccggcg ccggcggtcc
    10561 caagtcgcgc tgccagatcg ttgcgctatc aaactgaatg agcattccgt ccggtgaata
    10621 gacggcaccc tggttctgga catcgatcca gatccgcgtt gccggtgaaa accaatccgg
    10681 ccatgcgcgg gcgggctcac cagcttcgtt gaatggattc agatcgggac cgttctgggt
    10741 gatataggcg ggatagtcta cgagacccga acgacacatt tgaacgcata tgtacgtgcc
    10801 ggtcagattg actggttgcg tcgacgccgc gggagcgatt tgaatgaaac tgatcccaac
    10861 cagacaacga ttgctcctcg ctctagcggc cattgcggtc gcttatctca tgctggctta
    10921 cgtcctgctc cctgtcttgt ggacccatca tgagcatgaa cccggcctgg ccttgctgcc
    10981 aatggttaca cgcaccagcg ccggaattcc gggagatgca cttaacgtcg gactcgttgg
    11041 cgtcaaggaa gacgtgcttc gcgccatgca cgaggcgggc tggtttcctg ccgatccgat
    11101 cacgttgcgc acgagcatcg agatcgtcgg cagcgtggtg cttgaccggc cata
//
LOCUS       DATNOI010000522        15882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_392327,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000522 DATNOI010000000
VERSION     DATNOI010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15882
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392327"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VN968_21090"
     CDS             complement(<1..270)
                     /locus_tag="VN968_21090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31767.1"
                     /translation="MNLRLYLCAIAAALSLDVEAAWAQLSGHGGPVRALAISADGKTV
                     ISGSFDTSAIRWSLATDSAEQVLRFHSDAVNAVVFLQDGRLATAGA"
     gene            1544..1723
                     /locus_tag="VN968_21095"
     CDS             1544..1723
                     /locus_tag="VN968_21095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31768.1"
                     /translation="MLRDAKDAPLEGLGWIHGFIPSSPLLFKRAVRRPELRKIARLAN
                     TTAFEHDDLTRALAL"
     gene            complement(1878..2684)
                     /locus_tag="VN968_21100"
     CDS             complement(1878..2684)
                     /locus_tag="VN968_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL31769.1"
                     /translation="MSRSQLVAEHLPLLRRYARALTGNQASGDAYVGAMLEALLQDPS
                     LLDEAHGPRAGLFRLFTQIWNSVSLNDDTDADVTTLPMPSERRLSNITPLPRQAFLLL
                     SLEGFSEEEVAFVLGIEVAETRQLADTAGREMAAEIATDVLIIEDETFIAMDLESLVK
                     NLGHNVIGVARTHSDAVALAKNRKPGLILADIQLADGSSGLDAVNELLRIFEVPVVFI
                     TAYPERFLTGERPEPAFLISKPFQPAMVSAVASQALFFQRNSRNRAPRVA"
     gene            2974..3180
                     /locus_tag="VN968_21105"
     CDS             2974..3180
                     /locus_tag="VN968_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HXL31770.1"
                     /translation="MKEIKKPAKPGGLNAEIQSRIGHQLRAMYDDVVRQGVPDRFAEL
                     IRKLDTQEANPQLGTAGEKTEGSD"
     gene            3180..3728
                     /locus_tag="VN968_21110"
     CDS             3180..3728
                     /locus_tag="VN968_21110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXL31771.1"
                     /translation="MPLTDALRDDILASVPSLRAFAISLSGNGDRADDLVQETLLRAL
                     ANIDSFQPGSNLPAWLFTILRNLFRSDYRKRRREVEDADGSYAKTLKTQPAQNAHLEF
                     EEFRAALEKLPQDQREALILVGASGFSYEDAAAICGCAVGTIKSRVNRARSKLSALLY
                     VDGTDDFGPDETVRAVIGGSGG"
     gene            complement(4530..6188)
                     /locus_tag="VN968_21115"
     CDS             complement(4530..6188)
                     /locus_tag="VN968_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HXL31772.1"
                     /translation="MVRLGFIIGFIALIGALLSGLAAYRVHDQELTVDGIALARAIDV
                     HASLVQDRLTERELLARVASGLFRAPSVVKANMLQPLRSSIYAFKTDFIVASWIARLK
                     PNELDAARKELATAGFSNPAIRGFDDQPLNTRSLDKPIDVLMDVEPRDAETLRFLGRS
                     YDRDPVLGPTLAQAMADGKPVASDPAPLLRPDGQIGLVLATPVLQETDASPAGFVTFS
                     YELAPLMLANDEMSLFSVVLKDPRHAAGELVSDDRGIVTSRTVAPEAPTPSATRTVAF
                     GGRDWGLVYYAKTNSLKRAQQTAAIVAAIGLALTGIVCGLFGYVAYNNLRLSREIQVR
                     IGFERRLTAVIDELNHRVKNILAVIQSIVTRTLRHGSDIDVARDLLIGRIHAMSNVVS
                     LLSESQWQGVKLKGLFEARAIPHAERIAVSGPDVAVSARAAQSLSLLFFELASHSDEG
                     LSLVGKHPHIVAHWEVTGEEPDTTFHFRWEEFNTSAATRREDSDFGVILLDRVAPEAL
                     GGVSKRYFTEVSYVYELTAPMQTVVDLTERDRTEQLSAPPRPGR"
     gene            complement(6508..7146)
                     /gene="ureG"
                     /locus_tag="VN968_21120"
     CDS             complement(6508..7146)
                     /gene="ureG"
                     /locus_tag="VN968_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HXL31773.1"
                     /translation="MSKTNGPLRIGIGGPVGSGKTALMDLLCKAMRERYDIAAITNDI
                     YTKWDAEFLVRSGSLTPDRIAGVETGGCPHTAIREDASMNLAAVADMRAKFPDLDMVL
                     IESGGDNLAATFSPELADLTIYVIDVAAGDKIPSKGGPGITRSDLLVINKIDLAPHVG
                     ASLEKMAADARRMRGERPFVMTNLRKSEGLDRLIGFIEAKGGLSSPAQAKVG"
     gene            complement(7229..7966)
                     /locus_tag="VN968_21125"
     CDS             complement(7229..7966)
                     /locus_tag="VN968_21125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HXL31774.1"
                     /translation="MLMTTSKAPPTNDRSGMKQGEAAALYRLMTWLSPAFPVGAFSYS
                     SGIEWAVEAGDIVDAASLRDWLASMLADGTGFCDGVLLCHAHRAASSHDEAGLRETAE
                     LAAAFVASRERQLETTTQGRAFIEIARSAWNCDGLDAMISRCDGAIVYPVAVGLVSAA
                     HAIPLAPTMHAFLHALTSNWISAGARLIPLGQTDSQRVLALLEPIVVATGQRALDASL
                     DDLGSATFRADLAGMRHETQYTRLFRS"
     gene            complement(7947..8543)
                     /gene="ureE"
                     /locus_tag="VN968_21130"
     CDS             complement(7947..8543)
                     /gene="ureE"
                     /locus_tag="VN968_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HXL31775.1"
                     /translation="MIRATRVRGAHRWTEAPADTVVLDFDDRHRRRMAMTGTRGLEFL
                     LDLENAVALRGGDALVLDDNRLIEVVAAPESLLEIRGQDPQHLVRVAWHLGNRHLPTQ
                     IMPKSLRIRRDHVIEAMVKGLGARVIEIEAPFDPEGGAYAGGGHAHAAESDPHGHAAH
                     DGPSHEHGEHHHHGDHHHHDEHCDHDHHHGHSHAHDHK"
     gene            complement(8554..8853)
                     /locus_tag="VN968_21135"
     CDS             complement(8554..8853)
                     /locus_tag="VN968_21135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HXL31776.1"
                     /translation="MIYVVATSQVKPDQRDAFIKGARECIAATRKEKGCLSYESHTSI
                     NDPNLFVVVERWESREDLNAHGRAPHMKVWREYSAPLKVSPTVIEIISDGKVEKL"
     gene            complement(8881..9180)
                     /locus_tag="VN968_21140"
     CDS             complement(8881..9180)
                     /locus_tag="VN968_21140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HXL31777.1"
                     /translation="MIYVVATLTVKPETRAEFIAGAVACIHETRKEPGNIAYDLHESV
                     TDPSKMVFVEQWENAEALVPHRAAEHMKAFGRIAVRCMSAPPRIEVITPEKVDVR"
     gene            complement(9230..10942)
                     /gene="ureC"
                     /locus_tag="VN968_21145"
     CDS             complement(9230..10942)
                     /gene="ureC"
                     /locus_tag="VN968_21145"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HXL31778.1"
                     /translation="MSVKIKRSVYADMFGPTTGDRVRLADTDLIIEVEKDFTVYGEEV
                     KFGGGKVIRDGMGQSQVTNKQGAADTVITNALIVDHWGIVKADVAIKEGFISAIGKAG
                     NPDIQPGVTIVIGPGTDVIAGEGKILTAGGFDSHIHFICPQQIEHALMSGVTSMLGGG
                     TGPSHGTFATTCTPGPWHMARMIQSFDAFPVNLGISGKGNASRPAALVEMINAGACAL
                     KLHEDWGTTPAAINNCLSVADDHDIQVMIHSDTLNESGFVEDTVKAFKGRTIHAFHTE
                     GAGGGHAPDIIKIAGLKNVLPSSTNPTRPFTRNTIDEHLDMLMVCHHLDPSIAEDLAF
                     AESRIRKETIAAEDILHDLGALSMMSSDSQAMGRLGEVIIRTWQTADKMKKQRGALPE
                     DKGNNDNFRVKRYIAKYTINPAIAHGVSKLIGSVEKGKLADLVLWSPAFFGVKPECII
                     KGGSIVAAPMGDPNASIPTPQPVHYQPMFAAYGKSLTASSVVFSSKAAVASGLAKKLG
                     IDKKLYAVENTRGGISKKSMIHNGATPRIEVDPETYEVRADGELLTCAPAEVLPMAQR
                     YFMF"
     gene            complement(11019..11558)
                     /locus_tag="VN968_21150"
     CDS             complement(11019..11558)
                     /locus_tag="VN968_21150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXL31779.1"
                     /translation="MLAGLRLVSEAADLAARRHNGMQRKGRGNEPYINHLAEVANLLA
                     AATDGADAELVAAGWLHDTIEDTATTQEELVGIFGKRVSALVVEVTDDMTLPKSERRQ
                     KQIVDAPKKSVSAKLIKIADKVSNIRARILPQPNRDERDDLIDYVAWAEKVVAGCCGV
                     NAVLDRTFDETVKLARSTL"
     gene            complement(11563..11868)
                     /locus_tag="VN968_21155"
     CDS             complement(11563..11868)
                     /locus_tag="VN968_21155"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HXL31780.1"
                     /translation="MIPGELFIQDGEIELNAGRKTVTLSVANSGDRPIQVGSHYHFLE
                     TNPALKFDRRRARGMRLDIAAGTAVRFEPGQTRDVQLVALAGKRVVYGFRGDVMGKL"
     gene            complement(12056..12358)
                     /locus_tag="VN968_21160"
     CDS             complement(12056..12358)
                     /locus_tag="VN968_21160"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HXL31781.1"
                     /translation="MNLSPREKDKLLISMAAMVARRRLERGVKLNHPEAIAIISDFIV
                     EGARDGRTVAELMQAGAEVITRAQCMDGIAEMIHDIQVEATFPDGTKLVTVHEPIR"
     gene            complement(12399..13235)
                     /locus_tag="VN968_21165"
     CDS             complement(12399..13235)
                     /locus_tag="VN968_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HXL31782.1"
                     /translation="MRNDTTSAASAIFAANRARGSVTFDVHQVEGVTRRRQLHESGSL
                     RVRFPSPEAEGLSAVFVNTAGGVAGGDRFDIDIATGEGSHLAVTTAAAEKIYRAQGPA
                     AELNIALKLAAGSHLAWLPQETILFDRARVKRRIDIDLAEGASLLLCEIVVFGRAAMG
                     ERMRQGEFVDRWRMRRSGKLALAETVRLDGDIGEKLSRPAMAKGGVAIGTALIVPGDE
                     ALVERLRELSKSFGGEVGISCWNGFAMARFCAQDAARLRADMMAVLGRASGLALPRLW
                     LN"
     gene            complement(13394..14089)
                     /gene="urtE"
                     /locus_tag="VN968_21170"
     CDS             complement(13394..14089)
                     /gene="urtE"
                     /locus_tag="VN968_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HXL31783.1"
                     /translation="MLDVKDINLYYGAAQALRGVSISAEPGKVMCVLGRNGVGKTSLL
                     RAMVGQYPIASGSITFDGVDITGLKPYERARRGIGFVPQGREIFPLLTVEENLKTGFG
                     PLKRDDRNIPDDVFSLFPVLQTMLGRRGGDLSGGQQQQLAIGRALVMRPKLLLLDEPT
                     EGIQPSIIKDIGRAISYLRSLGNIAIVLVEQYLDFACELGDNFAVMDRGAVKFACDRS
                     TMDPGEISRQMAL"
     gene            complement(14209..14970)
                     /gene="urtD"
                     /locus_tag="VN968_21175"
     CDS             complement(14209..14970)
                     /gene="urtD"
                     /locus_tag="VN968_21175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HXL31784.1"
                     /translation="MNVMDTRATSALLYLDGVHVSFDGFHAINNLSLTLAPGEMRAVI
                     GPNGAGKTTMMDIITGKTKPDEGEVLFDGVTDLTRLDETRIAKLGIGRKFQKPTVFES
                     QTIEDNLLLALNVDHRVRGTLFWRETRDESERIERVLETIRLTDARDRLAGSLSHGQK
                     QWLEIGMLLAQDPKLLLVDEPVAGMTDVETHQTAELLKEINKDKTIMVVEHDMTFVRE
                     LGVKVTCLHEGTVLAEGTIDQVSSNERVIEVYLGR"
     gene            complement(14975..>15882)
                     /gene="urtC"
                     /locus_tag="VN968_21180"
     CDS             complement(14975..>15882)
                     /gene="urtC"
                     /locus_tag="VN968_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HXL31785.1"
                     /translation="MGMYLMRQIGSRGVYGNPILPDFMVFLNYPKLPWYWHGFDMFWF
                     AGLMILLVPGLLAFCFGWLAFRSRVTGVYLSIITQAMTYALLLAFFRNDFGFGGNNGL
                     TDFKDILGFNVQADGTRAALFLLSCLSLIIAFLICRAVVTSKLGKVLIAVRDAESRTR
                     FLGYRVESYKLFVFTLSACMAGVAGALYVPQVGIINPGEFAPGNSIEAVIWVAVGGRG
                     TLIGAALGAVVVNYAKTVFTSGPLAPYWLFMLGALFILVTLLLSRGIVGTFNAWWEPW
                     KAQRLTAAAESAAREDGVSEPNPAE"
BASE COUNT         3078 a         4889 c         4850 g         3065 t
ORIGIN      
        1 ggctccagcg gttgccaagc ggccgtcctg caggaagacg accgcattca ctgcgtcaga
       61 atgaaagcga agaacctgct ccgcggagtc ggtcgcaagc gaccatcgga tggcggaagt
      121 gtcgaaactg ccggaaatga cggtcttccc gtcggctgaa atggccagcg ctcgaaccgg
      181 tccgccatgg ccggaaagct gagcccaggc agcttcgaca tccagcgaca gcgccgcagc
      241 gattgcgcag agatacaggc gcaagttcat cgcttatcca tcgctgtcgt gccggcacgc
      301 gaagcgcgat cgtgcctcaa tccgaccaga cggtagagga catcgtggcg cgaggtcaaa
      361 ccagatccat cggaccatta ctgaggcttc accatgcttg agccttcggg tgcggcttgc
      421 atagggggcg ggcttcgtcc ttgaggactc tcggcgggag acgtgcggcc ggttgtcgaa
      481 ttgagcgaaa ttcccttcgt ttgattcgtc tgcgccccgg ccaatccgca gaccaaggcc
      541 aagcaaggca ttgtgtggcc gcgacgaacc gcaccggagt tttccgcctc gttgtcaatg
      601 caggttcaag cgcaaacgcg tgttcagttc catgttgcac tgcggccaga gcgcaggttt
      661 tcccacccgg tgctgaatgg agcgttggct gcgcgctttt atcatcttga gccgtaaccg
      721 ccagacagcc tatcgtgcac ttccttcgta agaaggaggg cgagatgcat atacgatcca
      781 agacgtatgg gcacttcctt tcaatttgcc ggtcgctccg cgatctaatg tcgccgcacg
      841 atcagagtgt cccaacagag acctcgccct cactcgtttt cgccacgacc gtgttggcgc
      901 ttcttttgac gatcctggag atagatcttc acggagctga actgcatcta gtggggctta
      961 tgcatgatac ggacgccctt aatcctattt ttatgagccc atagtttctt gcatagtcga
     1021 actcttgctg cgtgcccgac cggagccatg gccgaggtct attcgtgtga aattaaggaa
     1081 agggtgcggg taaatgacga gggacttgtt cggcccgcgc cgagcgcacc tttaaacgcc
     1141 aagtatcgat cgacaaagaa accggcaatg ctgtcggcga tgcgctgtcg gctgcaaatc
     1201 gttgggaagt tgttcaaaag agttcgcaag aaaacgcctt tgtgactcct gagggtgacc
     1261 ccacattgga gatcacggct ggcggaccta catcggcatt cgttaaggca gccggcgagg
     1321 cgacaaaaac gggttcagga tcgtcaaaac cggatatcct gagtcgcgat gcgcgatacg
     1381 tcgcgataga tcggactgtc ccatttcttc gctatgggta tccaatctcc gcacgcacat
     1441 gtcatcgtgc ttccctttcg ttttgccaag tcgcagctct acgtcgaacg cagctgtgtc
     1501 gacgggccca aaaaaagaaa agacaccgac tccggcaagc gatgtgttgc gcgatgcgaa
     1561 ggatgctccg ttagaaggcc tcggttggat ccacggcttc attccgtcgt cgcccttgct
     1621 cttcaagcgt gcagtgcgtc gtcctgagtt gcgcaagatc gcgcggttgg ccaacacgac
     1681 cgccttcgaa catgatgatc tcacccgggc gctcgcgctc tgaaagctgc cggaggcttt
     1741 catcttcaag gcccacggca aattgattgg cgaacagttg gagcgtccgc acatcgcaaa
     1801 aaatccgacg cgccgttttg cgcgccggat caacttaatc gatggcctgc ttcagcacgc
     1861 cgccattcgc ttcgcgccta cgccaccctc ggcgcgcggt tgcgcgagtt gcgctggaag
     1921 aacagcgcct ggctggcgac ggcggatacc atcgcgggct ggaacggttt tgagatcagg
     1981 aacgccggtt ccgggcgttc gccggtcagg aagcgttccg gataggcggt gatgaacacc
     2041 accggcactt cgaagatccg caacagctcg ttgacggcat ccaggccgga gctgccgtcg
     2101 gcgagttgga tgtcggccag gatcaggccc ggcttcctgt tcttggccag cgccaccgca
     2161 tcggagtggg tgcgggcgac gccgatcacg ttgtgaccga ggttcttcac caggctctcc
     2221 agatccatcg cgatgaacgt ctcatcctcg atgatcagca cgtcggtggc gatctccgcc
     2281 gccatttcac ggccggcggt atcggcgagt tgccgggtct cggcgacctc gatgccgagg
     2341 acgaacgcca cttcctcttc cgaaaaacct tccagcgaca gcagcaggaa ggcctggcgc
     2401 ggcaacgggg tgatattgga aagccggcgc tccgaaggca tcggcagcgt cgtcacgtca
     2461 gcatcggtat cgtcgttgag cgaaaccgaa ttccagattt gggtaaatag ccggaacaaa
     2521 ccggcgcggg gtccgtgcgc ctcgtcgagc agagagggat cctgaagcag ggcttcaagc
     2581 atggcgccga cataggcgtc gcccgacgcc tggtttcccg tcaacgcacg ggcatagcgc
     2641 cgcaacaacg gcaaatgttc ggcaacgagc tgtgatcggg acatccccac tccatatttc
     2701 ctgagtttca gccttggacc agttcccgaa tcggttcttg atcagtttct gggccggttc
     2761 ttacgtcagt catttgggtc aaaccatcaa tgtaggggga cccggttccc ctgatggctg
     2821 actacgcacg aacacgagaa aagttcccgt gatccggaac tttccggcgg actttgcatt
     2881 agccttctat agagaggtct caacgatttt ggaaatcaag cataattatc gaagtttcag
     2941 ggacttaact ctcggggaag cgtggaaaag ctcatgaaag agataaagaa gccagccaag
     3001 ccgggcggcc taaatgctga gattcagtcg agaatcggcc accagctgcg tgccatgtac
     3061 gacgatgtgg tgcggcaagg ggttcccgat cgtttcgcgg aacttatccg aaagcttgat
     3121 acgcaggaag ccaatcccca attgggaact gcgggcgaaa aaaccgaggg gagcgactaa
     3181 tgcctctcac agacgcactc cgcgacgaca tcctggcgtc agtaccgagc ctgcgcgcgt
     3241 tcgccatctc gctcagcggc aatggcgacc gggccgacga tctcgtgcag gaaaccctgc
     3301 tgcgcgcgct tgccaacatc gactcgtttc agcccggctc caacctgccg gcgtggctgt
     3361 tcacgatcct gcgcaacctg ttccgctccg actatcgcaa gcggcgacgg gaggtggagg
     3421 acgccgatgg cagctatgcc aagacgctga aaacccagcc ggcgcagaac gcgcatctcg
     3481 agttcgagga gtttcgcgcg gcactggaaa aacttccgca ggaccagcgc gaagcgctga
     3541 tcctggtcgg cgcctccggc ttctcctatg aagatgccgc ggcgatctgc ggctgcgcgg
     3601 tcggcaccat caagagccgt gtcaaccggg cgcgatcgaa gctctcggcc ctgctctatg
     3661 tcgacggcac cgacgatttc ggccccgatg agacggttcg cgccgtgatc ggcggcagcg
     3721 gcggataagc cgttgtcatt ccccgatgtt aaatttcatg tcggggccaa gcgatttgaa
     3781 cccgggcctg cccgggtcag acaaacctat cggcccaaga ggactaacgg cgaggactaa
     3841 cggcaaaaat caacggccga gggaatgatt gactaggtcg gctcggcaaa ggccgacctg
     3901 gatgtagcag acccggaatt cagagatgat cggcgcgatt gtaggttggc gctgacgagt
     3961 accctggagt aaccagaatg gttgttgaca gacaggtgag gctgaacgcg cctcaatgcg
     4021 agccgtttca atatgagccc tatcggcacg aaaagcggcc acagatcgtg gccgcttttt
     4081 tgctgcatcg aattctagtg caaaccgccg aacgcgtaga tcaggaccag aatgggaagc
     4141 gggacaccga tcagccacaa caacaaataa cgtcccatga aagcgcctct ggttacgggt
     4201 cagttaatcc gtctcaattc ccgtaagccg cggtttgttc cggagcgtgc gatttggccc
     4261 cattcgatct tggattgatg cgccgtcatt ccggggcgcg tcaacgggtc ggcgctttgc
     4321 gccgcccgat gacaggctcc gcgcgagccc ggaatccata accaccgccg ggagcataga
     4381 ttccgggcct gcgcatccaa gtcggctgtt gccgacttgg acattgatat tgccgaactc
     4441 gggtaaaccc gaggtcggtg gcgcgtccac gatgtgcaat tgcacatcgg ggaatgacga
     4501 cggttgaggt ccatccaaga caaatcaagc taccgccccg gcctcggcgg cgccgacaat
     4561 tgttcggtcc ggtctcgctc cgtcaggtcg acgacggtct gcatcggagc ggtgagttcg
     4621 tagacgtaac tcacttcggt aaaatatcgc ttcgatacgc cgccgagcgc ttccggtgcg
     4681 acgcgatcga gcaggatgac gccgaaatcg ctgtcttcac ggcgggtcgc agcactggtg
     4741 ttgaattctt cccagcgaaa atggaacgtg gtgtcgggct cctctccggt cacttcccaa
     4801 tgcgcgacga tgtgggggtg cttgccgacc agcgagagcc cctcgtcgga atgcgatgcc
     4861 agttcgaaga acagcagcga aaggctctgg gcggcgcgcg cgctcacggc gacatcggga
     4921 ccgctgaccg cgatgcgctc ggcgtgggga atggcgcgcg cctcgaacag ccctttcagc
     4981 ttcacaccct gccactggct ttcgctgagc agcgagacca cgttcgacat cgcatggatc
     5041 cggccgatca ggaggtcacg ggcgacatcg atgtcggaac cgtggcgcag cgtgcgcgtc
     5101 acgatcgact ggatcaccgc caggatgttc ttgacccggt gattgagttc gtcgatgacc
     5161 gcggtcaagc ggcgctcgaa gccaattcga acctggattt cgcggctgag ccgcaaatta
     5221 ttgtaggcga catagccgaa aagcccgcag acgatgccgg tcagcgcaag gccgatggcg
     5281 gcgacgatgg cggcggtctg ctgcgcgcgc ttcaacgagt tggtcttggc gtaatagacg
     5341 agcccccagt cgcgacctcc gaacgcgacc gttcgcgtcg ccgacggcgt gggcgcctcc
     5401 ggcgctaccg tccgcgacgt cacgatgccc cgatcatctg aaaccagctc gccggcggcg
     5461 tggcgcgggt ctttcagcac gaccgagaac agcgacatct cgtcattcgc cagcatcagc
     5521 ggcgccagtt cataggaaaa ggtcacgaag cctgctggcg aagcatcggt ctcctgcaga
     5581 accggcgtgg caagaacgag gccgatctgc ccatccgggc gcaacagcgg tgcgggatcg
     5641 gacgctaccg gctttccgtc ggccatcgcc tgcgccagcg tcggtcccag gaccgggtcc
     5701 cgatcatagg aacggccgag gaaacgcagt gtctcggcat cgcgtggctc gacgtccatc
     5761 aggacgtcga ttggcttatc gagcgaccgc gtattcaaag gctgatcatc gaagccgcgg
     5821 atcgcaggat tcgaaaatcc agcggtcgcc agttccttgc gcgccgcatc cagttcgttc
     5881 ggcttcagcc gcgcgatcca gcttgcgaca atgaaatcgg tcttgaacgc gtagatcgag
     5941 gaccgcagcg gctgcagcat attggccttg accaccgaag gcgcacggaa cagtccggat
     6001 gcgacacgcg cgagcagttc acgctcggtg agccggtcct gaaccaggct ggcgtgaacg
     6061 tcgatggcgc gcgccagcgc gatgccgtcg accgtcagtt cctgatcgtg aacgcgataa
     6121 gccgcgagac cggagagcag tgctccgatc agcgcgataa acccgatgat gaaacccagc
     6181 cgaaccactc gcttactcag aaaatgagga gctggcgaaa aaatatctgc cgtccgccgg
     6241 ggcggcaaca agccgaagca ggatgaaagc caaccgtcag ggataatgaa gcagcgatca
     6301 ttggtacgca actaaggtcg gcgatgaaga ttaatatgac gcctcgtaag attaatatcg
     6361 ttgccgcgcg tgtcgcggtg gcgaagcccg gagaatgtta atcacagcga gccgatttag
     6421 ttccgacgac gcgcggaagt ttttaacctt gcttgtttaa ccttgcttgc atccgggact
     6481 cgcggccaca agcgttaacg aatggcgtca gccgaccttg gcttgcgccg gcgagctgag
     6541 accgcctttc gcctcgatga agccgataag gcggtcgaga ccttcgctct tcctcagatt
     6601 ggtcatgacg aagggccgtt cgccgcgcat ccgtctcgca tcggcagcca tcttttccag
     6661 cgacgcgccg acatgcggcg cgaggtcgat cttgttgatg acgaggagat ccgacctcgt
     6721 gatcccgggg ccgcccttgg aagggatctt gtcgcccgcc gcgacgtcga tgacgtaaat
     6781 cgtgagatcg gcgagctcgg gcgagaacgt ggcggcgaga ttgtcgccgc cggattcgat
     6841 cagcaccatg tcgagatcgg gaaatttcgc gcgcatgtcg gcgaccgcgg cgagattcat
     6901 cgaggcgtcc tcgcgtatcg cggtgtgcgg acagccgccg gtctcgacgc cggcgatgcg
     6961 atccggcgtc agcgatcccg agcgcaccag aaactccgcg tcccatttgg tgtagatgtc
     7021 gttggtgatc gcggcgatgt cgtaacgctc ccgcatggcc ttgcagagca gatccatcag
     7081 cgcggttttt cccgatccga cgggaccacc gatgccgatg cgaagcgggc cgttcgtctt
     7141 agacatgttt gatgctcgtt ccgctacgat gtcgtcccgg ctttcgccgg gacgacgcac
     7201 tgaaatgcat ggcgccgcgc tgatattatc acgagcgaaa caatctcgta tactgcgtct
     7261 cgtgccgcat gccggcaaga tcggcgcgaa aggtcgcgct gccgagatcg tcgagcgatg
     7321 catccagcgc gcgttgtcca gttgcgacga cgatcggctc gagcaaggcc agcacgcgct
     7381 ggctgtcggt ctgtcccagc ggaatgagcc gcgcgccggc ggaaatccag ttcgacgtca
     7441 gcgcgtgcag gaacgcgtgc atcgtgggcg cgaggggtat cgcatgcgcg gccgagacca
     7501 gacccaccgc gacgggataa acgatggcgc cgtcgcaacg ggaaatcatc gcgtccagcc
     7561 cgtcgcaatt ccaggcggag cgcgcaatct cgatgaacgc acggccctgc gtcgttgtct
     7621 cgagctggcg ctcgcgcgaa gccacgaagg cagcggcaag ctccgcggtt tcccgcaagc
     7681 cggcctcgtc atgcgacgat gcggcgcggt gggcgtggca aagcaacacg ccatcgcaaa
     7741 atcccgtgcc gtccgccagc atcgaggcaa gccaatcgcg cagggatgcc gcgtcgacga
     7801 tatcgccggc ctccaccgcc cactcgatgc cgctggaata ggagaacgcg ccgaccggaa
     7861 aggccggcga cagccaggtc atcagccggt acaacgccgc ggcctcacct tgcttcattc
     7921 cgctgcgatc attggtcggc ggtgccttac ttgtggtcat gagcatggga atggccatga
     7981 tgatgatcgt gatcgcaatg ctcgtcatgg tgatggtgat caccgtggtg atgatgttcg
     8041 ccatgctcat gcgatggacc atcatgcgcc gcgtgaccat gcggatcgct ttcggccgcg
     8101 tgcgcatggc cgccgccggc ataggcgccg ccttcgggat cgaacggcgc ctcgatctcg
     8161 atgacgcggg caccgagccc cttcaccatc gcctcgatca catgatcgcg ccggatccgc
     8221 aggcttttcg gcatgatctg tgtcggcaga tggcgattgc cgagatgcca ggcgacccga
     8281 accaggtgct gcgggtcttg cccacgaatt tcgagcagcg actccggcgc cgccacgact
     8341 tcaatcaacc tgttatcgtc gagcaccagc gcatcgccgc cgcgcagcgc aaccgcgttt
     8401 tccagatcga gcaggaactc caatccccgc gtgccggtca tcgccatccg ccgccggtga
     8461 cggtcgtcga aatcgagcac gacggtatcg gccggcgcct ccgtccagcg atgtgcgccg
     8521 cggaccctgg tcgcgcggat catgaaaaat tgctcacagc ttctcgacct tgccgtcgct
     8581 gatgatctcg atcacggtcg gcgacacttt tagcggcgcg gaatattcgc gccagacctt
     8641 catatgcggc gcccggccat gggcattgag gtcctcgcgg ctttcccagc gctcgaccac
     8701 cacgaacaaa ttcgggtcgt tgatgctggt gtgactctca taggagagac aacctttttc
     8761 cttgcgggtt gccgcgatgc attccctggc gcccttgatg aaggcatccc gctggtccgg
     8821 ttttacctga gaggtggcga cgacgtagat catggatttg gtctcccgat tttttcttgc
     8881 ttatcggaca tcgacttttt caggcgtaat cacctcgatc cggggcggtg ccgacatgca
     8941 tcttaccgcg atacggccaa acgccttcat gtgctcggcg gcgcgatgcg gcaccagcgc
     9001 ctcggcgttt tcccattgct cgacgaatac cattttggag ggatcggtga cgctctcatg
     9061 cagatcatag gcgatattgc cgggctcttt gcgggtttca tggatgcagg caacagctcc
     9121 ggcaatgaac tcggcgcggg tttcaggctt cacggtcagg gtagcaacga cgtagatcac
     9181 gataatcctc ccggtctttt ggtctggctt gagcgtgccg ggagtgagct tagaacatga
     9241 aatagcgctg cgccatgggc agcacctcgg ccggcgcgca ggtcagcaat tcgccgtcgg
     9301 cgcggacctc ataggtctcg ggatcgacct cgatcctggg cgtggcgccg ttgtggatca
     9361 tgctcttttt cgagatgccg ccacgggtat tttcgaccgc gtagagtttc ttgtcgatgc
     9421 cgagcttctt ggcaagcccc gaagcgaccg ccgccttgga ggaaaacacc accgaggagg
     9481 cggtgagaga tttgccatag gcggcgaaca tcggctggta gtgcaccggc tgcggcgtcg
     9541 gaatcgaggc gttgggatcg cccatgggag cagcaacaat cgagccgccc ttgatgatgc
     9601 attcgggctt gacgccgaag aaggccggcg accacagcac gagatcggcg agcttgccct
     9661 tttccaccga gccgatcagc ttcgagacgc catgggcgat cgcggggttg atggtgtatt
     9721 tcgcgatata gcgcttgacc cggaaattat cgttattgcc cttgtcttcc ggcagcgcgc
     9781 cgcgctgctt cttcatcttg tcggcggtct gccatgtgcg gatgatgact tcgccgagcc
     9841 ggcccatggc ctgcgaatcc gacgacatca tcgacagcgc gccgagatca tgcaggatgt
     9901 cttccgctgc gatggtttcc ttgcggatac ggctttcggc gaacgcaagg tcctcggcga
     9961 tcgagggatc gagatggtgg cagaccatca gcatgtcgag atgctcgtcg atggtgttgc
    10021 gggtgaacgg ccgcgtcgga ttggtcgagg acggcagcac gtttttcaaa cccgcgatct
    10081 tgatgatgtc aggcgcgtgg ccgccgccgg caccttcggt atggaaggcg tgaatggtgc
    10141 ggcccttgaa ggcttttacc gtgtcttcga caaaacctga ctcgttcagc gtgtccgagt
    10201 ggatcattac ctggatgtcg tgatcgtcgg ccaccgaaag gcagttgttg atcgcggccg
    10261 gcgtggtgcc ccagtcctcg tgcaacttca gcgcgcaggc gccggcgttg atcatctcca
    10321 ccagcgccgc cgggcgcgac gcgttaccct tgccggaaat gccgagattg accgggaacg
    10381 cgtcgaacga ctggatcatc cgcgccatat gccagggacc gggcgtgcag gtcgtcgcaa
    10441 aggtgccgtg cgacggcccg gtgccgcccc ccagcatcga ggtgacgccg gacatcagcg
    10501 cgtgttcgat ctgctgcggg cagatgaaat ggatgtggct gtcgaacccg cccgcggtca
    10561 ggatcttgcc ctcgcccgcg atcacgtcgg tgccgggacc gatgacgatg gtcacgccgg
    10621 gctggatgtc gggatttccg gccttgccga tggcgctgat aaaaccttcc ttgatggcga
    10681 cgtcggcctt cacgatcccc cagtgatcga cgatcagcgc attggtgatg acggtatcgg
    10741 ccgcaccctg cttgttggtg acctgggact gacccatgcc gtcgcggatc accttgccgc
    10801 cgccgaattt cacttcctcg ccgtagacgg taaaatcctt ttcgacctcg atgatgaggt
    10861 cggtgtcggc gagccgcacc cgatcgccgg tggtcggccc gaacatgtcg gcatagacgg
    10921 aacgttttat ttttacggac atgacttggc tccatcggca gacgtgtccc ctgccccctt
    10981 gcgggggagg gttaggaagt ggggtgaaca cgccatgctc aaagcgtgct ccttgccagc
    11041 ttcacggttt cgtcgaaggt gcggtcgagc accgcgttga caccgcagca tcccgcaacg
    11101 accttctcgg cccaagcgac gtagtcgatc aaatcatcgc gctcatcccg gttcggctgc
    11161 ggcaagatac gcgcgcgaat attgctgacc ttatcggcaa tcttgatcag ttttgcgctc
    11221 accgatttct tcggcgcatc gacgatctgt ttttggcggc gctcgctctt cggtagcgtc
    11281 atatcgtcgg tcacttcgac aacgagcgct gagacgcgtt tcccaaaaat tccgaccagt
    11341 tcctcttgcg tcgttgcggt gtcctcaatg gtgtcgtgca gccagccggc ggccacaagt
    11401 tcggcgtccg ccccatcggt cgcggctgca agcaggttag cgacctcggc gagatgattg
    11461 atgtaaggct cattgccgcg ccccttccgc tgcatgccgt tgtgacggcg cgccgcgaga
    11521 tcggcagctt cggaaaccag gcgtaagccc gcgagcatgg cttcacagct ttcccatcac
    11581 gtcgccacga aagccgtaga cgacgcgctt gccggccagc gctacgagct gcacgtcgcg
    11641 ggtttggccc ggctcgaagc gcaccgcggt gccggcggcg atatcgagcc gcatgccgcg
    11701 ggcgcgtctg cggtcgaatt tcagcgcggg attggtctcg aggaaatgat aatgcgagcc
    11761 gacctgaatc ggacggtcgc cggagttggc gactgatagc gtcaccgtct tgcggccggc
    11821 gttgagttcg atctcgccgt cctggatgaa tagttcgccg gggatcatga ttgctgcctc
    11881 gcaaaattct gctgcgtcat ggccgggctt gtcccggcca tctacgtcct actttctgat
    11941 tcaagaaaag gcgtggatgc ccggcatcgc agacaagtgt acgcagtctg cgcaaagcag
    12001 actgctatgc ccgggcatga aattgaaggc ttcccgccgc cgcgaaatga cgatctcatc
    12061 ttatcggctc atgcacggtc acaagcttgg tgccgtcggg aaacgtcgct tcgacctgga
    12121 tgtcgtgaat catctcggcg atcccatcca tgcactgcgc acgggtgatg acctctgcgc
    12181 cggcctgcat cagttcggct acggtgcggc cgtcgcgcgc gccctcgacg atgaaatcgg
    12241 aaatgatcgc gatagcttcc ggatggttga gcttgacgcc gcgctccagc cgccgtcgcg
    12301 ccaccatcgc cgccatcgag atcagaagct tgtccttttc gcggggagac agattcatgc
    12361 gagcactcta atcattttga aacatctaat tgagccacct aattgagcca gagccgcggc
    12421 agcgccaaac cggaggcgcg gccgagcacc gccatcatgt cggcgcgaag cctggccgca
    12481 tcttgggcac agaagcgcgc cattgcaaac ccattccagc aggagatccc gacctcgccg
    12541 ccgaacgact ttgacagttc gcgaagccgc tccaccagcg cctcatcgcc gggtacgatc
    12601 agcgcggtgc cgatggccac gccgcccttc gccatcgcag gcctggaaag cttctcgccg
    12661 atgtcgccgt cgagccggac ggtttccgcg agcgcgagct tgccgctgcg ccgcattcgc
    12721 cagcgatcga cgaattcgcc ttgccgcatt cgttccccca tcgcggcgcg gccgaatacc
    12781 acgatctcgc agagcagcag cgaggcgccc tccgccaggt cgatatcgat ccggcgcttt
    12841 acccgcgccc ggtcgaacag aatggtctcc tgcggcagcc aggcaagatg cgaacccgcg
    12901 gcaagcttca gggcgatgtt gagctctgcc gcgggcccct gcgcccggta gattttttcc
    12961 gccgccgccg tcgtcaccgc cagatgcgat ccctcgccgg tcgcgatgtc gatgtcgaag
    13021 cggtcgccgc cggcgacgcc gcccgcggta ttgacgaaaa ccgccgagag accttccgcc
    13081 tccggtgacg gaaatcgaac gcgcaacgag ccggattcat gcagctggcg gcggcgggtg
    13141 acaccctcga cctgatgcac gtcgaacgtc accgagcccc gggcgcggtt ggccgcgaaa
    13201 atcgccgaag ccgcactcgt ggtatcattc cgcatccgcc cccaaacctt ccccaaaagg
    13261 gcgcgcgacc gccgccggtc gcgttacacg ttgcaagatc atgatccgat tggatcgaat
    13321 cggatcatga tctaatcctt ttgtttgagc atgatctttt cggaaaaccg gttcccactt
    13381 ttccggatca tactcagagc gccatctggc ggctgatttc accgggatcc atcgtacttc
    13441 gatcgcaggc aaatttcacg gcgccccggt ccatgaccgc gaaattgtcg ccgagctcgc
    13501 aggcaaagtc gagatattgt tcgaccagca cgatcgcgat gttgccaagg ctgcgcaaat
    13561 atgagatcgc ccgcccgatg tccttgatga tcgacggctg aatgccctcg gtcggctcgt
    13621 cgagcagcag cagtttcggc cgcatcacca gcgcgcggcc gatcgcgagc tgctgttgct
    13681 ggccgcccga cagatcgccg ccgcggcggc cgagcatcgt ctgcagcacc gggaacagcg
    13741 agaacacgtc atcgggaata ttgcggtcgt cgcgcttgag cggcccaaag ccggtcttga
    13801 gattttcctc caccgtcagc agcggaaaga tctcgcggcc ctgcggcacg aagccgatgc
    13861 cgcgccgcgc ccgctcatag ggtttcaagc ccgtgatatc gacgccgtcg aaggtgatcg
    13921 agcctgaggc gatcgggtat tggccgacca tggcgcgcag caggctggtc ttgccgacgc
    13981 cgttgcgccc caacacgcac atcaccttgc ccggctcagc cgagatcgag acgccgcgca
    14041 gcgcctgcgc cgcgccgtag tacaggttga tgtccttgac gtcgagcatc agcgacctcc
    14101 ggtcgcgcga atggcgaata gcgaatagcg agtggttgaa atcgtcgcac ccatcgttcc
    14161 cagaccctca ttctattcgc tattcgccac tcactattcg ctactcgcct accgccccag
    14221 atacacttcg atcacccgct cattcgacga aacctgatcg atggttccct ccgccagcac
    14281 cgtgccttcg tgcaggcagg tgaccttgac gccgagctcg cgcacaaaag tcatgtcatg
    14341 ttcgaccacc atgatcgtct tgtccttgtt gatttccttg agcagctcgg cggtctgatg
    14401 ggtttcgaca tcggtcatcc cggcaaccgg ctcgtcgacc aatagtaatt tcggatcctg
    14461 cgccaatagc atgccgatct cgagccactg cttctggccg tgcgacaggc ttccggcgag
    14521 gcggtcgcgc gcgtcggtca ggcgaatggt ttccagcacg cgctcgatgc gctccgactc
    14581 gtccctggtt tcccgccaga acagcgtgcc gcgcacgcgg tgatcgacgt tgagcgccag
    14641 cagcagattg tcttcgatgg tctggctttc gaataccgtc ggtttctgga atttccggcc
    14701 gatcccgagc ttggcgatcc tggtttcgtc gagccgggtc aggtcggtga cgccgtcgaa
    14761 cagcacctcg ccttcatccg gcttggtctt gccggtgatg atgtccatca tcgtggtctt
    14821 gccggcgccg ttcgggccga tgacggcgcg catctcgccg ggcgcgagcg tcagcgacag
    14881 attgtttatg gcgtgaaagc cgtcgaacga gacgtgcacg ccgtcgagat agagcagcgc
    14941 cgaggtagcc ctggtgtcca tgacgttcat gcgctcactc cgccgggttc ggttcgctga
    15001 cgccgtcttc gcgcgccgca ctctcggcgg ccgccgtcag gcgttgggcc ttccacggct
    15061 cccaccacgc attgaaggtg ccgacaattc ctctcgacaa cagcagcgtc accaggatga
    15121 acagcgcgcc cagcatgaac agccagtacg gcgccagcgg gccggacgtg aacacggtct
    15181 ttgcatagtt gacgacgacg gcgcccagcg ccgcgccgat cagcgtgccg cggccgccga
    15241 ccgcgaccca gatcaccgct tcgatggaat ttcccggcgc gaattccccg ggattgatga
    15301 tgccgacctg cggcacatag agcgcgccgg cgacgccggc catgcaggcc gacagcgtga
    15361 acacgaacag cttgtaggat tcgacgcggt agccgagaaa ccgggtgcgg gactcggcat
    15421 cgcgcaccgc gatcaggacc ttgccaagct tcgaggtgac gaccgcgcga cagatcagga
    15481 aggcgatgat cagcgaaaga cagctcaaga ggaacagcgc cgcgcgggtg ccgtcggcct
    15541 gcacgttgaa gccgaggatg tccttgaaat cggtcaggcc gttgttgccg ccgaagccga
    15601 aatcgttgcg gaagaaggcc agcagcagcg cgtaggtcat cgcctgcgtg atgatcgaga
    15661 gatagacgcc ggtgacgcgg gagcggaacg ccagccagcc gaagcagaag gcgagcaggc
    15721 ccggcaccag gagaatcatc aggcctgcga accagaacat gtcgaacccg tgccaatacc
    15781 agggcagttt cggatagttc aggaacacca tgaagtcggg caggatcggg ttgccataga
    15841 cgccgcgact gccgatctgg cgcatcagat acatgcccat gg
//
LOCUS       DATNOI010000523         3885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1107215,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000523 DATNOI010000000
VERSION     DATNOI010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107215"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2884
                     /locus_tag="VN968_21185"
     CDS             <1..2884
                     /locus_tag="VN968_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXL31786.1"
                     /translation="RVVAMVPEQGQRPVYASAGWEIDLARRELRSSGVPVPLGGRAFE
                     IVAELVQSGGELVSKTDLAERVWPRTFVEEGALRVHIAAIRKAFGPDRDMLSTAVGRG
                     YRLLGAWQVRQDSTPAAQAAVEPMQARVDPVLTNLPVARFDLIGRAAAIPYLRDLLSA
                     YRVVTLTGPGGIGKTTLALEVARSVFPDLQGGSLLVELASLSNPDLVPSALASVLGLK
                     LDGEEISAESVARAIGAREFLLVLDNCEHVVDAVANLTEAIVSRCPRTTVLATSREVL
                     RVHGEHVFRVPPLTVPPGTRVEPNDALGHAAVELFIARAGALGSDFKRNEENFSAIAS
                     ICRRLDGIPLAIEFAAAHAVMMGPPEIAALLDDRFKFLTTGRRTALPRHQTLRATLDW
                     SYDLLPETEARVLRHLAVFAGDFLLDAAIAVAGAASSVTDHLANLVAKSLVVADVRGQ
                     TPHYRLLDTTRVYALEKLRSGGEYREAARRHAEYYRDFFAQAEAESESKPQAEWLAIY
                     GRHIDNVRASLNWAFTPDGDPQIGVALTATAVPLWVQLSLLGECRERAQMALASLGNG
                     AAATARLRMQLSAALGWSLMYGVGRAREAGPAWATTLELADQLDDREYRLRALWGLCI
                     DQFNNGEIRKALEFAHRFVDGAANSTDPIDSMMADRLLATALHYLGDQKSARHHIERV
                     LVHLADLTLKPRIVRFRFDLRLSTHYFQARILWLQGFADQALRAVERNIEEGRASGHA
                     LTFCSVLGQAACPIAFLAGDLDAAERYCAALLDHTDRHPIRLWNLWARCFKGIVMIKR
                     GDIAAGLEVLRSELGRAGEARFLPRFLLPLGELAACLGEAGEVAQGLATVDEALLRCE
                     ARDERWYVAELLRIKGELLLHETEHRSIPAAEQCFGEALELARRQGALFWELRSALSL
                     ARLRIGQDRRADARAVLAPAYEKFAEGFETADLRNAGAMLEQLSAS"
     gene            complement(3036..3335)
                     /locus_tag="VN968_21190"
     CDS             complement(3036..3335)
                     /locus_tag="VN968_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31787.1"
                     /translation="MAEADLDIVIRQLAKQQNKGLMTAVKRRRDRYVSLAAKAKDKDT
                     KEKYRQTAKNIMLQGTAAAKRLQISADNAADSYARSMRNAAEAQPAKKAVKKTKT"
BASE COUNT          663 a         1209 c         1234 g          779 t
ORIGIN      
        1 tagggtggtc gcgatggtgc ccgagcaggg ccaacgaccc gtatatgcct ccgccgggtg
       61 ggagatcgat cttgcccggc gcgaattgcg ttcgtcgggg gtgccggtgc ccctcggcgg
      121 ccgcgcattc gaaatcgtcg cggagctggt acagtcgggc ggcgagctcg tcagcaagac
      181 cgacctggcg gagcgcgtct ggccacgcac cttcgtcgaa gagggcgcac ttcgggttca
      241 tatcgcggca attcgcaagg cattcggccc tgatcgcgac atgctctcga cagcggtcgg
      301 tcgcggctat cgcctgctcg gggcctggca ggtccggcag gacagcacac ccgcggcaca
      361 ggccgcggtc gagccgatgc aggcgcgcgt cgatcccgtt ttgacgaatc ttccggtggc
      421 gaggttcgac ctgatcggcc gggccgcggc cattccttac ctgcgggatc tgctgtcggc
      481 ctatcgggtg gtcacgctga ccggtcccgg tgggatcggc aaaacgacac tggcgctcga
      541 ggtcgcccga tccgtattcc cggacctgca gggcggcagc ctgctggtcg agttggcctc
      601 gctttccaac ccggacctgg ttccctcggc gctcgccagc gttctcggtc tcaagctgga
      661 cggcgaggag atttccgccg agtcggtcgc gcgtgcgatc ggggccagag aattcctgct
      721 cgttctggat aattgcgagc acgtggtcga cgctgtcgcc aacctgaccg aagcgatcgt
      781 gagccgttgt ccgcgcacca ccgttctcgc aaccagccgc gaagtactgc gcgtccatgg
      841 tgagcatgtg tttcgcgtcc ccccgctcac cgtgccacct ggaacccggg tcgaacccaa
      901 cgacgccctt ggacatgcgg ccgtggagct gttcatcgcc agggccgggg cgttaggctc
      961 tgatttcaaa cgcaacgaag aaaacttcag cgcgattgcc tcgatctgcc ggcggctgga
     1021 cggcatcccg cttgccatcg aattcgccgc ggcccacgcc gtcatgatgg gacctccgga
     1081 gatcgccgcc ctgctggatg accggttcaa atttctgacc accggccggc gaacggcgct
     1141 gccgcggcac cagacgctgc gcgcgacgct cgattggagc tatgacctgt tgccggagac
     1201 cgaggcgcgg gtgttgcgcc accttgccgt gtttgccgga gatttcctgc tcgacgcggc
     1261 gattgccgtg gcaggagccg catcgtccgt taccgatcac ctggccaatc tggtggcgaa
     1321 gtcgctggtg gtcgccgatg tccgtggcca gaccccgcat taccgtctgc tggacaccac
     1381 ccgcgtctat gcgttggaga agctgcgcag cggcggcgag tatcgggaag ctgcacgccg
     1441 tcatgccgaa tattatcgcg acttctttgc gcaggccgaa gccgagagcg agtcgaagcc
     1501 gcaagccgag tggctggcga tctacggccg gcacatcgac aacgtccgcg ccagcctgaa
     1561 ttgggcattt acaccggacg gagatccgca gatcggcgtg gcgttgaccg ccaccgcggt
     1621 gccattatgg gtgcagctgt cgctgttggg cgagtgccgc gagcgcgccc agatggcgtt
     1681 ggcgagcctc ggcaatggcg cagccgccac ggcacggctg cgcatgcagc tttccgcggc
     1741 acttggctgg tcgctgatgt acggtgtcgg ccgtgcccga gaggctggcc cggcatgggc
     1801 gacgacgctg gaactcgccg accagctcga tgacagggag taccggctgc gcgcgctctg
     1861 gggtctgtgc atcgatcaat tcaacaacgg cgaaattcgc aaagcgctcg aattcgccca
     1921 tcgtttcgtc gacggggcgg caaactccac cgacccgatc gactcgatga tggccgatcg
     1981 ccttctcgcc accgcgctgc attacctcgg cgaccagaaa agcgcgcgtc atcatatcga
     2041 gcgcgtgctg gtgcatctgg cggacttgac gttgaaaccg cggatcgtcc gcttccggtt
     2101 cgatctgcgg ctgtccacgc actacttcca ggcgcgtatc ctttggctgc aaggcttcgc
     2161 cgatcaggcg ctgcgcgcgg ttgaacgcaa tatcgaggaa gggcgcgcca gcggacacgc
     2221 cctgacgttt tgcagcgtgc tgggacaggc ggcgtgcccg atcgctttcc tggccggcga
     2281 tctcgacgcc gccgagcgct attgcgccgc gctgctcgac cataccgacc gccacccgat
     2341 acgtctctgg aatctgtggg cgcgctgttt caagggcatc gtgatgatca agcgtggcga
     2401 tatcgccgcc gggctggagg ttctgcgaag cgaactcgga cgcgccggag aggccaggtt
     2461 cctgccgcgc ttcctgctgc cgcttggcga actcgcggca tgtctcggtg aggccggtga
     2521 ggtcgcgcag ggcctcgcca ccgtcgacga ggcgctgctg cgctgcgaag ccagggacga
     2581 acgatggtac gtggctgagc tgctgcgcat caagggcgag ttgctgctgc acgaaaccga
     2641 acatcgatcc attccggccg ccgaacagtg tttcggcgaa gcgcttgaat tggcccggcg
     2701 tcaaggcgcg ctgttttggg agctcaggag cgcgttgagc cttgcccgac tgaggatcgg
     2761 gcaggaccgg cgggccgacg cgcgagcggt cctcgcgccg gcctacgaaa aattcgctga
     2821 aggcttcgag actgccgatc tgcgcaacgc aggagcgatg cttgagcagc tttcggcttc
     2881 gtaagttctc cgcaatgtcc agttcactca cagcagcggt catcccaaac cgttccgaac
     2941 ccgtcattgc gagcgtagcg aagcaatcca gagatggata cgagagttat ggattgcttc
     3001 gtcgcttcgc tcctcgcaat gacgatgcgg ctggatcagg tttttgtctt tttgaccgcc
     3061 ttcttggcag gctgagcctc tgccgcgttt cgcatcgatc tcgcgtagct gtccgcggcg
     3121 ttatcggcgg aaatctgcag ccgcttggcg gcggcggttc cttgcaacat gatattcttc
     3181 gcagtctgtc gatatttttc cttggtgtcc ttgtcctttg ccttggcggc gaggctgaca
     3241 tatcggtcgc gccgacgttt aacggcggtc atgagaccct tattctgttg cttcgcaagt
     3301 tgccggatta cgatatccag atcggcttcc gccatttttt gtttcctcag gtgccatcac
     3361 aacattccac accatcaagt atgcaattgc tgagcgcgct ttgcaattat gcggcgggcg
     3421 aatgcgctat ctggggaata gttcttggat cacttggccg aggccgattg ttcggatggt
     3481 ttgccgaaaa cgctgcgaca accttcgctc aggctggccc gctgcttgcg catgcaggcg
     3541 gtaatacgct ccacactcgg aatttccgac gcgcaaagtc gaaaaacatc gctggtgcaa
     3601 agccggcgtt gatcgtcttg cgtaaatgcg aggctcgtcg ccggcagcac gaaacagaaa
     3661 gccaaccccg atgcgatctt gagattgcgg agtgtgaaca gatcaattgg catagtcgcc
     3721 ccctggattg accgacgttg cggccgaccg ccagacggtt ggccgccttc gaatcaattc
     3781 atgattgtat tgacatgaat gccaatgctt caagactctc aaccaccgtt gcgccaaatt
     3841 ttgtatcggc gagcacagcg ccatcgccag cccatgtgga gcagc
//
LOCUS       DATNOI010000524        12652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_135114,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000524 DATNOI010000000
VERSION     DATNOI010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12652
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135114"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VN968_21195"
     CDS             complement(<1..1181)
                     /locus_tag="VN968_21195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HXL31788.1"
                     /translation="MSIVINPNIRSAAAQDVTAPLVLQPGSVISAKVQQVLGNDTVRI
                     AIGDQSIDVLSQVPLQAGQTLQLAVSQSPDGTIRLAVVNPQGGTAASQGAGDPAGAAA
                     TADAVTSAPDAAASIAAQKTSSVAAPTNQLTTLETLAVSVAAQTAATRQTSLAPLFAN
                     LSVAAGLDGLPAPVQQAVGQLLAQRTSLDQGLTGNDIKQAFQNSGLFLEASLASGSLS
                     PSSSSTPDLKAALIVLRQVLTTSLNGATPTLGALATPGTAATPSTPATGSAPTPAAAI
                     VPQATTSPTPVVQGAMTPVATALAQATLLSIQGSMQPVVITVGPETPTMASPALVPLL
                     VPEISAFGATLQAAAPPVPQDVPGFGAANPVSPTASMPVDAALATASAAALNLLQEAL
                     QA"
     gene            complement(1236..2021)
                     /locus_tag="VN968_21200"
     CDS             complement(1236..2021)
                     /locus_tag="VN968_21200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HXL31789.1"
                     /translation="MRELFDEVAGKSPLDPEEAVRRTTRGPRRKRFYAHAGVVDAPDG
                     LAITLDDKSVRTPSGRPLVAPTREIAAAIVTEWDSQKEFIDPLTMPLTRFANSVADAV
                     VDRVEAVADDIAKYLGSDLLFYRAGHPEALVAREAALWDPVVFWAAEALGAHFILAEG
                     IVHVRQPDSAIAAARAALPADPWSIAALHVVTTLTGSALLALALLAGVLDSDEVWAAA
                     HVDEDWNIEKWGVDEEVAARRAARLVDFRAAGRILKSLNSAAG"
     gene            2116..2565
                     /locus_tag="VN968_21205"
     CDS             2116..2565
                     /locus_tag="VN968_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31790.1"
                     /translation="MARVFATILVLLAGFGLSTVEAGFRSPESLARNVYAYYGDRSSE
                     LSSGLPRDPDTASRFFDPGLRAAWISVKDQPYDFLVQSPTWKLGAVSISILRRQFDKT
                     YVAVAFDNNGRAVALNFIVVNGPDGWVILDVESPHDSLRMFLAQYRH"
     gene            complement(2577..2885)
                     /locus_tag="VN968_21210"
     CDS             complement(2577..2885)
                     /locus_tag="VN968_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31791.1"
                     /translation="MKNLSILICAVAAALVAADAALAQVIIPPGGSQFNPPLPPPPPP
                     PKIEPPVIPQLDVPLRQNYAPAPRPSFSDRISRCLDEGAASGLGPNERAAYSRSCANQ
                     "
     gene            complement(3018..4241)
                     /locus_tag="VN968_21215"
     CDS             complement(3018..4241)
                     /locus_tag="VN968_21215"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL31792.1"
                     /translation="MIGNLLRAALFLFLFSLAACFSVAANAQTLALVGGRVYPSPDAA
                     PLVDAVVLTSGRAITAIGSRSEVQVPNDARVIDCTGKTVVAGFWNSHVHFTQAVWKNA
                     AGAPVASLEEHMREMLTRWGFTTVWDLGSDPNDSLPLRRRVNSGEVAGPNILLAGDIF
                     PKGGHPVYLPPEMQLPEAATSEEAAQMARDDLRMGLDGMKLFTGAYMGNKPVVNMDAA
                     IAKAAVDVAHAQGKPVFAHPQNRTGVDTVITAGVDVLAHIVPGEPSYSPEQLARFKSQ
                     GIALIPTLSLFATSVGGPALAARLVESGVNQLKGFSENGGPVLFGTDVGFTTVYDTSL
                     EYELMHRALSESQVLASLTTNPALYFKAAKKGKVEQGFDADLVVLDGDPIADVRNLAR
                     IAYTIRAGQVIYHRP"
     gene            complement(4396..5607)
                     /locus_tag="VN968_21220"
     CDS             complement(4396..5607)
                     /locus_tag="VN968_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXL31793.1"
                     /translation="MSRRVKRPLAKSRAPGDRPKGARPFRGSQAERQPGHRAGGKPAP
                     RFAASPPGAPRPAPFIEAPEKIVAEPPPLPTKVQTVVVTADENNMRVDRFLEARFPGL
                     SFSHIQRIVRKGELRVNGKRADSKDRLEEGQNVRVPPLKLDAPKVAGSLSEAATKTLQ
                     ALKDMILYEDADVMVLNKPAGLAVQGGSGTTRHVDQMLEVMRDAKGQKPRLVHRLDKE
                     TAGCLLVAKTRFAATALTGSFRHRSARKIYWALVAGVPKPKQGRISTYLAKDESEDDS
                     IMRIAAHGDEGASHAVTYYAVVETSAQKLAWVSLKPVTGRTHQLRAHMAHIDHAIVGD
                     PKYFNKENWQLPGGLQKRLHLLARRIVIPHPRGGFIDATAPLPPHMLQSWNLLGLEAD
                     RFDPIENAPEE"
     gene            complement(5604..6935)
                     /locus_tag="VN968_21225"
     CDS             complement(5604..6935)
                     /locus_tag="VN968_21225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HXL31794.1"
                     /translation="MSPKRPREATNLFAAAGMEQDAPHPLPDRLRPRALSDVVGQDHI
                     LGPDGALTRMLATRTLGSLIFWGPPGTGKTTVARLLADATELHFEQISAVFSGVADLK
                     KVFDAARARREMGKGTLLFVDEVHRFNRAQQDSFLPVMEDGTVVLVGATTENPSFELN
                     AALLSRARVLVFHSLDPAAIEKLFAHAEKIEARKLPLDAEARAVLIRLADGDGRAALT
                     LAEEVWRAAREGETFNAEALQDILQRRAPIYDKSADGHYNLISALHKSVRGSDPDAAL
                     YYLARMMDAGEDPLFLARRVVRMAVEDIGLADPQALVICNAAKDAYDFLGSPEGELAI
                     AQAVIYLATAPKSNAAYKAFGAAMRAAKEGGSLLPPKHILNSPTKLMKSEGYGSGYEY
                     DHDTQDAFSGQDYFPEAMGRQTFYDPPDRGFEREIRKRLDYWAKLRNGKTS"
     gene            complement(6932..8326)
                     /locus_tag="VN968_21230"
     CDS             complement(6932..8326)
                     /locus_tag="VN968_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HXL31795.1"
                     /translation="MMLIRFLAVICLSAMAAMPAQAQDRRVPASPAEIKLSYAPIVQR
                     VQPAVVNVYAAKVVQNRNPLLDDPIFRRFFGVPGQQPEQMQRSLGSGVMVDPSGLVVT
                     NVHVIEGADEVKVSLSDKREFEAEIVLKDPRSDLAILRLKDAKEKFPTLDFANSDELL
                     VGDMVLAIGNPFGVGQTVTHGIISALARTQVGITDYQFFIQTDAAINPGNSGGALVDM
                     TGRLAGINTAIFSRSGGSQGIGFAIPANMVRVVVASAKSGGKAVKRPWLGARLQAVTP
                     EIAESLGLRLPTGALVASVVASSPAARSGLKASDLIVAIDGQAVDDPNGFDYRFATHP
                     LGGTSQVDVQRAGKTLKLTIPLETAPDTGRNEIVLTTRSPFQGAKVANISPAVADELH
                     LDADIEGVVVTDLADGATAASVGFQKGDIIMSVNNQKIARTSDLEKATRDSVRLWRIT
                     VVRGGQQINVTLGG"
     gene            complement(8399..8767)
                     /locus_tag="VN968_21235"
     CDS             complement(8399..8767)
                     /locus_tag="VN968_21235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL31796.1"
                     /translation="MKRRNFAHRPGCAVEATLDLIDGKWKGVILYHLQDGTQRFGELR
                     RRMPGITQRMLTKQLRALEDDQLVIRKVYAEVPPRVEYRLSEIGESLRPVIDTLKAWG
                     EGHRQKLSCAPVPDVPDRAA"
     gene            9015..10028
                     /locus_tag="VN968_21240"
     CDS             9015..10028
                     /locus_tag="VN968_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HXL31797.1"
                     /translation="MKAVGYKKSLPVDEADSLIDFETVKPEPKGRDIRVAVKAISANP
                     VDYKVRKRAAPPEGETKILGYDAAGIVDAVGPEVTLFKPGDEVFYAGSIQRQGTNSEF
                     HLVDERIVGRKPKTLSFAQAAALPLTSITAWELLFDRLGALPGKSVDPRTLLIIGGAG
                     GVGSILIQLARRLTGLTVVATASRPESQTWCIDLGAHAVIDHAKPMKEQIEKLKLPPV
                     ALIASLTNTDQHYKALADLIAPQGKYGLIDDPPEFNVSVFKGKAVSIHWESMFTRSSF
                     QTPDMIGQHALLNDVADLIDKGVLRTTLDQTFGTINAANLKRAHALLESGKSRGKIVL
                     EGW"
     gene            complement(10032..10328)
                     /locus_tag="VN968_21245"
     CDS             complement(10032..10328)
                     /locus_tag="VN968_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HXL31798.1"
                     /translation="MSAYVISEVDVRDPAGFEAYRTIAAKAIAQYGGRYLVRGGAADI
                     AEGGPPPKNIIVVEFPSMEKLREWYASPEYAEALKVRRTALDRRLIFVEGVPAS"
     gene            complement(10337..11131)
                     /locus_tag="VN968_21250"
     CDS             complement(10337..11131)
                     /locus_tag="VN968_21250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL31799.1"
                     /translation="MNIDLKGKTALVTGSTSGIGHAIVKGLAVAGANVVVNGRTQAKV
                     DTAVAAVAKAAPGGRIAGVAADVSTAAGCDTLVEMLPDVDILINNAGIFEPKGFFDIA
                     DDDWSRFFEVNVMSGVRLSRAYLPGMLKRNWGRIVFISSESALNIPKEMIHYGMSKTA
                     QLAVSRGLAEMTRGTAVTVNSVLPGPTMSEGVEAFVKDLAKQNGQSVDEAASQFVKQF
                     RPTSLLQRFASVEEIANMVVYVSSKEASATNGAALRAEGGIVQTIA"
     gene            complement(11270..11686)
                     /gene="rplQ"
                     /locus_tag="VN968_21255"
     CDS             complement(11270..11686)
                     /gene="rplQ"
                     /locus_tag="VN968_21255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HXL31800.1"
                     /translation="MRHGKVHRKLNRTAEHRRAMFANMCAALIKHEQIVTTLPKAKEL
                     RPIVEKLVTLGKKGGLAMRRQAISEMRDLDQTRKLFDVLATRYKDRHGGYTRIIKAGF
                     RYGDNAAMAVIEFVDRDVDAKGLDSGPAQEKAAEAA"
     gene            complement(11827..>12652)
                     /locus_tag="VN968_21260"
     CDS             complement(11827..>12652)
                     /locus_tag="VN968_21260"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HXL31801.1"
                     /translation="DGVLHEFSSIAGVREDVTDIVLNIKDISIKMQGEGPKRMVVKKQ
                     GPGVVTAGDIQTVGDVVVLNPDLQICTLDEGAEIRMEFTVAAGKGYVAAERNRPEDAP
                     IGLIPVDSLFSPVRKVSYKVENTREGQILDYDKLTMTIETNGAISPDDAVAYAARILQ
                     DQLNVFVNFEEPRKEVAQEIIPDLAFNPAFLKKVDELELSVRSANCLKNDNIVYIGDL
                     VQKSEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGMEVPGWPPENIDELAKRFEDHY"
BASE COUNT         2302 a         4068 c         3950 g         2332 t
ORIGIN      
        1 gcctgcaggg cttcctgcag caaattgagc gctgccgcgc tggcggtggc aagcgcggca
       61 tccaccggca tcgaagcggt cgggctgacc ggattcgcag cgccgaagcc gggcacatct
      121 tgcgggaccg gcggcgccgc ggcctggagc gtagctccaa aggccgagat ctccggcacg
      181 agcagcggca ccagcgcggg cgatgccatc gtcggcgttt ccggcccaac cgtaatgacg
      241 acgggctgca ttgagccttg aatggacaac agcgtggcct gcgccaacgc agttgccacc
      301 ggcgtcattg ccccctgcac caccggcgtt ggcgacgtcg tggcttgcgg aacgatggcg
      361 gccgccggtg tcggtgcgct gcccgtcgcc ggcgtgctcg gcgtcgccgc cgtgcctggc
      421 gtcgccaacg cacccaaggt cggcgttgcc ccgttgagcg acgtcgtcag cacctggcgc
      481 agcacgatca gcgccgcctt gagatccggc gtggaggatg aggacggcga aagcgatccg
      541 gacgccaatg aggcttccag aaacaggccc gaattctgaa acgcctgctt gatgtcattg
      601 ccggtcaggc cttgatcgag gctggtccgc tgcgccagca gttgcccgac cgcctgctgg
      661 accggcgccg gcaggccatc cagcccggcg gcaacgctta agttggcaaa cagtggcgcg
      721 agactggtct gccgggtcgc cgcggtttgc gccgcgaccg ataccgcgag cgtttccagc
      781 gtcgtgagct gattggtcgg tgccgcaacc gatgaggtct tttgtgcggc gatgctggcg
      841 gccgcatcgg gggccgaggt cacggcatcc gctgtcgcgg ccgcgccggc aggatcgccg
      901 gcaccctggc ttgccgcggt gccgccctgc gggttcacca ccgccagcct gatcgtaccg
      961 tccgggctct gcgaaaccgc aagctgcaac gtctggccgg cctgcagcgg gacctgcgaa
     1021 agtacgtcga tcgactggtc gccgatcgcg atccgcaccg tgtcgttacc gagaacctgc
     1081 tgcaccttgg cgctgatcac gctgccgggc tgcagcacga gtggcgccgt cacgtcctgt
     1141 gcggcggcgg accgaatgtt cggattgatg acgatcgaca tgggcttcac cgggtcaggc
     1201 ccggtcgaag ctatccgggt gtcgtaaacc tgccgttaac ccgccgcgga atttagcgac
     1261 ttgaggatgc gcccggcagc gcgaaaatcg accagccgcg ccgcgcggcg ggccgccacc
     1321 tcctcgtcca ccccccattt ttcgatgttc cagtcctcgt cgacatgggc tgcggcccag
     1381 acctcatcgg aatcgagcac gccagccagc agcgctaagg cgagcagcgc cgagcccgtc
     1441 aatgtcgtca ccacgtgcaa ggctgcgatc gaccaggggt ccgccggcag cgccgcgcgc
     1501 gccgccgcga tcgcggaatc cggctggcgc acatgcacga tcccttcggc caaaatgaaa
     1561 tgcgcaccca gcgcctccgc agcccaaaac accaccggat cccaaagtgc tgcttcccgc
     1621 gccaccagcg cctccggatg gccggcgcgg tagaacaaaa gatccgaccc gagatatttc
     1681 gcgatgtcgt ccgcgaccgc ttcgacccga tccaccacgg catcagcgac actgttggcg
     1741 aaccgggtca acggcatggt caggggatcg atgaattcct tctggctgtc ccattccgtt
     1801 acaatagcgg cggcgatctc acgggtcggc gcaaccagcg ggcgcccgga aggcgtgcgg
     1861 accgacttgt catcgagggt aatggccaaa ccatccggcg cgtcgacgac gcccgcatgg
     1921 gcgtaaaaac gcttgcggcg cggcccgcgc gtggtgcgcc gtaccgcctc ctccggatcg
     1981 agcggagact ttccggcgac ttcatcgaac agttcacgca ttcggttttc agcttatgtc
     2041 cgtcaatggt aatttgaccg ccagcgtacg acaaggatgg ccgcaaataa agcgagctgg
     2101 ccaagcgagc ctgcaatggc gagggttttc gcaaccatcc tggttctact ggccggcttc
     2161 ggtctctcga ccgtcgaggc tggtttccgt tcgccggaat cgctggcgcg gaacgtgtac
     2221 gcctattacg gcgacaggtc gtccgaactc tcgagcgggc tgccgcgcga ccccgacacc
     2281 gcaagccggt tcttcgatcc aggtttgcgg gcggcatgga tttcggtgaa ggatcagccc
     2341 tacgatttcc tggtgcagag cccgacctgg aaacttggcg ccgtctcgat atcgatcctg
     2401 cgcaggcaat tcgacaagac ctatgtcgcc gtcgccttcg acaataacgg ccgcgccgtc
     2461 gcgctgaact tcatcgtggt gaatggtccc gacggctggg tcattctgga cgtcgagagc
     2521 ccgcatgatt cactgcgcat gttcctcgcc caataccggc actaagagag ttcctctcat
     2581 tgattggcgc aggaccgcga ataggccgcg cgctcgttcg gaccgagccc gctggcggcg
     2641 ccttcatcga gacatctgct gatccggtcg ctgaacgaag ggcgcggcgc gggcgcatag
     2701 ttctggcgca acggcacgtc gagctgcggt atcaccggcg gctcgatttt gggcggcggc
     2761 gggggtggag gcagcggcgg attgaattgc gacccgccgg gcggaatgat cacctgcgcg
     2821 agcgcggcat ccgcggcaac cagcgcggct gcgaccgcgc agatgaggat cgataaattc
     2881 ttcatggagg gtatgtcgtg atcctcccgg caagttgcaa gctgaaatta ttcttccggc
     2941 tcgttctcga tcggatgacg gcaatggccg cgcgctcaca ctggatgacg ggccaattcg
     3001 ggaacggcag cagcctttca cggtctgtga taaatgacct ggccggcacg gatggtgtag
     3061 gcgatcctgg cgaggttgcg aacgtcggcg atgggatcgc catcgagaac cacgagatcg
     3121 gcgtcaaatc cttgttcgac cttacccttc ttcgcggcct tgaaatacag agcggggttc
     3181 gtcgtgaggg aagccagcac ctggctttcc gaaagcgccc ggtgcatgag ctcgtattcg
     3241 agcgatgtgt cgtagaccgt ggtgaagccg acgtcggtcc cgaacagaac ggggccacca
     3301 ttttccgaaa accccttgag ctggttgacg ccggactcga ccagccgggc tgccaaggcg
     3361 ggaccgccga cactggtcgc aaaaagagac agcgtcggaa tcaatgcgat gccttgcgat
     3421 ttgaaccggg cgagttgctc ggggctgtaa ctcggttctc cgggaacgat gtgggcgagc
     3481 acatcgactc cggctgtaat cactgtgtcc acaccagttc tgttttgcgg atgggcgaac
     3541 accggcttgc cttgtgcatg tgccacatcg accgccgcct tggcgatcgc ggcatccata
     3601 ttcacgacgg gcttgttgcc catgtacgcg ccagtaaaaa gtttcatgcc gtccaggccc
     3661 atgcggaggt catcgcgcgc catttgcgcg gcttcctctg atgtagcggc ctcggggagc
     3721 tgcatctcgg gaggcaaata gacgggatga ccgcccttgg ggaagatgtc gccggcaaga
     3781 aggatattgg gacccgcgac ctcaccggag ttgacgcggc ggcggagcgg caacgagtcg
     3841 ttcgggtcgg agccaagatc ccaaacggtg gtgaaacccc accgcgtcag catctcccgc
     3901 atatgctctt cgagcgacgc gaccggcgcg ccggccgcgt ttttccacac cgcttgcgtg
     3961 aaatggacgt gactattcca gaagccggcg acgacagtct ttccagtgca gtcaatgacc
     4021 cgcgcatcgt tcgggacctg gacttcgctg cgactgccga tcgcggtgat ggcgcgccca
     4081 gacgtcagga cgacggcatc cacgagcggc gcggcatcgg gcgacgggta taccctgcct
     4141 cccacgaggg cgagtgtctg cgcgttcgcg gcgacgctga aacaagccgc caggctgaag
     4201 aggaaaagga aaagcgcggc gcggagcaga tttccgatca tgggcggagc cttgaacttt
     4261 gcatgtcggc gcctcgaagc gttgaggaaa ggcgcccgtc ggaccgaacg atatccgatc
     4321 agtacgacct cgctttgata gttcttgctc ctcccggacg atatcgggtt gcggccattg
     4381 ctgtcgcgac gcccgtcatt cttccggcgc gttctcgatc gggtcgaacc tgtcggcttc
     4441 caggcccagc aggttccatg attgcagcat atggggcggc agcggcgcgg tggcgtcgat
     4501 gaatccgccg cgcggatgcg ggatcacgat ccggcgtgcc aaaagatgca gccgtttctg
     4561 caggcccccc ggcagctgcc agttttcctt gttgaaatat ttgggatcgc cgacaatggc
     4621 gtgatcgata tgggccatgt gcgcacgcag ctggtgggtc cgcccggtga ccggtttcaa
     4681 tgacacccag gccagcttct gcgccgaggt ttccaccacg gcataatagg tgacggcatg
     4741 gctggcgccc tcgtcgccat gtgcggcgat ccgcatgatg ctgtcgtcct cgctctcgtc
     4801 cttggcgaga taggtcgata tgcggccctg cttcggcttc ggtacgccgg ccaccagcgc
     4861 ccagtagatc ttgcgcgcgg agcgatgacg gaacgatccg gtcagcgccg tcgcggcgaa
     4921 ccgcgttttc gcaaccagga gacagcccgc cgtctccttg tcgagccggt gcaccagccg
     4981 cggcttctgt cccttggcat cgcgcatcac ttcaagcatc tggtcgacat gccgcgtggt
     5041 gccggaaccg ccttgtaccg caagccccgc gggcttgttc agcaccatga catcggcatc
     5101 ctcgtagagg atcatgtcct tgagggcttg cagcgtcttt gtcgccgctt ccgaaagact
     5161 tccggccacc ttgggcgcat cgagtttcag cggtggaacg cgaacgtttt gcccttcctc
     5221 aagccggtcc ttgctgtcgg cgcgcttgcc gttgacgcgc agctcacctt tgcggacgat
     5281 gcgctggatg tgggagaacg acaggccggg aaaccgcgcc tcgagaaacc gatccacgcg
     5341 catgttgttc tcgtccgccg tcacgaccac ggtctgcact tttgtcggca gcggcggcgg
     5401 ctcggcgacg atcttttcgg gcgcctcgat aaacggcgcc ggcctcggcg cacccggcgg
     5461 cgatgccgca aaacgcggcg caggtttacc gcccgcacga tgccccggct gtcgctcggc
     5521 ttgcgagccc cggaagggcc gcgcgccctt ggggcgatcg cccggcgcgc gggatttcgc
     5581 cagtggtctc ttgacgcgac ggctcatgat gtttttccgt ttcttagctt cgcccagtaa
     5641 tccagccgtt tgcgaatttc gcgctcgaag ccgcggtcgg gcggatcgta gaaggtctgg
     5701 cgtcccatcg cttccgggaa ataatcctgg ccggagaacg catcctgggt gtcgtgatcg
     5761 tattcgtagc ccgagccata gccctccgac ttcatcagct tggtcggcga attcaagata
     5821 tgctttggcg gcagcagcga gccgccctcc ttggcggcgc gcatcgccgc gccaaaggcc
     5881 ttgtaggcgg cgttcgattt cggcgcggtg gcgagataaa tcaccgcctg cgcgattgcc
     5941 agttcgcctt cgggagaccc caggaaatca taggcgtcct tggcggcgtt gcagatcacc
     6001 agcgcctgcg gatcggcgag cccgatgtcc tcgaccgcca tgcgaacaac gcgccgcgcc
     6061 agaaacagcg gatcctcgcc ggcgtccatc atccgcgcga gataatacaa agcagcatca
     6121 ggatcggagc cgcgcaccga tttatgcagc gccgagatca ggttgtagtg gccgtcggcg
     6181 gacttgtcgt agatcggcgc ccggcgctgc aggatgtcct gcaaggcttc cgcattgaag
     6241 gtctcgcctt cgcgcgcggc gcgccagacc tcttcggcca gcgtcagcgc ggcgcgcccg
     6301 tcgccatcgg ccaggcgtat cagcacggcg cgcgcctccg catcgagcgg cagctttctg
     6361 gcttcgatct tctcggcatg ggcgaacagt ttttcgatcg ccgccgggtc gagcgaatga
     6421 aacaccaaaa cccgcgcccg cgacagcagc gcggcgttga gctcgaacga gggattttcg
     6481 gtggtcgcgc cgaccagcac cacagtgccg tcttccatga caggcaaaaa cgaatcctgc
     6541 tgggcgcggt tgaaccgatg cacctcatcg acgaacaaga gtgttccctt gcccatttcg
     6601 cggcgtgcgc gggccgcgtc gaaaactttt ttcaggtcgg cgacaccgga aaacaccgcg
     6661 gaaatctgct cgaaatgcag ttcggtcgca tccgccaata atctcgcaac ggtggtcttg
     6721 ccggttcccg gcggccccca gaaaatcagc gaacccagcg tccgcgtggc gagcatgcgc
     6781 gtcagcgcgc cgtccggccc aagaatatga tcctgcccca cgacatccga caaggcgcgg
     6841 gggcgcaacc ggtccggaag cggatgcggt gcatcctgct ccatgcctgc cgcagcaaac
     6901 aggttggtgg cttcgcgtgg tcgcttcggg ctcatccgcc cagcgtcacg ttgatctgct
     6961 ggccgccgcg caccacggtg atgcgccaaa gccgcacaga gtcgcgtgtc gccttctcaa
     7021 gatcgctggt cctggcgatt ttctgattat tgaccgacat gatgatgtcg cccttttgga
     7081 acccgacgct ggcggcggtg gcgccatccg ccagatcggt caccacgacg ccttcgatgt
     7141 ccgcatccag atgcagctca tcggcgaccg cgggcgagat gtttgcaacc tttgcgccct
     7201 ggaatggcga acgcgtcgtc aggacgattt cgttgcggcc ggtatcgggc gccgtctcga
     7261 gcggtatcgt cagcttcagc gtcttgccgg cgcgctgcac atcgacctgc gacgtgccgc
     7321 cgagcggatg tgtcgcaaag cggtagtcga acccattggg atcgtccacc gcttgtccgt
     7381 cgatcgcgac aatcagatcg gatgccttca gcccggaccg cgctgccgga ctgctcgcaa
     7441 ccacactggc gaccagcgcg ccggtcggaa gccgcagccc caggctctcg gcaatctccg
     7501 gcgtcaccgc ctgcaaccgt gcgccaagcc agggccgctt gacggccttg ccgccgctct
     7561 tggcggaagc gacgacgacg cgcaccatgt tggccggaat ggcaaagccg atgccctgcg
     7621 acccgccgga acgcgagaag atcgcggtat tgatgccggc cagtcggccg gtcatgtcga
     7681 ccagggctcc gcccgaattg ccggggttga tcgcggcatc ggtctggatg aagaactggt
     7741 agtcggtgat gccgacttgc gtgcgcgcca gcgccgaaat gatgccgtgg gtaacggttt
     7801 gcccgacgcc gaagggattg ccgatcgcaa gcaccatgtc gcccaccagc agttcgtcgg
     7861 agttggcaaa gtcgagcgtc gggaatttct ctttcgcgtc tttcaggcgc aggatggcca
     7921 gatcgctgcg gggatccttg agaacgatct cggcttcgaa ttctcgcttg tccgacagcg
     7981 aaaccttgac ctcatcggcg ccttcaatga catggacgtt ggtcacgacg agaccggacg
     8041 ggtccaccat cacgcccgag cccagcgagc gctgcatctg ttccggctgc tggcccggca
     8101 cgccaaagaa acggcggaag atcgggtcgt cgagcagcgg attgcggttc tgcaccacct
     8161 tggcggcata gacgttgacc accgccggct gcacgcgctg cacgatcggc gcgtaggaaa
     8221 gttttatctc ggccggcgat gccgggacgc gccggtcctg cgcctgcgcc ggcatcgcag
     8281 ccattgcgga aaggcagatc acggcgagga agcgaatcaa catcatgcga aaatcctgga
     8341 attcgtcgcc tcagatatag tggcgaggca actgcgagag aagggaaacc cggatcagct
     8401 aggcggcgcg atcggggaca tccggcaccg gcgcgcagga tagcttctgc ctgtggcctt
     8461 cgccccacgc cttcaaggtg tcgatcaccg gacgcaggct ttcgccgatt tccgagagac
     8521 gatattcaac ccgcggcggt acttcggcat agaccttgcg gatcaccagc tggtcgtcct
     8581 ccagcgcacg gagctgcttg gtcagcatgc gctgcgtgat gcccggcatc cgccgccgca
     8641 actcgccaaa gcgctgggtg ccgtcctgca aatggtagag gatgacacct ttccacttgc
     8701 catcgatcag gtcgagcgtc gcttcgaccg cgcagcccgg ccggtgggca aaattccgtc
     8761 gtttcatggg gcattttcca atagtatcca aacggggact atatccccga atttacagta
     8821 cttgccaata tcgcgccact ccgacaatta aagccaaagc gttttcaagc gaaagcctgc
     8881 cctcggactt gatccggggg tgggtaccgg ttcgcgtgaa gaaaacgcgt caaatcgaga
     8941 accagcgcct ctggttctga ttatcagaac cgaggcggct gacgatttga gccatcacag
     9001 aggagagcac gggcatgaag gccgtcggat acaaaaagtc gcttcccgtc gatgaggcgg
     9061 actcgctgat cgatttcgag accgtaaaac ccgaaccgaa ggggcgcgat atcagggtcg
     9121 ccgtaaaggc gatttcagcc aatccggtcg attacaaggt gcgcaagcgc gcagcacccc
     9181 cggaaggtga aaccaagatt ctcggctacg acgccgccgg gatcgtcgac gcggtcggac
     9241 cggaagtgac gctgttcaaa cccggcgacg aggttttcta tgccggctcg atccagcgcc
     9301 agggcaccaa ctcggaattt catctggtgg atgagcgcat tgtcggccgg aagccgaaga
     9361 cgctgtcgtt cgcgcaagcc gccgccctgc cgctgacctc gatcaccgcc tgggaattgc
     9421 tgttcgaccg tctcggcgcg ctacccggca aaagcgtcga tccgcgcacg ttgctgatca
     9481 tcggcggggc cggcggcgtc ggctcgatcc tgatccagct ggcgcgccgc ctcaccggcc
     9541 tgacggtggt ggcgacggcg tcccggccgg agtcacagac atggtgcatc gatctcggcg
     9601 cgcatgccgt catcgaccac gccaagccga tgaaggaaca gatcgaaaag ctgaaactgc
     9661 cgccggtcgc gctgatcgcg agcctcacca acaccgacca gcactacaag gcgctggccg
     9721 acctcatcgc gccgcaaggc aaatacggct tgatcgacga tcccccggag ttcaacgtca
     9781 gcgtcttcaa gggcaaggcg gtctcgatcc actgggaatc gatgtttacg cgaagttcgt
     9841 tccagacgcc ggacatgatc ggccagcatg ccctgctcaa cgacgtcgcc gacctcatcg
     9901 acaagggggt gctgcgcacc acgctcgacc agacctttgg gacgatcaac gcggccaacc
     9961 tgaaacgtgc gcacgcgctg ctggaaagcg gcaagtcgcg cggcaagatc gtgctggagg
    10021 ggtggtagca gtcacgacgc gggcacgccc tcgacgaaga tcaggcggcg gtcgagcgcg
    10081 gtccgccgca ctttcagcgc ctcggcgtat tccggcgagg cgtaccattc gcgcagtttc
    10141 tccatcgagg gaaattcgac cacgatgatg ttcttcggcg gcgggccgcc ctccgcaata
    10201 tcagccgcgc cgccgcggac cagatagcgg ccgccatatt gcgcaatcgc tttcgcagca
    10261 atggtgcgat aggcctcgaa acccgcgggg tcgcgcacat caacttcgga aatgacataa
    10321 gccgacatct gacatctcac gcaatggtct ggacgatacc gccctcggca cgcagcgccg
    10381 cgccattggt cgcggatgcc tctttcgagg agacatagac caccatgttg gcgatctcct
    10441 ccacgctcgc aaagcgctgc agcagcgagg tcgggcgaaa ctgtttgacg aactgcgacg
    10501 ccgcctcgtc caccgactgg ccgttttgct tcgcgaggtc cttgacgaat gcttcgacgc
    10561 cctccgacat cgtcggcccc ggcagcaccg aattgacggt taccgcggta ccgcgggtca
    10621 tctcggcgag gccgcgcgat accgcgagct gcgcagtctt ggacatccca taatggatca
    10681 tttccttcgg aatattcagc gcggattccg aggagatgaa gacgatgcgg ccccagttgc
    10741 gcttgagcat gcccggcagg taggcgcgcg acagccgcac gcccgacatc acattgacct
    10801 cgaagaagcg gctccagtcg tcgtcagcaa tatcgaagaa gcctttgggc tcaaaaatcc
    10861 cggcattatt gatgagaatg tccacatccg gtagcatctc aaccagcgtg tcgcatcctg
    10921 cggcggtcga aacatcggca gcgacgcccg cgatcctgcc gccgggcgcg gccttggcaa
    10981 ccgcggccac ggccgtgtcg accttggcct gggtccggcc gttgaccacc acattcgccc
    11041 cggcaacggc gaggcctttg acgatcgcgt ggccgatgcc ggaggtggac cccgtcacca
    11101 gcgccgtctt tcccttgaga tcgatattca tgaggcatct ccatcattga tggcggagat
    11161 atcggaagcg gcccgcgcca ccgcaatcgc cgttcaaccg taaagaacgc ccaataaaaa
    11221 agcggcgcgc atggcaccgc tttttggaat aaagttccgg agtggggttt tacgcggctt
    11281 ccgcggcctt ttcctgcgcc ggaccggaat cgaggccctt ggcatcgacg tcacggtcga
    11341 cgaattcgat caccgccatg gccgcgttgt ccccgtagcg gaagccggcc ttgatgatgc
    11401 gggtatagcc gccatgccgg tccttgtagc gggtggccag cacgtcgaac agtttcctgg
    11461 tctggtcgag gtcgcgcatc tccgagatcg cctgccggcg catcgccaga ccgcccttct
    11521 tgccaagcgt aaccagcttt tccacgatcg ggcgcagttc cttcgccttc ggcagcgtgg
    11581 tgacgatctg ctcgtgcttg atcagcgccg cgcacatatt ggcaaacatc gcgcgccgat
    11641 gttcggcggt gcgattgagc ttgcgatgaa ccttgccgtg acgcatggcg tctctcctgc
    11701 ctgaacccca gtcattccgg acgccgaagc ggcgatccgg aatctcgata tagcaactcc
    11761 gagattcccc gatgtgcaat tgcacatccg aggttcgcgc taccgcgcgc cccggaatga
    11821 cggaccttag taatgatcct cgaagcgctt ggccagctcg tcgatatttt ccggcggcca
    11881 gcccggcact tccatgccga ggtgcagccc catctgggcc agcacctcct tgatctcgtt
    11941 cagcgacttg cggccgaagt tcggggtgcg gagcatttcc gcttccgact tctgcacgag
    12001 gtcgccgata tagacgatgt tgtcgttctt caggcagttc gccgaacgca ccgacagttc
    12061 gagctcgtca accttcttga ggaatgccgg gttgaacgcc agatcgggaa tgatctcctg
    12121 cgccacttcc ttgcgcggct cttcgaagtt cacgaacacg ttgagctgat cctgcaggat
    12181 acgcgcggca taggccaccg cgtcatccgg cgagatcgcg ccgttggtct cgatggtcat
    12241 ggtcagcttg tcgtaatcga ggatctggcc ctcacgggtg ttctcgacct tgtacgacac
    12301 cttgcgcacc ggcgagaaca aactgtcgac cgggatcagg ccgatcggcg catcctcggg
    12361 acggttgcgc tcggcggcga catagccctt gccggcggcg accgtgaatt ccatgcggat
    12421 ttccgcgccc tcgtcgagcg tgcaaatctg cagatccgga ttgagcacca cgacatcgcc
    12481 gacggtctgg atatcgccgg cggtgacgac acccgggccc tgcttcttca cgaccatgcg
    12541 cttggggcct tcgccctgca tcttgatcga gatgtccttg atgttcagca cgatgtcggt
    12601 gacgtcctcg cgaacgcccg cgatcgagga gaattcgtgc agcacgccgt cg
//
LOCUS       DATNOI010000525         3032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2370321,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000525 DATNOI010000000
VERSION     DATNOI010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2370321"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VN968_21265"
     CDS             complement(<1..1212)
                     /locus_tag="VN968_21265"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007801266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL31802.1"
                     /translation="MTTILTKSFPQEKPPVTKAVPGKKRVLIVGGGFAGIAAARALKR
                     ADVEITLVDRRNHHIFQPLLYQVATAVLAPSEIAAPIRQLEAKQKNLSVLLAEIKGIN
                     LGARTVEAICPGIGGRKLEYDFLVIATGMRPSYFGHDEFARFAPGLKSLNDAETIRTK
                     ILSAFEVAESTDDPQERARQMTFVLVGAGPTGVELAASIAQLVSVTLRNNFRKIDLAK
                     SRILLLDGGARILPTFAESLSRKAAKRLTKLGVEVSTGAKVEKIDDQGVIAAGVRIPS
                     ATVLWTAGVSASPVVKMLGTKTDRAGRAFVGAFMDIPEAPNVFVAGDAATMTQDGHPV
                     PGVAQAAIQQGQFVGHVIANRVRGRKDGRPFRYRNKGNMAVVGKNFAILESGYLRSSG
                     FVTWLVWAALHV"
     gene            complement(1320..2384)
                     /locus_tag="VN968_21270"
     CDS             complement(1320..2384)
                     /locus_tag="VN968_21270"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HXL31803.1"
                     /translation="MPVSQIVPAKPLGVDMGRVLAVTTVIDQVCHGTLSAEAAQSALE
                     SAGRLPPASTPRFTLFAAIGAASLGVIFGTLDATSLLLIATSAAIGALVRRWLSGVSN
                     NPLIQPLCAAFIAGVVAATASRFQLSHAATLVAFCPCMVLVPGPHILNGAIDLARTRI
                     ALGIARLAYAGVIVLLICAGGLFGFTAVGANLAIAGPSPPVPLVIDVIAAGCAVASFG
                     TFFSMPWRLLAFPIAAGMLAHAARWALISIAGTNAATGALVACMLVSVLVTPIVDRQH
                     LPFAALAFSAVVSMMPGFFLFNAATGLVELVSIGPSAPAALLTSIATNGATALLIIMA
                     MTFGLILPRMLFERVLPSPA"
BASE COUNT          622 a          906 c          968 g          536 t
ORIGIN      
        1 cacgtgtagc gcggcccaca ccagccaggt cacaaagccg ctgctgcgca aataaccaga
       61 ctcgaggatg gcgaagttct tgccgaccac cgccatattg cctttgttgc ggtatcgaaa
      121 cggtcgacca tccttgcgtc ccctgacacg gttggcgatg acatgcccga caaattggcc
      181 ctgttgaatg gctgcctgcg ccactccggg gacggggtga ccatcctggg tcatcgtggc
      241 tgcgtcgccc gccacaaaca cgttgggcgc ctcgggaatg tccatgaaag cgccaacgaa
      301 agcgcgcccg gcgcggtctg tcttcgttcc gagcattttt acgacaggcg aggcgctgac
      361 gcctgctgtc caaagcacgg tggcgctggg aatccggacg ccagccgcga tcacgccctg
      421 gtcgtcaatc ttctcgacct ttgcgcccgt cgaaacttca acgccgagct tggttagtcg
      481 cttggcggcc tttcgtgaca gcgactctgc aaaggtcgga agaatacgag cgccgccgtc
      541 cagtaacaaa atgcggctct tggcaaggtc gatcttgcgg aaattgttgc gcagggtcac
      601 cgagaccagt tgagcgatcg aagcggcaag ctcgacgccg gtcgggccgg cacccaccaa
      661 cacgaaggtc atttggcgag cgcgttcttg cggatcatcg gtcgactccg ccacttcaaa
      721 cgcgctcagg atcttggttc ggatcgtttc agcgtcgttg agactcttga ggcctggcgc
      781 gaaccgcgcg aactcgtcat ggccgaaata gcttggacgc attccggtcg cgatcaccag
      841 gaagtcgtac tcgagttttc gaccgccaat ccccgggcaa atggcctcga cggtgcgagc
      901 tcccaggttg atgcccttaa tctcggccag caacacgctg aggttcttct gtttcgcctc
      961 aagctgtctg atcggcgccg ctatctcgga aggcgccaaa acagcggtgg cgacctggta
     1021 caggagcggc tgaaagatat ggtgattacg ccggtcgacc agcgtgattt cgacatcggc
     1081 tcgcttgagc gcgcgtgctg ccgcgatgcc ggcgaaaccg ccgccgacga tcaagacgcg
     1141 ttttttgccc gggaccgcct ttgttacagg cggcttttct tgcgggaacg atttggtcag
     1201 aattgtagtc atggatgtac caacaatctc tagggccagc cgcccgatta gcatcggata
     1261 cagcggggct tcgggctatt cttggacggc gctgcggtgc attgaacgat cgtgcctcat
     1321 catgcggggg agggcagtac gcgctcgaac agcatgcgcg ggaggatcag tccgaatgtc
     1381 atggccatga tgatcagaag tgccgtggcg ccgttcgtgg cgatgctcgt cagcagagct
     1441 gccggcgcgc tcggcccgat cgagaccaat tcgacaaggc cggtcgcggc gttgaacagg
     1501 aaaaagcccg gcatcattga gacaacggcg gagaaggcga gagccgcgaa gggcagatgc
     1561 tggcgatcga cgatcggcgt cacgaggacg ctgacgagca tgcaggcgac gagcgcgccg
     1621 gtcgcggcat ttgtccccgc gatggagatc agcgcccagc gggcggcatg cgcgagcatg
     1681 ccggccgcga tcgggaacgc gagcagacgc cacggcattg agaagaaagt gccaaaggag
     1741 gcgacggcgc atcctgccgc aatcacgtcg atgaccaagg gcaccggtgg agatggtccg
     1801 gctatcgcga ggtttgcgcc gaccgccgtg aacccgaaca ggccgccggc gcagatcagg
     1861 agcacgatga cgccggcata ggccaggcgc gcgattccca gcgcgatgcg cgtacgagcg
     1921 aggtcgatgg caccgttgag gatatgcgga ccaggaacca acaccatgca gggacagaaa
     1981 gcgaccagcg ttgctgcatg cgatagctgg aaacggctgg cagtagcagc cacgacacct
     2041 gcgataaacg cggcgcaaag cggctggatc agtggattat tgctgacgcc ggacagccag
     2101 cggcggacga gcgcaccgat agcggcgctg gttgcgatca gcagcaggct tgttgcatcg
     2161 agcgtaccga aaatgacgcc aagcgaagcc gcgccgatgg ctgcgaacaa agtgaagcgt
     2221 ggcgtggaag ctggcggcag ccggccagcc gactcaagcg cggattgcgc agcctcggcc
     2281 gacaacgtgc cgtggcagac ctggtcgatc accgtcgtga cggccagtac cctgcccatg
     2341 tcgacaccga gcggcttggc ggggacgatt tgtgagacgg gcacgccgtc gagttcgacg
     2401 gtgagttcgc cccactaagg tagcaccctg accgtcacgc cgagagcgcg cccaaggcgc
     2461 tcggcagcag taaccgcccg ttctgtggtc cgcccgtgag caaacaggag tgtagctgcc
     2521 agcgcggtcg tccttaatgc cgcttcgcac gtcaacggct ccgggcacgg tgtttccctt
     2581 ttcacattgg tcaattgcgc tgcgcggtcc aatagcccgt gcatcgcgca cttcccgcgc
     2641 ggccgctcca ttttccacga ccggaggttc cgaaaagcct gcctgtgccg gccgcaaatt
     2701 tgtcgtgaac cgtcaccgac gcatgaaccg aaccggattt cgcgacgtat cccttgaatc
     2761 ttactagatt gggatgagtc acaatgccgt cggaaacatt gacagtgaag ttgtaactgg
     2821 gatcgcatga gcccctttgc gtcacgaaga cgagatccca ggagccatcg taggcggatc
     2881 gcgcgtgtgc gacagatgcc ggggcgataa agcaggcggc tgcaatcatc cagagcgaag
     2941 gcgtcttcat tgatagttct ccgttaggcg aagcggccgc ttccaagcaa acgcaacaga
     3001 cctcgtcgat attcgaattg gggagcggcg cg
//
LOCUS       DATNOI010000526         6754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1635114,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000526 DATNOI010000000
VERSION     DATNOI010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6754
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1635114"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..233
                     /locus_tag="VN968_21275"
                     /pseudo
     CDS             102..233
                     /locus_tag="VN968_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612145.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(349..780)
                     /locus_tag="VN968_21280"
                     /pseudo
     CDS             complement(349..780)
                     /locus_tag="VN968_21280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400357.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(793..3504)
                     /locus_tag="VN968_21285"
     CDS             complement(793..3504)
                     /locus_tag="VN968_21285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL31804.1"
                     /translation="MVALIVLTVAATGALTYHNVSIVEVPRALDRLDIHARLLASDLE
                     ASVGGLRGGVLAIPRTAAVAVEGVRRATRGGGVDPFGVTTLAQWRGALSERFAAQLAA
                     NPAYHQIRLIGATDGGREIARTDRMGPENAVRVAPEAELQPRGDRDYFRRAMRLRADE
                     VDISPVELDREHGAIDTPHLPVVRASTPVVGPDGRPFGIVVIDIDLRGPFARVRGNAR
                     PGGAVYIVNSNGDYLVHPDPAREFGFQLGTPSRLQDDFPTLGGMLARGKAAPQVVQEH
                     SGAEFGAALAGARLAEGPQLFVVEAVPYADLATTLTTVRNSTVAAVLVAAIGAVLLAI
                     VLARSLTRRLTQMTAAIDSFGRGEPLSLPTRSSGEIGALARAFDRMAAEVREKTVSLE
                     TEIVERRRLFASSLDLILIVDREGTVIDISPSAEAILGYRPEEMIGRKGGALLHPGDL
                     ERAREEMRAARQGRATRNFDCRYVHRDGRIVPLTLSGAWLELEQRYFFIGRDMTERIA
                     LEQELRQSQKLDAIGHLTGGVAHDFNNILTVITGTTEILADAVAHSPQLAAIARMIDD
                     AAWRGADLIQQLLAYARKQPLQPQSTDVNRLVLDTAKLLRPTLGESVEIKMTLEEDAW
                     PAMIDPSQLGTALINLAVNARDAMPGGGKLILETGNVMLDASFATIHAEEVRLRPYVM
                     IAVTDTGVGIPADIRDRVFEPFFTTKEVGQGTGLGLSMVYGFVKQSDGHIKIDSEEGH
                     GTTFKLYLPRSSESVAPIERPVAAAEGGEETILVVEDDALVRQYVVAQLESLRYAVLA
                     AASAREALDMIAAGRAFDLLFTDVILGSGFNGPALADEILRLRPGVKVLYTSGYTKDA
                     MATHGRIDPGVALIHKPYRKIELARKIRAVLGTPSGTATQDWLRRLR"
     gene            complement(3800..3982)
                     /locus_tag="VN968_21290"
     CDS             complement(3800..3982)
                     /locus_tag="VN968_21290"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXL31805.1"
                     /translation="MTGHTPMSKVSRLEMNSSGARRRWTLEEKHRIVAESCAGSRLVS
                     ATARRYGLSASQLFRC"
     gene            4123..4370
                     /locus_tag="VN968_21295"
                     /pseudo
     CDS             4123..4370
                     /locus_tag="VN968_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044969.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5385..6101
                     /locus_tag="VN968_21300"
     CDS             5385..6101
                     /locus_tag="VN968_21300"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HXL31806.1"
                     /translation="MPLEIRQLIREMGIANPLWGAPRIHGELLKLGIDIGQTSVAKYM
                     AKRRGPPSQGWKTFLNNHADGIAAMDLFVVPTISFRLLYGLLIMGHGRRQILLFGVTS
                     HPTAEWISNQLTEACGWEQAPRYLIRDRDGAYGEVFIRRLRSMGIRDRPTSPRSPWQN
                     GFAERLIGSIRRECLDHVVVFNERHLRHVLLSYMNYYNETRTHLSLDKDAPLSRTVKR
                     AGRILCRPVLGGLHHQYIRI"
BASE COUNT         1309 a         2120 c         2066 g         1259 t
ORIGIN      
        1 gccgcacagg tcgcgatcac gcggcggcat cagccctaaa ggggcgctgg gcggaccgcg
       61 atcacggcgc agcggaatac tcatgatgct ggtggcattc ccgtgttgac gaccgacgcg
      121 tcctgaacgg tatcttttga atcttgcgct caggtgctcc gtggcacgat ctgccggaag
      181 cctctggtcc ccgcaccacc tgctataatc gctgcgttcg ttggaggcag gctagctcga
      241 ttgtcctcat ctaaattcaa gatggttgca tgcgatcaac agcggtggcg ccccagcgtg
      301 gatgaaactg ataaccatcg ctcgccgatc ctactatgaa tgggtatcga gcagcagcac
      361 gagcggggtc agcagcgccg tgatcgcgaa gaagatgaag agcgcgagca tcgcacgaat
      421 gcgcagcatt ggttcttcgg cgaacaacgt gaacacgctg agaatcagtc tggcgcagga
      481 aacccgcgca cgtggctggc tctgtcgcgg caggcttcga aacagcagcg cggcgacaac
      541 caacgttgcg cccgccgaga ccagataaac ggaacgccag ccgaacaagt ccgagagcgt
      601 gcccgccact gttcgggcta ggaggatgcc cgtgatgatg ccgctggtga cgacgccgac
      661 cccacggcca cgctcgcctg gtttggcgag gctggccgcg taggcgacca tcacttgcgt
      721 gacaacggcg agcccgccga ttgcggccat cgcgcccaat agagccgctg cggtcggcgc
      781 gagagcaacc gctcatcgca gccttctgag ccaatcttgc gtcgccgtgc cggaaggtgt
      841 gccgagcacg gcgcggatct tgcgcgcgag ttcgatcttg cgatagggct tatggatcag
      901 cgcgacgccg ggatcaatcc ggccgtgggt cgccatcgcg tccttcgtgt atccggatgt
      961 atacagcacc ttgacacccg ggcgcagccg caggatctca tcggcgagcg ctggaccgtt
     1021 gaatccgctg ccgaggatca catcggtgaa cagcaggtcg aatgcgcggc cggcggcgat
     1081 catgtcgaga gcctcgcgcg ccgaggcagc ggcgagcacc gcatagcgca ggctttccaa
     1141 ctgtgcgaca acgtattgac ggaccaatgc gtcgtcctcg accaccagaa tggtctcctc
     1201 gccgccctca gcggcggcga ccggccgctc gatcggtgcg acggactcgc tgctgcgcgg
     1261 caggtacagc ttaaaagtcg tgccgtggcc ttcctcgctg tcaattttga tgtggccgtc
     1321 cgattgcttg acaaaaccgt agaccatgct gagccctagt ccggttcctt gtccgacctc
     1381 cttggtcgtg aagaacggtt cgaacaccct atcgcgaatg tctgctggga ttccgactcc
     1441 ggtgtcggtc accgcgatca tcacataggg gcggagccgg acctcctccg catggatcgt
     1501 ggcaaaagac gcatcgagca tcacgtttcc ggtctccagg atgagcttgc cgccacccgg
     1561 catggcatcg cgggcgttga ccgccaagtt gatcagcgcg gtgccgagct gtgacggatc
     1621 gatcatcgcc ggccaggcgt cttcctcgag cgtcatcttg atctcgacgc tctcgccgag
     1681 agttggacga agcagcttgg cggtgtcgag gaccaaccgg ttcacgtcgg tcgactgcgg
     1741 ctgcagcggc tgcttgcggg cgtaggcgag caactgctga atgaggtcgg cgccacgcca
     1801 tgctgcgtcg tcgatcatcc gggcaatcgc cgcaagctgc ggcgaatgag cgaccgcgtc
     1861 ggcgaggatc tcggtggtcc cggtgatgac ggtaaggatg ttgttgaagt cgtgagcgac
     1921 accgccggtg aggtgaccga tcgcatcgag cttctgggac tggcgcagct cttgctccaa
     1981 cgcgatgcgt tccgtcatgt cgcggccgat gaagaaatag cgttgctcca gctcgagcca
     2041 cgccccgctc aaggtgagcg gcacgatccg gccgtcacgg tgcacatagc ggcagtcgaa
     2101 gtttcgcgtg gcgcggcctt ggcgcgccgc ccgcatttcc tcgcgggccc gctcaaggtc
     2161 gccaggatgc aggagcgcgc cacctttgcg accgatcatc tcctccggac gataaccgag
     2221 gatcgcctcg gcgctcgggc tgatgtcgat gacggtgccc tcgcggtcga cgatcaggat
     2281 gaggtcgagc gagctcgcaa acagccgccg acgctcgacg atctcggtct ccagcgacac
     2341 cgttttttca cgcacctcgg cagccatacg atcaaaggcg cgggccagtg cgccgatctc
     2401 gccgctggac cgggtcggca ggctcagcgg ctcgccgcgc ccgaagctgt cgatcgccgc
     2461 cgtcatctgg gtgagacgcc gggtcaagga gcgcgccagc acgatggcca gcaggacggc
     2521 gccgatcgct gccaccagga ccgccgccac ggtggagttg cgcacggtgg tcaacgtcgt
     2581 ggcgagatcc gcgtacggca ccgcctcgac gacgaatagc tggggtcctt cggcaagccg
     2641 cgcaccggcc aaggccgcgc cgaactccgc accgctatgc tcctgaacca cctgcggcgc
     2701 cgccttgcca cgagccagca tgccgcccag cgtcggaaaa tcgtcctgca ggcgcgacgg
     2761 cgtgccgagc tggaagccga actcgcgagc cggatcggga tggaccagat agtcgccgtt
     2821 gctgttgacg atgtaaactg cgccgccggg tcgggcgttc ccgcgcacac gcgcgaatgg
     2881 cccacgcagg tcgatatcta tgaccacaat cccgaatggc cgcccgtccg gccccacgac
     2941 cggagtcgac gccctaacga ccggcaaatg tggcgtgtcg atcgctccgt gctcccgatc
     3001 gagctcgacc ggtgagatat cgacctcgtc ggcgcgcagc cgcatggcgc gccggaagta
     3061 atcacggtcg cccctgggct ggagctcggc ttcgggcgcg acccggacgg cgttctctgg
     3121 ccccatgcgg tcggtccgcg cgatctcccg gccaccgtcg gtggcaccga tcagacggat
     3181 ctgatggtaa gccggattgg cggccagttg ggcggcgaac ctctcgctca gcgcgccgcg
     3241 ccattgggcc agcgtcgtga cgccgaaggg atctacgcca ccgcctcgcg tcgcgcgcct
     3301 gacgccttcg acggcgacgg cagccgttct cggaatggcg agcacaccac ctcgcaatcc
     3361 gcccacagag gcctcgagat cggaggcaag cagacgggca tggatgtcga ggcgatcaag
     3421 cgcgcgcgga acctcgacta tggacacatt atgataggtg agcgcgccgg tggcggccac
     3481 ggtgagcacg atcagggcga ccatccccgc cgtcaaacgc gccgacaacc ccatgcccgg
     3541 ctccgtcctg gcgctccgcg gctcattcag agccggctgc agatcagtac cggttggacc
     3601 cgccgggagc aacgcgacga ctggttccga ccacgcccgc aatcgacggc gcgagcgatc
     3661 aggccgggcg acgccactac gtaaacgtgt agcagcttcc cgccacctgg tcaaaaccgc
     3721 cagtcagcta tcggggagcc tccatccagc cgcagggcgc ccgccacgcg tcgcgcggac
     3781 ggcgaatcaa gttgcccggt cagcatctaa aaagctggct cgcagataac ccgtaccgac
     3841 gcgctgttgc cgacactaac cgcgaacccg cgcaactctc cgcgacaatc cggtgcttct
     3901 cttccagcgt ccagcgtcgc cgggcacccg acgaattcat ctcaaggcgg ctaaccttcg
     3961 acataggcgt atgaccagtc atacgcctat gtcttcacag ccgtttcgtc tcacgcaagg
     4021 cggccttcgg tggaggaata ccgctcatcc cgcccatcgg ctggatgagt tgctaccctg
     4081 gaattggacg cctccggcgt cagcgatctc ggtccgagcg gcgtgaccac gcacgtcaac
     4141 aaggtccatc acgtcaccac tatcacccgc gttgccgagc agctcggcaa agacgaggat
     4201 ttgctgcggg acgtcgccag cgagaaatcg aggacggcgt catctgggtc tacggcgtcg
     4261 gagaagacgg cctcctagcg ttcaccgact tcggcattga gagcctgatc gaactcatca
     4321 agatgtacaa agacgatcct acgctgctca agcgttacga tccgcaataa tgaccaccat
     4381 gcggcctacg ccggatgctt acatggcaag cgcgcgcggc tcggcaacgc gagaggcgac
     4441 ccttcgctgt ggcgagccga tgcaacttcg gcgagccgat gcaacttcaa ttgtgggtaa
     4501 ggattttttg cctgcaatcg cgccgaagcg cccgttgtcg ggtagggcca gcggcgtttt
     4561 acaaatggcg cgatgatgca atagtgccgc tgatttgccc gacgtgtcaa aatgttttcg
     4621 cggggatcgc tcaaggcatc gatgccagcg accaccatgc tactttgcat ggggttgttt
     4681 tcgatatttt ggttggaagc cagagccgcg tcggcccttg cggctgcgtg gctgcgccac
     4741 gcgtagccct ttcgtggaaa tagcctccgc aagttccagt ccgtcttcgc ttccgcttcg
     4801 ctccagctac gccggacacg cttcaccctt cggtctcagc gtggcagcgc cacgcgtagc
     4861 ccgaagggcg aagcgtggtg ggcccggcag cagccataat accggggcaa accggagaaa
     4921 aatcaatcat gtagtctgat gttgatcaga aacatgccaa caaagatgcc aacattcttg
     4981 gcttgtccct ctcaaggctg aaacaggggt tcgattcccc ttgtccctgt cggaaaccca
     5041 atctggctgg cggttgaccg agaatcggat gatgctgttg tgtcatgaga gatctggtca
     5101 ggctcattgt ttggatggtc gtcgatctga tgcggtcgta ggcgacgctt gaggcagaac
     5161 tattgacgtt gcgacagcag ataaacgttc tgcggcgaac cgcccctaag aaacaatcct
     5221 tcagcgtcat cgaccgactg gtattcgttt gtctgtatag gttgttccct aaagtatgcg
     5281 atgcgttagc gatcgtgaag ccggacacca tagtccgttg gcaccgcgcc ggattcagat
     5341 cgtactggcg ctggaagtcg cgacctcgtg gtggtcggcc gaccatgccg ctggaaatac
     5401 gccagctcat ccgggagatg ggcatcgcaa atccactgtg gggcgcgccg cgcatccacg
     5461 gcgagcttct caagctcggc atcgatatcg gtcagacgag cgtagccaag tatatggcca
     5521 agcgtagagg gccgccgtcc cagggttgga aaacatttct caacaatcat gccgacggca
     5581 tcgcggcgat ggatctgttc gtcgttccga caatctcgtt tcgtttgctc tacggtctac
     5641 tgatcatggg gcacggccgg cggcagattc tattgtttgg cgttacatcg catccgaccg
     5701 cagaatggat ctcaaatcag ctcacggaag catgcggctg ggaacaagcg ccccgctatc
     5761 tgatccgcga tcgggatggg gcttatggcg aggtcttcat ccgaaggctt cgatcaatgg
     5821 gaattcgcga tcgaccgaca tcgccacgtt ccccatggca aaacggattt gctgaacgac
     5881 ttatcgggtc gatccgacgg gagtgccttg accacgttgt tgtgttcaac gagcggcatc
     5941 tccgtcacgt gctgctgtcc tatatgaatt actataatga gacgcgcacg cacctgtcct
     6001 tggataagga tgcgccccta tcgcgtactg ttaagagggc agggcgtatt ctttgccgcc
     6061 cagtcctcgg cgggctgcat caccaatata tccggatttg atttacgaca ggcacaggga
     6121 gcttccagtt tcgcaatttc cgcggggcgc ttaccttcct tccaaatatc tgcgaattaa
     6181 ctcgcgtttg agatccctca ggcgcatact cagccaatca ccgctccgcc tcctaggcat
     6241 taccggtgac agagagtgtt gcgctcccgc ctgttcgagg gaagcatcat gccccaatct
     6301 caacacctgt cgtccgtcat tccattcgtt gcaattgagc gcccggcctg tccgaagtgc
     6361 aaggcccaga tgatgcttgt tagcatcgag ccagcacgcg cccggggcgt cgacttgcaa
     6421 acgtttgaat gcgctgtctg caatcaggta cttacgagct ttgccgcgta tgaagacccc
     6481 atgaagtcca agggtcttgg acgctggctt caaggcgatt tgcacccacc gaagtagggc
     6541 ccgccggcgt gaaacggcga gctactgtcc ggcgagttgg tcgggcatct aaacgacgcg
     6601 cacggcaccg attcactgac agattgttcc ccggcagagg cagatctcgc caaatctgac
     6661 tgctgaaggc gcagggaaaa tgatcccgat caaatcgacc ggtcgaacgc taaccgagat
     6721 gcaagaactg gaggcgatgg ccgcggagct gcta
//
LOCUS       DATNOI010000527        18207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_803215,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000527 DATNOI010000000
VERSION     DATNOI010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18207
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803215"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(687..932)
                     /locus_tag="VN968_21305"
     CDS             complement(687..932)
                     /locus_tag="VN968_21305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31807.1"
                     /translation="MPFPCFKIKIEGKFCIESWDRQQIREANSEECANLQFRSNHSAI
                     VFENALKAYFGLRPWEPRFDSLKAEAVAKVSTMFLHN"
     gene            complement(945..1181)
                     /locus_tag="VN968_21310"
     CDS             complement(945..1181)
                     /locus_tag="VN968_21310"
                     /inference="COORDINATES: protein motif:HMM:NF026766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm26 family immunity protein"
                     /protein_id="HXL31808.1"
                     /translation="MNIAVNVGLVFAVPLADKMFGFGQLIAWQRPIFYVAGYDKRANS
                     PDVDEGDIRKAKSILLGNFFDILIRNGRWQAIRQ"
     gene            complement(1356..2585)
                     /locus_tag="VN968_21315"
     CDS             complement(1356..2585)
                     /locus_tag="VN968_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXL31809.1"
                     /translation="MHRPVDASGTTTPHAQAARLPLSRFKVIDLTLARAGPSCVRTLA
                     DWGADVIRVEPPPGDDEVGELVGRRDGSDFQNLHRNKRAITLNLKTDEGREILMRLAE
                     AADVVVENMRPGVTRRLGVDFESVRKRNPRIVYGSISGFGQYGPYAERPSIDQIAQGM
                     SGIMSVTGIPGQGPLRVGVAVTDIMAGAFLAQGILIALLDREVSGEGRWVQTSLIEAG
                     ITLLDFQATRWTMDKKVPPQEGNNHPTNTPMGLFATADGYLNLAATSNKNFQKFCKLI
                     DRDKMATDPRFASSALRGRNREALNELIAFALRAKSTREWFELLVEAGLPCGPVYSIK
                     DTFADPQVEALRIKRSVAHPRLGEIDLVAQPCEITGFDREIRTATPDLGEHNDDVLGS
                     LGYGADEIEKLKAAGVI"
     gene            complement(2578..3654)
                     /locus_tag="VN968_21320"
     CDS             complement(2578..3654)
                     /locus_tag="VN968_21320"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31810.1"
                     /translation="MDKLQLTGLTKKFGGLAVVDNLNLSLRSGEFVSLLGPSGCGKTT
                     TLRMIAGFIAPTAGTVEMDGKEMSSASAWLPPERRRMSMIFQSYALWPNMTVEQNVAF
                     GLKMRKVAKDELKRRVGQILDVVQLRHLAARYPNELSGGQQQRVSLARALVVQPEILL
                     LDEPLSNLDANLREEMRGEIRRLHNEFGITSIYVTHDQSEAMTTSDRIAVMNQGRIEQ
                     IDDPLTLYTRPKTRYVAEFIGRSNILQGKPNGIRMTFDGFEVASARLGKRGPIRTREF
                     SLRSQNVAVHAKCPVGDRVILPGSIIERAFLGETWDYAFQSDTSGLKLRVMAPPLSVF
                     AVGQKVWLEINPSHIIPIQDDQHA"
     gene            complement(3654..5399)
                     /locus_tag="VN968_21325"
     CDS             complement(3654..5399)
                     /locus_tag="VN968_21325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXL31811.1"
                     /translation="MVELRIESDEDAAAAPVRWIAAIRNAMNPTTLVALLLASILIFL
                     VANPLFQLVKDSFTKASDHSFTLANYVTAFGRPRYVQALINSMELGVAAAAFASLIAV
                     PLAWGVSRTDMPGRNFVHVMVLASFLIPPYVGAIGWILLGGPNAGWINKAWMAVTGAP
                     AGPFNIFTFWGLALVTALYSFPLIYVFTKSALDLMSTEMEEAASILGAGPAQTTLRIT
                     LPLALPAILGSVFLVFLEVLGLYGTPALIAIPAGFNVVTTQLAAFFENPVRVEVAAAF
                     SMPMVGISIALLWVQRRLLARKSYVTVGGKGGHREPMALGPFRWVLFAYAMAITGFSV
                     FMPLYIILQTSFSKAWALPMSSANFTFHNFREVLFDQLTVRQALFNTFLYAIVTATIC
                     TFLGFAVAYISQRRLLPFSQALASITLAPFAVPGIVLAVCFYAAYAPPPFALYGFGAL
                     VVIAFVTRFLPISFTNSNSAIQGLHPELEEAVRIAGGNQARALWLVVLPILKKSLFGS
                     WLLIFIIGTRELSTAMFLSGPQTRVISVLTLELSEQGQYEMLSAISVLLLTVTGVVTL
                     IGTLILGRDFMLRRT"
     gene            complement(5527..6348)
                     /locus_tag="VN968_21330"
     CDS             complement(5527..6348)
                     /locus_tag="VN968_21330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXL31812.1"
                     /translation="MLDVPKSTEKSYADGKILQNIADGVGVITFNNAEKRNAMSLDMW
                     EGLGHALVELRDDPDIRVVILVGAGDKAFVSGADISQFEKTRHNAEASEEYSKKSAAQ
                     RALLADYPKPIIACIRGFCLGGGMQVAMAADIRIASENSQFGIPAAKLGIAYGYDGLK
                     HLVSLVGPSWARLLMYTGMRIDSAEALRIGLVDRVVPDADLWNATSEIARTISCNAPL
                     AIKAAKITIAQVLKDPDKRDMDAIKQIGTDCMDSEDFREGRRAFMEKRKPQFHGR"
     gene            complement(6387..7112)
                     /locus_tag="VN968_21335"
     CDS             complement(6387..7112)
                     /locus_tag="VN968_21335"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXL31813.1"
                     /translation="MMILHHGWRSSASRRVRLCLEEKRLSYDGHVVDMARLEHHSPEY
                     LKINPNGVIPTLIHDGRPLHESGTICEYLDETFADPPLRPDTPYERALMRNWIRHIDG
                     LIHNLIIFNWRHHLQKVASQWSDEELAEKLKNIPSKERQASWLRVARKPYTEEERDAA
                     RAKLVQLPDKMEEALQPSGWLVGKAYSIADIAAVPFAKRIDEEIAPDEMAAKKHPRVA
                     EWWTKIQARPAFARANFGPFLES"
     gene            complement(7143..8132)
                     /locus_tag="VN968_21340"
     CDS             complement(7143..8132)
                     /locus_tag="VN968_21340"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXL31814.1"
                     /translation="MSLRPARAQSDYIAELYAKARLEGELTWYIAYWPSDRSEKHSAI
                     FTKTFPGVKVNVVRSTAQVAYQRLSQDIQAGAANCDVFATTDMGQYVSLKDRGLLMPY
                     KMQSIDAIDARFRGVDPDDAYQIVNATTVSLAYNTNKVKPEQAPKSWKEFIDPKWKSQ
                     SVVGHPGFSGFVGTWVVQMNKLYGWDYFEQLAAQKPHVGRSIIDTVTVLVSGERSVGA
                     SPTALVLRNAAQGNPIAVSYPEEGSVLMISGSAIMKDTRHPNAAKLFMEFCYSAQNAI
                     EDVEEFGLPLRRDAVLPAGYKKLDDLKTIRPSIGEIIKGIPELTEKWRDTFGV"
     gene            complement(8326..9651)
                     /locus_tag="VN968_21345"
     CDS             complement(8326..9651)
                     /locus_tag="VN968_21345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXL31815.1"
                     /translation="MRRMTLIAIATILATASAFAQGAGNAGTDMADSGAAPHPAKPVK
                     PAKPAAASTPESRSAAALALSHEPTFDEGTAQRIREAALSYSDLAVRGGWPAIPADAK
                     FASGTQGPHDDLLRQRLLISGDLDPDKASGAYDDVLAEGVKRFQARHGLAPTGTVTPR
                     TLAALNVPVQKRIKQLEASLERLENMNFAFGQRYVVVNLPAAFAEAVENDHVVRRYRV
                     IVGKTEKPSPTLTAEITSVNLNPTWTVPSSIAKTEISAHMRRDPGYLARMHMEVLDAH
                     EAPIDPRSVDWSGAHTPNFTVRQQNGGWNALGAVKIDMPNPYSVYMHDTNQRNLFSDD
                     YRFDSHGCSRVDNVRDLAAWLLSEEMPKWNRAAIDAAIATGQREDIVLPKKVPVAWIY
                     LTGWMTKDQTVQFRNDIYDQDEQLLEATAEEAAFFSQAANHPLTAHLAQ"
     gene            10105..10980
                     /locus_tag="VN968_21350"
     CDS             10105..10980
                     /locus_tag="VN968_21350"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL31816.1"
                     /translation="MIVPYPAGGTTDFLGRLVADQLKTGLGATVIVENKPGAGTTLGA
                     DQVARAEPDGYALLMATSTTLAINRTLYKKLPYDPVKDFTPIALVAGVPFALIVNPQV
                     PAKTLAEFIAYAKSTPGLAYGSAGNGSPQHLGAEMLKEAAAIDIRHVPYRGSVPAMLD
                     VIAGHIPFMVVDLQPALPQIREGKVRVLGVTTPRRVAIASDIPTLAEAGLPGFELVAW
                     QGVVAPAGVPRAIVDPLAAQIAKLMADPATREKLTTIALEPLAGSTPDGFAAFIKTEV
                     DRWGDIVKNSGAEPE"
     gene            11027..12718
                     /locus_tag="VN968_21355"
     CDS             11027..12718
                     /locus_tag="VN968_21355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL31817.1"
                     /translation="MPEMTEPDVLIVGAGPVGLTLAIDLAWRGIEVTVVETRGRAAPP
                     EPKCNHVAARTMEIFRRLGLSEKVRNAGLPADYPHDVAYRTTFTGPELARIPIPCRRD
                     RFTRTDGPDCNWPTPEPPHRINQIFLEPILFEHAAAQPRIRIINRTSVEDVVVEDTSA
                     HVTLRDLDTGAVRRLSCRYLVGCDGARSVVRKAIGAELAGDAIVQRVQSTFIRAPGLI
                     DRQQHARAWGTGVINPRRSGMVYAIDGRERWLVHNYLKPGEIDFEQVDRDGCIRTILG
                     VGADFDYDIISREDWYRRRLIASRFRHACAFIAGDAAHIWVPYAGYGMNAGIADAMNL
                     SWLLAAHLNGWAPASILDAYEAERWPITSQVSRFAMSHAEAEIRRRGAVPDEIEEAGP
                     RGEHARREVGRLAYEINVQQYACAGLNFGTYYDRSPIIAYDGAEHPAYTMNSYTPSSV
                     PGCRTPHLWRGDGGSLYDAMGPEFTLLRFDPALDVVALEAAARNRGVPLKILDIEPPC
                     TAIFHGGGLVLSRPDQHVAWRGDKSPADPPGLIDRIRGAANQEFARAKDRPTRRT"
     gene            complement(12715..13110)
                     /locus_tag="VN968_21360"
     CDS             complement(12715..13110)
                     /locus_tag="VN968_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL31818.1"
                     /translation="MKQAAAKKMKQRSAGKPDIELGKRIRLRRVEQKISQAELGDKLG
                     VSFQQVQKYEKGVNRVGAARLQQIATALDVPVTFFYDGDGKAREVESLLFLDSAFSLR
                     LLRAYSKIKDQTVQRQLVSLMESIAANED"
     gene            complement(13118..14164)
                     /locus_tag="VN968_21365"
     CDS             complement(13118..14164)
                     /locus_tag="VN968_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL31819.1"
                     /translation="MRLVLERGVPQEERHKLAIGSLTDWDAARVFLEVVRCGSFRSAA
                     ERLDLSINVVRRRIDEFERQIGATLFTRDVHGTRLTDEGSLVVSAVERMEAASFDLLR
                     ASKSVTHTLSGEVRVAVTEGLGTFWLAPRLVEFQQSFPNILVDLHCAMRSADVSRHEA
                     DVAIHLSRPAAPDVKLVRLGRMHLMFFASQKYIDTYGVPRTVDDFAKHRLVMQVADQT
                     AAKETFESVFPGYSQQELLVMKTNVSSANYWAVAYGAGIGVFPSYACELGGKIIPLEI
                     ELRRPFDIWLSYHPGSGRIPRVRRMIDWLVEAFNPAKFPWFKDEFVHPSEFKAVYKGE
                     SLTHLFGGFSTEGR"
     gene            14341..14931
                     /locus_tag="VN968_21370"
     CDS             14341..14931
                     /locus_tag="VN968_21370"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HXL31820.1"
                     /translation="MSSIVSLRTFANSSNVPIVVLADMQQEYPAKPRPLSISKINRAL
                     DNCRRVLDHARDIGLPVAFMRQLNESAFFNRATPFVRWIEGFEPYRNEMVFERSNPSC
                     YSCEPFAALMNQSRGGIVLAGFAGESACLSTLIDAFHRNHRVTYSCDASASHALEDVP
                     ANEIHRAVSKISGLYGEVYETTDWIASTVPRKLGVG"
     gene            14960..15208
                     /locus_tag="VN968_21375"
     CDS             14960..15208
                     /locus_tag="VN968_21375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31821.1"
                     /translation="MHSVLAALEQCRAVLTDSADRETAQLLSVAILQLRMKLNQVSDQ
                     DLKALCDAMVPDEDPAEATQDPKSSQNERRRASLKLVK"
     gene            complement(15500..16819)
                     /locus_tag="VN968_21380"
     CDS             complement(15500..16819)
                     /locus_tag="VN968_21380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HXL31822.1"
                     /translation="MSTISATAVSVRKPLYQSLFVQVLVALLLGIVLGMAVPDFAVEL
                     KILSDAFLKLISMIVAPIVFCVVVHGIAGAGDLKKVGRVGVKALIYFEVMTTVALVVG
                     LLLAYLFGPGHGMNIDTSTLDPKALSSYADNAHKLQGGGIGSFLMNIIPTTSFDALSR
                     NDVLQVLFFAIIFGVSLALVGGEKAERIGSLIDAVSTVLFRAMGLIVRLAPLGGLGAV
                     GYTVGKYGVGSLKQLISLVALFYVSLAVFVLGVLGSVMALVGLNIFKFLGYLREELTI
                     VLATASSDAVLPQIMRKLERLGVKDSVVGLVIPTGYSFNLDAFSIYLTLAVVFIAQAT
                     NTPLSFGDLMLVLGVSLVTSKGAHGVPGSAIVILAATLNAVPSIPAIGLVLVLSVDWF
                     IGMARALGNLIGNCVATVVVAGWEGDLDRAKAHRVLHGDELVDVTAG"
     gene            17036..17770
                     /locus_tag="VN968_21385"
     CDS             17036..17770
                     /locus_tag="VN968_21385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HXL31823.1"
                     /translation="MIHDVSIPAALIAGLVSFLSPCVLPLVPPYLVYLTGATIEHVVN
                     DETVSSSKRAVMGAAAMFVLGFSTVFVALGASASLIGGLIRAWSGELSIVAGIVIIIM
                     GLHFLGLTRIGFLMREGRLPIPKPVGLWGAYVMGLAFAFGWTPCIGPILAAILSVAAA
                     EATVTKGAGLLAVYSAGLGIPFLLAAFMVEQFSSLFSRMKRHLAAVEHAMGVLMVITG
                     IGFLTGAASNVSIWLLETFPGLQNFG"
BASE COUNT         3413 a         5584 c         5644 g         3566 t
ORIGIN      
        1 ctgcacccgc agcctcgcgt gcacttggta gtggtagaac gcacacgagt aacaacgagt
       61 acaccggaac gcccggcatt cccgcacgca atggttttaa cggtttcctt cgtgctctcc
      121 ccggtgaccg ggctcttttg ccaccgtcgc ttgcggatta aggttttgtc cgcgcccggt
      181 agggccgact tgccatccgc aaacttgacg ccagcgtcgg ggcgtcagga ccacacgact
      241 tcgccgtacg ctgcaacatc tttcgtccgc tcgcttggtg atcgctcaca ggcctcatcg
      301 acctgccctg cgatcccatc gcgcgcaaaa cgctgccgcg tccaccgcat ccctccccgc
      361 gttcgtgacg atggccaacg cccctcttgt ggggtgggat ggcacgaatt tgtagagatg
      421 atttgcccga cgggtgaagc gaaatatttt tgcaaacagg gctggacagc cctgtcgaaa
      481 aaccgcccga cgggcaaatc accacgcggc atcgagagca aattccgctc gcgtaccgca
      541 cgcggtgcag cgtactccca gcgatgccaa cgggtcgcgc gaatgcgcgc tcgatgacag
      601 gctccgcatc gtccggtcac gctgctctga gaaaacccag cgcaccttgt caccgtattt
      661 tgtaagtccc cgcgggagat tgcttatcag ttatgcaaaa acatcgtcga gactttagcc
      721 accgcttcgg ctttcagact gtcaaatctt ggctcccaag ggcgcaggcc aaaataggct
      781 ttcaacgcat tctcaaaaac aattgcactg tgattcgaac ggaattgtaa attagcgcat
      841 tcttcggaat tcgcttcgcg gatctgctgt ctgtcccagc tttcgataca gaacttgcct
      901 tcaatcttaa tcttaaaaca ggggaacggc actttcggcg tctcctattg tcgaattgct
      961 tgccatcgcc cattgcggat caagatatca aagaagtttc ccagcaagat ggattttgcc
     1021 ttccttatgt cgccctcgtc cacgtcaggc gaattcgcgc gcttgtcata gcctgccacg
     1081 tagaatatcg gccgctgcca tgcgatcaac tgcccgaatc cgaacatttt gtccgcaagt
     1141 gggacggcga agaccaggcc gacattgact gcgatattca tggatagttc accgtcccac
     1201 ccgcgttgca gtagttttga tcgctcattt ggtgacgtgc attttgcatt gacccaccag
     1261 agtccgtaac aagtagcccg catgagcgca gcgatatgcg ggagccaagg gaaatgagaa
     1321 aaccccggat gtcgcttcgc tcatccgggc tacaatcaaa tcacccccgc cgccttcagc
     1381 ttctcgatct cgtcggcccc ataccccagc gatcccaaca cgtcgtcatt gtgttcgccg
     1441 aggtcgggcg tggcggtgcg gatctcgcgg tcaaaaccgg tgatctcgca gggctgcgcc
     1501 accaggtcga tttcgccgag gcggggatgg gcgacggagc gcttgatgcg caacgcttcg
     1561 acctgcgggt cggcaaaagt gtccttgatg ctgtagaccg ggccgcaggg aaggccggct
     1621 tccaccagca gttcgaacca ttcgcgggtc gactttgcgc gcaaggcgaa agcgatcaat
     1681 tcgttcagcg cctccctgtt tcttccgcgc aacgcgctcg aggcaaagcg cggatcggtc
     1741 gccatcttgt cgcggtcgat cagcttgcag aatttctgaa aattcttgtt gctggtcgcc
     1801 gccagattca agtagccgtc ggcggtggca aaaagcccca tcggcgtgtt ggtcggatgg
     1861 ttgttgcctt cctgcggcgg caccttcttg tccatggtcc agcgcgtggc ctggaagtcg
     1921 agtagcgtaa tgccggcctc gatcagcgag gtctgcaccc agcggccttc gccagagacc
     1981 tcgcggtcga gcagcgcgat caggatgccc tgcgcgagga acgcgcccgc catgatatcg
     2041 gtgacggcga caccgacgcg caggggtccc tggcccggga ttcccgtcac cgacatgatg
     2101 ccggacatgc cctgcgcgat ctggtcgatc gacggccgct cggcataggg gccgtactgg
     2161 ccgaaaccgg agatgctgcc atagacgatg cgcggattgc gcttcctcac ggattcgaaa
     2221 tcgacgccga ggcgcctggt gacgccgggc cgcatgttct cgacaacgac gtcggccgct
     2281 tccgccagtc gcatcaggat ttcgcggcct tcgtcggtct tcaggttcag cgtgatggcg
     2341 cgcttgttgc gatgcaggtt ctggaaatcc gaaccgtcgc ggcggcccac cagttcgccg
     2401 acctcgtcgt ctcccggcgg cggctcgacc cggatcacgt cggcgcccca gtcggccagc
     2461 gtgcgcacgc aggacgggcc ggcgcgcgcc agggtcaggt cgatgacctt gaagcgcgac
     2521 agcggcagcc gggccgcctg cgcgtgaggg gtcgtggtgc cggaggcatc gacgggtcta
     2581 tgcatgctgg tcgtcctgga tcgggatgat gtgtgacggg ttgatttcga gccagacctt
     2641 ctggccgacg gcgaaaacgc tcagcggcgg tgccatgacg cgcagcttca gaccgctcgt
     2701 gtccgactgg aacgcgtagt cccaggtctc gccgaggaac gcgcgctcga taatgctgcc
     2761 gggcaggatc acgcggtcgc cgacggggca tttcgcgtgc accgcgacgt tctgcgagcg
     2821 cagcgagaat tcgcgggtgc ggatcggtcc gcgtttacca agccgcgcgg aagccacctc
     2881 gaatccgtca aaggtcatcc tgatgccgtt cggtttgccc tggagaatgt tcgaacgccc
     2941 gatgaattcg gcgacatagc gcgtcttcgg ccgggtgtag agcgtcaggg gatcgtcgat
     3001 ctgctcgatg cggccctggt tcatgaccgc gatgcggtcc gacgtcgtca tcgcctcgga
     3061 ctggtcgtgg gtgacataga tcgaggtgat gccgaattca ttgtgcagac ggcggatctc
     3121 gccgcgcatt tcctcgcgca gattggcatc aagattggac agcggctcgt ccagcagcag
     3181 gatttccggc tgcaccacca gcgcgcgggc cagcgacacc cgctgttgct ggccgcccga
     3241 gagttcattg gggtagcgcg cggcgaggtg gcgaagctgc accacatcga ggatctgtcc
     3301 gacccggcgt ttgagttcgt cttttgccac tttccgcatc ttcaggccga acgcgacgtt
     3361 ctgttcgacc gtcatgttcg gccacagcgc gtagctctgg aagatcatcg acatccggcg
     3421 ccgttccggc ggcagccagg cgctcgccga tgacatttct ttgccgtcca tttccaccgt
     3481 gccggcggtc ggcgcaatga aacccgcgat catccgcaac gtcgtggtct tgccgcagcc
     3541 ggacgggccc agcagcgaca cgaactcacc tgatcgcagc gatagattga ggttgtcgac
     3601 gacggcgagg ccgccgaact tcttggtgag gcccgtgagc tgcagcttgt ccatcaggtg
     3661 cgcctcagca tgaagtcgcg gccgaggatg agggtgccga tcaatgtcac gacgccggtc
     3721 acggtgagca gcagaaccga gatcgcggag agcatttcgt actggccctg ttcgctcaat
     3781 tccagcgtca gcaccgagat cacgcgggtc tgcgggcccg acaggaacat cgccgtcgac
     3841 aattcgcggg tgccgatgat gaagatcaag agccagcttc cgaacaggga ttttttgagg
     3901 atcggaagca ccaccagcca gagcgcgcgg gcctggttgc cgccggcgat ccttactgct
     3961 tcctcgagtt cgggatgcag gccctggatc gccgaattgg aattggtgaa cgaaatgggg
     4021 agaaaccgcg tgacgaaggc gatcaccacc agcgcgccaa aaccgtacag cgcgaacggc
     4081 ggcggcgcat aggcggcata gaagcagacc gccagcacga tgccgggcac cgcgaacggc
     4141 gccagcgtga tgctggccag cgcttgcgag aacggcaaca gcctgcgctg cgagatatag
     4201 gcgaccgcga agccgagaaa cgtgcagatg gtggcggtga cgatggcgta caggaaggtg
     4261 ttgaacagcg cctgccgcac cgtcagctgg tcgaacagca cctcacggaa attgtgaaac
     4321 gtgaaattcg ccgatgacat cggcagcgcc caggccttgc tgaacgacgt ctgcagaatg
     4381 atgtagagcg gcatgaacac cgaaaagccg gtaatcgcca tcgcataggc gaacagcacc
     4441 cagcggaacg ggccgagcgc catcggttcg cgatgcccgc ccttgccgcc gacggtgaca
     4501 tagctcttgc gcgccaatag acggcgctgc acccacaaca gcgcgatgct gatgccgacc
     4561 atcggcatcg agaaggcggc ggcgacctcg accctgacag gattttcgaa gaatgccgcc
     4621 aactgcgtgg tcacgacgtt gaagccggcc gggatcgcga tcagcgccgg cgtgccgtag
     4681 aggccgagca cttcgaggaa caccagaaag accgagccga ggatcgcggg cagggcgagc
     4741 ggcagggtga tgcgcagcgt ggtctgcgcc ggcccggcgc cgaggatcga ggcggcttcc
     4801 tccatctcgg tcgacatcaa atcaagcgcg gatttcgtga acacgtagat cagcggaaac
     4861 gaatacagcg ccgtgaccag cgccagcccc cagaaggtga aaatgttgaa cggtccggcg
     4921 ggcgcgccag tgaccgccat ccaggccttg ttgatccagc cggcattggg gccgccgagc
     4981 agaatccagc cgatcgcgcc gacatatggc gggatcagga acgacgccag caccatgaca
     5041 tggacgaaat tgcgcccggg catgtcggtg cgcgaaacgc cccacgccag cggcaccgcg
     5101 atcagactgg cgaaggccgc ggcggcaaca ccgagctcca tcgagttgat cagcgcctga
     5161 acgtagcgcg gccggccgaa cgcggtgacg tagttggcga gggtaaagct gtgatcggag
     5221 gccttggtaa aactgtcctt gaccagctga aacagcgggt tggccaccag gaagatcagg
     5281 atagaagcga gcagcagcgc caccagtgtc gtcggattca tggcgttgcg gatcgccgcg
     5341 atccatcgca ccggcgccgc cgcggcatct tcgtcgcttt ctatcctaag ttcgaccatg
     5401 agatcatgat ccgattggat tgaatcggat catgatctca tctctttgtc tgagcatgat
     5461 ctccgcgcaa acgcgttccg cgtttgtcgc gagggaaaac cggtttccac ttttccggat
     5521 catgctctat cgcccgtgaa actgcggctt acgtttttcc atgaaggcgc ggcggccctc
     5581 gcggaaatcc tcagagtcca tgcagtcggt gccgatctgc ttgatcgcgt ccatgtcgcg
     5641 cttgtcgggg tctttcagca cctgcgcgat ggtgatcttg gcagccttga tggcgagcgg
     5701 cgcgttgcag gaaatggtgc gggcgatctc ggacgtcgcg ttccacaggt cggcgtccgg
     5761 caccacccga tcgacaagcc cgatccttaa cgcttccgcg gaatcgatcc gcatgcccgt
     5821 atacatcaga agccgcgccc atgacggccc gaccagcgac accagatgct tgaggccgtc
     5881 atagccatag gcgatgccga gtttcgcggc gggaatgccg aactggctgt tctcggacgc
     5941 gatgcggata tcggcggcca tcgcgacctg catgccgccg ccaagacaaa agccgcggat
     6001 gcaggcgatg atcggcttcg gataatccgc cagcagcgcg cgctgcgcgg cgctcttctt
     6061 ggaatattct tccgaggcct cggcgttgtg gcgggttttc tcgaactggc tgatatcggc
     6121 gcccgagacg aacgccttgt cgccggcgcc gaccaggatc acgacgcgga tgtcagggtc
     6181 gtcgcgcaat tcgaccagcg cgtgaccgag cccctcccac atgtcgagcg acatcgcgtt
     6241 gcgcttttcg gcattgttga aggtgatgac accgacgccg tcggcgatgt tttgcaggat
     6301 cttgccgtcg gcgtaggatt tctcagtgct tttggggaca tctagcatcg gtgcggcttt
     6361 cgggtatgag aggtgcggtc tgcggctcag gactcgagga agggaccgaa attcgcccgc
     6421 gcgaaggccg gtcgcgcctg tattttagtc caccattcgg cgacgcgggg atgtttcttc
     6481 gccgccatct catccggcgc gatttcctcg tcgatccgct tggcgaatgg aactgctgcg
     6541 atatcggcga tcgaataggc cttgccgacc agccagcccg acggttgcag cgcctcttcc
     6601 atcttgtccg gcaactgcac caatttggcc cgcgccgcgt cgcgctcctc ctcggtatag
     6661 ggcttgcggg ccacccgcag ccaggatgcc tggcgttcct tgctcggaat gttcttcagc
     6721 ttttcggcca gttcctcgtc cgaccattgc gacgccactt tctgcagatg gtgccgccag
     6781 ttgaagatga tgaggttgtg gatcagtccg tcgatgtgcc ggatccagtt gcgcatcagc
     6841 gcgcgctcat agggcgtatc cgggcgcagc ggcgggtccg caaaggtctc gtcgagatat
     6901 tcgcagatcg tgccactctc gtgcagcggc cttccgtcat ggatcaatgt cgggatcaca
     6961 ccgtttggat tgatcttgag atattccggc gagtgatgct cgagcctggc catgtcgacg
     7021 acgtggccgt cgtagctgag gcgcttttcc tcgagacaca gccgcacgcg gcgtgatgcg
     7081 ctggagcgcc agccgtggtg aaggatcatc atggtctgct ccactcgaag ctcattgccg
     7141 gcctacacac caaacgtgtc gcgccatttt tccgtcagct cgggaatgcc cttgatgatc
     7201 tcgccgatcg aggggcggat cgtcttgagg tcgtcgagct tcttgtagcc cgcgggcaac
     7261 acggcatcgc gccgcaacgg cagcccgaat tcctcgacgt cctcgatggc gttttgcgcc
     7321 gaatagcaga attccatgaa cagttttgcg gcgttgggat gcctggtgtc cttcatgatc
     7381 gccgaacctg atatcatcag gaccgagccc tcctcggggt aagacaccgc aatcggattg
     7441 ccctgcgcgg cgttgcgaag caccaaggcg gtggggctgg cgccgacgct gcgctcgcct
     7501 gagacgagca cggtgacagt gtcgatgatg gagcggccga catgcggctt ttgcgcggcg
     7561 agctgctcga aataatccca gccatataat ttgttcatct gcaccaccca ggtgccgaca
     7621 aaaccgctga atccgggatg gccgaccacc gactggctct tccatttggg atcgatgaac
     7681 tccttccagc ttttcggcgc ctgctccggc ttcactttat tggtgttgta ggcgaggctg
     7741 acggtggtgg cattgacgat ctggtaggcg tcgtcgggat cgacgcccct aaagcgcgcg
     7801 tcgatcgcat cgatcgactg catcttgtag ggcatcaaca ggccgcggtc cttcagcgac
     7861 acgtattgcc ccatgtcggt ggtggcgaac acgtcgcagt tggcggcgcc ggcctggatg
     7921 tcctggctga gccgctgata ggcgacctgc gcggtcgacc gcaccacgtt gaccttcacc
     7981 ccggggaagg tcttggtgaa aatcgccgaa tgcttctcgg aacgatccga cggccagtac
     8041 gcgatgtacc aggtcagttc gccttccagc ctggccttgg cgtagagttc ggcgatgtag
     8101 tcgctctgcg cgcgcgcagg gcgcaggctc atcaacgcgg cggcgctccc ggccacaaag
     8161 cccctccgat ccatcggcat gcgcgctccc tgacaacgct gcggacgctc ttcggcgtct
     8221 cggcgaattt cagttcagca ttgatttagc gcgcgcgcaa atggtgttgc atgcctaacg
     8281 cggaaacccg gccatgcaga cgcggtgatc tcaggcaggg ccgctttact gcgcgaggtg
     8341 cgccgtcaac ggatgattgg ccgcctgact gaagaacgcc gcctcttcgg cggtggcctc
     8401 cagcagctgt tcgtcctggt cgtagatgtc gttgcggaac tgaaccgtct ggtctttggt
     8461 catccacccc gtcagataga tccaggctac cgggactttt ttgggcagga cgatatcctc
     8521 gcgctggccg gtggcgatgg ccgcgtcgat ggcggcgcgg ttccatttcg gcatctcctc
     8581 ggacagcagc caggcggcga ggtcgcggac gttgtcgacg cgggagcagc cgtgcgagtc
     8641 gaagcgatag tcgtcgctga acagattccg ctgattggtg tcgtgcatgt agaccgagta
     8701 cgggttaggc atgtcgatct tcaccgcacc cagcgcattc cagccgccgt tttgctggcg
     8761 caccgtgaaa ttcggggtgt gcgcaccgga ccaatccacc gatcgcggat cgatcggagc
     8821 ctcgtgcgca tcgagcacct ccatatgcat gcgggcgaga tagcccggat ctctccgcat
     8881 atgcgccgag atttcggttt tcgcgatcga tgacgggacg gtccaggtgg ggttgagatt
     8941 gacgctggtg atttcagcgg tcagcgtcgg cgacggcttt tcggtcttgc cgacgatcac
     9001 gcgataacgt cgcaccacat gatcgttctc gaccgcctcg gcaaatgccg cgggcaggtt
     9061 gaccacgacg tagcgctggc cgaatgcgaa attcatattc tcgaggcgct ccagcgaagc
     9121 ctcgagttgc ttgatccgtt tctgcaccgg gacattgagg gcggcgaggg tgcgtggcgt
     9181 caccgttccc gtcggcgcga gaccgtgccg cgcctgaaaa cgcttcacgc cttcggcgag
     9241 gacgtcgtcg taggcaccgg acgccttgtc cgggtcgaga tcgccgctga tcaacaggcg
     9301 ctggcgcagc agatcgtcat gcggaccctg cgtgccggat gcgaatttgg cgtcggccgg
     9361 tatcgccggc cacccgccgc gcaccgcaag gtccgaatag ctcagcgccg cctccctgat
     9421 ccgctgcgcg gtcccctcgt cgaaggtcgg ttcgtgcgac agcgccaagg ccgcggcaga
     9481 gcgcgactcc ggggtggatg cggctgccgg cttggctggc ttgaccggtt tggccgggtg
     9541 cggtgcggcg ccggagtcgg ccatatccgt gcccgcgttg cctgcgccct gggcaaacgc
     9601 cgacgcggtc gcaagtatcg tggcaatggc aatgagtgtc attcttcgca tgttggatgc
     9661 ccttgttggg tgcccctggg acgcgccctg acggcgctga cgttgaaatc aacaagcccg
     9721 gccgtggagc cgaacgaacg ggatcggacc tgacctaaaa acgggttttc gtaaatggtg
     9781 ccttgcgagc gttgcgttcc gatcaggttg gtattgcggc tattgcattg ccgcaagtgc
     9841 cgtattggat tttcgcggct ttcggtatgt cgccgcggcg gctaatgtgg ttcgtgggcg
     9901 cacccgccgg ctggaaaata agcgtgcgtt ttgggaggac ctatgtcgtt gacgcgccga
     9961 gcggcgctcg ctggcggcgg gctggccatg ctgacgggac tgccaaccgt ttctcaacca
    10021 ggcgttgctc aaccaggcat tgctcaacca gtcctggccc aaccaactcc ggcccgggca
    10081 gcctatccca gccgaaccat caagatgatc gtgccctatc cggcgggcgg caccaccgat
    10141 tttctcggcc gcctggtcgc cgatcagctc aagaccggtc ttggcgccac cgtcatcgtc
    10201 gagaacaagc ccggggccgg caccaccctc ggcgccgatc aggtggcaag ggcggagccg
    10261 gatggctacg cgctgctgat ggcgacctcg accacgctgg ccatcaacag gacgctctac
    10321 aagaaactgc cctacgatcc ggtgaaggat tttacgccga tcgcgctggt ggccggcgtg
    10381 ccgttcgcgc tgattgtcaa tccgcaggtt ccggccaaga cgctggcgga attcattgcc
    10441 tacgccaaat cgacgcccgg actggcctat ggctcggccg gcaatggcag tccgcagcat
    10501 ctcggcgccg agatgctgaa ggaggccgcg gccatcgata tccgtcatgt gccgtaccgc
    10561 ggcagtgtgc cggcgatgct cgatgtgatc gcggggcata ttccattcat ggtggtggac
    10621 ctgcagccgg cgttgccgca gatcagggaa ggcaaggtga gggtgcttgg cgtgaccacg
    10681 ccgaggcggg ttgcgatcgc atcggatatc ccgacgctgg ccgaggcggg gctgcccggc
    10741 ttcgaactgg tggcgtggca gggcgtcgtc gcgcccgccg gcgtgccacg ggcgatcgtc
    10801 gacccattgg ccgcgcagat tgcaaagctg atggccgatc cggcaacgcg cgaaaaactc
    10861 accacgattg cgctggagcc gctggcgggg tcgaccccgg acggttttgc ggccttcatc
    10921 aagaccgagg tcgatcggtg gggcgatatc gtcaaaaatt cgggcgccga gccggaatga
    10981 cgagcaccat gtcctcaggt caagtcttcc gcaccgagcg cgatcgatgc ccgaaatgac
    11041 cgagccggat gtcctgatcg ttggcgccgg accggtcggg ctcacgcttg cgatcgatct
    11101 ggcgtggcga gggatcgagg ttacggtggt ggagacgcgt ggccgcgccg cgcctccgga
    11161 gccgaaatgc aaccatgtcg ccgcccgcac catggaaatt tttcggcggc ttggcctttc
    11221 cgaaaaggtg cgcaacgccg ggttgcctgc ggattatccg cacgacgtcg cttatcgcac
    11281 cacgtttacg ggtccggagc tggcgcgaat tccgatcccc tgccgtcgcg accgcttcac
    11341 caggacggat ggcccggact gcaactggcc gacccccgag ccgccgcatc gcatcaacca
    11401 gatatttctc gagccgatcc tgttcgagca tgccgccgcc cagccacgga tccgcatcat
    11461 caaccggaca tcggtcgaag acgtggtggt ggaggatacg tccgcacatg tgaccttgcg
    11521 cgatctcgac accggcgctg tcaggcgttt gagctgtcgg tatctcgtcg gctgcgacgg
    11581 cgcgcgctcc gtggtccgca aggcgattgg cgcggagctg gccggcgacg ccatcgtgca
    11641 gcgcgtgcag tcgaccttta tccgcgctcc cggcctgatc gatcgccagc agcacgcgcg
    11701 cgcctggggc accggcgtca tcaatcccag gcgctccggc atggtgtatg cgatcgacgg
    11761 ccgcgaacga tggctggtgc ataattatct gaaacccggc gagatcgatt tcgaacaggt
    11821 cgatcgcgac ggctgcatcc ggaccatcct cggcgtggga gcggacttcg actacgacat
    11881 catttccagg gaggattggt accggcgccg gctgatcgcg agccggtttc gccacgcttg
    11941 cgcgttcatc gccggcgacg cggcgcatat ctgggtgccc tatgccggct acggcatgaa
    12001 tgccggaatt gccgacgcca tgaacctgtc ctggctgctt gccgcgcatc tcaatggctg
    12061 ggcaccggcc tcgattctcg acgcctacga ggcggagcgt tggccgatca ccagccaggt
    12121 gtcgcgattc gcgatgtcgc acgccgaggc tgaaattcgc cgccgtggcg cggtgcccga
    12181 tgaaatcgag gaggcgggac cgagaggcga acatgcgcgg cgggaagtcg gccgtctcgc
    12241 ctacgagatc aacgtccagc aatatgcctg cgccggtctc aacttcggca cttactacga
    12301 tcgctcgccg atcatcgcct atgacggcgc cgaacatccg gcctatacga tgaacagcta
    12361 taccccgtcg agcgtgccgg gttgccgcac cccgcatctg tggcgcgggg atggtggctc
    12421 gctttacgat gcaatggggc cggagttcac gctgctgagg ttcgatccgg cgctggatgt
    12481 tgtcgcgctg gaggccgcgg ctcgcaaccg gggcgtgccg ctaaaaatcc tcgacatcga
    12541 gccgccatgt accgccattt tccatggcgg cgggctggtg ttgtcgcggc cggaccagca
    12601 tgtggcctgg cgcggcgaca aatcgccggc cgatccgcca gggttgatcg atcgcattcg
    12661 cggagctgca aaccaagaat ttgcaagggc gaaggatcgg ccaacccgtc gtacctagtc
    12721 ttcgttcgcg gcaatcgatt ccatcaggga gaccagctgg cgctgcacgg tctgatcctt
    12781 gatcttgctg taagcccgca atagcctcaa actgaatgcg ctgtcgagga acagcaggct
    12841 ctcgacttcc cgtgccttgc cgtcgccgtc gtagaagaat gtgacgggta catcgagcgc
    12901 ggtcgcgatt tgctgaaggc gggccgcgcc gacgcggttg acgcctttct cgtatttttg
    12961 aacctgctgg aagctgacgc caagtttgtc gccaagctca gcctgcgaga ttttttgctc
    13021 aacgcgccgc agccgaatcc gttttcccaa ctcaatgtcg ggttttcctg cactccgctg
    13081 cttcatcttt tttgctgctg cctgtttcat tctgctttta ccgtccttcg gtcgagaagc
    13141 ccccgaatag atgggtcagg gattcaccct tatataccgc tttaaattcg cttggatgga
    13201 cgaattcgtc cttgaaccac gggaattttg cgggattgaa agcctccacc agccagtcga
    13261 tcatgcgccg taccctgggg atacggccgc tacccggatg ataggagagc caaatatcga
    13321 acggccggcg taactcgatc tcgagcggaa tgatttttcc cccgagctcg caggcgtagg
    13381 aaggaaacac gccgatgccg gccccatagg cgaccgccca gtaattggcg ctgctgacgt
    13441 ttgttttcat cacaagcagc tcctgttggg aataacccgg aaagacgctc tcaaaggtct
    13501 cttttgcggc ggtctggtcg gcgacctgca ttaccagccg gtgcttggcg aagtcgtcca
    13561 ccgttcgcgg gacgccgtaa gtatcgatgt atttctggct ggcaaagaac atcagatgca
    13621 ttcggccgag ccggaccagc ttgacgtcgg gcgcggccgg tcgcgacaaa tggatcgcca
    13681 cgtcggcctc atgccgcgac acgtcggcag agcgcatcgc gcaatgcaga tcgaccagga
    13741 tgttgggaaa cgattgctgg aattcgacca ggcgaggggc tagccagaat gtacccaggc
    13801 cctcggtgac ggcgacccgg acttcgcccg aaagcgtatg ggtgacggat ttgctggcgc
    13861 gcagcaggtc gaacgaagcg gcctccatgc gttcgaccgc cgaaacaacc agcgagcctt
    13921 cgtcggtcag gcgagtgcca tggacgtcgc gcgtgaacag cgtggcgccg atctggcgct
    13981 caaattcgtc aattcgcctg cgaacgacgt tgatggaaag atcaagccgc tcggccgcgg
    14041 acctgaaact tccacaacgg accacctcca gaaaaactct ggccgcatcc caatccgtga
    14101 gacttcctat cgcgagcttg tggcgttcct cctgaggaac gcccctttcc agcaccagac
    14161 gcatacacaa ccctctcagc ccgctaaact tggcagaatt tcgcccgaac cacaactatc
    14221 gcgggtctag ggatttccat tttgacgctt atacttaccg accggggcgg catccccggg
    14281 attttaccga cttcggcctc ccgccgtctt aaccattgaa agaccatcga aagggcagcc
    14341 gtgagttcaa ttgttagcct tcggaccttc gcaaactcat cgaacgttcc gattgtcgtc
    14401 ttggcggaca tgcagcagga atatccggcg aaaccgcggc cgctctcgat ttcaaagatc
    14461 aatcgcgcgc tggacaattg ccggcgggtg ctggatcatg cgcgcgatat cgggctgccg
    14521 gttgcattca tgcgccagct caatgagtcg gcgtttttca accgcgcaac acccttcgtc
    14581 cgctggatcg agggctttga accctatcgc aacgagatgg tattcgaacg cagcaatccg
    14641 tcgtgctact cttgcgagcc cttcgccgcc ttgatgaacc agagccgggg cggcatcgtg
    14701 ctcgcgggat ttgccggcga gtcggcctgc ctgtcgacgc tgatcgatgc gttccaccgc
    14761 aatcacaggg tcacttattc atgcgatgcg tcggccagtc atgcgctcga ggacgtgccg
    14821 gcgaacgaaa ttcaccgtgc cgtttcgaag atatccggac tttatggaga ggtgtatgaa
    14881 acgaccgatt ggattgcctc gactgtacct cgcaagctcg gtgtcggata agacgctggt
    14941 ggctagttcg gaagaaagat tgcattcggt ccttgcggcc ctcgagcagt gccgggcggt
    15001 tctcaccgac agcgctgacc gggagacggc gcaattgctg tcggttgcaa ttcttcagct
    15061 tcggatgaaa ctcaatcagg tctcggacca ggatttgaag gcgttgtgcg atgcgatggt
    15121 gccggatgaa gatcccgcgg aagcaacgca agatccgaaa tcctcgcaaa acgagcggcg
    15181 gcgtgcctcg ctgaagctgg tcaaatagca acatgcacgg gtttccggcc ggcgccgctc
    15241 atcgctggtt gccgagccgc ggcgttagag catgatccgg aaagtggaaa ccggttttcc
    15301 gaaaagatca tgctcaaaca aagagatgag atcctgatcc gattcaatct attcggatca
    15361 tgatccagtg ctagcgcgga atagtgtacc cgcacctcct ggtgcactga ggtgatcgac
    15421 gcggatgcaa ggttgcgcac ctgccgccgc gcctgatcaa aatctcttgt ccgcgggagc
    15481 ctgcggatcg tccctgatct cagcccgccg tcacgtccac cagttcgtcg ccgtggagca
    15541 cgcggtgcgc cttggcgcgg tcgagatcgc cttcccaacc cgcaacaacg acggtagcca
    15601 cgcagttacc gatcagattg ccgagcgcgc gggccatgcc gatgaaccag tccactgaaa
    15661 gcaccagcac gagaccgatc gcgggaatac tcggaacggc attcagtgtg gcggcgagga
    15721 tgacaatcgc cgaaccggga acgccatgcg cgcccttcga cgtgaccagc gagacgccca
    15781 gcaccagcat caggtccccg aaggaaagcg gcgtattggt ggcctgggcg atgaaaacga
    15841 ccgcaagcgt caggtagatc gagaacgcgt cgagattgaa cgaatagccg gtcgggatca
    15901 ccagcccgac gacggaatcc ttcacgccca gccgctcaag tttgcgcatg atctgcggca
    15961 gcaccgcgtc cgacgacgcg gtcgccagaa caatcgtcag ttcctcgcgc agatagccca
    16021 ggaacttgaa gatgttcagt ccgaccagcg ccatgacgct gccgaggacg ccgagcacga
    16081 agaccgccag cgacacataa aacagggcaa ccagcgagat cagctgcttg agcgagccga
    16141 caccgtattt gccgaccgta tagccaaccg ctcccagccc gcccagcggg gccaaacgta
    16201 cgatcaaccc catcgcccgg aacagaacgg tcgataccgc gtcgatcagc gagccgatgc
    16261 gctcggcctt ttctcctccg accagcgcga ggctgacgcc gaaaatgatc gcgaaaaaca
    16321 gcacctggag cacgtcgttg cgcgacagcg cgtcgaaaga ggtggtcgga atgatattca
    16381 tgaggaagct gccgatgccg ccgccctgca gcttgtgggc gttgtcggcg taactgctga
    16441 gcgccttggg gtccagcgtc gaggtatcga tgttcatgcc gtggcccggc ccgaacaggt
    16501 aggcgagcaa caggccgacg accagcgcga ccgtggtcat cacctcgaaa tagatcagcg
    16561 ccttgacgcc gacccggccg actttcttga ggtcgccggc gcccgcgatg ccgtgcacca
    16621 ccacgcagaa cacgatcggc gcgacgatca tcgaaatcag cttcaagaag gcgtcgctca
    16681 agatcttgag ctcgacggca aaatccggca ccgccatgcc aagcacgatg cccagcagca
    16741 aagcgaccag gacctgaacg aaaagtgatt ggtagagagg ttttcggacg cttaccgcag
    16801 tcgcagagat ggttgacatg acttgctccc cctatcgatt ggccttactt cgagcaacaa
    16861 atgtgccaaa cgggcgccca aatcgtgtag cggaaatatt ccgcgacacc attgcgattc
    16921 cagcgggttt tctcgcagat tgcaaaatcg ccgggaccgg gaagccgttg gacggtgcca
    16981 ccggccaagg cccggatctt cacgccggtt ctgctcaaca gggtgcgacg actgcatgat
    17041 tcacgatgtc tcgattcccg ccgcgctgat tgccggtctt gtcagcttcc tgtcgccgtg
    17101 cgtgctgcct ttggtgccgc cctatctggt gtacctgacc ggcgcgacca tcgagcatgt
    17161 cgtcaatgat gaaacggtat cgtcctcgaa gcgcgcggtg atgggtgcgg ccgcgatgtt
    17221 cgtgctcggc ttttccaccg tgttcgtggc gctcggcgcc agcgcctccc tgatcggagg
    17281 gttgatccgc gcctggtcgg gagaactctc gatcgtcgcc ggcatcgtca tcatcatcat
    17341 gggcctgcac ttcctcgggc tgacgcggat cggcttcttg atgcgggaag ggcggttgcc
    17401 gattccaaag ccggtgggct tgtggggggc ctatgtgatg gggctggcgt ttgcgttcgg
    17461 ctggacgccc tgcatcggcc cgatcctggc ggcgatcctg tcggtggcgg cggccgaggc
    17521 gaccgtgacg aaaggcgcgg gcctgctcgc ggtctattcc gcagggctcg gaattccatt
    17581 tctgttggcg gctttcatgg ttgaacagtt ctcgtcgctg ttctcgcgga tgaagcggca
    17641 tctcgccgcc gtcgagcacg ccatgggggt gctgatggtc atcaccggga tcggctttct
    17701 caccggcgcc gcatccaacg tcagcatctg gctgttggag acgtttcctg gcctgcagaa
    17761 tttcggctag ggcgcggact cacaagcact tggcgcctgc gatgcaggct ccatgctgct
    17821 acatgcgcac gacggatgta tcaatcatcc gcgccgcgcc gtcgtgagcg gcggagcatc
    17881 ggttcgatgc cgcttcattc atacgcgctg agttcgtagc gcatgattcg aggctccagt
    17941 ttgagcgctt gaatcaggtc acggcgtacc atcaacctgc aaacgcccgt cgcggacgct
    18001 gctaatgctg tgagtttact tcggctttcg ggcatagtgg acatggccgg accttccgct
    18061 ggctagccca acttgcgcag aatcgcaccc tgactataag caaatcaatg ccttagccga
    18121 aaagttggca ctacattttc ttgggaattg tgatcaggcg ctgtgttgga gagcaggcaa
    18181 atcttgctcg gtgaggcgca ttcgctt
//
LOCUS       DATNOI010000528         9009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2543912,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000528 DATNOI010000000
VERSION     DATNOI010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543912"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..108)
                     /locus_tag="VN968_21390"
     tRNA            complement(32..108)
                     /locus_tag="VN968_21390"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72..74),aa:Ala,seq:tgc)
     gene            complement(182..877)
                     /locus_tag="VN968_21395"
     CDS             complement(182..877)
                     /locus_tag="VN968_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXL31824.1"
                     /translation="MDDWIDYYDSTHTIYASKRHRDLHFQIIAQDIVGYITSLDAVVL
                     DYACGEALSAARVAEACAKLFLAEPAPGVRGRLIARFAPNTKIRVRSLDDLRNMEEKS
                     IDLAIMNSVAQYMTPHELDAAFAVVRRLLKPGGRLVVGDIMRPEVGMGRDVIALLHFA
                     QTHGFLKDAMVGLISTALSEYRLLRSRVGLQRYSETEMVAKLAAAGFTATRTLANIGH
                     NPWRMTFIARQAV"
     gene            1004..1531
                     /locus_tag="VN968_21400"
     CDS             1004..1531
                     /locus_tag="VN968_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HXL31825.1"
                     /translation="MSKAKSDKVSPDLDTPSDLPQAAVDKISAALNTLLADAFALYLK
                     TKNFHWHVSGRHFRDYHLMLDEQSDAIFATTDQLAERVRKLGGTTMRSIGQIGKLQTI
                     KDNDESFVPPRDMLRELMEDNKHVAAAMRKAHKLCDDHEDSGTASLLEVFIDETERRT
                     WFLFEASRQEGSNEA"
     gene            complement(1616..3070)
                     /locus_tag="VN968_21405"
     CDS             complement(1616..3070)
                     /locus_tag="VN968_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HXL31826.1"
                     /translation="MLRGMLTLTLSLCLGAAAAPAALAQAAAPITEQEAHAIAVDAYV
                     YFYSLMSMDVSRKQFTNGTTDFKGPMNTFVNVPEYPPASFKGVVRSNFDTLYSVSWLD
                     MTKEPVVITAPDTDGRYYLLPMLDMWTDVFASPGWRTTGTKAGTFLVTPAGWRPDLRD
                     RFADEFKLPKDTQRIEAPTPYVWVIGRTKTDGPADYDAVHKIQAGYKVTLLSEFGKTP
                     KPVEFKLDPSVDMKTPPKVQVDTMAAGAYFAYAAELLKLHPPHVTDEPIIAQMKKIGI
                     EAGKSFDIGKLDPAVQRALETAPQDGQKLMAWKVPTLARVANGWSMNTDTMGVYGNYY
                     LKRSIVSQVGLGANLPEDAVYPLNLGDESGKPLDGANKYTITFEKGAAPPVNAFWSIT
                     LYDQEGFQVGNALNRFAVSSWMPFKNNADGSLDIYFQNESPGKDKEANWLPAPKGSFN
                     LTMRLYAPKSEALTGKWNPPPVVKTPETVGLSAQ"
     gene            3370..4479
                     /locus_tag="VN968_21410"
     CDS             3370..4479
                     /locus_tag="VN968_21410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HXL31827.1"
                     /translation="MIIILCLYIVALWLVFSKFKLVRWGWLSGTVSVLVGVFILATFL
                     ALFNYLTPSGRVTVTGRVVEVTPNVTGQIVSIPVKPNVPVKANDVLFQIDPAPFQYKV
                     AQLQASLAAAKQQTEILKSNYEQATANVVGLTAQVAFNAKRNADILKLATEGANTQFQ
                     AQDRQNQYETALAQLNVAKAAQQSAKLALDSEIGGVNTTVAQTQAQLDNASWELSQTA
                     IRAPADGYVTLVTLSVGDRALQARSAMSFIVENEITLVGMFSQNGFQTVKDGAPVDIV
                     FDNAPGRIYHAKITAIPKGVGQGQVAVSGTLARTSALGGTTVFPAEISIPDGMSHESL
                     RLGMSGSATAFADNAGVIGLLASILVWISSYTAYL"
     gene            complement(4498..5031)
                     /locus_tag="VN968_21415"
     CDS             complement(4498..5031)
                     /locus_tag="VN968_21415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31828.1"
                     /translation="MTVVSADIEPLAFTFEDDGLVPNNPMPFLVYKQAISVDNEHPEK
                     TIEGLFGANGWGEMWRNGVYDYLHYHATVHEVLGVARGHARVRFGGDAGKEIEIAAGD
                     VAILPAGTGHQCLSASEDFCVIGAYPPGAKMQITLPTPENYRKALKTIPEVKLPKTDP
                     MRGENGPLARLWGRTNS"
     gene            complement(5164..6354)
                     /gene="carA"
                     /locus_tag="VN968_21420"
     CDS             complement(5164..6354)
                     /gene="carA"
                     /locus_tag="VN968_21420"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HXL31829.1"
                     /translation="MTTPENASAWPDHQPTALLVLADGTVLEGFGLGAEGQAVGEVCF
                     NTAMTGYEEILTDPSYAGQLITFTFPHIGNVGTNDEDIETVNMAATPGARGVILRSAI
                     TDPSNYRATRHLDLWLKARGIIGLSGIDTRALTALIRGKGMPNAVIAHAKDGEFDLHG
                     LKEEAREWPGLEGMDLVPMVTSGQRFDWDETPWAWEKGFGRQTEPEFNVVAIDYGIKR
                     NILRLLAGVGCKITVVPATTSAEDILAMKPDGVFLSNGPGDPAETGKYAVPVIKKVIN
                     SGTPTFGICLGHQMLGLAVGAKTKKMHQGHHGANHPVRDETTGKVEITSMNHGFAVDQ
                     ATLPKGATQTHVSLFDGSNCGISLDGKPVFSVQYHPEASPGPRDSHYLFKRFADLMRA
                     KKSG"
     gene            6580..7035
                     /locus_tag="VN968_21425"
     CDS             6580..7035
                     /locus_tag="VN968_21425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HXL31830.1"
                     /translation="MLRDDINNAVKDAMRAKDERKLSTLRMVNSTIKNADIAARGEGK
                     PPLSDGDLLGVFQKMIKQRQESVELYDKGGRAELADQEREEIAIISAYLPKQMSEADV
                     KAAIASVIAETNAAGMKDMGKVIAALKAKYAGQMDFGKASGMVKAALSS"
     gene            7259..8782
                     /locus_tag="VN968_21430"
     CDS             7259..8782
                     /locus_tag="VN968_21430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL31831.1"
                     /translation="MADQQAPPAGPDLSQGVALSEFSGATLLGHVGDEDVLLVRSGSG
                     VFAIDAHCSHYHGPLAEGLADGESIRCPWHHACFDLRTGEAARAPALNSIAVWKVEHE
                     GDRIFVRQKREQPKPRVKGAVDAPGRIVIVGGGAAGFAAAEMLRRQDYRGSIAMLSNE
                     AAAPVDRPNLSKDYLAGSAPEDWLPLRPDGFYADAAIDMRLSTEVASIDTKARAVVLA
                     GGEAIAYDRLLLATGAEPVRLPIPGADQPHVHVLRSLADCRAIIASARGARRAIVIGA
                     SFIGLETAAALRARDIEVHVVGLEQRPMDRVLGPEMGDFVRALHEQHGVIFHLGDTVT
                     AIDGKRATLRSGGVLEADVVVVGVGVRPRFALAEKAGLAIDRGVTVSAYLETSIPGIY
                     AAGDIARWPDPHSQENIRVEHWVVAERQGQTAARNMLGQREAFDAVPFFWSQHYDVPI
                     NYVGHAEKWDEIVVDGNIAAKDCLLQYRSGGRVLAVASIYRDVASLEAELAMEQATH"
BASE COUNT         1731 a         2679 c         2810 g         1789 t
ORIGIN      
        1 tgccgagccg aagctgcaac gcagcgaagg ctggaggccc ggcctggatt tgaaccagga
       61 tatagagcga tgcactgctc tcgcgtggaa gctttccgcc accgggccgc gccgatcatg
      121 gccgatcacg ccacggtgag ccacgcttcg cgtcgattaa ccctaatcgc cggttggaaa
      181 atcagacagc ctgccgcgcg atgaacgtca tccgccacgg attgtgcccg atattggcaa
      241 gggtgcgggt ggcggtaaag ccggcggcgg cgagcttcgc caccatctcg gtttcgctgt
      301 aacgctgcag cccgacccgc gagcgcagca gccgatattc cgacagcgcg gttgagatca
      361 gcccgaccat ggcgtctttc aggaagccgt gggtttgcgc gaagtgcagc agggcgatca
      421 cgtccctgcc catgccgacc tccggacgca tgatatcgcc caccaccagc cggccgccgg
      481 gtttcagcag ccgccggacc accgcgaatg cggcatccag ttcgtgcggc gtcatatatt
      541 gggcgaccga gttcatgatc gcgagatcga tggatttttc ctccatgttt cgcaggtcat
      601 ccagcgagcg caccctgatt ttcgtgttcg gggcaaaacg cgcgatcagt cggccgcgca
      661 cgcccggcgc cggctcggcc aggaacagtt ttgcgcaggc ctccgctacc ctggccgccg
      721 acaaggcctc gccacaggcg tagtccagca ccacggcgtc cagcgaggtg atgtagccga
      781 cgatatcctg ggcgatgatc tggaaatgca ggtcgcgatg ccgcttgctc gcgtaaatcg
      841 tatgggtgga atcgtaataa tcgatccagt cgtccatcgc aaaggtgtcc ggcaaaaaag
      901 ggaggttccg gcagcggaac cggtggctac atgatgcgtt agatcgtccg gctacccgtg
      961 tccatcaccg tcaaagtccg gtttcctagc aggaaggttc atcgtgagca aagccaagtc
     1021 cgacaaagtc tctcccgacc tcgatacgcc gagcgacctg ccgcaggccg cggtggacaa
     1081 gatttcagcg gcgctcaaca cgctgctggc tgacgccttc gcgctctatc tgaagaccaa
     1141 gaactttcat tggcacgtca gcggccggca tttccgcgat tatcatctga tgctcgacga
     1201 gcagtcggat gcgattttcg ccaccaccga tcagctcgcc gagcgcgttc gcaagctcgg
     1261 tggcacgacg atgcgctcga tcgggcagat cgggaaattg cagaccatca aggacaatga
     1321 tgagagtttc gtgccgccgc gcgacatgct gcgcgagttg atggaagaca acaagcacgt
     1381 ggccgccgcg atgcgcaagg cgcacaagct ttgcgacgat catgaggatt ccggcaccgc
     1441 gagcctgctc gaagtcttca tcgacgagac cgagcgccgc acctggttcc tgttcgaggc
     1501 cagccgccag gaaggcagca acgaggcgta ggcgcagcgc tgctcttcac aggtcgctct
     1561 gcggagagaa ataaatcgga tgccggcgcc gaagcaccgg cgctccagtt aatttttact
     1621 gagccgacag gcccaccgtt tccggcgtct tcacgaccgg cgggggattc cattttccgg
     1681 tcagcgcctc tgacttcgga gcgtagaggc gcatggtcag attgaaggat cccttcggcg
     1741 ccggaagcca gttggcttcc ttgtccttgc ccggactctc gttctggaag tagatatcga
     1801 gcgaaccgtc cgcgttgttc ttgaacggca tccagctgct gacggcgaag cggttcagcg
     1861 cgttgccgac ctggaatcct tcctgatcgt aaagcgtgat cgaccagaaa gcattgaccg
     1921 gcggggcagc gcctttctca aacgtgatgg tgtacttgtt ggcaccatcc agcggtttgc
     1981 cggactcatc gccgagattg agaggataga cggcgtcctc gggcagattc gcgccaaggc
     2041 ctacctgcga gacaatggac cgcttcaggt agtagttgcc gtagacaccc atcgtatcgg
     2101 tgttcatcga ccagccattc gcaacccgcg ccagcgtcgg cactttccag gccatcagct
     2161 tctggccgtc ctgcggcgcg gtttccagcg ctctttgcac ggctggatcg agcttgccga
     2221 tgtcaaagct cttgccagcc tcgatgccga ttttcttcat ctgtgcgatg atcggctcat
     2281 cggtgacatg aggcggatga agcttgagca attctgctgc ataggcgaaa tacgcaccag
     2341 ccgccatcgt atcgacctgg acttttggcg gcgtcttcat gtcgacgctt ggatccagct
     2401 tgaattcgac cggcttgggt gttttgccaa actcggaaag aagggtgacc ttatagccag
     2461 cctgaatctt atggacggcg tcataatccg ccgggccgtc ggtcttggta cgaccgataa
     2521 cccagacata aggcgttggc gcttcgatcc gctgagtgtc ctttggaagc ttgaattcgt
     2581 cggcgaacct gtcgcgcaga tccggcctcc agcccgcggg cgtcaccaga aacgtcccgg
     2641 ccttggtgcc cgtcgttcgc cagcctggcg atgcaaaaac atcggtccac atgtcaagca
     2701 tcggcagcag gtagtagcgt ccatcggtat ccggagccgt gatgacgaca ggctccttgg
     2761 tcatgtccag ccaggaaacg gaatagagcg tatcgaagtt ggaccgcacg acgcctttga
     2821 aactcgccgg tgggtattcc ggcacgttga cgaacgtgtt catcgggcct ttgaaatccg
     2881 tggtgccgtt ggtgaattgt tttcgggaca catccatcga catgagggag tagaagtaga
     2941 cataggcgtc caccgcgata gcgtgcgcct cctgctcggt gattggcgcc gccgcctggg
     3001 ccagcgccgc gggcgcagcc gccgcgccaa ggcagagaga caaggtcagt gttagcattc
     3061 ctcgcaacat ttcgaacact cctcttctgg ttgtgatacg cttgacatcc attccttccg
     3121 caaaaatcgg tcgaacgatt tggaagcatt cccgctctga cgcgagaggt tgcgtattgc
     3181 ttgcaagaga atgtacaccg ccggctgcat aaaacgccgc gcggcgttcg cagaatcgaa
     3241 caaggtcgaa aaaaatagat gctattaaag ctgactgtcg aagggcgcct cttcggttgc
     3301 ctctacctgt aatacgcagc cttctatcag gggtagtacc ttagcacgag tagataatga
     3361 gggccagtca tgatcattat tttatgcctt tacatcgtcg cgctctggct cgtgttttca
     3421 aaattcaaac tggtgcgatg gggctggttg tccgggacgg tctcggtgct tgtcggcgtg
     3481 tttattctcg caacttttct cgcgcttttt aattatctca cgccgtcggg gcgcgtcacg
     3541 gtgaccggtc gggtggtgga agtgacgccg aacgtgacgg gacaaattgt ctcaatcccg
     3601 gtcaagccga acgtgccagt gaaggcaaac gacgtgctgt tccaaatcga cccggcgccg
     3661 tttcaataca aggtcgcgca attgcaagcg tcgctcgccg cggcaaagca acagactgag
     3721 atcttgaaat cgaattacga gcaggccacc gcgaatgtcg tcggtctgac cgcgcaggtc
     3781 gcgttcaacg caaaacgcaa cgcggacatt ctgaagcttg caacggaagg cgccaacacg
     3841 caattccagg cgcaggaccg ccagaatcaa tacgagacgg cgctggcgca gctcaacgtg
     3901 gccaaagcgg cgcagcagag cgccaaactc gcgctggatt ccgagatcgg cggcgtcaat
     3961 acgacagtcg cgcagactca ggcgcagctc gacaacgcga gctgggaatt gtcgcagacc
     4021 gccattcgcg caccggccga tggctatgtg acgctcgtga cattgtcggt cggcgaccgg
     4081 gcgctgcagg cgcgctcagc gatgtccttc attgtcgaga acgagatcac gctcgtcggc
     4141 atgttttcgc agaacggttt tcagaccgtc aaggacggcg cgcccgtcga tatcgttttc
     4201 gataacgccc ccggacgcat ctatcacgca aaaattacgg caattcccaa gggcgtgggt
     4261 caaggacaag tggccgtgtc gggaacgctg gcgcgcacca gcgcgctcgg tggcacgacg
     4321 gtctttccgg cggagatatc gatacctgac gggatgagcc atgaatcact tcgtctggga
     4381 atgtccggca gcgctaccgc atttgccgac aacgcgggcg ttatcggtct tctcgcgtcc
     4441 atcctcgtgt ggatcagttc ctacacggcg tatctctagc cattcgccgc ttgagagcta
     4501 ggaattcgtc cgaccccata atctggccag cggtccattt tcgcctcgca tcgggtcggt
     4561 cttcggtagc ttcacttccg gaatcgtctt cagcgccttc cgataatttt ccggcgtcgg
     4621 caacgtgatc tgcattttcg cgcccggcgg ataagccccg atcacgcaaa aatcctcact
     4681 cgccgacagg cattgatgtc cggttccggc cggcaggatc gcgacatcgc cggccgcgat
     4741 ctcgatttcc ttgccggcgt cgccgccgaa ccggacccgt gcatggccgc gcgcgacgcc
     4801 gagaacttca tggaccgtgg cgtggtagtg cagataatcg tagacgccgt tgcgccacat
     4861 ctcgccccag ccattggcgc cgaacagtcc ctcgatggtt ttctcggggt gttcgttatc
     4921 gactgatatc gcctgcttgt agaccagaaa cggcatcggg ttgttcggga cgaggccgtc
     4981 gtcttcgaac gtgaaggcga gcggttcgat gtcggcgctg accacggtca tggcaattct
     5041 ccgcagttga aataagcgcg gattcaacgg ttgtcagcgg ctcatgttcc cttcgatcac
     5101 gccttgggaa tctcgaaaac tggattgctt cgtcgcttgc gctcctcgca atgacggcgt
     5161 cgcttacccg ctcttcttcg cccgcatcag atccgcgaac cgcttgaaca gataatgcga
     5221 gtcgcgcggg ccgggggacg cttccgggtg atactgcacc gagaataccg gcttgccgtc
     5281 gagcgagatg ccgcaattcg agccgtcgaa cagcgacacg tgggtctgcg tcgcgccctt
     5341 cggcagcgtc gcctgatcca ccgcgaagcc gtggttcatc gaggtgattt ccaccttgcc
     5401 ggtggtctcg tccctgaccg ggtgattggc gccgtgatgg ccctgatgca tcttcttggt
     5461 tttggcgccg accgctaggc ccagcatctg atggccgagg caaatgccga aggtcggcgt
     5521 gcccgaattg atcaccttct tgatcaccgg caccgcgtat ttgccggtct cggcgggatc
     5581 gccggggccg ttggagagga acacgccgtc gggcttcatc gccaggatgt cttcggccga
     5641 tgtggtcgcc ggcaccacgg tgatcttgca gccaacgccg gcgagcagcc gcaggatgtt
     5701 gcgcttgatg ccgtagtcga tggcgacgac attgaactcg ggctcggtct gccgcccaaa
     5761 ccctttttcc cacgcccacg gcgtttcgtc ccaatcaaag cgctggccgg aggtgaccat
     5821 cggcaccagg tccatgccct cgaggccggg ccactcgcgg gcttcttcct tcagcccgtg
     5881 caggtcgaac tcgccgtcct tggcatgggc gatcacggcg ttcggcatgc ccttgccgcg
     5941 gatcagcgcg gtcagcgcac gggtatcgat accggaaagg ccgatgatgc cgcgggcctt
     6001 cagccacaga tcgaggtgtc gcgtggcgcg gtagttcgac gggtcggtga tggcgctgcg
     6061 caggatgacg ccgcgcgcgc ccggcgttgc cgccatattc accgtttcga tgtcctcgtc
     6121 gttggtgccg acattgccga tgtggggaaa ggtgaaggtg atgagctgtc cggcatagga
     6181 gggatcggtg agaatctcct cataaccggt catggcggtg ttgaagcaga cttcgccgac
     6241 ggcctggcct tccgcgccga ggccaaagcc ttccagcacg gtgccatcgg caagcacgag
     6301 gagcgcggtc ggttggtgat ccggccaagc tgaggcattt tcaggtgttg tcatgaacgc
     6361 attacatagt cgccgcattc ggcggcgtca aagccggaaa gcatggattc acatgccaaa
     6421 tacccccatt ttgagccggc ctcatgccga ggagcgcgga acggcggccg atgccctgga
     6481 tcggatgtta cggatccgag ttgtctgatt gccgcgaacc gcctagatca ggggcttcca
     6541 ggcaagcggc cttaaagggc ttcgcgagag gacatgacga tgctgcgcga cgacatcaac
     6601 aacgcggtca aggacgcgat gcgggccaag gacgagcgga agctttcgac gctgcgcatg
     6661 gtcaattcga ccatcaagaa cgccgacatc gccgcacgcg gggaaggcaa gccacccctg
     6721 agcgatgggg acctgctggg cgtgttccaa aagatgatca aacagcgcca ggaatccgtc
     6781 gaactgtacg acaagggcgg ccgcgccgaa ctggccgatc aggagcgcga ggaaatcgcg
     6841 atcatctccg cctatctgcc gaagcagatg tcggaggccg acgtcaaggc ggcgatcgct
     6901 tccgtcatcg ccgagaccaa tgccgccgga atgaaagaca tgggcaaggt gatcgcggcg
     6961 ctgaaggcga aatacgccgg acagatggac tttggcaagg ccagcggcat ggtgaaggcg
     7021 gcgctttcct cttaacctcg ccccgcttgc ggtgagaggt tgaagccgga gcgaagcgac
     7081 ggcttcgggt gagggggact ctccgcgatc acggtgtttg tggtaataat gggcctgtcc
     7141 taattcccga atacgaacgt tacccagtgc aggctgggtg ccgccggatg gtagacaggg
     7201 ggcaagatgg tcgcggcgct gaggacgaaa atgcgctgga actcaaggga ggtaagacat
     7261 ggccgatcaa caagccccac ccgccggtcc cgatctgtct cagggtgtgg cgctatccga
     7321 attttccggc gcgaccctgc tcggccatgt cggcgatgaa gacgtgttgc tggtgcgttc
     7381 gggatcgggg gtcttcgcca tcgatgccca ctgcagccac tatcacggcc cactcgccga
     7441 aggcctcgcg gacggcgaga gcatccgttg tccctggcac cacgcctgtt tcgatttgcg
     7501 caccggggaa gccgcccgcg ctccggcctt gaactcgatc gcggtctgga aggtcgagca
     7561 cgaaggtgac cgcattttcg tccggcaaaa gcgcgagcag cccaagccgc gcgtgaaagg
     7621 cgctgtcgat gcgcccggcc ggatcgtgat tgtgggcggc ggcgcggcgg ggttcgccgc
     7681 cgccgagatg ctgcgacggc aggattatcg cggcagcatc gcgatgctga gcaacgaggc
     7741 ggcggcgccg gtggatcggc cgaacctgtc gaaggattat ctcgccggca gcgcgccgga
     7801 ggactggctg ccgctgcggc cagacggttt ttacgctgat gccgccatcg acatgcggct
     7861 cagtaccgag gttgcatcga tcgacaccaa ggcgagagcc gtggttctcg ccggcggcga
     7921 agccattgcc tacgaccgtt tgctgctggc gaccggtgcg gagccagtgc gtctgccgat
     7981 ccccggcgcg gatcagcccc atgtccatgt gttgcgctcg ctggccgatt gccgcgcgat
     8041 cattgcatcg gctcgcggcg cgcgccgcgc gattgtgatc ggtgcaagtt ttatcgggct
     8101 tgagactgcg gcggcgttgc gtgccaggga tatcgaggtc cacgtcgtcg ggctggagca
     8161 gcggccgatg gaccgcgtgc tcggaccgga gatgggcgat ttcgtccgtg cgctgcatga
     8221 acagcacggc gtcatctttc acctcggtga tacggtaact gcgatcgacg gcaagcgtgc
     8281 gacgctgagg agcggcggcg tgctcgaggc cgatgtcgtg gtggtcggcg tcggcgtgcg
     8341 gccgcgcttc gccttggccg aaaaggccgg gcttgcgatt gatcgcggcg tcacggtgag
     8401 tgcgtatctc gaaaccagca tccccggcat ctacgcggcg ggcgatattg cgcgctggcc
     8461 cgatccgcat tcccaagaga acattcgcgt cgaacactgg gtggtggccg agcgccaggg
     8521 ccagaccgcc gcgcgcaaca tgctgggaca acgcgaagcc ttcgatgcgg tgccgttctt
     8581 ctggagccag cattacgatg tgccgatcaa ctatgtcggt cacgccgaga agtgggacga
     8641 gatcgttgtg gacggcaata tcgccgcgaa ggattgcctg ctgcagtaca ggagcggtgg
     8701 gcgcgtgctc gccgttgcct cgatctatcg cgatgtcgca agcctcgagg cagaacttgc
     8761 gatggagcag gcgacgcact gacctccttt cccggacggg aggggtttgt gttgcaaggg
     8821 cgatggtcta acatgttgtt ttcattcaac ttaactgtct ctgtcggtcc aatcgacttt
     8881 ggctcgacgg acaatccgaa gcgttagccc aacttgcgca gaatcgcacc ctgactataa
     8941 gcaaatcaat gccttagccg aaaagttggc actacatttt cttgggaatt gtgatcaggc
     9001 gctgtgttg
//
LOCUS       DATNOI010000529         4859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_110754,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000529 DATNOI010000000
VERSION     DATNOI010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110754"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..418
                     /gene="rplK"
                     /locus_tag="VN968_21435"
     CDS             2..418
                     /gene="rplK"
                     /locus_tag="VN968_21435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HXL31832.1"
                     /translation="MTGYLKLQVPAGAANPSPPIGPALGQRGLNIMEFCKAFNAQTQK
                     EEKNTPIPVVITIYADRSFTFELRTPPMSFFIKKAAKLTSGSKMPGRDKAGAVTKAQV
                     REIAEAKMKDLNCDTIEAAMKMVEGSARSMGVEVAG"
     gene            424..1116
                     /gene="rplA"
                     /locus_tag="VN968_21440"
     CDS             424..1116
                     /gene="rplA"
                     /locus_tag="VN968_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HXL31833.1"
                     /translation="MTIGKRLKKAREGVDREKLYPLADAIKMVKERAKSKFDETVEIA
                     INLGVDPRHADQMVRGVVTLPNGTGRTLRVGVFARGAKAEEAKAAGADVVGAEDLVEK
                     VQNGQIDFDRCIATPDMMPLVGRLGKVLGPRGMMPNPKIGTVTMDVATAVKGAKGGSV
                     EFRVEKAGIVQAGIGKASFSEEKLVQNVKALADAVAKAKPAGAKGTYIQRVAVSSTMG
                     PGVKVEPGTVLG"
     gene            1204..2151
                     /locus_tag="VN968_21445"
     CDS             1204..2151
                     /locus_tag="VN968_21445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HXL31834.1"
                     /translation="MPDKVLIYSRFPKAMMVRIGQRFELMDAAGKPPGEVFTAEQLSG
                     VSAMITAGGTPLGAAMMDTMPKLAAIVCYGTGYDGVDLAAAAQRGIAVGHSPAANAAA
                     VADLAVTSMLAVTRRLLPADGYVRSGNWSAAKPSPLMSPQPGNPGRRVGVFGMGEIGR
                     KIASRVAAFETEVGYFSRSRRDVPYRYLPSLAALAEWCSVLMIAVRAGVDTHHAVNAS
                     ILQKLGKDGYVVNISRGSVIDQQALIAALTDKTIAGAGLDVFEKEPHVPDALTGFPNV
                     VLTPHIGGHTIESHMAMQDCVIANLEAYFAGKPLAHPVG"
     gene            complement(2176..3063)
                     /gene="sigJ"
                     /locus_tag="VN968_21450"
     CDS             complement(2176..3063)
                     /gene="sigJ"
                     /locus_tag="VN968_21450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HXL31835.1"
                     /translation="MSSDRPRESDPLAPHRGRLLGLAYRMLGSRSDADDVVQDAYIRF
                     AGAQDVHNPEAFLVTVVTRLCLDRLKSAKAQREIYVGPWLPEPVFDAEGLAADAATEL
                     ADDLSFALLLAVDRLSPLERAAFLLHDVFDVPFSEIARMLDRTEAACRQLATRARRAV
                     RDTRPAPAATPDSHARLLSAFSEAVASGDVSRLAGLLRADAVAVTDGGGRKTAALKEI
                     MGADKVARFFIALAAKNAGRDVRIQPTMINGTVGALLYMDGEVDHTPSMAIDGDRIAA
                     IYIVRNPDKLRRAPASGHR"
     gene            complement(3041..3307)
                     /locus_tag="VN968_21455"
     CDS             complement(3041..3307)
                     /locus_tag="VN968_21455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31836.1"
                     /translation="MRSWIAHRVLRATSKRYGYDTSYLDMMLKEAPSAFFKFASLMKA
                     AIDLTLALQLGRMFPMVKAGLGYAKECRRVSVDGHNFDVIRQAA"
     gene            complement(3300..3677)
                     /locus_tag="VN968_21460"
     CDS             complement(3300..3677)
                     /locus_tag="VN968_21460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31837.1"
                     /translation="MRRSIAGVLAALTTLNGSMMLFAGLTWYASVPGVSETGPYNPHF
                     VQDIGAAFLVAGLALAARAWRPAYWPAAVAGSAFLAAHALIHLAAIVTGQDHHTAIDL
                     IAVVLPSALALFSAFPDPGERHA"
     gene            4299..4817
                     /gene="rplJ"
                     /locus_tag="VN968_21465"
     CDS             4299..4817
                     /gene="rplJ"
                     /locus_tag="VN968_21465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HXL31838.1"
                     /translation="MERAAKKDAVEALNGLFKTTSVAVVAHYSGLTVAQMQKLRMQMK
                     QAGASVKVSKNRLAKIALEGTDVVAIGALLKGPTVIATSNDPVAAPKVAIEFAKTNEN
                     FVILGGSMGKTVLNVDGVKALAALPSLDELRAKILGLLVAPATKIAQLTTAPAAKLAR
                     VVQAYASKSEAA"
BASE COUNT          957 a         1482 c         1581 g          839 t
ORIGIN      
        1 agtgaccgga tacctgaaat tgcaggtgcc ggccggtgcg gcgaaccctt cgcccccgat
       61 cggacccgcg cttggtcagc gcggcctcaa catcatggaa ttctgcaagg cgttcaacgc
      121 gcagacgcag aaggaagaga aaaatacccc gataccggtg gtgatcacga tctacgccga
      181 tcgttcgttc accttcgaac tgcgcacccc gccgatgtcc ttcttcatca agaaggcggc
      241 caagctgacg tccggctcga agatgccggg ccgtgacaag gccggcgcgg tgaccaaggc
      301 gcaggtgcgc gagatcgccg aagcaaagat gaaagatctg aattgcgaca ccatcgaagc
      361 ggccatgaag atggtcgagg gctcagcccg ttcgatgggc gtcgaggtgg cggggtaacg
      421 gtcatgacaa ttggaaaacg cttgaagaag gcccgcgagg gcgtcgatcg cgaaaaactt
      481 tatccgctcg ccgacgccat caagatggtc aaggaacgcg ccaagtcgaa attcgacgag
      541 acggtcgaga ttgcgatcaa tctcggcgtc gatccgcgtc atgccgatca gatggtgcgc
      601 ggcgtggtga ccttgccgaa cggcaccggc cgcaccctgc gggtcggcgt gttcgcgcgc
      661 ggcgccaagg ccgaagaagc caaggcggcg ggtgcggacg tggtcggcgc ggaggacctg
      721 gtcgagaaag tgcagaatgg ccagatcgat ttcgaccgct gcatcgccac ccccgacatg
      781 atgccgctgg tcgggcggct gggtaaggtg ctaggccccc gcggcatgat gcctaatccg
      841 aagatcggca ccgtcaccat ggacgttgcc accgccgtga aaggcgccaa gggcggttcg
      901 gtcgaattcc gcgtcgaaaa agcgggaatc gtccaggccg ggatcggcaa ggcgtcgttc
      961 tcggaagaga agctggtgca gaacgtcaag gcgctcgcgg acgcggtcgc caaggcgaag
     1021 cctgctggcg ccaagggcac ctacatccag cgcgtcgcgg tttcctccac catgggaccg
     1081 ggcgtgaagg ttgagccggg gactgtgctc ggctaaaact ttcgaaaacc acgcaagagg
     1141 aaccagggac ggaattgggc aaccgattcc gtcccttagc tttgatatca gggaaatcag
     1201 tcgatgccgg acaaggtctt gatctattca cgcttcccca aggcgatgat ggtgcgcatc
     1261 ggccagcggt ttgaactgat ggacgccgcg ggcaagccgc cgggtgaggt cttcaccgcc
     1321 gaacagctct ccggcgtcag cgccatgatc accgccggcg gaacgccgct gggggctgcg
     1381 atgatggata cgatgccgaa actcgcggcg atcgtctgct acggcaccgg ttacgacggc
     1441 gtcgatcttg ccgccgcggc tcagcgcgga atcgccgtcg gccacagtcc ggcggcgaac
     1501 gccgccgccg ttgccgatct ggcggtgacc tcgatgctgg ccgtgacgcg gcgcttgctg
     1561 ccggcggatg gatatgtccg aagcggaaac tggtcggcgg caaagccgtc gccactgatg
     1621 agcccgcagc ccggcaatcc cggccggcgc gtcggcgtct tcggcatggg cgagatcggc
     1681 cgcaagattg cctcgcgggt cgccgccttc gagaccgagg tcggctattt cagccgcagc
     1741 aggcgcgacg tgccctaccg gtatctgccg agcctcgcgg cgctggcgga atggtgcagc
     1801 gtcctgatga ttgcggtgcg tgccggcgtg gacacccatc acgccgtcaa tgccagcatc
     1861 ctgcagaagc ttggcaagga cggctatgtc gtcaacatct cgcgcggctc ggtgatcgac
     1921 cagcaggccc tgatcgcggc gctcaccgac aagaccatcg ccggcgccgg tctcgacgtg
     1981 ttcgagaaag aaccgcatgt gccggacgcg ctgaccggct tcccgaacgt cgtgctgacc
     2041 ccgcatatcg gcggccatac catcgagtcg cacatggcga tgcaggattg cgtgatcgcc
     2101 aatctcgagg cgtattttgc tggcaagccg ctcgcgcatc ccgtgggctg acgcgcccga
     2161 tcaggatctg aacgctcagc ggtgtccgga ggctggcgcg cgacgcaatt tgtcgggatt
     2221 gcgcacgata tagatcgccg cgatccggtc gccatcaatg gccatgctcg gcgtgtgatc
     2281 gacttcgcca tccatataga gaagggcacc cacggttccg ttgatcatcg tcggctggat
     2341 gcgaacatcg cggccggcgt tcttggccgc gagggcgatg aagaaacgcg cgaccttgtc
     2401 ggcgcccatg atctccttca gcgccgcggt tttgcggccg ccgccatcgg tcactgcgac
     2461 agcatctgcg cgcagcaggc cggccagacg cgatacatcg ccgcttgcaa cggcttcgct
     2521 gaaagcgctc aggagccggg cgtgagaatc cggtgttgcc gcgggcgctg gccgtgtgtc
     2581 tcgcaccgca cgacgcgccc gcgtcgccag ttgccggcag gcggcctcgg tccggtcaag
     2641 catccgggca atctccgaga acggcacgtc gaagacgtcg tgaagcagga aggccgcgcg
     2701 ctccagcggc gacagccggt ccaccgccag cagcaacgcg aacgagaggt cgtcggcgag
     2761 ctccgtcgcg gcgtccgccg cgagcccctc cgcatcgaac accggctcgg gcagccaggg
     2821 gccgacgtag atttcgcgct gcgctttcgc actcttgagc cggtcgagac agagccgtgt
     2881 cacaacggtg accaggaagg cctcgggatt gtgaacgtcc tgcgcgccgg cgaagcggat
     2941 ataggcgtcc tgcaccacat cgtccgcgtc gctgcggctg ccgagcatgc gataggccag
     3001 cccaagcaag cgcccgcgat ggggcgccag cggatcgctt tcacgcggcc tgtctgatga
     3061 catcgaaatt gtgtccgtcc acgctgaccc gccggcactc cttggcgtag ccaagccctg
     3121 ccttcaccat tgggaacatc cggcccagtt gcagcgccag ggtcaagtcg atggccgcct
     3181 tcatcaggga tgcgaatttg aagaaagcgg acggcgcttc cttcagcatc atgtcaagat
     3241 aactggtgtc gtaaccgtag cgcttgctcg ttgcgcgcag cacccggtgc gcgatccaac
     3301 tacgcatgac gttctcctgg gtcagggaag gcggaaaaga gggcgagcgc cgacggcagt
     3361 acgacggcga tcaggtcgat cgcggtatga tgatcctgcc cggtcacgat cgccgccaga
     3421 tggatcagag cgtgagcggc caggaaagcc gagcctgcga ccgctgccgg ccaatacgcg
     3481 ggccgccagg cgcgcgccgc gagcgcaaga cccgcgacca ggaatgccgc gccaatgtcc
     3541 tggacaaaat ggggattata agggccggtt tcggacactc cgggcacgct cgcgtaccag
     3601 gttaggcccg caaacagcat catggagcca ttgagggttg tcagtgccgc caaaactccc
     3661 gcaatcgatc ttcgcatggg tatctcctgc gccctagacg agcgaggagg gtgcggtgtg
     3721 acagggcggg cgaaaagatt tcagggcgat cttcgcgggt tcgaaaaatg ccggaaatta
     3781 gcccttggca agcaggaaaa gactcgttaa aggatcgttt ccaggcgtgc tggctgtcgc
     3841 tggcacgtct gtgctcgcat tccgaaaacc cgatacgata ggagatcatt cctttcagga
     3901 catccgcatg gattgctcga aaggatgaga aaacccgtcg ttttggaggg atgtgggcag
     3961 gggtctttcg gctcgccgga cggcctcgat cctgtccaag actgcaggcg cctgcgacga
     4021 aacccaaagt ttcttcaacg gcttaatctc acggcctgca tagacgggtg aagaccggat
     4081 ttcgctttgc gtcgcgctcg ccgcgacgac tggcggatat ggttcgaacc tcgacacccc
     4141 gcgccttttg ggctcgggag ggcaggcttt tcaaatgtcg tcgtgcccga cgtgtcctga
     4201 gactggaatg tctcgaggtc aagttttggg cgggacgatg ggtgtaaccc ggcggtcctg
     4261 ccttttgggc aaagaccgcc aaccggagag agcttgctgt ggaaagagcg gcaaaaaagg
     4321 acgcggttga ggcgctcaat ggtcttttca agaccaccag cgttgcggtc gtggctcatt
     4381 attccggcct caccgtggct caaatgcaga agctgcgcat gcagatgaag caggcgggtg
     4441 cgtcggtgaa ggtctcgaag aaccgtcttg ccaaaattgc tcttgaaggc acggacgtcg
     4501 ttgccatcgg cgccctgttg aaggggccga ccgtgatcgc gacttcaaac gatccggtgg
     4561 cagcgccgaa ggttgccatc gaattcgcca agacgaacga aaacttcgtc attctcggcg
     4621 gatcgatggg gaaaaccgtc ctgaatgtcg atggcgtgaa ggcacttgcc gccctgccgt
     4681 cgctcgatga attgcgcgcg aagatcctcg gcctcctagt ggcgccggcg accaagatcg
     4741 ctcaactcac cactgcgccc gcagccaagc ttgcgcgcgt ggttcaggcc tatgcctcaa
     4801 agagcgaagc ggcctgacgc ccttcgctaa tcaaacctgg ttcgaaccga tacgcttaa
//
LOCUS       DATNOI010000530         4011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1829992,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000530 DATNOI010000000
VERSION     DATNOI010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829992"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1588
                     /gene="ppx"
                     /locus_tag="VN968_21470"
     CDS             86..1588
                     /gene="ppx"
                     /locus_tag="VN968_21470"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HXL31839.1"
                     /translation="MMRPRKRQSSVAVIDIGSNSVRLVVYESMTRSLVTLFNEKTLCG
                     LGREVQTTGLLAPDAVAKALTSLRRFRALCRVMKVARVYAIATAACRDATNGPDFIAK
                     AERICGVRIEILSGPREAKLSALGVISGIHKPDGIVGDLGGGSLELIDVQGNRVHSGV
                     TLPLGSLALQDLSHKSLKRAERIVRNDLSDVAQLKAGRGRTFYAVGGTWRALARIHIV
                     QSGYPLRVMHGYSIPAADALDFARRLRRLAAANMLADIEVVADARRPLLTYAALVLEY
                     IIRVAKPKTIVFSTFGVREGLLYSLLPQAERSEDGLICAAQTLNELLSRSARHAEELI
                     DWTDRLVRVVRLRETEEDRRLRHAACLLSDIGWRVHPDHRGEETLSLITNGNFGAATH
                     QGRVFVALSVFYRYAGLSEQNEPPAVIRELLPPAMVERARVLGAAFRVAHLVSAARPG
                     VLPATHFRSRSRKLMLVFEHRLADLVADRVGSRFKQLAKLVGRSGSIVRR"
     gene            complement(1626..2465)
                     /locus_tag="VN968_21475"
     CDS             complement(1626..2465)
                     /locus_tag="VN968_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HXL31840.1"
                     /translation="MNWLLQIFDPANIATLAVQFQAEMLQPAFWVAVGKIIWINVLLS
                     GDNALVIALACRGLAPRQRLWGMIFGAGAAVILRIIFTGIVASLMELPYLKLVGGLAL
                     LVIAAKLLVPEQEDEEGVESASHLWAAVQIVVVADIVMSLDNVIAVAAAANGSLPLLI
                     LGLAISIPLIVAGAALIMALLNRLPILVWAGAALLGWIAGDVIATDPAVHPKLQTLFA
                     GPAGVKLDAWLGPLGIGPHFASGNGGEVVCAVLGVIVVLIAGSIWRRRKLQQVEHAAP
                     SAG"
     gene            complement(2528..2950)
                     /gene="ndk"
                     /locus_tag="VN968_21480"
     CDS             complement(2528..2950)
                     /gene="ndk"
                     /locus_tag="VN968_21480"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXL31841.1"
                     /translation="MAIERTFSIIKPDATARNLTGAINALIEKAGLRIVAQKRIRMTR
                     EQAETFYAVHKERPFFRELVDFMISGPVVVQVLEGENAVLKHREVMGATDPSKADDGT
                     IRKLHAKSIGENSVHGSDAPETAAIEIAQFFSGNEIVG"
     gene            3231..3479
                     /locus_tag="VN968_21485"
     CDS             3231..3479
                     /locus_tag="VN968_21485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur globule protein precursor"
                     /protein_id="HXL31842.1"
                     /translation="MLRKLSLVAVAAASLGAAALAPTSASAWGGHGWHGAWHGGWGGP
                     RVFVGGPAYYGYGYGGCYVRRLVPTPWGPRWRLVNRCY"
     gene            3683..3874
                     /locus_tag="VN968_21490"
     CDS             3683..3874
                     /locus_tag="VN968_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31843.1"
                     /translation="MNSRITNDSEQIDHRTCRSICDAIGERLQQRLRPDTSRLSSHLQ
                     HLMDELRRRDSVGRWRSSN"
BASE COUNT          766 a         1246 c         1232 g          767 t
ORIGIN      
        1 ccgtggaaag tcgcttaagg aatcttcacc gcgccgcctc acgcgccgta ccgaacgtca
       61 gcattcatca taaaggattt ttccggtgat gcggccgcgc aaacgccaat ccagcgtcgc
      121 ggtcatcgac atcggttcga attcggtgcg tctcgtcgtc tacgaatcga tgacgcggag
      181 cctggtcacc cttttcaacg aaaaaaccct ctgcgggctc gggcgcgagg ttcagaccac
      241 gggactgctg gcgccggacg ccgtcgccaa ggccttgacg tcattgcggc gttttcgcgc
      301 cctgtgcagg gtgatgaaag tcgcccgggt ctatgcgatt gccaccgccg cgtgccgcga
      361 cgccaccaac ggccccgatt tcattgcgaa agccgaacgc atctgcggcg ttagaatcga
      421 aatcctgtcg ggtccgcgcg aagcgaaatt atccgcgctc ggcgtcatat cgggtattca
      481 caagccggac ggtatcgtcg gcgatctcgg cggcggctcg cttgaattga tcgacgtgca
      541 gggcaaccgc gttcacagcg gcgtgacgct gccgctcgga agcctcgcct tgcaggactt
      601 gtcccacaag tcgctgaaac gcgccgagcg catcgtcagg aacgatctgt cagacgttgc
      661 tcagctcaaa gccggacggg gacgcacctt ctatgcggtc ggaggaacct ggcgtgcgct
      721 ggcgcgtatc cacatcgtcc aaagcggtta tccgctgcgt gtgatgcacg gctattcgat
      781 accggcagcg gacgcgctcg atttcgcgcg ccgccttcgc cgcctcgcgg ccgcaaacat
      841 gctggccgat atcgaagtcg tcgccgatgc acggcggccg ctgctaacct atgcggcgct
      901 tgttctcgaa tacatcatcc gggtcgcaaa gccgaagacc atcgtgtttt caaccttcgg
      961 ggtgcgcgaa ggcctgcttt actcgctgct gccgcaggcc gagcgctcgg aggacgggtt
     1021 gatttgcgcg gcgcagacgc tcaacgagct gctgtcgcga tccgcacgcc atgcggaaga
     1081 attgatcgac tggaccgacc gtctggtgcg ggtcgtaaga ttgcgggaaa ccgaggaaga
     1141 tcgccgcctg cgtcatgccg cttgcctgct gtccgacatc ggatggcggg tgcatcccga
     1201 ccaccgcggc gaggagacgc tcagtctcat caccaacgga aatttcggtg cggccactca
     1261 tcaaggcagg gtgtttgtcg cattgtcggt attttatcgc tatgcaggct taagcgaaca
     1321 aaacgaaccg ccggcggtca ttcgtgagct gctgccgccg gctatggtcg agcgtgcccg
     1381 cgtgctcggc gcggcctttc gcgtcgccca cctggtatcg gcggcgcgac ccggcgtgct
     1441 gccggcaacc cactttagaa gccggagccg caagctgatg ctggtgttcg agcacaggct
     1501 ggccgatctg gtcgcggatc gcgtcggcag ccgattcaag cagctggcga aactggtcgg
     1561 ccgctccgga tcgatcgtaa ggcgttaatt tccgtcggca gcttgaagcc gaggctaggt
     1621 ccgagtcagc ctgcggacgg cgcagcgtgt tcaacttgct gcagcttgcg cctccgccag
     1681 atcgatcccg cgatcagcac gacgatgacg cccaacacgg cgcagacaac ctcgccgccg
     1741 ttgccgctcg caaagtgcgg cccgatcccg agcggcccca gccacgcgtc gagcttgacg
     1801 ccggccgggc cggcgaaaag agtctgcaat ttcggatgaa cggcagggtc ggtggcaatg
     1861 acgtcgccgg cgatccagcc gagcaatgcg gcgccggccc agaccaaaat cggcagccga
     1921 ttgagcagcg ccatgatcag cgcggcgccg gcgacgatca ggggtatgct gattgcaagg
     1981 ccgagaatca gcagcggaag gctgccattg gcggcggccg cgaccgcgat cacattgtcg
     2041 aggctcatta cgatatcggc gaccactacg atctgaaccg cggcccacaa atgcgacgca
     2101 gattcgacgc cttcctcgtc ctcttgctcg ggcaccagca gtttggcggc gatcacgagc
     2161 agtgccagcc cgccgaccag cttgaggtac ggcaattcca tcagcgacgc gacgataccg
     2221 gtgaaaatga tgcgaagaat aaccgcggcg cccgcaccga agatcatgcc ccaaagccgc
     2281 tggcgcggcg ccagtccacg acaggccagc gcgatcacca gcgcattgtc gcccgagagc
     2341 aacacgttga tccagatgat cttgccgacc gcaacccaaa aagccggctg cagcatctcg
     2401 gcttgaaact ggacggctaa cgttgcgatg ttcgccggat cgaagatttg cagcagccag
     2461 ttcacggcgc attcctttcg gccaggggcc gccccgcgcg gctattgctt gccgccgcgc
     2521 ctatcgatca gccgacgatt tcgttgccgg agaaaaattg tgcgatttcg atggcggcgg
     2581 tctcaggggc atcggagcca tgcaccgagt tttcgccgat cgacttggcg tgcagcttgc
     2641 ggatcgtgcc atcgtccgcc ttggaaggat cggtcgcgcc catcacttcg cggtgtttga
     2701 gcacggcgtt ttcgccctcg agaacttgaa cgacgacggg accggaaatc atgaaatcca
     2761 ccaactcgcg aaaaaacgga cgttccttgt gaacggcgta gaaggtttcg gcctgctcgc
     2821 gggtcatgcg aatgcgcttc tgggccacga tccgcagtcc cgccttttcg atcagcgcat
     2881 tgatcgcgcc ggtcagatta cgcgcggtcg cgtcgggctt gataatcgaa aaggtgcgtt
     2941 caatcgccat tatttcgtcc ttgcagggcg ctcaggggga attgcggcgc ttatatcggc
     3001 gccatcggcg aacggcaagc gatcagccga aacgcccctg cccagcaggc gatcgatgcc
     3061 cggaattacc acaatttcac aaagatgaac cctctctgaa accggcgtcc cggcttcatc
     3121 cacgttgtcg ggaatagtct tccaatcagc cgaacgtctc ggagttttgg cggcgttacg
     3181 gcggcacttt ccccgatggc gtcaaccgcg ccgaacctgt ggagacgacc atgttacgca
     3241 aactctcgct tgttgcagtt gccgcggcat cgctgggtgc ggccgcgttg gcgccgactt
     3301 ccgcctccgc ctggggcggt cacggttggc acggtgcatg gcacggcggc tggggtggtc
     3361 cacgcgtctt cgtcggtggc ccggcctatt atggctatgg ttacggcggc tgctacgtgc
     3421 ggcgattggt cccaacgccc tggggacctc gctggcggtt ggtgaatcgc tgctattgac
     3481 ctgcctcctc tccccgggcg tcaaaagccc ccggccgctg cggtcggggc ttttgtgcga
     3541 gttcgattag tggaactcgc ccagccgctt tcattcaatt ctagctggaa aacacctcca
     3601 accgaccgaa ggaggaaaca atttttgtgc tggggacttg atcggacgta gtcattgaaa
     3661 cggaacgcga agcggaaccg tcatgaacag ccgaatcacc aacgacagcg aacaaatcga
     3721 tcacaggacg tgtcgatcga tttgtgacgc catcggtgaa cggctgcagc agcgcctgcg
     3781 gccggatact tcgcggctat cgtcccactt gcagcatctg atggatgaac tgcgcaggag
     3841 ggacagcgta ggccgctgga ggtcatcgaa ctaaccgaat gtcttttcgg cataggcgga
     3901 ttgcggaagc gatatttcgc tcataaaccg gttgcgtttg gcggcggctc cggtgacaac
     3961 cccgcatgct ttcaatcacc gacatatcgg tccggattgc cggacgactt c
//
LOCUS       DATNOI010000531         4108 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1773980,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000531 DATNOI010000000
VERSION     DATNOI010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773980"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="VN968_21495"
     CDS             <1..117
                     /locus_tag="VN968_21495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HXL31844.1"
                     /translation="HRGEGWTPQTIAEHGVPALKSSFYKLDRSADIFNWDAI"
     gene            complement(268..3066)
                     /gene="ppc"
                     /locus_tag="VN968_21500"
     CDS             complement(268..3066)
                     /gene="ppc"
                     /locus_tag="VN968_21500"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HXL31845.1"
                     /translation="MSSQIMPSDADQKSNRAEEAQALEEDARLRNDIRLLGRILGDTV
                     RDQEGAGVFDLVERIRQTSIRFHRDNDRPARRELEIILDSMSITETVRIVRAFSYFSH
                     LANIAEDQNNIRQMRARASAGGSSRPGTLALTLSHARAAGLSAADLRRFFAGALVSPV
                     LTAHPTEVRRKSTIDREMEVAALLERRERVQLTPEELAADDEQLRREVLTLWQTNLLR
                     RTKLTVLDEVVNGLSFYDYTFLHEVPRLHCVLEDRLNEGAPATGGELASFLRMGSWIG
                     GDRDGNPFVTADVMRGTLRMQSSRVLRYYLEQLHVLGSELSLAAHLADVSEDLRALAG
                     RSPDTSPHRSGEPYRLAVSGIYARLTATAVRLDVETMRQPVGEAAAYAGAKELKADLD
                     ILHRSLIANNSGVIARGRLRLLRRAVDCFGFHLANLDMRQNSAVHERTVAELIDAAMP
                     GMSYLALNEEARISLLTNELRNTRPLASAFVKYSDETVGELAVFHAAAEAHIRFGAEV
                     IPQCIISMCEGVSDMLEVALLLKEVGLVNPSGRSAINIVPLFETIEDLQASAAIMDRM
                     LSIHDYRKLVDSRGLVQEVMLGYSDSNKDGGFVTSGWELYKAEIGLVEVFERHGVRLR
                     LFHGRGGSVGRGGGPSYDAIIAQPGGAVNGQIRITEQGEIISSKYSNAEVGRSNLEIL
                     AAATLEASLLQPKHSAPSGEYLKTMDQLSELAFKAYRGLVYETEGFVDYFWASTVITE
                     ISTLNIGSRPASRKKTRAIEDLRAIPWVFSWAQCRLMLPGWYGFGSAVEAWIAEHPDK
                     GMPFLKELYREWPFFRTLLSNMDMVLAKSSIAIASRYAELVPDTSLRENIFGRIRREW
                     HNSIETLLDIMGQQRLLQGNPLLERSIRNRFPYLDPLNHVQVELLKEHRAQNPDEQVL
                     RGIQLTINGISAGLRNSG"
     gene            complement(3272..4084)
                     /locus_tag="VN968_21505"
     CDS             complement(3272..4084)
                     /locus_tag="VN968_21505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXL31846.1"
                     /translation="MEQRVSVAIADGVADVRLVRADKMNALDAAMFDALVATSERLAH
                     EKGVRAVVLSGEGRSFCAGLDMGRFAAMKEKGGNGVAGGEDRDLTRRTHGLANFPQQA
                     VWGWRQLPVPVIAAIQGVAFGGGFQLALGADMRFLSPDARMSIMEIKWGLVPDMAGTP
                     ILASLVRDDILRELTYTGRIFSAQEALSYGLATRICDDPRATAFEVAREIAGKSPDAI
                     RAAKRLLNNLSVNPGPALSAESIEQMKLIGGANQLEAVRANIDKRPPRFADV"
BASE COUNT          750 a         1335 c         1271 g          752 t
ORIGIN      
        1 catcgcggcg aaggctggac gccccagacc atcgccgaac acggcgtgcc cgcgctgaag
       61 tcgtcgttct acaagctcga ccgctcggcg gatattttca attgggatgc gatctagctc
      121 atcgtcgtcc ctgcgaaagc aatgcgaaag cagggaccca taaccaccga ccttaattgt
      181 tagaaagccg tctactccca gcgcaaaatc acaagggcac ggcgtatggg tccccgcttt
      241 cgcgtcgctt tcgcggggac gacggcctca cccgctattg cgcagccccg ccgaaattcc
      301 gttgatggtg agctggatgc cgcgcagcac ctgttcatcg ggattctgcg cgcgatgttc
      361 tttcaacagt tccacctgca catggttcag cggatcgaga tagggaaagc ggttgcggat
      421 cgagcgctcc agcagcgggt tgccctgcag caggcgctgc tgccccatga tgtccagcaa
      481 cgtctcgatc gaattgtgcc attcgcgccg gatgcgcccg aagatatttt cgcgcaaact
      541 cgtatccggc accagttcgg catagcgcga ggcgatcgcg atcgagcttt tggccagcac
      601 catgtccata ttcgacagca aagtgcggaa gaacggccat tcgcgataaa gctctttgag
      661 gaacggcatg cccttgtcgg gatgctccgc gatccatgcc tccaccgcgc tgccaaagcc
      721 gtaccacccc ggcagcatca gccggcattg cgcccagctg aacacccagg gtatggcgcg
      781 aaggtcctcg atcgcgcggg tttttttgcg cgacgccggg cggctgccga tattgagcgt
      841 cgagatttcg gtgatgaccg tcgaggccca gaaatagtcg acaaaaccct cggtctcgta
      901 aaccagtccg cgataggcct tgaaagcgag ctccgacaac tggtccatcg tcttcagata
      961 ttctcccgat ggagcgctgt gctttggctg aagcaggctg gcttcgagtg tcgctgcggc
     1021 gagaatttcc aggttgctgc ggccgacctc ggcattggaa tatttgctgg agatgatttc
     1081 accctgctcg gtgatccgga tctggccgtt caccgcgccg ccgggttgcg cgatgatggc
     1141 gtcgtagctc ggcccgccgc cgcggccgac cgagccgccg cggccgtgaa acagccgcaa
     1201 ccgcaccccg tgacgctcga acacctcgac caggccgatc tcggccttgt agagctccca
     1261 gcccgaggtg acgaaaccgc cgtccttgtt gctgtcggaa taaccgagca tcacctcctg
     1321 caccaagccg cggctgtcca ccagcttgcg atagtcgtga atcgacagca tccggtccat
     1381 gatcgcggcg gacgcctgca aatcctcgat ggtctcgaac agcggcacga tgttgatcgc
     1441 gctgcggccg gagggattga ccaggccgac ctctttcaag agcagcgcga cctccagcat
     1501 gtcggacacc ccctcgcaca tcgagatgat gcattggggg atcacttccg cgccgaaccg
     1561 gatatgcgcc tcggcagccg catggaagac cgccagctca ccaaccgtct cgtccgagta
     1621 tttcacgaag gcggaagcga gcggccgcgt gttgcgcaat tcgttggtca gaagcgagat
     1681 ccgcgcctct tcgtttagcg ccagatagga catgccgggc atggccgcgt cgatcagctc
     1741 ggccacggtg cgttcatgca ccgcggagtt ttgccgcatg tcgagatttg ccaggtgaaa
     1801 accgaaacag tccaccgcgc ggcgcaacag gcgcagcctg ccccgcgcga tcacgccgga
     1861 attattcgcg atcagcgagc ggtggagaat gtcgaggtcg gccttgagct ctttcgcacc
     1921 ggcataagcg gccgcttctc ccaccggctg cctcatggtt tccacatcca gcctgacagc
     1981 ggtcgcggtg agccgggcgt aaatgccgga caccgccagc cgataaggct cgccgcttcg
     2041 atgcggcgaa gtgtcgggcg agcgccccgc cagcgcgcgc agatcttcgg agacgtcggc
     2101 gaggtgcgcc gcgagcgaca attcggagcc gagcacgtgc agttgttcca gatagtagcg
     2161 cagcacacgg ctcgactgca tgcgaagcgt gccacgcatc acgtctgccg tgacgaacgg
     2221 attgccgtcg cggtcgccgc cgatccagct tcccatcctc aggaacgacg ccagctctcc
     2281 gcccgtggcg ggggcgccct cgttcaatcg gtcctccagc acgcaatgca gccgcggcac
     2341 ctcatgcagg aaggtgtagt cgtaaaacga gaggccgttg acgacctcgt cgagcacggt
     2401 gagcttggta cggcgcagca gattggtctg ccatagcgtc agcacctcgc ggcgcaactg
     2461 ctcgtcatcg gccgcgagtt cttccggcgt gagctggacg cgttcgcggc gctccaacag
     2521 cgcggcgacc tccatctcgc ggtcgatggt gctcttgcgg cgaacctcgg tcgggtgcgc
     2581 ggtcagaacc ggactgacca gagctcccgc aaaaaaacgg cgcagatcgg ccgcgctgag
     2641 gccggccgca cgtgcgtgcg acagcgtcag cgcaagggtg cccgggcgcg acgatccgcc
     2701 tgcactagcg cgcgcccgca tctggcggat gttgttctgg tcttcggcga tgttggcaag
     2761 gtgcgagaag taactgaagg cgcgcacgat gcgcacggtt tcggtgatcg acatgctgtc
     2821 gaggatgatt tcgagctcgc gccgcgccgg cctgtcgttg tcccggtgga accggatcga
     2881 ggtctgccgg atgcgttcga ccagatcgaa cacgccggcg ccttcctgat cgcgcacggt
     2941 atcgccgagg atgcgcccga gcagccggat gtcgttgcgc agccgcgcgt cttcttcaag
     3001 cgcctgggct tcctcggcgc gattggactt ttggtcggcg tcggaaggca tgatctggga
     3061 cgacatggct ggctgctcca gaaactgccc ggcagtctct tgagtcgcaa tttttcaccc
     3121 gcaacgcaag tccaagaaga aggcggctcg tcgggtcgat gagccctcat ggtgaggagc
     3181 gcatcctcgc gcgtctcgaa ccatgaggat gagatgtcat ccttcgagac gcggccaagc
     3241 ggccgctcct caggatgagg ccgcgcgttg atcaaacatc agcaaagcgc ggcgggcgct
     3301 tgtcgatgtt ggcgcggacc gcttcgagct gattggcgcc gcctatcaat ttcatctgct
     3361 cgatggattc ggctgatagc gccgggccgg ggttgacgga aagattgttc agcaggcgct
     3421 tggcggcacg gatggcgtcc ggactcttgc cggcgatctc gcgcgcgacc tcaaacgccg
     3481 tggcgcgcgg gtcgtcgcag atccgcgtcg cgagaccgta actcagcgct tcctgcgccg
     3541 agaaaatccg cccggtatag gtcagctcac gcaggatatc gtcgcggacg aggctcgcca
     3601 ggatcggcgt ccccgccatg tccggcacca ggccccattt gatttccatg atcgacatcc
     3661 gggcatcggg gctgaggaag cgcatatcgg cccccagcgc gagctgaaag ccgccgccga
     3721 acgccacgcc ctggatcgcg gcgatcaccg gcaccggaag ctgccgccat ccccataccg
     3781 cctgctgcgg aaaattggct aggccatggg tgcgcctggt caggtcgcgg tcttcgcccc
     3841 cggctacccc gttaccgccc ttctccttca tggcggcgaa ccggcccata tcgaggccgg
     3901 cgcaaaacga gcggccctca ccggacaata ctaccgcccg cacgcctttt tcatgggcga
     3961 gccgctcgga agtggcgacc agtgcatcga acatcgcggc atccagcgca ttcatcttgt
     4021 ccgcccgcac caaacggaca tcggcgacgc cgtccgcaat tgctaccgag acgcgctgct
     4081 ccatggatgg ttctccctgc tgtttttt
//
LOCUS       DATNOI010000532        11624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_53707,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000532 DATNOI010000000
VERSION     DATNOI010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11624
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53707"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1243
                     /locus_tag="VN968_21510"
     CDS             29..1243
                     /locus_tag="VN968_21510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXL31847.1"
                     /translation="MSVRPQTKDKPSPVNFQWDDPFMLDEQLTEDERMIRDTARAYAQ
                     DKLLPRVTKAYLEEKVDRDIFHEMGELGLIGITLPEEYGCANASYVAYGLVAREIERV
                     DSGYRSMNSVQSSLVMHPIYAYGDENQRKKYLPKLATGEWVGCFGLTEPDAGSDPGGM
                     KTRAEKTSDGYRLTGTKMWISNAPIADVFVVWAKSAEHNNQIRGFILEKGMKGLSAPK
                     IGGKLSLRASVTGEIVMEGVEVPETALLPNVSGLKGPFGCLNRARYGISWGAMGAAED
                     CMARARQYTLDRKQFNRPLAATQLVQKKLADMQTDIALGLQASLRVGRLMDEGKMAPE
                     MISIVKRNNCGKALDIARMSRDMHGGNGIQIEYHVMRHAQNLETVNTYEGTHDVHALI
                     LGRAITGIQAFS"
     gene            1374..2300
                     /locus_tag="VN968_21515"
     CDS             1374..2300
                     /locus_tag="VN968_21515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL31848.1"
                     /translation="MSDNDDIPFNRDFPLKPGVADEARPGVRRILCNNSSPFTFTGTV
                     SYIVGKGNVAIIDPGPDDEAHAAALLDAVRHETVTHILVTHTHRDHSPNTARIKAATG
                     ATVYAEGPHRASRPRFESEKHNPESGADRDFRPDVQVKNGDMIEGSCWTLETVATPGH
                     TANHLAFAWPERKINFVGDHVMGWSTSIVAPPDGSMIDYMASLDRLTARDEDLYFSGH
                     GPEIPDAPRYVRFLIRHRQAREASILHRLAKGETDIPSMVRAIYIGIDPRLTGAAGYS
                     VLAHLEDLVARGIVATDGDPVIGGSYRLASSD"
     gene            complement(2431..3279)
                     /locus_tag="VN968_21520"
     CDS             complement(2431..3279)
                     /locus_tag="VN968_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HXL31849.1"
                     /translation="MARRFSAPYQSEPVSSLASWARNLAVFSVVAVLVSILIVRFGFL
                     EIKPALATFFGALACAGLSILVGLAAFVAIWQNGSRGMSRILLALLIDAVVLAYPAYL
                     GLQYRKLPQIHDITTDPIDPPRFDALAALRTGDGANTAVYAGLYSAEQQRHYYPDIET
                     VELDVPVQRAYEVALQLVNRRKWLVIDERAPQLPRRIGRIEAVARTPIMGFREDVSIR
                     ATPDGEGSRVDIRSSSRYFESDLGSNAARITRLIDDINNAVDNAKPVVKKPVVPAKVP
                     PKVVKK"
     gene            complement(3493..3768)
                     /locus_tag="VN968_21525"
     CDS             complement(3493..3768)
                     /locus_tag="VN968_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HXL31850.1"
                     /translation="MRGTIRRTIRSRKLRVDQTEAETTLWNRIRNRQLSAYKFVRQEP
                     IGPYICDFVYRAELVIIEVDGGQHSASTRDQARDRYLCAQGYRVMRF"
     gene            complement(3842..5470)
                     /locus_tag="VN968_21530"
     CDS             complement(3842..5470)
                     /locus_tag="VN968_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid--CoA ligase"
                     /protein_id="HXL31851.1"
                     /translation="MLGLMQDWPLLCHRIIEHAAAVHSTQEVVTRSVEGPIHRTNYAE
                     IRDRALKVSQRFDRDGIKLGDRVATIAWNTWRHLEAWYGIMGIGAICHTINPRLFPDQ
                     IAWIVNHAEDRVVMADITFVPILEKIADKLPSVERYIVLTDGAHMPKTTLKNAVAYED
                     WIAEADGDFKWKTFDENTAAAMCYTSGTTGDPKGVLYSHRSNVLHALMANTGDSLGAN
                     AADTMLPVVPLFHANSWGIAFSAPSMGTKLVLPGAKLDGASVFELLDTEKVTHTAGVP
                     TVWLMLLNHIAANHLKLPNLRMVVCGGSAMPRSMIKSFLDMGVQVRHAWGMTEMSPIG
                     TLASLKPPFSELTGEARLDILQTQGYPPFGVRMKITDDAGRTLPWDGKTFGRLKVSGP
                     AVSKGYFRVDADILDEEGYFDTGDVATIDANGYMRITDRSKDVIKSGGEWISSIDLEN
                     LAVGHPAIAEAAVIGIHHPKWDERPLLIVQLKQGQTATREDILKFMEGKIAKWWMPDD
                     IAFVDGIPHTATGKILKTALRDQFKAYSFPNAAA"
     gene            5624..6766
                     /locus_tag="VN968_21535"
     CDS             5624..6766
                     /locus_tag="VN968_21535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HXL31852.1"
                     /translation="MLGTQTESAAARRSRSWFGAPFSDLFGQARRKPRRAAHSTPTPA
                     PALAAVPLPKSRPADAPVTEPEKPSAAKPAPVATDRLAEHATPPLPKPRPADAPVTEP
                     EKPAAAKPVPATTDKPAEQASPPPPPSACRLALTDAIAIAPSIPDIKGPGECGGEDLV
                     RLEAVVLPDKRRVSVKPAAILRCAMATEIANWIRIDMAPLATSLGSVISDLNNLDSFE
                     CRGRNGVAGAKMSEHGRANALDVHDLKLASGQSISFTDRTVPREVRETVLHSMCARFT
                     TVLGPGSDGYHEEHIHLDLMERHNNYKICQWNVWDPLPQIAPLLPAERPEEAPPRELA
                     AKSDAVKSDAAKPDAAKADAAKSDASEPATGATKSPTKKRRENRRL"
     gene            complement(6797..7474)
                     /locus_tag="VN968_21540"
     CDS             complement(6797..7474)
                     /locus_tag="VN968_21540"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXL31853.1"
                     /translation="MSSLYVKLGILAAGLVLSGCMQATTYEATNQTVFKPRDKELLAK
                     VSYVKTPVPEAFRRAIVDYHRKEAPGSIVVDSDNHYLYLVQDGGKAIRYGITVGEEAM
                     AWSGIAKIGSMTEWPPWHPTPGEISRLGVPKFVAPGPDNPMGSRALYLYSGGKDTLFR
                     IHGTNQPEYIGASISSGCIRMTNEDVIDLYNRVKMGTIVVVLEPKHGDSPFNSQMALQ
                     GGGNSIQ"
     gene            7807..8787
                     /locus_tag="VN968_21545"
     CDS             7807..8787
                     /locus_tag="VN968_21545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HXL31854.1"
                     /translation="MLSMFVPSEASLKKVVCADLAALPESAVWIDLVKPTAAEDHAVE
                     RLAGIAVPTREDMQEIEISSRLYIENGVRYMTATLMCAADTQNPRTTAVTFILAAHRL
                     VTVRYDEPKPFMLVENKLARACAPGVTGEMVLMELLDAVIDRNADILERAGGDMDNIS
                     HDIFEPEGAARTGHSKRYSDILIAIGRKGDLTSKVRESLVSIGRVVTFVAAGTEGVKW
                     SKDMREQLKIMQRDVTSLTDHASYLSNKITFVLDAMLGVVNLEQNNIIKLFSVMAVVL
                     MPPTLIASIYGMNFKSMPELEWTHGYPYALVAMLLAAVGPYLFFTWKKWL"
     gene            9070..9294
                     /locus_tag="VN968_21550"
     CDS             9070..9294
                     /locus_tag="VN968_21550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31855.1"
                     /translation="MVEKVITASENVVDFARYQVDRKAGKALAISARACRHCGAVLSE
                     GESEDECSSAFNIDNARLRWQAAQILRGLI"
     gene            complement(9515..10267)
                     /locus_tag="VN968_21555"
     CDS             complement(9515..10267)
                     /locus_tag="VN968_21555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL31856.1"
                     /translation="MPSSTNAPRRTLLLTGASRGIGHATVIRFSSAGWRVITCSRHPF
                     PEECPWGAGPEDHIQVDLADHADTTRAISEIRERLEGGDLHALVNNAAISPKAAGGGR
                     LGSIDTDIDTWSHVFRVNFFAPIMMARGLIEELKAAKGSVVNVTSIAGSRVHPFAGAA
                     YATSKAALASLTREMASDFGRIGVRVNSIAPGEIDTSILSPGTERIVEQQIPLHRLGT
                     PDEVAKIIYVLCTETSSYVNGAEIHINGGQHV"
     gene            complement(10291..10932)
                     /gene="pdxH"
                     /locus_tag="VN968_21560"
     CDS             complement(10291..10932)
                     /gene="pdxH"
                     /locus_tag="VN968_21560"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HXL31857.1"
                     /translation="MTDTTSIKHPTPLTSGDFTAAEEPFALFGEWFAEAVKAEPNDPN
                     AMSLATVDADGLPDVRMVLMKGYDASGFVFYSHIASQKGRELATNPKAALLFHWKSLR
                     RQIRIRGNVSPVTDAEADAYFATRPKQAQIGAWASKQSQPLESRFAFEQAIAKIAARH
                     LVGEVPRPPGWSGWRIAPLQFEFWHDRPFRLHDRIEFRRATPDQPWSRTRLYP"
     gene            11055..11462
                     /locus_tag="VN968_21565"
     CDS             11055..11462
                     /locus_tag="VN968_21565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HXL31858.1"
                     /translation="MILILIGAGSGGCSLSRTDGAFARMEDHGVTGSVGLAQAGGLMP
                     TESDLAFARNAASDVLTKGTKDSSQPWENPETGARGSVTPLAEAYASDGRTCRDFLAS
                     YVNGRSESWLQGAACQSEQGRWEIHTLKPWRRG"
BASE COUNT         2212 a         3596 c         3680 g         2136 t
ORIGIN      
        1 gtgggatagc tatgcgaaag gaattttcat gagcgtgcgc cctcagacca aggacaagcc
       61 gtcgccagtg aattttcagt gggacgatcc gttcatgctc gacgagcagc tcaccgaaga
      121 cgagcgcatg atccgcgaca ccgcgcgcgc ctatgcgcag gacaagttgc tgccgcgcgt
      181 caccaaggcc tacctggaag aaaaagtcga tcgcgacatc tttcacgaga tgggcgaact
      241 cggcctgatc ggcatcacgc taccggaaga atacggctgc gccaatgcga gctatgtcgc
      301 ctatggcctc gtggcgcgcg agatcgagcg tgtcgattcc ggttatcgct cgatgaattc
      361 ggtgcagtcg tcgctggtga tgcacccgat ctatgcctat ggcgacgaga accagcgcaa
      421 gaaatatctg cccaagctcg cgaccggaga atgggtcggt tgtttcggcc tgaccgagcc
      481 ggacgccggt tccgaccccg gcgggatgaa gacccgggcc gagaagacgt ccgacggcta
      541 ccggctgacc ggcaccaaga tgtggatttc caacgcgccg atcgccgacg tattcgtggt
      601 gtgggcgaaa tcggccgagc acaacaacca gatccgcggt tttattctcg aaaagggcat
      661 gaagggcctg tcggcaccga agatcggcgg caaactttcg cttcgcgcct ccgtcaccgg
      721 cgagatcgtg atggaaggcg tcgaggtgcc ggaaacagcg ctcttgccca acgtgtccgg
      781 cctgaaggga ccgttcggct gcctcaaccg ggcccgttac gggatttcct ggggcgcgat
      841 gggcgcagcc gaagattgca tggcgcgcgc gcggcaatat acgctcgacc gcaagcagtt
      901 caatcggccg ttggcggcga cgcaactggt gcagaagaag ctggcggaca tgcagaccga
      961 catcgcactg ggcttgcagg cctcgttgcg ggtggggcga ttgatggacg agggcaagat
     1021 ggcgccggag atgatttcga tcgtcaagcg caacaattgc ggcaaggcgc tcgacatcgc
     1081 gcggatgtcc cgcgacatgc atggcggcaa cggcatccag atcgaatacc acgtgatgcg
     1141 gcatgcacag aacctcgaga ccgtgaatac ctatgagggc acccacgacg tccacgccct
     1201 cattctcggc cgtgccatca ccggcattca ggcgttttcg tagagcgcat cacgtcgact
     1261 ccgtaggatg ggttgagcgc agcgaaaccc atcgtcatag ctccctatgc gttgcgtgat
     1321 gggtatcgct gcgctcaacc catcctacga gatcgcaaca caggcgtacc cccatgtctg
     1381 acaacgacga cattccgttc aaccgcgact ttccgctgaa gcctggcgtg gcggatgaag
     1441 cccgccccgg cgtgcggcgg atcctctgca acaattcgag ccctttcacc ttcaccggta
     1501 cggtgagtta cattgtgggc aagggcaacg tcgcgatcat cgaccccggc cccgacgatg
     1561 aggcccatgc cgccgcgctg ttggacgccg tgcgccatga gacggtaacc catatcctgg
     1621 tcactcatac ccatcgcgac cattcgccga acaccgcgcg gatcaaggcg gccaccggcg
     1681 ccaccgtcta tgccgagggt ccgcatcgcg cctcgcggcc gcgcttcgag agcgaaaaac
     1741 acaatccgga atcgggtgcc gaccgcgact tcaggccgga cgttcaagtg aaaaacggcg
     1801 acatgatcga gggatcctgc tggacgctgg aaacggtggc cacgcccggc cataccgcca
     1861 accatctggc gttcgcctgg ccggagcgaa aaatcaattt cgtgggtgat catgtgatgg
     1921 gctggtcgac ctcgatcgtg gcgccgccgg acggatcgat gatcgactac atggcctcac
     1981 tcgaccggct gacggcgcgc gatgaggatc tgtatttctc cggccacgga ccggaaatcc
     2041 cggacgcgcc gcgttatgtg cgcttcctga tccggcaccg tcaggcccgc gaagcctcga
     2101 tcctgcatcg cctcgccaag ggcgagacgg atattccttc catggtgcgc gcgatctata
     2161 tcggcatcga cccgcggctg acgggtgcgg ccggctattc cgtgcttgcg catctggaag
     2221 atctggtggc gcgcggcatc gttgcgaccg atggcgaccc ggtgatcggc gggagctatc
     2281 gattggcgag tagcgattag ggagtaggaa gcggcgaata gcgagtggcg aatagcgaat
     2341 agaaaaagcg gagcagcgcg aacccaatct tctttcctat tcgccactcc ctattcgcca
     2401 ttcgctactc gccattcgcc acttcgccac tcacttcttc acgaccttcg gcgggacctt
     2461 ggccggcaca accggcttct tcaccaccgg tttggcgttg tcgaccgcgt tgttgatgtc
     2521 gtcgatcagc ctggtgatgc gcgcggcgtt gctgccgaga tcgctctcga aatagcgtga
     2581 cgaggagcgg atatcgaccc gcgatccctc gccgtcgggc gtggcgcgga tcgacacgtc
     2641 ctcgcggaat cccatgatcg gcgtccgcgc caccgcctcg atgcggccga tgcggcgcgg
     2701 cagctgcggg gcgcgttcgt cgatgaccag ccatttgcgc ctgttgacca gctgcagtgc
     2761 gacctcatag gcgcgttgca cggggacatc gagttcaacc gtctcgatat cgggatagta
     2821 atgccgctgc tgttcggcgg aatacagtcc ggcatagacg gcggtattgg cgccatcgcc
     2881 ggtgcgcaag gccgccagcg cgtcaaaacg cggcggatcg atcggatcgg tggtgatgtc
     2941 gtggatctgc ggcagcttgc gatattgcag gccgagatag gcggggtagg cgaggacgac
     3001 cgcgtcgatc aacagcgcca gcaggatgcg gctcatgccg cgcgatccgt tctgccagat
     3061 cgcgacaaag gcggccagcc cgaccaggat cgaaagcccg gcacaggcca gcgcgccaaa
     3121 gaacgtcgcc agcgccggtt tgatttcaag gaagccgaag cgaacgatga ggatcgagac
     3181 cagcaccgcg accaccgaga acaccgcgag attgcgcgcc cacgacgcga ggctggacac
     3241 gggctccgac tgatagggag cggaaaacct gcgggccatc gcttcaattc tgccgggttg
     3301 gaaccgtgcc cggcgagata ctgccgggct gcactctggc ccgtttgaga ccacggacct
     3361 gcggcaaatt caaggggtgg attgggggtg gggtgttctt aaacctctcc ccgctctgcg
     3421 cggggagagg tcggactgcg gagcagtccg ggtgagggtc aaggcactgc gcctgcaacg
     3481 acgattaaac tatcagaagc gcatcacacg ataaccctgc gcacacaagt agcgatctcg
     3541 cgcctggtcg cgtgtcgacg ctgaatgctg cccgccatcg acttcgatga tcaccaactc
     3601 agcccggtaa acgaagtcgc agatataggg gcctatgggc tcctgtctga cgaacttata
     3661 tgcactgagc tgccggttac gaatgcgatt ccagagcgtc gtttcggctt cggtctggtc
     3721 tacgcgtagt tttcgcgacc ggatggttct acgaatggtg ccgcgcattg cctcgcccct
     3781 cacccggatc gcaagggcga tccgacctct ccccacgcag agtggggaga ggttagtacg
     3841 ctcacgccgc tgcgttggga aaactgtacg ccttgaactg gtcgcgcagc gcggttttga
     3901 gaatcttgcc ggtcgcggtg tggggaatgc catcgacgaa cgcgatgtcg tcgggcatcc
     3961 accatttggc gatcttgcct tccatgaatt tcaggatgtc ctcgcgcgtc gcggtctggc
     4021 cctgcttgag ctgcacgatc aaaagcggac gctcgtccca tttgggatgg tgtatgccga
     4081 tcacggccgc ctccgcaatc gcgggatgtc cgaccgcgag gttttccaga tcgatcgacg
     4141 aaatccattc gccgccggat ttgatcacat ccttggaacg atcggtaatc cgcatgtacc
     4201 cgttggcgtc gatggtcgcg acgtcgccgg tgtcgaaata gccttcctcg tcgaggatat
     4261 cggcatcgac gcggaaatag cccttcgata ccgcgggacc gctgactttc agccggccga
     4321 acgtcttgcc gtcccatggc agggtcctgc cggcgtcgtc ggtgatcttc atccgcacgc
     4381 cgaagggcgg atagccctgg gtctggagga tgtcgagccg ggcctcgccg gtgagttcgc
     4441 tgaacggagg tttcagcgag gcaagggtgc cgatcgggct catctcggtc atgccccagg
     4501 cgtggcgcac ctgcacgccc atgtcgagga acgacttgat catcgagcgc ggcatcgccg
     4561 agccgccgca aaccaccatc cgcagatttg gcagtttcag atggttggcg gcaatatgat
     4621 tgagcagcat cagccacacc gtcggaacgc cggcggtatg cgtcaccttt tcggtatcca
     4681 acagctcgaa caccgacgcg ccatcaagtt tcgcgccggg gagcaccagt ttggtgccca
     4741 tcgacggcgc cgagaacgcg ataccccagc tgttggcatg gaacagcgga accaccggca
     4801 gcattgtatc tgcggcattc gcaccgaggg aatcgccggt gttggccatc aaggcgtgga
     4861 gcacgttgga acgatgcgaa tacaacacac ctttgggatc gcccgtggtg ccggaggtgt
     4921 agcacattgc ggctgcggta ttttcgtcaa acgtcttcca cttgaaatcc ccgtccgcct
     4981 ccgcgatcca gtcctcatag gcgaccgcgt tcttcagcgt ggttttcggc atgtgcgccc
     5041 cgtcggtgag cacgatgtag cgttccacgc tgggcagctt gtcggcgatt ttttccagga
     5101 tcggcacaaa ggtgatatcg gccatcacca cacggtcttc cgcgtgattg acgatccagg
     5161 cgatctggtc ggggaacagc cgcggattga tggtgtggca gatcgccccg atgcccatga
     5221 tgccgtacca ggcttcgaga tgccgccagg tgttccaggc gatggtggcg acacggtcgc
     5281 cgagcttgat gccgtcgcga tcgaaacgct gcgagacctt cagggcgcgg tcccggattt
     5341 cggcgtaatt ggtgcgatga atggggcctt cgaccgatcg cgttaccact tcctgcgtgc
     5401 tgtgaaccgc cgctgcgtgc tcgataatcc ggtggcaaag caaaggccag tcttgcatca
     5461 gtccaagcat gcgaacgttc ctcccagagt gttatcactt gccgcaggca ttcacggaat
     5521 tgacatgaac tctagcgcgc ggaaagcgag ccgcaaatgc ccttatagcg gcccgggcag
     5581 cttcacggcg gtaatattgg ttactgccgt aactgcactg gcgatgcttg gcacacaaac
     5641 cgagtctgcc gcggcgcgaa ggtcgcgcag ctggtttggt gcccccttta gcgacctgtt
     5701 cggacaggcg aggcggaagc cgcggcgcgc tgcgcattcc acacccacac ccgcgcccgc
     5761 actcgcagcc gtaccgttgc cgaagtcgcg tccggccgat gcgccggtaa ccgaaccgga
     5821 aaagccctcg gccgcaaaac cggcgcccgt agcgactgac aggctggccg aacatgccac
     5881 gccaccgttg ccgaagccgc gtccggccga tgccccggtg accgagccgg aaaagccggc
     5941 ggccgcaaaa cccgtgcccg ccacgaccga caagccagcc gaacaggcct cgcccccgcc
     6001 accgccgtcg gcctgccggc tggccctgac cgatgcgatc gccattgccc ccagcatccc
     6061 cgacatcaag ggtcccggcg aatgcggtgg ggaggatctg gtgcggctgg aggccgtggt
     6121 gctgccggac aagcggcggg tttcggtaaa acccgccgcg atcctgcgct gcgccatggc
     6181 caccgagatc gcgaactgga tccgcatcga catggcgccg ctggcgacga gccttggcag
     6241 cgtcatcagc gatctcaaca atctcgattc gttcgaatgc cgcggacgca acggtgtcgc
     6301 cggcgccaaa atgtccgagc acggccgcgc caatgcgctc gacgttcacg acctgaaact
     6361 cgccagcggc caatcgatct cattcaccga tcgcacggtg ccgcgcgagg ttcgcgagac
     6421 cgtgctgcat tcgatgtgcg cgcggtttac caccgtgctg gggccgggtt cggatggtta
     6481 ccacgaggag cacatccatc tcgatctgat ggagcggcac aacaactaca agatctgcca
     6541 gtggaacgtg tgggacccgc tgccgcagat cgcgccgctg ctgccggccg agcggccgga
     6601 ggaagcgccg ccgcgcgagc tcgccgcaaa atccgacgcg gtcaaatccg atgccgccaa
     6661 accggatgca gctaaagctg acgcggcgaa atcggatgcg tccgagccgg ccaccggtgc
     6721 gaccaagtcg ccaacaaaaa agcgccggga aaaccggcgc ctttgatctc gtctctacgg
     6781 tcgccgcgag aagcggctac tggatcgagt tgccgccgcc ctgcagcgcc atctgcgaat
     6841 tgaacggcga gtcgccgtgc ttcggttcca gcacgaccac gatggtaccc atcttcaccc
     6901 ggttatacag gtcgatgacg tcctcgttgg tcatccggat gcagccggag gagatcgacg
     6961 cgccgatata ttccggctgg ttggtgccgt gaatccggaa caaggtatcc ttgccgccgg
     7021 agtacagata cagcgcgcgc gaacccatcg gattgtccgg accgggtgcc acgaacttcg
     7081 gcacgccgag ccgcgagatt tcgccgggcg tcggatgcca gggcggccac tcggtcatgc
     7141 tgccgatctt ggcaatgccg gaccaggcca tcgcttcttc accgacggtg atgccatagc
     7201 ggatcgcctt accgccgtcc tgcaccagat agaggtaatg attgtcggaa tcgaccacga
     7261 tcgagcccgg cgcttccttg cggtgatagt cgacgatggc gcggcggaac gcttccggaa
     7321 ccggcgtctt gacgtaagag accttggcca ggagttcctt gtcgcgcggc ttgaaaacgg
     7381 tctggttggt cgcttcatag gtggtggcct gcatgcagcc cgacagcacc aggccggcgg
     7441 ccagaattcc cagttttacg tacagcgacg acattgtgca cttccagtcg aaagcctcgc
     7501 gttccaggca ccagcatgga gcctaaacgc cacgattccg ttaagcctat tatcgttaaa
     7561 accggtcaca attccagagt ttagattctc atcccgcgtt gcggcacccg gttgtgtctt
     7621 ttttgtcgca agttccgcgg tttgcggctg atttttgggc aggtgcgccc gtaccgccgg
     7681 accagcacca catgatcgcc accgagccgc cgcaaatggg gctagacacc ggttctgtca
     7741 tgagcgcttg ccagaatcga gctaagtcct gttgggtggt tggcatgctc agttccggag
     7801 tccttgatgt tatcgatgtt cgtcccctcc gaagcctcgc tgaagaaagt cgtttgcgcc
     7861 gacctcgccg cgctgccgga gagcgcggtc tggattgatc tggtgaagcc gaccgcggcg
     7921 gaggaccatg ccgtggagcg gctcgccggc atcgcggtac cgacgcggga ggacatgcag
     7981 gagatcgaga tttccagccg gctctatatc gagaacggcg tccgctacat gacagccacg
     8041 ctgatgtgcg cggccgacac ccagaatccg cgcaccaccg cggtgacctt catcctcgcc
     8101 gcccatcgcc tggtgacggt gcgctatgac gagccaaagc cgttcatgct ggtggagaac
     8161 aagctggcgc gcgcatgcgc gcccggcgtc accggcgaaa tggtgctgat ggaattgctc
     8221 gatgcggtga tcgaccgcaa cgccgacatt ctggagcgcg ccggcggcga catggacaac
     8281 atcagccacg acattttcga gcccgagggt gccgcgcgca ccggccactc caagcgctat
     8341 tccgatatcc tgatcgcgat cggccgcaag ggcgatctca cctccaaggt gcgcgagagc
     8401 ctggtttcga tcgggcgggt ggtgacgttc gtcgccgccg gcaccgaagg cgtcaaatgg
     8461 tccaaggaca tgcgcgagca gctcaaaatc atgcagcgcg acgtgacctc gctgaccgac
     8521 cacgcctcct atctctccaa caagatcacc ttcgtgctgg acgcgatgct cggtgtcgtc
     8581 aatctcgagc agaacaacat catcaagctg ttctcggtga tggcggtggt gttgatgccg
     8641 ccgaccctga tcgcctcgat ctacggcatg aatttcaagt cgatgccgga gctggaatgg
     8701 acccacggct atccctacgc gctggtggcg atgctgctcg ccgcggtcgg gccttacctg
     8761 tttttcacat ggaagaagtg gctgtaggtt cagtgggtga cacgagtgca cgaccgtcat
     8821 acgcgggctt gacccgcgta tccatcaccc tgtcaggagt cttgtgaaga agatggattg
     8881 ccggatcaag tccggcaacg acggcaacgc cagtcccaaa ggcggagcag agttaagaaa
     8941 gtgcgtcctg gattgaatgc agtacatcgc gccgtccacc tcaatttctc ccgtaaaatt
     9001 tgagtgttgg actgaacgtt tacgcgacag ctttctgcga taacgttgtc tcaaggcgcg
     9061 aggacagcaa tggttgagaa agtcataact gcatcggaga acgtggttga tttcgcgcgc
     9121 tatcaagtgg atcgcaaggc cggcaaggcg ctcgcgatct cggcccgggc ctgccggcat
     9181 tgcggcgcag tgctgtcgga gggcgagagc gaagacgagt gctccagcgc cttcaacatc
     9241 gacaacgccc gccttcgctg gcaggccgcg caaatcctgc gcggattgat ttgagcgggt
     9301 cgcaaacacc gccgtcattc cgggatgcgc cacccgacct cgggtttacc cgagttcggg
     9361 caatatcgtt gtccaagtcg gcaacagccg acttggatgc gcaggcccgg aatccatact
     9421 ccccgtcgtg gttatggatt ccgggctcgc gctgagcctg tcatcgggcg gcgcagagcg
     9481 ccgacccgtt ggcgcgcccc ggaatgacga gaagctaaac gtgctgcccg ccgttgatgt
     9541 ggatctcggc gccgttcacg taagaactgg tctccgtgca cagcacatag atgatcttcg
     9601 ccacttcgtc cggggtgccc agccgatgca gcgggatctg ctgctcgacg atcctttcgg
     9661 tgccgggcga caagatcgag gtgtcgattt cgccgggcgc gatcgagttg acgcggacgc
     9721 cgatgcggcc gaaatccgat gccatctcgc gggtcagtga cgccagcgcc gccttcgagg
     9781 tcgcataggc cgcgcccgcg aagggatgca ccctcgagcc cgcgatcgag gtgacgttga
     9841 ccaccgagcc tttcgcggcc ttcaattcct cgatcagccc gcgcgccatc atgatcggcg
     9901 cgaagaaatt gacgcggaac acgtggctcc aggtgtcgat gtcggtatcg atggagccga
     9961 gccggccgcc gcctgcggcc ttcggggaaa tcgcggcgtt gttgaccagc gcgtgcagat
    10021 cgccgccctc cagccgctcg cggatttccg aaatcgcccg cgtggtgtcg gcgtggtcgg
    10081 caaggtcgac ctggatgtgg tcttcgggtc cggcgcccca cgggcactct tccgggaacg
    10141 ggtgtcgcga gcaggtgatg acgcgccagc cggcgctgga aaaccggatc acggtggcgt
    10201 gcccgatgcc gcggctggct ccggtcagaa gcagcgtacg ccgcggcgcg ttggttgaac
    10261 tcggcatgga agtccttcag gctctgggcg tcagggataa agccgcgtcc tggaccatgg
    10321 ctgatccggc gtagcgcggc ggaattcgat gcggtcgtgc aggcggaacg ggcggtcgtg
    10381 ccagaattcg aactgcagcg gcgcgatgcg ccagccgctc cagccaggcg gccgcggcac
    10441 ctcgccgaca aggtgccggg cggcaatttt tgcaatggcc tgctcaaagg caaagcggct
    10501 ttccagcggc tgcgactgct tgctggccca ggcgccgatc tgggcctgtt ttgggcgcgt
    10561 ggcgaaatag gcgtcggcct cggcgtccgt caccggcgac acgttgccgc ggatccgaat
    10621 ttgccggcgc agcgatttcc agtgaaacag taaagccgcc ttaggatttg tggcgagttc
    10681 gcgccccttc tggctcgcga tgtggctgta gaacacgaaa ccactggcgt catagccttt
    10741 catcagcacc attcgcacgt ccggcagccc atcggcgtcg acggtggcca gcgacatggc
    10801 gttgggatcg ttgggttcgg ccttcacggc ctcggcgaac cactcgccga acagcgcaaa
    10861 cggttcctcc gccgcggtga aatcaccgga tgttaacggt gtggggtgtt tgatggaggt
    10921 cgtgtcggtc atatcaggag tccgagttgc gttcgcccgc ggcccagagc gcgttgcagc
    10981 acttatatag gctcgcccta tataaggcat gggaacgcgt tggcctatcg gcgatcgccc
    11041 cggcgggcgc cgtgatgata ctgattctaa tcggcgccgg ctcaggcggc tgcagcctgt
    11101 cccgcaccga cggcgccttc gccaggatgg aagaccacgg ggtcacgggt tcggtgggcc
    11161 tcgcgcaggc aggcggcctg atgccaaccg agagcgatct tgcctttgcc cgcaatgccg
    11221 cctccgacgt gctgaccaag gggaccaagg attccagcca gccctgggag aatccggaga
    11281 ccggcgcgcg gggctcggtg acgccgctgg cggaggccta tgcctcggat gggcgcacct
    11341 gtcgggactt tctggcaagc tatgtcaacg gccggtcgga aagctggctg cagggcgccg
    11401 cctgccaatc cgaacagggc cggtgggaaa ttcacacgtt aaaaccgtgg cggcggggtt
    11461 gaacgatcaa tgcccgttgc aaaaatgcca cgaagccccc aaatgaaaat tgggacggcc
    11521 cggtagcccg gagtctaaga tctgattccc ttaaaggaga cgtgacggat gcgtgacccc
    11581 tatgaggtct tgggggtgcc gcggggcgcc agcgctgcgg cgat
//
LOCUS       DATNOI010000533        11765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_761798,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000533 DATNOI010000000
VERSION     DATNOI010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11765
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761798"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="VN968_21570"
     CDS             <1..1443
                     /locus_tag="VN968_21570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31859.1"
                     /translation="LDRGRLGLGGVVLPMRSSSTPLTNSQVFALPSMAPVRKALDDEF
                     DRYVARHKASLPNETIGVGASFDFQLFDRKLLYSRDSRFVLSGIVNRMDRAYLSEANC
                     GEIRLIYRLTRTSAPETSQNATSPRLPMTLNVVLKARTDNATDGSGVKITCAEIARRW
                     LAFGESTLTGVELAQKLTSKDGPLGLIRPENIDRIETNLQIAHAPKSAVRDFRTDYLL
                     KVFDYKAQSGTFEEAPLENQIDRERIFADEALKRDFKAWLLDSGHFGELDRGAILVPK
                     KFLARSAIAATPVGFAASDLQPAFGLLQADDAKADTVFRESDVVEALKRAADAGVTLQ
                     NIRSPAGFERRLNDMSCSGCHQTRGIGGFHFPGVDWMAAKPDNSTVVPASPHFFGDQI
                     RRRDILTSMRDGSRPDYSRGFSGRPQLRGSTELGGTEYEDGWGALCYQQKPDTKDNDL
                     SFRSWSCAEGLACQVVDKASRTGMCFVRDR"
     gene            2128..3366
                     /locus_tag="VN968_21575"
     CDS             2128..3366
                     /locus_tag="VN968_21575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018856979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HXL31860.1"
                     /translation="MDDIIIVGAGIGGLTLGLALHAAGIPCRIFESAAEIRPIGVGIN
                     LLPHATKELAALGLEADLARVAIPTQDATFFNRFGQLIYREPLGRAAGYEHPQFSIHR
                     GDLQMVLLNAFRARAGHDRLLTNHHCIGVEQNESAVSVDFSDGPGGSHRSTVRGRAAI
                     ACDGINSAIRKQFFPSEGEPRYSGVNMWRGVTRWKPMLSGASMVRAGWLSHGKMVIYP
                     IRQASGADGLQLINWVAEIETETYRKRDWTRPGSIEDFIGAFADWHFDWLDVPAFIRA
                     ADHVLEFPMVDQDPLPRWSFGRVTLLGDAAHPMVPRGSNGAGQAILDARALTSALLEN
                     RDSVAALAAYEKERLGATTRIVLTNRTNPPDAILREVFQRTNDRPFAAIGDVISRDEL
                     VALSEGYKRIAGYSKDALRT"
     gene            3469..4875
                     /locus_tag="VN968_21580"
     CDS             3469..4875
                     /locus_tag="VN968_21580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL31861.1"
                     /translation="MAIQVRGSPSPVDVAQFIDAQPVGGFQIRLLLTCAAVLFLDGFD
                     TQAIGFVAPALAREWDVSKAALGPVFSAGLFGLMIGALLFGPLADRIGRKKIIVFSTL
                     AFGIGTLVTAFVQDIDMLLAIRFLTGLGLGGAMPNAIAMTSEFNPRRRRATMVMIMFC
                     GFSVGAALGGFLAAALIPQFGWRSVFVVGGLAPLLLVPILVRRLPESVRFLALTGRAQ
                     ERVAELLWSISPRAVFAPATQFVVHEPELTGIPVLHLFRDGRTLVTLLLWVVFFMSLL
                     DLYFLSNWLPTVLNDLGATVSEAAMIGSMLQVGGVVGTFALGSIIDRFSFRALALVYF
                     IAVFAVGAIGQLGHSVIFVSMAIFAAGFCIVGGQIAANALAAGFYPTAVRATGVGWAL
                     GIGRVGSIVGPLVGGALLIAKWSTGSVFMAAAGAAFCAALAAFSLGRIAGMGGSGKGA
                     DQVASFEAGLRPSTTAGT"
     gene            4907..5074
                     /locus_tag="VN968_21585"
     CDS             4907..5074
                     /locus_tag="VN968_21585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31862.1"
                     /translation="MSDADKENKLRNREIADNEAERQTVGDIRVSVWAIAAAVVIGLA
                     AVGWVVLIRNS"
     gene            complement(5255..6460)
                     /locus_tag="VN968_21590"
     CDS             complement(5255..6460)
                     /locus_tag="VN968_21590"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXL31863.1"
                     /translation="MEDGIFKGLKVLDCASFIAAPAAATVLSDFGADVIKIEPPVTGD
                     PYRHLPNLPGYPRSEHNFAWMLESRNKKSLALDLAKPEGQAVLHRLAGDADVFITNFP
                     PAVRERLGLTYAKLAPLNERLIYASFTGYGEKGEEANKPGFDSNAYWARSGLMDLVRA
                     DTNTTPARSVAGMGDHPCAMAFYGAIVTALYKRERTGKGSQVRSNLMANGIWANGILA
                     QAKLCGAQFSERRSREQALNAVTNHYQCKDGRWIILSLLNEERQWPAFARCIGREDFV
                     TDPRFATKADRHARSQQLIGILDEIFATRDLAEWRRILDGNGLVFGVIGILDDIPGDQ
                     QMLENDVLVPFENDTLLTINSPIWIDGSQKTAPRRPPGIGEHSDEILRGAGYDEAAIR
                     QLRASGAVA"
     gene            6554..6817
                     /locus_tag="VN968_21595"
     CDS             6554..6817
                     /locus_tag="VN968_21595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31864.1"
                     /translation="MLVPRCNGIAWSQALLAMTPFFGREPERAKNVLDATDAVIGVHG
                     VDRSRGGTIDTTVLIRPQSFCFKKARRHDPIVRRAGCRERQTE"
     gene            6992..7558
                     /locus_tag="VN968_21600"
     CDS             6992..7558
                     /locus_tag="VN968_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31865.1"
                     /translation="MPARAGELSAELAALYATVSIFPPSAKSMTVCYGFVCRRREIFD
                     FTPADRNALSKILAAGRASAAAERAAVQKAVIWFDRRMGPVIGTNKRVAKADFRYFDD
                     KHNYDCWDTTRNTTSLLLVLQAWGLLKYHVVGDPHYRGNALVLQTPHNTAVLIERATR
                     IEWVVDMWPRGYLQPPDVMTVEKWVKLD"
     gene            complement(7816..8016)
                     /locus_tag="VN968_21605"
     CDS             complement(7816..8016)
                     /locus_tag="VN968_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008563500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXL31866.1"
                     /translation="MAKGTVKWFNATKGFGFIQPSSGGKDVFVHISAVEKAGLSSLNE
                     GQTVEYEEVANRGKTSAENLKV"
     gene            complement(8320..9480)
                     /locus_tag="VN968_21610"
     CDS             complement(8320..9480)
                     /locus_tag="VN968_21610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HXL31867.1"
                     /translation="MNIKAILAANGRDVRLELFLGIANWFIFLDHIPNNVVSWITIRN
                     YGFSGAADLFIFISGYIAAIVYARMTLERGFVVAATRIFKRVWQLYVAYVVLFAVYTV
                     TIGDVAARYAAPDIIYEFNVAGLVDHPIRTVGHGLLLQSRALNLDGLPLYIVLMAWLL
                     PVLWMMLRKPDLTMAGSLALYLAARQFEWNLPSFPDGSWYFNPFCWQLLFVFGAWLAL
                     GGASERRSIQTSRIALYFGIAYLAFALVMTMAGRFPAFGNMFPTWLFDAFNPNDKTNL
                     APYRVLHFIVMAYLVTRFVPKDWRGLEWQIFKPIIICGQQSLAVFCVGVLLSFVGHLA
                     LVTSSGSLLAQLFVSAAGIAIMTLVATYISWSKQQDAPHRDPHHLASADAHS"
     gene            9614..9736
                     /locus_tag="VN968_21615"
     CDS             9614..9736
                     /locus_tag="VN968_21615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31868.1"
                     /translation="MAVELLSKPQFPPPWRGDHGNRLMAYTELLPRVQGAPATS"
     gene            complement(9733..10056)
                     /locus_tag="VN968_21620"
     CDS             complement(9733..10056)
                     /locus_tag="VN968_21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXL31869.1"
                     /translation="MARCFFAALLLAFAAFAGLAPGSATAAPVSYTLPDETAAFKPGP
                     NLEVVQNNCTGCHSADYISTQPRGPKFKKDFWQAEVTKMIKLYGAPIDDADVGRIVDY
                     LAATY"
     gene            complement(10068..11267)
                     /locus_tag="VN968_21625"
     CDS             complement(10068..11267)
                     /locus_tag="VN968_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXL31870.1"
                     /translation="MIDRREIMKRAGLAALATSFGSATAFALDTVTLPFDNGERPLVK
                     YPQKRPMIGLTSRPPQLETPFSIFNDGAITPNNAFFVRYHLADVPLNIDPDKFTLEIK
                     GKVDRPKKLSLKEIKKLPAIELVAVNQCSGNSRGFSNPRVAGGQLANGAMGNARWRGV
                     TLKAVLDKAGVQQGARQVTFGGMDGPVSDKTPDFVKALDIDHAGDGEVMLAYGMNGED
                     LPVLNGFPLRLIVPGYYGTYWVKHLNEITVIDSVFDGFWMKTAYRIPDTPCGCVEPGT
                     APKATIPINRFTVRSFITNVTDGAKLKPGATTLKGIAFDGGKGIKEVAVSLDGGNSWT
                     PAKLGKDLGKYSFREWKLPVKLSAGNHELKVRATNNAGDTQPSEPLWNPAGYLRNVIE
                     TVRVTAA"
BASE COUNT         2165 a         3557 c         3655 g         2388 t
ORIGIN      
        1 ctcgatcgcg gaaggcttgg gctgggcggc gttgtcctgc ccatgagatc gtccagcacc
       61 ccgctcacca acagccaggt gttcgcgctg ccctcgatgg ctcccgtccg caaggcgctc
      121 gacgacgagt tcgatcgtta cgtcgccaga cacaaggcca gtcttccgaa cgaaaccatc
      181 ggcgttggag cgtctttcga ttttcagctg ttcgatcgca agctgcttta ttctcgtgat
      241 tcacgcttcg tgctgtcggg catcgtcaac cggatggatc gcgcttatct gtccgaagcc
      301 aactgcggtg aaatcaggct gatctaccgt ctgacacgga cgagtgcgcc cgagacgagc
      361 cagaatgcga cctcgccgcg gctgccgatg acgctcaacg tcgtgctgaa ggccaggacc
      421 gataacgcga ccgacggcag cggcgtgaag atcacgtgcg ccgagatcgc gcgacgctgg
      481 ctggctttcg gcgaatcgac gctgaccggc gtggagcttg cgcagaaact gacatcgaag
      541 gatggcccgc tgggtctgat ccggcctgaa aacatcgacc gcattgaaac caatcttcag
      601 atcgcgcatg cgccgaaatc ggcggtgcgc gatttccgca ccgactatct cctcaaggtg
      661 ttcgattaca aggcgcagtc cgggacgttt gaagaagcgc cgctcgaaaa ccagatcgat
      721 cgcgagcgaa ttttcgcgga tgaggcgctc aagcgcgact tcaaggcgtg gctgcttgat
      781 agcgggcatt ttggcgagtt ggatcgcggc gcaattctgg tcccgaagaa gttcctcgcc
      841 cgcagcgcga ttgccgcgac cccggttgga tttgccgcct ctgacctgca gccggccttt
      901 ggcctgttgc aggccgatga cgcaaaggct gacaccgtgt tcagggaaag tgacgtggtc
      961 gaggctttga agagggcagc cgacgccggc gttacgttgc aaaacatccg gtctcccgcg
     1021 ggcttcgagc gccggctcaa cgacatgagc tgttcaggat gccatcagac ccgcggcatc
     1081 ggcggctttc acttccccgg cgtcgactgg atggcggcca agcccgacaa ttcgaccgtc
     1141 gtgccggcct caccgcattt cttcggcgac cagatccgcc gccgcgatat tctgacctcg
     1201 atgcgggacg gtagtcgccc ggattattcg cggggattct ccggccgccc gcaattgcgc
     1261 ggcagcaccg aactcggcgg caccgaatac gaggacggct ggggcgcgct gtgctaccag
     1321 cagaagcccg acaccaagga caacgatttg agctttcggt catggagctg tgccgagggg
     1381 ctcgcgtgcc aggtcgtcga caaggcctcc cgaacgggga tgtgctttgt cagagatcgc
     1441 tagatagagc atgatccgga aaaagcctgc cccgcacttg atgcggggtg ggtaccggtt
     1501 ttcccccggg acaaacgcga agcgtttgcc cggagatcat gctcaaacaa aaagatagag
     1561 cgggatgacg attcgaagaa aggtcatccc gctctagtgc cgttgccttg tcgaggtctg
     1621 gaactgtgtt cacgtcgata tctcctcggg ctcgcgcatc ggaaagcgcg cgcggctcgg
     1681 caacgcgaga ggcgaccctt cgctgtggcg agccgatgca acttcaattg tgggtaagga
     1741 ttttttacct gcaatcgcgc tagaggagcc cgttgtcggg cagggccatg ggcgtcttgc
     1801 aaattgcgcg atgatagaac agtgccgctg atttgcccga cgtgtcaaat gttttcgcag
     1861 ggatagctca aggcatccat gccagcgacc gccatgctac tttgcatggg gttgttttcg
     1921 atattttagt tggaagccag agcctggtct taccagaact cgtcatgaac cggcgatcgg
     1981 agccacaaaa tccacacgcc tcgtcggtct gaggcgaagc ctcgctagga aatagctctt
     2041 ctgatggcgc ggcagccaga ttgacagtgg tctcactaat cgtgtttcat tccggcaatc
     2101 gcctgacagc gcgcgccggg agcagacatg gacgacatca ttattgtcgg ggccgggatc
     2161 gggggattga cgctggggct cgcgctgcac gccgccggaa ttccctgccg gatttttgaa
     2221 tcggcggccg aaatcaggcc gatcggggtc ggcatcaatc tgcttccgca tgccaccaaa
     2281 gagcttgccg cgctcgggct tgaagctgat ctggcgcggg tcgcgatccc aacccaggac
     2341 gcgactttct tcaatcgctt cggtcaattg atttatcggg agccgctggg acgggcagcg
     2401 ggctacgagc atccgcaatt ctccatccat cgcggcgatc tgcagatggt cttgctcaat
     2461 gcctttcgtg cgcgcgcggg acacgaccgg ctgctgacca accatcactg catcggagtt
     2521 gaacagaatg agtccgctgt cagcgtcgat ttttccgacg ggccgggagg cagccaccgc
     2581 agcaccgtgc gcggccgcgc cgcgatcgcc tgcgacggca tcaactccgc gattcgcaag
     2641 cagtttttcc ccagcgaagg tgaaccgcgc tattccggcg tcaacatgtg gcgcggcgta
     2701 acgcgatgga aaccgatgct gtccggcgcc agcatggtgc gggcgggctg gctgtcgcac
     2761 ggcaagatgg tgatctatcc gatccgtcag gcctccggcg cggacgggct gcagctcatc
     2821 aactgggtgg ccgaaatcga gacagagacc tatcgcaagc gcgactggac ccggcccggt
     2881 tcgatcgagg atttcatcgg cgcgttcgcc gactggcatt tcgactggct cgatgtgccg
     2941 gcgtttatcc gcgccgccga tcatgtgctg gaatttccga tggtcgacca ggacccgctg
     3001 ccgcgctgga gtttcggccg ggtgacgctg ctcggcgacg ccgcccaccc gatggtgccg
     3061 cgcggctcca acggcgccgg ccaggcgatt ctggacgcgc gggcgctcac ctcggcgctg
     3121 ctggaaaacc gtgattccgt tgcagccctc gcggcctatg agaaagagcg gctcggagcc
     3181 accacccgca tcgtcctgac caaccgcacc aatccgcccg acgcgatcct gcgcgaggtg
     3241 ttccagcgca ccaacgaccg gcctttcgcc gccatcggcg atgtcatcag ccgcgacgaa
     3301 ctggtcgcct tgtcggaagg ctacaaacgg atcgccggct attcaaagga tgcattgcgc
     3361 acctgacgga gcagggtatc cttcctcttg ccgaattcgc gccagactat cgcgtcagga
     3421 atacgggtcg agaagaaccg cggcccgacg tttcagagag ggacaatgat ggcgattcag
     3481 gttcgaggca gcccgtcgcc ggtcgatgtc gcccagttca tcgatgcgca gccggtcggc
     3541 ggatttcaga tccgattgtt gctgacctgc gcggccgtgc tgttcctcga tggcttcgac
     3601 actcaggcga tcggtttcgt cgcgccggcg ctcgcccggg aatgggacgt gagcaaggcg
     3661 gcgctcggtc cggtgttcag cgccggcctg ttcgggctga tgatcggcgc gctgctgttc
     3721 gggccgcttg ccgaccgcat cgggcgcaag aagatcatcg tgttctcgac cctggcgttc
     3781 ggcatcggca cgctggtcac ggcgttcgtc caggatatcg atatgctgct ggcgatccgg
     3841 tttctgaccg ggcttggtct cggtggcgcg atgccgaatg cgattgcgat gacctccgag
     3901 ttcaatccgc gtcgccgccg cgccaccatg gtgatgatca tgttttgcgg gttctcggtg
     3961 ggggcagccc tcggcggctt tctcgcggcg gcgttgatcc cgcaattcgg ctggcgctcg
     4021 gtgttcgtcg tgggcgggct cgccccgctg ctgctggttc cgatcctggt gcggcggctg
     4081 ccggaatcgg tgcggttcct cgcgcttacc ggccgcgcgc aggaacgcgt cgccgaactg
     4141 ctgtggtcca tcagcccgcg cgccgtgttt gccccggcaa cgcaattcgt cgtccacgag
     4201 cctgaactca ccgggattcc ggtgctgcat ctgttcaggg atggccgaac cctggtcacg
     4261 ctgttgctgt gggtggtgtt cttcatgagt ctgctcgacc tctatttcct gtcgaactgg
     4321 ctgcccacgg ttctcaatga cctcggcgcc acggtctcgg aagcggcgat gattggatcg
     4381 atgctgcagg tcggcggcgt ggtcggaacc ttcgcgctcg gcagcatcat cgaccgcttc
     4441 tcgttccgcg cgctggcgct ggtgtatttc attgccgtgt tcgcggtcgg cgccatcggc
     4501 cagctcggcc attccgtcat tttcgtcagc atggcgatct tcgccgccgg cttctgcatt
     4561 gtcggcggcc agatcgccgc caacgcgctg gccgcggggt tctatccgac cgcggtgcgg
     4621 gcgaccgggg tcggctgggc gctcgggatc ggacgggtcg gctcgatcgt ggggccgctg
     4681 gtcggcggcg ccttgctgat cgcaaaatgg agcacgggat cggtcttcat ggcggccgcg
     4741 ggcgcagcct tctgcgcggc gctggcggcg ttttccctgg gccggattgc cggcatgggt
     4801 ggcagcggca agggtgccga tcaggtggct tccttcgagg cgggcctgcg tccgtcgacg
     4861 actgccggaa cctaggctag actgcgccgg caacggagga ccggcgatga gcgacgcgga
     4921 caaggagaac aagttgcgca atcgcgagat cgccgacaac gaagccgaac ggcagaccgt
     4981 cggcgacatc cgcgtcagcg tatgggcgat cgcggctgcg gtcgtaatcg ggcttgctgc
     5041 cgtcggctgg gtggtgttga tccgcaacag ttagcgcgga tttgttttcg catggattta
     5101 attttcgtca ttccgggatg cgccgttcgc gcaggcccgg aatccatact ccctgtcgtg
     5161 gttatggatt ccgggctcgc ttgtttcgca atcgccccgg aatgacggag cccaaattgc
     5221 accttcatca ctccaaaata gaagctctaa ctgttcacgc caccgcgcca gacgcccgca
     5281 gctgccggat cgcggcctcg tcatagccgg caccgcgaag aatctcatcg ctgtgctcgc
     5341 cgattccggg tggacgccgc ggcgcggtct tttggctgcc gtcgatccag atcgggctgt
     5401 tgatggtgag cagggtgtcg ttctcgaacg gcaccagcac gtcgttctcg agcatctgct
     5461 ggtcgcctgg aatatcgtcg aggatgccga tcacgccgaa caccaggccg ttgccgtcga
     5521 ggatcctgcg ccactcggcg aggtcccttg tggcaaaaat ctcgtcgagg atcccgatca
     5581 gttgctgcga tcgcgcatga cggtcggcct tggtagcgaa tcttggatca gtgacgaaat
     5641 cctcccgacc gatgcagcgg gcgaatgccg gccattgccg ttcctcgttg agcagcgaca
     5701 ggatgatcca gcgcccgtcc ttgcattggt agtggttggt caccgcattc agcgcctgct
     5761 cgcgggatcg ccgctcgctg aactgcgcgc cgcataattt ggcctgcgcc agaatgccgt
     5821 tggcccagat gccgttggcc atcaggttcg acctgacctg cgagcccttg ccggtgcgct
     5881 cgcgcttgta gagcgcggta acgatcgcgc cgtagaacgc catggcgcag gggtgatcgc
     5941 ccatgccggc gaccgagcgc gccggcgtgg tgttggtatc cgcgcgcacc agatccatca
     6001 ggcccgagcg cgcccaatag gcgttgctgt cgaaaccggg cttgttggct tcttcgcctt
     6061 tttcgccata gccggtgaac gatgcataga tcaggcgctc attgagcggg gcaagcttgg
     6121 cgtaggtcag cccgagtcgc tcgcgtactg ccgggggaaa attggtgatg aagacgtcgg
     6181 cgtcgccggc gaggcggtgc agcaccgctt ggccttccgg cttcgcaaga tcgagcgcga
     6241 ggctcttctt gttgcgggat tccagcatcc acgcgaaatt gtgttcgctg cggggatagc
     6301 ccggcaggtt cggcaggtgg cgatagggat cgcctgttac cggcggttcg atcttgatca
     6361 cgtcggcgcc gaaatccgac agcacggttg cagccgcggg cgctgcgatg aaactcgcgc
     6421 agtcgagaac cttgaggcct ttgaaaatgc cgtcttccat cgtgaggtcg ctcccgtgag
     6481 tttttgttgg tcttgttccg caacatggaa tcgcagttcg ctgcgatccg aagattcctg
     6541 ccgcatttga ccgatgttgg tgccgcgatg caacgggatt gcttggtcgc aagcgctcct
     6601 tgcaatgaca cccttctttg gtcgagagcc ggaacgcgcc aagaacgtcc tcgacgctac
     6661 cgatgccgtc attggtgtgc acggcgtcga tcgatcgcgt ggcggcacga tcgatacgac
     6721 cgtcctgatc cggccgcaat ctttctgttt caagaaggcg cgccgacatg acccgatcgt
     6781 ccggcgcgcg gggtgccgtg agcggcagac agagtgataa ttttcgccgg gttcttggcg
     6841 tgaaagcgtg cgaccgatgc ggctgacgga caagcggcaa cgatctggca gatccggcag
     6901 agccttgttc ggcaaagcct tgttctgcaa acccttggcg tcttgcggtt tggccacgtg
     6961 ccttgtcgga tggacactga tcaccgcgcc gatgccggcg cgcgccggcg aactcagcgc
     7021 tgagctcgcc gcactctacg cgacggtttc gatttttccg ccttcggcga aatccatgac
     7081 ggtgtgctat ggctttgtct gcagacgccg cgagattttc gatttcacgc cggccgaccg
     7141 caacgcgctg tcgaaaatcc tcgcggcggg ccgcgcttcg gccgcggcgg aacgcgcggc
     7201 ggtacagaag gccgtgatct ggttcgatcg ccgcatgggg ccggtgatcg gaaccaacaa
     7261 gcgcgtcgcc aaggcggatt tccgctactt cgacgacaag cacaattacg attgctggga
     7321 caccacccgc aacaccacca gcctgctatt ggtgcttcag gcgtggggtc tgttgaaata
     7381 tcatgtggtg ggcgacccgc attaccgcgg caatgcactg gtattgcaga cgccgcacaa
     7441 caccgccgtg ctgatcgaac gcgccacccg aatcgaatgg gtcgtcgaca tgtggccgcg
     7501 cggctacctg cagccgccgg atgtcatgac ggttgagaaa tgggtcaagc tggattagag
     7561 cgttttcgag cgaaccatgc cctcggactc gatccggggg tggagaaaga aagcgcgtcg
     7621 aatcaaaaat ccagcgcccc gtttcgattc catcgaaacg gacaaggctc gagcaatcga
     7681 acaggcgcat tctgcgcccg attcgaatgc gcaagagtgg tgcccactcc tgccgagtga
     7741 attctgtttt ttggtcttct tggaagctcc actcccggat cgcgtccgag agcccgctgt
     7801 ctggccgctc tggcgttaaa ccttgaggtt ctcagccgag gttttgccgc ggttggcaac
     7861 ctcttcgtat tccaccgtct gtccttcgtt gagcgaggaa aggccagctt tctcgaccgc
     7921 cgaaatatga acgaaaacat ccttgccgcc gctagagggt tggataaatc cgaagccctt
     7981 ggtcgcgttg aaccacttca cagtgccttt agccatcacg tcgtctccgc gggatcaaaa
     8041 acgatagcga actgccggtc cccgcacacc gtcatgcccg acgcccgcac gatacgcgac
     8101 ataacgcaag cttgatagct taaacgacat tcggatttcg gcgaaaaacg atcattatcg
     8161 cggtgcattc gcgctttttg gctgtttcgc cgtcatttga cgccgcgagc gacggaaaac
     8221 gcggccggat tccggtttta ttgaattgga acctgaggcc gcccgtcccg ccggtggcgc
     8281 gcgtcgagat aaatctcgat cgagtttcag cccgtgaagt catgaatgag catcggcgga
     8341 tgccagatga tgtgggtcgc gatgtggggc gtcctgttgc ttggaccatg agatgtaggt
     8401 ggcgaccagc gtcatgatcg ctatcccggc cgcgctcaca aaaagctgcg ccagcaggga
     8461 acccgaactt gtcaccagtg cgagatggcc gacaaacgac agcaagacgc cgacgcaaaa
     8521 tacagcaagc gactgttgcc cgcatatgat gatcggcttg aagatttgcc attccagccc
     8581 acgccagtcc ttcggtacaa atcgcgtcac gaggtatgcc atgacgatga aatgcaagac
     8641 gcgatacggg gcgagattgg tcttgtcgtt agggttgaag gcgtcgaaca gccaggtcgg
     8701 gaacatgttt ccgaacgccg ggaaacgtcc tgccatcgtc atcaccagcg caaaagccag
     8761 gtaagcgatc ccgaaataaa gcgcgatccg cgacgtctga attgagcggc gctcgcttgc
     8821 cccgccgagc gcgagccagg cgccaaacac aaaaagcagc tgccagcaaa acgggttgaa
     8881 ataccagctg ccatccggaa acgacggcag attccattcg aactgacgcg cggcaagata
     8941 gagcgcaagc gagccggcca tcgtcaggtc cggcttgcgc agcatcatcc acagcaccgg
     9001 cagcaaccat gccatcaaca cgatatagag cggcagtccg tccaggttca gcgccctgga
     9061 ttgcaacaag agcccgtggc ccacggtccg aatcggatgg tcgaccagcc cggcgacgtt
     9121 gaattcatag atgatgtcgg gagcggcgta tcgtgccgca acatctccga tcgtcacggt
     9181 gtagaccgcg aacaggacga cataggcgac gtagagctgc cacacccgtt tgaagatccg
     9241 ggttgccgct accacgaagc cgcgttccag cgtcattctt gcgtagacga tcgccgcgat
     9301 atagccggaa atgaagatga agagatccgc agccccgctg aaaccgtagt ttcggatggt
     9361 gatccagctc accacattgt tggggatgtg atccagaaag atgaaccagt tcgcgatgcc
     9421 caggaacagc tcgagcctga catcgcgccc gttggccgcg agaatagctt tgatgttcat
     9481 ggagccgttt cgcaatgccg gttctaaaaa cgtgtcgctg gaatcaaggc cgtggtgggc
     9541 gggaatcgta gtgcagacgc gatggcgacc ccaatagact ttacgtgaac gccccgatgc
     9601 gtgaacgggc gcgatggcgg tggaactgct gtcgaagccg caattcccgc cgccatggcg
     9661 cggcgatcac ggcaaccggc tgatggcgta caccgaattg ttacctcgcg tgcaaggcgc
     9721 tccggcgacg tcctaatagg tcgcggcgag ataatcgacg attcggccga cgtcggcgtc
     9781 gtcgatcggc gcgccataga gcttgatcat cttcgtcact tcggcctgcc aaaaatcctt
     9841 cttgaatttc ggcccgcgcg gttgcgtgct gatgtagtcc gccgagtggc agccggtgca
     9901 attgttctgc accacctcga gattgggtcc aggcttgaag gctgcggttt cgtccggcag
     9961 tgtgtaggag accggcgccg cggtcgccga accgggagcc aggcccgcga aggcggcaaa
    10021 tgcgagtaga agcgctgcga aaaaacaacg ggccatgacc gatctcctca cgctgcggta
    10081 acgcgaacgg tttcgatgac attgcgcaaa tagcccgccg gattccacag cggctcggag
    10141 ggctgggtgt cgccggcatt gttggtcgcg cgaaccttca gctcatggtt gcccgccgaa
    10201 agcttcaccg gcagcttcca ttcgcggaac gaatattttc cgagatcttt tccgagtttt
    10261 gccggtgtcc agctgtttcc gccgtcgagc gagaccgcga cctccttgat gcccttgccg
    10321 ccgtcgaagg caattccttt cagcgtggtt gcgcccggct tcagtttggc gccatcggtc
    10381 acattggtga tgaaggagcg gacggtgaag cggttgatcg ggatcgtcgc cttcggggcg
    10441 gtgccgggct cgacgcatcc gcagggcgtg tccggaatcc gataggcggt cttcatccag
    10501 aagccgtcga agacgctatc gatcaccgtg atttcgttga ggtgctttac ccaataggtg
    10561 ccgtaatagc cgggcacgat caggcgaagc ggaaagccgt tgagtaccgg cagatcctcg
    10621 ccgttcatgc cataggccag catcacctcg ccatccccag catggtcgat atcgagcgcc
    10681 ttgacgaagt ccggcgtctt gtcgctgacc ggtccgtcca tcccgccgaa ggtgacttgt
    10741 ctcgcgccct gctgcacgcc ggccttgtcg agcacggctt tcagcgtcac cccgcgccag
    10801 cgcgcattgc ccatcgcgcc attggccaac tgaccgccgg cgacgcgcgg attggaaaag
    10861 ccgcggctgt tgcccgagca ctgattgacg gcaacgagct cgatcgcggg aagctttttg
    10921 atttccttca gcgacagctt tttcggacga tcgaccttgc ccttgatctc cagcgtgaac
    10981 ttgtccgggt cgatattgag cggcacatcc gccagatgat agcggacgaa gaaggcattg
    11041 ttcggcgtga tcgcgccgtc gttgaagatg gaaaacggcg tctcgagttg cggcggccgg
    11101 ctggtcaggc cgatcatcgg ccgcttctgc ggatacttta ccagcggccg ttcgccgtta
    11161 tcgaacggca gcgtcacggt gtcgagcgcg aacgccgtgg ccgaaccgaa gcttgtcgcg
    11221 agtgccgcca agcctgcgcg cttcattatt tcacgtctgt cgatcattcg cttcctccga
    11281 agcttttttg ctgaggccgt cctcatcagg acgccaccga cttgcgttta ttccgttcac
    11341 aagaccggca tggactctcg aatcaaatcg agagcatgct ctcgtaatat gcgcttgtgc
    11401 gattgtcgca ctggcaaggc ccaagtcaat ctacaagtca attcatgctt tcgaagcgcg
    11461 cacaggccgc tgcgccgcaa taatcgcagt cgagctgaca gataatctgg cccaaccgcg
    11521 tcagtcgttc cccggatgct gcgcagcgcc acccgcgcgt ttacgcgcgt cttcgacgcg
    11581 ccatcagcgc gtttacgcgc gtcttcgacg cgctatggac ttgcggcgta ggtgatttgc
    11641 ccgtcgggcg gcttgttgac aggggtgtcg agtccctttt gcaaaaatat ttctgttttc
    11701 atacccccca aatcacttct agaacttttc gcatcccgtc ccacacagag gggcgtttcg
    11761 cgatc
//
LOCUS       DATNOI010000534        18444 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1231900,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000534 DATNOI010000000
VERSION     DATNOI010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18444
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231900"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /gene="phnK"
                     /locus_tag="VN968_21630"
     CDS             <1..132
                     /gene="phnK"
                     /locus_tag="VN968_21630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HXL31871.1"
                     /translation="RLLSHRVTVMKAGRVIETGLTDQVLDDPREPYTQLLVSSILPA"
     gene            144..842
                     /gene="phnL"
                     /locus_tag="VN968_21635"
     CDS             144..842
                     /gene="phnL"
                     /locus_tag="VN968_21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HXL31872.1"
                     /translation="MTAMVDISDAEKTFTMHLQGGVELPVVRGVSFQVRAGECVVLSG
                     PSGAGKSSILKMIFGNYRCDAGRIGICHEGVQIDLAKAEPRQILSVRRATIGYVSQFL
                     RAVPRVAAIDVVAEPLIANGAARGHAREQAGSLLRALNIPQRLWALPPSTFSGGEQQR
                     VNIARGFISDLPILLLDEPTASLDAANRAVVVDLINEKKRAGVAMVAIVHDDEIRHLI
                     ADRVVDVTSFAAAA"
     gene            858..2015
                     /locus_tag="VN968_21640"
     CDS             858..2015
                     /locus_tag="VN968_21640"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HXL31873.1"
                     /translation="MTSTRDETVLGNARIVLADRVIERGWIAFADGRVAEVGEGDGPV
                     GSQDAAGDLIMPGLIELHTDHLEAHYVPRPKVFWDPIAAVVSYDGQLATSGITTVLDS
                     LRVWREDGAEEVDGRAGILAEAIASARDADLLRADHFLHLRCEIPMPSVVEEASELVG
                     RPDVRLMSLMDHTPGQRQFRDEDKLRTYYRGKGAGMTDAELDTLFERRFTYQKAHAAT
                     NMREIVALAQQYEIPLASHDDTTDENVTDAIRDRVSVAEFPTTIDAARGLHRAGIGIL
                     MGAPNVVRGGSHSGNIAAVDLAREGLLDILSSDYIPSSLLMAALQLPQRVPAIDLASA
                     VRTVTKTPAEAVGLTDRGAIAVGKRADLIRVHIARDIPVVRSVWREGHRVA"
     gene            2012..2596
                     /gene="phnN"
                     /locus_tag="VN968_21645"
     CDS             2012..2596
                     /gene="phnN"
                     /locus_tag="VN968_21645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism
                     protein/1,5-bisphosphokinase (PRPP-forming) PhnN"
                     /protein_id="HXL31874.1"
                     /translation="MTEASAKSDHPPAAIGPGRLILVVGPSGAGKDTLIGWAAAACAN
                     DGNIVFPRRVVTREASASEDNEQVSLDAFLHARSRDEFAVQWEAHGHCYALPRAINDD
                     IRGGRTVVANVSRTVVDAVRRAYAAVTVVSITAPPDILAERLAARARSSDGQLADRLR
                     RAVDDAAAVPDVTIINVGSVEDRAREFVQVIRKA"
     gene            2644..3255
                     /locus_tag="VN968_21650"
     CDS             2644..3255
                     /locus_tag="VN968_21650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HXL31875.1"
                     /translation="MTIIATIGELEAIYGQPNEASTVKVADRITPQYRVLIDKSPFAA
                     LATCGPEGLDCSPRGDLPGFVRAHDETTLMMPDRRGNNRVDSLRNIVRDPRIALLFLI
                     PGSGSTLRVNGRARVSADADLLASFKVDGKAPRTVIVMTVDEIYFQCARAIVRSDLWN
                     PDRRVDPKSLPTPGQILAEMSENRVGGEDYDRAWPERARQTMW"
     gene            complement(4004..6280)
                     /locus_tag="VN968_21655"
     CDS             complement(4004..6280)
                     /locus_tag="VN968_21655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31876.1"
                     /translation="MFASITRNNLQCIVPPSATRLRGFFTDLLHLGRPQERAMKRLKD
                     FLGASIPLALGAVFLGAASQSASAACLLNSPGGQVKHVVHIVFDNVHLRRDNPNVPSD
                     LEQMPNLLNFLQGRGVISGNHYTPLISHTADDILTGLTGVYGDRHGSPVSNSFGIFKK
                     DNSAAVFQTSFVYWTTTTAQSHPEAGENLPVLLNENGKNAPAPWVPYTRAGCDVGAVS
                     LNGLAIENKTDIANIFGSSVAAGAATQDYEGIAIHCAPGSGLCNNPSFARPDLLPDEP
                     GGYNGFSALYGNINVQPVISPSGPVTDLDNVVITDPFSNKPGFPTDFSPSATASLGYA
                     AKMLEAGVPVVYTYVADAHDPHRQDIPNSGPTPNSSHAYGPGEAGYVAALKAYDTAFG
                     KFFARLKADGIDETNTLFVAVPDENDHFVGGAPTPANCDGITTPCTYTKVGEIEASLD
                     KLLLAQRMNSTPFSVHSDDAPTMYINTNPGPTDTLTRTMEHDVDALVATSPITGNNDK
                     LSKLLADRAEMKLLHMVTKSPARTPTFTIFGNDDYFFETGNNNGCTTPTDVVCEAPGF
                     AWNHGDFQKQITQTWVAMVGPGVKRLGRDDDVFTDHADVRPTLMALLRLTDDYVHEGR
                     VVAEFMQDNALPPGIRKSRENFVELAQVFKQLNAPKGELGRASLVWSNRSVTSTDKVY
                     ARYLKRIDDITGDRDQLASQIKTVLNNAAFHNQAVEEGAEDGLGHRAKALIDLVKDLA
                     EKGEHDHRDGDHDHRAEN"
     gene            complement(6448..6909)
                     /locus_tag="VN968_21660"
     CDS             complement(6448..6909)
                     /locus_tag="VN968_21660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL31877.1"
                     /translation="MLVGAGTTRAAFADGLSRATAAYSRGDYVRAANELSPLALRGNA
                     RAQTLLGFMYENGFGVPQAYDAATDLYQKAAVQGDPSAQSRLGLSYDKGHGVPQSVVL
                     AYKWLDLAAAHAPRRERDYYLRLRNAVASKMSSDQIVEGQRLALFWAPGRW"
     gene            complement(7013..9271)
                     /gene="gspD"
                     /locus_tag="VN968_21665"
     CDS             complement(7013..9271)
                     /gene="gspD"
                     /locus_tag="VN968_21665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HXL31878.1"
                     /translation="MLLSAGLLASCNAVTMGSGSGADIDVLDKVRSLDTLPRYPQQAN
                     AAEANVGQRSRPVMFEGTEVSDVAETRPQPVATGNGFELNFENTPVATVAKVVLGDIL
                     QTGYTIDPRVQGTVSLVSVRPVAKSDIVFVLENALRLSGIVLVRDTAGYRLTPLGDAV
                     GAGRIDAAASSPEPGYGVSVVPLQYVSAQTLLKLMDSFATKAGMVRADTTRNLLLIQG
                     SGAERRTAVDTALSFDLDWMRGQSIGIFPIVNSGPEPVIAELEKIVDSGDNGLSQNLI
                     KFQAVSRLNAILVVSKKPGLLHTAATWIKRLDHADTARTSVHVYRVKYGEARQIARVL
                     TEMFIGGSSSGLLDSPDSQIAPGSGTYATASADRLSLNSNSPSSTSSSGFGSRMQSGT
                     NGNSGFGAQTTAAPAGANANTSDAGSLDSRGAGSGGQPMLKGVRITADTVNNSLLIYA
                     DQENFRIIEATLQQVDQPQLQVAIDATIAEITLTDELSYGVQSYLTSHNLGLRPDQGS
                     ILNTQSTTAPVAAAAGTVANAFINRAFPGFNFLIGSEAQPSAILDALHTVTAVKVLSN
                     PSLVVVNNQAATLQVGDSVPVSTGSATVLTTNNTVVNTIDYRNTGIILRVSPRINVNG
                     NVRLDVEQEISNVSAATAASLTPTVSERKVKSSISVATGQTVLLAGLISEQHNNQRNG
                     IPLLDQIQGLGDAFSHQDNKATRTELIIFIRPQIIRDSMDAHFVAEELRSKLRGSINA
                     SVANDTRVQGVR"
     gene            9562..9690
                     /locus_tag="VN968_21670"
     CDS             9562..9690
                     /locus_tag="VN968_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31879.1"
                     /translation="MKLCTLRTGVMFRINNHSAAGKLVDGGPKRRAPLIGLKKKAV"
     gene            9704..11386
                     /locus_tag="VN968_21675"
     CDS             9704..11386
                     /locus_tag="VN968_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXL31880.1"
                     /translation="MTGDESLQFMDHLRHYRHLTETGGDGARAEQGGPARSHGKLWEL
                     TDLSASEFADEAARFFDLDRIALQDMLSAPPLVAAFSQRFLREMMLFPYQSADGGAVL
                     AVADPTDTAARRAAEIVLRADVAIKVASVEDLTIVLDQRLGGDDLELLGGSEPHQPRE
                     DDIESLRDLASGAPVVRAVNDLIEKAVELRASDIHIEPFHTGLVVRMRIDGLLRPVAA
                     PAGVLPQAVISRIKIVANLNIAERRLPQDGAARLRAGRTDIDIRVAIMPMQHGESAVI
                     RILPKDRGLLVVEKLGFSPADDATLRRLLKLPHGMIVITGPTGSGKTTTLATILSILN
                     EPGRKILTVEDPVEYEIPGVNQAQIKPAIGLTFAAALRSFVRQDPDVIMVGEVRDPET
                     AHVAVHAALTGHLVLTTLHTESAAAAVPRLLDLGVEGYLLRSTLRAVVAQRLVRQLCD
                     RCKMPKTLVEADFAEDPRLGALGLRSGETIQGPCGCERCGGTGYRGRLGVFEILELTN
                     EIRELIGEKTDGLKIDQTAIRAGMTTMVDDGVAKCRAGLTSPAEILRVTTAR"
     gene            11396..12610
                     /locus_tag="VN968_21680"
     CDS             11396..12610
                     /locus_tag="VN968_21680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXL31881.1"
                     /translation="MPNFRYRALTQNGEIVHGTLSAPTAAEVAHRIEYLRLLPIETIE
                     DKTTVAASSSGFGFFGRPSPAEVTTFTRDMALLLKAGARLDDALELLADDADVGRLRP
                     VVGKLRAALLTGESFAGAVADHPALFPAMYVALVRVGEVSGTLDQVLEMLGAERARAE
                     LMRRKLTDAMQYPAFVLIAAACVMLFFILFVLPQFSAVLQDFGAKSDSALSVFIKLSD
                     FLRANAAVMMFASASFVAGVWYLLRRPGAGAAIMTAASGVPGIAGVFQFYRTSLFCRN
                     LGVLLGSGVNLTATLRILVDIMAVTGSVTTWTTAADRVRHGGKLSDALLASSNMPPMA
                     IRMLRLGEETGQLPVLAGRVAEFYEAKLQRSLDRIVGIVGPLAIVTISTVVGGLIVSV
                     MTALLSVTQLVG"
     gene            12625..13122
                     /gene="gspG"
                     /locus_tag="VN968_21685"
     CDS             12625..13122
                     /gene="gspG"
                     /locus_tag="VN968_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HXL31882.1"
                     /translation="MRSAKRVSGFSARRAQGRRQQEFGQQGFTLVEMLVVITIIGLIM
                     GLIGPRVLNYLSESKVKAAKIQLQSFGSALDLFYLDAGRFPSTAEGLAALVRRTPGVA
                     AWNGPYLKGGNVPNDPWNNAYLYRSPGDRGPYDIMSYGSDGQEGGSGLAADISIENMA
                     SAKNE"
     gene            13115..13576
                     /locus_tag="VN968_21690"
     CDS             13115..13576
                     /locus_tag="VN968_21690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXL31883.1"
                     /translation="MSDAERGFTLLEMVCVLALIAMMAAVLLPFIPHNTSRSRLQAYA
                     LQAATLLKADRNAAIRRGADVATLVDAGARSIRSGASADMIRIPDDVRFEALLPQTCN
                     RRAALSTISFFASGMSCGGTIALTRLDAGYEIRVNWLTGRIEIVSRSSATN"
     gene            13548..13961
                     /locus_tag="VN968_21695"
     CDS             13548..13961
                     /locus_tag="VN968_21695"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXL31884.1"
                     /translation="MSPAAPPPIDGRDAGFTIIEVLIALAVVAVSIVAIGSVMSTNVR
                     GVRLLEQHVTLMQTARTVMTAGIPPRAQLGQGASSGQIDDYKWTIDVGPMGEGWDVAG
                     ADVAWIPAIVRIRVRSPSGAVSDLRTVRLMHRPPQ"
     gene            13958..14719
                     /locus_tag="VN968_21700"
     CDS             13958..14719
                     /locus_tag="VN968_21700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXL31885.1"
                     /translation="MSASLARQRAGEGGFTLIETLVAMALMGLVLSALASITAQWLPN
                     WNRGLERIQRSELIGIALQRIGADLAAAEYVPANRDARHPLFEGSELSVTFVRTALGP
                     NAGPGLDVVRVGETTDRQGLVTVRSRASFQPLPLAPSLSEQVHFGEPVLLLRAPFRLS
                     FAYAGQDLIWKSSWHDADRLPAAIKLTVRDAASERVLSISTVAVVHVQSPAQGECAQP
                     DGGCDGKSGAPNNPQGNSQGSSQAGNAATRQGGSR"
     gene            14716..15642
                     /locus_tag="VN968_21705"
     CDS             14716..15642
                     /locus_tag="VN968_21705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HXL31886.1"
                     /translation="MTQNLQPAATASERGFVIVAVLWILAALAALATIFSVYLSNSAQ
                     ALADNDTGLKAEALVSASLELTAYQLLLAGDKARPALGSFHFRLDNADVLVSFTSEAA
                     RIDLNFAPKEMLANLFAGLGADQQAAKGYADRIVGWRTRPTANAANDEEALYAAAGLA
                     YSPRQSLFTHVNELSLVLGLTPVLIDRALPFVTVFSGSSNVDVLVAPPEIIAALPGMT
                     PSALKDFLSQRPALTRDSPAIAAALGPAQATATVPISTSFRILTTVQFDNGRRTSSEV
                     VIALDGNQDPYRVLSWQDEVEAGRRPPKVASD"
     gene            15642..16724
                     /locus_tag="VN968_21710"
     CDS             15642..16724
                     /locus_tag="VN968_21710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXL31887.1"
                     /translation="MAMIPELKALFGEWIAAVATAVNSMIDRYARRRQILLSESGDNT
                     FTAKVTPGRKGAALPDVSFRLSHGRPNPSLPADWQTTFRGSRVEVLLASRQVLFRALD
                     FPKQAVGFLDGMIRAQIDRLMPWAADDVVFGWSPPSAIANERVELTLAAMSKHDAQPL
                     VQLATSLGAASVAAFAVLPDGEGAPVKIKVFDRSLQGVVGRAVDVPRILRVVLLSTGL
                     AAAASLIVAAYIGSALDAEQEQIQRRISQRRAALRLDPNAAGGSALNLLTKRKQTTPS
                     SVMVLEALSQALPDSTYVTELRIEGDKVQVVGMTQDAPSLIRLMERSPQFARATFFAP
                     TTRAQNDPGERFHLEAHITPSFGSGT"
     gene            16721..17278
                     /gene="gspM"
                     /locus_tag="VN968_21715"
     CDS             16721..17278
                     /gene="gspM"
                     /locus_tag="VN968_21715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HXL31888.1"
                     /translation="MNLAAALRKSFAASPILAAAAYAGLVFALLFMVVTSIVDILDQR
                     AAVASAAAMLDQLEGRSTAASGRRSADVLMPSGSAFLEGATVTVAGAALLQRVAGAVT
                     KLGGNVLSSQLDLQGTQSKAGFVSMVASCEIDQPALQPLIYDLEAGMPFLFVEQLAVQ
                     APTSSSGEGKLRILLAVSGQWQGMK"
     gene            17275..17949
                     /locus_tag="VN968_21720"
     CDS             17275..17949
                     /locus_tag="VN968_21720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31889.1"
                     /translation="MMRKLAIAITVVSVYGGASASTSAISNDALDADRAGGAQAGPQA
                     FSISANPAAPVQVLVSSPPPAAPASERALSANPLWAIPLSQLPITRDRPIFSPSRRPP
                     PPAVAPAAIPKVAAVPKPREPERPQLILVGTVASDDEGFGIFLDQSTKVVFRLKVGQD
                     HQGWKLRSVQGREATLEKDQQVVPLALPQLSLGQVTIEVPPPPANAGKLLSVTSQPRP
                     NDRSGR"
     gene            complement(18244..>18444)
                     /locus_tag="VN968_21725"
     CDS             complement(18244..>18444)
                     /locus_tag="VN968_21725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HXL31890.1"
                     /translation="GGFSFGDTNVGLIVSNPRLPSVVLKTPVVTSQVAPTILHALDIE
                     PGALNSVRVERTSTLPGLWNDD"
BASE COUNT         3350 a         5545 c         5933 g         3616 t
ORIGIN      
        1 cggctgttgt cgcaccgggt cacggtaatg aaggcgggtc gcgtcatcga gaccggcctg
       61 accgatcagg tgctcgacga tccccgtgag ccttataccc agttgctcgt ctcttcgatc
      121 ttgccggcat gagggttgcg cggatgactg cgatggtcga tatcagcgac gcagagaaaa
      181 catttaccat gcatttgcaa ggcggggttg agttgccggt ggtgcgtggc gtctcctttc
      241 aggtccgcgc cggcgagtgc gtggtgctgt ccggtccctc gggagccggc aaatcctcga
      301 tcctgaaaat gatcttcggc aattaccgct gcgatgccgg ccgtatcggt atttgtcacg
      361 aaggtgtgca gatcgacctc gccaaagccg agccgcggca gatcctcagc gtccgccgcg
      421 ccaccatcgg ctatgtcagc cagttcctgc gtgcggtgcc gcgggttgcg gcgatcgacg
      481 tcgtggccga gcccttgatc gcaaatggcg cggcaagggg gcacgcgcgc gaacaggccg
      541 ggagcctgtt gcgggctctc aacattcccc aacgtctgtg ggcgctgccg ccttcgacat
      601 tctcgggagg cgagcagcag cgcgtcaaca tcgcgcgcgg ttttatttcc gatctgccga
      661 tcctgctact cgacgagccg acggcttcgc tcgatgccgc caatcgcgcc gtggtggtgg
      721 atctgatcaa tgaaaagaag cgtgcgggcg ttgccatggt cgccatcgtc catgacgatg
      781 agatccgcca tctgatcgcc gaccgggttg tcgacgtaac atcgtttgcc gcagcggcat
      841 gaaggaagac gcttaagatg acctcgacaa gggacgagac cgtgcttggc aacgccagga
      901 ttgtcctggc ggaccgcgtg atcgagcgcg gctggatcgc cttcgccgac ggccgcgtcg
      961 ccgaagttgg cgagggtgac ggacctgtgg gcagccagga cgcagccggc gacctgatca
     1021 tgcccggcct gattgagcta cataccgacc atctcgaagc acattatgtg ccgcgcccaa
     1081 aggtgttctg ggacccgatc gccgcggtcg tttcctatga cgggcaactg gcgacatcgg
     1141 gcatcaccac ggtactggat tcgctccggg tctggcgcga ggacggcgcg gaagaagtcg
     1201 acggacgggc aggcatactg gccgaggcga tcgcatcggc gcgcgacgcc gatctgttgc
     1261 gtgccgatca tttcctgcat ctgcgctgtg aaattccgat gccgagcgtg gtcgaggaag
     1321 ccagtgaact ggtggggcgg ccggatgtgc ggttgatgtc gctgatggac cacacccccg
     1381 gacagcgcca gtttcgcgac gaagacaagt tgcgcaccta ttatcgcggc aagggtgccg
     1441 gtatgaccga cgccgaactc gatacgctgt tcgaacggcg ctttacgtat cagaaggcgc
     1501 atgcggcgac caacatgcgc gagatcgtgg cgctggcgca gcaatatgaa atcccgctcg
     1561 ccagtcacga cgatacaaca gacgaaaacg tcacggacgc cattcgcgac cgggtttcgg
     1621 tggcggaatt tccgaccacg atcgatgccg cgcggggatt gcaccgggcc ggcatcggca
     1681 tcctgatggg agcgccgaat gtggtccgtg gcggttcgca ttccggcaat attgccgcgg
     1741 tcgatctcgc ccgcgagggg ctgctggata tcctgtcgtc cgactatatt ccgtcgagct
     1801 tgctgatggc ggcgctgcaa ttgcctcagc gggtgccggc catcgatctc gcctccgccg
     1861 tccgcacggt caccaagaca ccggccgaag cggtcggctt gaccgatcgc ggcgcgatcg
     1921 cggtgggcaa gcgcgccgat ctgattcggg tgcatattgc ccgcgacatt ccggttgtgc
     1981 gcagcgtctg gcgcgaagga catcgcgtgg cgtgacggaa gcctcagcca aatcggatca
     2041 tccgccggct gcgatcgggc ctggacggct cattctggtg gtcggtccga gcggcgccgg
     2101 gaaggacacg ctgatcggat gggccgcagc cgcctgtgcc aacgacggca atatcgtgtt
     2161 tccacgtcgc gtggtgacgc gggaagcctc ggcatccgaa gacaatgaac aggtgagcct
     2221 ggatgccttc ctgcatgcgc gcagcaggga cgagttcgcc gttcaatggg aggcgcatgg
     2281 ccattgctac gcactgcccc gcgcgatcaa tgacgacatc cgtggcgggc gcacggtggt
     2341 cgccaatgtg tcgcgcacgg tggtcgacgc agtgcgtcgc gcctacgccg cggtcacggt
     2401 ggtgtcgatc accgcgcccc ctgacattct cgccgaacgc ctcgccgcgc gggcccggag
     2461 cagcgatggg cagcttgcgg atcgactgcg tcgcgccgtc gatgatgccg cggctgtgcc
     2521 cgacgtcacc attatcaacg taggcagcgt cgaagatcgc gcgcgcgagt tcgtgcaggt
     2581 catcaggaaa gcttgacgta agcgggcggt gcgcggccgg ccgcggagat caggagaacg
     2641 acaatgacga tcattgcgac gattggcgag cttgaagcga tctatggaca gccgaacgag
     2701 gcgtctaccg tcaaggtcgc tgaccgaata acgccgcaat atcgcgtgct gatcgacaaa
     2761 tcgccgtttg ccgcgctggc gacctgcgga ccggagggac tggattgctc gcctcgtggc
     2821 gatttgcccg gcttcgtccg tgcgcacgac gaaacgacgc tgatgatgcc cgatcgccgc
     2881 ggcaacaatc gcgtggattc gctgcgaaat atcgtacgcg atccccggat cgcgctgctg
     2941 tttctgattc cgggttccgg cagcacgttg cgcgtcaatg gccgtgcaag ggtgtcagcc
     3001 gatgcggact tgctggcttc cttcaaggtc gacggcaagg cgccgcgcac ggtcatcgtc
     3061 atgacggtcg atgagatcta ttttcagtgc gcccgcgcga tcgtccggtc cgatctctgg
     3121 aatcccgaca ggcgggtcga tcccaaaagc ttgccgacgc ccggtcagat cctcgccgag
     3181 atgagcgaaa atcgtgtcgg cggtgaggac tacgatcggg cctggccgga gcgggcgcgg
     3241 caaacgatgt ggtagcggac cgctgggatc gcggcggatc gcccggaggt cgaactcgct
     3301 accgatcact tccggtggcg attccttatg aatttccggg gtaacgggaa cattcgcgag
     3361 aagacagccg cgtcaagcgt tgccgggcgt gtccggctga atacgaaaat atccatcacg
     3421 cgcaaaagtg ccggcaacat tgagatgtcg ctcagaaaag agactgataa ctgaggctcg
     3481 tccttgctag cgccgcattg acttaggaca atgcgtacgt cgtcaaaatg cgcctagact
     3541 gattccatga aaatatccga ctttgcttgt ccgtcctgcg ccgcgtccta cgaggttgcc
     3601 gagtctatgt cggcggaagg aagcccaggc cgggccgaat gcaccatatg cggccagctt
     3661 ctggaatcgt ggcaggaacc caagatgaaa gcctatcgct tggtcttgtc gccggaacat
     3721 aaatatccgc acattccagc gccgccgtct ccgattgtca cctgatttca aagccgggca
     3781 tcgctctatt ccgaaatagg gctcggggcc cgatctcgac aatccggcgc ccggattggc
     3841 gcgtgcccac gaaataattt tcttcgtgca gccgctcgct atgaagtccg cgacgcggat
     3901 tgacggaatg tcgcgacaat ttcggtcggg ctgcaaagat ctacctgcga aaaaacccgc
     3961 cggcttgcac cggcggtttt tgggttgttc aatttccgaa aacttaattc tcggcgcgat
     4021 ggtcgtgatc cccgtcgcga tggtcgtgtt cgcccttttc ggccaggtcc ttgaccaggt
     4081 cgatcaaggc tttggcgcgg tggccgagac catcttccgc accctcctcg accgcctggt
     4141 tgtggaatgc cgcattgttc aacaccgtct tgatctggct cgcaagttgg tcgcgatcgc
     4201 cggtgatatc gtcgatccgc ttcaaataac gggcatagac cttgtcggtg ctggtgaccg
     4261 agcggttaga ccaaaccaga ctggcgcggc cgagctcacc ctttggcgcg ttgagctgct
     4321 tgaacacctg agccaactcg acaaagtttt cgcggctctt gcggataccg ggcggcagcg
     4381 cattgtcctg catgaactcg gcgacgacgc ggccctcatg cacatagtcg tccgtcaggc
     4441 ggagcagcgc catgagcgtc ggccgtacgt cggcatggtc ggtgaacacg tcgtcgtcgc
     4501 ggccgagccg cttcacaccg ggtcccacca tggcaaccca ggtctgggtg atctgtttct
     4561 ggaagtcgcc gtggttccag gcaaagccgg gagcctcgca cacgacgtcg gtcggggtcg
     4621 tgcagccgtt gttgttgcct gtttcgaaga aatagtcgtc gttgccgaag atggtgaacg
     4681 tcggagtgcg cgccgggctc ttggttacca tgtgcaggag cttcatttcg gcccggtcgg
     4741 ccagcagctt agacagcttg tcgttgttgc cagtgatcgg actggtggcg accagtgcgt
     4801 cgacgtcatg ctccatggta cgggttagcg tgtcggtcgg ccccggattg gtgttgatgt
     4861 acatggtggg agcgtcatcg ctgtgcaccg agaacggcgt gctattcatt cgctgagcga
     4921 gcaagagctt atctagcgac gcctcgatct cgccgacctt ggtataagtg caaggcgtgg
     4981 taatgccgtc gcaattggcc ggggtgggtg ctccgccgac aaaatggtcg ttctcgtccg
     5041 gcacggcgac aaacagcgtg ttggtctcgt cgatgccatc cgctttgagg cgggcgaaga
     5101 atttgccaaa ggccgtatcg taagccttaa gcgccgcaac ataaccagcc tcgcctggcc
     5161 cataggcatg ggagctgttc ggagtgggac ccgaattcgg aatgtcctga cgatgcggat
     5221 cgtgcgcatc ggcaacataa gtgtaaacga cgggaacgcc agcttcgagc attttagcgg
     5281 cgtagccgag cgaggctgtc gcggaaggac tgaagtcagt ggggaagccc ggcttattcg
     5341 agaagggatc ggtgatcacc acgttatcga gatcggtcac cggaccgctc ggcgagatca
     5401 ccggctgcac gttgatgttg ccgtaaagag cagagaagcc gttataaccg ccgggctcat
     5461 cgggaagaag atcgggcctc gcaaaggagg ggttgttgca caggccgctg cccggcgcgc
     5521 agtgaatcgc aatgccttca tagtcttggg ttgcggcgcc agcagcgacc gacgaaccaa
     5581 agatgtttgc aatatccgtc ttgttttcta ttgcaagacc attcaaggag acagcgccca
     5641 cgtcgcagcc agcgcgggtg tagggaaccc acggcgccgg cgcgttcttt ccgttctcat
     5701 tcaagaggac gggcagattt tcgccggctt cgggatggct ctgcgccgtg gtggtcgtcc
     5761 agtaaacgaa ggaagtttgg aaaacggccg cggaattgtc cttcttaaag atcccgaagc
     5821 tgttcgagac cgggctgccg tgacgatcgc catagacccc cgtcaacccc gtgagaatat
     5881 cgtcggccgt gtgggagatc agcggcgtat aatgattgcc gctgatgacg cctcttcctt
     5941 gcaggaaatt caagaggttc ggcatctgct cgaggtcaga tggaacgttt ggattgtcgc
     6001 gccgcaaatg cacgttgtcg aacacgatat gaacaacatg tttgacctga ccgcctggag
     6061 aattcagtag gcaagccgcc gaggcggatt gcgaagcggc gccgagaaat accgcgccaa
     6121 gcgccaacgg aatcgaggcg ccaagaaaat ccttcaatcg tttcatcgct ctctcctgag
     6181 gtcggcccaa gtggagtaaa tctgtgaaaa aaccgcgcag tctagttgcc gacggtggca
     6241 caatacattg cagattgttg cgggtgattg acgcgaacat gacgctcgcg cgcggcgtga
     6301 ggtcatgcga tcggaggagc ggctctcacc gaggagattt tggttggtga taaagagtcc
     6361 gaccgaacac atggaatacg atcgcgagcc aatccggcgc gatcgcgcca acgcgtcatt
     6421 ctcgtcatgt cggaagcgca gtggcggtca ccagcgtcct ggtgcccaga acaaagcaag
     6481 ccgctggcct tcaacgatct gatcggacga cattttcgaa gccaccgcgt tgcgaagcct
     6541 gagataatag tcgcgctcgc gcctgggggc atgtgccgcg gccaggtcga gccatttata
     6601 tgcgagcacg acgctttggg gcacgccgtg acccttgtca taactcaggc ctaatctgct
     6661 ctgtgcagag ggatcgccct gcacggctgc tttctggtag agatcggtgg cggcgtcgta
     6721 ggcctgcggc accccgaagc cgttttcgta catgaagccc agcagcgtct gcgcccgggc
     6781 gtttccgcgc agcgccagcg gggataattc gttggcggcc cgcacgtaat cgcccctgga
     6841 ataggctgca gtcgcgcggc tcagtccgtc ggcgaaggcg gcccgcgtcg ttcctgcccc
     6901 aaccagcaag atgacgaata gaagcgccgg tatggtccgt cgtactgcgg ctgccttcag
     6961 gagcgctgaa caaagtgccg ctgaggccga gcccgggatt tgcgtcctgg cgtcagcgaa
     7021 cgccctgtac tcgtgtgtca tttgccacgc tcgcgttgat gctgccgcgg agttttgagc
     7081 ggagttcttc ggcaacgaaa tgggcatcca tgctgtcgcg gatgatctgg gggcggataa
     7141 agatgatcaa ttcggtacgc gtcgccttat tgtcttgatg agagaaggcg tcgcccagac
     7201 cttgaatttg gtcgagcagg ggaattccgt tgcgctggtt gttgtgttgt tcgctgatca
     7261 atccggccaa cagcaccgtc tggccggtgg ctacggaaat cgagctcttg acctttcgct
     7321 cggagaccgt cggagtcaga ctcgcggcgg tagccgccga aacgttgctg atttcctgct
     7381 cgacgtcgag cctgacattt ccgttgacgt tgatgcgggg agaaacccgc agaatgatac
     7441 ccgtgttgcg gtagtcgatg gtgttgacga ccgtgttgtt cgtggtcagc accgtagcgc
     7501 tgccggtgga gaccggcacc gagtcgccga cctgaagtgt tgccgcctga ttgttgacga
     7561 caaccagcga cgggttggac aacaccttga cggcggtgac ggtatgcagg gcgtccagga
     7621 ttgcgctcgg ctgcgcttcc gatccgatca ggaaattgaa tccggggaac gcgcgattga
     7681 tgaaggcgtt ggcgaccgta ccggcggccg cggcgaccgg tgcggtcgtc gactgggtgt
     7741 tgaggatgga gccctggtcg ggccttagtc ccagattgtg gctggtcagg tagctctgca
     7801 cgccatagct cagctcgtcg gtgagcgtga tctcggcgat ggtcgcatcg atcgcgacct
     7861 gcagctgtgg ttgatccacc tgctgcaacg tcgcttcgat gatccggaaa ttctcctgat
     7921 ctgcataaat gagcaggctg ttgttgacgg tatcggccgt gatccgcacg cccttcaaca
     7981 tcggctggcc gccggagccg gcgcctcggc tgtcgagcga tccggcgtca gacgtatttg
     8041 cgtttgcgcc tgccggcgcg gcggtggttt gcgcgccgaa gccggagttg ccgttggttc
     8101 cggattgcat gcgcgagccg aagccgctcg acgacgttga cgacggagag ttgctgttta
     8161 gggagaggcg atcggcgctg gcggtcgcat atgtgcccga ccccggcgcg atctggctgt
     8221 cggggctgtc gagcaggccg gaggacgatc cgccgatgaa catctcggtc agcacgcgcg
     8281 cgatctgacg cgcctcgcca tatttcaccc ggtagacgtg aacgctggtt cgcgcagtgt
     8341 cggcatgatc gagccgcttg atccaggtcg cggcggtgtg cagcagtccc ggcttcttgc
     8401 tcaccaccag gatggcgttg aggcgactaa ccgcctgaaa cttgatgaga ttttggctga
     8461 gaccgttgtc gccggaatca acgatctttt cgagttccgc gattacgggt tccggaccgc
     8521 tgttgacgat cggaaaaatt ccgatcgatt gaccgcgcat ccagtcgagg tcgaagctga
     8581 gcgcggtatc caccgccgtg cgacgttccg cgccgctgcc ctgaatcaga agcaggtttc
     8641 gcgtcgtgtc ggcgcggacc attccggcct tggtcgcgaa actgtccatc aacttcagca
     8701 gcgtttgcgc cgacacgtac tgcaagggca cgacggagac gccgtagcca ggttcagggc
     8761 tggaggccgc cgcatcgatc cggcccgcgc cgaccgcgtc gccgagcggc gtcaaacggt
     8821 agccggcggt gtcgcgaacc agcacgatcc cactgagccg taacgcattt tcaagcacga
     8881 agacgatgtc cgacttggcc accggcctga ccgatacaag gctgacggtg ccctgcacgc
     8941 gcggatcaat cgtgtaaccg gtttgcagaa tgtcgcccag caccactttg gccacggtgg
     9001 cgaccggtgt gttttcaaaa ttgagctcaa agccgttgcc ggtcgcgacc ggttgtggac
     9061 gcgtctcggc gacgtcggaa acctcggtgc cttcgaacat caccggccgg ctgcgctggc
     9121 cgacattggc ctcagcggcg ttcgcctgct gtggataacg aggcaaggta tccagcgatc
     9181 ggaccttgtc gagcacgtcg atatcggcgc cgctgccgga gcccatagtc accgcattgc
     9241 aggatgccag caggccggcg gacaacagca taaaggcaat agaaatgctg cgaccgactg
     9301 gcggagtcca gccctggttg gcgacgcgta ccatatttcc gccgttccag cgttatgttc
     9361 ggcccaaaga tcgctcggga ggattgatcc aagctggctg gcatacttag cggcggaaca
     9421 tgacaattgt aattgccggc ccggtcgatc gggcagatct acgtcacctg tggccaacat
     9481 ccaacacatt gcgttcgcgc gcgatgcgct ccccccgccc cctgcggccg ggtccaatga
     9541 acaggcaaag gctaaattga cgtgaagctg tgcacgcttc gtacaggtgt catgtttcgg
     9601 atcaataacc acagtgcggc cggcaagctg gtcgacggcg ggccgaagcg ccgtgctcca
     9661 ctgatcggcc tgaagaaaaa agctgtgtga gtggaaagac ataatgaccg gcgacgaatc
     9721 cttgcagttc atggaccatc tccgtcatta tcgtcatttg acggagaccg gcggtgacgg
     9781 cgcgcgcgcc gagcagggcg gcccggcgcg ttcgcacggc aaactttggg aactcaccga
     9841 cctctcggcc agcgagttcg cggatgaggc ggcccgcttt ttcgatctcg atcggatcgc
     9901 gctccaggat atgctgtccg cgccaccctt ggtcgcggca ttttcgcagc ggtttttgcg
     9961 ggaaatgatg ctgtttccat atcaatccgc cgacggaggc gccgtgctgg cggtcgccga
    10021 tccgaccgat acggcggccc ggcgcgcagc cgaaatcgtg ctccgggccg acgtcgcgat
    10081 caaggtggcg tcggtcgaag atctcacgat cgtcctcgat cagcgtctcg gtggcgacga
    10141 tctcgaattg ctgggcggca gcgaaccgca tcagccacgg gaagacgata tcgaaagcct
    10201 gcgcgacctt gccagcgggg cgccggttgt tcgagccgtc aatgatctca tcgagaaagc
    10261 tgtagaactg cgcgccagcg acattcacat tgagcccttt cataccgggc tggtggtccg
    10321 catgcggatt gacggcctgc tgaggccggt ggcggcgccg gccggcgtct tgccgcaagc
    10381 cgtgatttcg cgcatcaaga tcgtcgccaa cctcaacatc gcggagcgtc ggcttccgca
    10441 ggatggcgcg gcgcggctgc gggccgggcg taccgatatc gacattcgcg tcgcgatcat
    10501 gccgatgcag catggggaat ccgcggtcat acgcatcctg ccgaaggatc gcgggctgct
    10561 ggtggtcgaa aagttgggat tttcgccggc cgacgatgcc acgctcagac gattgctgaa
    10621 gctgcctcat ggcatgatcg tcatcaccgg cccgaccggc agcggaaaaa ccacgacatt
    10681 ggcgacgatc ctgtcgatcc tgaacgaacc gggtcgaaaa atcctcacgg tcgaggatcc
    10741 ggtcgaatac gagatacccg gcgtcaacca ggcacagatc aagcccgcga tcggactgac
    10801 gtttgcagcg gcgctgcgtt cattcgtgcg tcaggatccg gacgtgatca tggtcggcga
    10861 ggtgcgcgac cccgaaaccg ctcacgtcgc cgttcacgcc gccctcaccg gtcacctcgt
    10921 tctgacgaca ctccacaccg aatcggcggc cgccgcggtc ccgcggcttc tcgatctcgg
    10981 cgtcgaggga tatctgctgc ggtccacctt gcgagcggtg gtcgcccagc ggctggttcg
    11041 ccagctgtgc gaccgttgca aaatgccaaa gacgctcgtc gaggcggatt ttgccgaaga
    11101 ccctcgattg ggcgcgctcg gcttgcggag cggcgagacc attcagggcc cctgcgggtg
    11161 cgagcgctgc ggcggtaccg gctatcgcgg ccggctcggc gtgttcgaga tccttgaact
    11221 caccaacgag attcgcgaac tgatcgggga aaagaccgat ggactgaaaa tcgatcagac
    11281 cgcgatccgc gccggaatga cgacgatggt cgacgacggc gttgcgaaat gccgcgccgg
    11341 cctgacttcg cccgcggaaa tactccgcgt tacgactgcg cggtgaggtg agatcgtgcc
    11401 gaatttccgc taccgggccc tgacgcagaa cggagagatc gtgcatggca cgctatcggc
    11461 tccgacggcg gcggaagttg ctcaccgcat cgagtatctg cggctgctgc cgatcgaaac
    11521 catcgaagac aagacaaccg ttgccgcttc aagctccggt ttcggtttct tcggccggcc
    11581 gagcccggcg gaagtaacga cgttcacccg ggacatggcc ctgctgttga aggcgggcgc
    11641 gcgcctggac gacgccctcg aattgctcgc tgatgacgcc gatgtcggcc ggcttcgtcc
    11701 ggtggtcggc aaattgcggg cggcacttct gaccggagaa agtttcgccg gcgctgttgc
    11761 ggatcacccg gctttgtttc ccgccatgta cgtcgcgctg gttcgcgtcg gcgaagtatc
    11821 gggcacgctc gatcaggtgc tcgagatgct gggggcggag cgggcgcggg ccgaactgat
    11881 gcgccgcaaa ctcaccgatg caatgcagta cccggccttc gttctgattg ccgcggcctg
    11941 cgtcatgctg ttcttcattt tgttcgtact gccgcagttt tctgcggtgc tgcaggactt
    12001 cggtgcaaaa tcggattccg cgctcagcgt tttcatcaag ctgtcggact ttcttagggc
    12061 gaacgcggcc gtcatgatgt ttgcctccgc atcgtttgtg gccggcgtct ggtacctgct
    12121 gcgccgcccg ggcgcaggcg ccgcgatcat gaccgcggcc tctggcgttc ccggtatcgc
    12181 cggcgtcttt caattttacc gcaccagtct gttttgccgg aatctcggcg tgctgctcgg
    12241 tagcggcgtc aacctcacgg ctactttgcg catcctggtc gacatcatgg cagtcactgg
    12301 aagcgttacg acctggacga cggcagccga tcgcgtccgt cacggcggca agctttcgga
    12361 cgctctgctc gcctcaagca acatgccgcc gatggccatc cgcatgctgc ggctgggtga
    12421 ggaaaccggg caattgccgg tactggcggg ccgggtcgcc gaattctatg aggcgaaatt
    12481 gcaacgcagc cttgaccgca tcgtcggcat cgtcggcccg cttgccatcg tcacgatcag
    12541 cacagtggtc gggggattga tcgtatcagt catgaccgcg cttctgtcgg ttacgcaact
    12601 tgtcggatag agggttggtt catcatgaga tcggcaaaac gtgttagcgg gttttccgcc
    12661 cggcgcgcgc aaggcaggcg tcagcaggag tttggccagc agggtttcac gctggtcgag
    12721 atgctggtgg tgatcaccat catcggcctg atcatgggcc tgatcggccc gcgcgttctc
    12781 aattatctca gcgaatcgaa agtcaaggcc gccaaaatcc agctgcagag ttttggcagc
    12841 gcgctcgatc tgttctatct cgacgcgggc cggtttccct cgaccgcgga gggccttgcg
    12901 gcgctggtgc gccggacacc gggtgtagcc gcctggaacg gaccgtatct caagggcggc
    12961 aacgttccca acgatccctg gaacaacgcc tatctatatc gctcgcccgg agaccgcggc
    13021 ccttacgata tcatgtccta tggatcggat ggccaggaag gcggcagcgg tctggcggcc
    13081 gacatctcca tcgaaaacat ggccagcgcg aaaaatgagt gacgcggaac gcggcttcac
    13141 gttgcttgag atggtgtgtg tgctcgcgct catcgccatg atggcggctg tgctgctgcc
    13201 gttcataccg cacaatacct cccgctcccg cctgcaagcc tatgcgcttc aggccgcgac
    13261 gcttctgaag gcggaccgca acgcggcgat tcggcgcggg gcggacgtcg ccacgctggt
    13321 cgatgctgga gcgcgctcga tccggtcggg cgcgagtgcc gatatgatcc gtattcccga
    13381 cgacgtccgc ttcgaggcgc tattgccgca aacctgcaac cggcgtgcgg ccttgtcgac
    13441 tatcagcttc tttgccagtg gaatgtcgtg cggcggaacc atcgcgctga cccggctcga
    13501 tgccggctac gaaattcgcg tcaactggct gaccggaagg atcgaaattg tctcccgcag
    13561 ctccgccacc aattgacggt cgcgacgccg gcttcacgat tatcgaagtg ttgatcgcgc
    13621 tggcggtcgt tgccgtctcc atcgtggcga ttggatctgt catgtcgaca aacgtacgcg
    13681 gcgtgcgctt gctcgagcag cacgtgacat tgatgcaaac cgcacgcacg gtcatgactg
    13741 ccggcatccc gccgcgcgcc cagcttggcc agggcgcgtc gtcgggacag atcgacgact
    13801 acaagtggac gatcgacgtc ggaccgatgg gcgagggttg ggacgtggcc ggcgcggacg
    13861 tcgcctggat tcccgcaatc gtcagaattc gtgtccgatc cccctccggc gcggtgtccg
    13921 accttcgcac cgttcgcctg atgcacaggc caccgcaatg agcgcttcgc ttgcaaggca
    13981 acgggccggc gagggcggct tcacgctgat cgagaccctc gtggcgatgg cgttgatggg
    14041 cctggtgctc tcggcgctgg ccagcattac cgcgcaatgg cttccaaact ggaatcgcgg
    14101 cctggagcgc atccagcgca gtgaattgat cggcatcgcg ctgcagcgga ttggcgccga
    14161 tcttgcggcc gccgaatatg tgccggccaa tcgcgacgcg cgccatccgt tgttcgaggg
    14221 ctcggagctg tcggtgacgt tcgtgcggac ggcgctgggc ccgaacgccg ggcccgggct
    14281 ggatgtggtg cgggtcggcg aaaccaccga tcgtcagggc ctcgtgacgg tccgctcgcg
    14341 ggcgtcattc cagccattgc cgctggcacc gtcgctatca gaacaagttc atttcggcga
    14401 accggtgttg ctgttgcgtg cgccgtttcg gctgtcattc gcctatgcag gccaggacct
    14461 catctggaag agcagctggc atgatgccga cagactgccg gcggccatca agctgacggt
    14521 gcgcgacgcg gcgagcgaac gagtcctctc gatttcaacg gtagcggtgg ttcacgtcca
    14581 atcgccggcg caaggcgagt gcgcccagcc ggacggcggc tgtgacggca aatcaggcgc
    14641 tccgaacaac cctcaaggaa attcgcaagg aagttcacag gccggaaacg ctgcaacacg
    14701 gcagggagga tcgcggtgac gcaaaatctt cagccggctg caacagcctc agagcgcggc
    14761 ttcgtgatcg tcgctgtgct gtggattctc gcggccctgg cggcgctggc gacgatcttc
    14821 tcagtctatc tgtccaattc ggcgcaggcg ttggccgaca atgataccgg actgaaggcg
    14881 gaggccctgg tgtcggccag cctcgaactc acggcctatc aattactgct ggcgggcgac
    14941 aaggcgcgtc ccgcgctggg gtcgtttcac ttccggttgg acaatgcgga cgtccttgtc
    15001 agctttacct cggaagccgc ccgcatcgat ctcaacttcg cgcccaagga gatgctggcc
    15061 aatctatttg caggtctcgg tgccgatcag caggccgcca agggatatgc cgaccggatc
    15121 gtcgggtggc gaacgcgtcc cacggccaac gcggccaacg acgaggaggc gctctatgcc
    15181 gccgcgggcc tcgcttattc tccacggcag tcgctcttta cccatgtgaa tgagctcagt
    15241 ctcgttctcg gcctgactcc ggtcctgatc gatcgcgctt tgccgttcgt gacggtcttt
    15301 agcggatcgt ccaacgtcga tgtgctggtc gcgccgccgg agatcatcgc cgcattgccc
    15361 ggcatgacgc cctccgctct gaaggatttc ctgagccagc ggccggcgtt gacgagagac
    15421 tcacccgcga tcgcagccgc gcttggcccg gcgcaggcca ctgcgacggt ccccatcagt
    15481 acgtcctttc gcatcctgac gacggtccag ttcgacaatg gccgccgaac ctcgtcggag
    15541 gtcgtgatcg cgctggacgg gaaccaggat ccttatcgcg tgctgtcgtg gcaggatgaa
    15601 gtggaggctg gccgtcggcc gccgaaagtg gcgagtgatt gatggcgatg atccctgaac
    15661 tcaaagcgct gtttggcgaa tggatcgcgg ccgttgccac ggccgtcaat tccatgatcg
    15721 atcgatatgc gcgccggcgg cagatcctgc tcagtgagag cggcgacaac acgttcacgg
    15781 ccaaggtgac gcctgggcgg aaaggcgccg cgctgccgga cgtttcgttt cgcctctcgc
    15841 atgggcggcc caatccctcc ctgcctgcgg attggcagac gacatttcgc ggcagccggg
    15901 ttgaagtcct gctggcgtcc cgccaggtgc tgtttcgcgc gctcgacttt cccaagcagg
    15961 cggtgggttt tctcgatggc atgatccgcg cgcagatcga ccgtctgatg ccctgggcgg
    16021 cagatgacgt ggttttcggt tggagtccgc cctcggcgat tgcgaatgag cgggttgagc
    16081 tgacgctggc cgcgatgtcg aaacatgacg cccagcctct cgttcaactg gcgaccagcc
    16141 tgggggccgc atcggttgcc gcgttcgccg tgcttccaga cggcgagggc gcgcccgtga
    16201 agatcaaagt cttcgacagg tcgctgcagg gcgtggtcgg acgggcggta gatgtgccgc
    16261 gcattctgcg cgtggttttg ctgtcaaccg gccttgcggc cgccgcctcc ctgatcgttg
    16321 cggcttacat cgggagcgct ctcgatgccg agcaggaaca aatacagcgc cggatttcgc
    16381 aacgccgcgc ggcgctgcga ttagacccga atgccgcggg cgggtcggcc ttgaacctgc
    16441 tgacgaaacg caagcagacg acgccatcca gcgttatggt gcttgaggcc ctctcgcagg
    16501 cgctgcccga cagcacctac gtgacggagt tgcggatcga aggcgacaag gttcaggttg
    16561 tcggcatgac gcaggatgcg ccgtctttga tccgattgat ggagaggtcg ccgcaatttg
    16621 cgcgcgccac tttctttgcg cctaccacgc gcgcgcagaa tgacccggga gagcgtttcc
    16681 acctcgaggc ccacatcacg ccgtctttcg gatcgggcac atgaacctcg cggctgctct
    16741 gagaaaatcg ttcgcggcgt cgccgatcct ggcggcggca gcctacgccg gactcgtgtt
    16801 cgcgctgctg ttcatggttg tcacctccat tgtcgacatt ctcgaccagc gggcagcggt
    16861 ggcatcggcg gcggcgatgc tggatcaact cgagggccgc agcaccgcag catccggcag
    16921 gcgatccgcc gacgtcctga tgccgtccgg ttcggcgttt ctggagggcg cgacggtgac
    16981 cgtcgcgggt gcggccctgc tgcagcgagt cgccggcgcg gtgacgaaac tcggcggcaa
    17041 tgttctgtca tcccagctcg atttgcaggg cacgcaatcc aaggcgggat ttgtcagcat
    17101 ggtcgcgagc tgcgaaatcg atcagccggc attgcagccg ctgatctacg atctcgaagc
    17161 cggaatgccg ttcttgtttg tcgaacagct cgccgttcaa gccccgacct cctcgtccgg
    17221 tgaggggaaa ttgcgaattc tgctggcggt atcggggcaa tggcagggca tgaaatgatg
    17281 cggaagcttg cgatcgcgat cacggttgtg tcggtgtacg gcggcgcctc cgcatcgaca
    17341 tcagctattt ccaacgatgc gctggacgcc gatcgggccg gtggcgcgca agcggggcct
    17401 caggcattct cgatcagcgc aaatccggcc gcgccggtgc aggtcctcgt ttcttcaccc
    17461 ccaccggccg ccccggcatc ggagcgggcg ctgagcgcca atccgttgtg ggcgatcccg
    17521 ctctcgcaat tgcccatcac gcgcgaccgg ccgatctttt caccgtcccg gcggccgccg
    17581 ccgccagcgg tcgcgccggc cgcgatcccg aaagtggcag ccgtgcccaa accaagggaa
    17641 cccgagcgtc cgcagctcat tctggtcggc accgtcgcaa gcgacgatga ggggttcggg
    17701 atatttctgg atcaatcgac caaggtggta ttccggctga aggtcggcca ggaccatcag
    17761 ggctggaagc tgcgatcggt ccaaggccgt gaagctacac tggaaaagga tcaacaggtt
    17821 gttcccctcg cattgccgca gctcagcctg ggacaggtga ccattgaagt gcctccgcca
    17881 ccggcgaacg ccggaaagct gctgtcggtg acgtctcagc cgcgacccaa tgataggtcc
    17941 ggtcgctaat gtcctgtgat cctctggtcg ctcagcacca cgtctagtgt tgttgaggcg
    18001 ctttcagcgc ggcaaccatc acaagcatgc gcaggccccc gatgcctaaa aattcgttgg
    18061 cttcgcgcgg gatgcgcgcc ggaaccgaag agagaggaag agggcgttcc gaaagccgat
    18121 gctcttaccc gtcccttaag gacaatcatc aagcgggatg tcattgccgt gtaactggtt
    18181 tgcgaagcgg aaggggccct cgaagagggc cctttctgaa ccagacgttg ccgagtacca
    18241 agtctagtcg tcattccaca aaccgggcag cgttgaagtg cgctctactc gcacagagtt
    18301 caaagctccg ggctcaatgt cgagcgcatg caggatggtc ggtgcgacct gcgaagtgac
    18361 cacaggggtt ttcaggacga cgctgggaag ccgcggattg gaaacgatca acccgacatt
    18421 ggtgtcaccg aagctgaaac cgcc
//
LOCUS       DATNOI010000535         6988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2169234,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000535 DATNOI010000000
VERSION     DATNOI010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2169234"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VN968_21730"
     CDS             complement(<1..623)
                     /locus_tag="VN968_21730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31891.1"
                     /translation="MTTSQIFSKWDETHSELWSHQPIRLEHEMHNSPAFSMDDLAKLI
                     EGYPRQHYSIVQTGARGSSRVWREGDLGNLRGSEVIDAISRGELWLNLREVGSVDSRY
                     RQLVDRMFEEIAAKVPGFEVPKDHQEGILISSPRAEVYYHADLPSQSLVQIAGRKRVY
                     VYPNTAPFITPQHLEDITLFNVEVDLPYKAWYDDHAKVLDIGPGQML"
     gene            1123..1317
                     /locus_tag="VN968_21735"
     CDS             1123..1317
                     /locus_tag="VN968_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31892.1"
                     /translation="MNTPDQETVLRAMEDARRILAEYIAPGQHDAMRTVERLLTVLDK
                     SDVVHALDRMKRCRVQRLVD"
     gene            complement(1671..1855)
                     /locus_tag="VN968_21740"
                     /pseudo
     CDS             complement(1671..1855)
                     /locus_tag="VN968_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846162.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
     gene            1940..2407
                     /locus_tag="VN968_21745"
     CDS             1940..2407
                     /locus_tag="VN968_21745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL31893.1"
                     /translation="MKATKRFWRYLAMPLALVGMLSLSSAAELNPAAVIYKLPDQIPW
                     SPVDARGGQNAVVVGDPAKPGFYAVYTKWTKGNHFSRPHFHPNDRYIAVLQGTWWVGS
                     GPKFDPANTTAMPAGSFVTHFGKQVHWDGAKDEDAVLLIMGEGPATSTAAEEK"
     gene            2507..4039
                     /locus_tag="VN968_21750"
     CDS             2507..4039
                     /locus_tag="VN968_21750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL31894.1"
                     /translation="MIGFTRPPCDAGIIRATSAAPAALSRRRKQMTLAATILGSSMAF
                     IDGSVVNIALPGIQQALQANAASTQWIVNGYLLLLGALVLVGGSAADLYGRRRIFLVG
                     IAIFTGASIACGLSPDITVLVVSRAVQGLGAALLIPASLAMLGATFDDHERSHAIGTW
                     AGVGALTAAAGPVLGGWLVDQVSWRAIFLLNVPLAVAAAGLAVFFACESRDEKAKPLD
                     WKGAVAVAIGLAAITWGLGAIPASGFQDKAVLGALGAGIAFLILFVAIEARSRERAMM
                     PLSLYRSRNFSATNTVTLLLYFALGGVMYFLPFGLIRLGGYSATQAGAALLPFALIMG
                     FGASFAGAVSDRFGPRLSLTTGPIIAACGLALLAFADLGKSYWVGVFPAIGVLAIGVT
                     ITVPPLTSTVMASVGEAHAGVASGVNNAVARVAGLFAVAALGALLFASFSHHLAGTPP
                     AHANEALNAVLAGRADVTGGATAAFDRALRTVMLVTALCAALAGVAGWVWIRPIGSEA
                     TP"
     gene            4251..4544
                     /locus_tag="VN968_21755"
     CDS             4251..4544
                     /locus_tag="VN968_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31895.1"
                     /translation="MHQAANVQFERAVHEFGRWRAVPEDARSPAPAWWWGSAFEVLGV
                     PQPMPAAWCASLELPDSATYAAGAAIFLNALAGQTSLPWPGEFPRKAGHSGVG"
     gene            complement(4667..>6988)
                     /locus_tag="VN968_21760"
     CDS             complement(4667..>6988)
                     /locus_tag="VN968_21760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXL31896.1"
                     /translation="GEFLRIAASKGINYDRMRNRRFAVSATDPEGRGLTGTSFRTKKP
                     CIMNDFLADERTTHWHALAREDGTRSGASFPLLENGGRAVGVLLFLSREEGAFSDDLV
                     QLLGRLAENVSFALDNFDRAEEKAKTEEQKERLTRMFAALSATNEAIMRAKSRTQLFE
                     MVCEAAANGGKFTSTTISLANPDSDFLDIAAVAGPTAETTRNVKVSISAARPEGHGLS
                     GTAFRSGQARISNDYLADQQASAFHAIIRSDGAKSGAAFPLLIHGQPVGVMLFLSAEQ
                     DTFTTEFVELLQRLADNVSFALENFDRADEKTRADARIEYLASHDSLTSLPNRETFNG
                     LLHYAIEAARRHQRRFAVLFIDLDRFKVINDSLGHEAGDMLLVEIANRLRHTLRSSDV
                     VARLGGDEFVVILEEAAETHDVERITRDLLSVLSEPLQLSGHECHTTASIGIAMYPSD
                     GADVQTLTKNADMAMYLAKEDGKNGFRFFTKEIKTQSIERLTLESALRRALDRDQFAL
                     HYQPKVEMASGQITGVEALLRWTHPDLGVLPPAQFIPLAEETGLIVPIGRWVLREACA
                     QNMAWQRRGLRPVSMAVNLSPRQFLDEDLLQDIDEALAASGMPPVLLQLEVTESMVMR
                     NVSRAVKVLDAIQSRGIRLAIDDFGTGYSSMSLMKQFPIDTIKIDRSFVRDLPDDSED
                     KAITQAIISMGKALGMTIVAEGVETAEQETFLRDHACDEMQGFLFSKPVPSQQLAELL
                     RSAPLLISPPLQPAASATLENSTPRPERRSAAV"
BASE COUNT         1252 a         2146 c         2139 g         1451 t
ORIGIN      
        1 ctcagcatct ggccgggtcc gatatccaac accttggcat gatcgtcgta ccaggccttg
       61 tagggaagat cgacttcgac attgaagagc gtgatgtctt cgagatgctg cggcgtaatg
      121 aacggcgcgg tgttcggata gacatagacg cgcttgcgtc cggcgatctg gaccaggctc
      181 tgcgagggaa ggtcggcgtg gtaatagacc tctgcgcgcg gcgacgagat caggatgcct
      241 tcctggtggt ctttcggcac ctcgaagccg ggtaccttgg cagcgatttc ctcgaacatg
      301 cgatcgacca attggcgata gcggctatcg accgaaccga cttcgcgcag gttgagccaa
      361 agctcgccgc gggagatcgc gtcgatcacc tcgctccctc tgaggttgcc taagtcgccc
      421 tcgcgccata cgcggctgga gccccgggcg ccggtctgca caatgctgta atgttggcgc
      481 ggatagccct cgatcagctt ggcaagatcg tccatcgaga acgccggcga gttgtgcatc
      541 tcatgctcga ggcggatcgg ctggtgactc cacaattcgg agtgcgtctc gtcccatttg
      601 ctgaagatct ggctcgtcgt cattgtcgcg cccctcccgg cttcctgtct tccgttggct
      661 cggtaacgac catcccgggg cgcttctttt cagcgctgtc ccgaaaacat tctgccgtcg
      721 cctcgcggca tcgtcagctg tcccgatatg agacggctgg tcgaccttca cctgctattt
      781 ggcaagaagg gtgccgcacg gcaggcgaaa ccgctagccg gaaaataatg tcccggatgt
      841 atggctaacg cgagtgttct cgaagcggca cggcacccgc actgcacaat gacttcagac
      901 cccgcgcgcg ggcccggatt aaccttgacg cggtcgatcg gcaaacacat gggcttgcag
      961 ccccgcccca tgcaacggcg cgtccttgtc gtccaacgag gccgcagctg aggtaacgcg
     1021 ttgtgccggc gatggaccgc ccctcatcac gcacaaaaat gactggaacg aatccgccgg
     1081 cctccgcttg agttctcggt tttgttgcgc aaggagttcg gtttgaacac gcccgatcaa
     1141 gaaacggtat tgcgcgcgat ggaggatgca cgtcgcatcc tggcggaata tatcgcacct
     1201 ggccaacacg acgcgatgcg cactgtcgag cgcctgctca ccgtgctcga caagagcgat
     1261 gttgtgcatg ccctcgaccg aatgaaacga tgcagagttc agcggctggt cgattgaacg
     1321 gggacctccc gtatcgtcgc acgttgactg cgcagccgcg cggatctcag cgcggttagg
     1381 ctcgggcgtc gccccgccgt cggcgcggcg tccaggccgc cgcgatgtcc accttctcag
     1441 gccgcgtgtg tcccgatcgc aacagctttc ttgtttctcg catcgaacag gattttggac
     1501 tccacaccac cagcgagcgg cgtaatctga aaacaccggg gctgagggtg cggtttcagt
     1561 tccaaagtat tttgagcccg gcgggccgct ggtcatactc ccggtggccc cttttcatcg
     1621 tcgagcgggc cttcagcgct ctacagctcc ttgatctgca ccttgcggaa ttagaccacg
     1681 ccggagccgt gttggagcgc gaacgatccg cctaaatgtt tgctgtcctg cacgtcggcg
     1741 gttttttcac cgttcagcac gacaacaaga tgcggaccct gcgccgtgat ttcatagaca
     1801 ttccatttgc cggcgggttc ggcatcggat cgaccttcta cgcgtcttgc accgcgggaa
     1861 tgagaccggc tagcatgaac gcaatgaaaa gatgagctgg cggcagcgct tcaattcgca
     1921 caaatcaacg gaggaaacca tgaaggccac aaaacggttt tggcgatacc tcgcgatgcc
     1981 gctcgctctc gtcggcatgc tgagcctaag ctcggcggcc gaactcaatc ccgccgcggt
     2041 gatctacaaa ttaccggatc agattccgtg gagcccggtc gacgcccgcg gcgggcagaa
     2101 cgctgtcgtt gtcggcgatc cggccaagcc ggggttttat gccgtctaca ccaagtggac
     2161 caagggcaat catttcagcc ggccgcattt ccatcccaac gaccgttaca tcgccgtatt
     2221 gcaaggtacc tggtgggtgg gctccggccc aaaattcgac cccgccaaca cgaccgcaat
     2281 gccggcggga agtttcgtca cgcatttcgg caagcaggtg cattgggatg gcgccaagga
     2341 cgaggacgcc gtgcttttga tcatgggcga aggaccggcg acatcgaccg ccgcggagga
     2401 gaagtaggag ccagcaagca tgcgcggagc ggctgctgcc gcgttgccaa tggggcgcat
     2461 gaggcgcacc cttgcaactt gttagccacg ctctcccgag gccccgatga ttggtttcac
     2521 acggccgccc tgcgacgccg gaatcatccg cgcgacgtcg gctgctcccg cggccttgtc
     2581 gcgacggcgc aagcagatga cactggcagc gacgatcctc gggtcgagca tggcgtttat
     2641 cgacggctcg gtcgtgaata ttgccctgcc cggcatccag caggcgctgc aggcgaacgc
     2701 ggcttccacg cagtggatcg tgaacggcta cctgttgctg ctcggcgcgc tggtgctggt
     2761 cggaggctcg gctgccgatc tttacggccg caggcggatt ttcctggtcg gcattgcaat
     2821 atttaccggg gcctccattg cctgcggact ctcgcccgac atcacggtgc tcgttgtcag
     2881 ccgcgcggtt caaggactgg gcgccgcact gctgataccg gccagcctcg cgatgcttgg
     2941 tgccactttt gacgaccatg agcgcagcca tgcgatcggg acctgggcgg gtgtcggcgc
     3001 gctgacggcg gcggctggtc ccgtgctcgg cggctggctg gtcgaccagg tgtcgtggcg
     3061 tgcgatcttc ctgctgaatg ttccgctggc ggttgccgcg gccggacttg cggtcttctt
     3121 tgcctgcgaa agccgcgatg aaaaggccaa gccgctcgac tggaaaggcg cggtcgcggt
     3181 cgccatcggc cttgccgcaa tcacctgggg attgggcgcc attccggcgt cgggatttca
     3241 ggacaaagcc gtgctcggcg cgctgggcgc gggaatagcg ttcctgatat tgttcgtcgc
     3301 gatcgaggcc cgctcccgcg aacgggcgat gatgccgctg tcgctgtacc gctcacgcaa
     3361 tttttccgca accaacaccg tgacgctgct gctctacttc gccctcggcg gcgtgatgta
     3421 ttttctcccc ttcggcctga tccggctcgg cggttattcc gcgacgcagg ccggcgcggc
     3481 gctgctgccg ttcgcattga tcatggggtt cggagcatcg ttcgcgggtg ccgtctccga
     3541 ccgtttcggg ccgcggctgt cgctgaccac cgggcccatc atcgctgcct gcggattggc
     3601 gctgctcgcc tttgccgacc tcggaaaatc ctattgggtc ggcgtgtttc cagcgatcgg
     3661 cgtgcttgcg atcggcgtga ccatcaccgt gccgccgttg acatcgacgg tgatggcatc
     3721 ggtgggcgaa gctcacgccg gcgttgcttc cggcgtcaac aatgccgttg cacgcgtggc
     3781 cggcctgttt gccgtcgcgg cgctcggcgc gctgctgttc gcgagctttt cgcatcacct
     3841 tgccggaacg ccacccgcgc acgccaacga agcattgaat gcggttctgg ccggccgggc
     3901 cgatgtcacc ggcggcgcaa ccgccgcatt cgatcgcgcg ctgcgcacgg tgatgctggt
     3961 gaccgcactc tgcgccgcgc ttgccggcgt cgcgggctgg gtgtggatcc ggccgatcgg
     4021 ttcggaagcg acaccctgag acgcaacttt tcaagaaatc gcgcgtcgca cattttcaat
     4081 gatttcaggg agatgagata tggccgatca tgtcgaccag cacgtgcatc tgaacaagtc
     4141 tggcgcggat gtgacgtcgc actggcagca cggacaacag taaacgcatc ggcgcgcgtt
     4201 gcgcccgttg cagataggcg tatgatctcg gaggatcagg gggaacttca atgcatcagg
     4261 ccgcgaatgt gcaatttgaa cgcgccgtgc acgaattcgg ccgatggcgc gctgtccccg
     4321 aagacgcgag atcgccggct ccggcatggt ggtgggggtc ggcgttcgag gtacttggcg
     4381 tgccgcaacc gatgccggcg gcgtggtgtg caagtctgga attgccggat agcgccacct
     4441 acgccgcggg cgcggcaatt tttctcaatg cgctcgccgg ccagacgtcg ctgccctggc
     4501 ccggcgaatt tccgcgcaag gcaggacatt cgggtgttgg ctagagcggg atgactttat
     4561 cttcgaatcg tcatccgctc tatctttttg tttgagcatg atctccgcgc aaacgcttcg
     4621 cgtttgtcgc gagggaaaac cggtccccac ttttccggat catgctctag acggctgcac
     4681 ttctccgttc tggccgcgga gttgagttct cgagtgtggc gcttgctgca ggctgaagag
     4741 gcggagatat cagcagaggc gcggagcgaa gcagttctgc caactgctgc gacggcactg
     4801 gtttgctgaa cagaaaaccc tgcatttcat cgcaagcgtg atcacgcaag aaagtctcct
     4861 gttcggcggt ttccacacct tcggcaacga tcgtcatccc gagcgccttg cccatgctga
     4921 tgatcgcctg ggtgatggcc ttgtcctcag agtcatcggg aagatcgcga acaaaggaac
     4981 ggtcgatctt gatggtgtcg atcggaaatt gcttcatcag ggacatcgac gaatagccgg
     5041 ttccgaaatc gtcgatcgca agacgaatgc cgcggctttg gatggcgtcc agcactttga
     5101 ccgcccggga gacgtttcgc atcaccatgc tttcagtgac ctcgagctgc agcagcaccg
     5161 gcggcatgcc gctcgccgcc agcgcctcgt cgatatcctg caacaagtcc tcatcaagga
     5221 actgcctcgg cgagagattg accgccatcg ataccggccg caacccgcgg cgctgccagg
     5281 ccatgttttg cgcgcaggct tccctcaaga cccagcggcc gatcgggacg atcaagccgg
     5341 tttcctcggc aagcggtatg aactgtgcgg gcggcaacac gccgagatcg ggatgggtcc
     5401 agcgcaacag cgcctcgacg ccggttattt gtccgctcgc catctccacc ttcggctgat
     5461 aatgcagcgc gaactgatcg cgatcgaggg cacgacgcag cgccgattcc agcgtcaggc
     5521 gctcaatcga ctgggtcttg atctccttgg tgaagaagcg gaagccgttc ttgccatctt
     5581 ccttggcgag gtacatcgcc atgtcggcgt tcttcgtcag cgtctgaaca tcggcgccat
     5641 ccgaggggta catggcgatg ccgatgctgg ccgtggtatg acattcgtgg ccgctcaatt
     5701 gcaaaggttc gctgaggacc gacaacaagt cgcgggtaat gcgttcgacg tcatgagttt
     5761 ccgcggcttc ctccagtatg acgacgaatt catcgccgcc cagcctcgcc accacgtcgc
     5821 ttgaacgcaa ggtatgtcgc agccggttgg cgatttccac cagcaacata tcgcccgctt
     5881 catggcccag cgagtcgttg atgaccttga acctgtcgag atcgatgaac aataccgcaa
     5941 accgccgctg gtggcgacgc gcggcttcga tcgcgtagtg aagcaatccg ttgaacgttt
     6001 cccgattggg cagactggtc aggctgtcat gcgacgcgag gtattcgatc cgcgcgtccg
     6061 ccctggtttt ttcgtccgcg cggtcgaagt tctcaagggc aaacgacaca ttgtcagcca
     6121 gccgttgcag gagttcgacg aattcggttg taaaggtgtc ttgttcggcg gaaagaaaca
     6181 gcatgacgcc aacgggctgc ccatggatca gcagcggaaa tgccgcgccc gactttgccc
     6241 catcgctgcg aatgatggcg tggaaagcac tggcttgctg gtcagccaga taatcgttgc
     6301 tgatgcgagc ctgcccggaa cggaacgcgg tgccgctaag cccgtgcccc tccgggcgcg
     6361 cggcgctgat cgaaaccttt acgtttcgtg ttgtctccgc ggtcggcccc gccactgccg
     6421 cgatgtcgag gaaatcgctg tcgggattgg ccagactgat ggtagtcgag gtgaatttgc
     6481 cgccattcgc cgctgcctcg cacaccattt cgaacagttg cgtccgcgat ttggctcgca
     6541 tgatcgcttc gttggttgcg ctaagcgcgg cgaacattcg cgtcagccgc tccttctgct
     6601 cctcggtctt tgccttttcc tctgcgcggt cgaagttatc gagggcgaat gaaacgtttt
     6661 ctgccagcct tccaaggagc tgcaccagat catccgaaaa agcgccttct tcacgggaaa
     6721 gaaacagcag cacaccgacg gctctgccgc cattttccag caagggaaag cttgctccgg
     6781 atcgtgtccc gtcttccctt gcaagggcat gccaatgagt ggttcgctca tcggccagga
     6841 aatcgttcat gatgcagggt ttttttgtcc gaaacgacgt tccggtcaaa ccccggcctt
     6901 cggggtccgt agccgagacc gcaaagcggc ggtttctcat gcggtcgtaa tttatccctt
     6961 tcgacgcagc aattcgaagg aactcccc
//
LOCUS       DATNOI010000536         3521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2036156,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000536 DATNOI010000000
VERSION     DATNOI010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2036156"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VN968_21765"
     CDS             <1..195
                     /locus_tag="VN968_21765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HXL31897.1"
                     /translation="RFWFVALIDFAIHITVDRAKGYCSSRFCVTQENHHPWFWTLIGV
                     DQALHHLTGFGLSIFMAANG"
     gene            391..1272
                     /locus_tag="VN968_21770"
     CDS             391..1272
                     /locus_tag="VN968_21770"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HXL31898.1"
                     /translation="MTEQPLAKTTVDVLGSRMAYHERGEGTPVLFLHGNPTSSYLWRD
                     VIPELEGCGRLIAPDLIGMGDSAKLPNPDADTYRFTTHRKYLAGFIDAVIGPAQPIVF
                     VVHDWGSALGFDWANHHRDRVRGIAYMEGIVRPVAGWEEWSAVATPIFQGFRSDKGEA
                     MILDRNMFVERVLPGSVLRKLTEAEMTEYRRPFLQARDRWPTLTWPRQIPLSGEPADV
                     VQIATDYSRWMAENDIPKLFVNADPGAILIGAVRDFCRSWKSQTEVTVPGSHFIQEDS
                     GPAIGRAVAEWMKANSL"
     gene            1395..1799
                     /locus_tag="VN968_21775"
     CDS             1395..1799
                     /locus_tag="VN968_21775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31899.1"
                     /translation="MFSSRDAYENDFCPVRDELLGEMYRANANGLAQLVESVSSDVRA
                     MLALFCYRRSHLHALALSIAATCSERELIHVGGRVGSTLYALSRETPATRSAPSSHGG
                     RKPITLSTRPLSTFSPVEDEFDGEDLLQAATA"
     gene            complement(1944..2264)
                     /locus_tag="VN968_21780"
     CDS             complement(1944..2264)
                     /locus_tag="VN968_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HXL31900.1"
                     /translation="METSSPAIYVQSPCQACGACCSYSSNWPRFTTEDDAALDLIPEK
                     FVNEKLSGMRCDGERCSALSGKVGVATSCVIYAVRPEVCRTCMPGDAECAMARRRHGL
                     PVLT"
     gene            complement(2346..3044)
                     /locus_tag="VN968_21785"
     CDS             complement(2346..3044)
                     /locus_tag="VN968_21785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HXL31901.1"
                     /translation="MRRADRLFQIIQVLRRTRKPLTADAIAAELETSKRTIYRDIATL
                     IAQRVPIRGEAGMGYIMEKGFDLPPLMLTPDEIEAAVLGAQWVAGHADSVLARAAEDL
                     MAKIADTVPERLRPFVLEPASRARPGWRREPDRIDMARTRAQIHEGKKITLHYRDEHG
                     RDSERTIWPIAIGYLEAVRLLAAWCELRRDFRSFRTDRVIDAVHLDEKYPERRDLLRA
                     RWRRSLVWEPPSDT"
     gene            3147..3419
                     /locus_tag="VN968_21790"
     CDS             3147..3419
                     /locus_tag="VN968_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31902.1"
                     /translation="MMILYGFGGFQPFWRAQVWAVEHLISVHCASGDFRWAIAALALR
                     WLAALEAFAQKQLVCLARTVMGRAWRGARFAADITDADTMVATGGS"
BASE COUNT          622 a         1071 c         1108 g          720 t
ORIGIN      
        1 cgattctggt ttgttgccct gatcgacttt gcgattcaca tcacggtcga ccgcgccaag
       61 ggatattgct catcgaggtt ttgcgtgacg caggagaatc atcatccatg gttctggacg
      121 ctgatcgggg tagaccaggc attgcatcac cttaccggat tcggcctgtc gattttcatg
      181 gcggcgaacg gttgatattg ctggcttccg cgcctgtgcc gtcatgggcg gacagcgcaa
      241 acggaatcga cgcaccatcc gggagcgatg tcttgcttca cccagcgttt aacgggaagc
      301 gagtcaaatc ttcaggcgca ctgccgcaat ttgcgttaga ttcaatcgcg ccgcatgctc
      361 gcgaagcgtg ctgattgatc gagagctccg atgacagaac aaccgctcgc aaaaacgacc
      421 gtcgatgtgc tgggtagccg catggcctat cacgagcgcg gcgagggaac gccggtgttg
      481 tttctgcatg gcaacccgac ctcgtcctat ctttggcggg acgtcattcc cgaactggaa
      541 ggatgcggcc gcctgatcgc gccggatctg atcggcatgg gcgattccgc caaactgcca
      601 aatcccgatg ccgacaccta ccgcttcacg acccaccgca aatatctggc gggcttcatc
      661 gatgcggtca tcggtcccgc gcaacccatc gtgttcgtgg tgcacgactg gggctcggcg
      721 ctcggtttcg actgggccaa ccatcaccgc gatcgcgtcc gcggcatcgc ctatatggag
      781 gggatcgtgc ggccggtcgc cggttgggag gaatggagcg cggtggcgac gccgatcttc
      841 caggggtttc gctccgacaa gggcgaagca atgatcctcg accgcaacat gttcgtcgag
      901 cgggtgctgc ccggctcggt cttgaggaaa ctgaccgagg ccgagatgac ggagtatcgc
      961 cggccgtttc tgcaagccag ggaccgctgg ccgacgctga cctggccgcg gcagattccg
     1021 ctatcgggcg aacccgccga tgtagtccag atcgcgacgg attattcgag gtggatggcc
     1081 gagaacgaca ttccgaaact gtttgttaac gctgatcccg gcgcgattct gatcggtgcc
     1141 gtccgggatt tttgccgaag ctggaaaagc cagaccgaag tcaccgtgcc aggctcgcat
     1201 ttcatccagg aagattccgg gccggcgatc ggccgggcgg tggccgagtg gatgaaggca
     1261 aactcgttat agggggactg gggctggcgc cgtcccgatt cgccgcggtc aagatacccg
     1321 tgtcactacc gggtagggtg gttaaccttt cgttagaggg ttacgctgca tcttgctgac
     1381 aggggagacc gcaaatgttc tcaagccgcg atgcctacga aaacgatttc tgtccggtcc
     1441 gggacgagtt gctcggtgaa atgtaccgcg ccaacgcgaa cgggttggcg cagctggtgg
     1501 agagcgtctc ttccgatgtt cgggcgatgc tggcgctatt ctgctatcgc agaagccacc
     1561 tgcacgcgct ggcactctcg atcgccgcaa cctgcagcga acgcgaattg atccacgtgg
     1621 gcggacgcgt cggctccacg ctttacgcgc tgtcccgcga aacgccggcg acgcgttccg
     1681 cgccgtcgtc ccacggcggc cgcaagccga tcacgctctc gaccagaccg ctcagcacct
     1741 tctcgccggt tgaagacgag ttcgacggcg aagatttact gcaagccgcg acggcgtgat
     1801 cgatcacgta atttctccgc gcgtcatccc ggcgaacgcc gggatccaca accaccaaca
     1861 tcaattgttg cggaaggcat ttgcctcggt gcctgaacga tagtgcacgg agcatagatc
     1921 ccggccttcg ccgggacgac tcgtcaggtc agcaccggca ggccgtgccg tctccgcgcc
     1981 atggcgcatt cggcatcgcc gggcatgcag gtccggcaga cttcgggccg cacggcataa
     2041 atcacgcacg acgtcgccac gccgaccttg cctgacagcg ccgagcatcg ctcgccgtcg
     2101 cagcgcattc ccgacagttt ttcattgacg aatttttccg gaatcagatc gagcgccgcg
     2161 tcgtcctcgg tggtgaagcg cggccagttc gacgaatacg aacagcaggc gccgcaggcc
     2221 tggcacgggc tttggacgta aatagctgga ctgcttgtct ccatgccttc cgtttagagc
     2281 atgattcgga aaactggcaa ccggctttcc tcgggacaaa ccggccggtt ggctgcacca
     2341 gcgcgtcaag tgtcactcgg gggttcccag accaggctcc ttcgccatct cgctctgagc
     2401 aagtcgcgcc gctcggggta cttttcgtcc agatgcacgg cgtcgatgac gcggtcggtt
     2461 cgaaagctgc gaaaatccct gcgcaactcg caccacgcgg ccagcagccg cacggcttcg
     2521 agatagccga tggcgatcgg ccagatggtg cgttcgctgt cgcggccgtg ttcgtcgcgg
     2581 tagtgcagcg tgatcttctt tccttcgtga atctgcgccc gcgtccgcgc catgtcgatg
     2641 cggtcgggtt ccctgcgcca gcccgggcgg gcgcggctgg cgggttcgag cacaaaaggc
     2701 ctcaggcgtt ccgggaccgt gtcggcgatc ttggccatca ggtcttcggc ggctcttgcc
     2761 agcacggaat cggcgtgtcc tgccacccat tgcgcgccga gcacggccgc ttcgatctcg
     2821 tcgggggtga gcatcagcgg cggcaggtca aaccccttct ccataatata acccatgccg
     2881 gcctcgccgc ggatcggtac ccgttgcgcg atcagggtgg cgatgtcgcg gtagatggtt
     2941 cgcttcgagg tctctaactc ggctgcgatc gcatccgcgg tcagtggttt ccgggtgcgg
     3001 cggagcacct gaatgatctg aaacagccgg tcggcgcgtc tcatggggcg tctcttttta
     3061 cagggcagcc gcgccgatgc tgacagcatg ttggcagcag gcggtggcta taccgggaca
     3121 acacttcaac tgaagggaac ttgtccatga tgattcttta tggcttcgga ggtttccagc
     3181 cgttctggcg ggcccaggtt tgggccgtgg agcacctgat ttccgtacac tgcgcctccg
     3241 gggatttccg ctgggcgatc gccgcgctgg ccttgcgttg gttggccgcg ttggaggctt
     3301 tcgcgcagaa gcaactggtc tgtttggccc gaaccgtcat gggtcgcgcc tggcgggggg
     3361 ctcgtttcgc ggccgatata accgatgctg acactatggt ggcaacaggg ggtagctagg
     3421 gtcgcgcatc atttcaacag atcggagccc tcacatgatc attctctatg gctttggccc
     3481 cggcttcggc ttgccggaaa tcagcccgtt tgtgaccaag a
//
LOCUS       DATNOI010000537        10802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_743254,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000537 DATNOI010000000
VERSION     DATNOI010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10802
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743254"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VN968_21795"
     CDS             <1..485
                     /locus_tag="VN968_21795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HXL31903.1"
                     /translation="IVHGHVRDEDGRGVPNTLLEIWQANACGRYIHVVDQHPAPLDPN
                     FTVAGRARSDSEGYYRFVTVKPGAYPWGNHHNAWRPAHIHFSVFGHAFISRLVTQMYF
                     PGDPLFPFDPIFNSVPDEKARLRMVSSFDLENTQPEWALCYRFDIVLRGRNATPMETK
                     "
     gene            485..1090
                     /gene="pcaG"
                     /locus_tag="VN968_21800"
     CDS             485..1090
                     /gene="pcaG"
                     /locus_tag="VN968_21800"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HXL31904.1"
                     /translation="MPGHKEQGITPSQTVGPYFKYGLTPNGQYQWNDAFTNNLVTPDV
                     SGDRIRVEGRVFDGDGQPVSDCMLEIWQADTQGRFADPQDQRALPNSAFRGFGRCGTD
                     ANGDYAFDTIKPGPLPDPDGKPQAPHILLAVFARGMLLHLYTRIYLGGEAANTADPVL
                     ALVPAERRDTLIAAREPGNGNAVYRFDIRLQGDNETVFFDV"
     gene            1280..2338
                     /locus_tag="VN968_21805"
     CDS             1280..2338
                     /locus_tag="VN968_21805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HXL31905.1"
                     /translation="MITQSLPPAASADHLTTALRNSGMLGDGCVRDVVVERSFDTLLS
                     HIFRLKLSYDATLGGPEFLILKAGLADRPGGPWKAGRQEVAFYTEVASAMSDHLVPRC
                     FDADWTADTGAWHLLLEDLTASHAVATRWPLPPTLAQCESIVRARARFHAAWWDDPRL
                     GNTIGSWQDAALDQQLLDFARDLEGFTERFGDRLSFERRDLYARLIDRAPRLQMRYRS
                     HRNMTILQGDSHVWNCFLPRAGASDDALLFDWDAWHIGPASNDLSYMMALHWYPDRRR
                     QVERPLLDCHHAALEAHGVKGYARRELDDDYRQSVLWNLRTPVWQEANGIPPVVWWNG
                     LERILMAVDDLDCRELLG"
     gene            complement(2414..2998)
                     /locus_tag="VN968_21810"
     CDS             complement(2414..2998)
                     /locus_tag="VN968_21810"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXL31906.1"
                     /translation="MLQARGRASRNVVDRIVELVAERPALAAALTGRLLDVGTGVGAV
                     ALEAAARSPSLAVVGLDVWEPALALARVNVAASPDAARIEIRAQDVTRLVETAAYTLA
                     WLPAPFLSQEVARAALDRLAAALARGGYLVVGLYLPPADPTGAALAALRLIRSGGHVW
                     ESAAMVAELRSRGFIAVETCAGPPGVTFVLGRLA"
     gene            complement(3088..4647)
                     /locus_tag="VN968_21815"
     CDS             complement(3088..4647)
                     /locus_tag="VN968_21815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HXL31907.1"
                     /translation="MPTAAASPIAVPGLRRNMVTICAMTATIMQALDTTIANVALPYM
                     QGTLSASQDQINWVLTSYIVAAAIMTAPVGWIANRFGRKRIFILCSGGFTIASVLCGL
                     AQDINQMVLFRLLQGVFGAALVPLSQAVMLDSYALHERAKAMSIWGMGVMMGPIMGPS
                     LGAWLTETYSWHWVFFVNLPFGVFTVLGLLVFMDETKTDRDLRFDWFGFAALAVGIGS
                     MQVALDRGEQLGWMESNEIIAEIIVSIAGFYYFFAHSLTTTKPFIQFAIFKDRNFVSG
                     CVFMAVMGLVLFSTMALSSPYLQNVIGYPIITAGLLLASRGCGTFVAMMLVGRMMRYI
                     EARTLIISGLGLTCLSLFYMTGWTDQTGVPEIVTLSIIQGFGFGLVFVPLSTVAFLTL
                     PNHLRTDGTSMLTLMRNVASSIGISVVIAQLTEGSRRVYAVLSQHINPFNNAMQMPNV
                     RGMIDMSTDAGRAMADVMVSLQAQIIAFSQDYQMVMLFTLCAIPLALMIGSSKAALRK
                     QAQAPDHAVIE"
     gene            complement(4654..5814)
                     /locus_tag="VN968_21820"
     CDS             complement(4654..5814)
                     /locus_tag="VN968_21820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HXL31908.1"
                     /translation="MADPVLKFPPDQKGNPPAAVPGVEAKPPRRAMAFVRRFRRTFLL
                     VVLPLLAAIGGLAFYLNGGRYVTTDDAYVGAQKVLITPDISGKIEKVVVREGQQVNEG
                     DVLFEIDPVPFRLAMQQAKANLDQTHTTYDNLKANVRIYGDMLDLAQQGVDLKRRDVD
                     RKASLVKSNVGSQLDLDNAGTALVTASAQLEFLKQQLSSSKTQLLGNPDLPIEQFPPY
                     AQAKASLDQAERNLDHTVMRAPMNGIATQVDQIQLGRFVMAGTPVFSVIDTSKPWVDA
                     NPKESDFTYVTVGQPVSIDVDAFPDHVFKGTIGSLSPGTGAQFAILPPQNATGNFVKV
                     VQRVPVRIYFDSNDKYVQKLKAGMSAYTTIDTGHRRSLAALLGFAPAAANQD"
     gene            complement(5807..6289)
                     /locus_tag="VN968_21825"
     CDS             complement(5807..6289)
                     /locus_tag="VN968_21825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXL31909.1"
                     /translation="MSRGSVDMNFLFTLGELQRLVRAYADKQAARYGITRAQWAVLAK
                     VERTEGLKQTELAEQMEMQPITLTRLIDRLCDNGWIERRSDDTDRRVNRLYLLKAARP
                     LLGKLSGLRSELTATALEGISPADAHRMLTQLEAIKENVRNAIQNPAGEPPRKEQRVG
                     "
     gene            6459..6953
                     /locus_tag="VN968_21830"
     CDS             6459..6953
                     /locus_tag="VN968_21830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HXL31910.1"
                     /translation="MALTKPTFPAPDHDHDRCAADGIDHAERVCVQRAQKFTPIRRQV
                     LQALLSSHRPLGAYEVIDQLAKSMPRPAPITVYRALDFLMENGLVHRIESRNAYLACA
                     HDHDAAAMVAFLICERCGSVGEIPAAPVAQSLNTAARATGFAPKLSVVEITGTCAHCQ
                     KTTL"
     gene            7043..7993
                     /locus_tag="VN968_21835"
     CDS             7043..7993
                     /locus_tag="VN968_21835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXL31911.1"
                     /translation="MSSEQSIVPSNGRPLSPGAVALMLMLCLSWGFNQIAVKLALPDI
                     PPMLQAMIRSAGALPVLLLIAKARGVKIFASDGTLVAGLFAGLLFGLEFVLIFRGLLL
                     TSASRAVVFLYTAPFFVALGSYQFLGERLRASQWGGLGLSFAGVALAIGVPQANVDAN
                     VLLGDLMIVAGGALWAATTLLVKATALLQAAPEKALGYQVALSIPILGVAAWISGETI
                     TRLPGPLALSLMAYQAIWVVGLTFLLWFTLVKSYSASKLSAFTFITPLFGVVASYFIM
                     HDTLTPVFGAAALLVIAGLYLVNRPNASGPKVVPDPNVPA"
     gene            complement(8005..8880)
                     /locus_tag="VN968_21840"
     CDS             complement(8005..8880)
                     /locus_tag="VN968_21840"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HXL31912.1"
                     /translation="MSERDQLMTTAELAATLDQPELRLFDCTTYLEYQPAGSDIPYIA
                     VPGRRTFEAGHIPGADFLDLQGEFSDQNTTLHFMMPATAQLEAAFGRHGIGANSQVVL
                     YSIGSAMWATRFWWMLSSLGYENVSVLDGGLDKWKAEGRAIKTGPAEGYPPASFTAKT
                     QRGYFVDKHEALAASTDRNTFVVNALSPQLHRGLEPSRYGRPGRIPGSVNVSAATLFD
                     PETKTFVPLADAAAKFASQGIAKDKRVVAYCGGGISATIDLFLLHRLGHDNLGLYDGS
                     MGEWAKDASLPIETG"
     gene            9161..10453
                     /gene="ispG"
                     /locus_tag="VN968_21845"
                     /gene_synonym="gcpE"
     CDS             9161..10453
                     /gene="ispG"
                     /locus_tag="VN968_21845"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HXL31913.1"
                     /translation="MNQPVKPPQDDVSSPSARRKTTQVMVGNVAAGGGAPIVVQSMTN
                     TDTADVAGTVAQIAALSRAGSEMVRITVDRDEAAAAVPHIRDELRKRGITTPLIGDFH
                     YIGHKLLADYPACAEALDKYRINPGNVGFKNKRDSQFTDIVEIALKNNKAVRIGANWG
                     SLDQELLTKLMDENALLAEPKDVRAVTREAMVQSALLSAARAEEIGLPKNKMILSAKV
                     SAVQDLIAVYQTLASRSDYAIHLGLTEAGMGSKGIVASAAALGILLQDGIGDTIRISL
                     TPEPGGDRTLEVQVAQELLQTMGFRTFVPLVAACPGCGRTTSTTFQELARSIQDFIRV
                     EMPNWKTRYPGVESLNVAIMGCIVNGPGESKHADIGISLPGTGETPAAPVFVDGKKFR
                     TLRGPNIATEFKAMVIDYIDQRYGAGANVPASTVTAAE"
     gene            10476..>10802
                     /locus_tag="VN968_21850"
     CDS             10476..>10802
                     /locus_tag="VN968_21850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31914.1"
                     /translation="MIETSLTTPSPAGRSGLFRQSHFRTSNSKMPRTPFSIAPTMRRK
                     LSSVLVATGIFACLGAVTTAAVGRPVEIGTMNAVLIGTGIGLFEEFYVQSPRGNWIRA
                     MHPLRAI"
BASE COUNT         1958 a         3389 c         3440 g         2015 t
ORIGIN      
        1 tcatcgtgca cggccatgta agggacgaag acggccgcgg cgtgccgaat acgctgctcg
       61 agatctggca ggccaacgcc tgcggccgct acatccatgt ggtcgatcag catccagcac
      121 cgctcgatcc gaatttcacc gtcgccggcc gcgcgcggtc ggatagcgaa ggctactaca
      181 ggttcgtcac ggtcaaaccc ggcgcctatc cgtgggggaa ccatcacaac gcctggcggc
      241 cggcccacat ccacttctcg gtgttcggcc acgccttcat ctcgcggctg gtgacgcaga
      301 tgtattttcc cggcgatccg ctatttccgt tcgatccgat cttcaattcg gtgccggacg
      361 agaaggcgcg gctgcgaatg gtttcctcct tcgatctgga aaacacccag ccggaatggg
      421 cgctgtgcta ccgcttcgac attgtgctgc gcgggcgcaa cgccacgccg atggagacaa
      481 aatagtgccg ggacataaag agcaggggat tacaccttcg cagaccgtcg gcccgtattt
      541 caagtatgga ctgacgccga acggccaata ccaatggaat gacgcattta ccaacaacct
      601 ggtcacgccg gatgtgtcag gcgatcgcat cagggtggag ggccgggtgt tcgatggcga
      661 cggccagccg gtgtcggatt gcatgctgga gatctggcag gcggatacgc aaggcaggtt
      721 tgccgatccg caggatcagc gcgcgctgcc caattcggca ttccggggct ttggccgctg
      781 cggtaccgac gccaacggcg attatgcgtt cgacaccatc aagcccggcc cgctgcccga
      841 tcccgacggc aagccgcagg cgccgcatat cctgctcgcg gtgtttgccc gcggcatgct
      901 gctgcatctc tatacgcgga tctatctggg tggcgaggcg gccaataccg ccgacccggt
      961 gctggcgctg gtgccggcgg agcggcgcga taccctgatc gccgcccgcg agccgggcaa
     1021 cggcaacgcg gtctatcgct tcgacatccg cctgcagggc gacaacgaga cggtgttttt
     1081 cgacgtgtga ggttttcggg aagctgcgtg cagcgttgcg ccatttttcc cccgtcatgg
     1141 ccgggcttga ccaggcttga cccggccatc catctgtttc gaggaatgct tcttgggaag
     1201 atgatggata cgcgggtcaa gcccgcgtaa gacgggagtt tgatgtggcg cgttgtgccc
     1261 cccgcacgag ggaccgtcaa tgataacgca atcgttgccg ccagcggcaa gtgccgacca
     1321 tctgacgacg gcgctgcgca attctggcat gcttggcgat ggctgcgtcc gcgatgtggt
     1381 ggtggagcgt tcgttcgata cgctgttgtc gcatatcttc cggctcaagc tcagttatga
     1441 cgccacgctc ggcgggccgg aattcctgat tctcaaggcg gggctggccg atcgccccgg
     1501 cggtccctgg aaggcgggac gccaggaggt cgcgttctac accgaagtcg cttccgccat
     1561 gtcggatcat ctggtcccgc gctgcttcga cgccgactgg accgccgaca ccggcgcatg
     1621 gcatctacta ctggaagatc tgactgcttc gcatgccgtg gctacgcgct ggccgttgcc
     1681 accgacgctt gcgcaatgcg agagcatcgt gcgggcgcgg gcgcgttttc atgccgcgtg
     1741 gtgggacgat ccgcgtctcg gcaatacaat cggttcatgg caggatgccg cgctggatca
     1801 gcaacttctg gattttgcca gagatctgga aggtttcacc gagcgctttg gcgatcgtct
     1861 ttcgttcgag cggcgcgatc tttacgcgcg gctcattgat cgcgcgcccc ggctgcagat
     1921 gcggtaccgg tcccatcgca acatgacgat ccttcaaggc gattcgcacg tctggaattg
     1981 ttttctgccg cgcgccggcg ccagcgatga tgccctgctg ttcgactggg atgcatggca
     2041 catcggcccc gcctccaacg atctctccta tatgatggcg ctgcactggt atccggaccg
     2101 ccgacggcag gtcgaacggc cgctgctgga ttgccaccat gcagcgttgg aggcccacgg
     2161 tgtgaaaggc tacgcgcgcc gcgaactgga tgacgactat cgccagtcgg tgctgtggaa
     2221 cctgaggacg ccggtgtggc aggaggccaa cggcatcccg ccggtggtct ggtggaatgg
     2281 tctggagcgg attttgatgg cggtcgacga tctcgactgc cgcgagctgc tgggttgacg
     2341 ggcgagagcg acaacaagcc agtgtttttc gatgtgagag tttttcgtct ccgcgaagcg
     2401 aggcttgcct cactcaggca agccggccca gtacaaatgt caccccgggt ggtccggcgc
     2461 aggtttccac cgcgataaaa ccgcgggaac gcagctcggc caccatcgcc gcgctttccc
     2521 acacatggcc gccactgcgg atcaaccgca gggcggcaag cgccgccccg gttgggtcag
     2581 cgggcggcag gtagagaccg accaccagat atcctccgcg cgcgagggcc gccgcaagcc
     2641 ggtcgagcgc ggcccgcgcc acctcttgcg acaggaacgg cgccggcagc catgcgaggg
     2701 tgtaggcggc cgtctcgacg agacgtgtca cgtcctgggc gcggatctcg atgcgcgcgg
     2761 catcggggct cgccgccacg ttgacccgcg ccaacgcaag cgccggctcc cagacgtcga
     2821 gtccgacgac tgccagcgac ggggaccgcg ccgccgcctc cagcgcaacg gcgccgacgc
     2881 cggtgcccac gtccagaaga cgcccggtca acgccgctgc cagggccgga cgctccgcca
     2941 ccaactcgac aatgcggtcg acgacgttgc gcgaggcgcg gccccgggcc tgcagcatcg
     3001 cggggtcacg cacgacccag cccggcggcc gatccggcct ctcgaacagc tcgcgtgctg
     3061 atgtgaccag gttgagagcg cgaggcgcta ctcgatcacc gcatggtccg gcgcctgcgc
     3121 ctgcttgcgc aaggcggcct tgctcgagcc gatcatgagc gccagcggga tcgcgcacag
     3181 cgtgaacagc atcaccatct ggtagtcctg cgagaacgcg atgatctgcg cctgcaggga
     3241 taccatcacg tccgccatcg cgcggcctgc gtcggtggac atgtcgatca tgccgcgcac
     3301 attgggcatc tgcatcgcgt tgttgaacgg gttgatgtgc tgcgacagca ccgcgtaaac
     3361 ccgccgcgag ccttcggtca gttgcgcgat cacgacggat atgccgatcg agcttgcgac
     3421 attgcgcatc agggtcagca tcgaggtgcc gtcggtgcgc agatgattgg gcagcgtcag
     3481 gaacgccacc gtgctcagcg gcacgaacac cagcccgaac ccgaagccct ggatgatgct
     3541 caaggtcacg atctcgggaa cgccggtctg atcggtccag ccggtcatat agaacagcga
     3601 cagacacgtc aggccgagcc ccgagatgat cagcgtgcgc gcctcgatat agcgcatcat
     3661 ccgccccacc agcatcatcg cgacaaaggt gccgcagcca cggctcgcca gcagcaggcc
     3721 ggcggtgatg atgggatagc cgatcacgtt ctgcagataa ggcgacgaca gcgccatagt
     3781 cgagaacagc acgaggccca tcaccgccat gaacacgcag ccgctgacga aattcctgtc
     3841 cttgaagatc gcgaactgga tgaagggctt tgtggtggtc agcgaatgcg caaagaaata
     3901 atagaagccg gcgatcgaga cgatgatctc ggcgatgatt tcgttggatt ccatccagcc
     3961 gagctgttcg ccgcggtcga gcgcaacctg catcgaaccg atgccgaccg cgagcgccgc
     4021 aaagccgaac cagtcgaagc gcagatcgcg atcggtcttg gtctcgtcca tgaagaccag
     4081 gaggccgagc acggtgaaga cgccgaacgg caggttgacg aagaacaccc agtgccagga
     4141 ataggtttcg gtcagccagg cgccgagcga cgggcccatg atcgggccca tcatcacgcc
     4201 catgccccag atcgacatcg ccttggcgcg ctcatgcagc gcgtaggaat cgagcatgac
     4261 ggcctgcgac agcggcacca gcgccgctcc gaatacgccc tgcagcaggc gaaacagcac
     4321 catctggttg atgtcctgcg ccagcccgca cagcaccgaa gcgatcgtga acccgccgga
     4381 acacaggatg aaaatgcgct tgcggccgaa ccggttggcg atccatccga ccggcgcggt
     4441 catgatcgcg gcggccacga tgtaggaggt cagcacccag ttgatctggt cctgcgacgc
     4501 cgacagggtg ccctgcatat aaggcagcgc gacattggcg atggtggtgt cgagcgcctg
     4561 catgatggtc gcggtcatgg cgcagatcgt caccatgttc cggcgcaggc cgggaaccgc
     4621 gatcggggat gcagccgcag tcggcatgat cggtcagtcc tggttcgccg ccgccggcgc
     4681 gaagccgagc agggcggcga gcgaacggcg gtggccggtg tcgatcgtgg tataggcgct
     4741 cataccagcc ttcaacttct ggacgtattt gtcgttgctg tcgaaataga ttcgcaccgg
     4801 cacccgctgc accaccttga cgaagttgcc ggtggcgttc tgcggcggca ggatcgcgaa
     4861 ttgcgcgccg gtgcccgggc tgagcgaacc gatggtgccc ttgaagacat gatccgggaa
     4921 ggcatcgacg tcgatgctga cgggttgccc caccgtgaca taggtgaagt cggattcctt
     4981 ggggttggcg tcgacccagg gtttcgaggt gtcgatgaca gagaacacag gagtgccggc
     5041 catgacaaag cggccgagct ggatctgatc aacctgcgtc gcgatgccgt tcatcggcgc
     5101 gcgcatcacg gtgtgatcaa ggttgcgttc ggcctggtcg agcgaagcct tggcctgggc
     5161 gtatggcgga aactgctcga tcggcaaatc gggattgccg agcaactggg ttttcgagga
     5221 ggacagctgc tgcttgagga attcgagctg cgcgctcgcg gtgacaaggg ccgtccccgc
     5281 attgtcgagg tcgagctgcg agccgacatt gctcttgacc agggatgcct tgcgatcgac
     5341 atcgcgtcgc ttcaggtcga caccctgctg cgccagatcg agcatgtcgc cgtaaatcct
     5401 gacgttggct ttcaggttgt cataggtggt gtgggtctgg tcgagattgg ccttggcttg
     5461 ctgcatggcg agccggaacg gcaccggatc gatctcgaac agcacgtcgc cctcgttgac
     5521 ctgctggcct tcgcgcacca cgaccttttc aatcttgccg gaaatgtccg gcgtgatcag
     5581 aaccttctgc gcgccgacat aggcgtcgtc ggtggtcacg tagcggcccc cgttcagata
     5641 gaaggccagc ccgccgatcg ccgccagcag cggcagcacg accagcagga acgtcctgcg
     5701 gaaacgccgg acaaaggcca tcgcacggcg tggcggcttt gcttcgacgc cgggaacggc
     5761 cgccggcgga ttgcccttct gatcgggcgg gaacttcagg actggatcag ccaacgcgct
     5821 gctccttcct cggcggttca ccggccggat tttgaatggc gttgcgcacg ttttccttga
     5881 tggcttccag ttgagtgagc atgcggtggg cgtcggccgg gctgatgcct tcgagcgcgg
     5941 tcgccgtcag ttcggagcgc agcccgctca atttgcccag caaggggcgt gcggccttga
     6001 gtagatagag ccggttgacg cggcggtcgg tgtcgtcgct gcgccgttcg atccagccat
     6061 tgtcgcagag ccgatcgatc aggcgtgtca gcgtgatcgg ctgcatttcc atctgttcgg
     6121 cgagttccgt ctgtttcagg ccctcggtgc gttccacctt ggccagcacc gcccactggg
     6181 cgcgggtaat accgtagcgc gcggcctgct tgtcggcata ggcgcgcacc agccgctgca
     6241 attcgccgag cgtaaacagg aagttcatat ccacggaacc gcgggacatc ggggccatct
     6301 ccgtaagctt acgatatgat aagctagctt atgaatatgg cttcgcaagg tagcttggca
     6361 agccccgaga agcctgacct gcaggcagca catggcttga ttgcgcgaga ttgggcggga
     6421 ttgggcttta tctcgccgaa atgctacaga tcgcagtaat ggccctgaca aaaccgacgt
     6481 ttcccgcgcc cgaccatgat cacgaccgct gcgccgcgga cgggatcgat catgccgagc
     6541 gggtctgcgt gcagcgggcg cagaaattca cgccgatccg gcgccaggtg ctgcaggcgc
     6601 tgttgtccag tcaccgtccg ctcggcgcct acgaggtgat cgatcaactc gccaaatcga
     6661 tgccgcggcc ggcgccgatc acggtgtacc gcgcgctgga tttcctgatg gagaacggcc
     6721 tcgtccaccg cattgaaagc cgcaatgcgt atctcgcatg cgcccatgac catgacgccg
     6781 cggcgatggt ggcgtttctg atctgcgagc gctgcggctc ggtcggcgag attccggccg
     6841 cgccggtcgc gcagagcctc aacaccgcgg cgcgcgccac cggctttgcg ccgaaattgt
     6901 ccgtggtcga gatcaccggc acctgcgcgc actgccagaa aaccactcta taaatgggca
     6961 tgatctgttc cgaaaaccgg aagaccactt ttcgggatca tgccccataa caacacggcg
     7021 acaagccggg ccacgagggt tcatgtcttc agaacaatcg attgttccat cgaacgggcg
     7081 tccgctcagc ccgggcgccg tcgcgctgat gctgatgctt tgcctgagct ggggtttcaa
     7141 tcagatcgcg gtaaaactgg cgctgccgga catcccgccg atgctgcagg caatgatccg
     7201 ctccgcgggc gcgttgccgg tgctgttgtt gatcgcgaag gcgcgcggcg tgaaaatctt
     7261 cgcttcggac ggtacgcttg tcgccggcct gttcgcgggc ctgttgttcg gccttgaatt
     7321 cgtgctgatt tttcgtggac tgctgctgac gtcggcgtcg cgcgcggtgg tgtttctcta
     7381 taccgcaccg ttttttgtgg cgcttggctc ctatcagttt ctcggcgaac gcttgcgcgc
     7441 ctcgcaatgg ggtggattgg gtttgagttt cgccggcgtg gcgctggcga tcggcgttcc
     7501 ccaggccaat gtcgacgcca atgtgctgct gggcgatctc atgatcgtcg ccggcggcgc
     7561 gctatgggcg gcgaccacgc tgctggtgaa ggcgacggcg ctgcttcagg ccgcgccgga
     7621 aaaggcgctg ggctatcagg tggcgctgtc gatcccgatt ttgggcgttg cggcctggat
     7681 atcaggcgaa accatcaccc gccttcccgg cccgctggcg ctgtcgctga tggcgtatca
     7741 ggccatttgg gtggtcggat tgacgtttct cttgtggttc acgctggtca aaagctattc
     7801 cgccagcaag ttgtcggctt ttacctttat cactcctctg tttggcgtgg tggccagcta
     7861 tttcatcatg cacgacaccc tgacccccgt gttcggcgcc gcggccctat tggtgattgc
     7921 cgggctgtat ctggtgaaca ggcccaatgc gtccgggccg aaggtcgtgc ccgatcccaa
     7981 cgttccggct taatgcagcc cgtctcagcc ggtctcgatc ggcagcgacg catccttcgc
     8041 ccattctccc atcgagccgt cgtaaaggcc gagattgtcg tggccgagcc gatgcagcag
     8101 gaacaagtcg atggtggccg agatgccgcc gccgcaatag gcgaccacgc gcttgtcttt
     8161 ggctatgccc tggcttgcga acttcgccgc ggcgtccgcg agcggaacaa aggtcttggt
     8221 ctccggatcg aacagcgtag ccgccgatac gttgacgctg ccgggaatgc gcccgggccg
     8281 gccgtaacgg cttggctcca aacccctgtg gagttgaggg ctgagcgcat tgacgacgaa
     8341 ggtgttgcga tccgtgctcg ccgcaagcgc ctcgtgcttg tcgacgaaat atccccgttg
     8401 ggttttcgcg gtgaagctcg ccggcggata tccctccgcc ggcccggttt tgatggcgcg
     8461 gccctccgct ttccatttgt ccaatccgcc atccagcacg gaaacgtttt cataacccag
     8521 cgagctcagc atccaccaga atcgcgtcgc ccacatcgcg gagccgatgc tgtaaagcac
     8581 cacttggctg ttcgcgccga tgccatggcg gccaaacgcg gcctcaagct gcgcggtcgc
     8641 cggcatcatg aagtgaagcg tcgtgttctg gtcggagaat tcgccctgca gatccagaaa
     8701 atccgctccc ggaatatgcc cagcctcaaa ggtacgccgt ccgggtacgg cgatataggg
     8761 aatgtcgctg ccggccggct gatattcgag gtaggtggta caatcgaaga gcctgagttc
     8821 tggctgatcg agggtggcgg cgagttccgc ggtcgtcata agctggtcgc gctcggacat
     8881 gggctcgcct cccgggaatg tctgggccga tgctacggcg tgccgtcaac gatgaaaagt
     8941 cggccgccgc cactgccttg ccctttaatc gcgggattcc ggtccaatat accgtctggc
     9001 atagcgcggc cggcagcccc ttgctttgga ccgaaatggc tggactaagc tattgaatga
     9061 atggacgaaa aattccaaac cgtcccaaac cgtgatgatg cgcacagagc tgccgccggc
     9121 gccgtggtga atgtcaccaa aacctgatat ttgaggcccc atgaaccagc cagtaaaacc
     9181 accgcaagac gatgtttcca gccccagcgc ccggcgtaaa acaacccagg tcatggtcgg
     9241 caatgttgcc gccggcgggg gcgcgccgat cgtagtgcag tcgatgacca acaccgacac
     9301 cgccgatgtc gcgggcaccg tggcgcagat cgcagcactt tcgcgtgccg gttccgaaat
     9361 ggtgcggatc acggtcgacc gcgacgaggc cgcggccgcg gtgccgcaca tccgcgacga
     9421 acttcgcaag cgcggcatca ccacccccct gatcggtgat ttccattata tcggccacaa
     9481 gctgttggcc gactatccgg cctgcgccga ggcgctcgac aaataccgca tcaatcccgg
     9541 caatgtcggc ttcaagaaca agcgcgacag ccagttcacc gacatcgtcg agatcgcact
     9601 caagaacaac aaggcggttc ggatcggcgc gaactggggt tcgctcgatc aggaactgct
     9661 gaccaagctg atggacgaaa acgcgctgct cgccgagccg aaggatgtcc gcgcggtgac
     9721 gcgcgaggcg atggtgcagt cggcgctgtt gtcggccgcg cgcgccgagg agatcggcct
     9781 gccgaagaac aagatgatcc tgtcggcgaa agtctcggcg gtgcaggacc tgatcgcggt
     9841 ctatcaaacg ctcgcctcgc gttcggatta tgccatccat ctcggcctca ccgaggccgg
     9901 catgggctcg aagggcatcg tggcctcggc tgccgcactc ggcatcctgc tgcaggacgg
     9961 catcggcgac accatccgga tttcgctgac gcccgagccc ggcggcgatc gtacgctgga
    10021 agtgcaggtc gcgcaggaac tgctgcagac catgggtttt cgcacctttg tgccgctggt
    10081 cgcggcatgc cccggctgcg gccgcaccac ctcgaccacc ttccaggaac tggcgcgctc
    10141 gatccaggat ttcatccgcg ttgaaatgcc caactggaag acgcgctatc ccggcgtcga
    10201 atcgctcaac gtcgcgatca tgggctgcat cgtcaacggc ccgggcgagt ccaagcatgc
    10261 cgacatcggc atttccctgc ccggcactgg cgagacgccg gcggcgccgg tgtttgtcga
    10321 cggcaagaaa ttccgcacct tgcgcgggcc caacatcgcc accgaattca aggcgatggt
    10381 gatcgactat atcgaccagc gctacggcgc gggggcgaac gttcccgcaa gcacggtgac
    10441 cgcggcggag tagttcgtcc cgtagccgaa agaccatgat cgagaccagc ctgacgaccc
    10501 catcgccagc tggacgctcg ggcctgttcc gccagtccca ttttcgcaca agcaattcca
    10561 aaatgccgcg cacccctttc tcaatcgcac ctacgatgcg ccgcaagctt tcgtccgttc
    10621 tggtggcaac gggcatcttc gcgtgcctgg gtgcggtcac tacggcagcc gtcgggcggc
    10681 cggtcgagat cggcacgatg aacgcggttc tgatcggaac cggaattggg ttgttcgagg
    10741 aattctatgt ccagagtccg aggggcaact ggatacgcgc catgcacccg ctacgcgcca
    10801 tt
//
LOCUS       DATNOI010000538         8261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2582538,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000538 DATNOI010000000
VERSION     DATNOI010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8261
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2582538"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3457)
                     /locus_tag="VN968_21855"
     CDS             complement(<1..3457)
                     /locus_tag="VN968_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL31915.1"
                     /translation="MARTAAARFWSQVRACARTVRRCFGGLSFGAPSWLAAALILTGS
                     TLSGVCQAQPVKGEATLTVAGGYARLVLKLADDVESEVTTAGTILVIKFRRPIDIPID
                     RVFEAAPDYVTSARRDPDGSAIRLSLARKVTINTMTAGERVFIDMLPDNWKGQPPGLP
                     SEVVRELSERARVAERALRQQRAINEAKKRPPVRIRASVQPTFVRFVFETPDGVGVSS
                     VLNDQKLTLFFNAALTFDLADAKLAAPSNIASINQKVEGETSAVEVMLIGDVDVHSFR
                     EDKNYIIDVAYQQTEKPAAFPSFVAEASHAPATAAPAPTVPARPAPVAAAPERPARES
                     SPSWNSGAIAPPTSELIAEQANIEIKPEAAPKLSPAPEPPMPARPARETAAPAAEAPP
                     KIVVAAPEPPPETKAGEGAVPVIAIRDSDGLRLKFSFATATPAALFRRADTVWLVFDS
                     LKPFDIEPIRSKGGAIIAEVGQLPLDNGQAIRIRLNRPQMPSLTGDAKDGAANWTLTF
                     ADTMQTPPQPLMVIRNVTDPALANVVVPLSKPGLLHRVLDPDAGDTLLVITAPLPIRG
                     FVKRQDFVEMSLLESIHGVAVRPNSDDVTAEIASDKIILGRPGGLTLSAADIGAERAP
                     TAVRPIFDVGEWQKNQTDKFIVQQDALFAAAAGSEPDQRNPARVNLARFYMSRGMYPE
                     AKGILDLALANPRPGPEESLVLTVHAVASILTGRPELGLKDLANPAIGTNNDSQLWKA
                     LGYARLGKWAEAREKFKNVEFAITSLPIELQRIVIMEAMRASLEVRDYSGAAKRGSDL
                     EVVGIPPDLKPAVSVLRGRVAEALGHDKDAQNQYKIAVESTDRAAAAEAKLLDIMLRQ
                     RRDEVGQADALRELETLSVTWRGDWIEVKALQLMARIYSEIGHYGESLAAAKTATRLQ
                     PNSETSRQAQDAAADLFAQLFLGPKGDDLPPVDALGIFYEFRELTPIGRRGDEMIRRL
                     ADRLVAVDLLDQASELLQYQIDRRLEGAARAQVAARLAMVYLTGRKPDRALSALRSTR
                     IADLSGELRQQRLLLEARAQSDIGRHDLALDIITNIVGREAVRLRSDIYWAARRWRQS
                     SEQIELYYADRWRDFKPLNPVEKGDVIRAVVGYSLADDAIGLARFREKYAPLMSGDAD
                     RAAFET"
     gene            complement(3340..3663)
                     /locus_tag="VN968_21860"
     CDS             complement(3340..3663)
                     /locus_tag="VN968_21860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31916.1"
                     /translation="MVNQYLSHSMPKSAGNPALFLTKSSGNGFNKINGLAKQILKSPI
                     LDSNARGARGRPVAVRWLELKDTLEWRERLPLGFGRKSAHARGRSGVALVVCHLARRH
                     GLRPR"
     gene            3878..5254
                     /locus_tag="VN968_21865"
     CDS             3878..5254
                     /locus_tag="VN968_21865"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HXL31917.1"
                     /translation="MELPPLALTQLDKITLSELISALSHALDLTEGQPAGHCVRCSRI
                     GVEIGREIGLGEEEIAELYYALLLKDLGCSSNAARICELYLTDDLSFKQDFKFIDGSL
                     PQALRFVLGHTGLKAGLAERFRAIINIFQNGGEIARELIETRCHRGADIARKMRFSEL
                     VADGIKNLDEHWDGGGKPLGLRGVEIPIYSRIALMAQIIDVFHVSNGPDAALREAQNR
                     SGAWFDPYLVAAFERVAARPAFWDMLRADNRQEAALPQTPVQASVAIDEDYLDDIAAA
                     FAQVVDSKSPYTSGHSERVTLFADMVAEQLGFSAAQRRWLKRAALLHDIGKLGVSNAI
                     LDKAGKLDDNEWAAMRMHAVHSEAILSRIGAFSELAPIAGAHHERLDGKGYPRGLSGD
                     QICLETRIITTADIFDALTADRPYRAAMPITKALSIMSGMVGTQIDADCFTALRRALG
                     RIDETLAA"
     gene            complement(5426..7669)
                     /locus_tag="VN968_21870"
     CDS             complement(5426..7669)
                     /locus_tag="VN968_21870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXL31918.1"
                     /translation="MNLKSFFSLISAMSLAASLACVAAPARADHDGDDRDHGHGDSRD
                     HDHDRDHDGDRDGDHHGRKSPRVVLISLDGAKPDFIQKFIDEGVLPRDGGLARLSRHG
                     AVAVQNVTASPSLTAVSHIAIATGSTAVHNDIPSNTFEAIVAPITSSLSGFAAPIGGY
                     RENPLGPSPQPTASPLWVQLRQQGKNVITATWPGGDGATISINNTVVQPAEPIRVTDY
                     TVPFGAFGGIGAQGFSLSRGAFGSDPAVITALQAAGHFSFSPMLATSAPIETFSCSSA
                     PTATCTNAATLDLKYAIRVAALDTTNDGKVNYDTLVFFDTTRGITAGPFHAPSTGPAF
                     AKFGGENAPFFFEGSGAKVGAAYFVSALAPDLSVVRFARYGANFIPRNTPVLADVDDI
                     NNNIGFWRPQADFRIPERLSPGFTNFPDVEIEAMYEDMVKTFVRYQANIGERAIRNHP
                     DADLVMIYIEQPDGSEHQFLLTDRRQGTNPTDPNSIGANQDPAKVARYASYIRFAYQT
                     ADKAVKQIADAAGRDSNVMVVSDHGFAPFHTSVSMTNILRNAGIDTTKVAIRTSGPAV
                     NIYVNLQNRESGGTVDPATYNALVAQITNAVKNAVDPNPRFNFSLKGGQIFTVVETRP
                     LQCEAPTGLCTSKTIGQDFGDVFALMAPGYNFDGIQNPGVARLGDASFNAATTTLSMP
                     NFYGAHGHDPELPVMSATFIAAGPRIREDTTIRRMRNIDVAPTIMRILGVTPHQVDGE
                     VLHEILR"
     gene            complement(7899..>8261)
                     /locus_tag="VN968_21875"
     CDS             complement(7899..>8261)
                     /locus_tag="VN968_21875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL31919.1"
                     /translation="VADVPQSFKWYQSLLGLAETAPAHDYFGQIVDSDGTVLLCLHQW
                     GAHQHPSLTSPHYAKPGNGLLLFFRVDNFDVALPRARALVSLLEEEPHVNPSTGTTEF
                     SLGDPDGYYVTISAFNPA"
BASE COUNT         1433 a         2600 c         2723 g         1505 t
ORIGIN      
        1 cggtctcgaa ggcggcgcgg tcggcgtcgc cgctcatcaa cggcgcatat ttttcgcgga
       61 agcgcgccag accgatggca tcatccgcca gcgaatatcc gaccaccgca cggatcacgt
      121 cgcccttctc gaccgggttc aacggcttga agtcgcgcca gcgatcggcg tagtacagtt
      181 cgatctgttc ggacgactgg cgccagcgcc gcgccgccca gtagatgtcg gagcgcagcc
      241 ggaccgcttc gcggccgacg atattggtga tgatgtcgag cgcaaggtca tggcgtccga
      301 tgtcgctctg ggcgcgcgcc tccagcaaca gccgctgctg acgcagttcg cccgagagat
      361 cggcaatgcg cgtgctgcga agcgcgctga gggcgcgatc cggcttgcga ccggtcaggt
      421 agaccatggc cagacgtgcg gcgacctgcg cacgcgcggc gccctccagc cggcggtcga
      481 tctgatattg cagcagttcg ctggcctgat cgagcaggtc gaccgcgacc agccggtccg
      541 cgagccggcg gatcatttca tcgccgcgcc gcccgatcgg cgtcagctca cgaaactcat
      601 aaaatatccc gagcgcgtcg accggcggaa gatcgtcgcc cttcggaccg agaaacagtt
      661 gcgcgaacag gtccgccgcc gcgtcctggg cctgccgcga tgtttccgaa ttgggctgca
      721 gtctggtcgc ggtcttggcc gccgcaagcg actcgccgta gtgtccgatc tcggaataga
      781 tccgcgccat gagttgcagc gccttcacct cgatccagtc gccgcgccag gtcaccgaaa
      841 gcgtctcgag ttcgcgcaag gcgtcggcct ggccgacttc gtcgcgcctc tgccgcagca
      901 tgatgtcgag cagcttggct tcggccgcgg cggcccggtc ggtggattcc acggcaatct
      961 tgtattggtt ctgcgcgtcc ttgtcgtgcc cgagcgcttc ggccacccgg ccgcgcaaca
     1021 ccgataccgc aggtttcagg tccggcggaa taccgacgac ttccagatcg ctgccgcgct
     1081 tggccgcgcc ggaataatcc ctgacctcga gcgacgcccg catcgcctcc atgatcacga
     1141 tgcgctgcag ctcgatcggc agcgaggtga tggcgaattc gacgttcttg aatttctcgc
     1201 gcgcttccgc ccacttgccg aggcgcgcgt agcccagcgc tttccacaac tgcgaatcgt
     1261 tgttggttcc gatcgccgga ttggcgaggt ctttcagccc cagctcggga cggcccgtca
     1321 ggatgcttgc gaccgcgtgc accgtcagga caagtgattc ctcggggccc ggtctggggt
     1381 tcgccagcgc gagatcgaga atacccttgg cttccggata catcccgcgc gacatataga
     1441 aacgggcaag attgacgcgg gcggggttgc gctgatcggg ctcggacccc gccgcggcgg
     1501 cgaaaagcgc atcctgctgc acgatgaatt tgtcggtttg atttttctgc cactcgccga
     1561 cgtcgaagat cggccgcacc gcggtcggcg cgcgctcggc gccgatgtcg gccgccgaca
     1621 gcgtcaggcc gccaggcctg ccgagaatga tcttgtcgga tgcaatctcg gcggtgacat
     1681 catcggagtt cgggcgaacc gccacgccgt ggatcgattc gagcagcgac atctcgacaa
     1741 aatcctgccg cttgacaaag ccgcgaatcg gcagcggcgc cgtgatcacc agcagcgtat
     1801 cgccggcatc cggatcgaga acgcgatgca gcaggccggg tttggacaag ggcacgacga
     1861 cattggcaag tgcgggatcg gtgacgttgc gtatcaccat cagcggttgc ggcggcgttt
     1921 gcatcgtatc ggcaaatgtc agcgtccagt tcgcagcgcc gtcctttgca tctcccgtca
     1981 gcgaaggcat ttgggggcgg ttgaggcgga tgcggatcgc ctgaccattg tccagcggca
     2041 actggccgac ctcggcgatg atagccccgc ccttgctgcg gatcggctcg atgtcgaaag
     2101 gcttcaagga atcgaacacc agccagaccg tgtcggcacg gcggaacaac gccgccggcg
     2161 tcgcggtggc aaaggagaac ttcaggcgca ggccgtcgct gtctcgtatg gcgataaccg
     2221 gaacagcgcc ctcgccggct tttgtttccg gcggcggttc gggcgcggcg acgacgattt
     2281 ttggcggggc ttccgccgcg ggcgcggccg tctcccttgc aggacgcgcg ggcatcggcg
     2341 gttccggcgc aggcgacagc ttcggcgcgg cttcgggctt gatctcgata ttggcctgct
     2401 cggcgatcag ttccgacgtc ggcggcgcga ttgcgccgga gttccatgac ggcgagctct
     2461 ccctggcggg cctctcgggc gcggctgcaa ccggcgcggg gcgcgctggg acggtcggcg
     2521 ccggagcggc tgtcgccgga gcatgcgagg cctctgccac gaacgatgga aacgctgccg
     2581 gcttttccgt ctgctgatag gcaacgtcga taatgtagtt cttgtcctcg cggaacgagt
     2641 ggacatcgac atcgccgatc agcatgactt ccaccgccga ggtttcgcct tcgaccttct
     2701 gattgatgga agcgatgttc gatggcgccg ccagcttggc gtccgccaga tcgaaggtga
     2761 gcgccgcgtt gaagaacagc gtgagcttct gatcgttcag cacggaggag acgccgacgc
     2821 cgtcgggcgt ttcgaacacg aagcggacga aggtcggctg caccgatgcg cggatgcgaa
     2881 cggggggacg cttcttcgcc tcgttgatcg cgcgctgctg acgcaacgcg cgttccgcga
     2941 cccgcgcccg ctcggagagt tcgcggacca cctcggaagg aaggccagga ggctggccct
     3001 tccaattatc aggcagcata tcgatgaaca cccgttcgcc ggccgtcatg gtgttgatgg
     3061 tcaccttgcg cgccaacgac agccggatcg ccgagccatc gggatcgcgc cgcgccgagg
     3121 tgacataatc gggcgccgcc tcgaacaccc tgtcgatcgg aatatcgatc gggcgcctga
     3181 acttgatcac gaggatggtg ccggccgtgg tcacctcgga ctcgacgtcg tcggcgagct
     3241 tgagaaccag ccgggcataa ccgcctgcca ccgtcagcgt ggcctcaccc ttgaccggct
     3301 gcgcctggca gacgcctgat aacgtcgagc ctgtcaggat cagcgcggcc gcaagccatg
     3361 acggcgcgcc aaatgacaga ccaccaaagc aacgccggac cgtccgcgcg catgcgcgga
     3421 cttgcgacca aaaccgagcg gcagccgttc gcgccattcc aacgtatctt tcagctccaa
     3481 ccatctgacc gcgaccggac gccctcgcgc cccgcgcgca ttcgaatcca ggattggcga
     3541 tttaaggatt tgtttcgcca acccattgat tttgttgaaa ccattgccgc tgcttttcgt
     3601 caaaaacagc gcggggtttc ctgccgattt gggcatggaa tggcttaaat attgattaac
     3661 catcgcctga aatacgtcgc tacccgcacc ggagcgagcg gcgtttgttg cgctggtttg
     3721 gcgcgacggc gcagtttgtg ggctgcctta ccacgcggct gcgctgcggc gctcagcctc
     3781 caaaacatcc cctgttaatc ctctcatgat ctggtgaggc tttggagatt gcgatccggc
     3841 cgcttctgcg ggccgtcgtt accccggagc ctcgacgatg gaactgccgc cgctcgccct
     3901 tacgcagctc gataagatca cgctttctga attaataagc gcactcagcc acgcgctcga
     3961 tctcaccgag ggtcagccgg ccggccattg tgtgcgatgt agccgcatcg gcgtcgaaat
     4021 cggcagggaa atcggcctcg gcgaggagga gatcgccgaa ctctattacg cgctgctgct
     4081 gaaggatctt ggctgcagca gcaatgcggc gcggatctgc gagctgtatc tcaccgacga
     4141 tctctccttc aagcaggact tcaagttcat cgacggcagt ctgccgcagg cgctgcgctt
     4201 cgtgctgggc cataccgggc tgaaagccgg tctcgccgaa cggttccggg caatcatcaa
     4261 catcttccaa aacggcggtg agatcgctcg cgagctgata gagacgcgct gccatcgcgg
     4321 cgccgacatc gcccgcaaga tgcgcttctc cgagctggtc gccgacggca tcaaaaatct
     4381 cgatgagcac tgggatggcg gtggcaagcc gctaggtctg cgcggggtgg aaatcccgat
     4441 ttattcgcgc atcgcgctga tggcacagat aatcgatgtg tttcacgtct cgaacggccc
     4501 tgacgcggcg ctgcgcgaag ctcagaaccg gtcgggggca tggttcgatc cgtatctcgt
     4561 ggccgccttt gagcgtgtcg cggcgcgacc tgcgttctgg gacatgctgc gtgctgacaa
     4621 tcggcaagag gccgccttgc cccagacgcc ggttcaggca agcgtggcga tcgacgagga
     4681 ctatctcgac gatatcgcgg ccgccttcgc ccaagtggtg gattccaaga gtccttacac
     4741 cagcggccac agcgagcgcg tcacgctttt tgctgacatg gtcgccgaac agttggggtt
     4801 cagcgccgcg cagcgacgct ggctcaaacg tgcagcactg ctgcacgaca tcggcaagct
     4861 cggcgtcagc aacgccatcc tcgataaggc gggcaagctg gacgataacg aatgggccgc
     4921 gatgcggatg cacgccgtgc attcggaggc aatcctgtcg cgtatcggtg cgttcagcga
     4981 acttgccccg atcgccggcg cgcatcacga gcggctggac ggcaagggct atccgcgcgg
     5041 cctctcggga gaccagatct gcctggaaac ccgtatcatc actaccgcag atatcttcga
     5101 tgcgctcacc gccgaccgcc cctatcgcgc ggcgatgccg atcacgaaag ccttaagtat
     5161 catgtccggg atggtcggca cccagatcga cgctgactgc tttacagcgc tccgtcgcgc
     5221 gctcggacgc atcgacgaga ccctggcggc ttaagggggc gcgagacgtt gcgtctcttg
     5281 ggtgacgtgc atgcgccctg tgaccgtcaa tgaagaacgg cattggagca gcctctcgga
     5341 taaaggctga gctgcacaag taggccagtt ccgatgggcg ctggtcagcg cccatcggat
     5401 gagtttgagg atcgcgcgcc ggttttcagc gcaggatctc gtgcagcacc tcgccgtcga
     5461 cctggtgggg tgtgacgccg aggatccgca tgatggtcgg cgccacgtcg atgttgcgca
     5521 tgcgccggat cgtggtgtcc tcgcggatcc gcgggccggc ggcgatgaag gtcgcgctca
     5581 tcaccggcag ctcgggatcg tggccatggg caccgtagaa gttcggcatc gacagcgtgg
     5641 tcgtcgccgc gttgaacgac gcatcgccaa ggcgggcgac gccgggattc tggatgccgt
     5701 cgaagttgta gcccggggcc atcagcgcga acacatcgcc gaaatcctgg ccgatggtct
     5761 tgctggtgca cagcccggtc ggggcctcgc actgcagcgg cctggtctcg accacggtga
     5821 agatctgccc gcccttgagc gagaagttga accgcgggtt gggatcgacc gcgttcttca
     5881 ccgcgttggt gatctgcgct accagcgcgt tgtaggtcgc cggatcgacg gtgccgcccg
     5941 actcgcggtt ctgcaggttg acgtagatgt tgaccgccgg acccgaggtg cggatcgcca
     6001 ccttggtggt gtcgatgccg gcattgcgca ggatgttggt catgctgacc gaggtgtgga
     6061 atggcgcgaa gccgtggtcc gacaccacca tgacgttgct gtcacggccc gccgcgtcgg
     6121 cgatctgctt caccgccttg tcggccgtct gataggcgaa gcggatgtac gaagcatacc
     6181 gcgcgacctt ggcgggatcc tggttggcgc cgatcgagtt cgggtcggtc gggttggtgc
     6241 cctggcggcg gtcggtgagc agaaactgat gctcggagcc gtcgggctgc tcgatgtaga
     6301 tcatcaccag gtcggcgtcg ggatggttcc ggatcgcgcg ctcgccgatg ttcgcctggt
     6361 agcgcacgaa ggtcttcacc atgtcctcgt acatcgcctc gatctcgacg tcggggaagt
     6421 tggtgaagcc cgggctcagc cgctccggaa tgcggaagtc agcctgcgga cgccagaacc
     6481 cgatgttgtt gttgatgtcg tcgacgtcgg ccagcaccgg cgtgttgcgc gggatgaagt
     6541 tggcaccgta gcgggcgaag cgcacgaccg aaagatcggg cgccagcgcc gagacgaagt
     6601 acgcggcgcc gaccttggcc ccgctgcctt cgaagaagaa cggcgcattc tcgccgccga
     6661 acttggcgaa ggccggtccg gtcgacggcg cgtgaaacgg tcctgccgtg atgccgcgcg
     6721 tcgtgtcgaa gaacaccagg gtgtcgtagt tcaccttgcc gtcattggtg gtgtcgagcg
     6781 ccgccacccg gatcgcgtat ttgaggtcga gtgtcgccgc attggtgcag gtcgcggtcg
     6841 gcgccgagga gcacgagaac gtctcgatcg gcgccgacgt cgccagcatc gggctgaacg
     6901 agaagtggcc ggccgcctgg agcgccgtga tgaccgccgg atcggacccg aaggcccccc
     6961 gggacagcga gaatccctgg gccccgatgc cgccaaacgc cccgaacggc acggtgtagt
     7021 cggtgacgcg gatcggctcg gccggctgca ccaccgtgtt gttgatggag atggtagcgc
     7081 cgtcgccgcc cggccacgtc gcggtgatca cgttcttgcc ctgttggcgc agctgcaccc
     7141 acagcggcga cgctgtcggc tgcggcgaag gtcccagcgg gttctcgcga tagccgccga
     7201 tcggtgccgc gaagccgctg aggctcgatg tgatcggcgc gacgatcgcc tcgaaggtgt
     7261 tcgacgggat gtcgttgtgg accgccgtcg agccggtggc gatcgcgatg tgcgacaccg
     7321 cggtgagcga cggcgacgcc gtcacgttct gcaccgccac ggcgccgtgg cggctcagcc
     7381 gcgccaggcc gccgtcgcgc ggcagcacgc cctcgtcgat gaacttctgg atgaaatcgg
     7441 gcttggcgcc gtcgagcgag atcagcacca cccgcggcga cttgcggccg tgatggtcgc
     7501 cgtcacggtc gccatcgtga tcacgatcat ggtcgtgatc gcgggagtcg ccgtggccat
     7561 ggtcgcggtc gtcaccgtcg tgatcggcgc gcgccggcgc cgcaacgcaa gccagcgatg
     7621 cagcgagcga catcgccgat atcaaggaga aaaagctctt caagttcata tggacgcccc
     7681 atgttcgctg cgcaatctgc gcgggtttgg ccgggaatga ggcaccgatt accgggcttc
     7741 gctgacgcgc atgtgacgtc gcggcgacaa cgccgcgaag atgtcggctt ttggcacgga
     7801 agatgtccca tgacccaaca cggacgtgcg ggaacattcc acatgaccgc agtttccagc
     7861 gacacagccc gccggaacct gtgggctcga tgaaggggtt aggccggatt gaacgcactg
     7921 atggtgacgt aatatccgtc aggatctccg agcgaaaact ccgtagttcc agtgctggga
     7981 ttcacatgtg gctcttcttc cagaaggctg acgagcgccc gcgcccttgg caaggctacg
     8041 tcaaagttgt cgacccggaa gaacaacagg aggccgttgc caggcttggc gtaatgggga
     8101 cttgtcaacg agggatgctg gtgggcaccc cactggtgga gacagagcaa gaccgttcca
     8161 tccgagtcga cgatttgccc gaagtagtcg tgggcaggag ccgtctccgc caggccaagt
     8221 agcgactggt accacttgaa gctctggggc acgtcagcta c
//
LOCUS       DATNOI010000539         4400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_954657,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000539 DATNOI010000000
VERSION     DATNOI010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954657"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VN968_21880"
     CDS             complement(<1..690)
                     /locus_tag="VN968_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HXL31920.1"
                     /translation="MKAVGYKKSLPVDNADALIDFVTAKPEPRGRDIRVAVKAISANP
                     VDYKVRKRAAPPEGETKILGYDATGIIDAVGPEVTLFKAGDEVFYAGSIQRQGTNSEF
                     HLVDERIVGAKPKTLSFAQAAALPLTSITAWELLFDRLGAVPGRSVDPRTLLIVGGAG
                     GVGSILIQLARRLTGLTVVATASRPESQKWCLDLGAHAVIDHSKPMKEQIENLKLPPV
                     ALIASLTNTDQH"
     gene            823..1200
                     /locus_tag="VN968_21885"
     CDS             823..1200
                     /locus_tag="VN968_21885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL31921.1"
                     /translation="MKRRNFAHRPGCAVEATLDLIDGKWKGVILYHLQDGTQRFGELR
                     RRMPGITQRMLTKQLRALEDDRLVIRKVYAEVPPRVEYRLSETGESLRPVIDILKAWG
                     EGHQEKLSCAPVSDTVTGPSRAA"
     gene            1285..2679
                     /locus_tag="VN968_21890"
     CDS             1285..2679
                     /locus_tag="VN968_21890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HXL31922.1"
                     /translation="MKFIRTLAVICFSATAAVPAVAQDRRVPTSPAEVKLSYAPIVQR
                     VQPAVVNVYAAKVVQNRNPLLDDPIFRRFFGVPGQQPEQMQRSLGSGVMVDPSGLVVT
                     NNHVIEGADQVKISLSDKREFEAEIVLKDSRTDLAVLRLKDAKEKFPTLDFANSDELL
                     VGDVVLAIGNPFGVGQTVTHGIISALARTQVGITDYQFFIQTDAAINPGNSGGALVDM
                     TGKLAGVNTAIFSRSGGSQGIGFAIPANMVRVVVASAKSGGKAVKRPWLGARLQTVTP
                     EIAETLGLRIPSGALVANVVPNSPAARAGLKLSDLIVAIDGQTIEDLNGFDYRFATRP
                     LGGTSQIDVQRAGKTVKLTIPLETAPDTGRNEIVLAGRSPFQGAKVANISPAVADELH
                     LDADTEGVVVTDLSDNGTAASVGFQKGDIILSVNNQKIAKTSDLEKATRESARLWRIT
                     VVRGGQQINVTLGG"
     gene            2676..4004
                     /locus_tag="VN968_21895"
     CDS             2676..4004
                     /locus_tag="VN968_21895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HXL31923.1"
                     /translation="MSPKRPREGASLFAAAGMEQDAPHPLPDRLRPRALSDVVGQDHI
                     LGPDGALTRMLATRTLGSLIFWGPPGTGKTTVARLLADATELHFEQLSAVFSGVADLK
                     KAFDAARARREMGKGTLLFVDEVHRFNRAQQDSFLPVMEDGTVVLVGATTENPSFELN
                     AALLSRARVLVFHSLDPAAVEKLFANAEKIEGRRLPLDAEARAVLVRMADGDGRAALT
                     LAEEVWRAARDGETFDAEQLQEILQRRAPIYDKSADGHYNLISALHKSVRGSDPDAAL
                     YYLARMMDAGEDPLFLARRVVRMAVEDIGLADPQALVICNAAKDAYDFLGHPEGELAI
                     AQAVIYLATAPKSNAAYKAFGAAMRAAKEGGSLLPPKHILNSPTKLMKSEGYGSGYEY
                     DHDTQDAFSGQDYFPEALGRQTFYDPPDRGFEREIRKRLDYWAKLRRERG"
BASE COUNT          769 a         1381 c         1441 g          809 t
ORIGIN      
        1 gtgctggtcg gtgttggtca ggcttgcgat cagtgccacg ggcggcaatt tcaggttttc
       61 gatctgctcc ttcatcggct tggaatgatc gatgacggca tgcgcgccga gatcgagaca
      121 ccacttctgc gattcgggcc gggacgccgt cgccaccacc gtcagcccgg tgaggcgccg
      181 ggccagctgg atcaggatcg agccgacgcc gccggccccg ccgacgatca gcagcgtgcg
      241 tggatcgacg ctcctgccgg gcaccgcgcc gagacggtcg aacagcaatt cccatgcggt
      301 gatcgaggtc agcggcagcg ccgcggcttg cgcgaacgac agcgtcttcg gcttggcgcc
      361 gacgatgcgc tcgtccacca gatgaaattc cgaattggtg ccctggcgct ggatcgagcc
      421 ggcatagaaa acctcgtcgc cggccttgaa cagcgtcacc tcggggccga cggcatcgat
      481 gatcccggtg gcgtcgtagc ccaggatctt ggtttcgccc tcgggcgggg cggcgcgctt
      541 gcggaccttg taatcgaccg gattggcgga aatcgccttc acggcgacgc ggatgtcgcg
      601 gcctcgcggt tccggtttgg cggtcacgaa atcgatcagc gcgtctgcgt tgtcgacggg
      661 aagcgatttt ttgtatccga cggctttcat gcctgttgtc tccggtctga tgggctcaat
      721 cgtgaaaacg ctcgccgcct aattgtcggt ctggcgctat tttggcaagt actgtaaatt
      781 cagggatata gtccctgttt ggatactatt ggaaaagacc ccatgaaacg gcggaatttt
      841 gcccaccggc cgggttgcgc ggttgaggcg acgctcgatc tgatcgatgg caagtggaaa
      901 ggcgtcatcc tttatcacct gcaggatggc acccaacgct ttggcgagtt gcggcggcgg
      961 atgcccggca ttacccagcg catgctgacc aagcagctcc gtgcgcttga ggatgaccgt
     1021 ctggtgatcc gcaaggtcta tgccgaggtt ccgccgcgtg tcgaatatcg cctgtcggag
     1081 accggagaaa gcctgcgtcc ggtgatcgat atcctgaaag cctggggcga aggccatcag
     1141 gaaaagctat cctgcgcgcc tgtttcggac accgtaaccg gccccagccg cgccgcctga
     1201 tcgggctatt tgccggtccg ggtttctctt ctctcaccct tgcgtcgcgc ttatatctga
     1261 agcgacgaat tcaggatttg ccgcatgaag tttatccgta ccttggccgt gatctgcttt
     1321 tccgcgacgg ccgcagtgcc agccgttgcg caggaccggc gggtgccgac ctcgcccgcc
     1381 gaggtcaaac tctcctacgc gccgatcgtg cagcgcgtgc aaccggcggt ggtcaacgtc
     1441 tacgccgcca aggtggtgca aaaccgcaat ccgctgctcg acgatccgat cttccgccgt
     1501 ttcttcggcg tgcccgggca gcagccggaa cagatgcagc gctcgctggg gtcgggcgtg
     1561 atggtggatc cgtccggtct ggtggtgacc aacaaccacg tcatcgaagg cgccgatcag
     1621 gtcaagattt cgctctccga caagcgcgaa ttcgaggccg agatcgtcct caaggacagc
     1681 cgcaccgacc tcgccgtgct gcgcctgaag gacgccaagg agaaattccc gacattggat
     1741 tttgccaatt ccgatgaatt gctggtgggc gacgtcgtgc tggcgatcgg caatccgttt
     1801 ggggtcgggc agaccgtcac ccacggcatc atttcggcgc tggcgcgcac ccaagtcggc
     1861 atcaccgatt atcaattctt cattcagacc gacgctgcga tcaatcccgg caattcgggc
     1921 ggcgcgctgg tcgacatgac cggcaagctg gccggcgtca ataccgcgat cttctcgcgc
     1981 tccggcggat cgcagggcat tggttttgcg atccctgcca acatggtgcg tgtcgttgtg
     2041 gcctcggcca agagcggcgg caaggcggtc aagcggccct ggctcggcgc gagattgcag
     2101 acggtgacgc cggagattgc cgagaccttg ggactccgca ttccctccgg cgcgctggtc
     2161 gccaatgtcg ttcccaacag tccggcggcg cgggcaggac tgaaactctc cgacctgatc
     2221 gtcgcgatcg acgggcagac gatcgaggat ctcaacggtt tcgactatcg cttcgcaacg
     2281 cgtccgctcg gcggcacctc gcagatcgac gtgcagcgcg ccggcaagac cgtgaagctg
     2341 accatcccgc tcgaaaccgc gcccgatacc ggccgcaacg agattgtcct ggccgggcgc
     2401 tcgccgttcc agggtgccaa ggtcgccaac atttcgccag ccgtcgccga tgaactgcat
     2461 ctggatgcgg acactgaagg cgtcgtggtg accgatttgt ctgacaacgg aaccgccgcc
     2521 agcgtcggat tccagaaggg cgacatcatc ctgtccgtca acaatcagaa gatcgccaag
     2581 accagcgatc ttgagaaggc gacgcgcgag tcggcgcggc tctggcgcat cacggtggtg
     2641 cgcggcggcc agcagatcaa cgttacgctc ggcggatgag tccaaaacga ccacgcgaag
     2701 gtgcaagcct gttcgccgcc gcggggatgg agcaggacgc gccgcatccg cttccggaca
     2761 gattgcgtcc ccgcgcgctg tccgatgtgg tggggcagga tcatatcctc gggccggacg
     2821 gcgccctgac gcgcatgctg gcgacgcgca cgctgggctc gctgatcttt tggggacccc
     2881 ccggcaccgg caagaccacg gtggcgcggt tgctggcgga tgccacggaa ctgcatttcg
     2941 aacagctctc ggcggtgttt tcgggggtcg ccgatttgaa gaaggcgttc gacgccgccc
     3001 gcgcgcgccg cgaaatgggc aagggaacgc tgctgttcgt cgatgaggtg catcggttca
     3061 accgcgcgca gcaggattcg tttctgcctg tcatggaaga cggcaccgtg gtgctggtcg
     3121 gcgcgaccac cgaaaatccc tcgttcgagc tcaacgccgc tttgctgtcg cgggcgcggg
     3181 tgctggtgtt tcattcgctc gatccagccg ccgtcgagaa gctgttcgcc aatgccgaga
     3241 agatcgaggg cagaaggctg ccgctcgatg cggaggcgcg cgcggtgctg gtgcgcatgg
     3301 ccgacggcga cgggcgcgcg gcgctgacgc tcgccgaaga ggtctggcgc gcggcgcgcg
     3361 acggcgagac cttcgatgcc gagcaattgc aggagatcct gcagcgccgg gcgccgatct
     3421 acgacaagtc cgcggatgga cactacaatc tgatctcggc gctgcataaa tcggtgcgcg
     3481 gctccgatcc cgacgcggcg ctatattacc tcgcgcgcat gatggatgcc ggcgaagacc
     3541 cgctcttcct ggcgcggcgc gtggtccgca tggcggtcga ggacatcggc cttgccgatc
     3601 cgcaggcgct ggtgatctgc aacgccgcca aggatgccta tgatttcctc ggccatcccg
     3661 aaggcgagct cgcgatcgcc caagcggtga tttatcttgc caccgcgccg aaatccaacg
     3721 ccgcctataa ggcgtttggc gcggcgatgc gcgcggcaaa ggagggcggt tcattgctgc
     3781 cgccgaagca tatcctgaac tcgccgacca aattgatgaa gtcggaaggc tatggctcgg
     3841 gctatgagta cgaccacgac acccaggacg cgttctccgg ccaggactat ttcccggaag
     3901 cgctgggccg ccagactttt tatgatccgc ccgaccgcgg cttcgagcgc gagattcgaa
     3961 aaaggctgga ttactgggcg aagctgcgtc gggagcgggg ctgacagata gccaacttgt
     4021 tctcggaacc gtctcgtgtc ccggacgcga cgcggcacgc agtgctgcgg cgcagagccg
     4081 ggatccatac gatgtgagcg aggtgggccc cggctcggcg tcgcaccgcg aagcggcgcc
     4141 gcgccgcgtc cggggcacga ggatttgcca gggccgatcg gcgcacaaat agccccgatt
     4201 cgccgtgacg atccctcgct tttacgctac ccaacggctt aatttggagc cgcagccatc
     4261 atgagccgtc gcgttaaaag accattggcg aagtcccgcg tgccgggcga tcgcccgaag
     4321 ggcgcgcgtc cgtaccgggg ctctgccgcc gagcggacgc cggcgcatcg cacgggcggc
     4381 aagtcgccgg cgcggtttgc
//
LOCUS       DATNOI010000540        30399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2025977,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000540 DATNOI010000000
VERSION     DATNOI010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 30399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30399
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025977"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VN968_21900"
     CDS             <1..449
                     /locus_tag="VN968_21900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL31924.1"
                     /translation="VEIDPATGFIRIVNYVIVDDFGVTLNPLLLAGQVHGGAMQGIGQ
                     ALMEQAVYNAKDGQLVTGTFIDYAMPRAADGPSFVFETHNVPCKTNPLGVKGAGEAGA
                     IGSCPAVVNAIIEGLWREYKIDHIDMPATAERVWIAIREHQRRHSL"
     gene            574..1020
                     /locus_tag="VN968_21905"
     CDS             574..1020
                     /locus_tag="VN968_21905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXL31925.1"
                     /translation="MLRTVVVVGTLLLGVGVVAAQQDQVNKTQLAMKSNLKNALALLD
                     IVKGKQPYDQAAVNASLAELEDVGKRFSALFPDSIKGLKPEGDYYASAKVWTDRKGFE
                     AHAASFAKAVGEAKGKIKDLDTLKAAFPAINDECKGCHETYRVKNS"
     gene            complement(1106..1531)
                     /locus_tag="VN968_21910"
     CDS             complement(1106..1531)
                     /locus_tag="VN968_21910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HXL31926.1"
                     /translation="MSKTRIMLATLALGATIALTGSAFAEKMKVTLDSKSEVPPNTSA
                     GTGTADVDYDAASKKLTWKLTYSGLSGPATAAHFHGPAEAGKNAGVKVPIPNATSSPA
                     EGSAILTDEQAADLTAGKYYINVHTAANPGGEIRGQVTK"
     gene            1696..2634
                     /locus_tag="VN968_21915"
     CDS             1696..2634
                     /locus_tag="VN968_21915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXL31927.1"
                     /translation="MSRRIFLAAIIVAAAAFGVFWWLTATPIALAVTEPFHRPNLANG
                     LTAFNAGGCSSCHAVPGQPDRLRLGGGLAIPSPFGTFYAPNISPDPVDGIGRWTEAEF
                     VDAVTKGISPAGFHYFPAFPYPSYQHAKVEDVRDLFAYLQTLTAVSGKVRDHDVPFPF
                     NVRRNIGIWKLLFMDGKPFTPDAAHSAQWNRGAYLVNGLGHCVECHSPRNFLGGIIAA
                     QRFAGGPNPEGEGWVPNITQKGLGDWSARDIADFLETGQMPDGDTAGGPMVRVIKNTS
                     QLSPQDRTAIAEYLKSLPPVDGPPRPKKAAKGDDKP"
     gene            2889..3251
                     /locus_tag="VN968_21920"
     CDS             2889..3251
                     /locus_tag="VN968_21920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL31928.1"
                     /translation="MGDQPVAWCSIAPRETYRPLGGPAADLATERIWSLACLFIKRDH
                     RGKGLTAELITEAIAHAKAHKATIVEAYPVDADSPSYRFMGFVDAFASAGFQEVGRAG
                     QRRHVMRLILSDPALKPR"
     gene            complement(3315..3905)
                     /locus_tag="VN968_21925"
     CDS             complement(3315..3905)
                     /locus_tag="VN968_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31929.1"
                     /translation="MYFASADGQTQLVGYLFAPQSAGPHPAVVMLHGRAGPYSSNVNE
                     ACTLVARNNGSPCNASSLSKRHAAWGIYWADHGYLALLPDSFGPRSKAHGFGRFTHDD
                     PDRDDVNERTVRPLDAEGALKYLQSRKDVASGRIFLQGWSNGASTALNVMYRQASLPS
                     GGFRTAVAFYPGCGPRALIAQDADRIVAGDVLDGDR"
     gene            complement(4114..4359)
                     /locus_tag="VN968_21930"
     CDS             complement(4114..4359)
                     /locus_tag="VN968_21930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HXL31930.1"
                     /translation="MVPFFVQFKCKLGQSYAVANALAEAEIASEIYSTAGDYDLLVKF
                     YVDNDTDIGHFVNEKIQVIPGIQDTHTIITFKAFGTG"
     gene            4505..5305
                     /gene="thiD"
                     /locus_tag="VN968_21935"
     CDS             4505..5305
                     /gene="thiD"
                     /locus_tag="VN968_21935"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HXL31931.1"
                     /translation="MTVPIALTIAGSDSSGGAGIQADLKTFAALGVYGASVITALTAQ
                     NTNGVTAIQQVPRDFVTAQIDAVFGDLDVKAVKIGMVAQLTTIDAIAAGLARWSPKHI
                     VLDPVMVATSGDRLLAADAVEGLRTKLIPRASLLTPNLPEAAALLDEPVAVSEAAIES
                     QGQRLLAIGCAAVLIKGGHGQGAESTDYLVSGNGTVALPAPRIATKNTHGTGCSLSSA
                     IAAGLAKGEELETAVRHAKTWISAAIMAADRLGVGHGHGPVHHFHAFY"
     gene            complement(6294..7850)
                     /locus_tag="VN968_21940"
     CDS             complement(6294..7850)
                     /locus_tag="VN968_21940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL31932.1"
                     /translation="MRYLFEEYAFDTDRRELHRGADVVSIAPQVFDLLDYLIRNRERV
                     VSKDDLISAIWNGRIVSDAALTTRLNVARSAIGDSGEEQRLIKTLPRKGFRFVGPVVE
                     ARGPAGAATADNSIEPPKPALTLPNKPSIAVLPFTNLSGDPEQDYFADGMVDEITTAL
                     SRFKSLFVIARNSSFTYKGRAVDIKQVGRELGVRYVLEGSVRKAAGKVRITGQLIDAM
                     TGAHLWADRFEGDLSDVFALQDEVTVNVVSAIQPKLLQTEMDLAARRPNNLSAYDLCL
                     RAQHQQSWTRGGSAESLRLASRALEIDPRCGFAATLAGECHLRNARHGWTADPKSEIA
                     EGFRFLRLALSIDGNATLALSMLGRATASFLGDFDTAKEMVDRAVALNPNSFRAWEER
                     GWTYITAGQPDEAIRSFERRIRLNPFDPLLFATLAGTSVAFIGLGRFDEAVSAAKKAV
                     RQNPLFPFTYRCLASALAHLGRKAEAGEAVAGLLELEPDFRISERVARGDVLGLRLYI
                     DGLRKAGLPE"
     gene            8014..8256
                     /locus_tag="VN968_21945"
     CDS             8014..8256
                     /locus_tag="VN968_21945"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HXL31933.1"
                     /translation="MSATYGATGLGQTAASARCVSSFFKRYWAAFQERRKRQRLRATL
                     CDLSDRELMDIDTTRGEIDYVASNRGIDPRGIRSAG"
     gene            8515..9330
                     /locus_tag="VN968_21950"
     CDS             8515..9330
                     /locus_tag="VN968_21950"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXL31934.1"
                     /translation="MTDHVEVNRRNWDERAAIHARDATGDYMLDRFRAGEDALHAIEA
                     TELGDISGKRVLHLQCHIGRDTLCLVRRGATVTGLDFSSAALNVARRLSDETGLKADF
                     IEGTVDQAPDLTPGPFDLVFTTWGTICWLPDVKKWARVIASVLAPGGELYFADAHPGF
                     SVLEECAGRFVPTHDFQTPPDRPLQFANETTYTGDPTIMSHQSTREWIHSLSAVLGGL
                     IDAGLTITMFREHEVLPWRGLTSLVPASERMWRLPDGVPRIPLSYSVRAKKGS"
     gene            9659..10954
                     /locus_tag="VN968_21955"
     CDS             9659..10954
                     /locus_tag="VN968_21955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31935.1"
                     /translation="MLVALPRAKRFSASRFTHRGQPPGAEQSTDDRERRSPALADREN
                     SVNEQLTRIRSITAMLYVDIPSVADLKSLAAHRDDICVSIYLPTTPVSQEAAGDRIEL
                     KNLAKEALRQLETANADKRRVAALMDHLDDLVDDDEFWRFQARSLAVLATPDNARTFR
                     VPNALVTIVEVSDRFHLKPLLRAIAFPNTCFVLALAENAVRVIQVSADLPPLMVKVEG
                     LPKDAGSAVGRASVNDRSPSGRIQGSEGQKVLLRQFARKIDHALRGLLTGSDIPLMLA
                     ASEPLASIYRSVNTYAHLATAGIDGSPVHSTDAQLADRARGLLDGLNRDVIAQWKKAF
                     ATRENQGRATTDIAHAARAATYGAVEMMLVDIDEVIPGTVDDTDGTVTFAKSANARDY
                     GVVDEIASRVIRSGGRILGVRKADIPGGKSLAAILRYAM"
     gene            11613..11780
                     /locus_tag="VN968_21960"
     CDS             11613..11780
                     /locus_tag="VN968_21960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31936.1"
                     /translation="MKSSDLDQDVVETAYARWAPIYDANNDAILVERCKVARFGVYTL
                     VCFERGIPSAV"
     gene            11838..12635
                     /locus_tag="VN968_21965"
     CDS             11838..12635
                     /locus_tag="VN968_21965"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HXL31937.1"
                     /translation="MSEECPERRFRTLFISDVHLGARGSQADRLLDFLRSHDADTIYL
                     VGDIVDGWALQSSWYWPQSHNDFVQKMLRKARKGAKIVYVPGNHDEFLRNYYGTHFGG
                     IDVVENTIHEGVDGKRYLVIHGDIFDLVVQNARWLAHLGDKAYDFAIQMNRIVNAFRR
                     WFGVPYWSLSQWAKLKVKNAVNYIGAFEQTLAAEARRHGADGVICGHIHYATIRDEHG
                     IRYMNCGDWVESCTALAEHEDGRFEIITWTDPLRRAAAVTSVAARAA"
     gene            12635..13690
                     /locus_tag="VN968_21970"
     CDS             12635..13690
                     /locus_tag="VN968_21970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXL31938.1"
                     /translation="MRILVATDAWHPQVNGVVRTLTNMAEAARAFGVEISFLTPQSFR
                     TFAMPSYPDLRLALPYQAKIARLIAEARPDSIHIATEGPIGLLVRRYCRKNQLTFTTS
                     FHTRFPEYISARLPIPEAWIWTALRAFHGPSQAVMAATPALAGELRARGFRNVVLWPR
                     GVDASQFHPRVADLGLPRPVFLCVGRVAVEKNLEAFLDLDLPGTKVIVGDGPARAALT
                     RKYPQAVFLGARQGKELAEAYAAADVFVFPSKTDTFGLVLLEALASGLPVAAFPVTGP
                     RDVIGDEPVGALNDDLRIACLTAVTISPQACLAFAARHTWEASARAFVENITNVRAGD
                     PKSDALQFEVKRPRFVA"
     gene            13732..14721
                     /locus_tag="VN968_21975"
     CDS             13732..14721
                     /locus_tag="VN968_21975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HXL31939.1"
                     /translation="MADLSPILPVSPADIDAAARVLAPYAVRTPLLSAAVLDERAGTR
                     VFLKPEMLQRTGSFKFRGAFNRLSSIPMAARGGGVVAFSSGNHAQGVAAAAKILNMQA
                     TIVMPADAPLSKRERTKAYGAEVVLYDRGREDREAIANGIAGKRGATLVRPYDDPFVI
                     AGQGTVGLEIAEDMAALGLTPDIVVAPASGGGLIAGVATAVKARYPKAILMSGEPDAF
                     DDHARSLRAGKREPHRAEGRTICDALMASIPGEMTFAINSRLLAQGITASDAEVAAAV
                     GFAFRELKLVVEPGGAVGLAALLAGRIDGRGKNVVIVLSGGNVDADLFAKLIS"
     gene            complement(14730..15779)
                     /locus_tag="VN968_21980"
     CDS             complement(14730..15779)
                     /locus_tag="VN968_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31940.1"
                     /translation="MVLDAVARVEENLAAQKQAAPDNGLAEALVVIRRAVDEARAAAS
                     GAIDGLALEETLAPVRKGARVIREIAWRWREIGTDGRICDLLDSQVNAIEGACGQIIS
                     TDPKAVLSAAFDLIEAQIAELGESDTASPQAAETAVSRPSPLPTPPGELPAADLEMVP
                     AAAATNIDTATNAPAMPHETLGATEMTAEAADAQALGNYAPDTQAADAQAAYAQAGDM
                     PASDTLDVDAQDDAVLRMVALEMAAPDPSDIDLPADTAHDEIDVTASPPAGPVLVAQP
                     GPTAVPAMSTSLQTSLQPSLQASPEPSLEPSLEPSLGSALLASGLLRKPNASQSDPLA
                     PIRRMSQAEKIAFFS"
     gene            16014..17213
                     /locus_tag="VN968_21985"
     CDS             16014..17213
                     /locus_tag="VN968_21985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL31941.1"
                     /translation="MPPESQIPIASQDTARRFATRLAVFYGTMFGMMGTHLPFFTVWL
                     KAVGIDAFWIGVITAVPPVTRFTVLPFVTALAERRHALRGAIVVTGFATAFWFYIVGT
                     QHQPVLVFLAYAVTCCLWTPMVPLTDAYALRGVARYGLNYGPLRLWGSAAFVAGALVC
                     GLLVDVIAAKNLIWVIASVAALGAFVGLGLQPMGKPKSVTHIQHGAGALLRDRGFLAI
                     IVTSALIQGSHAAYYIFASIAWQQAGLGGLTIAGLWSLGVIAEIVVFALSPRFTLPPV
                     FLVVIAALSAVARWVITAQQPPLAILATVQLAHGLSFGLTQVGTMGLLVRYVPGHVMA
                     RGQGYLAACSGIVSSSVSILSGAIYAQYGQGVYYAMAAMALSGAVLMWLARHRLAGHP
                     QSAASGG"
     gene            complement(17183..18178)
                     /gene="dgcA"
                     /locus_tag="VN968_21990"
     CDS             complement(17183..18178)
                     /gene="dgcA"
                     /locus_tag="VN968_21990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-D-Glu racemase DgcA"
                     /protein_id="HXL31942.1"
                     /translation="MTSTKFPKLAGRIERWPIAGSFTISRGAKTEAVTVVAELNCGGR
                     TGRGECAPYPRYGETPEATLAALLSMQEPLARGLDRWALQAAMPACAARNALDCALLD
                     LEAKSSGQRVWNLLGRPAPEACITAYTISLASPEAMAAATAKAAHRPLLKIKLGGDAD
                     GRRIAAVRQVAPESELIVDANEAWTAENLEQNLAVCAEAGVTLVEQPLPAGRDEALAR
                     IRRPIAVCADESVHDRASLDGLRGRYDAVNIKLDKTGGCTEALAMADAARALGFDIMI
                     GCMVATSLSMAPAMLLTQQARFVDLDGPLLLARDRDGGLRYDGSLVYPPDAALWG"
     gene            18317..19450
                     /locus_tag="VN968_21995"
     CDS             18317..19450
                     /locus_tag="VN968_21995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31943.1"
                     /translation="MNGDPTLERIAQGNGLALCATGSWTARFAPVLEQMVADAERLTG
                     SRPDIFIDVSKVSKLDTFGAWLIERLRRSLAHGGVEARIAGLSANYSSLVDEVRRLKA
                     TAVVDTTSMTITGVLDQIGRNMAGFGTTLIGLIDMLGAVLAAVGRMVIHPSGFRLTST
                     VHHLEQVCWRAVPIVLLITFLIGCIISQQGIFHFRKFGADIFVVDMLGVLVLREIGVL
                     LVAIMVAGRSGSAYTAELGSMKMREEIDALRTMGFDPIEVLVLPRMLALIIALPILAF
                     LGAMAALVGGGLVANFYGGVDPEAFMLRLRDAISIDHFIVGIVKAPVMAAVIGIVACV
                     EGLAVEGSAESLGQHTTSSVVQGIFFVIVMDGVFAIFFASIGM"
     gene            19453..20223
                     /locus_tag="VN968_22000"
     CDS             19453..20223
                     /locus_tag="VN968_22000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31944.1"
                     /translation="MAPEIPDAIIRVRDISVRFGATKVLDGLNLDVKRGEILGFVGPS
                     GAGKSVLTRTIIGLVPKVSGRIEVFGIDLDAANAAARRGVERRWGILFQQGALFSSLT
                     VRQNIQFPVREYLNVSQRLLDEITVAKLGMVGLRPEVAGRFPSELSGGMIKRVALARA
                     LALDPELVFLDEPTSGLDPIGAGDFDELVRTLQRTLGLTVFMVTHDLDSLYTACDRIA
                     VLGNGKIIAAGSMADMQASEHPWLRAYFHGKRARAVMG"
     gene            20243..21124
                     /locus_tag="VN968_22005"
     CDS             20243..21124
                     /locus_tag="VN968_22005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXL31945.1"
                     /translation="METRANFVLIGSFTLAVIAAAFGFVLWFQSLHTTKARSPLRVVF
                     EGPATGLRNGGSVNFNGIRVGEVISVKLDNPRRVVALAMVENNAPIRKDTLVGLEFQG
                     LTGVAAISLKGGEEAAPGVPLDEDGVPVLTADPNALQDVTEAIRGTLQNINRVVADNQ
                     ETVKASLKNLETFTAALARNSERIDNVMLKVDGVVGKADNLMLGLNTLAGGKEGGELF
                     QAVKSIRELAEDFDKRSGALMTDGRRTLGDISRAVNNLDRNPTRLLFGAGNSNAASAP
                     GPGPTPAAAQASERKRQ"
     gene            complement(21799..22692)
                     /locus_tag="VN968_22010"
     CDS             complement(21799..22692)
                     /locus_tag="VN968_22010"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HXL31946.1"
                     /translation="MANDDDYIPLFQYDVALSFAGEDRGIVESIARQLVDCDVKVFYD
                     EFVTHELWGKDLFQHLSAVYRDKAKYCLVFVSKAYKDKVWPKHELRQAQERAFFSSSE
                     YILPVIIEDVELPGLNKTTGFIDVRKTHPMAICGLLLRKLGAFEGSRLDRGELARRKN
                     RTKVRGQKVKFGGAEMISSWPSRIRNSQVMRHLTYNATIKRIPYGAEYVPRLNMRANC
                     KDCGVRRGQLHVPGCDVERCPICTGQLLSCDCPIEGYTSEPFAAKLLTGKEPDLVEAK
                     DFTPDRFQSRTEMSKRTRKKR"
     gene            complement(22682..22975)
                     /locus_tag="VN968_22015"
     CDS             complement(22682..22975)
                     /locus_tag="VN968_22015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31947.1"
                     /translation="MIYLSVGSAGLHADPRDGSGHVPVEDGPTKMMMAFMERVGGFIV
                     VRRPEYAKNELLKLNLQIGGYDEKSTHPYFFRTLKKSPVRVMPFTRKRSAHGK"
     gene            complement(23042..28009)
                     /locus_tag="VN968_22020"
     CDS             complement(23042..28009)
                     /locus_tag="VN968_22020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXL31948.1"
                     /translation="MANNPKKAKDPTEVALSAIQEALNISDAPIDGNRGSVRSDATST
                     IASPMPDYSQSPFDTRPSDDRPISEEPQRPIRRAANDDRETIGQILQAIQKGRPARNV
                     YTLASIFAAIWVLGAALLTASFLSSLQAAVGQGSGILVLAGLSAMFFAPVLLFYFLAS
                     LSWRGQELRMIAQSMAQVAIRFSEPEGAASDSMVTVGQAIRREVAAMGDGVERAIARA
                     GELETLVANEVSALERAYSDNEVRIRALLQDIAHQRDNLVGQAEQVRSAISGVQIDLR
                     HDIALISDAIASRVDEVAKSITGALEERGAHITSALSNAGDNMILALGERGGDLLDRL
                     EEASAETTRAVLDASERLTTSLNFKTGHVHDEFVDLADRVHEMLNERIDRITSEFEHR
                     SSTIVDGISERTEQVHDSLKNSSDSLLLELELRSGDLVNKIDEAGNRLATNILTSGDK
                     ASEALDVTVNSLVAKVVSQTETAHDTLSLQMSAFDELVKNQGGELAEKFARDSGTLGA
                     LITRHISEFDRTVKTFGGEIVERMGQRTQDISENLKTYVDTFDTRLTSNGGEITASLD
                     QRLLQFETTLETRVANLDTSLDSKIRSFDESVGGHLKSLEQTFDTRAKSVTETIDNRL
                     GTLASSLTDGAAQAIYSIDSRLTKLTSSLSDGTAQAIEAVDRRISNVTETIDGRSAQL
                     TNTITARFQEIHQGIESRVGAVATDIDMRVSQFEDLLGSRVEAVAGRIESSGRQASDD
                     LMARAEELSLGIKSHVEDAERSLTNLVVNTSETIQTGARAAQQSLLTVSSDVGAQLKM
                     TSAEVERALTAVGSGAANSILSSAREAQTTLTTASGEAAVQIKSLSADVERTLSAAGT
                     ATASSVVAGAREAQTTLVSASADAASQVKSLAADVERSLSAAGTATASSVVAAAREAQ
                     STLVAASSEAASHVKSLAADTAASILGSAREAHLSLTATSGEAANQIKAITADIERSL
                     GAVTASTTDNIQTSALNAQIALVAVSNEVSSKVKSTSADVERSVLAASGTFSSTMTGK
                     TDEIVTYVQQQTDRLAQMIDGKRGSLVEAIGAKASQLTVDMDRVTSDSLKSIETRGLA
                     FSQSMAANGSDVARSITSAGEIATTAVSKSLKELEQASRTAIDQSRQVSIAAVTEMQE
                     TSKILRTDTVALFERLREGNILLQEVLTGAHDNLNSLERALVTRVADFVSAMNDVTSR
                     NGVATQTLEDQLSIFNTKTSRALEDLGSLSSQFDSHGKALVEAAGLVEQSNRDTSLSV
                     AERKAELESLVTTIDLRTADLDHRLSRFTGLLDESLAAAESRARDIARVVAETAGAGS
                     AAISRQFESVRASAEEERRLTTDAMSEMYQQTTHEADAMFKQSADKFASMVSSMKQMA
                     LEMHNELEATRNELRRGVLEMPQEAAESTSQMRKVIVDQIEALAELNRIVARHGRGLD
                     VVSAGRTSAARDEEPVHAVAAAGRSEAPAPVRAAPPRPREAASSASSLPPPDLGAPAS
                     RRTEAPPVSPANPEQGRDGWLSDLLNRADVPGSSREAPRGRAPQTPGVGANPLESLSL
                     DIGRLMDRNLASEMWDRYQRGESKAFTKRLYTPAGQKAFDEVGRKYRADRTFKQTVDR
                     YITEFERLLDEIARDDRGPAALRSHLVSETGMVYTLLAHAAGRLG"
     gene            28356..28733
                     /locus_tag="VN968_22025"
     CDS             28356..28733
                     /locus_tag="VN968_22025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HXL31949.1"
                     /translation="MALHLERIDWMPSPPLVPDDGPIDIEHLRRMTLGDASLEHEVLA
                     MFSAQAVGLIGALAMLPSDAGALAHLLKGSARAIGAFQVADAAGRLETAIRTGDDRSE
                     PFAELDDAVAQARTAIDAILRRS"
     gene            28882..29202
                     /locus_tag="VN968_22030"
     CDS             28882..29202
                     /locus_tag="VN968_22030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXL31950.1"
                     /translation="MAKIHFVDHSGETRTIDVENGATVMEAAIRNAIPGIEAECGGAC
                     ACATCHVYVDEAWREKVGAPSPMEEDMLDFGFDVRPNSRLSCQIKVSDALDGLVVTTP
                     ERQA"
     gene            complement(29253..29709)
                     /locus_tag="VN968_22035"
                     /pseudo
     CDS             complement(29253..29709)
                     /locus_tag="VN968_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
     gene            29882..>30399
                     /locus_tag="VN968_22040"
     CDS             29882..>30399
                     /locus_tag="VN968_22040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL31951.1"
                     /translation="MSEAIKTDVLIIGAGPCGLFAVFELGLLDIKAHLVDILDKIGGQ
                     CAELYPEKPIYDIPGIPFVTGQGLTEALLQQIKPFNPTFHLNEMVETIEKIGNPGFRV
                     TTDAGQVFECKVVVISAGGGSFQPKRPPVPGIEAYEGTSVFYSVRKMEQFRDKDLLIV
                     GGGDSALDWTLNL"
BASE COUNT         5545 a         9213 c         9665 g         5976 t
ORIGIN      
        1 aggtcgaaat cgatcctgcc accggtttca tcaggatcgt caactacgtc atcgtggacg
       61 atttcggggt gacgctgaat ccgctgctgc tcgcgggcca ggttcacggc ggcgcgatgc
      121 agggcatcgg ccaggcattg atggagcagg cggtctacaa cgcgaaggac gggcagctcg
      181 tcaccggaac gttcatagat tatgcgatgc cacgcgcggc cgacggtccg tcattcgtgt
      241 tcgagaccca taacgtgccc tgcaagacca atccgctggg tgtcaagggc gcgggcgagg
      301 ccggcgcgat cggctcctgt ccggcggtgg tcaacgcgat catcgagggg ctgtggcgcg
      361 aatacaaaat cgatcacatc gacatgcccg cgaccgcgga acgggtctgg atcgccatcc
      421 gcgagcatca gcggagacat agcctctgat atttgccaca ttgccacgga atgaaacacc
      481 gcctgcggtg acgggaatga ggattgctgc agcgcgcatc aagcgcatcc tgcggcgtta
      541 cctggaaacc cctgagaaac ggagaactgg ccgatgcttc gaactgtcgt tgtggtagga
      601 acgttgttgc ttggtgttgg cgtggtggca gcccagcagg accaggtcaa caagacccag
      661 cttgcgatga aatccaatct caagaacgct ctcgccctcc tcgatatcgt caaaggcaaa
      721 cagccttatg accaggccgc ggtcaacgcg tcgctggccg agctcgagga cgtcggcaag
      781 cgtttttcag cgttgtttcc ggacagcatc aaaggcctga agccggaagg cgactactac
      841 gcttcggcga aagtctggac cgacagaaag ggcttcgaag cccacgcggc gagctttgcc
      901 aaggcggttg gcgaggcaaa gggcaagatc aaggatctcg ataccctcaa ggcagcgttc
      961 cccgccatca acgatgaatg caagggctgc cacgagacct accgggtcaa gaacagctga
     1021 cgcgggaacg accaaagaaa aaagagggcg gacacgtgat gcgtccgccc cttgatctaa
     1081 atacccgtca aacgaaaggt cgcgattact tggtcacctg gccgcggatt tcgccgcccg
     1141 gattggctgc ggtgtggacg ttgatgtagt acttgccggc cgtcagatcg gcggcttgtt
     1201 cgtccgtcag gatcgcgctg ccttcggccg ggctcgaggt ggcgttcggg atcggaacct
     1261 tgacgccggc gttcttgccg gcttcggccg gcccatggaa atgcgccgcg gtcgccgggc
     1321 cggatagccc cgaataggtc agcttccagg tcagcttctt ggaggcggca tcgtagtcga
     1381 cgtcggcggt gccggtgccg gcactggtat tgggcggcac ttcggatttg ctgtcgaggg
     1441 ttactttcat cttctcggca aacgcggatc ccgtgagggc aattgttgct cccagcgcaa
     1501 gcgtagcaag catgatcctg gttttcgaca tggtttcctc ctgttggcgc caagtggtgg
     1561 tgccagattc gaaacaacga tttgtcggtt ttattcccgt gacgggcttg atcgcgccaa
     1621 attttcaaag agcccacggc cggaatagcc gccatactcg ccatcgaatc gaaacggaca
     1681 gagacggatc ggtgaatgtc gcgacgaatt tttctggctg ccatcatcgt tgccgccgca
     1741 gcctttggcg tgttctggtg gctaacggca accccgatcg cgttggctgt taccgagccg
     1801 ttccatcggc caaatcttgc caacggactg acggcattca acgccggagg atgttcctcc
     1861 tgtcatgccg tgcccggcca gcccgatcgt ttgaggctcg gcggcgggct cgcgatcccg
     1921 tcgccgttcg gcaccttcta cgcgcctaac atctcgcccg atccggtcga tggcatcggg
     1981 cgctggaccg aggccgaatt cgtcgacgcg gtaacaaaag ggatttcgcc ggccggattt
     2041 cattacttcc cggcctttcc ctatccgtcg taccagcatg cgaaagtcga agacgtccgc
     2101 gacctgttcg cttacctgca aacgcttacc gccgtgtccg gcaaggtgcg cgatcacgac
     2161 gtgccgtttc ccttcaatgt ccggcgcaat atcgggatct ggaaactgct gttcatggat
     2221 ggcaagcctt tcacgccgga tgccgcgcac tctgcgcagt ggaaccgcgg tgcctacctg
     2281 gtcaacggcc tcggccattg cgtcgagtgc cacagtccgc gcaattttct cggcggcatc
     2341 atcgcagcgc agcgcttcgc cggcgggcca aacccggaag gcgagggctg ggtgccgaac
     2401 atcacgcaaa aggggctggg cgactggagc gcgcgggaca tcgccgattt cctcgaaacc
     2461 gggcaaatgc ccgatggcga caccgccggc ggtccgatgg tgcgcgtgat caagaacacc
     2521 tcgcaactaa gcccccaaga tcggacggcg atcgccgaat atctcaagtc gctgccgccg
     2581 gtcgacggac cgccgcggcc caagaaggcg gcgaagggcg acgacaagcc ctagtggaca
     2641 tgtttttact ttcatcgtag caagtcgatc ggagggcccg cgtgaatcaa ctccatttcc
     2701 gcaacgtcga cgccgcttgc tggacggact tcgcgcgtct ttttgaggag cgtggcggcc
     2761 cgaagagctg ttggtgcatg gtttggagag cttcgtcggc cgaagcgaaa tgcaaggacg
     2821 gcagcaaccg tcgggctcag atcaagtcga gagtcgatgg cgggataccg atcggcctga
     2881 tcggctatat gggcgaccaa cccgtggctt ggtgttccat cgcaccgcgt gagacatacc
     2941 gcccactcgg cggacctgca gcagaccttg cgacggagcg gatctggtcg ctcgcgtgct
     3001 tgttcatcaa gcgggatcac cgcggcaagg ggctcaccgc agagctgatc acggaggcca
     3061 tcgcccacgc caaggcacac aaggccacga tcgtcgaagc ctaccccgtc gatgcagact
     3121 cgccgagcta ccggtttatg ggatttgtcg atgccttcgc ttccgcagga tttcaggagg
     3181 tcgggcgagc cggacaacgc cgccacgtca tgcgtctgat tctgtcggat ccggcgttga
     3241 agccgcgttg aagcagggta cattccagag gtgctggaaa atcgtaatgg gcggcaacaa
     3301 gcgctgcaac ggcatcagcg gtcgccgtcg agtacgtcgc ctgcaacaat ccggtccgcg
     3361 tcctgcgcaa tcaaggcgcg aggaccgcat cccggataaa atgccaccgc ggtgcgaaag
     3421 ccgccggagg gcagggaggc ttgccggtac atgacattga gcgccgtgct cgcgccgttt
     3481 gaccagccct gcaggaagat tcggccggat gcgacgtcct tgcgggattg cagatatttc
     3541 aatgcgcctt ccgcatccag cggacgaacg gtgcgctcgt tcacgtcgtc gcggtccggg
     3601 tcgtcgtgcg taaaccgtcc gaaaccgtgc gctttgctgc gaggcccaaa actgtcgggc
     3661 agcaacgcca gatacccgtg atcggcccaa tagatgcccc acgccgcgtg acgtttggag
     3721 agcgacgagg cattgcaagg cgagccgttg ttccttgcaa cgagcgtgca tgcttcgttg
     3781 acgttggaag aatacgggcc cgctctgcca tgcaacatga cgacggcggg atgcgggccc
     3841 gcgctctgcg gagcaaacaa ataacccacc agttgggtct ggccgtcggc gctggcaaag
     3901 tacactgttt ccggctccgc acgtgcatcg gtgacgacaa ggcttccgac acaggcacct
     3961 acaatcgtcg cggctccgac agaatcgaaa aatccccgcc accgcatgct gccgcctctg
     4021 atcgtcgcga agtgctcgaa tgcgggttcc gcatcacccc gaggaagccg gggtcgtccg
     4081 gctgctttaa caggctttcg ccccatccgc gcgctagccg gtgccgaagg ccttgaaggt
     4141 gatgatggtg tgggtgtcct ggatgccggg aatgacctgg atcttctcgt tgacgaaatg
     4201 gccgatgtcg gtgtcgttgt cgacgtagaa cttgaccagc aggtcgtaat cgcccgcggt
     4261 ggagtagatc tccgaggcga tctcggcctc ggccagcgcg ttggcgacgg cataggactg
     4321 gccgagcttg catttgaact gcacgaaaaa gggaaccatt gctgtcgtct ccgaagggcg
     4381 tcgcgtccag cccccaatag gccaaatctg gcggacgatg gcaagcgaac ttgccggcaa
     4441 cagcgcgtcg gtctaggaca acgtatggcc ccagtggggc agcaagattt ccgcgggcag
     4501 catgatgacg gtaccgatcg cgctcaccat cgccggatcg gattcctctg gtggcgccgg
     4561 tatccaggcg gacctgaaga cgttcgccgc gctcggcgtc tacggcgcgt cggtgatcac
     4621 ggccctgacc gcgcagaaca caaacggcgt caccgccatt caacaggtgc cccgggattt
     4681 cgtgaccgcg caaatcgatg cggtattcgg cgatctcgac gtcaaggccg tcaagatcgg
     4741 catggtggcg cagttgacga cgatcgatgc catcgccgcc gggttagcgc ggtggtcgcc
     4801 aaaacatatc gtgcttgatc ccgtgatggt ggcgacctca ggcgaccggc tgctggcggc
     4861 ggacgctgtc gaaggtttgc ggacaaaact cattccgcgc gcctcgctgc tcacgccgaa
     4921 cctgccggaa gcggccgccc tgctggacga gccggtcgcc gtcagcgagg ccgcgatcga
     4981 gagccagggc cagcggctgc tggcgattgg ttgtgccgcg gtgctgatca agggcgggca
     5041 cggacagggc gccgagagca ccgactatct cgtcagcggc aacggcaccg tcgccctgcc
     5101 ggcgccacgc atcgccacca aaaacaccca cggcaccggc tgttcgttat cttccgccat
     5161 cgccgcgggc cttgccaagg gcgaggaact ggaaaccgcc gtgcgccacg ccaaaacatg
     5221 gatcagtgcg gcgatcatgg ctgccgatcg cctcggcgtc ggccacggcc atgggccggt
     5281 ccatcatttt catgcgtttt attgaggcag cgctcataca tcgccccggc gaacgccggg
     5341 ggcacataac ccctggcgtt gattgtcgcg aaaggcctcc tcggctccgt cgtttaaatc
     5401 gataggacct ggaaggacat catgatggcg ttgccgggca ccgggccgtg atcgagcgtc
     5461 atctcggctc gcgtgcacgg ccatcggtga tcagggcggt cacgccgcgc ttgaccgggg
     5521 acaggcatgc caaatccggt tccaattggg aagaatcgga aaattaagca tacggatctt
     5581 gccggcggag cgggccgcgt caaatgcgtg acaagtcgga gatcttgagc gaaaaagaac
     5641 taggacaatt gggtggcgac ttcgcagtag cgcatctatc tactaggggc gagggatggt
     5701 cggccgtcaa agatttacct cagaattgac cgatcttcct atttgcaaga cggtggcgtc
     5761 gtgcggagcg ggagggtttt cgacatgcgc ggaatgttgc cgccgagcga agctcggatg
     5821 cggatgtgcg tgatgcaggt cggcaagcgc gggcgctgga cataccggga ctgggcaaga
     5881 tggcgctttt tgtggaagtt ctttccctgg tacgcgcgcg aattgcgtta tcggggagat
     5941 ttctgcatcg gagcggcctg catgcactgg ttctgggagg actccgataa gaaggaacgg
     6001 ggttattgtt cggcagcggt ctcgcggacg cagaacatgc tgtgggcgaa ggaattggag
     6061 gagggtgacc aacaagctcc tgaaccgcga agccttcgcc ggggcggata gctcgggcgg
     6121 cgcggcatgc cgcacgggcg catcggccat tggctagtca gcgaaccgac tgtttcttcc
     6181 tgccactttg tccgcccaac caatatctgg agcaacggga cgtttgatgg gtaacggtcg
     6241 gtgcgattgg ggcatgtact cataagtcag gggccgcgga tcagggcgcc gcgtcactct
     6301 ggaagtccgg ctttgcggag gccgtctata tacaaccgaa gccccaatac atctccgcgc
     6361 gctacccgct cagaaatgcg aaaatcgggt tcaagttcga gcagaccggc cactgcctcc
     6421 cctgcctctg ctttccgtcc tagatgagcc agggccgacg caaggcagcg ataagtgaaa
     6481 ggaaaaagcg ggttctggcg gacggccttc ttggctgccg agactgcctc atcgaaacga
     6541 ccaagaccga tgaatgcaac gctcgttcct gcaagcgttg caaaaagcaa cgggtcaaat
     6601 gggtttaatc gaatgcggcg ctcaaagctc cggattgcct cgtcgggctg ccccgctgtt
     6661 atgtaagtcc aacctcgctc ttcccatgcg cggaatgaat tagggttcaa ggcgaccgca
     6721 cggtccacca tctcctttgc agtatcgaaa tcgccaagaa aggaagcggt tgcgcggcca
     6781 agcatgctca atgccaaagt ggcgttcccg tcgatactga gcgccaaccg gagaaaccga
     6841 aatccttccg caatttctga cttcggatcg gccgtccatc cgtgccgtgc atttcggagg
     6901 tggcattccc ctgcgagggt agcagcaaag ccgcaccggg ggtcaatctc caatgcgcgc
     6961 gaggcaagcc gaaggctctc ggccgatccg ccccgcgtcc atgactgttg gtgttgtgct
     7021 cgaagacaaa ggtcgtaggc gctgaggttg ttcggtcgac gcgccgccaa atccatttct
     7081 gtttgaagca attttggctg aatggcagaa acaacattca ccgtcacctc gtcctgcagc
     7141 gcaaaaacat cgcttagatc accttcaaat cgatcagccc aaaggtgcgc gccggtcatg
     7201 gcatcaatca attggccggt aatgcggact tttccggccg ccttgcgcac acttccttcc
     7261 aggacgtagc gaacgccgag ttcgcgcccg acctgtttga tatcgacggc ccttcctttg
     7321 taagtaaagc tcgaattgcg cgcgatcacg aatagagatt tgaaccgcga caaggcggtg
     7381 gtgatttcgt ccaccatgcc atccgcgaaa taatcctgct ccggatcacc gctcagattg
     7441 gtgaagggga gaaccgcgat tgagggttta ttggggagcg tgagggccgg tttcggcggt
     7501 tctatcgagt tgtcggcggt tgccgcgccc gccggccccc gcgcttccac cactggtccg
     7561 acgaaacgga agcccttgcg cggcagtgtt ttgatgaggc gctgttcctc gccggaatcg
     7621 ccgatcgcgc ttcgggcgac attcaggcgg gtcgtcaggg ctgcatcgga gacaatacgc
     7681 ccgttccaaa tggcgctgat gaggtcgtct ttactgacga cacgctccct gttgcggatc
     7741 aggtaatcga gcaggtcgaa tacctgcggt gcgatggaga cgacgtccgc cccgcgatgc
     7801 agctcgcgcc ggtcagtgtc gaatgcgtac tcctcgaaga gatagcgcaa gctgccaatc
     7861 cctttgggcg gcctccgatc atcggggctc cagcatcgct tggaccgcaa agaataagcc
     7921 gccggtgagg aaaatgtaag ccgaatgtca agcgtgccct cccatgccct ggcaccctcg
     7981 tctgggtaga aacaacaccc aggagatgcc gtgatgagcg cgacctacgg tgcaacaggg
     8041 ttgggacaga ccgccgcatc ggcccggtgc gtctccagct ttttcaagag atattgggct
     8101 gcgtttcagg aacggcgcaa acgccagcgg ttacgagcca ccttgtgcga cctaagtgac
     8161 agggagctca tggatatcga tactacgcgc ggcgagatcg actacgtcgc ctcaaaccga
     8221 ggtatcgatc cgcgaggcat ccgatccgcc ggatgaccgt ctgatatccc ggcaccgggt
     8281 ggttggccgg aattgaacac gaccaggcgc gccatcccct gggccgcctt ccggtatcgg
     8341 tcaatcgcgt catttcaacc gtgcgccgat gattttcggt ctacccggat aaacagttca
     8401 gggtccgttg acatgtctca aaggtgccaa ttccggaagt gaccgtctcg aaaaagatga
     8461 aggcgaagcc cgccgctgct tgcgcggctt ttgcatatgc cacatagagt tcacatgacc
     8521 gaccacgtcg aagtgaaccg ccggaactgg gatgagcgcg ccgcaattca tgcacgcgac
     8581 gccactggtg attatatgct tgaccggttt cgcgcaggcg aggacgctct ccatgcaatt
     8641 gaagcgaccg aactcggtga catttccgga aagcgcgttc tgcatctgca gtgccatatt
     8701 ggtcgggata cattgtgcct ggtacggcgc ggcgcgactg ttaccggact cgatttttcc
     8761 agtgccgcgc tgaatgttgc gcggcgtttg tccgatgaga ccggcctgaa ggcagacttc
     8821 atcgagggga ccgtagatca agccccggat ctaacgcctg gcccgttcga tctcgtcttc
     8881 acaacgtggg gaaccatttg ctggctgccc gatgtgaaga aatgggcaag ggtcattgcg
     8941 tctgtgctgg cgccgggcgg ggagctttac ttcgcagacg ctcatccggg ttttagcgtg
     9001 ctggaagaat gcgctggaag gtttgtgccc acccatgatt ttcaaacgcc tcccgaccgg
     9061 ccgctgcagt tcgcaaatga gaccacgtac actggcgacc cgacgatcat gtcgcaccaa
     9121 tcaacacgag agtggataca ctcgctctcg gccgtcctcg gcggactcat tgatgccgga
     9181 cttacaatta cgatgtttcg tgaacacgag gttttgcctt ggcggggtct gacaagcttg
     9241 gtgccggcgt cggaaaggat gtggcggctt ccggacggag tgcctcgcat tcccttgtct
     9301 tattcagtaa gggcaaaaaa aggctcgtga aaatcggggc cttgcgtggc tgagagccac
     9361 caagtccggt tgggtcatta gcgtcgttcc agcggccggt cggtcacttc cggtctaccc
     9421 cgatgaacag acattctcgg ggttcgtccg caggtcgcaa aggtgccgat ttcagacatt
     9481 caatggactc cgagtaggca cggctcagac tatgcagttc gacgaagttg ccgagcgcca
     9541 aacaaacgct gacaaaaccg gcgacagttc ccaacgaccg gctgcacctg cgacgacatt
     9601 tccgccaacg aggatttgag gtatattgct aacgtgtcat ctggcagcct gccgaaggat
     9661 gcttgtcgct ttgcccaggg cgaagagatt ctcggcaagt cgatttacgc atcgtgggca
     9721 gccgccaggt gcagaacagt ctaccgatga ccgggaacgc aggagcccgg cactagccga
     9781 tcgggaaaat tccgtcaacg aacagctgac ccggatacgg agtatcactg ccatgcttta
     9841 tgtcgacatc ccgagtgtag ccgatctgaa gtcgctggcg gcccaccgcg acgacatctg
     9901 tgtctcgatt tatctgccca ccacgcctgt cagccaggag gcggcgggtg accggatcga
     9961 attgaagaat ctcgcgaagg aggccttgcg ccagctcgag acagcaaacg ccgacaagcg
    10021 tcgcgtcgcc gcgctcatgg atcaccttga tgacctggtc gatgatgacg agttctggcg
    10081 attccaggcg cgcagtctcg cggtgctcgc aactccggac aacgcacgga cgtttcgcgt
    10141 gccgaacgcg ctggtaacaa tcgtcgaagt atcggaccga tttcacctca agccgctgtt
    10201 acgcgccatc gcatttccca atacctgctt tgtgctggcg ctggccgaaa acgcggtccg
    10261 cgtcattcag gtctccgccg acctaccgcc cttgatggta aaggtcgagg gcttgccgaa
    10321 ggacgctggc agcgcagtcg gccgcgcctc cgtcaatgac cgctcgccga gcggtcgtat
    10381 tcaggggtcg gaaggtcaga aggtcttgtt gcgccaattc gcccgcaaaa tcgatcatgc
    10441 gctgcgcgga ttgttgaccg gcagcgacat cccgctgatg ctggccgcga gcgagccgct
    10501 agcctccatc tatcggtcgg tgaacaccta cgcccatcta gccacggcgg ggatcgacgg
    10561 cagccccgtg cactcgaccg atgcccaact cgcggatcgg gctcgcggtt tactggacgg
    10621 cctgaatcgg gacgtgattg cccaatggaa gaaggcgttc gcgacgcgcg aaaaccaagg
    10681 tcgagcgacg actgacatcg cacatgccgc gcgggctgca acctatggcg ccgtggagat
    10741 gatgctggtt gatatcgacg aggtcattcc cggtacggtc gatgatacgg acggcaccgt
    10801 caccttcgct aaatcggcga acgccaggga ctacggagta gtcgacgaaa tcgcgagtcg
    10861 tgtcatccgc tcgggcggcc ggattcttgg cgtgcgaaag gccgatattc cgggtggcaa
    10921 atcgctcgct gccattttgc gctatgccat gtaagttaaa tgaaagggta catctctggg
    10981 aactggcggg gtctatgata tttgctattg gcaacattct gcgcgggaac gtcgaagtcc
    11041 gaaatgaaga acggacgccg tcagctagat cggcgatgtc cgaaaaatgc cacaggacta
    11101 aaccgctcgc gcggtagagc gctgcggcaa gttgcgcggt cgggagagat caacggcgcg
    11161 tcctgtctga agatgaggag ttgcaaccct cactccagac aggagcatct gccgaccaac
    11221 gaggcgctga acccgcaacg ctgatgtgcc atcgaagaca aaatgatctg caactcgggc
    11281 ccgaaggtgc aacacgacta cattggcgcc gccaaggatt cgaataatga aatacgcaca
    11341 gtctgagatg cggtgattat cttgaccagc agatcgagac agtttcagtt tgcatgagtt
    11401 gagttttttc gactctctct acgatcggtt cgcgctatgc ggctccgtcg ttcaaatcaa
    11461 tcgggcacgg agcatggttc cctgcgttcg ccgggacttc tcgtggagaa gccaacatcg
    11521 cttctgtcgc ttggttgtcg tacggcgctg ctatttcgca atcctgcagg cagacgctcg
    11581 attcgtggtg cgcgtgcttg cctggatgac gcatgaagag ttcggatctc gaccaagacg
    11641 tcgtcgaaac cgcctatgca cggtgggcgc cgatctatga cgcgaacaac gatgccatcc
    11701 tcgtcgaacg gtgcaaggtc gcgcggttcg gcgtctacac gctggtctgt ttcgagcgcg
    11761 ggattccgtc ggcggtgtga caccgtcatc acgctgtcac gggattccgg tatctgcttg
    11821 cgcatgggaa ttacgctatg agtgaagagt gtccggaacg gcgctttcgc actttgttta
    11881 tctcggacgt ccatctcggc gcccgcggct cgcaagccga ccggttgctg gacttcctcc
    11941 gcagccatga tgccgatacc atctatcttg tcggcgatat cgttgatggc tgggcgctgc
    12001 aatccagctg gtactggccg caatcgcaca acgacttcgt gcagaaaatg ctgcgcaagg
    12061 cgcgcaaagg cgccaaaatc gtctatgtgc cggggaatca tgacgagttc ctgaggaact
    12121 attacggcac tcatttcggc ggcatcgacg tcgtggaaaa caccatccat gagggggtcg
    12181 acggcaagcg ctatctcgtg attcacggcg atatcttcga tctcgtggtg cagaatgcgc
    12241 gctggctcgc ccatcttggc gacaaggcct acgatttcgc catccagatg aatcgcatcg
    12301 tcaacgcgtt tcggcgctgg ttcggcgtgc cctattggtc gctgtcgcaa tgggccaagc
    12361 tgaaggtcaa gaacgccgtg aactatatcg gcgccttcga acagaccctg gccgccgagg
    12421 cgcggcgtca tggcgccgat ggggtgattt gcggccacat ccattacgcc accatcaggg
    12481 acgagcatgg catccgctac atgaactgcg gcgactgggt ggaaagctgc accgcgctcg
    12541 ccgagcacga ggacggccgg tttgaaatca tcacctggac cgacccgctg cgccgggcgg
    12601 ctgccgtgac ctcggtcgcg gcgcgggccg cctgatgcgc attctggttg cgaccgatgc
    12661 gtggcatcct caggtcaacg gggtggtgcg gacgctgacc aatatggcgg aggcggcaag
    12721 ggcgttcggc gtcgaaatca gctttcttac cccgcaatcg ttccgcacct tcgcgatgcc
    12781 gagctatccg gatctgcggc tggcgttgcc gtatcaggcc aagatagccc ggctgatcgc
    12841 cgaggcacgg cccgacagca ttcacatcgc aaccgaaggg ccgatcggcc tgttggtgcg
    12901 gcgctattgc cgcaaaaatc aattgacgtt caccacgagc tttcacaccc gctttccgga
    12961 atacatatcg gcgcgtctgc cgatccccga agcgtggata tggacggcgc tgcgcgcatt
    13021 ccatgggccg agccaggcgg tcatggcggc gacgccggcg ctggccggcg aactgcgggc
    13081 gcgtgggttt cgaaacgtcg tgttgtggcc gcgtggcgtt gacgccagcc agtttcatcc
    13141 gcgcgtcgcc gaccttggct tgccgcgccc ggtcttcctg tgcgtcggcc gcgttgcggt
    13201 ggaaaagaac ctcgaggcat ttctggacct cgatctgccc ggtaccaagg tgattgtcgg
    13261 cgatgggccg gcgcgcgcgg cgctgacccg aaaatatccg caagcggtgt ttctcggcgc
    13321 gcgtcagggc aaggaactgg cggaggccta tgcggcggcg gatgtcttcg tgttcccgag
    13381 caagaccgac acttttggcc tcgttctgct ggaggccctc gcgagcggcc tgccggttgc
    13441 cgcgtttccc gtgaccggtc ctcgcgacgt gatcggcgat gagccggtcg gcgcgttgaa
    13501 tgatgatctg cgaatagcct gcctgaccgc agtgacaatt tcgccacaag cctgcctggc
    13561 gttcgccgca aggcacacct gggaagcttc agcccgcgct ttcgtcgaaa atatcacgaa
    13621 tgtccgcgcc ggtgacccga aaagcgatgc gctacaattc gaggtgaagc gcccgcgttt
    13681 cgtcgcctga ccgcttgccc gcctgttggg gtccgcgata caagcgtcgg gatggcggac
    13741 ctttccccaa tcttgcctgt cagccctgcg gatatcgacg cggccgcgcg cgtgctggcg
    13801 ccgtacgcgg tgcgcacgcc gctgttgtcg gcggccgttc tcgacgagcg tgccggaacc
    13861 cgtgtctttc tgaaaccgga gatgctgcag cggaccggtt cgttcaagtt tcgcggcgcc
    13921 ttcaacaggc tgtcgtcgat tcccatggct gcgcgcggtg gtggcgtggt cgcgttctcg
    13981 tccggcaacc acgcccaggg cgtggcggcg gcggcgaaaa tcctgaacat gcaggcgacc
    14041 atcgtgatgc cggccgacgc gccgctctcc aagcgcgaac ggaccaaggc ctatggcgcc
    14101 gaggtggtgc tgtatgatcg cggccgcgag gatcgcgaag ccatcgccaa cggcattgcc
    14161 ggcaaacgcg gcgccacgct ggtgcgcccc tatgacgatc cgtttgtgat cgccgggcag
    14221 ggcacggtgg ggctggagat cgcggaggat atggcagcac ttggcctgac gcccgatatc
    14281 gtggtggcgc cggcatcggg cggcggcctg atcgcgggtg tcgcgaccgc ggtcaaggcg
    14341 cgctatccga aggccatcct gatgtcaggc gaaccggacg catttgacga tcatgcgcgc
    14401 tcgctgcgcg ccggcaaacg cgagccgcat cgcgccgaag gccgcaccat ctgcgacgcg
    14461 ctgatggcct cgattcccgg cgaaatgact ttcgccatca acagccggct gctcgcgcaa
    14521 gggatcaccg cttccgatgc cgaagtcgct gccgcggtcg gattcgcatt ccgcgagctg
    14581 aaactggtgg tggaaccggg tggcgcggtg ggcctcgcag cgcttctggc cggccgtatc
    14641 gatggccgcg gcaagaacgt cgtcatcgtg ctgtcgggcg gcaatgtcga tgcggatttg
    14701 ttcgcaaaat taatcagtta agcgagacat caggagaaga acgcgatctt ctcggcctgg
    14761 ctcatgcgcc ggatcggcgc cagcgggtcg gactgcgatg cattgggttt tcgcagcaaa
    14821 ccgctcgcga gaagggccga gccaagggag ggttcaaggg aaggttcaag ggaaggttca
    14881 ggcgaggctt gcagcgatgg ttgaagcgaa gtctgcagcg acgtcgacat cgcgggcacg
    14941 gccgtcggcc caggctgcgc aacgaggacg gggccggccg gcggcgaggc cgtgacgtca
    15001 atctcgtcat gggcggtatc ggcgggaaga tcgatatcag aaggatccgg cgccgccatt
    15061 tcaagcgcga ccatccggag cacggcgtcg tcctgggcat cgacgtccag ggtatcagag
    15121 gcgggcatat cgccggcttg ggcataggca gcctgggcat cggcggcttg agtatcaggg
    15181 gcgtaattgc cgagggcttg ggcgtcggcg gcctccgcgg tcatctccgt tgcaccgagc
    15241 gtttcgtgcg gcatcgccgg ggcgttcgtc gcggtgtcga tgtttgttgc ggctgccgcc
    15301 ggcaccatct ccaaatccgc ggcgggcagc tcgcctggcg gggtggggag gggcgagggc
    15361 cgggagacgg ctgtttcggc ggcctgcggg gatgccgtat cgctctcgcc caactccgcg
    15421 atctgtgcct cgatcagatc gaatgcagcg ctcaataccg cttttggatc ggtcgatatg
    15481 atttgcccgc aagcaccttc gatggcgttg acctgggaat cgagcaggtc gcagatccgg
    15541 ccgtcggtgc cgatttcccg ccatcgccag gcgatttccc tgatcacccg ggcgcccttg
    15601 cggaccggcg cgagggtttc ctccagcgcc agaccatcga tggcgccgga tgcggcggct
    15661 ctggcctcat cgaccgccct cctgatcacg accaatgcct cggcgagccc attgtcgggg
    15721 gccgcctgct tctgggcggc caggttttct tcgacccgcg ccaccgcatc gagcaccatg
    15781 cgggtgtcgg cattgcggtt gcgcttggca tactcgccga gaaaccagcg gccgcgcgcc
    15841 gtctccatga acgcttcgcg gatcgcgtca tagtcctgct cgctcggctg ggttgcgcga
    15901 gcggaaatcg gcgatagggc aaatgcgtcg tcggccatca taaactcatc acgcaaatca
    15961 ccgcgcgtta cgttaacgat catcatgata tcaagcgaat cgcaattgaa ttgatgccgc
    16021 ccgaatcaca aatccccatc gcatctcagg acacggccag gcgattcgca acacggctgg
    16081 cggtgtttta cggcacgatg ttcggcatga tgggcacgca tctgccgttt ttcacggttt
    16141 ggctgaaggc ggtcgggatc gacgcgttct ggatcggtgt catcacggca gtcccgccgg
    16201 tgacgcgttt cacggttctg ccctttgtga cggcgctcgc cgaaaggcgc catgcgctgc
    16261 gcggcgccat cgtggtgacc ggctttgcaa cagcattctg gttttacatt gtcggcaccc
    16321 agcatcagcc ggtcctggta tttcttgcct acgccgtcac ctgctgcctg tggacgccga
    16381 tggtgccgct gaccgatgcc tatgccttgc gaggcgtggc gcgctatggc ctcaattacg
    16441 gaccattgcg gctgtggggc tcggccgcgt tcgtggcagg agcgctggtc tgcggactgc
    16501 tggtcgatgt catcgccgcc aagaacctga tctgggttat cgcgtcagtg gccgcccttg
    16561 gcgccttcgt cggcctcggc cttcagccaa tgggcaagcc aaaatcagtt acgcatatcc
    16621 agcatggcgc cggcgccttg ctgcgcgatc gcggttttct cgccatcatc gtgacgtcgg
    16681 cgctgatcca gggcagccac gccgcctact acatctttgc ttccatcgcc tggcagcagg
    16741 cgggcctcgg cgggctgacc attgccgggc tttggtcatt gggggtgatc gccgagatcg
    16801 tggtgttcgc gctgtcgccg cgatttacgc tgccgccggt cttcctggtg gtgatcgccg
    16861 cattgagcgc ggtcgcgcgc tgggtgatca ccgcgcagca accgccgctg gcgatcctcg
    16921 ccaccgttca actggcgcat ggcctgagct tcggcctgac gcaggtcggc accatggggt
    16981 tgctggttcg ctatgtgccc ggtcatgtga tggcgcgcgg gcaaggttac ctcgcggcct
    17041 gcagcggcat cgtttcgagc agcgtgtcga ttttatccgg cgcgatctac gcccagtacg
    17101 ggcagggcgt ctattatgcg atggcggcga tggcgctgtc cggcgccgtc ctgatgtggc
    17161 tggcgcgaca ccggctggcg ggtcaccccc aaagcgccgc atccggcgga tagaccaggc
    17221 tgccgtcata gcgcagcccg ccatcgcggt cgcgcgccag caacaacggg ccatccagat
    17281 cgacgaagcg ggcctgttgc gtcagcagca tcgccggcgc catcgacagc gaggttgcga
    17341 ccatgcagcc gatcatgatg tcaaatccga gcgcgcgcgc cgcgtccgcc atcgccagcg
    17401 cctccgtaca cccgccggtc ttgtcgagct tgatgttgac ggcgtcgtag cgcccgcgca
    17461 gcccgtccag cgacgcacga tcatgcacgc tttcatcggc acagacggca ataggcctgc
    17521 gaatccgcgc cagcgcctcg tcccggccgg cgggcagcgg ttgctcaacc aaggtgacgc
    17581 cggcctcggc gcaaacggca agattttgtt cgagattttc ggccgtccat gcctcgttgg
    17641 cgtcgacgat gagttccgat tcgggagcca cctggcgcac cgccgcgata cgcctcccgt
    17701 cggcgtcgcc gccaagcttg atcttgagca gcggccggtg cgcggccttg gcggtggccg
    17761 ccgccatcgc ctccggcgac gccagcgaga tggtgtaggc cgtgatgcag gcctccgggg
    17821 cggggcgccc gagcaggttc cagacgcgct gaccgctgct cttggcctcg aggtccagca
    17881 acgcgcagtc cagcgcgttg cgggcagcgc atgcgggcat cgcggcctgc agggcccacc
    17941 ggtcgaggcc ccgggccaag ggttcctgca tcgacagcag ggcggccagg gtggcttccg
    18001 gcgtttcgcc atagcgcggg tagggggcgc attcgccgcg cccggtacgg ccgccacagt
    18061 tgagttccgc caccaccgtc accgcctcgg ttttggcgcc acggctgatg gtgaagctgc
    18121 ctgcgatcgg ccagcgctcg atcctgccgg caagctttgg gaatttggtg gaagtcattt
    18181 taaaatctgg caatttctga accggccggc ttgcccggcg caaccaagta tggctggtta
    18241 ggggtgcatt ccacaaggcg gctgttggaa ctttcccgtc catgaatagc ggagaatcag
    18301 cacaggagtt ggcatcgtga acggcgaccc gacattggag cggattgcac aaggcaacgg
    18361 actggcgctg tgcgctacgg gttcgtggac cgcccggttt gcgccggtgc ttgagcagat
    18421 ggtggcggac gccgaaaggt tgaccggaag ccggcccgac atcttcatcg atgtgtcgaa
    18481 ggtctccaaa ctcgacacct tcggggcctg gctgatcgaa cggcttcgcc gcagtctggc
    18541 ccatggcggc gtcgaggcca ggatcgccgg gctgtcggcg aactattcga gcctggtcga
    18601 cgaagtgcgc cggttgaaag cgaccgccgt ggtcgacacc acgtcgatga cgattaccgg
    18661 cgtgctggat caaatcggcc gcaacatggc cggcttcggc accacgttga tcggactgat
    18721 cgacatgctc ggcgccgttc tggccgcagt cgggaggatg gtgattcatc catccgggtt
    18781 ccgcctgact tcgaccgtgc atcatctcga gcaggtatgc tggcgggcgg tgccgatcgt
    18841 gctgctcatc acctttctga tcggctgcat catctcccag cagggcatct ttcatttccg
    18901 caaattcggt gccgatattt tcgtggtcga catgctgggc gtgctggtgc tgcgcgaaat
    18961 cggcgtgctg ctggtggcga tcatggtggc gggccgttcg ggcagcgcct ataccgccga
    19021 actcggctcg atgaaaatgc gcgaggagat cgacgcgctg cgcaccatgg ggttcgaccc
    19081 gatcgaggtt ctggttctgc cgcggatgct ggcgctgatc attgcgctgc cgattctggc
    19141 ctttctcggc gccatggcgg cgctggtggg cggcgggctg gtggcgaact tttatggggg
    19201 cgtcgatccc gaggcgttca tgcttcggct gcgcgacgcg atttcgatcg atcacttcat
    19261 tgtcggcatc gtcaaggcgc cggtgatggc ggcggtgatc gggatcgtcg cctgcgtcga
    19321 gggtctggcg gtcgagggca gcgccgagtc gctcgggcaa cacaccacct cctcggtggt
    19381 gcaggggatt ttcttcgtca tcgtgatgga tggcgtattt gcgatctttt tcgcctcgat
    19441 tggaatgtga cgatggcgcc tgaaattccc gacgccatca tccgggtgcg cgacatctcg
    19501 gtacggttcg gcgccaccaa ggttctggac gggctcaatc tcgacgtgaa gcgcggcgag
    19561 atcctgggct tcgtcggtcc gtccggagcc ggcaagtcgg tgctgacccg caccatcatc
    19621 ggcctcgttc ccaaggtcag cggccgtatc gaggtgttcg ggatcgatct cgacgccgcc
    19681 aatgccgccg cgcgacgcgg ggtcgagcgg cgctggggca tcctgttcca gcagggcgcc
    19741 ttgttttcct cgctgacggt gcggcagaat atccagttcc cggtgcgcga atatctcaac
    19801 gtctcgcagc gcctgctcga cgaaatcacg gtggccaagc tcggcatggt ggggttgcgc
    19861 cccgaagtcg ccggccgttt tccctcggaa ctgtccggcg gcatgatcaa gcgcgtggcg
    19921 ctcgcccgtg cgctggcgct cgaccccgaa ctggtgttcc tcgatgagcc gacctcgggc
    19981 ctcgacccca tcggcgccgg tgactttgac gaactggtcc ggaccctgca gcgcactttg
    20041 ggcctgaccg ttttcatggt aacccacgat ctcgacagcc tctacaccgc ttgcgaccgt
    20101 atcgccgtct tagggaacgg taagatcatt gccgcaggat cgatggccga catgcaggcc
    20161 tcagagcatc cctggttgag ggcgtatttc cacggcaagc gcgcccgcgc ggtcatgggc
    20221 tagttatacc ggagttggtg gaatggaaac gcgggcgaac ttcgtcctga tcggatcgtt
    20281 cacgctggca gtgattgccg cggcgttcgg ctttgtgctg tggttccagt ccctccacac
    20341 caccaaggcg cgcagcccgc tccgcgtcgt gttcgaaggt ccggcaacgg gtttgcgcaa
    20401 cggcggcagc gtgaatttca atggcatccg ggtcggagag gtgatctccg tgaagctgga
    20461 caacccgcgt cgagttgtcg cactcgcgat ggtcgagaac aacgccccga tccgcaagga
    20521 cacgctggtt gggctcgaat ttcagggact aaccggcgtc gcggcgatct cgctgaaggg
    20581 cggcgaggag gctgcacccg gtgtccccct cgacgaggat ggcgtgccgg tgctgaccgc
    20641 cgatccgaac gcgctgcagg acgtcaccga ggcgatccgc ggcaccctgc agaacatcaa
    20701 ccgggtggtc gccgacaacc aggagacggt caaggcctcg ctcaaaaacc tcgagacatt
    20761 caccgcggcg ctggcgcgca attccgagag gatcgacaac gtcatgctga aggtcgacgg
    20821 cgtcgtgggc aaggccgaca acctcatgct gggtctcaat acgctggccg gcggcaagga
    20881 gggcggcgaa ttgttccagg cggtgaaatc gatccgcgaa ctggccgaag actttgacaa
    20941 gcgatcgggc gccttgatga ccgacggccg ccgcacgctc ggcgacatca gccgcgcggt
    21001 gaacaatctc gaccgtaacc cgacccgcct gctgttcggc gccggcaaca gcaacgccgc
    21061 atcggcgccg ggtccgggtc ctacgcccgc ggcggcgcag gcgtccgagc ggaagcggca
    21121 atagggggag tatggcgggc gccggtattt cgcggagatg gcgtgtcaca agacctcttg
    21181 gctgcggggt cacagattca attttcaaac agcgccattg cagacgcacc ttcgcgatct
    21241 cgccgcgcct tgcgcgcgag ttttgagtga acgtccggcc cttctgagat cagagggcgc
    21301 agggaatgcc gggcgcccga tgcgcccgca gccgcgcgtg gtgtgctagt aaacacgcgc
    21361 gttagtcacc acggtcacac cggaaaacgc ccggcattcc ccgcgcaatg gttttaacgg
    21421 tttccttcgt gctctccccg gtgaccgggc ttgtttgcca ccgtcatctg cgaaaatgca
    21481 tccgcaaact tgacgccggc gtcgaggcgt caggaccaca cgacttcgcc gtccgcatca
    21541 gcgccattcg tcaaaggcgt atccgcgtcc accgcatccc gccccgcgtc cgtgacgatc
    21601 gcgagccgcc cctctgtggg acgggatggc aatcgatata gatgtgattt ggggtaggtg
    21661 agaaatggaa tctttttcaa aagggggctg gacagaccca tcgcctgacg gcccgtcggg
    21721 caaatcagtc gatctgtccg ccgcagctcg aaaccgttga tgacacgaaa ctcagcccca
    21781 atcgttgcaa atgcgctgct aacgtttttt ccgggttcgc tttgacatct cagttcgtga
    21841 ctggaatcga tcaggagtga aatctttagc ctcgacgaga tcgggctctt tccccgtcaa
    21901 tagctttgct gcgaacggct cggatgtgta tccctcaatt ggacagtcgc aagacaggag
    21961 ttggcctgta catattgggc aacgctcaac atcgcagccg ggaacatgca gttggccgcg
    22021 acgcacgccg cagtctttgc agttcgctcg catgtttagc cttgggacat actcggcgcc
    22081 gtagggaatg cgcttgatcg ttgcgttata agtcaaatgc ctcatcactt gagaattgcg
    22141 gatacgcgag ggccacgagc ttatcatctc agccccgcca aactttacct tttgccctcg
    22201 aaccttggtg cgattctttc ggcgcgccag ttcaccccga tcaagtcggc ttccttcgaa
    22261 cgctccaagc tttcgaagta gtagaccgca aatcgccatt ggatgagtct tgcgcacatc
    22321 tatgaaacca gtggttttgt tcagtcccgg cagttcaacg tcctcaatta ttaccgggag
    22381 gatatattct gatgaactga aaaacgcgcg ctcttgagct tgtcttaatt catgcttcgg
    22441 ccaaaccttg tctttgtagg ctttcgacac gaagactaga cagtatttgg ctttgtcgcg
    22501 gtaaactgca gacaaatgct gaaataagtc cttaccccat agctcatgag taacgaactc
    22561 atcgtaaaag actttaacat cgcaatccac gagctgacgc gcaattgatt ccactatccc
    22621 gcgatcttcg ccagcgaatg atagtgccac gtcatattga aataagggaa tgtagtcgtc
    22681 atcatttgcc atgtgctgac cgctttctag taaatggcat taccctgaca ggcgactttt
    22741 ttaatgtgcg gaagaagtag ggatgggtgg atttctcatc ataaccgcct atctgcaggt
    22801 tcagctttag caattcgttc ttcgcatact ccggtctacg aacgacgatg aaaccaccaa
    22861 ctcgttccat gaaggccatc atcatcttgg ttggaccatc ttcaactgga acgtgtccgc
    22921 tgccgtcgcg tgggtccgca tgcaggccgg cgctgccgac cgaaagataa atcacgaatc
    22981 tgcaaaaaac aaaaacggag gcatcgctgc ctccgttttt attcagcgat ccgcgtgaag
    23041 cctagcctag ccgtcccgcc gcgtgggcga gcagggtata gaccatgccg gtttcggaga
    23101 cgaggtggct gcgcagcgcc gcgggtccgc ggtcgtcgcg ggcgatctcg tcgagcaagc
    23161 gctcgaattc ggtgatgtag cggtcgaccg tctgcttgaa ggtgcggtcg gcgcgatatt
    23221 tgcggccgac ttcgtcgaag gccttttggc cggccggtgt gtagagccgc ttggtgaacg
    23281 ccttgctctc gccgcgctga tagcgatccc acatctcgga tgcgagattg cggtccatca
    23341 ggcggccgat gtcgagcgac agcgactcca gcgggttggc gcccactccc ggcgtctgcg
    23401 gcgcacgccc gcgcggggct tcgcggctgc tgccgggaac gtcggcgcgg ttcaacaggt
    23461 cggagagcca cccatcgcgt ccctgctcgg gattggccgg gctgaccggc ggcgcttcgg
    23521 tgcggcgcga agccggcgca ccgaggtccg gcggcggcaa gctcgatgcg ctgctggcgg
    23581 cttcgcgcgg gcgcggcggc gcggcccgca ccggcgccgg cgcctcgctg cggccggcgg
    23641 ccgcgacagc gtgcaccggc tcttcgtcgc gggctgcgct ggtgcggccg gcgctgacca
    23701 cgtccagccc gcggccgtga cgcgcgacga tccggttcag ttcggccagc gcctcgatct
    23761 ggtcgacgat gaccttgcgc atctgcgagg tgctctcggc ggcttcctgc ggcatttcca
    23821 gcacgccgcg gcgaagctcg ttgcgcgtgg cctcgagttc gttgtgcatt tccagcgcca
    23881 tctgcttcat gctggagacc atcgaggcga acttgtccgc cgactgcttg aacatcgcat
    23941 cggcttcgtg cgtggtctgc tgatacattt cgctcatggc gtcggtggtc agccggcgtt
    24001 cctcctcggc cgaggcgcgc accgactcga actgccggct gatcgccgca gaccccgcac
    24061 cggcggtttc ggcgaccacg cgtgcgatat cgcgcgcgcg cgactcggcg gcggccagcg
    24121 attcatccag caggccggtg aaccgcgaca accgatgatc gagatcggcg gttcgcagat
    24181 cgatcgtcgt caccagcgat tcaagctcgg ccttgcgctc ggcgacggag agggaggtgt
    24241 cgcggttgct ttgctcgacc aggccggcgg cttcgaccag cgccttgccg tggctgtcga
    24301 actggctgga aagcgagcca aggtcctcca gcgcccgcga ggtcttggtg ttgaagatcg
    24361 acaactggtc ttccagcgtc tgggtcgcga cgccgttgcg cgaggtgaca tcgttcatgg
    24421 cggagacgaa gtcggccacg cgcgtcacca gggcgcgctc gagcgaattg agattgtcgt
    24481 gggcgccggt cagcacctcc tgcagcagga tattgccttc gcgcaggcgc tcgaacaggg
    24541 cgacggtatc ggttcgcagg atcttgctgg tttcctgcat ctcggtcacg gccgcgatcg
    24601 acacctggcg cgactggtcg atcgccgttc gcgaagcctg ctcgagctct ttcagcgact
    24661 tgctcaccgc ggtggttgcg atctcgccgg cgctcgtgat cgagcgcgcg acgtcggacc
    24721 cgttggccgc catcgattgc gagaacgcca gtcccctggt ttcgatggat ttcagcgaat
    24781 ccgacgtgac gcggtccata tcgaccgtca gctggctggc ctttgcgccg atggcctcga
    24841 ccagcgatcc acgcttgcca tcgatcatct gcgcgagacg atcggtctgc tgctggacat
    24901 aggtgacgat ttcgtcggtc ttgcccgtca tggtcgagct gaaggtgccg ctggcggcga
    24961 gaaccgagcg ttcgacatcc gccgaggtcg acttgacctt cgaactgact tcgttggata
    25021 cggccaccag ggcgatctgc gcattgagcg cactggtctg gatattgtcg gtggtactgg
    25081 cggtgaccgc gccgagcgac cgctcgatgt ccgcggtgat cgccttgatc tggttcgcgg
    25141 cctcgcccga ggtcgccgtc agcgacaggt gtgcctcacg ggcactgccc agaattgaag
    25201 cggcggtgtc ggccgcgagc gacttgacgt ggctggcggc ttccgacgat gcggcgacca
    25261 gcgtgctctg cgcctcgcgc gcggcggcga ccacggaact ggcggttgcg gtgccggcag
    25321 ccgacaggct tcgttcgacg tcggcggcca gtgacttgac ctggctcgca gcatccgccg
    25381 aggccgagac cagcgtggtc tgggcctcgc gcgcgccggc gaccactgaa ctcgcggtcg
    25441 cggtgccggc cgccgacagg gtccgttcga cgtcggcgga caacgatttg atctggaccg
    25501 cggcctctcc ggaggccgtc gtcaatgtgg tttgcgcctc gcgggcactg ctcaaaatcg
    25561 agtttgcggc accgctgccg accgcggtga gggcgcgctc gacttccgcc gaggtcatct
    25621 tcaactgcgc gccgacgtcg gaggaaaccg tcagcagcga ctgctgcgcg gcgcgcgcgc
    25681 cggtctggat ggtttcgctg gtattgacca ccaggttcgt cagcgagcgt tccgcatcct
    25741 ccacatggga cttgatccca agggacaatt cctcggcgcg cgccatcaga tcatcgctgg
    25801 cctgccgtcc gctgctctcg atgcggcctg ccacggcttc gacgcgcgac cccagcagat
    25861 cctcgaactg cgagacccgc atatcgatat cggtggcaac cgcgccgacc ctgctttcga
    25921 tgccctgatg gatttcctga aaccgcgccg tgatggtgtt ggtcagctgg gcgctgcgac
    25981 cgtcgatggt ttcagtcaca ttggagatac gacggtcgac cgcctcgatc gcctgcgcgg
    26041 tgccgtcgct cagcgaagag gtgagcttgg tgaggcggga atcgatcgag tagatcgcct
    26101 gcgccgcgcc atcggtcagc gacgacgcca gcgtgccgag ccggttgtcg atggtttcgg
    26161 tcaccgactt cgcgcgcgta tcgaaggtct gttcgagcga tttgagatgg ccgccgacgg
    26221 actcgtcaaa ggatctgatc ttgctgtcga gcgaagtatc gaggttggcc acgcgggttt
    26281 cgagcgtggt ctcgaattgc agcagacgct ggtcgagcga ggcggtgatc tcgccgccat
    26341 tcgatgtcaa ccgggtgtcg aaggtatcga cataggtctt caggttttcc gagatatcct
    26401 gcgtgcgctg acccatgcgc tcgacgattt cgccgccaaa ggtcttgacg gtgcgatcga
    26461 attccgagat gtggcgggtg atgagcgccc cgagcgtgcc tgagtcgcgc gcgaattttt
    26521 cggccaactc gccgccctgg ttcttcacca attcgtcgaa agcgctcatc tgcagggaga
    26581 gcgtgtcgtg cgcggtttcg gtctggctca cgaccttggc caccagcgaa ttcacggtga
    26641 cgtccagcgc ttcgctggcc ttgtcgccgg aggtgaggat attcgtcgcc aggcggttgc
    26701 cggcttcgtc gatcttgttg acgagatcgc cgctgcgcag ctcgagttcc agcagcaggg
    26761 aatcgctgga attcttcaag gagtcatgga cctgttcggt ccgctcggaa attccgtcga
    26821 cgatggtgga ggagcggtgc tcgaattcgc tggtgatacg gtcgatccgc tcgttcagca
    26881 tctcgtggac gcggtcggcc agatcgacga actcgtcatg gacgtggccg gtcttgaaat
    26941 tgaggctggt ggtgaggcgc tcgctggcgt cgagcaccgc gcgggtggtc tcggcgctgg
    27001 cttcctcgag gcggtcgagc aggtcgccgc cgcgctcgcc gagcgcgagg atcatgttgt
    27061 cgccggcgtt gctcagcgcg ctcgtgatgt gggcgccgcg ttcttccagg gcgccggtga
    27121 tgctttttgc cacctcatcg acgcgggatg cgatcgcgtc cgagatcagc gcgatgtcgt
    27181 ggcgcaggtc gatctgcacg ccggagatcg cgctgcgaac ctgttcggcc tgtccgacca
    27241 gattgtcacg ctgatgggcg atatcctgca gcaaggcgcg gattcgcacc tcgttgtcgc
    27301 tgtaggcgcg ttcgagcgcc gagacttcat tggcaaccag ggtttcaagc tcgccggcgc
    27361 gcgcaatggc gcgttcgacg ccgtcgccca tggcggcgac ttcgcggcgg atcgcctgac
    27421 cgaccgtgac catcgagtcg cttgccgcgc cctcaggttc cgaaaaacgg attgcgacct
    27481 gcgccatcga ctgggcgatc atccgcaatt cctggccacg ccaggaaagg ctcgccagga
    27541 aatagaacag cagcaccggc gcgaagaaca tcgccgacag gccggccagc accaggatgc
    27601 cgctgccttg tccgaccgcg gcctgcagcg aagaaaggaa gctggccgtc agcagggctg
    27661 cgccgagaac ccagatcgcg gcgaaaatcg aggccagcgt ataaacattg cgggcgggcc
    27721 ggcccttctg aatggcctgc agaatttgtc cgatggtttc gcggtcgtcg ttggcggcgc
    27781 ggcggatcgg gcgttgtggc tcctcgctga tcggccgatc gtcgctcggc cgggtgtcga
    27841 aagggctctg gctgtaatcc ggcatcgggg aagcgatcgt cgacgtcgca tcgctgcgga
    27901 ccgaaccccg gttgccgtca atcggtgcgt cgctgatgtt gagggcttcc tgaatggcag
    27961 aaagcgcaac ttcggtgggg tctttggcct tctttggatt attcgccata ttcagtccca
    28021 cgcccctcgt tttaattcac gcgtccggag agcctggcga acgtccctcg caagctcgaa
    28081 accggtccca acgccccgcg aacatcctat tggctgggcg gtccgaatga aatggctgcg
    28141 attaatacaa tcttaatcat cgttaacagc gccgcagcgt aacgtcttgc cattccacag
    28201 catttttgcg cgagtcgacc aattgggcga ctcttcgcca aaaacaaggc aaacttgcgg
    28261 cgagccgggc gaaacctttc cttaaccact gatgtgtttg gctgccgcaa aaccccgccg
    28321 tcggatcgat ggggccgggg atttgggtta tcgccatggc gcttcatctg gaacggattg
    28381 actggatgcc atcgccgccg ctcgttcccg atgacggtcc gatcgatatc gagcacctcc
    28441 ggcgcatgac actcggcgat gccagcctcg agcacgaggt gctggcgatg ttctcagccc
    28501 aggctgtcgg cctgattggc gctctcgcca tgctgccaag tgacgccggc gcgctggcgc
    28561 atctgctgaa aggctcggcc cgcgcgattg gcgcctttca ggtggccgac gccgccggac
    28621 gtctggaaac cgcgatacgg accggcgacg atcggtcaga gccgttcgcc gagctcgatg
    28681 atgcggtcgc gcaggcgcgc acggccatcg atgcgatcct gcgccggtcc tgatctcagg
    28741 cctggtctta ggtcccacct tgggccccga aagcccttgc caatcaacgt cgcaggtttt
    28801 cgccccgacc gctggcgcgg agcggaccga cccgttatag gactgcccgg acctctctct
    28861 catccggcag cacgagcaat catggccaag attcactttg tcgaccatag tggcgaaacc
    28921 cgtaccattg acgtcgaaaa cggcgcgacc gtgatggaag ccgcgatccg caacgccatt
    28981 ccgggcatcg aagccgaatg cggcggcgcc tgcgcctgtg cgacgtgcca cgtctatgtc
    29041 gatgaggcct ggcgcgagaa ggtcggcgca ccgtccccga tggaagagga catgctggat
    29101 ttcggcttcg atgtgcggcc gaattcgcgg ctgtcgtgcc agatcaaggt gagcgacgcg
    29161 cttgacggtc tcgtggtgac gacgccggag cggcaggcct gaacaccacc acgggaagcg
    29221 aactccacga tcacctgcag catgctcgcg cgctagattc cgtccgcgcc gcggcgaaag
    29281 aagggtttta gcttctcgat gatctcggcc gtcagcggcg tcggtttgcg cgtggcctcg
    29341 tcgatcagca ccgtcaccga ttgcgccgat gccacgcatt taccttcaga aaataccacc
    29401 tgatcgaaag tcacggaggt gcgaccgagt ttcgcgacgc ctaatcccat ttcaatggtg
    29461 ccgggccagc gcagttcggc gcgaaaatgg atgtcgagcc gtaccatgat ccaggcgaga
    29521 ccctcgggca tcaacccgaa actgcggtcc ttcatcagcg tgacgcggcc ggtttcgaaa
    29581 taggtcgcat agaccgcgtt gttgacattg ctgattggga tcgagatcgg caaaacgaac
    29641 attgtccgac agccggtacg gaaaatcttc gagcagcggg gttgaatcga ggcgggccgg
    29701 tgcattcacg gggtcgatct ccgaagtttc atgccgttac agcgcagttg tcgaattgcg
    29761 gcaaggggtt gccggggtcg ctcgcaccat attatgcctt tcctcgctcc gccgccttgg
    29821 ctagacaggc gcagccgaaa gcgccgggcc cgacggccgg gtcaaccgaa aagaagtcga
    29881 gatgagcgaa gcgatcaaaa ccgatgtgct gattattggc gcgggtccct gcggactgtt
    29941 tgccgttttc gagttgggcc tgctcgatat caaggcgcat cttgtcgaca ttctcgacaa
    30001 gatcggcggc cagtgcgccg agctctatcc ggaaaagccg atctacgaca ttccggggat
    30061 tccattcgtc accggccagg gcctgaccga agcgctgctg cagcagatca agccgttcaa
    30121 tccaaccttt catctcaacg aaatggtgga gacgatcgag aaaatcggca atcccggctt
    30181 tcgtgtcacc accgatgccg gccaggtatt cgaatgcaag gtggtggtga tctccgccgg
    30241 cggcggatcg ttccagccga agcggccgcc ggtgccgggt atcgaggcct atgagggcac
    30301 ctcggtgttc tactcggtgc gcaagatgga gcagttccgc gacaaggatc tgctgatcgt
    30361 cggcggtggc gactccgcgc tggactggac gctcaatct
//
LOCUS       DATNOI010000541         3352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2368476,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000541 DATNOI010000000
VERSION     DATNOI010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2368476"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="VN968_22045"
     CDS             <1..135
                     /locus_tag="VN968_22045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL31952.1"
                     /translation="PQVLHTLAAHVSNSRTAMIPGATHPMFEQAPQKFCEIVLEFLGA
                     "
     gene            complement(172..981)
                     /locus_tag="VN968_22050"
     CDS             complement(172..981)
                     /locus_tag="VN968_22050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXL31953.1"
                     /translation="MSGDAAGFIGTIPQHYDQGLGPLIFVDYAADIARRVASGNPTRV
                     LETAAGTGIVTRKLRDALSNDARLTATDLNPPMIEVARTKFRAGEQVEFQPADATALP
                     FADGSFDAVMCQFGIMFFPDKAKSCSEVFRVLASGGRYVFSVWDSHRYNPFGRITHEV
                     VGAFFPADPPRFYNVPFSCHQIDPIKELLIAADFSDIGIAVVRLEKQIPDMALFARGL
                     VYGNPLIDQIRTRGGVEPGLIVDALVQALRHEFGADPGRMPLQAIVFSATK"
     gene            1111..1785
                     /locus_tag="VN968_22055"
     CDS             1111..1785
                     /locus_tag="VN968_22055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXL31954.1"
                     /translation="MIALDPTPNLIDQVYARILEAITDRTLPPGHRIRQSELAEKLGV
                     SRQPVSHALHLLHRQGLVAESGRKGFEVTQLDPRRIRQLYEVRGAIDALAARLAAGRV
                     KIDAAGRAHLEATLRAGRTTDKTTPLAALIALDVDFHRAIYRLAGNPAIEEMIAPQWP
                     HMRRSMATVLAELDYRQSAWAEHETIAAHILSGNAKAAESAALAHAMSAGRMTEERLK
                     APERAA"
     gene            1915..2715
                     /locus_tag="VN968_22060"
     CDS             1915..2715
                     /locus_tag="VN968_22060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXL31955.1"
                     /translation="MKLTQQQIDDFNREGWLFLPELFSPEEVALLAQEAVGIYDNDRP
                     EVWREKSGAPRTAFAAHLYNEAFGLLGAHPRMIDPVEQIFGEKVYMHQYKINAKSAFT
                     GDVWQWHQDYGTWKRDDGMPEPRAMNIAIFLDEVMPINGPLMLVPKSQTAGDLKAAHD
                     LETTSYPLWTLDEETVTRLVKEGGIVAPTGKAGGMLMFHGNLVHGSAGNITPYPRKIV
                     YLTLNAVSNYIRTPTRPEFIAHRDFTPIQTVDDDALLRLARAQRQAAE"
     gene            2900..>3352
                     /locus_tag="VN968_22065"
     CDS             2900..>3352
                     /locus_tag="VN968_22065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXL31956.1"
                     /translation="MNLHHLLNARRAAGKPVRVALIGAGKFGSMFLSQVPHTPGLEVP
                     VIVDLDPQRAREACRTVGWDDERIAATTFTAEAAKATSGHIEVIVEATGNPAVGIKHA
                     RAAIAAGKHIVMVNVEADVLAGPLLADEARKAGVVYSLAYGDQPALTAE"
BASE COUNT          679 a         1072 c         1056 g          545 t
ORIGIN      
        1 ccccaggtgc tgcacacgct ggcggcgcat gtttcgaatt cgaggaccgc gatgattccc
       61 ggcgccacgc atccgatgtt cgaacaggcg ccgcaaaagt tctgcgagat cgtgctggag
      121 tttctgggag cttgataacg agccctgctc gacgcgagag cgccgtcatc gtcacttggt
      181 tgcagagaac acaatggcct gcagcggcat gcggccggga tcggcaccga attcatgacg
      241 aagcgcttgt accagcgcgt cgacgatcag tccgggctcg acgccgccac gtgtccggat
      301 ctgatcgatc agcggattac cgtagaccag cccccgcgca aaaagcgcca tgtccggaat
      361 ctgtttttcc aatctcacca cggcaatgcc gatgtcgctg aagtcagccg cgatcaacaa
      421 ttccttgatc ggatcgatct gatggcagga gaacggcacg ttgtagaacc gcggcggatc
      481 ggcagggaaa aaagctccga cgacttcgtg ggtgatacgg ccgaacggat tgtaacggtg
      541 tgaatcccag acactgaaga catagcggcc gccggaagcg agtacgcgaa aaacctcgga
      601 acaggatttg gccttgtccg gaaagaacat gatgccgaac tgacacatga cggcgtcgaa
      661 gctgccatcc gcgaagggaa gcgccgtggc atcggcgggc tggaactcga cctgctcgcc
      721 ggctcgaaac ttggtgcgcg cgacctcgat catcggcgga ttgagatcag tcgcggtcag
      781 ccgcgcgtcg ttggataatg catcacgcag cttgcgtgtg acgatgccgg tacccgccgc
      841 cgtctccagc acccgcgttg gattgccgga ggcgacacgc cgcgcgatgt cggcggcata
      901 gtcgacgaag atcaggggtc ccagcccttg atcgtagtgc tgcgggatgg tgccgatgaa
      961 acctgctgca tcgccgctca tgacggatct ccaatcgccc tggcgcgagc atagcgcacc
     1021 cggtcgcaac tgcctgctct cttgaccccg gtgccaactc ttgctagttt ccgaattcgg
     1081 aattccgtat tccaaactgg aaagccgccc atgattgcgc tcgacccgac ccccaacctg
     1141 atcgatcagg tctatgcgcg gatcctcgag gcgatcacgg accgcacgct gccgccgggg
     1201 caccgcatcc ggcagagcga actcgcggaa aagctcgggg tgtcgcgcca gcccgtctcc
     1261 cacgcgctgc atctgctgca ccggcagggc ctggtcgccg aaagcggccg caagggcttt
     1321 gaagtcacgc agctcgatcc ccggcgcatt cgccagctct atgaggtacg cggcgcgatc
     1381 gacgccctgg ctgcaaggct cgcggccgga cgcgtcaaga ttgacgcggc ggggcgcgcg
     1441 catctggagg ccacgctgcg cgccgggcgc accaccgaca agacgacgcc gcttgcggcg
     1501 ctgattgcgc tcgacgtcga ttttcaccgc gccatctatc gcctcgcggg caatcccgcg
     1561 atcgaggaaa tgatcgcgcc gcaatggccg catatgcgcc gatccatggc gaccgtgctg
     1621 gccgaactgg actatcgcca aagcgcctgg gccgaacacg agaccatcgc cgcccatatt
     1681 ctgtccggca acgcaaaagc cgcggagagc gcggcgctgg cgcatgcgat gagcgcggga
     1741 cgaatgaccg aagagagact gaaggcgccg gagagagcag cgtagtaagt gagccgtcat
     1801 tccggggcgc gcgtgaacgc gaaccggaat ctcgatcaac aagttctgga ttccgggtcc
     1861 acgcgaagtc gcgtgtcccg gaatgacaag aataaaccaa aaggaggaac caccatgaaa
     1921 ctgacccagc aacagatcga cgatttcaac cgcgagggct ggctgttcct gcccgagttg
     1981 ttcagcccgg aagaagtagc cctgctggcg caagaggccg tgggcatcta cgataacgac
     2041 cgcccggagg tgtggcgcga aaagagcggc gcgccgcgca ccgcctttgc cgcgcatctc
     2101 tataacgagg cgttcggcct gctcggcgcg catccgcgca tgatcgatcc ggtcgagcag
     2161 atcttcggcg agaaggtcta catgcatcag tacaagatca acgccaaatc ggccttcact
     2221 ggcgacgtct ggcagtggca ccaggactac ggcacctgga aacgcgacga cggcatgccg
     2281 gagccgcggg ccatgaacat cgcgatcttc ctcgatgagg tgatgccgat caacgggccg
     2341 ctgatgctgg tgccgaagag ccagaccgcc ggcgatctga aggctgcgca cgaccttgag
     2401 accacctcct atccgttgtg gacgctggac gaggaaaccg tgacacgcct ggtgaaggag
     2461 ggcggcattg tcgcgccgac cggcaaggcc ggtggcatgt tgatgttcca cggcaatctg
     2521 gtgcacggct cggccggcaa catcacgccg tatccgcgca agatcgttta tctcaccctg
     2581 aatgcggtct cgaactacat ccgcacgccg acgcggccgg agttcatcgc gcatcgggac
     2641 tttacgccga tccagaccgt ggatgacgat gcgctgctcc ggctcgcccg cgcgcagcga
     2701 caggcggcgg agtaagtgta ggcacgagcc gacatcaatc agccgtcatt ccggggcgac
     2761 gccaacgggt ccggccgtta gccggcccga tgacaggctc cgcgtcgagc ccggaatcca
     2821 taaccaccgc ggggactatg gattccaggc ccgcgcataa gtgcgcgtcc cggaatgacg
     2881 agaattagga catacctcca tgaatcttca tcacctcctc aacgcccgcc gcgccgccgg
     2941 aaaacctgtc cgcgtcgcgc tgatcggcgc cggcaagttc ggctcgatgt tcctgtcgca
     3001 ggtgccgcat acgccggggc tcgaggtgcc cgtgatcgtc gatctcgatc cgcagcgcgc
     3061 ccgcgaggcc tgccgcaccg tcggctggga cgatgagcgg atcgcggcga ccacattcac
     3121 cgccgaggcg gcgaaagcga cttcaggcca tatcgaagtg atcgtggagg ccaccggcaa
     3181 ccccgcggtc ggcatcaagc atgcccgcgc cgcgattgcg gccggcaagc atatcgtcat
     3241 ggtcaatgtc gaggccgacg tgctggcggg gccgctgctc gccgacgagg cgcgcaaggc
     3301 cggcgtggtc tattcccttg catacgggga tcaaccggca ttaaccgccg aa
//
LOCUS       DATNOI010000542         4515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2387936,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000542 DATNOI010000000
VERSION     DATNOI010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2387936"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VN968_22070"
     CDS             complement(<1..426)
                     /locus_tag="VN968_22070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HXL31957.1"
                     /translation="MERSFKKEVQALTLGEGETFRGEGILAVTKALLQSGVSYVGGYQ
                     GAPVSHLLDVLVDAEDIIGDLGVQLETCTNEASAAAMLGASINYPLRGAVTWKSIVGT
                     NVAADALSNLASPGVVGGVMIILGEDYGEGASVIQERSYA"
     gene            complement(517..1491)
                     /locus_tag="VN968_22075"
     CDS             complement(517..1491)
                     /locus_tag="VN968_22075"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL31958.1"
                     /translation="MAAYVRNRLAIALAAAMLLAPAGPVAWSQATGPIKIVVPYTPGS
                     GPDILSRLMAEQIGRAQGPTVVVENRPGGGTVIGTEAVERAEPDGRTVLLVANSFVIN
                     PPLQRASYNPSTSFEPVCYLAATPMVLVVLGSSPYRTLNDLIAAARAKPGELAFASGG
                     PGSSLHIAIEVLKRAANLNITYVPYGGTAPAINALMGNHVAAVWADYPTVVSQLQSGA
                     LRGLVTTSHARIETLPDVPTLGETGVSKYEAEIFYGIVAPAKTPSDVLNRLSGWFSSA
                     LKAPDMKPKLAQQGLFPVGMCGEEFGAYLRRQVEEYSRVIREADITVK"
     gene            complement(1557..2006)
                     /locus_tag="VN968_22080"
     CDS             complement(1557..2006)
                     /locus_tag="VN968_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXL31959.1"
                     /translation="MVRPLLAAAFSLLIASPAALAADARQMEENKKIVAAFYDAAVNQ
                     KDFEKASQYLGARYTQHNPLAADGREGFKGFITFLKDKFPNNRSEIKRIFADGDYVIV
                     HVHAVREPGTRGNAIVDIFKLENGKVVEHWDVIQPIPEKAANDNGMF"
     gene            complement(2037..3335)
                     /locus_tag="VN968_22085"
     CDS             complement(2037..3335)
                     /locus_tag="VN968_22085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HXL31960.1"
                     /translation="MGTDAVAIIGFITLFALMLLRVPVGMAMGLVGVSGFAYLVNGDA
                     AFKIVGHTSMRTVTDYTFGVIPMFLLMGAFVTNSGMSRELFRAANGFLGHRRGGLGVA
                     TIAACGGFAAISGSSVATAATFSTVAYPEMRRYGYPQSFAAGVIAAGGTLGAMLPPST
                     VLAVYGIITEQDIGKLFIAGIVPGILAASMYMITVAVIVWARPKYLPAGPRTQWKERL
                     KGLRDIWATLLLFCFVIGGLYGGLFTPTEAGGMGAGGAFLIGVLRGRLTRLDIRRSLL
                     QATRTAAAVFTVLIGALLFGYFLTITQTPQKVTAFLTGLGLGRYGVLALIMVMYVVLG
                     CLMDALAMVILTVPIIFPVIKELGFDPIWFGVIIVMTVELGLIHPPVGMVVFVIKSVI
                     QDINFSTIFAGVLPFIATDLIRLVILIAFPAIALWLPSRM"
     gene            complement(3344..3889)
                     /locus_tag="VN968_22090"
     CDS             complement(3344..3889)
                     /locus_tag="VN968_22090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXL31961.1"
                     /translation="MAAAQSNPDVVVRRGRMDRFIDGIELLAAFFVGIVAADIFSSVL
                     LRYFFSVQIPDSYDFGRLLLGILIFWGIAATSYRGTHITVDLVYANVGPKLQRFIDVF
                     ATLVLLFVVSFQTWTLFDKVLSTRADNVLTFDLRLPTWPFFLVAWLGDVSAVLLIAVR
                     TYRLIFRPELMGGQYQIRPVE"
     gene            complement(4063..>4515)
                     /locus_tag="VN968_22095"
     CDS             complement(4063..>4515)
                     /locus_tag="VN968_22095"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HXL31962.1"
                     /translation="TEVRDVMEKGVAEAVTFPWGSVPLLGVDKVTKYHMDAPLHTVTF
                     QWLMSPRTYAAMSAAQKKVIDDHCTTEWAGKFADPWADFEHAGLAKVKAEPGHEVYTI
                     TDAQLDEWKKSAEPLEKQWTDNVRKAGGDPDAIMKELKQSLAQYKAAY"
BASE COUNT          836 a         1428 c         1501 g          750 t
ORIGIN      
        1 ggcgtaggag cgctcctgga tcacgctcgc gccctcgccg tagtcctcgc cgaggatgat
       61 catgacgccg ccgacgacgc cgggggaggc gaggttcgac agcgcgtcgg ccgccacgtt
      121 ggtgccgacg atcgatttcc acgtcaccgc gccgcgcagc ggatagttga tggaggcgcc
      181 gagcatggcc gcggccgaag cttcgttggt gcaggtttcg agctgcacgc cgagatcgcc
      241 gatgatgtcc tcggcatcga ccagaacgtc gagcaggtgc gacaccggcg cgccttgata
      301 gccgccgacg tagctcacgc cggactgcag cagagccttg gtcaccgcga ggatgccctc
      361 gccgcggaag gtctcgccct cgccgagcgt gagcgcctgc acctctttct tgaaggagcg
      421 ttccatcgcc tctaccttgg cgcgagggaa gctatatcgg cgccggcgcg aatgctacct
      481 gcggcggcgc cggtctgccg cggccacgcc gccgaactat ttgaccgtga tatccgcctc
      541 gcgaatgacg cggctgtatt cctcgacctg ccggcgcagg taagcgccga attcctcgcc
      601 gcacatcccg accggaaaga gtccctgctg ggcaagcttg ggtttcatgt cgggggcttt
      661 cagcgcgctg ctgaaccagc cggataaccg gttgagcacg tcggacggcg tcttcgccgg
      721 cgccacgata ccgtaaaaga tctctgcctc gtatttgctg acgccggtct cgcccagcgt
      781 cggcacatcc ggcagcgtct cgatgcgcgc gtgtgaggtg gtcacgagcc cgcgcagcgc
      841 gccggactgc agttgcgata ccaccgtggg atagtcggcc cagaccgcgg ccacgtgatt
      901 gcccatgagc gcgttgatcg ccggcgcggt gccgccgtag ggcacgtagg tgatgttgag
      961 gtttgccgcg cgcttgagca cctcgatggc gatgtgaagc gaggagccgg gtcccccgct
     1021 tgcgaacgcg agttcgccgg gcttggcgcg cgctgcggcg atcaaatcgt tcagcgtccg
     1081 gtagggcgac gagcccagca cgaccaggac catgggcgtc gccgcgagat aacagaccgg
     1141 ttcgaagctc gtcgatggat tgtagctcgc tcgttgcaag ggtgggttga tgacgaagga
     1201 attcgccacc agcagaaccg tgcggccgtc cggctcggcg cgctcgaccg cttcggttcc
     1261 gatcaccgtg ccgccgcccg ggcgattctc gaccaccacg gtcggtccct gcgcgcggcc
     1321 gatctgctcc gccatcaggc gagagagaat gtcggggccg ctgcccggcg tgtaggggac
     1381 gacgatcttg attggccccg tcgcctgcga ccatgcgacg gggcccgccg gcgccagcaa
     1441 catcgcggcc gcgagcgcga tcgccagtcg attgcgaaca tatgctgcca taagtcgtta
     1501 cccgcgcgga tggcctcact cgctgctacc gcggcagcat acgtcaacgg gcacggctag
     1561 aacatgccgt tgtcgttggc ggctttctcc gggatcggct ggatcacgtc ccaatgctcg
     1621 accaccttgc cgttctcgag cttgaaaata tcgacgatgg cgttgccgcg cgtgcccggc
     1681 tcgcgcacgg catggacatg gacgatgacg taatcgccgt cggcgaagat gcgcttgatc
     1741 tcgctgcggt tgttcgggaa cttgtccttc aagaaggtga tgaagccctt gaagccttcc
     1801 cggccgtcgg cggccaacgg attgtgctgg gtatagcgag cgccgagata ttgcgaggct
     1861 ttctcgaagt ccttctggtt gaccgcggcg tcgtagaagg cggcgacgat cttcttgttc
     1921 tcctccattt gcctggcgtc ggcagcaagc gcggcggggc tcgcgatcaa cagcgaaaag
     1981 gcggcggcga gtagcggacg aaccatctca acctcctgtg tcgaagcggt gcaaactcac
     2041 atgcgcgacg ggagccacaa cgcgatggcc ggaaaggcga tcagaatcac cagccgaatg
     2101 agatcggtcg cgatgaaagg cagcacgccg gcgaagatgg tcgagaagtt tatgtcttgg
     2161 atcacgcttt ttatcacgaa gaccaccatt ccgaccggcg gatggatgag ccccagctcc
     2221 acggtcatca cgatgatgac gccgaaccag atcgggtcga aaccgagttc cttgatcacg
     2281 ggaaagatga tcgggacggt caggatcacc atggcaagcg catccatcag gcatcccagc
     2341 accacgtaca tgaccatgat cagcgccagc accccgtaac ggccgagccc gagcccggtg
     2401 aggaatgcgg tcactttctg cggcgtctgg gtgatggtga gaaaatagcc gaacagcagt
     2461 gcgccgatca gcaccgtgaa caccgcggcg gcggtgcgcg tcgcctgcag caacgagcgg
     2521 cggatgtcga ggcgggtcag gcggccgcgc agcacgccga tcaaaaacgc gccgcccgcg
     2581 cccatgccgc cggcctccgt cggcgtgaac agaccgccgt agaggccgcc gatgacgaag
     2641 cagaaaagca gcagtgtcgc ccagatgtcg cgcaggcctt tgagccgttc cttccactgg
     2701 gtgcgcggac cagccggcaa atatttgggc cgcgcccaga cgatgacggc gaccgtaatc
     2761 atgtacatcg aggcggcgag gatgccggga acgatcccgg cgatgaacag cttgccgatg
     2821 tcctgctcgg tgatgatgcc gtagacggcg agaaccgttg atggcggcag catcgcgccg
     2881 agcgttccgc cggcggcgat gacgccggcg gcaaacgact gcggatagcc gtagcgccgc
     2941 atttccggat aggcgacggt ggaaaaagtg gctgcggttg cgaccgagga accggagatc
     3001 gcggcaaagc cgccgcaggc tgcgatcgtc gcgacgccga gtccgccgcg gcgatggccg
     3061 agaaagccgt tcgccgcgcg gaacagctcg cggctcatgc cggaattggt gacgaacgct
     3121 cccatgagaa ggaacatcgg gatgacgccg aaggtgtaat cggtcaccgt gcgcatggat
     3181 gtgtgaccga cgatcttgaa ggcagcatcc ccgttgacga gataggcaaa gcccgagacg
     3241 ccgaccaatc ccatcgccat gcccaccggc acgcgcagca gcatcagtgc gaacagcgtg
     3301 atgaagccga tgatggcgac cgcatccgtg cccatcgcca cgctcactcg acgggcctga
     3361 tctggtactg cccgcccatc aattccggcc ggaagatcag ccggtaggtg cgcacggcga
     3421 tcagcagcac cgcggacacg tcgccgagcc aggccaccag gaaaaacggc caggtcggca
     3481 accgcagatc gaaggtcaat acgttgtcgg cccgcgtgct gaggaccttg tcgaacaagg
     3541 tccaggtctg gaaggagacg acgaacagca ggaccagcgt cgcgaacacg tcgatgaagc
     3601 gctgcagttt cggcccgaca ttggcgtaga cgaggtcgac cgtgatgtga gtgccgcggt
     3661 acgaggtggc ggcgatgccc cagaagatca ggatgcccag gaggagccgt ccgaagtcgt
     3721 agctgtcggg gatctggacg ctgaagaagt atcgcagcag gaccgagctg aagatgtcgg
     3781 cggcgacgat gccgacgaag aacgcggcca gcagctcgat cccgtcgatg aagcggtcca
     3841 tgcggccacg cctgaccacg acgtccgggt tcgattgggc cgcggccatg acgggatcag
     3901 ccattcctca aatcgacgtg cgcgccggtc agcatgcgcg agagcgggcg gcgactgcaa
     3961 gcgccgctcg ggcgggtggg cgtctccgca agcagcggcg aggcgcccgc cgccgttttc
     4021 atcccgcgac ggcttgagcg agcgcggtgg ggaagctgcc ggtcagtacg ccgccttgta
     4081 ttgggcgagc gactgcttga gctccttcat gatggcatcc ggatcgccgc cggccttgcg
     4141 gacgttgtcg gtccactgct tctccagcgg ttcggccgac ttcttccatt cgtcgagctg
     4201 cgcgtcggtg atcgtgtaga cctcatgccc aggctcggct ttgaccttgg ccaggcccgc
     4261 gtgctcgaaa tccgcccacg gatcggcgaa ttttcctgcc cactcggtgg tgcaatggtc
     4321 gtcgatgacc ttcttctgcg cggccgacat ggccgcataa gtgcgcgggc tcatcagcca
     4381 ctggaacgtg acggtgtgga gcggcgcgtc catgtgatat ttcgtgacct tgtcgacgcc
     4441 cagcagcggc accgagcccc aggggaaggt gaccgcctcc gccacgccct tctccatcac
     4501 gtcgcgcacc tcggt
//
LOCUS       DATNOI010000543         3534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1114159,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000543 DATNOI010000000
VERSION     DATNOI010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114159"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1635)
                     /locus_tag="VN968_22100"
     CDS             complement(256..1635)
                     /locus_tag="VN968_22100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HXL31963.1"
                     /translation="MSPADHTIKSRVAAISVFASAGMAAAKFVVGVAIGSLALISEAL
                     HSSVDVVATVITWLVVRVSDSPPDEEHQYGHGKLESLSALGVIAMLYVLSGGILVASW
                     GRLREGVPLPTISAIPFVVLLIDIAVNFWRARALQRTARDTKSQALAADALHFASDVL
                     GSIAVIIGLALAGLGYWWGDAAAAIVVAIMIALLGLRLARSTVETLLDRAPEGASEKA
                     RMAIRTVPGVVGVERLRVRMVGPTYFVDAVVQVPRTFPIDRVDDIKRKAQQAVSAALG
                     DADLTFTTVPVARNNESVRERIMVIARNSGLAVHHVTVHDLGGKLTVSIDLEVDGGMP
                     LTAAHAIAHELERNIREEFGEDVEVDTHIEPLEPELPVGTDAAPERVETIKAALSRFA
                     ANGAIHDIHSVRVRNTDAGEIVNFHCRAAPSMSVIKVHENVDEIERALRRAFPTVKRV
                     ISHAEPPDA"
     gene            complement(1753..2721)
                     /locus_tag="VN968_22105"
     CDS             complement(1753..2721)
                     /locus_tag="VN968_22105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXL31964.1"
                     /translation="MKAYVYGANGAAITDVAKPAPKGTQVLVRVRACGLNRADLGMTK
                     GHAHGSAGGAGTVLGMEWAGEVAEVGPQARGVKPGDKVMGSGGAAFAEYTLADHGRLF
                     RTPSNMNFEEAATLPVALATMHNAVVTNGALQVGQSVLIQGASSGVGLMALQIAKLKG
                     ARLVIGSSTDAARRGRLKDFGADLAVDSSDPGWVEQVLKATDGEGVDLIVDQVSGKVA
                     NQNLAATKIKGRIVNVGRLGGTRAEFNFDLHAARRITYIGVTFRTRSIEEIREIFDEV
                     RKDIWPAVEQRKVQLPIDKVFAFDDIGKAFEHMEANRHLGKIVVTL"
     gene            complement(2758..>3534)
                     /locus_tag="VN968_22110"
     CDS             complement(2758..>3534)
                     /locus_tag="VN968_22110"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HXL31965.1"
                     /translation="ILPSIKPPPYAEYRQAIIECGIKIVETAGNKPQEHVTEFKKHGI
                     VVVHKCTSVRHALSAERMGVDAISIDGFECAGHPGEDDTPGLILIPAAADKVKIPLIA
                     SGGFGDGRGLVAALALGAEGINMGTRFMCTKESPIHQLIKEKIVANDERETELIFRTM
                     RNTSRVAKNAVSTRVVAMEKEGATFEQVRDLVAGARGKMVYATGNADEGIWSAGQVQG
                     LIHDIPSCAELVSRIMREAEAIIRSRLEGMMSGARRQAAE"
BASE COUNT          622 a         1133 c         1117 g          662 t
ORIGIN      
        1 cccttgatcg aatcggattg gacgcacgcg ctcgccgcgt gtgcgcgcgc catgcctttg
       61 gccccctcgg aaatcttttg cacccggttc ggaagtctcc cagccgcttc cgaatcacag
      121 cgatttgaat ccagttttgc caagctgtcg agagtcaacg attcgttaat gcaaaaaaat
      181 tttgtccacg cagaatcgag gcccgcaaaa cacgggcaaa gagagtccga aaggagaacg
      241 cgcggccgga ttccgctacg cgtccggcgg ctcggcgtgg ctgatgaccc gtttgaccgt
      301 cgggaatgcg cggcgcaacg cacgctcgat ctcgtcgacg ttctcgtgaa ccttgatcac
      361 gctcatcgac ggcgcggcgc ggcagtggaa gttgacgatt tcgccagcgt cggtattgcg
      421 cacccgcacg ctatggatgt catggatcgc gccattggcg gcgaagcgcg atagtgcggc
      481 cttgatggtt tcgacgcgct ccggcgcggc atcggtaccg acaggcaatt ccggttccag
      541 cggctcgatg tgggtatcga cctcgacgtc ctcgccgaat tcctcgcgga tgttccgctc
      601 cagttcatgg gcgatggcgt gggctgcggt gagcggcata ccgccgtcga cctcgagatc
      661 gatgctgacg gtcaacttgc cgccgagatc gtgcacggtg acgtggtgga cggcgagacc
      721 ggaattgcgg gcgatcacca tgatacgctc ccgcacactc tcgttgttac gggcgaccgg
      781 cacggtagtg aaggtgaggt cggcatcgcc aagcgcggcg ctaaccgcct gctgcgcctt
      841 gcgcttgatg tcgtcgacgc ggtcgatcgg aaaagtgcgc ggcacctgaa ccacggcatc
      901 gacgaagtat gtgggcccga ccatccggac ccgcaaccgt tcgacgccga ccacgccggg
      961 caccgtcctg atcgccatcc tggctttctc cgacgcgcct tccggcgcgc ggtcgagcag
     1021 ggtttcgacg gtggagcgcg ccagccgcag acccaataac gcgatcatga tcgcgaccac
     1081 gatcgcagcg gcggcgtctc cccaccagta gcccagtccc gccagcgcca gcccgatgat
     1141 gacggcgatc gaaccgagca cgtcggaggc gaaatgcaag gcatcggcgg cgagcgcctg
     1201 gcttttggta tcgcgcgcgg tgcgctgcag cgcgcgggca cgccagaaat tcaccgcaat
     1261 gtcgatcaag agcacgacaa acggaatggc cgagatcgtc ggcagcggca cgccttcgcg
     1321 caggcggccc caagatgcga ccagaatacc gcccgagagc acatagagca tcgcgatgac
     1381 cccgagcgcc gacaggcttt ccagcttgcc gtggccgtac tgatgctcct catccggcgg
     1441 gctgtcggag acccgcacca ccagccaggt gatgacggtc gcgaccacat caactgagga
     1501 gtgcagcgcc tcggagatca gcgcgagact gccgatggcg acaccgacca cgaatttggc
     1561 agcggccatg ccggcgctgg caaataccga gatcgccgca acgcgggatt tgatggtgtg
     1621 atcggcaggg ctcatgggcg cgggtttagc agcccatcag ttaagattaa agcgccgatc
     1681 aattgcgcta tcgctactca gtcgcagcaa cgattgttgc gaattgttcc tactctaaca
     1741 gggcagcgag cactacagcg tcacaacgat cttgccgaga tgccggttgg cttccatgtg
     1801 ctcgaacgct tttccgatat cgtcgaaggc aaacaccttg tcaatcggca gttgcacttt
     1861 gcgctgctcc accgccggcc agatgtcctt gcggacttcg tcaaagatct cgcggatttc
     1921 ctcgatcgaa cgggtacgga aggtaacgcc gatataggtg atccggcgcg cggcgtgcaa
     1981 atcgaaattg aattcggcgc gggtgccgcc gagcctgccg acattgacga tgcggccctt
     2041 gatcttggtg gccgcgagat tctggttggc aaccttgcca gaaacctgat cgacgatcag
     2101 gtcgacgcct tcgccgtcgg ttgctttcag cacctgctcg acccagccgg gatcgctgga
     2161 atccaccgcc agatcggcgc cgaaatcctt cagccgcccg cggcgtgccg catccgttga
     2221 agagccgatc acaagcctgg cgcccttgag ctttgcaatc tgcagcgcca tcaggccgac
     2281 gccggagctc gcgccttgaa tcaaaacgga ttggcccacc tgcagcgcgc cgttggtcac
     2341 gaccgcattg tgcatggtgg cgagcgcgac ggggagggtg gccgcttcct cgaagttcat
     2401 gttcgagggc gtgcgaaaca gccggccgtg gtcggccagt gtatattccg cgaacgccgc
     2461 gccgccggag cccatcacct tgtcgccggg tttgacgccc ctggcctgcg gcccgacttc
     2521 ggcgacttcg ccggcccatt ccatcccgag cacggtgccg gcgccgccgg cgctgccatg
     2581 ggcatggccc ttggtcatgc cgaggtcggc gcggttgagg ccgcaggcgc ggacgcgaac
     2641 cagcacctgc gttcccttcg gcgcgggctt tgccacgtcg gtgatggcag cgccattggc
     2701 gccgtagaca taggctttca tgaaatcatt ctcgcttgcc cggttacacg aagctgctca
     2761 ttcagcggcc tgacggcgcg cgcccgacat catgccctcg agccggctcc ggatgatcgc
     2821 ctccgcctcg cgcatgatgc gcgacaccag ctccgcgcag gaggggatgt cgtggatcaa
     2881 tccctgcacc tgtcctgcgg accagatgcc ctcatcggcg ttgccggtgg catagaccat
     2941 cttgccgcgc gcgccggcca cgagatcgcg aacctgttcg aaggtcgcgc cttccttctc
     3001 catggcgacg accctggtgc tgaccgcatt tttggcgaca cgcgaggtgt tgcgcatggt
     3061 gcggaagatc agttcggtct cgcgttcgtc gttggcgacg attttttcct tgatcagctg
     3121 gtggatcggg ctctctttgg tgcacatgaa gcgggtgccc atgttgatgc cttcggcgcc
     3181 gagcgccagc gcggccacca ggccgcggcc gtcgccgaac ccgcccgagg cgatcagggg
     3241 aattttcacc ttgtcggccg ccgccggaat caggatcagg ccgggggtgt cgtcctcgcc
     3301 gggatggccc gcgcactcaa agccgtcgat cgagatcgcg tcgaccccca tgcgctccgc
     3361 cgacagcgcg tggcggacgc tggtgcattt gtgcacgacc acgatgccgt gcttcttgaa
     3421 ttcggtgacg tgttcctgcg gcttgttgcc ggccgtctcg acgatcttga tgccgcattc
     3481 gatgatggct tggcgatatt ccgcgtaggg cggcggcttg atcgagggaa ggat
//
LOCUS       DATNOI010000544         3944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2611289,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000544 DATNOI010000000
VERSION     DATNOI010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2611289"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VN968_22115"
     CDS             complement(<1..286)
                     /locus_tag="VN968_22115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015242302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXL31966.1"
                     /translation="MPCCSPFCSRVPRTPLLWVIREELKLTGTKFGCGTGLCGACMVH
                     IDGRREFSCQVQVSEVAGRAVTTIEGLSPDSSHPVQKAWLAERVPQCGYCQ"
     gene            582..1037
                     /locus_tag="VN968_22120"
     CDS             582..1037
                     /locus_tag="VN968_22120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017957178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL31967.1"
                     /translation="MTTNFTVNGRAASTDAPPVTPLLWVIREELKLTGTKFGCGTGLC
                     GACMVHIDGKRVFSCQTQVSEMAGKSVTTIEGLSADSSHPLQKAWLAEQVPQCGYCQS
                     GQIMSAADLLKQNPKPTRAQIVAHMSTNICRCGTYQRIVRAVERAAREA"
     gene            1098..2668
                     /locus_tag="VN968_22125"
                     /pseudo
     CDS             1098..2668
                     /locus_tag="VN968_22125"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
     gene            2816..3259
                     /locus_tag="VN968_22130"
     CDS             2816..3259
                     /locus_tag="VN968_22130"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL31968.1"
                     /translation="MGFAFLNYSDSLVAGAAEVSLERSTGKVTLHNFWCALDCGIAVQ
                     PDNVTAQTESSIVYGLGLALMERISIKDGAVEQSNFYDYLVPRISDVPLMHIEVISTD
                     NPPTGAGQMATPLVTPAIGNAIAQLTGTRLRHAPFTPERVKKALA"
BASE COUNT          738 a         1227 c         1277 g          702 t
ORIGIN      
        1 actggcaata gccgcattgc ggcacccttt cggcgagcca ggccttctgc accggatgcg
       61 aggaatccgg cgataggccc tcgatcgtcg tcaccgcgcg gccggcgacc tccgagacct
      121 ggacttggca ggagaactcc cgcctgccgt cgatgtgcac catgcaggct ccgcagagcc
      181 cggtgccaca gccgaacttg gtgcccgtga gcttgagctc ttcgcggatc acccaaagga
      241 gcggcgtgcg cggcactcga gagcaaaatg gcgagcaaca cggcagagat acggcgcatc
      301 ggtgcgcgaa agcaggggat gtgaaaggga cgaccgagtg ttctcgataa tccaagatcg
      361 cgtaaagctt tgtcgcccgg ccgcaggttt caaacgcaac gcgcgcgcag gtcggaaatt
      421 gcgtctgtac ccctaccgcg tgcattggtt gatggtgtac cgtgatcgca gagccgcctg
      481 cgagacctgt cctgtttttt cagccgcagc ctcagctttt caatttgcgg ccattgacag
      541 ctctggtccc gcatccgtcg cctatctgta aggaggaaac gatgaccacc aacttcactg
      601 tgaacggtag ggcggcgtcg accgatgcgc cgccggtaac gccgttgttg tgggtgatcc
      661 gggaggagct gaagctcact gggaccaagt tcggttgcgg aacgggtctg tgcggtgcct
      721 gcatggtaca tatcgacggc aagcgtgtgt tctcgtgcca gacccaggtc tcggagatgg
      781 caggcaagtc cgtgaccacg atcgaaggcc tgtcggcgga ttcatcgcac cctctacaga
      841 aagcgtggct cgccgagcag gtgccgcaat gcggctattg ccagtccggg caaatcatga
      901 gcgcagccga tctgctcaag cagaacccaa agcccacccg cgcgcagatc gtcgcacata
      961 tgagcacgaa tatctgccgc tgcggaacct atcagcgcat cgtgcgcgcg gtcgagcgcg
     1021 ccgcgcggga ggcctgagcc atgacaacga atgtcaccgc gacgtccaac aatacttccg
     1081 tctcccgccg ccaggtgatg atcggcgcgg cgggactgtc atttgccttt gtgctcggcg
     1141 ccccgctcgc ccgtgctgca accttggcca ctgagcggac gggcaaagcg ctctcacctt
     1201 gggtgagcat cgcccccgat ggctccatca ccatcatgtc ggcggcgacc gagatggggc
     1261 aggggtcgat gacatcactg cctttgatcc tcgccgagga tctcgacgcg gactggtcga
     1321 aggtgcaggt cgtcgccgcg ccaccgatcg atgcgatcta cggcaatccc gggttcggcg
     1381 gcatgatgta caccgccggc agcaatgcgg tgcgcagcta ttacttatcc ctgcgcacgt
     1441 tcggcgcgca ggtgcggcgc gtgctgctcg acaacgcggc gaacaagctc ggcgtagcgg
     1501 tggacgagct gacgaccgag ccgagcgtcg tcgtccacgc caagtccggc cggcgactga
     1561 gttacggtga gatcgcggca ttcgcgcagg tgcccgacaa gccgcccgag atcaagcccg
     1621 agcaacttaa gaagccgagt cagttccggc tgatcggcaa ggatgtcatg cgggtcgacg
     1681 tcccgggcaa ggtgaacggt accgcccgtt atgctatcga cgtgcaggtg ccgggcatgc
     1741 tctatggcgc cgtgttgcgc gcgccggtcg agggtgcggc gcccgacaag accgacgaat
     1801 ccaaggccgc agccgtggcg ggcgtggtca agatgatccg gatgccttac ggcgtcggca
     1861 tcgtcgccga gacgccgtgg gcggctttcc aggcgcggga agcgttgagc cattccacca
     1921 cctggacgaa gtcaggaaag gcgtggggct tcgacagcga gaaaggactc gaggacttcg
     1981 ccgccgcggc gcgtgatccg agcgccgaag caacggagtg gtcgaagtcc ggcgatccgc
     2041 gcgatgcgct gactcgggct gccagcacgt tcgacgccga gtaccgctgc gattacgcgt
     2101 accatgcgca gatggagccg ttgaacgcgg tcgctgctgt gtcgccggcg ggcgactcgg
     2161 ccgagatctg gtgcggcacg cagagtcaga ccacagccca ggaggcaagc gcgaaggcgt
     2221 tgggattgcc cgcgagaagg tcaagctcaa ttacctgctg atgggcggcg gcttcggccg
     2281 ccgcgggccg cgcgatgccg acttcgtcgt cgacgccgtg cttttggcga aggaggcggg
     2341 ccgcccggtc aaagtcatgt ggacgcgcga ggacgacgtt cacaacggcc gcttccgccc
     2401 gctctcagcc cattatctac gggccgggtt cgacggctcg ggcaagctgg cggtctatca
     2461 tcatcggctt gtaggcgatc gagtgacgcc gtccttcgat ccggtgcgct atgaatcgcg
     2521 cggccgcaag gacttcatct tgatggccgg cgccgatttc ggcagctacg acgtcccgca
     2581 tcaattggtc gagcagttgt accgggacac gggcgtgcgc accgccccgt tgcgcggcat
     2641 cagctggact gcaaacaagt ttgcggtcga gacgtttgtc gacgagatcg ccgtaaagcg
     2701 agggatcgat ccggtcgctt accggctcga attgatcaaa gacccgcgcg cacgaagggt
     2761 cacagagacc gttgcccgga tggcggactg gggcaagaag cgcgacgggc gcggaatggg
     2821 atttgcgttc ctcaactatt ccgattcgct tgtcgccggc gctgccgaag tatcgctcga
     2881 acgctccacc ggcaaggtca cgttgcacaa cttctggtgc gcgctcgact gcggcatcgc
     2941 ggtgcaaccg gataacgtga cggcgcagac cgagagcagc atcgtctacg ggcttggtct
     3001 cgccctgatg gagcgcatct cgatcaagga tggcgcagtc gagcagtcga acttctacga
     3061 ctatctcgtg ccgcgcatca gcgacgtgcc gctgatgcac atcgaggtga tatcgaccga
     3121 caacccgccg accggggccg ggcagatggc taccccattg gtgacgcctg ctatcggcaa
     3181 cgcgattgcc cagctcaccg ggacgcgcct gcgtcacgcc ccgttcacgc ccgagcgcgt
     3241 caagaaggca ctggcgtaat ccaagcgccc ggcgcacctc ggggggcgtc ttgcccccgg
     3301 gtgcggcgct cgcattcttt gcaaattcgc cagcgcagcg cgtagggcgc cgcaatgcgc
     3361 tccgcctatg gcgccttacc cagctacgcg caattgccat cgcgagcaac ggcttgcgcg
     3421 ggctagatgt ttagcgcttg tcggcttgcg aacaaaacgg cacctcctgc gtttcttata
     3481 tggaacagga gcccagcaag ccctaacgca aggggcctga tcgtgtcgga cctagctcga
     3541 accaagcgcc gcgaccgaac tgtatcttgc ccacgatgtg gaaccactat ggaggaagtg
     3601 gtcagcatcg ccctgagctt aggtgaaccc ggactgagcg gctatgaatg cccaaattgc
     3661 gtttacgtga ccagcgagtt ggtgcggccc actgacgagt aacgtagggc gccatggccg
     3721 aagcggtatt gcgccattca tacctcggcg acttcaccct ccgcggtgat ttgtcagcct
     3781 ttcctgacgc gtgacacgac gatagcggta gctggttccg cagtgacagc ccgtcctgct
     3841 cgcaatcgcg ttgatacggc cgggatggga acacggcagg taagcttttg tttgccaacc
     3901 gaagctgaat tggtggccgg ctgctctttg ccctgatcta accc
//
LOCUS       DATNOI010000545         4248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1352913,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000545 DATNOI010000000
VERSION     DATNOI010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352913"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..877
                     /locus_tag="VN968_22135"
     CDS             2..877
                     /locus_tag="VN968_22135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HXL31969.1"
                     /translation="MLGNEKGGSGKSTTALHIAVALLKAGQRVATIDLDCRQQSFTRY
                     INNRRTWARRTGLDLQLPVHCCIKLGETMQIADNESSEFQQFMDAVSAVERTFDFIVI
                     DTPGSDSYLMRLAHSMADTLVTPINDSFLDFDVLGTVDPATYAVTGESHYAEMVRDTR
                     RKRRQLDGVSTDWIVVRNRLSMLGSRNKQLVADGLKDLSFRLGFRSIDGFAERVVYRE
                     FFPRGLTALDDLDETTLGTRPSMGHVTAREEVTSLLRQLKLPLDERGRRRAANRAEWF
                     MQVDKPLEVHDILGV"
     gene            1178..2032
                     /locus_tag="VN968_22140"
     CDS             1178..2032
                     /locus_tag="VN968_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL31970.1"
                     /translation="MKRGIAILVSLSALVTIAYFTASKWAIRHETLTFNDPVRGNRPV
                     AVDVAVRRDKEMQANAGMITLPVAILNHGNTVKFTEYSFLANIFAARGYLAISIQHDL
                     PTDPPMVTKVGELYVGRLPQYQRGIANIRFAVDEMKKVEPNADYDRLTMVGHSMGGDI
                     SMYFAKMYPDQIKKVVTLDNLRVPFMTEGKFKILSFRSKDPVFKADPGVVPNEEVCEK
                     SGITVVQTGYQHTDMSDRGPDTVKTTIQGMLDKFLEDDSPLKPVETNTPKITDEPGPV
                     ALYAPIKN"
     gene            2162..2929
                     /locus_tag="VN968_22145"
     CDS             2162..2929
                     /locus_tag="VN968_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXL31971.1"
                     /translation="MSGEPTKPKSAGEVSAGQKAESGTAGRLPEATPSASEDIAAFVA
                     KARAMSPHAAGAKGRLVFALDATMSRQPTWDMACALQADMFREAAALGSLDIRLVYYR
                     GLNECRATGWISDSAQLARLMGKIDCRGGNTQIGKVLSEARREAVASAVRAVVFVGDA
                     MEEAVDELCAKAGELGLLKVPVFMFQEGHDDVAEQAFREIARLTGGAWCRFDPGAAAQ
                     LRELLRAAAAYAAGGREALRLMSKTGSGAAKLLGQMK"
     gene            complement(3112..>4248)
                     /locus_tag="VN968_22150"
     CDS             complement(3112..>4248)
                     /locus_tag="VN968_22150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXL31972.1"
                     /translation="SQSWVLLAIPAFVFAGNLMERCGMSHALVELARALIGWVKGGLG
                     MSVIVVAYFFSDICGSKMAEVSALGSALMPPLTRAGYDRRDSASLIAAGTAMGMLVPP
                     AIFMIVIAQVTNTSAVALFVAGFVPAVVIMLCLMAVVYIRARQNDWPVDSRPSLKRLG
                     HAALHAAVPMVVPFVILAGFILGIITATEAGAVVAGYAFLAAKLYYRNVSWREMGKIA
                     YDSAILTAAVVFLLAVASVYQYLMGVSGVPQLLGQVLGPLKAHPWLFLIGTAAMSCLF
                     GMVLEGLPAAVVLIPVVFPIAETMGIDPIHFNIVQTAAVGIGLFLPPMGVGLLMALRF
                     ANLSVGQHFRTYVPYMLALFAGLLLIICIPEISLTLPRLAGLIK"
BASE COUNT          843 a         1325 c         1331 g          749 t
ORIGIN      
        1 tgtgttgggc aacgagaagg gcggttcggg taaatccacc accgccctgc acattgctgt
       61 ggccttgttg aaggcaggcc agcgtgtcgc caccatcgat ctcgattgtc gccagcaaag
      121 tttcacccgt tacatcaaca accgtcgcac ctgggcgcgt cgcaccggcc tcgatctgca
      181 acttccagtg cattgctgca tcaagcttgg cgagaccatg cagatcgccg acaacgaatc
      241 atctgagttc cagcaattca tggacgcggt gagtgcggtt gagcggactt tcgatttcat
      301 cgttatcgat accccggggt ccgacagcta tctgatgcgt ctggcgcact cgatggccga
      361 taccctggtg acgccgatca acgacagctt cctcgacttc gacgtcctgg ggaccgttga
      421 ccccgcgacc tatgccgtga ccggcgagag ccactatgcg gagatggtcc gcgataccag
      481 gcgcaagcgc cgccagctcg acggcgtcag caccgactgg atcgtggtcc gcaaccggct
      541 gtcgatgttg ggatcgcgca acaagcagct cgtcgccgat ggtctcaagg atttgtcgtt
      601 ccggcttggc ttccgctcga tcgatggttt tgccgaacgg gtggtttacc gcgagttttt
      661 tccgcgcggg ctgaccgcgc tcgacgacct cgacgagacc acactcggca cccgcccgag
      721 catgggccac gtgacggcgc gcgaggaggt cacgagtctc ttgcggcagt tgaaactgcc
      781 gctcgatgag cgcggccgcc gccgcgccgc gaaccgggcc gaatggttca tgcaggtcga
      841 caagccgctc gaggtccacg atatcctggg cgtctgaggt attgcggtaa gccggtactg
      901 cgcgccatgg aacgatctgc cgcgcgccac gttttcacca ttcgtttacc taagtgttga
      961 acaaaacctt gatttaagcc gccccaaagt agaccgggtg gcggaccttc atttgtgcac
     1021 ctgcacaatt agagggtgcg tcgcacaacg atcaggcggt gacttcacaa aatttactct
     1081 tcctgtcacg cctgcgtgac atataagatg catacgggac cgtagagtcc cgtgtaggga
     1141 cataggcctt aggggtcgcg gcgccgagga cgacaacatg aaacgtggaa ttgccatact
     1201 ggtttcgctt tccgcgctgg tgaccatcgc gtatttcacc gcgagcaaat gggcgatccg
     1261 ccacgagacg cttacgttca acgatccagt acgcggcaac cgtccggtcg ccgtcgatgt
     1321 cgccgtacgg cgcgacaagg aaatgcaggc gaacgccggc atgattacgc tgccggtagc
     1381 aatcctcaat catggcaaca ccgtcaaatt caccgagtac tctttcctgg cgaacatttt
     1441 tgccgcccgc ggctatctcg cgatcagcat ccagcatgat ctgccgaccg atccgccgat
     1501 ggtgacgaaa gtcggcgaac tctatgtcgg gcgcttgccg cagtaccaac gcggcattgc
     1561 caacatccgc tttgcggtcg atgaaatgaa aaaggtcgag ccgaacgcgg actatgatcg
     1621 tctgaccatg gtgggacact ccatgggtgg cgacatctcg atgtacttcg ccaagatgta
     1681 ccccgatcaa atcaagaagg tcgtgacgct cgacaatctg cgcgtgccgt ttatgacgga
     1741 gggcaaattc aagatcctgt cgttccgttc gaaggatccg gtgttcaagg cagatcccgg
     1801 tgtcgttccc aatgaggaag tctgcgagaa gtccggcatt acggttgtgc agaccggcta
     1861 ccagcacacc gacatgagcg accgcggtcc cgataccgtc aagaccacaa ttcagggcat
     1921 gctcgacaag ttcctcgaag acgacagccc gctcaagccg gtcgagacca acacgccgaa
     1981 aatcaccgac gagccggggc cggttgcgct ctacgcgccg atcaagaact gacggccggc
     2041 atcgcgcccc gatccaaagg tcgtatcaat gtccggcgtg accggaacaa gcgcagtcgt
     2101 cgcgcattga ccgctccctt atcccggccc acataagact cgctgcaatt cgcgagcgct
     2161 catgtcaggc gaaccgacca aacccaaatc cgcaggcgaa gtgtccgcag gccaaaaggc
     2221 tgagtccggc acagccggcc gattgccgga ggcgacgccg tcggcttcgg aagacattgc
     2281 tgcttttgtc gccaaggccc gcgcgatgtc gcctcatgcc gccggcgcca agggccggct
     2341 ggtttttgcg ctcgacgcca ccatgagccg gcagccgaca tgggatatgg cctgcgcgct
     2401 gcaggccgac atgtttcgcg aggcagcggc cctcggaagc ctcgatatcc gcctcgtcta
     2461 ctatcgcggc ctcaatgagt gccgcgccac ggggtggatt tccgacagcg cgcaactggc
     2521 aagattgatg ggcaagatcg attgccgggg aggcaatacc cagatcggca aggtgttgtc
     2581 ggaggcgcgg cgcgaggcgg tggcctccgc ggttcgcgcg gtggtgttcg tcggcgacgc
     2641 catggaggag gcagtcgacg aactctgcgc caaggcgggg gaactcggcc tgctcaaggt
     2701 gccggttttc atgtttcagg aggggcatga cgacgtcgcc gagcaggcgt tccgcgagat
     2761 cgcgcgtctg accggcggcg cgtggtgccg gttcgatccg ggcgccgcgg cgcaattgcg
     2821 cgagctgctg cgcgccgcgg cggcctatgc cgccggcggg cgcgaggcgt tgcggctgat
     2881 gtcgaagacc gggagcggcg cggccaagtt gctggggcag atgaagtgat ctcgatgcgc
     2941 caggcactac ccggttgcgg ttttcgctac ggcatcgagc gctttagtca tcatgccgac
     3001 cgcggcctcc gtctcgccgc tcagccgcca aggcgcgctt ttcgcttcgc ccgttttttt
     3061 ggcgggcgag gaccgtcatg ggtttcagtg cgaccgtctc cgcgtggcgc cttatttgat
     3121 cagccccgcc agcctcggca gcgtcaggct gatctccggg atgcagatga tgagcagcag
     3181 cccggcaaac agcgccagca tatacggcac ataggtacgg aagtgctgcc cgacgctcag
     3241 gttggcgaag cgcagcgcca ttaatagccc gacgcccatc ggtggtagga acaggccgat
     3301 gccgacggcc gcggtctgca cgatgttgaa gtggatcggg tcgatgccca tcgtttcggc
     3361 gattggaaac accacgggga tgagcacgac cgcggcgggc aggccctcca gcaccatgcc
     3421 gaacaggcaa ctcatggcag cggttccgat caggaacagc cacgggtgcg ctttcagtgg
     3481 cccgagcacc tggccaagca attgtggcac gccgctcacg cccatcaggt attgatagac
     3541 cgaggcgacc gcgagcagga acaccaccgc ggcggtgagg atcgcgctgt cgtaggcgat
     3601 cttgcccatc tcgcgccagg agacattgcg gtagtagagc ttggcggcga ggaaggcgta
     3661 acccgcgacc acggcgccgg cctcggtcgc ggtgatgatg ccgaggatga agccggcaag
     3721 gatcacaaac ggcaccacca tcggcacggc ggcgtgcagc gccgcgtggc cgaggcgctt
     3781 gaggctcggc ctggaatcga ccggccaatc gttctggcgc gcgcggatat aaaccactgc
     3841 catcaggcac agcatgatga caacagccgg aacgaagccg gcgacgaaca gcgccaccgc
     3901 cgacgtattg gtgacctgcg cgatcacgat catgaagatc gccggaggca caagcatgcc
     3961 catcgcggtg ccggcggcga tcagcgaggc tgaatcgcgc cggtcgtagc cggccctggt
     4021 cagcggcggc atcagcgccg aaccgagcgc cgagacttcc gccattttcg atccgcagat
     4081 gtcggagaag aaatacgcca ccacgatcac ggacatgccg agcccgccct tgacccagcc
     4141 gatcagggcg cgcgccagtt ccaccagcgc gtggctcatg ccgcagcgct ccatcagatt
     4201 gccggcgaac acgaaggcag ggatggcaag cagcacccag ctctgcga
//
LOCUS       DATNOI010000546         2586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_458104,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000546 DATNOI010000000
VERSION     DATNOI010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458104"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1881)
                     /locus_tag="VN968_22155"
     CDS             complement(<1..1881)
                     /locus_tag="VN968_22155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXL31973.1"
                     /translation="MSKPGLPRLEAQLRSNIAGDVLFDAFNRGRYATDASFYQIMPAG
                     VVVPRSVDEALRALAICRDEGVIVTPRGGGTSQCGQTVNDGIVVDLSKHLTRIVSLDA
                     ENRTCTVEPGIVLDDLNRQLRRHGLWFPVDVSTASRATIGGMAGNNSCGGRSLRYGTM
                     RDNTVSIDAALADGTLLHFGEVPRDLAQANSGDGGLELFRDMLDLGAREAAEIAERFP
                     KVQRRVGGYNLDALVPRNAPNNLAHLLVGSEGTLAFTTQVELKLWPVIRNKVLGVCHF
                     GSFYEAMDAAQHLVKLRPIAVELADRTMLSLGREIAMFQPIIATAVRGDPDAVLIVEF
                     AEEDQAENLARLKQLGELMADLGFGWDQPKRKWGGVVEISEPGLQTAIAEFRAAGLNV
                     MMSMKQEGKPVSFVEDCAVPLPHLADYTERLNAIFARHGTSGTMYAHASEGCLHVRPV
                     LNLKLEKDVKAMRAIAEEAFAMVREYKGSHSGEHGDGIVRSEFHGAMFGERIIGDFAE
                     VKHRFDPQGVLNPGKIVDPPKMDDRALFRYPPDYRVAELKTALDWSAYSGVGGGFQGA
                     VEMCNNNGACRKLEGGVMCPSYRATRNEKDVTRGRANTLRLAISGQLGPEALASDEMM
                     DTL"
     gene            complement(2122..>2586)
                     /locus_tag="VN968_22160"
     CDS             complement(2122..>2586)
                     /locus_tag="VN968_22160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31974.1"
                     /translation="IVGVKSAIRSAGDGVKKRGHRGEREVSRKTIARGMPGRSGVTVV
                     TNSHAFLFCVRGCGRIARPAFPAPSEFLGRKVLAKPRAHRAARSRSLVLKIGATSLRG
                     AQATITVIASQRVGAKRRPMTGYAKQSTARATVTMDCFVASLLAMTAAELAV"
BASE COUNT          462 a          835 c          804 g          485 t
ORIGIN      
        1 tagcgtatcc atcatttcgt cggaggccag cgcctccgga cccaactggc cggagatcgc
       61 gagccgcagc gtgttggcgc ggccccgcgt gacatccttc tcgttgcggg tggcgcgata
      121 ggacggacac atcacgccgc cctcgagctt gcggcaggcg ccattgttgt tgcacatctc
      181 gaccgcgccc tggaaaccgc cgccgacgcc ggaataggcc gaccagtcga gcgccgtctt
      241 cagctcggcg acgcggtagt ccggcggata gcgaaacagc gcgcggtcat ccattttggg
      301 cggatcgacg atcttgccgg gattgagcac gccttgcgga tcgaagcggt gcttgacctc
      361 ggcgaaatcg ccgatgatgc gctctccgaa catcgccccg tgaaattccg agcgcacgat
      421 gccgtcgccg tgctcgccgg aatgcgaccc cttgtactcg cgcaccatcg caaaggcttc
      481 ctcggcgatc gcgcgcatcg ccttgacgtc cttttcgagc ttcaggttca gcaccgggcg
      541 cacatgcagg cagccttcgg aggcgtgtgc atacatggtg ccgctggtgc cgtgcctggc
      601 gaagatggcg ttgagccgct cggtatagtc ggcgagatgc ggcagcggca ccgcgcagtc
      661 ctcgacgaag gagaccggtt tgccctcctg cttcatcgac atcatgacgt tgaggccggc
      721 ggcgcgaaac tcggcgatcg cggtctgcaa gcccggctct gatatctcga ccacgccgcc
      781 ccatttgcgc ttgggctggt cccagccaaa accgagatcg gccatcagct cgccaagctg
      841 cttcaggcgg gccaggtttt cggcctggtc ttcctcggca aactcgacga tcaggaccgc
      901 atcgggatcg ccgcgcacgg cggtggcgat gatcggctgg aacatcgcga tctcgcggcc
      961 taacgagagc atggtgcgat ccgccagctc gactgcgatt ggccgcagtt ttaccagatg
     1021 ctgggccgca tccatcgcct cgtaaaaact gccgaaatgg cagacgccca gcaccttgtt
     1081 gcggatcacc ggccacagtt ttaattcgac ctgggtggtg aaggcgaggg tgccttcgga
     1141 gccgaccagc agatgcgcca gattgttcgg cgcgttgcgc ggcaccagcg catcgagatt
     1201 gtagccgccg acccggcgct gcactttcgg gaatctttcg gcgatctcgg cggcttcgcg
     1261 cgcgccgaga tcgagcatgt cgcgaaacag ctcaagcccg ccatcgccgg aattcgcctg
     1321 cgccaaatcg cgcggcacct cgccgaaatg caggagcgtg ccgtcggcaa gcgctgcgtc
     1381 gatcgaaacc gtgttgtcgc gcatggtgcc gtaacgcagc gaacggccgc cgcaggaatt
     1441 gttgccggcc atgccgccga tggtggcgcg cgaggcggtg gaaacgtcga ccggaaacca
     1501 caggccatgc ctcctgagct gccggttgag gtcgtcgagc acgatgcccg gttctaccgt
     1561 gcaggtccgg ttctcggcgt cgagcgagac gatgcgggtg aggtgttttg agagatcgac
     1621 gacgatgccg tcattgacgg tctggccgca ttgcgaggtg ccgccgccgc gcggcgtcac
     1681 gatcacgccc tcgtcgcggc agatcgccag cgcccgcagc gcctcgtcta cgctgcgcgg
     1741 caccaccacg ccggccggca tgatctggta gaacgaggcg tcggtggcgt aacggccgcg
     1801 gttgaaggca tcgaacaaga cgtcgccggc gatattcgac cggagctgcg cttcaagccg
     1861 cggcaagcct ggtttcgaca tttttgaccc agtcggcggc tgcaaaacta actgctccgt
     1921 agttcgaaac cgcaggaaat gcaattattt tcgggcgttt cgggtttttg aattcaaaac
     1981 cggggtgagc gggaggctcc acaggctctg cccgcggatc gtcccctcgc ccgaagcctt
     2041 cggcttcgac ctttcctcgc aagcggggcg aggtgacccg aatcgcgcgt gccctgcgcg
     2101 cggcaattcc cgattcgatt ttcaaacagc caattcagcg gccgtcattg cgaggagcga
     2161 agcgacgaag caatccattg tcaccgttgc tctggctgtg gattgcttcg cgtagcctgt
     2221 catcgggcgg cgctttgcgc cgacccgttg gctcgcaatg acggttatcg tcgcttgcgc
     2281 tcctcgcaat gacgttgccc caattttcaa aacaagactc cgcgatctcg cggcgcgatg
     2341 cgcccgaggt tttgcaagaa ccttccgccc taaaaattca gagggcgcag ggaatgccgg
     2401 gcgcgcaatg cgcccgcagc ctcgcacgca aaataaaaaa gcatgcgagt tagtcaccac
     2461 ggtcacaccg gaacgacccg gcattccccg cgcaatggtt ttacggctta cttcgcgctc
     2521 tccccggtga ccacgctttt tgacaccgtc gcctgcgctt cttatcgcag acttgacgcc
     2581 aacgat
//
LOCUS       DATNOI010000547         4315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_763457,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000547 DATNOI010000000
VERSION     DATNOI010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763457"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            537..3707
                     /locus_tag="VN968_22165"
     CDS             537..3707
                     /locus_tag="VN968_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HXL31975.1"
                     /translation="MPNKMLIDATHPEETRVVVVRGNRVEEFDFETAQRKQLRGNIYL
                     AKVTRVEPSLQAAFIEYGGNRHGFLAFSEIHPDYYQIPVADRQALIEADERAHREAEE
                     ESENRSNRRRPRHRNSRRRGNGERIQSEIIENAGTDPSPAARPPEGHPHAEDLHTEDL
                     HPEDLPAEGLHTEHLHADDLHAHPDDAGHHGGDAEHHDHDDHEHDHAHEGHDHAHDEH
                     DHAPASGAEAAQFQPDTPVSAAAEASVATAPDAPAKVEAHGESAHDERTHQEHAEENA
                     AHAEDAASAEHAGEHQALPHDEHSAQPHGDENHEDDDDGDDAEEEVVESVGGDDVMEE
                     VPERAFRPRRQYKIQEVIKRRQVMLVQVVKEERGNKGAALTTYLSLAGRYAVLMPNTA
                     RGGGISRKITSAQDRSRLKEVVQDLDVPEGMGIILRTAGASRTKPEIKRDFEYLIRMW
                     ETVRDMTLKSQAPTLVYEEGSLIKRSLRDLYNKEIDEIQVAGEAGYQEARDFMKMLMP
                     SNVRAVRQYRDGQPLFSRMGVESQLDAMFSPTVQLRSGGYIVINQTEALVSIDVNSGR
                     STREHHIEDTALKTNLEASEEVARQLRLRDLAGLIVIDFIDMDEKRNNRSVERKLSDC
                     LRQDRARIQVGRISHFGLLEMSRQRIRASVLESSTEPCSQCGGSGHVRSVSSVALQLL
                     RGLEEILMKGATHNLVVRTRTDVALYVLNHKRGHLRDLENSFKVTLAVIADQTVSGQQ
                     SFIIDRGEQVHTLEAAKALLAAQVAAFPAYVEEASDDEEPFDIETESEVETEETEGLA
                     DDAPVSEASDGETDADGHKRKRRRRRRGRPGEARDGAPMRDESEIPSIASDAPAVAGA
                     IADDGESDEDEGEEQPGAARADQAAGGERRPRRRGRRGGRRRRGGPEDGLAGSIVDEL
                     GPIPVSEATDAVADFDGYSAEPSLPVVQPESIAPAPERQHADPVPQEAPRASMQEASP
                     ASVPEDTAQEAERAARRRSTVREKVSFMVDARPDAAAAAVNHSQPEPTTTSPAPAPAE
                     ETAPESTDDTQPRRAGWWSRRFGGGE"
     gene            3842..4171
                     /locus_tag="VN968_22170"
     CDS             3842..4171
                     /locus_tag="VN968_22170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31976.1"
                     /translation="MADRQSIRHIGLEMKAAADRGGLTFFGGCSMAKSATVGLSYGAL
                     RKECLDAVRQWPGCETIGGIQILRDAIPGGFSVRVTLYGTAEKKIADRAISCVQREKR
                     RHFHLND"
BASE COUNT          876 a         1400 c         1393 g          646 t
ORIGIN      
        1 cccggttgtg tcgcgttcag cctcccggtg cagcgccgga gccttcgaaa cttcggagat
       61 tccagcgtct ttccgatccc tccgcagcca gcgcagcgcg cggctgacaa ggagcggcgg
      121 tttcgagccc gagcggcgtc cggtcccagg tcaccagatt gctccaggga cggcttcaga
      181 cacggtataa ccgattatcc gggcctttag ggcccgatat gcgatggccg gcgagcgcct
      241 tggcgctgcg tcgggctttc agcgacagac aaggcggcgt ctttgccgcc cacgtgttca
      301 gacgggccga cgcttgatgc gccagacagt gttgccgacc gtgatccggg taagaactgc
      361 gccgaggcgg agcgaaagct ccacgcgacg ctttgctccg ggatcccgtt cggcccggcg
      421 ccccgggttg cgttttggcc ttcccctcac ccccgaatca ggtggaccgt cgcgtcaccc
      481 ggccgcgcga cacgcgctcc cggttgaacg cgccccccgc cgtcatgagt tccaaaatgc
      541 ccaacaaaat gttgatcgat gctacccacc cggaagagac ccgggttgtc gtggtccgcg
      601 gcaatcgcgt cgaagagttt gatttcgaga ccgcccagcg caaacaactg cgcggcaaca
      661 tctatctcgc caaggtcacg cgggtagagc cgtcgctgca ggcggccttc atcgagtacg
      721 gcggcaaccg ccacggcttt ctcgccttca gcgaaatcca tccggactat tatcaaatcc
      781 cggtcgccga tcgccaggcg ctgatcgaag ccgacgaacg cgcccatcgc gaggccgagg
      841 aggagagcga aaaccgctcc aaccgccgcc ggccgcgcca ccgcaactcg cgccgccgcg
      901 gcaatggcga gcgcatccag agcgagatca tcgagaacgc aggcaccgat ccgtcaccgg
      961 ccgcccggcc gcctgaaggt cacccacacg cagaagactt gcacacggaa gacctgcacc
     1021 cggaagactt gcccgccgaa ggcttgcaca ccgaacactt gcacgccgac gatttgcacg
     1081 cccaccccga cgatgccgga catcacggcg gcgacgcgga acatcatgat catgacgatc
     1141 atgagcatga ccatgctcac gaagggcacg accatgcgca cgatgagcat gaccatgcgc
     1201 cggcgagcgg ggcagaagcg gcgcagtttc agcccgatac gccggtttcg gcggcggcgg
     1261 aagcatctgt cgccactgcg ccggatgcgc cggccaaggt ggaagcccac ggcgagtccg
     1321 cccatgatga gcgcacccac caggagcatg cggaagaaaa cgccgcccat gccgaggacg
     1381 ccgcatcagc cgaacatgcc ggtgaacacc aggctttgcc tcacgacgag cattcggccc
     1441 aaccgcatgg cgacgagaac cacgaagatg acgacgacgg cgacgacgcc gaggaagaag
     1501 tcgtcgaatc cgtcggcggc gacgacgtca tggaagaggt gccggagcgc gccttccgtc
     1561 cgcgccggca atacaagatc caggaagtca tcaagcgccg tcaggtgatg ctggtgcagg
     1621 tggtcaagga agagcgcggc aacaagggtg cggcgctgac gacctacctc tcgctggccg
     1681 gccgctatgc ggttctgatg cccaacaccg cgcgcggtgg cggcatcagc cgcaagatca
     1741 ccagcgcgca ggatcgctcg cggctgaagg aagtggtgca ggatctcgac gtgcccgagg
     1801 gcatgggcat catcctgcgc accgcgggcg cgtcgcgcac caagcctgaa atcaagcgcg
     1861 acttcgaata tctgatccgc atgtgggaaa ccgtgcgcga catgacgttg aagtcacagg
     1921 cgccgaccct ggtctacgag gaaggctcgc tgatcaagcg ttcgctgcgc gatctctaca
     1981 acaaggagat cgatgaaatc caggtcgcgg gcgaagccgg ctatcaggaa gcccgcgatt
     2041 tcatgaagat gctgatgccc tcgaacgttc gcgcggtcag gcaatatcgc gacggccagc
     2101 cgctgttctc gcggatgggc gtcgagagcc agctcgacgc gatgttctcg ccgaccgtgc
     2161 aattgcgatc cggcggctac atcgtgatca accagaccga ggcgctggtt tcgatcgacg
     2221 tcaattccgg ccgttccacc cgcgaacatc acatcgagga taccgcgctc aagaccaacc
     2281 ttgaggcttc cgaagaagtc gcccgccagc tgcgcctgcg cgatctcgcc ggcctgatcg
     2341 tcatcgactt catcgacatg gacgagaagc gcaacaaccg ctcggtcgag cgcaagctca
     2401 gcgattgcct gaggcaggac cgtgcgcgaa tccaggtcgg gcgcatctcg catttcggcc
     2461 tactcgaaat gtcccgtcag cgcattcgcg ccagcgtgct ggagagttcg accgagccct
     2521 gctcgcagtg cggcggcagc ggtcatgtgc gttcggtatc gtcggtggcg ttgcagttgc
     2581 tgcgcggact cgaggaaatc ctgatgaagg gcgcgaccca caatctcgtg gtgcgcaccc
     2641 ggaccgacgt cgcgctttac gttctcaacc acaagcgcgg ccatctgcgc gatctcgaaa
     2701 acagcttcaa ggtgacgctg gccgtgatcg ccgaccagac cgtcagcggc cagcagtcgt
     2761 tcatcatcga tcgcggcgag caggtgcata cgctggaggc cgccaaggcg ctgctggcgg
     2821 cgcaggttgc ggctttcccg gcctatgtcg aagaagcctc cgacgacgaa gagccgttcg
     2881 acatcgaaac cgagtcggaa gtggaaaccg aagagaccga agggctggcc gacgatgcgc
     2941 cggtcagcga ggcgagcgac ggcgaaaccg acgcggacgg ccacaagcgc aagcggcggc
     3001 ggcggcggcg cggccgtccc ggcgaagcgc gcgacggcgc gcctatgcgt gacgagagtg
     3061 aaattcccag cattgcgtcc gatgcgccag cggttgccgg cgcgatcgcg gatgacggcg
     3121 aatccgacga ggacgaaggc gaggagcagc ccggcgcggc ccgcgccgat caggcggcag
     3181 gcggcgagcg aaggccccgc cgtcgcggac gccggggcgg tcgccgccgg cgcggcggac
     3241 cggaagatgg tctggccgga tcgatcgtgg acgaactagg gccgatacca gtttcggaag
     3301 cgacggatgc ggttgccgat ttcgacggct attcggccga accgtcgctg ccggttgttc
     3361 aaccggaatc cattgcacca gcgcccgagc gacagcatgc agatcctgtc ccgcaggagg
     3421 cgcctcgcgc gtccatgcag gaggcatctc ccgcatccgt accggaggac accgctcaag
     3481 aggccgaaag agccgcgcgg cgccgctcaa cagtacgcga aaaggtgagc ttcatggtcg
     3541 acgcccggcc ggatgcagcg gcggctgctg tcaatcacag ccagcccgag ccgaccacta
     3601 cgtcccccgc cccagcccct gcggaagaaa ccgcgcctga gtcgacagat gacacccagc
     3661 cgcgtcgcgc cggctggtgg tcgcggcgct tcggcggcgg cgaataagaa ttctggtttg
     3721 tgagacgaaa aaaccgcccg ggcaaaaccg ggcggttttg ttttgtcaaa agttgaatta
     3781 ggaactgtac tcgtaaagcc aaatcagcga gccgcgcctg gccatatccg ctctggagcg
     3841 catggcggac cgacagtcga ttcggcacat tgggcttgaa atgaaggcgg cggccgaccg
     3901 gggcggcctt actttctttg gcggctgcag catggcgaag tcggcgacgg tcggactaag
     3961 ctacggggcg ctccgaaagg agtgcctgga cgcagtcaga cagtggcccg gctgcgagac
     4021 catcggcggc attcaaattc ttcgagacgc aataccgggc ggtttttcgg tccgggtgac
     4081 gttgtacggc acggccgaga agaaaatcgc cgatcgcgcc atcagttgcg tccagcgcga
     4141 gaagcggcgg catttccatc tcaatgactg atcggagcag actctagacc atgatccgat
     4201 tggactgaat cggatcctaa tgccgcagca gggacagcac gagcacaagc accagcagcg
     4261 cggccacccc ctgccagaac agaacggggt tgcgttccag aagcgtccgc ggccg
//
LOCUS       DATNOI010000548         4905 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2262165,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000548 DATNOI010000000
VERSION     DATNOI010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2262165"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..956
                     /locus_tag="VN968_22175"
     CDS             324..956
                     /locus_tag="VN968_22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL31977.1"
                     /translation="MAKAQSPKSPSNRAERTAERREAIIEAALDEFISRGFTATRIDD
                     VARRAGVAKGTIYLHFKDKESMFEELIRTALVPLIGRLHAPPPIGGSVRDAVEGFART
                     FIQEVASTRRGDIVRLIVAEGPRFPSIADFYYREVVSRGLAGMRALIELAVSRGEIQQ
                     KNLARFPQILVAPAIIAVIWQSLFSRHAPLDATEMFRVHLDLIFGERRTT"
     gene            953..1741
                     /locus_tag="VN968_22180"
     CDS             953..1741
                     /locus_tag="VN968_22180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXL31978.1"
                     /translation="MTSSRMSSRILAALALAAVLAGCNQRKDPGYQGWVEADMIFVSP
                     DESGRVIKLDVREGDEVKQGAPLYSVDDDLQKADLNQNNATLANAQQTYDRAASLNKT
                     GSGTQANLDSAVSALRVAEARVNTSQTRLTRRGGFAPVSGTIQQIYFREGEMVPAQRP
                     VLSIMPPGNMKVRFYVPEPDLPKLAVGDEVRVTCDNCAGDLTAKIYFIATSAEYTPPV
                     IYSLDERNKLVYLIQARPSRPDTLRVGQPISVFLGAKIPVADKR"
     gene            1738..2682
                     /locus_tag="VN968_22185"
     CDS             1738..2682
                     /locus_tag="VN968_22185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL31979.1"
                     /translation="MTAAPVSTSDIAIDVHGLSKSFNGREVVHELSMQVKRGTIYGFL
                     GPNGSGKTTTIRMLCGLLTPDRGEGTCLGYDIRRDADKIKRQVGYMTQRFSLYQDLSV
                     RENLEFVARLYGVRDARGIASDMIKRLGLTGREEQLAGELSGGWKQRLALGACTLPNP
                     QLLLLDEPTAGVDPKARRDFWNEIHALAAEGLTVLVSTHYMDEAERCHEIAYIAYGHL
                     LVHGTVDEVIAKSALSTYTVTGDDLHKLADELTGKPGIDMVAPFGTSLHVSGRDKAAL
                     EATIAPYREAHGWHWQHGEPSLEDVFIELMTRSKDNFQ"
     gene            2679..3845
                     /locus_tag="VN968_22190"
     CDS             2679..3845
                     /locus_tag="VN968_22190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL31980.1"
                     /translation="MSVVSTPDHSRDTREPPFGFWRRSFAMVVKEFIQLRRDRVSFAM
                     IVMIPVMQLLLFGFAINTTPRNLPTAVLLQEDSDLARSILKALENTAYFHFTREVHDV
                     AEFDNLLLSGKVLFGVEIPRGFERAVRRGDHPALLVAADATDPVAAGSALGALGVVVQ
                     TALEHDLHTGDPPSLPFEIRTHARYNPAGSSRLNIVPGLVGTILTMTMLIFTALSVTR
                     EIERGTMESLLSMPITPVEVMLGKIVPYVLVGFIQATLIIGIGVLLFGVPVNGSLPLL
                     ALLSTLFITTNLSIGYTFSTIAQNQLQAMQMSLMFFLPSILLSGFMFPFAGMPVWAQY
                     IGEGLPLTHYVRIVRAIMLKGATLPNLQYDTIALVALMLMAMTIAVMRFRRTLD"
     gene            4011..>4905
                     /locus_tag="VN968_22195"
     CDS             4011..>4905
                     /locus_tag="VN968_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL31981.1"
                     /translation="MAGFWRKKKQGQDGAVSADFRHALMQQVMRTELIRIKALIGTTS
                     LLAIILWTVYLLAPEAVSRIWHGNLRPVYLYAIIVPFILFELSVHAVISRHVRLDRDI
                     PVIRRYIGALIETSMPTIALALHINSMGEVAALGFVTPMTYFIFIILSTLRLDFWLST
                     FTGVVAAVELFCMAAFYHPSGVIDAEPSVYYHAARSLIILICGALAGAVGVQLRRQFE
                     ASILAATARDRITNLFGQHVSPQVVERLIAEGASTNSDIRHVAVMFVDFRSFTAGARS
                     RSPQEVVDRLDGAFAVLVDILD"
BASE COUNT          884 a         1547 c         1531 g          943 t
ORIGIN      
        1 ggggaggagt cgtgagactc ccccctatcc cgatcgcccc ggaatgacgg ttctcaactg
       61 aattgcttcg tccgctttgc ttctcgcaat gaagaatggg ggcaacagat tctccgataa
      121 cggcgatgtg acaatcggcg cggtcggcct gcattgacaa tgactgacca gtcagtcatg
      181 taaaggccat gtcaaacacg cgaacaggtt ccggaacctc aaggggagcc ccgtcgaagt
      241 cggccattgg ccgacgcggg gataaggctg ccattggcgg acaaggggag aaggccgcct
      301 cggtcaagac gccaaagccc gccttggcaa aggctcagtc gccaaaatct ccatcgaacc
      361 gcgcggaacg cacggcggag cggcgtgaag ccatcatcga ggccgcgctg gatgaattca
      421 tctcgcgcgg atttaccgcg acgcggatcg acgacgtcgc caggcgggcc ggcgtcgcca
      481 agggcacgat ctacctgcac ttcaaggaca aggagtcgat gttcgaggaa ctgatacgga
      541 cggcgctggt gccgctgatc ggccggctgc acgcgccgcc acccatcggc ggatcggtgc
      601 gcgatgcggt cgagggtttc gcccggactt tcatccagga ggtcgcgtcc acccggcgcg
      661 gcgacatcgt gcgcctgatc gttgcggagg gcccgagatt tcccagcatc gccgatttct
      721 actatcgcga agtggtttcg cgcggcctcg ccggcatgcg cgccttgatc gagctcgcgg
      781 tttcgcgcgg cgaaatccag cagaagaatt tggcccgctt tccgcagatc ctggtggcgc
      841 ccgcgatcat cgcggtgatc tggcaaagcc tgttcagcag acatgcgccg ctggatgcga
      901 ccgagatgtt tcgcgtccat ctcgatctga tttttggcga acggaggacg acatgacctc
      961 gtcgcgaatg agttcgcgaa ttcttgcggc gcttgcgctg gcggcggtgc tggccggctg
     1021 caaccagcgc aaggatcccg gctaccaggg ctgggtcgaa gccgacatga tctttgtcag
     1081 ccccgacgaa tccgggcgcg tgatcaaact cgatgtgcgc gagggtgacg aggtcaagca
     1141 aggcgcgccg ctttattcgg tcgatgacga tctgcagaag gccgatctca accagaacaa
     1201 cgccacgctc gccaacgccc agcaaaccta tgaccgcgcg gcgtcgctga acaagaccgg
     1261 ctccgggacg caggccaatc tcgactccgc ggtgtcggcg ctgcgcgtgg ccgaggcgcg
     1321 ggtcaacacc tcgcagacgc gactgacccg ccgcggcggc tttgcgccgg tcagcggcac
     1381 cattcagcag atctatttcc gcgagggcga aatggtgccg gcgcagcggc cggtgctgtc
     1441 gatcatgccg cccggcaata tgaaggtgcg gttttacgtg ccggagccgg atctgccgaa
     1501 gctcgcggtc ggcgacgagg tgcgcgtgac ttgcgacaac tgcgctggcg atctcaccgc
     1561 caagatctat ttcattgcca catcggccga gtacaccccg ccggtgatct acagcctcga
     1621 tgagcgcaac aagctcgtgt acctgatcca ggcgcggccc tccagacccg acaccttgcg
     1681 tgtcggccag ccgatcagcg tgtttctggg cgccaagata ccggtagcgg acaagcgatg
     1741 accgcggcgc cagtgtccac ctccgacatc gcgatcgacg ttcacggcct gtcgaagtcg
     1801 ttcaatggcc gagaggtcgt gcatgagctg tcgatgcagg tgaagcgcgg caccatctac
     1861 ggcttcctcg gccccaacgg ctccggcaag accacgacca tccgcatgct gtgcggcctg
     1921 ctgacgcccg accgcggcga gggcacctgt ctgggctacg acattcgccg cgacgccgac
     1981 aagatcaagc gccaggtcgg ctacatgacg cagcgttttt ccctgtacca ggatctgtcg
     2041 gtgcgggaga atctggaatt cgtcgcaagg ctctatggcg tgcgcgatgc acgaggtatc
     2101 gcgagcgaca tgatcaagcg gctcgggctg acggggcgcg aagaacagct cgccggcgaa
     2161 ctctccggcg gctggaaaca gcgccttgcg cttggcgcct gcacactgcc caatccgcaa
     2221 ttgctgctgc tggacgagcc gaccgccggc gtcgacccaa aagcgcggcg cgatttctgg
     2281 aacgagatcc acgcgctggc ggcggaaggg ctgaccgtgc tggtctccac ccattacatg
     2341 gacgaggccg agcgctgcca cgagatcgcg tatatcgcct acggacatct gctggttcac
     2401 ggaaccgtgg acgaggtgat cgcgaaatcg gcgctgtcga cctacacggt caccggcgac
     2461 gacctccata agctcgcgga cgaactcacc ggcaagcccg gcatcgacat ggtggcgccg
     2521 ttcggaacca gcctgcacgt gtccggccgc gacaaggccg cgctcgaggc caccatcgcg
     2581 ccctatcgcg aggctcacgg ctggcattgg cagcacggcg agccgtcgct ggaggatgtg
     2641 ttcatcgagt tgatgacccg ctcgaaggat aatttccaat gagcgtcgtc agcacacccg
     2701 accacagccg cgacaccagg gagccgccgt tcggcttctg gcggcgcagt tttgcgatgg
     2761 tggtcaagga attcatccag ctcaggcgcg accgggtgtc gttcgcgatg atcgtgatga
     2821 tcccggtcat gcagcttctg ctgttcggct ttgccatcaa caccacgccg cgcaacctcc
     2881 ccacagcggt gctgctgcag gaggacagcg accttgcgcg ctcgatcctc aaggcgctgg
     2941 aaaacaccgc ctattttcac ttcacccggg aagtgcacga tgttgcggag ttcgacaatc
     3001 tcctgctgtc gggcaaggtg ctgttcggcg tcgaaatccc gcgtggcttc gagcgggcgg
     3061 tgcggcgcgg cgatcatccg gcgctgctgg tcgcggccga tgcgaccgat ccggtggcgg
     3121 caggctccgc gctgggcgcg ctcggtgtgg tggtgcagac cgcattggag cacgatctgc
     3181 acaccggcga tccgccgtcg ctgccattcg agatccgcac ccacgcccgt tacaaccccg
     3241 cagggtcgtc gcggctcaat atcgtgccgg gcctggtcgg caccatcctg accatgacca
     3301 tgctgatctt caccgcactc tcggtgacgc gcgagatcga acgcggcacc atggaaagcc
     3361 tgctgtcgat gccgatcact ccggtcgagg tgatgcttgg caagatcgtt ccttacgtgc
     3421 tggtgggttt catccaggcc acgctgatca tcggcatcgg cgtgctgctg ttcggggtgc
     3481 cggtgaacgg tagtctgccc ctgctggcgc tgctctcaac cctgttcatc acgacaaatc
     3541 tttcgattgg ctataccttc tcgaccatcg cgcagaacca gctgcaggcg atgcagatgt
     3601 cgctcatgtt tttcctgccg agcattctgc tgtcgggctt catgtttcca ttcgccggca
     3661 tgccggtatg ggcgcaatat atcggcgaag gcctgccgct gacgcattac gtgcggatcg
     3721 tccgcgccat catgctgaag ggggcgacgc tgccgaatct gcaatacgat acgattgccc
     3781 tggtggcatt gatgctgatg gccatgacca tcgcggtgat gcgcttccgc cgcacgctcg
     3841 attgatgcag aatgctcacc gtcattccgg ggcgggagcc ttgcggcaaa attgatgcga
     3901 gcatcaattt tgcgctgggc gagcgaatcc ggaatctcga gattccccga tgtgcaattg
     3961 cacatctgag gtccacgctt gcgcatgtcc cggaatgaca aggcaggata atggctgggt
     4021 tctggcgcaa aaagaaacaa gggcaagacg gcgcggtgtc agccgacttc cggcatgcgc
     4081 tcatgcaaca ggtgatgcgt accgagctga tccggatcaa ggcgctgatc gggacgacgt
     4141 cgctgctcgc catcatcctc tggaccgtct accttctcgc gcccgaggcg gtgagccgca
     4201 tctggcacgg caatctcagg ccggtctatc tttatgcgat catcgtcccg ttcatcctgt
     4261 tcgaattgtc ggtccatgcg gtcatcagcc ggcatgtcag gcttgatcgc gacatccccg
     4321 tgatcaggcg gtatatcggc gcgctgatcg aaacgtcgat gccgaccatc gcgctggcgc
     4381 tgcatatcaa tagcatgggg gaggtcgcgg cgctcggctt cgtaacgccg atgacttatt
     4441 tcattttcat cattctctcg acgctgcggc tcgatttctg gctatcgact ttcaccggcg
     4501 tcgttgccgc ggtggagttg ttttgcatgg cggctttcta tcatccttcc ggcgtcatcg
     4561 atgccgagcc cagcgtatac tatcacgccg cccgcagcct gatcatactg atttgcggcg
     4621 cgctggcggg cgcggtcggc gttcagttgc gccgccagtt cgaggccagc attctggctg
     4681 cgaccgcgcg cgaccgcatc accaatctgt tcggccagca cgtctcgccg caggtggtcg
     4741 agcgattgat cgccgagggc gccagcacca acagcgacat ccgccatgtc gctgtcatgt
     4801 tcgtcgattt ccgcagcttt accgctggcg cccgctcgcg ttcgccgcag gaggtggtgg
     4861 atcgtctcga cggcgcgttc gccgtgctgg tcgatatcct cgacc
//
LOCUS       DATNOI010000549         3591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2019676,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000549 DATNOI010000000
VERSION     DATNOI010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2019676"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VN968_22200"
     CDS             complement(<1..578)
                     /locus_tag="VN968_22200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HXL31982.1"
                     /translation="MIYDIRHVTTYNYESPIGFARCSLRLEPRSGDGQRLVSHSVDIR
                     PRPAERTIRHDFFGTHTESVLIEVPHRTLRIDSRSRVSVYRHAPDRAMRGPTWEDVRD
                     LAFEANALGPTSPIGYVFASPLVPVQQPVTGYAALSFPPASGILAGAVGLMHRIRSDF
                     KYDPKATVISTPLNEVFEKRHGVCQDFAHVMIA"
     gene            complement(575..3019)
                     /locus_tag="VN968_22205"
     CDS             complement(575..3019)
                     /locus_tag="VN968_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HXL31983.1"
                     /translation="MRDYVRLPGIPDEFIAPDGAPRAVWSRFFDAFATLAPAEIERRF
                     GSADRHLREAGVTYRAPGETADRLWPLSHLPLLIDETDWQQLTAGIVQRAELLELVLG
                     DLYGEGRLIAEGAIPAAAIAGSTEYLRPVCGIKPPGGRYLALYAADVGRGPDGRWWVL
                     GDRTQAPSGAGYALENRLVLSRAFTALYKSMNVERVAPFFEAFRDSLRASADRDEPRI
                     GLLTPGQFSETYFEHATLARYLGFLLVEGDDLAVSGDRIHIRTVAGLKRLDVLLRRVD
                     SNSLDPLELDASSQLGVPGLIDVLRKNGVVVANMPGSGVMEARALLGFLPSLSRRFFG
                     EDLKMPHIATWWCGQKTAREEVLSRLDEFAIEGAYRRGVPGFPGNGPVLAGELSRSER
                     ERLKAAINDRGIDYVGQELVRLSTTPVWDQGRITPRPFVLRVFAAATPDGWTIMPGGF
                     CRIADQLDARAVSMGDGARAADVWVVSDKTVSTASLLPPMDTVRIRRIAGVVPSRAAD
                     NLFWLGRYLERAEATLRLVRALGTSQRDAGKGPSTVPHSVERIHRLLVTWGASSQASR
                     TQSAKIVAEALQAGDKFGSALSLLRAAQRTATSLRERLSPDAWQVITEMTERLAEEVE
                     DDDGVVSAAELTLQELASFAGLAQENMNRAAGWRFLEMGRRAERAVNTVRFARQFAYD
                     EAGGEDLDILLTLVDCQITYRSRYLVGPLLAPVRDLVVLDPYNPRSVAFQVFALNDHI
                     ASLPTLKEGGLIERPQRLAVALQATLTTSEAASLDTKTLFALEQDLLNLAEAIGLHYF
                     PHGPNASRPEKLTGLA"
     gene            complement(3147..>3591)
                     /locus_tag="VN968_22210"
     CDS             complement(3147..>3591)
                     /locus_tag="VN968_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HXL31984.1"
                     /translation="VRYVDSSVERLQVKAGGFVEGRHVVTCNGRRMPMTETGRSGEAV
                     AAVRFKAWQPASGLHPTIPVHAPLTFDLIDTWNGRSLGGCVYHVAHPGGRSYDSKPVN
                     SYEAEARRLARFQDHGHTPGKIDPPREERTIEFPLTLDLRRPLLH"
BASE COUNT          661 a         1212 c         1132 g          586 t
ORIGIN      
        1 gcgatcatca catgcgcaaa atcctggcaa acgccgtggc gcttttcgaa cacttcgttg
       61 agtggcgtcg agatcacggt cgctttcggg tcgtacttga aatcgctgcg gatccgatgc
      121 atcaggccga ccgcgccggc aagaattccg cttgccggcg gaaagctcaa cgccgcatat
      181 ccggtaaccg gctgctgcac cggcaccagt gggcttgcaa acacgtagcc gatcggcgac
      241 gtggggccga gagcgttggc ctcgaaggcc agatcacgaa cgtcttccca cgtgggaccc
      301 cgcatcgcgc ggtcaggagc gtggcggtaa accgagaccc gcgatcgtga atcgatccga
      361 agggtacgat gggggacctc gatcaggacg ctctcggtat gcgtaccgaa aaagtcgtgc
      421 cgtatcgtgc gctccgcagg ccgcggccgg atatcgacgc tgtgcgagac caatcgttgt
      481 ccgtcgccgc tcctcggctc cagccgcagc gagcatcgcg caaaaccgat cggactttcg
      541 taattgtagg tggtgacgtg acgaatgtcg tagatcacgc gaggcccgtc agcttttcgg
      601 gccggctcgc attgggtccg tgcggaaagt aatgcaatcc gatggcctca gcgagattga
      661 gcaggtcctg ctccagcgcg aacagcgtct tggtgtcgag gcttgccgcc tccgacgtcg
      721 tcagcgtcgc ctgcagggcc acggcgagac gctgcggacg ctcgatcagt ccgccctcct
      781 tcaaggtcgg caggctagcg atgtgatcgt tcaacgcaaa cacctgaaag gcgaccgacc
      841 ttggattgta gggatcgagc accacgagat cgcgaactgg cgccagtagc ggcccgacca
      901 gatagcgcga gcggtaggtg atctggcaat ccaccagcgt cagcaggatg tcgaggtcct
      961 cgccgccggc ctcatcatag gcaaactgac gcgcgaaacg cacggtgttg acggcgcgtt
     1021 cggcgcggcg gcccatttcg agaaaacgcc agccggcggc gcggttcatg ttttcctggg
     1081 ccaggcctgc gaagctcgcg agctcctgca gcgtcagttc ggcggcgctg acgacgccgt
     1141 cgtcatcttc aacctcttcg gcaagccgct ccgtcatctc ggtgatgact tgccaggcat
     1201 ccggcgaaag ccgttcacgc agcgacgtcg cggtgcgctg cgccgcccgc agcagcgaca
     1261 acgccgagcc gaacttgtcc ccggcctgca acgcttcggc gacaatcttc gcagactgtg
     1321 tccgcgacgc ctgcgagctc gcgccccatg tcaccagaag cctatgaatt cgttccaccg
     1381 aatgaggcac ggtggacggc cccttgccgg catcgcgctg cgacgttcca agcgcccgca
     1441 ccaaccgcaa cgtcgcttcg gcgcgctcga gatagcgtcc gagccagaac agattgtccg
     1501 ctgcccggct cggaaccacg ccggcgatgc gtctgatccg taccgtatcc atcggcggca
     1561 gcaggctggc cgtcgaaacc gtcttgtcgg agaccaccca gacatcggcc gcccgcgcgc
     1621 catcccccat cgacaccgcg cgggcatcga gctggtcggc gatccggcag aagccgccgg
     1681 gcatgatggt ccagccgtcg ggagttgcgg ccgcgaaaac ccgcagcacg aacgggcgcg
     1741 gcgtgatccg tccctggtcc cacaccggtg tcgtcgacag ccgcaccagc tcctggccga
     1801 cataatcgat gccgcgatcg ttgatcgctg ccttgagccg ttcgcgctcg cttctcgaca
     1861 actcgccggc gagcaccggc ccattacccg gaaagccggg gacgcctctg cgataagcgc
     1921 cctcgatcgc aaattcgtcg agccgcgaca atacttcctc gcgcgcggtc ttctggccgc
     1981 accaccatgt cgcgatgtgc ggcatcttca gatcctcgcc gaaaaaccgc cggctcaggc
     2041 tgggcagaaa gcccaacagc gcccgcgcct ccatcacgcc tgagcccggc atattggcca
     2101 cgacgacgcc gttcttgcga agcacatcga tcaggccggg cacgccgagc tgcgaggaag
     2161 catccagctc cagcggatcg agcgaattgg aatcgacccg gcgcagcaac acgtcgagcc
     2221 gcttcaggcc ggcgacggtg cggatatgga tgcggtcgcc actgacggcg agatcgtcgc
     2281 cctcgaccag gagaaatccg agatagcgcg ccagcgtggc gtgctcgaaa taagtttcgc
     2341 tgaactggcc gggcgtcagc aatccgatgc gcggctcgtc gcggtcggcg ctggcgcgca
     2401 gactgtcgcg gaacgcctcg aagaacggcg cgacccgctc cacattcatc gatttgtaga
     2461 gcgcagtgaa agcgcgcgac agcaccagcc ggttttccag cgcgtagccg gcgccggacg
     2521 gagcttgggt gcggtcgccc agcacccacc agcgcccgtc gggcccgcgg ccaacatcgg
     2581 cggcatacag cgcaagatag cggccaccgg gtggtttgat gccgcaaacc gggcgcagat
     2641 attcggtgct gccggcaatc gctgccgcgg gtatcgcacc ctcggcaatc aatcggccct
     2701 cgccatagag gtcgccgaga accaactcca gcaattccgc gcgttgcacg atgccggcgg
     2761 tcaattgctg ccagtcggtc tcgtcgatca ggagcggcag atggctcagc ggccaaagcc
     2821 ggtcagcggt ttcgccgggg gcgcggtagg ttacgccggc ttcccgcaaa tgccggtcgg
     2881 cggatccgaa acgccgctcg atttccgcag gcgccagcgt cgcgaaggca tcgaagaaac
     2941 ggctccaaac cgcacgcggc gcgccgtcgg gcgcgatgaa ctcatcgggg attcccggca
     3001 gccgaacata gtcgcgcatc cactgcgcca cgcgccgatt ggctgggcgg gccttggcgc
     3061 cctgactgcc gacccgatcg cttgctcgag tgctttcttg gccgtcgtgc cccgccatcc
     3121 cgactctcct gcccaaagcc catccctcaa tggagcagcg gcctcctcaa gtcgagggtc
     3181 aaaggaaatt caattgtgcg ttcctcgcgc ggcggatcga tcttgccggg ggtatggcca
     3241 tgatcctgaa accgggcaag ccgccgcgcc tcggcctcat aggaattgac cggtttgcta
     3301 tcataactgc gtccgccggg gtgggcgacg tgatagacgc agccgcccag cgagcggccg
     3361 ttccaggtat cgataagatc aaacgtcagc ggcgcatgaa ccggtatcgt cgggtgcagc
     3421 ccggaggccg gctgccaggc cttgaagcgg acggccgcga ccgcttctcc tgaacggccg
     3481 gtttccgtca tcggcatccg ccggccgttg caggtgacga cgtggcggcc ttcgacgaag
     3541 ccgccggcct tgacctgcaa ccgctcgacc gacgaatcga catagcgcac g
//
LOCUS       DATNOI010000550         4751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_763226,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000550 DATNOI010000000
VERSION     DATNOI010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763226"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /gene="hydA"
                     /locus_tag="VN968_22215"
     CDS             complement(<1..1180)
                     /gene="hydA"
                     /locus_tag="VN968_22215"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HXL31985.1"
                     /translation="MTEPAYDIVIRNGRVATASDVFEADVAITGETVAAIGRGLAAGR
                     REIDARGKLVLPGGVDSHAHIEQLSAAGIMNADTFETATTSAAFGGTTTVIPFAAQHV
                     GMALPQVVADYHALAKRGAVIDYAFHMIIADPTPETLQNDIPELVKQGHGSIKIFMTY
                     DLLKIDDEPLLDVLIAARESGAMLCAHAENHGIIAWMVKRLLARGYTHPKYHAISHAR
                     FSEADAFNRLIGMAALVDQPIMIFHVSTAEGAKVIRDARGQGLKVFAETCPQYLFLTA
                     DDLDKPGVDGAKWMCSPPPRRIADQDALWQALALGDLQTVSSDHAPYRFDETGKLRAG
                     ANPNFKQIANGMPGLQVRLPLLFDAMVSKGRLGLEKFVELTATAPAKIYNLHPRKGSI
                     A"
     gene            1309..1971
                     /locus_tag="VN968_22220"
     CDS             1309..1971
                     /locus_tag="VN968_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HXL31986.1"
                     /translation="MPRPRILVINPNSNVVVTRGLEAALQPLDFADGPEIVCTTLDGG
                     PYGVESQADVDSVTMPLRRLVESDNGSAAFVIACYSDPGLHVCREGTDRPVFGIAECG
                     VLTAMTRAETFGVIAIAQCSIRRHIRYLRQMGLMDRLAGERPLNMSVAETASGDGTLA
                     KMIEVGRALKDEDGAGAIVMGCAGMARLRRPLEDALGIPVIDPTQAAVAMALGAVQFS
                     RH"
     gene            complement(2006..2938)
                     /locus_tag="VN968_22225"
     CDS             complement(2006..2938)
                     /locus_tag="VN968_22225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HXL31987.1"
                     /translation="MRVINVAAAQMGPIQRADSREVVVKRMLALMDEARAGGADLIVY
                     PELALTTFFPRWYMEDQAEVDTWFEREMPNTASMPLFARAAEYQMAMSFGYAELTPDG
                     HHFNTCILTDKSAKIVGKYRKVHLPGHSEFDTERAFQHLEKRYFEPGDIGFDVWRAMG
                     GIIGMAICNDRRWPETYRVMGLQGVEMVLLGYNTPAVNSQKSEEGPEKRLFHNRLSVQ
                     SGAYQNATWVVAVAKAGVEDGFPLIGGSLIVDPNGEIVAEASTEGDEVLVHPCDLDAT
                     SFGKKTIFDFARHRRIEHYGLITSRAGAVPPPET"
     gene            3450..4700
                     /locus_tag="VN968_22230"
     CDS             3450..4700
                     /locus_tag="VN968_22230"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HXL31988.1"
                     /translation="MTGSNRVDHIRITSHPAPGAKHDYPIDWGAASARERGPVIGTVS
                     RPQDRNVIGTHGGSYSVYRALAVSSGALDPIRRPDLTNTHPAETIGPFSQWADPQRIV
                     SLDPWGHLIAENFAKEIAEGIDIRPSIAVTRARLDLPELQAAIEAGRLKHDGEVVHKN
                     GSVSVVKIAIDPVWYLPGMAARFATTENNLRRQLFEQTAGMFPELVTRPDLQVFLPPI
                     GGTTAYLFGDVTKLPDHRTRITCRVHDECNGSDVFGSDICTCRPYLLHGIEECARAGQ
                     TGGLGIIIYNRKEGRALGEVTKFLVYNARKRQEGGDAAAQYFERTECVAGVQDARFQQ
                     LMPDVVHWLGLKRIDRFVSMSDMKHDALTGQGIEIVERVPIPDDLVPADAHVEIAAKK
                     AAGYYTSEPEQNPVNPVGRSLEKY"
BASE COUNT          919 a         1452 c         1465 g          915 t
ORIGIN      
        1 cagcgataga gcccttgcgc ggatgcaaat tgtagatctt ggcaggtgcg gtcgccgtca
       61 actcgacaaa tttttccagg cccaggcggc ccttcgacac catcgcgtcg aacagcagcg
      121 gcaggcgcac ctgcagtccc ggcatgccgt tcgcgatctg cttgaagttg ggattggcgc
      181 cagcgcgcaa tttaccggtc tcgtcgaaac gatacggcgc atggtcggac gaaacggtct
      241 gcagatcgcc gagcgcaagc gcctgccaca gcgcgtcctg atcggcgatg cggcgcggcg
      301 gcgggctgca catccatttg gcgccgtcta cgcccggctt gtccagatcg tcggcggtga
      361 gaaatagata ttgcgggcag gtctcggcga agactttcag gccctgcccg cgcgcatcgc
      421 ggatcacctt ggcgccctcg gccgtcgaca cgtgaaagat cataatcggt tgatcgacga
      481 gagcagccat gccgatcagc cggttgaagg cgtcagcttc cgaaaacctc gcatggctga
      541 tggcgtggta tttcgggtgg gtgtaaccgc gcgccagcag ccgcttcacc atccatgcga
      601 tgatgccatg gttctcggca tgggcacaca gcatggcgcc ggattcacgt gccgcgatca
      661 gtacgtcgag caaaggctcg tcatcgatct tcagcaagtc atatgtcatg aagatcttga
      721 tcgagccgtg gccctgtttg accaactccg gaatgtcgtt ctgcagggtc tctggagtcg
      781 gatcggcgat gatcatgtga aacgcgtagt cgatcaccgc gccccttttc gcgagcgcgt
      841 gataatccgc caccacctga ggcagcgcca tgccgacatg ctgggcggcg aacgggatca
      901 cggtggtggt gccgccgaaa gctgccgatg tcgtggcagt ctcgaacgta tcggcattca
      961 tgatgcccgc agccgagagc tgttcgatat gagcgtggct atcgacaccg ccgggcagaa
     1021 ccagcttgcc gcgcgcgtcg atctcgcgcc tgccggccgc caggccacgg ccgatcgcgg
     1081 cgacggtctc gcctgttatg gcgacgtcgg cctcgaatac atcgctggcg gttgcgacac
     1141 gtccgttgcg gatcacgatg tcataggcag gttctgtcat gaacggctcc ggatcgctaa
     1201 gccaccttcc cgcacttgtg aaggaatggc tgacaacggg cgaaacgaga aatcgctgga
     1261 cggcgccaga ctgacgttcg aaactatgcc acttgcaagg aagaaaccat gccccgcccg
     1321 cgcatcctcg tcatcaatcc aaattcgaac gtcgtcgtca ccaggggact ggaggcggcc
     1381 ttgcagccgc tcgatttcgc ggacgggccg gagatcgtct gtacgacgct ggacggggga
     1441 ccttacggtg tggagagcca ggccgatgtc gacagtgtta cgatgccgct gcgccggctg
     1501 gtcgagagtg acaacggctc ggcagccttc gtgatcgcct gctacagcga cccaggtctg
     1561 catgtctgcc gcgaaggcac ggatcgaccg gtattcggca tcgcggagtg cggcgtgttg
     1621 acggcgatga cgcgcgcgga aacttttggc gtgatcgcca tcgcgcaatg ctcgatccgc
     1681 cggcacatcc gttatctccg ccagatggga ctgatggacc ggctggcggg cgagcgcccc
     1741 ctcaacatga gcgtggccga aacggcgtcc ggcgacggaa cgctggcgaa aatgatcgag
     1801 gtcgggcgcg cgctgaagga tgaggacggg gccggcgcga tcgtcatggg ctgcgccggc
     1861 atggcgcggc tgcgccggcc gctggaggac gcgctcggca ttccggtgat cgatcccacg
     1921 caggctgccg tggccatggc tttgggggcg gtgcagtttt cgcgtcacta ggtggtgttt
     1981 catcgaatgg ggcttcgccc aagcctcacg tttccggcgg tgggaccgca ccggcacggc
     2041 tggtaatcag gccgtaatgc tcgatgcgac ggtggcgggc gaagtcgaag atggttttct
     2101 tcccgaagct ggtcgcgtcg agatcgcaag gatggaccag aacctcgtcg ccctcggtgc
     2161 tggcctccgc gacaatctcg ccgttgggat cgacgatcag gctgcctccg atgagtggga
     2221 agccgtcctc gacgccggct ttggcgacag ccacgaccca cgtcgcgttc tgatacgcgc
     2281 cggactgcac cgacagacga ttgtggaaca gccgcttctc gggaccctcc tcgcttttct
     2341 gggagttgac cgcgggcgtg ttgtagccga gcaggaccat ctccacgcct tgcaggccca
     2401 tgacgcgata ggtctccggc cagcgccggt cgttgcagat cgccatgccg atgatgccgc
     2461 ccatcgcccg ccacacatcg aagccgatat cacccggctc gaaataacgc ttttcaaggt
     2521 gctggaaggc gcgctcggtg tcgaattccg aatggcccgg cagatgaacc ttgcgatact
     2581 tgccgacgat tttcgccgac ttgtcggtga ggatgcaggt attgaaatga tgtccgtcag
     2641 gcgtcagttc cgcatagccg aagctcatcg ccatctgata ttccgcagcg cgcgcgaaca
     2701 gcggcattga cgcggtgttc ggcatctcgc gctcaaacca ggtatcgacc tcggcttgat
     2761 cttccatata ccagcgtgga aaaaatgtgg tgagtgcgag ctccggataa acgatcaggt
     2821 ctgctccgcc ggccctggcc tcgtccatca gagcgagcat gcgcttaacg acgacttccc
     2881 ggctatcggc gcgctggatc ggccccatct gagcggcagc gacattgatg actcgcatag
     2941 ggaacttcct ggctgcttta atttcacgct gcagtggaca aaaaaagagc agtcgagcgc
     3001 tgcgcctttc gcagtccatt tccaaattcg atttcaccat tagaattccg attgccgcga
     3061 agtcaattcc gttcagcgac ggccggtaaa acggttctgc gtgtcacgtt ggctgagatc
     3121 gcgtcgcgat agtctaaaag actgacaacc agcgcgacat gcccaaaggc tcgctttttc
     3181 atctgcgaac tcgaagcgcg acctaaagtt gtgatgcgcg agatccgcat tttaatcatc
     3241 tgccattatc gccagagcac ggcacgaacg gacgcaacat ccacgacaaa gaagttagca
     3301 gccaaggcgt ccggggttcg aatcgccgtt gtgttcagtc gcgattcgac tatacttggg
     3361 cgttcggctg cggccgcact actaatatta tcgttggtga ccaggcccgt ctgcttaggc
     3421 gggccgtcgc catttaaggg tcgatggcga tgactggttc caatcgggtt gaccatattc
     3481 gtattacctc gcacccggca ccgggtgcca agcacgacta cccgatcgac tggggcgcag
     3541 ccagcgcgcg cgaacgcgga ccggtgattg gcacggtctc gcgcccacag gatcgcaacg
     3601 tcatcggtac ccatggcggc tcctattcgg tttatcgcgc gctcgccgtc tcttcgggcg
     3661 cactcgatcc gatccggcgc ccggacctca ccaacaccca tcccgccgag acgatcgggc
     3721 cgttttcgca atgggccgat ccgcagcgga tcgtttcgct cgatccgtgg ggccatctca
     3781 tcgcggaaaa cttcgcgaaa gaaatcgccg aaggcatcga tatccgtccg agcattgccg
     3841 tcactcgcgc gcgccttgat ctgcccgaac tgcaggcggc gatcgaagcc ggccggctca
     3901 agcatgacgg cgaggtcgtg cacaagaatg gaagcgtctc cgtggtcaag atcgcgatcg
     3961 acccggtatg gtatctgccg ggcatggcgg cgcgcttcgc aaccaccgag aataatctgc
     4021 gccggcaatt gttcgagcag accgcgggca tgtttcccga actggtgacg cggcctgatc
     4081 tgcaggtttt tctgccgccg atcggcggca cgaccgccta tctgttcggc gatgttacca
     4141 aactgcctga ccaccgcacc aggataacct gccgcgtgca tgacgaatgc aacggctccg
     4201 acgtgtttgg ctcggacatc tgcacttgcc gtccatacct gctccacggc atcgaggaat
     4261 gcgcgcgcgc cgggcagacc ggcggactcg gcatcatcat ctataatcgc aaggaaggcc
     4321 gcgcgctcgg cgaggtcaca aaatttcttg tctacaacgc gcgcaagcgt caggaaggcg
     4381 gcgatgccgc ggcccagtat ttcgagcgca ccgaatgtgt cgccggagtg caggatgccc
     4441 gcttccagca attgatgccg gacgtggtgc actggctcgg cctgaagcga atcgaccgct
     4501 tcgtttccat gagtgatatg aagcacgacg cgctgaccgg ccagggaatc gaaatcgtcg
     4561 aacgcgtgcc aattcccgac gatctcgtgc ccgccgacgc gcatgtggaa attgcggcaa
     4621 agaaggccgc cggttactac acgtccgagc cggagcaaaa cccggtcaac ccggtcggcc
     4681 gttcgctcga aaagtattga gaggggcggc agtgtccctt gcaaagtccc ttgcaaatct
     4741 cgagacgtca g
//
LOCUS       DATNOI010000551         7787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_587106,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000551 DATNOI010000000
VERSION     DATNOI010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7787
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587106"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..735
                     /locus_tag="VN968_22235"
     CDS             61..735
                     /locus_tag="VN968_22235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXL31989.1"
                     /translation="MPINADYRGPGDLPEVIPVFPLPGALLLPRGQMPLNIFEPRYLA
                     MVDDALRDGHRLIGMIQPDVSHSQNEPRPALFRVGCVGRITQLAEAGDGRYILELTGI
                     ARFKVVEEISALTAYRQCKVDYFPYIDDFTARKGEEAVDREALLEVLTDFLKANNLKV
                     DWEGVESAPNEALVNALAMMSPYGPPEKQAMLEAPDLKTRAEILIAVTEMDLAKKRTS
                     GDTGLQ"
     gene            788..1378
                     /locus_tag="VN968_22240"
     CDS             788..1378
                     /locus_tag="VN968_22240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXL31990.1"
                     /translation="MTTVHIRYLTHPQVKIEPAVPVPSWGLSDVGRIRTEALANAGWL
                     SETTQIISSGERKAIETAAIIAGQLKVTVEVRDAMHENDRSTTGFLAPDEFETVADQF
                     FAHPLVSIRGWERAIDAQLRIVREVERVLARNRSGDVLFVGHGAVGTLLFCHYSGLAI
                     DRTHDQPAGGGHCFTLVKDGRRVLHPWRRMEDLPRA"
     gene            complement(2074..2412)
                     /locus_tag="VN968_22245"
     CDS             complement(2074..2412)
                     /locus_tag="VN968_22245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HXL31991.1"
                     /translation="MTMKKLVLAALCALAALAGSPSQAQQATEVHVTYSKGVFQPSEL
                     RAPPDKPIAIRVKNLDAKAMEFESTSLRVEKVVAANSEGVINVRALKPGRYEFYDDFN
                     AKARGALTVQ"
     gene            complement(2457..3356)
                     /locus_tag="VN968_22250"
     CDS             complement(2457..3356)
                     /locus_tag="VN968_22250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31992.1"
                     /translation="MRAAIQLGRRHALALGAIGGLFWCSGAARADDASSWRDLETKYI
                     FGFTTGSGIGLEGEKEVTTDTIGRFGKRDGHYAATETKYEFEFTPSQFIQIEFGALGS
                     THHIGGVTDLDDRSQIALGGAFAELRYLAIERTSNNPLSVTLAVEPTVRRIDETSGER
                     VRNFEFETTVNADLELLKNRLFAGVNLLYEPEVTQTATGDTQREATFGGSAALSLRVA
                     SNVVLGGEVWYLRHYDAANLTAFTGDAVMLGPSLYIRFTPKMFMTAAWNTQVWGREVG
                     NPVSLNLSEFQRNRAKLKFALEF"
     gene            3700..5019
                     /locus_tag="VN968_22255"
     CDS             3700..5019
                     /locus_tag="VN968_22255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HXL31993.1"
                     /translation="MAACLVVAGTRVYGALAQSGLLVGTAAFGDWRGDKPGVSRLIRP
                     EDLPRPGATPSSANVSRVVPRPSAAVPQVPARFKVELFAEGLSGPRQMRVAPNGDIFV
                     AETSAGRIRILRAADGDSRPSANETYANGLNQPFGIAFFPSGSNPEWIYVANTDSVVR
                     FPYRAGDTKAKGKPETVVSRLPHGYGHSTRDIVFTNDNRRMLVSVGSQGNDGEGIGSP
                     PGGLQSWSDEHPLGASWASETDRAAVLAFDPDGKRTGVFATGIRNCVGLAVHTTSGDV
                     YCSTNERDGMGDNLVPDYVTRVREGAFYGWPWFYIGSNEDPRHAGERPDLKGKVTVPD
                     VLIQAHSASLGLTFYNGTAFPTEYRGDGFAAEHGSWNRSKRTGYKVIRIRLKDGVPTG
                     EYQDFVTGFVINDSEVWGRPVGVAVAHDGALLVSEDGNGTIWRITPQ"
     gene            complement(5116..5466)
                     /locus_tag="VN968_22260"
     CDS             complement(5116..5466)
                     /locus_tag="VN968_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL31994.1"
                     /translation="MRDVLLQCAGIAAIAVALIHGVLGETKVFARATIEPPRLRNLIR
                     LVWQAGTVAWIGGGVLLIAAPWMASESARHWIVATLAAVFGFAALANAWALRGRHFGW
                     VALSAVVALAVAGY"
     gene            5565..6101
                     /locus_tag="VN968_22265"
     CDS             5565..6101
                     /locus_tag="VN968_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL31995.1"
                     /translation="MADQLSAKDWLDQGLRALARSGFTALKAEPLAKAMGVSRGSFYW
                     HFADIGAYHAAILKHWREVAAEQIIAGLEAVSGDDNPLQLLLRRAFGTKPALENAFRT
                     WATVDPAARSAVQAIDRRRLGYVEKLLGASGLPADVARPRAQILYWAFLGFALSDRPL
                     PQARQAEVIDELLRMASR"
     gene            6169..6366
                     /locus_tag="VN968_22270"
     CDS             6169..6366
                     /locus_tag="VN968_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HXL31996.1"
                     /translation="MTSSPERLEGTVDPKLLEILVCPLTKGPLEFDATRQELISRSAK
                     LAYPIRDGIPIMLPEEARKID"
     gene            6371..7306
                     /locus_tag="VN968_22275"
     CDS             6371..7306
                     /locus_tag="VN968_22275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL31997.1"
                     /translation="MRARAIIGALAILNFLGMTLNASADETKIRVGALDAVLTVPADV
                     ERPPVALLLAGSGSTDHDGNGPRIKPATLKKLSEQLALRNIATLRYDKRGAGGWKAEF
                     GPPEDFRFKDYVDDAAALVNYLRGSGKFSRVIMVGHSEGGLVAILTARRVPVDRVVLL
                     ATAARRQGDLLKAALEKALDPDLFKPIAKAIDDIMAGQIVDPPPGLAIPPSMQPGFAS
                     AFAEDPIDPLKLIEQPTLIVAGGRDRQVARLDFVALGAASPVAKTLWLPDMNHVLVDV
                     ADETDDVAAYDQPERALDTVLIDSVADFVLANGVR"
     gene            complement(7355..>7787)
                     /locus_tag="VN968_22280"
     CDS             complement(7355..>7787)
                     /locus_tag="VN968_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXL31998.1"
                     /translation="LDKPRAFPLGYFVARSFIGERMALIGDAAHVIHPIAGQGLNMGL
                     KDVAALAEVVVDAARLGMDLGQADVLDRYQRWRRFDTMAMGLATNSLNLLFSNKSTVL
                     RTARDIGLGLVDRAPPLKSLFIRQAAGLAGEVPRLLKGEAL"
BASE COUNT         1463 a         2419 c         2461 g         1444 t
ORIGIN      
        1 cggttttgcg attaggatgc gcgcaagttt agaaactctg gattagcgcc ggaaccgcac
       61 atgccgatca atgccgatta tcgcgggccc ggcgaccttc ccgaggtgat cccggtgttt
      121 ccgctgcccg gcgcgctgct gctgccgcgc ggccagatgc cgctcaacat cttcgagccg
      181 cgctatctgg cgatggtgga cgatgcactg cgcgacggcc atcggctgat cggaatgatc
      241 cagccggatg tttcccactc gcagaacgag cccagaccgg cgctgtttcg cgtcggctgc
      301 gtcgggcgca tcacccagct cgccgaggct ggcgacggcc gctacattct cgaactcaca
      361 ggcatcgcgc gcttcaaggt ggtcgaggaa atctcggcgc tgaccgcgta tcggcaatgc
      421 aaggtggatt atttccccta tatcgacgat ttcaccgcgc gcaagggcga agaagcggtg
      481 gaccgcgagg cactgctcga ggtgctgacc gattttctca aggccaacaa tctcaaggtc
      541 gactgggaag gcgtcgagag cgcgcccaac gaggcgctgg tcaatgcgct ggctatgatg
      601 tcgccctatg gcccgccgga aaagcaggcg atgcttgagg cgcccgatct caagacccgt
      661 gccgaaattc tgatcgcggt caccgagatg gacctggcca agaaacgcac cagcggcgat
      721 acggggttgc agtgatcgac gcaggcgatc tcggcgtcca tcccgcgacc attgccgcat
      781 catccagatg acgaccgtcc atatccgcta ccttacccac ccgcaggtaa agatagagcc
      841 ggctgtccct gttccgtcat ggggcttgag cgatgttgga agaatacgca ccgaagcgct
      901 ggcgaacgcc ggatggcttt cggagacaac gcagatcatc tccagcggcg agcgaaaggc
      961 cattgagacc gccgcgatca ttgcaggaca attgaaagtc accgtcgaag tgcgggacgc
     1021 gatgcacgag aatgatcgat caaccacggg tttcctagcc cccgacgagt tcgagacggt
     1081 ggccgatcag tttttcgcac atccccttgt cagcatccgg ggatgggagc gggcgatcga
     1141 tgcccaattg cgcattgtcc gcgaggtcga acgcgtgctg gcccgaaatc gatcgggtga
     1201 cgtactgttc gtcggccacg gtgccgtcgg tacccttttg ttctgtcact attccggctt
     1261 ggcgattgat cggacccatg atcaacccgc cggcggcggc cattgcttca cgttggtcaa
     1321 ggatgggcga cgcgtgctgc atccatggcg tcggatggag gacttgcctc gcgcgtaagg
     1381 cgtcggctgc gtttccgggg gcggacccgg atgttgcgga gcttaaaggc aatacttcgg
     1441 cttgcggcgt cgacgtggtt gcggagccac agtctgaccg aaaagatcct ccgagtgagg
     1501 gcttaagcac gagagggtgc ggacggaggt tcttttccag cctaggctac atcgatgccg
     1561 tgacaaaatt cgcgggggcg cgacaggtcc ggcaggttgc cagcgtcgcg aaggatggag
     1621 tgggggctct atccgagatg tagccgatgc tttcgatcga tgatgtgtgc cgctcgttgt
     1681 gcggccgttc gtgatcgctt cggtgtttgg aagattggct tccgtgtgat ttgaaattcg
     1741 gcgtcgtgcc ctgagcggcc agcaaccgtg ctgatagagg gtcggccgcg tcgccgtccg
     1801 gtgcccgcgt tgggcgttcc tcccgaactg tccggcggcc ccgagccgcc ggacactttt
     1861 ttcgtcctcg cttcgtggcc aacgttagag gcccaacctg ccgcgctttg gtagcagttc
     1921 cgcgggacca gcgatcgccg agcatcgcat ccgggcccga tgccgatggg tccaaataca
     1981 atcgaaggcg attgtcgaaa caggtaacga gaaacccagg gcaggggatg cagtggttgt
     2041 tcgcgcgtcc acgcgcgaac aaccggccgt cgcctattgc acggtcaggg cgccgcgcgc
     2101 cttagcgttg aagtcgtcgt aaaattcgta gcgtccgggt ttcagcgccc gcacattgat
     2161 gacgccttcg ctgttggcgg cgaccacctt ttcgacccgc agcgaggtgc tttcaaactc
     2221 catggccttg gcgtccaggt tcttgacccg gatcgcgatg ggcttgtcgg gcggcgcgcg
     2281 cagctcgctt ggctgaaaca ctcccttgga ataggtgacg tgaacctccg tcgcctgttg
     2341 cgcctgggaa ggggaccccg cgagcgctgc aagggcgcaa agcgctgcca gaaccagctt
     2401 tttcatcgtc atcggcacag cagacacctg tcgccccttc cgcaggttga ggaagatcaa
     2461 aattccaaag cgaatttgag tttggcgcga ttgcgctgaa actccgatag attgagtgag
     2521 acgggattgc cgacctcgcg cccccaaacc tgggtgttcc acgcggcggt catgaacatt
     2581 ttcggtgtga accggatata gagcgacggc cccagcatca cggcgtcgcc ggtgaaagcc
     2641 gtcagattcg cggcgtcgta atgcctgaga taccagacct caccgcccag gacgacgttc
     2701 gatgcgaccc gaagcgacag cgccgccgaa ccgccaaacg tggcttcgcg ctgggtgtcg
     2761 cctgtcgcgg tctgcgtgac ctcgggctcg taaagcaaat tgaccccggc aaacagccgg
     2821 tttttcagca gttcaagatc ggcgttgacg gtggtttcga attcgaaatt gcgcacgcgc
     2881 tcgccgctgg tctcgtcaat ccgccgtacc gtcggctcga ccgccagcgt caccgaaagc
     2941 ggattgttcg aggtccgctc gatcgcaaga tagcgcaatt ccgcaaaagc gccgcccaat
     3001 gcgatctggc tccgatcgtc gagatcggtg acgccaccga tatgatgcgt cgatcccagc
     3061 gcgccaaact cgatctggat gaactgggac ggcgtaaatt caaactcgta ttttgtctcg
     3121 gtcgcggcgt agtgaccatc gcgcttgccg aaccggccga tggtgtcggt cgtgacttcc
     3181 ttctcgccct cgaggccaat gccggagccg gtggtaaatc caaaaatata cttggtctcg
     3241 aggtcgcgcc aggagcttgc gtcgtcggca cgcgcggcac cgctgcacca gaacaaaccg
     3301 ccgatcgcgc caagcgcaag ggcgtgacgc cgtccaagct gtatcgccgc gcgcatcttg
     3361 ttctcgcatc cgccgtatgt cgggatagat ggcatcagct agctgctagc tgcggcacag
     3421 gcaaaaagac tcgttctaaa ctagagacat tctaatagtt gggtgtgttg accaagctcg
     3481 ccgacgccac gcccgctgct tcaagcagac gatcacttgg ccgccggcgt cattcgcgat
     3541 ttcgacatgt tgatcgagga aggatttccc ggcggctcgg ttgcccgaac ggtggcgcta
     3601 cttttgcaag tggcccatgc aacgtgaacg tcgtactttc ggcatatgat ggctgaggat
     3661 ccagagataa tgcatttaaa cggtcgcgca tcatattgga tggcagcatg cctggtggtc
     3721 gcaggcaccc gcgtttacgg cgcgctggcc caatcagggc tgctggtagg caccgccgcg
     3781 ttcggcgact ggcgcggcga caagcccggc gtcagccgcc tgataagacc tgaagatctg
     3841 ccgcggcccg gggcaacgcc atcgtccgcc aacgtgtctc gcgtggtccc gcgaccttcg
     3901 gccgcggtcc cgcaagttcc tgcgagattt aaggttgagt tgttcgccga gggactgagc
     3961 gggccgcgcc agatgcgggt ggcccctaac ggcgacatct tcgtcgcaga aaccagcgcg
     4021 ggacgaattc gtatcttgcg ggcggccgat ggcgactcaa ggccttccgc gaacgagacc
     4081 tacgcgaacg ggcttaacca gccgttcggg atcgcttttt tcccgagtgg aagcaatccg
     4141 gagtggattt acgtcgccaa caccgacagc gtcgtccgct tcccctacag ggcgggcgac
     4201 accaaagcca aaggcaaacc tgaaaccgtc gtctccagat tgccgcatgg ctacgggcac
     4261 tcgaccaggg acattgtctt caccaacgac aacaggcgga tgctggtatc ggtcggttcg
     4321 caaggcaatg acggagaggg tatcggaagc ccgccgggag gattgcaatc gtggagcgat
     4381 gaacatccgc tcggcgcaag ctgggcaagc gagaccgacc gcgccgccgt gctggcgttc
     4441 gatcccgatg gaaaacgcac cggtgtcttc gcgaccggta tccgtaattg tgtgggtctc
     4501 gccgtgcaca ccacgagcgg cgatgtgtat tgctccacca atgagcgtga cgggatggga
     4561 gacaatctgg tgccggacta cgttacccgg gtgcgcgaag gggcgttcta cggctggccc
     4621 tggttctata tcggcagcaa cgaagacccg cggcacgccg gcgaacgtcc cgacctgaag
     4681 ggcaaggtca ccgttcccga cgttctcatt caggcgcact cggcgtcgct tgggctgaca
     4741 ttctacaacg gcactgcgtt tccaacggaa tatcgcggtg atgggttcgc agccgagcac
     4801 ggctcgtgga accggtccaa acgcacgggc tataaggtta tccgcattcg cctcaaggac
     4861 ggagtgccga ccggcgagta ccaggacttt gtcaccggct ttgtcatcaa cgattccgag
     4921 gtatgggggc ggcccgttgg cgtagccgtc gcgcatgacg gcgcattgct ggtgtcggaa
     4981 gacggcaacg gcacgatctg gcggattacg cctcaatgag gataggtcgg cgcatccgtc
     5041 accgtacggt cgggcaatct gtccacgctc gcccactctc gtgacgcatt gacggcggtt
     5101 tcatgaggcc gcgctctaat accccgccac ggccagcgcg accaccgcgc tcagcgccac
     5161 ccatccgaaa tgccggccgc gcagcgccca ggcattggcc agcgcggcaa acccaaatac
     5221 cgcggcgagg gtcgcgacga tccagtgccg cgccgactcc gacgccatcc agggagcggc
     5281 gatcagcagc acgccgccgc cgatccatgc cacggtgccg gcctgccaga ccagacggat
     5341 cagattgcgc agcctcggag gctcgatggt ggcgcgggca aacacctttg tttcgcccag
     5401 cacaccgtgg atcagcgcta ccgcgatcgc ggcgatccca gcgcactgca acagaacatc
     5461 gcgcatcgga tgcgtcctcc atacatgact gtatggtagc tatggcggag gcgctggtgc
     5521 cggtcaatac ggtagtgtat ggtcacgttg tcgcagggat ccggatggcc gatcaacttt
     5581 ccgcaaagga ctggctcgat cagggcctca gggcgctggc gagaagcggt ttcacggcgc
     5641 tcaaggccga gccgctggcg aaagcgatgg gcgtctcgcg cggcagcttc tactggcact
     5701 tcgccgacat cggcgcctac cacgccgcca tcctcaagca ttggcgcgag gtcgccgccg
     5761 agcagatcat cgccggcctc gaggccgtgt ctggcgacga caatccgctg cagctcttgc
     5821 tgcgccgggc attcgggacc aagccggcgc tggagaacgc gttccgcacc tgggctacgg
     5881 tcgatcccgc ggcgcgaagc gccgtgcagg ccatcgaccg ccgccggctc ggctatgtgg
     5941 agaaactcct tggcgcgtcc ggattaccgg cggatgtggc ccgtccccgg gcgcaaattc
     6001 tttactgggc gtttctcggc tttgccctgt cggacaggcc gttgccgcag gcgcggcaag
     6061 ccgaggtgat cgacgagctg ctccggatgg cctcgcggtg agggcgggcg gctacacgtc
     6121 gtgatcgaat atgctacagc gggtcgaatt gccaagtggg agcagtgcat gacgtcctca
     6181 cccgaacgcc tggaaggcac cgtggatccg aaattgctcg aaattctggt ctgcccgctg
     6241 accaagggcc cgctggagtt tgacgccacc aggcaggagc tgatctcgcg ttccgcaaag
     6301 ctggcctatc cgatccgcga cggcattccg atcatgctgc cggaagaggc acggaagatt
     6361 gattagcccg atgcgcgccc gggcgatcat tggtgcgctc gcgatcctca acttcctcgg
     6421 catgaccctg aacgcatccg ccgacgaaac caagatccgc gtcggcgccc tcgacgcggt
     6481 gctgaccgtt ccggccgatg tggaacggcc gccagtcgcg cttttgctcg caggctccgg
     6541 atccaccgac catgacggca acggaccgcg gatcaaaccg gcaaccctga agaaactctc
     6601 cgagcaactc gcgctccgca atatcgcgac gcttcgctac gacaagcgcg gcgcgggcgg
     6661 atggaaagcg gaattcggac cgccggaaga ttttcgtttc aaggactatg tcgacgatgc
     6721 cgcggcgctg gtgaattatc tgcgcggcag cggaaaattt tcgagggtta ttatggtcgg
     6781 acacagcgag ggcggcctgg tcgcaatcct cacggcccgg cgcgtgcccg tcgaccgtgt
     6841 cgtcttgctg gccaccgcgg cgcggcggca gggcgacctg ctcaaggccg cgcttgaaaa
     6901 ggcgcttgat ccggacctgt tcaagccgat cgcgaaggcg atcgacgata tcatggccgg
     6961 gcaaattgtc gatccgccgc cgggactggc gatcccgcca tcgatgcagc ccggcttcgc
     7021 ctcggccttc gcggaagacc cgatcgaccc cttgaaactg atcgaacagc cgaccctgat
     7081 tgtcgcgggc ggccgcgacc ggcaggtggc acggctggat ttcgttgccc tcggcgcagc
     7141 gtcaccggtg gcgaaaacgc tttggcttcc ggacatgaat cacgttctgg tcgatgttgc
     7201 tgacgaaacg gatgatgtgg ccgcctatga ccagcccgaa cgcgcactcg acactgtcct
     7261 gatcgattcg gtcgcggatt ttgttcttgc gaacggcgtc cgttgatctt ggctggcgtc
     7321 gtcccggcgc acgccggaac gacgctgatg attgctacag cgcctcgccc ttcaacagcc
     7381 gcggcacctc gccggcaagc cccgccgcct gacggatgaa caggctcttc agcggcggcg
     7441 cgcggtcgac cagcccgagc ccgatatcgc gcgccgtgcg caaaaccgtc gatttgttcg
     7501 aaaacagcag attcaacgaa ttggtggcga gccccatggc catggtgtcg aagcgtcgcc
     7561 agcgctgata gcggtcgagc acgtcggcct gcccgagatc catcccgagc cgcgcggcat
     7621 cgaccaccac ttcggcgagt gcggcgacgt ccttcaggcc catgttgagg ccttggcctg
     7681 cgatcgggtg aatcacatgc gcggcgtcgc cgatcagcgc catccgttcg ccgatgaacg
     7741 agcgcgccac gaaatagccg agtggaaacg cgcgcggttt gtcgagc
//
LOCUS       DATNOI010000552         2932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2197951,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000552 DATNOI010000000
VERSION     DATNOI010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2197951"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..348
                     /locus_tag="VN968_22285"
                     /pseudo
     CDS             145..348
                     /locus_tag="VN968_22285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            466..1497
                     /locus_tag="VN968_22290"
     CDS             466..1497
                     /locus_tag="VN968_22290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXL31999.1"
                     /translation="MRHYAGLDVSVKETSVCIVDESGKVCRQTKVPSHPEDLARALHD
                     RAWCLERVGLEAGPLSQWLFSGLAATGLPVVCIETRHTKAFLQAQVNKSDRNDARGIA
                     QMMRVNLFRPVHVKTLTSQKRRALLTARKLLQEKALAVDNDIRGLLRNFGLKVGPIGA
                     SRFETRTRELVDGMAELAEIIDPLLEARRKLRELFAVLHRKLLVIVRDDTVCRRLMTV
                     PGVGPVVSMAFTATIDVPARFKSSKAVGAALGLTPILNQSGESKRLGRVSLCGDGMTR
                     TLLYEAAQVMLTRTTKWSWLKAWAMKVAARRGIQKAIVALARRLAVILHRIWVSGTDF
                     RWTREVQSP"
     gene            1769..2926
                     /locus_tag="VN968_22295"
     CDS             1769..2926
                     /locus_tag="VN968_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HXL32000.1"
                     /translation="MTSVGRIEKHMHDLSDEVLCARWVENPYYQFFCGELSFCHQLPF
                     DRSSLTHWRQRLGEEQLVALLQESLSVAHKTGALATRDLERVVVDTTVQPKAIAHPTD
                     ARLCHRALQKLVDLAERTGVRLRQSYRRVAKRAAIMVGRYTHAHQFNRARRELKFLRI
                     RLGRVIRDIRRKIGDNETLRERFAQLLALAVRVRFQDHRQRGPKVYSLHAPEVECIGK
                     GKARAPYEFGCKVSIATPATKPKGGQFVLHAKALHGNPFDGHTLGRVITELEQQTGVE
                     TRRIHVDKGYRGHNHKEKFRVWISGQVRGVTKPIRREMRRRAAVEPVIGHTKAEHRMG
                     RNYLKGRDGDRINAVLAAAGYNFGLLLRWLGGLLRALLQALFTALSDPQPA"
BASE COUNT          557 a          847 c          982 g          546 t
ORIGIN      
        1 tgaacggtgt agcggaacgg cggagctgaa gtagtcgaat ttgcaagaaa cggcgtgttt
       61 gtggtcggtt ttctggcgat ttgggattcc ggttttcgct aatctgtgcg attcggtggc
      121 gggtcatcgg cgtgggggga cgctatggcg aagccgcggg acaatcgtca gaaggatttg
      181 ctacggccgg cgctggagga gatcgtcgac ctcggtcatc cgctggtgcg gttggcacgc
      241 gagatcgatt gggggtttct cgacagccgg tttgccggcg tgtgtgcgtc aggtgcgggc
      301 cagccgccat tgccgacgcg cctggtggcg gggctgttca tcttgaagat ggagtattcg
      361 gccggccggt gtccctcact caaggtgagg tgctgcgctt cgggctgatg gtagacgtgg
      421 tctgcgttta ggggcagcca caaacatcag aaggagaaac gcagcatgag gcactatgcc
      481 ggtctggatg tctccgtcaa agagacgtcc gtgtgcatcg tcgacgagag cggcaaggtc
      541 tgccggcaga cgaaggtgcc gagccatccc gaggatctcg cgcgggccct tcacgaccgc
      601 gcctggtgtt tggagcgagt cggtctcgag gccgggccat tgtcgcaatg gctgttcagc
      661 ggcttggcgg caacagggct accggtggtg tgcatcgaga cgcggcacac gaaagccttc
      721 ctccaggcgc aggtcaacaa gagcgaccgg aatgacgcac gcggcatcgc gcagatgatg
      781 cgcgtcaacc tgttccggcc ggtgcacgta aagacgctga ccagccagaa gcgccgcgcg
      841 ctgctgactg cacgcaagct gctgcaggaa aaggcgctcg ctgtcgataa cgatatccgc
      901 ggcctgctac ggaacttcgg gctcaaggtt ggaccgatcg gtgcatccag gttcgagacg
      961 cgcacacgcg agctcgtcga cggcatggcg gaactcgccg agataatcga tcctcttctc
     1021 gaggcccgcc ggaagctgcg cgaactgttt gccgtgcttc accgcaagct gttggtgatc
     1081 gtgcgggatg acacggtgtg ccggcgtttg atgacggtcc caggagtcgg tccggtagta
     1141 tccatggcct tcaccgccac catcgacgtt ccggcccgct tcaagagctc caaggctgtc
     1201 ggggcggccc ttggattgac cccgatcctt aatcaatccg gcgaaagcaa acgcctcggc
     1261 agggtctcgc tgtgtggcga tggaatgacg cggacgcttc tctatgaggc agctcaggta
     1321 atgctgacac ggacgaccaa atggtcgtgg ctgaaagcat gggcaatgaa agtggcggcg
     1381 cgtcgcggga tacagaaggc aatcgtcgcc ttggcccggc ggctggcggt catcctgcat
     1441 cgcatctggg tgagcggcac ggatttccgc tggacaaggg aagtgcaatc gccttaacgc
     1501 gaacagtccc gtaatagagg agcggaccag gaggagaagt ttgcggttcc gcgttgaggc
     1561 ggagagttgt ccctcgcggg acgagggacg aagtgagttc ggtgcatgtg ctgttccttg
     1621 agcttcgtga gcacaaagga cgccttgaag attgttccac ttcgttcttc tgatcccatc
     1681 atggaagggc ctatggggcc gattccggag agaagcgaga acccggaggg ataggtccac
     1741 gtcacaacgc aagacggtcc gaaagcgctt gacctcggtt ggccgaatag agaagcacat
     1801 gcatgaccta tcggacgagg tgctgtgcgc gcgctgggtt gaaaacccct attatcagtt
     1861 cttctgcggt gaattgagct tctgtcacca gctgccgttc gatcgctcct cgctgacgca
     1921 ttggcgccag cggctgggcg aagagcagtt ggtggctttg ctccaggaga gcttgtcggt
     1981 ggcgcacaag accggcgcct tagcgacgcg ggatcttgag cgcgtggtgg tcgacaccac
     2041 ggtgcagccg aaggcgatcg cgcacccgac tgacgcgcgg ctatgccatc gcgcgctgca
     2101 aaagctggtc gatctcgccg agcgcactgg agtgcgactg cggcagagct atcgtcgggt
     2161 ggccaagcgc gccgcgatca tggtcggccg ctatacccat gcccaccagt tcaatcgggc
     2221 gcggcgagaa ctcaagttcc tgcgcattcg gctcggtcgg gtgatccgcg atatccgccg
     2281 caagatcggc gacaacgaga cgctgaggga gcgcttcgcc cagcttctcg ccttggcggt
     2341 gcgggtgcgc tttcaggatc accgccagcg cggtcccaag gtctattcgc tgcatgcccc
     2401 cgaggtcgag tgcatcggca aaggcaaagc tcgggcgccc tatgagttcg gctgcaaagt
     2461 gtcgatcgcc accccggcga caaaacccaa aggcggccag ttcgtgctgc acgccaaggc
     2521 gctgcatggc aacccgtttg acggccatac gctgggacgt gtcatcaccg aactggagca
     2581 gcagaccggc gtcgagaccc gccgcatcca tgtcgacaag gggtaccgcg gtcacaatca
     2641 caaagagaag ttccgggtgt ggatcagcgg ccaagtgcgc ggggtcacga aaccgatccg
     2701 tcgcgagatg aggcgccgcg ccgccgtcga accggtcatc ggccatacca aggccgagca
     2761 ccgcatgggc cgcaattatc tcaagggacg cgacggcgac cgcatcaatg ctgtgctcgc
     2821 cgccgccggc tacaactttg gcctcctcct gcgctggctc ggagggcttt tgcgcgccct
     2881 gttgcaggcg ctcttcacag cgctctcgga tccacaaccg gcctaaaacc gg
//
LOCUS       DATNOI010000553         3945 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_587325,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000553 DATNOI010000000
VERSION     DATNOI010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587325"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1330)
                     /locus_tag="VN968_22300"
     CDS             complement(38..1330)
                     /locus_tag="VN968_22300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL32001.1"
                     /translation="MVDLAPQVAAQADASARPSARRYYVLGLLTVVYALNFLDRTIFN
                     VLIEPIKKEFTLSDTTMGLLAGFGFVLFYSLLGIPIARVADRLNRRNIVALAFAFWSA
                     MTFLCGMAQSVATLALARIGVGIGESAGTPASQSMVADLFDKNERPRALGVYAIGTYL
                     GVFLGYFIGGYVNQHYGWRMAFFTAGLPGIALAAVLWLTVSEPARGAMAETFTPEPIG
                     PTVGFLASQRTFVIVLIGFCLTTYTNYATAAWIPPFLARVHHLSSAEIGTYAGTFKGL
                     FGMAGTLVGGLMVAQISRRDDRWKLWAPAITSGLAGPVFAVCMLTSDFATMVAALALT
                     SFLVGFHLGPIFAIAQTVARPSMRALASAIILLTATCFGQGVGPLAVGMINDALKNDY
                     GVNAVRYSLLSAAVTTTLGALLFVWAARSIRADIKRAT"
     gene            complement(1371..2561)
                     /locus_tag="VN968_22305"
     CDS             complement(1371..2561)
                     /locus_tag="VN968_22305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXL32002.1"
                     /translation="MNITQRDRDLGTAYAMRPSASRTELTEVGRGTPMGELLRRYWHP
                     IGLVADATDILRKVRALGEDLILFRDGHGRVGLLHARCCHRGTTLYYGKVEEDGIRCC
                     YHGWKFDTEGRCLEQPCEPDGGQFRDKVRQPWYPVAERYGLIFAYMGPSEKMPVLPRY
                     EVLEKMDDGEFVEADDSSIGGGGPAVIPCNWLQHFENVVDPYHVPVLHGSFSGPQFTN
                     MMASMPQVKFEMSPRGVTVRSIRNQDDGKIFYRVTEAVLPTLRVVPNPRVAQFARVES
                     IGWTLPIDDTSFRIYVAGRVKQPGDIGRMRSKFNGKFWWDMTEQEHQQFPGDYEAQVG
                     QGPVTLHSEEHFGQSDRGILMIRRMLADQLDAMAAGRDPVGVSFDQNASPVEFEAGNF
                     TRDA"
     gene            2718..3215
                     /locus_tag="VN968_22310"
     CDS             2718..3215
                     /locus_tag="VN968_22310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXL32003.1"
                     /translation="MAVPPPASPDKDDRLAPITAMMSSRLMVLANLLKRGAILRYKRL
                     VGLSSVEFGLVASLGRRPPMSVARLAEAVGMDKGQISRALAGLVSRKLVSRAVNPRDN
                     REVLVCLTRTGLIAHETIVAGALERNQRLLEQLSEDEVAMLLGHIDRLTDTAAKMLDA
                     EKDLS"
     gene            3393..3758
                     /locus_tag="VN968_22315"
     CDS             3393..3758
                     /locus_tag="VN968_22315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXL32004.1"
                     /translation="MTGILQNYLPLVVFIGVAGLIGLALLIAPFLVAFQQPDPEKLSA
                     YECGFNAFDDARMKFDVRFYLVAILFIIFDLEVAFLFPWAVAFGKLGATGFWSMLVFL
                     VVLTIGFAYEWKKGALEWD"
     gene            3749..>3945
                     /locus_tag="VN968_22320"
     CDS             3749..>3945
                     /locus_tag="VN968_22320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HXL32005.1"
                     /translation="MGLSPATAKPGFAAAVAPAATGILDPSTGRPVGATDPYFLEVNH
                     ELSDKGFFVTATDDLITWARTG"
BASE COUNT          746 a         1221 c         1256 g          722 t
ORIGIN      
        1 atcaagggct actaaatagc cgttgtcccc cgcagattca cgtcgcccgc ttgatgtccg
       61 cgcgaatgga cctggccgcc cagacgaaca acagggcgcc aagcgtggtc gtgaccgcgg
      121 ccgaaagcag cgaatagcgc acggcattga cgccgtaatc gttcttcagc gcatcattga
      181 tcatgccgac cgccagcggg ccgacgccct ggccaaaaca ggtcgcggtc aacagaatga
      241 tcgcggaggc cagcgcccgc atgctggggc gggcgacggt ctgcgcgatg gcaaagatcg
      301 gcccgagatg gaatccgacc agaaacgagg tcagcgccaa tgcggcgacc atggtcgcga
      361 agtctgacgt caacatgcac accgcgaaca ccggtccggc gaggcccgac gtgatcgccg
      421 gtgcccacag tttccagcga tcgtcgcggc ggctgatctg cgccaccatc aggccgccga
      481 ccaatgtgcc cgccatcccg aacaggccct tgaaggtgcc ggcataggtt ccgatctcgg
      541 cgctgctcag atgatgcacg cgcgccagaa agggcgggat ccaggccgcg gtggcgtaat
      601 tggtataggt cgtcaggcaa aagccgatca ggacgatcac gaaggtccgt tgcgaggcga
      661 gaaagccgac tgtcggtccg atcggctcag gcgtgaaggt ctccgccatg gcgcctcggg
      721 ccggctccga taccgtcagc cacaacaccg cggccagcgc aatgccgggc agtccggctg
      781 tgaagaacgc cattcgccag ccgtagtgct gattaacgta gccgccgatg aaatagccga
      841 ggaacacccc gagataagtg ccgatggcgt agacgccgag cgcgcggggg cgttcgttct
      901 tgtcgaagag atcggcgacc atggattgcg acgccggggt gccggcggat tcgccgatgc
      961 cgacgccgat tctggcgagc gccagcgttg ccacgctctg cgccatgccg cacaggaacg
     1021 tcatcgcgct ccaaaaggcg aaggcgaggg ccacgatatt gcgccggttg agccggtcgg
     1081 cgacccgcgc gatggggatg ccgagcagcg aatagaacag cacgaagccg aagcccgcga
     1141 gcagccccat ggtggtgtcg ctgagcgtga attccttctt gatcggctcg atcaggacgt
     1201 tgaagatcgt gcggtcgaga aaattcagcg cgtagaccac ggtgagcagc cccagcacgt
     1261 agtagcggcg ggcagatggc ctcgccgatg cgtcggcctg cgcggcgacc tgcggcgcga
     1321 gatcgaccat gtttccccct gagcttcttt ttggaacgag acgccgtcgc tcacgcgtcg
     1381 cgagtgaaat ttccagcctc gaattccacc ggtgacgcgt tttggtcgaa cgagaccccg
     1441 acgggatcgc gcccggcggc catggcgtcg agctgatcag ccagcatccg ccggatcatc
     1501 aggataccgc gatcgctctg gccgaaatgc tcctcggaat gcagcgttac cggtccctgg
     1561 ccgacctgcg cctcgtaatc gccggggaat tgctgatgtt cctgttcggt catgtcccac
     1621 cagaacttgc cgttgaactt cgagcgcatc cgtccgatat cgccgggttg cttgacccgg
     1681 ccagcgacgt agatacggaa cgaggtgtcg tcgatcggca gtgtccagcc gatcgactcg
     1741 acgcgggcga actgcgcgac ccgcggattg ggcaccacgc gcagggtagg aagaacagct
     1801 tcggttacgc ggtagaagat cttgccgtcg tcctggttgc ggatcgaacg cacggtgacg
     1861 ccgcgcggcg acatctcgaa tttcacttgc ggcatcgagg ccatcatgtt ggtgaattgc
     1921 gggccgctga acgatccgtg cagcaccgga acgtggtagg gatcgaccac gttctcgaaa
     1981 tgctgcagcc agttgcaggg aatgacggcc ggtccgccgc cgccgatcga gctgtcgtcg
     2041 gcttccacga attcgccgtc gtccattttc tccaggactt cgtagcgtgg cagcaccggc
     2101 atcttctctg atgggcccat ataggcgaag atcagcccat agcgttcggc aaccgggtac
     2161 cagggctggc gcaccttgtc ccggaactgg ccgccatcgg gttcgcaggg ctgttcaagg
     2221 cagcggcctt cggtatcgaa cttccagccg tgatagcagc agcggatgcc gtcctcctcg
     2281 accttgccat aatagagcgt ggtgccacga tggcagcagc gggcgtgcag caggccgaca
     2341 cggccatgcc cgtcgcggaa caggatcagg tcctcaccca gcgcacgaac ttttcggagg
     2401 atgtcggtgg cgtcagccac aagaccgatc gggtgccagt aacgccggag cagttccccc
     2461 atcggcgtgc cgcgcccgac ctcggtcagt tcggtccggc tggcggacgg cctcatggcg
     2521 taggcggtgc cgaggtcgcg atcgcgctgg gtgatgttca tcgccgcttc ctcccacccg
     2581 gctttgccgc cgtgttcgtt gagattgaga acctttcgga aagtgaatag tagatagatg
     2641 acaatgtcaa cgatcccaaa aaccttggca ccagaagatt ttgttggcac acgtctgcca
     2701 tgaaccagag gtcgaaaatg gcggtcccgc ctccggcgag tcccgacaaa gacgatcggc
     2761 ttgcaccgat cacggcgatg atgtcgtcgc ggttgatggt acttgccaat ctgctgaagc
     2821 gcggcgccat cctgcgctac aaacgtctgg tcggactttc ttcggtggag ttcgggcttg
     2881 tggcgtcgct ggggcgccgg ccgccgatga gcgtggccag gctcgccgag gcggttggca
     2941 tggacaaggg acagatcagc cgggcgctgg ccggactggt atcgcgcaag ctggtttcga
     3001 gagcggtcaa cccgagggat aatcgggaag tgttggtctg cctaactcgg accgggctga
     3061 tcgcccatga gacgatcgtg gcgggtgcac tggagcgcaa tcagcgcttg ctggaacagc
     3121 tcagcgagga cgaagtggcg atgctgctgg ggcatatcga tcgtctcacc gatactgccg
     3181 cgaaaatgct cgacgcggaa aaagacttga gctgagaaaa gctgcgtgcc tcagcttcgg
     3241 gcgtcctccc cgggttatcc cgccgcatct ggggccgggc agggccgtca agatagcttg
     3301 tcttgcgccc cttgttgcgc tgcgctaagc ctcggaataa ttccaatgta acgaatctgt
     3361 ggcggtcaaa accgcaggaa aaggcaccgc cgatgaccgg catcctgcag aattatctac
     3421 cacttgtggt gtttatcggg gtcgcgggcc tgatcggtct ggcgctgctg atcgctccct
     3481 tcctcgtcgc cttccagcag cccgacccag aaaagctttc cgcctatgaa tgcggattca
     3541 acgcttttga cgacgcccgc atgaagttcg acgtccggtt ctacctggtg gcgatcctct
     3601 tcatcatctt cgacctcgaa gtggcgttcc tgttcccgtg ggcggtggcg ttcgggaagc
     3661 tcggggcgac cggcttctgg tcgatgctgg ttttcctcgt cgtcctcacc atcggctttg
     3721 cctacgaatg gaagaaaggc gcgctcgaat gggattgagc cccgctaccg ccaaaccggg
     3781 atttgccgcc gccgtcgcgc cggctgcgac cggcattctc gatccctcca ccggccggcc
     3841 ggtcggcgcc actgacccat actttctcga ggtcaatcac gaactgtccg acaagggctt
     3901 cttcgtcacc gccaccgacg acctgatcac ctgggcgcgc acggg
//
LOCUS       DATNOI010000554         6521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_40817,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000554 DATNOI010000000
VERSION     DATNOI010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40817"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1394)
                     /locus_tag="VN968_22325"
     CDS             complement(84..1394)
                     /locus_tag="VN968_22325"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32006.1"
                     /translation="MTDEAKTQNWGETFWYRGKVTRRRLVGYGSAAAGALGASMLVPA
                     PWQAAFGQAKPYKIGTEQPLSGAGAAGGKTALVGVQMAVDRINKSGGINGRPIELIVA
                     DDESKPDVGRRKVEKLLVEDKVDVHVGGFLSNICLACMPVFEEYKVVNIISVCLDTTL
                     TTTKCNRYSFRPYDYAPAQAVAFAPYLVSKMGKKWYIAYADYAWGQSTRDAYVQEIKK
                     AGGEVFGTIGIPLGTADMTPFLSKISGDFDGLFGIFFGKDGVTIGNQAYDLGLTKKYK
                     WAGDGAIAESTNLPALGNKIEGFVGINRYVPVLEGVLNTPSHKKFMDDAVVRLKQIDP
                     SGPLPDRYVQSNYEAINALKLGMEKSGFRGREDTMKLIEALEGLEMKEGDDFPQGDKV
                     LRKEDHQAFIREFVFDIKDGKFRILEVVPKEKTIFPPDCKFVAT"
     gene            1699..2514
                     /locus_tag="VN968_22330"
     CDS             1699..2514
                     /locus_tag="VN968_22330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HXL32007.1"
                     /translation="MSVLATSRKPSATPQTGRDARLAARAGTFKGQTAGVAPGYVQGN
                     LAILPANLAGDFLRFCQLNPKPCPLLAVSAPGDFRLPTLADDLDIRTDLPRYRVFRNG
                     KLVDEPTDIRAQWRGDLVAFVLGCSFSFEEALVEDGIELRHITCNATVPMYRTSIATA
                     PAGPFHGPMVVSMRPMKPADAIRAIQITTRFPAVHGAPVHIGKPELIGIADLMKPDYG
                     DPVPVNADEIPVFWACGVTPQSVVATVKPEFCITHYPGCMLVTDRRNSEFAIM"
     gene            complement(2550..2960)
                     /locus_tag="VN968_22335"
     CDS             complement(2550..2960)
                     /locus_tag="VN968_22335"
                     /inference="COORDINATES: protein motif:HMM:NF019370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HXL32008.1"
                     /translation="MATDWTRSRDKRLLAQQAAGRTAAQIAKTLGVTRNAVIGRSRRL
                     RGIVYQSDIDSWRRANARRAQEARKRAQVRRVAQRKALRDLARAVTRGVPVGKAMSRA
                     HQAGALWRQIGAYFGISQQAAYERAKTWTQRSRS"
     gene            complement(3185..3982)
                     /locus_tag="VN968_22340"
     CDS             complement(3185..3982)
                     /locus_tag="VN968_22340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HXL32009.1"
                     /translation="MSDDIHEIYAIRYGHHDRKSSENFIGSDPHDVLQPLDFYVWAIV
                     GRSGPIILDTGFDAAMGKKRQREMIKPVDEGLKAIGIDPAEVRTIIVSHMHYDHVGNY
                     DLFPRAHYHLQDCEMAYATGRCMCHTALRLPFEVDDVVAMVRKVFAGRVTFHDGEDEI
                     VPGVTVHHIGGHSKGLQGVRVNTRRGQVVLAADAAHLYAHMESGRIFPITYNVAEVVE
                     GYETLKKLATSPDHVVPGHDPLVLARYPAAKAGLEGWVVRLDMEPKS"
     gene            complement(4070..4981)
                     /locus_tag="VN968_22345"
     CDS             complement(4070..4981)
                     /locus_tag="VN968_22345"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL32010.1"
                     /translation="MDIGFIGLGQMGFHMARRLVEAGHRLVVFDNRREAIERLTALGA
                     QAAASPRELADAVETVMVSLPTPDVVLAVATGTGGVIEGKRVRRFVDLSTTGAVMAKR
                     IFEALKTRNIVQIDCPVSGGVTGAAKGTLALMASGPRAEINAIEPALAAIGKTFFISE
                     RPGAGQTMKLCNNFLSAAAMTATSEAMVMGVKAGLDPRIMLDVINSGTGRNTATEDKF
                     GRVVLPRTFNLGFTVGLMTKDLKLCLAEGKALGVPMQVAEAVAQLLQTACDENGPDKD
                     LTTVVQPVERRAGVEVRAPRASGPLPK"
     gene            5195..5755
                     /locus_tag="VN968_22350"
     CDS             5195..5755
                     /locus_tag="VN968_22350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32011.1"
                     /translation="MPSKPDPAEMPRLDVFDEEFGQDPATILRAERRGLRLLSLVGIA
                     LGAGVIGALALAWSTADGRLRLELQSAATSPRKADRAGQDEEIDRLRGQLDALKNDVN
                     ELTEAQHQAAHTIAAMRAAEEDLRRHVPPPYWYSNPATLDLTLAKQPPPAGFAPLPRR
                     PATARSEPRDPRKREGAAPASPAAPQ"
     gene            5825..6487
                     /locus_tag="VN968_22355"
     CDS             5825..6487
                     /locus_tag="VN968_22355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32012.1"
                     /translation="MTPENLVRLLQMTGAALAVPAAAAGTFAAYQSYFSSDVTCQKLR
                     NNIVAIMERHVAPETKRSLLKKDVTEFDKLCGEGDPDARTIFQAAITEHPGDAAHAPS
                     AAPAHVDSSPQRQQTASLGVFGASGSKEHYGWVALSRRQAGSWVPHFNGYSISDTSLP
                     PPGTILSAKMMLPVWSEPQAGTNDPTKLQSRLPTGACVRVLATRGGGGRLWAEVAPAS
                     CS"
BASE COUNT         1098 a         2122 c         2086 g         1215 t
ORIGIN      
        1 tcggtgcgca gaacaggggc catcgcgcct tcccggacgg cgtcggccgg gaaggccaca
       61 gcaatggact gatcggccgt cacttaggtt gcgacgaact tgcagtccgg cgggaagatc
      121 gtcttttcct tcgggaccac ctcaaggata cggaacttgc catccttgat gtcgaaaaca
      181 aactcgcgga tgaacgcctg gtggtcctcc ttacgcagca ccttgtcgcc ctgcgggaag
      241 tcgtcacctt ccttcatctc gagcccttcg agcgcctcga tgagcttcat cgtgtcctcg
      301 cgaccgcgga aaccggactt ttccataccg agcttgaggg cgttgatggc ctcatagttc
      361 gactgcacgt agcggtcagg gagcgggccg gacggatcga tctgtttgag ccggacgaca
      421 gcatcatcca tgaacttctt gtgtgagggc gtgttgagca cgccttccag aacgggcaca
      481 taacggttta tgccgacgaa cccttcgatc ttgttgccga gcgccggaag attggtggat
      541 tcggcgatgg cgccgtcgcc ggcccacttg tatttcttgg tgaggccgag atcgtaagcc
      601 tggttgccga tcgtcacgcc gtccttgccg aagaagatgc cgaacaggcc gtcaaagtcg
      661 ccgctgatct tggacaggaa cggcgtcatg tcggcggtgc cgagcgggat acctatggtg
      721 ccgaacacct cgccgccggc cttcttgatc tcctggacat aggcgtcgcg cgtcgactgt
      781 ccccaggcgt agtccgcgta ggcgatgtac cacttcttgc ccatcttgct gacgagatag
      841 ggcgcaaacg cgaccgcctg cgccggcgcg tagtcgtagg gccggaagct gtagcggttg
      901 cacttggtcg tggtcagcgt ggtatcgagg cacacactga tgatgttgac gaccttgtac
      961 tcctcgaaca cgggcatgca ggcgaggcaa atgttggaga ggaagcctcc gacatgcacg
     1021 tcgaccttgt cctcgaccaa cagcttctcg accttacggc gcccgacgtc gggcttggac
     1081 tcgtcgtccg ctacgatcag ctcgatcggc cggccgttga tgccgccaga tttgttgatg
     1141 cggtcgaccg ccatctgcac gcccaccagc gcggtcttgc cgccggcggc gcccgcgccc
     1201 gacagcggct gctcggtacc gatcttgtac ggcttggcct gcccgaaggc ggcctgccac
     1261 ggcgctggca ccagcatgct tgcgccgagc gcacccgcgg ctgccgagcc gtatccgacc
     1321 aaccgtcggc gcgtgacctt gccgcggtac cagaacgtct caccccaatt ttgcgttttc
     1381 gcttcgtcag tcattgttgg ttcctcccag gtcggctgca atggcgcaga cacgcgccat
     1441 gacaactgcc actgtctgag cgcgcggcgc accgcgcatg gtcctgcttc cctcagtcaa
     1501 gagatgtctt gacgtttgta ggatacttca ggacacccgc gcccacaacg agcggcaggg
     1561 tgtcgcgggc atgcttatcg ctcgaggtta agccgatccc gaccctgcaa gtagggagcc
     1621 ggttttccgc ccgccgcctg atgataagat aggcaaccgg cgccggcatt ttcggctagc
     1681 gcccgacagg agaacgccat gtcggttctc gccacgtctc gcaaaccgtc cgccaccccg
     1741 cagaccggcc gtgacgcgcg gcttgcggcc cgcgccggca ctttcaaggg tcagacggcc
     1801 ggcgtcgccc ccggctacgt gcagggcaat ctcgccattt tgccggccaa cctcgcgggt
     1861 gatttcctgc ggttctgcca gctcaatccc aaaccctgcc cgttgctggc ggtttcggcg
     1921 cccggcgact ttcggttgcc gacgctcgcc gacgacctcg acatccgcac cgacctgccg
     1981 cgttaccggg ttttccggaa tggcaagctt gtcgatgagc ctaccgacat ccgcgcccaa
     2041 tggcgcggcg atctcgtcgc cttcgtgctg ggctgctcgt tctcgttcga ggaggcgctg
     2101 gtggaggacg gcatcgaact gcgccacatc acctgcaacg ccacggtgcc gatgtatcgc
     2161 acctccattg cgaccgcgcc cgccggccca ttccatgggc cgatggtcgt atcgatgcgg
     2221 ccgatgaagc ccgcggatgc gatccgcgcc atccagatca ccacgcgctt cccggcggtg
     2281 catggcgcgc cggtgcatat cggcaagccg gagttgatcg gcatcgccga tctgatgaag
     2341 cccgattacg gcgacccggt gccggtcaac gccgatgaaa ttccggtgtt ctgggcctgc
     2401 ggcgtcacgc cgcaatcagt ggtggcgacg gtgaagcccg aattttgcat cacccattat
     2461 cccggctgca tgctggtcac cgaccgccgc aacagcgaat tcgcgatcat gtgatgggtg
     2521 gtcgcgttgg acccggctgc gagccgcact caggagcgcg accgctgcgt ccaggtcttg
     2581 gccctttcgt aggcggcctg ttgggaaatc ccgaaataag ctccgatttg ccgccacagg
     2641 gcgccggctt gatgcgccct cgacatggcc ttgcctacgg gcacgccgcg cgtgacggcg
     2701 cgcgccaggt cgcggagcgc cttgcgctgc gcgacacgcc gcacttgcgc gcgttttctg
     2761 gcttcctgcg ccctgcgcgc gttcgctcgc ctccagctgt cgatatcgga ctgatagacg
     2821 atcccgcgca aacgtcttga ccggccgatc accgcgttgc gcgtcacgcc gagcgttttt
     2881 gcaatctggg cggcggtgcg cccggccgcc tgctgcgcca acagccgttt gtcgcgagat
     2941 cgcgtccagt ccgtcgccat ggctgcttcc ctcattgctc tgcgccacga gctctacccg
     3001 cgcggcagga catagtcgga atgttgcaca cgggcgttga ctcttgcaac acgaatcagc
     3061 gaaggccagg gaaattgcgg attttgattt cgttcgttcc cagcaccgcc agctcggcgt
     3121 aggaatggca cggtggtccg tggcacctcg caatcgcggc tgcggcaggc aggacgcggt
     3181 cgatttagct cttcggctcc atatcgaggc gcaccaccca gccttcgagg ccggctttgg
     3241 ccgccggata gcgcgcgagc acgagtggat cgtggccggg caccacgtgg tcgggcgagg
     3301 tcgcgagctt tttgagcgtc tcgtagcctt cgaccacttc ggctacgtta taggtgatcg
     3361 gaaatatgcg gccggactcc atgtgcgcgt agaggtgcgc ggcatccgcc gccagcacga
     3421 cctggccgcg ccgagtgttc acgcgcacgc cctgcaaacc cttggaatgg ccgccgatat
     3481 ggtggacggt gactcccggc acgatctcgt cttcgccgtc atgaaaggta acgcgaccgg
     3541 caaagacctt gcgcaccatc gcgaccacgt cgtcgacctc gaacggcagg cgcagcgccg
     3601 tgtggcacat gcagcgcccg gtcgcatagg ccatctcgca atcctgcaga tgataatgcg
     3661 cacggggaaa cagatcgtag ttgcccacgt gatcgtaatg catgtggctg acgataatgg
     3721 ttcttacctc cgcgggatcg atgccgatcg ctttcaatcc ttcgtcgacc ggcttgatca
     3781 tttcgcgctg acgcttcttg cccatggccg cgtcgaagcc ggtatcgagg atgatggggc
     3841 cggagcgtcc gacaatggcc caaacgtaga agtcgagcgg ctgcagcacg tcgtgaggat
     3901 cgctgccgat gaagttttcc gatgacttgc gatcatgatg tccgtagcgg atggcataga
     3961 tttcgtggat gtcatcgctc atggtcgtct ccgtcaggga tgcagcatca tgatgcgtag
     4021 ggagcgcagt gcgcgcggtg cgccgcgctg cgtcggggga cacggggcgt cacttcggca
     4081 gtggcccgct ggcgcgcggc gcgcgcacct ccacgccggc acggcgctcg accggctgca
     4141 ccacggtggt gagatccttg tccggtccgt tttcgtcgca ggcggtctgc aagagctgcg
     4201 cgacggcttc agcaacctgc atcggcacgc cgagcgcttt gccctcggcg agacatagct
     4261 tgaggtcctt ggtcatcaat cccacggtga agccgaggtt gaaggtgcgg ggcagcacca
     4321 cgcggccgaa cttgtcctcg gtcgcagtgt tgcggccggt gccggaattg atcacgtcga
     4381 gcatgatgcg gggatcaagc cccgccttga cgcccatgac catcgcctcc gacgtcgcgg
     4441 tcatggcggc ggcggaaagg aaattgttgc agagcttcat ggtctgcccg gcgcccggcc
     4501 gctcgctgat gaagaaggtc ttgccgattg ctgccagcgc cggctcgatg gcgttgatct
     4561 ccgcgcgcgg accggaggcc atcaacgcca gcgtgccttt cgccgccccg gtgacgccgc
     4621 cgctcacggg gcagtcgatc tgcacgatat ttctcgtctt gagcgcctcg aagatgcgct
     4681 tggccatgac cgcgccggtg gtcgacagat cgacgaagcg gcgcacgcgc ttgccttcga
     4741 tcacgccgcc ggtgccggtc gcgaccgcga gcacgacgtc gggtgtgggc aggctcacca
     4801 tcacggtttc gactgcatcg gctagctcgc gaggggaggc ggcggcttgc gccccaagcg
     4861 cggtcagacg ctcaatggct tcgcgccggt tgtcgaacac gacgaggcgg tggcccgcct
     4921 cgaccagccg gcgagccatg tggaagccca tttgtccgag cccaatgaag ccgatatcca
     4981 tcgtcgtccc tccctcgatg cgtcgttttg gtctgttgcc aacctaccac gaacgcccgg
     5041 ttgctcccac gtgaggggcg agccgcggct tgagatgccg gtgctatggg cgcatcatgc
     5101 agcgcgccgc cgcgccggac gtgggaaaat tggcgtgcag aagtttgcag cgcagtgcgc
     5161 ggcaagtaaa cgctggttaa cagtggaagc gccgatgccg agtaagccgg acccggcgga
     5221 aatgccaagg ctcgatgtgt tcgacgagga gttcggacag gaccccgcca ctattctgcg
     5281 cgcagaacgg cggggattgc ggttgttatc tctcgtcgga atagcgcttg gtgcgggcgt
     5341 catcggcgcg ctcgcgttgg cttggtcgac cgcggatggg cgactgcggc tggagctgca
     5401 atcggcggcc acatcgccgc gcaaagcgga ccgcgcaggg caggacgagg aaatcgatcg
     5461 cctcaggggc cagctcgacg cgctgaagaa cgacgtcaat gagctcacgg aagcgcagca
     5521 tcaggcggcg cataccatcg cagcgatgag ggcggccgaa gaggacttgc gacgccatgt
     5581 tccgcctccc tattggtact cgaacccggc gacgttggat ttaacactcg caaagcaacc
     5641 gccaccggct ggcttcgcgc cgctgccccg acgcccggcg accgctcgct ccgaaccgcg
     5701 cgatccgcgc aaacgtgagg gcgctgcgcc agcatcgccg gcggctccgc agtagccagc
     5761 cgcgtcgccg ttgccatttt cgtgccgtgg cggcatggtc gtaagcaggc gaccggagaa
     5821 tccgatgacc ccggaaaact tggtcagact tttgcaaatg accggggcgg cgctcgcggt
     5881 tccggcggcg gccgccggca ccttcgcggc gtaccaaagc tatttttcga gcgacgtcac
     5941 gtgccagaaa ttgcgcaaca acatcgtcgc catcatggaa aggcacgtcg cgcccgagac
     6001 caagcgtagc ttgctcaaga aggacgtgac ggagttcgac aagctttgcg gcgagggcga
     6061 tcccgacgcg cgcaccattt tccaagcagc cataaccgaa catcctggcg atgccgccca
     6121 tgccccgtca gcggcgcccg cgcacgtgga ctcatccccc cagcgccagc agaccgcctc
     6181 gctcggcgtt tttggtgctt cgggctccaa ggaacactac ggctgggtgg cgctcagtcg
     6241 ccggcaggct ggttcttggg tgccgcattt caacggctac agcatctccg atacgtcatt
     6301 gccgccgccg ggcaccatct tgtcggcgaa aatgatgctg ccggtctggt cggaaccgca
     6361 ggccggaacg aacgatccga ccaagctgca gagcaggctg ccgacgggcg cctgcgtgcg
     6421 cgtgctcgca actcgcggcg ggggcgggcg gctgtgggcc gaggtcgccc cggcatcatg
     6481 ctcgtgagca agggctgtgc ccgcgtccgc atgctggatc c
//
LOCUS       DATNOI010000555         3802 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1315376,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000555 DATNOI010000000
VERSION     DATNOI010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315376"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..208
                     /locus_tag="VN968_22360"
                     /pseudo
     CDS             1..208
                     /locus_tag="VN968_22360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596267.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(307..1278)
                     /locus_tag="VN968_22365"
     CDS             complement(307..1278)
                     /locus_tag="VN968_22365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32013.1"
                     /translation="MLKKYVSKFAMDIFPSVAATIIGAYIVNHYIATKPAVNAPAAAV
                     STTEPQAEAKPAPEAAKAGSKAPETSSDVANLPEPGIKAKGISEKAMIEKTAAEKPIV
                     VERPAEKSADKPAETASIPAATRRHQPTPREKAVAKAVPAPAQPAASVAAPVVAAPAT
                     VPAPPVEAAIVPEERRDANDLARAAIERLRGANDGSPRAQEAARIPDAARTPDPPRAQ
                     DPPRVVSPSPVRPLPPPILVSTPTAQTFDSSGSLQMRQPYTARIDDPRRPTPPADIPA
                     ASSSAPPLDLHAEAEETSRRGHTTVAEDVLSAAKSVFHAVLPKKLTD"
     gene            complement(1375..1659)
                     /locus_tag="VN968_22370"
     CDS             complement(1375..1659)
                     /locus_tag="VN968_22370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL32014.1"
                     /translation="MTAAKSEVQVDTPEVRVTEWRLGPGSATGHHIHQMDYVIVPVTS
                     GEMTIVAPNGERSKAQLGLGKSYFRKAGVEHDVLNETADEIVFLEVELKP"
     gene            complement(1688..2785)
                     /locus_tag="VN968_22375"
     CDS             complement(1688..2785)
                     /locus_tag="VN968_22375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HXL32015.1"
                     /translation="MSAHGSMPRSAWIFPALAIILFAAATALGFTFTPSPAGLVFAAA
                     LLAILFGTVFAAVHHAEVIAARIGEPYGTLLLTLAVTIIEVALIATIMLGDKPVPTLA
                     RDTVFAVVMIVCNGLVGICIMAGGLRYREQDVQVSGSSVYLSVLIVLATITLILPNYT
                     LTTPGPSYSAAQLGFVSVVTIILYGVFLYTQTILHRNYFISEVTGDADDGTHASNRMF
                     ASSVLLLLVSLLGVVLLAKKFSLVVDAGTALIGAPPAFAGILVALLILLPEGVAAIAA
                     ARKNDLQKSINLALGSSLATIGLTIPAVAITAYTLDMQLVLGLNDQEVVLLVLTFVLS
                     MLTFVTGRTNILFGLVHLVVFAVFVFLVFVP"
     gene            complement(2840..3646)
                     /locus_tag="VN968_22380"
     CDS             complement(2840..3646)
                     /locus_tag="VN968_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL32016.1"
                     /translation="MTDLKWLVVVAAVGYLGGLVTAFLMQRTILFPIPQTIRTAPDAA
                     GFPEGEEHILTTADGERVIVWHVPAKPGHQVVIYFPGNGDFLAGLVGRFRGVTSDGTG
                     LVALSYRGYAGSSGAPSERGLLWDAAAAYAFAAARYSADRLVLWGFSLGSGVAVALAA
                     DQPVGKLVLEAPYTSIADIAAAAFPILPVRWLLRDQFHSDARIAHVTAPLLIMHGGRD
                     STISITFGERLFALAHEPKQFVRFPEGGHNDLDNYGAIDRARHFIGASKG"
     gene            complement(3665..>3802)
                     /locus_tag="VN968_22385"
     CDS             complement(3665..>3802)
                     /locus_tag="VN968_22385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HXL32017.1"
                     /translation="GTVMFIKRSLASGYAGIDNPLFYRDNTMMLLGDAKKMTENIVKG
                     M"
BASE COUNT          772 a         1146 c         1209 g          675 t
ORIGIN      
        1 ccgccgccga tctcgacgag gcggcgcagg ccgatccgca gaacgcagag atcttaaccg
       61 ggggcttgcc tatgaacgtc tcggcgacaa ggccaaggcc gcgggctcat atagccgcgc
      121 catcaacctt cgccccaagg acgaagccgc gcgaaacggc tttgcccgcg tcggcggcaa
      181 gcccggccag aactacgatg cgttctagag cttccgagca aaatggatac cggttcgcgc
      241 aaagaaacgc gtcaaaacaa gctggattct gtggtctgat tcaatctgaa ccgatcgagt
      301 tctgagctag tcggtgagct ttttcggcaa taccgcgtga aataccgatt tcgccgctga
      361 cagcacgtcc tcggcgaccg ttgtgtgtcc acgccgtgaa gtctcctcgg cctcggcatg
      421 cagatccagc ggcggcgcgg atgacgaagc ggccggaatg tcggccggag gtgtcggccg
      481 acgcggatcg tcgatccggg cggtgtaagg ctgccgcatc tgcaacgatc cggatgaatc
      541 gaaggtctgg gctgtcggcg tggagaccag gatcggcggc ggaagcggcc gaaccggcga
      601 cggcgacacg acacgaggcg gatcctgcgc gcgaggcggg tccggggtgc gagccgcatc
      661 cgggatgcga gcggcttcct gcgcacgcgg cgaaccatcg tttgcgccac gcaggcgctc
      721 gatcgccgca cgcgcaagat cgttggcgtc gcgacgctct tccggaacga tggcggcctc
      781 aaccggcggc gcaggcaccg tcgctggcgc ggcaaccaca ggtgcggcaa ccgacgcagc
      841 aggctgcgcc ggcgccggaa cagccttggc gaccgccttt tcgcgcggcg tgggctggtg
      901 gcggcgcgtt gccgcaggaa tgctcgcggt ctccgccggc ttatcggcgg atttctccgc
      961 gggcctttcg accacgatcg gcttttcggc ggccgttttc tcgatcatgg ccttttcaga
     1021 gatgcccttg gccttgatac ccggctcagg gaggttggcg acatcgctgg aggtctcggg
     1081 tgccttgctg ccggcctttg cagcctcggg cgcgggcttg gcttcggcct gcggttcggt
     1141 ggtcgaaacc gcggcggcgg gcgcgttcac tgcgggtttg gtggcgatgt aatgattgac
     1201 gatataggcc ccgatgatgg tcgcggccac cgacgggaat atatccatcg caaacttcga
     1261 aacgtatttt ttcagcatgc cggccactcc gcgcggcggt tttgatgcgg gaactttgag
     1321 gcgcattaag ggatcaactg cgacggatcg gtggcaattc ggccagcggc gccgctatgg
     1381 tttcagttcg acttccagga atacgatttc atcggcggtc tcgttgagca cgtcgtgctc
     1441 gacgccggct ttgcggaagt aggatttgcc aaggccaagc tgagccttgg agcgctcccc
     1501 attgggcgcc acgatggtca tttcgcccga ggtcacggga acgatgacgt aatccatctg
     1561 atggatgtgg tggccggtgg cgctgcccgg gcccagccgc cattcggtaa cgcggacttc
     1621 cggcgtatcg acctggacct ctgatttggc ggcagtcatg gtcgatattc tccagaaatc
     1681 agcccggtca tggcacgaac accaggaata cgaacaccgc aaacaccacc aggtgaacca
     1741 gcccgaacag gatgttggtg cggccggtga cgaaggtgag catgctcagg acgaaggtca
     1801 acaccagcag caccacctcc tgatcgttga ggccgagaac cagctgcatg tccagcgtgt
     1861 aggccgtgat tgcgaccgcc ggaatcgtca gcccgatggt cgcaagcgat gaacccagcg
     1921 ccagattgat gctcttctgc aaatcgttct tacgcgcggc ggcgatggcg gcgacgccct
     1981 ccggcagcag gatcaacagc gccaccagaa tgccggcgaa ggctggcggc gcgccgatta
     2041 gcgcggtgcc cgcatcgacc acgagtgaga attttttcgc aagcagcacg acccccagca
     2101 gcgaaaccaa cagcagcagg acgcttgagg cgaacatccg attcgaagcg tgagtcccgt
     2161 catccgcatc gccggtgact tcgctgatga aataattccg atgcaggatg gtctgggtat
     2221 agagaaacac gccatagaga atgatggtga cgacactgac aaagccaagc tgcgccgccg
     2281 agtagctcgg cccaggggtg gtcagcgtat aattgggcag gatcagggtg atggttgcca
     2341 gcacgatcaa gacgctgaga taaacgctcg atccgctaac ctgaacatcc tgctcgcggt
     2401 agcgcaggcc gccggccatg atgcagatgc cgaccagacc gttgcaaacg atcatcacca
     2461 ccgcgaacac ggtatcgcgc gccaaggtag gcaccggttt atcgccaagc atgatggtcg
     2521 cgatcagcgc gacctcgatg attgtgacgg ccaaggtcaa cagcagcgta ccgtagggtt
     2581 cgccgatcct cgcggcgatc acctcggcat ggtggaccgc cgcgaacacg gtgccgaaca
     2641 ggatcgccag cagcgccgcc gcaaacacca gcccggcggg cgacggcgtg aaggtgaagc
     2701 cgagcgcggt tgctgcggcg aaaagaatga tagccagtgc cggaaagatc cacgccgatc
     2761 gcggcatcga gccgtgcgca ctcatgggcc tatatcctca aagtggggga attgactatg
     2821 cgccaaacca catcggccgt cagccttttg aagcgccgat gaaatgccga gccctgtcga
     2881 tcgcaccata attgtccaga tcgttgtggc ccccttcggg aaaccgcacg aattgtttcg
     2941 gctcgtgggc cagcgcgaac aatcgctcgc caaaggttat cgaaatggtc gaatcccgcc
     3001 cgccgtgcat gatcaggagc ggagcggtga catgcgcgat gcgcgcgtca gaatgaaact
     3061 gatctctcaa cagccagcgt accggcagga tcggaaaagc cgcagcggca atgtccgcga
     3121 tcgaggtgta gggagcttcc aggacgagct ttccgaccgg ttgatcggcc gccaatgcga
     3181 ccgcaacacc gctgcccagt gaaaaacccc agaggacaag tcgatccgcg ctatatcgcg
     3241 ctgctgcaaa ggcataggcc gctgcggcat cccagagcaa tccgcgctcg cttggcgcgc
     3301 cgctggagcc ggcatagccc cggtatgaca gagccacgag cccggtcccg tccgaggtca
     3361 cgcctctgaa gcggccgacg agtccggcca gaaaatctcc gttgccggga aaataaatga
     3421 cgacttggtg ccccggtttt gccggaacgt gccagacgat gaccctttca ccgtccgcgg
     3481 tggtcagaat gtgctcttca ccttctggaa agccggcagc gtcaggggct gtccgtatcg
     3541 tttgcggtat cggaaagagg atcgtccgtt gcatgagaaa cgctgttaca agaccgccga
     3601 gatagccgac cgctgcaaca acgacgagcc atttcaggtc ggtcatggtt tctcgtcggt
     3661 gacgtcacat ccccttgacg atgttctcgg tcatcttctt ggcgtcaccg agcagcatca
     3721 tggtgttatc gcggtagaac agcggattgt cgatgccggc atagccggat gcgagcgagc
     3781 gcttgatgaa catcaccgtg cc
//
LOCUS       DATNOI010000556         9912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2211207,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000556 DATNOI010000000
VERSION     DATNOI010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2211207"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..251)
                     /gene="rpsR"
                     /locus_tag="VN968_22390"
     CDS             complement(12..251)
                     /gene="rpsR"
                     /locus_tag="VN968_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HXL32018.1"
                     /translation="MAEAGARRPFFRRRKTCPFTGPNAPKIDYKDSKLLMRYVSERGK
                     IVPSRITAVSAKKQRELARAIKRSRFLGLLPYVIR"
     gene            complement(257..736)
                     /gene="rpsF"
                     /locus_tag="VN968_22395"
     CDS             complement(257..736)
                     /gene="rpsF"
                     /locus_tag="VN968_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HXL32019.1"
                     /translation="MPLYEHVFLARQDASTQQVEELTTQMTGIVEQLGGKVVKMESWG
                     VRSLTYRMNKNRKAHFVLLNIDGPSAAVAEIERQERISEDVIRYLTVRVEEHEEGPSA
                     MMRKADRDRDRDDRGGGFRGDRDGGFRGDREGGGGGGYRGDRGPRRPRDDADAVVEE"
     gene            1107..2066
                     /gene="fabD"
                     /locus_tag="VN968_22400"
     CDS             1107..2066
                     /gene="fabD"
                     /locus_tag="VN968_22400"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HXL32020.1"
                     /translation="MTAAFIFPGQGSQAVGMGKVLAEAFPAARAVFDEVDAALGEKLT
                     AVIWDGPAETLQLTENAQPALMAVSIATLRVLESEAGFSVPAHAAFVAGHSLGEYSAL
                     AAAGSLSVSDAARLLRTRGLAMQKAVPVGVGAMAALLGLDFEAAVAVANEAAQGQVCE
                     AANDNGAGQVVVSGDRAAVERALEIAKTRGAKRAMLLPVSAPFHCRLMQPAADVMAKA
                     LAEVKINKPASPLVSNVLAAPISDPDEIRRRLVEQVTGTVRWRESVAYMASHRVTLFF
                     EIGAGKVLSGLVKRIADGAVGISVGGPNDIVPAKDALAAAQAV"
     gene            2085..2822
                     /gene="fabG"
                     /locus_tag="VN968_22405"
     CDS             2085..2822
                     /gene="fabG"
                     /locus_tag="VN968_22405"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HXL32021.1"
                     /translation="MFDLTGRTALVTGATGGIGGAIAQALHGQGATVAISGTRREALD
                     GLAGKLGERVHVLPCNLSDAAEVEALVPSAEKAMGQVDILVANAGITRDNLFVQLRDE
                     DWDEVIQVNLTATFRLARAATRLMMRKRFGRIIAITSIVGVTGNPGQGNYVASKAGLI
                     GMIKTLGAEYAKRNITANCIAPGFIATPMTDALNDKQRETILSKVPAARLGTPEDIAA
                     AAVYLSSNEAAYVTGQTIHVNGGMAMI"
     gene            3119..3358
                     /locus_tag="VN968_22410"
     CDS             3119..3358
                     /locus_tag="VN968_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXL32022.1"
                     /translation="MSEIGERVKKIVVEHLGVEPEKVVDNASFIDDLGADSLDTVELV
                     MAFEEEFGCEIPDDAAETILTVGDATKFLEKNAKS"
     gene            3456..4721
                     /gene="fabF"
                     /locus_tag="VN968_22415"
     CDS             3456..4721
                     /gene="fabF"
                     /locus_tag="VN968_22415"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HXL32023.1"
                     /translation="MRRVVVTGLGMVTPLGCGVEPTWKRILNSESGARKIDTFDVSDL
                     TCRIACVIPRGDGTDGTFNPDQWMEPKDQRKVDDFIIFAMCAARQALDDANWHPATEE
                     DRCATGTMIGSGIGGLSGIADTALLLKERGPRKISPFFIPGRLINLASGYVSIEHGLK
                     GPNHAVVTACSTGAHAIGDASRLIALGDADVMVAGGTESPICRLSMAGFCASRALSTG
                     FNDTPEKASRPYDRDRDGFVMGEGAGIVVLEEYEHARQRGAKIYAEVIGYGLSGDAYH
                     ITSPTPDGDGAFRSMTAALKRAAITCSDIDYINAHGTSTQVGDEIELGAVERLLGNAA
                     SRVSMSSTKSSTGHLLGAAGAIECIFSVLAIRDNIAPPTINLDHPSVETAIDLVPRTP
                     RKREINVALSNSFGFGGTNASVIVQRVAN"
     gene            4861..6117
                     /gene="mltG"
                     /locus_tag="VN968_22420"
     CDS             4861..6117
                     /gene="mltG"
                     /locus_tag="VN968_22420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXL32024.1"
                     /translation="MSERPPISPRSPRAALEPERVPPPPKRSARARNPFVVFGNAVIT
                     IVLVLMIGAGGIYVYGKQKLEAPGPLQEDKVVNIPARAGMTDIADILQREGVIDNNRW
                     AFIGSVFALKARSELKPGEYSFQKSASLRDVIGTIVEGKVVQHAVTIPEGLTSEQIVA
                     RLSENDIFAGAVREMPREGTLLPETYKFPRGTSRDQVIARMQQTQKRVLAEIWERRNP
                     DIPVKTPEQLVTLASIVEKETGKADERSRVAAVFVNRLRQRIKLQSDPTIIYGLVGGR
                     GTLGRPIKRSEIQQPSPYNTYVVEGLPPGPIANPGRASLEAAANPARTRDLFFVADGT
                     GGHAFTETYDQHQKNVAKLRTMEKQIQNDTVEPADDAQPPPAGTPVDAAAPTAKPAAP
                     KKPARNAAPARQGAAQSTVAPPVVQQ"
     gene            6323..7210
                     /locus_tag="VN968_22425"
     CDS             6323..7210
                     /locus_tag="VN968_22425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HXL32025.1"
                     /translation="MALSSMTGFARSHGASGPYAFEWELKSVNAKGFDLRMRLPPGWD
                     ELESFAKKRAGEMLSRGTVYANLNVKRANAVSTIRINEEVLASIVKVAGVLAGKIDAV
                     APSIDGLLGIKGVIEVVEPETDEAEDRAAQAAAAAAFELALANLVEMRRREGVTLGQI
                     LSQRMDEIEILAKQAEAAPGRKPDAIRARLAEQIAALLESSERFDPDRLHQEAIMIAA
                     KADIREELDRIASHISQAREMIGKGGPVGRRLDFLAQEFNREVNTCCSKSNDLELTNT
                     GLAMKNVVEQFREQVQNLE"
     gene            7213..7872
                     /gene="gmk"
                     /locus_tag="VN968_22430"
     CDS             7213..7872
                     /gene="gmk"
                     /locus_tag="VN968_22430"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HXL32026.1"
                     /translation="MTAGGHGFDGLERRGLMFVLSSPSGAGKTTLSRLLIERMPGLRM
                     SVSATTRPMRPGEVEGHDYLFVDQSKFEAMVKRNELLEWATVFENRYGTPRAPVEAAL
                     SAGQDVLFDIDWQGTQQLREKARADVVSVFILPPSAADLERRLHSRAQDSDQVIRARM
                     SRASHEMSHWAEYDYIVINHDIDAAFAEVQSILKAERLKRERRTGLTTFVRALQRQLE
                     K"
     gene            complement(7974..8963)
                     /locus_tag="VN968_22435"
     CDS             complement(7974..8963)
                     /locus_tag="VN968_22435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32027.1"
                     /translation="MPNRTAKFASAIFAGLLAGTPLTTISHSAAAEEECLSGPSDKAP
                     GGGHWYYRIDRATKRHCWYQRDEGEKRSQLTPPNSAASARPVAPKPAPAAQRSVADAH
                     AELPWQSRIEQPRRDRLVSAITADAAVGEINPDVKPPVAEGQQSNMVSRWLGRSDADP
                     LNNPIFLTSPTPLAGPLPNGSVPVSPVANKDDPDTNASAVPSTQPPPAAGQFAAADLS
                     SPTHSVQMLLAAILGALALAGVMGRAIFKFVGPRSPAKRKGRNRRSAIWKSTDGGRTT
                     SNAHPGADALSPRPDFPRDLDQVDDPGDRIAELLRDFSRRGRKGGKTRQPSPT"
     gene            complement(9132..>9912)
                     /locus_tag="VN968_22440"
     CDS             complement(9132..>9912)
                     /locus_tag="VN968_22440"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32028.1"
                     /translation="EHYPWTMSWTPHYASEGEIYARYILSAKPDAKIGILSQNDDLGR
                     DYLLGFKRALGDKASTMIVSSLTYNTSDPTIDSQVVSLKASGADVFFIVTVPKFSAQA
                     IRKAYDIDWHPQEFVVSVGSSIAGAIRPAGFEAAKGIISAAYQKDPADPQWRDDPAMQ
                     AWNVWMDKYNPRVDKSDYYAPYGYNIGFAVVRLLKQCGDNLTRENIMQQASHLDMELP
                     LLLPGIRLKTSPTDLRPIKQMRLVRFDGQRYVLFSDVLASE"
BASE COUNT         1912 a         2953 c         3232 g         1815 t
ORIGIN      
        1 gaagagtttt tttagcgaat gacgtaaggc agcagaccga ggaaacgcga gcgcttgatg
       61 gcgcgcgcga gttcgcgctg cttcttggcg gagacggcgg tgatgcggct cggcacgatc
      121 ttgccgcgct cggacacata acgcatcaac agcttggaat ccttgtagtc gatcttcggc
      181 gcattcggac ccgtgaacgg gcaggtcttg cgacggcgga aaaacggacg gcgtgcacca
      241 gcttcggcca tgattcttac tcctcaacca ctgcgtcggc gtcatcgcgc gggcgacgtg
      301 gaccacggtc gccgcgataa ccgccaccgc cgccaccctc acgatcgccg cggaatccgc
      361 catcgcggtc gccacggaaa ccgccgccac ggtcgtcgcg gtcacggtcg cgatcggcct
      421 tgcgcatcat cgccgaggga ccttcctcgt gctcctcgac gcgaacggtg agatagcgga
      481 tcacgtcttc gctgatccgc tcctggcgct cgatctcggc gaccgccgcc gaggggccat
      541 cgatgttgag cagcacgaaa tgcgccttgc gattcttgtt catccgatag gtcagggagc
      601 gaacgcccca gctttccatc ttcacgacct tgccgccgag ctgctcgacg atcccggtca
      661 tctgggtggt caattcctcg acctgctggg tgctcgcatc ctgacgcgcg agaaaaacat
      721 gctcatagag aggcatgggt gtcctttcct gtgttggcgc ggttcccggc ggcaagccct
      781 tcgaaccctt cggaaaggac tcgatatgct cagaaggcgg aagcacggga cgacggaccg
      841 actggccctg ccacatcaac atcgccggag aggtgaaatt gctgagaccg tccgttcagc
      901 tcccggccgg gatccacgga tggcgcggtt tataagggtt ttgggggcga tggcaaggga
      961 aaggcaaggg aaagacggag ggaaaggcga gccggccccc agcatctccc ggtggcccgg
     1021 aaccggtggc ggcttgacac aataggcccc tgcagtgtct ttcggcacaa ctttagggat
     1081 ttcagggacg ttcaggggcg gcgccgatga cggcagcatt catttttcca ggacagggct
     1141 cccaggcggt tgggatgggc aaggtgctgg cggaggcgtt tccggcggcc cgggcggtat
     1201 tcgacgaggt cgatgccgcg ctcggcgaaa agctcacggc ggtcatctgg gacggtccgg
     1261 ccgaaaccct gcagctcacc gaaaacgccc agccggcatt gatggcggtc tcgatcgcaa
     1321 ccttgcgggt gctggagtcc gaagccggct tctcggtccc ggcgcatgcc gcattcgtgg
     1381 cgggacattc gctcggcgaa tattccgcgc tcgccgccgc cggcagcctc agtgtcagtg
     1441 acgccgcgcg cctgctgcgc acccgtggcc ttgccatgca gaaggcggtc ccggtggggg
     1501 tcggcgcgat ggccgcattg ctgggtctcg atttcgaagc cgcggtcgcc gtcgccaacg
     1561 aggccgcgca gggtcaggtc tgcgaggcgg ccaatgacaa tggcgccggt caggtcgtgg
     1621 tctccggcga ccgggccgcg gtcgagcgcg cgctcgaaat cgccaagacc aggggcgcca
     1681 agcgcgcgat gttgctgccg gtgtcggcgc cgtttcattg ccggctgatg cagcccgcgg
     1741 ccgacgtgat ggccaaggcg ctggcggagg tgaagatcaa caagccggcg tcgccgctgg
     1801 tgtccaacgt gttggccgcg ccgatttccg atccggacga gatccgccgc cgcctggtcg
     1861 aacaggtcac gggaaccgtg cgctggcgtg agtcggtggc ttacatggca agccaccgtg
     1921 tcacactttt cttcgaaatc ggcgccggca aggtgctcag cgggttggtc aagcgcattg
     1981 ccgatggcgc ggtcgggatt tcagtcggcg gcccaaatga tattgtaccg gcgaaagacg
     2041 cgctcgccgc cgcacaggcg gtttgagcga aggagagacg aagaatgttc gatctgactg
     2101 gcaggacggc gctcgtcacc ggcgcgaccg gtggcatcgg tggcgcgatc gcacaagcgc
     2161 tgcacgggca aggcgcgacg gtggccattt ccgggacgcg gcgcgaggcg ctggatgggc
     2221 tcgccggcaa attgggcgag agggttcatg tgctgccgtg caatctctcc gacgccgccg
     2281 aggtcgaggc gctggtaccg tctgccgaaa aggccatggg ccaggtcgac attctcgtcg
     2341 ccaacgccgg catcacccgg gacaatctgt tcgtgcaatt gcgcgacgag gactgggacg
     2401 aggtcatcca ggtcaatttg accgcgacct tccgtttggc gcgggcggcg accaggctga
     2461 tgatgcgcaa gcgcttcggc cgcatcattg cgatcacgtc gatcgttggc gtgaccggca
     2521 atccgggtca gggcaactac gtggcgtcga aggccggact gatcggcatg atcaagacgc
     2581 tgggggccga atacgccaaa cgcaacatca ccgccaattg catcgccccg gggtttattg
     2641 cgacgccgat gaccgacgca cttaacgaca aacagcgcga aacgatcctt tccaaggttc
     2701 ctgcggcgcg actcggaacg cccgaagata tcgctgcggc ggcggtctat cttagttcga
     2761 atgaagctgc gtatgtcacc gggcagacca ttcacgtcaa cggtgggatg gccatgatct
     2821 gagccggttg ccaatggccc tgggcggggc gaaaggccgt ttccgaagcc cgattgaggc
     2881 ttatagtcaa ggcttgggaa gtatgataac cgaaccctcg acggaaaggc aaagaacgcc
     2941 attgcaggat tttgaaaccc tgtatattgg cggtggggag cctgcccgcg ttcgccgggc
     3001 ctttgtcggc tacgacgggg ccaaatcaag actacgcgcg attgcgaagg aagtttaacg
     3061 accacgatgg ctcgtacgtc ttgcggtcga gtccaatgga acaagcacga ggttgaagat
     3121 gagtgagatt ggcgagcggg ttaagaagat tgtggtcgaa caccttggtg ttgaacccga
     3181 aaaagtggtc gacaacgcga gcttcattga cgacctcggc gccgacagcc tcgataccgt
     3241 cgagctcgtg atggcgtttg aagaagaatt tggttgcgaa attcccgacg atgcggcgga
     3301 gacgattttg accgtcggcg acgctacaaa atttctagaa aagaacgcga aaagctgacc
     3361 cccctgcgtg gtgccgagga agccggacgg gccgtggtcg tacggtccac cgatttcttg
     3421 ttgtgggcgg caaatcccgg gctggagtgc tggatatgag gcgggttgtc gtgacgggcc
     3481 tcggcatggt gacgcctctc ggctgcggcg tcgagccgac ctggaagcgc atcctcaaca
     3541 gcgagagcgg cgccagaaag atcgatacat tcgatgtctc tgatctgacc tgtcggatcg
     3601 cttgcgtgat tccgcggggc gacggcaccg acggcacatt caaccccgac cagtggatgg
     3661 agccgaagga tcagcgcaag gtcgacgatt tcatcatctt cgcgatgtgc gcggcgcgcc
     3721 aggcgctcga tgacgccaat tggcatcctg ccaccgaaga ggatcgctgt gccaccggca
     3781 cgatgatcgg ctctgggatc ggcggcctct ccggcattgc cgatacggcg ctgcttctca
     3841 aggagcgcgg cccgcgcaag atatcgccgt tcttcattcc cgggcgcctg atcaatcttg
     3901 cctcgggcta tgtgtcgatc gagcacggcc tgaagggtcc caaccatgcg gtggtgaccg
     3961 cttgctcgac cggcgcgcat gcgatcggcg atgcaagccg gctgatcgcg ctcggcgatg
     4021 ccgacgtgat ggtggccggc ggaaccgaat cgccgatttg ccggctgtcg atggcggggt
     4081 tctgtgcgtc gcgcgcgtta tcgaccggct tcaacgatac ccctgaaaaa gcatcgcggc
     4141 cctacgaccg ggatcgcgac ggtttcgtga tgggggaggg ggccggcatt gtggtgcttg
     4201 aagaatacga gcatgccagg cagcgcggcg cgaagatcta tgcggaggtg atcggttacg
     4261 ggttatccgg cgacgcctat cacatcacgt cgccgacgcc ggatggcgat ggcgcgttcc
     4321 gcagcatgac tgctgcgctg aagcgcgcgg caatcacttg ttccgatatc gactacatca
     4381 acgcgcatgg aacgtcgacg caggtaggtg acgagatcga actcggtgcg gtggagcggt
     4441 tattgggcaa cgccgcttcc agggtgtcga tgtcgtcaac caaatcgtcg accgggcatc
     4501 ttctgggggc ggcgggagcg atcgagtgta tcttcagcgt tcttgcgatt cgcgataata
     4561 tcgctcctcc caccatcaat ctcgaccacc cgtcggtaga aacggcgatc gatctcgtgc
     4621 cgcgaacgcc acgcaagcgc gagatcaatg tcgcgctttc gaactcgttc ggtttcgggg
     4681 gcaccaacgc atccgtgatc gtgcagcgcg tcgccaactg acgcgccatg tgaatcatgt
     4741 aaagaagtgt ccaccgattc gccgcattcg gcgatgaatc ctaggattgg gcgtagacga
     4801 ccggccgacg tggaaatctc gcgaggccgc gattgaatcg gcaggattta ggtcacatcg
     4861 atgagtgaga ggccgcccat ttcgccaagg agtccgcgcg cagcgctgga gcccgagcgg
     4921 gtcccgccgc cgccgaagcg atccgcgcgc gcccgcaatc cattcgtggt cttcggcaat
     4981 gccgtcatta ccatcgtgct ggtcctgatg atcggcgcgg gcgggatcta tgtctacggc
     5041 aagcaaaagc tggaagctcc ggggccgctg caggaggaca aggtcgtcaa tattccggcg
     5101 cgcgcgggaa tgaccgacat tgccgatatc ctgcagcgcg aaggcgtcat cgacaacaat
     5161 cgctgggcgt tcatcggcag cgtgttcgcg ctgaaggcgc gctccgaact caagcccggc
     5221 gaatactcgt ttcagaagag cgccagcctg cgcgacgtga tcggtaccat cgtcgaaggc
     5281 aaggtggtcc agcacgccgt cacgataccg gaaggcctga cctccgagca gatcgtggcc
     5341 cggctttccg agaacgacat tttcgcgggc gcggtccggg aaatgccgcg cgagggtacc
     5401 ttgctgccgg aaacctacaa gttcccgcgc ggcacctcgc gcgaccaggt gattgcgcgc
     5461 atgcagcaga cgcagaagcg ggtgctggcc gaaatatggg aacgccgcaa tccggacatt
     5521 ccggtcaaga cgccggagca actcgtcacg ctggcatcga tcgtcgagaa ggaaaccggc
     5581 aaggcggacg agcgcagccg ggtcgctgcg gtgttcgtca atcggctgcg gcaaagaata
     5641 aagctgcagt ccgatccgac catcatctat gggctggttg gcggcagggg aacgctgggg
     5701 cggccgatca aacgctccga aattcagcag ccgtccccct ataataccta cgtggtcgag
     5761 gggcttccgc cggggccgat cgccaatccc gggcgtgcct cgctggaagc cgccgccaat
     5821 ccggcacgca cccgcgatct gttctttgtc gcggacggca ccggcggcca cgcctttacc
     5881 gagacgtacg accagcatca gaagaacgtc gccaagctgc gcaccatgga aaaacagatt
     5941 cagaacgaca cggtcgaacc ggccgacgat gcccagccgc ccccggcagg tacgccggtc
     6001 gacgccgccg cccccacagc caagccggca gccccgaaaa agcccgcccg caatgccgct
     6061 ccggcccggc agggagcggc gcagtcgacg gtggcgcctc cggtggttca gcaatagtgc
     6121 aagcccgccg gcatgcgccg gagcctcgcc ggcatgcgcc ggagcctgat ggcacatggc
     6181 ccggaaaacg ctgctaactc gtaacttgtg cgtattctac ttgcaaaacc cgtatccact
     6241 tttgcgcaat acgcgctaag gtcacagcag tggttggctt tgcgaatcac accccctgtt
     6301 ccgttccgga gtttatttcg cgatggcgct gtcgagcatg actggttttg cccgaagtca
     6361 cggcgccagc ggaccctatg cgttcgaatg ggaactgaag tccgtcaacg ccaagggttt
     6421 tgatctgcgg atgcggctgc cgcccggatg ggacgaactc gaaagcttcg ccaagaagcg
     6481 tgccggcgaa atgctgtcgc ggggtacggt gtacgccaat ttgaacgtca agcgcgccaa
     6541 cgcggtctcc acgatccgca tcaacgagga ggtgctcgcc tcgatcgtca aggtcgccgg
     6601 cgtgctcgcc ggcaagatcg atgcggtagc gccgagcatc gacgggcttt tgggaatcaa
     6661 gggggtcatc gaggttgtcg agccggaaac cgacgaggcc gaagacaggg ctgcgcaggc
     6721 tgccgcagcg gcggcattcg aactggcgct cgccaacctc gtcgagatgc gacggcgcga
     6781 gggcgtgacg ctcgggcaga tcctctcgca gcgcatggac gagatcgaga tcctggcaaa
     6841 gcaggccgag gccgcacccg gacgcaagcc cgacgcgatc agggcacggc ttgccgagca
     6901 gatcgcggca ctgttggaat cgtccgaacg cttcgatccc gaccggctcc atcaggaggc
     6961 gatcatgatc gccgccaagg ccgatattcg cgaagaactc gaccggatcg cctcgcatat
     7021 ctcgcaggcg cgcgagatga tcggcaaggg cggaccggtc gggcggcgtc tcgatttcct
     7081 ggcgcaggaa ttcaaccgcg aggtcaatac ctgctgctcc aagtcgaacg acctcgaact
     7141 gaccaacacc ggccttgcga tgaagaatgt ggtcgagcaa ttccgcgaac aggttcaaaa
     7201 tctggagtga ccatgacggc tggcggccat ggcttcgacg ggcttgagcg gcgcggattg
     7261 atgttcgtgc tgtcgtcgcc gtcgggcgcg gggaagacca cgctgtcgcg tttgctgatc
     7321 gaacggatgc cgggactgag gatgtcggtg tccgcgacca cgcggccgat gcggcccggc
     7381 gaggtcgaag gccacgacta cctgtttgtc gaccaatcca aattcgaggc gatggtaaag
     7441 cgaaacgagt tgctcgaatg ggccaccgtg ttcgaaaatc gctatgggac gcctcgcgcg
     7501 ccggtcgaag cggccttgtc ggcgggacag gatgtgctgt tcgatatcga ttggcagggc
     7561 acgcagcaat tgcgggaaaa ggcgcgggcg gacgttgtca gcgttttcat cctgccgccg
     7621 tccgccgccg acctggaaag acgcttgcac tcccgcgcgc aggattccga tcaggtcatc
     7681 cgggcacgga tgagccgcgc cagccatgaa atgagccact gggccgaata cgattatatc
     7741 gtcatcaatc acgatatcga tgccgcgttt gccgaggtgc agtcgattct gaaggcggaa
     7801 cggctgaagc gcgaacgccg cacgggtttg acgacgttcg tgcgcgcatt gcagcggcaa
     7861 ttagagaaat agcccggcgc tcagagcgtt ttcaagcgac cggctcgcgt caagaaaacg
     7921 cgtcacatcg agaatccaga gccgcgtttc gattctatcg acgcgaaatg gctctaggtt
     7981 gggctgggtt ggcgcgtctt tccgcccttg cgacccctgc gggaaaaatc ccgcaacaat
     8041 tccgcaatcc tgtcaccggg gtcatcgacc tgatcaaggt cacgagggaa atccggtcgc
     8101 ggcgacaggg catccgctcc tggatgggcg tttgatgtcg tgcgaccgcc gtcggtcgat
     8161 ttccagatcg cgctccggcg attgcggcct ttacgctttg cagggcttcg ggggccgacg
     8221 aatttgaaaa tcgcgcgtcc catcacgccg gccagcgcca gcgcgcccaa aatagcggcc
     8281 aacagcatct gcacggagtg ggttggagac gacaggtcgg ccgcggcgaa ctgaccggca
     8341 gcgggcggtg gctgagttga cgggactgcg ctcgcgttcg tatcgggatc gtccttgttt
     8401 gcgacgggac tgactggcac agaaccattt ggcaaaggac cggccagggg ggttggactg
     8461 gtcaggaaaa tcgggttgtt caaaggatcc gcgtccgacc gacccaacca ccgcgaaacc
     8521 atgttcgatt gttgtccctc cgcaaccggt ggtttcacat ccgggttaat ttcgccgacc
     8581 gcggcatcgg cggttattgc cgaaaccagc ctatcgcggc gtggctgttc gatacgcgac
     8641 tgccaaggta gttcggcgtg cgcgtctgcg accgaacgct gcgccgctgg tgccggtttc
     8701 ggcgcaaccg gacgcgccga tgcagcggaa ttgggtgggg tgagttgaga acgcttttca
     8761 ccctcgtctc gctggtacca gcaatggcgt ttggtggcgc gatcaatacg atagtaccaa
     8821 tggccgcccc caggcgcttt gtcgctcggg ccggaaaggc actcctcctc cgccgccgcg
     8881 ctgtgtgaaa ttgtcgttag cggagtcccc gcgaggaggc cggcgaagat cgctgacgcg
     8941 aatttcgcgg ttcggtttgg catcggtatc tccggcttac gcaacactcc gtgtcccaat
     9001 tcttaacaaa aacttgggtc gtaatgagcc gcaattccgg acagcatgag gcttaattcg
     9061 ggctcgcgct tggatttgcg cgactggcag cgttcaaggg aaataaaggt tgaagctgtt
     9121 gttttggcgc atcattcgct ggcgagcacg tcgctaaaca gcacgtagcg ctggccgtcg
     9181 aagcgcacca gccgcatctg cttgatcggg cgcaggtcgg tcgggctggt cttcaggcgg
     9241 attcccggca gcagcaacgg cagctccata tccagatggg aagcctgctg catgatgttc
     9301 tcgcgcgtca gattgtcgcc gcactgctta agcagccgga ccaccgcgaa accgatattg
     9361 tagccgtaag gggcgtaata atcgctcttg tcgacgcgcg ggttgtactt gtccatccag
     9421 acgttccagg cttgcatggc cgggtcgtct ctccactgcg ggtcggccgg gtccttttga
     9481 taggccgccg agatgatgcc tttggccgct tcaaacccgg ccggcctgat cgcgcccgcg
     9541 atcgaggagc cgacgctgac cacgaattcc tgcgggtgcc agtcgatgtc ataggctttg
     9601 cgaatggcct gcgcggagaa tttgggcacc gtgacaatga aaaatacgtc cgcgcccgac
     9661 gccttcagcg aaaccacctg ggaatcgatg gtcgggtcgc tggtgttgta ggtcaatgac
     9721 gacacgatca tcgtcgacgc cttgtcaccg agcgcgcgtt tgaacccgag gagatagtcg
     9781 cggccaagat cgtcgttctg ggacaggata ccgatcttag cgtcgggttt cgccgacaga
     9841 atatatctcg cgtagatctc gccttccgat gcataatgcg gcgtccagct catggtccac
     9901 ggataatgct cg
//
LOCUS       DATNOI010000557        14835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2211250,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000557 DATNOI010000000
VERSION     DATNOI010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 14835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14835
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2211250"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="VN968_22445"
     CDS             complement(<1..1291)
                     /locus_tag="VN968_22445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007796849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HXL32029.1"
                     /translation="MVTWKPDPTFYPSPRMAMKAPAEKLAYVAAFDASRKAPDGIAVV
                     DLDPDSKHYSTILNTVAMPNVGDELHHFGWNACSSCLCPNAPHPHVERRYLVVPGLRS
                     SRIHILDTKADPTKPAIVRVIEPEEVAEKAGYTRPHTIHCGPDGIYVAALGNAEGKAP
                     GGIFLMDHESFDILGRWEIDRGPQQLAYDAWWHLGHDTLVTSEWGTPDTFENGLVPEI
                     LLGAKYGRKLHFWDLHTRKHQQTIDFGDKYQLVFELRPAHDPTKAYGFVNCVISLEDL
                     SSSIWAWHRDGEKWGVSKVIDIPAEPADPDQLPPLLKGFKAVPPLVTDIDLSMDDKFL
                     YVACWGTGDLRQYDVSDPFKPKLTGKVRIGGIVSRATHPGAKNGALSGGPQMVEISRD
                     GRRVYFTNSLYGAIDEQFYPDGIEGWMVKLDAKPEGGI"
     gene            1632..3272
                     /locus_tag="VN968_22450"
     CDS             1632..3272
                     /locus_tag="VN968_22450"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HXL32030.1"
                     /translation="MNGTARFRARDRSSETLEFDYVVVGAGSAGCVLANRLSADGRHS
                     VLLLEAGPKDNNLWIHVPLGYGRLFKEKTVNWMYQTEPEPGLDGRTIFQPRGKVLGGS
                     SSINGLLYVRGQHQDYDRWRQHGNSGWGFDDVLPYFKKAENQERGADDFHGAGGPLPV
                     SDLVHADRLSAAFIAAAAETGIPVNPDFNGASQEGAGFFQTTTRRGRRASTAVAYLRP
                     ARGRRNLQVETSALAQRILFDGHRADTIEFRRAGVLRTARARKEILVSSGAYNSPQLL
                     QLSGVGPADLLRQHGIDVVLDAPGVGHDLQDHMQVRVVMRCTQSITLNDVVNSPVRRI
                     FAGARYAAFRKGPLTIAAGTSGAFFKTSPRLATPDIQIHFLPFSTDKMGERLHSFSGF
                     SASVCQLRPESRGSLRIRSADPTAAPEIRINYLSTEVDRTANVEGLKILRSILRAPAL
                     RPYVMEEVDPGARVSTDEELLNYCRARGSTIYHPTSTCRMGSDPLAVVDQRLRVRGLE
                     GLRVVDGSIMPDLVSGNTNAAIIMIAEKASDMILEDAR"
     gene            3375..4355
                     /gene="hemC"
                     /locus_tag="VN968_22455"
     CDS             3375..4355
                     /gene="hemC"
                     /locus_tag="VN968_22455"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HXL32031.1"
                     /translation="MAIRIRIGTRKSVMALAQTEEIARRLTAADPDLDVEIVKFETSG
                     DSDQTGRLLQHGGKGGAFVAEIRAAVVSGELDAAMHSLKDMPGNEDTPGLVIGATLER
                     DPPGDALVLRSGVSIDDLRRARGKGFKIGTNAVRRAAYARRLFPDIEVIHYRGAADTR
                     VRKLDNLEMQRLPDGGAVGPADALIMARSGLERVGLAGRIAYEFSIREMLPAVGQGIV
                     AVECAIDDWQTRRMLARIDDPAAHQSADAEREVLWVLNGHCNSPIAGFSTIDGAQMSL
                     TASVLDEAGGLFIEATRSGPANRPRELGRAVGFDLLAKGAAEIIERSRPG"
     gene            complement(4411..5448)
                     /gene="hemE"
                     /locus_tag="VN968_22460"
     CDS             complement(4411..5448)
                     /gene="hemE"
                     /locus_tag="VN968_22460"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HXL32032.1"
                     /translation="MHALKPFLEVLSGRRQPVPPIWMMRQAGRYLPEYRELRCRAGGF
                     LDLCFTPEYAAEVTLQPIRRFNFDAAIIFSDILVIPYALGREVRFEVSEGPRLDPLET
                     PEQVGTLARHGDFSKLEPVYEALRRVRRELDPNIALIGFCGAPWTVATYMVAGQGTPD
                     QAPARMMAYRHPEAFSTIIDVLVENSIQYLLGQLKAGADVLQIFDTWAGVLPPREFAR
                     WSTEPTRRIVEGVRAKMPDAKIIGFPRGAGALLPGYVDATGVDAVSIDWAAEPSLIRE
                     RVQSRVAVQGNLDPLVLIAGGAALDRAIDDVLENYAGGRLIFNLGHGIQPETPIAHVE
                     QMIKRVRTHQG"
     gene            5962..6183
                     /locus_tag="VN968_22465"
     CDS             5962..6183
                     /locus_tag="VN968_22465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32033.1"
                     /translation="MAEKFDPAAHDRHAANPREAAAADREIHAKLEAGLIDSFPASDP
                     VSAAQPAPSKADGDREGTSLWAKVRAVFR"
     gene            6237..6371
                     /locus_tag="VN968_22470"
     CDS             6237..6371
                     /locus_tag="VN968_22470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32034.1"
                     /translation="MKHEFDHAANDSPRSGSSKQVTPLLWGAIVVAIVVLILWFAIKA
                     "
     gene            6468..6737
                     /locus_tag="VN968_22475"
     CDS             6468..6737
                     /locus_tag="VN968_22475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32035.1"
                     /translation="MKRFLAMAVAAAAICVAMPVGAEEIGVGVGPDGVTVGTVRGDRD
                     RDRDRDREVIRERDHRDRDTVVIQRGRDHDRDYDRDRKVIIDRDR"
     gene            complement(6801..7016)
                     /locus_tag="VN968_22480"
     CDS             complement(6801..7016)
                     /locus_tag="VN968_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HXL32036.1"
                     /translation="MSDIKALSERIDVLETRLAFQDETIETLNQTITAQWQQIDALTR
                     QIANLSERLQEAETHSPGAANEPPPHY"
     gene            complement(7009..7761)
                     /locus_tag="VN968_22485"
     CDS             complement(7009..7761)
                     /locus_tag="VN968_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HXL32037.1"
                     /translation="MPHRVAAFYQFAALPDFRELREPLRAVCAGLGLKGSVLLAHEGI
                     NGTLAGQPEAIAALVEELQCGALFDGRLDNLELKFSHAAAMPFQRLKIRLKKEIVTLG
                     DRHADPTRQVGVYVEPGDWNALIAGPDTLVIDTRNAFEVAMGTFPGAVDPGIRSFGQF
                     KDFAARHLDPAKHQRIAMFCTGGIRCEKASAYLLARGFAQVYHLKGGILGYLEGVPQA
                     ESRWRGECFVFDDRVALGHGLRQRRTEQDSDE"
     gene            7937..9523
                     /gene="ggt"
                     /locus_tag="VN968_22490"
     CDS             7937..9523
                     /gene="ggt"
                     /locus_tag="VN968_22490"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXL32038.1"
                     /translation="MRNFHLAGRSTVHAQNAMVATSHPLASLTAIEVLRSGGTAADAA
                     VAACALLGVIEPQSTGIGGDCFALLQRKGEGKIMAYNGSGRAPRAADASWYLARNMHA
                     IPLTSAHAVSIPGAIDAWVTILRDHGKLGLDSLLQPAIKAAEEGYVVAPRIAFSWKNE
                     FDKLKKGTNTERYLLPHGKAPVAGDVIHQPELGQTLRAIASGGRDAFYKGAIAEDMVE
                     TLRGIGGLHTLDDFAAHTTETTTPIGTVYKGHHVWQCPPNGPGITMLVMLNILSRFDL
                     TKFPPMSVERFHLEAEAARIAYMMREQYIGDPAFVDVDVVRILTKEFAEEYGSKIRMD
                     RLLDLPNVSPPMNPSTVYVTVVDRDRNVCSFINSIAHAFGSAIVSNKTGILLQNRGGG
                     FRVQPGHPNCIAPGKRPLHTIIPSLVTKAGRAVMPFGVMGGQYQPVGQVHVLTNVLDY
                     GCDVQEAIDMPRGLHYEGVYQLEDGVPANIVDGLRKLGHQTASLVTPHGGGQAIWIDW
                     DKGTLTGGSDPRKDGCALGY"
     gene            9643..10032
                     /locus_tag="VN968_22495"
     CDS             9643..10032
                     /locus_tag="VN968_22495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXL32039.1"
                     /translation="MPPSVSAQTAGKTMTTPSGLQITDSTVGTGATPKPGQICVMHYT
                     GWLYEGGKKGKKFDSSVDRNEPFEFPIGQRKVIAGWDEGVATMKVGGKRTLIIPPALG
                     YGARGAGGVIPPNATLMFDVELLDVKG"
     gene            10077..11054
                     /locus_tag="VN968_22500"
     CDS             10077..11054
                     /locus_tag="VN968_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007800644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HXL32040.1"
                     /translation="MQISILDDYFDTLRTLKCFGKLAGHDVTVWNDHVQDVETLAERL
                     CDTEALVLIRERTQIRNALLERLPKLKLISQRSVYPHIDIDACTRLGIIVSSSQHADT
                     PSYATAEFTWGLVLAAMRQIPQQMAALKAGNWQIGVGHTLRGKTLGIYGYGRIGAVVA
                     GYGKAFGMEVLVWARKPALAKARADGYATASSKAAFFEQCDVLSLHMRLVDATRGIVT
                     AADLARMKPTALLVNTSRAPLIEPNALVNALRAGRPGMAAVDVYEKEPLRDTSDPLLN
                     MDNVVATPHLGYVSRDEYEIQFTDIFDQITAYATGTPTNVVNPDAIGKR"
     gene            complement(11120..11323)
                     /locus_tag="VN968_22505"
     CDS             complement(11120..11323)
                     /locus_tag="VN968_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HXL32041.1"
                     /translation="MGATADKVKGATNEAIGKAKRGVGEAVGSDKMQGEGAIQEIKGK
                     GQKAMGDAKEATNKTAAAANKKL"
     gene            11459..11980
                     /locus_tag="VN968_22510"
     CDS             11459..11980
                     /locus_tag="VN968_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL32042.1"
                     /translation="MTGPHDHTHADHSHDHDDPARWKHDGVRVIPGDQLDPNVPSTAG
                     MDRKAAINFARVGAQKLWAGTVTIKPDAKTGAHHHGHLESIIYVVKGKARMRWGEHLQ
                     FMAEANPGDFIFIPPYVPHQEINASPDQVLECVLVRSDGEAVAINLDIEPVEKPETVL
                     WVDPVHRDPAATK"
     gene            complement(12033..13559)
                     /locus_tag="VN968_22515"
     CDS             complement(12033..13559)
                     /locus_tag="VN968_22515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL32043.1"
                     /translation="MSNQLTDAELAALMPSELCQYKTPIPTQIVASDEFYPDPQNERQ
                     REVESRLLAMADDLGGKQGLDRRRFFQSAAGMAASFVAMNEVYGPLFDVTRAEAATPA
                     MAEERAGALKDQFIMDMHTHFLRDDTRITTFVDMRKAVGKAGWNKQLADHEQTIEDLK
                     FNNYKKEMFLDSDTKISLISSAPSDIEQDWFLTNEQMADARKTINDEAGSRRVFCHMI
                     FTPGQPGWLDKLDAGLALKPESCKGYTVGDNTHKEISRYPWRMDDEKVAYKGYEKMVK
                     AGIKNVCIHKGLFAPGVEKQYPNLRGFADVADVGQAAKDWPQLNFVIYHSAYRHVGGD
                     PKQALAEFERTGRISWTSDLADIPAQYGVNNVYGDVGQLFATTLVSEPNVCAALMGTL
                     IKGLGVDHVCWGTDALWTGAPQWQIEGLRRLEIPEAMQKKFGYAPLGAVDGPVKSAIF
                     GGNNARLYDIQPKRAMLELKGDRFAVMKAAYEKAGPEPSNTRYGYVVPNGTMDHKVFA
                     "
     gene            13823..>14835
                     /locus_tag="VN968_22520"
     CDS             13823..>14835
                     /locus_tag="VN968_22520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL32044.1"
                     /translation="MEKFDRPLARRTVIRGAGLGLVAGGLASSLPAPSTSAAAAEGGE
                     IWSSEYWAKKGDVPLWMYRKRVGAPKAGEPARPVVFFVHGSSVTSRVFDLKVPGRGEY
                     SVMDEFARYGFDCWTMDHENYGKSGRTSGNSDIASGVEDLKVAAEIVTRETGQKRFHF
                     VGESSGALRAGAYAMAAPERIDRLVFAAFTYKGEGSPTLTKRAEQLDYYRTHNMRKRD
                     REMIRSIATRDKPGTSDPAAVEVLADVEMQFGDQIPTGTYLDMTANLPVVHPDKVLAP
                     VLLVRGEYDGIATVADLEEFYNQLPNGDRQFIILPGTAHSVALATNRQLFWHVTRAFL
                     TMPA"
BASE COUNT         2731 a         4563 c         4708 g         2833 t
ORIGIN      
        1 tgatgccgcc ctctggcttg gcgtcgagct tcaccatcca gccttcgatg ccatcgggat
       61 agaactgctc gtcaatggcg ccatagagcg agttggtgaa atagacccgg cgaccatcgc
      121 ggctgatctc caccatctgc gggccgccac ttagggcgcc gttcttggca ccggggtgcg
      181 tcgcgcgaga tacgatgccg ccgatccgga ccttgcccgt caatttcggc ttgaagggat
      241 ctgaaacgtc gtattggcgc agatcacccg tgccccagca cgcgacgtac aagaacttgt
      301 cgtccattga gaggtctatg tcggtaacga gtggtggcac cgccttgaag cctttgagga
      361 ggggcggcag ctgatcgggg tcggcgggtt cggccgggat gtcgattacc ttgctaaccc
      421 cccacttctc gccgtcacga tgccaagccc aaattgagga agagaggtct tccaggctta
      481 tgacgcagtt gacgaaccca tacgcctttg tcgggtcatg agccggacga agctcgaaca
      541 cgagctggta tttgtcaccg aagtcgatgg tctgctgatg cttgcgggtg tgtaggtccc
      601 agaagtgcag cttacgacca tactttgcgc ccaaaagaat ttcgggaacg agtccgtttt
      661 cgaaagtgtc cggcgtgccc cattcgctgg tgacgagtgt atcgtggccg aggtgccacc
      721 acgcatcata cgcgagctgc tgtgggccgc ggtcgatctc ccagcgcccg aggatgtcaa
      781 agctttcgtg atccatcagg aagatgccgc cgggcgcctt gccttccgcg ttgccgaggg
      841 ccgcaacgta aattccatcc ggaccgcaat ggatcgtgtg tggccgcgta taaccggcct
      901 tctccgcaac ctcttccggc tcgatcaccc tgactatagc tggtttcgtc gggtcggcct
      961 tggtatcgag gatatgaatg cgcgatgacc tcaggccggg cacgaccaga tagcgccgct
     1021 cgacatgtgg gtgaggtgca ttcgggcaga gacaggacga acacgcgttc caaccgaagt
     1081 gatgcaactc gtcgccgaca ttcggcattg cgaccgtgtt taggatcgtt gagtagtgtt
     1141 tcgagtccgg gtcgaggtcc acgacggcga ttccgtcggg ggccttacgt gaagcatcaa
     1201 acgccgcgac ataagccagc ttctcggctg gcgccttcat ggccatgcgg ggtgagggat
     1261 agaaagtcgg atcgggtttc caggtgacca tgatgtgttc tcctctcgtt gcggatcctt
     1321 acttctctgc tcaggcggcg attgcgcact ccccgagtaa gctcgcgcta cagatcggcg
     1381 tcctgcggca gcgccgggcg cactgtgatg cggcttcggt tccccagctg caccacatcg
     1441 atcaagtgta ccggatagcg ctggaaggtg caggttatca cgcgacccga cagcagccta
     1501 cacatcgcgg tgagcggcgc cgggtggtct gcagttccct cgaacgattt ggattggctt
     1561 tggcgccgta atcgcgatat ttctgaacga tcgcggcgac cgctgggcgg gacaaaaaca
     1621 aagatgaaac gatgaatggt acggcccgct ttcgcgcccg tgatcgatct tccgaaactc
     1681 tcgaattcga ctatgtcgtc gtcggtgcgg gttcagccgg atgcgtgctc gccaaccgtc
     1741 tgagcgccga cggcaggcat tcggtgctgt tgctggaagc cggtccgaag gacaataacc
     1801 tctggatcca tgtgccgctc ggatatggca ggctgttcaa ggaaaaaacc gtcaactgga
     1861 tgtaccagac cgagccggag ccgggactcg acgggcgaac catctttcag ccgcgcggca
     1921 aggtgttggg cggatcgagc tcgatcaacg gcctgcttta tgtgcgcggc cagcaccaag
     1981 actatgatcg ctggcgtcag catggcaaca gcgggtgggg ttttgacgac gtcctgccgt
     2041 atttcaagaa ggcggaaaac caggagcgcg gcgccgacga tttccacggt gccggcggcc
     2101 cgctgccggt ttccgatctg gtacacgccg accgtctttc agcggctttc atcgcggcgg
     2161 cggccgagac cggcatcccc gtcaatcccg acttcaacgg cgcttcgcag gaaggcgctg
     2221 gattcttcca gaccacgacg cggcgggggc gcagggcgag caccgcggtc gcgtacctcc
     2281 gccccgccag gggacgcagg aatctgcagg tcgagacctc cgcgctggcg caacgcatcc
     2341 tgttcgatgg acatcgcgcc gatacgatcg aattccgccg ggccggcgtg ctgcgaacgg
     2401 cgcgggcgcg aaaggaaatc ctggtttcga gcggcgcgta caactcgccg caattgctgc
     2461 agctgtccgg cgtgggcccc gcggacctgt tgcgccagca cggcatcgat gtcgtgctcg
     2521 atgcgccggg cgtagggcac gatctgcagg atcatatgca ggtcagggtg gtcatgcgtt
     2581 gcacccaaag catcacgctc aatgacgtcg tcaacagccc ggttcggcgg atcttcgcgg
     2641 gcgcgcgcta cgcggccttt cgcaaggggc cattgacgat cgccgcgggc acgtcagggg
     2701 cgttcttcaa aaccagtccc cgcctcgcga ccccggatat ccagatccac tttttgccct
     2761 tcagcaccga caagatggga gaaagactgc attcgttttc cggcttttcg gcctcggtct
     2821 gtcaattgcg cccggagagc cgcggctcgc ttcgcatccg aagcgccgac ccgaccgcag
     2881 caccggaaat acggatcaac tatctctcaa ccgaggtcga tcgcaccgcc aacgtcgaag
     2941 gcctgaagat attgcgaagc attttgcggg cgcccgcgct tcgtccctat gtgatggagg
     3001 aggtcgatcc cggcgcaagg gtttccaccg atgaggagct gttgaactac tgccgcgcgc
     3061 gcggcagcac gatctatcat ccgacctcga cctgccgaat gggaagcgat cctcttgccg
     3121 tggtcgatca gcgcttgcgg gttcgcgggc tggaaggcct gcgcgtggtc gacggctcga
     3181 tcatgccgga tctggtgtcg ggcaatacca acgctgcgat cataatgatc gccgaaaagg
     3241 cgtctgacat gatcctggag gatgcgcggt agcgcggaac tcctggctgg tgtttgcatt
     3301 ccggcgctgg ctggttttat gttccggcca gaacctggcc gagattcccg cgcggcgttg
     3361 aaggagcatg agtggtggcc attcgaatac ggatcggcac gcgcaagagc gtgatggcgc
     3421 tggcccagac cgaggaaatt gcccgtcgcc tgaccgcggc cgatcccgat ctcgatgtcg
     3481 agatcgtcaa gttcgaaacg tcaggcgatt ccgatcagac cggcaggctg ttgcagcatg
     3541 gcgggaaggg cggcgcgttc gttgccgaaa tccgtgcggc cgttgtttcc ggcgaactgg
     3601 acgccgcgat gcattcgctc aaggacatgc cgggcaatga ggacacgccc ggccttgtga
     3661 tcggcgcgac gcttgagcgc gatccgcccg gcgacgcgct ggtgctgcga tcaggcgttt
     3721 cgatcgacga tctcaggcgc gcgcgcggca agggtttcaa gatcggcacc aatgcggtgc
     3781 ggcgcgcggc ctatgcgcgg cggctgtttc cggacatcga ggtgattcac tatcgcggcg
     3841 ccgccgacac ccgcgttcgc aagctcgaca acctggaaat gcagcggctg ccggatggcg
     3901 gtgcggtcgg tccggcggac gcgctgatca tggcgcgctc ggggctggaa cgcgtcggcc
     3961 tggccggtcg gatcgcctat gaattctcga tacgggaaat gctgccggcg gtggggcagg
     4021 ggatcgtcgc ggtagagtgt gcgatcgatg actggcagac ccggcgcatg ctggcacgga
     4081 tcgacgatcc cgccgcgcac caaagcgccg atgccgagcg cgaggtgctg tgggtcctca
     4141 acggccactg caactcgccg atcgcgggat tttcaaccat cgacggggcg cagatgtcgc
     4201 tgaccgcgtc ggtgctcgat gaagcgggcg gactgttcat cgaagccacg cgatccggcc
     4261 ccgccaaccg cccgcgcgaa ctcggccgtg cggtcgggtt cgacctgctc gccaagggcg
     4321 cggccgagat catcgagaga agtcggccgg ggtgatgtgt ccagcccctc ccacgagggg
     4381 agaggggaag agcacgcatt cgctcaacct tcacccctga tgcgtccgca cccgcttgat
     4441 catctgctcg acatgcgcga tcggggtttc cggctggatg ccgtgaccga ggttgaagat
     4501 caatctgccg ccggcgtagt tttccagcac gtcgtcgatg gcgcgatcca gcgcagcacc
     4561 gccggcgatc agcaccagcg gatcgagatt gccctgaacg gcgacgcggc tctgcacgcg
     4621 ctcgcggatc agtgacggct ccgcggccca atcgatgctg acggcatcga cgccggtggc
     4681 gtcgacgtaa cccggcagca gcgcgccggc gcctctggga aagccgatga ttttcgcgtc
     4741 cggcattttc gcacgtacgc cttcgacgat gcgccgcgtc ggctcggtcg accagcgcgc
     4801 aaattcgcgc ggcggcaaca cgccggccca ggtgtcgaag atctgcagca cgtcggcgcc
     4861 agccttgagc tgtcccagca gatactggat cgagttctcc accagcacgt cgatgattgt
     4921 ggaaaacgct tccgggtggc gataggccat catccgcgcc ggcgcctggt ccggggtgcc
     4981 ttgccccgcc accatatagg tcgccaccgt ccacggcgcg ccgcaaaagc cgatcagcgc
     5041 gatgttcgga tcgagctcgc ggcgtacccg gcgcagagcc tcatagaccg gctcgagttt
     5101 gctgaagtcg ccgtgccgcg ccagcgtccc gacttgctcc ggcgtctcca ggggatcgag
     5161 ccgcggccct tcgcttacct cgaacctgac ctcacgaccc agcgcgtagg ggatcacgag
     5221 aatatccgag aagatgatcg cggcatcgaa gttgaagcgg cggatcggct gcagcgtcac
     5281 ctcggcggca tattccggcg tgaagcacag atcgagaaag ccgcccgccc tgcagcgtaa
     5341 ttcgcgatat tccggcagat agcggccggc ctgccgcatc atccagatcg gaggtactgg
     5401 ctgcctgcgg ccggaaagca cttcaaggaa gggttttagg gcatgaatct gggtcaaatg
     5461 cggttccgca ggggcgatcg agagggcgtt gccgctcctg atacactgcg gatatggatt
     5521 tggccaagcc gcgattatcc gggcccgaac gatcgcggcg atcgctcgcg ggaaacatca
     5581 ggtcgtcccg gcgttcaaga ccttctcgcg cagtcctccg gcctggcgat cgggtctgcc
     5641 gggcgctcca aatgcgaaat tccaaacggg ctgggatcgg aattttgtct ttggagcatc
     5701 gcaagctcga taagggtgca atttcatcga cgagacattc gaccccaagt tgcgccagtt
     5761 tgtctgtgca gtattgggca tagtgcaaaa aagattggtt ctatgcccgt ttccgggcat
     5821 tgcggcgccg aattcgtggg cgcgaccggg gtcagttcca acaaccctga tgatgttccg
     5881 ctcctgtttg tttgaaacga ccggagtgct tgtttggaac gtccggagtg ccgacgcgtt
     5941 agcttccgac ggagacaaaa catggccgag aaattcgatc cggcggcgca cgacaggcat
     6001 gccgccaatc cgcgcgaagc cgcagcggcg gaccgcgaaa tccacgcgaa actggaagcc
     6061 ggtctgatcg acagttttcc ggcatccgat ccggtcagcg ccgcacaacc ggcaccctcg
     6121 aaggcggatg gcgatcgcga aggcacctcg ctttgggcca aggtccgagc ggtgttccga
     6181 tagattacgg gctcgatctg gacggatcgc cggctgcgct tccgaagtgc gccttgatga
     6241 aacacgagtt tgatcacgcc gccaacgact cgccgcgcag cggcagttcg aagcaggtga
     6301 cgccgttgct atggggcgcg attgtcgtcg ccatcgtggt gctgatcctg tggtttgcga
     6361 tcaaggcttg aggctccagg ctttgtgccg tgaacgggtt tcgcacggga accattcaac
     6421 tcagcgggaa ttgcctcgcc gatggttcac aaactgagga gcgaaggatg aaacgttttc
     6481 ttgcgatggc cgtcgccgca gctgcgattt gtgtcgcaat gccggtcggc gcggaagaaa
     6541 tcggagttgg cgtgggtccg gacggcgtga ccgtcggaac cgtccgtggt gaccgtgacc
     6601 gtgacaggga tcgcgatcga gaagtgatcc gggagcgtga ccacagggat cgcgataccg
     6661 ttgtgatcca gagggggcgc gatcatgatc gtgactacga ccgcgatcga aaggtgatca
     6721 tcgaccgcga ccggtagtca tcatgctaat aatcacgtcc ggcttgatta agcggccccc
     6781 cttgcggagg ccgcgccttt tcaataatgc gggggcggtt cgttcgcagc gccagggctg
     6841 tgagtctcgg cctcctgcag gcgctcgctc aagttggcaa tctgccgcgt cagcgcgtcg
     6901 atttgttgcc actgcgcggt gatggtctgg ttcagcgtct cgatggtttc gtcctgaaac
     6961 gccagccgcg tttcgagcac atcgatgcgc tcgctcagcg ccttgatgtc actcatcgga
     7021 atcctgttcg gtccggcgct gccgtaatcc gtggccgagc gcgacgcggt cgtcgaacac
     7081 aaaacattcg ccacgccagc ggctctcggc ttgcggcacg ccctcgagat atccgaggat
     7141 gccgcccttg aggtgataga cctgcgcaaa accgcgggcg agcagatagg cgctggcctt
     7201 ctcgcagcga atgccgccgg tgcagaacat cgcgatcctc tggtgtttcg ccggatcgag
     7261 atggcgcgca gcaaaatcct tgaattgccc aaaactcctt atgccgggat cgacggcgcc
     7321 gggaaatgtg cccatcgcca cctcgaaggc gttgcgggtg tcgatcacca gcgtatcagg
     7381 acccgcgatc agcgcgttcc agtcgccggg ctcgacatac acgccgacct ggcgggtcgg
     7441 atcggcatgg cggtcgccga gggtaacgat ctcctttttc agccggatct tcaggcgttg
     7501 aaacggcatc gccgcggcat gcgagaattt caattcgagg ttgtcgagcc ggccatcgaa
     7561 cagagcgccg cattgcaatt cttcaaccag tgcggcaatc gcctccggct ggcccgccag
     7621 cgtgccgttg atgccttcat gggccagcag cacgctgccc ttcagcccaa gtccggcgca
     7681 gaccgcgcgc agcggctcgc gcagctcccg gaaatccggc aacgcggcga attgatagaa
     7741 agcagcgacc ctgtgcggca tcacggtggt ttatcagcag ggagggggag aggaaacccc
     7801 ggtgtcgtcc ctgcgatcgc agggacgaca ctgatatgag ttatgcgccc ccggccattg
     7861 cccatcgctg catgaatatg ccatgtaacc cggcccctcg ggcacaacgt aagatgacga
     7921 aagtgagcaa tttggcatga ggaatttcca tctcgccggc cggtcgacgg tccacgccca
     7981 gaatgcgatg gtggcgacgt cgcatccgct ggcgtcattg acggcgatcg aggtgctgcg
     8041 ttccggcggc actgccgcgg atgccgccgt ggcggcctgc gcgctgcttg gcgtgatcga
     8101 gccgcaatcc accgggatcg gcggggattg cttcgcgctg cttcagcgca agggcgaggg
     8161 caagataatg gcctataacg gttccggccg cgcgccgcgg gcggccgacg cgtcgtggta
     8221 tctcgcgcgc aacatgcatg cgatcccgct gacgtcggcg cacgccgtca gcattcccgg
     8281 tgcgatcgat gcctgggtga ccatcctgcg cgatcatggc aagcttgggc tcgacagcct
     8341 gctgcagccc gcgatcaagg ccgcggaaga gggctatgtc gtagcccccc gcatcgcctt
     8401 cagttggaag aacgagttcg acaagttgaa aaaaggcacc aacaccgagc gctatctgct
     8461 cccgcacggc aaggcgccgg tcgccggcga cgtcatccat cagcccgaac tcggccagac
     8521 cttgcgggcg atcgcgagcg gcggccgcga cgcgttctac aagggtgcga tcgccgaaga
     8581 catggtcgag actttgcgtg gcatcggcgg cctgcatacg ctggacgatt tcgccgcgca
     8641 caccaccgaa accacgacgc cgatcggcac cgtgtacaag ggccaccatg tctggcagtg
     8701 cccgccgaac ggtccgggca tcaccatgct ggtgatgctg aacatcctgt cgcgtttcga
     8761 tctgacgaaa tttccgccga tgagcgtcga gcggttccat ctcgaagcag aagccgcgcg
     8821 catcgcctac atgatgcgcg agcagtacat cggcgatccc gcttttgtcg acgtcgatgt
     8881 ggtcaggatc ctgacgaagg aattcgccga ggagtacggc agcaagatca ggatggaccg
     8941 gttgctcgat ctgccgaatg tgtcgccgcc gatgaacccc tcgacggttt acgtcaccgt
     9001 ggtggaccgc gaccgcaacg tctgttcatt catcaattcg atcgcgcacg cgttcggttc
     9061 ggcgatcgtc tccaacaaga ccggcattct gctgcaaaat cgcggcggcg gattccgggt
     9121 tcagcccggc catcccaatt gcatcgcgcc gggcaagcga ccgctgcata cgatcatccc
     9181 aagccttgtc accaaggccg gccgggcggt gatgccgttc ggcgtgatgg gcgggcagta
     9241 ccagccggtc gggcaggtgc atgtgctgac caacgtgctc gactacggct gcgacgtgca
     9301 ggaggcgatc gacatgccgc gcggcctgca ttacgaaggc gtctaccagc tcgaggatgg
     9361 cgtgccggcc aatatcgtcg atggcctgag gaagctcggg catcagaccg cgagcctcgt
     9421 cacgccgcac ggcggcggcc aggcgatctg gatcgactgg gacaagggca cgctcaccgg
     9481 cggttccgat ccgcgcaagg atggctgcgc gctcggctat tgaaccaacc gtctgcgcat
     9541 ggggctttcc gtccctgtta cgaaggaatc tgaaagatgc gacataacat gtttcgaaat
     9601 cttccaatcg ccgttgccgt tgctggcgtc cttctcatcg caatgccgcc aagtgtatcg
     9661 gcccaaaccg caggaaaaac catgaccaca ccatcaggct tgcagatcac cgactctacg
     9721 gtcggcaccg gcgccacgcc aaaacccggc cagatctgcg tcatgcatta cacgggttgg
     9781 ctctacgagg gcggcaagaa gggcaagaag ttcgacagtt cggtggatcg caatgagccg
     9841 ttcgagtttc cgatcgggca gcgcaaggtg atcgccggct gggatgaagg cgtcgccacc
     9901 atgaaggtcg gcggcaagcg caccttgatc atcccgccgg cactcggcta cggcgcccgt
     9961 ggcgccggcg gcgtcattcc gccgaatgcg acgctgatgt tcgacgtcga attgcttgac
    10021 gtgaaggggt aactgtctgt tcgcggccga aaagttctgt gccggggaaa cgcatcgtgc
    10081 agatttccat ccttgatgac tatttcgaca ccctgcgtac cctgaaatgt ttcggcaaac
    10141 tcgcaggaca tgacgtcacg gtctggaacg atcacgtcca ggacgtggaa acgctggcgg
    10201 agcggctctg cgacaccgag gcgctggtgc tgatccgcga gcggacccaa attcgcaacg
    10261 ccctgctgga gcgattgccg aagctgaaat tgatcagcca gcgcagcgtc tatccccaca
    10321 tcgacatcga cgcctgcacc cggctcggca tcatcgtgtc gtccagccag cacgccgata
    10381 cgccgtctta cgccacggct gaattcacct gggggctggt gctggcggcg atgcgccaga
    10441 ttccgcagca gatggcggcg ctgaaagccg gcaattggca gatcggcgtc ggccacacac
    10501 tgcgcggcaa gacgctcggc atctacggct acggccggat cggcgcggtc gtcgccggat
    10561 atggcaaggc gttcggcatg gaggtgctgg tgtgggcgcg taagccggcg ctggcgaagg
    10621 cgcgcgccga cggttacgcg accgcgagca gcaaggctgc atttttcgag caatgcgatg
    10681 tactctcgct gcacatgcgc ctggtcgacg cgacgcgcgg catcgtgacc gcggctgatc
    10741 tcgcgcgcat gaagccgacc gcgctcctgg tcaacaccag ccgcgcgcca ttgatcgaac
    10801 cgaacgcact ggtgaacgcg ttgcgcgccg gccggcccgg catggcggcg gtggatgtct
    10861 atgagaaaga gccgctgcgc gataccagcg atccgctgct caacatggac aacgtcgtcg
    10921 ccacgccgca cctcggctac gtctcgcgcg acgaatacga aatccagttc acggatattt
    10981 tcgaccagat caccgcctat gccaccggta cgccgaccaa tgtcgtcaat cccgacgcga
    11041 taggaaaacg gtgagcgcat ttaaacgaga aagaccagcc caagggaccg gtcttcgact
    11101 tcaaatcgac cagcgtcgat cagagtttct tgtttgccgc ggcggcggtc ttgttggtgg
    11161 cttccttggc gtcgcccatg gccttctggc ccttgccttt gatttcctga atggcgcctt
    11221 cgccctgcat cttgtcggaa ccgacggctt cgccgacgcc ccgcttggcc ttgccgatgg
    11281 cttcgttggt ggcgccttta accttgtctg ctgttgcacc catgggaact ctcctttgcc
    11341 agggttaagc ttcgtcaaga caaacctcaa atgcaccgaa tattcctgat cccggctggt
    11401 ctgcgatcac agtattatgt accttttaca gcgaacaatt ttctcggaaa gcagcacgat
    11461 gacgggccct cacgatcata cccatgccga ccattcacac gatcacgacg acccggcgcg
    11521 ttggaagcat gatggcgtcc gcgttattcc cggcgaccag ctcgacccca acgtgccctc
    11581 gaccgccggc atggaccgca aggccgcgat caattttgcc cgcgtcggtg cgcagaaatt
    11641 atgggcgggc accgtgacca tcaagccgga tgccaagacc ggcgcgcatc accatggcca
    11701 tctcgaaagc atcatctatg tcgtgaaggg caaggcgcgg atgcgctggg gcgagcatct
    11761 gcaattcatg gcggaagcca atcccggcga tttcattttc atcccgccct atgtgccgca
    11821 tcaggaaatc aacgccagcc ccgaccaggt gctggaatgc gtgctggtgc gcagcgacgg
    11881 cgaggcggtc gcgatcaacc tcgacatcga gccggtggaa aaaccggaaa cggtgctgtg
    11941 ggtcgatccc gtgcaccgcg atcccgcggc gacgaagtga cgacgtcatt ccgggtctgg
    12001 tccttcggac catcccggaa tgacggcctc tattacgcaa acaccttgtg atccatcgtg
    12061 ccgttcggca ccacatagcc gtagcgcgtg tttgagggct cggggccggc cttctcgtat
    12121 gccgccttca tcacggcgaa gcgatcgccc ttgagctcga gcatcgcgcg cttcggctgg
    12181 atatcgtaga gccgcgcatt gttgccgccg aagatcgctg atttgaccgg tccgtcgacg
    12241 gcgccgagcg gggcgtagcc gaatttcttc tgcatggcct ccgggatttc cagacggcgc
    12301 aggccctcga tctgccattg cggcgccccg gtccacagcg cgtcggtgcc ccagcagacg
    12361 tgatcgacgc cgagcccttt gatcagcgtg cccatcaggg ctgcgcagac gtttggctcc
    12421 gacaccagcg tggtcgcgaa cagttgcccg acgtcgccat agacattgtt gacgccgtac
    12481 tgggctggaa tgtcggcgag atcgctggtc caggaaatcc gcccggtgcg ctcgaactcg
    12541 gccagcgcct gtttcgggtc gccgccgaca tggcgatagg ccgagtggta aatgacaaaa
    12601 ttgagctgcg gccagtcctt cgcggcctga ccgacatcgg cgacgtcggc gaagccgcgc
    12661 agattgggat attgcttttc aacgcccggc gcgaacagac ccttgtggat gcagacattc
    12721 ttgatgccgg ccttcaccat cttctcgtag cccttgtagg cgaccttctc gtcgtccatc
    12781 cgccagggat agcggctgat ctccttgtgg gtgttgtcgc cgacggtgta gcccttgcag
    12841 gattcaggtt tcagcgccaa tcccgcgtcg agcttgtcga gccagccggg ctgtcccggc
    12901 gtgaagatca tgtggcagaa tacccggcgc gagccggcct cgtcgttgat cgtcttgcgc
    12961 gcgtccgcca tctgctcgtt ggtcaggaac cagtcctgct cgatgtcgga gggggccgag
    13021 gagatcagcg agatcttggt gtcgctgtcg aggaacatct cttttttgta gttgttgaat
    13081 ttcagatcct cgatggtctg ctcgtggtcg gccagttgct tgttccagcc ggccttgccc
    13141 accgccttgc gcatgtcgac gaaggtggtg atgcgggtgt cgtcgcgcag gaaatgcgtg
    13201 tgcatgtcca tgatgaactg gtccttcagc gcgccggcgc gctcctcggc catcgcgggc
    13261 gtagcggctt cggcgcgcgt cacgtcgaac agcggcccgt agacttcgtt catcgcgaca
    13321 aaggatgcgg ccatgccggc tgcactctgg aaaaaccggc ggcggtccag gccctgcttg
    13381 ccgccgagat cgtcggccat cgccagcagc cgggactcga cttcgcgttg ccgctcgttc
    13441 tgcgggtcgg gataaaattc gtcgctggcc acgatctggg tcgggatcgg cgtcttgtat
    13501 tggcacaatt cggacggcat cagcgcggca agttctgcgt cggtcagttg gttgctcact
    13561 gttcttgctc cctgggttgt tctccgcggt taaagtccct cggttgacga ctgaaagact
    13621 agacctctct ttggcgggag caaggctgtt gcgcgcttgg ccttctaccg gttgcgcatt
    13681 gcagcgaagc gtgacttccg cgaaacgccg gggtttgcta caacgcggac gaactttctt
    13741 gggaattcga acggttaccg ggcgcgtcgc ggcgtcgctt aagacctgca aaaaaatcag
    13801 gcagtctgat tgggagaaaa tcatggaaaa attcgatcgc ccgctggcgc ggcgcacggt
    13861 catcaggggt gccgggcttg gcttggttgc cggcggcctc gcgagttcgc tgcccgcgcc
    13921 aagcacgagc gcggcggctg ccgagggcgg cgagatctgg agcagcgaat actgggcgaa
    13981 gaagggcgat gtcccgcttt ggatgtaccg caaacgggta ggggcgccca aagccggcga
    14041 gcctgcgcgg ccggtcgtgt tctttgtcca cggctcgtcg gtgacttcaa gggtgttcga
    14101 tctcaaggtg cctggccgcg gcgagtattc ggtgatggac gagttcgcgc gctatgggtt
    14161 cgactgctgg accatggatc atgaaaacta tggcaagtcg gggcgcacct ccggcaattc
    14221 cgatatcgcc agcggcgttg aggatctgaa ggtcgcggct gaaatcgtca cccgcgaaac
    14281 cggtcagaag agatttcatt tcgtcggcga atcctccggc gcgctgcgcg ccggcgccta
    14341 tgccatggcg gcgcccgagc gcatcgaccg gctggtgttt gccgccttta cgtataaggg
    14401 cgagggatcg ccgaccctga ccaaacgcgc cgagcagctc gactattacc gcacccacaa
    14461 catgcgcaag cgcgatcgcg agatgatccg ctcgatcgcc acccgcgaca agccgggcac
    14521 cagcgatccc gccgccgtcg aggtgttggc ggatgtcgag atgcagttcg gcgaccagat
    14581 cccgaccggc acctatctcg acatgacggc caatctgccg gtggtgcacc ccgacaaggt
    14641 gctggcgccg gtgctgttgg tgcgcggcga atatgacggc atcgccaccg tcgccgacct
    14701 cgaggaattc tataatcagc tgccgaacgg cgaccgccag ttcattatcc tgccgggaac
    14761 cgcgcattcc gttgcgcttg ccaccaaccg gcaattgttc tggcacgtga cccgggcgtt
    14821 cctcaccatg cccgc
//
LOCUS       DATNOI010000558         2125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1816260,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000558 DATNOI010000000
VERSION     DATNOI010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816260"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1728)
                     /gene="sufB"
                     /locus_tag="VN968_22525"
     CDS             complement(217..1728)
                     /gene="sufB"
                     /locus_tag="VN968_22525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HXL32045.1"
                     /translation="MPAVQETVERVRLIDVDQYKYGFETLIESEKAPKGLNEDTVRFI
                     SAKKNEPAWLLEWRLGAYRHWLTMREPKWARVDYPAIDYQDMYFYAAPKQAVAPKSLD
                     EIDPEILRTYEKLGIPLREQELLEGVVRRPDGEEGGGAVDPARRVAVDAVFDSVSVAT
                     TFKHELKKAGVIFMPISEAVREHPDLVQKYLGSVVPTSDNFFATLNSAVFSDGSFVYV
                     PPGVRCPMELSTYFRINERNSGQFERTLIIADKGSYVSYLEGCTAPRRDENQLHAAVV
                     ELIALDDAEIKYSTVQNWYPGDAQGRGGIFNFVTKRGLCRGNRSRISWTQVETGSAIT
                     WKYPSCILRGDHSRGEFYSIAISNGRQQVDSGTKMIHLGRNTTSRIISKGIAAGHSNN
                     TYRGLVSAHRRAVGARNFTNCDSLLIGNHCGAHTVPYIESKNPAAVFEHEASTSKISE
                     EMLFYCMQRGLSQEDAVALVVNGFVRDVLQQLPMEFAVEAQKLISISLEGSVG"
     gene            complement(1828..>2125)
                     /locus_tag="VN968_22530"
     CDS             complement(1828..>2125)
                     /locus_tag="VN968_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HXL32046.1"
                     /translation="ERLPNTSLFAAPGLRAETALINLDLMGFAVSSGSACSSGKVAAS
                     HVLAAMGVPGDLSAGAIRLSIGPATRENEIDLFLKAWMKLLAGLSKGGKSGLAA"
BASE COUNT          400 a          648 c          645 g          432 t
ORIGIN      
        1 cacgtaaacc tcttcttgga tttcttcgcc catcagaaag gcgcggctag aagcctgttc
       61 cggccatatc gaaccacttt ccggaatcat ggccgggctt gtcccggcca tctacgtctt
      121 gcttgcttgc agcgccgcaa gaaagacgtg gatgcccgcg acaagcgcgg gcatgacggc
      181 ggagggatga ttcggtctca ttgggacgcg ctctacctat ccaaccgagc cctcaaggct
      241 gatcgaaatc agcttctggg cctcgacggc gaattccatc ggcaactgtt gcagcacgtc
      301 gcgcacaaag ccgttgacga ccagcgccac cgcatcctcc tgcgaaaggc cacgctgcat
      361 gcaatagaac agcatctctt cggagatctt cgacgtcgac gcctcgtgct cgaacacggc
      421 ggcggggttt tttgactcga tgtacggcac cgtatgggcg ccgcagtgat tgccgatcag
      481 gagcgagtcg caattggtga agttgcgcgc gccgaccgcg cggcggtgcg ccgagaccag
      541 gccgcggtac gtgttgttgg aatgcccggc cgcaatgccc ttggaaatga tccggctcgt
      601 ggtgttgcgg ccgagatgga tcatcttggt gccggaatcg acctgctggc ggccattgga
      661 gatcgcaatc gagtagaact ctccgcgcga atggtcgccg cgcaggatgc agctcgggta
      721 tttccaggtg atcgccgagc cggtttcgac ctgggtccag gaaatcctgg agcggttgcc
      781 gcggcaaagg ccgcgcttgg tgacgaagtt gaagatgccg ccgcgcccct gggcgtcgcc
      841 cggataccag ttctggaccg tcgagtactt gatctccgcg tcatcaagcg cgatcagctc
      901 gaccacggcg gcgtggagct gattctcgtc gcggcgaggg gcggtgcacc cctccagata
      961 actcacatag gagcccttgt cggcgataat gagggtgcgc tcgaattggc cgctgttgcg
     1021 ttcgttgatg cggaaatagg tcgacagctc catcgggcag cgcacgcccg gcggcacata
     1081 cacgaaggag ccgtcggaaa acaccgccga gttgagcgtg gcgaagaaat tatccgatgt
     1141 tggcaccacg gagccgagat atttctgcac caggtccgga tgttcgcgga ccgcttcgga
     1201 gatcggcatg aagatgacgc cggccttctt gagctcatgc ttgaatgtgg ttgcgaccga
     1261 taccgaatcg aacaccgcgt caaccgcaac ccggcgggcc gggtcgacgg cacccccgcc
     1321 ttcttcgcca tcagggcgac gcaccacgcc ttccaacaat tcctgctcgc gcagcggaat
     1381 gccgagtttc tcgtacgtcc tgaggatttc ggggtcgatc tcgtcgaggg atttcggcgc
     1441 cacggcctgc ttcggcgcgg cataaaaata catgtcctgg taatcgatcg ccggatagtc
     1501 cacccgcgcc catttcggct cccgcatcgt gagccagtgg cggtacgcgc cgaggcgcca
     1561 ctcgagcaac caggcgggtt cgttcttttt tgcggagatg aagcgtacgg tgtcttcgtt
     1621 gagccccttc ggggccttct cggactcgat aagcgtctca aatccgtact tgtactggtc
     1681 cacgtcgatc aggcgcaccc gctcgacggt ctcttgcacg gccggcattt attccctccg
     1741 ctgcggtttc aaggaccgcg ggttggattg atctggtcgt ctatggtcgg ttctaaggtt
     1801 ccggcgatca cccatagctg gttgcggtca tgcggcaagg ccgctttttc ctcccttaga
     1861 tagccccgcc aggagcttca tccaagcctt aagaaacaaa tcgatttcat tttcgcgggt
     1921 cgccggcccg atactcagcc ggatcgcccc cgcgcttaaa tcccccggca ctcccatggc
     1981 ggccaggacg tgggaggccg cgactttgcc ggatgagcac gcggaacccg acgacacggc
     2041 gaaacccata agatcaaggt tgataagagc cgtttctgca cgcaatccgg gtgcggcaaa
     2101 caggcttgta ttgggcaatc gttcg
//
LOCUS       DATNOI010000559         4294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_945604,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000559 DATNOI010000000
VERSION     DATNOI010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945604"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VN968_22535"
     CDS             complement(<1..515)
                     /locus_tag="VN968_22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL32047.1"
                     /translation="MSRVMLLCGQLLVAVIVLGLWQLLATVPIFGRVLLPPFFFSNPI
                     DVGSQIVDWFASGVIWKHLAITLWESILAFAIGSLAGVAVGFWFARQPRVAAVFDPYV
                     KMANALPRVVLAPIFTLWLGLGIWSKVALGVTLVFFIVFFNVYQGVKEVSTTVLDNGR
                     MLGMNERQLTR"
     gene            complement(693..1259)
                     /locus_tag="VN968_22540"
     CDS             complement(693..1259)
                     /locus_tag="VN968_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HXL32048.1"
                     /translation="MFSRGSILAGILVLASSFGAHDALAQGVFTLSSPGFKDGERLAT
                     KNAGNNKANPNCVGDNVSPALSWANPPEGTKSYALLMFDPEGRPPGGVSHWVSYGIPA
                     SVTGFAEGEVSQPSDKYVGGQSTMKLSNYFGPCTPPGAPHHYTFTLIATDLEPSALQP
                     GLTRDELIKALDGHAKGATGLIGTFSKP"
     gene            complement(1322..2143)
                     /locus_tag="VN968_22545"
     CDS             complement(1322..2143)
                     /locus_tag="VN968_22545"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32049.1"
                     /translation="MLAGETAEATAVVLDDATVAFPVADGRVYTAVEKARLRIAHGEF
                     VAIVGPTGCGKSTLLNVAAGLLRPASGTVRIFDAPLTGLNRQAGYLFQADALFPWKTA
                     IDNVAIGLEISGAPRGHALERAQGWLTAVGLGAFGNRYPHMLSGGQRKRVGLAQVLIR
                     DPKILLMDEPFGPLDAQTRQIMGNLLLELWNADRKAVLFVTHDLEEAIALADRVVIMS
                     AGPNARIIGDWRVPLARPRDIFEVRLQKEFHSLHHEIWSVLKAEVLKGYAQSGGG"
     gene            2268..3071
                     /locus_tag="VN968_22550"
     CDS             2268..3071
                     /locus_tag="VN968_22550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL32050.1"
                     /translation="MTGAPPLIAFAHVGKSFDGGRGAKPVVAVDDVSLDVAEGELLAI
                     VGGSGSGKTTLLRLASRLIEADSGSITIEGADVRAVDPVQLRRRIGYVFQSGGLFPHM
                     SVADNIGITPRLLGAPAAEIASRVDELLDLVRLDRAQHRDRLPQELSGGQRQRVGVAR
                     ALAARPCIVLMDEPFGALDPLTRDALGDDYRALHNKLGLTTVMITHDMTEAILLADRV
                     AVMHAGRLLAQGTPAQLSRSDDPYVGELLRTPRRQAERLNVLLPRGGPA"
     gene            3068..>4294
                     /locus_tag="VN968_22555"
     CDS             3068..>4294
                     /locus_tag="VN968_22555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HXL32051.1"
                     /translation="MSLFSDPRWSEALRHLPDYLGNHVRVSVTALALGLLVSLPLAVF
                     SRNRPVMRGALLGLASIVQTVPGLALLALFYPLLLALAALSLSWLGVGFSAFGFLPAV
                     LALALYSMLPVLRNTITGLHGVEPAILEAARGVGMTPHQSLFMVELPLALPVMMAGIR
                     TAAVWVIGTATLSTPIGQTSLGNYIFAGLQTQNWVFVLFGCLAAAVLALAVDQLLALI
                     ETGLRQRSRLVVAFGGVGIAVLVAATLMPAVTRSQSTYIVGAKTFAEQYVLSALIAQR
                     LRAAGLSASSREGLGSNVIFEALASGDIDVYVDYSGTLWANQFHHSDIKPRKELLDEL
                     KATLAAQKITLLGELGFENAYALVMTRKRAEELGIHSIADLASHTPTMSMAADYEFFS
                     RPEWVGLREAYGLSFRT"
BASE COUNT          752 a         1388 c         1387 g          767 t
ORIGIN      
        1 tgccgcgtca attgccgttc gttcatgccg agcatgcggc cattgtccag cacggtggtg
       61 ctgacctcct tgacgccctg atagacgttg aagaacacga tgaagaacac cagcgtgacg
      121 ccgagcgcga ccttcgacca gatgccgagg ccgagccaca gcgtgaagat cggcgcaaga
      181 acgacgcgcg gcagtgcgtt ggccatcttc acataggggt cgaataccgc cgcaacccgg
      241 ggctggcgcg cgaaccagaa accgacggcg acgccggcaa gcgagccgat cgcgaaagcg
      301 agaatagact cccacagcgt gatcgcgagg tgtttccaga tcacgccgct tgcgaaccag
      361 tcgacaatct ggctgccgac gtcgatcggg ttggaaaaga aaaacggcgg cagcaggacg
      421 cggccgaaga tcggcaccgt ggcgagcaac tgccacagcc ccagcacaat gacggcgacc
      481 agcaattgac cgcagagcag catcacgcgg gacatgaata acaatctatc tccgggtcgt
      541 cccggcgaaa tgccgggacc cataaccccc ggagtttatt gttgcagaag gtcactacac
      601 cgtcgtccaa tcgcgaagcc acggcgtcgg tcccggcgtt cgccgggacg acgctgaaaa
      661 atctctccac gcgaggagcg cgcgcttcgg tcttacggct tggagaacgt gccgatcaaa
      721 ccggtagccc ccttggcgtg gccatccagc gccttgatca gttcgtcgcg cgtcaacccg
      781 ggctgcaggg ctgatggctc aagatcggtc gcgatcagcg tgaaggtgta gtgatggggg
      841 gcgccgggcg gcgtgcacgg tccgaagtag ttcgacagct tcatcgtgct ttgaccgccg
      901 acatatttgt cgctcggctg cgagacctcg ccttcggcga atccggtgac cgaagccgga
      961 atgccatagg acacccagtg gctgacgcct ccgggcggac gtccttccgg atcgaacatc
     1021 agcagcgcat agctcttggt accctccggc gggttcgccc aggacagcgc cggcgatacg
     1081 ttgtcgccga cgcaattcgg attggccttg ttgttgccgg cgtttttcgt agccaaccgc
     1141 tcgccgtcct tgaagccggg cgaagacagc gtgaacacgc cttgcgcgag ggcatcgtga
     1201 gccccgaacg aggacgccag caccaggatg ccggcaagta tcgaaccgcg tgaaaacatg
     1261 aacgcctccc agttgatttg atgtccgagt gtaacctccg cggcatcgag atcaaggcaa
     1321 gtcaaccgcc tcccgattgc gcgtaaccct tcaacacctc agccttgaga acactccata
     1381 tctcatgatg aagggagtga aattcttttt gcaggcggac ctcaaaaatg tcgcgcgggc
     1441 gggccagcgg tacgcgccag tcgccgatga tgcgcgcatt gggccctgcc gacatgatca
     1501 cgacacggtc ggcgagtgcg atcgcctcct cgagatcgtg ggtgacgaac agcaccgcct
     1561 tgcggtcggc attccacaat tccagcagga gattgcccat gatctgtcgc gtctgggcat
     1621 cgagcggccc gaagggttcg tccatcagca ggatcttggg atcgcggatc aacacttgcg
     1681 cgaggccgac gcgcttgcgc tggccgcccg acagcatatg cggatagcga ttgccgaacg
     1741 cgccgagacc cacggcggta agccagccct gcgcccgttc cagcgcgtgg ccgcgcggcg
     1801 cgcccgaaat ctcgagacca atggcgacgt tatcgatcgc ggtcttccat ggaaacagcg
     1861 catcggcctg gaacagatag ccggcctgcc ggttgaggcc ggtcagcgga gcatcgaaaa
     1921 tccgcaccgt tcccgatgcc ggtctcagca gaccggcggc gacattgagc agcgtcgatt
     1981 tcccgcaccc ggtgggcccg acgatggcga cgaactcgcc atgcgcgatc ctgagccggg
     2041 ccttttcgac cgcggtgtag acacggccgt ctgccaccgg gaaagccacc gtcgcgtcgt
     2101 caagcacgac cgccgtcgct tccgcggtct cgcctgccag cacccgcttc cccccatgtc
     2161 gtggcctgtg gattaccgcc atcatcccaa caattcaacc gatcgcggca ataggcaagg
     2221 cccgcgtcgg cttggtgggt tggacgcggt gtgatagtcc caacgtcatg accggcgccc
     2281 cgccactgat cgcatttgcg catgtcggca aaagcttcga cggcggccgc ggcgcgaaac
     2341 ccgttgtggc cgtggacgac gtctcgctcg acgtcgccga gggcgagttg ctcgccatcg
     2401 tcggcggctc cggctccggc aagaccacgc tgctgcggct cgccagccgg ctgatcgagg
     2461 ccgacagcgg cagcatcacg atcgagggcg ccgatgtccg cgcggtcgat cctgttcagc
     2521 tgcgccgccg catcggctac gtgttccaga gcggcgggct gtttccgcat atgagcgtcg
     2581 ccgacaatat cggcatcacg ccaaggctgc tcggcgcacc ggcggcggag attgcgtcgc
     2641 gggtcgatga attgctcgat ctggtgcggc tcgatcgcgc acaacatcgg gaccgcctgc
     2701 cgcaggagct gtcgggcggc cagcgccagc gcgtcggcgt cgctcgcgcc ctggcggcaa
     2761 ggccatgcat cgtgctaatg gacgagccgt tcggcgcgct cgatcccctc acccgcgatg
     2821 cgctcggtga cgattaccgg gccttgcaca acaagctcgg gctgaccacg gtcatgatca
     2881 cccacgacat gaccgaggcc attctgctcg ccgaccgcgt cgcggtcatg catgccggcc
     2941 ggctgctggc gcagggcacg ccggcacagc tttccagaag tgacgatccc tacgtcgggg
     3001 agttgttgcg cacgccgcgg cggcaggccg aacggctcaa cgtgctgctg ccgaggggtg
     3061 gcccggcatg agtctgttct ccgatccgcg ctggagcgag gcgctgcggc atctgccgga
     3121 ttacctcggc aatcacgtgc gcgtcagcgt cacggcgctg gcgctcggcc tcctggtcag
     3181 cctgccgctg gcggttttct cgcgcaaccg gccggtgatg cgcggcgcac tgctgggcct
     3241 cgccagcatt gtgcagacgg tgccgggcct cgcgctgctc gcgctgttct atccgctgct
     3301 gctggcgctg gcggcgttgt cgttgtcgtg gttgggggtt ggcttttccg cgttcggatt
     3361 ccttcccgcg gtgctggcgc tggcgcttta ttccatgctg ccggtgctgc gcaacaccat
     3421 caccggcctg catggtgtgg agcctgcgat cctcgaggcc gcgcgggggg tcggcatgac
     3481 cccgcatcag tcgctgttca tggttgagtt gccgctggcg ctgccggtga tgatggcggg
     3541 catccgcacc gcggcggtgt gggtgatcgg tactgcgacg ctgtcgaccc cgatcggaca
     3601 gaccagcctc ggcaactaca tctttgcggg attgcagacc cagaactggg tgttcgtgct
     3661 gttcggctgc ctcgccgccg ccgtgctggc gctcgcggtc gatcaattgc tggccctgat
     3721 cgagaccggc cttcgccagc gcagccgcct ggtggtggcg ttcggcggcg tcggaatcgc
     3781 ggtgctggtc gcggcaaccc tgatgcccgc ggtgacgcgc tcgcaatcga cctatatcgt
     3841 cggcgccaag acttttgccg aacaatacgt gctgtcggcg ctgatcgcgc agcggctgcg
     3901 ggcggcggga ctatccgcaa gttcgcgcga gggtctcggc tccaacgtga ttttcgaagc
     3961 gctggcgtcc ggcgatatcg acgtctatgt cgattattcc ggcacgctgt gggccaacca
     4021 gtttcaccac agcgatatca agccgcgcaa ggaattgctc gacgaattga aagcgacgct
     4081 ggcggcgcaa aaaatcacat tgctcggcga actcgggttc gagaacgcct acgcgctggt
     4141 gatgacgcga aagcgcgccg aggaactggg gatccattcc atcgccgatc tcgcctcgca
     4201 tacgccaacg atgtcgatgg ccgccgatta cgagttcttt tcgcgaccgg aatgggtcgg
     4261 gttgcgagag gcctacgggc tgtcgtttcg cacg
//
LOCUS       DATNOI010000560        17937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1834580,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000560 DATNOI010000000
VERSION     DATNOI010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17937
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1834580"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VN968_22560"
     CDS             <1..484
                     /locus_tag="VN968_22560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HXL32052.1"
                     /translation="IACAVFPDGTTGAQIDTLARQFLWQAGIDFDHGTGHGVGSYLSV
                     HEGPARISKLGTTALLRGMILSNEPGYYKTDAYGIRIENLELVVAADIAGAEKPINAF
                     ETLTLAPIDRRLINLNMLSGEELSWLNDYHARVRHEVRPHVDEATKVWLDAATAPLSL
                     "
     gene            698..1984
                     /locus_tag="VN968_22565"
     CDS             698..1984
                     /locus_tag="VN968_22565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HXL32053.1"
                     /translation="MHGVTGKPPRAAQDNAALATSRMMLLLLVAMTGVAPISLYLLVP
                     ALPVLASTFGRDISIAQMTVSLYMVGIACSQIIMGPLSDRFGRRPVLLAGLGLMVAAS
                     AGCIFAETLPQLIAARFLQALGGATGMVVSRAIIRDLYSRDRISAMISLVIAVMMIAQ
                     MLSPLTGGLLETAFGWRAIFYIITAASLIIAVAIAFTLPETRRDRAEGSGGFRGDVGR
                     LFTSRAFIGYVLCQVLASQIIFAFAGGGPYIVVTQMGRSSAEYGAWFATTGFAYLIGN
                     LFCVRFAPRHSLEKLIWFGLALQFLGALLNLVWGIAGLNQAPSWLFGTQMLVMFANAF
                     VMSNSTAGAISVRPEAAGTASGAMGFLQMGVGALVSQFGAWLGGHFLTPVPLNIAIVA
                     LSAACASTMIFLVPRRNLVVSEALIEQAEEEESGLL"
     gene            complement(2853..5003)
                     /gene="ligA"
                     /locus_tag="VN968_22570"
     CDS             complement(2853..5003)
                     /gene="ligA"
                     /locus_tag="VN968_22570"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HXL32054.1"
                     /translation="MIAKSKAKTPPAVDTLTKAQAKVELKRLALEIESHDKHYYQEDA
                     PTVTDAAYDALRQRVNAIEARFPELVTSESPSQKVGARPSGRFAKVRHAVPMLSLDNA
                     FAEEDVVDFVGRIRRFLKLGDDGKIAFSAEPKIDGLSMSLRYEAGELVTAATRGDGAV
                     GEDVTANIRTLEDVPKKLKGRNVPDVCEVRGEVYMTKQAFLALNERQKAAGDTIFANP
                     RNSAAGSLRQKDPSITASRPLGFFAYAWGQMSEMPADSQSGMIHWFERCGFKTNPLTK
                     LCHSVEQLIAFHRQIEEQRSHLDYDIDGVVYKVDRLDWQERLGFVSRTPRWAIAHKFP
                     AERAITVLRDIEIQVGRTGSFTPVGKLEPVGVGGVIVQNVSLHNEDYIKGIGGDGEQL
                     RQGRDIRIGDTVIIQRAGDVIPQVVDVVADKRPKGAKEFHFPKKCPCPLHTDVVREET
                     ATGEEGARARCTGEFACPYQRIEHLKLFASRRAFDIDGLGEKQIEFFFEQGWVKEPAD
                     IFTLPARNNKIRLGEHEGYGETSVRNLFAAIEGRRRIALERFIFALGMRHVGDTTALA
                     LARGYGSWTAFHDACLKVANGDEEAIAEMDALDQIGETVIASIRAYFGESHNRGIVER
                     LTKEVTILDTEKPKSNSAIAGKTVVFTGALEKMTRDEAKAMAERLGAKVSGSVSKKTD
                     YVVAGPGAGSKLAEAKKHGVTVLTEDEWLAMIGA"
     gene            5157..5446
                     /locus_tag="VN968_22575"
                     /pseudo
     CDS             5157..5446
                     /locus_tag="VN968_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088373.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(5628..7316)
                     /gene="recN"
                     /locus_tag="VN968_22580"
     CDS             complement(5628..7316)
                     /gene="recN"
                     /locus_tag="VN968_22580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HXL32055.1"
                     /translation="MLARLSIRDIVLIERLDIEFSRGLAVLTGETGAGKSILLDAFAL
                     ALGGRGDSGLVRHGAEQGQVTAVFDVPRDHPASVILSDNGLDDTSSDDTHEMILRRVQ
                     LADGRTRAFINDQAISVQTLKAVGSTLVEIHGQHDERALVDASTHRRLLDAFAGLEED
                     VTAVEALWEARRAANTILDAHRAGMERAAREADYLRHAADELKALAPKDGEETTLAGR
                     RALMMQGEKIAADLREAQDAVSGNHSPVAALSAAVRRLERRAGNSPTLVEPAVKAIDA
                     AINALEEADQHLTAALIAADFDPSELERIEERLFALRAASRKYSTPVDGLAALAAKYA
                     ADVALIDAGADQLKKLEAAAAEAVGRYNSAAAKLSAARAKSADKLNKAVNAELAPLKL
                     DRAKFMTQIESDPKSPGPQGFDRIEFWVQTNPGTRPGPLMKVASGGELSRFLLAIKVV
                     LSDRGSAPTLVFDEIDTGVGGAVADAIGARLARLADKVQVMAVTHAPQVAARADHHLL
                     ISKDSLDKGKRVATRVNALAPDHRREEIARMLAGAEITAEARAAAERLLRAATA"
     gene            complement(7329..8231)
                     /locus_tag="VN968_22585"
     CDS             complement(7329..8231)
                     /locus_tag="VN968_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HXL32056.1"
                     /translation="MSAQRMTLELRKVPDGFRRIGRQLRLAAGLVVLAVPLSGCGTGA
                     LWDKFLAKDDTFVEEPADKLYNEGLYLMNQKKDAKAASKKFEEVDRQHPYSDWARKSL
                     LMSAYAFYNSGDYDSCIGAATRYVTLHPGSPDAAYAQYLIAASHYDQIPDTSRDQGRT
                     EKAIAALEEVIRKYPTTEYANQAKAKLEGARDQLAGKEMDIGRYNLEKRNYTGAINRF
                     KTVVTQYQTTRHVEEALARLTEAYMAIGVVGEAQTAAAVLGHNFPDSRWYKDAYNLVK
                     SGGLEPSENQGSYISRAFKKLGLG"
     gene            complement(8593..9552)
                     /gene="lpxC"
                     /locus_tag="VN968_22590"
     CDS             complement(8593..9552)
                     /gene="lpxC"
                     /locus_tag="VN968_22590"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HXL32057.1"
                     /translation="MKFSRQTTLRSQATVTGVGVHSGLPASLTLGPAPVDAGFIFVRT
                     GLDGRDRQVQASAESVISTEFATVLGNREGPLVSTSEHVLAALRGMGVDNATIEVDGP
                     EVPIMDGSAATFVAAIDQAGIVTLSASRRFIQVLKPVQVTMGASFGELRPHAAGFRAE
                     VEIDFTNPVIGRQNYKLDLSPERFRREICRARTFGCMNDVARLWSAGFSLGASFENSV
                     VFDETRVLNIEGLRYSDECARHKVLDVVGDLALAGLPLLGAYRSVRGGHKLNHAVLTA
                     LLADRSAWRVIEAETARRSRTHAEVGSGMVGGMIAPAYGPDVS"
     gene            complement(9905..11710)
                     /gene="ftsZ"
                     /locus_tag="VN968_22595"
     CDS             complement(9905..11710)
                     /gene="ftsZ"
                     /locus_tag="VN968_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXL32058.1"
                     /translation="MALNLTPPDISELKPRITVFGVGGAGGNAVNNMITAGLQGVDFV
                     VANTDAQALTMSKAQRIVQMGTQVTQGLGAGSQPDVGAAAAQEVIDELRDHLSGANMV
                     FVTAGMGGGTGTGAAPVIAKTARDMGILTVGVVTKPFHFEGQRRMRTAEAGIAELHKV
                     VDTLLIIPNQNLFRVANEKTTFADAFAMADQVLYSGVACITDLMVKEGLINLDFADVR
                     AVMREMGKAMMGTGEASGEKRALTAAEAAIANPLIDDSSMKGARGLLISITGGRDLTL
                     FEVDEAATRIREEVDQDANIIVGATFDENLDGVIRVSVVATGIEQALISRNANPTAAA
                     PASAVASSGTPDNRLADLTARLRADNQRLAERAQKLDPSAAAQVPSAALQQRASSGIE
                     RAALAAIAAAVAPEQAQLAASAPLQPASYGDVTVRPIAIAQKPTLFPDHGTGHVEAHQ
                     PPTSETFIPQAAERAPVRAPRMPKFEELPVPAQNEIRQARGEVEDDHPQKTRMSLLQR
                     LANVGLGRRDEETEPPIAARASGPAMAPIPPHAERKPQRAVAQQIGAGDPVSEYGRRP
                     APQGLDVHGRPAPVASAPQGDDHLDIPAFLRRQNN"
     gene            complement(11801..13123)
                     /gene="ftsA"
                     /locus_tag="VN968_22600"
     CDS             complement(11801..13123)
                     /gene="ftsA"
                     /locus_tag="VN968_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXL32059.1"
                     /translation="MTGLDRNQTPKTRPMPQKRTALVASLDIGTSKIACMIARLRPCP
                     PSDALRGRTHAVELIGYSQIQSRGVKAGAVVDLAECEQAVRHAVALAERMAKVRVESV
                     LLSVSGGRLHGQLVEAAADIRGGAVTSADVIRVTSTGMRHATGEGRTVLHALPVGYAL
                     DGVKGIRDPRGMVARQFGVDMNVVTVEATVAKNLMLVVERCHLNVEAMAASPYVAGLS
                     VLTDDEADIGAAVVEMGAGTTTIATYAGGRFVHASGFAVGGQHITMDLARGLSACIAD
                     AERIKTLYGTVLTGGSDARELMSVPAAGDNERDAPQIVSRATIANIVRQRAEEIFEMV
                     RDRLADSPFAAEPKARVVLSGGASQLSGTLELATQILGRPVRIGRPLGFGRLPSEAKN
                     ASFAVPSGLLVYPQYAHLEHVEPRHTRQLRTGTDGYFGRVGRWLREGF"
     gene            complement(13120..14106)
                     /locus_tag="VN968_22605"
     CDS             complement(13120..14106)
                     /locus_tag="VN968_22605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HXL32060.1"
                     /translation="MDGGGRLARSLGSPRPQTDLKAAAIGAAVLLRERLGYHRAGARA
                     RPEREQHPHRLIALLERYIPHRAGVAATVLMLLGSAGFGIVRGGHVEELSSALSDTRN
                     AIANSAGFRITAVGINGRKQLTQDEVLAIGGVNGRSSLLFLDAAAVREKLKANPWIGE
                     ATVLKLYPGQLQIDIVERSAFALWQQDGRLSVISDDGAVLERYMSQRFVSLPLVVGKG
                     ADTRARDFLALLARYPQVNAATKAAILVGERRWNLRLKDGLDIRLPEKDVGNALATLS
                     KLDKEDRLFSRDIVAVDMRLPDRLTVQLSEDAAKAREDLFKDKKPKKKAGDA"
     gene            complement(14046..14376)
                     /locus_tag="VN968_22610"
                     /pseudo
     CDS             complement(14046..14376)
                     /locus_tag="VN968_22610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562408.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
     gene            complement(14458..15444)
                     /locus_tag="VN968_22615"
     CDS             complement(14458..15444)
                     /locus_tag="VN968_22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HXL32061.1"
                     /translation="MRVTILFGGTNTERLVSVASAQAMHAALPEADLWFWDVSDTVHE
                     VQPQALRSHARPFEDAFKPGNRGIGLEPALDKAIAEERLLVLGLHGGRAENGELQAMC
                     EMRGIPFTGSGSASSHLAFDKVAAKRFAAIAGVTAPIGLMLDDIDAALAEYGRLIAKP
                     VRDGSSYGLIFVNAKQDLVAVRNAAKTEEYLIEPFIAGVEATCGVLEQPDGSLVALPP
                     IEIVPGEGAFDYTAKYLLKSTQEICPGRFAPEITAQIMDQAVKAHRAMSCSGYSRTDF
                     IISEKGPIFLETNTLPGLTKASLYPKSLKAQGIEFADFMQGQIALAKEHTHR"
     gene            complement(15444..16367)
                     /gene="murB"
                     /locus_tag="VN968_22620"
     CDS             complement(15444..16367)
                     /gene="murB"
                     /locus_tag="VN968_22620"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HXL32062.1"
                     /translation="MTFPDITPDLKAAMPQLRGRLLANQSLAELTWFRAGGPAQLLFT
                     PSDEDDLAYFLKHLPSEIPVYVVGVGSNLIVRDGGMPGVVIRLGPRGFGETSANRDIV
                     SAGAAALDKRVAEAAVAANIGGLEFFFGIPGTIGGALRMNAGANGAETKDVLVEARGI
                     GRDGRKQIFDNAGMKFIYRNSGVDPSIIFTAARFRGLMAAPEAIRVRMNEVQNHRETA
                     QPIREKTGGSTFKNPPGQSAWKLIDAAGCRGLRVGDAEVSEMHCNFLINTGAATGHDI
                     ETLGETVRARVKENSGIELHWEIKRIGIERT"
     gene            16388..16537
                     /locus_tag="VN968_22625"
                     /pseudo
     CDS             16388..16537
                     /locus_tag="VN968_22625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273016.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(16606..>17937)
                     /gene="murC"
                     /locus_tag="VN968_22630"
     CDS             complement(16606..>17937)
                     /gene="murC"
                     /locus_tag="VN968_22630"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HXL32063.1"
                     /translation="EVLSNLGYTVQGSDASESANVNRLRDKGIAITVGHTAENVDGAD
                     VLVVSTAIKRDNPELIAARAQRIPVVRRAEMLAELMRLKSCVAISGTHGKTTTTSMVA
                     ALLDAGNLDPTVINGGIINAYGTNARLGAGDWMVVEADESDGTFLKLPADVAIVTNVD
                     PEHLDHFKTFEAVQEAFRNFVENVPFYGFAVMCIDHPVVQSIVGNIEDRRIVTYGENP
                     QADARLVDLVPMGAGSSFKVVFRDRKTGMAHEIAGLMLPMAGRHNALNATAAIAVAHE
                     LGLSDDAIRKAIAGFGGVKRRFTRTGEWNGVTVIDDYGHHPVEISAVLKAARESTNGK
                     IIAVVQPHRFTRLQSLFEEFCTCFNDADTVVVAEVYPAGEAPIAGIDRDNFVLGLRAH
                     GHREVIALQKSADLARVVHGLAGSGDFVVCLGAGNITQWAYALPGELKALG"
BASE COUNT         3195 a         5721 c         5608 g         3413 t
ORIGIN      
        1 catcgcatgc gcggtatttc ccgacggcac caccggcgcg cagatcgaca cgctggcgcg
       61 ccagttcctc tggcaggccg gcatcgattt cgatcacggc accggccacg gcgtcggcag
      121 ctatctctcg gtgcatgagg gaccggcgcg aatttcaaaa ctcggcacca cggcgctgct
      181 tcgtggcatg atcctctcca atgagcccgg ctattacaag accgatgcgt atggcatccg
      241 gatcgagaat ctcgagctgg tggtcgccgc cgacattgcc ggcgccgaaa agccgatcaa
      301 tgcattcgaa acgctcaccc tggcgccgat cgaccgccgt ttgatcaatc tgaacatgct
      361 gagcggcgaa gaactgagct ggctcaacga ctatcatgcg agggtgcgcc acgaggtccg
      421 cccgcatgtc gacgaggcca ccaaggtgtg gctcgacgcc gcgaccgcgc cgctgtcgtt
      481 gtagtagtca ttccggggcg cgtccaagac gcgaacccgg aatctcgaga ttccccgatg
      541 tgcaattgca catctgaggt ctggtccttc ggaccatccc ggaatgacgg ggtcaattgt
      601 gcgcgtcccg aaaacggaat agccgcattc cgaaacggcg atgtcgctct ggcaggatag
      661 cgctacagtc tgattgacga ttttggatca gacctgcatg cacggagtga ctggcaagcc
      721 gcctcgcgcg gcacaagaca acgccgccct cgcgacatcc cgaatgatgc tgttgctgct
      781 cgtcgcgatg acgggcgtgg cgccgatttc gctctatctg ctggtgccgg cactgcccgt
      841 actggccagc acgttcggcc gcgatatctc gatcgcgcag atgaccgtgt cgctgtacat
      901 ggtcggtatc gcctgctcgc aaatcatcat gggcccgctg tcggaccgtt tcggacgccg
      961 cccggtgctg ctggcagggc tcggcctgat ggtggccgcg agcgccggct gcatcttcgc
     1021 ggaaactctg ccgcaactga tcgcggcgcg ttttctgcaa gcactcggcg gcgccaccgg
     1081 catggtggtc agccgcgcca tcatccgcga tctctacagc cgcgaccgca tcagcgccat
     1141 gatcagcctg gtgatcgcgg tgatgatgat cgcgcagatg ctgagcccgc tgaccggcgg
     1201 cctcttggag accgcgttcg gctggcgcgc catcttctac atcatcacgg ccgcatccct
     1261 catcattgcc gtggccatcg cgttcacgtt gccggagacg cgccgcgacc gcgccgaggg
     1321 cagcggcggg tttcgcggcg atgtcggccg tctcttcacc agccgcgcct tcatcggcta
     1381 cgtgctgtgc caggtgctgg cttcgcagat cattttcgcc ttcgccggcg gcggccccta
     1441 tatcgtggtg acgcagatgg gccgctccag cgctgaatac ggcgcgtggt ttgcgaccac
     1501 cggcttcgcc tatctgatcg gcaatctctt ctgcgtgcgc tttgcgccgc gccattcgct
     1561 ggagaaactg atctggttcg ggctggcgct gcaattcctg ggcgcgttac tgaacctggt
     1621 atggggcatc gccggtctca atcaggcgcc gtcctggctg ttcggcaccc agatgctggt
     1681 gatgttcgcc aacgccttcg tgatgtcgaa ctcgacggcc ggcgccatca gcgtccggcc
     1741 cgaagccgcc ggcaccgcat cgggcgcgat gggatttttg cagatggggg tcggcgcgct
     1801 ggtctcgcag ttcggcgcct ggcttggcgg ccatttttta acgccggtgc cgctcaacat
     1861 cgccattgtc gccttgtccg ccgcgtgcgc ctcgacgatg atcttcctgg tgccgcgccg
     1921 caacctggtt gtcagcgaag cactgatcga acaggccgaa gaggaagagt cggggctgtt
     1981 gtagagaggc gcgaagtgga gcactgcgcc gctccaattg gcgtacgcaa tgaactgcta
     2041 ccgattcaaa tttcaaacag cccggcttct gccgacacgg cttcgcgatc tcgcggccgg
     2101 atacgcccga gttttatctt gaatatcccg ccctcctcga tcagagggcg cagggaatgc
     2161 cgggcgcccg atgcgcccgc agccgcgtgt gcaatggtag tggtggaagg cacacgcgtt
     2221 agacaggtca caccggaatc acccggcatt ccccgcgcaa tggttttaac gggttccttc
     2281 gtgctctccc cggcgaccgg gctttcttgc caccgtcgcc tgcggacttg catccgcaaa
     2341 cttgacacca gcgtcggggt gtcaggacca cacgacttca ccgtccgcgt ccgcgccctt
     2401 cgtccgaaag cgcatccgcg tccaccggat cccgccccgc gtccgtgacg ttgcgcaacg
     2461 cccctctgtg ggacaggacg gggcgagttg tagatttgat ttgcccgacg ccgaaagcga
     2521 aatatttttg caaacaggac tggacagact agatccgcaa aacaaaactg atttgcccgt
     2581 caggcggcgc cgagcgcttg gtggcggatg ataacgaccg tatctgtggc gggcggggtg
     2641 catcacttcc ttccgccgag aagtggcgca ctgctctctt caacctcgcc ccgctctttg
     2701 cggggagagg tcggcgcgta gcgccgggcg aggggcgagg cgatggtaca gatacgaacg
     2761 aacgcctctc gggccggaaa cattcgaccc acaaattcgg aaaatgtatt tggcgccgtg
     2821 cctggcccct ctctccccgc gcgacgattt cctcacgccc cgatcatcgc gagccattcg
     2881 tcttcggtca gcacggtgac gccgtgcttc ttggcctcgg cgagctttga accggcgccg
     2941 ggaccggcga ctacataatc cgtcttcttc gacaccgagc cggacacctt ggcgccgagc
     3001 cgctcggcca tggccttggc ttcgtcgcgc gtcatttttt ccagcgcgcc ggtgaagacc
     3061 acggtcttgc cggcgatggc cgagttgctt ttgggctttt cggtatcgag gatggtgact
     3121 tcctttgtca gccgctcgac aatgccgcga ttatggctct cgccgaaata ggctctgatg
     3181 ctggcgatca cggtctcgcc gatctggtcg agcgcatcca tctcggcgat ggcctcctcg
     3241 tcgccgttgg cgaccttcag gcaggcgtcg tgaaatgccg tccatgaacc gtacccgcgc
     3301 gcgagcgcca gcgccgtggt atcgccgaca tggcgcatgc cgagcgcgaa gatgaaacgc
     3361 tcgagcgcga tccgccgccg tccctcgata gcggcaaaca gattgcgcac cgaggtttcc
     3421 ccgtagcctt catgctcccc gagcctgatc ttgttgttgc gcgcgggcag cgtgaagata
     3481 tcggcaggct ctttcaccca tccctgctcg aaaaagaatt cgatctgctt ttcgcccagc
     3541 ccgtcgatat cgaaggcgcg gcgcgaggca aacaatttca ggtgctcgat cctttggtag
     3601 ggacaggcga actcgccggt gcagcgggcg cgggcgccct cctcgccggt tgcggtctcc
     3661 tcgcgcacca cgtcggtgtg cagcgggcac ggacactttt tcgggaagtg aaattccttg
     3721 gcgcctttcg gccgcttgtc ggccaccacg tccaccacct gcgggatgac gtcgccggcg
     3781 cgctggatga tgacggtgtc gccgatgcgg atatcgcggc cctggcgcaa ttgctcgccg
     3841 tcgccgccga tgcccttgat gtaatcctca ttgtgcagcg agacgttctg cacgatgacg
     3901 ccgccgaccc cgaccggctc gagcttgccg accggcgtga acgagccggt gcggccgacc
     3961 tggatctcga tatccctgag cacggtgatg gcgcgttcgg cggggaactt gtgcgcaatc
     4021 gcccagcgcg gcgtgcgcga cacgaagccg agacgctcct gccaatcgag gcggtccacc
     4081 ttgtagacga ccccgtcgat atcgtaatcg agatgggagc gctgttcctc gatttggcga
     4141 tgaaacgcga tcagctgctc gaccgagtga caaagtttgg tcagcggatt ggtcttgaag
     4201 ccgcagcgct cgaaccaatg gatcatcccg gactggctat ccgccggcat ctcgctcatc
     4261 tgcccccagg cgtaggcgaa aaagccgagg ggacgcgagg cggtaatgga cggatctttc
     4321 tggcgcaggg aacccgcggc cgaattgcgc ggattggcga agatggtatc gccggcggcc
     4381 ttctgccgct cgttaagcgc caggaacgcc tgcttggtca tgtagacttc gccgcgcacc
     4441 tcgcaaacgt cagggacgtt gcggcctttc agttttttcg gcacgtcctc cagcgtacgg
     4501 atattggcgg tgacgtcttc gcccaccgca ccgtcgccgc gcgtcgcggc ggtaacgagt
     4561 tcgccggctt cgtaacgcag cgacatcgac agcccgtcga ttttcggctc ggcgctgaag
     4621 gcgatcttgc catcgtcgcc cagtttcaga aaacgcctga tgcggccgac gaaatcgacg
     4681 acgtcctctt ccgcaaaggc attgtcgagc gacagcatcg gcaccgcgtg ccggaccttg
     4741 gcgaagcggc cggaaggtct tgcaccgact ttctgcgagg gcgattcact ggtaacgagc
     4801 tcgggaaacc gggcctcgat ggcgttgacg cgctggcgca gcgcgtcata ggcggcgtcc
     4861 gtgacggttg gcgcgtcctc ctgataatag tgcttgtcgt ggctctcgat ttcgagcgcc
     4921 agccgtttca gctcgacctt ggcctgcgcc ttggtgaggg tgtcgacggc ggggggagtt
     4981 tttgctttgg attttgctat catggaggaa gttatagtcc gtcattccgg gatggtccca
     5041 acgggtcggc gctttgcgcc gcccgatgac aggctccgga ccagacccgg aatctcgaga
     5101 ttccgggttc gcgctgcgcg cgccccggaa tgacgatgtt gtgagcgatc gatgccatga
     5161 gctactacgt ctatatcctc gcgagcagaa aggatggagc aacctacctc ggtatcacga
     5221 acgatctcgt tcgcaggatt taccagcacc gaacaaaggc cgtccgcggc tttacttcca
     5281 agcataatat cacacggctc gtatggttcg aaatcctacg atgatcctct ctccgcgatt
     5341 tcccgtgaga aagaattaaa gaaatgggaa gcgaagttgg aagatcgaat tgatcgaagc
     5401 gcggaatcca aattgggatg acttgtatga gtcgatttgt agctgagatt ccgggttcgc
     5461 gctgcgcgcg ccccggaatg acgacaccgc ggatgacacc gttacattat taaccccgtc
     5521 attccgggat ggtccgaagg accagacctc agatgtgcaa ttgcacatcg gggaatctcg
     5581 agattccggg ttcgccgctt cgcgccgccc cggaatgacg ttaaaaatca cgccgtcgcc
     5641 gcgcgcagca gccgctcggc cgccgcccgc gcctcggcgg tgatctccgc acccgccagc
     5701 atccttgcga tttcctcgcg gcggtgatcg ggggcgagcg cgttgacgcg ggtggcgacg
     5761 cgcttgccct tgtcgagcga gtcttttgaa atcagcagat gatgatcggc gcgcgcggcg
     5821 acttgcgggg cgtgcgtgac cgccatcacc tggaccttgt cggcgagccg cgccagccgg
     5881 gcgccgatcg catcggccac ggcgccgccg acgccggtgt cgatttcgtc gaataccagg
     5941 gtgggcgcgg aaccgcgatc ggacagcacg accttgatcg ccagcaggaa tcgcgacagc
     6001 tcgccgccgg aggcgacttt catcaacggt cccggtcggg tgccgggatt ggtctggacc
     6061 cagaactcga tacggtcaaa accctgcggg cccggcgatt tgggatccga ttcgatttgc
     6121 gtcatgaatt ttgcgcggtc gagcttcagc ggcgcgagtt cggcattgac ggccttgttg
     6181 agtttgtcgg cggacttggc acgcgctgcg gatagcttgg ccgcggcact gttgtagcgc
     6241 ccgaccgctt ctgccgccgc cgcctccagc tttttcaact gatcggcgcc ggcgtcgatc
     6301 agcgccacat cggcggcgta tttcgcagcc agcgccgcaa gaccgtcgac cggcgtcgag
     6361 tatttgcgtg acgccgcgcg cagcgcaaac agccgctcct cgatgcgttc aagttcggac
     6421 ggatcgaaat ccgccgcgat cagcgccgcg gtgagatgct ggtcggcttc ctccagcgcg
     6481 ttgatggcgg catcgatcgc ctttaccgcg ggctcgacca gcgtgggcga atttccggcc
     6541 cggcgttcca gccggcgtac cgccgccgac agtgccgcga ccggcgaatg attgccgctc
     6601 acggcgtcct gggcctcgcg cagatcggcc gcgattttct cgccctgcat catcagggcg
     6661 cggcggccgg cgagcgtggt ctcctcgccg tccttgggcg cgagcgcctt caattcatcg
     6721 gcggcgtggc gcagatagtc cgcctcgcgg gcggcacgct ccatgccggc gcgatgcgcg
     6781 tcgagtatgg tgttcgcggc acgcctggcc tcccagagcg cttcgaccgc cgtgacgtcc
     6841 tcctcgaggc cggcaaacgc atcgagcagc cgccggtggg tcgaggcatc gaccagcgcg
     6901 cgctcgtcgt gctggccgtg aatctcgacc agcgtggagc cgaccgcctt cagggtctgc
     6961 acgctgatcg cctgatcatt gatgaaggcg cgggtacggc catcggcgag ctgcacccgg
     7021 cgcaggatca tttcgtgggt gtcatcggaa ctcgtatcgt cgagcccgtt gtcgctgagg
     7081 atcaccgagg ccggatggtc cctcgggaca tcgaacacgg cggtaacctg gccctgctcc
     7141 gcgccatggc ggacgaggcc cgaatcaccg cgaccgccga gcgccagcgc gaacgcatcg
     7201 agaaggatgg atttgccggc accggtctcg ccggtcaaca ccgcgaggcc gcgggaaaac
     7261 tcgatatcga gccgttcgat caggacgatg tcacggatcg aaagacgcgc cagcatgtga
     7321 gatcattcct agccgagacc cagtttcttg aaggccctgg agatgtagga accctgattc
     7381 tcgctcggct cgagacctcc ggactttaca agattatagg cgtccttgta ccagcggctg
     7441 tcgggaaaat tgtgcccgag cacggccgcg gcggtctgcg cctcgccgac aacgccgatc
     7501 gccatgtagg cctcggtcag ccgcgccagc gcttcttcca cgtgccgggt ggtctgatac
     7561 tgggtcacca cagtcttgaa gcggttgatc gcgccggtgt aattgcgctt ctcgagattg
     7621 tagcgcccga tatccatttc cttgccggcg agctgatcgc gcgcgccttc gagcttggcc
     7681 ttggcttggt tggcgtattc cgtggtcgga tatttgcgaa tcacctcttc gagcgcggca
     7741 atggccttct cggtccggcc ctggtcgcgg gaagtgtccg gaatctggtc gtaatgcgag
     7801 gcggcgatca ggtattgcgc ataggcggcg tcggggctgc cgggatgcaa ggtgacgtag
     7861 cgggtcgcag caccgatgca gctgtcgtag tcgcccgaat tgtaaaaggc ataggccgac
     7921 atcagaagcg atttgcgcgc ccagtcggaa tagggatgct gacggtcgac ttcctcgaat
     7981 ttcttcgatg cggcctttgc atccttcttt tgattcatca ggtacaagcc ctcattatag
     8041 agcttgtcgg cgggttcctc gacgaacgta tcgtccttgg ccaggaactt gtcccacagc
     8101 gcgccggtgc cgcagccgct cagggggacc gcgagaacaa ccagaccggc ggcgagccgc
     8161 agctgccggc cgatccggcg gaagccatcc ggtaccttgc gcagttcgag cgtcatacgc
     8221 tgtgccgaca tgaatctgag acctgatgcc tgtgatgcgg tgcccgccac gccatgaacg
     8281 cggtcacgga cgcgaaaacc catgcctgtg gcgcttgtcg tgcaaccgcg gggcctgttt
     8341 agctcataaa agtccgttaa ccaaccgaat acgcaggcat ttcgcccgga ttaaggcggg
     8401 taggattgga ttcgggccga tcatggttac cgcagcaaat tgagccgcaa atcgcctccg
     8461 gcgacgctca ggagacgttc aaaggaacgc cgaggagacc tgcagctgat tcccaggaca
     8521 tttcgaagac gaaagtccgg acatttcgaa gacgaaagtc cggacatgct caggacattt
     8581 cgcaagcgaa agtcaggaca cgtcggggcc gtaggccggg gcgatcatgc cgcccaccat
     8641 tccacttccg acttcggcgt gagtgcgcga acgacgcgcc gtttccgcct cgatcacccg
     8701 ccaggcgctg cgatcggcca gcagggcggt cagcacggcg tgattgagct tgtggccgcc
     8761 gcgtaccgag cgataggcgc caagcagcgg caggccggcc agcgccagat cgccgaccac
     8821 gtcgagcacc ttgtggcgtg cgcattcatc ggagtagcgc agaccctcga tgttgagcac
     8881 gcgggtctca tcgaacacca cggaattctc aaaggaggca ccgagcgaaa agcccgcact
     8941 ccagagccgc gcgacatcat tcatgcatcc gaaggtccgg gcgcggcaga tctcgcggcg
     9001 gaacctttca gggcttagat cgagcttgta gttctgacgg ccgatcaccg gattggtgaa
     9061 gtcaatttcg acctcggcac ggaaaccagc cgcatggggg cgcagctcgc cgaacgaggc
     9121 gcccatcgta acctgcaccg gcttcaaaac ctggatgaaa cggcgcgacg ccgacagggt
     9181 gacgataccg gcctgatcga tcgcggcgac gaaagtggcg gcgctgccat ccatgatcgg
     9241 aacttccgga ccatcgactt cgatcgtggc gttatcgacg cccatgcccc gcagtgcagc
     9301 aagcacgtgt tcagaggtcg agaccagcgg gccttcgcgg ttgcccagca ccgtggcaaa
     9361 ttcggttgaa atcaccgact cggcagaggc ctgaacctga cggtcgcggc cgtccagacc
     9421 ggtgcggaca aaaataaaac ctgcatccac gggtgcaggt ccaagagtca aacttgccgg
     9481 taaaccggaa tgaacgccga cacctgtcac ggtggcttgc gaccgaagcg ttgtctgccg
     9541 gctaaatttc atttcgaaag ctgcccacta ctgacttacc caaaagaccc gatccccccg
     9601 ttgggcgggc gaatcacgct tcaggtatcc ccaagtcgtg cgaaaccata gtcaccgccc
     9661 caaagcgcgc caactcacgc ttttttaccg attgttaccc tatctctgcc gtattcgccc
     9721 caaaattaac taaattaggc cacggttaac gcccagttcg gttttggcac cgcaacatca
     9781 gaatcgttat ttattgttta aaatcagttg cttgatcgac attgtcaggc attctcgcgg
     9841 ccataaacaa aggccccggt ttggagagcc gaggcctttt tggtcgtcag aattgtaaca
     9901 aacgtcagtt gttctgccgc cgcaggaagg cggggatatc aagatggtcg tcgccctgtg
     9961 gcgctgatgc aacaggcgcg gggcgtccat ggacgtccaa tccctgcggt gccggccgcc
    10021 tgccgtattc ggataccgga tcgccagccc cgatttgctg cgcgaccgcg cgctggggct
    10081 tgcgttccgc gtgcggcggt atcggggcca tggccggacc cgaggcgcgg gccgcgatcg
    10141 gcggttcggt ctcctcgtcg cgacggccga ggccgacatt ggcaaggcgc tgcagaagcg
    10201 acatccgggt cttctgcgga tggtcatcct cgacctcgcc gcgggcctgc cgaatttcgt
    10261 tctgcgcagg caccggcagc tcttcgaatt tcggcatcct cggcgcgcgg accggggcac
    10321 gttcggccgc ctgcgggatg aatgtctcgg acgtcggggg ctggtgggcc tcgacatgcc
    10381 cggtaccatg atccggaaac aaggtcggct tctgtgcaat cgcgatcgga cgaaccgtga
    10441 catcgccgta ggacgcgggc tgtagcggtg cggatgcagc cagctgcgcc tgctcgggag
    10501 cgacagccgc ggcgatggcc gccagcgccg cgcgttcgat gccgcttgag gcgcgctgct
    10561 gcagggcagc ggaaggaacc tgcgctgctg cggatggatc aagtttctgg gcgcgttcgg
    10621 cgagacgctg attgtcggcg cgcagccgtg ccgtgagatc ggccaggcgg ttgtccggcg
    10681 tcccgctcga agccacggcg ctggccgggg cagccgcggt cgggtttgcg ttgcgcgaga
    10741 tcagcgcctg ctcgatgccg gtggcaacga cggaaacgcg aatcacgcca tcgaggttct
    10801 cgtcgaaggt ggcgccgacg atgatattgg cgtcctgatc gacctcctcg cgaatccggg
    10861 ttgcggcttc gtcgacctcg aacagggtca gatcccggcc gccggtgatc gagatcaaga
    10921 ggccgcgggc gcccttcatc gacgaatcgt caatcaacgg attggcgatc gcggcttcgg
    10981 ccgccgtcag cgcgcgcttt tcgccggagg cctcgccagt gcccatcatc gccttgccca
    11041 tctcgcgcat cacggcgcgg acgtcggcga agtcgagatt gatcaggcct tccttgacca
    11101 tcagatcggt gatgcaggcg acaccggaat acagcacctg gtcggccatc gcgaaggcgt
    11161 cggcgaaggt ggtcttttcg ttggcgaccc ggaacaggtt ctggttcggg atgatcaaca
    11221 gcgtatcgac caccttgtgc agttcggcga tgccggcctc ggcggtgcgc atgcggcgct
    11281 ggccttcgaa gtggaacggt tttgtcacca cgccgaccgt gaggatgccc atgtcgcggg
    11341 cggtcttggc gatgaccggc gctgcgcctg tgccggtgcc gccacccatg ccggcggtga
    11401 cgaacaccat gttggcgccg gagagatggt cgcggagctc gtcgatgacc tcctgggccg
    11461 cggccgcgcc gacatccggc tgcgaaccgg cgccgaggcc ctgcgtgacc tgggtgccca
    11521 tctgcacgat gcgctgggcc ttggacatgg tgagcgcctg cgcgtcggta ttagcgacga
    11581 cgaaatcgac accctgcagt ccggcggtga tcatgttgtt gacggcattg ccgccagctc
    11641 caccgactcc gaatacggta atccgcggct tcaactcgct gatgtcaggg ggggtaagat
    11701 tgagtgccat ggttgcctct cgattacgac gcgcgcgttg gttcgcctcg gccgacggcc
    11761 gcgggagctt ggggaagtcg aatgggtcgg aaaagattca tcagaagccc tcgcgaagcc
    11821 atcgtccgac ccttccgaaa tagccgtcag ttcctgtcct gagctgccgc gtatgccgcg
    11881 gttcgacgtg ttcaagatgc gcgtattgcg gataaaccag cagacccgag ggaaccgcga
    11941 acgacgcgtt cttcgcctcg ctcggcagcc ggccaaagcc gagtggacgg ccgatgcgca
    12001 ccggccggcc gagaatctgg gtcgcaagtt cgagggtgcc ggagagctgc gaagcgccgc
    12061 cgctcaagac cacccgcgcc ttgggttctg ccgcaaaggg cgaatccgcg agccggtccc
    12121 tgaccatttc aaaaatctcc tcggcgcgtt gtcgaacaat gttcgcgatc gtggctcgag
    12181 atacgatctg cggcgcatcc cgctcgttgt caccggcagc cggcacagac atcaactcac
    12241 gcgcgtcgga cccgccggtc agcacggtgc catataacgt cttgattcgc tcggcatccg
    12301 caatgcatgc gctcaagccc cgtgcaagat ccatggtgat gtgctggccg ccgaccgcaa
    12361 atccgctggc atggacgaac cgaccgccgg cataggtcgc gatcgtggtg gttccagcgc
    12421 ccatttccac cacggcggcg ccgatatcgg cctcatcgtc ggtcagcacc gacaggccgg
    12481 ccacataggg gctcgccgcc atggcttcga cattgagatg acaacgttcc accaccagca
    12541 tgaggttttt ggcgaccgtg gcctcgaccg taacgacgtt catgtcgacg ccgaattgcc
    12601 gggccaccat gccgcgcggg tcccgaatgc ctttgacgcc gtccagcgcg tagccgaccg
    12661 gcagcgcgtg cagcacggta cggccctccc cggtggcgtg gcgcatcccg gtagaggtga
    12721 cgcggatgac gtcggctgac gtgaccgcgc cgccacgtat atccgccgcg gcttcgacca
    12781 attgaccgtg cagccggcca cccgataccg agagcagcac ggactcgacc cggaccttgg
    12841 ccatgcgctc ggccagcgcg accgcatgac gcaccgcctg ctcgcattcg gcgaggtcga
    12901 ccaccgcgcc ggccttgaca ccgcgcgact ggatctggct gtagccgatc aactcgacgg
    12961 cgtgggtacg gccgcgcaag gcgtcgctcg gcgggcacgg ccggagccgc gcgatcatgc
    13021 aggcgatctt gctggtgccg atatcgagcg acgccaccag cgcggtgcgc ttctgcggca
    13081 tcgggcgcgt cttcggcgtt tgattgcgat caaggccggt catgcgtcac cggccttttt
    13141 cttcggcttc ttgtccttga acagatcttc gcgggccttg gccgcgtctt ccgataattg
    13201 cacggtcagc cggtccggca gacgcatgtc gacggcgacg atatcgcgtg agaacaaccg
    13261 gtcttccttg tcgagcttgc tcagcgtcgc cagcgcgttg ccgacatctt tctccggcag
    13321 ccggatatcc agaccgtcct tgagccgcag gttccagcgc cgttcgccca ccaaaatcgc
    13381 ggcctttgtc gcagcattga cctgcgggta gcgcgcgagc agcgcgagga aatccctcgc
    13441 gcgggtatcg gcgcccttgc cgacgaccag cggcagcgag acaaaacgct gcgacatgta
    13501 gcgctcaagc acagcgccat catctgaaat caccgacagc cggccatcct gctgccataa
    13561 cgcgaatgcc gaacgctcga cgatatcgat ctggagttga ccgggataaa gcttcagcac
    13621 cgtcgcttcg ccgatccacg gattggcctt cagcttctcg cgcacggccg cggcgtcgag
    13681 gaacagcagc gaggagcggc cgttgacgcc gccgatcgcg aggacctcgt cctgggtcaa
    13741 ttgcttgcgg ccgttgatgc cgaccgccgt gatccgaaat cccgcggaat ttgcgatcgc
    13801 attgcgggtg tcgctcagcg ccgaggataa ttcctcgaca tgaccgcccc tcacgatgcc
    13861 gaagccggcg ctgccaagca gcatcaggac ggtcgcggcg acgccggcgc ggtgcggaat
    13921 gtaacgctcg agcagcgcga tcaggcggtg cggatgctgt tcgcgttccg ggcgcgccct
    13981 ggcgccggca cgatgatagc ccagccgctc gcgcaacagc acggccgcgc caatggcggc
    14041 cgccttcagg tcagtttggg gcctcggcga tcccaacgat cgggcgaggc gtcctccacc
    14101 atccattgca caagctcgtc aaatgtgtgc ccgcaaatat tgcgagttct ggcacaagtg
    14161 acgtcctggt cgtgccgggc tgggtctcga cctcgatgca aaagaggcca cctgttccct
    14221 cgatgcgggc gtcgacacgg aaatccgcac ggctccacgc cccggcagcc aagcgccaaa
    14281 atgcgccgcc tgcgttaacc ttcggacttc ctggtaaaca aatggtaaat taggtcgagg
    14341 gtggccgtga tttgatccgc cttttgaata ctttgccccc agaatcctgg aactttaccg
    14401 aaagttaagg atttcgatca ccccggccgg actgccaatg catttcccgg cttcttgcta
    14461 tctatgcgtg tgctctttcg ccaatgcgat ctggccctgc atgaaatcgg cgaactcgat
    14521 tccttgagcc ttgagcgact tcggatagag cgaagccttg gtcaggcccg gcaaggtgtt
    14581 ggtttcgaga aaaatcggtc ccttctccga gatgatgaag tcggtgcggg aatagccgct
    14641 gcacgacatc gcccggtgcg ccttgaccgc ctggtccatg atttgcgcgg tgatttcagg
    14701 tgcgaaccgc cccgggcaaa tctcctgggt cgatttgagc agatatttag cggtgtagtc
    14761 gaacgcgcct tcccccggga cgatttcgat cggcggcagc gccacgagcg agccgtccgg
    14821 ttgctccagc acgccgcagg tcgcttccac gcctgcgatg aatggttcaa tcagatattc
    14881 ttcggtcttg gccgcgttgc ggacggcgac gaggtcctgc ttggcattga cgaagatcag
    14941 gccatagctc gatccatccc gtaccggctt ggcaatcagt cggccgtatt cggccagcgc
    15001 ggcatcgata tcgtccagca tcaggccgat cggcgccgtc acgccggcga tagcggcaaa
    15061 gcgcttggcc gcgaccttgt cgaaggcgag atgcgaggac gccgagcctg agccggtaaa
    15121 gggaatcccg cgcatctcgc acatcgcctg aagctcgcca ttctccgccc tgcccccgtg
    15181 cagcccgagc accagcaagc gctcctcggc tattgcctta tcaagcgccg gttccagtcc
    15241 gatgccgcga ttgccgggtt tgaaagcgtc ctcgaacggc ctcgcgtgac tgcgaagcgc
    15301 ctgaggctga acctcatgca ccgtatcact gacgtcccag aaccacagat ccgcctccgg
    15361 cagagccgcg tgcatcgcct gggcggacgc taccgacacg agacgttccg tgttggtacc
    15421 gccgaaaaga atggtaaccc gcatcaggtt cgctcgatcc caatccgctt gatttcccag
    15481 tgtagctcaa ttcccgaatt ctctttcacc ctggcacgaa cggtctcgcc cagggtctcg
    15541 atatcatggc cggtcgcggc gccggtattg atcaggaagt tgcagtgcat ttccgagacc
    15601 tccgcgtcgc cgacccgcag gcctcggcag ccggcggcat cgatcagctt ccaggcgctt
    15661 tggcccggcg gattcttgaa ggtcgagccg ccggtctttt cgcgaatcgg ctgagcggtt
    15721 tcgcggtggt tctgcacctc gttcatgcgg acgcggatcg cctccggcgc ggccatcagc
    15781 ccgcgaaagc gggcggccgt aaagatgatc gagggatcga cgccgctgtt gcgatagatg
    15841 aacttcatgc cggcattgtc gaatatttgt tttctgccgt cgcggccaat accgcgagcc
    15901 tcgaccaaaa catccttggt ttcagcgcca ttggcgccgg cgttcatgcg cagcgcgccg
    15961 ccgatggtgc cgggaatgcc gaagaagaat tccagcccgc cgatattggc cgctaccgct
    16021 gcctcggcga cgcgcttgtc gagcgcagcc gcaccggcgc tgacgatatc gcgattggcg
    16081 ctggtctcgc cgaaaccacg cgggccaagc cggatcacga cgcccggcat gccaccgtcg
    16141 cgcacgatca ggttggagcc aacgccgacg acgtagaccg gaatctcact cggcagatgc
    16201 ttgaggaaat aggcgaggtc atcttcatcc gatggcgtga acagcagctg cgccggaccg
    16261 ccggcccgaa accacgtcag ctcggccagc gactggttcg ccagcagccg tccgcgcagt
    16321 tgcggcatcg cggccttgag atcgggcgtg atgtcgggga aggtcatgcg tacttgtcac
    16381 agcaattagg ctggttcaat tcgagcggtt cgacgacatc cgtgacgcca tccagcgcga
    16441 gcacaatatc aagcattggc cccgcgcatg gaaggtccgg aagatcgccg ccatgaatcc
    16501 agattgggac gacctgttcg acacgatcgc gaagtaaaga cgtggatggc cgggacaagc
    16561 ccagccatga cgaatttgga cgctgtgcca actgctgcga acacatcacc ccagcgcctt
    16621 caattcgccc ggcagcgcgt aggcccattg cgtgatgttg ccggcgccga gacagaccac
    16681 aaaatcgccg ctgccggcga ggccgtgcac cacacgcgca aggtcggcgg atttctgcag
    16741 cgcaatcacc tcgcgatggc cgtgcgcgcg caagcccagc acaaaattgt cgcggtcgat
    16801 cccggcaatc ggcgcttcgc cggccggata aacctccgcg acgacaaccg tatcggcgtc
    16861 attgaaacag gtacaaaatt cctcgaacag cgactgcagc cgagtgaagc gatgcggctg
    16921 caccacggcg atgatcttgc cgttggtcga ttcccgtgcc gccttcagca ccgctgatat
    16981 ctcgacggga tgatggccgt aatcgtcgat cacggtgacg ccgttccact cgccggtcct
    17041 ggtgaagcgc cgcttgacgc cgccgaagcc cgcgatcgcc ttgcggatcg catcgtcgga
    17101 aagtccgagc tcgtgcgcca ccgcgatcgc cgcggtggcg ttgagcgcgt tgtgccgtcc
    17161 cgccatcggc agcatgagac cagcgatctc atgcgccata ccggtcttgc gatcgcggaa
    17221 cacgaccttg aagctggaac cggcgcccat cggtaccaga tcgaccaggc gcgcgtcggc
    17281 ctgcgggttt tcgccatagg tgacgatgcg gcggtcctcg atattgccga cgatgctctg
    17341 caccaccgga tggtcgatgc acatcacggc aaaaccgtag aacggcacgt tctcgacgaa
    17401 attgcggaac gcttcctgca ccgcctcaaa agtcttgaaa tgatcgagat gttcggggtc
    17461 gacattggtg acgatggcga catcggccgg cagtttcagg aacgtgccgt cgctctcgtc
    17521 ggcttccacc accatccagt cgccggcgcc gagccgggcg ttggtgccgt aggcattgat
    17581 gatgccgcca ttgatcacgg tcggatcgag gttgccggcg tcgagtagcg ctgcgaccat
    17641 cgaggtcgtg gtggtcttgc catgggtgcc cgagatggcg acgcagcttt tcagccgcat
    17701 cagttcggcc agcatttcgg cacggcgcac cacgggaata cgctgggcgc gcgccgcgat
    17761 caattcggga ttgtcgcgct tgatcgcggt cgacaccaca agcacatcgg cgccgtcgac
    17821 attctcggcc gtgtggccga cggtgatggc aatgcccttg tcgcgcagcc ggttgacgtt
    17881 ggcgctctcg gaagcgtccg acccctgcac ggtgtagccg agattgctta agacctc
//
LOCUS       DATNOI010000561         7537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_420984,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000561 DATNOI010000000
VERSION     DATNOI010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420984"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VN968_22635"
     CDS             complement(<1..338)
                     /locus_tag="VN968_22635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32064.1"
                     /translation="MNNKLLSCLVAGALSATLALAPPAFARGGGGGGGHGGGMGGGMH
                     AGGMGGGMHGGGMGGGMHGGGMGGGMHFAAMGGGGAHFAGSHFAGPHFAHVGRFGFRD
                     RDHFHHLFHR"
     gene            complement(639..1415)
                     /locus_tag="VN968_22640"
     CDS             complement(639..1415)
                     /locus_tag="VN968_22640"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXL32065.1"
                     /translation="MARTPVLAAGGIVLRREKTPLIAVVRLRKRDEWVLPKGKLDDGE
                     TPRDAAEREVLEETGHDVSVHEFLGTLVYESGGRSKVVHYWRMETNGGQAYDLMDDVR
                     AVDWLPLDAAVERLSRTCERAFLANVGPLALEAAMLARVPRRPKAKPRVPAKPRDRRA
                     VAPRPSLTIPVPVPAPPQPDLTPVATLRDDHQSVVSDVVETKAPVGPTESETAPQIVE
                     ADAAMPAVEPEPSNDDTLEAAQGRRRNLVQKMRDWLRRAA"
     gene            complement(1517..2395)
                     /gene="asd"
                     /locus_tag="VN968_22645"
     CDS             complement(1517..2395)
                     /gene="asd"
                     /locus_tag="VN968_22645"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046080.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HXL32066.1"
                     /translation="MTIRGLISALTQQEDLNFLLTNRIPRAALTRFMGWFGKIEQPLV
                     RDLSIACWRLFSDLDLSEARKTDFKSLHDCFTRELKPGLRPPDPDPSIVVSPSDAIIG
                     AFGAIADDELFQIKGAPYSLLDLLGDPALVEAHRHGRFITLRLTSSMYHRFHAPCDCR
                     IEQVTFIHGDTWNVNPIALKRIEKLFCKNERAVIRTRLESGDALTLVPVAAILVASIR
                     LHFLDIVLNAESRGPTVFPCDVGVRKGDELGWFEHGSTIIVLAPDQFEFCDNVVESAR
                     ILAGQPLMRRSLARSV"
     gene            2430..4094
                     /locus_tag="VN968_22650"
     CDS             2430..4094
                     /locus_tag="VN968_22650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXL32067.1"
                     /translation="MDSPLPILSLAAAATAVAAAGFPKAQARLALSRAKHRSLGGHSK
                     MSRMVARLIPFYEFDINDFFCSDGAPPAVATQRQDGFFRLAHLYQDKFARGRQMTALA
                     SEKISDLQFTEAYRVPFQYSRLVREHLGTGAFVQSSAGVTVTDLDGNLFYDLTGSYGV
                     NIFGNDFYKECIASAEGRAHALGPVLGPYHPVIVDNVKRLCAISGLDEVSFHMSGTEA
                     VMQAVRLARYHTRRSHLVRFAGAYHGWWGDVQPGVGNPISPHETYTLADMSERTLRVL
                     RTRRDIACVLVNPLQGLHPNGNAPADSALVDSSRTGHFDRAAYSAWLQSLRQVCTERN
                     IVLIFDEVFVGFRLAAGGAQEYFGVRADMVTYGKSLAGGLPVGAVCGRKDLMRRFRED
                     RPVDVCFARGTFNSHPYVMTAMDEFLNRLDDPSFRSVYDGLDDTWNKRARRLNDKLTE
                     RDLPVRVCNLSSIWMVHYTEPSRYNWMFQYYLRLEQLALSWVGTGRLIFSLNYTDADF
                     TEVAKRFVTAAEKMKQDGWWWRDASLTNKSIRRQILKEMFTRRRQR"
     gene            complement(4122..4751)
                     /locus_tag="VN968_22655"
     CDS             complement(4122..4751)
                     /locus_tag="VN968_22655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32068.1"
                     /translation="MIKNFREQLRTQRWDDHRYYHHSRINQSLHFVSAFSFLVGYALL
                     FVDPVAAALVGWLVSMTTRQAGHFFFEPKGYDHINQATHDHKEEIKVGYNLNRKIVLM
                     TIWALSPVVLFFDPTLFGMFTPWGSPADFMRQVAKVWLAIGIGGLLFRTVHLFFIRDV
                     ETGLVWMTKILTDPFSDLKLYYKAPLFLLRGELIDPGLDLIEQGVEKHA"
     gene            complement(4875..5564)
                     /locus_tag="VN968_22660"
     CDS             complement(4875..5564)
                     /locus_tag="VN968_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXL32069.1"
                     /translation="MAEIIKLDGTTQLDFDREMVEQAYDRWAPIYDLVFGGVFSKGRK
                     AAIQTTNRIGGRVLEVGVGTGISLPQYAPHLRIFGTDISEAMLQKAKKRVAELRLKNV
                     EGLAVMDAEKLEFPDNSFDVVMAQYVVTAVPNPEVALDEFARVLRPGGELIILTRVSA
                     DAGVRRFIEQRLQPVVRPLGFRTAEFAWSRYARWLAGARGMELVERRLMPPLGHFSLI
                     RFRKTGVAAAA"
     gene            complement(5618..6673)
                     /locus_tag="VN968_22665"
     CDS             complement(5618..6673)
                     /locus_tag="VN968_22665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXL32070.1"
                     /translation="MRILIATDAWHPQVNGVVRTLTSLARSALAFGAEINFLTPDGFA
                     SLGVPTYPGLRVALPNRREIARRIEAAAPDAIHIATEGPIGWAVRAYCRRRRLAFTTS
                     YTTRFPEYIAARSIIPAGVSYAVLRHFHAAAAMTMVATASLKQELGARGFANLGTWTR
                     GVDTGLFSPDEPAELDLPRPVFMTVGRVAVEKNLEAFLSLSLAGSKVVIGDGPQRALL
                     ERRYPKVRFLGEKTGTDLTSHLAAADVFVFPSRTDTFGVVQLEALACGTPVAAFPVTG
                     PLDVIAGHAVGALDTDLRSACIRALGMSRETCRNFALERSWENSARQFIGNLSALQPS
                     RSLQPARRVAASGAVRS"
     gene            6807..>7537
                     /locus_tag="VN968_22670"
     CDS             6807..>7537
                     /locus_tag="VN968_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXL32071.1"
                     /translation="MTTSDLNEPPGVSARRRKRQNAALLVLAAGVFLFAAAAGGLYYA
                     LRPVTLRIAVGPAGSDDQKLIQALAHTFSRDASSVRLSPIATEGATESIALLAAGKAD
                     LAVARGDLDLPANAESVAILRKNVVVLWAPSGLPPKGSKKKPAPKIKGLDDLAGHHVG
                     VIGRTQANVTLLRVILTESGVNSEKVAITQFGTNQIAEMTRDPTLDAFMAVGPLDSKI
                     TIDAIAATAAAKGEPKFLPVDVSEA"
BASE COUNT         1459 a         2380 c         2313 g         1385 t
ORIGIN      
        1 aagcgatgga agaggtgatg gaagtggtcc cggtcccgga acccgaacct tccaacatgc
       61 gcaaagtgcg ggcccgcaaa atggctgccg gcaaaatgcg cgccgccgcc gcccatggcg
      121 gcgaaatgca tcccgccacc catgccgccg ccatgcatcc cgccacccat gccgccgccg
      181 tgcatcccgc ctcccatgcc gccggcatgc atgccgcctc ccatgccgcc gccatggcca
      241 ccgccgccgc caccgccgcg cgcgaatgcc ggtggcgcca gagcaagcgt tgcactgagc
      301 gcacccgcga ccaggcaact caagagcttg ttattcatcc caatcctccg tggttgaatt
      361 ccttcgacgg acgccgtcgc aagaagacca ccagcaaacc gaaaggatcc aatgatttca
      421 aatgacaagt gtgacagttt ctgacacgga agttagtttc atcgactcgc gggaagcgaa
      481 aggcggccct gcggagcgcg tcagagtaga aaggcttttt tcggagacga aagccttttc
      541 agagcagcaa gtcttttcag agcggcaagc cttttcagag cagcaagcct tttcagacca
      601 gcaagccttg cacgaccagc aagccttgca cgaccaggtc aggctgcgcg ccgcagccag
      661 tcccgcatct tctgaaccag gtttctgcgc cggccttgag ccgcttccaa cgtatcgtcg
      721 ttactcggtt cgggctccac tgccggcatc gcggcgtctg cttccacgat ctgcggcgcc
      781 gtctcggact ccgtcggacc gaccggtgct ttggtttcca ccacatcgct cacgacagat
      841 tgatggtcat cccgcagggt cgccacgggc gtcaaatcag gctgcggcgg agccggcacg
      901 gggactggta tcgtcaacga ggggcgcggc gcgacagccc gccggtcgcg aggctttgcg
      961 ggaacgcgcg gctttgcctt gggccgccgc gggacgcggg cgagcatcgc ggcctcaagc
     1021 gcgagcggac cgacattggc caggaacgca cgctcacagg tgcgggaaag ccgctcgacg
     1081 gcggcgtcga gcggcagcca gtcgaccgcc ctcacatcgt ccatcaggtc gtaagcctgc
     1141 ccgccattgg tttccattcg ccagtagtga acgactttgg accgtccgcc ggactcatag
     1201 accagcgttc caagaaactc gtgcaccgaa acatcatgcc cggtttcttc cagcacctcg
     1261 cgttcggcgg cgtcgcgtgg cgtctcgccg tcgtcgagct tgcccttggg cagcacccat
     1321 tcgtcgcgct tgcgcaagcg caccaccgcg atcagcggag ttttctcccg ccgcagcaca
     1381 atgcctcccg ccgccaatac gggcgtccgc gccatctcac gtcccatgtt tgcgatttcg
     1441 aatcgtttcg accgctacca tataatgtcg tgcggcggta aaatcgagat tggcatgggc
     1501 ccgcgtgccg aagcggctag accgaccgcg ccagcgatct tcgcatcagg ggttgaccgg
     1561 ccaaaatgcg cgcggattcc accacgttgt cgcaaaactc gaattgatcc ggcgccagca
     1621 cgataatcgt cgatccgtgc tcgaaccagc cgagttcgtc gcccttgcgg acgccgacat
     1681 cgcagggaaa aaccgtcggt ccccggcttt ccgcattgag cacgatatcc agaaaatgca
     1741 gccggatgct ggcaaccagg atggccgcga ccggcaccag ggtcagtgcg tcgccgcttt
     1801 ccagccgggt gcggataacc gcgcgttcgt tcttgcagaa cagcttttct atacgtttca
     1861 gcgcgatcgg attgacgttc caggtatcgc catggatgaa tgtgacctgc tcgatccggc
     1921 aatcgcaggg tgcatgaaag cggtgataca tgctggacgt cagccgcagc gtgatgaacc
     1981 gcccatgtcg atgcgcttcc accagcgcag gatcgccgag cagatcgagc agcgaatacg
     2041 gcgcgccctt gatctgaaac agttcatcgt cggcgatggc gccgaacgcg ccgatgatgg
     2101 cgtcgctcgg actgaccacg atcgacgggt cgggatcggg cggacgcaag ccgggcttga
     2161 gctcgcgcgt aaaacaatca tgcaagctct tgaagtcggt cttgcgcgcc tcggaaaggt
     2221 cgagatcgga aaacagccgc caacaggcga tcgagagatc gcggaccagg ggttgctcga
     2281 tcttgccgaa ccagcccatg aaccgggtca gggcggcccg cgggatacga ttggtcagca
     2341 gaaagttcag gtcttcctgt tgggtcagag cggagatcag gcctcgaatt gtcatgaatc
     2401 tgtcagctgg attgattagc gctggtggca tggattcgcc gcttccgatt ctctctctcg
     2461 ccgccgcagc gaccgccgtc gccgcggcag gtttccccaa agctcaggcc cgactggcgc
     2521 tgtcgcgagc caagcaccgc tcgctcggcg ggcactcgaa gatgtcgcgc atggtcgcgc
     2581 ggctgatccc gttctatgaa ttcgacatca acgatttttt ctgttccgac ggtgcgccgc
     2641 ccgcggtcgc gacccagcga caggatggct ttttccggct cgcgcacctc tatcaggaca
     2701 agttcgcccg cggccggcag atgacggcgc tggcctcgga aaagatctcc gacctgcaat
     2761 tcaccgaggc ctaccgggta ccgtttcagt acagccggct ggtccgggag catctgggca
     2821 ccggcgcctt cgtgcagtcg tcggcggggg tcacggtgac cgatctcgac ggcaacctgt
     2881 tctacgatct gaccggctcc tacggcgtca atattttcgg caacgacttc tacaaggaat
     2941 gcatcgccag cgccgagggg cgcgcccatg cgctcggccc ggtgctcggg ccctatcacc
     3001 cggtcattgt cgacaatgtc aaaaggttgt gcgcgatttc ggggctcgac gaggtctcgt
     3061 tccacatgtc cggcaccgaa gcggtgatgc aggctgtgag gctcgcccgc tatcacaccc
     3121 gccgctcgca tctggtgcgt ttcgccgggg cctatcacgg ctggtggggc gacgtgcagc
     3181 ccggcgtcgg caacccgatt tcgccgcacg aaacctatac gctggccgat atgtcggaac
     3241 ggacgctccg ggtgctgaga acgcggcgcg atatcgcctg cgtgctggtc aatccgctgc
     3301 aggggctgca ccccaacggc aatgcgccgg ccgattccgc gctggtcgac agttcgcgga
     3361 ccggccattt cgatcgcgcc gcctacagcg cttggctgca gtcgcttcgg caagtctgca
     3421 ccgagcgcaa catcgtcctg atcttcgacg aagtctttgt cggcttccgg ctcgccgccg
     3481 gcggcgccca ggaatatttt ggcgtgcgcg ccgacatggt gacctatggc aaaagcctcg
     3541 cgggcggact tccggtcggc gcggtgtgcg gacgcaagga tctgatgcgc cgctttcgcg
     3601 aggatcgtcc ggtcgatgtc tgcttcgcgc gcggcacctt caattcgcat ccctatgtca
     3661 tgaccgcgat ggatgaattc ctgaaccggc tcgacgatcc ctcgtttcgt tcggtctatg
     3721 acgggctcga tgacacctgg aacaagcggg cgcggcggtt gaacgacaag ctgaccgagc
     3781 gcgacctgcc ggtgcgggtc tgcaacctgt cgtcgatctg gatggtccat tacaccgagc
     3841 cgtcccgcta caattggatg tttcagtatt atctgcgcct cgagcaactc gcgctgagct
     3901 gggtcggaac cggccgcctg attttcagcc tgaactacac cgacgcggat tttacggaag
     3961 tggcgaaacg ctttgtcacg gcggccgaga agatgaagca ggacggctgg tggtggcgcg
     4021 acgcctcgct caccaacaag agcatccgcc ggcagatcct gaaggagatg ttcacgcgaa
     4081 gacggcagcg ctaaaacgcc gccgtcctcg ttggtcgttg ttcaggcgtg tttctcgaca
     4141 ccctgctcga tcagatcgag gcccggatcg atcagctcgc cgcgcagcag gaataagggc
     4201 gccttgtagt aaagcttgag atcgctgaac gggtcggtca ggatcttcgt catccacacg
     4261 aggccggtct cgacatcgcg gatgaagaac agatgcacgg tgcgaaacag caggccgccg
     4321 atcccgatcg ccagccagac cttggcaacc tggcgcatga aatccgccgg tgaaccccag
     4381 ggcgtaaaca ttccgaacag ggtcggatcg aagaacagca ccaccggcga gagcgcccag
     4441 atcgtcatca ggacgatctt gcggttcaga ttgtagccga ccttgatctc ttccttgtga
     4501 tcgtgggtcg cctggttgat gtgatcgtag cctttgggct cgaagaagaa atggcccgcc
     4561 tgccgggtgg tcatcgagac cagccagccc accagggcgg cggccaccgg atcgacaaac
     4621 aacagcgcat agccgaccag aaagctgaag gcgctgacga aatgaaggct ctggttgatg
     4681 cggctgtgat gatagtagcg atggtcatcc caccgctggg ttcgcaattg ttcccgaaag
     4741 ttcttaatca tcttctcccc caaaggtagg ttgggattcg tttactgaga ttcgatgtcc
     4801 actgtgtgac aacatcataa tgacatatgg cgttttcaca acgtcatggt cggccaacgc
     4861 ttgctcgcgc gagcttaggc ggcggctgcg accccggtct tgcggaaacg gatcagcgaa
     4921 aagtggccga gcggcggcat cagacggcgc tccaccaatt ccattccgcg cgcgcccgcc
     4981 agccatctgg cgtaacgcga ccaggcgaat tcggcggtcc ggaagccgag cggacgcacg
     5041 accggctgca gccgctgctc gatgaaacgg cgcacgccgg catcggcgct gacccgggtg
     5101 aggatgatca gttcaccgcc cgggcgcagc acgcgggcaa actcatccag cgcgacctcc
     5161 gggttcggca ccgcggtcac gacatattgc gccatcacga cgtcgaacga attgtcggga
     5221 aattcgagct tctcggcgtc catcaccgca aggccctcga cattcttcag ccgaagctcg
     5281 gcaacgcgct tcttggcctt ttgcagcatc gcctccgaaa tatcggtgcc gaagatgcga
     5341 aggtgcggcg catattgcgg caacgagatg ccggtgccga cgccgacctc caggacgcgg
     5401 ccgccgatcc tgttggtggt ctgaatagcg gccttgcggc ctttgctgaa cacgccgccg
     5461 aacacgaggt cgtagatcgg agcccagcga tcataggcct gctcgaccat ctcgcggtcg
     5521 aagtccagct gcgttgtacc gtcaagtttg atgatttctg ccatggggaa actccaaccc
     5581 tttgttgcag agtctgaaat cagactcggt gttgatctca acttcgcacg gcgccgctcg
     5641 ccgccacgcg gcgggcgggt tgcagcgagc ggctcggctg caatgcgctc agattgccga
     5701 tgaactgcct ggcgctgttt tcccaggagc gctcaagggc aaaattgcgg caagtttcgc
     5761 gcgacatgcc gagcgcgcgg atgcaggcgc ttcgcaagtc cgtatccagg gcgccaaccg
     5821 cgtgaccggc aatcacatcg agcgggcccg tgacgggaaa tgcggcgacc ggcgtgccgc
     5881 aggccagcgc ttcgagttga acgacgccga aggtgtcggt gcggctcggg aagacaaaga
     5941 cgtccgcggc ggccagatgg gaggtcagat ccgtgccggt tttttcgccc aaaaatctca
     6001 ccttcgggta gcgacgctcc aacagcgccc gctgcggccc atcgccaatc acaaccttgg
     6061 atccagccag gctcaaggag agaaacgcct cgaggttttt ttcgaccgcg acacggccga
     6121 cagtcatgaa gacaggtctg ggcaaatcga gctcggccgg ctcgtcgggg ctaaagaggc
     6181 cggtgtcgac gcctcgcgtc caggtgccga gatttgcaaa acctcttgca ccgagttcct
     6241 gcttgagcga ggcggtcgcg accatcgtca tggcggcggc cgcatgaaag tggcgcagca
     6301 cggcatagct cacgccagcc ggaatgatcg agcgtgccgc aatgtattcc gggaagcggg
     6361 tcgtatagga agtggtgaaa gcgagcctgc ggcggcggca ataggctcgc accgcccacc
     6421 cgatcggccc ctcggtcgcg atatgaatgg catccggcgc ggccgcctct attcggcggg
     6481 caatctcgcg ccggttcggc aaggccaccc gcagccccgg ataggtcggg acacccagcg
     6541 aggcaaaccc gtccggggtc agaaagttga tttcggcgcc aaacgccaaa gcgctgcggg
     6601 ccaacgaggt taacgtgcga accacgccgt tgacctgcgg atgccacgcg tcggtcgcaa
     6661 ttaggatgcg catcatggga acctgagagc tatgattctc aagtccgacg ttccgctaag
     6721 gatatttcaa gcgtgtgacg tcatcgatgt gtagtcatgc ttttttgggg gtcgcctcga
     6781 actatttcgt gtagaatggc agcacaatga cgacatcaga cctcaacgaa ccgccgggcg
     6841 tgtctgccag gcgccgaaaa aggcagaacg ctgcgctgct ggtgctggcc gccggcgtgt
     6901 tcctttttgc cgcggccgcg ggcggattgt attatgcttt acggcccgtc accttgcgga
     6961 ttgccgtcgg cccggcggga agcgacgacc agaagctcat tcaggcgctg gcgcacacct
     7021 tttcccgcga tgcgagttcg gttcggctgt cgccgatcgc aactgaggga gccactgaaa
     7081 gtattgcgct gcttgcggcc gggaaagccg atcttgccgt cgcgcgcggc gacctggact
     7141 tgccggccaa cgccgaatcc gtcgccatcc tgcgcaagaa cgtcgtcgtg ctgtgggccc
     7201 cttccggcct gccgccgaaa ggttcaaaaa aaaagccagc accaaaaatc aagggtctcg
     7261 acgatctcgc gggacatcac gtcggcgtca tcggtcgaac ccaggcgaat gtgacgttgc
     7321 tgcgcgtgat cctgaccgaa tcgggcgtca attccgaaaa ggtggcgatc acccagttcg
     7381 gcaccaatca gatcgccgag atgacccgcg atccgaccct cgatgcgttc atggccgtgg
     7441 gcccgctgga cagcaagatc accatcgacg ccattgccgc gaccgcggct gccaagggtg
     7501 aacccaaatt tctcccggtc gatgtgtcgg aggcgat
//
LOCUS       DATNOI010000562        13518 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1787764,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000562 DATNOI010000000
VERSION     DATNOI010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13518
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787764"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VN968_22675"
     CDS             <1..723
                     /locus_tag="VN968_22675"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXL32072.1"
                     /translation="AQIKAASGLRRVALELGGNGVTIVHEDADIAEAAPVCARNSMRL
                     AGQSCISVQTVYVHRSVYEKFLDIVVTEVRKLRLGDPLDATTDVGTLIDEGAAQRVES
                     WIKEAADGGAKVLTGGRRHGAQIEPTVIAGVKPAMKVVCDEVFGPVISLLPYDDIDEV
                     FRTVSESRFGLQTGIFTKSMELAIRAVRNLRSGGVIINGSSTWRTDQLAYGGVKDSGI
                     GREGPRYSIRDMTEERMVLFNY"
     gene            837..1730
                     /locus_tag="VN968_22680"
     CDS             837..1730
                     /locus_tag="VN968_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HXL32073.1"
                     /translation="MGPRTKQKTSEKPASPRKDAVAELVQPPQGDIDDDADDRQRVGV
                     QSLGRAFAILEEVARHREGIGLADLSKLVDLHNSTTFHLAKTMVSLGYLRQERDSKRY
                     RVGRPLFALAASALDEIEMVNVATPVLEELSRETGESGHFAVRMGDAVVVIARTSGPG
                     AFQLTDRVGVVRPAHCTALGKIILASLRPDQLKRFLERVDMKPSTPKSITEIPVLLRE
                     IAEIKRSGIAFDDGEFNPEVRCVAVPVTDFTGQTIGAIGISGPIWRLSNQALHSSAQI
                     VQAAANRLSAEFGATAPAKSS"
     gene            1876..3114
                     /locus_tag="VN968_22685"
     CDS             1876..3114
                     /locus_tag="VN968_22685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32074.1"
                     /translation="MTRYNRRALLKAGAAFAGASAIGFPAISYGQAEKIKIGHLTPLT
                     GFLGALGTYATLGMKLAVEEINQGGGIMGRQLDIMSEDSVNPATAATKAQRMFEQDGA
                     TVLIGEINSAAALTIMQVAARNKRLFLQTGARSDALRGKNCNKFTFHMDIPNTVMVNT
                     VGKALVRDNMVKDKKFFTLTADYIFGHDLLAAAKRFFGANQGNLIGDELIATDVTDFS
                     PYLLKIRQAKPDVVCSNLAGNQVTTLIKQYAEFGLPYPIVGFNLNTADAWAAGDGNLS
                     GTWPTVWYHTLDVPASKRFVANFTRKNGKPPENHAWIEYVSFKMMAQAMGETKSTDTD
                     KLIAYFEKETEFDILKARKAHFRAWDHQLMQEAYPFTVRPKGHAKDQWDFIALGTALP
                     GAGEPLESLAPTREQNPCTL"
     gene            3209..4084
                     /locus_tag="VN968_22690"
     CDS             3209..4084
                     /locus_tag="VN968_22690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL32075.1"
                     /translation="MQFGFLLEQVVNGLVLGGYYLLIALGLSLIFSVGGVVNLAHGAF
                     YALGAYASVEITKYLGFGPAVVLSPIAVAMLGILFERYILRRFYTADPILSLLITFGL
                     AMIAEQAIRIAWGAAPLSASIPPVFRGSVIVGDFLFSRYRVMLLAVVAVVLIGVWLLL
                     HKTSFGRVVRAGIQRPDMVAALGIRLQPYMTAVVMLGVGMAAMGGAFFAPITIVHPAM
                     GNEIITVAFVVVVIGGLGSFWGVVIAALLVGVVRGITIHFMPAAGEASIYVLMFLVLL
                     VRPRGLLGERIEKFE"
     gene            4081..6621
                     /locus_tag="VN968_22695"
     CDS             4081..6621
                     /locus_tag="VN968_22695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HXL32076.1"
                     /translation="MMTASSSEKYRPLLFASLALIALPFVLKLLGLSLNTGTMVLTFA
                     IATMGLNLCVGYTGLVSFGHGTWFGIGAYAAGLIQLHWFGGEIWLPLLLSMAVVAATS
                     AVVGIIILRRRGVYFSLLTLAFAALTYTVAFRWSEVTGGEDGLGGLKRGSIGAFSLDN
                     VLAYYVTVALIALGVLYMLLRLVRSPFGHVLMAIRENQLRATFQGYPVERYKLGVFVI
                     SAVVTGLAGALLAFQTYLVSAEAVSVPFSGELLAMVVIGGMRSMLGPALGALFFILFR
                     ELFSIWTPNWLLWFGLIFVAFVMYSPGGLVGIWGTLSRRWWPAPEEGAAMSKRKIYQG
                     LPLPGFLMPEGLKGTVLEVNGISKHFGGIRAVTNASLAVGAGEIHALIGPNGAGKTTL
                     FNLISGLFTADGGTIRLNGREIQGVASDLICHRGLARSFQITNLFRELSIYENLRLSL
                     QAQHGIGFNFWRDIDSYPQVHAETAELIKFLGLEGTEQIEGGELSYGGQRLVDLGIAL
                     GSKPQVLLLDEPLAGLAAAERERVSSLIKNIAANIPVLIVEHDIDRVLGFSQAVTVMN
                     QGEVLMTGSPEAVRADRRVQEIYTGTGIPEVAHSRTDQAGGEAAQILRFEAVNTFYGK
                     SHILNDATLDVRQGEIVALLGRNGAGKSTLLKTLAGLVPLASGTIEYDGNNITGLPAP
                     DISRLGIGYVPQGRGLFAGMTVRENLSLGRLARKTDGGNGVVWSEEQILDYFPRLKER
                     LDVAADYLSGGEQQMVAVARAMSGNVKLLLLDEPFEGLAPTVILELFKVFDLLRRHTS
                     IVIVEHNLDLVLALADRVFALERGAVFHQGPAAPLLTDLEYRKKILWL"
     gene            6638..7594
                     /locus_tag="VN968_22700"
     CDS             6638..7594
                     /locus_tag="VN968_22700"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HXL32077.1"
                     /translation="MRSLVMGLMTGSMALSAAHAKAADEMTIAVFTKNQTNPAYEAFR
                     IAADRIAQATGVRVVHFVPKQPDDVDEQKAMVEQVLKDRPDVVIFIPVDDVAMIPSVK
                     ELNAANIPVVLVANPLPGSFVTYVGADDYEIGYRQAHYLFEALGGKGKIVVIEGTPAA
                     PTNRERVRGYKRAVAEYPGIMVLGSGNGNYQQPDAKRAMEKFLAAYPQIDAVLAANDG
                     MALGALEALKETGRTSVVIGINGILPAVQQIETGAILASVDFNMFKMGCTATRAAVRY
                     LKREPLPEKVILPAEIIDKTNYQAWLVPVEQRTCPEWAELAR"
     gene            9005..9241
                     /locus_tag="VN968_22705"
     CDS             9005..9241
                     /locus_tag="VN968_22705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32078.1"
                     /translation="MQKLAEPTEQEVNEGPQTVTFQIANGNARHHCILQTRLPTKVQA
                     QKYLLTNWPIIEKMARDALAAGTFEDGQIKLVMM"
     gene            9294..9473
                     /locus_tag="VN968_22710"
     CDS             9294..9473
                     /locus_tag="VN968_22710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32079.1"
                     /translation="MLNDRENILSALREKPLKVYEVMKRANVANEEACQSLLLKMRDE
                     GLVKFDIYKGRWLIG"
     gene            complement(9549..10319)
                     /locus_tag="VN968_22715"
     CDS             complement(9549..10319)
                     /locus_tag="VN968_22715"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32080.1"
                     /translation="MRDTVTPVEAAIHLDHVSKEFGKPGTEATYRAVDGLNLDVRPGQ
                     MLALLGKTGCGKSTIFNMIAGLTEPTSGTVRVSGRDPFSDFDSFRGRIAIVFQNDRLL
                     PWRTAIQNVELGLEMLDVAAAKRREAAQLWLTKLGLGGHENDYPHALSGGMRQRVSIA
                     RAFATNANILLCDEPFSALDESTAGRLRVEFVRLVKENGKTAVFITHSISEALEIGDR
                     IVVLKRPAMIAYDVSFDGGTGQEERELIRSRIGDVLAA"
     gene            complement(10316..11128)
                     /locus_tag="VN968_22720"
     CDS             complement(10316..11128)
                     /locus_tag="VN968_22720"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL32081.1"
                     /translation="MSETATQAGSSTAATLLAGTKRLGSSVLPFVVIIAIWQFASLFF
                     PRFLFPSLIDVFWRCLEIFSSGTMFADVLATILRILAGLAGAFIVGGLLALSMVRSRA
                     VDHFLSPILTLFQGIPALSWVVFAIIWFHGVEFRIFFIMVMTTLPAFTFQVLGALRAM
                     SRDLMEMVFSFRPTRIKLFRVMIAPAILPDILTAWKVNLGNASRVVVVAELVGATGGV
                     GYQLLQQQQLFDMAGALAWTLQLVFFVLIVQGALTLIENTAFRYRAVSERAI"
     gene            complement(11125..12096)
                     /locus_tag="VN968_22725"
     CDS             complement(11125..12096)
                     /locus_tag="VN968_22725"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32082.1"
                     /translation="MSNIRIGLACVSALLWAAPCFAETPEPISIVVFGPPSLGAFLPP
                     VIKAQKLDEKNGLAIKFEERTPDAYTAQFNSGEFQLGGSASLLTVGLADTRGVKVTYL
                     FNLFDYWGTVVTSRPEIKTLRDLEGKDLAAAKGTTNYVMFDWFARQQGADTAKFSVIN
                     TATPGLIGYAVADRAAAVQIWEPAYTTLLSKKSDMRTIDIGIAESWRKFTGSRNIPYL
                     GVAAHIDWVEKNPKLVAKLYAAYKEASEWVTAHPEEASKLIAPKGTPDDQKAITELIR
                     ANDRLGMSVQWASDVAKEISSVYAAGRSIAFLPADPAASTIYRASDK"
     gene            complement(12126..12593)
                     /locus_tag="VN968_22730"
     CDS             complement(12126..12593)
                     /locus_tag="VN968_22730"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HXL32083.1"
                     /translation="MKLDIGGVETIQAPVEVLWKALNDPVVLTRCIPGCKTMTEIAPD
                     AYKVEMQLRVAAVGGSFEGEIALSDKEPPKACSIKVSGAGTLGHGNGSARFQIEPEGP
                     DASRLAFQGAGEIGGLVAGVGQRILSSVSKHLVGRFFTALRKEFETPVEGAAE"
     gene            complement(12604..>13518)
                     /locus_tag="VN968_22735"
     CDS             complement(12604..>13518)
                     /locus_tag="VN968_22735"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL32084.1"
                     /translation="ASNFPSIRLIEDGGSLGFSFAGGKPTRPVSKPLSDLPARLKWMD
                     EQNVERQVVGGWLDMFGYEVPAEQGAAWSRLINTHLAQTAMREPRFVPLATVPLQDGA
                     RAAEVLREAQATGFKGAMIGTQPKGKGGVLDDPALKPFWEAANELGSVVFIHPVFESG
                     DDRVHDYGMANAVGRITDTLIAMSRLIYAGHVTRYANAKIVAGIGGAALPYVIGRLRR
                     NYSLDKDKLGDPDAALAAMYYDTIVQDPRALRYLADIVGADRIMMGSDMPFPIGDPAP
                     MKIVEETTFSDAERAAINGGLAQRLFGL"
BASE COUNT         2637 a         4229 c         4133 g         2519 t
ORIGIN      
        1 gcgcaaatca aggcggcatc gggcttgcgc cgggtcgcgc tcgaattggg cggcaacggc
       61 gtcaccatcg tccatgaaga tgccgatatc gccgaagcgg caccggtctg cgcccgcaac
      121 tcgatgcggc tcgccggtca aagctgcatc tcggtgcaga ctgtctatgt gcaccgcagc
      181 gtctatgaga aattcctcga tatcgtcgtc accgaggtca ggaaacttcg gctcggcgat
      241 cccttggatg ccacaaccga cgtcggcacg ctgatcgatg aaggcgcggc ccagcgagtg
      301 gaatcctgga tcaaggaggc cgccgatggc ggcgcaaagg tcctcaccgg cggcagacgc
      361 catggcgccc agatcgagcc gaccgtgatc gccggcgtca agccggcgat gaaagtggtg
      421 tgcgacgagg tgttcgggcc agtcatcagc ctgttgccct atgacgacat cgacgaggtt
      481 tttcgaaccg tcagcgaaag ccggttcggc ctgcagaccg gcattttcac caaatcgatg
      541 gagctcgcca ttcgtgcggt tcgcaacctg cgcagcggcg gcgtcatcat caacggcagc
      601 tcgacctggc gcaccgacca actcgcctat ggcggcgtga aggattcggg catcggccgc
      661 gagggtccgc ggtattcgat ccgggacatg accgaggaac gcatggtgtt gttcaattat
      721 taggtctgac taggtctgta tgagagcgcc cctgcacggg ggttgtatga acggccgccg
      781 cggccaatga tggggcggca gcacaattcg gcaccgtcgc gtggaggcta aatcctgtgg
      841 gaccgcgtac caagcaaaag accagtgaga agccggccag cccgcgcaag gacgccgtcg
      901 ccgagctggt gcaaccgccg cagggcgata tcgacgatga cgccgacgat cgccagcggg
      961 tcggcgttca gtcgctcggc cgcgcctttg ccatcctcga agaggtcgcg cgtcaccgcg
     1021 aaggtatcgg gctcgccgac ctgagcaagc tggtcgatct gcacaactcc accaccttcc
     1081 atctcgccaa gacgatggtg tcgctcggtt atctgcgcca ggaaagggat tcaaagcgct
     1141 accgcgtcgg ccggccgctg tttgccctgg cggccagcgc gctcgacgaa atcgagatgg
     1201 tcaatgtcgc aacgccggtg ctggaggagc tgtcgcggga aaccggcgaa agcggccatt
     1261 tcgcggtacg catgggcgac gccgtcgtcg tgatcgcccg caccagcggc cccggcgcat
     1321 ttcaactaac cgaccgcgtc ggcgtggtgc ggccggccca ttgcaccgcg cttggcaaga
     1381 tcatcctcgc ctcgctgcgc cccgaccaac tgaagcgttt tctcgagcgc gtcgacatga
     1441 aaccctcgac cccgaagtcg atcaccgaga ttccggtgct gctgcgcgaa attgccgaga
     1501 tcaaacgtag cggcatcgcc tttgacgatg gcgagttcaa tccggaagtg cgttgcgtcg
     1561 ccgtgccggt aacggatttt accggccaaa ccattggcgc gatcggtatt tccggtccga
     1621 tctggcgcct gtccaaccag gcgttgcaca gcagcgccca gatcgttcag gcggcggcca
     1681 atcgcctgtc ggctgaattc ggcgccacag ctccggcgaa atcttcgtaa acggagccga
     1741 gcggtacttt tggcgttgac agcgggcttc gcctccgaaa atatcatcca acaataaaaa
     1801 atcgcctctc acattctcgc atatgccgga attgagaggg acatcgccga tgcgcccttg
     1861 ggaaggagac caacgatgac ccgttacaac cgacgagccc tattgaaagc gggtgccgcc
     1921 tttgccggcg cctccgccat cggatttccg gccatcagct acggccaggc cgaaaagatc
     1981 aagatcggcc atctcacgcc gctgacaggt tttctcggcg cgctcggcac ctatgccacc
     2041 ctcggcatga aactggccgt ggaagaaatc aaccagggcg gcggcatcat ggggcgccag
     2101 ctggacatca tgtcggaaga ttcggtcaat cccgcgaccg ccgcgaccaa ggcccagcgc
     2161 atgttcgagc aagatggcgc taccgtgctg ataggcgaga tcaattcggc cgccgcgctg
     2221 accatcatgc aggtggccgc gcgcaacaag cggctgttcc tgcaaaccgg cgcacgatcc
     2281 gacgcgctgc gcggcaagaa ttgcaacaaa ttcaccttcc acatggatat cccgaacacg
     2341 gtgatggtga ataccgtcgg caaggcgctg gtgcgcgaca acatggtgaa agacaagaag
     2401 ttcttcacgc tgaccgccga ctatattttc ggccatgacc tgttggccgc ggccaagcgt
     2461 ttcttcggcg ccaaccaggg taacctgatc ggcgacgagt tgatcgcgac cgacgtgacc
     2521 gacttcagcc cctatctgct caagatccgc caggccaagc ccgacgtggt ctgttcaaac
     2581 ctggcaggca atcaggtgac gaccctgatc aagcaatatg ccgaattcgg cctgccctat
     2641 ccgatcgtcg gcttcaatct caatacggcg gatgcctggg cggcgggaga cggcaatctg
     2701 agcggcacct ggccgacggt ctggtatcac accctcgacg ttccggcctc caagcgtttc
     2761 gtggccaact tcaccaggaa gaacggcaag ccgcccgaga accatgcctg gatcgagtat
     2821 gtttcattca agatgatggc gcaggccatg ggcgagacca agtcgaccga caccgacaag
     2881 ctgatcgcct atttcgagaa agagaccgag ttcgacatcc tgaaggcgcg caaggcgcat
     2941 ttccgggcgt gggaccatca gctgatgcag gaagcctatc cgttcacggt caggcccaaa
     3001 ggccacgcca aggatcagtg ggacttcatt gcgctgggga ccgcgcttcc cggcgcaggc
     3061 gaaccgctgg agtcgttggc gccgacacgc gaacagaacc cctgcacgtt ataacggcgg
     3121 gtcgggaccc ggtgcgccca gttcagggcg cgccgggtgc ccgcgtattg ggcgcattgg
     3181 ctgacatcag agtctccctg gagcgagaat gcagttcggg ttcttgctgg aacaggtggt
     3241 aaacggcctc gtgctcggag gctattatct cttgatcgcg ctcgggctgt cgctgatctt
     3301 cagcgtcggc ggtgtggtga acctggcgca cggcgcgttt tacgcgctcg gcgcttacgc
     3361 atcggtcgaa atcaccaaat acctcggctt cggcccggcc gtggtgttgt cgccgatcgc
     3421 ggtcgcgatg ctgggcatcc tgtttgaacg gtacatcctg cgccgtttct acaccgccga
     3481 tccgatcctg agcctgctga tcaccttcgg tctcgccatg atcgccgaac aggcgatccg
     3541 tatcgcgtgg ggcgcggcac cattgtcggc atcgataccg ccggtgtttc gcggctcggt
     3601 catcgtcggc gatttcctgt tttcgcgcta ccgcgtgatg ctgctggcgg tggtggcggt
     3661 ggtgctgatc ggcgtctggc tgttgctgca caagacctcg ttcggccgcg tggtgcgggc
     3721 cggcattcag cggccggaca tggtcgcggc gctgggcatc aggctgcagc cctacatgac
     3781 cgccgtcgtc atgctcggcg tcggcatggc cgcgatgggc ggagcattct tcgcgccgat
     3841 caccatcgtg catccggcga tgggaaatga gatcatcacc gtcgcctttg tggttgtggt
     3901 gatcggcggc cttggcagtt tttggggcgt cgtgatcgcc gcattgctgg tgggcgtggt
     3961 gcgcggcatc acgattcatt tcatgcccgc cgccggcgag gcatcgatct acgtcttgat
     4021 gtttctggtg ttgctggtgc gtccgcgcgg gctgctcggc gagcgtatcg agaaattcga
     4081 atgatgaccg cctcatccag cgaaaaatac cgacccttgc tgttcgcaag cctcgcgttg
     4141 atcgctctgc cgttcgtcct gaagctgctc ggtctctcgc tgaacaccgg gaccatggtg
     4201 ttgacgtttg cgatcgcgac catggggctc aatctgtgtg tcggctacac cggactggta
     4261 tcgttcggcc acggcacctg gtttggcatc ggcgcctatg ccgcgggatt gatccagctg
     4321 cactggttcg gcggcgaaat ctggctgccg ctgctgctat cgatggccgt ggttgcggcc
     4381 acgtcggcgg tcgtgggcat catcatcttg cggcgccgcg gcgtctactt ctcgctgctg
     4441 acgctggcgt ttgccgcgct cacctacacc gtcgcgttcc gctggagcga ggtgaccggc
     4501 ggcgaagacg gtctgggcgg cctgaagcgc ggcagcatcg gcgcgttcag cctcgacaat
     4561 gtgctcgcct attatgtcac cgtcgcgctg atcgcgttgg gcgtgcttta tatgctgctt
     4621 cgcctggtgc gatcgccgtt cggccatgtg ctgatggcga tccgcgaaaa ccagctgcgc
     4681 gccaccttcc agggctatcc ggtcgaacgc tacaagctcg gtgtcttcgt gatctcggcc
     4741 gtggtgaccg ggctcgccgg cgcgctgctc gcgttccaga cctacctggt ttccgcagaa
     4801 gcggtttcgg taccgttttc cggcgaattg ctggcgatgg tggtgatcgg cggcatgcgc
     4861 agcatgctgg ggccggcgct cggcgcgttg ttctttatcc tgttccgcga attgttttcg
     4921 atctggacgc cgaactggct gctttggttc gggctgatct tcgtcgcctt cgtgatgtat
     4981 tcgcccggcg gcctggtcgg catctggggt acgctcagcc ggcgatggtg gcccgcgccg
     5041 gaagaaggcg ccgccatgag caagcgcaag atctaccaag gcctgccgct gccgggtttc
     5101 ctgatgccag agggcctcaa aggcaccgtg ctggaggtca atggcatctc aaaacatttc
     5161 ggcggcatcc gcgctgtcac caacgccagc cttgcagtcg gcgccggtga aatccatgcc
     5221 ctgatcggtc cgaacggcgc cggcaagacc acgctgttca acctgatctc cggcctgttc
     5281 acggcggacg gcggaacgat caggctcaac ggccgcgaga tccagggcgt ggcatccgat
     5341 ctcatctgcc accgcggact ggcgcgctcg tttcagatca ccaacctgtt ccgggagctg
     5401 tcgatctacg aaaatttgcg gctttcgctg caggcccagc acgggatcgg cttcaacttc
     5461 tggcgcgaca tcgacagcta tccgcaagtg cacgccgaaa ccgcggagct gataaagttc
     5521 ctcggcctcg aaggcaccga gcagatcgag ggcggcgaac tgtcctatgg cgggcagcgg
     5581 ctggtggatc tgggcatcgc tctcggctcc aagccacagg tgctgttgct cgatgagccg
     5641 ctggccggtc tcgccgccgc cgagcgtgag cgggtgtcga gcctgatcaa gaacatcgcc
     5701 gcgaatattc cggtcctgat cgtcgaacac gacatcgatc gcgtgcttgg tttttcgcaa
     5761 gccgtcaccg tgatgaacca gggcgaagtg ctgatgacgg gttcgccgga ggcggtacgc
     5821 gccgaccggc gcgtgcagga aatctatacc ggtaccggca tccccgaggt cgcacacagc
     5881 cgcaccgatc aggccggcgg ggaagcggcg caaatcctgc gtttcgaagc cgtcaacacc
     5941 ttctacggca agagccacat tctgaacgac gcgaccctgg atgtgcgcca aggcgagatc
     6001 gtcgcactac tggggcgcaa cggcgccggc aagtccacgc ttctgaaaac gctggccggg
     6061 ctggtgccgc tggcctccgg caccatcgaa tatgacggca ataatatcac cgggctgccg
     6121 gccccggata tttcgcgcct ggggatcggc tacgtgccgc aggggcgcgg cctgttcgca
     6181 ggcatgacgg tgcgggaaaa tctttcgctt ggccgtctgg cgcgcaagac cgacggcggc
     6241 aacggcgtgg tgtggagcga ggagcaaatc ctcgactatt tcccgcgcct gaaggagcgg
     6301 ctggatgtcg cggcggatta cctgtccggc ggcgagcagc aaatggtggc ggtggcgcgt
     6361 gcgatgtcgg gcaacgtcaa attgttgctg ctggatgagc cgttcgaggg cctcgccccg
     6421 accgtgatcc tggaactgtt caaggtgttc gacctgctgc gccgacatac atccatcgtg
     6481 atcgtggagc ataatctgga tctggtgctg gcgctggccg accgcgtgtt cgcgctcgag
     6541 cgcggcgccg tgtttcacca ggggccggcg gcgccgttgt tgaccgatct ggaataccgc
     6601 aaaaagatcc tgtggctgtg acctcaagga ggcgacgatg cgaagccttg taatgggact
     6661 gatgaccggc tcaatggcgt tgtccgccgc tcacgccaaa gcagcggatg agatgaccat
     6721 cgccgtgttc accaaaaacc agaccaatcc ggcctatgaa gcgttccgca tcgccgccga
     6781 ccggattgcg caggcgacgg gcgtgcgggt ggtacacttc gttcccaagc agccggacga
     6841 tgtcgacgaa cagaaggcga tggtggagca ggtcctcaag gacaggccgg acgtagtcat
     6901 cttcatcccc gtcgatgacg tcgcgatgat cccgtcggtg aaagaactca acgcggcgaa
     6961 catcccggtc gtgctggttg ccaatccgct ccccggcagt tttgtcacct atgtcggcgc
     7021 cgacgattac gagatcggat accggcaagc gcactatctg ttcgaggcgc tcggcggcaa
     7081 gggaaagatc gtcgtcatcg aaggcacccc ggcggcgccg accaatcgcg aaagggtgcg
     7141 cggctacaaa cgcgccgtcg ccgaatatcc cggtatcatg gttcttggct ccggcaacgg
     7201 caactatcag caacccgacg ccaaacgcgc gatggagaaa ttcctggctg cctatccgca
     7261 aattgacgcc gtgctcgcgg ccaacgacgg catggcgctt ggcgccttgg aggcgctgaa
     7321 ggaaacgggc cggacatccg tcgtgatcgg catcaacggt attctgccgg ccgtccagca
     7381 gatcgagacc ggcgcgatcc tcgcgtccgt cgatttcaac atgttcaaga tgggctgcac
     7441 ggcgacccgc gccgcggtgc gctacctcaa gcgtgagccg ctgccggaaa aagtcatcct
     7501 gccggccgag atcatcgaca agaccaacta ccaggcctgg ctcgtcccgg tggagcagcg
     7561 tacctgcccg gaatgggccg aactggcgag gtgagagaac gaagggtagg cacgctgcgc
     7621 tttgcccatg acctctcatc gtcgtccccg cctagtgcgc aattgtgcac caggcgggga
     7681 cgacgcgtga tgtcgcgttg gcaagcaatg aactacctcg gattcgattt tttcaaacag
     7741 tccagcaatc tcacaatttc agacacagct tcgcggcctc accgcgcaaa acgcccgagt
     7801 catgcattta actctcgccc cctttgagac cccctttgag ggcgtgggga atgccggatg
     7861 cccgctgcac ccgcggtctc gtgtgcactt tgtattggta gaacgcacac gagtaacaac
     7921 gagtccaccg ggatcacccg gcattcccgc acgcaatggt tttacggctt atgtcgtgct
     7981 ctccccggtg accgggctct cttgccaccg tcatccgcag agcaaggttt tgtcactgcc
     8041 cggtagggcc aacaaaactc cgcgaacttg acgccagcgt cggggcgtca ggaccacacg
     8101 attttgccgt ccgcagcaac atttctcgtc agcgtgctct ttgatcgctc acggattcaa
     8161 gaacccgccc tgcgatccca tcgcgcgcaa aatgctgccg cgtccaccgc atcccgcccc
     8221 tacgtccatg acgatcgcga aacgcccctc tgtgtgggac gggatgcgaa aggttctaga
     8281 tgtgatttgg ggggtacgaa aacagaaata tttttgcaaa gggggctgga cacgcctgtc
     8341 aacaaaccgc ccgacgggca aatcaccaca cgactgcgaa gagcaatttc ctctcgtgtc
     8401 ccggacgcgg agcagcgccc ttcgcgctgc tccgcagagc cgggaaatcc cgaatgtcgc
     8461 tccgctcatc taggccacgc ctgcttgatc acatgctgag tatccaatca gcgaaaccaa
     8521 atccagagcg ccatgccggc gatcagcgcg cgagctgcta caatcgcgta agaaggaatg
     8581 tccaatgtga agacaaactc gctatgtgca tcaggcaagt agtgcattgc cacgccccca
     8641 gagctccgat tgtcaaatgg ctaatgccca tatttttgtg atcgcccctg gcatttactt
     8701 ttgcctcgag catccccaca tagtgtccat ggctaactgg caacaatcta atttcacacg
     8761 ggaaccgccg aagacaaagg cggagctacg ccagatgctg gccgaggccg tccgcaacac
     8821 gcaacccgac gcggaccacg ggccgaagcg cctgccaaag gccaaaagga tcgcggccgc
     8881 ggcggccggc gtcgattaaa tctgccgggc cacaggtatc cacctccctt tccgcagcca
     8941 attcaccgaa gatacaagcg acttcattcg cgacccgcgc gatagtgtca taaaggaaca
     9001 gtgtatgcag aaacttgccg agcccaccga acaagaggtc aacgagggac cgcaaacggt
     9061 aacctttcag atcgcgaatg ggaacgcgcg gcaccactgc atcctgcaaa ccaggctacc
     9121 gacgaaggta caggcgcaga agtaccttct cacaaattgg ccgatcatcg aaaagatggc
     9181 acgcgacgcg cttgcggcgg gaaccttcga agacggccag atcaagcttg taatgatgta
     9241 aacggcttat tcgagcgtga ggatcgggat ttcctggttt gtggggagca gtcgtgctga
     9301 atgatcgaga aaatatcctg agcgcgctcc gcgagaagcc gctcaaggtc tacgaggtca
     9361 tgaaacgggc caatgtcgcg aatgaggaag cctgccaatc cctcctgttg aaaatgcgag
     9421 acgaaggcct ggtgaaattc gatatttaca agggacgatg gctgatcgga tgatccacga
     9481 aatggccgcg agagcagcac ctgcgccgct cctcgcgcca tggtcgccgg cttgacgcca
     9541 acgccaactc acgccgcgag cacgtcgccg atccggcttc gaatgagctc gcgctcttcc
     9601 tggccggtgc cgccgtcaaa actgacatcg taggcgatca tcgccggacg cttcagcacc
     9661 acgatgcggt cgccgatttc cagcgcttcg ctgatcgaat gggtgatgaa gacggcggtc
     9721 ttgccgtttt ccttcacgag gcgaacgaat tccacgcgca gccgcccggc cgtggattca
     9781 tccagcgccg agaacggctc gtcgcacaag aggatattgg cattggttgc aaacgcacgc
     9841 gcgatcgaga cacgctgacg catgccgccg gacagcgcgt gcggataatc gttctcgtgc
     9901 ccgccaagtc cgagctttgt cagccacagc tgcgccgcct ctcgccgctt cgccgccgct
     9961 acgtccagca tctccaggcc cagttcgacg ttctggatgg cggttcgcca gggcagcagc
    10021 cggtcgttct gaaacacaat cgcgattctg ccgcgaaacg aatcgaagtc cgaaaacgga
    10081 tcgcgcccgc tgacgcgcac ggtgccgctg gtcggctcgg tcagcccggc aatcatgttg
    10141 aagatcgtcg acttgccgca gccggtcttg ccgagcaacg ccagcatctg gccggggcga
    10201 acgtcgagat tgagaccgtc caccgcacgg taggtcgctt ccgtgcccgg cttgccaaat
    10261 tccttggaaa catggtcgag gtgaatggcc gcctcgaccg gcgtgacggt atccctcata
    10321 tcgcacgctc cgaaaccgcc cgatagcgga aggcggtatt ttcgatcagc gtcagcgcgc
    10381 cctgcacgat cagcacgaaa aacaccagtt gcagcgtcca ggccagggcg ccagccatgt
    10441 cgaacagctg ctgctgctgc agcaactggt agccgacgcc gccggtggca ccgaccagtt
    10501 cggcgaccac gaccacgcgc gaggcgttgc cgaggttgac cttccacgcc gtcagaatgt
    10561 ccggcaggat cgccggcgcg atcatcaccc gaaacagttt gatgcgcgtc gggcgaaagc
    10621 tgaacaccat ctccatcaga tccctggaca tcgcgcgcaa cgcgccgagc acctgaaacg
    10681 taaacgccgg cagcgtcgtc atcaccatga tgaagaagat ccgaaactcg acgccatgaa
    10741 accagatgat ggcgaatacc acccaggaca gcgcgggaat cccttggaac agggtcagga
    10801 tcggcgagag gaaatgatcg accgcacgcg agcgcaccat cgacagcgcg agcaagccgc
    10861 cgacaatgaa ggcgccggca agtcccgcca gaatgcgcag gatcgtcgcc agcacatcgg
    10921 cgaacatcgt cccgctcgaa aagatttcaa gacagcgcca gaacacgtcg atcagcgacg
    10981 gaaacagaaa gcgcgggaaa aacagcgagg cgaattgcca gatcgcgatg atgacgacga
    11041 aaggcagcac cgacgatccc agccgttttg tcccggccag cagcgtcgcg gccgtcgacg
    11101 atccggcctg ggttgcggtc tcgctcattt gtcggacgcc cggtagatcg tcgaggcggc
    11161 gggatcggcg ggaaggaagg cgatcgatct gccggcggcg tacaccgagc tgatttcctt
    11221 ggcgacgtcg gaggcccact gcacgctcat gccgagccgg tcattggcgc gaatgagttc
    11281 ggtaatcgcc ttctgatcgt cgggcgtccc cttgggcgcg atcagcttcg acgcctcctc
    11341 gggatgcgcc gttacccatt ccgaggcttc cttgtaggcg gcgtaaagtt ttgcaaccag
    11401 ctttgggttc ttttccaccc aatcgatatg ggcggcgacg ccgagatagg gaatgttgcg
    11461 gctgccggtg aatttcctcc agctctcggc aatgccgata tcgatcgttc gcatatcgct
    11521 cttcttcgac agcagcgtgg tataggccgg ctcccagatt tgcacggccg ccgcgcgatc
    11581 ggccacggca tagccgatca ggccgggggt cgcggtattg atcaccgaaa attttgcggt
    11641 gtcggcgccc tgttgccggg cgaaccaatc gaacatcaca taattggtgg tgccctttgc
    11701 cgcggcgagg tccttgccct ccagatcccg cagcgtcttg atctcgggcc gggaggtgac
    11761 gaccgttccc caatagtcga acagattgaa aagataagtc accttgacgc cgcgggtatc
    11821 ggcaaggccg accgtcagca acgaggcgct gccgccaagc tgaaactcgc cggagttgaa
    11881 ttgcgcggta taggcgtcgg gcgtgcgttc ctcgaatttg atcgcaaggc cgttcttctc
    11941 gtccagtttt tgcgccttga tcaccggggg caggaacgcg ccgagcgatg gcgggccgaa
    12001 gacgacaatc gaaatcggct ccggcgtttc ggcaaagcaa ggcgcagccc acaacaatgc
    12061 cgagacgcaa gcaaggccga tacgaatgtt actcatggcc gtcctttcag gaattctcgt
    12121 caaagctatt ccgcggcgcc ctcaaccggt gtttcgaatt ccttgcgcag cgccgtaaag
    12181 aaacgaccga ccagatgctt ggagacactg ctcaagatcc tctggccgac gccggcgacc
    12241 agtcccccga tttcgccggc gccctgaaac gccaggcgcg acgcgtcagg cccttccggc
    12301 tcgatctgga agcgcgcgct gccgttgcca tggccgagcg tgccggcgcc ggacaccttg
    12361 atgctgcagg ccttcggcgg ctccttgtcc gacagggcga tctcgccttc gaatgaaccg
    12421 cccaccgccg cgacgcgcag ctgcatctcc accttgtagg catcgggcgc gatctcggtc
    12481 atggtcttgc agccggggat gcagcgcgtc agcacgacgg gatcgttgag cgccttccag
    12541 agcacttcaa ccggagcctg aatggtttcg acaccaccga tatcgagctt catggatatg
    12601 ccctcacaat ccgaacaaac gttgcgccag cccgccattg atcgcggcgc gctcggcatc
    12661 cgaaaatgtt gtctcctcga caattttcat cggcgcggga tcgccgatcg ggaacggcat
    12721 gtccgacccc atcatgatcc ggtcggcacc gacgatgtcg gcgagatagc gcagcgcccg
    12781 cgggtcctgc acgatggtgt cgtaatacat cgcggccagc gccgcatcgg gatcgccgag
    12841 cttgtccttg tcgagcgaat agtttcggcg caggcggccg atcacataag gcagcgccgc
    12901 gccaccgatt ccagccacga tcttggcgtt ggcataacgg gtgacgtgac cggcatagat
    12961 cagccgcgac atcgcaatca gcgtatcggt gatgcgtccc acggcattcg ccatgccgta
    13021 atcatggacg cggtcgtcgc cgctttcgaa taccggatgg atgaagacca ccgatccgag
    13081 ttcgttggcg gcttcccaga acggttttag cgcggggtca tcgagcacgc cgcccttgcc
    13141 cttgggctgg gtaccgatca tcgcgccctt gaagcctgtg gcctgcgctt cgcgcagcac
    13201 ttcggcggcg cgcgcgccgt cctgcaaggg gacggtggcc agcggcacaa accgtggctc
    13261 gcgcatcgcg gtttgcgcca gatgggtatt gatcaatcgc gaccatgccg cgccctgctc
    13321 cgccggcact tcatagccaa acatatcgag ccagccgcca accacctgac gctcgacgtt
    13381 ctgctcgtcc atccatttca gccgcgccgg cagatcgctc agcggctttg aaaccggccg
    13441 cgtcggcttg ccgccggcaa acgagaaacc gaggctgccg ccgtcttcga tcaggcggat
    13501 cgagggaaaa ttcgaagc
//
LOCUS       DATNOI010000563         6246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_241622,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000563 DATNOI010000000
VERSION     DATNOI010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6246
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241622"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1942
                     /locus_tag="VN968_22740"
     CDS             <1..1942
                     /locus_tag="VN968_22740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HXL32085.1"
                     /translation="ACRVIRTAKAMGLRTVAVYSEADRDTMHVAMADEAVLLGPARAR
                     DSYLNIERVIDAARQSGAEAVHPGYGFLSENAEFAQACADAGLVFVGPTAAMMTAMGS
                     KSGSKMLMEKAGVPLVPGYHGDAQDEATLAKAADKIGFPVLIKASAGGGGRGMRIVSS
                     SSELAAAITSAKREAKAAFGDDRLLIEKFVENPRHIEVQIIGDSHGNLLSLFERECTL
                     QRRHQKVVEEAPSPTLNPAQREAVCAAARKAAAAVNYAGAGTIEFVSDGKDVFFIEMN
                     TRLQVEHPVTELITGIDLVDWQLRVAFGENLPLNQDQISFNGHAIEARVYAENPHKNF
                     MPSVGRIRTWRTPEQSNGLRIDAGYREGDAVSPHYDAMLAKVIAWAPTRDAAIEKLNR
                     GLEETDVRGIVTNIAFLSALLTHPDVRANAIDTGFIERELKKLAPPPAAPGDLELCAA
                     VAAILDDEAKVHAEAHSPWRTAGWMPVGRRQRLFAFRDRQGAERKITLKYGNGPATLS
                     IDARDFAFTCSPALDGGFDLTLDGMKSHILAVLEGHELYLRTRNGRFELHWVDPFGGD
                     DEEQVGEDKIVAPLPGTVVALLAEEGARLEKGAAILTLEVMKMEQTLRAPFAGVLKLI
                     KCKVGDIVQEGVELAEVEPLGS"
     gene            1966..2880
                     /locus_tag="VN968_22745"
     CDS             1966..2880
                     /locus_tag="VN968_22745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HXL32086.1"
                     /translation="MSEKVRIVEVGPRDGLQNEKTPVSVEARIAFIEALVGAGLRTVE
                     VGAFVSPKAIPQMVNSDAVLRGVNHQPDSEFHVLVPNEKGYAAARAAGAKVIAVFASA
                     SEGFSRANINCSIAESIERFKPVIARAKADGVKVRGYISCVLGCPYDGEVKPQAVVDV
                     AKTLWDLGCYEISLGDTIGVGTPVKARQLLRAVAGAVPMVDLAMHFHDTYGQALANLY
                     AGMEEGARVIDSAAGGLGGCPYAPGATGNVATEDVVYMLEGMGIATGVDMAKLVAATN
                     EVSRLIGRPPVSRVASALNAKMRVTNNK"
     gene            complement(2911..4269)
                     /locus_tag="VN968_22750"
     CDS             complement(2911..4269)
                     /locus_tag="VN968_22750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXL32087.1"
                     /translation="MTVLGIGISYGLATLFAMFSGMPIAFALGAVAVVFMGIYMPAAS
                     LDTVTQNVYEEMASITLLSIPLFILKGAAIGKSRAGQDLYSALHAWLHRVPGGLGVAN
                     VFACALFAAMAGSSPATCSAIGSAGIPEMRKRGYSGGFAAGIIAAGGTLGILLPPSIT
                     MILFAVAAEKSLGRLFLAGIGPGLLLVTLFGGYAVIRFRIEYAAAKTVYTKTGATSAI
                     LTRDDFTMAERFNVLPRVLPFVLLLTGVMIALYGGYATPSETAGLGGLLALALIALIY
                     SVWRPSDLAPIMKSTIRDSTMLMMIIGMSLLYSYVMSYLHISQSVAESIVAMHLPRWE
                     LLAAILVMVVVLGFFLPPVSIILMTAPIILPPLRAANFDIIWFGVVMTIVMEMGLIHP
                     PVGLNIFVIRNVAPDIPLMEVIWGTLPFVLLMMAAVVVLCFLPEIATYLPDLVMGPDG
                     GR"
     gene            complement(4266..4820)
                     /locus_tag="VN968_22755"
     CDS             complement(4266..4820)
                     /locus_tag="VN968_22755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXL32088.1"
                     /translation="MMHGPLPDQNDHLDTATSNALVAALQRGLAFCNYIIVVLAAVAL
                     IAACVILSYSVLSRALFHSPNYWQDEAAVFLLVGATFMTAAYVQGQRGHIGIEAFVGL
                     LSPMVNRARLWLVDLASLLFCAFFAWKSWTLAHEAWVDGQVSNSMWSPPLAIPYGLMA
                     AGMTLLCLQILLQLTIPLGGLARR"
     gene            complement(4817..5839)
                     /gene="dctP"
                     /locus_tag="VN968_22760"
     CDS             complement(4817..5839)
                     /gene="dctP"
                     /locus_tag="VN968_22760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HXL32089.1"
                     /translation="MLTRRNVLVTALAAPAILRFGTGTARAATTLKISHQFPGGTIDK
                     GDFRDRLCRIFAAEVAKRSGGEIAAEIYPNSSLIKTNAQFSAMRKGALDISLYPLPYA
                     GGELPETNIGLMPGLVSTYDQGLRWKNQPVGKALTDFLADKGIVILTWIWQAGGVASR
                     SKPLVVPADAKGLKVRGGSREMDMVLQTAGAAVLSLPSNELYAAMQTGACDAGLTSST
                     SLISFRLEEVAKHLTSGAGASYWFMLEPLLMSKSIFDKLPKNHQDIIRAVGTELEAFG
                     RKGAQDDDIEVAKVYEKAGAKVSPLDVATVGKWRDIAQDTAWKDYAGKTAAAANLLKL
                     ATDVSA"
BASE COUNT         1244 a         1917 c         1975 g         1110 t
ORIGIN      
        1 cgcctgccgc gtgatacgca cggcgaaggc gatgggcctg cgcacggttg cggtttactc
       61 cgaggccgac cgcgacacca tgcatgtcgc gatggctgac gaggccgtgt tgctcggacc
      121 ggcgcgggcg cgcgatagtt acctgaatat tgaacgcgtc attgacgctg cgcggcagag
      181 cggagcggaa gccgttcatc ccggctatgg atttctttcg gagaatgccg aattcgcgca
      241 agcctgcgcc gatgccggac tggtattcgt cggccccacc gccgccatga tgaccgcaat
      301 gggctcaaaa tcgggctcga aaatgctgat ggaaaaggcc ggcgtgccgc tggtgccggg
      361 ctatcacggc gacgcccagg acgaggcgac acttgccaag gccgccgaca agatcggttt
      421 tcccgttctg atcaaggcat cggcgggtgg cggcggtcgc ggcatgcgga tcgtgagttc
      481 gtccagtgag cttgccgcgg cgatcacaag cgccaagcgc gaggccaagg cggccttcgg
      541 cgacgaccgc ttgctgatcg aaaaattcgt cgaaaaccca cgccacatcg aggtgcagat
      601 catcggcgac agtcacggca atctgctgtc gctgttcgag cgtgaatgta ccttgcagcg
      661 gcggcatcag aaggtagtcg aggaagcccc gtccccgacg cttaatcccg cgcagcgcga
      721 ggcggtatgc gccgccgcgc gcaaggcagc cgccgcggta aattacgccg gtgccggcac
      781 catcgagttt gtttcggacg gcaaggacgt cttcttcatc gagatgaata cccgcctcca
      841 ggtcgagcat cccgtgaccg agctgatcac gggcatcgat ctggtggatt ggcagctgcg
      901 cgtcgcgttc ggcgaaaacc tgccgctcaa ccaggatcaa atcagtttca acggccacgc
      961 catcgaggcg cgagtttacg cggaaaatcc acacaagaat ttcatgccgt cggtcggccg
     1021 catcagaacc tggcgcacgc cggagcagtc gaacgggctg cgcattgatg ccggctaccg
     1081 cgagggcgat gcggtatcgc cgcactacga cgccatgctg gccaaggtga tcgcgtgggc
     1141 gccgacccgc gacgcggcga tcgagaagct taatcgcggg cttgaggaaa ccgatgttcg
     1201 cggcatcgtc accaacattg cctttctttc ggctctgttg acgcatccgg acgtgcgcgc
     1261 caatgccatc gataccggat tcatcgagcg tgaattgaag aagctggcgc cgccccctgc
     1321 ggcgcccggc gatctcgagc tttgcgccgc cgtggcggca atcctcgacg acgaagcaaa
     1381 agtccacgcg gaggcgcatt cgccgtggcg gaccgcgggc tggatgccgg tcggtcgccg
     1441 gcagcggctg ttcgcgttcc gcgacaggca gggagccgaa cgaaagataa ccctgaaata
     1501 cggcaacggc cccgcgacgc tgtcgatcga cgcgcgtgac ttcgccttca cctgttcgcc
     1561 cgcccttgat gggggattcg acctcacgct cgacggcatg aaatcccaca ttctcgctgt
     1621 gctcgaaggc catgaactgt acctgcggac gcgcaacggc cgtttcgagc tgcattgggt
     1681 tgatccgttc ggcggcgacg acgaagagca ggtcggtgaa gacaagatcg tagcgccctt
     1741 gcccggcacc gtagtggcgt tgctggccga ggagggggcc aggctggaga agggcgcggc
     1801 gatcctgacg ctcgaggtca tgaagatgga gcagaccctg cgcgcgccat tcgcgggcgt
     1861 gctgaaactg ataaagtgca aggtcggcga tatcgtgcag gaaggcgtcg aactcgccga
     1921 ggtcgagccc ctcggttcct aggattttgg cttcgtgaaa ccaccatgag cgaaaaagtg
     1981 cgcatcgtcg aagttggacc gcgggacggg ctgcaaaacg agaagacgcc ggttagtgtc
     2041 gaagcccgca tcgcgttcat cgaggcgctg gttggcgccg ggctgcgtac cgttgaggtc
     2101 ggcgcctttg tgtcgccgaa ggcgatcccg cagatggtga attcggatgc cgtgctgcgc
     2161 ggcgtcaacc atcaacccga cagcgagttc cacgtgctgg tgccgaacga aaagggttac
     2221 gcggccgcgc gcgcggccgg cgccaaggtg atcgcggttt ttgcttcggc ctcggaaggc
     2281 ttttcacgcg ccaatatcaa ttgctcgatc gccgaatcga tcgagcgctt caaaccggtg
     2341 atcgcccgcg ccaaggccga cggcgtcaag gtgcgcggct atatctcctg cgtgctcggc
     2401 tgtccctatg acggcgaggt caagccgcag gcggttgtgg acgtcgcgaa gactctttgg
     2461 gatctcggct gttacgaaat ttcgctcggc gacaccatcg gggtcggcac ccccgtcaag
     2521 gcacggcagt tgctgcgcgc cgtggccggc gccgtgccaa tggtcgatct cgccatgcat
     2581 tttcacgaca cttacgggca ggcgctggcc aatctctatg ccgggatgga ggaaggcgcg
     2641 cgcgtgatcg attccgcggc cggcggtctc ggcggctgcc cctacgcccc cggcgcgacc
     2701 ggcaatgtcg ccaccgaaga cgtggtctac atgctggagg gcatgggcat tgcgaccggc
     2761 gtcgatatgg caaaactggt ggcggccacc aacgaagtga gccggttgat cggccggccg
     2821 ccggtgagcc gcgtcgcctc ggcgttgaac gcgaagatgc gtgtaaccaa taacaagtag
     2881 cgaatggaag ttgccgtgtt cgccgctacg ttacctgccc ccatccggtc ccatcacgag
     2941 atcgggcagg tacgtcgcaa tttccggcag gaagcacagc accacgacgg cggccatcat
     3001 cagaagcacg aatggcagcg tgccccagat cacttccatt agcggaatat ccggcgcgac
     3061 gttgcggatg acgaaaatgt tcaagcccac cggcggatga atcagtccca tctccatcac
     3121 gatggtcatg acgacgccga accagatgat gtcgaagttt gcggcccgga gcggcggcag
     3181 gatgatcggc gcggtcatca gaatgatcga aaccggcggc agaaaaaagc cgagcaccac
     3241 gaccatgacg aggatcgccg ccagcaattc ccagcgcggc agatgcatcg cgacgatcga
     3301 ttctgcgacg gattgggaga tgtgcaggta gctcatcacg tacgagtaca atagcgacat
     3361 gccgatgatc atcatcagca tggtggagtc gcgtatcgtc gatttcatga tcggcgccag
     3421 gtcgctcggc cgccacacgc tgtagatcag cgcgatcagc gccagtgcca gcagcccgcc
     3481 gaggcctgcg gtttcggacg gcgttgcata gccgccgtaa agcgcaatca tcaccccggt
     3541 cagcaacaag acgaacggca gcacccgcgg cagaacattg aaccgctccg ccatggtaaa
     3601 atcgtcgcgt gtgaggatcg ccgaggtcgc gccggttttg gtatagaccg tcttggcggc
     3661 tgcatattct attcgaaagc ggatcaccgc atagccgccg aacaaggtca ccagcagcag
     3721 gccgggcccg atgccggcaa gaaacagccg tcccagtgat ttttccgccg cgaccgcgaa
     3781 caggatcatg gtgattgaag gcggcagcaa tatgccgagc gtgccgccgg cggctatgat
     3841 cccggccgcg aagccgccgg aatagccccg cttgcgcatc tcggggatgc cggccgagcc
     3901 gatcgccgag caggtggcgg gcgatgatcc cgccatcgca gcgaacaacg cgcacgcaaa
     3961 cacgttggcg accccaagcc cgccgggcac gcgatgcagc caggcgtgca gcgctgaata
     4021 caaatcctgc ccagcgcggg atttcccgat cgcggcgccc ttgaggatga acagcgggat
     4081 cgacaacagc gtgatcgagg ccatttcctc atagacattc tgggtcaccg tatcgagcga
     4141 ggccgcgggc atgtagatgc ccatgaacac cactgcgact gcgcccagcg caaatgcaat
     4201 cggcatgccc gagaacatgg caaaaagggt ggcgaggccg tagctgatgc cgattccgag
     4261 cacggtcatc ggcgcgccag tccgccgagc ggaatcgtca gctgcagcag gatctgcagg
     4321 caaagcagcg tcatgccggc cgccatcagg ccgtagggaa tcgccagcgg cggcgaccac
     4381 atcgagttcg acacctggcc gtcgacccag gcttcatgcg ccagtgtcca ggatttccag
     4441 gcgaagaacg cgcaaaacaa aaggctcgcc aggtcgacga gccacagcct ggcgcggttg
     4501 accatcggcg atagcagtcc gacgaatgcc tcgatgccga tgtggccgcg ttgcccctgc
     4561 acataggccg ctgtcatgaa ggtcgcacca accagcagga acactgccgc ctcgtcctgc
     4621 caataattcg ggctgtgaaa cagcgcgcgg ctcagcacgc tgtagcttag gatgacgcag
     4681 gccgcgatca gcgcgacggc cgcgaggacc acaatgatgt agttgcaaaa ggccagcccg
     4741 cgttgaagcg ccgccacgag cgcattgctg gtggcggtgt ccaaatggtc attctgatcc
     4801 ggcagcggac catgcatcat gcggagacgt cggtcgcgag cttgagcagg ttggccgcgg
     4861 cagcggtctt tccagcgtag tccttccacg cggtgtcctg cgcgatgtcg cgccatttgc
     4921 ccacagtggc cacgtcaagc gggctgacct tggcgccggc cttctcgtag actttagcca
     4981 cttcgatgtc atcgtcctgc gcgcccttcc ggccgaacgc ttccagttcg gtgccgacgg
     5041 cccgaatgat gtcttggtga tttttcggca gcttgtcgaa gatcgacttc gacatcaaaa
     5101 gcggctccag catgaaccag taggatgcgc ccgcacccga cgtgaggtgc ttcgcaactt
     5161 cctcaagccg gaacgagatc aggctggtgg aggaggtgag gccggcgtcg catgctccgg
     5221 tctgcatcgc cgcatagagt tcgttggacg gcagcgacag cacggctgcg cccgcagtct
     5281 gcagcaccat gtccatctcg cgcgagcccc cgcgtacctt gaggcccttg gcgtctgccg
     5341 ggacgacgag tggcttggaa cggcttgcga cgccgccggc ctgccagatc caggtgagga
     5401 tgacgatgcc cttgtcggcc agaaaatccg tcagtgcctt gccgaccggt tggttcttcc
     5461 agcgcaaacc ctgatcgtag gtcgagacca gaccgggcat caggccgata ttggtttccg
     5521 gcagttcgcc gcccgcgtag ggaaggggat aaaggctgat atccagcgcg cctttgcgca
     5581 tcgccgagaa ttgcgcgttg gtcttgatca gcgaggaatt cggatagatc tcggcagcaa
     5641 tttcgccgcc gcttcgcttg gctacttccg cggcgaatat cctgcatagc cggtcacgga
     5701 aatcgccctt gtcgatggtg cctcccggaa actggtgcga tattttcaat gtggttgcgg
     5761 ctcgggccgt tcctgtccca aaacgcaaaa tggccggcgc ggcaagcgct gttacgagga
     5821 cgttacggcg tgtgagcatg gatatctccg ttaagctatt cttaatcaga ttgttcgggc
     5881 tggggttgcg gcatattagc ccgtcttcat caggactttc tctagtctga tagttcgagg
     5941 tcgccctggt gtttaacgcc cggcttgttg cggcgcacac ttgcatcaaa gatgcgaccg
     6001 aaaaaagacc gaccccgcgg gttctagcga gcattcactg cgaaccccga gactgcttga
     6061 aaaagccccc tcacaaaaaa gttttcaaac agccggtaac aaacgaggtc gcccgaccgt
     6121 ggtgaacgta gcggcatcag tcgctttcga aaccggctca aaggaagacc aacccatgac
     6181 cattcgcacc cgcactgctc tcggcgtttc tgctgccgtt ctttcgctca gcctggcgct
     6241 ggcgcc
//
LOCUS       DATNOI010000564         4452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1691651,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000564 DATNOI010000000
VERSION     DATNOI010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691651"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..468)
                     /locus_tag="VN968_22765"
     CDS             complement(85..468)
                     /locus_tag="VN968_22765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HXL32090.1"
                     /translation="MSDEGRDRFEIPKDMRSMAEASFEQARKAFDKFLTSAQQTAGSL
                     EEQGATVRAGAKDISTKAISYAEKNVQASLDHAEKLLHAKDLTEVMRLHSEYVQAQMR
                     ALAEQASEMGQMVSRAAMDAAKPKS"
     gene            847..4143
                     /locus_tag="VN968_22770"
     CDS             847..4143
                     /locus_tag="VN968_22770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXL32091.1"
                     /translation="MSNAEFQLRGISDPRLAVHATSAIPAWLWSIDGTQVLWANAVGA
                     KIFGAGSGAALTKKLFGPADAHRRQVARLARTLPLNGAARLERLRGFGAPPGMLVTCG
                     CARLDFADGSQGVLVTAAENISRSMPLPERLQRLVDCIDAPIAAFSCDGTLVGASEAA
                     RPLLFFRNLSEAGLDDARSSALIQGRVETPIGSHHMVLQRVGHGADIGLVALITPAAD
                     QPIPDYELPATSGEAPAEFALFDEFAELTTSEITVEQAASEPAEPDPQTVSQFDGLPI
                     EASHGEAPPQTTTPDPEVYQDEPSPYIEAVADEPATPPTAEPSPAIDSAPPSPADAPS
                     WLNEPSPDPRQQPLRFTWRMDAGGRFALDPGEFTHLIGPRTAAGFDRPWSEIAETFGL
                     DPDGRVLRAVATSDTWTGITLSWPVDGGGRLAVELSGLPIYDRARNLAGYRGFGVCRD
                     LEALTRLAALQRFEFFSDPPAPPAAGLADEPDVASSGTAELPASIASETSQPTDLETS
                     VETPKNVLPFRSPGETKSPTLTPVENSAFNELARQLSERLGSENGGAAATTATSDAPE
                     ATAEQPAAPEVPEQSGAAPEWLAPPEPPARGEGRRDKALLDLLPVGVLIYRLDRLLYA
                     NAAFLERMGYASLHALEEAGGLDALYVEPGVSSASSTSDTGTPVTISASQFSAAEAPA
                     GIDAHLFTISWDDDSALALIFSGTGTQSANAAAAIAQAIPASPPPAVGHADEFGAILD
                     TTAEGIVMFDAAGSINFCNRSAEALFGYDGTEFVRRNFTELFAPESQRVVKDYLESIR
                     GSGAASLPGHGRDVLGRVSRGGLIPLSMTMGRTRAGVSNFFAVFRDLSQVGKSESELR
                     EARRLADRAASAKADMLARISHEVRTPLNAIIGFAEVMIDERFGALGNERYVEYMKDI
                     RASGERVIAIIDDLLDLSRIETGKLDLAFASQSLNDTVENCVAVMQPQANRERIIIRT
                     SLAHALPPVVADARALRQITLNLIGNSMHLANAGGQVIVSTALTDFGEVILRIRDSGQ
                     GLNDNEVAAAMEPFRTQAPSDQASNSSGVILSLTKALVEANRGQFHIKRGPHSGTLIE
                     VVFLRAMARA"
     gene            4298..>4452
                     /locus_tag="VN968_22775"
     CDS             4298..>4452
                     /locus_tag="VN968_22775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXL32092.1"
                     /translation="MRCLVVADLHYSLPQFDWLLSAAPHFDLVIFAGDALDVGSFVDF
                     RAQIVVVR"
BASE COUNT          736 a         1489 c         1407 g          820 t
ORIGIN      
        1 catccctccg gccgcaaacc cggcgaagcc gctttctggt tcgggcgata cccctccctc
       61 tttggtggag gggtttgaga ggcctcagct tttcggcttg gcggcatcca tcgccgcgcg
      121 gctgaccatc tggcccatct cgctggcctg ctcggccagc gcgcgcatct gcgcctgcac
      181 gtactcgctg tgcagccgca tcacttcggt cagatccttg gcgtgcagca gcttttcggc
      241 atggtccagc gaggcctgca cgttcttttc ggcataggat atcgccttgg tgctgatgtc
      301 cttggcgccg gcgcgaacgg ttgcaccttg ctcctcgagc gatccggcgg tctgctgcgc
      361 gctggtcagg aatttgtcaa aggccttgcg ggcctgctcg aaactcgcct cggccatcga
      421 tcgcatgtcc tttggaattt cgaaacggtc acgcccttca tcgctcatgg taccactcct
      481 tttttcgttt tcgtttgcag ggccatctac aaccccgcct ccccgttcgc ccactttctg
      541 aagcctgtcg acgaaagacc gtcgccggtg tgacgcggcg ccgatcggtt cccctatcgt
      601 ggacctctgg tcgccctttg caacacaaca acgcgggagc cggcacgtgg gtccgaacga
      661 attcgcaaga gccaggcgag ttaaggttag ccgggctttg gaccgcttgc cgattatcag
      721 cccgtggacc tgccgttgcc cgcgggcaat actaacgttc cgtcaaggct gtcatttgtc
      781 tggccgccga tatctgtcgc cacccaagcg aacctcggaa tcggaccacc ctgtcatgtc
      841 actgcgatga gtaatgcgga atttcagttg cgaggcatca gcgatccgcg gctggcggtg
      901 catgcaacga gcgctatccc ggcatggctg tggtcgatcg acggcacgca ggtgctgtgg
      961 gccaatgcgg tcggcgccaa aatattcggc gcaggcagcg gcgcggcatt gaccaagaaa
     1021 ctgttcggcc ccgcggatgc gcaccggcgt caggtggcgc gactcgcaag gacgctgccg
     1081 ttgaacggcg cggcccggct ggaacggctg cgcgggttcg gcgcgccgcc cggcatgctc
     1141 gtgacctgcg gctgcgcgcg gcttgatttt gccgacggca gccagggtgt cctggtgacc
     1201 gccgccgaaa acatcagccg ctcgatgccg ctgccggagc ggttgcagcg cttggtcgac
     1261 tgcatcgatg cgccgatcgc cgccttctcc tgcgacggca cgctggtcgg cgccagcgag
     1321 gccgcgcgac cgctgctttt ttttcgaaat ctctccgagg ccggccttga cgatgcgcgc
     1381 agcagcgcgc tgattcaggg ccgcgtcgag acgccgatcg ggagccacca catggtgctg
     1441 caacgcgtgg gtcacggcgc ggatatcggc ctcgttgcgc tgatcacgcc ggcagcggat
     1501 cagccgatcc ctgactacga actgccggcg acatcgggcg aggcaccggc cgagttcgca
     1561 ttgttcgacg agttcgcgga attgaccact tcggagataa cggtcgaaca ggcggcctcg
     1621 gagccggccg agccagaccc gcagacggtg tcgcagttcg atgggcttcc catcgaagcc
     1681 tcacacggcg aagcgccgcc gcagacaacg acgcccgatc cggaagttta tcaggacgaa
     1741 ccttccccct atattgaagc cgtggccgac gaaccagcaa ctccgcccac tgccgagccg
     1801 tcaccggcga tcgattccgc cccgccctcg ccggccgacg cgccgtcatg gctcaacgag
     1861 ccttcgccgg atccgcgcca acagccgtta cgcttcacgt ggcggatgga tgccggggga
     1921 cgcttcgcgc tcgaccccgg tgaattcacc cacctgatcg gtccgcgcac cgccgccggc
     1981 ttcgaccggc cgtggagcga gatcgccgaa acgttcgggc tcgatcccga cggccgcgtc
     2041 ctcagagcgg tcgccacgag cgatacctgg accggcatca ccttgagctg gcccgtcgac
     2101 ggcggtggcc gcctggcagt ggaattgtcg gggcttccga tctacgatcg cgcccgcaat
     2161 cttgccggct atcgaggttt tggggtttgc cgcgatctcg aagcgctgac gcggctcgcc
     2221 gcgttgcaac gcttcgaatt cttcagcgac ccgccggcgc cgccggccgc cgggctggct
     2281 gacgagcccg atgttgcgtc gtccggtacc gctgaattac ccgcgtcgat tgcgtccgag
     2341 acttcacaac caaccgatct ggaaacatcc gtggaaaccc caaagaacgt cctgccgttt
     2401 cggtcgcccg gcgagacgaa atcgccgacc ctgacaccgg ttgaaaacag cgccttcaac
     2461 gaactggccc ggcaattgtc ggagcggctc ggcagcgaaa acggcggcgc ggccgcgacc
     2521 acggcgacct ccgacgcgcc cgaagccacg gccgaacagc cggccgcgcc ggaggttccc
     2581 gaacagtccg gcgcagcgcc cgaatggctg gcgcctccgg agccgccggc gcgtggcgag
     2641 ggccggcgcg acaaggcgtt gctcgatctg ctcccggtgg gcgtcctgat ctatcggctt
     2701 gaccggctgc tctatgccaa cgctgcgttt ctcgagcgga tgggctatgc gagtctgcac
     2761 gccctcgagg aggccggcgg tctcgacgcg ctctatgtcg agcccggcgt gtcgagcgcc
     2821 agcagcacat cggacaccgg caccccggtg acgatttccg ccagccagtt ttcagcagca
     2881 gaagcgccgg cggggatcga tgcgcacctg ttcacgattt catgggacga tgattccgcg
     2941 ctggccctga ttttctctgg caccggcact caaagcgcaa atgccgctgc cgcgatcgcg
     3001 caagcgatcc ccgcgtcgcc gccgcccgct gtcggccatg ccgacgaatt cggcgcgatc
     3061 ctcgatacca cggccgaagg catcgtgatg ttcgacgccg cgggcagcat caatttctgc
     3121 aaccgcagcg ccgaagcgct gttcggctat gacggcaccg agttcgtgcg acgcaatttc
     3181 acagagctgt tcgcgccgga aagccagcgc gtggtcaagg actatctgga gagcatccgg
     3241 ggcagtgggg ccgccagcct gcccggtcac ggccgtgacg tgctgggccg tgtgagccgg
     3301 ggcggcctga tcccgctgtc aatgacgatg gggcgcaccc gggccggggt ttcgaatttc
     3361 ttcgcggtgt ttcgcgactt gtcgcaggtc ggaaagagcg agagcgaatt gcgcgaggcg
     3421 cggcggctgg ccgatcgcgc ggcaagcgcc aaggccgaca tgctggcgcg gatcagccac
     3481 gaggtccgca cgccgctaaa cgccatcatc ggctttgccg aagtgatgat cgacgaaagg
     3541 ttcggcgcgc tcggcaacga gcgatatgtc gaatacatga aggacatccg cgcttccggc
     3601 gaacgcgtga tcgccatcat cgacgacctg ctcgatctga gccggatcga gaccggcaaa
     3661 ctcgacctcg ccttcgccag ccagagcctc aacgacaccg tcgagaattg cgtcgcggtg
     3721 atgcagccgc aggccaatcg cgaacgcatc atcatccgga cctcgctggc gcacgctctg
     3781 ccgccggtgg tcgccgacgc ccgcgccttg cgccagatca cgcttaacct gatcggcaac
     3841 tcgatgcatc tcgccaatgc cggcggccag gtcatcgttt ccaccgcgct gaccgatttc
     3901 ggcgaagtga tcttgcggat ccgcgatagc ggccaggggc tgaacgacaa cgaagtcgca
     3961 gccgcgatgg agccgttccg aacgcaggct ccgtcggatc aggcctccaa cagctccggc
     4021 gtcattctgt cgctgaccaa ggcgttggtc gaagccaacc gcgggcaatt ccatatcaag
     4081 agggggccgc attcgggcac gctgatcgag gtggtgtttt tgcgtgcgat ggcgcgggcc
     4141 tgagatcgcc ggcaacgcgg cggccgttgc ggcaaagtgc cgcgtcgcgc aaacctgcgg
     4201 catgcttctt gctcaattct gatttctcag agcgccatga tttcttcaag gcgccgttcg
     4261 cgtcgcctgt ttcttgggca ttcgggttga tttgatcatg cgctgcctcg tcgttgcaga
     4321 cttgcactat tcattgccgc aattcgactg gctgctcagc gcggcgccgc atttcgacct
     4381 ggtcatcttc gccggagatg cgctcgacgt cggatcgttc gtcgatttcc gcgcccagat
     4441 cgtcgtggtg cg
//
LOCUS       DATNOI010000565         4133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1340469,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000565 DATNOI010000000
VERSION     DATNOI010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340469"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="VN968_22780"
     CDS             complement(<1..119)
                     /locus_tag="VN968_22780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXL32093.1"
                     /translation="MGYKVALVGATGNVGREMLNILDERKFPADEVVALASRR"
     gene            234..1040
                     /locus_tag="VN968_22785"
     CDS             234..1040
                     /locus_tag="VN968_22785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32094.1"
                     /translation="MQSFLGRCATLYNPTQTHPVLNDLADECCGMLARLVAYVGTLAL
                     LAIVGVHLWDQLPARKAAEASAKVGWSVATRSHPAFAISQFDLAEKTETYEILRHPGG
                     GRKDILRWAAADGKQDQKPVAELEIYRRGDESSESGPLADIAARMDPGGLRELEAAGV
                     IDSKFGAVTLLRVAGQPGGARSCLGFVKRLDEPNLQISGWTCQGVSLPARRTAIGCIL
                     SRLILLTAGNDPKLAELFAHAELKRGSCTASAASADWVTGAENPRLRGTF"
     gene            1208..1591
                     /locus_tag="VN968_22790"
     CDS             1208..1591
                     /locus_tag="VN968_22790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32095.1"
                     /translation="MTWKFPAANKLAVSLAAISVIAVGFAMSAANAEPKRQTDERGRP
                     YYGANGPNVSYQQGRTRIYVSRRSWLDAGVEVLPGDRKFTDYAFPPAIGYPSFARENN
                     NRPIDRQPLNPPSDLGGEPTKFPLY"
     gene            complement(1766..2878)
                     /gene="leuB"
                     /locus_tag="VN968_22795"
     CDS             complement(1766..2878)
                     /gene="leuB"
                     /locus_tag="VN968_22795"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HXL32096.1"
                     /translation="MATHKLLLLPGDGIGPEVMAEVKRLIGWLNAEGLAHFETEQGLV
                     GGSAYDADKVAISDATMALAQASDAVIFGAVGGPKWDSVPYEVRPEAGLLRLRKDMAL
                     FANLRPAICYPALAEASSLKREVVEGLDIMIVRELTGGVYFGEPKTISDLGNGQKRAV
                     DTQVYDTYEIERIARVAFDLARKRRNKLTSMEKRNVMKSGVLWNEVVTQVHAREYKDV
                     QLEHQLADSGGMNLVKWPKQFDVIVTDNLFGDMLSDIAAMLTGSLGMLPSASLGEIDV
                     KTKKRKSLFEPVHGSAPDIAGKGLANPIAMIASFAMALRYSFDMGALADKVEQAIAAV
                     LANGLRTADIKSEGTTAVSTTEMGEAILKELQALHG"
     gene            3111..>4133
                     /locus_tag="VN968_22800"
     CDS             3111..>4133
                     /locus_tag="VN968_22800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HXL32097.1"
                     /translation="MHAPNCPTSYAHPARLILILSLAPTVCLGIGRFAYSLVLPDMRD
                     QLGWSYSAAGFMNTINAAGYLAGALVASKLIRRFGLSAAVRGGTMACLVSLALCALSG
                     NFIVLSFARLLAGVGAAAAFVGGAALAATIAQSQGSRANFLLSLFYAGPGLGILSSGL
                     IAPFVLQAFGPGSWWIVWWAMTLLSVLMSVPLLLAPIDTKAGIADAAAPTRFAIGPVM
                     IYLAGYFLFGAGYIAYMTFMIAYVRDTGGGAAAQSAFWCLIGLSAFATPWVWRGVLAL
                     DRGGVSTTIILAVNAVGASLPLFGHSMAWLAASALVFGVSFFAIVGSTTAFVRFNYPP
                     SAWPTAI"
BASE COUNT          675 a         1353 c         1273 g          832 t
ORIGIN      
        1 ctgcggcgtg acgccagcgc caccacctcg tcagccggga atttgcgttc gtccagaatg
       61 ttgagcattt cccgcccgac attgccggtc gctccgacca gcgcgacttt gtaacccatc
      121 gttcactctc cgaagaaaaa tcctcaaccc ccgcaagctg cggaaaggga agaggcagcg
      181 cttctatgcg agaaacgccc ttaagacaac gtttgtgtgc attcgggcat ttgatgcaat
      241 cgtttttggg ccgctgtgcc actctttaca atccgaccca gactcatcca gtcttgaacg
      301 accttgccga cgaatgctgc ggcatgctgg cgcgtctcgt ggcctatgtg gggactctgg
      361 ccctgcttgc catcgtcggc gtccatttgt gggaccaatt gccggcccgc aaagccgctg
      421 aagcttccgc caaagtgggc tggagcgtgg ccacccgctc gcacccggcg tttgccatca
      481 gccagttcga tttggccgaa aaaacagaga cttacgagat cctccggcac cccgggggcg
      541 gccgcaagga catcctgcgc tgggccgccg cggacggaaa gcaagaccaa aagccggtcg
      601 ccgagctcga gatctatcgc cgcggcgacg aatcgagcga atcggggccg ctggccgaca
      661 tcgccgcccg aatggacccc ggcggcctgc gcgagctgga ggcggctgga gtcatcgaca
      721 gcaaattcgg cgcggtgacg ctgcttcgcg tcgcaggcca gccgggcggc gcgcgatcct
      781 gcctcggatt cgtcaaacgt ctcgacgaac ccaatctgca gatctccggc tggacctgcc
      841 agggcgtcag cctgccggcc cgccggaccg cgattggctg tattctgagc aggctgattc
      901 tgctcacggc cggaaacgat ccaaaactgg ctgaattatt tgcccacgcc gagctcaagc
      961 ggggcagctg caccgcgtca gccgcatcgg cggattgggt gacaggcgcg gaaaacccca
     1021 ggctgcgtgg cactttctga ccgggctggc cgaggcggcc agccaagatg ctggtttcca
     1081 tgcaagtttt tcgtattccg acgcattatt gtgatgcggt gtggcccgat tgaccttgtg
     1141 gccgcatacc cccggcagta aaaatgattg aaatcgatat ggtggcccgg ccgcccgtga
     1201 ggacctgatg acctggaaat tccctgctgc caataagctt gcggtgtcgc tggccgccat
     1261 ctccgttatc gctgttggct ttgccatgtc agcggccaac gccgagccca agcgtcaaac
     1321 cgatgagcgc ggccgcccct attacggggc gaacggtccc aatgtgtcct accagcaggg
     1381 acgcacccgc atctacgtca gcaggcgctc ctggcttgat gccggcgttg aagtgctgcc
     1441 cggcgaccgc aaattcaccg attacgcatt cccgccagcg attggctacc catcgttcgc
     1501 ccgcgagaac aacaaccgcc cgatcgaccg ccagccgctc aatccgccgt cggatttggg
     1561 cggcgagccg accaagttcc cgctctattg attttgacga gcacccgcga tccgtcgtcc
     1621 cggcgaacgc cgggacccat aatcgcttgc gtgtgtggtt aaagcgcgtg tgtggttaaa
     1681 gaaggcatct gctccagcgg cctaatcgcg gacacggagc atggatcccg gcgtcgccgg
     1741 gatgacgcgg ggtggcctgc cccgcctacc cgtggagcgc ctgcaactcc ttcaagatcg
     1801 cctcgcccat ctctgtcgta gagaccgccg tggtgccctc ggatttgata tccgcggtgc
     1861 gcaggccgtt cgcgagcacc gccgcgatcg cctgctcgac cttgtcggcc agcgccccca
     1921 tgtcgaacga atagcgcagc gccattgcga acgacgcgat catcgcgatc ggattggcga
     1981 ggcctttgcc ggcgatgtcc ggcgccgaac cgtgcaccgg ctcgaacagc gatttacgct
     2041 ttttggtctt gacgtcgatc tcgccaagcg aggccgacgg cagcatgccg agcgagccgg
     2101 tcagcatcgc cgcgatatcg ctgagcatgt cgccgaacag attgtcggtg acgatgacgt
     2161 cgaattgctt cggccatttc accagattca tgccgccgga atcggcgagc tgatgctcga
     2221 gctgcacgtc cttgtattcg cgcgcatgca cctgggtgac cacctcgttc cacagcacgc
     2281 ccgatttcat gacgttgcgc ttttccatcg aggtcagctt gttgcgacgc ttgcgcgcca
     2341 gatcgaacgc gacccgcgcg atgcgctcga tctcataggt gtcgtagacc tgggtatcga
     2401 cggcgcgctt ctggccgttg ccgagatcgg agatggtttt cggctcaccg aaatacactc
     2461 cgccggtgag ttcgcgcacg atcatgatgt cgagcccctc gaccacctcg cgcttcaggc
     2521 tcgaggcttc cgcgagcgcg ggatagcaga tcgcgggacg aaggttggcg aacagcgcca
     2581 tgtccttgcg cagccgcaac aggccggctt cgggccgcac ctcatagggg acgctgtccc
     2641 acttcggacc gccgactgcc ccgaaaatca ccgcgtcgct ggcttgcgcc agcgccatgg
     2701 tggcgtctga tatcgcgacc ttgtcggcgt cataggcgct gccgccgacc aacccctgct
     2761 cggtctcgaa atgcgccagg ccttctgcgt tgagccagcc gatcaggcgc ttcacctccg
     2821 ccatcacttc ggggccgatg ccatcgccgg ggagaagcag cagtttgtgg gtcgccatgg
     2881 ttcgttacct caggagtatt gtttgtccgt cattgccgga cttgatccgg caatccagct
     2941 tttttctgaa gacgatggat gcccgggtca ggcccgggca ttacgacgga taaggaaatc
     3001 cgcgcgcgga gtgctagagc ggcattgcgc aattggcaag acaccattgc ggccggcggc
     3061 ctgtgcaatg gccctggacc ctgccacatt gcgccctgcc ggagtatccc ttgcacgcac
     3121 ccaactgccc cacgtcctat gcgcatcccg cgcggctgat ccttattctg tcgctggcgc
     3181 ccaccgtctg cctcggcatc ggccgcttcg cctattcgct ggtgctgccg gacatgcgcg
     3241 accagctggg ctggtcctat tccgccgcgg gtttcatgaa caccatcaac gcggcggggt
     3301 atctcgccgg cgcgctggtc gcatcgaaac tcatcaggcg ctttggacta tccgccgcgg
     3361 tgcgcggggg aacgatggcc tgcctggtgt cgctggcgct gtgtgcgctg tcaggcaatt
     3421 ttattgtttt gagttttgcg cggctgctcg cgggtgtcgg cgcggcggcg gcattcgtcg
     3481 gcggcgccgc gctggcggcg acgatcgcgc aatcgcaggg ctcgcgggcg aattttctcc
     3541 tgagcctgtt ctatgccgga cccgggctcg gcatcctgtc gtccggcctg atcgcgccgt
     3601 tcgtgctgca ggcgttcggg ccgggatcgt ggtggatcgt ctggtgggcg atgacgctgc
     3661 tttctgttct gatgagcgtt ccgctgctgc tggcgccgat cgataccaaa gccggcatcg
     3721 ccgatgcggc ggcgccgacc aggttcgcga ttgggccggt catgatttat ctcgccggct
     3781 atttcctgtt cggcgccgga tacatcgcct acatgacctt catgatcgcc tatgtgcgcg
     3841 ataccggcgg cggtgccgcg gcgcagagcg cgttctggtg cctgatcggc ctgagcgcgt
     3901 tcgcaacacc ttgggtctgg cgcggtgtgc tggcgcttga ccgtggcggc gtctcaacca
     3961 cgatcatcct cgccgtcaac gcggtcggcg caagcttgcc gctgttcgga cactccatgg
     4021 cgtggctcgc cgcatcggcg ctggtgttcg gtgtctcgtt cttcgccatt gtgggctcga
     4081 ccaccgcctt cgtgcgcttc aactacccgc cgtccgcctg gcccaccgcg att
//
LOCUS       DATNOI010000566         2995 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2323978,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000566 DATNOI010000000
VERSION     DATNOI010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323978"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="VN968_22805"
     CDS             <1..1279
                     /locus_tag="VN968_22805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HXL32098.1"
                     /translation="AADKALMKQLSGYPQALGMSAGVMLVLALLPGIPMIPFLLLGSG
                     AAALALSARKRNRAASEAQAIEAAAPAAAAAASAAGEEPIATALKIDDLKIELGYALL
                     PLVNGPDGTDRLTEQIKALRRSLAVEMGFVMPAVRILDNVQLEANTYVIKIKEVDAGS
                     GKIWPNQFMVMDPAGNQVGVPGIHTIEPTFGLPATWVDASLKEEATLKGYTVVDAATV
                     LSTHLTELLKNNMSDLLSYGEVQKLLKDLPKEQGELVKDIVPSQVTMSGIQRVLQLLL
                     AERISIRDLSTILEGIADALAFSRNPSTMVEHVRARLARQICAQNTSHNGYLPLIALS
                     AKWEQAFAESLIGQGEERSLAMQPSKLSEFMTSVRDRFEQAAREGEAPVLVTSAAIRP
                     FVRSLVERFRSQTTVLSQAEIHPRARLKTVGSV"
     gene            1596..1796
                     /locus_tag="VN968_22810"
     CDS             1596..1796
                     /locus_tag="VN968_22810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32099.1"
                     /translation="MNHSIYSADRTTHIKIVVVALVAGIAVAGFGISARTNSDEGYSQ
                     TARVMKAGKAVTITSSDTSTVR"
     gene            complement(1975..2847)
                     /locus_tag="VN968_22815"
     CDS             complement(1975..2847)
                     /locus_tag="VN968_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HXL32100.1"
                     /translation="MPYSEDHIALAAEYALGTLDADERAQVETMMSVDKDFAAIVDAW
                     EDKLSVLNQMVGSVEPRAEVWDKIKEAVGHSDPQVPLVLPQAPPVVVPEVKEPAEVVD
                     TSNVIRLAAQARRWRNVASFTTAIAAALVAMIAVGVYQPDLLPDGVRPKPRVQVVEVR
                     TPAVSATPSAQYVAVLQKDGGSPAFILTVDGATKNFTVRKVGAAPEPGKSFELWLISD
                     KLQRPRSLGVIGGGDFTARPVLSSYDNDTINTATYAVTVEQAGGSPDGNPHSAPVFTG
                     KLIESVPPAPSPAR"
     gene            complement(2851..>2995)
                     /locus_tag="VN968_22820"
     CDS             complement(2851..>2995)
                     /locus_tag="VN968_22820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HXL32101.1"
                     /translation="DRQKLVLLAYYNGWSREQLAVKFETPVNTVKTWLRRSMLDIREC
                     LGL"
BASE COUNT          598 a          944 c          898 g          555 t
ORIGIN      
        1 cgccgccgac aaggcgctga tgaagcagct ttccggctat ccgcaggcgc tcggcatgtc
       61 ggccggcgtg atgctggtgc tggcgctgct gccgggcatt ccgatgatcc ctttcctctt
      121 gctgggcagc ggtgcggcgg cgctggcgtt gtcggcacgc aaacgcaatc gggctgcctc
      181 ggaggctcaa gccatcgagg cggcggctcc agccgcggcc gcggcggcaa gtgctgcggg
      241 ggaagagccg attgcaaccg cgctgaagat cgacgatctc aagatcgaac tcggctatgc
      301 gttgctgccg ctggtgaacg gcccagacgg caccgaccgc ctgaccgagc agatcaaggc
      361 gctgcgccgc tcgctcgcgg tcgagatggg ctttgtgatg ccggcggtgc gcattctcga
      421 caatgtgcag ctggaagcca acacctacgt catcaagatc aaggaagtag atgcaggctc
      481 gggcaagata tggccgaacc agttcatggt gatggacccc gccggaaatc aagtcggggt
      541 acccggcatt cacaccatcg agccgacctt cggactgccg gctacctggg tcgacgcctc
      601 cttgaaggag gaagccacac tgaagggtta tacggtggtc gatgccgcga ccgtgctctc
      661 gacccacctc accgaactgc tcaagaacaa catgtcggac ctgctgtcct atggcgaggt
      721 gcaaaaactc ctgaaggatc tgccgaagga gcagggcgag ctggtcaagg acatcgtgcc
      781 gagccaggtc acgatgtcgg gcatccagcg cgtgctgcaa ctgttgctgg ccgagcgtat
      841 ctcgatccgc gatctgtcga ccattctcga gggcattgcc gacgcactgg ctttttcgcg
      901 caacccctcg acgatggtcg aacacgttcg cgcgcggctg gcgcgccaga tctgcgcgca
      961 gaatacatcc cacaacggct acctgccgct gatcgcgctg tcggcaaaat gggagcaggc
     1021 cttcgccgaa tcgctgatcg gccagggcga agaacgcagc ctcgccatgc agccttccaa
     1081 gctgtccgag ttcatgacct cggtgcgtga ccgcttcgaa caggccgccc gcgaaggcga
     1141 ggccccggta ctggtcacct cggcggcaat ccggcccttc gtccgctcgc tggtggagcg
     1201 attcagatcg caaaccacgg ttctgtcgca ggcggaaatt catccgcgcg cgcggctgaa
     1261 gacggtcgga agcgtctgac ggcgggcaaa atcgaagcgc tgagagcggg actttttcgc
     1321 cacaggcaaa cgatttatcg acccgccatg attcccgccc ttttgcggca caagatgggc
     1381 gaaaatgtta aacgactgta ataactgaat tatttttcat ttcgaatgtt ctaacggcca
     1441 agccaatcgc ggcggatcaa gtcttatcac tgccttgtga tcgcctcttt ggaaccaaac
     1501 gccgaattcc tacgtttcct gccatgagat gttgaacctg ccggcggacg aaaccgacca
     1561 attcgcaaga ggcagtacga cggcaggaga cttcgatgaa ccactcgatc tacagcgcgg
     1621 atcgcacgac gcacatcaag atcgtcgtcg tcgcactcgt tgctggtatc gcggtggcgg
     1681 gcttcggcat ctcggcacgc accaattcgg acgagggata tagccagacc gcgcgcgtca
     1741 tgaaggccgg caaggccgtg acgatcacga gttcagacac atccacggtc cgctaacaaa
     1801 ggatttccgg aattcgcgcg gcctctttag cagcccccca aagtcgccac gtggatatgt
     1861 agaagaccca aactacccca agtcgactac cgaaaacgcc cgctccccac gggcgttttc
     1921 tctttgtgcg cctgcgactt cggcacgcgc aaatctccag acatgaatca gattctatcg
     1981 cgcaggtgaa ggcgctggcg gcacgctctc gatcagcttg ccggtaaaga cgggcgccga
     2041 gtgcgggttg ccatcaggcg atccgccggc ctgctcgacc gtcaccgcat aggttgcggt
     2101 gttgatggtg tcgttatcgt aagacgaaag caccggacgc gcggtgaagt cgccgccacc
     2161 aatcacgccc agcgagcgcg gtcgttgcag tttgtcggag atcagccaga gctcgaagct
     2221 cttgccgggc tcgggggccg cgcccacctt gcgaacggta aaattcttgg ttgccccgtc
     2281 gaccgtgaga atgaacgcgg gcgatccgcc gtccttctgc aacacggcga cgtattgcgc
     2341 ggacggcgtc gccgatacgg cgggcgtcct gacttcgacc acctgcacgc gcggtttggg
     2401 acgaacgcca tcgggaagta aatccggctg atagactcca accgcgatca tcgcgaccag
     2461 tgctgccgcg atcgccgtgg tgaagctcgc aacattgcgc cagcgccggg cctgggcggc
     2521 caatcggatg acgttggagg tgtccaccac ctcggcaggc tccttgacct caggcacaac
     2581 aaccggcggc gcctggggaa gcaccagcgg cacctgcggg tcggagtgtc ctaccgcttc
     2641 tttgatcttg tcccagacct cagcgcgcgg ctcgaccgat ccgaccatct ggttcaagac
     2701 actgagcttg tcctcccacg catcgacaat cgccgcgaag tccttgtcga cggacatcat
     2761 ggtctcgacc tgcgcccgct catccgcatc gagggtgccg agtgcatatt ccgcggcgag
     2821 cgcgatatgg tcttcgctat agggcatcat ctagagtcca agacactccc ggatatccaa
     2881 catgctgcgg cgcagccagg tcttcacggt attcaccggc gtctcgaact ttaccgccag
     2941 ttgctcgcga ctccagccgt tgtaataggc cagcagcacg agcttttgcc gatcc
//
LOCUS       DATNOI010000567         4591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_50339,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000567 DATNOI010000000
VERSION     DATNOI010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50339"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="VN968_22825"
     CDS             complement(<1..1520)
                     /locus_tag="VN968_22825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HXL32102.1"
                     /translation="MPELTTVTSFGRVISVRGSLARVGLLAASQLAIPEVRATVGRFV
                     SIRCANSTIVAMITEVSCEDLPVSDVYIASASVDLLGEILGGPERPKFQRGVTHYPTI
                     SDAVELITNQELRTVYAPSGSDQIDVGTLQQDPSVIAYVDVEEMLSKHFAVLGSTGVG
                     KSSGVSLLLNEILKARPTLRVFLLDVHNEYGRCFGDRALVLNPRNLKLPFWLFNFEEI
                     VDVLFGGRPGVPEELDILAEIIPMAKGIYTQYQNADRIGLKRLDPKAGYTVDTPVPYR
                     LVDLISLIDERMGKLENRSSRIIYHKLISRIETVRNDPRYAFMFDNANVGGDTMAEVV
                     SHLFRLPANGRPMTIMQLAGFPAEVVDSVVSVLCRMAFDFGLWSDGVSPMLFVCEEAH
                     RYASADRSIGFGPTRKAVSRIAKEGRKYGVYLGLVTQRPAELDATIISQCNTLFAMRL
                     ANERDQTLLRSAVSDAAANLLSFVPSLGTREVLAFGEGVALPTRLRFKEVPVHQLPR"
     gene            1879..3138
                     /locus_tag="VN968_22830"
     CDS             1879..3138
                     /locus_tag="VN968_22830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL32103.1"
                     /translation="MSELARPSALAPFRYRNYRFQWPADLLTSWAFEMETLILGWYVL
                     VETGSVLLLTLFASLSYVGTLIAPLFGVVGDRIGHRDLLAMMRATYALLAGTLMTLAL
                     TGHLSALYVLVIVAVMGLVRPSDLGVRGALVATIMPSGQLIGAISLSRTTMDTARIAG
                     ALSGAGLFAALGMGPAYMAIVRLYVVSTLLTLCVAAPSKPHPIDEGAGGTLQHSPMRD
                     LKQGMAYVWTTPRMRAAMWIAFLANLTAYPLSNGLLPYVAREIYRTNQTGLGYLSASF
                     AVGSLVGSVLLSLIGGIRVARLLIGATVMWYAALLLFVQMQTVPAAIACLMLAGFSQS
                     LCMISIAVILMRTSSAHFRGRVMGVRMLVIYGLPLGLLAAGSLIDEIGFAATGTLYAT
                     AGVALMMAIVLHWRADLWHAHAPANAK"
     gene            3242..3955
                     /locus_tag="VN968_22835"
     CDS             3242..3955
                     /locus_tag="VN968_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HXL32104.1"
                     /translation="MVRTSASSATATRKPNLREAILAAAEELFATNGFNAVSIRDIAQ
                     AAGANPGSVTYHFKTKDGLLLEIYRRHCGPMNRRRSELLAAARRVRDQQDRLEAIVRA
                     YVLPAFSSGSDLAGGGARFTRLRAVMSAEGNEVARRIIAQTFDDTSHAFIDAIHESLP
                     HIPRTEIVWRSHFLLGALYYTLVTPERVSRLSRGSADGADAGNAIEQLVRATIASFQA
                     PPLDQPAPSRRRSAAQNMT"
     gene            4118..>4591
                     /locus_tag="VN968_22840"
     CDS             4118..>4591
                     /locus_tag="VN968_22840"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HXL32105.1"
                     /translation="MSKTGLVVSAHPGDFVWRAGGAIALHARKGYRVKIVCLSYGERG
                     ESQFAWKEQGASLASVKAGRKDEAERAAAALGAEIEFFDCGDYPLKLNETHFDRMVDI
                     YRELNPSFVLTHALEDPYNFDHPDAAHFAQQTRVVAQAMGHKPDAKYQYSAPPVFL"
BASE COUNT          848 a         1373 c         1459 g          911 t
ORIGIN      
        1 cgcggcaatt gatgcaccgg cacttccttg aagcgcagcc gggtcggaag tgcgacgcct
       61 tcaccgaagg ccaggacttc gcgggtaccg agcgagggga cgaacgacag caggtttgcc
      121 gcggcatccg aaaccgccga gcgcagcagc gtctggtcgc gttcgttggc aagccgcatc
      181 gcaaacagcg tgttgcactg ggaaatgatg gtggcatcga gttcggcggg acgctgggtg
      241 acgaggccga gatagacgcc gtatttgcgg ccttccttgg cgatacgcga tacggctttg
      301 cgtgtcggcc caaaaccgat gctgcgatcg gccgaggcgt agcgatgggc ctcttcgcag
      361 acgaacagca tcggcgaaac gccgtcgctc cacagcccga agtcgaacgc catccggcac
      421 agcaccgaca ccacggaatc gacgacttcg gcgggaaagc cggcaagctg catgatggtc
      481 atcggcctgc cgttggcggg aagccggaac agatgactga cgacttccgc catggtgtcg
      541 ccgccgacat tggcgttatc gaacatgaat gcgtagcgcg gatcgttgcg cacggtctcg
      601 atccgcgaaa tcagcttgtg atagatgata cgcgatgagc ggttctcgag cttgcccatg
      661 cgctcgtcga tcagcgaaat cagatcgacc aggcgatagg gcaccggcgt atcgacggtg
      721 tagccagctt tgggatcgag gcgcttcaac ccaatccgat cggcgttctg gtactgggta
      781 tagattccct tcgccatcgg aatgatttcg gcgagaatat cgagttcctc gggcacgccg
      841 ggccggccgc cgaacagcac atcgacgatc tcttcgaaat tgaacaacca gaacggcagc
      901 ttcagattgc gcggattgag aacaagggcg cggtcgccga aacagcggcc atattcgttg
      961 tgaacatcga gcaggaagac ccgtaaagtc ggccgtgcct tcaaaatctc attcagcaac
     1021 agcgatacgc cgctggattt gccgacgccg gtagagccaa gcaccgcgaa gtgcttggag
     1081 agcatctcct cgacatcgac ataggcaatg acggagggat cctgctgcag ggtgccgaca
     1141 tcgatctggt ccgatccgga cggcgcataa accgttcgaa gctcctgatt ggtgatcaac
     1201 tcgacggcat cgctgatcgt gggataatgg gtgacaccgc gctggaattt ggggcgctcc
     1261 ggtccgccca ggatttcgcc gagcagatcg accgaggcgc tggcaatata aacgtcggag
     1321 accggcagat cctcgcagga tacttccgtg atcatggcga cgatggtgga atttgcgcag
     1381 cggatgctga cgaaccggcc gacggtggcc ctcacttcgg ggatggccag ctggctcgcg
     1441 gccagcagtc cgactcgcgc gagggatcca cgcaccgaaa tcacgcgtcc aaaggatgtc
     1501 acagtggtca attccggcat gtcatgaatt cagatctgtg cacgactatg cagctggtgg
     1561 ttggacaaac ggttaagccc gcgaacctcc gggttggtta aatcggcggt atcttggtca
     1621 ggatatggtt ttgccgggcc gctctggcgt gccttcatcg tcggatttgc tggaaaatcg
     1681 acgatttgag ctccgcccgt cagcgcccgc ggcaggtccg gcttaacgtc gggtttacca
     1741 cccgcggagg agacctgctt aacgcgccgg cttttttgcg ggcacggccg ggggctggcc
     1801 tcggccgccg cgacgcggta gattttgccc caataagaaa cgggcagggt ggaacgcgat
     1861 gaaacgcggc atcgagtttt gagcgagttg gcgcggcctt ccgcgctcgc ccccttccgt
     1921 tacaggaatt atcgttttca gtggcccgcc gacctgctca catcctgggc gttcgagatg
     1981 gagacgctga ttctcggctg gtacgtgctg gtcgagaccg gatcggtgct gctgctgacc
     2041 ttgtttgcct cgcttagcta cgtcggcacg ctgattgccc cgttgttcgg cgtggtcggc
     2101 gatcgcatcg gtcaccgcga cctgctcgcc atgatgcgcg cgacctatgc gctgctggcg
     2161 ggcacactga tgacgctggc gctgacgggg catctgagcg cgctctatgt ccttgtcatc
     2221 gtggctgtca tgggcctcgt tcgaccgtcg gacctcggcg tgcgcggtgc gctggtcgcg
     2281 accatcatgc cgtcaggtca attgatcggc gccatcagcc tttccaggac cacgatggat
     2341 accgcgcgca tcgccggtgc gctgagcggg gccggattgt tcgcagccct cggcatgggc
     2401 ccggcctata tggcgatcgt ccgcctctac gtcgtcagca cgctgctgac cctctgtgtc
     2461 gcggcgccgt cgaaaccgca tccgatcgat gaaggggccg gcggcacgct tcagcactca
     2521 ccgatgcgcg acctgaagca gggcatggcc tatgtctgga ccacgccgcg gatgcgcgcg
     2581 gcgatgtgga tcgcgttcct cgccaatctg accgcctatc ccttgtcgaa cggattgttg
     2641 ccctatgtcg cgcgcgagat ctaccgcacc aaccagaccg ggctcggcta tctgtcggcc
     2701 agcttcgcgg tcggctcatt ggtcggttct gtactcctga gcctgatcgg cggcattcgc
     2761 gtcgcgcggc ttttgatcgg cgcgacggtg atgtggtacg cggcgctgtt gctgtttgtg
     2821 cagatgcaaa cggtgcctgc cgcgatcgcg tgcctgatgc tggcgggctt ctcgcaaagc
     2881 ctttgcatga tctcgatcgc ggtcatcctg atgcgcacct cgagcgcgca tttccgcggc
     2941 cgggtgatgg gcgtgcgcat gctggtgatc tatggactgc cgcttggcct gttggcggcc
     3001 ggcagcctga tcgacgagat cgggtttgcc gcgaccggca ccctctacgc gacggccggc
     3061 gtggcgttga tgatggcgat cgtgctgcac tggcgcgcgg atctgtggca tgcccacgcg
     3121 ccggcgaatg cgaagtaggt agatttgcat tcgcggccgc gctccaggct gctcaggcat
     3181 cagtactaac gcccatggtt attgttgact agaccttatc ttttgtacga tagtacaaat
     3241 gatggtccgc acgtccgcca gcagcgccac tgcaacccgc aaaccgaatt tgcgggaggc
     3301 gatcctcgcc gccgcggaag agttgttcgc gaccaatggc ttcaacgccg tttcaattcg
     3361 ggatattgcg caagccgccg gcgccaatcc cggcagcgtg acctatcact tcaagacaaa
     3421 agacggcctg cttctggaaa tctaccgtcg tcattgcgga ccgatgaacc gccgacgctc
     3481 cgaattgctg gctgccgcca ggcgggttcg tgatcagcag gacaggctcg aggcgatcgt
     3541 tcgcgcctat gtgctgcccg cattctcttc aggcagcgat cttgccggcg gcggagcgcg
     3601 ttttacgcgg ctgcgcgcgg tcatgtcggc tgagggaaac gaagttgcgc gccggatcat
     3661 cgcgcagacc tttgacgata ccagtcatgc cttcatcgat gcgatccacg aaagtctgcc
     3721 gcacatcccg cgtaccgaaa tcgtctggcg cagccacttc ctgctcgggg cgctctacta
     3781 cacgctggta acgcctgaac gcgtgtctcg gctgtcgcga ggcagcgccg acggcgcgga
     3841 cgcgggaaac gcgatcgagc agctggtgcg ggcgacgatc gcttcgtttc aagcgccccc
     3901 gcttgatcag cccgcgccct cgcggcgacg atctgcagca caaaacatga cttaggcgag
     3961 ggctgacatg gcgcgctgcc gcttgcgggt ctgctgcaat cgttgaacca gggaacagga
     4021 cggatggatc tgataggaca tgtccggctc caggctcaca cgttcgctcg attttttgaa
     4081 gatatcggaa gatcaaagcc gacccgaaag gaatgcgatg tcaaagacag gtttggtggt
     4141 ttcggcgcac ccgggggatt ttgtgtggcg cgccggaggg gcgatcgccc tgcacgccag
     4201 gaagggctac cgcgtcaaga tcgtgtgcct gtcctatggc gagcgcggcg agagccagtt
     4261 cgcctggaag gaacaaggcg cgtcattggc ctccgtcaag gccgggcgca aggacgaggc
     4321 cgaacgcgcc gcagccgcac ttggcgccga aatcgagttc tttgattgcg gtgactatcc
     4381 attgaagctc aacgagaccc atttcgaccg gatggtcgat atctatcgcg agctcaaccc
     4441 cagttttgta ctcacccacg cgcttgagga tccctacaat ttcgatcatc ccgatgcggc
     4501 gcattttgcg cagcagacgc gggtggtggc gcaagcgatg ggtcacaagc cggatgcgaa
     4561 ataccagtat tccgcgccgc cggttttcct g
//
LOCUS       DATNOI010000568        19303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1165549,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000568 DATNOI010000000
VERSION     DATNOI010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 19303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19303
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165549"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VN968_22845"
     CDS             complement(<1..699)
                     /locus_tag="VN968_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5938 domain-containing protein"
                     /protein_id="HXL32106.1"
                     /translation="MSEKKPVIVYGVSGYTGRLVCEYLREYNIPFIAAGRDAAKVKAV
                     VEKIPGIETADYEVVGVEHTVEALTKLFRGAKVVSNMVGPFIKYGPEVVEACLAAGCH
                     YSDTTGEQDWVLLAQERWGEKFAAKGLLLAPNIAQMYTTGEIAANICLETPGLDTLDI
                     LVLWKGFPTYASTQTIFTILKANWYYLVQNKFVEWDVTANFEVNVPGQHEGAIAVPWG
                     GTAHPVWFKNDPRVS"
     gene            complement(741..1544)
                     /locus_tag="VN968_22850"
     CDS             complement(741..1544)
                     /locus_tag="VN968_22850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL32107.1"
                     /translation="MSEKSLAGRKAMVTGGARGIGAAIAEALTKAGASVMIGDILADL
                     GKETAGRLAKTGAKTGFVELNVTDDAEWEKAVAATIATLGGYDILINNAGIEITSLVV
                     DLRAEDLRRMCDVNVVGTALGMKHAFRAMRPGGGAGAGGTIVNIASVAATIAFPAIAG
                     YSGTKSAVDRMTRVAAMEAGKLGYGVRVNCLYPGLVPTDMGMQLANDIVAAGLAPDVG
                     AAVASVVEQTPLGRLGEVGDMADAAVFLCSNEARFITGAGLPVDGGMGM"
     gene            1983..2537
                     /locus_tag="VN968_22855"
     CDS             1983..2537
                     /locus_tag="VN968_22855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL32108.1"
                     /translation="MELAEAALETLAELGYARTSLREIAQKSEFTHGVLHYYFTDKVD
                     LICCCVRHYKAKCVTRYDQITSAAQSRDELMKGFLEKLGETVRDEAQMHRLWYDLRSQ
                     SLFEEAFRDDVAEIDKSLEDMVWRIASRFASLGGRQPAVSRGALYALFDGLFQRCLLR
                     HLSGDRKAIADLREEVARLLPTVA"
     gene            complement(2825..3595)
                     /locus_tag="VN968_22860"
     CDS             complement(2825..3595)
                     /locus_tag="VN968_22860"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXL32109.1"
                     /translation="MHRSKYLFHWLSSCLELLATGLAGDTVRAVPCGCQPAPFLTIVH
                     VAHDGLPDHSAAVRRQEFRCQCTSPNHLSVFDRDEYVIWGYSAHRDTLRIEGAADAIA
                     SEDARAAVLAQLADWNADLRYLVAHADASSLSSFVVKSSVPIPHWTTSRITLLGDALH
                     NMTPYRGIGANTALRDAAVLRDALRDVLNGRRDLLGALSAYEEEMIKYGFAAVRASLA
                     QMERLHTRSLTNRFATKVLFRILDLSPALRKRVLDLGG"
     gene            3690..3926
                     /locus_tag="VN968_22865"
     CDS             3690..3926
                     /locus_tag="VN968_22865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32110.1"
                     /translation="MKRFILALFALVTLSAAAVTTANAVEFGVGPNGVYVGPDRDRDY
                     HERYYNNYGSCRVVITHRINRFGEDVEVRKRICD"
     gene            complement(4122..4259)
                     /locus_tag="VN968_22870"
     CDS             complement(4122..4259)
                     /locus_tag="VN968_22870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32111.1"
                     /translation="MTGPDGKAEFGGCDRVRTRSGHVCENYVFCDHPFFARVGGEIAG
                     A"
     gene            complement(4726..5379)
                     /locus_tag="VN968_22875"
     CDS             complement(4726..5379)
                     /locus_tag="VN968_22875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32112.1"
                     /translation="MANFEQLTGDEFDDVIAGSSSSEAFIRRYMGFPPSGDDHCFVHR
                     GPLTTSGHFEAPGYCTLVVGDLTVDGLIDLNNPEGFDEGGLFIVIGNVTCRAFSGHYG
                     KSTFIDGNLNASELVLNAYEDSSLVVTGDLKTTLFHGRDIWAEVGGVADMECANGYCL
                     PIGYKNAAKQAIRARRSTTSARVLDPAVLDDEDDLKVDEALQRLKQGQSILRSKRNI"
     gene            complement(5366..5617)
                     /locus_tag="VN968_22880"
     CDS             complement(5366..5617)
                     /locus_tag="VN968_22880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32113.1"
                     /translation="MFLWCVFGLGLVIGYAAVYFDWMVYAQPFHVADGNGAKAVGIEF
                     ILAPAGAVVMGFFVTFRLALGAARRSVAKRGRRSDDGKF"
     gene            complement(6206..6282)
                     /locus_tag="VN968_22885"
     tRNA            complement(6206..6282)
                     /locus_tag="VN968_22885"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6246..6248),aa:Met,seq:cat)
     gene            6768..8372
                     /locus_tag="VN968_22890"
     CDS             6768..8372
                     /locus_tag="VN968_22890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXL32114.1"
                     /translation="MTDTFDFVVVGGGSGGCAVAGRLSEDPQTSVALLEAGGRGDNWV
                     VTTPGALALMVPSKLNNWAFDTVPQAGLQGRIGYQPRGKGLGGSSAINAMVYIRGHRS
                     DYDQWASLGNAGWSYADVLPYFKRSEDNADFDGEYHGKGGPLPVNKLRTDNPVQQTFL
                     QGAGEAQFRIREDFNAEDHEGLGVYQVTQRNGERWSAARAYVHPHMATRANLRVKTHA
                     QTTRILFEGKRAVGVEYRQGEATKQMRGRREVILSSGAFQTPQLLLLSGVGDGAALAR
                     HGIATTHHLPGVGQNLQDHPDFIFAYLSDSPYLTGMSFRGIAHQLRAIMQYRRERRGP
                     MTSNFAECGGFLKTSADLDIPDIQLHFGMAIVDDHGRKRRWGRGFSCHFCLLRPKSRG
                     SVALSSADPLAAPAIDPNFLGHVDDLESMVAGYKTTRRLMETPALRALQKKEMFTTGV
                     ETDDDIRALLRARVDTVYHPVGTARMGTDALAVVDPTLKVHGLEGLRIVDASVMPTLI
                     GGNTNAPTIMIGEKAADMIKAELWAG"
     gene            complement(8520..9281)
                     /locus_tag="VN968_22895"
     CDS             complement(8520..9281)
                     /locus_tag="VN968_22895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL32115.1"
                     /translation="MSGLFDVSKEIILVTGASQGLGRQFARVLSAHGAAVALAARQTA
                     KLKGLEEEILGQGGRAVAVQMDVTDIASIGKAIDSAEATLGPVSVLVNNAGIAIEKLA
                     IDQTEQDWDAVISANLKGAYFAATEMARRMIARKQEGNIVNIASVLGLGVMKFLSPYT
                     ISKAGIIQATKAMALELAGNRIRVNALAPGYIDTEMNHEFWSSPAGEKLTKRIPQRRV
                     GAEADLDGAIMLLASNASRYMTGSVVTVDGGFLLN"
     gene            9401..9913
                     /locus_tag="VN968_22900"
     CDS             9401..9913
                     /locus_tag="VN968_22900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HXL32116.1"
                     /translation="MNSFDAVVYLGLVIAVVTGFNAGLLRSAVTILAYLLAMPIAVWA
                     MSLIGSQIDGALGSTWAQNWLLFFGIFLIAGMVLGKLMRMALDETIGSEAGIADRLAG
                     ALLGAVRVGLVATTLVLIFDLVPSDRQPSFLVGSQLRPLLSAAGQKGFKSLPPDVTAY
                     IDRLKKDQRI"
     gene            10065..10850
                     /locus_tag="VN968_22905"
     CDS             10065..10850
                     /locus_tag="VN968_22905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL32117.1"
                     /translation="MDLGIKGRRALVCASSKGLGRACAMALAAEGVHVTLTARGAEAL
                     AKTAAEIRKASPGVTVTEVAGDITTPAGREAALKACPDPDILVNNAGGPPPGDFRNWT
                     RDDWIKAIDANMLTPIELIKATVDGMMARKFGRIVNITSAAVKAPIEVLGLSNGARAG
                     LTGFVAGIARKTVINNVTINGLLPGPFNTDRLRGPVAKIDADKRGITVDQLLAERAKL
                     NPAGRFGDPEEFGQACAFLCGAKAGFITGQNILLDGGAFPGTL"
     gene            10866..11855
                     /locus_tag="VN968_22910"
     CDS             10866..11855
                     /locus_tag="VN968_22910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HXL32118.1"
                     /translation="MKAVWYERTGAAPDVLTCGDMPTPEAGPGEVRVRLQASGVNPAD
                     VGRRAGSYRAMEFPRVIPNSDGAGIVDQLGAGVTRLSVGQRVWLFNGQRNGRAFGTAA
                     EYVALAEHLVTPLADNVSFAAGATLGIPGMTAWCGLFGDGPITGQTVLVTGGAGAVGH
                     YAVQLAKWGGARVIATVSSSFKAEQARLAGADLVIDYKTEDVIAKAMAFTEQRGVNRV
                     VDVDFGGNVATTLKLMAMNSTIAVYATNGNRSPVVPMRELMERCVALRALVLFALPQP
                     MLAAAQADISKWLAAAPRIHNIAAQFALSETAQAHLAVEKGDKLGTVVVDCAR"
     gene            complement(12135..12545)
                     /locus_tag="VN968_22915"
     CDS             complement(12135..12545)
                     /locus_tag="VN968_22915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HXL32119.1"
                     /translation="MPELAISAEKVVFLIEKTREFDVKESTSDPDSGSNSADDNMIDV
                     LQDNGEDPVVREITGFINAMTDDERIDLVALMRLGRGDGSIEEWDDLRREAADGDNRH
                     TASYLLGEPLVSDYLAEGLDAFGLDWSDELTTPV"
     gene            12680..13540
                     /locus_tag="VN968_22920"
     CDS             12680..13540
                     /locus_tag="VN968_22920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL32120.1"
                     /translation="MHWNVGKVKITKIVELETTGSTRFILPLATNEEIRKLPWLIPHF
                     ANEEGRLKMSIHSLVVETPSRRIVVDTGLGNDKEGRKVPTWNNRKDAFLETMTSAGFP
                     PDSIDTVLCTHLHVDHVGWNTKLAAGQWFPTFGNARYVFGKTEYEHWRDYRDESDKVA
                     VFNDSVKPIVDAGRAELVASDARLADEVTLIPTPGHSPGHMSIHIKSDGEEGLLTGDV
                     AHHPCQMAHLDWSSTADSDPVQSALTRRELFSRFADTPTLVIGGHFNAGHIRRDGDAF
                     KFMVWHMPNS"
     gene            13650..14492
                     /locus_tag="VN968_22925"
     CDS             13650..14492
                     /locus_tag="VN968_22925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXL32121.1"
                     /translation="MKLVRYGAIGQEKPGLIDKSGQLRDLSDHLKDLNGDAYAPASLA
                     KLAGLDTAKLPAVDGKPRLGAPVTGISKFVAIGLNYIDHAKETGNPIPPEPIFFLKAN
                     TALSGPNDPVEKPRGSTKLDWEVEIAAIIGTRAKYVSEADAINHVAGYCVCNDVSERN
                     FQIERQGQWTKGKSHDTFGPLGPWLVTKDEISDVQKLSMWLDVNGERRQRGSTSTMIF
                     TMAKCISYVSQFMTLLPGDIITTGTPPGVGTGMKPPQFLNAGDVVTLGIEGLGEQRQE
                     IIAA"
     gene            14675..15289
                     /locus_tag="VN968_22930"
     CDS             14675..15289
                     /locus_tag="VN968_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXL32122.1"
                     /translation="MKLTFSPASPFARKVRIAAIELGLIDNIEFVPTTVAPGQPNDAY
                     SQITPLKKLPALILDNGGVIVDSYVIVEYLDELAGGGKLVPASGPTRWKVKSDHSLLQ
                     GMLDAMLLCRYEKMVRPQGLQWQAWSDDHWNRAWSGMARFEKQADVLSGPFDIVQIGL
                     VCVLGYADFRFADCGWRKAYPKLDAFHEKMLARPSVKISVPPVA"
     gene            15306..16184
                     /locus_tag="VN968_22935"
     CDS             15306..16184
                     /locus_tag="VN968_22935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL32123.1"
                     /translation="MTLKLTVGDLTIHRLIEQETTFLPVLDMLPGLTPELLAENKPWM
                     QKAGAIDADDTLILCFQSYIVKTPHYTILVDSCIGNDKPRPLRPKWNMKTDDTYMHAL
                     AASGFSVVDIDFVMCTHLHVDHVGWNTRLENGRWVPTFPNARYVFGKSEFDYWTAQHA
                     NTPVPPFGDSVLPIVEVDKAEIVRDDYAIGDHVRILPTPGHTPGHVAFTFGRGKDDAV
                     FSGDLMHSPLQARYPELSAKFDVDQALAATTRRNFLERYCDTDTLCCTAHFPSPSTGK
                     IRRWGNGFSCEAVTAK"
     gene            complement(16355..16897)
                     /locus_tag="VN968_22940"
     CDS             complement(16355..16897)
                     /locus_tag="VN968_22940"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HXL32124.1"
                     /translation="MTDQHFALFDTAIGRCGIAWGARGINAVQLPMGNEEKTRARIRQ
                     RHGDIAEAPPPAEVQSAIDGIVELLAGKPNDLADIVLDLDGVPEFNRGVYDIARTIPP
                     GKTLTYGDIAKRLGGVELSRDVGQALGRNPCPIVVPCHRVLAAGGKPGGFSANGGVVT
                     KLKMLAIEGAVVNHTPSLFD"
     gene            complement(17087..17902)
                     /locus_tag="VN968_22945"
     CDS             complement(17087..17902)
                     /locus_tag="VN968_22945"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXL32125.1"
                     /translation="MSNDAVSFVGSIPQHYDQGMGPMIFVDYAADIAQRVAAGSPARV
                     LETAAGTGIVTRKLRDVLPAATRLTATDLNPPMLDIASAKFRAGEQVEFQPADAMALP
                     FADGSFDAVVCQFGVMFFPDKDKSYSEVLRVLAPGGRYVFSVWDSHRYNSFGRLAHQV
                     AGSFFPADPPPFYSVPFSCHQIDPIKESLIEVGFTGINAAVVSLNKEVPDTARFARAA
                     IYGNPLIDQIRTRGGVEPELIVGALAREYRREFGADPGRMPLQAIVFSAVKRS"
     gene            18054..18725
                     /locus_tag="VN968_22950"
     CDS             18054..18725
                     /locus_tag="VN968_22950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXL32126.1"
                     /translation="MIPLDPTPNLIDQVYARILEAIIDRTLPPGHRIRQNELAEKLGV
                     SRQPVSHALHLLHRQGLVAESGRKGFAVTQLDPMRIRQLYEVRGAIDALAARLAAARV
                     KTDAAGHMHLEAALRAGRATDKKTSLSQLITLDVDFHRAIYRLAGNPAIEEMIAPQWP
                     HMKRSMATVLAELDYRLSAWTEHEAITAHILSGNANAAEGAALAHAMSAGRMTEERLK
                     GLEAA"
     gene            18860..>19303
                     /locus_tag="VN968_22955"
     CDS             18860..>19303
                     /locus_tag="VN968_22955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXL32127.1"
                     /translation="MKLTQQQIDDFNRDGWLFLPELFSPEEVALLAHEAEGIYDTKRP
                     EVWREKSGAPRTAFAAHLYNEAFGLLGAHPRMIDPVEQIFGEKVYMHQYKINAKSAFT
                     GDVWQWHQDYGTWKRDDGMPEPRAMNIAIFLDEVMPINGPLMLVPK"
BASE COUNT         3825 a         5980 c         5838 g         3660 t
ORIGIN      
        1 ggacacgcgc ggatcgttct tgaaccagac cggatgcgca gtgccgcccc acggcacggc
       61 gatggcgccc tcatgctgac cgggaacgtt gacctcgaaa ttggcagtca catcccattc
      121 aacaaatttg ttctggacga ggtagtacca gttcgccttg aggatcgtga aaatggtctg
      181 cgtcgaggca taggtcggga accccttcca caacaccagg atgtcgagag tatcgaggcc
      241 gggcgtttcg aggcatatgt tggctgcgat ttcgccggtc gtgtacatct gcgcgatgtt
      301 gggcgcgagc agcaatcctt ttgccgcaaa tttttctccc cagcgttcct gcgccagcag
      361 cacccagtcc tgttcgccgg tcgtatcgga ataatgacag ccggcggcaa ggcaggcttc
      421 gacgacctcc gggccgtact tgatgaaagg gccgaccatg ttgctgacga cctttgcacc
      481 cctgaagagc ttggtcagcg cttcgacggt gtgctcaact ccgacgacct catagtcggc
      541 ggtttcaatg ccgggaatct tttcgacaac ggccttcact ttggcggcgt cgcggccggc
      601 ggcaataaag ggaatgttgt attcccgcag atattcacaa accagtcgcc cggtgtagcc
      661 agagacgcca tagacgatga cgggcttttt ctcgctcata ttcatatcct ctcgaatttc
      721 tattcgttgc gtttggcgat ctacatgccc atgccgccgt cgaccggcag gccggcgcca
      781 gtgatgaagc gcgcctcgtt ggagcaaagg aaaacagcgg cgtcggccat gtcgccgacc
      841 tcgcccaacc ggccaagcgg cgtctgctcg acgaccgagg cgacggcggc gccgacatcc
      901 ggtgcgagtc ccgcggcaac gatgtcgttg gccagttgca tgcccatatc ggtggggaca
      961 agcccgggat agagacagtt gaccctcacg ccgtaaccaa gcttgcccgc ttccatcgcg
     1021 gcgacccggg tcatgcggtc gacggccgat ttggtgccgg agtatcccgc aatggccggg
     1081 aatgcgatcg tggccgcgac cgatgcgata ttcacgatgg tcccgccggc acctgcgccc
     1141 ccgccaggtc gcatggcgcg aaacgcatgt ttcataccga gagcggtgcc gacgacgttg
     1201 acgtcgcaca tccggcgcag gtcctcagcc ctgagatcga cgacgagcga ggtaatctcg
     1261 atgcctgcat tgttgatcag gatgtcgtag ccaccgagcg tggcgatggt ggctgcgaca
     1321 gccttttccc attccgcgtc gtcggtgacg ttcagctcca cgaagccagt cttggcgcct
     1381 gtttttgcaa gacggcctgc agtttccttg ccgagatcgg cgaggatgtc gccgatcatc
     1441 acagaagcgc ccgccttcgt aagcgcctct gcgatcgcgg cgccgatgcc tcgcgcgccg
     1501 ccggtgacca tagccttgcg gcctgccaga cttttctcac tcatcgtatt ctcctcccgt
     1561 tgtgatttca ttgttgtgac gcgccgctgc ctgccgagcg ggggcggacc tgtcgcgcct
     1621 cgcccgctag ctgagaagaa aatctcggct gtctgtaacg aacggttgtg gtgtgcggtc
     1681 tgctctcctc cctgagcagc ttctaccggc tgcactgcct tgaaatttga cactcgtcca
     1741 agtttttctt gacgattgtc caaaaactat acacttgtca agcaaccctt gaccacgagg
     1801 gggaagaatg atttttcagg gcacctcagc gcattattcc cgccgaggtg acgcgatgat
     1861 tcggagttag cgtgcggaaa gaggctagag caacgcagcg gaaaattgaa acgacgagtc
     1921 ggccgagccg gccctcggac cgaatcgagc gtgcgcgtgt cgacaagttc agcgctcgac
     1981 gaatcgagct tgccgaggct gcgttggaaa ccttggccga gctcggctat gcgaggacca
     2041 gcctgcgcga gatcgcgcag aaatccgagt ttacccacgg cgtgctgcac tattacttca
     2101 ccgacaaggt cgacctgatc tgctgttgtg ttcgtcacta caaggcgaag tgcgtcacgc
     2161 ggtacgacca gatcacatcg gcggcgcaaa gccgcgacga actgatgaag ggttttctgg
     2221 aaaagctcgg ggagacggtt cgcgacgagg cccagatgca tcgcctctgg tacgaccttc
     2281 gctcgcagtc tctgtttgaa gaggcctttc gcgacgacgt cgccgagatc gacaagagcc
     2341 tggaagatat ggtgtggcgc atcgcgtccc gctttgcctc acttggcggc cgacagcctg
     2401 ccgtctcccg gggcgccctc tatgcgctgt ttgatggact cttccagcga tgtctgctgc
     2461 ggcatctctc gggcgatcgc aaggcgatcg cggacctgcg ggaagaagtg gcgcgcctgc
     2521 tcccgaccgt cgcatagcgg ctcgcggccg ttcgaattag cgcattgaaa ccgaagcggg
     2581 aacgttggca tacatttaat ggcgtcttct caaacggtga tcttctgcta gatatgcgac
     2641 ggcgctcgat cttgcgctac gcctcacgag cggcgtcttc ccgatttcgc agggtggtcc
     2701 ggtcacccgt cgatagccgt cttctcgatc gatctcaggg tcgacgagca gaccatccca
     2761 tagtcttgca atgtggcttg atgccggcgt cacggcagca gtttccaatt cgctcttctg
     2821 cggtctagcc tccgagatcg agcactcgct tccgaagcgc cggcgacaga tcaaggatcc
     2881 gaaacaggac ttttgtcgcg aaccggttcg ttagcgatcg tgtgtgaagc cgctccattt
     2941 gggctagcga cgcccgcaca gccgcgaacc catatttgat catttcttct tcataggcgg
     3001 aaagggcccc tagcaggtcc cggcgaccat tgagcacgtc acgcagcgcg tcccgtagca
     3061 cggcggcgtc acgcaacgcc gtattagcgc cgataccgcg gtagggcgtc atgttgtgga
     3121 gcgcgtcgcc cagcagagtg atccggctgg tcgtccaatg cggtatcggg actgacgatt
     3181 tcacaacaaa gctgctcagg gacgacgcat cggcatgcgc gaccaaatat cgtagatccg
     3241 cattccagtc cgccagctgg gcaagaactg cggctctcgc gtcttcgctc gctatcgcat
     3301 cggccgcgcc ttcgattcgc agggtgtcgc gatgtgctga gtaaccccat atgacgtatt
     3361 cgtcccggtc gaaaacggac aaatggtttg gtgaagtgca ctggcaccga aactcctgac
     3421 gccgaaccgc ggcgctatga tccggcaacc cgtcgtgtgc gacgtggacg atcgtcaaga
     3481 acggggcggg ctggcagccg catggaacag ctcgaacagt gtcaccggcc aggccggttg
     3541 caaggagttc caggcacgat gacaaccaat ggaacaaata tttgctgcga tgcatcgagc
     3601 ttcgatactg cgcgcccgtg aaggttcgga acgaaatccg ccgcttatcg ttcgcctatg
     3661 aggctgtaaa tttccgacag gaggtgccta tgaagcgatt tatcttggcg ctgttcgcgt
     3721 tggtgacctt gagcgcggca gcggtaacga cggcaaacgc cgtggagttc ggcgtcggtc
     3781 cgaatggtgt ttacgttggt ccggaccggg accgcgatta tcacgagcgg tactacaaca
     3841 actacggcag ctgccgggtt gtcattactc atcggatcaa tcgctttggc gaggacgtcg
     3901 aagtccgcaa acgcatttgc gattgatcgc gatgagataa cgggcgcgcc tcacttgttt
     3961 gggtgattgg cgcgcttttt tttggttcag gttcaccacc cccgggaagc ggagagctgc
     4021 gacgccccgg gatcttgcac catgcgtcgt gggcttcccg atatcgcagt ggtctgcacc
     4081 gcgagtgccg ccaccaggcg atggcggcac cgtgcggacg ctcatgctcc tgcgatctcg
     4141 ccgccgacgc gggcaaagaa cgggtggtcg cagaacacgt agttctcgca gacgtggccg
     4201 ctccgcgtgc gaacgcgatc gcagccgccg aattcggcct tgccgtccgg accggtcatt
     4261 gtcgcgaccc agcggatgaa ggtgaggtcg cccgacgaag catgctccga tcaaggaggg
     4321 gttagaattg ttgagacagt acacacaatg ggtgacagtg cacttgatag ccatcgcaat
     4381 tcacaggaat ggacagggcg gattgggatt gtgaccggat gggcaggatt tgggaatcgg
     4441 cggtattcgg cgccgatagc gtattccgaa gtggcgcgcc gcggccgacg aagatgggca
     4501 ctattgcttc gccgtggcgc tatgatgccg cagcccggca cgcgctccaa tcggagatac
     4561 tgcgacggcc tgcgatcttg cgctgcgact catgggctgg gcgccttgcc aatcttgcaa
     4621 tgtggtccgc ttacttcagt gatgaccttg ccccgcttca gcgtgcgctg tcagctgcgc
     4681 gagccgcgcc ttgccgagct tgctcagctc tggcttcaac acacgtcaga tgttccgctt
     4741 tgaccgcagg atcgattgcc cctgtttgag gcgctgcaag gcttcatcaa ccttgaggtc
     4801 atcttcgtcg tcgagaacag cgggatcaag gacacgtgcg ctcgttgtgc tgcgccgcgc
     4861 cctgatcgcc tgtttggcag cgttcttgta gccaatcggc aagcagtagc cattcgcgca
     4921 ttccatatcc gcaacaccgc cgacttcggc ccagatgtcc ctgccatgaa aaagtgtggt
     4981 tttcaaatct ccggtaacca ccaggcttga atcttcgtag gcattcaaga ccagctcact
     5041 cgcgttcaga ttgccgtcga tgaatgtgga tttgccgtag tggccggaaa atgcgcggca
     5101 agtcacgttg ccgatgacga tgaacaggcc gccttcgtcg aacccttccg gattgttcaa
     5161 gtcgatgagc ccgtcgactg tcagatcgcc gacaacgagc gtgcaatatc cgggagcctc
     5221 aaaatgtccg gaggtggtaa gagggccgcg atgaacgaag cagtgatcgt cgcccgaggg
     5281 cggaaacccc atgtatcgtc ttatgaacgc ctcggacgaa gaactgcccg cgatgacgtc
     5341 gtcgaattca tccccggtga gttgttcaaa atttgccatc gtcggatctc ctgcccctct
     5401 tcgctacgct acgtctcgcg gcgccaagcg ccagtcggaa ggtgacgaaa aagcccatca
     5461 ccaccgcacc ggctggtgcc aaaatgaatt caataccaac ggccttggca ccgttgccgt
     5521 cagcgacatg gaacggctgg gcatagacca tccagtcgaa atagaccgcc gcatagccga
     5581 tcacgagacc aagaccgaag acacaccaca agaacaccgc attcctacat cgcccgatcc
     5641 agtacgcccg aatctgaatg caggttggtc gcgcgagagc gacggacggt tcgatgcgaa
     5701 tggctggctc atgctatgat ttcagaaaac tgcggatgtc atgtttgtga cggcaattgc
     5761 gactccacga aatgcgcgct gattgcacgc aagaaggata cacctatgcc aatagccccc
     5821 cgagccgaat tccggcatcg ccgccgccaa gaacgtgtca tcgcattgcg gcggagtccc
     5881 agtcgggaca accgggaccc cgccaatcgt agtgccgatg gccgtatttg gcggcaatcg
     5941 cgaattccga agtggcgcgc ccgaagagat tcgaactcct gacccctaga ttcgtagtct
     6001 ggaagcagcc aaaatgcgcg gctcatggtg taagtggcca cgatcgcagt gggccactga
     6061 tcacaattga gtcgctgcgt cgcgcgcaac cccgccgatt ttacgctgca actttcctcc
     6121 tgccgtttcc acgaacgaaa tttcaggcct aaatccggat agcaaaaagc cctcaaaaac
     6181 gatgtgtttt caagggcttg atgtctggtt gcggggatag gatttgaacc tatgaccttc
     6241 aggttatgag cctgacgagc taccgggctg ctccaccccg cgataaacca ttgcgcgcct
     6301 ttgcaaaaaa ccgatgccga aacgaatttg gccaacgccc aaaggcgccg atcgatcccg
     6361 tccggaggct tcctgagaag gcaacccggg cgaacgccct tgggtgcgag cggtatgtat
     6421 caacgtgaac ccgctttgga aagggccatg acgcgacttt taatcatttt atgacggtca
     6481 agacgcgcat tcggcgccac cagaccggtc cgttaggcgc tgttgccaga aactccgcaa
     6541 aagcccagcc ttggaacaag agacttttgg gaaaggtcca tggaccagcc gctgaaaagc
     6601 ccagggcatc gggccttgga gcttgacgct gtcattccgg ggcgatgcca acgggtccgc
     6661 gcggcccgat gacaagctcc gcatcgaacc cggaatctcg agattccggg tctggtgctt
     6721 cggaccatcg cggaatgacg aaaaaataca aaactcaagg gatgcgcgtg acggatacat
     6781 tcgattttgt tgtggtgggc ggcggctccg ggggctgtgc ggtcgccggc cgtctctccg
     6841 aggatcccca gacctcggtg gcgttattgg aagccggcgg caggggcgac aattgggtgg
     6901 tcacgacgcc gggcgcgctg gcgctgatgg tcccgagcaa gctcaacaac tgggccttcg
     6961 atacggtgcc gcaagcgggc cttcaggggc ggatcggcta tcagccgcgc ggcaaggggc
     7021 tcggcggttc atccgcgatc aacgcgatgg tctatatccg cggccaccgc agcgactacg
     7081 accaatgggc gtcgctgggc aatgccggct ggtcctacgc tgacgtgttg ccctatttca
     7141 aacgttcgga agacaacgcc gatttcgatg gcgaatacca tggcaagggc ggccctttgc
     7201 ccgtcaacaa actgcgcacc gacaacccgg tgcagcaaac tttcctgcaa ggcgcgggcg
     7261 aggcgcagtt tcgcattcgc gaggatttca atgccgagga tcacgagggt ctcggcgtct
     7321 atcaggtgac gcagaggaac ggcgaacgct ggagcgccgc gcgggcctat gtccatcccc
     7381 atatggccac ccgcgccaat ttgcgcgtca agacccatgc gcagaccacc cgcatcctgt
     7441 tcgagggcaa gcgcgcggtc ggcgtcgaat acaggcaggg cgaagctaca aaacaaatgc
     7501 gcggccgccg cgaagtgatc ctctcctcgg gcgcgttcca gacgccgcaa ttgctcctgc
     7561 tgtccggggt cggcgacggc gccgcgcttg ccaggcacgg catcgcaacc acgcaccatc
     7621 tgcccggcgt cgggcaaaac ctgcaggatc atccggattt catcttcgcc tatctgtcgg
     7681 acagtcccta tctcaccggc atgtcgtttc gcggcatcgc gcatcagctt cgcgccatca
     7741 tgcagtaccg ccgcgaacgg cgcgggccga tgacttccaa ttttgcggaa tgcggcggat
     7801 ttctcaaaac cagcgccgat ctcgacatcc ccgacattca attgcatttc ggcatggcga
     7861 tcgtcgacga ccacggccgc aagcgccgct ggggccgcgg cttctcctgc catttctgcc
     7921 tgctgcggcc gaagagccgc ggcagcgtcg cgctctccag cgccgatcct cttgccgcgc
     7981 ccgcgatcga tccgaatttc ctcgggcatg ttgacgatct ggaatccatg gtcgcgggct
     8041 acaagaccac gcggcggctg atggagacgc cagcgctgcg ggcgttgcag aaaaaagaaa
     8101 tgttcacaac tggcgtcgag accgacgacg acatccgcgc gctcctgcgc gcgcgtgtcg
     8161 acaccgtgta tcacccggtc ggcaccgcca ggatgggcac cgatgcgctt gcggtggtcg
     8221 atccgacact gaaagttcac gggctggaag gcctgcgcat cgtcgatgcc tcagtgatgc
     8281 cgaccctgat cggcggcaac accaacgcgc cgaccatcat gatcggcgag aaagccgccg
     8341 atatgatcaa ggcggagctg tgggcaggtt gatgggcact atcgccgtca ttgcgaggag
     8401 cgtaagcgac gaagcaatcc agttctcagc ttgttgctgt ggattgcgga gcctgtcatc
     8461 ggacgcgcat tcgcgcgacc cgttggctcg caatgacgtg gagggagttc tgcggcatat
     8521 caattcagca aaaacccgcc atcgacggtg acgacactac cggtcatata gcgcgaggcg
     8581 ttcgaggcca gcagcatgat ggcgccgtcg agatcggctt cggcaccgac ccggcgctgt
     8641 ggaatccgtt ttgtcagttt ctcgcccgcg ggcgacgacc agaattcgtg gttcatctcg
     8701 gtatcgatat agcccggcgc caaggcattg acgcggatgc ggttgccggc cagttccagc
     8761 gccatcgcct tggtggcctg gatgatgccg gccttggaga tcgtataggg cgagagaaac
     8821 ttcatcacgc ccaggccgag cacggaagcg atattgacga tgttgccttc ctgcttgcgc
     8881 gcgatcattc gccgcgccat ttcggtggcc gcgaaatacg ctcccttgag gttggcgctg
     8941 atgacggcat cccaatcctg ctcggtctgg tctattgcga gtttttcaat cgcaatgccg
     9001 gcattgttca cgagcacgct gaccgggccg agtgttgctt ccgcgctgtc gatcgctttc
     9061 ccgatcgaag caatatccgt cacgtccatc tgtaccgcga cggcgcggcc gccctgcccc
     9121 aagatttctt cctccaggcc cttgagcttg gcggtctggc gcgccgccag cgcgaccgca
     9181 gcgccatgcg cggacaacac ccgcgcgaat tgccggccca atccctgcga ggcgccggtg
     9241 acaagaatga tttctttact gacgtcgaat agaccggaca tggctggctt ggtcccctca
     9301 agggtcgcac gttcattgat acagacgatt ttatggatcg caaaccggtt tggttgacca
     9361 acaggaaccg cgaaccttcg gcgcaggcgg agaagtgtcc atgaacagtt tcgatgccgt
     9421 cgtctatctc ggattggtca ttgcggtcgt gaccggtttc aatgccggat tgctgcgcag
     9481 tgcggtcacg atcctggcct atctgctcgc gatgccgatc gcggtctggg cgatgtcgct
     9541 gatcggctct cagatcgacg gcgcactcgg ctcgacatgg gcgcagaatt ggctgctgtt
     9601 cttcgggatc tttctgatcg ccggcatggt gctgggaaag ctgatgcgaa tggcgctcga
     9661 cgaaaccatc ggctcggaag ccggtatcgc agaccgcctc gccggtgcgc tgctcggcgc
     9721 ggtgcgcgtc ggcctggtcg cgacgacgct ggtgctgatc ttcgatctcg tgccgtcaga
     9781 tcgccagcct tcgttcctgg tcggctcgca gctgcggccg ctattgtcgg cggccgggca
     9841 gaagggcttc aagtcgctgc cgccggatgt cacggcgtac atcgatcgcc tcaagaaaga
     9901 ccagcggata taagacctcg aaagtcggcc ctgtccgggc gccgcggccc cagcctctga
     9961 tatgcatttc ggccctgccc ggtcccctta tcaaggccgc caaggttggc tacaaggggc
    10021 cggaccggac ttcaaaaatc atctgatatg atgggagtga gacagtggat cttgggatca
    10081 aaggccgtcg cgcactggta tgcgcatcca gcaagggatt gggacgcgcc tgcgcgatgg
    10141 cgctggccgc cgagggcgtg catgtcacgc tgaccgcgcg cggcgccgag gcgctggcga
    10201 aaaccgccgc cgagatccgc aaagccagcc ccggcgtcac ggtaacggaa gtcgccggcg
    10261 acatcacgac gcccgcgggc cgcgaggccg cgctaaaagc ctgtcccgat ccggatatcc
    10321 tggtcaacaa cgccggcggc ccgccgcccg gcgatttccg caactggacc cgcgacgact
    10381 ggatcaaggc gatcgatgcc aacatgctga cgccgatcga actgatcaag gcgacggtgg
    10441 acggcatgat ggcccgaaaa ttcggccgca ttgtcaacat cacctccgcc gcggtgaagg
    10501 cgccaatcga ggtactcgga ttatccaacg gcgcccgcgc cggcctcacc ggctttgtcg
    10561 ccggcatcgc ccgcaagacc gtgatcaaca acgtcaccat caacggcctg ttgcccggcc
    10621 ccttcaacac cgaccgcctg cgcggcccgg tcgcgaagat agacgcggac aagcgcggca
    10681 tcaccgtgga ccagttgctg gccgagcgcg ccaaactcaa tccagccggc cgcttcggcg
    10741 accccgagga gttcggccag gcctgcgcgt ttctctgcgg cgccaaggcg ggtttcatca
    10801 cgggccaaaa catcctgctc gatggcggcg cgtttccggg cacgctctga atgaaagtgc
    10861 tttgaatgaa ggccgtctgg tacgaacgaa ccggcgccgc gcctgatgtg ctgacctgtg
    10921 gcgatatgcc gacgccagag gccggtccgg gcgaagtccg ggtccggctg caggcctccg
    10981 gcgtcaatcc ggccgatgtc gggcgccgcg ccggcagcta tcgcgcgatg gagtttcccc
    11041 gcgtaattcc caacagcgac ggcgccggca ttgtcgacca gctcggtgcc ggcgttaccc
    11101 ggctcagcgt gggccagcgg gtctggctct ttaacggcca gcgcaacggc cgcgcatttg
    11161 gcacggcggc tgaatatgtc gcgctcgccg aacatctggt gacgccgctg gccgacaacg
    11221 tctcgttcgc ggccggtgcg acgctcggca ttcccggcat gaccgcgtgg tgcggcctgt
    11281 tcggcgatgg accgatcaca ggccagacgg ttctggtcac cggcggcgcg ggcgcggtcg
    11341 gccactacgc cgtgcagctg gcgaaatggg gcggcgcgcg cgtgatcgcc accgtcagct
    11401 catcgttcaa ggccgagcag gcgcggctcg ccggcgccga tctcgtcatc gactacaaga
    11461 ccgaggacgt catcgccaag gcgatggcgt tcaccgagca gcgcggcgtc aaccgggtcg
    11521 tcgatgtcga cttcggcggc aacgtcgcga caaccttgaa actgatggcg atgaattcca
    11581 ccatcgcggt ctacgccacc aacggcaatc gcagcccggt ggttccgatg cgcgagctga
    11641 tggagagatg cgttgcgctg cgcgcgctgg tgctgttcgc gctgccacag ccaatgcttg
    11701 cggcagcgca ggccgatatt tcaaaatggc ttgccgccgc cccgcgcatc cacaacatcg
    11761 ccgcgcagtt cgcgctgtcg gaaaccgcgc aggctcatct cgcggtggaa aaaggcgaca
    11821 agctcggcac cgtggtcgtc gattgtgcgc gttgaagtct gattcttgaa ttggatgcgt
    11881 cggagtctcg gcgtcacctc tccctagtgg gagaggtcgg atcgcgtcgg tgatgcgaag
    11941 catcaacggt gcgatccggg tgaggggtta cgatctatcg ataggccgta acccctcacc
    12001 ccaacccact cccgatggaa gtcggatatg tccgacttcg acaatttgag atgcccaact
    12061 cgggtaaacc cgagttgggt gggagaggga gcgctccgtg gtcgttgaga tatcacacac
    12121 agactaaact cttatcacac cggggtggtt agctcgtcgc tccagtccag gccgaaggca
    12181 tccagtcctt cggcgaggta gtcgctgacc aggggttcgc cgagcagata actcgcggtg
    12241 tgccggttat ctccgtcggc ggcctcgcgc cgcagatcgt cccactcttc gatgctgccg
    12301 tcgccgcgcc cgagccgcat cagcgccacc agatcgatcc gctcgtcgtc ggtcatggca
    12361 ttgatgaagc cggtgatctc gcgcacgacc ggatcctcgc cattatcctg cagcacatcg
    12421 atcatattgt cgtcggcgct gttcgacccg gaatccggat ccgacgtgct ctccttgacg
    12481 tcgaattcac gggttttctc gatcaaaaac acgacttttt cggccgaaat ggctagttct
    12541 ggcatcggtg cgctccttag cttcgcgtgc cccaccaacg ctcaggccag cgagatgatc
    12601 cattgcgcgg cgttacggaa accagcatct ttgcgccaag aaaccaaagg tgcaatggga
    12661 gaccaagggg aggcgccgga tgcactggaa tgtgggcaag gtcaaaatca cgaaaatcgt
    12721 cgaactggag acgaccggca gcacccgatt tatcctgccg ctggccacca acgaagaaat
    12781 ccggaaattg ccctggctga ttccccattt cgccaacgag gaaggtcgtc tcaaaatgtc
    12841 gatccattcg ctggtggtcg agacgccctc gcgccggatc gtggtcgaca ccgggcttgg
    12901 caatgacaag gagggccgaa aagtgccgac ctggaataac cggaaagatg ctttcctcga
    12961 aaccatgacg tcggcaggct tcccgcccga cagcatcgat accgtgcttt gcacccatct
    13021 gcatgtcgac catgtcggct ggaataccaa actcgccgcc ggccaatggt tcccgacctt
    13081 cggcaacgcg cgctatgtgt tcggaaaaac cgaatatgag cattggcgcg actaccgcga
    13141 cgaatccgat aaagtcgccg ttttcaacga ctccgtaaaa ccgatcgtcg atgccggcag
    13201 ggccgaactg gttgccagcg acgccaggct tgccgacgaa gtcaccctga tcccgacgcc
    13261 cggccacagc cccggccaca tgagcatcca catcaaatcg gacggcgaag aaggcctgct
    13321 taccggcgac gtcgcccatc atccctgcca gatggcacat ctcgactggt cgtcgaccgc
    13381 cgattccgat cccgttcagt cagccctgac gcgacgtgaa ttgttctcgc gttttgccga
    13441 cacgccgacg ctggtaatcg gcgggcattt caatgccggg cacatcaggc gcgacggcga
    13501 cgccttcaaa ttcatggttt ggcacatgcc taattcatga ccctgcaatg cggcgggcag
    13561 tcagcagttg aatttcaccg cgcactgttc catgaaacat ccaagtcaaa gagagccacc
    13621 caaggaaatc gtcaagggag tgtcgagaaa tgaagcttgt tcgttacggg gcgattggcc
    13681 aggaaaagcc cggcctgatc gataaatcgg gtcagctgcg cgatctgtcg gatcatttga
    13741 aggacctcaa tggcgatgcc tatgcgccgg cctcgctggc caagctggcc ggtctcgaca
    13801 ccgccaagct ccccgccgtc gatggtaaac cgcggctcgg cgcgccggtg accggcatct
    13861 cgaaattcgt cgcgatcggg ctgaattata tcgaccatgc caaggagacc ggaaatccca
    13921 taccaccgga gccgattttt ttcctgaagg ccaacaccgc cctctccggc cccaacgatc
    13981 ccgtggaaaa accgcgcggc tcgacaaaac tcgactggga agtcgaaatc gccgccatca
    14041 tcggcacccg cgccaaatac gtttccgagg ccgacgcgat caatcacgtc gccggctact
    14101 gcgtctgcaa cgacgtctcc gagcgcaatt tccagatcga acgtcagggc caatggacca
    14161 agggcaagtc gcacgacacc ttcggcccgc tcggcccctg gctcgtcacc aaggacgaga
    14221 tctcggacgt gcagaaatta tcgatgtggc tcgacgtcaa cggcgagcgc cgccagaggg
    14281 gatcgacctc gaccatgatc ttcaccatgg cgaagtgcat ttcctacgtc tcgcaattca
    14341 tgaccttgct gcccggcgac atcatcacca ccggcacccc gcccggcgtc ggcaccggca
    14401 tgaagccacc gcaattcctc aacgccggcg acgtggtcac gctcggcatc gaggggctcg
    14461 gcgagcagcg ccaggaaatc atcgcggcat gaggcgacgc gtgatgcgtc ccgcgaaagt
    14521 cgtcatgccc gggcttgtcc cgggcatcca cgtctagcaa cgtggcagct agaaaaacgt
    14581 ggatggccgg gcataggcga gcggaagcga cgccgtcctt cggacggcta tgtccggcca
    14641 tgacagagaa aaaagttatt taaggaatat cgaaatgaaa ctcaccttct cccccgcctc
    14701 gccattcgcc cgcaaggttc gcattgccgc gatcgaactc ggcctgatcg acaacatcga
    14761 gttcgtcccg accacggtcg cgccgggcca gcccaatgac gcgtactcgc aaatcacgcc
    14821 cttgaaaaaa ttgccggcgc tgatcctcga caatggcggt gtcatcgtcg attcctacgt
    14881 catcgtcgaa tatctcgatg aactcgctgg cggcggaaaa ctggtcccgg cgtccggccc
    14941 gacgcgctgg aaggtcaaga gcgatcattc gctgctgcag ggcatgctcg atgcgatgct
    15001 attgtgccga tacgaaaaaa tggtccggcc gcaaggcctg caatggcagg cgtggtccga
    15061 cgatcactgg aatcgggcct ggagcggcat ggcacgcttc gagaagcagg ccgacgtgct
    15121 gtcgggcccg ttcgacatcg tgcagatcgg ccttgtttgc gtgctcggct atgccgactt
    15181 ccgctttgcc gattgcggct ggcgcaaggc ctatcccaag ctcgatgcgt ttcatgaaaa
    15241 gatgctggcg cggccgtcgg tgaaaatctc ggtgccgccg gtggcctaaa aaacaggaag
    15301 tttccatgac tctcaaactc accgtcggcg atctcaccat tcatcgcctc atcgagcagg
    15361 aaaccacctt cctgccggta ctggacatgc tgccgggcct gacgccggag ttgctggccg
    15421 agaacaagcc atggatgcaa aaggccggcg cgatcgacgc cgacgatacc ctgatcctgt
    15481 gtttccagtc ctacatcgtc aagacgccgc attacaccat tctggtcgac agctgcatcg
    15541 gcaacgacaa gccgcgcccg ctgcgcccga aatggaacat gaagaccgac gatacctata
    15601 tgcacgcgct cgcggcctcg ggcttttccg tcgtcgacat tgatttcgtg atgtgtacgc
    15661 atttgcacgt cgatcatgtc ggctggaata cgcggctgga aaacggccgc tgggtgccga
    15721 cgttcccgaa cgcgcgctac gtgttcggca aaagcgagtt cgactactgg accgcacaac
    15781 acgccaacac gccggtgccg ccattcggcg acagcgtgct gccgatcgtc gaggtggata
    15841 aggccgagat cgtgcgcgac gattatgcga tcggcgacca tgtgcggatc ctgccgacgc
    15901 cggggcatac accgggacat gtcgcgttca ccttcggccg cggcaaggac gacgcggtct
    15961 tcagcggtga cctgatgcac tcgccgctgc aggcgcgcta tcccgagctt tcagcgaaat
    16021 tcgatgtcga tcaggcgctg gccgcaacga cgcggcgtaa tttcctggag cgctattgcg
    16081 acaccgacac gctgtgctgc accgcgcatt ttccctcgcc ctcgaccgga aaaatccggc
    16141 gctggggcaa tggattttcc tgcgaggcgg tgacggcgaa gtagattccg tttcgtgaac
    16201 tggaatcgcg ctgccacgta ctcggcgcca cctctcccct tgtgggagag cataggccgc
    16261 cttcggcggc cgttcttgaa aagaacgccg aagcgaagct tcggctacgt cgcatcgcca
    16321 gatgcgatcc gggtgagggg ctagccatgg cacctcagtc gaacagactc ggcgtgtgat
    16381 tgaccaccgc gccttcgatc gccagcatct tcagcttggt cacgacgccg ccattggcgg
    16441 aaaatccgcc gggcttgccg ccggcggcga gcacgcgatg gcagggcacc acgatcggac
    16501 agggattgcg cccgagcgcc tggccgacgt cgcgcgacag ttcgacgccg ccgagccgct
    16561 tggcgatatc gccataagtg agcgtcttgc ccggcgggat ggtgcgcgcg atgtcgtaga
    16621 cgccgcggtt gaactcgggc acgccgtcga gatcgagcac gatgtcggcg agatcattcg
    16681 gcttgcccgc gagcaattcc acgatgccgt cgatcgcgct ttgcacctcg gcaggcggcg
    16741 gcgcctccgc gatatcgcca tggcgctggc gaatgcgggc gcgggtcttc tcctcgttcc
    16801 ccatcggcaa ctgcaccgca ttgatgccgc gcgcgcccca cgcgatgccg cagcggccga
    16861 tcgcggtgtc gaatagtgca aaatgttggt cggtcatgac tggctccagg tcctgtggct
    16921 ccaaatcttg gcactcaatg gtttgagctt aacgcctggc tggaatatgg gcttggagat
    16981 tgttgcgatc cacccgaatt cccgggggaa cttggcagcg tcccatgaaa aacgacatag
    17041 cccaacctcc ttccccgcaa agcaggaagg agcaaaaccc tcgccgtcat gatcgcttga
    17101 cggcagaaaa cacgatcgcc tgcagcggca tgcgtccggg atcggcgccg aattcacgac
    17161 gatattcccg cgcgagcgcg ccgacgatca gttcaggctc gacgccaccc cgggtccgga
    17221 tctgatcgat cagcgggtta ccatagatcg ccgcgcgcgc gaacctcgct gtgtcgggaa
    17281 cctctttgtt caggctgacg acggcagcgt tgatgccggt gaagcccact tcgatcagtg
    17341 actccttgat cggatcgatc tgatggcagg agaacggcac gctgtagaac ggaggtggat
    17401 cggcagggaa aaaacttccc gcaacctgat gtgcgaggcg gccgaatgaa ttgtagcgat
    17461 gcgaatccca gacgctgaac acataacggc cgccgggagc tagcacgcgc aaaacctcgg
    17521 aataggattt gtccttgtcc ggaaagaaca tcacgccgaa ctggcacacg accgcatcga
    17581 agcttccatc cgcgaaggga agcgccatgg catcggcagg ctggaattcg acctgctcgc
    17641 cggctcgaaa tttcgcgctg gcgatatcga gcatcggcgg gttgagatcg gttgccgtca
    17701 ggcgtgtcgc agccggcaat acgtcgcgca gctttcgcgt gacgatgccg gtgccggccg
    17761 cggtctccag cacccgtgcg ggactgccgg cggcgacgcg ttgcgcgatg tcggcggcat
    17821 agtcgacaaa gatcatcggc cccatgccct gatcgtagtg ctgcggtatg ctgccgacga
    17881 aactcactgc gtcgttgctc atgatcgacc tccggcgcca cccgggcgag agcatagcgc
    17941 gacggcgctg gcccgctaaa gcgctcatgt ggtttgtggc ggcccttgac cccggcctcg
    18001 cctcccgcta gtttccacat tcggaattcc gtattccaat tggacacggt ggcatgatcc
    18061 cgctcgaccc gacccccaat ctgatcgacc aggtctatgc gcggattctg gaggccatta
    18121 tcgaccgcac gctgccgccg gggcatcgca tccggcagaa cgagcttgcg gaaaaactcg
    18181 gggtctcgcg ccagccggtt tcccacgcgc tgcatctgtt gcaccggcag ggccttgtcg
    18241 ccgaaagcgg ccgcaagggt tttgcggtca cgcagctcga ccccatgcgc atccgccagc
    18301 tctatgaagt gcgcggggcg atcgacgcgc tggcggccag gctcgcggcc gcgcgcgtca
    18361 agaccgatgc cgccgggcac atgcatctgg aggccgcctt gcgcgccggg cgcgcgaccg
    18421 acaagaagac ttcgctgtcg cagctgatca cgctcgatgt ggatttccac cgcgcgatct
    18481 atcggctcgc gggcaatccc gcgatcgagg aaatgatcgc gccgcagtgg ccgcatatga
    18541 agagatcgat ggcgaccgtg ctggccgaac tggattatcg cctgagcgca tggaccgaac
    18601 acgaggccat taccgcgcat attctgtccg gcaacgccaa cgccgcggaa ggcgcagcgc
    18661 tggcgcatgc gatgagcgcg ggacgaatga cggaagagag actgaaggga ttggaggcgg
    18721 cgtagtcaaa gcgccgtcat tccggggcgc gcgtgagcgc gaacccggaa tctcgatcaa
    18781 caacttctgg attccgggcc cacgcgaaga cgcgtgtccc ggaatgacga ggatcaataa
    18841 accaaaagga ggaaccacca tgaaactgac ccagcaacag atcgacgatt tcaaccgcga
    18901 cggctggctg tttttgccgg agttgttcag ccccgaagag gtggcgctgc tcgcccacga
    18961 ggcggagggg atctacgata ccaagcgccc ggaggtctgg cgcgaaaaaa gcggcgcgcc
    19021 gcgcacggcc tttgcagccc atctctacaa cgaggcgttc ggcctgctcg gcgcgcatcc
    19081 gcgcatgatc gatcccgtcg agcagatttt tggcgaaaag gtctacatgc accagtacaa
    19141 gatcaacgcc aaatcggcct tcaccggcga cgtctggcag tggcaccagg attacggcac
    19201 ctggaaacgc gacgacggca tgccggagcc gcgggcgatg aacatcgcga tctttctcga
    19261 cgaggtgatg ccgatcaatg gccccttgat gctggtgccg aaa
//
LOCUS       DATNOI010000569        12792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_423144,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000569 DATNOI010000000
VERSION     DATNOI010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12792
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423144"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VN968_22960"
     CDS             complement(<1..674)
                     /locus_tag="VN968_22960"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL32128.1"
                     /translation="MATVVVPSISAALAHATAEPSSGSGYAAGDGARLFFVRVGEGPL
                     MLFLHGHPDSALLYESQLREFGRDHLAVAPNLRGYAPSDAPEAVEAYAMPRLLGDVHG
                     LLDHFGMERCTLVGNDWGGYVAWVFASAFPTRVERLIILNAPHPAVHLRELRTNPAQI
                     KASQYEREFNTAAGPYPVWYNYYRADPIKVPASLEEGAANWAPDLAAHFFSGLGTPPA
                     TTSLQV"
     gene            complement(1042..1698)
                     /locus_tag="VN968_22965"
     CDS             complement(1042..1698)
                     /locus_tag="VN968_22965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL32129.1"
                     /translation="MTYLLPPLNALRAFEAAARHLSFKQAAHELHVTAGAVSQQVRLL
                     EERLGVQLFERRTRQVILTSAGETYLARVRQAFRCLAEATAELKPEGVTSLLHIGVHA
                     SFAVDGLRARLAQFRQAQPQVAIRISQPAGLHELLEGKVDVVIAERVQRCPGYRCEPL
                     ESGFLIGPLGTADCPEIETLRSCLLGHVELEPPTTARAPLVVDTASPAGRPSKPFFSV
                     "
     gene            1840..3075
                     /locus_tag="VN968_22970"
     CDS             1840..3075
                     /locus_tag="VN968_22970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL32130.1"
                     /translation="MPHEIPSIETSYSWIRLIASITLSTLGCVGMWSLVVALPAVQAD
                     FNVPRADATLPYTLTTIGFMIGGIIVGRLADRFGIFPPLAGGTILMSLGYVLTAFAPS
                     LPVFAVVSGVTIGLGAAATFAPLVADASLWFDRHRGLAISLATAGSSLAGVVWPTIIQ
                     HFIAMVGWRQTHVGIGIFCLATMLPLGLVLLRRPEFHKATLEAASTGNVNQAIGFGLA
                     PNATQGLLAFAGVCCCVAMSMPQVHLVAYCGDLGYGPARGAEMLAVMLGFGVASRLIF
                     GWVLNLIGGLPTLLLGSAMQAIALALYLPFNGLVSLYVVSAIFGLAQGGIVPSYAVIV
                     RELFPAQEAGLRVSLAISVTLAGMALGGWMAGAIYDWTGSYAAALVNGIAWNIVNMAV
                     AVWMLQRKHRRAVVACAAA"
     gene            complement(3339..3992)
                     /locus_tag="VN968_22975"
     CDS             complement(3339..3992)
                     /locus_tag="VN968_22975"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXL32131.1"
                     /translation="MDIEKVGLALRLRGARFLIDATHSAGVLTMDVRRLDPDFVMFPT
                     YKWLLGPYGRAFLYVARRHQGGIPLEQTAFGRRDVRAENAVYFKDVSYLSDARRFDMG
                     ERDHFISMEMASIGMEMMAEWGASAVVQRLLMLTERIADGLRGFGVSVPERRLRAPHI
                     LSLGFEDGMPAGLIERLATENVYVAPRLGRMRISPHVFNDEADADRFVAALAKRLRG"
     gene            complement(4008..4490)
                     /locus_tag="VN968_22980"
     CDS             complement(4008..4490)
                     /locus_tag="VN968_22980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXL32132.1"
                     /translation="MLASQRALFDVPRDICYLNSASYSPLPLRTQEAARAAVGRKATP
                     WTLEPSFANNQHERARAAAARLIQADPADMALIPSISYGVATAAKILTIERGARVIVL
                     ENDHSSPVLEWQTRADAQGFAVETVSRPEDGDWTSAVLAAIERSGAPPVCLASISSVH
                     "
     gene            complement(5452..6243)
                     /locus_tag="VN968_22985"
     CDS             complement(5452..6243)
                     /locus_tag="VN968_22985"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL32133.1"
                     /translation="METLDRIFDLTGRVAVITGGNGGIGLGIAQALAAAGCNVSIWGR
                     NTDKNKSAAATMAGCAGKVDTRVCDVSEPASVKAAMAATLQTFGRVDGCFANAGIGGG
                     GRHAFIDRTEEQWRKMFATNLDGVFHVFQAAARHMTERAEAGDRFGRLVATSSLASLF
                     GTARNEHYAATKAAINALVRALAVELARQGVTANAILPGWIKSDMTAGIMANEKFVGA
                     VMPRIPMRRFGEPGDFGGIAVYLMSKASSYHTADCFVIDGGYTAF"
     gene            6416..7207
                     /locus_tag="VN968_22990"
     CDS             6416..7207
                     /locus_tag="VN968_22990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase"
                     /protein_id="HXL32134.1"
                     /translation="MQFKHVTLDFDGPVAILKLDHQEVMNAVSIDMLSGLGEALDAID
                     DKRAEVRCLVITGAGRAFCTGANLQGRNNQKPGKSNAGAALETAFHPFLRRLRNLHCP
                     IVTSVNGPAAGAGMSFALMGDMILCARSSYFLQAFRRIGLVPDCGSTWLLPRLIGKAR
                     SVELSLMGERLPAEKALEWGLINRVYDDAALMEETMKLAHELANGPTIALSLIRKLYW
                     HSADNSFEDQLNLEFESQRIAGSAEDFKEGVTAFLEKRPAKFKGK"
     gene            7204..8190
                     /locus_tag="VN968_22995"
     CDS             7204..8190
                     /locus_tag="VN968_22995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HXL32135.1"
                     /translation="MIEQQLGRWVASWYPGAIGVANSLRLSGGASQETWSFDIMHPDG
                     LVGAILRRAPPGYGASPSRAAGLDAEATLMQLAHDAALPSPRVMHVLRPDEELGSGFI
                     MQRIEGETIARKILRDAQFAKARPVLARQLGKVAAGIHGLPALRLPKLRQMTATREIA
                     DLEREYRGFDWPRPVFELALRWLGDHDPGPPQEVTLVHGDFRHGNLIIGPEGLRAVLD
                     WELAHLGDPMEDLGWICVNSWRFGEIDRPVGGFGSREELFAGYEAAGRRVDPERVKFW
                     EVMGTLRWGVMCCGMMQRFRAGPDHSMERAMIGRRSSETEIDLLRLLAPRGK"
     gene            8192..8572
                     /locus_tag="VN968_23000"
     CDS             8192..8572
                     /locus_tag="VN968_23000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HXL32136.1"
                     /translation="MQDEPTPNELIKAVADFLRNEIAPVIKGHNAFKLRVGINALDLV
                     TRQLALAPDGDAAEAARLSQLLGLQGSLGELNRALADRIAKGEMDLQTPGLADHLWQT
                     TMDKLAVDQPNYASYKRELESKGG"
     gene            complement(8685..9464)
                     /locus_tag="VN968_23005"
     CDS             complement(8685..9464)
                     /locus_tag="VN968_23005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXL32137.1"
                     /translation="MDFKFSKVTRKGPITIVTLSRPEVYNALHIDAHFELNKIFDDFS
                     ADPEQWVAIVTGAGDKAFCAGNDLKWQAAGGKRGWDKGGFAGLTSRFDCDKPIIAAVN
                     GVAMGGGFEIALACDLIIASENATFALPEPRVGLAALAGGVHRLPRQIGLKRAMGMIL
                     TARHVSAKEGHELGFVNEVVPAGEALAAAERWAETICKNSPMSIRASKQAIQKGLSVS
                     LEQAIAEQREYPAVKAMAASQDYIEGPKAFSEKRPPKWMGK"
     gene            9682..10956
                     /locus_tag="VN968_23010"
     CDS             9682..10956
                     /locus_tag="VN968_23010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL32138.1"
                     /translation="MDFALPADLVAYLAELDQFIAREIKPIEAADDNIRFFDHRREWA
                     RTDFENGGLPRHEWEALLRRVKNLADDAGHLRFAIPKRYGGKDGSNLWMAVIREHFAA
                     KGLGLHNDLQNEHSIVGNLPLVTMLDRYGRDDQKAMIEGSITGKYRITFGLTEPEHGS
                     DATHMETRAVPATRDGVSGWVINGEKMWTTGMHVATHCALFARTSGNDGDARGITCFL
                     VPAKAKGVKIEEYMWTFNMPTDHPRVSFADVFVPDDALFGEIGRGLSLAQCFVHENRI
                     RQAASSLGAAVYCIEESVKYARQRKPFGRALAENQAIQWPLVELATQAEMLRLLIRKT
                     AWEMDKLTQAQVEHTLSDRVSMCNFWANRLCCEAADRAMQVHGGMGYSRHKPFEHIYR
                     HHRRYRITEGSEEIQKRKVAGFLFGYMGEGKH"
     gene            complement(11158..12030)
                     /locus_tag="VN968_23015"
     CDS             complement(11158..12030)
                     /locus_tag="VN968_23015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL32139.1"
                     /translation="MFNQQLLAGRRILVTGGGTGLGKSMAARFLELGAEVHICGRRKG
                     VCDETATELMDLYGGRVTSHGVDIRNPLAVDEMIGTIFTEGPLTDLINNAAGNFVSRT
                     QDLTPRGFDAIANIVMHGTFYVTHAVGRRWIAAKQPGNVVSITVTWVRNGSPYVVPSA
                     MSKSAIHAMTMSLAMEWGRYGIRLNTIAPGEIPTEGMSKRLNPGEQAGARTIARNPMG
                     RVGKMEELQNLAVFLISGGCDWINGETIAMDGGQALATGGNFYELRDWSDADWMRARD
                     QIKAQNEKDRAARG"
     gene            12140..>12792
                     /locus_tag="VN968_23020"
     CDS             12140..>12792
                     /locus_tag="VN968_23020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXL32140.1"
                     /translation="MDDRVKVTISEGVADVRLVRADKMNALDAAMFEALVATCDRLSR
                     EKGLRVVVLSGEGRAFCAGLDMGRFAAMNEKDGNGVAGGESRDLTARTHGLANVPQQA
                     VWGWRQLPVPVIAAIQGVAFGGGFQLALGADMRFLAPDARMSIMEIKWGLVPDMAGTP
                     ILANLVRDDILRELTYTGRIFSAQEALSYGLATRICDDPRAAAFDVAREIAGKSPEA"
BASE COUNT         2341 a         3940 c         4084 g         2427 t
ORIGIN      
        1 ttcacctgaa gcgaggtggt cgcgggtggc gtgccaagac cggagaagaa atgggcggcg
       61 agatctggtg cccagttggc ggcgccctcc tcgagcgagg ccggcacctt gatcggatcc
      121 gcccgatagt aattgtacca aacgggatat gggccggccg cagtgttgaa ctcccgttcg
      181 tactgagacg ccttgatctg ggccgggttg gtacgaagct cgcggaggtg aaccgccgga
      241 tgcggcgcgt tgagaataat caggcgttcg acccgggtcg gaaaagcgga ggcgaatacc
      301 caggcgacgt aaccgcccca atcattgccc acgagagtgc accgctccat gccgaaatgg
      361 tccaagagac cgtgcacatc accaagcaga cgcggcatgg cgtaagcctc gaccgcctcg
      421 ggtgcgtccg aaggggcgta gccgcgaagg ttgggagcta ccgcgaggtg atcgcggccg
      481 aattctctca gctgggattc gtagagcaga gcgctgtccg gatggccgtg aaggaacagc
      541 atgagtgggc cttcgccgac ccggacaaag aagagccgcg caccatcgcc ggcggcgtag
      601 ccgcttccag acgatggctc agctgtcgcg tgagccaaag ctgcgctaat cgacgggacg
      661 acgacggtcg ccatcccaag cctggcgcat gagacgagga ggcgccgacg ctggacctcg
      721 tgagttgctg tcacgcgacc tgccaactga gcccgcacga aattatcgac gcggcagcgg
      781 aaactataac aaccaagggc gaccattgaa agtaagttgt cgggagcgcc acggccgatg
      841 aagatgggca ctactgcttc gccgtagcac tatgatgctg gcgcctgccg cgcgatccaa
      901 tcggtagtgc tccgagcacc agcgattttg tactacgcct ttgcgcggtg ccgtcgtggg
      961 tgtcgaaaag cttcactagc tagactagcg gcttcctcaa tgaggccgaa atgcatgcaa
     1021 cgaggatgcg aagcaccgcg ttcagacgga aaagaaaggc ttagatggtc tgcctgctgg
     1081 gcttgccgtg tcgacgacca gtggcgccct ggctgtggta gggggctcaa gctcgacgtg
     1141 gccaagcagg caggaccgca gggtttcgat ctccggacaa tcggcggtgc cgagcgggcc
     1201 gatcaggaag ccactctcca gaggctcgca cctgtagcct ggacagcgct gcaccctttc
     1261 cgcgatcacg acatcgacct tgccttcgag caactcgtgc agccccgccg gttggctgat
     1321 gcgaatggcg acctgtggct gggcctggcg gaattgtgcc aggcgcgccc gcaagccatc
     1381 aacggcaaaa ctcgcgtgta ccccgatatg cagcagactt gtgacgccct caggcttgag
     1441 ttcggcagtg gcctcggcga ggcaacgaaa cgcctgacgt acccgcgcca gataggtttc
     1501 cccggcagaa gtcagaatga cctgccgtgt ccgtcgctcg aacagctgca cccctagccg
     1561 ctcctccagc aggcggactt gctggctgac ggcaccggca gtgacgtgca actcgtgggc
     1621 ggcctgcttg aagctgagat ggcgggcggc ggcctcaaag gcgcgcaggg cattgagtgg
     1681 cggcagcaga taggtcatgg ccggctccag gctatgcggt gggccgcaag gtgcatcagt
     1741 ttcgctcgtt ctacaaaaga gaaatcctgc tttgtcgctg acgaaggcgg gccgtaccta
     1801 tggccggctt acacgcaaag ctcaaggaaa cctaccacag tgccgcatga aattccgtcg
     1861 atcgagacgt cgtattcctg gattcgcctc atcgcctcca tcaccctcag cacactcggt
     1921 tgcgtcggca tgtggtctct ggtcgtggcg ctgccggcag tgcaagccga tttcaacgtg
     1981 cctcgcgccg acgcgacgtt gccctacaca ctgaccacca tcggcttcat gatcggcggc
     2041 atcattgtcg gccgattggc cgatcgattc ggcatcttcc caccgctcgc cggcggcacg
     2101 atcctgatga gcctcggcta cgtcctcacg gccttcgctc cgagcctgcc cgtcttcgcc
     2161 gttgtctccg gcgtcacgat cggcctcggc gccgcagcga ctttcgcgcc actggtggcc
     2221 gacgcctcgc tgtggttcga cagacaccgc ggcctcgcca tttccctggc gactgccggc
     2281 agctcccttg ccggcgtggt ctggccaacc atcatccaac atttcatcgc catggtcggt
     2341 tggcgccaga cccatgtcgg tatcggcatc ttctgcctgg caacgatgct gccgctcggc
     2401 ctggttctgc tgcggcggcc ggaatttcac aaggctacgc tcgaagcggc gagcaccggc
     2461 aatgtcaatc aagccatagg cttcggcctg gctccgaatg ccacccaggg tctgctcgcc
     2521 ttcgctggcg tgtgctgctg cgtcgccatg tcgatgccgc aggtccacct ggtcgcttac
     2581 tgcggcgatc tgggatacgg ccctgcgcgc ggcgccgaga tgctggcagt catgctcggc
     2641 tttggtgtgg cgagccgtct gatcttcggc tgggtgttga acctcatcgg tggcctgccg
     2701 accttgctgc tgggctcggc catgcaggcc attgcgctcg cgctttatct gccgttcaac
     2761 gggctggttt cgctctacgt cgtctccgcc attttcggcc tggcgcaggg tggcatcgtg
     2821 ccgagctatg cggtgatcgt gcgtgagctg tttccggcgc aggaggccgg tcttcgcgtc
     2881 agcctcgcca tctcggtcac tctcgccggc atggcgctag ggggctggat ggccggcgcg
     2941 atctacgact ggaccggttc ctatgcagcg gcgctggtca atggcatcgc ctggaacatc
     3001 gtgaacatgg ctgtcgcagt ctggatgcta cagcgcaagc atcggcgtgc tgtcgtcgcc
     3061 tgcgccgcgg cctagactag acttgcgacc tggcacgaat tacggtgaca gtgcacttaa
     3121 ctcgtcagcg cgatctattg agattgcgag ccacgcggcc ggcggatggc aacgcgacgg
     3181 cttgcatcgg aagaacggcg agtttcggcg tcaatagcgc atttccgaac tggcgcgccc
     3241 gaggagattc gaactcttct caccacagat tcgtcgtacg tccgttcgta gcgcagcact
     3301 cggctgccgc cacgggcgcc gggtttttgt aaggcaaact agccccgaag ccgctttgcc
     3361 aacgccgcaa cgaagcggtc ggcgtcggcc tcgtcgttga acacatgggg cgagatgcgc
     3421 atgcggccaa ggcgcggcgc gacgtagacg ttttcggtgg ccagtcgctc gatcaggccg
     3481 gccggcatcc catcctcaaa accgaggctc aggatatgcg gtgcccgcaa cctgcgctcg
     3541 ggcacgctga cgccgaaccc gcgcaagccg tccgcgatcc gttccgtcag catcagcagg
     3601 cgttgcacga cggccgatgc gccccattca gccatcattt ccatcccgat cgaggccatc
     3661 tccattgaaa tgaaatggtc gcgctcgccc atgtcaaacc gccgtgcatc ggaaaggtag
     3721 ctgacatcct tgaaatagac ggcattctcg gcgcgcacgt cacggcggcc gaacgcggtc
     3781 tgctcgagcg ggatgccgcc ctgatggcgc ctggcgacgt agagaaaggc ccggccgtag
     3841 gggccgagca gccatttgta ggtcggaaac atcacgaaat ccggatcgag acggcgcacg
     3901 tccatcgtca gcacaccggc gctgtgcgtc gcgtcgatca ggaagcgggc gccccgcagc
     3961 cgcagcgcca ggcctacttt ttcgatgtcg attagcccgc cgtccgacta gtgcaccgac
     4021 gagatcgagg caaggcagac cggaggagcg ccggatcgtt cgatcgccgc gagaaccgcc
     4081 gatgtccagt cgccgtcctc cggccggctc accgtctcga cggcaaaacc ctgcgcgtcg
     4141 gcgcgggttt gccattcgag caccggcgag gaatgatcgt tttccagcac gatgacgcgc
     4201 gcgccgcgct cgatggtcag aattttcgcg gccgtcgcga cgccataact gatcgagggg
     4261 atcagcgcca tgtcggcggg atcggcttgg atcaatcggg cagcggcagc ccgcgcgcgt
     4321 tcgtgctggt tgttggcgaa cgaaggctcg agcgtccagg gtgtggcctt gcgcccaacc
     4381 gcggcgcgcg cggcctcctg ggtccggagc ggcagcgggc tgtaggaggc cgaattcagg
     4441 tagcagatat cgcgcggcac gtcgaataaa gcgcgctgtg aggcaagcat gttgaaggtt
     4501 ctcttggttg ggacgttctg ttgcgacacc tcgactatgg cctatctcgg ccacgccggc
     4561 cacttggccg ctcgcgcccg ccgggcgatt tgttgtgatt tctcgatctg atttgcccgt
     4621 cgggcatttt tgtgtcgagt cctctttgca aaaatatttc gcttcacccg tcgggcaaat
     4681 catctctaca aattcgcgcc atcccacccc acaagagggg cgttggccat cgtcacgaac
     4741 gcggggaggg atgcggtgga cgcggcagcg ttttgcgtgc gacgggattg cagggcgggt
     4801 gatgagcctg tgagcgatcg acggcacgcg gacgagagac gattgcagcg tacggcgaag
     4861 tcgtgtggtc ctgacgcccc gacgctggcg tcaagttggc ggatgttatg tcggccctac
     4921 cgggcttggg acacaacgca agtccgctga cgacggtggc aaaagagccc ggtcaccggg
     4981 gagagcgcga aggaaaccgt taaaaccatt gcgtgcggga atgccgggcg attccggtgt
     5041 actcgtttta ctcgtgtgct ttttaccaat gccaagcgca cacgaggccg cgggtgcagc
     5101 gggcacccgg cattcccaac gccctcaaag ggggcggaag gttcatgcaa acctcgggcg
     5161 cttcgcgcgg cgaggtcgcg aacgtgtgtc agaaaacaat gcggctgttt gaaaatgaag
     5221 gcgggatctg ttcgaagcgc tccctacgct cgctcctcca aacccgctcg tcatgcccgg
     5281 acttgatccg ggcatccatc gatcttagta acaatttttt tcgaagaaga tggatcaccg
     5341 ggtcatcaag cgcgaagacg cgcggagcct gtcatcgggc cgcgctttgc gcggacccgt
     5401 tggcgctttc gcccggtgct gacaatctca attggtatga cggtcatcac ctcaaaacgc
     5461 cgtatacccg ccgtcgatca caaaacaatc cgcggtatga tacgacgacg ccttgctcat
     5521 cagatacacc gcgatgccgc cgaaatcgcc gggctcgccg aaccgccgca tcggaatccg
     5581 cggcatcaca gcgcctacga acttttcgtt ggccatgatg ccggcggtca tgtcgctctt
     5641 gatccagccc ggcaggatgg cattggcggt gacgccctgg cgcgccagtt cgaccgcgag
     5701 cgcgcggacc agcgcgttga tggcggcctt ggtcgcggcg tagtgttcgt ttcgcgcggt
     5761 gccgaacagc gacgccaggc tcgaggtcgc gaccagccgg ccaaagcgat ctcctgcttc
     5821 ggcgcgttcg gtcatgtggc gcgccgccgc ctgaaacacg tgaaagacgc cgtcgagatt
     5881 ggtggcaaac atcttgcgcc attgttcttc ggtgcggtcg atgaaggcgt gccgtccgcc
     5941 accgccgatg ccggcattgg caaaacagcc atcgacccgg ccgaatgtct gaagcgtggc
     6001 cgccatcgcg gctttcacgg aggcgggctc gctgacatcg caaacgcggg tgtcgacctt
     6061 gccggcgcag ccggccatgg tcgcggccgc actcttgttc ttgtccgtgt tgcggcccca
     6121 gatcgagacg ttgcagccgg cagcagccag cgcctgcgcg atgccaagac cgatgccgcc
     6181 attgccaccg gtaataacgg ccacgcggcc cgtgagatca aagatgcggt caagcgtttc
     6241 cattgggcgt ttccattttg gtttggcgcg cgttgggtcg tgtgtccgat ggcgggcagt
     6301 cgtcccgata ccaaccatgg acaagcgcgc ccagaaaatc aaatatggct ttcagatcga
     6361 aatcctgacg caccgcggca ctgacaacgc ggggtcacaa ctccagagga aacccatgca
     6421 gttcaaacac gtcaccctcg atttcgacgg tccggttgcc atcctgaagc tcgatcacca
     6481 ggaggtgatg aacgcggtat cgatcgacat gctcagcggt ctcggcgagg cgctcgatgc
     6541 catcgatgac aagcgagcgg aagtccgttg tctggtcatc accggcgcgg gacgcgcgtt
     6601 ctgcaccggc gccaatttgc agggccgcaa caaccagaag ccgggcaaga gcaatgccgg
     6661 tgccgcgctg gaaaccgcgt tccatccgtt cctgcgccgt ctgcgcaacc tgcattgccc
     6721 gatcgtcacc tccgtcaatg gtcccgccgc cggcgccggc atgagctttg cgctgatggg
     6781 cgacatgatc ctgtgcgcgc gctcatccta tttcctgcag gcattccgcc gcattggcct
     6841 cgtgcccgac tgcggatcga cctggctgct gccgcggctg atcggcaagg cgcgctcggt
     6901 cgaactgtcg ctgatgggcg aacggcttcc ggcggagaag gcgctggaat gggggctcat
     6961 caaccgggtc tatgacgacg cggccttgat ggaggagacc atgaagctcg cgcacgaact
     7021 ggcgaacggc ccgacgatcg cgctgtcgct gatccgcaag ctttattggc acagtgcaga
     7081 caattcattc gaggatcagc tcaacctcga attcgagtca cagcggatcg ccggctcggc
     7141 ggaagatttc aaggaaggcg tcaccgcgtt cctggaaaaa cgtccggcga agttcaaagg
     7201 caaatgatcg aacagcaact cgggcgttgg gtcgcctcct ggtacccggg cgcgatcggc
     7261 gtagccaact cactaaggct ctccggcggc gctagccaag aaacctggtc gttcgacatc
     7321 atgcatcccg atggtcttgt cggcgcaatc ctgcgtcgcg cgccgccggg ttatggcgcg
     7381 tcgccctcgc gggcggccgg gctcgatgcc gaggcgacgc tgatgcagct cgcccacgat
     7441 gccgccctgc cgtcaccacg cgtgatgcat gtgctgcggc cggacgagga gctcggcagc
     7501 ggcttcatca tgcagcggat cgagggcgag accatcgccc gcaagatcct gcgcgacgcg
     7561 cagttcgcca aggcgcgacc ggtcctggcg cggcagctcg gcaaggttgc ggccggtatc
     7621 catggcttgc ccgctttgag actgccaaaa cttcgccaga tgacggcaac cagggagatc
     7681 gccgatctgg agcgggaata ccgcggtttc gattggccgc ggccggtgtt cgaactggcg
     7741 ctgcgctggc ttggggatca cgaccccggt ccgccccagg aggtgacgct ggtgcacggc
     7801 gatttccgcc acggcaatct catcatcggc cccgagggat tgcgcgcggt gctggattgg
     7861 gagctcgcgc acctcggcga tcccatggaa gacctcggat ggatctgcgt caattcgtgg
     7921 cgttttggcg agatcgacag accggtcggc ggctttggtt cgcgcgagga attattcgcg
     7981 ggctacgagg cggccggtcg ccgcgtcgat cctgaacgtg tgaaattctg ggaagtgatg
     8041 gggacgcttc gctggggcgt gatgtgctgc ggcatgatgc agcgctttcg cgccggcccc
     8101 gaccattcga tggaacgcgc gatgatcgga cggcgttcgt cggagaccga gattgatttg
     8161 ttgcggctct tagctccgcg aggaaagtaa aatgcaggac gaacccacgc cgaatgagtt
     8221 gatcaaggcc gttgcggatt tcctgcgcaa cgagattgcg cccgtgatca agggacataa
     8281 cgccttcaag ctgcgggtag ggatcaatgc actcgatctg gtgacgcggc agttggcgct
     8341 ggcgcccgac ggcgatgcgg ccgaagccgc gcggctatcg caattgctgg gcctgcaagg
     8401 ctcgctcggc gaactcaatc gcgcgctcgc cgatcgcatc gcgaagggtg agatggattt
     8461 gcagacgccc gggcttgctg atcatctgtg gcagaccacg atggacaagc tcgcggtcga
     8521 tcagccgaat tatgcgtcgt ataagagaga gctggagagc aaaggtggct agcgctgtca
     8581 ttccgggatg gtccgaagga ccagacctca gatgtgcaat tgcacatcgg ggaatctcga
     8641 gattccgggt tcgatgcttc gcatcgcccc ggaatgacgt atcgctactt ccccatccat
     8701 ttcggcggac gcttttccga aaatgccttc ggcccttcga tgtaatcctg cgacgccgcc
     8761 atcgccttca ccgcgggata ctcgcgctgt tcggcgatcg cctgctccag cgacaccgag
     8821 aggcccttct gaatggcctg cttggaggcg cggatcgaca tcgggctgtt cttgcagatc
     8881 gtctccgccc agcgctcagc ggctgccagc gcttcgccgg ctggaaccac ctcattcaca
     8941 aaaccgagtt cgtggccttc ctttgcggag acatggcgcg cggtgaggat catgcccatc
     9001 gcgcgcttca gcccgatctg gcgcggtagc cggtgcacgc cgccggcgag cgcggcgagg
     9061 ccgacgcgcg gttcgggcag cgcgaaggtg gcattctccg aggcaatgat gaggtcgcag
     9121 gccagcgcaa tttcaaaacc gccgcccatc gcgacgccgt tgacggcggc gatgatcggt
     9181 ttgtcgcagt cgaagcgcga ggtgaggcct gcgaatccgc ccttgtccca accgcgtttt
     9241 ccaccggccg cctgccattt cagatcgttg ccggcgcaga acgccttgtc cccggcgccg
     9301 gtgacgatcg cgacccattg ctccggatcg gcggaaaaat cgtcgaatat cttgttcagc
     9361 tcgaaatgcg cgtcgatatg cagcgcgttg taaacttcgg gccgcgacag cgtcacgatc
     9421 gtgatcgggc ccttgcgcgt gacttttgag aatttgaaat ccatgtttgc tcccgtcgtt
     9481 ttttcgcagc gaggaattcg tggcgtcaat accagcttgg ccccgccatc cgataccatc
     9541 caattacgca ggtaacctcc gcgtcacaag cccaattccg tcgcttgact tggtctatcg
     9601 ttctcacctt aatcgaatgg cgcgggcctg tttcgtgtgg ccttgcgcta acaacaaaaa
     9661 aacacattcg ggagaactgc cttggatttc gcattgccgg ccgacctggt cgcgtatctc
     9721 gccgagctcg atcagttcat cgcgcgcgag atcaaaccga tcgaagcagc ggacgacaat
     9781 atccgtttct tcgatcatcg ccgcgaatgg gcacgcaccg atttcgagaa tggcgggctg
     9841 ccgcggcatg agtgggaagc gctgctgcga cgggtgaaaa atcttgccga cgatgccggg
     9901 cacctgcgct ttgcgatccc gaaacgctac ggcggcaagg atggctccaa tctctggatg
     9961 gccgtgatcc gcgagcattt tgccgccaag ggacttggcc tgcataacga tctgcagaac
    10021 gaacattcca tcgtcggcaa cctgccgctg gtcaccatgc tcgaccgcta cggccgcgac
    10081 gaccagaagg cgatgatcga aggttccatc accggaaaat accgcatcac ctttggcctc
    10141 accgagcccg aacatggttc ggacgcaacc cacatggaaa cccgcgcggt gccggcaacc
    10201 cgcgatggcg tctcgggctg ggtcatcaac ggcgaaaaga tgtggaccac aggcatgcat
    10261 gtcgcgaccc attgcgcgct gttcgcccgc acgtcaggca acgacggcga tgcgcgcggc
    10321 atcacctgct ttctggtgcc ggcgaaagcc aagggcgtga agatcgaaga atatatgtgg
    10381 acttttaaca tgccgaccga tcatccgcgc gtcagttttg ccgacgtgtt cgtacctgac
    10441 gatgcgctgt tcggcgaaat cggccgcgga ttgtcgctgg cgcaatgctt tgtgcatgaa
    10501 aaccgcatcc ggcaagcggc gagttcgctg ggtgctgccg tgtactgcat cgaggaaagt
    10561 gtgaaatacg cacgccagcg taaaccgttc ggtcgcgcgc ttgctgaaaa ccaggcgatc
    10621 cagtggccgc tggtcgagct ggcgacgcag gcggagatgc tgcggctctt gattcgcaag
    10681 accgcgtggg agatggacaa gctcacgcag gcgcaggtcg agcacacgct gtcagaccgg
    10741 gtctcgatgt gtaatttctg ggccaaccgg ctgtgctgcg aggccgccga ccgcgccatg
    10801 caggtgcatg gcggcatggg ctattcacgc cacaagccgt tcgaacacat ctaccgccac
    10861 caccgccgct accgcatcac cgaaggcagc gaggaaatcc agaagcgcaa agtggcagga
    10921 tttttgttcg gctatatggg ggaagggaag cattgaggcg gtgttggtgc ggatgcgccg
    10981 cgttgttcca ttggatgcac gacgtagctc caatgaatac gtcgtcgccg cgaaggcgga
    11041 gacccataac ccctggcgct cgttgtggcg gcacaacggt tcaacaacga tgcttacgct
    11101 tcccgtgaca ttggtgacac ggcgtatggg tcccgcgctc gccgggacga cgtgaagtca
    11161 cccccgcgca gcgcggtcct tctcgttctg cgccttgatc tggtcgcgcg cccgcatcca
    11221 gtcggcgtcg gaccagtcgc gcaattcata gaagtttccg cccgttgcca gcgcctggcc
    11281 gccgtccatg gcgatggtct cgccgttgat ccagtcgcag ccgccggaaa tcaggaacac
    11341 cgccaaattc tgcagttctt ccatcttgcc gacgcggccc atcggattcc tggcgatggt
    11401 gcgcgcgccg gcttgttcgc cggggttgag gcgcttgctc atgccctcgg tcggaatttc
    11461 gccgggcgcg atggtgttga gccggatgcc gtagcgtccc cattccattg ccagcgacat
    11521 ggtcatggca tggatcgccg acttgctcat cgccgacggc accacatagg ggctgccgtt
    11581 gcggacccat gtcacggtga tcgacaccac attgccgggt tgcttggcgg cgatccagcg
    11641 ccggcctacc gcgtgcgtca catagaacgt gccgtgcatg acgatgttgg cgatcgcatc
    11701 gaagccgcgc ggcgtcaggt cctgggttcg cgagacgaaa ttgccggcgg cgttgttgat
    11761 gaggtcggtg aggggacctt cggtgaagat ggtgccgatc atctcatcga ccgcgagcgg
    11821 gtttctgatg tcgacgccgt ggctcgtcac cctgccgcca tacagatcca tcagctcggt
    11881 cgcggtttcg tcgcagacgc ccttgcggcg gccgcaaata tgaacctcgg cgccgagttc
    11941 aaggaagcgc gcggccatcg acttgccgag cccggttccg ccgccggtga ccagaatgcg
    12001 ccgcccggca agaagctgtt ggttgaacat ctggtgtttc cgccctgaag atttgttggt
    12061 taattggttg attgactaaa tccggacaac gcctttctgt aaagtggcaa ccacaaagaa
    12121 gagcaggaga gaaactctca tggatgatcg cgtcaaggtt acgatctcgg agggcgtcgc
    12181 cgacgtccgt ctggtgcggg cggacaagat gaatgcgctc gatgccgcga tgttcgaagc
    12241 ccttgttgca acctgcgacc ggctttcccg cgaaaaaggg ctgcgggtgg tggtgttatc
    12301 cggcgagggg cgggcttttt gcgccggcct cgacatgggc cgctttgcgg cgatgaacga
    12361 gaaggacggt aacggggtag cgggcggcga aagccgcgat ctgaccgcgc gtacccatgg
    12421 actggccaat gttccgcagc aggcggtatg gggatggcgg cagctgccgg tgccggtgat
    12481 tgccgcgatc cagggcgtgg cgttcggcgg cggctttcag ctcgcgctcg gcgccgacat
    12541 gcggtttctc gcgcccgacg cgcggatgtc gatcatggag atcaaatggg ggctggtgcc
    12601 ggacatggcg gggacgccga tcctggccaa tctcgtccgt gacgatatcc tgcgcgagct
    12661 gacctatacc gggcggatct tttccgcgca ggaagccctg agctacggcc tcgcgacgcg
    12721 catctgcgat gacccgcgtg ccgctgcgtt cgacgtcgcg cgcgaaatcg ccggcaagag
    12781 tcctgaggcg at
//
LOCUS       DATNOI010000570         4731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2578612,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000570 DATNOI010000000
VERSION     DATNOI010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2578612"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VN968_23025"
     CDS             <1..186
                     /locus_tag="VN968_23025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HXL32141.1"
                     /translation="RFHLEDIVLKEAWTGPAALGLFPHALADVARLPVREVVSALHFK
                     ADLTLGLGTVAFDYMKR"
     gene            complement(355..1788)
                     /locus_tag="VN968_23030"
     CDS             complement(355..1788)
                     /locus_tag="VN968_23030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homospermidine synthase"
                     /protein_id="HXL32142.1"
                     /translation="MSPSDQQIHARISGPIVMIGFGSIGKGTLPLIERHFEYDKSRFV
                     IIDPHEDGEIARQHGVRFIKQAITRDNYRELLVPLLTAGGGQGFCVNLSVDTSSVDIM
                     TLCRELGALYIDTVVEPWLGFYFDRNIGTEKRSNYALRETLLAAKRKSPGGPTAVSTC
                     GANPGMVSWFVKQALLDIARDTNTPFEEPKSREGWGRLAHKLGVKGIHIAERDTQRSK
                     RPRPMNIFVNTWSVEGFVAEGMQPAELGWGTHEKWMPDNGRAHDYGCGAAIYLLQPGA
                     NTRVRSWCPTPGAQYGFLVTHNESISIADYFTVRDGERVVYRPTCHYAYHPANDAVLS
                     LHEMFGAAGKMQPKWHILDENEIEDGIDELGVLLYGHGKNAYWYGSQLSIEETRDIAP
                     YQNATGMQVSSAVLAGMVWALENPTAGIVEADELDFHRCLQIQRPYLGPVKGIYTDWT
                     PLTDRPGLFPEDIDESDPWQFRNVLVR"
     gene            complement(1831..2220)
                     /locus_tag="VN968_23035"
     CDS             complement(1831..2220)
                     /locus_tag="VN968_23035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXL32143.1"
                     /translation="MSRRLISTGSPFEKTAGFSRAVIDGDFAFVAGTTGYDYATMTMP
                     ADITSQARNCFKTIEAALMEGGFAMTDIVRATYYVTDASNADAVLAVCGEVLGDIRPA
                     ATLLVVAGLLKPEMKVEIEVTAKRRTA"
     gene            complement(2465..3184)
                     /locus_tag="VN968_23040"
     CDS             complement(2465..3184)
                     /locus_tag="VN968_23040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HXL32144.1"
                     /translation="MFAIRAERASDVVAREALLDACFGANRHQRTCQRLRDGRAPAEG
                     LALSAVRQGLLSRIRSSHCLPQDRERTSNSIGTAEASKCACGGCFGNSPEETCREGVG
                     NFRNATRLVGTVRLWHVSAGGVPALVLGPLAVDPSCRKLGIGRALVGQALAAAKARGH
                     GAVLLLGDASYYARFGFSAVKTAELRLPGAFERDRLLGLELRDGALDGAWGMIVATGA
                     SGLKIGAARAKKALRPAPHAA"
     gene            complement(3352..4494)
                     /locus_tag="VN968_23045"
     CDS             complement(3352..4494)
                     /locus_tag="VN968_23045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HXL32145.1"
                     /translation="MTERIQEFLRNRRNEGQDVEPCLVVDLEVVRDNYQTFSKALPDS
                     RVFYAVKANPAPEVLSLLASMGSCFDCASVAEIQMALAAGAPADRISFGNTIKKERDI
                     ARAFALGIRLFAVDCTAEVEKTARAAPGAKVFCRILYDCAGAEWPLSRKFGCDPEMAV
                     EVLDLAKRLGLEPYGISFHVGSQQRKVKAWDRALAMASTVFRDCAERGINLSMVNMGG
                     GFPTKYLKDVPPVVQYGRSIFRALRKHFGNQIPETIIEPGRGMVGNAGVIETEVVLIS
                     RKSDEDEVRWVYLDIGKFGGLAETMDESIRYAIRTPHDGAEMTPCVLAGPTCDSADVL
                     YEKIPYPLPVTLEIGDKVLIEGTGAYTSTYSSVAFNGFPPLRTYHI"
BASE COUNT          858 a         1496 c         1545 g          832 t
ORIGIN      
        1 cgcttccatc tcgaagacat cgtcttgaag gaggcatgga ccggccccgc ggcgctcggc
       61 ctgttcccgc acgcgctggc cgacgtggcg cggctgccgg tgcgcgaagt ggtctccgcg
      121 ctgcatttca aggccgatct gacgctcggg ctgggcaccg tcgcgttcga ttatatgaag
      181 aggtgatgag cgcgatcgcg tcatcctcag tcgtcatgcc ccgcgaaagc ggggcatcca
      241 gtacgccgcg gcggttatga gaatcgagac gccggcgtct actggatcac ccgctttcgc
      301 gggtgatgac ggcgaatttt ttagaacacg cgacggtgac gttggtcgcc cgcctcaccg
      361 caccagcacg ttccgaaact gccagggatc gctttcatcg atatcttcgg ggaacagtcc
      421 gggccggtcg gtcagcggtg tccagtcggt ataaatgcct ttcaccgggc cgagatacgg
      481 ccgctggatt tgcaggcagc gatggaagtc cagctcgtcg gcctcgacga tgccggcggt
      541 aggattttcc agcgcccaca ccatgccggc gagcaccgcc gatgacacct gcatgccggt
      601 ggcgttctgg tagggcgcga tgtcgcgggt ttcctcgatc gaaagctgcg atccgtacca
      661 ataggcgttc ttgccatggc cgtagagcag cacgccaagc tcgtcgatcc cgtcctcgat
      721 ttcgttctcg tccagaatgt gccacttcgg ctgcatcttg ccggccgcgc cgaacatctc
      781 gtgcagcgac agcaccgcgt cgttggcggg gtgataggcg taatggcagg tcgggcggta
      841 caccaccctc tcgccgtcgc gcacggtgaa atagtcggcg atcgaaatcg attcgttgtg
      901 ggtgaccaga aagccgtatt gcgcgccggg cgtcgggcac catgagcgca cgcgggtgtt
      961 ggcgccgggc tgcaagagat agatcgcggc gccgcagccg tagtcgtggg cgcggccatt
     1021 gtccggcatc cacttttcgt gggtgcccca gccgagctcg gccggctgca tgccttcggc
     1081 aacaaaaccc tccaccgacc aggtgttgac gaagatgttc atcggcctcg gccgcttcga
     1141 acgctgggtg tcgcgctcgg cgatatggat gcccttgacg ccgagcttgt gggcgagacg
     1201 gccccagcct tcgcggcttt tcggttcctc gaacggcgtg ttggtatcgc gggcgatgtc
     1261 gagcagcgcc tgcttgacga accaggacac catgccggga ttggcgccgc aggtggagac
     1321 cgcggtcggt ccgcccgggc tcttgcgctt tgccgccagt agcgtctcac gcaacgcgta
     1381 gttcgagcgc ttttccgtgc cgatattgcg gtcgaaatag aagccgagcc acggctccac
     1441 gaccgtgtcg atgtaaagcg cgccgagctc gcggcacagc gtcatgatat cgaccgagga
     1501 agtgtccacc gacaggttga cgcaaaagcc ctgaccgccg ccggcggtca ggagcggcac
     1561 cagcagctcg cgataattgt cccgggtgat cgcctgtttg atgaaacgca cgccatgctg
     1621 ccgggcgatc tcgccgtcct catgcggatc gatgatgacg aagcgcgatt tgtcatattc
     1681 gaaatgccgt tcgatcagcg gcagggtgcc tttgccaatc gagccgaagc caatcatgac
     1741 aatcgggccg gaaattctcg cgtggatctg ctgatcggac gggctcatgg gtctgtctcc
     1801 ggaaaatagg gatgcgctga cgaagcggga tcaggcggtg cgccgcttgg cggtgacctc
     1861 gatctcgacc ttcatctcgg gcttcaggag cccggcgacg acgaggaggg ttgcggccgg
     1921 cctgatatcg ccaagcacct cgccgcagac cgcgagcacg gcgtccgcgt tgctcgcatc
     1981 agtgacgtaa taggtcgcgc ggacaatgtc ggtcatggcg aaaccaccct ccatcagcgc
     2041 ggcctcgatg gtcttgaagc agtttcgtgc ctggctcgtg atatctgccg gcatggtcat
     2101 ggtggcgtaa tcgtagccgg tagtgccggc gacgaaggcg aaatcgccgt cgatgacggc
     2161 gcggctaaag ccggccgtct tttcgaacgg cgaaccggtg gaaatcaggc ggcgagacat
     2221 cgggacctcg atttgggctt tgcggagcgg cgtttccaat tgcgggctgc gcagctttcg
     2281 ctatcgcaca ccgaacgata tgtcacgccg ggaagtggcg cgcagcccgg cttcgtcggc
     2341 gcgcaaatat gtcccggacg atcatcacgg ccagcaagat caaaaggatg ctcgcaaggg
     2401 ctctgccacg tgaattcggg gcgtcgcgcg ccatgggttg ttttgcggac atggtgccag
     2461 gcgatcaggc cgcgtgcggc gccggtcgca gcgccttctt cgcgcgcgca gccccaattt
     2521 tcagcccaga agcgccggtc gccacgatca tgccccaggc gccgtcgagc gcgccatcgc
     2581 gcagttcgag accgagcagg cggtcgcgtt cgaacgcacc agggagccgc agttcagcgg
     2641 tcttgaccgc cgagaagccg aagcgggcat aataggacgc gtcgccgagc aagagcacgg
     2701 cgccatgtcc gcgcgccttc gcggccgcca gcgcctgccc gaccagcgcc cttccgatgc
     2761 cgagcttgcg gcacgacgga tcgaccgcca gtgggccaag caccagcgcc ggcacgccgc
     2821 cagcgctgac gtgccacaac cggacagtgc cgaccaggcg tgtggcgttt cggaaattcc
     2881 ccacaccctc gcggcaagtc tcttctgggg aatttccgaa acatccgcca cacgcacact
     2941 ttgaagcttc tgctgtcccg atcgaattcg aagttcgctc ccggtcttgc ggcaagcaat
     3001 gcgaacttcg aattcgggac agcagaccct ggcgcacggc cgaaagcgcg aggccttcgg
     3061 caggcgcgcg tccgtcgcgc aggcgctggc aggtgcgctg gtggcgattt gcgccaaagc
     3121 aggcatccag gagcgcctcg cgcgccacga cgtccgaggc acgttccgca cggatcgcga
     3181 acagattagc gccggaggtg agggcaacgg gggtcttccg caaagcagtc atggcacgtc
     3241 agtcctcgct gctaccggca cgggccggcg cagcgttgtc agcaaattcg aaggtgaggg
     3301 aagggagccg gcgcaccggc tcccgattat cctggcctca gggaggcggc ctcagatatg
     3361 gtaggtccgc agcggcggga agccgttgaa cgccaccgac gagtaggtcg acgtataggc
     3421 cccggtgccc tcgatcagca ccttgtcgcc gatctccagc gtcaccggca gcgggtacgg
     3481 gatcttctcg tacagcacgt cggcggaatc gcaggtcggg cctgcgagca cgcacggcgt
     3541 catttcggcg ccgtcatgcg gggtgcggat ggcgtagcgg atcgactcgt ccatggtctc
     3601 ggcgaggccg ccgaacttgc cgatgtcgag atagacccag cgcacctcgt cctcgtcgct
     3661 cttccttgag atcagcacga cttcggtctc gatgacgccg gcattgccga ccatgccgcg
     3721 gcccggctcg atgatggtct ccgggatctg gttgccgaaa tgcttgcgca gcgcacggaa
     3781 gatcgaccgg ccgtactgca ccaccggagg gacatccttg aggtacttgg tcggaaatcc
     3841 gccgcccatg ttgaccatcg agaggttgat gccgcgctcg gcgcagtcgc ggaacacggt
     3901 cgaggccatc gccagcgcgc ggtcccacgc cttcaccttg cgctgctgcg agccgacatg
     3961 gaacgagatg ccatagggct ccaggccgag gcgcttggcg aggtcgagca cctccaccgc
     4021 catctccggg tcgcaaccga acttgcgcga cagcggccat tcggcgcccg cgcaatcgta
     4081 gaggatgcgg caaaacacct tggcgccggg ggcggcacgg gcggtctttt cgacttccgc
     4141 ggtgcaatcg acggcaaaca ggcgaatgcc gagcgcgaag gcgcgcgcga tgtcacgctc
     4201 cttcttgatc gtattgccaa aggaaatgcg atcggcgggc gcgccggcgg caagcgccat
     4261 ctggatttca gccaccgagg cgcaatcgaa gcacgagccc atcgaggcca gcagcgacag
     4321 cacttccggc gccgggttgg ccttcaccgc gtagaacacg cggctgtccg gcagcgcctt
     4381 cgagaatgtc tgatagttgt cgcgcaccac ttcgaggtcg acgacgagac acggctcgac
     4441 gtcctggccc tcgttgcggc ggttgcgcag gaattcctga atacgttcgg tcatagcacc
     4501 ctccaaacgg cccagcgacg ggacgcgttc aaatgtgatc tcggataaga gcgcttcggc
     4561 cacatcgcgc gatggaggcg caacgaggcc aaaaaactcg aaccgaattg tgctgccgtg
     4621 gattggttgg gaatgatccc gcccgcacac ctggcaatga aggacaagcc tcttcggtat
     4681 tcgcgccggc tggtggaaag ccggcagaga ccaaaaaagc ccgatccgtc g
//
LOCUS       DATNOI010000571        12734 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1305082,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000571 DATNOI010000000
VERSION     DATNOI010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305082"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VN968_23050"
     CDS             complement(<1..639)
                     /locus_tag="VN968_23050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HXL32146.1"
                     /translation="MHERERWQVIRAMLKQQLLVRIADVCRATGASEASVRRDFARLA
                     EMGSATRVRGGLEAMPGSQMASSDAPALATRSFEISRTLNVAAKRAIARAAVALCSDG
                     DAIIINGGTTTFEMGEFLRERRLKVLTNSYPLAETLIHHSNCRVALPGGEVYREQKLI
                     VSPFEEDAIQHYSASRMFMSAISIGPLGVIEGDPLIARAETKLLKRAEKLVVL"
     gene            829..2867
                     /locus_tag="VN968_23055"
                     /pseudo
     CDS             829..2867
                     /locus_tag="VN968_23055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
     gene            2892..4187
                     /gene="rhaI"
                     /locus_tag="VN968_23060"
     CDS             2892..4187
                     /gene="rhaI"
                     /locus_tag="VN968_23060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose catabolism isomerase"
                     /protein_id="HXL32147.1"
                     /translation="MTDFSISDDLLQAENAARKPALDEDYAALLRALGRRGIDGDALT
                     RRVMAFGVAIPTWGVGTGGTRFARFPGPGEPRHLFDKLEDCATIHHLVRATPTVSPHF
                     PWDATSDYAALREVAAKHGLAFDAVNSNTFQDQPNQEHSYKFGSLSHVKPAVRAQAIA
                     HNIDCIAIGRQLGSEALTVWIGDGSNFAGQSNMTLALDRYLDSMREIYAALPADWRVF
                     LEHKLYEPAFYSSVISDWGTSFAAAQELGPKAQCLVDLGHHAPNVNIEQIVARLVRFG
                     KLGGFHFNDSKYGDDDLDTGSVEPFRLFLVFNELIEARMRGAEGFAPAYMLDQSHNVT
                     DPIESLMQSAIELQRAHAQALIVDRSALAAAQAANDALGAHLELKRAYTADVSPILAM
                     ARLRAGGAIAPIETYRASGYRRHKAKERPPVARTSAGIV"
     gene            4524..4613
                     /locus_tag="VN968_23065"
     CDS             4524..4613
                     /locus_tag="VN968_23065"
                     /inference="COORDINATES: protein motif:HMM:NF012280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor
                     domain-containing protein"
                     /protein_id="HXL32148.1"
                     /translation="MLPVCGCNGKTYNNDCERQKQAGVGRILH"
     gene            complement(4739..5932)
                     /locus_tag="VN968_23070"
     CDS             complement(4739..5932)
                     /locus_tag="VN968_23070"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXL32149.1"
                     /translation="MSTNFSWAADAVLDGVVTSTPRVPGVVAMVTDRHRNIYEGAAGK
                     RRLDQPAGMTTDSIFAIFSTTKAITGTAVLQLVEEGKLDLDAPARRYAPEIGKLQVID
                     GFDAKGEPILRAPKRDVTTRMLMVHTSGLSYDFINHTYNRLAQEKGQPSVITASKACL
                     MTPLLFDPGERWEYGTNLDWCGQIVEAITDRRLGEVFQTRIFEPLGMTDTTFELTDPM
                     RRRLAGIHARNADGSLTPMDFELPAKPEVHMGGHGLYGTIGDYMRFIRMWLNDGAGDH
                     GRVLKAETVRMAERNHLGDNKVTPITGVISALCNDAEFFPGQSKSWALTFMINDEEAP
                     TGRPAGALGWAGLANLFYWIDRRNGFGGFWATQILPFGDPASFIGYMNFETALYASLR
                     QRKAG"
     gene            complement(5982..7622)
                     /locus_tag="VN968_23075"
     CDS             complement(5982..7622)
                     /locus_tag="VN968_23075"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL32150.1"
                     /translation="MTEAATKSNGSNGAHATARFDAVVVGAGVAGLYQLYRLREQGLN
                     VMAIDAASAVGGTWYWNRYPGARFDSESFIYQYLFSEELYKGWSWSEKFPGQPEIERW
                     LNYVADRLDLRKNIQFGTTVKSAHFNEATQRWLITTDKGGVIDTQFLVTCCGMLSAPH
                     VSFPGQETFKGQLFHTARWPKQPVDFAGKRVGVIGNGATGIQAIQTIAGEVGHLKVFI
                     RTPQYIIPMKNPKYSAAEAEAYKSKFKRFTERLPHTFTGFEYDFEHAWAGLTRERRRE
                     VIEDCWNDGSLKLWISSFAELFFEEEVNAEISEFVREKMRERIKDPKLCEQLIPSEYG
                     FGTHRVPLEQNFLEAFHRPNVEIVGVKNNPIDCVTPTGIKTADGTVHDLDIIILATGF
                     DAGTGALTRIDIRGRGGRSLKDDWSRDIRTTMGLQVHGYPNLFTTAVPLAPSAALCNM
                     TTCLQQQVEWIDDCIGYMRRNSLEVVEPTKEAEDGWVAHHDEIANATLIAKTHSWYLG
                     SNVEGKPRRVLSYCGGVGAYRQKCDEVAAKDYQGFAMH"
     gene            7898..8143
                     /locus_tag="VN968_23080"
     CDS             7898..8143
                     /locus_tag="VN968_23080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32151.1"
                     /translation="MAKAAYSAGLPFGQREEAVNRAKELHGGDCAMGQFITVEELPRY
                     APGELMLDSLAVGKADFRLRVFRYAPSDIWVPPPRIF"
     gene            8173..8874
                     /locus_tag="VN968_23085"
     CDS             8173..8874
                     /locus_tag="VN968_23085"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXL32152.1"
                     /translation="MNRRVTGAWKQDHVGRGITTLLTRAEASNWRWSHDIEVSHFYIS
                     PTLMAKTASEAFDRDAEAVELHDLLRIDDPVLTWISDQMVQEVAAGGPGGRLCYDALT
                     LQASVHILRKYASVQFKMPCSQGRFRPAHARLIEDYIEENVSRNITLEELANICNCTP
                     VQFARKFHVHYGMRPHAYVLKRKVERACQHLRRDRLALKEIALLSGFSDQSHLNRVFR
                     RHMNLTPVEYRKQSA"
     gene            complement(9112..10314)
                     /locus_tag="VN968_23090"
     CDS             complement(9112..10314)
                     /locus_tag="VN968_23090"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HXL32153.1"
                     /translation="MRNHPIIVQKYGGVCLETPAKIRAVARSLADLHGRGHRVVAIVS
                     AMGKTTDQLIQMAYQVSPTPNRRELDMLLTTGERISMSLMSMALSDLGVPAISFTGSQ
                     AGVITDDAHSSARILDVRPIRVLEELDRGRVVVLAGFQGVNPVTREITTLGRGGSDTT
                     AVAMAAALKAERCEIIKDVDGICSADPRIVPNAKPLRRVDFASLSEMCFWGAKILHFR
                     SLELAQSQDVPLILKQWGGVKHGTQVMKEVAGMENGKVLAVNSMARIEHVEIDSKDLN
                     HGFEKFAQHLKQNSLSWPQLLASSFTVGKTSMTVACDSEWLDALLRTLERSKDLRKQR
                     EASSSVSLTCFGGVSSELPFRALQVLGHHGIVPDKYVLSPNSVSLFVPVENREAAVRA
                     LHSLVQQT"
     gene            complement(10315..11520)
                     /gene="nagA"
                     /locus_tag="VN968_23095"
     CDS             complement(10315..11520)
                     /gene="nagA"
                     /locus_tag="VN968_23095"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HXL32154.1"
                     /translation="MTAILGSILMGHAGSSFTIAAQHIFDGADMRGPGSVRISHGRIE
                     STTFGEAEARASIHPPADAILAPGFIDIQVNGGGGVLLNDQPTEAGVRRIVETHRKEG
                     TTGCLPTLITDRSEVIERLAAVAQACLKIPGVLGFHLEGPALNRSRKGIHPEAEIRVP
                     DRRDLAAIKSFGDRGRSIVTLAPECVPASMIDELIGAGLRIAAGHSDATAAEIGQAVD
                     RGVLGVTHLFNAMSQLNAREPGLVGAALEDGRLFAGIICDGIHVDRAGLRVAFRCKGR
                     DRLMLVTDAMPLVGTNDRQFMLQGRQITLHENRLTGPDGTLAGAHLTMIEAVRNAVAL
                     LGISLVDALIMASRTPAAFLGLESELGRIAPGYRADLVAFNPNFEIVGTWIGGVGSMG
                     EASEAFHRG"
     gene            11611..12534
                     /locus_tag="VN968_23100"
     CDS             11611..12534
                     /locus_tag="VN968_23100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HXL32155.1"
                     /translation="MGTKGTTYLGIDGGGTRCRARIEDENGRVLGAASSGPATTRIGF
                     EKAWRSIMEAAEATAAQAGLTREDFARMHAGIGLAGLGRRGAEAALNKIAHPFASVIF
                     ISDGLAACLGAHSGADGAIVVAGTGSVGVGLIDGREIRLAGYGFPVSDEGSGADIGLQ
                     VVRLALRAADRRGELTPLLSEVLGAFDHDPYQAVAWSEEARATDYAAFAPIVMRHANQ
                     GDPVGRRIVERAADAIGDLLDLFLARGIDRLSLVGGLADAITPWLTPDLRARLRCPDA
                     DAAAGALLVARGRLDLPKRETDHEQASKFHV"
     gene            12554..>12734
                     /locus_tag="VN968_23105"
     CDS             12554..>12734
                     /locus_tag="VN968_23105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HXL32156.1"
                     /translation="MATEEVDPRFADLDAWSLTSAMEAMWEGQLAAVAAIGHALPAIT
                     AATEAAKAALGDHGRI"
BASE COUNT         2458 a         3946 c         3877 g         2453 t
ORIGIN      
        1 cagcaccacc agcttctcgg cgcgcttgag cagcttggtc tcggcgcggg cgatcaaggg
       61 atcgccctcg atcacgccga gcggaccgat cgaaatcgcg ctcatgaaca tgcgcgaggc
      121 cgaataatgc tggatcgcgt cctcctcgaa cggtgaaacg atcagtttct gttcgcggta
      181 gacctcgccg cccggcagcg cgacgcgaca gttcgaatgg tggatcaacg tctcggccaa
      241 tggataggaa ttggtgagca ccttgaggcg gcgctcgcgc aggaattcgc ccatctcgaa
      301 ggtcgtggtg ccgccattga tgattatggc gtcgccgtcg ctgcacagcg cgaccgcggc
      361 ccgggcaatc gcccgcttgg cagcgacatt gagtgtcctg ctgatctcga aggagcgggt
      421 cgccagcgcc ggcgcgtcgc tcgaggccat ctgcgaacca ggcatcgcct ccaatccgcc
      481 gcgcacccgc gtcgccgaac ccatttcggc gagccgggca aaatcgcgtc gcaccgaagc
      541 ttcggaggcg ccggtggcgc ggcaaacgtc ggcgatacga accagcagct gctgcttcag
      601 catcgccctg atgacctgcc agcgctcgcg ttcgtgcacg gccttgttcc tcccaacgct
      661 cataactagt cacgaacttt ttgggtggtc aacgcgaaac cttcatattc aatcaatttc
      721 gcactgcatc aatcatatcg cgtggcaaga tcgttcacat cgtgattgac cgtgatcggt
      781 cgcgatccta tggtccgaaa aaagcgcatc atgacggaga cgaggctcat gaccaaggct
      841 gcggccgagc cgcttcccga tctttgggac gatgcccacg cggcggacct ggatgagcca
      901 ggcttgttgc tctaccgctc caatctgctc ggcagtgatc ttcgcaccac caatttcggc
      961 ggcggcaaca cctcggccaa gctcacaatg accgatccgc tgacccaaaa accaatcaac
     1021 gtgctgtggg tcaagggctc cggcggcgac ctcggctcga tgaagcgtga cggcttctcc
     1081 accctctatc tcgacaagtt ggagagcctg aaacagctct atcgcggcat cgtccatgaa
     1141 gacgagatgg tggcgctgtt caatcactgc accttcaatc tcaacccgcg cgcgacctcc
     1201 atcgacacgg ccttgcacgg cttcgtgccg cacccccatg tcgatcatgt ccatgctgat
     1261 gcggtgatcg ccgtcgccgc ctgctccaac gccgaggaac tgacgggaga agtgttcggc
     1321 ggcgcgatcg ggttcctgcc ctggcagcgg ccgggcttcg accttggcct gaagctcggc
     1381 gcgatggcgg cccgacatcg aaggcggcct accagaccac gttgcgcact atccagcagg
     1441 ccgccgactg gctcgctgga cacgaaaagc agcccgcctt cggcggtccg cgctgtgatg
     1501 ctgccgcgcc tgagacgcgc cgcgccgtcg cggcggcgct gatgccactg atccgaggca
     1561 agatctcgcg cgacgagcgc aaggtcgggc atttcaccga cgcgccggaa gtgctggagt
     1621 ttgtcaattc gcgcatgctg gacagactgg cgccgttggg aacatcttgt cccgatcatt
     1681 tcctgcgcac caaaatccgt cccctggtgc tgccctacga tcgccagagc gacagtgtcg
     1741 agacactcgc caattcgctc gacgcgcttc ttgaggccta tcgagacgac tattccgcct
     1801 attaccagcg ctgcaagcgg ccgaattcac cggggatgcg cgaccccaat gcggtgatct
     1861 acctggtgcc tggcatcggc atgttctcct tcgccaagga caaggcgacg gcgcgcatcg
     1921 cttcggaatt ctacgtcaac gccatcaacg tcatgcgcgg cgcctccggc gtcagcgtct
     1981 atgtcggcct gccggaacag gaagccttcg atatcgaata ttggctactc gaagaggcca
     2041 agctgcagcg catgccgaag gcgaaatcgc tggccggcag gatcgcctat gtcaccggcg
     2101 gcgccggcgg catcggcgga gcagcggcgt tgcgattatt gggcgaaggc gcctgtgtgg
     2161 tgctggccga catagaccag gaggcgcttg acgcgcgcat ccaggcgatc gtgcaaaaat
     2221 tcggccgcga cagcgtagtc ggcatcgcgc tggacgtgac cgacgaggcg agcgttgtcg
     2281 cgagcttcca cgagtccatt ctcgcctatg gcgggatcga tattgtggtc tccaacgccg
     2341 gcatcgcctc cgcctcgccg gtcgaggaca ccagcctggc actttggcaa aagaacatgg
     2401 atgtactggc caccggctat tttctggtgg cgcgcgaggc gttccggctg atgcaacggc
     2461 agcaatgcgg aggcgcgatc gtgttcgtcg cctccaagaa cggcctcgcc gcctcggccg
     2521 gcgcgtcggc ctactgcgcg gccaaggcct cggagattca tctggcgcgc tgcctcgcgc
     2581 tggaaggcgc gccgcacggc atccgcgtca acaccgtcaa tccggatgcg gtgctgcgcg
     2641 gctcgaaaat ctgggccggc gaatggcgcc agcagcgagc cgcctccaac aaggtgtcgg
     2701 aagacgaact cgaagaggtc tatcggcagc gatcgatgct gaagctttcg gtgttgccgg
     2761 aggacatcgc cgaggggatc tatttcttcg ccagcgatct ttcggccaaa tcgaccggca
     2821 atatcctcaa tgtcgatgcc ggcaacgcgc aggctttcac gcggtgagac gccaatcgtc
     2881 gggagacaat gatgaccgac ttctccatca gcgacgatct tctccaggct gagaacgccg
     2941 cgcgaaaacc ggcgctcgac gaggattacg ccgcattgtt gcgcgcgctg ggtcgccggg
     3001 gcattgacgg tgatgcgcta acacgaaggg tcatggcgtt cggcgtcgcg atcccgacct
     3061 ggggtgtcgg cacaggtggc acccgctttg cacgctttcc cggccccggc gagccgcgcc
     3121 atctgttcga caagctggag gattgcgcca ccatccacca tttggtgcgg gcaacgccga
     3181 cggtatcgcc gcattttcca tgggacgcga cgagtgacta cgctgccttg cgggaagtcg
     3241 ccgccaagca cggactagcg ttcgacgccg tgaactcgaa tacatttcag gatcagccga
     3301 accaggagca ttcatacaag ttcggctcgc tctcccatgt caaacccgcg gtgcgagccc
     3361 aagcgatcgc acacaacatc gattgcatcg ccatcggccg ccagctcggc tcagaggcgc
     3421 tgacagtgtg gatcggcgac ggctccaatt tcgccggcca atcgaacatg acgttggcgc
     3481 tcgatcgcta tctcgattca atgcgcgaga tctacgcggc gctgcccgcg gattggcggg
     3541 tgtttctcga gcataagctg tacgagccgg cgttctattc aagcgtgatt tccgattggg
     3601 gaaccagctt tgcggcggcg caggagcttg gaccgaaggc gcaatgcctg gtcgacctcg
     3661 gccaccatgc gccgaacgtc aatatcgaac agattgtcgc ccgcctcgtg cgctttggaa
     3721 aactcggcgg ctttcacttc aacgattcga aatacggcga cgacgatctt gacaccggat
     3781 cggtcgaacc gtttcgcctg tttctggtgt ttaacgaact gattgaggct cggatgcgcg
     3841 gtgcagaggg cttcgccccg gcctatatgc tcgaccaatc gcacaacgta accgatccga
     3901 tcgagagttt gatgcaatcg gcgatcgaat tgcagcgcgc tcatgcccag gcgctgatcg
     3961 tcgaccgctc agcgctggcc gcggcgcaag cggccaacga cgcgctcggc gcccacctcg
     4021 aactcaagcg cgcctacacc gccgacgtct cgccgatcct ggccatggca cggctgcggg
     4081 caggcggcgc catcgcgccg atcgagacct atcgcgcctc aggttatcgc aggcacaagg
     4141 ccaaggagcg gccaccggtg gcgaggacat cggcggggat cgtctgaggg aagcaagctc
     4201 cacgcacggc tccgcgatgc ctcaagcgag agacgccgcc gtccgagcgc cgtccgttaa
     4261 cccgacggca acgcatagcg aagcatccgg acttccccgc tccctagccg gggttcgcag
     4321 attcgcgctc gaaaatgcgg atttcgtagc gaagaccttt tgaactagcc cattgatttc
     4381 taacaagcgg gtatctctca aacccgccaa tccgcgaggg cgggtagccc aacttgcgca
     4441 gttggagata ttttcttgtg atcgttgccg acgcggccgg aacatgcatc aaaattggtc
     4501 agttctgtcc gtaagtggca gtggtgctcc cggtctgcgg atgcaacggc aagacctaca
     4561 acaatgactg tgagcgacaa aaacaggctg gagtgggtcg tatccttcat tagccgggac
     4621 atcatgcgga tgtgtccgac tcggcattgt ctgacgatcg aagttggaga ccggcctcaa
     4681 tcgagaggaa ggagaccggt cgcgctaagc gagagtttca atctcggccc atcaaactct
     4741 agcccgcctt tcgctgcctc aggctcgcgt acaacgcagt ctcaaaattc atgtaaccga
     4801 tgaacgacgc cggatcgccg aagggaagaa tctgggtggc ccagaacccg ccgaacccgt
     4861 ttcgccgatc gatccagtag aacaggttgg caagtccggc ccaaccgagc gcgccggccg
     4921 gacgtcccgt aggcgcctct tcgtcgttga tcatgaaggt cagcgcccag gatttcgact
     4981 gacccgggaa gaattccgcg tcgttgcaca gcgcggagat gacacccgtg atgggcgtca
     5041 ccttgttgtc acccagatgg ttcctttccg ccatgcgcac ggtctcggcc ttcagcacgc
     5101 ggccgtggtc gcccgcgcca tcattcagcc acatgcggat aaagcgcatg taatcgccga
     5161 tggtgccgta aaggccgtgg ccgcccatgt gtacttcggg cttggcaggc agctcgaaat
     5221 ccatcggcgt gagcgaacca tcggcattcc gcgcatggat cccggccagt ctccttcgca
     5281 ttggatcggt gagttcgaac gtcgtgtccg tcattcccag cggctcgaaa atgcgcgtct
     5341 ggaacacttc gccgagccgg cgatccgtga tggcctcgac gatttgaccg caccaatcga
     5401 gattggtccc gtattcccac cgctcgcccg gatcgaaaag cagcggcgtc atcaggcagg
     5461 ccttggaagc agtgatgacg ctgggctgtc ccttttcctg cgcgagtcgg ttataggtgt
     5521 ggttgatgaa atcgtaactc aggcctgagg tgtgaaccat cagcatgcgg gtggtcacgt
     5581 cgcgcttcgg tgcccgcagg atcggctcgc ccttggcgtc gaaaccgtcg attacctgga
     5641 gcttgccgat ctcgggcgca tagcgcctgg ccggcgcgtc gagatcaagc ttgccttctt
     5701 cgacgagttg caggacggcg gtaccggtga tcgccttggt cgtggaaaag atggcgaaga
     5761 tgctgtcggt cgtcatgccg gccggctgat cgagacggcg cttgccggcc gcaccttcgt
     5821 agatgttgcg atggcggtcc gtcaccatcg ctacgacgcc gggcacgcgc ggagtggatg
     5881 taacgacacc gtcgagaacg gcgtctgccg cccaactgaa attcgtgctc atgatcttcc
     5941 tccctctgta ttggaatggt ttcgtcggcg cggcgcttcg ctcagtgcat ggcaaaaccc
     6001 tggtagtctt tcgccgcgac ctcgtcgcac ttctgacgat aggcaccaac accaccgcag
     6061 taggacagca cacggcgcgg cttgccctcg acgttcgagc ccagatacca ggaatgggtt
     6121 tttgcaatca gcgttgcatt ggcgatctca tcgtgatggg ccacccatcc gtcctccgcc
     6181 tcctttgtcg gttcgacgac ctcgaggcta tttcgacgca tatagccgat gcagtcgtcg
     6241 atccattcga cctgctgctg caggcatgtg gtcatgttgc agagcgcagc cgagggagcg
     6301 agcggcaccg cggtcgtgaa gagattcgga tagccgtgaa cctgcaggcc catcgtggtg
     6361 cggatatccc tgctccagtc atccttcaat gaccgcccgc cgcggcccct gatatcgatc
     6421 cgcgtcagcg caccggttcc ggcgtcgaat ccggtcgcca gaatgatgat gtcgaggtcg
     6481 tggaccgtgc cgtcggcggt cttgatgccc gtgggtgtca cgcagtcgat cggattgttc
     6541 ttgacgccga caatctcgac attgggacga tggaaggctt cgaggaagtt ctgctcgagc
     6601 gggacccggt gcgtcccgaa cccgtattcc gagggaatga gctgctcgca cagctttgga
     6661 tccttgatcc gttcgcgcat cttctcgcgc acgaattcgg agatttcggc attgacctcc
     6721 tcttcaaaga acagctcggc gaaggacgat atccacagtt tcagggagcc gtcgttccag
     6781 caatcctcga tcacctcgcg gcgccgctct cgcgtcagac cggcccaggc gtgctcgaag
     6841 tcgtattcaa atccggtaaa ggtatgcggc agccgctccg tgaaacgctt gaacttcgac
     6901 ttgtaggctt ccgcttccgc cgcgctgtat ttgggattct tcatcgggat gatgtattgc
     6961 ggcgtgcgga tgaacacctt caggtgaccg acctcgccgg cgatggtctg gatcgcctgg
     7021 atgccggttg cgccgttgcc gatgacgccg acgcgcttgc cggcgaagtc gaccggctgc
     7081 ttgggccagc gcgccgtgtg gaacagctgc cccttgaacg tctcttgtcc cgggaacgac
     7141 acgtgagggg ccgagagcat gccgcagcag gtgaccagga actgggtatc gatcacgccc
     7201 cctttgtcgg tcgtgatgag ccagcgctgc gtggcctcgt tgaaatgcgc gctcttcaca
     7261 gtggtgccga actggatgtt cttgcggaga tcgaggcgat cggccacata gttcagccag
     7321 cgttcgattt ccggctggcc ggggaatttc tcgctccagc tccatccctt gtagagctcc
     7381 tcggagaaaa gatactgata gatgaagctt tccgaatcga agcgcgcgcc cggatagcga
     7441 ttccagtacc aggtgccgcc gacggctgac gccgcgtcga tcgccatgac gttcaatccc
     7501 tgctcgcgca ggcggtaaag ctgatagagc ccggcgacgc cggctccgac aaccaccgca
     7561 tcgaatcttg ccgtcgcgtg cgcgccgttg cttccattgg acttggtcgc ggcttccgtc
     7621 atttccttcc ctcccaggtg tttgcaagtc gcaggcggcc ctccccaccc gtcgtgtaat
     7681 cggatggcgg tgcgtggcat caatgtcggt cccggcaagt cgatcgggcg gcttctcttg
     7741 gcccacggct cttgcgccgt tcctcccgat cttgctcgag acgcgagcga ggctacgggg
     7801 gagccggtgt tccgggcttg aacaaaaccg acaatccttt gaacggaacc gcaagccctc
     7861 gccgtaccgc tgagatccgg cagagttcgc ttggcaaatg gcaaaagcgg cttattctgc
     7921 gggcctgccg ttcggccaac gagaagaagc ggtcaaccgc gccaaagagt tgcacggagg
     7981 agactgcgcg atgggccaat tcatcacggt cgaggagctg ccacgctatg ctcccggcga
     8041 actgatgttg gacagtcttg cggtgggaaa ggccgatttc cggctgcggg tgttccgcta
     8101 tgcgccgtcc gacatctggg tcccccctcc gagaattttc tgatcgtcgt ctaccgggaa
     8161 ggtacgacgt cgatgaatcg ccgcgtcacc ggggcgtgga agcaggatca tgtcggccgt
     8221 ggtatcacca cgctactgac ccgcgcggaa gcctctaact ggcgatggag ccatgacatc
     8281 gaagtcagcc atttctacat ctcgccgacc cttatggcga agactgcgag cgaggctttc
     8341 gatcgcgacg cggaggccgt cgaactccac gacctgctca ggatcgacga tccggtcctg
     8401 acctggatca gcgatcaaat ggttcaggag gttgccgcgg gaggtccggg cgggcgcctc
     8461 tgctacgatg cgctgacgct tcaggccagc gtgcacatct tgcgaaaata tgcttccgtg
     8521 caattcaaaa tgccctgctc gcagggacgt ttcaggccgg cccatgcgag attgatcgag
     8581 gactatattg aggagaacgt ctccaggaac atcacgctcg aggaattggc caacatctgc
     8641 aactgcacgc cggttcagtt tgcgcgcaaa ttccatgtgc actacggcat gcggccgcat
     8701 gcctacgtct tgaaacgcaa ggtggaacgc gcgtgccagc atcttcgccg ggatcgcctg
     8761 gcgctgaagg aaatcgcgtt actgagcggg ttttcggatc agagccacct caatcgggtc
     8821 tttcgccgac acatgaacct cacgcctgtc gaatatcgca agcaatcggc atgagacagg
     8881 cctgaacgac aggacatcgc agggaagcga atgccaaaaa ggcttccgcc gttttctacc
     8941 gcaccagttt tcgcaccaga aacgacctca tcaaaaggca acgaatgcga tcgaacgcag
     9001 acgaaaccgc agtgaaatca acgaagctga tcgatattct ggccgctcat aacggtctgg
     9061 ttgcaggttc gagtcgacgt aagcggaagc gatccctacc gcttctcgaa cctatgtttg
     9121 ctggacaagc gaatgcaacg ccctaaccgc ggcctcccgg ttctccacag ggacaaacag
     9181 actgaccgaa ttcggcgaca acacgtattt gtcggggaca atcccgtgat gtccgagaac
     9241 ctgcaacgcc ctgaacggca attccgacga aacgccgccg aagcaggtga gactcaccga
     9301 gcttgaagcc tcgcgctgct tgcgcagatc cttgcttcgc tccagcgtgc gcaacagggc
     9361 gtcgagccac tctgaatcac aggccacggt catgctggtt tttcccacgg tgaagctcga
     9421 ggcgaggagc tgcggccagg acaggctgtt ttgcttaaga tgctgcgcga atttctcgaa
     9481 accgtgattg agatctttgg aatcgatttc cacatgctca atgcgagcca tcgagttgac
     9541 ggcgagaacc tttccgtttt ccatgcctgc aacctctttc atcacttgcg tgccgtgttt
     9601 aacgccaccc cactgcttga gaatcagagg cacatcctga ctttgcgcca gctctaggct
     9661 gcgaaaatgc aggatctttg caccccagaa gcacatttcg gacagggatg cgaagtccac
     9721 tcgccgcagg ggctttgcat ttggcacgat gcgcggatcg gccgaacaaa ttccgtcgac
     9781 gtctttgatg atttcacagc gttcggcctt cagcgccgcg gccatcgcaa cggccgtggt
     9841 gtcgctgccg ccccggccga gcgtggtgat ttctctggtc accgggttca cgccctgaaa
     9901 tcccgccagc accacaacac gcccacgatc aagttcctca agcacgcgaa ttggccgtac
     9961 atcgagaatg cgcgcggagg aatgggcgtc gtccgtaatc acgccggcct ggctgccggt
    10021 aaagctgatt gcgggaacac cgagatcgga aagcgccatg ctcatcaggg acatgctgat
    10081 gcgctcgccg gtcgttagca gcatgtcgag ttcacggcga ttgggagtcg ggctaacctg
    10141 ataagccatc tggatcaatt ggtcggtggt cttgcccatg gcggagacga tcgccacgac
    10201 acgatgacca cggccatgca ggtcggcgag actgcgcgca accgcgcgaa ttttcgccgg
    10261 tgtttcaaga cagacaccgc catatttttg cacgatgatg ggatggttgc gcatctaacc
    10321 tcggtgaaaa gcctcgctcg cctcgcccat cgaaccgaca ccgcctatcc atgtgccgac
    10381 gatttcaaag ttgggattga aggcgacaag gtccgcgcgg tatccgggcg cgatccgtcc
    10441 aagctcggac tcaaggccca gaaatgccgc gggcgtccga gaggccatga tgagggcatc
    10501 gacgagggaa attccgagca gcgcaacagc gttgcgcacc gcctcgatca tggtgaggtg
    10561 agcgcccgcg agtgtgccat cggggccagt caggcgattt tcgtgcaacg tgatctgcct
    10621 tccttgcaac atgaattgcc ggtcgttcgt gcctaccaat ggcatggcat cggtgactag
    10681 catcaatcga tcgcgtccct tgcagcggaa ggcaacacgc aaaccggcgc ggtcaacgtg
    10741 gatgccgtcg cagatgatcc ctgcaaacag ccggccatcc tcaagcgcgg cgcccaccaa
    10801 ccccggctcc cgggcgttta actgcgacat ggcattgaac agatgggtca cccccaagac
    10861 accgcgatcg accgcctgcc cgatttcggc tgcggtggcg tcgctgtggc cggccgcgat
    10921 acgcagtccg gcgccgatca attcatcgat cattgacgcg ggcacgcatt ccggggccag
    10981 ggtcacaatg gagcggccgc ggtccccaaa acttttaatt gcggcgagat cgcgccgatc
    11041 gggcacgcgg atctcggctt ccggatgaat gcccttgcga gacctgttga gggccggtcc
    11101 ttccagatga aagccgagca cgccgggaat cttcagacaa gcctgggcga ctgcagccaa
    11161 tcgctcgatg acctcgctgc ggtcggtgat gagggtcggc aagcagcccg tggtcccctc
    11221 tttgcggtgc gtctcaacga tgcgtcgaac gccagcctcc gttggctggt cgttgagaag
    11281 gacgcccccg cctccgttca cctggatgtc gatgaatccg ggtgcaagga tcgcatctgc
    11341 cggagggtgg attgaagcgc gcgcctccgc ctcgccgaag gtggtgcttt caatccgccc
    11401 gtgcgagatc ctgaccgacc ccggcccgcg catgtcggca ccatcaaaga tgtgctgcgc
    11461 ggcaatcgtg aaagaagaac cagcgtggcc catcagtatt gatccgagaa tggcagtcac
    11521 gaactcgtga ggcgagtccc gtacaagact ccatatgtac actaaggaga tttgggagga
    11581 cccggagcga aaccttcgcg ttaactttcc atgggaacaa aaggtaccac gtatctcggc
    11641 attgatggcg gcggaacccg ctgccgcgcc cggatcgagg acgaaaatgg cagagtactc
    11701 ggtgcagcga gctcggggcc cgccacgacg cggattggct tcgagaaggc ctggcggtcg
    11761 atcatggagg ccgctgaagc gacagccgca caggccggac taacgcgcga ggatttcgcg
    11821 cggatgcacg ccggaattgg cctggccggt cttggtcgcc ggggcgcgga agcggcgctc
    11881 aacaagatcg cgcatccctt tgcgtccgtc atcttcatca gcgacggcct ggcggcctgt
    11941 ctcggcgccc atagcggcgc ggacggggcc atcgtggtag ccggcacagg ctcggtgggt
    12001 gtcggcctga tcgacggccg cgaaatccgt ctcgccggtt acggctttcc cgtgtcggac
    12061 gaaggcagcg gtgccgacat cggcctgcag gttgttcgac tggcgctgcg ggccgcggat
    12121 cgccgcggcg agctgacccc attgctttcc gaggtgctgg gcgcattcga tcatgatcct
    12181 tatcaggcgg tggcttggtc tgaagaagcc agggccacag actacgctgc gttcgcgccg
    12241 atcgtgatgc ggcacgccaa tcagggcgat ccggtcggcc gtcgcattgt cgaacgcgcg
    12301 gccgacgcca ttggcgatct gcttgatctg ttcctagcga ggggaattga ccggctctcg
    12361 ctggtaggcg ggctcgcgga tgccattacg ccctggctga cgcctgacct gcgcgcccgc
    12421 ctgaggtgtc ctgacgccga cgcggccgcc ggcgcgttac tggtggcgcg agggcggctt
    12481 gacctgccca agagagaaac tgaccatgag caggcttcaa agttccacgt ttaatggcgc
    12541 atggatcaac tatatggcca ccgaagaagt cgatccccgc tttgccgatc ttgatgcatg
    12601 gtcgctgacg tcagcgatgg aagcgatgtg ggagggccag cttgcggcag tcgcggcgat
    12661 cggccatgcc cttcccgcga tcacagcggc gaccgaagcg gccaaagcgg cgctgggtga
    12721 tcacggacgc attg
//
LOCUS       DATNOI010000572         2543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1467189,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000572 DATNOI010000000
VERSION     DATNOI010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467189"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VN968_23110"
     CDS             complement(<1..551)
                     /locus_tag="VN968_23110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXL32157.1"
                     /translation="MMNLAMNEHRLTKPGPQNAALRDYDSVRRAIAFISEHWRAQPTI
                     ESMADAAGVTPDELHHLFRRWAGLTPKAFMQALTLDHAKGLLRDSASVLDAALDSGLS
                     GPGRLHDLFVTHEAMSPGEWKNGGAGMTLAYGFHPSPFGTAIVIASGRGLAGLAFADP
                     GEEQASLADMQRRWPRASYVEDR"
     gene            complement(606..1130)
                     /locus_tag="VN968_23115"
     CDS             complement(606..1130)
                     /locus_tag="VN968_23115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HXL32158.1"
                     /translation="MTAGNDFDNSEPAQPELFSALLTPHRSLNRAGFLVLMGFLSAVS
                     FVAGLAFLLMGAWPVLGFFGLDVLAIYWAFRINFRDARATEEIRVTPSELRVRRVSHR
                     GHVVEWVLNPLWVQLDQKCHAEFGIEKLYLVSSGRRVSVARFLGADEKASFAKALTAA
                     LQAAKRGPTYNPVV"
     gene            1153..1962
                     /gene="nth"
                     /locus_tag="VN968_23120"
     CDS             1153..1962
                     /gene="nth"
                     /locus_tag="VN968_23120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXL32159.1"
                     /translation="MSKIIRRPLTKSKPTAAKPAKKSRKRAAASQSRESAAKKAAAKS
                     GPTRAAKKSVRAPRLWTSAEVHEAFSRFRSANPEPKGELEHLNPYTLLVAVVLSAQAT
                     DAGVNRATRALFAVADTPQKMLALGEDNVRDYIKTIGLYRNKARNVIALSEKLIAEFG
                     GDVPRSRAEIESLPGAGRKTANVVLNMAYGEHTMAVDTHVFRVGNRTGFAPGNTPLEV
                     ELGLEKVIPAEFMLHAHHWLILHGRYTCLARKPRCELCLINDLCRWPEKTI"
     gene            2109..>2543
                     /locus_tag="VN968_23125"
     CDS             2109..>2543
                     /locus_tag="VN968_23125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HXL32160.1"
                     /translation="MNDKAALVSLATSVPPHLFHQKQILEAASKLMADRYPQFETMSS
                     LFANTGIRHRYGVKPMEWYLERRGWPERTRAFLEGAEALFVDVARKALAQAALSAGDI
                     DTVVTVCSTGIATPTLEARVAGQLGFRSDVSRVPVFGLGCAGG"
BASE COUNT          512 a          821 c          782 g          428 t
ORIGIN      
        1 tcgcgatctt ccacatagct cgcgcgcggc cagcgccgct gcatgtcggc aagcgaggcc
       61 tgctcctcgc cgggatcggc gaaagcaaga ccggcgaggc cccggccgct ggcgatcacg
      121 atggcggtgc cgaatggcga cgggtgaaat ccgtaagcca gcgtcatgcc ggcgccgccg
      181 ttcttccatt cgcccggcga catcgcctca tgagtcacga acaggtcgtg cagccgtccc
      241 gggcccgaaa gcccggagtc gagcgcggca tcgagcacgc tggcggaatc gcgcagcaag
      301 cccttggcgt gatcgagcgt cagcgcctgc atgaaggcct ttggcgtcag cccggcccag
      361 cggcgaaaca gatggtgcag ctcatccggc gtcacgccgg ccgcatccgc catcgattcg
      421 atggtcggtt gtgcgcgcca gtgctccgag atgaaggcga tggcgcggcg caccgaatca
      481 tagtcgcgca gcgcggcgtt ctgggggccc ggttttgtga ggcggtgctc attcatggcg
      541 aggttcatca tggatctgat gtaggctagg cgcggtgtcc aaaccacccg attcccgata
      601 gcggttcata ccaccggatt ataggtcggg ccacgcttgg cggcctgtag cgctgcggtt
      661 aaggctttgg caaagcttgc tttttcgtcg gcaccgagga atcgtgcgac cgaaacccgg
      721 cggccgctcg agaccagata aagcttttcg ataccgaatt ccgcgtggca tttctggtcg
      781 agctgcaccc acagcggatt gagcacccat tcgaccacgt ggccgcggtg gctcacccga
      841 cgcacacgaa gctccgacgg cgtcacccgg atttcctcgg tggccctggc gtcgcggaaa
      901 ttgatccgga atgcccagta gatcgccagc acatcgaggc cgaaaaagcc caacaccggc
      961 cacgcgccca tcagcagaaa cgccagcccc gcaacgaagc tgaccgcgct gagaaacccc
     1021 atcagcacca gaaaaccagc acggttcagc gagcggtgcg gcgtcagcag cgccgagaac
     1081 aattccggct gcgccggctc tgaattgtca aaatcgttgc ctgcggtcat cacaggtcag
     1141 tatacccgga tcatgtcgaa aatcatccgc agacctctga ccaagtcgaa gcccaccgcc
     1201 gctaaacccg ccaaaaaatc tcgcaaacgc gccgccgcaa gccagtcgcg cgaaagcgca
     1261 gcgaaaaagg ctgcggctaa atcgggtccg acgcgcgctg cgaaaaaatc ggtcagggct
     1321 cccaggctct ggacatcagc cgaggtgcac gaagccttca gccgttttcg cagcgccaat
     1381 cccgagccga agggcgagct cgagcatctc aacccctaca cgctgctggt cgcggtggtg
     1441 ctgtcggccc aggcgaccga cgccggcgtc aaccgggcga cgcgcgcgct gttcgcggtc
     1501 gccgacacgc cgcaaaaaat gctcgcgctc ggtgaagaca acgtacgcga ctacatcaag
     1561 accatcgggc tctaccgcaa caaggccagg aacgtcatcg cgctgtcgga aaaactgatt
     1621 gccgaattcg gcggcgacgt gccgcgcagc cgcgccgaga tcgaaagcct acccggcgcc
     1681 gggcgcaaga ccgccaatgt cgtgctcaac atggcctatg gcgagcacac catggcggtc
     1741 gatacccacg tgtttcgcgt cggcaatcga accggtttcg cgccgggcaa tacgccgctc
     1801 gaggtcgagc ttgggctgga gaaagtaatc ccggccgagt tcatgctgca tgcgcatcac
     1861 tggctgatcc tgcatggccg ctatacctgt ttggcccgca agccgcgctg cgaactatgc
     1921 ctgatcaacg atctctgccg ctggccggaa aagacgatct agctttttcg cgagcaccgt
     1981 ctctccgtca ttcaacttat agtgatgccc cggcctgccc ggcatcggcg gcatccgaaa
     2041 tattcgccac accgcgaggt tcatataacg aggtaccaga cgcagggtga gtgagcaggg
     2101 tgagtgatat gaatgacaag gccgcactgg tctcgcttgc gacctcggtt cccccgcatc
     2161 tgtttcatca gaaacaaatc ctggaagcag ccagcaagct gatggcggac cgctatccgc
     2221 agttcgaaac catgtcgagc ctgtttgcca acaccggaat acgccaccgc tacggcgtca
     2281 aaccgatgga atggtatctc gaacggcgcg gctggccgga gcgaacgcgg gcgtttctcg
     2341 aaggcgcgga ggcgctgttt gtcgacgtcg cgcgcaaggc gctggcgcag gcggctcttt
     2401 cggccggtga tatcgacacc gtcgtcacgg tatgttcgac cggcatcgca acgccgacgc
     2461 tggaagcgcg ggttgcgggc caacttggtt tccgcagcga cgtgtcgcgt gtgccggtat
     2521 tcggtctcgg atgcgccggc ggc
//
LOCUS       DATNOI010000573        12071 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2202268,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000573 DATNOI010000000
VERSION     DATNOI010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12071
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2202268"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VN968_23130"
     CDS             complement(<1..468)
                     /locus_tag="VN968_23130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXL32161.1"
                     /translation="MARPLSIAIIGAGMGGLAAAAALRRVGIDVTVYEQAAQFIRLGA
                     GIQIGCNAMKVLRGLGLESRLREQSFYPRSWNNRDWRTGEIKFDMIFGESAEKKFGAP
                     YLLAHRGDLHAALASIVPHEGVKLSHRLIGLEESNGGVRLAFANGAIAVADAVI"
     gene            complement(494..2443)
                     /locus_tag="VN968_23135"
     CDS             complement(494..2443)
                     /locus_tag="VN968_23135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HXL32162.1"
                     /translation="MSSAPSSNSPDGVDQDQFLNILSREEALERFEAALCPRPIPNEQ
                     RPLSDALGCALAQDIVAPIDVPPFDRANVDGFAVRSADLASATEATPVRVMLNDEVIA
                     CGTAPIRPVLSGTATSIATGGPVPRGADAIVMVEHTQPIANRAIEIRRAASPGQFISY
                     AGSDIARGEALLRAGTVIGSREIGMLAASGIAQVAVARRPRVAVVSTGDELVQPGQPL
                     RPAEIYDTNGAIVAAAVSENGGEAVFLGAIADDEKQLEATMRKGLETCDLLVLSGGTS
                     KGAGDVSHRIIARLGKPGIIAHGVALKPGKPLCLAVCDGKPVVILPGFPTSAMFTFHD
                     MIVPVLRRMAGLPPRSDARVMAKVPVRIASELGRTEFVMVSLVEGDRSLIAYPSGKSS
                     GAITSFAQTDGFLKIDALADQMAAGSDAEVTLFTPHVRVPDLVIIGSHCTGLDLVAAP
                     LARAGFVVRSIAVGSLGGLAAARRAECDFAPIHLFDEATESYNVPFLMAGLELVPGWR
                     RMQGIVFRVGDARFEGSNVERAVRAALADSACIMVNRNQGAGTRILIDRLLGDARPEG
                     YWNQPRSHNAVAAAVAQHRADWGVTIAPVAHASGLGFIPLTEEHYDFALVTARKQRPA
                     VQAFLRALTSEESRAALAQAGFRPA"
     gene            complement(2457..3578)
                     /locus_tag="VN968_23140"
     CDS             complement(2457..3578)
                     /locus_tag="VN968_23140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HXL32163.1"
                     /translation="MIPTQRLPPSLTPLEEALAALLDGLKPVAPIGLSLADALGCVAA
                     DMPPLKAFPPADIAAGDGWALRARDLIGASSYSPLPLARLPVWVEAGDAMPEGCDCVI
                     DSDLVDQAGPMVQVLAEAIPGQGVRRIGGDIAQAGSVIAAGRRIRPLDLLVARAAGLE
                     RLNVRRPRLRAVAIPATSGHAATAQLISENARAAGANVVLVEAKGRDGESIVTTLDSA
                     SCDVLVTIGGSGVGRSDAAIAALARRGEVLAHGIALQPGRTAAVGRIGKVPVIALPGA
                     PDQALAAWWALALPVLNRLSGRLARQPVTLPLARKIASSVGIAEIVLLERKEGDWMPL
                     SVGDMSLQTIARADGWFAVPAASEGFAAGTPVDAYMLRD"
     gene            complement(3616..4413)
                     /locus_tag="VN968_23145"
                     /pseudo
     CDS             complement(3616..4413)
                     /locus_tag="VN968_23145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
     gene            complement(4478..6514)
                     /locus_tag="VN968_23150"
     CDS             complement(4478..6514)
                     /locus_tag="VN968_23150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXL32164.1"
                     /translation="MSVDSSHLLICSCERTMPLDAQSIGRGCAGKITQANQLCGHELE
                     RFKQALADGTPVTVACTQEAPLFREVAENFPQAQLTFVNIRETGGWSNDAAAAGPKAA
                     ALIAAAGEEMPPITLVTLESTGVALIYGRDEIAVEAAQRLADRLDITVLLTKPGDVTP
                     RRTNEFPVLKGTVRNARGHLGRFELAIDDYALPSPSSRARLVFGPSRDGATSTCDLIL
                     DLSGGTPLFPAHQLRPGYLRADPRDRAAVERAIADAGGLVGTFDKPRFVHFEEALCAH
                     SRSGITGCTRCLDLCPTGAITPNGNAVSIDENVCAGCGSCASVCPTGAASYSLPSADA
                     LMRRLRTLLQTYRKAGGSEAIVLFHDGEHGEAIIDALARFGAGLPANVLPLRVNEVTQ
                     LGPEVVAAVFAYGGSGAAMITRAKPRHDISGLRRTVEMSDGIISALGFGAGLVRILET
                     DDPDQLRAMLDAAPVGVATQKPAGFVPRGAKRGVLETTFRELHLAAPAPVDVVPLAAG
                     APFGGVNLNVEGCTLCHACVTACPTGALSDNPDRAMLRFTESLCVQCGLCESTCPEKV
                     ISLEPRLDFKAWNAPLAVLKEEEPFLCIACGKPFGTKSSIERVLAKLGEKHWMFQGAN
                     AKRLDVIKMCADCRVEAVVNESFDPHAAPQRPPVLTTEDYLRARDAKKGDPLGS"
     gene            6663..7418
                     /locus_tag="VN968_23155"
     CDS             6663..7418
                     /locus_tag="VN968_23155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate--protein ligase family protein"
                     /protein_id="HXL32165.1"
                     /translation="MASRPADVEQTLDLPPGYTLVALREFGDAFVHGCAIASDIGAGT
                     LVWVRRYDLVEFAVVLEPEEPLLSARRAFFAGMNAAADAIAAHCPPEREVSFDWPDAI
                     RFDGGLLGGARLGWPADCAEDEVPAWLVFGVMLRAADMAHVEQVQTATGVSLLSEGFE
                     MVDTDAIIGSFARHLMTAFDLWNERGFETIARDYLARLPSRKAAERRRIDLSGDLLIT
                     APTGSGPPERASLVDALVKAAWYDPQARGPKLG"
     gene            7426..7998
                     /locus_tag="VN968_23160"
     CDS             7426..7998
                     /locus_tag="VN968_23160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6505 family protein"
                     /protein_id="HXL32166.1"
                     /translation="MLRFPRTIRLDPSDTFVFERAAEPGEWAVSGAFVFWNQDPANLG
                     QKQRVALRSGFLGIDSLGWSTLAIVTEATEADRQAMVERLASQLQKKFGAPDLNAARL
                     AAEEEVAFAASLCDHPPQTLLAVQRSVENGEIRERFRTLKPREAAAGADRLHAHAPAF
                     TFHEVEGDVEPTEEVDLPGMIRNDRRTDRT"
     gene            7995..9014
                     /locus_tag="VN968_23165"
     CDS             7995..9014
                     /locus_tag="VN968_23165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HXL32167.1"
                     /translation="MREFWVASGHHLTHRAEHGGLVATPELITAYLARPELMPPADAC
                     DAERNLHASLLAEPLRPVSRADIAALADADARENWTFMVNFRDRLISAPSLEALYTTL
                     ARKGSGELPPIFLSQLCHLILRNALEGCDDPYTLRAGELFYRSQLAAIHDGALLLADA
                     EVIEAQQHTQHDLHSSPLTALLQPKPFAEMDVMDDENAWTYWSRSDAHAMVMNIGGNA
                     KARAGLCRAIERWISHLLGIAVQVETVASIEDRDWRWFVGLDSEATRIGNALWNGAAL
                     DGNASERIVALMRLDIEDARLVDERVGNKPIYLILAMGADRTVRLKPQNLIAGLPIKH
                     LEAAS"
     gene            9011..9526
                     /locus_tag="VN968_23170"
     CDS             9011..9526
                     /locus_tag="VN968_23170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HXL32168.1"
                     /translation="MSPAAVPLLRIPVGVVVERRKTDSPWIDFIWRGVGVLPDEPEMK
                     PWTVLREQDEATLYYAGSATVDLYRSETQLYRDNIASGTPSIWIVLSPSEGSWPYAIS
                     AVTADPAEGEAFTEAAANLVEAVAMPEMLRDAIENFIAEHHVEREFVKRERRRADPEA
                     LARRQHEGGRK"
     gene            9523..10197
                     /locus_tag="VN968_23175"
     CDS             9523..10197
                     /locus_tag="VN968_23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HXL32169.1"
                     /translation="MSDDEFLARWSRRKQEARSGNPAPEPAEPMQAHEPTPPSGTAEI
                     PPNPDPDLSSLPPIESIDAATDITAFLRKGIPQELSRAALRRAWTADPAIRDFVGLAE
                     NAWDFNDPNAMPGFGPLDCSAEQLKALADRVVGGVRQVTEKLADSLEEAENMRRLAAP
                     EHDPALDEQKSNPVGTIAGLPATDQSMSPDVSPAPAAPQPETAERAESGETPVRHRTH
                     GGALPR"
     gene            10320..11096
                     /locus_tag="VN968_23180"
     CDS             10320..11096
                     /locus_tag="VN968_23180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HXL32170.1"
                     /translation="MRDPVLNRAIDAAGGVVQLARKIGIAQPSISNWNRVPAERVIAV
                     EAATGVSRQQLRPDLYSEPAVKDDAVDPVDAARAQEYALLAALLSSPPSNELLGEIAR
                     LRGDATPLGRAHAALAEAASEATAEVDREYFDLFVGLGRGELLPYGSYYLTGFLNERP
                     LSRLRDDLAALGIERVANNFEPEDHAGTLCEIMAGLAVGRFPASAEMQRRFFEKHVAP
                     WMGRLFADMETATSAKFYRSVGSLGRLFLKIETEAFTFAN"
     gene            11126..11335
                     /locus_tag="VN968_23185"
     CDS             11126..11335
                     /locus_tag="VN968_23185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HXL32171.1"
                     /translation="MRDEKKTKVGRRDFLRKVGVGAAGAGATLATPLMGTAQADSEND
                     DEKRKPRYKETDHVKAYYRVNRYPA"
     gene            11346..>12071
                     /locus_tag="VN968_23190"
     CDS             11346..>12071
                     /locus_tag="VN968_23190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXL32172.1"
                     /translation="MLIKRTQSQRFSSDRRGSVAGALSGHTGGLDRRAFLRRAGLAGG
                     ALAALGTLPLGSVRKAEAAVAGPLSAGAAVRKSICTHCAVGCTVTAEVLNGVWVGQEP
                     SWDSPINRGSHCAKGASVRELVHSVRRLRYPMKLVNGQWTRLSWDTAINEIGDKLLQI
                     REKSGGDSVYWLGSAKMTNEGAYLFRKFGAFWGTNNTDHQARICHSTTVTGVANTWGY
                     GAMTNSFNDIRNAKTQIIMGGNPA"
BASE COUNT         2190 a         3934 c         3903 g         2044 t
ORIGIN      
        1 gatgaccgca tccgcaaccg cgattgcgcc attggcaaaa gccaaccgga cgccgccgtt
       61 gctctcctcc agtccgatca gccggtggct cagcttgacg ccttcgtgag gaactatgct
      121 ggccagggcg gcgtgcaggt ctccacgatg ggcaagcaaa tagggcgcgc caaatttctt
      181 ttcggcgctc tcaccgaaaa tcatgtcgaa tttgatttcg ccggttcgcc agtcccggtt
      241 gttccaggag cgcgggtaga aggactgttc gcgcaatcgc gattcgagcc ccagccctcg
      301 cagaaccttc atggcgttgc agccgatctg aattccggcg cccagcctga tgaattgtgc
      361 agcctgctcg tacaccgtga catcgatacc cacccgcctc agggccgccg cggcagcgag
      421 cccgcccatg ccggcgccga taatcgcaat tgataaaggt cttgccatcg atgtccgcca
      481 gggtgcacgc cggctaagcc ggcctgaagc cggcctgcgc cagcgcggcc cgactttcct
      541 cggatgtcag agcccgcaaa aacgcctgca ccgcgggacg ttgtttgcgc gcggtgacca
      601 gcgcgaaatc ataatgctct tcggtgagcg gaatgaagcc caggcccgac gcatgcgcga
      661 ccggcgcgat ggtcacaccc cagtcggcgc gatgctgcgc gacagcggct gcgaccgcgt
      721 tatgcgagcg cggctgattc cagtagccct ccgggcgcgc gtctccgagc agccggtcga
      781 tcaggatgcg cgtgcctgca ccctggttgc ggttgaccat gatgcaggcg gagtcggcga
      841 gcgcggcgcg caccgccctt tccacattcg acccctcgaa ccttgcatcg ccgacacgaa
      901 atacgatgcc ctgcatccgc cgccagcccg gcaccagttc gagaccggcc atcagaaacg
      961 gcacgttgta gctttcggtc gcttcgtcaa acagatggat cggcgcgaaa tcgcattcgg
     1021 ctcgccgcgc cgccgcgaga ccaccgaggc tgccgaccgc gatcgagcgc acgacgaaac
     1081 cggcgcgcgc cagcggcgca gctacaagat cgagcccggt gcagtggctt ccgatgatga
     1141 cgagatcggg cacccgcaca tgcggcgtga acagcgtcac ctcggcatcg gaaccggccg
     1201 ccatctgatc ggcgagcgca tcgatcttca aaaaaccgtc agtctgcgcg aacgacgtga
     1261 tcgcgccgga gctcttgccg gagggatagg cgatcaggct tcgatcgccc tccaccagcg
     1321 acaccatgac gaattcggtg cggccgagtt cggaggcgat ccgcaccggg acctttgcca
     1381 tgaccctggc atcggagcgc ggcggcagtc ccgccatccg ccgcagcacc ggcacgatca
     1441 tgtcgtgaaa ggtgaacatc gcggaggtcg ggaatcccgg caggatcacc accggcttgc
     1501 cgtcgcagac cgcaagacaa agcggtttgc cgggtttcag tgcgacgcca tgcgcgatga
     1561 tgccgggttt tccaagccgc gcgatgatgc gatgggacac gtctccggcg cccttcgagg
     1621 tgccgccgga tagcaccagc aagtcgcagg tctcaagccc cttgcgcatg gtggcttcga
     1681 gttgtttttc gtcgtcggca atggcgccga gaaacactgc ctcgccgccg ttctcgctca
     1741 ccgccgccgc gacgatcgcg ccgttggtgt cgtaaatctc ggcggggcgc aacggctggc
     1801 ccggctgcac cagctcgtcg ccggtggaga ccaccgcgac gcgcggccgg cgcgctacgg
     1861 cgacttgcgc aatcccgctt gcagccaaca tgccgatctc gcgcgagccg atcacggtgc
     1921 cggcgcgcaa cagcgcctcg ccgcgcgcaa tgtcggagcc cgcataggat atgaattgtc
     1981 ccggcgaggc ggctcggcgg atctcgatcg cgcgattggc aatgggctgg gtgtgctcga
     2041 ccatgacaat agcatcggcg ccgcgcggca ccggaccgcc ggtcgcgatc gaggtggcgg
     2101 tgccggacag caccggccgt atcggcgcgg tgccgcaggc gatgacttcg tcattcagca
     2161 tgacgcgcac cggtgtcgcc tcagtggcgg aggcgagatc ggcggagcgc accgcaaagc
     2221 cgtcgacatt ggcgcggtcg aacggcggca catcaatcgg cgccacgata tcctgcgcca
     2281 aggcgcagcc gagcgcgtcc gaaagcggcc gctgctcgtt cgggatcgga cgcggacaca
     2341 gcgcggcttc gaaacgctca agcgcctctt cgcgcgagag gatgttcagg aactggtcct
     2401 gatccacgcc gtcggggcta ttcgaagagg gcgcactgct catgtcggaa cccatatcag
     2461 tcgcgcagca tataagcatc caccggcgtg cccgcagcaa aaccttcgct ggctgcgggc
     2521 accgcgaacc agccatcggc acgcgcaatg gtctggagcg acatatcgcc gacagaaagc
     2581 ggcatccagt cgccctcctt tctttcgagc aggacgattt cagcaatgcc gacgcttgag
     2641 gcaatcttgc gcgccagcgg caacgtcact ggctgccgcg ccagccgtcc cgaaagccgg
     2701 ttcagcacgg gcagcgccag cgcccaccat gccgcgagcg cctgatccgg tgcccccggc
     2761 aacgcaatga ccggaacctt tccaattctg ccgacggcag cagtacgtcc cggctgcaac
     2821 gcgatgccat gggcgagaac ctcgccgcgc cgggccagcg ccgcgatcgc cgcatcgctg
     2881 cggccgacgc cgctgccgcc gatagtgacc agcacatcgc acgatgcgct atcgagcgtc
     2941 gttacgatcg attcgccatc gcgccctttg gcctcgacga ggacaacgtt cgcacccgct
     3001 gcgcgcgcgt tctccgaaat cagctgcgcc gtcgccgcat ggcctgaggt cgcgggaatg
     3061 gcgacggcgc gcaggcgcgg ccggcgcacg ttcaatctct ccagacccgc cgcgcgcgcg
     3121 accaaaaggt ccagcgggcg aatgcgccgc ccggcggcaa tgacggaacc agcttgcgcg
     3181 atgtcgccgc cgatccggcg aacgccctgc cctggtatcg cttcggccaa cacctgaacc
     3241 atggggcctg cttgatcgac gagatcagaa tcgatcacgc aatcgcaacc ctccggcatg
     3301 gcatcgccgg cttcgaccca gaccggcagt cgggccagcg gcagcggcga ataggacgac
     3361 gcgccgatga gatcgcgggc acgcaatgcc cagccatcgc cagcggcgat atcggccggc
     3421 ggaaacgcct tcaatggtgg catgtcggcg gcgacgcagc cgagcgcatc ggcaagcgaa
     3481 agcccaatgg gcgccaccgg cttcaagcca tccagcaaag ccgcgagagc ctcttcgagc
     3541 ggggtaagcg aaggcggcag gcgctgggtc gggatcataa ggggctctcc tcgcccgact
     3601 gtgcggccca cgtgctcact cgatgacaat gcggggcgcg ggttttgtcg cgctacctgc
     3661 ggtgattgcc gaccagacgt tgcgggcgat gtcgatgtaa tgcctcgcat gaacgccgtc
     3721 gggctcggtc gcgacaatgg ggcgtccgtc gtcggaggtt tcgcggatcg cgatatcgag
     3781 cggtatctcg ccgagaaatg gaattccgcg catttcggcc tcgtgccgtg cgccaccgtg
     3841 gccgaatatt ggcgtgacct gatggcaggc cgggcagcaa aagcccgaca tgttttcgat
     3901 caatcccaac agcggcacgt ttactttctc aaacatcgcg atgccccgcc gcgcgtcgat
     3961 cagggcgatg tcctgcggcg tcgagacaat caccgcgccg gtgagcggcg tttgctgcgc
     4021 catggtgagc tgggcatcgc cggtgcccgg cggcagatcg acgacaagaa tatcgagctc
     4081 gccccacgcc acctcgcgca gcatttgcgt gatcgccgaa atcaccatcg gtccgcgcca
     4141 gatcatggcg aggtcttcct cgaccagaaa gccgatcgac atcaccttga cgccaaaacg
     4201 ttccatcggt tccagcatcc gcggccccac cagacgcggt ttgccgcgca ggccgaacaa
     4261 tttcggctgg gaggggccat agacgtcggc atcaaggatg ccggttttca gtccgatcgc
     4321 ggcgaagccg agcgcgagat tgcacgaggt ggtcgacttg ccgacgcctc ccttggcgga
     4381 ggcaacggcg atggcgtgcc tgacgccggg aatgccggcc gctctcgagg tcgcgcgcgc
     4441 ttgcaaggct ggacgggtgg cggacacgat gggctcctca cgatccaagc ggatcgccct
     4501 tcttcgcgtc gcgcgcgcgc aaatagtcct cggtggtcaa gacgggaggc cgctgcggcg
     4561 ccgcgtgcgg gtcgaaactc tcgttgacca cggcttcgac ccggcaatcc gcacacatct
     4621 tgatgacatc gagacgcttg gcgttggcgc cctggaacat ccagtgcttc tcgccgagtt
     4681 ttgccagcac gcgctcgatc gagctcttgg tgccaaaggg cttgccgcag gcaatgcaga
     4741 gaaacggctc ttcctctttc agcacggcaa gcggcgcgtt ccaggccttg aaatcgagcc
     4801 gcggctcaag gctgatgacc ttttccggac aggtcgattc acaaagcccg cattgcacgc
     4861 acaggctttc ggtaaatcgc agcatcgcgc ggtcgggatt gtccgacagc gccccggtcg
     4921 gacaggcggt gacgcaggca tggcagagcg tgcaaccctc cacgttgaga ttgacgccgc
     4981 cgaacggcgc gcccgccgcc agcggcacga catcgaccgg cgcgggcgcc gccagatgca
     5041 gctcgcgaaa tgtggtctcg agcacgccgc gtttggcgcc gcgcgggaca aagccggccg
     5101 gcttttgcgt cgcgacgccg accggtgctg catcgagcat ggcgcgcaac tggtcgggat
     5161 cgtcggtctc gaggatccgc accagccccg cgccgaagcc gagcgccgaa atgatgccat
     5221 ccgacatctc gaccgtgcgg cgaagcccgg aaatatcgtg ccttggtttt gcgcgcgtta
     5281 tcatcgccgc tccactgccg ccataggcaa ataccgccgc gacgacctct ggtccgagct
     5341 gcgtcacctc gttgacgcgc agcggcagca cattcgctgg cagtccggcg ccgaaccgcg
     5401 cgagggcgtc gatgatcgcc tcgccgtgct cgccgtcgtg aaacaacacg atggcctcgc
     5461 tgccgccggc cttgcgatag gtctgcaaca gcgtgcgcag ccgccgcatc agcgcgtccg
     5521 cgctgggcag cgaataggat gccgcgccgg tcggacacac agatgcacac gagccgcagc
     5581 cggcgcagac gttttcgtcg attgagaccg cattgccgtt cggcgtgatc gcgccggtcg
     5641 gacacaggtc aagacagcgc gtgcatccgg tgatgccgga gcgcgaatgg gcgcagagcg
     5701 cctcttcgaa gtgaacaaag cgcggcttgt cgaaggtgcc gaccagaccg cctgcatccg
     5761 cgatcgcgcg ttcgacggcg gcgcggtcgc gtggatcggc gcgcagatat cccggacgca
     5821 attggtgcgc cgggaagagc ggggtaccgc ctgagagatc gaggatcaga tcgcaggtcg
     5881 aggttgctcc gtcgcgcgaa ggtccgaaca cgagcctggc gcgcgaggac ggcgatggca
     5941 gcgcgtagtc gtcgatcgca agctcaaaac ggccgagatg tccgcgcgcg ttgcggacgg
     6001 ttcctttgag aaccggaaac tcgttggttc tgcgcggcgt cacgtcgccg ggttttgtca
     6061 gaagcactgt gatgtcgagg cgatcggcga gccgctgggc ggcctcgacc gcgatctcgt
     6121 cgcggccata gatcagggcg acgccggtgc tctcgagcgt caccagcgta atcggcggca
     6181 tctcttcacc ggccgccgcg atcagcgccg cggctttcgg accggccgcc gcggcatcgt
     6241 ttgaccagcc gccggtttcg cggatgttca caaaggtgag ttgcgcttgc gggaaattct
     6301 ccgcgacttc cctgaacagc ggggcttcct gggtgcaggc gaccgtaacc ggcgtgccgt
     6361 cggcaagcgc ttgcttgaac cgctcgagct cgtggccgca gagttggttc gcctgggtga
     6421 tctttcccgc gcagccgcga ccgatcgact gcgcatcgag cggcatggtc ctctcgcagc
     6481 tgcaaatcag gagatgagac gagtccacac tcattaatga tttatcctga accgggttcc
     6541 gttctccggt tccgcggtac ctgctagagt gaaggtccgc aaggtccgga ccgctcgcgt
     6601 cgccaccttg atcgggatag catacacccg cgaattgcaa agtgcttaag gatgaatctt
     6661 cgatggcgtc gcgtcctgcc gacgtagagc agacactgga tctgccgcct ggttatactt
     6721 tggtcgcgct gcgcgaattt ggcgacgcct tcgttcacgg ctgcgcgatt gcttccgata
     6781 tcggcgcggg aacgctggtc tgggtgcgcc gctacgatct ggtcgaattc gccgtggtgc
     6841 tggagcccga ggagccgctg ctgtcggcgc gccgggcgtt tttcgccggg atgaatgcgg
     6901 cggcagatgc gattgccgcc cattgcccgc ccgagcggga agtcagtttc gactggcctg
     6961 acgccatccg gttcgatggc gggctgcttg gcggcgcgcg gctcggctgg ccggcggatt
     7021 gcgccgagga tgaggttcct gcgtggctgg tgttcggcgt catgctgcgc gccgcggata
     7081 tggcccatgt cgagcaggtg cagaccgcca ctggggtttc gctgttgagc gaaggttttg
     7141 agatggtcga caccgacgca atcatcggca gcttcgcccg ccatctgatg accgccttcg
     7201 acctgtggaa cgagcgcggc tttgagacga tcgcacgcga ttatctggcg cggcttccca
     7261 gccgaaaggc cgccgagcgc cgaaggatcg accttagcgg cgaccttctg atcaccgcgc
     7321 caaccggcag cgggccaccc gagcgggcct ctcttgtgga tgcgctcgtc aaggcggcct
     7381 ggtatgatcc gcaagcgcgc ggtccgaaac tcggatgagg cggatatgtt gagatttccg
     7441 cggaccatca ggctcgaccc gtcggataca tttgttttcg agcgggcggc ggaaccaggc
     7501 gaatgggccg tttccggcgc gtttgtgttc tggaaccagg acccggcaaa cctcggccag
     7561 aaacagcgcg tggcgctgcg ttcgggcttt cttggcatcg acagcctcgg ctggtccacg
     7621 ctcgcgatcg tcaccgaggc gacggaggcg gacaggcaag ccatggtcga gcgcctcgca
     7681 agccagttgc aaaagaaatt cggcgcgcct gatctcaatg ccgcgcgtct tgccgccgaa
     7741 gaagaggtcg cgtttgcggc ctcgctatgc gatcatccgc cccagaccct gctcgccgtg
     7801 cagcgcagcg tcgagaacgg cgaaatccgt gagcgctttc gcacactcaa gccgcgcgaa
     7861 gccgcagcgg gcgccgaccg gttgcacgcg catgcccctg cctttacctt tcatgaggtc
     7921 gagggcgacg tcgagcctac cgaagaggtc gatcttcctg gaatgatcag gaacgaccgc
     7981 aggacggatc gcacatgagg gaattctggg tcgcctcggg tcatcatctg acccaccgcg
     8041 ccgagcacgg cgggctggtg gcgacgcccg aactgatcac ggcctatctg gcgcgccctg
     8101 aattgatgcc gccggccgac gcctgcgatg ccgagcgcaa cttgcatgca agcctgctgg
     8161 cggagcctct ccgcccggtt tcaagagcgg acattgccgc actcgccgac gccgatgcgc
     8221 gcgagaactg gacgttcatg gtgaacttcc gcgaccgcct gatatcggcg ccgtcgcttg
     8281 aggcgttgta tacgacactg gcgcgcaagg gctccggcga gctgccgccg atctttctat
     8341 cccagctctg ccatctgatc ttacgcaacg cgcttgaagg ttgcgacgat ccttacacgc
     8401 tgcgggccgg cgagctgttc tatcgcagcc agctggccgc catccacgat ggcgcgctgc
     8461 tgttggccga tgcggaagtc atcgaggcgc agcaacacac ccaacacgac ttgcattcgt
     8521 cgccgctgac agccttgctg cagccgaagc cgttcgccga gatggacgtg atggacgacg
     8581 agaatgcctg gacctactgg tcgcgctcgg acgcccacgc gatggtcatg aacatcggcg
     8641 gcaatgcgaa ggcgcgcgcc gggctgtgca gggcgatcga gcgatggatt agccacctgc
     8701 tcggcatcgc cgtccaagtg gaaacggtcg cttcgatcga ggaccgcgac tggcgctggt
     8761 tcgtcggcct cgacagcgag gcgacgcgga tcggcaatgc cttgtggaac ggcgccgcgc
     8821 tggatggcaa tgcctcggaa cgcatcgttg ccctgatgcg cctggacatc gaggacgcgc
     8881 ggcttgtgga tgaacgcgtc ggcaacaagc cgatctatct cattctggcg atgggcgccg
     8941 acagaacggt gcgcctgaaa ccgcagaatc tgatcgccgg gctgccgatc aagcatctgg
     9001 aggctgcaag ttgagccccg ccgccgtccc tttgctgcgt attcccgtag gcgttgtggt
     9061 tgaacgccgc aagacggact cgccgtggat cgatttcatc tggcgcggcg tcggcgtcct
     9121 gccggacgag cctgagatga agccgtggac ggttctacgc gagcaggatg aggcgacgct
     9181 gtactacgcc ggtagcgcta cggtcgatct ctaccgctcc gaaacccaac tctatcgcga
     9241 caatattgct tccggcaccc ccagcatctg gatcgtgctc agcccctcgg agggcagttg
     9301 gccctacgcc atttcggccg tcaccgccga tcccgcggaa ggcgaggctt tcacggaggc
     9361 agccgccaac ctggtcgaag cggtcgcgat gcctgaaatg cttcgcgacg cgattgaaaa
     9421 tttcatagcc gagcaccacg tcgaaaggga attcgtcaaa cgcgagcgac gccgcgccga
     9481 tccggaggcg ctggcgcggc ggcaacatga aggcgggcgc aaatgagcga cgatgaattc
     9541 cttgcgcgtt ggtctcgtcg caagcaagag gcgaggtccg gcaaccctgc tcccgaaccg
     9601 gctgagccga tgcaggcgca cgagccgacg ccaccttccg gcactgccga aatcccgccg
     9661 aacccggatc cagatctgtc aagcttgccg ccgatcgagt cgattgacgc ggcgaccgac
     9721 atcacggcgt ttcttcgcaa aggcattccg caggagctga gccgtgcggc gcttcgtcgc
     9781 gcctggactg ccgatcctgc catccgagac tttgtcgggt tggcggagaa cgcctgggac
     9841 ttcaacgatc cgaacgcgat gcccggcttc gggccgcttg attgctcggc ggagcagctc
     9901 aaggcgctcg ctgaccgggt tgtcggaggt gtgcggcagg taacagagaa gctcgccgac
     9961 tcgctcgaag aagccgaaaa tatgcggcga ttggctgctc cagagcacga tcccgcgctg
    10021 gacgaacaaa aatcgaatcc agtgggcacg attgccggct tgcctgcgac cgaccagtcc
    10081 atgagccccg acgtgtctcc ggctcctgct gcaccgcaac cggaaaccgc tgagagagcc
    10141 gagagcgggg aaactcctgt tcggcaccgc acccatggcg gtgcgctgcc ccgctagcgg
    10201 ttcgacgaaa ggctatagcc gcagactatg cctatcaatt gaccgctccc gaacccccgc
    10261 ctaatcttcg ctgaacaaag aataatcaag cacttggcgg cggggatggg aggtccaacg
    10321 tgcgcgaccc cgtactcaat cgcgcgatcg acgcggcagg cggcgtggtc cagcttgccc
    10381 gcaaaatcgg cattgcgcag ccttccattt cgaactggaa ccgcgttcct gcagagcggg
    10441 tgatcgccgt cgaagcggcg accggcgtgt cgcgtcaaca acttcgcccc gatctttata
    10501 gcgagcctgc cgtgaaagat gatgccgtcg atcccgtcga tgcggcgcgc gcccaggaat
    10561 acgcgttgct tgccgcattg ctttcgagcc cgccgtcgaa cgaactgctt ggtgagatcg
    10621 cgcggttgcg cggcgacgcc acgccgcttg gccgcgccca tgccgcgctg gccgaagctg
    10681 catcggaagc cacagccgag gtcgaccgcg aatatttcga cctgttcgtc ggcctcggac
    10741 gcggcgagct gttgccctac gggtcctact atctgaccgg ttttctcaat gagcgtccgc
    10801 tgtcgcgcct gcgcgatgat ctcgccgcac tcggcatcga gcgggtcgcg aataatttcg
    10861 aaccggagga tcatgcgggg acgctgtgcg agatcatggc gggcctcgcc gtcgggcgct
    10921 tcccggcatc ggcagaaatg cagcgccgat tcttcgagaa gcatgtggcg ccgtggatgg
    10981 gccgtctgtt tgccgacatg gagactgcca cgagcgccaa attctacaga tcggtcggat
    11041 cgcttggccg cctgtttctg aagatcgaaa ccgaagcatt tacgttcgcc aactgaccaa
    11101 cggccgttgg tccgggagag atgcgatgag ggacgaaaag aaaaccaagg ttggtcgccg
    11161 cgatttcctt cgcaaggtcg gcgtcggcgc ggccggtgcc ggcgcgacgc ttgcgacacc
    11221 gttgatggga actgcgcagg ccgacagcga gaacgacgat gagaaacgca agccgcgcta
    11281 caaggaaacc gatcacgtga aggcgtacta ccgcgtcaat cgttatcctg cttgaggagg
    11341 cggccgtgct gatcaagaga acacaaagcc agcgtttcag ctccgaccgt cgcggatccg
    11401 tcgcgggggc tctatcgggg cataccggcg gtctcgaccg ccgcgcattc ctgcgccggg
    11461 ccggccttgc cggcggcgcg ctcgccgcgc tgggcacgct gccgctcggc agcgtccgca
    11521 aggcggaagc agcggtggca ggcccgctct ctgcgggcgc agccgtccgc aaaagcatct
    11581 gcacgcattg cgcggtcgga tgcacggtga ccgcggaggt gctgaacgga gtatgggtcg
    11641 gccaggagcc gagctgggat tcgccgatca atcgcggctc gcattgcgcg aagggcgcat
    11701 cggtgcgcga actcgtgcac agcgttcggc ggctgcgcta tccgatgaag ctcgtcaacg
    11761 ggcagtggac gcgcctctcc tgggatactg caatcaacga gatcggcgac aagctgctcc
    11821 aaattcgcga gaagtcgggc ggcgactccg tctactggct cggatcggcc aagatgacca
    11881 acgaaggcgc ctatctgttc cgcaaatttg gtgccttctg gggaaccaac aacaccgacc
    11941 atcaggcgcg catctgccat tcaacgaccg tcaccggcgt ggccaatacc tggggctatg
    12001 gcgcgatgac caacagcttc aacgacatcc gcaatgccaa gacccagatc atcatgggcg
    12061 gaaatccggc c
//
LOCUS       DATNOI010000574        10897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1118683,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000574 DATNOI010000000
VERSION     DATNOI010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10897
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118683"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="VN968_23195"
     CDS             complement(<1..1333)
                     /locus_tag="VN968_23195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXL32173.1"
                     /translation="MKPGSARANDAIETKELARLSAVELLNGYGAGLFTPRDVIEDVI
                     AALERIDASCNVVVTSTYDQARAAADEATRAWASGRPQGLLAGVPVSIKDLVYVAGVP
                     AFGGAPVNKDLVPTVDAAAVTALKSAGAIITCKTTTCESGYKLTADSPVSGVTRNPWN
                     RERTSGGSSGGAAAAVAAGCGPLAIGTDGVGSIRVPSSFCGVVGVKPTFGLVPRSPGF
                     SPPSWASLAHTGPIARTVADAALMLEVIAIHDVRDAASLKVAPRAFDTHPGELDGLRI
                     GASVDFGYAAVDPDVRGAFQSALASLASLGAEILPDCVRFAPDMLDRILKPIAYTEQA
                     AAVADRGADALALSDGDYREIIAQGRSYVGVDYVEAGYRRNSLRASFLELFQTVDALV
                     TPTVAITAFPAGTLGVDRVAGVAVDRHLGWSPFSWPINLTGLPAATIPCGFD"
     gene            complement(1342..2145)
                     /locus_tag="VN968_23200"
     CDS             complement(1342..2145)
                     /locus_tag="VN968_23200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXL32174.1"
                     /translation="MISFAFHSPQTLPEAAALLGSVSDSMPFAGGMTLLPTIKQRLAS
                     PAALVDLSKIPQLSGIAIEGSTLVIGAMARHVDVAESARVRNSIPLLAALAADIGDPA
                     VRNRGTIGGSIANNDPSADYPAAVLALAATIVTDRRAIAADSFFTGLFETALEPGEVI
                     TAVRFPIPSAGGYAKFKSPASRYAVVGVCVVKAADQVRVAVTGAGPGVFRLAEMEAAL
                     AASFEAGAVASIKVSAANLTSDIHADPEYRAHLITVMAKKAVTAAIATL"
     gene            complement(2156..4543)
                     /locus_tag="VN968_23205"
     CDS             complement(2156..4543)
                     /locus_tag="VN968_23205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXL32175.1"
                     /translation="MSNIIGIGEAPRRKEDFRFLTGRGNYVADMKRPGSTFGVFIRSQ
                     HAHAVIRGIDKSAALALPGVEAVLTGDDVAADGLGSLPCGWGIHGADGLPMKEPPFPM
                     LAQGKVRFVGDMVAFVVADTLAQARAAAERVVIDYEVLPAVVGVLEAVRPGAPQLFDD
                     VPNNLCCDWELGDKAAVAVAFRKAAHVARLSLVNNRLIGNPMEPRAAIAEYNAGTGHY
                     TLWTTSQFPHVVRFLMAALVLNIPEQKMRVVAPDVGGGFGVKQFHYGEEAVITWAAKR
                     VGRPIKWVADRSEGFVSDRHGRDHLTEAELALDENGKFLALKVATLANMGAYLSTFGP
                     NIPTNLYAPLLGGVYTTPAIYCNVKVVFTNTVPVDAYRGAGRPEATFVVERIVDVAAA
                     EMGIDRVELRRRNIIPKEAYPYQTPVLVEYDSGDPMGCLEGALVAADVAGFAGRKAAS
                     ERDGKFRGLGYSTYIEACGLAPSRFAGRLGARGGLYESATVRVHPTGQVTVLIGTHNH
                     GQGHETTFAQIVSEKLGVSFDNVDIVFGDTDRVQFGMGTYGSRSLVVGGAALSKASDK
                     VVMKGKKIAAHLLEAGEHDIQFEAGVFSVAGTDRSKTFQEIAGAAYVPHNYPLEVLEP
                     GLEEQAYYDPVNFTYPGGCHIAEVEIDPDTGIVTLERYTAVDDVGTVINPMIVEGQLH
                     GGIVQGVGQALYESAVYDEVSGQLLSGSFMDYTMPRADHVPNMNVQTHSTRCTHTPMG
                     VKGCGEVGTIGSPAAVINAVVDALSHLGVQHVDMPATPNRIWRVIQNAAMPRAAE"
     gene            complement(4547..5035)
                     /locus_tag="VN968_23210"
     CDS             complement(4547..5035)
                     /locus_tag="VN968_23210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL32176.1"
                     /translation="MIVNFTLNGRSRTVDVPPATLVSELLRETLNLTGTHVGCDTSQC
                     GACVVLFNGEAVKSCTLLAPILDGGTLMSIEGLAGAGGSNQLHPMQEAFHQNHGLQCG
                     FCTPGMVMTAVAMAAKNPALTEAEVRRGLEGNICRCTGYQNIVASVLAGAAAMNASAI
                     GD"
     gene            complement(5060..6166)
                     /locus_tag="VN968_23215"
     CDS             complement(5060..6166)
                     /locus_tag="VN968_23215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32177.1"
                     /translation="MRPGLGDIELAGLCKSYDGVTNVVDGVNLKIPDGAYCCFLGPSG
                     CGKTTILRMIAGHEDPSAGEIVIGGDNVVGLAPVERRTAMMFQSYALFPHLTVRDNIA
                     FALRVRGQSRAERYRAADMMMEKVRLTELADRLPANLSGGQQQRVALARAAITEPRVL
                     LLDEPLSALDEQLRVQMRQELRRMQRELGITFVHVTHTQLEAIALADIVVVMEKGRIK
                     QSGAARDVYAHPRDRYVAEFLGGQNVLSGKVEKVSDDHIVISQPNRSGIQVGVRPSAS
                     VGIGDRIDLAVRRDDVELMRATSGGPGNGATALPSRVVAVEYQGSFVKVMLDTIPDEE
                     FIAYVPERIFFRDPFNVGDVLLATWSAGRARILA"
     gene            complement(6178..7038)
                     /locus_tag="VN968_23220"
     CDS             complement(6178..7038)
                     /locus_tag="VN968_23220"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL32178.1"
                     /translation="MAKPRRNVVARRGDSRPWTFYALATVFTLYVAALYGPMICIYIL
                     SFQDIRGGLVFPMKGHSLHWFVDLFTQVRTGDVKGSFDRSIKLAVLVTVITLVFSFMA
                     GMAFRRRFVGDTFVFYLMIGSLVAPGLVLGLGIGLLFQWVGITASWYTSALGAQLSWT
                     LPFGVLVMFAVMSRFDNVWEEAARDLGASRWQIVRLVVLPVLAPGLVAVALFGFTLSY
                     DEFARTLQTAGSLNTLPLEIWSMTLNVTSPSLYALGTVTTVVSFVIIGVSIGAIILIN
                     KRRGVRAGSH"
     gene            complement(7068..7928)
                     /locus_tag="VN968_23225"
     CDS             complement(7068..7928)
                     /locus_tag="VN968_23225"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL32179.1"
                     /translation="MLRHNLSSWLYVSPLMLVLVPFFLMPIVVVLAASFLETDGFGGL
                     LPSFTLANYLDVLTSAQTFHLYMATLKFTVLTWFFTLIIGFLVAYFLVFHVRNQLLAI
                     GLFLVCTVPFWTSNIIRMISWIPLLGKEGLVNSALLGTGLIREPLEVLLYSGLAVVIA
                     YVHQLTIFMVVPIFNSMARIDKRIVEAALDAGASRLDVMRFIVVPMSKSGIALGSIFV
                     ISIVMGDFFVVKVMSGGSSASVVSAFYENVGVLQYPIAAASAVILTLVLIAVISLILR
                     AVDIRREITQ"
     gene            complement(8026..9372)
                     /locus_tag="VN968_23230"
     CDS             complement(8026..9372)
                     /locus_tag="VN968_23230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXL32180.1"
                     /translation="MLDDDNAGNSASSLKTPTKTKQLSRRAVLKAGAAATGAAIGSDV
                     VRGFPTIWAQEIKDIELRHVGVSYSVVKAIGDQAAKDLGFKVTMQNLDTSAAINRFIS
                     QPDSVDIADLEGWQAKLAAKRSVIQGIEVKKIKEFDNILPIFTKGEIDGHKIPRQGIS
                     PYEAMYLEKPNSTELHDGVTEWATFLPQVYNADSIGYRPDLVGHEVTEWKDLIDPKFK
                     GKAAILDVPAIGIMDAALCFESADLIKYGNKGNMTKEEIDFTCNKLIELKKSGQFRAT
                     WTTFDQSVQLMAAGEVVIQSMWSPAVAAVRVKEIPCVYAPVNVKNGKEGYRGWCNGMG
                     LMKHLSGKKLDAAYEYLNWYLSGWQGGFVGRYGYYSPVPSTAKKFLTAAEWDFWYDGK
                     PAPSVINDPYGVPMEKAGTKRDGGSFVERVKNISCWNTLMDEAAYMNKRWNDFKVA"
     gene            9662..>10897
                     /locus_tag="VN968_23235"
     CDS             9662..>10897
                     /locus_tag="VN968_23235"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXL32181.1"
                     /translation="MRKAEGYKRVVTSRSRAIITVIILTLLVTPTVLRAQTIPGAQAE
                     SISALSRGEWPAYAGTYASARYSPLTQIDRTNAKDLHVAWRWKSPDQAVKDANPKVGP
                     TRANESTPLMVGGTLYTSTSLSQVAAIDAASGATKWVFDPKIYENGLGIPANDGWLHR
                     GVGYWRNGDDERIVMLTAFAQMIALDARTGKPIPTFGTDGRVDLALGMRRPVDRDYYT
                     MSSPPVIVRGVIVVGSSVMDWWGHRPSPPGDVRGFDVVTGRLLWTFHTVAQAGEPGVE
                     TWEKDSWKEAGNANVWAPMSADEELGYVYLPVSTPTNDYYGGHRPGDGLYGESLVCLD
                     AATGKKVWHYQLVHHGLWDYDTPAAPNLIDITVAGRPIKAVAQVTKQAFVYVFDRVTG
                     QPVWPIEEQSVPASSVPGEH"
BASE COUNT         2069 a         3493 c         3412 g         1923 t
ORIGIN      
        1 tgtcgaaccc gcagggaatc gtcgcagccg gcaatccggt caggtttatc ggccacgaga
       61 acggtgacca gccgaggtgg cgatcgacgg cgacgccggc aacgcgatcg acacccagcg
      121 ttcccgctgg gaacgctgtg atggcgacgg tcggggtcac tagcgcatcg accgtttgaa
      181 ataattccag gaacgacgcg cgcaggctgt tgcgccgata gccggcctcg acatagtcga
      241 caccgacata gctgcggcct tgcgcgatga tttcgcggta gtccccgtcg gacaaggcga
      301 gcgcatcggc gccgcgatcc gcgaccgccg ccgcctgctc ggtataggcg atcggcttca
      361 agatgcgatc cagcatgtcc ggagcgaatc tgacgcagtc aggcaagatc tcggcgccaa
      421 gtgacgccag cgaagccagc gctgactgga atgccccgcg cacgtcgggg tcgaccgcgg
      481 cgtagccgaa gtctacgctg gcgccgatcc tcagtccgtc cagttctcca ggatgggtgt
      541 cgaaggcacg cggcgccacc ttcaaactcg cggcatcgcg aacatcatga atggcgatca
      601 cctccagcat cagcgcggcg tccgcgacgg ttcgcgcgat cggcccggta tgcgccagcg
      661 acgcccacga cggcggcgaa aatccgggcg acctcggcac cagaccgaag gtcggcttca
      721 cgccaacgac gccgcagaac gaggacggca cgcgaatcga accgacgcca tcagttccga
      781 tcgcgagcgg gccgcatccg gcggcaaccg cagccgcagc gcctccgctc gacccgccgc
      841 tggtccgctc cctgttccag ggattgcggg tgacgccgga gacagggctg tcggccgtga
      901 gcttgtatcc ggactcgcac gttgtggtct tgcaggtgat gatcgcgccg gccgatttca
      961 acgccgtgac cgcggcggca tcgacggtcg gcaccaggtc cttgttcaca ggcgcgccgc
     1021 cgaacgccgg cacgcccgcg acgtagacga gatccttgat gctgaccggc acgcctgcca
     1081 gcagcccctg tggcctgccg gatgcccatg cgcgtgtcgc ttcgtcggcg gcggcgcgcg
     1141 cctggtcgta ggtcgacgtg acgacgacat tgcaggatgc gtcgatccgt tcgagcgccg
     1201 cgatgacgtc ctcgatgaca tctctcggcg tgaaaagccc ggccccatag ccgttgagca
     1261 gctcgacggc ggagagtcgt gccagttctt tggtttcaat cgcatcgttg gcgcgagcgc
     1321 ttcctggctt caccgcatgc ctcacaaggt ggcgatggcg gccgtcacgg ccttcttcgc
     1381 catgacggtg atgagatggg cgcgatattc ggggtcggcg tgaatgtcgg aagtcaaatt
     1441 tgccgcgctg accttgatcg aggccaccgc gccggcctca aacgaggctg cgagcgcggc
     1501 ctccatttcg gccagccgga aaacgccggg gcccgctccg gtcaccgcga cgcggacctg
     1561 gtcggcggcc ttgacgacgc agacgccgac caccgcgtag cgcgaggcgg gagacttgaa
     1621 tttcgcatag ccgccggcgg atggaatggg aaagcgaacg gccgtgataa cctcgcctgg
     1681 ttccagcgcg gtttcgaaca gtcccgtgaa aaaactgtcg gcggctattg cacggcggtc
     1741 ggttacgatc gtggccgcca gcgccagcac cgcggccgga taatcggcgg agggatcgtt
     1801 gttggcaatc gagccaccga tggtgcctcg gttgcgcacc gcgggatcgc cgatatcagc
     1861 ggcgagcgca gccagcaacg gaatgctgtt gcgcacacgt gcggattccg cgacatcgac
     1921 gtggcgtgcc atcgccccga tcaccaatgt actaccttcg atggcaattc cggacagttg
     1981 cggaattttc gacagatcga ccagcgcggc cggcgacgcc agccgctgct tgatcgtcgg
     2041 aagcagcgtc atgccgccgg cgaacggcat gctgtcggaa acgctgccga gcagtgccgc
     2101 cgcctccggc agtgtctgcg ggctgtgaaa cgcgaaagat atcatctgac tgctcctact
     2161 ctgcggcgcg cggcatcgcc gcattctgga tgacgcgcca gatccggttg ggggtagcgg
     2221 gcatatcgac atgctggacg ccaaggtgcg aaagcgcatc gaccaccgca ttgatcaccg
     2281 ccgcgggcga tccgatggtc ccgacctcgc cgcatccctt gacgcccatc ggcgtgtgcg
     2341 tgcagcgcgt cgaatgggtt tgcacgttca tgttcggaac atggtcggcg cgcggcatgg
     2401 tgtaatccat gaacgagccg gacagcagct gcccggagac ctcgtcataa accgcgcttt
     2461 cgtacagtgc ctgaccgacg ccctgaacga tcccgccatg caactgccct tcgacgatca
     2521 tcggattgat gacggtgccg acgtcatcga cggcggtgta tcgctcgagc gtgacgatcc
     2581 cggtgtcggg atcgatttcc acctcggcga tatgacagcc gccgggatag gtgaagttga
     2641 ccggatcgta gtaagcctgc tcctcgaggc cgggctccag cacttccagc ggataattgt
     2701 gcgggacgta ggcggcgccg gcgatctcct ggaaggtctt gctgcggtcg gtgccggcaa
     2761 ccgagaaaac cccggcctcg aactgaatat cgtgttcgcc ggcctcgagc aggtgcgccg
     2821 caatcttctt gcctttcatc accaccttgt cgctggcctt ggacagcgcg gcgccgccca
     2881 ccaccagcga gcgcgagccg taggtgccca tgccgaactg cacccgatcg gtatcgccaa
     2941 acacgatatc gacattgtcg aacgacaccc cgagtttctc cgacacgatc tgcgcgaagg
     3001 tcgtttcatg gccctggcca tgattgtggg tgccgatcag gaccgtcact tgtccggtcg
     3061 gatgcacccg cacggtggcg ctctcgtaaa gtccaccgcg ggcgccgagg cgcccggcaa
     3121 agcgcgacgg cgccagcccg caggcctcga tataggtcga atagccaagt ccacggaact
     3181 tgccgtcgcg ttccgacgcc gccttgcgcc cggcaaagcc ggcgacatcg gccgcaacca
     3241 gcgcgccctc caggcatccc atcgggtcgc cggagtcgta ttcgaccagc accggggtct
     3301 ggtagggata ggcttccttg ggaatgatgt tgcgccggcg caattcgacg cggtcgatgc
     3361 ccatctcggc cgctgcgacg tcgacgatgc gttcgaccac gaaggtcgct tccggccgcc
     3421 ccgcgccgcg ataggcgtcg accgggacgg tgttggtgaa gaccaccttg acgttgcagt
     3481 agatcgccgg cgtcgtgtag acgccgccga gcagcggggc atagagattg gtcgggatat
     3541 tgggcccaaa ggtcgagagg taggcgccca tattggccaa tgtggcgacc ttcaaagcga
     3601 ggaattttcc gttctcgtcg agcgccagtt cggcttcggt caggtgatcc cggccatggc
     3661 gatcggacac aaagccctcc gaacggtccg cgacccactt gatcggccgc ccgacccgtt
     3721 tggccgccca ggtaatgacc gcctcttcgc catagtggaa ttgcttgacg ccgaagccgc
     3781 cgccgacatc cggcgcgacc acgcgcatct tctgttcggg tatgttgaga accagggcgg
     3841 ccatcagaaa ccgcaccaca tgcgggaact ggcttgtcgt ccacagcgtg tagtgcccgg
     3901 tgcccgcgtt gtattctgcg atcgccgcgc gcggctccat cggattgccg atcagtcgat
     3961 tgttgacgag acttagccgg gcgacatggg ccgccttgcg gaaggcgacc gcgacggcgg
     4021 ccttgtcgcc gagctcccag tcgcagcaga ggttgttcgg cacgtcgtcg aacagctggg
     4081 gagcgcctgg acggacggct tcgagcaccc cgaccacggc cggcaagact tcgtaatcga
     4141 tgacgacacg ttcggcggca gcgcgggcct gcgccagcgt gtcggcaacg acgaatgcca
     4201 ccatgtcccc gacaaagcgc accttgccct gtgccagcat cgggaacggc ggctccttca
     4261 tcggcagccc atcggccccg tgaatgcccc agccgcaggg cagcgatccg agaccgtcgg
     4321 ccgccacgtc gtcgccggtc agcacggcct cgacccccgg aagggcaagg gctgcggatt
     4381 tgtcgatgcc cctgatcacc gcatgggcat gctgggaacg gatgaatacg ccgaacgtcg
     4441 aacccgggcg cttcatgtcg gccacatagt tgccgcgacc ggtcagaaag cgaaagtctt
     4501 ccttgcggcg cggtgcttcg ccaattccga tgatgttgga catgggtcag tctccgatcg
     4561 cggatgcgtt catggccgcc gcaccggcca gaaccgatgc cacgatgttc tgatagccgg
     4621 tgcaccggca gatattgcct tccaggccgc gacgaacctc ggcttcggtc agcgccgggt
     4681 ttttcgcagc catcgccacc gcggtcatca ccatgccggg cgtacagaac ccgcattgca
     4741 ggccgtgatt ctggtgaaac gcttcctgca tcgggtgcaa ctgattggag ccgccggcgc
     4801 cggccaatcc ctcgatcgac atcagcgtgc cgccgtcgag aatgggcgcg agcagggtgc
     4861 agctctttac ggcctcgccg ttgaacagca cgacgcaggc gccgcactgg ctggtgtcgc
     4921 agccgacgtg agtgccggtg agattcagtg tctcgcgaag cagctccgaa acaagcgtcg
     4981 ccggcgggac gtccaccgtt cgggatcggc cgttgagggt gaaattaacg atcatgggac
     5041 ctccgttact gggctggcat caggcaagga tgcgggcgcg gcccgccgac caggtggcga
     5101 gcaggacatc gccgacattg aacggatccc ggaagaaaat tcgctccggg acgtaggcga
     5161 tgaattcctc gtcgggaata gtgtcgagca tcaccttgac gaacgacccc tgatattcga
     5221 ccgcgaccac ccggctgggc agcgccgttg ccccgttgcc ggggcccccc gaagttgccc
     5281 gcatcagttc gacgtcgtcg cggcggaccg ccagatcgat gcgatcgccg atcccgacgc
     5341 tcgcgctcgg ccgcacgccg acctgaattc cgcttcggtt cggctgcgag atgacaatgt
     5401 ggtcgtcgct gaccttctcg accttgccgg acagtacatt ttggccgccg agaaattccg
     5461 cgacatagcg atcgcgcggg tgggcataga cgtcgcgcgc cgcgccggac tgcttgatcc
     5521 tgcccttttc catcacgacg acgatatcgg cgagcgcgat cgcctcgagc tgggtgtggg
     5581 taacatgcac gaaggtgatg ccgagttcgc gctgcatgcg ccgcagttct tgccgcatct
     5641 gcacccgaag ctgttcgtcc agcgcggaca gcggttcatc cagcagcaag acgcggggtt
     5701 cggtgatggc cgcgcgcgcc agcgccacgc gctgctgctg gccgccggac agattggccg
     5761 gcagacgatc cgcaagctcc gtcagccgca ccttttccat catcatgtcg gccgcccggt
     5821 agcgttcggc cctggattgg ccacggaccc gcagcgcgaa ggcgatatta tcgcggacgg
     5881 tgaggtgcgg aaacaacgcg taggactgga acatcatcgc cgtccggcgc tcgaccggcg
     5941 ccagccccac gacattgtcg ccgccgatga cgatttcgcc ggccgatggg tcctcgtgcc
     6001 ccgcgatcat acgcaggatc gtcgtcttgc cgcagccgga cggccccagg aaacagcaat
     6061 aggcgccgtc ggggatcttc aggttgacgc cgtcgaccac attggtcacg ccgtcatagc
     6121 tcttgcagag ccccgccagt tcgatgtcgc cgagacccgg gcgcatggcc gaattcctca
     6181 atggctgccg gcgcggacgc cgcgacgctt gttgatcagg atgatggccc cgatgctgac
     6241 gccaataatg acgaatgaaa ccaccgtggt caccgtgccc agcgcataga gcgagggcga
     6301 cgtcacgttg agcgtcatgc tccagatctc cagcggcagg gtgttgagcg agccggccgt
     6361 ctgcagggtg cgggcgaact catcgtaaga cagcgtgaac ccgaacagcg cgaccgcgac
     6421 cagcccgggc gccagcaccg ggagcaccac cagccggacg atctgccagc ggctggcccc
     6481 gaggtcgcgc gccgcttctt cccatacgtt gtcgaagcgc gacatcaccg cgaacatcac
     6541 cagcacgccg aacggaagcg tccacgacag ctgcgcgcct agcgccgagg tgtaccagct
     6601 cgcagtgatg ccgacccact ggaacaacag gccgatgccg agcccgagca ccagcccggg
     6661 cgccaccagg ctgccgatca tcagatagaa cacgaaggtg tcgccgacga accggcggcg
     6721 aaacgccatg ccggccatga acgagaacac cagcgtgatc acggtgacga gaacggcgag
     6781 cttgatcgac cgatcgaacg agcccttgac gtcgccggtg cgcacctggg tgaaaagatc
     6841 gacgaaccaa tgcaacgagt ggcccttcat cggaaacacc aggccgccgc gaatatcctg
     6901 gaacgacagg atgtagatgc agatcatcgg gccatacagt gctgcgacgt agagcgtgaa
     6961 aacggtggcc agcgcataga acgtccaggg ccggctgtcg ccgcggcgcg ccaccacgtt
     7021 gcgccggggt ttggccatcg cgatatcaga actttccatt gcgaccgtca ttgggtgatc
     7081 tcgcggcgta tatcgacggc gcgaaggatc agcgaaatca ctgcgatcag caccaaagtg
     7141 agaattaccg cacttgccgc ggcgatcgga tattgcagca cgccgacgtt ttcatagaac
     7201 gcgctgacca cggaagccga actgccgccg gacatcacct tgaccacgaa gaaatcaccc
     7261 atcacgatcg agatcacgaa aatcgatccc agcgcgatgc cgctcttcga catcggaacc
     7321 acgatgaaac gcatgacgtc gagccggctc gcaccggcgt cgagtgcggc ttcgacaatg
     7381 cgcttgtcga tgcgcgccat cgaattgaag atcggaacca ccatgaagat cgtcagttga
     7441 tggacatagg cgatcaccac ggcgaggccc gaatacagca gcacctccag cggctcacgg
     7501 atcaagccgg tgccaagcag cgctgaattc accagccctt cctttccgag tagcggaatc
     7561 caggagatca tgcggatgat gttcgaggtc cagaacggca ccgtgcagac caggaacagc
     7621 ccgatcgcaa gcagctggtt tcggacatgg aacaccagga aataggcgac cagaaatccg
     7681 atgatcaggg tgaagaacca ggtcagcacc gtgaatttca gcgttgccat atagagatga
     7741 aaagtctgcg ccgacgtcag cacgtcgaga tagttcgcca gcgtaaagct cggcagcagg
     7801 ccgccaaagc cgtcggtctc gaggaagctc gcggcgagca ccacgacaat cggcatcagg
     7861 aaaaatggaa ccagcacgag catcagcggc gagacgtaca gccatgaact cagattgtgc
     7921 cgcagcatcg gttaaggcct gcctcgattt tgtccggggt caaccggttc tgaactgtgc
     7981 ggcgatgtcc gccggtgcgc acgccgcacc ggcagaagtt ttcgatcagg cgaccttgaa
     8041 gtcgttccag cgcttgttca tgtaggcggc ctcgtccatc agcgtattcc agcaggagat
     8101 gttcttgacg cgttcgacga aggagccgcc atcccgcttg gtgccggcct tttccatcgg
     8161 cacgccgtag gggtcgttga tgaccgaggg cgccggcttg ccgtcgtacc aaaaatccca
     8221 ttccgccgcg gtgaggaact tcttggcggt cgacggcacc ggactgtagt agccgtagcg
     8281 tccgacgaag ccgccctgcc agcccgacag ataccagttg agatactcat aggcggcgtc
     8341 gagcttcttg ccggacaggt gcttcatcaa gcccatgccg ttgcaccagc cgcgatatcc
     8401 ttccttgccg ttcttgacgt tgacgggtgc atagacgcaa gggatctcct tgacccgaac
     8461 cgcggcgacg gccggcgacc acatcgactg gatcacgact tcgccggcgg ccatcaactg
     8521 gaccgactgg tcgaaggtgg tccaggtcgc gcggaactgg ccggacttct tcagctcgat
     8581 cagcttgttg caggtgaagt cgatctcttc cttggtcatg ttgcctttgt tgccgtactt
     8641 gatcagatcc gcgctttcga agcataaagc ggcgtccatg ataccgatcg ccggaacgtc
     8701 gaggatcgcc gccttgccct tgaatttcgg atcgatcagg tctttccact cggtgacctc
     8761 gtggccgacc agatccgggc gatagccgat cgagtccgcg ttgtagacct gcggcaggaa
     8821 cgtcgcccat tccgtgacgc cgtcgtgcag ttcggtcgag ttcggcttct cgagatacat
     8881 cgcctcatag ggtgaaatgc cctgacgcgg aatcttgtga ccgtcgatct cgcccttggt
     8941 gaagatcggc aggatattgt cgaattcctt gatcttcttg acttcaattc cctgaatcac
     9001 cgagcgcttc gcggccaatt tggcctgcca gccttcgagg tcggcgatgt caaccgaatc
     9061 cggctggctg atgaaccggt tgatggccgc cgaggtatcg aggttctgca tggtgacctt
     9121 gaagccgagg tcctttgcgg cctgatcgcc gatcgccttg accaccgaat aggacacgcc
     9181 gacgtgtctt aattcgatat ccttgatctc ctgcgcccag atcgtcggaa agccgcgcac
     9241 gacatcggag ccgatcgcgg cgcccgtcgc cgcggcgccg gccttgagaa ccgctcgccg
     9301 tgaaagttgt ttggtcttcg tcggggtctt caacgaagat gcgctattac ctgcattgtc
     9361 atcatccaac ataactatct ccgttgcgtg ttgatggact aacgctcgcg gcaagacggc
     9421 tctttctcgc gaccgtgtcg gcggctttga ggtaacgaca gggcaatcgg cttgtaaaat
     9481 cggaagtgga aatgcgagcc ataagggctg ctgatggtgt ccgcgtttgc ctgccgcagc
     9541 ggcacgtccc acctaaaatt tgacctttac ttcccgaaat tcaaccgcgc ccgacctgtt
     9601 ctcctttgac tgtgctcccg ttctcggaga tgcttgccta aaaatcgggg agggtacggc
     9661 attgcgcaaa gcggaagggt acaagcgggt cgtcacgtcg cgcagccgcg cgataatcac
     9721 ggtcatcatt ctgacgctgc tggtcacccc gaccgtgctt cgtgcacaaa ccatccccgg
     9781 cgcgcaggcg gaatccatca gcgcgttatc gcgcggcgaa tggcccgctt atgccggcac
     9841 ctatgcctcg gcccgttatt cgccgctcac ccagatcgac cggaccaacg cgaaggacct
     9901 gcacgtcgcc tggcgctgga agtcgccgga tcaggcggtc aaggacgcca atccgaaggt
     9961 cggtcctacc agagccaatg aatcgacgcc gctgatggtc ggcggcacgc tctatacctc
    10021 cacgtcgctg tcgcaggtcg ccgccatcga tgccgcgtca ggcgcaacca aatgggtgtt
    10081 cgacccgaag atctacgaaa acggtctcgg tattcccgcc aacgacggct ggctgcatcg
    10141 cggcgtcggc tattggcgca acggcgacga cgaacgcatc gtgatgctca cggcgtttgc
    10201 gcagatgatc gcgctcgatg ccaggaccgg caaaccaatc ccgacattcg ggaccgatgg
    10261 ccgtgtcgat ctcgcactgg gcatgcggcg gccggtcgac cgcgattact acaccatgag
    10321 ttcgccgccg gtgatcgtgc gcggggtgat cgtggtcggt tcgtcggtga tggactggtg
    10381 gggacaccgg ccgtcgccgc ccggcgacgt gcgcggcttc gatgtcgtca ccggccgtct
    10441 gctctggacc ttccacaccg tggcgcaagc cggtgagccg ggggtcgaga cctgggagaa
    10501 ggattcctgg aaggaagccg gcaatgccaa tgtctgggcg ccgatgagcg ccgacgagga
    10561 actcggctat gtctacttgc cggtcagcac gccgaccaac gattattacg gcggccaccg
    10621 ccccggcgac ggtctctacg gcgagagtct cgtgtgtctc gacgcggcga ccggcaagaa
    10681 ggtctggcac tatcagctcg tccatcacgg cctgtgggat tacgacacgc cggccgcgcc
    10741 gaatttgatc gacatcaccg tcgctggaag acccatcaag gccgtcgcgc aggtgaccaa
    10801 gcaggccttt gtctatgtgt ttgatcgcgt caccggccag ccggtgtggc cgatcgaaga
    10861 gcagtcggtg ccggcctcca gcgtgcccgg cgagcac
//
LOCUS       DATNOI010000575         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1320084,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000575 DATNOI010000000
VERSION     DATNOI010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320084"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VN968_23240"
     CDS             <1..798
                     /locus_tag="VN968_23240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXL32182.1"
                     /translation="PRVVAFGEPVPKGGGTYRVGKPYTVAGRVYVPEEDTEYREDGLA
                     SWYGNDFHGRLTANGEVFDMASLTAAHPTLPMPCYARVTNLSNGKSLIVRVNDRGPYH
                     GNRLIDVSNKAAELLEFKGNGIARVRVEYVGRAPLEGSDDRQLVATLRTGVPAPSPSM
                     VRVASARPFVPEIPSSARAIRGEIPMPEGRPYSLGNTSADLASISATSEMSASRRTRS
                     GGRMIENPRAVSYETDDRYVPSVRPVSAYAPVDPRGPSELLAGRGLY"
     gene            complement(806..1759)
                     /locus_tag="VN968_23245"
     CDS             complement(806..1759)
                     /locus_tag="VN968_23245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL32183.1"
                     /translation="MSSSRTISANGVEIFLLEQGEGPLVVLCHGWPELSYSWRHQIAA
                     LAAAGFHVVAPDMRGFGRTSAPADISAYSIFDTVGDMVALVAALGENRAAIVGHDWGA
                     PVAWHAALFRPDIFSAVAGLSVPPPRRGRGRPLDTLRESGITNFYWQYFQTPGVAEAE
                     FERDVGLAMRTLLGRGFSDPASLFVEDGKGFLGNPNADRPLPHWFGEADLAYFAEAYR
                     KSGFRGGLNWYRNIDRNWELTAPWQGAQIHQPSLFIAGSKDSVITGLIGAKHVADMER
                     VLPNLKQKLIVDGASHWIQQERAAEVNAALIAFLNENALSE"
     gene            1923..>2323
                     /locus_tag="VN968_23250"
     CDS             1923..>2323
                     /locus_tag="VN968_23250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HXL32184.1"
                     /translation="MAPEISPFRKPKSATPAFWRSLVAAVVAIAVGWGGVVYAANNSV
                     QGAKKEEGGFDGDAPTAILIEATSGSVLFEKNADELRAPSSMMKLMTAELVFHAIKQG
                     EVKLTDEYRVSENAWRKGGAPSGGSTMFAAI"
BASE COUNT          441 a          732 c          731 g          419 t
ORIGIN      
        1 ccccgggttg ttgcttttgg cgaacccgtc ccgaagggcg gcggcaccta ccgcgtcggc
       61 aagccctata cggtggcggg ccgggtttat gtgccggaag aagacaccga ataccgcgag
      121 gacggtttgg cctcgtggta cggcaacgat ttccatggac ggctgaccgc caatggcgaa
      181 gtgttcgaca tggcttcgct gaccgccgcc catccgaccc ttccgatgcc ctgttatgcg
      241 cgcgtgacca atctctctaa cggcaaatcc ctgatcgtcc gcgtcaatga ccggggacca
      301 tatcacggca accggctcat cgatgtctcg aacaaagccg ccgaactgct tgaattcaaa
      361 ggcaatggca tcgcgcgggt gcgggtcgag tatgtcggcc gggcgccgct cgaaggttcc
      421 gacgaccgtc agctggtagc aaccttgcgt accggcgtgc cggcgccatc gccctccatg
      481 gtccgggtcg cgtcagcccg tccgttcgtt ccggaaattc cgtcatccgc ccgtgcgatc
      541 cggggggaaa ttccaatgcc ggaggggcgg ccctacagcc tcggcaatac gtcggcggac
      601 ctggcctcga tatcagcgac atcggaaatg tccgcctcca ggcgcacgcg ttccggcggc
      661 cgcatgatcg agaatccacg cgcggtctcc tatgagaccg atgaccgtta cgtgccgagc
      721 gtcaggccgg tttccgccta tgcgccggtc gatccgcgcg gtcccagcga gttgctggcc
      781 ggacgcgggc tctactgagc cgcgcttatt ccgacagcgc gttctcgttg agaaaagcga
      841 tcagagccgc gttgacctca gcggcgcgtt cctgctgaat ccagtggctg gccccatcga
      901 cgatcagctt ctgtttcagg ttcggcagaa cccgctccat gtcggcgacg tgtttggcac
      961 cgatcagacc ggtgatgacg gaatccttcg atcccgcaat gaacagtgac ggctggtgga
     1021 tttgcgcgcc ttgccatggc gcggtcagtt cccagttgcg gtcgatgttg cggtaccagt
     1081 tcagcccgcc gcggaagccg gactttcggt aggcctccgc gaaataagcg aggtcggctt
     1141 cgccgaacca atgcggcaac ggccggtcgg cgttcggatt gccgagaaag cccttgccat
     1201 cttcgacaaa cagagaggct ggatcggaaa atccgcgacc aagcagggtc cgcatcgcca
     1261 agccaacatc gcgctcgaac tcggcttcgg cgacgcctgg cgtctgaaag tactgccagt
     1321 agaaattggt gatgccgctt tcgcgcagcg tatcgagcgg ccggccacgg cctcgccgag
     1381 gcggcgggac gctgaggccg gccacggcgg agaatatatc cggacggaac agcgcggcgt
     1441 gccaggcgac cggcgcgccc cagtcatggc cgacgatggc cgccctattt tcgccaagcg
     1501 cggcgaccag cgcgaccatg tctccaaccg tatcgaagat gctgtacgca ctgatatcgg
     1561 caggcgcgct ggttcggccg aaaccccgca tatccggcgc caccacatgg aaacccgcgg
     1621 ctgcaagcgc cgcgatttga tgccgccacg aataggacag ttccggccag ccatggcaaa
     1681 gcaccaccag cggaccttcg ccctgttcga gcagaaagat ttcgaccccg ttggcggaaa
     1741 tggtgcgtga ggatgacatc gcttttccgc cttgtttggg agattttgtt taagagcggc
     1801 aatgcggcac gataggggtg tttgactggt gccgcaatcg gatcggctcg cgagacgtcc
     1861 gcaaaacccg tgttgtttgc gcaacttcca actgttagaa cgccgacttc aaggcagcgc
     1921 cgatggcacc cgaaatatca ccgttccgca aacctaagtc cgcgacacca gctttctggc
     1981 gcagtctggt tgcggccgtc gtcgccatcg cggtcggctg gggcggcgtg gtctacgccg
     2041 ccaataacag cgtgcagggc gccaagaagg aggagggggg ttttgacggc gatgcgccga
     2101 ccgcgatcct gatcgaagcc accagcggca gcgtgctgtt cgagaagaac gccgatgagc
     2161 tgagggcccc gtccagcatg atgaagctga tgaccgccga attggtcttc catgccatca
     2221 agcagggcga ggtcaaactc acggatgaat atcgggtcag cgagaatgcc tggcgcaagg
     2281 gcggcgcccc ctcgggcgga tcgaccatgt tcgccgccat cca
//
LOCUS       DATNOI010000576         7877 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_424798,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000576 DATNOI010000000
VERSION     DATNOI010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7877
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424798"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VN968_23255"
     CDS             <1..490
                     /locus_tag="VN968_23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HXL32185.1"
                     /translation="HGHALPERIDVGTMVEVPALLYQLDELLKKVDFISVGSNDLFQF
                     LFAVDRGNAKVSERFDTMSAPILRALREIVRKANAAKKSASLCGEMAAKPLGALALIA
                     LGYRSLSMSATGHGPVKAMILDLDAKKAEAMMAPLLDAPSGSVSIREKLTEFAEAEGL
                     SL"
     gene            545..1630
                     /gene="prfA"
                     /locus_tag="VN968_23260"
     CDS             545..1630
                     /gene="prfA"
                     /locus_tag="VN968_23260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HXL32186.1"
                     /translation="MSMLPEAKLDILLAHHASLEAQLLGQVNSENYVRITRELAELNP
                     LIDSVKAYRAANTELAGIDALIADPATEPEMRGMAEAERETLAARSVELAQQIRVALL
                     PKDAMDDRNVVLEIRAGTGGDEAALFAGDLFRMYERFASLQGWKVEVISASEGTMGGF
                     KEIIAEVRGRGAFAKLKFESGVHRVQRVPDTETQGRIHTSAATVAVLPEVEEVDVDIK
                     SDDLRIETMRSQGAGGQHVNKTESAIRITHIPTGIVVSMQDSRSQHKNRASAMNILRS
                     RIYDAERQRIDAARSADRREKVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLPQVIA
                     GESLSELVDALTTEHQAAQLAAQGAAA"
     gene            1627..2508
                     /gene="prmC"
                     /locus_tag="VN968_23265"
     CDS             1627..2508
                     /gene="prmC"
                     /locus_tag="VN968_23265"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HXL32187.1"
                     /translation="MTDCCAGQTVGTARRRLAARFKSGAIDSPELDARILVGAALGLD
                     LTGMIAAATRAFTAAEAARLEDFAGRRLKGEPVARILGSREFWGLPLQLSAATLVPRP
                     DTETVVALALQMLRAGPGADRPRIADLGTGSGAILLALLSELPDAVGVGTDISAAALR
                     TASANARNLGLGRRAAFVACDYASALSGAFDLIVSNPPYIRSAEINDLASEVRDHDPL
                     GALDGGSDGLDAYRALVPQAVRLLASGGGLALEVGHDQGADVKQLMAAAGLTLCGPPR
                     TDLAGIPRAVAGRKLPS"
     gene            complement(2605..2913)
                     /locus_tag="VN968_23270"
     CDS             complement(2605..2913)
                     /locus_tag="VN968_23270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32188.1"
                     /translation="MRYAHRIAAQLKSTQILPYVAVSKDAFPQQSSAHAAGGTKSFSL
                     PKQAKVSCVSFQAQYQAFVHFAVGSGAAFKSHRAQVDLRSGTFHSGRASRFENSGLHP
                     "
     gene            3041..3766
                     /locus_tag="VN968_23275"
     CDS             3041..3766
                     /locus_tag="VN968_23275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HXL32189.1"
                     /translation="MRNGQNNNKRMRNRNNNNNNNNNNNRRGQNPMTRVFESNGPDIK
                     IRGTASHVAEKYVQLARDARSSGDPVAAENYYQHAEHYFRLIAAAQEQFRQNQPQPRT
                     ENEMPSEDGDDESESFSNFGQEPGFVPAQPQPFVPRDNNPREAQPYQPREQHPPREHR
                     PQPQFQPPQPQPQPVIPDSVSVDRLPSFITGPQPQINGGPGGFEANGGERFPRRRRRP
                     HGPRPEGMPAPVTPSDDFNPGNE"
     gene            complement(3821..4564)
                     /locus_tag="VN968_23280"
     CDS             complement(3821..4564)
                     /locus_tag="VN968_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXL32190.1"
                     /translation="MTIDVIDLRDFYSQRLGIVARRLINRGIHARWPNAEGQRVLGLG
                     YPTPYLGLFREDSERCIAFMPAAQGVLKWPTARPALATLVDEFSLPLSDAAVDRILMV
                     HSLEMSDDPEGLLREMWRVLAPSGRLIAVIPNRRGVWTRTDTTPFGHGRPYSRSQITQ
                     LLRQTWFTPTAWGEALFMPPVGGGWFLRSAMAWERIGAALSLPFAGVHIVEATKQVYR
                     AIPALRERPRLIPALQPVLVPSSLQRDDV"
     gene            4697..5464
                     /gene="gloB"
                     /locus_tag="VN968_23285"
     CDS             4697..5464
                     /gene="gloB"
                     /locus_tag="VN968_23285"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HXL32191.1"
                     /translation="MAAEIRLFPCLTDNFGYLIHDPATGATASIDAPEAAPIAKALER
                     EGWTLTDILVTHHHGDHVGGIPELKQKFGCRVVAPHEKSAKIANVDLRVGQGDVVKIG
                     DLLARVLETPGHTLDHISYVFDNEKALFAADTLFSIGCGRVFEGTYPMMWDSLLKLRA
                     LPDDFKLYCGHEYTAANVKFALTVEPDNAALKARAAEVTRLRADSKPTVPTLLGEEKK
                     ANVFLRADEPSVAASVRMKGRSAAEVFGELRERKNKS"
     gene            5464..5898
                     /locus_tag="VN968_23290"
     CDS             5464..5898
                     /locus_tag="VN968_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL32192.1"
                     /translation="MVRPPTAADIIARLELRPHPEGGHYRETFRDPRVDGNGRAVSTA
                     IYFLLARGERSHWHRIDAVEAWHYYAGSALVLHIANDGGQRIVRLGPDLAAGEVPQAI
                     VPAQAWQAAESSGDWTLVGCTVAPGFEFAAFELAEPDWFPGR"
     gene            5974..6669
                     /locus_tag="VN968_23295"
     CDS             5974..6669
                     /locus_tag="VN968_23295"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXL32193.1"
                     /translation="MLSTIDVTDLAGSQVIEPAILYFGTPVVLIGSTNEDGSANLAPM
                     SSAWWVGWRCMLGLASNSKTTENMIRAGECVLNLPSADLVDAVNNLARTTGSNPVPAG
                     KIRRGYRHEKDKFEISGLTRFAGETVAAPRAAECPVQIEAKVAQVHEMAQEDEVWRGH
                     LVAIEVRVTRVHAHPGIMMDRQPDRIDPDKWRPLIMSFQQFYGLTPQRLQRSELGQIP
                     ESAYKPPGWRPAA"
     gene            complement(6811..7389)
                     /locus_tag="VN968_23300"
                     /pseudo
     CDS             complement(6811..7389)
                     /locus_tag="VN968_23300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199548.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(7658..>7877)
                     /locus_tag="VN968_23305"
     CDS             complement(7658..>7877)
                     /locus_tag="VN968_23305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL32194.1"
                     /translation="VTVNAICPGYINTEMVQAVPKEVLEKSILPLIPVGRLGEPDEIA
                     RAVVFLAADDAGAITGSTLTINGGQYMV"
BASE COUNT         1544 a         2534 c         2471 g         1328 t
ORIGIN      
        1 gcatggccat gcgctgccgg aacgcatcga cgtcggcacc atggtcgaag tgccggcgct
       61 gctctatcag ctcgacgaac tcctgaagaa agtcgatttc atctcggtcg gatccaacga
      121 cctgtttcag ttcctgttcg cggtcgatcg cggcaatgcc aaggtttcgg agcgcttcga
      181 caccatgtcg gccccgatcc tgcgggcgtt gcgcgagatc gtgcggaagg cgaatgccgc
      241 gaagaaatca gcctcgctgt gcggcgagat ggccgcaaag ccgctcggtg cgctggcgct
      301 gatcgcattg ggctaccgct cgctgtcgat gtccgccacc gggcatggtc cggtcaaggc
      361 gatgatcctc gatctcgacg ccaagaaggc cgaggcgatg atggcgccgc tgctcgatgc
      421 gccgtccggc agcgtgtcga tccgggaaaa gctgacggag ttcgccgagg ctgaggggct
      481 gtcgttgtag cggcgttctg cccgcacaac gccctctgcg catcccgctt tccgagatat
      541 actgatgtcc atgctccccg aagccaaact tgatatcctg ctggcgcacc atgcctcgct
      601 cgaggcgcag ttgctcggcc aggtgaattc cgagaactac gtgcggatca cgcgcgagct
      661 cgccgagctc aatccgctga tcgactcagt gaaagcctat cgcgccgcca ataccgagct
      721 tgccggcatc gatgcgttga tcgccgatcc cgcaaccgaa cccgagatgc gcggcatggc
      781 ggaagccgag cgcgaaacgc ttgccgcgcg cagcgtcgag ctcgcccagc agatccgcgt
      841 ggcgctgttg cccaaggacg ccatggacga tcgcaacgtc gtcctggaaa tccgcgccgg
      901 caccggcggc gacgaggccg ccttgttcgc cggcgacctg ttccggatgt atgagcgttt
      961 tgcaagcttg cagggctgga aggtcgaggt gatctccgcc agcgaaggca ccatgggcgg
     1021 cttcaaggaa atcatcgccg aggtgcgtgg ccgcggcgca tttgcaaagc tgaaattcga
     1081 atccggcgtg caccgggtgc agcgcgtgcc cgacaccgag acgcaagggc gcattcacac
     1141 ctcggcggcg acggttgccg tgctgccgga agtcgaggaa gtcgatgtcg acatcaagag
     1201 cgacgatttg cggatcgaga ccatgcgctc gcaaggcgcc ggcggccagc atgtcaacaa
     1261 gaccgaatcc gcgatccgca tcacccatat tccgaccggc atcgtcgtca gcatgcagga
     1321 cagccgatcc cagcacaaga accgcgcgtc ggcgatgaac atcctgcgct cgcgaatcta
     1381 cgacgccgag cgccagcgca tcgatgccgc gcgctcggcg gaccgccgcg agaaggtcgg
     1441 ctccggcgat cgctccgagc gcattcgtac ctataatttt ccgcaaggcc gcgtcaccga
     1501 tcatcgcatc aacctcacgc tctacaagct tccgcaggtg atcgcgggcg aaagcttaag
     1561 cgaactggtc gacgcgctga cgaccgagca tcaggcagca caactcgccg cccagggcgc
     1621 cgcggcgtga ccgattgctg cgctggacaa actgtcggga ccgcgcggcg caggctggcg
     1681 gcgcggttca aatctggagc catcgattcg cccgaactcg acgcgcggat tctggtcggg
     1741 gcggcgctcg gtctcgattt gaccggcatg atcgcggccg cgacgcgggc tttcaccgcc
     1801 gctgaggccg cgcggctcga ggattttgca ggccgccgcc tcaagggcga gccggtcgcg
     1861 cgcattctcg gctccaggga attctgggga ctgccattgc aactctcggc cgcgacgctg
     1921 gtgccgaggc ccgataccga aacggtggtc gcattggcgt tgcaaatgct gcgcgccggc
     1981 cctggtgccg atcgcccacg catcgccgac ctcggcaccg gctcaggtgc gattttactg
     2041 gcgctgctgt ccgaactgcc tgatgcggtg ggggtcggca ccgacatcag cgcggcggcg
     2101 ttacgaaccg caagcgccaa tgcccgcaat ctgggattag gccgtcgcgc ggcgttcgtc
     2161 gcctgcgact atgcctccgc gctgtcgggg gcgttcgatc tgatcgtatc gaatccgccc
     2221 tatatccgct ccgccgagat caatgatcta gcaagcgagg tccgcgatca cgacccgctc
     2281 ggcgcgctcg acggcggcag cgacggactt gacgcctacc gcgcactagt tccgcaggcg
     2341 gtgcggctgc tggcgtctgg cggcggcctc gctttggagg tcggacatga tcaaggcgcc
     2401 gatgtgaagc agctgatggc ggcggcaggg ttaacgcttt gcgggcctcc ccgaaccgac
     2461 ctggccggca tcccacgggc tgtcgcgggc cggaaattgc cctcataaag cccgattggg
     2521 acacaaaaaa tcacttggaa tattccccgg gagcgactac gttccgccta caacatcggt
     2581 ccagggttgc tggccccgta gatgctacgg gtgaaggcca gagttctcaa agcgagaagc
     2641 ccgccccgag tgaaaggttc cagaacgcag gtcgacttga gcgcgatggc ttttgaaagc
     2701 tgcgccgctt ccgaccgcaa agtgaacgaa agcctgatat tgcgcttgaa aactcacgca
     2761 agaaaccttt gcttgttttg gcaggctgaa tgatttggtg ccaccagcgg cgtgtgcgct
     2821 ggactgctgg ggaaacgcgt ctttgctgac cgcgacatac ggcaaaatct gtgtggactt
     2881 gagctgcgct gcgattcggt gcgcgtaacg catgccggat cgggcggagc gacctgacga
     2941 agcgactatg acaacggcac tccaagattc gtaactttaa gattcgtaac ttttcgtgac
     3001 ttcaaggcta acaacttcag ggctggaata aaggcgagac atgagaaacg gtcagaacaa
     3061 taacaagcgg atgcgcaacc ggaataacaa taacaataat aataacaaca ataaccggcg
     3121 cggccagaat ccgatgacgc gggtgttcga atcgaacgga cccgacatca agatccgcgg
     3181 caccgcatcc cacgtcgccg agaagtacgt gcagctggcg cgcgacgcgc gctcttccgg
     3241 cgatccggtg gccgccgaga attactacca gcatgccgag cattatttcc gcttgattgc
     3301 cgctgcccag gaacagttcc gtcagaatca gccgcaaccg cgcaccgaga acgaaatgcc
     3361 ttcggaggac ggcgacgacg agagcgaaag cttttcgaat ttcgggcagg agcccggctt
     3421 cgttccggcg cagccgcagc cgttcgtacc gcgcgacaac aatccgcgcg aggcccaacc
     3481 ctaccagccg cgcgagcagc atccgccgcg cgagcatcgc ccgcagcctc agtttcaacc
     3541 gccgcagccc cagccacagc cggtgattcc cgacagcgtc agcgtcgatc gcctgccgtc
     3601 gttcatcacc ggaccgcagc cgcagatcaa cggcgggcct ggcggtttcg aggccaatgg
     3661 cggcgaacgt ttcccgcggc ggcggcgccg gccgcatggc ccgcgccccg aaggcatgcc
     3721 cgcgccggtg accccgagtg acgatttcaa tccagggaat gagtaagaca accctcagcg
     3781 tcgtcgtccc ggcgaagcgc cgggacgacg tacagattgg ttagacatca tcccgctgca
     3841 gggacgacgg caccagcacc ggctgcagcg ccggaatgag gcgcgggcgt tcgcggagcg
     3901 ccgggatcgc ccggtagacc tgcttggtgg cttccacgat atgaacgccg gcgaacggca
     3961 gcgatagcgc ggcaccgatg cgctcccatg ccatcgcgga gcgcaggaac cagccgcccc
     4021 cgaccggcgg catgaacagc gcctcgcccc acgccgtcgg cgtgaaccag gtctgccgca
     4081 gcaattgcgt gatctgcgag cgcgaatagg gccggccgtg gccgaacggc gtggtgtcgg
     4141 tgcgcgtcca caccccgcgc cggttcggaa tgacagcgat caatcggcca gagggtgcca
     4201 gcacccgcca catctcgcgc agcagtccct cgggatcgtc ggacatttcc agagagtgaa
     4261 ccatcaggat gcggtcgacg gcggcgtccg acagcggcag cgaaaattca tcgaccaggg
     4321 ttgccagcgc gggccgcgcg gtcggccatt tcaggacacc ctgcgccgcc ggcatgaagg
     4381 cgatgcaacg ctcgctatct tcgcgaaaaa gcccgagata gggcgtggga tagccgaggc
     4441 cgagtacccg ctggccttcc gcgttgggcc agcgcgcgtg aatgccacga ttgatcagcc
     4501 gccgcgccac gatgccgagg cgctgcgaat agaagtcgcg gagatcgatg acatcgatgg
     4561 tcatgaactt aatctaacac gtcgccgcgt cgcgcggagc gccgaatcac gcattgcctg
     4621 gtaagcgtta acgccatatt tccccgccat acggggctgt gctagcctca ccgcctgtct
     4681 ggaacatgga gatatcatgg ccgctgaaat ccgcttgttc ccctgcctga ccgacaattt
     4741 cggttacctg atccatgatc ccgcgaccgg ggcgaccgct tcgatcgacg ctccggaagc
     4801 cgcgcccatc gccaaggcgc tggagcgcga gggctggacg ctgacggata ttctggtcac
     4861 ccatcatcac ggcgatcatg tcggcggcat tcccgagctg aaacagaaat tcggatgccg
     4921 cgtggtcgcg ccccacgaaa agtcggccaa gattgccaac gtcgatctgc gcgtcggtca
     4981 aggcgacgtc gtgaagatcg gagacctcct ggcgcgggtg ctggagacac cgggccacac
     5041 tctcgatcac atcagctacg tgttcgacaa cgagaaggcg ctgttcgccg ccgacacatt
     5101 gttctcgatc ggctgcggac gggtgttcga aggcacctat ccgatgatgt gggactcgct
     5161 gttgaagttg cgcgccctgc ccgatgattt caaactctat tgcggccatg aatacaccgc
     5221 ggccaacgtc aagtttgcgc tgaccgtcga gccggacaat gccgcgctga aggcacgcgc
     5281 ggcggaagtg acgcggttgc gcgcagacag caagccgacg gtcccgacgc tgcttggcga
     5341 agagaagaag gccaatgtgt tcctgcgcgc cgacgaaccg tcggtcgcgg ccagcgtgcg
     5401 gatgaagggc aggagcgccg cggaggtgtt tggcgaattg cgcgaacgca agaacaagtc
     5461 ctgatggtcc ggccccctac cgccgccgac atcattgcgc ggcttgagtt gaggccgcac
     5521 ccggagggtg ggcattatcg cgaaacattt cgcgatccgc gcgtcgatgg caatggacgc
     5581 gcggtctcca ccgcgatcta tttcctgctg gcgcgcggag aacgctcgca ctggcaccgt
     5641 atcgatgcgg tcgaggcctg gcattattac gccggcagcg cacttgtcct gcacattgcc
     5701 aatgatggcg gccagcgcat cgtcaggttg gggcccgatc tcgccgcggg cgaagtaccg
     5761 caggccatcg tgccagcgca ggcgtggcag gctgccgaga gcagcggcga ctggacgctg
     5821 gtcggatgca ccgttgcacc gggattcgag tttgcggctt tcgaattggc cgagcctgat
     5881 tggttccccg gccgctgaaa aatccattcg ggaatcgggt gggtgtgtcc gcaccaactc
     5941 actacgtttg tggcttcaca tgaggaagcc gacatgctca gcacaatcga tgtcaccgat
     6001 cttgcaggtt cgcaggtgat cgagccggcg atcctgtatt tcgggacgcc agtggtcctg
     6061 atcggctcga ccaacgaaga cggttctgcc aacctcgcgc cgatgtcgtc ggcctggtgg
     6121 gtcggctggc gctgcatgct gggactggca agcaactcca aaaccaccga aaacatgatc
     6181 cgcgccggcg agtgcgtgct caaccttccc tcggcggatc tcgtcgacgc cgtcaacaat
     6241 ctggcgcgca ccaccggctc gaatcccgtg cccgcgggaa aaatcaggcg cggctaccgg
     6301 cacgagaagg acaaattcga aatcagcggc ctgacgcgat tcgcaggcga gacggttgcc
     6361 gcgccgcgcg ccgcggaatg cccggtgcag atcgaggcaa aggtcgcgca ggtccatgaa
     6421 atggcgcagg aggacgaagt ctggcgcggc catctcgtgg cgatcgaggt tcgcgtcacc
     6481 cgcgtgcacg cgcatccggg aatcatgatg gaccgccaac ccgatcgcat cgatcccgac
     6541 aaatggcggc cgctgatcat gagctttcag cagttctacg gcctcacgcc gcaaaggctg
     6601 cagcgctccg aactcggaca aattccggag agcgcctaca agccaccggg ctggcggccg
     6661 gcggcttgac gagccgttcg tcgttccggg atgcgccatc tttggcgcag ccccggaatc
     6721 catactcctt gcagcggtta tggattccgg gctcgctcgt ttcactcgcg ccccggaatg
     6781 acggtcatta ttgttgatga gccaaccgac ctaagctgaa cgcttcacca tcatatcctt
     6841 cgccgcgatc aagccaccgc cggcgatcag caccgcggcg atggcgatgt tcgcactcgg
     6901 ctttgcaaag cccgccagaa tcaagaacgc ggtcgagagc agcggcgtcg cgtaggacgc
     6961 agcacccagc acgcggatgt cgccgcgctt cattccgata tcccaggcat aaaacgctgc
     7021 gccgaccggt ccgacaccga gcgccgcggt cgccagccat tgcgcccaag tatcgggcca
     7081 gaccgtgacc tcgaccagcc catgccccgc tgctgcgagc agcgccgtgg cgagacaaaa
     7141 gcccgccacc gcgtccgttg gcaccgattt gagcttgcgc gacatcactg aataggccgc
     7201 ccacacgaag gctgcgacaa acgccgcgat cagcccgggc acttgtcccg gcgcgaaccc
     7261 accgccgcag ttgcctgcaa acaaaagcac cgtgccggcg agcccgagca gcgcaccaat
     7321 gatgtgatgc ggcgccagcc gctcgctggg cagcagcgac gaaaacagca cgatcagcag
     7381 cgaccacatc aggatcgcgg tcaggccgat cagcgtggca gtgcgtgagg tcatttcgct
     7441 tagcgatgtc gaagtgcttt tccgtcatgg ccgggcatag ccgtccgaag gacggcgtcg
     7501 cttccgctcg cctatgccgg ccatccacgt cttcctcatt gtttgacttt gaagacgtgg
     7561 atgcccggca gcgcagacaa gtttacgtag tctgcgcaag gcagactact atcgccgggc
     7621 atgacgactc attggaaatc atcgccaaac gcaacaatca gaccatatac tgtccgccgt
     7681 tgatcgtgag cgtcgagcca gtaatggcgc cggcgtcgtc ggcggcgagg aacacaacgg
     7741 cgcgggcgat ctcgtcgggc tcgccgaggc gacccaccgg gatcagcggc aggatgcttt
     7801 tctccagcac ctccttcggc accgcctgca ccatctcggt gttgatatag ccggggcaga
     7861 tcgcattcac cgtgacg
//
LOCUS       DATNOI010000577         3090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_703305,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000577 DATNOI010000000
VERSION     DATNOI010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703305"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VN968_23310"
     CDS             complement(<1..383)
                     /locus_tag="VN968_23310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HXL32195.1"
                     /translation="MNPLQIKLVQESFTKVAPISDQAAVMFYSRLFEVAPQVKAMFPA
                     DMTEQRRKLMAMLSVVVSGLSSLETILPAASALATRHVSYGAKAEHYPVVGGALLWTL
                     EKGLGDGWTTEVADAWTAAYGTLSG"
     gene            complement(471..1265)
                     /locus_tag="VN968_23315"
     CDS             complement(471..1265)
                     /locus_tag="VN968_23315"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32196.1"
                     /translation="MQAYLKLDHIDKTFTRGNASTEVLKDINLTIEKGEYVSIIGHSG
                     CGKSTLLNIVAGLTNATMGCVLLENREVNSPGPDRAVVFQNHSLLPWLTVYENVRLGV
                     DKVFASSKTRSERDAWTMHNLNLVQMAHAKDKRPAEISGGMKQRVGIARALAMEPKVL
                     LLDEPFGALDALTRAHLQDSVMALHQKLGNTILMITHDVDEAVLLSDRIVMMTNGPSA
                     RIGEVLDVPLARPRKRLELSTNAAYLKCRLRVLEFLYERHRFVEAA"
     gene            complement(1277..2170)
                     /gene="ntrB"
                     /locus_tag="VN968_23320"
     CDS             complement(1277..2170)
                     /gene="ntrB"
                     /locus_tag="VN968_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HXL32197.1"
                     /translation="MNMPAMKTEPVTIRPATAAPVVAMTPKRPPRAEKYIRMARETAV
                     RVVPPLIVLALLMLFWELACRRAGSTLPPPSRVYKDTKELIFDPFFDRGGIDKGLFWH
                     LSASLQRVALGYSLAAVAGVALGTLVGQSVWAMRGLDPLFQVLRTIPPLAWLPLSLAA
                     FRDGQPSAIFVIFITSIWPIIINTAVGIRNIPQDYRNVAAVVQLNPVEFFTKIMIPAA
                     APYIFTGLRIGIGLSWLAIVAAEMLIGGVGIGFFIWDAWNSSHISEIILALFYVGIIG
                     FVLDRLIAGLAKIVTRGTAMN"
     gene            complement(2232..>3090)
                     /locus_tag="VN968_23325"
     CDS             complement(2232..>3090)
                     /locus_tag="VN968_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HXL32198.1"
                     /translation="DLKIGLDTAPFKPALEKKKASGKSIKAAMTFPGGTHDLWIRYWL
                     AAGGIDPDKDIETIVVPPPQMVANMKVGTMDCFCVCEPWNLQLIHQEIGYTAITTGEL
                     WNKHPEKSFGMRAAYVDKYPKAAKALLMAVMEAQQWCEKPDNREEVAAICAKRQWINC
                     PVDDVTDRVKGKFDYGTGKVVENSPHIMRFWADNASYPYQSHDLWFLTEDIRWGKYEA
                     GYDTKALIAKVNREDLWKEAAKDLGVAASDIPASRSRGKETFFDGKVFDPENPAAYLK
                     SLTIKRVDV"
BASE COUNT          596 a          942 c          943 g          609 t
ORIGIN      
        1 aagccggaca gcgtcccata ggccgcggtc catgcgtccg cgacttccgt cgtccagcca
       61 tcgccaagtc ccttctccag cgtccacaac agagcgccgc cgacgacggg atagtgctcc
      121 gctttcgcgc cgtagctgac gtggcgcgtt gccagcgcgc tcgcggccgg cagtatggtt
      181 tcaagactgc tgagtccgct caccacgacg gaaagcatcg ccatcaattt cctgcgctgt
      241 tcggtcatat cggcgggaaa catcgccttg acctgcggcg cgacctcgaa cagacggctg
      301 taaaacatta ccgccgcctg atccgaaatc ggcgcgacct tggtgaagct ctcttgaacc
      361 agtttgattt gtaacggatt cattgcaact ctccatgcga gaacgtgaac aggttggtcg
      421 ccacgtataa aaataggttc aacggtaaag cgtcggaaac ggataagagc ttaagcagcc
      481 tcgacaaaac gatgccgctc gtagaggaat tccagcacgc gcagccggca tttgaggtag
      541 gcggcattgg tcgagagctc gagccgcttg cgcgggcgcg ccagcggcac gtcaaggact
      601 tcgccgatcc gcgcgctcgg tccattggtc atcatcacga tgcggtccga caacagcacc
      661 gcctcatcga catcgtgggt gatcatcaga atggtgttgc cgagcttctg atgcagcgcc
      721 atcaccgaat cctgcagatg cgcgcgggtc agtgcgtcga gcgcgccgaa cggttcgtcc
      781 agcagcagca ccttgggctc catcgccagc gcgcgcgcga tgccgacgcg ctgcttcatg
      841 ccgccggaga tttccgccgg gcgcttgtcc ttggcgtggg ccatctgcac gagattgaga
      901 ttatgcatgg tccatgcgtc gcgctcgctg cgggttttgc tcgatgcaaa aaccttgtcg
      961 acgccaagac gcacgttttc ataaaccgtg agccagggca gcaggctgtg gttctgaaac
     1021 accaccgcgc gatccggacc cggcgaattg acttcgcggt tttccaggag cacgcagccc
     1081 atcgtggcgt tggtgagacc ggcgacgatg ttgagcagcg ttgacttgcc gcagccggaa
     1141 tgtccgataa tcgagacgta ctcacccttc tcgattgtca ggttgatgtc cttgagcacc
     1201 tcggtcgagg cgttgccgcg ggtgaaggtc ttgtcgatgt ggtcgagttt cagataggcc
     1261 tgcatcccat gttccttcaa ttcatcgcgg tgccgcgggt tacgatcttc gcaagtcccg
     1321 cgatcaggcg gtcaagcacg aagccgatga tgccgacgta aaacagcgcc agaatgattt
     1381 cgctgatgtg cgaggaattc caggcgtccc agatgaagaa gccgatgccg accccgccga
     1441 tcagcatctc cgccgccacg atcgcaagcc aggacaggcc gatgccgatc cgcaagccgg
     1501 tgaagatgta gggcgccgcc gccgggatca tgatcttcgt aaagaattcc accggattga
     1561 gttgaaccac cgctgcgaca ttgcggtagt cctgcgggat gttgcggatg ccgaccgcag
     1621 tgttgatgat gatcggccag atcgaggtga tgaaaatcac gaagatcgcc gagggctggc
     1681 cgtcacgaaa tgccgccagc gaaagcggca gccacgccag cggtggaatg gtgcgaagca
     1741 cctgaaacag cggatcgagc ccgcgcatcg cccagaccga ttgtccgacc agcgtaccga
     1801 gcgcgacacc cgcgaccgcc gcgagcgaat agcctaacgc gacgcgctga aggctggcgg
     1861 acagatgcca gaacagtccc ttgtcgatgc cgccgcgatc gaagaacgga tcgaagatca
     1921 gctccttggt atccttgtag acccgcgacg gcggcggcag cgtcgaaccg gcgcggcggc
     1981 aggccagttc ccagaacagc atcagcagcg ccagcacgat caagggcggc accacccgca
     2041 ccgcggtctc gcgcgccatc ctgatatatt tttcggcgcg cggcggtcgc ttcggcgtca
     2101 tcgcgacgac cggcgccgcg gtcgccgggc ggatcgtgac gggttcggtc ttcattgcag
     2161 gcatattcat cgaaagtgta tctccgtgtg caatggatag ggccgcacgc gcgcgaacgg
     2221 tctcgcctcg gtcagacgtc aacacgcttg atggtaaggg acttcagata cgccgccgga
     2281 ttttccggat cgaacacctt gccgtcgaag aaggtctcct tgccgcgcga cctggaggcc
     2341 gggatatcgg atgcggcgac gccgagatcc tttgcggctt ccttccagag gtcctcgcgg
     2401 ttgaccttgg cgatcagcgc cttggtgtcg tagcccgctt catatttgcc ccagcggata
     2461 tcctcagtga ggaaccacag atcgtggctc tgataggggt aagatgcgtt gtcggcccag
     2521 aaccgcatga tgtgcgggct gttctcgacc accttgccgg tgccgtagtc gaacttgccc
     2581 ttgacgcgat cggtgacgtc atcgacgggg cagttgatcc actggcgctt ggcgcagatc
     2641 gccgcgactt cctcgcggtt atcgggcttc tcgcaccact gctgcgcctc catcacagcc
     2701 atcagcagcg ccttcgctgc cttcgggtat ttgtcgacat aggcggcgcg cataccgaag
     2761 gatttttcgg gatgcttgtt ccagagttcg cccgtcgtga tcgcggtata gccaatctcc
     2821 tgatggatca attgcaggtt ccagggctcg cacacgcaga agcaatccat ggtgccgacc
     2881 ttcatgttgg cgaccatctg cggcggcggc accacgattg tttcgatgtc cttgtccgga
     2941 tcgatgccgc cggcggcgag ccagtaacga atccacagat cgtgggtgcc gcccgggaat
     3001 gtcatcgcgg ccttgatcga tttgccggag gccttcttct tctccagtgc cggtttgaac
     3061 ggcgcggtgt cgaggccgat cttgaggtcg
//
LOCUS       DATNOI010000578        11306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_949577,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000578 DATNOI010000000
VERSION     DATNOI010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11306
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949577"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VN968_23330"
     CDS             <1..447
                     /locus_tag="VN968_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HXL32199.1"
                     /translation="LYLEALTLVERLHRRLLDVIKDEFDRRGRADINSVQALLLYNIG
                     DKELTAGELRTRGYYLGSNVSYNLKKLVELGFLDHQRSRVDRRSVRIRLTAQGQEIRR
                     IVDALYQKHVKTVEQVGGISNEEFATLNKSLHRLERFWTDQILYRL"
     gene            645..866
                     /locus_tag="VN968_23335"
     CDS             645..866
                     /locus_tag="VN968_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32200.1"
                     /translation="MLVERGVRVMSVEVVGDAYAIASNYFRRTGAISDSFVTDERLLQ
                     IIVTLFQRGECNKIKLANKAITQFESELV"
     gene            901..1122
                     /locus_tag="VN968_23340"
     CDS             901..1122
                     /locus_tag="VN968_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32201.1"
                     /translation="MEAAIDRIMQTYDLLTNRRAAANAEARAKVTEYVTTLFEAGERD
                     LHRLTVCGLTYLRELDGSNDPVKAGFTGL"
     gene            1338..1975
                     /locus_tag="VN968_23345"
                     /pseudo
     CDS             1338..1975
                     /locus_tag="VN968_23345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604121.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
     gene            2300..3607
                     /gene="glyA"
                     /locus_tag="VN968_23350"
     CDS             2300..3607
                     /gene="glyA"
                     /locus_tag="VN968_23350"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HXL32202.1"
                     /translation="MSSSSSAQTASAPDSFFTATLDRADPDIAAAIRGELGRQRHEIE
                     LIASENIVSRAVLEAQGSVLTNKYAEGYPGNRYYGGCEWVDVVETLAIERAKKLFGTQ
                     FANVQPNSGSQMNQAVFLALLQPGDTFMGLDLAAGGHLTHGAPVNISGKWFKAQHYTV
                     RRDDQVIDMDEVARRAEEVKPKLIIAGATAYSRSWDFKRFREIADGVGAYLMVDMAHF
                     AGLVAGGVHASPMPYAHVTTTTTHKSLRGPRGGLMLWNDEALTKKLNSAVFPGLQGGP
                     LMHVIAAKAVAFAEALRPDFKVYAKNVVENAKALAESLRANGLDIVSGGTDNHLMLVD
                     LRPKGLKGNVSEKALVRAAITCNKNGIPFDPETPFVTSGLRLGTPAATTRGFGVAEFK
                     QVAGMIAEVLNAIAQSQDGKAPLVESAIKERVRALTDRFPIYQ"
     gene            3620..4102
                     /gene="nrdR"
                     /locus_tag="VN968_23355"
     CDS             3620..4102
                     /gene="nrdR"
                     /locus_tag="VN968_23355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HXL32203.1"
                     /translation="MRCPSCNSLDTQVKDSRPTEDSAVIRRRRVCVACNFRFTTFERV
                     QLRELTVIKRNGRRVPFDRDKLVRSVQISLRKRPVDPERVEKMVSAIVRELESGGEAE
                     ISSEAIGEIVMEHLRQLDDVAYVRFASVYRNFREAKDFEAVLGELSGDDDARIALLRK
                     "
     gene            4099..5250
                     /gene="ribD"
                     /locus_tag="VN968_23360"
     CDS             4099..5250
                     /gene="ribD"
                     /locus_tag="VN968_23360"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HXL32204.1"
                     /translation="MIFRILEDRLAQKSRESKAADQRFMQLALTLGRRGQGRTWPNPA
                     VGAVVVKDGVIIGRGWTQPGGRPHAEPEALRRAGEAARGATLYVTLEPCSHIGKSPPC
                     ADAIIAAGIARVVSAIEDPNPEVAGQGHAKLRDAGISVDIGLGAAEAARDHAGHFRRV
                     RDKRPHVILKLAVSSDDKIGAAGRKPVAISGDAAKARVHLLRAQCDAILVGIGTVRAD
                     DPLLTCRLPGMAARSPVRVVLDRNLRIPGSSRLVHSARETPLWVMTSNLAEAPAAMKL
                     GAAGAQVIRVATATTPPGLDLAAVLRALSEKGITRLLVEGGARVASSFVAAGFVDEVW
                     LLRGPDPIGADGVAALGALPLTSITKSPNFRVRASKSLQKDTLTIYERT"
     gene            5251..5859
                     /locus_tag="VN968_23365"
     CDS             5251..5859
                     /locus_tag="VN968_23365"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HXL32205.1"
                     /translation="MFTGIITDIGEIVSLTPTAQGQLHRLRIACRYDRSTIADGASIA
                     CNGVCLTVVASGVAGDKTWFEVDAAAETLGMTTAKHWTRGTKLNLERALKIGDELGGH
                     IVAGHADGIATIVKRDDLPDMARFELRTTRELARFIAAKGSVTLDGVSLTVNTVQDVV
                     FSVLIIPHTLNVTTLGGWRAGAEVNLEVDLMARYAARLAEMK"
     gene            6041..6532
                     /gene="ribH"
                     /locus_tag="VN968_23370"
     CDS             6041..6532
                     /gene="ribH"
                     /locus_tag="VN968_23370"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HXL32206.1"
                     /translation="MADARRAQLKDQTDVSGARALIVEARFYDDIQDALLEGAVAELK
                     AAGVTHDVITVPGALEIPAAIAIALEAAEKRGRPYDAAIALGCVIRGDTIHFEIVSME
                     SSRALMDLAIAKRFPLGNGIITVNTDAQAWARARPSELNKGGDAARAALAMLRIKRRL
                     MRT"
     gene            6535..7056
                     /gene="nusB"
                     /locus_tag="VN968_23375"
     CDS             6535..7056
                     /gene="nusB"
                     /locus_tag="VN968_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXL32207.1"
                     /translation="MAENSKRQEKKPAKGPEKSKDKKANRRGAARLAAVQALYQMDIA
                     GAGLNDIFAEFESHWLGGEVEGDKYLPAEAAFFRDVVSGVVRDQAKLDPLIDDALSKG
                     WPLKRIDAILRAVLRAGSYELEHRKDVPGRVVVSEYVDVAHAFVDGEETGMVNAVLDQ
                     IARRFRGNEFSRG"
     gene            7204..8199
                     /gene="thiL"
                     /locus_tag="VN968_23380"
     CDS             7204..8199
                     /gene="thiL"
                     /locus_tag="VN968_23380"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HXL32208.1"
                     /translation="MTKSEQAPSGEDSLIARYFKPLATDPGAFNLGDDAAILNASGED
                     TVVTTDAIVEGVHFLADDPPDTVARKALRVNLSDLAAKGATPAGFVLTLALRAADDAW
                     LTLFARGLGSDAGLFGCPLLGGDTVSTPGPLTISITAFGRVPAGGMVRRSGAKPGDRV
                     VVTGTIGDAALGLDILKGGAAAALADAGAKAMLIGRYRVPQPRNALAKAVRDHAHAAI
                     DVSDGLAGDLAKLCGASGVSAVIDAPSIPLSAATAALLARGTVGIETIVSGGDDYEIL
                     CAIPEDSFEAFVQAAGQAGVAVTSIGMVVAGSSVPRFLDGEGAELALPRLSYSHF"
     gene            8407..10527
                     /locus_tag="VN968_23385"
     CDS             8407..10527
                     /locus_tag="VN968_23385"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HXL32209.1"
                     /translation="MTALWVIVLCGALSILYAIWATASVLKSDAGNPRMQEIAAAVRE
                     GAQAYLKRQYMTIGLVGVVIFALLAYFLGMLVAVGFAIGAVLSGAAGFIGMNVSVRAN
                     VRTAQAATTSLAGGLELAFKAGAITGMLVAGLALLGVTIYFAYLTQFQGLAANSRTVV
                     DALVALGFGASLISIFARLGGGIFTKGADVGGDLVGKVEAGIPEDDPRNPATIADNVG
                     DNVGDCAGMAADLFETYAVTTVATMVLAAIFFANSPLLVNMMTLPLAIGAACIITSII
                     GTFFVKLGASQSIMGALYKGLIATGILSLIGVAGVIYWLIGFGPLAGVKFTGMALFEC
                     GVVGLAVTGLIIWITEYYTGTENRPVKSIAASSVTGHGTNVIQGLAISMEATAAPALV
                     IIAGILITYSLAGLFGIAIATTTMLALAGMIVALDAFGPVTDNAGGIAEMAGLPKEVR
                     KATDALDAVGNTTKAVTKGYAIGSAGLGALVLFAAYNEDLKFFIANASKFPYFQGVQP
                     DFSLNNPYVVVGLLFGGLLPYLFGAMGMTAVGRAAGAIVEEVRRQFREKPGIMQGTDK
                     PDYGKAVDLLTRAAIKEMIIPSLLPVLSPIVVFFAIYFIAGGGPAGKSAAFSAVGAML
                     LGVIVTGLFVAISMTSGGGAWDNAKKYIEDGHYGGKGSEAHKAAVTGDTVGDPYKDTA
                     GPAVNPMIKITNIVALLLLAILAH"
     gene            complement(10578..>11306)
                     /locus_tag="VN968_23390"
     CDS             complement(10578..>11306)
                     /locus_tag="VN968_23390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXL32210.1"
                     /translation="RQGMWHFHKLTREGHLAARTLFRRACAIDPDLPESHIWLGRVSA
                     GIIAYGWSDAPSADSMEGVDAAARAITLDSRDPYAHYAFAIANSYSNAPVVAASAAEK
                     AIALSSSFALGHLVLGMARLFAGLAREAIEPLEHGLALNPNDPQNVVWYNVLAYAQLF
                     AGEAERALATANKVLAIRPTFRPTLETLVCCHVALGRLGDAHRCASRMNELAGPESHF
                     LAPLKQLHPEWDQRISQMLDQART"
BASE COUNT         2038 a         3570 c         3638 g         2060 t
ORIGIN      
        1 ctctatctcg aagcgttgac tttggtggag cggctgcatc gccgcctgct cgacgtcatc
       61 aaggacgaat tcgaccgccg cggccgcgcc gacatcaatt cggtgcaggc gctgttgctc
      121 tataacatcg gcgacaagga actgaccgcg ggcgaattgc gcacgcgcgg ctattacctc
      181 ggctccaacg tctcctacaa tctgaagaag ctggtcgagc tcggcttcct cgatcatcag
      241 cgctcgcgcg tcgatcgccg ctcggtccgc attcgcctca ccgcgcaggg ccaggaaatc
      301 cgccgcatcg tcgacgcgct gtatcagaag cacgtcaaga ctgtggagca ggtcggcggc
      361 atctccaacg aggaattcgc gaccctgaac aagtcgctgc accggctcga gcggttctgg
      421 accgaccaga tcctgtatcg gctctgagac tttcttccgc cgcgacaaaa aggccaagcc
      481 ggcgttgaaa agaccggcag ccagcagaaa agacaacttc cccaagcgca tcggtccggt
      541 ttccggcccg gtgcgtttcg ttttggcggc caaaaacagc cttctgcagc ggaaccagtg
      601 cccgggttcg cgctttaata atccgtatcg gaaggattga cgtcatgctg gtcgagcgtg
      661 gggtgcgggt tatgagcgtg gaggtggtag gcgacgccta tgccatcgcc tcgaactatt
      721 ttcgccgcac cggcgccatc agcgacagct ttgtcaccga cgaacggctg cttcaaatta
      781 tcgtcacgtt gtttcaacgc ggcgaatgca acaagatcaa gctcgccaac aaggcgatca
      841 cgcaattcga atccgagctg gtttgagcga tagctgcctg atcaacagcg aaagaaatcc
      901 atggaagctg ccatcgaccg catcatgcag acctatgacc tgctgacaaa ccgccgtgcc
      961 gccgccaatg cggaggcccg cgccaaggtg acggagtatg tcacgacgct gttcgaggcc
     1021 ggtgaaaggg acctgcaccg cttgacggta tgcgggctga cctacttgcg cgaactcgac
     1081 ggctcaaacg atccggtgaa agctggattt accgggctgt aggggcgagg cctctgacgc
     1141 gagaagtctg ctctgcttgt catgccccgc gaaagcgggg catccagtac gccgcggcct
     1201 ttcgattcaa tcaccagctt ctgtggaata ctggatggcc cggtcaagcc gggttgcgac
     1261 aaccgcttcg caagcttgct gccctttttc aaagacgtct gatacagctt ggacaacaat
     1321 cacgccgagg aaacaccatg ctcacgctgc atcatctcaa cgactcccgc tcgcagcgga
     1381 ttctgtggct gctggaagaa ctgggggtgc cctacgagat gaggcgctat cagggcaacc
     1441 cggagacgcg cctggcgccg ccggagttga aggcggtgca tccgctcggc aagtcgccgg
     1501 ttatcaccga cggcgacatc agaatcgcgg agtccggcgc catcgtcgat tacgtcatcc
     1561 gccgccacgg cctaggaaac ggtcaaccgg caatgatgcc cgccccaggc agcgccgatt
     1621 acgaggtcta taacgagtgg ctgcattatt cggaaggttc tgcgatgctg ccgctgctgc
     1681 tcaacctcta tgtttccagg ttgaaggagg cgggcgcgcc gctttatccg cgcatcgaca
     1741 gcgagatggc caaccacctc ggctatgtcg atggcgcgct gaagggccgc gaatactttg
     1801 tcggcaattc gctcaccggc gccgatatcc agatgagctt tgtcggcgac atggcgaaag
     1861 cgttcaacaa gctcggaccc tatccgaacc tcagcgcctg gctgtcgcgg atgcacgccc
     1921 ggcccgcatt cagcgctcaa tcgagaaggg cgggacgtac aggtttgcga agtaggagcg
     1981 gccgtcgtcc cggccaagtg agcgcagcga acgcgagccg tgacccatac gccgtatcgc
     2041 tcggaacggg cacgctgtca gacggcttat tccgccaact cccgctgggg gttatgggcc
     2101 ccggctccag gccggggcga cgtgggaagg gccttgggga ctccctatcc ccaccatatc
     2161 ttgcataaat actgggcgtg gaccgcaagc gcttggccat tccggcccaa tgtggtatcc
     2221 cgccagcaca gcttcgaaac ctttcccatg accgaccgca atgtccgtaa caggttgcgg
     2281 cggtggagat atttcgccca tgagctcttc ctccagcgcc caaacggcct ccgcgcccga
     2341 ctcgttcttc accgccacgc ttgaccgggc cgatccggat atcgcggccg cgatccgtgg
     2401 cgagctcggc cgccagcgcc acgagatcga gctgatcgcc tcggaaaaca tcgtcagccg
     2461 cgccgtgctg gaagcgcagg gctcggtgct gaccaacaag tatgcggaag gctatccggg
     2521 caatcgctat tacggcggct gcgaatgggt cgacgtggtg gagacgctgg cgatcgagcg
     2581 cgccaagaaa ctgttcggca cacagtttgc gaacgtgcag ccgaattccg gcagccagat
     2641 gaaccaggca gtgtttctgg cgctgctgca acccggcgat accttcatgg gtctcgatct
     2701 cgccgccggc ggacatctca cgcacggcgc gccggtgaat atttccggca aatggttcaa
     2761 ggcgcaacat tacaccgtgc gccgcgacga tcaggtcatc gacatggacg aggtcgcgcg
     2821 ccgcgccgaa gaagttaagc cgaaactgat catcgccggt gctacggcct attcacggtc
     2881 atgggatttt aaacgctttc gcgagatcgc cgacggcgtc ggggcctatc tgatggtgga
     2941 catggcgcat ttcgccggtc tcgtcgccgg tggcgtccac gcatcgccga tgccctatgc
     3001 ccacgtgacg acgacgacca cgcataagtc gctgcgcgga ccgcgcggcg gcttgatgtt
     3061 gtggaatgac gaggcgctga ccaagaagct caactcggcc gtgtttccgg gattgcaagg
     3121 cggacccttg atgcatgtga tcgcggcgaa agcggttgcg ttcgccgaag cgttgcgccc
     3181 cgacttcaag gtctacgcca agaacgtggt ggagaacgcc aaggcgctgg cggaatcgct
     3241 gcgcgccaac ggcctcgaca tcgtctccgg aggcaccgac aaccatctga tgctggttga
     3301 tctcaggccc aagggcctca agggcaacgt ctcggaaaag gcgttggtgc gcgcggcgat
     3361 cacctgcaac aagaacggca ttcccttcga ccccgagacg ccgttcgtca cgtcaggtct
     3421 tcgtctcggg acgccggcgg cgaccacgcg cggttttggg gttgccgaat tcaagcaggt
     3481 cgccggcatg atcgccgagg tgctcaatgc aatcgcgcag tcgcaggacg gcaaggcgcc
     3541 gctggtggaa agtgcgatca aggaacgggt gagggcgctc accgaccgtt tcccaatcta
     3601 tcagtaaggt ctttcccgga tgcgatgccc cagctgcaac agtctcgata cgcaggtgaa
     3661 ggactcgcgt ccaaccgagg attccgccgt gatccggcgg cggcgggttt gcgtcgcctg
     3721 caatttccgc ttcaccactt tcgagcgcgt gcagctgcgc gaactcacgg tgatcaagcg
     3781 caatggccgc cgggtgccgt tcgaccgcga caagctggtg cgctcggtgc agatctccct
     3841 gcgcaagcgg ccagtcgatc cggaacgcgt cgaaaagatg gtttcggcca tcgtgcgcga
     3901 gcttgaaagc ggcggggaag ccgagatttc ttccgaggcg atcggcgaga tcgtgatgga
     3961 gcatctgcgc cagctcgacg acgtcgccta tgtgcgcttc gcctcggtct atcgcaactt
     4021 ccgcgaagcc aaggactttg aagccgtgct cggcgagctc tccggcgacg acgacgcgcg
     4081 gattgccctg ttacgcaaat gatcttccgg attctggaag accggctcgc gcaaaaatcc
     4141 agggaatcga aggccgctga ccagcgcttc atgcagctgg cgctgacgct cggtcggcgc
     4201 gggcagggcc gcacctggcc gaacccggcc gtcggcgcgg tcgtggtcaa ggatggcgtc
     4261 attattggcc gcggctggac gcagccgggc ggccgtcccc acgccgagcc cgaggcgctc
     4321 aggcgcgccg gagaggctgc gcgcggcgcc acgctctatg tgacgctgga gccatgctcg
     4381 cacatcggaa agtcgccgcc ctgtgccgac gccatcatcg ccgccgggat cgcgcgcgtg
     4441 gtgtcggcga tcgaggatcc caatccggaa gtcgctggcc agggccatgc aaaacttcgc
     4501 gacgccggca tatcagtcga tatcgggctc ggcgccgctg aggccgcgcg cgatcacgcc
     4561 ggacacttca ggcgcgtccg cgacaagcgt ccccacgtga tcctgaaact ggcggtgtcg
     4621 tcggacgaca agatcggcgc ggccggccgc aagccggtcg caatatcggg cgacgccgca
     4681 aaagcgcggg tgcatctgtt gcgcgcgcaa tgcgacgcca tcctggtcgg gatcggcacc
     4741 gtgcgggcgg acgatccgct tctgacctgc cgcctgccgg gcatggcggc gcgctcgccg
     4801 gtgcgcgtgg tgctcgatcg taatttacgc attcccggca gctccaggct ggttcattcc
     4861 gcgcgcgaaa cgccgctgtg ggtgatgacc tcgaacctgg cggaagcacc cgccgccatg
     4921 aagctcggcg ccgccggcgc gcaggtgatc cgcgtcgcga cagcgacaac cccgccgggg
     4981 ctcgatctcg cagccgtact gcgtgcgctt tccgaaaagg gcatcaccag gctgctggtg
     5041 gaaggcggcg ctagggtagc gtcatccttt gtcgccgcag gttttgtcga tgaagtctgg
     5101 ctgttgcgcg gccctgatcc catcggcgcc gacggcgtgg ccgcgctggg cgcattgcca
     5161 ctgacgtcaa tcacgaagtc gccgaatttt cgggttcgtg ctagcaaaag cctgcaaaag
     5221 gatactctaa ccatctacga gcgcacttaa atgttcaccg gcatcattac cgacattggc
     5281 gaaatcgtca gcctgacgcc gacggcgcag gggcaattgc accggctgcg catcgcgtgc
     5341 cgctatgatc gctcgaccat tgcggatggc gcgtcgatcg cgtgcaacgg tgtctgcctc
     5401 accgtggtgg cctcaggcgt ggcgggtgac aagacctggt tcgaggtcga cgccgccgcc
     5461 gagacgctcg gcatgaccac ggcaaaacac tggacccgcg gcacaaaact caatctcgaa
     5521 cgcgcgctga agatcggcga cgaactgggc gggcatattg tcgccggaca cgccgacggc
     5581 atcgccacca tcgtcaagcg cgacgatctg cccgacatgg cgcggtttga actgcgaacc
     5641 acgcgcgagc tggcgcgctt catcgcggcc aagggctcgg tgacgctgga cggcgtgtca
     5701 ttgacggtga ataccgtgca ggacgtggta ttttcggtgc tgatcatccc gcacacgctc
     5761 aatgtgacga cgcttggcgg ttggcgcgcg ggtgccgagg tcaacctcga ggtcgacctg
     5821 atggcgcgct atgcggcgcg gcttgcggaa atgaaataga agcagccctt ggctttggac
     5881 gctgctgcgc ctaaaacgcg cgctcaatcc tcatggtgag gaggcgcttt tgcgccgtct
     5941 cgaaccatga ggccgcatcc ttcgagacgc ggccaagtgg ccgctcctca ggatgaggtc
     6001 attgcccgtt gggggctatc ggacttgaac ggacagctca atggcggacg cgcggcgcgc
     6061 acagttgaag gatcaaaccg acgtctcggg cgcccgcgcg ctgatcgtcg aggcgcgctt
     6121 ctatgacgac atccaggacg ccctgctgga aggtgcggtc gcggagctga aagccgccgg
     6181 cgtcacccac gacgtcatca cggtgcctgg cgcgttggaa attccggcgg cgatcgcgat
     6241 cgcgctcgag gccgccgaga agcgcggcag gccttacgac gccgcgattg cgctgggctg
     6301 cgtgatcagg ggcgatacca ttcatttcga aatcgtgtcg atggaatcct cgcgcgcgct
     6361 gatggatctc gctatcgcaa aacggtttcc gctcggcaac ggcatcatca ccgtcaacac
     6421 cgatgcgcag gcctgggcgc gggcgcgccc cagcgagctc aacaagggcg gcgacgccgc
     6481 ccgcgctgca ctggcgatgc tgcgcatcaa gcgccggctg atgcggacct gatcatggca
     6541 gagaacagca agaggcagga aaagaagccc gcgaagggcc cggaaaagag taaagacaag
     6601 aaggccaatc gccgcggcgc ggcacggctc gcggcggtgc aggcgcttta tcaaatggat
     6661 atcgccggcg caggcctcaa cgacatcttc gccgagttcg agagtcattg gctcggaggc
     6721 gaggtcgaag gcgacaaata ccttcccgca gaggcggcgt tcttccgtga cgtcgtctcc
     6781 ggcgtggtgc gcgaccaggc aaaactcgat ccgctgatcg atgatgcgct ttcaaaaggc
     6841 tggccgttga agcggatcga cgccatcctc cgcgcggtgc tgcgcgccgg ctcttacgag
     6901 ctcgagcacc gcaaggatgt gcccggccgc gtcgtggtgt ccgaatatgt cgatgtcgcg
     6961 catgcctttg tcgatggcga agagaccggc atggtcaacg cggtgctgga tcagatcgcc
     7021 cgccggttcc gcggcaacga gttttcgcgt gggtaggcga gatacaatga tactagccgt
     7081 agccgtcatc ctgaggtgcc gtagcgaagc ggaggcctcg aagggtgagg gcgtgtcatt
     7141 gcggcggcct ctcggccgcg catccttcga ggctcgcaag agctcgcacc tcaggatgac
     7201 gggatgacga agagcgaaca ggcgccatcc ggtgaagact cgctgatcgc gcggtatttc
     7261 aagccgctgg cgaccgatcc cggcgcgttc aatcttggcg acgatgcggc gatcctgaac
     7321 gcgtctggcg aggacaccgt ggtgacgacc gacgccatcg tcgagggcgt gcacttcctc
     7381 gcagacgatc cgcccgacac tgtcgcgcgc aaggcgctgc gggtgaacct ttccgacctt
     7441 gccgccaagg gcgcaacgcc ggcgggcttc gtgctgacgc tggcgctgcg cgccgctgac
     7501 gatgcgtggc tgacactgtt cgcgcgcggg cttggctcgg atgccggcct gttcggctgc
     7561 ccgctgctag gcggcgatac ggtttcgacg ccaggtccgc tgacgatctc gatcacggcg
     7621 ttcgggcgag tgcccgcagg cgggatggtc cgtcgcagcg gcgcgaaacc cggcgatcgc
     7681 gtcgtggtga cgggaacgat cggcgacgcc gctctcggcc tcgatatcct caagggcggc
     7741 gcggccgcgg cgcttgctga cgccggggca aaggcgatgc tgatcggacg ctatcgcgtg
     7801 ccgcagccgc gcaacgctct cgcaaaggct gttcgcgatc acgctcacgc ggcgatagat
     7861 gtgtccgatg gtctcgcggg cgatctcgcg aaactgtgcg gggcgtcggg cgtgtcggct
     7921 gtcatcgacg cgccgagcat tccattgtcc gccgccaccg cagcattgct ggcgcgcggc
     7981 accgtcggca tcgagacgat cgtttccggc ggtgacgatt acgagatctt atgcgcgatc
     8041 ccggaagata gctttgaggc attcgtgcaa gcagcaggtc aggccggagt tgccgtgact
     8101 tctataggga tggttgtcgc ggggtcgtca gttccgaggt ttttggatgg cgagggcgcg
     8161 gagttagcgc tgccgcgcct gtcctacagc catttctagg attctcgcga aaagcgccgg
     8221 tcccggcgca aatacctgcc gaaatcgaca gtttcgaccc aacgcggcgt tgcgcccgcg
     8281 gtgtgatttt ggcatggtcc cgccgattta aggccgccct tttgggcagg gcaggccttc
     8341 tttttatgcg cccgccgcgc ccgcggcgac aggcgtgggg tacaccagga tcttgaggca
     8401 agttcaatga cagcattatg ggtgatcgtg ctctgcggag cgctttcgat tctttacgcc
     8461 atctgggcga ccgcatcggt tttgaaatcg gacgccggca acccgcggat gcaggaaatc
     8521 gcggctgccg tgcgcgaagg cgcgcaggct tatctcaagc gccagtacat gacgatcggc
     8581 ctggtcggcg tcgtgatctt cgcgctcttg gcctatttcc tcggcatgct ggtcgccgtg
     8641 ggttttgcga tcggcgcggt gctgtcgggt gccgcaggct tcatcggcat gaacgtctcg
     8701 gttcgcgcca atgtgcgcac cgcgcaggct gcgaccactt cgcttgccgg ggggcttgaa
     8761 ctggccttca aggccggcgc gatcaccggc atgctggttg ccggtctcgc gctgctcggc
     8821 gtcacaatct acttcgccta tctcacccag ttccagggtc tcgccgcaaa cagccgcacc
     8881 gtggtcgatg cactggtggc gctcggcttc ggcgcgtcgc tgatttcgat ctttgcccgt
     8941 cttggcggcg gcatcttcac caagggcgct gacgtcggcg gcgatctcgt cggcaaggtc
     9001 gaggccggta ttcccgaaga cgatccgcgc aaccccgcca ccatcgccga caacgtcggc
     9061 gacaatgtcg gcgattgcgc cggcatggcc gccgacctgt tcgagaccta cgcggtgacc
     9121 acggtcgcca ccatggtatt ggccgcgatc ttctttgcga attcgccgct gctggtgaac
     9181 atgatgacgc tgccgctcgc gatcggcgcc gcctgcatca tcacctcgat catcggcacg
     9241 ttcttcgtca agctcggcgc cagccagtcc atcatgggcg cgctgtacaa gggcctgatc
     9301 gccaccggca ttctgtctct gatcggcgtt gccggcgtga tctactggct gatcggcttc
     9361 ggtccgctgg cgggcgtgaa gttcaccggc atggcgctgt tcgaatgcgg cgtcgtcggt
     9421 ctcgccgtca ccgggctgat catctggatc accgaatact acaccggcac cgaaaaccgc
     9481 ccggtgaagt cgatcgcggc ctcgtcggtc accggccacg gcaccaacgt gatccagggt
     9541 ctggcgatct cgatggaagc gaccgccgcg ccggcgctgg tgatcatcgc gggcatcctc
     9601 atcacctaca gccttgccgg cctgttcggc atcgcgatcg ccaccaccac gatgctggcg
     9661 ctggcaggga tgatcgtggc gctcgacgcg ttcggcccgg tcaccgacaa cgccggcggc
     9721 atcgccgaaa tggccggcct gcccaaggaa gtccgcaagg ccaccgacgc gcttgacgcc
     9781 gtcggcaaca ccaccaaggc cgtcaccaag ggttacgcga tcggctccgc cggtctcggc
     9841 gcgctggtgc tgtttgcggc ctacaatgaa gacctcaaat tcttcatcgc caacgcctcg
     9901 aagtttccgt actttcaggg cgtgcagccc gacttctcgc tgaacaaccc ctacgtcgtg
     9961 gtcggcctcc tgttcggcgg cctgctgccg tatttgttcg gcgcgatggg catgacggcg
    10021 gtcggccgcg cggccggcgc catcgtcgaa gaagtgcggc gtcagttccg cgagaagccc
    10081 ggcatcatgc agggcaccga caagccggac tacggcaagg ccgtcgatct tctgaccagg
    10141 gcggcgatca aggaaatgat catcccctcg ctattgccgg tgctgtcgcc gatcgtcgtt
    10201 ttcttcgcga tctattttat cgcgggcggc ggcccggccg gcaagtcggc ggcgttctcg
    10261 gccgtcggtg cgatgctgct cggcgtcatc gtcaccggtc tgttcgtcgc gatctcgatg
    10321 acctcgggcg gtggcgcctg ggacaacgcc aagaagtaca tcgaggacgg ccactacggc
    10381 ggcaagggct ccgaggccca caaggccgcg gtgaccggcg acaccgtcgg cgatccctac
    10441 aaggatacgg cgggtcccgc cgtcaacccg atgatcaaga tcaccaacat cgtggcgctg
    10501 ttgctgctgg ccatcctggc gcactgagcg gcggtatatg gcctgagaca aaccccgcgg
    10561 cgtgagccgc ggggttttca tgtccgggcc tgatcaagca tctgggatat ccgttggtcc
    10621 cactctggat gcaactgttt cagcggtgcg aggaagtgac tctcgggtcc tgccagctcg
    10681 ttcatgcggc ttgcgcagcg atgtgcatcg cccagcctgc cgagcgcgac atggcagcac
    10741 accagcgttt caagcgtcgg acggaaggtc ggacgaatgg cgagcacctt gttggcggtc
    10801 gcaagtgcgc gctcggcttc gccggcgaaa agctgtgcat aggcaagcac attgtaccaa
    10861 acgacattct gcgggtcgtt gggatttagc gccaacccat gttcaagcgg ctcgatcgcc
    10921 tcgcgcgcca ggcccgcaaa caagcgggcc atgccgagaa ccagatgtcc taacgcgaaa
    10981 cttgagctca atgcgatcgc cttctcggcc gcggatgcgg ctacgacggg tgcgttgctg
    11041 taactgttcg cgatggcgaa ggcatagtgc gcataggggt ctctcgaatc gagagttatc
    11101 gcgcgtgccg cggcatccac accctccatg ctgtccgcgc tgggagcgtc gctccagccg
    11161 taagcgatga tcccggcact aacgcgccca agccagatgt gtgattccgg caggtccgga
    11221 tcgatcgcac atgcgcggcg aaacagcgtc cgcgccgcca ggtggccctc gcgggtgagc
    11281 ttgtggaaat gccacattcc ttggcg
//
LOCUS       DATNOI010000579        10467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2564438,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000579 DATNOI010000000
VERSION     DATNOI010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10467
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2564438"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VN968_23395"
     CDS             <1..202
                     /locus_tag="VN968_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HXL32211.1"
                     /translation="PLTSSQALNNATLPFGLALANHGFAAVTQDPHLRAGLNVHRGHI
                     TNKAVADSLGLSCAPEGVIVSR"
     gene            695..2095
                     /locus_tag="VN968_23400"
     CDS             695..2095
                     /locus_tag="VN968_23400"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXL32212.1"
                     /translation="MAVIRSELRKDSLSFLEALGQSVANVSPTLTPALAVAVVAGMAG
                     TASWLVYVFATIALLIVGINVGKLAKRIPSAGSFFIYVSRSLGPSYGMLSGWAMLAAY
                     IFTAMALTVATSIFIKTFFTSLGFTAPIPSTLLYFLISAGIWAFACRDIKISARIGLT
                     LEVVSVAIILFVCVYTFAHFGFKIDAKQTHLEGIDFKSAAQAVVFAIFSYVGFESAAT
                     LGRETRNPTVNIPRAIVATAISAGVFFVLTTYLIVQGFDDDATKLGASGAPLADITSS
                     ISVGLTALVYFGAAISGFACALASLNAYGRMLFSLGRYQFVHTSMGLVHQNFKTPYVA
                     LTSGALINFIVCAAFAGQAETDTFGWYGTLASYGFIIVYFLCSVSAPVFLAKNGGATG
                     GDKFVGGLGAVLMVLSLVGSLYPVPASPYNLFPYFFLVYILLGVSWFFILKARVPQAI
                     LGIEHDLEIAASTPAE"
     gene            2331..4040
                     /locus_tag="VN968_23405"
     CDS             2331..4040
                     /locus_tag="VN968_23405"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXL32213.1"
                     /translation="MSQQPSTLNASGWTLAVGASSSASATSEGADVYVIHKGEAFDRP
                     GRGIIAAVARGIGAGPRAREAAHIALHGLLEGYFGAAATLGPGRAAGLALGSANAWMF
                     GRSRADPGRPMAASLTALIFVGRRVRIVHVGDGRVYRRRGSQLTPLTSDHVHPAADGT
                     GLLTRSVGGDAELQMDYLEDACEPADRYFLLSKGGFASTPSTRLADVLTATWTADEAA
                     GLIVASIPDKAGASAATAIVIDVLKAPDSSFDEMAASFLRLPIRGAPRDGENWDGFLL
                     RRTLYRSRYTTLKLARDTIGNKDVVLKIPLPSMLHDEVFRAGFLREAWVGATAHSPRI
                     ASYIEVAPERRSSLYLVLPYYRGETLEARLSRMDPIGYLEGVGLALRLCAAVQDLAAL
                     QIVHRDLKPENILLLPDGEIKLLDLGLAYLPGVDEPDDDRLGGTTRYMAPELFRKQPA
                     DQRSEVFSLGVTVYRLFSGGPFPFGQRETIPLQRHRPDLPAWLGQCLIDAMEKNPEKR
                     FKDASEFASALENGLSQGAMRPLGTLPPQHRWVDPLRLWQGLALLFAVAFFVLLIMLL
                     HPR"
     gene            complement(4091..4303)
                     /locus_tag="VN968_23410"
     CDS             complement(4091..4303)
                     /locus_tag="VN968_23410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32214.1"
                     /translation="MSRFVVRFLKDVLGENGREAEICQGSLEIDASDKVDATETAKRK
                     FCEAQALRHWSLHADRIQVTEADFPS"
     gene            complement(4469..5473)
                     /locus_tag="VN968_23415"
     CDS             complement(4469..5473)
                     /locus_tag="VN968_23415"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL32215.1"
                     /translation="MRSACTWTLAVAGRSVTALALVFSLSALVTGSARADDYPNRPVK
                     IIVPFAAGGTADAIPRLVGDWLSRKWGKPVVIENRTGAAGNIGADFVYHSDPDGYTLL
                     SSPPPPLVINQNLYPKLGFDPAKFEPVIVMAQVPNALIVNPKNLTASSVSELIDYLQQ
                     NPEKVTAATQGIGTTSHLTAELFQVMAKVKLRTIPYRGSAPALAGLLAGDVDLMFDNL
                     GVSLPLVEAGNLKLLAVASGQRLPSLPDVPTIAETLPGFEAVAWFAVTAPPKTPKNIT
                     DKINADVNEALHQPELQDRLRKLSAETFGGSVDKMAKYMREEVERWGNVIKAADIKLQ
                     "
     gene            5597..6778
                     /locus_tag="VN968_23420"
     CDS             5597..6778
                     /locus_tag="VN968_23420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXL32216.1"
                     /translation="MISRPHVAIVGGGIGGLFAANALAAQGIRVSVYEQAPAIGEIGA
                     GVFLTPNSVRHLQRIGLEPAVEQWGARVGPDSRYFRHDGAPIAPVQVTDSSGWNATFG
                     MHRADLVAMLAEALPAGTVHTGHRCCGFEQDGNTACVSFANGVSVEADVVIAADGIHS
                     ELRPYVVPSSRPVFSGSVAYRGLVPHERVPHWPTDRWQMWLGKGRHFLAFPVRAGKLI
                     NYVGFMPTDEAMKESWSAPGDPNVLREAFAGWDPRINELLTQVQVTFRWALYDREPLP
                     VWTRERLSLLGDAAHPMLPHLGQGANQSIEDGMALATILKDADRNTAPKALRAYERLR
                     RERVAEVQRGARENGMRYDSAYSDLGVRDAEITAHAAFRKRLYDHDVFPDAQAAAATL
                     I"
     gene            6884..7738
                     /locus_tag="VN968_23425"
     CDS             6884..7738
                     /locus_tag="VN968_23425"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL32217.1"
                     /translation="MTRISIVTGATRNLGFSLAQGLARRLEQDDVVYLTGRDTGRVAE
                     SVRLLSGHRANVTGEVLDVSDRDAVERFANALADRHGGVDIVFSNHYARVQPGENAAE
                     IIDGYVAANNLGTTHILRSFAPLVRDNGRLLVVASRAGSLRALAPALHGRFENLQTLD
                     DVDRAVCAWRDVVRQGLAAGQAWPEWINIPSKIGQVAAVRVLAEQRRAEDMRRRILIA
                     AISPGLIDTGASRQWLDMTGAPTPDEVAIPLLDLALEEAPDTAFYGGLIHLGHGEPGW
                     FRTLVPWI"
     gene            7695..8195
                     /locus_tag="VN968_23430"
     CDS             7695..8195
                     /locus_tag="VN968_23430"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXL32218.1"
                     /translation="MVSLGGFGHWCPGYETPEMKQRKKAGADERSVGMLLKRAEQAML
                     KSKTAALKPVGLTLAQYVALAELEQKQGIRAATLARACFVSPQAIMILLKSMEQQGLI
                     TRRPHPRHPNVLELHMTDVGREALHAGRAQVAPVEESIFAAFSKQELEAFRGFLSRFV
                     SAIDAG"
     gene            complement(8642..9691)
                     /locus_tag="VN968_23435"
     CDS             complement(8642..9691)
                     /locus_tag="VN968_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HXL32219.1"
                     /translation="MSVNNKRVFYVKYLAHEIYADILRARPDVRLDRLENESPDEISA
                     PILAAAHAYQIGAARDELAPRFHVDREVLRRAPNLLIVSSNGAGYDPVDVEACTGAGV
                     VVVNQSGGNAHSVAEHALAMLLTLSKRIIEADRALRRDPNVNRNALIGTEAGGKTIGI
                     VGLGNVGRRIAALCKGLLGMNVLAYDPYLSTTEMAERGGAKVELAELLRRSDYVSISC
                     PLTKESRGMIGAREFAAMQPHAYFITTARGFIHDEAALLEALRNKSIAGAGLDVWTKE
                     PPPPDHPLLQFDNVLASPHTAGVTREARKNMGRIAAEQMLDVLDGKRPPRIINPEVWP
                     DYAKRFERTFGFAPG"
     gene            complement(9822..>10467)
                     /locus_tag="VN968_23440"
     CDS             complement(9822..>10467)
                     /locus_tag="VN968_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL32220.1"
                     /translation="YDTFKDFAPISLLASSPNILLVRADSPFKTLGDALAQARAKPGS
                     LSYGHAGTGTSTHLAGELLKNLGRIDLNAIPYKGGAPSINDLLGGQIPMSFNNGPESV
                     GQLEAGTVRALAVTTASRAPFLPDVPSMAETVPGYDTEVWWGLLGPAGMAPDLVAKLS
                     HDFVAALNTEPVKERLAKLGAAPIGSSPEQFDARIRADYKKWGPIIEAAGMKAE"
BASE COUNT         1871 a         3259 c         3304 g         2033 t
ORIGIN      
        1 tccactgacg tcgagccagg ccttgaacaa tgcgacgctg ccattcgggc tggctttagc
       61 gaaccatggc tttgcagccg tgacgcagga tccccatctc cgggcgggcc tgaatgttca
      121 ccgcggccat atcaccaaca aagcggtggc cgacagtctt ggcctgtcgt gcgcgcccga
      181 gggcgtgatc gtttcgaggt agcggcacgc gcaattgatc tcggcgctgc gaatgcggcc
      241 gtccaggccg atcgcgcgcg gtcgcagtga tgctgtggcc gcttgccgac ttacgcgatc
      301 tcggcttttc cgaagcttga cccgccgcgc ttgcatcggc cgtcagtgac acgccaaaaa
      361 attcgccttc gccgaggccc gcgctgaagc accggatcgg tcgggcacga cgtcgcgtgg
      421 ttcgacagca gccgcacaaa tcttagacac agtggacagg cgatgggctc cggccacgcg
      481 ccccgcggcc aagaacaatt ctttcctgaa atgaaaagca agtgggcgtc ccgaaaagcc
      541 cggcaaacca gcaattttcc gctgcggcac gcatcttgct aagaaactat tttgcatgtc
      601 aggaaatcgg ctcggcctca caacccagcg ggcggaacca tggatggcga tcggcgggca
      661 gccggaagac catcccaaaa acgggagacg gatgatggcg gtgatacgat cggaactgcg
      721 gaaggatagt ttgtcgttct tggaagcgct cggtcagtcg gttgcgaacg tttcgccgac
      781 gctgacgccg gcactggcgg tcgcggtggt ggcgggcatg gccggcaccg catcgtggct
      841 ggtctacgtc ttcgcgacca tcgcgctgtt gatcgtcggc atcaatgtgg gcaagctcgc
      901 caagcgcatc ccctctgccg gctcgttctt catctatgtc tcacgcagtc tcggtccatc
      961 ctacggcatg ttgtccggct gggcgatgct ggcggcctat atcttcaccg cgatggcgct
     1021 gacggttgcc acctccatct tcatcaagac gttcttcact tcgctcggct tcaccgcccc
     1081 tattcccagc acgctgctgt atttcctgat ctcggccggg atctgggcct tcgcctgccg
     1141 tgacatcaag atttcggcgc gcatcgggct gacgcttgag gtggtatcgg tcgccatcat
     1201 cctgttcgtg tgcgtctata cgtttgccca tttcggcttc aagatcgatg ccaagcaaac
     1261 tcatctcgag ggcattgact tcaagagcgc cgcgcaggct gtcgtgttcg ccatcttctc
     1321 ctatgtcggc ttcgagagcg cggcgacgct gggccgggaa acccgcaatc cgaccgtcaa
     1381 catacccagg gccatcgtcg ccacggcgat ctccgccggc gtgttcttcg tgctgacgac
     1441 ctatctgatc gtgcagggct ttgacgacga tgccaccaag cttggtgcca gtggcgcgcc
     1501 gctcgccgac atcaccagca gcatcagcgt aggcttgacc gcgctggtgt atttcggcgc
     1561 cgcgatcagc ggcttcgcct gcgcgctggc gtcgctgaat gcctacggac gaatgctgtt
     1621 ctcgctcgga cgctaccagt tcgttcacac ctcgatggga ctggtgcatc agaacttcaa
     1681 gacaccctat gtggccttga cctcaggtgc gctgatcaac ttcatcgtct gcgccgcgtt
     1741 tgccggtcag gcggaaaccg acacctttgg ctggtacggc accctcgcct cctacggttt
     1801 catcatcgtg tacttcctgt gctcggtaag cgcgccggtg tttttggcga aaaacggcgg
     1861 cgccacgggc ggcgacaaat tcgtcggggg tctcggcgcg gtactgatgg tgctgtcgtt
     1921 ggtcggcagc ctctatccgg tgccggcctc gccatacaat ctgtttccct acttcttcct
     1981 ggtctacatc ctcttggggg tgagctggtt cttcattctg aaggcgcgcg tcccgcaggc
     2041 gatcctcggc atcgagcatg atctggaaat agcggcatcg acgcccgccg agtagtcgag
     2101 gccgaataga gtcagacggc cgcgaccgct tcgaagcgcc gccgctgtga tgacccaggg
     2161 gttgcttccc cggcgctacg acccctgcgt ctgccacacc ccttccgctt cggaaggggt
     2221 gtgtgcttgc cagccgttca cgctcccaag atactggtcg tcggatccga tgcgtaagcc
     2281 gggcggctga ctggtcatgt gcaggctggc gcccctccaa ggccagcgaa atgagccagc
     2341 agccatcaac gctcaatgcc tcgggatgga cgctggcggt cggagcatct tcgtcggctt
     2401 ccgcaacgtc cgagggcgcg gatgtatatg tcattcataa aggcgaagcc ttcgaccgac
     2461 ccggccgtgg catcatcgcc gccgtcgcgc gcggcatcgg cgccgggccc cgcgcgcgcg
     2521 aagcggcaca tatcgcgctg cacggcctgc tggaaggtta ctttggcgcg gcggccacgc
     2581 ttggcccggg gcgtgccgcc ggtcttgcgt tgggaagtgc caacgcctgg atgttcggcc
     2641 ggtcccgcgc cgatcccggg cgccccatgg cggcttcgct gacggcgctc atctttgtcg
     2701 gccgacgcgt ccggatcgtc catgtcggcg acggtcgcgt ttatcgccga cgcggcagcc
     2761 aacttacgcc gctgacgtcg gatcatgtgc acccggccgc cgacggaacc ggcttgctga
     2821 cgcggtcggt cggcggcgat gccgagttgc agatggatta tctcgaagac gcctgcgaac
     2881 cggccgaccg ctatttctta ttgagcaaag gcggctttgc ctcaacaccg tcgacgcggc
     2941 ttgccgatgt gctcacggcg acgtggacag ccgacgaagc cgccggcctg atcgtcgcgt
     3001 cgatccccga caaggccggg gccagcgccg cgaccgcgat cgtgatcgac gttctcaagg
     3061 cacccgactc gagcttcgat gaaatggcag cgtcattcct gcggctgccg atacgaggcg
     3121 cgccgcgcga cggcgagaat tgggatggat ttttgcttcg ccgcacgctc tatcgcagcc
     3181 gttacaccac cctgaaactg gctcgcgaca ccatcggcaa caaggacgtt gttctcaaga
     3241 tacccctgcc ctcgatgttg cacgacgagg tctttcgggc cggattcctg cgcgaggcct
     3301 gggtcggcgc caccgctcac agcccgcgaa ttgccagcta tatcgaggtc gccccggaac
     3361 gccgcagcag cctgtatctc gtgctcccgt attatcgcgg agagacgctt gaggcccgcc
     3421 tgtcgaggat ggacccaatc ggctatctcg aaggcgttgg gcttgcgcta agactttgcg
     3481 cggcggtgca ggatctggcg gcattgcaga tcgttcaccg cgacctcaag ccggagaaca
     3541 ttcttctgct tcccgatggc gagatcaaac tgctcgacct cggcctcgcc tacctgcccg
     3601 gcgtcgacga gcccgacgac gatcgtctgg gcggcaccac ccgctacatg gcgcccgagc
     3661 tgttccgaaa acagccggcg gatcaacgct ccgaggtatt ttcgctggga gtgacggtct
     3721 atcgcctgtt ctccggtggc ccgtttccgt tcgggcagcg cgaaaccatc ccgctgcaaa
     3781 ggcaccgtcc cgacctgccg gcctggctcg gccagtgcct gatcgacgcg atggaaaaga
     3841 atcccgaaaa gcgtttcaag gacgcaagcg agtttgccag cgcgctggaa aatggcttga
     3901 gccagggcgc gatgaggccg ctcggcacct tgccgccgca gcatcgatgg gtcgatccgt
     3961 tgcggctttg gcagggactc gccttgctgt ttgccgtggc atttttcgtc ctgctgatca
     4021 tgttgctgca cccgcgatga caggcatgca gctgaagcac cagaatgcgg gtgcgtcggc
     4081 atcaagacct tcaggacgga aaatcggcct cggtgacctg gattctgtcg gcgtgaagcg
     4141 accagtggcg aagcgcctgc gcttcgcaga attttcgctt tgccgtttcc gtggcatcga
     4201 ccttgtcgga agcgtcaatt tccagcgaac cctgacaaat ttccgcctcc cggccgtttt
     4261 cgccgagcac atccttcaag aagcgaacga caaatcgtga catcaaagcc tcctgttgcg
     4321 gcggcagcac caaaaccagt tccatgtaat ctgtgggtgc ggaaatcgtt ttgacaggga
     4381 tcaaaccgcg ccggattgac ggcaccgcaa ttcctggcga gccaaaatcg atcaatgccg
     4441 tcccaaaagt cggccttttg cccggcgatt attgaagctt gatgtccgct gccttgatca
     4501 cattacccca ccgctcgacc tcctcccgca tgtacttcgc catcttgtcc acggagccgc
     4561 caaacgtctc cgccgacagt ttcctgagac ggtcctgcaa ctcgggttga tgcagggctt
     4621 cgttcacatc ggcattgatc ttgtcggtga tgtttttcgg cgtcttcggt ggcgccgtga
     4681 ccgcgaacca ggcgacggcc tcgaagccgg gcaacgtttc ggcaatggtc ggcacgtctg
     4741 gaagcgacgg caggcgctgg cccgatgcca cggcgagcag cttgagattg ccggcctcga
     4801 cgaggggcaa ggaaacaccg agattgtcga acatcaggtc gacgtcgccg gcaagcaagc
     4861 cggcaagtgc tggcgccgac cctcgatacg ggatggtgcg cagtttcacc ttggccatca
     4921 cctggaaaag ttcggcggtc agatgcgagg tcgtgccgat gccctgcgtg gcggccgtga
     4981 ccttctcggg gttctgttgc aggtaatcga tgagctcgga cacgctcgac gccgtgaggt
     5041 tttttggatt gacgatcagc gcgttcggaa cctgggccat cacgatgacg ggctcgaatt
     5101 tcgccggatc aaatccgagc ttcggataga gattctggtt gatcacaagc ggcggcggcg
     5161 gtgacgaaag caacgtgtag ccgtcggggt ccgaatgata gacaaaatca gcaccgatgt
     5221 ttccagcggc cccggtgcgg ttctcgataa cgaccggctt gccccatttg cgcgagagcc
     5281 aatccccgac cagccgcggg atggcatcgg ccgtcccgcc ggcggcgaac ggcacaatga
     5341 tttttacggg acggtttgga tagtcgtcgg cacgcgccga accggtgacg agcgccgaaa
     5401 gagagaacac cagcgccagc gcggtcacgg atcggccggc aacagccaat gtccaagtgc
     5461 atgcgctacg catcagatgc ccttccccaa tatcccggcg ggtctgaccg cgccagatgg
     5521 caaaatgcct tggtctgtgt gaggatacaa tgccgtagcc aattttggtc gcgcattccg
     5581 tgacaaggag ttgatcatga tttcccgtcc ccatgtcgcg atcgttggtg gcgggatcgg
     5641 ggggttattc gcggccaatg cgctggccgc gcaaggtatc cgggtttcgg tctacgagca
     5701 ggcgcctgcg atcggcgaga tcggggccgg cgtgtttctc acccccaaca gcgtacgcca
     5761 tctgcaaaga ataggattgg agccggcggt cgagcaatgg ggagcgcgtg tcggcccgga
     5821 ttcccgctac ttccgccacg atggcgcgcc gatcgcgccg gtccaggtga cggattcctc
     5881 gggctggaac gcgacgttcg ggatgcatcg cgccgacctc gtggcgatgt tggccgaagc
     5941 gctgccggcc ggaacggtgc ataccggaca tcggtgctgc gggttcgagc aggacggcaa
     6001 cacagcctgc gtgtcgttcg ccaatggcgt ctcggtcgaa gccgatgttg tcatcgccgc
     6061 cgacggcatc cattccgaac ttcggcctta tgttgttccg tcgtcgcggc cggtgttctc
     6121 gggatcggtc gcgtaccgcg gtctggtgcc gcatgagcgc gttccgcatt ggccgaccga
     6181 ccgctggcag atgtggctcg gcaaaggaag acattttctt gcctttcccg tccgcgccgg
     6241 aaagctgatc aactatgtcg ggttcatgcc gacggacgag gcgatgaagg aatcctggtc
     6301 ggcccccgga gaccccaacg ttctacggga ggcgtttgcc gggtgggacc cgcgcatcaa
     6361 tgaactattg acgcaagttc aggtgacctt ccgatgggcg ctgtatgatc gcgagccact
     6421 gccggtctgg acaagggaaa ggctgagcct gctcggcgac gccgcgcatc cgatgctgcc
     6481 gcatctcgga caaggcgcca atcaatcgat cgaagatggc atggcgctcg cgacgatcct
     6541 gaaggatgca gaccgaaaca cggctccaaa ggccctgcgc gcctacgaac ggctccgccg
     6601 cgagcgcgtg gccgaggtgc agcgcggcgc gcgcgagaac gggatgcggt acgactccgc
     6661 ctactccgac ctcggtgtca gggacgccga gatcaccgcg cacgccgcat tccgcaagcg
     6721 gctctacgac cacgacgtgt tcccggacgc gcaggccgcg gcggcaacgc tgatttagct
     6781 ggttgagtgt tgcaggactg gtgatgtgcc ggcccgcgtc agatctggtt cctgattctt
     6841 atcaacatat tgacaacaac aatatcattg atatacttcc cgcatgacaa ggatttcgat
     6901 cgtgacgggc gcgacccgca atctcggctt cagcctcgcc caggggcttg ctcgccggct
     6961 ggaacaggac gacgtggttt acctgacggg gcgggacacg ggccgcgtcg ccgaatcggt
     7021 ccgtcttctg tccggtcatc gagcaaacgt aacaggggaa gttctcgacg tgtcggatcg
     7081 tgacgcggtg gagcgctttg caaatgctct cgccgatcgc catggtggcg tcgatatcgt
     7141 attctcgaac cactacgcac gggtgcagcc gggcgagaat gccgctgaaa ttatcgacgg
     7201 ctatgttgcc gccaacaacc tcgggacaac tcatatcttg cggagttttg cgccgcttgt
     7261 tcgcgataac gggcggctat tggtggtggc gagccgcgca ggcagcttgc gcgccttggc
     7321 ccccgcgctg cacgggcgtt tcgagaatct tcaaaccctt gatgacgttg accgcgcggt
     7381 gtgcgcgtgg cgcgatgtcg ttcgtcaggg acttgcggcc ggacaggcct ggccggagtg
     7441 gatcaacatt ccctcgaaga tcggccaagt cgccgccgtt cgcgtgctgg cggagcaacg
     7501 gcgggcggag gatatgcggc ggagaatcct gatcgccgcg atctcgcccg ggctgatcga
     7561 caccggcgcg tcgcggcaat ggctcgatat gaccggggcg ccaacgcctg atgaagttgc
     7621 gatcccgctg ttggatcttg cgttggagga agcgccggac acggccttct acggcgggtt
     7681 gatccacctc ggtcatggtg agcctgggtg gtttcggaca ctggtgccct ggatatgaaa
     7741 ccccggagat gaaacaacgc aagaaagctg gagcggacga gcgaagcgtc ggcatgcttc
     7801 tcaagcgtgc tgaacaggcg atgctcaagt ccaaaaccgc ggcgctcaag ccagtgggac
     7861 ttacgcttgc gcaatatgtt gcgctggcgg aactcgaaca gaaacagggc atcagggcgg
     7921 cgacgctggc gcgggcctgc tttgtgagtc ctcaggccat catgattctg cttaaaagca
     7981 tggagcagca ggggctaatc acgcggcggc ctcatccaag gcatccgaat gtgctcgaac
     8041 ttcacatgac cgatgtcggc cgagaggcgt tgcacgccgg tcgcgcccaa gtcgctccgg
     8101 tggaggagag catcttcgcg gccttctcca agcaggagct ggaggccttc cgcggtttcc
     8161 tttcccgctt cgtcagcgcg atcgatgcgg gatagagatg cggggacagg ctcgccagcc
     8221 ttggtggagt tgaagacgta attttccgta cgccgcgggc tcgcgggcgg acctgagcga
     8281 taggcaatgg tcgtgtgaaa acgcctaggc gccggcggaa ggcaggctcg ccgccgcgat
     8341 ggcgacgatc agcgccagac ggcgtccgat ccgggacggc gccgagcgca acaatgaacg
     8401 cagatgtcgc atatcgtaga ttccctctgc gccacccgaa acaatcgttt cgcaaagcag
     8461 agcgcagaac gagaacatgg ttccgggctc tacgggtgtc gagaacgcgg cgaaaatagc
     8521 aacgcgtcgt tcgtccggcc gccgtttgac agtggattta ttgccgccat ccagccgccg
     8581 acggtcgaaa ttttctctct cgaaaatccc ccgcgccgcc cgatgcgccg gtccggaacc
     8641 cctaccccgg cgcaaatccg aaagtccgct cgaaacgttt ggcgtagtcc ggccagacct
     8701 cgggattgat gatgcgcggc ggccgcttgc cgtcgagcac atcgagcatt tgctcggcgg
     8761 cgatccggcc catatttttc cgcgcttccc tggtcactcc ggccgtatgc gggctcgcca
     8821 gcacattatc gaactgcagc agcggatggt ccggcggcgg cggttccttg gtccagacgt
     8881 cgagacccgc tccggcgatc gatttgttgc gcagtgcttc aagcagtgct gcttcatcat
     8941 ggataaagcc ccgcgccgtg gtgatgaaat aggcatgcgg ctgcatagct gcgaattcgc
     9001 gcgcgccgat catgccgcgg ctctctttgg tcagcggaca ggagatcgag acgtagtccg
     9061 agcggcgcag cagttcggcc agttcgacct tggcgccacc ccgctccgcc atctcggttg
     9121 tcgagagata gggatcgtag gccagcacgt tcatgccgag caggcccttg caaagcgcgg
     9181 cgatgcggcg gccgacattg cctaacccga cgatgccgat ggtcttgccc cctgcctcgg
     9241 tgccgatcaa ggcgttgcga ttgacgttgg ggtcgcgccg cagcgcacgg tcggcctcga
     9301 tgatgcgctt tgataaagtc aggagcatcg ccagcgcgtg ttcggccacc gaatgggcat
     9361 tgccgccgga ctggttgacg acgacgacgc cggcccccgt gcaggcctcg acatcgaccg
     9421 gatcgtagcc cgcgccattg ctggacacga tcaagaggtt cggcgcgcgc cgcagcacct
     9481 cgcggtcgac gtggaagcgg ggtgcgagtt cgtcgcgcgc tgcaccgatt tgataggcat
     9541 gcgccgcggc caggatcggc gccgagatct cgtcaggact ttcattctcg agccggtcca
     9601 gccgcacatc gggccgcgcc cgcaggatgt cggcatagat ctcatgcgcc agatacttga
     9661 cgtaaaacac acgcttgttg ttgacggaca ttctattgct agccttcagt tcggaaatcc
     9721 ataaagcctt gcgagattgg tcacgaggat gcgctgctgc gtcgacgggg ccggccagtc
     9781 gccgccccat accggtgctt ccggattggc ccgcaccaga ctcattcggc cttcatgccg
     9841 gcagcctcga tgatcggccc ccacttcttg tagtcggcgc gaatcctggc gtcgaattgc
     9901 tccggcgaac tgccgatcgg cgccgcgccg agcttggcga gacgctcctt caccggctcc
     9961 gtgttcaagg ccgcgacaaa atcatgcgag agtttcgcga cgagatcggg ggccatgccg
    10021 gccggtccga gcaagcccca ccacacttcg gtgtcgtagc cgggcacggt ctcggccatg
    10081 ctgggcacat cgggcagaaa cggcgcgcgc gatgcggtcg tgacagcgag cgcgcggacg
    10141 gtgccggctt cgagctggcc gacggattcc gggccgttgt tgaaggacat cgggatctgc
    10201 ccgcccagga ggtcgttgat cgagggcgcg ccgcccttgt agggaattgc gttgagatcg
    10261 atcctgccaa gattcttcag aagctcgccg gccagatgcg tcgatgttcc cgtccccgca
    10321 tggccgtagg acaggcttcc cggcttggcg cgcgcctgcg cgagcgcatc gcccagcgtc
    10381 ttgaacggag aatcggcgcg caccagcagg atgttgggag acgaggccaa tagcgagatc
    10441 ggcgcaaaat ccttgaaggt gtcgtag
//
LOCUS       DATNOI010000580         2051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_801794,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000580 DATNOI010000000
VERSION     DATNOI010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801794"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VN968_23445"
     CDS             <1..1006
                     /locus_tag="VN968_23445"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXL32221.1"
                     /translation="DRVGALLGAAPGQTLVCDTTSINLYKAVHAAIGLRPGRDVVIAE
                     DESFPTDLYIVEGAMKSAGRPMKRRLIGTDGPSIDALLDETVAVAVLSHVNYRTGALL
                     DMATITKQLHDAGALVVWDLCHSIGVIEIAFDLHAVDFAVGCTYKYLNGGPGSPAFVS
                     VAKAHQAAARHPLSGWWGHAAPFAFDRDFRPDEGIKRFLCGTQPIISLRGVDVALDAM
                     EGVEVADLRQKSLALTELFMARVTALLPGLDIVTPRQPSLRGSQVAISFDKGYAVVQA
                     MIEHGVIGDFRAPDIMRFGFAPLYIRFQDVWDAADILAGCINAEVWRDPRFNRYLDVT
                     "
     gene            complement(1032..1166)
                     /locus_tag="VN968_23450"
     CDS             complement(1032..1166)
                     /locus_tag="VN968_23450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32222.1"
                     /translation="MTLFAKIFTTKTLLQIILVLIATVMMKAISAHYDFSWATAFAQG
                     "
     gene            complement(1281..>2051)
                     /locus_tag="VN968_23455"
     CDS             complement(1281..>2051)
                     /locus_tag="VN968_23455"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXL32223.1"
                     /translation="PGGKYTAKDVYDIGGTAVVVRALIESGHIDGSCLTITGRTVAEE
                     YGGANAPDGEIITSVGAAISPDGGVAVLKGNLCPDGAVIKVAGLKKLVFEGTARVFED
                     EEECVDAVRNRKYAAGDVLVIRNEGPVGGPGMREMLGVTALIYGQGMGEKVALITDGR
                     FSGATRGMCIGYVSPESFIGGPLALVQEGDRIRIDAGARRLDLLINDNEFAKRRQAWK
                     QRPPRHRAGALAKYARLVGQAPGGAVTHDGAAEWPWFD"
BASE COUNT          377 a          659 c          603 g          412 t
ORIGIN      
        1 cgaccgcgtc ggggcactgc ttggggcggc tccgggtcaa accctggttt gcgacaccac
       61 atcgatcaat ctctacaagg cagtccatgc cgcaatcgga ttgaggcccg gccgcgacgt
      121 ggtcatcgcc gaggatgagt cgttcccgac agacctctat atcgtcgaag gcgcaatgaa
      181 gtccgcgggt cgcccgatga agcgccgatt gatcggtacg gatggcccct ccatcgacgc
      241 cctgctcgac gaaacggtcg cggtcgccgt gctctcgcat gttaattatc gcacaggcgc
      301 gctgctcgac atggccacca tcaccaaaca acttcacgac gccggtgcgc tggtggtgtg
      361 ggacctctgt cattcgatag gcgtgatcga aatcgcgttc gaccttcatg cggtcgattt
      421 tgcagtcggc tgcacctaca aatacctgaa cggaggtccc ggatctccag cctttgtttc
      481 cgtcgcaaaa gcccatcagg ccgccgcaag acatccgctg tcaggctggt ggggtcatgc
      541 tgccccgttt gcgttcgatc gtgacttccg gcccgacgaa ggtatcaaac gctttctctg
      601 cggaacccag ccgatcattt cgctgcgcgg cgtcgatgtg gctctcgacg cgatggaggg
      661 cgtggaggtg gccgatttga gacagaagag cctcgcgctc acggagcttt tcatggcacg
      721 cgttaccgcg cttttgccgg gccttgatat cgtgacaccc cgccaaccat cattgcgcgg
      781 cagccaggtc gcgatatcct tcgacaaggg ttatgcggtc gtgcaggcga tgatcgagca
      841 cggtgtgatc ggcgactttc gggcgcccga catcatgcgc tttggcttcg cgccgcttta
      901 cattcgcttt caggatgtct gggatgcggc cgatatactc gccgggtgca taaacgcaga
      961 ggtctggcgc gatccccgct tcaatcgtta tctggatgtc acctgagcgg tgcgaagccg
     1021 ccggtgaaaa gttacccctg cgcgaacgcg gtggcccagc tgaaatcata atgggcgctg
     1081 atcgccttca tcatgaccgt agcgatcaaa acgagaatga tctgtaacag cgttttcgtc
     1141 gtgaatatct ttgcaaacag cgtcatgtga tgctcccagc aggcgtatac ggcaccgagg
     1201 cccgctaatc aagaacggcg caacggtttc cggcgcagga aatgaggtgg gacaattcga
     1261 acgactgggc cacctcaacg ctagtcgaac catggccatt ccgcggcgcc gtcgtgggtg
     1321 acggcgccgc cgggcgcctg gccgaccagc cgcgcgtact tggcgagcgc ccctgcccgg
     1381 tgccgcggcg gccgctgctt ccaggcctgc cgtcgctttg cgaattcatt gtcgttgatc
     1441 agtagatcca ggcggcgcgc acccgcatcg atgcgaatcc gatccccctc ctgcaccagc
     1501 gccagcgggc cgccgataaa cgattccggc gagacgtaac cgatgcacat cccccgggtg
     1561 gcgccggaaa agcgcccgtc ggtgataagt gcgaccttct ctcccattcc ctgtccgtaa
     1621 atcagcgcgg tgacccccag catctcgcgc atgccgggac cgcccacggg gccttcatta
     1681 cggatgacga gaacgtcgcc tgccgcgtat ttgcggttgc ggacggcatc gacgcattcc
     1741 tcctcatctt cgaacacgcg cgcggtacct tcgaacacca gttttttgag gccagccacc
     1801 ttgatgacgg ctccgtcagg gcaaaggttg cccttcaaaa ccgcgacgcc accgtccgga
     1861 ctgatcgccg cgcctacaga ggtgatgatc tcgccgtcgg gcgcattggc cccgccgtat
     1921 tcttcggcaa ccgtccggcc ggtaatcgtc aggcaactgc cgtcgatgtg accgctttcg
     1981 atcagcgcgc ggaccaccac ggcagtgccg ccgatatcgt agacatcctt cgcggtatat
     2041 tttccgcccg g
//
LOCUS       DATNOI010000581         9633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_425456,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000581 DATNOI010000000
VERSION     DATNOI010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9633
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425456"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VN968_23460"
     CDS             <1..435
                     /locus_tag="VN968_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HXL32224.1"
                     /translation="PVGTGVWPAFWMLANDGGWPPELDVMEGRGQQPGDLVMTTHWRN
                     RTTGLVQSCGFDFSRPDASSTFHDYGVLWAQDQVVYFIDRKPAADIKTPVGFDDPMYM
                     IVNLAMGAKYFKGVGFVDGMSPDTVAFEIDRISAYQIDPYSK"
     gene            454..1926
                     /locus_tag="VN968_23465"
     CDS             454..1926
                     /locus_tag="VN968_23465"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HXL32225.1"
                     /translation="MFGKFSRRHFVKLAGLSAFGMAATPTKPAESETKPAADGHAPAA
                     FPDGFLWGTATSAYQIEGAVDEDGRGRSIWDTFSHTPGKIEDHSNADRANDHYHRYKE
                     DVGLIKELGVKAYRFSIAWPRVFPQGTGAPNPKGLDFYDRLLDELLKDGIEPFATLYH
                     WDLPQALQDRVGGWQSSDTSRAFADYAGYVAERLTDRVKNIFTLNEAGRFLNFGYGWG
                     IDAPGLKLPPAELNQARHHVVLAHGLAVQAIRAHGRTGTKVGPAENIAACVPAIDTPE
                     NVRAAEIATRELNAGFLGVILEGKYTDGFLQYAGANAPKFTADELKIISSPNDFVGLN
                     IYAPQFYVVAADRAPGFKVLPFPASFPHMNSEWLRVGPETIYWVPRLAAKIWSIDTIY
                     ISENGTSSADDLAADGNVYDLDRIMYLRNYLTQLQRATSEGVPVRGYFLWSLMDNFEW
                     IFGFEKRFGLYRVDFETQRRVAKLSASFYRDVVARNAIGT"
     gene            complement(1959..3575)
                     /locus_tag="VN968_23470"
     CDS             complement(1959..3575)
                     /locus_tag="VN968_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HXL32226.1"
                     /translation="MSAGAETAASSGWSPERSAPGGHNPYLIAFVVSIATFMEVLDTT
                     IANVALRHIAGGLAVGIDESTYVITSYLVANAIVLSISGWLSTVIGRKQFYMICVATF
                     AVASLLCGLAWSLQSLVFFRIIQGLGGGGMATSEQAILADSFPPAKRGQAFAIYGVAV
                     VVAPVVGPTLGGWITDTYSWHWVFLINVPMGLISLFLVGTLVSEPSGAEEERARLLSG
                     GVRIDYVGFLLIAIGLGSLEFVLDEGQKNDWFGSNMILGFALASAFCLLALIPWELTR
                     DDPIIDLWLLGRRQFGACWLVMLGTGAVLISTTQFLPQILQTDFNYTAELAGLALSPG
                     GIATLIMMPIAGRLVGIVQPKYLIVLGASIVALAMWHLTGLNGDITYGYAVRARIILA
                     LGLPFLFLPVTTASYDGIPPDKTNQASALINVARNIGGSMGVALAQTIFAQRQQFHQS
                     RLVEHISSSDIGYQQTIDAMTRFFVGQGSNQPDAASQAIASIGQTLQRQIDLLSYIDV
                     FWSLAIIGALMVPLALIMRPIYLGVAPKGH"
     gene            complement(3572..4732)
                     /locus_tag="VN968_23475"
     CDS             complement(3572..4732)
                     /locus_tag="VN968_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HXL32227.1"
                     /translation="MDTQTRDRGPASEQAWPPERDPSTSDSAKSGEGVGERHEAQGPS
                     LSERLRQHWMLATVAACVVVVALVAGLLYWLDIRHYESTDDAFVAARSFSIAPKVGGY
                     VTEIPVTDNQHVTVGQLLARIDERDYQIAVNQAQAQADAAKANIANIEAQIASQQAQI
                     DQAKAQLDQAEAQLKFAEQEEARAQDLVQKGAGTVQKEQQTKSDLNAQQANTARAKSA
                     LIAAQLQDKVLATQRDSTRASLDQAQAQLDQAKLNLGYANIVAAQPGRVVKLSGAKGA
                     FVTPGQSLMMFVPDEIWITANYKETQLTDMRPGQQVEIRIDAYPNRKLNGHVASVQPG
                     SGTAFSLLPAENATGNYVKVVQRVPVKIVVDGWPAELPVGPGMSAVPWTRVR"
     gene            complement(5004..5240)
                     /locus_tag="VN968_23480"
     CDS             complement(5004..5240)
                     /locus_tag="VN968_23480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32228.1"
                     /translation="MHRLTENLSPADRRFYWKFVGSLFGFYGALMIIMVGVFFGNHLS
                     KNRALEPVMAETIGEKLPAGIEAPMPLRHAAKYD"
     gene            5529..5849
                     /locus_tag="VN968_23485"
     CDS             5529..5849
                     /locus_tag="VN968_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32229.1"
                     /translation="MTGSELKQIREDIGHAIGRSLSAADMAKLCGLAPGDGPDTIRKW
                     EVKGPSGPAAELLRILATASDRYPILENFNVFDRFNIDEKDRPARREAFRENMRDEVR
                     RRLG"
     gene            6204..6914
                     /locus_tag="VN968_23490"
     CDS             6204..6914
                     /locus_tag="VN968_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXL32230.1"
                     /translation="MKKFLLGTVALVALGAAAPAKAADLAARPYTKAPAYAAPIYNWT
                     GFYIGGHVGGAFAGDNSLTGNNGRFLGGVQGGFDYQFAQSWVLGAEAQYSWLSNNNNG
                     VVFPGGVLVTAKNDQLGSVTGRLGYTWGPALLYAKGGYAWKDGNNLNVSTGGVPVAFT
                     TNGNHRDGYTVGAGLEYMFAPSWSAKVEYQYYNFGSTMFTTGPAVIVGTSFRDDEHTI
                     KAGINYRFNWGGPVVARY"
     gene            7084..9231
                     /locus_tag="VN968_23495"
     CDS             7084..9231
                     /locus_tag="VN968_23495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HXL32231.1"
                     /translation="MALRSTMGKLRGLGFRRPKWGVRGSLFAAFAVIAGMAVVISAGA
                     GMVLGHLGGSMLDLSGRDIPRLAASLQLAAQSASLASQGPALLASGSEEALNERAKKM
                     KETQHDAVDKLGQIIELGADKTVVAALGETVKSIDDTIKSLGAAATERLGIAAQRERQ
                     YDALRKAQTAFAAAAAPAMIDAQTRIKAILGAANLLSQDDAIEAAQAIEQLGNVIAST
                     NLLASHMMAALSATNSDALEQVEKAFKAASPNVKSNLDSLTKNNDTAALKDAALKLLA
                     FGDGKSSVFKTRQKQLDADDYGQLILDETRKLNVGLRISVKQLVDGVQTETDASTWQA
                     RREISFATVVMLALGGLTFIGSVLFVWLYVGRNILRRIGNLQRSMQLLSGGDLDTEIH
                     RSRQRDEIAAMANSLEIFRGSMIEARALTAEQDKDRIVKAERASRMETRIVEFEATVR
                     AALDGLQTSAYSMQTTAQRMSATADQSSALVSAVASAAEETSTNVQTVSSGTEELSSS
                     ITEIGRQVTTSAEIARKAVDEAGQTDATMQGLAENASRISVVVDLIQVIASQTNLLAL
                     NATIEAARAGDAGRGFAVVASEVKSLANQTAKATDEIRAQIASMQQVTTTAVSAIRNI
                     GNTIGEINEVTTAIAAAVEEQGTATREIARNIQHAAGGTSEVSSNIVGVSTASAEAGS
                     AAGEVLNASGALRRETDVLRSEIDAFLSNIRAA"
     gene            9451..>9633
                     /locus_tag="VN968_23500"
     CDS             9451..>9633
                     /locus_tag="VN968_23500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL32232.1"
                     /translation="MDSKTDPTQSSAEALRYPWEHHPGPDQVVEVMPGVLWVRLALPF
                     RLNHVNIYLLADGDGWA"
BASE COUNT         1926 a         2999 c         2936 g         1772 t
ORIGIN      
        1 ccggtcggga ccggggtatg gccagcgttc tggatgctcg ccaatgacgg cggctggccg
       61 cccgaactcg acgtgatgga aggacgcggg caacagcccg gcgatctcgt gatgacgacg
      121 cattggcgca acaggaccac cggcctggtg caatcctgcg gcttcgattt ctcgaggccg
      181 gacgcatcga gcactttcca cgactatggc gtgctgtggg cgcaggacca ggtcgtctac
      241 ttcatcgacc gcaagccggc agcggatatc aagacccccg ttggattcga cgatcccatg
      301 tacatgatcg tgaaccttgc gatgggcgcg aagtatttca agggcgtcgg tttcgtcgac
      361 ggcatgtcgc ctgataccgt cgccttcgag atcgacagga tttccgctta tcaaatcgat
      421 ccctactcga agtaggcctg acgggagaac gcgatgttcg gtaaattctc gcgtcggcat
      481 tttgtcaagc tcgccggctt gtccgccttc ggcatggcgg cgacgccgac gaagcctgcg
      541 gaaagtgaaa caaaaccggc ggccgacggg catgcgccgg ccgcctttcc ggacggcttt
      601 ctatggggca cggccacgtc agcgtatcag atcgaaggcg cggtcgacga agacggccgc
      661 ggccgctcga tctgggacac cttctctcac acgccaggca agatcgagga tcacagcaac
      721 gccgaccgcg ccaacgacca ctatcatcgc tacaaggaag acgtcggcct gatcaaggaa
      781 ttgggggtca aggcctatcg tttttcgatc gcgtggccgc gggtgtttcc gcaaggaacc
      841 ggcgcgccga atcccaaggg tctcgacttc tacgatcgcc tgctcgatga acttctgaag
      901 gatggcatcg aaccgttcgc gacgctgtat cattgggatt tgccgcaagc gctgcaggac
      961 cgggttggcg gatggcaatc cagcgacacc tccagggcgt tcgcggatta cgcaggctat
     1021 gtggcggaac gtctgaccga tcgcgtcaaa aacattttca cgctcaacga agctggacga
     1081 ttcctgaact tcggttatgg atggggcatt gacgcccccg gtctcaaact gccgccagcc
     1141 gaactgaacc aggcacgtca ccacgtcgta ttggcgcacg gtctggccgt gcaggcgatc
     1201 cgcgcccatg ggcgcacggg gaccaaggtc ggcccggcgg aaaatatcgc ggcctgcgtg
     1261 ccggcgatcg atacgcccga gaacgttcgc gcagccgaga tcgcgacgcg cgaattgaac
     1321 gcgggttttt tgggcgtgat cctggagggc aaatacaccg acggttttct gcaatatgcc
     1381 ggcgccaatg ctcccaaatt taccgccgac gagctgaaga tcatttcctc gccgaatgat
     1441 ttcgtcggcc tgaacatcta cgcgccgcaa ttttacgtgg ttgcagccga ccgtgcgccg
     1501 ggcttcaagg ttctgccttt tccagcctcg tttccgcaca tgaattccga gtggctcagg
     1561 gtcggcccgg aaaccatcta ttgggtgccg cggctggctg caaaaatctg gagcatcgat
     1621 acgatctaca tcagcgagaa cggcacctct tcggccgacg atctggcggc cgacggcaac
     1681 gtctacgacc tcgatcgcat catgtacctg cgcaactatt tgacgcagtt gcagcgcgcg
     1741 acgtccgagg gcgtgccggt tcgcggctat ttcctgtgga gcctgatgga taatttcgag
     1801 tggatttttg gcttcgaaaa gcgctttggg ctgtaccgcg tagacttcga gacgcagcgc
     1861 cgggtcgcaa agctcagcgc gtcattctat cgcgacgttg tcgcgcgcaa tgcgatcggc
     1921 acttagatga taagccgctt ttcgccaggc ggattttatc aatgcccttt cggcgcgacg
     1981 cccaaataga tcggccgcat gatcagcgcc agcggcacca tcaatgcgcc gatgatcgcg
     2041 agcgaccaga agacgtcgat gtaggagagc agatcgatct gccgctgcag ggtctggccg
     2101 atcgaggcga tcgcctgcga ggccgcgtcc ggctggttcg atccctgacc gacgaagaat
     2161 cgagtcatcg cgtcgatggt ctgttgataa ccgatgtcgg acgaggagat atgctcgacc
     2221 aggcggcttt gatgaaattg ctgccgttgc gcaaatatcg tctgagccag cgcgacgccc
     2281 atcgagccgc cgatgttgcg cgcgacgttg atcagcgcgg aggcttggtt ggtcttgtct
     2341 ggcgggatcc cgtcatacga tgcggtggtg acgggtagaa acagaaacgg caggccgagc
     2401 gccagaatga tgcgagcccg cacggcgtag ccgtaggtaa tatcgccgtt cagtccggtc
     2461 aggtgccaca ttgccagcgc cacgatcgat gcacccagca cgatcagata tttcggctgg
     2521 acgatgccga ccaggcgacc ggcgatcggc atcatgatca aggttgcgat gccgcccggc
     2581 gacagcgcta atcccgccaa ctcggccgta tagttgaagt ccgtctgcag gatttgcggc
     2641 aggaactggg tcgtcgagat cagcaccgca ccggtgccga gcatcaccag ccagcacgcc
     2701 ccgaactgac gccggccgag cagccacaga tcgatgatcg gatcgtcgcg ggtcaattcc
     2761 caggggatca gcgccagcag gcagaaggcc gaggccagcg cgaaacccag aatcatgttc
     2821 gagccgaacc aatcgttctt ctgcccttca tcgagtacga attcgagcga gcccagtccg
     2881 atcgcgatca gcaggaaacc gacataatcg atgcgcacgc cgccgctgag caggcgggcg
     2941 cgctcctctt cggctcccga tggctcgctc accagcgtgc cgaccagaaa cagcgagatc
     3001 agtcccatcg gaacattgat caggaacacc caatgccagg aataggtgtc cgtgatccat
     3061 cctccgagcg tcggcccgac cacgggcgcg accacgacgg cgacgccata gatcgcaaag
     3121 gcctggccgc gcttggccgg cgggaatgaa tcggccagga tcgcctgctc gctggtcgcc
     3181 attccgccac cgcccaatcc ctgaatgatg cggaagaaaa ccagcgattg caggctccag
     3241 gccagtccgc acagcaacga agcgacggca aaagtggcaa cgcagatcat gtagaattgc
     3301 ttgcggccga tcacggtaga cagccagccc gaaatcgaca gcacgatggc gttggcgacg
     3361 agatagctgg tgatgacata ggtgctctcg tcgatgccga cggcgagccc gccggcgata
     3421 tggcgcaggg ccacgttggc gatagtggtg tcgagcacct ccataaaggt cgcgatcgaa
     3481 accacgaagg cgatcagata gggattgtga ccgcctggtg ccgatcgttc cggcgaccag
     3541 cctgaagaag ccgcggtctc cgccccggcg ctcatctcac cctggtccaa ggcaccgcgg
     3601 acatgcccgg accgacggga agctcggccg gccaaccgtc gacgactatc ttgaccggca
     3661 cgcgctgcac gaccttgacg tagttgccgg tcgcgttttc cgccggcagc aggctgaacg
     3721 cggtgcccga gccgggctgc accgaagcga catgaccgtt caatttccga ttcggataag
     3781 cgtcgatcct gatttcgact tgctgaccgg gccgcatgtc ggtcagttgg gtttccttgt
     3841 agttggcggt gatccagatc tcatccggca cgaacatcat caaactctgg ccgggcgtca
     3901 cgaacgcgcc cttggcaccg ctcagcttca ccacccgtcc cggctgcgcc gcgacaatgt
     3961 tggcgtagcc gagattgagc ttggcctggt cgagctgcgc ctgcgcctgg tccagcgacg
     4021 cgcgcgtgct gtcgcgttgc gtggccagca ccttgtcctg gagttgcgcg gcgatcagcg
     4081 cggacttggc gcgcgcagta ttggcctgct gcgcattgag atcggacttg gtctgctgtt
     4141 ccttctggac ggtgccggcg cccttttgca cgaggtcctg cgcccgcgct tcctcctgct
     4201 cggcgaactt caattgcgct tcggcctggt cgagctgtgc cttggcctgg tcaatctggg
     4261 cctgctggct ggcgatctgc gcctcgatat tggcgatgtt ggcttttgca gcatccgcct
     4321 gcgcctgcgc ctgattgacg gcgatctgat agtcgcgctc gtcgatacgg gccaacagtt
     4381 ggcccaccgt gacatgctgg ttgtcggtga cgggaatctc ggtgacgtag ccgccaacct
     4441 tgggcgcaat cgaaaagctg cgcgcggcga caaacgcatc gtcagtggat tcgtaatgcc
     4501 tgatatccag ccaataaagc aggccagcta cgagcgcgac gacgacaacg caggcggcga
     4561 cggtggccag catccaatgc tgccgcaatc tctcggacaa tgacggcccc tgcgcctcgt
     4621 ggcgctcgcc gacgccttcg ccgctttttg cagagtccga cgttgatgga tcgcgctcag
     4681 gcggccacgc ctgttcggag gcgggccccc ggtccctggt ctgagtgtcc acgcatcctc
     4741 ccgcgtcgaa aaagggtcca gccgatagcc ggaccaatgc ggcagttcga aaaacgggac
     4801 ggctaagagt ttgttcctgc gccactccac cgcggcggtg gaacgtccgg acgccagcgg
     4861 cgttatttct ggttcggcga tcaatacctt tgatccgcgg ggtccgatcc gacgcatcga
     4921 ctggcactct ggaagcttcg tctcgctgcc accccgcagg tcagcccttt cgcgcttatt
     4981 tcatgtgagg gcttcgtgga aaatcaatca tatttcgccg cgtgccgcaa aggcatcggc
     5041 gcttcgattc cggccggcag cttctcgccg atcgtctcgg ccatcacggg ttcaagcgcc
     5101 cggttctttg acagatgatt gccgaagaac acgccgacca tgatgatcat cagcgcacca
     5161 taaaaaccaa acagactgcc gacaaatttc cagtaaaatc ggcggtctgc ggggctgaga
     5221 ttctcggtca gtcggtgcat gtgcggctcc tcattgacga acgcaagcca gcataagctg
     5281 cacccgatga aaggcctgtg atcggcttca cacgcccgaa atcgtgatcc aggcccccat
     5341 tcagctccgt cgaatcagcg gatgtactcc gatacttctg ccagcggcac cggccttcgg
     5401 cggcttgatg gcgccaaccg tcggccaatt caaatcaaaa tattagcgag accggatccg
     5461 atcacggcta tgatttgccg gacacatcgg attcgaaaaa catcgggcga cgcgcaggat
     5521 cagcttgaat gaccggctct gagctaaaac aaattcgcga ggatatcggt catgcgatcg
     5581 gcagaagcct gtccgctgcc gacatggcga agctgtgcgg ccttgcgcct ggcgacggcc
     5641 ccgacacgat acgcaaatgg gaagtcaaag gccccagcgg gccggcggcc gagctgcttc
     5701 gcatactggc gacggcgagc gatcgatatc cgatcctgga aaatttcaac gtcttcgacc
     5761 gcttcaatat tgacgagaag gatcgccccg cgcgcagaga agcgttccgc gagaacatgc
     5821 gggacgaggt aaggcgccgg ctcggctaat cagcatcgcg tcccaaccct gcactgcggt
     5881 ttcgtgcatg ccggcgaatc atcagcgcgc tgctgttcgc ggcgctcggc tgcgcaacgt
     5941 ttcgcgatcg ttccgaattg catgggtgtg atgcatcagc aacgaactaa gcctccaagg
     6001 tgctcatacg cgagaaatta agccttgatt caggttccat tagcacttcg ttagtcacgg
     6061 gaaattgtag ttccatcaat aggttgggaa ctttctgact gtgacctatg ggtgacagca
     6121 ttttgtgcag aagctgtcta tctcccgcca tggatggggc cggcacacgg cgcctcggaa
     6181 ataatcgctg gagattaaca ctgatgaaga agtttctact tggcactgtg gccctcgtcg
     6241 cgctcggcgc ggccgcgccg gcaaaggcag ccgatcttgc ggctcgcccc tacaccaagg
     6301 ctccggccta cgccgcgccg atctacaact ggaccggctt ctacatcggc ggtcacgtcg
     6361 gcggcgcgtt cgcgggcgac aacagcctga cgggcaacaa tggccgcttc ctcggtggcg
     6421 tgcagggtgg tttcgattat cagttcgccc agagctgggt tctcggtgcc gaagcccagt
     6481 acagctggct gagcaacaac aacaacggcg tggtgttccc gggcggcgtt ctggtcaccg
     6541 cgaagaacga tcagcttgga tcggtcaccg gccggctcgg ctacacctgg ggcccggcac
     6601 tgctttacgc caagggcggt tatgcctgga aggacggtaa caacctcaac gtatccacgg
     6661 gcggcgtgcc ggtggctttc accaccaatg gcaatcatcg tgacggctac accgttggcg
     6721 ccggcctcga atacatgttc gccccgagtt ggtccgccaa ggttgaatac cagtattaca
     6781 acttcggcag caccatgttc acgaccggcc ccgccgtcat tgtcggcacc tcgttccgcg
     6841 acgacgagca taccatcaag gctggcatca actatcgctt caactggggt ggcccggtgg
     6901 tcgcgagata ctgatccgca acggattgaa agaagtctcg gaaaggccgg cctcgcgccg
     6961 gcctttttgt tttcgacgcg caaaatttgc gcagatcctg caaaagttcc cgattttatt
     7021 aacctggtaa cgcgatactg cccgaattca tcccggggat gaagcgagcg cgaggcagaa
     7081 agcatggcgt tacgatcaac gatgggcaaa ttgcggggcc ttggtttccg gcgcccgaaa
     7141 tggggcgtca ggggtagctt gttcgccgcc ttcgccgtga tcgcaggcat ggccgtcgtg
     7201 atcagcgccg gcgccggcat ggtgctggga catctcggcg ggtcgatgct cgatctgagc
     7261 ggacgggaca ttccgcgtct tgccgccagc ctgcaattgg cggcgcagag cgcaagcctt
     7321 gccagccagg gaccggcgct gctggcctcg ggcagcgaag aggcgctgaa cgaacgcgcc
     7381 aaaaaaatga aggagaccca gcacgacgcg gtcgacaagc tcggccagat catcgagctc
     7441 ggggccgata aaaccgtcgt cgcagcgctc ggtgaaaccg tcaagagcat cgacgacacc
     7501 atcaaaagcc tgggcgcggc ggccaccgaa cggctcggaa tagctgccca gcgcgagagg
     7561 caatacgatg ccctgcgcaa ggcccagacc gccttcgccg ccgccgcggc tcccgcgatg
     7621 atcgatgcgc aaaccaggat caaggcaatc ctgggggccg ccaatctgct ctcgcaggac
     7681 gatgccatcg aagccgccca ggcgattgag caacttggca acgtcatcgc cagcaccaat
     7741 ctgttggcct cgcatatgat ggcggcgctc tcggcaacca acagcgatgc gctcgaacag
     7801 gttgagaagg cgttcaaggc cgcatctccg aacgtcaaat ccaacctcga tagcttgacc
     7861 aaaaataacg acaccgccgc gctgaaggac gccgcgctga aattgctggc attcggcgac
     7921 ggcaagagca gcgtcttcaa gaccaggcag aagcaactcg acgccgacga ttatggccag
     7981 ctcattctgg acgaaacccg caagctcaat gtcggccttc gcatcagcgt gaagcaactg
     8041 gtcgatggcg tgcagaccga gaccgacgcc tcgacctggc aggcgcgccg ggagatttcg
     8101 ttcgcgaccg tggtcatgct tgcgctcggc ggcctcactt tcatcggatc cgtgctgttc
     8161 gtctggctct acgtcggccg caacatcctg cggcggatcg gcaatctgca acgctcgatg
     8221 cagcttttgt cgggcggcga tctcgatacg gaaattcatc gcagccgcca gcgcgacgag
     8281 atcgcggcga tggcgaattc gctggagatc tttcgcggga gcatgatcga ggcgcgcgcg
     8341 ctgaccgccg agcaggacaa ggatcgcatc gtcaaggccg aacgcgcctc ccgcatggag
     8401 acccggattg tcgaattcga agcaaccgtc cgcgccgcgc tggatggcct gcagacatca
     8461 gcctattcca tgcagaccac ggcgcagcgg atgtcggcca ccgccgacca gtccagtgcg
     8521 ctggtgagcg cggtggcgtc cgccgccgag gaaacctcga cgaacgtgca gacggtatcc
     8581 tcgggcacgg aggaattatc gtcatcgatc accgaaatcg gccgccaggt gaccacgtcg
     8641 gcggagatcg cccgcaaggc ggtcgatgag gccggccaga ccgacgccac catgcagggt
     8701 ctcgccgaga acgccagccg catcagcgtc gtggtcgacc tgatccaggt gatcgcctcg
     8761 cagaccaacc tgctcgcgct caacgccacc attgaggccg cgcgcgccgg cgatgccggc
     8821 cgcggctttg cggtggtcgc ctccgaggtg aagagcctcg ccaaccagac cgcgaaggcc
     8881 accgacgaga ttcgcgcgca gatcgccagc atgcagcagg tgacgacaac cgccgtcagc
     8941 gcgatccgca acatcggcaa taccatcggc gaaatcaacg aggtgaccac cgcgatcgcc
     9001 gccgccgttg aagaacaggg taccgcgaca cgcgagatcg cccgcaacat ccagcatgcc
     9061 gccggcggca cgtccgaggt ttccagcaac atcgtcgggg tctcgaccgc ctcggccgaa
     9121 gcgggctcgg ctgccggcga ggtgttgaat gcgtccggcg cgctccgccg tgaaaccgac
     9181 gtcctgcgtt cggagatcga cgcgttcctc tccaacatcc gggcggcgta gagaccgcgt
     9241 cattccggga tggtccgcag gaccagacct cagatgcgca attgcgcatc ggggaatctc
     9301 gagattccgg gttcgatgct tcgcatcgcc ccggaatgac ggtttccgcc cgcctccccg
     9361 gcgttttgcc aaaatcgcaa ttgttttcgg ctggcgtcgc gccgattgtg cggctaagcc
     9421 gataacgaca attttttgcg atatcaaacg atggattcga aaacagaccc cactcagtcc
     9481 tcggccgagg cgctgcgcta cccctgggaa catcatcccg gccccgatca ggtggtcgag
     9541 gtgatgcccg gcgtgttgtg ggtccgcctt gcgctgccgt tccggctcaa ccacgtgaac
     9601 atctatctgc tcgccgatgg cgacggctgg gcg
//
LOCUS       DATNOI010000582        15059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_224768,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000582 DATNOI010000000
VERSION     DATNOI010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15059
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224768"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            474..1244
                     /locus_tag="VN968_23505"
     CDS             474..1244
                     /locus_tag="VN968_23505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HXL32233.1"
                     /translation="MTQVEAQARNLNPVADRDVAARTRDVIARNPVLALWRTMIGKKV
                     VMAVTSVVLVAFVIVHMLGNLKMFSGPNEINAYSRFFREVGSPVFSYGQLLWLVRIIL
                     FISVTLHVIAAIQLTRMSWAARPIRYVVKRDIETTFDARLMRWGGVLLLAFIIFHLLH
                     LTGGIVGFAAGQFEHLNVYQNVVTAFSVWPVAFFYIVAMGALCLHLSHGIWSSLQTLG
                     WSTARSTATLKNLSRATAIMVFAGFSSIPVAVMAGWLR"
     gene            1256..3166
                     /locus_tag="VN968_23510"
     CDS             1256..3166
                     /locus_tag="VN968_23510"
                     /inference="COORDINATES: protein motif:HMM:NF005749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HXL32234.1"
                     /translation="MLNARIPSGLIEQQWDKCRFEMKLVSPANRRKHSIIVVGAGLAG
                     AAAAATLGQQGYRVQCFSYHDTPRRAHSIAAQGGINAAKNYRNDGDSIFRLFKDTVKG
                     GDFRAREANVYRLAQISVNIIDQCVAQGVPFAREYGGFLDTRSFGGVQVQRTFYARGQ
                     TGQQLLLGAYQALEEQVAAGTVTMHIRRELLDVVIVNGRARGIVTRDLVTGEIECHAA
                     DVIVLATGGYSNVFYLSTNAKASNCTAIWRAHRRGALFGNPCFAQIHPTCIPLSSEHQ
                     SKLTLMSESLRNDGRVWVPKKKGETRVANDIPENERDYYLERRYPSFGNLVPRDVASR
                     NAKEVCDEGRGVGPRGQGVYLDFADAIHRLGLETVHDKYGNLFDIYARITGENAYTVP
                     MRIYPAPHYTMGGLWVDYHLMSTIPGLFVGGEANFSDHGANRLGASALMQGLADGYFI
                     LPYTVPDYIASIALDPVDVSLHPFTAAEAEVKSRFEALVAVKGKRSPDSFHRELGNVI
                     WDNCGMARSDAGLRKALARIPELREEFWQNLRVVGGKDELNQTLEKANRISDFLEIAE
                     LMCVDALHRTESCGCHFRVESQTPEGEAKRDDANFSYTAAWEWKGPGKAPVLHKEPLT
                     FEYVPLSQRSYK"
     gene            3169..3984
                     /locus_tag="VN968_23515"
     CDS             3169..3984
                     /locus_tag="VN968_23515"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HXL32235.1"
                     /translation="MNLTLRIWRQQSANASGRFVTYEVKDVTRDMSFLEMLDALNEDL
                     LTQNAPPVAFEHDCREGICGSCGFLINGVAHGGQPGTTVCQLAMRFFKDGDSLTLEPW
                     RARAFPVIRDLIVNRSAFDRIIQAGGFISAPTGSAPDANAILVRREAADTAFDAAACI
                     GCGACVAACPNAAAMLFTSAKIAHLNALPQGQPQRDQRTLSMVVTMRNEHFGSCTNHG
                     ECEAVCPKKIPIEFIGTMNSDLIRATLRRHREPLVTPGVVQQPSHEEAAIEAE"
     gene            4154..4660
                     /locus_tag="VN968_23520"
     CDS             4154..4660
                     /locus_tag="VN968_23520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL32236.1"
                     /translation="MDGVSLERRLLVMAPLLSALAARGAEAGPIDQRETLVLPPDQIR
                     FQAWGNLPPASGEMAKLYGDFDEPGPYLVVMKWNPGWFSAPHSYRTDRIQVVVSGTWW
                     VNSGADFDPRQAVPVPAGGFVLRHARTSHYDGVPQDGNKPAVIAVFGIGPVDIRLADP
                     AKPSWRHV"
     gene            5026..6759
                     /gene="poxB"
                     /locus_tag="VN968_23525"
     CDS             5026..6759
                     /gene="poxB"
                     /locus_tag="VN968_23525"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HXL32237.1"
                     /translation="MKTVADQFSETIAAAGVKRIYGIVGDSLNGLTDAIRRQAKIEWV
                     HVRHEEVAAFAAGAEAHLTGALAVCAGSCGPGNLHLINGLFDCHRSRVPVLAIAAHIP
                     SAEIGAGYFQATHPESLFKECSHYCELVSSANQMPRTLEVAIREAVGRRGVSVLVIPG
                     DVALQRASDAPPPKLAGLLPPQPVTAPAAADLDRLAALLNGDSRVTILCGAGCAGAHL
                     ELLALGDRLKAPMVHAMRGKEHVEWDNPYDVGMTGLIGFSSGYYAMLDCDVLLMLGTD
                     FPYRQFYPSGGGVRIAQVDIRPEHIGRRTAVDLAVVGDVKATLQALLPRLDQKRDDTH
                     LTQAQLHYAKARRELDDLAVGSRRSDIIHPQQVAKAISDHAAEDAIFTCDVGLPTVWA
                     ARYLAMNGRRRLLGSFWHGSMANAMAQAIGAQKASPDRQVVSLSGDGGFTMLMGDFVS
                     LAQLNLPVKVVVFNNGTLGFVELEQKSTGFLDYGTELNNPDFAAMAQAIGIRGIRLTN
                     PADVDDGIANALAHDGPVLVDAVVARTELVMPPAITVEMAKGFSLYMVKAIMSGRGDE
                     IIDLAKTNLWR"
     gene            6771..8180
                     /locus_tag="VN968_23530"
     CDS             6771..8180
                     /locus_tag="VN968_23530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HXL32238.1"
                     /translation="MELTALLLSRIQWAFTISFHIIFPAFTIGLAAWLTVLEAMHFAT
                     GRPAYRLVFEFWLKIFGVAFGLGVVSGIVMAFQFGTNWSELSRISGPIQGPLLSYETF
                     TGFALEASFFGILLFGRSRVRPWFYLFSTAMVAFGTALSAFWIMTNNTWMQVPVGYAS
                     EQGMFAPIDWFKIVLNRVVWARFPHMVLAAFLTGAFCVAATGAWYLLRGRFAAEARIM
                     LNMGLYLAAILLPMQLFFGHLNGDYIHDFQPAKFAAIEARWHDEQPAAEVLIGIPDPA
                     TESNKYALTIPVLGSLIASLSFTSKEVGLTNFPPQDRPPVLIPFFAFRIMAGCWLIML
                     VLAWAGSYLIAKGRIEENRSLLWATFLSFPLPFIAILAGWFTAEVGRQPWTIYGVLRT
                     ADAVTPFLTARSAMISVLVYFAIYIFIFAFGSFYIHRLLRTGPVGHLVLPPTAAIPNR
                     PMSVVEPHTVDALHVSAGE"
     gene            8183..9169
                     /gene="cydB"
                     /locus_tag="VN968_23535"
     CDS             8183..9169
                     /gene="cydB"
                     /locus_tag="VN968_23535"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HXL32239.1"
                     /translation="MAMFWISVLAISILLYVLLDGFDLGVGMLFGVVRGEPQRHAMIS
                     AVAPIWDGNETWLVVSGVILWGAFPIVYSMLMSAFYLPVLLMLAGLILRGVAFEFRRK
                     TERMRWIWNASFAGGSLVAAFMQGVMVGALVEGLPLADGRYVGGEFGWLSPFAVLCGV
                     GLCVGYALLGACWLVRKCEGEVRDEAYRLIPYLSLGLLVFLVVVFAYALAENLAVMKR
                     WLERPYLFVFPVVGALAAIVLAASVRRRQDGPPFVMVALIFIAAFGTLALSFWPYMIP
                     FAITIADAAAPHSSLAFMFWGEGLFVFPLMLLYTAISLSVFRGKIRVAADHY"
     gene            9186..9491
                     /locus_tag="VN968_23540"
     CDS             9186..9491
                     /locus_tag="VN968_23540"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HXL32240.1"
                     /translation="MSNLQSVSGHLQNEDSDIRRRRLLFWCWHRGTQESDVMLGRFAE
                     AYLADFEARNSSGSKLCAIALTSMCSTGSSQRGVDASKGTRVAPRLPVQLRIPTNSF"
     gene            10122..10526
                     /locus_tag="VN968_23545"
     CDS             10122..10526
                     /locus_tag="VN968_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL32241.1"
                     /translation="MIRCVRLWTGDDGNSHYEEGTIDLSRGQRDDLLSDVLGGISLSF
                     QETKSGGSYEWHKDPVPRFVVTLSGTLEFETRGGERFTIRPGDILLAQDNTGTGHCWK
                     LIGDEPWRRAYLVFPTGAALPFKANSAHADSA"
     gene            10642..12171
                     /gene="mqo"
                     /locus_tag="VN968_23550"
     CDS             10642..12171
                     /gene="mqo"
                     /locus_tag="VN968_23550"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HXL32242.1"
                     /translation="MSTEADVVLIGAGIMGSTVGVFLKELEPSFSIAMIETLEDCALE
                     SSEAWNNAGTGHAANCEMNYTPERPDGTVDISTALEVNVEFDLSRQFWTYLVKKGAIA
                     DPRSFIHPVPHMSFVHDAGNVAFLRKRYAAMSAHHCYEGMEYSENRGQIAGWVPLLME
                     GRAADEPVAATRIISGTDVDYGALTHHLVHHLVGQPGCSVHYKTRVTNLVRADNKRWR
                     VEVQDLDSGERRSVTAKFVFIGAGGGALPLLQKSGIPEGHGYAGFPVSGIWVRCDDPA
                     VNKRHHAKVYGKAAVGSPPMSVPHLDTRVIGGQHSLLFGPYAGFSSKFLKHGSLFDLV
                     ESIRPANIEPLLAVARDNFDLTEYLIEQVLQSESHRLATLDAYYPKADPKDWRLQVAG
                     QRVQIIKPDVKRGGLLEFGTELVGAADSSLVALLGASPGASTAAFIAIGVLQKCFASE
                     LTEQAWLPKLKEIIPSYGISLIEDAAFTRKIRSETARALKIEDVNSKSMQAQDRTAAV
                     R"
     gene            12325..13737
                     /gene="fumC"
                     /locus_tag="VN968_23555"
     CDS             12325..13737
                     /gene="fumC"
                     /locus_tag="VN968_23555"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HXL32243.1"
                     /translation="MGSMRNETDSLGVVEVPSDKLWGAQTQRSLEHFSIGQDLMPREL
                     ITAYATLKKACAIANHDDERLDDQRYKLIVQACDEILAGNHHDMFPLHVWMTGSGTQF
                     NMNVNEVISNRCCELAGTPLGSHAPVHPNDHVNMAQSSNDTFPSAMYIAAAVNVKQRL
                     VPAVTALRDAIGAKAKAWDDIIKIGRTHMQDATPLTLGQEWSGYAGMLSDELERIEAA
                     LKGVYHLALGGTAVGTGINSAPGFAEAAAAEIAKLTGLPFVTAPNKFTVQGSHDALVQ
                     LSGTLRTLAVSLYKTANDIRLMSCGPRAGFAELEIPENEPGSSIMPGKVNPTQCEALT
                     MVAVQVMANDVAVGFGGAGGYLEMNVYKPLMIFNIAHSITIMTDGCTNFRKFLVEGTK
                     PNLKKINEYVERSLMLVTALSPVIGYDKASKIAHYAMDNDLTLKEAALKLGFVSEAEF
                     DRVVDPKKMVKPYVAGSTAH"
     gene            14001..>15059
                     /locus_tag="VN968_23560"
     CDS             14001..>15059
                     /locus_tag="VN968_23560"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HXL32244.1"
                     /translation="MLTAQVAADPASVEMDRVASAMRAIDAIEAGRLTADAAMESIAD
                     ISRATPAPTWLFALAAASGAAALAVIFGVQHMSAATLIFVSAGAGALLRRALGRLSRN
                     LFLQPFCAAALAGIVGALAVRYDLSSSLRLVAVCPCMVLVPGPHFLNGALDLINGRIS
                     LGAARLIYAGLIVVAISVGLLLGLGLLGVSLPTDPAGRAIPLWQDVIAAGVAVAAYSV
                     FFSTPLNMLPWPAGVGMFAHALRWAAITQLGVGVATGALVACVAVALALTPVARRTHM
                     PFAAIGFAAVVSMIPGVYLFRMASGLLQITSQSQTTLELLGATAADGLTAAIIILAMS
                     FGLIVPKMAIDYFSARFAR"
BASE COUNT         2798 a         4587 c         4618 g         3056 t
ORIGIN      
        1 cgacgtaggc atggacatcc tcggcccgcc actcagcgat gaagaagtcc gtcagatcat
       61 cgccgaagca gaggtctcgt agtcgaccga cgctcgacct agtctcgcgt gatgcgcttg
      121 ggcggctcgc ccttaccggc cgcgggctgc tgctggcccc agcgtgggag ggtgtagcgg
      181 gatgggcgcc gggcgtttct agagaatcag catgggccac gcgacaaaag cgacggcgcg
      241 gctctcttgt aggtcggctt ctcgacacgt tcgacaagga tgtagacggc gtggccgccc
      301 tgcaacacaa gtggaacagt cgattgcctt tgggtgcgcg agccgcctct ggccagtttt
      361 ggcgaaactc gagcgggggc ttccgcgttt tgacgcttgg ctcccattag cgcccggtat
      421 ccccacgcga tggattttcg ttttggacag accgaaaatg gaggatcccg atcatgaccc
      481 aagtcgaagc gcaagctagg aatttgaatc cggtggccga tcgcgacgtt gccgcgagga
      541 cgagagacgt gatcgcccgc aatcccgtat tggccctctg gcgcacgatg atcggcaaga
      601 aggtcgtcat ggcggttacc agcgtggttc ttgtcgcctt cgtgatcgtc cacatgctag
      661 gcaatctcaa gatgttctct ggtccgaacg agatcaacgc ttactcgcgg tttttccggg
      721 aagtgggttc tcctgtgttc agctacggac aactactgtg gcttgtgcgg atcattctgt
      781 tcatatccgt gactcttcac gtcatcgccg cgatccaact tactcgcatg agctgggctg
      841 cgcggcccat cagatacgtc gtcaagcgag atatcgagac gacgttcgac gcgcggttga
      901 tgcgctgggg cggcgtgctt ttgcttgcct ttatcatttt ccaccttctg cacctgaccg
      961 gaggcatcgt cgggttcgcg gcgggacagt tcgagcatct caatgtttac caaaatgtgg
     1021 tgacggcctt ctccgtctgg cccgtcgcgt tcttctacat cgtggcgatg ggcgcattat
     1081 gcctgcacct cagccacgga atctggagtt cgctgcaaac gctcggctgg agcaccgccc
     1141 gaagcacggc cacgctcaag aatctttcgc gtgccacagc gatcatggtg tttgcggggt
     1201 tcagctcgat tccggtcgcg gtgatggcag gctggctgcg ctaaaggaaa caaatatgtt
     1261 gaacgcaagg attccgtcag gtttgattga acagcagtgg gacaagtgtc gcttcgagat
     1321 gaagctggtg agtcccgcta accgccgcaa gcactcgatc atcgtcgtag gcgccgggct
     1381 ggctggagcc gcggccgccg ccactctcgg acagcaaggc taccgcgtgc aatgcttttc
     1441 ctatcacgac accccgcgcc gtgcgcactc gatcgcagcc caggggggga ttaatgctgc
     1501 caagaactat cgcaatgacg gcgacagcat tttccggctg tttaaggaca cagtcaaagg
     1561 cggcgacttc cgcgcgcgcg aggccaacgt gtatcgcctc gcccaaatca gcgtcaatat
     1621 catcgatcag tgcgtcgccc agggcgtgcc gtttgccagg gaatatggcg gatttctcga
     1681 tacacgatcg tttggcggcg tgcaggtcca acgaacattt tacgcgcgag gccagaccgg
     1741 acagcagctg ctgctcgggg cttatcaggc cctggaagag caggtggcag caggcacagt
     1801 gacaatgcat atcagaaggg agctgctcga cgttgtgatc gtcaacggaa gagcacgcgg
     1861 cattgtgacg cgagacttgg taaccggtga aatagagtgt catgccgcgg acgtgatcgt
     1921 gcttgcaacg ggagggtact cgaatgtctt ctacctctcg accaacgcca aggcgtccaa
     1981 ctgcactgcc atatggcggg cccatcgccg cggcgcccta ttcggaaacc cttgcttcgc
     2041 gcaaattcat ccgacatgca tcccattgag tagcgagcat cagtccaaac tgacgctgat
     2101 gtccgagtcg ctgcggaatg acggacgggt atgggtaccg aagaagaagg gtgaaacgcg
     2161 cgttgcgaac gacatcccgg aaaacgagcg cgactattat ctggagagac gctaccccag
     2221 ctttggcaac ctcgtacccc gcgacgtcgc gtcgagaaac gccaaggaag tgtgcgatga
     2281 aggacgcggc gtcggtccgc gcgggcaggg cgtgtatctg gatttcgcag atgccattca
     2341 taggctcggt ctggaaaccg tccacgataa gtacggaaac ctgttcgaca tttatgcgcg
     2401 gatcacggga gagaatgcgt acaccgtgcc gatgcggatc tatcccgccc cgcactacac
     2461 tatgggaggg ctgtgggttg attaccatct gatgtcgacc atcccaggac tgttcgtcgg
     2521 tggcgaagcg aacttctccg accacggagc caaccgcctc ggcgccagtg cgctcatgca
     2581 gggcctcgcc gacggctact tcattctgcc gtataccgtg cctgactaca ttgcctcaat
     2641 cgcgcttgac ccagttgacg tctcgctcca cccattcaca gccgccgagg ccgaggtcaa
     2701 gagtcggttt gaagcgctgg ttgctgtcaa agggaaacgt agccccgatt cgttccaccg
     2761 ggaactcggc aatgtgattt gggataactg tggcatggct cgctcggacg caggtctgcg
     2821 aaaggccctc gctcggatcc cggagttgcg tgaggagttc tggcaaaacc tccgtgtcgt
     2881 cggcgggaag gatgagttga accagactct cgagaaggcg aaccgcattt ctgactttct
     2941 cgaaattgcc gagctcatgt gtgtcgatgc gcttcaccgc acagagtcgt gcgggtgcca
     3001 cttccgcgta gagagccaaa caccagaggg tgaggcgaaa cgagacgacg cgaatttcag
     3061 ctatacggcg gcctgggagt ggaaaggtcc aggtaaggcg ccggtccttc ataaggaacc
     3121 gctcacgttc gagtatgtac ccctttcaca aaggagttac aaatagtcat gaatctcaca
     3181 ctacgtattt ggcggcagca gagcgcaaac gcgtcgggga ggttcgtcac ctatgaggtg
     3241 aaggacgtga caagggacat gtcattccta gaaatgcttg acgccctcaa cgaggacctc
     3301 ctcactcaga atgcacctcc cgttgccttc gaacacgatt gtcgggaggg tatctgcggc
     3361 tcgtgcggat tcctgatcaa cggcgtggct cacggaggcc aaccgggcac taccgtgtgc
     3421 caactcgcga tgcgcttctt caaggatggc gactcgctta cactcgagcc gtggcgggcc
     3481 cgcgccttcc cggtgatccg cgatcttatt gtgaatcggt cagcctttga ccgaataatt
     3541 caagcgggcg gctttatcag cgcgcccacg ggaagtgctc ccgatgctaa tgcaatcctg
     3601 gtccgaagag aagctgcgga taccgcgttt gacgcagccg catgcatcgg ctgcggagcc
     3661 tgtgtcgccg cctgtccgaa tgcggccgcg atgcttttca cttccgccaa aattgcccac
     3721 ctcaacgccc ttccgcaggg tcagccccaa cgcgaccagc ggactctcag catggtggtc
     3781 acgatgagga acgaacactt cggcagttgc acgaatcatg gtgagtgtga agcggtgtgc
     3841 cccaagaaga tacccatcga gttcatcgga acgatgaaca gcgatctgat tcgagcgaca
     3901 ttgcgccgcc accgcgaacc attggtcacg cccggcgtgg tacagcagcc atctcacgag
     3961 gaagccgcca tcgaagcaga ataggtcccg gctccggcgt ggtttcgcga aattccgagg
     4021 ccacaaccgt gcaagaacgc gaggacttgc acagcgtatt gcggcgttta tttgatgacg
     4081 cgcgtttcgc tgtagcaagc gcgacgtgtg cgcctcggcg gtaaacctga aagctgagaa
     4141 ggaggattcg ccgatggacg gtgtctcttt ggaacgacgt ctgctggtca tggcaccact
     4201 gctctctgca ctcgctgccc ggggggcaga ggccggtccg atcgaccaac gtgagaccct
     4261 cgtactgccg cccgaccaga tccggtttca ggcgtggggt aatctacctc ccgccagcgg
     4321 cgagatggcc aagctgtacg gcgactttga cgaacctggt ccttaccttg tggtgatgaa
     4381 gtggaacccg ggctggttca gcgcgcccca cagctacaga accgaccgaa tccaggttgt
     4441 ggtttcagga acatggtggg tcaacagtgg cgcggatttc gaccctcggc aggcggtacc
     4501 tgttccggca ggcggctttg tcctgcgcca cgcgcgtacc tcgcactacg acggcgtgcc
     4561 ccaggatgga aacaagccgg ctgtgatcgc cgtctttggg atcggtccgg tcgatatccg
     4621 gctggcggat cctgcaaagc catcctggcg gcatgtttag acaccggcgc ggcctcgcgc
     4681 gggaggtcct tgtttttggg gacttttgaa gccctttgga gcttctgctt ccgcgcggcc
     4741 ggaactctag ccagcacggc gttagaataa agccgatttc agtttttgga actttcttgc
     4801 gcgaaatcta ttttattatt cgacaggcaa tttcatagaa cggcgcttcc tccggcgtat
     4861 ttccgacagt aaccgcattc gtacacgcgg gggaagtaca ggagggagtg agcctcggtc
     4921 tgaaccctgc cgaacattac cttcgcatga cagtgacggc gtgatcccaa gcaatccggg
     4981 gccctggttt tcttacaaca tgatgaataa tatgaaaaaa acaatatgaa aactgttgca
     5041 gaccaatttt ctgaaactat tgccgcggcg ggtgtcaaac gaatctatgg tattgttggc
     5101 gacagcctca acggcctgac cgatgccatt cgccgccaag ccaagatcga gtgggtgcat
     5161 gtccgccacg aggaggtggc cgccttcgcg gccggcgccg aagcgcatct gaccggagcg
     5221 ctcgccgtct gcgccggcag ctgcgggcca gggaacctgc acctcatcaa cggactgttc
     5281 gattgccacc gctcgcgcgt gccggtgctc gccattgcgg cgcatatccc ctccgctgag
     5341 atcggcgccg gctacttcca ggcaacgcac cccgagagcc tgttcaagga atgcagccac
     5401 tactgcgagc tcgtttccag cgccaaccag atgccgcgca cgctggaggt ggctatccgc
     5461 gaggcggtcg gcaggcgcgg cgtttcggtc ctggtaatcc ccggcgatgt cgcgctgcag
     5521 cgggcctctg acgcgccgcc acccaagctc gcaggtttgc tgccgccaca gccggtgaca
     5581 gcgccggcag cggcggacct cgaccggctg gcggcgctgc tcaatggcga cagccgtgtg
     5641 accatcctgt gcggcgccgg atgcgcaggc gcgcacctgg agctgttggc gctcggtgac
     5701 cggctcaagg cgccgatggt gcacgcgatg cgcggtaagg aacacgtcga gtgggacaat
     5761 ccctacgacg tcggaatgac ggggctgatc ggtttttcgt ccggctacta cgccatgctg
     5821 gactgcgatg tgctgctcat gctcggcacg gacttcccat accggcagtt ctatccgagc
     5881 ggaggcggcg ttcgcatcgc gcaggtcgat atccggccgg agcatatcgg acgccgcacg
     5941 gcggttgacc ttgcggtggt cggagatgtc aaggcgacgt tgcaggcgct gctgccgcgc
     6001 ctcgatcaaa agcgcgacga cacgcatttg acgcaggcgc agctgcacta cgccaaggcg
     6061 cgccgtgagc ttgacgatct cgccgtgggc agccggcgaa gtgatatcat ccatccacag
     6121 caggtcgcca aggcgatcag cgaccatgct gccgaagacg ccatctttac atgcgatgtc
     6181 ggcctgccga ccgtttgggc agcacgctac ctcgccatga atggccgccg gcggctgctg
     6241 ggatcgttct ggcacggctc gatggcaaac gcgatggcgc aggcgatcgg cgcccagaag
     6301 gcgtcgcccg accgtcaggt tgtgtcgctg tcaggtgacg gcggcttcac catgctgatg
     6361 ggtgatttcg tcagcctcgc gcaacttaat ctgcctgtga aggttgttgt tttcaacaac
     6421 ggcacgctcg gtttcgtcga gcttgagcag aaatccacgg gctttctcga ctacggaacc
     6481 gaactcaaca atccggactt cgcagcgatg gctcaggcga tcggtatccg cgggatccgg
     6541 ctcacaaatc ccgctgacgt cgatgacgga attgccaacg cgctggcgca tgatggtccg
     6601 gttcttgtcg acgccgtcgt cgcgcggacc gagcttgtca tgccgcccgc gatcaccgtc
     6661 gagatggcga agggtttctc gctctacatg gtgaaggcga tcatgagcgg tcgcggcgat
     6721 gagatcatcg atctcgcgaa aaccaatctg tggcgctgag gaaattggcc atggagctca
     6781 cagcgcttct cctgtcccgg atacagtggg cgttcacgat ttcgtttcac atcatctttc
     6841 cggcgttcac catcgggctc gcagcatggc tcacggttct ggaggcgatg cacttcgcaa
     6901 ccggtcgccc cgcctatcga ctagtttttg aattctggct gaagattttc ggcgtggcgt
     6961 tcgggctcgg cgttgtctcc gggatcgtca tggcgttcca gttcggcacc aactggagcg
     7021 agctgtcgcg gatatccgga cccatccagg gcccgctctt atcctacgag accttcaccg
     7081 gcttcgccct ggaggccagc tttttcggaa tcctgctgtt tggccggtct cgcgtgcggc
     7141 cgtggttcta cctgttttcg accgcgatgg tcgcattcgg caccgcactt tccgcgttct
     7201 ggatcatgac caacaacacc tggatgcagg tgccggtcgg ctacgccagt gagcagggca
     7261 tgttcgctcc gatcgactgg ttcaagatcg tcctcaatcg ggtcgtctgg gcacgcttcc
     7321 cccatatggt tctcgcggcg ttccttactg gcgcattctg cgtcgccgca accggcgcat
     7381 ggtacctgct gcgcggcagg tttgccgcgg aggcgaggat catgctgaac atgggtctgt
     7441 accttgcagc aatactcctg ccgatgcagc tcttcttcgg gcatctcaac ggcgactaca
     7501 tccatgattt tcagccggcc aaattcgccg ccatcgaagc ccgctggcat gacgagcagc
     7561 cggccgccga ggtcctcatt ggaattccgg atccggcgac ggagagcaac aaatatgcgc
     7621 tcacgatccc tgtcctgggc agcctgatcg caagcttgag tttcacctcc aaggaggtgg
     7681 gcctgaccaa ctttccaccc caggaccgac ctcctgtgct catcccattc tttgcattcc
     7741 gtatcatggc gggctgctgg ctgatcatgc tggtgctcgc ctgggccggg tcgtatttga
     7801 tcgccaaggg ccgtatcgag gaaaatcgat cgctgctgtg ggcgacgttt cttagctttc
     7861 ccctgccgtt cattgcgatc ttggccggtt ggttcacggc cgaggtaggc cgccaaccct
     7921 ggaccatcta cggcgtactg aggaccgcgg acgccgtgac gccgttcctg acagctcgct
     7981 cggcgatgat ctcggtcctc gtctatttcg caatctacat cttcatcttt gctttcggca
     8041 gtttctacat ccaccggctg ctccgcacgg gaccggtcgg gcatctcgtt ctgcccccca
     8101 cggccgctat ccccaaccgg cccatgtcgg tcgtcgagcc gcacaccgtg gacgctcttc
     8161 acgtatcggc gggggaatag ccatggccat gttctggata tcggtgctcg ccatcagcat
     8221 tctcctctac gtcctgctcg acggattcga cctcggcgtc ggcatgcttt tcggcgtagt
     8281 gcggggcgag ccgcagcggc atgccatgat aagcgcggtg gcgccgatct gggacggcaa
     8341 tgagacctgg cttgtcgtct cgggcgtgat cctgtgggga gcgtttccga tcgtctattc
     8401 aatgctgatg tcggcgttct acctgccggt tctattgatg ctggcggggc tgatcctgcg
     8461 tggcgtcgcg ttcgagtttc ggcgcaaaac cgagcggatg cgctggattt ggaatgcgag
     8521 ctttgccgga gggtcgctcg ttgcggcatt catgcaaggc gtcatggtcg gggccctggt
     8581 ggagggcctg ccgctcgccg acggtcgcta cgttggcggc gaattcggtt ggctcagccc
     8641 ctttgcggtg ctctgtggcg ttggcctgtg tgtcggctac gcgctcctcg gcgcctgctg
     8701 gctcgtcaga aaatgtgaag gcgaggtccg cgacgaagcc tatcgcctta tcccctatct
     8761 gtcgcttggg ctactcgtct tcctggtcgt cgtgtttgcc tatgccctgg ccgaaaatct
     8821 ggcggtcatg aagcgatggc ttgagcggcc atatttgttt gttttccccg tcgtcggcgc
     8881 ccttgccgcg atcgtgctcg ccgcaagcgt tcgccgccgt caggacggac cacccttcgt
     8941 catggtggct ctcattttca tagcggcatt cgggacgctc gcgctctcgt tctggcctta
     9001 catgatcccg ttcgccatca ccatcgcgga cgccgcggcc cctcactcca gccttgcctt
     9061 catgttctgg ggtgaagggc ttttcgtctt cccactcatg ctgctctaca cggcgatcag
     9121 cctgagcgtc ttcagaggca agatcagagt agcggcggac cactattaga acggaaagac
     9181 ttgccatgag caatctccaa agcgtctccg gccacttgca gaacgaggat tccgatatcc
     9241 gccgcaggcg cctcttgttc tggtgctggc accgcggaac gcaggagagc gatgtcatgc
     9301 tcggcagatt tgccgaggcc tatctcgctg acttcgaagc gcgcaactcg agcggttcga
     9361 agctttgcgc gattgcgctg acatcgatgt gttcgactgg atcatcccag cggggcgttg
     9421 acgcgtccaa aggcacaagg gtcgcgcccc gactccctgt gcaactacgg ataccaacaa
     9481 acagcttctg aggtgttcat ttaatcagaa ggcaccccaa tggttcgaac ccaccagcca
     9541 ggccaagaag ttttcagatc gatcctgtcg gaaagcattg actggaagcc gttctcagcg
     9601 tttccgccgg cggcccggct ggccgtgctt ttggagagcc gagtcaaccg ggaccttacg
     9661 tcattagagt caaagtgcca tctggcgtgc ggctgatgcc gcacagacac ccggaggacc
     9721 gaatctacac agtgatatcc ggcgtcttct acatcggcct gggatctcag ttcgacggcg
     9781 acaaggtgca ggcgtatccg cctggtagcg tgattatctt gcccggcgaa cggcgatcca
     9841 tgtcaccgcc gcggtgggga cacctagcgc aatgatcgaa tggcgtagtt tcgatctcga
     9901 agcacagctt tatagcggcg cgctcgctcc ggagggcggc cggatcttag tgcctcaaag
     9961 tccgggtttg ggaatggagc ctgaccccgg cgtaattggc gcctatttga gagtctgatc
    10021 tacctaccgg attgggcacg cagactcaac gtttgctcgt ctatttctcg accggcaacg
    10081 gcggccaccg ccgcgcggtg cctgactgaa aggaagacat catgatccgg tgtgtgagac
    10141 tgtggaccgg cgacgatggc aactcgcatt atgaggaagg aacaatcgat ctttcccggg
    10201 gccagagaga cgaccttctg agcgacgtgc tcggcggcat cagtctgtcc ttccaggaga
    10261 ccaaatccgg gggatcctat gagtggcaca aggatccggt gccgcgcttt gtcgtcacgc
    10321 tcagcggtac actggaattc gagacgcgcg gtggcgagcg cttcaccatc cgtccgggtg
    10381 acatcctgct ggcgcaggac aacacgggta cgggacattg ctggaagctg atcggcgatg
    10441 aaccttggcg gcgcgcctac ctagtgttcc cgacgggcgc cgcgcttccg ttcaaagcca
    10501 attcggccca cgcggattcc gcatgaccaa aggcagggag gtcgcgaagg gcgtttagcc
    10561 gccttggatc cgcgccgcgg ctgaacgcca tcagacgcga caaaacaagg cgaggtcttc
    10621 acaaaacctg gagaggccag catgagtacc gaagcggacg tcgtcctgat tggcgcaggc
    10681 attatgggct cgaccgtcgg cgttttcctg aaagaactgg agccgtcgtt ctccatcgcc
    10741 atgatcgaga cgttggagga ttgcgcgctc gagagctcgg aagcctggaa caacgcaggt
    10801 accggccacg ccgccaattg cgagatgaac tacacgcccg aacggccgga cggcaccgtc
    10861 gacatctcca cagccctgga ggtcaacgtc gagttcgatc tgtcgcggca gttctggacg
    10921 tacctggtga agaagggcgc catcgccgac ccgcgcagct tcatccatcc ggtgccgcat
    10981 atgagtttcg tgcacgacgc cggcaacgtc gcgtttctca ggaagcggta cgccgcgatg
    11041 tcggcccatc attgctatga gggcatggag tacagcgaga accgcggcca gatcgccggc
    11101 tgggttccgc tgttgatgga aggccgcgcc gccgatgaac cggtcgcggc cacgcgcatc
    11161 atctccggca ccgacgtcga ctatggcgcg ctcacgcacc atttggtgca ccacctcgtc
    11221 ggccagcccg gttgcagcgt gcactacaag acccgcgtca ccaacctcgt ccgcgccgac
    11281 aacaagcgct ggcgcgttga ggtgcaggac ctcgacagcg gtgagaggcg ctcggtcacc
    11341 gccaaattcg tcttcatcgg agcaggcggt ggcgcgctgc cgctgctgca gaaatccggc
    11401 attccggaag gccacggtta cgccggcttc cccgtgagcg gaatctgggt gcgctgcgat
    11461 gatcccgcgg tcaacaagcg ccaccacgcc aaggtctacg gcaaggccgc ggtgggctcg
    11521 ccgccgatgt cggtgccgca tctcgacacc cgcgtcatcg gcggacagca ttcgttgctg
    11581 ttcggtccct atgctggctt ctccagcaaa ttcctgaagc acggctcgct gttcgatctc
    11641 gtcgagtcga tccgtcccgc caatatcgag ccgttgctcg cagtcgcccg cgacaatttc
    11701 gacctgaccg aatacctcat cgagcaggtg ctgcagtccg aaagtcaccg cctggcgacg
    11761 ctggacgcgt actacccgaa ggcagatccg aaggattggc ggctgcaggt cgccggacag
    11821 cgcgtccaga tcatcaagcc cgacgtcaaa cgcggcgggc tcctggaatt cggcaccgag
    11881 ctcgtcggcg cggcggattc ttccctggtg gcgctgctcg gcgcctcgcc tggcgcctcg
    11941 acggcggcct tcatcgcgat cggcgtgctg caaaagtgtt ttgcgagcga actgacggag
    12001 caggcgtggc tgccgaaact gaaggagatt atcccttcct atggcatttc gctgatcgag
    12061 gatgccgcct tcacccgcaa gatccgttcc gagaccgcgc gggctctcaa aatcgaggac
    12121 gtgaactcga agtcgatgca ggcacaggat cgaacggcgg ccgtccgata aagtcgcggg
    12181 caatgtggcg caagtcctgc aagcccggct acagctgaag cgcagcgcaa aagtgcgatt
    12241 ttgttgagcg gattcaggcg ccaagagcga gccgcttgca gaaatcaagc catttagtga
    12301 ccagcgcata atggagacgt caaaatgggt agcatgagaa acgagaccga cagcctcggt
    12361 gtggtcgaag tgccctcaga caagctgtgg ggtgcgcaaa cccagcgctc gcttgagcat
    12421 ttcagcatcg gccaggatct catgccgcgc gagctgatca cggcttatgc gacgttgaag
    12481 aaagcctgtg ccatcgccaa ccatgacgac gagcggctcg acgatcagcg ctacaaattg
    12541 atcgtccagg cctgcgacga aatcctggcc ggcaatcacc acgacatgtt tccgttgcac
    12601 gtctggatga ccggcagcgg cacgcagttc aacatgaacg tcaatgaagt gatctccaac
    12661 cgctgctgcg agctcgctgg cacgccgctc ggcagccacg cgccggttca tcccaacgat
    12721 cacgtcaaca tggcgcaatc gtcgaacgat actttccctt cggcgatgta catcgccgcc
    12781 gccgtcaacg tgaagcagcg cctcgttccg gcggtgactg cgctgcgcga tgccatcggt
    12841 gccaaggcca aggcgtggga cgacatcatc aagatcgggc gcacccatat gcaggacgcc
    12901 accccgctga ccctggggca ggaatggtcc ggctatgccg ggatgttgtc ggacgagctg
    12961 gagcgcatcg aagcggcgct taagggcgta taccacctgg cgctcggcgg caccgcggtc
    13021 ggcacgggca tcaactcggc gcccggcttc gccgaagcgg cagcggccga aatcgcgaag
    13081 ctgaccggtc tgcccttcgt caccgcgccc aacaagttca cggttcaggg ctcccacgat
    13141 gcgctggtgc agctttccgg aacgctgcgg acgctggcgg tctcgctcta caagaccgcc
    13201 aacgacatcc gcctgatgtc gtgcgggccc cgtgccggat ttgccgagct ggagatcccc
    13261 gagaatgagc cggggtcttc gatcatgccc ggcaaggtca atccgaccca gtgcgaggca
    13321 ctcaccatgg tcgcggtgca ggtgatggcc aatgacgtgg ccgtcggctt cggcggcgcc
    13381 ggcggttacc tggaaatgaa tgtctacaag ccgttgatga tcttcaacat cgcgcactcg
    13441 atcaccatca tgaccgacgg ctgcaccaat ttccgcaagt tccttgtcga gggcaccaag
    13501 ccgaacctga agaagatcaa cgaatatgtc gagcgctcgt tgatgctggt gacggcgcta
    13561 tcgcccgtca tcggctacga caaggcctcg aagatcgccc actacgccat ggacaacgac
    13621 ctcacgctga aagaggccgc gttgaaactg ggctttgtca gtgaggcgga gttcgatcgc
    13681 gttgtcgacc ccaagaaaat ggtgaagccg tacgtggcgg gttccaccgc gcattgagcc
    13741 actgaacgcg ttgcggccgg cacactcgac gccggctgca accgcgcggc agatccgacg
    13801 catcgactgt gggaagattc gatgactgcg gatgagcgat ctcgattggt ccttgctttt
    13861 gccagggttc tctacgtcaa cggacaagcc acggaacgga ccgtgagcgc ggccgagcgc
    13921 cttgctcggg cgctgggact gcgcgcgacc atcgtgccgc gctagggtgg ctggagcttc
    13981 tggtcgacga ccagcagggc atgctgacgg cccaggtggc ggccgatccg gcgagcgtgg
    14041 aaatggatcg cgtggcttcg gcgatgcggg cgatcgacgc tatcgaagca ggtcgcctca
    14101 cggcggatgc cgctatggag agcatcgcag acatctcgcg cgcgacaccg gcgccgacat
    14161 ggctcttcgc gctcgccgcg gcgtccggcg cagcggcgct ggcggtgatc ttcggcgtcc
    14221 agcatatgtc ggccgcgact ctgatatttg tgagcgccgg tgccggcgca ttgttgcgcc
    14281 gcgctttggg ccgactgagc cgcaatctct tcctgcagcc gttctgcgcg gcggcccttg
    14341 ccggcatcgt cggggcgctg gccgtccggt acgacctgag ttcatcgttg cgcctcgtcg
    14401 cggtctgccc gtgcatggtg ctcgtgcccg gtccgcattt tctcaatggc gcgctcgacc
    14461 tcatcaacgg ccgcatatcg cttggcgccg cccgcttgat ttatgccggg ctgatcgtcg
    14521 tcgcgatctc ggtaggattg ctccttgggc tggggctgct cggcgtatcc ttgccgaccg
    14581 acccggctgg cagagccatc cccctgtggc aagacgtcat tgccgccggc gttgccgttg
    14641 ccgcatacag cgtgtttttt tcaacgccgc tcaatatgct gccgtggccg gctggcgtcg
    14701 gcatgttcgc ccacgctttg cgatgggcgg ctatcacgca gcttggcgtt ggcgtggcga
    14761 ccggcgccct ggtcgcttgc gttgccgtgg cgttggccct cacccctgtc gcacggcgaa
    14821 cgcatatgcc gtttgccgcc atcggcttcg ccgccgtggt gtcgatgatt cctggcgtct
    14881 acctcttcag gatggcgagc ggcctcctgc agatcacaag ccagtcgcaa acgacattgg
    14941 agctgctcgg cgcgacggcg gccgacgggc tgaccgccgc gatcatcatc ctggcgatga
    15001 gttttggcct cattgttccg aagatggcca tcgactattt cagcgctcgt tttgcgcgg
//
LOCUS       DATNOI010000583         4124 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_426072,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000583 DATNOI010000000
VERSION     DATNOI010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426072"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="VN968_23565"
     CDS             <1..1408
                     /locus_tag="VN968_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HXL32245.1"
                     /translation="FYLFSKLEFLGRNDGISGIPPLTIGPLKMLDPGTIYFAIWIAVL
                     IAALLTMNLLDSRTGRAIRALRRGHIAAEAFGVQTPRAKLLVFIYAAVLAGLSGWLYA
                     HFQRAANPTPFGAQAGIEYLFIAVVGGAGYVWGGVLGAGIVVILKEILQSYLPYLFHG
                     EGQLETIVFGILLVVLLQLAPTGVWPWLMARLPFKPKRRPPDTSLTLPARKRTADVPA
                     VLLQIDNTRKQFGGVIAVNNVSFDVQAREIVALIGPNGAGKSTTFNLITGVLTSSGGT
                     ISVLGRNINNAPPQEVAKLGIARTFQHVKLVPDMSVLENVAIGAHLRGHAGAISSMLR
                     LDRSDEARLLAEAARQIERVGLGDQIDAMAGSLSLGQQRIVEIARALCVDPILLLLDE
                     PAAGLRHMEKQQLATLLRQLRDGGMSVLLVEHDMGFVMDLADRIVVLDFGTKIAEGTP
                     QAIKTNPEVIKAYLGAVA"
     gene            1417..2148
                     /locus_tag="VN968_23570"
     CDS             1417..2148
                     /locus_tag="VN968_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32246.1"
                     /translation="MSALLSVSDAHVAYGKVEAVRSVSLDVGDHEIVTIIGANGAGKT
                     TLLNAIMGILPLKGRATFAGEDVLRLEIEDRVASGLCLVPEHRELFSTMNVEDNLQLG
                     AFRIAKAATAVAFERVYTLFPRLKERRKQLAGTLSGGEQQMLAMGRALMGAPRLLMLD
                     EPSLGLAPIIVADIFRTIGELRESGVSVLLVEQNAQAALQIADRAYVMELGEFILNGP
                     AKDIATNQRVAASYLGFQHEGASGI"
     gene            complement(2252..3406)
                     /locus_tag="VN968_23575"
     CDS             complement(2252..3406)
                     /locus_tag="VN968_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32247.1"
                     /translation="MKKFYLSAAVIAASLGLPGLPALAQSNEIVIGITVTTTGPAAAL
                     GIPERNALEFVPKEIGGVPVKVIVLDDGGDPTAATTNARRFVTESKADIIMGSSTTPP
                     TVAVSTVANEAGIPHFGLAPFPITPERLKWSVGMPQPVPIMGKVLYEHMKAHNIKTVG
                     YIGYSDSYGDLWFNDFKTQAVPMGMTLAAEERFARPDTSVAGQVLKLVAANPDAVLVG
                     ASGTAAALPQTALRERGYKGLIYQTHGAASMDFIRIAGAAAEGVIMASGPVMNPEGQP
                     DSALTKKPGLALNAAYEAKYGPNSRSQFAGHSYDAFEVLKRVIPVALKAGKPGTPEFR
                     EGIRQAWLSERDIAASQGVYNFTEKDRYGLDDRSRIILTVKNGKYIAASE"
     gene            complement(3512..3931)
                     /locus_tag="VN968_23580"
     CDS             complement(3512..3931)
                     /locus_tag="VN968_23580"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXL32248.1"
                     /translation="MFVNRRDVQIQWGDCDPANIVYYPRYFEMFDDSTSILFEAAGFS
                     KQDIIKKYGLVGIPMVDTRAKFYIASTHGDWITIESQIESFKRSSFEVTHKVFKGEAL
                     AIEAFETRVLVGRHPDDPARLKSAAVPEEIIARFQRG"
     gene            complement(3940..>4124)
                     /locus_tag="VN968_23585"
     CDS             complement(3940..>4124)
                     /locus_tag="VN968_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HXL32249.1"
                     /translation="TPLLANLPQEAQDSLAASIPFPRRLGHADEFASLALHMIDNPYL
                     NGEVVRLDASLRMAPR"
BASE COUNT          730 a         1314 c         1294 g          786 t
ORIGIN      
        1 gttctatctg ttcagcaagc tcgagttcct cggtcgcaat gacggcatct ccggtatccc
       61 gccgctgacg atcggcccgt tgaagatgct cgaccccgga acgatctatt tcgcgatctg
      121 gatcgcggtg ctgatcgcag cactgcttac catgaacctt cttgactcgc gcaccggccg
      181 cgcgatccgt gcactgcggc gcggacacat cgccgccgag gcgtttggcg tgcagacgcc
      241 gcgcgccaag ctcctggtct tcatctatgc ggcggtgctg gccggcctgt ccggctggct
      301 ctatgcgcat ttccagcgtg cggccaatcc gacgccgttc ggcgcgcagg ccggcatcga
      361 atatctcttc atcgcggtgg tcggcggcgc cggttacgtc tggggcggcg tgctcggtgc
      421 cggcattgtc gtgatcctga aggaaatcct gcagagctat ctgccctatc tgtttcacgg
      481 cgaaggccag ctcgagacca tcgtgttcgg catcctgctg gtggtgctgc tgcaactggc
      541 cccgaccggc gtctggccct ggctgatggc gcggttgccg ttcaaaccaa agcgcaggcc
      601 gcccgataca tcgctgacgt tgcctgcgcg aaagcgaacg gcggacgttc ccgccgtgct
      661 cctgcagatc gacaatacgc gcaagcagtt cggcggcgtg atcgccgtca acaacgtgtc
      721 tttcgacgtc caggcccgcg agatcgtcgc gctaatcggc cccaacggcg cgggcaagag
      781 cacgaccttc aacctgatca ccggcgtgct cacttcatcg ggcggcacga tctccgtgct
      841 cggccgaaac atcaataacg cgccgccgca ggaagtcgca aaacttggca tcgcccgcac
      901 cttccagcat gtcaaactgg tgccggacat gagcgtgctg gaaaacgtcg cgatcggcgc
      961 gcatctgcgc ggccatgccg gcgcgatctc cagcatgctc cgcctcgatc gatccgatga
     1021 agcgagattg ctggcggaag cggcgcgcca gatcgagcgc gttggcctcg gcgaccagat
     1081 cgacgcgatg gcgggcagcc tgtcgctcgg ccagcagcgc atcgtcgaga tcgcccgcgc
     1141 gctgtgtgtc gacccgatcc tgctgctgct cgacgagccg gccgccggcc tgcggcatat
     1201 ggagaagcag cagcttgcaa ccctgctgcg ccagttgcgg gacggcggca tgtcggtgct
     1261 gctggtggag cacgatatgg gatttgtgat ggacctggcc gaccgcatcg tggtgctcga
     1321 cttcggcacc aagattgccg aaggcacgcc acaggcgatc aagaccaatc ccgaggtgat
     1381 caaggcctat ctcggagcgg tcgcatgaat cctggtatca gcgcgctgct gtcggtgtcc
     1441 gatgcacacg tcgcctatgg caaggtcgag gcggtgcgtt cggtgtcgct cgatgtcggc
     1501 gaccacgaga tcgtcaccat catcggcgcc aacggcgccg gcaagaccac gctgttgaat
     1561 gccatcatgg ggatcctgcc gctgaagggc cgcgcgactt tcgctggcga ggatgtattg
     1621 cggcttgaga tcgaggatcg cgtcgcgtcc ggcctctgtc tggtgccgga gcaccgcgaa
     1681 ctgttcagca ccatgaacgt cgaggacaat ctgcaactcg gcgcgttccg gatcgccaag
     1741 gcggccacgg ctgtggcgtt cgagcgggtc tacacgcttt ttccgcggct caaggaacgg
     1801 cgcaagcaac tggcgggaac gctatcgggc ggcgagcagc agatgctggc gatgggccgc
     1861 gccctgatgg gcgcgccgcg ccttttgatg ctggacgaac cgagccttgg gctggcgccg
     1921 atcatcgtcg ccgatatttt ccggaccatc ggcgaattgc gcgaaagcgg cgtatcggtg
     1981 ctgctggtcg agcagaacgc gcaggccgcc cttcaaatcg ccgaccgcgc ctatgtgatg
     2041 gagctcggcg aattcattct gaacgggccg gcgaaagaca tcgcgaccaa ccagcgcgtc
     2101 gccgcgagct atctcggctt ccagcacgag ggcgcgagcg ggatttgaaa agtagggtgg
     2161 gcaaaggcgc tcttcgcgcc gtgcccacca cactgatgaa tgaaaacaac aactactgca
     2221 atggtgggca cgcttcgctt tgcctaccgc ctcactcgct cgcggcgatg tatttcccgt
     2281 tcttcaccgt caggatgatg cgcgagcgat cgtcgagacc gtagcggtcc ttttcggtga
     2341 agttgtagac gccctggctc gccgcaatat cccgttcgct caaccaggcc tgacggatgc
     2401 cctcgcgaaa ttccggcgtt cccggcttgc ccgctttcaa cgccaccggg atgacgcgct
     2461 tgagaacttc gaaggcatcg taggaatgtc cggcgaactg gctgcggctg ttggggccat
     2521 acttggcctc ataggcggcg ttgagcgcga ggcccggctt cttggtcagc gcgctgtcgg
     2581 gctggccttc cggattcatc accggccccg acgccatgat gacgccttcc gccgccgcgc
     2641 cggcaatgcg gatgaagtcc atgctggcgg cgccgtgggt ctgatagatc aaacctttgt
     2701 aaccgcgttc gcgaagtgcc gtctgcggca acgccgcggc ggtgccggac gcgccgacaa
     2761 gaaccgcatc gggattggcg gcgaccagct tcagcacctg ccctgcgacc gacgtatccg
     2821 gccgtgcaaa gcgttcttca gcggccagcg tcatgcccat cgggacagcc tgcgtcttga
     2881 agtcgttgaa ccagagatcg ccgtaggaat cggagtagcc gatatagccg acggtcttga
     2941 tgttgtgcgc cttcatatgc tcgtacagca ccttgcccat gatcgggacc ggttgcggca
     3001 tgccgaccga ccacttcaag cgctccggcg tgatcggaaa cggcgccagc ccgaaatgcg
     3061 gaatgccggc ctcgttggcc acggtggaaa ccgcgaccgt tggcggcgtg gtggacgagc
     3121 ccatgatgat gtcggccttg gactcggtga cgaagcgccg cgcgttggtg gtagcggcgg
     3181 tcggatcgcc gccgtcgtcg agcacgatca ccttgaccgg cacgccgcca atttccttcg
     3241 gcacgaattc aagcgcgttg cgctcgggaa tgcccagcgc cgccgcaggc ccggtcgtcg
     3301 tgacggtgat accgatgacg atctcattgg attgcgccag cgcgggcaac cccggcagcc
     3361 ccagcgacgc cgcgataacc gcggcagaca gataaaactt cttcattcag tcctcccttt
     3421 cggtgttact ctaactgtac atcaaaattc gactgataat tcagcccctt ctttaggcga
     3481 tgcgtttctt taggtcatgc gcccggccga gtcagccccg ctggaacctg gcaatgatct
     3541 cttccggtac ggctgccgat ttcagccgcg ccggatcgtc cggatgacga ccgaccagca
     3601 cgcgggtttc gaaggcctcg atcgccaagg cttccccctt gaacaccttg tgggtcacct
     3661 cgaagctcga gcgcttgaaa ctctcgatct ggctttcgat cgtgatccag tcgccatggg
     3721 tcgacgcgat gtagaatttc gcccgcgtgt ccaccatcgg aatgccgacc agaccatatt
     3781 tcttgatgat gtcctgcttg gagaagcccg ccgcctcgaa caggatcgag gtcgagtcgt
     3841 cgaacatctc gaaatagcgc gggtagtaga cgatgttggc ggggtcgcag tcaccccact
     3901 ggatctgcac atccctgcgg ttgacgaaca tgcaatacct cagcgcggcg ccatgcgaag
     3961 cgaggcatcg agccgcacca cctcgccgtt caggtatgga ttgtcgatca tgtgcagcgc
     4021 cagcgaggcg aactcgtcgg cgtgcccaag ccggcgcggg aacggtatcg acgcggcgag
     4081 gctgtcctgc gcctcctgcg gcaaattggc caatagcggc gtca
//
LOCUS       DATNOI010000584        18275 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_109506,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000584 DATNOI010000000
VERSION     DATNOI010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18275
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109506"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VN968_23590"
     CDS             complement(<1..754)
                     /locus_tag="VN968_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXL32250.1"
                     /translation="MYTGKHAHLRPLQPAFIMANSGEAVSYAELEARSNRLAHLFRQR
                     GLKRLDHYAIFMENNSRYLEACGAGERSGLYFTCVNSYLTGSELAYVVNNSESRILIT
                     STAKLAVAREALKECPKVELCIVVDGPSEGDRIVNLLEATRGLPRTPISDEYVGTAML
                     YSSGTTGRPKGILRPLPEQPPTQQLPMFDFLQKLWQYREGMIYLSPAPLYHSAPQAAV
                     NLTIRAGGTAIIMEHFDPERYLELVEKWGVTHS"
     gene            complement(771..1925)
                     /locus_tag="VN968_23595"
     CDS             complement(771..1925)
                     /locus_tag="VN968_23595"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXL32251.1"
                     /translation="MHKGRVVFGAMDEVVFGRPAAEVIVEQMDRLRANRAFLMVSGTL
                     NRQTDEIKKIKKALGVRCAAIFDTMPPHTPREAVIAAAAQARVANADLIVTVGGGSIT
                     DGAKAVQLCLANHVSTVEGIDTIRANKGVAPQLVAPSVRQISVPTTIAGGEFSAIAGV
                     TNQRTKVKEMLRHDLVMPRAAILDPAMTVHTPEWLFLSTGIRAVDHCVEGLCAREAHP
                     YADAQAMKGLSMLAQALPRVKADAKDLDARMDCQIGTWLSMGALSAGIPMGASHGIGY
                     VLGAAFGVPHGYTSCVMLPAVMRWNKPDNGERQELVSAAMGHPGEDAAEVLDWFIRDL
                     GMPRSLHDVKIGPEHFEAIAEQAMATPWIPRNPRKIAGPAQVREILVLAA"
     gene            complement(2112..2366)
                     /locus_tag="VN968_23600"
     CDS             complement(2112..2366)
                     /locus_tag="VN968_23600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32252.1"
                     /translation="MLSVMFTFDEHASEENQNRISNQLLDLPGVHNVGRISPDATKPA
                     LRRMWYAEVADETAASDLVTQLRQHDGIKSADLPAPRGLV"
     gene            complement(2378..5521)
                     /locus_tag="VN968_23605"
     CDS             complement(2378..5521)
                     /locus_tag="VN968_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXL32253.1"
                     /translation="MVDDHGDESETTSLGLLTTAAFTERDLAQGVTRWMIANFQSQLT
                     DAVRSTPFTVPLLCAIACREAGMYWLPLTPHKSAAEILGLAVYDASGDVAGAPRSAFP
                     VNTAQFRLTYGEAFTNLLIDETNKARAARGLSPASMVYKGYGIFQYDLQSVRTDEAFF
                     RSKQWYSFSECVSRAVGELKKKFEATGDIQSAVRAYNGSGPKAEQYARDVMRLLPFCE
                     EAAASQAHPSFAATSSGRAMGVAAYAGSHALSSSDDDPASPADGEVSDTADFETARLL
                     ANIGAPVAPDPLPFAGTTFASGATSGFDIARAQAFLDACMTSHPRVTYGLGKKVPFLG
                     AVPGRDFTQVDCSGFVREAIRLSTNPPAPFPDGSVVQHDWVRARGFEATTIASGEAKD
                     GVVRIAFLRPQDSGDHIGHVVLINGGKTLESHGHVGPDSRLWDGTSWQVKAHVYVLSR
                     NAQLVASQGGATFRAAFAPAPTFAVRHGRRYLATIVLSGFELVAGNDQIAGFLTQRGF
                     TSVTVTGSGGRRQAEGTWSGADTTAQIDPHLADIVELPAALSAPAIIQGGRPDAANIS
                     AKVFSSESLPTHHDGLLVVKIRPESMSPAPAGTLSTMAFAPTTPGLSALSFYERAGMI
                     KRIVPLRKHDESAGPVPRMAAASTLMFAAKPVEASDASAGVSFIELEQGQDAGQLRSA
                     LASDPNVLSVSQVPVRYLAAAPKPRKAPQGGGIGIASTPPDGQVLWNLRQILWQEARG
                     LAGFQDADAVKVAVLDTGVDDQHPDLKIGAYHWQQPDLSRPVSDKDIIGHGTHVSGTI
                     AALINNALGVKGVCKCDLSVWKIFDDEPTYASGQGSFVYYVNPIMYRRALAACVETPV
                     DVINLSIGGSGVPDATEQSLFEQIIAAGTTICAAMGNERQYGSPTSYPAAIPGVIAIG
                     ATGLDDTVTVFSNSGNHISVAAPGKAIWSTLPTYSGQTGFFAVIGSDGRPTQGRPMSR
                     EHNYDAWDGTSMATPHVSGCAALLIAKSNAAGNKLTPEQVRQSLMQSADKVAAMNGAD
                     FSTDYGAGRINLFNLLQ"
     gene            5842..6015
                     /locus_tag="VN968_23610"
     CDS             5842..6015
                     /locus_tag="VN968_23610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32254.1"
                     /translation="MFGLLRRGDATVEQHGDIGDRRRLPEAWHAPWLAACDKQMRDGF
                     NRLDETLGPDVTA"
     gene            complement(6040..6906)
                     /locus_tag="VN968_23615"
     CDS             complement(6040..6906)
                     /locus_tag="VN968_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXL32255.1"
                     /translation="MAQQLRILQSLWAMERRRADQPELPLQTQLTMIRDAGFDGAGVR
                     FIDAAFATEVTDFLRGRGMIWQAQCYPTSVDDLKPVLELVARCGADHVNLQPDVRPRR
                     IEDCIPLLDGWRRLAEQAGVAVHVETHRDRMTTDLFFTLQLLDCFPDLRLTADVSHYL
                     VGREFAWPVDEVNHALIKRILDNSWGIHGRIASREQVQISLGFPQHQGWVELFMGWWE
                     YAIRSWRKRAKPDAVLTFLCELGPPPYAITGPDGAELSDRWEDALKMKDMVRALWQRI
                     VDDDARGTSNAA"
     gene            complement(6910..8262)
                     /locus_tag="VN968_23620"
     CDS             complement(6910..8262)
                     /locus_tag="VN968_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL32256.1"
                     /translation="MTDIIADSTGRSRLRVILAASIGSALEWYDFFLYGTAAALVFGD
                     LFFPKSDPVVGTLLAFLTFGVGFVVRPLGGILFGIMGDRFGRKPVLVATLLMIGIGTT
                     AIGVLPTYAQIGYWAPALLVLLRVIQGLGAGAEYGGAVIFLVENAPAQHRGFWGSFAP
                     LGVSIGNLLAAGAFALVTLLPREELMSWGWRLPFLASFLLILVGIFVRLRIAETPVFT
                     EAVVARGKIERNPAMQALRRHPRNFMVVLGARLAENGLGYLFPVFGLSYVINTLGVPK
                     ADALSALMLAFVVELFAIVGFATLSDRIGRRPVYMFGALAGVAFAFPFFLLVGTREWI
                     WIALAFIVARAVVTAAMFGPQAAYFAELFPPQRRFAGFAFARELGSLLAGGPAPFLAA
                     ALVAWSGSWWPVACYAAGLSALTALAIWAGPETYQESITVDNTPDGAMHAVAAMPLQA
                     "
     gene            complement(8341..9216)
                     /locus_tag="VN968_23625"
     CDS             complement(8341..9216)
                     /locus_tag="VN968_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXL32257.1"
                     /translation="MISEQDVQAYKRDGVIVVPEILGADTLTQLRSVIAELVAGSAKT
                     LEHTDVYDLEPGHTAESPRVRRIKTPHKVHALFDQIVRSPAVLDILKKLIGPGLRLHG
                     SKLNMKSAHYGSPVEWHQDWAFYPHTNDDILAIGVLLDDTDLSNGPMLVTPGTHSGEV
                     WNHHGDDGCFAGLIDPDTVRNEIERAVPCMGRAGSMSFHHVRALHGSAMNTSDRPRNL
                     LLYEVAASDAWPLMGVKDFAEFNSRLLSGDPVVAPRMTNVPVRLPLPPATRQGSIYET
                     QSAAKKSYFTRTRAA"
     gene            9400..10398
                     /locus_tag="VN968_23630"
     CDS             9400..10398
                     /locus_tag="VN968_23630"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXL32258.1"
                     /translation="MSTAGPNESHSAPTLSAVARLAGVSSITVSRVVRLPGLVAPETR
                     GRVEAAMRELGYVPNQVAGSLASARTSSVGVLVPTIANSIFADTVQGLSDELEPLRYA
                     VILAQSRYDSAREDHMLSALLSRRPEAIIMVGSPATDDGGRLLRRAGIPVAETWDLPA
                     DPIDAVAGFDNYAAGAAVGRHLVAQGRQNLAFIGGDDPRATRRWNGFKDAALEAGAKL
                     PRRLILERNASGSVAALANLPGVDAVFAANDAHAIGFMSGLRAAGLLRNGPASEQPVA
                     VIGLGDLEMGRLIAPSLSTIRVHGDAIGRTAAKLMLTREGPRHVDLGFELVLRDSG"
     gene            complement(10456..12237)
                     /locus_tag="VN968_23635"
     CDS             complement(10456..12237)
                     /locus_tag="VN968_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL32259.1"
                     /translation="MDALPSQKSFAEAHNPDRGADRIAPDCAGQNFYATDRGLRDLLK
                     LYLEPDDFRRLEPHFHHLGALAGGRLDELARAADKHPPVLNARDRFGRDEDRIDYHSS
                     YREMEKIAFGDFQFHAMSHRAGTLGMDRPLPAVAKYALQYLFVQAEFGLMCPISVTDT
                     SIHLIRKFASAELQDYLLPKMLSGDVATMWKGTQFVTERSGGSDVGAIETTARCEDEV
                     WRLFGDKWFCSHADADVALLLARPEGAAPGTRGLALFALPRRLKDGRRNAYRIVRLKD
                     KLGTRSMASGEIVLEGAVAYLVGEADRGLKQMMEQVNLSRLSHGVRAAAMMRRGVNEA
                     MVCARGRLAFGKAIIEFPLLRRQLLKITVPTEQALSMFLFAADAMDRANAGSKQAESV
                     LRILTPLLKFRACRDNILVATGSMEIRGGNGYIEEWVNARLVRDAHIGVLWEGTSNIN
                     ALDIITRAVGKSRAHRTLEAALRKLLDEATPIPSAFRDRLRLALDRTLGFAERVAAEA
                     ALGATARQAASALYHVTSAILMTWEAAQPAADARRALYARFVLEHRLSAQDPLEPRTG
                     DWERDAAQIVFSDRTVSLPEIAGLLER"
     gene            12717..13886
                     /locus_tag="VN968_23640"
     CDS             12717..13886
                     /locus_tag="VN968_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXL32260.1"
                     /translation="MFNFHSFCDLRPLVVLLAVSLSGCGDKPPPQAAAGPPPVTVAQP
                     TKRTVTDWDEFTGRFEAVEEVQVRARVGGFVTNVEFRDGAFVKTGDLLYIIDARPFEA
                     VAEQADGQLSDARAKAELAKRELDRALTLNQTQAVSDSIVDQRRQALQAAKASEMQAA
                     GALKAAQLNIEFTHVIAPIGGRVSRHLISIGNLVQGSDAGGGTLLTSIVSLDPIYVYF
                     DMDEATYLKNNRLYFEGKRPSSRENPNPVQVSLTGETKPSHDGKVDFLDNRLDLSTGT
                     LRGRAVIPNKDFSILPGQFGRVRLIGSAPYEALLLPDTAIATDQSRKIVFVVKDDDTV
                     EARPVVLGPLDEGLRVIREGLKAEDRVIVDGLQRARVGAKVSPHAAPAAPAGSKT"
     gene            13883..17041
                     /locus_tag="VN968_23645"
     CDS             13883..17041
                     /locus_tag="VN968_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HXL32261.1"
                     /translation="MNLGQLSINRPILAMVLSIVLLIVGAIAYTTLPVAEYPQVVPPT
                     VVVTTQYPGASAQTVSDTVAAPIEQEINGVEDMLYLYSQATSNGNLTITVTFKLGTDL
                     DKAQVLVQNRVAIAQPRLPEEVQRNGVVTRKNSPDILMVVFMLSPDDSFDQLYISNYA
                     LLQVRDQLLRLDGIGDIQMFGARDYSMRLWLDPDRIATLGMTAGEVLAAIRAQNLQIT
                     GGQLAEPPIADRAFQPNLTFTGRLKDIGQFEDIVVKAGGDGRTVRLRDVARIELGALS
                     YSTNSFLLRKSAVALLVTQRPGSNALATAKNISNAMVKLKADFPRGLDYNIGYNPTEF
                     IAQSVSELIKTIYEAMLLVVIVVLVFLQGWRPAIIPIVAIPVSLVGTFAVMAALGFSI
                     NNLTLFGLVLAVGIVVDDAIVVVENVERHLRDGMSRRDAALKTMEEVGGALVSIALVL
                     CAVFVPTAFLGGISGQFFQQFAITIAVATAISCFCSLTLSPALASQILQPHEDKRPPA
                     SWNFIGRGWDKFTGFFNRGFDRLSHGYGNAAGFVIRHSVVMLLVYVVLIGSAGWLIVT
                     TPQGFIPAQDRGYVIISAQLPGAASLERTTEVVREIERIALDTPGIVRVAVFAGFSGA
                     TRTQAGNAAALFPVFEEPDVRLKKGLSAAVITGELRKRLAALQGAFIIVIPPPAVPGI
                     GTGGGFTMRIQDRQGRGPELLAAATDELVAAARKAPGLTSVFSPFTANTPQVFVDIDR
                     IKAQKLGVPIQNVTETIQTYFGSAYVNDFNLFGRTYHVTAQADLPFRKETTDLARLRT
                     RNAAGDMVLLGNVVNFRDISGPDRVARYNLYPASELQGDTTPGTSSATAIDTMKRLAA
                     DTLPSGFAYEWTDLSYQQVTGGNSGLYVFPICVLFVFLVLAAQYGSWSLPFAVLLIVP
                     MCLLAATIGVRIMGQDVNILTQIGFVVLVGLAAKNAILIVEFARDIELEGRERLAAVI
                     EACRLRLRPILMTSFAFILGVLPLVVSTGSGSEMRQAVGVAVFFGMLGVTLFGLIFTP
                     IFYVMVRNLADRRHDKKAVAGG"
     gene            17167..>18275
                     /locus_tag="VN968_23650"
     CDS             17167..>18275
                     /locus_tag="VN968_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32262.1"
                     /translation="MKSGLLAAVAAGGLLLAGPASAQGVKIGILNDQSGVYADYGGKY
                     SVEAAKMAVEEFGGSVLGQKIEVVSADHQNKADLATAIARRWYEVEGVDMITELTTSS
                     VALAIHDLSKQMKKIDIVVGAATSRLTGDACQPYGFHWAYDTHALAVGTGGALVEAGG
                     DTWFFMTADYAFGYALEKDTGDLVKEKGGKVLGAVRIPLNSSDFSSFLLQAQSSKAKI
                     IGLANAGLDTTNSIKQAAEFGIVKGGQKLAGLLLTLAEVHGLGLEAAQGLVLTEGYYW
                     DRDDKSRDLAKRFFARTGRMPNMIQAGTYSATLQYLKAVKAAGTKDTEAVAQKLKELP
                     VDDLFAQGGKVLENGRMVHDLYLFEVKKPSESKKP"
BASE COUNT         3259 a         5773 c         5826 g         3417 t
ORIGIN      
        1 ggctgtgggt gacgccccat ttctcgacca gctccagata acgctcgggg tcgaagtgtt
       61 ccatgatgat ggcggtgccg ccggcacgga tcgtgagatt gaccgcggcc tgcggcgccg
      121 aatgatacag cggcgcgggc gacaggtaga tcatgccctc gcggtactgc cagagttttt
      181 gcaggaagtc gaacatcggc agctgttggg tcggcggctg ctccggcagc ggccgcagaa
      241 tgcccttcgg ccggccggtg gtgccggacg aatagagcat cgcggtgccg acgtattcgt
      301 ccgatattgg cgtcctgggc aggccgcgcg tcgcctccag cagattgacg atgcggtcgc
      361 cttcgctggg accatcgacg acgatgcata gctcgacctt ggggcattcc ttcagcgcct
      421 cgcgggcgac ggcgagcttt gccgtggacg tgatgaggat gcgcgactcg ctgttattga
      481 cgacgtaagc gagctcgctt ccggtcaaat atgaattcac gcacgtaaag tataatccgc
      541 tccgctcgcc ggcgccgcag gcttcgagat agcggctgtt gttctccatg aagatcgcgt
      601 aatggtcgag ccgcttcagg ccgcgttggc gaaacagatg cgccagacgg ttgctgcgcg
      661 cctcgagttc ggcatagctc acggcttcgc cggagttcgc catgatgaaa gcgggctgaa
      721 gcgggcggag atgagcatgc ttgccggtgt acatcgtgtc tccaatgttc tcatgcggcc
      781 agaacaagaa tctcgcgcac ttgtgccggg ccagcgatct tgcgcgggtt gcgcggaatc
      841 catggcgtcg ccatcgcctg ctccgcgatc gcctcgaaat gctccgggcc gattttgacg
      901 tcatgcaggc tgcgcggcat tccgaggtcg cggatgaacc agtccagcac ctcagcagcg
      961 tcctcgccgg gatgtcccat cgcagcggat accagttcct gccgctcgcc attgtcaggc
     1021 ttgttccagc gcatcaccgc cggcagcatc acgcaggagg tatatccgtg cggcacgccg
     1081 aacgccgcgc caagcacata accgatgccg tggctggcgc ccatcgggat gccggccgac
     1141 aacgctccca tcgagagcca ggtgccgatc tggcagtcca tcctggcgtc gagatccttc
     1201 gcgtcggcct tcacgcgcgg cagcgcctgc gccagcatcg acagtccctt catcgcttgc
     1261 gcgtcggcgt agggatgggc ctcccgcgcg cacaggcctt cgacacaatg gtcgacggcg
     1321 cggatgccgg tcgacagaaa cagccattcc ggcgtgtgca cggtcatcgc gggatcgagg
     1381 atcgccgcgc gcggcatcac gaggtcatgg cgcagcatct ccttgacctt ggtgcgctgg
     1441 ttggtgacgc cggcgatggc gctgaactct ccgccggcaa tcgtcgtcgg cacgctgatc
     1501 tggcgtaccg acggtgcgac cagctgcggc gcgacgccct tgttggcgcg gatcgtatcg
     1561 atgccctcga cggtgctgac atggttggcg aggcaaagct gcaccgcctt ggcgccgtcg
     1621 gtgatcgagc cgccgcccac cgtgacgatg agatcggcgt tggcgacccg ggcctgcgcg
     1681 gcggcagcga tcaccgcctc gcgcggcgtg tgcggcggca tcgtgtcgaa aatggccgcg
     1741 caacggaccc cgagggcttt tttaattttc ttgatttcat cggtttgccg gttgagcgta
     1801 ccgctcacca tcaggaacgc gcggttggcg cgcaggcgat ccatctgttc gactataacc
     1861 tcggcagccg ggcgtccgaa cacgacctcg tccatggcgc cgaacacgac acgtcccttg
     1921 tgcacgattg tcctcccagg acgattttgt tttggtgtcg ccttggcagc aatctagcgg
     1981 agtgaacgag ggcaggggaa tcaaaaaaat ggtgttggtc ctccgccgcc gctatgcgtc
     2041 tccggcgcgg tgacgcccgg gcggagacgc gaactcacct agctgtgctg cggtgtgtcg
     2101 ctgatctacc gctagaccag cccgcgcggg gcgggcaaat cggcggactt gattccatca
     2161 tgctggcgca actgggtcac caaatcggag gccgccgtct cgtcggcgac ctcggcgtac
     2221 cacattcgtc gcagcgccgg cttggtggcg tcaggactta tacgtccgac attgtgcacg
     2281 ccggggagat ccaggagctg attgctgatc cggttctggt tctcctccga cgcgtgctca
     2341 tcgaacgtga acatcacgct aagcatgctt tagtcaccta ttgcagcagg ttgaacagat
     2401 tgatccggcc ggcgccgtaa tcggtgctga aatctgctcc gttcatcgcg gctaccttgt
     2461 cggccgattg catcaacgac tgtcttactt gttcgggcgt cagtttgttc cctgcggcat
     2521 tgctttttgc gatcaacaag gccgcacacc cgctgacatg gggtgtcgcc atcgaagttc
     2581 catcccaggc gtcgtaatta tgttcgcggc tcatgggcct tccctgtgtc ggtcttccgt
     2641 cggagccgat cacggcaaag aacccggtct gtccggaata ggtgggcaat gtggaccaga
     2701 tcgccttacc gggagcggca accgagatgt ggttaccgct gttggaaaat accgtcaccg
     2761 tgtcatcgag gccggtcgcc ccgatcgcaa tcacgcccgg aatggcggca ggataagagg
     2821 tcgggctgcc atattggcgc tcgttgccca ttgccgcgca gatcgtcgtt cccgcggcaa
     2881 ttatctgctc gaacagcgat tgttcggttg catcaggaac gcccgaacct ccgatgctga
     2941 gattgatcac gtcgaccggc gtttccacgc aggcggcgag agctcggcgg tacatgatcg
     3001 ggttgacata atagacgaac gatccttggc ccgacgcgta ggtcggctcg tcatcgaaga
     3061 tcttccagac gctgaggtcg cacttgcaaa ctcctttaac gccgagggca ttgttgatca
     3121 acgcagcgat cgtgccggac acatgggtac cgtggccgat gatgtccttg tcggagaccg
     3181 gccgcgatag gtcgggctgc tgccaatggt aggcgccgat cttgagatca gggtgttgat
     3241 cgtcgacgcc cgtatcgaga accgccacct tgacggcgtc tgcgtcctgg aagccggcca
     3301 ggccgcgcgc ttcctgccag aggatctgcc tgagattcca gaggacctgt ccgtccggcg
     3361 gcgttgacgc gatcccgatg cctcctccct gaggggcctt gcgcggcttc ggcgcggctg
     3421 cgagataccg caccgggacc tgcgacaccg agagcacgtt gggatcgctt gccagcgccg
     3481 agcgcagctg cccggcatcc tggccctgct caagttcgat gaagcttacc ccggcgctgg
     3541 cgtcagacgc ttcgacgggc ttcgccgcga acatcaaggt ggatgcggca gccatccgtg
     3601 gcactggccc ggcactttca tcgtgcttgc gcaggggcac tatccgcttg atcatgccgg
     3661 cgcgctcgta gaacgacagc gcgctcaggc ctggcgttgt cggcgcgaaa gccatggtgc
     3721 tgagggttcc cgcaggggcg ggcgacatcg attcggggcg gattttgacg accagcaatc
     3781 cgtcgtggtg cgtgggaagc gactccgagc tgaacacttt cgcagagata ttggccgcgt
     3841 ccggccttcc accctggatg atcgccggcg cggacagggc cgccggtaat tcgacgatgt
     3901 cggcaaggtg cggatcgatc tgagcggtcg tatcggcccc gctccaggtt ccttccgcct
     3961 gccgccggcc accgcttccc gtgacggtta cactggtgaa gccccgctgc gtgagaaagc
     4021 cggcgatctg gtcgttgccg gcgacgagtt cgaatccgga aagcacgatg gtggccaagt
     4081 agcgccggcc atggcggacg gcgaaggtcg gagccggagc gaaggccgcg cggaacgtcg
     4141 cgccgccctg cgatgcgaca agttgcgcgt tgcgcgacag cacatagacg tgtgccttga
     4201 cctgccagct ggtgccgtcc cataagcgcg agtccggccc gacgtggcca tgcgactcca
     4261 gggtcttgcc gccgttgatc agaacgacat ggccgatatg gtcgcccgag tcctgtggcc
     4321 ggagaaacgc aatgcggacc acgccgtcct tggcctcgcc cgacgcgatg gtcgtggcct
     4381 cgaacccgcg cgcgcgaacc cagtcgtgct ggacgaccga gccatccggg aacggagccg
     4441 gaggatttgt cgaaagacga atcgcttcac gcacaaaacc gctgcagtcc acttgcgtga
     4501 aatcgcgccc gggcacggct ccgaggaaag ggaccttttt gccgagcccg taggtcacgc
     4561 gcggatgcga tgtcatgcac gcatcgagga aggcctgggc gcgcgcgata tcaaagccag
     4621 acgtcgcgcc agacgcgaag gtcgtgccgg cgaaaggcag cggatccggc gccaccggcg
     4681 caccgatatt ggcgagcagc cgcgccgttt cgaagtcggc ggtgtcgctg acctccccat
     4741 cggccggact ggccgggtca tcatcgcttg acgaaagcgc gtggcttcca gcgtatgccg
     4801 caacacccat agcgcgaccc gacgaggtcg ccgcaaagct cggatgcgcc tggctcgccg
     4861 cagcctcctc gcagaacggg agcagccgca tgacgtcgcg cgcgtattgt tcggcctttg
     4921 ggccggatcc attgtaggca cgaacggcgc tttgaatgtc gccggtggct tcgaacttct
     4981 tcttcagttc gccgacagcg cggctaacgc attccgagaa actgtaccat tgcttcgatc
     5041 ggaagaaggc ctcatcggtc cggacggatt ggaggtcgta ttggaagatg ccatagccct
     5101 tgtagaccat cgacgcagga ctgagaccgc gcgcggcgcg agctttgttg gtttcgtcaa
     5161 tcagcaagtt ggtgaacgct tcgccatagg taaggcggaa ttgcgcggtg ttgaccggga
     5221 acgcgctccg cggggcgccc gcgacgtcgc cgctggcatc gtagacggcg agcccgagga
     5281 tttccgctgc tgatttgtgc ggcgtaagcg ggagccagta catgccggct tccctgcagg
     5341 cgatcgcgca gagcagtggc acggtgaacg gcgtcgatct gacggcatcg gtgagttgcg
     5401 actggaaatt ggcgatcatc cagcgcgtga cgccctgcgc cagatcgcgc tcggtgaacg
     5461 ccgccgtcgt cagcaagccg aggcttgtcg tttcgctttc gtcaccatga tcgtcaacca
     5521 tcgccgtttt cctccgtgat gcatttttgg tctgccggtt gctcgacgct cgccatcgcc
     5581 gctatcctct caatcgcggt gcctcgcttt gccggtgcct ctccccgacc attgcgggcc
     5641 aagctagcga agcttgactg tgcgcagccc tgccgcgacg acttcggtgg ccgctcccgc
     5701 gaggaacagc gcggcgcaat cgacggggct cccgccccgt tgcgctctac tcgcaacttc
     5761 agagatgttg cgatgattta tgctaccgag ggacgacgcg cacatatgaa gtaaatcaca
     5821 aatgccgttc cggcttcgct gttgtttggt ctgctgcggc gcggtgatgc aaccgtcgag
     5881 cagcacggcg atatcggcga ccgccgccgg ctacccgagg catggcatgc gccatggctg
     5941 gcggcatgcg acaagcagat gcgcgatggg ttcaaccggc tcgatgagac tctcgggccc
     6001 gacgtgacgg cgtagcaaaa cgacggtctg cgcgtagtct cacgccgcat tgctcgtgcc
     6061 gcgcgcgtcg tcgtcaacga tgcgctgcca cagcgcccgg accatgtctt tcattttcag
     6121 cgcgtcttcc cagcggtccg atagctcggc gccgtcaggc ccggtgatgg cgtagggcgg
     6181 cgggccgagt tcgcacagga aggtcagcac ggcgtcgggt ttcgcgcgtt tccgccagga
     6241 gcggatggcg tattcccacc atcccatgaa caattcgacc caaccctggt gctgcggaaa
     6301 gccgagcgag atctgcacct gctcgcggct ggcgatccgg ccatgaatgc cccagctgtt
     6361 gtcgagaatg cgtttgatca gggcgtgatt gacctcgtcg accggccagg cgaattcgcg
     6421 gcccaccagg taatgcgaaa cgtccgccgt cagccgcagg tcgggaaagc aatccagcag
     6481 ctgcagtgtg aaaaaaagat ccgtggtcat gcggtcgcgg tgggtttcga catgtaccgc
     6541 gacaccggcc tgctcggcca gccgacgcca tccgtcaagc aggggaatgc aatcctcgat
     6601 ccgccgcggc cgcacgtcag gctgcaggtt gacgtggtcg gccccacacc gcgccaccag
     6661 ttccagtacc ggtttgaggt catcgacgct ggtcggatag cattgcgcct gccatatcat
     6721 tccgcgcccg cgcaggaagt cagtgacctc ggtggcgaaa gcagcatcga tgaagcggac
     6781 gccggcgcca tcgaaaccgg cgtcgcggat catcgtgagc tgggtttgca gcggcaactc
     6841 gggttgatcc gcccgccgcc gctccatcgc ccagagcgat tgcaggatgc gaagttgctg
     6901 cgccacggtc tacgcttgca gtggcatggc cgccaccgcg tgcatcgcgc catccggcgt
     6961 attatcgacc gtgatgcttt cctgatacgt ctcggggccg gcccagatcg ccagcgccgt
     7021 gagtgcggac agaccggcgg catagcaggc gaccggccac cagcttcccg accatgccac
     7081 cagcgccgcg gccaggaacg gcgccggacc gccggccagc agcgagccca gttcgcgggc
     7141 gaaggcgaag ccggcaaagc gccgctgcgg cggaaacaac tcggcgaaat aggcggcctg
     7201 cgggccgaac atcgccgccg tcaccaccgc ccgcgccacg atgaaggcca gcgcgatcca
     7261 gatccattcc cttgtgccga ccagcaggaa gaacgggaac gcgaaggcca cgccggccag
     7321 cgcgccgaac atatagactg ggcgccgtcc gatccggtcg gagagcgtgg cgaaaccgac
     7381 gatggcgaac agttcgacca cgaaggcgag catcagcgcg ctcagggcat cggcttttgg
     7441 cacgcccagc gtgttgatga cgtagctcag gccgaacacc ggaaacagat agccgaggcc
     7501 gttttcggcg agccgcgcgc cgagcaccac catgaaatta cgcggatgcc ggcgcagcgc
     7561 ttgcatcgct ggattgcgct cgatcttgcc acgcgccacc accgcctcgg tgaaaacagg
     7621 cgtttccgcg atgcggagac gaacgaaaat gccgacgagg ataagcagga agcttgccag
     7681 gaacggtaga cgccagcccc acgacatcag ctcttcgcgc ggcaatagcg tcaccagcgc
     7741 gaaagcaccg gctgcgagca gattgccgat cgacacgccg agcggtgcga agctgcccca
     7801 gaaaccgcga tgctgtgccg gggcgttctc caccaggaag atcaccgcgc cgccatattc
     7861 ggcgccggct cccaatcctt gaatgacacg cagcaagacc aatagcgccg gcgcccaata
     7921 gccgatctgg gcataggtcg gcaacacgcc gatggcggtg gtgccgatcc cgatcatcag
     7981 cagtgtcgcc accagcaccg gcttgcgccc gaaccggtcg cccatgatgc cgaacaggat
     8041 gccgccaagc ggtcgcacca cgaagccgac gccgaaggtc aggaacgcca gcagcgtgcc
     8101 gaccaccggg tcgctcttcg gaaagaacag atccccgaac accagcgccg cggcggtgcc
     8161 gtagaggaag aagtcatacc attcgagcgc ggatccgatg ctggctgcaa ggatcacgcg
     8221 caggcgcgaa cgtccggtgc tatcggctat gatgtccgtc atcgtttccc ccgttttgtt
     8281 tttgtcatct tattcgttgg tagtcggcag ctgcgccttg gcgcggccgc cgtcgcaaga
     8341 tcacgcagcg cgggtgcggg tgaagtagga tttcttcgcc gcggattgcg tttcgtaaat
     8401 cgatccctgc cgcgtcgcag ggggcagcgg aaggcgcacg ggcacgttgg tcatccgcgg
     8461 cgccaccacg gggtcgcccg acagcaaccg gctgttgaat tcggcaaaat ccttcacgcc
     8521 catcagcggc caggcgtcgc tcgccgccac ctcgtacagc aacaggttgc gcggccggtc
     8581 cgacgtgttc atggccgatc cgtgcaacgc ccggacatgg tggaacgaca tgctgccggc
     8641 gcgtcccatg caaggcaccg cgcgttcgat ctcgtttcgg acggtgtcgg gatcgatcaa
     8701 tccggcaaaa cagccgtcat cgccatggtg attccagacc tcgccgctat gcgtgcccgg
     8761 cgtgaccagc atcggtccgt tcgacagatc ggtatcgtcg agcagcacgc cgatcgcgag
     8821 aatgtcgtca ttggtgtgcg gatagaacgc ccagtcctga tgccattcca ccggcgatcc
     8881 gtaatgcgcc gacttcatgt tgagttttga gccgtgcagc ctcaagcccg gtccgatcag
     8941 ctttttgagg atgtcgagca cggccggcga ccggacgatc tggtcgaaca gcgcatgcac
     9001 cttgtgcggg gtcttgatcc ggcggacgcg ggggctttcc gcggtgtgac ccggttcgag
     9061 gtcatagaca tcggtgtgtt cgagagtttt tgccgacccc gcaaccagtt ccgcgatcac
     9121 cgagcgcagc tgggtgaggg tatccgcgcc cagtatttca ggcaccacga tcaccccatc
     9181 gcgcttgtag gcctgcacat cctgctctga aatcatccag cttctctcct tattgccttc
     9241 cgacccgctt tgcgacgcgg gttctctcgc cagcgggctc gccatgatag cgctatcatt
     9301 ctgatggaaa cggcgaccgc accagaatga tagcgctgtc atttctcttg tgcaagccga
     9361 aatcatgtat tccggcgggg aattcggtag tctcttggca tgagcaccgc cggaccgaac
     9421 gagtcgcatt ccgccccaac cctgtccgcc gtggcgaggc ttgcgggcgt atcgtccatc
     9481 accgtcagcc gcgtggtgcg tttgccgggc cttgtggcgc cggagacccg gggccgggtc
     9541 gaggccgcga tgcgggaact gggttatgtg ccgaaccagg tggcaggctc cctggcaagt
     9601 gcccgtacca gctcggtcgg cgtgctggtg ccgaccatcg ccaattcgat cttcgccgat
     9661 accgtgcagg gcctgtccga tgaattggag ccgctccgct atgccgtgat cctggcgcag
     9721 tcgcgttacg actcggcaag ggaagatcat atgctttcag cgctgctgtc gcggcgcccc
     9781 gaggcgatca tcatggtcgg ctcgcccgcg accgacgacg gcggccggct gctgcggcgc
     9841 gccggcattc ccgtcgccga gacctgggat cttccggctg atccgatcga cgccgtcgcc
     9901 ggtttcgaca attacgccgc tggggccgcg gtcgggcggc atctggttgc gcaaggtcgc
     9961 caaaatctgg cgttcatcgg cggcgacgat ccccgggcca cgcgccgctg gaatggattc
    10021 aaggacgcag ccctggaagc cggtgcgaaa cttccgcgcc gattgattct ggagcgcaac
    10081 gcctcgggca gtgttgccgc gctggccaac ctgcccggtg tggacgcggt gttcgccgcc
    10141 aatgatgcgc atgcgatcgg cttcatgtcc ggcctgcgcg ccgccgggtt gttacgaaat
    10201 ggtccagcct cagagcagcc cgtcgccgtc attggtctgg gcgatctgga aatgggccgg
    10261 ctgatcgcgc ccagtctgag caccatccgc gtgcatggcg acgccatcgg ccgcaccgcc
    10321 gcaaagctga tgctgacgcg cgagggaccg cgtcatgtcg atctcggctt tgaactggtg
    10381 ctgcgcgaca gcgggtgaga tgcggtttcc ccgtcattcc gggttcgacg ctgcgcgtcg
    10441 ccccggaatg acggactatc gctcaagcag cccggcgatt tcgggcagcg aaaccgtccg
    10501 atccgaaaat acaatctgcg ccgcatcgcg ctcccagtcg cccgtgcgag gctccagcgg
    10561 atcttgcgcg ctcaggcgat gttcgagcac aaacctcgca tacagcgccc ggcgcgcgtc
    10621 cgccgccggc tgggctgctt cccatgtcat gagtatggcg ctggtgacgt gatagagcgc
    10681 acttgcagcc tgccgcgcgg tggcgcccag cgcggcctcg gctgcgacgc gctcggcgaa
    10741 cccaagcgtc cggtcgagcg caaggcgcag gcgatcgcga aaagccgacg gaatcggcgt
    10801 ggcctcgtcc agcaatttcc tgagcgcggc ttccagcgtc cggtgggcgc ggctcttgcc
    10861 gaccgcgcgg gtgatgatat cgagcgcgtt gatgttgctg gtgccttccc agagcacgcc
    10921 gatatgggcg tcgcgaacca gtcgcgcatt cacccactcc tcgatgtaac cgttgccgcc
    10981 gcggatttcc atcgaacccg tcgccaccag aatgttgtcg cggcaggcgc ggaatttgag
    11041 cagcggggtg agaattcgca aaacactttc cgcttgcttc gaaccggcat tggcgcggtc
    11101 catcgcatca gcggcaaaca ggaacatcga gagcgcctgt tcggtcggca cggtgatctt
    11161 caagagctgg cggcggagca ggggaaattc gataatggcc ttgccgaagg cgaggcgacc
    11221 gcgcgcgcaa accatcgcct cgttgacgcc gcgccgcatc atcgcagcag cccgaacgcc
    11281 gtgcgaaagc cgcgacaggt tgacctgctc catcatctgc ttgaggccgc ggtcggcctc
    11341 gccgacgaga taggcgaccg cgccctccaa aacgatctcg ccgctcgcca tcgagcgggt
    11401 gcccagcttg tccttgaggc gaacgatgcg ataggcgttg cggcggccgt ccttcagccg
    11461 gcgcggcagg gcgaacaacg ccagtcccct ggtaccgggg gcggcacctt ccgggcgcgc
    11521 cagcagcagt gctacatccg cgtcggcgtg cgagcagaac catttgtcgc cgaacagccg
    11581 ccacacctcg tcctcgcagc gcgcggtggt ttcgatcgcg ccgacgtcgg agccgccgga
    11641 acgttcggtc acgaactgcg tgcccttcca catcgtcgcg acatcgcctg acagcatttt
    11701 cggcagcaga tagtcttgga gctcggcgct ggcgaatttg cgaatgagat ggatcgaggt
    11761 gtcggtcacg ctgatcggac acatcagccc gaactcggcc tgcacgaaca gatattgcag
    11821 cgcatatttg gctaccgcgg gcagcgggcg atccatccca agcgtgccgg cgcgatggct
    11881 catggcgtga aactggaaat cgccgaacgc gattttctcc atttcgcggt aggacgaatg
    11941 atagtcgatc cggtcctcgt cgcggccgaa ccgatcgcgt gcgttcaaga ccggcgggtg
    12001 cttgtcggcg gccctcgcca gttcgtcgag cctgccgccc gccagcgcac cgaggtgatg
    12061 gaaatgcggt tccagccggc gaaaatcatc tggctcaaga tacagcttta gaagatcgcg
    12121 caggccgcga tccgttgcat aaaaattctg cccggcgcaa tccggcgcga tgcgatcggc
    12181 gccgcgatcg ggattgtgcg cttcggcgaa cgatttttgc gaaggaagcg cgtccatgtc
    12241 tgaccctcgt tttgcggatc ggcggatcga cgctcaatat cgttttccag cggaatattc
    12301 caacactatt ggtgcagcgg cggcggattg tgaaattcca caaccgggtt gaccgggccg
    12361 tatggatcgt acgaattgat tgagcccgga aatgtcgagc aatttcccaa tctctgaaca
    12421 tcggattgcg atcaacgccg caccggaaca cgtggacgcc gcctagccat ctggccgggc
    12481 ttttcggcta acggaccgtc acactagcgc cgatgatgcc gaataccttc atgacgctcg
    12541 cccgcgcgcg caacgcatga taattttgct tgatcccggc cgggtcttga acagactggc
    12601 gaccggtctc atcttggtcc tgccgcggat catgacggcc gccttgatgg tggcaaagtg
    12661 tggaggggat ggaattggcc gcgactgaga aatcggagcg tttagctcac atagaaattt
    12721 tcaatttcca ttcgttctgc gacctcagac cgctggtcgt tctgctcgcg gtgtccctgt
    12781 ccggctgcgg ggacaagccg ccgccgcagg ccgcggcagg tccgccgccg gtcacggtcg
    12841 cgcagcccac caaacgtacc gtcaccgatt gggacgaatt caccggacgg ttcgaggcgg
    12901 ttgaagaggt ccaggttcgc gcccgggtcg gcggttttgt caccaatgtc gagtttcgtg
    12961 acggcgcctt cgtcaaaacc ggcgatctcc tgtacatcat cgacgcccgc cccttcgagg
    13021 ccgtcgccga gcaggccgac ggccagcttt cggatgcccg cgccaaggcg gaactggcaa
    13081 aacgtgaact cgaccgtgcg ctcaccttga atcaaaccca ggcggtctcc gattccattg
    13141 tcgatcagcg ccgccaggcc ctgcaggccg caaaggcctc ggagatgcag gccgccggcg
    13201 cgctgaaggc agcccagctc aacatcgaat tcacccacgt catagccccg atcggcggca
    13261 gggtgagccg ccatctcatc agcatcggca atctcgtgca gggcagcgac gccggcggcg
    13321 gcacgcttct gacctcgatc gtctcgctcg atccgatcta cgtttacttc gacatggacg
    13381 aggcgaccta tctcaagaac aaccggcttt atttcgaggg caagcgtccg agttcgcgcg
    13441 agaacccaaa cccggtccag gtcagtctga ccggcgaaac caagccgtca catgacggca
    13501 aggttgattt cctcgacaac cggctggatc tctcgaccgg cacgctgcgc gggcgggcgg
    13561 tgatcccgaa caaggacttt tcgattctgc cggggcagtt cggccgggtg cggctgatcg
    13621 gaagcgcgcc ttacgaagcg ctgctgctgc cggataccgc catcgccacc gaccaatcgc
    13681 gcaagatcgt gttcgtggtc aaggacgacg atacggtgga ggcaagaccc gttgtcctcg
    13741 gacccctcga cgagggcttg cgcgtgatcc gcgagggcct gaaggcggaa gaccgcgtga
    13801 ttgtcgatgg cctgcagcgt gcgcgcgtcg gcgccaaggt gagcccgcac gcagcgcccg
    13861 cagctcctgc cggtagcaag acatgaacct cggtcagctt tccatcaatc ggccgatcct
    13921 ggcgatggtg ctgtccatcg tgctgctgat cgtcggcgcc atcgcctaca ccacgctgcc
    13981 ggtcgcggaa tatccgcaag tggtgccgcc aaccgtcgtc gtcaccacgc aatatcccgg
    14041 cgcttccgcg caaacggttt ccgacacggt cgcagccccg atcgagcagg agatcaatgg
    14101 cgtcgaggac atgctgtacc tctatagcca ggcgacctcg aacggcaatc tcaccatcac
    14161 ggtcaccttc aagcttggca ccgacctcga caaggcccag gtgctggttc agaaccgcgt
    14221 cgcgatcgcg cagccacggc tgccggagga ggtgcagcgc aatggcgtcg tcacgcgcaa
    14281 aaacagcccc gacatcctga tggtcgtatt catgctgtcg cccgacgaca gcttcgacca
    14341 gctctacatc agcaattatg cgctgctgca ggtgcgcgac cagttgctgc ggctcgatgg
    14401 catcggcgat atccagatgt tcggcgcgcg cgactactcg atgcggctgt ggcttgatcc
    14461 ggatcggatc gcgacgctgg gcatgaccgc gggcgaagtg ctggcggcga tccgcgccca
    14521 gaacctgcag atcaccggcg gccagctcgc cgagccgccg atcgccgacc gtgccttcca
    14581 gccgaacctc acctttaccg gccgcctgaa ggatatcggg cagttcgagg atatcgtggt
    14641 caaggcaggt ggcgacggcc gcaccgtgcg cttgcgtgat gtggcgcgga tcgagctggg
    14701 cgccttgtca tactccacca acagcttcct gctcaggaaa tcagcggtcg cgctattggt
    14761 gacccagcgt cccggatcga atgcgctcgc cacggcgaaa aacatctcca acgccatggt
    14821 caaactcaag gccgatttcc cgcgcgggct cgattacaat atcggctaca atccgaccga
    14881 gttcatcgcg caatccgtca gcgagctgat caagacgatc tacgaggcga tgttgcttgt
    14941 cgtcatcgtc gtgctggtgt tcctgcaggg ctggcggcct gcgatcatcc cgattgtcgc
    15001 cataccggtg tcgctggtcg gcaccttcgc cgtgatggcg gcgctcggat tttccatcaa
    15061 caatctgacc ctgtttggcc tcgtgctggc ggtcggaatc gtggtcgacg acgcgatcgt
    15121 ggtggtcgaa aatgtcgagc gccatcttcg cgatggcatg agccggcgcg atgcggcgct
    15181 caagacgatg gaggaagtcg gcggcgcgct ggtctcgatc gcgctggtac tgtgtgcggt
    15241 gttcgtgccg accgcgtttc tgggcggcat ttccggccag ttctttcagc agttcgcgat
    15301 cacgatcgcg gtagccacgg cgatttcctg cttttgttcg ctgacgcttt cgcctgcgct
    15361 ggcgtcgcag atcctgcagc cgcatgaaga caagcggccg cccgcaagct ggaatttcat
    15421 cgggcgcggg tgggacaaat tcaccggctt cttcaatcgc ggttttgatc ggctgtccca
    15481 cggttacggc aacgcggcgg gctttgtgat ccggcattcg gtcgtcatgt tgctggttta
    15541 cgttgtgctg atcggcagcg ccggatggct gatcgtcacc acgccgcagg gctttattcc
    15601 ggcgcaggat cgcggctatg tcatcatttc cgcgcaattg ccgggcgcag catcgctaga
    15661 gcgtacgacc gaagtcgtcc gggagattga aagaatcgcg ctcgatacgc cgggcatcgt
    15721 tcgcgttgcg gtgttcgcag gcttctcggg tgcgacccga acgcaggcgg gcaatgccgc
    15781 ggccttgttc ccggtgttcg aagagccgga cgtgcgcctg aagaaggggc tgtccgcggc
    15841 agtgatcaca ggcgagttgc gcaagcgcct cgccgcgctt cagggcgcgt tcatcatcgt
    15901 cattccgccg cccgcggtgc cgggcatcgg taccggcggc ggcttcacga tgcgcatcca
    15961 ggatcgccag ggtcgcggcc ctgaactcct ggcggcggcc accgacgaac tggtggcggc
    16021 ggcgcgcaag gcgcccggtc tcacctcggt gttttctccg ttcaccgcca atacgccgca
    16081 ggtgttcgtt gatatcgacc gcatcaaggc gcagaagctt ggcgtgccga tccagaatgt
    16141 gacggaaacg atccagacct atttcggctc ggcctacgtc aacgacttca acctgttcgg
    16201 ccgcacctat cacgtcacag cccaggcgga tttgccgttt cggaaggaaa ccaccgatct
    16261 cgcgcgcctg cgcacccgca atgccgccgg cgatatggtg ctgctcggca acgtggtcaa
    16321 tttccgcgat atatcgggac cggatcgcgt cgcgcgctac aacctgtatc cggcgtccga
    16381 actccagggc gatacgacgc cgggaacgag ttcggcgacc gccatcgaca ccatgaagcg
    16441 gctggcggcg gacaccttgc cgagtggctt cgcctatgag tggacggacc tgtcctatca
    16501 gcaggttacc gggggcaatt cgggtctcta tgtgtttccg atctgcgtgc tgttcgtgtt
    16561 cctggtgctg gccgcgcagt acggcagttg gagtttgccg ttcgccgtgc tgctgatcgt
    16621 gccgatgtgc ctgttggccg cgaccatcgg cgtgcggatc atgggacagg acgtcaatat
    16681 cctgacccag atcggttttg tggtcctcgt cggtcttgct gcgaagaacg cgattctgat
    16741 cgtggagttt gcccgcgaca tcgagcttga ggggcgcgag cgtctggcgg cggtgatcga
    16801 ggcctgccgg ttgcggctgc gtccgatatt gatgacatcg ttcgcgttca ttctcggtgt
    16861 gctgccgctg gtggtttcga ccggctcggg atcggaaatg cggcaggcgg tcggcgttgc
    16921 cgtgttcttc ggcatgctcg gcgtgacgct gttcggactg atcttcacgc cgatcttcta
    16981 cgtcatggtc cgcaacctgg ccgaccggcg gcacgacaag aaagccgttg caggcggata
    17041 gtgggctgcc gtcatcaatt ccacctcgcg cctccaagac tattggctta tttgaaatag
    17101 gtgggcagcc gcagggttat aaaataccat gttcggaata gtaacttgtt gccgggggag
    17161 acgaatatga agtcgggatt attggcggcc gttgcggcag gcgggctgtt actcgccggg
    17221 ccggcgtcgg cacagggcgt caagattggc atcctgaacg accaatcggg ggtctatgcg
    17281 gactatggcg gcaaatattc cgtcgaggct gccaaaatgg cggtcgagga gtttggcggc
    17341 agcgtgctcg gccaaaaaat agaagtcgtc tccgccgacc atcagaacaa agctgatctg
    17401 gccaccgcca tcgcgcggcg ctggtatgag gtcgaaggcg tcgacatgat caccgaactg
    17461 acgacgtcct cggtcgcgct cgccattcac gatctgtcga agcagatgaa gaagatcgac
    17521 atcgtggtgg gggccgcgac ctcgcgtctg accggcgacg cctgccagcc ttacggtttc
    17581 cattgggcct acgacaccca tgcgctcgcg gtcggcaccg gcggcgcgct ggtcgaagcg
    17641 ggcggcgata cctggttctt catgaccgcg gactacgcct tcggctatgc gctcgagaaa
    17701 gacaccggcg atctcgtcaa ggaaaagggc ggcaaggtgc tgggcgcggt ccggattccc
    17761 ttgaactcgt ccgacttctc gtcattcctg ctacaggccc agagctcgaa agccaaaatc
    17821 atcgggctcg ccaatgccgg tctcgacacc accaattcga tcaagcaggc ggccgaattc
    17881 ggcatcgtca agggcggcca aaaactcgcc ggactgttgc tgacgctggc cgaggtgcac
    17941 ggcctcggtc tcgaagccgc ccaaggcctg gtgctgaccg agggatacta ctgggatcgc
    18001 gacgacaaga gccgcgatct cgccaagcgc ttcttcgcgc gcaccggccg gatgccgaac
    18061 atgatccagg ccggcaccta ttcggcgacg ctgcagtact tgaaggccgt caaggcagcc
    18121 ggcaccaagg acaccgaggc ggtcgcccag aagctgaagg agcttccggt ggacgatctg
    18181 ttcgcgcaag gcggcaaggt gctggaaaat ggccgcatgg tgcacgacct ttatctgttc
    18241 gaggtcaaga agccgtcgga gtccaagaag ccctg
//
LOCUS       DATNOI010000585         3970 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_951504,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000585 DATNOI010000000
VERSION     DATNOI010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951504"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VN968_23655"
     CDS             complement(<1..288)
                     /locus_tag="VN968_23655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HXL32263.1"
                     /translation="MAVPPIRTEPRFSSTFDRYTISEIQRAAATGIYDIRGGGAKRKL
                     PHFDDLLFLGASISRYPLEGYREKCGTDVVLGSRFAKKPIHLRTPVTIAGMS"
     gene            complement(300..989)
                     /locus_tag="VN968_23660"
     CDS             complement(300..989)
                     /locus_tag="VN968_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein glxC"
                     /protein_id="HXL32264.1"
                     /translation="MPTHDLNVTAVRALNEALHRLSPDTNETFWQIENPQGKHAIGVG
                     IDAPVEVEINGHVGYYCGGMNKRATLRIRGNAGTGVAENMMSGLVHVMGDASQSAGAT
                     GCGGLLIIDGDAAARCGISMKGIDILVKGSIGAMSAFMAQAGCLVVLGDAGDALGDSI
                     YEATIFVRGEVKSLGTDCIEKPMTSDDRDRLADVLRRGGLNGEARVGDFRMYGSARRL
                     YHFHIDNIASY"
     gene            complement(989..1885)
                     /locus_tag="VN968_23665"
     CDS             complement(989..1885)
                     /locus_tag="VN968_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HXL32265.1"
                     /translation="MCGIVGLFLKNKNLEPALGDLLSRMLVTMSARGPDSAGVAIYGA
                     GDDDKVKLTLRVADATSVDDLRSELFAVVTSAFEISIRSSHIVLSVSRADAATVIHWL
                     KTHKSDLELVSVGETMEIYKEVGLPEHVVRVFGLAGMSGTHAIGHTRMATESAVTTNG
                     AHPFSTGEDQCLVHNGSLSNHNAVRRTLRREGMAFSTENDSEVAAGYLTWRMRQGASL
                     RDSLSSSLDDLDGFFTFVVGTRNGFAVLRDPIACKPAIMAESDDYVAFGSEYRALSVL
                     PNIEKARVFEPEPATVYAWEHR"
     gene            complement(2015..2671)
                     /locus_tag="VN968_23670"
     CDS             complement(2015..2671)
                     /locus_tag="VN968_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HXL32266.1"
                     /translation="MKRQSKPPFAEAGRDQADYRTGSNAPHEGVRSLERALGAKIRVI
                     RREKDLSVLDLSNASNISAGMLSKIENGQISPSLSTLQSIASALNVPLSLLFAASEER
                     RDCSFVRAKQGVVIERRGTKAGHVYQLLGHVIGGDVVVEPYLITLKEDAEAFTAFQHA
                     GMEFIYMLTGEVIYRHGDKTYRLRPGDSILFDSGALHGPETLLKRPMTYLSIIVYSRS
                     "
     gene            complement(2734..3324)
                     /locus_tag="VN968_23675"
     CDS             complement(2734..3324)
                     /locus_tag="VN968_23675"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HXL32267.1"
                     /translation="MRDLVHESAFAGLRAAGAGDGVVAIERLGLAIATVMQRRGQSRQ
                     LSAAVTANFAVDLPVGAEWTANNGVAWLGTGPGKWLAIGEARETEFVGKLKTKLGEFA
                     SVVEQSDGLGVLRLTGPALFRTLEKGVQVDLAPGAFPTGSVAVTSFAHIGVTLWKVDD
                     QPTVDVAVARSLCNSFLHWLEASAAPYGLTALGGQG"
     gene            complement(3317..>3970)
                     /locus_tag="VN968_23680"
     CDS             complement(3317..>3970)
                     /locus_tag="VN968_23680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HXL32268.1"
                     /translation="ADLSNEAFPYLACAELRLGQIPARLFRISFSGELAYEIAVPAGY
                     GGALAGRLADAGCEFGATPYGTEALSVMRIEKGHVAGNELNGQTAAGDLGLGRMMSTK
                     KDFVGRLMAARPALVDPQRPSLVGLKPVQPGDRLRAGAHFMARGVSPAPETDQGYMTS
                     TAFSPTLGHWIGLGLLARGAQRIGELVRAYDPVRSGDIEVEVVPAVFVDPEGVRVRA"
BASE COUNT          738 a         1278 c         1239 g          715 t
ORIGIN      
        1 actcatgccg gcgatcgtca ccggcgtcct gagatgaata ggcttcttgg cgaagcgcga
       61 ccccagcacg acgtcggtgc cgcacttctc gcggtagcct tcgagcggat agcgcgagat
      121 cgatgcgccg agaaacagca gatcgtcgaa atgcggaagc ttgcgcttgg cgccgccgcc
      181 gcggatatcg tagatgccgg tcgccgcggc gcgctggatt tccgaaatcg tgtagcggtc
      241 gaacgtcgac gaaaaccgcg gctcggttct gatgggaggc acggccatga attctccctt
      301 cagtaggatg cgatattgtc gatatggaaa tgatagaggc ggcgggccga tccatacatc
      361 cggaagtcgc cgaccctggc ctcgccattg agaccaccgc gacgcagcac gtcggccaga
      421 cgatcgcgat cgtcggatgt catcggcttt tcaatgcagt cggtgccgag gcttttgacc
      481 tcgccgcgca cgaagatcgt cgcttcatag atggagtcgc cgagcgcgtc gccggcatcg
      541 ccgagcacga ccaggcatcc ggcctgcgcc atgaaggcgc tcatggcgcc gatcgagcct
      601 ttcacgagaa tgtcgatgcc cttcatcgaa atgccgcagc gcgcggcggc gtcgccgtcg
      661 atgatcaaca ggccgccgca accggtggcg ccggccgact ggctggcatc gcccatgacg
      721 tgaaccagtc cggacatcat gttttcggca accccggtgc cggcattgcc gcgaatccgg
      781 agcgtggccc gcttgttcat gccgccgcaa taatagccga catggccgtt gatctcgacc
      841 tccaccgggg catcgatgcc gacgccgatc gcgtgcttgc cttgcgggtt ctcgatttgc
      901 cagaacgtct cgttggtgtc gggggagagc cgatgcagtg cctcgttgag cgcccgcacc
      961 gcggtgacgt tcagatcgtg cgtcggcatt accggtgttc ccacgcataa acggtcgcgg
     1021 gctcgggttc aaagacgcgg gccttttcga tatttggcag caccgacagc gcgcgatact
     1081 cggacccgaa cgcgacatag tcatcggatt ccgccatgat ggcgggcttg cacgcgatcg
     1141 gatcccgcaa gacggcaaag ccgttccggg tgccgaccac gaaggtaaaa aatccgtcga
     1201 ggtcatcgag gctggacgac agcgagtccc gcaggctcgc gccctggcgc atccgccagg
     1261 tcaggtaacc cgccgcgacc tcggaatcgt tctcggttga aaaggccata ccctcgcgcc
     1321 gcagcgtgcg gcggacggca ttgtggtttg acagggaccc gttgtggacc aggcactgat
     1381 cctcgccggt cgaaaacgga tgggcgccgt tggtggtgac ggccgactcg gtggccatgc
     1441 gggtgtggcc aatggcgtgg gtaccgctca tgccggcgag accgaatacc cggacgacat
     1501 gctcgggcag gccgacttcc ttgtagatct ccatggtctc gccgacgctg acgagttcca
     1561 gatcgctttt atgggttttc agccagtgga tcacggtcgc ggcgtcggcg cgcgaaaccg
     1621 acagcacgat gtgactggac ctgatgctga tctcgaacgc cgacgtcaca acagcgaaaa
     1681 gttcgcttct caggtcgtcc acggaggttg cgtcagccac gcgcagcgtc agcttgacct
     1741 tgtcgtcatc gcccgcgcca tagatcgcga cgcccgcgct gtcggggccg cgcgcggaca
     1801 tggtcaccag catgcgcgac agcaggtcgc cgagcgcggg ttcaaggttc ttgttcttca
     1861 gaaacagtcc tacaatgccg cacatgggcc tgatcctttg gtcggaccaa ctctagacgg
     1921 gccaaacagc gttcgtcaac caagaggaat gttattttcc tgataagaaa ataagcacat
     1981 ggtgcccttg tactggcaaa accgtcgggg gcctttagct tctcgagtaa acgatgatcg
     2041 acagataggt catcgggcgc ttgagcagcg tctcgggacc atgcaatgcg ccggaatcga
     2101 acaggatcga gtcgcccggg cgcagccgat aggtcttgtc gccgtggcgg tagatgacct
     2161 cgccggtcag catgtagatg aattccatgc cggcgtgctg aaatgcggtg aaggcctcgg
     2221 catcctcctt gagcgtgatc aaatagggtt cgaccaccac gtcgccgccg atcacgtgtc
     2281 caagcagttg atagacatgc ccggccttgg tgccgcgccg ttcgatcacg acaccctgct
     2341 tggcgcgcac gaaggagcag tcgcgccgct cttcggacgc cgcgaacaac aggctgagcg
     2401 gcacattcag ggcgctcgcg atcgattgca gcgtggaaag cgacggcgat atctggccgt
     2461 tctcgatctt cgacagcatg cccgcggata tgttcgatgc attggacaga tccagcaccg
     2521 acaggtcctt ctcgcgccgg atcacccgga tcttggcgcc gagtgcgcgc tccaggctcc
     2581 gtacgccctc atgcggcgcg tttgacccgg tccgataatc agcctggtcg cggccggctt
     2641 ccgcgaacgg tggcttggat tgccttttca tcgaagattc cccggtgaag cattttcctc
     2701 cctaacacat cccgcatgac tgccgacgat aattcagcct tgtccgccaa gcgccgtcag
     2761 gccgtacggc gcggccgacg cctccagcca gtgcaggaag ctgttgcaga ggcttctggc
     2821 caccgcgaca tcaaccgtcg gctgatcgtc caccttccac aaggtcacgc cgatatgcgc
     2881 aaagctggtg acggcgaccg acccggttgg aaacgcaccc ggcgccaggt ccacctggac
     2941 gcccttttcc agcgtcctga ataacgcggg accggtcagc cgcagcacac caaggccgtc
     3001 cgactgttcg accaccgagg caaattcccc aagcttcgtt ttgagtttgc ccacgaattc
     3061 cgtttcgcgt gcctcgccga tggcgagcca tttacccggt ccggttccga gccaggcaac
     3121 gccattgttc gccgtccatt ccgccccgac cggcaaatcg accgcaaaat tcgcggtcac
     3181 ggccgcggaa agctggcgcg attggcctcg gcgctgcatc acggtggcga tcgccaggcc
     3241 cagccgttcg atggcgacga cgccatcgcc cgcgccggct gcgcgcaggc cggcgaaggc
     3301 actttcatga accaggtcac gcacgaacac gaactccttc ggggtcgacg aataccgctg
     3361 gcacgacctc gacttcgatg tcgccggatc gcaccggatc gtaggcgcgg accagctcgc
     3421 cgatccgctg ggccccgcgc gcgagcaggc ccaatccgat ccagtggccg agcgtcgggc
     3481 tgaaggccgt cgaggtcatg tagccttggt cggtttcggg cgccggcgaa acgccgcgtg
     3541 ccatgaagtg ggcgccggcg cgaaggcggt cgcctggttg gaccggcttc agcccgacca
     3601 gactcggccg ctgcggatcg accagcgccg gccgcgccgc catcaggcgg ccgacaaaat
     3661 cctttttggt cgacatcatg cggccgaggc cgagatcgcc tgcggcggtt tggccgttga
     3721 gttcgttgcc ggcgacatgt cccttttcga tgcgcatcac cgacagcgcc tcggtgccgt
     3781 agggcgtagc accaaattcg cagccggcgt ccgccagcct gcccgcgagc gcgccgccat
     3841 atccggcagg cacggcaatc tcgtaggcca gctcgccaga aaacgagatg cggaacaggc
     3901 gcgccggaat ttgcccgagg cgcaactcgg cgcatgccag atagggaaac gcttcgttgg
     3961 aaagatcggc
//
LOCUS       DATNOI010000586         8315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2375706,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000586 DATNOI010000000
VERSION     DATNOI010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8315
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2375706"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VN968_23685"
     CDS             complement(<1..362)
                     /locus_tag="VN968_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HXL32269.1"
                     /translation="MSEESGASLINLQIMWHRLIAVVEEQAQVLLRTAFSPIVRECGD
                     LSAGVFDVKGRMLAQAVTGTPGHVNSMAQSVKHFIARFPILTMKQGDAYITNDPWMGT
                     GHLNDFVVTTPCFKDGRLV"
     gene            complement(365..2449)
                     /locus_tag="VN968_23690"
     CDS             complement(365..2449)
                     /locus_tag="VN968_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HXL32270.1"
                     /translation="MFQGAEVRLAVDIGGTFTDLVLDVGQDRRTRKVLTTPSRPEQAV
                     LDGMRLILTDARAHICDIDVFIHGTTLATNAIIERRGAKTALVATEGFRDVLDIGTES
                     RYDQYDLAIDKPKPLAPRSLRFTVPERIDAHGSVRLRLDETAVRALAPDLRAQNVESV
                     AFAFLHSYANPEHERRAAAVLKEELPAIWVTLSSAVCPEIREYERTSTAVANAYVQPL
                     IDGYLARMAEALQAEQFRGAIYLVTSGGGVTSIETARRFPVRLVESGPAGGAIFAAQI
                     AARLGETKVLSFDMGGTTAKICLIEQYQPETSRVFEVDRAARFLKGSGLPVRIPVIEM
                     VEIGAGGGSIARVDALKRVTVGPESASSEPGPACYGRGGQRPAVTDADVLLGKIDPDA
                     FAGGTIKLSPELSRRALLRAIGEPLGLTAETSAYAVHEVVCENMASAARVHAVERGAA
                     VGQHTLIAFGGAAPLHAARVAEKIGVSRVIVPSNAGVGSAVGFLAAPIAYELVRSRHV
                     RLDDFDAGAVSDLLHEMATEARALVEHGAGGAPVRERRAAFMRYVGQGHEITVELPNR
                     RLTEADLAGLRQSFEAHYAALFERPIPGAAIEVLSWSVLATTEPRNPPRVAEVTRKPA
                     GKAAGSRKFFDGRAGQVVEIPLYRRDQMAPGATIAGPAVIAEDGTSTFVSTSFNAHID
                     GAGSIVMERKAA"
     gene            complement(2592..3713)
                     /locus_tag="VN968_23695"
     CDS             complement(2592..3713)
                     /locus_tag="VN968_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXL32271.1"
                     /translation="MTSATDAPENPLPFGRSRSRRAGSRGAIGWLRANLFSSIPSTII
                     SLLLIFLLAKACVSLVQWGYWNAIWTVPGNQTSACRAIRGLGACWAVIPEKYRFILFG
                     TYPFDQQWRPALAILTFIALFFLSSRHSWWRRELVLVWVAALVVIGLLMWGGIFGLPY
                     VSQDRWGGLPVTLILATFGLAFGFPLGIVVALGRRSNLPAIRSLCVLYVEFIRGVPLI
                     SLLFMASVMFPLFMPDGVNIDKLLRAQIAFVLYAGAYLAEVMRGGLQAVPRGQHEAAD
                     ALGLSYWQKNGLIILPQAIRHVIPPLVNTFLAFFKDTSLVLIIGIFDLLTTAKTAIID
                     PVWQSFSIEVYLFVGSIYFAFCFAISRYSRRLEAQGKSA"
     gene            complement(3717..4904)
                     /locus_tag="VN968_23700"
     CDS             complement(3717..4904)
                     /locus_tag="VN968_23700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315607.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL32272.1"
                     /translation="MTTSTPAPARRKRLPISLSHERLAGLFWQIVVVGIAIAVVAWLW
                     SNAVHNLSARRISTGFAFLGREAGMPIADAWLSYSPQNTYLRAFIVGIVNMLRVAVIG
                     IVLATVLGTMIGIARLSSNWLLSRLAAVYVEVLRDLPLLLQLLFWYVLMQGLPAARAA
                     WMPIDGVYLSNRGLVLPSIPIGEGNLWVIGTVAVGLIALYALRRRLIARQLLDGQPRS
                     LWPYGLVLAIGLPALVSWGLGVSWTITKPELRDFNFVGGLTLTPEYFALVLALVTYTS
                     AFIAEIVRSGIQAVPRGQWDAAMALGLRRSFVLQHIIMPQALRVIIPPMTSQYLNLIK
                     NSSLAVAVGYQDIVSIANTTLNQTGQAIESIALIMLVFLTISLGISLFMNWYNARVAL
                     VER"
     gene            5003..5878
                     /locus_tag="VN968_23705"
     CDS             5003..5878
                     /locus_tag="VN968_23705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HXL32273.1"
                     /translation="MTQPQLKSSPLNELCSALPSLPMRLREVGQFVAANDYDATTRSM
                     RDLAAAAGADPASFTRLAKALGYSGWDGLRAALTEARRPAQSSPFSGRAKRRRQGPNS
                     DVSLVADKLEAEAAGLARISPGAVAAAAKALHAAQRIWIAGFRSCRGVAELLNYQLRL
                     FRPDTAQLVGGSGPEDLDLGAFRAGDAVVVIGFAPYSTASVLSARAAHRSGAGLIAIT
                     DTVIAPMAEGADHLLLFEAAASPGFFPSLTGAIAIAQSLAAVTFVLGGVGSKRRLEET
                     EARLAAMSQYVAEKG"
     gene            5884..7248
                     /locus_tag="VN968_23710"
     CDS             5884..7248
                     /locus_tag="VN968_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXL32274.1"
                     /translation="MGVSHSRVLHRSLRETPPKAVGGDGVWLIAEDGRRILDGSGGAA
                     VSCLGHQHPRVIEAISRQASKLAYAHTGFFSSEPAEALADLLVGDEPGGLAYAYLVSG
                     GSEAIEASIKLARQYFVESGQPQRQRFIARRQSYHGNTLGALSAGGNAWRRAPYAPLL
                     SPAFSHVTPAFAYHEKRDDESEADFVARLAAELEAEFQRLGPDTVAAFLAEPVVGATA
                     ACVPPPEGYFSAVRDICNRHGALLILDEVMCGMGRTGTLHAWEQEGIAPDIQAIAKGL
                     GGGYQPIGAMLASADIIDTIRRGSGAFQHGHTYLAHPLACAAALEVQRIIGEERLLER
                     VRDLGGHLERRLTERFGNHRHVGDIRGRGLFWAIELVADRASREPLDPALKLNQRIKA
                     AAFESGLVCYPAGGTVDGRSGDHVLLAPPYVATSDDIDMIVDRLGSAVDSALKTVGIG
                     GGQE"
     gene            7245..8258
                     /locus_tag="VN968_23715"
     CDS             7245..8258
                     /locus_tag="VN968_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL32275.1"
                     /translation="MKRIIIAAGLLTAWFSGATAGTLDTVKQRATLVCGVSAGFAGFS
                     TPDSQGNYKGLDVDYCRALAAGVLGDANKVRYVALAAQNRFTALQSGEIDVLYRNSTQ
                     TYLRGVTLGLRQGPVNFYDGQGFVVRRDSGVKDLKGLNGATVCVAQGTTHEVTLGDYG
                     RANGIEWKPLVFDRIDTMYQTFFGGRCDAMTQDASALAGAVATAAPNAADYLVLPLTI
                     SKEPLGPFTRNGDEVWTDIIAWLHYGLVEAEELGVTAANADEIAKSTSVPAIQRLLGS
                     SGELGSRLGLDNKWMVQAIKAGGNYAEIFERNVGQASPLKLNRGLNATWSKGGLMYAI
                     PFK"
BASE COUNT         1619 a         2695 c         2642 g         1359 t
ORIGIN      
        1 accagcctgc cgtccttgaa gcacggcgtg gtgacgacga aatcgttgag atggccggtg
       61 cccatccagg gatcgttggt gatgtaggcg tcgccttgct tcatcgtcag aatcgggaaa
      121 cgggcgatga aatgcttgac cgactgggcc atggaattga catgccccgg cgtgccggtc
      181 actgcctgcg ccagcatccg ccccttcacg tcgaacacgc cggccgagag gtcgccgcac
      241 tcgcgcacga tcggactgaa ggccgtgcgc agcagaacct gcgcctgttc ctcgaccacg
      301 gcgatcagcc ggtgccacat gatctgcaga ttgatcaggc tcgcgccact ttcctcgctc
      361 atggttacgc cgccttccgt tccatcacga tgctgccggc accgtcgata tgggcgttga
      421 agctcgtcga gacgaatgtc gaggttccat cttccgcgat cacggccggt cccgcgatgg
      481 tcgctccggg tgccatctgg tcgcggcggt acaggggaat ctcgacaacc tgccccgcgc
      541 gtccgtcgaa aaacttgcgg ctgcccgcag ctttcccggc cggcttgcgc gtcacctcgg
      601 cgacgcgcgg cggatttcgc ggctcggtgg tcgcgagcac cgaccagctc agcacctcga
      661 tcgccgcgcc tggaatcggc cgctcgaaca aggccgcata atgcgcctcg aacgattgac
      721 gcagacctgc gagatccgcc tcggtcaacc gccggttcgg cagttcgacc gtaatctcgt
      781 ggccttgtcc gacatagcgc atgaaggcgg cgcgccgttc gcgcaccggc gcgccgccag
      841 cgccgtgttc caccagggcg cgcgcctccg tcgccatctc atgcagcaga tcggacaccg
      901 ctccggcatc gaaatcatcg agcctgacat gacggctgcg caccagctca taggcgatcg
      961 gcgccgcaag aaatccgacc gccgagccga cgccggcgtt cgacggcacg atcacgcgcg
     1021 agacgccgat cttctcggca acgcgcgccg catgcagcgg cgcggcgccg ccgaacgcga
     1081 tcagggtgtg ctgcccgacc gcggcgccgc gctcgaccgc atgcacgcgg gctgcgcttg
     1141 ccatgttctc gcacacgact tcatgcacgg cgtaggccga cgtttccgcc gtcagtccca
     1201 gcggctcccc gatggcgcgc aacaaggccc gtcttgatag ctcgggactg agcttgatcg
     1261 ttcctccggc aaatgcgtcg ggatcgatct tgccgagcag gacgtccgca tcggtcacgg
     1321 cggggcgctg acctccgcgg ccgtagcagg cgggtccagg ctcggaagac gcgctctcgg
     1381 gcccgaccgt aacgcgcttc aacgcgtcga cccgcgcgat cgagccgccg ccggcgccga
     1441 tttcgaccat ctcgatgacg ggaatacgca ccggcaggcc cgaacctttc aggaagcgcg
     1501 cggcgcgatc gacctcgaac acgcgcgagg tctcgggctg atattgctcg atcaggcaga
     1561 tcttcgcggt ggtgcccccc atatcgaagg acaagacctt ggtttcgccg agacgagccg
     1621 caatctgcgc cgcgaaaatc gcgccgcccg ccgggcccga ctcgacaagg cgcaccggaa
     1681 agcgccgggc cgtctcgatc gacgtgacgc cgccacccga ggtcacaaga tagatggcgc
     1741 cgcggaactg ctcggcctgc agcgcctcgg ccatgcgcgc gagatagccg tcgatgagag
     1801 gctgcacata ggcgttggcg acggcggttg aagtccgctc atattcgcga atttcaggac
     1861 acaccgccga cgacagcgtc acccatatgg ccggcaattc ctccttcagg accgcggcgg
     1921 cgcgccgttc atgctcagga ttggcatagg aatggaggaa cgcgaaggcg acgctctcca
     1981 cgttctgcgc gcgcaggtcg ggcgcgagtg cgcgcacggc ggtttcatcg agccgcagcc
     2041 gcactgaacc atgcgcatcg atgcgctcgg gaacggtgaa gcgcagggag cgcggcgcca
     2101 gcggcttcgg cttgtcgatc gccaggtcgt attgatcgta gcggctctcg gtaccgatat
     2161 cgagcacatc gcggaaacct tccgtcgcga caagcgccgt cttcgcgcca cggcgttcta
     2221 tgatggcgtt ggtcgcgagc gtcgtgccat gaatgaagac gtcgatatcg cagatgtgcg
     2281 cgcgcgcgtc ggtcagaatg agacgcatcc catccagcac cgcttgctcg ggtcgcgacg
     2341 gcgtcgtcaa caccttgcgg gttctgcgat cctgacccac gtccagcacg agatcggtga
     2401 aagtgcctcc gatatcgacg gccagccgca cttcggctcc ttgaaacatt gccatatctc
     2461 cgtgccgcgg gctgaacgaa ctgagccaac agcgcagcaa tttccatgcc agtgattgcg
     2521 cgcggtgatg cgccgaacca ttttgctggg cacctatgca gcgccgcaac gagcgaaagc
     2581 caccctgccc gtcacgccga cttgccctgc gcttcgaggc gccggctgta gcgcgagatc
     2641 gcaaagcaga acgcgaaata aatcgatccg acgaacaggt agacctcgat gctgaacgac
     2701 tgccagacgg gatcgatgat ggcggtcttc gctgtcgtca gcaggtcgaa gatgccgatg
     2761 atcagaacca gactggtatc cttgaagaac gcgaggaagg tattcacgag tggcgggatc
     2821 acatgccgga tggcctgcgg caagatgatc agtccgtttt tctgccagta cgagaggccg
     2881 agcgcgtcgg ccgcctcgtg ctgtcctctg ggaacagcct gcaagccgcc gcgcatgact
     2941 tccgcaagat aggcgccagc gtaaagcacg aacgcgatct gggcccggag cagcttgtcg
     3001 atgttgacgc catcgggcat gaacagcgga aacatcacgc tcgccatgaa cagcaggctg
     3061 atcagcggaa caccacggat gaactcgaca tagagcacgc aaagtgaccg gatcgcgggc
     3121 agattggaac ggcggccgag cgcgacgacg atcccgagcg gaaagccgaa ggcgagcccg
     3181 aatgttgcga ggatcagcgt caccggtagc ccgccccagc gatcctggga aacataaggc
     3241 agcccgaaga tgccgcccca catcagcaag ccaatcacga ccagcgctgc gacccacacc
     3301 agcacgagct ccctgcgcca ccagctgtgc cggctcgaca ggaagaagag cgcaatgaac
     3361 gtgaggatcg caagagcggg ccgccattgc tgatcgaagg gataggtgcc gaacagaatg
     3421 aagcgatact tctcggggat aaccgcccag caggcgccaa gtccacggat cgcccgacaa
     3481 gcactggtct gattgccggg aaccgtccag atcgcgttcc agtaacccca ctgcacgagg
     3541 ctcacacagg ccttggcgag caggaagatc agcagcaggg agatgatggt cgagggaatc
     3601 gacgagaata gattggcgcg cagccatccg atcgcgccgc ggcttccggc cctgcgcgat
     3661 cgcgatcgac caaagggaag cggattttcc ggcgcatccg tcgctgaggt catcgatcag
     3721 cgctccacca gcgcgacgcg cgcattatac cagttcatga acaggcttat gccaaggctg
     3781 atggtcagga acaccagcat gatgagcgca atcgattcga tcgcctgtcc ggtctggttc
     3841 agcgtcgtat tcgcaatcga cacgatgtcc tgatagccga ccgcgacagc cagcgatgaa
     3901 ttcttgatca aattcagata ttggctggtc atcggcggaa tgatgacgcg aagcgcctgc
     3961 ggcatgatga tgtgctgaag aacgaagctc cggcgcagac cgagcgccat ggcggcatcc
     4021 cattgccccc tgggaacggc ctgtatgccg ctgcgcacga tctcggcgat gaaggccgag
     4081 gtataggtga cgagcgccaa cacgagcgcg aagtactccg gcgtcaaggt caatccgccg
     4141 acaaaattga agtcccgcag ctccggcttg gtgatggtcc aggatacgcc caggccccac
     4201 gacaccagcg ccggcagccc gatcgccaga acaagcccgt aaggccagag ggaacgcggc
     4261 tggccatcca acagctgtcg cgcgatcaat cgccggcgca gcgcatacaa cgcaatcagc
     4321 ccgaccgcga ccgtaccgat cacccaaagg tttccttccc cgatcggaat cgacggcagc
     4381 accagacctc gatttgaaag atatacgccg tctatcggca tccatgcggc gcgggccgct
     4441 ggaagtccct gcatcagcac gtaccagaac aagagctgca gcagcagcgg aagatcacgg
     4501 agcacttcga catagaccgc cgccagccgg gacagcagcc aattggacga cagccgcgcg
     4561 atgccgatca tcgtcccgag cacggtcgca agcacgatgc cgatgacggc gacacggagc
     4621 atgttgacga tgccaacgat gaaggcgcgc agataggtgt tctgggggct gtaggaaagc
     4681 caggcgtcgg caatcggcat cccggcctcg cgaccgagaa aggcaaagcc ggtcgagatg
     4741 cggcgcgccg acagattgtg aacagcattc gaccagagcc aggcgacgac agcgatggcg
     4801 atccccacca ccacgatctg ccagaaaaga ccggcgaggc gctcatggct cagtgaaatg
     4861 ggcaaccgct tgcggcgagc gggtgcgggc gtggatgtcg tcacagcaca ggatccttcc
     4921 ggcagcagcg atcttcggcc atgcgcaagc ggggcgcgct tcatatgttg cacagaaagg
     4981 ctttttgtgc aacatacgtt tcatgaccca gccgcagttg aagtcctcgc ccctcaacga
     5041 actctgcagc gcgttgccgt cgctgccgat gcggttgcgg gaagttgggc agtttgtcgc
     5101 cgccaacgac tatgacgcca cgacgcgttc gatgcgcgac ctggccgccg cggcgggagc
     5161 cgatcccgca tccttcaccc ggctggcgaa ggcgctgggt tattcgggat gggacggact
     5221 ccgcgccgcc ttgaccgagg cgcgccggcc cgcgcaatcg tctccgttct caggccgagc
     5281 gaagcggcgg cgccagggtc ccaattccga cgtctcgctg gtcgccgaca agctcgaagc
     5341 cgaggcggcc ggccttgcgc ggatctcgcc tggcgcggtg gccgcggccg ccaaggccct
     5401 gcatgccgca caacgtatct ggattgccgg ctttcgaagc tgccgcggcg tcgcggaact
     5461 gctcaattac cagcttcggc tgttcaggcc cgacaccgcg cagctggttg gcggctcggg
     5521 tccggaagat ctcgacctcg gcgccttccg tgccggcgac gccgttgtcg tcatcggctt
     5581 tgcgccctac tcgacggcaa gcgtattatc ggcccgcgcg gcgcatcgct ccggcgccgg
     5641 gctgatcgcg atcaccgaca cggtcatcgc accgatggcg gaaggcgccg atcacctgtt
     5701 gctgtttgaa gccgccgcct cgcctggatt tttcccgagc ctgaccggtg cgattgcgat
     5761 cgcgcagtcg ctggccgcgg tcaccttcgt actgggtggc gtgggctcga agcggcgcct
     5821 cgaagagacc gaagcccgcc tcgcggcaat gtcgcaatat gttgccgaga aaggttgagc
     5881 gtcatgggcg tcagccacag ccgcgtgctg caccggagcc tgcgcgagac gccgccaaaa
     5941 gccgtcggcg gcgacggcgt gtggctcatt gccgaggatg gccgccggat tctcgatggc
     6001 tccggcggcg ctgccgtttc ctgtctcgga catcaacatc cgcgcgtgat cgaagccatc
     6061 tcaaggcagg cgtcgaagct cgcttatgcc catacaggct tcttctcgtc tgagccggcg
     6121 gaggcgctgg ccgacctgct cgtcggcgac gaacctggcg gcctcgccta cgcctacttg
     6181 gtcagcggcg ggtcggaagc gatcgaagcc agcatcaaac tggcgcggca atattttgtc
     6241 gaaagcggtc agccacaacg ccagcgcttc atcgcccgtc gccagagcta ccatggcaat
     6301 acgctcggcg cgctgtcggc gggcggaaac gcctggcgcc gcgcgcctta tgcgccattg
     6361 ctgtcgccgg cgttcagcca tgtgacgcct gccttcgcct atcacgagaa acgcgacgac
     6421 gagtcggaag cggatttcgt ggcgcggctg gcggccgaac tggaggctga attccagcgc
     6481 ctcggcccgg acacggtggc cgcattcttg gcagagccgg tggttggagc caccgccgca
     6541 tgtgtgccac cgcccgaagg ttacttcagc gccgtgcgcg acatctgcaa ccggcacggc
     6601 gccctgctca tcctcgacga agtgatgtgc ggcatgggcc gcacgggcac gcttcacgcg
     6661 tgggaacagg agggcattgc gcccgacatc caggccatcg ccaagggatt aggcggcggc
     6721 taccagccga ttggcgcgat gctcgcaagc gctgacatca tcgacacgat caggcggggc
     6781 tcgggagctt tccagcacgg acacacctat ctcgcgcacc ctctcgcctg cgccgcggcg
     6841 cttgaggtgc agagaattat tggtgaagag agactgctcg aacgggtcag ggatctcggc
     6901 ggtcatctcg agcgacggtt gaccgagcgc ttcggcaatc accggcacgt cggcgatatc
     6961 cgcgggcgcg gcctattctg ggccatcgaa ctggtcgccg atcgcgccag ccgggagccg
     7021 ctcgatcctg ccctcaagct caaccagcgg atcaaggccg ccgcgttcga aagcggcctc
     7081 gtctgttatc cggcaggcgg gacggtggac ggccgcagcg gcgaccacgt cctgctcgca
     7141 ccgccttacg tcgcgacatc agacgacatc gatatgatcg tcgacaggct cggttctgcg
     7201 gtagacagcg cattaaaaac tgttggcata ggaggaggac aagaatgaag cgaatcatca
     7261 ttgctgcagg attgctcacc gcatggtttt cgggagcaac cgccggaacg cttgacacgg
     7321 tcaagcagcg cgccacgctg gtgtgcggcg tcagcgcggg ctttgccgga ttctccactc
     7381 cagactccca gggcaattac aagggactcg acgtcgacta ctgccgtgcg ttggcagcgg
     7441 gggttctcgg tgacgccaac aaggtacgtt atgtcgcgct cgcggcgcaa aaccggttca
     7501 ccgcgctgca atcgggcgag atcgacgtgc tctatcgcaa ttcgacgcag acctatctgc
     7561 gcggcgtaac cctcggcctg cggcagggac cggtcaattt ctacgacggc cagggttttg
     7621 tcgttcggcg cgacagcggc gtgaaggatc tgaaggggct gaacggcgcc accgtttgcg
     7681 tcgcgcaggg aacgacccat gaggtgacgc tcggcgatta cggtcgcgcc aatgggatcg
     7741 aatggaagcc gctggtgttc gatcgcattg acaccatgta tcagacgttt ttcggcgggc
     7801 gttgtgacgc catgacccag gacgcctccg cgctcgccgg cgcggttgca acggcggcgc
     7861 cgaacgctgc cgactacctc gtcctgccgc tgacgatcag caaggaaccg ctcgggccat
     7921 tcacgcgcaa tggcgacgag gtgtggaccg acatcatcgc ctggcttcac tacggcctcg
     7981 tcgaggcaga ggaactcggc gtgaccgcag ccaatgcgga cgaaattgcc aaatccacca
     8041 gcgtaccggc gatccagcga ctgctcggca gctccggaga actcggatcg cgccttggcc
     8101 tcgacaacaa gtggatggtg caggcaatca aggcgggcgg caactacgcc gagatcttcg
     8161 agcgcaacgt cgggcaggcg agcccgctga agctcaatcg cggcctgaac gcgacctgga
     8221 gcaagggcgg cttgatgtac gccatcccct tcaaataagg gccgcggctc ggcgggtaat
     8281 attgacataa gctcggcgcg gccaggataa acaag
//
LOCUS       DATNOI010000587         9844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1663999,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000587 DATNOI010000000
VERSION     DATNOI010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9844
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663999"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1069
                     /locus_tag="VN968_23720"
     CDS             <1..1069
                     /locus_tag="VN968_23720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32276.1"
                     /translation="INSKVAKANATAEDPWAIAWLKEHTAGSGAYMIESFKPGEQVIM
                     KRNEAWNRGSPEKSAFFKRVIVQSVPEPATRANLVERGDADLVIDLQASDVQSLEAKG
                     TLKVISTPQYNAVTFISMNNQIPPFDNINVRRAIAYALPYDDMFKAALFGRGTPLFGA
                     MWADGKPTTSTYPFQQPVKLDLDKAKEYLKAAGMPDGFSTTFSFNVGQASTAEPMSAL
                     VKESLAKIGVKVDIQKLPDAQMSTQINEKKLPFFTEGIVAWLPSTDYFYRNFYTGEQR
                     WNYSSIKNTELTAIAQQARFEPDKAKYDEYGKKLNAIHFAEMPQIPLWQPNQDAVMAT
                     SIDGYTYQFHRMVDYRDLHRK"
     gene            1082..2113
                     /locus_tag="VN968_23725"
     CDS             1082..2113
                     /locus_tag="VN968_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL32277.1"
                     /translation="MKSFGATMMWAGRRFLSSLPALFGVLVFTFLLMRVLPGDPAVFF
                     ASGPNAGKEEIAVIRKQMGLDKPVPNQLLLYLYDVGRGNLGRSIMTGQPVTTDLRNRL
                     PASLELTLSALLIALVSAIPLGVLAALKPGSVIDHGVRLFCALGVCVPTFVSGLLLIY
                     VFYYLLGIAPDPTGRLDVFTSLPPSRTGFLMIDFALAGDIDGWWAAFRQLILPAVTMA
                     LFVIAPLARITRASMLVSLGSDFVRTARSAGLSWWRIVVTYALRNAILPVITIAGIVF
                     STMLGANVLVEKVYSWPGVASYALDALLSSDYAPVQGFVLLMASVFVVVNLLVDILYG
                     IADPRVSIG"
     gene            2110..2958
                     /locus_tag="VN968_23730"
     CDS             2110..2958
                     /locus_tag="VN968_23730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL32278.1"
                     /translation="MTGTTLRHAAWILRGNPLTAIAAAGVVLLVVIAVFGPQIVPYDP
                     IASNVSQALTPPSAAHLAGTDQLGRDTFSRLIVATRLDLVIAVSAVGISFVLGAVIGA
                     FCGYSGGGLDRTVGRFVDVMMAFPLFVLAMAMVAALGNRVENIVIATAVINLPFYIRF
                     ARAEVNIRRNVGWVEAARASGESHISVVLRFLLPNVLPAMAVQMSLNLGWAILNAAGL
                     SFIGLGVKPPTPEWGIMVAEGARFISTGRWWLVAFPGLALMLAVLCFNLLGDGMRDIL
                     DPRMRT"
     gene            2955..4628
                     /locus_tag="VN968_23735"
     CDS             2955..4628
                     /locus_tag="VN968_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32279.1"
                     /translation="MTEPLLRIEDLHVTFSTRRGLVEAVRGVTLSLDAGEMLGLVGES
                     GSGKSVTGFATTRLLDAAGRVTAGRILFRGQDITKIPDGDFRMLHGAAISMIFQNPRA
                     ALNPIRAIGQQIADAILSHKRMSREQAAGEALELLRAVQIRDPEKRMAAYPHELSGGM
                     CQRVMIAIAISCAPMLLIADEPTTGLDVTTQKVVMDLLAGIAAQRGMATILITHDLGL
                     AVRYCRRVVVMEQGRLVEEAPPQALFRNPRHVYTKRLVAASPTATSRIEDLVTDEEKS
                     RYLAKLAACKPRPAPPPGTPLLLDVQSLAKRYDQGSPAVTDFSMTMAAGESVGLVGES
                     GSGKTTTSRMICRLIDPSEGVILFDGQSIGDLAARDFHCSPVRKEIQLVFQDPNDSLN
                     PRFTAFDCIAHPLLRLAGMRPGDALRQRVEECAQRVGLPLGLLPRFPHQLSGGQKARI
                     GIARAIACRPRLLVLDEPTAALDVSVQAVVLQLLYRLRREDNLAFLFVSHDLNVVRMM
                     CDHTIVLRNGCIVEQGESRAVFGNPQTAYTRELVEAVPHIQKELAAAGT"
     gene            complement(4722..5693)
                     /locus_tag="VN968_23740"
     CDS             complement(4722..5693)
                     /locus_tag="VN968_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL32280.1"
                     /translation="MPGRFLRELTIRQLRALAAVQKHRSVTAAAKQLHLTQPAVTLQL
                     RNLQALAGLPLIQRTSDGMLLTDAGREVLALSERIEAAIASCETSLDMIAGKTAGRIS
                     IGAVSTAKYFVPFAISGFSQAYPNVDVALSIGNRQEIGTALRGYDLDIAIMGRPPVDI
                     PMNVHLIGDHPHVIIAPTAHRLARKARLSLSALSDETFLTREPGSGTRGLMEQLFEQA
                     GVRPKIGMAMSSNETIKQAVIAGLGIAFISAHTVATELDERRLVTLDVVGLPVVRQWF
                     VLSRKDKVLLPPAQAMLDFLSDKGAQFLPRTHGRLRLTRPSAPRKLA"
     gene            5839..6876
                     /locus_tag="VN968_23745"
     CDS             5839..6876
                     /locus_tag="VN968_23745"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HXL32281.1"
                     /translation="MHERATLRSHLDRSTSQTPHGVAVGEAIAAIAAAAVDLAALIAD
                     GPLAGITGENGGTNADGDQQKDIDVAADEMMRRALRGAPVAAILSEEAALPEIINSAA
                     PLCVAIDPLDGSANLENNISVGTIFSIRPRGNDILSTFFEPGTAQCAAGFVVYGPQTT
                     LVLALNARVDIFILDRRTREFRLITQGVRVAPDTPEFAINASNRRHWHGPVRAYIDEC
                     IAGTNGGGEADFNMRWIGSLVAESLRILVRGGVFLYPADARPGYREGRLRLLYEAHPM
                     ALVMEWAGGAATTGRRRILELGAKTPHQRVPLIMGSLRGVRDVTTIHEGVEPMFDNSD
                     APLFARRGLFL"
     gene            6891..7766
                     /locus_tag="VN968_23750"
     CDS             6891..7766
                     /locus_tag="VN968_23750"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HXL32282.1"
                     /translation="MSRSHPIISITGSSGAGTTSVKKTFENIFRREKVSAAYIEGDAF
                     HRYNRAEMRTKMAEEGERGNKHFSHFSPETNLFEELERLFRDYGESGTGTTRHYVHDD
                     DEAKLYGAAPGTFTEWAPLPEKSDLLFYEGLHGAVVTDKVNIAQHADLKIGVVPVINL
                     EWIQKLHRDRSDRGYTQEAVTDTILRRMPDYVNYICPQFAETDINFQRVPTVDTSNPF
                     IARWIPTPDESMVVIRLRNPRGIDFPYLLSMIPNSFMSRANSIVIHGSKLDLAMQLIL
                     TPLILQLMERKRRTV"
     gene            7763..9802
                     /gene="tkt"
                     /locus_tag="VN968_23755"
     CDS             7763..9802
                     /gene="tkt"
                     /locus_tag="VN968_23755"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXL32283.1"
                     /translation="MSIHALSSFAGCSDGSHQELANAIRFLAIDAVEKAKSGHPGMPM
                     GMADVATVLFSRFLKFDPADPEWPDRDRFVLSAGHGSMLLYALLHLTGYEGVTSDELK
                     AFRQWGSKTPGHPEYGHTPGVETTTGPLGQGIATAVGMALAERLLNARFGDDLVDHYT
                     YVIAGDGCLMEGLSHEAISLAGHLGLGKLIVLFDDNQISIDGSTSLSCSDDQLARFKA
                     SGWSADRIDGHDPQAIATAIERARSNDLPSLIACRTVIGFGAPNRQGTEKAHGAPFGA
                     EEVAKTRVALDWPYEPFQLPEHIVAQWHKIGARGQAARLSWMGRTRRLNSVPRSPLHD
                     ALNRKLPCEYADAIDQICTRFGAERPTIATRQASQLVIDAIAEALPNLLGGSADLTHS
                     NLTRAKTQRPVRADSFEGNYIHYGVREHAMAAAMNGIALHGGFIPYGGTFLTFADYSR
                     PAIRLAALMGIRVVHVMTHDSIGLGEDGPTHQPVEHLASLRAIPNLLVFRPGDAVETA
                     EAWDCALRSEASPSVLCLSRQALPAFREVNDETNLVAFGAYVVVEPEDGRDVTLIATG
                     SEVSIAIEAAKLLAKDSVRAAVVSAPCFDLFRQQSREYRTAVLGRVPRIGVEAAIEGD
                     WARWLGDGGEFVGMTGFGASAPAEVLYREFGITAQAVATAALHCLVRSKMPATHP"
BASE COUNT         1809 a         3034 c         3056 g         1945 t
ORIGIN      
        1 catcaactcc aaggttgcga aggcgaatgc gacggccgaa gatccctggg cgatcgcctg
       61 gctcaaggag cacacggccg gcagcggcgc ctacatgatc gagagcttca agcctggcga
      121 gcaggtgatc atgaagcgca acgaggcctg gaatcgcggc tcgccggaaa aatccgcttt
      181 cttcaagcgg gtcatcgtgc aatcggtacc tgagccagcg acccgcgcca acctggtcga
      241 gcgcggcgat gccgatctgg tgatcgatct gcaggccagc gacgtccagt cactggaagc
      301 caaggggact ctcaaggtga tctcgacgcc gcaatacaat gccgtgacct tcatttcgat
      361 gaacaaccag attccaccct tcgacaacat caacgtacgc cgcgccatcg cctatgcgct
      421 gccttacgat gacatgttca aggcggctct gttcggccgc ggcacgccgt tgttcggtgc
      481 gatgtgggca gatggcaaac cgacaaccag cacttatccc ttccagcagc cggtgaagct
      541 cgacctcgac aaggcgaagg aatatctcaa ggcggcaggc atgcccgacg gtttctcgac
      601 cacgttcagt ttcaacgtcg gccaggcctc gacagccgaa ccgatgtcgg cgcttgtcaa
      661 ggaatccctc gccaagatcg gcgtcaaggt cgatattcag aaattacccg atgcgcagat
      721 gtcgacgcag atcaacgaga agaagctgcc tttcttcacc gaaggcattg tcgcctggct
      781 gccctcgacc gactatttct accgcaattt ctataccggc gagcagcgct ggaattacag
      841 cagcatcaaa aatacagagc tgacggccat cgcgcagcaa gctcgcttcg aacccgacaa
      901 ggccaagtac gacgaatacg gcaagaagct caacgcgatc catttcgccg agatgccgca
      961 gattccgctt tggcagccca atcaggacgc ggtgatggcg acgtcgatcg acggctacac
     1021 ctatcagttt caccgcatgg tcgattatcg cgacctccac cgcaagtaaa cggccggctt
     1081 catgaagagc ttcggcgcaa cgatgatgtg ggcagggagg cggttcctgt cctcgctgcc
     1141 tgcgctgttc ggcgtgctgg tctttacctt cctgctgatg cgggtgctgc caggtgatcc
     1201 cgcggtgttc tttgcctccg gtccaaatgc cggcaaggag gaaatcgcgg tcatccgcaa
     1261 gcagatggga ttggacaagc cggtaccaaa ccaattgctg ctttatcttt atgatgtcgg
     1321 tcgcggtaat ctcggccggt cgatcatgac cggacagccg gtcacgactg atctccgcaa
     1381 tcggttaccg gcctcgctgg agcttaccct ctccgcactg ctgattgcgc ttgtatcggc
     1441 gatccctctc ggcgtgctgg cggcgttgaa gcctggctcc gtcatagacc atggcgtgcg
     1501 attgttttgt gcgctgggcg tctgcgtccc aacctttgtt tcgggtctct tgctgatcta
     1561 cgtcttctac tacctgctcg gtatcgcacc cgatccgacc ggacgtctcg acgtctttac
     1621 ctcgttgccg ccatcgcgaa ccggtttcct gatgatcgac ttcgcgttgg cgggggacat
     1681 cgatggctgg tgggcggctt tccgacagct gatcctgccg gcagtgacca tggcgttgtt
     1741 cgtgatcgcg ccgctggcgc gaatcacgcg cgcgtcgatg ctggtctcgc tcggcagcga
     1801 tttcgtgcgc accgcgcgct cggccgggct ttcctggtgg cgcattgtcg tgacctatgc
     1861 gttgcgcaac gctatcctgc cggtcatcac catcgcaggc atcgtattct cgaccatgct
     1921 cggagccaat gtgctggtgg agaaggtcta ttcctggcca ggcgtcgcct cctatgcgct
     1981 ggatgcgctg ctgtcgtccg actatgcgcc ggtccagggc tttgtgttgc tgatggccag
     2041 tgtatttgtg gtggtcaatc tgctcgtgga tattctctac gggattgccg atccgcgggt
     2101 gtcgatcgga tgaccggcac gacgcttcgt cacgcggcct ggatcctgcg cggcaaccct
     2161 ttgaccgcaa tcgccgcggc cggtgtcgtc cttctggtcg ttatcgccgt gtttgggccg
     2221 cagatcgtac cctacgatcc gatcgcctcg aatgtgtcgc aggcgctgac gccgccaagc
     2281 gcggcgcatc ttgccggcac cgaccagctc ggccgcgata cgttcagtcg gctgatcgtt
     2341 gcgacacgat tggatctcgt catcgccgtt tcggccgtgg gcatctcctt cgtgcttggg
     2401 gccgtcattg gcgcgttctg cggctattcc ggcggtgggc tggatcgcac cgtcggccgt
     2461 ttcgtcgacg tgatgatggc cttcccgcta ttcgtgctgg ccatggcgat ggtcgcggcg
     2521 ctcggcaatc gggtggagaa catcgtcatc gcgactgccg tgatcaatct gccgttctac
     2581 atccgctttg cgcgcgcgga ggtgaacatc cgccggaatg tcggctgggt agaggcggcg
     2641 cgcgccagcg gcgaaagcca tatctcggtg gtgctgcgtt tcctgctgcc gaatgtgctt
     2701 ccggccatgg ccgtacagat gtcgctcaac cttggttggg ccattctcaa tgccgccggg
     2761 ctctccttta tcggcctcgg cgtcaagcct ccgaccccgg agtggggcat catggtcgcg
     2821 gagggcgcac gcttcatttc gaccggcaga tggtggctgg tcgcatttcc gggactggcg
     2881 ctgatgctgg ccgtgctgtg cttcaatttg ctcggcgatg ggatgcggga cattctcgat
     2941 ccccgcatgc ggacatgact gagccgctgc tgcgaataga ggacctgcac gtcacgttct
     3001 cgacacgccg cggccttgtc gaagcggtgc gcggagtgac gctttcgctc gatgcgggtg
     3061 aaatgctcgg gctcgtcggc gagagcggtt ccgggaaatc cgtcaccgga tttgcgacca
     3121 cgcggctgct tgacgcggcc gggcgggtga cggctggcag gattctattt cgcgggcagg
     3181 acatcactaa aataccggat ggcgacttcc gcatgctgca tggcgccgcc atctcgatga
     3241 tctttcagaa tccgcgcgcc gcgctcaacc cgatccgcgc catcggccag cagatcgccg
     3301 atgcgatcct gagccacaag cggatgtcca gggaacaagc ggccggcgaa gcgctggagc
     3361 tcttgcgcgc ggttcaaatc cgcgatcccg aaaagcgaat ggcagcctac ccgcacgagc
     3421 tctccggcgg aatgtgtcag cgggtgatga tcgcaatcgc gatttcctgc gccccgatgc
     3481 tgctgatcgc ggacgagccg acgaccggcc tcgacgtcac cacgcagaag gtggtgatgg
     3541 acctgctggc cgggattgcg gcacaacgcg ggatggcgac gatcctgatc acgcatgatc
     3601 tcggcctcgc ggttcgctat tgtcgccgcg tcgtggtgat ggaacagggc cgcctcgtcg
     3661 aggaagcgcc gccgcaggcc cttttccgca acccgcggca tgtctacacc aagcgcctgg
     3721 tggccgcctc gccgacggca acctcgcgta tcgaggacct ggtgacggac gaagagaaaa
     3781 gtcggtacct cgccaagctc gccgcgtgca aaccccggcc ggcgccgccg ccgggcacgc
     3841 cgcttctgct ggatgtgcag agccttgcca agcgctatga tcaaggctcg ccggcggtga
     3901 ccgacttttc catgaccatg gccgccggcg agagcgtcgg gctggtcggc gaatccggtt
     3961 ccgggaagac cacgacgtcg cgcatgattt gccggctgat cgaccccagc gaaggcgtca
     4021 ttctgttcga cgggcaatcg atcggcgatc tggccgcgcg ggatttccac tgctctccgg
     4081 tacgaaagga aatccagctc gtctttcagg atcctaacga cagcctgaac ccgcgtttca
     4141 ccgcctttga ttgcatcgcc catccgctac tgcgacttgc gggcatgcgc ccgggcgacg
     4201 cgttgcgaca gcgggttgag gaatgcgcgc aacgggtggg attgccattg gggcttctgc
     4261 cgcgcttccc gcatcaactc tcgggcggcc agaaggcccg catcggcatc gcccgcgcca
     4321 tcgcctgccg gccgcgcctg ttggtgctgg atgagccgac ggccgcgctc gacgtctcgg
     4381 tgcaggcggt ggtgctgcaa ctgctgtaca ggttgaggcg cgaggataac cttgccttcc
     4441 tctttgtcag tcacgacctc aacgtggttc gcatgatgtg cgatcacacc atcgttctgc
     4501 gcaacggctg catcgtcgag cagggcgaga gccgggcggt gttcggcaac ccacagaccg
     4561 cttacacacg cgaactggtc gaagcggtgc ctcatatcca gaaggagctg gctgcagccg
     4621 ggacttgata ttttacgtct acgagcttgg gtgaccgtgc cgtcgttgaa tgtcgcgatc
     4681 cgcggcgcgg gcgcaggtga gcagcgtctc cgcggcgggc gtcatgcaag cttgcggggt
     4741 gctgatggcc gggtgaggcg aagcctgccg tgggtgcgcg gcagaaattg agctcccttg
     4801 tcgctgagaa agtcgagcat ggcctgcgcg gggggcagca gcaccttgtc cttgcgggaa
     4861 agcacaaacc attgccggac caccgggaga ccaacgacgt ccagcgtcac cagccgtcgc
     4921 tcgtcgagtt cggttgcgac cgtatgcgct gaaataaagg caatgcccaa accggcaatc
     4981 accgcctgtt tgatggtctc gttgctgctc atcgccatgc cgattttcgg gcgaacaccg
     5041 gcctgttcga acagttgctc catcaagccg cgtgttccgg atccgggctc gcgtgtcagg
     5101 aatgtttcat cggaaagggc actcagcgac agacgcgctt tccgcgccag gcgatgcgcg
     5161 gtcggcgcga tgatgacatg tgggtgatcg ccgatcagat gaacgttcat ggggatatcc
     5221 accggtgggc ggcccatgat ggcgatatcc agatcgtagc ctcgcaacgc tgtgccgatt
     5281 tcctgccggt tgccgatcga gagcgcgaca tcgacgttcg gatacgcctg cgaaaatccg
     5341 gagatcgcga acggcacgaa atatttcgca gtactgacgg cgccgatgga aatacgcccg
     5401 gcggtctttc ccgcgatcat gtcgagcgag gtttcgcagg acgcaattgc cgcttcgatc
     5461 cgctcggaaa gcgccagcac ttcacggccg gcatccgtca gcagcatgcc atcactggta
     5521 cgctggatca gcgggagtcc cgccaacgcc tgcaagttcc gaagctgaag ggtgaccgcg
     5581 ggctgggtga gatgaagttg cttggctgcc gcggtcaccg accgatgttt ttgaacggcc
     5641 gccaatgccc gcaactggcg gatggtcaat tcccgcaaaa accggcccgg catcgcaacc
     5701 accttataag aaaaactttg ctctctccaa agataacaaa atttcactaa tctggcaaat
     5761 tgcgcttctc tgaataggcg gaccgccgaa cggcgaaacg cagtgcgagc accccgaaga
     5821 gggcgcaggg agacggccat gcacgagcgc gcgaccttgc gttcgcatct cgatcggtcc
     5881 acctcgcaaa cgccgcatgg cgtggcggtg ggcgaggcga tcgcggccat tgctgccgcc
     5941 gcggtcgacc ttgccgccct catcgcggac gggccgctgg cgggcatcac cggcgagaac
     6001 ggcggcacca atgccgatgg cgatcagcaa aaggatatcg atgtcgcagc cgacgaaatg
     6061 atgcgtcgcg cgctgcgggg ggccccggtc gctgcgattc tttccgaaga ggcggcgcta
     6121 ccggaaatca tcaattccgc ggcgccgctc tgcgtcgcca tcgatccgct cgacggatcg
     6181 gccaaccttg aaaacaacat ctcggtcggc accattttct cgatccgccc caggggcaac
     6241 gacatcctct cgaccttctt tgaaccgggc acggcgcagt gcgcggcagg tttcgtcgtc
     6301 tacggtccgc aaaccacgct cgtgctcgcg ctcaacgcgc gcgtcgatat cttcattctc
     6361 gacaggcgta cgcgggaatt ccggttgatc acccagggcg tccgcgtcgc accggatacg
     6421 ccggaattcg ccatcaacgc ctccaaccga aggcactggc acgggccggt tcgggcctac
     6481 atcgatgaat gcattgccgg taccaacggc ggcggcgagg cggatttcaa tatgcggtgg
     6541 atcgggtcgc tggtcgcaga atcgctgcgc atcctggtgc gcggcggcgt gtttctttat
     6601 ccggccgatg cccgccccgg atatcgcgaa ggcaggcttc ggcttctgta tgaagcgcat
     6661 cccatggcgc tggtgatgga gtgggcggga ggcgctgcga cgacggggcg ccggcgaatt
     6721 ctggaactcg gagcgaaaac gccgcatcag cgggtgccgc tgattatggg atcactgcgt
     6781 ggcgttcgcg acgtcaccac cattcacgaa ggtgtcgaac cgatgttcga caatagcgac
     6841 gcaccgctgt ttgcgcggcg cggtctgttt ctctgagctg gacaggtccg atgtcgcgca
     6901 gccatcccat catatcgatc acgggttcat cgggcgccgg cacgacgtcg gtcaaaaaaa
     6961 cgttcgaaaa tatcttccga cgcgaaaagg tatccgcggc ctatatcgaa ggcgacgctt
     7021 tccatcgcta caatcgcgcc gaaatgcgca cgaaaatggc ggaggaaggg gagcgcggaa
     7081 acaagcattt cagccatttc agccccgaaa ccaatttgtt cgaagagctc gagaggctgt
     7141 ttcgcgacta cggtgaatcc ggcaccggaa cgacccgtca ttacgtccac gacgacgacg
     7201 aggccaagct gtacggcgca gcgccgggca cttttaccga atgggccccg ctgccggaaa
     7261 agtccgatct gctgttttat gaaggcctgc atggcgcggt ggtgaccgac aaggtcaata
     7321 tcgcgcaaca tgccgatctc aagatcggcg ttgtgccggt catcaatctg gagtggatcc
     7381 agaagctgca ccgcgaccgg agcgaccgcg gctacaccca ggaggcggtg accgacacga
     7441 tcctgcggcg gatgccggac tacgtgaatt acatctgtcc acagttcgcc gagaccgaca
     7501 tcaatttcca gcgcgtgccg acggtcgata cctcaaaccc gttcatcgcg cgctggattc
     7561 cgacgccgga cgaatcgatg gtggtgatcc ggctcaggaa cccgcgtggc atcgattttc
     7621 cctatctgtt gtcgatgatt ccgaacagct tcatgtcgcg cgccaattcg atcgtgattc
     7681 acggatcgaa actcgatctg gcgatgcagc tcattctcac cccgctgatt ctgcaactga
     7741 tggaacgcaa gaggcgcacc gtatgagtat tcatgctctt tcttcattcg ccggttgttc
     7801 ggatggctcg catcaagaat tggcaaatgc gattcgcttt ctggcgatcg atgcggtcga
     7861 aaaagcgaag tcgggtcatc cgggtatgcc gatgggcatg gctgacgtag ccacggtgct
     7921 gttttcgcgt ttcctcaaat tcgaccctgc cgatccggag tggccggatc gcgaccggtt
     7981 tgtgctttct gccggtcacg gctcgatgct gctgtatgcg ctgttacacc tgacgggata
     8041 cgagggtgtt acatcagacg agctcaaggc gttcaggcag tggggatcga agacgcccgg
     8101 tcatcccgag tatggccata ccccgggcgt tgagaccacc accggccccc tggggcaggg
     8161 catcgccacg gcagtcggca tggcgctggc agagcgcttg ttgaacgcgc ggttcggcga
     8221 cgatctcgtc gatcactata catatgtgat cgccggtgat ggttgcctga tggaaggcct
     8281 cagccacgaa gcaatttcgc tggccgggca tctcgggctt ggcaagctga tcgtgctgtt
     8341 cgacgacaac cagatttcga tcgacggatc gacatcgctg tcctgttcgg atgaccagct
     8401 tgcgcgcttc aaggcatcgg gctggtcggc tgaccgtatc gatgggcacg atccgcaagc
     8461 cattgcaaca gcgatcgaac gcgcccggag caatgacctt ccatcgttga tcgcgtgccg
     8521 gacggtcatc ggcttcggcg caccgaaccg ccagggcacc gaaaaagccc acggcgcgcc
     8581 attcggtgcc gaggaggtcg caaagacacg cgttgcgctg gactggccat acgaaccttt
     8641 ccagcttcca gaacacatcg tcgcgcaatg gcacaagatt ggcgcaaggg ggcaggccgc
     8701 ccgcctgagc tggatggggc ggacaaggcg tctcaattcg gtcccgcgct cgccgctcca
     8761 tgacgcgctg aaccggaaac taccctgtga atacgctgat gcgattgatc agatctgcac
     8821 ccgtttcggc gctgagcgtc ccaccatagc cacccggcaa gcgtcccagc tggtaatcga
     8881 cgcgatcgcg gaagccttgc cgaatttatt ggggggctcg gctgatctaa cccactccaa
     8941 cctgacgcgc gccaagacac agcgtccggt gcgggccgat tccttcgaag gcaactatat
     9001 ccactatggc gtccgcgaac atgccatggc ggcggcaatg aatggcatcg cgttgcatgg
     9061 cggcttcatt ccctacggcg gcacgtttct taccttcgcc gattacagcc gcccggcgat
     9121 ccggctggcc gccctgatgg gcatcagggt cgtccacgtg atgacgcacg actccatcgg
     9181 attgggcgaa gacggcccga cccatcagcc ggtcgagcat ctcgcctcgc tgcgggcgat
     9241 tcccaatctg ctggtgttcc ggcccggcga cgctgtcgaa actgccgagg cgtgggactg
     9301 tgcgctacgg tcggaagcca gtccttcggt gctgtgcctg tcgcggcagg cgctgccggc
     9361 gtttcgtgaa gttaacgacg agaccaacct ggttgcgttt ggcgcctatg tcgtcgttga
     9421 gccggaggac ggccgcgatg tgacgctgat cgcaacgggt tccgaggttt cgatcgcgat
     9481 cgaggccgca aagctgcttg caaaggacag cgtccgcgct gctgtcgtct cggcgccgtg
     9541 cttcgatctg tttcgccagc aatcgcgcga ataccgcacc gcggttctgg gacgcgttcc
     9601 cagaataggc gtcgaggccg cgatcgaggg cgactgggcg cgctggcttg gcgacggcgg
     9661 cgaattcgtc ggcatgaccg gcttcggcgc atccgctccg gcggaggtgc tgtatcgcga
     9721 attcggcatc accgcgcagg ccgtcgctac cgcggcgctg cattgcctgg tgcggtcgaa
     9781 aatgccggca acccacccat gaagtctaga cgcgacaaag catgacacgg agacaccgat
     9841 ggca
//
LOCUS       DATNOI010000588        17351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1630708,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000588 DATNOI010000000
VERSION     DATNOI010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17351
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630708"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..819)
                     /locus_tag="VN968_23760"
     CDS             complement(268..819)
                     /locus_tag="VN968_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32284.1"
                     /translation="MARAKRNKPHHPAKVHDRRSRDLPRNAEVAAVEVDDPFGLEPGD
                     KIVTLRSIRNDPLGRLHTHRQIDEAQYQGGRAFQSDWEKAERGPQAVDPTREYVDGGQ
                     RREPITEGQLKAVLRLNRAERELGADGSALVHEVLILGMTMEQIGQRRGVRGQRWIDY
                     FARRFRECLDRLALIYGFATERR"
     gene            complement(905..1054)
                     /locus_tag="VN968_23765"
     CDS             complement(905..1054)
                     /locus_tag="VN968_23765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32285.1"
                     /translation="MKRLPRRHRIAHLRALIPQQPAHSIRREELVALLRDEMLEGPGK
                     ETRAM"
     gene            complement(1096..1362)
                     /locus_tag="VN968_23770"
     CDS             complement(1096..1362)
                     /locus_tag="VN968_23770"
                     /inference="COORDINATES: protein motif:HMM:NF019370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HXL32286.1"
                     /translation="MPALESVKPVKLRCVRIDPRYLSLVDLEPGDCRFPYGGDKEGET
                     ITFCGHPRRQGTSYCAAHFYLTRGPDAAPERPGGTISLRLVEAA"
     gene            1467..2120
                     /locus_tag="VN968_23775"
     CDS             1467..2120
                     /locus_tag="VN968_23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HXL32287.1"
                     /translation="MLDIRMIERGLEKPGKTKGGLAMAMGVRPGAVSEILAEIRLIKA
                     TEIAPIIEYLELNSVPIMGRVGAGAVIEPEHEQVPPEGLGEVELPFPIAEETIAFEVV
                     GDSMLPKYESGDIIVVYKEQRHPPTSFYGEEAAVRLTTGERYLKTIERGKSKTQVNLT
                     SFNAKPINGVKLEWIGEICVTLPRGQIERLRTKAAGRARKGARAHAGAPPREMKATK"
     gene            2279..2554
                     /locus_tag="VN968_23780"
     CDS             2279..2554
                     /locus_tag="VN968_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32288.1"
                     /translation="MQYFVVMIDYGRRGREAIVDPEITRRQVVSRVASGEYKNISFIH
                     EIADSAVNDVTAEIMAETALPEIPIIGAELQADSFDHARDLAKHEAV"
     gene            complement(2956..3672)
                     /locus_tag="VN968_23785"
     CDS             complement(2956..3672)
                     /locus_tag="VN968_23785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32289.1"
                     /translation="MSAQPVVQVEDLDVYCGTSQILFGVDLKVQQGETMALLGRNGAG
                     KSTTMKAIMGLAPARRGKVMLRGRVISGLKPHHIARAGLGFVPEDRQIFPDHTVEDNL
                     VIGQKKGAEGQEEWSIRRVYEVFPLLEPLRHRIAGRLSGGEQQMLAIARTLMGNPALL
                     LLDEPSEGLAPIIVQRIGELLRQLRGTGATVLIAEQNMHFCLGLASHATVIDKGQIVY
                     TATIEDLKANDNIRQRYLAL"
     gene            complement(3669..4427)
                     /locus_tag="VN968_23790"
     CDS             complement(3669..4427)
                     /locus_tag="VN968_23790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32290.1"
                     /translation="MLEVKHLSKSFGGVKAADDITLSFAEGSLTAVIGPNGAGKSTFF
                     NLITGALRPDSGHILLNGVDMAGRSPPDIVRHGIGRAFQVARIFPSLTVHETMLAAVG
                     ADQRRASVLHRRFPLAETRDRAEHAMEVLGLTGKRDRTAATLSHGDQKLLDIALALVL
                     DPQVLLLDEPTAGMGTEERWRMIDQVHELWERQKITVVFIEHDMDIVFKIAPMVVVLC
                     YGRILATGTPEAIRQNPAVIEAYLGTERHTEAAV"
     gene            complement(4430..5473)
                     /locus_tag="VN968_23795"
     CDS             complement(4430..5473)
                     /locus_tag="VN968_23795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL32291.1"
                     /translation="MTELQAERARKRTIESSAVPRVETRSKRLRYIDVLIAVAAFAVL
                     AALPFLFSSKALIDFAIRCAAFGLFATSLNLLVGYTGMISFGHGMFFGLGAYGFGLIM
                     QRTGVPIPVAFIGTLLITTLVAAVIGAICVRLKEIYFAFVTLAFQMLIHSTILSWTSL
                     TGGDQGLRGGIPRPVFLGIDLSNQTHLYIASCTLLVGGLLLMRQIAQSPFGYTLRMIR
                     DNASRASFIGIDVWRAKLTIFVLAALFASTGGIIMSLFVSGAYPEFAYWTISGEGIFI
                     NMLGGITTFLGPMVGTVLLLILNDTVTRLTEYYGIVLGIVILFFAIGLRKGLMDFMVE
                     WYEQRRDGAKERA"
     gene            complement(5470..6372)
                     /locus_tag="VN968_23800"
     CDS             complement(5470..6372)
                     /locus_tag="VN968_23800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL32292.1"
                     /translation="MDLDALTGCLASSACLVTQTTSGFIIGMLLFLVAVGLTLIFGVL
                     KVVNFSHGAFYMFGAYFAMTAYQVTGSFALAMFCGASGTAILGLVFERVFMSRVYGGD
                     VLMQLLVCYAFVLIFDDVVRLVWGPEFRSMGMPSAFQVPPLFIAGGVVPPYYLLLIGV
                     ALAVAAILGVGLARTKVGKVIRAAAHNPGMVSALGINTGLIYGGVFALGCMLAGLAGA
                     LAAPVRSLTPGMGFSVLIESFIVTVIGGMGSIFGALIGALLIGMIRSYGSLGFPLFTD
                     GMMYLFMVIVLVARPTGLFGKEVA"
     gene            complement(6462..7721)
                     /locus_tag="VN968_23805"
     CDS             complement(6462..7721)
                     /locus_tag="VN968_23805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32293.1"
                     /translation="MTETSRPGVSRRSTLALMGASAVTFATPWVARAQAKTIKIGMPT
                     ILSGRVAQLGTSSRNGVMIEVDKINAAGGLAGRQIEMVIRDSKGAPQEAARVARELVN
                     TDGCEILVDAEASSGAFAVHEVARDLGVFCIHTNSETSSLTADPKQHIPNAFRTARQG
                     VHDSIVGGGYAAAIAKAKGLKKWATCSPDYAYGRDTTGEFVYYLKQFAPDVEIISDAW
                     PKLFQPDYTEVVTKILQAKPQALYSALWGGDLTSYIDQANIYALFSQMEVFAVNMADY
                     TALTVVKNLPKGIHSGNRYIKTYPATAENAAWGDAYKAKYNEYPTNWSWENATAVMLI
                     AEASRKANSADGKKLADAMRGLTIKSPFGADGTVTMRAEDQTLIGYAIGWGTTIPNEP
                     YVPEVKSGDWKQILALEAEWKKTKGYT"
     gene            7885..9378
                     /locus_tag="VN968_23810"
     CDS             7885..9378
                     /locus_tag="VN968_23810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXL32294.1"
                     /translation="MDPSNFIAFHARQTPERCALKYRGEEITYAAFHARIQTVGGWLA
                     SRGIGPDDVVAVLMKNSAAFLDIAFAVSRIGAIFLPINFRLASDEIAYIVENSGARLL
                     IADEEFRASAAGPAPIVLVDVTAQSNATRLGPEAKPAKAHRRLPSDLTRLMYTSGTTD
                     RPKGVMLTYDNIHWKSVDHVLALGLSRDTRLLVAGPLYHVGALDLPGIAVLWQGGMLS
                     ILRDFDAAQCLAAIEADRLDGAWLAPVMTTSILTLIDRERYDVSSLRWAIGGGEKTPE
                     ARIRAFSQYFTNARYIDAYGLTETCGGDTFMEPGLEITKIGSTGRAVAHVEIEIRDDM
                     GHRVVPGENGEICLRGPKVTRGYWKDPEETAAAFFGDWFRSGDVGYLDEDGFLYLTDR
                     KKDMIISGGENIASSEVERVIYELPQVREVAVIGMPDHRWGERPIAVVVLADSAALEL
                     ADLTAHCRQRLASFKVPKQLIIRDNLPRNPSGKVLKRVLRAELEANA"
     gene            9375..10034
                     /locus_tag="VN968_23815"
     CDS             9375..10034
                     /locus_tag="VN968_23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL32295.1"
                     /translation="MTQVPEAAAGKLTKLNRVQRNAWTKRKIFDAATKIVGKYGYAEA
                     SVARITEEAGVAQGTFYNHFENRQELLDQLLPMIGTEMVRFIRERTGTAHAARQEIER
                     FGAFFDFIREVPEFLRILNEAEFFAPIGYQKHLDNISTAYVRILRRARHAGAIDNFSD
                     EEFEAIVHMFMGARGYLSRRYSYSGGTVTAVPDHVISAYEKLITRGLFTPRSEGDNHD
                     R"
     gene            10024..10797
                     /locus_tag="VN968_23820"
     CDS             10024..10797
                     /locus_tag="VN968_23820"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL32296.1"
                     /translation="MIAEGIVLVTGGSRGIGAATATLLAQQGRRVVIADVGPEALIGT
                     QTILWPNRFDVASETEVMSGIADIETAHGPITGLVNAAGVFGNMHSVERVRMDNWDRE
                     INIDLRGTFLVARSVGVRMAKRRHGAIVNVASVAGMTSGPIHAYTAAKAGVIQITQTL
                     AAEWGRAGVRVNAVSPGFTRTAALEAGISSGALDQNRLARNTAMNRLVEPTEVAHAIA
                     WLLSPVSSGVTGINLPVDAGFIAGSTWDAYGGLREAPEV"
     gene            10809..12716
                     /locus_tag="VN968_23825"
     CDS             10809..12716
                     /locus_tag="VN968_23825"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL32297.1"
                     /translation="MNIEVPRKKLDLSSAIAEGDIRVLLMVLVHMTGDERWLQPPYKP
                     QRDVRLIPDPEAGVPKEIQDEIRTAVLKLFVDGGPSPAITDPGDELMLKMMRACLGEN
                     VAPEYAPLMREEMGFIPRDARWTRRPSGDALMKQHVLIVGAGVCAIALGVALGRLGIP
                     YTIVEKNDELGGTWYVNRYPGCGVDTPNHSYSFSFGARNNWTRYFAQREELLDYLKKV
                     ALEHDVRSHIRFNTRLTSSRWDETRRRWVSTLETGNGVEDFESAVLVSAIGQLNDPSP
                     AHFKGEEDFSGRMFHSALWSNDIKLDAKRVAVIGTGATAMQLVPSIADRVASVTVYQR
                     SAQWSRPVTGYSDPISEGAQWLLAHLPFYVQWYRFNMFWRYGDGLLPFLRKDPNWPYP
                     DRAVNKGNDRHRQELTDFILTELKDRPDLIEKCVPTYPPYGKRILLDNGWFRTLTKPH
                     VELVTEPIGHFARDGIVTSDGKLRPADVIVISTGFKVTQMAARLNISGNGKKLADAWA
                     DDNPTAYLGLTVPDFPNFFCMLGPNAGPAHGGSVIFQSECQSRYITACLVNMIERGIA
                     AIDVRQDIHDRYVREVDAEHEQMIWTHPGMTTYYRNKQGRVFSAMPWRFVDYWRMTHD
                     PDLSQYRQTKA"
     gene            12740..13369
                     /locus_tag="VN968_23830"
     CDS             12740..13369
                     /locus_tag="VN968_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HXL32298.1"
                     /translation="MTRPRVRIYTDYKSPYAFVANKRLFELEENHGVELEWLPYTLRI
                     AEFMGKVEERTPHFWRKVRYAYMDARRYANTQGLTMKGPRRIYDAFYSSAGMLFAQRH
                     GLFRPYHDTVFRRFWSHDLEIDELSGISDVIASVGGSATDFEAYVNGPARAEHDRIID
                     EAEALGVFGVPTMVFNGELFWGGDRIDMLIERIKKPETIAAALGSRHRA"
     gene            complement(13403..14200)
                     /locus_tag="VN968_23835"
     CDS             complement(13403..14200)
                     /locus_tag="VN968_23835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32299.1"
                     /translation="MRSRNPAPGSVVPEDKATAFVCDLLEDLADEWAAKPLFLYRWCD
                     PEDQAYVSRWAAEEWSVSDAVAGSAEEIEHFRVRQISRMPILGATAENKPLLEESYRR
                     ILAAFEPHVGMTKYLFGSRPSLADFAWFGQLSELATDPTPMRIMREKAPFTDHWVRRL
                     DDASGVDGKWYPREQALSGMAEALLGIAGELYLPFLAANAQAFAKGVERLEISVWGLP
                     YALSPFKYQVKCLDLIRGKFAALDAEHRTALRPVLERTGCWEHLVAK"
     gene            14682..15494
                     /locus_tag="VN968_23840"
     CDS             14682..15494
                     /locus_tag="VN968_23840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL32300.1"
                     /translation="MKGPEDDSGLTDRVAIVSGGGAAGDGIGNGRAAAILLARAGTKV
                     LVADLDLRLAERTVEMIQAEGGTAAALSGDITKESDCMRLVEAAVDRWGRLDFLDNNV
                     GIGSRGSVVDEKPEEYRRVMQINVETMFLLSKYAIPAMIKTAGGGSIVNISSISALRP
                     RGLTTYTTSKAAVIGLTQAMAVDHGGDNIRVNCICPGPMYTPMVYARGMSDAARTQRA
                     NASLLKTEGTGWDVGHAVKFLLSNYARYITGQVLVVDGGVTLQGPERDSRDH"
     gene            15522..16076
                     /locus_tag="VN968_23845"
     CDS             15522..16076
                     /locus_tag="VN968_23845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXL32301.1"
                     /translation="MARLPYLEADQVAPEYRDMLKRNTNLHKLLVNSPDMARAFNGIG
                     GYIRFKSKLDPRLRELAILQVGWLEKSEYEFTHHVSIGKEFGVTDEDIRGLIAETEGK
                     PSQLEPLAKAIVKGAREMVRELAMSDSTFAEIKQELSDEHMTDLVLTIAFYCAVVRVL
                     ATMKIDNEPTYKEVLQQYPMPGVK"
     gene            16078..16878
                     /locus_tag="VN968_23850"
     CDS             16078..16878
                     /locus_tag="VN968_23850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL32302.1"
                     /translation="MRLKDRVAIVVGAGQSPGEGIGNGRATALTFAREGAKVLCVDHN
                     IESAQETVDMIDAKGGTAVAYKADVTKEAEIKAMAADAHTRWGRIDILHNNVGVSLAG
                     GDAELLDLTEEALDRCVAINLKSCIFAAKHVIPIMRRQNSGAIINISSMAVITTYPYV
                     AYKATKSAMVAFTEQLAYQNAQYGIRANAILPGLMNTPMAVDTRAREFKKSRAEVEAE
                     RDAKVPLRKKMGTGWDVANAALFLASDEASFITGVTLPVDGGASVRRG"
     gene            16885..>17351
                     /locus_tag="VN968_23855"
     CDS             16885..>17351
                     /locus_tag="VN968_23855"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL32303.1"
                     /translation="MAASHETKSPPGAAPALEYDAIIIGAGMSGMYQLYRLRELGMRV
                     QVFEAGTGVGGTWYWNRYPGARFDSESYSYGYSFSKELLQEWDWSEHFAGQPETLRYL
                     NLVADKFDLRRDIQFRSRVAAATYQEDTHSWNVTLADGSHFRSRFLITAIGPLS"
BASE COUNT         3573 a         5449 c         5127 g         3202 t
ORIGIN      
        1 tccctccaca tacgccgcaa accgtgcttc actgtcgccg acatgcttga gattcatctg
       61 gccctccacg tgccaaaatc tgaatccctt caattatttg attctcggcc gcaacggctg
      121 cgaccttctg actcagtacg agtaaccagc ggctcggcgc ccgcgggtcg gcaagtggcg
      181 tggcggatca ttcagaattc gtccggcggc cggccggaca acccgtcgac gatggcgcct
      241 tacgggttac tgacaaactg gaacaagtta ccgccgctcc gtcgcgaacc cataaatcaa
      301 agccagccga tccaggcact cgcggaatcg ccgcgcgaaa tagtcgatcc agcgctggcc
      361 gcgcactccc cgccgctgcc cgatctgctc catggtcatg ccgaggatta atacctcgtg
      421 caccagcgcc gatccgtcag cgccgagttc acgctcggct cggttcagcc gcagcacggc
      481 cttgagctga ccttccgtga tcggctcccg cctctgcccg ccatcgacat attccctggt
      541 gggatcgaca gcctgaggtc cgcgctcggc tttctcccaa tcgctctgga atgcccgccc
      601 gccctggtat tgcgcctcgt cgatctgacg atgcgtgtgc agccggccta agggatcgtt
      661 gcggatcgag cgcagcgtga cgatcttgtc tcccggctcc aggccgaagg gatcgtccac
      721 ttcgacggca gccacttctg cgttgcgcgg caaatcccgg gatcgccggt catgtacctt
      781 cgcaggatga tgcggcttgt tgcgtttggc ccgtgccata cctgatcgct ccgttaggtg
      841 gtggaatttg aatgttctgg tcacgccctt cgctcttgca attctcaggg cgagatgggc
      901 cggttcacat tgcgcgcgtt tctttgccgg gcccctccaa catttcatcg cgcagcaatg
      961 caaccagttc ttcccggcgg atcgaatgtg ccggttgctg cgggatcagc gcgcgcagat
     1021 gcgcgatgcg atggcggcgc ggcaaccgct tcatccggat aatcatgatc gccagcgtcg
     1081 gtttcggtcc gcgcttcatg ccgcctccac cagcctgagg gagatggtgc cgccaggtcg
     1141 ttccggcgcg gcgtcgggcc cgcgcgtcag gtagaaatgc gcggcacaat agctcgtgcc
     1201 ctggcgccgc gggtgaccgc agaaggtgat ggtctcgccc tccttgtcgc caccataagg
     1261 aaaacggcaa tcgccgggtt caagatcaac cagcgaaaga tagcgcggat caatccgcac
     1321 gcagcggagc ttgaccggct taacactttc gagcgccggc atcggtcgac tggattcgga
     1381 agtcaagcgc tcgcgtcgct tgtgcccaag tccaatttct gaaaatcgga attgatttct
     1441 tttctgaaat tcagaaatat ggtggcatgt tggatatcag aatgatcgag cggggacttg
     1501 aaaagccggg caagaccaag ggtggtctgg caatggccat gggcgtccgc cccggcgcgg
     1561 tttccgagat attggccgaa atccgcctga tcaaggctac ggagattgcg cccatcatcg
     1621 agtatctcga gctgaattcg gtgccgatca tgggacgtgt cggcgcaggg gcggtcatcg
     1681 aaccggaaca cgagcaggtg ccgccggagg gcctcggcga agtcgagttg ccctttccca
     1741 tcgcggagga aaccatcgcc tttgaagtgg tcggcgattc catgctgccg aaatacgaaa
     1801 gcggcgacat tattgtggtc tacaaggaac agcgccatcc gccgacgagc ttctacgggg
     1861 aggaagcggc agtcaggctg acgaccggcg agcgttattt gaaaaccatc gagcgcggca
     1921 aatccaaaac gcaggtcaac ctcacgagct tcaacgccaa gccgatcaac ggggtcaaac
     1981 tcgaatggat tggagagatc tgcgtcaccc tgccaagagg ccagatcgag cggctgcgga
     2041 ccaaggccgc gggccgggcg cgcaagggcg ccagggcgca tgccggggct cccccgagag
     2101 aaatgaaggc gactaaatga gcatgcgcgc caagtgattc ccgccttcag ccgggcgctg
     2161 ccttgccgat cgaatccatt attctgattt tccgaatttt tggacttgac taacttccga
     2221 ttttccgaaa tactcgcttt cgtccgccgg ccgtgtacgc gcgggcaaag cgagcgccat
     2281 gcaatatttc gttgtgatga tcgattacgg acggcgcgga cgcgaggcca ttgtcgaccc
     2341 tgaaatcacg cggcggcagg ttgtctcgcg cgtcgcctcc ggcgaataca aaaacatcag
     2401 cttcattcac gagatcgccg attctgcggt gaatgacgtc acggccgaga tcatggccga
     2461 gacagcactt cccgaaatcc cgataattgg cgccgaactg caggccgata gcttcgacca
     2521 tgcccgcgat ctcgcaaagc acgaggcggt atgatggata tgcgacccgg attggttcgc
     2581 agggcgcatc aagcagagcg tctgacgccc tcccccatta tccttcccgc cgaagcgata
     2641 gccgtgacgc tcctgatcct tgtcgtggcg cttggcctga cgatccggag cggcatgacg
     2701 tcggcggcgc acaatgatgg ggcgcatgcg ggttgcgggt tgaacgctcg gtagcggtcg
     2761 agagatcgcg tttgtgtgaa ttgtccgttc cggtccagcg tggaatggtc cagcggtagc
     2821 ccccgaaaaa tcggcaagcc atgacgcggc acgatttata gggacttctt gcggctggcg
     2881 aacgattgtc gcgttacacg atgctatttg caccattcaa tggcagcttg gaaaaggaat
     2941 gagtccgtct tgcggctaca gcgcgagata gcgctggcga atgttatcgt tggctttgag
     3001 atcctcgatt gtcgcggtgt agacgatctg gcctttgtcg atgacagtgg cgtggctggc
     3061 gagcccgagg cagaaatgca tgttctgctc cgcgatcaaa accgtagcac ccgtgccacg
     3121 cagttgccgc agcaactcgc cgatccgctg cacgatgatc ggtgccagcc cctcgctcgg
     3181 ctcgtcgagc agcaacagcg ccggattgcc cattagcgtc cgcgcgatcg cgagcatctg
     3241 ctgttcgcca cccgacagcc ggcccgcgat ccggtgtcga agcggctcca gcagtgggaa
     3301 cacttcatag acacgccgga tcgaccattc ttcctgtcct tcggcgcctt tcttctggcc
     3361 gatgacgaga ttgtcctcca cggtatgatc gggaaagatc tgccggtcct cgggcacgaa
     3421 gccaagcccg gcgcgggcga tgtgatgtgg cttgagcccg gatatgacac gaccccgcag
     3481 catcactttt ccgcgtcgtg ccggcgcgag acccatgatg gctttcatgg tggtcgattt
     3541 gccggccccg ttgcggccga gcagcgccat ggtttcaccc tgctgcacct tcagatcgac
     3601 accgaacagg atctggctgg tgccgcaata aacatcgaga tcctcgacct gaacgacggg
     3661 ctgcgcactc ataccgcggc ctcggtgtga cgttcggtgc ctagatacgc ctcgatgaca
     3721 gcggggttct ggcggatcgc ttcgggcgtg ccggtcgcga gaatccggcc atagcacagc
     3781 accacgacca ttggcgcaat cttgaagacg atgtccatgt cgtgctcgat gaagacgacc
     3841 gtgatcttct gccgctccca gagctcatgg acctgatcga tcatgcgcca gcgctcttcg
     3901 gttcccatgc cggcggtggg ctcgtcaagc agcaatacct ggggatcgag caccagtgcc
     3961 agcgcgatat cgagcagctt ctggtcgcca tgcgaaagtg tagcggctgt ccggtcgcgc
     4021 ttgccggtga ggcccagcac ctccatcgca tgttcggcac ggtcgcgcgt ctcggcgagc
     4081 gggaaccgcc gatgtagcac actggcgcga cgctgatcgg cgcccaccgc ggccagcatc
     4141 gtttcgtgca ccgtgagcga agggaaaata cgcgccacct gaaaggcccg gccgatgccg
     4201 tgacgcacga tatcaggcgg ggaccgcccc gccatatcca caccgttgag caggatgtgg
     4261 ccagagtctg gcctgagcgc acccgtgatc agattgaaga atgtactttt tcctgcgcca
     4321 ttggggccga tcaccgcggt cagcgatccc tccgcgaaac tcaacgtaat atcgtcggcc
     4381 gccttgacgc cgccaaacga tttggagaga tgctttacct ccagcatggc taagcacgct
     4441 ccttcgcacc gtcgcgacgc tgttcgtacc actcgaccat gaaatccatc agacctttgc
     4501 gcaagccgat cgcgaaaaac aggatcacga tgccgagcac gatgccgtaa tactcggtga
     4561 gccgcgtgac ggtgtcgttg aggatcaaca gcagcacggt cccgaccatc gggcccagga
     4621 aggtggtgat accgccgagc atgttgatga agatgccctc gcccgaaatt gtccagtacg
     4681 cgaattcggg ataggcgccc gacacgaaca ggctcatgat gatgccgccg gtagaggcaa
     4741 acagcgccgc cagcacgaag atcgtcagct tggcgcgcca gacgtcgatg ccgatgaagc
     4801 tggctcgact ggcgttgtcg cggatcatgc gcaaagtgta gccgaacggc gattgcgcga
     4861 tctgacgcat cagcagaagt ccgcccacca gcagcgtgca gctcgcaatg tatagatgcg
     4921 tctggttcga caaatcgatg ccgagaaaca ccggacgtgg aataccgccg cgcaaacctt
     4981 gatcgccgcc ggtcagcgaa gtccacgaca atatcgtact gtggatcagc atctgaaacg
     5041 ccagcgtgac aaaggcgaag taaatctcct tcagccgcac gcagatggcg ccgatcaccg
     5101 ccgcgaccag ggttgtgatc agcagcgtgc cgataaaggc gaccgggatc gggacgccgg
     5161 tgcgctgcat gatcagaccg aaaccgtagg cgccgaggcc gaagaacatg ccgtgcccga
     5221 acgaaatcat gccggtgtaa ccgaccagca gattgagtga ggtcgcgaac agaccgaacg
     5281 ccgcgcatcg gatcgcgaaa tcgatcagcg ccttgctgct gaacaaaaat ggcaacgcgg
     5341 ccagcactgc aaaggctgcg acggcaatca gcacatcaat gtaacgcaag cgcttcgatc
     5401 ttgtctcgac gcgcggaacg gccgaagatt ctatggttcg tttacgagcg cgttcggcct
     5461 gcagctcggt catgccactt ccttgccgaa cagtccggtc ggccgggcaa ccagcacgat
     5521 caccatgaac agatacatca tgccgtcggt gaatagcgga aaaccgagcg aaccgtatga
     5581 ccggatcatg ccgatcagga gcgcgccgat cagcgcgccg aagatcgaac ccatgccgcc
     5641 gatcaccgtc acgatgaaag attcgatcag caccgaaaat cccatgcctg gcgtcagcga
     5701 acgcacgggt gcggcgagcg ctccagcaag gccggccagc atgcagccga gcgcaaacac
     5761 gccgccatag atcaagccgg tgttgatgcc aagcgcggac accatgcccg gattatgcgc
     5821 ggcggcccgg atcaccttgc cgactttagt gcgggcaagg ccgacgccga ggatcgctgc
     5881 aaccgcgagc gccacgccaa tcaacagcag atagtatggc ggcacgacgc cgccggcgat
     5941 aaacagcggc ggcacctgga acgcgctggg cattcccatc gaccggaatt ccggtcccca
     6001 caccagccgc accacgtcat caaagatcag gacaaaggca taacagacca gaagctgcat
     6061 cagcacatcg ccgccataga cgcgactcat aaagacccgc tcgaacacca ggccgaggat
     6121 cgcggtgccg gaggcgccac aaaacatcgc cagcgcaaag ctcccggtga cctgataggc
     6181 ggtcatcgcg aaataggcgc cgaacatata gaacgcgcca tggctgaaat tcacgacctt
     6241 gagcacgccg aagatcagcg tcagcccgac cgccacgaga aacagcaaca ttccaataat
     6301 gaagccgctg gtggtttgcg tcaccagaca ggccgagctg gcaagacagc cggtgagcgc
     6361 gtcgaggtcc aaggaagatg tccgttacgg catgccgggt tatctcagcg gaaagaacgt
     6421 tcggcggtag gccgcggcag cctccgcgtt ccactgacgt gtcaggtgta gcccttggtc
     6481 ttcttccact cggcttcaag cgccaaaatc tgcttccagt cgccgctctt gacctccggg
     6541 acataaggct cattaggaat cgtggtgccc cagccgattg catagccgat cagagtctga
     6601 tcctcggccc gcatcgtcac ggtgccgtcg gcgccgaacg gcgatttgat ggtcagacca
     6661 cgcatcgcgt ccgctagctt tttgccgtcc gctgaattgg ccttcctcga cgcctctgcg
     6721 atcagcatca ccgccgtcgc attctcccac gaccagttgg tgggatactc attgtacttc
     6781 gccttgtagg catcgcccca cgcagcgttc tccgccgtcg ctggataggt cttgatgtag
     6841 cgatttccgg agtggatgcc cttcggcaga ttcttgacca cggtcaacgc cgtataatcg
     6901 gccatgttga cggcgaacac ctccatctgg ctgaacagcg cgtagatgtt ggcctgatcg
     6961 atgtaggaag tcagatcgcc gccccacagc gcggaataca gcgcctgggg tttcgcctgc
     7021 aaaatcttgg tcacgacctc ggtgtaatca ggctgaaaca gtttcggcca ggcgtcgctg
     7081 atgatttcga cgtcgggcgc gaactgcttg aggtagtaga caaactcacc cgtcgtatcg
     7141 cggccgtagg cataatccgg cgaacacgtc gcccatttct tcagtccctt ggccttggcg
     7201 atggccgcgg catagccacc gccgacgatc gaatcatgaa cgccctggcg cgcggtgcgg
     7261 aacgcgttgg ggatgtgctg ctttggatcg gctgtcagcg aggaggtttc cgagttggtg
     7321 tgaatacaaa aaacgccgag atcgcgcgcg acctcgtgca ccgcgaacgc tcccgaggac
     7381 gcctctgcat cgacaagaat ttcgcaccca tcagtattga ccagttcgcg tgcgacgcgg
     7441 gcggcttcct gcggcgcccc cttggaatcg cggatcacca tttcgatctg ccggccggcg
     7501 agcccgcccg cggcattgat cttgtcgacc tcgatcatca cgccgttgcg cgacgaggtt
     7561 ccgagctgcg ccacgcggcc cgacagaatc gtcggcattc caattttgat cgtcttggct
     7621 tgcgcgcgcg ccacccatgg cgttgcgaat gtcaccgcgc tggcgcccat cagcgccagc
     7681 gtcgaccgcc ggcttacgcc cggcctgctg gtctctgtca tcccgcctcc tcgacgtctg
     7741 tgcgtccctc aaaacccctg ccggagatcg tgccgtctcc atgccgttag aatttcagag
     7801 aaaacggggt gacgtcaagg caattatgaa ttacgagtca taattcatat tgcgcgcaag
     7861 ttaggcagtt ccgggcgagt aatgatggat ccatcgaact tcatcgcatt tcacgcccgg
     7921 caaacgccgg agcgctgtgc gctgaaatat cgcggcgaag agatcaccta cgcggccttc
     7981 cacgccagga ttcaaaccgt cggggggtgg ctggcgtcgc gcgggattgg tcctgatgac
     8041 gtcgtcgccg tgctgatgaa aaacagcgcg gcctttctcg atatcgcttt cgccgtaagc
     8101 cgcatcggcg cgatctttct gccgatcaat ttccggctgg cgtcggacga aatcgcctac
     8161 atcgtcgaaa attccggcgc gcgcctgctc atcgccgacg aggaatttcg cgcgagcgcg
     8221 gcgggacctg ctcccatcgt cctggtcgat gtcaccgcgc aatccaacgc tacgcggctt
     8281 gggcccgaag ccaagcccgc aaaggcgcat cgccggcttc cgtccgacct gacgcggctg
     8341 atgtacacct ccggcaccac cgatcggcca aaaggcgtga tgctgactta cgacaacatc
     8401 cactggaaat cggtggatca tgtactggcg ctcggcctca gccgcgatac aaggctgctg
     8461 gtcgccggtc cgctctatca tgtcggcgca ctcgatcttc ccggtatcgc ggtgctgtgg
     8521 cagggcggaa tgctctctat tctgcgtgat ttcgacgccg cgcagtgcct ggctgcgatc
     8581 gaagccgacc ggctcgacgg agcatggctg gcgcccgtca tgacgacatc catcctcacc
     8641 ctcatcgatc gcgaacgcta tgacgtctcc agcctgcgat gggcgatcgg cggcggtgaa
     8701 aaaactcccg aggcgcgcat tcgcgccttc tcgcaatatt tcaccaatgc ccgctacatc
     8761 gacgcctacg gcctgacgga aacctgtggt ggcgacactt tcatggagcc tgggctggaa
     8821 attacaaaga tcgggtcgac cggccgcgcc gtcgcccatg tcgaaatcga aatccgcgac
     8881 gatatgggcc atcgcgtcgt ccccggcgag aacggcgaaa tctgcctgcg cggcccaaag
     8941 gtcacgcgcg gctattggaa ggatcctgag gaaaccgcgg cggcgttttt cggcgactgg
     9001 tttcgtagcg gggacgtcgg ctatctcgat gaggacggct tcctttatct caccgaccgc
     9061 aagaaggaca tgatcatttc cggcggcgag aatatcgcgt cgtccgaagt cgagcgcgtc
     9121 atctacgaac tgccgcaggt gcgcgaagtc gccgtgatcg gtatgcccga ccatcgctgg
     9181 ggcgaacggc ccattgcggt cgtggtgctg gcggacagcg cggcgctgga actagctgac
     9241 ctgacggcgc attgccgcca acgcctggcg tcattcaagg tgccgaaaca actgatcatt
     9301 cgcgacaacc tgccacgcaa tccctcgggc aaggtactca agcgcgtgct gcgcgccgaa
     9361 ttagaggcca acgcatgacg caagtacccg aagctgcggc tggaaagctg acaaaactca
     9421 atcgcgtcca gcgcaatgca tggaccaagc gcaagatttt cgacgccgcg accaaaatcg
     9481 tcggcaaata tggttacgcc gaagcctccg tcgcccgcat caccgaagag gccggcgtcg
     9541 cgcagggcac gttttacaat cattttgaga atcgtcagga gttgctggat caattgttgc
     9601 ccatgatcgg cacagaaatg gtccgtttca tccgcgagcg gaccggtacg gcgcacgcgg
     9661 cgcggcagga aatcgagcga tttggcgctt tcttcgattt catccgtgag gtcccggaat
     9721 tcctgcgcat cctcaacgag gccgagtttt ttgcaccgat cggctaccag aagcacctcg
     9781 acaatatctc gacggcctat gtccgaatcc tgcggcgcgc ccggcatgcc ggcgcgatcg
     9841 acaatttcag cgacgaggaa ttcgaggcca tcgtgcacat gttcatgggc gcgcgcggct
     9901 acctcagccg ccgttattcc tattccggcg gcaccgtcac cgcggtgccg gatcatgtga
     9961 tctcggccta cgagaagctg attaccagag gattgttcac tccgcggagc gaaggcgaca
    10021 atcatgatcg ctgaaggcat tgttctggta accggtggca gccgcggcat cggtgccgca
    10081 accgcgaccc tcctcgcgca gcagggacgg cgcgtcgtaa ttgccgatgt cgggcccgaa
    10141 gctctcatcg ggacgcagac gatcctgtgg cccaataggt tcgacgttgc cagcgagacc
    10201 gaggtcatga gcggcatagc ggacatcgag accgctcacg gtccgatcac tggcctcgtc
    10261 aatgcggccg gcgtgttcgg caacatgcat tcggtcgaac gcgtgcgcat ggacaattgg
    10321 gatcgcgaaa tcaatattga tttgcggggc acatttctgg tggcgcgcag cgtcggcgtg
    10381 cgaatggcca aacgacgcca tggcgccatc gtcaacgtcg cctcggtcgc gggaatgaca
    10441 tccggcccga tccacgccta taccgcggcg aaggccggcg tcattcagat aacgcagacg
    10501 ctcgcggcgg aatggggacg cgccggcgtg cgggtcaacg ccgtgtcacc cggatttacg
    10561 cgcactgcgg cactcgaggc gggaatatct tcgggcgcgc tggatcagaa ccgcctcgcc
    10621 cgcaacaccg cgatgaaccg gctggtggaa ccaacagagg ttgcccacgc catcgcgtgg
    10681 ctgctctcgc ccgtaagcag cggcgtgacc ggaatcaatc taccagttga cgctggattt
    10741 atcgccggct cgacctggga cgcctatggc ggattgcgcg aagcaccgga agtataggac
    10801 ggaagatcat gaacattgaa gtgccgcgca agaaactcga tctgtcttcg gccatcgccg
    10861 agggcgatat ccgcgttcta ttgatggtcc tggtccacat gaccggcgat gagcgctggc
    10921 tgcagcctcc ctacaagccc cagcgcgacg tccgcctgat ccccgatccg gaagccgggg
    10981 tgcccaagga aatccaggat gaaatccgca ccgccgtcct caaactcttc gtggatggcg
    11041 ggcccagccc tgcaatcacc gaccccggcg acgagttgat gctgaagatg atgcgcgcgt
    11101 gcctcggcga gaacgtcgca ccggaatatg cgccgctcat gcgtgaggaa atgggattca
    11161 ttccacgcga cgcgcgctgg accagacgtc cgtcgggcga cgccttgatg aagcaacatg
    11221 tcctgatcgt cggcgcgggc gtctgcgcca tcgcgctcgg cgtcgcgctc ggccggctcg
    11281 gcatccccta caccatcgtc gagaaaaacg acgaactcgg cggcacctgg tacgtcaacc
    11341 ggtatcccgg ctgcggcgtc gacacgccca atcattccta ctcattttcc ttcggcgcgc
    11401 gcaacaactg gacccgctat tttgcgcagc gcgaagaact gctggattat ctcaagaagg
    11461 ttgcgctcga acatgacgtc cgcagccaca tccgtttcaa cacgcggttg acatcttcgc
    11521 gctgggacga gaccagacgt cgctgggttt cgacgctgga aaccggcaat ggcgtggaag
    11581 atttcgagtc ggcggtcctg gtcagcgcaa tcggccagct caacgaccca tcaccagctc
    11641 atttcaaagg cgaagaagat ttttccggcc ggatgttcca ctccgcgctg tggtcgaacg
    11701 atatcaagct cgatgccaaa cgcgtggctg tgatcggcac tggcgccacc gcgatgcagc
    11761 tcgtgccttc cattgccgat cgcgtggctt ccgtcacggt gtatcaaagg tcggcgcaat
    11821 ggtcgcgccc cgtcacgggc tattccgatc ccatcagcga aggcgcgcaa tggctgctcg
    11881 cgcacctgcc gttctacgtg caatggtatc gcttcaacat gttctggcgt tacggcgacg
    11941 ggctactgcc tttcctgcgc aaggatccga actggccgta tcccgatcgc gccgtcaaca
    12001 agggcaatga ccggcatcgc caggagctga cggattttat cctgaccgaa ctgaaagacc
    12061 ggccggacct gatcgaaaaa tgcgttccga cctatccgcc ctatggcaag cgcatcctgc
    12121 ttgacaacgg ctggtttcgt acgctcacaa aaccgcatgt cgaactggtc accgagccga
    12181 tcggccactt cgcccgcgat ggcatcgtca cctccgacgg caagttgcgg cccgcggatg
    12241 tcatcgtgat ctcgaccggg ttcaaggtca cgcagatggc ggcgcgtctc aatatcagcg
    12301 ggaacggcaa gaagctcgcc gatgcatggg ccgatgacaa tccgaccgcc tatctcggac
    12361 tcacggtgcc ggacttcccg aatttcttct gcatgctcgg ccccaacgcc ggcccagcgc
    12421 atggcggcag cgtgatcttc cagtcggaat gccagtcacg ttacatcacg gcgtgtcttg
    12481 tcaacatgat cgagcgaggc atagccgcca tcgacgttcg tcaggacatc catgaccggt
    12541 atgttcgcga ggtcgatgcc gagcatgaac aaatgatatg gacccatccg gggatgacga
    12601 cttactatcg aaacaagcag ggccgagtat tttccgcgat gccgtggcgg ttcgtggact
    12661 attggcgaat gacgcatgat ccggatttga gccagtatcg ccagaccaag gcctgaccac
    12721 cgccaacggg ggatccggta tgacaagacc acgcgtacga atctacaccg actacaaaag
    12781 cccctacgcc ttcgtcgcca acaagcggct cttcgagctg gaagaaaacc acggcgtcga
    12841 actcgaatgg ctgccctaca cccttcgcat cgccgaattc atgggcaagg tggaggagcg
    12901 cacgccgcat ttctggcgca aggtgcgtta cgcctatatg gacgcgcggc gttatgccaa
    12961 cacgcaaggg ctcaccatga aagggccgcg gcgaatttac gatgccttct attccagcgc
    13021 cggcatgctg tttgcacaac gccacgggct gttccgccct taccacgaca cggtgttcag
    13081 gcgcttctgg agtcacgacc tcgaaatcga cgaattgtcc ggcatttccg atgtgatcgc
    13141 ttctgtcggc ggctcagcga cggatttcga agcctacgtc aacggtccgg cgcgcgctga
    13201 gcatgaccgc atcatcgacg aggcggaagc cctcggcgtg ttcggggtgc ccaccatggt
    13261 attcaacggc gaactgttct ggggcggcga ccgcatcgac atgctgatcg aacgcatcaa
    13321 aaagcccgaa accatcgcgg ccgcgctggg aagccggcac cgagcatgaa acggccgttg
    13381 ccgccaagac tgatatcatc ggctactttg caacgagatg ttcccagcag ccggtacgct
    13441 ccaggacagg ccgcagcgcc gttcgatgct ccgcgtcgag ggcggcaaac ttgccgcgga
    13501 tcagatcgag gcatttgacc tgatacttga acggcgacag ggcgtaaggc aggccccaga
    13561 cactgatctc cagtcgctcg acacctttcg caaaggcttg cgcattggcg gcaagaaagg
    13621 gcagatagag ttcgccggcg atcccaagca gcgcctcagc catgccgctc agtgcctgct
    13681 cgcgcggata ccatttcccg tcgactcccg aggcgtcgtc caaccgtcta acccagtgat
    13741 cggtaaacgg cgctttctcc cgcatgatcc gcatcggggt gggatcggtc gccaattcgc
    13801 tcaattggcc gaaccatgca aaatccgcca gcgatggccg gcttccgaac agatattttg
    13861 tcattccaac gtgcggctcg aacgccgcca ggattcgacg atagctctct tccagcaaag
    13921 gcttgttttc ggccgtcgcg ccgagaatgg gcatgcggga aatctgcctc acgcggaaat
    13981 gctcgatctc ctcggcgctt ccggccacgg cgtccgacac cgaccattct tcagctgccc
    14041 atcgcgagac ataggcttga tcttcggggt cacaccaccg atagagaaaa agcggcttcg
    14101 cggcccattc gtcggcgaga tcctcgagga gatcgcagac gaacgctgtg gccttatctt
    14161 cgggaacgac cgatccgggt gccgggtttc gagatcgcac gccagtgtcg tggtttcgcc
    14221 gcgaaagctt ccgtcgggat attgcaggac aggaatcaaa ttcggacgca ggtgcccagc
    14281 ctgtttccgc agcgccctgg tcatgatgac ccagtcgaat gggatcttcc ggtagcgcaa
    14341 tagtgcccgc agcttgatcg cgtaaggcga tgccgtggat ccgatcagcc gatagcgtcc
    14401 ttcattcatg caagatatcc tgaccaccgg gcgcgatcgc accgatcgcg cttcacattc
    14461 gccatcgccg cgtgtttctg cctagcccag gcccaaaacc cgcgcaaccg gtcgccacct
    14521 ttcgctgaag ggccggaaaa ccgacgagct ctgcatttta cgaagacgga ccgtcttcca
    14581 tgtgagcaac gcggaccttg ttgtatcgcg tgaaatcatc cctaatgcgc ggaaggtcat
    14641 gcaacaacga gtgcagaaaa gagaccaaag ggagacgcgc gatgaagggt cccgaggacg
    14701 attccggctt gaccgacagg gtggccatcg tgagcggcgg tggtgctgcg ggcgacggca
    14761 ttggcaatgg ccgggctgcg gcaatcctgc tagcccgcgc gggcaccaag gtgctggtgg
    14821 ccgatctcga cctcaggctt gccgaacgca cggttgaaat gatccaggct gagggcggca
    14881 cggcggcggc actgtcgggc gacatcacca aggaatccga ctgcatgcgg ctggtcgaag
    14941 cagctgtcga ccgctggggc cggctcgact tcctcgacaa caatgtcggc atcggcagcc
    15001 gcggcagcgt cgtcgacgag aagcccgagg aatatcgacg ggtgatgcaa atcaacgtcg
    15061 agaccatgtt cctgctctcg aagtacgcga ttccggcaat gatcaagacc gccgggggcg
    15121 gctccatcgt caacatctcg tcgatatccg cgctccggcc gcgcggcctc accacctaca
    15181 cgacctcgaa agccgcagtc atcggactga cccaggcgat ggcggtcgat cacggcggcg
    15241 acaacatccg ggtcaattgc atctgccccg gtccgatgta cacgccgatg gtctatgcgc
    15301 gcggcatgag cgacgcagca cgcacccaac gcgccaacgc ctcgctgctg aaaaccgagg
    15361 gaaccggttg ggacgtcggc cacgccgtga aattcctgct cagcaactac gcccgctaca
    15421 tcaccgggca ggtgctggta gttgacggcg gcgtcacgct gcaaggcccg gaacgcgatt
    15481 cacgggatca ctgatcactt cataagggga gaaccaacac gatggcccgc ctgccctatc
    15541 ttgaggccga ccaggtcgcg cccgaatatc gcgacatgct caagcgcaac accaacctgc
    15601 acaagctctt ggtcaactcg cccgacatgg cgcgtgcctt caacggcatc ggtggctaca
    15661 tccgcttcaa gagcaaactc gacccgcgcc tgcgcgaact tgctatactt caagtgggct
    15721 ggctggagaa atcggaatac gaattcaccc atcatgtgag tatcggcaag gagtttggcg
    15781 tcaccgatga ggatataagg ggcctgatag ccgagaccga gggcaagccc tcccagcttg
    15841 agccgcttgc caaggcgatc gtgaaaggcg cacgcgaaat ggtgcgcgag cttgcgatgt
    15901 cggattcaac tttcgccgag atcaagcagg aactctctga cgagcatatg accgatctcg
    15961 tgctcaccat tgccttctat tgcgccgtcg tccgcgtgct cgccaccatg aagatcgaca
    16021 acgaacccac ctataaagag gtactgcagc agtacccgat gccgggagta aagtaacatg
    16081 cgcctgaaag atcgcgtcgc catcgtcgtt ggcgctggcc agagccccgg cgaaggcatc
    16141 ggcaacggcc gcgccaccgc cctcaccttc gcgcgcgaag gcgccaaggt actgtgcgtg
    16201 gatcacaata tcgagtcggc ccaggagacg gtcgacatga tcgatgccaa gggcggcacg
    16261 gcggtggcct acaaggccga cgtcaccaaa gaggccgaga tcaaggccat ggcagccgat
    16321 gcgcacaccc gctggggccg catcgacatc ctgcacaata atgtcggcgt cagcctcgcg
    16381 ggcggcgacg ccgagctgct cgacctcacc gaggaggcac tcgaccgctg cgttgcgatc
    16441 aacctcaaga gctgtatctt cgcagcgaaa cacgtgatcc cgatcatgcg ccggcagaac
    16501 agcggcgcca tcatcaacat ctcctcgatg gccgtcatca ccacctatcc ctatgtcgcc
    16561 tacaaggcga ccaaatcggc gatggtcgcg ttcaccgagc aactcgccta ccagaacgcc
    16621 cagtacggca tccgcgccaa cgccatcctg cccggcctga tgaatacacc gatggcggtc
    16681 gacacccgcg cccgcgaatt caagaaaagc cgcgccgaag tcgaagccga acgcgacgcc
    16741 aaagtgccgc tacgcaagaa gatgggcacc ggctgggacg tcgctaacgc cgcgctcttc
    16801 ctggcttccg atgaagccag cttcatcacc ggcgttacgc ttccagtgga cggcggcgca
    16861 agcgtgcggc gcgggtaaca gaacatggcc gcctcgcacg aaacgaaatc tccgcctggc
    16921 gcggcaccgg ctctcgagta cgacgccatc atcatcgggg cgggcatgtc gggcatgtac
    16981 cagctttacc gattgcgcga actcgggatg cgggtgcagg tgttcgaggc cggcaccggc
    17041 gtcggcggca cctggtactg gaaccgctac ccgggcgcgc gcttcgattc cgaaagctat
    17101 tcctacggct attccttttc gaaggaactg ctgcaggaat gggactggtc cgagcatttc
    17161 gctggccagc cggagacgct gcgctatctc aatcttgttg ccgacaagtt cgacctgcgc
    17221 cgcgacatcc agttccgcag ccgggttgcc gcggcgacgt accaggagga tacgcacagc
    17281 tggaacgtca cgctggcgga cggtagccac ttccgctcgc gcttcctgat caccgccatc
    17341 ggcccgctct c
//
LOCUS       DATNOI010000589        12601 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_951635,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000589 DATNOI010000000
VERSION     DATNOI010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12601
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951635"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..2802
                     /locus_tag="VN968_23860"
                     /pseudo
     CDS             1..2802
                     /locus_tag="VN968_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
     gene            3250..3759
                     /gene="lspA"
                     /locus_tag="VN968_23865"
     CDS             3250..3759
                     /gene="lspA"
                     /locus_tag="VN968_23865"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HXL32304.1"
                     /translation="MERSRNSRLGPGIIAAIAVLILDQASKVWLLFVFDIAHRGAVRV
                     TPFFDLVLAWNVGISFGWFQNDSQIAQISLMVIKAVAVIVLAIWMARSRTLIATLALG
                     LIIGGAVGNGIDRFAYGAVVDFALFHLQIGGNTYNWYVFNLADVAIVAGVAALLYDSF
                     LGVAAAKAP"
     gene            3858..4568
                     /locus_tag="VN968_23870"
     CDS             3858..4568
                     /locus_tag="VN968_23870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32305.1"
                     /translation="MRKASGRTVQKSSRALTHALRLAAIALGIGLVMTTGAVRAQDDD
                     EDDKTFEEKIIEGIMAGIGGTNMENRGIDYRERSPLVVPPKLDLPPPASASADVKAPN
                     WPKDPDEARRKAAIAARKKENKDPVEASRLLTPSELNKGKVAATARGSDDPIQPGSQN
                     NNPILSPSQLGYNGSFSGLFGGSKSETAPFKGEPTRESLTQPPSGYQTPSPNFAYGTG
                     PKESLNKEYNPAAGKYGE"
     gene            4749..6146
                     /locus_tag="VN968_23875"
     CDS             4749..6146
                     /locus_tag="VN968_23875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXL32306.1"
                     /translation="MMSPHRLAVSFFLFAALLSTFTLSAGGARAQTTVTSERPASFTL
                     GNGLQVVVIPDHRTPVVTQMIWYKVGSADETPGKSGLAHFLEHLMFKGTSKHPAGEFS
                     QTVLRIGGNENAFTSTDYTGYFQRVPREQLGKMMEFEADRMTGLILKDENVLPERDVV
                     LEEFNMRVANNPEARLVEQIMAALYLNHPYGRPVIGWHQEIEKLDREDALAFYRRFYA
                     PNNAIVIIAGDVEAKDVRPLAERTFGEVTAQPAIAAERVRPQEPVPAAPRTVTLADPR
                     VEQPSVKRYYLAPSATTAAAGESAALDVLAQLMGSGSNSYLYRALVVDRPLAVSADAG
                     YQGTALDPTQFMISVSPKPGVEFAQVEQVIDSVISDVGQNPVRAEDLERVKTQLIAEA
                     IYAQDNQATLARWYGGALTTGLSIDDLRSWPDRIRAVTAEQVRDVAQKWLDKKRSVTG
                     YLIKDATSKREEKRS"
     gene            6269..7591
                     /locus_tag="VN968_23880"
     CDS             6269..7591
                     /locus_tag="VN968_23880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXL32307.1"
                     /translation="MMAPLLSTPSHAAAKIQHLVSPGGIEAWFVQDATVPLIAMEYGF
                     GGGATQDPAGKPGVGNLVADLLDEGSGELDSKTFHEHLDRRAIELSFSSTRDYFRGSL
                     RMLKDNKDEAFDLLRMSLTSPHFESTDVERVRSQVLSGLRRDTSNPTSLATRKFLEVT
                     FGDHPYARQANGTLESVPKIDVADLKDYVRRVLAKDTLRIAVVGDVDPATLGKLLDQT
                     FGGLPAKASLTPVADVEAAKPPERAFIPLDVPQTVVTFGGPGIKRNDPAFMAGYVVNH
                     ILGGGGLSSRLYREVREKRGLAYSVYESLVWADHSAVFVGNTGTRADRAGETVDAIEK
                     EIRRMAEDGPTQQELDEAKSYLKGSQMLALDTSSKLASALLQYQLDQLPIDYIEKRNA
                     IVDAVTLDEAKKAAKRLWAQGLLTVIVGRAPQAAAQPAAATPPPPRAN"
     gene            7831..8250
                     /gene="arfB"
                     /locus_tag="VN968_23885"
     CDS             7831..8250
                     /gene="arfB"
                     /locus_tag="VN968_23885"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HXL32308.1"
                     /translation="MLRISRDLTIDENDIEISFVRASGPGGQNVNKLSTAAQLRFDTR
                     RVTLPADAEARLNRLAGQRMTKDGVIVIHAQRFRTQERNRADAVDRLLELLRAAMTRP
                     VPRRATKPTLGSKTRRLEGKKRRSDIKAKRGTARFDD"
     gene            8470..8754
                     /locus_tag="VN968_23890"
     CDS             8470..8754
                     /locus_tag="VN968_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXL32309.1"
                     /translation="MLFKQQCATCHTTNTSDPSRQGPTLFNIVGRDAGTAEGFRYSAG
                     FADAKFKWDEGKLDAWLADPQAMIPGAIMPYKQAKPELRVAIVNYLKELH"
     gene            8755..9150
                     /locus_tag="VN968_23895"
     CDS             8755..9150
                     /locus_tag="VN968_23895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL32310.1"
                     /translation="MAKPVHSMIRVLDEARSLDFYARAFGLEVADHLKFADFALIYLR
                     HPSSPFEVELTVNFDRKAAYEVGDGYGHLAVVVDDLDAEHARFSREKLSPGPLRDFRH
                     DGATLARFFFVSDPDGYKIEVIQKGGRFG"
     gene            9189..9737
                     /locus_tag="VN968_23900"
     CDS             9189..9737
                     /locus_tag="VN968_23900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HXL32311.1"
                     /translation="MREVDRRSKYSRRTFLQGAAAVAPVAAVATSAGLGIEDAWAADA
                     TTLAPATLKTLVKVARDIYPHDFLVDSYYITAIKPWDAKAAKDPATKALLEDGVRRLD
                     QDAQDRHKMVYAQVPWEADRVVLLQGIEQTEFFKKVRSDLVVSLYNQEELWPKFGYEG
                     SSAEHGGYIKRGFNDIDWLPKV"
     gene            9833..11401
                     /locus_tag="VN968_23905"
     CDS             9833..11401
                     /locus_tag="VN968_23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXL32312.1"
                     /translation="MAKFDLNDDGVVVIVGSGAGGGTLGNELAQKGVKVVILEAGPRV
                     ELQDFVNNEWESFTQLAWTDMRTTSGNWRVAKDFANLPAWIVKSVGGSTVHWAGASLR
                     FDEHEFRIKSAYGSVPGANLLDWPITLAEMEPWYAKAEDKMGVTRTNNIPGLPGNNNF
                     KVMEAGARKLGYKEVHTGRMAINSEPRDGRGSCLQIGFCFQGCKSGAKWSTLYAEIPK
                     GEATGNLEVRPGSMALKIEHDASGKVTGVVYADEAGKTQRQKARVVAVAGNSIESPRL
                     LLNSASNTFPDGLANSSGQVGRNYMRHMTGSVYAVFDKSVHMYRGTTMAGIIRDEARH
                     DPSRGFMGGYEMETLSLGLPFMAAFLNPGAWGRPFTAAMEGYPRMAGMWLVGEDMPQE
                     TNRVTLDPKAKDKFGLPVASVHYDDHPNDVAMRDHAYKQGSAVYEAIGATVTYPTTPY
                     PSTHNMGTNRMSAKAKDGVVNKFGQTHDIKNLFVSDGSQFTSGAACNPTLTIVALAVR
                     QADHIAGAMQRKEI"
     gene            complement(11474..11782)
                     /locus_tag="VN968_23910"
     CDS             complement(11474..11782)
                     /locus_tag="VN968_23910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HXL32313.1"
                     /translation="MCQLFAHQPQRDYESQTRSLRIGGHCTSIRLEMSFWDTLEEIAG
                     KEGLSLPKFLTKLHTEVLDHHGEVNNFASLLRCSCLIYRSKNAVVTPDFRAPVLDAAE
                     "
     gene            complement(11993..12205)
                     /locus_tag="VN968_23915"
     CDS             complement(11993..12205)
                     /locus_tag="VN968_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018484778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32314.1"
                     /translation="MFASKTLIEHVLRGAVGIGALCYAVAIAEVHPFASLASGALVLV
                     AFRGCPVCWTIGLFETARQHISKYRR"
     gene            complement(12236..>12601)
                     /locus_tag="VN968_23920"
     CDS             complement(12236..>12601)
                     /locus_tag="VN968_23920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXL32315.1"
                     /translation="SYDVLSKELARLGTGCAPPRGLFAVARRACHRLLRMSAGIPEAP
                     VDAMHARLAHLRPEDIRIDLSHAIQSLPGHYREVILLRDIEEMSIDEIAAILGLTRES
                     VKARIHRARMLIREYLIRD"
BASE COUNT         2589 a         3987 c         3861 g         2164 t
ORIGIN      
        1 gaggaagaga actaccgctc caagggcaag caaaagcctg attttcggga ctccaccgcc
       61 atggtggcgt tccggaagga atgccgcgcc tatgccgggc actggctcga cgtgcagcgc
      121 gaggaattca agcggctcgg catcatcggc gactgggatc atccctacgc caccatggat
      181 tatttcgccg aagcccagat cgcgcgcgag ctgatgaaat tcgccgccaa cggcacgctc
      241 tatcgcggct caaaacccgt gatgtggagc gtggtggaaa aaaccgcgct ggccgaagcc
      301 gaggtcgagt acgaggatta taccagcgat acggtgtggg tgaagttccc ggtggtgtct
      361 caaaggatcc aaacgccagc agaattccaa agcacactaa ctcaggatga actctcggat
      421 attggcgaga gtgtcttaga agaaatcgtc gaagtgttag atgcggaaga tgcccgcttc
      481 gcgctgtacg agagggcatt tattgtcatc tggacaacga cgccttggac ccttccgggt
      541 aatagagcga tctcgtattc actagacata gattacggac tgtatgaagt cactgcagcg
      601 cctgtaggga attgggcgaa agtgggcgaa cgttatgtcc tcgcggataa gttagcttca
      661 gaaatcttca aagctgcgaa agtcgagtcc taccgacgtc tattcgacgc taatgatgac
      721 ctacttaagc tcggcgcgcg ccaccctctt tctgagaagc taaacggtta tcagttctat
      781 gtccccctgc tcgaaggcga tcacgtcacc gacgacaccg gcaccggttt tgtccacacc
      841 gcgcccggcc atggccgcga ggacttcgac gtctggatgt cgaatgcgcg tcagctggag
      901 gcgcgcggca tcaacaccac gattccctac accgtcgacg agaacggcgc cttcaccgac
      961 caggcgccgg gttttaccgg aaaacgcgtc atcaacgaca agggcgaaaa gggcgacgcc
     1021 aacgacgccg tgatcaaggc gctggtcgac gccggcatgc tgctggcgcg cagccgcctc
     1081 aaacatcaat atccgcattc gtggcgctcg aagaagccgg tgatcttccg caacacgccg
     1141 caatggttca tcgcgatgga caaacctatt gtggacggcc cgggcaggcc cggggctatg
     1201 gacatcgcgg accctccttc acctctccca ttgggagagg tgaaggaggg cgacaccttg
     1261 cgcgcccggg cgctgcacgc gatctcggtc acccaatggg tgccgccgtc gggccagaac
     1321 cgcatcaacg gcatgatcga tagcaagccc gactgggtga tctcacggca gcgcgcctgg
     1381 ggcgtgccga tcgcggtgtt cgtgcgcgaa aacggcgacg gctccgccga aatcctgcag
     1441 gatgacgccg tcaacgtgcg catcgcaaag gccttcgagc aggaaggcgc cgacgcctgg
     1501 tacatggacg gcgctcgcga acgctttctc ggctcgctcg ccaatgagga gtggaagaag
     1561 atcgacgaca tctgcgacgt ctggttcgat tccggctcga cccatgcctt cgtgctggaa
     1621 gaccgcgtgc attttccgga gatcggcgac atccggcgca aggtcgatgg cggcgacgac
     1681 accgtgatgt atctggaagg ctccgaccag caccgcggct ggttccagtc gtcgctgctg
     1741 gagagctgcg gcacccgcgg ccgcgcgccg ttcgatatcg tgctcaccca cggcttcacg
     1801 ctggacgaga acggccgcaa gatgtcgaag tcgatcggca acaccgtcga gccgcaaaag
     1861 gtcatcgcgc agtccggcgc cgacatcttg aggctctggg tctgcgccac cgactacgcc
     1921 gacgaccagc gcatcggccc ggagatcctc aaaaacacca tcgagaccta ccgcaagctg
     1981 cgcaactcga tccgctggat gctcggcacg ctgcatcact tccatcgcag cgacatggtc
     2041 gcgcacgccg agatgcccga actggagcgg ctgatgctgc atcagctcgc aggccaggcc
     2101 gcgaccgtgc gcaaggctta cgccgaattc gattacaaga ccgtggtcgc ctcgctgtcg
     2161 gcgttcatga acaccgaact gtcggcgttc tatttcgata tccggaaaga tgcgctgtat
     2221 tgcgatccgc cgtcggcgct ggcgcggaaa gtagccctca ccaccatcga tattctctgc
     2281 gatgcgatcc tgaaatggct ggcgccggta ttgtcgttca cctccgaaga ggcgtggcgg
     2341 ctctacagac cggacgccga gccgtcggtg catctcacgc tgtttccgga aagttttgag
     2401 aatttccgcg acgaaaagct agcggcaaag tgggaagtca tccgcgccgt ccgccgcgtc
     2461 gtcaccggcg cgctggaagt cgagcgcgcc gccaagaaca tcggctcgtc gctggaagcc
     2521 tcgccgctgg tctatgtctc ggacaagaag attttcgaca cgctgttcga tatcgatctg
     2581 gccgaggtct gcatcacctc gaacgcgatg gtgaccaatg acgacgcgcc atcggatgcg
     2641 ttcaggctga acgaggtgcc cggcgttgcc gtagtcgtcg agaaggccgt cggcaccaaa
     2701 tgcgcgcggt cgtggaagat cctgccgacc gtcggcgacg accctgaata ccctgatgtc
     2761 tcgccgcgcg acgcgcaggc gctgcgcgaa tggaaagcgc tggagcaagg gcccgcggcg
     2821 cccgtcgctc cggttccagc gcagttgcgg gtggtcgagg ccatcgctac cgaacccgag
     2881 ccggaaattt ccgggcaaca ggacatcgag aagcaaccgg ctgaggtggc tgtacccagg
     2941 aagataaagc ctgccaagag cggaagcaca aagacaacgg gtgaaaccac gaagaagcct
     3001 gctgagaaga agtccgccaa aaagtccggc acgacggcaa gcaagaaggc ccccgccaaa
     3061 aagaaagccg ccaagacagc ggcggcaacg aagggcgcca aaaggaaacc tgcaaagcag
     3121 aaagcagcaa agaaggtaac caagaaggtt gccaagggga cgaagaagcc ggcgaagaag
     3181 gtctctgcag cgaaagcaaa aaagacgaaa aagaaggccc gaacaaagca ggccagataa
     3241 gtgtctggca tggaacgttc tcgcaactcc cgcctcggcc ccggcatcat cgccgcgatc
     3301 gccgtgctta tcctcgatca ggcctcgaaa gtctggctgc tgttcgtgtt cgacatcgcc
     3361 caccgcggcg ccgtgagggt cacaccgttc ttcgatctgg tgctggcctg gaatgtcggg
     3421 atcagcttcg gctggttcca gaacgacagc cagatcgccc agatcagcct gatggtcatc
     3481 aaggccgtgg cggtgatcgt gctggcgatc tggatggcgc ggtcgcgaac cctgatcgcg
     3541 acgctggcgc tcggcctgat cattggcggc gccgtcggca acggcataga ccgcttcgcc
     3601 tatggcgcgg tggtcgattt cgccctgttc cacctgcaga tcgggggaaa tacctacaat
     3661 tggtacgtgt ttaacctggc cgacgtggcg attgttgccg gggtagcggc cctcctgtat
     3721 gactcgttcc tgggggtagc cgccgcaaaa gcgccctgat cccggctgat acggaccgtc
     3781 tgggctggac gttgcttgca ggcggggagc tgtgcctcga atcggcagcc tgctgaaaca
     3841 tttgaacggg aaaagcgatg cgcaaagcca gcggccggac cgtgcagaaa tcgtcgagag
     3901 cgttgaccca cgcattgcgg ctcgccgcca tcgcactcgg catcggcctt gtcatgacga
     3961 ccggggcggt ccgcgcccag gacgacgacg aggacgacaa gaccttcgag gaaaagatca
     4021 tcgagggcat catggccggt atcggcggta ccaacatgga gaacaggggg atcgactatc
     4081 gcgagcgctc cccgctggtg gtgcccccca agcttgatct gccgccgccc gcctccgcat
     4141 cggccgatgt gaaggccccg aactggccga aagatccgga cgaagcacgc cgcaaggctg
     4201 cgatcgcggc ccggaagaaa gagaacaagg atcccgtcga agcgagccgg ctcctgaccc
     4261 cgagcgaact caataagggc aaggtagccg ctacggcccg cggcagcgac gaccctattc
     4321 agccgggcag tcagaacaac aatccgatcc tcagcccgtc gcagcttggc tataatggca
     4381 gcttcagcgg tctcttcggc ggcagcaaga gcgagacggc tccgttcaag ggtgagccga
     4441 cccgtgagtc gctgacgcag ccgcccagcg gataccagac gccgtcgccg aattttgcct
     4501 acggcaccgg gccaaaggaa tcgctgaaca aggaatataa tccggccgcc ggcaagtatg
     4561 gcgaatagcc gcgcccggct ctcggcttcg tgacgctccc gggcagccgc gccgtgtagg
     4621 gtggccgcga ctttatcccg aacatttcgg ttcgggggcc gtcgctctca ttcgaaatat
     4681 tgcgcgtatc cttcgtcctt cgcaaaatcc ggtgcccaat tttgcgcaaa acacgcgcta
     4741 gacaggtcat gatgtcccca caccgcctcg ccgtctcctt cttcctcttc gccgcgctgc
     4801 tatcgacgtt caccctctcc gcaggcggcg ctcgcgccca gaccacggtg acttccgagc
     4861 gtcccgccag ctttaccctc ggcaacggcc tgcaggtcgt ggtgatcccg gatcatcgca
     4921 cgccggtcgt cacccagatg atctggtaca aggtcggctc cgccgacgag acgcccggca
     4981 aatcgggcct cgcgcatttt ctcgaacatt tgatgttcaa gggcaccagc aagcacccgg
     5041 ctggtgaatt ttcccagacc gtgcttcgga tcggcggcaa cgagaacgcg ttcacctcga
     5101 ccgactacac cggctatttc cagcgcgtgc cgcgcgagca gctcggaaag atgatggagt
     5161 tcgaggccga ccgcatgacc ggcctgatcc tgaaggatga aaacgtgctg ccggagcgcg
     5221 acgtcgtgct cgaagaattc aacatgcggg tcgccaacaa cccggaggcg cggctggtcg
     5281 agcagatcat ggcggcgctg tatctcaatc atccctatgg ccgcccggtg atcggctggc
     5341 accaggaaat cgaaaagctc gaccgcgaag atgcgctggc gttctacagg cgcttttacg
     5401 cgccgaacaa cgcgatcgtg atcattgccg gcgatgtcga agccaaagat gttcgcccgc
     5461 tggcggagcg gacgttcggc gaagtcaccg cccagcccgc gatagcggcg gagcgcgtgc
     5521 ggccgcagga accggtcccg gcggcgcccc gcaccgtgac gctggccgac cccagggtcg
     5581 aacaacccag cgtgaagcgc tattacctgg ctccgtcggc caccaccgcc gccgcgggag
     5641 aaagtgcggc gctcgacgtg ttggcgcagt tgatgggcag cggcagcaac tcctatcttt
     5701 atcgcgcgct ggtggtcgat cgcccgctcg cggtcagcgc cgacgccgga tatcagggca
     5761 ccgcgctcga tccgacgcag ttcatgatct cggtctcgcc gaagcccggc gttgaattcg
     5821 cgcaggtcga gcaggtgatc gatagcgtaa tatcggatgt cggacaaaac ccggtacgcg
     5881 ccgaggatct cgagcgggtc aagacccagc tgatcgccga ggcgatctac gcccaggaca
     5941 atcaggcgac gctggcgcgc tggtatggcg gggcgctcac caccggcttg agcattgacg
     6001 acttgagaag ctggccggat cgcattcgcg ccgttaccgc cgaacaggtc cgcgacgtcg
     6061 cgcagaaatg gctcgacaag aaacgttcgg tgaccggcta tctgatcaag gatgctacgt
     6121 caaaacgcga ggagaagcgt tcgtgatcga tgtcaggact tgtcccaaga cttattctcc
     6181 caagacttat tctccgaaga cttattctcc gaagacttct ccccggcccc gtgcgcgccg
     6241 cacggcgttg attgttgcga cctgcctcat gatggcgccg ctgctatcaa cgccatcgca
     6301 cgccgcggcc aagattcagc atctggtttc acctggcggt atcgaggcct ggttcgtgca
     6361 ggatgccacc gtgccgctga tcgccatgga gtatggcttc ggcggcggcg cgacccagga
     6421 tccggccggc aagcccggcg tcggcaatct ggttgccgat ctgctcgatg aaggctcggg
     6481 cgagctcgat tccaagacgt tccacgaaca cctcgaccgc cgcgccatcg agttgagctt
     6541 ctcttcgacg cgtgattatt tccgcggctc gctgcgcatg ctgaaggata acaaggacga
     6601 agctttcgac ctgctgcgga tgtcgctgac atcgccgcat ttcgagagca ccgatgtcga
     6661 acgtgttcgc tcgcaggtgc tatcgggcct gaggcgcgat acctccaatc cgacctcgct
     6721 ggccacccgc aagttcctgg aagtcacctt tggcgaccac ccctatgcca ggcaggccaa
     6781 cggcaccctg gaaagcgttc caaaaatcga cgtcgccgat ctgaaggatt acgttcgccg
     6841 ggtgctggcc aaggatacgc tgaggatcgc cgtggtcggc gatgtcgatc ccgcgacgct
     6901 cggcaaactg ctcgatcaga cgtttggcgg actgccggcc aaggccagcc tgacgccggt
     6961 cgccgacgtc gaggccgcca aaccgccgga gcgtgcgttc attccgctcg acgtgccgca
     7021 gaccgtggtg acgttcggcg gccccggcat caagcgcaat gatccagctt tcatggccgg
     7081 ctatgtcgtg aaccacattc tcggcggcgg agggctgtcg tcgcggctct accgcgaagt
     7141 ccgcgagaag cgcggtcttg cctattcggt ctatgaatcg ctggtgtggg cggatcactc
     7201 cgcggtgttc gtcggtaata ccggcacccg cgccgaccgc gccggcgaga ccgttgacgc
     7261 catcgagaag gaaatccgcc gcatggccga ggatggcccg acccagcagg agctggatga
     7321 ggccaagtcc tatctgaagg gttcgcaaat gctggcgctc gacacctcgt caaagctggc
     7381 ctcggcgctg ctgcaatacc agctcgatca acttcccatc gactatatcg agaagcgcaa
     7441 cgccatcgtc gatgccgtga ccctcgacga agccaaaaag gccgccaagc ggctgtgggc
     7501 gcaaggcctc cttaccgtga tcgtcggccg cgccccgcaa gccgccgccc agccggcggc
     7561 ggcgaccccg ccgccaccca gggcgaactg aatccctttc gcgttttggc gaagaaactg
     7621 ctgcgcggca ccgcctgctc tctcccccct tgcgggggag agttggagag gggggtagcc
     7681 gcgcgtccgg cgcttgttgc tcacccccct tccctaaccg caagggggca gggaacgact
     7741 gacgcaagcg gcaacgcaaa cttctcacaa aaccgctatc ctgcgcctgt ccgattcccg
     7801 tttgggatca tcccggcctc cggtgacgcc atgctgcgga tttcccgcga cctcacgatc
     7861 gacgagaacg acatcgagat cagtttcgtc cgtgcctccg gtccgggcgg acagaacgtc
     7921 aacaagctct cgaccgcagc ccaactgcgg ttcgatacca ggcgggtcac gctgccggcg
     7981 gacgccgagg cgcgcctcaa ccggctcgcc ggccagcgca tgaccaagga cggcgtgatc
     8041 gtgatccatg cgcagcgttt ccgcacccag gagcgcaacc gcgccgacgc cgtcgaccgc
     8101 ctgctcgagc ttttgcgcgc cgcgatgacg cgccccgtcc cgcggcgcgc gaccaagccg
     8161 acgctgggct cgaaaacgcg ccgtctcgaa ggcaaaaagc gccgcagcga catcaaggca
     8221 aagcgcggca ccgcgcgatt cgacgattag cgggctgtgg ctcgccgcgc tttgttccgc
     8281 cctgcaacag cccactacta ccaccgcccg gttgccgcgg cctaaggtct gctcgcgcca
     8341 acggtgatgg acatgacaaa acgacgcgcg gcactgcgga tcgggctggc attttgcgct
     8401 gcggcgctgg ccgtcgacgg cgctgcggcg caattgaccc cgcctccgaa aaagccgccc
     8461 gatggcgcga tgctgttcaa gcagcaatgc gccacatgcc acaccaccaa cacctccgat
     8521 ccgtcgcggc aggggccgac cctgttcaac atcgtcggac gcgacgccgg aaccgcggag
     8581 ggatttcgct attcggctgg ttttgccgat gcaaaattca aatgggacga aggaaagctc
     8641 gacgcctggc tggccgatcc gcaagcaatg atccccggcg cgatcatgcc ctacaagcag
     8701 gccaagcccg aacttcgcgt cgcgatcgtg aactatctga aggagttgca ctgaatggcg
     8761 aaaccagttc actcgatgat tcgcgtgctc gatgaagcaa ggtcgcttga tttctacgcg
     8821 cgcgccttcg ggctcgaggt cgccgaccat ctcaaattcg cggactttgc gctaatctat
     8881 ctgcgtcatc cgtcctcgcc cttcgaggtc gaactgacgg tgaatttcga tcgcaaggcg
     8941 gcttacgaag tgggcgacgg ctacggccac ctcgccgtcg tggtcgacga tctcgatgcc
     9001 gagcatgccc gcttcagccg cgaaaaactg tcacccgggc cgctgcgcga tttcaggcat
     9061 gacggcgcga cgctggcgcg gtttttcttc gtctccgacc cggacggcta caagatcgaa
     9121 gtgatccaga aaggcggacg cttcgggtaa tcatgtcaat caacagaaac gttcaatgga
     9181 ggatacccat gagagaagtc gatcgtcgaa gcaaatacag tcgccgaacc ttcctgcagg
     9241 gcgccgcagc cgtagcgccg gtcgcggccg tcgccaccag cgccggcctc ggcatcgagg
     9301 atgcctgggc cgcggacgcg accacgctgg cgccggcgac gctgaaaacg ctggtcaagg
     9361 ttgcgcgcga tatctatccg cacgactttt tggtcgacag ctactacatc accgcgatca
     9421 agccgtggga cgcaaaggcg gcgaaagatc cggccaccaa ggcgctgctt gaagacggcg
     9481 tgcgccgtct cgatcaggac gcgcaggacc gccacaagat ggtctacgcg caggtgccgt
     9541 gggaagccga ccgcgtcgtc ctgctgcagg gcatcgagca gaccgagttt ttcaagaagg
     9601 ttcgctccga cctggtcgtc tcgctctaca accaggaaga gctgtggccg aagttcggct
     9661 acgagggctc ctccgccgag catggcggct acatcaagcg cggtttcaac gacatcgact
     9721 ggctgccgaa ggtctagagc cctttccgtt tcgattgaat cgaaacgggg ctccagattc
     9781 ttgacgcgcc ggaagacaac ttcgcgcaga acatcatttg ggaggacaca caatggcaaa
     9841 attcgatctg aatgacgatg gtgtggtggt tatcgttggc tccggcgccg gcggcggaac
     9901 gcttggcaat gaactggcgc agaagggcgt caaggtcgtg atcctggagg ccgggccgcg
     9961 cgtcgagctg caggacttcg tcaacaacga gtgggaaagt ttcacgcagt tggcgtggac
    10021 ggatatgcgg acgacatccg gcaactggcg ggtggcaaag gatttcgcca atctgccggc
    10081 atggatcgtg aaatcggttg gcggatcgac cgtgcattgg gccggcgcgt cgctgcggtt
    10141 cgacgagcat gaattcagaa tcaagagcgc ctatggcagc gtgccgggcg ccaatctcct
    10201 ggactggccg atcacgctgg ctgaaatgga gccgtggtac gccaaggccg aagacaagat
    10261 gggcgtcacc cgcaccaaca acattccggg attgccgggc aacaacaact tcaaggtgat
    10321 ggaagcgggc gccaggaagc tcggctacaa ggaagtccac accggacgga tggcgatcaa
    10381 cagcgaaccg cgcgatggcc gcggctcctg cctgcagatc ggcttctgct tccagggctg
    10441 caagtccggc gcgaaatggt cgacgctcta tgccgaaatc ccgaagggcg aagccaccgg
    10501 caatctcgaa gttcgtcccg gcagcatggc gctcaagatc gagcacgacg catccggcaa
    10561 ggtgaccggc gtggtctatg ccgacgaagc cggcaagacg cagcgccaga aggcccgggt
    10621 cgttgccgtc gccggcaact cgatcgagag cccgcggctg ttgctcaaca gtgcgtccaa
    10681 tacattcccc gacggccttg ccaacagctc gggccaggtc ggccgcaact acatgcgtca
    10741 catgaccggc agcgtctatg cggtgttcga caagtcggtc cacatgtatc gcggcaccac
    10801 catggccggc atcatccgcg acgaggcgcg gcacgatccg tcgcgcggat tcatgggcgg
    10861 ctatgaaatg gaaacgttgt cgctaggcct gcccttcatg gccgcgttcc tcaatccggg
    10921 tgcgtggggg cgcccgttca cggcggctat ggaaggctat ccgcgcatgg ccggaatgtg
    10981 gctggtcggc gaggacatgc cgcaggaaac caaccgcgtc acgctcgatc cgaaagccaa
    11041 ggacaagttc ggcctgccgg tggcgagcgt gcattacgac gaccatccca acgatgtcgc
    11101 gatgcgcgac cacgcctaca agcaaggctc cgccgtctat gaggcgatcg gcgcgacggt
    11161 gacctatccg accacaccct atccctccac gcacaacatg ggcaccaacc gcatgagcgc
    11221 gaaagcaaaa gatggcgtgg tcaacaagtt cggccagacc cacgacatca agaatctgtt
    11281 tgtgtcggat gggagccagt tcaccagcgg cgccgcctgc aacccgaccc tgaccatcgt
    11341 cgcacttgcg gtccgccagg cggaccacat cgccggcgcg atgcaacgaa aggaaattta
    11401 gtccgtctct gatatgaaaa ggctccgtcc gcgacgcggg cggagccctt atcatgccac
    11461 ccatcgagag cgactattcc gcggcgtcga ggacaggcgc gcggaagtcg ggcgtcacca
    11521 ccgcattttt cgagcgatag atcaggcagg aacaacgcag cagcgaggcg aaattattga
    11581 cctcaccgtg atgatcgagc acttcggtgt gaagcttggt caggaatttc ggaaggctga
    11641 ggccctcctt gcccgcgatc tcctccagcg tatcccagaa cgacatttcc agccggatcg
    11701 acgtgcaatg ccccccgatc cggagcgatc gggtctgcga ttcatagtcg cgctgcggct
    11761 ggtgtgcgaa cagctggcac atgggtatcc tcccgatctc ttttaaactt ttctaaacga
    11821 tatacccggc cgccggaggc gacaatgttc ccgcgtcggg cggggtcggt ttttcggcct
    11881 gccacaatcc gaaccggcgc catatttcct gtcgtgcgac ccaattccgc ggcgccgacc
    11941 gcgtccgctc ttctggggca ggattgccaa ggtaattccg aggcctggaa actcagcggc
    12001 ggtatttcga gatatgctgc cttgcggtct cgaacagccc gatcgtccag cacaccggac
    12061 agccgcgaaa cgccaccagc accagcgccc ccgaggccag cgaagcgaag ggatggacct
    12121 ccgcgatcgc cacagcatag cacagcgcgc caattccgac cgcgccacgc aggacatgtt
    12181 cgatcagcgt cttgcttgcg aacatgatct tgattccagt ctgccttgat cggtgtcagt
    12241 cgcggatcaa atattctcgg atcagcatcc gcgcgcggtg aatgcgggcc ttgacgctct
    12301 cacgggtcag gccgaggatg gcggcgatct cgtcgatcga catctcctcg atgtcgcgca
    12361 gcaggatcac ctcgcgataa tgcccgggca gcgactggat cgcatggctg aggtcgatgc
    12421 gaatgtcctc gggccgcaga tgcgccaatc gcgcatgcat cgcgtcaacc ggagcctcgg
    12481 gaatcccggc cgacatccgc aacaggcgat gacaggcgcg ccttgcgacc gcgaaaagcc
    12541 cccgaggagg cgcgcagccc gttcccaacc tggcaagttc tttcgataaa acatcgtagc
    12601 t
//
LOCUS       DATNOI010000590         3331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2598895,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000590 DATNOI010000000
VERSION     DATNOI010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2598895"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VN968_23925"
     CDS             <1..992
                     /locus_tag="VN968_23925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXL32316.1"
                     /translation="KADLFRSEKIPSTFNWDFSPKGQHIELGIAEMNLFILMSALGLS
                     HAINGERLLPIATLYDPFIERGLDALNYACYQDARFMVAATPSGITLAPEGGAHQSIA
                     TPLIGMAQDGLAYFEPAFVDELAVIMAFGFQYMQREGAGEGGSVYLRLSTRTIDQPQR
                     MMTPDLQKGITDGAYWLRRPGENCEAVIAYTGAVAPEAIEAIGLIGESRRDVGLLAIT
                     SADRLHSGWTAARKLRRDRRGVPHLSHIEKLLAPLPRDCGIVTVIDGHPATLGWLGSV
                     RGHKVEALGVEHFGQTGTIDDLYRHHGMDANAIIDAAEAITAGAPVRHRKMAV"
     gene            1483..2913
                     /locus_tag="VN968_23930"
     CDS             1483..2913
                     /locus_tag="VN968_23930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXL32317.1"
                     /translation="MVNRMQFYIDGAWVDPVVKKSTPVVNPATEETMYEVALGSKADV
                     DKAVAAARRAFETFSQTSREERVALLGKIIEVYKTRMKDIGAAVSDEMGAPLPMAEKL
                     QAGAGLGHLAATMEVLKNYHFEEQLPSAVVVREPVGVVGMITPWNWPLNQITCKVAPA
                     LAAGCTMILKPSESTPTSALIFAEILHEAGVPKGVFNLINGLGPEVGAAMSEHPDIDM
                     ISFTGSTRAGIDVAKRAAPTVKRVSQELGGKSPNVILEGADLAKAVSGGVMHMFNNSG
                     QSCNAPSRMIVPLSKMKEVAAIAKSVAEKTKAGDPRAEGTTIGPVVNRGQWDKIQTLI
                     HKGIEEGATLVAGGPGLPEGVNKGFYVRPTIFADITNDMTIAREEIFGPVLTILGAKD
                     EEDAVRIANDTPYGLAGYVSADTVETARRVGRRIRAGNVNLQGVPNDRTAPFGGYKQS
                     GNGREWGKYGLEEYLEVKAVAGYNAA"
     gene            complement(3088..>3331)
                     /locus_tag="VN968_23935"
     CDS             complement(3088..>3331)
                     /locus_tag="VN968_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXL32318.1"
                     /translation="TPDGMRCDIDGNLWCGWGMGDPELDGVVVYAPDGVLIGRIALPE
                     RCANLCFGGLKRNRLFLAASQSIYALYVNTQGALGG"
BASE COUNT          628 a         1084 c         1049 g          570 t
ORIGIN      
        1 aaaaggccga cctgttccgc agcgagaaga taccttcgac cttcaactgg gacttttcgc
       61 ccaaaggcca gcacatcgag ctcggcatcg ccgagatgaa cctgttcatc ctgatgtcgg
      121 cgctcgggct gtcgcacgcc atcaacggcg aacggctgct gccgatcgcg accttgtacg
      181 atcccttcat cgagcgcggc ctcgatgcct tgaactacgc ctgctatcag gacgcccgct
      241 tcatggtggc ggcgacccca tccggcatta cactggcgcc cgaaggcggc gcgcaccagt
      301 cgatcgcgac gccgctgatt gggatggcgc aggacggcct ggcatatttc gagccggcct
      361 ttgtcgatga gcttgccgtg attatggctt tcggattcca gtacatgcag cgcgagggtg
      421 ccggcgaggg cggttcggtt tacttgcggc tttcgacccg caccatcgac cagccgcagc
      481 ggatgatgac gccggacttg caaaagggca tcaccgacgg cgcctattgg ctgcgcaggc
      541 ccggcgaaaa ctgcgaagcc gtgatcgcct ataccggcgc ggttgcgccc gaggccatcg
      601 aggcgatcgg cctgatcggc gaaagccgcc gcgatgtcgg cctattggcg atcacctccg
      661 ctgaccgcct gcattcgggg tggaccgcgg cgcgaaaact gcgccgcgac cgccgcggcg
      721 tgccgcatct cagccatatc gaaaagcttc tggcgccgct gccgcgcgac tgcggcatcg
      781 tcaccgtgat cgacggtcac cccgccaccc tcggctggct cggcagtgtg cggggccaca
      841 aggtggaagc gctcggcgtc gagcatttcg ggcagaccgg gacgatcgac gatctctatc
      901 gccaccacgg catggacgcc aacgccatca tcgacgcggc cgaagccatc accgccggcg
      961 ctccggtgcg tcaccgcaag atggcggtgt agttgcttac cctcccctgg aggagggtcg
     1021 acgcgaatta aatgagcggc ggggtggggt gacggtcttt cgtttctaac ggtgcccgat
     1081 tcgagggatc accccacccc gtctcacatt tcactgcgct caatgtgagt cgaccctccc
     1141 cctccagggc agggctatcg catatggaaa atccaccaga tgcggcgggc gcttccggcc
     1201 gaggccagtg gtggacacac aacaaccgtt cccaattcga ttccgctttg ttcacgatgc
     1261 gattccttgg gcctcgccag gccaggtaag ccggtgcgaa gccaagcgat ggaaaagact
     1321 ccaagaatcc atatgcgata gccctgccct ccaggggagg gtaagtaacc gctgcagtgc
     1381 ggcgtcgcgc atatcgcggg ttcattttcc tgatcattcg tgcaaggatg cctgccgaac
     1441 gctccgttcc ttccatttgc ccctgacatc aagaggtttc cgatggtcaa ccgcatgcaa
     1501 ttctatatcg atggcgcctg ggtcgatccc gtcgtcaaga aatccacgcc tgtcgtcaac
     1561 ccggcgaccg aagagacgat gtatgaggtt gcgctcggct ccaaggccga cgtcgacaag
     1621 gccgtggccg ccgcgcgccg cgccttcgag acgttttcgc agaccagccg cgaggagcgc
     1681 gtggcgctgc tcggcaagat catcgaggtc tacaagaccc ggatgaagga tatcggcgcc
     1741 gccgtctccg acgagatggg agcgccgctg ccgatggcgg agaagctgca ggccggcgcc
     1801 ggcctcggcc atctcgccgc cacgatggag gttctgaaaa actatcactt cgaggagcaa
     1861 ctcccctccg cggtggtggt gcgcgagccc gtcggcgtcg tcggcatgat caccccgtgg
     1921 aactggccgc tgaaccagat cacctgcaag gtcgcgcccg cgctcgccgc cggctgcact
     1981 atgatcctga agccgtcgga atctacgcca acatcggcgt tgatcttcgc ggaaatcctg
     2041 cacgaggccg gcgtgcccaa gggcgtattc aacctgatca acggcctcgg ccccgaagtc
     2101 ggcgccgcca tgagcgagca ccctgatatc gacatgatct cgttcaccgg ctcgacccgc
     2161 gccggcatcg atgtcgccaa gcgcgccgcg ccgacggtga agcgcgtcag ccaggaactc
     2221 ggcggcaagt cgccgaacgt catcctcgaa ggcgccgacc tcgccaaggc cgtcagcggt
     2281 ggggtgatgc acatgttcaa caattccggg cagtcgtgca acgcgccgtc gcggatgatc
     2341 gtgccgttgt cgaagatgaa ggaagtcgcc gccatcgcca agagcgtggc ggagaagacc
     2401 aaggccggcg atccccgcgc cgagggcacc accatcggtc cggtggtcaa ccgcggacaa
     2461 tgggacaaga tccagacgct gatccacaag ggcatcgagg aaggcgccac gctggtggcc
     2521 ggcggtccgg gcctgccgga aggcgtcaac aagggttttt atgtgcgccc gaccatcttc
     2581 gccgacatca ccaacgacat gacgattgcg cgcgaggaga tttttggccc cgtgctcacc
     2641 atcctcggcg ccaaggacga ggaagatgcg gtgcggatcg ccaacgacac gccgtatggc
     2701 cttgcggggt acgtctccgc cgacaccgtt gaaaccgccc gcagggtggg ccgcaggatc
     2761 cgcgccggca acgtcaatct gcagggcgtg ccgaacgacc gcaccgcgcc gttcggcggc
     2821 tacaagcagt cgggcaacgg ccgcgagtgg ggcaaatacg gcctggaaga atatctcgag
     2881 gtcaaggcag tcgccggcta caacgccgcg taagttttac gatgattgca gcgccggggc
     2941 gggaattttc ccgctccggc gtttttatct ttgcgaagca gtgccgcgag caacttcgtc
     3001 attgcgagga gcgaagcgac gaagcaatcc attcttccct tgcggctcga tggattgctt
     3061 cgcttcgctc gcaatgacgg ggttgggcta tccccccagc gcgccttgcg tgttcacata
     3121 gagcgcgtag atcgactggc tcgcggccag gaacagccgg ttgcgcttca agccgccgaa
     3181 gcagaggttg gcgcagcgct cgggcagcgc gatgcggccg atcaagacgc cgtcgggcgc
     3241 gtagacgacg acgccatcga gctcgggatc gcccatgccc cagccgcacc acaaattgcc
     3301 gtcgatatcg cagcgcatgc cgtcgggcgt g
//
LOCUS       DATNOI010000591         8165 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1839835,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000591 DATNOI010000000
VERSION     DATNOI010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1839835"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VN968_23940"
     CDS             <1..882
                     /locus_tag="VN968_23940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32319.1"
                     /translation="RCRRLPRVAIRHTVGLDDQVEGGKGGVADIRENSGARYFKLKLN
                     GNPDADTARLIRIGRELATLSYDCSVTLDANEQYADLTALGALVDRLGADSALKPIAA
                     KLLYIEQPMPRDIFRQSPLGTLAAFDIIIDEADDCYDAFPVARALGYRGISSKSCKGT
                     YKSIVNTTRAAKWSAGGEKFFITGEDLTCQAGLAVQQDLALGALIGVTHAERNGHHYV
                     DGFGDTPAAEAQAFLAAHPDLYVSDGNNIRLSIHDGDLVTGSLAAPGFATSVHPEWTA
                     LSPLQQLKAKSLQEQSI"
     gene            879..2030
                     /locus_tag="VN968_23945"
     CDS             879..2030
                     /locus_tag="VN968_23945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXL32320.1"
                     /translation="MTTQRLGVIMNGVTGRMGLNQHLIRSIIAIRDSGGVPLANGDRM
                     MPDPILVGRDSDKVEGLARRFNVERWTTDLDKALLEPSDGIFFDAATTQARPSLLTKA
                     INAGKHVYCEKPIATNLEEAVAVLRLANAKGVKHGTVQDKLFLPGLKKLAFLRDSGFF
                     GRMLSVRGEFGYWVFEGDWQEAQRPSWNYRSEDGGGIILDMVCHWRYVLDNLFGEVES
                     VSCLGTTDIPERWDEKGEKYKATADDSAYATFRLKGGVIAHINMSWVTRVYRDDLVTF
                     QVDGTHGSAVAGLSDCVIQARQATPRPVWNPDEKRTHDFYADWQTLPENAVYDNGFKE
                     QWEMFIRHVCEDAPYRYTLLEGAKGVQLAECALQSWRERRWIDVAPIKV"
     gene            2043..3236
                     /locus_tag="VN968_23950"
     CDS             2043..3236
                     /locus_tag="VN968_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXL32321.1"
                     /translation="MNKPVLPVSSLSLKLPNADRSIETYRLAASRTFPSKLEDTLNRV
                     AFSAAHVVADPLADVDPWLTSAVDWDRTIAFREHVWDLGLGVAEAMDTAQRGMGLDWP
                     TSLELIQRSVKAAKARGNALVFSGAGTDHLEPRDAKTIDDVISAYEEQIAAVEKAGGR
                     IILMASRALAKIGRNPDDYAKVYNRVLSQVAEPVIIHWLGDMFDPALSDYWGTSSLDQ
                     AMDVAVGVINANASKVDGVKVSLLDKQREIDMRRRLDNDVKMYTGDDFNYAELIAGDD
                     QGFSHALLGIFDAIAPAASYALSRLAAGDEAGFHDVLGPTVPLSRHIFKAPTRFYKTG
                     IVFMAYLNGHQDHFTMVGGQESTRSILHLAELFRLADQAGLLANPELATRRMKAVLAT
                     RGVEA"
     gene            3236..4099
                     /locus_tag="VN968_23955"
     CDS             3236..4099
                     /locus_tag="VN968_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HXL32322.1"
                     /translation="MRDFSKDHRWLSLNTATVRKQGNLAEIIDACARHGIRAIDPWRD
                     QVATIGLDRAVRAVRDAGLELSGYCRGGMFTSDAAHRIEARDDNRRTVDEAKALGAPC
                     VVLVVGGLPQYSWPGSVASKDIAAARAQVDDAIAEMMVYARDANMPLAIEPLHPAYAA
                     DRACVNTTKQALDICDRLDPQRSGALGVALDVYHIWWDPELLPQIERAGKDRLLAFHV
                     CDWLVPTMDILNDRGMMGDGVIDIKSVRRAVEAQGFAGYSEIEIFSYDWWEEPMDEVL
                     QTCIARHRSVV"
     gene            4252..5139
                     /gene="yghU"
                     /locus_tag="VN968_23960"
     CDS             4252..5139
                     /gene="yghU"
                     /locus_tag="VN968_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HXL32323.1"
                     /translation="MTDPSEYTPPKVWSWSKENGGRFANINRPIAGPTHDKELPVGHH
                     PLQLYSLATPNGVKVTVMLEELLALGHRGAEYDAWLIRIDGNQFGSGFVAINPNSKIP
                     ALMDHSGPTPIRVFESGSILLHLAEKFGAFLPTDVAKRAECLSWLFWQMGSAPYLGGG
                     FGHFYAYAPTKIEYAIDRFAMEVKRQLDVLDRRLADNAYVAGDDYSIADIAIWPWYGG
                     LAKGVLYGAGEFLGVQEYKNLQRWADAIAARPAVRRGRMVNRTWGEPSSQLHERHDAS
                     DFETRTQDKVEDGQPATGA"
     gene            5300..7255
                     /locus_tag="VN968_23965"
     CDS             5300..7255
                     /locus_tag="VN968_23965"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXL32324.1"
                     /translation="MRRFLRALAIGLTLVALPGVGFAASDEETVRLLQRDADQGNANA
                     QVRLGYFYENGRGGLAKDDRKAARLYKLAADQGNAAARNNLGVLYRDGGGGLTKDDRE
                     ALRLFKLAADQGNAYAQQNLGGFYRDGRGGLGKDDREAVRLFKLAADQALADARASLG
                     YFYETGRGGLAKDTREAARLYRLAVDQGNAAAQNNLGVFYRDGGGGLTKDDREALRLF
                     KLAADQGNSYGQHNLGGLYRDGLGGLAKDEREAARLFKLAADHGNAGAGVSLGYFYEK
                     GLGDLAKDDREAVRLYRLAADQGNAAGQNNLGVLYRDGRGGLTKDEREAARLFKLASD
                     QNNAYGQQNLGIFYRDGRGGLTMDNREAARLFKLATDQGNANARTALNQITQPSQEVT
                     AVANARSNQQVVEPSSAPAANLPSVPERRVALVIGNAAYQAVSGLANTTNDARAIADV
                     LKADGFTSVRIALDMTRASMIASLNDFQREADTSDWAVVYYAGHGLEVSGTNYLVPID
                     AKLGDDRDIQDEAVSVDRILRAVENAKKLRLVMLDACRDNPFLKKMHRSIATRSISRG
                     LAAIEPLGATLIVFAAKDGETAEDGAGNHSPFAASLIERLQQPTIEINKLFRLVTSDV
                     LKATGNQQRPFVYGSLPGEEDYYFRSR"
     gene            complement(7394..>8165)
                     /locus_tag="VN968_23970"
     CDS             complement(7394..>8165)
                     /locus_tag="VN968_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXL32325.1"
                     /translation="VIGNLLDVPMEEREPLRDWSLAILGALEPVISAEAFARGNTAVK
                     DFLGYLEILVERRRAKPGNPERDVLTRLIQGEDSGERLSSKELLHNCIFLLNAGHETT
                     TNLIGNGLVALSGNPAQKKRLIDNPELIKTAVEEILRFESSNQLGNRMTTEHVELGGI
                     TLSAGTPVTLCIGAANRDPKQFTDPENLDIGRTPNRHLAFGTGAHQCAGMALARLEGA
                     IAISRFLARFPGYLLSGAPTRGGRVRFRGFLSVPCTVG"
BASE COUNT         1585 a         2494 c         2554 g         1532 t
ORIGIN      
        1 cgatgccggc ggctgccgcg tgtcgcgatc cggcataccg tcggtctcga cgatcaagtc
       61 gaaggcggca aaggcggcgt cgccgatatc agggaaaata gcggtgctcg ctatttcaag
      121 ctcaagctta acggcaatcc cgatgccgat accgcgcgcc tgatccgcat cggaagggaa
      181 ctcgccacgc tgtcatatga ctgcagcgtc acgctggacg ccaacgagca atacgccgat
      241 ctcaccgcgc tgggcgcgct ggtcgacagg ctcggcgctg acagcgcact caagccgatt
      301 gcggcaaagc tgttgtacat cgagcagccg atgccgcgcg atatcttcag gcaatcgccg
      361 ctcggcacgc tcgctgcctt cgatattatc atcgacgaag ccgacgattg ctacgacgca
      421 ttcccggtgg cgcgcgcgct cggctatcgc ggtatctcct ccaaatcctg caaggggacc
      481 tacaagtcga tcgtcaatac gacgcgtgcg gccaaatgga gcgcgggcgg cgagaagttc
      541 ttcatcacgg gcgaggacct gacctgtcaa gccggtctcg ctgtgcagca ggacctcgcg
      601 cttggcgcac tgatcggcgt cacccatgcc gagcgcaatg gccaccatta tgtcgacggc
      661 tttggcgaca cgcccgctgc cgaggcgcaa gcctttctcg ccgctcatcc cgatctctac
      721 gtcagcgacg ggaacaatat ccggctgtcg atccacgacg gcgacctcgt gacgggatcg
      781 ctggcagcgc cgggctttgc cacatccgtt caccccgagt ggacggcgtt gtcgccgctt
      841 caacaactaa aagcaaaaag tctgcaggag caatcaatat gacgacgcaa cgcctcggcg
      901 tgatcatgaa cggtgtcacc ggccgcatgg ggctcaacca gcatctgatc cgctcgatca
      961 tcgctatcag ggattccggc ggcgtgccgc tggccaatgg cgaccgcatg atgccggatc
     1021 cgatcctggt gggccgcgac tccgacaagg tcgagggtct cgccaggcgg ttcaatgtcg
     1081 aacgctggac caccgacctc gacaaggcgc tgctcgagcc gagcgacggc attttcttcg
     1141 atgccgcgac cacgcaggcg cgtccatcgc tgctgaccaa ggcgatcaac gccggcaagc
     1201 acgtctattg cgagaagccg atcgcgacca atctcgagga ggcagtcgcg gtgctcaggc
     1261 tcgccaacgc caagggcgtc aagcacggca ccgtgcagga caagctgttc ctgccgggtc
     1321 tgaagaaact ggcgttcctg cgcgattccg gcttcttcgg ccgcatgctc tcggtgcgcg
     1381 gcgagttcgg ctactgggtg ttcgaaggcg actggcagga ggcgcagcgg ccgtcatgga
     1441 attaccgcag cgaagacggc ggcggcatta ttctcgacat ggtctgccac tggcgttacg
     1501 tgctcgataa tctctttggc gaggtcgaaa gcgtcagctg tctcggcacc acggatatcc
     1561 cggagcgctg ggacgagaag ggcgagaagt acaaggcgac agcggacgat tccgcctacg
     1621 cgaccttcag gctcaagggc ggtgtgatcg cgcatatcaa catgtcctgg gtgacgcggg
     1681 tctatcgcga cgacctcgtc acctttcagg tcgatggcac ccacggctcg gcggtggccg
     1741 ggctttccga ttgcgtcatt caggcgcggc aagcgacgcc acggccggtg tggaatcccg
     1801 acgagaagcg cacccacgat ttctatgccg actggcagac gctgccggag aatgccgtct
     1861 acgacaacgg tttcaaagag cagtgggaaa tgttcattcg ccacgtctgc gaggatgcgc
     1921 cgtacagata tacgctgctg gaaggcgcca agggtgtgca gctcgcggaa tgcgcgctgc
     1981 agagctggcg cgagcggcgc tggatcgatg tcgctccgat caaggtgtag gggaacagga
     2041 ccatgaacaa accggttttg cccgtctcgt cgctgtcgct gaaattgccg aacgccgatc
     2101 gttcgatcga gacctatcgc ctcgcggcgt cgcggacgtt tccttcgaag ctcgaggaca
     2161 cgttgaaccg cgtggcgttt tccgcggccc atgtggtggc tgatccgctt gccgatgtcg
     2221 atccgtggct cacttcagct gttgactggg accggacgat tgcctttcgc gagcacgtct
     2281 gggatctcgg cctcggcgtc gccgaggcga tggataccgc gcagcgcggc atggggctgg
     2341 actggccgac ctcgctggaa ttgatccagc gctcggtcaa ggcagcgaag gcgaggggca
     2401 acgcgctggt gttttccggc gccggtaccg atcacctgga gcccagggat gcgaagacca
     2461 tcgacgacgt gatatcggcc tatgaggaac agatcgcagc cgtcgaaaaa gcgggcgggc
     2521 gcatcatcct gatggcgtcg cgcgcgctgg cgaaaatcgg ccgcaatccc gatgactacg
     2581 ccaaggtcta taaccgcgtg ctgtcgcagg tagcggagcc ggtgatcatt cactggctcg
     2641 gcgacatgtt cgatccggcg ctcagcgatt actggggcac ctcaagcctc gaccaggcga
     2701 tggatgtcgc ggtcggggtc atcaacgcca acgcgtcgaa ggtcgatggc gtcaaggtct
     2761 cgctgctcga caagcagcgc gagatcgaca tgcgccgccg gctcgacaac gatgtaaaaa
     2821 tgtataccgg cgacgatttt aattatgccg agctgatcgc cggtgacgac caaggctttt
     2881 cccatgcgct gctcggcatc ttcgatgcca tcgcgccggc ggcatcctac gcattgtcgc
     2941 ggctcgcggc gggcgacgag gccggcttcc acgacgtgtt gggaccgacg gtgccgctgt
     3001 cgcgccatat cttcaaggcg ccgacccggt tctacaagac cggcatcgtg ttcatggcct
     3061 atctcaacgg ccatcaggat catttcacca tggtcggcgg gcaggaaagc acgcggtcga
     3121 tactgcatct cgctgaattg tttcgtctgg cggatcaggc aggattgctt gccaatcccg
     3181 agttggcgac gcgccgcatg aaggccgtgc tcgcgacccg tggcgttgaa gcgtgatgcg
     3241 ggatttctcg aaagatcatc gctggctgtc attgaatacc gcgacggtcc gcaaacaggg
     3301 caatctggcc gaaatcatcg atgcctgcgc gcggcacgga atccgcgcca tcgatccctg
     3361 gcgcgatcag gtcgcgacca tcggtctcga tcgcgcggtg cgggcggtgc gcgacgccgg
     3421 gctcgaactg tccggttatt gccgcggcgg gatgttcaca tcggacgccg cgcatcggat
     3481 cgaagcacgc gacgacaatc gccgcaccgt cgatgaggcc aaggcgctgg gcgcgccctg
     3541 cgtcgtgctg gtggtcgggg gcttaccgca atattcgtgg ccgggcagtg tggcctcgaa
     3601 agacatcgcg gcggcgcggg cgcaggtcga cgacgccatc gccgaaatga tggtctatgc
     3661 gcgcgacgcc aacatgccgc tggcgatcga gccgctgcat ccggcctatg ccgccgaccg
     3721 cgcctgcgtg aatacgacaa agcaggcgct cgatatctgc gatcgcctcg atccgcaacg
     3781 cagcggcgcg ctcggcgtcg cactcgacgt ctatcacatc tggtgggacc cggaattgct
     3841 gccgcagatc gaacgcgcgg gcaaggatcg cctgctggca tttcacgtct gcgactggct
     3901 ggtgccgacc atggacattc taaatgaccg cggcatgatg ggcgacggtg tgatcgatat
     3961 taaatcggtg cgccgtgcgg tcgaggcgca aggctttgcc ggctattccg agatcgagat
     4021 tttttcctac gactggtggg aggagccgat ggatgaggtc ctgcagacct gcatcgcgcg
     4081 gcaccggtcg gtggtttgag ctaacctcat tctgcattcc ggcgttgccc ctgcatgcct
     4141 cgtctggctc ttgcgatgtt cgccccaacc ttgttgatat tggccccaga tgcctccggt
     4201 agccggaggt gccatcactg acgaacccgt cggacgcctg gagccaaggg aatgaccgat
     4261 ccatccgaat acacgccgcc caaagtctgg agctggagca aggagaacgg aggcaggttc
     4321 gcgaacatca atcgcccgat cgccgggccg acgcacgaca aggaactgcc ggtcggccac
     4381 caccccctgc agctttattc actggccaca ccgaacggcg tgaaggtcac ggtgatgctg
     4441 gaggaactgt tggcgctcgg ccatcgcggc gcggagtacg atgcgtggct gatccggatc
     4501 gacggcaatc agttcggcag cggcttcgtg gcgatcaatc cgaactccaa gataccggcg
     4561 ctgatggacc atagcgggcc gacgccgatc cgggtgttcg aatcaggctc gatcctgttg
     4621 catcttgctg agaagttcgg ggctttcctg ccaaccgacg tcgcgaagcg cgccgaatgc
     4681 ctgtcgtggt tgttctggca gatgggcagc gcgccttatc tcggcggtgg gtttggccac
     4741 ttctacgcct acgcgccgac caagatcgaa tacgcgatcg atcggttcgc gatggaggtg
     4801 aagcgccaac tcgacgtgct tgaccggcgt ctcgcggaca acgcgtatgt ggcgggcgac
     4861 gactattcga tcgcagacat cgcgatctgg ccctggtacg gcggtttggc gaagggcgtg
     4921 ctctatggcg cgggcgaatt cctgggcgtg caggagtaca aaaacctgca gcgctgggca
     4981 gatgccattg ccgctcgtcc ggccgtgcgg cgcgggcgaa tggtcaaccg cacctggggc
     5041 gagccctcaa gccagttgca cgaacgtcac gacgccagcg atttcgaaac caggacgcaa
     5101 gacaaggttg aggacggcca accggcgacc ggagcatgac gggcggcggc gtgatcgata
     5161 tcaaaccggt gcgccgtgcg gtcgaggcgc aagacatcac gccaggtccg aaaacgtttt
     5221 gccgcttgca aggtctcggc tgtggaaact aatatcggga gcgcgtctat cctacccatg
     5281 cgacctggag gaatggctga tgcgtcgctt tctgcgggca ctggcgatag ggcttacgct
     5341 tgtcgcactg cccggcgttg gctttgcggc gagcgacgaa gagacggtac gccttctcca
     5401 gcgcgacgcc gaccagggta acgccaatgc gcaagttcgt ctcggctatt tctacgaaaa
     5461 cggtcgcggt ggccttgcca aagacgatcg caaggctgca cgcctctata agctcgccgc
     5521 cgaccagggc aacgccgcgg cacgaaacaa tctcggcgtt ctttaccgag acggcggtgg
     5581 cggcctcacc aaagacgacc gcgaggctct gcgcctcttc aagctcgccg ccgatcaggg
     5641 caacgcttac gcgcagcaaa atctcggcgg cttctaccga gatggccgcg gcggtctcgg
     5701 caaggacgac cgcgaggctg tgcggctatt caagctcgcc gccgaccagg cgctcgccga
     5761 cgccagagcc agtctcggct atttctacga aaccggtcgc ggaggccttg ccaaggacac
     5821 ccgcgaggct gcgcgccttt accggctcgc cgtggatcag ggtaacgccg ccgcacaaaa
     5881 caatcttggc gtgttctatc gagatggcgg cggcggcctc accaaggacg accgcgaggc
     5941 tctgcgcctc ttcaagctcg ccgccgatca gggcaactcc tacggtcaac acaacctcgg
     6001 tggtttatat cgagacggct tgggtggcct tgccaaggac gagcgcgagg ctgcgcgcct
     6061 gttcaagctg gccgccgacc acggcaacgc tggcgccgga gttagtcttg gttacttcta
     6121 cgagaaaggc ctcggtgatc ttgccaaaga cgaccgtgag gctgtgcgcc tctacaggct
     6181 cgccgccgac cagggcaacg ccgccgggca aaataatctc ggcgttcttt acagggacgg
     6241 ccgcggtggc ctcaccaagg acgagcgcga ggccgcgcgc ctcttcaagc tggcctccga
     6301 tcagaacaac gcctatgggc aacaaaatct tggtattttc taccgggacg gtcgtggcgg
     6361 cctcaccatg gacaaccgtg aggccgcgcg cctcttcaag ctcgccaccg accaaggcaa
     6421 cgccaacgca cgaacagcgt tgaaccagat cactcaaccc tcgcaagagg taactgcggt
     6481 tgctaatgcc cgttcaaatc aacaggtggt cgaaccaagc tcggcgcctg ccgccaattt
     6541 gccgtctgtg ccggagcgcc gcgtggcgct tgtcattggc aatgccgctt atcaagctgt
     6601 gagcggattg gctaatacga ccaacgacgc cagagcgatt gccgatgtgc tcaaggccga
     6661 cggattcacc agcgtgcgaa tagctttgga tatgaccagg gcaagcatga ttgcctccct
     6721 caatgatttt caacgcgaag ctgacacatc ggattgggcc gtcgtctatt atgccggtca
     6781 cgggctggaa gtcagcggca cgaattatct cgtgccgatc gacgcaaaat tgggggacga
     6841 ccgggatatt caggatgagg cggtttcagt ggatcgaatt ctaagggcgg tcgaaaacgc
     6901 aaaaaagctc aggcttgtga tgctcgacgc atgtcgcgat aatccttttt tgaagaagat
     6961 gcatcgcagc atcgcgacac gctcgataag ccgcggactg gctgccatcg agcccctggg
     7021 tgccacactc atcgtgtttg ccgcgaagga tggcgagacg gcggaggacg gagccggcaa
     7081 ccacagtccg tttgcggcct cgcttatcga gcgattgcag cagccgacga ttgaaatcaa
     7141 caagcttttc cgcctcgtca ccagtgacgt tctaaaagct accggcaatc agcagcggcc
     7201 gttcgtctat ggatctcttc cgggcgaaga agattattat ttcaggtcga gatgaattaa
     7261 agcgtcggga cctgtttgat gcgcccacgc cctagagcgg gatgactttt cctcgaatcg
     7321 tcatcccgct ctatcttttt tgtttgagca tgatcttttc ggaaaaccgg tacccacttt
     7381 tccggatcat gccctagcca accgtacacg gcacgctcaa aaatccgcga aagcgaacgc
     7441 ggccgccgcg cgtcggcgcg ccgcttaaca ggtaccccgg aaatcgtgcc aggaatcgcg
     7501 aaatcgcgat ggcgccttca agccgcgcca gcgccatccc ggcgcattga tgcgcgccgg
     7561 tgccaaaggc aagatgccgg ttcggcgtgc ggccgatgtc aaggttttcg ggatcggtga
     7621 attgtttggg atcgcggttg gccgcaccta tgcacagcgt caccggcgta ccggccgaca
     7681 gtgtgatgcc gccgagctcg acgtgctccg tcgtcatgcg gttgccgagc tggttcgagc
     7741 tttcaaatcg cagaatttcc tcgacggcag tcttgatcag ctcaggattg tcgatcagcc
     7801 gttttttctg cgcaggattg cctgatagcg ccaccagtcc gttgccgatc aggttggtgg
     7861 tggtttcatg tccggcattg agcaggaaga tgcagttgtg cagcaattcc ttggacgata
     7921 gccgttcgcc actgtcctcg ccctggatga ggcgcgttag cacgtcgcgc tcgggattgc
     7981 cgggtttggc ccgccgccgc tcgaccagta tttccaggta gcccagaaaa tccttcaccg
     8041 ccgtgttgcc gcgggcaaaa gcttccgccg atatcaccgg ttcgagcgcg cccaagatcg
     8101 ccagcgacca gtcgcgtaat ggttcgcgct cctccatggg gacgtcgagc agattgccga
     8161 tcacc
//
LOCUS       DATNOI010000592         2684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_952075,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000592 DATNOI010000000
VERSION     DATNOI010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952075"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VN968_23975"
     CDS             <1..466
                     /locus_tag="VN968_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HXL32326.1"
                     /translation="EALVIEMVPKGSGSENNSWLRMAVPAEGVDAIKTFVIDCVLEAG
                     GKTCPPTIVGVGVGGTADLCVHLAKVAATRALGTHCDDPEGARLEEELGPAVNMLGVG
                     PQGLGGDSTAFAVHVETAATHITMNPVAVNMQCHSARRARATFRASGVEYGY"
     gene            479..1087
                     /locus_tag="VN968_23980"
     CDS             479..1087
                     /locus_tag="VN968_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HXL32327.1"
                     /translation="MAHYDFTTPLTEGAVRKLRLDDTVSLNGTLFGIRDATQIHMFDR
                     GRKTRFDLAGHAVIHTAPNVKKVQKSAAHPAGYAPLCVGTTTSDRMERFTRPLMQQCG
                     VRLVIGKGGLREESLKAFGDLGGAYLAIIGGTAALETTWVESIEDVDLDDLNPESLWK
                     FRVRNFGPLLVAMDSHGGSLYSKVKRTAQERRAAALRRLGVR"
     gene            1116..1946
                     /locus_tag="VN968_23985"
                     /pseudo
     CDS             1116..1946
                     /locus_tag="VN968_23985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
     gene            complement(1910..2551)
                     /locus_tag="VN968_23990"
     CDS             complement(1910..2551)
                     /locus_tag="VN968_23990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXL32328.1"
                     /translation="MRLLTRVVALLMSALALAAQAQNLSIATGGTGGVYYPLGGGMAA
                     VLSKYVPGMQATAEVTGGSVANLQLIGTGKPYLGMTMADATLDAYKGQDKFTGKPVPV
                     RTLMVMYPNRMHVVSIEGAGVNKIADLKGKRVSTGSGGSATEVMAFRVIEAAGLDKDR
                     DMRRERLGVAESVNAIKDRKIDAFFWFSSLKFSASLWTRTRSSLWSKTALTML"
BASE COUNT          472 a          876 c          907 g          429 t
ORIGIN      
        1 ggaggcgctg gtgatcgaga tggtgccgaa gggctcgggt tccgagaaca actcgtggct
       61 caggatggca gtccccgccg aaggcgtgga cgccatcaag acgttcgtga tcgattgcgt
      121 gctggaggcc ggcggcaaga cctgcccgcc gaccatcgtc ggcgtcggcg tcggcggcac
      181 cgcggacctt tgcgtgcatc tcgcgaaggt cgcggccacc cgggcgctcg gcacgcattg
      241 cgacgatccc gagggggcca ggctggagga agagctcggc ccggcggtga acatgctggg
      301 cgtcggcccg caagggctcg gcggcgattc caccgccttc gcggtgcacg tcgagacggc
      361 ggccacccac atcacgatga acccggtggc ggtgaacatg cagtgtcact cggcgcgccg
      421 cgcgcgcgcc actttccggg cttcgggcgt cgagtatggg tactagagct gctttcgaat
      481 ggcacactac gacttcacga cgccgctgac cgaaggcgcg gtgcgcaagc tgcgcctcga
      541 cgacaccgtc agcttgaacg gcacgctgtt cggcatccgc gacgcgacgc agattcacat
      601 gttcgaccgg gggcgcaaga cgcgcttcga cctcgcgggc cacgcggtga tccataccgc
      661 gccgaacgtg aaaaaggtgc agaagagcgc cgcgcatccg gcgggctacg cgccgctttg
      721 cgtcggcacc accaccagcg accgcatgga gcgcttcacg cggcccctga tgcagcagtg
      781 cggcgtgcgg ctggtgatcg gcaagggcgg cttgcgcgaa gaatcgctca aggccttcgg
      841 cgatctgggc ggcgcctacc tcgccatcat cggcggcacc gcggcgctgg agacgacctg
      901 ggtcgagtcg atcgaggacg tggacctcga tgacctcaat cccgaatcgc tgtggaagtt
      961 tcgcgtcagg aacttcggcc cgctgctggt ggcgatggac agtcacggcg gcagtcttta
     1021 ctcgaaagtg aagcggacag cgcaggagcg ccgcgcggcg gcgctgcgcc gactcggcgt
     1081 gcggtagaat cgccgcacaa cccgaggagg agtccatgcg atttttgacg cgcgtcggcg
     1141 tgcttttgat ctcggcgttc gcgttcgcgc tggtcgcgca ggcgcagaac ctttcgattg
     1201 ccaccggcgg aaccggcggc gtgtattacc cgctgggcgg cggcatggcg gcggtgctct
     1261 ccaaatacgt tccgggcatg caggccacgg ccgaggtgac cgggggctcg gtggccaacc
     1321 tgcagctcat cggcaccggc aagccctatc tgggcatgac catggcggat gcaacgctcg
     1381 atgcgtacaa gggccaggac aagttcaccg gcaagccggt cccggtgcgc acgctcatgg
     1441 tgatgtatcc gaaccgcatg cacgtcgtct cgatcgaggg cgccggcgtt gccaagatgg
     1501 cggacctgaa aggcaagcgc gtgtccaccg gatcgggcgg cagcgcgacc gaagtcatgg
     1561 cgttccgcgt catcgaggcc gccggcctcg acaaggacaa ggatatgcgg cgcgagcgcc
     1621 tcggcgtcgc cgagtcggtg aatgcgatca aggaccgcaa gatcgacgcc ttcttctggg
     1681 tcggcggcct gccgaccgcg gcgatcaccg acctcgccag cacgccgggc gtgaagatca
     1741 agctgatcga ccatgccgac ctggtgccgg cgatgaacag aaaatacggc gagctctatg
     1801 tgcaggacgt gattccgaag gacatgtacc gcggcatgga cgccgacaac aggcaggcca
     1861 cggtgatgaa cctgctggtg gcgcaccaga acatggacga gaagaccgcc tatagcatcg
     1921 tcaaggcggt cttcgaccac aaggaagacc tggtccgcgt ccacaaggaa gcggagaact
     1981 tcaggctcga gaaccagaaa aaggcgtcga tcttcctgtc cttgatcgca ttcaccgact
     2041 cggcgacgcc gaggcgctcg cgccgcatat ccctgtcctt gtcgaggccg gcggcctcga
     2101 tgacgcggaa cgccatgact tcggtcgcgc tgccgcccga tccggtggac acccgcttgc
     2161 ccttcaggtc ggcgatcttg ttgaccccgg cgccctcgat cgagacgacg tgcatgcgat
     2221 tcggatacat caccatcagg gtgcgcaccg ggaccggctt gccggtgaac ttgtcctggc
     2281 ccttgtacgc atcgagcgtc gcgtccgcca tggtcatgcc cagatagggc ttgccggtgc
     2341 cgatgagctg caggttggcc accgagcccc cggtcacctc ggccgtggcc tgcatgcctg
     2401 gaacgtattt ggagagcacg gccgccatgc cgccgcccag cgggtagtac acgccaccgg
     2461 ttccgccggt ggcaatcgaa aggttctgcg cctgcgctgc cagcgcgagc gccgacatca
     2521 aaagcgcgac gacgcgcgtc aacaatcgca tggactcctc ctctggttgt gcggcgattc
     2581 taccgcacgc cgagtcggcg cagcgccgcc gcgcggcgct cctgcgctgt ccgcttcact
     2641 ttcgagtaga ggctgccgcc gtggctgtcc atcgccacca gcag
//
LOCUS       DATNOI010000593        10435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1487002,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000593 DATNOI010000000
VERSION     DATNOI010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10435
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487002"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VN968_23995"
     CDS             complement(<1..407)
                     /locus_tag="VN968_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXL32329.1"
                     /translation="MPINADYRGPGDLPEVIPVFPLPGALLLPRGQMPLNIFEQRYLA
                     MVDDALRDGHRLIGMIQPDVSHSQNEARPALFRVGCVGRITQLAEAGDGRYILELTGI
                     ARFKVVEEISALTAYRQCKVDYFPYIDDFTARKG"
     gene            complement(492..1415)
                     /gene="trxA"
                     /locus_tag="VN968_24000"
     CDS             complement(492..1415)
                     /gene="trxA"
                     /locus_tag="VN968_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXL32330.1"
                     /translation="MTIVEQGSVPAPQAAPDLIKETTTQTFVKDVIEESKRQPVLIDF
                     WAPWCGPCRQLTPIIEKAVRAAKGKVKLVKMNIDEHPAIPGQMGIQSIPAVIAFVNGQ
                     PADGFMGAVPESQVTAFIDKLTAGMPAAGEPNIAEILKEAEAVLAEGDPATAASIYAE
                     VLAADATNIAGLAGLAKCYMATGAIEQAKQTIAMVPESKRNDAGVKAVQASIDLAEQA
                     KALGPVTELEQRVAANPLDHQARFDLATALNASGKRAEATNQLLEIVKRDRKWNEDGA
                     RKQLVQFFEAWGPTDEATVDGRKRLSTILFS"
     gene            1902..1976
                     /locus_tag="VN968_24005"
     tRNA            1902..1976
                     /locus_tag="VN968_24005"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1934..1936,aa:Gly,seq:gcc)
     gene            2178..4457
                     /locus_tag="VN968_24010"
     CDS             2178..4457
                     /locus_tag="VN968_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HXL32331.1"
                     /translation="MSRKIVLLSDGTGNSAARVWRTNVWRTFEALDLSGNDQVAFYDD
                     GVGTSTFKPWAILGGAFGFGLKRNVIDIYKFACRNYKDETDDIYGFGFSRGAFTIRVV
                     IGLILNQGLVSAANEAELDKKAIAAYRKYRSERYHTLWPWHPEDWYRAIRNALSPIEY
                     DKRDNRQVKHIRFVGVWDTVAAYGLPIDEMTRGVSRWIVPLELPTHTLDRARVMRACQ
                     ALSLDEERTTFHPELWDEKIVPPSEFDPDQKRHIADEQISQVWFAGVHSNVGGGYPDD
                     ALAYIPLVWMLTEAKRCGLKLKSDVGDPPADPDALKNAISMADKDGRIYDPRAGLGGY
                     YRYGPRKLVQLCNYKYSKKEDDEVTIERPKIHETVFRRIGNHAHAYAPIGLPATYDIV
                     KSNGEIVTPDQYGFETSDAAKARADAQEHIWNEIWKRRIVYFATVGATAWLLAFPLIS
                     GAQRADEFQSPVRWISDIIRLVGAFLPGFASTWVDGYARAPISFVLLALLVASLTIWG
                     MKIAARISDRMGAIWRKTPGAPSGLPDDGIYRLRSNRIYIWFHEGLKRRWAPALFALL
                     FIFLGLSLLSHGLYDVQDVAGLTCKESSNATGLAKGWTNPKTDPESRTVEFKTSDLCK
                     PTGILLESRGARYHVEVESTEPWNDGGTPVLVGGFSANQPPVWYHRILLGLGVPLRRE
                     LTQDWFRIVLRYGGVGGEEVFLDPDPQDSKIEANIKPTREGELFIFVNHAVIGIPGLY
                     DFFYRNNGGGGKIKVTRSY"
     gene            complement(4485..5291)
                     /locus_tag="VN968_24015"
     CDS             complement(4485..5291)
                     /locus_tag="VN968_24015"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXL32332.1"
                     /translation="MSAVWRDPTPLRRSFAERGATPIAGDIASPERWAGRLPRVDAVI
                     HAASDFSSDMGAIDRRLLDVLLPSLAAQPKRPRFIYTGGCWLFGATGDDVATEETPLR
                     PLPAFAWMVPHLRQILAAPDIDGIVIHPAMVYEPDGCVFLRFARDALERDAIRVVESG
                     QVRWPLVHSEDLATLYALALRDAPAGSSYIGAAIEGLPVGRIASAFARRFGTRRQMPD
                     IISAEALTAELGAWTRGYALDQQLSGAKARRVLGWRPKHLDPEGEIARLP"
     gene            complement(5305..5412)
                     /locus_tag="VN968_24020"
                     /pseudo
     CDS             complement(5305..5412)
                     /locus_tag="VN968_24020"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5625..6887
                     /locus_tag="VN968_24025"
     CDS             5625..6887
                     /locus_tag="VN968_24025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXL32333.1"
                     /translation="MQSVFNRSVLACAAILTIVGISQAAAQQKPLKKYESGAKEFWTH
                     PPDDWFLGDETEGQKGLAPPSGPPTGSSEAELDAMMKKIKLPQGFKIEVYAPGVLAAR
                     QMAWGDKGTLFVGSFGLGNVYAIKDNGGKKEVKTILKGLNMPTGLAFKDGALYVIAVD
                     KLIKYENAEANLDKLGDGKVVYDDMPSYAAHGWKYLAADKDGWFYVPFGPPFNIGIPP
                     TSVSQIRRVDPKTGNAEVWALGVRNSVGGDVDPRSGKYWFTENARDWISDDLPSDKLN
                     MISKIGEHFGYPYCHQGNLPDTKFAMGHKCSEFTPPVLNLGAHVAPLGMKFYTGSQFP
                     SEYKNSILIAEHGSWNRHKYQGARIVRVTVGPDGKNPKQDVFASGWIEGDQGYLGRPA
                     DIIQDKDGSILVADDWAGAIYRISYSKK"
     gene            7112..7693
                     /locus_tag="VN968_24030"
     CDS             7112..7693
                     /locus_tag="VN968_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXL32334.1"
                     /translation="MRSALFGMLSVAMTCGSFAHAADIAAGKAKAELCIGCHGEGGIS
                     QTENIPSLAGQPDQFIQWQLVFFRSGTRKNEQMQPIVEQINNEDIRNLGAYFASLTPP
                     KPTKPDDNPDLSTKGAQAAAGRRCASCHTDTFAGTKAVARIAGQREEYLLKALHDYKS
                     SVRSGGGMAAMADVAYPLSEEEIEALAHYLAHL"
     gene            complement(7841..8764)
                     /gene="panE"
                     /locus_tag="VN968_24035"
     CDS             complement(7841..8764)
                     /gene="panE"
                     /locus_tag="VN968_24035"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXL32335.1"
                     /translation="MRILVVGAGAIGGYFGGRMLQAGRDVTFLVRPRRAAELADAGLV
                     IKSPNGDVTLKDPPALQADRLSEKFDVVLLSCKAFDLGDAIKSFASAVGPQTSIIPLL
                     NGILHLDVLDGKFGPERVLGGLCAIAATLNDKREVVQLNPLQSLNFGERDGRLSDRVR
                     AIAEAMASGNFGSVASENIVQEMWEKWVFLATLAACTCLMRASVGNILASPGGKDFIL
                     GMLDECSAVAAAEGHDPRGPFFERTRGMLTAEGSQMTASMFRDIKAGAPVEADHVIGD
                     LVARGDAAKVPVPKLRTAYTHLKAYEKQREN"
     gene            complement(8852..9589)
                     /locus_tag="VN968_24040"
     CDS             complement(8852..9589)
                     /locus_tag="VN968_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL32336.1"
                     /translation="MEKRNAAVAVIGAGDYIGTEIAKKFAAEGFTVFAGRRDGAKLEP
                     LVRDIEAAGGSIVARALDAREADEVAAFLNDADKHAPLEVCIFNVGANVNFPVLETTD
                     RVFRKVWEMACWAGFVAGRESARLMLPHGKGNIFFTGATASLRGGSGFAAFASAKFGL
                     RAVAQAMARELGPKNIHVAHLVIDSSVDTAWVRERREQLWGKEALDNPDLLMPPASVA
                     ASYWQLYQQPRSAWTFELEIRPSGEKW"
     gene            complement(9647..10255)
                     /locus_tag="VN968_24045"
     CDS             complement(9647..10255)
                     /locus_tag="VN968_24045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HXL32337.1"
                     /translation="MTQTSPQFLFDVGSPNAFLSHEAIPGIEQRTGVKFEYVPILLGG
                     IFKATNNKSPAETLAGIKNKPEFQVLETERFVKRFGVKPYTMNPFFPVNTLNLMRAAV
                     AAQFEGVFEKYVDAAFHHMWGEPKKMDDPEVAMKAFTASGLDAAKLFARAQEGDVKAK
                     LIENTQAAVERGAFGSPTFFVGKEMFFGKEQLREVEELVSGK"
BASE COUNT         1991 a         3266 c         3169 g         2009 t
ORIGIN      
        1 cccttgcgcg cggtgaaatc gtcgatatag gggaaataat ccaccttgca ttgccgatac
       61 gcggtcagcg ccgagatttc ctcgaccacc ttgaagcgcg cgatgcctgt gagttcgaga
      121 atgtagcggc cgtcgccggc ctcggcgagc tgggtgatgc gcccgacgca gccgacccga
      181 aacagcgccg gcctggcttc gttctgcgag tgggaaacat ccggctggat cattccgatc
      241 agccgatggc cgtcgcgcag cgcgtcgtcc accatcgcca gatagcgctg ctcgaagatg
      301 ttgagcggca tctgaccgcg cggcaacagc agcgcgccgg gcagcggaaa caccgggatc
      361 acctcgggaa ggtcgccggg cccgcgataa tcggcattga tcggcatgtg cggttccggc
      421 gctaatccag agtttctaaa cttgcgcgca tcccaatcgc aaaaccggcg tccacgcttg
      481 cggaacacgc gctatgagaa caagattgtc gatagccgct ttcgtccatc cacggtggcc
      541 tcatcggtcg gaccccacgc ttcgaagaac tgcaccagct gctttcgcgc gccatcctca
      601 ttccatttac ggtcgcgctt gacgatttcg agcaactggt tggtggcctc ggcgcgcttg
      661 ccgctggcgt tgagcgcggt cgcaagatcg aatcgtgcct gatgatccag cggatttgct
      721 gcgactctct gctccagctc ggtcaccggg ccgagcgcct tggcctgctc ggcaagatcg
      781 atcgacgcct gcacggcctt tacacctgca tcattgcgtt tcgattccgg caccatcgcg
      841 atggtttgct tggcctgctc gatggcgccg gtcgccatgt agcatttcgc gagccctgcg
      901 agccccgcga tattggtggc gtccgcggcc agcacctcgg catagatcga tgccgcggtg
      961 gcgggatcgc cctcggccag aacggcttcg gcctccttga ggatttccgc gatattgggc
     1021 tcgcccgcgg ccggcatgcc cgccgtaagc ttgtcgatga aggcggtgac ctggctttcc
     1081 gggaccgcgc ccatgaaacc atcggcgggt tggccgttga cgaaagcgat cacggccggg
     1141 atcgactgga tacccatctg gcccggaatc gccggatgtt cgtcgatgtt catcttgacc
     1201 agcttgacct tgccctttgc tgcccggacc gccttctcga tgatcggcgt gagctggcgg
     1261 caggggccgc accacggcgc ccagaagtcg atcagcaccg gctggcgctt cgattcctcg
     1321 atgacgtcct tcacgaacgt ctgggtggtc gtctccttga tcaaatcggg cgccgcctgc
     1381 ggcgccggca cgctgccctg ttctactatc gtcacgggaa tcctcgcctg atgtatcggg
     1441 atcgggccta ttctagcacg ccccagggtg gtggttgaga agttctctgc agctaagtgg
     1501 cggtttgccg ggaaatttca atcggttcca ggtgcggtcg gaagcctcgg atggatcggc
     1561 cgcgatcgat ggcgtccggc ccttcgtcga atggtgggcg gcctttgatg gcgacgcgtc
     1621 ccgtcggtga cgaaccggcc gggacccttc ggggctggtc cggcaaatgg ctcgaatcgc
     1681 tccggagtac gctgggcagc cggccttccg ggttcccgga agccgcgacc agtgaggtcg
     1741 gcgaccgttc gcaggccgtt tcgggctgat ttgcccgcaa tcgctccgat atcggacgat
     1801 tgggccggtt cgaagaccgc gcggaggctg ttgcattcac ggtgcgcatt tggcatagct
     1861 ctgcccgttg tcggcgcgcg atcgccgcca atctctcgga tgcgggtgta gctcagtggt
     1921 agagcacgac cttgccaagg tcggggtcga gggttcgaat cccttcgccc gctccagaat
     1981 tttttgagtg gcaaagcaat ggtctaacga caagccgccg cccggcggct ttgttgttct
     2041 acgtccattc atttacaacc gtcggcctgc gcgattcctg attaccgatt gtgaaaattt
     2101 tcatactcgg ggagcggcgt gcaatgttat aaaaccatag gttgtaaagc cgtcgatgac
     2161 gcggcggggg ggattccatg agtcgaaaaa ttgttctgct gtcggacggc acgggcaatt
     2221 ccgcggcgag ggtctggcgc acgaatgtct ggcgcacctt cgaggcgctg gacctgtcgg
     2281 gaaatgacca ggtcgcattc tacgacgacg gcgtcggcac ctcgacgttc aagccgtggg
     2341 cgattcttgg cggcgcgttc ggctttggcc tgaagcggaa cgtgattgac atctataaat
     2401 tcgcctgccg caactacaag gacgagaccg acgatatcta tggcttcggc ttcagtcgtg
     2461 gcgccttcac gatccgcgtg gtcatcggcc tgattctcaa tcagggcctg gtgtctgctg
     2521 ccaatgaagc cgagctcgac aaaaaggcga tcgccgccta ccggaagtat cgcagcgagc
     2581 gttaccatac gctgtggccc tggcatcccg aggactggta ccgggcaatt cggaacgcgc
     2641 tatcgccgat cgagtacgac aagcgcgaca atcgccaagt caagcacatc cgcttcgtcg
     2701 gggtgtggga taccgtcgcc gcttacggtc tgccgatcga cgagatgacc cgcggcgtca
     2761 gccgctggat cgtgccgctc gaacttccca ctcacacgct cgatcgcgca cgcgtgatgc
     2821 gagcctgtca ggcgctgtcg ctcgatgaag agcgcaccac gttccatccc gagctgtggg
     2881 acgaaaaaat cgttccgcct tcggagttcg atccggacca aaagcgtcac atcgccgatg
     2941 agcagatcag ccaggtgtgg ttcgccggtg tccattccaa cgtcggcggc ggttaccccg
     3001 atgacgcgct ggcctatatc ccgctggtct ggatgctcac cgaggcgaaa cgctgcggcc
     3061 tcaaactcaa gtctgatgtt ggggatccgc ccgccgatcc cgatgcgtta aaaaatgcca
     3121 tctcgatggc tgacaaggac ggacgaatct acgatccgcg tgcgggactc ggcggctatt
     3181 accgctacgg cccgcgcaag ctggttcagc tctgcaatta caaatattcg aaaaaggaag
     3241 acgacgaagt cacgatcgag cgtccgaaaa tccacgaaac cgtcttcagg cggatcggaa
     3301 accatgcgca cgcctacgcg cccatcgggc tgcctgccac ctacgacatc gtcaagagca
     3361 acggcgagat cgtcacgccc gatcaatatg gcttcgagac cagcgacgcc gcaaaggccc
     3421 gcgcggacgc gcaggaacac atctggaatg aaatctggaa acgccgcatc gtctatttcg
     3481 caaccgtcgg cgctaccgca tggttactgg cgttcccgct gatcagtgga gcgcaacgcg
     3541 ccgacgagtt ccagagcccg gtccggtgga tctcggacat cattcgcctc gtcggcgcgt
     3601 tcctgccagg cttcgcctcg acctgggtcg acggctatgc ccgtgcgccg atctccttcg
     3661 tcttgctggc tctgctcgtg gcctccctca ccatatgggg aatgaaaatc gctgcgcgta
     3721 tctcggatcg catgggcgcg atctggcgaa aaacgcctgg cgcgccatcg ggtttgccgg
     3781 atgacggcat ttaccgtctg cgcagcaatc ggatctacat ctggtttcac gagggcctga
     3841 agcgccgctg ggcgccggcc ctgtttgcgc tgctgttcat ctttctcggc ctctcgcttc
     3901 tcagccacgg cctctacgat gtccaggacg tggcgggcct gacctgcaaa gagagcagca
     3961 acgccacggg ccttgccaag ggctggacaa atcccaagac ggatccggaa tcgagaacgg
     4021 tcgagttcaa gacctcggat ctttgcaagc cgacgggcat tcttctggaa agcagaggtg
     4081 cgagatatca tgtggaggtc gagtcgacgg aaccctggaa cgacggtggc accccggtcc
     4141 tggttggcgg attctccgcc aaccagccgc cggtctggta tcatcggatt ttactcggac
     4201 tcggcgtgcc gctgcgacga gaactgacgc aggactggtt tcgaatcgtg ctgcgttacg
     4261 gcggcgtcgg cggtgaagag gtttttctgg atcccgatcc ccaggattca aagatcgaag
     4321 ccaacatcaa acccacccgc gagggcgaac tcttcatctt cgtcaaccac gctgtcatcg
     4381 gcattcccgg gctctacgat tttttctacc gcaacaatgg cggcggcggt aaaataaagg
     4441 tgacgcgaag ctattaacag cggcggggcc cgatccctgc gccatcacgg cagccgcgcg
     4501 atttcacctt ccggatcgag atgcttcggc ctccagccga gaacgcgccg ggctttcgcg
     4561 ccgctaagct gctggtcgag cgcgtagccc ctggtccagg cgccgagttc ggccgtcagc
     4621 gcttcagcgg aaatgatgtc cggcatctga cgccgcgtgc cgaaacgcct tgcaaacgcg
     4681 ctggcgatgc ggccgacggg aaggccttcg atcgcggcgc cgatatagct tgaccccgct
     4741 ggcgcgtcgc gaagcgccag cgcgtagagg gttgcgaggt cttcgctgtg cacgagcggc
     4801 catcgtactt gcccgctttc caccacgcgg atcgcatccc gttcgagggc atcccgggca
     4861 aagcgaagga agacacagcc gtcgggctcg tagaccatcg ccgggtggat gacgatcccg
     4921 tcgatgtcgg gtgcggcgag gatttgccgc agatgcggca ccatccacgc gaaagcaggc
     4981 aggggacgaa gcggcgtctc ctcggtcgcg acatcgtcgc cggtcgcgcc gaacagccag
     5041 cagccgccgg tatagatgaa gcgcggcctt ttcggctgcg cggccaggga cggcagcagc
     5101 acgtcgagca ggcgacgatc gatcgcgccc atgtcgctgc tgaaatcgct ggcggcatgg
     5161 ataacggcat cgacacgtgg aagcctcccg gcccatcgct cgggcgatgc gatatcgccg
     5221 gcgatcggcg tcgcgccacg ttcggcaaag cttcgccgca gcggcgtcgg atcgcgccag
     5281 accgcagaca ttatgtccgc acctgatgag ttctcgcacc acggctgagc cgatcgagcc
     5341 cgtcccaccc aaaaccaaaa cgcgcacgag ccagctcctt aacctgatgg ttgcctgatc
     5401 cggcgaccgc acggttgcga cgccccgaca tgttgcggcg cggcgggcac cagcttattt
     5461 gttttcgcct gacaggtccg cgcggcggcg cttctctccc gcgtcggatg tgataacgtt
     5521 ttgaggtctg atccaccccg ccttcactgc aaacccaccc aagttcatct cgaaacgttc
     5581 aaaaataaaa gcgcagccgc atgggctgct tagggagtga cgtaatgcaa tcggtattca
     5641 atcgatcggt gcttgcgtgc gccgcgattc tcaccattgt ggggatttca caagcggccg
     5701 cacagcaaaa acccctgaaa aaatacgagt ccggcgccaa ggaattctgg acccacccgc
     5761 ccgacgactg gttcctcggc gacgagaccg aaggccaaaa gggcctcgca ccgccatcgg
     5821 gtccgccgac cggctcttcc gaagccgaac tcgacgcgat gatgaagaag atcaagctgc
     5881 cgcaggggtt caaaatcgaa gtctatgcgc cgggcgtgct ggccgcgcgg cagatggcct
     5941 ggggcgacaa gggcacgctg ttcgtcggtt cgttcggtct cggcaatgtc tacgccatca
     6001 aggacaatgg cggcaagaaa gaggtcaaga ccatcctcaa gggcctgaac atgccgaccg
     6061 gcctcgcgtt caaggacggc gcgctgtacg tcatcgcggt cgacaagctg atcaaatacg
     6121 agaacgccga agccaatctc gacaagctcg gcgatggcaa ggtggtatat gacgacatgc
     6181 cgtcctacgc cgcgcatggc tggaagtacc tcgccgccga caaggacggc tggttctatg
     6241 ttccgttcgg acctcccttc aacatcggca tcccgccgac cagtgtgtcg cagatccgcc
     6301 gcgtcgatcc caagaccggc aacgccgaag tctgggcgct cggcgtccgc aacagcgtcg
     6361 gtggcgatgt cgatccgcgc agcggaaaat actggttcac ggaaaacgcc cgtgactgga
     6421 tcagcgatga cctgccgagc gacaagctca acatgatctc gaagatcggc gagcacttcg
     6481 gctatcccta ctgccaccag ggcaacttgc cggatacaaa gttcgcgatg ggtcacaagt
     6541 gttcggaatt caccccgccg gtgctcaatc tcggtgcgca tgtggctccg ctcggcatga
     6601 agttctatac cggaagccag tttccctccg agtacaagaa ctcgatcctg atcgccgaac
     6661 acggctcgtg gaaccggcat aaatatcagg gcgctcggat cgtgcgcgtg accgtcggtc
     6721 cggacggcaa gaatcccaag caggacgtgt tcgcatccgg ctggatcgaa ggcgaccagg
     6781 gttatctcgg ccgcccggcc gatatcatcc aggacaagga tggttcgatc ctcgtcgccg
     6841 acgactgggc cggtgcgatc tatcgcatca gctacagcaa gaagtaggtc tcgatcatca
     6901 gaggctgcgg ctgctccaac aattggaacc agccgcagct ttattatttg gagtacgtgg
     6961 cgatccaact tcggtcgtca tgcccggact tgatccgggc atccatcaaa acgaaaacat
     7021 ctggcgagaa agatggattg ccgggcatag gcgagcggaa gcgacgccgt ccttcggacg
     7081 gctatgcccg gcaatgacgg gagaggttgt gatgcgtagt gcgttgtttg gaatgctgtc
     7141 agttgcgatg acttgcggat catttgccca cgccgccgac atcgccgccg gcaaggcgaa
     7201 agccgaatta tgtatcggct gccacggcga agggggtatt tcgcaaaccg agaacattcc
     7261 ctcgcttgca ggccagcccg atcagttcat tcagtggcag ctggtgttct ttcgcagcgg
     7321 cacccgcaag aacgagcaga tgcagccgat cgtcgagcaa atcaacaacg aggatattcg
     7381 caatctcggc gcctattttg cctcgctcac cccgcccaaa cccacaaaac ccgacgacaa
     7441 tcccgattta tccaccaagg gcgcgcaggc tgccgcgggt cggcgctgcg cctcatgcca
     7501 caccgataca tttgccggca ccaaggccgt cgcccgcatc gcgggccagc gcgaggaata
     7561 tctgctcaag gcgctgcatg attacaagtc gagcgtgcga tccggcggcg gcatggcggc
     7621 gatggcggat gtcgcctatc ccctgagtga agaggaaatc gaagccctgg cgcattatct
     7681 cgcgcacttg tgaaatgcga taagacgccc accaaaatcg tcacgcccgg gcatccacgt
     7741 cttcctcggc tgagaaaagc caagacgtga atggccggga cgtctagcgc gaagacgcgc
     7801 ttcgcgcttc tgcccggcca tgacgaacgc cgttggtgtc ttagttttcc cgctgcttct
     7861 catacgcctt cagatgcgtg taggcggtgc gcagttttgg caccggcact ttcgcggcgt
     7921 cgccgcgtgc gaccaggtcg ccgatcacat gatcggcttc gacgggtgcg cccgctttga
     7981 tgtcgcggaa catggatgcc gtcatctgcg agccttccgc ggtgagcatg ccgcgggtcc
     8041 gctcaaagaa cgggccgcgg gggtcatggc cctcggccgc cgcgacggcg ctgcattcat
     8101 cgagcatgcc gaggataaaa tccttgccgc ccggcgacgc caggatattg ccgaccgaag
     8161 cccgcatcag gcaggtgcag gctgcgagcg tggcgagaaa aacccatttc tcccacatct
     8221 cctgcacgat attttcgctg gccaccgagc cgaaattgcc gctcgccatc gcttctgcaa
     8281 tggcgcggac ccggtccgac agtctgccgt cgcgttcgcc gaaattgagc gactgcaacg
     8341 gattgagctg cacgacctcg cgcttgtcgt tgagcgtggc cgcgatggcg cagagaccgc
     8401 cgagcacgcg ctcgggtccg aatttgccgt cgagcacgtc gaggtgcaga atgccgttga
     8461 gcagcgggat gattgacgtc tgcggtccaa ccgccgatgc aaacgacttg atcgcatcgc
     8521 cgaggtcgaa cgccttgcag ctcagcaaga ccacgtcgaa tttttcgctt aatctgtcgg
     8581 cctgcaatgc cggtggatct ttcaacgtca cgtcgccatt cgggcttttg atcacgaggc
     8641 cggcgtcagc cagctctgcc gcgcgcctgg ggcggaccag aaaggtcaca tcgcggccag
     8701 cctgcagcat cctgccgccg aaatatccgc cgatggcgcc ggcaccgacc acgagaatgc
     8761 gcatttgttg agttcctttg ttaagggcga gtagggagtg gcgaatagcg aatagggaag
     8821 gtcccactcg ctatccgcta ttcgcgtcct cttaccactt ttccccggac gggcggatct
     8881 ccagctcaaa ggtccaggcg ctgcgcggct gctggtaaag ctgccaataa gacgcggcga
     8941 ccgacgccgg cggcatcagc aagtccggat tgtcgagcgc ttccttgccc cagagctgct
     9001 cccggcgctc gcgcacccag gcggtgtcga cgctggaatc gatgactaga tgcgcgacat
     9061 ggatgttctt cggcccgagt tcgcgcgcca tcgcctgcgc caccgcccgc aagccgaatt
     9121 tggcgctggc gaaggccgca aagccgctgc cgccgcgcaa gctcgcggtc gccccggtga
     9181 agaagatgtt gcccttgccg tgcggcagca tcagccgtgc cgattcgcgg ccggcgacaa
     9241 agccggccca gcaggccatt tcccagacct tgcggaagac gcggtcggtg gtctccagta
     9301 caggaaagtt gacgttggcg ccgacattga agatgcagac ctcgagcggc gcatgcttgt
     9361 cggcgtcatt gaggaacgcc gccacctcgt ccgcctcacg cgcgtcgagc gctcgcgcga
     9421 caatcgatcc gcccgcggcc tcgatatccc gaaccagcgg ctcgagcttg gcgccgtcgc
     9481 gccgcccggc gaatacggtg aatccctcgg cggcgaattt cttggcgatt tcggtgccga
     9541 tataatcgcc ggcgccgatc accgcgacgg ctgcgttgcg tttttccatt tggggttctc
     9601 caccaagccc gacgagccta cgcgtcctcc tggcaatgac ggcaagtcac ttacccgaaa
     9661 ccagctcctc gacctcgcgc aactgctcct tgccgaaaaa catttccttg ccgacgaaga
     9721 aggtcggcga gccgaaggcg ccgcgctcga ccgccgcttg ggtgttctcg atcagcttcg
     9781 ccttgacgtc cccttcctgg gcgcgggcga acagttttgc tgcgtcgagg ccggatgccg
     9841 tgaacgcctt catcgccact tcgggatcgt ccattttttt cggctcgccc cacatgtgat
     9901 gaaacgcggc gtcgacatat ttctcaaaca cgccctcgaa ctgggctgcg accgcggcgc
     9961 gcattagatt gagggtgttg accggaaaga acgggttcat cgtgtagggc ttaaccccga
    10021 aacgctttac gaaccgctcg gtctccagca cctgaaactc gggcttgttc ttgatgccgg
    10081 caagtgtttc ggcgggagac ttgttgttgg tggccttgaa gatgccgccg agcagaatcg
    10141 gcacatattc gaatttcacg ccggtgcgtt gttcgattcc cgggatcgcc tcgtgactga
    10201 gaaaggcatt cgggctgccg acatcgaaca agaattgcgg cgacgtctgg gtcaaggcgg
    10261 cctcccgggc tttttctgtt gccgccgcat tttggcgcag cgcggccggc gcgtccactg
    10321 catttatgac gaccataata ctctggaacc gcgaaggacg gtcagatcag ccgtcgtatc
    10381 gtgcgcttgc gccataccag catgtagtag ctcatctgca gaatgaacat ggcgg
//
LOCUS       DATNOI010000594        26750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_85243,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000594 DATNOI010000000
VERSION     DATNOI010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 26750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26750
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85243"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VN968_24050"
     CDS             <1..265
                     /locus_tag="VN968_24050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32338.1"
                     /translation="AKVRPGSGLFEMWDIEERNNDRYVLDHGASGAPIVDCEGNVAAV
                     ASSIVTQGSLYFAGREFTLTTPWGMANNTAALIQPLFDIALPQ"
     gene            complement(348..2063)
                     /locus_tag="VN968_24055"
     CDS             complement(348..2063)
                     /locus_tag="VN968_24055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HXL32339.1"
                     /translation="MKISRQIGSLVVSGIALAALSLPAIAQAPAGKPNILFIMGDDIG
                     WMQPSIYHRGLMVGETPNIDRIGNDGAIFMDYVAMQSCTSGRNAFFTGMYPLRTGMIP
                     PQLPGSPSYLRPGTPALARFLLDLGYNTGEFGKNHLGDHTASLPTAHGFQEYWGYLYH
                     LDAMQQVSFPDINSSPTVQGVAPPCRNTPIPGIAEVPGAVDQKTTLCLMPPRPVLACK
                     SADGTEKNQMCKDEGPLTLERSKTVDEEISAKVIDFLDRNDPKKTNKPFFAWYNPARM
                     HVTTVLSPKYMAMVGEQGGKDWGINEAGMKQMDDNIGLVLKKLEDIGQLDNTIVVFTT
                     DNGAEAISYPDGGVTPFKGQKGEAWEGGYRAPMVIRWPGHIRPGTVKNKLFAALDWMP
                     TLVDIAGGPKGDGLKTQIEAGQYPGIVKTTLDGFDQRAYLEGTSEKSARDYFFYFSGA
                     TPSAVRYKNWKMYYTMSQPGPAGWILPLIPFHFTLVNNIKRDPFEQAVGIDQKTAMSL
                     GGALGGPVTAFQYDWNMLPIGQQLWMEHLLSYEKFPPLQAPESYNLSGILDHVRKANA
                     SHASD"
     gene            complement(2187..2654)
                     /locus_tag="VN968_24060"
     CDS             complement(2187..2654)
                     /locus_tag="VN968_24060"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL32340.1"
                     /translation="MLHALVNCLTADDANGNPKTRRHHADVMVRFEEALTAHIGRQLN
                     MPALCSAIGVPERTLRVCCSEFLGISPTRYLLLRRLNLARSALRRADPATASVAEIAR
                     TYQFREPGRFAVTYRTIFGEMPSSTLRRKSIKNINLPKSHSVCNTTCGKSPDR"
     gene            complement(2853..3200)
                     /locus_tag="VN968_24065"
     CDS             complement(2853..3200)
                     /locus_tag="VN968_24065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32341.1"
                     /translation="MAENGTATFADPDDYKAGIDGARVNLIVTGGGDFNARLTWLNLR
                     RLRVLRASENPPRIAFFSLSPAQVFVSFPASTGPPLTYGGVGLQFGDVVFHSRGERMH
                     QRTDGESQWGLIS"
     gene            complement(3987..4985)
                     /locus_tag="VN968_24070"
     CDS             complement(3987..4985)
                     /locus_tag="VN968_24070"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL32342.1"
                     /translation="MPWSKVLNFTDPLPYQAAIRAADLELFPTARGKFCAELTQVCMN
                     QLWMQRFHQNLPQVVAGTMRAGRRIISFLAAAKPTAMQHRGMDVSPGEIIVNNSDVLH
                     QRTGAGFRMGGISLPTQDFDVAYKAITGREFTGSPLKRLVRPDTALMSRLLNLHEMVG
                     QLAKTTPDLLALPEVARALEQELIHLMVRCLIDSVSSGMTAGGRRHDAIVARLEEFLE
                     SHPDQPLYLTEICAAIGVAERTLRAACKEHLGMGPIRYLGLRRMHLVRRALLRADPSK
                     TVTHVATDHGFWELGRFAVAYRALFGESPSATLHRPSDDMQIFLNRPSSLATSRFA"
     gene            complement(5361..7679)
                     /locus_tag="VN968_24075"
     CDS             complement(5361..7679)
                     /locus_tag="VN968_24075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXL32343.1"
                     /translation="MTPFVPPPTLRRTALGTRALVTLALAVGLTGLAADAWAKPKVPT
                     PRPRPIARNVVPKTLDAKTLDAKTLDARMPAATASAPTSPAPKTAAKNTAPAPNPATA
                     APAAPAMAPATRQHAALPPAPARRPVAPAAVAATTSTSQSDTEALENVIDLVRKHKPS
                     EATQAEAVISDPVARKLAEWIILRSDDNGASVERYRTFIAANPSWPSQSFLRRRLEAA
                     LWDDHRDDATVWAWFENESPLSAKGRFSLARVMIARGDRANAERLVREAWRNDSMSED
                     TESTALDLFGALLTPGDHKARMDTMLYGSEQEAGLRAAKRLGAAHVALAKARNAAYRK
                     APNTKALLDAVPQELHGDPGYVFSRIQLLRRDEKFAEAAQLMLSAPKDPGRLYNLDEW
                     WIERRLLSRKMLDIGEHRTAYLIARDAALPARDIYKTEQEFTAGWIALRFLKDPATAA
                     QHFARIGVGSANPTALARGGYWQGRAAEAAGRSQDARSAYTAAAEQSTSYYGQLARAK
                     LGLPKIELNGAPAARGRAIERLEIVRAVQLLYGLDEREIAIPIFADMGENGDPDALAG
                     LGELASRNADARSMLLLGKAALNRGLPFDFYAYPVTGIPPFKSIGPDVEQSIVFAIAR
                     QESAFNPAVVSPAQAYGLMQVTPDAGKYVCKKYGASFDLGRLKTDPVYNAALGAAELG
                     GLIEDYRGSYIMTFAAYNAGRGSVKKWIERYGDPRDPKVDAVDWVELIPFSETRNYVQ
                     RIMENLQVYRARFGGGTRLQIEADLHRGASVE"
     gene            8036..8926
                     /gene="dapA"
                     /locus_tag="VN968_24080"
     CDS             8036..8926
                     /gene="dapA"
                     /locus_tag="VN968_24080"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HXL32344.1"
                     /translation="MAAKTNFRGSFTALVTPFKNGSLDEAAFRALVNWQIAEGTHGLV
                     PVGTTGESPTVNHDEHKRVVEWCIDEAGGRVPVIAGAGSNSTREAIELSEHAERAGAS
                     AVLVVTPYYNKPTQEGMYQHFKAINDAIGIPIIIYNIPPRSVVDMSVETMARLFELKN
                     IAGVKDATASMVRVSQQRATLGEDFNQLSGEDATVLGYMAHGGHGCISVTSNVAPRLC
                     SEFHLAWQKGDLATALKLHDKLMPLHTNLFIESNPAPVKYALSLLGKIDEKLRLPMVP
                     VTEPTRLAVRSAMVHAGLIN"
     gene            9089..9502
                     /gene="mscL"
                     /locus_tag="VN968_24085"
     CDS             9089..9502
                     /gene="mscL"
                     /locus_tag="VN968_24085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027547170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXL32345.1"
                     /translation="MLKEFREFAMKGNVVDLAVGVIIGAAFGGIVTSLVGDIIMPIIG
                     AITGGLDFSNYFTGLSKAVTASNLADAKKQGAVLAWGNFLTLTLNFIIIAFVLFAVIR
                     FINTLKRKDEAAPAQPKPTRQEELLTEIRDLLKKS"
     gene            9569..10042
                     /gene="smpB"
                     /locus_tag="VN968_24090"
     CDS             9569..10042
                     /gene="smpB"
                     /locus_tag="VN968_24090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HXL32346.1"
                     /translation="MAEKNERAIKVVAENRKARFNYAIEDTVEAGIALTGTEVKSIRN
                     GKTTIAESYADTKGGEIWLINANIPEYLQANRFNHEPKRPRKLLLHRKQINKLMGAVD
                     RDGMTLIPLKLYFNERGRAKLLLAVAKGKKLHDKRESEKKRDWGREKGRLLRARG"
     gene            10230..10805
                     /locus_tag="VN968_24095"
     CDS             10230..10805
                     /locus_tag="VN968_24095"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXL32347.1"
                     /translation="MNQKNLIEVDWSKIPAPADDGAAAHLKGMTVPPISLIATDDSSV
                     TLSALPGRVVVFAYPRTGEPGKIALVDDWDIIPGARGCTPQTCAFRDLHAELKASGTR
                     YVFGLSTQSNAYQTEMASRLHLPFPVLSDEKLTLTHALDLPTMEVAGLTMIKRLALIV
                     DDGRITQVFYPVFPPDRNAGDVLAWLKENPV"
     gene            complement(10984..11679)
                     /locus_tag="VN968_24100"
     CDS             complement(10984..11679)
                     /locus_tag="VN968_24100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXL32348.1"
                     /translation="MTTNSTIEKGPARGGPLLAAALLRSEPDRNCPLCPRLSGFREAT
                     RAREPHWFNSPVPSFGDPDARLLIVGLAPGLQGANRTGRPFTGDYAGDLLYATLLEYG
                     FAKGHYQARPDDGLTLVDCRISNAVRCVPPQNKPQPAEINVCRQFLIATIATMPNLRA
                     IVALGRVAHESTLKALGLRAAAAPFAHGAVHQAGVTKFYDSYHCSRYNTNTRVLTPQM
                     FRSIFAKVRAELD"
     gene            complement(11657..12286)
                     /locus_tag="VN968_24105"
     CDS             complement(11657..12286)
                     /locus_tag="VN968_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXL32349.1"
                     /translation="MSPASNKIALFIDGANLYATAKTLGFDIDYKRLLKEFQSRGTLL
                     RAFYYTAIIEDQEYSSIRPLIDWLDYNGYTVVTKATKEFIDASGRRKVKGNMDIELAV
                     DAMELAEHVDQIVLFSGDGDFRSLVEAVQRRGVRVTVVSTISSQPPMIADELRRQADV
                     FTDLVELQSKLGRDPSERPPPREPRHHAPQFLQRATTMAPRGDDDEFDD"
     gene            12629..13021
                     /gene="rpoZ"
                     /locus_tag="VN968_24110"
     CDS             12629..13021
                     /gene="rpoZ"
                     /locus_tag="VN968_24110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HXL32350.1"
                     /translation="MARVTVEDCIDKVDNRFDLVLLAAHRARMISSGSQLTVDRDNDK
                     NPVVSLREIADSTISPEDLREELVHSLQKFVEVDEPEPDTVPLIGSAGASVDADDTEV
                     AVERMTEEELLKGLEGLAPPEEQPEEDE"
     gene            13304..15601
                     /locus_tag="VN968_24115"
     CDS             13304..15601
                     /locus_tag="VN968_24115"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HXL32351.1"
                     /translation="MAFRRHSPWQMQAATDSVAVAPSSAAPAKPARSPRPRMMRQYDL
                     VERVRSYNPDTNEDQLNRAYVYAMKAHGTQTRASGDPYFSHPLEVAAILTDLKLDDAT
                     IVAALLHDTIEDTEATRAEIDHIFGHEIGALVEGLTKLKRLELVSREAKQAENLRKLL
                     LAIADDVRVLLIKLADRLHNMRTLEFVPPTARRRIAEETLDIYAPLAGRMGMQEMREE
                     LEDLSFRTLDPEAYAVVMQRLDALAERNRNLIGEIESQLTKNLQNNGVPAHVYGRRKQ
                     PFSIWTKMERKSVGFEQLSDIFGFRVVMDDVEACYRALGVVHTTWPVVPGRFKDYIST
                     PKQNDYRSIHTTVIGPGNQRVELQIRTEEMDEIAELGIAAHAFYKDGVGSPTDLLKRE
                     SNAFAWLRHTVGILSESANPEEFLEHTKLELFHDQVFCFTPKGKLIALPRQANVIDFA
                     YAVHTDVGNSAVGCKINGKFAPLSSELQNGDEVEVLTSEAQSAPPSAWESLAVTGKAR
                     AAIRRATRTAVRDQYAGLGRRIVERLFVRAKIEYADDKLKGALPRLARASIDDVMAAV
                     GRGEMKASDVARAMYPDYKEERVARYGAPKKSLAVKLKLKSPPDPARSPSAIPVRGIN
                     SDLPVKFAPNGGAVPGDRIIGIVSPGEGITIYPIQSPALKDFEDEPERWLDVRWDVDE
                     TTPQRFPARIMVDNVNEPGSLAQVATVIAEHDGNIDNISMSRRSPDFTELTIDLEVYD
                     LKHLSAIIAQLRAKAVVARVERVNG"
     gene            15766..16563
                     /locus_tag="VN968_24120"
     CDS             15766..16563
                     /locus_tag="VN968_24120"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HXL32352.1"
                     /translation="MPKPPPLRLGVNVDHVATLRNARAGRNPDPVRAALVAIAAGADG
                     ITAHLREDRRHIRDEDMARLKAEISKPLNFEMAATEDMLRIALATRPHAVCLVPERRE
                     ELTTEGGLDVVAQHNALAPFIARLNDAGVRVSLFIGAEPRQIEMAAKLRAPVIEIHTG
                     AWCDAVVDGHDARAESEWQRIVAGTRLARSAGLEVHAGHGLDYVTAEKIAGLPEIVEL
                     NIGYYMIGEALFTGLGETVRAMRAAMDRGRNMAAGQSPDGEKPAGKT"
     gene            16560..16979
                     /gene="acpS"
                     /locus_tag="VN968_24125"
     CDS             16560..16979
                     /gene="acpS"
                     /locus_tag="VN968_24125"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HXL32353.1"
                     /translation="MILGIGSDLIDIRRIAKVIERHGDRFLDRIFTEAERARAARRAK
                     SEKMVVATYAKRFAAKEACSKALGTGIRRGVWWRDMGVVNLPGGRPTMQLTGGALARL
                     EAMTPPGHEARVDLSITDDWPLAQAFVIISAVNVGKP"
     gene            17420..18178
                     /gene="lepB"
                     /locus_tag="VN968_24130"
     CDS             17420..18178
                     /gene="lepB"
                     /locus_tag="VN968_24130"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXL32354.1"
                     /translation="MSVTSGTKSESGFGETVRVVIHALLIALVIRTFLFQPFNIPSGS
                     MKATLLVGDYLFVSKYSYGYSHYSIPLSPPLFSGRIFGSEPARGDIVVFRLPKDDTTD
                     YIKRVIGLPGDRIQMKDGLLYINEKPVTRERLSDFVGEDPCGSDATSRVKRWKETLPN
                     GVTYQSLDCVDNGFYDNTSVYTVPPGHFFMMGDNRDNSTDSRVLSAVGYVPFENIVGR
                     AQMIFFSIAEGEHAWMLWRWPTAVRWNRIFSIVR"
     gene            18175..18990
                     /gene="rnc"
                     /locus_tag="VN968_24135"
     CDS             18175..18990
                     /gene="rnc"
                     /locus_tag="VN968_24135"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HXL32355.1"
                     /translation="MNDETADIRILSSSGDAGHQLERASEPAAKKKRSKISAKAATAA
                     IEARIGHKFADATLLATAFTHVSALKSARKRGDSYQRMEFLGDHVLGLIVSDMLYRAF
                     PNADEGELSKRLADLVRKESCADVAKSLGLVDDIKLGAVGAGAGARLRKSVLGDICEA
                     VIGAVFLDGGYSAAAQFVERNWTERMRKPRRPLRDPKTVLQEWAQGKGLPTPVYREIE
                     RTGPHHDPQFRVAVDLPGLAPAEGVGGSKRAAEKVAASVMIAREGVGGGSNDG"
     gene            18983..19909
                     /gene="era"
                     /locus_tag="VN968_24140"
     CDS             18983..19909
                     /gene="era"
                     /locus_tag="VN968_24140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HXL32356.1"
                     /translation="MDELPDDAAPVATRCGFVALIGAPNVGKSTLVNALVGSKVTIVS
                     RKVQTTRALIRGIVIDNNAQIILVDTPGIFLPKRRLDRAMVSTAWSGARDADLVCVLL
                     DAREGIDEEADAILTKVATVRHERILVLNKIDLVPREKLLALTKAANDRLSFEKTFMV
                     SALSGDGVADLRHTLAAMVPPGPFHYPEDQMSDAPMRHLAAEITREKIFRQLHQELPY
                     QSTVETDSWTERKDKSIRIEQTIFVERESQRKIVLGKGGATIKSIGADSRKEIAEIVG
                     VPVHLFLFVKVRENWGDDPNRYREMGLEFPKE"
     gene            19922..20302
                     /locus_tag="VN968_24145"
     CDS             19922..20302
                     /locus_tag="VN968_24145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32357.1"
                     /translation="MNVPKNVVWFEVLLYMSLTLDALSVAFQDRTPSAEMTEQMITVA
                     TVMAAFLILLLVFFVWLAAQRRKNWPRWALAAAGVLSIIQLVQIIADSGLQLDSGIEI
                     VSCILTAAGLYFSFTGDAQGWFNG"
     gene            20405..21154
                     /gene="recO"
                     /locus_tag="VN968_24150"
     CDS             20405..21154
                     /gene="recO"
                     /locus_tag="VN968_24150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HXL32358.1"
                     /translation="MEWTDEGIVLGVRRHGESSAIVELLTRGHGRHLGLVRGGAGSRM
                     RPLLQPGNSVRAVWRARLDEHLGYYQIEGTRLRAATVLASSHAVYGVTHLASLARLLP
                     ERDPHEDIYEMLERTLDDFDDIGEAAVHLVKFELAMLAELGFGLDLETCAATGGTSEL
                     IYVSPKSGGAVSRQAGDPWRDRLLRLPPFLRSSGGGPNGWSDQDLQDGFALTGMFLLR
                     HVLEPRGQGHSDARDGFINAVTKHRARMALP"
     gene            21378..21635
                     /locus_tag="VN968_24155"
     CDS             21378..21635
                     /locus_tag="VN968_24155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32359.1"
                     /translation="MWIRWTCVAAICLGLTSQAEAAEHSGLVHRASCAVVRFYVAKFS
                     AFAAETWARSHGATEAEIDAARHCLKDAPAQTAQASQYSKF"
     gene            22150..22317
                     /locus_tag="VN968_24160"
     CDS             22150..22317
                     /locus_tag="VN968_24160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32360.1"
                     /translation="MMIDPKSRLIAALGNAQEYIDLAASAKDRGEREFYERIAELYIR
                     IAQELDALVEG"
     gene            22492..22695
                     /locus_tag="VN968_24165"
     CDS             22492..22695
                     /locus_tag="VN968_24165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32361.1"
                     /translation="MYDRTDLVTIATAVCLAGYAAIALWYVTLFDIGLTREGVRSGAT
                     VCAILLAAVVAMDFLARKLGKRE"
     gene            22837..25092
                     /gene="parC"
                     /locus_tag="VN968_24170"
     CDS             22837..25092
                     /gene="parC"
                     /locus_tag="VN968_24170"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HXL32362.1"
                     /translation="MGKRLIPPEPAEIHEVMLRDALEERYLQYALSTIMHRALPDARD
                     GLKPVHRRILYGMRLLRLDPGTPFKKSAKIVGDVMGSFHPHGDQSIYDALVRLAQDFA
                     SRYPLVDGQGNFGNIDGDNPAAYRYTEARMTEVARLLLEGIDEDGVEFRPNYDGQSKE
                     PIVLPGGFPNLLANGSQGIAVGMATSIPPHNAAELCDAALHLIDKPEAKSKALLKWVK
                     GPDFPTGGIIVDSKESIAEAYMTGRGSFRTRARWSQEEGARGTWVIVITEIPWLVQKS
                     RLVEKIAELLNDKKLPLIGDVRDESAEDIRLVIEPKSRAVDPELLMESLFRLTELESR
                     ISLNLNVLVKGRIPKVLGLAECLREWLDHLRDVLLRRSNYRKVQIEHRLEVLGGYLIA
                     YLNIDKVIKIIRTEDEPKPALIKAFKLTEIQADAILNMRLRNLRKLEEFEIRTEDKNL
                     RAELKGIKELLGSETAQWSKVGEQVRKVRDLFGPKTPLGKRRTTFADAPEHDLAAIEE
                     AFVEREPVTVVVSDKGWVRTLKGHVADLSGLTFKTDDQLGSAFFAETTSKLLLFATNG
                     RFYSLEVSKLPGGRGHGEPIRMFIDMEQDASIVSLFVNKGGRKFLVASHEGQGFVVNE
                     DDCVGNTRKGKQVLNVEMPNEARALTTVTGDTVAVIGDNRKMVLFPIDQVPEMARGRG
                     VRLQKYKDGGLSDIATFDAKDGLSWKDSAGREFSATIKELADWRGNRADAGRLPPKGF
                     PKSNKFGRGIE"
     gene            25252..26055
                     /locus_tag="VN968_24175"
                     /pseudo
     CDS             25252..26055
                     /locus_tag="VN968_24175"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(26146..>26750)
                     /locus_tag="VN968_24180"
     CDS             complement(26146..>26750)
                     /locus_tag="VN968_24180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HXL32363.1"
                     /translation="AVDPDVRGAFQSALASLASLGAEILPDCVAFAPDMLDRILKPIA
                     YTEQAAAVAGLGADALALSDWDYREVIARGRGYLGVDYVEAGYRRNSLRASFQELFQT
                     VDALVTPTVAITAFPAGTLGVDRVAGVAVDRHLGWSPFSWPINLTGLPAATIPCGFDT
                     DGLPIGLQIVAPWLDEGVILRIAAAFEWAHPWAQFRPRMT"
BASE COUNT         5200 a         8087 c         8444 g         5019 t
ORIGIN      
        1 tgccaaagtg cgaccgggat cgggtctctt cgagatgtgg gatatcgagg agcgcaataa
       61 cgaccgttat gtgctcgacc atggcgcgtc aggggctccc atcgtcgact gcgagggcaa
      121 tgtggcagcc gtagccagta gcatcgtgac ccaagggagc ttgtacttcg ccggacggga
      181 attcacgctg accaccccat ggggcatggc aaacaacacc gcagcactta tccaaccgct
      241 gtttgatatt gcacttcctc agtaaccacc tggcgaaaag ctggcgcggt tcgcgggcgc
      301 cccatcgtgg ggcacccgct ccgtaacatc acgctcgtct cagacggcta gtcgctcgcg
      361 tgactggcat tagccttcct gacgtgatcc aagatcccac tgaggttgta ggattcggga
      421 gcctgcagtg gcgggaactt ctcgtacgac agcaggtgct ccatccacag ctgctggccg
      481 atcggcagca tattccagtc gtattggaac gcggtcaccg ggccgccgag cgcaccgccc
      541 aggctcatgg ccgtcttctg gtcgatgccg accgcctgct cgaacggatc gcgcttgatg
      601 ttattgacca aggtgaagtg gaaggggatg agcggcaaaa tccacccggc cggcccgggc
      661 tgcgacatgg tgtagtacat cttccagttc ttgtagcgca ccgccgacgg cgttgccccc
      721 gagaaataga agaagtagtc gcgcgccgac ttttccgacg tgccttcgag ataggcgcgc
      781 tggtcgaagc catcgagcgt cgtcttgacg atgccgggat actgtccggc ctcgatctgc
      841 gtcttcagcc cgtcgccctt gggcccgccc gcaatgtcga ccagcgtggg catccagtcc
      901 agcgctgcga acagcttgtt cttgaccgtg cccggcctga tgtggcccgg ccagcggatc
      961 accatcggag cgcgatagcc gccttcccag gcctcgccct tctggccctt gaacggggtg
     1021 acgccgccgt cggggtacga gatagcctcg gcgccgttgt cggtggtgaa cacgacgatg
     1081 gtattgtcga gctggccgat gtcttcgagc ttcttgagca cgaggccgat gttgtcgtcc
     1141 atctgcttca tgccggcttc gttgatgccc cagtccttgc cgccctgctc acccaccatg
     1201 gccatgtact tgggcgacag cactgtggtg acgtgcatgc gcgccgggtt gtaccaggcg
     1261 aagaaaggct tgttggtctt cttggggtcg ttgcggtcga ggaagtcgat gaccttggcg
     1321 gagatctcct cgtccaccgt cttcgaacgc tccagcgtca gggggccctc gtccttgcac
     1381 atctggttct tctccgtgcc gtcggcggac ttgcacgcga gcaccgggcg cgggggcatc
     1441 aggcagagcg tcgttttctg gtccaccgcg ccgggaacct ccgcgatgcc ggggatcggc
     1501 gtgttgcggc acggcggggc gacgccctgc acggtcggac tgctgttgat gtccgggaag
     1561 ctcacctgct gcatcgcgtc gaggtgatag aggtagcccc agtattcctg gaagccgtgg
     1621 gccgtcggca gcgatgcggt gtgatcgccg aggtggttct tgccgaactc gccggtgttg
     1681 tagccgagat ctaacaggaa cctggcgagc gccggggtgc cgggccgcag ataggacggg
     1741 ctgccgggca gctgcggagg gatcatgccg gtgcgcagcg gatacatgcc ggtgaagaag
     1801 gcgttgcgcc cggaggtgca gctctgcatg gcgacataat ccatgaagat cgcgccgtcg
     1861 tttccgatgc ggtcgatgtt cggcgtttcg cccaccatca agccgcggtg gtagatgctc
     1921 ggctgcatcc atccgatatc atcacccatg atgaacagga tgttgggctt gccggccggt
     1981 gcttgcgcga ttgcaggcaa gcttaacgcc gccaaggcaa tacctgaaac taccaggctc
     2041 cctatctgtc gactgatctt catgttcgag atcctccgtt gctacttttg atcggctatt
     2101 aggcactgtt ttcggattgc ccatgcagaa tgcggcaaca tgtcgtcgct cttcgcctcc
     2161 ggccaccgcc caggctgtcc gcttcattat cgatccggcg attttccaca ggttgtattg
     2221 cacacgctat gcgatttcgg cagattgata tttttgatcg attttcttcg aagtgtcgac
     2281 gagggcattt ccccgaagat ggtgcggtag gtcacagcga agcgccccgg ctcccggaat
     2341 tgataggtcc gggcaatctc tgcaacactc gcggtggccg ggtcggcccg tcgcaacgcc
     2401 gagcgcgcca agttcagtcg tcgcagcaga agataacggg tgggactgat gcccaggaat
     2461 tcgctgcagc acactcgcaa cgttcgttcc ggcacgccga tcgccgagca aagcgcgggc
     2521 atgttcagct gccgtccaat atgcgcggtc aacgcttcct cgaaccgaac catgacatcg
     2581 gcgtggtgcc gcctcgtttt cggattgccg ttagcatcat cggccgtcag gcaattgacc
     2641 agcgcgtgaa gcaattgctg ctcgagtgct cgcgcgactt ccggattcgc aattaattta
     2701 ggtctggttt cggcgaggcg gcaagccttt gaatgaagcc gcagcaggcg tgaggctgct
     2761 acccgcgacg gtcgcagtac ccggctgaca ggcggcgcaa ttatcttcag tccagtcagg
     2821 gccttgccgc aggcggcgag ttgctcgggc ggctacgata taagacccca ttggctttct
     2881 ccatcggtcc gctgatgcat tcgctcaccg cggctgtgaa acacgacatc gccgaactgc
     2941 aatccaactc cgccatatgt taagggcgga ccggtgctcg ctggaaacga aacaaacacc
     3001 tgcgccggcg acaacgagaa aaaggcaatg cgcggcggat tttcggaagc acgaagcacg
     3061 cggagacggc gcaagttcaa ccacgtcagg cgcgcattga aatctccgcc gccggtgacg
     3121 atgagattga ccctcgcgcc gtcaataccg gctttgtagt cgtccgggtc ggcaaacgtg
     3181 gccgtgccgt tttccgccat ccgctcatct cattcgacac gcggcgccat tgcgaaccgc
     3241 aatcgctaaa acgcaggatg ggggaccggc tccgatttcg ctatgcgact tcggaagctg
     3301 acaaattatt taagttcaat acgttacgcc gcgttgaaga ttgaatatct ctgataattt
     3361 aaggagcccc gtcgtcgggg cgaccgagct gcgcgaaata agagaccgcc cgaaggtggt
     3421 ctctaatcgc ggtttgggtc ggcgtcagta gcaaggttat cagcggctca cgctaatccc
     3481 gcgcagcgcc ctccattatt cttaaaggag ggatagactg ctcaacgtta gggtctaatg
     3541 cctgctttga agcagcactg gcgataggca tcctgtccga cggcgttctt atcggcggta
     3601 ttagcgctaa agcccgtggc ggccacagct tcattggctt gcctaaaaca ttcagcacgt
     3661 gccgcactgg gggttggact tcgccgactt cgccgcctct gctgggattg cagcgcctag
     3721 aagtgcaaca agaacagcaa aggcgaatag agatcttgaa ctcaacatgc tggcctcctt
     3781 tttcaaggga taaaggatga ccaagcaaag tcgtttccga actatgtcga tgccggtcac
     3841 ccttccggta ttcgggccgg ttcgattgtt gcctctcgaa catgtccaaa gtctaatgct
     3901 tagggatcaa tgctcgcgcg agagccgatc ttaggatcag cctaactttc gtctatgcaa
     3961 aaacggcaga ttggtcggtt gcgtaactaa gcaaaccggg atgtcgcgag cgacgacggg
     4021 cgattgagaa atatttgcat gtcgtctgac ggtcggtgca atgtcgcaga aggtgactcc
     4081 ccgaacagtg ctcgataagc gactgcgaaa cgacctaact cccagaagcc atgatcggtg
     4141 gcgacatgcg ttacggtttt cgaaggatcg gctcgtaaaa gcgcacgtct aaccagatgc
     4201 attcgccgca agcccaggta tcggatcggc cccattccca aatgctcctt gcaggcggca
     4261 cggagcgttc gctccgctac gccgatggca gcgcaaattt ccgtcaggta cagcggctgg
     4321 tcggggtgcg attccaggaa ttcttcgagc cgcgcgacga tcgcatcgtg gcgacgaccg
     4381 ccggcggtca ttcccgacga cacgctgtcg atcaggcacc tgaccatcag gtggatcagt
     4441 tcctgctcga gggcccgagc tacctcggga agcgcgagaa gatccggcgt ggttttggcg
     4501 agctggccaa ccatctcatg aaggtttagc agccgcgaca tgagggcggt atccggccga
     4561 acgagacgct tcagcggtga tcccgtaaat tcgcgaccgg ttatcgcctt gtatgcgaca
     4621 tcaaagtctt gtgtcggcag ggaaatccct cccatacgga agccggctcc agttcgttgg
     4681 tgcaacacgt cggagttgtt gacaatgatc tcgccgggcg aaacatccat gccgcggtgc
     4741 tgcatcgctg tcggttttgc cgcggcgagg aagctaatta ttctgcgacc agccctcatt
     4801 gtcccagcga caacctgcgg cagattttga tggaagcgct gcatccaaag ctggttcata
     4861 cagacttgcg tcaattcggc gcaaaatttc cctctcgcgg tgggaaaaag ctccaaatct
     4921 gcagctcgaa tggccgcctg ataaggaagt ggatcggtaa agttgagaac tttgctccaa
     4981 ggcatttcca tactcccgga gttaccgaag ctgaaagttg cccgccccca aggccaatgc
     5041 aatgagtgac tattttctta ccgacgatcg ccaggaatta tttggcaggt tgacataggg
     5101 gctgcgcgaa aaaatgatct gctacgacct cgccattaac gtgctacgtc acagcagcac
     5161 gctatctagt tgctgtgtcc ggaagattac ataaaagcac gagatgcaca aagtaaaaga
     5221 ccgccttccg gcggtctctg gctaccagca tcttcagttc atagggcggc gccttgcaac
     5281 gatagaagct gccgcgcgag gcccatttgc aaaatagtgg tgcgagcggg cacggcggca
     5341 acggaaaatc ttgaagaaat ctattcgacg cttgcgccgc ggtgcaggtc ggcctcgatc
     5401 tgcagccgtg ttccgccgcc gaatcgcgcg cgatagacct gcagattctc catgattcgc
     5461 tgcacgtagt tgcgggtctc ggagaacggg atcaattcga cccaatcgac cgcatcgacc
     5521 ttgggatcgc gcggatcgcc gtaacgctcg atccattttt tgacgctgcc gcggccggca
     5581 ttgtaggccg cgaatgtcat gatgtaagag ccgcgataat cctcgatcag cccgccgagt
     5641 tccgcggcgc caagggccgc gttatagacc ggatcggtct tcagccggcc gaggtcaaag
     5701 ctggcgccgt atttcttgca tacatatttg ccggcatccg gcgtcacctg catcagcccg
     5761 tacgcttgcg ccggtgaaac caccgccgga ttgaaggcgc tctcctgtcg tgcgatggcg
     5821 aacacgatgc tctgctcgac atccggcccg atcgacttga acggcggtat ccccgtcact
     5881 ggataggcgt agaaatcgaa tggcagcccg cgattgagcg cggccttgcc gagcagcagc
     5941 atgctgcgcg cgtcggcatt gcgggaggcc agttcgccga gccccgccag cgcgtcggga
     6001 tcgccgtttt cgcccatgtc ggcgaaaatc gggatcgcga tttcacgttc atcgaggccg
     6061 tagagcagct gcaccgcacg gacgatctcc agccgttcga tcgcacgtcc gcgcgccgcg
     6121 ggcgcgccgt tcagttcgat cttcggcaat ccgagtttgg cacgcgccaa ctggccataa
     6181 taactggtcg attgttctgc cgccgccgtg taggcggagc gcgcgtcctg cgagcggccc
     6241 gccgcctcgg cggcacggcc ctgccaatag ccgccacgcg cgagcgcggt cggattggcg
     6301 ctgccgacgc cgattcgcgc aaaatgttgc gcagctgtcg ccgggtcctt cagaaagcgt
     6361 agcgcgatcc agcccgcggt gaattcctgc tcggtcttgt agatgtcgcg cgccggcagc
     6421 gcggcgtcgc gggcgatcag ataggcggtg cggtgctcgc cgatatcgag cattttgcgc
     6481 gacagcaatc gccgctcgat ccaccattcg tcgagattgt acagccggcc cgggtccttc
     6541 ggtgcgctca gcatcagctg cgcggcttcg gcgaatttct catcgcggcg gagcagctga
     6601 attctgctga agacgtagcc gggatcgcca tgcagttcct gcggaaccgc gtcgagcagc
     6661 gctttggtgt tgggtgcctt gcggtaggcc gcgttccggg ctttcgccag cgcaacgtgt
     6721 gccgcgccca atcgcttggc ggcgcgcagg ccggcttcct gctcgctgcc gtagagcatc
     6781 gtatccatcc gcgccttgtg gtcgcccggc gtcagcaagg cgccgaacag atcgagcgcg
     6841 gtgctctcgg tatcttcgga catgctatcg tttcgccagg cctcgcgcac cagccgctcc
     6901 gcgttggccc ggtcgccgcg cgcgatcatg acccgcgcca gcgagaatcg gcctttggcc
     6961 gacagcggcg attcgttttc gaaccaggcc cacacggtgg cgtcgtcgcg atggtcgtcc
     7021 caaagggcgg cttcgaggcg ccggcgcagg aagctctgcg agggccaact cggattggcc
     7081 gcgatgaatg tgcggtaacg ctcgaccgaa gcgccgttgt cgtcgctgcg caggatgatc
     7141 cattcggcga gcttcctcgc gaccgggtcc gatatcaccg cttcggcttg cgtcgcctcc
     7201 gagggcttgt gcttgcgcac gagatcgatg acgttttcga gcgcctcggt atcggactgc
     7261 gaagtcgatg tggtcgcggc aaccgccgcc ggggctaccg gtctgcgcgc cggtgcaggc
     7321 ggcaaggccg catgctggcg tgtcgcgggc gccatggcag gcgctgccgg agccgcggtc
     7381 gcggggtttg gcgcaggcgc ggtattttta gccgcggtct tgggagcggg actcgtggga
     7441 gcagaagccg tggcagcagg catcctggca tcaagagtct tggcatcaag agtcttggca
     7501 tcaagagtct tgggcacgac gttgcgcgcg atcggccgcg gcctgggcgt gggaactttt
     7561 ggcttggccc aggcgtccgc cgccaatccc gtcaatccca ccgccaacgc cagcgtcacc
     7621 agcgcgcggg tgcccaaagc ggtccgtcgc aatgtgggtg gggggacgaa tggcgtcacg
     7681 gcgttccttc gcggcgaatc aatcaagcgc tgaagcccta gctctactta attgaatata
     7741 tggacaaaat actaaatacg cggggattgg cgatgtctgc gccattaccg cggcaaaatc
     7801 ggggctacgg cgttttcaag cgaaagtctg ccccggacgc gatccggggt ggagaccgtt
     7861 cgcgtcaaga aaacgcgtca gaccaggaat ctggagcccc gtttcgattc gatcgaaacg
     7921 aaaaaggctc gagggtcgga ccggcgcggg cctttcgccc gatcctcaga cccgttatga
     7981 attggccctt atcgccggtt cagacggctg gtggcgtgcg tttaggagaa agtccatggc
     8041 agccaagacg aatttccggg gctcgttcac cgctttggtc acgccgttca agaacggctc
     8101 gctcgacgag gcggcctttc gcgccttggt gaactggcaa atcgccgagg gcacccacgg
     8161 cctggtgccg gtcgggacga ccggcgaaag ccccaccgtg aaccacgacg agcacaagcg
     8221 tgtcgtcgaa tggtgcatcg acgaggccgg gggccgggta ccggtgatcg cgggcgccgg
     8281 ttccaactcg accagggaag ccatcgaact cagcgagcac gccgagcgcg ccggcgccag
     8341 cgcggtactg gtggtgacgc catactacaa caagccgacc caggaaggca tgtaccagca
     8401 cttcaaggcg atcaacgacg cgatcggaat tccgatcatc atctacaaca ttccgccgcg
     8461 ctcggtggtc gacatgtcgg ttgagaccat ggcgcggttg ttcgagctga agaacatcgc
     8521 cggcgtcaag gatgcgaccg cgagcatggt gcgggtgtcg cagcagcgcg caacgctggg
     8581 cgaggatttc aaccagttgt cgggcgagga cgccaccgtg ctcggttaca tggcccatgg
     8641 cggtcacggc tgcatttccg tcacgtccaa tgtcgcgccg cggctgtgct ccgaatttca
     8701 cctggcctgg cagaagggcg atctcgccac cgctctcaag ctgcacgaca aactgatgcc
     8761 gctgcacacc aatctcttca tcgagtccaa tccggcgccg gtgaaatacg ccctgtcgct
     8821 gctcggcaag atcgatgaga agctgcgcct gccgatggtg ccggtcaccg agccgacgcg
     8881 gctggcggtg cgcagcgcca tggtgcatgc gggcctcatc aactaagggc gtcgacggtc
     8941 cggccgtgac gaaagcgttt tccagcgagg tggagaccgg ttcgcgtcaa gaaaacgcgt
     9001 caactcaaga atcggcgccc cgtttcgatt cgatcgaaac ggaaaaggca ctagcctggt
     9061 gagcgacgtc gaagagaagg ggctcaagat gctcaaggaa tttcgcgaat tcgcgatgaa
     9121 gggcaacgtg gtcgacctcg cggtcggggt catcatcggc gcggcgtttg gcggcatcgt
     9181 cacttcgctg gtcggtgaca tcatcatgcc gatcatcggc gcgattacgg gtggactgga
     9241 tttctcgaac tattttacag gcctgtcgaa ggcagtcacc gccagcaatc tcgccgatgc
     9301 gaaaaagcag ggcgcggtat tggcttgggg caactttctg acgctcacgc tgaacttcat
     9361 catcatcgcc tttgtgctgt tcgcggtgat tcgcttcatc aacacgctga agcggaagga
     9421 tgaagccgcg cccgcgcagc cgaaaccgac ccgtcaggaa gagttgctga ccgaaatccg
     9481 cgatttgctc aaaaagtctt gacatgcgca caggcgggtt gagtgttacc gctctcggtc
     9541 ggagctagat cccaaggcat ttttcatcat ggccgaaaag aacgagcgcg cgatcaaggt
     9601 cgtcgccgaa aatcgcaagg cccggttcaa ctatgcgatc gaggacacgg tggaggcggg
     9661 gatcgcgctg accggcaccg aggtcaagtc gatccgcaac ggcaagacca ccatcgccga
     9721 atcctatgcc gataccaagg gcggcgaaat ctggctgatc aacgccaata ttccggaata
     9781 tctgcaggcc aatcgcttca accacgagcc gaagcggccg cgcaagctgc tgctgcacag
     9841 aaagcagatc aacaaactga tgggcgcggt cgaccgcgac ggcatgacgc tgattccgct
     9901 aaagctctat ttcaacgaac gcggccgcgc caagctcttg ctcgcggtgg cgaagggcaa
     9961 gaagttgcac gacaagcgcg aaagcgagaa gaagcgcgac tggggcaggg agaaaggccg
    10021 cctgctgcgg gcgcggggat agaggttagc ctcatgtata tccgtctttg cgagccgacg
    10081 ggtcggccca aggggcctcc cgatgacggg ctccgcgaag caatccagca tctgggaccg
    10141 caacaactgg attgcttcgt cgcaagtgct cccttgcgcc aacgcttcgc gtttgtcgca
    10201 ggcaatgacg accgatggag aattgcgcga tgaaccaaaa gaatctgatt gaagtcgact
    10261 ggagcaaaat tccggcgccg gccgacgacg gcgcggcggc acatctgaag ggcatgacgg
    10321 tcccgccgat cagcctgatc gcgaccgacg atagctcggt cacgctgtcg gcgctgccgg
    10381 ggcgggtcgt ggtgttcgcc tatcccagaa ccggcgagcc cggcaagatc gcgctggtcg
    10441 atgactggga cattatcccg ggcgcgcgcg gctgcacgcc gcaaacctgc gcgtttcgcg
    10501 acctccatgc ggaattgaag gcgtcaggca cgagatacgt gttcggcctg tcgacgcaaa
    10561 gcaacgccta ccagaccgaa atggcctcgc ggctgcatct gccgttcccg gtcttgtccg
    10621 acgagaagct aacgctgacg catgcgctgg atttgccgac catggaggtg gcgggcctga
    10681 ccatgatcaa gcggctggca ctgatcgtcg atgacgggcg catcacccag gtgttctatc
    10741 cggtgtttcc gccggaccga aacgccggcg acgtgctggc gtggttgaag gaaaacccgg
    10801 tttgacaaat gccgccgtca tccccgggat gaacgcaatt gtgttcaccc gtgaggtgcg
    10861 agcccctctt gggcgagcct cgaacgatga agtgttcagc gcatctggct atccttcgaa
    10921 gcgcacaaga aagcgcgcgc acctcaggat gacggcgcgc gtgtcgcgcg gtcaccagag
    10981 ttgtcaatcc aactccgctc tcaccttcgc aaatatgctg cgaaacatct gtggcgtcag
    11041 cacccgcgta ttggtgttgt agcgcgagca gtgatagctg tcgtagaatt tggtgacgcc
    11101 ggcctgatgc actgcgccat gcgcaaacgg cgccgcggcg gcccgcaagc ccagcgcctt
    11161 cagcgtcgat tcatgcgcga cccggccgag cgccacgatc gcgcgcaggt tcggcatggt
    11221 cgcgatggtc gcgatgagaa attggcggca gacgttgatt tcggccggtt gcggcttgtt
    11281 ctgcggcggc acacagcgga ccgcgttgct gatgcggcaa tcgaccagcg tcagcccgtc
    11341 gtcggggcgc gcctgatagt ggcctttggc aaagccatat tcgagcagcg tcgcatagag
    11401 caggtcgccg gcgtagtcgc cggtgaaggg ccggccggtg cgattggcgc cctgcaggcc
    11461 gggcgccagg ccgacgatca atagccgcgc atcgggatcg ccgaacgacg gcaccggtga
    11521 attgaaccaa tgcggttcgc gtgctcgcgt cgcctcacgg aagcccgaga gccttgggca
    11581 gagcggacaa ttgcgatccg gttcgctgcg gagcagggcg gcggcgagca acggcccgcc
    11641 gcgcgccgga cccttctcaa tcgtcgaatt cgtcgtcatc gcccctcggc gccatggtgg
    11701 tcgcgcgctg caggaattgc ggcgcgtgat gacgcggctc ccgcggcggc ggccgctcgg
    11761 atggatcgcg gccgagcttg gactgcagct ccaccagatc ggtgaagacg tcggcctgcc
    11821 gccgcagctc gtcggcgatc atcggcggct ggctcgaaat cgtcgagacc acggtaaccc
    11881 ggacgccgcg acgctgcacc gcttcgacca gcgagcggaa atcgccgtcg ccggaaaaca
    11941 gcacgatctg gtcgacgtgc tcggcaagct ccatcgcatc gaccgcgagc tcgatatcca
    12001 tgttgccctt caccttgcgg cgaccgctgg cgtcgatgaa ttccttggtc gccttggtga
    12061 cgacggtgta cccgttgtag tcgagccagt cgatcagcgg acggatcgat gaatattcct
    12121 gatcctcaat aatcgcggtg tagtagaacg cgcgcagcag cgtcccgcga ctctgaaatt
    12181 ccttgagcag gcgcttgtag tcaatgtcga agccgagcgt cttggctgtt gcgtagaggt
    12241 tggcgccgtc aataaagagc gcgattttat tcgaagcagg tgacatggtt tctcgcctgg
    12301 ttgttgtttg ttggtcaacg tttttggcgc ggcggcagcg ggccgtgcat tttagcgaat
    12361 gatactgcca aagcccggcg gatcagtctg aacagacgta gaggtaaggg caatagacgt
    12421 catatacttt tgacgtgcaa tgaagcgttt tcttgtccga accgttgttt gtgaaggctc
    12481 aggacactcc ggccggcgag cccccggaca ggttagcagc gccgattgcg ccggccaaat
    12541 cataaattgg tcttgcgaag cgcgtctggt gcctataact aacgcccata atcaacatat
    12601 tcggccccat ttaacggagc gacagtcaat ggcgcgcgtc actgtagaag attgcattga
    12661 taaagtagac aaccggttcg atctggtcct gttggcggcg caccgggccc gaatgatatc
    12721 gtccggttca caactaacgg ttgaccgcga taacgacaag aatccggttg tatcgttgcg
    12781 cgaaatcgcc gattcaacca tttcgcccga ggatttgcgg gaggaactgg ttcattcatt
    12841 gcagaagttc gtcgaagtcg acgaacccga gcccgatacc gtgcctttga tcgggtcggc
    12901 cggcgccagc gtcgacgccg acgacaccga agtcgccgtc gagcggatga ccgaggagga
    12961 gctcctgaag ggtctcgaag gcttggcgcc tccggaagaa cagcccgaag aggacgaata
    13021 gaccggccgg cgtggtaccg ccggtatccc gaacgacctg atttaccaaa aggctcggac
    13081 atctgtccgg gcctttgctt ttgttggcgt tttcgtggtt accatgtgtc catcaccggc
    13141 gcgggcgaat ctgcggcctc tgccttcgat ttgatcggaa ggcaggcggt ttggcgttag
    13201 actgcgatcg gacgtccgag gtccggcgtc ctggccccaa tgggcacgtt gggttgtgtt
    13261 gcgaacaggt tacacgtcga ttccgacatg aaggcacgat gcgatggcgt ttagacgcca
    13321 cagtccctgg caaatgcaag ccgcgaccga ttctgtcgcc gtggcgccgt cctcggctgc
    13381 gccggcgaag ccggccaggt caccgcgccc gcgcatgatg cggcaatacg atctcgtcga
    13441 gcgcgtcagg tcgtataacc ccgacaccaa cgaagaccag ctgaaccgcg cctatgtcta
    13501 cgccatgaag gcgcatggca cgcaaacccg cgcatccggc gacccgtatt tctcgcatcc
    13561 gctcgaggtc gcggcgatcc ttaccgacct caagctcgac gatgccacca tcgtcgcggc
    13621 gctgctgcac gacaccatcg aggataccga agccacccgc gctgaaatcg accacatctt
    13681 cgggcacgag atcggcgcgc tagtcgaggg cctcaccaag ctgaagcggc tggagctggt
    13741 gtcgcgcgag gccaagcagg ccgagaacct gcgcaaattg ctgctggcga tcgccgacga
    13801 tgtccgcgtg ctcctgatca agctcgccga ccgtctgcac aacatgcgca cgctggaatt
    13861 cgtgccgccc acggcacgcc gccggatcgc cgaggagacg ctcgacatct atgcgccgct
    13921 tgccggccgc atgggcatgc aggagatgcg cgaagagctc gaggaccttt cgttccgcac
    13981 gctcgatccg gaagcttacg cggtggtgat gcagcggctg gatgcgctgg ccgagcgcaa
    14041 ccgcaacctg atcggcgaaa tcgagagcca gctcaccaag aatcttcaga acaacggcgt
    14101 gccggcgcat gtctatggcc ggcgcaagca gccgttttcg atctggacca agatggagcg
    14161 caagtcggtc ggcttcgagc aattgtcgga tattttcgga ttccgcgtgg tgatggatga
    14221 cgtcgaagcc tgctaccgcg cgctcggcgt ggtgcacacc acatggccgg tggtgcccgg
    14281 ccggttcaag gattacatct cgacgccgaa gcagaacgat tatcgttcga tccacaccac
    14341 cgtgatcggt cccggcaacc agcgcgtcga attgcagatc cgcaccgagg agatggacga
    14401 gatcgccgaa ttgggcatcg ccgcgcacgc cttctacaag gacggcgtcg gttcgccgac
    14461 cgatttgctg aagcgggaat ccaacgcttt cgcgtggctg cgccacaccg tcggcatttt
    14521 gtcggaaagc gccaatccgg aagagtttct ggagcatacc aagctggagc tgtttcacga
    14581 tcaggtgttc tgctttaccc cgaagggaaa gctgatcgcg ctaccgcggc aggccaatgt
    14641 gatcgacttc gcctatgcgg tgcataccga tgtcggcaac agcgcggttg gatgcaagat
    14701 caacggcaag ttcgcgccgc tgtcgtccga actgcaaaac ggcgacgagg tcgaggtcct
    14761 gacctcggaa gcccagtcgg cgccgccatc ggcctgggaa tcgctcgcgg tcaccggcaa
    14821 ggcccgggcg gcgatccggc gtgcgacgcg cacggccgtg cgcgatcaat atgccggcct
    14881 tggccgccgg atcgtggagc ggctgttcgt ccgcgcaaaa atcgaatacg ccgacgacaa
    14941 gttgaagggc gcgctgccgc ggctggcgcg cgcctcgatc gacgacgtga tggcggcggt
    15001 cggccggggc gaaatgaagg cgtccgacgt cgcgcgcgcg atgtatcccg attacaagga
    15061 agaacgggtc gcgcgatacg gcgcccccaa gaaaagcctg gcggtcaaac tcaagctgaa
    15121 atcgccgccc gatcccgcgc gcagcccgtc ggcgataccc gtgcgcggca tcaattccga
    15181 tttgccggtc aagttcgcgc cgaacggcgg cgcagtaccg ggcgaccgga tcatcgggat
    15241 cgtttcgccg ggggaaggca tcacgatcta tccgatccag tcgccggcgt tgaaggattt
    15301 cgaagacgag ccggaacgct ggctcgacgt gcgctgggac gtcgacgaaa cgacgccgca
    15361 gcgctttccg gcgcggatca tggtcgacaa cgtcaacgag cccggcagcc tcgcccaggt
    15421 ggcgacggtg atcgccgagc acgacggcaa tatcgacaac atcagcatga gccgacgctc
    15481 gccggatttc accgaactga ccatcgatct tgaggtctat gacctgaagc atctcagcgc
    15541 tattatcgcc cagttgcgcg ccaaggccgt ggtcgcacgg gtcgagcgcg tcaatgggta
    15601 gtacagttcg tcattgccgg gcatagtggt ctgccttgcg cagactacgt atacttgtct
    15661 acgctcccgg caatccattc ttttcaagag atggatgcgc gggtcgagcc cgcgcatgac
    15721 agccggagtg ttcagccgcc ctctaaattc cagtgcgagt ccgttatgcc caaacctcca
    15781 ccgctccgcc tcggcgtcaa tgtcgatcac gtcgccaccc ttcgcaacgc gagagcaggc
    15841 cgcaatccgg atccggtgcg cgccgcgctg gttgcgatcg cggccggcgc cgacggcatc
    15901 accgcgcatc tgcgcgagga ccgccgccac atccgcgacg aggacatggc gcggctgaag
    15961 gcggagatct ccaagccgct gaatttcgag atggcggcga ccgaagacat gctgcggatc
    16021 gcgctcgcga ccaggccgca tgcggtgtgc ctggtgcccg agcgccgcga agaactcacc
    16081 accgagggcg gtctcgacgt ggtcgcgcag cacaatgcgc tggcgccgtt tattgccaga
    16141 ttgaacgatg ccggcgtgcg ggtgtcgctg ttcatcggcg ccgagccgcg gcagatcgag
    16201 atggcggcaa agctgcgcgc gccggtgatc gagattcaca ccggcgcctg gtgcgacgcc
    16261 gtggtcgatg gtcatgacgc cagggccgaa tcagaatggc agcggatcgt cgcaggcacg
    16321 cgcctggcgc gctcggccgg gctggaagtc catgccggtc acgggctcga ttatgtgacc
    16381 gcggaaaaga tcgcaggctt accggagatc gtcgagctga atatcggtta ctacatgatc
    16441 ggggaagcgt tgttcacagg cctcggggaa acggttcgcg cgatgcgggc ggcgatggac
    16501 cgcgggcgca acatggccgc gggtcaatcg cccgacggcg agaagccagc gggcaagaca
    16561 tgatcctcgg catcggctcc gacctgatcg acatccgccg catcgccaaa gtcatcgagc
    16621 gtcacggtga ccgctttctc gaccgcattt tcaccgaggc cgagcgcgcc agggccgcgc
    16681 gccgcgccaa aagcgagaag atggtggtcg caacctacgc caagcggttt gccgccaagg
    16741 aagcctgttc caaggcgctc ggcaccggca tccggcgggg cgtgtggtgg cgggatatgg
    16801 gggtggtgaa cctgccgggt ggccgtccga ccatgcaact gacgggcggg gcgctggccc
    16861 ggctcgaggc gatgacgccc ccagggcacg aggcgcgggt cgatctgagc attaccgacg
    16921 actggccgct ggcgcaggcc tttgtcataa tttcggcggt caatgtcggc aaaccgtgac
    16981 accctcgaag gccgtctccg gcggcttgcc gcaaaactaa aaatctaaga catgtcaacg
    17041 gattagcaag ttttgatgcg gtcgttgatt gcgtggccgc gaacaaccgt ctaaaacgct
    17101 tggcgtcttg atccgaagtt cgtttatatc gctgcacctt ccgagcgact tcggattcca
    17161 aggttaccga aaaactctga ttctagtgtg gtttaggtcc ggaagctcgc tcttggaagc
    17221 cttcagcgag ggtttaggag aacttccggg cctaagccac cctagggaga acatctagtc
    17281 gggcgaattc gggcttgcgc caaaattcgc tctcaatatc agcatcaaga ccattttgtt
    17341 ggcgcgaagc gaggggcgat tcgcgcgcgc aaaatgttct gccaccgtgc ggcggtcgcg
    17401 aagggaattg ggaaaatcga tgagcgtgac atccgggaca aaatctgaga gcggcttcgg
    17461 cgaaaccgtt cgcgtcgtca ttcacgccct gttgatcgcg ctggtgatcc gcacatttct
    17521 gttccagcct ttcaacatcc cctcgggatc gatgaaggca acgcttctgg tcggcgacta
    17581 tctgttcgtc tcgaaatact cctacggcta cagccattac tcgattccgt tgtcgccgcc
    17641 gctgttttcg ggacgcatat tcggttccga gccggcgcgg ggcgacatcg tcgtcttcag
    17701 gctgcccaaa gacgatacca ccgactacat caagcgcgtg atcgggttgc cgggtgatcg
    17761 catccagatg aaggatgggc tgctctacat caatgagaag ccggtgacac gcgagcgcct
    17821 gagcgatttc gtcggcgaag acccttgcgg ctccgacgcg acttcgcggg tcaagcgctg
    17881 gaaggagacg ctgccgaatg gcgtcaccta tcagtcgctg gattgcgtcg acaacggttt
    17941 ctacgacaac accagtgtct acaccgtgcc gcccggccac ttcttcatga tgggcgacaa
    18001 cagggacaac tcgaccgaca gccgggtgct gtcggcggtg ggttacgtgc cgtttgagaa
    18061 tattgtcggc cgcgcccaga tgattttctt ctcgattgcc gagggcgaac atgcctggat
    18121 gctctggcgc tggcccaccg cggtgcgctg gaatcgcata ttctcaattg tgcgatgaac
    18181 gacgaaacag ctgatatccg gatcttgtcg tcatcaggcg acgcaggcca tcagcttgag
    18241 agggcgagcg aacctgcggc gaagaaaaag cgcagcaaga tcagcgccaa ggccgccacg
    18301 gcggcgatcg aggcgcggat cgggcacaaa ttcgccgatg ccaccttgct ggcgacggcc
    18361 ttcacgcatg tttccgcgct gaaatcagcc cgcaagcgtg gtgacagtta ccagcgcatg
    18421 gaatttctcg gcgaccatgt gctcgggctg atcgtttccg acatgctgta tcgcgccttc
    18481 ccgaatgccg atgaaggcga actgtcgaag cggctcgccg atctcgtgcg caaggagagc
    18541 tgcgcggatg tcgcgaagtc gctcgggctg gtcgacgaca ttaagcttgg cgcggtcggc
    18601 gccggtgcgg gcgcgcgctt acgcaaatcc gtgcttggcg acatctgcga ggcggtgatc
    18661 ggtgcggtgt ttctcgacgg cggctacagt gctgcggcgc agttcgtcga gcgcaactgg
    18721 accgagcgga tgcgcaagcc gcggcggccg ctgcgcgatc ccaagacggt gctgcaggag
    18781 tgggcgcagg gcaaggggct gccgacaccg gtctatcgcg aaatcgagcg caccgggccc
    18841 catcacgatc cgcagtttcg cgtcgcagtg gatttgccgg gattggcgcc ggccgagggc
    18901 gtcggcggca gcaagcgcgc cgccgaaaag gtcgcggcgt cggtgatgat cgcgcgcgaa
    18961 ggcgtcggcg gcggcagcaa tgatggatga actccctgac gatgccgcgc cggtcgcgac
    19021 gcgctgcggc tttgtcgcgc tgatcggcgc ccccaatgtc ggcaaatcca cgctggtcaa
    19081 tgcgcttgtc ggctccaagg tcacgatcgt ctcgcgcaag gtgcagacca cccgcgcgct
    19141 gatccgcggg atcgtgatcg acaacaacgc ccagatcatt ctggtcgaca cgcccgggat
    19201 tttcctgccc aagcggagac tggatcgcgc catggtgtcg accgcatgga gcggggcgcg
    19261 tgacgccgat ctggtctgcg tgctgctcga tgcgcgcgaa ggcattgacg aggaagccga
    19321 cgccatcctc accaaggtgg cgaccgttcg tcacgagcga attctggtgc tcaacaagat
    19381 cgatctggtt ccgcgcgaaa aactgctggc gctgaccaag gccgccaatg accgcctgag
    19441 tttcgaaaag accttcatgg tttcggcgct gtccggcgat ggcgtcgccg atctcaggca
    19501 tacgctggcg gcgatggttc cgccggggcc gtttcactat cccgaggacc agatgtcgga
    19561 cgcgccgatg cggcatctgg cggctgaaat cacccgggaa aaaatcttcc gccagctgca
    19621 tcaggaactg ccgtatcaat ccaccgttga gaccgatagc tggaccgagc gcaaggacaa
    19681 gtccatccgc atcgagcaga cgatctttgt cgagcgcgaa agccagcgca agatcgtgct
    19741 cggcaagggt ggcgccacca tcaagtcgat cggcgcggac tcacgcaagg aaatcgccga
    19801 gatcgtgggc gtccccgtgc accttttctt gttcgtgaag gtgcgcgaaa actggggcga
    19861 cgatcccaac cgttaccgtg aaatgggact tgaattcccg aaggaatagc tggcgccctc
    19921 gatgaacgtg cccaaaaacg tcgtttggtt tgaagtgctt ctgtatatgt cactaacgct
    19981 cgacgcgctc tcggtcgcgt tccaggatcg aacgccgagc gcggagatga cggaacagat
    20041 gatcacggtc gcgaccgtga tggcggcctt cctgatcctg ctcctggtct tcttcgtctg
    20101 gctcgccgca caacggcgaa aaaattggcc gcgctgggcg ctggctgccg caggggtgct
    20161 ttcgatcata cagttggtgc agatcatcgc cgacagcggc ctgcagctcg atagcggcat
    20221 cgagatcgtt tcatgcatcc tgacggccgc cgggctgtat ttttccttta ccggcgacgc
    20281 ccagggctgg ttcaacgggt aatagttact catcgtaatt ccgggttcga tgctttgcat
    20341 cgccccggaa tgacggtggg agagatagct gcgcccagca acggaatgct gtacactcca
    20401 ctccatggaa tggaccgacg agggcatcgt gctgggggtg cggcggcatg gcgaatcctc
    20461 agccatcgtc gaactcttga cgcgcggtca cggccgccac ctcggcctgg tgcgcggcgg
    20521 ggcgggttcg cggatgcggc ccttgctgca gcccggcaac agcgtgcgcg cggtgtggcg
    20581 ggcgcggctc gacgagcatc tcggctatta ccagatcgag ggcacgagac tgcgcgcggc
    20641 caccgtgctg gcgtcgtcgc acgcggtcta tggcgtgacg catctggctt cgctggcgcg
    20701 gctgttgccg gagcgcgatc cgcacgaaga catctacgag atgctggaac gaaccctcga
    20761 cgacttcgac gacatcggcg aagccgcggt acatctggtc aagttcgaac tggcgatgct
    20821 cgccgaactc ggttttgggc tcgatctgga aacctgcgcc gccaccggcg ggacctccga
    20881 gctgatctac gtgtcgccga aatccggcgg cgctgtgtcg cggcaggccg gcgatccctg
    20941 gcgcgaccgg ctgctgcggt tgccgccgtt cttgcgctcg tccggcggcg gcccgaacgg
    21001 gtggtcggat caggatcttc aggacggttt tgcgctgaca ggcatgttcc tcttgcgcca
    21061 cgtgctggag ccgcgcgggc agggccactc cgacgcccgc gacggtttta tcaacgcggt
    21121 gacgaaacac cgggcgagaa tggcgcttcc gtagagccga gcccgatgat ggcggcgctg
    21181 gtgcggaagg acgagtccgc gccgcaacaa agaagcaaca atagcggggg taattccggc
    21241 gcggtgcagt aggaccttgt ttttcaagct gcgagtcagc taattttcct tattcgtttt
    21301 cggcgctaaa tgcgtgttcc gtcaatggaa cttggctgcg tcgaacaatt tgaactgggg
    21361 caagaggggg ttggataatg tggatacgct ggacgtgcgt cgctgcgatt tgcctggggc
    21421 tgacgtcgca ggccgaagct gccgagcact ccggactggt tcatcgcgcg tcctgcgccg
    21481 ttgtccggtt ctacgtcgcc aagttctcgg cgttcgccgc cgagacatgg gcgcgcagtc
    21541 acggggccac cgaagccgaa attgacgccg ctcggcactg cctcaaagac gcacccgcgc
    21601 agaccgcgca ggccagccaa tactcaaagt tctgagcacg cctaaatgag gcgcgtgcag
    21661 agtccctttg caatcctatg ggttgagtga aggagactgc ccggatgggc ccatcatgac
    21721 tccgatgggc acatcggaaa tcaggccact ctcaaagatc gaattaggcc actggaggtc
    21781 gaaacgggcc agtaccaacc ggccccaaga atattccgtg aaaggggtgt tctaccagtg
    21841 gaacaccctg agatagcgcg tctctgctag actgcggcca tgagcgattc gaccccatac
    21901 aactacagat gcccaaacct ggaatgtcgg gccgaatacg ttgccgtgca gcggaccgac
    21961 gcttccctgc gcaagccgag gtgcatcgaa tgcgcaacgc cgttccttgc catgcagaag
    22021 ggccggtata tccaatatga ggccgcgtgg cccgtcaccc tcgcgctgcc agacttggcc
    22081 tgatggccgg gagtcaggtg gcgatcttag tcgcggaaac gatcctccag caacggcggg
    22141 agcatcttga tgatgatcga tcccaagtcc aggctgatcg cagctctcgg caacgcgcag
    22201 gaatacattg atctcgcggc cagcgccaag gatcgtggcg agcgcgagtt ctacgaacgc
    22261 attgcagagc tgtacataag gatcgcgcag gagctggatg cgctggtcga gggatagggc
    22321 gtcgcctgcc gcaacgggcc gctaacggct cctgcatttc gcggattcaa acccggcaat
    22381 tcgcagcctg gcggggggac gcccctggaa agctcgcgca atgtgagtgg gatcacagca
    22441 cccgggctca ctccgcctat cctccgagga cgataatggc gggagtattc aatgtacgac
    22501 agaaccgact tggtgacgat tgccacggca gtctgcctgg cgggctatgc cgcgatcgca
    22561 ctgtggtatg tgacgttatt cgatatcgga ctcacgcgcg agggggtccg cagcggggcg
    22621 acggtctgcg caatcctgtt ggccgcggtg gtcgcgatgg atttcttggc caggaagctc
    22681 ggcaagcgcg aatgagcctc ggaacgcggg gagcgcatcg cccagaccgc gcaggccggc
    22741 caatcatcaa cgtttccgcg cccgcctgaa aatgaggcgc gtgccgcccc tgattcgcgg
    22801 cttgcccgat tcgcctccag cgtttaactc ctccccatgg gaaaaagact gattccgccg
    22861 gagccggccg aaatccacga ggtgatgctg cgggatgcgc tcgaggagcg ctatcttcag
    22921 tacgcgctgt cgaccatcat gcaccgcgcg ctgccggacg cccgtgacgg gctgaagccg
    22981 gtgcatcgcc gcatcctcta tggcatgcgc ctcttgcggc tcgaccccgg cacgccattc
    23041 aaaaagtccg ccaaaatcgt cggcgacgtg atgggctcgt tccatccaca tggcgaccag
    23101 tcgatctacg acgcgctggt gcggttggcg caggatttcg cctcgcgcta tccgctggtc
    23161 gacgggcagg gcaatttcgg caatatcgac ggcgataacc ccgccgccta ccgctatacc
    23221 gaagcccgca tgaccgaggt cgcgcggctg ctgctcgaag gcatcgacga ggacggtgtc
    23281 gaattccgcc ccaattacga cggccagtcg aaagagccga tcgtgctgcc cggcggattt
    23341 ccgaacctgc ttgccaacgg ctcgcagggc atcgcggtcg gcatggcgac ctcgatcccg
    23401 ccgcacaacg cggccgaact ctgcgatgcc gcgctgcatc tgatcgacaa gcccgaggca
    23461 aagtcgaagg ccctgctgaa gtgggtcaag ggtccggatt ttccgaccgg cggcatcatc
    23521 gtcgattcca aggagagcat cgcggaagcc tatatgaccg ggcgcggctc gttccgcacc
    23581 cgcgccaggt ggagccagga agagggtgcg cgcggcacct gggtcatcgt cattaccgaa
    23641 attccctggc tggtgcagaa atcccggctg gtcgagaaga tcgccgaact gctcaacgac
    23701 aagaagctgc cgctgatcgg cgacgtccgc gatgaatccg ccgaggacat ccgcctcgtc
    23761 atcgagccga aatcccgcgc ggtcgatccc gaattgctga tggaatcgct gttccggctg
    23821 accgagctgg aaagccggat ttcgctgaac ctcaacgtat tggtgaaggg ccgcatcccc
    23881 aaggtgcttg gccttgccga gtgcctgcgc gaatggctcg accatctgcg cgacgtgctg
    23941 ctgcgccgct ccaattaccg caaggtgcag atcgagcacc gtttggaagt gctcggcggc
    24001 tatctgatcg cctatctgaa catcgacaag gtgatcaaga tcatccgcac cgaggacgag
    24061 ccgaagccgg cgctgatcaa ggcattcaag ctcaccgaaa tccaggccga cgccatcctc
    24121 aacatgcgcc tgcgcaattt gcgcaagctc gaggaattcg agatccgcac cgaggacaag
    24181 aatcttcgcg ccgaactgaa gggcatcaag gagctgctcg gctccgagac cgcgcaatgg
    24241 tccaaggtcg gcgagcaggt ccgcaaggtc cgcgacctgt tcgggccgaa gacgccgctc
    24301 ggcaagcgcc gcaccacctt cgccgacgcg ccggagcacg atcttgccgc gatcgaggaa
    24361 gcctttgtcg agcgtgagcc ggtcaccgtc gtggtttccg acaagggttg ggtgcggacg
    24421 ctgaagggcc acgtcgccga tctctcaggg ctgaccttca agaccgacga ccagctcggc
    24481 tctgccttct tcgcggagac cacgtcgaag cttttgttgt ttgccaccaa cggccgcttc
    24541 tattcgctcg aagtgtcaaa actgccgggc gggcgcggcc atggcgagcc gatccggatg
    24601 ttcatcgata tggagcagga tgcatcgatc gtgtcgctgt tcgtcaacaa gggcggacgc
    24661 aagttcctgg tcgcgagcca tgaagggcag ggctttgtcg tcaacgagga tgattgcgtc
    24721 ggcaacaccc gcaagggcaa gcaggtgctg aatgtcgaga tgccgaacga agcccgcgcc
    24781 ttgacgaccg tgaccggcga caccgtcgcc gtcatcggcg acaaccggaa gatggtgctg
    24841 tttccgatcg atcaggtgcc ggagatggcg cgcggccgcg gcgtgcgcct gcagaaatac
    24901 aaggatggcg gcttgtccga tatcgcgacg ttcgatgcca aggatggcct gagctggaag
    24961 gactccgccg gccgcgaatt cagcgccacg atcaaggagc tggcggactg gcgcggcaac
    25021 cgcgccgacg ccggccgtct gccgccgaag ggttttccga agtcgaacaa gttcggacgc
    25081 gggatcgagt agggctcaag cattgcggtt cccgccgccg cacaccgccg cctctttcgt
    25141 cagcacgagt gtggcttttg tctgcccggc ctcccagcac gaaaattgca tggaacaatc
    25201 cagcgcggga ttgagagcca ttccatgaac ctcatcaatc tggatatcag gatgctccgc
    25261 tccctgactt cggtcgtcga gaccggaagc attaccgaga ccgcgcgacg gctcgggcgg
    25321 actcagccgg cgatcaccct tcagttgcag cggctcgaag aactcacggg gaaggcgatc
    25381 tttacccatg aaggccgcaa gctgtttctc accgccgacg gcgagacggt gctttcctat
    25441 gccaagtcta tattgggatt tcatgacgag ctgctgtccc aactggcctc cccggatatc
    25501 gagggacatg tcgttctcgg cacgccggat ctctacgccg cattcatgct gccttcgatc
    25561 ctcaagattt ttcgaaagac ctttcctcgg gtgcagatcg aattgaactg ctcgctgtcg
    25621 acgccgctgg tcggatgtgt gaagcgtggc gaagtcgata tcgccctggt gacgcggatg
    25681 aatgatttca ccggcggcca ggtggtgcgg caggaacaat tgatctggat ggcgggggaa
    25741 caatcgacgg cgcacaagga aaccccgatc ccgctcgcgc tgctgccgcc gggcaacatc
    25801 tatcgcgacc acgccctgga acgactggaa gcgtcacgcc tgagatggcg tatcgcctgc
    25861 atcagcgata gcgtcggcgg gttgcaggcg gcggccttcg cgggaatggc ggtgaccgtg
    25921 cttggccgca gcgcgctggt gcgcaacatg cgcgaaatcg gggtcaagga aggcttgccg
    25981 gcgctgccaa aggtcgatct tctgctttac aaatcgccga ccgcaacttc caaggccgcc
    26041 ggagccctgc acgagtatct cgcgcattat ctcaatctcg acgacgagct gttgcttgga
    26101 aaagagttgc cgctgacggc gaccgatgcc gacgcagtaa gcctgtcagg tcatgcgggg
    26161 acgaaactga gcccagggat gcgcccattc aaatgcggcg gcgatccgca ggatcacccc
    26221 ctcgtcgagc cacggcgcga caatctgaag tccgatcggg aggccatcgg tgtcgaaccc
    26281 gcagggaatc gtcgcagccg gcaatccggt caggtttatc ggccacgaga acggtgacca
    26341 gccgaggtgg cgatcgacgg cgacaccggc aacgcgatcg acacccagcg ttcccgctgg
    26401 gaacgctgtg atggcgacgg tcggggtcac cagcgcatcg accgtttgaa ataattcctg
    26461 gaacgacgcg cgcaggctgt tgcgccgata acccgcctcg acatagtcga caccgagata
    26521 gccgcggccg cgcgcgatga cttcgcggta gtcccagtcg gacaaggcga gcgcatcggc
    26581 gccgagaccc gcgaccgccg ccgcctgctc ggtataggcg atcggcttca agatgcgatc
    26641 cagcatgtcc ggagcgaatg cgacgcagtc gggcaatatc tcggcgccaa gcgatgccag
    26701 cgaagccagc gctgattgga atgccccgcg cacgtcgggg tcgaccgcgg
//
LOCUS       DATNOI010000595         4054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1500604,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000595 DATNOI010000000
VERSION     DATNOI010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500604"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VN968_24185"
     CDS             <1..247
                     /locus_tag="VN968_24185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32364.1"
                     /translation="RLFLPIKTMADGELHDGGSTSFTLVFLLWTSGACSRSHRLSVAV
                     LLAVRMFGWFRLGDRVTHTPNIFPAGYEFEGDRGAIS"
     gene            complement(273..557)
                     /locus_tag="VN968_24190"
     CDS             complement(273..557)
                     /locus_tag="VN968_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32365.1"
                     /translation="MAQLPNLPQQLRSTQVIVRFGLRMIILTLFATFGSIGFGRSLAA
                     LLWMSTILSAVIGAMKREPPLDRALNHWDEMVVYAALCCLASGFNQAALF"
     gene            complement(654..1130)
                     /locus_tag="VN968_24195"
     CDS             complement(654..1130)
                     /locus_tag="VN968_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32366.1"
                     /translation="MTSMLLLGEHREPRELSDRLRDAHAATEDLVAEVIGETCRRFPS
                     VGQSEKTTRIERLIQAGAWTDAALALIDLELPQWQVRRIAYDEGEWYCALSRERELPD
                     WLDQSIEARHPDLPLAILSAFVEAQRMAAPSSRTSVPVVPRAASTSCEPVLTDNFT"
     gene            1576..1665
                     /locus_tag="VN968_24200"
     CDS             1576..1665
                     /locus_tag="VN968_24200"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF011342.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HXL32367.1"
                     /translation="MIFDYSLAAIVSAGLLFYLTYALLRPERF"
     gene            1709..3412
                     /gene="kdpA"
                     /locus_tag="VN968_24205"
     CDS             1709..3412
                     /gene="kdpA"
                     /locus_tag="VN968_24205"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HXL32368.1"
                     /translation="MTAIGWIQIILYCAIIVALVKPLGWYMTRVFNGERTFLSPILRP
                     VEVGLYWLGGVDERREQHWLTYTVAMLLFHVGGFLIIYTVMRLQAVLPFNPAEQSAVA
                     EDLSFNTAISFITNTNWQNYGGESTLSYLVQMLGLTHQNFLSAATGIALAVALIRGFS
                     RSSMRTIGNFWVDVTRCTLYVLMPICVVYTLFLVWQGIPQTLGAYVDATTLEGAKQTI
                     AVGPVASQIAIKMLGTNGGGFFNANAAHPFENPTALSNFVQMISIFALGAALTNVFGR
                     MVGNQRQGWAVLAVMGVLFISGVIVTYWAEANGTTALQALGLTGGNMEGKEVRFGIVA
                     SSLFAVITTAASCGAVNAMHDSFTALGGMIPLINIQLGEIIVGGVGAGLYGMLIFVVI
                     SIFVAGLMVGRTPEYVGKKIEAREVKMAMLAILVLPLMYLGWTAVAVVLPPAVASMAN
                     AGPHGFTEVLYAYTSQTGNNGSAFAGLTGNTPFYNITGGVAMFVGRFFMIVPAMAIAG
                     SLAGKKSIPPSAGTLPTTGGLFVGLVVGVILIIGGLTFFPALALGPIVEHLAMNANTL
                     F"
     gene            3435..>4054
                     /locus_tag="VN968_24210"
     CDS             3435..>4054
                     /locus_tag="VN968_24210"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492775.1"
                     /note="One of the components of the high-affinity
                     ATP-driven potassium transport (or KDP) system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HXL32369.1"
                     /translation="MEVVKMNKRMPVSAMLDPKIVMPAIGSAFVKLDPRVMIKSPVMF
                     VVEVVAALTTVIFLRDLVTGGANLGFTFQIIQWLWFTVLFANFAEAVAEGRGKAQAES
                     LKKTRTESQAKLLTGSDKNYRLVSGTSLKVGDIVLVEAGDNIPSDGEVIEGVASVNEA
                     AITGESAPVIRESGGDRSAVTGGTQVLSDWIRVRITAAQGSTFIDR"
BASE COUNT          726 a         1293 c         1225 g          810 t
ORIGIN      
        1 gcggctgttc ctgccaataa agacgatggc agatggcgag ctgcacgatg gaggttcgac
       61 gagcttcacg ttggtattcc ttctgtggac gagcggcgcg tgctcccgaa gccaccgact
      121 gtcggtcgcc gtgttgttag ctgtgcgaat gttcggatgg tttcgtctgg gagaccgcgt
      181 tactcatact cccaatatct tcccggccgg atatgaattc gagggcgacc ggggagcgat
      241 ctcataggcg ccgcataagg tcgcccaccg cttcaaaaaa gggccgcctg gttgaatccg
      301 ctggccaggc agcagagcgc cgcatagacc accatctcat cccagtgatt gagcgcccgg
      361 tccagcggcg gctcgcgctt catggcgccg atgacggcgc tgaggatggt ggacatccac
      421 agcagcgcgg caaggcttct gccgaagccg atgctgccaa acgttgcgaa cagtgtcaga
      481 atgatcattc gcaggccaaa gcgaacgatc acctgggtcg atctcagttg ttgcggcaga
      541 tttggaagtt gcgccaagtg tcaataccct gacggatgcg ccttgcgggg tccctcggac
      601 gcgatcgcag gcatagggac gcagcaagct gcgacatcag ttggttcgcg ccttcaggtg
      661 aaattgtcgg tgagcaccgg ctcgcaagac gtgctcgcgg cgcgcggcac cacggggacg
      721 ctggtcctgc tcgacggcgc ggccatgcgc tgggcttcta cgaacgcgct caaaatcgcc
      781 agcggtaaat ccggatgacg ggcctcgatc gattgatcga gccaatccgg aagctcgcgc
      841 tcgcgcgaca gcgcgcaata ccactcgccc tcatcatagg cgatgcggcg aacctgccat
      901 tgcggcagtt ccagatcgat cagcgccagc gcggcatcgg tccaggcgcc ggcctggatc
      961 aatcgttcga ttcgcgtcgt cttctcggac tgtcccaccg aaggaaagcg ccggcaggtt
     1021 tcgccgataa cctccgcgac cagatcttcc gttgccgcat gggcgtcgcg aagccgatca
     1081 ctgagttcgc ggggttctcg gtgttctcca agcagcagca ttgacgtcat cccacataag
     1141 gcccggccgc gatcagcagc cgcaccggcc aagatggaca aaacgccatt tgaaaacgag
     1201 atggggcagg gccccaagat atagggattt cataagtgcc ggcggggaag gttaaacaac
     1261 gcctttcggc cggtcccggc gttcgccgga acgacggcac ttatgagatt cctataacgt
     1321 tctcattgct tccatctcga aaatctatgc gccgggtggc acttcccacc caacggaagc
     1381 gagaaggtca ctcggccggc ccgctgatca cgacgcatag gagcacctca tgctggacat
     1441 catcatgctg gccctcggcc tcggcttctt cggggtggcg gtgggataca cctacgcctg
     1501 cgaacgactt tagagtctga cgcgttttct tcacgcgaat ggcttcgctt gaaaacgctc
     1561 tggaggagca cacccatgat cttcgattat tcgctcgccg ccatcgtctc cgccggcctg
     1621 ctgttttacc tgacctacgc cctgctgcgg cccgagcggt tctgaccgca tcgcacatcg
     1681 aacccgcgtt aactgaaggc atacgaccat gaccgccatt ggctggattc aaatcattct
     1741 gtactgcgcc atcatcgtcg cgctggtgaa accgctcggc tggtacatga cgcgcgtctt
     1801 caacggtgag cgcacgttcc tttcgccgat cctgcggccg gtcgaggtcg ggctctactg
     1861 gctcggcggc gtcgacgaga ggcgcgagca gcattggctg acctacacgg tcgccatgct
     1921 gctgtttcat gtcggcggct tcctcatcat ctatacggtg atgcggttgc aggcggtatt
     1981 gccgttcaat ccggccgaac aatccgccgt cgccgaggat ctgtccttca acaccgcgat
     2041 cagctttatc accaacacca attggcagaa ttacggcggc gaaagcacgc tgtcctatct
     2101 cgtgcagatg ctggggctga cgcaccagaa tttcctgtcg gcggcaaccg gtatcgcgct
     2161 cgcggtggcg ctgatccgcg gcttctcccg ctcgtcgatg cgcaccatcg gcaatttctg
     2221 ggtcgatgtc acgcgttgca cgctctatgt cctgatgccg atctgcgtgg tctacacgct
     2281 gttcctggtc tggcagggca ttccgcaaac gctcggcgcc tatgtcgatg ccacgacgct
     2341 ggaaggcgcc aagcagacca tcgcggtcgg ccctgtggcc tcgcagatcg ccatcaaaat
     2401 gctcggcacc aatggcggcg gcttcttcaa cgccaatgcc gcgcatccgt tcgagaaccc
     2461 gaccgcgctt tccaatttcg tacagatgat ctcgatcttc gcgctcggcg ccgcactcac
     2521 caacgtattc ggccgcatgg tcggcaacca gcgtcagggc tgggcggtcc tggccgtgat
     2581 gggcgtgctg ttcatatcgg gcgtcatcgt cacctattgg gcggaggcca acggcaccac
     2641 cgcgcttcag gcgctcgggc tgaccggcgg caacatggaa ggcaaggaag tccgcttcgg
     2701 cattgtcgcc tcctcgctgt tcgcggtcat caccacggcg gcgtcctgcg gcgccgtcaa
     2761 cgccatgcac gactcattca ccgcgcttgg cggcatgatt ccgctgatca acatccagct
     2821 cggcgaaatc atcgtcggcg gcgtcggcgc cgggctctac ggcatgctca tctttgtcgt
     2881 catctcgatc tttgttgccg gcctgatggt tggccgcacg ccggaatatg tcggcaagaa
     2941 gatcgaggcg cgcgaggtca agatggcaat gctcgcgatc ctcgtgttgc cgctgatgta
     3001 tctcggctgg accgccgtgg ccgtagtgct gccgccggcg gtggcctcga tggccaatgc
     3061 ggggccgcac ggctttaccg aggtgctcta tgcctacacc tcgcaaaccg gcaacaatgg
     3121 ctcggccttc gccggcctca ccggcaacac ccctttctat aacatcaccg ggggagtcgc
     3181 gatgttcgtc ggccgcttct tcatgatcgt gccggccatg gcgatcgcgg gttcgctggc
     3241 gggcaagaag tcgatcccgc cgtcggccgg cacgttgccg acgacgggcg gcctgttcgt
     3301 cggcctggtc gtcggcgtca tcctgatcat cggcggtctc accttcttcc cggcgctcgc
     3361 gctcggcccg atcgtcgaac atctcgcgat gaacgccaac accctgttct gatcgctcat
     3421 tcggagttac gtccatggaa gtcgtcaaaa tgaacaagcg catgccggtc tcggcgatgc
     3481 tcgatccgaa gatcgtgatg cccgcgatcg gttcggcctt cgtcaagctc gatcccaggg
     3541 tcatgatcaa gagcccggtg atgttcgtgg tcgaggtcgt ggccgcgctg accacggtga
     3601 tcttcctgcg cgacctggtg accggcggcg ccaacctcgg cttcaccttc cagatcatcc
     3661 agtggctgtg gttcacggtg ctgttcgcca acttcgccga agcggtcgcg gaaggccgcg
     3721 gcaaggcgca ggccgaatcg ctgaagaaga cgcgcaccga gagccaggcc aagctgttga
     3781 cgggttccga caagaattat cgtctcgtgt ccggcaccag cctcaaggtc ggcgatatcg
     3841 tcctggtcga agccggcgac aacattccct ccgacggcga agtgatcgaa ggcgtcgcgt
     3901 cggtcaacga ggccgcgatc accggcgagt ccgcgcccgt gatccgcgaa tccggcggtg
     3961 accgctcggc ggtcaccggt ggcacgcagg tgctgtccga ctggatccgc gtccgcatca
     4021 ccgcggcgca gggctcgacc ttcatcgatc gcat
//
LOCUS       DATNOI010000596         9707 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_429599,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000596 DATNOI010000000
VERSION     DATNOI010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9707
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429599"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1092)
                     /locus_tag="VN968_24215"
     CDS             complement(37..1092)
                     /locus_tag="VN968_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HXL32370.1"
                     /translation="MLEAIRRAMSFLRQKQILHKFGVVVSITVIGIACYVLYHMLRGI
                     DANEVIEAIKQTESRQIVLAGLFVTAGYFTLTFYDLFAVRAIGRSDIPYRINALAAFS
                     SYSIGHNVGASVFTGGAVRYRIYSAWGLNAIDVAKICFLAGLTFWLGNAAVLGLGIAY
                     HPEAASAIDQLPPWLNRIAAFAIIVGLVAYVVWVWTQPRQVGRGPWTVVLPGGPLTLL
                     QIAIGIVDLGFCALAMYVLVPDEPNLGFVVVAVIFVSATLLGFASHSPGGLGVFDAAM
                     LVGLWQMDREDLLAGMLLFRVLYYLAPFVISVILLTLREVILGARLKRLRHATAGMDA
                     KPKREAVYVRERGDSGG"
     gene            complement(1350..1865)
                     /locus_tag="VN968_24220"
     CDS             complement(1350..1865)
                     /locus_tag="VN968_24220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXL32371.1"
                     /translation="MTPSAHYRMFGHYNAWANGRLYDSVARLSTEQYRADRGAFFKSV
                     HGTLNHLLVTDRIWMQRFTGEGEAPNRLDAILFETFDALRAAREAEDRRIVGFVDGLD
                     EGRIAGTIKYRRVSSPEEFEQQLAPALAHWFNHQTHHCGHVHALLTGLTGEAPELDLL
                     IFQRRSGKPAA"
     gene            complement(1901..2197)
                     /locus_tag="VN968_24225"
     CDS             complement(1901..2197)
                     /locus_tag="VN968_24225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32372.1"
                     /translation="MAYLVTFHWGWLLASLLLGFAMGWIAVVHRGPGVSKRTSLWLAV
                     LVAALVAAALARLVPGRPGYWLDLGLIMFACYLAGCALGSWLRDRVLSRHAPAA"
     gene            complement(2205..3599)
                     /locus_tag="VN968_24230"
     CDS             complement(2205..3599)
                     /locus_tag="VN968_24230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXL32373.1"
                     /translation="MHGLFRWRSKWWPGLIPLAIFWAIAAWTNTSTVESDLAARSTAA
                     LKDTVLDKTRIAVAGRDVTLTADAFSEDGRSRAAASVQAVPGVRRVNDETRLVPEARP
                     FVWSAERDVVRVTLGGSAPLPASKAKLLEAARASLGGVEVADQMNLSRGAPPRFDDAA
                     SLLLDQIGKLKDGKITISDGKVSLSGMARELGGREAIAAALKTLPEGFSVAANEIKAP
                     PYIFQAYKDPVAVTLTLTGYVPDNNVHAALAAAAGRKFFSEKVVDNLKASVGAPSGFA
                     NAVMPALGALSRLSTGTLVVSDREVKLSGDALYDAAAVQMRAGLGKDFPQSWQFKAEI
                     SVKPQAAPVDATVCQQLFSDLLAKGKIRFESGRATIDPDSLGLLDRLVETALRCPAAN
                     IEIAGHTDADGEDGFNQMLSEKRAQAVADYLVKAGLPASQFTATGYGSTQPVASNDTD
                     EGKAQNRRIDFLVR"
     gene            3767..4825
                     /locus_tag="VN968_24235"
     CDS             3767..4825
                     /locus_tag="VN968_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXL32374.1"
                     /translation="MKQFRNHVIRAGLGALYFSGAHHLLRPIFAGVGAIFMLHHVRPR
                     RDTEFQPNRHLEVAPEFLRTMLAHLRARGVDIVTMDELHQRLIDGNFSRRFACFTLDD
                     GYRDNRDFALPVMREFDAPATVYVASDFAQGSGRLWWIALEMVIARAPAIDVAIGGTA
                     TRLDTATPADKQAAFDRLHDWLRGLPGEHDIEREIAALCARHGVDEMAICRELCMSWD
                     ELKPFADDPLITIGAHTITHCNLARQPETIAAHEMASSRARIESVLQRQVVHLAYPYG
                     DRIAAGPREFALAHASGYKTAVTTRPGMMFPESAGHLTALPRVSLNGNYQDARILPVL
                     TSGAATAMWNGFRRIDAA"
     gene            5056..5862
                     /locus_tag="VN968_24240"
     CDS             5056..5862
                     /locus_tag="VN968_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL32375.1"
                     /translation="MFKDLFSLTGRVALVTGGSRGIGRMIAAGFLSQGAAKVYITARK
                     AGPCEATAKELAAEYGGECIALPIDISTMAGIDMLAGEIRKREPKLDILVNNAGAAWG
                     ADFDEFPESGWDKVMNLNLKTPFFLTKALAAPLRAAASAERPAKVINIASIDGIFVNP
                     METYSYAASKSGLIHLTRRMAVKLIRDHVVVSAIAPGPFKSDMNKAARDNADEVSKRV
                     PSGRIGTDEDMAGAAIYLASRAGDYVVGVTIAVDGGIVYANPGIKGDGWD"
     gene            complement(6088..7524)
                     /gene="boxB"
                     /locus_tag="VN968_24245"
     CDS             complement(6088..7524)
                     /gene="boxB"
                     /locus_tag="VN968_24245"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HXL32376.1"
                     /translation="MNVDYSTKIPNNVNLSEDRQVLKALEGWHPGYMDWWSDMGPEGF
                     QQSLVYLRTAYSVDPRGWAKFDYVTMPEYRWGILLAPQEENRVIPFGENFGKPAWQEV
                     PGEHRATLRRLIVIQGDTEPASVEQQRHLGKTAPSLYDLRNLFQVNVEEGRHLWAMVY
                     LLQKYFGRDGREEADELLRRRSGDADSPRMLGAFNEATPDWLSFFMFTYFTDRDGKMQ
                     LHSLAQSGFDPLSRTCRFMLTEEAHHMFVGETGISRVVQRTCEAMRAAGISDPSDIAK
                     VRALGVVDLPTLQKKLNLHYSLSLDLFGSEVSTNAANAFNAGIKGRYHETQIKDDHRL
                     QNDTYPVLKLVNGEIRRVDEPALTALNMRLRDDYTQDCVKGLLRWNKIISTAGYDFKL
                     TLPNVAFHRQIGEFRDIHATPDGVLIDDATWSKRKGEWLPSADDGDFIASLMTPVTEV
                     GQFAPWISPPKVGIDNKPGDFEYVKIET"
     gene            complement(7648..9336)
                     /gene="boxC"
                     /locus_tag="VN968_24250"
     CDS             complement(7648..9336)
                     /gene="boxC"
                     /locus_tag="VN968_24250"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HXL32377.1"
                     /translation="MAGEDRVLAGGAKHIDFQTDPSRYRHWKLDVDGEVATLTMDVDE
                     NGGLFEGYQLKLNSYDLGVDIELADAVQRLRFEHPGVKVVVVRSAKNRVFCAGANIRM
                     LAGATHAHKVNFCKFTNETRNGLEDSSENSGQRFVTVINGTAAGGGYELALATDHIIL
                     ADDGSAAVALPEVPLLAVLPGTGGLTRVVDKRKVRRDHADYFCTIEEGIKGKRAVQWR
                     LVDEIVPNSKLEAKVAERAKEFAAVSKRNGTGTGMTLTPLTRRFDHNGIRYGLVSVDI
                     DRAARIATISIQAPDAAPPADIDGMIAQGASFWPLQVARELDDAILHLRINELETAML
                     VFKSHGERAGVLACDAFLEANKTHWLVNEIRQYWKRVLKRIDVTSRTLVTLVEPGSCF
                     VGTLAELVFAADRSYMLIGSRQGDNRAPPTIELSAMNFGPYPMSHGLTRLQSRFQADA
                     EDVLRAQNSIGEALDAEQAEALGLVTFAVDDIDWDDEIRVFFEERTSFSPDSLTGMEA
                     NLRFVGPETMESKIFSRLTAWQNWIFQRPNAVGETGALRRYGTGQKAQFDMTRV"
     gene            complement(9471..>9707)
                     /locus_tag="VN968_24255"
     CDS             complement(9471..>9707)
                     /locus_tag="VN968_24255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL32378.1"
                     /translation="LAYIRVPVAIVQGAEDHYGTIRQIEIAQEECYCPVEVTIVPGAG
                     HSPHREAPEAALNAISDFVGRILDADEGSRGQAA"
BASE COUNT         1825 a         3125 c         2985 g         1772 t
ORIGIN      
        1 catcgggccg gctctcttcc cttcggggcc gtatcgtcag ccgccgctat ccccgcgctc
       61 ccgaacatag acggcttcgc gcttcggttt cgcgtccatg cccgccgtcg catggcgcaa
      121 gcgcttgagc cgcgcgccca gtataacctc tcgaagcgtc agcaagatta cagatatgac
      181 gaacggcgca agataataga ggacgcggaa aagaagcatc cccgcgagca ggtcttcccg
      241 gtccatctgc cacaggccga ccagcatggc ggcgtcgaac accccgagcc cgccggggga
      301 atgactggcg aagcccaaaa gcgtcgccga gacaaaaatc accgcgacaa cgacaaagcc
      361 gagattgggt tcgtccggca ccagcacgta catcgccagc gcgcaaaagc cgagatcgac
      421 aatgccgatc gcgatctgca gcagcgtcag cggaccgccc ggcagcacca cggtccacgg
      481 cccgcggccg acctgtctcg gctgggtcca gacccagacc acatatgcca ccagcccgac
      541 aatgatggcg aacgcggcga tacgattcag ccagggcggc agctgatcga tcgcggatgc
      601 cgcttccggg tgataggcga tacccagccc cagcaccgcg gcattgccga gccagaatgt
      661 caggccggcc agaaagcaga ttttggcgac gtcgatcgca ttcaatcccc acgccgaata
      721 gatccggtag cgcaccgcgc ccccggtgaa gacgctggcg ccgacattgt gcccgatcga
      781 ataactgctg aaggccgcga gcgcgttgat gcggtaggga atatcgctgc ggccgatcgc
      841 gcgaaccgcg aacaggtcat agaaagtcag ggtgaagtag cccgccgtca cgaacaaccc
      901 cgccagaacg atctggcgag actcggtctg cttgatggcc tcgatgactt cgttggcatc
      961 gatgccccgc agcatatgat agagcacgta gcacgcgatc ccgatgaccg tgatgctgac
     1021 cacaacaccg aacttgtgca ggatttgctt ctggcgcaga aacgacatcg ccctgcgtat
     1081 tgcttcaagc atctccacct cgaactgcga tcccacgacg acggctgccg tgcgagccct
     1141 gccgccgcct gcgttacgcc tgctctccga cacgatgtcg tagcgcgttt tgagcggcag
     1201 tggaattcgc ttggcgtgaa taaaacgcgc ttcttcaata tattagggag tgttcatgac
     1261 gccggaagca cagttttgaa gcattccctc cggcaacgca aagtccctgg ccgcaacctt
     1321 gcatgcgaag cggtgaagtt ttcgtgatgt caggcggcgg gttttcccga tcgccgctgg
     1381 aaaatcagca gatcgagttc cggcgcttcg cctgtaagac cggttaacaa agcgtggaca
     1441 tgcccacagt ggtgggtctg gtgattgaac cagtgcgcca gtgccggcgc cagttgctgt
     1501 tcgaattctt ccggcgacga gacgcggcgg tatttgatcg tccccgcgat gcggccctca
     1561 tcgagcccgt cgacaaatcc gacaatccgc cggtcctccg cttcgcgcgc ggcgcggagg
     1621 gcgtcgaagg tctcgaacag gatcgcatcc agccggttcg gcgcctcgcc ctcgccggtg
     1681 aaccgctgca tccagatccg gtccgtcacc agcagatgat tcagggtgcc atggaccgat
     1741 ttgaagaagg cgccgcggtc ggcgcggtat tgctcggtcg aaagccgcgc cacagaatcg
     1801 taaagccggc cgttggccca tgcattgtaa tgaccgaaca tccggtaatg ggccgaggga
     1861 gtcatggata ctcaggtctc ctgttggcga ccggatgctc tcaagccgcc ggcgcgtgac
     1921 gcgatagtac cctgtcgcgc agccacgatc ccagcgcgca accggccaga tagcaagcaa
     1981 acatgatgag gccgaggtcg agccaatagc cggggcggcc gggaaccagg cgtgcgagcg
     2041 ccgccgcgac cagtgcggcc accaacaccg cgagccaaag cgacgtccgc ttggatacgc
     2101 ctggaccgcg atgaaccacg gcgatccagc ccatcgcgaa acccagcaac agcgacgcaa
     2161 gcagccagcc ccaatgaaat gtcaccagat atgccattcg ccccctacct caccaaaaaa
     2221 tcgatgcggc ggttttgcgc cttgccttcg tcggtatcgt ttgacgcgac cggctgcgtg
     2281 ctgccatagc ccgtcgcggt gaactggctc gccggcaacc cggctttgac gagataatcc
     2341 gccaccgcct gcgcgcgttt ttcggacagc atctggttga aaccgtcctc gccatcggcg
     2401 tcggtatgtc ccgcaatttc gatgttggcg gccgggcagc gcaacgcggt ttcgaccagg
     2461 cggtcgagca accccaggga atccggatcg atggtggcgc gcccggattc gaaacggatt
     2521 ttccccttgg ccagcagatc cgaaaacagc tgctggcata ccgtggcatc caccggtgcg
     2581 gcctgcggct tgaccgaaat ctcggccttg aactgccagg attgcggaaa atccttgccg
     2641 agaccggccc gcatctgcac cgccgcggca tcgtaaagcg catcgccaga gagctttacc
     2701 tcccggtctg acactaccag agtacctgtc gacaaccggg acagcgcgcc cagcgccggc
     2761 atcaccgcat tggcaaaccc cgacggcgcg cccacgctgg ccttgagatt gtcgaccacc
     2821 ttttcgctga agaatttgcg cccggcggcg gcggcgagcg ccgcatggac attgttatca
     2881 ggcacatagc cggtcagcgt cagcgtgacc gcgaccggat ccttgtaggc ctggaagata
     2941 tagggcggcg ccttgatctc gttggcggcg accgaaaacc cctccgggag ggttttcagg
     3001 gcggccgcga tcgcctcccg gccgcccagt tcgcgcgcca tgccggaaag gctgaccttg
     3061 ccatcggaaa tcgtgatctt gccgtccttc aatttcccga tctgatcgag cagcaacgag
     3121 gccgcatcgt cgaaacgcgg cggcgccccc cgcgacaggt tcatctggtc ggcgacctca
     3181 acgccgccaa gacttgcccg ggccgcctcc agcagcttgg ccttgctggc cggcaacggc
     3241 gcgctgccgc ccaatgtgac cctgacgacg tcccgctcgg ccgaccagac gaacggcctg
     3301 gcttcgggaa cgaggcgggt ttcgtcgtta acgcgccgca cgcccggcac ggcttgcacc
     3361 gacgcagccg ccctgctgcg gccgtcttcc gagaacgcgt ccgccgtcaa cgtgacgtca
     3421 cggccggcaa ccgcaatcct cgtcttgtcg agcacggtat ccttcagcgc ggcggtgctg
     3481 cgcgcggcca gatcggattc cacggttgag gtgttggtcc aggccgcgat agcccaaaag
     3541 atggccagtg gaatcaaacc cggccaccac ttgctgcgcc acctgaaaag tccgtgcatt
     3601 cgacgtctcg cgggtctgga actctgagaa aacaaaccct tgcgaggctg tcaaactgga
     3661 aataacacga aaaaagtccg gtgcaggaca gcctttcgga ctaactgttt cttcagcggt
     3721 ctcccgttag cttcggaatc tcgcccaacc ggccagctac gccagcatga agcagtttcg
     3781 caaccacgtc atccgcgccg gactgggagc gctttacttc agcggggccc atcatctgct
     3841 gcgaccgatc ttcgcagggg tcggcgcgat cttcatgctg caccacgtcc gcccgcgccg
     3901 agacaccgaa ttccagccca atcgtcatct cgaggtcgcg ccggagttcc tgcgcacgat
     3961 gctcgcgcac cttcgcgcgc gtggcgtcga catcgtcacc atggacgagc tccatcagcg
     4021 gttgatcgac ggcaatttct cgcgacgctt tgcctgcttc accctcgacg atggctatcg
     4081 cgataacagg gattttgcgc tgccggtgat gcgggaattt gacgcacctg ccacggtcta
     4141 tgtcgccagc gatttcgcgc aagggtccgg ccggctgtgg tggatcgcgc tcgagatggt
     4201 gatcgccagg gcgcccgcga tcgacgtcgc gatcggaggc actgcgacgc gcctcgatac
     4261 cgctacgccg gccgacaagc aggccgcgtt cgaccggttg cacgactggc tgcgcggatt
     4321 gccgggcgaa catgatatcg agcgcgaaat cgctgcgctg tgcgcccgtc acggcgtcga
     4381 tgaaatggcg atctgccgcg aactgtgcat gtcgtgggat gagttgaagc cgtttgcgga
     4441 cgatcccctg atcacgatcg gcgcacatac gatcacgcat tgcaatctcg ccagacagcc
     4501 tgagacgatc gccgcccatg aaatggcgag cagccgcgca cggatcgaaa gcgtgctgca
     4561 gcgtcaggtg gtccatctcg cctaccccta tggcgacagg atcgccgccg gcccccgcga
     4621 gttcgcactg gcgcacgcaa gcggctataa gaccgcagtg acgacacggc cgggcatgat
     4681 gtttcccgaa agcgctggcc atctgaccgc gctgccgcgg gtttcgctca acggcaacta
     4741 tcaggacgcc cgcatcttgc cggtgctgac ctccggcgcc gccaccgcga tgtggaacgg
     4801 ttttcggcgt atcgacgcgg cgtgatttcg cgcaacgccg cttcttccct tctccctttg
     4861 tgggagaagg tgccttcgat gcgaagcatc gaaggcggat gaggggtaac ggtgtatcga
     4921 taggccggaa cccctcaccc gtcacgaacg cgcttcgcgc gtccgtgcca ccctctcccc
     4981 atccatccgc tggacagcgg cgttccttga ctcatcaaac tcccgcgccc aaaaccatgg
     5041 gcaagggagc aaaacatgtt caaggatctg ttttcactca caggccgcgt cgcgctggtg
     5101 accgggggat cgcgcggcat cggccggatg atcgcggcag gatttctcag ccagggcgcg
     5161 gcgaaagttt acattaccgc gcgcaaggcc ggcccctgcg aggcgaccgc gaaggaactt
     5221 gcggccgaat atggcggcga atgcatcgcg ctgccgatcg acatttcgac catggcgggc
     5281 atcgacatgc tggcgggtga gatcagaaag cgcgagccga agctggatat tctggtcaac
     5341 aatgccggcg cggcgtgggg cgcggatttc gacgagtttc cggagagcgg ctgggacaag
     5401 gtgatgaacc tcaacctcaa gacgccgttc tttctgacca aggcgctggc ggcacccttg
     5461 cgcgccgcgg cgtccgcgga aagacccgcc aaggtcatca acatcgcctc gatcgacggc
     5521 atcttcgtca acccgatgga gacctattcc tacgccgcca gcaaatccgg cctgattcac
     5581 ctgacgcggc ggatggccgt caaactgatc cgcgaccacg tcgtggtgtc ggcgatcgcc
     5641 ccgggaccgt tcaagtccga catgaacaag gccgcgcgcg acaacgccga tgaagtgtcg
     5701 aaacgcgtgc cgtcggggcg catcggcacc gatgaggaca tggcgggtgc tgcgatctat
     5761 ctggcctcgc gcgccggaga ttatgtggtc ggcgtcacca tcgcggtcga cggcgggatt
     5821 gtctacgcga acccggggat caagggagac gggtgggatt aggtttgatc cgtgagattg
     5881 ccacagcgaa ggcgggctcc ctcgccccgc tcttgcgggt agagggttgg agtgaggggc
     5941 cgctgccgac ctgcgcacgc tgacggtggg gtgagcacag tgcctcgccc ctcatccgga
     6001 tcgcaccggg cgatgcctta catcgccgag gcgatccgac ctctccccgc aagcggccgc
     6061 aagcggggcg aggtaagaaa gcgaacctca cgtctcgatc ttcacatact cgaaatcgcc
     6121 cggcttgttg tcgatgccga ccttcggcgg cgaaatccac ggggcgaatt ggccgacctc
     6181 ggtcaccggc gtcatcaggg aagcgatgaa atcgccgtca tcggcggagg gaagccattc
     6241 gccctttcgc ttgctccagg tggcatcgtc gatcagcacg ccgtccggcg tggcatggat
     6301 atccctgaac tcgccgatct ggcgatggaa cgcgacattc ggcagcgtca gcttgaagtc
     6361 gtagccggcc gttgagatga tcttgttcca gcgcaacagg cccttgacgc agtcctgggt
     6421 gtaatcgtcg cgcagccgca tgttgagggc ggtgagcgcc ggctcgtcga cgcgcctgat
     6481 ctcgccgttc accagcttga gcacgggata ggtatcgttc tgcagccggt gatcgtcctt
     6541 gatctgggtc tcgtggtagc ggcccttgat gccggcattg aaggcgttcg ccgcgttggt
     6601 ggaaacctcc gagccgaaca gatcgagcga gagcgaataa tgcagattga gcttcttctg
     6661 gagggtcgga agatcgacga cgccgagcgc gcggaccttt gcgatatcag agggatcgct
     6721 gatccccgca gccctcatcg cctcacaggt tcgctgcacc acgcggctga tgccggtctc
     6781 gccgacgaac atgtgatgcg cctcttcggt cagcatgaag cggcaggtgc gcgacaacgg
     6841 atcgaagccg gactgcgcca gcgagtgcaa ctgcatcttg ccatcgcggt cggtaaaata
     6901 ggtgaacatg aagaacgaca gccagtccgg cgtggcctcg ttgaaggcgc ccagcatgcg
     6961 cggtgagtcc gcatcacccg agcggcggcg caacagttca tccgcctcct cgcgcccgtc
     7021 acgcccaaag tatttctgca gcaggtagac catcgcccag agatggcggc cttcctcgac
     7081 attgacctgg aacaggttgc gcaggtcgta gagcgacggc gcggtcttgc cgaggtgacg
     7141 ctgctgttcg accgatgccg gctcggtgtc gccctggatc acgatcagcc ggcgcagcgt
     7201 ggcgcggtgc tcgcccggta cttcctgcca tgccggcttg ccgaaattct cgccgaacgg
     7261 gatgacgcga ttttcttcct gcggcgccag caagataccc cagcgatact ccggcatcgt
     7321 cacatagtcg aacttggccc agccgcgcgg atcgaccgaa taggcggtgc gcaggtagac
     7381 cagcgattgc tgaaaacctt ccggtcccat gtcgctccac cagtccatgt aaccgggatg
     7441 ccagccttcc agcgccttga gcacctgccg atcctcgctg agattcacgt tgttgggaat
     7501 cttggtgctg taatcgacgt tcatgatgtt catggtggag gtctcctgtg ttggagcgaa
     7561 tgcgttttgt tcggcgaatg gcgagtagcg agtagaaaaa gcatgcgaga acgagcagct
     7621 tactattcgc cattcgctac tcgcccctta aactctcgtc atgtcgaact gcgccttctg
     7681 gccggtgccg tagcggcgca gcgcgccggt ttcgccgacg gcgttgggac gctgaaagat
     7741 ccagttttgc cacgccgtca ggcgcgagaa gattttcgat tccatggtct cgggtccgac
     7801 gaagcgcaaa ttggcttcca tgccggtcag gctgtcgggc gagaagctgg tgcgttcctc
     7861 gaaaaacacc ctgatctcgt cgtcccagtc gatatcgtcg accgcaaagg tgacgaggcc
     7921 gagcgcttcg gcttgctccg cgtcgagcgc ctcgccgatg ctgttctgcg cgcgcaagac
     7981 atcctcggcg tcggcctgaa agcgcgattg cagccgcgtc aggccgtggc tcattggata
     8041 gggtccgaaa ttcatcgccg acagttcgat ggtcggcggc gcgcgattgt cgccctgccg
     8101 cgagccgatc agcatgtagg accggtcggc cgcgaacacg agttcggcga gcgtgccgac
     8161 aaagcacgag cccggctcga ccagcgtcac cagcgtgcgc gaggtgacgt cgatgcgctt
     8221 caacacccgc ttccagtatt gcctgatttc gttgaccagc cagtgcgtct tgttggcttc
     8281 caggaatgcg tcgcaggcca gcacgcccgc gcgctcgcca tgcgacttga acaccagcat
     8341 cgcggtttcg agttcgttga tgcgcaaatg caggatcgcg tcgtcgagtt cgcgtgccac
     8401 ctgcagtggc cagaacgacg cgccttgcgc gatcatgccg tcgatatcgg cgggtggcgc
     8461 agcatcgggc gcctgaatcg aaatcgtcgc gatgcgcgcg gcgcggtcga tatcgacgct
     8521 gacaaggccg taacggatgc cgttgtggtc gaagcggcgc gtcagcgggg tcagcgtcat
     8581 gcccgttccg gtgccgttgc gcttcgagac cgcggcgaat tcttttgcac gttcggcgac
     8641 tttggcctcc agcttgctgt tgggcacgat ctcgtcgacg aggcgccact gcaccgcgcg
     8701 cttgcccttg atgccttcct cgatggtgca gaaataatcg gcgtgatcgc ggcgcacctt
     8761 gcgcttgtcg accacgcggg tcagtccgcc ggtgcccggc aataccgcca gcagcggcac
     8821 ttccggcagc gcgaccgccg ccgagccgtc gtcggcgagg atgatgtgat cggtcgccag
     8881 cgccagttca tagccgccgc cggccgcggt gccgttgatg acggtgacaa aacgctgccc
     8941 ggaattctcc gatgaatcct cgagcccgtt gcgggtctcg ttggtgaatt tgcagaagtt
     9001 gaccttgtgg gcgtgggtcg cccccgccag catgcggatg ttggcgccgg cgcagaacac
     9061 ccggttcttg gcggaccgca ccacgaccac cttcacgccg ggatgttcga agcgcagccg
     9121 ctgcacggcg tcggccagct cgatatcgac gccgaggtcg taggaattca gcttgagctg
     9181 gtaaccttcg aacagcccgc cgttctcgtc gacgtccatg gtcagcgtcg cgacctcgcc
     9241 gtcgacatcc agcttccaat gccggtagcg cgacggatcg gtctgaaaat cgatgtgctt
     9301 cgcgccgccc gcgaggacgc gatcttcacc ggccatgggc cacccttgcg ttcatatgcg
     9361 ttatcttgat atgcacaata atgcatgttt ttgcagcagt ccagtcctga aacgccgaaa
     9421 tcggcatttt gtttcatgtt cggaccggcc gctgagccgc gatcgaggtg ttacgcagcc
     9481 tgtccccgcg agccttcatc cgcatccagg atgcgtccca caaaatccga gatcgcattg
     9541 agcgctgctt ccggcgcttc acgatgcggc gaatgcccgg cgccaggcac gatcgtcacc
     9601 tcgaccgggc agtagcattc ctcctgcgcg atctcgatct ggcggatggt cccgtaatgg
     9661 tcctcggcgc cttgcacgat cgcaaccggc acccggatgt aggcgag
//
LOCUS       DATNOI010000597         2372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_717269,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000597 DATNOI010000000
VERSION     DATNOI010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717269"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VN968_24260"
     CDS             <1..434
                     /locus_tag="VN968_24260"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXL32379.1"
                     /translation="SLIGIGSLHVQHSNGRELRRDVNMIVPIDLLPPILDDLLTYGRP
                     NRPPRPWLGLYATEIEDSIVVAGLSERGPASKTGLKAGDRILAVRDEPVSSLASFWRR
                     VWASGPAGSEVVLQVVRDTETMKVRIPSADRTRLLKGPKLH"
     gene            complement(556..1815)
                     /locus_tag="VN968_24265"
     CDS             complement(556..1815)
                     /locus_tag="VN968_24265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014330335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HXL32380.1"
                     /translation="MLDRLAAVGQSRVRVRTLGGNRAGEMRITRFLRNPRVTPAEMVQ
                     TAAARTAKLVAGRHILAIQDTTSLRDDGDQQSLVLHPTIAIDAQDGALLGLVHAELLR
                     RRGGSKAQRKARPFAQKESRRWLTATEQAGALLAAGAACLTVIADREGDIYEEFAGKP
                     AGVELLIRVGQDRVLADGRRLFACTEGLPELGHLTVALPAAPGRAARQAVLGLRACPV
                     RIARPKRPAAQAAGLPPEVSLTLVEAHEIDPPEGAAAAHWRLLTTHRVTSLAEATLMV
                     GFYRQRWTIEQLFRVMKTKGFDIEAVRIAEDEPFEKLATATLIAALQVLQLVRDRDGT
                     ARRPFADVFDPADQPAMAAICATLEGKTQRQQNPHPSASLAYAAWVCARLGGWTGYYG
                     KPGPMVTLNGLLRFKAMAHGWNLGRLV"
BASE COUNT          387 a          840 c          753 g          392 t
ORIGIN      
        1 gcagcctgat cggcatcggc tcgctgcacg ttcagcattc caacggccgc gagctgcgcc
       61 gcgacgtcaa catgatcgtg ccgatcgatc tgctgccgcc gatcctcgac gatttgctga
      121 cctatggccg gccgaaccgg ccgccgcggc cctggctcgg cctctacgcc accgagatcg
      181 aggattccat cgtcgtcgcc gggttgtcgg agcgcgggcc ggcgagcaag acggggctca
      241 aggccggcga ccgcatcctc gcagtgcgcg acgagccggt ctcgagcctc gcctcgttct
      301 ggcgccgggt gtgggccagc ggcccggccg gcagcgaagt tgtgctgcag gtcgtgcgcg
      361 acaccgaaac gatgaaggtg cgcatcccgt cggccgaccg gacccgcctg ctcaagggcc
      421 ccaaactgca ctgattttcg cgggcgccgt gcgggtgcgc gctctctcaa ccccatggcc
      481 gggcttgacc cggccatcca cgtcttcctt cgatgcagcc gagacgtgga ccctcgggac
      541 aagcccctac tcgattcaca caagtctccc gaggttccat ccgtgggcca ttgctttgaa
      601 tcggagcaag ccgttgaggg tgaccattgg tccgggcttg ccataatagc cggtccagcc
      661 gccgagccgg gcacacaccc aagcggcgta ggcgagcgag gctgagggat ggggattttg
      721 ctgccgctgg gtcttgcctt ccagggtggc gcagatcgcc gccatcgcgg gctggtcggc
      781 ggggtcgaag acatcggcga acggccgccg cgcggttccg tcgcgatcgc gcaccaactg
      841 caggacttgc agggctgcga tcaaggtggc ggtggcgagc ttttcgaacg gctcatcctc
      901 ggcgatgcgc accgcttcga tatcgaagcc tttggttttc atgacccgga acagctgctc
      961 gatggtccaa cgctggcggt agaagccgac catcagcgtg gcctcggcca ggctggtcac
     1021 gcgatgggtc gtcagcaatc gccagtgcgc ggccgctgct ccttcgggcg ggtcgatctc
     1081 gtgcgcttcg accagggtca ggctgacctc tggcggcaga ccggcggcct gcgcggccgg
     1141 ccgcttcggc cgcgcgatcc tgaccgggca ggcgcgcaac cccagcaccg cctgccgcgc
     1201 cgcccgaccc ggcgccgccg gcaacgcgac cgtcaggtgg cccagctcgg gcaggccctc
     1261 ggtgcaggca aacagccggc gaccatcggc cagcacacga tcctgcccga cccggatcag
     1321 cagctccaca ccggccggct tgccggcgaa ctcctcatag atgtcgccct cgcgatcggc
     1381 gatcacggtg aggcaggccg ccccggccgc cagcagcgcg cccgcctgct cggtcgcggt
     1441 cagccaacgc cggctctcct tctgcgcgaa cggccgcgcc ttgcgctgcg ccttcgatcc
     1501 gccgcggcgg cgaagcagct cggcatggac cagccccaac aaggcgccat cctgcgcatc
     1561 aatggcgatc gtcgggtgca acaccaggct ttgctggtcg ccgtcatcgc gcaggctcgt
     1621 cgtgtcctgg atcgccaaaa tgtgccggcc cgccaccaac ttggccgtgc gcgccgccgc
     1681 tgtttgcacc atctcggccg gggttacccg cgggttgcgc agaaaccgcg tgatccgcat
     1741 ctcgccggcg cgattgccac caagcgtgcg cacccgaacc ctcgactgtc ccaccgccgc
     1801 cagccgatcg agcaagaaca gccccctttt tccaggcggc ggtcgccaaa ccgccccagc
     1861 cccattgtca tcgccaccct ccgcgatccc caatgatctg agggattcag aaatggcctc
     1921 gcagcgcaag cccctacacg atgtgtgcat cccgtaggga caagcccggg ggaggggaat
     1981 attttaagcg tttcaatctg attcgatcat gctctagggt caaacccaat aggtcccgcg
     2041 catccccatt agagagctag ggcaagtccg agcctcgcct gagcgccgac gacgctttcg
     2101 cgtcgattcc cgccattccc gtgagcgaaa ttagaccgct ggagtcgacc cgaagcggtc
     2161 ccccaggtcg ggcaagccgt gggctcgtcc tgatccaaag cggacatgaa cgcgcagggg
     2221 gtgactcttt tccgtggccc cgaccgtcct gggtgcagga ggccgatgag cctcactcgt
     2281 cccactctcg ctaaagagtc aaatccgagg agagatcgcg atgctgcttc tcacgggagc
     2341 gaccggtttg gcaggttcct ttatagcgaa cg
//
LOCUS       DATNOI010000598        16706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1122752,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000598 DATNOI010000000
VERSION     DATNOI010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 16706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16706
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122752"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..1506
                     /locus_tag="VN968_24270"
     CDS             319..1506
                     /locus_tag="VN968_24270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HXL32381.1"
                     /translation="MTLTATPRATGSAAWADALLLSFAEAGYVQAEPAILQPAEAFLD
                     LSGEDIRKSLYLTTDPSGEELCLRPDLTIPVARDYLASPRAGQPAGFCYLGPVFRYRG
                     SRPSEFLQAGIESFGRQDRAAADAEMLALALQATRAFGLTEVEIRTGDVALFIALIDA
                     LELYPVWRRRLIKDFNRKISLEQDLERLTLATRSRRNEYEGVLAALAGSDRKAALALV
                     TDLMSIAGTTNVGGRSVSEIADRFLEQSTLKGGALPRDALEVIKRFLAIAGDPGDAVA
                     RLRALADDTKLNITAAIDQFESRVGFMAARGIDTGMTRFSTSFGRGVDYYTGFEFELH
                     AKAQSKSNAVEPLVAGGRYDGLMTQLGAGTPIPAVGFSVWIEALTELGHKPAATSSSR
                     SAS"
     gene            1503..2480
                     /gene="hisG"
                     /locus_tag="VN968_24275"
     CDS             1503..2480
                     /gene="hisG"
                     /locus_tag="VN968_24275"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HXL32382.1"
                     /translation="MTTPFVLAVPSKGRLQENAEAFFTRAGLALAKPRGARDYRGTIN
                     GHDNVEIAYLSASEIASQLARGAVHLGVTGEDLVRESIVDADKRVLLIDNLGFGNANV
                     VVAVPQAWIDVRTMADLDDVTTGFRAQHNRRMRVATKYINLTRTFFASHGVVDYRIVE
                     SAGATEGAPAVGTAEMIVDITTTGATLAANGLKVLDDGVILRSQANLVASKDADWSSE
                     ARETARVILDHIAARARASKYREVRTRFAGCDAALLAEAHNRFGVLSPFGGPTSSGMV
                     TLHCPPARLYALGSFLRAHGAETVSVASLDYVLDRDNPLFAKLEAFLRQ"
     gene            2564..3610
                     /locus_tag="VN968_24280"
     CDS             2564..3610
                     /locus_tag="VN968_24280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXL32383.1"
                     /translation="MMLGTDVSGLSTTAATAAAQGLSIVVPLYNEAAGLAFLHARLGE
                     MAKALRQRCGVACEVVYVDDGSVDATLSIARQLLADALDVQVVSLSRNFGKEAALMAG
                     LDHARRGAILFMDGDGQHPPDIVEKLVGHWIDGGYDVVYTAKAHRDNESLLRRVAVHG
                     FYALINWGARQKIPEDAGDFRLLSPRAAAALRQLPERNRFFKGLASWIGFRQIRVDYE
                     PAAREHGVTTFSPGRLVGLSIEGLTSFSVAPLRFASLLGVLLASAAFLFGLSILWETW
                     MNGKSVPGYPSLVVGLMTIGGVQLIMIGIVGEYIGKILSELKARPIYFVAEHSVKRVE
                     AETGASASERTAAE"
     gene            3607..4452
                     /locus_tag="VN968_24285"
     CDS             3607..4452
                     /locus_tag="VN968_24285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HXL32384.1"
                     /translation="MNGAAPLRRIWLCADDYGLSPGVNRAIRDLIERGRLNATSAMVV
                     GPAIGRDEASLLQAAVANSPRCAIGLHVTLTAPFRPLTMHFQPLYGGMFLRFPKLLRA
                     GLLRRLDPEIIRAELMVQLAAFNELFGRAPDFVDGHQHAQLFPVVRDAFLSAVKEAAP
                     NAWVRQGGRDAPLVRRLGAPKALFLDILSAQFRRRALRANIAFNPGFAGAYDFSRQPD
                     FGVLMRQFLDGLPEGGLIMCHPGFVDETLVNLDPLTVQREHEHAFLGGEHFPDLLAAN
                     KVTLG"
     gene            4578..5372
                     /locus_tag="VN968_24290"
     CDS             4578..5372
                     /locus_tag="VN968_24290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2076 domain-containing protein"
                     /protein_id="HXL32385.1"
                     /translation="MTPQERQLIDDLFDRLSRVEGAPRDPDAVAAIAQGLRTAPNAVY
                     ALVQTVLLQDEALKRANSRIQELEAGGAGEPQQSGGFLDSMRETIFGQNPSRGSVPNV
                     RPPEMASRPVWNSGQVLQQAQGPGHYDQGPYGQPYGAPQAPMGGGMGGGGGSFLGTAA
                     AAAAGVVGGSLLLGSIRSMMGGGHQSFGDSNAFGDKATSQTPWSDQSNSELARDAGIN
                     DIGSSGNRTDDNSRAGLFDSASNDDDHDDMDVDSDDFGGDGDSDYA"
     gene            complement(5458..6261)
                     /locus_tag="VN968_24295"
     CDS             complement(5458..6261)
                     /locus_tag="VN968_24295"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXL32386.1"
                     /translation="MFKRMHLALLAGASCFCAGLHQAAAIEAAPLLEPTVIYAEQPAQ
                     RPLAMPTAYAGRSNMGGGFIEFLFGDGQSQGERYQQQPGYQQQPSYQPQRSLFPQFEP
                     QQRLRRQEDAGEPEQGAFDPKFERQLVDYHGREGAGTIVIDTPNKFLFLVQGDGKALR
                     YGIGVGRPGFTWSGVKQISAKKEWPAWTPPPEMLARRPDLPRHMEGGPQNPLGARAMY
                     LGSSLYRIHGSNEPWTIGTNVSSGCIRMRNEDVIDLYGRVNVGARVVVI"
     gene            6555..7325
                     /locus_tag="VN968_24300"
     CDS             6555..7325
                     /locus_tag="VN968_24300"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HXL32387.1"
                     /translation="MPVHRRRFRIEQAVVGDMPMPAAADGDVGPIHHEIMAELRAIRA
                     QMASPGRSVTTETIGATVNREVAEALSLLETYRAQIEQCEKLKVELDLIHDAITRTKR
                     EIATLHGKSFNGEEMAKVNGELGAVVGGTEEATQQILEAAEAIDNAAAALSKVNTLDQ
                     QKLLSEEIQERVVSIFEACNFQDLTGQRISKVMTTMKFIENHIVVMMDIWGGVDAIKA
                     HAPAHIDEREGDDRLLNGPKLDGDAGHASQNDIDALFD"
     gene            complement(7523..8035)
                     /locus_tag="VN968_24305"
     CDS             complement(7523..8035)
                     /locus_tag="VN968_24305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32388.1"
                     /translation="MVRSCNARAVANLGIASALTLFLGACGGISLPSLSSSSAAPEPG
                     VEPEMPASIRADEIVGRWGLASFQNPNDRARTEVQAKAQCKQPYVIGAGTAGGVVMHL
                     ADQATPQELRLKGTSSGKNYIGPAGPAGGEQDREIVSFDGRVLVTRFIDKDAATRYGN
                     MVYVRCGARA"
     gene            8366..8662
                     /locus_tag="VN968_24310"
     CDS             8366..8662
                     /locus_tag="VN968_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXL32389.1"
                     /translation="MAKSQFRPLHDRVVVKRIDAEQKTKGGIIIPDSAKEKPSQGEIT
                     AIGPGGRDESGKLIPIDLKVGDRVLFGKWSGTEIKLDGEELLIMKESDIMGVLA"
     gene            8847..10496
                     /gene="groL"
                     /locus_tag="VN968_24315"
     CDS             8847..10496
                     /gene="groL"
                     /locus_tag="VN968_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475125.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXL32390.1"
                     /translation="MAAKDVKFSGDARERMLRGVDILANAVKVTLGPKGRNVVIEKSF
                     GAPRITKDGVTVAKEIELEDKFENMGAQMLREVASKTNDTAGDGTTTATVLAQAIVRE
                     GAKSVAAGMNPMDLRRGIEIAVAAVVKDLEKRAKPVASSSEIAQVGTISSNGDTAIGK
                     MIAQAMQKVGNEGVITVEENKSLETEVDIVEGMKFDRGYLSPYFITNAEKMTAELEDV
                     YVLLHEKKLSGLQAMLPVLEAVVQSGKPLLIVAEDVEGEALATLVVNRLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAILTGGQLISDELGMKLESVTINMLGRARKVVIDKENTTIVS
                     GAGKKKDIEARVGQLKAQIEETTSDYDREKLQERLAKLAGGVAIIRVGGATEIEVKEK
                     KDRVEDALNATRAAVQEGIVPGGGVALLRTKKAVGRIHNDNSDVQAGINIVLKALEAP
                     VRQIAENAGVEGSIVVGKILENKTETFGFDAQIEEYVDMVAKGIIDPAKVVRTALQDA
                     ASVAALLVTTEAMVAELPKEAAPPMHGGGGGGMGGMGGMGF"
     gene            10794..11990
                     /locus_tag="VN968_24320"
     CDS             10794..11990
                     /locus_tag="VN968_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32391.1"
                     /translation="MRALLLVLIGLMTASSDLALAQIKPSPKAAPGANEVRYFTSIDG
                     LMAGNADVILKETRQGKSVTAAVLDVCYPAEKGSDRKDRFVVNLAVSGQTLTGTTQSL
                     GDKLPVTVKLTRRPTGDTFEFRGQIGIGQTVTEVTSTDNSDLSEKEFQDNQATDDGIA
                     AAPKDFTEASPEAVGVKVKLEAALDFLKGLKGQDAEVSLSSLAVSCEALRAGEQTINI
                     SVDPDRAAALIAKSKSLPGVVAAGWTSGVVEMDRSIRFAAADWRDGDKLNKNKLAAAI
                     ANVLSKTLAAKSAAATWSASTGKLKLTFKRPSQLFPALGLTETIEILALVAPDKPGTT
                     DRLMLWIGNPVISTTDETAGAKLNLAEEASADEEGDAKDDNGSVDALAKEFKAQRWDA
                     DKSAWK"
     gene            12179..13759
                     /locus_tag="VN968_24325"
     CDS             12179..13759
                     /locus_tag="VN968_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL32392.1"
                     /translation="MARYDVVVIGAGLGGLTAGAILARAGRKVLVIERSNSVGGAASS
                     YESGDLLVEGSLHETSDPHDPRDPKHHALRRAGVIDAVKWIPSGTFYQARGGPLGQPF
                     VLPDNFDAARRALIERFPEARAGIDQLLEEMERIASAMGALSQGSRAFRKPREALGAL
                     LDLLSGFRDWRLPLSQKLDRVFGDNEAIKCALAANLSYYHDDPATLWWVFFAIAQGGY
                     LLTGGRYVQGGPQRLSSALARAIKLAGGEVLMRRAVSRIAPGLQGHSSTVTHTARDGS
                     DAQAVECVRIVANAAPAALAPLMPATAAEKLTANDRRHMPSISLFALTLGLSRPPREF
                     GISAYSTQLLPPEMARLSDYAQGTALMAEEPGERMPPMSIVDYAAIDSGVPAPPYVLL
                     ICGPDRLSNWDSSDMDAYREKRGRWQQAIVGYLSSVYPGLADAVVASSFNTALSVRQD
                     LNAPDGAVYGFAPTPPRSIWRLPVRSPRTAVPGLYLASAYAGFGGYTGVVHSAGACAD
                     MILRESLSPRFDRIETGL"
     gene            complement(13855..14157)
                     /locus_tag="VN968_24330"
     CDS             complement(13855..14157)
                     /locus_tag="VN968_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HXL32393.1"
                     /translation="MTLDAARQPGSKRIPTLDIGDRGEATLDLWCKGGKGQFSVAGNT
                     VIFVAGPLEDRACPPARAQADDELVAALSEAATWKRQGDLVSFMGTKSLRFRINTN"
     gene            complement(14563..16227)
                     /locus_tag="VN968_24335"
     CDS             complement(14563..16227)
                     /locus_tag="VN968_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HXL32394.1"
                     /translation="MWNQIYNPLGNATLSTIAAAIPVVTLLVLIASGKVKAHIAAIIA
                     VILTNLIAIFIFTMPAGMSIRASLLGIVAGFFPIGWIVLNVIFLYQLTVATGQFDLLK
                     RAVGGVTEDRRLQLLLIAFSFGAFFEGASGFGTPVAVTGAVLIGLGFSPLAASGLSLI
                     ANTAPVAYGALGTPIAGLAQVTGLDPYILGAMVGRQLPVFSLIVPFWVVWAFAGWKGM
                     KDVWPAILVTGVSFAVPQFVISNYINPWIVDIGASLISMGCLILFLKVWQPKVLWLSP
                     ALRGKDESIATMKPAIPLDKTALTQGELFSALLPWIIVCIVMLIWGNGAFKTWANSIF
                     FYKYPIPELHNMINTMPPVAAKPTPQGASFDFFYLSFTGTGMLIAAIIAGFLAGFSPA
                     RMIAVYGRTIRLCAISLITISAMLAIGTLTRLSGVDATLGLAFAATGVLYPFFGTLLG
                     WLGVALTGSDTASNILFGNLQKITSEQLGLSSVLMGAANSSGGVMGKMIDAQSIVVAS
                     TATNWYGHESTILRFVFKHSIALACLVGLLVMLQAYVYPFTEMVLK"
BASE COUNT         3280 a         5220 c         5190 g         3016 t
ORIGIN      
        1 cacgaagtcc tgcacattcg gcggcagcag cagcgtctga tcgatattcc agggacgaaa
       61 atacttgctc atgggcccaa ggttgaacca gactcgctca gagttgaaca gccactattc
      121 ggacaggctc ctagccgttc attaagccac ttggattagg ggactttgga ttaaggcact
      181 tgagattaag gcacttggga ttaaggcact tggattaagg cacttggccg atcctggtcg
      241 aaaccccgcc cgggccgtgc taaggggccg taccggctga cgctttgcaa cctgtttttc
      301 ggatatttgg acgtcgagat gaccctgacc gccacccctc gcgcgaccgg atccgccgca
      361 tgggcggacg cgctgctgtt gtcgtttgcc gaggccggct acgtccaggc cgagcccgca
      421 atcctgcagc ccgccgaggc attcctcgac ctctccggcg aggacatccg aaaaagcctc
      481 tatctgacca ccgatcccag cggtgaggag ctgtgccttc gtcccgatct caccattccg
      541 gtggcacggg attatctcgc ctccccccgc gctggccagc cggcggggtt ttgctatctc
      601 ggcccagtat ttagataccg tggcagccgg ccgagtgaat tcctgcaggc cggcatcgaa
      661 tccttcggcc ggcaggatcg cgccgcagcc gacgccgaaa tgctcgcgct ggccctgcag
      721 gcaacgcgcg cattcgggct gaccgaggtc gaaattcgca ccggcgacgt ggcgctgttc
      781 attgcgctga tcgatgccct cgagctctac ccggtgtggc ggcggcggct gatcaaggat
      841 ttcaaccgca agatcagtct ggagcaggat cttgaacggc tcacgctcgc gacccgatcg
      901 cgccgcaacg aatatgaggg tgtgctggct gcgcttgcgg gttccgaccg caaggcggcg
      961 ctggcgctgg tgaccgatct gatgtcgatc gccggcacca ccaatgtcgg cggccgttcg
     1021 gtctcggaaa tcgccgatcg cttcctcgag cagtcgacct tgaagggcgg cgccttgccg
     1081 cgcgatgcgc tcgaggtgat caagcgattc ctggccatcg ccggcgaccc cggcgacgcg
     1141 gtcgcgcggt tgcgcgcgct ggccgacgac accaaactca acatcaccgc cgcgatcgac
     1201 cagttcgaaa gccgcgtcgg cttcatggcc gcgcgcggca tcgacaccgg catgacacgg
     1261 ttttccacct cgttcgggcg cggcgtcgat tattataccg gcttcgaatt cgagctgcat
     1321 gccaaggcac agtccaagag caatgccgtc gagccgctgg tggcgggcgg acgctatgac
     1381 ggactgatga cccagcttgg ggccggcacg ccgatcccgg cggtcgggtt ctcggtctgg
     1441 atcgaagcct tgacggaact gggccacaag cccgccgcaa ccagctcaag ccggagcgcg
     1501 tcatgacgac gccattcgtt ctcgcggttc cgtcgaaggg tcggctgcag gaaaacgccg
     1561 aggcgttctt cacgcgtgcc ggacttgcgc tggcaaaacc gcgtggcgcc cgcgactatc
     1621 gcggcaccat caacggccac gataatgtcg agatcgccta tctctcggca agcgagattg
     1681 cctcgcagct tgcgcgcggc gcggtgcatc tcggcgtcac cggcgaggat ctggtgcgcg
     1741 aaagcatcgt cgatgccgac aagcgcgtct tgttgatcga caacctcggc tttggcaacg
     1801 ccaatgtcgt ggttgcggta ccgcaggcct ggatcgacgt ccgtaccatg gccgatctcg
     1861 acgacgtcac caccggtttc cgtgcccagc ataaccggcg gatgcgggtt gcgaccaaat
     1921 acatcaatct cacccggaca ttttttgcgt ctcacggcgt cgtcgactac cggatcgtcg
     1981 agagcgccgg cgcgacggaa ggcgccccgg cggtcgggac cgccgaaatg atcgtcgaca
     2041 tcaccacaac aggcgcgacg cttgccgcca acggtctcaa ggtgcttgac gacggcgtga
     2101 tcctgcgcag ccaggccaac ctcgtggcat cgaaggatgc cgactggtcg agcgaggccc
     2161 gcgagaccgc gcgcgtgatc ctggatcata ttgcggcccg cgcccgcgcc agcaaatacc
     2221 gcgaagtccg tacccgtttt gccgggtgcg acgcagcatt gcttgccgag gcgcataatc
     2281 gcttcggcgt gctctcgccg ttcggcggcc cgacatcttc gggaatggtg acgctgcact
     2341 gtccgccggc gcggctttac gcgctcggca gctttcttcg cgcgcacggc gcagagacgg
     2401 tgtcggtggc gtcgctcgac tacgtgctcg atcgtgacaa cccgctgttc gccaagcttg
     2461 aggcattcct gcggcagtga gacggagggt gttttcgttc atcgttgcga cagctagcaa
     2521 cgagtaacat atgcggtcat atgggcggct tggaacctga tcgatgatgc tgggtacgga
     2581 tgtttctggc ttatcaacca ctgcggccac ggctgcggcg caggggctgt cgatcgtcgt
     2641 gcccttgtat aacgaggcgg cggggctggc gtttctgcac gcgcggcttg gcgaaatggc
     2701 caaagcgctc cggcagcgct gtggcgtggc gtgcgaggtg gtctatgtcg atgacggcag
     2761 cgtcgacgcg acgctgagta ttgcccgtca actcctggcc gatgcgcttg acgtccaggt
     2821 cgtgtcgctg tcgcgcaact tcggcaagga agccgcgttg atggccggac tggaccatgc
     2881 ccggcgcggc gcgatcctgt tcatggatgg cgacggtcag catccgcctg acatcgtcga
     2941 gaaacttgtc ggccactgga tcgatggcgg ctatgacgtg gtctataccg ccaaggcgca
     3001 tcgcgacaac gaatcgttgc tgcggcgcgt ggcggtgcac ggtttctacg cgctgatcaa
     3061 ttggggcgcg cggcagaaaa tccccgaaga tgccggcgac ttccgcctgc tgtcgccgcg
     3121 cgccgcggcg gcgctgcggc agcttcccga gcgcaatcgc ttcttcaagg ggctggcgag
     3181 ctggatcggt ttccgccaga tccgtgtcga ctacgaaccc gcagcccgcg agcacggcgt
     3241 caccacgttc agccccggcc ggctggtcgg actttcgatc gaaggcctca cctcgttctc
     3301 ggtcgcgccg ctgcgttttg ccagcctgct cggcgtcctg ctcgcttcgg cggctttctt
     3361 gttcggcctt tcgatcctct gggaaacctg gatgaacgga aaatcggttc ccggctatcc
     3421 ctcgctggtg gtcggcctta tgacgatcgg cggcgtgcag ctcatcatga tcggcattgt
     3481 cggagaatat atcggcaaga ttctttcgga gttgaaagcc cggccgatct atttcgtagc
     3541 tgaacacagt gtcaaacgcg tcgaggcgga aacaggcgcg agcgccagcg aaaggaccgc
     3601 cgccgaatga acggcgccgc gccgctgcgc cgcatctggt tatgcgccga cgattacggt
     3661 ctcagtcccg gcgtcaaccg cgccattcgc gacctgatcg agcgtggccg tctcaatgcc
     3721 acatcggcga tggtggtcgg cccggccatc ggccgtgacg aagccagctt gctgcaagcc
     3781 gccgtcgcca acagtccccg ttgtgcgatc ggcctgcacg tcacgttgac ggcgccgttt
     3841 cgtcccctga cgatgcattt tcagccgctt tacggcggca tgttcttgcg ctttccaaaa
     3901 ctgctgcgcg cgggcctgct gcggcggctc gatcccgaaa tcatccgtgc ggaactgatg
     3961 gtgcagctcg cggcgttcaa cgaactattt ggccgcgccc cggatttcgt cgacggtcac
     4021 cagcatgcgc aactgtttcc ggtggtgcgc gacgcgttcc tgtcggcggt gaaagaagcc
     4081 gcgcccaacg cctgggttcg ccagggcgga cgggatgcgc ctttggtccg gcggctcggc
     4141 gcgcccaagg cgttatttct cgacatcttg agcgcgcaat ttcgccgccg tgccttgcgc
     4201 gccaatatcg cattcaatcc gggattcgcc ggcgcttatg atttttctcg acagcccgat
     4261 tttggcgtct tgatgcgaca gtttctcgat gggctaccgg agggcggcct catcatgtgt
     4321 cacccggggt tcgtcgacga gaccctggtc aacctcgacc cgctgacggt ccagcgcgaa
     4381 cacgaacacg ctttcctcgg cggcgagcat ttcccggacc tcctggctgc gaacaaggtt
     4441 acattgggtt gatgtcggct ggaaaaccgg cccgccactt tgtcgcatac cgaaatttaa
     4501 tccggggcag cactactggc gacataaagc ccccctacat ctgcaccgcg cttcgaggcg
     4561 agcgagggag agaatccatg acaccgcaag aacgccaatt gattgacgat cttttcgacc
     4621 ggctctccag ggtggagggc gcaccgcgcg atccggatgc cgtcgccgct attgcccagg
     4681 gtttgcgcac agcgcccaat gccgtttacg cgctggtaca aacggtcctg ctccaggacg
     4741 aagcgctgaa gcgcgccaac agccgcatcc aggaactgga ggctggcggc gcgggcgaac
     4801 cacaacaatc cggcggattc ctcgattcga tgcgcgaaac gatattcggg cagaatccat
     4861 cccgcggctc ggtgccgaac gtccgcccgc ccgagatggc gagccgtccg gtctggaaca
     4921 gcggccaggt gctgcagcag gcgcaaggtc ccggacacta tgatcaaggg ccctacggtc
     4981 agccctatgg cgcgccccag gcacccatgg gcgggggcat gggcggaggt ggcggctcgt
     5041 ttctcggaac cgcagcggct gcggcggccg gtgtggtcgg cggatcgctg ctgctcggca
     5101 gcattcgctc gatgatggga ggcggccatc agagcttcgg cgatagcaac gcctttggcg
     5161 acaaggccac cagccaaact ccgtggagcg atcaatccaa cagcgaattg gcgcgcgacg
     5221 ccggcatcaa cgacatcggt tcatccggca atcgcaccga tgacaattcg cgcgcagggt
     5281 tgttcgattc tgcctcgaac gatgacgatc atgacgacat ggatgttgat tccgacgact
     5341 tcggcggcga cggggacagc gactacgcct gatcaattct tgctggcgca acgacaaaag
     5401 cggccgcccg gatcgggcgg ccgcttttgg ttttcaggcc gccagaaatc agacccgtca
     5461 gatgacgacg acccgcgcgc cgacattgac ccggccatac aggtcgatca cgtcctcatt
     5521 acgcatccgg atgcagcctg atgacacgtt ggtgccgatg gtccagggct cgttggaacc
     5581 gtggatgcga tagagcgacg atcccagata catcgcgcgt gcgccgagcg gattttgcgg
     5641 gccgccttcc atgtgccgcg gcagatcggg ccgccgcgcc agcatttccg gcggcggcgt
     5701 ccacgccggc cattccttct tcgccgaaat ctgcttgacg cccgaccagg tgaagcctgg
     5761 gcggccgacg ccgatgccgt agcgcagcgc cttgccgtcg ccctgaacca ggaacagaaa
     5821 cttgttgggc gtgtcgatca cgatcgtgcc ggcaccttcc ctgccgtgat aatcaaccag
     5881 ttgcctttcg aatttcggat cgaacgcccc ctgctcgggt tcgcccgcat cttcctgccg
     5941 gcgcaaccgt tgctgcggct cgaattgcgg gaacagcgaa cgctgcggtt gataggatgg
     6001 ctgttgttga tagccgggct gctgctgata gcgctcacct tgtgactgtc cgtcgccgaa
     6061 caggaactcg atgaaaccgc cgcccatatt ggagcgccct gcataagccg ttggcatcgc
     6121 cagcggccgc tgcgcgggtt gctcggcgta aatcacggtg ggttccagaa gtggcgcggc
     6181 ctcgatcgct gcggcttgat gaagtccggc acaaaaacaa gatgctccag cgaggagcgc
     6241 aagatgcatt ctcttgaaca tcgacgtact ctgtactgtt tcgtcatggc gagcgcccgg
     6301 tcggcgccct tgcacgccac cagttagaat taaaaccgca tcggtttggt aaacagaaac
     6361 ggcctttgga ttcaccacat cgccaattcc gcgcggtttc atccgccagc gtttattttc
     6421 ggtgaacgcg ggagcatatg gttaatcgcg ggtgtgcgaa tacaagccaa ctcgcgagaa
     6481 gattcttccg gcgtgaaccc cgtgttaaat cgccttcatg taaaacgtga cgagtgcagg
     6541 ttaggaaaaa tctcatgccg gttcatcgca gacgttttcg tatcgaacaa gcagtcgtcg
     6601 gcgacatgcc gatgccggca gccgctgacg gcgatgtcgg tcccattcat cacgagatca
     6661 tggccgagct tcgcgccatc cgcgcccaga tggccagccc cggcaggagc gtgacaaccg
     6721 agaccatcgg cgcgacggtc aatcgcgagg tcgccgaagc gctttcgctg ctggaaacct
     6781 accgggccca gatcgagcag tgcgagaagc tcaaggtcga gcttgacctt attcacgatg
     6841 ccatcacccg caccaagcgg gaaatcgcca cccttcacgg caagagcttc aacggcgagg
     6901 aaatggccaa agtcaacggc gagctcggtg ccgtggtcgg cggtaccgaa gaagccacgc
     6961 agcaaattct ggaagccgcg gaggcgatcg ataatgcggc ggccgcgctt tcgaaggtga
     7021 acaccctgga tcagcaaaag ctgctcagcg aggaaatcca ggaacgtgtc gtttccatct
     7081 tcgaggcctg caatttccag gatctgaccg gtcagcgcat cagcaaggtg atgaccacga
     7141 tgaaattcat cgaaaaccat atcgtggtga tgatggatat ctggggtggc gtcgatgcga
     7201 tcaaggcaca cgcgcctgcc catatcgacg aacgggaagg cgacgaccgg ttgctgaacg
     7261 gtccaaagct ggacggcgac gccggtcatg cctcgcagaa cgacatcgac gccttgtttg
     7321 actgagcctt gatggatcct tgcgccgggg tggcggcacc ctgcggccgg agcgccagcg
     7381 tcggattcaa ctcccgttga atcagactct ggcgctatct ttttggcatc atcgttccgg
     7441 aaaaccggat tccgccgcgc tggagaaata ccctgggcgt caggccgcag atttcagcgc
     7501 gggccgccgg attggcgatg gatcaggcgc gggccccgca acggacatag accatgttgc
     7561 cataccgggt ggcggcatcc ttgtcgatga agcgggtgac cagaacgcga ccgtcgaagg
     7621 aaacaatctc gcgatcctgc tcgccgccgg ccggtccggc cgggccgata taattcttgc
     7681 cgctcgaggt gcccttgagc cgcagttcct gcggcgtcgc ctggtcagcc agatgcatga
     7741 ccacgccgcc cgcggtgccg gcgccgatga cataaggctg cttgcactgc gccttggcct
     7801 gaacttcggt gcgtgcgcgg tcattgggat tctggaacga tgcgagaccc cagcgcccga
     7861 caatctcgtc ggcgcggatc gacgccggca tctcgggctc cacgccgggc tcgggagccg
     7921 ccgaagagga tgacaatgac ggcaggctta tgcctccgca cgcccccagg aataacgtta
     7981 gcgccgaggc gatccccaga ttggcaaccg cacgcgcgtt acatgacctg accatgtttt
     8041 cccctcaagc aaattcgtgg ccgcacccgt cccgcagcca agcgtaaaac ctcagtcgga
     8101 gcaatgatgt ccagtcaatt acgctggcgc tccgatatag tttccgtgaa agcatcctat
     8161 attggtctca ccagaggctt aaccaccttt gcttgacagg atcggcgtca agccatattc
     8221 ccgggcgcac tattagcact ccgagagcgt gattgctaag gatgcgagct tgttttgccc
     8281 cttggcggtg tcgaatacgc ccgtattccg atccactttc gatcgcagaa cagaccctga
     8341 gaaccgagcc tccaaaggaa ccgtcatggc taagtcccaa tttcgtcccc tgcatgaccg
     8401 tgtcgtggtc aaacgtatcg acgccgaaca aaagaccaag ggcggcatca tcattccgga
     8461 tagcgccaag gagaagccct cgcagggcga gatcacggcg atcggtccgg gcggccgaga
     8521 cgaatccggc aagttgatcc cgatcgacct caaggtcggc gaccgtgtgc tgttcggcaa
     8581 gtggtcgggc accgagatca agcttgacgg tgaggaactt ctgatcatga aggaatccga
     8641 catcatgggc gtgctggcct gagaccagcc catcctgaga ccaacccaca cagccgctac
     8701 acactactcg tcatgcccgg gcttgatccg ggcatccatc ctcttcggaa gagtcttgcg
     8761 aaagtgatgg attgccgggt caaacccggc aatgaaggta agggagcgcg gcaacgaccg
     8821 tatccaatca tccaggaggg aatccaatgg ctgccaagga cgttaaattt tccggagacg
     8881 cgcgtgaacg catgctgcgc ggcgtcgata ttctcgccaa tgcggtgaag gtgacgctgg
     8941 gtccgaaggg tcgcaatgtc gtcatcgaaa agagctttgg cgcgccgcgc attaccaagg
     9001 acggcgtcac cgtcgccaag gaaatcgaac tggaggacaa gttcgagaat atgggcgcgc
     9061 agatgctgcg cgaagtcgcc tccaagacca acgacaccgc cggcgacggc accaccaccg
     9121 ccaccgtgtt ggcccaggcg atcgtgcggg aaggcgccaa gtcggtcgcc gccggcatga
     9181 acccgatgga cttgaggcgg ggcatcgaaa tcgcggtggc ggccgtcgtc aaggatctcg
     9241 agaagcgcgc caagccggtc gcttcatcat ccgaaatcgc ccaggtcggc accatctcct
     9301 ccaatggcga tacagcgatc ggcaagatga tcgcgcaggc gatgcagaag gtcggcaatg
     9361 agggcgtcat caccgtcgag gagaacaaat cgctcgaaac cgaagtcgac attgtcgagg
     9421 gtatgaagtt cgaccgtggt tacctgtcgc cctacttcat caccaacgcc gagaaaatga
     9481 ccgcggagct tgaggatgtc tacgtgctgc tgcacgagaa aaagctgtcc ggcctgcagg
     9541 cgatgctgcc ggtcctggaa gcggtggttc aatccggcaa gccactcttg atcgtcgccg
     9601 aggacgtcga gggcgaggcg ctggccacgc tggtcgtcaa tcgcctgcgc ggcggcctga
     9661 aggtcgccgc cgtcaaggcg cccggcttcg gcgaccgccg caaggcgatg ctggaagaca
     9721 tcgcgatcct gaccggcggt cagctgatct cggacgaact cggcatgaag ctcgaaagcg
     9781 tcaccatcaa catgctcggc cgcgcccgca aggtcgtgat cgacaaggaa aacaccacca
     9841 tcgtcagcgg tgccggcaag aagaaggata tcgaagcccg ggtcggccag ctcaaggcgc
     9901 agatcgagga gaccacctcg gactacgacc gtgagaagct gcaggagcgc ctggccaagc
     9961 tcgccggcgg cgtcgcgatc attcgcgtcg gcggcgccac cgagatcgag gtgaaagaga
    10021 aaaaggaccg cgtcgaggac gccctcaacg ccacccgcgc cgccgtgcag gaaggcatcg
    10081 tgccgggcgg cggtgtcgcg ctgctgcgca ccaaaaaggc cgtcggccgt atccataacg
    10141 acaattccga cgtccaggcc ggtatcaata tcgtgctgaa agcgctggaa gcgccggtcc
    10201 gccagatcgc cgagaacgcc ggtgtcgagg gctcgatcgt cgtcggcaag atcctcgaga
    10261 acaagaccga gactttcggc ttcgatgcgc agatcgaaga gtatgtcgac atggtcgcca
    10321 aaggcatcat cgatccggcc aaggtggtcc gcaccgcgct gcaggatgcc gcctcggtag
    10381 ccgccctctt ggtgacgacc gaagccatgg ttgccgaact gccgaaggaa gcagcacccc
    10441 cgatgcacgg cggcggtggc ggcggcatgg gtggaatggg cggcatgggc ttctagcgag
    10501 cgcatgatcc ggaaaagcat gccctcgggc tttgacccga gggtggttac cggttttccg
    10561 aaaagatcat gctcaaacaa aaagatagag cgggatgacg attcgaagaa aactcatccc
    10621 actcctagcg accggcccgg tgccgacgtc agcgatcgac caaggccgcc tccgggcggc
    10681 cttctttttt ggcgaatagt gcagaggttt gctagtgaag gccggcctgg ttccgattcg
    10741 accggaatcg aaagcgcttt tcgacaccgt tggtatcttt cggggaattt caaatgcgcg
    10801 cgcttctgct tgtcctgatc ggcctgatga cagcctcctc ggatctcgct cttgcccaga
    10861 tcaagccctc gcccaaggcc gcgccgggtg caaacgaagt caggtatttc acgtcgatcg
    10921 atgggctgat ggctggcaat gccgacgtca tcctcaagga gacgcggcag ggcaagagcg
    10981 tcaccgcggc ggtgctcgac gtctgctatc cggccgagaa gggctccgat cgcaaggacc
    11041 gctttgtcgt caacctcgcc gtcagcggcc aaaccttgac cggcaccacg cagagcctcg
    11101 gcgacaaatt gcccgtgacg gtgaagctta cgcgccggcc gaccggcgac accttcgaat
    11161 tcaggggcca gatcggcatc ggccagaccg tgacggaggt gacctcgacg gacaattccg
    11221 atctcagtga aaaggaattc caggacaacc aggcaaccga tgacggcatc gcggcagcgc
    11281 ccaaggattt taccgaagct tccccggaag cggttggcgt gaaggtaaag ctggaggcag
    11341 cgctggattt cctgaaggga ttgaagggcc aggacgcgga agtttccttg agcagcctcg
    11401 ccgtttcatg cgaggcgctg cgcgccggcg agcagacgat caacatcagc gtcgatccgg
    11461 atcgcgccgc ggcgctgatc gcaaagtcga aatcgttgcc cggcgtggtc gccgccggct
    11521 ggaccagcgg tgtcgtggaa atggatcgct cgatccgttt tgcggccgcc gactggcgcg
    11581 acggcgacaa gctcaacaag aacaagctcg ccgcggcgat cgccaacgtc ctgtcaaaaa
    11641 cgctcgcggc gaagtcggcg gcggcgacat ggagcgccag caccggcaag ctgaaattga
    11701 cgttcaaacg gccgagccag ctattccccg cgctcggcct caccgagacg atcgagattc
    11761 tcgctctggt ggcgccggac aagcccggca cgaccgaccg gctgatgctg tggatcggca
    11821 atcccgtcat ttcgacgacg gacgaaaccg ccggcgcgaa actcaatctg gccgaggagg
    11881 ccagcgccga cgaggaaggc gatgcgaagg acgacaatgg ctcggtcgat gcgctggcga
    11941 aggaattcaa ggcccagcgc tgggatgcgg ataaatcggc ctggaaataa cggagcttcc
    12001 aataatttgc gcgtattctg atcgcaaaac cgggtatccg cgtttgcgga acacgcgcta
    12061 tcgatccgaa ggacagcccc gcacgcaccc gctccatgca tgggcgcatt tgacacgccg
    12121 caccatagcg gcaatgaaca aggcttatgg ggcaagggct ttcgggattt cacgctgaat
    12181 ggcgcgctat gacgtagtgg tgatcggcgc cggtcttggt ggcttgaccg ccggcgcgat
    12241 cctggcgcgc gcgggacgca aggtcctcgt catcgagcgc agcaactcgg ttggcggcgc
    12301 ggcgtcgagc tacgaatccg gcgacctgtt ggtcgaaggc tcgctgcacg aaaccagcga
    12361 cccgcacgat ccgcgcgatc cgaagcacca cgcgctgagg cgtgcgggtg tcatcgacgc
    12421 cgtaaaatgg atcccaagtg gcacattcta ccaagcgcgc ggcggcccgc ttggccagcc
    12481 gtttgtgctg ccggacaatt tcgacgcggc gcggcgcgcc ctgatcgagc gcttcccgga
    12541 ggcccgcgca gggatcgatc aactgctgga agaaatggag cgcatcgcat ccgcgatggg
    12601 cgcgctttcg caaggcagcc gcgccttcag aaagccgcgc gaagctctgg gcgccctgtt
    12661 ggacttgtta tccgggttcc gcgactggcg cctgccgctg tcgcaaaagc tggaccgggt
    12721 cttcggcgac aacgaagcca tcaaatgcgc gctggccgcc aatctctcct attatcacga
    12781 cgaccccgcg accctgtggt gggtcttctt cgcgatcgcg cagggaggct atctcttgac
    12841 cggtggccgt tacgtgcaag gcggtccgca acggctctcc agcgcgcttg cccgcgccat
    12901 caagctggcc ggcggagagg tgctgatgcg ccgcgccgtc agccgcatcg ccccgggttt
    12961 gcagggccat tcgagcacgg tcacgcacac cgcgcgcgat ggcagcgatg cccaggcggt
    13021 ggagtgcgta cggatcgtcg ccaatgccgc gcctgccgcg ctggcgccat tgatgcccgc
    13081 tacggccgcg gaaaaattga ccgcaaacga ccggcggcac atgccatcga tttcgctgtt
    13141 cgcgctcacg ctcggcctat cgagaccgcc gcgcgaattc gggatctcgg cctattcgac
    13201 gcaactgctg ccgccggaaa tggcgcggct gtccgactac gcgcaaggca ccgcgctgat
    13261 ggctgaggaa cccggggaac gaatgccgcc gatgtcgatc gtcgattacg ccgcgatcga
    13321 ttccggcgtc cctgctccgc cctatgtgct gttgatctgt gggcccgacc ggctgtcgaa
    13381 ctgggacagt tcggacatgg acgcctatcg cgagaagcgc gggcgatggc agcaggcgat
    13441 cgtcggctat ctctcttcgg tttatccggg ccttgccgac gcggtggtgg cgtcgtcgtt
    13501 caataccgcg ctgtcggtcc gccaggatct caatgcgccc gacggcgccg tctatgggtt
    13561 cgcgccgacg ccgccgcgct cgatctggcg cctcccggtc cgctcgccgc gcaccgcggt
    13621 gccgggcctc tatctggcgt ccgcctatgc ggggtttggc ggctacaccg gcgtggtgca
    13681 ttccgctggc gcttgcgccg acatgatctt gcgcgagagc ctgagccccc gtttcgatag
    13741 aatcgagacg ggtctctagc ttcttgattt gatgcgtttt cttgacgcga accggtctcc
    13801 accccggatc aagtctaaag gcatgtttcg cttgaaaacg ctttggccag tcattcaatt
    13861 ggtattgata cggaatcgca gcgatttggt tcccatgaat gaaacaagat cgccctgccg
    13921 cttccaggta gcggcctcgc tcaatgctgc gaccagttca tcgtcagcct gcgcccgcgc
    13981 cggcgggcag gcgcgatcct ccaggggtcc tgcgacaaaa atcacggtat tgcctgcaac
    14041 ggaaaattga cccttgccgc ccttgcacca cagatcgagc gtcgcctcgc cgcggtcgcc
    14101 gatgtccagc gtcggtatcc gcttcgatcc gggctgacgc gccgcatcca gggtcatctc
    14161 caacccaaac ggaaactcgt cttcggcgca ggccgccggg atggctttga aggtggccgt
    14221 ggccagcacg agcaccacgg tgacctgccc caaaaatctt gtcgctagcg acatacaact
    14281 cctcgaacta ttcggacact tacagctgcg cgtcgttcta gcgcatgatc cggcaaagtg
    14341 tgagcggttt gccgaaaaag atcgcgcgca cgtacaaaaa aattttgagc gcgatctcag
    14401 gaccaacgaa gacatttgtc ccataaaaag ccggattcgt tttgagggtc gcgcgcgatc
    14461 ggccgcgcga ccttgtccgg actggcagtc acaaaattca tcggctcaca ggcagatccc
    14521 cgcggcgcgg gccgcgggga tctgggtgtc aagcccggcg gcttacttca ggaccatctc
    14581 cgtaaaggga tagacatagg cctgcagcat gacgagcagg ccgacgagac acgccagcgc
    14641 gatcgaatgc ttgaacacga aacgcaggat cgtgctttcg tgtccgtacc agttggtggc
    14701 ggtggaggcg accacgatcg actgcgcatc gatcatcttg cccatcacgc cgccggagga
    14761 gttggccgcg cccatcagca ccgacgacag tcccagttgc tccgaggtga tcttctgcag
    14821 attgccgaac agaatgttcg acgccgtatc cgatccggtc agcgccacgc ccaaccagcc
    14881 gagcaaggtg ccgaagaagg gatacagcac gccggtcgcc gcgaatgcga gacccagcgt
    14941 tgcatcgacg cccgacagcc gcgtcagcgt gccgatcgcc agcatcgccg agatggtgat
    15001 cagcgagatc gcgcacagcc tgatggtgcg gccatacacc gcaatcatcc tggccggcga
    15061 gaagcccgcc agaaagccgg caatgatcgc ggcgatcagc atgccggtgc cggtgaacga
    15121 cagatagaag aagtcaaacg acgcgccttg cggcgtcggc ttcgccgcca ccggcggcat
    15181 cgtattgatc atgttgtgca gttcggggat cggatactta tagaagaaga tcgagttcgc
    15241 ccaggtcttg aacgccccgt tgccccagat cagcatcacg atgcatacga tgatccatgg
    15301 caagagcgcg ctgaacagct cgccttgcgt aagcgcggtc ttgtcgagcg gtatcgccgg
    15361 cttcatcgtc gcgatcgatt catccttgcc gcgcagcgcc ggcgacagcc acagcacctt
    15421 cggctgccag actttcagga acaggatcag acagcccatc gagatcagcg acgcgccgat
    15481 atcgacgatc cagggattga tgtaattgga aatgacgaat tgcgggaccg cgaaagatac
    15541 cccggtgacg aggatcgccg gccagacatc cttcatgccc ttccagccgg caaacgccca
    15601 caccacccag aacggcacga tcagcgaaaa gaccggcaac tggcggccga ccatcgcgcc
    15661 caggatatac ggatcgagcc cggtgacctg cgccaggccc gcaatcggcg taccgagcgc
    15721 gccataggcc accggcgcgg tattggcgat cagcgacagg ccggacgcgg cgagcggcga
    15781 gaagccgagg ccgatcagca ccgcgccggt aacggcgacc ggcgtgccga agcccgaggc
    15841 tccttcgaag aacgcgccga acgaaaacgc gatcagcaac agttgcaggc gccggtcctc
    15901 ggtgacgccg ccgaccgcgc gcttcaacag gtcgaattgg ccggttgcaa ccgtaagctg
    15961 gtagagaaag atgacgttca gcacgatcca gccgatcggg aaaaacccgg cgacgatgcc
    16021 aagcagggag gcgcggatcg acatgcccgc cggcatggtg aagatgaaga tcgcgatcag
    16081 gttggtcagg atcaccgcga tgatggctgc gatgtgcgcc ttcactttgc cgctggcgat
    16141 caacacaaga agcgtgacca cgggaatggc agcggcaatg gtcgacaacg ttgcgttgcc
    16201 aagcgggtta tagatttgat tccacattgc ggttcctccc cacgccgtta tgcggcgccc
    16261 gaccagcgtg ggaggtcggg tgtgcctggc gtggtcgcgg gagtgtctct ggccatgcgc
    16321 gaaattgcct gcgaattcgg cagcaccaaa atccgctcac aacctcgcga agtaagagag
    16381 ccccccgccc ctcgccgtta ggtcgcatgc tagggtctag ccgcaggtcg cgacaagcgc
    16441 acgcagacca tgaaatctac aactttagta tagacgatcc caatccttcc gttttttcag
    16501 ggctttgaag cccttatcct cggagatgac aatctgtgaa caacatccgc tcgacgctcg
    16561 ccaccgtatg gcggatcgcc gctccttatt tccgctccga ggacaaatgg gccggccgcg
    16621 gcctgcttgt cgcagtgatc gccattgagc tggcgctggt cgcagacgat gtgctggtga
    16681 accagtggcg caatcgattc tacaac
//
LOCUS       DATNOI010000599        10890 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_955175,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000599 DATNOI010000000
VERSION     DATNOI010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10890
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955175"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VN968_24340"
     CDS             complement(<1..1472)
                     /locus_tag="VN968_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXL32395.1"
                     /translation="MKLRWPDVTQIAIVVTSLVAFVTGGHFAAKAVIEGQQSRQLEEL
                     TDVALRRSEVAVDFGAAALDELARRGPLSCDPASLQAVRLQVYQRSGVKDIRLVNRDG
                     SVICSAYSETLEFDNGWVDRPEMLLSKENHLLLFRVDQFNGFALGVLRDIDKKNSLVA
                     ILGINSYLFDIMPAELRGHSEVLLELNNGSGVGRFALKRHAELAQLVSFNNVSSRYPL
                     HATIHVERGALQRWNSEAYWPIMMIALGLGVAFGVLLTKTTARLEGPIADIDRGLARG
                     EFKPFYQPTFDLRTGAILGCEVLARRIREDGSIIPPMSFIPLAESSGRIESMTWQILE
                     AALKELYHRLREDKQFKLSFNVVPRHLVSDGFIETLRRVVAGTKVSARQVMLEVTERN
                     ELPDLDRAAAVVKELRELGFRVAMDDVGVGHNGLSQMKGLGANTIKIDKFFVDSITEG
                     GSAATIVETLARLARDLHMTVIAEGIETFEQVQALIACGV"
     gene            1721..2032
                     /locus_tag="VN968_24345"
     CDS             1721..2032
                     /locus_tag="VN968_24345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32396.1"
                     /translation="MSECGDLEIVYQNGKLTIQPLQLEIEDDEGDLTDFRRLHDPEAG
                     VAMMERNKDHSTLLHFVASSELAAFLEIPTAPLGLGTFDCLAKMPLKIASSDGSTADP
                     P"
     gene            complement(2067..2396)
                     /locus_tag="VN968_24350"
     CDS             complement(2067..2396)
                     /locus_tag="VN968_24350"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HXL32397.1"
                     /translation="MKAVDGYGDDSIRVIYDVANAHFIGEAPTDGLRRVRDRLSLVHF
                     SDTTRQSYKHDPLGCGDVPLAGIASVMKEIETTELPMLEVISLKPDSDIADSCGRLQL
                     AGFGGDA"
     gene            complement(2393..2899)
                     /locus_tag="VN968_24355"
     CDS             complement(2393..2899)
                     /locus_tag="VN968_24355"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HXL32398.1"
                     /translation="MKNPAAGFGVNTYSYIFDGSAADTVARLADQGYGGVELMFFPGH
                     LWPAELDALQLRGLRQLCEARLRLVAVNMPNVDMNIAAAAEEMRSYTLDLLIKFVRCA
                     GELGAGGIIVGPGKANPLFPMPRDRMISHFYRALDVLAPLARQVGTRLFISSRTCRLP
                     SCRMRSRS"
     gene            complement(2896..4644)
                     /locus_tag="VN968_24360"
     CDS             complement(2896..4644)
                     /locus_tag="VN968_24360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL32399.1"
                     /translation="MMSASDAYDVVVIGAGAGGMTAAAVAAAEGLSVLVIEKTEFVGG
                     TTSWSGGMVWIPANSKMKQAGLPDSLSDAASYLASTVPEIENADLRDTFLKRGPEAVE
                     YLGANTEVQLQPVKTYPDYYPEKPGATVGGRVLEPVTFDGARLGANFGRLRPPLPEFT
                     LFGGMMVNRLDIPHLRKFGRSFRSTLRSMRLVSQYALQRLRAPRGTTLHLGNALAARL
                     YASLLARNVDIWFGASVEHLLVEGDAVRGVGIAGSSGSRPIVARKGVVLATGGFSHDQ
                     NFRGRYFPSAAGSVSAASPAGTGDGLRIATAAGASVGTAVASPAYWVPASRFQRADGS
                     AGVFPHTVTDRAKPGVIAVNSYGKRFVNEALSYHEFVLAMLRDGNDAAIRSFYLVCDR
                     HFLWTYGLGRIRPFTLRLKRYVRSGELIEAPGIEALADLIGVERSALATTVERYNTHA
                     QMGLDPEFGRGTTIYQRHLGDAGHRPNPCVAPIEQPPFYALRIYPADLGTAIGLRTDC
                     HARVLRNNGTVIAGLYACGNDMGSIMNGNYPGPGITLGPALTFGYIAGRHLAQADAEA
                     RAQENGQEERQERGLA"
     gene            complement(4679..5065)
                     /locus_tag="VN968_24365"
     CDS             complement(4679..5065)
                     /locus_tag="VN968_24365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL32400.1"
                     /translation="MASENNDAFIRNIAEVPWREFPNHFGGALSKPLVMPETAGSRHL
                     DYRISMYQPMAHVARHKHQVQEQIYHVLEGEGLMEIAGKNHVVRKHDFVFLPPGVEHA
                     ISNSGLVDLVFLVITSPVSDDGKNVA"
     gene            complement(5098..6504)
                     /locus_tag="VN968_24370"
     CDS             complement(5098..6504)
                     /locus_tag="VN968_24370"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXL32401.1"
                     /translation="MTRNHQTLVVVGHGAAGLAAALSAAEQADGSGLRIDITLLEKSR
                     EAEAGGNTRWSPSYMRLDAPDRLGRGFEDDMQQASRGLADRNYFRALAENAVPTIGWL
                     QGHGVEFGIPFYYLSAGPARIQPVGGGGAIVEKLLGAAKRAGVKVRYESYVTRLVMAA
                     GHRVRGIEFQCSDGVTTMLDADAVILASGGFQGNAAMMRAQFGLGAETLRLISPGTGF
                     DTGDGIRIARDQGARVSGDWNGMHIEPVDPRARNSAPVVLVYPYGIVVDQEGRRFFDE
                     GGGLVHETWEVFARDIHFGRPKRIVYAILDSRLFEIEGYERAIRSEVPPFQTETLEGL
                     ATQIGVSPGNLRETVETFNAAATLDAARFDATRCDGLAAAGTLDPPKSNWARAITRPP
                     YLAYPLVGAVAYTFGGLATNERAEVLSGQGPMPGLYAAGETTGHFYRTAPNAVSVLRA
                     LVFGRLAGREAVSFLQTR"
     gene            complement(6530..7231)
                     /locus_tag="VN968_24375"
     CDS             complement(6530..7231)
                     /locus_tag="VN968_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL32402.1"
                     /translation="MLAGKKVIVVGGSSGIGLATAELAKREGADVIVASRNVDRLDAV
                     AARLNAIAIPADVTSDESIVNLFRRCGPVDHVVVTAAQLRSGPFKTVSMDDVRATMEG
                     KFWGAWRVARSAEIRPGGSLTLVSGYLSIRPRPNSAIVSAANGAIESLARSLALELAP
                     VRVNAVSPGIIDTPIRAAMPEEARRDMLAKTAASLPVGRVGVGEDIARQILAFMTIGF
                     ATGSIVYIDGGALIT"
     gene            complement(7295..9097)
                     /locus_tag="VN968_24380"
     CDS             complement(7295..9097)
                     /locus_tag="VN968_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXL32403.1"
                     /translation="MSPRNALNGAQVIVDYLIQEKVPQVFGLCGHGNIQFIDALYERA
                     DELKTISVHHESVAGFMADVYYRVSGRPTATFTSCGPGSANLPISLGNAFLDSVPFLA
                     VTGNVPTSQFNRGAFQELYRHYQADFPSTVRSYCKKVFQPTRGEMVPLAIRQAWKTMT
                     TGRPGPVVLDVPFDVFLESAAEEAPNAHAWNGNISSRCGADPEGVVKAVDMLLGAERP
                     VMIVGQGVRYGGAADELLKLAERLQIPVAASASGLGAIDCNHPLALGLVARAGHYQAN
                     HATRQADVLLAMGMRFDDRTSSSWIPGYSFTIPPTHLIHVDIDPEEIGRNYPVALGLM
                     ADVRTFLRQIHAELDRRADLSKQAGARKKWLAQIDTYRKEWDEFVAPGFTDDTIPINP
                     QRAALEIDKALPENAILVSDIGVHHNWLLSFCKPKRPDSLIGSMGFGPMGFGVAGVMG
                     AKFAAPDRPCVSVCGDGAFFMHANVLGTAVEYNLPVVWVVWNNYAYASIRGLQRGYLG
                     GRELATDFHDPITGARYNPDFAAMARSCGVEGVRVDRAGDLGEAIRKGITANKPYLVD
                     VDIAADINPSGAGIWELPGLGRSKPGIGTQFEPT"
     gene            9324..10781
                     /locus_tag="VN968_24385"
     CDS             9324..10781
                     /locus_tag="VN968_24385"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXL32404.1"
                     /translation="MSPVSAARFTAEAGVTEIPMLIGGEWRTAAESYEVRDPYRNTVV
                     ARAQRSALTDLDDALDAAVKAKAKAAATPAFERAALLRRAAKLLVERADMIAEIMSRE
                     TGKAIKDAKAEIIRSQDTLNLSAEEAVRIEGEHIPLDASAMGAGKICFMLRFPVGVVA
                     GITPFNAPVNLACHKIAPSIAAGNTLVLKAAPQSPGVIHALAEIFVDAGTPAGVLNVL
                     YGDTVGPALVSDPRVDFITFTGSPQAGAQIKAASGLRRVALELGGNGVTIVHEDADIA
                     EAAPVCARNSMRLAGQSCISVQTVYVHRSVYEKFLDIVVTEVRKLKLGDPLDATTDVG
                     TLIDEGAAQRVESWIKEAADGGAKILTGGRRHGAQIEPTVIAGAKPAMKVVCDEVFGP
                     VISLLPYDDIDEVFRTVSESRFGLQTGIFTKSMELAIRAVRNLRTGGVIINGSSTWRT
                     DQLAYGGVKDSGIGREGPRYSIRDMTEERMVLFNY"
BASE COUNT         2213 a         3388 c         3285 g         2004 t
ORIGIN      
        1 tcgacgccac aagctatcag cgcctgaacc tgttcgaaag tttcgatccc ttcggcgata
       61 acggtcatat gcaaatctcg tgcgagccgc gcgagcgtct ccacgatggt tgcggctgat
      121 ccaccttccg tgatcgaatc gacaaagaat ttgtcgatct tgattgtgtt ggcaccaagt
      181 cccttcattt gcgatagccc gttgtgtccg actccgacgt cgtccatcgc taccctgaaa
      241 ccaagttcgc gcagctcctt caccaccgcc gcagcccggt cgaggtcggg aagttcgttg
      301 cgctcggtaa cttcaagcat gacctgacgc gccgaaacct tggtgccagc gacgacacgg
      361 cggagcgttt cgatgaagcc gtcgctgacc agatggcgcg gcaccacatt gaacgacagt
      421 ttgaattgct tgtcttctcg aagacgatgg tacagttcct taagggcggc ttccaagatc
      481 tgccatgtca ttgactcgat gcggccgctg gattcggcga gcgggataaa actcattggt
      541 ggaatgatgc tgccgtcctc acgtatccgt cgagccagca cttcgcatcc gagaatggcg
      601 ccggtgcgca gatcaaatgt tggttgatag aacggcttga actcgcctcg agccaaccct
      661 cgatcgatat cggctattgg gccctccagt ctggccgttg tctttgtcaa gagcacacca
      721 aaggccactc cgagccccaa ggcgatcatc atgattggcc aataggcctc gctattccat
      781 cgctgcaagg cgccacgttc aacgtggatc gtcgcatgga gagggtagcg gctggaaacg
      841 ttattgaagc tgaccaattg cgccaactcg gcgtgacgtt tcaaggcaaa ccggccaacc
      901 ccggagccat tgttcagctc aagcaggact tcgctatggc cgcgcaattc ggccggcatg
      961 atgtcgaaca aataggagtt tattccgaga attgcgacca gggaattttt cttgtcaata
     1021 tccctgagaa caccaagggc gaacccgttg aactgatcga cgcgaaaaag cagcagatga
     1081 ttttctttgg aaagcaacat ttccggacga tcgacccatc cgttgtcaaa ttccagcgtc
     1141 tccgaatagg ccgagcagat caccgagcca tcgcgattga cgaggcgtat atccttcacg
     1201 ccggaccgtt ggtaaacctg aagacgaacg gcctgcagcg acgccggatc gcaactcagt
     1261 ggcccacgcc tggcaagctc atcgagagct gcagctccaa agtccactgc aacctcggag
     1321 cgccgcaaag cgacgtccgt cagttcctca agttgccgcg actgctgccc ctcaataaca
     1381 gccttcgcgg caaaatgccc cccggtcaca aatgcgacca ggctcgtgac gacgatcgct
     1441 atctgggtaa cgtccggcca ccgtagtttc atcggaaacg cgcgctcgag atgataatcg
     1501 tctcgactct aggcgggagc atctaatatt tctacgcggg agcatccaat atcgagtttg
     1561 cagggcttcg ccccccgatt ggatggcgca atggataacg tgacgtcacg cgccatggtt
     1621 attgatcggt aaattttcag atgtttaaac cttcagggag aaggccatca acgacgcagg
     1681 cgatcacggg gctgcccgtc cgcaacgttg cagcgcgccg ttgtccgaat gcggcgatct
     1741 ggagatcgtg taccaaaacg gcaagttgac catacagccg cttcagcttg aaattgaaga
     1801 tgacgaagga gatttaaccg acttccgtcg gctgcatgat ccggaagctg gcgttgcgat
     1861 gatggagcgg aacaaggacc actcaactct cttgcatttc gtggctagct cggaattggc
     1921 cgcttttctc gaaataccaa ccgctccgct cggtttggga acctttgact gccttgcgaa
     1981 aatgccgctc aaaatcgcat caagcgatgg ttcgaccgct gatccaccgt aacccaccgg
     2041 cgaccgcggt ggatggtgca cgcagttcac gcatcgccgc caaaccccgc tagctggaga
     2101 cggccgcagc tatcggcgat gtcggaatcc ggcttgagcg aaatgacttc cagcatcggc
     2161 agctcggtgg tttcgatctc cttcatgacc gatgcaatgc ctgccagcgg gacatcgccg
     2221 caccccaacg gatcgtgctt gtaactctgc cgggtggtat cggaaaaatg aaccaggctg
     2281 agccggtcgc ggacacgccg caatccgtcg gtgggggctt cgccgatgaa gtgcgcattc
     2341 gccacgtcgt agatcacgcg aatgctgtcg tcgccataac catcgacagc tttcatgagc
     2401 gactccgcat ccggcaggaa ggcaaacggc atgttctcga tgagatgaaa agtcttgtcc
     2461 caacctgccg cgccagcggc gccagcacat cgagcgcgcg atagaaatgg gagatcatgc
     2521 gatcgcgcgg catcggaaac agcggattgg ccttgccggg accgacaatg atcccgcctg
     2581 ctccgagctc accggcacag cggacgaatt ttatcagcaa atcgagcgta tagctgcgca
     2641 tctcctcggc ggccgcggcg atattcatat cgacattggg catgttgacg gccaccagcc
     2701 gcagccgcgc ttcgcagagt tggcgcaagc cgcggagctg caatgcgtcg agctcggccg
     2761 gccagagatg gccggggaag aacatcagct cgaccccgcc ataaccctga tccgccagcc
     2821 gtgcgacggt atcggcggcg ctgccatcaa atatgtaaga gtaggtattg acgccaaagc
     2881 cagcggccgg atttttcatg ctaatcccct ctcctgtcgc tcttcttgtc cgttttcttg
     2941 ggcacgcgcc tcggcatcgg cctgtgcaag atggcggccg gcgatgtagc cgaatgtcag
     3001 ggccggcccg agcgtgatgc caggccccgg ataatttccg ttcatgatcg agcccatgtc
     3061 gttgccgcag gcataaaggc ccgcgataac ggtgccattg ttgcgcagaa cccgggcatg
     3121 gcaatcggtc cggagcccga tcgccgtgcc gaggtccgcc ggatagatcc gcagcgcgta
     3181 aaacggtggc tgctcgatcg gtgcgacgca tggattaggc ctgtggccgg cgtcgccgag
     3241 gtgacgctga tagatcgtgg tgccgcggcc gaattccggg tcgagtccca tttgggcgtg
     3301 cgtgttgtat ctttccacgg ttgtcgcgag cgccgatctt tcgacaccta tcaaatcagc
     3361 cagcgcctcg atgccaggcg cctcgatcaa ttcgccgctt ctcacgtatc gttttagacg
     3421 cagcgtgaag ggcctgatgc ggccgagccc gtaggtccag agaaaatgcc gatcgcagac
     3481 gaggtagaac gaacggatcg ccgcatcgtt accgtcgcgc agcatggcga ggacaaactc
     3541 gtggtaggac aaggcctcgt tgacaaagcg ttttccgtaa ctattgaccg caatgacgcc
     3601 gggcttcgcc cggtcggtca ccgtatgcgg gaaaacgccc gcgctgccat ccgcgcgctg
     3661 aaagcgcgac gccggcaccc aataagcggg actcgcaacg gctgtaccga cactcgcgcc
     3721 ggctgcagtt gcgatccgca agccgtcgcc ggttccggct ggagacgcgg cggaaacaga
     3781 tccggcggcg ctgggaaagt aacgcccgcg aaaattttga tcgtgagaga agccgccggt
     3841 cgccagcacc acgcccttgc gcgccacgat cggccggctg ccggatgagc ctgcgatccc
     3901 tacacctcgc accgcgtcgc cttcgaccaa cagatgttcg acgctggcgc caaaccagat
     3961 gtcgacgttt cgcgccagca gcgaggcgta caggcgcgcc gccagcgcat tgcccagatg
     4021 cagcgtcgtg cccctcggcg cgcgaagacg ctgcagcgca tattgcgaaa ccagacgcat
     4081 cgaacgcagg gtcgagcgaa acgagcggcc gaacttgcgc agatggggga tgtcgagccg
     4141 gttgaccatc atcccgccga acaaggtgaa ttccggcagc ggcggacgca gccgcccgaa
     4201 gtttgctccg agcctggcgc catcgaaggt gacgggctcc agcacgcggc cgccgacggt
     4261 ggcgcccggc ttctccggat aataatcagg ataggttttt acgggctgca gctgaacctc
     4321 ggtattcgcc ccgagatact cgaccgcctc cggaccccgc ttgagaaatg tgtcccgcag
     4381 gtcggcgttt tcgatttcag gaactgtgct ggcgagatag ctggcggcat cggacagact
     4441 gtcgggcaag ccggcctgtt tcatcttgct gttggcggga atccagacca tgccgccgga
     4501 ccacgatgtc gttccaccga caaattcggt cttttcgatc acaaggacgc tcagtccttc
     4561 ggcggcagcg accgcggcag ccgtcatccc accggcgcca gcgccaatga cgacgacatc
     4621 gtaggcgtcg gaagccgaca tcacatctcc gaccatgcgg tcaagaatgc aaaaaagatc
     4681 aggcgacgtt cttgccgtcg tccgacaccg gtgacgtgat caccagaaac accagatcga
     4741 ccaggcccga attcgaaatg gcgtgttcga cgccgggcgg cagaaagacg aaatcgtgct
     4801 tgcgcacgac atggttcttg ccggcgatct ccatcagccc ctcgccttca agcacgtgat
     4861 agatctgctc ctgaacctga tgcttgtggc gggcgacatg cgccatcggc tgatacatcg
     4921 aaatccggta atcgaggtga cgcgagcccg ccgtctccgg catcaccaga ggctttgaca
     4981 gcgcgccgcc aaaatgattg ggaaattcac gccagggcac ttcggcgatg ttgcggatga
     5041 aagcatcgtt attttctgat gccattgtcg ttgttcctcc caagatcgtt tcggatgtta
     5101 ccgggtctgc aggaaactga cagcttcccg tcccgccagt cgtccaaaca ccagcgcgcg
     5161 cagcaccgaa accgcattcg gtgcggtgcg atagaaatgg ccggtggttt ctcccgccgc
     5221 gtaaaggccg ggcatcggcc cttgcccgga taaaacttcc gctctttcgt tggtggcaag
     5281 accgccaaat gtataggcga ctgcgccgac cagggggtaa gcgagatacg gcggccgcgt
     5341 gatcgcccgc gcccagttcg atttcggcgg gtccaaagtg ccggccgccg cgaggccatc
     5401 gcagcgcgtc gcatcgaacc gtgcggcgtc aagggttgcg gccgcattga acgtctcgac
     5461 cgtctctctc agattgcccg gggaaacccc gatttgcgtt gccagacctt cgagcgtttc
     5521 agtttggaat ggcggcactt ccgatctaat cgcacgctca taaccttcga tttcgaacaa
     5581 ccggctgtcg aggatcgcat agacaatacg cttcggtctt ccgaaatgga tatcccgggc
     5641 gaagacctcc caggtctcat gcacgagacc accgccttcg tcgaagaacc ggcggccttc
     5701 ctgatcgacg acgatcccat aaggatacac cagcaccacc ggcgcggaat tcctggctcg
     5761 cggatcgacc ggttcgatat gcatgccgtt ccagtcgccc gaaacgcgcg cgccctgatc
     5821 cctggcgatg cggatgccgt caccggtgtc gaatcccgtg ccgggagaaa tgagtctcag
     5881 ggtctcggcg cccagcccaa attgcgcgcg catcatcgca gcgttgccct ggaatccacc
     5941 cgaagcgaga atgacggcat cggcgtcgag cattgttgtg acaccgtcgc tgcactgaaa
     6001 ctctatgccg cggacgcgat gccccgccgc catcacgagc cgcgtcacgt aggattcgta
     6061 gcgaactttc acccctgccc gttttgcggc gccgagaagc ttttcaacaa tcgcgccgcc
     6121 gccgccgacc ggctggatac gggccgggcc ggcgctgaga taatagaagg gaatgccgaa
     6181 ttcgacgccg tggccctgca gccatccaat cgttgggacc gcattctccg ccagcgcgcg
     6241 aaaatagttt cgatcggcca atccacgcga cgcttgctgc atatcatctt caaaaccgcg
     6301 tccgagccga tcgggtgcat cgagccgcat gtaggaaggg ctccagcggg tgttgccgcc
     6361 ggcttcggct tcacgcgatt tttcaagcaa ggtgatatcg atccgcaagc cgcttccgtc
     6421 agcctgttcg gccgccgaaa gtgcggcggc aagaccggcg gctccatgtc cgacgacaac
     6481 cagtgtttga tgatttcgcg tcattcgagc acatcacggt tggccgcgat caggtgatca
     6541 gggcgccccc atcgatatag acgatcgatc ccgtcgcaaa gccgatcgtc atgaaggcga
     6601 ggatctgccg cgcgatatct tcgcccacgc cgacgcggcc aaccggcaga ctggctgccg
     6661 tcttggcgag catgtcgcgg cgcgcctcct ccggcatggc ggcgcggatc ggggtgtcga
     6721 tgatccccgg cgaaacggcg ttgacacgca ccggcgccag ttcgagcgcg agactgcgcg
     6781 ccagcgactc gatcgcgccg ttggccgcgc tgacaatggc ggaattcggc cgcggccgaa
     6841 tgctcaagta gccagagacc agcgtcagcg atccacccgg gcggatttct gccgagcgcg
     6901 cgacgcgcca ggcgccccag aacttgcctt ccatcgtggc ccggacgtcg tccatggaaa
     6961 cggtcttgaa aggacccgag cggagctgcg cggcggtgac cacgacatga tcgaccggcc
     7021 cgcaacgccg gaagagattg acgatgcttt cgtcgctggt gacatcggcc gggatcgcga
     7081 tggcgttcaa cctggctgcg accgcatcca ggcgatcgac gttacgggat gcaacaataa
     7141 catcggcccc ttcccgcttg gcgagttcgg cggtcgcaag accgataccc gaagagcctc
     7201 caacgacgat gactttctta cctgcaagca tgatgctgtc tccctggtgt tatgattttg
     7261 gcggagagaa atgtcgtgcc gacgtgaacg acgatcaggt cggctcgaat tgggttccga
     7321 tgcccggctt gctgcgtcca aggcccggaa gctcccaaat gccggcgccg ctcggattga
     7381 tatcggcggc gatatcgacg tcgaccagat agggcttgtt ggcagtgatg cccttgcgga
     7441 tggcttcgcc gagatcgccg gcgcgatcga cgcgcacgcc ctcgacgccg caggaccgcg
     7501 ccatcgcggc aaaatccgga ttgtaacgcg cgccggtgat cggatcgtgg aagtcggtgg
     7561 cgagttcgcg gccgccgaga taaccgcgct gcagcccgcg gatcgaggca taggcgtaat
     7621 tgttccagac cacccagacc accggcaggt tatattcgac cgccgttccc agcacgttgg
     7681 catgcatgaa gaacgcgccg tcgccgcata ccgacacaca gggccggtcg ggggcggcga
     7741 acttcgcgcc catcacgccg gcgacgccaa aacccatcgg cccaaaaccc atcgagccga
     7801 tcagggagtc cggccgcttc ggcttgcaga aactcaacag ccaattgtgg tggacgccga
     7861 tgtcgctgac gagaatggcg ttctcgggca gcgccttgtc gatctccagc gccgctcgct
     7921 gcggattgat cggtatggtg tcgtcggtaa aaccgggggc gacgaactcg tcccattcct
     7981 tgcgataggt gtcgatctgc gccagccatt tcttgcgggc accggcctgc ttgctgaggt
     8041 cggcgcggcg gtcgagttcg gcatgaatct gccgcaggaa ggtgcgcacg tcggccatca
     8101 ggccaagcgc taccggatag ttgcggccga tttcctccgg atcgatgtcg acgtgaatga
     8161 ggtgggtcgg cggaatggtg aaggaatagc ccggaatcca cgaactggac gtgcgatcgt
     8221 caaagcgcat gcccatcgcc agcagcacgt cggcctgacg ggtggcgtga ttggcctgat
     8281 aatggccggc gcgcgcgacc agaccgagcg ccagcggatg attgcagtcg atcgcaccaa
     8341 ggccgctggc cgaggccgcc accggaattt gcaggcgttc ggcgagcttc aacaactcgt
     8401 cagcggcgcc gccgtagcgc acaccctggc cgacgatcat caccggccgc tcggcgccga
     8461 gcagcatatc gaccgccttg acgacgcctt ccggatcggc gccgcagcgg ctcgaaatat
     8521 tgccgttcca ggcatgcgcg ttgggggctt cctccgccgc cgattccaga aacacatcga
     8581 agggaacgtc aagcacgacc gggcccggac ggcccgtggt catggtcttc caggcctgcc
     8641 ggatcgcgag cggcaccatc tcgccgcggg tcggctggaa caccttcttg cagtaggacc
     8701 gaaccgtgga gggaaagtcc gcctgataat gccggtacaa ttcctgaaac gcgccgcgat
     8761 tgaactggct ggtcggcacg ttgccggtca ccgccaggaa cggcaccgag tcgagaaacg
     8821 cattgccgag cgaaatcggc aggttcgccg aacccggccc gcaggatgtg aaggtggcgg
     8881 tcggccgccc cgaaacacgg taatagacgt ccgccatgaa gccggcgacg ctctcgtgat
     8941 gcaccgagat ggtcttgagt tcgtcggcgc gctcatacag cgcatcgatg aactggatat
     9001 tgccgtgacc gcacaggccg aacacctgcg gcactttttc ctgaatcaga tagtcgacaa
     9061 taacctgagc cccattgagc gcattgcgcg gcgacatcgg atctctccct accagtttga
     9121 cgatttgagg agatcggcgc agcctactcg ctgccgaaac tgcccaggcg cgcggcaccc
     9181 tatttcccgt aatgctgtac gtcaatttat attgtggtag actgccggcc gagaacacgg
     9241 ctaagccaga cgccgaacag cgccgctttc aggaaaacca gacctgaata ccagacctgg
     9301 tttgccgaca cgggaggtcc aagatgagcc cagtttccgc cgcaaggttt accgccgaag
     9361 cgggcgtaac cgaaattccg atgctgatcg gcggcgaatg gcgcaccgcc gcggagagct
     9421 acgaggtgcg cgacccctat cgcaacacgg tcgtcgcaag ggcccagcgc tcggccctga
     9481 cagacctcga cgatgcgctc gatgcggcgg tcaaagccaa ggccaaagcc gccgcgacgc
     9541 cggcctttga acgcgccgcg ctgttgcggc gcgccgcgaa gttattggtc gaacgcgcgg
     9601 atatgattgc cgaaatcatg tcccgggaaa ccggcaaggc gatcaaggac gccaaggccg
     9661 aaatcatccg ctcgcaggat accctcaatc tgtcggccga agaggccgtg cgcatcgagg
     9721 gcgagcatat tccgctcgac gccagcgcga tgggcgccgg caaaatctgt ttcatgctgc
     9781 gctttccggt cggtgtcgtc gcggggatca cgccgttcaa tgcgccggta aaccttgcct
     9841 gccacaagat cgcgccttcg atcgcagccg gcaatacgct ggtgctgaag gcggcgccgc
     9901 aatcgccagg cgttattcac gcgctggcgg agatctttgt cgacgccggc acgccggccg
     9961 gtgtgctcaa cgtcctgtac ggcgacaccg tcggtccggc gctggtgagc gacccgcggg
    10021 tcgatttcat cacctttacc ggttcaccgc aggccggggc gcaaatcaag gcggcatcgg
    10081 gcttgcgccg ggtcgcgctc gaattgggcg gcaacggcgt caccatcgtc catgaagatg
    10141 ccgatatcgc cgaagcggca ccggtctgcg cccgcaactc gatgcggctc gccggccaaa
    10201 gctgcatctc ggtgcagact gtctatgtgc accgcagcgt ctatgagaaa ttcctcgata
    10261 tcgtcgtcac cgaggtgagg aagctgaagc tcggcgatcc cttggatgcc acaaccgatg
    10321 tcggcaccct gatcgacgaa ggcgcagccc agcgggtgga atcctggatc aaggaggccg
    10381 ccgatggcgg cgcaaagatc ctcaccggcg gcagacgcca tggcgcccag atcgagccga
    10441 ccgtgattgc cggcgccaag ccggcgatga aagtggtgtg cgacgaggtg ttcggaccgg
    10501 tcatcagcct gttgccctat gacgacatcg acgaggtttt tcgaaccgtc agcgaaagcc
    10561 ggttcggcct gcagaccggg attttcacca aatcgatgga gctcgccatt cgtgcggttc
    10621 gcaacctgcg caccggcggc gtcatcatca acggcagctc gacctggcgc accgaccagc
    10681 tcgcctatgg cggcgtgaag gattcaggca tcggccgcga gggtccgcgc tattcgatcc
    10741 gggacatgac agaggaacgc atggtgctgt tcaattatta ggtctgatga ggtctgtatg
    10801 gagcgcccct gcacgggggt tgtatgaacg gccgccgcgg ccaatgatgg ggcggcagca
    10861 caattcggca ccgtcgcgtg gaggctaaat
//
LOCUS       DATNOI010000600         6054 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1123106,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000600 DATNOI010000000
VERSION     DATNOI010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123106"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VN968_24390"
     CDS             complement(<1..890)
                     /locus_tag="VN968_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HXL32405.1"
                     /translation="MRLPKDDSAVLAARWTEGVLLKRDVFSTVERGHFRSPDGEVDAV
                     LRRLDQVPWWCFLPARQLFARERRALARARGLHVGPELLWAGRQALVRGFIDGVALHL
                     AKPFGDAAYFRSAKQALRKLHRAGICHNDLAKEQNWLRGSDGRAYLTDFQLAACFGTR
                     GRLFRIAAYEDLRHLLKHKRSYAPEALTPKERKILARKSFVASLWLATGKKVYRAITR
                     GLFNFTDREGGGRRLVNDAPLLSDLIRKNPDVRDTAIVAFADRRTGVGLYAFVEADRT
                     ALEAQLRSDLAAANGPRPPE"
     gene            complement(898..2331)
                     /gene="trmFO"
                     /locus_tag="VN968_24395"
     CDS             complement(898..2331)
                     /gene="trmFO"
                     /locus_tag="VN968_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HXL32406.1"
                     /translation="MTGIRATSEIVNIVGAGLAGSEAAWQVAKSGIPVVLHEMRPGRM
                     TEAHRTDGLAELVCSNSFRSDDATNNAVGLLHAEMRRLDSLIMRAADANQVPAGGALA
                     VDRDGFSAAVTKALNDHPLIEIRRAVIDGLPPEDWRNVIVATGPLTSAPVAEAIRQLT
                     DENALAFFDAIAPIVHRDSIDMSVAWFQSRYDKAGPGGTGADYINCPMTRGQYDAFVD
                     DLIAGEKTEFKEWETSTPYFDGCLPIEVMAERGRETLRHGPMKPVGLTNLHDPAVKPY
                     AIVQLRQDNKLGTLYNMVGFQTKLKYGAQQRVFRTIPSLENAEFARLGGMHRNTFLNS
                     PKLLDSQLRLHVQPRLRFAGQMTGCEGYVESASVGLIAGLYAAADARSTSLAPPPATT
                     ALGSLLGHVTGGHIETIEPGARSFQPMNINFGLFPPLASPPTRNAEGTRLRGNEKTVA
                     KKQAISARALSDLDRWIAHNLRPAMAA"
     gene            complement(2464..2616)
                     /locus_tag="VN968_24400"
     CDS             complement(2464..2616)
                     /locus_tag="VN968_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HXL32407.1"
                     /translation="MLLSLIRMIKAFRDYQRNVNELSQLSDRELADIGLDRSDIPRVA
                     AGTYNG"
     gene            complement(2992..3765)
                     /locus_tag="VN968_24405"
     CDS             complement(2992..3765)
                     /locus_tag="VN968_24405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HXL32408.1"
                     /translation="MLMMPIASALAASCGTGSFDAWLTDFKNEAAAKGISQSAIASGL
                     TGVTLDQAVLSRDRSQRVFNQSFEEFSGRMVPPRLARGASMMKQYGSVLARIEQSYGV
                     PGEVLVAIWGLETDFGANNGKFPTIRSLATLAYDCRRSDTFRAELMDALRIVERGDLA
                     PSEMRGAWAGELGQTQFMPSSYIKFAVDFDKNGRRDLLHSAPDVLASTANYLAGYGWQ
                     RGKDWEPGSANFSVLQQWNKSEVYARTVASFATQLARAP"
     gene            complement(4097..5002)
                     /locus_tag="VN968_24410"
     CDS             complement(4097..5002)
                     /locus_tag="VN968_24410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HXL32409.1"
                     /translation="MAAPYPGNVTPLAQDAPAAPIIRTIELSDLNEALRLGWEDFKAV
                     PSHAIILCVIYPVLGLMLARTVLGYSVLPLLFPLAAGFALLGPFAALGLYELSRRREC
                     GEPATAWDALEVLRSPSFGAMLGLGTLLFALFVTWVATAQAIYVAAFGYEAAAGIPDF
                     AKRVLTTPQGWWLIVVGCGVGFLFAVVALCVSVVSFPLMLDRHAGAADAMVTSLRAVA
                     RNPLPMAAWGLIVAVLLVLGSLPLFLGLAVVIPLLGHATWHLYRKVIAPDPNPRPIPP
                     RPPRERRSAADFPAALFPWRRRTDA"
     gene            5319..5705
                     /locus_tag="VN968_24415"
     CDS             5319..5705
                     /locus_tag="VN968_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HXL32410.1"
                     /translation="MSIFGKIMSAIFGSRAGAAPAGGGAATGSGSAAGSSAPAAPAQS
                     VDVAPILDKAVAGKGEKLAWRTSIVDLMKALDIDSSLTARKELAKELGYTGDTNDSAS
                     MNVWLHKQVMIKLAANGGKLPPEIKH"
     gene            complement(5772..6050)
                     /locus_tag="VN968_24420"
     CDS             complement(5772..6050)
                     /locus_tag="VN968_24420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL32411.1"
                     /translation="MAAASVRDNKAQNRFELDVEGAVAFANYRLAPGAIIITHTETPR
                     ALRGRGIASELVQGALQLIRADRLKVVAGCGFVVDYLRKHPEFGDLVR"
BASE COUNT         1201 a         1872 c         1939 g         1042 t
ORIGIN      
        1 tgttccggcg gtcttggacc gttcgccgcc gcgagatcgc tgcgcagctg cgcttcgagc
       61 gcggtccggt cggcctcgac gaaggcataa agcccgacgc cggtgcggcg atcggcgaag
      121 gcgacgatag cggtatcgcg aacatcggga tttttcctga tcaggtcgct cagcagcggc
      181 gcgtcattga ccagccgccg gccgccgcct tcgcgatcgg tgaaattgaa caggccgcgg
      241 gtgatggcgc ggtagacctt tttgccggtc gcaagccaga ggcttgcgac aaaggatttg
      301 cgcgcgagaa tcttgcgctc cttcggcgtc agcgcctccg gtgcgtagct gcgcttgtgc
      361 ttgagcagat gcctgagatc ctcgtaagcc gcgatgcgga acagccggcc gcgcgtccca
      421 aagcaggccg cgagctgaaa gtcggtgaga taagcgcggc catcgctgcc gcgcagccag
      481 ttctgctcct tggcgagatc gttatggcag atgccggcgc ggtgcagctt gcgcagcgcc
      541 tgcttggcgg accggaaata ggcggcatcg ccgaacggtt ttgccagatg cagcgccacg
      601 ccgtcgataa atccacgcac cagggcctgc cggccggccc atagcaactc gggcccgacg
      661 tgcaggccgc gcgcccgcgc cagcgcgcgg cgttcgcggg cgaacagttg gcgcgccggg
      721 aggaaacacc accatggcac ctgatcgagc cggcgcaaca ccgcatcgac ttcgccatcg
      781 ggactgcgaa aatggccgcg ctcgacggtg gaaaacacgt cgcgcttgag caacacacct
      841 tcggtccatc gcgccgccag gacagcggag tcgtcttttg gcagtctcat gaagggctca
      901 cgccgccatg gccgggcgca ggttgtgggc aatccagcga tcgagatccg acagcgctcg
      961 ggcgctgatc gcctgcttct tcgccacggt tttttcgttg ccgcgaagtc gcgtgccctc
     1021 cgcgtttctg gtgggcggag aggcgagcgg cggaaacaac ccgaaattga tgttcatcgg
     1081 ctggaacgaa cgcgcccccg gctcaatggt ctcgatgtgg cccccggtga cgtgcccgag
     1141 cagcgaaccg agcgccgtcg ttgcgggggg cggcgccagc gaggtcgagc gggcatcggc
     1201 cgcggcatag aggccggcaa tcagcccgac gcttgcggac tccacataac cctcgcagcc
     1261 ggtcatctgt ccggcgaaac gcagccgcgg ttgcacgtgc agtcgcaact ggctgtccaa
     1321 tagttttggc gaattcagaa aagtgttgcg atgcatgccc ccgagccggg cgaattcggc
     1381 gttctcgagg ctgggaatgg tgcgaaagac acgctgctgg gcgccgtatt tcagcttggt
     1441 ctggaagccg accatgttgt agagcgtgcc gagcttgttg tcctggcgca gttgcacgat
     1501 ggcgtagggt tttacggccg gatcgtgcag attggtaaga ccgaccggct tcatcgggcc
     1561 gtgacgcagc gtctcgcggc cgcgctccgc catcacctcg atcggcaggc agccatcgaa
     1621 atagggcgtg ctggtttccc actccttgaa ctcggtcttc tcgccggcga tcaaatcatc
     1681 gacgaaagca tcgtattgcc ccctcgtcat cgggcaattg atgtaatcgg cgccggtgcc
     1741 cccgggacct gccttgtcgt agcgcgattg aaaccacgcc accgacatat cgatggaatc
     1801 ccggtgtacg atcggggcaa tcgcatcgaa aaacgccagc gcgttctcat cggtaagctg
     1861 gcggatcgcc tccgccaccg gcgcagaggt caaaggcccg gtggcgacga tgacgttgcg
     1921 ccaatcctcg ggcggcagtc cgtcgatcac ggcgcgccgg atttcgatca gcggatgatc
     1981 attgagcgcc ttggtgacgg cggcggaaaa tccgtcgcgg tccaccgcca gcgcgccacc
     2041 ggccggcacc tgattggcgt cggcggcgcg catgatcagc gagtccagcc ggcgcatttc
     2101 ggcgtgcagc aagccgaccg cgttgttggt ggcgtcgtcg gagcggaacg aattggagca
     2161 gacgagttcg gcaaggccgt cggtgcgatg ggcttcggtc atccggcccg ggcgcatctc
     2221 gtgcaggaca acagggatgc ccgacttggc gacctgccag gcggcttccg agccggcaag
     2281 gcccgcgccc acgatattta cgatttcgga agtggctctg atgcctgtca tggtcacaga
     2341 ggtagcgcgt ttccgggcgc agtggaatgc ggcgccggcc cgatggcaga tacgacaacg
     2401 cccgcggcga ggcgggcgtt atccgttcgt caatcggtca aaaacgggtc agccgccaaa
     2461 agatcagccg ttataggtgc cggctgcaac gcgcggaatg tccgagcgat caaggccgat
     2521 gtcggccagt tcgcggtcgc taagctggga caattcgttg acattgcgct ggtagtcccg
     2581 gaacgccttg atcatgcgaa tgagcgagag caacatggta gtctccttgt agtatcaacg
     2641 attcagctct tggaggagcc tcaccgttga agtgaatata ggggagggtt ccttgctaca
     2701 gaacctcaat tgctgcggtg cagctaggtt tggaatgcat agcaaaagca agaaacgatt
     2761 aactaatggg cggggctacc tatggcgtcg gccccaggtt ccagctttgg cctgcttcac
     2821 aatagtgtgg tgtaatcaca ggatcgccat aaggatagcc agcagcaatg ccgattggcg
     2881 atcactggtc gcggaaccgg cgcgcttgtc gcggggcttc agaggaccag ttcctatgcg
     2941 cgattcgcat gtactctcag atgaaatgaa tttcaaatca tccaaggttg ttcagggcgc
     3001 gcgggcgagc tgggttgcga acgatgcgac ggtcctggca tagacctcgc tcttgttcca
     3061 ttgctggagg acggagaaat ttgcgctgcc gggctcccag tccttgccgc gctgccagcc
     3121 atagccggcg agatagttcg cggtcgatgc cagcacatcg ggtgcgctgt gcaacaggtc
     3181 gcggcgaccg tttttgtcga aatccacggc aaatttgatg taggacgacg gcatgaactg
     3241 ggtctggcca agctcgccgg cccacgcgcc gcgcatttcc gacggcgcca gatcgccgcg
     3301 ctcgacgatg cgcagcgcat ccatcaattc cgcgcggaac gtgtccgacc gccggcaatc
     3361 gtaagccagc gtcgcaagcg agcgtatggt cggaaatttc ccgttattgg cgccaaaatc
     3421 cgtctccagc ccccagatcg ccaccagcac ctcgcccggc acgccgtagc tctgctcgat
     3481 gcgggcgagc accgaaccat attgcttcat catgctggcg ccgcgcgcga ggcggggcgg
     3541 gaccatgcgg cctgaaaact cctcgaaact ctggttgaag acccgctgcg aacgatcccg
     3601 cgacagcacc gcctggtcca gcgtcacgcc ggtcaggccg gatgcgattg cggattgcga
     3661 aattcccttg gcggcagctt cattcttgaa atcggtgagc caggcgtcaa agctgccggt
     3721 gccgcaggat gccgccagcg ccgacgctat cggcatcatc agcatggcgc ctaccgtgac
     3781 ggttctgaaa acagaatgag gggtcatcgg gaccatcagc tccgaaatgg ttctcgaatg
     3841 aagaacgttg cccgaccgca gaaatctccg cggcaaccgc ggccaaaaca aggctctaga
     3901 agggcctata gttccgaaag cgcacgatat cgtcgtggcc gggcaaaagc gcgaaacgcg
     3961 ccatcgcgtt cgatgtcccg gcctcaagta acccagacgt ggatgcccgg cacaatggcc
     4021 gatgctcggg ccggcgacgc cggacccgag tgccgggcat gacgagtttc ggtatcgcaa
     4081 tcgtataatc ctgggcctat gcgtcggtcc tgcgccgcca tggaaacaat gccgccggaa
     4141 aatccgccgc ggaacgccgc tcgcgtggcg ggcggggagg gatcggccgc ggattgggat
     4201 ccggtgcgat gaccttgcga tagagatgcc aggtggcatg gccgagcagc gggatgacga
     4261 cggcgaggcc gaggaagagc ggcagcgatc ccagcaccag caataccgca acgatcaaac
     4321 cccatgccgc catcgggagc ggattccgcg ccaccgcacg gagcgaggtt accatcgcat
     4381 cggcagcgcc ggcgtgacgg tcgagcatca acgggaacga gaccacgctg acgcacagcg
     4441 ccaccacggc gaacaggaaa ccgacgccac aaccgaccac gatcagccac catccctgcg
     4501 gcgtcgtcag cacgcgcttg gcaaaatccg gaattccggc cgctgcctcg taaccgaacg
     4561 ccgccacata aatcgcttgc gcggtagcca cccacgttac gaacaatgcg aacagcagcg
     4621 tgccgagccc gagcatcgcg ccgaacgagg gcgagcgcag cacctcgagc gcatcccatg
     4681 cggtcgccgg ctcgccgcac tcacgacggc ggctgagttc atagagaccg agtgccgcaa
     4741 aggggccgag gagggcaaaa cccgccgcca gtggaaacag cagcggcagc accgaatagc
     4801 caagcaccgt gcgcgccagc atcagcccaa gcaccggata gatcacacag aggatgatgg
     4861 cgtggctggg caccgccttg aagtcttccc agccgagccg gagcgcttca ttgaggtcgg
     4921 aaagctcgat ggtgcggatg atcggcgcag ccggggcgtc ctgcgccagt ggtgtgacat
     4981 tgcccgggta tggtgctgcc atgctcgcag tctcccttga cgcatcggcc acagctcgca
     5041 ccagacgaag gcacgcgcgg gccgcttttc agaaacgatc gcgatcaagg ccagacgcga
     5101 agacagaaac cgaaactggc cttcgaactg agccgtgagc gtactcccaa tcgccggagt
     5161 cgtccaatca gtcgagtcgt cgctcacgct tcggtgaatg cttatcgcgg cgtaagcccg
     5221 gatcagtaat tcagccgtca ttttgccttg gcaatgtagt ttggtgctgc ccggatcaaa
     5281 aagtgcgttc aagccttcac acttattggg agccaacgat gagtattttc ggcaaaatca
     5341 tgagcgcgat cttcgggtcc agggccggcg cggcgccggc cggcggcggt gccgctaccg
     5401 gaagcggatc ggccgccgga tcgtcggcgc cggccgcacc ggcgcagtca gtcgatgttg
     5461 cgccaatcct ggacaaggcg gttgccggca agggcgaaaa gctggcatgg cgcacctcga
     5521 tcgtcgatct gatgaaggcg ctcgatatcg actccagcct taccgcgcgc aaagaactgg
     5581 ccaaggagct tggttacacc ggcgacacca acgattccgc gagcatgaac gtctggctgc
     5641 acaagcaggt gatgattaaa cttgccgcga acggcggcaa gctgccgccg gagatcaagc
     5701 actagtcggg ctttgtctgc tgtcgtcccc gcgaaagcgg gacgaccgag agtttggcga
     5761 cgatgttgaa gtcacctcac caaatcccca aactctggat gcttgcgcag gtaatccacg
     5821 acgaagccgc agccggcgac gaccttcaat cgatcggcgc ggatcaattg cagcgcgccc
     5881 tgcaccaatt ccgaggcgat gccgcgcccg cgcagcgctc gcggcgtctc ggtatgggtg
     5941 atgatgattg cgcccggcgc gagacgataa ttcgcaaacg ctaccgcgcc ctcgacgtcg
     6001 agttcgaagc ggttttgcgc tttgttgtcg cgaaccgatg ctgcagccat atcc
//
LOCUS       DATNOI010000601         3220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_432079,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000601 DATNOI010000000
VERSION     DATNOI010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432079"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="VN968_24425"
     CDS             complement(<1..1083)
                     /locus_tag="VN968_24425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL32412.1"
                     /translation="MLAKELAEIVSGRALWTMLLLVCPLVGYSFVQAVSLYSEASAAA
                     SQSPVLATSLSPLDGILVPTFGSFYVAVTLLFPFVALRVLGQEKETGALRLLVQLPYR
                     SFTLVSLKLVAVLAAWALVSIPALSALAIWAILGGHLAPSETLNLLFGHLLYGLLVGA
                     IAVFAATISESSATAAIIALAVTIGSWVMDFTLAGHSGLSARVAQLSLTQTLRTFEQG
                     LLSAGLVLGITAVVCCLAALASVWLPPGTPLRAKFIRSVPCVIATVAALAIATQIRKS
                     VDVTEDRRNSFPPADQRLLATLRLPLVVTVHLAPEDPRYVDLQRNVLAKLERAMPNVS
                     VVLAGSRRDFSDKSPDNAYGEIEYVYG"
     gene            complement(1118..1858)
                     /locus_tag="VN968_24430"
     CDS             complement(1118..1858)
                     /locus_tag="VN968_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32413.1"
                     /translation="MIVASSARDGNPAHVLRVAHLAKHYDHEAAIADVTFSVIPGEVL
                     GIVGPNGAGKTTLLETLAGLLAASSGTVFWRGRELPAPRRKEALFYLPDGVKPYQDRF
                     AAEVVSFFASVYRRSDREVAARIDSVGLAPALGKRVHALSKGYTRRLLLVLGLLTPQD
                     MLLMDEPFDGFDLRQTRSIIEVIRSEAAKGRTFMLAIHQLADAERVCDRFILFAGGQV
                     RGTGTLDELRVQTGFPAGSLEEIFLALT"
     gene            complement(1855..3159)
                     /locus_tag="VN968_24435"
     CDS             complement(1855..3159)
                     /locus_tag="VN968_24435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL32414.1"
                     /translation="MTIETSVADKVGRWDVRRPSNVNLLYVARAVRGFGDGFAVIILP
                     AYLTELGFSTLQVGIVATSALLGTAVLTLCVGYFASRFDLRNLLLISALTMIATGVAF
                     ANVELFPLLVIVAFAGTTNPSSGDIGIFVPLEHAMLAQDAADPSRTEVFARYSLIGGL
                     SAAAGALVATAPDLLVSFGVGKIGALQLMFYVYAVLGLVGAALYRLLPHVRSRDTRQQ
                     PASALGPSRGIVYQLAALFSLDAFAGGFAVQSLVALWLFERFDLSLTAASVFFFWSNT
                     LAAFSYPVAARLAKRFGLVNTMVFTHIPSSICLILAAFLPNLTAVLVLLLIRAALSQM
                     DVPTRTSYTMAVVTPAERPAAASVTAVPRSLASAISPTLAGALLSTAFSGLPLLICGS
                     LKIVYDITLLLSFRHLKPPEERDARDEAVRTSAQECADGVDR"
BASE COUNT          741 a          972 c         1000 g          507 t
ORIGIN      
        1 gccatagacg tactcgatct ccccataggc attgtccggt gatttgtccg agaaatcccg
       61 ccggctgccg gctagcacga cagaaacgtt cggcatcgcg cgctcgagct tggcgagcac
      121 gttacgctgc agatccacat agcgtgggtc ttccggagcg aggtgcaccg tcacgaccag
      181 cggcagccgc agcgtcgcga gcaaccgctg atcggcggga ggaaaagagt ttcgccgatc
      241 ttccgtaacg tccaccgatt tcctgatttg tgtcgcgatg gcaagcgcgg cgactgtcgc
      301 aatcacgcat ggcaccgagc ggatgaactt tgctcgcaac ggggtaccgg gcggcagcca
      361 cacgctcgcg agtgcagcaa gacagcagac cacggccgtg atgcccagca cgagtccggc
      421 agagagcagc ccctgctcga aggtgcgcag ggtctgcgtt aacgacagct gcgcaacccg
      481 tgccgacaaa ccggaatggc cggcgagcgt aaaatccatc acccacgatc caatggtgac
      541 agcaagcgcg ataatggcgg cggtcgctga actctcagag atcgtagccg caaacacggc
      601 aatcgccccc accaagaggc catagagcag atggccaaag agaaggttta aagtctcgga
      661 cggcgcaaga tggccgccca gaatggccca gatcgcgaga gcggaaagcg cgggaatgct
      721 aaccaaagcc cacgccgcaa ggaccgcaac aagcttgagg gaaacgagtg tgaagcttcg
      781 atacggcagt tggacaagca ggcgcagtgc acccgtctcc ttctcctggc caagcacgcg
      841 aagcgccacg aagggaaaca acagcgtcac ggcgacgtaa aacgagccga aggtggggac
      901 caggatccca tcgagtggcg acaggctcgt cgccagtacc ggcgattgcg atgccgcagc
      961 actggcttcg ctatagagcg acacggcctg aacgaagcta tatccgacca gcgggcagac
     1021 tagcagcaac atggtccaca gcgcgcggcc gctgacgatc tccgccagct cctttgcaag
     1081 caacggatac aacggagccg cggatagctc gtatgcccta ggtgagcgca agaaaaatct
     1141 cctccagaga tccagccgga aatccggttt gcacgcgcaa ttcgtcgagc gtgccagtcc
     1201 cccgcacctg ccctcctgcg aaaagaatga atcggtcgca aacccgctcg gcgtcggcca
     1261 attggtgaat ggcgagcatg aaggtgcggc ctttcgcggc ctcgctgcgg atcacctcaa
     1321 tgatgctacg ggtctgccgg agatcgaagc cgtcgaatgg ctcgtccatg agcaacatgt
     1381 cctgcggcgt caggagaccg agtactagca gcagccggcg ggtgtaaccc tttgacaggg
     1441 catgcacgcg cttgccaagg gcgggcgcca gcccgacgga gtctattctt gctgcgactt
     1501 ccctgtcaga tcgcctgtag acgcttgcaa agaaggatac gacttcggcc gcaaaccgat
     1561 cctgataagg ctttacgccg tcgggcagat agaacagcgc ttcttttctg cgcggcgccg
     1621 gcaattccct gccacgccag aaaacagttc ccgaactcgc agcaagcagg ccggcaagcg
     1681 tttccagcaa cgtcgttttt ccggcaccgt tcgggcctac gatgccgagc acctcccctg
     1741 gaataaccga gaaggtaacg tccgcaatag cagcctcgtg atcgtagtgc ttggcaagat
     1801 gggcgacgcg cagcacgtgc gcaggatttc cgtcgcgtgc ggaactcgcg actatcatcg
     1861 atcaactcca tcagcgcatt cctgcgcgct tgtccgaact gcttcatcgc gagcgtcacg
     1921 ttcctcgggc ggtttcaggt gtcggaacga caacagcaac gtgatgtcgt agacgatctt
     1981 cagcgatccg cagatgagca gcggcaggcc tgagaacgcc gttgagagca aggcaccggc
     2041 cagcgtcggg ctgatcgcgg acgcgaggct gcgcggcact gctgttacgc tggccgcggc
     2101 cggccgctct gcaggcgtga cgacggccat ggtgtaggaa gtgcgggtcg ggacatccat
     2161 ctgcgatagc gcggcacgga tcaacagcaa caccaacacg gcggtcaggt tcggcaggaa
     2221 cgcggccagg atcaggcaga tgctcgacgg aatgtgggtg aacaccatcg tgttgacgag
     2281 gccgaagcgc ttcgccagcc gtgcggcgac gggataggag aaagcggcga gcgtatttga
     2341 ccaaaagaag aacacgctcg cagcggtgag tgagaggtcg aagcgttcga acagccacag
     2401 agcgaccagg gattgaacgg cgaagccgcc ggcaaacgca tccaggctga acagcgcggc
     2461 caattggtag acgatgccgc gcgacggacc tagggcagag gccggttgtt gccgcgtatc
     2521 tcggctccga acgtgaggca aaagccggta caaggccgct ccgacgagac cgagcacggc
     2581 atagacgtag aacattaact ggagcgcgcc aatcttgccg acgccgaacg aaacaagcag
     2641 atctggcgcg gttgcgacca gcgcgccagc cgccgccgac aatccgccga tcaggctgta
     2701 acgtgcaaaa acctcggtgc ggctgggatc ggccgcatct tgcgccagca tggcgtgttc
     2761 aagcggcaca aagatgccga tatcgcccga ggacggatta gtggtgccgg cgaatgcgac
     2821 gataacgaga agcggaaaca attcgacatt tgcgaacgcc acgccggtcg cgatcatggt
     2881 caatgcgctg atcaagagta ggttgcgcaa gtcgaagcgc gaggcgaaat aaccgacaca
     2941 gagcgtcaag accgccgtgc cgagcagcgc ggaggtcgcg acaataccga cctgcaatgt
     3001 tgaaaagccg agttcggtca gataggccgg caggatgatg accgcgaacc cgtcgccgaa
     3061 tccgcgtacc gcgcgcgcaa catacaagag gttgacgttc gatggccgtc ttacgtccca
     3121 tcgtcccacc ttgtctgcca cgcttgtttc aatcgtcatt gtgcttgctc gatggctcta
     3181 gccgccgaac cggccccgta tgtcggacgc caacggcgat
//
LOCUS       DATNOI010000602         4513 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1490874,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000602 DATNOI010000000
VERSION     DATNOI010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490874"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VN968_24440"
     CDS             <1..881
                     /locus_tag="VN968_24440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32415.1"
                     /translation="YKKNDQAFVEQKNGAVVRRLVGYGRFDGVETTRVLERLYAASRL
                     HTNFFQPSFKLKDKRREGAKVIKRYHVPATPYARALAHPKLSKGVKRRLRELYRTLDP
                     VALLAEMRDAQTELGTRVDARAGKLAAARPPAPATNAAAFAKRLGIDVHLGEQRIIHR
                     RMHKPYKKRVKMPSMLDPHLADIERWLAAEPRLTALAILGRLAEHSPEQFGSPQHTIV
                     QRLLRSLRRKAAETIIPRTAEGAAPAGDPGTGAGAACDGHSATSPVPPILRAMTQPRT
                     ARRSELHPSSENNILR"
     gene            1004..1318
                     /locus_tag="VN968_24445"
     CDS             1004..1318
                     /locus_tag="VN968_24445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXL32416.1"
                     /translation="MDEVFKALADASRRSLLDRLHTKNGQTLNELCDGLAMTRQAVTK
                     HLAVLEAANLVTTFKHGREKLHYLNPVPIHEIGERWIRKFERGKLAALSELKRQLEKR
                     DE"
     gene            1311..1781
                     /locus_tag="VN968_24450"
     CDS             1311..1781
                     /locus_tag="VN968_24450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXL32417.1"
                     /translation="MSKPEFVYTTYIETTAEKLWHALTDGAFTERYWFGHRVASDWKV
                     GSPYKFAKQGAGTIEGKVLISEPPKRLAYTWDPCSAEAKRERTSRVTFDLEPRGNVIK
                     LTVTHDNLDEGGKTLRDISGGWPMVIASLKSLLETGHALPADLPSCAAKEKTDA"
     gene            1774..2241
                     /locus_tag="VN968_24455"
     CDS             1774..2241
                     /locus_tag="VN968_24455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXL32418.1"
                     /translation="MRKPEFVYVTYIETTPEKLWEALTSSEFSRRYWFNTELKTDWKV
                     GSPFALVMDGTTTDVGEVLEFDPPRRLSYTFHHVLSEAARKERPTRVVFNLEPHGKLV
                     KLTLTHEDFEAGSVILDGISKGWPAILSSLKSMLESGTALAIPPAALGIEGFA"
     gene            2238..2750
                     /locus_tag="VN968_24460"
     CDS             2238..2750
                     /locus_tag="VN968_24460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXL32419.1"
                     /translation="MNIDQFKPAIVYTIYIASTPEKVWQALTTAEFSRKYFSGNAVEV
                     DLKVGGAFVVRQPDGSLHISGEVIECVSPRRLTVTFNVNWPALIEKLGPTLVTYEIEP
                     AGDVVKLTLIQSHDRPISDDILSGGRQGWPAILSSLKSLLETGEALVIKMAPPQQMLE
                     ALKQMGIGTP"
     gene            complement(2889..4485)
                     /locus_tag="VN968_24465"
                     /pseudo
     CDS             complement(2889..4485)
                     /locus_tag="VN968_24465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135870.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
BASE COUNT          903 a         1438 c         1372 g          800 t
ORIGIN      
        1 cctacaagaa gaatgatcag gctttcgtcg agcagaagaa tggcgcggtc gtccggcgac
       61 ttgtgggata cggtcgcttc gatggcgtcg agacgacacg cgtgctggaa cgcttgtatg
      121 ccgcatcgcg gctgcacacc aacttcttcc agccgtcctt caagctaaag gacaagcgac
      181 gcgagggcgc gaaagtgatc aagcgctatc atgttccggc cacgccctat gcgcgcgcac
      241 tggcgcatcc gaaattgagc aaaggcgtca aacggcgtct tcgagagctt tatcgcacgc
      301 tcgatcccgt ggccctgctg gccgagatga gggacgccca gacggagctc ggcacgcgcg
      361 tcgacgcccg agcgggcaag ctcgcggcag cccgcccacc ggctccggca acgaacgcgg
      421 cagcgttcgc gaagaggctc ggcattgatg tgcatctggg ggagcagcgc atcatccatc
      481 ggcgcatgca caagccatac aaaaagcgcg tgaaaatgcc atcgatgctc gatccgcacc
      541 tcgccgacat cgagcgctgg ctcgcggccg aacctcgtct gaccgcgctg gccatcctcg
      601 gtcgtctcgc cgaacactcc cccgaacagt tcgggtcgcc gcagcacacc atcgtgcagc
      661 gcttgttgag atcactcaga cgcaaagcag ccgagacgat catcccgcgc acggctgagg
      721 gtgcggctcc ggccggtgat cccgggactg gggccggcgc tgcgtgtgat gggcactccg
      781 cgacgtcccc agtccctccg atcctgcgag caatgacgca acctcggacc gcccgacggt
      841 cggagttaca tccgtcaagc gaaaataaca tcctcagatg aggcaatacg cgagcctgag
      901 ttcggatcct cgagggctgc aaaaatccct gctcggatga aacatatcga tggcgaaaac
      961 tcacccttga catgcaacca tttggttgcc tattatccct gccatggatg aggtcttcaa
     1021 agcgctggcc gacgcgtcga ggcggtcgct gctggaccgg cttcacacca aaaacggaca
     1081 gaccctgaac gaactctgcg acggcctcgc catgacgcgg caggccgtca ccaaacacct
     1141 tgctgttctc gaggccgcca acctcgtcac caccttcaag catggccgcg agaagctgca
     1201 ttacctcaac ccggtgccga tccatgagat cggcgagcgg tggatcagga aattcgagcg
     1261 cggcaagctc gccgcgctca gcgaattgaa acggcaactg gagaagcgcg atgagtaaac
     1321 cggaattcgt ctacaccacc tatatcgaga ccaccgccga aaaactctgg cacgccctga
     1381 ccgacggcgc cttcaccgag cgttactggt tcggccatcg cgtcgcttcc gactggaagg
     1441 tgggttcgcc ctacaagttt gccaagcaag gcgccggcac gatcgagggc aaggtcctga
     1501 tctcggaacc gccgaagcgg ctggcctaca cctgggatcc ctgctcagcg gaggcgaaac
     1561 gcgaacgcac ctcgcgcgtt accttcgacc tggagccgcg cggcaacgtc atcaagctga
     1621 cggtgacgca cgacaacctc gacgagggcg gcaagacgct gcgcgatatc tccggcggct
     1681 ggccgatggt gatcgcgagc ctgaagagcc tgttggaaac agggcacgcg ttgccggccg
     1741 atctcccctc gtgtgccgcg aaggagaaga ccgatgcgta agcccgagtt tgtctacgtc
     1801 acctacatcg agaccacccc ggaaaaattg tgggaagcgc tcaccagcag cgaattttcc
     1861 aggcgttact ggttcaacac cgaactgaaa accgactgga aagtgggatc gccgttcgcg
     1921 ctggtgatgg acggcaccac caccgatgtc ggcgaagttc tcgagttcga tccgccgcga
     1981 cgtttgtcct acacgttcca ccacgtcctc agcgaagcag cccgcaagga gcgcccgacc
     2041 agggtcgtct tcaatctgga gccgcacggc aaactcgtga agttgacgct gacacacgaa
     2101 gatttcgaag ctggcagcgt gatcctcgac ggcatctcca agggatggcc ggcgatcctg
     2161 tccagcctca agagcatgct ggaatccggc accgcgctgg cgatccctcc tgccgcgctc
     2221 ggcatcgagg ggttcgcatg aacatcgacc aattcaagcc ggctatcgtc tacaccatct
     2281 atatcgcttc cacgccggaa aaggtctggc aggcgctgac gacggcggaa ttcagccgga
     2341 aatacttttc cggcaatgcg gtcgaggtgg acctgaaggt cggcggcgcg ttcgtcgtgc
     2401 gtcaaccgga tggttcgctg catatcagcg gcgaggtgat cgaatgcgtt tccccgcgca
     2461 ggctcacggt caccttcaac gtcaactggc cggcgctgat cgaaaagctc gggccaacgc
     2521 ttgtgaccta tgagatcgag cctgccggcg atgtggtgaa gctgaccttg atccagtcgc
     2581 atgaccggcc gatcagcgac gacatcctct ctggtggccg acagggctgg cccgcgatcc
     2641 tctccagcct gaagagcctg ctggagaccg gcgaggcgct tgtcatcaag atggcgccgc
     2701 cgcagcagat gctggaggca ctgaagcaga tggggattgg cacgccgtag cggttcgaca
     2761 tattcagtcg tcatacgcgg gcttgacccg cgtatccatc gctcttcgca agactctttt
     2821 caaagtgatg gattgccggg tcaagcccgg caatgacgac cgaagccttt tcgaggcttc
     2881 gcacaaaatt atcccaacgc ccgcaattcg cgccgcagca cctttcccgt ggcggtcatc
     2941 ggcaaggtgt cggtgaattg cacggtgcgc ggatattcgt gcgccgcgag ctgcaccttt
     3001 acaaaatcct ggatttcgcg cgccagcgcg tcgccgggcg caaagcccgg acggagcacg
     3061 atccatgcct tgatcgattc ggtgcggatc ggatcgggaa tgccgaccac cgccgacatc
     3121 gccaccgccg gatgtttcat cagcgtgtgc tcgatctccg acggcccgac gcgatagccg
     3181 gccgtggtga tcacgtcatc ctcgcggctg acgtaccaga aatagccgtc ctcatcctgg
     3241 atgccgagat cgccggtcag gagaaattcg ccgacgtatt ttttcgttgt cgcttccggg
     3301 ttgcgccaat attcgagcat ggtgcacgga ttgggctggc gcacgccgat gatgccccgg
     3361 tttccccgcg gcaattcctc gccctgctca ttgatgatgc gcacgtcgaa tccgggcgtg
     3421 gccttgccca tcgagcccgg ccggatcgga aacagtcttg aattgctgcc gatcgcgagg
     3481 ttgcattcgg tctggccgaa tacttcgtgg gcgtcgatgc cgaaggtcgc gcgcacccag
     3541 ccgagcaatt cgccgccgag cgattcgccg ccggtaaaga tgctgcgcaa tttgacgccg
     3601 gcgtgtttga cgtcggcgat ccgcatcagc ttcagcgcgg tcggcggcag aaacacattg
     3661 cggatgccgt ggtcggccat catctgcatc gccgcctgcg gctcgaattt tcgcgcgcgg
     3721 tggccgacga tcggcacgcc gtgataccag gcggcgaaca gcgcgttgat cagcccgccg
     3781 atccaggccc agtcggccgg cgtccacatc aggtcacccg gcttgggcag gaaatcgtgg
     3841 cacatctcga cattcggcag atggccgagc accacgcgat gcgcatgcag cgcgcctttc
     3901 gggtttcccg tggtgccgga ggtgtagatg atcagggccg gatcgtcggc cgaggtatcg
     3961 acggtcgcga actcggcggg cgccgccgca agctcccgcc agaacggttt tgtgccggac
     4021 ggcgcgacat cgccgatgac gtagatgttc gccagatcca gcagccggtc gcggattttc
     4081 gcaagcttct gccacccggt ctcgtcggtg acgatcgccc tggcgccgga attcgagcgc
     4141 atcctcgccg aacagagcga acagcggtat cgacaccaag cctgcgcgga acgccgccat
     4201 atgggcgatc ggcagttcca gcgactgcga caggaacacc gcgacgcggt cgccgcgcac
     4261 aagaccatcg gctttcagca cattggcaaa acggcgggag aagccgctga tctcgtcgaa
     4321 cgaatggcgc gtcgcgctgc cgtcctcatc gacatagatc agcgcgagac gcccctggcc
     4381 atcggcatgg ccgtcgcagc acgcggtcgc gatgttgaac cgcgccggga tgtcccagcg
     4441 gaaatcgcga tagagttttg cgtagtcggt ggtttcggtg agcatggctc cagattagac
     4501 catcgccctt gca
//
LOCUS       DATNOI010000603         8008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1669091,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000603 DATNOI010000000
VERSION     DATNOI010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669091"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VN968_24470"
     CDS             <1..367
                     /locus_tag="VN968_24470"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HXL32420.1"
                     /translation="SSVPQDLQDIINANGGKMKPTWGVPVSEIQRGIKNGVRKINIDT
                     DNRMAMTGQIRKILNEDPSEFDPRKYLKPAMEAMTKLCKQRLQEFNTAGQASKIKKVL
                     TTAEMAKRYVKGELDPKVA"
     gene            510..1553
                     /locus_tag="VN968_24475"
     CDS             510..1553
                     /locus_tag="VN968_24475"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HXL32421.1"
                     /translation="MNLADLNTIALTMVAPGKGLLAADESTGTIKKRFDGINVVSTEE
                     SRRDYREMLFRSSEAMTRYISGVILYDETIWQNAKDGTPLVKIIEQSGAIPGIKVDEG
                     TQALPDCPGELVTAGLDKLAERLKKYYDRGARFAKWRAVIDIGGGIGDRNIPSMTAIH
                     VNAHALARYAALCQAAQIVPIVEPEVLMDGDHDIDRCYEVTQRVLNKTFQELRVQRVA
                     LEGMILKPNMAVAGKKSAKQASVEEVAEKTIRLLKACVPAAVPGIAFLSGGQSDQDAT
                     AHLDAMNRIAGLPWRLTFSYGRALQAAPQKAWAGKADNVAAGQRALTHRARMNSLATK
                     GEWTADLEQQKAA"
     gene            1556..2236
                     /locus_tag="VN968_24480"
     CDS             1556..2236
                     /locus_tag="VN968_24480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXL32422.1"
                     /translation="MANRPVPPRPVPRLYLATPVVDDPALLMASLPGLLAAADVAAVL
                     LRLKPTDQRTMISRVKALAPVIQDGGAALLLDGHVELVARAGADGAHLTGIEAMEDAM
                     PTLKPDRIAGVGGLATRHDSMAAGEAGADYVLFGEPDAKGQRPSVEAIAERLQWWAEL
                     FEPPCVGFAASREEAHEFAAAGADFVLVGDFIWNDERGAKAALMEAEQTIRQAQAAMS
                     GSARAEQG"
     gene            2246..3355
                     /locus_tag="VN968_24485"
     CDS             2246..3355
                     /locus_tag="VN968_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL32423.1"
                     /translation="MKILRPILIVASCLTFAANAAAQISLTPPGVDTPPADKPAAKAK
                     AKPAAAAKKPPTPAATPNPAAPAATAKPGAKPAAPTATVAPAPAPDDPRVDVVFGAYQ
                     RGQYKTAFDLASKRAQDNHDPKAMTMLGELYANAMGIKRDYAKAADWYRRAADGGDRE
                     GMFALAMLRLSGRGGAVNREEAVKLLASSAKLGNPKAAYNLALLYLDGQTLPQDLTRA
                     AELLRLAADAGNPEAQYALATFYKEGTGVKKDLDMAVRLLQAASLADNVDAEVEYAIA
                     LYNGTGTPKDEAAAIALLRKAAKQNSPIAQNRLARVLVTGQGVPMDKVEGFKWHIVAK
                     TAGRGDPMLDEALAALSPEDRAKAQQAARIWLGNR"
     gene            3454..4248
                     /locus_tag="VN968_24490"
     CDS             3454..4248
                     /locus_tag="VN968_24490"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXL32424.1"
                     /translation="MLHSALINVMVKAARRAGRSLKRDLGEVENLQVSLKGPANFVTM
                     ADRRAEEMLHSDLTKARPGYGFIGEEGGTREGDDKSHTWIVDPLDGTTNFLHGIPQFA
                     ISIGLQREGTVIAGLIYNPANDDLYIAERGKGAFLNDQRLRVAGRRNLGDCVIACGLP
                     HIGRGDHNLSRLEMTEIQNKVAGLRRFGAASLDMAFVAAGRLDGYWERNLQPWDMAAG
                     QIIVREAGGTVSGMAGDDDPLKTGNLICGNEFVHAELVKILKQLEK"
     gene            complement(4312..5511)
                     /locus_tag="VN968_24495"
     CDS             complement(4312..5511)
                     /locus_tag="VN968_24495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXL32425.1"
                     /translation="MSTPGLALHNLRTVVIVIVLAVHAVIAYLGSSPAFSFNFDDPPY
                     RWRSIPIVDSERWFGFDIFCAFQDVYLISLLFFLSGLFVWPSLARSGSRIFLRDRLLR
                     IGVPFALTVGLLMPLAHYPVYRVTAVDPGLASFWQHWLALPFWPAGPPWFLWVLLVFD
                     FTAAVFYQLAPASGEALGRSFGQLGTRPKPFVIALLSASALAYVPLALVFNPWDWFHI
                     GPFSFQLCRPLHYLVFFLAGAGVGAYGIERGLLAVDGWLARRWAAAGIAAMVAFVLWL
                     GMVGLAMAGEGQPPLAVQILEALSFVMCCSAGVLFMLALFLRFANRRLAALDPLKDKV
                     YGIYLIHYVFSIWLQYALLGLAMFAVVKAMIVFTGTLLMSWASVAALRRIPARAWINR
                     SWRRFAT"
     gene            5562..6695
                     /locus_tag="VN968_24500"
     CDS             5562..6695
                     /locus_tag="VN968_24500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL32426.1"
                     /translation="MSLKFRLASRNLFHDRLRFIATIVGIVFSIVLVTVQMGLYVGFG
                     RMVTTMIDHTSADLWIMPLGTKSFEDPSLLDGRQRFRAMAVNGVAEAAPLVIGFAQWR
                     LPGGGTTPVFIVGSELRGLGLRPWDLVAGRIDDLSIPGAVAIDQSYFARLGSSGMGAS
                     AEIRDQKVEVAAVTKGIRSFTTTPYVFTGLDRARAYTGISSEKASYLLVRVSPDAAID
                     SVRRELKENLSNLEVLTTAEFRDRSRSFWLFGTGAGAALFAGALLGIIVGTVIVAQTL
                     YSSTKDHLDEFATLRAIGSSGAYIYTVIIWQALLSAVIGFCGAAAIGAIVVKATAESA
                     LPIVMTPALTVGLFVLTVAMCVASAIAAIMQVMRIDPALVFTR"
     gene            6692..7414
                     /locus_tag="VN968_24505"
     CDS             6692..7414
                     /locus_tag="VN968_24505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32427.1"
                     /translation="MTEAVLDAVDVVKRLGRGAGEVEALKGVSLSLNGGELVLLMGPS
                     GSGKTTLLSVMGCLMTPTRGVVRVRGRSIAGAGPEDLAKLRRDHIGFIFQSYHLFPTL
                     TAEENVRLALDVRGQRFRRAKIAARESLAMVGLSHKTKAFPIELSGGEQQRVAIARAI
                     VGNATAILADEPTGALDTQNGQAIMAVLAQIAKDQSRGVLVVTHDPRILSFANRVVHI
                     EDGRIVREEAGEHGKEQRVLRS"
     gene            7411..>8008
                     /locus_tag="VN968_24510"
     CDS             7411..>8008
                     /locus_tag="VN968_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HXL32428.1"
                     /translation="MITPKVKTAFFATLNLAVLLAALAVPAAMTHAQTPARAATIDPA
                     EEKRWLAVAPGRVEPSSGLIRVAPPVTGVIGEVLVGAGATVFAGEPLIRLADQEAQAR
                     LAAAEAQVALRRRVRNKESAASGAAARRRAEDAVADTEIAVSEARSFVDKAAVERRAG
                     RGSDADVDAARSGLTRAQVRLVQQKAELRRIEADASLPT"
BASE COUNT         1616 a         2491 c         2599 g         1302 t
ORIGIN      
        1 gtcgtcggtg ccgcaggacc tgcaggacat catcaacgcc aatggcggca agatgaagcc
       61 gacctggggc gtgccggttt ccgagatcca gcgcggcatc aagaacggcg tccgcaagat
      121 caacatcgac accgacaacc ggatggcgat gaccggccaa atccgcaaga tactcaacga
      181 ggatccgagc gaattcgatc cgcgcaaata tctcaagccg gcgatggagg cgatgacgaa
      241 actttgcaaa cagcgcctac aggaattcaa caccgcgggt caggccagca agatcaagaa
      301 ggtgctgacc accgccgaga tggcgaagcg ttacgtcaag ggcgaactcg atccgaaggt
      361 cgcgtgacag gcgcccactc cggagcgagc cggcctcgcc ggcgcccgcg cacgtttatt
      421 ggcgttttct tgttggcatt ttccaggggt ttgcctgaga accgtccact ttgacttgca
      481 aggaaacagt gctttcggag gaggcatcga tgaatctcgc cgatctcaac acaatcgctc
      541 tgaccatggt cgcgccgggc aagggcctgc tcgccgccga cgagtccacg ggcacgatca
      601 agaagcgctt cgacggcatc aacgtggtat cgaccgagga gtctcggcgc gactatcgcg
      661 agatgctgtt tcgttccagc gaagcgatga cccgctacat ctcgggcgtc attctctatg
      721 acgagacgat ctggcaaaat gccaaggatg gcacgccgct ggtgaagatc atcgaacaat
      781 caggggccat ccccggcatc aaggtcgatg agggcacgca agccctgccg gactgcccgg
      841 gtgaattggt caccgccggg ctggacaagc tcgccgagcg tctgaagaaa tattacgacc
      901 gcggcgcgcg gttcgcaaaa tggcgcgcgg tgatcgatat cggcggcggc attggcgacc
      961 ggaacattcc gagcatgacc gcgatccacg tcaatgccca cgcgctcgcg cgttacgccg
     1021 cattgtgcca ggccgcgcag atcgttccga tcgtcgagcc ggaagtcttg atggatggcg
     1081 atcatgatat cgaccgctgc tatgaagtga cccagcgtgt tctcaacaag acgtttcagg
     1141 aattgcgggt ccagcgggtc gcgctggaag gcatgatcct gaaacccaac atggccgtcg
     1201 ccggcaagaa aagcgcgaaa caggcttccg tcgaagaggt cgcggagaaa accattcggc
     1261 tgctgaaagc ctgcgtgcct gcggcggtgc ccggcattgc ctttctctcc ggcgggcagt
     1321 ccgaccagga cgccaccgcg catcttgacg ccatgaaccg gatcgccggc ctgccgtggc
     1381 gtctcacctt ctcctacggt cgcgcgctgc aggcggcgcc gcagaaggca tgggcgggca
     1441 aggccgataa cgtcgccgcg ggccagcgcg cactgaccca ccgcgcgcgg atgaactcgc
     1501 tcgccaccaa aggcgaatgg acggccgatc tggaacagca gaaggcggca tagatttggc
     1561 caatagacct gttcctccgc gccccgtgcc gcggctctat ctggcgacgc cggtggtgga
     1621 cgatccggcg ctgttgatgg cgagcctgcc gggcctgctc gccgcggccg atgtcgcggc
     1681 ggtgctgctg cggctcaaac cgaccgacca gcgcaccatg atctcgcgcg tcaaggccct
     1741 ggcgcctgtg atccaggacg gcggtgctgc gctcctgctt gatggccatg tcgaactggt
     1801 ggcccgcgcc ggcgccgatg gcgcgcatct gaccgggatc gaagccatgg aggatgccat
     1861 gccgacgttg aaacccgacc gcatcgccgg cgtcggtgga ctggcgacgc ggcacgattc
     1921 gatggccgcg ggcgaagccg gcgcggacta cgtgctgttc ggcgagcccg atgcgaaagg
     1981 gcagcggccg tcggtcgagg cgatcgccga gcgactgcaa tggtgggctg agctgttcga
     2041 gccgccatgc gtcggctttg cggcgtcgcg cgaagaggcc cacgaatttg ccgcggctgg
     2101 tgctgatttc gtactggtcg gcgatttcat ctggaatgac gagcgcggtg ccaaggcagc
     2161 cttgatggag gcggagcaga ccatccgaca ggcgcaggcc gcgatgtccg gttcggccag
     2221 agccgaacag ggataacgcc ggaaaatgaa gatcctgcgt ccgatattga tcgttgcaag
     2281 ctgtctgaca ttcgcggcga acgcggcggc acaaatctcg ctgacacctc ccggggtcga
     2341 cacgccgccg gctgacaaac cagccgccaa ggcgaaagcg aagccggcgg cggcagccaa
     2401 gaagccgcca actcccgccg cgacgccaaa tccggcggct cccgccgcaa cggcgaagcc
     2461 tggggcaaag ccggccgcgc cgaccgcgac ggtagctccg gcccccgcgc ccgacgatcc
     2521 gcgtgtcgac gtggtgttcg gcgcctatca gcgcgggcag tacaagaccg cgttcgatct
     2581 cgcctccaag cgcgcgcagg acaaccacga tcccaaggcg atgacgatgc tcggcgaact
     2641 ttacgccaac gcgatgggga tcaagcgcga ctatgccaag gctgccgact ggtacaggcg
     2701 cgccgccgat ggcggcgatc gcgagggcat gttcgcgctc gccatgcttc ggctcagcgg
     2761 gcgcggcggc gccgtcaatc gcgaggaagc cgtgaaattg ctggcgtcct cggccaagct
     2821 cggcaatccg aaggcggcgt acaatctcgc gctgctctat ctcgacggac agacgctgcc
     2881 gcaggacctc acacgcgccg cggaattgct tcgcctcgcc gccgacgccg gcaatccgga
     2941 agcgcaatat gcgctcgcga ccttctacaa ggagggcacc ggcgtgaaga aagatctcga
     3001 tatggcggta cggctgctgc aggcggcatc gctggccgac aatgtcgacg ccgaggtcga
     3061 atacgccatc gcgctctata acggaaccgg cacgccgaag gacgaggccg ccgcgatcgc
     3121 gctgttgcgc aaggccgcca aacaaaacag tccgatcgca cagaaccgcc tcgcacgtgt
     3181 gctggtgacc ggacagggcg tgccgatgga caaggtcgag ggcttcaaat ggcacattgt
     3241 cgcgaaaacc gccggcaggg gtgaccccat gctcgacgag gcgctggcgg ccttaagtcc
     3301 ggaggatcgc gccaaggccc agcaggccgc gcgcatttgg ctcggcaaca gatgatccgc
     3361 cttgacgcca ccgcagccgc agggcaccca cagggaagaa cccccgccaa tcccagtcct
     3421 gacctgacgc tcaggtcatc ttgagccgag atcatgctgc attcagccct tatcaacgtc
     3481 atggtcaaag ccgcgcgccg cgccggccgc agtctcaagc gcgacctcgg cgaggtcgag
     3541 aatctccagg tgtcgctgaa gggaccggcc aatttcgtca ccatggccga tcgccgcgcc
     3601 gaggagatgc tgcactccga cctcaccaag gcgcggccgg gctacggctt tatcggcgag
     3661 gaaggcggca cccgcgaagg cgacgacaag agccacacct ggatcgtcga tccgctcgac
     3721 ggcaccacca atttcctgca cggcattccg caatttgcga tctcgatcgg cctgcagcgc
     3781 gagggcaccg tgatcgccgg cctgatctat aatccggcca atgacgacct ctacatcgcc
     3841 gagcgcggca agggcgcctt cctcaacgat cagcgcttgc gcgtcgccgg ccggcgcaac
     3901 ctcggcgact gcgtgatcgc ctgcgggctg ccgcatatcg gacgcggcga ccacaatttg
     3961 tcgcgcctgg agatgaccga aatccagaac aaggtcgcgg gcctgcgccg gttcggcgcg
     4021 gcctcgctcg acatggcttt cgtggcggcg ggccgtctcg acggctattg ggaacgtaat
     4081 ctgcagccgt gggatatggc tgcggggcag atcatcgtgc gcgaagccgg cggcaccgtc
     4141 agcggcatgg ccggcgacga cgacccgctc aagaccggca acttgatctg cggcaacgaa
     4201 ttcgtccacg ccgaactggt gaagattctg aagcagctgg agaagtaggt tccgcctgct
     4261 tgtttcgcgg cgcgaaaatc gtttgcggta aatgggcagg cggctcgatg gctatgtcgc
     4321 gaatcgccgc cacgagcggt tgatccacgc gcgcgccggg atgcggcgaa gtgctgcgac
     4381 cgacgcccag ctcatcaaga gcgtgccggt aaacacgatc atggccttga caacggcaaa
     4441 catcgcgagc ccgagcagcg cgtattgcag ccagatcgaa aaaacatagt ggatcaggta
     4501 aatgccatag accttgtcct tcaaaggatc gagtgcggcc aaacgccgat tggcgaagcg
     4561 caggaacagc gccagcatga acaggactcc ggcagagcag cacataacga aactgagcgc
     4621 ttccaggatc tgcacggcga gcggcggttg gccctcaccc gccatcgcaa gaccgaccat
     4681 gccgagccag agcacgaagg cgaccatcgc ggcgatcccc gcggcagccc agcgacgcgc
     4741 cagccaaccg tccaccgcca gcaaaccgcg ctcaattccg taggcgccga cgcccgcgcc
     4801 cgcgaggaaa aagacaagat agtgcagcgg ccggcaaagc tgaaacgaga acggcccgat
     4861 gtggaaccaa tcccagggat tgaaaacaag agccagcgga acataagcga gggcggaggc
     4921 cgacagcagg gcgatcacaa atggcttcgg acgggtgccc agttggccga aagatcgccc
     4981 aagcgcttca cccgaagcgg gagcgagttg gtagaatacg gccgcggtga aatcgaacac
     5041 cagcagcacc cacaggaacc atggcgggcc tgcgggccag aacggcaagg ccagccaatg
     5101 ctgccagaac gaggccaggc ccgggtcgac ggcggtgacg cgatagaccg ggtaatgcgc
     5161 gagcggcatc agcaagccaa ccgtcaaggc aaagggcacg ccgatccgca gcaggcggtc
     5221 gcgcaggaat atgcggcttc cgctgcgcgc caagctcggc catacgaaca gcccggagag
     5281 aaagaacagg agcgagatca ggtagacgtc ctggaacgcg cagaagatgt cgaacccgaa
     5341 ccagcgctcg gaatcgacaa tagggatact tcgccaccga tacggcggat cgtcgaagtt
     5401 gaatgagaaa gcgggcgatg aacccagata ggctatgacc gcatgcaccg ccagcacgat
     5461 gacgatgaca acagttcgca gattgtgcag cgcaaggccc ggcgtggaca tgtggcatca
     5521 aagcagatga aatggggtta aattcggatg gtccacaact catgtcattg aagttcaggc
     5581 ttgcctcgcg aaacctgttt catgaccggc tacgattcat cgcgacaatc gtcggaattg
     5641 tcttttccat cgtgcttgtg acggtgcaaa tgggcctcta tgtcggtttc gggcgcatgg
     5701 tcaccacgat gatcgatcat acttcagccg atctctggat catgccgctc ggcaccaagt
     5761 ctttcgagga tccttcattg ctggatggcc gtcagcggtt tcgggccatg gcggtcaatg
     5821 gagttgccga agccgctcct ctggtcatcg gcttcgcgca atggcgattg ccgggcggcg
     5881 ggacgacgcc ggtcttcatt gtcggctccg aactacgggg attgggattg cggccgtggg
     5941 atttggtcgc cggtcggatc gacgatttgt cgattcccgg tgcggtggcg atcgaccagt
     6001 cttatttcgc gcggcttggt agcagcggga tgggcgccag cgctgaaata cgcgaccaaa
     6061 aagtcgaggt ggcggcggtg accaagggca tccgttcgtt caccacgacg ccctacgtat
     6121 tcacggggct tgatcgagcg cgggcctata ccggaatttc atccgaaaag gcctcttatt
     6181 tgctcgtgcg cgtttcccct gatgcggcca tcgacagcgt tcgtcgagaa ttgaaggaaa
     6241 atctctccaa tctcgaggtc ctgacaaccg ccgagtttcg cgaccgcagc cggagctttt
     6301 ggctgttcgg gaccggcgcg ggggctgccc tgttcgccgg agctttgctc ggcatcatcg
     6361 tgggcaccgt catagtcgcg cagaccttgt actcgagcac caaggatcat ctggacgaat
     6421 tcgcaacatt gcgcgccatc ggatcgtccg gcgcatatat ctacacggtc atcatctggc
     6481 aggcgctgct cagcgcggtc atcggattct gcggcgccgc ggcgatcggc gccatcgtcg
     6541 tcaaggcgac cgctgaatct gcattgccga tcgtcatgac gccggcgctg accgtgggat
     6601 tgtttgtgct gaccgtcgcg atgtgtgttg cctcggccat tgccgcgatc atgcaggtca
     6661 tgcgcatcga tcccgctttg gtgttcactc gatgaccgag gctgtgctcg acgctgtcga
     6721 cgtcgtaaaa cgccttggcc gcggagcggg tgaagtagag gcgctcaagg gcgtaagctt
     6781 gtcgctcaac ggcggcgagc tggtgctgct catgggacca tcggggagcg gcaagaccac
     6841 gctgctctcg gtgatgggat gcctgatgac gccgacgcgc ggagttgtac gggttcgcgg
     6901 ccggtcgatc gccggtgcgg gtcccgagga tctcgccaaa ctcaggcgcg atcacatcgg
     6961 cttcatcttt caatcctacc atctgtttcc caccctgacg gctgaggaga atgtccgcct
     7021 cgcactggac gtgcgcgggc agcgattcag acgcgccaag atcgcggcac gggaatccct
     7081 ggcgatggtc ggcctgtccc acaagacgaa ggcgtttccc atcgagctca gcggcggcga
     7141 acaacagcgc gtcgcgatcg cgcgggccat cgtggggaat gcaaccgcga ttcttgcgga
     7201 cgaaccgacc ggcgcactcg acactcaaaa cggtcaggcc atcatggctg tgctcgccca
     7261 gatcgccaaa gaccaatctc gcggggtgct ggtcgtaacg cacgatccgc gtatcctgtc
     7321 gttcgccaat cgcgtcgtcc acatcgaaga cgggcggatc gtcagggaag aggccggcga
     7381 gcacggcaag gaacagaggg tgctgaggtc atgatcacgc cgaaggtcaa gacggctttc
     7441 tttgccactc taaatttggc cgtactgctc gccgcgctcg ccgttccggc ggcgatgacc
     7501 catgcgcaaa cgccggcacg cgccgcgacc atcgatccgg ccgaggagaa gcgatggctg
     7561 gcggtcgcac cgggccgggt cgaaccgtcg tcggggttga tcagggttgc tccgccggtc
     7621 acgggcgtta tcggcgaagt gctggtgggg gccggcgcca ccgtgttcgc cggtgaaccg
     7681 ctgattcgcc tcgccgatca ggaagcccag gcgcgacttg ccgccgcaga ggcgcaggtc
     7741 gcattgcgaa ggcgcgtgcg caacaaggaa tcagccgcgt ccggggctgc ggcgcggcgc
     7801 agggccgagg atgcggtggc cgatactgaa atcgctgtat ccgaggcgcg gtctttcgtc
     7861 gacaaggcgg ccgtcgaaag gcgggccggc cgaggttcgg atgcagatgt ggacgcggcc
     7921 cgttcgggtt tgacgcgtgc gcaagttcgc ctggtccagc aaaaagccga gcttcgcagg
     7981 atcgaggccg acgcgtcgct gccaacgc
//
LOCUS       DATNOI010000604         3999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_253035,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000604 DATNOI010000000
VERSION     DATNOI010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253035"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="VN968_24515"
     CDS             complement(<1..1046)
                     /locus_tag="VN968_24515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL32429.1"
                     /translation="MNFDFSDEQKQLRDEARKFLAEKCPPKAVRVVLDGKEPYDRELW
                     KGLAEMGFLGVAIPEEFGGAGAGHLELCVIAEEMGRALAPVPFSSTIYLAAEAVLLAG
                     SDTQKRKWLPLIASGEAIGTLALFEGKGNPSPAGIKLAASGGMLNGVKKPVPDGAMAD
                     FAIVAARTGSTGRDSDISLFLVEMKAEGVEAKALTNIDPSRGQAELTFRNCKAEPLGA
                     ADEGWGILSEVLDRAAVLMAFEQVGGADRALEMGRDYALDRIAFGRPIGSFQAVKHML
                     ADMYVSATLARSNCYYGAWALSTNASELPEAAAAARISATQAFQLCAKNNIQVHGGMG
                     FTWEFDCHMYYRRA"
     gene            complement(1222..3096)
                     /locus_tag="VN968_24520"
     CDS             complement(1222..3096)
                     /locus_tag="VN968_24520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXL32430.1"
                     /translation="MSGNAAAVLTKPAFRRIEWLDRDIAIERRPDGVIVLKSRIPLKA
                     YEKHIPASLAKWAKENPNRIWLAQRAGADRQWRKLSYGEAKRTVDALTQAILDLKLEP
                     GRPVAILSGNSIEHALMTMASMQARFPAAPVSPAYSLMSQDHVKLKYLFDLIKPAVVM
                     VQDGPTFEKALKALDLDGVTVIHVARPCDGIKSVAFADLAATPVTTEVEESIANIAPD
                     TIGKLLFTSGSTGMPKAVINTQAMMCANAAMMMQVRPRDSGAPQATYLDWMPWNHTMG
                     GNALFNPVLTEGGTLYIDDGRPMPGQIDETLRNLREISPTYYANVPAGYAALAAAMEK
                     DDALCRSFFRNLGLMAYGGARLPDDLYDRMQALAVRATGERIVFYTGWGSTETGPTST
                     GTYWNTERVGLIGLPFPGVELKMVPVGAKYELRLRGVNVTPGYFGRPDLTKAAFDEEG
                     FYCIGDAGVFVDPSDPVQGIIFAGRVVEDFKLTTGTFVHVGSLRTDAIAAASPVVQDA
                     LVAGQDRPFVGLLAWPNLHACRQITGNPDASYEDVVKHPDVLACLKRGLQAHNASTEG
                     ASSLRIARAMLMTEPASIDGNELTDKGYINQRAGLGRRAALVERLYADRPGEEVIVLQ
                     "
     gene            complement(3303..>3999)
                     /locus_tag="VN968_24525"
     CDS             complement(3303..>3999)
                     /locus_tag="VN968_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL32431.1"
                     /translation="ARAMAAKGAKIAVLDQSKENAEKVAAELKGVAVHADVTDEEQVK
                     AGLAKAEAAHGVARVLMNCAGIGGSQRIVGRDGVYPLAKFVRIINVNLIGTFNVLRLF
                     AERLVTAEPIGEERGVIINTASVAAYEGQIGQIAYSASKGGVVGLTLPAARDLASQKI
                     RVNTIAPGLFLTPLLMGLNEEARKSLGAQVPHPARLGDASEYGNLAVHIVENPMLNGE
                     TIRLDGAIRMAPR"
BASE COUNT          689 a         1311 c         1239 g          760 t
ORIGIN      
        1 ttggcgcggc ggtagtacat gtggcaatcg aactcccagg taaaacccat gccgccatgc
       61 acctggatgt tgttcttggc gcaaagctgg aacgcctgcg tcgcgctgat gcgcgcggcg
      121 gcggcggctt ccggcagttc cgacgcgttg gtcgagagcg cccaggcgcc atagtagcaa
      181 ttcgaccgcg ccagcgtcgc cgaaacgtac atgtcggcaa gcatgtgctt caccgcctgg
      241 aacgagccga tcggccggcc gaaggcgata cgatccagcg cgtagtcgcg tcccatttcc
      301 agcgcgcggt cggcgccgcc cacctgctcg aacgccatca gcaccgcggc gcggtcgagc
      361 acctctgaca gaatgcccca gccttcatcg gcggcaccga gcggctcggc cttgcaattc
      421 ctgaaggtca attcggcctg gccgcgcgac ggatcgatat tggtcagcgc cttggcttcg
      481 acgccttcgg ctttcatctc gaccaaaaac agcgagatat cgctgtcgcg ccccgtcgag
      541 ccggtgcgcg cggcgacaat ggcgaagtca gccatcgcgc cgtcgggcac cggcttcttg
      601 acgccgttga gcatgccgcc ggaggcggcg agttttatcc ctgccggcga cggattgccc
      661 ttgccttcga acagcgccag cgtgccgatc gcctcccccg acgcaatcaa gggcagccat
      721 ttccgctttt gcgtatcgct gcccgcgagc aggacggctt ccgcggccag atagatcgtc
      781 gatgaaaacg gcaccggcgc cagcgcgcgg cccatttctt ccgcgatcac gcagagttcg
      841 agatggccgg cacctgcgcc gccgaattct tccgggatcg cgacgccgag aaagcccatc
      901 tcggcaagac ccttccacag ctcgcggtca tacggctcct tgccatcgag cacgacgcgc
      961 accgccttgg gcgggcactt ttcggcaaga aacttccgcg cttcgtcgcg aagttgtttt
     1021 tgttcatcgg agaaatcgaa attcatggcg ggttactcgg gttgggtgag gtgtatctct
     1081 tcagtacgtc gtccccgcga acgcgaggac ccataaccac aggcgtttgt gtttaatgcg
     1141 gacttggctc caacctaaca ttgatagtcc tcggcgtatg ggtccccgcg ttcgcgggga
     1201 cgacgttagt gggttgggga ctcattgcag cacaatcacc tcttcgccgg ggcgatcggc
     1261 atagagccgt tccaccaagg cggcgcggcg cccgaggccg gcgcgctgat tgatgtatcc
     1321 cttgtcggtg agttcattgc cgtcgatcga ggccggctct gtcatcagca tcgcgcgtgc
     1381 gatccgcagg ctgctggcgc cttcggtgga tgcgttatgc gcctgcaagc cgcgcttgag
     1441 gcatgccagc acatccgggt gtttgacgac atcctcatag ctcgcatcgg gattgcctgt
     1501 tatctggcgg caggcgtgca gattgggcca agccagcaat ccgacgaacg ggcggtcctg
     1561 ccccgcgacc agcgcgtcct gcaccaccgg cgaggcggct gcgatggcgt cggtgcgcaa
     1621 tgagccgacg tgcacgaagg tgccggtcgt cagcttgaaa tcctccacca cgcgccctgc
     1681 aaagatgatg ccctgcacgg ggtccgaagg gtcgacgaac acgccggcgt cgccgatgca
     1741 ataaaagccc tcctcgtcga acgccgcctt ggtgagatcg ggcctgccga aatagccggg
     1801 cgtgacgttg acgccacgca ggcgcagttc atatttcgcg ccgaccggga ccatcttcaa
     1861 ctcgacaccg ggaaacggca gcccgatcag cccgacgcgc tcggtgttcc aataggtccc
     1921 ggtcgaggtc ggcccggtct cggtcgatcc ccagccggta tagaacacga tgcgctcgcc
     1981 ggtggcgcgc acggccagcg cctgcatccg gtcatagaga tcatccggca gccgcgcacc
     2041 gccataggcc atcaagccca gattcctgaa gaaactgcgg cacagcgcgt cgtccttctc
     2101 catcgcggcg gcgagcgcgg catagcccgc gggcacgttg gcgtaatagg tcggcgatat
     2161 ctcgcgcaga ttacgcagcg tctcgtcgat ctggcctggc atcggccgac catcgtcgat
     2221 atagagcgtg ccgccttcgg tcagcaccgg attgaacagc gcattgccgc ccatggtgtg
     2281 gttccacggc atccagtcga gataagtggc ctgcggcgcg ccggaatcgc gcggccgaac
     2341 ctgcatcatc atcgcggcat tggcgcacat catcgcttgg gtgttgatga ccgccttggg
     2401 catcccggtc gagcccgagg taaacagcag ttttccaatt gtatccggcg cgatgttggc
     2461 gatcgattcc tcgacttctg ttgtcacggg ggttgccgcg aggtcggcga aggcaacgct
     2521 cttgatgccg tcgcaaggac gggcgacgtg aatgacggtg acgccgtcga gatcgagcgc
     2581 cttgagcgcc ttctcgaagg tcgggccatc ctgcaccatc acgaccgccg gcttgatcag
     2641 gtcgaacaga tatttcagct tgacgtgatc ctggctcatc agcgaatagg ccggcgacac
     2701 cggggcggcc ggaaaccgcg cctgcatcga ggccatcgtc atcagcgcat gctcgatcga
     2761 attgccgctg aggatcgcga ccgggcggcc gggctcgagc ttcagatcaa ggatggcctg
     2821 cgtcagcgcg tccaccgtgc gtttggcctc gccataggac agcttgcgcc actgccggtc
     2881 ggcgcccgcg cgctgcgcta gccagatccg gttcggattt tccttggccc acttcgcgag
     2941 ggaggccggg atgtgcttct cataagcctt cagcggaatc cgcgacttca ggacgatgac
     3001 gccgtcgggc cgccgctcga tcgcgatatc gcggtccagc cattcgatcc tgcgaaaggc
     3061 cggttttgtc aaaaccgcgg ctgcattccc actcatgtct cgtctcccaa agccctgtgg
     3121 tctcttctcg gaccttcgtt gttgcaccct ctccccttgt gggaaaggca tcaccggcag
     3181 tagcttcata ttcgcttggg tgaggggtct ctttctatcg atagaccgga acccctcacc
     3241 cgtcagcctt cggctgccac cctctcccac aaggggaccc acaaggggag agggaaggaa
     3301 aattaccgcg gcgccatgcg gatggcgccg tcgagccgga tggtctcacc attcagcatc
     3361 ggattttcga cgatatgcac cgcgaggttg ccatattcgg aggcgtcccc aagccgcgct
     3421 ggatgcggca cctgcgcgcc gaggcttttg cgcgcctctt cgttcagtcc catcaacagc
     3481 ggggtcagga acaagccggg cgcgatggtg ttgacgcgga tcttttggct ggcgagatcg
     3541 cgcgccgccg gcaacgtcag gcccaccacg ccgcccttgg aggccgagta agcgatctgg
     3601 ccgatctggc cttcataggc cgcgacgctc gcggtgttga tgatgacgcc gcgctcttcg
     3661 ccgatcggct ctgcggtcac gaggcgctcg gcgaacagcc gcagcacgtt gaaggtaccg
     3721 atcagattga cattgatgat gcggacaaac ttggccagcg gatagacacc gtccctgccg
     3781 acgatgcgct gcgatccgcc gatgccggcg cagttcatca gcacgcgcgc gacgccatgc
     3841 gcagcctcgg ctttcgccag tcccgccttg acctgctcct cgtcggtgac gtcggcatga
     3901 accgcgaccc ctttcaattc ggcggcgacc ttctcggcat tctccttgct ctggtcgagc
     3961 acggcgatct tcgcgccttt ggcggccatg gcgcgcgcg
//
LOCUS       DATNOI010000605         9816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1669488,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000605 DATNOI010000000
VERSION     DATNOI010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9816
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669488"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VN968_24530"
     CDS             <1..565
                     /locus_tag="VN968_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HXL32432.1"
                     /translation="GRKLEAGDELPVGAATTAVEEGRTVGEQLRGLPIAMPTELRVMP
                     GLYWHRLTEAAGKGFFADTWKVAPEADRIGYRFKGGKPLEFVPREPPFGAGSDPSNIT
                     DACYPYGSIQVPGGSEPIVLHRDAVSGGGYFMVGTVISADMDLIGQLQPNTPVKFVNV
                     EMDQALAARKGRADLLAKVRSSLTSSP"
     gene            1072..2016
                     /locus_tag="VN968_24535"
     CDS             1072..2016
                     /locus_tag="VN968_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32433.1"
                     /translation="MSLEWRARSSSLAWSNPVALWWGLLTMVSGANIAVWFLLYRELH
                     EQPTGSLGGTSGIQLMLLLCAAYVFGCAFRSFLPRADVQRICLFDTWLSSVVVGRSVA
                     TVAEISFAAQWAIILHQLGTMTGADTTLNAAWVILPLILIAECFSWYAVLTTHYLGNA
                     IENSIWAVAFFVVGVGLCRLLPEFEGPVRAVLVIAIIGIAGYLAFLMTIDIPMYLSRW
                     RAHVADGRKLLRLLEGLRDVSTRWIVTHDLAEWKDEIAWMSLYFSAAVWASLALCVFY
                     SLEDHLPRYRTEARVASWSSDAQAGASKQPPAMARHSF"
     gene            2154..3698
                     /gene="garD"
                     /locus_tag="VN968_24540"
     CDS             2154..3698
                     /gene="garD"
                     /locus_tag="VN968_24540"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HXL32434.1"
                     /translation="MQGNATTQAFEPRYIRLDANDNVAIVVNDLGLPAKTRFSCGLEL
                     RNFVPQGHKVALADIPEGAPIRRYNEVIGTAAQRILAGEWVDEARIQMPTPPELDKLE
                     LATAVPAPQAPLEGYTFEGFRNPDGSVGTKNVLAISTSVQCVAGTLQFAIKRIRAELL
                     PRYPNVDDVVGLTHNYGCGVAITAPLAIVPIRTLQNLALNPNFGGEVMVVGLGCEKLV
                     PERLLPDGADGHIVRMQDEAFDGFGAIVDAIMEMADARLAVLNRRRRETCPASDLVVG
                     LQCGGSDAFSGVTANPALGFAADLLVRAGASVMFSEVTEVRDAIHLLTRRAVNEDVGR
                     ALIREMDWYDSYLAKGGADRSANTTPGNKKGGLANIVEKSLGSIVKSGTSPIVDVLSP
                     GERVRRKGMTFAATPASDFICGTLQLASGMTLQVFTTGRGTPYGLAAAPVIKVSTRTE
                     LARRWKDLIDFDAGRIATGEATIEQTGWDLFRLILEVASGRTKVWSDHWGIHNDLTLF
                     TPGPVT"
     gene            3829..4731
                     /locus_tag="VN968_24545"
     CDS             3829..4731
                     /locus_tag="VN968_24545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXL32435.1"
                     /translation="MTNFPRRGLSRKQLAAAVLFCLASLPCLAAEEPDVPKDGPKGAA
                     VTVLKAAKSCFGNIVEVSGILIPKEETSVRPERPGLKVAEVLADAGDTVTAGQTLARL
                     TLPEGGNVQVQAPVAGLISTSSAVVGAPASGKGEALFSIIARSEFDLVGLVPVRDISK
                     LAVNQTARIKIIGSGEVDGKVRRVATTVEPNSQLGQVFVSVTTNRRLLVNSSGRALIK
                     TGQSCGVSVPLTAILYGPAGTVVQVVRRARVETRRVEVGLMSGGQVEIRDGLVEGDIV
                     VARAGALLREGDPVRPVTAASDVK"
     gene            complement(4857..5693)
                     /locus_tag="VN968_24550"
     CDS             complement(4857..5693)
                     /locus_tag="VN968_24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL32436.1"
                     /translation="MLDRAENQTGSPPADRDTAPRLVAFRGGGFTPRTGGTSGWIALA
                     LVIGFWQLAGSAGWVNPLFLPAPSAIAAAIAKLALSGALWQHLSVSIMRIGAGWILGT
                     IAGIIVGFAIGLSGLARGVGITFISALFPIPKIALLPLLILWLGIGEQPKIATIALGV
                     FFSTAISVYSGVDAVPRNLIRMAQSFNVPFHAIVRRVIWPGALPSILSGFRITASVAL
                     LLVVSAEMIGAEFGIGAFVLQAGNLMQTDQLLAGVVILSLFGLAVGRLINLLETRLLH
                     WR"
     gene            complement(5686..6459)
                     /locus_tag="VN968_24555"
     CDS             complement(5686..6459)
                     /locus_tag="VN968_24555"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32437.1"
                     /translation="MDLIADHISHRFGALAVLDDVSFTVRAGEVVAIVGPSGCGKSTL
                     LSILGGLLQPSGGRAEWRSAPPPGSLNPLTFVFQDFALLPWCTVEENVEFPLLHTGLD
                     LAARRAVIDDALRRTSLSDFRGTYPKKLSGGMRQRVGIARALAVRPAILLMDEPLSAL
                     DSQTRELLMEDFVRLLGGGAMGAVYVTHNLEEAVRLADRIVVLSRRPGKVREVVSIPL
                     TRGERGDINARGKLLALQGELWALIREEAIGAEREVQHA"
     gene            complement(6692..7738)
                     /locus_tag="VN968_24560"
     CDS             complement(6692..7738)
                     /locus_tag="VN968_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32438.1"
                     /translation="MTQRYRAGKARLGYARFAFASLLALTATVVASSSGRCDELLKAK
                     VGVLRLSSSAPVFIAQDKGYFREAGLEIELKFFDAAQPIAVATTSGDIDFGITAFTAG
                     LYNLAGKGTLKVIGGMSREKAGFPLIGYFASNSAYAAGLKTPRDLAGKRIAVTQTGSS
                     FHYSLGLLADKYGFKLSEVKVLPLQSLSNAVAALKGETVDAALLPASTARTLIDADGA
                     KLLGWVGDETPWQLGAVFASPKTLVNKPLVTKLLGALTRADREYHDVILAAVTGGKAP
                     INEVTKPLLEIIAKYTNLPVEQVVGNCAYIDPDGKLDVRNVSNQINWLQAQGFVDQGF
                     DADAIIAKDYVKPD"
     gene            complement(7978..9342)
                     /locus_tag="VN968_24565"
     CDS             complement(7978..9342)
                     /locus_tag="VN968_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL32439.1"
                     /translation="MPPKTIEEPARQVPLYGEYEVVILGGGPAGIAAAVAAARCGRRT
                     LLIERYGFLGGMGTAAGVTNFCGLHANVFGEMHRVVRGIASDLLDRIDRLGGLNAPHL
                     ILGKILAQAYDTAAYKIAADDLLLSHGVDILFHALGAGVVMHDARRINALMLETKAGR
                     RAVRAGIFIDCSGDGDLAAWAGARCEVGDNAGSMLYPSMMFRLNGIDPEKAGDAWRII
                     PALMEKAEAAGTHHFPRKAAIVRPQRSQIEWRVNFTQLAREDGTAINGLEPDDLTRGE
                     IDGRRQALNAYNFLRTVPGFEKSYIVDLPPQLGIRETRRVVGGYMLSGEDVLCCARFE
                     DSIGVNGWPMESHVAGDVIFKFPPIPESRGFNELPYRMLVPEGVDNLLVAGRCASMTH
                     DGQSAARVSGACFAMGEAAGTAAALALGGNTIPRDIAVDKLQLQLKHAGAFIGRDQVI
                     PEGL"
     gene            9680..>9816
                     /locus_tag="VN968_24570"
     CDS             9680..>9816
                     /locus_tag="VN968_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HXL32440.1"
                     /translation="MRICIFGSGAVGSHFAVRLALAGHDVSCVMRGPHLEAVRAKGLT
                     LR"
BASE COUNT         1800 a         3119 c         3099 g         1798 t
ORIGIN      
        1 gggtcgaaag ctcgaggccg gcgacgaact accggtcggt gcggctacga cagcggtcga
       61 ggagggccgt acggtcggag aacaattgcg cggcttgccg attgcgatgc cgacggagtt
      121 gcgcgtcatg cccggacttt attggcatcg cctcaccgag gccgccggca agggattctt
      181 cgctgatacc tggaaggtcg ctccggaagc cgaccggatc ggctaccgct tcaagggagg
      241 caagccgctc gagttcgtgc cgcgtgagcc gccattcggt gcgggctccg atccctccaa
      301 catcaccgat gcctgctatc cgtatggctc gatccaggtg cccggaggct ctgagccgat
      361 cgtgctgcat cgtgacgccg tttcaggcgg cggctatttc atggttggca cggtgatttc
      421 ggccgatatg gatctgatcg gacagcttca gcccaatacg ccggtaaagt ttgtcaatgt
      481 cgaaatggac caggcgcttg cggcccgcaa aggacgtgct gacctgctgg cgaaggtccg
      541 ctcgtccttg acgtcatcgc cctgaattcc cggacggagg caagaatgtt tgtccggcgg
      601 agctggcgca gcaggtgatc atgagcttca attcttcggc ctggtaatcc agaccctaag
      661 caagaagcga tgcgccgctt ttgcaaccgt atccgccggt agcggccatc ttcgctgacc
      721 agaccgcacg ctcaatcgcc ggcgcgatgc caacgagcgt aggccatctg gaaatcatgc
      781 ctctgcctgc gccagccctt ggcatcagcc ttggcaggga agcgtttcgc tcatacccga
      841 accctgcaca aagcccgctc ggggctcgcc ccgtgaagcc ggcttggctt cgagagtgct
      901 ggtcgcgagg ctttgtcccg gaccgaccca tgctgttggg accgaccaag gatgcgctcg
      961 ccatcacccc cgcaaggacc cgccacacac ttaatttttc tcgtgacggg ccgcgatcta
     1021 tgacagaatt aatgcaatcg ggtgtcagtt tggttacacg aggagcagtt catgagtttg
     1081 gaatggcggg cgcggtcaag ttccctagcc tggtcaaatc ccgtggcctt atggtggggt
     1141 ctgttgacca tggtcagcgg agcaaatatc gcggtttggt tcttgctgta ccgcgagctc
     1201 cacgaacagc cgaccggcag tctcggcggc acgtcaggca tccagctcat gctcctgctc
     1261 tgtgcggcgt atgtttttgg gtgcgcattc aggtcgttcc ttccgcgcgc ggatgttcag
     1321 cggatctgtc tgttcgacac ctggctgtcg agcgttgtcg tcggccggtc ggtggcaacc
     1381 gtggccgaga tctccttcgc ggcgcagtgg gcgattatcc tgcaccagct gggcacgatg
     1441 acgggagcgg acaccacctt gaacgccgcg tgggtgatct tgccgttgat tctcatcgcg
     1501 gaatgcttct catggtatgc ggtgttgacc actcactatc tgggcaacgc catcgaaaat
     1561 tcgatatggg cggttgcctt cttcgtcgtc ggggttggcc tttgccggtt gctgccggag
     1621 ttcgagggtc cggttcgcgc ggtcctcgtc atcgcgatca tcgggatcgc aggctatctg
     1681 gcgtttctca tgaccatcga catcccgatg tatttgagcc gatggcgggc ccatgttgcc
     1741 gacggcagaa agcttctgag acttctcgag ggtctccgtg acgtgagcac gcgttggatc
     1801 gtgacgcacg accttgccga gtggaaggac gagattgcct ggatgtcgct ttatttcagt
     1861 gcggcggtct gggccagcct cgcgctgtgc gttttctact ccctcgagga ccatctcccg
     1921 cgctatcgta ccgaagcgag ggtcgcaagc tggtcgtcgg acgcccaggc aggagccagc
     1981 aagcagcccc cggcaatggc gcgacacagc ttttaggcgc gcacgccagc ggattgcgcc
     2041 ggagcctgtc atcgggcggc gccttgcgcg aattctatgc aatcgtcaag acggcctata
     2101 tcctatccag gctgagcatg gcatggcgcg gcggcgatag agggaaggct ttcatgcagg
     2161 gaaacgcgac gacgcaggcc tttgagccgc gctacatcag gctcgacgcc aacgacaatg
     2221 tggcgatcgt ggtcaacgat ttgggactgc ctgcaaagac ccgcttttcc tgcggtctgg
     2281 agctgcgcaa cttcgtgccg cagggccaca aggtagcgtt ggccgatatc ccggaaggcg
     2341 ccccgatccg ccgttacaac gaggtgatcg gcacggccgc gcagcgaatc ctggctggag
     2401 aatgggtgga cgaggcacgc attcagatgc cgacgccgcc tgaacttgac aagctcgaac
     2461 tggcgactgc cgtccctgcc ccgcaagctc cgcttgaagg ttacaccttc gagggttttc
     2521 gcaaccccga cggttccgtc ggcacgaaaa acgtgctggc gatcagtacc agcgtgcaat
     2581 gcgtcgccgg cacgctgcag tttgcgatca agcgcatcag ggcggagctg ctgccaagat
     2641 atcccaacgt cgacgacgtc gtcggactga cgcacaatta tggctgcggc gtcgcgatca
     2701 ccgcgccgct ggcgatcgtg ccgatccgaa ccctgcaaaa cctcgcgctg aatccgaatt
     2761 tcggcggcga agtcatggtc gtcggcctcg gctgcgagaa actcgttccc gagcggcttt
     2821 tgcctgatgg cgccgacggc catatcgtgc ggatgcagga tgaagccttt gacggtttcg
     2881 gcgccattgt cgacgccatc atggaaatgg cggacgcacg ccttgccgtg ctcaaccggc
     2941 gccgccgcga aacctgtccc gcatccgacc tcgtcgtcgg cctgcaatgt ggcggctccg
     3001 atgcattttc cggtgtgacc gccaatcccg cgctgggctt cgccgccgac cttttggtgc
     3061 gcgccggcgc gtccgtgatg ttttccgaag tcacggaagt gcgcgacgcc attcatctgt
     3121 taacacgccg cgccgtcaac gaggatgtcg gccgtgccct gatccgcgaa atggactggt
     3181 atgacagcta tcttgccaag ggtggtgcgg accggagcgc caacaccacg cccggcaaca
     3241 aaaagggcgg gctggccaat atcgtggaga agtcgctggg ctcgatcgtc aagtccggca
     3301 ccagcccgat cgtcgacgtg ctgtcaccgg gcgagcgcgt gcgcaggaag ggcatgacgt
     3361 ttgcggcaac gcccgcgagc gacttcatct gcggcacact acagcttgcc tccggaatga
     3421 cgttgcaggt gttcacgacg gggcgcggta cgccctatgg gctcgccgca gcacccgtca
     3481 tcaaggtgtc gacgcgaacc gaactcgcgc gccgctggaa ggacctgatc gatttcgacg
     3541 ccggccgcat cgcgaccggc gaagccacga tcgagcaaac cggctgggac ctgttccggc
     3601 tgatcctgga ggttgcgagc ggtcggacca aggtgtggtc cgaccactgg gggattcata
     3661 acgatctcac tctgttcact ccggggccgg tgacctgagc cgaagaaggc gcggctattg
     3721 gcacttggcc gatgtcgaag gaccgccttg cgctccgatt tccattgccg cttggcgcct
     3781 cacccgccct tctgatatcg aacacccgaa accaaaactg gaacacccat gaccaatttc
     3841 ccccggcgcg gcctctcgcg aaaacagctc gccgccgcag tcctgttctg cctcgcctcg
     3901 ctcccatgcc tcgccgccga ggagcccgat gttcccaagg acgggccgaa gggcgcggcg
     3961 gtcaccgtgc tgaaagcagc aaaatcctgt tttggcaaca ttgtcgaggt ttccggcatt
     4021 ctgatcccga aagaagaaac gtcggtgcgg ccggagcggc cgggcctgaa ggtcgcggag
     4081 gttctggccg atgccgggga caccgtgacg gctggccaga cgctggcccg gctgacgttg
     4141 cccgaaggcg gaaatgtgca ggtgcaggcg ccggtggccg ggctgatctc cacctcctcg
     4201 gccgtcgtcg gtgcgcctgc ctcgggcaag ggcgaagcgc tgttttccat tatcgcgcgg
     4261 agcgaattcg atctggttgg cctggtgccg gtgcgggata tttcgaaact cgcggtcaac
     4321 cagaccgcgc ggatcaagat catcggctcc ggcgaggtcg acggcaaggt gcgccgggtg
     4381 gcaacgaccg tcgagcccaa cagccagctc ggccaggtgt tcgtctcggt gaccaccaac
     4441 cggcgcctcc tggtgaattc gtcgggccgg gcgctgatca agaccgggca gagctgcggc
     4501 gtgtcggtgc cgctcaccgc cattctctat ggccccgccg gcaccgtggt tcaggtggtg
     4561 cggcgggcgc gggtcgagac aagacgggtc gaggtcgggc tgatgtcagg cggccaggtc
     4621 gaaatccgcg acggtctggt cgaaggcgac atcgtggtgg cgcgcgccgg cgcgctattg
     4681 cgcgaaggcg atccggtgcg accggtaacc gccgcttcgg atgtgaaata gtttttcgtc
     4741 atggccgggc ttcgtcccgg ccatccacgt ctttctttgc ggctccgctg tcaagacgtg
     4801 gatgcccggg acaagcgcgg gcatgacgag ttttggtggc gcacaccgga aaactcctac
     4861 cgccagtgca gcaggcgggt ttccaacagg ttgatcagcc gccccaccgc gaggccgaac
     4921 agcgacagga tgacgacgcc ggccaacagc tgatcggtct gcatcaggtt gccggcctgc
     4981 aggacaaaag cgccgatgcc gaattcggcg ccgatcatct ccgcgctcac caccagcaag
     5041 agcgcgaccg acgcggtgat gcggaagccg gacaggatcg agggcagcgc gcccggccag
     5101 atcacccgcc gcacgatggc gtggaacggc acgttaaaac tctgcgccat ccggatcagg
     5161 ttgcgcggca ccgcgtcgac gccgctatag accgagatcg cggtggagaa gaacacgccg
     5221 agcgcgatgg tggcgatctt cggctgctcg ccgatgccga gccacaggat caacagcggc
     5281 agcagcgcga tcttcgggat cggaaacagc gccgagatga aggtgatgcc gacgccgcgc
     5341 gccagaccgg aaagcccgat cgcaaagccg actatgattc cagcgatggt gccgagaatc
     5401 cacccggctc caattcgcat gatcgaaacc gacagatgct gccagagcgc accgctgagc
     5461 gcgagtttgg cgatcgcggc cgcgatggcg gacggcgccg gcaggaacaa gggattgacc
     5521 cagccggcgc tgcccgccaa ttgccaaaat ccgatcacca gcgccagcgc gatccagccc
     5581 gaggtgccgc ccgttctcgg cgtaaaaccg ccaccgcgaa aggctacgag gcgtggcgcg
     5641 gtgtcgcgat cggctggcgg ggaacccgtt tgattttccg cgcggtcaag catgctgcac
     5701 ctcacgctcg gcaccgatcg cctcttcgcg gatcaacgcc cacagctcgc cttgcagcgc
     5761 cagcaattta ccgcgggcgt tgatatcgcc gcgttcgccg cgagtgaggg ggatgctgac
     5821 gacctcacgg acttttcccg ggcgccgcga cagcacgacg atgcgatcgg cgagccggac
     5881 cgcttcctcc agattgtgcg tcacatagac cgcgcccatc gcgccgcctc cgagcaggcg
     5941 gacaaaatcc tccatcagca attcgcgggt ctgcgaatcc agcgccgaca gcggctcgtc
     6001 catcaacagg atcgcgggcc gcaccgccag cgcccgggca atgccgacgc gctggcgcat
     6061 gccgccggac aatttctttg ggtaggtgcc gcggaaatcg gacaggctgg tgcggcgcag
     6121 cgcgtcgtcg atcaccgcgc gccgcgcggc gagatcaagg ccggtatgga gcagcgggaa
     6181 ttcgacattc tcttccacgg tacaccaggg caatagcgca aaatcctgga agacaaacgt
     6241 cagcggattg agactgccgg gcggcggcgc gctgcgccat tccgcccttc ccccgctcgg
     6301 ctgcaaaagc ccgccgagga tcgacaatag cgtgctcttg ccgcagccgg acgggccgac
     6361 aatcgccacc acctcgccgg cgcgaaccgt gaacgacacg tcgtcgagca ccgcgagcgc
     6421 cccgaagcgg tggctgatat ggtcggcgat aaggtccata aacgtttccg aagttcgcga
     6481 tccgcgcggc ttcgccgctt agttcgtcat gcccgggcat agtagtctgc cttgcgcaga
     6541 ctacgtaaac ttgtctgtgc tcccgggcat ccacgtctta acggcatagc agcaagaaag
     6601 acgtggatgg ccgggacatc tggcgcgaag acgcgcttcg cgcttttgcc cggccatgac
     6661 gaaaaagggg tgactggtcg cggctctccc atcaatccgg cttcacatag tctttggcga
     6721 tgatggcgtc ggcatcgaat ccctggtcga caaacccctg cgcctgcagc cagtttatct
     6781 ggttactgac gttcctgacg tcgagcttgc cgtcgggatc gatataggcg cagttgccga
     6841 ccacctgctc gaccggcaga ttggtgtatt tcgcgatgat ctccaacagc ggttttgtca
     6901 cctcgttgat cggcgccttg ccgccggtga cggcggccag gatcacgtcg tgatattcgc
     6961 ggtcggcgcg ggtaagcgcg ccgagcagtt ttgtcacaag cggtttgttg acaagcgttt
     7021 tcggcgaggc gaagaccgcc cctaactgcc acggcgtttc gtcgccgacc cagcccagca
     7081 gttttgcgcc atcggcatcg atcagcgttc gcgcggtcga ggccggcaac aatgcagcat
     7141 cgacggtttc gcccttgagg gcggccacgg cattcgacag cgactgcagc ggcagcactt
     7201 ttacctcgct cagcttgaag ccgtatttgt cggcgagcag gccgagcgaa tagtgaaagc
     7261 tggagcctgt ctgcgtcacc gcgatgcgtt tgccggcgag atcccttggg gtcttgaggc
     7321 cggcggcata ggcgctgttg ctggcgaaat agccgatcag gggaaagccc gccttctcgc
     7381 ggctcatgcc gccgatcacc ttcagcgtgc cttttcctgc gagattgtac agccccgcgg
     7441 tgaacgcggt gatgccgaag tcgatatcgc ccgacgtggt cgccaccgcg atcggctgcg
     7501 ccgcatcgaa gaatttcagt tcgatctcga gcccggcctc gcggaaatag cccttgtcct
     7561 gcgcaatgaa caccggcgcc gacgacgaca gccgcaagac gcctactttt gctttcaata
     7621 gctcatcgca acggccactg gatgacgcga ccacggtcgc tgtcaacgcg agcaggctcg
     7681 caaacgcgaa ccgtgcatat ccgagccgcg ccttacctgc gcgataacgt tgcgtcatca
     7741 agtctcctcc accttcatta gcccagatcc atcacggatt aagcattggc acgacgtatc
     7801 ggcaacggca gcgagctccc tcgcctcttg tgggagaggg ctggggtgag gggtcaaggc
     7861 ctatcgttgg gccctaaccc ctcacccgga tcgcatctcc gatgcgatcc gacctctccc
     7921 acaaggggag aggtgcactg aacatgccgc acgcgcaatt ccaattcacg attcagacta
     7981 caaaccttcg gggatcacct gatcccgccc gataaacgcc cccgcgtgtt tcaattgtaa
     8041 ctgcaatttg tccaccgcga tgtcacgcgg gatcgtattg ccgccgagcg ccagcgccgc
     8101 agcggtgcct gcggcttcgc ccatcgcaaa acaggcaccc gagacgcgcg ccgccgactg
     8161 gccgtcatgg gtcatcgagg cgcagcgccc ggccaccagc aggttgtcga cgccttcggg
     8221 aacaagcatc cgatacggca gctcgttgaa gccgcgcgac tccgggatcg gcggaaactt
     8281 gaagatcacg tcgccggcga catgggattc catcggccag ccgttgacgc cgatcgaatc
     8341 ctcaaaacgg gcgcagcaga gcacgtcctc gcccgagagc atatagccgc cgaccacgcg
     8401 ccgcgtttcg cggatgccga gttgcggcgg cagatcgacg atgtaggact tttcgaatcc
     8461 cggcaccgtg cgcaggaaat tgtaggcatt gagcgcctgg cggcggccgt cgatttcgcc
     8521 gcgcgtcaga tcgtcgggct cgaggccgtt gatggcggtg ccgtcctcgc gcgccaattg
     8581 cgtaaaattc acccgccact cgatttgcga acgctgcggc cgcacgatcg cggccttgcg
     8641 cgggaaatga tgggtgccgg cggcttcggc cttctccatc agagccggaa tgattcgcca
     8701 ggcatcgccg gctttttccg gatcaatgcc gttcaaacga aacatcatcg agggatacag
     8761 catgctgccg gcattgtcgc cgacctcgca gcgcgcgccg gcccaggcgg cgaggtcgcc
     8821 gtcgccggag cagtcgatga aaataccggc gcgcaccgcc cgacggccgg ccttggtctc
     8881 cagcatcagc gcgttgatgc ggcgcgcgtc atgcatcacc acgccggcgc cgagcgcgtg
     8941 gaagagaata tccacgccat ggctcaggag cagatcgtcg gcggcgatct tgtaggccgc
     9001 agtgtcatag gcctgcgcca gaattttccc caggatcaga tgcggcgcgt tgagcccgcc
     9061 aagccgatcg atgcgatcaa gcaggtcgga ggcgatgccc cgcaccaccc gatgcatctc
     9121 gccaaaaaca ttggcgtgca gtccgcaaaa gttcgtcacg cccgccgcgg tgcccatgcc
     9181 gccgaggaac ccatagcgct cgatcagcag ggtgcggcgg ccgcagcgcg ccgcagccac
     9241 cgcggcagcg atgccggcgg gcccgccgcc gaggataacg acctcatatt cgccatatag
     9301 cggcacctgc cgcgcgggct cttcgatggt tttgggcggc aagtcgcgga ttcctggttg
     9361 tcggcggcga ccatgccttg gctggggaat tttagccatt gcgaaggtga gtcagcgcgt
     9421 gctgaaactg ataactggcg tccgcgtcaa tggccataac ggcgggtttt ggttcggaac
     9481 atccgaacgc tcaacggcag aagacaatcc gaacaaactt cagtcagccc tcatgcgcat
     9541 tatgccgggc ggcgcgggcg cttgaaagct tacgcaccag cgtgtcggac atcccgccga
     9601 ccccgtgggt tgcactgccg gccgagcttg tggaaactag cctccgatca tcgtgcccct
     9661 aacaggaacc tgcatcccca tgcgtatctg tatctttggc tcaggcgccg tcggcagtca
     9721 ttttgcggtg cggctggcgc tggccggaca tgacgtctcc tgcgtgatgc gcgggccgca
     9781 tcttgaggcg gtgcgagcaa aaggcctgac gcttcg
//
LOCUS       DATNOI010000606         2801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2028160,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000606 DATNOI010000000
VERSION     DATNOI010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2028160"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="VN968_24575"
     CDS             <1..1291
                     /locus_tag="VN968_24575"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HXL32441.1"
                     /translation="CNLSRFCLMIGEQPVDHPLKAVQILQQKLGRKMPPKEISADQAI
                     CNQNVVEGDDIDIRIFPAQRMWPLDGGMYLGTGDAVVTKDPETGRINVGTYRMMIKGP
                     REVGVYTSPGKDATIDREKWWKMGKPMPIAAAYGIDPLLFLVAATSLPKTECEYDYYS
                     GINGAPIEVFTSDLTGLPLPARAEIVLEGFLYPDETFAEGPFGEFTGYYGRPSGATPF
                     MRVERVRYRDNPTLTCALMADTAANEAGLFWAALRSAGVWADLQKLGVPGIKGVWSIP
                     EAAGWGITVVSLKQMYAGHAAQVMALAAQCTAGAYFGKYVIVVDDDVDPTNVNQVLWA
                     MATRSRPAQSIDILRETWSTYLDPSLNPPEIRPWGSKCLINACMDYRFIKTFSKRTKL
                     SKDAYDNVVARWQELGLSGTPPVISVFEDEKSPEKIT"
     gene            complement(1336..1770)
                     /locus_tag="VN968_24580"
     CDS             complement(1336..1770)
                     /locus_tag="VN968_24580"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HXL32442.1"
                     /translation="MQFQRGQSGNPSGRPRGALNRATVLAQELLAARVESIAAKLIEL
                     AEGGDMRAIRVYMDRLMPVIKHQPIAVELPPIETAADSVEAAASIVAAVAAGELTAAE
                     AAELAKVIDTYVGALASRGFEERLTKLENESSGAPAGRAHAP"
     gene            2162..2476
                     /locus_tag="VN968_24585"
     CDS             2162..2476
                     /locus_tag="VN968_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXL32443.1"
                     /translation="MDQVFSALADASRRELLDRLRADNGQTLNELCARLDMTRQAVSK
                     HLGILEEANLVATVKQGREKLHYLNPVPIHDITERWIGKFERHRLQALSELKKGLEAR
                     DE"
     gene            2469..>2801
                     /locus_tag="VN968_24590"
     CDS             2469..>2801
                     /locus_tag="VN968_24590"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXL32444.1"
                     /translation="MSKPVFVYTIYIASTPEKVFKALTDTDVTAKFWFGNAVTSDWKA
                     GAPIEFRREGKLIVQGKVLEYDPPRRLSYTFQSMHEPFYGAEQPSRVVIDLEKQKDQV
                     KLTVTHDDF"
BASE COUNT          553 a          872 c          860 g          516 t
ORIGIN      
        1 atgcaacctc tcgcgcttct gcctgatgat cggcgagcag ccggtggatc atccgctcaa
       61 ggcggtgcaa atcctgcagc aaaagctggg tcggaaaatg ccgccgaagg agatttcggc
      121 cgatcaggcg atctgcaacc agaacgtcgt cgagggcgac gacatcgata tccgcatctt
      181 tccggcgcaa cgcatgtggc cgctcgacgg cgggatgtat ctcggcaccg gcgacgcggt
      241 ggtgaccaag gatccggaga ccggccgcat caatgtcggc acctatcgga tgatgatcaa
      301 aggcccgcgc gaggtcggcg tctacacgtc gccgggcaag gacgccacca tcgatcgtga
      361 gaagtggtgg aagatgggca agccgatgcc gatcgccgcc gcctatggca tcgatccctt
      421 gctgtttctg gtcgccgcga ccagcctgcc gaagacggag tgcgaatacg attattattc
      481 cggcatcaat ggcgcgccga tcgaggtctt caccagtgac ctcacgggcc tgccgctgcc
      541 ggcgcgcgcc gagatcgtgc tcgaaggctt cctctatccg gacgagacct tcgccgaggg
      601 accgttcggc gagttcaccg gctattacgg ccggccgagc ggggcgacgc cgttcatgcg
      661 ggtcgagcgc gttcgctacc gcgacaatcc gaccctgacc tgcgccttga tggcggatac
      721 cgcggccaat gaggccgggc tgttttgggc ggctttgcgt tccgccggcg tctgggccga
      781 cctgcagaag ctcggcgtgc ccggcatcaa gggcgtgtgg tcgatccccg aggccgccgg
      841 gtggggcatc accgtggtct cgctcaagca gatgtatgcc ggccatgcgg cgcaggtgat
      901 ggcgctcgcc gcgcaatgca cggccggcgc ctatttcggc aaatacgtca tcgtcgtcga
      961 cgacgacgtc gatcccacca atgtcaacca ggtgctctgg gccatggcga cgcgttcgcg
     1021 cccggcgcag tcgatcgaca tcctgcgcga aacctggagc acctatctcg atcccagcct
     1081 gaacccgccg gagatcaggc cttggggatc gaagtgtctg atcaacgcct gcatggacta
     1141 tcgctttatc aagacgttct ccaagcgcac caaattgtcc aaggacgctt acgacaacgt
     1201 ggtggcgcgg tggcaggagc tgggattgag cggcacgccg ccggtcattt cggtattcga
     1261 ggacgagaag tcgccggaga aaattactta gcaggcacga gtgatgcgaa ggcgaggacg
     1321 cgaatttgca gcgcttcacg gcgcatgcgc ccggccggca ggcgcgccgc tactctcgtt
     1381 ctcgagcttg gtcaggcgtt cctcgaaccc cctgctggcg agcgcgccca cgtaagtatc
     1441 gataaccttt gccaattcgg cggcctcggc tgccgtcagc tcgccggccg cgaccgcggc
     1501 cacaatgctt gccgccgcct cgacactgtc ggccgccgtc tcgatcggcg gcagctccac
     1561 ggcgatcggc tggtgcttga tcaccggcat aagccggtcc atgtacacgc gaatggccct
     1621 catgtcgccg ccctccgcga gttcgatgag ctttgcagcg atgctctcga cgcgtgcggc
     1681 caacagctct tgcgccagca cggttgcccg attgagcgcc ccgcgcggtc gtccggacgg
     1741 attcccgctt tggcctctct gaaactgcat ggctgtgatc tcctgtattt ccctgttttt
     1801 ttgcaaacac gcgaaacccg ccgcgcgcga ccgcgcaacg aaaaacccgg cgcggcatgc
     1861 cgcccgggct tctggcgtag tttcggtgaa tacgcttttc tcgaagattc tcgttacgcg
     1921 agtcaagcga aaaatgggct tgccgttaag tcgctgaatt agcgaagaat gtcggcgctt
     1981 ccccgagagg agacaaaaac ggcggccgtc tcggacgtcg gcgatgtgcc gcgaacagac
     2041 atatgcaacg caatcgcagc cttcccgcct gagctacacg cacagccaat ctggatcgcg
     2101 attgtctgaa ttggatcaac agcggtcttg acaggcaacc aaaaggttgc atattatgac
     2161 tatggatcaa gtgttcagcg ccctggccga tgccagccgc agggaattgc tcgaccggct
     2221 gcgcgcggat aacggccaga cgctgaacga actctgcgcg cgcctcgaca tgacgcggca
     2281 ggcggtcagc aagcacttgg ggatcctgga ggaggccaat ctcgtcgcca ccgtgaagca
     2341 gggcagagag aaactgcact acctcaaccc ggtgccgatc cacgacatca ccgagcgctg
     2401 gatcgggaaa ttcgaacgcc accggttgca ggcactgagt gaactcaaga aaggactgga
     2461 ggctcgtgat gagtaagccc gttttcgtct acacgatcta cattgcgtcg acgcccgaga
     2521 aagtcttcaa ggcgctgacc gataccgatg tgaccgcaaa attctggttc ggtaatgctg
     2581 tcacctctga ttggaaggcc ggcgccccga tcgaatttcg ccgcgagggc aagctgatag
     2641 tgcaaggcaa agtgctggaa tacgatccgc cgcggcggct atcctacacg ttccagtcga
     2701 tgcacgaacc tttctacggg gccgagcagc cctcgcgcgt cgtgatcgat ctcgaaaagc
     2761 agaaggatca ggtcaagctc accgtcacgc acgacgattt c
//
LOCUS       DATNOI010000607         3860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2404675,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000607 DATNOI010000000
VERSION     DATNOI010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2404675"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VN968_24595"
     CDS             complement(<1..859)
                     /locus_tag="VN968_24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HXL32445.1"
                     /translation="MAKPKSFLRVFTESGPLIALAVAVALLVGIVGPASAQFFNFGGP
                     PQRPQPRSNGGFGGGFGGGGGWFGGDLFAPFQQQAPKQRVREDFSKAPAPDKRETAPE
                     RNVLVLGDAMADWLAYGLEDAYAEQPDMGVIRRHKTGSGLIKYQPKGDPADWAAAAKG
                     ILATEKPDAIVVMLGLNDRVSMREPVAEKSDAKSTDKKSDKKDARTKPDAKPADAKPG
                     AKPDGAADAAAKPEDKADTELSPDDAADNDAPPAIAPEKSTRSPNGVYEFREERWVEL
                     YVKKIEEMIG"
     gene            complement(1029..2459)
                     /locus_tag="VN968_24600"
     CDS             complement(1029..2459)
                     /locus_tag="VN968_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HXL32446.1"
                     /translation="MAQRTEPLAKKRTSAVIIAAALLLCGEARAQSSGGSGLQNFLDN
                     IFTGSIAKGNQTAPSAQRPGASAQAQATPDTGSAPPPWSGEDGASGHPLMTASAIREA
                     AANFDQCVASMWPDAARRNISQLSFQRFTAGLEPDLRIMDLMDSQPEFTKAIWDYLDI
                     LVNDNRLAKGREILAKYKPQFDAAEKTYGVDRYAIASIWGIESNYSTMMGDRNVLQST
                     ATLACIGRRQKYFKDEFLSALEILHRGDLRPEQMRGSWAGAFGPTQFMPTAFKRYAVD
                     ADGDGRRDVVDNPADLIASTANNLKKDGWQTGQSWGFEVVLPQGFNYMLADRAKAMTI
                     GQWQQLGLSRAGGQPFPQPADKAYLLAPAGAEGPGFLMLQNFRVIMKYNPAEAYALAI
                     GHFADRLRGGAPFVQSWPRQERVLSRAERLELQQLLAQRGFYRGTPDGQFGGETREAL
                     RGFQASIGAPADGFASSDVLERLRGR"
     gene            2758..3639
                     /gene="galU"
                     /locus_tag="VN968_24605"
     CDS             2758..3639
                     /gene="galU"
                     /locus_tag="VN968_24605"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HXL32447.1"
                     /translation="MKIRKAVFPVAGLGTRVLPATKAMPKEMLTIVDKPLIQYVVDEA
                     KEAGIEHFIFVTGRNKGVIEDHFDRMYELDATLSARGKKAEMEILARDQPEAGAMSFT
                     RQQAPLGLGHAVWCARDIVGNEPFAVILPDELVLNTPGCLKQMIEAASKLGDKSNLIA
                     VEEVPAHLTHQYGICGVGKRHTHGNMFEVDGMVEKPPKGTAPSNLSITGRYILQPEIF
                     KILETLEHGAGGEIQLTDAMIELQKTQSFYGVEFEGERHDCGSKAGFLRANIAFGMAR
                     ADLREDLRAVMQKYLEK"
BASE COUNT          681 a         1229 c         1239 g          711 t
ORIGIN      
        1 cgccgatcat ctcttcgatt ttcttgacgt agagttcgac ccagcgttcc tcgcgaaatt
       61 catagacgcc gttcggcgag cgggtgctct tctccggcgc gatggccggc ggcgcgtcat
      121 tgtcggcggc gtcgtccggg gacaattcgg tatcggcttt gtcctcgggc ttggcggcgg
      181 cgtccgcggc gccatccggc ttcgccccgg gtttcgcgtc cgcaggcttg gcatccggtt
      241 tggtgcgcgc gtctttcttg tcgctcttct tgtcggtcga tttagcgtcg gatttctcgg
      301 ccacaggctc gcgcatcgag acgcggtcgt tgagcccgag catgacgacg atggcgtccg
      361 gcttctcggt ggcgaggatg cctttcgcgg cggcggccca gtcggccgga tcgcccttgg
      421 gctgatactt gatgagcccg gagccggtct tgtgcctgcg gatcacgccc atgtccggct
      481 gctcggcata ggcgtcctcg agaccatagg cgagccagtc ggccatggca tcgccgagca
      541 ccagcacgtt gcgctccggc gcggtctcgc gcttgtccgg cgcgggtgcc ttggaaaagt
      601 cctctcggac ccgctgcttg ggcgcctgct gctggaaggg cgcgaacaga tcgccgccga
      661 accagccacc gccaccgccg aaaccgccac cgaatccacc gttgctgcgc ggctgcggcc
      721 gctgcggcgg tccgccaaaa ttgaaaaact gcgcggatgc cggtccgacg attccaacca
      781 gcagcgcaac cgcgacagcc agcgcgatca gcgggccgct ctcggtgaaa acgcgcaaaa
      841 aggattttgg ctttgccatg cgctcgttcg caatgaatct cggaaggtca taatagcgga
      901 atcgggccgc aagcgggcag atttcgcgcc atgaaccggg catcggccac tcccgtcaag
      961 ggtgtcgcaa aaatgtggtc cacaaggtta cttttggaac cggaatcggc cgggactgga
     1021 aatcgggctt accgccccct gagccgctcc agcacgtcgg aggaggcaaa tccgtccgcg
     1081 ggggccccga tcgaggcctg gaagccgcgc agggcctccc gcgtctctcc gccgaactgg
     1141 ccgtcggggg tgccacggta gaatccgcgc tgggccagaa gctgctgcag ttccagccgc
     1201 tcggcgcgtg acaacaccct ttcctgccgc ggccaggact gcacgaacgg cgcgccgccg
     1261 cgtaggcggt cggcgaaatg gccgatcgcc agcgcatagg cctccgccgg gttgtatttc
     1321 atgatcaccc gaaaattctg cagcatcagg aagcccggcc cttcggcgcc ggcgggcgcc
     1381 agcaggtagg ccttgtccgc cggctgcggg aagggctggc cgccggcccg gctgaggccg
     1441 agttgctgcc attgcccgat cgtcatcgcc ttggcgcgat cggccagcat gtaattgaag
     1501 ccctgcggca gcacgacctc aaagccccag ctctggccgg tctgccagcc gtcctttttc
     1561 aggttgttgg cggtggaggc gatcagatcg gcggggttat cgaccacgtc gcggcggcca
     1621 tcgccgtcgg cgtcgaccgc gtagcgcttg aacgcggtcg gcatgaattg cgtcgggcca
     1681 aacgcgccgg cccaggagcc gcgcatctgt tccgggcgca gatcgccacg gtgcaggatt
     1741 tccagcgccg agaggaactc atccttgaag tatttctggc gacggccgat gcaggccagg
     1801 gtcgcggtgg actgcagcac attgcggtcc cccatcatgg tcgaatagtt cgactcgatg
     1861 ccccaaatcg acgcgatcgc gtagcggtcg acgccatagg ttttttccgc cgcgtcgaac
     1921 tgcggcttgt acttggccag gatctcgcgg cccttggcga ggcgattgtc gttcaccagg
     1981 atgtcgagat aatcccagat tgccttggtg aattcaggtt gcgagtccat caggtccatg
     2041 atgcgcaaat cgggctcaag ccccgcggtg aagcgctgga agctcagttg cgagatgttg
     2101 cgccgtgcgg cgtcgggcca catcgaggcg acgcactgat cgaaattcgc cgccgcttcg
     2161 cggatcgcgc tcgcggtcat caggggatgg ccggaggcgc cgtcctcgcc gctccatggc
     2221 ggtggcgcgc tgccggtgtc gggcgtggct tgcgcctgcg ccgaggcacc gggccgttgt
     2281 gcggacggag cggtctggtt gcccttcgcg attgagccgg tgaagatatt gtcgaggaaa
     2341 ttctgcaatc cgctgccgcc ggacgactgc gcgcgggcct cgccgcagag caacagtgcc
     2401 gcggcaatga tcaccgcgct ggttcgtttc ttcgccaatg gttccgtccg ttgcgccatg
     2461 tcgtacctgc actgctccag atatcctgcg cgcaggacgc cctgtcacgg tttccaatag
     2521 cttaacaaag cgagattttg ttgtcgggaa acccagcggg aaagaaccgc cggcattcgc
     2581 agctcggctc ctctctgacc ctgcccgacg aggggggagg gaacagacct acgcgtggtg
     2641 atttaccctc gcgtgcggaa gcgccgccct caatatacat ccgcctttgt tcgcggctgc
     2701 aaaccggcta ccaacatgca atccagcgca ctctcttaat ggtatcccga cacccccatg
     2761 aaaatccgta aagccgtttt tccggtcgcc ggcctcggca cccgcgtcct gcccgccacc
     2821 aaggcgatgc caaaggaaat gctgaccatc gtcgacaagc cgctgatcca gtatgtggtc
     2881 gacgaggcca aagaagccgg catcgaacat ttcatctttg tcaccggccg caacaagggc
     2941 gtgatcgagg accatttcga ccggatgtac gagctcgacg ccacgcttag cgcgcgcggc
     3001 aagaaggccg agatggagat cctggcgcgc gaccagcccg aggcgggggc gatgagcttc
     3061 acgcggcagc aggcgccgct cggcctcggc catgcggtgt ggtgcgcccg agacatcgtc
     3121 ggcaacgagc cgttcgcggt gatattgccg gacgaactgg tgctgaatac gccgggctgc
     3181 ctcaagcaga tgatcgaggc cgccagcaag ctcggcgata aatccaattt gatcgcggtg
     3241 gaagaagtgc cggcgcatct cacgcaccag tatggcatct gcggcgtcgg caagcgccac
     3301 acgcacggca acatgttcga ggtcgacggc atggtggaga agccgcccaa gggcaccgcg
     3361 ccatcgaatc tgtcgatcac cgggcgctac attctgcagc cggagatctt caagatcctg
     3421 gaaacgctgg aacacggcgc cggcggcgag atccagctca ccgatgcgat gatcgagctt
     3481 caaaaaaccc aaagcttcta tggcgtcgaa ttcgagggcg agcggcacga ttgcggctcc
     3541 aaggccggct tcctccgcgc caatatcgcc ttcggcatgg cgcgcgccga tttgcgcgag
     3601 gacttgcgcg cggtgatgca gaagtatctg gagaagtgat gtcgacggcg cgctcaacca
     3661 cgccgtcatc atccgcgaaa gcggacgatc cagtattccg caagctcagt tatggaatgc
     3721 agcctcgcgg catactagat gcccgccttc gcgggcatga cgcctttgag ggttttgcgt
     3781 gaacgactac gacctgctgc gcgattatct gcagcagcag acgctgcctg aattcgtgct
     3841 gagcttcgac aagatcgagg
//
LOCUS       DATNOI010000608         5255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1547873,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000608 DATNOI010000000
VERSION     DATNOI010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547873"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..728)
                     /locus_tag="VN968_24610"
                     /pseudo
     CDS             complement(1..728)
                     /locus_tag="VN968_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141440.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(875..1354)
                     /locus_tag="VN968_24615"
     CDS             complement(875..1354)
                     /locus_tag="VN968_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXL32448.1"
                     /translation="MNEVWKKPPVSLEAYQNMVGLEVGVSSWHLVDQSRIDLYADVIE
                     DHQFIHVDPERAKRETSYGTTVAHGFLTMSLMSIMSYEVMPVIEGTTMGVNYGFDKLR
                     FISPVRAGSRVRGRFRLAEAKLRKPKELQSRTNVTVEIEGEEKPALVADWIGLIYFA"
     gene            complement(1358..3094)
                     /locus_tag="VN968_24620"
     CDS             complement(1358..3094)
                     /locus_tag="VN968_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXL32449.1"
                     /translation="MTTFQEARAFLLEHRSDYDTAVAEFRWPDPVPFNWALDWFDAEL
                     ARNLQSKDRPALWIVDAGSNRETKLSFEALSRRSNQVANFLRAQGLKRGDHLLLLLGN
                     VIPLWETMLAAMKLGVVVIPATTLLTPDELRDRLDRGRARVVVASQDQIAKFAGLGGD
                     HLVRIVVSASSKHEGWLAYEQAADFPQTFEPDGPTHADDPMLLYFTSGTTAKPKLVRH
                     SQRSYPVGGLSTMYWLGLRPGDVHLNISSPGWAKHAWSCFFAPWNAGATVFVVNQPRF
                     DAKGLLATIGRCGVTTLCAPPTVWRLFIQEKLATFKVSLREVCGAGEPLNPEVIDQVR
                     AAWGLTIRDGYGQTETTALAGNSPGQKVKVGSMGRPLPGYQVQITDIDGQVTNEGEVS
                     LLLGGDRPAGLMRGYQSDDGALSGADGALYRSGDVVFADDEGYLTFVGRSDDVFKSSD
                     YRISPFELESILLEHEAVAEAAVVPSPDPIRLAIPKAYVLLVSGVARSPATALSIFEH
                     LHTRLAPFKRIRRIELVTELPKTISGKIRRVQLRRLEHDNDRDDPLRGQEFREEEFPE
                     LQQVRSAGSPEN"
     gene            complement(3105..3992)
                     /gene="mmsB"
                     /locus_tag="VN968_24625"
     CDS             complement(3105..3992)
                     /gene="mmsB"
                     /locus_tag="VN968_24625"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HXL32450.1"
                     /translation="MASIAFIGLGNMGGPMAANLVKAGDKVVAFDLVEASRNQAKADG
                     AAIADSAAAAVKGADTVITMLPAGKHVLAVWNEVVPSMAKGALIIDCSTIDVESAVAS
                     HALAAKQGIASVDAPVSGGTGGAKGATLTFMAGGEDKAFAAAKPVLEKMGKKIVHCGG
                     AGAGQAAKICNNMILGISMIAVSEAFALAEKLGLSHQALFDVASTSSGQCWSLTSYCP
                     VPGPVPTSPANNDYRPGFASALMVKDLTLAQDAANAAGAATPLGKHAQEIYRAFDAAG
                     HGGVDFSGIIRHVRTLAGK"
     gene            complement(4020..5108)
                     /locus_tag="VN968_24630"
     CDS             complement(4020..5108)
                     /locus_tag="VN968_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXL32451.1"
                     /translation="MTAQAAAVAEPDLIARREGVVGVIRLNRPKAINAVTLQMFRDID
                     KALDAFEADPDVSVILLEGAGERGLCAGGDIRALYESSKVHGDLGKILWREEYILNAR
                     IAKFPKPYVAFMDGIVMGGGVGLSAHSRHRVVTERTRLAMPEVGLGFFPDVGGTWLLV
                     RSPGEIGTYFGLTGQTMNGPDAIHARFADAVVPSGQLEALREALIQVRAGATSDEIKT
                     IIDGFATGETSGPVAAMQSRIDGWFAYNRMQDIVAGLQRDGSELAQSTLNTLNEKSPR
                     GMVVTLKLLRLARASSSLEECLVREYRAALQVFASDDFREGVRAAVIDKHRNPKWSPP
                     GIEDVTPEMVAPYFAAIGADELVFDKTK"
     gene            complement(5105..>5255)
                     /locus_tag="VN968_24635"
     CDS             complement(5105..>5255)
                     /locus_tag="VN968_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL32452.1"
                     /translation="ALQLHGGYGYLSEYGIEKIVRDLRVHQILEGTNEIMRLIVSRKL
                     IEGAR"
BASE COUNT          948 a         1769 c         1563 g          975 t
ORIGIN      
        1 cgaaggagcc tgcgccggcg cccatgatgg tgcgggtcgg cgcgtcttcg ccgagcagat
       61 acagcaccgc cggcgtgatc gcttccggct tcatcagcgc cagcgcctgc ggcggcagca
      121 gctcttccgt catccgcgtc ggtcggcgaa atcgtgttga cgcggatgtc attcttgcgg
      181 ccctcttccg ccagcacatt catcaggccg accatgccgg ttttggccgc gccgtaattg
      241 gcctggccga aatttccgaa caatcccgat gacgacgtgg tgacgacgat gcggccgtaa
      301 ttgcgctcgc gcatgccgtc ccacaccgcc ttgcagcaat agaaggtgcc ggtgagatgg
      361 acgtccagca ccttggcgaa atcagcgatc tccatcttgg cgaataactt gtcgcgcaag
      421 atgccggcat tggcgcagag cagatcgacg ctgccccatt tttccgtggc cttggcgacc
      481 atcgccctga cctgctcgaa gttcgagaca tcggcgccgt cggccatcgc ctcgccgccc
      541 gcttcgcgga tttcctcgac cacggcttcg gccggcgtca gcgagccccc ggtaccgtcg
      601 cgcgcgccgc cgaaatcgtt gaccacgacc ctggcgccgc ggctcgccag ccccagcgcg
      661 tgcgcgcgtc caagcccatt gcccgcgccg gtgacgatgg cgacgcgtcc gtcaaatctg
      721 attgtcatga gtaaaattcc cggatctggt tctgtcgtca tgcccgggga cctgtccccg
      781 acctgattgg ggatgatcca ggcatccatc aaaagaaaaa catttttgat cagtgatgga
      841 ttgccaggtc aagcccggca atgacggttg gtggttacgc aaaataaatc aggccgatcc
      901 agtcggccac cagcgcgggc ttttcctcgc cctcgatttc gacggtgaca ttggtacgcg
      961 actgcagctc cttcggcttg cgcagcttgg cttcggcaag cctgaagcgg ccgcggacgc
     1021 gcgagcctgc gcgcaccggg gaaatgaatc gcagcttatc gaaaccgtaa ttcactccca
     1081 tcgtcgtgcc ttcaatcacc ggcatcacct catacgacat gatgctcatc agcgacatgg
     1141 tcaggaatcc gtgcgccacc gtggtgccat aagaggtctc gcgtttggcg cgctcgggat
     1201 cgacatggat gaactggtga tcctcgatga cgtcggcata gaggtcgatc cggctctgat
     1261 cgaccagatg ccatgacgac accccaacct ccaggccgac catgttctgg taggcttcaa
     1321 gcgaaaccgg tggcttcttc cagacttcgt tcattgctca gttctccggc gatccggccg
     1381 accgcacctg ctgcaattcc ggaaactcct cttcgcggaa ttcctggccg cgcagcgggt
     1441 cgtcgcgatc attgtcgtgc tccagccggc gcaactgcac ccggcggatt ttgccggaga
     1501 tcgtcttcgg caattccgtc accagttcga tgcgccggat gcgcttgaac ggcgccagcc
     1561 gcgtgtgcag gtgctcaaag atcgacaacg cggttgccgg cgagcgcgcc acgcccgaca
     1621 ccagcagcac ataggctttt gggatcgcca gccggatcgg atcgggactg ggaaccaccg
     1681 cagcctcggc gaccgcttca tgctcgagca aaatgctctc cagttcgaac gggctgatgc
     1741 ggtaatcgga cgatttgaag acgtcatcgg aacggccgac gaaggtcaaa tagccctcgt
     1801 cgtcggcaaa caccacgtca ccgctgcgat agagagcgcc atcggcgccg ctcaatgcgc
     1861 cgtcgtcgct ctggtaaccc cgcatcaggc ccgccggcct atcgccaccg agcagcaggc
     1921 tgacctcgcc ctcgttcgtc acctgtccgt cgatatcggt gatctgcacc tgatagcccg
     1981 gcaacggacg gcccatcgat ccgaccttca ccttctgccc cggcgaattc cccgccagtg
     2041 ccgtggtttc ggtctggccg tagccgtcgc ggatcgtcag cccccaggcc gcgcgcacct
     2101 gatcgatcac ttccggattg agcggctcgc cggcaccgca gacctcgcgc agcgatacct
     2161 tgaaggtcgc gagcttctcc tggatgaaca gccgccacac cgtcggcggc gcgcagagcg
     2221 tggtgacgcc gcagcgcccg atggtcgcca aaagtccctt ggcgtcgaag cgcggctgat
     2281 tgaccacgaa caccgtggcg ccggcattcc agggcgcaaa aaagcagctc caggcgtgct
     2341 tggcccagcc cggcgaggag atgttcagat ggacatcgcc gggccgcagc ccgagccagt
     2401 acatcgtcga cagtccgccg acgggatagc tgcgctggct gtgccgcacc agctttggct
     2461 ttgccgtggt gccggaggtg aaatacaaca gcatcggatc gtcggcatgg gtgggcccgt
     2521 ccggctcgaa ggtttgcgga aaatccgctg cctgttcgta ggccagccag ccctcatgct
     2581 ttgacgaggc gctgacgacg atgcgaacga gatggtcgcc gccgaggccg gcaaatttcg
     2641 cgatctgatc ctgcgacgcc accacgaccc tggcgcggcc gcggtcgagc cggtcgcgca
     2701 gttcgtccgg cgtcagcagc gtggtcgcgg ggatcaccac cacgccaagt ttcatcgccg
     2761 ccagcatggt ctcccacaaa ggaatgacgt tgccgagcag cagcaataga tgatcgccgc
     2821 gcttcagccc ctgcgcgcgc aaaaaattcg cgacctgatt ggaacggcgc gatagcgctt
     2881 cgaacgacag ctttgtctcg cggttgctgc cggcgtcgac gatccacagc gccgggcgat
     2941 ccttgctttg cagattgcgc gccagttccg cgtcgaacca gtccagcgcc cagttgaacg
     3001 gcaccggatc cggccagcga aactccgcga cggcggtatc gtagtcggaa cgatgctcga
     3061 gcaggaatgc gcgggcttcc tgaaaggtcg tcatcgatcg cccatcactt ccccgccaat
     3121 gtccggacat gccggataat cccggagaaa tccaccccgc catggccggc ggcatcgaac
     3181 gcccggtaga tttcctgcgc gtgttttccg agcggcgttg cagcacccgc ggcgttggcc
     3241 gcatcctgcg ccaaggtgag gtccttcacc atcagcgccg aggcaaagcc cggtctgtag
     3301 tcgttgttcg cgggcgaggt cggcaccggg cccggcaccg gacaatagga tgtcagcgac
     3361 cagcactgtc ccgacgaggt cgaggcgacg tcgaacagcg cctgatgcga caaaccaagc
     3421 ttctcggcga gcgcaaaggc ttcgctgacc gcgatcatgg aaattcccag gatcatgttg
     3481 ttgcagattt tcgccgcctg gccggcgccg gcgccgccgc aatgcacgat cttcttgccc
     3541 atcttttcca gcacgggctt ggccgctgca aacgctttgt cttcgccgcc ggccatgaag
     3601 gtcaatgtcg cgcccttggc gccgccggtg ccgcccgaca ccggcgcgtc gaccgaggcg
     3661 atgccctgct tcgcggccag cgcatgggaa gccaccgcgc tttcgacgtc gatggtcgaa
     3721 caatcgatga tcagcgcgcc ttttgccatc gacggaacga cttcgttcca caccgcgagg
     3781 acatgcttgc ccgcgggcag catggtgatg acggtgtcgg cgcccttcac cgcggccgcc
     3841 gcgctgtcgg cgatcgcagc tccgtccgcc ttcgcctggt tgcgcgaagc ctccaccagg
     3901 tcgaacgcaa ccaccttgtc gccggccttg accagattgg ccgccatcgg gccgcccatg
     3961 ttgccgaggc cgataaatgc gatgcttgcc atgttcactc ccctccgctc aaggcgcttc
     4021 tattttgttt tatcgaacac cagctcgtcg gcgccgattg ccgcgaagta aggcgcgacc
     4081 atctccggcg tcacatcctc gattcctggc ggcgaccatt ttggattgcg atgcttgtcg
     4141 atcacggccg cgcgcacgcc ttcgcgaaaa tcgtcgctgg cgaacacttg cagcgcggcg
     4201 cgatattccc gcaccagaca ttcttccagc gaggacgacg cccgcgccag ccgcagcagc
     4261 ttcaaggtga ccaccatgcc gcgcggcgac ttctcgttca atgtattgag cgtcgattgc
     4321 gccagctccg aaccatcccg ctgcaggccc gcgacgatat cctgcatccg gttataggca
     4381 aaccagccat cgatgcgcga ttgcatcgcc gccacaggtc ccgacgtctc gccggtcgca
     4441 aatccatcga tgatggtctt gatttcgtcg gatgtcgcac cggcgcgaac ctggatcagc
     4501 gcttcgcgca aggcttccaa ttgaccggac ggcacgaccg cgtccgcgaa gcgggcatgg
     4561 atcgcgtcgg ggccattcat ggtctgcccg gtcaatccaa aataggtgcc gatctcgccg
     4621 ggtgaccgca ccagcagcca ggtgccgccg acatcgggaa agaaaccaag cccgacttcc
     4681 ggcatcgcaa gtctggtgcg ctcggtgacc acgcggtggc ggctgtgcgc ggacagaccg
     4741 acgccgccac ccatcacgat gccgtccatg aaggcgacat agggttttgg aaatttggcg
     4801 atgcgcgcgt tgagaatgta ctcctcacgc cacaggatct tgccgagatc gccgtgaacc
     4861 tttgagcttt catacagcgc gcggatatcg ccgccggcgc acaggccgcg ctcgccggcg
     4921 ccttccagca gaatcacact cacgtcagga tcggcctcga acgcgtcgag cgccttgtcg
     4981 atgtcgcgaa acatctgaag cgtcaccgca ttgatcgcct tcggccggtt gagtcggata
     5041 acgcccacca cgccctcgcg ccgcgcgatc aggtcgggtt cggcaacagc ggcggcttga
     5101 gctgtcatcg cgcgccctcg atcagcttgc gtgacacgat cagccgcatg atttcgttgg
     5161 tgccttccag aatctggtgc acgcgcagat cgcgcacgat tttctcgatg ccgtattcgc
     5221 tgaggtagcc gtagccgcca tgcagttgca gcgcc
//
LOCUS       DATNOI010000609        29157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_664003,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000609 DATNOI010000000
VERSION     DATNOI010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 29157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29157
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664003"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VN968_24640"
     CDS             <1..962
                     /locus_tag="VN968_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXL32453.1"
                     /translation="LGNEAGYHALLDCDALLMLGADFAWRQFYPDKAKIVQVDIDPTH
                     LGQRHPITKGVVGDVKATMASLLPMLVERTDASFRSAYVKRYAKYREAERAKVAVGHD
                     GSIPGTYLTQVISRHAAKDALFTADDGTAAAWAYRHIEANGQRRIFASLLHGTMANGM
                     PSAIGLQKAEPGRQVVCMAGDGGISMLFGDLMTIVAQELPIKIAVYDNGKLGFVEIEQ
                     RAEGMLDTFTKLKNPNFAGVARALGLWGQTVSDADQLEAAVKDWLAQPGPALLHVHVN
                     PMQLVMPPFMQVEPAIGMALYSTRAILHGRGGDVWEMVRENFL"
     gene            1768..2049
                     /locus_tag="VN968_24645"
     CDS             1768..2049
                     /locus_tag="VN968_24645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32454.1"
                     /translation="MSNLAIVAAILPAAAWWWTNPSLLRLQYLTQVRMRSIIPVPTAK
                     HRPTRLIRLSRRPLRTNAISGLMATLQHRGRRCDAVATSPLREQSWSTK"
     gene            2046..2774
                     /locus_tag="VN968_24650"
     CDS             2046..2774
                     /locus_tag="VN968_24650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXL32455.1"
                     /translation="MTKHIVQLPNPDGSLLLRELNHRIKNELTSAIYAVSAKAVRSDS
                     VAVKAALLDVVDLLHQCADVHRALHMPDRGRLTDAAKYLQQLCFSITKYRLDRLAIRV
                     LFSADDLRLDGERCWKLGLMVSELMTNVARHAQFDAGNPELRVELMLAGNVVKCRVSD
                     NGSAPEPIRRGRGLAIIGELANSLGGRVHTSCAADGYSFLLTFPLTEAEQRAAGATHV
                     VLKRRKMRRPQRLEASGTGDLGAG"
     gene            complement(3443..4999)
                     /locus_tag="VN968_24655"
     CDS             complement(3443..4999)
                     /locus_tag="VN968_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXL32456.1"
                     /translation="MSDKPSAQIEKLSLKAEVDALLVELAVDPISYTGGTLVAKTPIT
                     GETVAHVREVDAAGATSAIEKAHAAFLEWRLVPAPKRGELVRLLGEELRSNKRALGRF
                     VSIEAGKIESEGLGEVQEMIDICDYAVGISRQLFGLTIATERHEHRMAETWHPLGVTG
                     IISAFNFPVAVWAWNAALALVCGNSIVWKPSEKTPLTALATEALFGRALKRFKADGGS
                     APDGLSAVLIGGREIGEALVNNARVPLVSATGSTAMGRAVAPKLAQRFARAILELGGN
                     NAAVVTPTADLDLTLRGVAFAAMGTAGQRCTTLRRLFVHESVYDSFVPRLKKVYASVS
                     VGNPLTGNLVGPLIDGLAYESMRRALGEAEVVGAAIVGGDRVTVAGADAAFYVRPALV
                     EIGGQTGPVERETFAPILYVIKYSDFDAVIELQNAVPQGLSSSIFTNDLREAEAFLSA
                     RGADCGIANVNIGPSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRATNTINYGRTLP
                     LAQGVKFDVD"
     gene            complement(5308..6093)
                     /locus_tag="VN968_24660"
     CDS             complement(5308..6093)
                     /locus_tag="VN968_24660"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32457.1"
                     /translation="MTTLKIDQVSRTFPARPGHVPTLALEPIDLVVGNNDFVTVLGPS
                     GCGKSTLLRIVAGLDRPTTGRVMLDGREVAGPGADRGMVFQSYTLFPWLTVRENIAFG
                     LRERGISEAERGRIADGFIHRVGLSGFETHWPKQLSGGMQQRTAIARALANDPKILLL
                     DEPFGALDNQTRVLMQEMLLGIWERDQKTVLFVTHDIEEAIFLGSRVIVMSARPGRIK
                     AEIAVDLPHPRSYRIKTTPEFIRLKERLVEEIRAEALKVAVDA"
     gene            complement(6090..6866)
                     /locus_tag="VN968_24665"
     CDS             complement(6090..6866)
                     /locus_tag="VN968_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL32458.1"
                     /translation="MTRPLDPVTSKQRAAYGLAFFVLFVALWAWATFGGYVSKTFLAN
                     PLTMLKEGFELLTRHGFLFDIGMTIWRVVGGFVLAAIIAVPLGVLMGAYKPIEAFLEP
                     FVSFARYLPASAFIPLLILWAGIGELQKLLVIFIGSVFQIILMVTVTVGNTRRDLFEA
                     AYTLGAGDRGIIGRVLLPSSAPEIAEILRLVLGWAWTYVIVAELIGSSSGIGHMITDS
                     QALLNTGQIIFGIIVIGLIGLVSDFLFKAFNAWLFPWKLA"
     gene            complement(6952..7896)
                     /locus_tag="VN968_24670"
     CDS             complement(6952..7896)
                     /locus_tag="VN968_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32459.1"
                     /translation="MRNFGIFAATAALLVSAPALAEDTKVAIGISGWTGFAPLTLASQ
                     AGIFKKNGLDVTIKKIPQKDRHLAVASGDIQCAATTVETWISWNANGVATKQIFQLDK
                     SYGADGMATRNDVAAIKDLKGKTIAASAPGTSPYFALAWMLKKNGLAIKDVTVVNLEP
                     AAAAQAFVAGQNDAAMTYEPYLSTVRAAPDKGKIIATTLDYPMVMDTFGCTPKFLTDN
                     PKAAQALADSYFEAVAVIEKDQAKSYEIMGADVKQSGEQFGNSAKYLRWQDKAANQKF
                     FAGEFLAFNKEAAELLLEVGIIKEIPKIDDLYDASFIK"
     gene            complement(8016..8759)
                     /gene="hutC"
                     /locus_tag="VN968_24675"
     CDS             complement(8016..8759)
                     /gene="hutC"
                     /locus_tag="VN968_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HXL32460.1"
                     /translation="MSLTTDRDKLRAGDKPTLYKQIRLDIERRILTGEWPPGHRIPFE
                     HELMAHYGCSRMTVSKALSELAQADLIERRRRAGTFVCRPKFLSAVLKIPDIRAEIGA
                     LGRSYGYELIRCARRAANAADRARLGVRKTGEVIALACRHSADKVPFAIEDRLIDLDA
                     VPEAATADFATEPPGSWLLHHVPWTEAEHSISAIVANHQAAKALDIAIGAPCLVIERH
                     TWRSARPLTAVRLVYPGESHKLVARFKGS"
     gene            complement(8756..10114)
                     /locus_tag="VN968_24680"
     CDS             complement(8756..10114)
                     /locus_tag="VN968_24680"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HXL32461.1"
                     /translation="MSTLHFGSALLPSGWADDVQVVVKDGVITKVMTGVTPGSHNERH
                     QLAIPGIASLHSHAFQRGMAGLAEIRGDSADTFWTWRETMYRFALEMTPEDTEAVATL
                     LYVEMLEQGYTRVGEFHYLHHERDGAPYANPAEMAMRIARAAEITGIGLTLLPSFYAH
                     GSFGGTAPHAGQRRFICSVDQFAKLMAATRQAVRALRGANVGVAPHSLRAVTPDELAA
                     ITPLAEAGPVHIHAAEQVREVDECIAWSGRRPVQWLLEHTPIDQRWCLIHATHMTATE
                     TTSLARSGAVAGLCPVTEASLGDGIFPAREFLNAGGRLGVGTDSNVLIGVTGELRQLE
                     NGQRLKHRERNVLSGGPGVSTGRALFDAALAGGAQALAQPMVGLQAGARADIVTLDTT
                     HPSLAGRRGDAVLDGWIFASAAGAVDCVWAGGSKVVENGHHRLRQKAREAFNAAMRRL
                     VA"
     gene            10208..11422
                     /gene="hutI"
                     /locus_tag="VN968_24685"
     CDS             10208..11422
                     /gene="hutI"
                     /locus_tag="VN968_24685"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HXL32462.1"
                     /translation="MAERFDRIWHNARLATLREDLPELGAIEHGVIAARDGRIVFAGP
                     HCDFPSSADAAERIDCAGRWITPGLVDCHTHLVFGGNRAHEFELQLQGASYEAIARAG
                     GGIVSTVAATRAASEVELVTSALPRLDVLIGEGVTTLEIKSGYGLNTATEMRQLSAAR
                     VLGRTRPVAIRTTFLGAHALPPEADGDKDRYIDLVCQEMLPAVAQAGLADAVDAFMEG
                     IAFSGDQTARVFRAAKALGLPVKLHADQLSNLGGAALASKFLALSADHLEHTDEAGVA
                     AMARAGTVAVLLPGAFYFIRETKRPPVELLRARSVNMALATDCNPGSSPLTSLLLATN
                     MGATLFRMTVAECLAGVTREAARALGVLRETGTLETGKYCDLAIWDIDRPAELVYRIG
                     FNPLYGRVWRGR"
     gene            11419..12996
                     /gene="hutH"
                     /locus_tag="VN968_24690"
     CDS             11419..12996
                     /gene="hutH"
                     /locus_tag="VN968_24690"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HXL32463.1"
                     /translation="MSDNDASIVVSPGKVSLYDLAQVLAGAAVVLDPSFWPRVEAASA
                     IVAEAAQAEAPAYGINTGFGKLASRRIPPDQTARLQRNLIVSHCCGVGPPTPEPIVRL
                     MMALKIVSLGRGASGVRREVIGQLQAMLARGIYPLVPQQGSVGASGDLAPLAHMTAVM
                     IGEGQAFVDGKALPGREALAAADLAPLTLGPKEGLALINGTQFSTAYAISGLWRAHRL
                     ACAALVTGALSVDAAMASTAPFRSEIQQLRGHAGQIVAGAVLMALLEGSDIRMSHLEG
                     DERVQDPYCLRCQPQVAGAALDLLTQAARTLTIEANAVTDNPLVLVETGEIVSGGNFH
                     AEPVAFAADQIALALSEIGAISERRIATLVDPALNFGLPPFLTPDPGLNSGFMIAEVT
                     AAALYAENKQRAAACSIDSTPTSANQEDHVSMAAHAARRLSDMADNLATIVGIELLVA
                     AQGIGLRAPHSTSPALAAVIALLREQVPALGQDRYMADDLAKATALVEADALPTTAIS
                     VLKNSPFPLFAEKGPLS"
     gene            12993..14663
                     /gene="hutU"
                     /locus_tag="VN968_24695"
     CDS             12993..14663
                     /gene="hutU"
                     /locus_tag="VN968_24695"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HXL32464.1"
                     /translation="MNRRLDNDRTIRAPRGPEISAKSWLTEAPLRMLMNNLDPDVAER
                     PSELVVYGGIGRAARDWDSFDRIVASLRKLDDDQTLVVQSGKPVGIFRTHPDAPRVLI
                     ANSNLVPHWATLDHFNELDRKGLMMFGQMTAGSWIYIGSQGIVQGTYETFVEVGRRHY
                     GGNLAGRWILTAGLGGMGGAQPLAATMAGASMLAIECQPSRIEMRLRTGYLDRQASTL
                     DEALAIMAQAALSKKPVSVGLLGNAAEVFPELVRRGVRPDIVTDQTSAHDPINGYLPM
                     GWTLAEWELRRSADPKAVEAAAKTSMVGHVQAMLDFHARGIPTLDYGNNIRQMAKDTG
                     LNNAFDFPGFVPAYIRPLFCRGVGPFRWAALSGDPEDIFRTDARVKELMPDDKQLHNW
                     LDMAKARIKFQGLPARICWVGLGDRHRLGLAFNEMVASGELKAPVVIGRDHLDSGSVA
                     SPNRETEAMRDGSDAVSDWPLLNALLNCASGATWVSLHHGGGVGIGYSQHAGMVIVAD
                     GTPEAARRIERVLWNDPASGVMRHADAGYETAIACARASRLNLPSLTS"
     gene            14861..15853
                     /locus_tag="VN968_24700"
     CDS             14861..15853
                     /locus_tag="VN968_24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017991374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HXL32465.1"
                     /translation="MPPIHIAYLNRLAIEELKMTDDEILSAIETSLASQGRGETVIEP
                     RVHLEPGVANGHFNVLRGAIKPPIDSAGVKIVGDFVDNYKIGFPSELAILALFDPRTG
                     AQKAILDASGITDMRTGAVTAVGAKHLARKNSKILGHIGARGTAYWNVRLLNHLFDFD
                     EIRVHSRREESRNAFAERLSQDLGKPVVATSDWESCVKDADIVVEASRLLEPAPLLKT
                     GWIKQGAFVVPYGTMSAIELSLTDIMAKLVVDDWGQCKFGSLRAHVEAGKLSEKTLHA
                     ELGQIVAGLKPGRESDAETNLLWHRGLSLSDIALGHAMLEKAGKLGVGQRLRFA"
     gene            15875..16690
                     /locus_tag="VN968_24705"
     CDS             15875..16690
                     /locus_tag="VN968_24705"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HXL32466.1"
                     /translation="MTTYVANARMYSVNPEAAAAFRTLFGWLSRRSGVQLDVIDHSFP
                     APLSELWSRADIACVFMCGFPFLDAAPRPKPVAAPQPRGTPGVGRPIYATRLVVRRDS
                     PYQRLEDTFGGRLGYTAEDSHSGFNALRHHLLPYRLQRGANLYRESVGPLYTPRRVVD
                     AVIAGTIDVGPLDSYALDLMLRHEPELERRIRVVSTTDAAPIPFLVAAPQCPDEIVTV
                     LRAALMGFGEATECAAPRDSLCLHGFAPVSVADYDVMANWDQEARAAGFALPT"
     gene            complement(16745..17785)
                     /locus_tag="VN968_24710"
     CDS             complement(16745..17785)
                     /locus_tag="VN968_24710"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32467.1"
                     /translation="MRLTRYKVATAVIIAAFCPNSAIATAQQTVRIGQATSSLSFVSI
                     FAARALKTFDPQDLIATTAVLPGGDPAALAALDAGDIDLAAVGPEAVLRAAAKGQPFE
                     IVYSLMSKVTPQLVVSSAFLDRTGVKAADPLARRLAALKGAIVGVSAIGGTQETVARW
                     LAAKGGLDPKADIKVAQVGGPPALQAALENKRIDAFVLSPPEGYLAEKSGAGTVLVSI
                     SDDFPQVARQPYLVLVAKKPIKEATSDLITRTIKALQAASIALTDNPQPTARTIQAQF
                     FPKADPDGVLAAVKAMNSGIAEGGKLDVAGFQNLLTFSAEVGTDFGKSFDAKTSEGNL
                     WTNHFVDVAKSK"
     gene            complement(17807..18562)
                     /locus_tag="VN968_24715"
     CDS             complement(17807..18562)
                     /locus_tag="VN968_24715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL32468.1"
                     /translation="MIGLLPGIALIVFWQWAAGRLIKEIYISKPTAVAQRLYEIFASK
                     EIYPHLWTTGQELVLGYVIGVALGVIGGYALGRSPRMAGVLEPYVMAFYGIPKIALAP
                     LFIIWFGIGIRSKVALASIMVFFLVFYNVFSGVRGIDQELVNLTRVMGANERQLTRHV
                     YLPAAAPYVMLGMRMAIPYSVIGVIVGEFTSSIQGLGLFIHEASATYDPAGVFAGITI
                     LLVFVIAANLLANRLERRLLRWRTPSGARPNEI"
     gene            complement(18672..19466)
                     /locus_tag="VN968_24720"
     CDS             complement(18672..19466)
                     /locus_tag="VN968_24720"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32469.1"
                     /translation="MDGPVIAFDGIGQDFAAPGRNVPLRVLDHISFGVDGGKFTAVIG
                     PSGCGKSTLLQMAAGLLAPTRGLVRHRGRAVASVNRDVGFVPQQPQLFPWKTLSENVE
                     LPLLLRGVALAERRRRVAAALESVGLEGFEHHYPSQLSGGMQKRGSIARTLVYRPDVI
                     LMDEPFGALDAQTRMVMQHDLQSLSFEAGATVVFVTHDITEAVLLADNVVVLSQRPSR
                     LLTNIAIEIPRPRNVFEPFRNAGFESAYESVWSAFRSQVDIRHRLH"
     gene            complement(19964..20989)
                     /locus_tag="VN968_24725"
     CDS             complement(19964..20989)
                     /locus_tag="VN968_24725"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXL32470.1"
                     /translation="MLNTKQPVLRRFWYAVMPVDLLKDGPRPFTLLGEPIVLFLDANG
                     EPAALEDRCCHRTARLSKGWCKNGNIVCGYHGWEYDRDGTLVMIPQFPFEQPIPDAKA
                     RSFRAQTRYGYIWVALDEPLSDIPDVPEDRDVAYRRIHQFYDKWNTAALRLMENSFDN
                     AHFAFVHKGTFGDINQPKPEKYEIVETDYGFDAETIITIRNPPNASRITGTTDPTTKR
                     HQRNKWFMPFCRRLDMEYPSGIRHIIFNCATPVSDGQIQVVQILFRNDTEANCSTQEL
                     IDWDAAIIAEDRDMLESTDPDAIVDMSRKIEKHMPSDRPGMIMRKRLLELLQSHNEEE
                     QPRQEIA"
     gene            complement(21005..22366)
                     /locus_tag="VN968_24730"
     CDS             complement(21005..22366)
                     /locus_tag="VN968_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL32471.1"
                     /translation="MNSLLIENATGIFSGRPGAAMRTNGAIRIRDGAIVEIGALQAEP
                     NERRLDCHGCVVYPGLISTHHHMFQSVMKGVQAGINLPLAGWLRSVPYTFWSKIDEEA
                     LAVAARIALSEMLLSGTTTVADHHYLFSDSFRFDPAQVIFDVARSLGIRLVFCRGGAT
                     KGRHFDTDEFVPMPVESLDHMLKSVEACAQRFHDTSPGSLSQVVLAPTTPTWSIDPGE
                     LKEAIAAARRMKLRLHSHLSESSEYVDFCLSVHGKRPVEWLADHDWLGPDVWFAHLVH
                     LDLNEVRILAGTGTGMAHCPQSNCRLGSGVAPADAMARLGGAVSLGVDGAASNESADM
                     ISEMHSAWHTHRAVKGADAVTAEDVVHWATAGGARVLGLPQIGTLAPGQQADIAVFNL
                     DQPRHFGMHDPMIAPVTCAGSASIRYLLIGGRMVVENGAIPGLDIQKLRSDAARVVAH
                     LAA"
     gene            complement(22363..23163)
                     /locus_tag="VN968_24735"
     CDS             complement(22363..23163)
                     /locus_tag="VN968_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL32472.1"
                     /translation="MKPLLQSEAFVRIAAPLAVGVILIGVWEVGCRVASIPVYLFPKP
                     SDIFHSLVHNGPSLLRALIVTLRVTLQAFAAAVILGTLIAFIFVQSRAVEVSLFPYAV
                     LLQVTPVVAIAPLIIILVKNTQLSLTICATLVALFPIISNTTLGLRSVDPGLANYFRM
                     NRAGRLKTLIKLRIPGALPYFFGGLRISSGLALIGAVVAEFVAGTGGRSSGLAYEILD
                     AGFTLDIPRMFAALFLITLTGVGLFGAMVALSKLALSNWHESEIGAEA"
     gene            complement(23160..24014)
                     /locus_tag="VN968_24740"
     CDS             complement(23160..24014)
                     /locus_tag="VN968_24740"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32473.1"
                     /translation="MNALLPGEPLDEGPDRMTANGDRPPAVELLSAEKVFANGTQGLA
                     PIDLSIADGEFLTLIGPSGCGKSTLLKLIANLIEPSDGRLLWWRGEFGRVGQDGRRLA
                     FVFQEPTLMPWARVDANVRLPLELANVANAKTAPAVAEAIERVGLSKFWRHFPRQLSG
                     GMKMRVSIARALVTNPNLLLMDEPFGALDEFTRNKLDADLVRLWWERKLTTVFVTHSI
                     YEAVFLSTRIIVMAANPGRIFRTMTIDEPQPRDEQFRSSTKFAAYCRELSTWLAEASL
                     PSASGGAS"
     gene            complement(24023..25012)
                     /locus_tag="VN968_24745"
     CDS             complement(24023..25012)
                     /locus_tag="VN968_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32474.1"
                     /translation="MKSLPRIVATALIGTGVLLGSLAPAPAADKVTFLTSWFAQAEHG
                     GFYQAKATGLYDKAGLDVSIKMGGPQVNGSQLLLAGETDFMMGYDIQVLKGREQSLPL
                     VTVASSFQFDLQGLMTHDDVADIAALKGKPILIAGSSRITFWPWLRAKYGFTDDQIRP
                     YTFNLQPFFADKNVAQQGYPSSEPYQAQQQNEKVKFFLLADGGYPPYGSTIVTTEKMV
                     AEKPDVVARFVKASLEGWRDYIKNPAPANALIKADNPKMTDGQIDFAIEKLKQNKAID
                     GGDAATLGIGVITADRYKQIYDFLVAAELLDPKTDWQKAFDLRFVKDLKIGVQ"
     gene            25356..25748
                     /locus_tag="VN968_24750"
     CDS             25356..25748
                     /locus_tag="VN968_24750"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXL32475.1"
                     /translation="MRALSPLSIKPPFARYSHGIAVPAGHRLVFTSGQLGISADETIP
                     WDCEAQADLCFANIAAILAEDHMTLADVVRINAYVTDREYMQGYMRSRDRQFQGTPPA
                     STLMIVTGFTKPEFLVEIEVVAAAPERD"
     gene            complement(25807..26394)
                     /locus_tag="VN968_24755"
     CDS             complement(25807..26394)
                     /locus_tag="VN968_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXL32476.1"
                     /translation="MMRVLYVYCHPLDDSFHAAIRREALAGLKQAGHTTDLLDLYAEG
                     FNPVLTAERRRDYHDPVRNRANNQAYVDRLTAANALVVQFPTWSFGPPAMLKGWFDRM
                     FGPGIAMDLSDPSQAKPLLLHIKCITGISTYGRPRWQAIAMADPPRKIIKRYLPWFTG
                     SNARVRYHALYHMNTATPEKRSGFLAKVRREMERF"
     gene            complement(26391..27746)
                     /locus_tag="VN968_24760"
     CDS             complement(26391..27746)
                     /locus_tag="VN968_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXL32477.1"
                     /translation="MNDLSSGTLALLIADLGDMDIVTDPKIVRRRSRDFFWYSPILNQ
                     QLDGKSADLIVAPRNEAEVVRVAAACARHRVPLTVRAGGTGNYGQAVPLEGGVLLDVT
                     ALAAVEWIKPGMMRIGAGAKMNAIDAELQPLGQELRMHPSTKRTATIGGFVAGGSGGI
                     GSVNYGGLREPGNILAARVVTVEEQPRIIELRADAAQKINRTYGTTGIITALEMPLAP
                     AWRWIDVVVVFDDFIQAAAFGYEAALADGIVKKLLSPITAPLPSYFGALKPHCPDGKS
                     ILIAMIAEPSLESFKSLLGGRGTMTLETPTDDGPGQVPLYEYTWNHTTLQVLKADRSV
                     TYLQCLYPHDRLMEKVAEMREMFPDEVLQHLEFIRFNGRVTCSGLPVVRYKNADRLNE
                     IIRLHEERGVYIANPHVFTVEDGSRYKRVEADQLGFKHEVDPYGLLNPGKMRSFAKME
                     P"
     gene            complement(27754..28575)
                     /locus_tag="VN968_24765"
     CDS             complement(27754..28575)
                     /locus_tag="VN968_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HXL32478.1"
                     /translation="MAGAPAFTKSGEEQLRPLGSSANNRWAVSETRANLVRPPLTPRP
                     VTVQAEGKLLTIDLARTAIIVIDMQNDFCHPDGWLGHIGVDVGPIRAPIAPLQILLPA
                     LRRQDVPVIWLNWGNRPDRLNLSPALLHVYKPSGAGIGLGDPLPGSAARVLERGSWSA
                     AIVDELTADPKDIQVAKYRMSGFQDTELDSILRNLGVSTLMFAGVNADQCVLCTLQDA
                     NFRGYDCLLLEDCTATTSPDYCMAATIYNIRQCFGFIIRSGSLTRELTSTDRASG"
     gene            complement(28578..28997)
                     /locus_tag="VN968_24770"
     CDS             complement(28578..28997)
                     /locus_tag="VN968_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL32479.1"
                     /translation="MAFSVADISQAKAFRISPADTNYFVMLFDKDTDKIDNIFVIEIF
                     KSGGATPPNEHATAHEFFHVLHGEGVARCGGKTLSIRKGDSLLLHPGSEHVIENTGAD
                     KLYTLTVMTPNEGFAELIRGGDPVDLDADDIRILQGL"
BASE COUNT         5617 a         8686 c         9176 g         5678 t
ORIGIN      
        1 tgctcggcaa cgaggccggc taccacgcct tgctggattg cgatgcgttg ttgatgctgg
       61 gcgcagattt tgcctggcgg caattttacc cagacaaggc aaagatcgtg caggtcgata
      121 ttgacccgac ccatctggga caacgtcatc cgatcacgaa gggcgttgtc ggcgatgtga
      181 aggcgacgat ggcgtcgttg ctaccgatgc tggtcgagcg caccgacgcc tcgttccgaa
      241 gcgcatacgt caagcgctac gcgaagtatc gggaggcgga acgggctaaa gtcgctgtcg
      301 gccacgatgg cagcattccc ggaacctatc tgacacaggt gatcagccgc cacgcggcca
      361 aagatgcgtt gttcacggcc gacgacggta ccgcggccgc gtgggcctat cgccatatcg
      421 aggcgaacgg tcagcgccgc attttcgcga gtctgcttca tggaacgatg gccaacggca
      481 tgccgtcagc cattggactc cagaaggcgg aacccggccg acaagtggtg tgcatggcag
      541 gcgacggtgg aatttccatg ctgttcggag acctgatgac tatcgtcgca caggagctac
      601 caatcaagat tgccgtctat gacaacggca agcttggctt tgtggagatc gaacaaaggg
      661 ccgaggggat gctcgatacc ttcacgaagt tgaagaaccc gaattttgca ggggttgcgc
      721 gcgcgttagg tctctggggc cagacggttt cggacgcgga ccagctcgaa gctgctgtga
      781 aggactggct agcgcaacca gggcctgcgc ttctgcacgt gcacgtcaac ccgatgcagc
      841 tcgtcatgcc gccattcatg caggtagagc cggcgatcgg gatggcgctc tattcgactc
      901 gcgccatctt gcacggccgg ggaggcgacg tctgggaaat ggtgagggag aatttcctct
      961 agccgaggcg ttgctttgcc tgacagaagc gcagcgcccc ggcggcgcag aagtttggga
     1021 atcgatgttc tcgcgccgca cttgcggagc tgtatcgcgg tcatgggcga gcctaaattg
     1081 ctcaatttgc aggagccgta ggcggtgccc gtagcccgct attttatctt cgtcggaggc
     1141 gcgcttgcgg ctctgctgtt gatcgccggc tggtgtctgc caacgccccc agcgatgttc
     1201 gccaatcagc tagccatcga tagatcgatc atccggatca agtctgcgcg caaatggccg
     1261 gaaaaggtcg tcctggacac cagccagccg accataacac cttcggccgt cgaggagcct
     1321 ccggcggcgc agtcggtcgg gctaccgcct gatgaagcgg gagatcaatc gaatctcgag
     1381 gccttggccc agttgaagcc agatacaccg tcggccgctg tcgatcatcc aaccttgcgc
     1441 atcaagcgcg gtttggcaag caccgctcga tcaaagcgtg tggccagagg tcccgttaca
     1501 cgtcggctag caagagcgga agcggggggc tgttgtcagt ttgggtggat cgacaatggt
     1561 caagcaagct cgaatcctat gtcgcgcaag cacgccgcgt cgtcatggcc tatggattgg
     1621 ccggcgctca ccgggaggaa ttgaaagata aacagcggct gtcgttgcac acggaactgc
     1681 gcagttaccg tctaaatgga tcaggaatga acaagatcgt ccgaagtacg tccgctactc
     1741 catggagcat caagaggagc aacgtacatg tccaatcttg caatcgttgc agcgattctg
     1801 cctgctgccg cttggtggtg gaccaacccg tcgttgctca ggctgcaata tctgacccag
     1861 gtgcgtatgc gttctatcat cccggtgccg actgctaaac acaggccgac ccgccttatt
     1921 cgactgagcc gtcgccccct gcgaacgaac gcgatttcag gattgatggc cacgctccaa
     1981 caccgcggtc gtcgttgcga cgccgtcgcc acttctccgc tgagagaaca atcgtggagc
     2041 acgaaatgac aaaacacatt gtacagttac cgaatcccga tggatcattg cttcttcgcg
     2101 agttgaacca cagaataaaa aacgagctca cttccgccat atatgcggtt tcagccaaag
     2161 cggtgcggtc ggatagcgtg gcggtcaagg ccgcgctcct cgacgtcgta gacctcctgc
     2221 atcaatgcgc cgatgttcat cgggcgctgc acatgccgga tcgggggcgc ctcaccgatg
     2281 ccgcgaagta cctccagcag ctttgcttct ccataacgaa ataccgcctg gaccgcctgg
     2341 cgatacgtgt cttgttttca gcggatgatc tgcggcttga tggagaacgc tgctggaagc
     2401 ttgggctgat ggtatccgag ctaatgacca atgtggcacg acatgcgcaa ttcgacgctg
     2461 gaaatccgga attgcgagtg gagttgatgc ttgccggcaa cgtcgtaaag tgcagggtct
     2521 ctgacaatgg ctcggcacct gaacccatcc ggcgcggtcg agggctagcg atcatcggcg
     2581 aactggccaa cagtttgggc ggccgcgttc atacgtcttg cgccgccgac ggatattcct
     2641 tccttctcac cttcccattg actgaggccg agcaacgcgc cgccggcgct actcatgttg
     2701 ttttgaagcg gaggaagatg cgccgccctc agcggttgga ggcgtctgga accggggatc
     2761 tgggggctgg ctaagccacg tggcgttttt cggatcgaag ttgcctgaag aacattcatg
     2821 aaaagcatgg cctgtggatg ggcgagagcg agcatccgcg gctcgacgat ccgagttctt
     2881 gcagcagcgc tcagcttcgc cgtcatgttg ccgctataga gtccggcatg ggatggactt
     2941 atcccggcag gttccttcat cgattagctc ccgccagctg gcagtgaccg ggcctcggct
     3001 ggcgggcctg cccgagggct gagaagaacg gacgctcagt ctgcagctac tgtcagcgga
     3061 tagctgcata accagcggag tagggatgca aaattcacca tggagcgaag cttggccgac
     3121 tgtataagtc cttgctgcat caatctcagc tctgttgaac gcatttcatc gcacgtgcct
     3181 ctcgctgagg actaacatct ttgccgatcg ggctgaagga ccggatttag ggttttcatg
     3241 ccgatcacat gcaacatggc gaggttgaaa ctgtgaccca acaagaaatt cgtgtcggaa
     3301 tcgtgggtgc ggacaacaag gcgagttggg cgaaggtgtc gcacgttccg gcgatcaaag
     3361 gccgacaccc cgatcgacga aagccgggag cccgaccgga acagcgatgc ttgaaaaggg
     3421 gcgcgttcac gacgacttcc attcaatcta cgtcgaactt gacgccttgg gccagcggca
     3481 gcgtccggcc ataattgatt gtgttggtgg cgcggcgcat ataggccttc cacgcatccg
     3541 atcccgactc acgcccgccg cccgtttcct tctcgccgcc gaacgcgccg ccgatttcgg
     3601 cgcccgaagg cccgatgttc acgttcgcga tcccgcaatc ggcgccgcgc gccgacagga
     3661 aggcctcggc ctcgcgcaga tcgttggtga agatcgaaga cgacaggccc tgcggaacgg
     3721 cattttgcaa ttcgatcacc gcatcgaagt cgctgtattt gatgacatag aggatcggcg
     3781 caaaagtctc gcgttcgacc ggtccggtct gtccgccgat ctcgaccagc gccgggcgga
     3841 cgtagaaggc cgcatccgcg cctgcgacgg tgacgcggtc gccgccgacg atggcagcac
     3901 ccacgacctc ggcttcgccg agggcgcgcc gcatcgattc gtaagcgagg ccgtcaatga
     3961 gagggccgac cagattgccg gtgaggggat tgccgaccga gacggaggcg tagaccttct
     4021 tcaggcgcgg cacgaagctg tcatagacgc tctcgtgaac gaaaagtcgg cgcagggtgg
     4081 tgcagcgctg gccggccgtg cccattgccg caaacgcaac gccgcgcagc gtcaggtcga
     4141 gatcggccgt cggtgtgacc accgccgcgt tgttgccgcc gagctccaga atggcgcggg
     4201 cgaagcgttg cgccagcttc ggagcgacgg cgcgtcccat tgcggtcgaa ccggtcgccg
     4261 agaccaaggg aaccctggcg ttgttgacga gggcctcacc gatctcacgt cctccgatca
     4321 acacggctga caacccgtcg ggtgccgagc cgccatcggc cttgaaacgc ttcagggcgc
     4381 gcccgaacag cgcttccgtc gccaaagcgg taagaggcgt cttctccgac ggtttccaga
     4441 cgatgctgtt gccgcagacc agtgccagcg ctgcattcca tgcccagacc gcgaccggga
     4501 aattgaacgc cgagatgatg ccggtgacgc cgagcggatg ccaggtctcc gccatgcggt
     4561 gctcgtggcg ctcggtggcg atcgtcaggc cgaagagctg ccgggaaatt cccacggcgt
     4621 agtcgcaaat gtcgatcatc tcctggactt cgccgagacc ttcggactcg atcttgccgg
     4681 cctcgatgga gacgaaccgg ccaagcgcgc gcttgttgct gcgcagctct tcgccaagca
     4741 gccgcacgag ttcgccgcgc ttgggagcag ggaccagacg ccattcgagg aaggccgcat
     4801 gagccttttc gatggccgag gtcgcaccgg cggcatcgac ctccctcaca tgcgccacgg
     4861 tctcgccggt gatcggggtt ttcgccacca gcgtgccacc ggtatagctg atggggtcga
     4921 cagcgagctc aaccaaaagt gcgtcgacct cggccttgag cgaaagcttt tcgatctggg
     4981 cggatggctt gtctgacatg agggattctt tccgaggact cgcgttgatt tcagagggtc
     5041 tgctgtcagc gattggcttg agccgtcacg atcgttctcc gtcaggaact ggccttgaag
     5101 gcgatgtccc acctcatgga gaagacaatg atccggcatt ttcaaacgat agaagttcaa
     5161 taagtcattc caatgctgaa tgaccagggg gccgagccca aaggatgtgg tggcgaacga
     5221 ctatggccgg tggcagcctg cgccagctct cggtctgccg ggctaacgac gcatgacggg
     5281 aaggccggat cgatgagcca acggccatca cgcgtcaacc gcaaccttta gcgcctcggc
     5341 gcggatctct tcgacgagac gttccttcag cctgatgaat tcgggcgtgg tcttgatcct
     5401 gtaggaccga gggtgaggaa gatcgaccgc gatctcggcc ttgatgcggc cgggacgggc
     5461 gctcatgacg atgacccggc tgccgaggaa gatcgcctct tcgatatcgt gggtgacgaa
     5521 cagcacggtc ttctggtcgc gttcccagat gccgagcagc atttcctgca tcagtacgcg
     5581 ggtctgatta tccagtgccc cgaacggctc gtcgagcaac aggatctttg gatcattggc
     5641 aagcgcacgc gcgatggctg tgcgttgctg catgccgccg gagagctgct tcggccagtg
     5701 agtctcaaag cctgataaac cgacccgatg gatgaaccca tcggcgatcc tgccgcgttc
     5761 ggcttcggaa atcccgcgct cgcgcaggcc gaacgcgatg ttttcgcgca ccgtcagcca
     5821 ggggaacagg gtgtacgatt gaaacaccat accgcggtcc gccccaggtc ccgcgacttc
     5881 gcggccgtcg agcatgacgc ggccggtcgt cggccggtca aggcctgcga caatgcgcag
     5941 cagcgtggat tttccacagc cggaagggcc gaggacggtg acgaagtcgt tgttgccgac
     6001 gacaaggtcg atcggttcca gagcgagcgt cggcacatgg cccgggcgcg cagggaacgt
     6061 gcgggagacc tgatcgatct tcagcgtggt catgccagtt tccagggaaa cagccaggcg
     6121 ttgaaggcct tgaacaggaa gtcagacacg aggccgatca gcccgatgac gatgatgccg
     6181 aagatgatct ggccggtgtt gagcagcgcc tggctgtcgg tgatcatgtg accgataccc
     6241 gaggatgagc cgatcagctc ggcgacgatg acataggtcc acgcccaacc cagaacgagc
     6301 cgaaggattt ccgcgatctc gggggcggag gagggcagca gcacgcggcc gatgatgccg
     6361 cggtcgccgg cgcccagcgt gtaggctgct tcaaagagat cacgcctggt gttgccgact
     6421 gtcacggtga ccatcaggat gatctggaat accgagccga tgaaaatgac caggagcttc
     6481 tgcagttcgc cgatgcctgc ccagaggatc aacagcggga tgaaggctga ggcgggcagg
     6541 taacgggcaa acgagacgaa cggctcgagg aaggcctcga tcggcttgta ggcgcccatc
     6601 agaacgccaa gagggacagc aatgatggcc gccagcacga agccgccgac gacccgccag
     6661 atcgtcatgc cgatgtcgaa caggaagccg tgccttgtca ggagttcaaa gccttccttc
     6721 aacatggtca gcgggttggc gagaaacgtt ttcgagacat agccgccgaa ggttgcccag
     6781 gcccaaaggg caacgaacag gacgaaaaac gcaaggccgt aagccgcgcg ctgcttcgat
     6841 gtcacggggt ccaggggacg tgtcacaggg caaatacctc agagcagcgg gttgatccgg
     6901 tcagtcccgc ttccgcaggt gagaagcggg actgaacggc gccggccgaa cctacttgat
     6961 gaagctcgcg tcatagaggt catcgatttt cggaatttcc ttgatgatgc cgacctcgag
     7021 caacaattcc gcagcttcct tgttgaaagc caggaattcg ccggcgaaga atttctggtt
     7081 cgcggccttg tcctgccagc gcagatattt tgccgagttg ccgaactgct cgccggactg
     7141 cttcacatcg gcccccatga tctcatagga ttttgcttga tccttttcga tcacggcgac
     7201 cgcctcgaaa tagctgtcgg ccagcgcctg tgcggctttc gggttatcgg tcaagaattt
     7261 cggcgtgcaa ccaaacgtgt ccatgaccat cgggtagtcg agcgtggtgg cgatgatctt
     7321 gcccttgtcc ggcgcggcgc gcaccgttga gaggtacggc tcatacgtca tggctgcatc
     7381 gttctgaccg gccacgaagg cctgcgccgc cgccgccggc tccaggttca cgaccgtgac
     7441 atccttgatc gcaaggccgt ttttcttgag catccaggcc agggcgaaat agggcgacgt
     7501 gccaggtgcg gacgcagcga tcgtcttgcc cttcaaatcc ttgatcgccg cgacatcgtt
     7561 tcgcgttgcc atgccgtcag caccatagct cttgtcgagc tgaaagatct gcttggtcgc
     7621 gacgccattg gcgttccagg agatccaggt ttcgaccgtg gtcgctgcac actgaatgtc
     7681 acccgacgcg acggccagat gccggtcctt ctgtggaatc ttcttgatgg tcacgtcgag
     7741 accgttcttc ttgaagatac cggcttggct tgcaagcgta agcggcgcaa agccggtcca
     7801 cccggagatc ccgatggcga ccttggtgtc ttccgccaag gccggcgcgg aaaccaggag
     7861 agcggcggtt gccgcgaata tcccaaaatt acgcatggct gtatccctct tgctggttga
     7921 tggattaccg tcctgttggt cggttcatac ccggtcaatg aagctgctgc attggcggct
     7981 attgtgctgg actcttacgc gcatgggtat ccctctcagc tgcctttgaa gcgggcaacc
     8041 agcttgtggg actcgccggg atagacgagg cgcacagccg tcagcggccg cgcgctgcgc
     8101 caggtgtgcc gctcgatcac aaggcaaggc gcgccgatcg caatgtccaa ggccttcgcg
     8161 gcctgatggt tggccacgat ggcgctgatc gaatgctcag cttccgtcca agggacatgg
     8221 tgaagcagcc acgagccggg cggctcggtc gcaaaatccg ccgtcgccgc ttcgggcacg
     8281 gcatcgagat cgatcagtcg gtcctcgatc gcaaacggca ctttatcggc gctgtgccgg
     8341 cacgcgagcg cgattacctc gccggttttc cgtaccccaa gacgggcgcg atcggcggcg
     8401 ttggcggctc ggcgcgcgca ccggatcaac tcatagccat agctgcgacc gagcgcgccg
     8461 atctccgcgc ggatgtcggg gatcttgagc accgctgaca ggaatttggg acggcacaca
     8521 aaggtgccag cccgccgtcg tcgctcgata agatcggctt gcgccagttc tgacagcgcc
     8581 ttgctgaccg tcatgcgcga gcagccgtaa tgcgccatga gttcgtgctc gaacgggatg
     8641 cggtgacccg ggggccattc accggtcaga atgcggcgct cgatgtcgag acggatctgc
     8701 ttgtacagcg tcggtttgtc gcctgcgcgg agtttgtcgc gatcggtggt gaggctcatg
     8761 ccacgagcct tcgcattgcc gcgttgaatg cttcgcgtgc cttctggcgc agccggtgat
     8821 ggccgttctc gacgaccttg cttccgcccg cccagacaca atcgacggcg cctgcggcgc
     8881 ttgcgaaaat ccagccgtcg agcacggcat ccccgcgacg cccggcaagc gagggatgcg
     8941 tggtgtccag cgtcacgata tcagcgcgtg cgccggcctg aaggccgacc atcggctgcg
     9001 cgagcgcctg ggcgccgccg gcgagcgcag cgtcgaatag cgcgcgaccg gtcgagacgc
     9061 cgggaccgcc ggatagcaca ttgcgttcac ggtgttttag ccgctggcca ttttcgagct
     9121 gacgtagttc accggtgacg ccgatcagga cattggaatc ggtgccgacg ccgagccgtc
     9181 cgcctgcatt aaggaattcg cgggccggaa agatgccgtc gccgaggctt gcttcggtga
     9241 ccgggcagag tccggcgacg gcgccgcttc tggcaagtga ggtggtttcc gtcgcggtca
     9301 tgtgggtcgc atggatgaga caccagcgct gatcgatcgg agtatgttcg agcagccact
     9361 gcaccggccg ccggcccgac caggcgatgc attcatcgac ttccctgacc tgttcggcgg
     9421 cgtggatgtg aaccggcccg gcttcggcca gtggtgtgat ggccgccagt tcgtccggcg
     9481 tcacggcccg caggctgtgc ggcgcgacac cgacattggc ccctcgcaac gcgcggacgg
     9541 cttgacgcgt ggcggccatc agcttggcaa actggtcgac cgagcagatg aaccggcgct
     9601 ggccggcatg cggtgccgtt ccaccaaacg agccatgcgc gtagaagctc ggcagcagcg
     9661 tgagcccgat gccggtgatc tcggcggccc gcgcaatccg catcgccatc tccgctgggt
     9721 tggcgtaggg cgcgccgtcg cgctcgtgat gcagatagtg aaattcgccg acgcgcgtat
     9781 acccttgctc gagcatctcg acatagagca atgtcgcgac ggcttcggtg tcctccggag
     9841 tcatctccag cgcgaagcga tacatcgttt cgcgccatgt ccagaaagta tccgcggaat
     9901 caccgcggat ttcggcaagt cccgccatgc cgcgctggaa cgcgtgacta tgcaggctgg
     9961 ctattccggg gatcgccagt tgatggcgtt cgttgtggga tcccggcgtt acgccagtca
    10021 tcaccttggt gatcacccca tccttcacga ccacctggac gtcgtcggcc catcccgagg
    10081 ggagcagtgc tgatccgaaa tgcagagtgg acatgcttcc aaaccggctg gacagaacac
    10141 caaggtgatt ttatgtatag acataacgga acccccgtca agcttccgtc gctccaggga
    10201 gtgctgcatg gccgagcgct ttgatcgtat ctggcacaat gcccggctcg ccacgctgcg
    10261 ggaggatctg cccgagctcg gtgcgatcga gcacggcgtg atcgcggcgc gcgatggccg
    10321 catcgtgttc gctgggcccc attgcgactt tccgtcgagc gccgatgcgg ccgaacggat
    10381 cgattgtgcg gggcggtgga tcacacccgg attggtggat tgtcatactc acctcgtttt
    10441 cggcggaaac cgcgcgcacg agttcgagct gcagctgcag ggagcaagct acgaggcgat
    10501 tgcacgcgcg ggcggcggca ttgtgtcgac ggtcgctgcg acgcgggccg cgagcgaggt
    10561 tgaacttgtt accagcgcgc tgccgcggct cgacgtattg atcggcgagg gcgtgacgac
    10621 gctggagatc aaatccggct atgggctcaa tacggcaacc gaaatgcgtc agctgtcagc
    10681 cgcgcgtgtg ctgggccgca ctcggccggt cgcgatacga acgacgtttc ttggcgcgca
    10741 cgcgctgcct ccggaggccg atggcgacaa ggatcggtac atcgacctgg tttgccagga
    10801 aatgctgccg gcggtggcgc aggccggcct tgccgacgcc gtcgatgcct tcatggaagg
    10861 cattgcgttc tcgggcgatc aaactgcgcg ggtgttccga gccgcgaagg cgctcggatt
    10921 gccggtcaag ctacatgcgg atcaattgtc gaacctcggc ggcgctgcac ttgcgtcgaa
    10981 attcttggct ctatccgccg atcatctcga gcacacagat gaagctggcg tcgcggcgat
    11041 ggcgcgcgca gggacggtcg ccgtgcttct gccgggcgcg ttctatttca ttcgcgagac
    11101 gaagaggccg ccggtcgaat tgttgcgtgc ccgcagcgtg aacatggcgc tcgcgaccga
    11161 ctgcaacccc ggcagctcgc cgctgacgtc ccttctgctg gccacgaata tgggcgcgac
    11221 gctgttccgc atgaccgttg ctgaatgcct cgccggcgtg acgcgcgaag ctgcgcgcgc
    11281 gctgggcgtt ctccgtgaga ccggaacgct tgagaccggc aaatattgcg acctcgcgat
    11341 ctgggatatc gatcggcccg ccgagctcgt ctaccggatc ggcttcaacc cgttgtacgg
    11401 ccgggtgtgg agaggacgat gagcgataac gatgcgtcga ttgtcgtttc acccggaaag
    11461 gtcagcctct acgatctggc gcaagtgctt gctggcgcgg ctgtggtact cgatccgtcc
    11521 ttctggccgc gcgtggaggc cgcctcggca attgtcgcgg aggccgcgca agcggaagcg
    11581 ccggcttacg gcatcaatac ggggttcggc aaactcgcgt cgagacggat tcctcctgac
    11641 cagacggcgc ggcttcaacg caacctgatt gtttcgcatt gctgcggggt aggaccgccg
    11701 acgcccgaac cgatcgtgcg gttgatgatg gcgctgaaga tcgtctcgct ggggcgaggc
    11761 gcttcaggcg ttcgccgcga ggttatcgga caactgcaag ccatgctcgc gcgcggcatc
    11821 tatccgctgg tgccgcagca gggatcggtg ggagcatccg gtgatttggc gccgcttgcg
    11881 catatgaccg ccgtcatgat cggcgagggg caggcgtttg tcgacggaaa ggccttgccg
    11941 ggccgtgaag cgctggccgc cgccgatctc gcgccgctga cactgggacc gaaggaaggt
    12001 ctcgcgctta ttaatggtac gcagttttcg accgcctatg ccatctccgg attgtggcgc
    12061 gcccaccggc tcgcttgcgc ggcgctggtg acaggcgcct tgtcggtcga cgcggccatg
    12121 gcatcgacgg cgccatttcg ttccgagatc cagcaactgc gggggcacgc cggacagatc
    12181 gtggctggtg ctgtcctcat ggccttgctt gaaggcagcg atatccgcat gtcgcatctc
    12241 gaaggcgacg agcgcgtgca ggatccctat tgcctgcgct gccagccgca ggtggctggc
    12301 gccgcgcttg acctgctgac ccaggccgct cgtaccttga cgattgaggc caacgccgtc
    12361 acggacaatc cgctggtcct ggtcgagacc ggagagattg tgtcgggcgg gaattttcat
    12421 gccgagccgg tcgcattcgc cgccgaccag attgcgctgg cgttgtccga gatcggcgcg
    12481 atcagcgagc gacgcatcgc caccctcgtc gatcccgcgc tgaattttgg cctgccgccg
    12541 ttcctgaccc cagatccggg cctcaactcc ggcttcatga ttgcggaagt gacggcggcc
    12601 gcgctctatg ccgagaacaa gcagcgtgcc gcggcctgct cgatcgattc gacgccgacc
    12661 agcgccaacc aggaagatca tgtgtcgatg gccgcgcatg ctgcgcgccg gctgtccgat
    12721 atggctgaca atcttgccac cattgtgggc atcgaattgc tggttgcggc gcaaggcatc
    12781 gggttgcgtg cgccgcattc gaccagtcca gcgctcgcgg cggtcattgc cttgttgcgc
    12841 gagcaggtgc cggcactcgg tcaagatcgc tacatggctg atgatctcgc taaggcaacc
    12901 gcgcttgtcg aagccgacgc gttgccgacc accgcgattt cggtgcttaa gaacagcccg
    12961 tttccactat ttgccgagaa aggccctttg tcatgaaccg ccgactggac aacgaccgta
    13021 cgatccgggc gccccgtggg cccgagatca gcgccaagag ctggttgacg gaagcgccgc
    13081 tccgcatgct gatgaacaat ctcgatcctg acgtcgccga gcggccgagc gagcttgtcg
    13141 tctacggcgg aatcggccgt gccgcccgcg actgggacag tttcgatcgg attgttgcct
    13201 cactgcgcaa gctcgatgac gaccagacgc tggttgttca gtcgggcaag ccggtcggaa
    13261 ttttccgcac ccatcccgat gcgccgcggg tgctgatcgc caactcgaat ctggttccgc
    13321 actgggcgac actcgatcac ttcaacgaac tcgacaggaa ggggttgatg atgttcggtc
    13381 agatgaccgc cggatcgtgg atctacatcg gtagccaggg catcgtgcag gggacctacg
    13441 aaacgttcgt cgaggtcggc cggcgtcatt acggcggcaa cctcgcaggc aggtggatcc
    13501 tgaccgcggg tcttggcggc atgggcggtg cccagccgct ggccgcgacg atggccggcg
    13561 cgtcgatgct tgcgatcgaa tgtcagccga gccgcatcga gatgcggttg cgcacgggct
    13621 acctcgaccg gcaggcgagc acgctcgacg aagcgctcgc gatcatggcg caagcggcgc
    13681 tgagcaagaa accggtctcc gtcggcctgc tcggcaatgc cgccgaagtt tttcccgagt
    13741 tggtgcgccg tggcgtgagg ccggacatcg tcaccgacca gaccagcgcg cacgatccga
    13801 tcaacggcta tttgccgatg ggatggacgc ttgccgagtg ggagttaaga cgcagcgccg
    13861 atccgaaagc ggttgaagcg gcggccaaga cttccatggt tggccacgtc caggccatgc
    13921 tggatttcca cgcgcggggc attccgacgc tcgactatgg caacaacatc cgccagatgg
    13981 cgaaggacac gggcctgaac aacgcgttcg atttccccgg cttcgtcccg gcctacatcc
    14041 gtccgctgtt ctgccgcggc gttgggccat tccggtgggc ggcgctgtca ggagatcccg
    14101 aggatatctt ccgcaccgac gccagggtga aggagctgat gccggacgac aagcagcttc
    14161 acaactggct cgacatggcg aaagcgcgga tcaagttcca gggattgccc gcgcggatct
    14221 gctgggtcgg cctcggcgat cgccaccggc tcggcctggc gttcaatgaa atggtggcga
    14281 gcggtgaatt gaaggcgcca gtcgtcatcg gccgcgatca tctcgacagc ggctcggtgg
    14341 cgagcccgaa ccgcgaaacc gaggcgatgc gcgacggctc ggatgcggtg tccgactggc
    14401 cgctgctcaa tgcgctgctc aactgcgcca gcggcgccac ctgggtctcg ctgcatcacg
    14461 gcggcggcgt cgggatcggc tattcgcagc acgccggcat ggtgattgtc gccgacggaa
    14521 cgccggaggc tgcgcgccgc atcgagcggg tgctgtggaa cgatcctgcg tctggggtca
    14581 tgcgccatgc cgacgcgggc tatgaaactg cgatcgcgtg cgcgcgcgcc agcaggctca
    14641 atctgccaag cctgacctcg tagcgcgacg gcgcgccgtc agatcgatga tcacgcccga
    14701 tctcatcatt gggtatgcgc cagcgtctgg ctctcgacgg ccattcgcgt tgacttcgat
    14761 tgacgagtga cgttatgtat agacataaaa atttgggcgg ccgggcgagg tcgatcgttg
    14821 cgccaggcct ccggcgaatc ccgggcaagg aggctcgaaa ttgccgccga tccatatcgc
    14881 ctatctcaat cgcctcgcta tcgaagagct caagatgacc gacgacgaaa tcctgtcggc
    14941 catcgagacg agccttgcca gtcagggaag gggcgagacg gtgatcgaac ctcgcgtcca
    15001 tctcgagccg ggagttgcaa acggccattt caatgttctg cgcggcgcga tcaagccgcc
    15061 gatcgactcc gcgggcgtca agatcgttgg cgacttcgtc gacaattaca aaatcggctt
    15121 tccgtccgag cttgcgatcc tggcgctgtt cgatccgcgc accggcgcgc agaaggcgat
    15181 cctggatgcg agcggtatta ccgacatgcg taccggcgcc gtgacggcgg tcggcgccaa
    15241 acatttggcg aggaagaatt ccaaaattct cggtcatatc ggtgcccgcg ggacagctta
    15301 ctggaacgtg cgtcttctca accatctgtt cgatttcgac gagatccgcg tgcattcgcg
    15361 ccgcgaggag agccgcaacg cctttgcgga gcgattgagc caggatctcg gcaagcccgt
    15421 ggtggcgacc agcgattggg agagctgcgt caaggatgcc gacatcgtgg tagaggcgtc
    15481 acgtctgctc gaaccggcac cgctgctgaa gaccggctgg atcaagcaag gcgcattcgt
    15541 ggtcccttac ggaaccatga gtgcgatcga actgtcgttg accgacatca tggcgaagct
    15601 cgtggtcgac gactggggtc agtgcaagtt cggcagcctg cgcgcccatg tcgaggcggg
    15661 taagctgtct gaaaagacgc tccatgccga gcttggacag atcgtcgccg gtttgaagcc
    15721 cggccgcgaa agcgacgcgg agacgaacct gctctggcat cgcggtctct cgttgtccga
    15781 tatcgcgttg ggacatgcga tgctggaaaa ggccgggaag cttggggtgg gacagcgtct
    15841 acgttttgct taggcgcttt caggacgcgg tgtcatgacc acatacgttg caaacgcgcg
    15901 gatgtattcg gtcaatcccg aggcggctgc ggccttcagg accctgttcg gctggctgtc
    15961 gcggcggagc ggcgtgcaac tcgatgtcat cgaccactct tttccggctc cgctctccga
    16021 actgtggtcg cgcgcggaca tcgcctgcgt ctttatgtgc ggatttccgt tcctggacgc
    16081 ggcgcctcga ccgaagccgg tcgccgcgcc gcagccgcgt ggcacgcccg gtgtcggtcg
    16141 cccgatttat gcgacgcgcc tcgttgtccg gagggattcg ccctatcagc gtctcgaaga
    16201 tacctttggc ggtcgcctcg gctacaccgc cgaggactcc cattccggtt tcaatgcgct
    16261 gcgtcatcac ctgctgccgt atcgtctgca gcgaggcgca aacctctatc gcgagagcgt
    16321 cggtccgctc tacaccccgc gtcgcgtggt cgatgccgtc attgccggca ccatcgatgt
    16381 cgggccgctc gatagctatg cgctcgacct gatgttgcgc cacgaaccgg agcttgagcg
    16441 gcgcatccgg gtggtgtcga caacggatgc cgcgccgatt ccgtttctgg tggcggctcc
    16501 gcagtgtcct gacgagatcg tgacggtgct gcgggccgcg ctgatgggat tcggcgaggc
    16561 gacagaatgt gccgctccaa gggatagtct ctgtcttcac ggtttcgcgc cggtcagtgt
    16621 tgccgactac gacgtgatgg caaactggga ccaggaggcg agggcggcgg ggtttgcgtt
    16681 gccgacctga ttcatctccg ccccggatgg ggtcaattcg agtcctcgac gccagtcgtc
    16741 tcggttattt ggactttgcg acatccacaa agtgattggt ccagagatta ccctccgacg
    16801 tcttggcgtc aaagctcttg ccgaagtcgg tgccgacctc cgcggagaac gtcagcaggt
    16861 tctggaaacc tgcgacgtca agcttgccac cctcggcgat gccgctgttc atcgccttga
    16921 cggcggcgag gacgccgtca gggtcggcct tcggaaaaaa ctgggcctgg atcgtacggg
    16981 cggtcggttg gggattgtcg gtcaaggcga tactcgcggc ctggagtgcc ttgattgtcc
    17041 tggtgatcag gtccgaagtc gcttccttga tcggcttctt cgccaccagt acgaggtaag
    17101 gctgacgggc gacctgcggg aaatcgtcgc taatcgacac cagcaccgtt ccggcacctg
    17161 atttttccgc cagataacct tccggcggcg agagcacgaa agcatcgatg cgcttgtttt
    17221 cgagcgcagc ctgtagcgca ggaggtccgc cgacctgcgc taccttgatg tcggccttcg
    17281 gatcgagacc tcccttggcc gcaagccaac gcgccacggt ctcctgggtg ccgccgatcg
    17341 cggagacccc cacgatcgcg cccttcaacg cggcgagccg ccttgccaac ggatcggccg
    17401 ccttgacgcc ggtacgatcg aggaaggcgg aggagaccac aagctgcggc gtgactttcg
    17461 acatcagcga atagacgatt tcgaacggct gccccttggc tgccgcgcga agcacggcct
    17521 ccggtccgac cgccgcaaga tcgatgtcgc cggcatcgag ggccgccagc gccgcggggt
    17581 cgccgccggg cagtaccgcg gtcgtggcga tcaggtcctg tggatcgaag gttttgagcg
    17641 cgcgtgccgc gaagatcgag acgaagctca gcgatgatgt agcttgcccg atccgcacgg
    17701 tctgctgtgc agtcgcaata gcagaattcg ggcagaaagc cgcgatgatc acggccgtcg
    17761 cgactttgta ccgggtcaag cgcatgaatt tgtcctcgga aaaatcctag atttcgtttg
    17821 ggcgggcgcc ggagggggtt cgccagcgca gcaggcgacg ctccagccgg ttggcgagaa
    17881 ggttggccgc gatgacgaag accagcaaaa tggtgatgcc ggcgaacact ccggccggat
    17941 cataggttgc cgaggcttca tggatgaaga ggccgaggcc ctggatcgac gaagtaaatt
    18001 cgccgacgat gacaccgatg acgctgtagg ggattgccat gcgcatgccc agcatgacgt
    18061 agggggccgc cgccggcagg tagacgtgcc tggtcagctg gcgttcgttg gctcccatga
    18121 ctcgtgtcag gttcacgagc tcctgatcga tcccgcgcac gccggagaag acgttgtaga
    18181 aaaccaggaa gaagaccatg atcgaggcga gcgctacctt cgaccgtatg ccgatgccga
    18241 accagatgat gaacagcggc gccaacgcga ttttcggaat gccgtaaaac gccatcacgt
    18301 agggctcgag gacaccagcc attcgcggcg aacgaccgag cgcgtagccg ccgatgacgc
    18361 cgagcgccac tccgatgacg taaccgagca ccagttcctg ccccgtcgtc cacaaatgcg
    18421 gatagatttc tttcgacgcg aagatctcgt agagccgctg cgcgaccgcg gtcggcttgc
    18481 tgatgtagat ttccttgatc aggcggccgg ctgcccattg ccagaaaacg atcagcgcaa
    18541 tgcccggcag caggccaatc aagaggtgtc ccaatcgagc aagccacgaa cgccgcccgg
    18601 gcgctagggg ctcagtggcg gacggttcgg aggttgaata ggccgcgttg ttcaccgtga
    18661 tgcgtccgtt ctcagtgcag ccggtggcga atgtcgacct gagagcggaa ggcggaccat
    18721 accgactcat acgccgattc aaagccggca ttacggaaag gctcgaacac gtttcgcggc
    18781 cgcggtattt cgatcgcaat gttggtgagt agtcgcgacg ggcgctggct caggacaacg
    18841 acattgtcgg caagcagaac cgcttcagtg atgtcgtggg tgacaaagac gacggtcgcc
    18901 cccgcctcga acgaaagcga ttgcagatca tgctgcatca ccatgcgcgt ctgcgcatcc
    18961 agcgcgccga atggctcatc catcaggatg acgtcaggcc gatagaccag cgtgcgcgca
    19021 atcgacccgc gcttctgcat gccaccggaa agctggctcg ggtaatgatg ttcgaaaccc
    19081 tcaaggccga cggattcaag cgctgcagct acccgacggc ggcgctcggc gagcgctacg
    19141 ccgcgtaaca gaagcggcaa ttccacattt tctgacagcg tcttccaggg gaagagttga
    19201 ggctgctgcg gaacgaagcc gacgtcgcgg ttgacgctgg ccacggccct tccacgatgg
    19261 cgcacgaggc cgcgtgtcgg cgcgagcaat cccgccgcca tctgcaacag cgtgctcttt
    19321 ccgcagccgc tcggcccaat gacggcagtg aacttgccgc catcgacgcc aaagctgatg
    19381 tggtcgagca cacgtaaggg gacgttgcgc ccgggggcag caaaatcctg cccgatgcca
    19441 tcaaatgcga ttacaggacc gtccatcatc ggagctcccg gggacaatta cgtatataca
    19501 taattggacg ctcgatgtcc aacaagctta gatggtgggc atagcctgca tctttcaggc
    19561 gcatgcgagg ccagatcctc acgagttgtg accgaactat cgtggcccag cggaggcggt
    19621 ttcacaaaca gagtcgggca gggctgaaca cgatcggaga tcgcgtagca tgcggcgcca
    19681 tctgtacgca gttcgggaaa cacctcaaca gaacgtgagc gacatgatga aattggatca
    19741 cacagccgga ccggtctgga acgtgtcgga tcaagagcgc ccgacatggt ccgtggcgcc
    19801 catgatggat tggaccgaga gttcaagtgt ttcaattaat tagaaggcgg catgtgcacg
    19861 atatgtgcac cgagagatca aagggtatcc agagacaacg acggatcagc cgttcgaatt
    19921 cgagccccga ggatctcctt caagcaccaa cacctccttg agctcaggcg atttcttgcc
    19981 gcggttgttc ctcttcattg tggctctgaa gcaattcgag caagcgcttg cgcatgatca
    20041 ttcccggccg atccgacggc atgtgctttt cgatcttccg gctcatgtcc acgatggcat
    20101 cggggtcggt tgattccagc atatcccgat cctcggcgat gatcgcggcg tcccaatcga
    20161 tcagttcctg cgtagagcag ttcgcctcgg tgtcgttgcg aaagaggatt tgaacgactt
    20221 gaatctgacc gtcggatacc ggcgttgcgc aattgaaaat gatgtgccgg atgcccgacg
    20281 ggtattccat atcaagccgc cggcagaatg gcatgaacca tttgttgcgt tggtgtcttt
    20341 tcgttgtggg atcggtcgtt ccagtgattc tggacgcatt gggtggattc ctgatcgtga
    20401 tgatcgtctc ggcgtcaaat ccgtaatcag tctccacgat ttcgtacttc tcaggcttgg
    20461 gctgattgat gtcgccgaag gttcccttgt gcacaaaggc gaaatgggca ttgtcgaacg
    20521 aattttccat tagccgaagc gcagccgtgt tccacttgtc gtagaactgg tggattcggc
    20581 ggtaagcgac gtcgcggtct tcgggaacgt cgggaatgtc gctcagcggt tcatcaagcg
    20641 cgacccagat gtaaccatag cgggtctggg ctcgaaacga gcgcgccttg gcgtcgggaa
    20701 ttggttgttc gaatggaaat tgcggaatca tcacgagcgt gccgtcgcgg tcatattccc
    20761 acccgtgata gccgcataca atattgccgt tcttgcacca gcccttcgaa agcctggcgg
    20821 tacggtggca gcagcgatct tcgagtgcgg ccggttcgcc gttggcgtcg aggaacagca
    20881 cgatcggttc accgagcagc gtgaacggcc gtggtccgtc cttgagcaga tcgacgggca
    20941 tcaccgcgta ccagaagcga cgaagcacgg gttgtttggt attcagcatg acgttctccg
    21001 gtggtcaggc agccagatgc gcgacgacgc gggccgcatc ggatcttagt ttttgaatat
    21061 ccaggccggg gattgcgcca ttctccacga ccatccggcc tccgatcagc aaataacgga
    21121 tactggccga gcccgcgcag gtgaccggag cgatcattgg atcgtgcatg ccgaaatgcc
    21181 gtggctgatc cagattgaag acggcgatgt cggcctgctg cccgggcgcc agcgtaccga
    21241 tctgaggcag tcccagcacg cgcgcgccgc cagccgtcgc ccagtgtacg acatcttctg
    21301 cagtcacggc gtcggcacct ttgacagccc gatgcgtatg ccaggcgctg tgcatctcgc
    21361 tgatcatatc ggcggattcg ttggatgccg cgccgtccac gccgagggac actgcgccgc
    21421 cgaggcgagc catcgcgtcg gcgggggcga cgccagagcc gaggcggcag ttggattgcg
    21481 gacaatgcgc catgcctgtt ccggtacctg ccagaatgcg gacttcattg agatccaggt
    21541 ggaccagatg cgcgaaccac acatccggtc caagccaatc atggtcggcc agccattcaa
    21601 ccgggcgctt gccgtggacc gagaggcaaa aatcaacgta ctcggaggat tccgatagat
    21661 ggctgtgcag ccgcaacttc atccgccgcg ccgctgcgat cgcttccttc agttcgcccg
    21721 gatcgataga ccatgtcggc gtggtcggcg ccagcacaac ctgggaaagg ctgcctggcg
    21781 aggtgtcgtg aaagcgctgc gcgcaggctt cgacggactt gagcatgtgg tcgagcgatt
    21841 cgacgggcat cggaacgaat tcatcggtgt cgaagtgacg ccccttggtg gcgccgccgc
    21901 ggcagaatac caagcggatg cctaggctgc gcgcaacatc gaaaatcacc tgcgcgggat
    21961 cgaacctgaa actgtcgctg aagagataat gatgatccgc caccgttgtc gttcccgaca
    22021 gcagcatttc agagagggcg atacgggcgg cgacggccag cgcctcttcg tcgattttcg
    22081 accaaaacgt ataaggcacc gagcgaagcc aaccggcgag tggcagattg atgccggcct
    22141 gcacaccctt catgacgctt tggaacatgt ggtgatgggt cgatatcagg ccgggataaa
    22201 cgacacagcc gtggcaatcg agcctgcgct cattcggctc cgcctgcagc gcaccgattt
    22261 cgacgatcgc gccgtcgcga atgcggatcg cgccgttggt ccgcatggca gctccagggc
    22321 gcccgctgaa aatcccggtc gcattctcga tcagcaagga gttcacgctt ctgctccgat
    22381 ttcgctttcg tgccagttcg acagtgcgag cttcgataaa gcgaccatcg ctccaaacaa
    22441 cccaactcca gtcaaagtga tcaagaacag tgccgcgaac atgcggggaa tatcgagcgt
    22501 gaagccggca tccaggattt cataggctag tccggacgaa cggccgccgg tgcctgccac
    22561 aaattcggcg acgaccgcgc cgatgagcgc taagccgctg gaaatacgca agccgccaaa
    22621 gaaatatggc aacgccccgg gaattctgag cttgatgagg gttttgagcc gccccgcgcg
    22681 attcatacgg aagtaattcg caagacccgg gtcgacgctt ctcagcccga gcgtcgtgtt
    22741 cgagatgatg gggaacagcg ccaccagtgt cgcgcagatc gtcagcgaga gctgggtgtt
    22801 cttgacgaga atgatgatca gcggggcgat ggcgacgacg ggtgtgacct gcagcagcac
    22861 agcgtaagga aacagactta cttcgactgc ccggctctgc acaaagatga acgcaatcag
    22921 cgtaccaaga atgaccgccg ccgcgaacgc ctgcaacgtc acccgcaagg tcacaatcag
    22981 cgctcgtagc aatgacggac cgttgtgtac aaggctgtgg aagatgtcgc tcggctttgg
    23041 aaacaagtaa accgggatcg atgccacccg gcagccaact tcccacacac cgatcaagat
    23101 gacgccgacc gcgagaggtg cggcgatccg gacaaaggct tccgactgca gcagcggctt
    23161 cacgacgcgc ctccggacgc tgagggcagg gatgcttcgg caagccaggt cgatagttcg
    23221 cggcagtaag ccgcaaattt ggtgctactg cgaaattgct cgtcgcgtgg ctgcggttcg
    23281 tcgatcgtca tggtccggaa gatgcggcct gggtttgccg ccatcacgat gatccgcgtc
    23341 gataggaaga cggcctcata tatcgagtgc gtgacgaaca ccgttgtgag ttttcgctcc
    23401 caccataatc gaaccagatc agcgtcaagc ttgttgcggg tgaattcgtc aagcgccccg
    23461 aagggctcat ccatcagcaa caggttgggg ttggtcacaa gcgcccgggc gatagatacc
    23521 cgcattttca tgccgccgga caattggcgc ggaaaatgcc gccaaaattt cgacaacccg
    23581 acacgctcga tcgcctccgc cacggccgga gcagtcttgg cgttcgcaac attggcgagt
    23641 tcaagtggca gccgcacatt ggcatcgact cgcgcccatg gcatcaaggt gggctcctgg
    23701 aacacgaagg caaggcgtcg cccgtcctgg ccgacccttc cgaattcgcc gcgccaccac
    23761 aagagccggc cgtccgacgg ctcgatgaga ttggcgatca atttgagcag cgtactcttg
    23821 ccgcagccgg atgggccgat caaggtcagg aattcgccgt ctgcgatgct cagatcgatc
    23881 ggcgccaacc cctgcgtgcc gttggcgaaa actttctcgg ctgaaagcag ttcgaccgcc
    23941 ggcggccggt cgccgttggc ggtcattcgg tccgggcctt cgtcaagcgg ctcgccaggc
    24001 agaagggcgt tcatggccgc tcctattgca ccccgatttt gagatccttg acgaaacgaa
    24061 gatcgaaggc cttttgccaa tccgtctttg gatcgagcag ttcggctgcg accaggaaat
    24121 cgtagatctg cttgtagcgg tcggcggtga tgaccccgat gcccaaggtc gccgcatccc
    24181 cgccgtcgat ggccttgttc tgcttgagtt tctcgatcgc aaagtcgatt tggccatcgg
    24241 tcattttggg attgtcggcc ttgatcaagg cgttggctgg agccggattc ttgatgtaat
    24301 cgcgccagcc ttccagcgat gccttcacaa agcgtgccac cacgtcgggc ttttcggcga
    24361 ccatcttctc ggttgtgaca atcgtggagc catacggcgg gtagccgcca tcggccagca
    24421 gaaagaattt taccttttca ttctgctgct gcgcctggta cggctcggac gaaggatagc
    24481 cctgctgggc gacattcttg tcggcgaaga acggctggag gttgaaggta tatggccgta
    24541 tctggtcatc ggtgaaacca tatttggcgc gcagccaggg ccagaatgtg atgcgggatg
    24601 agcctgcgat cagaatgggc ttgcctttca acgctgcgat atctgctacg tcatcatgag
    24661 tcatcaaccc ctgcaggtcg aactggaatg aggacgcgac cgtcaccagg ggcaagctct
    24721 gttcgcgccc cttcaggacc tgaatgtcgt agcccatcat gaaatcagtc tcgccggcca
    24781 gcaacagctg cgagccgttg acctgcggac cgcccatctt gatggaaacg tcgagaccag
    24841 ctttgtcata aagtccggtc gctttggcct gatagaaacc cccgtgctcg gcctgggcga
    24901 accagctggt gagaaaggtc actttgtcgg cggctggcgc cggcgcaagc gagccgagca
    24961 atacaccggt tccgatcaag gcagttgcga cgatacgtgg cagagatttc atggccatct
    25021 cggctaacct cgtttcgggc aggcgtttcg ccggccatct gtgttctcgg gatatgcgaa
    25081 tgccgggcca atctcgggca accgatccct ctcgatcgag aaaccatcgt cctgctggcc
    25141 ctgtcatcgc aaagatgcac aggtaacctg tcacctgaaa gcactttaat tcgagcgatc
    25201 tgttgccttt tcggcaacga ttgcggctcg cgtcgggccg actgccgctc caggccttgc
    25261 tcttccaaaa atggccaatg acgtcgttgg gaaaatcgat ttactattcc gctgcgcctt
    25321 ccgttttttg ccttcgcagc atcaggataa ccgctttgcg cgcgctttcg cctttgtcga
    25381 tcaaacctcc ctttgcacgc tacagccacg ggatagcggt gcccgcaggg cataggctgg
    25441 tctttacctc agggcagctt ggcatcagcg cggacgaaac gattccctgg gattgcgagg
    25501 cgcaagctga cctttgcttt gccaatatcg cggcaattct cgcggaagat cacatgacat
    25561 tggcggatgt cgtccgcatc aacgcctacg tgacggatcg tgaatatatg cagggctata
    25621 tgcggtcacg cgaccggcaa tttcagggga ccccgccggc ttcgacccta atgatcgtga
    25681 cgggttttac gaaaccggaa ttcctggtgg aaatcgaggt tgtcgcggcc gcgccggagc
    25741 gtgattgatt cgaacctggg attggcgaga ggaccaagag gccttcttgt cggtatcgaa
    25801 gcggcgctag aagcgctcca tctcccgtcg cacctttgcc aaaaagccgc tccgcttctc
    25861 cggcgtcgcg gtgttcatgt gatagagcgc gtgatagcgc accctggcgt tggaacccgt
    25921 aaaccacggc aggtaccgct tgatgatctt gcgtggcgga tcggccatgg cgatcgcctg
    25981 ccagcgcggg cgtccgtagg tcgagatgcc ggtaatgcat ttgatgtgaa gcagcagcgg
    26041 ctttgcttgt gatggatcgg aaagatccat cgcgatcccc gggccgaaca tccggtcgaa
    26101 ccaacccttg agcatggcgg gcggcccgaa tgaccaggtc gggaattgca cgaccagggc
    26161 attggcggct gtcagccgat cgacataggc ctgattgttg gcccggtttc gcaccgggtc
    26221 gtggtagtcg cggcgtcgtt cggcggtgag aacgggatta aaaccctccg catagaggtc
    26281 gagcagatcc gtcgtgtgcc ccgcttgctt caatcctgcc aatgcttctc tgcggatcgc
    26341 ggcatggaag ctgtcgtcga gcggatggca atagacgtac agcacgcgca tcatggttcc
    26401 attttcgcaa aactgcgcat cttgccggga ttgagcaagc cgtatgggtc gacctcatgc
    26461 ttgaatccca gctggtccgc ttcgacgcgt ttgtagcggc tgccatcttc gaccgtaaag
    26521 acgtgcggat tggcgatata gaccccgcgt tcttcgtgaa gccggatgat ctcgtttagc
    26581 ctatccgcat tcttgtagcg caccactggc agtccgctgc acgtaacgcg gccgttgaag
    26641 cggatgaatt cgagatgctg cagcacttcg tctggaaaca tctcccgcat ctcggcgacc
    26701 ttttccatca agcggtcatg cggatagagg cattgcaggt aggttacgga acgatcagcc
    26761 ttcagcacct gaagggtggt atggttccag gtgtattcat agagcggtac ctggccgggg
    26821 ccatcgtcgg tcggcgtctc cagcgtcatg gtgccgcggc cgccgagcag ggatttgaag
    26881 ctttcgagcg agggctccgc gatcatcgcg atcaggatgc tcttaccgtc aggacaatgt
    26941 ggcttaagcg cgccgaaata cgagggcagc ggggccgtga tcggcgacaa caatttcttg
    27001 acgatgccgt cggcgagcgc cgcttcataa ccgaaggccg cagcctgtat gaaatcgtcg
    27061 aacacgacga cgacgtcgat ccatctccag gcgggcgcta aaggcatttc gagcgcggtg
    27121 atgatccctg tggtgccata ggtgcggttg atcttttgcg ccgcgtctgc gcgcagttcg
    27181 atgatacgcg gctgctcctc gaccgtaacg acgcgcgcgg ccagaatgtt accgggttcg
    27241 cgcaggccgc cgtagttgac cgagccgatc ccgcccgacc cgccggcaac gaatccaccg
    27301 attgtggcgg tccgcttggt cgaaggatgc attcgtaatt cctgccccaa cggctgcagc
    27361 tcggcgtcta tcgcgttcat cttcgcgccg gcgccaatgc gcatcatgcc aggtttgatc
    27421 cattcgacgg ccgcgagtgc cgtgacgtcg agcagtactc cgccttccag cggtaccgcc
    27481 tggccgtaat tgccggtgcc gccggctcgc accgtcagcg gcacccggtg gcgcgcacag
    27541 gctgctgcta cgcgcacgac ttctgcctcg ttgcgcggcg cgacgatcag atcggccgac
    27601 ttgccatcga gttgttggtt caggattggg ctgtaccaga agaaatcgcg cgagcgcctg
    27661 cgcacgatct tgggatcggt gacgatatcc atgtcgccaa ggtcggcgat caacaaagcg
    27721 agcgtgccgg acgacagatc gttcatgacc aggtcatccc gatgcccgat cggtgctggt
    27781 aagttcgcgc gtgagtgacc ccgatctgat gatgaagcca aagcactggc gaatgttgta
    27841 gatggtcgcg gccatgcaat agtccggtga agtggttgcg gtacaatcct caagcaacag
    27901 acagtcatag ccgcgaaaat tggcgtcctg cagcgtgcac agcacgcatt ggtcggcatt
    27961 tacgccggca aacatcagtg tggagacgcc gagattgcgc aggatgctgt cgagttcggt
    28021 atcctggaag ccggacatgc ggtatttcgc gacctggatg tccttgggat cggcggtcag
    28081 ctcatcgacg atcgcggccg accaactgcc gcgttcgagc accctggcgg cggaaccggg
    28141 cagggggtcg ccaagaccta tgccggcgcc cgaaggctta taaacgtgaa gcaacgccgg
    28201 gctgagattc agccggtcgg gtcggttgcc ccagttgagc cagatcaccg gcacatcctg
    28261 cctgcgcaac gcgggcaaca aaatttgcag cggcgcaatg ggcgcgcgga ttgggccgac
    28321 atcgacgccg atatggccga gccagccatc ggggtggcag aagtcgttct gcatgtcgat
    28381 cacgatgatg gcggtacgcg cgaggtcgat cgtcagcagt ttgccttcgg cttgaacagt
    28441 aacaggacgt ggtgtcagcg gcggccgtac cagattcgcc cgcgtctcgg agaccgccca
    28501 gcgattgttt gcgctcgaac cgagcggccg cagctgctcc tcaccgcttt tggtgaacgc
    28561 cggcgcccct gccatttcta gaggccctgc aatattcgga tgtcgtcggc gtcgagatca
    28621 accgggtcgc cgccgcgaat gagctcggca aagccttcat tcggcgtcat caccgtgagc
    28681 gtatagagct tgtcagcccc cgtgttctcg atcacgtgtt ccgaaccggg atgcagcagc
    28741 agcgagtcgc ctttcctgat cgacagcgtc ttgccgccgc agcgcgcgac gccttcgcca
    28801 tgcaggacgt ggaagaattc atgggccgtg gcgtgctcgt tcggcggcgt cgcgccgccc
    28861 gatttgaaga tttctatgac gaagatgttg tcgatcttgt cggtatcctt gtcgaaaagc
    28921 atcacgaaat aattggtatc cgccggcgaa atgcggaatg ccttcgcttg cgaaatatct
    28981 gcgacgctga atgccatcga aagctcctgt cggcctctag tacccccttt tcttagaagg
    29041 tgagtcgtga ttcaagatcg ggatgatacc cgaagcaaga gaagtccacc tttcgaggaa
    29101 agatcgcaag gtgcttgagg cgtgttctcg ctcaccgacg acattgcagc gcgattt
//
LOCUS       DATNOI010000610         2808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_776716,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000610 DATNOI010000000
VERSION     DATNOI010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776716"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VN968_24775"
     CDS             complement(<1..1150)
                     /locus_tag="VN968_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXL32480.1"
                     /translation="MTALPQDVVADLRRRVAELEQTLESALAERDEAIAQQAATAEVL
                     QVINASPGDLAPVFDAIVERAARLCAADGGALWLVDGDTARATGGRGGKMPEAFYDYV
                     EHESVPVKFLLGRDQDRPFVHVVDLKATKPYQDGIPFFVANVELGRVRTSLSVPLKEC
                     GRLIGIFTLIRWQVQPFTDKQIAVVQSFADQAQIAMKNAGLLSELGIAGDRQAASAEI
                     LRAIASTPGDAERPLQQVAETTARLFGASSVTLNIASGGEWVRMIRVGPSSQRVGSEL
                     SAAQLGIGGRNLPATVFRENRQIHIPDLDNLDPAIADWPSLPHARAAGARAMAGTPLR
                     REGKAIGALIVHRDRPTPFSDEELALLQSFADQAVIAIENARLFNETQE"
     gene            complement(1345..>2808)
                     /locus_tag="VN968_24780"
     CDS             complement(1345..>2808)
                     /locus_tag="VN968_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HXL32481.1"
                     /translation="TSGRRIGAFCMQHGPGAENAMGGVAQCYGESVPVLVLPMGYARR
                     LANIEPNFNSSQAMKAFAKSSEPINLAGEVGNIFRRAFSKLKNGRGGPVIVEIPADMW
                     NEEVPEPLNYTPVLRTRYGADPVHVKEAAALLTGAKRPVIYAGQGVHYAQAWPQLKRL
                     AERLAIPVTTSLGGKSSFPETHPLSLGSRGLAVPRAVPKFLGEADVIFGIGCSFTETN
                     FGVAMPKGKTIIHSTLDPAHLNKDVEARVGLVGDAALVLDALLEEIGKTVTSDRDASA
                     VAAEIAGSHKEWLAKWMPKLTSSDAPLNPYRVLWDLQRTVDIRNTIITHDAGSPRDQL
                     SPFWKAVEPLSYIGWGKTTQLGYGLGLAMGAKLAKPDKLCINVWGDAAIGFTGMDFET
                     AVRERIPIMSVLLNNFSMAIELKVMPISTEKYRSTDISGDYAAMARAFGGHGERVTKP
                     EDIVPAIQRGIAKTREGVPVLLEFITSKETEVSRPGT"
BASE COUNT          486 a          901 c          887 g          534 t
ORIGIN      
        1 cctcttgggt ctcgttgaaa agtcgtgcgt tctcgatggc gatgacggcc tgatcggcaa
       61 aactttgcag cagcgcgagc tcctcgtcgc tgaaaggcgt gggccggtcg cgatgcacga
      121 tgagggcgcc gatcgccttg ccctcgcgcc gcagcggcgt gccggccatc gcgcgggcgc
      181 cggcggcgcg cgcgtgtggc agagagggcc agtcggcgat cgccgggtcg aggttgtcca
      241 gatcgggaat gtggatttgc cggttctcgc gaaacaccgt cgcgggcaga ttgcgcccac
      301 cgatcccaag ctgcgccgcc gaaagctctg agccgacacg ttgcgagctg ggccccacgc
      361 ggatcatccg aacccattcg ccaccgctgg cgatattgag ggtgacgctg gatgcaccga
      421 acaggcgcgc cgtggtctcc gcgacctgtt gcagcggccg ctcggcatcg cccggcgtgc
      481 tggcgatagc gcgcaggatt tcggcgctcg cggcctgccg atccccggcg atgcccagct
      541 cgctcaggag ccccgcgttc ttcatcgcaa tttgcgcctg atcggcaaaa ctttgcacga
      601 ctgcgatttg cttgtcggtg aacggctgca cctgccagcg aatgagggtg aatatgccga
      661 tcaggcgtcc acactctttc agaggaacgc taaggctcgt ccgaacccga ccaagctcga
      721 cgttggcgac gaaaaacggg atgccgtcct ggtagggctt ggtcgccttg agatcgacga
      781 cgtggacaaa ggggcggtct tgatcgcggc ccaacaagaa tttgaccggt accgactcgt
      841 gctccacgta gtcgtaaaac gcctccggca tcttgccgcc gcggcccccg gtagcccggg
      901 ccgtatcgcc gtcgacgagc cagagtgcgc caccgtcggc ggcacacagc cgtgcggccc
      961 tctcgacgat cgcatcgaac acaggcgcga ggtcaccggg cgaagcgttg atgacctgca
     1021 atacttccgc agtcgcggcc tgttgggcga tcgcttcgtc gcgctcggcg agagctgact
     1081 cgagcgtctg ctcaagttcg gcaaccctgc gcctgagatc ggccaccacg tcctggggaa
     1141 gcgcggtcat tcactcaaat ccattttgcc ccgcgcctgg cgccgggctc aaataccagc
     1201 atacccgtcc gggccgccgg ttcatacggg ggctggccgg caaggccgtc tgcctccaaa
     1261 aataggtcaa tgtctgatta ttccactcca gacgcatctc tgccagcgat cccggcgttt
     1321 tttcgcgccc ggcggacagc ggccttaggt ccccggtcgt gaaacttcgg tctccttgct
     1381 ggtgatgaat tccagcagca ccggcacgcc ttccctggtc ttggcaatgc cgcgctggat
     1441 ggcggggacg atgtcttccg gctttgttac ccgctcgccg tggccgccga aggcgcgcgc
     1501 catcgcggca taatcgccgg agatatcggt cgagcgatat ttttcggtcg agatcggcat
     1561 caccttcaat tcgatcgcca tgctgaagtt gttcaacagc accgacatga tcgggatgcg
     1621 ctcgcgcacc gcggtctcga aatccatgcc ggtgaagccg attgccgcat cgccccagac
     1681 gttgatgcag agcttgtccg gttttgccag tttggcgccc atcgcgagcc ccagtccata
     1741 gccgagctgg gtagtcttgc cccagccgat ataggacagc ggctcgaccg ccttccagaa
     1801 tggcgagagc tgatcgcgcg ggctgccggc atcgtgggtg atgatggtgt tcctgatgtc
     1861 gacggtgcgc tgcaaatccc agagcacgcg atagggattg agcggcgcgt cactgctggt
     1921 cagcttcggc atccattttg cgagccactc cttatgcgag ccggcaatct cggcggcgac
     1981 ggctgacgca tcgcggtccg aggtgacggt cttgccgatt tcttccagca gcgcgtcgag
     2041 caccagggcg gcatcgccga caagcccgac cctggcctcg acatccttgt tgagatgcgc
     2101 ggggtcgagc gtcgaatgga tgatggtctt gcccttgggc atggcgacgc cgaaatttgt
     2161 ctcggtgaac gagcagccga tgccgaaaat cacgtcggct tcgccaagga atttcggcac
     2221 cgcgcgcggc accgcaagac cgcgtgagcc gagcgaaagc ggatgcgtct ccggaaatga
     2281 cgacttgccc ccgagactgg tggtcacagg gatggcgagc ctctcggcca gccgcttcag
     2341 ttgcggccaa gcttgcgcat aatgcacgcc ctgtccggca tagatcacgg gacgtttggc
     2401 gccggtcaac agcgccgccg cttccttcac atgcacgggg tcggcgccgt aacgtgttcg
     2461 cagcaccggg gtgtaattca gcggctcggg cacttcctca ttccacatgt cggcggggat
     2521 ttccacgatg acaggcccgc cgcggccgtt tttcagtttg ctaaaagcgc ggcgaaaaat
     2581 gttgccgact tcgccggcga gattgatcgg ctcggacgat ttcgcaaacg ccttcatggc
     2641 ctggctggaa ttgaagttcg gctcgatatt ggcaagccgc cgcgcatagc ccatcggcag
     2701 caccaatacg ggcacggatt cgccgtagca ttgcgccacg ccgcccatag cgttttccgc
     2761 gccgggaccg tgctgcatgc agaaggcgcc gatcctgcgc cccgatgt
//
LOCUS       DATNOI010000611        11150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_461614,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000611 DATNOI010000000
VERSION     DATNOI010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461614"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..510
                     /locus_tag="VN968_24785"
     CDS             25..510
                     /locus_tag="VN968_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HXL32482.1"
                     /translation="MRRSLLLAAAVFTTLAASIAGVEAQAPMQRVQVGVLECRGGASI
                     GFIVGSVTNLGCVLRVEGMPEDRYVATIRKVGLDLGITQESALAWGVYAPVARLGPGD
                     LSGNYAGAQGSASVGVGVGGNVLVGGSANSIALQPLSVQGQVGLNVAAGLESLELRPG
                     R"
     gene            624..2420
                     /locus_tag="VN968_24790"
     CDS             624..2420
                     /locus_tag="VN968_24790"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYF domain-containing protein"
                     /protein_id="HXL32483.1"
                     /translation="MAGSWYLFDGARQLGPLSLSELKHFLEVKSLPDVQVWREGLKEW
                     AAPSDLPEFAPVGPPPIPTISEDGRGQGADDDIATKRHRFNNFIARNWRGEFSLGTTY
                     WLFGFLGNLFAGVLAIAVMAAFQSDGGYEPRAIFATILLVWVGIVTIAVWQTVGVWRS
                     ANRHIKARVLLGKKSPWAGLAKLAVCFGALRLAGTFLTSGWPQLLETGRMSFLDDPDI
                     PAYSIRVMRNGTEAEITGGFKYGLTDDFVKILNASRQIKVVHLDSLGGRIGEAEKLNK
                     VIRSKNLDTYVSSKCMSACTVAFAGGARRTLRKGAVLGFHAPSFPGMSRDELAEASTS
                     QKDIFVAAGFDKKFVDQALSTPSSALWKPAFSVLLQAKAITAISDGSDYAMSGLGTSL
                     TKNDFALMLSKALPLMEALKIKFPDDYDSVVQAYYGSFESGKTEAESVAASRAKLLVI
                     VRKLRPLADDGVLADIGAVYADQYQALGSKSLALCYQYASGVGNTVAPSDIPEALIQR
                     ENDINRRVVETALNRAAVNATVSEALWKKVGTVLASKGIRSEQFDLLSTATVPTEKYG
                     DYCTTVTMLFREISKLPQNEAGILMRDVLAAK"
     gene            2564..4612
                     /locus_tag="VN968_24795"
     CDS             2564..4612
                     /locus_tag="VN968_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXL32484.1"
                     /translation="MGQDVRSPRGPRCIALVGPFQSGKTTLLEAILARTGAIPRAGSV
                     DAGTSVGDASPEARHHKMGVGLTAATTSFMGDSYTFIDCPGSVEFAHDMRAAIPAVDA
                     AVVVCEADERKLPQLQLILRELEDLAIPRFLFLNKIDRANKRIRETLATLQPASRIPL
                     VLRQIPIWNGELIAGFVDLALERAFVYREHKASEDVGLEGGDLDREKEARFSMLEKLA
                     DHDDALMEQLLEDIPPPRDAVFDDLARELREGLICPVLLGSAMRENGVLRLMKALRHE
                     SPGVAETAKRLGASSPKDALAYVFKTVHLQHGGKLSLARVLTARLDDGATLQSSSGEA
                     ARVSGISAVNCAHDTKRPSAEAGDTVALGKLDSIKTGDTVSNGKTAPPALVKVEAISP
                     ALAIAIAATDRKDDVKLGQALLRLNEEDPSLTMIQNPRTHDIVLWGQGEMHLRVALER
                     LHDRFGVNVKSQAPAIGYQETIRKSITQRGRHKKQSGGHGQFGDVVLDIAPLPRGTGF
                     EFHEKVVGGAVPRNYIGAVEEGVVDGLTRGPLGFPVVDVQVTLTDGSYHTVDSSDLAF
                     RTAARIGVSEGLPQCQPVLLEPIHVVEIVCPTDATAKINAILSGRRGQILGFDTREGW
                     PAWDRVRAMMPEAEIGELIVELRSATAGAGSFTRQFDRMAEVTGRAADQIIAAHRVAA
                     "
     gene            4736..5662
                     /locus_tag="VN968_24800"
     CDS             4736..5662
                     /locus_tag="VN968_24800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXL32485.1"
                     /translation="MGLARDRLFGAVVTSQTARHHEHCDCDAGARRGIFAARASQLAR
                     SVMNEIDLSLKMRRLAAWLDGRCQPDSLVRKQFHGAPFGSCYVTIDPDRQGPFASANL
                     NRVYLCGTEAGMDFDSVERLIELFATEGVKRFFVWLSPGPNMDEVRRWLQGSGLSRIR
                     RTGYPTLCRSGRSPVQFRTDLDIREVSVDDVEAARDQLGDTLWPEYARSAGTKDFFHY
                     MAFDGERAVAIAALCVFEDLGYLMGAATAESHRKRGAQQALIAKRVERAEQIGCSIMV
                     SETLYMLEHSYRNLQRAGFQEVYEKEVYEWSA"
     gene            5729..6364
                     /locus_tag="VN968_24805"
     CDS             5729..6364
                     /locus_tag="VN968_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HXL32486.1"
                     /translation="MLDSRRNIALACALSALAGYVDGIGFLHLGGLFVSFMSGNSTRM
                     GVSLAEGHWLGAIEALGLVALFVIGAAAGSVIVLGRGANRQPWVLLAEASLLAAGALC
                     YAFGLSNVAVAAIVLAMGLENAVFQIEGGAGLGLTYVTGALVKVGQLAAEALRGGARW
                     GWAPNLLLWAALVAGSLCGAVGYHWINLAAIWFAAASSLALSAMLAVTIHL"
     gene            6456..7298
                     /locus_tag="VN968_24810"
     CDS             6456..7298
                     /locus_tag="VN968_24810"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HXL32487.1"
                     /translation="MTAVTKARAACLIGWPAAHSRSPLIHHYWLRALGIEGGYNIEAV
                     PPEGFAEFVLHLSTHGFVGANVTIPHKERVLALTKPDERARAVGAANTLWYEGAELCS
                     TNTDIEGFIGNLDACAQGWDAAGDALVLGAGGSSRAVVFGLIERGISRVHLANRTLER
                     AQALAHQFGPSVHPVAWDAIGGLLPRAGVLVNTTSLGMHGQPALELDAGLLPSHAVVA
                     DLVYVPLETPLLVAARARGLKTADGLGMLLHQAVRGFELWFGQRPEVTSELRALVEAD
                     LTKV"
     gene            7360..7986
                     /locus_tag="VN968_24815"
     CDS             7360..7986
                     /locus_tag="VN968_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32488.1"
                     /translation="MPVRRSTLIRPLVAVAMVVASALYAIAQQPPSPSRVRGTIEAID
                     GDVLSVKSRSGEDVKLRMTGDMRVVGITRISLSDIKVGSFIGTTTVPGPDGSQDAVEV
                     HVFPEDMRGTGEGSRPYDLRPNSTMTNATVAQTVAGNDGHTLMIKYKDGEKKVVVSPD
                     TPVVTYVPADKSDLKAGAKVIAFMKQLPDGTFETNRVSVGRDGLTPPM"
     gene            8086..8706
                     /locus_tag="VN968_24820"
     CDS             8086..8706
                     /locus_tag="VN968_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32489.1"
                     /translation="MPRALAAAVTLSVLASSVFASVAWAQQPPPVRIRGTIESVDGAM
                     LMIKSREGTDMKVRVTDNVAVFGVAKTEMSEIKPGSYIGVSAMPEPDGTQKALAVHIF
                     PETQRGAAEGFRPWDLRPNSTMTNATVAETVKGTDGQNILVKYKDGEKKVVVPPDTPI
                     VTFVAGDKSELKPGAKIIIFGAVKKDDGTLEANRVNVGRDGITPPM"
     gene            complement(8941..10077)
                     /locus_tag="VN968_24825"
     CDS             complement(8941..10077)
                     /locus_tag="VN968_24825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HXL32490.1"
                     /translation="MKHITCIEDLRQVHMRKVPKAFFDYADRGSYAEETLRANRDDLQ
                     QIKFRQRILVDVSKRDLSTTILGEPAALPLILAPIGLCGMQFGDGEIHACRAAQAAGI
                     PFTLSTMSICSIEDVAENVDKPFWFQLYVMKDRGFIKALIERAIAAKCSALVLTVDLQ
                     VIGQRHADIKNGMTVPPEWSVSKFLDFASKPGWVSGVLRGKHRTFGNLMGHLKGTDDI
                     TALGTWISTQFDTTLNWKDVEWIRSIWPGKLILKGILDVEDAEEAAKTGAQALVVSNH
                     GGRQLDGAPSSIEVLPEIVDVVGSQMEVMFDGGIRSGQDVMRALALGAKSCMIGRAYI
                     HGLGAFGGPGVAKAIDLIANELSVTMGLCGVNTIAEIDDHVLAV"
     gene            10185..10352
                     /locus_tag="VN968_24830"
     CDS             10185..10352
                     /locus_tag="VN968_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32491.1"
                     /translation="MTGPRSDPKDVKGAEKRRLDQALEEGLEETFPASDPVNVTQPPP
                     SKGDHHVKRKD"
     gene            10569..>11150
                     /gene="glmM"
                     /locus_tag="VN968_24835"
     CDS             10569..>11150
                     /gene="glmM"
                     /locus_tag="VN968_24835"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029512.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HXL32492.1"
                     /translation="MSRKYFGTDGIRGRANGLITPELALKVGQAAGLVFQRGEHRHRV
                     VIGKDTRLSGYMIEYAMVAGFTSVGMDVLLLGPMPTPAVAMLTKSMRADLGVMISASH
                     NLFEDNGIKLFGPQGFKLSDDVERQIEQLMDESLDKRLAQSASLGRARRIDGVHDRYI
                     EFAKRTLPRDLSLDGMRVVVDCAHGAAYRVVPEA"
BASE COUNT         2067 a         3361 c         3581 g         2141 t
ORIGIN      
        1 cacattccag ccggagattc cttcatgcgc cgctccctgc ttcttgccgc agccgttttc
       61 accacgctgg cggcctccat tgccggcgtc gaggcgcaag ctccgatgca gcgcgtgcag
      121 gtcggcgtgc tcgagtgccg cggcggtgca agcatcggtt tcattgtcgg ctcggtaacc
      181 aatctcggtt gcgtgctgcg cgtcgagggc atgccggagg accgctatgt cgcgaccatc
      241 cgcaaggtcg gtctcgatct cggcatcacc caggaatcgg cgctggcctg gggcgtctac
      301 gcgccggtgg cgcggctcgg gcccggcgat ctctccggca actacgccgg cgcgcagggc
      361 agcgcgtcgg tcggcgtcgg cgtcggcggt aatgtgctgg tggggggatc ggcgaattcg
      421 atcgcgctgc agccgctcag tgtccagggc caggtcggcc tcaatgtcgc ggccgggctg
      481 gaaagcctgg aacttcggcc gggaaggtag acgctgttcc ccctgccggg tgctcgcggc
      541 agttgccaac agctctccac tgttcgggtg acgagaggac gcgcaacctg tagtatggca
      601 ccggtcgtgc cgggcggggc tctatggcgg gcagttggta ccttttcgac ggggcgaggc
      661 agcttggtcc gctgagcctg agtgaattga agcacttttt ggaagttaag agcttgcctg
      721 acgttcaggt atggcgcgag ggcctgaagg aatgggctgc gccatccgat ttgccggagt
      781 ttgctccggt ggggccgccg cccatcccaa caatttccga agacggacgc ggccagggtg
      841 ccgacgatga catagccacc aagcgacacc gctttaacaa ctttatcgcc aggaactggc
      901 gcggagaatt ttcgctcggc acgacttatt ggctgttcgg ctttttggga aatctattcg
      961 cgggtgtttt ggcaatagcc gtgatggcgg ctttccaatc cgatggaggt tatgagccac
     1021 gagcgatttt tgccacgatc ctcctcgtat gggtcgggat tgtaaccatc gccgtttggc
     1081 aaacggttgg ggtctggaga tccgccaacc gccatatcaa ggctagagtc cttctcggca
     1141 aaaaatctcc ctgggcgggc ctggcgaaac tagccgtgtg tttcggagct cttcgtctgg
     1201 ctggaacatt ccttacgtca ggttggcctc agttattgga gacgggccga atgtcatttt
     1261 tggatgatcc cgacattccg gcctattcaa ttcgggtcat gcgcaacgga accgaagcgg
     1321 aaatcaccgg cggcttcaaa tatggtctca ccgacgattt cgtgaaaata ctaaatgcct
     1381 cacggcaaat aaaagtggtt catctcgaca gcctcggtgg gagaataggc gaagccgaaa
     1441 aactcaataa agtcattcgc agcaagaatt tggacaccta cgtgtcgtcg aagtgcatgt
     1501 cggcctgtac tgtcgcgttt gcaggcggcg cgcgcaggac ccttcgaaag ggcgcggttc
     1561 tcggatttca tgcgccatcg tttcccggaa tgtccagaga tgagcttgca gaagcgtcga
     1621 caagtcaaaa agatattttt gtagccgcgg gcttcgacaa gaaatttgtt gaccaggctc
     1681 tctcgacacc gagttctgcg ctctggaagc cggcgttcag cgtgcttctg caggctaaag
     1741 cgataaccgc aatttcagat ggcagcgatt atgccatgtc gggattaggg acatcgctaa
     1801 ccaagaatga ttttgcatta atgttgtcga aagcgcttcc gctcatggaa gcgctgaaga
     1861 taaaatttcc tgacgactac gattccgtag tgcaagcgta ttatggcagt ttcgagagcg
     1921 gcaaaaccga agcagagtct gttgcggcca gtcgcgcaaa attgcttgtc atcgtcagga
     1981 agctcagacc tcttgcggac gatggcgttc tcgcggatat cggcgctgtg tatgcggacc
     2041 agtatcaggc gctcggttcc aagagcctag cgctttgcta ccaatatgcg tcgggtgtcg
     2101 gcaatacggt tgcgccgtca gacataccgg aggctctcat acagagagag aacgatatca
     2161 acaggcgagt ggtggaaacc gcattgaacc gcgctgccgt caatgcaacg gtgtcggaag
     2221 ctctctggaa gaaagtcgga acagtattgg ctagtaaggg cattagaagt gaacaattcg
     2281 atttgctatc gactgcaacg gtgcctaccg aaaagtacgg agattattgc acgacggtca
     2341 cgatgctctt tagggagatc agcaaactac ctcaaaacga agcggggata cttatgcgtg
     2401 acgttctggc cgccaaatag ccccgccgga acgcaagggg tcaaaactga cgaccgtccc
     2461 cgcaccgcag cgacgttttt ccggcttgcc aaatcgtcca tgtaggggga gcatccagcc
     2521 tgccgaccca atcaagggcc gagctccaga tgaggaggcg gcaatgggac aggacgtcag
     2581 aagtccccgc ggtccacggt gcattgcgct ggtgggccct ttccaaagcg gtaaaaccac
     2641 acttcttgaa gcgattttgg cgcgcacggg cgcaattccc cgcgccggca gcgtggacgc
     2701 cggaacttcg gtcggcgatg ccagccccga ggcccgccac cacaagatgg gcgtcggcct
     2761 caccgccgcc acgacaagtt tcatgggcga cagctacact tttatcgatt gccccggctc
     2821 ggtggagttc gcgcacgaca tgcgcgccgc gatcccggcg gtcgatgccg cggtggtggt
     2881 atgcgaggcc gacgaacgca agctgccgca gcttcagctt atcctgcgcg aactcgaaga
     2941 tctggctatc ccgcgctttc tgtttctcaa caagatcgac cgcgccaaca agcgcatccg
     3001 cgagacgctc gcgacattgc agccggcgtc gcggattccg ctggtgctgc ggcaaatccc
     3061 gatctggaac ggcgagttga ttgcgggttt cgtcgatctg gcgctggaac gcgccttcgt
     3121 ctatcgcgaa cacaaggcgt ccgaagacgt aggcctcgaa ggcggcgatc tcgaccgcga
     3181 gaaggaggcc cgcttctcga tgctggaaaa gctcgccgat cacgacgacg cgctgatgga
     3241 gcagttgctc gaggatattc cgccgccgcg cgacgccgtg ttcgacgatc tcgcccgcga
     3301 attgcgtgaa ggactgatct gtccggtgct gctcggctcc gcgatgcgcg agaacggcgt
     3361 gctgcgcctg atgaaggcgc tgcgccatga atcgcccggt gtggccgaga ccgcaaagcg
     3421 tctcggtgcg tcctcgccca aggatgcgct ggcctatgtg ttcaagaccg ttcatttgca
     3481 gcatggcggc aagctatcgc tcgcccgcgt gcttaccgcg cggctcgacg acggcgccac
     3541 cttgcaatcc tcatcgggcg aggcggcccg ggtgtccggg atatcggcgg tgaattgcgc
     3601 gcatgacacc aagcgtccct ccgccgaagc cggcgacacc gtcgcgctcg gcaagctcga
     3661 cagcatcaag accggcgata ccgtgtcgaa cggcaagacc gccccgccgg cgctggtcaa
     3721 ggtcgaagcg atttcgccgg cgcttgcgat cgcgatcgcg gctaccgacc gcaaggacga
     3781 cgtcaaactc ggccaggccc tgttgcggct gaacgaggag gatccctcgc tgaccatgat
     3841 ccagaacccc cggacccacg acattgtgct gtggggacag ggcgagatgc acctgcgggt
     3901 cgcgctggag cgcctgcacg accgcttcgg cgtcaacgtc aaatcgcagg ccccggcgat
     3961 cgggtaccag gagaccatcc gcaaatcgat cacccagcgt ggccggcaca agaaacaatc
     4021 cggcggtcac ggccagttcg gcgacgtcgt gctggacatc gcgccgctgc cgcgcggtac
     4081 cggcttcgag tttcacgaaa aggtcgtcgg cggcgcggtg ccgcgaaatt atatcggcgc
     4141 ggtggaagag ggcgtggtcg atggactaac gcgcgggccg ctcggctttc ccgtcgtcga
     4201 tgtgcaggtg acgctgacgg atggctcata tcataccgtc gactcttccg atctcgcatt
     4261 ccgcaccgcg gcgcggatcg gcgtcagcga gggactgccg caatgccagc cggtattgct
     4321 ggagccgatc cacgtggtgg agatcgtctg tccgaccgat gccaccgcga agatcaacgc
     4381 gatcctgtcg gggcggcgag ggcagattct cggtttcgac acccgtgaag gctggccggc
     4441 atgggaccgc gtccgcgcca tgatgccgga agccgagatc ggcgagttga tcgtggagtt
     4501 gcggtcggcg acggccggcg ccggcagctt cacccggcag ttcgatcgca tggccgaggt
     4561 caccggccgc gccgccgatc agattatcgc cgcccatcgc gtcgccgctt gattttgcat
     4621 gccgcccttg ctggccgcga attcgcgaca aacttgacgc cttgtgcgtc aagtttgcgc
     4681 cgaaatgcgt catttttgct tgcaattctg tgggacgcct cggcgaacgc cgcaggtggg
     4741 cctcgcgcgt gatcgccttt ttggcgcggt cgttacttcc caaaccgccc gccatcatga
     4801 acactgcgac tgcgacgcgg gcgcgcgtcg tggtattttt gctgcacggg catcgcagct
     4861 cgcgaggagc gtaatgaacg agatcgattt gtcgttgaaa atgcggcggc ttgccgcctg
     4921 gctggacggc agatgccagc ctgacagcct cgttcgaaag caattccatg gcgcgccctt
     4981 cggcagttgt tatgtcacca tcgatcccga ccgccagggc ccgtttgcgt ccgcgaatct
     5041 caatcgcgtc tatctgtgcg gaactgaagc ggggatggat ttcgacagtg tcgaacgctt
     5101 gattgagctg ttcgcgaccg aaggggtcaa gcggttcttc gtctggctca gccccggccc
     5161 caacatggac gaggtgcgcc gctggctgca gggcagcggg ctctcccgta ttcgacgcac
     5221 cggctatccg accctgtgcc gcagcggccg ttcgccggtt caattcagaa ccgatctcga
     5281 catacgcgag gtgagcgtcg acgacgtgga agcagcccgc gatcaactgg gcgataccct
     5341 gtggcccgaa tatgcgaggt ccgcggggac aaaggacttc ttccattaca tggcgttcga
     5401 cggcgaacgt gccgtagcca tcgcggcgct gtgcgtgttc gaggaccttg gatatctgat
     5461 gggggcggcg accgccgaaa gtcatcgcaa gcgcggtgcc caacaggccc tgattgcaaa
     5521 gagggtcgag cgggccgaac agatcggctg ttcgatcatg gtgtccgaaa cgctgtatat
     5581 gctcgagcat tcctaccgca atctgcagcg cgccggcttt caggaagtgt atgaaaaaga
     5641 agtctatgaa tggagcgcct gaagcgtcct cgttgcctcc gccggtcgac accgcggcgc
     5701 cgcatccacc gccaggcggt tcaatgccgt gcttgattca cggcgcaaca ttgccctggc
     5761 ctgtgcgctg agcgcgctgg ccggctatgt cgatggcatc ggcttcctgc atctcggcgg
     5821 cctgttcgtc tccttcatga gcggcaactc gacacggatg ggcgtcagtc ttgcggaggg
     5881 gcattggttg ggcgcgatcg aggcgctcgg cctggtcgcg ctgttcgtga tcggtgccgc
     5941 ggccggcagc gtgattgtgc tcggtcgcgg cgccaatcgc cagccctggg tgctgctggc
     6001 ggaagcgtcg ctgctcgccg ccggggcact gtgctacgca ttcggactgt cgaatgtcgc
     6061 ggtcgccgcc atcgtgctgg cgatgggact ggagaacgcg gtgttccaga tcgagggcgg
     6121 cgccggcctc ggtcttacct acgtcaccgg cgcgctggtg aaggtcggcc agctcgcggc
     6181 cgaggcgctg cgcggcggcg cgcgctgggg ctgggcgcct aacctgctgt tatgggcggc
     6241 gctggtggcg ggttcgttgt gcggtgcggt cggctatcac tggatcaatc tcgccgcgat
     6301 ctggtttgcc gcggcctcgt cgctggcgtt gagcgcgatg ttggccgtga caattcatct
     6361 ctagccaatt catctccagc attgacagcg aagccggctt gcctgatttg agcagcggcg
     6421 ttgccgggtt tttcggcgtc gcaacgggat tggcaatgac agccgtgacc aaggcccgag
     6481 ccgcatgcct gatcggatgg ccggccgcgc attcgcgctc gccgctgatc catcactatt
     6541 ggctgcgcgc gctcggcatc gagggcggct acaatatcga ggcggtgccg cccgagggtt
     6601 ttgccgagtt cgtgctgcac ttgtcgaccc atggctttgt cggcgccaat gtcaccattc
     6661 cgcacaagga gcgggtgctg gcgctgacga agccggacga acgcgcccgg gcggtcggcg
     6721 cagccaatac gctgtggtac gaaggcgccg aactgtgctc gaccaatacc gacatcgaag
     6781 gttttatcgg caatctcgac gcctgcgcgc aaggctggga tgctgctggc gatgcgctgg
     6841 tgctgggcgc cggcggctcg tcgcgcgcgg tggtgttcgg actgatcgag cgcggcatca
     6901 gccgcgtaca tctggcgaac cgcaccctcg agcgcgcgca ggcgctggcg catcaattcg
     6961 gccccagtgt tcaccccgtc gcatgggatg ccatcggcgg cctgctgccg cgcgcgggcg
     7021 tcttggtcaa cacgacgtcg cttggcatgc acggccagcc cgcgctggag ctcgacgccg
     7081 gcctgctgcc gtcacatgcc gttgttgcag atctcgtata tgtgccgctg gaaacaccgt
     7141 tgctcgtcgc ggcgcgggcg cgcggactga aaaccgccga tgggctcggc atgctgctgc
     7201 accaggcggt gcgcgggttc gaactctggt tcggccagcg ccccgaggtg acgtccgagc
     7261 ttcgcgcgct ggttgaggcc gatctcacaa aggtttgagc ccggaatatc gctcaagcgc
     7321 cggtgtccca tgttaggtgg tgccaccgga gaccagctca tgcccgttcg tcgctcaacc
     7381 ctcatccgtc cacttgtcgc agtcgccatg gtcgtggcgt cggctttgta cgcgatcgcc
     7441 cagcagccgc cgtcgccgtc gcgggttcgc ggcaccattg aagccattga cggcgatgtg
     7501 ctgtcggtca agtcgcgcag cggcgaggac gtcaagctgc gcatgaccgg cgacatgcgg
     7561 gtggtgggga ttacaaggat atcgctgtcc gacatcaagg tcggctcctt catcggcacc
     7621 accacggtgc ccggacccga cggcagccag gacgcggtcg aggtgcatgt gtttccggaa
     7681 gacatgcgcg gcaccggaga aggctcgcgg ccctacgacc tgcgccccaa cagcaccatg
     7741 accaatgcga cggtggccca gaccgtcgcg ggcaatgacg gtcacacgtt gatgatcaaa
     7801 tacaaggacg gcgagaagaa agtcgtggtt tcgccggaca ccccggtcgt gacctatgtg
     7861 ccggccgaca aatccgacct gaaggccggc gccaaggtga tcgccttcat gaaacagctg
     7921 ccggatggca cgttcgaaac caaccgcgtc agtgttggcc gcgatgggct gacgccgccg
     7981 atgtgaaggc cgccggcagg cggaatagcc ttggcgctga ggtgttttcc gcattgccag
     8041 ccaaaaccca ggaaggggaa aaccatgcca ggaatttctt cgttgatgcc gcgtgcactt
     8101 gcggcagcag tgacattgtc ggtacttgca tcgtcggtgt tcgcatcggt cgcgtgggcg
     8161 cagcaacctc cgccggtccg catccgcggc accatcgaaa gcgtcgacgg cgccatgctg
     8221 atgatcaagt cgcgcgaagg caccgacatg aaggtgcgcg tgaccgacaa tgtggcggtg
     8281 ttcggcgtcg ccaagaccga gatgtcggaa atcaagccgg gctcctacat cggggtatcg
     8341 gcgatgccgg agcccgacgg cacccagaag gcgcttgcgg ttcatatctt cccggaaacc
     8401 cagcgcggcg cggccgaagg tttcaggccc tgggatctcc gccccaactc aaccatgacc
     8461 aacgccaccg tcgccgagac ggtcaagggc accgacggcc agaacatcct ggtgaaatac
     8521 aaggacggcg aaaagaaagt cgtggtgccg ccggacacgc cgatcgtgac tttcgtcgcc
     8581 ggcgacaagt ccgaactgaa gccgggcgcc aagatcatca tcttcggcgc ggtgaagaaa
     8641 gacgacggca cgctggaagc caaccgcgtc aatgtcggcc gcgacggcat caccccgccg
     8701 atgtgaactt acttcccctc tccccttgga cgcgatgcga agcatcgcgg acgggtcagg
     8761 ggtttctttc tatcgatagg ccgtgacccc tcatccggcg cggacttcgc atagccgatg
     8821 caaagcatcg gcgtccaatt agcgaacggc ggccgaaggc cgcctgcggc gaccttcacg
     8881 cacaagcgga gaagggaagt gaagaggccg cgacgacgat ggattttcga tcgtttgcct
     8941 tcaaaccgcc agcacgtgat cgtcgatctc ggcgatggtg ttgacgccgc acagccccat
     9001 ggtcacgctc agttcgttgg cgatcagatc gatcgctttg gcgacgccgg ggccgccgaa
     9061 ggcgccgagg ccatgaatgt aggcgcggcc gatcatgcag gatttggcgc cgagcgcgag
     9121 cgcgcgcatc acgtcctggc ccgagcgaat gccgccgtcg aacatgactt ccatctgcga
     9181 gccaacgaca tcgacgatct ccggcagcac ttcgatcgac gacggcgcgc catcgagctg
     9241 ccggccgccg tgattggaga ccaccagcgc ctgggcgccg gtcttcgccg cttcctcggc
     9301 gtcctcgacg tcgagaatgc ccttgaggat cagcttgccc ggccagatgc tgcggatcca
     9361 ctcgacgtct ttccagttca gcgtggtatc gaattgcgtc gagatccagg tccccagcgc
     9421 ggtgatgtcg tcggtgccct tgaggtggcc catcagattg ccgaaggtgc gatgcttgcc
     9481 gcgcagcacg cccgataccc atccaggctt gctggcgaaa tccaggaatt tcgacacgct
     9541 ccattccggc ggcaccgtca tgccattctt gatgtcggca tgacgctgtc cgatcacctg
     9601 caggtcgacg gtcaagacca gcgcgctgca tttcgccgcg atcgcgcgct cgatcagcgc
     9661 cttgatgaag ccgcgatcct tcatgacgta caattgaaac cagaacggct tgtcgacatt
     9721 ttcagcgaca tcctcgatcg agcagatcga catcgtgctg agcgtgaagg gaatgccggc
     9781 ggcctgcgcg gcgcggcagg cgtggatctc gccatcgcca aactgcatgc cgcacaaccc
     9841 gatcggcgcc aggatcagcg gcagcgctgc gggctcgcca aggatggtgg ttgacagatc
     9901 gcgtttcgat acgtccacca ggatacgctg gcgaaacttg atttgctgca ggtcgtcgcg
     9961 attggcgcgc agcgtttcct cggcatagga gccgcgatcg gcgtagtcga aaaacgcctt
    10021 cggcactttg cgcatatgga cctggcgcag atcctcgatg caggtgatgt gtttcatgga
    10081 cgttcccgac ccttcttgta attcttcgct ggcgcagtca tctagcatgg ttgggccagc
    10141 cgcaaaatcg tgtcgctgac tcgactggta ataggagggc atccatgacc ggaccacgct
    10201 ccgaccccaa ggacgtcaaa ggtgccgaga agcgccggct cgatcaggca ctcgaggagg
    10261 gtctggagga gacctttccg gcctcggacc cggtcaatgt cacccagccg ccgccctcga
    10321 aaggcgacca tcacgtcaag cgcaaggact gatgctgcat tgcgccaggg ttcggatctg
    10381 gtaataatgc gcgcgttaac aacgtgttag atgctcgcga ccggattggc cggcggtaat
    10441 gattgatcat attttttgaa gccattttag cgccgaacgg attataaggc gtacacgcta
    10501 tgataggccg gcgttcgagg cttctagcgg gctggtccag agaatccttg aaagctgctg
    10561 gggggaatat gagccgcaaa tatttcggta cggatgggat tcggggccgg gccaacggcc
    10621 tgatcacgcc ggaactggcg ctgaaggtcg ggcaggcggc tggattggtg ttccagcgcg
    10681 gcgagcatcg ccatcgcgtc gtgatcggca aggacacgcg gctttccggc tacatgatcg
    10741 aatacgcgat ggtggccggc tttacttcgg tcggcatgga tgtgctgctg ctcggcccga
    10801 tgccgacgcc ggcggtggcg atgctgacca agtcgatgcg cgccgatctc ggcgtgatga
    10861 tttcggcgtc gcacaatctg ttcgaggata acggcatcaa gctgttcggc ccgcaaggtt
    10921 tcaagctgtc cgacgatgtc gaaaggcaga tcgagcagtt gatggacgaa tcgctcgaca
    10981 agcgcctggc gcagagcgcc agtctcgggc gcgcccgccg catcgacggc gtccacgatc
    11041 gctacatcga attcgccaaa cgaacgttgc cgcgcgatct cagcctcgat ggcatgcgcg
    11101 tggtggtcga ttgcgcgcac ggcgcggcct atagggtggt gccggaagcg
//
LOCUS       DATNOI010000612        15334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_410630,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000612 DATNOI010000000
VERSION     DATNOI010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15334
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410630"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="VN968_24840"
     CDS             <1..1444
                     /locus_tag="VN968_24840"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HXL32493.1"
                     /translation="AAQERGMAGKAIVTLSRSSVEPFLKSSTRRDLREKVFKAFTARG
                     DNKNDNDNNAAIVEILSLREESAKLLGFSTFAAYRLEDSMAKTPEAVRGLLERVWKPA
                     RARALADRDALQALVAEEGGNFALAPWDWRYYAEKLRQRRANFDDAAIKPYLVLDHMI
                     DAAFDCATRLFGVTFSERKDIPVWHPDVRVWEVKDAAGRHKALFYGDYFARASKRSGA
                     WMTSLRDQQKLDGAVAPLIINVCNFSKGADGQPSLLSPDDARTLFHEFGHGLHGMLSD
                     VFYPSLSGTSVFTDFVELPSQLYEHWQEQPQVLRQFARHYQTGEPLPDDLLQRFIAAR
                     KFNQGFATVEFVSSALIDLEFHTQPASASRDVRAFERQELEKIGMPAEISLRHRPQQF
                     GHIFSGDHYASGYYSYMWSEVMDADAFGAFEEAGDIFDPATAKRLHDDIYSSGGSRDP
                     EDAYVAFRGREPEPDALLRRRGLLETPEAA"
     gene            1446..2090
                     /locus_tag="VN968_24845"
     CDS             1446..2090
                     /locus_tag="VN968_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HXL32494.1"
                     /translation="MNVARRLLGLLWLAGALVLAAGTAQAHPHVWITATSELIYAPDG
                     AITGVRHAWTFDDMFSTYALQGIETKTKGVYSREELSSLAQTNVESLKEFKFFTFAKA
                     DGKKEKFEEPVDYFLEYKDAALTLHFTLPLKTPLKSKELALEVFDPEFFIDFKFADKD
                     PVRLVGAPAACQMKFQRPNDGSANAQKLDEQTFLSGQNSNYGVMFANKITVACP"
     gene            2090..3160
                     /locus_tag="VN968_24850"
     CDS             2090..3160
                     /locus_tag="VN968_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HXL32495.1"
                     /translation="MQKSPSSFARGLAICAAAAAAILIFDGAMQALLAQTPFGGPRPS
                     AEPQVGGIIGWLLARQSEFYREMSSAIRAAKSDGSAVWTVLAVSFAYGIFHAAGPGHG
                     KAVISSYLIANQETARRGIVLSFASALMQSLVAVVIVAICAWLLNATAKTMCGAERAI
                     EILSYALIAAFGARLVWTKGGGFIRALQAPRPALAMAAAGLGDGRGHDHHHHHHDHDH
                     QGPDQHVHDEHCGHSHGPEPSELAGPGGWWRGFGAIFAVGIRPCSGAILVLVFTLAQG
                     AFWTGIAATFVMGLGTAITVAAIAVIAVSAKDLARRLSAGTEGGGTLVMRGVEFGAAG
                     LVLLFGVGLLFGYLAAERVTCL"
     gene            3262..3555
                     /locus_tag="VN968_24855"
     CDS             3262..3555
                     /locus_tag="VN968_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXL32496.1"
                     /translation="MERDEAMRTLQGHEADLKRLGVEHLYMFGSTARGEANNDSDVDL
                     FFDYQKGKLGVYELMDVKEYAASILGRKTDIMTRDSLHKTLREAIEATAVRVF"
     gene            3582..3914
                     /locus_tag="VN968_24860"
     CDS             3582..3914
                     /locus_tag="VN968_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HXL32497.1"
                     /translation="MTDIIEAIERVNGVLADVSLEAFEKDWQRQWLVERGVEIISEAN
                     RHLPDDLKARNPQIPWQKVAGIGNVLRHNYESIAAPVLWKLVQADLPTLEKACRSELT
                     AEESREHR"
     gene            4015..5499
                     /locus_tag="VN968_24865"
     CDS             4015..5499
                     /locus_tag="VN968_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXL32498.1"
                     /translation="MADIWARKSPAELLSEIAQGGGADGRARPLRRALSALNLVTLGI
                     GGIIGAGIFVLTGHAAAANAGPAVALSFMLGAVACAFAGLCYAELSSAVPISGSAYTY
                     AYATLGELIAWIIGWDLILEYAVGAATVAIGWSGYVVSLAKDFGLVLPARFVSPPLAF
                     DAATHAWTLTGAVLDLPAATVIILITAILVAGVRETARFNGFLVAVKLVVIVLFVAST
                     VSAFSTANWIAPANPQGAFIPPNAGPGVYGWSGLVRGAAVVFFAYIGFDAVSTTAQEA
                     KNPKRDVPIGILGSLVICTVLYAVVGFVLTGIVRFDKLNVADPIAVGIDAAGVGWLSP
                     FIKLGIIFGLTSVILVSLLGQPRIFRAMAHDGLLPPAFAKIHPRFRTPYVSTISTGVV
                     VAILAALLPIDLVGELVSIGTLFAFAVVSLGVLALRIAQPGLDRPFRAPAIWIVAPAG
                     AATSIFLMFGLPVDTWVRLAVWLAIGLVIYFAYGIRHSRLGSTK"
     gene            5630..6304
                     /gene="tenA"
                     /locus_tag="VN968_24870"
     CDS             5630..6304
                     /gene="tenA"
                     /locus_tag="VN968_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HXL32499.1"
                     /translation="MSFFERLKTAASAEWRAYTEHPFTNGLADGSLPEAAFRHYLVQD
                     YLFLIEFARAYALCVYKSPKLADMREAAAGLSAILDIEMNLHVKLCAGWGLSPGDLEK
                     APPAMEMLAYTRYVLDAGMRGDLLALKVALAPCVIGYAEIATRLASRPDAYAATNPYR
                     VWIAEYAGAPYQEVAAKARAHLEALADLYATPAREAELIVIFKEATRLEADFWEMGWR
                     ADRPVE"
     gene            6331..6612
                     /locus_tag="VN968_24875"
                     /pseudo
     CDS             6331..6612
                     /locus_tag="VN968_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(7222..8145)
                     /locus_tag="VN968_24880"
     CDS             complement(7222..8145)
                     /locus_tag="VN968_24880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32500.1"
                     /translation="MQRSKLPGQPNTAIEHRGNRRHRWRFPAASSHSFALDMLLSKGK
                     IVQASGATTLPASWCIFSGEGAPMCKRALAILTLVICSACALAQNESGAALQGIDRND
                     LCVTNGNVSVLSGGWLSIDTPSSRAVVRADAKDTADQVAEIHFRYLGPTQTSKPLASG
                     ELRRQIGLKLQAEDTCNLIYAMWHIEPDTMVAVSVKRNTGMHTHEQCHAGGYVNIKAQ
                     RRIDLPPIQPGQTHTLRAELHGNDLTVTADGNVAWYGSLGDEPLSLQGPTGFRTDNAR
                     FEFQYYADPPADAQTRRGAPGPEHCVMSEGD"
     gene            8295..10385
                     /locus_tag="VN968_24885"
     CDS             8295..10385
                     /locus_tag="VN968_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HXL32501.1"
                     /translation="MSSLAVKGNGIQSKLLWAAVIALGALSFGIVALNRGESVNAAWL
                     VIAAVCIYFIAYRFYALFIAEKVLGVDPGRQTPAYQHNDGLDYVPTNRYVLFGHHFAA
                     IAGAGPLVGPVLAAQMGYLPGTLWILAGVVFAGAVQDLTVLFLSTRRDGRSLGDMVRS
                     EMGPVAGGIALVGVLLIMVILLAVLALVVVKALVGSPWGTFTVFSTIPIAMLMGVYAR
                     FIRIGRIAEMSAIGVVLLLASLIYGRTVAENPAIATWFDLRGETLALIIIGYGFVASV
                     LPVWLLLAPRDYLSTFLKVGTMALLAVGIILVQPELKMPAVTKFIDGTGPVWSGSLFP
                     FLFITIACGSVSGFHALIASGTTPKMVENESQLRFIGYGAMLTESFVAIMAMIGASVI
                     EPGIYFAINSPGALIGTTAAQAAPVISGWGFTVTPDMISQIAQDVGEKTLLSRTGGAP
                     TLAVGMAHILSGMFGGRVWMGLWYHFAILFEALFILTTVDAGTRVARFMIQDLIGSVV
                     PSFRKTESWGNNLVGSALAVGAWGYFLYQGVIDPMGGINTLWPLFGISNQMLAGIALI
                     LCTVVLFKMKRQRYAWVTIVPTSWLLVCTLTAGWQKIFSAEPAIGFVAHAQVFGDAAA
                     AGKILAPAKTLEEMHRVIVNDYVDATLSALFVAVVLAMIAYGVVAIRRALGDPKITAI
                     EVGLSATIPGGNLA"
     gene            10390..10578
                     /locus_tag="VN968_24890"
     CDS             10390..10578
                     /locus_tag="VN968_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HXL32502.1"
                     /translation="MTLKEWSVRTARLMVGIPDYQTYVEHRRQKHPGEPIMTYQEFFV
                     ERQNARFAVGKGRFRGCC"
     gene            10921..11463
                     /locus_tag="VN968_24895"
     CDS             10921..11463
                     /locus_tag="VN968_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXL32503.1"
                     /translation="MSGIDSKQPNNLIATAYQGDVKTAIAQLEEGADVNARDCDGDTA
                     LILAAERGHTELVKVLLKNGADVNARNLNRETALMRAAANDRVVAVKILLAHQADGNA
                     GDIINCTPLMRAAYRGHVDVVKELLAYSVDAKARNAFGNTAATLAARNGHSEVESLLR
                     EANDIGLVDSVQPKLRAARR"
     gene            complement(12494..12892)
                     /locus_tag="VN968_24900"
     CDS             complement(12494..12892)
                     /locus_tag="VN968_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HXL32504.1"
                     /translation="MTVAEWCVFGAVMLYLLTIAPFKVTGFPRFDNSKPRDPAFFQDR
                     LRARALGAHINGIETFPFFAAAVLLAEFRTSPQNLVNELAVLFLIVRVAYVLTYLGDR
                     PTLRTILWNIGFAINLGIFFMPAIRGFLPV"
     gene            13068..14105
                     /gene="hemH"
                     /locus_tag="VN968_24905"
     CDS             13068..14105
                     /gene="hemH"
                     /locus_tag="VN968_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HXL32505.1"
                     /translation="MTAVIPIERSTAVAEVKPGRIGVLLVNLGTPDSADARGVRVYLK
                     EFLSDPRVIEDQGLLWKLALNGVILRVRPRRKARDYLKIWNTENNESPLKTITRAQSE
                     KLAAAIADHDHVVVDWAMRYGNPSIRSRIDALTGEGCDRLLVVPLYPQYSAATSATVC
                     DEVFRVLGDMRAQPTLRVTPPYYDDPDYIEALAVSINTHLKTLPFQPELIVASFHGMP
                     QKYVDKGDPYYAQCVATTDALRKRMGLDASKLILTFQSRFGFDAWLQPYTDQTTERLA
                     KEGVRRIAVVMPGFSADCLETLEEIAQENAEIFKHHGGEQFAAIPCLNDSDPGMDVIR
                     QLVLRELQGWI"
     gene            14289..15287
                     /locus_tag="VN968_24910"
     CDS             14289..15287
                     /locus_tag="VN968_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXL32506.1"
                     /translation="MTGFDIFAIAVALLVIFTLLAGVKTVPQGYNWTIERFGRFTRTL
                     SPGLNIIVPYFDRVGRKMNVMEQVISIPEQEVITKDNATVTVDGVAFYQVFDAAKASY
                     EVSDLDRAIIVLTMTNIRSVMGAMDLDQVLSHRDEINERLLRVVDAAVSPWGLKVNRI
                     EIKDIVPPADLVEAMGRQMKAERVKRADILQAEGQRQSEILRAEGAKQGQILQAEGRR
                     EAAFRDAEARERSAEAEAKATQMVSEAIAKGDVAALNYFIANNYIKAFGQFAGSPNQK
                     VIMLPIEAVSLLGSLAGIGEIAKATFGESAASAEAAARPPSVPQAGPNPPPATPQP"
BASE COUNT         2791 a         4695 c         4823 g         3025 t
ORIGIN      
        1 cgctgcccaa gagcgcggca tggccggcaa ggcgatcgtg acgctgtcgc gctcgtcggt
       61 cgagccgttc ctcaaaagct ccacccggcg cgatctgcgc gaaaaggtgt tcaaggcttt
      121 caccgcgcgc ggcgacaaca aaaacgacaa cgacaacaat gccgccattg tcgagatcct
      181 gagcttgcgc gaggagagcg cgaagctgtt gggcttttcc acctttgccg cctatcggct
      241 ggaggattcg atggccaaga cgcccgaggc ggtgcgcggc ctcctggagc gggtctggaa
      301 accggcgcgg gcccgcgcac tggccgatcg cgatgccctg caggcgctgg tcgcggagga
      361 gggcggcaat ttcgcgctgg cgccgtggga ctggcgctac tacgccgaaa agctgcggca
      421 gcgccgcgcc aatttcgacg acgccgcgat caagccgtat ctcgtgctcg accacatgat
      481 cgacgccgcc ttcgactgcg ccacccgcct gttcggcgtc accttcagcg agcgcaagga
      541 tattccggtc tggcatccgg acgtccgggt ctgggaggtg aaggatgcgg ccggccgcca
      601 caaggcgctg ttctatggcg actacttcgc ccgcgcctcg aagcgctccg gcgcctggat
      661 gacctcgctg cgcgaccagc agaagctcga tggcgccgtc gccccgctga tcatcaacgt
      721 ctgcaatttc tccaagggcg ccgacggcca gccgtcgcta ttgtcgccgg acgacgcgcg
      781 gacgctgttt cacgagttcg gccacggcct gcacgggatg ctgtcggacg tgttctatcc
      841 ctcgctgtcc ggcaccagcg tatttaccga tttcgtcgag ctgccgtcgc agctttacga
      901 gcactggcag gagcagccgc aggtgctgcg gcaattcgcg cggcactacc agaccggcga
      961 accgctgccg gacgatctgt tgcagcgctt catcgccgcg cgcaaattca accagggctt
     1021 tgccaccgtg gaattcgtct cctcggcgct gatcgacctg gaattccaca cccagccggc
     1081 ctccgccagc cgggacgtgc gggcgttcga gcgccaggaa ctggaaaaga tcggcatgcc
     1141 cgcggaaatc tcgctgcggc accggccgca gcaattcggc catatctttt ccggcgatca
     1201 ttatgcgtcc ggctattaca gctacatgtg gtccgaggtg atggatgccg atgccttcgg
     1261 cgcgttcgag gaggccggcg acattttcga ccccgcgacg gcaaagcgcc tgcacgacga
     1321 catctattcg tcgggcggct cgcgcgatcc cgaagacgcc tatgtcgcgt tccgcggccg
     1381 cgagccggag cccgacgcgc tgctgcgccg gcgcggcctc ctggaaacgc cagaggcggc
     1441 ctgaggtgaa cgttgcgcgc cgcttacttg gtctgttgtg gctcgccggc gcgcttgtgc
     1501 tcgccgccgg cacggcgcag gcccatccgc atgtctggat cacggcaacc agcgagctga
     1561 tctacgcgcc cgacggcgcg atcaccggcg tccgtcacgc ctggacgttt gatgacatgt
     1621 tctcgaccta cgcgctgcag ggcatcgaga ccaagaccaa gggtgtctat agccgcgagg
     1681 aacttagctc cctggcccag accaatgtcg aatcgctgaa ggaattcaaa ttcttcacct
     1741 tcgccaaggc cgacggcaag aaggagaaat tcgaggagcc ggtcgactat ttcctcgaat
     1801 acaaggatgc cgcgctgacg ctgcatttca cgcttccgct caagacgccg ctgaaatcga
     1861 aggagctcgc gctggaggta ttcgatccgg agtttttcat cgacttcaaa ttcgccgaca
     1921 aggacccggt cagactggtc ggagctcccg ccgcctgcca gatgaagttc cagcgcccga
     1981 atgacggctc ggccaatgcc caaaaactcg acgaacagac ttttctgagc ggccagaact
     2041 cgaattacgg cgtgatgttc gccaacaaga tcacggtggc gtgtccgtga tgcagaaatc
     2101 cccctcaagt ttcgcgcgcg gcctggcgat ctgcgccgcg gcggcggctg ccattctgat
     2161 attcgacggc gccatgcaag cgctgttggc gcagacgccg ttcggcggtc cgcgcccgag
     2221 cgccgaaccg caggtcggcg gtatcatcgg atggctgctt gccagacaat cggaatttta
     2281 tcgcgagatg tcctcggcca ttcgcgccgc gaagtccgac gggtcagcgg tgtggacggt
     2341 gctcgcggtt tcgttcgcct atggcatctt tcacgccgcc ggtcccggcc acggcaaggc
     2401 ggtgatctcg tcctatctga tcgccaacca ggaaaccgcg cggcgcggca tcgtgctgtc
     2461 gttcgcatcg gcgctgatgc agtcgctggt cgcggtcgtg attgtcgcga tttgcgcctg
     2521 gctgctcaac gccacggcca aaaccatgtg cggtgccgaa agggcgatcg agattttaag
     2581 ctacgcattg atcgcggcgt tcggcgcccg gctggtctgg accaagggtg gcggattcat
     2641 ccgcgcgctg caggcgccgc ggccggcgct cgcgatggcg gcggccggcc ttggtgatgg
     2701 ccgcggccat gatcaccatc atcatcatca tgaccatgac catcaaggcc ctgaccagca
     2761 tgtccatgac gagcattgcg gccactccca cggtcctgag ccaagcgaac tcgccggacc
     2821 cggcggctgg tggcgcgggt ttggcgcgat ttttgccgtc ggcatcagac cctgttcggg
     2881 cgcgatcctg gttctggttt tcacgctggc acagggggcg ttctggaccg gcatcgccgc
     2941 gaccttcgtg atggggctcg gcaccgccat cacggttgcg gccatcgccg tgatcgcggt
     3001 gtcggcgaaa gacctggcgc ggcgtttgag cgccgggacg gaaggcggcg gcacgctggt
     3061 gatgcgtggc gtggaattcg gcgcggccgg tctcgtgctg ctgttcgggg tcggcctgct
     3121 gttcggctat ctcgccgccg agcgcgtgac ctgtttgtga gaagttcaat tgatggccgg
     3181 ttcgcctttc tccgcaccgt ataggtcttg gcgaaaccga aagacgtgga aacagccgct
     3241 cggatttgct ataatattgt tatggaacgc gatgaagcca tgaggacatt gcaggggcac
     3301 gaagccgatt tgaagcggct tggcgtcgaa catctttaca tgttcggctc gacggcgcgt
     3361 ggcgaggcga acaacgattc cgacgtggac ctgtttttcg actatcaaaa gggcaagctc
     3421 ggcgtgtacg aactcatgga cgtgaaggaa tacgccgcca gcatcctcgg ccgtaagact
     3481 gacatcatga cccgcgacag tctgcacaag accctgcggg aagcgataga agcaacagcc
     3541 gtccgcgtgt tttagtgatc gcacgttcgc tcatccctcg tttgacggac atcatcgagg
     3601 cgatcgaacg cgtcaatggc gtattggcgg atgtgtcgct cgaagcgttc gaaaaggatt
     3661 ggcagcggca atggctcgtc gagcgcggtg ttgagattat ttccgaagcc aatcgtcatc
     3721 ttccggatga cctgaaagcg cgcaatccgc aaatcccctg gcagaaagtt gcggggatcg
     3781 gcaacgtact gcgccacaac tacgagagca tcgccgcacc cgtcctgtgg aagctggtgc
     3841 aagccgattt gccgacattg gaaaaagcct gccgctctga actcaccgcg gaagaaagtc
     3901 gggagcaccg ctgatatttg gacaaggtgg ggctcggccg cgcgtcgcag ccggatgcca
     3961 gccgaacatt ccagaactct tcgaaccaat tccagcattg gaaactgatt cccgatggca
     4021 gacatttggg cccgcaaatc ccccgccgag ttgttgagcg aaatcgcgca aggtggcggc
     4081 gcggacggcc gcgcgcggcc gctgaggcga gcgctgtctg cacttaacct cgtgacgctc
     4141 gggatcggcg gcatcatcgg cgccggcatt ttcgtgctga cggggcatgc cgccgcggcc
     4201 aatgccgggc cggcggtagc gctttcgttc atgctcggcg cggtcgcctg tgcgttcgca
     4261 ggattgtgct acgccgaact gtcatcggcc gtcccgatca gcggcagtgc ctacacctac
     4321 gcctatgcca cgctgggcga gttgatcgcc tggatcatcg ggtgggacct gatcctcgaa
     4381 tacgccgtgg gggccgccac ggtcgccatc ggctggtccg gatatgttgt cagtcttgca
     4441 aaagactttg gactggtgct tccagcccga tttgtttcgc cgccattggc cttcgacgcg
     4501 gcaacgcatg cctggacgtt gacgggagcg gtcctcgatc tgccagcagc taccgtgatt
     4561 atcctgatca cagcgatcct ggtggcgggc gttcgcgaga cggcgcgctt caacggcttt
     4621 ctggtggcgg tgaagcttgt cgtgatcgtg ctgttcgtcg caagtaccgt gtctgctttc
     4681 agcaccgcga actggatcgc acctgctaat ccgcaaggtg cgttcattcc gcccaatgca
     4741 ggtcccgggg tttatggctg gtcgggcctg gtgcgcggcg cggccgtggt gttttttgcc
     4801 tatattggat tcgacgcggt gtcgaccacg gcccaggagg cgaagaatcc gaagcgggac
     4861 gtgccgatcg gcattctggg ctcgctggtc atctgcacgg tgctgtatgc cgtggtcggt
     4921 ttcgtgctca cgggcattgt tcgcttcgac aaattgaacg ttgccgatcc gattgcggtc
     4981 ggcatcgatg cggccggcgt gggctggctt tcgccgttca tcaagctcgg aattatcttc
     5041 ggcctgacgt cggtcattct ggtctcgttg ctcggccagc cgagaatttt tcgcgcgatg
     5101 gcgcatgacg gcctgctgcc gccggccttt gccaaaatcc atccgcggtt tcgcacaccc
     5161 tatgtctcga cgatctcgac cggcgtcgtc gtggccatcc tcgccgcgct gttgccgatc
     5221 gatctggtgg gcgaactggt cagcatcgga acgctgtttg cattcgccgt ggtgtcgctc
     5281 ggcgttctgg cgctgcggat cgcgcagccg ggactcgatc gtcccttccg ggcgccggcg
     5341 atctggatcg ttgcgccggc cggcgccgcg acgtcgatct tcttgatgtt cggcctgccg
     5401 gtcgatacct gggtgaggtt ggcggtgtgg cttgcgatcg gcctcgtgat ctattttgcc
     5461 tatgggatcc gacacagccg gcttggctcg acgaaatagg tagccgctgc cttgacgggt
     5521 gtccgctttt gcgggaagag cgaatagtgt tcttggcctg aaaggtctgc cgatcacccg
     5581 gcagcgagcc aaagccacgc gattgagaag caaaccctgc gaaaacatca tgagtttctt
     5641 cgagcgtctg aagaccgcgg catccgccga gtggcgggcc tacaccgagc accccttcac
     5701 caacgggctg gccgacggtt cgctccctga ggcggcgttt cgtcactacc tcgttcagga
     5761 ctacctgttc ctcatcgagt tcgcccgcgc ctacgcgctc tgtgtctaca agtcgcccaa
     5821 gctcgccgac atgcgcgaag cggcggccgg cctctcggcc attctcgaca tcgagatgaa
     5881 cctgcatgtg aaactctgcg ccggttgggg cctgtcgccc ggcgatcttg aaaaggcccc
     5941 tccggcgatg gagatgctgg cctatacccg ctacgtgctc gacgcaggga tgcgcggtga
     6001 cctgctggcg ctgaaggtgg cgcttgcgcc gtgcgtgatc ggctacgcgg agatcgcgac
     6061 gcgcctcgcc tcgcgccccg atgcgtacgc tgcaaccaac ccgtatcgcg tttggatcgc
     6121 cgagtacgcc ggtgcaccct accaggaggt cgcggcgaaa gcgcgggcgc acctcgaagc
     6181 tctcgccgat ctctacgcca cgccggcccg cgaggcagag ctgatcgtga tcttcaagga
     6241 ggccacccgg ctcgaagccg acttctggga aatgggctgg cgtgcggatc ggcctgttga
     6301 atagtcgctg accgcgccga gtaagtgctc gcacgtctca tgcatgtcag cccggaccag
     6361 cggggcgtca tttccggcat gctcagcctg tcgcgcaatc tcgggctcat caccggcgca
     6421 tccgtcatgg gtgcggtgtt cgcagctgct tcggcgacga tcgacatcac gacggcgcgc
     6481 cccgaggccg tggcaaccgg tatgagcatc acgttcgcgg tcgcggctgt tctgatcgtc
     6541 ggcgcgctcg ccatcgcggt tgcaagccgt gctctcgcaa cacgccattc gcgccctgga
     6601 gacgttcgat agatgtactg ctgttgaggg aagcgcggac gaacgtgatg tctgacttga
     6661 gcccgcaatg ggttccgaag cggacatcgg cgagagaaac ataggggcct gttcaaggcg
     6721 gtgaccctaa atcgcgggcg cctgatgttc cgcgcggatg aaacttcgtc aagttcccat
     6781 ttctccgtct gtacgctttt gtgccttgga gccggaacaa ttccccacct cccgcgtgat
     6841 ctatctgggt tggaccatag ccaaggagaa gtgcagtgcc cggcagagag agcaagctcc
     6901 agcccgagag cgtcatggcc gatgtttgtc tcggcgtaac cgttgcaggc ttatacgcgt
     6961 tagtcggaat ggggctcacg atcgcgttta tgtgaaggcc tgtttgatgt gagcctagag
     7021 ccccagcgcg gctccgtggc acgctcgaac atggcagggc gtcgacctgc catgacgggc
     7081 gccgccgcgc tcggcgatgt tcccggccct gttgtcggca tttttattgt gcctggccct
     7141 tccatcggat ggggcaggga ttcctcgtga tccagcaccg ccgagatcgc ctttccctcc
     7201 tggatgttac ggcggcgtaa atcagtctcc ttcggacatg acgcaatgct cgggaccagg
     7261 cgcgccacgt cgcgtctgtg catccgcggg agggtcggca taatattgaa attcgaaccg
     7321 cgcattatcg gtgcgaaaac ccgttggtcc ttgcagcgac agcggttcgt ctccgaggct
     7381 gccataccac gccacgttgc cgtcggccgt cacggttaga tcatttccgt gaagttccgc
     7441 gcgtagtgtg tgggtttgac ccggctgtat cggcggcaaa tcgatgcgcc tttgcgcctt
     7501 gatgttcacg tatccacccg cgtggcactg ttcatgggtg tgcatcccgg tgttgcgctt
     7561 gacagagacc gcgaccattg tgtctggctc gatgtgccac atggcataga tcaaattgca
     7621 ggtgtcctcg gcttgcagct tcagtccgat ttgacgtcgc aattcgcctg atgccagggg
     7681 tttgctggtt tgagtggggc caagatagcg gaagtgaatt tccgcgacct gatccgcggt
     7741 gtccttcgcg tcggcgcgaa cgaccgctct ggagcttggc gtatcgattg aaagccagcc
     7801 gccggacaac accgaaacgt tgccattggt cacgcaaaga tcgttacgat cgattccctg
     7861 cagggcagct ccggactcgt tctgcgcaag agcgcaggcg gagcagatga cgagtgttag
     7921 aattgccaat gcgcgcttgc acatgggagc gccttctccg gaaaagatgc accacgatgc
     7981 cggcaaagtg gttgctcccg acgcttgaac gatcttcccc tttgacaaga gcatgtccag
     8041 cgcgaagctg tgggaacttg ctgccggaaa gcgccaacgg tgccgcctgt taccgcgatg
     8101 ctcaatcgct gtgttgggct ggccgggcag cttgctgcgc tgcactagtt gccgctccat
     8161 ttatgttgcg catggtttga tccgaataaa tgcgggctct ttcggcggga aaatgataaa
     8221 ttgtctcgtc cgaattaccc caacgtcaga aatggatcga gccgccacca aggaagccag
     8281 gattggaggg agccatgagt tcgctcgccg tcaagggaaa tgggattcag tcaaaactcc
     8341 tttgggccgc agtgatcgcg ctcggtgcgc tttctttcgg gatcgtcgcg ctcaaccgag
     8401 gcgagagcgt caacgcggca tggctcgtca tcgccgcggt gtgcatttac ttcatcgcct
     8461 accgctttta cgcgctgttc atcgccgaaa aggtactcgg cgtcgacccc gggcggcaga
     8521 cccccgccta ccaacacaat gatggcctcg attacgtgcc gaccaatcgc tacgtgctgt
     8581 tcggtcacca cttcgcggca attgccggcg ccggtcccct ggttggtccg gtgctcgccg
     8641 cccagatggg ctacctgcct ggcacgctat ggattctcgc cggcgtcgtc ttcgccggcg
     8701 cagtccagga tctgaccgtg ctctttctct ccacgcggcg tgacggccgc tcgctcggtg
     8761 acatggttcg ctctgaaatg ggtccggttg ccggcggcat tgcactcgtc ggcgtgctgc
     8821 tgatcatggt catcctgctc gccgtgctgg cgctggttgt ggtgaaggcg ctggtcggca
     8881 gtccatgggg caccttcacg gtgttcagca ccattccaat tgcaatgctg atgggcgtct
     8941 atgcccgctt catccggatc ggccgcatcg cggagatgtc ggcgatcggc gtcgtgttgc
     9001 tgctggcgtc cctgatttac ggccggaccg tagcggaaaa cccggcgatc gcaacatggt
     9061 tcgatctgcg cggcgagacg ctggcgctga tcatcatcgg gtacggcttc gtcgcctcgg
     9121 tgctgccggt gtggctactg ctggcaccac gggactatct gtcgaccttc ctcaaggtcg
     9181 gcacaatggc gctgctcgcg gtcggcatca tcctggtcca gccagagttg aagatgccgg
     9241 cggtcaccaa gttcatcgac ggcaccggcc cggtgtggtc tggcagcctg ttcccgttcc
     9301 tgttcatcac catcgcctgc ggctcggtat ccggcttcca tgcgctgatc gcctcgggca
     9361 cgaccccgaa aatggtggag aatgagagcc agctccgctt catcggctat ggcgccatgc
     9421 taacggaatc cttcgtcgcc atcatggcga tgatcggcgc gagcgtgatc gagcccggaa
     9481 tctatttcgc catcaacagc ccgggcgcgc tgatcggcac cacggcggcg caggcggcgc
     9541 cggtgatctc cggctggggt ttcaccgtca cccccgacat gatcagccag atcgcccagg
     9601 acgtcggcga gaagacgctg ctgtcgcgca ccggcggcgc cccgacactc gcggtcggca
     9661 tggcgcacat cctgtccggc atgttcggcg gccgcgtctg gatggggctc tggtaccact
     9721 tcgccatcct tttcgaggcg ctgttcatcc tcaccactgt cgatgccggc acccgcgtcg
     9781 cccgcttcat gatccaggat ttgatcggca gcgtcgtgcc gtcgttccgg aagaccgagt
     9841 cgtggggaaa caacctggtc ggatcggcgc tggcggtcgg cgcctggggc tacttcctct
     9901 accaaggggt catcgatcct atgggcggca tcaacacgct ctggccgctg ttcggcattt
     9961 ccaaccagat gctggcgggg atcgcgctca tcctgtgcac ggtcgtgctg ttcaagatga
    10021 agcgccagcg ctacgcctgg gttacgatcg tgcctacctc atggctcctg gtatgcacgc
    10081 tgacggcggg gtggcagaag atcttcagcg ccgaaccggc gatcggcttc gttgcccacg
    10141 cgcaagtatt cggcgacgcc gccgcggctg gcaagatcct ggcgccggcg aagacgttgg
    10201 aagagatgca ccgcgtcatt gttaacgatt atgtcgacgc cacattgtcc gcgctattcg
    10261 tcgcggtggt gctggcgatg atcgcctacg gtgtcgtcgc catacgccgg gcgctgggcg
    10321 atccgaagat caccgcgatc gaggtcggcc tgtccgcgac catcccggga gggaaccttg
    10381 cctgacatca tgacgctgaa ggaatggtct gtacgcaccg cgcgtctgat ggtcggcatc
    10441 cccgactatc agacctacgt ggagcatcgc cggcagaagc atccgggcga gccgatcatg
    10501 acctaccaag aattcttcgt ggagcgccag aacgctcgct tcgcggttgg caagggccgc
    10561 ttccgcggct gctgttgagc gcaaagttcc tcgctcctcc agcggaggcc ggcaagcaac
    10621 gcactgccgc gtagtcaacc ttgcgggaag cgactcggac atcgtcagac cgacggggat
    10681 cacccaattc ggaagtcggg gctcaccaga cagtgtactg ccgcgatgca acctgcgact
    10741 ggtttgtcgc aaagcgaccc aagccggctt gagccaggcg ttgttgcaat attgtccgtg
    10801 tgcgctgcaa aattgatccg tagtactacc ctcagcgata ttgttccttc actttcatct
    10861 gggggctcgt atattcccgg cgtttcggac agcggggttt gacggtaagg ggggattgtc
    10921 atgagcggga ttgattcgaa gcagcccaat aatttgatcg cgactgccta tcagggcgac
    10981 gtcaagacgg cgatagccca gcttgaggag ggagccgacg tgaatgcgcg cgactgcgac
    11041 ggcgacacgg cacttatact tgccgccgaa cgaggccaca ccgagctggt gaaggtgctt
    11101 ctcaagaacg gagctgatgt caacgccagg aacctcaacc gcgagaccgc gctgatgcgg
    11161 gcggcggcga acgaccgagt ggtagcagtc aaaattctgc ttgcccatca ggctgacggc
    11221 aacgccgggg acatcatcaa ctgtacgcct ctgatgcggg ccgcgtatcg cggccacgtt
    11281 gatgttgtga aagagctgct tgcctatagc gtcgacgcga aagctcgcaa tgcatttggc
    11341 aacacagctg ccactctcgc cgcgcgcaat ggtcacagcg aggtcgaaag cttgctgcgg
    11401 gaagccaatg acatcggtct tgttgactcg gtccaaccca aactccgcgc ggcgcgccgc
    11461 tagaccctca ctggggagca tcgcctaagc gatgcgtctc gagcgaggag ggcccaatcg
    11521 tggcctcgtg attctctcgg cgaagccaac gggtcgcgcg aatgcgcacc cgacgtccga
    11581 cttgagtgcg caatgcgcat caaaacggac gtccgccaag gcctacgcat tgatgagttc
    11641 acgccccagg cgtggtttat ttgttgcaac ggcacagcct gctgatttgc ccgtcgggcg
    11701 gtttgttgac aggggtgtcc agtcccattt gcaaaaatat ttcacttcac ccgtcgggca
    11761 aatcatctct acaaattcgc gccatcccac cccaccagag gggcgtatca tgatcgtcac
    11821 ggacgcgggg agggatgcgg tggacgcggc agcgttttgc gcgcgacggg attgcagggc
    11881 aggtcgatga ggcctgtgag cgatcaccaa gcgagcggac gagagatgct gcagcgtacg
    11941 gcgaagtcgt gtggtcctga cgccccgacg ctggcgtcaa gttcgcggag gtttggtcgg
    12001 ccctaccggg cgcaggcaaa ccatatccgc ggatgacggt ggcaaaagag cccggtcacc
    12061 ggggagagcg cgaaggaaac cgttaaaacc attgcgtgcg ggaatgccgg gcgattccgg
    12121 tgtgctcgtt gttactcgtg tgcgttctac caatacaaag tgcacacgag gccgcgggtg
    12181 cagcgggcac ccggcattcc ccacgccctc caagggggcg gaagataaat gcacaactcg
    12241 ggcgcatgga gccgcgagat cgcgaaggcg tgtctgcgaa atccgttttt cgccatgacc
    12301 gggctgaagc gcgaagcgcg tcttcgcgca agatgtcccg gccatccacg tctttctggc
    12361 tgatacactc tcggatgccc gggacaagcc ggcatgacgg gtgtcgtcaa ttccgtggac
    12421 tgcatttaca gtcacgcgcg aaaaataagg ggatggtttg atctaccgtt tcgttccgac
    12481 gccatcatcc ccctcagacc ggcaaaaagc cccggatcgc cggcatgaag aaaatcccga
    12541 gattgatcgc gaagccgatg ttccaaagga tggtgcgcag cgtaggccgg tcgccgagat
    12601 aggtgaggac ataggcaacc cgcacgatca ggaacagaac cgcaagctca ttgacgagat
    12661 tctgcgggct ggtgcgaaat tccgccagca gcacagcggc agcaaagaac ggaaaggttt
    12721 cgatcccgtt gatgtgcgcg cccagcgccc gcgcccgaag gcgatcttgg aagaacgcgg
    12781 gatcgcgcgg tttggaattg tcgaagcgcg gaaaaccggt caccttgaac ggcgcaatcg
    12841 tcagcagata gagcattacc gctccaaaaa cacaccactc agcgaccgtc atggttcccc
    12901 ccgacgcgca cggcaatttt cccggggcat gatcttttcg gaaaaccggt atccaccccg
    12961 catcacgtgc ggggcaggcc ttttccggat catgccccaa tgcgtgacaa tatcgaaagc
    13021 gccattatct tgacaagcat ggtgcaacgc gcgaagcccc tgatattatg acagcggtta
    13081 ttcccatcga gagatcaacg gcggtggccg aggtgaagcc gggacggatc ggcgtgctcc
    13141 tggtcaatct cggcaccccc gacagcgcgg atgcgagggg cgtgcgcgtc tatctcaagg
    13201 aatttctgtc cgatccgcgg gtgatcgaag atcaggggtt gttgtggaag ctggcgctga
    13261 acggcgtcat cctgcgcgtc cgcccccgcc gcaaggcgcg cgattacctg aaaatctgga
    13321 acaccgagaa caacgaatct ccgctgaaaa ccattacgcg tgcgcagtcc gaaaaactcg
    13381 ccgctgccat tgccgaccat gaccacgtcg tggtcgactg ggcgatgcgc tacggcaatc
    13441 catcgatccg ctcgcggatc gatgcgctga cgggagaggg ctgcgaccgg ctgctggtgg
    13501 tgccgctcta tccgcaatat tcggccgcga catccgccac cgtgtgcgac gaagtgtttc
    13561 gcgtgctcgg cgatatgcgc gcgcagccga cgctgcgggt gacgccgccc tattacgacg
    13621 atccggatta tatcgaggcg ctggcggtct cgatcaatac gcatctcaaa accttgccgt
    13681 ttcagcccga attgatcgtg gcctcgttcc acggcatgcc gcagaaatat gtcgacaagg
    13741 gcgaccccta ttacgcgcag tgcgtcgcga ccacggacgc gttgcgcaag cgcatgggcc
    13801 tcgatgcctc aaaactgatc ctcacattcc agtcgcgctt cggcttcgat gcgtggttgc
    13861 agccctatac cgaccagacc acggagaggc tggcgaagga gggcgtccgc cgcatcgccg
    13921 tggtgatgcc cggcttttcc gccgattgtc tggaaacgct ggaagaaatc gcgcaggaaa
    13981 acgccgagat cttcaagcat catggcggcg agcaattcgc cgctattccc tgcctcaacg
    14041 acagcgaccc cggcatggac gtcatccgcc aactggtgct gcgcgagctt cagggctgga
    14101 tttgacggtc cagtgacgcc atacaaatcg ccggagttcg gatgctatgg atctggcggg
    14161 cggcaaacca gggcctacct atgttcaaga ctcggctata ttcaacaaca gccggctgaa
    14221 ccgtcgcggc cggcctgcag acaaaacaag acggcgctcg gcgtgtcgcg ctcggctttg
    14281 gaggggttat gaccggtttt gatattttcg ccatcgcggt tgcgctgctg gttattttta
    14341 cgctgttggc cggcgtcaag acggtgccgc aaggctataa ctggaccatc gagcggttcg
    14401 gcagattcac ccgcacgctg tcaccggggc tgaacatcat cgtcccctat ttcgaccgcg
    14461 tcggccgcaa gatgaacgtg atggagcagg tgatcagcat tcccgaacag gaggtgatca
    14521 ccaaggacaa cgccacggtg acggtggacg gcgtcgcgtt ctaccaggtg tttgatgccg
    14581 cgaaagcgag ctacgaggtg tccgatctcg accgggcgat catcgtgctg acgatgacca
    14641 atatccgctc ggtgatgggc gcgatggacc tcgaccaggt gctgtcgcat cgcgacgaga
    14701 tcaacgagcg attgctgcgc gtggtcgatg ccgcggtgtc gccgtgggga ttgaaagtca
    14761 accgcatcga gatcaaggac atcgtgccgc ccgccgacct cgtcgaggcg atgggacggc
    14821 agatgaaggc cgagcgcgtc aagcgcgccg acattctgca ggccgagggc cagcggcaat
    14881 cggaaatcct gcgcgccgaa ggcgccaagc agggccagat cctgcaggcc gagggccgcc
    14941 gcgaagccgc gttccgcgac gccgaagcgc gcgaacgttc cgcggaagcc gaggccaagg
    15001 cgacgcagat ggtctctgag gctatcgcca agggcgatgt cgccgcgctg aactatttta
    15061 ttgccaacaa ttacatcaag gcgttcgggc aatttgcggg ttcgccgaac cagaaggtga
    15121 tcatgctgcc gatcgaggcg gtgagcctgc tgggctcgct cgccggtatc ggcgagatcg
    15181 ccaaggccac cttcggcgaa agtgcggcgt ccgcggaggc cgccgcgcgc cctccgtcgg
    15241 tgccgcaagc aggcccgaac ccgccgccgg cgacgccgca accctgaggg tcatatcatg
    15301 accgagatgc tttacacgtt gggcacctgg aact
//
LOCUS       DATNOI010000613        13805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2214687,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000613 DATNOI010000000
VERSION     DATNOI010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13805
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2214687"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..1914)
                     /locus_tag="VN968_24915"
     CDS             complement(226..1914)
                     /locus_tag="VN968_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HXL32507.1"
                     /translation="MGSVVLLDLMGGVALLLWGLHMVHSGILRAFGPDLRLLLAKALN
                     NRFKAFAAGIGLTALLQSSTATALITSSFTAEGLVSLVPALAIMLGANVGTTLIVQIL
                     SFNIAAIAPVLFIIGLVAFRSGARSRIKDIGRVSIGLGLMLLALHILLDTLAPAENAP
                     GMRVFMSAITADPVLCIVIGAIVTWAVHSSVASVLLVMSLAYAQFISPDASLALVLGA
                     NLGSAINPVLEGARRDNPASYRLPVGNLINRLVGVLLVAPFLHPITETLRAWQPDLAK
                     LTAEFHIVFNVATAIIFIGLLDGMARLLKRLLPNRVQETDPSRPRYLDESALETPSLA
                     LADAARETLHMGDLVEVMLRKVMAAMVTNDRALVDQVSRMDNAVDSLDEAIKLYVTKL
                     TRGSLDEREGQRAMEIISFAINLEHMGDIIDKNLSELATKKIKRRFQFSPEGAEELSA
                     FHKRIMDSLRIAFGVFMSGDVNEARKLLAEKALLRNTELAATERHLDRLREGRPETIE
                     TTSLHLDVLRDLRRIHSHICSVAYPVLDAAGELAAYRSTESDVAALPAAHPLPR"
     gene            complement(1946..2161)
                     /locus_tag="VN968_24920"
     CDS             complement(1946..2161)
                     /locus_tag="VN968_24920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32508.1"
                     /translation="MPGLIDGDFALSESSAKERFLPLTARGSAAFRAARRAGGEPARA
                     QPSGGCRCISVLFQRNKRLIRRRLAIA"
     gene            2297..3685
                     /gene="mgtE"
                     /locus_tag="VN968_24925"
     CDS             2297..3685
                     /gene="mgtE"
                     /locus_tag="VN968_24925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017822053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HXL32509.1"
                     /translation="MTDLDLQDDPAAKDDNMVDPAFLAASMIEERAPDIVEALNDMPP
                     ELAVAVLLHLPPDRAIEVLDQPGLDREPELFTLMPREAAAKLLAGVSADRVADIFRQV
                     KEPHSSELLDLLDPDTRVNIRRLLQYPKDTAGSIMTTEFASVPSTYTVQQTLDYLRHV
                     ESSRETVYAIYVLDPTSKRLVKTITLRRLITGDPAAPVLSVARPGRLITATPLMDRED
                     VARLISKYDLLAVPVVDHGRVIGIVTVDDVIDAIVQENTEDVQKFGGMAAINEPYMEI
                     GFWQMMKKRGGWLCALFLSEMLTASAMQGYEGELEKAIVLTLFIPLIMSSGGNSGSQA
                     TSLLIRALALRELEIKDWWRVALREIPNGLALGAMLGLVGIVRITLWQYLGFYDYGEH
                     WSLIALTVGAALIGIVMFGSLTGSMLPFVLQRIGFDPASASAPFVATLVDVTGLVIYF
                     SVASLILRGTLL"
     gene            complement(3742..4935)
                     /gene="metC"
                     /locus_tag="VN968_24930"
     CDS             complement(3742..4935)
                     /gene="metC"
                     /locus_tag="VN968_24930"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HXL32510.1"
                     /translation="MNSPGNDGRSSPHKAETTLATAGRDTKAQKGFVNPPVVHGSTVL
                     YPTAEDLHAHRGEFQYGRRGTPTTKALQEALMALEGPQCAGVGIAPSGLSAVTTTLLA
                     VLKAGDHLLVCDNAYRPTRNFCDGLLARYGIETTYFDPLIGAGVGQLFKPNTKAILVE
                     APGSQSFEMPDIPAIAAVAHGRGALVIDDNTWATPLYHRSLEQGVDISIQAATKYIGG
                     HSDIMFGTISANAKAWPMIAEAIGLLGLCAGPDDVFLALRGLRTLAVRLAQHHRSALD
                     MARWLATRPEVVRVLHPALESDPGHAIWKRDFTGASGLFSIVLKPAPQQAVDALLDTV
                     KLFGMGYSWGGFESLVIPFDCADYRTATKWAPGGPALRLHIGLENVDDLKGDLDRGFA
                     AFNAA"
     gene            5269..6282
                     /locus_tag="VN968_24935"
     CDS             5269..6282
                     /locus_tag="VN968_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL32511.1"
                     /translation="MKRVFLAFTLALAAGLSTQATAQTLKTVKDRGMLSCGVSQGLPG
                     FSSPDDKGNWTGLDVDICRALAAAIFNDPTKIKFVPLSAKDRFTALQSGEIDVLSRNT
                     TWTLSRDTSLGANFTGVTYYDGQGFLVKKSLKVNSALELNSASVCVQTGTTTEQNLAD
                     YFKGNNMKYEVIAFGTNDEAVKAYESGRCDVFTNDVSGLYADRLKLSNPTDHAVLPEV
                     ISKEPLGPMVRHGDDQWFDIVKWVLFAMIDAEELGVTQKNVDDMAKSDKPELKRAFGT
                     DGNLGEQLGLTKDWMSRIIKAVGNYGESFDRNVGPGSRLGIARGLNQLWNKGGIQYAP
                     PIR"
     gene            6304..7512
                     /locus_tag="VN968_24940"
     CDS             6304..7512
                     /locus_tag="VN968_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842114.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL32512.1"
                     /translation="MTTIESRAPPSQLVSRLRRTLGGRAGWSGFALQILFVAALAWIG
                     YEIVANARANLQAQRITSGFGFLDQTAGFDVSQNLIPYSGSDTYTRVFLVGLLNTLVV
                     AIIGIFFATIIGFIVALGRLSPNWLLSRISGGYVELVRNLPLLFQILFWYRAVLATLP
                     NPRQGYALFDSVFLSNRGLVIPKPVANPGLEPVALAVLIAITGALWLRRYARKQLFEN
                     GRLIRVWPYVLGLLIGLPLLASLVFGTPVTFEFPTLRGFNFAGGSRIIPEFVALTLAL
                     STYTAAFIAEIVRAGILSVHKGQMEAGSSLGLQRGSTLRLIVVPQALRVILPPLTNQY
                     LNLTKNSSLAVAIGYPDLVSVFAGTTLSQTGQAIEIISITMGVYLLTSLVTSAIMSFY
                     GWRLGRSLGP"
     gene            7509..9059
                     /locus_tag="VN968_24945"
     CDS             7509..9059
                     /locus_tag="VN968_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HXL32513.1"
                     /translation="MSDISTPSFVRQELVPERPAPVKTTGFVGFLRTRLFNSPTNILV
                     TILGLLLLWFTVVPAIRFLLVDAVWTGKDRDACLAESAGHAVGACWPFIQAKITQLMY
                     GFYPDAELWRANLTFFLGALLLLPLLIPRLPAKGLNAGLFFIAFPVVAFFLLHGGGLK
                     GFGVSWTADFLSGFAAGMGDAGGKLMGVGAAGAVGLLGAVLALLGPVPLLLGKAVVLL
                     GSAVALLIWPLTWLRDQLQASSQPVWADFAATAAIVSILLFVLSGGIRTGWRALIVSL
                     AVFAGIGVVIAVMGLDRGGLPVVDTRLWGGLLVTLVVSVTGIVASMPVGIALALGRRA
                     SIPLIRIFSITFIEFWRGVPLITVLFFATYMLPLFLPGNFTVDGLVRALIGVALFAGA
                     YQAEVIRGGLQAIPRGQGEAASALGLSWGKTTGLIVMPQALRHVIPGLVNSFIALFKD
                     TSLVSIVALFDLLGSLQASFSDPVWSTPTTLFTGFAFVGLIYFTFCFGMSRYSLFVEN
                     RLNAHRRN"
     gene            9068..9808
                     /locus_tag="VN968_24950"
     CDS             9068..9808
                     /locus_tag="VN968_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HXL32514.1"
                     /translation="MSANSIVSINGLNKWFGDFHVLRDIDLDVGKSERIVICGPSGSG
                     KSTLIRCINALEEFQEGEIVVDGIELGPNLRRVDEVRREVGMVFQSFNLFPHLTVLEN
                     CTLAPIWVRNIPKKDAEAAAMKYLERVKIPHQASKFPGQMSGGQQQRVAIARALTMNP
                     KVMLFDEPTSALDPEMVKEVLDTMVDLANEGMTMLVVTHEMGFAREVANRIVFMDAGQ
                     IIEANTPQNFFANPQHARTKLFLSQILR"
     gene            9887..11812
                     /locus_tag="VN968_24955"
     CDS             9887..11812
                     /locus_tag="VN968_24955"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXL32515.1"
                     /translation="MNSLHVTGFLCLAFSVAFAVMASGAGADEAVPQTRLELTAANLA
                     SVVDPLMEEWIGKHQGPGAVVVVVTRDGPVFAKGYGFSDINAKKPFTADATLVRPGSI
                     SKLFTGIAVMQLVDAGRLDLDRDVNGYVDFAIPTPQGGVPVTLRRLLTHRAGFEEHAK
                     GLFSRDREPEPLGRWLAKNLPQRLFPNGDVEAYSNYGLALAGYIVERVSGEPYASYIR
                     RHILGPLGMSHSTFQQPLPDDLGPLMAKGYRRSDKPPLPFFETIAAAPAGALSATGAD
                     MGRFIRALMNGGELDGVRILPKARLDQMMAPGNATSAGYLGLVFFGTKIAGHDSIGHD
                     GGTMTFFSELKFFPEQGVGIFVSRDGIGEIKSFTEIPHPATAIAQRFLPRTTEWADAH
                     AASFPSEQRVAGIYQTSRRNESSFVRLTALLAQIVVKVDGDGHVRGLSAIWPFGNGAP
                     WKHVQRDLYEGPGARFAFVDDGDSDSYLATPGLRLQRVPWFLDLRWIAPAFVASTAMV
                     LLSLLAWPVAALWRYRRKRRWSEDRGDRGNFLAARLVVLVDAAVIGAAAVLFAMSSDF
                     TIFNDALDRWLLVLYAFAWLGVFGAVPTLLAATRFWRHGTGSRWSRVHHALIAASCVM
                     IAWFFLTFHIAGTTLIY"
     gene            11961..12518
                     /locus_tag="VN968_24960"
     CDS             11961..12518
                     /locus_tag="VN968_24960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HXL32516.1"
                     /translation="MLGFLFGFNARLGRLHYFLSTIALAVVMTAVCFAIASYAIQNTP
                     RGTDPSVGLIAWPVIVSILVFLWITFTLQSMRIRDIGWDPVCVIPAWIALLIVDKLVA
                     GKIPAWSLGPEHQGTIVGALINFGLILALTFWPSGEHEGGMPKSGETRRKPPELPRNQ
                     GTASAPAARIARATGTRAGFRRRAF"
     gene            complement(12563..13603)
                     /locus_tag="VN968_24965"
     CDS             complement(12563..13603)
                     /locus_tag="VN968_24965"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HXL32517.1"
                     /translation="MTIYNDVVEAIGNTPLIKLKRASEATGCTILGKAEFMNPGQSVK
                     DRAGKWMILEAEKRGDLKPGGLVVEATAGNTGIGLAVVASARGYRTLIVIPDTQSQEK
                     KDTLRLCGAELIEVPVLPYANPNNYQRVGKRLADQLRKSEPNGVLFADQWNNLDNAKA
                     HYESTGPEIWQQTGGKIDGFICSVGTGGTLAGTSRALKEKNKGIVVACADPHGFAMYE
                     LFKHGEAKSTPGDSITEGIGLGRVTPVIKTAEVDDAFLISDEEAVTVIYELLEHEGLC
                     LGGSTGINVAGAIRLAKQLGPGKTIVTILCDSGNRYQSKLFNPDFMRSKNLPVPQWLE
                     KRSKIDVPFEKV"
BASE COUNT         2422 a         4217 c         4321 g         2845 t
ORIGIN      
        1 attcatcgca ggccaggcgc tcgggctcgt ggtctacgtc cgcaatctgt attttatcat
       61 cgtcaacgga cggcaggcct cggcggacgg ttgagcgttg cgcgtgcacc gccgtcatcc
      121 ttgtgcgagc cgtcttcggc gagcctcgag ggatggcagc gaaacgcgca gccgccgccg
      181 gtccatgcgc cgcagcgccg agaccgggcc agcgagtgac acaatttatc gcggcagcgg
      241 atgcgcggca ggcagcgccg ccacatcgct ttcggtgctt cgataggcgg ccagctcgcc
      301 ggcggcgtcg aggacgggat aagccaccga gcagatatgc gagtggatgc gccgcaagtc
      361 acgcagcacg tcgagatgca gcgaggtggt ctcgatcgtc tccggtcggc cttcgcgcag
      421 acggtccaga tgccgctcgg tggcggcaag ctcggtattg cgcaacagtg ccttctccgc
      481 gagcagcttg cgcgcttcat tgacatcgcc cgacatgaag acgccgaaag cgatccgcag
      541 cgagtccatg attcgcttgt ggaatgccga cagttcttcc gccccctcgg gcgaaaactg
      601 gaacctacgc ttgatcttct tggtcgcgag ctcgctcaga ttcttgtcga tgatgtcgcc
      661 catatgctcg aggttgatgg caaaggagat gatctccatc gcccgctgtc cctcgcgctc
      721 gtcgaggctg ccgcgcgtca gcttggtcac ataaagcttg atcgcctcgt cgagactgtc
      781 gacggcattg tccatccgcg acacctgatc gaccagcgcg cgatcgttgg tcaccatcgc
      841 agccatcacc ttgcgcagca tgacctcgac gagatcgccc atgtgcaggg tttcacgcgc
      901 cgcgtccgcc aaggcgagcg agggggtttc cagcgcgctc tcatcgagat agcgcggccg
      961 cgatggatcg gtttcctgga cccggttcgg aagaagcctt ttcagcagcc gggccatgcc
     1021 gtcgagcaag ccgatgaaga tgatcgcggt cgccacgttg aacacgatat gaaattccgc
     1081 cgtgagcttg gcgaggtcgg gttgccaggc ccgcaaggtt tccgtgatcg ggtgcaggaa
     1141 cggcgcaacc agcagcacgc cgaccagccg attgatgaga ttgccgactg gcaggcggta
     1201 gctggcggga ttgtcgcggc gcgcgccttc gagaaccggg ttgatcgcgc tgccgagatt
     1261 ggcgccgagc acaagcgcca gcgatgcatc gggcgaaatg aattgggcgt aagccagcga
     1321 catcaccagc agaacgctgg ccacgctcga atgcacggcc caggtgacga tcgcgccgat
     1381 gacgatgcag agcacgggat cggcggtgat ggcgctcatg aacacacgca tgccgggcgc
     1441 attctccgca ggcgccagcg tgtcgagcag aatatgcagc gccagcagca tcaagccgag
     1501 cccgatcgag acgcggccga tgtccttgat gcgcgagcgc gcgccgctgc ggaaggcgac
     1561 caggccgatg ataaagagaa ccggcgcgat ggcggcgata ttgaaggata ggatttgaac
     1621 gatcagcgtg gtcccgacat tggcgccgag catgatggcc agcgccggga ccaggctgac
     1681 caggccttcg gccgtgaagg agctggtaat cagggcggtc gcggtgctgc tctgcaacag
     1741 ggcggtcagg ccgatacccg ctgcgaaggc cttgaagcga ttgttcagcg ctttcgccag
     1801 aagcagacgg agatcgggac cgaaggcgcg caggatgccg ctgtgaacca tgtgcaggcc
     1861 ccacagcagc agcgccacgc cgcccatcag gtcaagaaga accacacttc ccatgagttt
     1921 ccatttccgg atttgagtcg aacgatcagg ctatcgccaa acgacggcgg atcaatcgtt
     1981 tatttcgctg gaacagcacg gaaatgcagc gacagccgcc gctcggttga gcacgggcgg
     2041 gctcaccacc cgcgcgccgc gcggcgcgga aggccgccga gccgcgcgcc gtcaggggca
     2101 ggaaacgttc tttcgccgac gactcggaaa gcgcgaagtc accgtcaatc agccccggca
     2161 cgcgctgggt gagatcactg gacttgcccc accaatacca tccaacgggg cctgccgttc
     2221 cgctgcgatt tcctttattt acagggcaag gctcaaggct tatcagccgt ccgtcgtcag
     2281 cccggagatg tgaatcatga ccgaccttga cctccaggat gatccggcgg cgaaggacga
     2341 caacatggtc gatccggcgt tcctcgccgc cagcatgatc gaagaacgcg cgcccgacat
     2401 tgtcgaagcc cttaacgaca tgcctccgga actcgccgtc gcggttttgc ttcatctgcc
     2461 gcccgatcgc gccattgaag tcctcgacca gccgggactg gatcgcgaac cggaactctt
     2521 cacgctgatg ccgcgggaag cggccgcaaa gctgctggcc ggtgtgtcgg ccgaccgcgt
     2581 cgccgacatc tttcggcagg tcaaggagcc gcacagctca gagctgctcg atctgctgga
     2641 ccccgacacc agggtcaaca tccgccgcct gttgcaatat ccgaaggaca ccgccggatc
     2701 gatcatgacg acggagttcg ccagcgttcc ctcgacctac acggtccagc agaccctcga
     2761 ctatcttcgt cacgtggaaa gcagccggga aaccgtctac gcgatctacg tgctggatcc
     2821 gaccagcaag aggctggtca agacgatcac tctgcggcgg ctgatcacgg gagatccggc
     2881 agcaccggtg ctctcggtgg cccgtccagg ccgtctgatt accgccactc cgctgatgga
     2941 ccgggaagat gtcgcgcgcc tgatctcgaa gtacgacctg ctcgcggtgc cggtcgtcga
     3001 ccacggtcgc gtcattggga tcgtcacggt cgacgacgtc atcgacgcca tcgtgcagga
     3061 aaacaccgag gacgtgcaga agttcggcgg catggccgcc atcaacgagc cgtacatgga
     3121 aatcggcttc tggcagatga tgaagaagcg cggcggctgg ttgtgcgcgt tgttcctgtc
     3181 cgaaatgctg acggcatcgg cgatgcaggg ctacgagggc gagctcgaga aggccatcgt
     3241 gctgacgctg ttcatcccgc tgatcatgag ctcgggaggc aattccggat cgcaggcgac
     3301 atcccttctg atacgcgcat tggccttgcg tgaacttgaa atcaaggact ggtggcgcgt
     3361 ggcgctgcgg gaaatcccga acggactggc gcttggggcg atgctgggcc ttgtcggcat
     3421 cgtccgcatc acgctctggc aatatctcgg cttctacgac tacggcgagc actggtcgct
     3481 gatcgcgcta acggtcggcg ccgccctgat cggcatcgtc atgttcggct cgctgaccgg
     3541 atccatgctc ccgttcgtcc tgcagcggat cggcttcgac ccggccagcg cttcggcccc
     3601 gttcgtggcg accctcgtgg acgtaaccgg tttggtgatc tatttcagcg tcgcttcgct
     3661 gatcctgcgc ggcaccctgc tgtagcagtt ccttcgtccg tcgatgcgcg ccgccggctc
     3721 ccggcgatga gtgcggggac gctaggcagc gttgaacgcc gcaaagccac gatcgaggtc
     3781 gcctttgaga tcgtcgacgt tctccagccc gatgtggagc cgcagggccg gtccacccgg
     3841 cgcccatttg gtcgccgtac gatagtccgc gcagtcgaac ggaatcacaa ggctctcgaa
     3901 gccgccccat gaatagccca tgccaaacag cttgacggtg tcgagcaagg cgtcgacggc
     3961 ctgttgcggc gcgggcttca ggacgatgct gaacaggccg gaggcgccgg taaaatcccg
     4021 tttccagatc gcgtggccgg gatcgctttc gagcgcgggg tgcagcaccc tgacgacctc
     4081 gggccgcgtc gccagccaac gcgccatatc gagcgccgat cgatgatgct gcgccagccg
     4141 caccgcgagc gtgcgcaggc cgcgcagcgc gagaaacaca tcgtccggcc ctgcgcagag
     4201 gccgagcagc ccgatcgcct cggcaatcat cggccacgct ttcgcattcg ccgagatcgt
     4261 gccgaacatg atgtcggaat gtccgccgat atatttggtc gccgcctgaa tgctgatgtc
     4321 gacgccttgc tccagcgaac ggtgatacaa cggcgtcgcc caggtgttgt cgtcgatgac
     4381 aagcgcgccg cggccatgcg cgacagcggc gatcgcggga atgtcgggca tttcgaagga
     4441 ttgcgaaccg ggcgcctcga ccagtatcgc cttggtgttg ggcttgaaca attggccgac
     4501 accggcgccg atcagcggat cgaaataggt cgtctcgatg ccgtagcgtg cgagcaatcc
     4561 gtcgcagaaa tttcgtgtcg ggcgataagc gttgtcgcag accagaagat gatcgccggc
     4621 cttcagcacc gcgagcaggg tcgtggtgac ggccgacagc cccgagggcg caatgccaac
     4681 gcccgcgcat tgcgggcctt cgagagccat cagcgcttcc tgcagcgcct tggtggtcgg
     4741 cgttccgcgg cggccatact gaaactcgcc gcgatgggcg tgcagatctt ccgcggtcgg
     4801 ataaagcacg gtggagccgt ggacgaccgg gggattgacg aagccttttt gcgccttggt
     4861 gtcgcgcccc gccgtcgcca gcgtggtttc tgccttgtgc gggctcgacc gtccgtcatt
     4921 gcctggagaa ttcatgggtc cgccgccacc gaaagacttt ttgccgcagc cgcgacgttg
     4981 ctctgaaagc ccaccggacg gcgggttcgg gctgggttgg ttaataacag gacatggaag
     5041 atggccgtca accccttgac ccggcccgcc agtcgttctg tgatgtgtgg gagctattca
     5101 gtcgcagcac gttgaggggc ctgccgcgac ctttgatccg cttcttgacc agactgcgcg
     5161 ctaaactgct tggcagacgg cgtttacagt aacggatcgc cacggttggc ccatcggcgt
     5221 atgcgatgta agcagacgtt cccggaaaga cccttgaaag gctgtgtcat gaaacgcgta
     5281 ttcctggctt ttacccttgc tcttgccgcc ggcctctcga cccaggccac cgcgcaaacg
     5341 ctgaagacgg tcaaggaccg cggcatgtta tcctgcggtg tgagccaggg tttgccgggc
     5401 ttttcgtccc ccgacgacaa gggcaactgg accgggctcg acgtcgatat ctgtcgcgcg
     5461 cttgcggccg ccatcttcaa cgatccgacc aagatcaagt tcgtgccgtt gtcggccaag
     5521 gatcgcttca cggcactgca gtccggcgaa attgatgtac tttcgcgcaa tacaacctgg
     5581 acgttgtccc gcgacacctc gctcggcgcc aattttaccg gcgtgaccta ttatgacggc
     5641 cagggttttc tggtgaaaaa atccctcaag gtgaactctg cgctggaact gaacagcgca
     5701 tcggtttgcg tgcaaaccgg caccacgacg gagcagaatc tcgccgacta cttcaagggc
     5761 aacaacatga agtatgaggt gattgcgttc ggcaccaacg acgaagccgt caaggcctac
     5821 gagtccggac gctgtgacgt cttcaccaac gatgtgtcgg ggctttatgc cgatcgtttg
     5881 aagctttcaa atcccaccga tcacgccgtg cttccagagg tgatctcgaa agagccgctc
     5941 ggcccgatgg tgcgtcatgg cgacgaccaa tggttcgata tcgtgaaatg ggtgctgttt
     6001 gcgatgatcg acgcggaaga actcggcgtc acccagaaga acgtcgacga catggcgaaa
     6061 tcggacaagc ccgagctgaa acgggcgttc ggcaccgatg gcaatctcgg cgaacagctt
     6121 gggcttacca aggactggat gtcgcggatc atcaaggcgg tcggcaatta cggcgaatcg
     6181 ttcgatcgca atgtcggccc cggctcaagg ctaggaattg cccgtgggct caatcagcta
     6241 tggaacaagg gaggaattca gtacgcgccg ccgatccgct aatcgccgtc cgcctcgacg
     6301 gcgatgacca ccatcgaatc acgagcaccc ccgtcgcagc tggtctccag gctgcgacgc
     6361 acgctcggcg ggcgcgcggg ctggagcgga ttcgcgcttc agattttgtt tgtcgccgcc
     6421 ctggcctgga ttggctatga gatcgtggcc aacgcacgcg ccaatctgca ggcgcagcgg
     6481 atcacgtccg gcttcggctt tctcgatcaa accgcgggct tcgacgtcag ccagaacctg
     6541 attccctatt cgggatcgga tacctacact cgcgtgttcc tggtcggctt gctgaacacg
     6601 ctggtggtcg ccatcatcgg cattttcttc gccaccatca tcgggttcat cgtcgcgctg
     6661 ggccggctgt cgccgaattg gctgttgtcg cggatctccg gcggttacgt tgaactggtg
     6721 cgcaacctgc cgctattgtt ccagatcctg ttctggtatc gggcggtgct cgcgacgttg
     6781 ccgaacccgc ggcaaggcta cgcgctgttc gacagcgttt ttctcagcaa tcgcgggctc
     6841 gtgattccga agccggtcgc aaatcctggc ctggagccgg tcgcgcttgc cgtactgatt
     6901 gcaatcaccg gcgcgctgtg gctgcggcgc tacgcgcgca agcaactgtt cgaaaacggc
     6961 agactgatta gggtctggcc ctatgtgctc gggttgctga tcgggttgcc cttgctggcg
     7021 tcgctggtgt tcggcacgcc cgtcaccttc gaatttccca cgctcagggg attcaatttt
     7081 gccggtggct cacgcatcat tccggaattc gtggcgctaa cgctggcgct ttcgacctac
     7141 acggcggcct tcatcgccga gatcgtacgt gctggcatcc tgtcggttca caaggggcaa
     7201 atggaagccg ggtcgtcgct cgggctacag cgcggttcga cgctgcgcct gatcgtggtg
     7261 ccgcaggcgt tgcgcgtcat cctgccgccg ctcaccaacc agtatctcaa tctcaccaaa
     7321 aactcgtcat tggcggtggc gatcggttat cccgatctgg tttcggtgtt cgccgggacg
     7381 acgctcagcc agaccggaca ggcgatcgag atcatctcga tcaccatggg ggtctatctc
     7441 ctgacctcgc tggtcaccag cgcgatcatg agtttctacg gatggcggct cggccggagt
     7501 ctcggcccat gagcgacatc agcacaccat ctttcgtccg tcaggaactg gttcccgagc
     7561 ggcccgctcc ggtcaagacc accggctttg tcggtttcct gcgtacgcgc ctgttcaatt
     7621 caccgaccaa tattctggtg acgattctag ggctgctgct gctgtggttt accgtcgttc
     7681 cggcgatcag gtttcttctg gtcgatgcgg tctggaccgg caaggaccgc gacgcctgtc
     7741 ttgcagagag tgccgggcac gcggtcggcg cgtgctggcc ctttatccag gcgaaaatca
     7801 cccagctcat gtacggattc tacccggatg ccgagctgtg gcgggcgaac ctgacatttt
     7861 tccttggcgc ccttttgctg ttgccgctct tgattccgcg actgccggcc aaggggctta
     7921 atgccggcct gttcttcatc gcgtttcccg tagtcgcctt ctttctgctg catggcggcg
     7981 gtttgaaggg ctttggtgtc agctggaccg cggatttcct gtcgggtttt gccgccggca
     8041 tgggcgacgc gggcggcaag ctgatgggcg tcggtgccgc cggcgcagtc gggctgctcg
     8101 gagcggtgtt ggcgctgctt ggaccggtgc cgttgctgct tggcaaggcc gtggttctct
     8161 tgggcagcgc cgtcgcattg ctgatttggc cgctgacctg gcttcgcgat caacttcagg
     8221 cctcaagcca gccggtctgg gccgacttcg cggccaccgc cgccatcgtc tcgatcctgc
     8281 tgttcgttct gagtggcggc atccgcaccg gatggcgcgc gttgatcgtc agccttgcgg
     8341 tgtttgccgg catcggcgtc gtgatcgctg tcatgggcct cgaccgcggc ggactgccgg
     8401 tggtcgatac ccggctgtgg ggcgggcttc tggtgacgct ggtggtatca gtcaccggca
     8461 ttgtggcctc gatgccggtt ggtatcgcat tggccttggg ccgtcgcgcg agcattccgt
     8521 tgatccggat tttctcgatc acctttatcg agttctggcg cggcgtgccg ctgatcacgg
     8581 tgttgttttt cgcaacctac atgctgccac tgttcctgcc tggaaatttc acggtggacg
     8641 gcctggtccg cgccctgatc ggggtcgcgc tgttcgccgg cgcttaccag gccgaagtca
     8701 tccgtggcgg attgcaggcg atcccgcgcg ggcaaggcga ggcggcgagc gcgctgggcc
     8761 tgtcctgggg gaaaacgacc ggcctgatcg tgatgccgca ggcgctgcgc cacgtcattc
     8821 ccggtctcgt caacagcttc attgccctgt tcaaggacac ctcgctggtc tcgattgtcg
     8881 cgctcttcga tctgctgggc agtctgcagg cttccttcag cgatccggtg tggtcgacgc
     8941 cgacgacgct gttcaccggg ttcgcgtttg tcggactcat ttacttcacc ttctgctttg
     9001 gaatgtcgcg ctattccctg ttcgtcgaga acaggctgaa cgcacatcgc cgtaactgag
     9061 taatcacatg agcgcaaact ccattgtcag catcaacggc ctcaacaagt ggttcggcga
     9121 ttttcatgtg ctgcgcgaca tcgatcttga tgtcggcaaa agcgagcgca tcgtgatctg
     9181 cggtccttcg ggctcgggca aatcgaccct gatccgctgc atcaatgcgc tggaggagtt
     9241 tcaggaaggc gagatcgtcg tcgacggcat cgagcttggt ccgaatttgc ggcgcgtcga
     9301 tgaagtgcgg cgcgaggtcg gcatggtgtt ccagagtttc aatctgtttc cgcatttgac
     9361 cgtattggag aactgcacgc tggcgccgat ctgggtgcgc aatattccga agaaggatgc
     9421 cgaggcggcc gccatgaaat acctcgagcg ggtgaaaatt ccgcaccagg caagcaagtt
     9481 tccaggccag atgtccggcg gccagcaaca gcgggttgcg atcgctcgcg ccctgaccat
     9541 gaacccgaag gtaatgctgt tcgacgagcc gacctcggcg cttgatcccg aaatggtcaa
     9601 ggaagtcctc gacaccatgg tcgatcttgc caatgaaggc atgaccatgc tggtcgtcac
     9661 ccacgaaatg ggatttgcca gggaagtcgc gaaccgcatc gtcttcatgg acgccgggca
     9721 gatcatcgag gccaacacgc ctcaaaactt cttcgccaac ccgcagcacg cacggaccaa
     9781 gctgtttttg agtcagattt tgcggtgaat gaggcgagtg gcgaatagag aatcgggaaa
     9841 gttttattcg ctacccgtga ctcgcgactc gtcgtgggga ttttccatga actcccttca
     9901 tgtcaccggc ttcttgtgct tggctttctc ggtcgctttc gccgtgatgg catctggcgc
     9961 aggcgccgac gaagcggtgc cgcagacgcg gcttgagctg acagcggcca accttgccag
    10021 cgtggtcgat ccgctgatgg aggagtggat cggcaaacac cagggccctg gtgccgtggt
    10081 cgtggtggtg acgcgcgatg gcccggtgtt cgccaagggc tatggctttt cggacatcaa
    10141 tgccaaaaag ccattcaccg ccgatgcgac cctggtgcgg ccgggatcga tctcgaagct
    10201 tttcaccggc atcgccgtca tgcagctcgt ggacgccggc cggctcgatc tcgaccgcga
    10261 cgtcaacggt tatgtcgatt tcgcgattcc gacgccgcaa ggcggcgttc ccgtcacact
    10321 gcgccgtctg ttgacgcatc gggccggttt cgaggaacat gcgaaaggac tgttctcgag
    10381 ggaccgcgag cctgaaccgc ttgggcgttg gcttgcgaaa aatctgccgc aacgtctgtt
    10441 tcccaacggc gatgtcgagg cctattccaa ttatggcctc gcgctcgccg gctatatcgt
    10501 cgaacgcgtt tcgggtgagc cctacgcctc ctatattcgg cggcatattc tgggtccgct
    10561 cggcatgagt cactcgacct tccagcagcc gttgccggac gatcttgggc cgctgatggc
    10621 gaaaggttac cgcagatcgg acaagccgcc gctgccgttt ttcgagacca tcgctgccgc
    10681 gccggccggc gcgctttcgg cgacgggtgc ggacatgggc cgctttatcc gcgcgctgat
    10741 gaatggcggc gagctcgatg gcgttcgcat cctgcccaag gcgcggctcg accagatgat
    10801 ggcgccgggc aacgcgacat cggccgggta cctcggtctg gtgttcttcg gaaccaaaat
    10861 cgcgggccat gactcgatcg ggcacgacgg cggaaccatg acattcttca gcgagttgaa
    10921 gtttttcccc gagcagggcg tcggaatttt tgtgtcacgc gacggaattg gcgaaatcaa
    10981 gagtttcacg gagatacccc atcccgcgac ggccattgcc cagcgttttc tgcccaggac
    11041 gacggaatgg gccgatgcac acgccgccag cttcccaagc gagcagcgtg tggccggcat
    11101 ctatcagacg agccggcgca acgagtcgtc ttttgtgaga ttgactgcgc tgctggcgca
    11161 gattgtcgtc aaggtcgacg gggacgggca cgtgcgaggg ttatctgcga tctggccgtt
    11221 cggtaacggc gcgccatgga agcacgttca acgcgatctc tacgaagggc caggcgcgcg
    11281 cttcgccttt gtcgatgacg gcgactccga ctcttacctg gccacaccgg gtcttcgtct
    11341 gcagcgcgtt ccctggtttc tcgatctgcg gtggatcgcg ccggcgtttg tcgcgagcac
    11401 agcgatggtt ctcctgtcgc tgctggcctg gccggttgcc gcgctatggc gttaccggcg
    11461 caagaggcgg tggagcgagg accgcggcga ccggggtaac tttctcgccg ccaggctggt
    11521 cgtgctggtc gatgcggccg tcattggcgc tgccgcggtg ctttttgcga tgagttcaga
    11581 cttcaccatt ttcaatgacg ctctcgatcg ctggctgctg gtgctctatg ccttcgcgtg
    11641 gctcggcgtt ttcggcgcgg tgccgacgct gttggccgcc acccgattct ggcgccatgg
    11701 caccggcagc cgatggtcgc gtgttcatca cgcgctgatt gccgccagct gcgtgatgat
    11761 cgcgtggttc ttcctgacat tccacatcgc ggggacgacg ttgatttatt agaccccgtt
    11821 ggatgcggtt ttgtcgcttt ccgcagcacg ccgattttcg aaatcagcga aagcgactgg
    11881 aaaccataac ctgccgaggc cgtagccggt tcaccgactg gaaagcaatt tgtggccttt
    11941 tatctcaaaa ggaggcgcca atgttgggtt tcctgttcgg tttcaatgcc cggctcggcc
    12001 ggctgcatta cttcctcagc acgattgccc tggctgtggt gatgaccgcg gtctgtttcg
    12061 ccattgcgag ctatgcgatc cagaacacgc ccaggggaac ggatccatcc gtcggcctga
    12121 ttgcgtggcc ggtgatcgtc tccatcctcg tgttcttgtg gatcaccttc acgctgcagt
    12181 ccatgcgtat cagggatatc ggatgggacc ctgtctgcgt catcccggcg tggatcgcgc
    12241 tcctcatcgt cgataagctt gttgcaggca agatccccgc gtggtcgctg ggtcctgaac
    12301 atcagggaac gatcgtcggc gcgttgatca acttcggact gattttggct ctgacgtttt
    12361 ggccgagcgg cgaacacgaa ggtgggatgc cgaagtctgg cgagacgcgc cggaagccac
    12421 ctgagctacc gcgcaatcaa ggtacagcgt cggcgcctgc ggcacgcatc gcgcgggcga
    12481 cgggaacgcg agcgggattc cgccgccgcg cattttgaat gtcgttggcg acagcgattg
    12541 ggctcgctgc gccgatgccc aatcacacct tctcgaacgg cacatcgatc ttgctgcgct
    12601 tctccagcca ttgcggtacg ggcaggttct tcgagcgcat gaagtccgga ttgaacagct
    12661 ttgattgata gcgattgccg gaatcgcaga ggatggtgac aatggtcttg ccggggccaa
    12721 gctgtttggc gaggcggatc gcgccggcca cgttgatgcc ggtcgagccg ccgaggcaca
    12781 ggccttcatg ctccagcaat tcgtagatca cggtcacggc ttcctcgtcc gaaatcagga
    12841 aggcatcgtc gacctcagcg gtcttgatta cgggcgtgac gcggccgagg cctatgcctt
    12901 cggtgatcga atcgcccgga gtcgatttgg cctcgccgtg cttgaacagt tcatacatcg
    12961 cgaagccatg cggatcggcg caggcgacga cgatgccctt gttcttttcc ttcagagccc
    13021 ggctggtgcc ggcgagcgtg ccgccggtgc cgaccgagca gatgaatccg tcgatcttgc
    13081 cgccggtctg ctgccagatt tccgggccgg tcgattcgta atgtgccttc gcgttgtcca
    13141 gattgttcca ttgatcggcg aacagcacgc cgttcggctc gctcttgcgc aactggtcgg
    13201 ccagcctttt tccgacacgc tggtaattgt tcgggttggc gtagggcagc accggaactt
    13261 cgatcaattc ggcgccgcag agccgcaacg tatccttctt ttcctggctc tgggtgtccg
    13321 gaatcacgat caaggtccgg tagccgcgcg cgctcgccac caccgcaaga ccgatgccgg
    13381 tattgcccgc ggtcgcttcc accaccaggc cgcccggctt gaggtcaccg cgtttctcgg
    13441 cttcgaggat catccatttg ccggcgcggt ccttcaccga ctgcccggga ttcatgaact
    13501 cggccttgcc gagaatggta cagccggtcg cctcggaagc ccgcttcagt ttgataagcg
    13561 gcgtattgcc gatcgcttcg acgacgtcgt tgtaaatggt catgtctgtg aaatctttgc
    13621 cggtcggttg tcaaatccgc tacgacccta gagcgcgctt ggcaaaagtg gaagcgtttt
    13681 gcgctccgtg gtgcagtgcg aactactctg acttcgcgaa caccacctgg cggacgtcga
    13741 tgtttcccga caggaagccg gcctcgcaat aagcgaggta gtattcccac agccgccgga
    13801 accgg
//
LOCUS       DATNOI010000614         2222 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1474969,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000614 DATNOI010000000
VERSION     DATNOI010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474969"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VN968_24970"
     CDS             <1..391
                     /locus_tag="VN968_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HXL32518.1"
                     /translation="NLSFNPISALTHATLVDICQFAETRELAATMMKEAQDIAQKLGV
                     TFRVTIDKRIAGAEAVGAHKTSMLQDVEAGRSLETEALIGSILEMAKLTNTAAPSIEA
                     VYALVKLLNKVMLLEGGGVKVEKMKAA"
     gene            complement(479..1249)
                     /locus_tag="VN968_24975"
     CDS             complement(479..1249)
                     /locus_tag="VN968_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HXL32519.1"
                     /translation="MRRPVVGVIGSAYRIEDRFATQLVGERNLRAVSEVAGALPLMFA
                     AAPEITDIGALLDAVDGIVLTGARANVHPARFGSEPDSRHEPFDLHRDDLALALSEAC
                     VARGIPLFGICRGLQEMNVAFGGSLHPEIRELPGRMNHRMPRLESGEIHPDATVVFAD
                     RHDVRLVPGGVFARLLGCETIRVNSLHGQGILEPGKRVVVEGVAEDGTIEAIRIADAP
                     GFALGVQWHAEYDPQRNPINRALFRAFGEALLAHRRAN"
     gene            complement(1345..2037)
                     /locus_tag="VN968_24980"
     CDS             complement(1345..2037)
                     /locus_tag="VN968_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32520.1"
                     /translation="MNFRWLGPLVLLAVLVIGDQIRINRPGHKYRLTVEVETPEGTKS
                     ASGVLAVRPDRGYSRGALTRTMGDAVLLDLGGGKNLIALLAHVDKTVELDGMNYLALR
                     AYNAAGRKVSFNDMSRMTGPVPVQGALIPVLVTFAVSGDPATARVVPPDGTEAALGKG
                     FHLRGISTEVVPNGFWPLDFGGPFGEPVTRGVEAKLPWWSRPDDPAGKALQAAGLQGG
                     EAIDPKQVFTRK"
BASE COUNT          425 a          740 c          663 g          394 t
ORIGIN      
        1 caatctctca ttcaacccga tctcggcgtt gacccacgcc acgctggttg acatttgcca
       61 gttcgccgaa acgcgcgaac tggccgccac catgatgaag gaagcgcagg acatcgcgca
      121 gaaactcggc gtcaccttcc gtgtcaccat cgacaagcgc atcgccggcg ccgaagcggt
      181 cggcgcgcac aagacatcca tgctgcagga cgtggaagcc ggccgctcgt tggaaaccga
      241 ggcgctgatc ggctcaattc tggaaatggc caaactgacc aatacggcgg cgccctcgat
      301 cgaggccgtc tacgcgctgg tgaagcttct caacaaggtc atgctgctgg agggcggcgg
      361 cgtgaaggtg gagaagatga aggcggcttg agcggatttt acgccatttc ggatgcactc
      421 ggcgcttctc atgacgggaa gcgccgtcgc gccgaacctt ggcgctccgt ctcgccagct
      481 agtttgcccg gcggtgcgcc aacaacgcct cgccgaacgc ccgaaacaat gctcggttga
      541 tgggattgcg ctgcgggtcg tattcggcat gccattgcac gccgagcgca aagcccggcg
      601 cgtcggcaat ccggatcgcc tcgatcgtgc cgtcctccgc cacgccctcg acgaccaccc
      661 gttttcccgg ttcgaggatg ccctggccgt gcagggaatt gacgcggatg gtctcgcaac
      721 cgagcaaccg ggcaaagacc ccgccgggaa cgagacggac gtcgtgacgg tcggcgaaga
      781 ccacagtggc gtccggatgg atttcgccgc tttccaggcg cggcatgcga tggttcatgc
      841 gtccgggcaa ttcgcggatt tccggatgca gggagccgcc gaacgccacg ttcatctcct
      901 gcaagccgcg gcagataccg aacaggggta tgccgcgcgc gacgcaagct tccgacagtg
      961 ccagcgccag gtcgtcacga tgcaggtcga agggttcgtg ccgcgaatcc ggttcgctgc
     1021 caaaacgcgc cgggtgaacg ttggcgcgag ctccggtcag cactatgcca tcgaccgcat
     1081 ccagcagcgc gccgatatcg gtgatctcgg gggcagcggc aaacatcagc ggcagcgcgc
     1141 ccgcgacttc ggacaccgcg cgaaggtttc gctctccgac cagctgtgtc gcaaatcgat
     1201 cttcgatgcg ataggcgctt ccgatcacac cgaccaccgg cctcctcatc gtctgctatc
     1261 caatctcccg ccgcaaatat ggccgatcat gaccgctggc ccgcaccgga tcaatatctc
     1321 gcggcccggt gcgtggctgc attgttattt ccgcgtgaag acctgttttg ggtcgatcgc
     1381 ctcgccgcct tgcaaacccg cggcctgaag ggccttcccc gcgggatcgt caggccgact
     1441 ccaccacggc aatttagcct ctaccccacg tgtcaccggc tcgccgaacg ggccgccgaa
     1501 atcgagcggc cagaagccgt tcgggaccac ctctgtcgag atgccacgga gatgaaatcc
     1561 cttgcctagc gcggcttccg ttccatccgg cggcactacg cgcgcggtcg cgggatcgcc
     1621 tgaaacggcg aaagtcacca gcaccgggat cagcgcgcct tgcacgggca ccggcccggt
     1681 catccggctc atgtcgttga atgacacttt gcgaccagcc gcgttgtagg cgcgcaaggc
     1741 cagatagttc ataccatcga gctcgaccgt cttgtcgaca tgcgcgagca gcgcaatcag
     1801 gtttttgccg ccgccaagat caagcaggac cgcatcaccc atggtgcggg tgagcgcacc
     1861 gcggctgtag cctctgtcag gtcgaaccgc caggactcca gaggccgatt ttgtgccttc
     1921 aggcgtttcc acctcgaccg tcagccggta tttgtgaccg ggccggttga tccgaatctg
     1981 atcgccgatc accagcaccg ccaatagtac gagcggcccg agccacctaa agttcatgca
     2041 tccgccctcg cccgcgatcc gattccgctc acgctttgca caacgaacat gccagcgtca
     2101 aaaccggcgg gcttctccgc ttgatcccgg gcgcgtttga cgaaagagtg ctgtcggaaa
     2161 ccgttgcaag aaggaatcaa cttgacagtc cggactggcg atcgtcttgc ccgcaacgat
     2221 ct
//
LOCUS       DATNOI010000615         2729 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_937020,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000615 DATNOI010000000
VERSION     DATNOI010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937020"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1576
                     /gene="purF"
                     /locus_tag="VN968_24985"
     CDS             77..1576
                     /gene="purF"
                     /locus_tag="VN968_24985"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXL32521.1"
                     /translation="MRDSDFQDSGLQDSGLQDSDLEGDTLREECGVFGIFGHPDAAAI
                     TALGLHALQHRGQEAAGIVSFERGRFHSERRLGLVGDTFSRREVIDRLPGTSAIGHVR
                     YSTTGETILRNVQPLFAELNAGGFAVGHNGNLTNGLTLRRELVRNGAMMQSTTDTEVI
                     LHLVAQSKRNRFIDRFIEALRAIEGAYSLVSLTNKKLIGARDPLGIRPLVLGELDGCP
                     ILASETCALDIIGAKYVRDVEPGEVIVFDEQGAQSHKPFPPKPPRPCIFEYIYFSRPD
                     SIVGGRSVYEVRKAFGAQLARESHVEVDVVVPVPDSGVPAAVGYSQHSGVPFELGIIR
                     NHYVGRTFIQPTQSVRELGVRMKHNANRAAIEGKRIILIDDSLVRGTTSKKIVRMMRD
                     AGAREVHFRLASPPIIHPDYYGIDLPDRGGLLAATHNLEEMRDIIGADSLAFLSIDGM
                     YRAMGEPGRDPLNPKFADHCFTGVYPTHLTDHTQHEPQPRQLSLLAEAS"
     gene            1745..2485
                     /locus_tag="VN968_24990"
     CDS             1745..2485
                     /locus_tag="VN968_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL32522.1"
                     /translation="MTTPLASRIALVTGASRGIGYATARALAKAGAHVVAVARTQGGL
                     EELDDEIRKGGGAATLVPLNLTDFDGIARLGAALHERHGKLDILVGNAGVAGPSSPLG
                     HIELKPWSDVMAINVSANFQLIRCMEPLLKKSDAGRAVFVTSGAASKASAYLGPYAAS
                     KAALETLVRVWARETAATAVRVNLFDPGPIRTRMRATVFPGEDPLTLDTPDQAAEFIV
                     PMCAPDWTETGKFYDYKTRTLMSFRGPA"
BASE COUNT          503 a          934 c          817 g          475 t
ORIGIN      
        1 acagagccct tccgatgaag ccgctgaaag cgacctgaat ctcggcgacc tgaacctcga
       61 cccaaccaat ctgaacctgc gagattccga cttccaagat tccggcttgc aagattccgg
      121 cttgcaggat tccgatttgg aaggcgacac gctgcgcgag gaatgcggcg tgttcggcat
      181 cttcggccac cccgatgccg ccgcgatcac cgcactcgga ttgcacgctc tgcagcatcg
      241 cggccaggag gccgcgggca tcgtttcctt cgaacgaggc cgatttcact ccgagaggcg
      301 gctcggcctc gtcggcgaca ccttctcccg ccgcgaggtg atcgaccggc tgccgggcac
      361 ctcggcgatc ggccacgtcc gctactccac caccggcgag accatcctgc gcaacgtcca
      421 gccgctgttc gccgaactca atgccggcgg cttcgcggtc ggccataacg gcaatctcac
      481 caacgggctg accttgcgcc gcgaactggt gcgcaacggc gccatgatgc aatccaccac
      541 cgacaccgag gtcatcctgc acctggtggc gcagtccaag cgcaaccgct tcatcgaccg
      601 cttcatcgag gccctgcgcg cgatcgaggg cgcctactcg ctggtgtcgc tgaccaacaa
      661 gaagctgatc ggcgcgcgcg atccgctcgg cattcgcccg ctggtgctgg gcgaactcga
      721 cggctgcccg atcctggcgt cggagacctg cgcgctcgac atcatcggcg cgaaatatgt
      781 ccgcgacgtc gagcccggcg aagtcatcgt gttcgacgag cagggcgcgc aaagccataa
      841 accgtttccg ccaaagccgc cgcggccctg catcttcgag tacatttact tttcgcggcc
      901 ggattccatc gtcggcgggc ggtccgtgta tgaagtccgc aaggcgtttg gcgcgcagct
      961 cgcgcgtgaa agccatgtcg aggtcgatgt cgtggtgccg gtgccggatt ccggcgtgcc
     1021 tgccgccgtc ggctacagtc agcattccgg cgtgccgttc gagcttggca tcatccgcaa
     1081 ccattatgtc ggccgcacct tcattcagcc gacccagagc gtgcgcgaac tcggggtgcg
     1141 catgaagcat aatgccaacc gcgccgcgat cgaaggcaag cgcatcatcc tgattgacga
     1201 ttcgctggtg cgcggcacca cctcgaaaaa gatcgtccgc atgatgcgcg atgccggcgc
     1261 cagggaagtc cacttccgcc tcgcctcgcc gccgatcatt catcccgatt actacggcat
     1321 cgatctgccc gaccgtggcg gattgctggc ggcgacgcat aacctcgaag agatgcgcga
     1381 catcatcggc gccgattcgc tggcgttcct gtcgatcgac ggcatgtatc gcgccatggg
     1441 cgagcccggc cgcgatcccc tgaatccgaa attcgcggac cattgcttca ccggcgtcta
     1501 tcccacgcac ctcaccgatc acacccagca cgagccgcag ccgcggcaat tgtcgctgct
     1561 ggcggaggcg agctgaggcc aagcgttcta ttcctaattc gtcatggccg ggcttgtccc
     1621 ggccatccac gtctgtaaaa cccgcagcgc aagcaagacg tggatgcccg gcatcgcaga
     1681 caagtttacg cagtctgcgc aaagcagact gctatggccg ggcatgacgg agaagtctac
     1741 ttcgatgaca acacccctcg cctcccgtat cgctctcgtc accggcgcct cgcgcggcat
     1801 cggctatgcc accgcgcgcg cgctggccaa agccggcgcg cacgtcgttg ccgtggcgcg
     1861 aacccaaggc ggcctcgaag aactcgacga cgaaatccgc aagggtggcg gagctgcgac
     1921 cctggtgccg ctcaatctga ccgacttcga cggcatcgca cggcttggtg ctgcgctgca
     1981 tgagcgccac ggcaagctcg acattctcgt cggcaatgcc ggggtcgccg gcccctcctc
     2041 gccgctcggg catatcgagc tgaagccgtg gagcgacgtg atggcgatca acgtgtcagc
     2101 gaattttcag ttgatccgct gcatggaacc gttgctgaaa aagtccgacg ccggccgcgc
     2161 ggtgttcgtc acctcggggg ccgcaagcaa ggccagcgcc tatctcggtc cgtatgcagc
     2221 ctcgaaagcc gcgctcgaaa cgctggtgcg ggtgtgggcc cgggaaaccg cagcgacggc
     2281 tgtgcgcgtc aacctgttcg atcccggccc gatccggacg cgcatgcgcg ccaccgtgtt
     2341 ccccggcgaa gacccgttga cgctcgatac cccggaccag gccgccgaat tcatcgtgcc
     2401 gatgtgcgcg cctgactgga ccgaaaccgg caagttctat gattacaaga cgcgcacgct
     2461 gatgagtttt cgcggaccgg cgtagcgtca cgatgggcac tgcggtttcc gaatattcag
     2521 atgtcatccc cgcgaaagcg gggaaccagt aaacaccaac gccggtgatt actgggtcgc
     2581 ccgctttcgc gggcgatgac cgctatttgt ggagccgtcg ctgcgcccgc cagcacatgc
     2641 ccgccccccg aattgactcc cgaacctcgc cgctattcac tgctcgccaa gcgggaaaac
     2701 cgcagcaaag aatgcgggag gatgccatg
//
LOCUS       DATNOI010000616         2581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_257030,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000616 DATNOI010000000
VERSION     DATNOI010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257030"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..987)
                     /locus_tag="VN968_24995"
     CDS             complement(1..987)
                     /locus_tag="VN968_24995"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32523.1"
                     /translation="MKRREFITLLGGAAAAWPLAARAQQGERMRRIGVLVGLAEDDPE
                     AKARLAGFWQAFELLGWFEGRNVLVDYRYAPAGAGAQVLAKELIALQPDVILTQGTPN
                     SAALKRETSTVPVVFVGNADPISSGFVESLVRPGGNLTGFLLYEESITGKWLAMLKEM
                     APAVTRVALVSNPKTIPYDYFLRGAEAAAPSVAVQIVPSRVENAAEIERAIESFARVP
                     DGGMVLPPDTTTVVNRDLIIALAARHRLPAVYSLRIFAVAGGLMSYGTDFVNLFRQAA
                     SYVDRILRGAKPADLPVQQPTKFETVVNLKTAKALGLTVPPGLLVAADEVIE"
     gene            complement(1241..2245)
                     /locus_tag="VN968_25000"
     CDS             complement(1241..2245)
                     /locus_tag="VN968_25000"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32524.1"
                     /translation="MQFDRLKRRKFITLLGGAAAWPLAARAQQAERVRRIGVLTAYSE
                     SDPEAQARVGAFREGLQKLGWTEGRNIRIDARWATSDSALMQRFAEELVALQPELILS
                     SSRPTTAALLHQTRTIPIIFAIVADPVGSGFVASFPRPGGNVTGFTDIQPTIAGKWLE
                     LLKEIAPRVARVAFLFNPVTAPHFEYYLNPFKAAAASFAVEAIAAPVRDRSDLNSVIA
                     AQARAPNGGLIVMPDAFIVTHRAEITLLAARYRLPAVYSYRIFTDAGGLLSYGSDLVD
                     NFRRAATYADRILKEAKPSELPVQAPVKFELVINLKTATALGLDVPWFLQQRADEVIE
                     "
BASE COUNT          504 a          790 c          821 g          466 t
ORIGIN      
        1 tcattcgatc acctcgtcgg ccgccacaag caagccgggc ggcacggtca ggccgagcgc
       61 cttcgctgtt ttgagattga cgaccgtttc gaacttggtc ggctgctgga caggaaggtc
      121 ggcgggcttg gcgccgcgca ggatgcggtc gacgtaggac gccgcctgtc gaaacaaatt
      181 tacgaagtcg gttccgtagg acatgagacc accggccacg gcgaaaatac ggagcgagta
      241 aaccgcaggc aaacgatgcc gggctgcaag cgcgatgatg agatcgcgat tcacgacggt
      301 tgtggtatcg ggcggtaaga ccatgccgcc gtccggtaca cgcgcgaacg actctatggc
      361 gcgttcgatc tcggcggcgt tctccacacg actcggcaca atttggacag caaccgatgg
      421 tgccgcggct tcggcgccgc gcaggaagta atcatatggg atggtcttgg ggttagagac
      481 gagggcgact cgtgtgacgg cgggcgccat ctctttgagc atcgccagcc acttgccggt
      541 gatgctttcc tcgtacaaca ggaaaccggt gagattgccg cccggccgca ccaagctttc
      601 gacaaaacct gagctgatcg gatctgcgtt gccgacgaat acaactggaa ctgtactggt
      661 ctcccgcttc agcgcggcgc tgttcggcgt accctgggta aggatcacgt cgggctgcag
      721 cgcgatcagc tctttcgcta gcacctgcgc ccccgcacca gcaggtgcat agcgatagtc
      781 gacaagcaca ttgcggcctt cgaaccagcc aagcagttcg aacgcctgcc aaaatcccgc
      841 aaggcgcgcc ttcgcttcag gatcatcctc ggcgagaccg accagcacac cgatccgtcg
      901 catccgctcg ccctgttgcg cccgtgccgc gagtggccac gcggctgccg cgccgccgag
      961 cagcgtgatg aactcgcggc gtttcattcg gcaacctcgc tggcaggctg tgggcgatgt
     1021 cagggcctac cgaaggatag cacgtctcgc tgccgcagga gtgctgcact gcgggatttc
     1081 aacccggctt acgacggctt cgggtcatct tcgacccacg cggtcgaagc gacacgtctc
     1141 tgtttgtccg ctttgctccg aaagcgaccg tcgcaaacca aaatgtgatc cgccgctatg
     1201 tgccagaagc ggctgttagc aatcgcagca aggcagcccc ttattcgatc acctcatcgg
     1261 cgcgctgctg aagaaaccaa ggcacgtcga ggccgagcgc cgtggcggtc ttgaggttga
     1321 tcaccaactc gaacttgacc ggggcctgga ccggaagctc gctcggcttc gcctccttga
     1381 ggatgcgatc ggcataggtc gccgcgcgcc gaaaattatc gaccaggtca cttccgtagg
     1441 acagcaggcc gccggcatca gtgaatatcc ggtacgaata gacggcaggg agacggtagc
     1501 gcgccgccag caaggtaatc tccgcgcgat gagtgactat gaaggcatcc ggcatcacga
     1561 tcaggccgcc attcggcgcg cgtgcctgtg cggcaatgac ggagttgagg tcggacctgt
     1621 cgcgaacagg tgcggcgatc gcctccactg cgaacgacgc agcggcggct ttgaaggggt
     1681 tcagataata ttcgaaatgt ggcgccgtta ccgggttgaa caggaaggcg acccgggcga
     1741 cgcgcggcgc gatctccttg agcagctcca gccacttgcc cgcgatcgtc ggctgaatat
     1801 cggtgaaacc ggtgacgttg ccgcccggcc gcggaaagct cgcgacgaag ccgctgccga
     1861 ccggatcggc aacgatggcg aaaatgatgg ggatggtgcg cgtttgatgc agcagcgccg
     1921 cggtggtggg tctgcttgac gagagaatga gttcgggctg cagcgcgacg agttcctccg
     1981 cgaatcgttg catcaacgcc gagtcggacg tcgcccagcg cgcgtcgatc cggatgttgc
     2041 gaccctccgt ccacccgagc ttctggagcc cctcgcggaa cgccccgacg cgggcctgtg
     2101 cttccgggtc gctctcggaa taggcggtca atacgccgat ccgccgcaca cgctccgcct
     2161 gctgcgcgcg cgcagccagc ggccacgctg ctgcgccgcc gagaagagtg atgaactttc
     2221 gtctcttcag ccgatcgaat tgcatccgct gcccctagcc agagtggcag catagcggat
     2281 tggcgaggat caggtcaggg gctcactgca gtgcaagatt tcgacccggc taatgactgc
     2341 aacgggtcag gagcgtcgaa ttggtgccgt tcgtaacatt tccgctttac ccccacgagc
     2401 cgacgtagga gcggacattg ttgaaccgtc gttgagtgcc acaagctgag tgtatagagg
     2461 ggacctactc gatcagctcg tccgcgcgca gcaggaacga ctcgggaatg gcgagtgcga
     2521 gtgccggggc ggtagtcagg ttaataacca agtcccactt tgtgggttgc tccaccggca
     2581 a
//
LOCUS       DATNOI010000617         4637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_438349,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000617 DATNOI010000000
VERSION     DATNOI010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438349"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VN968_25005"
     CDS             complement(<1..317)
                     /locus_tag="VN968_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HXL32525.1"
                     /translation="MIRATQVRGQHRWTETPADTIVLDFDDRHRRRMAMTATRGLEFL
                     LDLENAVALRGGDALVLDDGRLIEVVAAPEPLLEIRGTDPHHLVRVAWHLGNRHLPTQ
                     IMA"
     gene            complement(328..627)
                     /locus_tag="VN968_25010"
     CDS             complement(328..627)
                     /locus_tag="VN968_25010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HXL32526.1"
                     /translation="MIYVVATSQVKPELRDAFIKGAKECIAATRKEKGCIAYDSHTSI
                     NDPNLFVVVERWESREDLNAHSRAPHMKVWREYSAPLKVSPTVIEIISDGKVEKL"
     gene            complement(685..984)
                     /locus_tag="VN968_25015"
     CDS             complement(685..984)
                     /locus_tag="VN968_25015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HXL32527.1"
                     /translation="MIYVVATLTVKPETRAEFIAGATACIRETRKEAGNIAYDLHESV
                     TDPTKMVFVEQWENAEALVPHRAAEHMKTFGRIAVKCMSAPPKIEIITPEKVDVR"
     gene            complement(1031..2743)
                     /gene="ureC"
                     /locus_tag="VN968_25020"
     CDS             complement(1031..2743)
                     /gene="ureC"
                     /locus_tag="VN968_25020"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HXL32528.1"
                     /translation="MSVKIKRSVYADMFGPTTGDRVRLADTDLIIEVEKDFTVYGEEV
                     KFGGGKVIRDGMGQSQVTNKQGAADTVVTNALIVDHWGIVKADVAIKEGYISAIGKAG
                     NPDIQPGVTIVIGPGTDVIAGEGKILTAGGFDSHIHFICPQQIEHALMSGVTSMLGGG
                     TGPSHGTFATTCTPGPWHMARMIQSFDAFPVNLGISGKGNASRPAALVEMINAGACAL
                     KLHEDWGTTPAAINNCLNVADDHDVQVMIHSDTLNESGFVEDTVKAFKGRTIHAFHTE
                     GAGGGHAPDIIKIAGLKNVLPSSTNPTRPFTRNTIDEHLDMLMVCHHLDPSIAEDLAF
                     AESRIRKETIAAEDILHDLGALSMMSSDSQAMGRLGEVIIRTWQTADKMKKQRGALPQ
                     DKGNNDNFRVKRYIAKYTINPAIAHGVSKLIGSVEKGKLADLVLWSPAFFGVKPECII
                     KGGSIVAAPMGDPNASIPTPQPVHYQPMFAAYGKSLTASSVVFSSKAAVSSGLAKKLG
                     IDKKLYPVENTRGGISKKSMIHNGATPKIEVDPETYEVRADGELLTCAPAEVLPMAQR
                     YFMF"
     gene            complement(2835..3140)
                     /locus_tag="VN968_25025"
     CDS             complement(2835..3140)
                     /locus_tag="VN968_25025"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HXL32529.1"
                     /translation="MIPGEFFIQDGEIELNAGRKTVTLTVANSGDRPIQVGSHYHFFE
                     TNPALKFDRRRARGMRLDIAAGTAVRFEPGQTRDVQLVALAGKRVVYGFRGDVMGKL"
     gene            complement(3272..3574)
                     /locus_tag="VN968_25030"
     CDS             complement(3272..3574)
                     /locus_tag="VN968_25030"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HXL32530.1"
                     /translation="MNLSPREKDKLLISMAAMVARRRLERGVKLNHPEAIAIISDFIV
                     EGARDGRTVADLMQAGAQVITRAQCMDGIAEMIHDIQVEATFPDGTKLVTVHEPIR"
     gene            complement(3601..4437)
                     /locus_tag="VN968_25035"
     CDS             complement(3601..4437)
                     /locus_tag="VN968_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HXL32531.1"
                     /translation="MRSDTTSAASAIFAANRAQGSVTFDVHQVEGVSRRRQLHESGSL
                     RVRFPSPEAEGLSAVFVNTAGGVAGGDRFDIDISTGEGSRLAVTTAAAEKIYRAQGPA
                     AQVNIALKAAAGAHLAWLPQETILFDRARVLRRIDIDLAESASLLLCEIVVFGRSAMG
                     ERMLHGEFVDRWRMRRGGRLVFAETVRLDGDIGEKLARPAIAKGGVAIGTALIVPGDE
                     ALVERIRELATSSGGEVGISCWNGFAMARFCAQDAARLRADMMAVLGVASGSALPRLW
                     LN"
     gene            complement(4491..>4637)
                     /locus_tag="VN968_25040"
     CDS             complement(4491..>4637)
                     /locus_tag="VN968_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32532.1"
                     /translation="IAIVLVEQYLDFACELGDNFAVMDRGAVKYACDRSNLDPGEISR
                     QMAL"
BASE COUNT          801 a         1451 c         1455 g          930 t
ORIGIN      
        1 ttggccatga tctgcgtcgg cagatggcgg ttgccgagat gccaggcgac tcgaaccaga
       61 tggtgcgggt cggtgcccct tatctcgagc aacggctccg gcgccgccac cacttcgatc
      121 aaacgtccat cgtcgagcac cagcgcgtcg ccgccgcgca gcgcgaccgc gttttccaga
      181 tcgagcagaa actcaaggcc ccgcgtggcg gtcatcgcca tccgccgccg gtgacggtcg
      241 tcgaagtcga gcacgatggt atcggccggc gtctccgtcc agcgatgttg cccgcggacc
      301 tgggttgcgc ggatcatgcg agaacactca cagcttctcg accttgccgt cgctgatgat
      361 ctcgatcacg gtcggcgata ccttcagggg cgcggagtat tcacgccaga ccttcatgtg
      421 cggcgcccgg ctatgcgcat tgaggtcctc gcggctttcc cagcgctcga ccaccacgaa
      481 aaggttggga tcgttgatgc tggtgtggct gtcataggcg atgcagcctt tttccttgcg
      541 ggtggccgcg atgcattcct tggcgccctt gatgaaggcg tcccgcaatt ccggcttcac
      601 ttgcgaggta gcgacgacgt agatcatgga tcggtctccc ggttcttgtt gtttatcgca
      661 agtcttgttt gtcgcaagtc ttgcttatcg aacgtcgacc ttttccggcg tgatgatctc
      721 gatcttcggc ggcgccgaca tgcatttcac ggcgatgcgg ccgaacgtct tcatgtgctc
      781 ggcggcgcga tgcggaacca gcgcctcggc gttttcccat tgctcgacga acaccatctt
      841 ggtcggatcg gtgacgcttt catgcaggtc ataggcgata ttgccggcct ccttgcgggt
      901 ttccctgatg caggcggtcg cacccgcaat gaattcggcg cgcgtttcag gcttcacggt
      961 cagcgtggca acaacgtaga tcacgaaatc ctcccggtct ttgttcaggc tttgggtgcc
     1021 gggagcaagc ttagaacatg aaatagcgct gcgccatggg cagcacctcg gccggcgcgc
     1081 aggtcagcaa ttcgccgtcg gcgcgcacct cataggtttc cggatcgacc tcgatcttgg
     1141 gcgtggcgcc gttgtggatc atgctcttct tggagatgcc gccacgggta ttttcgaccg
     1201 ggtagagctt cttgtcgatg ccgagcttct tggccagccc cgaggacacc gccgccttcg
     1261 aggaaaacac cacggaggac gccgtcagcg atttgccgta agccgcgaac atcggctgat
     1321 aatgcacggg ctgcggcgtg ggaatggacg cgttggggtc gcccatcggc gctgcgacga
     1381 tggagccgcc cttgatgatg cattcgggtt tgacgccgaa gaaggccggc gaccacagca
     1441 ccagatcggc gagcttgccc ttttccaccg agccgatcag cttcgagacg ccatgggcga
     1501 tcgcggggtt gatggtatat ttcgcgatgt agcgcttgac ccggaaatta tcgttgttgc
     1561 ctttgtcttg cggcaacgcg ccgcgctgct tcttcatctt gtccgcggtc tgccaggtgc
     1621 ggatgatgac ctcgccgagc cggcccatgg cttgcgaatc cgaggacatc atcgacagcg
     1681 cgccgaggtc gtgcaggatg tcttccgccg cgatggtttc cttgcgaatg cggctttcgg
     1741 cgaacgcaag gtcctcggcg atcgagggat cgagatggtg gcacaccatc agcatgtcca
     1801 gatgctcgtc gatggtgttg cgggtgaacg gccgcgtcgg attggtcgag gacggcagca
     1861 cgtttttcaa gccggcgatc ttgataatgt cgggtgcgtg accgccaccg gcgccttcgg
     1921 tatggaaggc gtgaatggtg cggcccttga acgccttgac ggtatcttcg acgaagccgg
     1981 actcgttcag cgtgtcggaa tggatcatca cctggacgtc gtggtcgtca gcgacgttga
     2041 ggcagttgtt gatggcggcc ggcgtcgtgc cccagtcctc atgcagcttc agcgcacagg
     2101 cgccggcgtt gatcatttcc accagcgccg ccgggcgcga cgcgttgccc ttgccggaaa
     2161 tgccgaggtt gaccggaaac gcatcgaacg actggatcat gcgcgccatg tgccagggac
     2221 ccggcgtgca ggtggtcgca aaggtgccgt gtgacggacc ggtgccgccg ccgagcatcg
     2281 aggtgacgcc ggacatcagc gcgtgctcga tctgctgcgg gcagatgaaa tggatgtggc
     2341 tgtcgaaccc gcccgcggtg aggatcttcc cctcgcccgc gatgacgtcg gtgccgggac
     2401 cgatgacgat ggtgacgccg ggctggatgt cgggattgcc ggccttgccg atggcgctga
     2461 tatagccttc cttgatggcg acgtcggcct tcacgatccc ccagtggtcg acgatcagcg
     2521 cattggtgac gacggtatcg gccgcgccct gcttgttggt gacctgggat tgccccatgc
     2581 cgtcgcggat caccttgccg ccgccgaatt tcacctcctc gccatagacc gtgaaatcct
     2641 tttcgacctc gatgatcaaa tccgtgtcgg ccagccgcac ccgatcgccg gtggtcggtc
     2701 cgaacatgtc ggcatagacg gaacgtttga tttttacgga catcatctcg ctccatcagc
     2761 ggaagtgctc cctcccccct tgcgggggag ggttagggag gggggtgcag cggcagccat
     2821 cgtatcgcga gaaatcacag cttccccatc acgtcgccac gaaatccgta gacgacgcgc
     2881 ttgccggcga gtgccacgag ttgcacgtcg cgcgtttgcc cgggctcgaa gcggaccgcg
     2941 gtgccggcgg cgatatcgag ccgcatgccg cgggcgcgtc tgcggtcgaa tttcagcgcc
     3001 ggattggtct caaagaaatg atagtgcgaa ccgacctgga tcggccggtc gcctgagttg
     3061 gcaaccgtca gcgtcaccgt cttgcggccg gcgttgagtt cgatctcgcc gtcctggatg
     3121 aagaactcgc cggggatcat ttctcctcct cgtcattccg gggcgcgtcg aagacgcgaa
     3181 ctatgatgtg caattgcaca tctgagaatc tcgaaattaa taacctctgg attccgggtt
     3241 cggccgttct ggccgccccg gaatgacgtt gtcatctgat cggctcgtgc acggtgacga
     3301 gttttgttcc gtcgggaaac gtcgcttcga cctggatgtc atggatcatt tcggcgatcc
     3361 catccatgca ctgggcgcgg gtgatcacct gggcgccggc ctgcatcagg tcggccacgg
     3421 tgcggccgtc gcgcgcgccc tcgacgatga aatcggaaat gatcgcgatg gcttccggat
     3481 ggttgagctt gacgccgcgc tccagccgcc gtcgcgccac catcgccgcc atcgagatca
     3541 gaagcttgtc cttttcgcgg ggagacagat tcatgcgagc actctgataa ttacgaccat
     3601 ctaattgagc cacagccgcg gcagcgccga gccagaggca acgccgagca cagccatcat
     3661 gtcggcgcga agcctcgcag catcttgggc acagaagcgc gccattgcaa acccattcca
     3721 gcaggagatg ccgacctcgc cgccggacga tgttgccagt tcgcgaattc tttccaccaa
     3781 tgcctcgtca cccggcacga tcagggcggt gccaatggcc acgccgcctt ttgcgatcgc
     3841 cggcctggcg agcttttcgc cgatgtcgcc atcgagccgc acggtttccg cgaacaccag
     3901 ccggccgccg cgccgcattc gccagcgatc gacgaactcg ccatgcagca tgcgctcgcc
     3961 catcgcggag cggccaaaca ccacgatttc gcacagcaac agcgaagcgc tttccgcaag
     4021 atcgatgtcg atccggcgca gcacccgtgc ccggtcgaac agaatggtct cctgcggcag
     4081 ccaggcgaga tgcgcgcctg ccgcggcctt caaggcaatg ttgacctgcg cagccggccc
     4141 ctgggcgcga tagatttttt cagccgcggc cgtcgtcact gcgagacgcg atccctcgcc
     4201 ggtcgagatg tcgatgtcga aacgatcgcc gccggcgacg ccgcccgcgg tgttgacaaa
     4261 aaccgccgag aggccttccg cctcgggcga gggaaagcgg acgcgcagcg agccggattc
     4321 atgcagctgg cggcggcggc tgacgccctc gacctggtgc acgtcaaacg tcaccgaacc
     4381 ctgggcccgg ttggccgcga aaatcgccga ggccgcactg gtggtgtcgc tccgcatcgg
     4441 cccccaaatc ttcccaaaaa gggcgcgcga cctctgccgg tcgcctttgc ctagagcgcc
     4501 atctgacgac tgatttcgcc gggatcgaga ttgctgcgat cgcaggcata tttcacggcg
     4561 ccccggtcca tgaccgcgaa attgtcgccg agctcgcagg caaagtcgag atattgttcg
     4621 accagcacga tcgcgat
//
LOCUS       DATNOI010000618         5820 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2033503,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000618 DATNOI010000000
VERSION     DATNOI010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033503"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VN968_25045"
     CDS             complement(<1..695)
                     /locus_tag="VN968_25045"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXL32533.1"
                     /translation="MNLEKLTFRSVTARSVLVPLRRPIVSKVGLFDQWPMILIELVTE
                     QGIVGRSYLEPYLKHAARYIVPAIHDLAEQRKGHAIYPIEDFQNGRRALNLIGYEGVS
                     MIAVSGLDMAAWDALAKAGGMPLAVLLGGSTGPVPAYNSNGLWLTEVASLGDDARALV
                     AEGGFKALKLRLGRDRLADDLAAIEAVRTAAGADIKLMVDFNQGLSLGDALHRCHALD
                     RQGLYWFEEPIAY"
     gene            785..1465
                     /locus_tag="VN968_25050"
     CDS             785..1465
                     /locus_tag="VN968_25050"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL32534.1"
                     /translation="MDAVVNSLTGIHSGNDGGEMPRSSAPTRQAILDAAYGLFRRKGF
                     VRVSMDEIAASAAVTKRTLYYHFTSKDLLLAEVLEAQHALALAAFRTFGEGLAGSPES
                     IIDQLFSDLAVWSDQPRWAGSGFTRLVIELADLRGHPARLIARRHKAMLEGHLADLLA
                     NADAPSPRELAREIWLLSEGAIALILIHGDRSYAAAAAAAAKKLIRSESALRRSHSAQ
                     RILAARRK"
     gene            complement(1490..2731)
                     /locus_tag="VN968_25055"
     CDS             complement(1490..2731)
                     /locus_tag="VN968_25055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HXL32535.1"
                     /translation="MTSAAFDFAPLLPAGLPAPAARWTGLAKYSFVGGNNDPEQVPVE
                     GLIDAANAVLRREGKTLATYGLASGPQGYRPLREFLTAKLKRDAGIGCAADDILIVSG
                     SLQALDLVNATLLARGETVIVEQETYQGALNRLTRLGVNAVGVPLDQEGMRMDALAAA
                     LADLKRRGVTPKYIYTIPTVQNPTGSILPEARRAELLKLAAQYGVPIFEDDCYADLIW
                     NGQRPPALYAMGANRGVIHIGSFSKSIAPALRVGYIVAPWEMLSRMLALKTDAGSGAL
                     EQMVLAEYCAPHFTSHVPKLTRGLRAKLDTLMEALDEQFGTAAEFRDPKGGIFLWVKL
                     PDNVDTLKLYQAALAAGVAINPGPEWSTDKAYGRSRLRLCFASPSREQIREGIAVLAE
                     VCRREFGVPARIANVEKQVRA"
     gene            complement(2793..3335)
                     /locus_tag="VN968_25060"
     CDS             complement(2793..3335)
                     /locus_tag="VN968_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HXL32536.1"
                     /translation="MCHSLVGRLFCTVVLGLLAIHGPAARAQTAAGIEQTFANSLTTI
                     PTENLGVSGAFYVPAYSSVSMSQGKTRADFSVTLSIHNASETRPLVLKRIAYFETGGE
                     MVESYLKAPIALKPLSTVEVFVPTTDVRGGTGANFLVDWAATGEIAEPVVEALMLGGL
                     GSGHYAFISQGRPIKVVGKN"
     gene            3406..5133
                     /locus_tag="VN968_25065"
     CDS             3406..5133
                     /locus_tag="VN968_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXL32537.1"
                     /translation="MHELIRDITLSILFAWVLGLLAHFFRQPLILAYLIAGFLIGPFG
                     TGLVKSQESISIISELGLIFMLFMIGLEIDLKKIVRAGRVILFAAGGQLAGGCVLGVL
                     FFVGIGLPMEAGAFDALYLCIACALSSTVIIVKVLYEKRELDTLPGRVTLGVLVLQDI
                     FAILFLAVQPSLGNLQVGVILMSIGRVGVLVATAMIVSRYVLPRLFHQIARRPELILL
                     GALAWCFLIGEIAERLHLSREMGSLVAGVSLSTFPYALDVTAKVTTLRDFFITLFFVA
                     LGMTIPIPNMSVIELALLIAAFAVVSRVVTTFAPLYLMKQGLRASLLPAINLAQISEF
                     SLVVIQTGVAAGHIQTRTASAASFAFVILAVLSTFVMMRSDQITRLAIGPLKRIGIRD
                     LDHDHAAEAGHNGGHGDARRIVILGFFRAASALLSEIERQNPLLLDQISVVDFNPQVF
                     RTLSERGLHVIYGDISNVDTLLHAGVGKAEIIILSVPDSLLKGANNEKLVRHVRTLNP
                     TAKIVSTADLLSDVGDLYAAGADYVTVTRLSDAHELFTVIEAADAGLLDDKRAEIDGR
                     LSDRREVLP"
     gene            5266..>5820
                     /gene="ribB"
                     /locus_tag="VN968_25070"
     CDS             5266..>5820
                     /gene="ribB"
                     /locus_tag="VN968_25070"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HXL32538.1"
                     /translation="MADPVQEVLQAFAHGELVVVTDDDDREGEGDLIVAASLCTAEKM
                     AFIIRHTSGIVCAPITTEDARRLRIDPMVAHNESSHTTAFTVSIDYKPDGGTGISADE
                     RASCCRALANPNAGANDFARPGHVFPLIARDGGVLLRSGHTEAAVDLCKLSGLPPVGV
                     ISELMNDDGTVMKGEQVARFAAAHK"
BASE COUNT         1001 a         1839 c         1817 g         1163 t
ORIGIN      
        1 tcgtaagcaa tcggttcctc gaaccagtag aggccttgcc tgtcgagcgc atgacagcgg
       61 tgcagtgcat cgccgagact aaggccctgg ttgaaatcca ccatcagctt gatatccgcg
      121 ccggccgcgg tgcgcaccgc ctctatcgcc gcgagatcgt cagccaatct gtcgcggccc
      181 aaccgcagct tgagggcctt gaagccgcct tcggcgacaa gcgcccgcgc gtcatcgccc
      241 aaactggcta cctccgtgag ccacaggcca ttgctgttgt aggccggcac cggaccggtc
      301 gagccgccca acaagacggc gagcggcatg ccgcccgcct tcgccagggc gtcccacgcc
      361 gccatatcga gcccggatac ggcgatcatc gatacgcctt cgtagccgat caggttcagc
      421 gcccggcgcc cgttctgaaa atcctcgatc gggtagatgg cgtggccctt gcgctgctcg
      481 gcaaggtcgt ggatcgcggg cacgatgtag cgcgcggcgt gcttcagata aggttcgaga
      541 tagctgcggc cgacaatacc ttgctcggtt acgagctcga tgagaatcat cggccattga
      601 tcgaacaggc cgaccttgga gacgatcggc ctgcgaagcg gtaccaggac cgaccgggcg
      661 gtcacggacc ggaaagtgag cttctcgaga ttcaccgaat cctcccacca ccagcgcggc
      721 gcgccgttct cgccatcgtg ccgctgcgca tccgatattg ggcgaccgtt tcattcgaga
      781 cgggatggat gctgttgtaa actcactaac cggtatacat tccggaaatg atggaggaga
      841 gatgccgcga tcctcggcgc ccacccgcca ggccattctc gatgccgcct acggtctatt
      901 ccgccgcaag gggttcgtgc gtgtgagcat ggatgagatc gcggcatccg ctgccgtcac
      961 caagcggacg ctgtactatc attttacgag caaggacctg cttctcgccg aggtgctcga
     1021 agcgcagcat gctctggctc ttgcggcctt tcgcacgttt ggcgaaggtc ttgccggctc
     1081 tcccgagagc atcatcgatc aacttttcag cgatctcgcc gtctggtcgg accagccgcg
     1141 atgggccggt tcgggattta ctcgccttgt tatcgagctt gccgatctcc gtggacaccc
     1201 cgcgcgcctg atcgcgcgcc gtcacaaggc aatgctcgaa ggtcacctgg cggacctttt
     1261 ggcgaacgcc gatgctccgt caccccgcga actcgcccgc gaaatctggc tgctttccga
     1321 gggcgcaatc gccctgattc ttatccatgg cgaccgcagt tacgctgcgg cggccgcggc
     1381 agcggccaag aaactgatcc gctcggaatc tgcgctcaga agaagtcaca gcgcgcaacg
     1441 gatactcgcc gcgcgaagga aatagcatca acccgtccca aatcggcggt tacgcgcgaa
     1501 cctgcttctc gacattcgcg atccgcgccg gcacgccgaa ttccctgcgg cacacctcgg
     1561 cgagcaccgc tatgccctcg cggatttgct cgcgggacgg actggcgaag cacaaccgca
     1621 accggctgcg gccataggcc ttgtcggttg accattccgg tcccgggttg atcgcgacgc
     1681 cggcggcaag tgcggcttga taaagtttca gcgtatcgac gttgtccggc agcttcaccc
     1741 acaggaagat gccgcctttg ggatcgcgaa actccgcggc ggtgccgaat tgctcgtcaa
     1801 gcgcttccat cagcgtgtcg agcttggcgc gcaggccgcg tgtcagcttc ggcacatgtg
     1861 aagtgaaatg cggcgcgcaa tattccgcga gcaccatctg ctcgagggcg ccggagccgg
     1921 cgtcggtctt cagtgccagc attcgcgaca gcatctccca gggtgctacg atgtagccga
     1981 cccgcagcgc cggcgcgatc gattttgaga acgagccgat gtgaatgacg ccgcggttcg
     2041 cccccatcgc atagagcgcg ggcgggcgct ggccgttcca gatcagatcg gcatagcaat
     2101 cgtcctcgaa gatcggcacg ccatattgcg cagcgagctt caggagttcg gcgcggcgcg
     2161 cctccggcag gatgctgccc gtcgggttct gcacggtcgg gatggtgtag atgtatttcg
     2221 gcgtgacgcc gcggcgcttg aggtcggcga gcgcggccgc cagcgcgtcc atccgcattc
     2281 cctcctggtc gagcgggacg ccgaccgcgt tgacgccgag ccgggtcagc cggttcagtg
     2341 caccctgata ggtctcctgc tcgacaataa cggtttcgcc gcgcgccagc agtgtcgcgt
     2401 tgacaaggtc gagcgcctgc agcgaaccgg agacgatcag gatgtcgtcg gcggcgcagc
     2461 cgatgccggc gtcccgcttg agctttgccg tcagaaattc gcgaagcgga cggtagcctt
     2521 gcggaccgct ggcaagccca taggtggcga gggttttgcc ttcgcgcctg agaaccgcgt
     2581 ttgcagcgtc gattaggcct tcgaccggga cctgctcggg atcgttgttg ccgccgacaa
     2641 aactgtattt cgcaagcccc gtccaccttg ccgccggcgc gggcagtccc gccgggagca
     2701 gtggcgcgaa gtcgaatgct gctgatgtca tgaaggtttc ctctgcttgt tcttctcgtt
     2761 gagagagcca gtatcgcggc tctccggcag tttcagtttt tgccgacgac cttgatcggc
     2821 cggccctggc tgatgaaagc atagtgtccg ctgccaagcc cgccgagcat cagcgcctcg
     2881 accacgggtt cggcgatctc gccggtggct gcccagtcga ccaggaagtt agcgccggtt
     2941 ccgccccgca cgtccgtggt cgggacaaag acttcgacgg tcgacaaggg ttttagggcg
     3001 atcggcgcct tgagatagct ctccaccatc tcgccgccgg tttcgaaata ggcaattcgt
     3061 ttcaagacca gcggccgcgt ctcggaagcg ttatggatgc tcagcgtcac cgagaaatca
     3121 gcgcgtgtct tgccttggct catcgagacg ctcgaatagg ccggaacgta gaatgcgccg
     3181 gacacaccga gattttccgt tggaatggtg gtcagcgaat tggcgaaggt ctgctcgatg
     3241 ccggcggccg tttgggccct cgccgcgggc ccatggatgg ccagcaggcc cagcacgacg
     3301 gtgcaaaaca agcgcccgac aaggctgtga cacattgctt gtttgacctt ccctgtgatg
     3361 tccttaggtt ggcgaatcga agataccgcc ggccggcctt gagacatgca tgaacttatt
     3421 cgcgacatca ctctatccat cctgttcgca tgggtgctgg ggcttttagc tcatttcttc
     3481 cggcagccgc tgatcctcgc ctaccttatc gccgggttcc tgattggtcc attcggcacc
     3541 ggcttggtca agtctcagga atccatcagc atcatctcgg agcttggcct gatcttcatg
     3601 ttgttcatga tcgggctgga aatcgatctg aagaagatcg tgcgcgccgg ccgggtcatc
     3661 ctgtttgccg ccggcgggca gctggcgggc ggctgcgtgc tcggcgtcct gttcttcgtg
     3721 ggcattggtc tgccgatgga ggcaggcgct ttcgatgcgc tgtatctctg catcgcctgc
     3781 gcgctgagca gcaccgtcat catcgtcaag gtgctgtacg aaaaacgcga gctcgatacg
     3841 ctgcccggcc gcgtcaccct cggtgtgctg gtgctgcagg atatcttcgc aatcctgttt
     3901 ctggcggtgc agcccagcct cggcaatctc caggtcggcg tgatcctgat gtcgatcggc
     3961 cgcgtcggcg tgctggtggc aaccgcgatg atagtgagcc ggtacgtttt gccgcggctg
     4021 tttcatcaga ttgcgcgccg cccggaactg atcctgctcg gcgcgctggc gtggtgcttt
     4081 ctgatcggcg agatcgccga gaggctgcat ctgtcacgtg aaatgggatc gctggtggcc
     4141 ggcgtgtcgc tgtcgacatt tccctatgca cttgacgtca ccgccaaggt cacgacgctg
     4201 cgcgatttct tcatcacgct gttcttcgtc gcattgggca tgacgatccc gatcccgaac
     4261 atgtcggtga tcgagctcgc gctgctgatc gcggcattcg cggtggtcag ccgcgtggtg
     4321 acgacgttcg cgcctcttta tctgatgaag cagggcctgc gcgccagcct gctgccggcg
     4381 atcaatttgg cgcagatcag cgagttttcg ctggtggtga tccagaccgg cgttgcggcg
     4441 ggtcatatcc agacccggac cgcgagtgcg gcttctttcg cattcgtgat tctggcggtg
     4501 ctgagcacgt tcgtgatgat gcgcagcgac cagatcacgc ggcttgcgat cggtccgctg
     4561 aagcggatcg ggatccgcga cctggatcac gatcatgcgg ccgaagcagg ccacaacggc
     4621 ggccacggcg acgcacgccg gatcgttatc ctgggatttt tccgggcggc gagcgcgtta
     4681 ctgagcgaga tcgaacggca gaacccgttg ttgctcgatc agatcagcgt cgtcgatttc
     4741 aacccgcaag tgttccggac gttgtccgag cgcggcctgc acgtgattta cggcgatatc
     4801 agcaatgtgg atacgctgct gcatgccggc gtcggcaagg cggagatcat tatcttgagt
     4861 gtccccgatt ccttgctgaa aggcgccaac aacgaaaagc tggtccgcca tgtgcgtacc
     4921 ctcaatccga ccgcaaagat cgtctccacc gccgacctct tgtccgacgt cggcgatctt
     4981 tatgcggccg gcgccgacta tgtcacggtg acccgtctca gcgacgccca tgagctattc
     5041 acggtgatcg aggccgccga cgctgggctg ctggatgaca agcgcgccga gatcgacggg
     5101 cgattgagcg atcgccgcga agtgctgcca taggctttga gtctggtttc ggcgtcagga
     5161 cgcggcaagc cgatgtctcg ccagcgacgt tctgccctgc gtattggcgc tggcgcgggc
     5221 gcgtgagtcc ggctattgct ccctgcacag gagtgagacc ctagaatggc cgatccagtc
     5281 caggaagttc tgcaagcctt cgctcacggc gagctcgtcg tcgtcaccga tgacgacgat
     5341 cgcgaaggcg agggcgatct gatcgtggcg gcctcgctct gcaccgccga aaagatggcg
     5401 tttatcatcc gccacacctc cggcattgtc tgcgcgccga ttaccaccga agacgcgcgc
     5461 cggctgcgga tcgatccgat ggtcgcgcac aatgaatcca gccacaccac cgcctttacg
     5521 gtgtcgatcg actacaagcc cgacggtggc accggcattt ccgcggacga acgcgcttcc
     5581 tgctgccgcg cgctggccaa cccgaatgcc ggcgccaacg attttgcccg tcccggccac
     5641 gtctttccgc tgatcgcgcg cgacggcggg gtgctgctcc gctcgggcca caccgaggcc
     5701 gcggtcgatc tctgcaagct gagcgggctg ccgccggtcg gggtcatcag cgagttgatg
     5761 aacgacgacg gcaccgtcat gaagggcgag caggtcgcgc ggttcgccgc cgcccacaag
//
LOCUS       DATNOI010000619        18166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2033758,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000619 DATNOI010000000
VERSION     DATNOI010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 18166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18166
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033758"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..3745)
                     /locus_tag="VN968_25075"
     CDS             complement(50..3745)
                     /locus_tag="VN968_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HXL32539.1"
                     /translation="MPAPKRSLPVGGRGFGGDQSYNQRPQREAMARNTSPKGSKPRAD
                     VRLSQWDDAGWDQDHDDAAGHRARRLLSNPASGIHRAGDYAGALRRWLATERWVKRIA
                     IVVAVLMVIFAGCFGGLWWRLGAGPINLEMATPWLAAAIEENIGHGNTVEVGGTQIER
                     AGRIRIAVRIRDIIVRDRDHVIVASAPKAEVRLSGTALLMGQLRAESLNLVDAELAVR
                     ITPDGYVTVSAGDTAKPLATGVTSKKQAGIPATFPRQIPAAPPGTAAPSSPAPAAGPD
                     TPSGLLAGLDWLDSLSLTGLDGQNLNEIGLKNGNLIVDDQQRGNKWNFENITLSLRRP
                     SGGGVALSVGEEGGKAWSLRVVVGPPANGVRSVDIRANKVSTTNILLALRMKDLTYTA
                     DLPLTGELKGELGRDGLPTYFTGKLIAGEGHIIDTDTPDYPMAIDSAEISVEWDSGRR
                     VLVAPFKIISGANRVTLLAHLEPPNDSVTDWQLGFSGGTIVLAGDNGEQPLIFNRIAI
                     GMRFDTDKKRVLLTQADISNGEIGFAGTGNIDYSGEARLQLGFAGTPMSAAALKRMWP
                     ILIVPEVREWVIERIERGSLQRIEIGVNSPVRNLSRRGPPIPDDGLAVNIVASGVTLR
                     PVDTLPSVRDADLKAHVTGRTATVTIGQGAVDTPAGRKLNITDFVFEVPDMAPKPSPA
                     RIKFRIDGPVPAVAEILASDRLSEFSGTLIDPNSSKGTVAALVTLGLPVKRELTKADT
                     SYTVTADVGGFGADRLVMNQKLEADKLKVVANNGGYQVKGDVKINGQPALLDYRKPND
                     GDADIKLQATLDDASRARLGMDLGPAVTGNLPIKLIGKIGGPDRDSRMGIEADLTALR
                     LDNVLPGWVKLPGKSSRAVFNVVQKPQSTRFEDIVIDGGGVSIKGSLEVDQNGDLISV
                     NFPTYSPSEGDKTSLKAERGSDGVLKVTMRGDVFDGRGFLKSAMSGKEADPKSKTRNL
                     DFDVDVKLGAVAGHNGEAVRSLDVKLSRRNGTIRSFALNGKLGRDTPLTGNFGRPAQG
                     REVIYLETNDAGAFFRFTDTYSKVVGGQLQLTMDPPTAEPKTRDGLINIRNFSVKGEA
                     SLDRIAAGGSTGVQNGVAFSALRAEFTWQNGQLTIRDGVVKGPMVGGTIGGNIDFPAN
                     QVRMSGTFVPMYALNNMLGQIPIVGLFMGGSNEGLIGVTYEVVGTTDKPTLRVNPISA
                     IFPGVSRKIWEFNTGKPPAEFPAPNNN"
     gene            3946..4737
                     /locus_tag="VN968_25080"
     CDS             3946..4737
                     /locus_tag="VN968_25080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXL32540.1"
                     /translation="MSKKTPKNSSKPSSGKTTRVQAKISGKTPLKTRNATAKKSGKRA
                     DKQSGSAAARASHKAPSKKSKSPRAGESTLRAAAESKVAGAKPKAKSPAASKPSGRRP
                     DASAGLKSELAEGTKAPAFRLPRDGGETVSLADYSGKKLVLFFYPRADTPGCTREAID
                     FTRLADAFAESQTAIVGVSADPLRAQEAFRDKHQLSVPLVSDEAHEMLESYGVWGEKS
                     MYGRTFQGILRTTVLIGADGRIARIWRNVKVDGHADDVLAAARAL"
     gene            4839..6218
                     /locus_tag="VN968_25085"
     CDS             4839..6218
                     /locus_tag="VN968_25085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXL32541.1"
                     /translation="MSNRSGHCSEHTQHHHQHDHAKAALRRPATSQASVPSRSQRAAE
                     AGYTLVHAGKQVRFGPVVFWIVVGTVTMLGMWSAATATYFAFRDDVLTRLIARQAEMQ
                     YAYEDRIAELRAKVDRSTSRQMLDQEQFDQKLDQIMRRQTALESRATALGAIPDASVT
                     GSIRAPSRGAAATDAAASSGTPKPSPISDTVIFVAPPDREARLESRAPVIVNPRSSQF
                     AKVQGVDSVLARLQGSLDQIEGRQIAALSSVEESMESRVRRMRGVFTDLGLNMAQLEA
                     ATPRAGVGGPFVPVKLPANAGAFERQLYRINTTRAQVERLNRTLALVPYRKPVVGEVE
                     FTSGFGVRSDPFLGRPAMHTGLDFRAAMGDPVRATANGKVVSSGWAGGYGRMIEIDHG
                     NGLSTRYGHLSEIGVKVGDQIRIGQVIGAVGSTGRSTGPHLHYETRIDGDAVDPQKFL
                     RAGVRLSAG"
     gene            6270..6641
                     /locus_tag="VN968_25090"
     CDS             6270..6641
                     /locus_tag="VN968_25090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32542.1"
                     /translation="MTAFKSLMLAGVALAISAGVAPRAYAQTGSTELPPDVTAFVSRR
                     ASCSEWSKKAIDPGWTAKIEAIYTNLQSLKCFDIMNDERALRQKYAGNPEIRASLGAG
                     VITKFVTRLPVRIAVPPALDR"
     gene            complement(6638..7864)
                     /locus_tag="VN968_25095"
     CDS             complement(6638..7864)
                     /locus_tag="VN968_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32543.1"
                     /translation="MPIWMRSLVALAGFAAAAALTATAASAEKKYDPGASDTEIKIGN
                     IMPYSGPASSYGVIGKTEAAYFNMINAEGGINGRKINFISYDDAYSPPKAIEQARKLV
                     EGDEVLLLFQPLGTPSNSAIQKYMNAKKVPQLFVASGATKWGDPKNFPWTMGFQPNYQ
                     SEGRIYARYILDHYPNGKIAVFWQNDDAGKDQVKGLRDGLGEKVSMIIADKSYEVSDP
                     TIDSQIVALRDSGADIFFSWAAPKGSAQAIRKVGELGWKPKFFLANTATSVAAVLKPA
                     GLQYSKDIISTAYLKDPTDLAWKEDPGVKTWQAFMDKYYPDGDKTNANNTYGYVQAQA
                     MVQVLKQCGDDLTRENVMKQAANLKDFSTDMMLPGIKVNTSADDFFPIEQMQLMKFNG
                     ETWELFGDVIDGEVGH"
     gene            8072..10180
                     /locus_tag="VN968_25100"
     CDS             8072..10180
                     /locus_tag="VN968_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HXL32544.1"
                     /translation="MGFHAARWLGARLLGAAGFHQLGSDFANARIACVREKLGRGETV
                     YLAGIGPPGTHNSGVALVEVSQANGPRLIVNNEEERFSGNKHTTEYPVASIDAMVATL
                     RGIGRDIGDIDAWLTSWNYPDLAGTLVRSVLEELPQSVRLLRTTDAAGFDGRRLDQMT
                     RTPKILGRQLGLPERVPLICMPHHDNHAWFSFAASPFACDHDPVAIAVLDGTGDLGSI
                     SLYVAEAGVMRRLYCNDSVFDSLGAFYSVVSSTQGGWTWLSSEGRYMGAAAWGDMNRA
                     SNPYYARLKDVLHFGSNGEVRLNRAVANWYCDPFDHPYKPALIEILGPPLRPDQLWNP
                     DAVLRVEDINHRPDTKDRLDKAAATQLVFEDAMIHVVDHLLRLTGANRLVLSGGVALN
                     AVGNMRLLEHFDEAWFARFQHRKARLHLWVPPTPGDPGVTIGAAWLFAHLAGAPRGAA
                     MTHAFYCGSSPTQAEIATALEAGDIASTRIGDISTPRGRDALADLMAFAVAKNGIIAL
                     YQGAAETGPRALGHRSIFANPCDPQARERLNERVKYREAIRPLAPMATLEAAQEYFDL
                     HQGASDGNYNAYNYMVLTARSKPPARAKIPAVIHADGTGRIQIVRAEDDPLTYAYLKA
                     LGRYIGVEISVNTSFNVAGPIAQTPSQAIDTLRRSRGLDVVLLVASDGTAYAAWHGGN
                     RDSGRFTGWLSEWQTASAGR"
     gene            10266..10691
                     /locus_tag="VN968_25105"
     CDS             10266..10691
                     /locus_tag="VN968_25105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32545.1"
                     /translation="MEGDVVKLNRTAKLTGCASLVLGSFLLAPMAHAAQQGCSVEKLQ
                     GRYVFTGQGTNLHYGALDFDGAGKFLGKQTSLRDKTSIQREALSGTYHLDADCTGTMT
                     LEGQPGGTAHWDVFVTSDGKKGRMIRTDSGTMGVRTFEQ"
     gene            complement(10758..11169)
                     /locus_tag="VN968_25110"
                     /pseudo
     CDS             complement(10758..11169)
                     /locus_tag="VN968_25110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711968.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(11176..11577)
                     /locus_tag="VN968_25115"
     CDS             complement(11176..11577)
                     /locus_tag="VN968_25115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32546.1"
                     /translation="MKRVFPIVVVGLALSLGACSRDPGPKGEPGAQGPAGPQGAQGIQ
                     GVAGAQGQPGAQGAQGPQGTPGEKGAPGDKGEKGDKGEATVNLRSVQADGAASCEANE
                     TLVSVFCPSGGAPDGAKCGTSPTVGLCLRKP"
     gene            complement(11701..12588)
                     /locus_tag="VN968_25120"
     CDS             complement(11701..12588)
                     /locus_tag="VN968_25120"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL32547.1"
                     /translation="MTKVAFIGLGRMGHGMAGRYLDGGFAVAVWNRSKAKAEDLIARG
                     ARWAASPADAADGADATVTMVADDEASRAVWLGKDGAVRTAKPGTLAIECSTVSYGHA
                     LALARELRESGLVYIDSPVTGLPDAAASGNLTLLVGAEPADLERARPYLTPLSTTIRH
                     FGAVGNGTVYKLINNLMGAIQIAGIAEGLAIAEQAGLDMKLVLEAIETGVAGSPQVIR
                     HSKRMVARNFAGANFTAALRHKDAAYAVALAESLLSAVPVMGRAAVEAYDRAKAYAPD
                     DDEGKMIEIVSRAKGGNAK"
     gene            complement(12592..13723)
                     /gene="prfB"
                     /locus_tag="VN968_25125"
     CDS             complement(join(12592..13653,13655..13723))
                     /gene="prfB"
                     /locus_tag="VN968_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502756.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HXL32548.1"
                     /translation="MRAEVERLVEEIKQSVGLLRRHLDVDASTARLAELNKLAEDPNL
                     WNDPQKAQKLMQERTSLEDSLGGIGKVERELEDNIGMIELGEAESDEGVVAEAETALK
                     ALKKEVARRELEALLGGEADRFDSYLEVHAGAGGTESQDWAAMLLRMYTRWAEKHGFK
                     IEYLEETQGEEAGIKSATIQISGHNAYGWLKTEAGVHRLVRISPFDSNARRHTSFSSV
                     AIFPVVDNSIKIDIAESDVRVDTMRSGGAGGQHVNKTESAVRLTHIPTGVAVVCQAGR
                     SQHKNKAQAWDMLRARLYEIELKKREEQAAADQAAKTDIGWGHQIRSYVLQPYQMVKD
                     LRTGVQTSDTSGVLDGDLDEFMAATLAQRAFGAPPPSIEDVE"
     gene            complement(13867..16359)
                     /locus_tag="VN968_25130"
     CDS             complement(13867..16359)
                     /locus_tag="VN968_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HXL32549.1"
                     /translation="MRFLVRFIGFLFAAGTVVFLVGVAAAAGLIWHFSKDLPDYSQLQ
                     DYEPPVMTRVHASDGALLGEYSKERRLYLPIQAVPKLVINAFLAAEDKNFYEHGGIDF
                     TGMARAAVLFAQNYGSNRRPQGASTITQQVAKNFLLTNEVSFTRKIKEALLAMRLERT
                     YSKDKILELYLNEIYLGLGAYGIAAASLVYFDKSVNELTIGEAAYLAALPKAPSSLHP
                     VKNRDRAIERRNYVIDRLLENGWIKQADADKARKDPLIVTNRSNAAHTFAGEYFAEEV
                     RRDVFERYGEKKLYEGGLSVRTTLDPKLQVMARKTMAAGLVTYDEAQGWRGATSKLDI
                     SGDWGVKLADVKSLSDISPWRMAVVLETSDQSARIGFQPGRELGGAVSKERQTGNITL
                     DGVRWAKAASGPARGRTPSSVSQVLSPGDIVYADPLIAKDGSIVEGQYRLRQLPEVSG
                     AMVVMDPWTGRVLAMVGGFSFDQSQFNRATQAYRQPGSSFKPIVYSSALDNGYTPSTV
                     VVDAPIEIDQGQGGGVWRPENYSAGHYRGPTTLRNALRFSLNTVTVRLAQDIGMPLIG
                     EYAKRFGVYDELPNYLSYALGAGETTVMRMVTAYSMFANGGRRVKATLIDRIQDRYGH
                     TIFKHDARECRGCDAPGGWKNQPEPQLVDRREQVLDAMTAYQITSMMEGVVQGGTATV
                     MREVGKPIAGKTGTTNDEKDVWFIGFSPDLVVGLYLGYDKPRTLGRSAQGGRTAAPIA
                     KDFMKLALADKPAIPFKVPAGIKLITVDQMSGMRAGPGSGRTVLEAFKPGTAPPDNYS
                     VIGVADADGRTTLQPSPDADRSLFRPGTGGLY"
     gene            complement(16575..17879)
                     /locus_tag="VN968_25135"
     CDS             complement(16575..17879)
                     /locus_tag="VN968_25135"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXL32550.1"
                     /translation="MANRANNWFLLGSALLCAAALLCSGSAGVGAAEEQSPNPTAAPG
                     FPIASDARLAGDAKQTRFVLDLDKPIQFRAFTLADPYRVVVDIPQVDFRLPAEAGATG
                     RGLIQAFRYGLVMPGGSRIVFDLTGPARIAKSYVLDAANGQPPRLVLELEQVDRTGFV
                     QSLAAESRPELRPALSVAADPVVAMKTAVPEPAAAAPAADSRPVVVIDPGHGGVDNGT
                     QAGGENEKDLVLGFGLALRDRIEKAGKYRVVMTREDDTFIPLADRVTIARNQSAALFV
                     SIHADALPPGEGDAQGATIYTLSDKASDAEAERLAEAENRADAIGGVKLTEEPAEVAD
                     ILIDLVRRETRTFSNRFARQLMSEMKTSVRMHKHPLKSAGFKVLKGPDVPSVLVELGY
                     ASNKDDLEHMKSDSWRARTVGSMAQAIDVFFAKRVAKAGPTN"
BASE COUNT         3338 a         5847 c         5506 g         3475 t
ORIGIN      
        1 ggattcgttt ttcgtcatag gtgcggcttc cggtcgaagc tcgtcgaggt cagttgttgt
       61 tcggcgccgg aaattcagcc gggggcttgc cggtgttgaa ctcccagatt ttccgcgaga
      121 ctcccgggaa tatggccgaa atcggattga cgcgaagcgt cggcttgtcg gtggtcccga
      181 cgacttcgta ggttacaccg atcagcccct cattgctgcc gcccatgaac agcccgacaa
      241 tcggaatctg gcctaacatg ttgttcagcg cgtacatcgg aacgaaggtg ccgctcatgc
      301 gcacctggtt ggcggggaaa tcgatgttgc ccccgatggt gccgccgacc atcggacctt
      361 tcacgacgcc gtcccggatc gtgagttggc cgttctgcca ggtaaattcc gcgcgcaatg
      421 ccgagaaggc aacgccgttc tgcactccgg ttgacccgcc cgcagcgatg cgatcaagcg
      481 aagcctcgcc cttgaccgag aaattgcgga tgttgatcag cccgtccctc gtcttcggtt
      541 cggctgtcgg tggatccatt gtcaattgaa gctggcctcc aaccaccttg gagtaggtgt
      601 cggtaaagcg gaagaaggcg ccggcgtcat tggtctcgag atagatcacc tcgcgtccct
      661 gcgcgggcct cccgaaattt ccggtcaggg gcgtatcccg gccaagcttg ccgttgagcg
      721 cgaagctcct gatcgttcca ttgcgccgcg ataatttgac atcgaggctg cgcaccgctt
      781 cgccattgtg gccggctacc gcgccgagct tcacatcgac atcgaaatcg aggttcctgg
      841 tcttgctctt ggggtcggcc tccttgcccg acatcgcgga tttgagaaag ccacgaccgt
      901 cgaacacatc gccgcgcatc gtcactttca gcacgccatc agagccacgt tcggccttca
      961 gcgaggtctt gtcaccctcg gatggagaat aggtcgggaa gttgacgctg atcaggtcgc
     1021 cgttctgatc gacctcgagc gatcccttga tcgacacgcc gccgccatcg atgacaatgt
     1081 cttcgaagcg ggtcgattgc ggtttctgca ccacgttgaa aaccgcacgg ctcgacttgc
     1141 ccggcaactt cacccagccc ggcaggacat tgtcgagcct caacgccgtc agatcggctt
     1201 cgattcccat gcggctgtcg cgatcggggc cgccgatctt gccgatcagc ttgatcggaa
     1261 gattcccggt gacggcgggg ccgagatcca tcccgagccg cgcgcggctc gcgtcatcga
     1321 gcgtcgcctg caacttgata tccgcatcgc cgtcgttggg cttgcgataa tccagcaagg
     1381 ccggttgccc attgatcttg acgtcgccct tgacctgata tcccccgttg ttggcgacga
     1441 ccttcagctt gtcggcttcg agcttttgat tcatcaccag gcggtcggcg ccgaatccgc
     1501 caacgtcggc cgtgacggtg tagctggtgt cggccttggt cagttcgcgc ttgaccggca
     1561 ggccgagtgt aaccagggcg gcaaccgttc ccttgctcga attaggatcg atcaaggtgc
     1621 cggaaaattc gctgagacgg tcggaggcga ggatttccgc caccgccggc accgggccat
     1681 cgatccggaa tttgatgcgc gccggcgagg gttttggcgc catgtccggc acctcgaata
     1741 caaaatcggt gatgttgagt ttgcggcccg ccggggtgtc caccgccccc tgtccgatcg
     1801 tcacggtggc ggtgcggccg gtgacatgcg ctttcagatc cgcatcgcgc accgacggca
     1861 gcgtgtccac cggtcgcaat gtcacgccgc tggcgacgat gttgacggcg agaccgtcat
     1921 cgggaatcgg cggacctcgc cgcgagaggt tgcgaaccgg cgagttgacg ccaatctcga
     1981 tgcgctgcag cgagccgcgc tcgatgcgct cgatcaccca ttcgcgcacc tccggcacga
     2041 tcaggatcgg ccacatccgc ttcagcgcgg ccgccgacat cggcgttccc gcgaaaccga
     2101 gttggagacg cgcctccccc gaataatcga tgttaccggt gccagcgaag ccgatctcgc
     2161 cgttgctgat atcggcttgc gtcagcagga cgcgcttctt gtcggtgtcg aagcgcatgc
     2221 cgatggcgat gcggttgaag atcaagggtt gttcgccgtt gtcccccgca agcacgatgg
     2281 tgccgccgct gaaaccgagc tgccagtcgg tcacactgtc attgggcggc tcgagatggg
     2341 ccagcagcgt gacccggttc gcaccggaaa tgatcttgaa cggcgcgacc agcacccgcc
     2401 gtccggaatc ccattcgaca ctgatttccg ctgaatcgat cgccatcggg taatccggcg
     2461 tatcggtgtc gatgatgtgg ccttcgcccg cgatgagttt gcccgtgaag taggtcggca
     2521 gcccgtcacg gcccagttcg cccttgagtt cgccggtcag cggcaaatcc gcggtgtagg
     2581 tgaggtcctt catccgcaac gccagcagga tattcgtcgt cgaaaccttg ttggcacgaa
     2641 tatcgaccga tctcacgccg ttcgcgggcg gccccaccac gacgcgcagc gaccaggctt
     2701 tgccgccctc ctcgccgacg ctcagcgcga ccccgccgcc gctgggcctt cgaaggctga
     2761 gggtgatgtt ttcgaaattc catttgttgc cgcgctgctg atcgtcgacg atcaggtttc
     2821 cgttcttcaa cccgatttcg ttgaggttct gtccgtcaag tccggtcagg ctcaggctgt
     2881 cgagccaatc gagaccggcc aaaagcccgc ttggcgtatc gggacccgcg gcgggcgccg
     2941 gtgaggacgg agcagcagtt ccgggtggtg cggcagggat ttggcgcgga aacgtcgccg
     3001 gaatgcctgc ctgtttcttt gaggtcacac cggtagccaa aggtttggcg gtgtcaccgg
     3061 cggaaactgt cacataaccg tccggggtga tgcgcaccgc gagttcggcg tccaccagat
     3121 tgagactttc agcgcgaagc tgtcccatca gcagcgcggt gcctgaaagc cttacctcgg
     3181 ccttcggcgc gctcgccacg atgacgtgat cgcgatcgcg gacgatgata tcgcggatac
     3241 gcacggcgat gcggatccgg ccggcgcgct cgatctgcgt cccgccgacc tcgacggtgt
     3301 tgccgtgacc gatgttttcc tcgatcgccg cggccagcca cggcgtggcc atctcaagat
     3361 tgatcggacc cgcgcccagc ctccaccaca gcccgccgaa gcaccctgcg aagatcacca
     3421 tcaagaccgc gacgacgatg gcgatccgtt tgacccagcg ttcggtggcc agccaccggc
     3481 gcagcgctcc ggcatagtcg ccggcccggt gaatcccgga agccggattg gacaacagcc
     3541 ggcgcgcgcg atggcccgcg gcatcgtcat ggtcttgatc ccaaccggca tcatcccact
     3601 gcgagagccg gacgtcagca cgtggtttcg atcccttggg cgacgtgttc cttgccattg
     3661 cctctcgttg cgggcgctgg ttgtaagatt gatcgccgcc aaagccgcgc ccgccgacag
     3721 gtaacgagcg cttcggtgcc ggcatcgctg ctgccaatcc ctcgaataat actgtaactc
     3781 ttcatcaggc ccaaggaacg catttcaacg cgcggatggg tctggttgca accatactcc
     3841 aaaggccatc aaacttgccc agcagtcagc ctgattccag cacgctggga taaagcccgc
     3901 aatactccca tgattgatcc gccgaaagcg acgaaaggaa ggcgtatgtc caagaaaacg
     3961 cccaagaatt cctccaaacc ctcctccggc aagacgaccc gcgtccaagc caagatttcc
     4021 ggcaagacgc cgctgaaaac ccggaacgca acggcaaaaa aatcaggcaa gcgcgcagac
     4081 aagcaatccg gcagcgccgc cgcgcgagca tcacacaagg ccccatcaaa gaagtcaaaa
     4141 tcgccgcggg ccggcgaatc aaccttgcgg gccgcggccg aatccaaggt cgccggtgcc
     4201 aagcccaagg caaaaagtcc ggctgcgagc aaaccatcag gccggagacc cgacgcgtcc
     4261 gccggcctta aatctgaatt ggccgagggc acaaaggcgc ccgccttccg cctgccgcgc
     4321 gatggcggcg aaaccgtttc gctggcggac tattcgggta aaaaactcgt gctgtttttc
     4381 tatccacgcg cggacacccc gggctgcacc agggaggcca tcgacttcac acggcttgcc
     4441 gacgcctttg cggaaagtca gaccgcaata gtgggtgttt cggccgatcc cctgagggcg
     4501 caggaagcct tccgggacaa gcaccaactc tccgtgcccc tggtttcaga tgaagcgcat
     4561 gagatgctgg agagctacgg cgtctggggc gaaaagtcca tgtatggcag gaccttccaa
     4621 ggcatccttc gcaccacggt tttgatcggc gccgacggcc gaatcgccag aatatggcgc
     4681 aacgtaaagg tcgacggcca cgctgatgac gtgcttgccg ccgcccgtgc cctctaattc
     4741 gagcgatccc attcgctgaa aattaaccat ggcaaggtca aatcacaccc gcaaatttgg
     4801 ccgtacggaa ctcctatccg accggcgcgg gagtgccgat gtcgaaccgt tccggtcatt
     4861 gttccgaaca tacccagcac catcatcaac acgaccatgc aaaggcagcc ttgcgccgcc
     4921 cggcgacgag ccaggcgtcg gtcccttccc gttcacaacg cgcggcggag gccggctata
     4981 ccctcgtcca cgccggcaag caggtccggt ttggtccggt ggtgttctgg atcgtggtcg
     5041 gcacggtgac catgctcggc atgtggtcgg ccgcgaccgc gacctatttc gcatttcgcg
     5101 acgatgttct cacccggctg atcgcacgcc aggcggagat gcaatacgcc tacgaagacc
     5161 gcattgccga actgcgcgcc aaggttgatc gcagcaccag ccgccaaatg ctcgatcagg
     5221 agcaattcga ccagaaactc gaccagatca tgcgtcgcca gaccgcgctg gaatcgcgcg
     5281 ccactgcgct tggcgccatt ccggacgcct cggttaccgg atcgatccgg gcgccctcgc
     5341 gaggcgccgc ggccaccgac gccgccgctt cgtcggggac cccaaagccc tcgccgatca
     5401 gcgataccgt gatcttcgtg gcgcccccgg atcgtgaagc gcgcctggaa tcgcgtgccc
     5461 cggtcatcgt caatccccga tccagccagt tcgccaaggt ccagggcgtc gatagcgttc
     5521 tggcccgcct gcagggttcg ctcgatcaga tcgaaggccg tcagatagcg gcgctcagtt
     5581 cggtcgaaga gagcatggaa tcgcgtgttc gccggatgcg cggcgtcttt accgatctcg
     5641 gcctcaatat ggcgcaattg gaagccgcga ctccccgcgc gggcgtcggc ggccccttcg
     5701 tccccgtcaa gcttcccgcc aacgccggcg ctttcgagcg ccagctttac cgaatcaaca
     5761 ccacccgcgc ccaggtcgag cgtctcaacc gcaccctggc gctggtgccg tatcgcaagc
     5821 cggtggtcgg cgaagtcgaa ttcacctcag gattcggggt tcgcagcgat ccgttcctcg
     5881 gccgcccggc catgcacacc ggtctcgatt tccgcgccgc gatgggcgac ccggtgcggg
     5941 cgaccgcgaa cggaaaggtg gtttcgtcgg gatgggctgg cggctatggc cgcatgatcg
     6001 aaatcgacca tggcaacggg ctctcgaccc gctacggcca tctgtcggag atcggcgtca
     6061 aggttggcga tcagatcaga atcggacagg tgatcggcgc ggtgggctca accggcagat
     6121 cgaccggtcc gcatttgcac tatgaaacca ggatcgacgg cgacgccgtc gacccgcaga
     6181 aatttttgcg cgcgggggtg cggctcagcg ccggctgatc agcataggaa tccttgagtt
     6241 agtagggtca ccgactctaa tatccgttca tgaccgcgtt caaaagcctc atgcttgccg
     6301 gcgtcgcact cgcaatctcg gccggcgtcg ccccccgtgc ttacgcacaa acaggctcta
     6361 ccgagcttcc gcctgacgta accgcgttcg tcagccgtcg cgccagttgt tcggagtggt
     6421 ccaaaaaggc aattgatcct gggtggacag ctaagatcga agccatttat accaatctgc
     6481 aatccctgaa gtgcttcgac attatgaatg acgagcgcgc gcttcgacag aaatacgccg
     6541 gcaatccaga aatccgggca tcacttggtg ctggcgtcat cactaagttc gtcacacgat
     6601 tgccagttcg gattgctgtt ccgccagctt tggaccgcta gtgtcccacc tcgccatcga
     6661 tgacgtcgcc gaacagttcc caggtctcgc cgttgaactt catcaactgc atctgctcga
     6721 tcggaaagaa gtcatccgcc gacgtattga ccttgatacc cggcagcatc atgtcggtcg
     6781 agaagtcctt gaggttcgcg gcctgcttca tcacgttctc gcgcgtgaga tcatcgccgc
     6841 actgtttgag aacctgcacc atcgcctgcg cctggacata gccgtaggtg ttgttggcgt
     6901 tggtcttgtc gccgtcggga taatatttgt ccatgaaagc ctgccaggtc ttcacgcccg
     6961 ggtcctcctt ccaggcgaga tcggtcgggt ctttcagata cgcggtcgag ataatgtcct
     7021 ttgaatattg aaggcccgcc ggtttgagga ccgcagccac cgatgtagcg gtgttggcga
     7081 gaaagaactt cggtttccag cccagttcac cgaccttacg gatcgcctgc gccgatccct
     7141 tcggcgccgc ccacgagaag aagatgtccg cgcccgaatc gcgcagcgcg acaatctgtg
     7201 aatcgatggt ggggtcgctg acctcatagg atttatcggc gatgatcatg ctgacctttt
     7261 cgcccaaccc gtcgcgcaac cccttgacct gatccttgcc ggcgtcgtcg ttctgccaga
     7321 acaccgcaat cttgccgttg ggatagtgat cgagaatgta tctggcgtag atgcgcccct
     7381 cgctctgata attgggctgg aagcccatgg tccacggaaa attcttcgga tctccccatt
     7441 tggtggcgcc ggacgcgacg aacagctgcg gcactttctt ggcattcatg tatttctgaa
     7501 tcgccgaatt ggacggcgtg ccgagcggct gaaagagcag caaaacctcg tcgccttcga
     7561 cgagcttgcg cgcctgctcg atggctttcg gcgggctgta ggcgtcgtcg taggagatga
     7621 agttgatctt gcggccgttg atgccgccct ccgcgttgat catgttgaaa taggcggctt
     7681 ccgtcttgcc gatcacgcca taggaggaag caggaccgct atagggcatg atattgccga
     7741 tcttgatctc ggtatcgctt gcaccgggat cgtatttctt ttccgccgat gctgcggtcg
     7801 cggttagcgc ggcggcggcc gcgaaacccg ccaaggcaac aaggctcctc atccaaatcg
     7861 gcatcgttct ctcccgccgt ctattttgta ttttgcacaa gtgtcgcaag tgatgcgcag
     7921 tcatgcaagc gtcggtttat tccgcctgac gaaacagcat tgttgcgcgc cgccaattat
     7981 gattgatgca tggcagtatc tgacatccac gaggccggcc ggattgaacc cgaaactgcg
     8041 aattggtccg cgacatccga ggctcggcag aatgggattt cacgccgcgc gatggctcgg
     8101 cgcgcgtctg ttgggggccg ccggctttca tcaactcggc tcggattttg ccaatgcgcg
     8161 gattgcgtgc gtccgcgaaa agctcggccg tggcgagacg gtttatctgg ccggaatcgg
     8221 gcctcccggc acgcacaatt caggcgtcgc actggttgag gtgagccagg ccaacggccc
     8281 gcgcctgatc gtcaacaatg aggaagaacg cttttccggc aacaagcata cgaccgaata
     8341 tcctgttgcg tcgatcgatg cgatggttgc gacgctgcgc ggcatcggcc gcgatatcgg
     8401 ggatatcgac gcgtggctga ccagttggaa ttatcctgat ctggccggca cgctggtccg
     8461 ctcggtgctg gaggagttgc cgcaaagcgt gaggctgctg cgcaccaccg atgccgccgg
     8521 atttgacggc cgccgactcg atcaaatgac ccgcacgccg aaaattctcg gcaggcagct
     8581 cggcctcccg gagagagtgc cgctgatctg catgccgcac cacgacaatc acgcgtggtt
     8641 ttctttcgca gcgtcgccgt tcgcctgtga tcatgacccc gtcgccatcg ccgtgcttga
     8701 cggcaccggc gacctcggat cgatctcgct atatgtcgcc gaagccggcg tcatgcggcg
     8761 gctctactgc aatgacagcg tgttcgactc gctcggcgcc ttctacagcg tggtttcatc
     8821 gacccagggc ggctggacct ggctgtcgag cgagggccgc tacatgggcg ccgccgcctg
     8881 gggcgacatg aatcgcgcca gcaaccccta ttacgcgcgt ctgaaggacg tgctgcattt
     8941 cggcagcaac ggcgaggtgc gcctcaatcg cgcggttgcc aactggtatt gcgacccgtt
     9001 cgatcatccc tacaagcccg cgctgatcga aatccttggg ccaccgttgc ggcccgatca
     9061 gctctggaat cccgacgcgg tgctgcgggt cgaggatatc aaccaccggc ccgacaccaa
     9121 ggaccgcctc gacaaggccg cagccacgca actggtgttc gaagatgcca tgatccacgt
     9181 ggtcgatcat ctcctgcgct tgaccggcgc gaaccggctg gtgctgtcgg gcggcgtggc
     9241 gttgaacgcg gtcggcaaca tgcggctgct cgaacatttc gacgaggcgt ggttcgcaag
     9301 attccagcac cgcaaggccc gcctgcatct gtgggtgccg ccgacgccgg gcgaccccgg
     9361 cgtcacaata ggcgcggcct ggctgttcgc gcatctggcg ggcgcgccgc gcggcgccgc
     9421 gatgacgcat gcgttctatt gcggttcatc gccaacgcag gcagagatcg caaccgcgct
     9481 cgaggccggc gacatcgcct cgacccggat cggggatatc tcgacgccgc ggggccgcga
     9541 tgcgctggcc gatctgatgg cgttcgcggt ggcgaaaaac ggcatcatcg cgctgtatca
     9601 gggcgcggcc gaaaccgggc cgcgcgcgct cggccaccgc tcgatctttg caaatccttg
     9661 cgatccccag gcgcgcgaac ggctcaacga gcgcgtcaaa tatcgcgaag cgatccgtcc
     9721 gctggcgccg atggcgacac tggaagccgc gcaagaatat ttcgatctgc atcagggcgc
     9781 gtcggacggc aactacaacg cctacaatta catggtactg accgcgagat cgaagccgcc
     9841 ggcgcgcgcc aaaattccgg cggtgattca tgccgacggc accgggcgga ttcagatcgt
     9901 gcgggcggaa gacgatcccc tcacctacgc ctacctgaag gcgctcggcc gctacatcgg
     9961 cgtcgagata tccgtcaata cgtcgttcaa tgtcgccggc ccgatcgcgc agacgccaag
    10021 ccaggcgatc gacacgctgc gccgatccag ggggcttgac gtggtgctgc tggtggcgag
    10081 cgacggcacg gcctatgcgg catggcacgg cggaaatcgc gacagcggcc gctttacggg
    10141 ctggctctcg gaatggcaga ccgcatcggc gggccggtga tgatctgtgc gcggcgggag
    10201 tcagcgccgc attctttctt gttgttgccg caatcctgtc gtgctgttcc agccgctgca
    10261 acgggatgga gggcgatgtt gtgaagttga acagaaccgc gaagctgaca ggctgtgcaa
    10321 gcctggtgct aggaagtttc ttgctggccc cgatggccca tgcggcacaa cagggatgtt
    10381 cggtcgagaa gcttcaaggc cgctacgttt tcaccggcca aggtacaaac ctgcattatg
    10441 gcgcgttgga cttcgacggc gcgggaaaat ttttgggcaa gcagacctcg ctgcgcgaca
    10501 agacatccat ccagcgcgaa gccttgagcg gcacgtatca tctggatgcg gactgcacgg
    10561 gaaccatgac cctcgaaggt caaccgggcg ggacggcgca ttgggacgtc ttcgtcacca
    10621 gtgatggcaa gaaggggcgc atgatccgga cggattccgg tacgatgggc gtccgcacct
    10681 tcgaacaata gcggcggctc taaccggcgg ctccgttcct tgcggtcggg gccgcgctct
    10741 ggtcgccggc gaaggcgcta tcgcgttgcg ggcacgcgcc ggacgaaatt gagcacgcgc
    10801 ccgtcgatca gcagcagcgc gctgccggtc acgagggcgc cgacgatctc gcgcagcgag
    10861 atgctttcgc ccagcacgag atagccgagc agaatagccg tcaccggaac gagaagcgtc
    10921 accagcatga cgttggtcga gcccgagcgc tgcaacacgt ggaaaaatac gatataggcc
    10981 agcgcagtcg acaatccggc gaggcccatc atcgccagcc acgtcgtttg cgcccggcat
    11041 cggcaattgc cacggacgtt cgaagaccgc cgcgatgatg gtcatcatca gactcgacgc
    11101 cagcaattga aacgtcgccg tcgcaagcgg cggcgagttc gacagcttac gccgtgcaca
    11161 gagtgcggcc agattttaag gcttgcgcaa acaaaggcct acggtcggtg acgtcccgca
    11221 ttttgcgccg tccggcgcgc caccgctcgg acagaacacc gacaccaggg tctcattggc
    11281 ttcgcagctt gccgccccgt ccgcctggac cgacctcagg ttgaccgtcg cctcgccttt
    11341 gtcgcctttt tcgcccttgt cgccgggtgc gcctttttcg ccgggtgtac cttgcggccc
    11401 ctgcgcacct tgcgcgccag gctgtccctg agcgccggcc accccctgaa tgccctgcgc
    11461 accctgcgga ccggccggac cctgtgcgcc cggctcacct ttcggccccg gatccctgct
    11521 gcaggcgcca agcgaaagtg ccaacccaac gacgactatg ggaaaaactc tcttcatgat
    11581 ccccccgcaa tgaatgattg tgtcaccggc tgcacgaaag gcagcatcaa gacaaggccg
    11641 ccgaccgcgt cagttcggcg acgttaggta tcggagtgaa gcgatgcaat agcggcggcg
    11701 ctatttcgcg ttgccgccct ttgcccgcga cacgatctcg atcatctttc cttcatcgtc
    11761 atcgggcgca taggccttcg cgcgatcata ggcctcgacc gcggcgcgtc ccatcacggg
    11821 aacggcggac aacaggctct cggccaacgc gaccgcatag gcggcgtcct tatgccgcag
    11881 cgcggcggtg aagtttgcgc cggcaaaatt ccgcgcgacc atccgcttgg aatgacggat
    11941 cacctgcgga ctgccggcaa cgccggtctc gatcgcttcc agcaccagct tcatgtcgag
    12001 gccggcctgc tcggcgatcg caaggccctc cgcgatgccg gcaatctgaa tcgctcccat
    12061 cagattgttg atcagcttgt agacggtgcc gttaccgacg gcgccgaaat gccggatcgt
    12121 cgttgagagc ggcgtcagat agggccgcgc gcgctcgaga tcggcgggct cggcgccgac
    12181 cagcagcgtc aagtttccgc ttgccgcggc atccggcaaa ccggtgaccg ggctgtcgat
    12241 atagaccaga cctgattcac gaagctcgcg cgccagcgca agcgcgtggc cgtacgacac
    12301 ggtcgaacat tcgatcgcca gcgtgccggg tttggccgtc ctgacggccc catccttgcc
    12361 gagccagacg gcgcgcgacg cctcgtcgtc ggcgaccatg gtcacagtag cgtcggcgcc
    12421 atcggctgcg tccgcgggtg aagccgccca tcgcgcgccc cgcgcaataa gatcctcggc
    12481 tttcgctttg ctgcgattcc agaccgcgac cgcaaagccg ccatcgagat agcggccggc
    12541 catgccgtgc cccatgcgcc cgaggccgat gaaggcaacc tttgtcattt gttactccac
    12601 atcctcgatg gaaggcggcg gagcgccgaa cgcgcgttgc gccagcgtcg ccgccatgaa
    12661 ttcgtcgagg tcgccgtcga gcacccccga ggtatcggac gtctgcacgc cggtgcgaag
    12721 atccttcacc atctgatagg gctgcaggac ataggagcgg atctggtggc cccagccgat
    12781 gtcggtcttg gcggcctggt cggcggcggc ctgctcctcg cgcttcttca gctcgatctc
    12841 gtagagccgc gcgcgcaaca tgtcccacgc ctgcgccttg ttcttgtgct gcgagcgtcc
    12901 ggcctgacag accaccgcaa cgccggtcgg aatgtgcgtg agccttacgg ccgactcggt
    12961 cttgttgacg tgctgcccgc cggcgccgcc cgatcgcatg gtatcgacac gaacgtcgga
    13021 ctcggcgatg tcgatcttga tcgagttgtc gaccacggga aagatggcga cgctggaaaa
    13081 cgaggtatgc cggcgcgcgt tggaatcgaa cggcgatatc cgcaccagcc ggtgcacgcc
    13141 ggcttcggtc ttcagccagc cataggcgtt gtgaccgctg atctggatgg tggcggattt
    13201 gatgcccgcc tcttcgcctt gggtctcttc gagatattcg atcttgaagc cgtgcttttc
    13261 cgcccaacgc gtgtacatcc gcagcagcat cgcggcccag tcctggctct cggttccgcc
    13321 ggcgccggcg tgaacctcga gataggagtc gaaccggtcg gcctcgccgc caagcaaggc
    13381 ttcgagctcg cggcgcgcga cttccttctt gagggccttc agggcggtct cggcttccgc
    13441 caccacgcct tcatcgctct cggcctcgcc gagttcgatc atgccgatat tgtcctcaag
    13501 ctcgcgctcg accttgccga tgccaccaag cgaatcctcc agcgaggtcc gctcctgcat
    13561 cagcttctgc gctttctggg gatcgttcca gagattgggg tcttcggcga gcttgttcag
    13621 ctccgcgagg cgcgccgtgg atgcatcgac gtcaaagatg cctcctcagc agcccgaccg
    13681 actgcttgat ctcttcaacg aggcgttcga cttcagcgcg catgggttct ctgatctgcg
    13741 gcacgaagcc gccgttatca atgcttcgag ggatgtagcg gcgcgcgcca caaagcgcaa
    13801 cgctcgagac gcgaaccggt ttccaccccg gatcaagtcc ggggcggact ttcgcttgaa
    13861 aactctctag taaaggccgc ccgtgcctgg acggaacagg ctgcgatccg cgtcggggga
    13921 tggctgcagc gtcgtccgcc cgtcggcatc ggcaacgccg atcacggagt aattatccgg
    13981 cggcgcggtg cccggcttga aggcttcgag aaccgttctt ccactgcccg gaccggcccg
    14041 cattcccgac atctgatcga cggtgatcag cttgataccc gcaggcacct tgaacggaat
    14101 cgccggcttg tcggcgagcg cgagcttcat gaaatccttg gcgatgggcg ctgcggtgcg
    14161 gccaccttgt gcgcttctgc ccagagtgcg gggcttgtcg tagccaagat agagaccgac
    14221 cacgagatcc ggcgaaaagc cgatgaacca gacatctttt tcatcattgg tggtgccggt
    14281 cttgccggcg atcggcttgc ccacttcgcg catcacggtc gccgtgccgc cctggacaac
    14341 gccttccatc atcgaagtaa tctgataggc cgtcatcgca tccagcacct gttccctgcg
    14401 atctaccagc tgcggctcgg gctgattctt ccagccgccc ggcgcgtcgc agccgcggca
    14461 ttcgcgcgcg tcatgcttga agatggtatg gccgtagcgg tcctggatgc gatcgatcag
    14521 ggtggccttg acccgccggc cgccgttggc gaacatcgaa taggccgtga ccattcgcat
    14581 caccgtggtc tcgccggcac cgagcgcgta cgacaggtaa ttcggcaact cgtcatagac
    14641 cccgaagcgc tttgcatatt cgccgatcaa gggcatgccg atgtcctgcg ccaaccgcac
    14701 cgtcaccgtg ttaagcgaaa atctcagcgc gttacgcaag gtcgtggggc cacgataatg
    14761 gccggccgaa tagttctccg gacgccagac gccgccgcct tgcccctgat cgatttcgat
    14821 cggcgcatcg accacgaccg tcgatggcgt atagccgttg tccagcgcgg acgagtacac
    14881 gatgggtttg aaggaggatc ccggctgccg gtaggcctgc gtcgcacggt tgaactggct
    14941 ctggtcgaac gagaaaccgc cgaccatcgc cagcacgcga ccggtccagg ggtccatcac
    15001 caccatcgcg ccggaaacct ccgggagctg gcgcagccgg tactggccct cgacgatact
    15061 accatctttt gcaatcagcg ggtcggcata aacaatgtcg ccggggctta gcacctgcga
    15121 aaccgatgac ggcgtcctgc cgcgtgccgg gcccgaggcg gcctttgccc atctgacccc
    15181 gtcaagcgtg atgtttccgg tttgccgttc cttcgaaacc gcgccaccca attcacggcc
    15241 cggctgaaaa ccaatccggg cggactggtc gctggtctcc agcaccacgg ccatccgcca
    15301 gggcgagatg tccgagagcg atttgacgtc ggcaagcttc acgccccaat cgccggagat
    15361 atcgagcttg ctggtagcac cgcgccagcc ctgcgcttcg tcataggtga cgaggccggc
    15421 cgccatggtc ttgcgcgcca tcacctgcag cttcggatcc agcgtggtcc gtaccgacag
    15481 gccgccttca tagagcttct tttcgccgta gcgctcgaac acatcgcggc ggacttcctc
    15541 ggcgaaatac tcgccggcga acgtgtgcgc ggcattgctc cggttggtca cgatcagcgg
    15601 atccttgcgg gccttgtcgg catcggcctg cttgatccag ccattttcga gcagccggtc
    15661 gatcacgtaa ttgcggcgtt caatggcgcg gtcgcggttc ttcaccggat ggagcgatga
    15721 cggagccttg ggcagcgccg ccaggtaggc cgcctcccca atggtaagtt cattcaccga
    15781 tttgtcgaaa tagaccagcg aggccgcggc gatgccgtag gcgccgaggc cgagataaat
    15841 ttcattgaga tagagttcga gaatcttgtc ctttgagtag gtgcgctcga ggcgcatcgc
    15901 cagcaaggct tccttgatct tgcgggtgaa cgacacctca ttggtcagaa gaaagttttt
    15961 ggcgacctgc tgggtgatcg tggaagcacc ctgcggccgg cggttcgagc cataattctg
    16021 cgcaaagagc accgcggcgc gcgccatgcc ggtaaagtcg atgccaccat gttcatagaa
    16081 gttcttgtcc tcggccgcca ggaacgcgtt gatgaccagt tttggaaccg cctggatcgg
    16141 cagatacagc cggcgttcct tggaatattc gcccagcagc gcgccgtccg aggcatggac
    16201 acgcgtcatt accggcggct cataatcctg aagctgcgaa tagtccggca aatccttgga
    16261 gaaatgccag atcagccccg cggccgcggc cacgcccacc agaaacacaa cggttcctgc
    16321 ggcaaacaag aaacctatga accgcaccag aaaacgcatg ataaaagttc cgtttcagcc
    16381 ccgcgccatc ataaccggcg ccatccccag cgtgaagagt gcttcggatc gatttgcgat
    16441 tccgcgaatg cgcgcaatac attggccgcg atggtctttt gatcgattcc gaagacgtcg
    16501 cggtagcttt ttattttcta tagggccgtc gctgtggcca aactagggct ttccacaatc
    16561 cgaccgctcg gtgttcaatt cgtcggccct gccttcgcca cgcgtttggc gaagaaaacg
    16621 tcgatcgcct gcgccatcga tccaacggtc cgggctcgcc agctgtccga tttcatgtgt
    16681 tcgagatcat ccttgttcga ggcatagcca agctcaacaa ggaccgacgg cacgtcgggg
    16741 cctttcagta ccttaaaccc ggccgatttc agcgggtgct tgtgcatccg tacggacgtt
    16801 ttcatctcgc tcatcagctg gcgcgcgaat cggttcgaga aggtgcgggt ttcgcgccgg
    16861 accagatcga tcagaatgtc ggccacctcg gcaggttcct cggtgagctt gaccccgccg
    16921 atggcgtcgg ccctgttttc cgcctcagcc agccgttccg cctctgcgtc ggaggccttg
    16981 tcggacaggg tgtagatcgt ggcgccctgg gcatcgcctt cgccaggcgg cagcgcgtcg
    17041 gcatgaatcg acacgaacag cgccgccgac tgattgcggg caatcgtgac ccggtcggcg
    17101 agcggaatga aggtatcgtc ctcgcgggtc atgacaacac gatatttgcc ggctttctca
    17161 atgcgatcgc gcaaggccag cccgaatccc aacaccaggt ccttttcgtt ttcgccgccg
    17221 gcctgcgtgc cattgtcgac gccgccatgg cccgggtcga tcacgaccac cggacgcgaa
    17281 tccgccgccg gcgccgccgc ggcgggttcg ggcacagccg tcttcatcgc gacgacgggg
    17341 tctgcggcga cggagagggc tggtctcagc tctggccggc tttcggcggc gagcgactgg
    17401 acgaagccag tgcgatcgac ctgttccagc tcaagcacga gccgcggagg ctggccgttg
    17461 gccgcgtcca gtacgtagga ttttgcgatc cgggccgggc cggtcagatc gaacacgatc
    17521 cgggagccgc ccggcatcac caggccatag cggaacgcct ggatgagccc gcgcccagtg
    17581 gcgccggctt cggccggcag tcgaaagtcg acctggggaa tatccactac cacccgatac
    17641 ggatcggcga gcgtaaaggc gcgaaactgg atgggcttat cgaggtcaag aacaaaccgg
    17701 gtttgcttgg cgtcaccggc caggcgcgcg tccgaggcga tcggaaagcc cggggcggcg
    17761 gtcgggttcg gcgattgctc ctcggccgcg ccgacccccg cggacccgga acacagcaat
    17821 gctgcagcgc acaatagcgc gcttcccagc aaaaaccaat tatttgcgcg gttcgccaac
    17881 gaatctgtgc ctccgagcag ccctcttacc gcaataggac cgcagggtta actgcagctt
    17941 aaaactgttc cccaaataag gccaagtgtt gccgcgcagc gacgcttccc ttgcgcggag
    18001 cggccaatcc acgtatgtaa aaggagctga cggctaaaac tcccggttgt gtcgcgttca
    18061 gcctcccggt gcagcgccgg agccttcgaa acttcggaga ttccagcgtc tttccgatcc
    18121 ctccgcagcc agcgcagcgc gcggctgaca aggagcggcg gtttcg
//
LOCUS       DATNOI010000620         3003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_188756,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000620 DATNOI010000000
VERSION     DATNOI010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188756"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..317)
                     /locus_tag="VN968_25140"
     CDS             complement(189..317)
                     /locus_tag="VN968_25140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32551.1"
                     /translation="MTHWHDSMVDRPETDGAVYMAGWTISGLAVLGTIVAVWILGI"
     gene            complement(454..699)
                     /locus_tag="VN968_25145"
     CDS             complement(454..699)
                     /locus_tag="VN968_25145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32552.1"
                     /translation="MSVISRAPRVTFAGSSTDSNLAMSLGIPAVTIGGGGEGGNWHSR
                     NEWYKPVDAYYGPQNALLTILMLTGLDGVTKPALQVR"
     gene            complement(716..1549)
                     /locus_tag="VN968_25150"
     CDS             complement(716..1549)
                     /locus_tag="VN968_25150"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXL32553.1"
                     /translation="MRISLTRSILAAALLLAAGPLAKQAYAQSAAAGSAVEAAYQAIL
                     TSPKVIKTLEDIKADDATAFAEQKRITEIPAPPYKEKVRAEYYLKRFQELGFKDAAID
                     SEGNVIGLRKGSGGGRPKLVVSAHLDTVFPEGTDVSVKEKDGVILAPGVGDDSRGLAA
                     LLSLIKSINANEIATVGDVMFVGTVGEEELGNLRGVKALFRDHADIDGFISIDGLGIT
                     RVVNQATGSHRYEMIFKGPGGHSFQEFGLPSAIHAMGRAIAKISDLQTRRHRRAGFAD
                     R"
     gene            complement(1568..2080)
                     /locus_tag="VN968_25155"
     CDS             complement(1568..2080)
                     /locus_tag="VN968_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32554.1"
                     /translation="MQTELNKVVTALADFYAHETYSFEKDLGERTLTHRLAVHLEKQF
                     SGWSVDCDYNRLGERTLRLPHGTMVSTDDHFAKSVYPDIVVHQREIPNNLLAIEVRKA
                     ANHQSAEHDQHKLRALTDPHLWFAYWIGVYLVLGKNSVVSDVYSGGVIDQPMSLWFSQ
                     RLKEVGLGLS"
     gene            complement(2106..>3003)
                     /locus_tag="VN968_25160"
     CDS             complement(2106..>3003)
                     /locus_tag="VN968_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HXL32555.1"
                     /translation="AAQIAGKTGCKVMGQTYNPRMARGRGRFSIDRIPYVIEQALPIL
                     KDFRHIILVETNDPVAFFAYPNKPSLLKPEGCEVHRMTSGGENSVAALEALAYAVGAK
                     PADVKPQPLVEIAKPTGALTYAAIAQAIAMVIPENAIVVDESITSGRGFFPPTAAAAP
                     HDWLQNMGGSIGFSTPVATGAAVACPDRKVICMVGDGSAMYTLQSLWTQARENLNVTT
                     IVFANRIYQILRGEFDGVGAGEPGQRALDMLKIDRPAIDWVSLAKGMGVQGRAVATAD
                     ELNKALAEAIPEKGPRLIEVQM"
BASE COUNT          543 a          957 c          900 g          603 t
ORIGIN      
        1 ttcgcggtgg gacttctgat cggacgcaga ctgggattgc tcgtcactgg ccgcaagcgg
       61 gttttgcaaa gatagttgga gccgtttttt tgttcgagca ggagcgtttc gaacgactta
      121 gcccgtaacg cggacgagcg aacggcatca agccgccaca tccatcggcg ccgagattcg
      181 tttccggatc aaatcccgag aatccaaacc gcaacaatgg tgccgagcac ggccaggcca
      241 gaaatcgtcc agccagccat gtaaaccgca ccatccgtct ccggcctgtc taccatggaa
      301 tcgtgccagt gggtcatgac agcctcctgg agattgcctc tggtaggtaa gtctccgcca
      361 gcggctgaag gttcaaacgg aataatcgcg gctttgttcc gataattggg agcgccggag
      421 agacggcgcc ctgccctccc gcccttactt cccctacctc acctgtagcg ccggcttcgt
      481 caccccatcg agccctgtca gcatcaaaat cgtcagcagg gcgttttgcg gcccataata
      541 ggcgtcgacc ggcttgtacc attcgttgcg cgagtgccag ttgccgccct cgccgccgcc
      601 gccgatggtc acggcgggaa tgccgagcga catcgccaaa ttggaatcgg tgctggagcc
      661 ggcaaaggtc acgcgcgggg cgcggctgat gacggacacg gcgcgcttcg tggcctcaac
      721 gatcggcgaa tccagcgcga cgatgccggc gggtctgcag atccgagatc tttgcaatgg
      781 cgcggcccat ggcatggatc gcgctcggca atccgaactc ctggaaactg tggccgccgg
      841 ggcccttaaa aatcatctcg tagcgatggc tcccggtcgc ctgattgacg acgcgggtaa
      901 tgccgagccc gtcgatcgag atgaagccgt cgatgtcggc gtggtcgcgg aacagcgcct
      961 tgacgcccct gagattgccg agctcttcct cgccgacggt gccgacgaac atcacgtcgc
     1021 cgacagttgc gatctcgttc gcgttgatgg acttgatcag cgacagcagc gcggcgaggc
     1081 cgcgggagtc atcgccgacg cctggcgcga ggatgacgcc gtctttctct ttgaccgata
     1141 cgtcggtgcc ttcgggaaac acggtgtcga gatgcgccga caccaccagc ttcggcctgc
     1201 cgccgccgct gcccttgcgc aagccgatca cgttgccttc gctgtcgatg gcagcatcct
     1261 tgaagcccag ctcctgaaac cgcttcagat aatactcggc cctgactttt tccttgtagg
     1321 gcggcgccgg gatttcggtg atgcgcttct gctcggcgaa tgccgtggcg tcatcggcct
     1381 tgatgtcctc gagcgtcttg atcaccttgg gactcgtgag aatggcttga taggcggctt
     1441 cgactgccga tccggcagcg gcgctttgcg cgtacgcttg cttggccaac ggtccggccg
     1501 ccagcaggag cgcggccgca aggatggatc gtgtgagcga gattctcatt tgcagactcc
     1561 gcatctgtca gcttaacccc aaccccactt ccttcagccg ctgggaaaac cagagggaca
     1621 tgggttggtc gatcacgccg ccgctgtaca catccgatac gacactgttc tttcccaata
     1681 caagatagac cccgatccag taggcgaacc acagatgcgg atcggtcagc gcgcgcagct
     1741 tgtgctggtc atgttccgcc gactgatgat tggctgcctt cctgacttcg atcgcgagca
     1801 ggttgttggg aatctcgcgc tggtgcacga cgatatcggg atagaccgat tttgcgaagt
     1861 gatcgtcggt agagaccatg gttccatggg gcagccgcaa cgtgcgctcc cccagcctgt
     1921 tataatcgca atcgacgctc cagcccgaaa attgcttttc cagatgcacc gccaggcggt
     1981 gggtcagcgt gcgctctccg agatccttct caaacgagta ggtctcgtgc gcataaaaat
     2041 cggcgagcgc agttacaacc ttgttcagct cggtctgcat gttgtctccg acgaaaacgt
     2101 gccctctaca tctgcacctc gatcagccgc ggccccttct caggaatagc ttccgccagc
     2161 gccttgttta attcgtcggc ggtagcaacc gcgcggccct gaacgcccat gcccttggcg
     2221 agcgagaccc agtcgatcgc cgggcggtcg attttcagca tatcgagcgc gcgctgtccc
     2281 ggctcgccgg cgccgacgcc gtcgaattcg ccccgcagga tctggtagat gcgattggcg
     2341 aacacgatgg tggtcacgtt gaggttttcg cgagcctgcg tccacagcga ctgcaacgtg
     2401 tacatcgcgc tgccgtcgcc caccatgcag atcaccttgc ggtcgggaca tgcgaccgcc
     2461 gctccggtcg cgaccggcgt cgaaaatccg atcgagccgc ccatattctg cagccagtcg
     2521 tgcggcgcgg cggccgcggt cggcggaaag aagccgcgcc cgctagtgat ggactcatcg
     2581 accacgatgg cattttccgg gatcaccatc gcgatcgctt gcgcgatcgc ggcgtaggtc
     2641 agcgcgccgg tcggctttgc aatctcgacc aggggctgcg gcttgacatc tgccggcttg
     2701 gcgcccaccg cgtacgccag cgcctccagc gccgcgaccg aattttctcc gcctgaggtc
     2761 atgcgatgga cctcgcagcc ctccggcttc aacaggctcg gcttgttcgg gtaggcgaag
     2821 aacgccacgg gatcgttggt ttcgaccaga atgatgtggc gaaaatcttt caggatcggc
     2881 agcgcctgct cgatcacata tggaatccgg tcgatcgaga agcgcccgcg cccgcgcgcc
     2941 atccgcggat tgtaggtctg gcccatcacc ttgcagccgg tcttgccggc aatttgcgcc
     3001 gcc
//
LOCUS       DATNOI010000621         7103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_760401,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000621 DATNOI010000000
VERSION     DATNOI010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760401"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VN968_25165"
     CDS             <1..785
                     /locus_tag="VN968_25165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXL32556.1"
                     /translation="VGDIGYFGVTFGMKAKAEGALEPYKPANWDQVAPGLKDPDGYWT
                     TIHSGTLGLFVNKDALGGKAVPACWKDLLKPDYKGMVGYLDPSSAAVGYVGAVAINLS
                     LGGSAANFEPALTFFKELRKNDPIVPKQTSYARVVSGEMPILLDYDFNAYRAKYSEKG
                     NFEFVIPCEGSVVFPYVVGLVKNAPDRDKAKKVIDYLLSDKGQAIWTNAYLRPARQIE
                     LPEAVKAKFLPDSDYARAKSVDWGQMETVQKGFVDRYLAEVR"
     gene            825..1640
                     /locus_tag="VN968_25170"
     CDS             825..1640
                     /locus_tag="VN968_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL32557.1"
                     /translation="MPHKSFVWLCLLPLAVVMTAFFLLPMARLVVTGAEGPQGLAGYL
                     AILIEPRYRATLINTVVLATATTIATLAIATVAGMFLQRHRFPGRAVLIAMLTFPLAF
                     PGVVVGFLIILLAGRQGLIGDLTSRLFGEKLVFAYSIYGLFLGYLYFSIPRVILTIMA
                     AVQKLDVGLEEAARSLGASPWAVQRDVVLPALGPAFVASGAIAFATAMGAFGTAFTLA
                     TNIDVLPMLIYTEFTLAANFSTSAALSVGLGIIAWAILALARSFSGSTVAAAG"
     gene            1645..2430
                     /locus_tag="VN968_25175"
     CDS             1645..2430
                     /locus_tag="VN968_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL32558.1"
                     /translation="MRDRLIFTSQLLFTLLVAAFLVVPAILSISAGVTVNYFRGISSG
                     VTPQWVFQVWDLYAGTILLSFVIAFATLAVTLVVGVPSAYALHVRGGRLSRIVEEIIT
                     LPLAIPGLAIALALLLTYGSLGSFRRSWLFILTGHVIFTMPFMVRSVMAVFATVDIKT
                     LDEGAASLGASPWRRFCDVIVPNAVPGILAGSLMVVTLSLGEFNLTWMLHTPLTKTLP
                     IGLADSYASMRLEVASAYTLIFFVMIIPLLVAMQMFADKEPAR"
     gene            2427..3488
                     /locus_tag="VN968_25180"
     CDS             2427..3488
                     /locus_tag="VN968_25180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32559.1"
                     /translation="MSVAAGHGASVRIEACGKTFADGTRALESATLDIARGETLVLLG
                     PSGCGKTTMLRIIAGLELPDAGGRVLFDDKDMTSVPIEKRNVGMVFQSYALFPNMSVA
                     DNIGYGLKIRGMARQERAARVAELVVLTNITGLENRRVDQLSGGQRQRVALARAVAIR
                     PGILLLDEPLTALDAALRDRLRGELNRLLRALGITTIYVTHDQSEAMELGDRIVVMQK
                     GAIAQIGTPREIYFTPKSRFVAEFIGAANIIEAPVENGHLVLPGGHQPIGGDESVKAA
                     VAMIRPETIRIVEAGGAPLSGIIDSVSFIGDRQRMIVSGASDKLLTVDAPNAVRAQAG
                     ERIGLFIAPDAVRLLPLEN"
     gene            3499..4338
                     /locus_tag="VN968_25185"
     CDS             3499..4338
                     /locus_tag="VN968_25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HXL32560.1"
                     /translation="MLPKPVLVAQISDLHIKSPGQLAYGRVDTAKALERCITALNAFR
                     PRPDLMVISGDLADTPTADEYEHLKRLLSRLDLRLVSIPGNHDSRELMRAAFPHATYA
                     FSSGPLNQSVEIGGLDLVLLDSSVHGKPHGELDAATLQWLEAVLVSSSDRPALLFLHH
                     PPFSTGIWHMDRQNLLNAGELAPMVRRHPRVQLIAAGHVHRATLMIFAGVPATICPAP
                     NHAVDLDLDHLREPSFKVEPPAFHLHSWFPGEGFGSVVTHQVPIGDFDGPHPFFGPDG
                     KLL"
     gene            complement(4491..5219)
                     /locus_tag="VN968_25190"
     CDS             complement(4491..5219)
                     /locus_tag="VN968_25190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXL32561.1"
                     /translation="MRAHQIMTRPVITVTPQTTMVEAANTMLQRHVSGLPVTDAAGKL
                     VGIVSEGDFIRRSEIGTQRRRGRFLKFILGPGQAATDFVHEHGRKVAEIMTPQPLTIT
                     EDTAPEEIVELMEKNNVKRLPVMRGDKIVGIVSRANLLQAVASLAREIPDPTADDDHI
                     RNRVIDTIERNDWCPFGLGVIVRGGVVHLSGVITEERSRKAAVVAAKNVTGVTKVHDH
                     LCWVDTMSGMYLNSPEDDQLAKAG"
     gene            5396..5764
                     /locus_tag="VN968_25195"
     CDS             5396..5764
                     /locus_tag="VN968_25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32562.1"
                     /translation="MTKLSEQSAELVEQSVTDTATRLTKANKNALDFLMSAQKVMLEE
                     LVFTGNEMLERTRTETHLLSEFVSKMAGSHSVKDLKTMYEECGQHQIDFLRRDSERLF
                     KHGERMIEATAKLFGNRPEV"
     gene            complement(5931..6767)
                     /locus_tag="VN968_25200"
     CDS             complement(5931..6767)
                     /locus_tag="VN968_25200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HXL32563.1"
                     /translation="MMACDTTFRIVARRHLEDLTTNHAATSGGDPVALHQMRIALTRL
                     RTAIWFFSPMVVDTEQPRIKDELKWLNTHLGVVRDLDVATEQVQAVDKRRSQAKPDHG
                     AWAVKRDDGHRHLAQALRSARYRRLIKITSDWIESGPWSIRKGKRTTKQRACPIATYG
                     AGKLARWRQKLLKKSRKLPEMDAKQRHRLRLVNKKLCYSIEFLEDLFPGKGFSRQRAA
                     LKYLRKAQKSLGRLNDDANRQSLAAALQPDGGEPLPFLGPKREKRLVRKAAAAYRKLA
                     AL"
     gene            complement(6883..>7103)
                     /locus_tag="VN968_25205"
     CDS             complement(6883..>7103)
                     /locus_tag="VN968_25205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HXL32564.1"
                     /translation="RFVGLFLATDLATRVSRVGRRERDASDATPEIAEIQENYNIGRV
                     DWAVIDASGTSEQTLVQCQARIAHRDPA"
BASE COUNT         1242 a         2162 c         2188 g         1511 t
ORIGIN      
        1 ctgtcggcga tatcggctat ttcggcgtca ccttcggcat gaaggcgaag gcggaaggtg
       61 cgctggagcc gtataagccc gccaactggg atcaggttgc ccccggattg aaggacccgg
      121 acggctattg gaccacgata cattccggca cgctcggtct gttcgtgaac aaggatgcgc
      181 tcggcggtaa agcggtgccg gcctgctgga aggatctgct gaagccggac tacaagggca
      241 tggtcggcta tctcgatccc tcgtcggccg ccgttggata tgtcggggcc gtcgcgatca
      301 atctgtcgct cggcggctcc gcggccaatt tcgagccggc gcttaccttc ttcaaggaac
      361 tgcgcaagaa cgatccgatc gtccccaaac agacatccta tgcccgcgtg gtttccggcg
      421 aaatgccgat cctgctggat tatgatttca acgcctatcg tgcgaaatat tcggagaagg
      481 gcaattttga attcgtgatc ccgtgcgagg gatcggtggt atttccctac gtcgtcggcc
      541 tcgtcaagaa cgcaccggac agggacaagg ccaagaaggt gatcgactat ctgttgtccg
      601 acaaggggca ggcgatctgg accaacgcct atttgcgccc ggcgcgtcaa attgaattgc
      661 ctgaggcggt caaggcgaag ttcttgccgg acagcgacta cgcccgtgca aaaagcgtcg
      721 attggggcca gatggaaact gtgcaaaaag gttttgtcga ccgctatctc gccgaggtcc
      781 gctgatgcag tatgatgcct agatcttgag ggcaccattg cttcatgccg cacaaatcct
      841 tcgtctggct gtgtctgctg ccgctcgccg tggtgatgac ggcattcttt ctgttgccga
      901 tggcgcggct ggtggtgacc ggcgctgaag gtccgcaagg gctggccgga tatctcgcga
      961 tcctgatcga gccacgctac cgcgccacct tgatcaacac cgttgtgctg gcgaccgcga
     1021 ccacgatcgc tacgctggcc atcgcgacgg tcgcggggat gttcctgcaa cgccatcgct
     1081 ttccgggccg ggccgtgctg atcgcgatgc tgacctttcc gctggcgttt cccggcgtgg
     1141 tcgtcggctt cctgattatt ctgctcgcag gccgacaggg tttgatcggt gatctcacga
     1201 gccgcctgtt tggcgagaag ctggtgtttg cctattcgat ctacgggctc ttcctcggct
     1261 atctctattt ctcgatcccg cgcgtcattc tcaccatcat ggcggcggtc caaaaactcg
     1321 acgtcggtct ggaagaggcc gcgcgttcgc ttggcgcaag cccgtgggca gtgcagcgcg
     1381 atgtggtgct gccggcgctg gggccggctt tcgtggcctc tggcgcgatc gcctttgcca
     1441 ccgcgatggg cgcgttcggc acggcgttca cgctggcgac gaatatcgac gtgctgccga
     1501 tgctgatcta taccgagttc acgctggcgg cgaatttttc gacatcggcg gcgttgtcgg
     1561 tcgggctggg aatcatcgcc tgggcgatcc tggctctggc ccgctcgttc agcggaagca
     1621 cggtcgcggc ggccggatga gaccatgcgc gatcgcctga tctttaccag tcagctgctg
     1681 tttacgctgc tggtcgccgc atttctggtc gtgcccgcca tcctgtcgat ttcggccggc
     1741 gtcaccgtga attattttcg ggggatatcg tcgggcgtga cgccgcaatg ggtatttcag
     1801 gtctgggatc tctatgccgg caccatcctg ctgtcgttcg tgatcgcgtt cgcgaccctc
     1861 gccgtcaccc tggtcgtcgg cgttccctcg gcttatgcct tgcatgtccg cggcggcagg
     1921 ctctcgcgga tcgtcgagga aatcatcacc ctgccgctgg cgattcccgg gctcgcgatc
     1981 gccctggcgc tgctgctcac ctatggcagc ctcggcagct tccgtcgttc gtggcttttc
     2041 atcctgaccg ggcatgtcat tttcaccatg ccgttcatgg tgcgatcggt catggcggta
     2101 tttgccaccg tcgacatcaa gacgctcgac gaaggcgcgg cgtcgctcgg cgcatcgcca
     2161 tggcgacgct tctgcgacgt gatcgttccc aatgcggtgc ctgggattct tgcgggcagc
     2221 ctgatggtgg ttacgctttc gctcggcgaa ttcaacctga cctggatgct gcatacgccc
     2281 ctgaccaaaa cgcttccgat cggccttgcc gacagctacg cctcgatgcg gctggaggta
     2341 gcctcggcct ataccctgat tttctttgtc atgatcattc cgcttctggt cgcgatgcag
     2401 atgtttgccg acaaggagcc ggcgagatga gcgtcgccgc ggggcatggc gcgtccgtgc
     2461 gcatcgaggc ttgcggcaag acctttgccg atggcacccg cgcactcgaa tccgccacgc
     2521 tcgatatcgc gcgtggcgaa acgctggtgc tgctcggccc ctccggttgc ggaaaaacca
     2581 ccatgctgcg catcatcgca ggcctcgaat tgcccgatgc cggcggccgg gtactgtttg
     2641 acgataagga tatgacctcg gtaccgatcg agaagcgcaa cgtcggcatg gtgttccagt
     2701 cctacgcgct gtttcccaat atgagcgtcg cggacaacat cggctatggc ctgaagatcc
     2761 ggggcatggc gaggcaggag cgcgccgcgc gcgtcgccga gctggtggtg ctgaccaata
     2821 tcaccgggct cgagaaccgc cgcgtcgacc agctctccgg cggtcagcgc cagcgggttg
     2881 cgctggcacg cgccgtcgcg atccggccgg gcatcctgct gctcgacgag cccttgacgg
     2941 cgctcgatgc cgcacttcgc gaccgcctgc gtggcgagct caaccgtttg ctgcgcgcgc
     3001 tcggcatcac cacgatctac gtgacgcacg atcagtccga ggccatggag ctcggcgacc
     3061 gcatcgtcgt catgcagaaa ggcgcgattg cccagatcgg cacgccgcgc gagatttatt
     3121 tcacgccgaa aagtcgcttc gtggccgaat tcattggcgc tgcaaatatt attgaagccc
     3181 cggtcgaaaa cggtcatctt gtgctgccgg gcggccacca accgattgga ggcgacgaaa
     3241 gcgtcaaggc tgcggtcgcc atgatccgcc ccgaaacgat ccggatcgtt gaggccggag
     3301 gcgcgccgct ctcgggaatc atcgacagcg tcagcttcat cggcgacagg caacgcatga
     3361 tcgtcagtgg cgcgtccgac aagcttctta ccgtcgatgc gccgaacgcg gtgcgagccc
     3421 aggccggcga gcggatcgga ctttttatcg cgccggacgc cgtccgcctg ctgccactgg
     3481 aaaattgaag aagctcaaat gttgccaaaa ccggttctcg tcgcgcagat ttccgatctc
     3541 cacatcaaat cgccgggcca gctggcctac gggcgcgtcg atacggcgaa ggcccttgag
     3601 cgctgcatca ccgccttgaa cgcgttcagg ccaagacccg acctcatggt gatatcgggc
     3661 gatctcgccg atacgccgac ggccgacgaa tacgaacacc tcaagcgcct gctgtcgcgg
     3721 ctcgacctgc ggttggtcag catccccggc aatcacgatt cgcgggaatt gatgcgcgcc
     3781 gcatttccac acgccaccta tgccttttca tccggcccgc tcaaccaaag cgtcgaaatc
     3841 ggcgggctcg atctggtgct gctcgattcc agcgtacatg gcaaaccgca tggcgagctc
     3901 gatgcggcga ccctgcaatg gcttgaagca gtgcttgtct cctcgtcaga ccggcccgca
     3961 ctgctattcc tgcaccatcc gccgttctca accggcatct ggcacatgga ccgacagaac
     4021 ctcctcaatg ccggcgaatt ggcgccgatg gtcagacgcc acccgcgcgt gcaactgatc
     4081 gccgccggtc acgttcatcg cgcgaccctg atgatatttg ccggcgtgcc agcgacgatc
     4141 tgcccggcgc caaatcacgc cgtcgatctg gatctggacc acttgcgcga gccatcgttc
     4201 aaggtcgagc cgccggcgtt tcatctccac agctggtttc cgggggaggg attcggcagc
     4261 gtggtgacgc atcaggtgcc gatcggcgat ttcgacggac cgcacccgtt tttcgggccg
     4321 gacgggaagc tgctgtgagt gagcggaggg ggctcatcca agggctggaa aggctgaaat
     4381 tggctctgct ggagaggtaa tccgaaggtt ggcgcggctc gcctaggcac ggttgaaggg
     4441 cgtgcatctc tcgattattg gagatgcacg ccctctgccg cccccgccga tcagcctgcc
     4501 tttgccaatt ggtcgtcttc cggagaattc agatacattc ccgacatggt gtcgacccaa
     4561 cacaggtggt cgtggacctt ggttacgccg gtgacatttt tcgcggcgac gaccgcggcc
     4621 ttccgcgaac gctcctccgt gatgacgccg ctcaggtgga cgacgccgcc gcgtacgatg
     4681 acgccgagac cgaacgggca ccagtcgttc ctctcgatcg tatcgatgac gcggttgcga
     4741 atgtggtcat cgtcggcagt cggatcggga atttcacggg caaggctcgc taccgcctgc
     4801 aacaggttgg cgcgcgacac gatgccgacg atcttgtctc cgcgcatcac cggcaggcgc
     4861 ttgacgttgt tcttttccat cagttccacg atctcctccg gcgccgtgtc ttcggtgatc
     4921 gtgagcggct gcggcgtcat gatctcggcg actttgcggc cgtgctcgtg aacgaaatcg
     4981 gttgccgcct ggccgggacc gagaatgaat ttcagaaacc ggccgcgcct gcgctgggtg
     5041 ccgatttcgc tgcggcgaat gaaatcgccc tccgagacga tgccgacaag tttgccggcg
     5101 gcgtcggtca ccggtagccc gctgacgtgc cgctgcagca tggtgttcgc ggcttcgacc
     5161 atcgtggtct gcggggtgac ggtgatgacg ggccgcgtca tgatctgatg ggcgcgcatt
     5221 ggcggcctcc gcgggttcga gattagagcc gatatttgac aggctattcc aatcggcgtg
     5281 ccgcgatttg acgcaggtca atgggcggcc gaggcgcgta gacttgatgc agttcaatgc
     5341 gagcgcgggg gcgaagtcct atcatttcaa aaagaacatg aaaggggagg tcgtcatgac
     5401 aaagctttcc gaacagagtg ccgagctcgt cgagcaatcg gtgaccgata ccgcgacacg
     5461 attgaccaag gcgaacaaga acgccctcga tttcctgatg agcgcccaaa aggtcatgct
     5521 tgaggaactc gtattcactg gcaatgagat gctcgagcgg acacgaaccg aaacgcacct
     5581 gttatccgaa ttcgtctcga aaatggctgg atcgcattcg gtcaaggacc tgaagacgat
     5641 gtacgaggaa tgcggccagc atcagatcga ttttctccgc cgcgacagtg agcgcttgtt
     5701 caagcatggt gaacggatga tcgaagccac cgcaaagctg ttcggcaacc ggcctgaggt
     5761 ctgatccatc gtccgactgt catggatttg aagtccaacc atccgccaga cgcgcgatcg
     5821 ggcgagccct gctggctacc gtgtatgggt gttgaacacg cagtcggtca gagtttgccc
     5881 gatgcgccgg gggaccatgt tcgccggtag cgtttggcgg ccccacacac ttagagcgca
     5941 gcgagcttgc ggtatgccgc agccgctttt cgtaccagcc gcttctcacg tttcggaccg
     6001 agaaatggca agggttcccc gccgtccggt tgcagagcgg ccgccaggga ttggcggttg
     6061 gcgtcgtcgt tcaaccgccc gagagatttt tgcgccttac gcagatactt cagcgcggcc
     6121 cgttgtctcg agaatccctt gccgggaaac aagtcctcga ggaattcgat tgaatagcac
     6181 agcttcttgt ttaccaggcg cagccgatgc cgttgctttg cgtccatttc cggaagcttg
     6241 cggctcttct tcaggagttt ttgccgccaa cgcgccagtt tcccggcgcc ataggtcgcg
     6301 atcgggcaag cgcgttgctt tgttgtccgt tttccctttc tgatggacca gggtccactc
     6361 tcgatccaat cggatgtgat cttgatcaga cgtcgatagc gggctgaccg aagcgcttgc
     6421 gccagatgtc gatggccgtc gtcgcgcttc acggcccatg ccccatggtc gggttttgct
     6481 tgcgatcgcc gtttgtcgac ggcctgcacc tgttcggtcg cgacatccag atcgcgaacg
     6541 acgccaagat gggtgttcag ccacttcagt tcgtccttta ttcgtggctg ctcggtatcg
     6601 acgaccattg gtgagaaaaa ccatatggcc gtgcgcaacc gcgtaagcgc gatgcgcatt
     6661 tgatgcaggg ccaccgggtc acccccagat gtcgccgcat gatttgtggt caaatcttcg
     6721 agatggcgtc gcgccacgat ccggaacgtt gtgtcgcacg ccatcattgg attcaaccgc
     6781 gcaatggtgt ggcggcgtgg ccgctgcgtc ggttcgatgc ggaccgcggc tttgcgcttt
     6841 gatttcgggg atggcggcat tgccgtttcg cgcgattggc cttcaggccg gatcacggtg
     6901 ggcgatccgg gcttggcact gtaccagggt ttgctctgaa gttccggatg catcgatgac
     6961 agcccagtca accctgccga tattatagtt ctcctgaatt tcggcaatct ccggcgttgc
     7021 gtcggatgca tcgcgctcgc ggcggccgac gcggctcacc ctggtcgcaa gatcagtcgc
     7081 gagaaagagc ccgacaaacc gga
//
LOCUS       DATNOI010000622         2723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1998505,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000622 DATNOI010000000
VERSION     DATNOI010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998505"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VN968_25210"
     CDS             <1..679
                     /locus_tag="VN968_25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32565.1"
                     /translation="ALKSVDLDINRGEIVALIGANGAGKSTLMMTIFGKPRARSGHIL
                     FDGRDITDVPTHQIARLRIAQAPEGRRIFPRMSVAENLQMGADATECSEAERSAGLER
                     VFTLFPRLKERMTQRGGTLSGGEQQMLAIGRALMSRPRLLMLDEPSLGLAPLIARQIF
                     DAIRTLNRQDGLTVLIVEQNANHALRLAHRGYVMVNGLITLSGTGNELLQRPEVRAAY
                     LEGGRRG"
     gene            936..2054
                     /locus_tag="VN968_25215"
     CDS             936..2054
                     /locus_tag="VN968_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HXL32566.1"
                     /translation="MKSLKLIGLALGASIALSTAALAEDVTIAVAGPLTGGESAFGRQ
                     MKNGADQAVADINAAGGVLGKKLALDAEDDACDPKQARSVAEKIAGAKIPFVAGHFCS
                     SSSIPASEAYADGNVLQITPASTNPLFTERKLWNVARVCGRDDQQGLVAAAYIAKNFK
                     GKNIAILDDKTTYGKGLADETKKALNKAGIKEKLSESYNKGDKDFNAIVSRLKLEKID
                     LVYVGGYHQEAGLILRQMRDQGLKTVLMSGDALADKEYASITGPAGEGTLFTFGPDPR
                     NKPTAKAIVAKFKANNIDPEGYTLYTYAAMQVWSQAVAKAKTTDPKKVMETIKAGAWD
                     TVIGKMEFDAKGDIKQIDYVVYKWDAKGNYTEVNPKGS"
     gene            2196..>2723
                     /locus_tag="VN968_25220"
     CDS             2196..>2723
                     /locus_tag="VN968_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HXL32567.1"
                     /translation="MKNLLTDIAGVRVGHADDARLASGVTAVIFDTPAVAAIDVRGGG
                     PGTREGALLDLANTVERIDAIALGGGSAFGLEAGGGVQAWLAEQGRGFRIREAVIPIV
                     PGAIMFDLLNGGNKAWGRFAPYRDLGYTAAAAASAKFALGSVGAGLGATTANLKGGIG
                     SASAATQSGVCVAALA"
BASE COUNT          529 a          879 c          875 g          440 t
ORIGIN      
        1 ggcgctgaag agcgtcgatc tcgacatcaa ccgtggcgag atcgtcgcct tgatcggcgc
       61 caatggcgcc ggcaagtcga ccctgatgat gacgatcttc ggcaagccgc gcgcccgctc
      121 cggccacatc ctgttcgacg gtcgcgatat caccgacgtg ccgacccatc agatcgcaag
      181 actgcgcatt gcgcaggcgc ccgaaggccg ccggattttt ccgcgcatga gtgtggcgga
      241 aaacctgcag atgggcgccg acgccaccga gtgcagcgag gccgagcgat cggccgggct
      301 cgaacgtgtc ttcacgctgt ttccgcggct caaggagcgc atgacccagc gaggcggcac
      361 cctgtccggc ggcgagcagc agatgctggc gatcggccgc gcgctgatga gccgccctcg
      421 cctgttgatg ctggacgaac cctcgctggg cttggccccg ctgatcgcgc gccagatttt
      481 cgacgcaatt cggaccttga accggcagga cggcctcacc gtgctgatcg tcgaacagaa
      541 cgccaatcac gcgctgcggc tcgcccatcg cgggtatgtc atggtcaacg ggctgatcac
      601 tctgagcgga accggcaacg aactgctgca gcgcccggaa gtccgcgcgg cctatctgga
      661 aggcggaagg cgggggtagc atttcgtgca atactctccg cgggttctct cggcgaacgc
      721 cgggacctcg gtgtcctctc gattttggca cttggcattg agggagatgc gctctgcaac
      781 aattggcgcc aggggccatg ggtcccggcg ttcgctggat ctgccccaaa attgccgatg
      841 acttcgtggc aaaatcagcc gataatggcc gccgcttaga acccggcggc ctgccgggcg
      901 cttcccgtag cgatcaccac ccgcgaggaa ctctcatgaa atcactaaaa ctcatcggcc
      961 tggcactcgg cgcgtcaatc gcgctatcaa cggcagctct tgcggaagac gtgaccatcg
     1021 ccgtggcagg tccgttgacc ggcggcgaat ctgcgttcgg ccggcaaatg aagaacggcg
     1081 ccgatcaggc ggtcgccgac atcaacgcgg ccggcggcgt gctcggcaag aaactggcgc
     1141 tggatgcgga ggacgacgcc tgcgatccca aacaggcgcg ctcggtcgcg gaaaagatcg
     1201 ccggcgccaa aatcccgttc gtggctgggc atttctgctc atcatcctcg attccggcct
     1261 cggaagccta tgccgacggc aacgtgctgc agatcacgcc ggcctcgacc aatccgctgt
     1321 tcacggagcg caagctctgg aacgtggcac gtgtctgcgg ccgcgacgac cagcagggcc
     1381 tggtcgcggc tgcctacatc gcgaagaatt tcaagggcaa gaacatcgcg atcctcgacg
     1441 acaagaccac ctacggcaag ggtctcgcgg acgaaaccaa gaaggcactc aacaaggccg
     1501 gcatcaagga gaagctgagc gaatcctaca acaagggcga taaggacttc aacgccatcg
     1561 tctcgcgcct gaagctcgag aaaatcgatc tggtctatgt cggtggctac catcaggaag
     1621 cgggactgat cctgcgccag atgcgtgatc agggcctcaa gaccgtcctg atgtcaggcg
     1681 atgcgctggc cgacaaggag tacgcctcga tcaccgggcc ggccggcgaa ggcacgctgt
     1741 tcaccttcgg tcccgacccg cgcaacaagc cgaccgccaa ggcgatcgtc gcgaagttca
     1801 aggccaacaa tatcgatccc gaaggctata cgctctatac ctacgccgcg atgcaggtct
     1861 ggtcgcaggc ggtggcgaaa gccaaaacca ccgatcccaa gaaagtcatg gaaacgatca
     1921 aggccggcgc gtgggacacg gtgatcggca agatggagtt cgacgccaag ggcgacatca
     1981 agcagatcga ctatgtcgtc tacaagtggg acgccaaggg caactatacc gaggtcaacc
     2041 cgaaggggtc gtaggacctt acagtttcta ccgcgacatc aagcgccccg gttcgccggg
     2101 gcgtttttgt tgatcgagtg ccagtatgcc cgcgcgcgcc cgacatggtt gaccgcagtg
     2161 cagccgtgcg atcatctacg tccagggagc gcgccatgaa aaacctgctc accgatattg
     2221 ccggcgtccg cgtcggccac gccgatgacg ccaggctcgc gtccggcgtc accgcggtca
     2281 ttttcgacac gccggcggtg gcggcgatcg atgtgcgcgg cggcgggccc ggtacccgcg
     2341 agggcgcgct gctcgatctc gccaatacgg tggagcgaat cgatgccatt gcccttgggg
     2401 gcggctcggc gttcgggctc gaggccggcg gcggggtaca agcctggctc gccgaacagg
     2461 gccgcggttt ccgcatccgc gaagccgtca ttccgatcgt tcccggcgcg atcatgttcg
     2521 acctgctcaa cggcggcaac aaggcctggg gccggttcgc accctaccgc gatctcggct
     2581 acaccgcggc ggcggcggcc agcgccaaat tcgcgctcgg cagcgtcggc gccgggctcg
     2641 gcgcgaccac cgcgaacttg aagggcggga tcggctcggc atcggcggcg acacagagcg
     2701 gcgtctgcgt cgcggcactt gcg
//
LOCUS       DATNOI010000623         4001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2034360,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000623 DATNOI010000000
VERSION     DATNOI010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2034360"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1043..2500)
                     /gene="hydA"
                     /locus_tag="VN968_25225"
     CDS             complement(1043..2500)
                     /gene="hydA"
                     /locus_tag="VN968_25225"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HXL32568.1"
                     /translation="MASLIIRGGTVVNHDHSRRADVLIDDETIVAVGPAIGAPSGATV
                     VDAGGCYVMPGGIDPHTHLELAFMGSVSSDDFEWGTKAALAGGTTMIVDFCIPDPGQS
                     MLAAYQDWRRKSEKAASDYGFHMAVTSWSEQVFDEMAIVVKTYGINTFKHFMAYKGAL
                     MVNDDELYNSFARCAQLGAMPLVHAENGDVVARMQEAVLGRGVTGPEGHAYSRPPEVE
                     GEATNRAIMIADMTGAPLYVVHTSCRESHEAIARARANGKRVYGEPLIQHLLLDATEY
                     ENGNWNHAARRVMSPPFRAKAHQDSLWAGLQSGSLQVVATDHCAFTTEQKRLGIDDFR
                     KIPNGTGGLEDRMPLLWTAAVNTGRLTKEEFVAVTSSNIARILNIYPKKGRLAAGSDA
                     DIVVWDPAASKTISASSQLSRIDYNVFEGYACIGAPIVTLSRGKLAWAMGELRAEKGD
                     GRYVERPPFSPVHVANSTWKQHSAPKAVRRADVTP"
     gene            complement(2658..3440)
                     /locus_tag="VN968_25230"
     CDS             complement(2658..3440)
                     /locus_tag="VN968_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL32569.1"
                     /translation="MQLSVNGSDTFVATGGRDFDAALPTIVLLHGAGFDHSSWALHSR
                     WFAHHGYNVLAPDLPGHGRSSGAALPAISDMADWTAALIEAAGAARARLVGHSMGSLI
                     ALEAAARHPAKVSALSLIGTAATMTVGPDLLKAAEANDRSAIDMVSIWGLGFQAELGG
                     SLAPGLWMHSGAQRVLEQCRPGVLFNDLAACNAYQNALAAAAQVAVPTTFILGERDMM
                     TPAKAGKTLAAVLPNSRTIILRGAGHMMMAERPDELLAALQD"
     gene            complement(3444..>4001)
                     /locus_tag="VN968_25235"
     CDS             complement(3444..>4001)
                     /locus_tag="VN968_25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HXL32570.1"
                     /translation="YHGIVFDEQFGQAAFIMRARTEGLRDFGACLSPTNAFQLLQGVE
                     TLGVRMDRHMANTHAVLEALTANKAVDWVWHPSLESHPDYQLAKRLLPRGAGSIVSFG
                     IKGGRAAGKKFIEALRMISHLANVGDAKTLVIHPASTTHQQMDAAQLKAAGVGEELVR
                     LSVGIESAADIIDDLGQALRISQKA"
BASE COUNT          746 a         1233 c         1273 g          749 t
ORIGIN      
        1 gtgacctcct caggatgagg tctaactcgt tgaaacacaa caacctcatg ctgaggagcg
       61 agcgacgcga gcgtctcgaa gcatgggcag taagcgactc acaaatctaa catagtcagt
      121 actagtcccc gcctagtccc cgccgttttc tttggaaatc cgtcgtcagc cgatcggtgg
      181 aggcgctgac tgcggagcgg atagttgttt ggattcaatc aggatgcgtc ggtacggttc
      241 aaaaaccgcg gcatttcgag gttgctgctt catcgaacaa ttaaaactgc gaacaactaa
      301 acggcctggt ggaaaaattt aacaagacgg gaacattgcc gccgaattgc cacgtacact
      361 tttgacattc accagacatt ttatcggcgc ctgtggctga gcaatacaat ttcgtcgaca
      421 gccgccgcga cccgatgctg aacgaccgat tggacagggg tgtgagatgc gaaggcgaag
      481 actcgaaata tatcgacggg ctgtcgatct ggccaacacc ggcaaatatg acggctggaa
      541 agagatacaa aaggaattgg ccaaggaagg ctacgagcgg actttcggac tgctcgcaag
      601 cgatcgaatc cgctcaacgc tcgatgtcca ttgctcaaag agccggcaag ccatgccgcc
      661 accagcctaa gcatccatgg tttttgattg aatcgttctg tcggcggatc cagttcacct
      721 ctcccgcttg cgggggaggt cgacgcgctc gatagagcgc ggcgggtggg ggaaactgtc
      781 tgccaagcta catccacttc cgatgcggca cccccacccc gagccttcag agcgagcttc
      841 gctcgtctcg cccccgcaag cgggagaggg agctcacttc ggttgttgcc gccgctcgct
      901 tctaatctca tcccgcttta gcatcgttgg atcagcctga ttgttccggc cttgacgccc
      961 gcttcgggct ggcctgcgga gcggtgacga ggtcgccgct ccgcggcttg gctcaagggg
     1021 gttttggccc aagagtcttg gctcaaggcg ttacgtcggc gcgtcgcacg gctttcggcg
     1081 cgctgtgctg cttccaggtc gaatttgcga catggactgg agagaacggc ggacgctcga
     1141 cataacgtcc atcgcccttc tccgcgcgaa gctcgcccat cgcccaggcg agtttgccgc
     1201 gtgacagcgt gacaatcggc gcgccgatgc aggcgtaacc ctcgaacaca ttgtaatcga
     1261 tgcggctcaa ctggctgctg gcgctgatgg ttttgcttgc cgccgggtcc cacaccacaa
     1321 tgtcggcgtc tgagccggcg gcgagccgcc ctttttttgg atagatattg aggatgcggg
     1381 cgatattcga ggaagtcacc gcgacgaact cctcctttgt cagccggccg gtattgaccg
     1441 cagcggtcca gagcagtggc atgcggtctt cgagaccgcc ggtgccgttc gggatcttgc
     1501 gaaaatcgtc gatgccaagt cgcttctgct ccgtggtgaa cgcgcagtgg tcggtcgcga
     1561 ccacctgcag cgagccggat tgcagcccgg cccaaagact gtcctgatgc gccttggcgc
     1621 ggaacggcgg tgacatcacg cgtcgggcgg catggttcca gttgccgttt tcatactcgg
     1681 tcgcgtcgag cagcagatgc tggatcagcg gctcaccata gacgcgcttg ccgttggcgc
     1741 gggcccgggc gatcgcctca tgcgactcgc ggcagctcgt atgcaccaca tagagcggcg
     1801 cacccgtcat atcggcgatc atgatggcgc ggttggtcgc ctcgccttcg acctccggcg
     1861 ggcgggagta ggcgtggccc tcgggaccgg tgacgccgcg ccccagcaca gcctcctgca
     1921 tgcgtgcgac gacgtcgccg ttctcggcgt ggaccagcgg catggcgccg agctgggcgc
     1981 aacgcgcaaa cgagttgtaa agctcgtcgt cattcaccat cagcgcgccc ttgtaggcca
     2041 tgaaatgctt gaaggtgttg atgccgtagg tcttgacgac gatcgccatc tcatcgaaca
     2101 cctgctccga ccacgatgtc acggccatgt gaaagccata gtcgctggcg gctttctcgg
     2161 atttgcgccg ccagtcctga taggccgcaa gcatcgattg cccgggatcc ggaatgcaga
     2221 aatcgacgat catcgtggtg ccgccggcaa gtgcggcctt ggtgccccat tcgaaatcgt
     2281 cggatgacac gctgcccatg aacgccagtt cgagatgggt gtgcggatcg atgccgcccg
     2341 gcatgacgta acagccgccg gcgtcgacga ccgttgcgcc ggagggcgcg ccgatcgcag
     2401 ggccgaccgc gacgatggtc tcgtcgtcga tcaggacgtc agcgcgccgc gaatgatcat
     2461 ggttcacgac ggtaccgccg cggatgatga ggctggccat ggcaactccg aaccggcaat
     2521 tcgaaagatg caactctagc tgtttcggcg gcccgatggt cgctcaaaag tcgggcaacc
     2581 tcgccgcttg tcgcatcgga agccggggcc gatgacgaat ggcgcgccga taatgatggc
     2641 gagggggctc ggcccggtca gtcctgcagc gccgccagca gttcgtcggg acgctcggcc
     2701 atcatcatgt ggccggcgcc gcgcaggatg atggttcgtg aattcggcag caccgcggcc
     2761 agcgtcttgc cggccttggc gggagtcatc atgtcgcgtt cgccgagaat gaaggtggtc
     2821 ggcaccgcga cctgtgctgc ggcagccagc gcgttctgat aggcgttgca ggcggcaagg
     2881 tcgttgaaca gcacgccggg ccggcattgt tcgagaacgc gctgcgcgcc gctgtgcatc
     2941 cacaggcccg gcgcgaggct gccgccgagt tcggcctgaa agccaagccc ccagatcgag
     3001 accatgtcga tcgccgaacg atcgttggcc tccgccgcct tgagcaggtc gggtccgacc
     3061 gtcatggtgg ccgcggttcc gatcaggctg agcgccgaga cttttgccgg atgccgcgcc
     3121 gcggcctcca gcgcgatcaa tgatcccatg gaatggccga caagccgcgc cctggctgca
     3181 ccagcggctt cgatcagcgc cgcggtccag tcagccatgt ccgatatcgc ggggagggca
     3241 gccccggacg agcggccgtg gcccggcaga tccggcgcca gcacattgta gccgtgatgc
     3301 gcaaaccatc ggctatgcag ggcccagctg gaatgatcga agcccgcccc gtgcagcagc
     3361 acgatggtcg gcaaggcagc atcgaaatcc cggccgccgg tggcgacgaa tgtatctgac
     3421 ccgttcacgg acaactgcat cgctcaagcc ttctgcgaga tacggagcgc ctggccgagg
     3481 tcgtcgatga tatcggcggc ggattcgatg ccgaccgaca gccgcaccag ttcctcgccg
     3541 acgcctgcgg ccttcagttg cgcggcatcc atctgctgat gggtggtgct ggcgggatga
     3601 atgaccagcg tcttggcatc gccgacattg gccagatggc tgatcatccg aagcgcttcg
     3661 atgaatttct tgccggcggc ccggccgccc ttgatgccga agctgacgat cgagccggcg
     3721 ccgcgcggca gcaaccgttt ggcgagttga tagtccgggt ggctttccag cgatggatgc
     3781 cacacccagt cgacggcctt gttggcggtc agggcctcca gcaccgcatg ggtattggcc
     3841 atgtgccgat ccatccgaac gcccagggtc tcgacgccct gcagcagttg aaacgcattg
     3901 gtcggcgaca ggcaggcgcc gaaatcgcgc aagccctcgg tgcgggcccg catgatgaag
     3961 gcagcctggc cgaattgctc gtcgaagacg atgccgtgat a
//
LOCUS       DATNOI010000624         2983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1543443,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000624 DATNOI010000000
VERSION     DATNOI010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543443"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VN968_25240"
     CDS             <1..597
                     /locus_tag="VN968_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HXL32571.1"
                     /translation="SNIAECGGFLKTRPDLDVPDIQLHFCMAMVNDHGRKPRWGTGFS
                     CHVCLLRPKSRGSVWLQSADPLAAPAIDPNFFGEDSDVDTMVAGFKMTRRLLDAPALK
                     ALQSSDVFTQGIETDQDIRRVLRERVDTVYHPVGTCKMGVDGFAVVDPKLKVRGVEGL
                     RIVDASVMPTLIGGNTNAPTIMIGEKAADMIKAEMRAN"
     gene            808..1593
                     /locus_tag="VN968_25245"
     CDS             808..1593
                     /locus_tag="VN968_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL32572.1"
                     /translation="MDLGIKGRRALVCASSKGLGRACAMALANEGVHVTLTARGAEAL
                     AKTAAEIRKANPGVTVTEVAGDITTPAGREAALKACPDPDILVNNAGGPPPGDFRNWT
                     RDDWIKAIDANMLTPIELIKATVDGMMARKFGRIVNITSSAVKAPIEVLGLSNGARAG
                     LTGFVAGIARKTVINNVTINALLPGPFDTDRLRGPVAKAEAEKRGITVDQMIAERGKT
                     NPAGRVGDPEEFGLACAFLCGAKAGFITGQNILIDGGAFPGTF"
     gene            1609..2598
                     /locus_tag="VN968_25250"
     CDS             1609..2598
                     /locus_tag="VN968_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HXL32573.1"
                     /translation="MKAVWYERTGPAPDVLTFGEMPTPVAGSGEVRIRLEASGVNPAD
                     AGRRAGSYRPMEFSRVIPNSDGAGIVDQVGDGVTRLQVGRRVWLYNGQRNGRAFGTAA
                     EYITLAEHLVTPLPDDVSFAAGATLGIPCMTAWCCVFGDGAIAGRTVLVTGGAGAVGH
                     YAVQLAKWGGARVIATVSSSAKAEQARLAGADLVIDYKTEDVIARTMAFTEQRGVDRV
                     VDVDFGGNIATTLKLMAMNSTIAVYATNGNRNPVIPMRELMERCIALRPLVLFALPPP
                     LLAAAQVDISKWLSAGKRIHNIAAQFTLSETAQAHLAVERGDKFGTVIVDCVR"
     gene            complement(2602..2982)
                     /locus_tag="VN968_25255"
     CDS             complement(2602..2982)
                     /locus_tag="VN968_25255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HXL32574.1"
                     /translation="MIVKAREFDVKEANSDPDSGSNATDDNMIDVLEDNGSDPVASEI
                     AGFINALSEEEQVDLVALMRLGRGDGTIEEWDDLRREAADERERNRYTARYLLGEPLL
                     GDYLADGLAAFGLSWVDDDEGAPE"
BASE COUNT          551 a          942 c          957 g          533 t
ORIGIN      
        1 tcgaacatcg ccgaatgcgg cggtttcctc aaaacccgcc ccgacctcga cgtgcccgat
       61 atccagttgc atttctgcat ggcgatggtg aacgatcatg gccgcaagcc gcgctggggc
      121 accggcttct cctgccatgt ctgcctgctg cggccaaaga gccgcggcag cgtctggctg
      181 caaagcgccg atccgcttgc cgcgccggca atcgatccga acttcttcgg cgaggattcc
      241 gacgtcgata ccatggtggc tggtttcaag atgaccaggc ggctgcttga cgcacccgcg
      301 ctgaaagcgc tgcagtcctc ggacgtgttc acgcaaggca tcgagaccga ccaggatatt
      361 cgcagggttc tgcgcgagcg cgtcgatacc gtctatcacc cggtcggcac ctgcaagatg
      421 ggcgtcgatg gttttgcggt ggtcgatccg aagctgaaag tccgcggcgt tgaaggcctg
      481 cgcatcgtcg atgcctctgt gatgccgacc ttgatcggcg gcaacaccaa tgcgccgacc
      541 atcatgatcg gcgagaaggc cgccgacatg atcaaggcgg agatgcgggc aaattgattt
      601 catcgccgtc attgcgagga gcaaggcgac gaggcgatcc ggccggtgca gcccggatgg
      661 agcgaagcga aatccggcgc tacgctccat ccgggtacga tgtgctgata tgcatttcag
      721 gcggcgaggg ccacttatca cggccgttga tgttggctac agtaacgaaa ccggacttca
      781 aaatcatctg atagcgggag tgagacagtg gatcttggga tcaaaggccg ccgcgcactg
      841 gtgtgcgcat ccagcaaggg tttgggacgc gcctgcgcga tggcgctggc taacgaaggg
      901 gtgcatgtca cgctgaccgc gcgcggcgcc gaggcgctgg cgaagaccgc ggccgagata
      961 cgcaaagcca atccgggcgt cactgtcacc gaagtcgccg gcgacatcac cacgccggcc
     1021 ggccgcgagg ccgcgttgaa agcctgtccc gacccggata ttttggtcaa taacgccggc
     1081 ggcccaccgc ccggcgattt ccgtaactgg acccgcgacg actggatcaa ggcgatcgac
     1141 gccaacatgc tgacgccgat cgagctgatc aaggccacgg tggacggcat gatggcccga
     1201 aaattcggcc gcatcgtcaa catcacttca tcggcggtga aggcgccgat cgaggtgctt
     1261 ggattgtcca acggcgcgcg cgccggcctc accggctttg tcgcgggcat cgcgcgcaag
     1321 accgtgatca acaacgtcac catcaacgcg ctgctgcccg gacccttcga taccgatcgc
     1381 ctgcgcggcc cggtcgcaaa ggcggaagcg gaaaagcgcg gcattacggt ggatcaaatg
     1441 atcgccgagc ggggcaagac caatcccgcc ggccgcgtcg gcgatcccga ggaatttggc
     1501 cttgcctgcg cgttcctgtg cggagcaaag gcgggcttca tcacgggaca gaacatcctc
     1561 atcgacggcg gcgcattccc gggcacgttc tgatcggagg cgctggcaat gaaggccgtc
     1621 tggtatgagc gaaccggacc ggcgccggat gtgctgacgt tcggcgagat gccgacgccg
     1681 gtggcaggat caggcgaagt ccggatccgg ctggaggcct ccggcgtcaa tcccgccgat
     1741 gccggccgcc gcgccggcag ctatcgcccg atggaatttt cccgcgtgat tcccaatagc
     1801 gacggtgccg gcatcgtcga tcaggtcggt gacggcgtca cgcggcttca ggtcggtcgg
     1861 cgtgtgtggc tctataacgg gcagcgcaac ggccgcgcgt tcggcaccgc agcggaatac
     1921 ataacgcttg ccgagcatct ggtgacgccg ctgcccgacg atgtgtcgtt cgcggctggt
     1981 gccacgctcg gcatcccctg catgaccgcg tggtgctgtg tgttcggcga tggagcgatt
     2041 gcgggccgga cggttctggt caccggcggc gccggcgcgg tcggccatta tgcggtgcaa
     2101 cttgcgaaat ggggcggcgc gcgggtgatc gcaaccgtca gttcgtcggc gaaggcagag
     2161 caggcgcgcc tcgccggcgc cgaccttgtc atcgactaca agaccgagga cgtcatagcc
     2221 aggacgatgg cgttcaccga gcagcgcggc gtcgaccgcg tggtcgatgt cgatttcggt
     2281 ggcaacatcg caacgacctt gaaattgatg gcgatgaatt ccacgattgc cgtctacgcc
     2341 accaacggca atcgcaatcc tgtgattccg atgcgcgaac tgatggaaag atgcatcgcc
     2401 ttgcgcccgc tggtattgtt tgcgctgccg ccacccctgc tcgccgccgc ccaggttgat
     2461 atttcgaaat ggctcagcgc gggcaagcgc attcacaaca tcgctgcaca gtttacgctg
     2521 tcggagaccg cgcaggcaca tcttgcggtg gaaagagggg acaagttcgg caccgtcatc
     2581 gtcgactgtg tgcgctgacc ctcactccgg cgctccctca tcatcatcga cccagctcag
     2641 cccgaacgca gccagtccat ccgccagata gtcgcccagc aggggctctc caagaaggta
     2701 gcgcgcggtg taccggttgc gctcgcgctc atcagccgcc tcgcggcgga gatcgtccca
     2761 ttcttcgatc gtgccgtcgc cgcgcccgag acgcatcagc gcgaccagat cgacctgctc
     2821 ctcttcgctc agcgcattga tgaagccggc gatctcgctc gccaccggat cgctgccatt
     2881 gtcctcgagc acatcgatca tgttgtcgtc ggtggcgttg gacccggaat ccgggtcgga
     2941 gttggcctcc ttgacgtcga attcgcgggc tttcacgatc aaa
//
LOCUS       DATNOI010000625        21113 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_467377,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000625 DATNOI010000000
VERSION     DATNOI010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 21113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21113
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467377"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VN968_25260"
     CDS             <1..1072
                     /locus_tag="VN968_25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HXL32575.1"
                     /translation="SGDAEPQQRFFAAGGFFANDHKARFVAPEVPALRTETTAGRPLR
                     LNTGRVRDQWHTMTRTGISPRLGQHLPEPFVEVHPDDAIRFAVADGDFARVVTDYGQC
                     ILRVVVSERQQRGMLFAPIHWSDANASAARVGALVAPFTDPFSGQPENKATPVSIAPY
                     EYVFRGFALSRAQLELPPHAWWTRVAVTGGYGYLFADNADLAGWQSWLRSVAGSDLAE
                     YKDLGGGIYRAASFAGDHIETCLFVGPARDAGDWDVVKSLFAADALGDDQRRMLLSGK
                     SVDGLASAGPIVCACFGVGRTTILAAIAAGAGSPAEIGVQLKAGTNCGSCIPELKRLI
                     AQTKVAGAEVDPGQRQFARVAN"
     gene            complement(1112..1405)
                     /locus_tag="VN968_25265"
     CDS             complement(1112..1405)
                     /locus_tag="VN968_25265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32576.1"
                     /translation="MSDTEVVTKQLQIAGYSDIKFERIDAEVLVGRDVGEAVAFQLAI
                     GPAGEVYRAAGKEAERQHDVVAAALKKELTKFVRPNGVVMDSSSWMVTARNPG"
     gene            1470..1979
                     /locus_tag="VN968_25270"
     CDS             1470..1979
                     /locus_tag="VN968_25270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32577.1"
                     /translation="MSFDSVSHGLSARVRQTIMVMIPSGFSAARMLRKPATVEADDRT
                     CRSKAMGNFDGGVAPAASDAQDLIAAAKRQRGKHLFAMQTEPSREDVTPRVEFRHENR
                     IPEIDVGTSQFERLRSAYGSASCDLGKSIGCRRAVGALLPSRRLTEVQNPSGVTIGQP
                     GRIIVLFAS"
     gene            complement(2057..2884)
                     /locus_tag="VN968_25275"
     CDS             complement(2057..2884)
                     /locus_tag="VN968_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXL32578.1"
                     /translation="MSPLRRLLGNIGFAFALIVIVSPAVLFFVWMLSLSVKFEIDNAS
                     YPPVFIPERFNWGNYTAVLDSKRFSTYFMNSLIVTGTATAFALLVGVPAGYGLARMRA
                     HKAAVVILIARITPGLSYLIPLFLLFQWLGLLGTLWPQIIIHLVVTVPIVIWIMIGYF
                     ETTPMELEESALIDGATSWQVFRYVALPIARPGIAVAFILAVIFSWNNFVFGIVLAGR
                     ETRTLPVAVYNMISFDQLSWGPLAAAALIVTLPVLLLTVFAQRQIVAGLTAGAVKGG"
     gene            complement(2881..3819)
                     /locus_tag="VN968_25280"
     CDS             complement(2881..3819)
                     /locus_tag="VN968_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004680330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXL32579.1"
                     /translation="MSTIAEPAEVTAGAKGEPKREWRPPSYWPFVLPALIVVLAVIIF
                     PWAYTIWMSLHEWKVGSPTTFVGLANYIRLPSDARFVESVWHTLVYTALSVALPLLFG
                     TLAAVVFHTKFPMRGFLRGLFILPMMATPVAIALVWTMMFHPQLGILNYLLSLIGLPP
                     QLWVFHPATVIPSLVMVETWQWTPLVMLIVLGGLAAIPSEPYESALIDGATRWQIFRY
                     ISLPLIMPFLFIASMIRMIDAVKSFDIIFAITQGGPGSASETINLYLYSVAFVYYDVG
                     YASAIVVVFFALIVALAGALLHLRQRTHWNDAGGVA"
     gene            complement(3816..5141)
                     /locus_tag="VN968_25285"
     CDS             complement(3816..5141)
                     /locus_tag="VN968_25285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HXL32580.1"
                     /translation="MKDTLVSRRAVLAGTAAAGALGLSGFPARAEPQWKKYAGTTIEA
                     NLIKGPRGELLQKYASEFTDLTGIKVESEVIPEQQQRQKAVIELTSGKPSFDVIHVSY
                     HVQKRQFEKAGWLADLSGFMKDPNLTAPDLTVDDFSAGGLKFAQNEKGEMHSLPWSVD
                     YFILYWNKELFAKKGVALPKTFDEMAVAAEKLNDPANGIYGFTGRGLRNANMTLWTNF
                     FLNYGGEFLDDKGNILTDGPEAVEATKMYQRLLTKTAPPGVAGFNWMESMASLTQGRA
                     AMWIDGVGWAPPIEDPNASRVVGKIGYALVPAGPKGQYSAAYGDGVGIAQASTKKEAA
                     YLYCQWAVSKTMGARLLQSGGGVPFRNSVLNDDAVRKGVKMPPEWLDSVIGSAKISKL
                     GLPVIVPVAEFRDIVGAAVTATLSGADPATELKKAHEQFRPILERSEKS"
     gene            5580..6503
                     /locus_tag="VN968_25290"
     CDS             5580..6503
                     /locus_tag="VN968_25290"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXL32581.1"
                     /translation="MKASPFGPDDQTGATNRITPAVTKAAAAEIQTGVVIPMAFNLVD
                     GVPLFGTRFTKTILTTASLVPGGEYGKNRLTYMEDTYLSQSHVGTHIDGMGHIGIMDC
                     YYNQTPMGKFVTQNYLKKLGLENLKTFATRGVIIDMVKVFQDAGKLKTNPACRTPCLD
                     GGTVITEADIQAGLKMYNVALREGDVVFLHTGWGDLFTQFPAQNATYNKAAPGIGKSA
                     AQWLADQKVVAVGTDTWVVEVIPGEDTNEAFPVHALLLTDNGIHIIENVRTDLIAAEA
                     ASSKRATFFLSMTVPKAVGTTGTFVNIEAIR"
     gene            6553..7551
                     /locus_tag="VN968_25295"
     CDS             6553..7551
                     /locus_tag="VN968_25295"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXL32582.1"
                     /translation="MKRRHFLLAILAVASSTRAANGEARRFRVAFANLNEEPGTSIEG
                     LGFSGAEIRRSFELASRGLPLDVIYYDNGGDAGKAVANAADAVSRKVDLLIEYNSDAE
                     ANVEIGRQLKAAGIPVLALNQPIPGAPLYAADHLAAGRIAGQTLGQFARENWPDQTPV
                     AAILGDVADPSPSVAARIQGVTEGLRQELPNVPPILLDSAGHSVRAEGVFAKFLGTQT
                     RRKLLVATLDDASALAAKSAIETAGRTSDCVIVSQGASRKVHGGASEKKEIDPANRGS
                     IVLGSVAYFLDRYGYEVLPIAMRMLRGEQVPAQTLTNHVLISAKNVFREYPPYDMN"
     gene            complement(7570..8913)
                     /locus_tag="VN968_25300"
     CDS             complement(7570..8913)
                     /locus_tag="VN968_25300"
                     /inference="COORDINATES: protein motif:HMM:TIGR05002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat
                     NxxGxxAF-containing protein"
                     /protein_id="HXL32583.1"
                     /translation="MDASGMPPYGGPLLASARSRLTIAGAAVLLLWIAVLWASLSEPA
                     RKAALQNAAPEAPALRLVVASGQAAPTGGRFDRFDVGSQPIVAPVNGRGQVAFYASVA
                     RAKATEGIFLATASRITKAAAVGDAVPGGGVLSEFARHPVPALNDAGKIAFGANVAGA
                     RATEGIFLAADDRLKAIALSGTDAPGVQNGTFVEFDAPVLNNRDEIAFVGTVRRGRET
                     LQVLYLYSGGVLRKLVAGGDPAPRGGSFDRFGLPVINNKGVVAFPAILERGAVLGGIF
                     VAGARELRLLVGAGEIGPEGAMLVRFSERASIDDEDNVAFVAHLGSGTSSEAVLVASA
                     SGLTQLAIVGESAPGGGRFAAFGPWPNFGPNATVAFVAAIDAGPGTLGIYLGTPGAIH
                     RVAMVGDHLANGDVIPALALNSVTSVGSRGGLTFATMANPESGQNGIYYYGPPGS"
     gene            complement(8918..9853)
                     /locus_tag="VN968_25305"
     CDS             complement(8918..9853)
                     /locus_tag="VN968_25305"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HXL32584.1"
                     /translation="MKRDASISTPFRFVFVCVGILLGIGPAAAADKIKVVATFSVIGD
                     MVANVAGDRVDLVTLVGPGGDTELYKPSLADGKSVAEAKIVFMNDLNDEFEPWLEPLL
                     KGVKFGGTKIVVSRGAKTYSSSEERAPRSKAAEEIDQHAWLDPKNGVIYVRNIAAALT
                     RSDPANAPDYRARAAAYIKQLQDLDAWAKTEMAAVPPAKRRILSSHDSLQYLARAYGI
                     TMLAINGWTNNSEPSAAELAGLTQKIRQEGIHALFLDSITDPRAMQQVAQETGATIGG
                     TLYGDALSKPGEEADTYLKMVRHDVTTLIAGMMKN"
     gene            complement(9850..10665)
                     /locus_tag="VN968_25310"
     CDS             complement(9850..10665)
                     /locus_tag="VN968_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HXL32585.1"
                     /translation="MRIALVASFALALANGPIGTLLLMRRESLSGNVLSHAVMPGAAL
                     GFLYAGYSLAALSFGGLLTGLVVAGLAALMARSKSQRQDVSLIAFYLVSLSFGILIVA
                     ARGSQADLMHVLFGTVLAVDVNALLLIACITSITLLIIAALYRPLAVESFDPAFLRAA
                     GGGGGLFRAIFITLVVLNLVASFQAFGTLLAIGPMLLPAAAARCWTQKVWPMIALATV
                     IGMAAALAGILISYYANLPSGPAIVMGAGLIYGISLLAAGRTRIKPRGSGEQR"
     gene            complement(10716..11591)
                     /locus_tag="VN968_25315"
     CDS             complement(10716..11591)
                     /locus_tag="VN968_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HXL32586.1"
                     /translation="MGGAAIAPLRRGLAVSLSALSDHAQAAFTRHFDGGYDPSPLDGP
                     GQRGDGAIAVRNLSVGYGEHLALEGVTGDFAPASMTAVVGPNGAGKSTLLKVLAGIIR
                     PMAGEVTCAALARHRLAYLPQQAELDRGFPITVGELVALGDWRNFGSVREPLPRLATR
                     VHEAVATVGLEAFINRQIAELSDGQFQRALFARLLLQDADVILLDEPFAAVDESTTDE
                     LLNLLQRWREKGQTIVAVLHDLDRVRLHFPSTLLLARSLIAWGDTSLSLSADNLARAH
                     VKPGLSHGGRFGRAA"
     gene            11885..12801
                     /locus_tag="VN968_25320"
                     /pseudo
     CDS             11885..12801
                     /locus_tag="VN968_25320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
     gene            complement(12809..13675)
                     /locus_tag="VN968_25325"
     CDS             complement(12809..13675)
                     /locus_tag="VN968_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL32587.1"
                     /translation="MPRRIDHLIICVRDLAQAALSWRALGFTLTPVGVHPFGTSNRLA
                     MFANSFLELLAVTDAAAIPAAAPCRFSFAAHNRDFLAAAEGMSMLAWHSTDAHADAAR
                     FKAKGIGAYAPFDFGRDAVLPDGATARVGFSLAFATHPAMPGIAFFTCQQRHPPELFW
                     KPDYQRHPNGALRVIEVVMSAPEPAAHQNFLERITESSAELAPGRLTVEEAGDRITVL
                     SPLEIARQLPGLAADGPPRFCAARLAVADLDATRRELQRNAVDFQINGGVLLIPPAAC
                     HGLALEFVEQPA"
     gene            13805..14170
                     /locus_tag="VN968_25330"
                     /pseudo
     CDS             13805..14170
                     /locus_tag="VN968_25330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603589.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14330..15169
                     /locus_tag="VN968_25335"
     CDS             14330..15169
                     /locus_tag="VN968_25335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXL32588.1"
                     /translation="MKSRPFGNSKCEVSVIGQGTWYIDRGDRRSAVAALRRGVDLGMT
                     HIDTAEMYGDAEPVIAEAIAERREEIFLVSKVLPSNASRRGTITACERSLARLKTDRL
                     DCYLLHWRGSYSLDETVAAFDHLVDAGKIRSWGVSNFDTDDLDELLAVAGDGKIACNQ
                     VLYHLGERAIEHAVIPWCEQHGVAVVAYSPFGHNDFPPARSKAGQALQAIAKAHGASP
                     RQVALSFLTRATSVFAIPKASSQEHAADNAAAANLTLSEDEITALDKAFPRGPKPRGL
                     PML"
     gene            15327..16313
                     /locus_tag="VN968_25340"
     CDS             15327..16313
                     /locus_tag="VN968_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXL32589.1"
                     /translation="MTPPPAAAARLDSNALSLPEKKLSERTAPARADRPFRGIALILA
                     STVFLGASDATAKYLSATLPSIEIAWIRFLVFGMIMFPAMLPGSPLYALPSGRRGLQL
                     MRGAALLASSLFFITGLGFLPIAEASATGFVSPLFVTALSIVFLGESVGVRRWLATAV
                     GLIGVLIILRPGSSAFHPAAFFPIVSALAWACTMIMTRMMSGKEPVITTMTYSSIAGV
                     CILSALVPFVWVAPSWHDVLFGIFIGIASTAGQWIVVLAFRYADASVLAPFQYTQLLW
                     VSILGFMIFGELPDIWTVTGAVFIVASGLYTAHRERIRRSQLPMQAEPSPNA"
     gene            16298..16495
                     /locus_tag="VN968_25345"
                     /pseudo
     CDS             16298..16495
                     /locus_tag="VN968_25345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            16606..17688
                     /locus_tag="VN968_25350"
     CDS             16606..17688
                     /locus_tag="VN968_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXL32590.1"
                     /translation="MRAAIFRNGEIVVDTLPEPKPAAGQVLVKTLACGICGSDLHARK
                     HAHHMVELARHLPGRKSMDLTRDVVFGHEFCCEILDYGPATSRKLKAGTRVCSLPALL
                     TSDGPQGIGYSNDNIGGYAERMLLSEALLLEVPNGLAAEHAALTEPLAVGVHAVAKAN
                     VRGDEVPLVIGCGPVGLAVIAALKLKGLHPIVAADYSPARRALAEKLGADIVVDPARN
                     EPYATWAEHAAMSPEQKAARPPLHAWRPALKPALIFECVGIPGLLQQVFEGAPRDARI
                     VVVGVCMETDRSEPTLGIMKELNVQYVLGYTPDEFASSLRLIAEGQVDAASLVTATVG
                     IDGVATAFADLANPERHTKIIVEPWR"
     gene            17856..18101
                     /locus_tag="VN968_25355"
                     /pseudo
     CDS             17856..18101
                     /locus_tag="VN968_25355"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            18205..19317
                     /locus_tag="VN968_25360"
     CDS             18205..19317
                     /locus_tag="VN968_25360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32591.1"
                     /translation="MSASMLRVVVVKVAASALLAAIFAACVSIWPLSLMRKAVDDLEA
                     ARLEKYDADLARVPANAPLWEWTPFLETQDGTKQAKALDSIRSIDQRQAQAEAMLDRG
                     DFPIGYLGSFDLDPTPALCEKARSLLRRRVEPLVLKTPDPKPYSDIALPLADAISGMN
                     WLVGYDCACDAEAAAWEAMAKGYSNTSLDVHHLAELRDPKELGRTLRERPERFSMLNA
                     KSHLKGWLRFADDDSLHDRALTGARALDHRTTDAVEILIDKYDEESRWKLLRYLTALD
                     LDATMPLCANLLSELHGQFTKVYRPGANDEPRPYSELLERLGNGEQLPDLIWLAGHGC
                     AAEPELSEAVALVNAYRDSPGRAEMLTTLTRLRGRR"
     gene            complement(19325..19786)
                     /locus_tag="VN968_25365"
     CDS             complement(19325..19786)
                     /locus_tag="VN968_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXL32592.1"
                     /translation="MHTLDAIDRKILSQLQSDSRTTMQELADKVGLSVSPCHRRVKLL
                     EARGVITRYIATVDQKSLGLHVSVFISIKLARQKEEDLNRFAKAISKWDEVLECYLMT
                     GNRDYLLRVVAADLSSYEAFLKNKLTRLDGIASIESSFALSQVKYSIALPV"
     gene            19925..>21113
                     /locus_tag="VN968_25370"
     CDS             19925..>21113
                     /locus_tag="VN968_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXL32593.1"
                     /translation="MSIEPARLELLSALARKVLWLSSWTIHHANHVRPNVDGLKVGGH
                     QASSASLATIMSALYFAALRPEDRVAVKPHASPVFHAIQYLFGHQTRDKLENFRGFKG
                     AQSYPSRTKDTDDVDFSTGSVGLGVAQTLFSSLVQDYVKAHGWMKRRPEGRMIALVGD
                     AEMDEGNIFEALLEGWKHGLRNTWWVVDYNRQSLDAVVREGLWEKFESMFRNFGWDVV
                     IVKYGKLMQAAFAEPGGEALKRWIDDCPNAMYAALCFQGGAAFRKHLTDEIGDQGAVS
                     RLIDKRSDDELLALMSNLGGHDMASMVEAFEAIDHDRPVCFIAYTIKGVGLPFQGHKD
                     NHAGLMTVAQMEKWRGAQNIRPGHEWEKFEGLSQDPAALEAFLGQAPFNRDGRRRLTA
                     DVIE"
BASE COUNT         3977 a         6512 c         6705 g         3919 t
ORIGIN      
        1 ctccggcgat gccgagccgc agcagcggtt tttcgcggca ggcggatttt ttgccaacga
       61 ccacaaggca cgctttgtcg cgccggaagt tcccgcgctg cgcaccgaaa ccacggccgg
      121 gcggccgttg cggctgaaca ccggccgcgt tcgggaccaa tggcacacca tgacccgcac
      181 cggcatcagc ccgcggctcg gccagcatct gccggagccg ttcgtcgagg ttcatcctga
      241 cgacgccata aggttcgctg tcgccgatgg tgatttcgcg cgcgtcgtca cagactacgg
      301 acaatgcatt ctcagggtcg tggtgagcga gcggcagcag cgcggcatgc tgttcgcgcc
      361 gatccactgg agcgatgcca atgcgtccgc cgcgcgcgtc ggcgcgttgg tggcgccgtt
      421 caccgatccg ttctccgggc agcccgagaa caaggcgacg ccggtatcga tcgcgcccta
      481 tgaatatgtt tttcgcggtt ttgcgttgtc gcgcgcacag ctcgaattgc cgccccatgc
      541 gtggtggacg cgggtggccg tcaccggcgg ctacggctat ctgtttgccg acaatgccga
      601 cctggcggga tggcaatcct ggctgcggtc agtcgccggc agcgatctcg ccgaatacaa
      661 ggatttgggc ggcggcatct atcgcgcggc atcgttcgcg ggagatcata tcgaaacatg
      721 cctgttcgtc ggcccggcgc gggatgcggg cgattgggat gtggtcaaga gcttgttcgc
      781 cgctgatgca ctcggcgacg atcagcgccg gatgttgctg tcgggcaaat cggtcgacgg
      841 cctggcgagc gccggcccga tcgtctgcgc ctgctttggc gtcggccgca ccacgattct
      901 cgcggccatc gcggccggcg ccggatcgcc cgccgagatc ggcgtgcaac tgaaagccgg
      961 caccaattgc ggctcgtgca ttccggaatt gaagcgattg atcgcgcaga cgaaggttgc
     1021 tggcgccgaa gtcgatcccg gacagcgtca gttcgcccgc gtggcgaact gagggaattc
     1081 tagccgcgtg cggcggcttg cgtcgcaacg attaccccgg gtttctcgcc gtcaccatcc
     1141 aggagcttga atccatcacc acgccgttcg ggcggacaaa tttcgtcaat tccttcttca
     1201 aggcagcggc caccacgtcg tgctgacgtt ccgcctcttt gccggctgcg cgataaacct
     1261 cgccagccgg tccgatcgcg agctgaaacg cgacggcctc accaacgtct cgcccgacca
     1321 aaacctcggc gtcaatccgt tcgaatttga tgtcggagta tcccgcgatc tgcaattgct
     1381 tggttacaac ctcggtatcg ctcatggaaa atggtccagg accgcaagtg gcggcatctt
     1441 cgccgaccgg cggcaagtag cgcagcacaa tgtcttttga cagtgtgagc catgggttgt
     1501 cggcccgcgt ccgccaaaca atcatggtta tgattccgtc cggcttcagc gccgcgcgca
     1561 tgttgcgtaa acccgcgaca gtcgaggccg acgacagaac ctgtcggtcc aaggcgatgg
     1621 gcaattttga tggtggtgtc gccccagccg catccgacgc ccaggacctg atcgctgctg
     1681 cgaagcgcca gcgcgggaaa caccttttcg ctatgcagac tgagccctcc agagaggatg
     1741 tgacgccacg tgttgaattt cggcacgaga accgtattcc agaaatcgac gtagggactt
     1801 cgcaattcga gcgcctgcgc agtgcttatg gttcggcatc ctgtgatctt gggaaatcta
     1861 tcggttgtcg tcgggccgtc ggcgcgttgc tgccgtcgcg gcgtctaacg gaagttcaaa
     1921 atccatccgg cgtcactata ggccagcccg gccggatcat tgtcctgttt gcgtcataac
     1981 tatccgtgga atgaatcttt cgtcgtgatt tcgggtaaaa attctgttgg ctcgcgtagc
     2041 gagctggggc ggaactctac ccgcccttga ccgcgcccgc cgtcaacccc gccacgatct
     2101 ggcgctgcgc gaataccgtc aatagcagca ccggcagggt gacgatcagc gccgcggcgg
     2161 cgagcggtcc ccagctcagc tggtcgaagg aaatcatgtt gtagacggcg acgggcaggg
     2221 tacgggtttc gcgcccggcg agcacgatgc cgaagacaaa gttgttccag gagaagatca
     2281 ccgccaggat gaacgcaacc gcgatgcccg gccgtgcgat cggcagggcg acgtagcgaa
     2341 acacctgcca gcttgtggcg ccgtcaatca gcgccgactc ttccagctcc atcggggtgg
     2401 tctcgaaata gccgatcatg atccagatca cgatcggcac ggtcaccacg aggtggatga
     2461 tgatctgcgg ccagagcgtg ccgagcaggc cgagccactg gaacaacagg aacagcggaa
     2521 tgagatagga caggcccggt gtgatgcgcg cgatcaggat caccaccgcg gccttgtgcg
     2581 cccgcatgcg ggcgaggcca tagcccgcgg gcacgccgac caatagtgcg aacgccgtcg
     2641 cagtgcccgt cacgatcagg ctgttcatga agtaggtcga gaacctcttg gagtcgagaa
     2701 ccgcggtata gttgccccag ttgaaacgct cgggaatgaa aaccggtgga tacgacgcgt
     2761 tgtcgatctc gaatttgacg gataacgaca gcatccagac gaagaacagc acggccggcg
     2821 acacgatgac gatgagcgcg aaggcaaagc cgatgttgcc gagcaagcgt cgcagcgggc
     2881 tcatgccacg cctccggcgt cgttccagtg cgtcctttgc cgcaggtgaa gcagcgcacc
     2941 cgccaaggcg acgatcaggg cgaagaacac gaccacgatc gccgaggcat aaccgacatc
     3001 gtaatagacg aaggcgacgc tgtagagata gaggttgatc gtctccgagg ctgagccagg
     3061 gccgccttgc gtgatcgcga agatgatatc aaaacttttc accgcgtcga tcatgcggat
     3121 catcgatgcg atgaacagga atggcatgat cagcggcagc gagatgtagc ggaaaatttg
     3181 ccagcgggtg gcgccgtcga tcagcgcgct ctcgtagggc tcgctcggga tcgcggcgag
     3241 gccgccgagc acgatcagca tcaccagcgg tgtccattgc caggtttcaa ccatcaccag
     3301 cgacgggatc acggtcgcgg gatgaaacac ccaaagctgc ggcggcaggc cgatcagcga
     3361 caagaggtag ttgaggatgc cgagctgggg gtggaacatc atggtccaca ccagcgcgat
     3421 ggcgaccggg gtcgccatca tcgggagaat aaacaggccg cgcaaaaatc cgcgcatcgg
     3481 gaatttggtg tgaaacacca ccgccgccag cgtgccgaac agcagcggca gcgcgaccga
     3541 cagagcggta tagaccagcg tgtgccacac cgattcgacg aagcgcgcat cgcttggcag
     3601 gcggatgtaa ttcgccagcc cgacaaaggt ggtgggcgag ccgaccttcc attcgtgcag
     3661 gctcatccag atcgtgtagg cccacggaaa gataatgacc gcgagaacca cgatcagcgc
     3721 cggcagcacg aacggccagt aggatggcgg ccgccactcc cgcttcggct cgcccttggc
     3781 gccggctgtg acctcagccg gctcggcaat cgtgctcacg acttttcgct gcgctccaga
     3841 atgggacgga attgctcgtg cgcctttttc agttcggtcg cagggtccgc cccggacagc
     3901 gtcgcggtga cggcggcgcc gacgatgtcg cggaactcgg cgaccggaac gatcacgggc
     3961 aggccgagtt tcgagatttt ggccgagccg atcaccgaat ccagccactc cggcggcatt
     4021 tttacgccct tgcgcacggc atcgtcgttg agcacggagt tgcggaacgg gacgccgccg
     4081 ccgctctgca agagccgcgc gcccatggtc ttcgacaccg cccattggca atagagatag
     4141 gcggcttcct tcttcgtgct cgcctgggca atgccgacgc cgtcgccata ggcggcggaa
     4201 tactgacctt tgggtccggc cggcaccaag gcatagccga tcttgccgac cacacgcgag
     4261 gcgtttggat cttcaatggg tggcgcccag ccgacgccgt cgatccacat cgctgcacgg
     4321 ccttgcgtca gcgaggccat cgactccatc cagttgaaac cggcgacgcc cggaggcgcc
     4381 gtcttggtca gcaggcgctg atacattttg gtggcttcga ccgcctcggg cccgtcggtc
     4441 aggatgttgc ccttgtcgtc gagaaactcg ccgccgtagt tgaggaagaa attggtccac
     4501 agcgtcatgt tggcattgcg caggccgcgg ccggtaaagc catagatgcc gttggcggga
     4561 tcgttgagct tctcggcggc gacggccatc tcgtcgaagg ttttgggcaa tgcgacgccc
     4621 ttcttcgcga acagctcctt gttccagtaa aggataaaat agtcgaccga ccagggcagc
     4681 gaatgcatct ctcccttctc gttctgggcg aatttcaggc cgccggcgga aaagtcgtcg
     4741 accgtgaggt cgggcgccgt caggttcggg tctttcatga aaccggagag gtccgcgagc
     4801 cagccggcct tttcgaattg ccgcttctgc acgtgatagc tgacgtgaat gacgtcaaaa
     4861 ctcggcttgc cggaagtgag ttcgatgacg gccttctgcc gctgctgctg ctccggaatg
     4921 acttcggatt caaccttgat accggtcagg tcggtaaact cggacgcgta tttctgcagc
     4981 aactcgcctc ttggtccctt gatcaggttg gcctcgatcg tggtgccggc atacttcttc
     5041 cactgcggct cggcgcgcgc gggaaatccg gaaaggccga gcgctcccgc cgcggcggtg
     5101 ccggccagga cggcacggcg agaaacaagg gtatccttca ttggtattct ccctggaggt
     5161 cggcttattt tgacggcatg ttagcgcctg cgcggtttcg gcaacagccc gatcgtgatg
     5221 tatgcggttg ctttgtttgg ttttctccaa ggcgccttgc atccgcagca acacctcaaa
     5281 ccgcattgca caaagtgccg ctgaatcggc cagatgaccg cgcgattgca gcctcatgaa
     5341 aatggttccg cagagccgct caaatacgac caaaattgga gaagacccaa gacgccgatt
     5401 gaaacggctt gcgaggcctg ccatgattgc tgaccataat gtgcagactc aactgcaccg
     5461 gcagatccag ggaggcgatc atgaaataca ctgcgatcgt tgcaagtgtc atcttatcgg
     5521 cgttagcgct cttgggtagt tcgaacgtca ctctcgcgca gagtgattgc aagactttgg
     5581 tgaaggcatc tcctttcggt ccggacgatc aaaccggcgc gaccaaccgc atcacccccg
     5641 ccgtgaccaa ggccgccgcc gcggaaatcc agaccggcgt tgtcattccc atggccttca
     5701 atctggtcga tggcgtaccg ctgttcggta cccggttcac caagacgatc ctgacgaccg
     5761 cctcgctcgt tcccggtggc gaatacggca agaaccgatt gacctacatg gaagatacct
     5821 atctcagcca aagccatgtg gggacgcata tcgacggaat gggtcacatc gggatcatgg
     5881 attgctatta caatcagacg ccgatgggaa agttcgtcac ccagaactac ctgaaaaagc
     5941 tggggcttga aaacctcaag actttcgcca cccgtggcgt gatcattgac atggtcaagg
     6001 tgtttcagga cgccggcaag ctgaagacca atccagcctg ccggacgccg tgtctcgacg
     6061 gtggcaccgt gatcacggaa gccgacatcc aggctggtct gaaaatgtac aatgtcgcct
     6121 tgcgcgaagg cgatgtggtg ttcctgcata ccggatgggg tgacctgttc acccagttcc
     6181 cggcgcagaa cgccacctac aacaaggcgg cgcctggcat cggcaaaagt gcggcgcaat
     6241 ggctcgccga tcaaaaggtg gtggcggtcg ggaccgacac ctgggtggtc gaggtgatcc
     6301 ccggcgagga taccaacgaa gccttccccg tccacgcgct gttgctgacg gataacggca
     6361 tccacatcat cgagaacgtt cgtaccgatt tgatcgccgc tgaggccgcg agcagcaaac
     6421 gcgcgacctt cttcctctcg atgacggtcc cgaaggcggt gggcaccacc ggcaccttcg
     6481 tcaatatcga agccatcagg tagtgtgtct gcggcggatc ttgcagaacc ccacgttgtg
     6541 ccggcgcttc acatgaagcg ccggcatttc ctgctggcga tcctcgccgt cgcctcgtcc
     6601 acgcgcgcgg ccaacgggga agcgcggcgc tttcgggtgg ctttcgccaa tctgaacgag
     6661 gagccgggca cgtcgatcga gggcctgggc tttagcgggg cggaaatccg ccgcagcttc
     6721 gagctggcgt cgcgcggatt gccgctggac gtgatttatt acgacaatgg cggcgacgcc
     6781 gggaaggccg ttgccaacgc ggcggatgcc gtttcccgca aggtcgactt gctgatcgaa
     6841 tataattccg acgcggaagc caatgtcgaa atcggccgcc agctgaaagc agcgggcatt
     6901 cccgtactcg cgctgaacca acccattccg ggcgcgccgc tttatgcggc cgaccatctc
     6961 gcggcgggca ggatcgccgg ccaaaccctc ggacaattcg cgagggagaa ctggcccgac
     7021 cagacgccgg tcgccgcgat cctcggagac gtcgccgacc ccagcccgtc cgtcgccgcg
     7081 cgcatccagg gagtgacgga agggttgcgg caggaactgc ccaatgttcc gccgatattg
     7141 ttggacagcg ccggccattc agttcgcgcc gagggcgtct tcgccaagtt tctcggcact
     7201 cagaccaggc gcaaactgct tgtcgcgacg ctcgacgatg cctccgccct cgctgccaaa
     7261 tcggcgattg aaaccgccgg gcgcaccagt gattgcgtca ttgtgagtca aggcgccagc
     7321 cgcaaagtcc atggcggtgc cagcgagaag aaggagattg atccggccaa tcgcggcagt
     7381 atcgttctgg gttcggtggc ctatttcctt gaccgctatg gctatgaagt tctgcccatc
     7441 gcaatgcgga tgcttcgggg cgagcaggtg ccggcgcaaa ctctgaccaa ccacgtcctg
     7501 atttccgcaa agaacgtatt tcgcgagtat ccgccgtatg acatgaactg agctcagttc
     7561 catataagct cagcttcccg gcggcccgta gtaatagatg ccgttctgac cgctttccgg
     7621 attggccatg gtggcgaatg tcaggccgcc gcgcgaaccg accgaggtga cggagttgag
     7681 tgcgagagcg gggatgacgt cgccattggc caagtggtcg ccgaccatgg cgacccgatg
     7741 aatggctccg ggggttccga gataaatccc cagcgtcccc ggcccagcat cgatggccgc
     7801 gacgaaggcg actgtggcgt tggggccgaa attaggccat ggcccgaaag cggcgaagcg
     7861 gccgccgccc ggcgcgctct cccccacgat cgccaactgc gttaaaccgc tggcacttgc
     7921 gaccagcacg gcttcggaag aggtgcccga gccgagatgc gcgacgaagg cgacattatc
     7981 ctcgtcatcg atcgaggccc gctccgaaaa gcgcaccagc atcgcgcctt ccggtccgat
     8041 ctcgccagcg ccgaccagaa ggcgcaattc gcgggcgccg gcgacgaaga ttccgcccag
     8101 caccgcgccc cgctcaagga tcgcggggaa ggcgaccacg cccttgttgt tgatgaccgg
     8161 aagaccgaag cggtcgaagc tgccgccgcg tggggctgga tcaccgcccg ccaccagctt
     8221 gcgcaacacg ccgccactgt aaagataaag cacctgcagc gtttcccggc ctcgccggac
     8281 cgttccgaca aaagcgattt catcccgatt gttcagcacc ggcgcatcga attcgacgaa
     8341 ggtgccgttt tgcacgccgg gtgcgtcggt cccgctcagc gcgatagctt tgaggcggtc
     8401 gtcggcggcg agaaaaatcc cctcggtggc gcgcgctccg gcaacgttcg cgccaaatgc
     8461 gatcttgccc gcgtcgttca gcgccggaac gggatgccgc gcaaattcag acagcacgcc
     8521 gcccccgggt accgcatcgc cgaccgccgc cgctttcgta atccgtgagg cggtggcaag
     8581 aaaaatacct tcggtcgcct tggcccgcgc cacgctggcg taaaaggcga cctgccctcg
     8641 accgttgacc ggcgccacga tcggctgcga gccgacgtcg aatcggtcga aacgaccgcc
     8701 tgttggtgcg gcctgtccgg aggcgacgac gaggcgcagg gcaggtgcct ccggagccgc
     8761 attctgcaaa gccgccttgc gggctggttc cgacaaactg gcccacaaca cggcgatcca
     8821 cagcagcaac accgccgcac ctgcgatcgt caggcgggaa cgcgcgctcg ccaggagtgg
     8881 tccgccatac ggcggcatgc cggaggcatc cattttgtca atttttcatc atgccggcga
     8941 taagcgtcgt cacgtcgtga cgaaccattt tgaggtaagt gtcggcctct tcgcctggct
     9001 tcgacagcgc gtcgccataa agcgtaccgc cgatggtagc accggtctcc tgggcgacct
     9061 gttgcatggc acgcggatcg gtgatgctgt cgaggaacag cgcgtgtatg ccctcctgtc
     9121 ggatcttttg ggtaagacca gccagctcag ccgcggacgg ctcgctgttg ttggtccagc
     9181 cattgatcgc gagcatcgta ataccgtagg ccctggcaag atattgcaac gaatcgtgcg
     9241 aggagaggat gcgccgcttg gccggcggta ccgcagccat ctcggttttc gcccaggcat
     9301 cgagatcctg caattgcttg atgtaggcgg cggcccgcgc gcgatagtcc ggggcgttgg
     9361 ccggatcgct tctggtcagg gccgcggcga tgttcctgac gtagatgacc ccgttcttcg
     9421 ggtcgagcca cgcatgctgg tcgatctctt cggccgcttt gcttcgcggg gcgcgctcct
     9481 cgctggagct gtaggttttc gcgcctctgc tgacgacgat tttggtgccg ccgaacttga
     9541 cgcctttgag cagaggctcg agccatggtt cgaactcgtc gttgagatcg ttcatgaaga
     9601 cgattttggc ttcggcgacg ctcttgccgt cggccaagga tggcttgtag agctcggtgt
     9661 cgccgccggg tccgaccaag gtaacaagat cgacccgatc gccggcgaca ttcgcgacca
     9721 tgtcgccgat gacgctgaac gtcgccacga ccttgatctt gtcggcggcc gcggccgggc
     9781 caatgccgag caggatcccg acacaaacaa agacaaaccg aaacggtgtg ctgattgaag
     9841 cgtcgcgttt catcgctgtt cccccgaacc cctgggtttg atccgcgtcc ggccggccgc
     9901 cagcagcgaa atgccgtaga tcagccccgc gcccattaca atagccggcc ccgagggcag
     9961 gttggcgtaa taagagatga gaatccccgc aagggcggcg gccattccga tcaccgtcgc
    10021 aagggcgatc atcggccata ctttctgggt ccagcaacgc gccgccgctg caggcagcag
    10081 catcggccct atcgcaagca aagtgccaaa agcctggaaa cttgccacca gattgagaac
    10141 caccagcgtg atgaaaatgg cgcggaatag gccaccgccg cctcccgccg cgcgcaggaa
    10201 cgccgggtcg aaggattcga cggccagagg gcgataaagc gctgcgatga tcaacagagt
    10261 tatgctggtt atgcaggcta tcagcagcag cgcattgacg tcgacagcca acaccgtgcc
    10321 gaaaagaaca tgcatcagat ctgcctggct tccccgcgcg gcgacgatca gaatcccgaa
    10381 cgacagcgac accagataga aggcaatcaa gctgacgtcc tggcgctgag acttcgaccg
    10441 tgccatgagc gcggccaggc cagcgacgac aaggccggtc agcagaccac cgaaactcag
    10501 ggcggccagg gaatagccgg cgtagagaaa gccgagtgcc gcgcccggca tcaccgcatg
    10561 cgacaggacg ttgccgctca ggctctcgcg acgcatgagc agcagcgtgc cgatgggccc
    10621 gtttgccaga gccagggcga agctcgcgac cagcgcgatc cgcatgaagc taaatgcctc
    10681 gaacggtccg atcagcaggc caagcagctg tccactcacg cggcacgtcc aaagcgaccg
    10741 ccgtgggaaa gacctggctt cacgtgggcg cgcgctagat tatcagctga aagcgagagc
    10801 gaggtgtccc cccatgcgat gagcgagcgc gccaacagca gcgtcgaggg gaaatgcaga
    10861 cgcacccggt ccaggtcgtg cagcaccgcg acgatcgttt gccctttctc ccgccagcgt
    10921 tgcagcaaat tcagcagctc gtcggttgtg ctctcatcca cggcggcgaa cggctcatcc
    10981 agcaaaatga cgtcggcgtc ctgcaataat agccgcgcga acagggcgcg ctggaattga
    11041 ccgtcggaga gttcggctat ttgccggttg atgaaggctt caaggccaac ggtcgcgacc
    11101 gcctcatgga cccttgtggc aaggcgtggg agcggttcgc ggaccgatcc gaaatttcgc
    11161 cagtcgccga gtgctacgag ttcgccgacc gtgatcggaa agccgcggtc gagttcggcc
    11221 tgctgcggca gataggccag gcgatggcgc gcaagcgcgg cgcaggtgac ctcaccggcc
    11281 attggcctga taatcccggc gagcactttc aagagcgtgc ttttcccggc tccgtttggg
    11341 ccgacgacgg cggtcatgct cgccggcgca aaatctccgg tcacgccctc gagcgcaagg
    11401 tgttcgccat agcccacgga aagattgcgg accgcgattg cgccgtcgcc gcgctgaccg
    11461 ggcccgtcga gcgggctggg atcgtagccg ccatcgaaat ggcgggtgaa ggcggcttgc
    11521 gcgtgatcgc tcagcgccga aagcgaaacg gccagcccac gccgcagcgg tgcgatagcg
    11581 gcgcccccca agttgaccat ctcttggaaa gaccgacgcc agcgcgcgcc atccgaagaa
    11641 ttggccatta caagtccggg tgaaggaatg tcgtaaccgc gcgcgtagtc taggggaggc
    11701 attcaggagg gtcaatcctg gggcatgtga cgccctgggc gcactgccga ctgtgtaccg
    11761 aaaacccgaa gtattgatga gcaacgtctt cacacagccg cgccttgcgc accccaagac
    11821 gtcgacggca tggacaagct cctttaggat gcccttcgtc tttcacaggt gaaaccacgc
    11881 gatgatgtat ctcgacgttc cgccgcgcct catcgagaca aaagtgtttt cggcgatgcc
    11941 cgagcacttc aggcgcaagg gggtgcggac ggactgggcc gatgccaacc ggccgggtca
    12001 gccgaccgac tgctttattg agggcccgtc cttcgacgcc gatggcaatc tctacatcgt
    12061 tgatatcccg ttcgggcgga ttttccggat cgcgcctgac ggcatatggt cgctcgtggt
    12121 cgaatacgat ggctggccca acggtctgaa gattcattcc gacggacgca ttctggttgc
    12181 cgactacagg cacggcatca tggagctcga tgcgcaagca gggcgcatga cacctgtact
    12241 gacggcgcgc aattccgaat cgtttcgcgg ctgcaacgat ctgcacgtcg catcgaatgg
    12301 tgacatctat ttcaccgacg gggacagacc gggcttcacg atcccacggg gcgggtctat
    12361 cggctcgcgc ggagcggacg gctcgactgc ctgatcgata ccggcgtcag ccccaacggg
    12421 ctggtgctcg atcccaccga gagcgtgctc tttgtcgcga tgacccgcga caacgcggtc
    12481 tggcgcgcgc cgttcatgaa ggacggcaat gtttcaaaag tcggccgctt ctgttcgacc
    12541 ttcggcccga gcggtcccga tgggctgacc atggacaagg ccggccggct gttcgtggcg
    12601 cacgcctcgc tcgggcatgt gttcgtgttt gcgcccaatg gcgagttgat cgcgcgcatc
    12661 aaatcctgcg ccggaccaag ctgcaccaat gtcgcgatcg gcggcaagaa ccgcgaccgc
    12721 ctctatgtga cggaatcagt gacgggcacg gtgctggtcg cggatatcag cgttctcaat
    12781 tcgttatcgc agggagtgtg agcgacgatt aggccggctg ttcgacaaac tccagagcca
    12841 ggccatggca cgctgccggc gggatcaaga gaacgccgcc gttgatctgg aagtcgaccg
    12901 cattgcgctg caactctcgc ctggtcgcat ccagatcggc gacggccaac cgcgccgcgc
    12961 aaaaacgcgg cggaccgtcg gcagccaaac cgggcagctg acgggcgatc tcaagggggc
    13021 tgagcacggt gatccggtcg cctgcctctt cgaccgttag tcgcccgggc gcaagctcgg
    13081 ctgagctttc ggtgatgcgc tcaaggaagt tttggtgcgc cgccggctcc ggcgccgaca
    13141 tcacgacctc gatcacgcgc aaggcgccgt tggggtggcg ctggtagtcc ggcttccaga
    13201 acagttccgg cggatgacgc tgctggcagg tgaaaaaagc gatcccgggc atggcgggat
    13261 gggtggcgaa ggcgagtgaa aatccaaccc gagcggtcgc tccgtccggg agcaccgcgt
    13321 cacggccgaa atcaaacggc gcataggcgc cgatgccctt tgccttgaag cgcgccgcat
    13381 cggcatgcgc gtcggtgctg tgccaggcca gcatcgacat gccctccgcc gcggcgagaa
    13441 aatcccggtt gtgagctgca aagctgaatc ggcagggggc ggcggccggt attgccgcgg
    13501 cgtcggtgac ggccagcagt tcgagaaagc tgttcgcaaa cattgccagg cggttgctgg
    13561 ttccgaacgg atgaacgccg accggcgtga gggtaaagcc gagggctcgc caactcagcg
    13621 cagcctgcgc aagatcgcgg acgcaaatga tgaggtggtc gatgcggcgg ggcatgggag
    13681 tctacgggct tgtgaaccgg acggtagctt gtgctgtgaa cgcagggcaa agcattgttc
    13741 ctgacgcgca gctgaagaaa atctattgca aggccagcac gtatcggtga caaactgggc
    13801 ggcgatgacc tacagcctcc caccgcttaa tgcgctccgc gccttcgaag ccgcggcgcg
    13861 acatctcagt ttcaagctgg cggcgcacga gctgcatgtg acacctgccg ccgtcggaca
    13921 gcaggtgaaa gccttggagg cgcggctcgg cgtgcgtctg ttcgagcggc tgcataagca
    13981 gcttattctg acgtcggccg gccaaatctt cctgcccgaa atatccgccg gctttcgtcg
    14041 tatcgccgat gcgaccgcgc aattgaggcc gtcgggcgcg gtgctgctgc agttgggtct
    14101 gcacggaagc tttgacctcc gccgcctggc gcttgccgag ttccgtgcga cgcatgccga
    14161 aatcggcttg cgcgattggc tgcgccggct gccggccgag aacgcgctgg taatggatcg
    14221 ccgccgtgcg tcggcaagcg tcagccaaag ttgatcgagc acgatgccgg caagtcctgc
    14281 gtcggatcga gctgtacaaa tgtcgcaatc ggcggcaaga atcgtgacca tgaaatctcg
    14341 gccgttcggc aacagcaaat gtgaggtgtc cgtgatcggg cagggcacct ggtacatcga
    14401 ccgcggagat cgccgaagcg cggtcgccgc gttgcgccgc ggcgtcgatc tcggcatgac
    14461 ccatattgac accgccgaga tgtatggcga cgccgagccg gtgatcgccg aagcgatcgc
    14521 cgagcggcgc gaggagattt tcctggtttc gaaagttctg ccgagcaacg catcgcggcg
    14581 cggcaccatc acggcgtgcg agcgttcgct ggcgcggctg aaaactgacc ggctcgattg
    14641 ctatctgttg cattggcgcg gatcctattc gctggatgag actgtggccg cgttcgatca
    14701 tttggtcgat gcagggaaaa tccgcagctg gggcgtcagc aatttcgata ccgacgatct
    14761 cgatgaactg ctggcggttg ccggcgacgg caagatagcc tgtaaccagg tactctatca
    14821 tctcggggag cgcgcgatcg agcacgcggt gattccgtgg tgcgagcagc acggcgtcgc
    14881 ggtggtcgcc tattcgccgt tcggtcacaa cgattttccg ccggcgcgca gcaaggcggg
    14941 gcaggcgctg caggctatcg ccaaagccca tggcgcatcg ccgcgccagg tcgccttgag
    15001 ctttctgacg cgcgctactt cggtattcgc gattccaaaa gcgtcaagtc aggaacacgc
    15061 cgccgacaac gcggcagcgg caaatctcac gctcagcgaa gacgagatca ccgcactgga
    15121 caaggcattt ccgcgtggtc caaagccgcg tggtctgccg atgctgtagc agacacggct
    15181 cctcacaaaa tgtttcagcg gtgacgcggc cagccgccgc acgcgatcac gcatatcggc
    15241 tgcctgtttt cggcccttgt cgatgactgg caattatcgt tttttgagct ctcgcccgat
    15301 tcgatcatta cgcccgagtt tcacttgtga ccccgcctcc cgccgcagcc gccaggctgg
    15361 acagcaacgc attgtcgttg cccgagaaga agttatccga acgaaccgca cccgcgcgcg
    15421 ccgaccggcc gtttcgcggc atcgcgctga tccttgcgtc gaccgtgttt ctcggggcct
    15481 cggacgccac cgcgaaatat ctctcggcga cgttgccgtc gatcgagatc gcctggatca
    15541 ggtttctggt tttcgggatg atcatgtttc ccgcgatgct gccggggtcg ccattgtatg
    15601 ctttgccatc cgggcgccgc ggcctgcagt tgatgcgcgg cgccgcgctg ctggcatctt
    15661 cgctgttttt catcaccggc ctcggatttc tcccgatcgc ggaagcatcc gctaccggat
    15721 ttgtctcgcc gctgttcgtc accgcgctgt cgattgtgtt tctcggagag agcgtcggcg
    15781 tgcgccgctg gctcgcgacc gcggtcggat tgatcggcgt gctgattatc ctgcggccgg
    15841 gctcaagcgc gttccatccc gccgcgttct ttccgatcgt ctcggcgctg gcctgggcct
    15901 gcactatgat catgacgcgc atgatgagcg gcaaggaacc cgtcatcacc accatgacct
    15961 attcgtcgat cgcgggcgtc tgcatcttgt cagcactggt cccgtttgtt tgggtcgcgc
    16021 caagctggca tgatgtcctg ttcggcatct tcatcggcat cgcctcgacg gcgggccaat
    16081 ggatcgtggt gctcgcgttt cgttatgccg atgcctcggt gctcgcgcca tttcagtaca
    16141 cccagctgct gtgggtcagc atcctcggct tcatgatctt cggcgaacta cctgatatct
    16201 ggaccgtgac cggcgcggtg ttcatcgtcg ccagcggtct ctacaccgcg caccgggagc
    16261 gtatcaggcg ctcgcagctt ccaatgcagg ccgagccgtc gcccaacgcg tgacccgccg
    16321 acgttctgtc acgtcgacgt ccgcgctggt cggcgggggc gttctgccgg aactgacgta
    16381 ctgcgcggcg gtcgagcagt tcggccagca cggggcggcg gaactgtcct gtctcgtcgg
    16441 actgtattgc ctgttgtcgg tgaccttgaa tacgttcgag gtcccggtgc cggagtccag
    16501 agcgcctacg acgcaggcgg ttagcagtct tgcccggcac acgttacatg ttttgaacct
    16561 ccgtggtatg agggcgggca atcacggacc gcgagggggt cgccgatgcg cgctgccatt
    16621 ttcaggaacg gcgagatcgt cgtcgatacg ctgcccgagc ccaaacccgc cgcgggacag
    16681 gtgctggtca agacgctggc ctgcggcatt tgcggctcgg acctgcatgc ccgcaagcac
    16741 gcccaccaca tggtcgagct ggcacggcat ctccccggcc gcaagtcgat ggacctcacc
    16801 cgcgatgtcg tgttcggcca tgaattctgc tgcgagattc tcgactacgg cccggccacg
    16861 tcgcgcaagc tgaaagccgg cacgcgggtc tgctcgctcc cggcgttgct gacatcggat
    16921 ggcccgcagg gcatcggcta ttccaacgac aatattggcg gctatgccga gcgcatgctg
    16981 ctgagcgagg cgcttttact ggaggtgccg aacgggcttg cggcggaaca cgcggcgttg
    17041 accgaaccgc tcgccgtcgg tgttcacgca gtggcaaaag ccaatgtgcg tggcgacgag
    17101 gtgccgctgg tgatcggctg cggcccggtg ggcttggctg tgatcgcggc gctgaagctg
    17161 aaaggcttgc atccgatcgt ggcggcggat tattcgccgg cgcgccgggc cttggccgag
    17221 aaactgggcg ccgacatcgt cgtcgatccc gcgcgcaacg aaccttacgc gacctgggcc
    17281 gagcacgccg cgatgtcgcc cgagcaaaag gccgcacggc cgccgttgca cgcgtggcgg
    17341 ccggccttga aaccggcgct gatcttcgaa tgcgtcggga taccaggcct gctgcagcag
    17401 gtctttgaag gcgcgccgcg cgacgcccgc attgtcgtgg tcggcgtctg catggaaacc
    17461 gatcgctccg agccgaccct cggcatcatg aaggagctca acgttcaata tgtgctgggc
    17521 tatacgcccg acgagtttgc ctcttcgctg cgcctgatcg cggaagggca ggtcgatgcg
    17581 gcctcgctcg taaccgcgac cgtcggcatc gacggcgtcg ccaccgcgtt cgctgacttg
    17641 gccaatccgg aacgtcacac caagatcatc gtcgagccgt ggcggtaagc gattccgatc
    17701 gtccgcccgt tctcgcgcat cagcgctttg ttggggacag ggaatccttt gcgggcctga
    17761 gcctggagca gcgcttctgc cgcatccacg acaccaacct gtggggtgct gtggcatcca
    17821 cttccggatt gggctcggag atcgacgcca ccgccatgct gcgcgcggaa ttaccgcggt
    17881 tgctcgaaaa actcggcgtg accacactgc tcgatgcgcc ctgcggcgac gccggctgga
    17941 tcaatcaggc gaatttgggc gtgcgcgcca tcggcgtcga tatcgtgcct gcgctgatca
    18001 agcgcctgca ggcccgtgcg gctgccgggg aaatcaaggg cgaatatcat ctcgccgata
    18061 tcacccgcga cccgctgccg cgatgcgacg cggtcctgtg ccgcgacgcg ctcgtgcatc
    18121 tgtcgtttga aaatatcgaa cgcgcggttg cgaatttcaa ggcgtccggc gcggtgtggc
    18181 tgatccgcct ggtgcttgct tgcgttgtcg gcctcgatgc tgcgcgtggt cgtcgtcaag
    18241 gttgccgcca gcgcgttgct ggcagcgatt tttgccgctt gcgtttcgat ctggccgctg
    18301 tcgctgatgc gcaaagcggt ggacgatctg gaggccgcac ggctggagaa atacgacgcg
    18361 gatctcgccc gggtgccggc gaatgctccg ttgtgggaat ggacgccatt cctcgagacc
    18421 caagacggta ccaagcaggc gaaggcgctc gacagcattc gcagtatcga ccagcgccag
    18481 gctcaagccg aagccatgct cgaccgcggc gacttcccca ttggctatct cgggtctttc
    18541 gatctcgatc caacgcctgc gctttgcgag aaggcgcgca gcctgcttcg caggcgcgtc
    18601 gaaccgcttg tgctcaaaac ccccgatccc aaaccgtatt ccgatatcgc gctgcccttg
    18661 gcagatgcca tctcaggcat gaactggctg gtcggctatg actgcgcgtg cgacgccgaa
    18721 gcggcggcat gggaagccat ggccaaggga tatagtaaca ccagcctgga cgtccatcat
    18781 ctggctgagc ttcgcgatcc caaggagttg gggcgaacgc tgcgcgagcg ccccgagcgc
    18841 ttttcaatgt tgaacgcgaa gtcgcacctg aaggggtggc tgagatttgc cgacgacgat
    18901 agcttgcatg atcgggccct gaccggcgct cgcgcgctcg accaccgcac caccgacgct
    18961 gtcgagatat tgatcgataa atatgacgag gaaagccgat ggaagctgtt gcggtatctt
    19021 accgcgctcg acctcgatgc gacgatgccc ttgtgcgcga acctgctcag cgagttgcat
    19081 ggtcagttca cgaaggttta ccggccgggc gcgaatgatg agccacggcc ttattccgaa
    19141 ttgctcgagc ggttgggaaa cggcgagcaa ctgccagacc tgatatggct ggccggacac
    19201 ggctgcgcgg ctgagccgga attgagcgag gcggtggcgc tggtgaatgc ctatcgggat
    19261 tcgcccggca gagcagagat gctgaccacg ctgacgcggc tgcgcgggcg gcggtgacga
    19321 aagatcacac cggcagcgcg atcgagtatt tcacctggct caacgcaaag ctcgactcga
    19381 tcgaggcgat gccgtcgagc cgggtcagct tgttcttcaa gaacgcttca tacgacgaga
    19441 gatcggcagc gacgacgcgc aacagataat cgcggttgcc ggtcatcagg tagcactcca
    19501 aaacctcgtc ccatttcgag atcgctttcg cgaaccggtt gaggtcttcc tccttctgcc
    19561 gcgccagctt gatcgagatg aacacgctga catgcaggcc gagcgatttc tggtcgacgg
    19621 ttgcgatgta gcgggtgatc acgccgcgcg cttccagaag tttcacccgg cgatggcagg
    19681 gcgagaccga caggccgacc ttgtcggcga gttcctgcat ggtggtgcgg ctgtcggatt
    19741 gcaactggct gaggattttg cggtcgatgg cgtcaagcgt atgcattggg ataaactcgg
    19801 gctggcgggc ttcgaagtgg gattttatcc caatttaggt cgggatgtcg cgagaatttg
    19861 agatttctgg cgaagaccga cgcgctacca ttggcggata atcattgtgc cggggaatac
    19921 cgccatgtcg atcgagcccg cacgcctcga attgttgtct gcgctcgccc gcaaagtctt
    19981 gtggctgtcg tcgtggacca tccatcacgc caatcacgtc aggcccaatg tcgacggcct
    20041 gaaagtcggc ggccaccagg cctcgtcggc ctcgctcgcc accatcatgt cggcgttgta
    20101 tttcgcagct ctgcggccgg aggaccgcgt cgcggtgaag ccgcatgcga gcccggtatt
    20161 ccacgccatc cagtatttgt tcgggcacca gacccgcgac aagctggaaa actttcgcgg
    20221 tttcaagggc gcgcagtcct atccttcgcg caccaaggac accgacgacg tcgatttctc
    20281 cacgggatcg gtggggctgg gcgttgcgca gacgctgttc tcctcgctgg tgcaggacta
    20341 cgtcaaggcg catggctgga tgaagcggcg gcccgaggga cggatgatcg cgctggtcgg
    20401 cgacgccgag atggacgagg gcaacatctt cgaggcgctg ctggagggct ggaagcacgg
    20461 gttgcgcaac acctggtggg tggtcgacta caaccgccag agtctcgatg ccgtggtgcg
    20521 cgagggactg tgggaaaaat tcgaatccat gttccgcaac tttggttggg acgtggtcat
    20581 cgtgaagtac ggcaagctga tgcaggcggc tttcgccgaa cccggcggtg aggccctaaa
    20641 acgctggatc gacgattgcc cgaacgcgat gtatgccgcg ctgtgctttc agggcggggc
    20701 ggccttccgc aagcatctca cggacgagat cggcgatcag ggcgcggtgt cgcggctgat
    20761 cgacaagcgc agcgacgatg aattgttggc gctgatgtcc aatctcggcg gtcacgacat
    20821 ggccagcatg gtcgaggcct tcgaggccat cgatcacgac cggccggtct gctttatcgc
    20881 ctacaccatc aagggcgtcg gcctgccgtt ccagggccac aaggacaatc atgccggctt
    20941 gatgaccgtc gcccagatgg aaaaatggcg cggcgcgcag aacatccggc cgggtcacga
    21001 atgggaaaaa ttcgagggcc tgtctcagga tccggcggcg ctggaggcgt ttcttggcca
    21061 ggcgccgttc aatcgcgacg gccggcgccg cctgaccgct gatgtgatcg agg
//
LOCUS       DATNOI010000626         7053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1080294,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000626 DATNOI010000000
VERSION     DATNOI010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080294"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..94
                     /locus_tag="VN968_25375"
     CDS             <1..94
                     /locus_tag="VN968_25375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXL32594.1"
                     /translation="NFVSALVWSAALLLFGDVIAQVLEWLWQVV"
     gene            272..1261
                     /locus_tag="VN968_25380"
     CDS             272..1261
                     /locus_tag="VN968_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL32595.1"
                     /translation="MELTRRHALAGAAAIAATPLLPSVPVRAAAPVADKQAPSFYRYK
                     VGDIQVTVVSDGKNVFKLEDSFITNAKKEEVNAALEKAFLPRDIMSIYFAPLVINTGG
                     KLVVLDTGNGAVAKTNSKGANGLFADNMAAAGIDANAVDMVVISHFHGDHVNGLLTAD
                     GTPAFPNAEVLVPATEWKFWMDDGEMSRATGARMTGLFKNNRNIFEAALKKKVTPYEW
                     GKEVAPGMLAVETAGHTPGHTSYVLSSGSGKVFIQSDVTNNPNPFATNPGWHAFFDQD
                     GDMAETTRRRVYDMVVAEKLQVQGFHYPFPGLGNVEKDGSGYRVIPAPWNPTI"
     gene            1585..2250
                     /locus_tag="VN968_25385"
     CDS             1585..2250
                     /locus_tag="VN968_25385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HXL32596.1"
                     /translation="MFTRPFFEPDRAASLAFADARGFGTVCACDRGKPVASSLPFYLS
                     YGDDGTPHALFHVARQNALASLADGKSSWLIAVNGADAYVSPDWYASPDQVPTWLYQA
                     VHLTGTVRRLSDHELGPHLDALSAKFESWLMPKPPWTSSKMTAGRLDAMKKAIVGLVM
                     TVEEIEGSFKLNQHKSDVDHAAIADALALQADAGAQQLARTMRTMRPQAFTDETVQLE
                     RSV"
     gene            2250..3299
                     /gene="argC"
                     /locus_tag="VN968_25390"
     CDS             2250..3299
                     /gene="argC"
                     /locus_tag="VN968_25390"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HXL32597.1"
                     /translation="MSTKKKIGILGASGYTGADAVRLLARHPDAEIVALTANTHAGKA
                     MDEVFPHFFMLELPKLVEWEKVDWARLDAVFCGLPHGTTQEIIAAVLAANPAIKILDM
                     SADFRLRDMSAYAQWYGHEHRAPELQDEAVYGLTELYREKITAARLVACPGCYPTAAL
                     LALVPIAKAKLIDVDDIVIDAKSGVTGAGRGLKQNTLFSEAGEGLSPYSIASHRHSPE
                     IDQEIGVAAGTKMTVNFTPHLIPMSRGELCTSYVKLNGASADDLRAALAEAYKDEPFV
                     HVAKKGVLPQTQNVRGSNYVQIGVFADRIKNRAIVISVLDNLVKGSAGQAIQNMNLML
                     GFPETTGLEQIALFP"
     gene            complement(3304..3606)
                     /locus_tag="VN968_25395"
     CDS             complement(3304..3606)
                     /locus_tag="VN968_25395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL32598.1"
                     /translation="MTQDAIKYGAAAHLRSVIVGNAIELTVDDEGLGIPPERRQAMLE
                     PFSRLETSRNRGTGGAGLGLAIARGLVEAHGGSLSIGQAPSGGARITVRLPLFQSN"
     gene            complement(3603..4001)
                     /locus_tag="VN968_25400"
     CDS             complement(3603..4001)
                     /locus_tag="VN968_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HXL32599.1"
                     /translation="MTRELFWLTLTVIFTGLLWIPYLVNRCQVRGLSGTMANPSRNDK
                     PHAEWATRLMFAHDNAIENLIIFAPLVLILAEIDYSTTWTVYACAVYFWARVAHLIVY
                     TLGLPVFRSLAFTVGFLAQAVLALAIFKIV"
     gene            complement(4076..5881)
                     /gene="phaC"
                     /locus_tag="VN968_25405"
     CDS             complement(4076..5881)
                     /gene="phaC"
                     /locus_tag="VN968_25405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HXL32600.1"
                     /translation="MSDVSTETQTEKTFDPEAFAMNLARAMESSGQALAAYLKPRENG
                     DAKDKPPSELTEVIKTFSTIAEYWLSDNARVSELQMKMGKAYLDLWGSSMRRLVGEQA
                     RPAIEPSPRDKRFKDPEWKSNQFFDFVMQLYLLTTQWAHDLVHNAEGVDPHTRKKAEF
                     YVAQITNALAPSNFVLTNPEILRETLASSGGNLVRGMKMLAEDIEAGQGTLRIRQSDP
                     SNLIVGVNMATTPGKVIFQNELMQLIQYAPSTENVLRTPLLIVPPWINKFYILDLKPE
                     KSFIKWCVDQGITVFVISWVNPDKSLGSKTFDDYMREGPLAAMDAIEQATGELKVHTM
                     GYCVGGTLLASTLAWLAEKRRVRVTSATFLAAQVDFSHAGDLLVFVDEGQIAALERDM
                     QASGVLEGSRMAMAFNMLRSNDLIWSYVVSNYLKGQPPSAFDLLHWNSDATRMPAANH
                     SYYLRNCYLENRLSTGSMVLDNTLLDLTKVKVPIYNLATREDHIAPADSVLYGSQFFG
                     GPVKYVLSGSGHIAGVVNPPSAGKYQFWTNDHIKDIALADWLKGAQEHKGSWWPDWRE
                     WLQGIDAEEMPARTVGTDALPAIEDAPGSYVRVRA"
     gene            6120..6428
                     /locus_tag="VN968_25410"
     CDS             6120..6428
                     /locus_tag="VN968_25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32601.1"
                     /translation="MGTSRPVPVDRKISALAVGLLLLSALPLGGCATSIAEMPIVGTP
                     ADAPARPKEAGTYLPVHDLPPDREEAEMTPAEQAKALKELAAARDHQASVAAKNPAAK
                     "
     gene            6611..>7053
                     /locus_tag="VN968_25415"
     CDS             6611..>7053
                     /locus_tag="VN968_25415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXL32602.1"
                     /translation="MEDFYRIRRLPPYVFEQVNRAKAAARNAGADIIDLGMGNPDLPT
                     PPHVIEKLKETLGKPRTDRYSASRGITGLRKAQAAYYDRRFGVKLNPETQVVATLGSK
                     EGFANVAQAITAPGDVVLCPNPSYPIHAFGFLMAGGVIRSVPSEPT"
BASE COUNT         1369 a         2178 c         2173 g         1333 t
ORIGIN      
        1 gaatttcgta tcggcgctgg tctggtcggc ggcactgctg ctgttcggcg acgtgatcgc
       61 gcaagttctc gaatggctgt ggcaggtggt ctgacgcggc aggcccggaa taagccgcgc
      121 gcaggggtgt tagcccctcc aatagcatag gttgcaatgg cgtcgacgtg atcgccctgc
      181 ggccaaaacg ctggtaaaac tgaccacgat cagaagcaag aaagcgggaa atcccgcacc
      241 aaagtcatcg tctcgctggg aggacaccat catggaacta acacgacgtc acgcgcttgc
      301 cggcgccgca gccattgcgg ccacgcctct cttgccatcc gtgcctgtga gagccgccgc
      361 cccggtggcg gacaagcagg cgccgagttt ctatcgctac aaggtcggcg acatccaggt
      421 cacggtggtg tccgacggca agaatgtctt caagctcgag gacagcttca tcaccaatgc
      481 caagaaggaa gaagtcaacg ccgcgctcga aaaggcgttt ctgccgcgtg acatcatgtc
      541 gatctatttc gcgccgctgg tgatcaacac cggcggcaag ctcgtcgtgc tcgataccgg
      601 caatggcgcg gtagccaaaa ccaacagcaa gggcgccaac ggattgttcg ccgacaacat
      661 ggccgccgcg ggcatcgatg ccaacgcggt cgatatggtg gtgatctcgc acttccatgg
      721 cgatcacgtg aacggcctgc tgacggccga cggcacgccg gcgtttccca acgccgaagt
      781 gctggtgccc gcgaccgagt ggaaattctg gatggacgac ggcgagatga gtcgggccac
      841 cggcgcgcgg atgacggggc tgttcaagaa caaccgcaac atcttcgagg cggctctgaa
      901 aaagaaggtg acgccgtatg agtggggcaa ggaggtcgcg ccgggaatgc tcgcggtcga
      961 gaccgcgggg cacacgccag gccacacttc ctatgtgctg tcatcgggtt cggggaaggt
     1021 gttcatccag tcggacgtga ccaataatcc gaatccgttt gcgaccaatc cgggctggca
     1081 tgcgttcttc gaccaggacg gggatatggc cgaaacaacc cgccgccggg tctacgacat
     1141 ggtggttgcc gaaaaactgc aggtgcaggg cttccactat ccgttcccag gcctcggcaa
     1201 tgtcgaaaag gacggcagcg gttaccgggt gattccggcg ccgtggaatc cgacgatctg
     1261 attcgtcatt ccggggcgac gcgcagcgtc gaacccggaa tctcgagatt ccgggttcgc
     1321 gctccgcgcg ccccggaacg acgtcggttg agttcacttg acccggcctc aatcatcttg
     1381 acgaaaaccg ggcgcggcct tatagccgcg cttatgatca ataccgcgac catcctgatt
     1441 gctcgccgcc gccgtaccat tgcggtatgg gcggacatct gcgcttaaga tttcagcctc
     1501 tgccgccgga tccggtccgc ggcacttcct tccaaaccag cgcgatctga tcccggcggc
     1561 tccctcaacc aagcaggagc agccatgttc acccgacctt tctttgagcc cgatcgcgcc
     1621 gcaagcctcg ccttcgcgga cgcgcgcggt ttcggcacgg tctgcgcctg tgatcgcggc
     1681 aagccggtcg catcctcgct gccgttctat ctcagctatg gcgacgacgg cacgccgcac
     1741 gctctgtttc acgtcgcccg tcaaaatgcg ctggcgagcc tggcagacgg gaaatcatcc
     1801 tggctgatag ccgtcaacgg cgcggatgcc tatgtttcgc ccgactggta cgcctcgccg
     1861 gatcaggtgc cgacctggct ctatcaggcc gtgcatctca ccggcaccgt gcggaggctt
     1921 tccgatcacg aactcggtcc acacctcgac gcgctgagcg cgaaatttga aagctggctc
     1981 atgccaaaac cgccctggac gtcgtcgaag atgacggccg gccggctcga tgcgatgaag
     2041 aaggcgatcg tgggtctggt gatgacggtt gaagagatcg agggcagttt caagctcaac
     2101 cagcacaaat ccgacgtcga tcatgcggcc attgccgatg cgctggcttt acaagccgac
     2161 gccggcgcgc aacagctcgc gcgcaccatg cgcacgatgc gcccgcaagc ctttaccgac
     2221 gaaacagtac agctcgaaag gagcgtgtga tgagcacgaa gaagaagatc ggcattctcg
     2281 gcgcatccgg ctacaccggc gccgatgcgg tgcgcctgct ggcgcggcat cccgatgccg
     2341 aaatcgtcgc gctcaccgcc aatacccatg ccggcaaggc catggacgag gtgttcccgc
     2401 atttcttcat gctggagctg ccgaaactcg tggaatggga aaaggtcgac tgggccaggc
     2461 tcgatgcggt gttttgcggg ctgccgcacg gcaccacgca ggagatcatc gcggccgtcc
     2521 tcgccgccaa tccggcgatc aagatcctcg acatgtcggc ggatttccgg ctccgcgaca
     2581 tgagcgcata cgcgcaatgg tacggccatg agcaccgcgc gccagaactt caagatgagg
     2641 ccgtctatgg gctgaccgaa ctctaccgcg agaaaatcac cgcggcgcgg ctcgtcgcct
     2701 gtcccggctg ctatccgacg gcggcactgc tggcgctggt cccgatcgca aaggcaaagc
     2761 tgatcgatgt cgacgacatc gtcatcgatg cgaaatcagg cgtcaccggt gctggccggg
     2821 gtctgaagca gaacacgctg ttcagcgaag ccggcgaggg gctttcgccc tattccattg
     2881 cctcgcatcg gcactcgccg gagatcgacc aggagatcgg cgtcgccgca ggcacaaaaa
     2941 tgacagtcaa cttcacgccg catctcattc caatgagccg gggcgagctc tgcacgtcct
     3001 acgtcaaact caacggcgcg agcgcggacg atctgcgcgc agccctcgcg gaggcctaca
     3061 aggacgagcc gttcgtgcac gtcgccaaga agggcgtact gccgcagacg cagaacgtgc
     3121 gcggctcgaa ttacgtgcag atcggcgttt tcgccgaccg catcaagaac cgcgcgatcg
     3181 tcatttcggt gctcgataat ctggtgaagg gctcggcggg gcaggcgatc cagaacatga
     3241 acctgatgct cgggtttccc gaaaccacgg gactggagca gatcgcgctg tttccgtaag
     3301 ccgtcagttc gattgaaaca gcggaagtcg gaccgtgatc cgggcgccgc ccgatggggc
     3361 ttggccgatc gacaggctgc cgccgtgcgc ctcgaccagg ccgcgcgcga tcgcgagacc
     3421 cagtccggcg ccgccggtgc cgcggttgcg cgaggtctcc agccgactga acggctcgag
     3481 catcgcctgc cgccgctccg gcggaatccc caagccttcg tcgtcgaccg tgagttcgat
     3541 ggcgttgccg acgatcacac tgcgaagatg cgccgctgcg ccgtatttga tggcatcttg
     3601 cgtcaaacga tcttgaaaat cgccagcgcc agtacggcct gcgccaaaaa gccgacggtg
     3661 aaggccagcg accggaacac cggcaggccc agcgtgtaga cgatcagatg ggcgacgcga
     3721 gcccagaaat agaccgcgca ggcatagacg gtccacgtgg tcgaatagtc gatctcggcc
     3781 aggatcagca ccaagggcgc gaagatgatg aggttctcga tcgcattgtc gtgcgcgaac
     3841 atcaggcggg tggcccattc cgcatgcggc ttgtcgttgc gcgacggatt ggccatggtg
     3901 ccgctgaggc cgcgaacctg acagcgatta actaggtagg gaatccacaa tagtcccgtg
     3961 aaaatgaccg tcagcgtcag ccagaacaat tcgcgcgtca tggggttccc ctcaatcgag
     4021 tttcaagtct cgtcccttcg ggacggcctg ttatattcaa atgcgcatcc cagcgctacg
     4081 cgcggacgcg gacgtagctg ccgggtgcgt cttcgatggc gggcagtgca tcggtaccga
     4141 ctgtgcgcgc gggcatttcc tcggcgtcga tgccttgcag ccattcacgc cagtcaggcc
     4201 accacgatcc cttatgctcc tgtgcgccct tgagccagtc cgccagcgca atgtccttga
     4261 tatggtcgtt ggtccagaac tggtacttgc ccgccgatgg cgggttgacc acgccggcga
     4321 tatgcccgga gccggacagc acgtatttca ccgggccgcc gaaaaactgc gagccgtaga
     4381 gcaccgaatc cgccggcgcg atgtggtcct cgcgcgtcgc cagattgtag atcggaacct
     4441 tcaccttggt cagatcgagc agcgtgttgt ccagcaccat gctgccggtc gacagccggt
     4501 tctccagata gcagttgcgc aggtaatagg agtggttcgc cgccggcatc cgggtcgcat
     4561 ccgaattcca gtgcagcaga tcgaacgccg agggcggctg tcctttcagg taattgctga
     4621 cgacatagga ccagatcaaa tcgttcgagc gcagcatgtt gaaggccatc gccatcctgc
     4681 tgccttcgag cacgccgctg gcctgcatgt cgcgctccag tgcggcgatc tggccttcgt
     4741 cgacgaacac caggagatca ccggcgtggc tgaaatccac ctgcgccgcc agaaatgtcg
     4801 ccgacgtcac ccttacccgc cgcttctcgg ccagccacgc cagcgtcgag gcgagcaggg
     4861 tgccgccgac gcagtagccc atggtgtgca ctttcagttc gccggtggcc tgctcgatgg
     4921 cgtccatcgc cgccaatggg ccttctctca tatagtcgtc gaaggttttg cttcccaggc
     4981 tcttgtcggg gttgacccag gaaatcacga acaccgtgat gccctggtcg acgcaccatt
     5041 tgatgaagga tttttcaggc ttgaggtcga ggatatagaa tttgttgatc cacggcggca
     5101 cgatcaaaag cggcgtgcgc agcacgttct cggtcgaagg cgcgtactgg atcaactgca
     5161 tcagctcatt ctggaagatc accttgcccg gcgtggtcgc catgttgacg ccgacgatga
     5221 gattggaagg atccgactga cggatccgca gcgtgccctg tccggcctcg atgtcttcgg
     5281 ccagcatctt catgccgcga acgaggttgc cgccgctgga ggccagcgtt tcgcgcaata
     5341 tttccggatt ggtgaggacg aaattcgatg gcgctagcgc gttggtgatc tgcgcaacgt
     5401 agaactcggc cttcttgcgg gtatgcggat cgacgccttc ggcgttgtgg acgagatcat
     5461 gcgcccactg cgtcgtcagc agatagagtt gcatcacgaa atcgaaaaac tggttcgatt
     5521 tccactcggg gtccttgaac cgcttgtcgc gcggcgacgg ttcgatcgcc ggccgcgcct
     5581 gttcgccgac gaggcggcgc attgatgagc cccacagatc gagataggcc ttgcccatct
     5641 tcatctgcag ttccgaaacc cgagcgttgt ccgacagcca atattccgca atggtgctga
     5701 aggtcttgat cacctcggtc aactcgctgg gcggcttgtc cttggcgtcg ccgttctcgc
     5761 gtggcttgag ataggccgca agcgcctggc cgctgctctc catggcccgc gcgaggttca
     5821 tcgcgaaggc ttcgggatcg aacgtcttct cggtctgggt ttcggtggaa acgtcgctca
     5881 tgtccggacc ctacaggttc gctcagggtt cgtcaccgcg ttgatcacca tgaacggttc
     5941 cggattaacc ctgacatcac atcctgttgc actgcgacaa agtatcctgc ttctgtcaca
     6001 ttgaagcccc agcggttcga tttcttgttc aggacttaat cgtttagagc gacaaaggtt
     6061 gagcctgggc cgacaactga tgatataatt atcaggctgc gaagcttggg gttggcgaag
     6121 tggggacttc cagaccagtg ccggtggatc gaaaaataag cgcccttgcg gtgggactgc
     6181 tgttgctgtc ggcgctgccg cttggcggct gcgcgacatc gattgcggaa atgccgattg
     6241 tgggcacccc ggccgatgcc cccgcacgcc cgaaggaggc cggcacctac cttccggttc
     6301 atgatttgcc gccggaccgc gaggaggccg agatgacccc cgcggagcag gccaaggcgt
     6361 tgaaggaact ggcagccgcc cgggaccacc aggcttccgt ggcggccaaa aaccccgccg
     6421 caaagtgagg cttggcgtac tattacggct ggcgcagccg ggcgtgcgtg gtagaacaag
     6481 cccgattcaa cggtttggtg ccgaatttgc ccataaatca ttgattttgc gcgctttctg
     6541 cgcggaatcc gattccgctc tggcgccacc tgacgatctt gccctgaccg gcttccggag
     6601 cttgagaccg atggaagatt tttaccgcat tcgccgtctg ccgccttacg tgttcgagca
     6661 ggtcaaccgg gccaaggcgg ccgcgcgcaa tgccggggcc gatatcatcg atctaggcat
     6721 gggcaatccg gatttgccga cgccgcccca tgtcatcgaa aaactgaagg aaacgctggg
     6781 caagccgcgg accgatcgct actccgcgtc gcgcggaatt accggtttgc gcaaggcgca
     6841 ggcggcctat tacgaccggc gttttggcgt gaaactgaac cccgagaccc aggtcgtggc
     6901 gacactgggc tcaaaggaag gttttgccaa tgtggcgcag gcgatcaccg cgcccggcga
     6961 tgtggtgctg tgcccgaatc cgagctaccc gatacacgcc tttggattcc tgatggccgg
     7021 cggcgtgatc cggtcggtac cctcggagcc gac
//
LOCUS       DATNOI010000627         4566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1500068,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000627 DATNOI010000000
VERSION     DATNOI010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500068"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2602
                     /gene="secA"
                     /locus_tag="VN968_25420"
     CDS             <1..2602
                     /gene="secA"
                     /locus_tag="VN968_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HXL32603.1"
                     /translation="TLGQRHFDVQMIGGMVLHEGDIAEMKTGEGKTLVATLAVYLNAL
                     AGKGVHVVTVNDYLARRDSEWMGQIYNFLGLSVGVIVHGLDDAERKNSYGCDITYGTN
                     NEYGFDYLRDNMKYRLEDMVQRGHFYAIVDEVDSILIDEARTPLIISGPLDDRSEFYN
                     TIDTFFPKLDKTDYDVDEKQRTVTLTEAGMEKIETLLRDAGQLKGDSLYDVENVSVVH
                     HINQALRAHSLFQRDKDYIVRDDEVIIIDEFTGRMMQGRRYSEGLHQALEAKEHVQVQ
                     PENQTLASITFQNYFRMYEKLSGMTGTALTEADELFDIYKLEVVEIPTNVMVARLDED
                     DEVYRTQTEKYAAILAEVERANVRLQPVLVGTASIEKSEVLAEYLKKNGYKQIDFGNE
                     NAMEKLYAAARAGKPAKLFAVLNARFHEQEAYIVAEAGVPGAITIATNMAGRGTDIKL
                     GGSLEMRVMHETAGITDEVEKAKKIELIKADVERFREIVLKAEDVVEIEPAKGAKPAK
                     TATRPGGLYIIGSERHESRRIDNQLRGRSGRQGDPGRSKFFLSLEDDLMRIFGSDRLD
                     SMLTRLGLKEGEAIIHPWINKALEKAQQKVEARNFDIRKNLLKFDNVQNDQRKVIFDQ
                     RVDLMKDESVAETVADMRRAFVEDVVTKHVPEHAYAEQWHVAELKEELKRVLDIDLPV
                     DAWAKEEGIADEELLSRIEQRVDEHMAAKVAQWGPDVMRYVEKTILLQTLDHLWREHL
                     VMLDHLRQVIGLRGYGQRDPLQEYKSEAFSLFEAMIAHLREAVTAQLMRVEIVPPEEQ
                     PALPHMEAHKLDPNTGEDELAFASASLVPMATADRDPKNPASWGKIGRNEDCPCGSGK
                     KYKHCHGKYA"
     gene            complement(2779..4278)
                     /locus_tag="VN968_25425"
     CDS             complement(2779..4278)
                     /locus_tag="VN968_25425"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HXL32604.1"
                     /translation="MTYRSLVRALVTSVALTAGVLLAGCNSDEISLANNAKANQPVPP
                     KLVADMAEKNMDLQSPMLVRLFKQEAELEVWKQDRNGRFALLKTYPICRWSGDLGPKV
                     REGDRQAPEGFYSISPAQMNPQSAYYLSFNTGYPNAFDKALGRSGSQLMVHGDCSSRG
                     CYAMTDEQIAEIYSLGRESFFGGQRAFQLQAYPFKMTPLNMARHRNNPNMPFWKMIKE
                     GYDHFEVTRQEPKVDFCEKKYVFDAAKPPDAAKDPVFEASAKCPAYVIPDEVTRAVAE
                     KQQADQAETAKLITQGTPVARINTGIDGGMNKVFASKLPDGSTGLSEGGDGQGLSLVA
                     LSRAPGTIPSHVNPPRGPVTSPDEPLAASLAQAQASTPATRVASAAPNAQSDGFFSSL
                     ARKVGLGGAADTTASSPPPPVPAKPKIVEAKRSDQVAAAPKTATATPKPDTTKPDTTK
                     QAAARPPQKPALADPAPAPAAAPAKDGMVAGAQPIVSPNSFDSRFQSFR"
BASE COUNT          899 a         1396 c         1464 g          807 t
ORIGIN      
        1 cacgctcggc cagcgccatt tcgacgtgca gatgatcggc ggcatggtgc tgcacgaagg
       61 cgatatcgcc gagatgaaga ccggcgaagg caagacgctg gtcgcgacgc tcgcggtcta
      121 cctcaacgcg ctggccggca aaggcgtcca tgtcgtcacc gtcaacgact acctcgcccg
      181 gcgcgactcc gaatggatgg ggcagatcta caatttcctc ggcctcagcg tcggtgtgat
      241 cgtccacggc ctcgacgacg ccgagcgcaa gaattcctat ggctgcgaca tcacctacgg
      301 caccaacaac gaatacggtt ttgactatct gcgcgacaac atgaagtacc ggctcgagga
      361 catggtccag cgcggtcatt tctacgccat cgtcgacgag gtcgactcga tcctgatcga
      421 cgaggcgcgc acgccgctga tcatctccgg tccgctcgac gaccgttcgg aattctacaa
      481 caccatcgac accttctttc ccaagctcga caagaccgat tacgatgtcg acgagaagca
      541 gcgcacggtg acgctgaccg aagccggcat ggaaaagatc gagaccctgc tgcgcgacgc
      601 cggccagctc aagggcgatt cgctctacga cgtcgagaac gtctcggtcg tccaccacat
      661 caaccaggcg ctacgcgcgc attcgctgtt ccagcgcgac aaggactaca tcgtccgcga
      721 cgatgaggtg atcatcatcg acgagttcac cgggcgcatg atgcagggcc ggcgctattc
      781 ggaaggcctg caccaggcgc tggaagccaa ggagcacgtc caggtccagc cggaaaacca
      841 gacgctggcc tcgatcacgt tccagaacta tttccggatg tacgaaaagc tctccggcat
      901 gaccggcacg gcgttgaccg aagccgacga actgttcgac atctacaagc tcgaggtcgt
      961 ggaaattccg accaatgtga tggtggcgcg gctcgacgaa gacgacgagg tctatcgcac
     1021 ccagaccgag aaatacgccg cgatcctggc cgaggtggaa cgcgccaatg tccggctgca
     1081 gccggtgctg gtcggcaccg cctcgatcga aaagtccgaa gtcctggccg aatacctgaa
     1141 aaagaacggc tacaaacaga tcgatttcgg caacgaaaac gcgatggaga agctctacgc
     1201 cgcggcccgc gccggcaaac ccgcaaagct gttcgcggtg ctgaatgcgc gcttccacga
     1261 acaggaagcc tacatcgtcg cggaggccgg cgtgcccggc gcgatcacga tcgcgaccaa
     1321 catggccggc cgcggtaccg acatcaagct cggcggctcg cttgaaatgc gggtcatgca
     1381 cgagaccgca ggcatcaccg acgaggtcga gaaggccaag aagatcgagc ttatcaaggc
     1441 cgacgtcgag cgttttcgcg agatcgtgct gaaggcggaa gacgtggtcg agatcgagcc
     1501 tgcgaagggc gcgaagccgg cgaagaccgc gacccggccg ggcggcctct acatcatcgg
     1561 ctccgagcgc cacgaatcgc ggcgcatcga caatcagctg cgcggccggt ccggacgcca
     1621 gggcgatccc gggcgctcga aattcttcct gtcgctggaa gacgatctga tgcggatctt
     1681 cggctccgac cggctcgaca gcatgctgac gcggctcggc ctcaaggaag gcgaggccat
     1741 catccatccc tggatcaaca aggcgctgga gaaggcccag cagaaggtcg aggcccgcaa
     1801 cttcgacatc cgcaagaatc tcctgaagtt cgacaacgtc cagaacgacc agcgcaaggt
     1861 gatcttcgac cagcgcgtcg acctgatgaa ggacgaaagc gtcgccgaga cggtcgccga
     1921 catgcggcgc gcctttgtcg aggatgtcgt caccaaacac gtgcccgaac acgcctatgc
     1981 cgagcagtgg catgtcgccg agctgaagga agagttgaag cgggtgctcg acatcgatct
     2041 gccggtcgac gcgtgggcca aggaagaagg catcgccgac gaggaactgc tgtcgcgcat
     2101 cgagcagcgc gtcgatgagc acatggcggc caaggtggcg cagtggggcc ccgacgtgat
     2161 gcgttacgtc gagaagacca tcctgctgca gacgctggac catctctggc gcgagcatct
     2221 ggtgatgctc gatcatctgc gtcaggtgat cggcttgcgc ggctatggcc agcgcgatcc
     2281 gttgcaggag tacaagtcgg aagcgttcag cctgttcgag gcgatgatcg cgcatctgcg
     2341 cgaggcggtg acggcgcaac tgatgcgcgt cgagatcgtg ccgccggaag agcagccggc
     2401 cctgcctcac atggaagccc acaagctcga tccgaacacc ggcgaggacg agctcgcctt
     2461 cgccagcgcc tcgctggtgc ccatggccac cgccgaccgc gacccgaaga atccggcgag
     2521 ctggggcaag atcggccgca acgaggattg tccctgcggc tcaggcaaga agtacaagca
     2581 ctgccacggc aagtatgcgt gaggcgggct ttgggttatg cctgattttg caccgacgtc
     2641 agcatccgtg aacgaagcgc aggggtgggg caaggcgctt gtcgcgccgt gcccaccata
     2701 atattcaaca gcgatgatgg tctgtaacgg cgggctcgct tcgcttggcc cgcccaccgg
     2761 ttctgcgcga gttgcggcct acctgaacga ctggaaccgg ctgtcgaacg aattcggcga
     2821 cacgatcggc tgtgcgccgg cgaccatgcc gtctttggcg ggcgctgctg cgggtgcggg
     2881 tgcggggtca gcgagcgccg gcttctgggg tggccgggcg gcggcctgct tcgtggtatc
     2941 gggcttcgta gtgtcgggct tcggcgtcgc ggtggcggtc tttggcgccg cggcaacctg
     3001 gtcgctgcgc ttggcctcga cgattttcgg ttttgccgga accggtggcg gcgggctcga
     3061 cgccgtggtg tcggcagcgc cgccgaggcc aaccttgcgg gcgaggctgc taaagaaacc
     3121 gtcggattgc gcattcggtg ccgcggatgc gacgcgcgtc gcaggtgtgg aggcctgagc
     3181 ctgcgccagg gaggccgcca gcggctcgtc cggggacgta accggcccgc gcggcggatt
     3241 gacatgggag ggaatcgtgc ccggcgcgcg ggaaagcgcg accagcgaca ggccctgtcc
     3301 gtcgccgcct tccgacaggc cggtactgcc atcgggcagc ttggaggcga acaccttgtt
     3361 catgccgccg tcgatgccgg tgttgattcg ggccaccggc gttccctggg taatcagctt
     3421 ggcggtctcg gcctgatccg cctgctgctt ctccgccacg gcgcgggtga cttcatccgg
     3481 gatcacatag gccgggcatt tggcggaggc ctcgaatacc ggatccttgg cggcgtccgg
     3541 cggcttcgcg gcgtcgaaca catatttctt ttcgcagaaa tcgacctttg gctcctgccg
     3601 cgtcacctcg aaatgatcgt agccttcctt gatcattttc cagaacggca tgttcggatt
     3661 gttgcgatgc ctggccatgt tcagcggcgt catcttgaac ggataggcct gcagctggaa
     3721 ggcgcgctgg ccgccgaaga aggattcgcg ccccaatgaa tagatttccg cgatttgttc
     3781 gtcggtcatg gcgtagcagc cgcgcgacga gcagtcgcca tgcaccatca gctgcgagcc
     3841 tgagcgtccg agcgctttgt cgaaggcgtt gggatagccg gtgttgaacg aaagataata
     3901 cgccgactgc ggattcatct gtgccggtga gatcgaatag aagccttccg gcgcctggcg
     3961 atcaccttcg cggaccttgg gtccgaggtc acccgaccag cggcagatcg ggtaggtttt
     4021 cagaagcgcg aagcggccgt tgcgatcctg cttccagacc tcgagttcgg cttcctgctt
     4081 gaacagccgg accagcatcg gcgattgcag gtccatgttt ttttccgcca tatcggcgac
     4141 gagctttggc ggcaccggct ggttggcctt ggcgttattc gccagcgaga tttcgtcgct
     4201 gttgcagccg gccagcagca caccagccgt gagcgcgaca gatgtgacga gcgcgcgaac
     4261 cagcgagcga tatgtcaatg cagagctcca caccctacgg gcaattcgcg caccccaatc
     4321 atagcgttca tgccctgaag ccattgacta aaatactatc ctttggggcc cttgctcgca
     4381 agccggtcga gccgtcacgg cggttacgga tcagggatgg tcaacagacg ttaaatatcg
     4441 gtgtctgggc ctaattgggg ccgagaccgg cgattttcga aaaggccgag tttttacggg
     4501 caaaacggtc gttttgacaa ccttttcccg caaaaacggc cggtttcagc ccagtttgcg
     4561 gccgat
//
LOCUS       DATNOI010000628        15694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_742346,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000628 DATNOI010000000
VERSION     DATNOI010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 15694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15694
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742346"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1221
                     /locus_tag="VN968_25430"
     CDS             196..1221
                     /locus_tag="VN968_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HXL32605.1"
                     /translation="MSQSAKRVVLVSRPVGEPKGSDFRVEDYALPTPGEGQVLLRTIW
                     LSLDPYMRGRMSDGPSYAAPVPIGGVMEGGTVCEVIASNNPGFAEGDIVLSRAGWQTH
                     ALSDGKDLRKVDPSLGPVSAAVGVLGMPGMTAYTGLLDIGKPQPGETVVVAAASGAVG
                     SAVGQIARIKGARAVGIAGGKDKCDYVKKELGFDDCLDHRDPDLAAKLKEACPKGIDV
                     YFENVGGKVFEAVFPLLNAFARVPVCGLIAHYNDTEATAPKWASSMMRAILTKRLNIR
                     GFIVSDFAARHADFLKDMSGWVREGKVRHREFVTEGLDSAPAAFMGLLKGANFGKQLV
                     RVGPDRA"
     gene            1434..2042
                     /locus_tag="VN968_25435"
     CDS             1434..2042
                     /locus_tag="VN968_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL32606.1"
                     /translation="MIKLVIKLGTLATFLVAMNTASSLTPAFAAGGGGGGGGDASSAM
                     DPGPIYPPSSSPKRSGTKTTHKVKKASKQSSIDDSAFSQGYRAAYATIYDRNDYAAAI
                     EQLKALGHDDHPNVANLIGYSYRKLGDYKLSQVWYERALKADPNHVLTWQYYGLWQIE
                     QGNRDQAQYHLSRIAAICGTDCEEYRSLAAALEKPPGTGLVY"
     gene            complement(2086..2442)
                     /locus_tag="VN968_25440"
     CDS             complement(2086..2442)
                     /locus_tag="VN968_25440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32607.1"
                     /translation="MVEDKRRDQEAALAAGEALLGQGSIGHNHFWFRRYAIERALLLE
                     DLNEATRQADTLLARMSAEPLAYASVVAERCHYLVRRGLGEATEMDEDKHRLLRATAT
                     ETGWRIDALGSALRQM"
     gene            complement(2769..4844)
                     /locus_tag="VN968_25445"
     CDS             complement(2769..4844)
                     /locus_tag="VN968_25445"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXL32608.1"
                     /translation="MKCELENRAGRKFCASCGTALPVACESCGFPNEATEHYCGGCGH
                     ALVGEKIAAVEAGRNVDPGGDRRPVTVLFCDLVGYTRLSSALDAEDVHAMLERFFALV
                     DATVDRFGGTIDKHIGDAAMALFGAPLARGNDAERAVRAALEIQASVPKLASGLPSAL
                     AVHIGIATGEVIASSVGSQHHRGYTVTGEAANIAARLLEKAVSGETLVSGDVYQATNH
                     VVSYQPLGPLALKGVGHSVEVWRPMGIRSSTPQTHALVGRRSELGQFRAVLDACVNGA
                     TGTAVLIRGEAGIGKTRLMDELQSIAAASGMSCTAGFVLDFGTARGHGAVRTVVAGLL
                     GLGSEVTPDIAESAIEEILRDGRLQADDALYLRDLLEMSPPETMRKHYEAIDAAARTH
                     GKERVVAALTKASADSRPLLVTVEDVHWADPETLSLLAAITRATAMSRTVLVMTTRIE
                     GDPLDAHWRSLAGGGTLVTVDLSPLSPADARSIARRFIDVAAFADQCVERAGGNPLFL
                     EQLLRGAADLTDGRLPASIQSVVLARTDLLSAQDRRAIQAASVLGQHFNRAHLRALLQ
                     EPQYDCDTLVRNVLIRPTRGGLQFAHALVRDGVYGSLTNARKRELHGAAAVIFIDDPV
                     LRAEHLDRASDPQAARAYLAAANAQAILFRQDQAIALAARGLVLATKAHDTFQPRHDG
                     ALPHLHLQV"
     gene            5307..7661
                     /locus_tag="VN968_25450"
     CDS             5307..7661
                     /locus_tag="VN968_25450"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32609.1"
                     /translation="MMNAHNPNRSLIDIFCLSLTGLLLVYSTIAVYGLFALESTHATE
                     LAAGLLRIALGLLALGGLLRWGWLAEPLERHVATMGETFEADFANLSAKTRIRSIVLV
                     TMLSLLLELVLIRWLASMFPVYSFYKNFTMLACFLGLGAGYAVAERQPCAPAIVLPML
                     ALFVGVITLLRYDTGAAYLIFSVGWLSTFDLPVYFLLGASFIVCACICYPVGQLCGKL
                     LHSSPSLNAYSFNLAGSILGVIALFVMSLYWLPPVIWFAVTGAIMLLVVLPRDKFLPV
                     GIASFCVLLAILAWPVQPETQRIYSPYQLLERTAKADGLMQILSGGSYYQKVYNFADS
                     KRGYESDADRYVRAYYEFPYNFRKNPERVAIVGAGSGNDAATALRMGAAHVDAIEIDP
                     AIAFLGTKYHPERPYDDPRVNLSINDARNFFRTANQQYDLIVYGVLDSHTALSHASNL
                     RVDSYVYTREGIAEAFRLLKPDGVLSISFTLVNDALGFKLSKILRDLPGAGKPLAVRV
                     LYDNSRTTAFIVKKGQAVAMPDANAFASIGFTDVSDYFAQPYPESAIPTDDWPFFYMV
                     ERAYPVTYMIALGMVLLLSYLFVRKTIGFSDPIDRSYLPFFFLGSGFMLVETKAITEL
                     GLNLGGTWFVIAAAIMLVLVMAFLANLIVTRTTLRLIGPAYVGLFASLLVGYVLATSH
                     GQWVFGPPLMQLATSCVVLTVPLFFAGIIFSSLIGEARINISTAFAYNLMGALFGGVM
                     EYNSMYFGFAFLYLLALGFYSLAWAFSRKSTSLIRTGQAAPVRP"
     gene            complement(7709..8011)
                     /locus_tag="VN968_25455"
     CDS             complement(7709..8011)
                     /locus_tag="VN968_25455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32610.1"
                     /translation="MIRTAITLAMLLCATAARALTVIEKLCIVTAAQDLLHVPGLEIK
                     ASRITTAPADYLGDQDMKYFGAEIDVVAAGQTMTFAFACGTSPRQQIFTRSIGIVR"
     gene            8234..8752
                     /locus_tag="VN968_25460"
     CDS             8234..8752
                     /locus_tag="VN968_25460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32611.1"
                     /translation="MKLLLAGFISLLAASAMADPANKPTNGTELRSAQAEVSKPDNVP
                     VGGCMPIGLTARGDLVFPMQCRELIERERGPVPEQQLHVPLQSSRQEQPKATTAEKDV
                     AATGQTPASLIPIGQAASDQTQTGQLQAGEFLTGQTQKRAHKRLSRAERRAKQGLPPE
                     PAPQVTGSIVRN"
     gene            complement(8849..9277)
                     /locus_tag="VN968_25465"
     CDS             complement(8849..9277)
                     /locus_tag="VN968_25465"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32612.1"
                     /translation="MKPIPRRALLAIVAGFVINAGLQHAAALSEVNKNTQRRLSISVP
                     DSSDDPTPDGPSWHNITQIVAEDLRASSRFVLIEPDSPIVERIDAVPRFDEWRSIHTE
                     WLLTGRVTQPDRRLKVEFRPWNVITGQQALGQQYFLQPEQ"
     gene            complement(9293..10132)
                     /locus_tag="VN968_25470"
     CDS             complement(9293..10132)
                     /locus_tag="VN968_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXL32613.1"
                     /translation="MPEFRLTQISDTHLARRLPSLTQNFHRLSEYIDATRPDLVVNSG
                     DVAFDGPTSRDDLEFARELHHALPVACRYLPGNHDIGDNPTAVGPVPPQLATEQDRQT
                     FLEIFGEDRWRFEAAGWCFIGLNSLVMNAGIASEAEQFDWLASELSSTKGKPVALFLH
                     KPLYLNTPDDPELPATAIRYVPMPARSRLIDMLRPVDLRLVASGHVHQRRDFTFAHIR
                     QVWAPSAGFIISDQRQEVIGIKEVGLVEYRFQPDSFEVRHIRAPGQVDVDLDSLIGAA
                     SKS"
     gene            complement(10336..11049)
                     /locus_tag="VN968_25475"
     CDS             complement(10336..11049)
                     /locus_tag="VN968_25475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acriflavin resistance protein"
                     /protein_id="HXL32614.1"
                     /translation="MDIIPPIPESAAHAAGPPGYPVTSLLKMVTDNSGLFADDSPDGW
                     LGIAMSGLAGVATALAVAIVLTVYFRSGYRSARDIFKHGLAAAAVLALLAFAATDMRH
                     AALAYLGINPSKPAVEFEIRLPKAAVSTVANSQIELHTDRNQTLAQVQAALGSDDDGR
                     SIIRGSVPLEFRTTDRVVILNLPGQPQCQFKLRLAASPSRSDQFGPWHLADRVASAVG
                     GEETRTEQNDAFAIRYRVL"
     gene            11349..11771
                     /locus_tag="VN968_25480"
     CDS             11349..11771
                     /locus_tag="VN968_25480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HXL32615.1"
                     /translation="MSVQMVLLPVFVLVGLAFALLFAMATSRGHALSGRETSPKDIAL
                     GQPNWPTRTTQIGNCFSNQFELPVLFYIVIALALPLRRADLVIVLLSWVFVVTRFAHA
                     GIFVTSNNVQRRSMAWLAGVLVLFAMWLYFALKVLLLI"
     gene            11841..13181
                     /locus_tag="VN968_25485"
     CDS             11841..13181
                     /locus_tag="VN968_25485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HXL32616.1"
                     /translation="MTPAARLSAAIELIETIDAQRVPAAKALKEWGTAHRYAGSGDRA
                     AISGLIWDVLRRRASSAWIMDDDTPRARVLGMLKVERAMDADAIAALCDGGRFAPEPL
                     SEAERAALTSRSLAGAPAPIAGDYPEWLDGYLAQVFGDDRVAEATAMASRAPLDLRVN
                     TLKAKVEKILGSMAHLGAKPTPWSPIGLRIVLGADARNPGIHAEEDFIKGAIEVQDEG
                     SQLAALLSAAKPGEQVIDLCAGAGGKTLALAAMMQGKGRLIATDHDKRQLAPIYERLS
                     RAGVHNCDIRTPKGEGDTLSDIHASADLVLIDAPCTGTGTWRRNPDAKWRMRPGALEV
                     RLKDQVTVLDRAAALTKAGGRIAYITCSVLPPENGEQIRAFVARHPDFVIVPPLQTAT
                     VLWDKAEDFAAATLQSAEGLLMTPRRTGTDGFFVSVLKKKTSQFSIECGFERRT"
     gene            13181..13876
                     /locus_tag="VN968_25490"
     CDS             13181..13876
                     /locus_tag="VN968_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL32617.1"
                     /translation="MKRQQWIGLLGAVATLVCGSAFADETVNIGGSRAVLIKPDAPRA
                     SVILLPSGDGAINAGDGGDIHSLNVNQLVRTRTAYAARGLAVLVADANTPLDAAVQYM
                     AAIKRPVTVIANSRGTIRAAEGIARGARPNALVLTLGFLSGESGSGQNVMSILGSPAS
                     LPRTLVIHHRQDSCRSTLPAGVEPFVKWSAGRARAAWVSGGTSEGDPCEARAYHDFNG
                     VDGQVVGLAAGFH"
     gene            complement(14031..14411)
                     /locus_tag="VN968_25495"
     CDS             complement(14031..14411)
                     /locus_tag="VN968_25495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXL32618.1"
                     /translation="MQPSHHQAADLAALGREWIAAWNSHDLERVLSLYAEDSEMTSDK
                     IQALGFDASGTLRGKARIGAYWGKALALLPNLRFDLIDTYVSPDSLVVFYQNERGAKI
                     CEYLRLNAEGEIIQGSANHLAQYD"
     gene            14553..>15694
                     /gene="guaA"
                     /locus_tag="VN968_25500"
     CDS             14553..>15694
                     /gene="guaA"
                     /locus_tag="VN968_25500"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HXL32619.1"
                     /translation="MTAAQPARESLPSVASAHDKILIVDFGSQVTQLIARRVREEGVY
                     SEIVPFQKAEAAFIQMKPKAVILSGGPESVHEEGSPRAPQAIFDSGVPVLGICYGQMT
                     MAAQLGGDVEGGHHREFGRADVEVKAASALFDSTWGMGERHPVWMSHGDRITKMPPGF
                     VVAGVSPNAPFAVIQDETRRYYGLMFHPEVVHTPDGAKLIRNFVRKVAGLTGDWTMRA
                     FREEAIEKIRSQIGKGKVICGLSGGVDSAVAAVLIHEAIGDQLTCVFVDHGLLRLNEA
                     ETVVDLFRHHYNIPLVHVDASKLFLGELAGVSDPELKRKTIGRLFIDVFDTEAKKIGG
                     ADFLAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPARMNMKLVEP"
BASE COUNT         2883 a         4780 c         4973 g         3058 t
ORIGIN      
        1 tttggccaac cgagcatcgc caaagcggcc gactgaccaa tccatctctt ccatagcggt
       61 catccctctt gtagaccgca ctgaaaggaa tcagactcga ctccatcttg gcaacagaaa
      121 ttgtgtgcac gccttagccc aatgggagag gggcgcactt ccgctgtcgc tagtttgtcg
      181 ttggaggaaa caaccatgtc gcaatccgca aaacgtgtcg ttctggtttc tcgcccggtc
      241 ggcgaaccca aggggtcgga cttccgcgtc gaggactacg cgctgccgac acccggtgag
      301 ggccaggtcc tgctgcgcac catctggttg tcgctcgatc cctatatgcg cggacggatg
      361 agcgacgggc catcctatgc ggcgccggtg ccgatcggcg gggtaatgga gggcggcacg
      421 gtgtgcgagg tgatcgcctc gaataatccg ggctttgccg agggcgatat cgtcctgtcg
      481 cgggccggct ggcaaaccca cgcgctgtcc gatggcaagg atttgcgcaa ggtcgatccg
      541 agcctcggtc cggtctcggc cgcggtcggc gtgctcggga tgcccggcat gacggcctat
      601 accgggctgc tcgacatcgg caagccgcag cccggcgaaa ccgtcgtcgt tgcggcggcg
      661 tccggcgcgg tcggctcggc ggtcggccag atcgccagga tcaagggcgc gcgtgccgtc
      721 ggcatcgcgg gcggcaagga caaatgcgat tatgtgaaaa aagagctcgg cttcgatgac
      781 tgcctcgatc atcgcgatcc cgatctcgcg gcgaagctga aggaagcctg cccgaaaggc
      841 atcgacgtct atttcgagaa tgtcggaggt aaggtgttcg aagcggtatt cccgctgctc
      901 aatgcgttcg cgcgcgtgcc ggtgtgcggc ctgatcgcgc attacaacga caccgaggca
      961 actgcgccga aatgggcctc gtctatgatg cgcgcgatat tgacaaaacg gctgaatatc
     1021 agaggcttta tcgtcagcga tttcgccgcg cggcatgcgg attttctcaa ggacatgtcg
     1081 ggatgggtgc gcgagggcaa ggtcaggcat cgcgaattcg tcacggaggg tttggacagc
     1141 gcgcccgctg cgttcatggg actgctgaag ggcgccaatt tcggcaagca gctggtgcgc
     1201 gtcgggccgg acagagcgtg agcctccgaa aaaggacagg gtaactgtcg cacacacggt
     1261 cgtgaaatac acgcaactgg cctgcatccg cgtttaatgg cgttccgacg tttaatgacg
     1321 ttccaattcg aagttttgct gaaacaaaaa tccttcaggg gaatgtatcg agcgaagcgg
     1381 agttaacacc tgcagctgga gggatcgtcc gcacacgtag gcaggaggct cgaatgatca
     1441 aactggtgat caagcttggg acgttggcaa catttctggt ggcaatgaat actgcatcct
     1501 cgctgacccc ggcctttgcg gccggaggag gaggtggtgg tggtggtgat gcttcttccg
     1561 cgatggaccc gggtcctatt tatccgcctt cgtcatcccc gaagcggtcg ggcaccaaaa
     1621 ccacccacaa ggtcaagaag gccagcaagc agtccagcat cgacgactcg gcatttagcc
     1681 agggttatcg cgcagcctat gcgacgatct acgatcgcaa cgactacgcc gccgctatcg
     1741 agcagttgaa ggcgctcggc catgacgatc acccgaacgt ggccaatctg atcggttatt
     1801 cctatcgaaa acttggcgac tacaagcttt cgcaggtctg gtacgaacgc gcgctgaaag
     1861 ccgatccgaa ccacgtgctg acgtggcagt attacggtct gtggcagatt gagcagggta
     1921 accgcgatca ggcgcaatat cacctgagcc ggatcgctgc gatttgcggg accgactgcg
     1981 aggagtacag gtcattggcg gccgcactcg aaaagccacc cggcaccggt ctcgtttatt
     2041 gagccggcgc gtccaaagaa gcctgccgta gccggcgcaa tgcagttaca tctgacgcaa
     2101 tgcgctaccg agtgcgtcga tgcgccagcc ggtctcggtg gcggtcgcgc ggagaagcct
     2161 atgcttatct tcgtccatct cggtggcttc gcccagtccg cgacggacca ggtaatggca
     2221 gcgctcggcc acaacggatg catatgcgag cggttcagcg gacatgcggg caagcagcgt
     2281 gtcagcttgc ctggtggctt cgttcaaatc ctcgagcagt agcgcgcgtt caatggcgta
     2341 acggcggaac caaaagtgat tatggccgat ggatccctgt ccaagcaagg cttctccggc
     2401 ggccagagcc gcctcctgat ccctccgctt gtcctcgacc aacgcgagga taccgtagag
     2461 tatgggcccg gcaaacccgt gtctcctcgg aaatctcctg cccctttcgc gccagcgcca
     2521 gggcttccgc tctcagtcct tttgaaaggg cggcctcggc ggtgtgccca aggataacgg
     2581 cctcgtagcg ccgcgccttg agcatccttg cctgctctaa cgccctggct tgaagatcat
     2641 ctgcatccgc atagcgtccg gctgccgtca gacagagacc catcgattgg gtcgcaaaca
     2701 tctccgcatg cccgttgccg atcctgatcg cggcttcccg gcccaagcgc atatcctcca
     2761 gtcccaggtc aaacttgcag gtgtaaatgc ggcaatgccc catcatgacg cggttggaat
     2821 gtgtcgtgtg ccttggtggc gagcaccagg ccgcgtgccg caagcgctat cgcctggtcc
     2881 tggcggaaca ggattgcctg cgcattggcc gccgcaagat aggcgcgcgc tgcctgggga
     2941 tcgctcgcac ggtccagatg ctcagctcgc agcaccggat catcgataaa gatcacggcc
     3001 gcagcaccgt gcaactcccg cttccgtgcg ttggtgagcg atccgtagac gccgtcgcgt
     3061 accaacgcat gagcgaattg tagaccgccc cgcgttggac gtatcaggac attgcgtacc
     3121 aacgtgtcac aatcgtactg cggctcctgc agcagcgccc tgaggtgggc acggttgaag
     3181 tgctggccga gcacggaggc tgcctggatg gcgcggcggt cctgggcaga tagcagatcc
     3241 gtacgggcca acacgacgct ttgaatggat gccggcagcc gtccgtccgt gagatcggcg
     3301 gcgcctctta gcaattgctc caggaacagc ggattgccgc cggcacgctc gacgcactga
     3361 tcggcgaagg ccgcgacgtc gatgaagcgt cgcgcgatcg accgcgcgtc cgcaggcgac
     3421 agcggagaaa ggtctacggt gaccagcgtt ccaccgccgg cgaggctgcg ccaatgcgca
     3481 tcgagcggat cgccttcgat gcgagtggtc atgacgagta ccgtccggct catcgctgta
     3541 gcgcgcgtga tggcggcaag aaggctcagc gtttccggat ctgcccaatg cacgtcctcg
     3601 acggtgacga gaagcggccg actgtctgcc gaagccttgg tcagagcggc aaccacgcgc
     3661 tccttgccat gggtccgtgc agctgcgtcg atcgcctcat agtgcttgcg catggtttcg
     3721 ggcggagaca tctcaagaag gtcgcgcaga tagagcgcgt catctgcctg cagacgtccg
     3781 tctcgcaaaa tttcctcgat cgcgctctcg gcaatgtctg gcgtgacctc ggagccaagc
     3841 cccagaagcc cggcaaccac cgtgcgcacg gcgccgtgac cgcgcgcggt accgaaatcg
     3901 aggacgaatc cggcggtgca cgacatgcct gacgccgcgg cgatcgactg caactcgtcc
     3961 atcagacggg tcttgccgat cccggcctcg ccgcggatca gcacggcggt accggtggcc
     4021 ccgttgacgc aggcgtccag cacggcgcgg aactgcccga gctcggatcg acggccgact
     4081 aatgcatggg tctgcggggt cgagcttctg attcccatcg gtcgccagac ctcgacggaa
     4141 tgcccgacgc ccttcagcgc cagcggtccc aacggctggt aggaaacgac gtgattggtt
     4201 gcctgataga catcgccgga aaccagcgtt tcgccgctga ccgccttctc caaaagccgc
     4261 gcggcaatgt tggcggcttc acccgtgacg gtatagccgc gatggtgctg gcttcccacc
     4321 gaactcgcga tgacttcacc ggtcgctata ccgatatgaa cggccagggc ggaaggcaga
     4381 ccggacgcga gcttcggaac ggatgcctgg atctccaagg cggcccgcac ggcgcgctcg
     4441 gcatcgttgc cgcgcgcgag cggcgctccg aacagcgcca ttgccgcgtc gccgatgtgc
     4501 ttgtcgatcg tcccgccaaa gcggtcgacg gtcgcatcga cgagcgcaaa gaagcgttcc
     4561 agcatggcat ggacatcttc ggcatccagc gccgaagaga ggcgggtata gccgaccagg
     4621 tcgcagaaca atacggtaac cggacgccga tctcccccgg gatcgacgtt ccgtccggct
     4681 tcgaccgccg cgatcttttc gccgacaagg gcatgaccgc agccgccgca ataatgttcg
     4741 gtcgcctcgt tcggaaagcc gcagctctcg caggctactg gcaatgctgt gccgcaagac
     4801 gcacaaaatt tgcgtcccgc ccggttctcc aattcgcact tcatgcaccg cacagcgccg
     4861 gactccgtag tctgggattc cgttgtctgg aatgatgagc cggagtgagg gacaccgcaa
     4921 ggagatcgtc gccggccgtg tgctaccaac gcatgtccac cgatgacggc tgaacggatc
     4981 cggcacggat acccggccag tgccggccat catccgcatc ggctggcggg cgggcggaaa
     5041 acagcggagg cgatgcgcgt gcaatcgatc attcggtgat tgcgatggcc ggcacccgga
     5101 tgcaaccgga cgttaatacg tcgataacat cgtcgagcag acggtcgcgc cgtctcctcg
     5161 ccggtaatgg tctcttaagc aaacccgccc taacgtccgg cgcacgcctg acgcgccgtg
     5221 aataccctgg ccacgagcaa atcgtggcgc tcacgatcgg ctgacgcgtg agcgcaccat
     5281 ctcaccaacg ctcccggccc cagcccatga tgaatgccca caatcccaat cgcagcctca
     5341 ttgatatctt ttgcctgtcc ctgaccggct tgctgctcgt ctattcgacg atcgccgttt
     5401 acgggctgtt tgcgctcgaa agcacccatg cgacggagct ggctgccggc ttgctccgga
     5461 tcgcactcgg gctgcttgcg ctcggcggcc tgctgcgctg gggctggctc gccgaaccgc
     5521 tggagcgcca cgtcgcaacc atgggcgaaa cgttcgaagc cgattttgcg aatctgagcg
     5581 cgaagacgag gatcaggtcg attgtcctgg tgacgatgct cagcctgctc ctggagctgg
     5641 tgctgatccg ctggctggca tcgatgtttc cggtctattc cttctacaag aatttcacca
     5701 tgctggcttg cttcctgggt ctcggtgccg gttacgcggt cgccgaacgg cagccctgcg
     5761 ctccggcgat cgtgttgccg atgctggcgc tgttcgtcgg ggtgatcacg cttttgcgct
     5821 acgataccgg cgcggcgtat ctgatatttt ccgtcggctg gttgagcacg ttcgacctgc
     5881 cggtctactt cctgcttggg gcaagcttca tcgtctgcgc atgcatctgc tatccggtgg
     5941 gccagctttg cggcaaattg ctgcacagct cgccttcgct gaacgcctac agcttcaacc
     6001 tcgcgggcag cattctcggg gtcatcgccc tgttcgtgat gagcctctac tggctgccgc
     6061 cggtgatctg gttcgccgtg acgggcgcga tcatgctgct cgtggttctt ccgcgcgaca
     6121 aattcctgcc ggtcgggatc gcaagctttt gcgtcctgct ggcgatcctg gcctggcccg
     6181 tgcaacccga gacgcagcgg atctactctc cgtaccagtt gctggagcga acggccaagg
     6241 ctgacggatt gatgcagatc ctgtccggcg gaagctacta tcagaaggtc tacaatttcg
     6301 cggacagcaa gcgtggatac gagtccgacg cggatcgcta tgttcgggcc tattacgagt
     6361 ttccctacaa tttccggaag aacccggaac gcgtcgccat cgtcggcgcg ggcagcggca
     6421 acgatgccgc gaccgcgttg cggatgggtg ctgcccatgt cgatgccatc gagatcgatc
     6481 ccgccatcgc gtttctcggc accaaatatc atcccgagcg cccctacgac gacccccgcg
     6541 tcaacctctc gatcaacgat gcacgaaact tcttccgcac cgcaaatcag caatacgatc
     6601 tgatcgtcta tggcgtgctg gattcgcaca ccgcgttgag ccatgcgtcc aatctgcgcg
     6661 tcgactccta tgtctatacg cgggaaggaa tcgccgaagc cttcaggctg ctcaagccgg
     6721 acggcgtgct ctccatctcg tttaccctgg tcaacgatgc cttgggcttc aagctgtcga
     6781 agattctccg ggacctgccg ggtgccggca agccgctggc ggtccgggtg ttgtatgaca
     6841 attcgcgaac caccgcattc atcgtcaaga aagggcaggc cgtggcgatg ccggatgcga
     6901 acgccttcgc gtccatcggc ttcaccgatg tctcggatta tttcgcgcaa ccctatccgg
     6961 agtccgccat tccgaccgac gactggccgt ttttctacat ggtcgagcgc gcctatcccg
     7021 tcacctacat gatcgcactc gggatggttc tgcttctcag ctatctgttc gtgcgcaaga
     7081 ccatcgggtt ttccgatccg atcgaccgca gctatttgcc gtttttcttc ctgggctccg
     7141 gcttcatgct ggtggaaacc aaggcgatta ccgagctcgg gttaaacctt ggcgggacct
     7201 ggttcgtcat cgccgcggcc atcatgctgg tgttggtgat ggcgttcctg gccaatctga
     7261 tcgtgacgcg aaccacgctc aggctgatcg gaccggccta tgtcgggttg ttcgcaagcc
     7321 tgttggttgg atacgttctg gcgaccagtc acggccaatg ggtgttcggg ccgcccttga
     7381 tgcaactggc gacaagctgc gtcgtgttga ccgttccgtt gttcttcgcc gggatcatct
     7441 tctccagcct gattggcgaa gccaggatca atatctcgac ggcgttcgcc tacaatctga
     7501 tgggcgccct gttcggcgga gtgatggaat acaattcgat gtatttcgga tttgcatttc
     7561 tctatctgct ggcattggga ttttattctc tggcctgggc gttttcacgg aagtccacct
     7621 cgctcattcg aaccgggcag gccgcaccgg tcaggccata accggaagtt gctgccgcca
     7681 tttcaaggac gtcgcgccgg cctttgatct agcgcactat cccgatcgag cgcgtaaaga
     7741 tctgctgccg cggcgaggtt ccgcaggcaa acgcaaaggt catggtttgg ccggcggcca
     7801 cgacgtcgat ctcggcacca aaatatttca tgtcctggtc gccgagatag tcggccggcg
     7861 ccgtcgtaat ccgactggct ttgatctcaa gaccggggac gtggagcaaa tcctgcgccg
     7921 cggtcacgat gcacaatttt tcgatcacgg ttagggcccg cgccgcggtt gcgcatagca
     7981 gcatcgccaa cgtgattgca gttctgatca ttgccgtctc cgagtgcggt ccgctggagg
     8041 gagcgtcgag gcgccggccc ggccgggcgc tggcgttcgc tcgcatccgc acagcgcgat
     8101 ataattacat cacgtgtgga ctcacgtgat ggtgccaggt ccaaatgctt atatttcggg
     8161 cattcgacga ttgatatttc gagcagcgcc gtggtagtgc aaaacctttc gtgtacgcgt
     8221 aaaggacttt ctgatgaagc tcttgctcgc aggctttatc tcgctgctcg ccgcctcggc
     8281 gatggcagac ccggctaaca agccgaccaa tggaacggaa ttgcgcagcg cacaagcaga
     8341 ggtttccaag ccggacaatg tgcccgtcgg cgggtgcatg ccgatcggcc tgaccgcccg
     8401 gggtgacctt gtttttccga tgcagtgccg cgaattgatc gagcgagagc gcggccccgt
     8461 tccggagcaa cagctgcatg tacccctgca gagttctcga caggagcagc cgaaggcaac
     8521 tacggcagag aaggatgttg ctgcaacggg gcagacgcca gcgagtctga ttccgatcgg
     8581 gcaagctgca tcagatcaaa ctcagacagg tcagcttcaa gccggtgagt ttctaacggg
     8641 ccaaactcaa aaacgcgcgc acaagagact ttccagggcc gagcgacgcg ccaagcaagg
     8701 gctcccgccg gaacccgcgc cacaggtgac cgggagcatc gtgcggaatt aggacggtcc
     8761 aggagaggtc gctcgatcgg gcgcgcgcac gttattcatc gctcaaccgt ttgaggatct
     8821 cctccgctat gatttgcgga acgttgcgct attgttccgg ttggagaaaa tactgctgtc
     8881 cgagcgcttg ctgacccgtg atcacattcc atgggcgaaa ttccaccttg aggcggcggt
     8941 cgggctgcgt gacgcggccg gtcagcagcc attcggtatg aatgctgcgc cattcatcga
     9001 agcgtggcac ggcgtcgatt ctttccacga tggggctatc gggttcaatc agcacaaatc
     9061 ggcttgatgc ccgcaaatct tcggcgacga tttgcgtgat gttgtgccag ctcgggccgt
     9121 ccggcgtcgg atcatccgag gagtcgggga ccgaaatcga caaccgtcgc tgggtgttct
     9181 tgttcacttc gctcagcgcg gccgcatgct gcaatccagc attgatgacg aagccggcaa
     9241 cgatcgccag tagcgcgcgc cgagggattg gcttcattcc tgtgatggcg cgttagcttt
     9301 tcgaagccgc gccgatcagc gaatccaggt cgacatcgac ctggcccggc gcgcggatgt
     9361 gacggacctc aaaactgtcg ggctggaagc gatactccac caggccgact tccttgatgc
     9421 cgatcacttc ctgccgctgg tcggaaatga tgaagccggc ggaaggcgcc cagacctgcc
     9481 tgatatgggc aaacgtaaaa tcccggcgct ggtgcacgtg gccgcttgcc accagccgca
     9541 gatcgacggg gcgcaacatg tcgatcaggc ggctccgcgc cggcatcgga acgtaacgga
     9601 tcgccgtcgc cggaagctcg ggatcgtccg gagtattcag gtaaagtggt ttgtgaagaa
     9661 acagcgccac cggcttgccc ttggtgctgg agagttctga tgcgagccag tcgaactgtt
     9721 cggcctcgct cgcaattccg gcgttcatga caagcgaatt cagtccgatg aaacaccagc
     9781 cggcggcctc gaaacgccag cggtcttcgc cgaaaatctc aagaaaagtc tgccggtcct
     9841 gctcagtggc cagttgaggc ggaacggggc cgaccgcggt cggattatcg ccgatatcgt
     9901 gattgccggg taaatagcgg caggcaacgg gaagcgcatg gtgcagctcg cgagcaaatt
     9961 cgagatcgtc gcgactggtc ggcccgtcga acgcaacatc gccgctgttg acgacgagat
    10021 cggggcgcgt cgcgtcgatg tattcgctca gccgatgaaa gttttgcgtc aagctcggca
    10081 ggcggcgggc aagatgggtg tcggaaatct gggtcaggcg aaattcaggc atgcaacatt
    10141 ccttccagcc cggattatat aatttgcggg cgtcggaatg gtgacaagat tatgagcgtc
    10201 gcctcgcacc actgccgtca tcatccgcga aagcggatga ttcagtatcc agggcggttg
    10261 tgatggaatc gtgatgccgc ggcgtactgg atgccccgct ttcgcggggc atgacgactt
    10321 atcgtttgtt caggctcaca gcacgcgata gcggatcgcg aaggcgtcgt tctgctcggt
    10381 gcgggtctct tcgcctccga ccgccgaagc gacgcgatcg gccagatgcc atggcccgaa
    10441 ctgatcggag cggcttgggc ttgcggcgag acgcagcttg aactggcatt gcggctgacc
    10501 cggcaggttc aggatcacca cccggtcggt ggtgcggaat tcgagcggga ccgaacccct
    10561 gatgatgctg cggccgtcgt catcggatcc gagcgcggct tgcacctggg ccagcgtctg
    10621 gttccggtcg gtgtgcagtt cgatctggct gttggccacg gtggacacgg cggccttggg
    10681 cagccggatt tcgaactcga ccgcgggttt cgacgggttg atgccgagat aggccaacgc
    10741 ggcatgccgc atgtcggtgg ccgcgaaggc caagagcgcg agcaccgccg cggccgcgag
    10801 gccgtgtttg aagatatcgc gcgcgctgcg atagccgctg cggaaataca cggtcaggac
    10861 gatggccacc gccagggcgg tcgcgacgcc ggccagcccc gacatcgcga taccgagcca
    10921 gccgtcgggg ctgtcgtctg cgaaaagacc gctgttgtcg gtgaccattt tgagcaacga
    10981 ggtcacgggg tagccgggcg gcccggccgc atgggccgcg ctctctggga tcggggggat
    11041 aatgtccatc gcaacctctc gccggtagcg ttgagtgcca aatcgagact gaaatcgctt
    11101 gagccaatgg aagttggtca cgggaatgcg catggtggtt caacccgtcg gcgcaggatt
    11161 tattgtcagg ggggagtgct gccgctatag cgggggcttg gtgcttggaa cgttttcaag
    11221 cgaaggcctg ccccggactt gatccggggt ggagaccggt tcgcgtcaag aaaacgcgtc
    11281 aaatcaacaa tccagagctt cgtttcgatt ctatcgaaac gaggctctgg tccccgcgga
    11341 gaattccgat gtccgttcaa atggttttgc tgccggtctt cgtcctggtg gggctcgctt
    11401 ttgcgctttt gttcgccatg gccacgtcgc gtggccatgc gctctcgggc cgggaaacca
    11461 gccccaagga catcgcgctg gggcagccga actggccgac ccgcaccacc cagatcggca
    11521 attgcttttc caaccagttc gaactgccgg tcctgttcta catcgtgatc gcgctggcgt
    11581 tgccgctgcg ccgtgccgac ctcgtgatcg tgctcctgtc gtgggtgttc gtggtcaccc
    11641 gttttgccca tgccggaatt ttcgtgacct cgaacaacgt ccagcggcgg tcgatggcgt
    11701 ggctcgccgg cgtcttggtg ctgtttgcga tgtggctcta tttcgcgctc aaggtattgc
    11761 tgctgatctg agccaagtgt cctcgacgtg ccctcgaagt gtcgccgaac tggcacctgt
    11821 gctcgaactg aaggttttga atgactcccg cagcgcggct ctccgcggcc atcgaactga
    11881 tcgagaccat cgacgcgcaa cgcgtgccgg cggcgaaggc gctgaaggag tggggcaccg
    11941 cgcatcgcta tgccggctcg ggcgatcgcg ccgcgatctc cggcctgatc tgggatgtgc
    12001 tgcgccggcg ggcatcgagc gcctggatca tggacgacga tacgccgcgc gcgcgcgtgc
    12061 tgggcatgct gaaggtcgag cgcgccatgg atgccgatgc catcgccgcg ctctgcgacg
    12121 gcggccggtt cgcgccggag ccgctgagcg aggccgagcg cgccgcgctg acgtcgcggt
    12181 cgctggccgg tgcgccggcg ccgattgccg gcgattatcc ggaatggctc gatggctatc
    12241 tggcacaggt gtttggcgac gaccgcgtcg cggaggcgac cgcgatggcg agccgggcgc
    12301 cgctcgacct tcgcgtcaac accttgaagg cgaaggtcga gaagattctc ggctcgatgg
    12361 cgcatctcgg cgcaaagccg acgccatggt cgccgatcgg cctgcgcatc gtgctcggcg
    12421 ccgacgcccg caatcccggc attcatgccg aggaagattt tatcaagggc gccattgaag
    12481 tgcaggacga gggctcgcag ttggcggctt tgctgtcggc cgcaaaaccc ggcgagcagg
    12541 tgatcgatct ctgcgccggc gccggcggca agaccctggc gctggcggcg atgatgcagg
    12601 gcaagggccg gctgatcgcc accgatcacg acaagcggca gctcgcgccg atctatgagc
    12661 ggctgtcgcg cgccggcgtc cacaattgcg atatccgcac gccgaagggc gagggcgaca
    12721 ccctgagcga catccatgct tccgccgacc tggtgctgat cgacgcgccc tgcacgggca
    12781 ccggcacctg gcggcgtaat cccgatgcca agtggcggat gcgcccgggt gcgctcgagg
    12841 tccggctaaa ggaccaggtc accgtgctcg accgcgccgc cgcattgaca aaagcgggcg
    12901 ggcggatcgc ctatatcacc tgctcggtgt tgccgccgga aaacggcgag cagatccgcg
    12961 ccttcgtcgc ccgccatccg gattttgtca tcgtgccgcc attgcagacc gcgacagtgc
    13021 tgtgggacaa ggcggaggat ttcgcggcgg ccacgctgca atccgcggag gggcttttga
    13081 tgacgccgcg ccgcaccggc accgatggat tttttgtgtc ggtactgaag aagaagacat
    13141 cgcaattttc aatcgagtgc ggatttgaac ggagaacata atgaagcgac agcaatggat
    13201 cggcttgttg ggggccgtgg caaccctcgt ctgcggttcg gcctttgccg atgagaccgt
    13261 caacatcggc ggctcgcgcg ctgtgttgat aaaaccggat gcgccgcggg cgagcgtgat
    13321 cctgctgccc agcggcgatg gggcgatcaa tgcgggcgac ggcggggata tccactcgct
    13381 caacgtcaac cagctggtcc gcacccgaac cgcctatgcg gcgcgagggc tggcggtgct
    13441 ggtggccgat gcgaataccc ctctcgacgc cgcggttcaa tacatggccg cgatcaagcg
    13501 cccggtcacc gtcatcgcca acagccgcgg cacgatccgc gccgcggagg ggattgcgcg
    13561 cggcgcccgg ccgaatgcgc tggtgctgac gttaggattt ctcagcggcg aatcgggcag
    13621 cggccagaac gtcatgtcga tcctcggctc gccggcgtcg ttgccgcgta cgctcgtgat
    13681 ccatcaccgc caggatagct gcaggtcgac cctgccggcc ggtgtcgagc cgttcgtcaa
    13741 atggtcggcc ggccgtgcgc gggcggcctg ggtgagcggc ggaacaagcg aaggcgatcc
    13801 ctgcgaggcc cgggcctatc acgatttcaa tggcgtcgac ggtcaggtgg tggggctggc
    13861 ggcgggattc cattgatgcg ctgacgcacc atcattccgg gatggtccga aggaccagac
    13921 ctcagatgtg caactgcaca tcggggaatc tcgagattcc gggttcgatg cggagcctgt
    13981 catcgggccg cgctttgcgc ggacccgttg gcatcgcccc ggaatgacgg ctagtcgtat
    14041 tgcgccagat gattggcgga gccctggatg atctcgcctt ccgcgttcag ccgcagatat
    14101 tcgcagattt ttgcgccgcg ctcgttctgg taaaacacaa cgagagagtc ggggctgaca
    14161 taggtatcga tcaggtcgaa acgcagattg ggcaaaagcg ccagtgcttt tccccaatag
    14221 gcgccaatgc gggccttgcc gcgcagcgtt ccgctggcgt caaagcccag agcctggatc
    14281 ttgtccgagg tcatctcgct gtcttcggca tagagcgaaa ggacgcgctc cagatcatgg
    14341 gaattccagg ccgcgatcca ttcccgcccc agggcggcca gatccgcggc ttggtgatgc
    14401 gaaggctgca tggtttcctc ctgacacata taggtcaata gtactgacct atatgaactt
    14461 tgtcgatgct ttcagaagga tgcctgcaga agaatgtggg ccattggttt tgtgcggttg
    14521 cgagcgcagc ccccgtcgcg taattgcttg ccatgacagc agcacagcca gcccgcgagt
    14581 ccttgccttc ggtggcctcg gcgcatgaca agattctgat cgtcgatttc ggcagccagg
    14641 tgacgcaact gatcgcgcgc cgggtgcggg aggagggcgt ctattccgaa atcgtgccgt
    14701 tccagaaggc ggaagcggcg tttattcaga tgaagccgaa ggcggtgatc ctctccggcg
    14761 ggccggaatc ggtgcacgaa gagggctcgc cgcgcgcgcc gcaggcaatc ttcgattccg
    14821 gcgtgcccgt gctcggcatc tgctacggcc agatgaccat ggcggcccag ctcggcggcg
    14881 atgtcgaggg cggccaccac agggaattcg gccgcgccga tgtcgaggtc aaggccgcaa
    14941 gcgcgctgtt cgattcgacc tggggcatgg gcgagcggca tccggtctgg atgagccatg
    15001 gcgaccgcat caccaaaatg ccgccgggct ttgtcgtcgc cggcgtctcg ccgaatgcgc
    15061 cgttcgcggt gatccaggac gagacacgca gatattacgg gctgatgttc catcccgaag
    15121 tggtgcatac gcccgacggc gccaagctga tccgcaactt cgtccgcaag gtcgcagggc
    15181 tcaccggcga ctggaccatg cgcgccttcc gcgaggaggc gatcgaaaag atccgctccc
    15241 agatcggcaa gggcaaggtg atctgcggcc tgtccggcgg cgtcgattcc gccgtcgccg
    15301 cggtgttgat ccacgaggcg atcggcgatc agctgacttg cgtgttcgtc gatcacgggc
    15361 tgctgcggct caacgaggcc gagaccgtgg tcgatctttt caggcaccac tacaacatcc
    15421 cgctggtgca tgtggatgcg tcaaaacttt tcctcggcga actcgccggc gtcagcgatc
    15481 ccgaactgaa gcgcaagacc atcggtcgcc tgttcatcga tgtgttcgat accgaagcca
    15541 agaaaatcgg tggcgcggac tttttggcgc agggcacgct gtatcccgac gtgatcgaaa
    15601 gcgtgtcgtt caccggcggc ccctcggtga cgatcaaatc gcaccacaat gtcggcggct
    15661 tgcccgcgcg catgaacatg aagctggtcg agcc
//
LOCUS       DATNOI010000629         7733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1230227,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000629 DATNOI010000000
VERSION     DATNOI010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230227"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1793)
                     /locus_tag="VN968_25505"
     CDS             complement(<1..1793)
                     /locus_tag="VN968_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXL32620.1"
                     /translation="MLTSIVVSVVKACTRFASLTILVAVILAITAGYYTSRNFIINTD
                     INTLISPDLDWRKRDNQFEKAFDRDRTILAVVDAPTPELTSAAADALAQKLSGDVVHF
                     ESVQPLGSGAFFEKNGLLFLPVAEVSQLTGQFESAAPLIEIMAGDPSIRGMTGALETG
                     LAGVKRGQVKLDGTERPFTLIAQTVENVMKNGSGTFSWRELTSDKPLTDADRRSFIEF
                     KPILDFKALEPGKDATDAIRQAARDLNLAGEYGARVRLTGPVPIANEEFATVQDGAIV
                     NGIGTVLVVLLILWMALHSTKIIFAVFLNLFIGLSITTAVGLWMVGSLNLLSIAFAVL
                     FVGLGVDFGIQFSVRYRSERFKGDDLRLALEKAAERSAVPLSLAAMATAAGFLSFLPT
                     DYKGISELGKIAGVGMLVAFTTSITVLPALLKLLNPPGESEPVGYAFLAPVDYFLEKH
                     RVVIIVGTLLIAVAGLPLLYFLRFDFNPVNLRNPKVESIGTFLDLRSDPNTGANAINV
                     MTNSDADAKKIEERLSKVPEVLRAMSLDSFVPADQPAKLKLIGQGAKILGPALNPDSI
                     DAPPSDQENVESLKGSAENLRRTAGDAKGPGA"
     gene            2272..3198
                     /gene="ispH"
                     /locus_tag="VN968_25510"
                     /gene_synonym="lytB"
     CDS             2272..3198
                     /gene="ispH"
                     /locus_tag="VN968_25510"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HXL32621.1"
                     /translation="MEVYLAQPRGFCAGVVRAIEIVERALEKYGPPVYVRHEIVHNKY
                     VVESLKNKGAIFVEDLSEVPPLAVTVFSAHGVARSVEEEAAVRGLPVLNATCPLVTKV
                     HNQGKRYMSKGRALILIGHAGHPEVEGTMGQVPGPVLLVQNVDDVAELTLPADAPVAY
                     ITQTTLSVDDTKDIIAALQQRFTDIQGPDIRDICYATQNRQSAVRDLSKLVDVILVVG
                     ATNSSNSNRLREIGTEVGVASYLIADGSELNPEWLKHAKAVGITAGASAPEVLVDDVI
                     EALRRIGPVTVSVLPGREENIEFRLPAELTVG"
     gene            3232..4386
                     /gene="hpnH"
                     /locus_tag="VN968_25515"
     CDS             3232..4386
                     /gene="hpnH"
                     /locus_tag="VN968_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HXL32622.1"
                     /translation="MAIPFFKEMRIGGYLMKQKLLGRKRYPLVLMLEPLFRCNLACVG
                     CGKIDYPDAILNRRMSAQECWDAADECGAPMVAIPGGEPLIHKEIGEIVRGLVARKKF
                     VSLCTNALLLEKKLDLFEPSPYLFFSVHLDGLKDHHDKAVSQKGVFDRAVSAIKAAKA
                     KGFTVNVNATIFDGHAAEDIAKFLDFTTELGVGVSMSPGYAYERAPDQEHFLNRTKTK
                     KLFRDVFALGKGKKWNFMHSGLFLDFLAGNQTYECTPWGMPARNIFGWQKPCYLLGEG
                     YTKTFKELMETTDWETYGTGKYEKCADCMAHCGYEPTAADAALQNPLKAMWVALRGVR
                     TTGPMAPEISLDHQRPAQYIFSAEVQKRLSEIRRDEALAAAAKEKASTAA"
     gene            complement(4321..5061)
                     /locus_tag="VN968_25520"
     CDS             complement(4321..5061)
                     /locus_tag="VN968_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HXL32623.1"
                     /translation="MKLGAGEDASAGNSIDPRPVLIVTGLVQEARIAAGPGMTVICSS
                     SDPQQLRALLTVFDPSTIRGVISFGVAGGLDPTLKSGDVVVATEVLSGDARWLAGLTL
                     NEDLIARVALGRRRVVRGGLAGVEQVILARAGKAALHSKTGAAAVDMESHIAAAYAAE
                     AGLPFAALRVISDPAHRALPALAMAAIKPNGDIDLRRVLRGLARNPSTLRALVSTGID
                     FNRALKSLRGCRGFLLGGGGEGLVAADL"
     gene            complement(5058..7034)
                     /gene="shc"
                     /locus_tag="VN968_25525"
     CDS             complement(5058..7034)
                     /gene="shc"
                     /locus_tag="VN968_25525"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HXL32624.1"
                     /translation="MHSISKSAGIETGAASDAALEASIASATSALLGDRRPDGHWVFE
                     LEADSTIPAEYVLLRHYLGEAVDTELERKIANYLRRVQGAHGGWPLVHDGDFDMSASV
                     KSYFALKMIGDPADAPHMARAREAIRSRGGAIHSNVFTRFMLAMFGVMTWRSVPVLPV
                     EIMLLPMWSPFHINKISYWARTTIVPLMVLAALKPRAQNVGGVGIDELFLQPPHSIGM
                     TAKAPHQSWGWFLLFRGLDSVLRVIEPLFPKKQRARAIDSAVAFIEERLNGEDGLGAI
                     YPPMANAVMMYEVLGKPADYPPRAVTRRALDRLLVIRENEAYCQPCMSPVWDTSLTCH
                     AMLEAAGGEALPAAKQGLDWLLPKQVLDVKGDWAVKRPDVRPGGWAFQYNNAYYPDLD
                     DTAVVVMAMDRARHASQNRAYDAAIARGREWIEGMQSRDGGWAAFDVNNLEYYLNNIP
                     FSDHGALLDPPTEDVTARCVSMLAQLGANVQGSQAVADGVAYLRRTQLAEGSWYGRWG
                     LNYIYGTWSVLCALNAAGVDHQDPVIRKAADWLLSIQNKDGGWGEDATSYRLDYRGFE
                     GAPTTASQTAWALLGLMAAGEVGHPAVTRGVEYLKSTQTRKGLWDEQRYTATGFPRVF
                     YLRYHGYSKFFPLWALARYRNLKGTNSRVVGVGM"
     gene            complement(7107..>7733)
                     /locus_tag="VN968_25530"
     CDS             complement(7107..>7733)
                     /locus_tag="VN968_25530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL32625.1"
                     /translation="IKLLQDKGASVQFGHELRELVMSANRVGELRFGDDAVTLAPDDV
                     VVMAVPPRPAASLLPGLKTPAKFRAIVNAHFRFDPPKDLPPILGVVGGLVEWLFAFPQ
                     RLSVTISSADRLVDTPREELAQAIWRDVCKAGGVEGELPPWQIVRERRATFEATPEQN
                     ALRPGPVTGWKNLFLAGDWTDTGLPATIEGSVRSGDRAADLVLAMRRA"
BASE COUNT         1523 a         2461 c         2350 g         1399 t
ORIGIN      
        1 gcgccggggc ccttggcatc gccggcggtc ctgcgcaggt tttccgccga tcccttcaag
       61 gactcgacat tctcctgatc ggacggcggg gcatcgatcg agtccgggtt gagcgcggga
      121 ccaagaatct tggcgccttg cccgatcagc ttcagttttg ccggctggtc cgccggcaca
      181 aaactgtcca gcgacatcgc gcggagcact tccggtacct tggacagccg ttcttcgatc
      241 ttcttggcgt cggcgtccga gttcgtcatc acattgatgg cgttggcgcc ggtgttcgga
      301 tcgctgcgca gatcgagaaa agtcccgatc gattccacct tgggatttcg caggttgacc
      361 gggttgaaat cgaacctcag gaaataaagc agcggcagac cggcgaccgc gatcaaaagc
      421 gtgccgacga tgatgacgac gcgatgcttt tcgaggaaat aatccaccgg cgccaggaac
      481 gcatagccga ccggctccga ttccccggga ggattgagca gcttcagcaa agccggcagc
      541 accgtgatgc tggtcgtaaa ggcaaccagc atcccgacgc ctgcgatctt gccgagttcc
      601 gaaatgccct tgtagtcggt tggcaggaac gagaggaatc cggccgccgt cgccatcgcg
      661 gcgagtgaaa gcgggaccgc ggagcgttca gcggcctttt ccagcgccag ccgcaggtcg
      721 tcgcctttga atcgctccga ccggtagcgg acgctgaact ggataccgaa gtcgacgccc
      781 agtccgacga acagcacggc gaatgcgatc gagagcaggt tcagcgaacc caccatccac
      841 agccccacgg cggtggtgat ggaaaggccg ataaagaggt tcaggaacac ggcgaagatg
      901 atctttgtgg aatgcagcgc catccagaga atcaacagca ccacgagcac ggtgccgatc
      961 ccgttgacga tggcgccatc ctggacggtg gcgaattcct cgttggcaat cggaaccggt
     1021 ccggtcagcc tgacccgcgc gccatattcg cctgcgagat tgagatcgcg cgccgcctgc
     1081 cggatggcat cggtcgcatc cttgcccggc tcgagcgcct tgaagtcgag gatcggcttg
     1141 aactcgatga acgagcgccg atcggcgtcg gtcaaaggct tgtcgctggt gagttcgcgc
     1201 caggagaagg tcccggagcc gttcttcatc acgttctcta cggtctgggc gatcagcgtg
     1261 aatggccgct cggtgccgtc gagtttgacc tgcccgcgct tgacgccagc gagcccggtt
     1321 tccagcgcgc cggtcattcc ccgtatgctg ggatcgcccg ccatgatctc gatcagcggg
     1381 gctgcggatt cgaactggcc ggtcaactgg ctcacttcgg ccaccggcag gaacaacagc
     1441 ccgttctttt caaagaacgc tccggagccg agcggctgga ccgattcgaa atgcacgacg
     1501 tcgccggaca atttctgcgc caatgcgtcc gccgcagcgc tggttagctc cggcgtcggc
     1561 gcgtccacca cggccaggat cgttcgatcc cgatcgaacg ccttctcgaa ctgattgtcg
     1621 cgcttgcgcc agtccagatc gggcgagatc agggtgttga tgtcggtgtt gatgataaag
     1681 ttccgggacg tatagtagcc tgccgtaatc gcaaggataa ctgcgaccag gatcgtgaga
     1741 gacgcgaacc gcgtgcaagc tttcacaact gagacaacaa tacttgtcag cacattttct
     1801 ctttctgata ttattgagct tgccgggaac tacggagagc ttgccggaaa cgcacgctgt
     1861 ctaccggagt tccgtcggcc gatctaatgt tgttttgagc tttttcctca agacaccaac
     1921 aagtggcgca aaagatgccg aaactgcgct ggagcggctc acattatcgg gaccggcagc
     1981 tagcgtaacg aacaagtgag ggttttggcc tggtttggca cggtttcatg gtaaactgta
     2041 gtatgatacc ttaaataccc ttatgggcct gccgccccgt gggccgatcg ttgccaatga
     2101 tccggctttc ctagtccatg gttttttgac acaaagccgc gtgaccccat gcgcttaggc
     2161 gcaagggaca cgaaacccgc tcatggttcg gatgttgcat aaatcacaga cccaagccgc
     2221 gcgcagatta ctgattgagt ttggtgcaac tagcgtaacg gaaatccccc aatggaagta
     2281 tatcttgcgc agccccgcgg attttgcgcg ggcgtggtac gtgcaatcga gatcgtcgag
     2341 cgcgcgctcg agaaatatgg tccgccggtc tatgtccggc atgagatcgt ccacaacaaa
     2401 tacgtagtcg aaagcctgaa gaacaagggc gcgatcttcg ttgaggatct gtcggaggtg
     2461 ccgcctctcg cggtcaccgt attcagcgcc cacggcgtgg ccaggagcgt cgaggaagaa
     2521 gccgccgtcc gcggcctgcc ggtcctcaat gccacctgcc ctctggtgac caaggtccat
     2581 aaccagggca agcgctacat gtcgaaaggc cgggcgctga tcctgatcgg tcacgccggt
     2641 caccccgagg tcgagggaac catgggtcag gttcccggac cggtacttct ggtacaaaat
     2701 gtcgacgatg tggcagaatt gacactgccg gcggacgcgc cggtggccta tatcacccag
     2761 accaccctca gcgtggatga cacaaaagac ataattgccg cccttcagca gcggtttacg
     2821 gatattcaag gcccggacat ccgggatatc tgctatgcga cacagaaccg ccaatctgcg
     2881 gtaagagacc tgagcaaact cgtcgacgtt atcctggtgg tgggggccac taacagttcc
     2941 aactcaaata ggctgcgcga aatcggcacc gaagtcgggg tcgcgagtta cttgattgcc
     3001 gacggaagcg agctgaaccc ggaatggctg aagcacgcca aggccgtcgg catcaccgcc
     3061 ggcgcctcgg cgccggaggt acttgtcgat gacgtgatcg aggccctgcg acgaatcgga
     3121 ccggtgacgg tgtcggtact tccgggtcgg gaagaaaata tcgaattccg gctcccggcc
     3181 gaactgaccg tgggttaatc cacttcactt aagtccagaa agaaaaccgt aatggcaata
     3241 ccgttcttca aggaaatgcg gatcggcggc tacctgatga agcagaaatt gcttggccga
     3301 aagcgctatc cgctggtgct gatgctcgag ccgctgtttc gctgcaatct ggcctgcgtc
     3361 ggctgcggca agatcgacta tcccgatgcg atcctcaatc gccgcatgtc ggcacaggaa
     3421 tgctgggatg ccgccgacga gtgcggcgcg ccgatggtgg caatccccgg cggcgagccg
     3481 ctgatccaca aggaaatcgg cgagatcgtg cgcggcctgg tggcacggaa gaaattcgtg
     3541 tcgctgtgca ccaacgcgct gctgctggag aagaagctcg acctgttcga gccgtcgcca
     3601 tatctgtttt tctcggtgca tctggacggc ttgaaagacc accacgacaa ggcggtctcg
     3661 cagaaaggcg tgttcgatcg cgcggtctcg gcgatcaagg ccgccaaggc gaagggcttc
     3721 acggtcaacg tcaatgccac gatcttcgac gggcatgccg cagaagacat cgccaaattc
     3781 ctcgacttca cgacggaact cggggtcggc gtttcaatgt cgccgggcta cgcctatgag
     3841 cgcgcccccg accaggagca cttcctcaac cgcaccaaga ccaagaagct gttccgcgac
     3901 gtgttcgcgc tcggcaaggg caagaagtgg aatttcatgc attccggcct gttcctggat
     3961 tttcttgcag gcaatcagac ttacgaatgc accccgtggg gcatgccggc acgcaacatt
     4021 ttcggctggc agaagccctg ctacctgctc ggcgaaggct acaccaagac cttcaaggaa
     4081 ctgatggaga cgaccgactg ggagacctac ggcaccggca agtacgagaa gtgcgccgac
     4141 tgcatggcgc attgcggcta cgagccgacc gcggcggacg ccgcgctgca gaatccgttg
     4201 aaggcaatgt gggtggcgct gcgcggcgtg cgcacgaccg gtccgatggc gcccgagatc
     4261 agtctcgatc atcagcgtcc ggcgcagtac atcttctcgg cggaagtgca gaagcgcctg
     4321 tcagagatcc gccgcgacga ggccctcgcc gccgccgcca aggagaaagc ctcgacagcc
     4381 gcgtaggctc ttcagcgcgc gattgaaatc gatcccggtc gagaccagcg cgcgcagcgt
     4441 cgatggattg cgcgccaggc cgcgcaggac ccggcgcaga tcgatgtctc cgttcggctt
     4501 gatggcggcc atcgccagcg ccggcaacgc acggtgggcg gggtcgctga tcacgcgcaa
     4561 ggccgcgaac ggaaggcccg cctcggccgc gtaggcggcg gcgatatggc tctccatatc
     4621 gaccgcggcg gcgccggtct tcgaatgcag tgcggccttt ccggcgcggg cgaggatgac
     4681 ctgttcgacg cctgccagac cccccctgac gacgcgcctg cgtcccagcg cgacgcgggc
     4741 gatcaggtct tcattcagcg tcagaccggc cagccagcgg gcgtcaccgg aaagaacttc
     4801 ggtcgccacc acgacgtcgc ccgatttcaa tgtaggatca agtccacccg cgacgccgaa
     4861 gctgatgacg ccccggatcg tcgacgggtc aaacaccgtc aggagcgcgc gcagttgctg
     4921 cggatcacta ctgctgcaga tgacggtcat gccgggccct gccgcaatgc gggcctcctg
     4981 caccagaccg gtcacgatca acaccggccg cggatcaatc gaattgcccg cgctcgcgtc
     5041 ttcccccgcc cccagtttca cattccgacc cctaccaccc tgctgttggt gcccttcaaa
     5101 tttcgatacc gcgccaacgc ccagagcgga aagaacttcg aatagccatg gtaacgcaaa
     5161 taaaacaccc gcggaaagcc cgttgccgtg tagcgctgct cgtcccacag cccttttctg
     5221 gtctgtgtgc tttttaggta ctccaccccc cgcgtgacgg cgggatgccc gacctcgcct
     5281 gccgccatca gcccaagcaa ggcccatgcc gtttgtgagg cggtggtggg ggccccctca
     5341 aagcccctgt aatcgagccg gtagctggtg gcatcctcgc cccagccgcc gtccttgttc
     5401 tggatcgaca gcagccagtc ggcggctttg cggatcacgg ggtcctggtg gtccacccca
     5461 gcggcattga gcgcgcacag cacggaccag gttccgtaaa tgtagttcag accccagcgg
     5521 ccgtaccacg acccttccgc aagctgggtc cggcgcagat acgcgacccc gtccgcaacc
     5581 gcctggctgc cttggacgtt cgcaccgagc tgagccagca tcgagacgca gcgggcggtg
     5641 acgtcctcgg tcggcggatc gagcagcgcg ccgtgatcgg agaatggaat gttattcagg
     5701 taatattcga gattgttgac atcgaaggcg gcccagccgc catcgcggct ctgcatgccc
     5761 tcgatccatt cgcggccacg cgcgatcgcc gcatcgtatg cacggttttg gctggcgtgc
     5821 cgcgcgcggt ccatcgccat caccaccacg gcggtgtcgt cgagatcggg ataataggca
     5881 ttgttgtact ggaacgccca accgccaggg cgaacgtcgg ggcgcttgac ggcccagtcg
     5941 cccttgacat ccagcacctg cttcggcagc agccaatcga ggccctgttt cgccgcaggc
     6001 agcgcctccc cgccggctgc ttcgagcatc gcatgacagg tcagcgaggt gtcccacacc
     6061 ggagacatgc agggctggca ataggcctcg ttttcgcgga tcacgaggag ccggtcgagc
     6121 gcccttcgtg tgacggcgcg gggcggataa tcggcgggct tgcccagcac ctcatacatc
     6181 atcacggcgt tggccattgg cggatagatc gcacccaggc cgtcttcgcc gttcagccgc
     6241 tcctcgatga acgcgaccgc gctgtcgatc gcgcgggcac gctgcttctt cggaaacaag
     6301 ggttcgatca cccgcaacac gctgtcgagt ccgcggaaca acagaaacca gccccagctc
     6361 tggtgcggcg ccttggccgt cattccgatg gaatgcggcg gctgcaggaa cagttcgtcg
     6421 atgccgacgc caccgacatt ctgcgcgcgc ggcttcagcg ccgccaggac catcagcggc
     6481 acgatcgtgg tccgcgccca gtacgaaatc ttgttgatgt gaaacggcga ccacatcggc
     6541 agcagcatga tctcgaccgg caataccggc acgctccgcc aggtcatcac cccgaacatc
     6601 gccagcatga agcgggtaaa aacgttgctg tggatggcgc cgccgcgcga gcggatcgcc
     6661 tcgcgcgccc gcgccatgtg cggggcatcc gcgggatcgc cgatcatctt cagcgcgaaa
     6721 taggacttca cactggcgct catgtcgaaa tcgccgtcat gcaccagcgg ccagccgcca
     6781 tgggcgccct gaacccggcg cagataattg gcgatcttgc gctccagctc cgtatcaacg
     6841 gcttcgccta agtagtggcg aagcaagaca tattcggcgg ggatggtgga atcggcttcc
     6901 agctcgaaca cccagtgtcc atccggccgc cgatcgccaa gcagcgcgct cgtcgctgac
     6961 gcgatgcttg cctcaagcgc cgcatcggag gctgctccgg tctcaattcc tgctgacttg
     7021 ctgatggaat gcatctggct cgacatcccc ttaagatgaa cttgaaatga cttgcccggt
     7081 tatagcgaaa ccgactgtga tctcgatcac gcccgccgca tcgccagcac cagatcggcg
     7141 gcgcggtctc ccgaccgcac cgacccctca atggtcgccg gcaacccggt atcagtccag
     7201 tcgcccgcga gaaacaggtt tttccagccc gtgaccgggc ccggccgcaa ggcgttctgc
     7261 tccggggtcg cctcgaatgt ggcgcggcgc tcgcgcacga tctgccacgg cggcaattcg
     7321 ccctcgacgc cgccggcctt gcagacatcc cgccagatcg cctgtgctag ttcctcacgc
     7381 ggcgtatcga ccagccggtc ggcgctgctg atggtgaccg acagccgctg cgggaacgcg
     7441 aacagccact ccaccagccc accgacgacc cccagaatcg ggggcaggtc cttgggcgga
     7501 tcgaagcgga aatgcgcatt cacgatggcg cggaacttcg ctggcgtttt caggcccggc
     7561 agcagcgatg cagcggggcg gggcggaacc gccattacca cgacgtcatc cggcgcgagg
     7621 gtcaccgcgt cgtcgccaaa cctgagctcg ccaacccggt ttgccgacat aacgagctcg
     7681 cgcagctcat gcccgaactg gacgctggcg cccttgtcct gcagcaattt gat
//
LOCUS       DATNOI010000630         4924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2017796,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000630 DATNOI010000000
VERSION     DATNOI010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017796"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(867..1640)
                     /locus_tag="VN968_25535"
     CDS             complement(867..1640)
                     /locus_tag="VN968_25535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL32626.1"
                     /translation="MADPRRSEFGDFLRSRREKLSPKSVGLPSGRRRRTAGLRREEVA
                     ELAGIGVDWYIRLEQGRTVSPSVTTVDALARALRLGKAEHAHLRALASNADRRAFSRE
                     RVPDVIRRLVEGLNQPAYVTGRRWDVLAWNSAADEIFAFSRLAEEDRNTLISVLTNPH
                     TRGLFGSAWADEAKRMVAQFRATHDLWAGDPAFIDLLERLRQGCPEFAPWWEAHDIGS
                     ITAGRKRLSHPKKGSLRFEYATFQANDDPALKLVIYTPV"
     gene            1752..2831
                     /locus_tag="VN968_25540"
     CDS             1752..2831
                     /locus_tag="VN968_25540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HXL32627.1"
                     /translation="MKAAVLRAFGSPLAIETLPDPVLGTGEIIVDVIATRVLSYANEV
                     FSGERKYLLDLPVVPGPGAIGRVRAIGPDATHLVAGDWVCCDPTVRSRDDALNPDITL
                     QGWSARGEGGLRLQKHFRHGSWAEQMRVPTENVKAIGPIDAADAAQWCALGTLLVPYG
                     GFLAANLQAGETVLVSGATGNFGSAAVAVALAMGAACVIAPGRNEQVLADLARRFGDR
                     LRPVKLSGSEDDDRARMQQAAPGPIDCVLDILPPSVSTTTVRAAIMTVRPYGRVALMG
                     GVGMLGGPGLDLPYPWIMRNCISIHGVWMYPPRATIALVNLIRAGLLGLDNFEATTFD
                     LDHANDAVAHAASNSGPFKMTVIRP"
     gene            complement(2857..3555)
                     /locus_tag="VN968_25545"
     CDS             complement(2857..3555)
                     /locus_tag="VN968_25545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32628.1"
                     /translation="MEQGAEDVPVVYLHEIKRQYTQGETTLTILNGAKLALWAGQSVA
                     LVAPSGAGKSTLLHIAGLLETPDDGEVYIGGAPTSGLSDIDRTQIRRTEIGFVYQSHR
                     LLPEFSALENVMLPQMICGLKRSETVKRATEILAYLGLGDRMKHRPAELSGGEQQRVA
                     IARAVANAPRVLFADEPTGNLDPHTADHVFQALTQLVKATHVAMLIVTHNMELAGRMD
                     RRVSLVDGQVVELE"
     gene            complement(3563..4843)
                     /locus_tag="VN968_25550"
     CDS             complement(3563..4843)
                     /locus_tag="VN968_25550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HXL32629.1"
                     /translation="MDESMSEPTRTPPFAPFEWLLSGRYLRARRKEGFISVIAGFSFL
                     GIMLGVATLIIVMAVMNGFRKELLDKILGLNGHLLVQPLESPLTDWKDVAERISQVGG
                     IRLAAPVVDGQALASSPFNAAGVLVRGIRSEDLNNLTSIAKNIKQGTLEGFDEGQGVA
                     IGRRLADQLSLHAGDSITLVAPKGAVTPMGMTPRIKPYKISAVFEIGMTEYDSIFVFM
                     PLPEAQAYFNRPNDVTAIEVFTTNPDKIDVYRKTVTEAAGRPVFLVDWRQRNSTFFNA
                     LQVERNVMFLILTMIVLVAALNIVSGLIMLVKDKGSDIAILRTMGASQGSIMRVFLIT
                     GASIGVVGTLTGLFVGMLICLNIESIRQFLSWLTSTELFSPELYFLSKLPAEIDFGET
                     SAVVIMALTLSFLATLYPSWRAARLDPVDALRYE"
BASE COUNT          935 a         1546 c         1518 g          925 t
ORIGIN      
        1 ccttggccag ttcgccaagc ttctggatct tgatcgaccc cttcagcagc gaataggccg
       61 aatggacgtg aagatggaca aatccggcat tggacatggt cgccgaaggc ttctgcatca
      121 tggacttaaa gcgatcactg ggcttgaggg agcgctgtca gcctcgaagg ccgactcgcg
      181 cctcaatggt gatgcctcgg gctcgatgtg tccacgcgga acgggcaatt gatgcccgcc
      241 gtcccggttt tccccagccc ctgtcttttt cctcaactcc tgtctgcgag ccgcgccttg
      301 acatacaagc ccggctcccg cgcttacctc agagttgggg aatcagctgc gcccagatgg
      361 cgatcattcc gacgaaaagt gtgatcgacg ccagcgccgc agcctcttcc acgaatatct
      421 tcaacatgcc tacctcctga tacggaacat aagaagaaca ttgttcctat tttgttccga
      481 tctgtcaaga ggccggttat cgatcagttt gaaggatgac ctgatcgtta attggatggc
      541 tcgggcggca tgccgggcgg cggttcgtca tgctcaactc gacggatgag gcctgtgggt
      601 atggggcctt cctcgcgctt tgctcaaatc cgaatcggat caacttcccc gccgcgccgc
      661 attccgctca cggaatggcc cttccgattg cgtatgatcg aggcgcgaat tggatacccg
      721 gaacacttcg cgcaacatcg tgagttgacg ggaacccgcg cgcccggttt ggcatccggc
      781 cgcccggccc aatgcgtgtc caatccgtat gtcgcgagcg ccgatgacgg gccgccggaa
      841 ccacgctacc ggcatcggcg cgtctactag accggcgtat agatcacaag cttcagcgcc
      901 ggatcgtcat tggcctgaaa ggtcgcatac tcgaagcgca gcgagccctt tttgggatgg
      961 ctgagccgtt tgcggccagc cgtgatgctg ccgatgtcat gtgcctccca ccatggcgca
     1021 aactccgggc aaccttgccg aaggcgctcc agcagatcga taaaggcggg atcgccggcc
     1081 cacagatcgt gcgtggcgcg aaactgggcc accatgcgct tggcctcgtc ggcccaggcc
     1141 gatccgaaca ggcctcgcgt atgcgggttg gtcagcacgc taatgagcgt gttgcggtcc
     1201 tcttccgcca ggcggctgaa ggcaaagatc tcgtcagcgg cagagttcca cgccaggaca
     1261 tcccagcggc ggccggtgac gtaagcgggc tggttgaggc cctcgaccag gcgccggatc
     1321 acgtcgggga cgcgttcgcg cgagaaggcg cggcggtcgg cgttcgaagc caaggcccgc
     1381 aaatgagcat gctccgcctt gccgagccgc agcgcgcgag ccagagcatc gacggtggtg
     1441 accgaagggc tgacggtccg tccctgttcc agccggatgt accaatcgac accgatgccg
     1501 gcgagttcgg ccacctcctc acgccggagg cccgccgtgc gccgcctccg cccgcttgga
     1561 agaccaacgc tcttggggct tagcttctca cgtcgcgatc gcagaaaatc gccgaactcg
     1621 cttcgacgcg gatccgccat gaccatcacg ccttcacttg ggagggacca tccaatccta
     1681 ggataatacc aggccttcct gcgatcggaa agtctgcgat catgatcgcc tttccagaaa
     1741 gcaggatcat catgaaagct gcagtcctca gggcgttcgg atcgccactt gccatcgaga
     1801 ccttgcccga cccggtgctg ggtaccggcg agatcattgt cgatgtcatc gcaacccgcg
     1861 tgttgtccta tgccaacgaa gtcttcagcg gcgagcgcaa atacctgctg gacctgccgg
     1921 tggtacccgg acccggggca atcggccggg tccgcgcgat cggaccggac gcgacgcatc
     1981 tcgtcgccgg tgattgggtc tgctgcgacc ccaccgtgcg ctcgcgcgac gatgcgctga
     2041 accccgatat cacactgcag ggctggagcg cccgtggcga gggcggcctg cgcctgcaga
     2101 aacatttccg ccatggctcg tgggccgagc agatgcgggt tccgaccgag aatgtcaaag
     2161 cgatcgggcc gatcgatgcg gccgatgccg cgcaatggtg cgcgctgggc acgctgctcg
     2221 tgccctatgg gggttttctc gcggcgaact tgcaggccgg agaaacggtc ctggtcagcg
     2281 gcgccaccgg caatttcggc agcgccgccg tcgcggtcgc cctcgccatg ggagcggcct
     2341 gcgtcatcgc gccgggccgg aacgaacaag tgctggcgga tctggcgcgc cgatttggcg
     2401 accggcttcg acccgtgaag ctcagcggca gcgaggacga cgatcgggct cgaatgcagc
     2461 aagccgcacc cggcccgatc gactgcgtgc tggacattct gccgccctcg gtcagcacca
     2521 cgaccgtgcg agctgcgatc atgacggtgc gaccctatgg cagggtggcg ctgatgggag
     2581 gcgtcgggat gttgggcggc cccggactgg atttgcctta cccgtggatc atgcgaaact
     2641 gcatcagcat ccacggggtg tggatgtatc ccccgcgggc caccatcgct ctggtcaacc
     2701 tgattcgcgc cgggctcctc ggactggaca atttcgaagc tacgaccttc gacctggacc
     2761 acgccaacga cgccgtcgcg catgccgcgt ccaatagcgg tcccttcaag atgaccgtga
     2821 tccggccttg atcaccttga tgttctggag cctggtttat tccagttcga cgacctggcc
     2881 gtccaccagc gatacgcggc ggtccatccg gccggccagc tccatgttat gcgtgacgat
     2941 cagcatcgcc acgtgggtcg ccttgaccag ttgcgtcagt gcctggaaca catgatcagc
     3001 agtgtgggga tcgagatttc cggtcggctc gtccgcaaac agcacgcgcg gcgcgttggc
     3061 gaccgcacgc gcgatcgcca cccgctgctg ctcgccgccc gacagttcgg ctggccggtg
     3121 cttcatgcga tcgccgaggc cgagataggc gaggatttcg gtggcgcgtt tgacagtctc
     3181 ggaacgcttg aggccgcaga tcatctgcgg cagcatgaca ttttcgagcg cggagaattc
     3241 cggcagcagc cggtgcgact gatacacaaa gccgatctcg gtgcggcgaa tctgggttcg
     3301 gtcgatgtcg gataatccgg aagtcggcgc gccgccgata tagacctcgc cgtcatcggg
     3361 ggtttcgagc aggcccgcga tatgcagcag cgtggactta cctgcacccg acggcgccac
     3421 cagcgcgacc gactgtcccg cccacagcgc gagcttggcg ccgttgagaa tggtcagtgt
     3481 cgtttcccct tgcgtgtact ggcgttttat ctcgtggaga tagacgaccg gtacatcttc
     3541 cgccccctgc tccatctcgc cctcactcgt accggagcgc gtcgacggga tcgaggcggg
     3601 cggcacgcca tgacggatag agcgtggcga ggaaggacaa cgtcagcgcc atgatcacga
     3661 ccgcgctggt ttcgccgaaa tcgatctcgg ccggcagctt ggagagaaaa taaagctccg
     3721 gcgaaaacaa ttcggtgctg gtgagccagg ataaaaactg ccggatcgat tcgatattca
     3781 ggcagatcag catgccgacg aaaagcccgg tcagggtgcc gaccacaccg atcgaagcgc
     3841 ccgtaatcag gaacacccgc atgatcgatc cctgcgaggc gcccatggtg cgcaggatcg
     3901 caatatcgct gcccttgtcc ttcaccagca tgatcaggcc cgagacaata ttcagcgccg
     3961 cgaccaggac gatcatggtg aggatcagaa acatcacgtt gcgctcgacc tgaagcgcgt
     4021 tgaagaaggt cgaattgcgc tgccgccaat ccaccagaaa caccggtcgt ccggcggcct
     4081 ccgtgaccgt cttgcgatag acgtcgatct tgtccggatt ggtggtgaac acctcgatcg
     4141 cggtcacgtc attgggacga ttgaaatagg cctgggcttc cggaagcggc atgaacacaa
     4201 agatcgagtc atattccgtc atgccgatct cgaacacggc cgagatcttg tacggcttga
     4261 tgcgcggcgt catgcccatc ggggtcaccg cacctttcgg tgccaccagc gtgatgctgt
     4321 ctccggcgtg cagcgacaat tgatcggcga gcctgcggcc gatcgcgacg ccctgccctt
     4381 cgtcgaaccc ctcgagcgtg ccctgtttga tgttcttggc gatcgaggtc aggttgttga
     4441 gatcctcgga gcgaatgccg cgaacgagca cgcctgctgc gttgaacggc gaggatgcca
     4501 gcgcctgacc atccaccaca ggtgcagcga ggcgtatgcc gccgacctga ctgatgcgct
     4561 cggcgacatc cttccagtcg gtcagcggcg attccagcgg ctgcaccaga agatggccgt
     4621 tgaggccgag aatcttgtcc agcaattcct tgcggaagcc gttcatgacg gccatcacga
     4681 tgatcagcgt cgcaacgccg agcatgatgc caaggaacga aaagccggcg atgacggaga
     4741 taaaaccttc cttgcgacgt gcccgcagat agcgtccgga caacagccat tcgaatggcg
     4801 cgaaaggtgg ggttcgagtg ggctcgctca tggattcatc catagtccga ttttcacacg
     4861 attcgggcca attgtggccg caacgatcga tctcgctgca tctgcggtgg ataattcgtc
     4921 acgg
//
LOCUS       DATNOI010000631        13706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1336009,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000631 DATNOI010000000
VERSION     DATNOI010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13706
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336009"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VN968_25555"
     CDS             <1..251
                     /locus_tag="VN968_25555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32630.1"
                     /translation="VDELKRETGNRKVKRFRLKRKRLGISIRQIDASDFVGAPGQGGA
                     DLIAHGADIGRRLELPQHCVQSFSKILGDAIEQECCGP"
     gene            482..1276
                     /locus_tag="VN968_25560"
     CDS             482..1276
                     /locus_tag="VN968_25560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32631.1"
                     /translation="MLRPSYGIVRTVITILASTAMSVVIAPVVGEFFIGIAKEHHIYE
                     LPYKWPGAAMSWLSAISGLSWLREVAIFLAGLTIGMWFDAFARRRGAPPKGQPAPVVS
                     NWLDPLVAIERYADPELRTQRDSLREVAERLKTQIDLLKQQWDAEVQKLPFGPAPNHD
                     SDLVAELGQKHRSACDQLHQLGEQVWVMEREITNRIIGQLKNGELAAKGFLHTKDTVS
                     ESSDTIPKDQWQLLKFDTYDTHMQTARGGNWKYIGLQIGRNENKRR"
     gene            1634..2365
                     /locus_tag="VN968_25565"
     CDS             1634..2365
                     /locus_tag="VN968_25565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32632.1"
                     /translation="MTNDDRHNNIAGVLLILGLGLLGWLWCVLLFATSGEQAVTLGAI
                     TGGMIGAGGAVYAVYMTLTRQRREDIAKVCSAVRIEVTTYCKHVIGALNICEQIATKG
                     LSVPMQDAVYVAKSLVEPIIYPAVADRIGLLPHPQATIEFYMRIAEAKAMLLAMQMKT
                     NSPSAINVSAIPLIAPANAEAVAECFITALQLAKHIVAADDPSRTELDLWVQKITLGK
                     IDAALASAKVVFPNATSFQDDQPAA"
     gene            complement(2470..2988)
                     /locus_tag="VN968_25570"
     CDS             complement(2470..2988)
                     /locus_tag="VN968_25570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32633.1"
                     /translation="MPAIKIARMLPRIPPDPEPTEKQIDAELRKVKFAKSLPSEMQRK
                     FAAVFWRADQMRKFPDRFWHPFAPDPMWYNAYLNSDTWRRMSKKIKADAGHKCSCCPK
                     KSTQVHHRCYRPRVLSGEDTSLLIALCGRCHKAVDRDERGEPREANSKERTLAEMFDR
                     ESKRLALSDRST"
     gene            3216..4457
                     /locus_tag="VN968_25575"
     CDS             3216..4457
                     /locus_tag="VN968_25575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32634.1"
                     /translation="MFGFGKKTIPPGEFGLWVLKYADEFVSTDAFRSLASCFPDGYDA
                     SRGWVPVFESNGIPIPTVKLYLRQYTHCVLQSVFREHSQSQRRAMVHGAISGLQETPT
                     GYDFEKTFGNLETVFEGNYEFDPRVGSLNDDGRLNYLTVPNAHILVSKYLIDRFVLHN
                     MKNSKALIDNFGLYSSTVACSLAAAHRAILSITSKVKISPEPASSPKIRPQVSPSRPQ
                     AQAPKADPPPATPRWTPVAQGQPKAEPPAPPPKSKPYSGKVSVDWSAWMIVGNDLVRQ
                     FDFQNLPFAEKLMLQIRVTVKMGSFGVAYSHPHLIIQPPISREREIKRTILCDPEDGT
                     TEVGPMEVTIAHVPGDASMFWFFEENSRKFLTVLYAMKRLRFILLGPDGEEIQFALPL
                     HNDPTYRTEFRRIKDQVTAAH"
     gene            complement(4507..4662)
                     /locus_tag="VN968_25580"
     CDS             complement(4507..4662)
                     /locus_tag="VN968_25580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32635.1"
                     /translation="MPVNKPPDGQITGLRREHIPRVADAVQYHQRVHTRLDVPRLLCG
                     ALLIRGP"
     gene            5347..6156
                     /locus_tag="VN968_25585"
     CDS             5347..6156
                     /locus_tag="VN968_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HXL32636.1"
                     /translation="MDAEASPSRSVATHLRGALNVCRGAWAHAARLALDIALPTLCVA
                     RREPVAGEGVCAACWAKLSFIAQPYCPRLGIPFVYDPGPGLLSMEAIADPPAYARARA
                     AVRYDDVARTLVHALKYQDRTDLAPAMGRWMARAGRELLGEADALVPVPLHWRRGWSP
                     RYNQSGALARVIERQTGVKVTSEALRRVRPTQQQIGLSRSQRASNVQGAFKVEASRQS
                     EIQGRRVILIDDVLTSGATVDACARALLRAKAASVDVLVFARVVDTHKAPI"
     gene            6186..6461
                     /gene="grxC"
                     /locus_tag="VN968_25590"
     CDS             6186..6461
                     /gene="grxC"
                     /locus_tag="VN968_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HXL32637.1"
                     /translation="MTAAIEIYTRPGCGYCSAAKSLLTRKKAAFTEHDVAIDSTLRQR
                     MWDRAGAGSTFPQIFIGETHVGGCDELYALDRAGKLDSMLAGEKANS"
     gene            6458..7336
                     /locus_tag="VN968_25595"
     CDS             6458..7336
                     /locus_tag="VN968_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HXL32638.1"
                     /translation="MSDDLSFTAAMVQMRTGLLPEPSLEQGTKLIRQAAAEGADYVLT
                     PEVSNMMQLNRTALFEHLAAEEDDLSLKAYRALAAELKIHLHVGSLALRASPERAVNR
                     SFLIGPDGLVIASYDKIHMFDIDLPDGESYRESANYQPGETAVISDLPWGRIGLTICY
                     DVRFPALYRALAESGAAFLTVPSAFTRKTGEAHWHTLLRARAIENGCFVFAAAQGGMH
                     ENKRETYGHSLIVAPWGEVLAEGGVEPGVFMAKIDPAKVETARKTVPSLQHGRRFSVA
                     DPKAGPEHLHLVRGSA"
     gene            7333..7809
                     /locus_tag="VN968_25600"
     CDS             7333..7809
                     /locus_tag="VN968_25600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HXL32639.1"
                     /translation="MILYALRCERGHAFESWFQSSSAYETQEKRKLVNCPVCGSAKVE
                     RAIMAPQIVSKKGRDSAVPAPAAPTPTDITTPTSTPLLMAQERELRAKLRELRDHIVK
                     NADNVGERFPNEARKMHYGDIEHRPIYGEASPDEARALIEEGVEVSPLPVLPDDRN"
     gene            complement(7810..8709)
                     /locus_tag="VN968_25605"
     CDS             complement(7810..8709)
                     /locus_tag="VN968_25605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXL32640.1"
                     /translation="MLGIATLWIPFTIVAALGQVARNAMQRQLTAPLGTWGATNIRFL
                     FGFPFSLIFFAVVIAATGDAVPWPTSAFWPWLLLGALSQIVGTGMMLLAMNDRSFVVT
                     TAYLKTEAIQTAIFGFVFLGDHLTLSKVIAILIATAGVVIAALRPGAEKGFADLKPTL
                     LGLAAAAAFALSAVGFRGAVIVVPGVSFVTAASYTLVFGLFVQTLALSIYLLVRAPDV
                     LTKILRLWKPSLFAGFMGAFASQFWFLAFALTAAANVRTLALVEVLFAQAVAYYSFKQ
                     PLSGRELGGIALIVIGVALLVSV"
     gene            complement(8871..9641)
                     /gene="ubiG"
                     /locus_tag="VN968_25610"
     CDS             complement(8871..9641)
                     /gene="ubiG"
                     /locus_tag="VN968_25610"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HXL32641.1"
                     /translation="MAMQQNSPGGLSGAPASTVDPAEIAKFSKLSDEWWDPNGKMAPL
                     HKINPLRLTYIRDAACRKFERNVKSLNCLSGLRILDIGCGAGLLCEPFTRLGAQVIGV
                     DPSATNIAAAKLHADKAQLSIDYRCTTVEQMDVRERFDIVLAMEVIEHVTDVGVFLNR
                     CAAMLKPGGLMVISTLNRNWKSFALAIVGAEYVLRWLPRGTHQWDRFVTPDELAHHLE
                     NNKLAITEQAGVVYSPFADRWSISSDMDVNYMVVAEGV"
     gene            complement(9658..10125)
                     /locus_tag="VN968_25615"
     CDS             complement(9658..10125)
                     /locus_tag="VN968_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXL32642.1"
                     /translation="MGRYIDDILQPGEKVLYSTNAHWIFYWPAIAAWILAVVLFFLSR
                     ATVTEGIVLLYLSASAVVAVAALYWTFKAWFHRWTTETDVTNLRVVHKSGFIKRRTFE
                     MSLDKVESVDVDQSILGRILNYGNVTVRGVGEGAETITTIASPLDFRNHITAR"
     gene            10297..11550
                     /locus_tag="VN968_25620"
     CDS             10297..11550
                     /locus_tag="VN968_25620"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HXL32643.1"
                     /translation="MGRLVMKFGGTSVANIDRIRNVARHVKREVDAGHDVAVVVSAMA
                     GKTNELVEWCRDASPMHDAREYDAVVASGEQVTAGLLAVALQAIGIQARSWQGWQIPI
                     RTSDAHASARILEIDGSELINRFKDRKEVAVIAGFQGINPQTGRITTLGRGGSDTSAV
                     AIAAAIHADRCDIYTDVDGVYTTDPRVVPKARRLDKIAFEDMLELASQGAKVLQVRSV
                     ELGMVHNMPVFVRSSFDKPDDIDPHATPPGTLICSEEEIMESHVVTGIAFSKDEAQIS
                     VRQIQDKPGVAASIFGPLAAANINVDMIVQNVSEDGATTDLTFTVPASDYTRARDTIT
                     SAKSRIGYARLDSATDVAKVSVIGSGMRSHAGVAAQAFAALAARNINIRAITTSEIKF
                     SVLIDAAYTELAVRTLHTLYGLDHT"
     gene            11817..>13706
                     /locus_tag="VN968_25625"
     CDS             11817..>13706
                     /locus_tag="VN968_25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HXL32644.1"
                     /translation="MRSASGGPRVLLRRLRETMAEQVSAQERLDKIVVLIAANMVAEV
                     CSTYVLRVDNTLELYATEGLNRDAVHRTVLSAHEGLVGLVASEATPLNLSDAQNHPAF
                     SFRPETGEEIYHSFLGVPILRAGNTLGVLVVQNRAKRTYVEEEVEALQTTAMVLAEMI
                     ASGELSALAQPGAEPAARHSVHKTGAILSDGIALGHVVLHEPRVIITNYIADDLPKEI
                     KRLDAALTKLRADLDRMLERGDVAEGGEHRDVLEAYRMFANDHGWSHRLHEAVATGLT
                     AEAAVERVQSDTRARMLRSTDPYLRDRLHDLEDLGHRLMRQLVGQDHAPSREQLPDNA
                     ILIARAMGPAALLDYDRKRLRGLVLEEGTANSHVSIVARALGIPAVGEVPNAPGIADP
                     GDAIIVDGTSGSIYVRPSAEIESAYAERVRFRARRQAQYSALRDKPCVTKDGQHIELL
                     INAGLIIDLPHIDDSGSAGIGLFRTELQFMVGQSLPRTSDQLALYRAVLDAAGPKPVT
                     FRTLDIGGDKALPYMETVIEENPALGWRAIRLGLDRPGLLRGQVRALLRAGGGRALRI
                     MFPMISEVAEFDAGKSIVERELTYLRQHGHALPERIDVGTMVEVPALLYQLDELLKKV
                     DFISVG"
BASE COUNT         2773 a         4225 c         4176 g         2532 t
ORIGIN      
        1 tcgttgacga actgaaacgc gagaccggaa accgcaaggt caagcgattc cggctcaagc
       61 ggaagcggct cggaatttcg atccggcaga tcgatgcgag cgattttgtc ggcgcgcccg
      121 gtcaaggcgg tgcggacctg atcgcccacg gtgccgatat cggccgccgt cttgaattgc
      181 cgcagcactg cgtgcaatcg ttcagcaaga tcctcggcga cgcgatcgag caggaatgtt
      241 gcggaccctg atcgcaatgc gcgcgactgc cgcgcccgca acaaggcgcg gtcgaacagc
      301 atgggcgcgg tggtcgggtt cggagccatg ccgattggta cgccgacagg cgggtccaac
      361 gcaatcatgc ggtattatgg cgcaaacgcc accgggggcc gaaagtgacc cggatccgcc
      421 ccagtccact gcattcccag gttgccgttc ctagggtact ttgcccaagg ggagaacttt
      481 tatgcttaga ccgtcctacg gtattgtgcg caccgtcatc actatcctcg catcaaccgc
      541 tatgtcagtt gtcatcgcgc cggttgtcgg ggagtttttt atcggcatcg caaaagagca
      601 tcatatttac gaattgccct acaagtggcc cggggcggct atgagttggc tcagcgcaat
      661 atccggactg tcttggctac gcgaggtagc tatctttctg gcgggcctca ctatcggaat
      721 gtggtttgac gcatttgcgc gacgacgcgg cgcaccccca aagggccaac ctgcgcctgt
      781 agttagtaat tggcttgatc cgctggtagc cattgaaagg tacgccgatc cagagttgcg
      841 cactcagcgc gattctctac gggaagtcgc ggaacgcctc aaaacacaga ttgatttact
      901 aaagcagcaa tgggacgcag aagtgcaaaa gcttccgttc ggtcccgccc ccaaccatga
      961 ttcagattta gtcgcagagt tgggtcaaaa acatcgctcc gcttgtgatc aattgcatca
     1021 attaggcgaa caagtttggg tgatggagcg tgaaataact aaccgcataa ttgggcaact
     1081 taaaaacggc gagttagccg caaagggatt tctacatacg aaagataccg tttccgaatc
     1141 ttctgataca attcccaaag atcaatggca gttgcttaag tttgatactt acgacacgca
     1201 tatgcaaact gcgaggggtg gcaactggaa atacatcggc ctgcaaatag gtcgcaacga
     1261 aaacaaacgc cgttagattt catctaggac cactccctcc aattccaagg ccgctggatt
     1321 ccatcgatgc tggcgtgcct cttgccgaag gcaagaccgc gcctgatgcc tccgatcttc
     1381 tagagactga gcggcttcag aaaatcaggc tagaaacaag ttttcccggt cccgccttaa
     1441 ggacgtcaaa gctgaccgtc cgcttcgcgg gcgcgcttta tgcattgatt atccggagcg
     1501 cgccgcgatc tgtgcaacga agcatgatga ggttttgcat cgacatcgac agcttgcgcg
     1561 gcgcgccgcg cgtcgtgcat tgacccgttc aaccgggcag cgtacatgca gcacggggat
     1621 ggggcgcgcg ccaatgacga acgatgatcg ccataacaac atcgctggcg tgttgctgat
     1681 tttgggcctc ggccttttgg gttggctatg gtgcgttctt ctctttgcca cgtcgggcga
     1741 gcaggccgtt acgctcggcg caatcaccgg tggaatgatt ggggcgggcg gggccgtcta
     1801 cgcggtgtat atgacgctaa cccgccaacg aagggaagat atcgcgaaag tctgctccgc
     1861 ggtgcggatt gaagtgacga cctattgtaa gcatgtcatt ggcgcgctca atatctgtga
     1921 gcaaatcgcg accaaaggct tatccgttcc gatgcaagat gcagtctatg tggcgaaaag
     1981 tcttgttgaa cccatcatct atcctgcggt cgcggacaga attgggcttt tgccgcatcc
     2041 acaagcgact atcgaattct acatgcggat tgccgaagcc aaggcgatgt tgctcgcaat
     2101 gcagatgaag acaaattcgc catccgcgat caacgttagt gccattcctc tgatcgcgcc
     2161 cgcgaacgcg gaggccgtag cggaatgctt cataacggcg ttgcagcttg cgaagcatat
     2221 cgtcgccgca gatgacccta gtcgaacgga gttggaccta tgggttcaaa aaatcacgct
     2281 tggcaaaatc gacgcagcgt tggcgtcggc aaaagttgtt tttccaaacg cgacatcatt
     2341 tcaagacgat cagccagcgg catagcaaaa aacgaagccg ccgccggcaa cggctttcca
     2401 gaaacgcggg tgtgatacgc aacccgtgca ccaatgtcgc tgcatcagat gaagaaaagg
     2461 tttaggccgt catgtgcttc ggtctgagag ggctaatcgc ttgctctcac ggtcgaacat
     2521 ctcggcaagc gtgcgctctt tagagttcgc ctcgcgtggc tcgccgcgct catcgcgatc
     2581 aacagccttg tggcaccgac cgcatagcgc aatcagcagc gacgtatcct cgccagacaa
     2641 cacgcgcggt ctgtagcaac ggtgatgaac ttgcgttgat ttcttcgggc agcacgagca
     2701 tttgtgaccc gcgtccgctt taatcttctt gctcatccgt cgccacgtat ccgagttgag
     2761 ataagcgttg taccacatgg gatcaggtgc gaacggatgc cagaacctat ctggaaattt
     2821 tcgcatttga tccgctctcc aaaagaccgc cgcaaatttt ctctgcatct cgctcgggag
     2881 cgatttagca aacttgacct ttcgcaattc agcatcgatt tgcttttccg ttggctccgg
     2941 atcaggcgga attcgcggca gcatgcgtgc gattttgatg gcaggcacag ggtcaatcct
     3001 cgggcggctg cggtcgccgc cggacagtag ggcagggtgt tgtttcgggg ccatcagaca
     3061 tcagagcgcg ccgcgctgct tcacgcaatc gccctagatg tcgggccgca acgaaccttg
     3121 cccctacagc ctatcgtggg ctgaccgggt acgttgtcgg cgcgaccggg atgtgagaag
     3181 ctactttaag gagaacggcg ggggtagacg cggcaatgtt cggctttgga aagaagacaa
     3241 ttccgcccgg cgaatttggc ctatgggtct taaaatatgc cgacgaattt gtctcaaccg
     3301 atgcctttcg ctcgctcgca tcgtgcttcc ctgatggcta tgacgcctca cgaggatggg
     3361 tgcccgtgtt cgaaagcaac ggcataccca ttccgacagt gaagttgtat cttcggcaat
     3421 atacccattg cgttctccag tcggtattca gagaacattc tcaatctcag cgtcgagcga
     3481 tggttcacgg cgcgatctcg ggattgcaag agacccctac cggatacgac ttcgaaaaaa
     3541 ctttcggcaa cctagaaact gtcttcgaag gcaactacga attcgatcca cgcgttgggt
     3601 cgcttaacga tgatggaaga ctgaattatc ttaccgttcc gaacgcccac attttggttt
     3661 ccaaatattt gattgatagg ttcgttcttc acaacatgaa gaacagcaaa gcgctcatcg
     3721 acaatttcgg gctctacagc agcacagtgg cgtgttcact tgctgccgcg catagggcga
     3781 tcctctccat aacttcaaag gtcaagatca gtcccgagcc tgcatcgtcg ccaaaaattc
     3841 ggcctcaagt gtcgccatcg cggccacagg cgcaagctcc caaggctgac ccaccgccgg
     3901 caactcctag atggaccccg gtggcgcagg ggcaaccaaa ggcggagcca cctgcaccgc
     3961 cgccgaagag caagccgtac agtggcaagg tgagtgtcga ttggagtgcg tggatgatcg
     4021 taggaaacga tctcgtgcgc caattcgatt tccagaactt accgttcgcc gaaaagctga
     4081 tgttgcaaat tcgggttacg gtcaagatgg gaagtttcgg cgtcgcgtac agccatccgc
     4141 acttgatcat tcaaccgccg atctcgcggg agcgcgagat aaaaaggacc atcctatgcg
     4201 acccggaaga cggaacgaca gaagtcggtc ccatggaggt gactattgcg cacgtccccg
     4261 gcgacgccag catgttttgg ttctttgaag agaattccag aaaattcctg acagtgctat
     4321 atgcgatgaa gcggctgcgc ttcatcctgt taggaccgga cggcgaggaa atccaatttg
     4381 cgttgccgct gcacaacgat ccgacgtacc gcacggaatt tcgccggata aaggatcaag
     4441 tgacagcagc gcactgaccc ccagctaccg gggtctgcag agcccggacc catatggacg
     4501 gaagtttcat ggaccccgga tcagcagcgc accacacagc aggcgcggca cgtcgagacg
     4561 cgtgtgaacg cgctgatggt actgcaccgc atccgcgaca cgaggaatgt gttctcgccg
     4621 cagtccggtg atttgcccgt cgggcggctt gttgacaggc atgtcgagcc ccctatgcaa
     4681 aaatatttcg cttcacccgt cgggcaaatc atctctacaa attcgcgcca tcccacccca
     4741 caagaggggc gtatcatgat cgtcacggac gcggggtggg atgcggtgga cgcggcagcg
     4801 ttttgcgcgc gatgggatcg cagggcggat tctcaggaat ccgtgagcgg tcaacagcac
     4861 gcggacgaga gatgttgcag catacggcga agtcgtgtgg tcctgacgcc ccgacgctgg
     4921 cgtcaagttc gcggaggttt tatcggccct accgggcttg gacaaaacct taatccgcga
     4981 atgacggtgg caaaagagcc cggtcaccgg ggagagcacg aaataagtcg taaaaccatt
     5041 gcgtgcggga acgtcgggtg gttccggtgt actcgttgtt actcgtgtgc gttctaccaa
     5101 tacaaactgc acacgaggcc gcgggtgcaa cgggcacccg gcgttcccca cgccctcttt
     5161 gggcgcgaag gtaatagcaa cgcctcgggc gctccgcgtc gcgagaacga agtggtatgc
     5221 gggactctat caacgtcatt gcgagccaac ggggcgcgca ttcgcgcaac ccgttggctc
     5281 gcaatgacga ggaaattcgg ttcgtccgac ttgagcgcca cgtggcgcgg cgctaccctt
     5341 ccccacatgg acgccgaagc ctcaccatcc cgttccgtcg ccacccatct gcgcggcgcg
     5401 ctgaacgtgt gtcgcggcgc atgggctcat gcggcgcggc tggcgctgga catcgcgctg
     5461 ccgacgctgt gcgtggcccg ccgcgagccg gttgccggcg aaggcgtttg tgcagcctgc
     5521 tgggccaagc tgtcgttcat cgcgcagcct tattgcccgc ggctcggcat tccctttgtt
     5581 tacgatcccg gtcccggcct gttgtcgatg gaagcgatcg ccgatccgcc ggcctatgcg
     5641 cgcgcccgcg ccgcggtgcg ctatgacgac gtggcccgca cgctggtgca tgcgctgaaa
     5701 taccaggacc gcaccgatct ggcgccggcg atgggccgct ggatggcgcg ggccggccgt
     5761 gaactgctcg gcgaggccga cgcactggtg ccggtgccgc tgcactggcg gcgcggctgg
     5821 agcccccgct acaaccagtc cggcgcgctg gcgcgggtca ttgagcggca aaccggcgtg
     5881 aaggtgacat cggaagcgct gcggcgggtg cggccgaccc agcagcagat cggcctgtcg
     5941 aggtcgcagc gcgccagcaa cgtacagggc gcgttcaagg tcgaagccag ccggcagtcc
     6001 gagattcagg gccgtcgcgt catcctgatc gacgatgtcc tgacctcggg cgcgaccgtc
     6061 gatgcctgcg cgcgggcgct gctgcgcgcc aaggcggcct ccgtcgatgt gctggtgttc
     6121 gcccgggttg tcgacaccca taaagccccc atataatccg gtaatattct aaagcagagc
     6181 gcaacatgac cgctgccatc gaaatctaca cccgcccggg ctgcggctat tgcagcgccg
     6241 ccaaatcgct gctgacgcgc aaaaaggccg cgttcaccga acacgatgtt gccatcgatt
     6301 cgacgcttcg tcagcggatg tgggatcgcg ccggcgcggg ctcgactttt ccgcagattt
     6361 tcatcggcga gacccatgtt ggcggttgtg acgagcttta tgccctcgac cgcgcgggca
     6421 agctcgattc gatgctggcg ggcgaaaagg ccaattcatg agcgatgacc tcagctttac
     6481 cgccgcgatg gtgcagatgc gcaccgggct gttgcccgag ccgagccttg aacagggcac
     6541 caagctcatt cggcaggccg ccgcagaggg cgcggactat gtgctcacgc ctgaggtgag
     6601 caacatgatg cagctgaacc gcaccgcgct gttcgagcat cttgccgccg aagaagacga
     6661 tctttcgctg aaagcctatc gcgcgcttgc ggcggaactg aaaatccatc tccatgtcgg
     6721 ctcgctggcg ctgcgtgctt cgcccgagag ggcggtcaac cgctcgttcc tgatcgggcc
     6781 ggatggcctc gtgatcgcca gctacgacaa gatccacatg ttcgacatcg atctgcctga
     6841 tggcgagagc tatcgcgagt ccgccaatta ccagcccggc gaaaccgccg tgatatccga
     6901 cctgccctgg ggccggatcg ggctcacgat ctgctacgac gtgcgctttc cggcgctgta
     6961 tcgcgcgctc gccgaaagcg gcgcggcgtt cctcacggtg ccgtccgcct tcacccgcaa
     7021 gaccggcgag gcgcattggc acacgctgct gcgcgcccgc gccatcgaga atggctgctt
     7081 cgtgttcgct gcggcgcaag gtggcatgca cgagaacaag cgcgagacgt atggccactc
     7141 gctgatcgtc gctccctggg gcgaggtgct cgccgaaggc ggcgtcgagc ccggcgtttt
     7201 catggcgaag atcgatccgg ccaaggtcga gaccgcgcgc aaaaccgtgc cgtcgctgca
     7261 gcacggccgg cgcttcagcg tcgccgatcc caaagccggt cccgagcatc tgcatctggt
     7321 gcggggctcg gcatgatcct atacgccctg cgttgcgagc gcggccatgc gttcgagagc
     7381 tggtttcaga gctcttcggc ctacgaaacc caggaaaagc gcaagctggt gaactgtccc
     7441 gtctgcggtt ccgccaaggt cgagcgggca atcatggccc cgcagatcgt gagcaagaag
     7501 gggcgggata gcgcggtacc ggcgcctgcc gcgcccacgc ccacggatat caccacacca
     7561 acgtcgacgc ccctgctgat ggcgcaggaa cgcgagctgc gcgccaagct ccgggaactg
     7621 cgcgatcata tcgtgaagaa tgcggacaat gtcggcgagc gctttcccaa cgaagcgcga
     7681 aaaatgcact acggcgatat cgagcaccgt ccgatctacg gcgaagcgtc cccggacgaa
     7741 gcgcgcgccc tgatcgaaga aggcgtcgag gtctcgccgc tgccggtgct gccggacgac
     7801 aggaattaat tacaccgaaa ccagcagcgc gacgccgatc acgatcagcg cgatgccacc
     7861 gagttcgcga cccgacagtg gctgcttgaa cgaataatac gccaccgctt gcgcgaacag
     7921 cacctccacc agcgccagcg tgcgcacatt ggcggccgcc gtgagcgcaa acgccagaaa
     7981 ccagaactgc gaggcgaacg cccccatgaa gccggcgaac aacgagggct tccacaagcg
     8041 caggattttc gtgagcacgt cgggcgcgcg caccaggaga tagatcgaca gcgccagcgt
     8101 ctgcacgaac agtccgaaca ccagggtata ggacgccgcc gtcacgaacg acaccccggg
     8161 caccacgatc accgcgccgc gaaagccgac cgccgacagc gcgaatgccg cggcggcggc
     8221 gaggcccagc agcgtcggct tcagatccgc aaatcccttt tccgcacccg gccgcagcgc
     8281 agcaatgacg acgccggcgg tcgcgatcag gatcgcgatc accttcgaca gggtcaggtg
     8341 atcgccgaga aagacaaaac cgaagatcgc ggtctggatc gcctcggtct tcagataggc
     8401 cgtcgtcacc acgaacgaac ggtcgttcat ggcgagcagc atcataccgg tgccgacgat
     8461 ctggctgagc gcgccgagca gcagccacgg ccagaacgcc gacgtcggcc acggcacggc
     8521 gtcgccggtc gccgcgatca cgacggcaaa gaagatcagc gagaacggaa agccgaacag
     8581 gaagcggata ttggtcgcgc cccaggtgcc gagcggcgcc gtcaactgcc gctgcatcgc
     8641 gttgcgcgcg acctggccca gcgccgcgac gatggtgaag ggaatccaga gcgtggcgat
     8701 gccgagcatg tgggatcgag ggttagcggg gctgaaattg cggccaccgt gccgatgcgg
     8761 ctgtgcgcgg tcaaccgtca gaattgcatg cggcaccaac cacagcatcg tccctgcgaa
     8821 cgtgttcgca gggacgacaa aaacttggcc taccgacctc aaattccagt tcaaaccccc
     8881 tccgccacca ccatgtagtt cacgtccatg tcggaggaga tgctccagcg gtcggcgaac
     8941 gggctgtaga ccacgccggc ctgctcggtg atggcgagct tgttgttctc aagatgatgc
     9001 gcgagttcat cgggggtgac gaacctgtcc cattgatggg tgccgcgcgg cagccagcgc
     9061 aagacatatt ccgcgccgac gatggccagc gcaaagctct tccagttgcg attcaacgtc
     9121 gagatcacca tcagcccgcc gggcttgagc atcgccgcgc agcgattgag gaacaccccg
     9181 acgtcggtga cgtgctcgat cacttccatc gccagcacga tatcgaagcg ctcgcgcacg
     9241 tccatctgct cgacggtggt gcagcgataa tcgatcgaca gctgcgcctt gtcggcgtgc
     9301 agcttcgccg ccgcgatatt ggtggcggac ggatcgaccc cgatcacctg cgcgccgaga
     9361 cgggtgaacg gctcgcacaa caggccggcg ccgcagccga tgtcgaggat acgcaggccg
     9421 gaaaggcagt tcaggctttt gacgttgcgc tcgaacttgc ggcaggcggc gtcgcggata
     9481 taggtcagcc gcagtggatt gatcttgtgc agcggcgcca ttttgccgtt gggatcccac
     9541 cattcgtccg acagtttcga gaacttcgcg atttcggccg gatcgaccgt ggacgccggg
     9601 gcgccggaca aacctccagg agaattttgt tgcatggcca tgggttgcgc gccgctccta
     9661 tcgcgcggtg atgtgattgc ggaagtcgag cggcgaagcg atcgtcgtga tcgtctcggc
     9721 cccctcgccg acgcctcgca ccgtgacgtt gccgtagttc aagatgcggc cgagaatgct
     9781 ctggtcgaca tcgacgctct cgaccttgtc gaggctcatt tcgaacgtgc gccgcttgat
     9841 gaacccggac ttgtggacca cgcgcaaatt ggtgacgtcg gtctcggtgg tccagcgatg
     9901 gaaccaggcc ttgaaggtcc agtacagcgc cgcgaccgcc accacggccg aagccgacag
     9961 gtaaagcagg acgatgcctt cggtgacggt tgcgcgcgac agaaaaaaga gcacgacggc
    10021 caggatccag gccgcgattg ccggccaata aaagatccag tgcgcattgg tcgaatacag
    10081 caccttttca ccgggctgca ggatatcatc gatataacgc cccatacact tgcctcaatt
    10141 accccaacac cacacggtac gcggctttcg gggatcgccg gaacccggtt gcttgcccct
    10201 gaccgcgccg ctatgtatac gcgccttttg gccggcgcgc ttcggtttcc gtgcgggtca
    10261 cgttacttat cctcttgagg gaatgcacca gtcgtcatgg gccgcctcgt gatgaaattc
    10321 ggcggcacat ctgtcgccaa tatcgaccgc atccgcaatg tggcgcggca tgtgaagcgc
    10381 gaggtcgatg ccggccacga cgttgcggtg gtggtgtcgg cgatggccgg caagaccaac
    10441 gaactggtcg aatggtgccg cgacgcctcg ccgatgcatg acgcgcgtga atatgacgcc
    10501 gttgtggcgt ccggcgaaca ggtcaccgca gggctgctgg cggttgcgct gcaggcgatc
    10561 ggcattcagg cgcgctcctg gcaaggctgg cagattccga tcagaaccag cgacgcccac
    10621 gcgtcggcgc ggattctcga gatcgacggt tccgaactga tcaatcgctt caaggaccgc
    10681 aaggaggtcg ccgtcatcgc cggcttccag ggcatcaacc cgcagaccgg acggataacc
    10741 acgctcgggc gcggcggttc cgacacctcg gcggtggcga tcgccgccgc catccatgcc
    10801 gaccgctgcg atatctacac cgacgtcgac ggcgtctata ccaccgatcc gcgggtggtt
    10861 ccgaaggcgc gccggctcga caagatcgcg ttcgaggaca tgctggaact ggcgtcgcag
    10921 ggcgccaagg tgttgcaggt ccgttcggtg gaactgggca tggtgcacaa catgccagtg
    10981 ttcgttcgat ccagtttcga caagcctgac gatatcgacc cgcacgcgac gccgccgggc
    11041 acgctgatct gcagcgagga ggaaatcatg gaaagccacg tcgtcaccgg catcgccttc
    11101 tccaaggacg aagcccagat ttcggtccgc cagatccagg acaagccggg cgtcgccgcc
    11161 tcgattttcg gcccgctcgc cgcggccaac atcaatgtcg acatgatcgt gcagaacgtc
    11221 tcggaggacg gcgccaccac cgacctcacg ttcaccgttc cggcctccga ttatacccgc
    11281 gcccgtgaca ccatcacctc ggccaagagc cgcatcggct atgcccgtct cgacagcgcc
    11341 accgatgtcg ccaaagtctc ggtgatcggc agcggcatgc gcagccatgc cggcgtcgcc
    11401 gcgcaggcgt tcgcggccct tgccgcgcgc aatatcaata tccgggccat caccacgtcc
    11461 gagatcaagt tttcggtgct gatcgacgcc gcctataccg agcttgcggt ccgcaccctg
    11521 catacgctct atgggctaga tcacacctag aataattttt ccttagcgtg cgcagcatgg
    11581 actgacaaag acgttatggc ctctggcagg cgttttgctt ggcaaaactg gcctcgattc
    11641 gctatacggc ctgatgagat ggctgccgca aactgtgcga gagttttagc cacgctgatt
    11701 cggccggttt ggaagtttgc ggcggcgccg aatgactaaa attgttgctt ttcttgaact
    11761 taccgctagc tgccggcaac cccgggtgct ggcttcgcgg gggaggatac cagcacatgc
    11821 ggagcgcgtc gggaggcccc cgcgtcttgc tgagacggct ccgcgaaacc atggcggagc
    11881 aggtctcggc ccaggagcga ttggacaaga tcgtggtgct gatcgcggcc aacatggtgg
    11941 ccgaggtctg ctcgacctat gtgctgcgtg tcgataacac cctggaactc tatgccaccg
    12001 aaggcctcaa ccgggacgcg gttcaccgca ccgttctgag cgcgcatgag ggtctggtcg
    12061 gcctggtggc aagcgaggcg accccgctca atctcagcga cgcgcaaaac cacccggcgt
    12121 tctcgttccg cccggagact ggcgaggaaa tctatcattc attcctcggc gttccgatcc
    12181 tgcgcgccgg caacacgctc ggcgtgctgg tggtgcagaa ccgcgccaag cgcacctatg
    12241 tcgaggaaga ggtcgaggca ctgcagacca ccgcgatggt gctggcagag atgatcgcct
    12301 cgggcgaatt gtcggcgctg gcgcagcccg gcgccgagcc ggccgcccgg cattcagtgc
    12361 acaagaccgg cgcgatcctg tccgacggta tcgcgctcgg ccatgtggtg ctgcacgagc
    12421 cgcgcgtcat catcaccaat tacattgccg acgacctgcc gaaggaaatc aagcggctcg
    12481 acgcggcgct gacgaaattg cgcgccgacc tcgaccgtat gctggagcgc ggcgacgtcg
    12541 ccgagggcgg cgagcaccgc gatgtgctgg aagcctaccg gatgttcgcc aacgaccacg
    12601 gctggtcgca ccggctgcac gaagccgtcg ccacgggcct caccgccgag gccgcggtcg
    12661 agcgcgtgca atccgatacg cgcgcccgca tgctgcgctc caccgatcct tatctgcgcg
    12721 accgcctgca cgatctcgaa gacctcggcc atcggctgat gcggcagctg gtgggccagg
    12781 atcacgcgcc ttcgcgggag cagttgcccg acaacgccat cctgatcgcg cgcgcgatgg
    12841 ggccggcggc cctgctcgac tacgacagaa agagattgcg cgggctggtg ctggaagaag
    12901 gcaccgccaa ttcccacgtc tcgatcgtgg cgcgcgcgct cggaatccca gcggtcggcg
    12961 aagtgccgaa cgcgccgggc attgccgatc ccggcgatgc catcatcgtc gacggcacct
    13021 cgggctcgat ctatgtccgc ccctcggcgg aaatcgaatc agcctatgcg gaacgggtgc
    13081 ggtttcgcgc caggcggcag gcgcaatatt cggcgctgcg cgacaagccc tgcgtcacca
    13141 aggacggcca acacatcgaa ttgctgatca acgccgggct gatcatcgat ctgccgcata
    13201 tcgacgacag cggcagcgcc ggcatcggcc tgttccgcac cgaactgcaa ttcatggtgg
    13261 gccagagcct gccgcgcacc agcgaccagc tcgcgctcta ccgcgcggtg ctggatgccg
    13321 cgggcccgaa gcccgtgacg ttccggaccc tggacattgg cggcgacaaa gcactgccct
    13381 atatggaaac cgtgatcgag gaaaatcccg cgctcggctg gcgcgcgatc cggctcggcc
    13441 tcgaccggcc tgggctattg cgcggccagg tccgcgcctt gctgcgggcc ggcggcggcc
    13501 gcgcgttgcg catcatgttc ccgatgattt cggaggtcgc cgaattcgat gccggcaaaa
    13561 gcatcgtcga gcgcgaactg acctatctgc gtcagcatgg ccatgcgctg ccggaacgca
    13621 tcgacgtcgg caccatggtc gaagtgccgg cgctgctcta tcagctcgac gaactcctga
    13681 agaaagtcga tttcatctcg gtcgga
//
LOCUS       DATNOI010000632         6308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_262497,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000632 DATNOI010000000
VERSION     DATNOI010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262497"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VN968_25630"
     CDS             complement(<1..254)
                     /locus_tag="VN968_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXL32645.1"
                     /translation="MLARDWYYNERRQIGLDTAVAAIYDTDDDSDLRARAALTMLGVQ
                     RGWRVADIGCGNGVLACEAALIGAEVDAIDISPAMLRLAE"
     gene            complement(258..800)
                     /gene="ilvN"
                     /locus_tag="VN968_25635"
     CDS             complement(258..800)
                     /gene="ilvN"
                     /locus_tag="VN968_25635"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HXL32646.1"
                     /translation="MNQPASAYFMEQRHDPIEAHTLSVLVQNEPGVLARVIGLFSGRG
                     YNIESLTVSETESQKHLSRITIVTTGTPMVIEQIKHQLDRMVPVYRVVDMTLTGRSIE
                     RELAMVKLRGTGEHRVEALRLAEAFRARVIDATTESFVFEITGNSAKINQFIDLMRPL
                     GLVEVSRTGVAAIGRGAEGM"
     gene            complement(1014..2003)
                     /locus_tag="VN968_25640"
     CDS             complement(1014..2003)
                     /locus_tag="VN968_25640"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HXL32647.1"
                     /translation="MFDLQQLERAQTIVAAAMPPTPAHQWPLLSERLGATAIVKHENH
                     TPTGAFKVRGGLVYVDRLKRERPHTAGLISATRGNHGQSVAFAAGRYRVPVTIFVPRG
                     NSVEKNRAMRAFGADLVEHGEDFQAAREEAGRRAKLDGLEFVPSFHPDLVLGVATYAL
                     ELLRKAPDLDVLYVPIGQGSGICGCILARELLGLKTEIVGVQSTEAPSYALSFAAGTV
                     VTTETSNTLADGLATRVPDADALAIMRRGASRIVQVTDDEIRAAITALWTDTHNLAEG
                     AGAAALAAALQDKPKIRGKRVGLILSGGNIDFDLFNRWVVPQAAVFAGSKALV"
     gene            complement(2019..3794)
                     /locus_tag="VN968_25645"
     CDS             complement(2019..3794)
                     /locus_tag="VN968_25645"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HXL32648.1"
                     /translation="MSDKNHDPNQMTGAAMIVRALVDHGVKHIFGYPGGAVLPIYDEI
                     FQQDDVEHILVRHEQGAGHAAEGYARSTGKPGVVLATSGPGATNMVTPLTDALMDSIP
                     LVCITGQVPTHLIGTDGFQECDTVGITRPCTKHNWLVRNVNDLAKVLHEAFYVASTGR
                     PGPVLVDVPKDVQFAVGTYHPPRKSDVHVSYAPRVKGDAAQIRKAVALLASAKRPVIY
                     SGGGVINSGSEASRLLRELVDATGFPITSTLMGLGAYPASGKNWLGMLGMHGTYEANM
                     AMHGCDAMLCVGARFDDRITGRVDAFSPGSKKIHIDIDPSSINKNIRIDIPIIGDAGN
                     VLGDLLQVFKAEAKKPDIRPWWQEIAKWRARNSLSYKKNGDIILPQYAIQRLFENTRG
                     RDVYITTEVGQHQMWAAQFFGFEEPHRWMTSGGLGTMGYGLPAAVGVQVAHPDSLVID
                     IAGDASVQMTMQEMSTAVQHELPIKIFILNNQYMGMVRQWQQLLHGNRLSHTYSEALP
                     DFVKLADAFGCVGIQAIKPGDLDGAIKEMINVKRPVLFDCRVAALENCFPMIPSGKAH
                     NEMLLPAEANDEATAAAFAGGKALV"
     gene            complement(4204..5139)
                     /gene="miaA"
                     /locus_tag="VN968_25650"
     CDS             complement(4204..5139)
                     /gene="miaA"
                     /locus_tag="VN968_25650"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXL32649.1"
                     /translation="MSSVQENGNAVLIAGPTASGKSALALELAQATGGLIINTDSMQV
                     YRDLRVITARPTPEQEAVVPHRLYGHIDAAVNFSAGSWVVDAAKVLAEARAQHRLPIF
                     VGGSGLYFKALTRGLSAVPPTPAVIREDVRARLERDGVEALHAELARRDPVSAGRLKP
                     RDRTRIARALEVVEATGRSLTDWHRDGLPPLLPQGQFRALFLGPDREQLYARIDSRFE
                     TMLLSGALEEVAALAARRLDPLLPAMKAHGVPALIRHLEGELTLEEAAEIGRADTRHY
                     AKRQFTWFRHQLPEFEWVRPEEAEGWFTAVIPGRA"
     gene            5145..6038
                     /gene="serB"
                     /locus_tag="VN968_25655"
     CDS             5145..6038
                     /gene="serB"
                     /locus_tag="VN968_25655"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HXL32650.1"
                     /translation="MSLVATLICNPADPALDSTVVDGARAILPSPGPAQWLFNEVAVD
                     IPFTGSDDIQAIANRLRQARGDLPIDIVVQPRALRRKKLFLADMDSTMIGQECIDELA
                     DFAGLKAHVAGITERAMRGEIEFEPALRERVALLKGMPVSVVDEVLAKRITPTPGGRE
                     LVATMRAHGAYTCLISGGFTLFTNAVAAGIGFQESRANELKVEDGKLTGEVREPILGR
                     AAKLATLIELRESFDLDNIDTLVVGDGANDLGMIENAGLGVAYHAKPAVAAAAAARID
                     HGDLTALLYAQGYRRDEFVGV"
BASE COUNT         1133 a         2053 c         1980 g         1142 t
ORIGIN      
        1 atttcggcga gccgcagcat cgccggcgaa atatcgatgg cgtcgacttc ggcgccgatc
       61 agcgccgcct cgcaggccag cacgccgttg ccgcagccaa tatcggccac ccgccagccg
      121 cgctggacgc cgagcatggt caacgccgcg cgggcgcgga ggtcgctgtc atcgtccgtg
      181 tcgtagatcg cggcaaccgc ggtgtcgaga ccgatctgcc gccgctcgtt gtagtaccaa
      241 tcacgcgcca gcatggttca catcccttcc gcgccgcggc cgatcgcggc aacgccagta
      301 cgcgagacct cgaccaggcc aagtggtcgc atcaggtcga tgaactggtt gatcttggca
      361 gagttgccgg tgatttcaaa cacaaagctc tcggtggtgg catcgatcac gcgcgcgcga
      421 aacgcttccg ccagacgcag cgcctcaacg cggtgctcgc cagtgccgcg cagcttcacc
      481 atcgccagct cgcgctcgat cgagcggccg gtcagcgtca tgtcgacgac gcgatacacc
      541 gggaccatgc ggtcgagctg gtgcttgatc tgctcgatca ccatcggcgt tcccgtggtg
      601 acgatggtga tgcgggagag gtgtttctgg ctctcggtct ccgagaccgt gagactctcg
      661 atgttgtagc cgcggcccga gaacagcccg atcacgcgcg cgagcacgcc cggctcgttc
      721 tgcaccagca ccgagagggt gtgggcctcg atcggatcat ggcgctgttc catgaagtag
      781 gcggatgcgg gctggttcat tttaattccc tgtcgttgcc ttgttcgtcc tgcctattcc
      841 gctcgtcacc cccagcaccg ggtctcgcca atggcgagcc cgatgacagg ctccaacggg
      901 tatccggtaa tcgctggcgt acgcgataca atcgaaggcg cgagcgctac cgaatcgtcc
      961 ggtcgagccg gacgatgaca atagaattgg gtgtggctgc tccgtgacaa tcatcacacc
     1021 agcgccttgc tgccggcgaa gacggcggct tgcggaacga cccatcggtt gaacaaatcg
     1081 aaatcgatgt taccgccgct caggatcagt ccaacccgct tgccccggat tttcggcttg
     1141 tcctgcaggg ccgcggcaag cgccgcggcg ccggcaccct cggcgagatt gtgcgtatcc
     1201 gtccaaagcg ctgttatcgc cgcgcggatc tcgtcgtcgg tgacctgcac gatgcgcgag
     1261 gcgcccctgc gcatgatcgc cagcgcatcg gcatcgggga cgcgcgtcgc caagccgtcg
     1321 gccagcgtat tgctggtctc ggtcgtcacc accgtgccgg ccgcgaacga cagcgcatag
     1381 gacggcgcct ccgtcgattg cacgccgacg atttcggttt tcagcccgag cagctcgcgc
     1441 gccaggatgc agccgcagat gcccgatccc tgtccgatcg gaacatagag gacgtcaagg
     1501 tccggcgcct tccgcaagag ttcaagcgca taggtcgcga cgccgagaac cagatccgga
     1561 tggaaggacg gcacgaattc cagcccatca agcttggcgc ggcgtccggc ctcctcgcgg
     1621 gcggcctgaa aatcttcgcc gtgctcgacg agatcagcac cgaaagcccg catggcgcga
     1681 tttttttcga ccgaattgcc gcgcggcacg aagatcgtca ccggcacccg gtagcggccc
     1741 gccgcgaaag caacgctctg gccatgattt ccgcgcgtcg ccgagatcaa tccggcggtg
     1801 tgcggccgct cgcgcttcag gcgatcgaca tagacgagcc cgccacgcac cttgaaggca
     1861 ccggtcggcg tgtggttctc atgcttgaca atagccgtcg cgccgagccg ctcggacagc
     1921 agcggccatt gatgcgccgg ggtcggcggc atcgccgcgg cgacgatggt ttgggcgcgt
     1981 tccagttgct gcagatcaaa cattggctcg tctcgccctc acaccagtgc cttgccgccg
     2041 gcgaaggccg cggcggtggc ttcgtcgttg gcttccgccg gcaacagcat ttcgttgtgc
     2101 gccttgccgg acgggatcat cgggaaacaa ttttccagcg ccgccacccg gcagtcgaac
     2161 agcaccggcc gcttgacgtt gatcatctcc ttgatggcgc cgtcgagatc gccgggcttg
     2221 atcgcctgga tgccgacgca gccgaaggcg tccgcgagct tgacgaagtc cggcagcgct
     2281 tccgaatagg tgtgcgacag ccggttgccg tgcaggagct gctgccactg ccgcaccatg
     2341 cccatatact ggttgttcag gatgaagatt ttgatcggca gttcatgttg aacggccgtc
     2401 gacatctcct gcatcgtcat ctgcaccgag gcgtcgccgg cgatgtcgat caccagactg
     2461 tcgggatgcg cgacctggac gccgaccgcc gccggcaatc catagcccat ggtgccgaga
     2521 ccgcccgagg tcatccagcg gtgcggctcc tcgaagccga agaattgcgc cgcccacatc
     2581 tggtgctggc cgacttcggt ggtgatgtag acgtcgcggc cgcgtgtatt ctcgaacagc
     2641 cgctgaatgg cgtattgcgg caggatgata tcgccgttct ttttatacga cagcgaattg
     2701 cgcgcgcgcc atttcgcgat ctcctgccac cacggccgta tgtccggctt tttcgcctcc
     2761 gccttgaaca cctgcaacag atcgccgagc acattgccgg catcgccaat gatcggaatg
     2821 tcgatgcgga tgttcttgtt gatcgaggag ggatcgatat cgatgtggat cttcttcgag
     2881 cccggcgaga atgcgtcgac acgtccggta atgcggtcgt cgaagcgcgc gccgacgcac
     2941 agcatcgcgt cgcagccgtg catcgccatg ttggcctcat aggtgccgtg catgccgagc
     3001 atgccgagcc agttcttgcc cgacgccgga taggcgccga gacccatcaa ggtggaggtg
     3061 atcggaaacc cggtggcgtc gaccagttcg cgcagcaacc ttgaggcttc ggagccggaa
     3121 ttgatgacgc cgccgccgct atagatcacc gggcgcttgg ccgaggccaa taacgccacg
     3181 gctttgcgga tctgcgccgc gtcgcccttc acccgcggcg cgtaggacac atgcacgtcg
     3241 gacttgcgcg gcgggtgata ggtgccgacc gcgaactgca cgtccttcgg tacatcgacc
     3301 aggacagggc cggggcgtcc ggtgctggcg acatagaacg cctcgtgcag caccttggcc
     3361 agatcgttga cgttgcgcac cagccagttg tgcttggtgc agggccgggt gatgccgacg
     3421 gtgtcgcatt cctggaaccc gtcagtgccg atcaggtggg tcggcacctg cccggtgatg
     3481 cacaccagcg ggatcgaatc catcagcgcg tcagtcagcg gcgtcaccat gttggtggcg
     3541 cccggccccg acgtcgccag caccacgccg ggcttgccgg tggagcgcgc gtagccctcg
     3601 gcggcgtgac cagcgccctg ctcgtgccgc accaggatgt gctcgacgtc atcctgctgg
     3661 aaaatctcgt catagatcgg aagcaccgcg ccgcccggat aaccgaagat gtgcttgacg
     3721 ccatgatcga ccagcgcgcg cacgatcatg gctgcgccgg tcatctggtt cggatcgtgg
     3781 ttcttgtcgc tcatggctgg ctccggatac gctttgctgc gcggcttctt cagttgggtt
     3841 tttggaaaat aaaaaggggc ccaagaggcc ccatgcacac cgcccgaatt tggatggcct
     3901 cagccatccc cggcggtgcg cctgggtacg acaacgataa ggagcctggt aattttatta
     3961 cgcatatgac cgctcggact tctcgaagat tgcgcaaacc ataacgacca taccccaaaa
     4021 gtcaaggcaa gcggccatga agcactgaat ttgaagctta gccgggttcc ggtcgttcgg
     4081 cgagggtaaa ttttgcgttt gcagccatgt gcgctccctc gaccgtcatt ccgggggcgc
     4141 gcgaaagcgc gaacccggaa tctcgagatt ccccgatgtg caattgcaca tctgaggttc
     4201 gcgctacgcg cgccccggaa tgacggcggt gaaccatccc tccgcctcct ccggcctcac
     4261 ccactcgaat tccggcagct gatgccggaa ccaggtgaat tgccgtttgg cgtaatgcct
     4321 ggtatcagcg cggccgatct cggccgcctc ttcgagcgtg agctcgccct cgagatgccg
     4381 gatcagcgcc ggcacgccat gggccttcat cgccggcagc agcggatcga gccgccgcgc
     4441 tgccaaggcg gccacctctt ccagcgcgcc tgacaaaagc atcgtttcga aacgggagtc
     4501 gatgcgcgca taaagttgct cgcgatcggg gccaagaaac agcgcgcgaa attgaccctg
     4561 tggcaacagc ggcggcaatc cgtcgcgatg ccaatcggtc agcgaacgcc cggtggcctc
     4621 gacaacttcc agcgcgcggg cgatgcgggt gcgatcgcgc ggtttcaagc gcccggcgga
     4681 cacaggatcg cgccgcgcga gttcggcatg cagcgcctcg acgccatcgc gttcgagccg
     4741 cgcgcgaacg tcctcacgta tcacggcagg agtaggcggc accgccgaga gcccacgtgt
     4801 caacgccttg aagtaaagac ctgacccgcc aacaaaaatc ggcaggcgat gttgcgcgcg
     4861 cgcctccgca agcactttcg cggcatcgac cacccagctg cccgccgaga aattcacggc
     4921 ggcatcgatg tggccataga gccggtgcgg cacgaccgcc tcctgttccg gcgtcggccg
     4981 cgccgtgatg acgcgcagat cgcggtatac ctgcatggaa tcggtgttga tgatgaggcc
     5041 gccggtcgcc tgtgccagct cgagcgccag cgccgacttg ccgctggcgg tcggccctgc
     5101 gataagcacg gcgttgccgt tttcctgaac cgaactcacg tcaaatgtct ctcgtcgcca
     5161 cgctgatctg caatcccgct gaccccgcgc tcgatagcac ggtggtggac ggcgcgcgcg
     5221 ccattcttcc atcgcccggc ccggcgcaat ggctgttcaa tgaagtggcg gtcgatatcc
     5281 ccttcacggg cagcgatgat atccaggcca tcgccaaccg cctgcgtcag gcgcgcggcg
     5341 acctgccgat cgacatcgtg gtgcaaccgc gggcgttgcg gcgcaagaag ctttttctcg
     5401 ccgacatgga ttccaccatg atcggccagg aatgcatcga cgagcttgcg gacttcgccg
     5461 gcctgaaggc gcatgtcgcc gggattaccg agcgcgcgat gcgcggcgaa atcgagttcg
     5521 aaccggcgct gcgcgagcgc gtcgcgctgt tgaagggcat gcccgtcagc gtggtcgatg
     5581 aagtgctggc aaaacgcatt acgccgacgc caggcggccg cgaattggtc gcgaccatgc
     5641 gcgcccacgg cgcctacacc tgcctgatct cgggcggttt tacattgttc accaatgcgg
     5701 tcgcggcagg aatcggcttt caggaaagcc gcgccaatga attgaaggtc gaggacggca
     5761 agctgaccgg cgaagtcaga gagccgatcc tgggccgcgc ggcaaagctc gcaaccctga
     5821 tcgagctgcg cgaatccttc gatctcgaca acatcgacac gctggtagtc ggcgacggcg
     5881 ccaacgatct cggcatgatc gagaatgccg gcctgggggt cgcctaccac gccaagccgg
     5941 ccgtcgccgc ggccgccgcc gcaaggatcg atcacggcga tctcaccgcg ttgctctatg
     6001 cgcaggggta tcggcgggac gagtttgtgg gggtgtagta cccggatgtt cagcagtcat
     6061 gcccggactt gatccgggca tccatcagac ttcaaaaaga accttgcgaa gaggatggat
     6121 tgccgggtca agcccggcaa tgaccatagg gggatgtctt gaccagaccc tactgcacgc
     6181 tcagcgccac gaagcgcaat tcgccgtcgg cgttggacac cagcagcagg acggatttct
     6241 tgccgtcctt cttcagctga tcgacgcgct tcttgacgtc ggcggcgttg gccaccgcct
     6301 cctgcgcc
//
LOCUS       DATNOI010000633         8548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_73769,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000633 DATNOI010000000
VERSION     DATNOI010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73769"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VN968_25660"
     CDS             <1..883
                     /locus_tag="VN968_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HXL32651.1"
                     /translation="WDADEPLVLPPIYLFGVWLSILLAIGVTSLYAFQVTQEARKLSD
                     ALAATELVLTREQHLTQLDGLAAAAAHELGTPLSTIFLISRELEQSVPADSPIAGDIK
                     TLREQAQRCRDILAKITQLSATGAPFDRMPLSTLIEETVAPYRDFGVAIKVRIAVAGT
                     SEPVGTRNPAILHGVGNILENAVDFARTTAEVNAWWNTETIEIVISDDGPGFAPDILK
                     RIGEPYLSRRRSIDEAQSEHGGLGLGVFIARTLLERTGARVSFTNRTFPDHGAVVQIA
                     WPRRRFEAGETAPEPAG"
     gene            990..1544
                     /locus_tag="VN968_25665"
     CDS             990..1544
                     /locus_tag="VN968_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HXL32652.1"
                     /translation="MNAIPELTAHADRSLLIVEDDKPFLERLSRAMETRGFTVTSCDS
                     VSEGLAQIGKAPPAFAVVDLRLGDGNGLDVVSALKRRRPDARTIVLTGYGNIATAVTA
                     VKMGAVDYLSKPADADDVVAALLASGTEKSEPPHNPMSADRVRWEHIQRIYEMCNRKV
                     SETARRLNMHRRTLQRILAKRAPR"
     gene            complement(1552..2037)
                     /locus_tag="VN968_25670"
     CDS             complement(1552..2037)
                     /locus_tag="VN968_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HXL32653.1"
                     /translation="MESPARQVSLVPPPDRRQSETALAVARGTARLLRSLGFSCVSEL
                     PLPSGRRADLVALNERGEIWIVEIKSSIEDLRADQKWQDYRAHCDRLFFAFTQDLPCE
                     IFPADTGLIVADAYGAHMHCEAPEHRLPAPTRKLMTVRFAIAAAQRINRLVDPQGHGE
                     F"
     gene            2334..4034
                     /locus_tag="VN968_25675"
     CDS             2334..4034
                     /locus_tag="VN968_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HXL32654.1"
                     /translation="MIDDLWYKNGVFYCLSVGTYMDANADGVGDFTGLLRRLDYLHGL
                     GITTIWLMPFQPSPFRDDGYDISDYYGVDSRYGTLGDFIEFTHGCQQRGMRVIIDLVV
                     NHTSDQHPWFRDARKSKASPYRDWYVWSDKKPPNASKGMVFPGVQKSTWTYDKEAKAW
                     YFHRFYDFQPDLNTSNPYVQAEILKIMGFWIQLGVSGFRMDAVPFVISTKGPKVRKPV
                     EQYDMLRSFREFLQWRQGDAIILGEANVLPKTDMEYFGKAGERIQMMFNFHVNQHLFH
                     ALASADTRPLAKALVATKPRPATAQWGLFLRNHDELDLGRLTKAQRELVFKEFAPDKN
                     MQLYDRGIRRRLAPMLGGDRRRLELAYSLLCTLPGTPVIRYGDEIGMGDNLDLPERNC
                     ARTPMQWSTEPHAGFTESDKPMLPVIDKGPYGYEHVNVAKQRRDPNSLLNWTERIIRM
                     RKEVPEIGWGDFKVIATRDAAVLVIRYDWRNNSVLFVHNLDEKPREVSFAVGLAGEAG
                     KLLVNLLSEDHSNASEAGRHTLLLEAYGYRWYRVGGLDYLLKRSDIDADAVGGTHHPA
                     "
     gene            complement(4184..5800)
                     /locus_tag="VN968_25680"
     CDS             complement(4184..5800)
                     /locus_tag="VN968_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXL32655.1"
                     /translation="MNQHDKLWWRHGIFYQIYPRSFQDTNADGVGDIKGIIERLPYLQ
                     SLGVDAIWLSPIFPSPMADFGYDIANYTGIDPLFGTMGDFDALVYTAHASGLKIILDL
                     VPNHTSDQHPWFVESRGSCDNAKRDWYIWRDPAADGGPPNNWLSEFGGSAWQYDPTTR
                     QYYYHAFLAQQPDLNWRNPEVRRAIYDVMRFWLRKGVDGFRVDVIWHLVKDAEFRDNP
                     PNRDYREGRPPHERILPLNSTDQSEVQDVIADMRRVIDEFDSRVLIGEIYLPLERLVV
                     YYGKDLAGAHLPFNFALLSAPWNARAIEKIIFKCESVLPQGAWPNWVLGNHDRPRVAS
                     RVGRAQVRVAAMLLLTLRGTPTLYYGDEIGMHQVAIAPEQVHDPFEKNVPGIGVGRDG
                     CRTPMQWDATRYAGFSTSRPWLPLADDFAHENVVNLNADAASILSLYKALIGLRKKLP
                     QLVSGDYVPVAAEGDLLLYRREGAEGTAVIALNLGAEPVSIKSDAAGLNGEILLSTFL
                     DRQGEKIQGALDLRGDEGVIIGASSVAQPV"
     gene            6054..6722
                     /locus_tag="VN968_25685"
     CDS             6054..6722
                     /locus_tag="VN968_25685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL32656.1"
                     /translation="MTQEAETKAKAGAIIVPVTLFEQNCTLIWCEATRKAVVIDPGGD
                     VPKIQAAIKQSNVSVEKIWLTHGHIDHVGGAAELRDALKVPIEGPHRTDKFLLDNVVM
                     SGARFGITGVRDFAPDRWLNEGDQVSIGELTFDILHCPGHSPGSVVYFSNEMRFAHVG
                     DVLFNGSVGRSDIPGGNHSTLIKSIKDKLLPLGDDVAFICGHGPGSSIGHERMTNPFL
                     TGAM"
     gene            complement(6788..7165)
                     /locus_tag="VN968_25690"
     CDS             complement(6788..7165)
                     /locus_tag="VN968_25690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL32657.1"
                     /translation="MAKAKSALTCGCPVAAFQKMISGKYKLRIVWDLKDGPRRYGEIR
                     TGLLSGSDGTSEIAPRVLSRELKALTESGLIERRDYGVVPPKVEYRLTRRGKSFVPVI
                     AAIRDWGARHLAQANPMEMVAAE"
     gene            7320..8201
                     /locus_tag="VN968_25695"
     CDS             7320..8201
                     /locus_tag="VN968_25695"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL32658.1"
                     /translation="MILVTGATGLNGKEVLRQLSARGVAVRALVRNPAKAAALAALPN
                     VEIVQGDMARGETLALALHDIDRAMLISSSAPDMLEVQSNFIDAARKAGVKHVVKLSG
                     IMPELDSAFRFARMHGEIEKRLEASGMAFTHLRAGEFMPAYFRQVPAIVAKGAMFLPM
                     EDARIASIDVGDIAEIAADVLTHSGHEGKTYPLTGPEALTMTQVAEKLSAATGKTIRY
                     VDVPPEAARQAQLGAGMPPYLADALFELFAERRNGKEARVWPDAGALLGRPPTSFDEF
                     ARRNAAVFRGEAPPPQV"
     gene            8235..8537
                     /locus_tag="VN968_25700"
     CDS             8235..8537
                     /locus_tag="VN968_25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32659.1"
                     /translation="MKHFMIKYQFANGTTEDWHREIGRFIVALDNDPELKGKITYRCM
                     KNRDDTSYYHLAADADEQAIKTLQQRDFFKHYTEKTRQVAGGEVVVTPIELIAETA"
BASE COUNT         1715 a         2674 c         2590 g         1569 t
ORIGIN      
        1 gtgggacgcc gatgagcccc tggtattgcc gccgatctac ctgttcggcg tctggctctc
       61 gatcctgctg gcgatcgggg tcaccagtct ttatgcattc caggtgaccc aggaggcccg
      121 caagctctcg gacgcactgg cggccaccga attggtattg acccgtgaac agcatcttac
      181 ccaactggac ggtctggccg ccgctgccgc gcatgagctc ggcacgccgc tgtcgacgat
      241 ctttttgata tcgcgcgagc tggagcagag tgtgccggcg gacagcccga ttgccggtga
      301 catcaagacc ttgcgcgaac aggcgcaacg ctgccgcgat atcctggcca agatcacgca
      361 gctgtccgca accggcgcgc cgttcgaccg catgccattg tcgaccctga tcgaggaaac
      421 ggttgcgccg tatcgcgatt tcggcgtcgc gatcaaggtc cggattgcgg tagcgggcac
      481 aagcgagccg gtcggcacgc gtaatccggc aatcctccat ggtgtcggca acatcctgga
      541 aaacgccgtc gatttcgcgc ggacgacggc ggaggtgaac gcgtggtgga acaccgagac
      601 catcgagatt gtcatttccg acgatggccc gggtttcgcg cccgatattt taaagcgtat
      661 cggcgaaccc tatttgtcgc gccggcgcag catcgacgaa gcccagagcg agcacggcgg
      721 cctcggtctc ggggtgttta tcgcccgcac gctgctcgag cgcaccggcg ccagggtctc
      781 cttcaccaac cggacctttc cggatcatgg cgccgtggtt cagatcgcct ggccgcgccg
      841 ccgcttcgag gccggtgaaa ccgctcccga accagccggc tagttcaagg cccgggacaa
      901 cgaacggagc gatcgacttg atgcggcctt ggcagcgccg cgttgctacg ccatatgttg
      961 cagtgctcga caaggaggat atccgaatcg tgaatgccat ccccgaactg accgcccacg
     1021 ccgatcgctc gctgctgatc gtggaggacg acaagccgtt tctggagcgc ctgtcgcgcg
     1081 ccatggaaac ccgcggcttc acggtgacat cctgcgacag cgtgtccgaa ggtctggcgc
     1141 agatcggcaa ggcaccgccg gcgttcgcgg tagtggattt gcggctcggc gacggcaatg
     1201 gccttgacgt ggtatcggcg ctcaagcgca ggcgccccga tgcgcgcacg atcgtgctga
     1261 ccggctatgg caacattgcc accgccgtga cggcggtgaa gatgggcgca gtggattatc
     1321 tctccaagcc cgccgacgcc gacgatgtgg tcgccgcctt gctcgcgagc ggcaccgaga
     1381 agtccgagcc gccgcacaat cccatgtcgg cggatcgggt acgctgggag cacattcagc
     1441 gcatctatga aatgtgcaac cgcaaagtct ctgaaacggc gcggcggctg aacatgcatc
     1501 gccgcaccct gcagcgcatt ttggccaagc gagcaccgcg gtaacgttgc cctaaaactc
     1561 cccgtggccc tgcggatcga ccagccgatt gatccgctgc gcggcggcga tcgcgaaccg
     1621 caccgtcatc agcttgcggg tcggcgccgg tagccgatgc tccggcgcct cgcaatgcat
     1681 atgcgcgcca taggcgtccg cgacgatcag acctgtatcc gcgggaaaaa tctcgcacgg
     1741 caaatcttgc gtaaaggcga agaacagccg gtcgcaatgc gcccggtaat cctgccactt
     1801 ctggtcggcc cgcaaatcct cgatcgatga cttgatctcg acgatccaga tctcgccgcg
     1861 ctcgttcagt gctaccaggt cggcgcgccg tcccgagggc agcggcaact cgctgacgca
     1921 ggaaaatccc agcgaccgca acagccgcgc ggtgccgcgc gcgaccgcca gcgccgtctc
     1981 cgactgacgg cgatccggcg gaggcacgag gcttacctgg cgggcgggcg attccattga
     2041 aacaaagcct agccgattcc gcagcggcgc tgaagccgca gatcgtggag caagctggcg
     2101 gagcgttctc cgagccgtcc ccgcgaaagc gggacccatg accaccattg cgcgttgcta
     2161 cacctgctgg gggctccaac ctactgcaac accaggattg gtggttatgg gtctcggctc
     2221 gcgctttgcc tggccggggc gacgcagtct tatcggctgg aaccctgaaa gcctttgagg
     2281 cttataaaat ggccgctgat gacgcgaggc ggaccgaaga gggctgcctg cggatgatcg
     2341 acgacctatg gtacaagaat ggcgtcttct attgtctgtc ggtcggcacc tatatggacg
     2401 ccaatgccga tggcgtcggc gacttcacgg ggctattgcg ccggctggac tacctgcacg
     2461 ggctcggaat caccacgatc tggctgatgc cgttccagcc gtcgcccttc agagacgacg
     2521 gctacgatat ctcggactat tacggcgtcg attcgcgata tggcacgctc ggcgacttta
     2581 tcgagttcac ccatggctgc cagcaacgcg gcatgcgcgt catcatagac ctcgtcgtca
     2641 atcatacatc ggatcaacac ccctggtttc gcgacgcccg aaagtccaag gcttcgccgt
     2701 atcgcgattg gtatgtctgg tccgacaaga agccccccaa tgccagcaag ggcatggtgt
     2761 ttccgggcgt gcaaaaatcc acgtggacat acgacaagga agcgaaagcc tggtacttcc
     2821 atcgcttcta tgacttccag cccgacctca acacgtcaaa cccgtatgtg caggccgaga
     2881 tcctcaagat catgggcttc tggattcagc tcggcgtctc cggattccgg atggatgcgg
     2941 tgccattcgt catctccacc aagggaccga aggtccgcaa acccgtcgag caatacgaca
     3001 tgctgcgctc gttccgggaa tttctgcaat ggcggcaagg cgacgccatt attcttggcg
     3061 aagccaatgt gctgcccaaa accgacatgg aatatttcgg caaggcgggc gagcgcatcc
     3121 agatgatgtt caactttcac gtcaaccagc acctgttcca tgcgctagcc tccgccgaca
     3181 cccgcccgct ggcgaaggcg ctggtggcga cgaagccccg ccccgcgacc gcgcaatggg
     3241 gccttttcct gcgcaaccac gacgaactcg atctcggcag attgaccaaa gcgcagcgcg
     3301 aactggtgtt caaagaattc gcccccgaca agaacatgca gctctacgac agggggatcc
     3361 ggcgccggct cgcaccgatg ctgggcggcg accggcgccg cctcgaactt gcctacagct
     3421 tgctgtgcac gctgccggga acgcccgtga tccgttatgg cgacgaaatc ggaatgggcg
     3481 acaatctcga tctgcccgag cgcaattgcg cccgaacgcc gatgcaatgg tcgaccgagc
     3541 cgcatgccgg attcacggaa agcgacaaac cgatgttgcc ggtgatcgac aaggggcctt
     3601 acggatacga gcacgtcaat gtcgccaagc agcgccgcga tcccaactcc ctgctgaact
     3661 ggaccgaacg catcatccga atgcgaaagg aagtaccgga aatcggctgg ggtgacttca
     3721 aggtaatcgc aacgcgcgat gcggccgttt tagtcattcg ctatgattgg cgcaataact
     3781 ccgtgctgtt cgtgcacaat ctcgacgaga agccgcggga agtctcgttt gctgtcgggc
     3841 ttgccggaga ggccggaaaa cttctggtca acctgctatc ggaagatcac agcaacgcta
     3901 gcgaagccgg caggcacacg ctgctgctcg aagcgtacgg gtatcgctgg tatcgtgtcg
     3961 gaggcctcga ttatctcctg aagcgcagcg acatcgacgc cgacgcggta ggcgggacac
     4021 atcacccggc gtgagaagtg ttcgatgagc acgcgcctct gtgagtcgtc cccgcgaaag
     4081 aaagcgtagc ccggatggag cgaagcgaaa tccgggacct tctcgcaagc gggtaaactt
     4141 atcccggatt gcgcttcgct ccatccgggc tgccaggctg cgttcacact ggttgggcga
     4201 cggacgatgc accgatgatc acgccctcgt cgccgcgcaa atccaacgcg ccctggatct
     4261 tttcgccctg tcgatcgagg aaggtcgaaa gcagaatttc gccgttgagg cccgccgcat
     4321 ccgacttgat cgaaaccggt tccgctccca ggttcagcgc gatcacggcc gtgccctcgg
     4381 cgccctccct gcggtaaagc aacaaatctc cctcggcagc aacaggcacg tagtcgccgg
     4441 acacgagttg cggcaatttc tttcgcaagc cgatcagcgc cttgtagagg ctcagaatcg
     4501 aggcggcgtc ggcgttgaga ttgaccacat tttcgtgggc aaaatcatcg gccagcggca
     4561 gccatggcct ggatgtcgag aagcccgcat aacgcgtcgc gtcccattgc atcggggtgc
     4621 ggcagccgtc gcggccgacg ccgatccccg gaacgttctt ctcgaaggga tcatgcacct
     4681 gctccggcgc aatcgccacc tgatgcattc cgatctcgtc gccgtaatag agcgtcggcg
     4741 ttcctcgcaa ggtcaataac agcatcgcgg cgacgcgcac ctgcgcccgc cctacccggc
     4801 ttgccacccg cggacgatca tgattgccca gcacccagtt cggccacgcg ccttgaggca
     4861 ataccgactc gcatttgaag atgattttct cgattgcccg cgcgttccat ggcgccgaca
     4921 gcagcgcaaa gttgaacggc agatgcgcgc ccgcaagatc cttgccgtaa tagacgacga
     4981 gccgttcgag cggaagatag atttcgccga tcagcacccg cgaatcgaat tcatcgatca
     5041 cgcgccgcat atcggcgatc acgtcctgga cttcggactg gtcggtggaa tttagcggca
     5101 gaatcctctc atgcggcggc cggccctcgc ggtagtcccg attgggcgga ttgtcgcgaa
     5161 actccgcatc cttgaccaaa tgccagatca cgtcgacccg gaaaccgtcg acgcccttgc
     5221 gaagccagaa gcgcattacg tcatagatgg cccggcgcac ttccgggttg cgccagttca
     5281 gatccggctg ctgggcaaga aatgcgtgat agtaatattg ccgcgtggtg gggtcgtatt
     5341 gccaggcgct gccgccgaat tccgacaacc agttgttcgg cgggccgcca tccgccgccg
     5401 gatcgcgcca gatgtaccag tcgcgctttg cattatcgca cgaaccccgg ctttcgacga
     5461 accagggatg ctggtcggag gtatggttgg gcacgaggtc gaggatgatc ttgagcccgc
     5521 ttgcatgcgc ggtgtatacc agcgcgtcga aatcccccat cgtgccgaac agcggatcga
     5581 tgccggtata attggcgatg tcgtacccga aatcggccat cggcgaggga aagatcggcg
     5641 acagccagat cgcgtcgacg ccgagcgatt gcagataagg cagccgctcg atgatccctt
     5701 tgatatcgcc tactccatca gcattggtgt cctgaaacga gcgcggatag atttggtaga
     5761 aaatgccgtg ccgccaccac aatttgtcgt gctgattcat tttggaaaat cattctttga
     5821 gatcgcgtgc ccaaggtaac gccgcacccg ccgacaggtt caggccagcg aatccctgga
     5881 gcgcacctcg catgacaccg gcgacaccgc catgacaagg cggacaagac cgcgaaaaag
     5941 cgccgcggat tgtgcctccg ggacctagcc ggaacccgcg agcgatccta tctcgatcaa
     6001 attgcttcaa tcttgccctt ggcggggcgt aaaaaaccgg cattgtagtg aacatgactc
     6061 aggaagccga aaccaaggca aaagctggcg ctatcatcgt cccggtgacg ctgtttgagc
     6121 agaactgcac cctgatctgg tgcgaagcca ccaggaaggc cgtggtgatc gaccccggcg
     6181 gcgacgttcc gaagattcag gcggcgatca agcaatccaa cgtctccgtg gaaaaaatct
     6241 ggctgaccca cggccacatc gaccatgtcg gcggcgcggc cgagttgcgc gacgcgctga
     6301 aggtgccgat cgaagggcca cacaggacgg acaaattcct gctcgacaat gtggtgatga
     6361 gcggggcacg gttcggcatc acgggcgtac gcgatttcgc gccggaccgc tggctcaatg
     6421 agggcgatca ggtctcgatc ggcgagttga ccttcgacat cctgcattgc cccggccatt
     6481 cgcccggcag cgtggtgtat ttcagtaacg aaatgcgttt tgcgcatgtc ggggacgtgc
     6541 tgttcaacgg ttcggtcggc cgcagtgaca ttcccggcgg caatcactcc accctgatca
     6601 agtcgatcaa ggataagctg ttgccgctcg gcgacgacgt cgctttcatc tgcggccacg
     6661 gcccgggttc cagcatcggc cacgagcgga tgaccaatcc gtttctcacc ggcgcgatgt
     6721 gacgatcggg ttggcgatcg cctccgtcgg ctcggcaatt tgcgcgatgc tttgattgcg
     6781 acaccttcta ttcggccgcg accatttcca tcggatttgc ctgcgcgagg tgacgcgcgc
     6841 cccagtcgcg aatcgcggca atgaccggaa caaaactctt gcccctgcgg gtcaggcgat
     6901 actcgacctt ggggggaacg acgccgtaat ccctgcgctc gatcagcccc gattcggtga
     6961 gcgccttcaa ttcgcgcgag agcacgcggg gtgcgatttc ggaagtgccg tccgagccgc
     7021 tgagcaggcc ggttctgatc tcgccatagc gtctggggcc atccttcaga tcccagacga
     7081 tccgcagctt gtacttgccg ctgatcatct tctggaacgc tgcgaccggg cagccgcagg
     7141 tcagcgctga tttcgccttc gccatggcat cacctctgat aatccgtttc gttgccgctt
     7201 ggcaggatac gagcaggcgc aagaaagtcc atagtatcat ttttgtccat acttgcggaa
     7261 tcgctcctga cagcgccaaa tcgcccccag ccgccgggag aggccacagg agacggacca
     7321 tgatcctcgt gacaggcgcc accggactga acgggaaaga ggtgctgcgc cagctgtcgg
     7381 cgaggggtgt tgccgtgcgc gcgctggtgc gcaatccggc caaggccgcg gcgctcgcag
     7441 cgctgccaaa tgtcgagatc gtgcagggcg acatggcgcg cggcgagacg cttgcgcttg
     7501 ctttgcatga tatcgaccgc gcgatgctga tttcctcgtc ggcacctgac atgctcgagg
     7561 tccagtcgaa cttcatcgat gcggcaagga aggccggggt gaagcacgtc gtcaaactct
     7621 ccgggatcat gccggagctc gattcggcat ttcgcttcgc ccggatgcac ggcgagatcg
     7681 agaaaaggct ggaagcctcc ggcatggcgt tcacccatct gcgggccggc gagttcatgc
     7741 cggcctattt tcggcaggtc cccgccatcg tcgccaaggg cgcgatgttc ctgccaatgg
     7801 aagatgccag gatcgcctcg atcgatgtcg gcgatatcgc cgaaatcgcc gctgacgtgc
     7861 tgacccattc ggggcacgag ggcaaaacct acccgctcac cgggccggag gcgctgacga
     7921 tgacgcaagt cgcagaaaaa ctttcagccg ccaccggaaa gaccatccgc tatgtcgatg
     7981 tcccgccgga ggctgcgcgg caggcccagc ttggcgctgg aatgccgccc tatctggccg
     8041 atgccttgtt cgaattgttc gccgagcgac gcaatggcaa ggaggcaagg gtatggcccg
     8101 acgccggagc gctgcttggg cggccgccga cctcgtttga cgaatttgcc aggcgcaacg
     8161 ccgcggtgtt ccggggcgaa gcgccgcctc cacaggtctg atttacgccg atctgagcag
     8221 ccaaggagcc accgatgaaa catttcatga tcaaatatca gttcgccaac ggcaccaccg
     8281 aggactggca tcgggagatc ggccggttca ttgtcgcgct cgacaatgat cccgagctga
     8341 agggcaagat cacctaccgc tgcatgaaga accgcgacga tacgagctat tatcatctcg
     8401 ccgcggacgc cgatgagcag gccatcaaaa ctttgcagca gcgggatttc ttcaagcatt
     8461 ataccgaaaa gacccggcag gtcgccggcg gcgaggtcgt ggtgacgccg atcgagctga
     8521 tcgcggaaac ggcgtgagcg aggggtaa
//
LOCUS       DATNOI010000634         8238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_74045,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000634 DATNOI010000000
VERSION     DATNOI010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74045"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="VN968_25705"
     CDS             complement(<1..1234)
                     /locus_tag="VN968_25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HXL32660.1"
                     /translation="MEAKFQIFLILLALLAGAALLARRINVAPAILLLLTGIALAFVP
                     GMPSLELPPELVLLVVLPPLIYSASVAMSWREFKHNLRPIILLSVGCVIFTAFTVAAA
                     THYLIGLPWNVGFLLGAIVAPPDVVAPLAIARKLGMPRRILVILEGEGLANDATALIL
                     YRFAVAAISTGMFSLPKAAGTFVVIVAGEVLFGAAVGWLSLRMRHRARDPQVEITLSL
                     ITPYIAYWIPEHFGGSGVISTVACGLYMSWNGPLLISSATRLQGIFFWDLVIYLIEGL
                     LFLLTGFQMRLLFEKSKAFPLDDILFATALVAIIVIVARFAWVFPATYLPRLLNKRRR
                     ARDPMPSWRTVFVIAFTGVRGAVSLAAALALPLALPGGEDFPYRDLILFVSFGVIFIT
                     LVGLGLGLPPVVRWLGVAQ"
     gene            complement(1300..1674)
                     /locus_tag="VN968_25710"
     CDS             complement(1300..1674)
                     /locus_tag="VN968_25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXL32661.1"
                     /translation="MSHDRSTVEAVVQHYFDGLYEGSADKLGAIFHPSADLRWLEKGE
                     LQVLTVPDWLDRVRKRPSAKAEGKPREDFIVTIDRSDESTAFIKVRCQLPPRYFTDYL
                     VAMKLADGWQIVSKSYRYDMRE"
     gene            complement(1780..2829)
                     /locus_tag="VN968_25715"
     CDS             complement(1780..2829)
                     /locus_tag="VN968_25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXL32662.1"
                     /translation="MRGVVFTGERGLELMQFPDPTPDAHDVVIEMKASGMCGSDLHQY
                     RRPKGQTQATGIPMKDGPVIAGHEPCGVVVAAGTAVDAKQAHIGQRVMVHHYQGCTQC
                     NHCRSGWQQLCQEVPVKVYGNNAHGGHAHYLKVPANTLVPLPDELSFSAGAAIACGSG
                     TAYSALRRMNLSGNDTLAVFGQGPVGLAATQYAKAMGARVIALDISQQRLERAKAFGA
                     DEVINPGSNDPVAAIKDLTHGRYADLTLDTSSNPDARLNAIRSTKVWGTMCFVGEGGD
                     VHIDVSPHLLRRQLTLVASWTFSNIIQAECARFSVERGIDVDKLFTHHWSIDQADEAY
                     KLFDRQVDGKGVFLM"
     gene            3074..4972
                     /locus_tag="VN968_25720"
     CDS             3074..4972
                     /locus_tag="VN968_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXL32663.1"
                     /translation="MNTHRYLKLRKIFLAAALLLMCQPAWAEKRVALVLGNSAYQNVA
                     QLPNPVNDGAIIAAKLREAGFDVVDSRHDLPAAETRRALRDFADRSRDADIAVVYYAG
                     HGMEVDGTNYLIPVDAKLERDTDVYDEAFSLDRVLLAIEPAKRLRLVILDACRDNPFS
                     KSMKRTLASRAIGQGLAKIEPSSPNMLIAYSAKAGSTAADGDGKNSPFTVALSKYLTE
                     PGLDVRRAFGFVRDDVLKNTSNRQEPFVYGSLGGEDVPLVPAPAKAAAPSTPAPSPQA
                     EARRDYELALQVGNKDAMSAFLAQYPNGFYANLAKIQLGKLAAEEARLAATEKARLAE
                     QERARLAAEGTQKAQLKAAEADAKAAEQARIAAERAKQVAQEKAAEAERKRVATETPA
                     TDKGPEIITAAGNPASDRAAASSPAAEKPQTEKAVNVAALSSGPPPAELTKPVQTADL
                     TKSVQTELRRVGCLSGDANGEWNAASQRSMALFNRNAKTKFDVKVASVDALDAIKLKP
                     ARVCPLICQHGFEADGDHCSKIVCGEGSFLNDANECEKRREKTPTARRDRDGRPDRSE
                     RLARPERLDLPVRPRPAPEVRVSRPQASGQIVCDAGGCRPVRRGCRIDYQGGSPRSGS
                     GGNEEVCN"
     gene            complement(5091..5777)
                     /locus_tag="VN968_25725"
     CDS             complement(5091..5777)
                     /locus_tag="VN968_25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HXL32664.1"
                     /translation="MAGKETEDPLLVRFRKMSKPVRVVYARPRLFASVAIGIVAFFLL
                     PGSLRLVTRLLISWDIFVTVYLVLAYVMMFRCDHSHIRRNAVLQDDGRFLILLVTALG
                     AFASIGAIVFELGASHRSVPELALATVTIALSWSAVHTIFALHYAHDYYRGAKPGGLQ
                     FPSGDEHDHADYWDFVYFSFVIGMTAQVSDVGITDKTIRRTATAHGIISFVFNTALVA
                     LMVNIAASAI"
     gene            complement(5786..6712)
                     /locus_tag="VN968_25730"
     CDS             complement(5786..6712)
                     /locus_tag="VN968_25730"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HXL32665.1"
                     /translation="MTTGTAPSDFAKRLDQTAEDTETLLAELLSDTLLPDEIARPKRL
                     IEAMRYSSLGGGKRLRPFLVVESSAVFGVARKAALLAGAALECIHCYSLIHDDLPAMD
                     NSDLRRGRPTLHKAYDDATAILAGDALLTLAFDIISRDEIHREASVRLLLTRALARAS
                     GVGGMVGGQILDLAGEGRFGDREPVDVARLQQMKTGALLHYGCIAGAILGQSSQKEYQ
                     ALDDYGRALGEAFQIADDLLDVEGDAAALGKKTGQDAALGKTTFVTQLGIEGAKQRVR
                     DLLARADSALSIFGARGDVLRAAARFVAERKN"
     gene            6896..7573
                     /locus_tag="VN968_25735"
     CDS             6896..7573
                     /locus_tag="VN968_25735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HXL32666.1"
                     /translation="MRVARNLLLILLAVLLLPYLLAPFYRVGHPVSTLMAWRWLTGAP
                     VSRQWIDFVAISPLLPRSVVASEDAKFCSHHGIDWNALRDVIDDAEDGEVARGGSTIT
                     QQVAKNLFLWPGRSVVRKALEFPLALWIDLVLPKQRILEIYLNIAELGPSGQFGAQAG
                     AIYAFDHPSLTLSPREAALLAAILPNPVRRSARNPGPGVRRLAGTYMVRAQAAGLQRC
                     WSENRAF"
     gene            7717..7902
                     /gene="rpmF"
                     /locus_tag="VN968_25740"
     CDS             7717..7902
                     /gene="rpmF"
                     /locus_tag="VN968_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HXL32667.1"
                     /translation="MAVPRRKTSPSRRGMRRSADALKKPTYAEDKDSGELRRPHHLDL
                     KTGMYKGRQVLKAKKES"
BASE COUNT         1574 a         2582 c         2681 g         1401 t
ORIGIN      
        1 cttgcgcgac gccgagccac ctcacgaccg gcggcagccc cagcccaagt cccaccagcg
       61 tgatgaaaat gacgccgaaa gagacgaaca ggatcaggtc gcggtatgga aaatcctcgc
      121 cgccgggaag cgccaatggc agcgccagtg ctgcggccag cgagaccgcg ccgcgcacgc
      181 cggtgaaggc gatcacgaat accgtgcgcc aggatggcat cggatcgcgg gcgcgccggc
      241 gcttgttcaa cagccgcggc aggtaggttg cgggaaagac ccaggcgaaa cgcgcgacga
      301 tgacgatgat ggcgacgagc gcggttgcga acaggatatc gtcaagcgga aacgctttcg
      361 atttctcgaa cagcaaccgc atctggaatc cggtcagcag gaacagcagg ccctcgatca
      421 gataaatcac aaggtcccag aaaaagatgc cctgcagccg ggtcgcggac gagatcagga
      481 gcggcccgtt ccagctcata tagagcccgc aggcgacggt cgagatcaca ccggagccgc
      541 cgaaatgctc cgggatccag taggcgatat agggggtgat caacgacagc gtgatttcga
      601 cctgcgggtc gcgcgcgcgg tggcgcatcc tcaggctgag ccagcccacg gcggcgccga
      661 acagcacctc gccggcgacg atgacgacga aggtgcctgc ggccttcggc agcgaaaaca
      721 tgccggtcga gatcgccgcg accgcgaagc gatacaggat cagcgcagtg gcgtcattcg
      781 ccagtccctc gccctcgagg atgaccagga tacggcgcgg catgccgagc ttgcgggcga
      841 tggcgagcgg cgccaccacg tccggcggtg cgacaatggc gccgagcagg aagccgacat
      901 tccacggcag tccaatcaga taatgcgtcg cggcggcgac cgtgaacgcc gtgaaaatca
      961 cgcagccgac cgacagcagg atgatcgggc gcaggttgtg cttgaattcg cgccagctca
     1021 tggcgacgct ggccgaatag atcagcggcg gcaataccac cagcagcacc agttccggcg
     1081 gcagttccag cgacggcatc cccggcacga aggcgagagc gatgccggtc agcagcagca
     1141 ggatcgccgg tgcgacgttg atccgccgcg ccagcagcgc ggcgcccgcc agcagcgcca
     1201 gcagaatcag aaatatctgg aatttggctt ccacgggttc ttcaaatcct tccgcccctc
     1261 aattcgcgcg gaaggcgggc aaggtcaagg cggcggtatt cactcgcgca tatcgtagcg
     1321 ataggatttg gagacgatct gccagccgtc ggcgagtttc atcgccacca gatagtcggt
     1381 gaagtagcgc ggcggcaatt ggcagcgcac cttgatgaag gcggtggatt cgtcggagcg
     1441 gtcgatggtc acgatgaagt cctcgcgcgg cttgccttcc gccttggcgg atggccgttt
     1501 cctgacgcgg tcgagccagt cgggcacggt gaggacttgc agttcaccct tctccagcca
     1561 gcgcaggtcg gcggaggggt ggaagatggc gccgagcttg tcggcgctgc cctcataaag
     1621 gccgtcgaaa tagtgttgga cgacggcctc gacggtggaa cggtcatgac tcatggttgg
     1681 gctccctgct tacgtttgcc aattgctcgt cattccgggt tcgctcgttt cactcgcgcc
     1741 ctcagatgtg caattgcaca tcggggaatg acagcttacc tacatcagaa acacgccttt
     1801 gccgtcgacc tgcctgtcga acagtttgta ggcctcgtcc gcctgatcga tgctccagtg
     1861 gtgggtgaag agtttgtcga cgtcgatgcc gcgctcgacc gaaaatcgcg cgcattccgc
     1921 ctggatgatg ttggagaacg tccatgacgc caccagcgtc aactgccggc gcaacagatg
     1981 cgggctgacg tcgatgtgaa catcgccgcc ctcgccgacg aaacacatgg tgccccacac
     2041 cttggtcgag cggatcgcat tcaatcgcgc atccgggttg ctcgatgtgt ccagcgtcag
     2101 atccgcgtag cgtccgtggg tgagatcctt gatggcggcg accggatcgt tcgagccggg
     2161 attgatcacc tcatcggcgc cgaacgcttt ggcgcgctcc agccgctgct ggctgatgtc
     2221 gagcgcaatc accctggcgc ccatcgcctt ggcgtattgc gtggccgcca atccgaccgg
     2281 gccctggccg aagaccgcca gcgtgtcgtt gcccgacagg ttcatgcggc gcagcgcgct
     2341 gtaggcggtg ccggagccgc aggcgattgc ggcacccgcg ctgaatgaca gttcgtcggg
     2401 cagcggcacc agcgtgttgg cgggcacctt caaatagtgg gcgtggccgc catgcgcgtt
     2461 gttgccgtag accttcaccg gcacctcctg gcagagctgc tgccatcccg agcggcaatg
     2521 gttgcactgg gtgcagccct gataatggtg caccatcacg cgctgaccga tatgcgcctg
     2581 ctttgcatcg acggcggtgc cggccgcgac caccacgccg cagggttcgt gtccggcgat
     2641 cacggggccg tccttcatcg ggatgccggt ggcctgcgtc tggcccttcg ggcggcggta
     2701 ctgatgcagg tcgctgccgc acatgccgga cgccttcatt tcaatgacga cgtcatgagc
     2761 atccggagtg ggatcgggga actgcatcag ttccagtccg cgctcgcccg tgaatacaac
     2821 gccgcgcatc gtgtcctccc ttgcttttgt tggtgtcccg atcttggacg gaaaaacatg
     2881 gttggcaaag ggcgaacctg gagcgatctg gcgcgactaa taacggggag ttccgttgcc
     2941 tgctctgccg ccgcagcaat ggatgcatag acggcacctg cgtcccatgc gtgacgtggc
     3001 tcacatggtc cggcgcaggc ttctgataga tagactgcag tgctataagg ggcttggttc
     3061 cggaagtgtc atcatgaaca cgcatcgcta tttaaagttg cgtaaaattt tccttgcggc
     3121 cgctctgctg ctgatgtgcc agccggcctg ggccgaaaaa cgcgtggcgc tggtgctcgg
     3181 taactccgca tatcaaaacg tggcgcagct tccgaacccg gtcaatgacg gcgcgataat
     3241 cgcggcaaag ctcagggagg ccggcttcga tgttgtcgat tcacgccacg atctgccggc
     3301 ggcggagacg cgtcgcgcgt tgcgcgactt tgccgaccgc tcccgcgacg ccgacatcgc
     3361 cgtggtctat tacgccggcc acggcatgga agtggacggt acgaactacc tgatcccggt
     3421 cgatgcgaag ctcgaacgcg acaccgatgt gtacgacgag gccttttcgc tcgatcgcgt
     3481 gctgctggcg atcgagcccg ccaagcgcct gcggctcgtg attctggatg cctgccgtga
     3541 caatccgttc tccaaaagca tgaaacggac gctggcatcg cgtgcgatcg ggcagggtct
     3601 tgccaagatc gagccgagca gcccgaatat gctgatcgcc tattccgcca aggccggctc
     3661 cacggccgcg gacggcgacg gcaagaacag cccattcacg gttgcactgt ccaagtatct
     3721 tacggagccc gggcttgacg tgcgccgggc gttcggattc gtgcgcgacg acgtgctgaa
     3781 gaacaccagc aacaggcagg agccgtttgt ctatggctcg ctcggcggcg aggatgtgcc
     3841 cctggtgccc gcgcctgcca aggctgccgc accgtccacg cctgcgccca gtccccaggc
     3901 cgaggcgcgc cgggactacg aattggcgct gcaggtcggc aacaaggacg caatgagcgc
     3961 cttcctcgcg caatatccga atggcttcta cgcaaacctg gccaaaatcc agctcggcaa
     4021 gcttgccgcg gaggaagcgc gactggcggc gaccgaaaag gcgcggctgg ccgaacagga
     4081 gcgggcgcgg cttgccgccg agggcacgca aaaggcgcag ttgaaggcgg ccgaggctga
     4141 tgcgaaagcg gcggaacagg cccggatcgc cgccgaaaga gccaaacagg tagcgcagga
     4201 gaaggccgcc gaggccgagc ggaagcgggt cgcgaccgaa acgccagcca ccgacaaggg
     4261 tcccgagatc ataactgctg ccggtaaccc ggcctcggac agggccgcgg ccagctcgcc
     4321 ggcggccgag aagccccaaa ccgagaaagc ggtgaacgtt gccgcgctca gctccggccc
     4381 gccgccggct gagctcacca aaccagtgca aaccgctgat ctcaccaagt cggtgcagac
     4441 cgagctgcgc cgcgtcggct gtctcagcgg agatgccaat ggcgaatgga acgcggcgtc
     4501 gcagcgttcg atggcgctgt tcaaccgcaa tgccaagacg aaattcgacg tcaaggttgc
     4561 aagcgtcgat gcgctggatg cgatcaagct gaagccggcg cgggtctgtc ccctgatttg
     4621 ccaacacggc ttcgaggccg acggcgatca ttgcagcaaa atcgtctgcg gggagggctc
     4681 gttcctcaat gatgccaacg aatgcgaaaa gcggcgcgag aaaacaccga ccgcacggcg
     4741 cgatcgtgac gggcgccccg atcgctccga gcgtctcgca cgccccgagc gtctcgatct
     4801 cccggttcgg ccgcggccgg cgcccgaggt ccgcgtgtcc agaccccagg catcgggcca
     4861 gatcgtttgc gacgcgggcg gttgccggcc ggttaggcgc ggctgtcgca tcgactatca
     4921 gggtggctca cctcgcagtg gcagcggagg caatgaggaa gtctgcaact gagggcggtg
     4981 gggggttgac tttgggctgg aagaccgcac agggaggccc ctacgagggc ggtggcagta
     5041 acatccagat ttgcccctga tccatccgcg actgccgatg cggctggcca ctaaatcgcg
     5101 cttgccgcga tattgaccat caaggcgacc agcgcggtgt tgaaaacaaa ggagatgatg
     5161 ccgtgcgcgg tcgcggtgcg gcggatggtc ttgtcggtaa taccgacgtc ggagacctgc
     5221 gccgtcatac cgatcacgaa cgagaaatac acaaagtccc agtagtcggc atggtcgtgc
     5281 tcatcgccgc tcggaaattg caagccgcca ggtttggcgc cgcggtaata atcgtgggcg
     5341 taatgcaatg cgaaaatggt gtgcacagca gaccaggaca gcgcgatcgt cacggtcgcc
     5401 agcgctagtt ccggtacgct acggtgcgat gcgccgagtt cgaacacgat cgcgccgatg
     5461 ctggcgaacg cgccgagcgc agtgaccagc aagatcagaa aacggccatc gtcctgcagc
     5521 accgcgttac ggcggatatg gctgtgatcg cagcgaaaca tcatgacata ggcgagcacg
     5581 agatagacag taacgaagat atcccaactg atcaggaggc gagtgaccag ccgcagcgag
     5641 cccggcagca ggaagaacgc cacgataccg attgcgaccg aagcaaacag gcgcggccgc
     5701 gcgtagacca cgcgaaccgg cttggacatc ttccggaacc ggacaagaag aggatcttcg
     5761 gtttccttgc ccgccatcgg cgccctcaat tcttccgttc ggcgacgaac cgcgcggcgg
     5821 cgcgcagcac gtcacccctc gcgccgaaga tcgacaacgc ggaatcggcg cgcgccagca
     5881 gatcgcgcac gcgctgcttg gcgccctcga tcccgagctg ggtgacgaaa gtggtcttgc
     5941 ctagtgcggc gtcctgtccg gtctttttgc cgagcgccgc cgcatcgccc tcgacatcga
     6001 gcagatcgtc ggcgatctgg aaggcttcgc cgagtgcgcg gccgtaatcg tcgagcgcct
     6061 gatattcctt ctgcgaggac tggccgagga tcgcgccggc gatgcagcca tagtgcagca
     6121 acgcaccggt cttcatctgc tgcaggcggg cgacgtcgac cggttcgcgg tcgccgaaac
     6181 ggccctcgcc ggcgaggtcg aggatctggc cgccgaccat gccgccgacc cccgacgccc
     6241 gcgccagcgc gcgcgtgagc agcaggcgta cgctcgcctc gcgatggatc tcgtcgcggc
     6301 tgatgatgtc gaaggcgagg gtcagcaggg cgtcgccggc gaggatcgcg gtggcatcgt
     6361 cataggcctt gtgaagggtg ggccggcccc ggcgcagatc gctgttgtcc atcgccggta
     6421 gatcgtcatg gatcagcgaa tagcagtgga tgcactccag cgcggcgccg gccagcagag
     6481 cggccttccg cgcgacaccg aacacggccg agctctcgac caccaggaac ggccgtagcc
     6541 gcttgccgcc gccgaggctc gagtaacgca tggcctcgat cagccgtttc ggacgcgcga
     6601 tctcgtcggg caaaagcgta tcggataaca gctccgcgag cagggtctcg gtatcctccg
     6661 cggtctggtc cagtcgtttc gcaaagtctg atggtgcagt gccagtcgtc atcaaaacag
     6721 ctccagatcg agttgcgccg ggacaatcgg ttatggcatc ggcttcgtca attcctccgc
     6781 cggagttcaa tgcgccttaa aagtgctgga aaaaccgggc gctatcgtgg agatgtcatt
     6841 ggatgccggt ccttgggccg gtcgcgcccg ctgccttgaa ccagaaacat caaatttgcg
     6901 cgttgcccgg aatttattgc tgattttgct ggccgtgctg ttgctgccat atctgctggc
     6961 ccccttctat cgcgtcggcc atccggtatc gacgctgatg gcctggcgct ggctgaccgg
     7021 cgcgccggtg tcgcggcaat ggatcgattt cgttgcgatc tcgcccttgc tgccgcgctc
     7081 ggtggtcgcg tccgaggacg cgaaattctg cagccatcac ggtatcgact ggaacgcctt
     7141 gcgcgacgtg attgacgacg cggaggacgg cgaagtggcg cgcggcggct cgaccattac
     7201 ccagcaggtc gcgaaaaacc tgttcctgtg gccgggccgc agcgtggtcc gcaaggcact
     7261 ggagtttccg ctggcgctgt ggatcgatct ggtgctgccg aagcagcgga tcctggaaat
     7321 ctatctcaac attgccgagc tcggaccatc gggtcagttc ggcgcgcagg ccggcgccat
     7381 ctatgccttc gaccatccgt ccttgacgct gtcgccgcgg gaggcggcgc tgctggcggc
     7441 gatcctgccc aatccggtca ggcgcagcgc ccgcaatccc ggccccgggg tgcgccgtct
     7501 ggccgggacc tatatggtcc gggcgcaggc cgcgggattg cagcgatgct ggagcgaaaa
     7561 tcgcgctttt tgagccggtt gggtgtattt tgaacctagc cttggctccc cccatcctct
     7621 ataagcgcgg ccttatcggc atttcagctc gcgccaaaac gcttcagccg tccgcttggg
     7681 acgatgcctc cgacaacacc cctagaggac accgacatgg ccgttccccg aagaaaaaca
     7741 tcgccctcgc ggcgtggcat gcgccgctcg gcggacgcat tgaagaagcc gacctatgcc
     7801 gaggacaagg attccggcga attgcgccgt ccgcatcacc tcgatttgaa gaccggcatg
     7861 tataagggcc ggcaggttct caaggccaag aaggaatcct gaccaaggcc gccgaggcct
     7921 gggcaggctg tgagccacgt cgctcgacgt caggacccgg atcggccagg cacggctcgc
     7981 gcgcggcatc cgggccacct cgacaagcga aggcgtggcg cgccgccctg gggtccgcga
     8041 tgcccggtgc ccgagtacgg gcgtcgcggc gtaactcaga gtcggaaccg ctataaccgg
     8101 caacgcctgc ctgaattcgg ccaacgagtg cggtaagacg gtttttctga ggccagcccc
     8161 cacagcgatt cgctgcggaa tgctttggtt ctcttgcttg aaagaaggcc tcgccgatgt
     8221 cgatgatcgg ttttccgc
//
LOCUS       DATNOI010000635         2215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1849985,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000635 DATNOI010000000
VERSION     DATNOI010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849985"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..802
                     /locus_tag="VN968_25745"
     CDS             191..802
                     /locus_tag="VN968_25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32668.1"
                     /translation="MLRRILICMIAAATALTVADAASARQRRHIDATPFSHAPCSVLD
                     GQPCTPSFCSVFNHGPCIPEIDYPINENLQVTVQTVPSRDDAAKYQKPDHDLDTIGDL
                     FAALRSCWTPPPADSAREGMQMSVLFSFKRSGEMIAAPRMTYATAGVSADTRATYLKA
                     INASLDACEPLKFSDGLGGAIAGRPIAIRYVDNRELGKQSGTP"
     gene            1063..1647
                     /locus_tag="VN968_25750"
     CDS             1063..1647
                     /locus_tag="VN968_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HXL32669.1"
                     /translation="MGLLVMILGLVLFLGVHTLTTQRELRARLIVSSGEGGYKIGYAL
                     VSVAGLALIVWGFANYRATGWIDVWYPPTALKHITVALMLPAVILVVAAYIRGRIYTT
                     VKHPMLTGVKLWAAAHLLANGDFGSIILFGSFLGWAVYDRISLKHRADAGAPPIPVGG
                     PTNDLIAVAVGIVAYLALAFAFHPVVIGVPVFGV"
     gene            1649..>2215
                     /locus_tag="VN968_25755"
     CDS             1649..>2215
                     /locus_tag="VN968_25755"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HXL32670.1"
                     /translation="MSIQSAIKRKTAPDIRARKNGEPIVMLTSYHAHTAALVDRHCDV
                     ILVGDSLGNVMHGFETTVPVTLDMMILQERAVMRGSQHALVVVDMPFGSYEASKEQAF
                     HSAARVLKETHCGAVKLEGGARMAETVAFLVQRGIPVMGHIGLTPQSINTLGSFRAQG
                     REENDWKPIENDANAIAQAGAFSVVIEAV"
BASE COUNT          394 a          690 c          693 g          438 t
ORIGIN      
        1 atccagcgca aggaatactg ggtcgcccgg tcaggtgtat gcccggggac atgggtcggg
       61 cgcgatgctc gtcgtgggac tctcggtgaa aaacgcgcgg atgtgaacga aaccaggctt
      121 gatagtacct gccgtgggca tcattttccc tttaacatcc ttagtcctgt tgcattcgaa
      181 ggctggctcc atgctccggc gcatccttat ctgcatgatc gctgccgcta cggcattgac
      241 ggttgctgac gcggccagcg cccggcagcg ccgtcacatc gacgccacgc cgttttcaca
      301 cgcgccctgc agcgtgctgg atggacagcc gtgcacgccg tcgttctgca gcgtgtttaa
      361 tcacgggccg tgcattcccg agatcgatta tcccattaac gaaaacctgc aggtgacggt
      421 ccaaaccgtg ccttcgcgcg acgacgccgc aaaataccag aagcccgacc acgatctcga
      481 caccatcggc gacctgtttg cggcgttgcg ctcatgctgg accccgccac cggctgactc
      541 cgcgcgcgag ggcatgcaga tgtcggtgct cttcagtttc aagcgctccg gcgagatgat
      601 tgcggccccg cgcatgacgt acgccaccgc aggggtttca gcggataccc gcgccaccta
      661 tctgaaggcg atcaacgcgt cgcttgacgc ctgtgagccg ctgaaattca gcgacggact
      721 gggcggcgca atcgccgggc gcccgatcgc gatccgctat gtcgacaatc gcgagttggg
      781 caagcagtcg ggaacgccgt gagggcgtga gtgtgccccc gtcattccgg ggcgcgccga
      841 aggcgcgaac ccggaatccc gaagttggct tgcaggtttc ataagatcga gattccccga
      901 tgtgcaattg cacatctgag gtctggtcct tcggaccatc ccggaatgac tcctgaaacg
      961 gcccggagcc acttcgccgc gccgagcatt taccgcattg cggcttggac ccctaaagtg
     1021 atacgcgaag ctagcaaaac cggccctgcc aaggaggaca tcatgggact gctggtgatg
     1081 atcctgggcc tcgtgctgtt cctcggcgtc catacgctca ccacccagcg cgagctgcgc
     1141 gcgcgcctaa tcgtgtcatc gggcgagggc ggctacaaga tcggctatgc gcttgtctca
     1201 gtcgccggcc tcgcgctgat cgtctggggg tttgccaatt atcgcgctac ggggtggatc
     1261 gacgtctggt atccgccgac ggcgctcaag cacatcacgg tcgcgctgat gctgcctgcg
     1321 gtcatcctgg tggtggcagc ctacatccgt ggccgtatct ataccacggt gaaacatccg
     1381 atgctgacag gcgtcaagct gtgggcggca gcgcatctgc tcgccaacgg cgatttcggc
     1441 tcgatcatcc tgttcggctc gttcctgggc tgggcggtat acgaccggat ctcgctcaaa
     1501 catcgcgccg acgccggcgc cccgccgatc ccggtcggcg ggcccaccaa cgacctgatt
     1561 gcggtggcgg tcgggatcgt cgcctatctg gcgctggctt tcgcgtttca tcccgtcgtg
     1621 atcggcgtcc ccgtcttcgg agtttagaat gtccatccaa tcggccatca agcgcaagac
     1681 cgcaccggac attcgcgccc gcaagaacgg cgagccgatc gtgatgctga cgtcgtatca
     1741 cgcgcatacc gcggcattgg tcgatcgcca ttgcgacgtc attctggtcg gcgactccct
     1801 gggcaatgtg atgcatggtt tcgagaccac ggtgccggtg acgctcgaca tgatgattct
     1861 gcaggaacgc gccgtgatgc gcggctcgca gcatgcgctg gtcgtggtgg acatgccgtt
     1921 cggttcctat gaagcctcaa aggagcaggc gtttcattcc gcggcgagag tattgaagga
     1981 aactcattgc ggcgcggtga agctcgaagg cggcgcgcgg atggcggaga ccgtcgcgtt
     2041 tcttgtccag cgcggcattc ccgtgatggg ccacatcgga ttgacgccgc agtcgatcaa
     2101 tacgctcggc tcgtttcgcg cccagggccg cgaggagaat gactggaagc cgatcgagaa
     2161 cgacgccaat gcgatcgcgc aagccggcgc attttcggtg gtgatcgagg cggtg
//
LOCUS       DATNOI010000636         2244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2388565,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000636 DATNOI010000000
VERSION     DATNOI010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2388565"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..>2244)
                     /locus_tag="VN968_25760"
     CDS             complement(91..>2244)
                     /locus_tag="VN968_25760"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HXL32671.1"
                     /translation="WMPTLAGDVQMVLADGKVLFQGQEVAFVVATDRYAADDGINKVV
                     VEYEPLPVLIDPFKALDPDAPVLREDLAGKTSGAHGPRRHHNHIFEWTVGDKDLTDAA
                     FRKAEVTIKEMISYHRTHPSPLETCQCVCSFDKIKGELTIWGTFQAPHVIRTVVALIA
                     KIPEQKIHVISPDIGGGFGNKVGAYPGYICAAVASIVTGKPVKWVEDRIENLTATSFA
                     RDYHMTTEIASTKDGKVTGLRVHVLADHGAFDACADPSKWPAGFFNIVTGSYDFPVAH
                     LSVDGIYTNKAPGGVAYRCSFRVTEAAYCIERAMDILAQKLGMDPAELRLKNFVRPEQ
                     FPYHSALGWEYDSGDYATAMKKAMESVKYAELRKEQTEKRAAFKRGETREIMGVGVSF
                     FTEIVGAGPSKNCDILGIAMFDSCEIRLHPTGAGIARVGTKSQGQGHETTWAQIIATE
                     IGIPADDIMVEEGNTDTAPYGLGTYGSRSTPVAGAAIAMAARKIKAKAQMIAAYKLEV
                     HEDDLEWDIDGFRVKGLPEKTMSMKDICWAAYNSVPPGMEPGLEAVSYYDPPNMTYPF
                     GAYICVMNIDVDTGVYKIRRFYALDDCGTRINPMIIEGQVHGGLTEAFAIAMGQEIRY
                     DPEGNVVTGSFMDFFMPTAVETPHWETDFTVTPSPHHPIGAKGVGESPNVGGVPAFSN
                     AVNDAFSFLGSTHIQMPHDYWRNWRTAKELGVFAA"
BASE COUNT          393 a          708 c          720 g          423 t
ORIGIN      
        1 actctctgcc agaggagcaa gcggatggac ccccaccccc gacccctccc cgccacgcgc
       61 aagagcgcgt ggagggaggg gagaagatta tcacgccgca aacactccca attcctttgc
      121 cgtccgccag ttgcgccagt agtcgtgcgg catctggata tgggttgagc cgaggaacga
      181 gaaggcgtcg ttgacggcgt tggaaaacgc cggcacgccg ccgacattcg ggctctcgcc
      241 gacgcccttg gcgccgatcg ggtgatgcgg cgacggcgtc acggtgaagt cggtctccca
      301 atgcggcgtc tcgaccgcgg taggcatgaa gaaatccatg aacgaaccgg tgacgacatt
      361 gccctcgggg tcgtagcgga tctcctggcc catggcgatc gcaaaggctt cggtcagccc
      421 gccatgcacc tgaccctcga tgatcatcgg gttgatgcgg gtgccgcaat cgtcgagcgc
      481 atagaagcgc ctgatcttgt agaccccggt atcgacgtcg atgttcatca cacagatata
      541 ggcaccgaac ggataggtca tattgggcgg atcgtaatag ctcaccgcct ccaggcccgg
      601 ctccatgccc ggcggtaccg agttgtaggc ggcccagcag atatccttca tcgacatcgt
      661 cttctccggc agtcccttga cgcggaagcc gtcgatatcc cattcgaggt cgtcctcgtg
      721 gacttcgagc ttgtaggcgg cgatcatctg cgccttcgcc ttgatcttgc gcgcggccat
      781 cgcgatcgcg gcgcccgcga ccggcgtcga acgcgagccg taggtgccaa ggccgtaggg
      841 cgcggtatcg gtattgccct cctcgaccat gatatcgtcg gcgggaatgc cgatctcggt
      901 ggcgatgatc tgggcccagg tggtctcgtg gccctggccc tggctcttgg tgccgacccg
      961 ggcgatgccc gcgccggtcg gatgcaggcg gatttcgcag gaatcgaaca tcgcaatgcc
     1021 caggatgtcg caattcttcg acggtccggc gccgacgatc tcggtgaaga acgacacgcc
     1081 gacgcccatg atctcgcggg tctcgccgcg cttgaacgcc gcgcgcttct ccgtctgctc
     1141 tttccgcagt tcggcgtatt tgacgctctc catcgccttc ttcatggcgg tggcatagtc
     1201 gccggaatcg tattcccacc cgagcgccga gtgataggga aactgctccg gcctgacaaa
     1261 attcttcaac cgcaactcgg ccgggtccat gccgagcttc tgggcgagga tgtccatggc
     1321 gcgctcgatg caataggcgg cctccgtcac ccggaaggag cagcgatagg cgacgccgcc
     1381 cggcgccttg ttggtgtaga tgccgtcgac ggagagatgc gcgaccggga aatcgtagga
     1441 gccggtgacg atgttgaaga agccggccgg ccacttcgag ggatcggcgc aggcgtcgaa
     1501 cgcgccgtga tcggccagca catggacgcg aaggcccgtg accttgccgt ctttggtcga
     1561 cgcgatctcg gtggtcatgt ggtaatcgcg cgcgaacgaa gtcgcggtca ggttctcgat
     1621 gcggtcctcg acccatttta ccggcttgcc ggtgacgatc gaggcgaccg cggcacagat
     1681 gtagccggga taggcgccga ccttgttgcc gaaaccgccg ccgatatcgg gcgagatcac
     1741 atggatcttt tgttccggaa ttttcgcgat cagcgccacc acggtgcgga tcacatgcgg
     1801 cgcctgaaaa gtgccccaga tcgtcagctc gcccttgatc ttgtcgaacg aacagacgca
     1861 ctggcaggtc tccagaggcg acggatgggt gcggtgatag gagatcatct ccttgatggt
     1921 gacctcggcc ttgcggaagg cggcgtcggt caggtccttg tcgccgacgg tccattcgaa
     1981 gatatgattg tggtgcctgc gcggcccgtg cgcgcctgag gtcttgccgg cgagatcctc
     2041 gcgcagcacc ggcgcatccg ggtccagcgc cttgaacggg tcgatcagca ccggcagcgg
     2101 ctcgtattcg accacgacct tgttgatgcc gtcgtcggcg gcatagcggt cggtggcgac
     2161 cacgaaggcg acctcctggc cctggaacag caccttgccg tcggccagca ccatctgcac
     2221 gtcgccggcc agcgtcggca tcca
//
LOCUS       DATNOI010000637         2382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_963321,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000637 DATNOI010000000
VERSION     DATNOI010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963321"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VN968_25765"
     CDS             complement(<1..471)
                     /locus_tag="VN968_25765"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HXL32672.1"
                     /translation="MQETGLRNSAYGADRFGFKDLRAIHWNLTAPSLYEHAIAAREAS
                     IVEGGALCAETGVHTGRSPKDKHTVVDALTENAVWWDGNRKITPENFQRLYDDFIAHA
                     RGKELYAQDLYGGADPNYRIKTRVFTELAWHSLFIRQLLIRPVAPELEDFVPELT"
     gene            complement(621..1640)
                     /locus_tag="VN968_25770"
     CDS             complement(621..1640)
                     /locus_tag="VN968_25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HXL32673.1"
                     /translation="MRRLGIVLAVSLAAGSAWAQGKEPIALRDMGSFHVGGRVIEITG
                     QPIKEIVFTPGGVPAKMDPNGKYQVEQMYVQYFLPQNRRGRLPLLMWHGGGLTGVTYE
                     TTPDGRAGWLSYFIRKGWDTYISDAVERGRAGWTDTFKGDALSLPLGDPWERFRIGPP
                     GSWNDDKAKRTTYPGAQFPIEAYAQFMKQGVPRWLTTDDQIVAAYIELVDKVCPCVVM
                     VHSQSGTFGYRVLEARPDKVKALVAVEPTLGGDRNKVESIKTTPILVVYGDNAKDHPR
                     WSKIRQGGVDYAAVLRSAGGSIDVVDLPDVGIKGNSHMMMMDKNNAEIADLIQKWLLD
                     KGLAQ"
     gene            complement(1694..>2382)
                     /locus_tag="VN968_25775"
     CDS             complement(1694..>2382)
                     /locus_tag="VN968_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HXL32674.1"
                     /translation="FVRKGWDVYVSDAVERGRAGFASPDVWPSEPIFLNYADPFERFR
                     IGGGEGSWNADSAKRKVLPGSQFPVEAYDNYMKQAVPRWLSTDKAVVDAYVALVDKVC
                     PCVLLLHSQGGAFGFKVAEQRSDKIKAIVAVESASAGDVRKAAALKNTPTLMLFGDYV
                     DQYPRWATYKKIDLEYAAAMRAGGGTVDVVNLPDIGIKGNSHMLMQDKNNAEIADVIQ
                     AWMVGKGLVE"
BASE COUNT          407 a          779 c          737 g          459 t
ORIGIN      
        1 ggtgagttcc ggaacaaaat cttcgagctc gggggccact gggcgaatca agagctgacg
       61 aatgaaaagc gagtgccacg ccagctcggt gaagacccgc gtttttatcc ggtagttcgg
      121 atcggcgccg ccatagaggt cttgggcgta aagctccttg ccgcgcgcat gcgcgatgaa
      181 atcgtcatag agccgctgga agttttccgg ggtgatcttg cggttgccgt cccaccagac
      241 cgcgttttcg gtcaatgcat cgacgaccgt atgcttgtcc ttgggcgacc ggccggtgtg
      301 gacgccggtc tccgcgcaga gcgcgccgcc ctccacgatc gaggcttccc tggccgcgat
      361 cgcgtgctcg tagagcgagg gcgcggtgag attccagtgg atcgcgcgga ggtccttgaa
      421 gccaaatcga tcggcgccgt aggcgctgtt ccgcaaaccc gtttcctgca cgaaagacct
      481 ccacgacgca gccggcggcc gccggatcga ctccccgtcg ccatatctgg aaggctgaat
      541 gatagcgccc gccgctcgat cccgcgacct ataattggcc tcgcgcggct gtcaatgctg
      601 cgccgcagca gcacacgcgt tcattgcgcc aatcctttgt cgagcaacca tttttggatg
      661 aggtcggcga tctcggcgtt gttcttgtcc atcatcatca tgtgagaatt gcccttgatg
      721 cccacgtccg gcaggtcgac cacgtcgatg ctgccgcccg cggatctaag aacggcggcg
      781 taatcgacgc cgccctggcg gatcttcgac caccgcgggt ggtcttttgc attgtcgccg
      841 tagacgacga ggatcggcgt ggtcttgatc gattcgacct tgttgcgatc gccgcccagg
      901 gtcggctcca cggcgaccag cgccttgacc ttgtccggac gcgcctccaa caccctgtag
      961 ccgaacgtcc ccgactggct gtgcaccatg accacgcagg ggcagacctt gtcgacgagt
     1021 tcgatataag cggcgacgat ctgatcgtcg gtggtgagcc agcgcggcac gccctgcttc
     1081 atgaactgcg cataggcctc gatcgggaac tgggcgccgg gataggtcgt gcgcttggcc
     1141 ttgtcgtcgt tccacgaacc gggcgggccg atgcggaagc gctcccacgg atcgcccagc
     1201 ggcaaggaca gcgcgtcgcc cttgaaggtg tcggtccaac cggcgcggcc gcgctccacg
     1261 gcgtcggaga tataggtgtc ccagcccttg cggatgaaat agctgagcca gcctgcgcgg
     1321 ccatccggcg tggtttcgta ggtcacgccg gtgagcccgc cgccgtgcca catcaacagc
     1381 ggcagcctgc ccctgcggtt ttgcggcagg aagtactgca cgtacatctg ctcgacttga
     1441 tacttgccgt tggggtccat cttcgcgggc acaccgccgg gtgtgaacac gatctccttg
     1501 atcggctggc cggtgatctc gatgacccgc ccgccgacgt ggaacgaacc catgtcgcgt
     1561 aaagcaatgg gttccttgcc ctgcgcccag gccgagcctg ctgcgaggct gactgccaaa
     1621 acgatgccca agcggcgcat ggctccctcc cttctctgtt cgaaatcgga actcttgcga
     1681 gggtcgaacg cggttactcg accaagcctt tcccgaccat ccatgcctgg atgacatcgg
     1741 cgatctcggc attgttcttg tcctgcatca gcatatgcga atttcccttg atgccgatgt
     1801 cgggcaggtt gaccacgtcg accgttccgc cgccggcgcg catcgcggcg gcatattcga
     1861 gatcgatttt cttgtaggtc gcccagcgcg gatactggtc gacatagtcg ccgaacagca
     1921 tcagcgtggg cgtgttcttc agcgccgccg ccttgcggac gtcgcctgcg ctcgccgatt
     1981 cgaccgcgac gatcgccttg atcttgtccg aacgctgctc cgcgaccttg aagccgaacg
     2041 cgccgccctg gctgtgcagc agcagcacgc aggggcagac cttatccacc agcgcgacgt
     2101 aggcgtcgac gaccgccttg tcggtggaga gccagcgcgg caccgcctgc ttcatgtagt
     2161 tgtcgtaggc ctcgaccggg aactggcttc cgggcagtac cttgcgcttg gccgaatcag
     2221 cgttccacga gccctcgccg ccgccgatgc ggaagcgctc gaacgggtcg gcgtagttca
     2281 aaaaaatcgg ctcggacggc cacacgtccg gactcgcaaa gcccgcgcgg ccgcgctcga
     2341 ccgcgtcgga gacgtagaca tcccagccct tgcggacgaa ca
//
LOCUS       DATNOI010000638         3582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_981960,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000638 DATNOI010000000
VERSION     DATNOI010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981960"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VN968_25780"
     CDS             complement(<1..272)
                     /locus_tag="VN968_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL32675.1"
                     /translation="MSRVVWILAVLLSHWRRHPMQLATLLIGLISATALWSGVQALNQ
                     QARTSYDRAAATFGGARTAMLVSRNSATFPQQLFVDLRRAGWPVSPV"
     gene            complement(269..922)
                     /locus_tag="VN968_25785"
     CDS             complement(269..922)
                     /locus_tag="VN968_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32676.1"
                     /translation="MLSVRKLTKTYRAAGEQIVVLRGVNLSVAAGERVALSGESGSGK
                     STLLHLIAGLDRPDGGEIRLADTSVSDLTDAGRAALRRDRLGLVFQQFNLIPSLNVDD
                     NLAFQSRIAGRHDPAWHGELVERLGLGGLLNRYPEQLSGGQQQRVAIGRALAAKPLLL
                     LADEPTGNLDEATADDVLALARDLVKRSGCGFLMVTHSARLAATLDRQINLHAGLIA"
     gene            complement(963..1730)
                     /locus_tag="VN968_25790"
     CDS             complement(963..1730)
                     /locus_tag="VN968_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXL32677.1"
                     /translation="MVRPIFCRTSAIAALAVLSMLSGWSLAAAHDANAPDYEAIVASP
                     DRSDGDRQTDQRRQPAKMLAFIGVRSGMKVLDMEANAGYSTELLARSVGPTGVVYAQD
                     PAAIIERLVKDKFDIRAQKPAMKNVVHVVRDFDDPIPPEGSGLDLITFFFAYHDVTYM
                     PVDRAEMNRKMFAALKPGGFLVIADHSARPGDGTSVGKTFHRIEESVLRQEIEAAGFE
                     LVAEADFLRHPEDPRDVVVFRPVVPVDEFVLKYQKPL"
     gene            1981..3249
                     /locus_tag="VN968_25795"
     CDS             1981..3249
                     /locus_tag="VN968_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL32678.1"
                     /translation="MTTQPTPKGAWKITFLLFLFMLVNFADKIVVGLAGVPIMTELKL
                     DPEEFGLLGSSFFFLYSIAAIVVGFIVNRIATRWVLLALALVWALAQFPMVGTVGFTT
                     LLVCRVILGAGEGPAFSVAAHSIYKWFPDEKRTLPTAILSQGSAFGVILAVPALNWVI
                     VNHSWHYAFGALGVVGLMWVVAWLILGKEGPLVHTVAMAAAEPRIPYFQLLTSRTFVG
                     CCAATFGAYWALSLGLTWFTPFIVNGLGFSQKDAGWISILPWVFGAVVVLLTGWISQV
                     LMARGVTTRGARGVLGAAPLIVGGSILVALPHVDGAGLQIAVLVAGPGLCGSIYVVCP
                     PMLAEFTPVSQRGAVISIYGAIYTLAGILAPSVMGSVVQHAATPLEGYMTGFTINAVI
                     MIVSGLLGLLLLWPNTERARLIGVTQPKFA"
BASE COUNT          618 a         1124 c         1125 g          715 t
ORIGIN      
        1 accggcgata ccggccagcc ggcgcggcgc aggtcgacaa agagttgctg cggaaaagtc
       61 gcactgttac ggctgaccag catagccgtt ctcgctccgc cgaaagttgc ggccgcgcgg
      121 tcgtaactgg tgcgcgcctg ctggttcaac gcctgcacgc cgctccacag cgcagttgcg
      181 gaaatcagcc cgatcagcag tgtcgcgagc tgcatcggat gccgccgcca atggctcaat
      241 agaaccgcga gaatccagac gacgcggctc atgcgatcaa ccctgcatgg agattgatct
      301 ggcggtcgag cgtggcggcg aggcgcgcgc tgtgggtcac catcaaaaag ccgcagccgc
      361 ttcgcttaac cagatcgcgc gcgagcgcga gaacgtcatc tgccgtcgcc tcatcgaggt
      421 tgccggtggg ctcgtcggcg agaagaagca gcggttttgc cgctagcgcc cggccgatcg
      481 caacccgctg ctgctggccg ccggagagct gttcgggata ccggttcaac aaaccgccga
      541 gccccagccg ctccaccagt tcgccatgcc aggccgggtc gtggcgtccg gcaatgcgcg
      601 actggaaggc gaggttatca tcgacattca ggctcggaat caggttgaac tgttgaaaca
      661 ccaggcccaa ccggtcgcgc cgtaatgccg cgcgacccgc atcggtgaga tccgaaaccg
      721 aggtgtcggc caacctgatc tcgccgccgt cagggcggtc gagacctgcg atcagatgca
      781 acagcgtgct cttgccgctg ccagattctc ccgacagcgc gacgcgctcg cctgccgcga
      841 cactcaggtt gacgccgcgc agcaccacga tttgctcacc ggcagcgcga taggtcttgg
      901 tcagtttgcg aacgctcagc acgattgggt tgccaattgc catgcgaagc gtatgcgatc
      961 cctcacaacg gcttctgata tttcagcacg aactcgtcga ccggcaccac aggacggaac
     1021 acgacgacgt cacgcggatc ttcggggtga cgcaggaaat ccgcttcagc cacgagctcg
     1081 aagccggccg cctcgatttc ctggcgcagc acgctctcct cgatgcgatg gaacgtcttg
     1141 ccaacgctcg tgccatcgcc gggacgcgcg gaatggtcgg cgatgacaag aaacccgccc
     1201 ggtttcaacg cggcgaacat cttccggttc atctcggcac gatcgaccgg catataggtc
     1261 acgtcgtggt aggcaaagaa aaacgtgatc agatcgagcc cgctgccctc gggcggtatc
     1321 ggatcgtcga agtccctgac gacatggacc acatttttca tggcaggctt ttgcgcgcgg
     1381 atatcgaatt tgtccttcac caggcgctcg attatcgcgg ccggatcctg cgcgtagacc
     1441 acgccggtcg ggccgacgct gcgcgccagg agctccgtgc tgtagccggc gttggcctcc
     1501 atatccagca ccttcattcc tgatctgacg ccgatgaaag ccagcatttt ggcgggctgg
     1561 cggcgctgat cggtttgccg gtcgccatcg ctgcgatccg gcgaagcgac aatggcttca
     1621 tagtcggggg cgttggcgtc gtgcgccgcc gctaggctcc atcccgaaag catcgaaagc
     1681 acggcaagag cggcaatggc gctggtgcgg caaaatatcg gtctgaccat gaagacccct
     1741 tatcatttgc ctgcaacata gcaaggctgt gccgggagat cacgatctca atcgggtcgg
     1801 cgacgagatc gtgattgggt tgcggaatgc ttctcaagcg gcgtcagtcg gcattcgcag
     1861 gcgatcctga gttgcggcgc atgcaggccg gacaaaatgt ccaattgcat tgccgctgac
     1921 cccgcgggta gcttcggatg aggccgatca acggggccgg aaacgaaaat cggggcaacc
     1981 atgaccaccc aaccgacgcc aaagggcgcc tggaaaatca cgtttctcct gtttctcttc
     2041 atgctggtga atttcgcgga caagatcgtg gtcggcctcg ccggcgtgcc gatcatgacc
     2101 gagttgaaac tcgatcctga agagttcggc ctgctcgggt cgtcgttctt cttcctgtac
     2161 tcgatcgcgg ccatcgtggt tgggtttatc gtcaatcgaa tcgccacgcg ctgggttctg
     2221 cttgccctgg cgctggtctg ggcgctggcg cagtttccga tggtcggcac cgtcggattc
     2281 acgacgctgt tggtctgccg cgtgatcctc ggcgccggcg aaggcccggc gttttcggtg
     2341 gcggcgcatt cgatctacaa atggtttcct gacgagaagc gaacgcttcc aaccgccatc
     2401 ctgtcgcagg gatcggcgtt cggcgtgatc ctcgcggtac cggcgctgaa ctgggtgatc
     2461 gtcaaccata gctggcacta tgctttcggc gcgctcggcg tcgtcggcct gatgtgggtc
     2521 gtggcgtggc tgatcctggg caaggaaggg ccgctggttc acaccgtcgc gatggcggcg
     2581 gcggagccgc gcattcccta tttccagctt ctgacctcgc gaacctttgt cggctgctgc
     2641 gccgctacct tcggcgccta ctgggcactc tcgcttggcc tcacctggtt cacgcccttc
     2701 atcgtcaatg gcttgggctt ctcgcaaaag gacgcgggct ggatttcgat cctgccgtgg
     2761 gtgttcggtg ccgtcgtcgt gctgttaacg ggatggattt cgcaggtgtt gatggcgcgt
     2821 ggcgttacca cccgcggcgc ccgaggtgtg ctgggcgcgg ctcccttgat cgtcggcgga
     2881 tcgatcctgg tcgcgctccc gcatgtcgat ggcgcggggc tccagatcgc ggttcttgtg
     2941 gccggacccg ggctttgtgg gtcgatctat gttgtctgcc cgccgatgct tgcggaattc
     3001 acgccggtgt cgcagcgcgg cgccgtgatc tcgatctacg gcgcgatcta cacccttgcc
     3061 ggcattttgg ccccatcggt gatgggcagc gtggtccagc acgcggcgac gccgctcgag
     3121 ggctacatga ccggcttcac catcaacgcc gtcattatga tcgtgtctgg attgctcggc
     3181 ctgctgctgc tctggcccaa caccgagcgc gcgcgtttga taggggtgac gcaaccgaag
     3241 tttgcgtgag agcgccaata ttaccgcgcc gtccccgcga acgcggggac ccataaccac
     3301 aagcggtgtt tgtttatcga aggcgtctac cccatcctaa ccgataggtc acggcgtttg
     3361 ggtccctgct ttcgcaggga cgacggtgag tttgttgtag cgtttgcgtc ctaacctact
     3421 gcaaactgcc ctgcgcgccg cgcaccagcc ggcccggacg cgcgccggtg gcggtcccgc
     3481 cacgctgcgt caccacgccc gagacgatgg tagcgtcata gccatcgacc tgttgcagca
     3541 aacggcggcc gccgaccggc agatcgtaat gcattttcgg tg
//
LOCUS       DATNOI010000639         2069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_458934,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000639 DATNOI010000000
VERSION     DATNOI010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458934"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VN968_25800"
     CDS             <1..858
                     /locus_tag="VN968_25800"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32679.1"
                     /translation="DIPNTWDAFLDFFMPVQDKLRAQGMRNIYAYGYQLTANGVDPGI
                     LFQAFLMAYGGKGLVTPDGKLHTEDPQIREAAIRAIEKLTTAFKKGYVPPSVINWNDA
                     DDNNAFHAKLVVMDFDGFSTEVAVYKNKALYDDIVTRGMPLGNDGKELASPMGVFGAL
                     IPKGAKNVTVAKDFLKYAVQPNVLNEYLKGGLGRWVIPMREIAKADPFWFREDKNRTA
                     FAEETLTHPTVPLYQAFNPAYAQVDSEHVFSIAMLDVMNNGMILEQAVGKAFKRAEEI
                     FAKYPITEA"
     gene            1854..>2069
                     /locus_tag="VN968_25805"
     CDS             1854..>2069
                     /locus_tag="VN968_25805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32680.1"
                     /translation="MRGSGRLCGAGRLIARSAAVVLILGFGSVAVLGDEPAKPMTIVV
                     FGDSQAQGLADGLSRVLVEDARYRVLNR"
BASE COUNT          398 a          639 c          647 g          385 t
ORIGIN      
        1 gacattccga atacctggga tgcctttctc gactttttca tgccggtgca ggacaagttg
       61 cgggcacagg gcatgcgcaa catctacgcc tacggctacc agctgaccgc caacggggtc
      121 gaccccggca tcctctttca agcgttcctg atggcctatg gcggcaaggg cctggtcacg
      181 cccgacggta aattgcacac cgaggacccg cagattcgcg aggcggcgat tagggcgatc
      241 gagaaactga ccaccgcctt taaaaaaggc tatgtgccgc cgagcgtgat caactggaac
      301 gacgccgacg acaacaacgc cttccatgcc aagctcgtcg tcatggattt cgacgggttt
      361 tcgaccgagg tcgccgtcta caagaacaaa gcgctatacg atgacatcgt gacccgcggc
      421 atgccgctcg gcaacgacgg caaggaattg gcgagcccga tgggggtctt tggcgccctc
      481 attccaaaag gtgcaaagaa cgttacggtg gccaaggatt tcttgaaata cgcggtccag
      541 ccgaacgtgt tgaacgagta tttgaagggc ggcctcggac gctgggtaat tccaatgcgt
      601 gaaatcgcga aagctgaccc gttctggttc cgtgaggaca agaaccgcac cgcttttgcc
      661 gaggagacgc tgacccatcc gacagtaccg ctctatcagg cattcaaccc ggcgtacgcg
      721 caggtcgact ccgagcacgt cttcagcatc gccatgctcg atgtcatgaa caacgggatg
      781 atcctggagc aggcggtcgg caaggccttc aagcgggccg aggagatctt cgcgaaatat
      841 ccgatcacgg aggcgtgaga gctaggaaag gcgggttagt cgctttctcg tgcggctcga
      901 ttgaggggac cgcgacgttc cacataacaa ctgccggcgg cgcccagttc cttcaagatt
      961 agcgctcgga caaccgtggc cgtgccgcaa ggtcaatggc atcgcttcca atcagccgaa
     1021 ggcgtaagcc tcatctcggc gacgccgctg ccaacgcgat cacaaatgtc cgttccggca
     1081 ggggtgaacc gaagcgggcg atccggtcag gtcgacctcg gcggtttaat cgccgtccca
     1141 ggaacggccg gcatcggcgc tacctcgccg ctggctgccg ggtcggcgaa ggacggctgt
     1201 ccacccgcat gggcggttcg ccgcgctgat ccttgaacgt cgcctatcac cgggtatagc
     1261 ggttatcgcg ccggcctagc aggagcggcc cacccacttg agccgatccc gcctaggcag
     1321 ccgagggtcg gctatgtgat cggtgatcag ccgctcgcac ggaggccggc cggcgcgtgc
     1381 tgtcgaccct cagcagaccc tcagccttca atgcggaacc gctcttcgtc tattcgcgcc
     1441 agaattttac ggcaacgacg gtatccgcgc gatatgaccg cgccgggtct cgggtatctc
     1501 gccgccatca caatcatcgt gggctacggc ttgcccgaag ctacatgagc atgaattcgg
     1561 cgacatccga caccgcgatc accgaggaat tcgatcccat ccatcggccg gatagatttc
     1621 tgcatctaat gccgccgaag cccgacacag cgccttgcgt gggacgcgtc catagatgta
     1681 ggccggaacg cggaggctca tctcggttcg gaccttgtcg agggtctggg tcgggaggtg
     1741 ggtgcccggc gaccgcgtgc gcttgatccg gatgaaatcg ccgggcttta ctcgaagctg
     1801 ccagccgatc accattcgcg cggcgatttg gtgctggtcg cggggtgggc acgatgcgcg
     1861 gatccggtcg gctttgcggc gctggccgcc tgatcgcgcg ttcagcggcc gtcgttctga
     1921 tcctcggctt cggttccgtt gcagtgctcg gcgacgagcc ggcgaagccg atgacgatcg
     1981 tcgtattcgg cgatagccag gcgcagggcc tcgcggacgg gttgtcgcgc gtgctcgtcg
     2041 aagacgcgcg ctaccgcgtg ctcaaccgc
//
LOCUS       DATNOI010000640        26800 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_325595,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000640 DATNOI010000000
VERSION     DATNOI010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 26800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26800
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325595"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="VN968_25810"
     CDS             complement(<1..1149)
                     /locus_tag="VN968_25810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HXL32681.1"
                     /translation="MRQIVPPEWNRKIRHWLLDFDARIDSTLFSSGKGARELYERYST
                     FMDRFYVGRWKRWVFIEPASEAATIGLGGLILMLALAVPAFRETADDDWLKKSELAVT
                     FLDRYGNPIGGRGIKHNDSIPLEDFPDNLIKATLATEDRRFYDHFGIDIAGTARALLT
                     NAQAGGVRQGGSSITQQLAKNLFLSNERTIERKVNEAFLAIWLETRLSKNEILKLYLD
                     RAYMGGGTFGVDGAAHFYFNKSARDVNLAEAAMLAGLFKAPTKYAPHINLPAARARAN
                     VVLDNLVDADFMTEGQVFGARRNPATAVDRRDENSPNYYLDWAFDEMRKLVDTFPRSY
                     VERVFVVRTAIDMNVQRAAEDAIENQLRQFGRDYHATQAATVVADLDGG"
     gene            1433..1945
                     /locus_tag="VN968_25815"
     CDS             1433..1945
                     /locus_tag="VN968_25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HXL32682.1"
                     /translation="MTVRSKPPSDQEGFFWKTKTLEEMSSVEWESLCDGCARCCLEKL
                     EDEDTGQIYFTHVSCKLLDAGLCACKDYANRSDKVPDCVRLTPANVRTLNWLPPSCGY
                     KLVAEGRDLYWWHPLISGDPNTVHQAGVSVRGRVRGTENEIADADLEDHIVQWPALLP
                     KRARLKKRPA"
     gene            2089..3666
                     /locus_tag="VN968_25820"
     CDS             2089..3666
                     /locus_tag="VN968_25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HXL32683.1"
                     /translation="MNKFERQGRQPADQQVLPEAIEGDLPEMAAEVIDLGDAPPLAPR
                     APLTQSEVRTILISLMLTMFLAALDQTIVATALPTIGRQFQDVSNLSWVITAYLLAST
                     AVAPVFGTLADIYGRRAMIVTALSLFVAGSILCALAPNMPVLILARGLQGLGGGGIMP
                     VVQTVISDVITPRERGQYQAYFSGVWMAAGIAGPVIGGVFAEHLHWSMIFWINVPLGL
                     ASLAMLLPKMGKIPVFHRRRQVDWLGGVLMMAAAVVFMLVLTWGGNRYLWLSPVILAM
                     VGASFALALAFVWHARNAEEPFLPLSLMGGTVAPYAMASGGFALGAILGLTVHLPLYY
                     EVVYHLSASEAGLALIPLAAVSTGGAAIAGRTMARAKHYKRVAIIGTGCAALAGCVLA
                     LTAPPLWVLLVLLSVFALGLGTAFPISVVSLQNSVARSQVGTITGAMNFFRALMASFT
                     VAAFSAILLMALGADISLGGEHRGAVSSIPVADMITAFRYVFGAAAALLACASLCMIL
                     MEEKPLAGPATRVEMAE"
     gene            3793..4014
                     /locus_tag="VN968_25825"
     CDS             3793..4014
                     /locus_tag="VN968_25825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32684.1"
                     /translation="MSKPTIEQTRMGAEGIAFCIARTLIERDPSLKAPMRANLRKMWE
                     LLEEREDHAAADMVDTMIKALNDPAFFKP"
     gene            complement(4021..4410)
                     /locus_tag="VN968_25830"
     CDS             complement(4021..4410)
                     /locus_tag="VN968_25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXL32685.1"
                     /translation="MSVLVLVVDDEPDVEALFRQQFRRDLRAQRFVMDFAISAADALA
                     RIASTIEQSLILILSDINMPGMTGLEMLPKVKEMRPDVPVIMITAYGDPETKRKAIEG
                     GAEGLLTKPIDFTLLREEIDARLERTG"
     gene            complement(4413..7547)
                     /locus_tag="VN968_25835"
     CDS             complement(4413..7547)
                     /locus_tag="VN968_25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXL32686.1"
                     /translation="MVTDLRRRIADLEQRLQSGLAEHEQVIVQQDATALDNARLLTEL
                     RAAQDRQTGSAEILRAIANASGEAERSLHQIAETTARLFDAPSVTLHIAEGDDWGPTI
                     RVGDSSKRIGMEVSAAQLRIGGHNLPGTVLLQNRQIHIPDINNVDPQFADWPGLPPAR
                     AAGTHAIAGTPLRREDKAIGVLIVYRDRPLPFTADELALQQSFADQAVIAIENARLFN
                     EVQARTGDLSEALQQQTATAEVLKVISRSAFDLQKVLDTLSESAARLCEADIVVIHRS
                     IGDSFQGAASFGLPEDHHRAVMDIAHKPGRGSLGARVLFENAPVLIDDAECDPEYTFG
                     SFQRRIGMRSMLGVPLMREGAAIGLLLIFRKRVQPFTKRHVDLATTFADQAVIAIENV
                     RLFDEVQARTEDLRESLQFQTATSDVLKVISSSPDSLQPVLDVIVDTSRHLCGTETST
                     IFLLRDQKFHIAAASGAMPEYLEFLQANPIALDQAGSSTSRAAREKRTVHIPDIGADP
                     EFGQGPMAVGQRRALLSVPLMHEDTAIGVITMRQSHLTPFTERQIEVVKTFADQAVIA
                     LSNVRLFEEVQVKTRDLTESLQQQTATADVLKVISRSAFDLQTVLDTLVESAARLCEA
                     DMAAITRQHGDEYFRAGSYGFASQFMDYVRDIPVKPERGTITGRTLLEGKVIHVPDVH
                     ADPDYTFSEAQRLSGDPRTFLGVPLLRKGHPAGALLLLRRNMRPFTDKQIELVKTFAD
                     QAVIAIENVRLFDEVQARTKELAASLDDLRTAQDRLIQTEKLASLGQLTAGIAHEIKN
                     PLNFVNNFAALSAELIDELNDLFKPAAFDGNTRKEVDELTQTLKDNLEKIVQHGKRAD
                     SIVKNMLLHSREGSGEHRPSDINALLDESLNLAYHGARAEKPQFNVTLQRDFDASAGE
                     IELFPQEITRVFLNLISNGFYAVSKRRAENGNSGFEPVLSATTRNLGETVEIRIRDNG
                     TGIPTEVKEKMFNPFFTTKPAGEGTGLGLSMSHDIIVKQHGGRIDVDTEPGQFTEFRI
                     ILPRTSNFSNKNRG"
     gene            8115..9698
                     /locus_tag="VN968_25840"
     CDS             8115..9698
                     /locus_tag="VN968_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32687.1"
                     /translation="MIKMLSTAFTGAVLISLAQPALGQSDDKTLGAVHFETSCKPEAQ
                     KLFDRGMLYQHSFWYRASQNQFEDVLKADPECGIAYWGTALSLLWNPHVAPPAKNLAE
                     GAAAIAKGKSVGAKTQRERDYLDALGAMYADYDKVDHRTRMQAYAKAMEQLAQRYPDD
                     DEAQIYYALALNTSASPADKTYASQLKGAAILEPIAIRQPQHPGVAHYLIHLYDYPPI
                     AEKGLDAARRYAKVAPAAAHAQHMPSHIFTRVGYWKESIASNVESSRVARADNEGGDQ
                     LHAMDYLVYAYLQLGQDKEAAAVIDEMNAVSGFAETFIAGPYALAASPARYAVERGDW
                     KAAAALQVRPSPLAQVQAVTLFARALGAARSGNPEAAKADIAKLAELRDKLRDAKDAY
                     WSEQVDIQRQVATAWLLCAEGKRDEALTAMSAAADAEDKTEKHPVTPGVPRPARELYG
                     VMLLESGNAKDALAAFEATIRKEPNRLGAYAGAAKAAEKSGESAKAQEYYSKVVAIAD
                     AADDSRTEVADARAFLTKR"
     gene            9723..10103
                     /locus_tag="VN968_25845"
     CDS             9723..10103
                     /locus_tag="VN968_25845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32688.1"
                     /translation="MRAFFLPIVLSALATPAGAQSLQVTGYSGYLGEWELTATVTENI
                     SGRTNEYSGPLTMKHVGLCTQDGPEEKTGEMRLQISALPPRLNATLLVAGVECTYSGR
                     LSDSYTGTMACPGRGAVPLRLWVK"
     gene            complement(10132..11766)
                     /locus_tag="VN968_25850"
     CDS             complement(10132..11766)
                     /locus_tag="VN968_25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HXL32689.1"
                     /translation="MKLGTAVAEIMKREGIEILCGYPVNHLIEYAANADIRPVMVRQE
                     RIGLHMADAISRVTSGRTIGAFCMQHGPGAENAMGGVAQCFGESVPVLVLPMGYARRL
                     ANIEPNFNSSQAMKAFAKSAEPINIASEVGNIFRRAFTKLKNGRGGPVIVEIPADTWN
                     EEVPEPLNYTPVLRTRYGADPVHVREAAALLIGAKHPVIYAGQGVHYAQAWPQLKRLA
                     EKLAIPVTTSLGGKSSFPETHPLALGSGGLAVPRAVPKFLGQADVIFGIGCSFTETSF
                     GIAMPKGKTIIHSTLDPAHLNKDVEARIGLVGDAGLVLDALLEEISKSVTTDRDASAV
                     AAEIAASHKEWLAKWMPKLTSNDAPLNPYRVLWDLQHTVDIKNTIITHDAGSPRDQLS
                     PFWKPVEPLSYIGWGKTTQLGYGLGLAMGAKLAKPDKLCINVWGDAAIGFTGMDFETA
                     VRERIPIMSILLNNFSMAIELKVMPISTEKYRSTDISGDYAAMARAFGGHGERVTRPE
                     DIIPAIRRGIAKTREGIPVLLEFITSKETEVSRPGT"
     gene            12301..13884
                     /locus_tag="VN968_25855"
     CDS             12301..13884
                     /locus_tag="VN968_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXL32690.1"
                     /translation="MMAARKFILGSAAGLLAIGGAQAADLPVKAKAVEYVRVCSLYGA
                     GFFYIPGTDTCIKLGGYLRVDTTFNGGIHGQPAWSGDLGQQNRYRDYFASRSRMALTI
                     DTRTATEYGVVRTFGQADFQFSTLGGSTTNPNSVNQTAPVGGVQLLDTPGGGYVAVEY
                     LFIQFAGFTFGKSSSAYSTPWNGFPGNINSSLLGGNNTDTGVNNIQYTAQFGNGVSAT
                     IGLDDPTVWDRTIVANLSLPLSATEGAANAYAGTHAPDVVGNIRIDQAWGLFQISAAA
                     HEVSGSYNVLNTAAVPAGATGLAGAAPTALSEISGHPETKWGGSVMAALNIKNIPTGA
                     GDDIKIDASYAKGDTKNVIATSGNSPSFAMFGGSNTAYQSVGFGLTSDAVYLPVANGG
                     DGRLHLTDAYGVRGAFNHNWDPYWSSSLFGSYSAVRYDGTAKANICTNYTTAGKAVGG
                     VIAFTCNPDYNVSQLGFLTRWTPVKNLTFSAEVQWFHLDQKFSGVATLGPTAPKPSAV
                     YEFKDQDTFLLQVRAQRNF"
     gene            14109..14273
                     /locus_tag="VN968_25860"
     CDS             14109..14273
                     /locus_tag="VN968_25860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HXL32691.1"
                     /translation="MRHVFSKFLRDQSGATAIEYCLIAVGIAIVIIAAVNGIGTTLNT
                     GFASVNSSLK"
     gene            14297..14512
                     /locus_tag="VN968_25865"
     CDS             14297..14512
                     /locus_tag="VN968_25865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32692.1"
                     /translation="MQELLFGTWSPQVIAYSQQYPWRVFAAVLVAIPLLDFMFLKGRL
                     SGLGDGGDFGGSGGSDCGDGDGGGCGD"
     gene            complement(14521..14757)
                     /locus_tag="VN968_25870"
     CDS             complement(14521..14757)
                     /locus_tag="VN968_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32693.1"
                     /translation="MPWSTPFDPPIRLHGGGTLTTLQHAADHIMKLPEDVQQQELWQI
                     AVENLINAAETGGGWLMFARIGVMRALNGDGKGR"
     gene            complement(14840..15799)
                     /locus_tag="VN968_25875"
     CDS             complement(14840..15799)
                     /locus_tag="VN968_25875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HXL32694.1"
                     /translation="MAPRANWKGFLRLSLVTCPVALYPATSDTEKVSFNQINRKTGHR
                     IKYAKVDADTGEEVAADDIMKGYKVDTDTYIEVSKDELDDIALESTRTIEIDEFVPKT
                     DIDNRYLIRPYYLVPDGKVGHDAFAVIRETIRSMNKVAIGRVVLTNREHIISLEPLEK
                     GLMGTLLRYPYEVRSEKEYFDDIQDVKVTKDMLDLARHIVEKKSGSFEPDRFEDRYES
                     ALVDLINQKRNGIRTTAKAAPKSSGNVINLMDALKRSLASEKQSAPPVAAKGKKPRKA
                     ASGQREMLLPISGKRTAKEEAKAQEPKRAEKPVRAPARSKKAG"
     gene            16060..16212
                     /locus_tag="VN968_25880"
     CDS             16060..16212
                     /locus_tag="VN968_25880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32695.1"
                     /translation="MATRPQRQIVETPTEARQAEPGPSVLALLTVSTGLAVLILGAIW
                     FAFFRI"
     gene            16742..17074
                     /locus_tag="VN968_25885"
     CDS             16742..17074
                     /locus_tag="VN968_25885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32696.1"
                     /translation="MHDKPELKRETDLDETILDEASFANAVPESARLALGILNRRADQ
                     RCLVDGIAQLNRGGGGATQMGHRDGNDIGARSPRRHDNAGGEHDVDQAVVVSERRAEF
                     EENNGSGG"
     gene            17520..18191
                     /locus_tag="VN968_25890"
     CDS             17520..18191
                     /locus_tag="VN968_25890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL32697.1"
                     /translation="MRLLVVEDDPDLNRQLTTALTDAGYVVDRAFDGEEGHYLGDSEP
                     YDAVVLDIGLPKMDGISVLEAWRRNGRAMPVLILTARDRWSDKVQGFDAGADDYVAKP
                     FHLEEILARIRALLRRSTGHAQSELTCGPVSLDTRTGRVSVSGNPIKMTSHEYRLLAY
                     LMHHTGRVVSRTELVEHLYDQDFDRDSNTIEVFVGRIRKKLDVDIIQTVRGLGYLLTP
                     PSPRA"
     gene            18282..19658
                     /locus_tag="VN968_25895"
     CDS             18282..19658
                     /locus_tag="VN968_25895"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXL32698.1"
                     /translation="MRGSSLATRLFLSATAWVVVILLITGVVLSSVYRDATERAFDRR
                     LNLYLRTLIAEVATPDEPPDHQFQSLGEPLFELPLSGWYWQITRTDSEKAEVRASRSL
                     WDKKLPKLEDHGADLTAAGIRLGYVDGPEGQNLRMVERPVDLGADGKFLVSVAGDASE
                     IFDETRSFDYYLGGTFVALSIVLVLTTIFQVRFGLAPLKRISESIADIRSGRAERLEG
                     EFPVEIAPLARETNALIDANREIVERARTHVGNLAHAIKTPLSVIVNEASAHGADPFA
                     TKVLEQADVMRDQVAHHLERARIAARLTIIGTVTEVAPAIEALRRTMEKIHRGRGIAI
                     AVEADPQAKFRGERQDLEEMAGNLVDNACKWAASQVFIEVLVERPVKAGAGPMLRILV
                     DDDGRGLSAAERAQVSRRGQRLDESKPGSGLGLSIVVDLAALYGGSLSLGSAPIGGLR
                     AELVLPGV"
     gene            19812..21212
                     /locus_tag="VN968_25900"
     CDS             19812..21212
                     /locus_tag="VN968_25900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32699.1"
                     /translation="MSQTSIERLRDYLAQLPPQAQALLMREFERAIERGEDATVATLV
                     LEQLRKVVRGTDEETRARTDDPARLLFRPLEPFLIDSNFPARPGQIRRASLPPVWQWL
                     IRDGAPDQAREFEAALTLVRETGTTSGLEAAIRKFQLAAADAIYKIAMPVPGEDKQRA
                     LSRVGPPNVIEDLLPIGSVLQAREALDGLNGRLPSNLRILADAQIATVSAALNVPALQ
                     TPQTLPFALSLIIQRLTAPWQIIRLAIKMAASDDEIRVAATPYGMAVTIALHDLYFLA
                     ACLRTDIKRGHFDDVAEQLKSLHDGVRGLRTELDLRNDSSWGKQLTSIRADISNTLQS
                     EIDSVPGRVRRLLRQRADKDISVGAKIDTSEVDETAALIDFVAVCRTYASELAINEVT
                     LRTYSDLQQYVEKSTEALVQSLRGGDAKARAYRQMQVKAAVRFCEVLFGHDYASLMSR
                     AAENALTGERKPSRAG"
     gene            21341..21610
                     /locus_tag="VN968_25905"
     CDS             21341..21610
                     /locus_tag="VN968_25905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32700.1"
                     /translation="MNSIIYFVELENRVISATYRNLMIGAKVVIVDKTSGAQLSDPVT
                     TIASPVPNGSLRIRLPEAVKPGAYYLKALNGHGEQAAQSVEFHVG"
     gene            21745..22848
                     /gene="ccmI"
                     /locus_tag="VN968_25910"
     CDS             21745..22848
                     /gene="ccmI"
                     /locus_tag="VN968_25910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HXL32701.1"
                     /translation="MTLWFVFALMTAAAIFAVLWPLGRGTRPQNDGSETVVYKDQLAE
                     IERDVSAGLIGRSEADAARVEISRRLLAAADHQRAPAVVSSIKLRRSAAITALVGVPI
                     VAVAFYLPLGSPQLGDFPLAQRTHAPDVTQPLDNLVAQVEQHLAKNPTDGRGWSVLAP
                     VLARLGRYDDAVRAYHNSITYNGDSATRRADLGEALAAAAGGVVTSEAKAEFERALAL
                     NPDEVKARYFLGLAAEQDGRTAEANSIWGAMLAKAPPDAPWRPLVQAALARVGGTTAP
                     ALSDEAVAAARDMNEADRGTMIRGMVERLASRLKQNGDDVDGWLRLVRAYVVMGDRDK
                     ARGALTDARQAVANDAERLRQLNEGLKNLGLDG"
     gene            22927..23481
                     /gene="ccmE"
                     /locus_tag="VN968_25915"
     CDS             22927..23481
                     /gene="ccmE"
                     /locus_tag="VN968_25915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HXL32702.1"
                     /translation="MTRKQRRLTMIGGSLAVLAVAAALVLNAMRDSIVFFSTPAMVAE
                     KHVPAGKRFRLGGLVQPGSLLRGDNLAVAFRVADGSAALPVSYKGILPDLFREGQGVV
                     AEGALDSSGVFEADTVLAKHDETYMPKDVADALKKQGHWRDDYGAKPDYGAKSAITQG
                     AARAAQSAPQADRPAPEPNSGALQ"
     gene            23478..25460
                     /locus_tag="VN968_25920"
     CDS             23478..25460
                     /locus_tag="VN968_25920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HXL32703.1"
                     /translation="MIAETGHYALVLALALALIQSTLPILGARWHDPALMNVARSTAL
                     AQLLFVAASFAALVTLHVTSDFSVVNVFENSHSLKPLIYKITGVWGNHEGSMLLWVSI
                     LALFGGLVAAFGNNLPLSLRAHVLAVQAWIASAFYLFILITSNPFLRIDNPPIEGRDL
                     NPVLQDIGLAVHPPMLYLGYVGFSISFSFAVAALLEGRIDAAWARWVRPWTLVAWIFL
                     TLGIAMGSYWAYYELGWGGWWFWDPVENASLMPWLAGTALLHSAVVMEKRNALKVWTI
                     LLAILTFSLSLLGTFLVRSGVLTSVHAFATDPTRGVFILLILCIFIGGSLTLYAWRAS
                     ALKQGGLFAPISREGALVFNNLFLTTACATVFIGTLYPLALEVLTGDKISVGAPFFNL
                     TFAPLFLPLMIAVPFGPLLAWKRGDLLGAAQRLIAAGIAALVAIAVLWAWTRGGSAFA
                     PLAIGLAVFVIAGALCDLAERIGLFRMPFATVLRRARGLPRSTWGTTFAHAGVGVALI
                     GIVCETTWNSEFIGSMKPDDVAKIAGYELKLDGLTQRQGPNFREMLAQFTVTLDGKLL
                     SAMTPSKRNFPARGSSTTEAALLTRGASQLYISLGDTTADGAIAVRIYHKPLVLLIWW
                     GPVLMAFGGVLSLSDRRLRVGAPKPAKVLRGLQAAE"
     gene            25465..25977
                     /locus_tag="VN968_25925"
     CDS             25465..25977
                     /locus_tag="VN968_25925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HXL32704.1"
                     /translation="MKPILACVFAMVVMMGSSAAYAVQPDEIMSDPAMETRARDLSRE
                     LRCMVCQNQSIDDSEAPLARDLRLLVRERIAAGDSDGQVIDFLVARYGEFVLLKPRLK
                     PHTLLLWLLPPLALAGGGFVLWTHGRRRSRSAAAEDQSLLRLTAEEEARLERLTAAEP
                     AADKPAQGSR"
     gene            26196..>26800
                     /locus_tag="VN968_25930"
     CDS             26196..>26800
                     /locus_tag="VN968_25930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXL32705.1"
                     /translation="MTDRPTDLSSLPSYQTPRRSLFSARKFALMASVVAGLGVAVYGF
                     SPSSRPVDVFSSPAHAQVNNEVRKVERPVGFADIVERVKPSVISVKVNINEKIAKDDS
                     TNNNDDSPFQPGSPMERFFRRFGGPDGMPPGMRGGPRGRGAVTGQGSGFFISPDGYAV
                     TNNHVVDGADKVEVTTDDGKTYTAKVIGTDARTDVAPIKVEG"
BASE COUNT         4832 a         8203 c         8462 g         5303 t
ORIGIN      
        1 gccgccgtcg agatcggcga ccacggtggc ggcctgggtc gcgtgatagt cgcggccgaa
       61 ctggcgcagc tggttttcga tggcgtcctc ggcggcgcgc tgcacgttca tatcgatcgc
      121 ggtgcggacc acgaagacgc gctcgacata ggatctcgga aaggtatcga ccagcttgcg
      181 catctcgtcg aacgcccagt cgagataata attcggcgaa ttctcgtcgc ggcgatcgac
      241 cgcggtggcc ggattgcgcc gggcgccgaa cacctggccc tcggtcatga agtcggcatc
      301 gacaagattg tcgagcacca cattggcgcg ggcgcgggcg gcgggcaggt tgatgtgcgg
      361 cgcgtatttg gtgggcgcct tgaacaggcc ggccagcatc gcggcttccg cgagattgac
      421 gtcgcgcgcc gacttgttga aatagaaatg cgcagcgccg tcgacgccga aggtgccgcc
      481 acccatatag gcgcggtcga gatagagttt cagaatctcg ttcttggaaa gccgcgtctc
      541 gagccagatc gccagaaacg cctcgttcac cttgcgctcg atggtgcgct cgttgctcag
      601 gaacaggttc ttggcgagct gctgggtgat ggacgagccg ccctgccgca cgccgccggc
      661 ctgcgcattg gtcagcagcg cacgcgcggt gcccgcgata tcgatgccga aatggtcata
      721 gaagcggcgg tcctcggtgg cgagcgtggc cttgatcaga ttgtcgggaa aatcctcgag
      781 cggaatcgag tcgttatgct tgatgccgcg gccgcctatc ggattgccgt agcggtcgag
      841 gaacgtcacc gcgagttcgg attttttcag ccagtcgtcg tcggcggtct cgcgaaaggc
      901 cggcaccgcc agcgccagca tcaggatcag gccgccgagc cctatcgtgg cggcttcgga
      961 agccggctcg atgaataccc agcgtttcca gcggccgaca tagaaccggt ccatgaacgt
     1021 cgagtagcgc tcatagagct cgcgcgcgcc cttgccggaa gagaacagcg tcgagtcgat
     1081 gcgcgcatcg aagtccagca gccagtgccg gatttttctg ttccattctg gtggtacgat
     1141 ctggcgcacc gggacccttg gtcagttcgc agcgcccagg cacccggctg ggcaccgctt
     1201 aagacgtctt gcgggaatgt tgcagcaaac ccaaggcgct tttgcgtcgc tggggactca
     1261 aggcctctat ctagccgagt ggccggtaca aaccaatggt gcggctacga ttttgaacgg
     1321 caggactaac accgcccacc tgcttgtgct accgcggaaa ccttgcagcc tgcaccattg
     1381 ccgtgcagcc ctttttcggg gcctgagcta acgcgaggaa acgtcaggat tcatgaccgt
     1441 ccgatccaag ccgccctcgg atcaagaggg attcttttgg aaaaccaaga cgttggagga
     1501 gatgtctagc gtcgaatggg aaagcctgtg cgacggctgc gcgcgctgct gtctcgaaaa
     1561 gctcgaggac gaggataccg gccagatcta tttcacccac gtttcctgca agttgctgga
     1621 cgccggccta tgtgcctgca aagactacgc gaaccggtcc gacaaggttc cggactgcgt
     1681 ccggctcacg ccggcaaacg tccgcacgct gaactggttg ccgccgagtt gcggctacaa
     1741 gctggtggcg gagggccgcg atctctattg gtggcatccc ctgatttccg gcgaccccaa
     1801 tacggttcac caagccggcg tctcggtgcg cgggcgggtg cggggaaccg agaacgagat
     1861 cgcggacgcc gacctcgagg accacatcgt gcaatggccg gcgctgctgc cgaagcgggc
     1921 gcgtctgaag aagcggccgg cctgaccgat cacgcatttg cgattgcctc ttcgcgggat
     1981 gcacccatgc gcctaacccg ctgccgtagc atggatatgc tgcttaaata gcggatacag
     2041 cgattccacg cggccccccg tcgcgcacct cattaaacgc acgtccgcat gaacaagttt
     2101 gaacggcagg gccgtcaacc ggctgaccaa caggtgttgc ccgaagccat cgagggcgac
     2161 ctgccggaaa tggcagctga ggtcatcgat ctcggcgacg cgccgccgct cgccccacgg
     2221 gcgccgctta cgcaaagcga agtccgcacc atcctgatca gcctgatgct gacgatgttt
     2281 ctggccgccc tcgaccagac catcgtcgcg accgcgctgc cgaccatcgg ccggcagttc
     2341 caggatgttt ccaacctgtc ctgggtgatc acggcgtatc tgctggcttc caccgcggtg
     2401 gcgccggtgt tcggaacact cgccgacatc tacggccgcc gcgccatgat cgtcaccgcc
     2461 ctcagcctgt tcgtcgcggg ctcgatcttg tgcgcgctgg cgccgaacat gccggtgctg
     2521 atattggcgc gcgggctgca ggggctcggc ggcggcggca tcatgccggt cgtgcagacg
     2581 gtcatttccg acgtgatcac gccgcgcgag cgcggccagt atcaggctta tttcagcggc
     2641 gtatggatgg ccgcgggtat cgccgggcct gtcatcggcg gtgtgttcgc cgaacatctg
     2701 cactggtcga tgattttctg gatcaacgtg ccgctcgggc tggcttcgct ggcgatgctg
     2761 ctgcccaaga tgggcaagat cccggtgttt caccgccggc gccaggtcga ctggcttggc
     2821 ggcgtgctga tgatggccgc cgctgtcgtg ttcatgctgg tgctgacctg gggcggcaac
     2881 cgctatctct ggctctcgcc cgtcattttg gcgatggtcg gagcgtcatt cgcgctggcg
     2941 ctggcgtttg tctggcatgc ccgaaatgcc gaagagccgt tcctgccgct gtcgctgatg
     3001 ggcggaacgg ttgcgcctta cgcgatggcc tccggcggct tcgcgctggg cgcgatactg
     3061 gggctgaccg tgcatctgcc gctttattac gaggtggttt atcatctcag cgccagtgag
     3121 gccgggctcg cgctgattcc gctggcagcc gtttcgaccg ggggtgccgc gatcgccggc
     3181 cgcaccatgg cgcgcgccaa acactataag cgggtcgcga tcatcggaac ggggtgcgcg
     3241 gcgctggcag gatgcgtgct ggcgctcacg gcgccgccgc tatgggtgct gctggtgcta
     3301 ttgtcggtgt tcgcgctggg ccttggcacg gcattcccga tcagcgtggt ctcgctgcag
     3361 aattcggtgg cgcgctccca ggttggcacc atcacgggcg cgatgaattt cttccgcgcg
     3421 ctgatggcgt ccttcacggt ggcggcgttc tccgcgatcc tcctgatggc gcttggtgcg
     3481 gatatttcgc tcggcggcga gcaccgcggc gcggtgagtt cgattcccgt cgcagacatg
     3541 atcacggcat tccgctatgt gtttggcgcc gccgcagcgc tgctcgcctg cgcgtcgctg
     3601 tgcatgattc tgatggagga aaagccgctg gccggcccgg ccacgcgggt ggagatggcg
     3661 gagtagggcg ccagacccct tgcgtgctgt ccgccctcca gccttggcta ttcatgagcc
     3721 gcgatttgat ctaacagcgg cgccaagaag tcagacaccg aaattcgagc gtaattcgag
     3781 aaggagccgg ccatgagcaa accgaccatc gagcagaccc ggatgggtgc cgaaggcatc
     3841 gccttctgca tcgcgcggac cctgatcgaa cgcgacccct cgctgaaagc gccgatgcgc
     3901 gccaacctgc gcaagatgtg ggagcttttg gaggagcggg aggaccacgc cgccgctgac
     3961 atggtcgata ccatgatcaa ggcgctcaac gatcccgcgt ttttcaagcc gtgagctggt
     4021 tcaacctgtt cgttcgagcc gcgcatcgat ttcttcccgc agcaaagtga aatcgattgg
     4081 cttggtcaac aagccttccg caccaccctc gatcgccttg cgtttggttt cgggatcgcc
     4141 gtaagcggtg atcatgatga cggggacgtc gggccgcatc tccttgacct tgggcagcat
     4201 ttcaaggccg gtcatgccgg gcatgttgat gtccgacagg atcaatatca gcgactgttc
     4261 gatggtgctg gcgatgcgcg ccagcgcgtc cgctgctgaa atggcaaagt ccatcacgaa
     4321 acgttgtgca cgcagatccc gccggaattg ctggcggaac agcgcctcga cgtcgggctc
     4381 gtcgtcgacc accaggacca atacgctcac gtctaacctc tgtttttgtt tgaaaaattg
     4441 cttgtccgcg gcaaaatgat tctgaattcg gtaaactgtc cgggttccgt atcgacgtct
     4501 atcctgccac cgtgttgttt cacgatgatg tcgtggctca tcgaaagccc gagcccggtc
     4561 ccttctcccg ccggcttcgt tgtaaagaac gggttgaaca tcttttcctt cacctcggtc
     4621 gggatacccg tgccgttatc gcgaatgcgg atctcaaccg tctcaccgag atttctggtg
     4681 gtcgcgctga ggaccggttc aaaacccgaa ttgccattct ctgctctgcg tttgctcacc
     4741 gcataaaatc cattggagat caaattcagg aacacgcggg tgatttcctg cggaaacaat
     4801 tcgatctcgc cggcgctcgc atcgaagtcc cgttgcaagg taacattgaa ctgcggcttc
     4861 tcggcgcgcg cgccgtgata ggcgagattg aggctctcat ccagcagcgc attgatatcc
     4921 gagggccgat gttcgccgga gccttcgcgc gaatgcagca gcatgttctt gacgatcgag
     4981 tcggcgcgtt tgccatgctg gacaattttt tcgagattgt ccttcagcgt ttgcgtcaac
     5041 tcgtcgacct ccttgcgcgt gttgccgtcg aaggctgccg gcttgaacag atcgttcagt
     5101 tcatcgatca gctcggccga gagcgctgcg aaattattga cgaaattgag cgggttcttg
     5161 atctcgtggg cgatccccgc cgtgagttgg ccgagcgagg caagtttttc cgtctggatc
     5221 aggcgatcct gcgcggtgcg caggtcgtcg agggaggcgg cgagctcctt ggtgcgcgcc
     5281 tgcacctcgt cgaacaggcg aacattctcg atcgcgatca cggcctggtc ggcgaaggtc
     5341 ttgaccaatt cgatctgctt gtcggtaaag ggtctcatgt ttcgacgcaa caggagaagt
     5401 gcgccagcgg gatggccctt gcgcagaaga gggaccccga gaaatgttcg gggatcgccg
     5461 cttaatctct gggcttccga aaaagtgtag tcaggatcgg catgtacatc gggcacatgg
     5521 atcactttgc cttcgagcaa agtacgtcca gtaatggttc cacgttctgg cttgaccggt
     5581 atgtccctta cgtaatccat gaactgtgaa gcaaagccgt atgagccggc gcgaaagtat
     5641 tcgtcgccgt gctgacgcgt aatcgccgcc atatccgcct cgcacaggcg tgccgctgac
     5701 tcgacaagcg tgtcgagcac tgtttgcaga tcaaacgccg agcggctgat aaccttgagc
     5761 acgtcggcgg tcgcagtctg ctgttgcagc gattccgtga ggtcacgggt cttgacctgc
     5821 acctcttcga aaagccttac gttcgagagg gcgataacgg cctggtcggc gaaggtcttg
     5881 accacctcga tctgtcgctc ggtaaagggc gtcaggtgag attggcgcat ggtgattaca
     5941 ccgattgccg tgtcctcgtg catcagcgga acggagagca gcgcccggcg ctgtccgacg
     6001 gccatcggac cctggccgaa ttcgggatcg gcgccaatgt cagggatatg tacggtacgc
     6061 ttctcacgcg ccgctcttga ggtcgacgaa ccggcttgat caagcgcgat cggattggcc
     6121 tgcaggaact ccaggtattc cggcatcgcg cccgacgccg ccgcgatgtg gaatttctgg
     6181 tcccttagca ggaatatcgt ggaggtttca gtgccgcaca aatgccggga cgtatccacg
     6241 ataacgtcga gcacgggttg cagcgaatcc ggcgaactgc tgatgacctt cagtacatcg
     6301 gatgtcgcgg tctggaactg cagcgattcg cgcaagtcct cggtgcgcgc ctgcacctcg
     6361 tcaaacaggc gaacgttctc gattgcgatc acggcctggt cggcgaaggt cgtggcaagg
     6421 tcgacatggc gcttggtgaa aggttgcact ctcttgcgga aaatcagcag gagaccgatc
     6481 gcggcgccct cgcgcatcag agggacgccc aacatgcttc gcatcccgat ccggcgctga
     6541 aaagagccaa aggtgtattc aggatcgcac tcggcgtcat cgatgagcac gggtgcattt
     6601 tcgaaaagga cgcgcgcgcc aagggatccg cgaccaggct tgtgagcgat gtccatgaca
     6661 gcgcggtggt gatcttcagg caaaccgaag ctcgccgcac cttgaaaact gtcaccaata
     6721 ctacgatgga tgacgacgat gtcggcctcg cacaaacgag ctgccgattc cgaaagagtg
     6781 tctagcacct tctgcagatc gaacgcggaa cggctgatga ccttgagcac ttcggcggtc
     6841 gcggtctgct gttgcagcgc ctcggagaga tcgcccgttc tggcctgcac ttcgttgaac
     6901 agccgcgcgt tctcgatggc gatcacggcc tggtcggcaa aactctgctg gagcgcgagt
     6961 tcgtcggcgg tgaaaggtag gggtcggtcg cgatagacga tgagaacacc gatcgccttg
     7021 tcctcgcgcc gaagcggcgt tcccgcgatg gcgtgagtgc ccgccgcacg ggcgggcggc
     7081 aagccgggcc agtcggcgaa ctgcgggtcg acattgttga tatcgggaat gtgaatctgc
     7141 cggttttgca gcaagaccgt gccgggcagg ttatggccgc cgatccgtag ctgcgccgcc
     7201 gaaacctcca tgccgatgcg tttcgaactg tcaccgacgc ggatggtcgg gccccaatcg
     7261 tcaccttcgg cgatatggag cgtgacactc ggcgcgtcga acagccgcgc ggtggtttcg
     7321 gcgatctggt gcaacgaccg ctcggcctcg cccgatgcgt tggcgatggc acgcaggatt
     7381 tcggcgctgc cggtctggcg atcctgggcg gcgcgcaatt cggtcagcag tcgggcgtta
     7441 tcgagcgcgg tcgcgtcctg ttggacgata acttgctcgt gctcagccaa accggactgg
     7501 agccgctgct caaggtcggc aattctccgc ctgagatcgg tcaccacgtc ctggggaagc
     7561 gcgatcattc actcaatcca tttcgcccgg tgcctcgcgg cctgcgagct ccaatagtat
     7621 gcggtcccgg ctcccggttg acgggtaacg ccatttgccc gtcaccgggc cgtacccgat
     7681 tattccattc cggacggatg cgatccagcg ccatcaggcc aattttgccc ggtcggggcg
     7741 ccaatactct caaatatcat gagcaaccaa agccttcggg gttacttccg atagcagatc
     7801 ttaaccgcaa cggcccgcag ccgcttgatc cgggcgttat tcttcgacgt cgagaccgag
     7861 ggccagccgc ccactaaaac ggagagctgc ggtcgatgga accgcgccct gaacgtcgcg
     7921 gaacaaccgc tccagcccgt ggcggaggag ttggaaaaca gacttgttga ctaacgcgcc
     7981 gaccggtatc agcatggttg cgcataccgc gattgagcac ctgccttagc gggcctcgcg
     8041 aaaatgcctg attgcccgaa aacgccgagg gtcgttgatc gcaggattgc gcgtcctgga
     8101 ccgggagcat acccatgatc aaaatgttga gcacagcttt cactggagcc gttttgattt
     8161 cgctggcgca gccagcgctc ggacaatcgg atgataagac cctcggcgcg gtgcatttcg
     8221 aaacatcgtg caagccggag gcgcagaaac tgttcgaccg aggaatgctt taccaacact
     8281 cattctggta tcgcgcgtcg caaaaccaat tcgaggatgt gctgaaggcc gatccggaat
     8341 gcggcatcgc ctattggggc accgcgctga gcctgctgtg gaatccacac gtcgcgccgc
     8401 ccgcgaagaa tctcgccgag ggtgccgccg cgatcgcaaa gggaaagagc gtcggcgcaa
     8461 agacccagcg cgagcgtgac tacctcgatg cgctcggcgc catgtacgcc gactacgaca
     8521 aggtcgatca ccgtacgcgc atgcaagctt acgccaaggc catggaacaa ctagcacagc
     8581 gctatcccga cgatgacgaa gcgcagatct attatgccct cgccctgaac acctcggctt
     8641 cgccggccga caaaacctac gccagccagc tcaagggcgc ggccatcctg gagccgatcg
     8701 cgatacgcca gccgcagcat cccggcgtgg cgcattactt gattcacctt tacgactatc
     8761 cgccgatcgc cgagaaaggc ctcgatgccg cccgacgcta tgccaaggtc gcgccagccg
     8821 cggcacatgc ccagcacatg ccctcacaca tttttacgcg cgtcggttat tggaaggagt
     8881 cgattgcttc caacgtcgaa tcgtcgcgtg tcgcgagggc ggacaacgag ggcggtgatc
     8941 agctgcacgc catggattac ctggtctatg cctatctgca gctcgggcag gacaaggaag
     9001 ccgcagccgt tatcgatgag atgaacgcgg tcagtggatt cgccgagact ttcatcgccg
     9061 ggccatacgc gctggccgct tcgccggcac gctacgcagt ggagcgtggc gactggaagg
     9121 ctgcggcggc gcttcaagtc cgtcccagcc cgctggctca ggtgcaggcg gtcacccttt
     9181 tcgcgcgagc gctaggcgca gcccgctcgg gcaatcctga agccgccaag gctgatatcg
     9241 ccaagctcgc cgagctgcgc gacaagttgc gcgatgccaa ggacgcctat tggtcggagc
     9301 aagtagatat tcagcggcag gtcgcgaccg cctggttgct ctgtgccgaa ggcaaacgcg
     9361 acgaggcact gactgccatg agtgccgccg ccgatgccga ggacaagacc gagaagcatc
     9421 cggtgacgcc gggcgtgccc aggccggcgc gcgaactcta cggggtcatg ctgctcgaga
     9481 gcggcaatgc aaaggacgcg ctcgccgcct ttgaagcgac gataaggaag gagccaaacc
     9541 gcctcggcgc ctatgcgggc gcggcgaagg ccgccgaaaa gtccggtgag tccgccaagg
     9601 ctcaggagta ttacagcaag gtcgtcgcga tcgccgatgc tgcagatgac agtcggactg
     9661 aggttgccga cgcgcgcgcg ttcctgacga aacgctaagg gacatccgaa agaaagcacg
     9721 tcgtgagagc attctttcta ccgatcgttc tgagtgctct ggcgacgccg gccggcgcgc
     9781 aatcgcttca ggtcaccgga tactcgggct atctcggcga gtgggagttg accgccaccg
     9841 tcacggaaaa catatccggc cgaaccaacg aatattccgg gccattgacg atgaagcacg
     9901 tcggtttgtg cacccaggat ggcccggagg agaagaccgg tgaaatgcgt cttcagatat
     9961 cggccttacc gccccggttg aatgcaacgc ttttggtcgc cggggttgaa tgcacttaca
    10021 gcgggcgctt gtcagactcc tataccggca cgatggcctg ccccggtcgg ggggcggttc
    10081 cgctcaggct atgggtgaaa tagtattgct ggcgtcaatg gccgcggctg ctcaggtccc
    10141 cgggcgcgaa acctccgtct ccttgctggt gatgaattcc agcagcaccg ggatgccttc
    10201 cctggtcttg gcaatgccgc gccggatcgc cgggatgatg tcctcgggcc tcgtgacccg
    10261 ctcgccgtgg ccgccgaagg cgcgcgccat ggcggcatag tcgccggaga tgtcggtcga
    10321 gcgatatttt tcggtcgaaa tcggcattac cttcagttcg atcgccatgg agaaattgtt
    10381 caacaggatc gacatgatcg gaatgcgctc gcgcaccgcg gtctcgaagt ccatgccggt
    10441 gaagccgatc gcggcatcgc cccatacatt gatgcagagc ttgtcgggtt tggcgagctt
    10501 ggcgcccatc gcaagaccca gtccatagcc gagctgggtg gtcttgcccc agccgatata
    10561 ggacagcggc tcgaccggct tccagaaggg cgacagctgg tcgcgcgggc tgccggcgtc
    10621 atgggtgatg atggtgttct tgatgtcgac cgtgtgctgc agatcccaca gcacgcgata
    10681 ggggttcaat ggcgcgtcgt tgctggtcag cttcggcatc catttggcga gccactcctt
    10741 gtgcgaggcg gcgatctcgg cggccacggc tgacgcatcg cgatcggtgg tgacgctctt
    10801 gctgatctct tccaacagcg cgtcgagcac cagtccggcg tcgccgacca gcccgatcct
    10861 ggcctcgacg tccttgttga gatgcgcggg gtccagcgtc gaatggatga tggtcttgcc
    10921 cttgggcatg gcgatgccga acgaggtttc cgtgaacgaa cagccgatgc cgaaaatcac
    10981 gtcggcctgc ccaaggaatt tcggtaccgc gcgcggcacg gccagaccac ccgaacccag
    11041 cgcaagcggg tgcgtctcgg gaaatgacga cttgccgccg aggctcgtgg tgacgggaat
    11101 ggcgagcttt tccgccagtc gtttgagctg cggccaggct tgcgcgtaat gcacgccctg
    11161 gccggcatag atcacgggat gcttggcgcc gatcaacagt gcggcggctt ccctgacatg
    11221 tacggggtcg gcgccatagc gggtgcgcaa gaccggcgtg tagtttagcg gctccggcac
    11281 ctcctcgttc cacgtgtcgg ccgggatttc gacgatgacc ggtccgccgc ggccgttttt
    11341 caatttggtg aaggcgcgtc ggaagatgtt gccaacctcg gacgcgatgt tgatcggctc
    11401 ggccgacttc gcaaacgcct tcatcgcctg gctggagttg aagttcggtt cgatattcgc
    11461 aagccgccgc gcatagccca tcggcagcac cagcacgggg acggactcgc caaaacattg
    11521 cgccacgccg cccatggcgt tttccgcgcc ggggccgtgc tgcatgcaga aggcgccgat
    11581 ggtgcgccct gaggtcaccc gcgatatcgc gtcggccatg tgcagcccga tgcgttcttg
    11641 ccgcaccatc accgggcgga tgtcggcatt ggcggcgtat tcgatgagat ggttgacggg
    11701 atagccgcag agaatttcga ttccctcgcg cttcatgatt tccgcaacgg cggtgccgag
    11761 tttcatggtg tttccttccc caaaaactgg ccgatgttgc actcggccga ttagggatag
    11821 ttggaacagg aaaccgtcga tcggtaaagc gcggcggcac ttcccccggg aagtgctgat
    11881 atgcacaacc gccgcgcgcg atacgggacc ggcttgcggc gtttcaaccg tcacatttcg
    11941 acgcttaccg gcacactgca cccggcgcgc tgaagcggag aagtccgttt cgctcgcctt
    12001 gcggccgagg ttttttcgcc ctggcgtttc tgccgacgag tgttggggga atcggctgcc
    12061 ctcgatccat catcgctcgc caaccagcac gagaatatct tgctaccttg atgtcgatac
    12121 gagggcgaaa cctggtccga tgttgcaaga tccgctttcg tgcgttgctc aaacatcaca
    12181 ccggctgggc gacctgcgca aaaggcaatc ttttcacgac cttgtcattc tttcggggaa
    12241 tttttccgtt actaagaata caacatgtac acgcatgaat catgatatta ggggaatcat
    12301 atgatggcgg ctcgaaaatt catccttggc tcggcggcgg gactcctcgc catcggcggg
    12361 gcacaggcgg ccgatcttcc ggtcaaggcc aaagcggtcg aatatgtgag ggtctgctcg
    12421 ctgtatggtg ccgggttctt ctacattccg ggcaccgaca cctgcatcaa gctggggggc
    12481 tatcttcgcg ttgacaccac gttcaacggc ggcatccacg ggcaaccggc ctggagcggc
    12541 gatctgggcc agcagaaccg gtatcgcgat tacttcgcct ctcgttcgcg tatggcgctg
    12601 acgatcgata cccgcaccgc gactgaatat ggcgtggtcc gcacgttcgg tcaggccgat
    12661 ttccagttca gcaccctggg tggaagcacg accaatccga actcggtcaa ccagactgcc
    12721 ccggtcggcg gcgttcagct gcttgacacc cccggcggcg gttacgtcgc ggtggagtac
    12781 cttttcatcc agttcgccgg cttcactttc ggcaagtcgt cctcggccta ttcgacgccc
    12841 tggaacggtt tcccgggtaa catcaattcg agcttgctcg gcggcaacaa taccgatacc
    12901 ggcgtcaaca acatccagta caccgctcag ttcggcaatg gcgtgtccgc caccatcggt
    12961 cttgatgatc cgaccgtttg ggaccgtacc atcgtcgcca atctctcgct cccgttgagc
    13021 gcgaccgagg gtgcggccaa cgcttatgcc ggcacgcatg cccccgacgt cgtcggcaat
    13081 atccgcattg atcaggcctg gggtctgttc cagatttcgg ccgcggcgca tgaggtcagc
    13141 ggttcgtata acgtcctcaa cacggcggcg gttcctgcgg gggccaccgg tcttgccggc
    13201 gcggccccga ccgccctgtc ggaaatcagc ggccaccccg aaaccaaatg gggcggttcg
    13261 gtgatggctg ccttgaacat caagaacatc ccgacaggtg ccggcgacga catcaagatc
    13321 gacgcgagct atgccaaggg cgacaccaag aacgtgattg ccaccagcgg aaactcgccg
    13381 agcttcgcga tgtttggcgg cagcaacacg gcctaccaga gcgtcgggtt tggtcttaca
    13441 tccgatgccg tctatcttcc ggtggcgaac ggtggcgacg gtaggctgca tctgaccgac
    13501 gcgtacggcg tccgcggtgc gttcaaccac aactgggatc cgtactggtc ttccagcctg
    13561 tttggcagct actccgcggt tcgctacgac ggcacggcaa aggccaacat ctgcacgaac
    13621 tacacgaccg cgggcaaggc agttggtggc gtgatcgcct tcacctgcaa cccggactac
    13681 aacgtctcgc agctcggttt tcttacccgc tggaccccag tcaagaacct gacgttctcg
    13741 gccgaagttc agtggttcca cctcgaccag aaattctctg gtgtcgcgac cttgggcccg
    13801 acggctccca agccctcggc cgtctacgag ttcaaggacc aggatacgtt cttgctgcag
    13861 gttcgcgcgc agcgtaactt ctgatcgcgg cgaccttacg gttgtggggt gatccggaaa
    13921 cggcatcgag gcgcggggcg agaagattat ctcgcgtcgc gcctcggtgc cgttttcggc
    13981 tccgtatcaa taccgacgga agaatggcgc ggtctgcggg cccgttcgtc aaccctttgg
    14041 taaccaatcc actctagttt ctgccgggct cgctggagaa atctgcgaaa cgcaggtagg
    14101 agttttgaat gcggcatgtg ttttcaaaat tcctccgtga ccagtccggc gcgaccgcca
    14161 tcgaatattg tctgatcgcg gttggcatcg ccatcgtgat catcgccgcc gtcaatggca
    14221 tcggcacgac cctcaacacc ggtttcgcct cggtgaattc gtcgctgaaa tagcggcggg
    14281 ttgggcagcg cgcgccatgc aggagctgct attcgggacc tggagcccgc aagtcatagc
    14341 ctacagccag caatatccct ggcgcgtatt cgccgccgtg ctggtcgcga tcccgttgtt
    14401 ggattttatg ttcctaaagg gccgattgtc cggcctgggc gacgggggcg atttcggcgg
    14461 atccggcgga tctgattgtg gcgatggcga cggaggcggt tgcggcgatt gagatcgcgc
    14521 tcaccgtcct tttccatccc cattcagcgc ccgcattacc ccgatccggg caaacatcag
    14581 ccagccgccg ccggtctcgg cagcattgat caggttctcg accgcaattt gccagagctc
    14641 ttgctgctgc acatcttccg gcagcttcat gatgtggtcg gcggcgtgct gcagggtcgt
    14701 gagcgtgcca cccccgtgca ggcgtatcgg cggatcgaac ggcgtcgacc acggcatctc
    14761 aggccgcgtg atgcgctggt cgcgaagtca cgtggtatct gcccgaaatc ttcgggcgcc
    14821 gccgcgaagg ctcgcgcatt catcccgcct ttttcgaccg ggcgggcgcg cgcaccggct
    14881 tctccgcccg cttcggctct tgcgccttcg cttcttcctt ggcggtgcgc ttgccgctga
    14941 tcggcagcag catctcgcgc tggcccgaag cggccttgcg cggcttcttg ccttttgcag
    15001 ctacgggagg ggcggattgt ttttcgctgg ccaggctgcg cttcagcgcg tccatcaaat
    15061 tgatgacgtt accgcttgat ttcggtgctg ctttcgctgt cgtgcgaatg ccgttgcgct
    15121 tctgattgat gagatcgacc agcgccgatt cgtagcgatc ctcgaaccgg tcgggttcga
    15181 acgatccgga tttcttttcg acgatatgcc tggccagatc cagcatatcc ttggtgacct
    15241 tcacgtcctg gatgtcgtcg aaatattcct tctcgctgcg cacttcgtaa gggtagcgca
    15301 acagcgtgcc catcaggccc ttctcgagcg gctccagcga gatgatgtgc tcgcggttgg
    15361 tcagcaccac gcgcccgatc gcgaccttgt tcatgctgcg gatggtttcg cggatgaccg
    15421 cgaaagcgtc atggccaacc ttgccatcgg gcacgagata atacgggcgg atcagatagc
    15481 gattgtcgat atcggttttg ggcacgaact cgtcgatctc gatggtgcgc gtcgactcca
    15541 gcgcgatgtc gtcgagttcg tcctttgaca cctcgatata ggtatcggtg tcgaccttgt
    15601 agcccttcat gatgtcgtcg gcggcgactt cctcgccggt gtcggcatcg accttggcgt
    15661 atttgatgcg atggcccgtc ttgcggttga tctggttgaa gctgactttc tcggtatccg
    15721 acgtagccgg atacagggcg accgggcagg tcaccagcga aaggcgcaaa aagcctttcc
    15781 agttcgcacg gggggccatg ggatactccg aatacgccgc agacgaccgg ccacgatacc
    15841 accggggaac ccggattcaa ggcgagcggg aagggaatct tccattggcg ttaaccgggg
    15901 cggggccggt tggttgcaag aggaccccct ccgcactggc ttcgcccgcg agccgcgagc
    15961 tgacatcggc gcaaaaatcc ggaacatcgt tttcgatcat gcgtttgctt caacacgcgc
    16021 gccgccaggc gttcgtgaca tcagtgaatc gagggcacca tggcaacgcg accccagcgc
    16081 cagatcgtcg aaaccccgac cgaagcacgc caggccgaac cgggtccgtc ggtgcttgcc
    16141 ctcctgaccg tctcgaccgg gcttgcggta ttgatcctcg gcgccatctg gttcgcgttc
    16201 ttccgcatct gagcggtacc cgcgtcggct acagccaggc agtttgaatg gcaaaaaggg
    16261 gcccggcagc cagccgttcg gccaccgggc agtctctgag gaaacgcccg acatgggctc
    16321 cgtacggcga cgcgacgaac aatggtcgcc caatgctcgc cctctgcgac cgagccagag
    16381 ggggcccgca tcgcgtcgcc gaatttcaat gcgccggaag ccatcccatc cagatgccga
    16441 agagcatcgc agacgacggc ggagacgaga acggcaaaca ccagcgtcca gcagctaaag
    16501 tcgagataga gtttcatcgg atgaggttcg ccggcaaacg gcctcaagcg cgacccgcac
    16561 ggaagaggcg gagccgagag cgaaggcctc acgcatcccc gcggacgatc cttctttcct
    16621 cctcgtcacc cgttcggtcg tttcgttgcc gcacttcgcg cactgataga agatccgcaa
    16681 actgggactg atcagctaca acggaaaaat tgaagtccac aattcgctcc tgagcgccgt
    16741 gttgcacgac aagcccgagt taaagcggga aaccgatctt gatgaaacca tccttgacga
    16801 agccagcttc gccaatgctg tcccggaatc agctcgcctt gccctcggga ttttgaaccg
    16861 gcgtgccgat cagcggtgcc ttgtcgatgg catcgcgcaa ttgaatcgcg gcggcggcgg
    16921 tgcaacgcaa atgggccacc gcgacgggaa tgacatcggt gccaggtccc cccgacggca
    16981 tgataatgcc ggcggcgagc acgatgttga tcaggccgtt gtggtttccg aacgtcgggc
    17041 agaattcgaa gaaaacaatg ggagcggagg gtagatcccg gaccgggata aattggaatt
    17101 taaattataa attggaattt accctgacgg cgcgcatccg aacctcttcg cgatcctgcg
    17161 ttgctttcgg cactcgcgtc gtcaggcgtt cgtgacagca acgaatcgag ggcaccgggg
    17221 caacgccact gctgagggat ggaacgcgtg gcgcgcgggg gaagtccccc gtttcgatag
    17281 aatcgaaacg aagctccagg cgacctgttg gagctttcac gtcggcatgt taggcgcttg
    17341 gatcagcggc cggccgctcg aaaggccggc accggccggc cttggacgcg accggaaaat
    17401 ttggcaaaag cggttgtgaa gaaaaagtaa tattcggtgt tattcttgtt catgggccgt
    17461 tcacgcccgc gaggctcatg ttgaggtggc agttatgcca ttcatttgcg gagattacct
    17521 tgcgcctgct ggttgttgaa gacgatcccg atctcaatcg tcagctcacg acggcgctga
    17581 ccgacgccgg ctatgtggtc gatcgcgcct tcgacggcga ggaggggcat tatctcggcg
    17641 acagcgagcc ttacgatgcc gtcgtgctcg acatcggcct gccgaagatg gacggcatct
    17701 cggtgctgga ggcctggcgg cgcaacggac gcgcgatgcc ggttttgatc ctcaccgccc
    17761 gcgatcgctg gagcgacaag gtgcagggct tcgacgccgg cgccgacgac tacgtcgcaa
    17821 agccgtttca tctcgaggaa atcctggcgc ggatccgggc gctgctgcgc cgctccaccg
    17881 gtcacgccca gtccgagctg acctgcggcc cggtgtcgct ggatacccgc accggccggg
    17941 tcagcgtgtc aggcaatccg atcaagatga cgtcgcacga gtaccggctg ttggcctatc
    18001 tcatgcacca caccgggcgc gtggtgtcgc gcaccgaact cgtcgaacat ctctacgatc
    18061 aggatttcga ccgggactcc aataccattg aagtgttcgt cggccgcatc cgcaagaagc
    18121 tggatgtcga tattatccag accgtgcggg ggctgggcta cctgctgacg ccgccgtcgc
    18181 ctcgggcttg attaaaggtg ttgatcaggc gccagactta acgttttgtt tgagcatgat
    18241 tttgaccgaa aaccggcatc gacgtttcgg gatcatgcgc gatgcgcggt agctcgctcg
    18301 ccacccggct gttcttgtct gcgaccgcct gggtggtggt gatcctgctc atcaccggag
    18361 tggtgctgtc gtcggtgtac cgcgacgcga ccgaacgcgc cttcgaccgt cgtctcaacc
    18421 tctacctccg gaccctgatc gccgaagtcg cgaccccgga cgagccgccc gaccatcagt
    18481 tccagtcgct cggcgagccg ctgttcgaac tgccgctgtc cggctggtat tggcagatca
    18541 cccgcaccga ctccgagaag gccgaggtgc gcgcgtcgcg ctcgctgtgg gacaagaagc
    18601 tgccgaaact tgaagaccac ggcgccgact tgaccgcggc cggcatccgt cttggctatg
    18661 tcgacggccc cgagggccag aatctgcgga tggtggaacg gccggtcgat ctcggcgccg
    18721 atggcaagtt tctggtcagc gtggccggcg acgccagcga gattttcgac gaaacccgca
    18781 gcttcgatta ttacctcggc ggcaccttcg tcgcgctgtc gatcgtgctg gtgctgacaa
    18841 cgatcttcca ggtccgtttc gggctcgcgc cgctgaaacg catctcggaa tcgatcgccg
    18901 atatccgctc aggccgcgcc gaacggctcg aaggcgaatt ccccgtcgag atcgcgccgc
    18961 tggcgcgcga gaccaacgcg ctgatcgacg ccaaccgcga gatcgtcgaa cgcgcacgca
    19021 cccatgtcgg caatctcgcc cacgccatca agacgccgct gtcggtgatc gtcaatgagg
    19081 cttccgcgca tggtgccgat ccgttcgcga ccaaggttct ggagcaggcc gatgtgatgc
    19141 gcgatcaggt cgcgcaccat ctggagcgcg cgcgcatcgc cgcgcgtctc accattatcg
    19201 gcaccgtgac cgaggtcgcg cccgcgatcg aggcattgcg ccggaccatg gagaagatcc
    19261 atcgcggccg cggcatcgcc atcgcggtcg aggccgaccc gcaggcgaag tttcgcggcg
    19321 agcgccagga cctcgaggag atggccggca atctcgtcga caatgcctgc aaatgggcgg
    19381 cgtcgcaggt cttcatcgag gtgctggtgg agcggccggt caaggccggc gccggtccga
    19441 tgctgcgcat cctcgttgac gacgacgggc gcgggctgtc ggcggccgag cgcgcgcagg
    19501 tctcgcgccg cggccagcgg ctggacgagt ccaagccggg ttccgggctc ggattgtcga
    19561 tcgtggtcga tctggcggcc ttgtacggcg gcagccttag cttgggcagc gcaccgatcg
    19621 gcgggttgcg ggcggaactg gtgttgccgg gggtatgagt accgtttgcg gcagaccccg
    19681 gcgaccggct ggaacagccg ctcgtaaccc cgatttcatc attcctaaac gacttcttaa
    19741 cgccggccct tttattatga ccgccggacg ggtttcaccg tccgcgtcaa accaatgtgc
    19801 ataccgggcg catgagccag acatcgatag agcggctgag ggactatctc gcgcaacttc
    19861 cgccgcaggc gcaggccttg ctgatgcggg agttcgagcg cgccatcgaa cgcggcgagg
    19921 atgccacggt cgccaccctt gtgctcgaac agttgcgcaa ggtcgtgcgc ggaaccgacg
    19981 aggagacgcg ggcgcgcacc gacgatccgg cgcggctact gttccgcccg ctcgaaccgt
    20041 ttctcatcga cagcaacttt ccggcacggc cgggtcagat tcgacgcgct tcgttgccgc
    20101 cggtgtggca atggctgatc cgcgacggcg cgccggacca ggcacgcgag ttcgaagcgg
    20161 cgctgacctt ggtgcgggaa accggaacga catccggtct cgaggcggcg atccgcaaat
    20221 tccagctcgc ggcggcggac gcgatctaca agattgccat gccggtgccc ggcgaggaca
    20281 agcagcgcgc gctgtcccgc gtcggtccgc ccaatgtgat cgaggatctg ctgccgatcg
    20341 gctcggtttt gcaggcccgc gaagcgctgg acggcctcaa cggccggctt cccagcaact
    20401 tgcgaatact tgcggacgcg cagatcgcga cagtcagcgc cgcgctcaac gttcccgccc
    20461 tgcaaacgcc gcaaacgctg ccgtttgcgc tgtcgctgat cattcagcgg ctgaccgcgc
    20521 cgtggcaaat catccgcctc gcgatcaaga tggccgcgtc cgacgacgag atccgcgttg
    20581 cggccactcc ctacggcatg gccgtgacca tagccctgca cgatctgtac ttcctcgcgg
    20641 cctgtctgcg aacggacatc aagcgcggcc atttcgacga cgtcgccgag cagctaaaaa
    20701 gcctgcatga cggcgtgcgc ggcctgcgga ccgaactcga tctccgcaac gattcgtcat
    20761 ggggcaagca actgacgtcg atccgggccg atatttccaa caccctgcaa tcggaaatcg
    20821 acagcgtgcc cggccgcgtc cggcgtttgc tgcgtcagcg cgccgacaag gacatttccg
    20881 tcggcgccaa gatcgatact tccgaagtcg acgaaaccgc cgcattgatc gatttcgtcg
    20941 cggtgtgccg tacctatgcc agcgaactgg cgatcaacga ggtcacgctg cgcacctatt
    21001 ccgacctgca acaatatgtc gaaaagtcca ccgaggcgtt ggtgcagtcg ctgcgcggcg
    21061 gcgacgccaa ggcgcgcgcc taccggcaga tgcaggtcaa ggcggcggtg cggttttgcg
    21121 aagtattgtt cggccacgac tatgcatcgc tgatgagcag ggcggcggaa aatgcgctga
    21181 ccggcgagcg caagccttcg cgcgcgggat aaaagggaca agagatagta gaaaactcca
    21241 tccacaattt tcggttgaaa cggatcatgc gcgcgacgta cagcttttga cgtacagctt
    21301 ttagtgcaaa ctcaatcatc aattccgctg aatttgagat atgaactcaa tcatttattt
    21361 cgttgaactt gaaaatcgcg tgatctcggc gacataccgg aacctgatga tcggagcgaa
    21421 ggtcgtgatc gtcgacaaga ccagcggggc gcaattgtcc gatccggtca ccacgatcgc
    21481 atctccggtc cccaacggct cgctgcggat cagacttccc gaggcggtca aaccgggggc
    21541 ctattacttg aaagcgttga acgggcatgg cgagcaggcc gcgcaaagtg tcgagtttca
    21601 tgtcggctaa ccccccattt tgactgggtt tcgggattta tcggccgcca tggcgaatag
    21661 agaattgttg gttgcccggc ttggcgccgg tggtgtaaag cggcgcattg gtgcggttgg
    21721 cgccgttttg tcggaacctg ctcgatgacg ctgtggtttg tgttcgcgct gatgacggct
    21781 gcggcgatct tcgccgtgct ctggccattg ggtcgcggta cgaggccgca aaacgacggc
    21841 agcgagaccg tcgtctacaa ggatcaactc gccgagatcg aacgcgacgt ttccgcaggg
    21901 ctgattggcc gctccgaagc ggacgccgcg cgcgtcgaga tcagccgccg gctgctggcc
    21961 gcggccgatc atcagcgcgc gccggccgtc gtatcgagta tcaagttgcg ccgctcggcg
    22021 gccatcacgg ccctggttgg agttcccatc gtggcggttg cgttttatct tccgctcggc
    22081 tcgccgcagc ttggcgattt tccgctggca caacgcaccc atgcgcccga cgtcacacag
    22141 ccgcttgaca atctggtggc acaggtcgag cagcacctgg cgaaaaatcc aaccgatggc
    22201 cgtggctgga gcgtgcttgc gccggtgctg gcaaggttgg gccgctacga cgacgcggtc
    22261 cgtgcctacc acaattcgat cacctataac ggcgacagtg cgacgcgccg ggctgatctc
    22321 ggcgaggcgc ttgccgcagc ggccggtggc gtcgtcacgt cggaggccaa ggctgaattc
    22381 gaacgcgcgc tcgcgctgaa cccggacgag gtcaaggcgc ggtacttcct tgggctcgcc
    22441 gccgagcagg acggccgcac cgcggaggcc aattcgatct ggggggcgat gctcgcaaag
    22501 gcgccgcctg atgcgccgtg gcgtccgctg gttcaagcgg cattggcgcg ggtcggcggc
    22561 accaccgctc cggcgctgtc ggatgaggcg gttgccgcgg caagggacat gaacgaggcc
    22621 gaccgcggca cgatgatccg tggcatggtc gagcgtctcg ccagtcgatt gaagcaaaac
    22681 ggcgacgacg tcgacggatg gctgcggctg gtgcgggcct atgtggtcat gggcgatcgc
    22741 gacaaggcaa ggggcgcgct caccgacgcg cgtcaggccg ttgccaacga tgcggaacgg
    22801 ctgcgacaac tgaatgaggg cctgaagaat ctcgggctcg atggatgagc aggattgggg
    22861 gcgcatgatg ccgaaaagtg tgaagcgatt ttcggatcac atcatgcgca ggattagagg
    22921 ccacgcatga cgcgcaagca gcggcgtttg acaatgatcg gcggctcgct tgcggttcta
    22981 gcggtcgcag cagcccttgt gctgaatgcg atgcgcgatt cgatcgtgtt tttctcgacg
    23041 ccggcgatgg tggccgagaa acatgttccc gcaggcaagc gtttccggct gggcggcttg
    23101 gtgcagccgg ggtcgctgct gcgtggcgac aatttggcgg ttgcgttcag ggttgccgac
    23161 ggcagcgccg cattgcccgt ttcgtacaag ggaattctcc ccgatctgtt tcgggaaggg
    23221 cagggcgtgg tcgctgaagg cgcactcgat tcctcaggcg tattcgaggc ggataccgtg
    23281 ctcgccaagc atgacgagac ctacatgccc aaggatgttg cggacgcgct gaagaagcag
    23341 gggcactgga gagacgatta cggcgccaaa ccggattatg gcgcaaaatc cgcgattacc
    23401 cagggggcag cgcgtgcagc tcaaagcgcg ccgcaggccg atcgacctgc gccggagccg
    23461 aactcgggag cgttgcagtg atcgcggaaa caggacatta cgcgctggtg ctggcgttgg
    23521 ccctggcgct gatccagtcc acgctgccaa tcctcggtgc gcgctggcac gatcctgccc
    23581 tgatgaacgt cgcgcgctcg accgcgctgg cgcaactgct gttcgtggcg gcgtcgttcg
    23641 ccgcgctggt tacgttgcac gtcacatccg atttttcggt cgtcaacgtg ttcgaaaatt
    23701 cgcattcctt gaagccgctg atctacaaga tcaccggcgt gtggggcaac catgagggtt
    23761 cgatgctgct gtgggtgtcg atcctggcac tgttcggcgg cctcgtcgcg gcgttcggca
    23821 acaatttgcc gctgtcgctg cgtgcccacg tgctggcggt gcaggcctgg attgccagcg
    23881 ccttctatct gttcatcctg atcacttcga acccgttcct gcgcatcgac aatccaccga
    23941 tcgagggccg cgacctcaat ccggtgctgc aggatatcgg cctcgcggtg catccgccga
    24001 tgctctatct cggctatgtc ggattctcga tctcgttttc gttcgccgtc gcggcgctgc
    24061 tggagggccg gatcgacgcg gcgtgggcgc gttgggtgcg gccgtggacg ctggtggcgt
    24121 ggatattcct tacgctcggc atcgcgatgg gctcgtactg ggcctattac gaactcggct
    24181 ggggcggctg gtggttctgg gatccagtcg agaatgcctc gctgatgccg tggctcgccg
    24241 gtaccgcgct gctgcattcg gcggtcgtga tggaaaagcg caacgcgctc aaggtctgga
    24301 ccatcctgct tgcgatcctg actttctcgc tgtcgttgct cggcaccttc ctggtgcgct
    24361 cgggcgtgct gacttcggtg catgcgttcg cgacggatcc gacgcgcggc gtgttcatcc
    24421 tgctgatcct ctgcattttc atcggcggca gcttgacact gtacgcgtgg cgggcctcgg
    24481 cgctgaagca gggcgggctg ttcgcgccga tctcgcggga aggcgcgctg gtgttcaaca
    24541 atctgttcct taccactgct tgcgcgacgg tgtttatcgg aaccttgtat ccgctggcgc
    24601 tggaagtcct gaccggcgac aagatttcgg tcggcgcgcc gttctttaat ctcacctttg
    24661 cgccgctgtt ccttcccttg atgatcgcgg tgccgttcgg gccgttgctg gcatggaagc
    24721 gcggcgacct cttgggggcg gcacagcgcc tgatcgccgc cggcatcgcg gcgctggttg
    24781 ccattgcggt gctatgggca tggacccgcg gcggcagcgc cttcgcgccg ctggcgatcg
    24841 ggcttgccgt cttcgttatc gcgggcgcgc tttgcgacct tgccgagcgc atcggcctgt
    24901 ttcgtatgcc attcgcaacc gtcctgcggc gcgcgcgcgg attgccgcgt tcgacctggg
    24961 gcacgacgtt cgcccatgcc ggcgtcggcg tcgcgctgat cggaattgtc tgcgagacca
    25021 cgtggaacag cgaatttatc ggatcgatga agcccgatga cgtcgcgaaa atcgccggct
    25081 acgaattgaa actcgacggc ctgacgcagc ggcaggggcc gaattttcgt gaaatgctgg
    25141 cgcagttcac cgttacgctc gatggcaaac tgctcagcgc gatgacgccg tcgaagcgta
    25201 attttcccgc gcggggatca tcgaccaccg aggcggcgct gctgacgcgc ggcgcaagcc
    25261 agctctatat ctcgctcggc gataccacgg ccgatggcgc catcgcggtg cgcatctatc
    25321 ataagccgct ggtgctgctg atctggtggg gacctgtgct gatggcgttc ggcggcgtgc
    25381 tgtcgctgtc ggatcggcgg ctgcgggtcg gcgcgccgaa acccgccaaa gttctgcgcg
    25441 gcctgcaggc ggcggagtga tcgaatgaaa ccgatccttg cctgcgtctt tgccatggtc
    25501 gtgatgatgg gatcgtcggc cgcctatgcc gtgcagcctg acgagatcat gtcggatccg
    25561 gcaatggaaa cgcgggcgcg cgatctgtcg cgcgaattgc gctgcatggt gtgccagaac
    25621 cagtcgatcg acgattccga agcgccgctg gcgcgcgatt tgcggctgct ggtgcgcgag
    25681 cggatcgcgg ccggcgacag cgatggccag gtgatcgatt tcctggtggc gcgctatggc
    25741 gagttcgtgc tgctgaaacc gcggctcaag ccgcacacct tgttgctgtg gctgttgccg
    25801 ccgctggcgc tggccggcgg cggatttgta ctgtggacgc acggccggcg gcgctccaga
    25861 tccgcggcag cggaagatca atcgctgctc cgattgaccg cggaagaaga agcgcggctg
    25921 gaacggctga ccgcggcgga accggcggcc gacaagccgg cccagggttc gcgataagcc
    25981 gtttttgccc ggcgatcggc cctttctcac cccctgtgat aaatcgtttt cgtcctgcga
    26041 taaactgccg cattgccgtc ctgcttcatt accaaagttt aattccctag ccagcgcgcg
    26101 gtaaggcgga agaccccatc ttggggcctg taaggtgctt gccccgcgca acctcgattt
    26161 cacgctctca cgctcacgct ctggagacct tttaaatgac cgaccgtccc accgatcttt
    26221 cgtcactacc gtcttaccag acaccacgcc gttcgctgtt ctccgcccgc aaattcgcgc
    26281 tgatggcctc ggttgttgcc gggctcggcg tcgcggttta tggcttcagc ccgtcgtccc
    26341 gcccggtaga tgttttctcg agccccgcgc acgcgcaagt caacaacgaa gttcgtaaag
    26401 tagaaaggcc cgtcggcttt gccgacattg tcgagcgcgt gaaaccgtcg gtgatctcgg
    26461 tgaaggtcaa tatcaacgag aagatcgcca aggacgatag caccaacaac aatgatgact
    26521 cgccgttcca gccgggctcg ccaatggagc gtttcttccg ccgcttcggc ggacccgatg
    26581 gcatgccgcc gggcatgcgc ggtggtccgc gcggccgagg tgccgtgacc ggtcagggct
    26641 ccggcttctt catttcaccc gacggctatg ctgtcaccaa caatcacgtg gtcgacggcg
    26701 ccgacaaggt cgaagtcacc accgacgacg gcaagaccta tactgcgaaa gtgatcggca
    26761 ccgatgcgcg caccgacgtc gcaccgatca aggtcgaggg
//
LOCUS       DATNOI010000641         9488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_890166,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000641 DATNOI010000000
VERSION     DATNOI010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9488
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890166"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..727
                     /gene="paaI"
                     /locus_tag="VN968_25935"
     CDS             263..727
                     /gene="paaI"
                     /locus_tag="VN968_25935"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HXL32706.1"
                     /translation="MNVKARLSPDDLARACADAMWQDDDASKGLGMEIRRIKPGEATL
                     TMTVQPHMVNGQGIAHGGFIFLLADSAFAFACNSHNERAVAAQCNISFIRPGKLGDLL
                     IATAREISRTGRSGIYDVRVTVDDTPIAELRGHSRTVGGAWVPLVEPDARLK"
     gene            766..2097
                     /gene="paaK"
                     /locus_tag="VN968_25940"
     CDS             766..2097
                     /gene="paaK"
                     /locus_tag="VN968_25940"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HXL32707.1"
                     /translation="MASTNPQSTSSDYRAEMDQTERASRDEIIALQTRRMAWTLAHAY
                     DNVAHYNNAFDKSGVTPQDFRQLSDLSKFPFTAKADLRQNYPFGMFAVPREKLVRLHA
                     SSGTTGKPVVVGYTRADIDTWSDVMARSIRAAGGRTGMILHNAYGYGLFTGGLGVHYG
                     AERLGCTVVPVSGGMTERQVQLINDFRPDIITVTPSYMLAISDEFKRQGLDPRKSSLK
                     FGIFGAEPWTNAMRAEIEAAFEMDATDIYGLSEVIGPGVAQECVESKDGLHVWEDHFY
                     PEVIDPGTGAVLPDGEKGELVFTSLTKEAFPVIRYRTRDLTRLLPGTARPGMRRMEKV
                     TGRSDDLIILRGVNVFPTQIEEALLATDWCGGHFIIELTREGRMDEMTVLAEARPERW
                     DSSGLIEHTERVAAHIKNTIGISARIRAVAPETLERSLGKAKRVFDKRPKE"
     gene            2227..3657
                     /gene="iaaH"
                     /locus_tag="VN968_25945"
     CDS             2227..3657
                     /gene="iaaH"
                     /locus_tag="VN968_25945"
                     /inference="COORDINATES: protein motif:HMM:NF005688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indoleacetamide hydrolase"
                     /protein_id="HXL32708.1"
                     /translation="MADSSSSTSAEIVADIRTGKMTAVSAVQAALERAEKFKNLSAFI
                     ILNRDGALAAAAQVDAGEKRGALAGLPIVVKDNINTADMPTSGGTPALRHGRPTKNAP
                     SLQKLLDAGAIIIGKTNMHELAFGITSTNLSSFAGPVKNPYDTTRIPGGSSGGTSAAI
                     AARIVTCGLGSDTGGSTRVPAALTGTVGLRPSVGNGGAQRRYDDANLVVPISHTRDTV
                     GPMGRTVADVALLDSVITGTAMAQAEPLRGKRLGIPASFWNGLDCDVEAVAKAARAKL
                     ADAGVVLVETDMAGLFEQNGKVSFVVALHEPIADIPAYLKASGIEGITLADIAGQIAS
                     PDVAGAFGAITADAFGAAYPDAINVQRPALQKIYEAYFRDNHLDAILFPTTIAPAPPI
                     DAEKGSSEMSINGGNPVPTFDTMIRNTDPGSNAGLPGLSLFAGMTPGGLPVGLEIDGP
                     VGSDEKLLGLGLSIELLLGSAPPPKG"
     gene            complement(3783..4865)
                     /gene="paaE"
                     /locus_tag="VN968_25950"
     CDS             complement(3783..4865)
                     /gene="paaE"
                     /locus_tag="VN968_25950"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HXL32709.1"
                     /translation="MSSSIPRFHRLAVNDLRRESPDAVSLTFAIPQALADDYRFAPGQ
                     YLTLRTTMDGAEVRRSYSICSGPDDGELRIAVKKVDGGAFSSWAADELKQGDELDVMT
                     PTGRFGVAPAPGEARLYVGFAAGSGITPILSIVKGVLAREPLGRFFLFYGNRSTGGML
                     FREALEELKDRFMQRLSLFHVISGEEQDIPILHGRLDGEKVRVLLRSLVPAASVDHVF
                     ICGPTGMSEDIEATCRDIGIAAERIHVERFVSEFGGKPRAKAIIPASAPPKALASLII
                     DGKRREVPVAEGEAILDAALRAGMDLPFACKGGMCSTCRAKLVEGEAQMEVNYSLEPW
                     ELSAGFILTCQARPTTEKVVVDYDHV"
     gene            complement(4868..5371)
                     /gene="paaD"
                     /locus_tag="VN968_25955"
     CDS             complement(4868..5371)
                     /gene="paaD"
                     /locus_tag="VN968_25955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HXL32710.1"
                     /translation="MVTAVFGDAGLRQRAWDTAAKVVDPEIPVLTIADLGVLRDVAVN
                     DGCVEVVITPTYSGCPAMNMITLEIELALAREGFRNPKVRTVLSPAWTTDWMSQDGRR
                     KLTAYGIAPPQAAGSRRALFGVQQVACPQCGSEDTEVLSEFGSTSCKALWRCKSCREP
                     FDYFKCH"
     gene            complement(5365..6141)
                     /gene="paaC"
                     /locus_tag="VN968_25960"
     CDS             complement(5365..6141)
                     /gene="paaC"
                     /locus_tag="VN968_25960"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HXL32711.1"
                     /translation="MASASIQVLETPLVLYVLRRADDALILGHRLSEWCGHAPMLEED
                     MALANMGLDLLGQARELYCYAADVEGKDNDEDKFAYLRDVRQYRNLLLLEQPNGDFAH
                     TMVRQFLYAGFADLYWRAMMTSGDATLAAIAAKSEKESAYHLRHSSEWIVRLGDGTEE
                     SHARAQTAIDDLWAFTGEMFEVDDSERGVIDAGIAVDPKGLHPQWLKTVLSVVSDATL
                     ALPKSDWMQQGGRSGRHSEHLGHLLSELQSMQRTFQGVAW"
     gene            complement(6141..6428)
                     /gene="paaB"
                     /locus_tag="VN968_25965"
     CDS             complement(6141..6428)
                     /gene="paaB"
                     /locus_tag="VN968_25965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HXL32712.1"
                     /translation="MATPNTPLWEVFIRSRNGLAHKHVGSLHATDATLALQAARDVYT
                     RRGEGLSIWVVPSSAITASDPSEKGMMFGPAESKIYRHPTFYDVPDEVGHM"
     gene            complement(6447..7448)
                     /gene="paaA"
                     /locus_tag="VN968_25970"
     CDS             complement(6447..7448)
                     /gene="paaA"
                     /locus_tag="VN968_25970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HXL32713.1"
                     /translation="MYTQALNTSEPEGPPIKPGIEDAARAAQFQARIDADDRIEPNDW
                     MPAAYRKTLTRQISQHAHSEIVGMLPEGNWITRAPSLRRKAALLAKVQDECGHGLYLY
                     AAAETLGASREELVDLMLAGKAKYSSIFNYPTLTWADIGAIGWLVDGAAIMNQIPLCR
                     CSYGPYARAMIRVCKEESFHQRQGFEIMLTLSRGSEEQKAMAQNALDRWWWPVLMMFG
                     PPDKASQHSDTSTRWKIKRFSNDELRQKFVDATVPQAQYLGLTIPDPGMKQNAAGNWE
                     YSAIDWNEFNAVLAGNGPCNRERLAARRKAHDEGAWVREAARAYAEKRKQGLNAQAA"
     gene            complement(7528..8394)
                     /gene="paaX"
                     /locus_tag="VN968_25975"
     CDS             complement(7528..8394)
                     /gene="paaX"
                     /locus_tag="VN968_25975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HXL32714.1"
                     /translation="MAHPLARIIEQLKREPSRTGSIVITVFGDAIVPRGGSVWLGTLL
                     EFFGTIDIDSGVVRTAMSRLAADGWLERSKVGRNSFYRLVKKGRQTFDAATKHIYDPQ
                     PSDWTGRFELLLIGNGEDRDASREALKNAGFGSPLPGVWVAPSGVPVPDEAARAIRLE
                     VSAEDDSGRRLLSESWPLDRTADAYLKFMKTFEPLRGWIGRRQRLTDVDAFTARILLI
                     HYYRRVVLRDPLLPTALLPKDWPGRAARQLCGEIYRGLLAASEQWLDRNGTNEAGPLP
                     KAGEELARRFGG"
     gene            complement(8472..9242)
                     /locus_tag="VN968_25980"
     CDS             complement(8472..9242)
                     /locus_tag="VN968_25980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL32715.1"
                     /translation="MSAAAQMLRPPSKTLMFLEGRAIHEFGAFLGALPLLSLAPKGDG
                     HPVLVLPGLVASDASTRPLRHFLRNRGYAVSGWRQGRNLGLRDGVRDAMIDLVHELSD
                     TYGRKISLVGWSLGGLYARQLAKMMPNRVRSVITLGSPFAGNPKATNAWRVYEMASGR
                     RADEEDTRFGGALSQTPPVPTTAIFSRTDGICAWQGCVEKTSAMSESIEVESSHCGMG
                     HHPAAVYAVADRLAQPEGKWAPFDRSGWRSLVYPDPAR"
BASE COUNT         1746 a         3065 c         2963 g         1714 t
ORIGIN      
        1 catcgatttc tttgggggct ggtatcacgg atgttcacgc tttcttggcg gctttcgaac
       61 gcgatttgat gcctgacggc ctcgttgaca ccatggtcgc ttcgcatcat gactaacgga
      121 ctgatcgccg agttcaagag gcttgttcca acaagcccgg actgcgaact cagagaacct
      181 cctggggaga aagtcttggg gagaacgtct tggatccggt cctgatcgac aagcggccgc
      241 ggaaacgcgc ggggagacca cggtgaacgt caaggccaga ctttcgcccg acgatctcgc
      301 gcgcgcctgc gccgacgcga tgtggcagga tgacgacgcc agcaagggtc ttggaatgga
      361 gatccggcgg atcaagccgg gcgaagccac gctgacgatg acggtccagc cgcacatggt
      421 caacggccag ggcatcgccc atggcggctt catcttcctg ctggcggatt cggcgttcgc
      481 ctttgcctgc aactcccata acgaacgcgc ggttgccgcg cagtgcaaca tctccttcat
      541 ccggccgggc aagctcggcg acctcctgat cgcgaccgcg cgggaaatct cgcgcacagg
      601 gcgttccgga atttacgatg tgcgcgtcac cgtcgacgac accccgattg ccgagttgcg
      661 cggccattcc cgcaccgtcg gcggcgcctg ggtgccctta gttgaacccg atgccaggtt
      721 gaaatgaagg ctgcaatgaa cagctgattt caggagaaac gcgcgatggc ttcgaccaac
      781 cctcaatcca cgagcagcga ttaccgcgcc gagatggacc aaaccgagcg cgcatcgcgc
      841 gacgaaatca tcgcgctgca gaccaggcgc atggcctgga cgctggcgca cgcgtacgac
      901 aacgtcgctc attacaacaa cgcgttcgac aagtccggcg ttacgccgca ggattttcgc
      961 caactgtcgg atttgagtaa atttccgttc accgcgaaag ccgatctgcg ccaaaactat
     1021 ccgtttggca tgttcgccgt gccgcgcgaa aaactggtcc gccttcacgc ctcctccggc
     1081 accaccggca agccggtcgt ggtcggatat acccgggccg atatcgacac gtggtcggat
     1141 gtgatggcgc gctcgatccg cgccgccggc ggccgcaccg gcatgatcct gcataacgcc
     1201 tatggttatg gcctgttcac tggcggcctc ggcgtgcatt atggcgccga acggctcggt
     1261 tgcacggtgg tgccggtttc cggcggcatg accgaacggc aggtgcagct gatcaacgat
     1321 ttcaggcccg acatcatcac ggtgacgccg agctacatgc tggcgatatc ggacgaattc
     1381 aaaaggcagg gcctcgatcc gcgcaaatcg tccttgaaat tcggcatctt cggcgccgaa
     1441 ccctggacca atgcgatgcg cgccgagatc gaagcggcgt tcgagatgga cgccaccgat
     1501 atttacggcc tgtccgaagt gatcggtccc ggcgtcgcgc aggaatgcgt cgaaagcaaa
     1561 gacggcctgc atgtctggga ggatcatttc tatcctgaag tgatcgaccc cgggaccggt
     1621 gcagtgttgc ccgacggcga aaagggcgaa ctcgttttca cctcgctgac caaggaagcg
     1681 tttccggtga tccgctaccg cacccgcgac ttgacgcggc tgttgccggg cacggcgcgg
     1741 cccggcatgc ggcggatgga gaaggtcacc gggcgttccg atgacctgat catcctgcgc
     1801 ggcgtcaacg tgtttccgac ccagatcgag gaagcgctgc tcgccaccga ctggtgtggc
     1861 ggccatttca tcatcgaact gacgcgtgaa gggcggatgg acgagatgac ggtgctggcg
     1921 gaagcgcgcc ccgagcgctg ggatagcagc gggctgatcg agcataccga gcgggtcgcc
     1981 gcccatatca agaacaccat cggcatatcg gcgcgcatcc gcgctgtggc gccggagacg
     2041 ctggagcgct cgctcggcaa ggccaaacgc gtattcgaca aacggccgaa ggaataaaca
     2101 tcagccaccc caggtcggga ggaatccgtg accgacaagc cctcacgccg ccatatactg
     2161 acatcatcga tagccctcgc cgccggtgct gcggcgctcc cttccctcgc gttgatgagt
     2221 tcagcaatgg ccgattccag ttcctccaca tcagccgaaa tcgtcgcgga catccgcacc
     2281 gggaagatga cggccgtcag tgccgtgcag gccgcgctcg aacgcgccga gaaattcaag
     2341 aacctcagcg cattcatcat cctgaacaga gacggcgcgc tggcggcggc agcgcaggtc
     2401 gatgcgggcg agaagcgagg cgccctcgcc ggtctgccga tcgtggtcaa ggacaacatc
     2461 aacaccgccg atatgccgac atcgggcggc acgccggcgc tgcggcatgg gcggccgacc
     2521 aaaaatgcgc catcgctgca gaaattgctg gatgccggcg ccatcatcat cggcaagacc
     2581 aacatgcacg agctcgcatt cgggatcacc agcaccaacc tgtcgtcatt cgcgggacct
     2641 gtcaaaaatc cctatgacac gacccggatc cccggcggct catcgggtgg aacctcggcc
     2701 gccatcgcgg cgcgcatcgt tacttgcggc ctcggatccg ataccggtgg ctcgacccgg
     2761 gtgccggcgg cgttgaccgg taccgtgggc ttgcggccgt cggtcggcaa cggcggcgcg
     2821 caacgccgct acgacgatgc caatctggtg gttccgatca gtcacacccg cgacaccgtc
     2881 ggtccgatgg gccgcacggt cgcggatgtg gccttgctcg attccgtcat caccggcacg
     2941 gcgatggcgc aagcggagcc attgcgcggc aagcggctcg gcattcccgc ttcgttctgg
     3001 aacggactcg attgcgacgt cgaggcggtt gcgaaggccg cgcgcgcaaa gctcgcggac
     3061 gcgggcgtgg ttctggtcga gaccgacatg gccggactgt tcgaacagaa cggcaaggtg
     3121 tcgttcgtgg tcgcgctgca tgaaccgatt gcggatatcc ccgcttatct caaggcatcc
     3181 gggatcgaag gcatcacgct ggccgatatc gccggccaaa tcgccagccc cgatgttgcg
     3241 ggtgcgttcg gtgccattac cgcggacgcc ttcggcgccg cctatccgga tgccatcaac
     3301 gtccagcggc cggcgctgca gaagatctat gaggcgtatt tccgcgacaa tcatctcgac
     3361 gccatcctgt ttccgaccac gattgccccg gcgcccccaa tcgacgcgga gaagggatca
     3421 agcgagatgt cgatcaacgg cggcaatccg gtgccgacat tcgacaccat gatccgcaac
     3481 accgatcccg gcagcaatgc cggccttccc ggcctgtcgc tgttcgcagg catgacgccg
     3541 ggcggcctgc cggtcgggct ggagatcgac ggtcccgtcg gcagcgacga aaagctgctc
     3601 gggctcggac tttcgatcga gcttttgctg ggttcggcgc cgccgccgaa ggggtgatct
     3661 ttctcgcgtc attccggggc agcccgttag ggctgaaccc ggaatccaga gacgatcagc
     3721 gcgagattcc gggttcgcgc tgacgcgcgc cccggaatga cggtggttga acgatgcgcg
     3781 cgctaaacat ggtcgtaatc caccacgact ttttccgtgg tcggccgcgc ctggcaggtc
     3841 agaataaagc ccgcacttag ctcccacggc tccagcgaat agttcacttc catctgcgct
     3901 tcgccttcga ccagcttggc gcggcaggta ctgcacatgc cgcccttgca ggcgaacggc
     3961 agatccatgc cggcacgcaa cgccgcatca agaatggcct cgccttccgc gaccggcacc
     4021 tcgcggcgtt tgccgtcgat gatcagcgag gccagcgcct ttggcggcgc gcttgccggg
     4081 ataatcgctt tcgcgcgcgg cttgccgcca aactccgaaa caaaacgctc gacatgaata
     4141 cgctccgcgg caatgccgat atccctgcag gtcgcttcga tatcctcgct catgccggtc
     4201 gggccgcaga tgaagacatg atcgacgctg gccgccggga ccagcgagcg cagcagcacc
     4261 cgtaccttct cgccgtcgag ccggccgtgc aggatcggga tatcctgctc ttcgcccgag
     4321 atcacgtgga acagcgagag ccgctgcatg aagcggtctt tcaactcttc cagcgcctcg
     4381 cggaacagca tgccgccggt cgagcgattg ccgtaaaaca ggaagaagcg gccaagcggc
     4441 tcgcgcgcca gcacgccctt cacgatcgac aagatcggcg tgatgccgga gccggcggcg
     4501 aagccgacat agagccgcgc ttcgcccggc gctggtgcga cgccaaaacg gccggtcggt
     4561 gtcatcacgt cgagttcgtc gccttgtttc agttcgtcgg cggcccaact ggagaacgcg
     4621 ccgccatcga cctttttcac cgcgatgcgc aactcgccgt catcggggcc ggagcagatc
     4681 gaataggagc ggcgcacttc cgcgccgtcc attgtcgtgc gcaaggtcag atactgtccg
     4741 ggggcaaatc ggtagtcgtc cgcaagcgct tgcggaatcg caaaggtcag cgacaccgcg
     4801 tcgggcgatt cgcggcgcag atcgttgacg gcgaggcgat gaaagcgcgg tatcgatgac
     4861 gacatgttca atgacatttg aaatagtcga acggttcgcg gcagcttttg cagcgccaca
     4921 gcgccttgca ggaggtcgag ccgaattccg acagcacctc ggtgtcctcg gagccgcatt
     4981 gcgggcacgc cacctgctgc acgccgaaca gcgcgcggcg ggagccggcg gcctgcggcg
     5041 gcgcgatgcc gtacgctgtg agcttgcggc ggccgtcctg gctcatccag tcggtggtcc
     5101 aggccggcga cagcacggtg cggacttttg gattgcgaaa gccctcgcgc gccagcgcca
     5161 gctcgatttc cagtgtgatc atgttcatcg cggggcagcc ggaataggtc ggcgtgatca
     5221 cgacctcgac gcatccatca ttcaccgcga cgtcgcgaag cacgccgaga tcggcgatgg
     5281 tcaaaaccgg aatctcgggg tccaccactt tagccgcggt atcccacgcg cgttgccgca
     5341 gcccggcatc gccaaacacg gcggtcacca tgcgaccccc tgaaacgtcc gctgcatcga
     5401 ttgcaattcg ctgagcagat gcccgagatg ctcgctgtgc cggccactgc ggccgccctg
     5461 ctgcatccag tcgctcttgg gcagcgccaa tgtcgcgtcg cttacgacag acaaaaccgt
     5521 cttgagccat tgcggatgca ggcccttggg atcaaccgcg atgccggcgt cgatcacgcc
     5581 ccgctcgctg tcgtcaactt cgaacatctc gccggtaaaa gcccagagat cgtcgatggc
     5641 ggtctgggcg cgggcgtggc tttcctcggt gccgtcgccg agcctgacga tccattccga
     5701 cgaatgccgc aagtgatagg cgctttcctt ctcggatttc gccgcgatcg ccgccagcgt
     5761 cgcatcgccc gatgtcatca tcgcgcgcca atagaggtcg gcgaagccgg cgtaaagaaa
     5821 ctgccgcacc atggtgtgcg cgaaatcgcc gttcggctgt tccagcagca agaggttgcg
     5881 atactgcctg acgtcgcgca ggtacgcgaa tttgtcctcg tcgttgtcct tgccttcgac
     5941 atcggctgcg tagcaataaa gttcgcgcgc ctggccgagc aggtcgaggc ccatattggc
     6001 cagcgccatg tcctcttcga gcatcggggc gtggccacac cattccgaca gccgatgccc
     6061 caggatcagg gcgtcatcag cgcggcgcag cacgtacagc accagcgggg tttccaagac
     6121 ctggatcgag gcgcttgcca tcacatgtgt ccgacttcgt caggcacgtc gtagaacgtc
     6181 ggatggcggt agatcttgga ttccgccggc ccgaacatca tgcccttctc ggatggatcg
     6241 gaggcggtga tcgcgctgga tggtaccacc cagatcgata gcccctcgcc gcggcgggtg
     6301 tagacgtcgc gcgcggcctg cagcgccagc gtcgcgtcgg tcgcgtgcag cgagcccaca
     6361 tgcttgtgcg ccaagccatt gcggctgcga ataaagactt cccacagcgg cgtgttcggc
     6421 gtggccatca tgacctccct gaatttttac gcggcttgcg cgttaagtcc ttgcttgcgt
     6481 ttttcggcat acgcccgggc tgcctcgcgc acccaggcgc cctcgtcatg cgccttgcgc
     6541 cgcgccgcca ggcgttcgcg gttgcacggg ccgttgccgg cgagcacggc attgaattcg
     6601 ttccagtcga tcgcgctgta ttcccagttg cccgcggcgt tctgcttcat gccgggatcg
     6661 gggatcgtca agccgaggta ttgcgcctgc ggcacggtgg catcgacgaa cttttgccgc
     6721 agttcgtcat tggagaagcg ttttattttc catctggtcg aggtgtcgct gtgctggctg
     6781 gccttgtcgg gcggcccgaa catcatcagc accggccacc accagcgatc cagcgcgttc
     6841 tgcgccatcg ccttttgttc ctcggagccg cgcgacagcg tcagcatgat ctcaaagccc
     6901 tggcgctggt gaaaggattc ctccttgcag acgcggatca tggcgcgcgc atagggacca
     6961 taggaacagc ggcacagtgg aatctggttc atgatcgccg cgccgtcgac cagccagccg
     7021 atcgcgccga tgtccgccca ggtcagcgtc ggataattga agatcgagga gtacttggcc
     7081 ttgccggcca gcatcagatc gaccagttcc tcgcgcgagg cgccgagcgt ttcggccgcg
     7141 gcatagagat aaagcccgtg accgcactcg tcctgcacct tggcgagcag cgcggccttg
     7201 cggcgcagcg acggcgcacg cgtgatccag ttgccttccg gcagcatgcc gacgatctcg
     7261 gaatgcgcgt gctgagaaat ctgccgcgtc agcgtcttgc gataggccgc cggcatccag
     7321 tcgttcggct cgatgcgatc gtcggcatcg atgcgcgcct gaaactgcgc cgcgcgcgcg
     7381 gcgtcctcga tacctggctt gataggtggg ccttcaggtt ccgacgtgtt gagcgcctgg
     7441 gtgtacatgg ccgatctccg ggggaaggcg acccttaaca tataacaaaa tacatggaac
     7501 tcaatatatt tttgtaacat atcccgccta cccgccgaac cggcgcgcca gctcttcgcc
     7561 ggcctttggc agggggccgg cctcgttggt gccattgcgg tcaagccatt gttccgacgc
     7621 ggcaagcaac ccgcggtaaa tttcgccgca gagctggcgg gccgcccttc ccggccagtc
     7681 cttgggcaat aaagccgtcg gcagcagcgg atcgcgcagc actacccgcc ggtagtagtg
     7741 gatcaacaag atccgcgccg tgaacgcgtc gacatccgtg agccgttgcc gacggccgat
     7801 ccagccgcgc aacggctcga acgtcttcat gaatttcagg taggcgtcgg cggtgcgatc
     7861 gagcggccag ctctcgctga gcagtcggcg tccgctgtcg tcctcggcgg aaacttccag
     7921 acggatcgcg cgcgccgcct cgtcgggaac gggcacgcct gacggcgcga cccatacgcc
     7981 cggcagcggg ctgccaaaac cggcattttt cagcgcctcg cgggacgcgt cgcggtcctc
     8041 gccgttgccg atcaaaagaa gctcaaaccg tccggtccag tcggacggtt gcggatcgta
     8101 gatatgtttg gtcgcggcat caaaggtttg ccgccctttc ttcaccagcc ggtagaaact
     8161 gttgcggccg accttgctgc gctcgagcca gccgtcggcg gcgagccgcg acatcgcggt
     8221 gcgcaccacg ccgctgtcga tgtcgatggt tccgaagaat tcaagcagcg tgccgagcca
     8281 gaccgaaccg ccgcgcggca cgatcgcatc gccgaacacc gtgatcacga tcgagccggt
     8341 gcgcgagggt tcgcgcttga gctgttcgat gatgcgggca agcggatgcg ccatccgcca
     8401 cgcatagcgc gtttttcgga ttcacgacaa cgccgggccg cggcggtacg gtgtttacct
     8461 ggcgggatcg atcacctagc gggatcggga tagactaggc tgcgccagcc tgagcgatcg
     8521 aacggcgccc atttgccctc gggctgagcc agacgatccg ccaccgcgta gacggcggcg
     8581 ggatgatggc ccatgccgca atggctgctt tcgacctcga tgctttcgga catcgcggag
     8641 gtcttttcca cgcagccctg ccacgcgcag atgccatcgg tgcggctgaa gatcgcggtg
     8701 gtcggcaccg gcggcgtttg tgaaagcgcc cctccgaacc gtgtgtcctc ttcgtcagcg
     8761 cggcggccgc tcgccatctc ataaacgcgc caggcattgg tcgccttggg attgccggcg
     8821 aatggactgc cgagcgtaat caccgaacgc acccggttcg gcatcatctt cgccagttgg
     8881 cgcgcataga gcccgccaag gctccagccg accaggctga tcttgcgccc ataggtgtcg
     8941 ctgagttcat gcacgagatc gatcattgcg tcccgcacgc catcgcgcag gccgagattg
     9001 cggccctgcc gccagccgct gaccgcatag ccgcgattcc tgagaaagtg ccgcagcggg
     9061 cgggtcgagg cgtccgaggc gaccagcccg ggcaatacca gtaccggatg accgtcgcct
     9121 tttggcgcca ggctgagcag cggcaatgcg ccgagaaacg caccaaactc atggatggcc
     9181 cgcccctcca gaaacatcag ggttttggac ggcgggcgca gcatctgggc agcggcggac
     9241 attgtagctc ctttcagagc cggccccttg agcttcggca gccggctatt gaaccatagg
     9301 aagaaaacct catcaaatcg ttccgcgatg caacaatttc tagtcaaagc taggagcccg
     9361 tccagataag ttttgtgacg gacatttgtt gcagggtagc cggctcaagc ggcgattgcg
     9421 atggtgaaca gcttcaggag gttgtgggcg gtgcagatca tcgcccactc agcgcgcact
     9481 ttctcgac
//
LOCUS       DATNOI010000642         4392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1644805,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000642 DATNOI010000000
VERSION     DATNOI010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644805"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /gene="ugpC"
                     /locus_tag="VN968_25985"
     CDS             <1..1045
                     /gene="ugpC"
                     /locus_tag="VN968_25985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HXL32716.1"
                     /translation="HAVKDVNLQIRDKEFVVFVGPSGCGKTTTLRMIAGLEAISSGDI
                     SIDGNVINQLAPMDRDIAMVFQNYALYPHMSVYDNMAFGLKMRKFERPEIEKRVREAA
                     DILGIGELLKRKPRQLSGGQRQRVALGRAIVRHPRVFLFDEPLSNLDAKLRVQMRVEL
                     KKLHLRLGTTAIYVTHDQVEAMTLGDRVVVMKDGVVQQVGEPLELYNQPANKFVAGFI
                     GSPAMNFAAVTVTEANGSLMAENSGLRIKLPEETAQRLRGHVGHEVTLGVRPEDLTVA
                     GAADIDQPCFDAVIEVVEQLGSEILLDMKVGEGVMVASVEPTARVKVRDRLRVAMRPS
                     RLHVFDAKTEAAI"
     gene            complement(1177..4308)
                     /locus_tag="VN968_25990"
     CDS             complement(1177..4308)
                     /locus_tag="VN968_25990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HXL32717.1"
                     /translation="MPNKMLIDATHPEETRVVVVRGNRVEEFDFETAQRRQLRGNIYL
                     AKVTRVEPSLQAAFIEYGGNRHGFLAFSEIHPDYYQIPVADRQALIEADERAHREAEE
                     ESETRSNRRRPRHRNSRRRGNGERIQSEIVEHASTEPPEAAKSYEDQQRQEEGQQHPQ
                     EPAQNLHGEGDRAHDTEYHHADAEDREHEHHEHAEHAHDHEHAHDEHDHADHDHGDVQ
                     ASETLAAVTLPDTAAIEVAAAAPDRAEEAHEAHAHDDRHEAYGHEDTIQADQAPREGG
                     GAEMTEHADAEAAFDLGEQAAPSPGDQIQDDEDEPDDAEEEMVESVGGDDVLEEVPER
                     AFRPRRQYKIQEVIKRRQVMLVQVVKEERGNKGAALTTYLSLAGRYAVLMPNTARGGG
                     ISRKITSAQDRSRLKEVVQDLDVPEGMGIILRTAGASRTKPEIKRDFEYLIRMWETVR
                     DMTLKSQAPTLVYEEGSLIKRSLRDLYTKEIDEILVAGEAGYQEARDFMKMLMPSNVR
                     AVKQYRDGQPLFSRMGVESQLDAMFSPTVQLRSGGYIVINQTEALVSIDVNSGRSTRE
                     HHIEDTALKTNLEAAEEVARQLRLRDLAGLIVIDFIDMDEKRNNRAVERKLSDCLRQD
                     RARIQVGRISHFGLLEMSRQRIRASVLESSTEPCPQCGGSGHVRSVSSVALQLLRGLE
                     ETLMKGATHNLVVRTRTDVALYVLNHKRGHLRDLESSFKVTLAVIADPTVSGQQSFVI
                     DRGEQVHTLEAAKALLAAQVAAFPSQSEEALDEEEPIDIEGESEVETEETEGLAEDSP
                     AGEDSGSEAEADGYKRKRRRRRRGRSGEMREGAPARDDHDTPRVAFEAVSIPGVIAEE
                     SDADEDESEEQPGQPGQLRADHSAAGGERRPRRRGRRGGRRRRGGPEDGLAGSIVDEL
                     RPAPAPEAASAVADFDGAAAEQPAPESQPQPIAPTPEPQDPDRAQQEAAHLSAEVETA
                     QEAERAARRRSTVREKVSFLVDQRDPTAPATPSQPEPAAPPAPAAEPSSETTTDTQPR
                     RAGWWSRRFGGGE"
BASE COUNT          688 a         1420 c         1423 g          861 t
ORIGIN      
        1 gcacgctgtc aaagacgtca acctgcagat ccgcgacaag gagttcgtgg tgttcgtcgg
       61 accgtccggc tgcggcaaga ccacgacgct caggatgatc gccgggctgg aagcgatcag
      121 ctccggcgat atctcaatcg acggcaacgt gatcaatcaa cttgcgccca tggaccgcga
      181 catcgcgatg gtgttccaga actacgcgct ctacccgcat atgagcgtat acgacaacat
      241 ggcgttcggc ctgaagatgc gcaaattcga aaggcccgag attgagaagc gcgtgcggga
      301 ggcggccgac atcctcggca tcggcgaatt gctaaagcgc aagccgcgtc agctctccgg
      361 cggccagcgc cagcgggtgg ctttgggccg cgccatcgtg cgccaccccc gcgtgttcct
      421 gttcgacgag ccgctgtcga acctcgatgc caagctgcgg gtgcagatgc gcgttgagct
      481 caagaagctg catctgcgtc tcggtaccac cgccatttat gttacccacg atcaggtcga
      541 ggccatgacg ctgggtgacc gggtcgtcgt catgaaggac ggcgtggtgc agcaggtggg
      601 ggagccgctc gagctataca atcagccagc caacaaattc gtcgccggct tcatcggctc
      661 gccggcgatg aatttcgccg ccgttaccgt gaccgaggcg aacggatcgc tgatggccga
      721 gaactccggc ttgcgcatca agctgccaga agagaccgcg cagcgcttgc gcggccatgt
      781 cggccacgag gtcacgctcg gcgtgcgccc ggaagacctc accgtggccg gcgccgccga
      841 tatcgaccag ccgtgcttcg atgccgtgat cgaagtggtc gagcaactcg gctcggaaat
      901 cctgctcgat atgaaggtcg gcgagggcgt gatggtggcg agcgtcgagc cgaccgcgcg
      961 cgtaaaggtg cgcgacagac tgcgcgttgc catgcgcccc tccaggctcc acgttttcga
     1021 tgcgaagacc gaggcggcaa tctaacgccg gttttgccgg gcggtagagt gagcggagtc
     1081 attccgggcc ggccaggctg aacccggaat ctccagatga tccaacgaag agattttcgg
     1141 gatcgcgcta aggcgcggcc cggaatgacg accgagctat tcgccgccgc caaaacggcg
     1201 cgaccaccac cccgcgcggc ggggctgggt atcggttgtc gtctccgaag agggctcggc
     1261 ggccggggcg gggggcgccg ccggctccgg ctggctggga gtagcgggtg ccgtcggatc
     1321 acgctggtcg accagaaagc tcaccttctc gcgcacggtc gaacgccgcc gtgcggccct
     1381 ttcggcctct tgagcggtct ccacctcggc tgacaaatga gccgcctcct gctgagcccg
     1441 atccggatcc tgcggctccg gcgttggtgc gatgggttgg ggctgacttt ccggcgcagg
     1501 ttgctcggcc gcagcaccgt cgaaatctgc cactgcgctc gctgcttcag gggccggcgc
     1561 cggcctgagt tcgtcgacga tcgatccggc cagaccgtct tccggcccgc cgcgccgccg
     1621 acgtccacca cgccgtccgc gacgccgtgg ccgccgctcg ccgccggctg ctgagtgatc
     1681 ggcgcgaagc tgtccaggct ggccgggttg ctcctccgat tcgtcctcgt cggcgtcgct
     1741 ttcttcggcg atcacgccag gaatggagac cgcctcaaat gccacacgcg gggtgtcatg
     1801 gtcatcgcgc gcgggcgccc cttcccgcat ctcgccggaa cgaccgcggc gccggcggcg
     1861 gcgcttgcgc ttgtagccat ccgcttcagc ctcgctgccg ctgtcctcgc cggccgggga
     1921 atcctcggcc aggccttcgg tctcctcggt ttccacttcg gattcgcctt cgatgtcgat
     1981 cggctcttcc tcgtcgagcg cttcctctga ctgcgacggg aaagccgcaa cctgcgccgc
     2041 cagaagcgcc ttggcggcct ccagcgtgtg cacctgctcg ccgcgatcga tgacaaacga
     2101 ctgctggccg ctcacggtcg ggtcggcgat cacggccagc gtcaccttga aactgctttc
     2161 gagatcgcgc aggtggccgc gcttgtggtt gagaacgtaa agcgcaacgt cggtccgggt
     2221 gcgcaccacg agattgtggg tcgcgccctt catcaatgtt tcctcgagcc cgcgcagcaa
     2281 ctgcaacgcc accgaagata ccgaacgcac atggccgctg ccgccgcatt gcgggcaggg
     2341 ttccgtcgaa ctttccagca cgctggcgcg aatgcgctgc cgcgacattt cgagcaggcc
     2401 gaaatgcgag atgcgcccga cctggattcg ggcgcggtcc tgccgcaggc aatcgctgag
     2461 cttgcgctca accgcgcgat tgttgcgctt ctcgtccatg tcgatgaagt cgatgacgat
     2521 caggccagcg aggtcgcgca agcgcagctg ccttgcgact tcctcggcgg cctcgaggtt
     2581 ggtcttgagc gcggtgtcct cgatgtgatg ctcacgggtc gagcggccgg aattgacgtc
     2641 gatcgaaacc agcgcttcgg tctgattgat tacgatgtag ccgccggatc gcaattgcac
     2701 ggtcggcgag aacatcgcat cgagttgact ctcgacgccc atccgcgaga acagcggctg
     2761 gccgtcgcga tattgtttga ccgcgcgcac gttcgagggc atcagcatct tcatgaaatc
     2821 gcgggcttcc tgatagccgg cctcgccggc gaccaggatt tcgtcgatct ccttggtgta
     2881 gaggtcgcgc agcgatcgct tgatcagcga gccctcctca tacaccaggg tcggcgcctg
     2941 cgacttcaag gtcatgtcgc gcacggtttc ccacatgcgg atcagatatt cgaagtcgcg
     3001 cttgatttcg ggcttggtgc gggaagcgcc agcggtgcgc agaatgattc ccatcccctc
     3061 gggcacgtcg agatcctgca ccacttcctt cagccgcgag cgatcctgcg cggaggtgat
     3121 cttgcggctg atgccgccgc cgcgcgcggt gttgggcatc aacaccgcat agcggccggc
     3181 cagcgacaaa taggtcgtca gcgcggcgcc cttgttgccg cgctcttcct tgaccacctg
     3241 caccagcatc acctgacggc gtttgatgac ctcctgaatc ttgtactgac gccgcggccg
     3301 gaaggctcgc tccggcacct cttccaggac gtcgtcgccg ccgaccgatt cgaccatttc
     3361 ttcctcggcg tcgtcgggct catcttcatc atcctggatc tggtcgccag gcgacggcgc
     3421 cgcctgctcg ccgagatcga atgcggcctc ggcatcggca tgttcggtca tttcagcacc
     3481 gccgccttct cgcggcgcct gatcggcctg gatggtgtct tcgtgtccat aggcttcgtg
     3541 acggtcatcg tgcgcgtggg cttcgtgggc ttcttcagcc ctgtcaggcg cagcggccgc
     3601 cacttcgatg gcagccgtat cgggcagggt caccgccgcc agcgtctcgc tcgcctgaac
     3661 atcgccgtgg tcgtgatcag catgatcgtg ctcgtcgtga gcatgttcat gatcgtgcgc
     3721 gtgctcggca tgttcatgat gttcgtgttc acgatcttcc gcatcggcat gatgatattc
     3781 ggtgtcgtgg gcgcgatcgc cttcgccgtg caggttttgc gcgggttctt gcgggtgttg
     3841 ttggccttct tcctgacgct gctgatcctc ataggactta gcggcctcgg gcggctcggt
     3901 gctcgcgtgc tcgacgatct cgctctgaat gcgctcgcca ttgccgcgac ggcgcgaatt
     3961 gcggtggcgc gggcggcggc gattggagcg ggtctcgctc tcctcctcgg cctcgcgatg
     4021 ggcgcgttcg tccgcctcga tcagggcctg gcgatcggcg acgggaattt gataatagtc
     4081 cggatgaatt tcgctgaagg ccagaaagcc gtgacggttg ccgccgtact cgatgaaggc
     4141 cgcctgcagc gacggctcta cgcgggtgac cttggcgaga tagatgttgc cgcgcagttg
     4201 cctgcgctgg gcggtctcga aatcaaattc ttcgacgcga ttgccgcgga ccacgacgac
     4261 ccgggtctct tccgggtggg tggcgtcgat caacattttg ttgggcattt tggaactcat
     4321 gacggcggcg ggcgcgcagt accgtgagcg cgtattgcgc ggccgggtga cgcgacggtc
     4381 cacctgattc gg
//
LOCUS       DATNOI010000643         2720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1676792,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000643 DATNOI010000000
VERSION     DATNOI010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676792"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VN968_25995"
     CDS             <1..490
                     /locus_tag="VN968_25995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL32718.1"
                     /translation="IGANIAFGKAGATEQEIVAAAKAACAHEFIMGFPLGYDTPVGEH
                     GTQLSGGQRQRVAVARALIKNAPIILLDEATAALDSESEKQVQEAIEHLCQNRTTIVI
                     AHRLHTIMHADAILVVEGGEIVERGRHDDLLRRSGRYASFFRLQHRDAGPVALAPVSA
                     TA"
     gene            536..1237
                     /locus_tag="VN968_26000"
     CDS             536..1237
                     /locus_tag="VN968_26000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXL32719.1"
                     /translation="MSATSFVIPALPQPSIPVAGETKSFPVRRIWCVGRNYLEHIREM
                     GNDERAPPFFFAKHADMLVPDGATIPYPPLTKDLHHEVELIVAMKSGGLNIPADKALD
                     HVYGYGVGIDLTRRDLQIASRKKERPWEVGKSFDYSAPCSALQPASKIGHPSKGRIWL
                     TVNGTEQQKGDLTELIWSVPEIIWQLSQQVALAAGDIIMTGTPAGVSALNPGDKIECG
                     VDGVGTLKVAIGEPA"
     gene            1406..>2720
                     /locus_tag="VN968_26005"
     CDS             1406..>2720
                     /locus_tag="VN968_26005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin-coupled sensor protein"
                     /protein_id="HXL32720.1"
                     /translation="MTPDWNAIRLDLLQVDEPMRAALREMRPFFAKCLPGILARYYDK
                     VQHYDPSSGIFKNGTMQEAIRMQLQHWELIAACNFGPDYLNSATRFCELNQRAGVAPQ
                     WYVGGRQMFIADQLMKAADNEIAIPRFGRAAQAARDRKALMLNAIAKAMMMDTQNIIA
                     AYFGSNRELRKGAIAEASDRFRTIITSLSTASNELEGTARSLSDSADNTTRLATVVAN
                     ASEDASNNVQSVASATEELASSVREISGQVQESNRIAASAVQQADETDARINALSQAA
                     NRIGDVVKLITSVAEQTNLLALNATIEAARAGEAGRGFAVVAQEVKALAAQTAKATDE
                     IGNQIAGMQTATQEAVGSIKMISSTIGKISEITSAISAAIEEQGAATQEISGNIQRTA
                     AGTTQVAGTVAEVSQGATQTGAASGQLLLSARQLSHTTASLQAEID"
BASE COUNT          536 a          922 c          816 g          446 t
ORIGIN      
        1 catcggtgcg aacatcgcgt tcggcaaggc cggtgccacg gaacaagaga tcgtggctgc
       61 ggcaaaagcg gcctgcgcgc acgaattcat catgggcttt ccgctcggct acgacacccc
      121 ggtgggcgag cacggcaccc agttgtccgg cggccagcgc cagcgcgtcg ccgtcgcgcg
      181 cgcgctgatc aagaacgcgc cgatcatcct gctcgatgaa gccaccgcgg cactcgattc
      241 ggaatccgaa aagcaggtgc aggaggcgat cgaacatctc tgccagaacc gcaccaccat
      301 cgtcatcgcc catcgccttc acaccatcat gcacgccgac gccattctgg tggtcgaggg
      361 cggcgagatc gtcgagcgcg gccggcacga cgatctgttg cgccgcagtg ggcgctacgc
      421 ctcgttcttc cgcctgcagc atcgggatgc agggcctgtc gctctggcgc cggtcagcgc
      481 aacggcttaa agctaccatc gagttcccgt ccccaacccc cgccgagatc ccctcatgag
      541 cgctacatcc tttgtcattc ccgccctgcc ccagccttcg attccggtcg ccggcgaaac
      601 gaaatccttt ccggttcgcc gcatctggtg cgtcggacgc aactatctcg agcacatccg
      661 cgagatgggc aatgacgagc gcgcgccacc gttcttcttc gccaagcacg ccgacatgct
      721 ggtcccggac ggcgccacga tcccctaccc gccattgacc aaggatttgc accacgaggt
      781 tgaattgatc gtcgccatga agagcggcgg cctcaacatc cccgccgaca aggcgctcga
      841 tcatgtctac ggctatggcg tcggcatcga cctcacccgc cgcgacctgc agatcgcctc
      901 gcgcaagaag gagcggccgt gggaagtcgg caaatccttc gactattccg cgccctgctc
      961 ggcgctgcag cccgcctcca agatcggcca tccctcgaag ggcaggattt ggctcacggt
     1021 gaacggcacc gaacagcaga agggcgacct caccgaactg atctggagcg tgccggaaat
     1081 catttggcaa ctctcgcaac aggtcgcact cgccgccggc gatatcatca tgacgggaac
     1141 gccggctggc gtctccgcgc tcaaccctgg cgacaagatc gaatgcggtg tcgacggcgt
     1201 cggtacgctg aaggttgcga tcggcgagcc ggcctgacgc ctctgcgcgc ctaagcgccg
     1261 aaaccgaggc cccggaccct gttcggggcc tttttcgcgt ccgaatttcg gcgcttctag
     1321 gtagaactac ggagaaattc gcattctgtt aaccatgcgg actttagatg gtctgaccgc
     1381 agcgccgctt gacctggagc cgcagatgac ccccgactgg aacgcaatcc gcctcgattt
     1441 gcttcaggtc gatgagccga tgcgggcggc cttgcgcgag atgcggccgt ttttcgcgaa
     1501 gtgcctgccc ggcattctgg cgcggtacta cgacaaggtg cagcactacg atccctcctc
     1561 cggcatcttc aagaacggaa cgatgcagga ggcgatccgc atgcagctgc agcactggga
     1621 gctgatcgcc gcctgtaatt tcggccccga ttatctgaat tcggcaacgc ggttttgcga
     1681 gctcaatcag cgcgccgggg tggcgccgca atggtatgtc ggtggccggc agatgttcat
     1741 cgccgaccaa ttgatgaagg cggccgataa cgaaatcgcg attccgcgct tcgggcgggc
     1801 cgcacaggcc gcgcgcgacc ggaaggcctt gatgctgaac gccatcgcca aggccatgat
     1861 gatggacacc cagaacatca tcgccgccta tttcggttcc aaccgcgagc tccgcaaagg
     1921 cgcgattgcc gaggccagcg accgcttccg caccatcatc acctcgttgt cgacggcgtc
     1981 gaacgagctg gaaggcaccg cacgctcgct gagcgacagc gccgacaaca ccacgcggct
     2041 tgcgaccgtg gtcgccaatg cctcggaaga cgcctccaac aatgtgcaat cggtggcatc
     2101 ggcgaccgaa gaactcgcca gttcggtgcg cgagatttcg gggcaggttc aggagtccaa
     2161 ccgcatcgcc gccagcgccg tgcaacaggc ggatgaaacc gacgcgcgca tcaatgcgct
     2221 gtcgcaggcc gccaaccgga tcggcgacgt cgtcaagctg atcacctcgg tcgccgagca
     2281 gaccaacctt ctcgcgctta acgccaccat cgaggcggcc cgcgccggcg aagccggccg
     2341 tggcttcgcc gtggtcgcgc aggaggtcaa ggcgctcgcc gcgcagaccg ccaaggccac
     2401 cgacgaaatc ggcaaccaga tcgcgggcat gcagaccgcg acccaggagg cggtcggctc
     2461 gatcaaaatg atcagctcga ccattggcaa gatttccgag attaccagcg cgatttcagc
     2521 ggcgatcgag gaacagggcg ccgccaccca ggaaatctcc ggcaatatcc agcgtaccgc
     2581 tgcaggaacc acgcaagtcg ccggcactgt cgccgaagtc agccagggcg ccacccagac
     2641 cggcgcggcg tcgggtcaat tgttgttatc ggcaaggcaa ttgtcgcata ccaccgccag
     2701 cctgcaggcc gagatcgacg
//
LOCUS       DATNOI010000644         3881 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_209780,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000644 DATNOI010000000
VERSION     DATNOI010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209780"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VN968_26010"
     CDS             <1..1140
                     /locus_tag="VN968_26010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012755772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HXL32721.1"
                     /translation="GKHCKDDPHCFNRYHFAIKPAARVNPEQLFVLETRDALDSDLTF
                     DSTAQDVAAANLNLVHPLTGPVYIEGARRGDALAVTVVDIAPDDFGYTTIVPGFGFLR
                     DVFRDPFIVHWNLTRLEARSKDLPGISVPMNAFMGTVGVLPGEPELETWLKREKALAD
                     AGGAALTPQPVDALPADLCGPNGTAKDKCVRTVPPRENGGNLDTKETVVGTTLLFPCF
                     IDGCGLFAGDVHFAQGGGEVAGTAIEMGAKVTLQAKVIPGMASLLTTMHFEGGSQLKS
                     LAPGSFYAVSGLPLKPEGELPVFDTYLGGQKIAPLANLSEDVSLAARNATLNMIDFLV
                     KTKGLSREQAYVLVSVAVDLHIAQLVDVPNLGVTAILNRDVFKPQ"
     gene            1206..1811
                     /locus_tag="VN968_26015"
     CDS             1206..1811
                     /locus_tag="VN968_26015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012755771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32722.1"
                     /translation="MKRRTILTSVASAAALVLAGHRTFAVAGPDRQQRPIRPRFADLN
                     PDRMWPVYRRYHLLIVGQRDDESGSALAAATVDVLMRFLPNSRAMLVRAADARRVGVL
                     VATDQQDVAIMAAGSAEALFLGKPPFDDIRDAPLRVIVSFGSHSLVCRPSFMARHAYL
                     IARTLAEHKDAFPTPAGVPDGVVPAHRGSHAFFAGEEMPND"
     gene            1963..2619
                     /locus_tag="VN968_26020"
     CDS             1963..2619
                     /locus_tag="VN968_26020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32723.1"
                     /translation="MIDIERANGLVSAADEEVALAKTAAGPGPEGMARFALGMVLVEA
                     TNILNRDLAAHSGRLTVNAELLLKALVQRDLAPRLDDAIGRYRLPRTPLEEAIRLSPE
                     APYSLHARFELLKAGFYESFVLDPFQLVGIGLDDLDHQIAEAKALALAIASGDDAEEA
                     AFIHAIDLARASQLAPPKERRAYAGKARTALGAFSEAYPQSIRAATAGVIIKRLGGAE
                     "
     gene            2645..3823
                     /locus_tag="VN968_26025"
     CDS             2645..3823
                     /locus_tag="VN968_26025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HXL32724.1"
                     /translation="MNWALRRTRFIQSLRMRPDRARLNSGPATTPSRAWLALASVAVA
                     AIATWEAQAHNAGVSTSRIVVHGRTVEVEINALGRDYEKSAGVRITEAGSGEVNRVAL
                     AVMAPSVLTYIGDHVAVFAGRQQCAPRPGTALPADTHVLVTVAWTCPLDGGELRYRAT
                     LFHDVDPTARHLAIIATESGERELALDSRAPEVALSGAGSSALQVVGRFVQAGIEHIF
                     LGYDHIAFLAAIVLWAQRLWPVIKIVTAFTIAHSITLSLAALQIVVFPSAIVEPAIAA
                     TIIFVAVENFFSRNVDGRWRVTFVFGLIHGFGFASALQEIGLPANAAVPALAAFNIGV
                     EIGQVVIVALIFPLLLWSDRIGHRAAARKGRHPAVVYFCSAAILSAGLYWLMVRTVLP
                     "
BASE COUNT          640 a         1235 c         1223 g          783 t
ORIGIN      
        1 ggcaagcact gcaaggacga tccgcactgc ttcaaccggt atcatttcgc aatcaagccc
       61 gccgcccgtg taaatcctga gcagcttttt gtactcgaaa cgcgcgatgc gctcgattct
      121 gacctcacct tcgattcgac ggcgcaggat gtggcggccg ccaatctcaa cctcgtgcat
      181 ccgctgaccg ggccggtcta cattgaaggg gcgagacggg gcgacgcact cgccgttacc
      241 gtggtggaca tcgctcccga cgatttcggt tacactacga tcgtgccagg atttggcttc
      301 ttgcgtgatg tcttccgcga tcctttcatc gtgcactgga acctgacgcg tctcgaagcg
      361 cgttcgaagg atctgccggg aatctccgtc cccatgaatg cgttcatggg cacggtcggc
      421 gtcttgccgg gcgagcctga gcttgagacg tggctcaaac gggagaaggc gctcgctgac
      481 gcgggcggcg cggcgttgac ccctcagccg gttgatgccc tgccggcgga tctttgcggt
      541 cccaatggta cggcgaagga caagtgtgtt cgcaccgttc cgccacggga gaacggcggc
      601 aatctcgaca ccaaggagac ggtggtcggc accacgctgc tgtttccttg ctttatcgac
      661 ggctgtgggc ttttcgcggg tgacgttcat ttcgcccagg gcggcggcga ggtggcgggc
      721 accgccattg agatgggcgc aaaggtgacg ctccaggcca aggtgatccc gggcatggct
      781 tcgctgctta cgaccatgca cttcgaaggc ggctcgcagc taaagagcct cgctccgggg
      841 agtttctacg ctgtctcggg gcttccgctc aagcccgagg gcgaattgcc ggtgttcgac
      901 acctaccttg gtggccagaa gatcgctccg ctggcaaacc tctcggagga tgtcagtttg
      961 gcagcgcgaa acgccacgct caacatgatc gactttctgg tcaagacaaa gggtcttagc
     1021 cgcgagcagg cttacgtact ggtgagcgtg gccgtcgatc tccacatcgc gcaactcgtc
     1081 gatgtcccga acctgggtgt cacagcgatc ttgaaccgcg acgtgttcaa gccgcaatga
     1141 cgggtgcctg agccaatcgt tggtgcaggc tcggcatcgc accgctgcct tggagcttgg
     1201 cgagtatgaa gcggcgcacg atcctcacgt cggttgcgag tgcggcggcg cttgttcttg
     1261 ccgggcacag aacgtttgcg gtcgcgggcc ctgatcggca gcaacggccg atcaggccgc
     1321 ggtttgccga cttgaatccg gaccgtatgt ggccggtcta ccggcgctac cacctgctga
     1381 tcgtcggcca acgcgatgat gaaagcggca gtgctcttgc agcggcgacc gtcgacgtgc
     1441 tgatgcgatt tctcccgaac tcgcgcgcca tgctggtccg cgctgcggat gctcggcgcg
     1501 tcggcgtgct cgttgccacg gatcaacagg acgtcgcaat catggcggcg ggaagtgcgg
     1561 aggccctctt tctcggcaag ccgccctttg acgatatccg cgacgcgccg ttgcgggtaa
     1621 tagtatcatt tgggagccac tcccttgtgt gccgcccgag tttcatggca cgccacgcct
     1681 atctgatcgc caggacgctg gctgaacaca aagacgcttt tcccacgccc gccggtgttc
     1741 ccgacggagt cgtcccggcg caccggggat cgcacgcctt tttcgccggt gaagagatgc
     1801 ccaatgactg atgactcggc cacggcaaac gcgtcccgca cggggcaatc gctaaccgcc
     1861 gccagcccgc gatgcgcagc cttccggggt cccgggccca ggctcgttct tttcgttctc
     1921 ctcttttcga ctcttggcgc ccgcacgctg gtcgctcatg atgtgatcga catcgagcgg
     1981 gcaaacgggc tcgtctccgc tgccgacgag gaggtcgcgc tcgccaaaac tgccgccggg
     2041 ccaggacctg agggcatggc gcgatttgca ctcggtatgg ttctagtcga ggcgaccaac
     2101 atcctcaacc gcgatctcgc ggcgcacagc ggtcggctta cggttaacgc cgaacttttg
     2161 ctgaaggctc tcgtccagcg cgatctggcg ccccgcttgg acgatgcgat cgggcgctac
     2221 cgcctgccga gaaccccgct cgaggaggcc attcgcctct cacccgaagc accttattcg
     2281 ctgcacgcgc ggttcgagct cctgaaagcc ggcttctacg agagcttcgt actcgatcct
     2341 tttcagcttg tcggcattgg gttggacgat cttgaccacc agatcgccga ggccaaggcg
     2401 ttggctttgg ccatcgcgag cggggacgac gccgaggagg cagcgttcat tcacgccatc
     2461 gatcttgcgc gggcctcaca acttgcgccg cctaaggaaa ggcgcgctta cgccggcaag
     2521 gcgcggacgg cgctcggggc gttctcagaa gcctaccccc agagcattcg tgccgcgacg
     2581 gccggcgtaa tcattaagcg cctgggcggt gccgaatgat tggcgaccga cgccagcgac
     2641 gccaatgaac tgggcactcc gccgaacgcg ctttatacaa agcctccgga tgcgcccaga
     2701 ccgtgctcga ctcaacagcg gtcccgccac gacaccgtct cgcgcctggc ttgccctggc
     2761 cagcgttgcc gtcgctgcca tcgccacttg ggaggcgcag gcgcacaatg ccggggtcag
     2821 cacctcgcgc atcgtggtcc acggccgcac ggtcgaagtg gaaatcaatg cccttggtcg
     2881 cgactacgaa aagtcggccg gggttcgaat caccgaagcg ggatcgggcg aggtcaaccg
     2941 tgtggcgctg gcggtcatgg cgccgtcagt gctcacctac attggcgacc acgttgcagt
     3001 ctttgccggc aggcagcaat gcgcgcctag gccaggaacg gcactgccgg ccgacaccca
     3061 cgttctggtg acggtggcat ggacctgtcc attggatggc ggcgagctgc gctatcgcgc
     3121 gaccctgttt cacgacgtgg atcctaccgc acgacatctg gctatcatcg caaccgagag
     3181 cggcgagcgc gagcttgccc tcgacagcag agcgcccgag gtcgctctgt ccggtgcggg
     3241 gtcgtcggca ctccaggtgg tcgggcggtt cgtccaggcc ggtatcgagc acatctttct
     3301 cggctacgat cacatcgcat tccttgccgc gatcgtgctt tgggcgcagc gtttgtggcc
     3361 ggtgatcaag atcgtcaccg ccttcacgat tgctcactcg atcacgctgt cccttgctgc
     3421 gctgcagatt gtcgtattcc caagtgccat cgtcgaaccg gcgatcgccg cgacgatcat
     3481 atttgtagcg gtcgagaact ttttctcgcg caatgtggac ggtcgctggc gggtgacgtt
     3541 cgttttcggg ctcattcacg gcttcggctt cgccagcgcg ctgcaggaga tcgggctgcc
     3601 agcgaatgcg gccgtgcccg cgctcgcggc tttcaacatc ggcgtggaga ttgggcaggt
     3661 cgtgattgtg gccctcatct ttccacttct gctgtggtcg gatcggatcg gtcaccgggc
     3721 cgcggcacgc aagggacggc atccggcggt ggtctacttc tgttcagcgg cgatcttgtc
     3781 ggccgggctt tactggctca tggtacgcac cgtgctcccc tgaagcatcg gccagttgct
     3841 ttagcgccat gtgcgatccg gccttggtcg acggctttgt c
//
LOCUS       DATNOI010000645         2727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1340290,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000645 DATNOI010000000
VERSION     DATNOI010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340290"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VN968_26030"
     CDS             <1..586
                     /locus_tag="VN968_26030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL32725.1"
                     /translation="VKSGVSVITPNPKTSGGARWNYLAAWGYALKKSGSAEKAKQFVG
                     DLYKNVPVLDTGARGSTVTFVERGVGDVLLAWENEAFLAQREFGKDKFEIVSPPLSIL
                     AEPPLSVVDAVADKKGTRAVAEAYLKYWYSREGQEIAARNSYRPRDSEIAKEYEKSFA
                     KVELFTIDDVFGGWAKAQKEHFAEGGIFDQIYKN"
     gene            613..1449
                     /gene="cysT"
                     /locus_tag="VN968_26035"
     CDS             613..1449
                     /gene="cysT"
                     /locus_tag="VN968_26035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HXL32726.1"
                     /translation="MSGGAGRRSTLPGFGLTMGLTLTWLSVIILIPLAGLFLKTFELS
                     LDQFWDILTNRRTVNALRISFGLAFGAAAVNLVMGMVIVWALVRYSFPGRRIFDAIVD
                     IPFALPTAVAGVALTSLFAQKGWLGAPLAALGIKVAFTPVGIFVAMIFIGIPFVVRTV
                     QPVLLDLDAEIEEAAASLGANRWHTVSKVILPSMIPALLTGFALAFARAVGEYGSVIF
                     IAGNLPNVSEIAPLLIVIRLSEFRYADATAIAVVMLVASFLIIFAVNRIQRWAQTRIP
                     AH"
     gene            1563..2432
                     /gene="cysW"
                     /locus_tag="VN968_26040"
     CDS             1563..2432
                     /gene="cysW"
                     /locus_tag="VN968_26040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HXL32727.1"
                     /translation="MSELQSSLRSARDDLRTEPRPIRIVIIAIAVIFLTVFVVLPLVV
                     VFAQAFSKGIGAYFAALSDSEALAAIRLTLLVAAISVSLNLVFGVVAAWAIAKFDFRG
                     KTFLISLIDLPFSVSPVISGLVFVLLFGGQGYWGPWLQAHNIHVLFAVPGIALATIFV
                     TFPFVARALIPLMQEQGTLEEEAAISLGASGLQTFFRVTLPNIKWGVLYGVLLCNARA
                     MGEFGAVSVVSGHIRGETNTMPLLVEILYNEYQFVSSFAIASLLAMLALITLVAKTIL
                     ERHLDEGQYPRGD"
     gene            2422..>2727
                     /locus_tag="VN968_26045"
     CDS             2422..>2727
                     /locus_tag="VN968_26045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL32728.1"
                     /translation="MAIEVKNIVKRFGTFAALDGVDLKVANGELLALLGPSGSGKTTL
                     LRIIAGLDWPDAGEVSFDGENALARGASERHVGFVFQHYALFRHMTVFENVAFGLRVQ
                     "
BASE COUNT          491 a          778 c          841 g          617 t
ORIGIN      
        1 ggttaaatcc ggcgtcagcg tgatcacgcc gaatccgaaa acttccggtg gcgcgcgctg
       61 gaattatctt gccgcctggg gctacgcgct gaagaaatcc ggctccgccg aaaaggcgaa
      121 gcaattcgtc ggcgacctct acaagaacgt gccggtgctg gacaccggtg cgcgcggctc
      181 gaccgtcacc ttcgtggagc gcggcgtggg tgacgtgctg ctggcctggg agaatgaggc
      241 tttcctcgcg cagcgcgaat tcggcaagga caagttcgag atcgtgtcgc cgccgctgtc
      301 gatcctggcc gagcccccac tgtccgtcgt cgacgcggtt gccgacaaga aaggcacccg
      361 ggcggtggcc gaggcttacc tgaaatactg gtattccagg gaaggtcagg aaattgccgc
      421 acggaattcc tatcgtccgc gcgactcgga aatcgcgaaa gaatatgaaa aatcattcgc
      481 gaaagtcgag ctattcacga tagatgacgt ttttggcggc tgggccaagg cgcagaagga
      541 acactttgcc gaaggcggca ttttcgacca gatctacaaa aactgatctg gccgaaggga
      601 acaggggcta ttgtgagcgg gggggcggga cgacgcagta cattgccggg cttcggtctc
      661 accatggggc tgacgctgac atggctttcc gtcatcatcc tgataccgct tgcaggcctg
      721 tttctcaaaa ccttcgaact gagcctcgat caattctggg acattctcac caaccgacgc
      781 accgttaatg cgctgaggat ttcgttcggc ctcgccttcg gcgccgccgc cgtcaatctg
      841 gtgatgggga tggtcattgt ctgggcgctg gtgcgataca gctttccggg gcgccgcatc
      901 ttcgatgcca tcgtcgacat tccatttgcg ctgcccaccg cggtcgccgg cgtcgcactg
      961 acctcgctgt tcgcgcaaaa aggctggctc ggggcgccgc ttgccgcact cggcatcaag
     1021 gtggcattta ctccggtcgg aattttcgtc gcgatgattt tcatcggtat tccgttcgtg
     1081 gtgcggacgg tccagccggt gctgctcgat ctcgacgccg agatcgagga ggccgccgcc
     1141 agcctcggcg ccaatcgctg gcacacggtt tccaaggtga tcctgccgag catgattccg
     1201 gcgctgctga cgggattcgc gctggccttc gcgcgcgccg tcggcgagta cggttcggtc
     1261 atcttcattg ccggcaattt gcccaatgtg tccgagatcg cgccgctgtt gatcgtgatc
     1321 cgcttgtcgg aatttcgcta tgcggacgca accgcaatcg ccgtggtcat gctggtggcg
     1381 tcattcctga tcattttcgc ggtcaatcga atccagcgct gggcgcaaac ccgaatccct
     1441 gcgcattaga gcatgatccg gaaaagtggg aaccggtttt ccgaaaagat catgctcaaa
     1501 caaagagatg agatcatcat ccgattcaat ccaatcggat catgatccag ggatcgatgc
     1561 agatgagcga gcttcagtct tccctccgtt cggcacggga cgatctgcgt accgagccca
     1621 ggcctattcg cattgtcatc atcgcgattg cggtgatctt cctcaccgtt tttgtcgttc
     1681 tgccgctggt tgtggtgttt gcgcaggcct tttccaaagg catcggtgcg tattttgcag
     1741 cactttcaga ttcggaagcg ttggcggcga taaggctgac gctattggtc gcagcgattt
     1801 ccgtcagtct caatctggtg ttcggcgtgg tcgcggcctg ggcaatcgca aaattcgact
     1861 ttcgcggcaa gaccttcctg atctcgctga tcgatctgcc gttctcggtc agcccggtga
     1921 tctccggcct tgtgttcgtg ctgctgttcg gcgggcaagg ctattgggga ccctggctgc
     1981 aggctcacaa catccatgtg ctgtttgcgg ttccgggcat tgcactggcg actattttcg
     2041 tgacgttccc gttcgtggcg cgcgcgctga tcccgctgat gcaggagcag ggcacgctgg
     2101 aggaggaggc tgcgatctcg ctcggcgcct cgggcttaca gactttcttc cgcgtcacgc
     2161 tgccgaatat caaatggggt gtgctgtacg gtgtgctgct gtgcaacgcg cgggcgatgg
     2221 gcgaattcgg cgcggtatcg gtggtatctg gacatattcg cggcgagacc aacaccatgc
     2281 cgctactggt tgaaatcctc tacaatgaat atcaattcgt atcgtcgttc gcgatcgcct
     2341 ccttgctggc aatgctggcg ctgatcacgc tggtggcgaa gaccatcctc gaacggcatc
     2401 tggacgaagg gcaatatccg cgtggcgatt gaagtcaaga atatcgtcaa aagattcggc
     2461 acgtttgccg cgctcgacgg cgtcgatctg aaggtcgcca atggcgaatt gctggcgctg
     2521 ctcgggccgt cgggttcggg caagactacg ctgctgcgga tcattgccgg gctggattgg
     2581 cccgacgccg gcgaagtttc gttcgacggc gagaatgcac tcgcccgcgg ggccagcgag
     2641 cgccatgtcg ggttcgtgtt ccagcactat gcgctgttcc gccacatgac ggtgttcgag
     2701 aacgtcgcgt tcggcctgcg ggtgcag
//
LOCUS       DATNOI010000646         4664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2392329,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000646 DATNOI010000000
VERSION     DATNOI010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392329"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1850..4111
                     /locus_tag="VN968_26050"
     CDS             1850..4111
                     /locus_tag="VN968_26050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXL32729.1"
                     /translation="MSFRRDIITKPIFSWARGVLPPMSDTEREALEAGDVWWDADLFT
                     GNPDWSKLLAYAPATLTDEERAFLHGPVDELCAMLDDWKINWEWRDLPPEAWDFIKRG
                     KFFGMIIPKEYGGLGFSPYAHSEVVRKISSRSVTAAVTVMVPNSLGPGELLMRFGTKE
                     QQQQWLPRLADGREIPCFGLTSPEAGSDAASMIDSGVICKGDFEGREVLGLRLNWHKR
                     YITLGPVATLLGLAFKAYDPDHLVGPREELGITVALIPTHLRGVEIGRRHLPSMQVFQ
                     NGPNRGHDVFIPMDYIIGGEARLGQGWKMLMTALAAGRGISLPSLSAAGAAYAARTTG
                     AYARIREQFNVSIGKFEGIEEPLARIAGTAYLLDAARRLTCAALNQGHHPAVISGIMK
                     LHATERMRVAFDDAMDIHGGKAVIDGPQNYLGNLYRSVPVGITVEGANILTRNLIVFG
                     QGAIRAHPYLLEEMNALGDQDRAKGLDAFDKAFWKHIGHSITTLFRAWGRSWTGGAFA
                     PDAGDATGFYRQLSRYSSAFALCADMALLTLGGALKRKEMLSARFGDILSELYLLSAA
                     LKRWQDEGRQGADFAALEWCMASGFKTIENRFAEILANLPNRFVAVVLKFLVQPFGAR
                     VLGPSDIVIHRCAQLVLEPSAARDRLTPDLSHIDDDRGVARLEKAFLLVTGAEDIAKR
                     MRAAHLHDWNEAVKNGVITKAEGDQLAAAHEAVAKVIEVDDFAPEMLSPIYKKPGEVH
                     QFFQELGETRAAS"
     gene            4111..>4664
                     /locus_tag="VN968_26055"
     CDS             4111..>4664
                     /locus_tag="VN968_26055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HXL32730.1"
                     /translation="MARPVFIIDGSRTPFLKARSGPGPFTPVDLAVQCGRPLLARQPF
                     APDTFDQVILGCVNVIADEMNPARVAALRLGMGEAMVAFTVQINCGSGMQSIDTAYRY
                     IREGVSDLILAGGSEALSYAPLVWPQQGVRWFAGLAGAKGFLGKLAALAKARPAYFKP
                     IIGLERGLTDPITELNMGQTAEVVG"
BASE COUNT          870 a         1465 c         1427 g          902 t
ORIGIN      
        1 ttgtcgcacc tctttcctgc cggcatgatc cgcggcgggc ccgatcgagc caaggtttct
       61 caaacgtaca ccctcaagag gattgattac agcccgcgaa tggcgcggcg cttgtccgaa
      121 gatgctcgct aaccggacat cgacgggcac acgagagtcg acgcgaatga cccaaagctg
      181 actcgttgcc ctgaggccac tcttgatgca ccgggacagt gctgaagagc gcactgagca
      241 aagcaggtga tttccggaaa tgctttgctc gcgtcatgcc tttccgggcg cgatccaaag
      301 ctacctggtg ggcatccggc tgatggattg catgttttgt tgcatcgatt cttgctgccg
      361 tctcatggcc tgaatgacat tttcttgttg ccgcgcggca tcgaaagccg ttcgacatct
      421 gtcgtactcg cttgttccgg gccgaagccc gtcagtccga catttttggt catcaagcag
      481 tgcagtttct tgccgagaag cgcaaccggc caacaccaaa ccaaccgcca tcatcagcgc
      541 tactctcatt ggcacaccat gttccgccgt cagcacatct ttatcacggc atgatgaggt
      601 ctcaactagc cgcggcatca tcggctacaa cgagagtcac atgaggctcc agtgtgcccc
      661 aaaagccgca gtgtgtggtg cttaggcaga ctgtgactct cgcgcgaaaa cccgagaact
      721 tgccgggccg ctatcgcctc cttccttcaa tcgtcacgac ggcgccatcg atccctgttt
      781 acggcatgtt gcgcgggccg gattctggat gccttgcagg acatcccctc gcagcgtcag
      841 cctgtggcac aaatgcggaa gtcaccgccg catcgagcaa tttccgagtt tagatgcaaa
      901 gcagaaaaca tctgctcgca gagagtatat cgcatgttgc acttttcgga cctcgcgcca
      961 tccgaggcgc aggtccggga taccccgatg agcggacatc gtacttgtcg aacgctgcag
     1021 gtctgcatcg cgcaaagatc gttggccgcc actgcgtcga tagcccgcct cgcgctctgc
     1081 atcgccgcat ggtcctgcgc tttcccgaga caaattgacc ccacgggcaa atcacttcgg
     1141 attttccgaa gtcatgtcaa gcccaagaat taaaaatatt tcgctttcgc cgtcgggcaa
     1201 atcaagggca catcaattgt catcctgtcc cgccaagagg ggcgtcggcc atcgtcacga
     1261 acgtgggaca gggagcggtg gacgcggatc cgcctttgac gaacggcgcg gaagcgtacg
     1321 gcgaagacgt gtggtcctga cgcctcggtg ctggcgtcaa gttggcggta agtcccgccg
     1381 atgacggtgg caagaaagcc gatcacaggg gagagcgcgc tataagccgt aaaaccattg
     1441 cgcagggaat gtcggattgc ctccgctgaa cctgtatgct cgtgtgcgca tctttttgtg
     1501 cacattgcac acgagaccgc gggtgcagcg cgcatccggc attccctgcg ccctctcttt
     1561 tcgagagggg caaaacttac ttgcaaacct cgggcagatc gtgccgcgag aacgccgatg
     1621 catatctatt ccctcaaaat tgaatcgccc gtctcggccc cacaagggga gggatcagga
     1681 aggcgtgcgg ccgacactca cattcccgga actgatgcgc ccctgtgccg ttatccagcg
     1741 cctcccaccc catcaatttc tccttatttt caaggcgaag tgttcaccca agaccgccac
     1801 tttccgctaa aatgagcccc cgcgccgccg attcatgagg acgtcagcca tgagcttccg
     1861 acgcgatatc atcaccaagc cgattttctc ctgggcccgc ggcgtgttgc cgccgatgtc
     1921 ggacaccgag cgcgaggcgc tggaggccgg cgacgtttgg tgggacgcgg acctgttcac
     1981 cggcaatccg gactggtcga aattgctggc gtacgccccg gcgacgctga cggatgagga
     2041 acgggccttc ctgcacggcc cggtcgatga gctctgcgcg atgctcgacg actggaagat
     2101 caattgggaa tggcgcgacc tgccgccgga ggcctgggac ttcatcaagc gcggcaaatt
     2161 cttcggcatg atcattccga aggagtatgg cgggcttggc ttctcgccct atgcgcattc
     2221 cgaagtggtg cggaagatct cgtcacgttc ggtgaccgcc gccgtcaccg tgatggtgcc
     2281 gaactcgctc ggccccggcg aactgctgat gcgctttggc accaaggagc agcagcagca
     2341 atggttgccg cgccttgccg acggccgcga gattccctgc ttcggattga ccagtcccga
     2401 ggccggctcc gacgccgcgt cgatgatcga cagcggcgtc atctgcaaag gcgatttcga
     2461 ggggcgcgag gtgctcggcc tgcgtctcaa ctggcacaag cgctatatca cgctcggccc
     2521 ggtcgcgacg ctgctcgggc tggccttcaa ggcctatgac cccgatcatc tggtggggcc
     2581 gcgggaagaa ctcggcatca ccgtggcgct gatcccgacg catctgcgcg gcgtcgagat
     2641 cggccgtcgc catctgccgt cgatgcaggt gtttcagaac ggcccgaacc ggggccacga
     2701 cgtcttcatt ccgatggatt acatcatcgg cggcgaggca cgtctcggtc agggctggaa
     2761 aatgctgatg accgcacttg ccgccgggcg cggcatctca ctgccgtcat tatccgcagc
     2821 gggcgcggcc tatgccgcgc gcaccactgg cgcctatgcg cggattcgcg agcagttcaa
     2881 cgtctcgatc ggaaagttcg aaggcatcga ggagccgctg gcccgcatcg ccggcactgc
     2941 ctatctgctc gatgcggcgc ggcggctgac ctgcgccgcg ctcaatcagg gccatcaccc
     3001 cgcggtcatt tccggcatca tgaaactgca tgctaccgag cggatgcggg tcgcgttcga
     3061 cgacgccatg gacatccatg gcggcaaggc tgtgatcgac ggcccacaga attacctcgg
     3121 caatctctat cgttcggtgc cggtcggcat caccgtcgag ggcgccaaca tcctgacgcg
     3181 caacctgatc gtgttcggac agggggcgat ccgcgcgcat ccttatctgc tggaagagat
     3241 gaacgcgctc ggcgatcaag accgcgccaa aggcctcgac gccttcgaca aggcattctg
     3301 gaaacatatc ggtcacagca tcacgaccct gttccgggcg tggggccgga gctggaccgg
     3361 cggtgcattt gcgcccgacg ccggcgacgc gaccggattc tatcgccagc tctcgcgtta
     3421 ctcctcggcc ttcgcgctat gcgccgacat ggcgctcctg acgctgggtg gcgcactgaa
     3481 gcgcaaggaa atgctgtcgg cgcggttcgg cgatatcctc tcagagctct atctgttgtc
     3541 cgccgcgctc aagcgctggc aggacgaggg ccgacagggg gcggatttcg cggcactcga
     3601 atggtgcatg gcgagtggct tcaagaccat cgagaatcgt ttcgcggaaa tcctcgccaa
     3661 tctgccgaac cggtttgttg ccgtggtgct gaaattcctg gtccagccgt tcggcgcccg
     3721 cgtgctcggt ccgtccgata tcgtgatcca tcgttgcgcg cagctggtgc tggagccgtc
     3781 ggcggcgcgc gaccgcctga ccccggacct gtcgcatatc gatgacgacc gcggcgtcgc
     3841 aaggctggag aaggcgtttc ttctggtcac cggcgccgag gatatcgcca agcggatgcg
     3901 cgccgcccac cttcacgact ggaacgaggc cgtaaaaaac ggcgtcatca ccaaggccga
     3961 gggcgatcag cttgcggcgg cacatgaagc tgttgcgaag gtgattgagg tcgacgactt
     4021 cgcgcccgag atgctgtcgc cgatttacaa gaagcctggc gaagtgcatc agttctttca
     4081 ggagcttggt gaaacaaggg ccgcaagctg atggcgcgac cggttttcat tatcgacggc
     4141 agccggacgc cgttcctgaa agcgcgcagc gggccgggcc cattcacccc ggtcgatctc
     4201 gccgtgcagt gcggccggcc gctgctggcc cggcagccgt tcgcgcccga taccttcgat
     4261 caggtcatcc tcggttgcgt caacgtcatc gccgacgaga tgaacccggc gcgggtcgca
     4321 gccttgcggc tcggcatggg cgaggcgatg gtggcgttca cggtgcagat caattgcggc
     4381 tccggcatgc agtcgatcga cacggcgtac cgctatatcc gcgaaggcgt ctcggacctg
     4441 atcctggccg gcggttcgga agccctgagc tacgcgccgc tggtgtggcc acagcagggc
     4501 gtgcgctggt tcgcaggcct cgccggcgcc aagggatttc tcggcaagct ggcggcgctc
     4561 gcgaaagcac ggccggctta tttcaagccg atcatcggcc ttgagcgcgg gctgaccgat
     4621 ccgatcaccg agctcaacat gggccagacg gcggaagtgg tcgg
//
LOCUS       DATNOI010000647         2301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2585969,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000647 DATNOI010000000
VERSION     DATNOI010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2585969"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="VN968_26060"
     CDS             <1..1624
                     /locus_tag="VN968_26060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXL32731.1"
                     /translation="KYQWQSEIMRFSGRQGKDAARVITGGRAQRQKDYALGDFCKITN
                     YEKLKPDLDLIAAWAPELVIVDEAQRVKNWNTIAARALKRIDSSYAIVLTGTPLENKL
                     EELISIVQFVDQHRLGPTWKLLHEHQVKDEAGRVTGYTGLEKIGQTLAPIMIRRRKSV
                     VLRQLPSRTDQNLLVPMTEMQMDYHRENADEVAKIVHRWRKTRFLSDKDQRRLMIALQ
                     NMRMSCNSTYLLNQETDHGVKADELAALLDELFAEPEAKAVVFSQWTRTHDIVIRRLE
                     ARGLGYVSFNGGVPSEKRPALVERFRDDPACRVFLSTDAGSTGLNLQHASTVVNMDLP
                     WNPAVLEQRIARIHRMGQTRPVQVINFVAKGTIEEGMLSVLAFKRSLSAGILDGGSGE
                     ISLGGSRLNRFMKDVENVTGNMGEGEAVTPAEEVTNIVTADDAGSVEDVSVDVDIGAG
                     ETAIARTDGSGAPPRHTGPDPWQALVQVGGQFIAALAVANDPNAAAHPWIERDPATGA
                     QNLKVPLPSPEAARQLANVLSAFADSLRGKVA"
     gene            1938..2240
                     /locus_tag="VN968_26065"
     CDS             1938..2240
                     /locus_tag="VN968_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HXL32732.1"
                     /translation="MADTASRVPRGRARAERLETRVTAEQKSLIERAAALQGRTLTDF
                     VLTSLQDAARRAIEEHHQLALSVRDSEAFVDALLKPKSVNDRLRDTVRRYRKRAGV"
BASE COUNT          489 a          665 c          755 g          392 t
ORIGIN      
        1 caaataccag tggcaaagcg agatcatgcg cttctcggga cggcagggta aagatgcggc
       61 gcgcgtcatc accggtggcc gggcgcagcg gcagaaggat tacgccctgg gcgacttctg
      121 caagatcaca aactacgaga agctcaagcc cgatctcgac ctgatcgccg cctgggcgcc
      181 cgaacttgtc atcgtcgatg aagcgcaacg ggtgaaaaac tggaacacca tcgcggcgcg
      241 cgccttgaaa cgcatcgata gctcctatgc gatcgtgctg accggaacgc ccttggaaaa
      301 caagctggaa gaactgattt ccatcgtcca gttcgtcgac cagcaccggc tggggccgac
      361 ctggaagctg ctgcacgagc atcaggtcaa ggacgaagcc gggcgcgtca ccggctatac
      421 cgggctggag aagatcggcc aaacgctggc gccgatcatg atccgccggc gcaaatccgt
      481 ggtgttgcgg caactgccca gccggaccga ccagaacctg ctggtgccga tgaccgagat
      541 gcaaatggat taccatcggg aaaacgccga cgaggtggcg aaaatcgtcc accgctggcg
      601 caagaccagg tttctgtcgg ataaggatca gcggcggctg atgattgccc tgcagaacat
      661 gcggatgtcg tgcaacagca cctatctgct gaaccaggaa accgaccatg gcgtcaaggc
      721 cgacgaattg gcggcgctgc tcgacgaatt gttcgccgag cctgaggcca aggcggtggt
      781 gttttcgcaa tggacgcgga cccacgacat cgtcatccgc cgcctcgaag cgcgcgggct
      841 gggctatgtc agtttcaacg gcggcgtgcc atcggaaaaa cgaccggcgc tggtcgagcg
      901 gtttcgggac gaccctgcct gccgcgtgtt cctgtccacc gatgccggca gcacgggcct
      961 aaatctgcag catgcgtcga ccgtggtgaa catggacttg ccgtggaacc cggcggtcct
     1021 cgaacagcgc atcgcgcgca tccatcgcat gggccagaca cggccggtgc aggtgatcaa
     1081 tttcgtggca aagggcacca tcgaggaggg catgctgtcg gtgctggcgt tcaaacgctc
     1141 gctatcggcc ggaattctcg acggcggcag tggcgagatt tcgctcggcg gctcgcgtct
     1201 caaccgcttc atgaaggacg tggaaaacgt cactgggaat atgggtgagg gcgaagccgt
     1261 gactccggct gaggaagtga cgaatatcgt tactgctgat gacgccggat ccgtggaaga
     1321 tgtgagtgtg gatgtagaca ttggcgctgg cgagaccgcg atcgcgcgga cggatgggtc
     1381 tggcgcacca ccacgccata caggccccga tccgtggcaa gccttggtac aggttggtgg
     1441 gcagtttatt gccgcgctcg cggtcgccaa cgatcccaat gcggcggcgc atccatggat
     1501 tgagcgcgat ccagccaccg gcgcgcaaaa cctcaaagtg ccgctgccgt cgccggaggc
     1561 cgcgagacaa ctcgccaatg tgctttccgc gttcgcggac agcttgcggg gcaaggttgc
     1621 gtgatggacg acggcgacag aacgaatgat cgcttcagtt gcctgaccga tgatgcaacc
     1681 tctaaccgcg cggccgacag cagaggaatt gggtgccgtg caaaacaaaa ataagtccaa
     1741 tgatttcaaa agcgcggttt tctgacggta cgcgcgacgg tgcgcgcatt tcgcaacgta
     1801 taaaaattgc gagtgatcaa agggcgttgt tgttccgggt gccgccaggg tttgatgggt
     1861 ccgaagcgat gtcgtcctaa agacgaaggc tcttgaagtg gccggcaatt ggccgtacat
     1921 tagggcagga gaaacccatg gcggatacgg cttcaagagt accccgcggc cgggccaggg
     1981 cagagcgcct tgagacccgg gtcacggcgg agcagaagag cttgattgaa cgggcggccg
     2041 ccctccaggg ccggacactc acggacttcg tgctcacgag cctgcaggat gccgctcggc
     2101 gtgcgatcga ggaacaccac cagctcgcgc tgtcggttcg cgacagcgag gcgttcgtcg
     2161 atgcgctgct gaagccgaag tcggtcaacg accggctccg cgacacggtg cgtcgctatc
     2221 gcaagagagc cggcgtttga cgcgtggctg tcgagacgga acagctccgg gtcgagccac
     2281 cgggttcagg ccatggccga t
//
LOCUS       DATNOI010000648         6928 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1508143,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000648 DATNOI010000000
VERSION     DATNOI010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508143"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="VN968_26070"
     CDS             complement(<1..173)
                     /locus_tag="VN968_26070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HXL32733.1"
                     /translation="MNDTTSATTKPSWWQRLTGGLKRTSSSLGSAVADLVTKRKLDRA
                     MLDDIEDVLLRAD"
     gene            200..922
                     /locus_tag="VN968_26075"
     CDS             200..922
                     /locus_tag="VN968_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HXL32734.1"
                     /translation="MSESLDRPAEVPELTADEIQARVLHRDGLMVVIDKPAGLPVHRG
                     PKGGANLESSFDALRFGLPRPPVLAHRLDKDTSGCLVLGRHRKATASLGLLFKHGRIG
                     KTYWAVVEGGPAEDEGTIDMPLGRLNVERGWWQKPDPDGQKAVTNWRVRGRGNGLAWL
                     DLEPVTGRTHQLRVHTSAAGWPIVGDNIYGSGPRFGEPRLHLHSREIVIPISKNKEPV
                     RIVAPAPEHMRERLRTCGWNGE"
     gene            complement(894..1442)
                     /locus_tag="VN968_26080"
     CDS             complement(894..1442)
                     /locus_tag="VN968_26080"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HXL32735.1"
                     /translation="MAFALSLLLNPEIADAVSARWTRLADAGLSKSMLDLNYPPHVTL
                     AVYDALAVDAAVASLDRAFDNVGQIPVTLTDFSTFGTGSGVCYVALAPSLDLMRLHAA
                     TVAAIGETCRPYYRTGSWTPHCTLATGMSDAEMNSARDFLASDWRSLPGMFETASLVE
                     FVPVVGIKRWSLPTTLRSSHTS"
     gene            complement(1449..2723)
                     /gene="mtaB"
                     /locus_tag="VN968_26085"
     CDS             complement(1449..2723)
                     /gene="mtaB"
                     /locus_tag="VN968_26085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HXL32736.1"
                     /translation="MSVEIVTFGCRLNAFESEVILREAESAGLSDTVVINSCAVTNEA
                     VAQARQSIRRLKRERPELRIVVTGCAAQIEPRMFADMVEVDRVVGNDDKMRGEAWRAA
                     RTALDAGAGFGIAASGKIAVADIMAVRELAPHLVDGFQKGLPRVFVQVQNGCDHRCTF
                     CIIPFGRGHSRSVPMGAVVSQVRALVERGHAEIVLTGVDLTSYGADLPGAPKLGKLTK
                     QILRHVPELKRLRISSIDQVEVDRDLLDVIADDERLMPHLHLSLQSGDDLILKRMKRR
                     HSRAQAIQFCAQVRRLRPDITLGADIIAGFPTETGEMFTRSLDLVEECNLTFLHVFPY
                     SPRPGTPAARMPQVSRERIKDRAKRLRTAAEAALRRRLTSEIGATRQVLIESAGQGRT
                     EHFLPVAISGETPGAVRALTMVGHDGARLTGS"
     gene            complement(2727..3599)
                     /gene="dapF"
                     /locus_tag="VN968_26090"
     CDS             complement(2727..3599)
                     /gene="dapF"
                     /locus_tag="VN968_26090"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXL32737.1"
                     /translation="MSALANQAFAKMNGIGNEIVVVDMRDKSAVITSEEARAIATPGG
                     VHYDQLMVLQPPRLKGTEAFIRIYNNDGSEAAACGNGMRCVARRLFEDTGQTAATFET
                     RAGLLDCWQGPSGDLYTVDMGPPKFGWKDIPLAEEFRDTRSIELQIGPIDAPVLHTPS
                     VVSMGNPHAIFWVEDVNAYDLARFGPLLENHPIFPERANITLAHIVDRGHIAIRTWER
                     GAGLTKACGSAACATAVAAARLKRADRRVQISLPGGELLIEWREGDDHVLMTGTATFE
                     YEGRFDPALFASVA"
     gene            3817..4557
                     /locus_tag="VN968_26095"
     CDS             3817..4557
                     /locus_tag="VN968_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HXL32738.1"
                     /translation="MHRCRFFRLAMVALVPAAAIWLGLGNVLAQTPPSAPPAVSTPTP
                     PAPAPSATPAPAQPATPAPGASSPAAPGTPPATAETPPTLPPATAPVQTADPFGEEIT
                     LTPKTVVVMRGNANWDSAFETLIDSFKALSALLDKQGIKPAGNSMIVYTATDDTGFTY
                     LAEIPVEQEPKNLGKDMSIGKSPEGKALKFVHRGSYDNMDNTYEAITNHLDDKKLEAK
                     DTFVEEYVTDPLKTAEDKLVINVYVPLK"
     gene            4554..5270
                     /locus_tag="VN968_26100"
     CDS             4554..5270
                     /locus_tag="VN968_26100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598830.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HXL32739.1"
                     /translation="MKLLKHRAPLFAAIAAGTLLAAPALAQTAPPAMISVAGEATVSV
                     PPDQAQIDAGVATEAKTAREASDANNAAMGKVLLALNGAGIEEKDYQTSRLSLQPESA
                     PNRSSGPSTIVGYRASNRVTIRLRDVTKVASVIDTLVGAGANDIGGINFTVSQASKLL
                     DDAREQAIADARRKAEIYAKATGVTLGAPLSISEGSTPVPIPYRRMAAGMAAAASVAQ
                     GEETLQVTVAVSWAIKQAGQ"
     gene            complement(5400..6464)
                     /locus_tag="VN968_26105"
     CDS             complement(5400..6464)
                     /locus_tag="VN968_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL32740.1"
                     /translation="MPITRRRVLGVLAGAAAIIGLPSVWISRMKTYSGQVSDHFDGTR
                     FLDPDGSPPKSLGEVLRWQFGPGRQRQAWPEWAPSGHADRPPARVWGDKARLSFVGHA
                     SWLIQTSGLNILIDPVWSARASPVNWAGPKRCNDPGIAFEALPPVDIVLVSHGHYDHL
                     DVATLSKLAAKFSPRVITPLGNDVTMRSADEAIRAQAFDWHDRVELGNGIAVTLVPTR
                     HWSARGLFDRNKALWASFVVETPAGKIYMVCDSGYGDGKHFRRVAQAHGPLRLAILPI
                     GAYEPRWFMQDQHMNPSDAVKALADCGAGQALAHHHGTFQLTDEAIDAPAIALGAALD
                     EAKLPRERFVTLKPGQVVEI"
BASE COUNT         1324 a         2273 c         2157 g         1174 t
ORIGIN      
        1 aaatcggcgc gcagcagcac atcctcgata tcgtcgagca tcgcgcgatc gagcttgcgc
       61 ttggtgacga gatcggccac cgccgacccg agcgagctcg aggtgcgctt caggccgccg
      121 gtcagccgtt gccaccagct cggttttgtc gttgcggaag tggtatcatt catgccgggt
      181 gtgttagcgg tttcgtgcca tgagcgaaag tctcgaccga ccggccgaag ttcccgaatt
      241 gacagcggac gaaatccagg cccgcgtgct ccatcgcgac gggctgatgg tggtgatcga
      301 caagcccgcc gggctgccgg tgcatcgtgg ccccaaaggc ggtgccaatc tcgaatcctc
      361 cttcgacgcg ttgcgcttcg gcctgccgcg gccaccggtg ttggcccacc ggctcgacaa
      421 ggatacttcc ggctgcctgg tgctggggcg ccatcgcaag gccaccgcct cgctcggcct
      481 gttgttcaag catggcagga tcggcaagac ctattgggcc gtcgtggaag gcggacctgc
      541 tgaggatgaa ggcaccatcg acatgccgct cgggcggctg aacgtcgagc gcggctggtg
      601 gcagaagccg gatcctgacg gtcaaaaagc cgtcacaaac tggagggttc gcggccgcgg
      661 caacgggctc gcctggcttg atctggagcc ggtcaccggc cgcacccatc aactgcgcgt
      721 tcacacgtcg gcagcgggct ggccgatcgt cggcgacaac atctacggca gcggcccccg
      781 cttcggcgag ccgcgcctgc atctgcattc ccgcgagatc gtgattccga tttcgaagaa
      841 caaggaaccg gtccgcatcg tggcacccgc gccggagcac atgcgcgaac ggctcaggac
      901 gtgtggctgg aacggagagt agttggcaga ctccagcgct tgatcccgac gacgggcacg
      961 aactcgacca aacttgcggt ttcgaacatg cccggcagtg accgccaatc cgacgcaaga
     1021 aaatctcgcg cactgttcat ctcagcatcg gacatgcccg tagccagggt gcagtggggc
     1081 gtccagcttc cagttcgata gtagggccgg caggtttcgc cgatcgcagc gacggtcgcc
     1141 gcatgtaacc gcatgagatc aagcgaaggg gccagggcca cgtaacaaac gccgctgcca
     1201 gttccgaacg tcgagaaatc ggttaaggtg acgggtatct gaccgacgtt atcaaaagct
     1261 cgatcaagcg atgcgactgc cgcgtccacg gctaacgcgt cataaacggc caacgtcaca
     1321 tgtggcggat aattgagatc gagcatcgac ttagataggc ctgcatccgc aagccgcgtc
     1381 cagcgcgccg aaactgcatc agctatttca ggattgagaa ggaggcttag cgcgaaggcc
     1441 atgcttggct agcttcctgt taaccgcgca ccatcatgtc cgaccatcgt caatgcccgc
     1501 accgcacccg gcgtttcgcc tgatatcgcc accggcaaaa agtgttcggt ccgcccttgc
     1561 ccggcgctct cgatcagaac ctgacgcgtc gcgccgattt ccgatgtcag ccgccgccgc
     1621 aacgccgcct cggctgccgt gcgcaatcgc ttcgcccgat ccttgatccg ttcgcgcgac
     1681 acctgcggca tccgggccgc cggcgtgccc ggccgcggcg aatagggaaa cacgtgcagg
     1741 aaggtaagat tgcattcctc gaccagatcc agcgagcgcg taaacatttc cccagtttcg
     1801 gtcggaaagc ccgcgatgat atcggcgccg agcgtgatgt ccggccgcag ccgccgcacc
     1861 tgtgcgcaaa actgaattgc ctgcgcgcgc gaatgccgcc gcttcatccg cttcagtatc
     1921 aggtcgtcgc cggactgcag cgacaaatgc agatgcggca tcaaccgttc gtcatccgcg
     1981 atgacgtcga gcaggtcgcg atcgacctcg acctgatcga tcgatgaaat ccgcaagcgc
     2041 ttcaattccg gaacgtgccg caagatctgt tttgtcaatt tgccgagttt cggcgcgccc
     2101 gggaggtcgg cgccataact cgtcagatca accccggtca gcacgatctc ggcgtggccg
     2161 cgctcgacca gcgcgcggac ctggctgaca acggcgccca tcggcaccga gcgcgaatga
     2221 ccgcggccga aaggaatgat gcagaaggta cagcgatgat cgcagccgtt ctgcacctgc
     2281 acgaataccc gcggcaggcc tttctgaaat ccgtcgacga gatgcggcgc cagttcgcgg
     2341 accgccatga tgtcggcgac cgcgatcttc ccgctcgctg cgatcccgaa ccccgcaccg
     2401 gcgtcgagcg ccgtgcgggc cgcgcgccaa gcttcccccc gcatcttgtc gtcattgccg
     2461 acgacgcgat cgacctcgac catatcggcg aacatccggg gctcgatctg cgccgcgcaa
     2521 ccggtcacga cgatacgcag ttccggccgc tcgcgcttca gccgccggat cgattgccgc
     2581 gcctgcgcca cggcttcgtt ggtgaccgcg cagctattga tgacgacggt gtcactgagg
     2641 cctgcgctct ctgcctcgcg caaaatcacc tcggattcga aggcgttgag gcggcagccg
     2701 aaggtgacga tttcgacgct catgatctac gcgacggaag caaacagcgc cggatcgaag
     2761 cggccctcat actcgaaggt cgccgtgccc gtcatcaaga cgtggtcatc cccctcgcgc
     2821 cactcgatca agagctcgcc gccgggcagc gagatctgca ccctgcgatc ggcgcgcttg
     2881 agccgcgccg ccgccacggc agtggcgcag gccgccgagc cgcaagcctt ggtcagcccg
     2941 gcgccgcgct cccaggtgcg gatcgcgatg tggccgcggt cgacgatatg cgcgagcgtg
     3001 atgttggcgc gttcgggaaa gatcggatga ttttccagga gcggcccgaa gcgggcgaga
     3061 tcgtaggcgt tgacgtcctc gacccagaag atcgcatgcg gattgcccat gctgaccacc
     3121 gaaggtgtgt gcagcaccgg cgcgtcgatt gggccgatct gcagctcgat cgaacgggtg
     3181 tcgcgaaact cctccgccag cggaatatcc ttccagccga atttcggcgg gcccatgtcg
     3241 acagtataga gatcgccgga tggtccctgc cagcaatcga ggagcccggc ccgggtctcg
     3301 aaggtagcgg cggtttggcc cgtgtcctcg aacaaacggc gtgccacgca gcgcatgccg
     3361 ttgccgcagg cggccgcttc ggagccgtca ttgttgtaga tgcggatgaa ggcctcggtg
     3421 cccttgaggc gcggcggctg cagcaccatc agctggtcgt aatgcacgcc gccaggggta
     3481 gctatcgcgc gggcttcctc tgacgtgatg acggcggact tgtcgcgcat atcgaccacg
     3541 acgatctcgt tgccgatacc gttcatcttg gcaaacgctt ggttggcgag cgcgctcatg
     3601 gaatttcccg attgtgcgcc ccttatatgg ccaatgatgc cggattggcc agtgcggcaa
     3661 atctgtgtcg gctaccttcg ctggacagag aaggcccatg acatatctgg gatgggacga
     3721 aagccgactt tgcgtgttag cctgcatctg ccgcctgcgg aatccgggaa gacgctgcgc
     3781 aacgccattg cccaaaagac catggagtcc gtccgaatgc atcgctgccg cttttttcgt
     3841 cttgccatgg tcgcgctggt cccggcggcc gcgatttggc tggggcttgg caacgtgctg
     3901 gcacaaacgc cgccttccgc tcccccggcc gtcagcacgc caacgccacc ggcgcctgcc
     3961 ccgtccgcaa cccccgcccc ggcacagccg gcaaccccgg ccccgggagc cagttccccg
     4021 gccgcgcctg gcacgccgcc tgcaacggct gagaccccgc cgaccctccc gccggcaacc
     4081 gcgccggtcc agaccgcaga tccgttcggc gaagaaatca cgctgacgcc gaaaacggtt
     4141 gtcgtcatga ggggcaatgc caattgggat tcggcgttcg agaccctgat cgattcgttc
     4201 aaggcgctgt cggcattgct cgacaagcaa ggcatcaagc cggccggcaa ttcgatgatc
     4261 gtctacaccg ctaccgacga caccggcttc acgtaccttg cggaaatacc ggtcgagcag
     4321 gagccaaaga atctgggtaa ggatatgagc atcggcaaat cgcccgaggg caaggcgctg
     4381 aagttcgtcc atcgcggctc ctacgacaat atggacaaca cctatgaggc catcaccaat
     4441 cacctcgacg acaagaagct tgaggccaag gacacctttg tcgaggaata cgtcaccgat
     4501 cccctgaaga ccgcggagga caagctggtg atcaacgtct atgtgccgct gaaatgaagc
     4561 tcctgaagca tcgcgctccc ctgttcgccg ccatcgccgc gggcacgctg cttgccgcgc
     4621 ccgcgctggc gcagaccgcg ccgcctgcaa tgatctccgt cgccggcgaa gccaccgtat
     4681 cggtgccgcc cgatcaggcg cagatcgacg ccggcgtcgc caccgaggcc aaaaccgcgc
     4741 gcgaggcgtc tgacgccaac aacgcggcga tgggtaaggt gctgctggcg ttgaacggcg
     4801 ccggcatcga ggagaaggat tatcagacct cgcggctgtc gctgcagccg gaaagcgcgc
     4861 cgaaccgcag cagcggcccg tcgaccatcg tcggctaccg tgccagcaat cgcgtcacca
     4921 tccgccttcg cgatgtcacc aaggtggcaa gcgtcatcga tacgctggtg ggcgccggcg
     4981 ccaacgatat cggcggcatc aatttcacag tgtcccaggc gtcaaaactg ctcgatgacg
     5041 cccgcgaaca ggcgatcgcc gatgcccgcc gcaaggccga aatatacgcc aaggccacgg
     5101 gcgtcacgct cggagcgccg ctcagcatct cggagggcag tacgcctgtc ccgataccct
     5161 atcgcaggat ggccgccggc atggccgcgg cagcatccgt ggcccagggc gaggagacac
     5221 tgcaggtgac ggtagccgta tcctgggcga tcaagcaggc gggacagtga tgttttttag
     5281 ccgagcgctt cacctcatcc tgagaagcgc gcactcggcg cgcgtctcga aggatggaga
     5341 cgggccgtgc ttcgagcgcc aaagcgcctc ctcagcatga gggattgcaa acgagcgctc
     5401 taaatctcca ccacctgccc cggcttgagc gtgacaaaac gctcgcgcgg caacttcgct
     5461 tcatcgagcg ccgcgcccag cgcaatggca ggcgcgtcga tcgcctcgtc ggtcagttga
     5521 aacgtgccgt gatgatgcgc cagcgcctgt ccggcgccgc aatcggccag cgccttgacc
     5581 gcatccgacg ggttcatgtg ctgatcctgc atgaaccagc gcggctcata ggcgccgatg
     5641 ggaaggatcg ccaaccgcaa cggaccatgt gcctgcgcca cgcgacggaa atgcttgccg
     5701 tcgccatagc cggaatcgca gaccatgtag attttgccgg cgggagtctc cacgacgaaa
     5761 ctcgcccaca gcgccttgtt gcggtcgaac agtccgcgcg ccgaccaatg ccgagttggc
     5821 accagcgtca ccgcgatgcc gttgccaagt tcaacgcggt cgtgccagtc gaacgcttgg
     5881 gcgcggatcg cttcatcggc gctgcgcatg gtgacatcgt tgccgagcgg ggtgatcacg
     5941 cgtggcgaga atttcgcggc gagttttgac agcgtcgcga cgtcgagatg atcgtaatga
     6001 ccgtgcgaca ccagtacgat gtcgaccgga ggcagcgcct cgaacgcaat gcccgggtcg
     6061 ttgcagcgct tcggtccggc ccagttcacc ggcgacgccc gcgccgacca caccggatcg
     6121 atcaggatgt tcagtccgga cgtctggatc agccagctgg catggccgac gaacgacaat
     6181 cgcgccttgt cgccccagac gcgggccggc ggcctgtcgg catgcccgct cggcgcccat
     6241 tcgggccagg cctgccgctg cctgcccgga ccgaactgcc agcgcaagac ttcgccgagc
     6301 gatttcggcg gcgagccgtc gggatcgaga aagcgcgtgc cgtcgaaatg atcggacacc
     6361 tgtccggagt aggtcttcat tctgctaatc cagaccgatg gcaggccgat gattgcggcc
     6421 gcgccagcca gtactccaag aacacgacgg cgggtaatgg gcatggcccg attggcccca
     6481 acaacgcgca tttcggcaag gggctaatcg gtcgtcccgc ttcacgaaaa tgccattttg
     6541 ccgcgagttt ccttgacttt ggggggtgag aggggtttaa cgcccgcaat catcggaaag
     6601 acgttctttt cgacccgccg cccggtcctt gaggccggag gccaacgccc gacagcgcga
     6661 tgcgccctcg ggcgcaaaag tgtttggatt atcgtcgtcg cagcgttgcc gaggggcgaa
     6721 gcgagacaca ggttaaggtg tcatcacccg cgaaagcggg tgatctggta accgagagcg
     6781 cccgttccaa ggcgaggcct cggagtactg gataccccgc cttcgcgggg tatgacggca
     6841 gtcaaactaa cgacctcatc ctgaggagcc gcgaagcggc gtctcgaagg atgggccacg
     6901 aggaacaatc cacccttcga gacgcagg
//
LOCUS       DATNOI010000649        26736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1678026,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000649 DATNOI010000000
VERSION     DATNOI010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 26736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26736
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1678026"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            834..1211
                     /locus_tag="VN968_26110"
     CDS             834..1211
                     /locus_tag="VN968_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 domain-containing protein"
                     /protein_id="HXL32741.1"
                     /translation="MRRRLEVFIPIVLLSILVQLFAPIAAFRVVAYAVTDPVYMASIC
                     TEMVSPPDAQTAPAKTQHDHGDCCVFCAAGHGGTIAVEPPPPIFVSLQRQYQRVSWLE
                     AADPISAGRVGSNAQARAPPQLT"
     gene            1270..2271
                     /locus_tag="VN968_26115"
     CDS             1270..2271
                     /locus_tag="VN968_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32742.1"
                     /translation="MSIRLWRAGLSGLAIALVPLSQSVAHEIVGNRFFPATIGIDDPG
                     VNDELSLPTVDNFKTGDVPPVRQRDISGEFSERITEDFAISFGSTYTFLGPIDPTAAG
                     ANGFQNLDTTFKYRVFKSPEHEFVMSVGLSVEWGGTGAANVGAEAFNTYVPNIYFGKG
                     LGDLPDTLSWIRPVAITGQVGYAIPARNFTTTFGIDPDTGNQTVDTEFHPRVLNWGGT
                     IQYSMPYLKSAVIDLGLPEFINHLIPLVEATMQTPVANTLTSGTMTTGTINPGVIWVG
                     STFQVAVEALIPINRQSGTNVGVIAQLHLYLDDIDPRGIGKPIFGGPVQPASPFARN"
     gene            2274..2630
                     /locus_tag="VN968_26120"
     CDS             2274..2630
                     /locus_tag="VN968_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HXL32743.1"
                     /translation="MHRLSLARMVPLLLLLATGEASAHAFLDHAEPRVGNNVATPPRE
                     VTLWFTQNLEAAFSTITVTNPAGQRVDTGKPRVSGNKMSVSLRSGGAGTYHVTWRVLS
                     VDTHSTDGSFTFQVGQ"
     gene            2712..3206
                     /locus_tag="VN968_26125"
     CDS             2712..3206
                     /locus_tag="VN968_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HXL32744.1"
                     /translation="MLVYFIVAPALAEEVKAGYLVITQAWSRATPGGAKVAGGYLTID
                     NKGSTPDRLIGGSADVADKVQAHQMTMNNGVMTMRPLDQGLTIAPGKTVKLAPSGYHL
                     MLLDLKSPLKRGDKVPVILEFEKAGKVTVSFDVQGVGAPAPATAPSSGSKKDMKGMTP
                     GMKM"
     gene            complement(3436..3813)
                     /locus_tag="VN968_26130"
     CDS             complement(3436..3813)
                     /locus_tag="VN968_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXL32745.1"
                     /translation="MTAESIDYDSLMQANLARVFGERDASRRMKAIAELYADDAILYE
                     PDAAATGHAAISQAVKTLLSSLPPNFVFTAIGPAVGHHGLGRLRWQSGPPNSPAAVTG
                     TDVARIEGGRIRTLHVFIDPAGA"
     gene            complement(3974..4855)
                     /locus_tag="VN968_26135"
     CDS             complement(3974..4855)
                     /locus_tag="VN968_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL32746.1"
                     /translation="MQTRADRLTPLPENVLSLSVARRAWNGVGVDVSEFHCVGRVAHH
                     LGYESETRLSVLLEEVGSHCEPRLREDQPCPIGYIPRHMQFAPAGMDMWGYSADTRFV
                     KDATLAFDLAVLGERLTTEFDADATTTPRLRFSDDRLWTLVRLLSDAVNDPDPSTQLY
                     GDGLTAAIAARLFAEPLGPGADAKGLAPGQLQRVVEYLDARLPKRVDLAHLAALAGLS
                     QSHFSRAFKASTGMAPYRWQLDARIRRAQALLIGTSASLDDVAEATGFADAVHFGRTF
                     RKLTGATPAAWRHDRKS"
     gene            5298..6095
                     /locus_tag="VN968_26140"
     CDS             5298..6095
                     /locus_tag="VN968_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32747.1"
                     /translation="MDSTPNAKTTEPDDDLIARADERLAHAYEEIARADEQIARVNEQ
                     VSKLEHDAARNPSAALGRLPSRGRPMLRGLIGLLLAACIFITAFVSQSSHGDAAKLIF
                     ARWAPQLILTSSLPMENPGLPARPSPSAVQVAAAEPVAAQPTPLAPTAPRDVAPTAAP
                     VSPEVAQLLQTMARDLANVEQRIEQLKASQEQMATDNARAVEQLKASQEQMRRLIAKA
                     SEQNLRPKTSAPPPQLTASPTRKPVPKRPSPQARAQPQAPVQLQPDE"
     gene            complement(6137..6272)
                     /locus_tag="VN968_26145"
                     /pseudo
     CDS             complement(6137..6272)
                     /locus_tag="VN968_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654629.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
     gene            complement(6433..6609)
                     /locus_tag="VN968_26150"
     CDS             complement(6433..6609)
                     /locus_tag="VN968_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXL32748.1"
                     /translation="MVFGAHSLRRTNATRICRRNANLRAGQPLLGHTKIEGTVRYLGI
                     EVDDALAMAEQVDV"
     gene            complement(6619..7077)
                     /locus_tag="VN968_26155"
     CDS             complement(6619..7077)
                     /locus_tag="VN968_26155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32749.1"
                     /translation="MKEVFDQLLKFLQQGISAIFRFVELIWTWSVDQISKLMAVPWQE
                     WPLLKQVLLLLILAGVLWALYRAGWALLEAGAAILAAFAGLLGVLVQTLPHVFLAGVI
                     ALGGVWLVNHLDNSSLRMPSWTQASEQNSSPQNSSPPKSPSSTPQGQPSQ"
     gene            7378..8307
                     /locus_tag="VN968_26160"
     CDS             7378..8307
                     /locus_tag="VN968_26160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HXL32750.1"
                     /translation="MSIALRLSPLAACKSPLLQVAVAAIRGWRSLSYQAEISRTSPTA
                     IVMVMDQSASMNHRLKSGQSKAAFLADVLNKTLYTLITTCSKADGVRDYFHVGVVAYG
                     GEDARNGFQGALDGEALQAVSRLAEAPLRVETRAKKIAGPNGEVAEQSVRFPIWFEPR
                     SQGKTAMCAGLNAAIGILEQWRDAHPAAFPPTVLHVTDGHPSDGNPEPIADRLKSLAT
                     DDGACLLFNLHVDAGDGEPLIFPNDERVIQDRFGKSLFRMSSLLPPHALEAAAAKGYD
                     VRPGARGFVFNAGIEAIADFFDIGTRPAIAADR"
     gene            8332..9171
                     /locus_tag="VN968_26165"
     CDS             8332..9171
                     /locus_tag="VN968_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015273089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HXL32751.1"
                     /translation="MPYQAEISRDNPTCFLFVIDQSGSMDEITETGRSKADFVADVLN
                     KTLYTLITTCSKAEGVRSYFDVGVIAYGGKEVASGFGGALSGGIVHPINAISEHPLRV
                     EERQKKVDDGAGGILELKTKFPIWFDPKSVGGTPMRAALDKTRETVAEWCEAHPASYP
                     PTVLHVTDGQSTDGTPEEMADALRRIATKDGQALLFNLHVTSQGGPEILFPTSETGLN
                     DEYSRMLLRMSSPLPPHLAEFADDKGYAIADGSRGFIFNGDPQCIVDFFEIGTRPKLV
                     ADR"
     gene            9168..9902
                     /locus_tag="VN968_26170"
     CDS             9168..9902
                     /locus_tag="VN968_26170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32752.1"
                     /translation="MSTPELRQKSRLPLALSIPKTEDSRNEDGFYRSAKGVYALSDGA
                     SVSFDSASWARILVRHYTRNPQFTREWLAGAIAEFRKLYDRETLPWMQQASYDRGSFA
                     TLLGVRIVDEGRLIQIFAIGDTLAVLCDGDRIKATFPFSAASAFTRSPKLLCTNPVGN
                     AFLDEVDFGYDLVADWTFTGLKQPALLCMTDALGHWLLSQRDRNSSPISALRKLQTPR
                     AFARFVHEERAAGRMKRDDTTLIALW"
     gene            9910..11538
                     /locus_tag="VN968_26175"
     CDS             9910..11538
                     /locus_tag="VN968_26175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HXL32753.1"
                     /translation="MSYPQITDYQEAVQHPAQAFIDPDLKQGAVAENNLGLPLVMSGG
                     FALTYAVTTPRRKCAVRCFHREIPAIQQKYDATSRKLRSLAIGCFVDFDFQQSGISVR
                     QRVFPIVRMDWVEGDTLGIWLDKNFNNPRALEKARADFAAIARFLEREGIAHGDIQNG
                     NVMVANGEIKLIDYDGMFVPGMHPGNGSETGHRHFQHPGRRVSNFGPGMDRFSFIALD
                     LSLRALVEDRSLYSKFREGGETIIFKANDFADPQNSEIFRRLLAMPKLKAQAGSFAAI
                     CEASLAAVPTLDEFLSGRNIPAAKTPINPTTTPATKPAAAYIPAYPVVDALNFSAALQ
                     RVGDRVELIGRIVEIKPGSRKQGKGPNKRYVFINFGSWSGNIVKISIWSDGLARLKDK
                     PSAAWVGRWVSVTGLMDVHETKRYRHLSITVQEDGQIQWLDESQAGFRLASIVMAPPP
                     RDRVSTGAASSGSPVAAPPAAAAKPKTLRPRSQGTARGHKRAGRQQPIPAATIAVPTQ
                     QGTQSLGRFLAAIPLWMLGIGTLIVLIMLLRMYA"
     gene            complement(11817..12269)
                     /gene="nikR"
                     /locus_tag="VN968_26180"
     CDS             complement(11817..12269)
                     /gene="nikR"
                     /locus_tag="VN968_26180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HXL32754.1"
                     /translation="MQRITITLDDDLMEELDTMIAEHGYQNRSEAIRDFARAGMQQAA
                     HAKGKSGECVAALVYVYDHAARDLSRRLVENYHGHHDLSLATLHVHLDDDNCMEVTAL
                     RGPGAEVQHFADHVIAERGVKYGRVVMIPTETGKKKDVPRKRIGHRHS"
     gene            12417..14726
                     /locus_tag="VN968_26185"
     CDS             12417..14726
                     /locus_tag="VN968_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXL32755.1"
                     /translation="MHRSSRGLAAIWLVGWVAVSPIVSAQDAKPIPLPAVQIDAPQQR
                     QDARFKRKHSADRGGRSNRTAIQNPVSVANAAPAQSALGASAAAAPVASSSEKNVSGE
                     EVNARPFSRPAEALEVVPGLIITQHSGDGKANQYFLRGFNLDHGTDLAISVDDMPVNM
                     RTHAHGQGYADLNFLIPELIGSVNIRKGPYFADEGDFSSVGSVHVGLLDNVNKTMASI
                     TAGSFGYRRILGITSTKLGDGTLLIAGEANTYNGPWDNPDSLRKLNGVMRYGQGTAED
                     GFSLTGMAYANKWNSTDQIPSRAIASGEIGLYGAFDPSDGGNSNRFSLSGRWAQTDEG
                     GTSKANFYVIKSSLNLWNNFTYFLSDPVNSDQFHQHDDRVLGGINGSHTFKSQFAGLP
                     METEIGVQTRYDDIRLDLNNSVQRQFLSPIRSDAVQEGSVGVFAQNTLHWTDWLRTNV
                     GWRGDFYQTSVNSFFTPANSGSANAFIGSPKFGAVFGPFAKTEFFINAGEGFHSNDAR
                     GVTITESPGDGSALQPSPFLVKTRGAEVGVRSKLVPGLTSSMSVFLLDSASEILFSGD
                     AGDTRPSRPSRRYGLEWTNDYRPLSWLSLEGDIAVTHARFRGDDPPQAALYASLAGFP
                     AAQIGNAPGNYIPGAPSMIASAGIRLGERTGWFAGLRYRYFGPRPLTEDNAFVSPATG
                     LLNGQLGYRFDNGWRVQLDAFNLLDSRSDQITYAYGSLLKSDSLFAMCFPAAGAPTAP
                     AAVCQTGVMDGVLHPVEPLAVRVTVAGQF"
     gene            15038..15850
                     /locus_tag="VN968_26190"
     CDS             15038..15850
                     /locus_tag="VN968_26190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32756.1"
                     /translation="MIAPDIVLVARADKAHDAVSRHSDSQTHMAPDFSAGPSVLPVDT
                     TFRAAAVDDVQVRGDGRSIGGRVIRACIGFLLAVCLAVAASLWPSYGDAAKQIVARWV
                     PQFALTSSSPQDNPGLPEQPGPAAVQASAAKAEPPAQTAPESVASTSATLSPESTQLL
                     QSMAGDLAHAQQEIEQLKASIAELKASQQQMSRDTAKVSEARVSGARIAEAKVSEQNL
                     RSRLSAAVRRSASARKPMPPLPPPQAAAAPPLPQATTQPQAELVPRPPMPLR"
     gene            16076..16372
                     /locus_tag="VN968_26195"
     CDS             16076..16372
                     /locus_tag="VN968_26195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HXL32757.1"
                     /translation="MKIAKIMLAGTAALTIIGSAALAQQALTGTVTKVDRINRSVAIK
                     QTQTGTVGANTGGAAEEFKAQDGLSLDTLHAGDTVSFSATETGGVKTITKLQKQ"
     gene            complement(16456..17652)
                     /locus_tag="VN968_26200"
     CDS             complement(16456..17652)
                     /locus_tag="VN968_26200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HXL32758.1"
                     /translation="MSAPVTDSVELSRPMLFQPFEIRGLTLKNRLVVPPMVHYRAGPG
                     NTCGSFHHVHLGRYALGGFGLVFVEATAVEEVGLINEHDLGIWNDAQVQSFRPLIAFM
                     KGEKAAIGIQLAHGGRKSSSQRAMDGMGPLTPQEIKAGAKVWQPVGPTSEPVANGWLT
                     PHQLTTEECRAMVRTWAEAARRAVDAGFDVIEIHTAHGYLLASFLSPVSNTRHDEYGG
                     DRNGRMRLPLEIVEAVRREMPATMPLFVRVSAVDGAQNGWNLDDTVIFARELKARGVD
                     LIDCSSGGISGSATAAQVPRGLGFQVPYAERVRKDVGIASMAVGIILEAQQAESILEN
                     GQADLIAVGRQSQFNPNIAQHWAHDLGINRKFEDWTPEFGWWLEKRIRTIEGFATPTG
                     VVKRHA"
     gene            17932..18855
                     /locus_tag="VN968_26205"
     CDS             17932..18855
                     /locus_tag="VN968_26205"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXL32759.1"
                     /translation="MRIAVVGAGGVGGGFGAALAKAGADVIFIARGAHLAAMKREGLK
                     VQGGRGETHLVPTQATDDPASIGKVDIVLFCVKLWDVENAGQKIRPLIGPDTAVIPLQ
                     NGIDAAERLIPILGPDAVMGGVAQISASIVEPGLIQQVGSFMRMIFGELDGKRRKRGE
                     DFLALCLQAGFDATLSEQILTELWMKFILLAGNASIMALARQPIGRLRDDPDLRPIFI
                     AAYRETIDIGRARGVALPADALEKILDFTGHAPPAMKPSMALDLERGNRLELPWLGGK
                     VVELGLELGVPAPTHSMMYAMLKPYIMGTPA"
     gene            19414..19740
                     /locus_tag="VN968_26210"
     CDS             19414..19740
                     /locus_tag="VN968_26210"
                     /inference="COORDINATES: protein motif:HMM:NF019370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HXL32760.1"
                     /translation="MSWDAEDVALLKKLWAVGRSAAQIARRLGYSRNAVCAKLTRLGL
                     KRGHKPPTAKPRIRPVPKRRPALLAACASPVAKKVSRKTAMPRPKEFTKRQLYAMLAE
                     AVRNTG"
     gene            19877..20161
                     /locus_tag="VN968_26215"
     CDS             19877..20161
                     /locus_tag="VN968_26215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32761.1"
                     /translation="MALLKRDLYRQVEGPEVTHADRCTLVFDTDSKNLYVEREVAHLD
                     AKVGGTVDYQTATMDIADYLKQGGQTAGHRELWRLLRSIFKLDSDADQAI"
     gene            20589..21035
                     /locus_tag="VN968_26220"
     CDS             20589..21035
                     /locus_tag="VN968_26220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32762.1"
                     /translation="MSGRALTGVAVLAALAVSNPITTAQTVPKLDYEFFKSRVEPVFL
                     TKRPDHARCYVCHVESNNAFHLEKLAPGARAWTEEQSRRNFEMVSVLVNPGDPDTSRL
                     LLHPLGPEGGGDVFHSGGRQFASKRDPAWRMLAAWVNGATLASQKK"
     gene            complement(21001..22098)
                     /locus_tag="VN968_26225"
     CDS             complement(21001..22098)
                     /locus_tag="VN968_26225"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HXL32763.1"
                     /translation="MNVYRFLRHIWILLMVAPVPAWAGTVRVYVTNSAGDSIHVIDPA
                     TNKVVQQIKGIEGAHGIAFSPDGSKVYVSDEVDSTLDVFDRKSGALVKKVALSAHPNN
                     IAVARDGRIVVGIARDPGALDIIDPATLTRTKSVPVNGRLHNVYVTPDSKYVVTGSIR
                     TGIVTVIDLATEQPAWEVRLDKGIRPMAIEAGPDGSTKRIFVQLSDFNGFAVVDFAAR
                     KEVARVQLPAAKTEFETDGDRATAPSHGIAVAPDGKSLWVTSIPNNAVYVYSLADLAL
                     AGEVALPSLTLPGHGSISAVANWVTFTPDSKTAYISNAGLRSVSAIDTKSMKLIAVVP
                     VGEVPKRINTLVIPDHPGSAAISSEKRASLH"
     gene            complement(22148..22771)
                     /locus_tag="VN968_26230"
     CDS             complement(22148..22771)
                     /locus_tag="VN968_26230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32764.1"
                     /translation="MSIHRRLARAAFGALLIPIALGCAPVYADGAPVRVRGAVVSLDG
                     SKLVVRAKDGKDVSVSLADNFAALAVVKSSMADIKEGTFIGTATVTQPDTTLRSVEVV
                     VFPDSLRGTAEGHYPWDLGSSSMMTNATVANAVKAVDGQTVTVTYKGGEKKIDIPANV
                     PVVALVPASKDDIAPGAIVFVPTERQSDGSLHSGAVLFGKDGVIPPM"
     gene            22770..23129
                     /locus_tag="VN968_26235"
     CDS             22770..23129
                     /locus_tag="VN968_26235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32765.1"
                     /translation="MKHLLDGRGGGNVARWRKTVSVGFLSGITIPRCRDRQEAKRRRP
                     GGLFLASPAVAALQAIDGDNKVGPLEHFDQPVKDALLVVRARLQIFFEDALSLAYGLK
                     RQLLIGHNFSLNAEKQT"
     gene            complement(23331..23540)
                     /locus_tag="VN968_26240"
     CDS             complement(23331..23540)
                     /locus_tag="VN968_26240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32766.1"
                     /translation="MNHSIYSADRLTHLKIVMVALCAAIVVAGLALSFRDTSGDGSAH
                     AERIIKAGKPVMETNSVGAVVRWRL"
     gene            24023..25621
                     /locus_tag="VN968_26245"
     CDS             24023..25621
                     /locus_tag="VN968_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXL32767.1"
                     /translation="MIEERCTRRPPYQGETQGLRRVQQTNPEGIATVDGDRRRWREIG
                     ERVARLAGGLRELGVNRGDRVAVLMLNSDRYLELYLGIAWAGAVIVPTNIRWSHAEIE
                     DSLHDCRASVLVVDKAFARMGVELAKAVPLALIYADDDAGPAEAQNYEKLIAASEPVP
                     DAMSTREELAGIFYTGGTTGRSKGVMLSHANIVSNALHMLSEGLLPDGTIYLNAAPMF
                     HLANGGAMFASLIGGGTNVIVRTFNAELVMDTIERDKVTATLIVPTMIQMLVDHPAFQ
                     TADLSSLQRIMYGASPISEALLNRAMWGLPGTAFHQLYGMTELSPLATHLPWDQHTGE
                     AASKKNRQRACGRAAIGCEVRIVDAARKPVASGVVGEVAVRGQNVMMGYWERPEETAK
                     AVIDGWMHTGDGGYMDEEGYVYLVDRVKDMIISGGENVYSMEVENAIAQHPAVSQCAV
                     IGIPSEQWGETVHAFVIPKAGAHVNAAEIIAFCKERIAGYKCPRSVDVRTEPFPLSGA
                     GKVLKRELRRLYGQDSALAQAKAG"
     gene            complement(25623..26417)
                     /locus_tag="VN968_26250"
     CDS             complement(25623..26417)
                     /locus_tag="VN968_26250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXL32768.1"
                     /translation="MTEAVAPRPASTILLLRDGAGAEIEVFMMVRHYEIDFNSGALVF
                     PGGSVDKGDQDIIASPELYCGGEGLDTASLSFRIAAIRETFEESGILLARPRESKALV
                     DARRASAIEADHRAALCEGKTTFLNVLTDNGMVLALDELVPYAHWITPEGLPKRFDTW
                     FFLAAAPPEQVGAHDGKESTDSIWVSPREALEGGGSGRFKLPFPTTRNLIRLGKQPNV
                     KAALDDSMGKPIVTVMPVVTKRDGVRQLRIPLEAGYDGEAFEVGAA"
     gene            complement(26422..>26736)
                     /locus_tag="VN968_26255"
     CDS             complement(26422..>26736)
                     /locus_tag="VN968_26255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXL32769.1"
                     /translation="AMTGYAFPELLIDVVNLSKAGKRDAAHDLFDAHLPLIRYEQQLG
                     AGLAVRKYVLQKRGVIASSAQRKPGSSITATAKAEVDYLLSRVARVDKRANLQPQSSA
                     AE"
BASE COUNT         5300 a         8271 c         8080 g         5085 t
ORIGIN      
        1 tctctcgccc caaggagggc gtggggaatg ccgggcgccc gctgcacccg cggcctcgtg
       61 tgcactttgt attggtagaa cgcacacgag taacaacgag tacaccggca tcacccggca
      121 ttcccgcacg caatggtttt aacggcttat gtcgtgctct ccccggtgac cgggctcttt
      181 tgccaccgtc gtccgcagag caaggttttg tcactgcccg gtcgggccaa caaacctccg
      241 cgaacttgac gccagcgtcg gggcgtcagg accacacgat tttgccgtcc gcaacaacat
      301 ctctcgtcag cgtgcagggg aatcgctcac aagtctttcg accagccctg cgatcccatc
      361 gcgcgccaaa cgctgccgcg tccaccgcat cccgccccta cgttcgtgac gatcatgata
      421 cgcccctctt ttggggtggg atgcgaaaag ttctagaggt gatttggggg gtgtgaaaac
      481 cgaaatattt ttgcaaaagg gactcgacac gcctgtcaac aaaccgcccg acgggcaaat
      541 caccacacga ctgcgaagag caatttcctc tcgtgtcccg gacgcggagc agcgtgctcc
      601 cggcgatgcg tagcatcgtc cggtcacgct gctccgcaga gccgggaccc gggccaaggc
      661 tcgcatcatt ttgccgcgac gaaacttcgt gaggcgaatt cgaatctatt ttgtgtgtcc
      721 catccgacac gatgggacgt ccgatgacgt cgagtcctga cgaacccccg cgagcaatct
      781 cgacagggag tcaaccgggg tgataggatc agcatggcgt tttgggggaa atcatgcgac
      841 ggcggctgga agtctttatt ccaatcgtgc tgctttcgat attggtgcaa ctatttgcgc
      901 caatcgcggc gtttcgcgtg gtcgcctacg ctgtaaccga tcccgtctat atggcgtcga
      961 tctgtacgga aatggtgtcc cctccggatg cgcagacggc cccggccaag actcagcatg
     1021 atcacggaga ttgctgcgtg ttttgtgcgg ccggtcacgg tggcaccatt gccgtcgagc
     1081 cgcctccgcc gatctttgta agtctgcaac gtcagtacca gcgcgtatca tggctggaag
     1141 ccgcagaccc aatatcggcc gggcgggttg gctcgaacgc ccaagcgcgc gcgcctccgc
     1201 agttgacgtg aaccgtggcg gccattggcc gtcgatgctg ttacagttca acattatcgg
     1261 agaatttcga tgtctattcg cctttggcgc gcgggccttt ccgggctcgc catagcgctc
     1321 gttcccctca gtcaaagcgt cgcccacgaa atcgtcggca atcgcttctt tcccgccacc
     1381 atcggcatcg acgatcccgg cgtcaacgat gaactgtcac ttccgacagt cgacaatttc
     1441 aagaccggcg acgttccgcc agtgcggcag cgcgatatat ccggggagtt ctccgaacgg
     1501 atcaccgagg atttcgcaat atcgttcggc tcgacttaca cctttctcgg tccgattgat
     1561 ccaacggctg cgggagccaa cggattccag aacctcgaca ccacgttcaa atatcgagtc
     1621 ttcaagagcc ccgaacatga gtttgtgatg tcggtcgggc tcagcgtcga gtggggcggc
     1681 accggtgctg cgaacgtcgg agcggaggcc ttcaacacct acgtgccgaa catctatttc
     1741 ggcaaagggc tcggtgatct tccggatacg ctatcgtgga tcaggccggt cgcaatcacc
     1801 ggccaggtcg gctatgcgat tcctgccagg aatttcacga cgaccttcgg catcgatccg
     1861 gataccggca atcaaaccgt cgatactgag ttccatccgc gcgtgctgaa ctggggggga
     1921 acgattcaat acagcatgcc ctatttgaag tccgcggtga tcgacctcgg cctgccggag
     1981 ttcatcaatc atctgatacc gctggttgag gcaacgatgc agacgccggt ggcgaatacc
     2041 ctcacctcgg gaacgatgac gacgggcacc atcaatcccg gcgtcatctg ggttggaagc
     2101 acctttcagg tcgccgtcga ggcgctgatc ccgatcaacc ggcaaagcgg caccaatgtc
     2161 ggggtgatcg ctcaattgca cctctacctc gacgacatcg atccgcgcgg catcggcaag
     2221 ccgatcttcg gcggccccgt gcaacccgca agcccctttg ccaggaattg atcatgcacc
     2281 gcttatccct tgccagaatg gtcccgttgc tgcttttgct cgcgacgggc gaagctagcg
     2341 cgcatgcttt tctcgatcat gccgaaccgc gcgtcggcaa caacgtcgcg accccgccac
     2401 gcgaggtgac actgtggttc acgcaaaacc tggaggcagc gttcagcacc atcaccgtca
     2461 ccaaccccgc aggtcagcgc gttgatacgg gcaaacctcg cgtcagcggc aataagatgt
     2521 cggtatcgct acgctccggg ggagccggaa cataccacgt gacgtggcgc gtgctttctg
     2581 tggacacaca tagcactgac ggcagtttta cgtttcaagt cggccagtag agccgcggcg
     2641 aggagcacga caaaatgcaa atcgaaaggc tggcgttcat accaatcaca cgcgcaatcg
     2701 cgtgtgccat tatgctcgtg tatttcatcg tcgcgcctgc gctcgccgag gaggtcaagg
     2761 ctggctatct cgtcatcacg caagcatgga gccgcgctac gccgggcggc gcgaaagttg
     2821 ctggcggata tctgaccatc gataacaagg gctccacgcc ggatcggcta atcggcggct
     2881 cggctgatgt cgctgataaa gtccaggccc accaaatgac catgaacaat ggcgtgatga
     2941 cgatgcgtcc gctcgaccaa ggcctaacga ttgcgcccgg caagaccgta aagcttgcgc
     3001 cgagcggata tcatctgatg cttctcgatc tcaagagccc gctcaagcgg ggcgacaagg
     3061 tgccggtcat cctcgagttc gaaaaggccg gcaaggtcac ggtctctttc gatgtgcagg
     3121 gcgtcggcgc gccggcgccg gctaccgccc ccagttctgg cagcaagaag gatatgaagg
     3181 gcatgactcc cgggatgaag atgtgagagt tttgggggcg atacccgtaa ggcagcaagg
     3241 agctggtctt cgcgcgtcgc cgtgcttgcc cagaggattg aatgatcttg aacggccgtc
     3301 gaccagccgc cgcgacaggt cgcgcgcggc acgatcgtag acgtagacca gcgcgatgct
     3361 gtcttccggg caaagcaggg cgtcgaggcc tgtcttggcg ggcatgatta ctccttggga
     3421 catgaaggcg gtggctcagg caccggcggg gtcgataaag acgtggaggg ttcggattcg
     3481 gccgccttca atccgggcga catcggttcc cgtcacggcc gcgggactgt tcggtgggcc
     3541 ggactgccag cgcagccgcc caagaccgtg gtggcccacg gccgggccga tcgcggtgaa
     3601 gacgaagttt ggcggcaagc tcgacaggag cgttttcacc gcctggctga tggcggcatg
     3661 acccgtcgcc gccgcatccg gttcatacag tatcgcatcg tcggcataga gctccgcgat
     3721 ggctttcatc cgacgcgagg cgtcgcgctc gccgaacacc cgcgcgaggt ttgcctgcat
     3781 gaggctgtca tagtcgatgg attcagcggt catgttctgc cttccggagg taaggagttc
     3841 cgcaaggcga gtgtcgtcac gcgctcaata tggttttgct cgcctgtccg gtcgagtgac
     3901 tattatccag ccgcctggcg aagacactgt tcgattctgc tgttggcagt ccgaatccgc
     3961 tatcgcgtcg tgatcagctc ttgcggtcgt gtcgccatgc cgccggcgtc gctccggtga
     4021 gtttccggaa cgtccgcccg aaatgtaccg catcggcaaa accggtggcc tcggccacat
     4081 cgtcgagcga ggcgctggtg ccgatcagca gcgcctgcgc gcgccggatg cgcgcgtcga
     4141 gctgccagcg atagggagcc atccccgtcg aagccttgaa cgcccggctg aagtgcgact
     4201 gcgacaggcc cgccagcgcc gccagatgcg cgaggtcaac gcgcttcggc aggcgcgcgt
     4261 ccagatactc gacgacgcgc tgaagctggc cgggagccaa gcccttggca tccgcgccag
     4321 gccccagtgg ctcggcgaag agccgcgccg cgatcgcagc ggtcagcccg tcgccataga
     4381 gttgcgtcga cggatcgggg tcattcaccg catcggacag cagcctgacc agggtccaga
     4441 gccggtcgtc ggaaaagcgc agccgcggcg tggtggtcgc gtcggcgtcg aattcggtgg
     4501 tcagccgctc gcccagcacg gcaagatcga aggccagggt ggcgtccttc acgaagcgcg
     4561 tgtcggcgct gtagccccac atatccatgc cggccggcgc gaattgcatg tgcctgggta
     4621 tatagccgat cggacagggc tggtcctcgc gcaggcgtgg ttcgcagtgg cttccgactt
     4681 cttccaagag aacgctgagg cgggtttcgg actcgtagcc cagatgatgg gccacccggc
     4741 ccacgcaatg gaattcactg acgtcgacgc ccacgccgtt ccaagcgcgc cgcgccaccg
     4801 acaggctcag cacgttctca ggcaatggcg tcaaacggtc ggcgcgtgtc tgcaagatcg
     4861 aactctccgg ctctcagacc aggcatccta gcgagatggc tggaaaaagg tagtgagcct
     4921 tggagcaacg aggaggtaag cgatgatgcc gcaaaaaaag taagacctgc accgtgatgt
     4981 ccgttgagcc cctgcgaatg ttttgacctc aagccgacgt tggaggggtc atgacaactg
     5041 atgatatcac cgatcgacag gcaacaggat cggactgttc gcgttgccga atgccgccgt
     5101 gcgctcctct ttaatacgcc cccgctacca agccgttggt gttctccctt agccgagttc
     5161 ccgaaggccg atggccacga gccgccctat attttccatc gtatggccat gtttcggtcg
     5221 aaactgcagt gtgtggcggt tcgtctacct agtgggaatc tgaccgcctc gttcgtgaca
     5281 ttggaaaagc agcgccaatg gattccacgc cgaacgcgaa aacaactgaa cccgacgacg
     5341 acttgatcgc gcgcgctgat gaacgcctcg cgcacgccta tgaagagatc gcgcgcgcgg
     5401 atgaacagat cgcgcgtgtg aatgaacagg tttcgaagct ggagcacgat gccgcacgca
     5461 atccttcggc tgccctgggc cgcctgccat cgcgcggcag gccgatgctg cgtggcctca
     5521 tcggcttgct gttggcagct tgcatcttta tcactgcttt cgtttcgcag tcgtcacatg
     5581 gagatgcggc caagctgatt ttcgcccggt gggcgccgca gctcattttg acttcatcgc
     5641 tgccgatgga aaacccgggg cttcccgcgc ggccgagccc atctgccgtt caggtggccg
     5701 ctgcggaacc agtagctgcg caaccaacac ctttggctcc aaccgcaccg cgagacgtcg
     5761 cgccgacagc cgccccggtg tctcccgagg tggcgcagtt gctccagacg atggcgcgcg
     5821 atctcgcaaa cgtagagcaa aggatcgagc agctcaaggc tagccaggaa caaatggcca
     5881 ccgacaatgc cagggccgtt gagcagctca aggcgagcca ggaacaaatg agacgcctta
     5941 tcgccaaggc ttccgagcaa aacctgcggc ccaagacatc agcgcctccg ccccagctga
     6001 ctgcctcccc gacgcgtaag cccgtaccga agcgtccgtc gccacaagcc agagcgcagc
     6061 cgcaggcccc agtgcagttg cagcccgacg aatagcaatt gtcatcggcg ggatactgga
     6121 tctattgcgt gtatgcctaa cccgcagatt gtggctccgg gaacttccat ggcccttcag
     6181 agcttcaggg cgcgcccgat agagccgcac agggtagttc cagccgttca tgattggtag
     6241 gcagttggaa tcatgctgtc gcaaccgccg aaggccggcc cagatggttc gaccaaacga
     6301 atctctgtgc aattgtcggc tgaggttcgt aatttgcttc actacgggat caagtgagag
     6361 cagcgacttc agaatctggc acaaaccgac ggttggaacg tgggcagagc atgtgcgccc
     6421 tgcccctgta gatcagacat caacttgttc tgccatggcc aaagcatcgt cgacttcgat
     6481 gccgagatat ctgaccgtgc cttcgatttt ggtgtggccg agcaacggct gcccggctcg
     6541 caggttagcg ttgcgccggc aaatccgggt cgcattcgtc cgcctgagcg aatgggcgcc
     6601 gaagaccaac ttacatctct actgggaagg ctggccctga ggtgtgctac tcggtgattt
     6661 tggcggggag ctattttgcg gcgagctgtt ttgttcgctg gcctgcgtcc aactcggcat
     6721 ccgcaatgac gaattatcga ggtgattgac aagccacacg cctcccaggg cgataactcc
     6781 cgccaagaat acgtggggta gcgtttgaac aagcacgcca agcaggcctg cgaatgcagc
     6841 caggatcgcc gcacctgctt ccaaaagcgc ccaacctgct ctgtaaagcg cccacagcac
     6901 tccagccagg atcagcagca gcagaacctg cttcaacagc ggccactcct gccatggcac
     6961 ggccatgagt ttgctgatct gatcgaccga ccaggtccag atcagttcga cgaaacgaaa
     7021 gatcgcagaa atcccttgct gcaggaattt caaaagctga tcgaaaacct ctttcatgat
     7081 ccgtggctcc ggcgtttgaa ccgccttttc gagaatccaa gtgtaacgtc gaaacgagcg
     7141 ttgggttccc gccgggcgaa tacctctacg gattttttac cacctcccag gcgatcaaca
     7201 gcaaggtcca cctgcgctat cggtgggcaa cgttgattca tttatgattt ctcgccgcgc
     7261 cccggttgca gaaatgctgc ttctcccgac ccgtatcccg agccttgcaa aaaaccacga
     7321 atgacctcga tcgtgtggaa ctccggtacg gggaagcgcg ttgcttggcg gtcgtgcgtg
     7381 agcatcgcac tgcgtttaag tccgttggcg gcgtgcaagt cgccgctgtt acaggtcgcg
     7441 gttgcggcca tccgggggtg gcgatccttg agttaccagg cagaaatcag ccgaacgtcg
     7501 ccaaccgcca tcgtgatggt gatggaccag tccgcgtcga tgaatcatcg tttgaaaagc
     7561 ggacagagca aggccgcctt tctggcggat gtgctcaata aaacgctcta cacgctcatt
     7621 actacatgct ccaaggctga cggcgtccgc gactattttc acgtgggggt ggtcgcctat
     7681 ggcggggagg acgctcgcaa cggctttcaa ggagcgttgg acggcgaggc actgcaagcc
     7741 gtctcgcgcc tcgccgaagc cccgctgcgg gtcgagacgc gcgccaagaa gatcgccgga
     7801 cctaacggcg aagtggcgga gcagtcggtg cgatttccca tctggttcga gccgcgcagc
     7861 cagggcaaga cggcgatgtg cgccggactc aatgcggcga tcggcatcct ggagcaatgg
     7921 cgcgatgcgc acccggccgc ctttccgccc acggtcttgc acgtgacgga cggccatcct
     7981 tccgacggca atccggagcc gattgccgat cgactcaaaa gtcttgcgac cgacgacggc
     8041 gcctgcctct tgttcaatct gcatgtcgac gcgggcgacg gcgaaccgct gatcttcccg
     8101 aatgacgagc gtgtcatcca ggatcgcttc ggcaagtcgt tgtttcgcat gtcgagtctg
     8161 ctcccgccgc acgctttgga ggccgcggcg gccaagggtt atgacgttcg ccccggcgcc
     8221 cgcggattcg tgttcaatgc cggaatcgaa gccatcgcag acttttttga catcggaacg
     8281 cgtccggcca tcgccgctga ccgctgaccg acccaaatcg agggggatgc cgtgccttac
     8341 caagccgaaa tcagcaggga caatcctacc tgctttctct tcgtcatcga tcaatccgga
     8401 tccatggatg aaataaccga gaccggccgc tcgaaggcgg acttcgtggc cgacgtcctg
     8461 aacaagacgc tctacacgct gatcaccacg tgctcgaagg ccgagggcgt gcgcagctat
     8521 ttcgacgtgg gcgtgattgc ctatggcgga aaggaggtcg cgagcgggtt cggcggcgcg
     8581 ctatcgggcg gcatagtgca cccgatcaac gccatcagcg aacacccgct tcgcgtcgag
     8641 gaacggcaaa agaaggtgga cgacggcgcc ggcggcatcc tcgaactgaa gaccaagttt
     8701 ccgatctggt tcgacccgaa aagcgtgggc ggcactccaa tgcgggcggc cctcgacaag
     8761 accagggaga cggtggcgga gtggtgcgaa gcgcacccgg cttcctaccc gccgaccgtt
     8821 cttcacgtaa ccgacggtca atcgaccgac ggcacgcccg aggaaatggc agacgcgctg
     8881 cggcggattg cgaccaaaga cggccaggcg ctgctgttca acctgcacgt aaccagccag
     8941 ggcggcccgg aaatcctgtt tccgacctcc gaaaccggtc tcaacgacga gtactcgcga
     9001 atgctgctcc ggatgtccag tccgttgccg ccacatcttg ccgagttcgc cgacgacaag
     9061 ggctatgcga tcgcagacgg atcgcgtggc ttcatcttca acggtgatcc ccagtgcatc
     9121 gtggacttct ttgagatcgg cacccggccc aagctagtag ctgatcggtg agcactccgg
     9181 agttgcggca gaaatcgcgc ttgccgttgg cgctcagcat cccgaaaact gaagatagcc
     9241 gcaacgagga cggcttctac cgatcggcca aaggcgtcta tgcgctgagc gacggcgcat
     9301 cggtgtcctt cgactccgcg tcatgggcgc gtattctggt gcgccattac acgcgcaacc
     9361 cgcaattcac gcgcgagtgg ctagcgggcg cgatcgccga gtttcgcaag ttgtacgatc
     9421 gcgaaaccct gccatggatg cagcaagcat cctacgacag gggtagtttc gccacactac
     9481 tgggcgttcg cattgtcgac gaaggaagac tgatccagat cttcgcgatc ggcgacaccc
     9541 tggccgtgct atgcgacggg gatcgcatca aggcaacttt tcctttctcc gccgcatccg
     9601 ccttcacccg gtcgccgaaa ctgctatgta ccaatccggt ggggaatgcc tttctggatg
     9661 aggtggattt cgggtacgac ctcgtcgccg actggacctt caccgggctg aagcaaccgg
     9721 cgctgctgtg tatgaccgac gccctcggtc attggctgct ttcgcagcgc gatcgcaatt
     9781 cgtctccgat cagcgcgctg cgcaagctgc aaacgccgcg ggcgttcgcc cgtttcgtgc
     9841 acgaggagcg cgcggcgggc cgcatgaagc gcgatgacac cacgctgatc gcgctgtggt
     9901 aggctagtca tgtcataccc tcagatcacc gattaccagg aagccgtcca acatccggcg
     9961 caggcattca tcgacccgga cttgaagcag ggcgccgtcg cggaaaacaa tctcggcctg
    10021 ccgctggtga tgtcgggtgg gtttgctctg acctatgcgg tcaccacccc gcgcagaaaa
    10081 tgcgcggtac gttgctttca ccgcgagatt ccggcgatcc agcagaagta cgacgcgacc
    10141 tcgaggaagt tgcgctcgct tgcgatcgga tgcttcgtcg atttcgattt ccagcagtcg
    10201 gggatcagcg tccgccagcg ggtgtttccg atcgtgcgga tggattgggt cgagggcgat
    10261 accctgggta tctggttgga caagaatttc aacaatccgc gcgcgctcga gaaggctcgt
    10321 gccgattttg ccgccattgc gcgttttctc gagcgggagg gaatagcgca cggtgacatt
    10381 cagaacggca atgtgatggt ggcaaatggc gagatcaagc tgatcgatta cgatggcatg
    10441 ttcgtccccg gtatgcatcc cggcaacggc agcgagaccg ggcacaggca cttccagcat
    10501 ccaggccggc gtgtctccaa tttcggtccc gggatggacc ggttcagctt cattgctctc
    10561 gatctgagct tgagggccct cgttgaggac agatcgctgt actcgaagtt ccgtgagggc
    10621 ggcgagacca tcatcttcaa ggccaacgac ttcgctgacc cacagaacag cgaaatcttc
    10681 cgacgtctgc tggcgatgcc gaagctgaag gcgcaggcgg gcagctttgc ggcaatctgc
    10741 gaagcatccc tcgcggcggt cccaaccctg gacgaatttc tctccggccg gaatattccg
    10801 gcggcgaaga caccgatcaa cccaacgacc acgcccgcga caaagccggc ggcggcctac
    10861 atccccgcct atccggtcgt cgatgcgctc aatttttcgg ctgcgctgca acgggtcggc
    10921 gaccgggtcg agttgatcgg gcggatcgtc gaaatcaagc cgggatcccg gaaacagggc
    10981 aaaggtccga acaagcgcta tgtctttatc aattttggct cgtggagcgg caatatcgtc
    11041 aagatctcga tctggtccga tgggcttgcc aggttgaagg acaagccgtc ggccgcatgg
    11101 gtcgggcgtt gggtcagcgt caccggcctc atggacgtgc atgaaaccaa acgctacagg
    11161 cacttgagca tcacggtgca ggaagatggc cagatccagt ggctcgatga atcgcaagcc
    11221 ggcttccgac tggcaagcat cgtgatggca ccgccgcccc gcgaccgcgt ttcgacgggc
    11281 gcggcttcct ccggaagtcc ggtggccgcg ccgccggccg ccgctgcgaa gccgaagacc
    11341 ctccggcccc gcagtcaggg taccgcgcgc ggccacaagc gcgctggccg gcaacagcca
    11401 attccggctg cgaccatcgc ggtccccacc caacaaggga cccaatcgct gggacgcttc
    11461 ctggccgcga ttcctctgtg gatgttgggg atcgggacac tcatcgttct gatcatgttg
    11521 cttcgaatgt acgcgtagac gccgctattg aaccagggcc gccacagagc aaaaggccgc
    11581 ttccgccacg aagccaacat ccgacccgcg tcagtgaatg tccgctatcg aggggagcca
    11641 aagctggctg gaggcgacaa gaacctggag acgacaagac ttcagctttc gaccccattc
    11701 cgacatcaag gtgcgcgcga ggtcgccagg ttttgtcaat gatcgcgata cgcaaatcga
    11761 accctgcgca gatcggtgca acgtccggtc attgagcact cgaagcccgc ttgaagtcac
    11821 gaatgccggt gaccgatacg cttgcgcggg acatctttct tcttgccggt ttccgttggg
    11881 atcatgacca cgcgcccgta tttgacgcca cgctcggcaa tgacgtggtc ggcgaaatgc
    11941 tgaacctccg caccggggcc gcgcaacgcg gtcacctcca tgcagttatc atcgtcgaga
    12001 tgaacatgaa gcgtggccag cgacagatcg tgatggccat gataattttc gaccagccgc
    12061 cgcgacaggt cgcgcgcggc atgatcgtag acgtagacta gcgctgcgac gcactcgccc
    12121 gattttcctt tcgcgtgcgc cgcctgctgc atcccggcgc gggcgaaatc gcggatggcc
    12181 tcggaacggt tttgataacc gtgctcggcg atcatggtgt cgagctcctc catcagatcg
    12241 tcgtcaaggg taatggtgat gcgttgcatg gcgtcttcct gggcacgaga agtgtgatga
    12301 tttattgata ttgtcatact cggaatgcta gtgttatctg gtgttgtcta aaccatatcc
    12361 cgcccggtgg agctgcggga cttattcatg aagcttgttg ccgctcgggg gtggtgatgc
    12421 atcggtcctc acgcggattg gctgcgatct ggttggttgg ttgggttgcg gtttcaccca
    12481 ttgtatctgc tcaagacgcc aagcccatcc cgcttccggc agtgcagatt gatgcgcctc
    12541 agcagcgtca ggatgcccgt ttcaagcgaa agcacagtgc ggacagaggc ggacggtcga
    12601 accgcactgc gatccaaaat ccggtttccg tcgccaacgc tgcgcccgct cagagtgcgc
    12661 ttggcgcgtc ggcggcggca gcacctgtcg cgtcttcgag cgagaaaaat gtcagcggcg
    12721 aggaagtcaa cgcgcggccg ttttcccggc cggcggaagc acttgaagtc gtacccggcc
    12781 tgatcatcac ccagcacagc ggcgacggca aggccaacca gtatttcctg cgcggcttca
    12841 atctcgacca cggcaccgat ctggccatct cggtcgacga catgccggtc aacatgcgta
    12901 cccacgcgca tggtcaggga tacgccgact tgaatttcct gattcccgaa ctgattggtt
    12961 cggtcaacat ccgcaagggg ccctatttcg ccgacgaagg cgacttctcc tcggtgggat
    13021 cggtccatgt cggattgctt gataacgtca acaagacgat ggcgtcgatc accgccggaa
    13081 gcttcggcta tcgaagaatt ctaggcatca catccacgaa actcggtgac ggcacgctgc
    13141 tgatcgcggg cgaagccaac acctacaacg gcccgtggga taatcccgac agcttgcgca
    13201 aactcaacgg cgtgatgcgc tacggccagg gcacggcaga ggacggcttc tcgctgaccg
    13261 ggatggccta tgcgaataaa tggaattcca ccgatcagat tccatcacgc gccatcgctt
    13321 ccggcgagat cggcctctat ggcgcgttcg atcccagcga tggcggaaat tccaatcgct
    13381 tctcgctgtc ggggcgctgg gcgcagaccg acgagggcgg tacctccaag gccaatttct
    13441 acgtcatcaa gagttcgctc aacctctgga acaatttcac ctattttctc agtgatcccg
    13501 tcaacagcga tcaatttcac cagcacgacg accgcgttct cggcggtatc aacgggtcgc
    13561 atacgttcaa aagtcagttt gcaggcttgc cgatggagac cgaaattggc gtgcaaaccc
    13621 gatatgacga tatccggctc gacctgaaca acagtgtgca gcggcagttc ctgtcgccca
    13681 ttcgcagcga tgccgtacaa gagggaagcg tcggagtgtt cgcgcagaac accctgcact
    13741 ggaccgactg gctgcgaacg aatgtcggtt ggcgcggcga tttctaccaa acctcggtca
    13801 attccttttt caccccagcc aactccggca gcgccaatgc tttcattggc agtccgaagt
    13861 tcggggcggt gtttgggcca tttgccaaga ccgaattctt catcaacgcc ggcgagggat
    13921 ttcatagcaa cgacgcccgc ggcgtgacga tcacggaatc acccggcgat ggctccgcgc
    13981 tgcagccatc gccgtttctg gtcaagacca ggggagccga agtcggagtg cggagcaagc
    14041 tcgtccccgg ccttaccagt tcgatgtcgg tgtttttgct cgactcagcg tccgaaattc
    14101 tattctcggg cgatgccggc gatacccgac cgagccggcc cagccgccgc tacggccttg
    14161 agtggaccaa cgactatcgt ccgctgtcat ggctcagcct cgaaggcgac attgccgtga
    14221 cacatgcgcg gttccgtggc gacgatccgc ctcaggcggc gctgtatgcg tcgctcgccg
    14281 ggtttcctgc cgcgcagatc ggcaatgcgc caggcaacta cattcccggt gcaccgagca
    14341 tgattgcatc tgccgggatc agactcggcg aaaggaccgg ctggttcgca ggcttgcgct
    14401 atcgttattt cgggccgcgc ccgctcaccg aagacaatgc ctttgtctca ccggcaaccg
    14461 ggcttctcaa tgggcaactc ggctatcgct ttgataatgg ctggcgggtc caactcgatg
    14521 catttaatct gcttgacagc cgctccgacc agatcaccta tgcctatggt tcgctgctca
    14581 aatcggattc gctgttcgcg atgtgcttcc ccgcggcggg ggcgccgacc gcacctgccg
    14641 ccgtgtgcca gaccggcgtc atggacggcg tgctgcatcc ggtcgagccg ctagccgtcc
    14701 gcgtgacggt agcgggacaa ttctgaattg cgtgtcgcga tccattttcg ccggcggccc
    14761 ttgccccgac gataaggtga tcgaatgcca ttttgtccgc ccctggggcc gttggtggtc
    14821 tcccgggtcg agttcccgaa agggtgtcat cgttagcggc cccatactct ccaccgtatc
    14881 gccttgcttt ggtcaaatct gagtggagtg tcgggcagaa cgtcgtcgat ccagcgtcaa
    14941 tccgccaccc gccttatgcg ggtgacatgg gactgagtac ggagcgccaa tgcaatctac
    15001 gccgaacccg aatcaaaccg aaccggacga tctccttgtg atcgcgcccg atatcgttct
    15061 ggtcgcgcgc gcggacaagg cgcacgacgc cgtcagccgt cattctgact cacagaccca
    15121 catggcaccc gacttctccg ccggcccatc ggttctgccg gtcgatacga cgtttcgcgc
    15181 cgcggccgtc gacgatgtcc aggtccgggg cgacgggcgg tcgattggcg ggcgcgtgat
    15241 acgcgcctgc attggcttcc tgttggccgt atgtctcgct gtcgcggcct ccctgtggcc
    15301 gtcctacggc gatgcggcca aacagattgt cgcgcggtgg gtcccgcagt tcgccctgac
    15361 ttcatcgtcg ccccaggaca accccgggct ccccgagcaa ccgggcccag ctgctgtaca
    15421 ggcgtccgcc gcgaaggcag aacctccggc tcagaccgcc ccggaaagcg tcgcgtcgac
    15481 cagcgccacg ctatctcctg aatccacgca gttgctccag tcgatggcgg gcgatctcgc
    15541 acacgcacag caggagatcg agcagctcaa ggccagcatc gcggagctca aggccagcca
    15601 gcaacaaatg tcccgcgaca cggccaaggt ttccgaagcc agggtttccg gggccaggat
    15661 tgccgaagcc aaggtttccg agcagaacct gcgatcccgg ctatcagcgg ctgtgcggcg
    15721 gtcagcgtcg gcgcgcaagc cgatgccgcc attgccgccc ccgcaagccg ctgcggctcc
    15781 tccgttaccg caagccacga ctcagccgca ggccgaactg gtgccgcggc cgccgatgcc
    15841 cctgcgctag cgccgttcac cgcaacttgg aaaaattcgt ccacaggcca taggcgatgg
    15901 gatgcttcgg ataatcggga cgatttgtct cgccgtcagc acaacatctt catgatttcc
    15961 gctaggatac agcgaggccg caatctgacc ggggcgaatc gaactgggtt cctgcgctgg
    16021 aaccaaaccc ggccaccgcg acttatcgcg catactcaaa aaccgggaga ctgccatgaa
    16081 aatcgcaaag atcatgttgg ctggcactgc ggcgctcacc atcatcggct cagcggcctt
    16141 ggcccaacaa gcgctcacgg gaacggtgac caaagttgac cggatcaatc gcagcgtcgc
    16201 aatcaaacag acgcaaaccg gtaccgtcgg agcgaacacc ggcggtgctg ccgaagagtt
    16261 caaggcccag gacggcttgt cgctggacac gttgcatgcc ggcgatacgg tcagcttttc
    16321 cgcgacggaa acgggtgggg tcaagacgat cacgaaactt cagaaacagt aattattcag
    16381 cacgatccga accggacatc agcaagccca atgtgaattg ggatcgtcac ttgcatttca
    16441 gtcggagttg gcacctcatg catgtcgctt gacgaccccg gtcggcgtgg cgaacccttc
    16501 gatggttcga attcgcttct cgagccacca gccgaattcg ggagtccaat cctcgaactt
    16561 ccggttgata ccgagatcat gagcccagtg ctgggcgata ttgggattga actgggattg
    16621 ccggccgacc gcgatgaggt cggcctgccc gttctccagg attgattccg cctgctgggc
    16681 ttcgagaatg atgccaaccg ccatcgacgc gatgcccacg tccttgcgga cccgttcggc
    16741 gtagggcacc tggaagccga ggccgcgcgg gacttgcgct gccgtcgccg aacctgaaat
    16801 gccgccggag gagcaatcga tcaggtcgac accgcgggcc ttcaattccc gggcgaagat
    16861 aaccgtgtca tccagattcc agccgttctg agcgccatcg acggcggaga cgcggacaaa
    16921 caacggcatc gttgccggca tctcgcgccg cacggcttcg acaatctcga gcggcaggcg
    16981 catgcgcccg ttacggtcgc cgccatattc atcatggcgg gtgttggaaa cgggggaaag
    17041 aaacgaggcg agcaaatagc cgtgggcggt gtggatctcg atcacatcga accccgcatc
    17101 gacggcacgc cgggccgctt ctgcccaggt gcgcaccatc gccctgcact cttcggtcgt
    17161 gagctggtgc ggggtaagcc atccgttcgc gaccggctcg ctggtggggc ccaccggctg
    17221 ccacaccttg gcgcccgcct tgatttcctg cggggtgagc gggcccatgc catccatcgc
    17281 ccgctgcgac gacgacttac gcccgccatg ggcgagctga atgccgatcg cggccttctc
    17341 gcccttcatg aaggcgatga ggggcctgaa gctttgcacc tgcgcatcat tccagatgcc
    17401 gaggtcatgc tcgttgatca gcccaacctc ttccacagca gtcgcctcga caaagacaag
    17461 cccaaaacct cccagcgcat agcggccgag atgcacgtga tgaaagctgc cgcaggtgtt
    17521 gccggggccg gcgcggtaat ggaccatggg cggcacgacc aggcggttct tcagcgtcag
    17581 gccacggatc tcgaacggct ggaacaacat gggccgcgac aattcaacgc tatcggtgac
    17641 cggggctgac atggggttgc ctgttgagtg agtggccttg cgtggggcaa ccgaataatt
    17701 ctctatttcg agtgataggt ccagcaggct ccttgcgaga gatctcgtgg ccacacatgc
    17761 gtgccgagat ttctcgtcat ggcaccttgc agactttgcg ctcgcgccag gcgatggccg
    17821 gtcgtaagga taaatccgac aggcgcctgg ccgggatcag gggccaaaag tgttattcgc
    17881 gctcgccgtc cggccgcggt gccaggcttt agtcagtgaa aaggatattc catgcgtatt
    17941 gcggttgtcg gcgccggggg tgttggaggc ggattcggcg cggcgctggc caaggccggc
    18001 gccgacgtca tcttcatcgc gcgcggcgcc catcttgccg ccatgaagcg cgagggactg
    18061 aaagttcagg gcggccgcgg cgagacccat ctggtgccga cccaggcaac cgacgatccc
    18121 gccagcatcg gcaaggtcga catcgtgctg ttttgcgtca agctgtggga cgtcgaaaac
    18181 gccggccaaa aaatcaggcc gctgatcggc cccgacacgg cggtcatccc actgcagaac
    18241 ggcatcgacg ccgccgagcg gctgatcccg atccttgggc ccgacgccgt gatgggtggc
    18301 gtggcgcaga tcagcgcgtc gatcgtcgag cccggcctga tccagcaggt cggcagcttc
    18361 atgcggatga tcttcggcga gctcgacggc aagcgccgca agcgaggcga ggattttctc
    18421 gcgctttgcc tgcaagccgg ttttgatgcg accttgagcg agcagatcct gaccgagctg
    18481 tggatgaagt tcatcctgct cgccggcaac gccagcatca tggcgctggc gcgccagccg
    18541 atcggcaggc tgcgggacga tcccgacctg cgtccgattt tcatcgccgc ctaccgggag
    18601 acgatcgaca tcggccgcgc caggggcgtg gcgctgccgg ccgacgcgct tgagaagatt
    18661 ctcgacttta ccggccacgc gccgccggcc atgaagccgt cgatggcgct cgatctcgag
    18721 cgcggcaacc ggctcgaact gccctggctc ggcggcaagg tggtggagct cggccttgag
    18781 cttggcgtcc cggcgccgac gcacagcatg atgtatgcga tgctgaaacc ctacatcatg
    18841 ggcacgcctg cctgagcagg cttgcggttt acgccggcct tcatcgcacg gagctgccgc
    18901 cgatttggct tccaggccga ccaaggtgac gatcatctgc aggttattct tggacgaatg
    18961 acccgcccca aacgccctgg gaacccaacc aactccggcg acgataacgt cgatgaagcg
    19021 tggtcaattt tgctcaggcc accaataacg gtgacatcct ttcttttaac ttgccaacag
    19081 ggtcttctcg gcattctgtg ccgcatggaa agacaagacc cgcgctagat tggacgcctg
    19141 tgcttgctac tccgaaactt ctctgggttg gaaacaccac ccgttccgtg cagggatttt
    19201 tttgactata gggtcagata ggccacggcg acggcagaac gacaagcagg atcatcgcag
    19261 cgctcgaatg cctatgacga gatcatcgaa tggctgaagc gccgtgagca acgcaggcgc
    19321 gacaagacca caagcgcagc gaacagtaac cctgcccggg agtcgaccag ccagtagttg
    19381 gcagttggtg gcagttggtg tataaacatc tccatgagtt gggatgcgga agacgtagca
    19441 ctgctcaaga aactctgggc cgtagggcgg agcgccgcac agatagcgcg ccgcctcggg
    19501 tatagccgta acgcggtctg cgccaagcta acccgtttgg gcctgaagcg cggtcacaag
    19561 ccgccaacag caaaacccag gataaggccg gtgccgaagc gaaggccggc gttgttggct
    19621 gcctgtgcca gcccggtggc aaagaaggtg tcgcggaaga cagcaatgcc gaggcccaag
    19681 gaattcacaa agcgccagct ttacgccatg ctcgctgaag cggtcaggaa cacgggctag
    19741 ggctcacaag cacctggtcg cggcgaaatc cattcggtgc cgacgacggg actatcggcc
    19801 ggccgcaaca ccgtcagtca gtaaccgcca agacaaaaaa acgggaccca aacgcatctc
    19861 tggcaggagc agaacgatgg ctctcctaaa acgagatttg tatcgtcagg tggaaggccc
    19921 tgaggtcacc cacgcggatc gctgcacgct ggtcttcgat accgacagca aaaatttata
    19981 tgtcgagcgt gaggtagcgc atttggacgc gaaggtcggg ggcaccgtcg actaccagac
    20041 agcgacgatg gatatcgcgg attatctcaa gcaaggcggc caaaccgccg ggcaccgcga
    20101 gctatggcga ttgctgagat cgatcttcaa actggacagc gatgctgacc aagcgatcta
    20161 gccatatcgt ttccccagcc ttttgggcct cttgaggaac attcgcttgg cctttgcctc
    20221 cgcacgcttt ttgatctcgg ccttgatctg ttcaatcaag gttctcgctt ccggcgtgtg
    20281 aactttgagg gtcgaagtca tttgcgtacc ccctctgaca aactcacgtt gagacactaa
    20341 tcaaatttca gaacagggca atggcaaaag cgcttgaaga ccaaggcccc gcggaaatca
    20401 gcgggatcgg atttaccact gcggcagtcc aagacacgga acccaagggg agcggtcggc
    20461 tgggtcccgc gcatgacact gcagcggcgt ctcggaccgc cgcatccggc gcaagatcca
    20521 gcttgtcgaa gttcgcgttt gaagtaccat ccaagctcga tcgccctgca gcgcgggtgg
    20581 agagttgaat gtcgggtcgt gctctcacag gcgtggcagt tctggccgcg ctggccgttt
    20641 ccaacccaat caccacggcg caaactgtac cgaaacttga ctacgaattt ttcaaaagcc
    20701 gcgtcgagcc ggtctttctg accaagcggc ctgaccatgc ccgctgctac gtctgccatg
    20761 tcgagagcaa caacgcattc cacctggaga aacttgcacc tggggcacgc gcctggaccg
    20821 aggaacaatc gcgccgtaac tttgagatgg tctcggtact cgtcaacccg ggggatcccg
    20881 atacaagccg gctgctgctt catccccttg gccccgaagg cggcggagat gtgtttcact
    20941 ctggcggacg gcagttcgcc tcgaagcgcg atccagcctg gcgcatgctc gccgcgtggg
    21001 ttaatggagc gacgctcgct tctcagaaga aatagcggcg gagcctggat ggtccgggat
    21061 aacaagcgtg ttgatgcgct tgggcacctc gccgaccggg accacggcaa tcagcttcat
    21121 tgatttggta tcgattgccg agactgagcg cagacctgcg ttggaaatgt aggccgtctt
    21181 gctgtcgggc gtgaaggtga cccaatttgc gaccgccgaa atcgatccgt gccccggcag
    21241 cgtgagggac ggaagcgcga cttcgccggc gagcgctagg tcggcgagcg aataaacata
    21301 caccgcgttg ttggggatgc tggtcaccca caggctcttg ccgtcgggtg caacggctat
    21361 gccgtgtgaa ggcgccgtgg cgcggtcccc gtcggtctca aactcggttt tggccgccgg
    21421 cagctggacc cgtgccactt ccttgcgtgc ggcaaaatcc accaccgcga agccgttaaa
    21481 gtcggacaat tgcacaaata ttcgtttggt cgagccatct ggacccgcct cgatcgccat
    21541 cgggcgaatg cccttgtcga gcctgacctc ccacgcgggc tgctcggtcg cgagatcgat
    21601 gaccgttaca attccggttc ggatcgaacc ggtgacgacg tatttactgt caggtgttac
    21661 atagacgtta tgcagcctgc cgtttaccgg cacgcttttg gtccgcgtca aggttgccgg
    21721 atcgatgata tcgagcgcgc ccggatcgcg ggcaatgccg accacgatgc ggccgtccct
    21781 ggccaccgcg atattgttgg ggtgtgcgct cagcgccacc ttcttgacca gcgcgccgga
    21841 cttccggtcg aaaacatcaa gcgtggaatc gacttcatcg ctgacataga ccttggatcc
    21901 gtcaggagag aatgcaatcc cgtgcgcacc ctcgatgccc ttgatctgct ggaccacctt
    21961 gttggtggcg ggatcaatga cgtgaatgct gtcaccggcg ctgttggtga catagacgcg
    22021 gaccgtgccc gcccaggccg gaaccggtgc aaccatcaac aagatccaga tatggcgtaa
    22081 gaagcgatag acgttcatgg gtcgagctcc cattcctctg acctgcgctt gctcagaatc
    22141 tcagtgctca catcggcggg atcacgccgt ccttgccgaa aagaaccgca ccgctgtgca
    22201 aggagccgtc cgactgacgt tcggtcggca cgaacacgat ggcaccgggc gcaatatcgt
    22261 ccttgcttgc tggaacaaga gcaactaccg gcacattggc ggggatatca attttctttt
    22321 cgccaccctt ataagtcacg gtcactgttt gaccgtcgac ggccttaacg gcgttcgcca
    22381 ccgtcgcatt tgtcatcatg cttgatgagc cgagatccca agggtagtgg ccttcggcgg
    22441 tgccgcgcaa actatcaggg aatacgacga cttccactga tcgcaaggtt gtatcgggtt
    22501 gggttacggt cgcggtgcca atgaacgtgc cttccttgat atccgccatc gacgacttca
    22561 cgacggccag cgcagcaaaa ttgtcggcca ggctgacgga gacgtcttta ccgtccttgg
    22621 cgcgaacgac gagcttggac ccgtcaaggc tcacaacagc gccacgcacg cggacggggg
    22681 cgccatccgc atagactggc gcgcaaccca gcgctatcgg aatgagaagc gcgccaaaag
    22741 cggcgcgcgc cagacgtctg tgaatactca tgaagcacct cctggatggt cgaggaggcg
    22801 gcaacgtagc gcgctggcgg aaaactgtca gcgtcggatt cttgtccggt atcactatcc
    22861 cgcgatgtcg ggatcgtcag gaagcgaagc gccggcgacc cggcgggctt ttcctggctt
    22921 caccagccgt tgccgcgctg caggcgatcg acggcgataa caaggtcggg ccgctcgagc
    22981 acttcgacca gcctgtcaag gatgcgctcc tggttgtgcg ggcgaggctc cagatattct
    23041 tcgaggatgc gctgagcctc gcttacggcc tgaagcgcca attgctgatc ggtcataact
    23101 tctccttgaa cgcagagaaa cagacctaat gatattaagt catattagtt ccatgggtct
    23161 ggccgctcaa tggccgcgaa atggcagcga cattgaccca tcaaaccggc gtggcaaaaa
    23221 agccgatggc gagggcgaac agcatcagtc cgccgaacgc gtagattgcg atatttacgg
    23281 gactggtcat tggagcataa ccctgccgac taatatcggt tccgaagcaa ttacagtctc
    23341 cagcggacga ccgcgccaac cgagttggtc tccatgacag gttttccagc cttgatgata
    23401 cgctccgcat gggcggatcc atcgccgctc gtgtcgcgaa atgacagggc gagacccgct
    23461 acaacaatcg ctgcgcacaa cgcgaccatc acgattttaa ggtgcgtaag gcggtcggcg
    23521 ctataaatgg aatggttcat ttgagcctcc tgcacctttt ccggcgctta cctaggagac
    23581 taggtcgcga ttgtttcaaa atggttgcgt gagaaggaca aaatggtttc atcgcaggga
    23641 atgaaggggg ttgtcgccat ccgacggcct gattggtttg aacctggctg tcaccgggaa
    23701 ggttttttgc aggtcgcctt cgacgcatgg acggctgcca aacgaacgca tggatccgcc
    23761 ccgccatcct atctatctcg cacccaacgc tgccgcgtgc accgcatccc gcccctcgtc
    23821 ggcggcgatc gcgataccca gggctgaccg gcgcatcgcg gcaactccgg gcaaatggca
    23881 tcctgatatc catcctccgg ttgcatccct tgataacgcg gcaaatgcag ccggatgctg
    23941 gcatacggga tgcagatagt cgcatctagt cgcatcgctc agcgaggcgg gcccggcgct
    24001 tgcgacctcg actcccatcg ttatgatcga agagaggtgc accaggcgcc cgccatatca
    24061 gggagagacc cagggcctgc gccgggtgca gcagaccaac ccggaaggca tcgcgacagt
    24121 cgacggcgac cgccgccgct ggcgcgagat cggcgagcgc gtggcaaggc tcgccggcgg
    24181 cctgcgagag ctcggcgtga accgcggcga ccgcgtcgcg gtgctgatgc tgaactccga
    24241 ccgctatctc gaactctatc tcggcatcgc ctgggccggt gccgtgatcg tgcccaccaa
    24301 catccgctgg agccacgccg agatcgagga ctcgctgcac gactgccgcg cctcggttct
    24361 ggtcgtggac aaggcctttg ccaggatggg cgtcgaactg gcaaaggccg ttccgctggc
    24421 cctgatctat gccgatgacg atgccggccc agccgaggcg cagaactacg aaaaactgat
    24481 cgctgcgagc gagccggtgc cggacgcgat gtccacgcgc gaagagcttg ccggcatctt
    24541 ctataccggc ggcaccaccg gccgctccaa gggcgtgatg ctgagccacg ccaatatcgt
    24601 cagcaatgcg ctgcacatgc tgagcgaagg cctgttgccc gacggcacga tctatctcaa
    24661 cgctgcgccg atgtttcacc ttgccaatgg cggcgcgatg tttgcttccc tgatcggcgg
    24721 cggcaccaac gtgatcgtgc ggacgttcaa cgcagaactg gtgatggaca ccatcgagcg
    24781 ggataaggtc acggccaccc tgatcgtgcc gaccatgatc cagatgctgg tcgatcatcc
    24841 cgcgttccag accgccgacc tgtcgtcgct gcagcggatc atgtacggcg cctccccgat
    24901 ctcggaagcg ctgctgaacc gcgcgatgtg ggggttgccc ggcaccgcct tccaccagct
    24961 ctacggcatg accgaattgt cgccgctggc gacgcatctg ccgtgggacc agcacaccgg
    25021 cgaggcggct tcgaagaaaa accggcagcg cgcctgcggc cgcgccgcga tcggctgcga
    25081 ggtcaggatc gtcgatgccg cccgcaaacc ggttgcatcg ggcgtggtcg gcgaagtcgc
    25141 ggtgcgcggc cagaacgtga tgatgggcta ttgggagcgg ccggaagaaa ccgccaaggc
    25201 cgtcatcgac ggctggatgc acaccggcga cggcggctac atggacgagg aaggctatgt
    25261 ctatctggtc gaccgcgtca aagacatgat catttcaggc ggcgagaacg tctattcgat
    25321 ggaggtggaa aacgccatcg cgcagcatcc ggccgtgtcg caatgcgcgg tgatcggcat
    25381 tcccagcgag caatggggcg agaccgtcca cgccttcgtg atcccgaagg ccggcgccca
    25441 cgtcaatgcc gccgaaatca tcgcgttctg caaggagcgg atcgccggct acaaatgtcc
    25501 gcgcagcgtc gacgtcagga ccgaaccgtt tccgctctcg ggcgccggca aggtgctgaa
    25561 gcgcgagttg cgaaggcttt acgggcaaga cagcgcgctg gcgcaggcca aggctggatg
    25621 attcacgccg cgccgacttc gaacgcctcg ccgtcgtagc cggcctcgag cggaatccga
    25681 agctgacgaa cgccgtcgcg cttggtcacc acgggcatca cggtgacgat cggtttgccc
    25741 atgctgtcgt cgagcgcggc cttcacgttg ggctgcttgc cgagcctgat caggttgcgc
    25801 gtggtcggaa acggcagctt gaaccgcccg ctcccgccgc cttcgagcgc ctcgcgcggc
    25861 gacacccaga tcgaatcggt ggattccttg ccgtcgtggg cgccgacctg ttccggcggg
    25921 gctgcggcaa gaaagaacca ggtgtcgaac cgttttggca ggccttccgg cgtgatccag
    25981 tgcgcatagg gcaccagttc gtcgagtgcc agcaccattc cgttatcggt gagaacgttc
    26041 aggaaggtcg tcttgccctc gcacaaggcg gcgcgatggt cagcctcgat cgcgctggcg
    26101 cgcctggcat cgaccagcgc tttcgattcc cgtgggcgcg ccagcaggat gccgctttcc
    26161 tcaaaggttt cgcggatcgc cgcgatccga aaactcaagc tcgccgtgtc gagcccctcg
    26221 ccgccgcaat aaagctccgg gctcgcgatg atgtcctgat cgcccttgtc gacactgccg
    26281 ccgggaaaca ccagcgcgcc cgagttaaag tcgatctcat aatggcgcac catcatgaag
    26341 acctcgatct cggccccggc gccgtcgcgc agcagcaaaa tggtggatgc tggacgcggt
    26401 gcgacggctt cggtcaccaa atcactccgc agcacttgat tgcggctgca aattcgcacg
    26461 cttgtcgacc cgcgcgaccc gcgatagcag ataatccacc tcggcctttg cagttgccgt
    26521 aatgctcgaa cccggcttgc gttgcgcgct ggaggcgatc acgccgcgct tttgcaacac
    26581 gtatttgcgc accgccaggc ccgcgcctag ctgctgctcg tagcggatca gcggcagatg
    26641 ggcatcgaac agatcatgcg cggcgtcgcg cttgccggcc ttcgacaggt ttaccacgtc
    26701 gatcagcaat tccgggaagg cgtagccggt catggc
//
LOCUS       DATNOI010000650         3606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2245360,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000650 DATNOI010000000
VERSION     DATNOI010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2245360"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            890..2098
                     /locus_tag="VN968_26260"
     CDS             890..2098
                     /locus_tag="VN968_26260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXL32770.1"
                     /translation="MTPRADFASQDYFRDPGAGIEKLRTLGPVVEVRFPIIGKLWTTT
                     TQALGDQVLKDTATFTMRKENGDVAGLQWWMPGIVRTLATSMLSMDEPDHKRLRDIVD
                     EAFRRRAVLDMEPHIQAMGDELADQLFAEGSPADLVERYARKLPLSVISELLGLPLAD
                     RAKFAAWAAGFTRFTGALGFLGMIPKMLAMKRYIEQHLETVRRQGGEGLIAEIVRVEK
                     DGGQISRNEIVAMVFLLLFAGHETTTHLISGSVFELLKNPDLRDWLEEDWSRVDLAVE
                     EFLRFITPVQFTKPRYVRKDIELGGVRLAKGDKIMVMLAAANMDPRANPDPDRLDLQR
                     KPNRHIAFGTGIHFCLGHQLARIEGRCALKSLFRRWPALTLAVDQSEITWRKRPGLKA
                     IDHLSVAPGS"
     gene            2408..>3606
                     /locus_tag="VN968_26265"
     CDS             2408..>3606
                     /locus_tag="VN968_26265"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXL32771.1"
                     /translation="MDAKTSIKKRLPSRHVTEGPERAPHRSYLYAMGLTSQQIHQPFV
                     GVASCWNEAAPCNISLMRQAQAVKKGVASAGGTPREFCTITVTDGIAMGHDGMRSSLP
                     SRECIADSVELTMRGHAYDALVGLAGCDKSLPGMMMAMVRLNVPSIFIYGGSILPGNF
                     RGQQVTVQDMFEAVGKHSVGEMSDADLDEIERVACPSAGACGAQFTANTMATVSEAIG
                     LALPYSAGAPAPYEIRDAFCTTAGKKVMELIAQNIRPRDIVTRRALENAAAVVAASGG
                     STNAALHLPAIAHECGIKFDLFDVAEIFKKTPYVADLKPGGRYVAKDMFEVGGIPLLM
                     KTLLDNGHLHGDCLTVTGRTIAENLKSVKWNPHQDVVRSADKPITVTGGVVGLKGNLA
                     PEGAIVKV"
BASE COUNT          718 a         1085 c         1137 g          666 t
ORIGIN      
        1 atcaccgggg agagcacgaa ggaaaccgtt aaaaccattg cgtgcgggaa cgtcgggtga
       61 ttccggggtg ctcgttgtta ctcgtgtgcg ttctaccaat accaagtgca cacgagaccg
      121 cgggtgcaac gggcatccgg cgttccccac gccctctttg ggcggaagat tcatcaacgc
      181 ctcgggcgca tagcgtcgcg agggcgcgaa cgtgtgtttg atggatacga gcacgccaca
      241 ctcccagtcg tcatcgcccg cgaaggagcc tgtgaaggat actgacctca tcctgaggag
      301 cttgcgaagc aagcgtctcg aaggatggac gcaacgcagg gactcgcgac catccttcga
      361 gacggcgctt gcgcgcctcc tcaggatgag gtcggagata tattcagagc ctcgaaagcg
      421 gcgatccagt attccgaggc gtcaatgatg aagtcgagaa gccgcagcgt actggatact
      481 ccgctggagc ctgtcatcgg gctcgccgaa ggcgagaccc ggtggcggag tatgacgatc
      541 tttgcgagga gcggcttgtc ccgccgcagc tcaacgagcg actgcgtccg ccgtagctcg
      601 cagagcgaag gcggaaggcg gaagcgacga agcaatccac cctttcttgt tgctctatcg
      661 atcgcttcgc ggagcgggat gaaatcgaga ggcgccgtta tggaaccctt atgcgctctc
      721 aacccgcccg atcttgaatt actgcaacga gcttcgcatc tttcgcgtga gcccggcggg
      781 accaaaaact gaactgaacg caagcgatgg catttgttgt tgagggtggc cgcgccggca
      841 cacccgcgca tcaccttcgg aacgcctggc ttcggtaagg gaatctctca tgacgccccg
      901 tgcagacttc gccagtcagg actacttccg cgaccccggt gcggggatcg aaaagctgcg
      961 taccctgggt ccggtcgtgg aagtgcgttt cccgatcatc ggcaagctct ggacgacgac
     1021 gacccaggcg cttggcgatc aggtgctgaa ggacaccgcg acgttcacca tgcgcaagga
     1081 gaacggcgac gtggccggat tgcaatggtg gatgccgggc atcgtgcgca cgcttgccac
     1141 cagcatgctg tcgatggacg agcccgatca caagcggctg cgtgacatcg tggacgaggc
     1201 gtttcgccgt cgcgcagtgc tcgacatgga gccgcatatt caggccatgg gtgacgaact
     1261 cgccgatcag ctgtttgcag aaggcagccc cgccgatctc gtcgagcgct atgcgcgcaa
     1321 gctgccgctg tcggtgatct ctgagctgct cgggctgccg ctggccgacc gcgcaaaatt
     1381 cgcagcctgg gctgccggct tcacgcgctt taccggcgcg ctcgggtttc tcggcatgat
     1441 cccgaagatg ctggcgatga agcgttacat cgaacagcat ctcgagaccg ttcgccggca
     1501 gggtggtgaa gggttgatcg ccgagatcgt gcgtgtcgag aaggacggcg ggcaaatcag
     1561 ccgcaacgaa atcgtcgcca tggtgttcct gttgctgttt gccgggcacg agaccacgac
     1621 gcacctgatc agcggatcgg tattcgagct tttgaagaac cccgatcttc gcgactggct
     1681 cgaggaagac tggagccgcg tcgatctggc ggtggaggag tttctgcggt tcatcacgcc
     1741 ggtgcaattc accaaacccc gctacgtgcg caaggatatt gaactcggcg gggtcaggct
     1801 tgcgaagggc gacaagatca tggtcatgct ggcagcggca aacatggacc ctcgggcaaa
     1861 tcccgatccg gaccggcttg atttacaacg caagccgaac cggcatatcg ccttcggcac
     1921 cggaatccat ttctgccttg gccatcaact cgcgcgtatc gaaggcagat gcgcgctgaa
     1981 atccctgttc cggcgctggc ccgcgctgac gctcgcggtc gatcaatcgg aaatcacatg
     2041 gcgcaaacgg cccggcctca aggcgatcga ccacctgtcg gtcgcgccgg gaagctgaga
     2101 tcgcggatgg gcggccgatc gatcagggcc gccagcgtcc agttcagccg ttccggctcg
     2161 caacctgcga ggcgcgacag attttagcca caaccatggt ggcttcagtg ccacgattgt
     2221 tgccggtccg caacacttag cgaacatttg gggccaagac cggcctggct attgcctgcg
     2281 ccgcttttta gccacacccc tccgtgacat catatgtagg aaaaaattgc cgtggcgctc
     2341 gcgaaggcga tgggaaattc agcgtttccc cggaaccgaa gtgtgtagct ggggaaacag
     2401 gttagcaatg gacgctaaga ccagcatcaa gaagcggttg ccgagccggc acgtgacgga
     2461 gggcccggag cgggcgcccc atcgctcgta tctttatgcc atggggctga ccagccagca
     2521 gatccaccag ccgttcgtcg gcgtggcgtc gtgctggaac gaggctgcgc cctgcaacat
     2581 ctcgctgatg cgtcaagccc aggcggtcaa gaagggcgtc gcgtccgccg gcggtacccc
     2641 gcgcgaattc tgcaccatca ccgtcaccga tggcattgcg atgggccatg acggcatgcg
     2701 ctcgtcgttg ccgtccaggg aatgcatcgc cgattccgtc gaactgacca tgcggggcca
     2761 cgcctacgac gcgctggtcg gtcttgccgg ctgcgacaag tcgctgcccg gaatgatgat
     2821 ggcgatggtc cggctcaatg tgccgtcgat cttcatctat ggcggctcga ttcttcccgg
     2881 caatttccgt ggccagcagg tcacagtgca ggacatgttc gaggcggtcg gcaagcattc
     2941 ggtcggcgag atgtccgacg ccgatctcga cgaaatcgag cgggtcgctt gcccgtccgc
     3001 cggcgcgtgc ggcgcacaat tcaccgccaa caccatggcg acggtctccg aggccattgg
     3061 gctcgcgctg ccctattcgg ccggcgcgcc cgccccttac gaaatccgcg acgcattctg
     3121 cacgaccgcg ggcaagaagg tcatggaact gatcgctcag aacatccgcc cgcgcgacat
     3181 cgttacgcgc cgggcgctgg agaacgcggc ggccgtggtc gccgcatccg gcgggtcgac
     3241 caatgccgca ctgcacttac cggcgatcgc gcacgaatgc ggaatcaagt tcgacctatt
     3301 tgatgtcgct gaaatcttca aaaagacacc gtatgtcgcg gatttgaaac cgggcggtcg
     3361 ctatgttgcc aaagacatgt tcgaagttgg cggcataccg cttttgatga agacgttgct
     3421 cgataacggc catctacacg gcgactgcct tacggtcacg ggccgaacga tcgccgaaaa
     3481 cctcaagagt gtgaaatgga atccgcacca ggatgtggtg cggtctgcgg acaaaccgat
     3541 caccgtcaca ggcggcgtgg ttggcttgaa ggggaatctc gcgcccgagg gcgcgatcgt
     3601 gaaggt
//
LOCUS       DATNOI010000651         4219 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2183112,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000651 DATNOI010000000
VERSION     DATNOI010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183112"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..976
                     /locus_tag="VN968_26270"
     CDS             23..976
                     /locus_tag="VN968_26270"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32772.1"
                     /translation="MSWPRLSTVVAALALVVAADRSAWSQETLKVAIPQRGAWDAGVA
                     ELGQRGGIFKKHGLDLEILYVQAGPESIQAVIGGSMDIATAAGVSAAVGTFAKGAPIR
                     IIGSEMIGAPDLYWYVPASSPIKKIEDFNGKTVAFSLTGSSSHAGLLALIAQYKLTAI
                     PTSTGTIAATITQTMTGQVDVGFGAAPFGLDLVEDGKIRIIATGGDVASLRTRTVRVN
                     LTNVNTLHKRHDAIVRFNRAYQETVAWMYSDPAALKHYGEYSNLPAKIVLRVRELIPK
                     GSMATDRVEGIDQIMADAVAAKFIPAPLTGDQVEELLQIPK"
     gene            1504..1932
                     /locus_tag="VN968_26275"
     CDS             1504..1932
                     /locus_tag="VN968_26275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32773.1"
                     /translation="MCDYSLHHVATRPARVGDKLVATRFPNSITRGFAASGEPHVAVC
                     LMPGTEIAFDQNVECEPSFGIGILPNKKIGQRLARFRQINMDNAVTHHDALEFPDGQV
                     VLVTRLCEGQCATVLQLPAGARSGTSEKAEPEAREVSVSL"
     gene            2512..2730
                     /locus_tag="VN968_26280"
     CDS             2512..2730
                     /locus_tag="VN968_26280"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HXL32774.1"
                     /translation="MRRTNRETAMPEIYVHAVKGRSLDQKRALIKDITDAVVKNFSVP
                     VEAVMVEIVESEPTAKAKGGVLFSEMRR"
     gene            2797..3552
                     /locus_tag="VN968_26285"
     CDS             2797..3552
                     /locus_tag="VN968_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL32775.1"
                     /translation="MGDLDGKTALVTGAASGIGLATVELFARRGAKVALNHLPDDPRG
                     PAAVERLRAAGFAAVGAPGDVSKAGAAEKMVAAAIDALGRLDFLANNAGTSATVAPIP
                     PAELDRLTEEFWATILATNLLGPFRCTRAAAAALKAARGAVCNTASVAGRNMAGSSMA
                     YGASKAGLINLTQNLARALAPEARANAVAPGFVETDWTRPWPAARKQAAIERSLLKRA
                     CTPADIAAAIVFLCADTSMVTAQTLVVDGGYSL"
     gene            complement(3600..>4219)
                     /locus_tag="VN968_26290"
     CDS             complement(3600..>4219)
                     /locus_tag="VN968_26290"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXL32776.1"
                     /translation="TIAIILGGILSGIFTPTESGAVACIYAFLVTMFVYRDVKWSEIP
                     KLIARVVRTVGMVMIMIGFSIAFGYMMAILRIPAKATEFFVAISSDKYTFLLYVNVLL
                     LVLGTFMDLAPMLLICTPIFLPVIKTFGIDPVHFGIIMILNLGIGLLTPPVGPTMVVG
                     CAIGRVSMEAVSRSILIFYVPMLIVLALVTYLPALSLWLPQLLLK"
BASE COUNT          814 a         1287 c         1346 g          772 t
ORIGIN      
        1 ggcgcctaga ggaggaacga caatgagctg gcctagattg agcacggtgg ttgccgcgct
       61 cgcgctggtc gtggcggccg atcgcagcgc ctggtcgcag gagacgttga aggtcgcgat
      121 cccgcagcgc ggggcatggg acgccggtgt cgccgagctc ggccaacggg gcggtatttt
      181 caagaagcac ggcctcgacc tggagatcct ttacgtccag gccgggcccg aatcgatcca
      241 ggcggtcatc ggcggcagca tggatattgc aaccgcggcc ggcgtgtcgg ccgcggtggg
      301 cactttcgcc aagggcgcgc cgattcgcat cattggcagc gaaatgatcg gtgcacccga
      361 cctttactgg tatgtccccg ccagctcgcc gatcaagaaa atcgaggatt tcaacggcaa
      421 gaccgttgcg ttctcattga ccggctcgtc cagccacgcc gggctgctcg cgctcattgc
      481 acaatacaag cttaccgcca taccgacatc gaccggtact atcgccgcga ccatcaccca
      541 gaccatgacc gggcaggtcg atgtaggctt cggcgcggca ccgttcggac ttgacctcgt
      601 ggaggacggc aagatccgga tcatcgcgac cggcggtgac gtcgcctcgc tgcgcacccg
      661 caccgtgcgc gtcaatttga ccaacgtgaa caccctgcat aagcggcatg atgccatcgt
      721 tcgcttcaat cgcgcttatc aggagacggt tgcatggatg tattccgatc ccgccgcgct
      781 caagcactac ggcgaatatt ccaatttgcc cgccaagatc gtgctgcggg tgcgcgagct
      841 tattccgaag ggaagcatgg ccactgatcg cgtcgagggc atcgaccaga tcatggccga
      901 cgcggtggcg gcgaaattta ttcccgcgcc gctgaccggc gaccaggtcg aggagttgct
      961 gcagattccg aagtgaagga gcctctgccc gagctttctc gcgcggcttg gtgcgcgttg
     1021 atcgctggga aggggagggc gtcgcctgtc ggctttcctg cgcgctcaac aagcggggtt
     1081 aaatccctcc aggaattgag cggtgggcgt ctccggcacc cgaacctgca tacctccgct
     1141 ttcgatgttg attactcaca ggaattccga ggaaacgtac gtcctgcgag cacggtcgcg
     1201 cggccaacgc cgatgacgaa ttgcctgccg cactgggcct cgttcgctgc ctttgacgaa
     1261 acgaggaacc ggttacgtca ttgccgcgtt cttcgctcac ctcctggcag ccgaaggaga
     1321 gagattgatg tggtttgaat cagctgcgag cggtcgggat ctactgctga tgagacctct
     1381 ccctgcaagg cgatgacatg gcggtaatgc gggaagtcga gacaccgaca accgaccgct
     1441 gccacgagtc tggcggcttg gctgaggagc attctggctt tcacaggaaa aggagttcct
     1501 tcgatgtgtg actatagtct gcaccacgtc gcgacccggc ctgccagagt tggggacaaa
     1561 ctggtagcaa ccaggttccc caattcgatc acgcgcggct tcgcagcgtc cggagagccg
     1621 catgttgcgg tctgcctcat gcccggcacg gaaattgcgt tcgaccagaa cgtcgaatgc
     1681 gagccgtcat tcggcatcgg catccttccg aacaagaaga tcgggcagcg gcttgcgcgt
     1741 ttccggcaaa tcaatatgga caacgccgtg acgcatcacg atgcgttgga attcccggac
     1801 ggacaggtgg tgctggtcac gcggctgtgc gagggtcagt gcgcaaccgt gttgcagctg
     1861 ccggccggcg cgcgttctgg gacgtcggag aaagcggagc cggaggctcg ggaagtttcc
     1921 gtttcgctct gatcggtcgc ttgtcgaacg ctgacgctgc cgcccgcaag gcgcggcggc
     1981 gttagaactt ggcgccgatt tcgggtgcat ccgacatcgc cgccgagcgc taaacggttt
     2041 gcctgctgaa cagcagagcc cccggcggta accggggact ctatcttgta tcacgtagaa
     2101 cgcgtgcgaa gacgaacgtt agcgctcggg aaatgtgact agcgtctcga acttatagcc
     2161 gcacgcttcg cactcccaga gatgccgaac acggtgccgg tccaggtact cggaccattc
     2221 cggcaggaag attgcttccc cgcattgggc acagtgattg agcagcttgg tctgaacgaa
     2281 ctttcggggg ctcgcggtgc gaagctccat ggaggcgtcc tccccaccat gctccccctc
     2341 cccactgtgc ggcatgctag cgcaactgcc gctgaggtga agcaggagcg ctgacctaac
     2401 gcggtcagcg cccgtcaccc cgcgagggcc tcttcggctt cttgcggtga gccgtcccgc
     2461 gttgcgcaga ggacgtcttc ctatgtccgg cacagtcgct atagtgcccg ggtgcgacgc
     2521 acaaataggg agaccgccat gcctgaaatc tacgtccacg ccgtcaaggg ccgctcgctc
     2581 gatcagaaac gcgcactgat caaggatatc accgacgcag tggtgaaaaa tttctccgtt
     2641 ccggtcgagg cggtgatggt ggaaatcgtg gaatccgagc cgaccgccaa ggcaaaaggc
     2701 ggtgttttgt tcagcgagat gcgtcgctga gccgggggcg cggccggcag tgtttcgccc
     2761 ggcaacgccc gggcgaaact tttttcggag ccaaccatgg gcgatcttga cggcaagacc
     2821 gcactggtca cgggcgcagc atcgggcatc ggcctcgcta ccgtcgagct gttcgcccga
     2881 cgcggcgcca aggttgccct caatcacttg ccggatgatc cccgcggccc cgcggccgtc
     2941 gaacggctgc gcgccgccgg ctttgccgcg gtcggcgcgc ctggcgatgt atccaaagct
     3001 ggcgctgcgg aaaagatggt cgccgctgca atcgacgcgc tgggccggct cgacttcctc
     3061 gccaacaacg ccggcacctc ggcaacggtc gcaccgatcc cgccggcgga gctcgaccgc
     3121 ctgaccgagg aattttgggc aaccattctt gccaccaacc tgctcgggcc gttccgctgt
     3181 acgcgcgccg ccgcggcggc tctcaaggca gcacgcggtg cggtttgcaa taccgcgtca
     3241 gtcgccggtc gcaatatggc ggggtcaagc atggcctatg gcgcgagcaa ggccggcctc
     3301 atcaatctga cccaaaactt ggcgcgcgcg ctggcgcctg aggcgcgcgc aaatgccgtg
     3361 gcgcccggat ttgtggagac cgactggacc cggccttggc cggcagcgcg caagcaggcg
     3421 gcaatcgagc gctcgctgct caagcgagcc tgcacacctg ccgatatcgc cgcggcgatc
     3481 gtatttcttt gcgccgatac cagcatggtg acggcgcaaa cgctggtggt cgatggcggg
     3541 tactctttgt gacgcgcctg gatgcttgct cgcgcgtcgc aggttcgccg ccagatcggc
     3601 tatttgagca gcagctgcgg cagccaaagt gacagtgccg gcaggtaggt caccagtgcg
     3661 agaacgatca gcatcggcac atagaagatc aggatgctgc gcgacaccgc ctccatgctc
     3721 acgcggccga tagcgcatcc gaccaccatg gttggtccca cgggcggagt gagcaggccg
     3781 atgccgagat tgaggatcat gatgatcccg aagtgtacgg ggtcgatacc gaaggtcttg
     3841 atcacgggca gaaaaatcgg cgtgcagatg agcagcattg gcgccagatc catgaatgtg
     3901 ccgagcacca gcagcaaaac attcacgtag agtagaaatg tgtacttgtc gctcgagatc
     3961 gcgacgaaga actccgtggc tttggctggg atgcgcaaga tcgccatcat gtagccgaac
     4021 gcgatcgaga agccgatcat gatcatgacc atgccgacgg tgcgcacgac gcgtgcgatc
     4081 agcttcggaa tctcgctcca tttgacgtcg cggtagacga acatcgtgac aaggaaggca
     4141 tagatacagg cgactgctcc ggattcggtc ggcgtgaaga tgccggacag gatgccgccg
     4201 aggatgatcg cgatcgtga
//
LOCUS       DATNOI010000652        10104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2292642,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000652 DATNOI010000000
VERSION     DATNOI010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2292642"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VN968_26295"
     CDS             complement(<1..196)
                     /locus_tag="VN968_26295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HXL32777.1"
                     /translation="MSAFHREKVLSVRHWTDTLFSFTATRNSGFRFQNGQFAMIGLEV
                     DGRPLMRAYSMASANHEEELE"
     gene            517..1512
                     /locus_tag="VN968_26300"
     CDS             517..1512
                     /locus_tag="VN968_26300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32778.1"
                     /translation="MRSLSEFRSGKPRFYPDQSVYAEYACAEFGLSFQDIDGGSGLLF
                     RVVSKTRSVHFGAGRCSWYPQNNATAATLASDKYFTDRILEHAGVATLGGEYFFLHER
                     RRSQRAPGHEREDAFAYLRKLGGTAFVKPLSGSRGDFAQIIGGETLLAGYLDEVSQYY
                     DSILIQPIATGNEYRIFLLDDEIVYSARKCPPFVSGDGARSIGELLIAHNEALRSRGL
                     SPVSVSPDASLGIVPAKGERWEIPGRMNLSAGGTMVLEAPPSDAALVLARKAARALGL
                     RVAAVDMFADIGRDPDAMRVIEVNSNPSIRLLEESGRSDLILKIWRHTFMAMGLL"
     gene            1512..2828
                     /locus_tag="VN968_26305"
     CDS             1512..2828
                     /locus_tag="VN968_26305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HXL32779.1"
                     /translation="MFDLPKYGNGVCLARLADLLDALAVDRARLSRISVVVTGSNGKG
                     STAAFCAGIARACGLRTGLFTSPHLYRLNERVRVDGADIGDDDLARLKLRIEAAIAEI
                     SWRTGEQFGAFEALFALACLYFQERNCGFAVFEAGIGGRYDPVRLVGAHLTCVTSVDY
                     EHVELLGNTLQLIASDKSDACASGGTIVYGENCRNLRPHLIEYNRNRAIASLFVRGEI
                     GISGESLSATGQQFDFQFGAHEFRSLEVSLLGSFQFNNAAIAVALFLLWLAHAQPGET
                     PDKIESAVLSGLREARWPGRLEVIKQDPLTVIDVGHTPDGIRQSLASLKAIYGREGWI
                     LVVGVSFDKNADEIVGALAPSFGTIICTAAHHKGADASMIAAAVRKANPQATIHIAAA
                     IEEAVSISQRLAASQNRKIYVAGGLFLAIEYAVVASGGRAQDLRFF"
     gene            complement(2956..3885)
                     /locus_tag="VN968_26310"
     CDS             complement(2956..3885)
                     /locus_tag="VN968_26310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL32780.1"
                     /translation="MGYPPKRCVPAASLVAALVLLTTAAGAQTGGSEGLSPTLANIKK
                     AHVVRLGYRESSPPFSFLDQANRPIGYSLELCEAIVDEIGVEVDDANLKIEYVKVTSD
                     DRIPAVVQNKIDLECGSTTANAERGKQVAFSPLIFVAGTKLMVPKASTVAGAADLKGK
                     TVVVTKGTTNEQAMHAVDKKFALGLNIIASPDHEQSYQMLVDQKADAFATDDILLYGL
                     IARHKAQGKFRVTGEYLSYDPYGIMFRKGEPQLAAAVERTFRKLGSNRDLIPLYNKWF
                     VARLPTGEKLNVAISPQLEEAFKALNDTEGTSN"
     gene            complement(3896..5197)
                     /locus_tag="VN968_26315"
     CDS             complement(3896..5197)
                     /locus_tag="VN968_26315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011082935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HXL32781.1"
                     /translation="MSNRFTQYILIAMALGIVMGTLVFNYLPDIRADIAADVNLIAML
                     FLRLIKMIIAPLVFATLVGGIAHMGTGSKLGRIFAKTMGWFVSASFVSLLLGLVMVNL
                     LQPGANFPGTLPDKLQSTGLPVSAFSIEKFLTHLIPTSIADAMAQNEILQIVVFAVFF
                     SVALGAMPERSKQILSVIDDLGHIMLKVTAYVMLFAPLAVWAAIMATVSKNGLGVLWK
                     LIVFMGGFYLSLLILWGILVIVGFIVIGPRYSHLLRLIREPLMIAFSTASSEAAYPKT
                     LEGLNRFGASSRISSFVLPLGYSFNLDGTMMYCTFASIFIAQTYHIEMPLATQLAMLA
                     TLMITSKGVAGVPRASLVVIASTLSQFGIPEAGLLMIMGIDTFLDMGRSATNVIGNSL
                     ATAVVAKWEGELSPEHALGPDDVVPPDMIPGELPGMQAGHA"
     gene            5406..6407
                     /locus_tag="VN968_26320"
     CDS             5406..6407
                     /locus_tag="VN968_26320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HXL32782.1"
                     /translation="MNDTVNRQVLLVEKPAGKLGPEHFKMSKSGIPEPKDGEVLVRVR
                     YISLDAANRAWMHGATYRAAVEANTVMAGGGIAEVVSSKAPGLAPGDIVFGDTGWQDY
                     AAVPAKHLNRMPKIEPMTHLLSVYGIAGLTAYFGLLDVGKAKAGETVVVSAAAGSVGS
                     IVGQIAKIKGCRAVGIAGGKDKCNWLTSELGFDAAVDYKDGAVFKALKAAAPGGIDVY
                     FDNVGGDILEACLSQMNNRGRIACCGAISQYDGVPSATGPRGVPGLIVVKRLTMQGFI
                     VMDYMDQRDQALSDLQSWVKAGKLKVQEDVINGLENTPKALIGLLAGENRGKRMVRV"
     gene            6471..7214
                     /locus_tag="VN968_26325"
     CDS             6471..7214
                     /locus_tag="VN968_26325"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXL32783.1"
                     /translation="MVGTLRLAHPGDPRRGHIGASVVQAGGPPVNRFTLYGSPHSLPT
                     YKVALMLRLSGAAFSFRYVSYQKNMHKTPEFLALSRWGQVPVLVDNGRPHFQSAAIVE
                     HLAETLDRFRGPDSAARQAVREWLYWDVDVLFPPIFNSYAVQLGQRNLLPINVDPVIA
                     EYHRQRSGAALSVLDSHLADRSHLCADEPTIADLFCYADVAFAEICAFDLRHWTNLTL
                     WAKKVTALAGFQAPFALLAMEDAEFSAGT"
     gene            complement(7341..>10104)
                     /locus_tag="VN968_26330"
     CDS             complement(7341..>10104)
                     /locus_tag="VN968_26330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXL32784.1"
                     /translation="ILLFVTLTSPNKTYDSLFLSNYATINIRDELSRLPGVGNVTVFG
                     AGQYSMRVWLDPNKLQVRNLMPQDVISAIQQQSQQVTAGQIGMPPTPPGQAFQLTLNV
                     NGRLDDKDQFEDIIVKTGNSGDVTRVRDIGWVELGAQTYSQTFSLNNKPATGIGVFQS
                     PGANALEVEQAVQKKMAALAKGFPQDVRYDTPFDTTKFVSESIKEVYKTLIEAGLLVL
                     LVILIFLQDWRAMLVPATTVPVTIIGAFAAMAALGFTVNISTLFAIVLAIGIVVDDAI
                     VVVEGAAHNIERGMSGHDAAISAMDALFAPIIGITLVLISVFLPSAFLPGLTGRMYSQ
                     FALVIAATALLSAINAATLKPTQCALWLRRPAPPEQRNFFYRGFNAVYDRLESGYARL
                     IGRLVAHSNVSVIVALILIGVGGYGLSRVPTGFIPIEDQGYLLVAAQLPDGAALDRTQ
                     RVLDQISELAGKTPGVDQVISIAGISALDNSSSLANAGVAYLVLKEWSARGPGQDLRS
                     LFVGLNEKMAAIPEAQILVIPPPPIQGIGNAAGFAMQVQLRDGNSDFGKLQAIAGAIV
                     ANAQTQSALQRVSSSFRSMVPQFDVDVDRIKTQTLQVTTDQIFSTLSSYTGSTYVNQF
                     NKFGRTFQVYTQADAQFRLTLRDIENLMVRNSQGAMIPLGTVAKITPAVGPSLISLYN
                     LYPSATVIGLPATGYSSGQSMTLMEEIAAKTLPPGTGYEWTAMSYQEKVVGGQIYLAF
                     GLALLLVYLVLAGQYESWYAPISVILAVPLSLLGPMIVLTGLRIENNLYTQIGIILLI
                     ALSAKNAILIVEVALEHHVRDRKPLLESAIEAARARFRPILMTSFAFILGVVPLVLAT
                     GAGASARKSIGITVFSGMIASTCLAVLFVPAFFVVIQRFENWLKQRKGKAAAPQPIPQ
                     APSATP"
BASE COUNT         1987 a         3034 c         3140 g         1943 t
ORIGIN      
        1 attccagttc ttcctcgtga ttggcgctcg ccatgctgta ggcccgcatc aatggacggc
       61 catcgacctc gagcccgatc atcgcgaact ggccgttctg gaatcgaaag ccggaattgc
      121 gggtggcggt gaagctgaac agcgtatcgg tccagtgccg gacagaaaga accttctctc
      181 gatggaatgc gctcatgtgt tttgtttttc cgattgcctg acagtagaac gaacgagttg
      241 cgcggctcgc gaagcggcga aaacccctgc gacgattgag gatttagcag ctgacctaca
      301 ctgatattag agatagtcaa tgaggtccga ggttcaattg agagctcaaa atagcgccgg
      361 tttttgttgc caaaaaagtc tttgctgttt atcggcgctt ctgagcaatt aacttgctga
      421 aatccgccgg catcgagtag ttcattgccg tgctcggata aggatttggt tagagatatt
      481 tgatcgaaaa ccgaatgcca gatcgccgga tattgcatgc gaagccttag cgaatttcga
      541 tcgggaaagc cgcgcttcta tccggaccaa tcggtctacg cggaatatgc ctgcgctgaa
      601 tttggcctgt cgtttcagga catcgacggc ggatcagggc ttctgttcag ggtcgtatcg
      661 aagaccagga gcgttcattt cggcgccggg cgatgttcct ggtatccgca aaacaatgcc
      721 accgctgcaa cgctggcgtc ggacaaatat tttaccgatc ggattctgga gcatgccggc
      781 gtagcaaccc tgggcggcga atatttcttc ctgcatgagc ggcgccgcag ccagcgcgcc
      841 ccgggccacg agcgcgaaga cgcgttcgca tatttgagaa agctcggcgg cactgccttc
      901 gtcaaaccgc tgtcgggatc gcgcggcgat tttgcccaaa tcattggcgg cgagacactg
      961 cttgcaggat acctggacga ggtttcgcaa tactacgatt cgatattgat ccagccgatt
     1021 gcaacgggaa acgagtaccg gattttcctg ctcgacgacg agatcgtgta ttccgccaga
     1081 aaatgtccgc cgtttgtgtc aggcgatggt gcgcgttcga ttggcgagct gttgatcgcg
     1141 cataatgaag cgttgcgctc gcgcggtctt tcgccggttt ccgtaagccc cgacgcttcg
     1201 ctcggcatcg tgccggccaa gggcgagcgc tgggagattc ccggacggat gaatctgagt
     1261 gcgggcggaa cgatggtgct cgaagctcct ccgtccgatg ccgcgctcgt actggcgagg
     1321 aaggccgcgc gagcgctcgg gctccgcgtc gccgccgtgg acatgtttgc cgatatcggc
     1381 cgagatccgg atgcgatgcg ggttatcgaa gtaaactcca acccctcgat ccggctgctg
     1441 gaagaatccg gccgcagcga cctgatcctg aaaatctggc gccacacatt tatggcgatg
     1501 ggactacttt agtgtttgat cttccgaaat acggcaacgg ggtttgtctg gcgcggctgg
     1561 ccgacctgct cgatgctctt gcggtggacc gggcaaggct ctcgcgtatt tcggtcgtgg
     1621 tgacggggtc caatggcaaa ggctctaccg ccgcgttctg tgccggtatc gcccgcgcct
     1681 gcggcttgcg aaccggattg ttcacttcac cgcatctgta tcgtttgaac gaacgggttc
     1741 gggtcgacgg cgctgatatc ggcgatgacg acctggcgcg gctgaagttg cggatcgaag
     1801 cggctatcgc cgaaatctcg tggcgtaccg gagaacaatt cggagccttc gaggccctgt
     1861 tcgcgctggc ctgcctgtat tttcaggagc gcaattgcgg tttcgcggtg ttcgaggccg
     1921 gcatcggcgg ccgttacgat ccggtccgcc tggtcggcgc gcaccttacc tgcgttacgt
     1981 ccgtcgacta cgagcatgtc gaactgctcg gcaatacgct gcaactgatc gcttccgaca
     2041 agagcgacgc ctgcgcatcc ggcggcacca tcgtctacgg cgagaattgc cgaaacttga
     2101 ggccgcacct catcgaatat aatcgcaatc gcgcgatcgc gtcgctgttt gtccgcggcg
     2161 agattggcat atcgggcgaa tccctctcgg cgaccgggca acagttcgat ttccagttcg
     2221 gggctcacga atttcggtcg cttgaagtga gcctgctcgg ctcctttcaa ttcaacaatg
     2281 ccgcgatcgc ggttgctctc ttcctgctct ggctggcgca tgcgcaaccc ggagaaactc
     2341 ccgacaaaat cgagtccgcc gtgctgtcag gcctgcgcga ggcacgctgg cccgggcgcc
     2401 tcgaggtcat aaaacaagat ccgctcaccg tgatcgacgt cggccacacc cccgacggca
     2461 tccggcagtc gctcgcaagc ctcaaggcga tttatggccg agagggctgg attctggtgg
     2521 tcggcgtttc cttcgacaag aacgccgacg agatcgtggg cgcgctggcg ccgtcgttcg
     2581 gcacgatcat atgcaccgcg gcgcatcaca agggcgcgga cgcaagcatg atcgccgccg
     2641 cggtgcgcaa ggccaatccg caagcaacga ttcacatcgc cgcggccatc gaggaagccg
     2701 tcagtatcag ccaaaggctc gccgcgtcac agaatcgaaa aatatacgtg gccggcggat
     2761 tattcctggc gatcgaatac gccgttgtcg ccagcggcgg ccgcgcgcag gatttgaggt
     2821 ttttctgacg aagcctcacg gcgccgcacc tcatcctgag gagcggcctc ttggccgcat
     2881 ctcgaaggat ggggcctcat ggttcgagac ggcgcaaggg cgcctcctca ccatgaggat
     2941 agaaatcgtg ccagcctagt tgctagtccc ctcggtatcg ttgagcgcct taaaagcttc
     3001 ctccagctgc ggcgagatcg cgacgttgag cttctcgccg gtcggcaggc gcgcgacgaa
     3061 ccatttgttg tagagcggaa tcaggtcgcg attcgaaccg agcttgcgga aggtacgttc
     3121 caccgcggca gcgagttgcg gctcaccctt gcggaacatg atgccgtagg gatcgtagga
     3181 cagatattcg ccggtaacgc gaaacttgcc ctgcgccttg tgccgcgcga tcaggccgta
     3241 aagcagaatg tcgtcggtcg cgaacgcatc ggccttttga tccaccagca tctgataaga
     3301 ttgctcgtga tcgggcgagg caatgatgtt gaggccgagt gcaaatttct tgtcgacggc
     3361 atgcatcgcc tgctcgttgg tggtgccttt tgtcaccacc accgtcttgc ccttcaaatc
     3421 ggccgctccc gcgacggtcg aggccttcgg caccatcaac ttggtgccgg ccacgaatat
     3481 cagcggcgag aacgccacct gtttgccgcg ctctgcattg gccgtggtcg atccgcattc
     3541 gaggtcgatt ttgttctgca ccaccgccgg gatgcggtcg tcggatgtga ccttgacgta
     3601 ttcgatcttc aggttggcat cgtcgacctc gacaccgatc tcatcgacga tcgcctcgca
     3661 gagctccagg ctgtagccga tcggccggtt ggcctgatcg agaaacgaaa acggcggcga
     3721 gctctcgcga tagccgagac gcacgacatg cgccttcttg atattggcaa gcgtcgggct
     3781 cagcccttcg ctgccgccgg tctgggcgcc cgcggccgtg gtcagcaaaa ccaacgcggc
     3841 caccaggctt gccgccggta cgcatctctt gggagggtag cccatagctc gcctcctatg
     3901 cgtggccggc ctgcattccc ggcaactcgc cggggatcat atccggcgga acgacgtcgt
     3961 cgggaccaag cgcgtgctcg ggcgaaagtt cgccctccca cttcgccacc acggcggtcg
     4021 cgagcgagtt gccgatcaca ttggtggcgc tgcgtcccat gtcgaggaac gtatcgatac
     4081 ccatgatcat caacaggccc gcttcgggaa tgccgaactg gctcagcgtc gaggcgatca
     4141 ccaccagcga agcgcgggga acgccggcga cacctttcga tgtgatcatc aaggtcgcaa
     4201 gcatcgcaag ctgggtcgcg agcggcattt cgatgtgata ggtctgcgcg atgaagatgc
     4261 ttgcaaacgt gcagtacatc atggtgccgt cgagattgaa cgaatagccg agcggcagca
     4321 cgaaactcga gattcgcgag gatgcgccaa agcgattgag gccctccagg gtcttcggat
     4381 acgcggcttc cgaactcgcc gtcgagaacg cgatcatcag gggttcgcgg atcagccgca
     4441 gcagatgact gtatctcggc ccgatgacga taaagccgac gatgacgagg atgccccaca
     4501 ggatcagcag cgagagatag aagccgccca taaagacgat cagcttccac aatacgccga
     4561 ggccgttctt cgacaccgtc gccatgatcg cggcccacac cgcaagcggc gcaaacagca
     4621 tcacataggc tgtgaccttc agcatgatgt ggccgaggtc atcgatcacc gaaagtatct
     4681 gcttggagcg ttccggcatg gcgccaagcg cgaccgagaa gaacaccgcg aaaaccacga
     4741 tctgcagaat ttcgttctgc gccatcgcat ccgcgatcga ggtcgggatc aaatgggtca
     4801 ggaatttttc gatcgagaat gccgacaccg gcaatcctgt ggattgcagc ttgtccggca
     4861 gggtgcccgg aaaattcgca cccggctgca acagattgac cataaccaat ccaagcagca
     4921 gcgagacaaa cgacgcgctg acgaaccagc ccatggtttt tgcgaagata cgcccgagtt
     4981 tggagccggt tcccatgtgg gcgatgccgc cgaccagggt cgcgaacacc agcggcgcga
     5041 tgatcatctt gatcaggcgc aggaacagca tcgcgatcag gtttacgtct gcggcgatat
     5101 ccgcccggat gtcgggaaga tagttgaaga ccagtgtccc catcacaatg ccgagcgcca
     5161 tcgcgatcag aatgtattgt gtaaacctgt tggacatttt cttaccccca aatttccgac
     5221 ggtcccgagt ttgccagatt ccgggagcga cgcaacacgc ttcaaacgtc agtgcctttg
     5281 ccacaaactt cccattgtgg aatgagcgcc cgccgactag cgtcggaagc agcgcagccg
     5341 ggcagttgag cgtcgcgacc gcggccggct gcatcaagca tcgacatatc aagggaggga
     5401 acgccatgaa cgacaccgtc aatcgtcagg tcttgctggt tgagaagcca gccgggaaac
     5461 ttgggcccga gcatttcaaa atgtccaaaa gcggcattcc cgagccgaag gacggcgaag
     5521 tgctggtgcg ggtgcgctac atctcgctgg acgcggccaa ccgggcctgg atgcatggcg
     5581 cgacctatcg cgcggcggtc gaggccaaca ccgtgatggc cggcggcggc atcgccgaag
     5641 tcgtgtcctc gaaagcaccg ggactggcgc cgggcgacat cgtgttcggc gataccggct
     5701 ggcaggatta tgccgctgtc ccggcaaaac atctgaacag gatgccgaaa atcgaaccga
     5761 tgacgcatct cctgagcgtt tacggcatcg ccggcctcac cgcctatttc gggctgctcg
     5821 atgtcggcaa ggcgaaggcc ggcgagaccg tggtggtctc ggccgccgcc ggttcggtcg
     5881 gatcgatcgt cggccagatt gcaaaaatca agggatgccg cgcggtcggt atcgccggcg
     5941 gcaaggacaa gtgcaactgg ctgacctcag agctcggctt cgatgccgcg gtcgactaca
     6001 aggacggcgc ggtgttcaag gcgctgaagg ccgcggcgcc tggtggcatc gacgtctatt
     6061 tcgacaatgt cggcggcgat attctcgaag cctgcctttc gcagatgaac aaccgcggcc
     6121 gcatcgcctg ctgcggcgcg atctcgcaat atgacggcgt gccgtcggcg accgggccgc
     6181 gcggcgtgcc cggcctgatc gtggtcaagc gcctcaccat gcaagggttc atcgtgatgg
     6241 actacatgga tcagcgcgat caggcgttga gcgacctgca atcctgggtc aaagccggca
     6301 aattgaaagt ccaggaggac gtgattaacg gcctggaaaa tacgccgaaa gcgctgatcg
     6361 gactgcttgc gggcgaaaac cgcggcaagc gcatggtcag ggtgtagggc atacggggaa
     6421 aaggcgctct tttgcgccgt gccccgcttt gatctatcgg atcgctctga atggtgggca
     6481 cgctacgctt ggcccaccct ggggatccaa ggcgtggaca cataggagcg tcggtcgttc
     6541 aggcaggagg accacccgtg aatcgcttca cgctctatgg cagtccgcat tcgcttccga
     6601 cctacaaggt agcactgatg ctgcgcctgt ccggcgcagc gttttcgttt cgctacgtaa
     6661 gctaccagaa gaatatgcac aagacgcccg aattcctggc gctgtcgcgc tggggccagg
     6721 tgccggtttt ggtcgacaac ggtcgacccc actttcaatc ggcggccatc gttgagcatc
     6781 tggccgagac actcgatcgg tttcgggggc ccgattcggc ggcgcgccag gccgtgcgcg
     6841 agtggctgta ctgggacgtc gacgtgctgt ttccaccgat cttcaatagc tacgccgttc
     6901 aactcggtca gcgaaacctg ctgccaatca acgtcgatcc ggtgatcgcc gaatatcatc
     6961 gccagcgctc cggggcggcg ctgtcggtcc tggattccca tctcgctgat cgcagccatc
     7021 tctgcgcaga tgaacccaca atcgccgacc tgttctgcta cgcagacgtc gccttcgcgg
     7081 aaatctgtgc gtttgacctg aggcattgga ccaacctcac tctctgggca aaaaaagtga
     7141 ccgcgctggc gggtttccag gcccccttcg cattgctggc catggaggac gccgagttct
     7201 cggcggggac ttgatcgacc agcaaagctg tcttgcgcct tgcccaacat ctacccccga
     7261 tcggtatctt gaatggcggg cacgcggagc ccgtcatcgg acgcgtattc gcgcgagcgt
     7321 tggctcgccc attctgcgaa ctatggagta gcgctcggcg cctgcggtat cggttgcggc
     7381 gccgccgctt tgcccttgcg ctgcttgagc cagttctcga accgctggat cacgacgaag
     7441 aacgccggca cgaacaagac cgcgaggcag gtcgaggcga tcatgcctga aaacacggtg
     7501 atgccgatcg acttgcgggc gctggcgccg gcgccggtcg caagcaccag cggcaccacc
     7561 ccgaggatga aggcaaacga cgtcatcagg atcggccgga accgcgcccg cgccgcctcg
     7621 atcgccgatt cgagcagcgg cttgcggtca cgcacgtggt gctcgagcgc gacctcgacg
     7681 atcaggatgg cgttcttcgc cgacagcgcg atcaacagga tgatgccgat ctgggtgtag
     7741 aggttgttct cgatccgcaa gccggtgagc acgatcatcg ggccgagcag cgacagcggc
     7801 accgccagga tcaccgagat cggcgcgtac cagctctcat attgcccggc cagcacgagg
     7861 tagaccagca gcaaggccag gccgaatgcc aggtagatct gaccgccgac gaccttctcc
     7921 tggtacgaca tcgccgtcca ttcatagccg gtgcccggcg gcagggtttt ggcggcgatc
     7981 tcttccatca gcgtcatcga ctggccggag ctatagccgg tggccggcag gccgatcacc
     8041 gtggcggatg gatagagatt gtacaggctg atgagcgatg gacccaccgc cggcgtgatc
     8101 ttcgccaccg tgccgagcgg gatcatggcg ccctggctgt tgcgcaccat caggttctcg
     8161 atatcgcgaa gcgtcagccg aaactgcgca tccgcctggg tgtagacctg gaaggtgcgg
     8221 ccgaacttgt tgaactggtt gacgtaggtc gagcccgtat aggaggacag cgtcgagaaa
     8281 atctggtcgg tcgtgacctg cagggtctgg gtcttgatcc ggtcgacgtc gacgtcgaac
     8341 tgcggcacca tcgagcggaa tgacgagctg acccgctgca gggcgctttg cgtctgtgca
     8401 ttggcgacga tggcgccggc gatagcctgc agtttgccga aatcggaatt gccgtcacgg
     8461 agctggacct gcatggcaaa gccggcggcg ttgccgatgc cctgtatcgg cggcggcggg
     8521 atcaccagaa tctgcgcctc cgggatcgct gccatcttct cgttaagccc gacgaacaac
     8581 gatcgcagat cctggcccgg accacgcgcg ctccactcct tcagcaccaa ataagcgacg
     8641 ccggcgttgg caaggctcga agagttgtcg agcgccgata tgccggcgat gctgatcacc
     8701 tgatcgacgc ccggggtctt gccggccagt tcagagatct ggtcgagcac gcgctgggtg
     8761 cggtcgagcg cggcgccgtc gggcagttgc gcggcaacca gcagatagcc ctggtcctcg
     8821 atcgggataa agcctgtcgg cacccgcgac aggccatagc cgccgacgcc gatcaggatc
     8881 agcgcgacga tgaccgagac attgctgtgc gccaccaggc ggccgatcag ccgggcatag
     8941 ccgctctcta ggcggtcata gaccgcgttg aagccgcgat agaagaaatt gcgctgttcc
     9001 ggcggcgccg gccggcgcag ccacaatgcg cactgcgtcg gtttcagcgt ggcggcattg
     9061 atggcgctga gcagtgcggt cgcagcgatc accagcgcga attgcgagta catcctgccg
     9121 gttaatcccg gcaggaaggc agagggcagg aacaccgaga tcaacaccag cgtgatgccg
     9181 atgatcggcg cgaacagcgc gtccatggcg ctgatggcgg cgtcatggcc ggacatcccg
     9241 cgctcgatat tgtgcgcggc gccctccacg acgacgatgg catcgtccac cacgatgccg
     9301 atcgcgagaa cgatcgcaaa caaggtcgag atgttgaccg tgaagccgag cgccgccatc
     9361 gcggcgaagg cgccgatgat ggtgaccgga acggtcgtcg ccggcaccag catcgcgcgc
     9421 cagtcctgca ggaagatcag gatcacgagg agcacaagca ggccggcctc gatcagcgtc
     9481 ttgtagacct ccttgatcga ttccgagacg aacttggtgg tgtcgaacgg cgtgtcgtac
     9541 ctgacgtcct ggggaaatcc cttcgccagc gccgccatct ttttctgcac cgcctgctcg
     9601 acctcaagcg cgttggcacc gggcgactgg aacacgccga tgccggtcgc cggcttgttg
     9661 ttgagcgaaa acgtctggct ataggtctgg gcgccgagct cgacccagcc gatgtcgcgc
     9721 acgcgggtga catcgccact attgccggtc ttgacgatga tgtcctcgaa ctggtccttg
     9781 tcatcgagcc ggccgttgac gttgagcgtc aactggaaag cctgccccgg aggcgtcggc
     9841 ggcatgccga tctggcccgc ggtcacctgc tggctctgct gctggatcgc ggaaataacg
     9901 tcctgcggca tcaggttgcg cacctgcaat ttgttcggat cgagccagac ccgcatcgaa
     9961 tattggccgg cgccgaacac ggtgacgttg ccgacgccgg gcaaccgcga cagctcgtcg
    10021 cgaatgttga tggtggcgta gttcgacagg aacaggctgt cataggtctt gttcggcgac
    10081 gtcaatgtca cgaacaacag gatc
//
LOCUS       DATNOI010000653         3422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2395513,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000653 DATNOI010000000
VERSION     DATNOI010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2395513"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VN968_26335"
     CDS             complement(<1..525)
                     /locus_tag="VN968_26335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HXL32785.1"
                     /translation="MAMHQINPQGAKLAALDPIWDRVRKEAEDIVRREPELASFIYST
                     VLHHERLEDSVVHRISERLDHSALSGDLIRQAFDEALRDDPDLGNIFRADMVAVFDRD
                     PATSRFIDPLLYFKGFHALQTHRLAHWLYHKGRKDFASYLQSRSSAVFQTDINPAAKI
                     GRGIFLDHATGFVVG"
     gene            complement(744..1496)
                     /locus_tag="VN968_26340"
     CDS             complement(744..1496)
                     /locus_tag="VN968_26340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL32786.1"
                     /translation="MPSFHNGAVEIAYLDEGEGDPILLVHGFASTKNVNWVYPAWVSE
                     LKKNGRRVIALDNRGHGDSAKLYDAREYEIATMAGDVSALMDHLDIERADIMGYSLGS
                     RMTAVLAQSQPQRLRSAILGGIGIGLIEGGGPGENVAEALEASSLDDVTDPVGRTFRA
                     FADQTRSDRLALAACLRGSRRLMTKDEAAGITVPVLIAVGTSDEIAGSAQALGKIIPG
                     ARVLDIPNRDHMRAVGDKVYKTGVLEFLSDRP"
     gene            complement(1552..2694)
                     /locus_tag="VN968_26345"
     CDS             complement(1552..2694)
                     /locus_tag="VN968_26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32787.1"
                     /translation="MKRARIGSALVGTTVCAVACALLWPHARDAGAILAAQDDPAALS
                     DIQLNSALRDNKPVIAANIEAALAAKDADLASSFVELAREKGIPVADELSRRVSEAVT
                     EENSSSHFAKRFATGLVTGNADDVASLSGTVAGDLFVFGDIRDVVREGKHLAMGEDTD
                     RLILGLATAGLAVTAVTYISVGGAVPVHAGLTLVKDARKVGRLGEGLTRWASRSAREV
                     VDAPMLQHAVASGSVMRPGETMTAIRAAFRAEKAGGLVRLAKDVGRVSEKAGTRGALE
                     TLKIAEGPKDVARAARLAESKGGQTRAILKLLGRGALLLAVGAFNLSLWVFGALLALF
                     GFLSSIKATTERLTMSWLLRKKARRLRKQAAAMSAHQALVAAAAPG"
     gene            3074..3319
                     /locus_tag="VN968_26350"
     CDS             3074..3319
                     /locus_tag="VN968_26350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HXL32788.1"
                     /translation="MSDHIVPHFHNDAGVSVIEIGSQEFMCVGANPPFDHPHVFLDLG
                     NDNEIICPYCSTLYRYAADLKAGEARPPECVLKDKVA"
BASE COUNT          649 a         1113 c         1030 g          630 t
ORIGIN      
        1 gccgaccacg aaaccggtgg cgtgatcgag gaagatgccg cggccgatct tggctgcggg
       61 attgatgtcg gtctggaaca ccgccgagga gcggctctgc agataagacg caaaatcctt
      121 gcggcccttg tgatagagcc aatgcgccag acggtgggtc tgcagcgcgt ggaagccctt
      181 gaagtaaagc aacggatcga tgaagcgcga tgtcgccgga tcgcggtcaa ataccgccac
      241 catgtcggca cggaagatgt tgccgagatc ggggtcgtcg cgtagcgcct cgtcaaaggc
      301 ctggcggatc aagtcgcccg acagcgccga atgatcgaga cgctcggaga tgcgatgcac
      361 caccgaatcc tccaaccgtt cgtgatgcag cacggtggaa tagataaagg aggcgagttc
      421 cggctcgcgg cggacgatgt cttccgcctc cttgcgcacg cgatcccaga tcggatcgag
      481 tgccgccagc ttcgcaccct gtggattgat ctgatgcatg gccatgtgct gctctcgcaa
      541 gttcgtttga tctcgacggg cattatagca cggcgagcgg acggctgtcc ttaagccact
      601 ttagcaaaag tgaaccccga ccctggcgtc gagcgtgata taacgtattg atataacacg
      661 cattatctac tccggccgaa gtccgcttgg gctgaaagcg tgggttcaaa gtggtcggca
      721 atcccgggaa ttcaagccga cgatcagggg cgatccgaca aaaattccag caccccggtc
      781 ttgtagacct tatcgccgac cgccctcatg tggtcacggt tgggaatgtc gagcacccgc
      841 gcaccgggga tgattttgcc aagcgcttgc gccgagccag cgatttcgtc cgaggtgccg
      901 accgcgatca gcactggcac ggtgatcccg gcggcttcgt cttttgtcat caagcgccgc
      961 gagccgcgca ggcaggccgc cagcgccagg cgatcggagc gggtctggtc ggcaaacgcc
     1021 cggaacgtcc gccccaccgg atcggtgacg tcgtcgagcg acgacgcctc cagcgcctcg
     1081 gcgacgttct cgccgggacc gccgccttcg atcaggccta tgccgatgcc gcccaaaatc
     1141 gccgagcgca gccgctgcgg ctgactttgc gccagcacgg ccgtcatccg tgatcccagc
     1201 gaatagccca tgatgtccgc gcgctcgatg tccaggtgat ccatcagcgc gctgacgtcc
     1261 ccggccatgg tggcgatctc atactccctg gcatcgtaca gcttggctga atcgccatgg
     1321 ccgcgattgt cgagggcgat gacgcggcgg ccgtttttct tcagttccga aacccaggcc
     1381 gggtacaccc agttcacatt cttggtcgac gcaaagccat gcacgagcag gatcggatcg
     1441 ccctcgcctt cgtcgaggta ggcaatctca acagcaccgt tgtgaaaact cggcatcggt
     1501 cattccatat tttttggcaa gatttgggtg atcggaaaaa acgacgggct tttaaccggg
     1561 cgcggcggcg gccaccagag cctgatgcgc agacatcgcc gccgcctgtt tgcgcagccg
     1621 ccgcgccttc ttgcggagca gccacgacat ggtcaggcgt tcggtggtgg ccttgatcga
     1681 cgacagaaag ccgaacagcg ccagcagcgc gccgaacacc cacagcgaga gattgaatgc
     1741 accgaccgcc agcagcaagg cgccgcggcc gagcagcttc aggatcgcgc gggtctggcc
     1801 gcccttggat tccgccagcc gtgcggcgcg agcgacatcc ttcgggccct cggcgatttt
     1861 caacgtctcg agcgccccgc gggtgccggc cttctcactg acgcggccga cgtccttggc
     1921 aagacggacg agcccgccgg ccttctcggc gcgaaacgcc gccctgatcg ctgtcatggt
     1981 ctcccccggc cgcatcaccg agccggatgc gaccgcatgt tgcagcatcg gcgcgtcaac
     2041 cacctcgcgt gcggaacggc tggcccatcg cgtgagcccc tcgcccagcc gcccgacctt
     2101 gcgggcatcc ttgaccagcg tgagaccggc gtgcaccggc accgcgccac cgaccgagat
     2161 ataggtcacc gccgttaccg caaggcccgc cgtggccagg cccagtatca gccgatcggt
     2221 gtcctcgccc atcgccagat gcttgccttc gcgcaccacg tccctgatgt cgccgaacac
     2281 gaacaggtcg ccggcgaccg tgcccgacag gctggcgaca tcatcggcgt ttccggtcac
     2341 cagcccggtg gcgaagcgtt ttgcaaaatg tgacgacgaa ttttcttccg tgacggcctc
     2401 ggatacacgt cgcgacagct catcggcaac aggaatgccc ttttcccggg cgagttcgac
     2461 aaagctgctg gcaagatcgg catcttttgc ggcaagtgcc gcctcgatat tcgctgcgat
     2521 gaccggcttg ttgtcccgca gagcggaatt gagctggatg tcggagagcg cagcgggatc
     2581 gtcctgtgcc gccagaatgg cgccggcgtc gcgcgcatgc ggccacagca acgcgcacgc
     2641 caccgcgcac accgtggtac caaccagagc cgatccgatc cgcgcccgct tcatcccaaa
     2701 aacccgatgt tatcgcctcc cttgagatgg tgtgccgctt ttaagacaca acggccacga
     2761 cgaaagaagg gcttttctgg gacgacaata ttgtgtcgaa tttgcatggg cgaatgagcg
     2821 atcggggctc cctgtggtag gctagttcct tccatgagcg ccagtcgcgt tcgccacaga
     2881 gaattctgaa ccacgcccac accagaatca gaaatctggt ctcctttcga atccgctagt
     2941 tcgctcgaaa agcgtgctgc aggggtgcag caactgcgga ccagcgacac taggaatcaa
     3001 ttgcacccgc cagtatggtg cgctgcgttt cgaccccccg cgcctgcctc catcgctatt
     3061 cacggtgaat ccaatgtccg atcatatcgt tccacatttt cacaacgatg ccggcgtttc
     3121 ggtcatcgag atcggctcgc aggaattcat gtgtgtgggc gccaatccgc cgttcgacca
     3181 cccgcatgtt ttcctcgatc ttggcaacga caacgagatc atctgcccct attgctccac
     3241 cctgtatcgt tacgccgccg acctcaaggc cggcgaggcc cggccgcccg aatgcgtgct
     3301 gaaggataaa gtcgcctgag cggccggtgg ccggctcacg caccatcgtc gtcgctggtg
     3361 ccgggatcgg aggattgacg gcggcgctgg cgctggcggc acgaggcttt cgcgtcgtcg
     3421 tt
//
LOCUS       DATNOI010000654         2931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_856700,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000654 DATNOI010000000
VERSION     DATNOI010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856700"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VN968_26355"
     CDS             complement(<1..430)
                     /locus_tag="VN968_26355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32789.1"
                     /translation="MSKSRTSLACALALSWAVPAFAQAPEPISIVVFGPPSLGAFLPP
                     VIRAQKLDEKNGLAIKFEERTPDAYTAQFNSGEFKLGGSASLLTVGLADTRGVKVTYL
                     FNLFDYWGAVVTSRPEIKTLRDLEGKEVAAAKGTTNWVMFD"
     gene            complement(460..927)
                     /locus_tag="VN968_26360"
     CDS             complement(460..927)
                     /locus_tag="VN968_26360"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HXL32790.1"
                     /translation="MKLDIGGVETIQAPVDVLWKALNDPTVLTRCIPGCKTMTEIAPD
                     AYKVEMQLRVAAVGGSFEGEIALSDKEPPKACSIKVSGAGTLGHGNGSARFQIEPDGP
                     TVSRLTFQGAGEIGGLVAGVGQRILSSVSKHLVGRFFTALRKEFETPVEGAAE"
     gene            complement(938..1915)
                     /locus_tag="VN968_26365"
     CDS             complement(938..1915)
                     /locus_tag="VN968_26365"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL32791.1"
                     /translation="MIIDCHAHLVPPSLLDAIRTQASHFPSIRLIEDGASLGFSFAGG
                     KPTRPVSKPLSDLPARLKWMDEQQIERQVVGGWLDMFAYEVPAEEGVNWSRLINTHLA
                     QTAMREPRFVPLATVPLQDGARAAEVLREAHAAGFKGTMIGTQPKGKGGVLDDPSLKP
                     FWEAANELGSVVFIHPVFESGDDRVHDYGMANAVGRITDTLIAMSRLIYAGHVTRFAN
                     AKIVAGIGGAALPYVIGRLRRNYSLDKDKLGDPDAALAAMHYDTIVQDPRALRYLADI
                     VGAERIMMGSDMPFPIGDLAPLKIVMETKFSDAERAAINGGLAQRLFGL"
     gene            complement(1912..>2931)
                     /locus_tag="VN968_26370"
     CDS             complement(1912..>2931)
                     /locus_tag="VN968_26370"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL32792.1"
                     /translation="FEPLLNEKNTTTTWMDSCRIVIDGLGFVTVTIHNTSSGQGHETL
                     VATVIGEVLQIEPDLIRVIRPDSLACLPSNSPVGSRMAIMLGGAAFHAAQKLKAKLMR
                     IGAHQFGLGADKVVYAGGGVAVPNGDKQLGWAELVNIAHRNYHLLPEGMEPGLESTHV
                     MQVPTGGSLPKDGRVQMYPCHSFEFHVVLMAIDPALGKPEIRRYVIGHDCGTVINPHI
                     VKGMTLGGVAHGLGAALLEEFVYDDEGHLITQSFMDYLLPSSHEVPEVEIVHHCTPSP
                     YTVFGQKGSGESGYLGAPAAISAAINDCVAPLGISFNKLPIRISAIGDAIADAQQGKP
                     QEQAK"
BASE COUNT          544 a          934 c          914 g          539 t
ORIGIN      
        1 agtcgaacat cacccagttg gtggtgccct ttgccgcggc gacttccttg ccttcgagat
       61 cccgcagggt cttgatctcg ggccgggacg tgaccaccgc tccccagtaa tcgaacaggt
      121 tgaagagata agtcactttg acgccgcgcg tgtcggcaag gccgaccgtc agcaaggacg
      181 cgctaccgcc aagcttgaat tcgccggagt tgaattgcgc ggtataggcg tcgggggtgc
      241 gttcctcgaa tttgatcgca agaccatttt tctcgtcgag cttttgcgct ctgatcaccg
      301 ggggcaaaaa cgcgccgagc gacggaggcc cgaatacgac gatcgaaatc ggttccggcg
      361 cctgagcgaa tgcaggcaca gcccacgaca gcgccagggc gcaggcaagg ctggtgcgac
      421 ttttactcat ggccgtcctc ccaaaatctc tcgttaaagc tattccgcag caccctcgac
      481 cggcgtctcg aattccttgc gcagcgccgt aaaaaaccgt ccgacaagat gcttggagac
      541 gctgctcaaa atcctctggc cgacgcccgc gaccagcccg ccgatttcac cggcgccctg
      601 aaatgtcagg cgcgacacgg tcggcccgtc cggttcgatc tggaagcgcg cgctgccatt
      661 gccgtggccg agcgtgccgg cgccggagac cttgatgctg caggccttcg gcggctcctt
      721 atccgacagc gcgatctcgc cctcgaacga gccgcccacc gccgcgacgc gcagctgcat
      781 ctcgaccttg taggcgtcgg gcgcgatctc ggtcatggtc ttgcagcccg ggatgcagcg
      841 cgtcaacacc gtgggatcgt tgagcgcttt ccagagcacg tcaaccggag cctgaatggt
      901 ttcgacaccg ccgatatcga gtttcatgga tacgtcctca cagcccgaac agccgctgcg
      961 ccaaaccgcc attgatggcg gcgcgctccg cgtccgagaa tttggtctcc atgacaatct
     1021 tcagcggcgc gagatcgccg atcgggaacg gcatatccga acccatcatg atcctttcgg
     1081 cgccgacgat gtcggcgagg tagcgcagcg cgcgggggtc ctggacaatg gtgtcgtaat
     1141 gcatcgcggc aagggccgcg tcgggatcgc cgagcttgtc cttgtcgagc gaataattcc
     1201 ggcgcaggcg gccgatcaca taaggcagcg ccgcgccgcc gataccggcg acgatcttgg
     1261 cgttggcaaa gcgggtgaca tggccggcat agatcagccg cgacatcgcg atcagcgtat
     1321 cggtgatccg ccccaccgca ttcgccatcc cgtaatcgtg gacgcggtca tcgccgcttt
     1381 cgaacaccgg atggatgaaa accaccgatc cgagttcgtt ggcggcctcc cagaatggct
     1441 taagcgaggg atcgtcgagc acgccgccct tgcccttggg ctgggtcccg atcatcgtgc
     1501 ccttgaaacc ggcggcgtgg gcttcgcgca atacctcggc ggcacgcgcg ccgtcctgca
     1561 agggcacggt ggccagcggc acaaaccgtg gctcgcgcat cgcggtttgc gccaggtggg
     1621 tgttgatcag ccgcgaccag ttcacgccct cctccgccgg cacttcatag gcaaacatgt
     1681 cgagccagcc gccaaccacc tgacgctcga tctgctgttc gtccatccat ttcaggcggg
     1741 cgggcagatc gctcagcggc tttgaaaccg gccgcgtcgg cttgccgccg gcaaacgaga
     1801 aaccgaggct tgcgccgtct tcgatcaggc ggatcgaggg aaagtgagag gcctgggtgc
     1861 ggatcgcgtc cagcagactt ggcggcacca gatgggcgtg gcagtcgatg atcatttggc
     1921 ttgctcttga ggtttgcctt gttgcgcatc cgcgatggca tcgccaatgg cggagatacg
     1981 gatcggaagc ttgttgaagg aaatgcccag cggcgcgacg cagtcgttga tcgcggctga
     2041 tatcgctgcg ggagcgccga ggtagccgct ttcgccggag cccttctgcc cgaacacggt
     2101 gtagggcgac ggcgtacagt gatgcacgat ctcgacctcg ggcacctcgt gagacgatgg
     2161 caatagatag tccatgaaac tctgggtgat cagatggcct tcgtcatcgt agacgaactc
     2221 ctccagcagc gcggcgccaa ggccgtgcgc gacgccaccc agcgtcatgc ccttgacgat
     2281 atgcgggttg atgaccgtgc cgcaatcgtg gccgatgacg tagcgcctga tttccggctt
     2341 gccgagcgcc ggatcgatcg ccatcagcac cacatgaaac tcgaaggagt ggcacggata
     2401 catctggacc cggccgtcct ttggcaagct gccgccggtc ggcacctgca tcacatgcgt
     2461 gctctcgagg cccggctcca tgccctcggg caacagatga taattgcgat gggcgatgtt
     2521 gacgagttcg gcccagccaa gctgtttgtc gccgtttggg accgcgacgc cgccgcccgc
     2581 gtagacaacc ttgtccgcgc cgaggccaaa ctgatgcgcg ccgatccgca tcagcttcgc
     2641 cttcagcttt tgcgcggcat gaaacgccgc cccgccgagc atgatcgcca tccggctgcc
     2701 gaccggcgaa ttggaaggca ggcacgccag cgaatccgga cggatcacgc ggatcagatc
     2761 cggctcgatc tgcagcacct cgccgatcac ggtggcaacg agagtctcgt gcccctgccc
     2821 cgacgacgta ttgtggatgg tgacggtgac gaagccgagc ccgtcgatga caatgcggca
     2881 ggaatccatc caggtggtgg tggtgttctt ttcgttgagc agcggctcga a
//
LOCUS       DATNOI010000655         5996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_44316,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000655 DATNOI010000000
VERSION     DATNOI010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44316"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..528
                     /locus_tag="VN968_26375"
     CDS             208..528
                     /locus_tag="VN968_26375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL32793.1"
                     /translation="MRIWTIWLCAAATLSGLFAARVDAQAQNFPSRSITLVIPFAPGG
                     STSIVGRVIADKMSELLGEKVVVDNRPGAGGTVGTKAVAKSDPDGYTSAWLHRHARDR
                     TVAL"
     gene            470..1186
                     /locus_tag="VN968_26380"
     CDS             470..1186
                     /locus_tag="VN968_26380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL32794.1"
                     /translation="MATLLLGYTGTLAIGPSLYKNAGYDPRKDFAPIGLIGNAPNSLV
                     VHPSFPAKTVAELIAYAKANPGKVNFGSAGAGTASHITGEYFARSAGITLVHIPYKGT
                     GPALTDLLGGHIPMAFAPIPASHANVSGGMLRALAVTSTTRSNLLPDVPTMAEAGLSG
                     FDASLYYGLVAPAGTPRPIIDKLNKQLRAALASSEVKKQLGLDGTEITPGTPEDYAAF
                     IDKDEKKWSQLVKASGVEPE"
     gene            1234..2436
                     /locus_tag="VN968_26385"
     CDS             1234..2436
                     /locus_tag="VN968_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32795.1"
                     /translation="MLPQAIRRPGYFALALLLIAPQAAALEAAPLPSTTAGAMAQAAS
                     PQAIAEYRRKLKEYLEARAAFDQEADAYWNSIAEKRRGRNAKRRERQTITLDDYVLTQ
                     PPVYAGPRRPVNPQPETVEPREPRERKRIPIVADLLKAAAEHFQFAPQRPAGELEFKR
                     AYARTASAAGLTREQAVRVYSFETGGNGNHDMQSGLSASRPGSRAISTAVGYNQLLTT
                     NSVELMAEQGPEFVRALTAKAALLSGSQRQAMDRKIAVLKRMVALARSVPDEWAAHEK
                     IGNTPQGWAIHAMVLDIDVGPLLQTHKLLTSVIFARARGFARPLTAAELEMMNLTGDG
                     TGLDMVTMPQPMREQVPTSNFFQRGGYERNPVAIRNNTVAKLLAVTDSRMDSNSTLSG
                     AKELAAAF"
     gene            complement(2648..2953)
                     /locus_tag="VN968_26390"
     CDS             complement(2648..2953)
                     /locus_tag="VN968_26390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32796.1"
                     /translation="MTRFSTKTLLIAVGLTALTTSAFAQAPAPTPWVLTPDMGYGYDK
                     EGKTYSYKMGTNNAGLLLKGAKKVPKGTLFFIGQNGQLYMRPGPYLEGDGRFMFGPR"
     gene            3174..3641
                     /locus_tag="VN968_26395"
     CDS             3174..3641
                     /locus_tag="VN968_26395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32797.1"
                     /translation="MKICLGLALSGMTLVPLAQADEFKLSNNQRISCSRGLSPGKLNT
                     ATCKSYAYLFNVKTSEYFRCQISLALTRDNKEVINVQTDGGCTKRPRIFDTDSSYQFD
                     ATETEPPNTNSFFGPGGYSVWAADTAAQKLRGCITISSGLGSDISKCLDMTFQ"
     gene            3873..4676
                     /locus_tag="VN968_26400"
     CDS             3873..4676
                     /locus_tag="VN968_26400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL32798.1"
                     /translation="MDRLKGKIALVVGAGSIGPGWGNGKATAVTFAREGAQVFCVDRN
                     GAAAEETAEIIASEGGKAAAFTSDVSRAGDVEAMVAACLKTYGRIDVLDNNVGIAETG
                     NVVEVSEADWDRVFAVNLKSAFLAMKHVIPVMARQGGGSIVNISSIASIRHLGISYVS
                     YATTKAAMNQMTRATAVEFAAKHIRVNAILPGLMKTPMVEHSAGLAASYAAGNVEEMW
                     RARDAQVPMGHMGEAWDVANAALFLASDESKYITGIELVVDGGVTLKVS"
     gene            4935..>5996
                     /locus_tag="VN968_26405"
     CDS             4935..>5996
                     /locus_tag="VN968_26405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXL32799.1"
                     /translation="MSIQDNSRYQQVYARSLADSEGFWSEAAREIDWIEPAKKIFDPA
                     SGPYGRWFSGAVVNTCYNALDRHVAGGRAGQVALIHDSPLTNKIAKFTYAELLKEVQA
                     LAAIMADFGVAKGDRVILYMPMVPEAVVAMLACARIGAVHSVVFGGFAAKELATRIED
                     AKPKLIFSASCGLEPGRIVQYKPLLDEAIRLASAKPEACIILQRPQQSCELTAGRDHD
                     WARLRSQAIAAKKSADCVPVLATDPLYILYTSGTTGIPKGVVRDNGGHLVALKWSMFN
                     LYGVKPGEIWWCGSDIGWVVGHSYIVYGPLIHGATSIMYEGKPVGTPDAGAFWRLISE
                     HKAVALFTAPTAFRAIKKED"
BASE COUNT         1129 a         1929 c         1878 g         1060 t
ORIGIN      
        1 atcggaatat tcctttcagt gtcattccgg ggcgcgcgca gcgcgaaccc ggaatctcga
       61 gattccccga tgtgcaaatg cacatctgag gtctggtcct tcggaccatc ccggaatgac
      121 gatccctgga tcgggttgcg aacgtcggtc ctttcgacga cgatgcggcc aaacaacaac
      181 gatgatcccc cgggaggctc tgcaaaaatg cgtatctgga cgatctggct ttgtgccgcc
      241 gcaacgctgt ccggcttgtt cgccgcgcgg gttgacgcgc aagcccaaaa ctttccgagc
      301 cggagcatca cgctcgtcat tccgttcgcg cccggcggct caacctcgat cgtcggccgc
      361 gtgatcgccg acaagatgag cgaattgctt ggcgaaaaag tcgtggtcga taaccggccc
      421 ggcgccggcg gcaccgtcgg caccaaggcg gtcgcgaaaa gcgatcccga tggctacact
      481 tctgcttggc tacaccggca cgctcgcgat cggaccgtcg ctctataaaa atgccggcta
      541 cgatccgcgc aaggattttg cgccgatcgg cctgatcggc aacgcgccga attccctggt
      601 ggtgcatcct tcgttccccg ccaaaaccgt ggccgagctg atcgcctatg ccaaggccaa
      661 tcccggcaag gtcaatttcg gctctgccgg cgccggcacg gccagccaca tcaccggcga
      721 atattttgca cgttcggccg gcatcacgct ggtgcacatt ccctacaaag gcaccggccc
      781 ggcgctgacc gatctgttgg gcgggcacat cccgatggca ttcgcgccga tcccggcttc
      841 gcacgccaac gtctcgggcg ggatgttgcg cgcgttggcg gtaaccagca cgacccgctc
      901 caacctgctg ccggatgtgc cgacgatggc cgaagccggc ctgtccggct tcgacgcctc
      961 gctttattac ggactggtgg cgccggccgg cacgccgcgc cccatcatcg acaagctcaa
     1021 caagcaattg cgggcggcgc tcgcctccag cgaggtgaag aagcagctcg ggcttgacgg
     1081 taccgagatc acgccgggca cgccggaaga ctatgccgcc ttcatcgaca aggatgagaa
     1141 gaaatggtcg cagctcgtca aggccagcgg cgtcgagccg gagtgaacga tttcgggctg
     1201 acttgcgccg cccgcgcttg ctaaaacggt cgcatgcttc ctcaagcgat tcgccgtccc
     1261 ggatatttcg cgctggcgct gctgctgatc gcgccgcagg cggcggctct cgaggccgcg
     1321 ccgcttccgt caacgacggc cggcgcgatg gcgcaggccg cttcgccgca ggcgatcgcg
     1381 gaataccgcc gcaagctgaa ggaatatctc gaggcgcgcg cggcgttcga ccaggaggcc
     1441 gacgcctact ggaattcgat cgccgagaag cggcgcggcc gcaacgccaa gcggcgcgaa
     1501 cgccagacca tcacgctcga cgattatgtg ctgacacagc cgccggtcta tgccgggccg
     1561 cggcggccgg tgaacccgca gcccgagacc gtggagccac gcgaaccccg ggagcgcaag
     1621 cgcattccaa ttgtcgccga tctcctcaag gccgccgcgg agcatttcca gtttgccccg
     1681 cagcggcccg cgggcgaact cgagttcaag cgcgcctatg cccgcacagc atcggcggcc
     1741 gggctgacgc gcgagcaggc ggtgcgggtc tattcgttcg agaccggcgg caacggcaat
     1801 cacgacatgc aatcgggttt gagcgcgtcg cggccgggct cgcgcgcgat ctcgaccgcg
     1861 gtcggctaca accagctgtt gaccaccaac agcgtcgagc tgatggccga gcagggcccg
     1921 gaattcgttc gtgcgttgac ggcaaaggcc gcgcttttgt ccggcagcca gcgccaggcg
     1981 atggatcgca agatcgcggt gctgaagcgg atggtggcgc tggcgcgctc ggtgccggac
     2041 gaatgggcgg cgcacgagaa gatcggcaat acgccgcagg gctgggcgat ccatgccatg
     2101 gttctggata tcgatgtcgg cccgttattg cagacccaca agctgctcac ttccgtgatc
     2161 ttcgcccgcg ccaggggttt tgcgcgcccg ctcaccgccg ccgaactcga gatgatgaac
     2221 ctgaccggcg atggcaccgg gctcgacatg gtgacgatgc cgcagccgat gcgcgagcag
     2281 gtcccgacct cgaatttctt ccagcgcgga ggctacgagc gcaatccggt ggcgatccgc
     2341 aacaacaccg tggcgaaact gctggcggtc accgacagca ggatggactc caacagcact
     2401 ctctccggcg ccaaggaact ggcggcggcg ttttagtatt caccctcccc tggaggggga
     2461 gggtcggctc accattgagc gcagcgaaat gtgagacggg gtggggtgat ctctcaactc
     2521 gggcaccgtt cggaaacgaa agaccgtcac ctcaccccgc cgctcatttc attcgagtcg
     2581 acccagagag cgagcttcgc tcgtctcgac ccctccaggg gagggtatag ggcgcgacga
     2641 ttgtccctca ccgcgggccg aacatgaacc tgccgtcgcc ctcgaggtag gggccgggac
     2701 gcatgtagag ctgtccgttc tggccgatga agaacaacgt gcccttcggc accttcttcg
     2761 cgcccttcaa gagcaggccg gcattgttgg tgcccatctt gtaggaatag gtcttgccct
     2821 ccttgtcgta gccgtaaccc atatccggcg tcagcaccca gggcgtcggc gcgggcgctt
     2881 gcgcgaacgc ggacgtcgtc agcgcggtaa gcccgactgc aatcaatagg gtctttgtcg
     2941 aaaatctcgt catcctcagt tctcccggca cccgatcgga acgtcgatcc catcttgaac
     3001 aaatcacgaa cgccatcgcc gcgtcaatat cgcgagtcta ttcgccgcag cgcgcctcca
     3061 taagacttta gactttgtga ttccccgcat cgttcttcgc gactatgttc cccttccggt
     3121 ctagaaccgg ggttgggaaa cacgtcatag gggcgattcg gatgaaccac gtcatgaaaa
     3181 tttgtttggg ccttgctttg tcgggtatga cgctggttcc gctcgcccag gccgatgaat
     3241 tcaagctctc caacaatcag cggatttcct gcagccgggg cttgagtccg ggaaaactca
     3301 ataccgcgac ctgcaaatcc tacgcctatc tcttcaacgt caagacgtca gaatatttca
     3361 ggtgccagat ctcgctggcg ctgacgcgag acaacaagga agtgatcaac gtccagaccg
     3421 acggcggctg caccaagaga ccccgcatct tcgataccga ttccagttac cagtttgacg
     3481 ccaccgaaac cgagccgccc aacaccaatt cgtttttcgg tcccggcggc tattcggtat
     3541 gggcggccga cacggcagcg cagaaactgc gcggctgcat caccatctcc tccgggctcg
     3601 gctccgacat ctcgaagtgc ctggacatga cctttcagtg agggatcccg ctgctcctga
     3661 tggtgaggag gcgcgctccc ggcagcgcac ttgcgctgtc cggtgcgccg tctcgaacca
     3721 tcaggccccg atgctactca tcgttcgaga cgcggccaac gggtccgcgc gaagcgcggc
     3781 ccgacgataa actccgacgc tgctcttcag gatgagggca ggatgatgcg tcattcgggc
     3841 cacgattgcc gaaatcatac agggagacgt cgatggatcg gctcaaggga aagatcgcgc
     3901 tggtggtcgg cgctggctcg atcgggcccg gctggggcaa tggcaaggcg accgcggtga
     3961 cctttgcgcg cgagggcgcg caggtgtttt gcgtcgatcg caacggcgcc gccgcggagg
     4021 aaaccgctga gatcatcgcc tcggaaggcg gcaaggcggc cgcgttcaca tcggatgttt
     4081 cccgcgccgg cgatgtcgag gcgatggtcg cggcatgcct caagacctat ggccgcatcg
     4141 acgtgctcga caacaatgtc ggcattgccg agacgggcaa cgtggtcgaa gtgtcggagg
     4201 ccgattggga ccgcgtgttc gcggtcaatc tcaagagcgc gttcctcgcc atgaagcacg
     4261 tcatcccggt gatggcgcgg cagggcggcg gctcgatcgt caatatctcc tcgatcgcct
     4321 cgatccgtca tctcggcatt tcctatgtca gctatgccac gaccaaggcc gcgatgaacc
     4381 agatgacgcg cgccaccgcg gtcgagttcg cggccaaaca tatccgcgtc aacgccatcc
     4441 tgccgggcct gatgaaaacg ccgatggtcg agcattccgc cggccttgcg gcgagctatg
     4501 ctgcgggcaa cgtcgaagag atgtggcgcg ctcgcgacgc gcaggtgccg atggggcaca
     4561 tgggcgaagc ctgggatgtg gccaacgcgg cgctgtttct cgcctccgac gaatcgaaat
     4621 acatcaccgg catcgagctg gtggtcgacg gcggcgtcac gctgaaggtg agttagctcg
     4681 attttcaacc gccgtcattc cgggacacgc ggcttcgcgt ggacccggaa tccatactcc
     4741 cgatggtggt tatggattcc gggctcgctc gctttgctcg cgccccggaa tgacagtaat
     4801 caagaacagg ccaattgcgc ccttgaaacg atgccgcttc ctgtgacaat gcgagatcat
     4861 cgccgcgtca ctatggccgg gcccgtcccg gccgtggatg acgggaacga ggtgacggag
     4921 cgggagcgag accaatgagc atccaggaca acagccgcta tcaacaggtc tatgcccgct
     4981 cgctggccga ttccgaaggg ttttggagcg aggcggcgcg cgagatcgac tggatcgagc
     5041 ccgcgaagaa aatcttcgat ccggcaagcg gtccctacgg ccgctggttt tccggcgccg
     5101 tggtcaacac ctgctacaat gcgctcgacc gccatgtcgc gggcggacgc gccggccagg
     5161 tggcgctgat ccacgactcg ccgctcacca acaagatcgc gaaattcacc tatgccgaac
     5221 tcttgaaaga ggtgcaggcg ctcgccgcca tcatggccga tttcggggtg gcaaaaggcg
     5281 accgcgtcat cctctatatg ccgatggtgc cggaagccgt ggtcgcgatg ctggcctgcg
     5341 cgcggatcgg cgcggtgcat tcggtggtgt tcggcgggtt cgcggcgaag gagctggcga
     5401 cccggatcga agatgcaaaa ccaaaactga tcttttcggc gagctgcggg ctcgagcccg
     5461 gccgcatcgt gcaatacaag ccgctgctcg acgaggcgat caggctcgcc agcgccaagc
     5521 ccgaggcctg catcatcctg cagcggccgc agcaatcctg cgagctcacc gcggggcgcg
     5581 atcacgactg ggcgagattg cgcagccagg ccatcgcggc gaaaaaatcc gccgattgcg
     5641 tgcccgtgct tgccaccgat ccgctctaca tcctctacac ctcgggcacc accggcatac
     5701 ccaaaggtgt ggtgcgcgac aatggcgggc atctggtggc gctgaaatgg tcgatgttca
     5761 atctctacgg cgtcaagccc ggcgagatct ggtggtgcgg ctccgacatc ggctgggtgg
     5821 tcggccacag ctacatcgtc tacgggccgc tgatccatgg cgcgacctcg atcatgtatg
     5881 agggcaagcc ggtcggcacg cccgacgccg gcgcgttctg gcggctgatc tccgaacaca
     5941 aggcggtggc gctgttcacc gcgccgaccg cattccgcgc catcaagaag gaagac
//
LOCUS       DATNOI010000656        12787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2204937,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000656 DATNOI010000000
VERSION     DATNOI010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12787
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2204937"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="VN968_26410"
     CDS             complement(<1..1515)
                     /locus_tag="VN968_26410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HXL32800.1"
                     /translation="MTFSSSFGQDRAPGSGVTAVLGPTNTGKTHLAIERMLAHSSGVI
                     GLPLRLLAREVYNKIADRAGVDSVALITGEEKIKPPRPRFWVSTVEAMPRDLDVSFLA
                     VDEIQIAADLERGHVFTDRILHRRGRDETLLLGAATMRPIIERLLPGASIVTRPRLSQ
                     LEFAGDRKITRQPRRTAIVAFSADEVYAIAELIRRQHGGAAVVLGSLSPRTRNAQVAM
                     FQNGDVDYLVATDAVGMGLNLDVDHVAFASDRKYDGYQFRRLNPSEFAQIAGRAGRAT
                     RDGTFGTTGRCAPFEPELVNALQNHTFDSVKVLQWRNSKLDFSSLGALQVSLALTPSH
                     EALTRAPIAEDLRVLDHAARDADVREMAHGAAAVERLWDACQIPDYRKIAPAAHAELV
                     TTLFEFLMKKGRIPDAWFSAQVEQADRTDGDIDTLSGRIAQIRTWTFVANRPDWLADP
                     EHWQGIAREVENKLSDALHERLTERFVDRRTSVLMRRLRENTVLSTEIGKTGEVT"
     gene            complement(2066..2971)
                     /locus_tag="VN968_26415"
     CDS             complement(2066..2971)
                     /locus_tag="VN968_26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HXL32801.1"
                     /translation="MTVHRRDLKDRLPVTTHRTISLSAALGLRVSASLCVGALLLLAG
                     LPPAFAQGRLDARYEATLSGIPVGTGAWTIDIADDQFSASASGGTAGLLKAFAAGSGT
                     GAAQGRVVNGLPVATNYSASTTTSKKTEAIHMVLSNGNVREYGIEPAPPVDPDRLPIT
                     EAHRRGVFDPMTGSMLRAPASVDPLSPEVCRAGAPIFDGRMRYDLKLDFKRMEMVRAE
                     RGYHGPALVCAVYFSPVAGYIPDRPVIKYLAAQRNIEIAFVPVAGTRILVPFWMRIPT
                     PLGPATLEATQFITQATPPRVAKTQ"
     gene            3269..3577
                     /gene="rpmB"
                     /locus_tag="VN968_26420"
     CDS             3269..3577
                     /gene="rpmB"
                     /locus_tag="VN968_26420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HXL32802.1"
                     /translation="MSRRCELTAKGPQVGHKVSHSNIKTKRRFLPNLCNVTFISDALG
                     RNVRLRVSTNAIKSVDHRGGLDAFLLKAKATELSPRALELKRAIEKKKAAGAPEQKAG
                     "
     gene            complement(3620..4603)
                     /locus_tag="VN968_26425"
     CDS             complement(3620..4603)
                     /locus_tag="VN968_26425"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32803.1"
                     /translation="MRRREFFAIVGGSLLWPRAARAQQAGKTYRVGLLATGGVVAAMD
                     ERRNSILEGLAANGFFEGRNLEFDVRSGEGRSERLAEFAAALQAANVDAIVTFGYPAA
                     AAAKTSATDIPVVVTGAGDPVATGLADSLARPGGHVTGMTELSTELSGKRLEILKEAV
                     PNLRRVAMLFNATDLGMTLRSQAAANAARELNVDVQTLGVREPNDFDEAFAEMTKARP
                     DAMLMVSDALTVLNRKRIMNFAAANRLPAIYEFSALVRDGGLMSYGPNQRAIGVRAGD
                     FVARILRGARPADLPLEQPTQFELAINLKTAKALGLELSPILIARADEVIE"
     gene            complement(4711..8226)
                     /locus_tag="VN968_26430"
     CDS             complement(4711..8226)
                     /locus_tag="VN968_26430"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXL32804.1"
                     /translation="MIYAFKEYLLDADRRELRRGAALIAAEPQTFDLLLYLIRNRERV
                     VSKDDLMAAVWSGRIVSESTLSSQINAVRHAIGDDGERQILLRTLPRKGFRFVGEVRE
                     QCEREKLRLAEVASAARNDAPEAARSRSDRPERRQLSVMICSLAGAGAHSLARDPEDL
                     LAVMSALQDRIKTVITEHDGFVGQILGGEVVVYFGYPRAREDDAERAVHAGLAVIKAV
                     ASLNTRSDKPLQARVAVATGLVVVGDLNELGIAADHAAIGDALPLAAGLAALAEPGAL
                     VISATTRRLIGALFDTRGLGSLKLKSSADPVEAWQVLSEGAATSRFEALHSKPTKLIG
                     REEELALLQRRWSQIKSGEGRLVLVYGEPGIGKSRLVFALQETIKEEPHHCLRLQCSP
                     HRSQTAFYPVINQLEHAAGLAAGDSDAAKLDKLAALLAPSSQNPARDTALFAELLSIP
                     CQGSSVLSISPQRRKELLLEQLVGQIEGLAGEKPVLIVLEDAHWVDPTTQELFDILAE
                     RIRTLPVLLIITYRPEFVPPWLGQSHVTVLTLNRLGRRDNIEVIRQVANGRDFPPVLL
                     EQIVARTDGVPLFIEEMTKSVLESDILREQNGAYVLAEALPPLAVPTTLQASLVARLD
                     RLASLRAVVQAGSALGREFAYTSIRAVFDLGDAELAPLLRQLVASELVHQRGVVPHAV
                     YTFKHALVQDAAYGTMLKSQRVQIHVRIVETFERQFPEMLERNPDVLAYHCSEAGAFG
                     KAIDYWLKSTRLSLVRSAGIEAQAQLEKAVTLLPEITDPERRRQFEARIHVELGNTYV
                     MTKGFASPDVATALTRARDLLDEAAHPFEALRALGGLCNYHLIRSEAPKVLQLAEPFL
                     RRRLDPPSAMIGHYEAGAACLHIGRFEDAKFHLETGLSLYEEESCRPVAFMAGTHVRA
                     FSLVWLSLAYLYLGRLKLATETREAAVSDARSRLHPFTLVSVLLASARFFLHTRNLPA
                     ATATTEEGFAIATEQRSPYHISRANILKAVNLVEGGRAVEGIALMDAALIEHRNTGAN
                     FQSSFNLSCLADAYARAGNSKRALDFAGQAISEIERTGERWWAAEAQRIKGNILLAAN
                     PADRRRAEDCFLLALKSAQSQNARFWQLRAAHSLASLWSGQGRDAEARKLLAPIYDEF
                     TDGIDLPDLDDARGLLDRLGRPVSG"
     gene            8363..8845
                     /locus_tag="VN968_26435"
     CDS             8363..8845
                     /locus_tag="VN968_26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6647 family protein"
                     /protein_id="HXL32805.1"
                     /translation="MDALLTAIVIWISANYALPACLDHPRIERVASIEMAGLRTKGLL
                     SALARQASVIEVQESSFEKRRDVVALYNDQTRTIYLSDKWTGTTPSELSVLVHEMVHH
                     LQNEAGTAYECPAEREKLAYEAQDRWLRLFGRNLEAEFEVNGLALLVSTSCGMAMGLH
                     "
     gene            complement(9176..10402)
                     /locus_tag="VN968_26440"
     CDS             complement(9176..10402)
                     /locus_tag="VN968_26440"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXL32806.1"
                     /translation="MAVASAAMIPAAPLTGIAAPLNKLAHSEEIDRALQASVSAREIP
                     GVVAMAAKEQSVVYEGAFGFRDIAAASRMSTDTVFRIASMVKLLTSVAALQLVERGTL
                     KLDEPAGNIDPALASAQILTGFDAKGVPQLRPAQKPLTLRNLLTHTSGFSYPLWDPNV
                     GRYLKRARKNPHLPRMPLMFEPGSRWAYGGSLDRVGRMVEIAGGQNLDRYFSDHITGP
                     LGMNDTGFSLGEQQRAREASLHVRDGTGKLVPQPLEKPKVPQAFSGGGGIYSTAPDYL
                     TLLQALLNGGSFRGTSILQPETVASMSTNQIGNVEAGILRTTNPALSNDVDFFPGVRL
                     RWGLGHMINIDPVPDGRRAGSLTWAGLFNTYYWIDPTMRIAGVIMMQILPFADRQALR
                     VYRQFERGICRALGSA"
     gene            complement(10419..11276)
                     /locus_tag="VN968_26445"
     CDS             complement(10419..11276)
                     /locus_tag="VN968_26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HXL32807.1"
                     /translation="MVSWTVVGFDSAWTDNPKALGAVCAIRCDHHGHLSSSNPQLASF
                     DQAFDFIAAEQSRCDVCVVALDQPTIVPNIGGMRPVDKVAASLISWLGGGVQPANRSK
                     KGMFDDDAPIWRFKQRLSASDDAEAARTAEQGLFLIEAFPALALPSLEAAYFGRLVAP
                     RYNPGNGRRFRLPDWTGVTTMVHRHAVTLGLVSLATWARNASLISKPRKSDQDKLDAA
                     ICALVGMLWRSKNRSESIMIGNAELGYMIAPASPEVRARLRTAAAVRNVPIDGAHLQD
                     ANDRADHPT"
     gene            complement(11356..11478)
                     /locus_tag="VN968_26450"
     CDS             complement(11356..11478)
                     /locus_tag="VN968_26450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32808.1"
                     /translation="MSALIQAHGFQTISVKKQTQLSGIAVRYEMDNGHKRYFRA"
     gene            11777..12325
                     /locus_tag="VN968_26455"
     CDS             11777..12325
                     /locus_tag="VN968_26455"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXL32809.1"
                     /translation="MLGLTGCASTPARVAEVDPAYRRTDVAYATREPRGTIVVDPLQH
                     YLYYVEGGGQARRYGIAVGGEGFGWSGVATVHSKQEWPDWYPTREILERHPELTAGLK
                     ELQGGQGMPGGPDSPLGARALYLWQGNKDTLYRIHGTNEPWKIGSNVSSGCIRMINDD
                     AIDLYDRVRINTRVVVLASNVG"
BASE COUNT         2435 a         3984 c         4037 g         2331 t
ORIGIN      
        1 ggtcacttcg ccggtcttgc cgatttccgt actcaaaacg gtgttctccc gcaggcggcg
       61 catcaataca ctggtacggc ggtcaacgaa acgctccgtg agccgttcat gcagcgcatc
      121 ggacaattta ttttcaactt ctcgggcgat tccctgccaa tgctcggggt cggccagcca
      181 gtccgggcgg ttggcgacaa aagtccaggt gcggatttgc gcgatccggc ccgacagcgt
      241 gtcgatatcg ccgtctgtgc ggtcggcctg ctccacctgg gctgaaaacc atgcgtcggg
      301 aatccgacct ttcttcatca aaaattcgaa cagcgtcgtc accagctcgg catgggcggc
      361 cggcgcgatc ttgcggtaat ccggaatctg gcaggcgtcc cacagccgtt ccacagcagc
      421 tgcgccatgc gccatctcgc gcacatcggc gtcgcgcgcg gcgtgatcga gcacccgcag
      481 atcttcggcg atcggggcgc gggtcagcgc ctcatgcgag ggcgtcagcg ccagcgacac
      541 ctgcagcgcg cccagcgagg aaaaatccag ttttgaattg cgccattgca aaaccttcac
      601 actgtcgaag gtgtggttct gcagcgcgtt caccagctcc ggctcgaacg gcgcgcagcg
      661 gccggtggtg ccgaaagtcc catcacgcgt ggcgcgcccg gcgcgcccgg cgatctgcgc
      721 gaattcggac ggattgagcc gccggaactg atagccgtca tatttgcggt cggaggcgaa
      781 ggcgacatga tcgacgtcga ggttgagccc catgccgacc gcgtcggtcg caacaagata
      841 atcgacatcg ccattctgga acatcgccac ctgcgcgttg cgggtgcgcg gcgacagcga
      901 gcccagcacg acagccgcgc cgccatgctg gcggcggatc agttccgcga tcgcgtaaac
      961 ttcgtcggcc gaaaacgcga cgatcgcggt gcgccgcggc tgccgcgtga tcttgcggtc
     1021 tccggcgaat tcgagctgcg acagccgcgg ccgcgtgacg atcgacgcac ccggcagcag
     1081 ccgctcgatg atcggccgca tggtggcggc ccctaacagc agcgtctcgt cgcggccgcg
     1141 ccggtgcagg atgcggtcgg tgaagacgtg gccgcgttcg agatcggcgg cgatctggat
     1201 ttcatcgacc gcgaggaacg atacgtcgag atcccgcggc atcgcctcga cggtggagac
     1261 ccagaaccgc ggcctcggcg gttttatttt ttcctctccg gtgatcagcg cgaccgaatc
     1321 gacgccggcg cgatcggcga tcttgttata gacctcgcgc gccagcagcc gcagcggcag
     1381 cccgatcacg ccagaggaat gcgccagcat ccgctcgatc gcgagatggg tcttgccggt
     1441 gttggtcggc ccgagcacgg cggtgacgcc ggagccggga gcccggtctt ggccgaacga
     1501 agaagagaaa gtcatatttt gcaggtgttt cttttattgc tgcgcctttc gtcatcgcga
     1561 gcgcagcgaa gcaatccatt gggccacgaa ggaagatgga ttgcctcgtc gcttcgcttc
     1621 tcgcaatgac gggggaaact caactcattt gttgtttggc accatttagc gccgatttgt
     1681 ctcacaatgc gacgcgtttg aggtcgcggc ttaagctctg gaacggttgc ggaacgaatc
     1741 gcgcccgaat cggtgactcg cctgccctcc ggcttcgttc accgcaacat ctcgcgtcgg
     1801 cccttgggtt tcggcactag atcaagtctg gcgcggctcc acaagggacc gattggcgct
     1861 gcattcctta aacgttttgc gcatggcgag tcgaatcaga actgacaaag agtcaacagg
     1921 attcggcggc tttcgtcatt ccggggcgat gcgcagcatc gaacccggaa tctcgagatt
     1981 cccccgatgc gcaattgcgc atctgaggtc tggtgcttgc gcaccatccc ggaatgacgg
     2041 gtctcctaag gtcactctgc ttcggtcact gcgtcttggc cacccgcggc ggcgtggcct
     2101 gggtgatgaa ctgggtggcc tccagcgtgg cggggccgag cggggtcggg atcctcatcc
     2161 agaacggcac caggatgcgg gttcccgcca ccggcacaaa ggcgatctcg atgttgcgct
     2221 gggcggcgag gtatttgatc accgggcggt cggggatgta gcccgcgacc ggcgagaaat
     2281 ataccgcgca gaccagcgcc gggccgtgat agccccgctc cgcccgcacc atttccatgc
     2341 gcttgaagtc gagcttgaga tcgtagcgca tgcggccgtc gaagatcggc gcgccagcgc
     2401 ggcagacctc ggggctgagc ggatcgacgc tggcgggcgc gcgcaacatc gatcccgtca
     2461 tcgggtcgaa cacaccgcgg cgatgcgctt ccgtgatcgg aagccggtcc ggatcgaccg
     2521 gcggcgccgg ctcgatgccg tattccctga cattgccgtt cgacagcacc atgtggatgg
     2581 cttcggtttt cttcgacgtc gtggtgctcg cggaataatt ggtcgcgacc ggcaggccgt
     2641 tgacgacgcg gccctgcgcc gcgccggtgc cggagccggc cgcgaacgct ttcagcaggc
     2701 ccgcggtgcc gccggaggct gacgccgaaa actggtcgtc cgcgatatcg atggtccagg
     2761 cgccggtgcc gaccggaatg ccggatagcg tcgcctcata acgcgcgtcc agcctgccct
     2821 gcgcgaaagc cggcggtagc cccgcgagca gcagcaaggc accgacgcag aggctcgccg
     2881 acacccgcaa gcccaaggcc gcggagagcg aaatcgtgcg gtgcgtggtc actggaagcc
     2941 ggtccttgag gtcgcgcctg tgaactgtca tttaggcgaa aatagaggcc aaacaacgga
     3001 ttggtgttgc gcaaatccgc gttatctgcg ggcggaactg ccgcaaggat ggtgtcggcg
     3061 gggaatacgc cgttttaatg atggttgacg gctgcagaac gcccgtaaaa ccgacgtttt
     3121 cagcaccgtc agacacccca aaaccttgac gccccgacgc tctcccccta tacgtccgcc
     3181 gctcccttcg atggacgtga ttttgaaccc ccgggccgcg cgccatgcgc ttttcggaag
     3241 tgccggggat ggcagaggat ttttgaagat gtcgcggcgc tgtgaactga cggccaaggg
     3301 cccgcaggtg ggccacaagg tcagccactc caacatcaag accaagcgcc ggttcctgcc
     3361 gaacctgtgc aacgtcacgt tcatctccga cgcgctcggc cgcaacgtac gcctgcgcgt
     3421 ttcgaccaac gcgatcaaat cagtcgatca ccgcggcggc ctcgatgctt tcttgctgaa
     3481 ggcgaaagcg accgagctgt cgccgcgcgc gctggaactg aagcgggcga tcgagaagaa
     3541 gaaggcggcg ggggcgccgg agcagaaggc gggctgacgc cctcacctct ccccgcaagc
     3601 ggggcgaggt gattcgccgc tattcgatca cctcgtcggc gcgcgcgatc aggatcgggg
     3661 aaagctcgag gccgagcgct ttcgcggtct tcagattgat cgccagctcg aactgcgtcg
     3721 gctgttcgag cggcaggtcc gcggggcggg cgccgcgcag gatgcgggcg acgaaatcgc
     3781 cggcgcgtac cccgatcgcc ctttggttgg gcccgtagga catcaacccg ccatcccgca
     3841 ccagcgcgct gaattcatag atcgcgggca gacggttggc ggcggcgaaa ttcatgatcc
     3901 gcttgcggtt gagcacggtg agcgcgtcgg aaaccatcag catggcgtcg ggccgcgcct
     3961 tggtcatctc ggcgaatgct tcgtcgaaat cgttcggctc ccggacgccg agggtctgga
     4021 cgtcgacatt gagctcgcgg gcggcattgg cggcggcctg tgagcgcagc gtcatgccga
     4081 ggtcggtggc attgaacagc atggcgacgc ggcgcaggtt cggcaccgcc tctttcagga
     4141 tctcgagccg cttgccgctg agctcggtcg acagttccgt catgccggtg acgtggccgc
     4201 ccggccgcgc caggctgtcg gcaaggccgg tggcgaccgg atcgcccgcg ccggtcacga
     4261 ccacgggaat gtcggttgcc gaggtcttgg ctgccgcggc ggcgggataa ccgaaggtga
     4321 cgatggcgtc gacgttcgcg gcctgcaggg ccgcggcaaa ttcggcgagc cgctcggatc
     4381 gcccttcgcc ggagcggaca tcgaattcga ggttgcgtcc ctcgaaaaaa ccgttcgccg
     4441 ccagtccttc caggatgctg ttacggcgct cgtccatcgc ggcgacgaca ccgccagtcg
     4501 cgagcagtcc gacccgatag gttttgcccg cctgctgcgc gcgtgcggcg cgcggccaaa
     4561 gcaaggagcc accgacgatg gcgaaaaact cgcgccgacg catgcgatgt tccgtccgga
     4621 gtgaggttga tcagactgca atgaggtggt ggcaccatat cctcccagca gcaaggcgag
     4681 aaagggcgga tcgttcacgt ccccgcgagg ctatcctgag acaggtctcc ccagacgatc
     4741 cagtaaccct ctcgcatcgt ccagatcagg caagtcgatg ccatcggtga attcatcata
     4801 gatcggcgcc agcaattttc gcgcctcggc atcgcggcct tgcccgctcc acagactggc
     4861 caggctatgc gcggcgcgga gctgccaaaa ccgcgcgttc tggctttgcg cggatttcag
     4921 cgccaagaga aagcaatcct ccgcgcgccg gcgatcggcg ggatttgcgg cgagcaggat
     4981 gtttcccttg atgcgttgag cctcggcagc ccaccagcgc tcgccggtcc gctcgatctc
     5041 ggagatcgcc tggccggcga aatcgagtgc gcgcttagaa ttgccggcgc gggcataggc
     5101 gtccgcaagg caggacaggt tgaacgagct ctggaaattc gccccggtgt tgcgatgctc
     5161 gatgagcgca gcatccatca gtgcaattcc ctcgacggcc cggccgccct cgacgaggtt
     5221 gacggccttc aaaatattgg cgcgggaaat gtggtagggg ctgcgctgct cggtggcgat
     5281 ggcaaaaccc tcttcggtcg tggcagtcgc agccggcaaa ttgcgggtgt gaaggaagaa
     5341 acgcgccgag gccaacagca ccgataccag ggtgaacgga tgaagccggc ttcgcgcgtc
     5401 gctcaccgcg gcctctctcg tttccgtcgc cagtttcagt ctgccgagat aaagataggc
     5461 gaggctgagc cagaccagcg aaaatgcccg gacgtgcgtg cccgccatga aggccaccgg
     5521 acggcaggat tcctcttcat agagcgacag ccccgtttcg aggtgaaact tcgcgtcctc
     5581 gaacctgccg atgtgcaggc aggcggcgcc ggcctcatag tgaccaatca tcgcgctcgg
     5641 cgggtcaagg cggcggcgca ggaatggctc ggccagctgc aggactttcg gcgcctccga
     5701 ccggatcaag tgatagttgc aaaggccgcc gagcgcccga agcgcttcaa agggatgcgc
     5761 ggcctcatcg agcagatcgc gcgcccttgt cagcgcggtg gcgacatcgg gcgaggcaaa
     5821 tcccttggtc atgacatagg tgttgccgag ctcgacgtgg atgcgggctt cgaattgccg
     5881 gcgcctctcc gggtcggtga tttcaggcaa gagcgtcacg gccttttcga gctgcgcctg
     5941 cgcctcgatg ccggccgaac gaacaagcga caggcgggtg gacttgagcc agtaatcgat
     6001 ggccttcccg aaagcaccgg cctcgctgca atgataggcg agcacgtccg gattgcgttc
     6061 cagcatttcg ggaaattggc gctcgaaggt ctccacgatg cgcacgtgga tctgaacgcg
     6121 ctggctcttg agcatggtgc cgtaagcggc atcctgcacc agcgcgtgct tgaaggtgta
     6181 gacggcgtgc gggacgacgc cgcgctgatg caccagctcg gaggcgacca gctgcctgag
     6241 cagcggcgcg agctcggcat cgccgagatc aaagaccgca cgaatcgagg tgtaggcaaa
     6301 ttcacgcccc agcgccgatc cggcctgcac caccgcgcgc agcgaggcga gccggtccag
     6361 ccgggcaacc agtgaagcct gcagcgtcgt gggcacggcg agaggcggca aggcctcggc
     6421 gagcacgtaa gcgccgtttt gttcgcgcag aatgtcgctt tccaggactg acttcgtcat
     6481 ctcctcgatg aacagcggaa cgccgtcggt tcgtgccacg atttgttcga gcaggaccgg
     6541 cggaaaatcc ctgccgttgg ccacctgccg gattacttcg atgttgtcgc gccggccgag
     6601 cctgttcagc gtcaacaccg tgacatgcga ctggccaagc cagggcggca caaattccgg
     6661 ccgataggtg atgatgagta gaaccggaag cgttcggatg cgctccgcca ggatgtcgaa
     6721 caattcctgc gtggtcgggt cgacccaatg cgcgtcttcg agcacgatca gcaccggctt
     6781 ctcgccggca agaccttcga tctggcccac cagttgctcc agtaacaatt ccttgcgtcg
     6841 ctgcggacta atcgacaaca cggacgaacc ctggcacgga atcgaaagca gctcggcgaa
     6901 cagggctgtg tccctcgccg gattttgcga cgacggcgcc agcagcgccg cgagtttgtc
     6961 gagcttggcc gcgtcgctgt caccggcggc aagaccggcc gcatgttcga gttgattgat
     7021 gacaggataa aacgcggtct ggctgcgatg cggcgagcac tgcaggcgca ggcaatggtg
     7081 cggctcctct ttgatcgtct cctgcagggc gaacaccagc cgcgatttgc cgatccctgg
     7141 ctcgccataa acaagcacca gccggccctc gcctgacttg atctggctcc agcgccgctg
     7201 cagcagcgcg agctcttcct cgcggccgat cagttttgtg ggcttcgaat gcagcgcttc
     7261 gaagcggctg gtggccgcgc cttccgaaag cacctgccag gcctcgaccg gatcggcgga
     7321 cgatttcaac ttgagcgatc cgagaccgcg cgtgtcgaac agcgcgccaa tcaggcgacg
     7381 ggtggttgcg gaaatcacca gcgcgcccgg ctcggcaagc gccgccagac ccgccgccag
     7441 cggcaaggca tcgccgattg cggcatggtc ggcagcaatg ccgagttcgt tcagatcgcc
     7501 gacgacgaca agcccggtcg caacggcaac gcgcgcttgc agcggcttgt cggaccgggt
     7561 gttcaaggac gcgaccgcct tgatcacggc aaggccggcg tgcaccgccc gttccgcgtc
     7621 gtcttcgcgc gcgcgcgggt atccaaaata gacgacgacc tcgccgccca aaatttggcc
     7681 gacaaaacca tcgtgctcgg tgataaccgt cttgatgcgg tcctggagag cggacatcac
     7741 ggcgagcagg tcttccgggt ctcgggccag cgaatgggcg ccggcgccgg cgaggctgca
     7801 aatcatcacc gagagttgcc ggcgctccgg ccggtcgctc ctgctgcgcg ccgcttccgg
     7861 tgcatcgttg cgcgcagcgc tcgccacctc tgcgaggcgg agcttttccc gctcgcattg
     7921 ttcccttacc tcgccgacaa agcgaaaccc cttgcgaggc agggtgcgga gcagaatctg
     7981 ccgctcgcca tcgtcaccga tggcatggcg aaccgcgttg atctgactgc tgagcgtgga
     8041 ttccgagacg atacggccgc tccagaccgc cgccatcagg tcatccttgc tgacgacgcg
     8101 ctcgcgattt cgaatgagat agagcaggag atcgaaggtc tgcggctcgg cggcgatcag
     8161 cgcagccccg cgccgcaatt cccggcggtc ggcgtcgagc agatactctt tgaacgcgta
     8221 gatcattcaa atccgccagg gcttggccgt ttcgcaaaac aaaaccagcg aattcccaag
     8281 gtaaatccaa gcatatccca aggacggccg cttccaccag cgactaaaag aagcgcctgc
     8341 cgaacgacga aaggaggcgg ccatggacgc gcttttgacg gccatcgtca tctggatttc
     8401 ggccaattac gccctgcccg cgtgcctcga tcacccgcgc atcgagcggg tagcgtcgat
     8461 tgagatggcc gggcttcgca ccaagggcct gctatcggcg cttgcgcgcc aagcaagcgt
     8521 aatcgaggtg caggaatcat ccttcgaaaa aaggcgcgac gtcgtcgccc tgtataacga
     8581 tcagaccagg acgatctacc tttcggacaa atggaccggc accacgccct cagaactatc
     8641 cgtcctcgtt catgagatgg tgcatcatct tcagaacgaa gccggcaccg cttatgaatg
     8701 tccggccgag cgcgaaaagc tcgcctacga ggcgcaggat agatggctgc gcctgttcgg
     8761 ccgcaatctt gaagccgagt tcgaggtcaa cggccttgcg ctattggtgt cgacctcctg
     8821 cggaatggcg atggggctgc actgaggacg cgatgagttt aggttgaagc ggcgaaccac
     8881 ttctccctcg ccccgctctt gcggggagag ggtcgggatg aggggctgtc tccacagacc
     8941 ggactcgcgg atagtccccc tcacccggat tgctccggac gatgcttcgc atcgccgagg
     9001 caatccgacc tctccccgca agcggggcga ggtgggccaa tacccgtgtc gattcaacta
     9061 aaggccatcg cgctttagac aaggatgcga agccgctatg cggcgttcga caaaacccga
     9121 tcagctctgc tcttcgacaa ctctcaagtt tcatcagtcg gacggtgaac cattttcacg
     9181 ctgacccaag cgcgcggcaa atcccgcgct cgaattgccg atagaccctg agcgcctgcc
     9241 ggtcggcaaa ggggaggatc tgcatcatga tcacgccggc gatccgcatc gtcgggtcga
     9301 tccagtaata ggtattgaac agaccggccc aggtgaggct gcctgccctg cggccgtcgg
     9361 gaacgggatc gatattgatc atgtgtccaa gcccccatcg cagccgaacc cccggaaaaa
     9421 aatccacgtc gttcgacagc gccgggtttg tcgtcctgag aatccccgcc tcaacgttgc
     9481 cgatctggtt ggtggacatc gaggcaacgg tctccggctg aaggatgctt gtcccgcgaa
     9541 agcttccacc attcagcagc gcctgcagca aggtcagata atccggcgcg gtggaataga
     9601 tgccgccgcc gccggaaaac gcttgcggca ccttcggctt ttccagcggc tgcggaacaa
     9661 gctttccagt cccgtccctg acatgcaggc tggcctcgcg cgcgcgctgt tgctcgccga
     9721 gcgagaagcc ggtgtcgttc atgccgagcg gcccggtaat gtgatcgctg aaatagcgat
     9781 cgaggttttg gccgccggcg atttccacca tccggccgac ccggtccagg ctgccgccat
     9841 aggcccacct cgaacccggc tcgaacatca gcggcatgcg cggcaggtgc ggattcttgc
     9901 gggcacgctt gagatagcgg ccgacatttg gatcccaaag cggatagctg aagcctgagg
     9961 tgtgcgtcag caggttgcgc agcgttaggg gtttttgcgc cgggcgcagc tgcggaacgc
    10021 ccttggcatc gaatccggtg agaatttggg ccgacgcgag cgccggatcg atattgccgg
    10081 ccggctcgtc cagcttcagc gtgccgcgct ccaccagttg cagcgcggcg accgacgtca
    10141 gcagcttgac catcgaggcg atgcggaaca ccgtgtcggt cgacatgcgc gacgccgcgg
    10201 cgatgtcgcg gaagccaaac gcgccttcat agaccaccga ctgttccttc gcggccatcg
    10261 cgacaacgcc aggtatttcc ctggcgctga cgcttgcctg caacgccctg tcgatttcct
    10321 cactgtgagc cagcttgttc agaggcgcgg cgatgccggt caaaggtgcg gccggaatca
    10381 tggcggcgga tgcaacagcc accgtccgca acaatgcatc acgtcgggtg atcggccctg
    10441 tcatttgcgt cttgaagatg tgctccatca atcggtacgt tgcggacagc cgccgccgtg
    10501 cgcagtcgcg ctcggacctc aggtgacgct ggtgcgatca tgtacccaag ttcagcgttt
    10561 ccgatcataa ttgattcgga tctatttttc gaacgccaca gcattccgac cagcgcgcaa
    10621 atcgccgcgt ccaacttatc ttgatcgctc tttctgggct tcgaaatcaa actggcgttc
    10681 ctcgcccaag tcgcgagcga aaccagtccc agcgtgacag cgtgacgatg caccatggtc
    10741 gtcacgccgg tccaatctgg caggcgaaat ctccgtccat tgccgggatt gtagcgcggg
    10801 gcaacgagcc gaccaaaata ggcggcttct agagagggaa gcgcgagcgc gggaaatgcc
    10861 tcaatcaaga agagaccttg ctcggctgtc cgtgcggcct ccgcgtcgtc acttgcgctc
    10921 aaacgttgct taaaacgcca gataggagcg tcgtcgtcaa acatcccctt cttggaacga
    10981 ttggcgggtt ggacaccgcc gccaagccag gagatgagcg aggcggcaac tttatcaacc
    11041 ggacgcatac cccctatatt gggcactatc gtcggctgat cgagcgcgac aacacagaca
    11101 tcgcatcttg attgctcggc cgcgatgaaa tcaaatgctt ggtcgaagct tgcgagttgg
    11161 ggattgcttg atgataaatg cccgtgatgg tcgcaacgga tcgcgcaaac tgcgccaagt
    11221 gcctttgggt tgtccgtcca agccgagtcg aaaccgacca ccgtccaact aaccatcgat
    11281 cacctcttta ttgatcccta ctagctcaat tacctccacg ttacactcgc caccgggagg
    11341 cgcggtgtcg aaacgtcatg cgcggaaata tcgcttgtgg ccgttatcca tttcatagcg
    11401 caccgcgatg ccgctgagtt gggtctgctt cttgaccgag atggtctgga agccatgggc
    11461 ttggatcagc gcgctcaaac agaaagaaga cgtggcgatc gggctgtaga atgcgccgaa
    11521 tacacctcgc ccgccggttc accggcgaga acttcgaaaa agcagttgct gaaaattgcc
    11581 gatccgcctc ttggacgact aaattgaaca cggacagccg tccgtcgcag aatgatcatg
    11641 atttcggcgt actcgccggg ctggctatcg acttttatag agccgcttga cctgaaaccg
    11701 ggtgggctca aggcttgatt atatgagcgt tctgagccgt cgtgtcctga ttacgggtgt
    11761 ttctgccacc ggtgcgatgt tgggtctcac cgggtgcgcg agcacgccgg cccgcgtggc
    11821 ggaggtcgat ccagcctacc gccggacaga cgttgcgtac gccacgcggg agccgcgcgg
    11881 caccatcgtt gtcgatccgc tgcagcatta cctctactac gtggagggcg gtggacaagc
    11941 ccggcgttat ggaattgccg tcggcggcga aggatttggc tggtctggcg tggccacggt
    12001 ccacagcaag caggaatggc cggactggta tcccaccagg gaaattctcg aacgccaccc
    12061 cgagctcaca gccgggttga aggaattgca gggcggccag ggcatgcccg gagggcccga
    12121 cagcccactc ggtgcccgcg cgctttacct gtggcagggt aacaaggaca ccctgtatcg
    12181 catccatggc acaaacgaac cctggaagat cggcagcaac gtttcctccg gctgtatccg
    12241 aatgatcaat gacgacgcga tcgatctcta cgatcgtgtg cggatcaaca ccagggtggt
    12301 cgtgctggca tcgaacgtag gttgatggcg cgctgcatgg gttgagcgat gcgatcccca
    12361 tctgttcgga acgagggtga tggtctcgcg cagggctcaa tccatcctgc gctctatggc
    12421 tcctcgcgat gacaggtacg cgaaaactgc ccgacgggca aatcacttct gattttcaga
    12481 aatcgtgtca agccccgttt gcaaaaatat ttctgttttc ctttgaccca aatcagttca
    12541 ctgattcgcc ccgtcccgtt ccgacagagg ggcgttgcgc aacgtcatca acgtggagcg
    12601 ggatgcggtg gacgcgggca gcgcgcgaga cgaacgcggt ctgctacgta cggtgaagtc
    12661 gtgtggttcc gacgccccaa tcgttggcgt caagtctgcg ataagaagcg caggcgacgg
    12721 tgtcaaaaag cgtggtcacc gaggagagcg cgaagtaagc cgtaaaacca ttgcgcgggg
    12781 aatgccg
//
LOCUS       DATNOI010000657         6841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1656293,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000657 DATNOI010000000
VERSION     DATNOI010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656293"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..3305)
                     /locus_tag="VN968_26460"
     CDS             complement(30..3305)
                     /locus_tag="VN968_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HXL32810.1"
                     /translation="MSIFVALHHVTHYKYDRPIDLGPQTIRLRPAPHTRTPILSYSLK
                     VTPSNHFVNWQQDPQGNWLARFVFPEKATELRIEVDFTAQMTVINPFDFFVEPYADGF
                     PFAYTNDLKTELAPYLATIEPGPLFLKYLADIPREATSTVNFLVELNAQLQKKIRYII
                     RMEPGVQTPEQTLAAGAGSCRDSAWLLIQILRHLGLAARFVSGYLIQLRPDIDPVEGP
                     REIENDFTDLHAWAEVYLPGAGWIGFDVTSGMLTGEGHIPVAATPHYRTAAPISGSAG
                     FANVEFGFEMNVRRIREAPRITKPFSDDSWALLDRLGEQVDADLKAGDVRLTMGGEPT
                     FVSVDDLESPEWNIAAVGPTKRGLADDLIRRLRTRFAPGGLLHYGQGKWYPGESLPRW
                     AFGLYWRKDGVPIWKNSDLIATIESRRKARIEDAERFAEGAAKKLGVDSEFIMPAFED
                     PGHWLQKEAALPPNVDPSDSRLSDPEERSRMARVFDQGLNVPKGFVLPIQRWNADAGR
                     WRSERWKLRRGNLFLTPGDSPLGLRLPIASLPHIPAEEYPYIVEQDPLEPRDTLPVYD
                     GTAPSETAPHQEVQEQQLKGGGVRTAMSIEIRDGILCAFMPPVEKLEDYLELITAVEA
                     TAEELQMQVHVEGYPPPFDPRVEVIKVTPDPGVIEVNIQPAASWRDAVDITLGLYEDA
                     AQCRLGANKYLVDGRHTGTGGGNHVVVGGSMPADSPFLRRPDLLKSLVLYWQRHPSLS
                     YLFSGMFIGPTSQAPRIDEARHDGLHELEIALAHVPPPGIKAPLWLVDRLFRHILVDI
                     TGNTHRAEICIDKLYSPDGPTGRLGLVEFRALEMPPDPRMSLAQQLLIRALIAKLWRE
                     PQQGKFVRWGTTLHDRFMLPHFLWEDFLGVLTELKQSGYDFSPDWYAAQLEFRFPVFG
                     RVHHGGVALEVRQALEPWHVLGEEGSAGGTVRYVDSSVERLQVKASGFVEGRHVVTCN
                     GRRMPMTETGRSGEAVAAVRFKAWQPASGLHPTIPVHAPLTFDLIDTWNGRSLGGCVY
                     HVAHPGGRTYDSKPVNSYEAEARRLARFQDHGHSPGKIDPPREERTIEFPLTLDLRRP
                     LLH"
     gene            complement(3451..3960)
                     /locus_tag="VN968_26465"
     CDS             complement(3451..3960)
                     /locus_tag="VN968_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HXL32811.1"
                     /translation="MRSRAIRFVTLGCLALLAMLNIGIVSADAATIVALGASNTYGKG
                     VARNQAYPAQLEAILRAKGSNVHVVNAGINGDTTEGMLGRLDRAVPNGTSAVILQPGG
                     NDRRKGRADRTADIQSRLSARGIPVIMLGNGMLRGLPHQPDGQHLTPEGYHMLAESLA
                     SQVSGAIGR"
     gene            complement(4057..5580)
                     /locus_tag="VN968_26470"
     CDS             complement(4057..5580)
                     /locus_tag="VN968_26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HXL32812.1"
                     /translation="MAPAPVIRLHPDDGVLIARSSLPPGMVVVDGVTTVERIPAGHKV
                     AIRSIAIGEPIRRYGQIIGFATAPIAPGQHVHTQNCGMGDFAKDYAFGVDVTATPNFD
                     LPATFDGIRRPDGRVATRNYIGILTSVNCSAHVAGMVADVFKKNPFTHDNPLADFPNV
                     DGVVALTHKTGCGMTQDEPLTVLRRTLGGYARHANFAAVVVLGLGCEVNQIGGLMKEQ
                     KLAGRLRELEIQEIGGTRKTVEAGVAFVREALAEANNVKREPVSASELTVALQCGGSD
                     GYSGVSANPALGAASDLLVRHGGTVILSETPETYGAEHLLTRRAVSREVGEKLVALMR
                     WWEEYTEREGAEMNANPSPGNKAGGLTTILEKSLGAMAKAGSTNLVDVVNYAEAVTKK
                     GFVFMDTPGYDPVAATGQVAGGANLVCFTTGRGSVFGCKPAPSIKLATNSAMYKRMED
                     DMDVNCGTILDGEETVQQCGQRIFDLMLKTASGQPTKSESFDFGGAEFAPWVLGATM"
     gene            complement(5758..6537)
                     /locus_tag="VN968_26475"
     CDS             complement(5758..6537)
                     /locus_tag="VN968_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXL32813.1"
                     /translation="MATNNVKKIWASGKAVVNAWLAIPSGFSAEVIAQCGFDSVTVDM
                     QHGVQDYLSMVQCFQAMNGHPVTPMVRVPWNEPGIIGKVLDGGAYGVICPMVNTKEEA
                     EKLVAYSKYPPRGTRSNGPIRSGMYGSAGTYQATANDEIVLLPMMETKTAVENMESIL
                     DVEGINGVYIGPSDLGFSYGLVPKLDREEPEILKIYERIVKECGKRGLYPGIHCSGAV
                     GAVRAIDMGFKLVTLSNESGLMMTYAKMQVAQTRKDSGGKA"
     gene            complement(6691..>6841)
                     /locus_tag="VN968_26480"
     CDS             complement(6691..>6841)
                     /locus_tag="VN968_26480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HXL32814.1"
                     /translation="VLIPGEPEAKMRAERTKNGVPLPDDTWAAIVNTAREVGVSEASI
                     QRAVE"
BASE COUNT         1286 a         2193 c         2060 g         1302 t
ORIGIN      
        1 atcccgactc tcctgcccaa agcctatcct caatgaagga gcggcctcct caagtcgagg
       61 gtcaaaggaa attcaattgt gcgttcctcg cgcggcgggt cgatcttgcc ggggctatgg
      121 ccatgatcct gaaaccgggc aagccgccgc gcctcggcct cataggaatt gaccggtttg
      181 ctatcataag tgcgtccgcc ggggtgggcg acgtgataga cgcagccgcc cagcgagcgg
      241 ccgttccagg tatcgatcag atcaaacgtc agcggcgcat gaaccggtat cgtcgggtgc
      301 agcccggagg ccggctgcca ggccttgaag cggacggccg cgaccgcttc tcccgaacgg
      361 ccggtttccg tcatcggcat ccgccggccg ttgcaggtga cgacgtggcg accttcgaca
      421 aagccagagg ccttgacctg caaccgctcc accgatgaat cgacatagcg cacggtgccg
      481 ccggccgagc cctcttcgcc aagaacgtgc cacggctcca gcgcctgccg cacttccagc
      541 gcgacgccgc catggtgcac ccggccgaac actggaaacc ggaattcaag ctgcgcggca
      601 taccaatccg gcgaaaaatc gtaaccggat tgttttagct ccgtgagcac gcccagaaag
      661 tcttcccaga ggaaatgcgg cagcatgaag cggtcgtgca acgtcgtgcc ccagcgcacg
      721 aatttgcctt gctgcggctc gcgccagagc tttgcgatca acgcccggat cagcaattgc
      781 tgcgcgagag acatgcgggg gtccggcggc atttccaatg cacggaattc gaccaaccca
      841 agccggccgg tcgggccatc gggagaatac agcttgtcga tgcagatctc ggcgcgatgg
      901 gtattgccgg tgatatcgac caggatgtgc cggaacagcc gatccaccag ccacagcggc
      961 gccttgattc ccggcggcgg cacgtgggcc agcgcgatct caagttcatg gaggccgtcg
     1021 tggcgcgctt cgtcgatacg cggcgcctgg ctggtcggac cgatgaacat gccggagaac
     1081 aaataagaca gcgacggatg ccgctgccag tacagcacca ggctcttcag caagtccgga
     1141 cggcgcagga acggtgaatc cgcaggcata gaaccgccga ccaccacatg attgccgccg
     1201 ccggtgccgg tgtggcggcc gtcgacaaga tatttgttgg caccgaggcg acactgcgcg
     1261 gcgtcctcgt aaaggcccaa ggtgatatcg acagcgtcgc gccagcttgc cgccggctgg
     1321 atattgacct cgatcacgcc gggatcgggc gtcaccttga tgacctcgac acgcgggtcg
     1381 aacggcggcg gatagccctc gacatgcacc tgcatctgca attcctcggc ggtcgcctcg
     1441 accgccgtga tcaattccag atagtcttcg agcttctcca ccggcggcat gaatgcgcac
     1501 aaaatgccgt cgcggatctc gatcgacatc gcggtgcgca cgccgccgcc cttgagctgc
     1561 tgctcctgca cctcctgatg cggcgcggtt tcggacggcg ccgtcccgtc gtacaccggc
     1621 aatgtgtcgc gcggctccag cgggtcctgc tccacgatat agggatattc ctccgccgga
     1681 atgtgcggca acgaggcgat cggcagtcgc agccccaacg gggaatcgcc cggcgtgaga
     1741 aacagattgc cgcgccgcaa tttccagcgc tcgctgcgcc agcgtccggc gtcggcgttc
     1801 cagcgctgga tcggcaagac gaaacccttg gggacgttga ggccctggtc gaatacccgc
     1861 gccatccgcg agcgctcttc cggatcggac aatctggaat cgctcggatc gacattcggc
     1921 ggcaacgccg cttccttctg cagccaatgg cccgggtctt cgaacgcggg catgatgaac
     1981 tcggaatcga cgccgagttt ttttgcggca ccttccgcaa agcgttcggc gtcttcaatc
     2041 ctggccttgc gccggctctc gatcgtcgcg atcagatcgg aattcttcca gatcggcacg
     2101 ccgtccttgc gccaataaag accgaacgcc cagcgcggca ggctttcgcc tggataccat
     2161 ttgccctgcc cgtaatgcag caggccgccg ggcgcgaacc gtgtccgcaa gcgtcgaata
     2221 agatcgtcgg caaggccgcg cttggtcggt ccaaccgcgg cgatgttcca ttccggcgat
     2281 tccagatcgt cgaccgaaac gaaggtcggt tcgccgccca tcgtcagtcg tacgtcgccc
     2341 gccttcaaat cggcatcgac ctgctcgcct aacctgtcga gaagcgccca ggaatcgtcg
     2401 gagaacggtt ttgtaattcg cggcgcttcg cggatccgcc tgacgttcat ctcgaagccg
     2461 aattcgacgt tggcaaaccc ggcgctgcca ctgatcggcg ccgccgtgcg ataatgcgga
     2521 gtggcagcga cgggaatgtg cccctcgccg gtcagcatgc ccgaggtgac gtcgaacccg
     2581 atccagcccg caccgggcag atagacttcg gcccaggcgt gcaaatcggt aaaatcgttc
     2641 tcgatctcgc gcggtccttc cacgggatcg atatcggggc gcaattgaat gaggtagccc
     2701 gagacgaagc gggcggctag cccgagatgg cgcaggattt gaatcaacag ccacgccgag
     2761 tcacggcacg atccggcacc cgcggccaac gtctgctcgg gcgtctggac gcccggctcc
     2821 atgcggatga tgtagcgaat tttcttctgc agttgcgcgt tgagctcgac caggaaattc
     2881 accgtgctgg tggcctcgcg gggaatatcg gcgagatatt tcaaaaacag cggcccgggc
     2941 tcgatggtcg caagataggg cgcgagttcg gtcttgagat cgttggtata ggcgaaggga
     3001 aaaccgtcgg cgtagggctc gacaaagaaa tcgaacggat tgatcacggt catctgcgcc
     3061 gtgaaatcga cctcgatcct gagctcggtg gccttttccg ggaagacaaa gcgcgccagc
     3121 cagttgccct gcgggtcctg ctgccagttc acgaaatggt tcgatggggt aaccttgagc
     3181 gaatagctca ggatcggggt gcgcgtgtgc ggtgccgggc gcaggcgaat ggtttgaggg
     3241 ccgagatcga tcggacgatc atacttgtag tgcgtgacgt gatgtagcgc gacgaagatc
     3301 gacacgggca agccactcca gcagcttttt taagcagaac actggttccg gctgacatca
     3361 agcactaaca acgggcaagg gttgcccagg aactatccgc atgtcgtccc ggcgaattca
     3421 ggaatgagcg cggtttgtcg ccctcaacgg ctagcggccg atcgcacctg atacctgcga
     3481 agcgagcgac tcagcgagca tatggtagcc ctccggggtt aaatgctggc cgtcgggctg
     3541 gtgtggcaat ccgcgcagca tgccgtttcc gagcatgatc accgggatgc ctcgggcgct
     3601 caggcgactt tgaatatccg ccgtgcgatc agcccggccc ttacgccgat cattgccgcc
     3661 gggctgcaaa ataacggcac tcgtcccgtt cggcacggcc cggtccaagc gtcccaacat
     3721 cccttccgta gtatcgccgt tgatgccggc gttcaccaca tggacgttcg accctttcgc
     3781 ccgcaatatc gcttcgagtt gcgcgggata agcctgatta cgcgccactc ccttgccata
     3841 agtattgctc gcaccaaggg ccacgattgt tgcggcgtca gccgatacaa tgccgatatt
     3901 caacattgca agtaatgcca agcacccaag agtcacgaac cgaatcgcgc gcgatctcat
     3961 gtttcctccc ttaaaggcca cttcgcaggg ctgacatcgt ccgccgtaaa tggcctcacc
     4021 cgttgaaggc aaaacaatcg atgatctcgc cacatatcac attgtcgcgc caagcaccca
     4081 gggcgcgaat tccgcgccgc cgaaatcgaa gctctcgctc ttggtcggct gtccggaggc
     4141 tgtcttcagc atcaggtcga aaatgcgctg gccgcattgc tgcacggttt cctcgccgtc
     4201 gaggatggtg ccgcaattga cgtccatgtc gtcttccatg cgcttgtaca tcgccgagtt
     4261 ggtcgcaagc ttgatcgagg gtgcgggctt gcagccgaac acgctgccgc ggccggtggt
     4321 gaagcagacg agattggcgc ccccggccac ctgcccggtc gccgccaccg ggtcgtagcc
     4381 cggcgtatcc atgaagacaa aacccttctt ggtgacggct tcggcgtagt tcaccacatc
     4441 caccagattg gtgctgccgg ccttggccat cgcgccgagc gacttttcca ggatcgtggt
     4501 gagcccgccg gccttgttgc cggggcttgg attggcgttc atctcggcgc cctcgcgctc
     4561 ggtgtattct tcccaccagc gcatcagcgc caccagtttt tcgccgacct cgcggctgac
     4621 cgcacggcgt gtcaacagat gctctgcgcc ataggtctca ggcgtctccg acaggatcac
     4681 ggtgccgccg tgccggacca gcagatcgct cgcggcaccc agcgccggat tggcggaaac
     4741 gcctgaataa ccgtcggaac cgccgcattg cagcgccacc gtcagctcgc tcgccgacac
     4801 cggttcgcgc ttcacattgt tcgcctcagc gagcgcctcg cgcacgaagg cgacgccggc
     4861 ttcgacggtc ttgcgggtgc cgccgatttc ctgaatttcc aactcgcgca ggcggcctgc
     4921 gagcttctgc tctttcatca gcccgccgat ctggttgacc tcgcagccca ggcccagcac
     4981 gaccacggct gcaaaattcg catgccgtgc gtagccgccg agcgtccggc gcagcaccgt
     5041 gagcggttcg tcctgcgtca tgccgcagcc ggtcttgtgg gtcagcgcga ccacgccatc
     5101 gacattgggg aaatcagcca atggattgtc gtgcgtgaac ggatttttct tgaagacatc
     5161 cgcgaccatg ccggcgacat gcgcgctgca attcaccgag gtgaggatgc cgatatagtt
     5221 gcgggtcgcc acccgcccgt cgggacgacg gatgccgtca aaggtcgcgg gcagatcgaa
     5281 gttcggcgtc gccgtgacgt cgacgccgaa cgcgtagtcc ttggcgaaat cgcccatgcc
     5341 gcagttctgg gtatggacat gctggcccgg cgcaatcggc gcggtcgcga agccgatgat
     5401 ctggccgtag cggcggatcg gctcgccgat cgcgatcgag cggatcgcaa ccttgtggcc
     5461 cgccggaatc cgttccaccg tggtgacgcc gtcgaccacg accatgccgg gtggcaggct
     5521 cgagcgcgcg atcagcacgc catcatcggg atgcagtcga atgacgggtg ctggggccat
     5581 gtcgatgtct ccttttctcg ctttcacctc tccccgttct ttacggggag aggtcggact
     5641 gcgaagcagt ccgggtgagg ggcaaggcac aatgtcaagc gcgaccacag aactcgcgga
     5701 aagaagcccc tcaccccgac cctctccccg cgaagagcgg ggcgagggag aaggaagtca
     5761 cgccttgccg ccggaatcct tgcgggtctg cgcgacctgc atcttggcgt aggtcatcat
     5821 caatccgctt tcgttcgaca gcgtcacgag cttgaaaccc atatcgatcg cgcgcaccgc
     5881 gccaacggcc ccgctgcagt gaatgccggg atagagcccg cgcttgccgc attccttcac
     5941 gatcctctca tagatcttga ggatctccgg ctcttcacga tcgagttttg ggaccaggcc
     6001 ataggagaag ccgagatcgg acggaccgat atagacgccg ttgatgcctt cgacatcgag
     6061 gatcgattcc atgttctcga ccgccgtttt ggtctccatc atcgggagca gcacaatctc
     6121 gtcatttgcg gtggcctgat aggtgccggc cgaaccatac atgccggaac ggatcgggcc
     6181 gttcgaacgc gtgccgcgcg gcggatattt ggagtaggcg acgagctttt ccgcctcctc
     6241 tttggtgttg accatcggac aaatcacgcc ataggcgccg ccgtcgagca ccttgccgat
     6301 gatgcccggt tcattccagg gcacgcgcac catcggcgtc accggatgac cattcatcgc
     6361 ctggaagcat tgtaccatcg acaggtagtc ctgcacgccg tgctgcatat cgacggtgac
     6421 gctgtcgaag ccgcactgcg cgatcacctc ggcggaaaac cctgaaggaa ttgcaagcca
     6481 ggcgttcacc acggccttgc ccgaggccca tattttcttg acgttgtttg ttgccactgt
     6541 gtttccttcc gttttgccca gacacaccgt cattgcgagg agccgacggg ttccgcctct
     6601 ggcgggcccg atgataaact ccgcgacgaa gcaatccatt ctgtgcttgc gactttggat
     6661 tgcttcgctt cgctcgcaat gacgccaaaa ctactccacg gcccgctgta tgctcgcctc
     6721 gctcacgccg acttcgcggg cggtattcac tatcgccgcc caggtatcgt caggcaaggg
     6781 cacaccgttt ttggtgcgct cggcgcgcat ttttgcttcc ggctcgcccg ggatcagcac
     6841 g
//
LOCUS       DATNOI010000658         2234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1980032,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000658 DATNOI010000000
VERSION     DATNOI010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980032"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VN968_26485"
     CDS             <1..930
                     /locus_tag="VN968_26485"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL32815.1"
                     /translation="PLISCVSPRPPVAVSRHTIVSATTWQVLMSPQASFAASLRRWRR
                     HRGLSQLDLAAATEISQRHLSFMELGRASPSRDMVMRLAAALDVPLRQHIALLISAGF
                     APVWRQTDLAAPELREVSSALDYMLAQQEPFPAVAVDRHWNLLRSNSGAVRLIEFLVG
                     PLAPGTAVNMADALVSPVVLRPHLVNWADVVRYFVRSVEADAAADGTPETAALHERLL
                     GYDGVRTAINATPAELAMTPVLPMHFRKGNTSLRLFTTIATLGTPQDVTLQELRVECF
                     FPMDDESAGVMRGWSAESQSMPTQGRAISSPQS"
     gene            complement(921..1733)
                     /locus_tag="VN968_26490"
     CDS             complement(921..1733)
                     /locus_tag="VN968_26490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HXL32816.1"
                     /translation="MLAIVLAVGSSADGALAQTGKTGVEKLYVLNCGEGVAGDISRWS
                     PGVNEGKSMDFVDNCYLIKHAQGWFLWDTGISDAVAAMPNGLAPSDPKAVFWRRPKTL
                     AAQLDQLGVTPADVKAMAVSHTHPDHIGNVEMFPTTMLYVQKAEYEWPAANNEPRFKP
                     AHPVTKLEGDGDVFGDGSVTILSTPGHTPGHQSLLVKLPHTGAVVLSGDAVHFKSNWD
                     NRGVPALNFSKEQTLASMQKISDTLAKENAQLWINHDKAQRDGLKMSPEFYD"
     gene            complement(1898..>2234)
                     /locus_tag="VN968_26495"
     CDS             complement(1898..>2234)
                     /locus_tag="VN968_26495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HXL32817.1"
                     /translation="WVWADPAVGIIGSLVIASWAFGLIRDSGAVLLDVSVDKNLEAVI
                     RDRLETRGDRVTDLHLWQVGPGHRAAVISVISDHPLPSATYKRRLNGLRGLSHVTIEV
                     ELCPHPPLH"
BASE COUNT          405 a          703 c          687 g          439 t
ORIGIN      
        1 ccccttatct cctgtgtctc gccgcgcccg cctgtggcag tgtcgcggca caccatcgtc
       61 tccgcaacta cctggcaggt actcatgtcg ccgcaagcaa gctttgcagc cagcctccgc
      121 cgctggcgcc ggcaccgggg cctgtcgcag ctcgatctgg ccgccgctac ggaaatctcg
      181 cagcggcatc tgagcttcat ggaattgggc cgcgcgtcgc ctagccgtga catggtgatg
      241 cggcttgcgg ccgccctcga cgtgccgctg cgccagcaca ttgcgctgct gatatccgcc
      301 ggctttgctc cggtttggcg gcagaccgac cttgccgcgc cggaacttcg cgaagtcagc
      361 agcgcgctcg attacatgtt ggcccaacag gagccgtttc ccgccgtcgc ggtcgaccgg
      421 cactggaatt tgctgcgcag caattcgggg gcggtacgcc tgatcgaatt cctggtcggt
      481 ccattggcgc cgggcacggc ggtcaatatg gccgatgcgc tggtctcacc cgtcgtgctg
      541 cggccgcatc tggtgaactg ggcggacgtg gtccgctatt tcgtccgcag cgtggaagct
      601 gacgccgccg ctgacggcac gcccgagacc gcggcacttc acgaacgact gctcggctat
      661 gacggggtgc ggacggccat caacgccacg ccagccgaat tggcgatgac gccggtgctt
      721 cccatgcatt tccgcaaggg caacacgagc ttgcggttgt tcacgaccat cgcgacgctg
      781 ggaactccgc aggacgtcac gctgcaagag ttgcgcgtcg aatgcttttt tccgatggat
      841 gatgagagcg ccggcgtcat gcgcggttgg agtgcagaga gccaatccat gccaacacaa
      901 ggcagagcga tcagctctcc tcagtcgtag aactcaggcg acatcttgag gccgtcgcgc
      961 tgcgccttgt cgtgattgat ccagagctgc gcgttttctt ttgcaagcgt atccgatatc
     1021 ttttgcatgg aggccagcgt ctgctccttg ctgaagttga gggcgggaac cccacgattg
     1081 tcccaattgc tcttgaaatg gacagcatcg ccggaaagaa cgaccgcgcc ggtgtgaggc
     1141 agcttcacca ataatgattg atgacccggc gtatgtcccg gggtcgagag aatggtcacg
     1201 ctgccgtcgc cgaatacgtc accgtcgcct tccagttttg tgacgggatg cgccggcttg
     1261 aaccgaggct cgttattggc agcgggccat tcgtattcgg ccttctgtac gtagagcatc
     1321 gtggtgggaa acatctcgac attcccgata tggtcgggat gggtgtgcga gaccgccatc
     1381 gccttgacgt cggctggggt gacgccaagt tgatcgagtt gagccgccaa cgtctttggc
     1441 cggcgccaga acacggcctt cggatccgat ggcgcaaggc cattcggcat ggccgcgacc
     1501 gcatccgata tcccggtgtc ccacaggaac cagccttgtg cgtgtttgat cagatagcag
     1561 ttgtcgacga agtccatgga tttgccttcg ttgactcccg gagaccaccg cgaaatatcg
     1621 ccggcgacgc cttcaccgca attgagaacg taaagttttt cgacgccggt cttgcccgtt
     1681 tgcgccaggg cgccatcggc ggaagaccca acggccaaaa cgatcgctaa cactccacat
     1741 actcggttca cgtcatctct cccatggtcg gcaaacccgg atgttcagcc gttgacgcgg
     1801 acgttagtcc atcgcgaatg ttccatccaa atgacgattg cgcgaagctt ccgccgcgca
     1861 gcgccgctat cgcgcgaccg gtcgcgggac aagaagctca atgcagcggc ggatgcggac
     1921 agagctcgac ctcgatcgtc acatggctga gacctcgcaa gccgttgagg cggcgcttgt
     1981 aggtcgccga aggcagcggg tggtcggaga tgacggatat caccgctgcg cgatggcctg
     2041 gaccgacctg ccacagatgc agatcggtaa cccggtcgcc cctggtctcc agccggtcac
     2101 ggatcacggc ttcgaggttt ttgtcgacgc tgacatcgag cagcacggcg ccggaatcgc
     2161 ggatcagccc gaacgcccaa ctggcgatga cgaggctgcc gatgatcccg accgccggat
     2221 cggcccagac ccat
//
LOCUS       DATNOI010000659         4522 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1855667,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000659 DATNOI010000000
VERSION     DATNOI010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855667"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VN968_26500"
     CDS             <1..631
                     /locus_tag="VN968_26500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL32818.1"
                     /translation="AKAIGGAALGLRCDVTDAASVKDAFAQVASAFGGVGIAVSNAGA
                     AWQGRIGEVDEAVLRESFELNFYGHQRVAQAAVKIMRAQGTGGCLLFNVSKQAVNPGP
                     DFGPYGLPKAATLFLVRQYALDYGGEGIRANAVNADRIRSGLLTEEMIASRSKARGLS
                     EQAYMRGNLLGREVAAEDVAQAFLAQALALKTTADVTTVDGGNIAAALR"
     gene            734..913
                     /locus_tag="VN968_26505"
     CDS             734..913
                     /locus_tag="VN968_26505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HXL32819.1"
                     /translation="MSEEPYKSWQCRTCGYIYDEAAGAPDEGLAPGTRWVDIPDDWVC
                     PECGTPKSDFDMVEL"
     gene            complement(921..1562)
                     /locus_tag="VN968_26510"
     CDS             complement(921..1562)
                     /locus_tag="VN968_26510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXL32820.1"
                     /translation="MIEAVIWDFGGVFTTSPFEAFARFEIERGLPADIIRRTNASNHL
                     ENAWAKFERAEVDIETFDQLFAAESLALGAEVRGKDVLPLLAGDLRPEMVAAFKRIAA
                     KLKTGCITNNLPANAIGSLGGRSIYVAEVMALFDHVIESAKIGLRKPDPRIYQMMVEA
                     LHVDPKRCVYLDDLGVNLKPAREMGMTTIKVVNAPQAIAELEAATGLALRSRP"
     gene            complement(1841..2068)
                     /locus_tag="VN968_26515"
     CDS             complement(1841..2068)
                     /locus_tag="VN968_26515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32821.1"
                     /translation="MNAISFGAVAHERPGTTADEPNSDKSPDAAWIVLEAANDLGDHA
                     TVEACRRVIDANLNGTLASEADLHVIFNYFR"
     gene            complement(2114..3229)
                     /locus_tag="VN968_26520"
     CDS             complement(2114..3229)
                     /locus_tag="VN968_26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HXL32822.1"
                     /translation="MTEVKSFLDDLDDAVARGTAESRKRALWHATDLLIAGRYSDDEI
                     WTFGEVIGRLADEIEVAARAQLAKRLANFDNAPINIIHKLAFDDSIDVAGPVLRESEQ
                     LDDNALIANATTKSQSHLLAISQRKSIGETVTDVLVARGDRDVVNSIASNAGARLSDF
                     GFLHMVKRAEGDSILAEQLGLRGDIPRRVFQQLIAKASEDVKKRLERERPAMVDQIQT
                     SVTDVAGALQSKFGPVSRSYFVAKRLVTTQHQQGNLNENSISGYAQSHRFEEVTIGLS
                     LLCSLPADVIERALADKNREMLLVLAKALDFSWQTTMSLLFLGAKDHRITAQDLNDLE
                     REFGRLNLETSRSVLKFYQTRKSAAGTESRAEAPAGA"
     gene            3426..4169
                     /locus_tag="VN968_26525"
     CDS             3426..4169
                     /locus_tag="VN968_26525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HXL32823.1"
                     /translation="MIAVLALFLSAPADAIVGGGVPSAEGVGRSAVTIVGSRGNFCTG
                     ALIASRLVLTAAHCVQPGAVYKIVEYGADKQPQLRDVKNVAIHPAFNMQAIMAHRATA
                     DVALLQLEAPPNGKNAADLGAPQVPIVAGSRFTIAGIGVAIRGEGKSGGTVRVAGLVA
                     TGQPGTLQIRLVDPVGQGAREGLGACTGDSGGPVFEDRHGAPAIVGVISWSTGPNGSA
                     GCGGLTGVTPLTLYRDWIVQTARQWGFAL"
     gene            complement(4195..>4522)
                     /locus_tag="VN968_26530"
     CDS             complement(4195..>4522)
                     /locus_tag="VN968_26530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32824.1"
                     /translation="KYGALSTLSGRLSIKWETQEDVLRMVWEEKDGPVVERPVARGFG
                     TRSVIASIESQLGGQAEFDWRSEGLVCRLSVPLMQDAAARQLNNLQNAIVGRNGAELA
                     SRSADA"
BASE COUNT          854 a         1391 c         1370 g          907 t
ORIGIN      
        1 ggcaaaagcg atcggcggcg cggcacttgg gcttcgatgc gacgtcaccg acgcagcctc
       61 ggtcaaggat gcgtttgcgc aagtcgcctc ggccttcggc ggggtcggca tcgcggtgtc
      121 gaacgccggc gccgcctggc aggggcggat cggcgaggtc gacgaggcgg tgttacgaga
      181 gagttttgaa ttgaattttt atggccacca gcgtgtcgcg caggctgccg tcaagatcat
      241 gcgcgcgcag ggcacgggag gctgcctgct gttcaatgtt tccaaacagg cggtcaatcc
      301 cggacccgac ttcggcccct atggcctgcc gaaggcggcg accctgtttc tggtgcggca
      361 atacgcgctc gattacggcg gcgaaggcat tcgcgccaac gccgtcaatg ccgaccggat
      421 ccgttccggc ctgctgaccg aagagatgat cgcgtcacgc tcaaaagccc gcggtctcag
      481 cgagcaggcc tatatgcggg gcaatctcct cggccgcgaa gtcgcggccg aggatgtcgc
      541 gcaggccttt ctcgcccagg cgctggcgct gaagaccacc gccgacgtga ccaccgtcga
      601 cggcggcaac atcgccgctg cgctgcggtg aggggggtgg aagttgctgc ggcagccctc
      661 tggtcttcgg agaacctgcc ctctacactc gtgagacgtc ccggccacga accgtgcaag
      721 accgagtatc cgaatgagtg aggagcccta caaatcctgg cagtgccgga cttgcggcta
      781 catctatgac gaagcagccg gcgcaccgga cgaaggcctg gctccgggca ccaggtgggt
      841 cgacattcca gacgactggg tttgtcctga atgcggcacg cccaagagcg acttcgacat
      901 ggtggagctg tagacacaag tcatggacgc gaacgcagtg ccaaccctgt cgccgcttcg
      961 agctcggcga tggcttgcgg cgcattgacg accttgatcg tggtcatgcc catttcgcgc
     1021 gccggcttca ggttgacgcc gagatcgtcg agatagacgc aacgcttggg atcgacgtga
     1081 agtgcttcca ccatcatctg atagatgcgc ggatcaggct tccttaagcc gatttttgcc
     1141 gactcgatga cgtgatcgaa cagcgccatc acctcggcga catagatcga tcggccgccc
     1201 aggctaccga tcgcgttggc gggtaggttg ttggtgatgc acccggtctt gagcttggcc
     1261 gcgatacgct tgaaggccgc gaccatctcg ggccgcaaat cccccgccag aagcggcaag
     1321 acgtccttgc ctcgaacttc cgcgcctaat gccaacgatt ctgcggcgaa caattgatcg
     1381 aacgtctcaa tatccacctc cgcgcgctcg aatttcgccc aggcattttc cagatgatta
     1441 ctggcattgg tgcgccggat gatatctgcg ggcagcccgc gttcgatctc gaaacgcgca
     1501 aaagcctcga aaggcgaggt ggtgaacacc ccaccgaaat cccagatcac tgcctcgatc
     1561 atccatttcc tgccaatgcg agcgcgattt ggaaattggc taacacggtg tccgggcgcg
     1621 caccagccct gaggcgagca agtccgattt ttggcgcccg atgtttcgcg ctcctcagcg
     1681 tctgcctcaa atgcagccga tggaatgggg cgttctagta aagctcgtca cgggcatcca
     1741 cgtcctagct gcatccaggc aaaagacgtg gatggccggg acgagcccgg ccatgacgaa
     1801 aatcgaacca acttcgggta gttcaggacc cgtctgcaat tcacctgaaa taattgaaga
     1861 tgacgtgcaa gtcggcctcg gaggcaagcg tgccgttcag gttcgcgtcg atgacacggc
     1921 gacaggcctc gacggtcgca tgatccccga gatcgttcgc tgcctccaga acgatccagg
     1981 ccgcatctgg ggatttgtcc gagttcggtt catcggcagt tgtgccgggg cgctcgtgtg
     2041 cgactgcgcc gaatgagatt gcgttcattt gcgatgtttc cctgccattc cagatcggta
     2101 atctcaatgc acgtcaagcg ccggcaggcg cctcggcccg ggactcggta cctgcggcac
     2161 tcttgcgtgt ctgatagaat ttcagaacgc tccgcgatgt ctcgaggttg aggcggccaa
     2221 actccctttc aaggtcgttc aagtcttgcg cggtgatgcg gtgatccttt gcaccaagaa
     2281 acagcaatga cattgtggtc tgccacgaaa agtcgagggc cttcgccagc accagcagca
     2341 tttctctgtt cttgtcggcc aaggcgcgct ctatcacgtc ggctggcaat gagcacaaca
     2401 aggacaggcc gatggtgacc tcttcgaacc tgtgggattg agcgtatccg gatatgctgt
     2461 tctcgttcag attgccctgc tgatgctgcg tcgtcacgag ccgttttgcg acaaagtagc
     2521 ttctggatac cggaccgaac ttggactgca gcgcgcccgc gacatcggtc accgaggtct
     2581 gaatttggtc gaccatcgca ggccgctcgc gctcgagccg ctttttcacg tcctccgatg
     2641 cctttgcgat caactgctgg aaaacccgcc tcggaatgtc cccgcggaga ccgagttgct
     2701 cggcgaggat ggaatcgcct tcggcgcgct tgaccatgtg caaaaagccg aagtctgaaa
     2761 ggcgtgcgcc ggcattactt gcaatcgaat tcacgacgtc ccgatcgccg cgcgccacca
     2821 gcacgtccgt gactgtttct ccgatggact ttcgttgtga gatggcgaga aggtgggact
     2881 ggcttttggt agtggcgttg gcaatcaggg cgttatcgtc gagctgctcg gactcccgga
     2941 gtacggggcc agcgacatcg atcgaatcgt caaaggcaag cttgtggatg atgttgatcg
     3001 gggcattgtc gaagttggcc agccgttttg caagctgcgc gcgggcggcg acttcaatct
     3061 catcagccaa ccgcccaatg acctcgccaa aggtccatat ctcatcgtcg ctataccggc
     3121 cggctatcaa cagatccgtc gcatgccaca gcgcgcgctt ccggctttcc gccgttccac
     3181 gcgcgactgc gtcgtcgaga tcgtctagaa acgattttac ctcagtcatc ttgacagacc
     3241 ttcaatcgca acaatcgcgg gcaagcaatt cgcctggttc aaccatagcc agcaaatgaa
     3301 actttttggt aaaggagggc ggcaactgca gccgccaaga aattcgctaa aatccgcaca
     3361 aacctgttcg ctttttggtc atgctaacct tcacaagccg caccccgatg aaaatcttcc
     3421 cccgaatgat tgcagttttg gcgctgtttc tttctgcgcc ggccgacgcg atcgttggag
     3481 gcggggtgcc gtccgccgag ggcgtcggcc gctccgccgt caccatcgtc ggctcccgcg
     3541 gtaatttctg caccggcgcc ttgattgcat ccagactggt gctgacggcg gcgcattgcg
     3601 tgcagcccgg cgcggtttac aagatcgtcg aatatggcgc agacaagcag ccgcagctgc
     3661 gagatgtcaa aaacgtcgcc attcaccccg cgttcaacat gcaggcgata atggcgcatc
     3721 gcgccaccgc cgatgtggcg ttgctgcagc tcgaggcgcc gccgaacggg aaaaatgccg
     3781 ccgatctcgg tgcaccgcag gtcccgattg tcgccggcag ccgtttcacc atcgccggca
     3841 tcggcgtcgc catccgtggt gagggcaaga gcggcgggac cgttcgcgtc gcgggcctgg
     3901 tcgcgaccgg ccaacccggg accctgcaaa tccgcctggt cgatccggtc ggacagggtg
     3961 cgcgggaggg gcttggcgcc tgtaccgggg attccggcgg cccggtgttt gaggaccggc
     4021 acggcgcccc ggccatcgtc ggcgtcatca gttggtcgac ggggccgaac ggcagcgcgg
     4081 gctgcggcgg cctgaccggc gtcacgccgc ttacgcttta tcgcgactgg atcgtgcaga
     4141 ccgcgcggca atggggtttt gccctgtgag gttggcggtc aatctggcgc atgctcaggc
     4201 gtcggcggat ctcgaggcca gctcagcacc atttctgccg acaatcgcgt tttgcagatt
     4261 gttgagctgt cgcgctgccg catcctgcat cagcggaacc gacagccggc aaaccagacc
     4321 ttcggaacgc cagtcgaact cggcttgtcc tccgagctgc gactcgatgc tggcgatcac
     4381 gctccttgtt ccgaatccgc gtgctaccgg tctctcgaca acagggccat ccttctcttc
     4441 ccataccatc cgcagcacgt cttcttgggt ctcccacttg atcgagagac gaccggacag
     4501 cgtggagagg gcgccatact tt
//
LOCUS       DATNOI010000660         3810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1487085,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000660 DATNOI010000000
VERSION     DATNOI010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487085"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..426)
                     /gene="gltD"
                     /locus_tag="VN968_26535"
                     /pseudo
     CDS             complement(247..426)
                     /gene="gltD"
                     /locus_tag="VN968_26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139023.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
     gene            complement(1029..2486)
                     /locus_tag="VN968_26540"
     CDS             complement(1029..2486)
                     /locus_tag="VN968_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HXL32825.1"
                     /translation="MGKVTGFLEIDRHDRKYTPVAERVKSYREFVVPLSEKDTRDQAA
                     RCMNCGIPYCHGTGSVAPGAPGCPVNNQIPDFNDLVYSGNWEEASRNLHSTNNFPEFT
                     GRICPAPCEASCTLNIDDNPVTIKTIECAIVDRAWDNGWVTPEIPTTRTGKKVAVIGS
                     GPAGLACAQQLARVGHEVHLFEKFAKAGGLLRYGIPDFKMEKHVIDRRVAQMEAEGVT
                     FHYGAHVGGASPGAIDPREMLNEYDALALTGGAEAARDLPIPGRDLAGIHFAMDFLPQ
                     QNRRVSNEPLGSAPDILAGGKHVVVIGGGDTGSDCIGTSFRQGALSVTQLEIMPAPPE
                     HENKGLSWPNWPLKMRTSSSQAEGAKREFAVLTQNFSGADGKVAKLHCVQVDDKFKPI
                     PGTEFELEAQLVLLAMGFVHPVQEGLLKTLGVEFDPRGNVRANTTDYKTSRPNVFSAG
                     DMRRGQSLVVWAIREGRLCARAIDGFLMGTTNLPR"
     gene            complement(2638..>3810)
                     /locus_tag="VN968_26545"
     CDS             complement(2638..>3810)
                     /locus_tag="VN968_26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HXL32826.1"
                     /translation="LRKRFTGQPEHVINYFFFVAEEVRGIMAALGYRTFNEMIGQTQM
                     LDQSKLVAHWKAKGLDFSKLFVRQKELPGQKIYHSQGQNHHLEAVLDRRLIEQSQAAI
                     DRGAPVRIEMAINNTDRSAGAMLSGTVAKIYGHAGLPHDTIHVGFKGTAGQAFGAWLA
                     RGITFDLEGEGNDYVGKGLSGGRIVVKPPRNSGIVPEESIIVGNTVMYGAIEGECYFR
                     GIAGERFAVRNSGAVAVVEGAGDHCCEYMTGGIVVVLGKTGRNFAAGMSGGVAYVLDE
                     AGDFEKLCNMAMVQLEPVLSEEMINANAYHQSGDLEAHGRVDVFKDLLESDVERLHIL
                     ITRHARLTGSQRAADILADWKTSLPKFRKVMPVEYRRALKELKANADAEPKIAIGA"
BASE COUNT          668 a         1201 c         1194 g          747 t
ORIGIN      
        1 gtcttctggt cgacgacgcg gcgcgcccga acatcatctc caatgtggcc gcgaactgct
       61 ggcaacccta tgtcaggaat tacatcccgc acgacaacag ccaatacaac agcctgcggt
      121 tcgaggacgt ctggctggac aagtagaggc gtcgtcatac gcgggctcga cccgcgtatc
      181 catcaatctt cgtagcagtc ttgcgaataa gatgggttgc cgggtcaagc ccggcaacga
      241 cggcagttac cgcggcaggt tggtcgtgcc catcaaaaac ccgtcgatgg ccctggcgca
      301 gaggcggcct tcgcggatcg cccggacgac gagcgactgg ccgcggcgca tgtcgccggc
      361 cgagaacact ttgggccgcg aggtctgaca gttggtgacg ttggcccgca cattgccggg
      421 cgggtcctag cgtttccagt tacttcggcg gatgtagttc gccggtgaac caaccaaccg
      481 catcggattc catctgatct aacgctatcg cgatctgttc gacgtgatcg catttggggc
      541 agtcgaatgt gcgaagttcg aatccggtag ggccgggcaa gatgtgcgta agcgtcatgc
      601 gcgttcggca ttgcgggcag tggaggtgct gaattgcgag gagtgggatt gcggctgggt
      661 agtcggtcat tgcattcccc ttggattggg cgggagcgca aaactctcag tcaccggtag
      721 aagccgaaga ggccagtgat gggatcattg gattccctat ccgtccggga gtcgacttga
      781 gaaacattaa ttcggcgagc ccagggagcg tttctccgat cggcaacttg gcgctgccag
      841 caccggtaaa atcgccgctt actgcgttgc gcaacaatgg tgtgataccc ggccatcggg
      901 tctcgccttc gggcgagccc gatgacaggc tccagcgggg tatccagtac gctgcagcct
      961 cgcgattctt cactggcgct ctggaatact gggccccccg ctttcgcggg gcgaagaggc
     1021 ggcggttttc atcgcggcag attggtcgta cccatcaaaa acccgtcgat cgccctggcg
     1081 cagaggcggc cttcgcggat cgcccagacg acgagcgact ggccgcggcg catgtcgccg
     1141 gccgagaaca cgttgggccg cgaggtcttg tagtcggtgg tgttggcgcg cacgttgccg
     1201 cgcgggtcga actcgacgcc gagcgtcttc aacaggccct cttgcaccgg atgcacaaaa
     1261 cccatcgcca gcagcaccag ttgcgcctcg agctcgaact cggtgccggg tatcggcttg
     1321 aacttgtcgt cgacctgcac gcaatgcagc ttcgcgacct tgccgtcggc gcccgagaag
     1381 ttttgcgtca gcaccgcgaa ttcgcgcttg gcgccttcgg cctggctcga cgaggtccgc
     1441 atcttcaagg gccagttcgg ccaggacagg cccttgtttt catgctcggg cggcgccggc
     1501 atgatttcaa gctgcgtcac cgacagcgcg ccctgccgga acgaggtgcc gatgcagtcc
     1561 gagccggtat cgccgccgcc gatcaccacc acatgcttgc cgccggccag aatgtcgggc
     1621 gcgctgccga gcggctcgtt actgacgcgg cgattttgtt gcggcaggaa atccatcgcg
     1681 aagtgaatgc ctgcgagatc gcggccggga atcggcagat cgcgcgcggc ctcggcgccg
     1741 ccggtcagcg ccagtgcgtc gtattcgttc agcatctcgc gcggatcgat cgcgccggga
     1801 gacgcgccgc cgacatgggc gccgtaatga aacgtgacgc cctcggcttc catctgcgcc
     1861 acgcggcggt cgatgacgtg cttctccatc ttgaaatcgg ggataccgta ccgcagcagg
     1921 ccgccggcct tggcgaattt ttcgaacaga tgcacctcgt ggccgacacg cgccagttgc
     1981 tgcgcacagg caagtcctgc gggacccgaa ccaatcaccg cgaccttctt gccggtcctg
     2041 gtggtcggga tttctggcgt gacccagcca ttgtcccagg cgcgatcgac gatggcgcat
     2101 tcgatggttt tgatggtgac cgggttgtcg tcgatgttga gcgtgcagga ggcctcgcac
     2161 ggcgccgggc agatgcggcc ggtgaattcc ggaaagttgt tggtcgagtg cagattgcgc
     2221 gaggcctctt cccagttgcc agaatagacc agatcgttga aatccggaat ctggttattg
     2281 accgggcagc ccggcgcgcc cggggccacc gagcccgtgc cgtggcaata gggaatgccg
     2341 caattcatgc agcgcgcggc ctggtcgcgg gtgtccttct cgctcagggg aaccacgaat
     2401 tcgcgatagc ttttcacgcg ctcggcgacc ggcgtgtatt tccgatcgtg acggtcgatc
     2461 tccagaaaac ccgtaacctt gcccattaaa gccagcgccc ctgcatctct acctcgtcgc
     2521 cgtcatcctg aggagcggca tcttcgctgc gtctcgaagg atgagacaca aacactctcg
     2581 cttgtcttcc ttcgaggctc gccaaagacg gctcgcatct cctgatgacg gttcctgcta
     2641 agccccgatc gcgattttcg gctcggcgtc ggcgttggcc ttcaattcct tcagcgcgcg
     2701 gcggtattcc accggcatca ccttgcggaa tttcggcaag gaagttttcc agtccgccag
     2761 aatgtcggcg gcgcgctggg agcccgtcag cctggcgtgg cgcgtgatca ggatatgcag
     2821 ccgctcgaca tcggactcca gcaggtcctt gaacacgtcg acccggccat gcgcctcgag
     2881 atcgccggac tgatgatagg cgttcgcgtt gatcatctct tccgacagca ccggctcgag
     2941 ctgcaccatc gccatgttgc agagcttctc gaagtcgccg gcctcgtcca gcacataggc
     3001 cacaccgccg gacatgccgg ccgcaaaatt gcgcccggtc ttgccgagca ccacgacaat
     3061 gccgccggtc atgtattcgc agcaatggtc gccggcgcct tcgaccacgg ccacggcgcc
     3121 ggaattgcgc accgcaaacc gttcgccggc gatgccgcgg aaatagcact cgccctcgat
     3181 cgcgccgtac atcacggtgt tgccgacgat gatggattct tccggcacga tgccggaatt
     3241 gcgcggcggc ttgacgacga tgcggccgcc ggacaggccc ttgccgacat agtcgttgcc
     3301 ttcgccttcg agatcgaagg tgatgccgcg cgccaaccag gcgccgaacg cctggcccgc
     3361 ggtgcccttg aagcccacgt gaatggtgtc gtgcggcagc ccggcatgac cgtagatctt
     3421 tgcaaccgtt cccgacagca tcgcgccggc gctgcggtcg gtgttgttga tcgccatctc
     3481 gatcctgacc ggcgcgccgc ggtcgatcgc ggcctgcgac tgttcgatca gcctgcggtc
     3541 gagcacggcc tccagatgat ggttttggcc ttgcgaatga tagattttct ggcccggcag
     3601 ttccttctgg cggacgaaca gctttgagaa atccagcccc ttggccttcc agtgcgccac
     3661 cagcttggac tggtcgagca tctgggtctg gccgatcatc tcgttgaaag tgcggtagcc
     3721 cagcgcggcc atgatcccgc gcacttcctc ggcgacgaag aagaagtaat tgatgacgtg
     3781 ctcgggctgg cccgtaaaac gcttgcgcag
//
LOCUS       DATNOI010000661         8480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1074016,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000661 DATNOI010000000
VERSION     DATNOI010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8480
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074016"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /gene="fba"
                     /locus_tag="VN968_26550"
     CDS             complement(<1..840)
                     /gene="fba"
                     /locus_tag="VN968_26550"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799936.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HXL32827.1"
                     /translation="MARITLRQLLDHAAEHDYGVPAFNINNMEQALAIMDAASSLDAP
                     VIIQASRGARSYANDIMLKHMMDAVTEIYPQIPVCVHLDHGNGPDTCMTAIQAGFTSV
                     MMDGSLKPDGKSPADWDYNVGVTRTVTDMAHLGGISVEGELGVLGSLETGMGDKEDGH
                     GAEGKLSHDQLLTNPDEAVKFVKETKVDALAIAMGTSHGAYKFTRKPDGDILAMNVIE
                     EIHRKLPNMHLVMHGSSSVPQDLQDIINANGGKMKPTWGVPVSEIQRGIKNGVRKINI
                     DTDN"
     gene            complement(908..1231)
                     /locus_tag="VN968_26555"
     CDS             complement(908..1231)
                     /locus_tag="VN968_26555"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HXL32828.1"
                     /translation="MKRGDLVTVILPGAHGKPRPAVIVQSDRYEAMESITFLPMTSAV
                     LPKQVFRIAVVPTAQNGLRTPSQIMADKCSTLPLARVGSVFGRLSTADLGRVDRALAA
                     FLGFA"
     gene            complement(1228..1434)
                     /locus_tag="VN968_26560"
     CDS             complement(1228..1434)
                     /locus_tag="VN968_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE family protein"
                     /protein_id="HXL32829.1"
                     /translation="MADGAKRVQKHRDKMKAAGLKPVTIWVPDVNAPGYAQQVAEAIA
                     IINASADEKIVLEELSNIEIEGWK"
     gene            complement(1503..2714)
                     /locus_tag="VN968_26565"
     CDS             complement(1503..2714)
                     /locus_tag="VN968_26565"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HXL32830.1"
                     /translation="MSKTFCTLDDVDVKGKRVLLRVDLNVPMENGRVSDATRLERVAP
                     TITEISDKGGRAILLAHFGRPKGRDAKDSLKPVAAALAQVINKPVAFADDCVGEAAQE
                     AIAAMKDGDILCLENTRFHKEEENNDPAFVAELAKLGDIWVNDAFSAAHRAHASTEGL
                     GHKLPAYAGRTMQAELDALGKALDAPTKPVIAIIGGAKVSTKIDLLENLVTKVDALVI
                     GGGMANTFLHAQGVGVGKSLAEKDLAATALRIVEKAEAANCAIILPVDAIVAFHFAAN
                     APSHAYGLDAIPPDGMILDVGPQSIARIHAAIDDAATLVWNGPLGAFEMTPFDRGTMV
                     AARHAAERTKARKLISVAGGGDTVAALNQAGVAGDFSYVSTAGGAFLEWMEGKPLPGV
                     EVLKAEVLKAK"
     gene            complement(2863..3870)
                     /gene="gap"
                     /locus_tag="VN968_26570"
     CDS             complement(2863..3870)
                     /gene="gap"
                     /locus_tag="VN968_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HXL32831.1"
                     /translation="MAVRVAINGFGRIGRNILRAIAESGRKDIEVVGINDLGPVETNA
                     HLLRFDSVHGRFPGTVTVDGDTISLGNGKIKVTAVRDPNTLPWKDLGIDIALECTGIF
                     TAKDKASAHLTAGAKRVLVSAPADGADATIVFGVNHDKLNKDHLVVSNGSCTTNCLAP
                     VAKVLHDTVGIETGFMTTIHAYTGDQPTLDTLHKDLYRGRAAAMSMIPTSTGAAKAIG
                     LVMPELAGKLDGVSIRVPTPNVSVIDLKIIAQRKTDVKEINEAMKRASEQQLKGILGY
                     TTAPNVSIDFNHDPHSATFHMDQTKVQNGTLVRVMAWYDNEWGFSNRMADTAVAMGKL
                     L"
     gene            complement(4016..6001)
                     /gene="tkt"
                     /locus_tag="VN968_26575"
     CDS             complement(4016..6001)
                     /gene="tkt"
                     /locus_tag="VN968_26575"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXL32832.1"
                     /translation="MTHVDPSRMANAIRGLAMDAVEKAKSGHPGLPMGAADIATVLFT
                     QFLKFDAADPKWPDRDRFILSAGHGSMLLYSLLYLTGNTEMTLDQLKHFRQLGSLTPG
                     HPENFHTKGIETTTGPLGQGIATSVGFALAEKMLAAEYGKKVVDHHTYVLASDGDLME
                     GVSQEAIALAGHWKLDKLIVLYDDNGISIDGPTSLSDSVDQVKRFKSAGWQAELIDGQ
                     DQQAIAAAITRAQKSNKPSMIACKTTIGFGAPTKAGTAKAHGEALGAEELKGAKEKLG
                     ISLEPFSVPDDVLKSWREAGSRGAAARKEWEARFAELTARRRTEFERRLRHERPAALA
                     KAFKAHKKALLETPQNIATRKSSESVIDAIVTAMPEFVAGSADLTGSNNNKAKSAIDF
                     SAKTPKGRFIHYGVREHGMAAAMNGIFLHGGFAPNGATFLVFTDYARPAMRLAALMGT
                     GVVYVMTHDSIGLGEDGPTHQPVEHLAALRAIPNMRVFRPCDAVEVAECWELALNRID
                     GPTVLALTRQNLPQLRTDAPADSPCSHGAYQLVAPQQGEARVSLFASGSEVEIAVAAQ
                     KQLGERGIASRVVSVPSLELLLAQPVEKRKAIIGNAPVKVAIEAAVRFGWDAVIGPDG
                     AFVGMHGFGASAPVKDLYQHFGITAEAAVNAALKLLG"
     gene            6281..6601
                     /locus_tag="VN968_26580"
     CDS             6281..6601
                     /locus_tag="VN968_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 domain-containing protein"
                     /protein_id="HXL32833.1"
                     /translation="MNDHSTNGSGSAEAVSVDIDAASRRLMTALDALESAVERRREAD
                     RDENELATRIQALGADRSRLADELDGSLVKSRKLERTNREIAERLDAAIGTIRSVLDA
                     GETS"
     gene            6598..6981
                     /locus_tag="VN968_26585"
     CDS             6598..6981
                     /locus_tag="VN968_26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HXL32834.1"
                     /translation="MNHINVTINGRQYRMACEEGQEVQLLRLAESLESRIGTLRGKFG
                     EIGDARLIVMAALTVCDELLDAGQRIRGLEQELAALRNVRSVATERARATQAAVANAL
                     NSAADRIEKTTQVLNRTIGGGVAIG"
     gene            7036..7196
                     /gene="ssrS"
                     /locus_tag="VN968_26590"
     ncRNA           7036..7196
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VN968_26590"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7341..7913
                     /locus_tag="VN968_26595"
     CDS             7341..7913
                     /locus_tag="VN968_26595"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HXL32835.1"
                     /translation="MEAQKANLRAAALARRDALSPEQRAAAATAVAGRGLPFPISRGA
                     VVSGYSPIRSEIDPIPLMRTLAAQGAQLALPSITARGQSLAFRAWSANDRLLLGPLGI
                     LEPSSAAAEIIPEIMLVPLAAFDRAGHRIGYGAGHYDYTLAHLRKLKPIIAVGVAFAA
                     QEIEAIPALPHDVALDYVLTETRTFEFRSL"
     gene            7915..>8480
                     /locus_tag="VN968_26600"
     CDS             7915..>8480
                     /locus_tag="VN968_26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HXL32836.1"
                     /translation="MRILFVGDVVGRAGRTAISDYLPGMIRDWALDLVVVNGENAAGG
                     FGITEVIYQEFIDAGADAVTLGNHAWDQREALVFIERAPRLVRPANFPKGTPGRGTAL
                     VDTKSGKRALVLNAIGRVFMTPFDDPFAVINRELEACPLRAAADAIVVDFHCEATSEK
                     QGVGFFCDGRASLVVGTHTHVPTADHQIL"
BASE COUNT         1435 a         2668 c         2714 g         1663 t
ORIGIN      
        1 gttgtcggtg tcgatgttga tcttgcggac gccgttcttg atgccgcgct ggatctcgga
       61 aaccggcacg ccccaggtcg gcttcatctt gccgccattg gcgttgatga tgtcctgcag
      121 gtcctgcggc accgacgacg aaccatgcat caccagatgc atgttcggca gcttgcggtg
      181 gatttcctcg atcacgttca tggccagaat gtcgccgtcc ggcttgcggg tgaatttgta
      241 ggcgccgtgc gaggtgccca tcgcgatcgc cagcgcgtcg accttggttt ccttgacgaa
      301 cttcacggct tcgtcgggat tggtgagcaa ctggtcgtgg gagagcttgc cttccgcgcc
      361 gtggccatcc tccttgtcgc ccatcccggt ctcgagcgaa cccagcacgc cgagttcgcc
      421 ctccaccgaa atgccgccga gatgggccat gtcggtgacg gtcctggtga cgccgacatt
      481 gtagtcccag tcggccgggc tcttgccgtc gggcttcagc gagccgtcca tcatcacgga
      541 cgtgaagccg gcctggatcg cggtcatgca ggtgtcgggc ccgttgccgt gatcgagatg
      601 cacgcagacc ggaatctgcg gatagatttc ggtgaccgca tccatcatgt gcttcagcat
      661 gatgtcgttg gcgtaggacc gcgcgccgcg cgaggcctgg atgatgaccg gagcatcgag
      721 gctggaagcg gcgtccatga tggcgagcgc ctgctccatg ttgttgatgt tgaaggccgg
      781 cacgccgtag tcgtgctccg ccgcgtggtc gagcaattga cgcaacgtga tccgagccat
      841 gtgtcctgtt ctccgcgttg gggtgacagc aggctgtcac agtttcaaaa tacaacgtag
      901 ccgcttctca agcgaaaccc aagaaagctg cgagtgctcg atcgaccctt ccgagatcgg
      961 cagtgctgag acggccgaat acgctgccca ccctggccag cggcaatgta ctgcacttgt
     1021 ctgccataat ctgggacggc gtccgcaagc cattctgcgc cgtcgggacg accgcgatgc
     1081 gaaacacctg tttcggaaga actgcgctcg tcattggcag gaacgtaatg gattccatcg
     1141 cctcataccg gtctgactga acgatcactg caggccgagg ttttccatgg gcgccgggaa
     1201 ggataacggt gaccaggtca ccgcgtttca tttccagcct tcgatctcaa tatttgaaag
     1261 ttcttcgagg acgatctttt cgtccgcgct ggcgttgata atcgcgatcg cttctgcaac
     1321 ctgttgcgca tatccgggcg cattcacatc gggaacccat atcgtcaccg gcttgaggcc
     1381 ggccgccttc atcttgtccc ggtgcttttg aacccgcttg gctccgtcgg ccatggcata
     1441 catcctggaa ttgaagcgtt acatgtaaca taggccgggg ggcggcccag cttcaagttg
     1501 cgtcacttgg ctttgagaac ctcggctttg agaacctcga caccgggcaa cggtttgccc
     1561 tccatccatt caagaaacgc gccgcccgcg gtcgaaacat agctgaaatc gccggccacc
     1621 ccggcctgat tcaatgccgc caccgtgtcg ccgccgccgg caaccgagat aagcttcctg
     1681 gccttggtcc gttccgccgc gtgcctcgcc gccaccatgg tgccgcggtc gaacggcgtc
     1741 atctcgaacg ccccgagcgg accgttccag accagcgtcg cggcgtcgtc gatcgcggca
     1801 tgaatccgcg caatcgactg cgggccgacg tcgaggatca tgccgtcggg ggggatcgcg
     1861 tcgaggccgt aggcatggga cggcgcattg gctgcgaaat gaaacgcgac gatcgcatcg
     1921 accggcaaga tgatggcgca attcgcagct tcggccttct cgacgatccg cagcgcggtc
     1981 gccgcgagat ccttctccgc cagcgatttg ccgacaccga cgccctgggc gtgcaggaac
     2041 gtattggcca tgccgccgcc gatcaccagc gcatcgacct tggtcacgag attttccaga
     2101 agatcgatct tggtggaaac cttggcgccg ccgatgatcg caatgacggg tttggtcggc
     2161 gcgtccagtg ccttgccgag cgcgtcgagt tcggcctgca tggtgcgtcc ggcataggcc
     2221 ggcagcttgt ggccgaggcc ctcggtggac gcgtgcgccc ggtgcgccgc cgagaacgcg
     2281 tcgttgaccc agatgtcgcc gagctttgcc agttcggcga cgaacgccgg atcgttgttt
     2341 tcttcttcct tgtggaagcg ggtattttcc agacaaagga tgtcgccgtc cttcatcgcc
     2401 gcgatcgcct cttgcgcggc ttcgccgacg caatcgtcgg cgaacgccac cggcttgttg
     2461 atgacttggg ccaatgccgc cgcgacaggt ttcagggaat ccttcgcgtc gcggcccttg
     2521 ggacggccga aatgcgccag caggatcgct cttcccccct tgtcggaaat ttcggtgatg
     2581 gtgggtgcga cacgttcgag ccgggtcgcg tcgctgacgc ggccattctc catcggcacg
     2641 ttgagatcga cgcgcagcag cacgcgcttg cccttcacgt cgacgtcatc gagggtgcag
     2701 aatgttttgg acatctctag ctcttgtcct gggcgctgcg cgaagcggtg cgcagcagaa
     2761 ccgggaccca tgttggtgcg aggtcccggc tctgcggagc agcgtactgg acgatgccac
     2821 gcatcgccag gagtacgctg caccgcgtcc gggacgcgag acttacagca gcttccccat
     2881 cgccacggcg gtgtcggcca tgcggttgga aaagccccat tcattgtcgt accacgccat
     2941 cacgcgcacc agcgtgccgt tctgcacctt ggtctggtcc atgtggaagg tggcggagtg
     3001 cggatcgtgg ttgaaatcga tcgagacgtt gggtgcggtg gtatagccca aaatgccctt
     3061 gagctgctgc tcggaagcgc gcttcatcgc ctcgttgatt tccttcacgt cggtcttgcg
     3121 ttgcgcgatg atcttgagat cgatcaccga aacgttcggc gtcgggacgc ggatcgatac
     3181 gccgtcaagt ttgccggcaa gctccggcat cacgaggccg atcgccttgg cggccccggt
     3241 ggaggtcggg atcatcgaca tcgccgctgc ccggccgcga tagagatcct tgtgcagggt
     3301 atcgagcgtc ggctgatcgc cggtataggc atggatcgtg gtcatgaagc cggtttcgat
     3361 gccgacggtg tcgtgcagca cttttgcgac cggcgcgagg cagttggtcg tgcacgaacc
     3421 attggagacg accaggtgat ccttgttcag cttgtcgtgg ttgaccccga acacgatggt
     3481 cgcatcggcg ccatccgcgg gtgccgagac cagcacccgc ttggcgccgg cggtcagatg
     3541 cgcggaggcc ttgtccttgg cggtgaagat gccggtgcat tccagcgcga tgtcgatgcc
     3601 gagatccttc cacggcaagg tgttgggatc gcgcaccgcg gtcaccttga tcttgccgtt
     3661 gccgaggctg atcgtatcgc cgtccacggt cacggtgccg gggaagcggc catgcacgga
     3721 atcgaagcgc aagagatggg cgttggtctc gaccgggccg agatcgttga tgccgaccac
     3781 ctcgatatcc ttgcggccgg actcggcgat ggcccgcagt atgttgcggc cgatgcggcc
     3841 gaacccgttg atcgcgaccc ggactgccat ctgtctctcc ttcaattatt cttgctaccc
     3901 ccgcggaagg cgcttgagcc gtccacgcgg gtttttgcgg cggacgcccg gttcggccgg
     3961 gcgggagcgg ataatatgcc cgttaggtag tccttttttc ggccaatctc aaccatcagc
     4021 ccaatagctt cagcgcagcg ttaaccgccg cctcggcggt aattccgaaa tgctggtaaa
     4081 ggtccttgac cggcgcgctg gcgccgaaac cgtgcatgcc tacaaatgcg ccatccggcc
     4141 cgatcaccgc atcccagccg aaccggaccg cggcctcgat cgcgaccttg acgggggcgt
     4201 taccgataat ggccttgcgt ttctccaccg gttgcgccag cagcaattcc agcgagggca
     4261 ccgagaccac ccgcgacgcg atgccgcgct cgccgagttg cttctgcgcg gcgaccgcga
     4321 tctcgacttc ggagccggag gcgaacagcg ataccctggc ctcgccctgc tgcggtgcga
     4381 ccagttgata ggccccgtgg ctgcaggggc tatcggcggg cgcatcggtc cgcaattgcg
     4441 gcaggttctg gcgggtcagc gccagcaccg tcggaccatc gatccggttg agcgccagct
     4501 cccagcattc ggctacctcg accgcgtcgc agggacggaa cacgcgcatg ttcggaatgg
     4561 cgcgaagggc cgcgagatgt tcgaccggct gatgcgtcgg tccgtcttcg ccgaggccga
     4621 tcgaatcatg ggtcatcaca tagacgacgc cggtgcccat caacgccgcg agccgcatcg
     4681 ccggccgggc ataatcggtg aagaccagga aagtcgcgcc attcggcgcg aatccgccgt
     4741 gcaaaaagat tccattcatg gccgccgcca tgccgtgctc gcggacgccg taatggatga
     4801 aacggccctt cggcgtcttg gccgaaaagt cgatcgccga tttcgccttg ttgttgttgg
     4861 agccggtgag atcggccgaa cccgccacga attccggcat cgccgtcacg atcgcgtcga
     4921 tgacggattc ggaggatttg cgggttgcga tattctgcgg tgtttcaagc agcgccttct
     4981 tgtgggcctt gaaggctttc gcaagggctg ccggccgctc atgccgcagg cgacgttcga
     5041 actcggtgcg ccggcgggcg gtcagttcgg caaagcgagc ctcccactcc ttgcgcgccg
     5101 cggcgccgcg gctgccggct tcgcgccagc ttttcagcac gtcgtcgggc accgagaacg
     5161 gctccagcga aatgccgagt ttctccttgg cgcccttgag ctcttcggcg cccagtgcct
     5221 cgccatgcgc cttggcggtg ccggccttgg tcggcgcccc gaaaccgatg gtggtcttgc
     5281 aggcgatcat cgaaggcttg ttggatttct gcgcccgcgt gatcgcggcg gcgatggctt
     5341 gctgatcctg gccgtcgatg agttcagcct gccagcccgc cgacttgaac cgttttacct
     5401 ggtcgaccga gtcggagagc gaggttggac cgtcgatcga aatgccgttg tcgtcgtaca
     5461 gcacgatcag cttgtcgagc ttccagtgcc cggcgagcgc gatcgcttcc tgcgacacgc
     5521 cttccatcag gtcgccgtcg gacgcgagca cataggtatg atggtcgaca accttcttgc
     5581 cgtactcggc ggcgagcatc ttctccgcga gcgcgaagcc gaccgacgtc gcgatgccct
     5641 ggcccagcgg gccggtcgtg gtttcgatgc ccttggtgtg gaaattttcc gggtggcccg
     5701 gcgtcagcga tcccagctgg cggaagtgct tgagctggtc gagcgtcatc tctgtattgc
     5761 cggtcaggta caacagcgaa tacagcagca tcgaaccatg cccggccgac aggatgaatc
     5821 ggtcgcggtc cggccatttc gggtcggcgg cgtcgaattt caggaactgg gtgaacagca
     5881 cggtggcgat gtcggccgca cccatcggca ggccgggatg gccggatttc gccttctcga
     5941 cggcatccat cgccagcccg cgaatcgcat tggccatacg ggagggatcg acatgcgtca
     6001 tgatgaaaat ccgcctgaaa tgagctgccg tggaggtgat tcgcgggcgg cggcgaatgg
     6061 ccaggcagga ctgtggtaac gggaggggct ggatagcatc tcggttcccg gagtccaagg
     6121 cgggcaaacg cgccaagggg ccgaaaaatt gctttgcggt gcatagctgc gaagcgccgt
     6181 tgcgctggcc tcgcttgcca cgtaaatttc ccggtctaaa cgcttgccga accgcgaatt
     6241 ttaccgtgcg gcgggctcat ggaaggttcg cgctgtcagc atgaacgatc attccaccaa
     6301 cggttccgga agcgccgagg cagtatcggt cgatatcgac gctgcctcgc ggcggctgat
     6361 gacagcgctc gatgcactgg agagcgcggt cgagcggcgt cgcgaagccg atcgcgacga
     6421 gaatgagctg gcgacccgga ttcaggcgct gggcgcggat cgatcgcggc tggccgacga
     6481 actcgatggc tcgctggtga aatcgcgcaa gctggagcgc accaatcgcg agatcgcaga
     6541 acggctggat gccgcgatcg gcaccatccg ttcggtactc gatgccggag agacttcatg
     6601 aaccatatca acgttaccat caacggccgg caataccgga tggcgtgcga ggaaggccag
     6661 gaggtgcagc tgttgcggct cgccgaaagc ctcgaatcgc ggatcggtac gctgcgcggc
     6721 aagttcggcg agatcggcga tgcgcgcctg atcgtgatgg ctgcgctgac ggtgtgcgat
     6781 gaattgctcg atgccggtca gcgcatccgc ggcctcgaac aggagctcgc ggcgttgcgc
     6841 aacgtccgct cggtggcgac ggaacgcgcc cgggccacgc aggccgcggt cgccaatgcg
     6901 ctgaactccg ccgccgaccg catcgagaag accacgcagg tgctcaaccg caccatcggc
     6961 ggcggcgtgg cgattggttg accatcgcct gcgttaacgc tggcggatcg gttccatcgt
     7021 ggttacagta atgacgcgaa gctgtgtcgc gcgtcaggag ccatatatcc ccggggcctt
     7081 atcgatcctt ccgggaactg tccctggcca ggtccgtgga cttggtcaca tggtgcccac
     7141 ctactttcgt agggaactcc gggatcgagc gctccaacgg ccattacggc ttcgcacttt
     7201 tcttgctaga tatgaccttc catcgtcatg gccgggcttg tcccggccat ccacgtcttt
     7261 agcatgcgtg cgaagaaaga cgtggatgcc cggcacaagg ccgggcatga cggtattcaa
     7321 tgccgagcct ctctgcttcc ctggaagcac aaaaagcaaa tctccgcgct gccgcgcttg
     7381 caaggcgcga tgcgttgagc cctgagcagc gcgccgccgc cgcgacggcc gtcgccgggc
     7441 gcggactgcc gtttccgatc agccgcggcg ccgtcgtctc cggctattcg ccgatccgca
     7501 gcgagatcga tccgatcccg ctgatgcgga cgctcgcagc ccagggggcg cagttggcgc
     7561 tgccgtcgat caccgcgcgc ggtcagtcgc tcgcattccg ggcctggtct gccaacgaca
     7621 gattgctgct cggaccgctc ggcatccttg agccgtcgtc cgcggccgcc gaaatcattc
     7681 cggaaatcat gctggtgccg ctggcggcgt tcgaccgcgc cggccaccgc atcggctatg
     7741 gtgccgggca ttatgactac accctggcgc atctgcgcaa attgaaaccc atcatcgccg
     7801 tcggcgtcgc gttcgcggcg caggaaatcg aggccattcc ggcgctgccg cacgacgtgg
     7861 cgctggatta tgtgctaacg gaaacgcgga cattcgaatt ccggagcttg taacttgcgc
     7921 attcttttcg tcggcgacgt ggtcggccgg gccggccgta ccgcgatctc agactatttg
     7981 cccggcatga tccgcgactg ggcgctcgat ctcgtggtcg tcaatggcga gaatgccgcc
     8041 ggcggtttcg gcatcaccga ggtgatctac caggaattca tcgacgccgg cgccgacgcg
     8101 gtgacgctcg gcaatcatgc ctgggatcag cgcgaggcgc tggtgttcat cgagcgcgcg
     8161 ccgcgcctgg ttcgcccggc gaattttccc aagggcacgc cgggccgcgg caccgcgctg
     8221 gtcgacacca agagcggcaa gcgcgcgctg gtcctcaacg ccatcggccg cgtcttcatg
     8281 acgccgttcg acgacccgtt cgccgtcatc aaccgcgagc tcgaagcctg tccgttacgc
     8341 gcggcggcgg atgccatcgt ggtggatttc cattgcgagg cgaccagcga aaagcagggc
     8401 gtcggatttt tctgcgacgg ccgcgccagc ctcgtggtcg gcacccatac ccatgtgccg
     8461 accgccgacc atcagatcct
//
LOCUS       DATNOI010000662         6424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2225223,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000662 DATNOI010000000
VERSION     DATNOI010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2225223"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VN968_26605"
     CDS             complement(<1..366)
                     /locus_tag="VN968_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HXL32837.1"
                     /translation="MRRNQVAVVGAAETTELGVIPNVSQIQLHADAALNAIADAGLKL
                     SDIDGFATAVETPQQIAHYLGITPTWVDGTSVGGCSFMLHVRHAAAAIEAGLCKTVLI
                     THAESGKSNIGRTPRMIGPD"
     gene            complement(372..782)
                     /locus_tag="VN968_26610"
     CDS             complement(372..782)
                     /locus_tag="VN968_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HXL32838.1"
                     /translation="MAEPARAQPKPTPETQHFWDGTQAGELRLQRCDACANVYFPPRP
                     FCPACGCRKVSMFKASGKAKLYSYVIHHRPVPGFTPPYAIAVVELDEGPHMMSNIVDC
                     PQTPEALELDMKLEVAFEKLDDTITLPLFRPAKG"
     gene            991..2238
                     /locus_tag="VN968_26615"
     CDS             991..2238
                     /locus_tag="VN968_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL32839.1"
                     /translation="MLKVTGAVLATGLTFAAPAFAADAPAEIKIGTLYASSGRYASIS
                     MPVHSALKLWIDQKNADGGVYVKAFDKKIPVKLVAYDDQSNTATASTLYNQLITQDKV
                     DLLVADSGSVLTAPAVAIARDRKMFLFDQTGTGASFFSKDNPYIALMADPVSTIWPKP
                     VADFVSHDGPGLGIKKIAILYSTNEFTGTQANAFRKFVKDSGAPIEIVYDQGIPTETT
                     NYTVIINNINNTQPDAVIHFGYAPNDIAFLRNVQDVGVKFKMLFSIYAGLETELLEKN
                     VGEKGLEHVFTYVPPSELEYPVNFGMTMKDYKAAWDKKYPDGKIEFGFNAVAGYTTGL
                     VIEKTLSVATSLDQLELRRAVFSLSGELKTLDGTFALDETGGQIGELTPLGQLALDEH
                     EHIKFISIYPHETATGKPVYPRP"
     gene            2282..3208
                     /locus_tag="VN968_26620"
     CDS             2282..3208
                     /locus_tag="VN968_26620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL32840.1"
                     /translation="MLTYALVAGVLFGLYFSLVGIGLNLVFGVMRIVNLAHGDFLMLG
                     AFVAFGVVTLAGIDPLFAVPLAFVIFVVIGLPLYWVLVPRLQGSVNPEMLSIILFFGL
                     SQVIEAVTTIFFGTSERSIQSRALGTVFSTIRIKLFGGRPDAAGPIQIFGQGFPAAWV
                     IAAITSLIAISLVYVYLYRTRLGTLTRAVMSRRDEAFATGIDVDRVSAAAFGIGLGLA
                     AVAGVFAPFMFGSVTPALGADATVTSFAIVVLGSLGNPLGTALGGVVYGVCYMLVQTY
                     LSSWADLLPYVLLIFILLLRPSGLLGRQVRVA"
     gene            3201..4121
                     /locus_tag="VN968_26625"
     CDS             3201..4121
                     /locus_tag="VN968_26625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL32841.1"
                     /translation="MPSAFRHVLFIVVPLLAVFAVLPGVYQNHLLLFNFVIFLILAQG
                     VNIIYGFTGYLPFGYVGFFGAGAYGFAIMVMHFQTPAIVAVLVAGIVGVALGLLLTPL
                     LRLSGAYFAIANLAASLAVLHFVANPALENITRGPYGVSLTGTFNPTLAYAAALVVLA
                     LTLSAVVFLKNSRFGLALQAVREDAVSASMAGVDVVRMRVIAWLASALVAGLAGGVYA
                     WYVSVFYPDNVFSGEFSIFAIVFALFGGVATITGPIVGVIILYGIYNLIGFTTPQYFQ
                     LIYGLLIMGLVLFLPAGLVSLATRRGWHVP"
     gene            4111..4842
                     /locus_tag="VN968_26630"
     CDS             4111..4842
                     /locus_tag="VN968_26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32842.1"
                     /translation="MSLDRAPILNVTKLTKRFGGFYALDGLSFHVASGEILGLVGPNG
                     SGKTTAINVISGLYAPDGGEVVLDNADIGGIASHRLVHRGINRTFQIPKPFLSLTVRE
                     NISVALAYGRAAVAPPAMAELLDEYRLNEVADRPAADLNNAQQKMLDLVRALATRPRL
                     LLLDELAAGLNPAELDWIAERIKALARSGIAIIVVEHLMGFIEHITDRVIVMNAGKEI
                     FEGKLAVAVKVPQVIEVFLGGEHAA"
     gene            4832..5551
                     /locus_tag="VN968_26635"
     CDS             4832..5551
                     /locus_tag="VN968_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32843.1"
                     /translation="MPPDAAPLMEAKGVDAGYGTMQVLWGVDLDVRAGETVLLLGANG
                     AGKTTFLKSLVGLIEARHGSIQLAGEDVTRMRSSDRMRLGMTYMSELAVFPDLSIEEN
                     IRVGAQALGHANPGARAEELYGVFPALREKRRAPASSLSGGQRKMLGIAKALAAEPRL
                     LVMDEPSAGLSPLFVKEVIRILSDLRGRGLALLIAEQNIGFLEVATRVFVLEGGRIRF
                     SGTVAEMTDNEALRRAYFGLK"
     gene            5551..>6424
                     /locus_tag="VN968_26640"
     CDS             5551..>6424
                     /locus_tag="VN968_26640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HXL32844.1"
                     /translation="MRTIRIGSGAGYSGDRIEPAIELAEKGDIQYLVFECLGERTVAL
                     AQQARMKNPDGGYDPLLEERMRAVLPICAAKGIKIVTNMGAAHSVAAARKTAEIARSL
                     GLASLKIAAVIGDDVLDACKARDLPIMEFDGTIKLLGNRLLSANAYLGAEPIADALTG
                     GADIVITGRASDPALFLAPMIHAFGWAMDDWNLLGQGTVAGHLLECAGQITGGYFADP
                     GYKDVLDLARLGFPIGEVGEDGSLVITKVTGSGGAVTAQTCKEQLLYEVHDPTRYLQP
                     DVTADFSQVKVEEIG"
BASE COUNT         1091 a         1944 c         2025 g         1364 t
ORIGIN      
        1 gtcggggccg atcatgcgcg gcgtgcggcc gatattcgac ttgccgcttt cggcatgggt
       61 aatcagcacg gtcttgcata gcccagcctc gatcgccgcc gcggcgtggc ggacatgcag
      121 catgaacgag cagccgccga ccgatgtgcc gtcgacccag gtcggtgtga tcccgagata
      181 atgcgcgatc tgctgcggtg tttcgaccgc ggtggcaaaa ccgtcgatgt cggacagttt
      241 taggccggcg tcggcgatgg cgttgagggc ggcgtcggcg tgcaactgga tctgcgacac
      301 atttggaata acgccgagtt cggtggtttc ggcagcgcca acgacggcga cctgatttct
      361 gcgcatggtg tctagccctt cgccggacgg aacaagggaa gggtgatggt gtcgtcgagc
      421 ttctcgaacg cgacttccag cttcatgtcg agttcgagcg cctccggcgt ctgcggacaa
      481 tcgacgatgt tgctcatcat gtgcggacct tcgtcgagct ccaccacggc gatggcgtaa
      541 ggcggcgtga agccgggtac cgggcggtga tggatgacgt agctgtagag tttcgccttg
      601 ccgctggcct tgaacatgct gaccttgcgg cagccgcagg ccgggcagaa cgggcgcggc
      661 gggaaataga cgttggcgca ggcgtcgcag cgctgcaggc gcagttcgcc cgcctgtgtg
      721 ccatcccaga aatgctgggt ttccggtgtt ggtttcggtt gtgcgcgcgc tggctcggcc
      781 atgcctgtga ttcctccctg ggccggtcca cgtccgccca ccggctccga tcttgatgcg
      841 acattggacg gtcctgcgtc aacggtccag ccggcctgcc gcatgccgcg tattccggcc
      901 ggcgcactat tgcttggccc ggagcgctgg tgtacctccg accccgcgcg gtagattgtc
      961 cgtcagagcg aagaaaatcg ggaggattcc atgttgaagg tgacaggcgc ggtattggcg
     1021 acgggtttga cattcgccgc accggctttt gccgccgacg caccggcaga aatcaagatc
     1081 ggcacgctct atgcgtcatc cgggcgctac gcctcgattt cgatgccggt tcacagcgcg
     1141 ctgaagctct ggatcgacca gaaaaatgcc gatggcgggg tctatgtgaa ggcgttcgac
     1201 aagaaaattc ccgtcaagct tgtcgcctac gacgatcaga gcaacaccgc aaccgcgtcc
     1261 acgctctaca accagctgat cacgcaggac aaggtcgatc tgctggtcgc cgactccggc
     1321 tcggtcttga cggcgccggc ggtggcgatc gcgcgcgacc gcaagatgtt cctgttcgac
     1381 cagaccggaa ccggcgccag ctttttctcc aaggacaatc cctacatcgc gctgatggcc
     1441 gacccggtct cgaccatctg gccgaagccc gtcgcggact ttgtttcaca tgatggcccg
     1501 ggcctcggga tcaagaaaat cgccattctc tattcgacca acgaattcac gggaacgcag
     1561 gccaatgcct tccgcaaatt cgtcaaggat tccggtgcgc cgatcgagat cgtctacgat
     1621 cagggcattc ccaccgaaac caccaattac acggtcatca tcaacaacat caacaatacg
     1681 cagccggacg cggtgattca tttcggatac gcgccgaacg atattgcgtt cttgcgcaac
     1741 gtccaggacg tcggcgtcaa gttcaagatg ctattttcga tctatgctgg tctcgagacc
     1801 gagcttctcg aaaagaatgt cggcgagaag ggcctcgagc acgtcttcac ctatgtgccg
     1861 ccatcggaac tggaatatcc ggtcaatttc ggcatgacga tgaaggatta caaagccgcc
     1921 tgggacaaga aatatcccga tggcaagatc gagttcggct tcaatgcggt ggccggttac
     1981 accaccggtc tcgtcatcga gaagacactc tcggttgcga ccagcctcga ccaactcgaa
     2041 ctgcgccgcg cggtgttcag cctgtcggga gagctcaaga cgctcgatgg caccttcgcg
     2101 ctcgatgaaa ccggcggcca gatcggcgag ttgaccccgc tcgggcaact cgcgctcgac
     2161 gaacacgaac acatcaagtt catctcgatc tatccgcacg aaaccgccac cggcaagccg
     2221 gtctatccgc gtccatgatg cgattgcgga tgatgtaccg gatgcccgga gcggagtgtt
     2281 gatgctgact tacgcgctcg tcgccggcgt cctgttcggt ctttacttca gcctggtcgg
     2341 tatcggcctc aatctcgtgt tcggcgtcat gcgcatcgtc aatcttgcgc atggcgactt
     2401 tctgatgctt ggcgccttcg tagctttcgg cgtcgtgacg ttggccggca tcgatccctt
     2461 gttcgcggtg ccgctggcgt tcgtgatttt cgtcgtgatc ggcctgccgc tctattgggt
     2521 gctggtgccg cggctgcagg gttcggtgaa cccggaaatg ctatcgatca tcctgttctt
     2581 cgggttgtcg caggtgatcg aggcggtcac gacgatcttt ttcggcacca gcgaacgctc
     2641 gatccagagc cgtgcgctcg gcaccgtgtt ctcgaccatc aggataaaac tgttcggcgg
     2701 caggccggac gcggccggac cgatccagat ttttggacaa ggatttcctg cagcctgggt
     2761 gatcgccgcc attaccagcc tgatcgcgat ttcgctggtg tacgtctatc tgtaccgtac
     2821 ccgtctgggc accttgacgc gtgcggtgat gtcgcggcgc gacgaggcct tcgcgaccgg
     2881 catcgatgtc gatcgcgtct cggcggcggc attcggtatc ggattgggac ttgcggcggt
     2941 ggccggggta ttcgcgccgt tcatgttcgg atcggtgacg ccggcgctcg gcgccgacgc
     3001 gaccgtcacg tcattcgcca tcgtggtatt gggatcactc ggcaatcccc tggggactgc
     3061 gcttggcggc gtcgtctacg gcgtctgcta catgctggtg cagacctatc tgagttcatg
     3121 ggccgacctc ttgccctatg tgctcctgat tttcatcctg ctgctccgtc cgagcggcct
     3181 gctcggaagg caggtgcgcg ttgcctagcg ccttccgaca cgtgcttttc atcgtcgtgc
     3241 cgctgctcgc cgtctttgcg gtgctgccgg gcgtctacca aaatcatctg ctgctgttca
     3301 atttcgtgat cttcctgatc ctcgcccagg gcgtgaacat catctacggc tttacgggct
     3361 atctgccgtt cggctacgtc ggcttcttcg gcgccggcgc ctacggcttt gccatcatgg
     3421 tgatgcactt tcagacaccc gccatcgtcg cggtgctcgt cgccgggata gtcggcgtcg
     3481 cgctcgggct gttgctgacg ccattgctgc gtctctccgg cgcctatttc gcgatcgcca
     3541 atctcgcggc gtcgctggcg gtgcttcatt tcgtcgccaa tccggcgctg gagaatatca
     3601 ctcgcgggcc gtatggcgtt tcgctcaccg gcacgttcaa tccgacgctg gcctatgccg
     3661 ccgccctcgt ggtgctggcc ctgacgctct ccgccgtcgt attcctgaaa aactcccgct
     3721 ttggcctcgc attgcaggcg gtgcgggaag acgcggtctc tgcgtcgatg gcaggtgtgg
     3781 acgtcgtgcg gatgcgcgtg atcgcctggc tggcttcggc gctggtggcg ggtctcgccg
     3841 gcggggtcta tgcttggtat gtttcggtct tctatcccga caacgtcttt agcggcgaat
     3901 tcagcatctt tgccattgtg tttgcgctgt tcggtggcgt cgccacgatc acgggcccga
     3961 ttgtcggcgt catcatcctc tatggcatct acaatctgat cggtttcacc acgccgcaat
     4021 atttccagtt gatctacggg ctgttgatca tggggctggt gctgtttctc ccggccgggc
     4081 tggtttcgct cgcaacccga aggggctggc atgtcccttg atcgtgcgcc cattctcaac
     4141 gtgacaaagc tgacaaagcg cttcggcggg ttttacgcgc tcgacggctt gagcttccat
     4201 gtcgcgtccg gcgaaattct gggattggtg ggccccaacg gttccggcaa gaccaccgcg
     4261 atcaacgtga tttccggcct ttatgcgccc gacggcggcg aggtggtgct cgataatgcc
     4321 gatatcggcg gaatagcgtc gcacagactg gtccatcgcg gcatcaaccg caccttccag
     4381 atcccaaagc cgtttctgtc gctgacggtg cgcgagaata tctcggtcgc gctggcctat
     4441 ggccgcgccg cggtggcccc gccggcgatg gcggaattgc tcgacgaata tcggcttaac
     4501 gaggtcgccg accggccggc ggcggatctg aacaatgcgc agcagaagat gctcgatctg
     4561 gttcgcgcgc tcgccacccg tccgcggctg ctgctgctcg atgaactcgc cgctgggctc
     4621 aatccggcgg agctggactg gatcgccgaa cgcatcaagg cgctggcccg atccgggatc
     4681 gcgatcatcg tggtcgagca cctgatgggt ttcatcgaac acattaccga tcgcgtcatt
     4741 gtcatgaatg ccggcaagga gatcttcgaa ggcaagctcg cggtcgccgt gaaggtgccg
     4801 caagtcatcg aagtgttttt gggaggcgag catgccgcct gatgccgcac cgctgatgga
     4861 ggccaaaggc gtcgacgccg gctacggcac gatgcaggtg ctgtggggcg tcgatctcga
     4921 cgtccgcgcc ggcgaaaccg tgctgctgct cggcgccaat ggcgccggca aaactacttt
     4981 cctcaaatcg ctggtcggcc tgatcgaggc gcggcacggc agcatccagc ttgccggcga
     5041 ggatgtgacg cggatgcgtt ccagcgaccg gatgcggctc ggcatgacct acatgtcgga
     5101 actcgcggta tttcccgatt tgtcgatcga ggaaaacatc cgcgtcggcg cgcaggcgct
     5161 gggccatgcc aatccgggcg cccgtgccga agaactctac ggcgtatttc cggcgctgcg
     5221 cgaaaaacgc cgggcgccgg cgtccagtct ctccggcggg cagcgcaaga tgctcggcat
     5281 cgccaaggcg cttgccgccg agccgcggct gctggtgatg gatgaaccct cggccggact
     5341 gtcgccgctg tttgtcaagg aggtcatccg gattctcagc gatttgcgcg gccgcgggct
     5401 ggcgctcttg atcgccgagc agaatattgg tttcctcgaa gtggcgacgc gggtgtttgt
     5461 gctggaaggc ggccgcatcc ggttttccgg tacggttgcg gaaatgaccg ataatgaagc
     5521 gctgcggcgg gcttatttcg ggctgaaatg atgcgaacga tcagaatagg atccggggcc
     5581 ggctattcgg gcgatcgcat cgagcccgcg attgaactcg ccgagaaggg cgacatccag
     5641 tatctggtgt tcgagtgtct cggcgagcgc accgtcgcgc tggcgcagca ggcgcgcatg
     5701 aaaaaccctg atggcggata cgacccgctg ctcgaggaac ggatgcgcgc ggtgctgccg
     5761 atctgtgccg ccaaaggcat caagatcgtc accaatatgg gcgcggccca ttcggttgct
     5821 gcagcccgca agaccgccga gattgccaga tcgctcgggc ttgcgtcgct gaagatcgcc
     5881 gccgtcatcg gcgacgacgt gctggatgcc tgcaaagcgc gtgatctgcc gatcatggag
     5941 ttcgacggaa cgatcaaact gctcggcaac cggctattgt cggccaatgc ctatctcggc
     6001 gccgaaccga tcgccgatgc attgaccggc ggcgcggata tcgtgatcac cgggcgggcg
     6061 tcggatcccg cgctgtttct ggcgccgatg atccatgcct tcggctgggc gatggatgac
     6121 tggaatctct tggggcaggg cactgtcgcc gggcatttgc tggaatgcgc gggccagatc
     6181 accggcggct atttcgccga tcccggctac aaggatgttt tggatctggc gcggctcggc
     6241 tttccgatcg gcgaagtggg tgaagacggc tcgctcgtca tcaccaaggt cacgggctca
     6301 ggcggcgcgg tgacggcaca gacttgcaag gagcaactgc tctacgaggt gcacgatccc
     6361 acacgatatc tccagcccga cgtaacagcg gatttttcgc aagtgaaagt cgaggaaatc
     6421 gggc
//
LOCUS       DATNOI010000663         5391 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_3179,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000663 DATNOI010000000
VERSION     DATNOI010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3179"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..1418
                     /locus_tag="VN968_26645"
     CDS             81..1418
                     /locus_tag="VN968_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HXL32845.1"
                     /translation="MADEQLFEGLPEQERPQAEGRGALRLRVPERSQIGMQVAALDDL
                     ISEDHPVRSVWAFVEGLDLSALHDVIKAREGQPGHPPAAPELMLALWLWATVDGVGSA
                     RQLDRLCRDHLVYRWLCGGVSMNYHSLSNFRVAHMAVLERLLAQGVASMVQAGLVSVD
                     TLAQDGLRVRAGAGASSFRRRPRLGELHAAAQVRVERLRAEVETDPAAGDRRQRAAKE
                     RGARERAARIAAAQERMKELEAERERREKTNKKEVAKQKEPRASTTDADARVMKMADG
                     GWRPAYNMQIISAPEQQVIVAVDIDTSGSDRGLAQPGIETAHAEGYEPSNYLVDGGFT
                     KNDDIEWAHAKDIKMWCPAGQTKHGTDPYAPKKDDGVGVADWRQRMQGEDGKTFYRLR
                     AQHECINAHARRMGLRQLSLRGKIKARIHLLWFAVAHNMMRFFTLHAREQAAA"
     gene            complement(1642..2451)
                     /locus_tag="VN968_26650"
     CDS             complement(1642..2451)
                     /locus_tag="VN968_26650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXL32846.1"
                     /translation="MDDRVKVTMSDGIADVRLVRADKMNALDAAMFDALVATSGRLAH
                     EKGLRAVVLSGEGRAFCAGLDMDRFAAMKKGGNGVAGGESRDLTARTHGLTNFPQQAV
                     WGWRQLPVPVIAAIQGVAFGGGFQLALGADMRFLAPDARMSIMEIKWGLVPDMAGTPI
                     LASLVRDDILRELTYTGRIFSAQEALSYGLATRICDDPRAAAFEVAREIAGKSPEAIR
                     AAKRMLNNLAIDPGPALLAESVEQMKLMGGANQLEAVRANIDKRAPRFADV"
     gene            2560..3432
                     /locus_tag="VN968_26655"
     CDS             2560..3432
                     /locus_tag="VN968_26655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL32847.1"
                     /translation="MFNEQLLAGRRILVTGGGTGLGKSMAGRFLELGAEVHICGRRKG
                     VCDETATELMDLYGGRVTSHGVDIRNPLAVDEMIETIFTEGPLTDLINNAAGNFVSRT
                     QDLTPRGFDAIANIVMHGTFYVTHAVGRRWIAGKHPGNVVSITVTWVRNGSPYVVPSA
                     MSKSAIHAMTMSLAMEWGRYGIRLNTIAPGEIPTEGMSKRLNPGDEPGARTIARNPMG
                     RVGKMEELQNLAVFLISGGCDWINGETIAMDGGQALATGGNFYELRDWSDADWTQARE
                     SIKAQNEKDRTARG"
     gene            3571..5148
                     /locus_tag="VN968_26660"
     CDS             3571..5148
                     /locus_tag="VN968_26660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HXL32848.1"
                     /translation="MSDLQQPADLTDMLRARAKTRGDAIAFEFEGRQTSFAELDIQTN
                     RVANGLKALGVRPRERIGYLGKNSDIYFELLLGAMKANVVMAPVNWRLAGPEIAFIVE
                     DCKAPVLFVGPEFVEQIRNITSQLPSVRSFIATEGGAAGWQDYAAWRDAQSGDDPKLD
                     IDRQDIAIQLYTSGTTGKPKGAMLSHANFLNLVETGRDQKPDWNQWSTDDVSLVAMPI
                     FHIGGSGWGVMGLYHGAKGVIAREFDPTKVLDFFEQSAITKLFMVPAAMQFVVRQPRA
                     REVDFSRLKYMLYGASPIPAALLKECIEVFGCGFVQMYGMTETTGTIVALPPEDHVEG
                     LDRMRSAGKALPGVELAIVDAGGNRLPPGEVGEIATRSGSNMAGYWNLPEATARTLDS
                     EGWLRTGDAGYMDKDGYLYIHDRIKDMIISGGENIYPAEVESAICDHPDVAEVAVVGV
                     PDDKWGEAVKAIVVMKPGRKATSSDIIGFTRERIAGFKTPKSVDFIEALPRNASGKIL
                     RRHLRDPYWAGKDRQVN"
BASE COUNT         1064 a         1666 c         1713 g          948 t
ORIGIN      
        1 gctcctcaga gcgtgagaat gatttgccat agcggctatg ccgtgattct ctcgcattga
       61 gcgcgaagca ggggctggcg atggctgacg aacaattgtt cgagggttta cccgagcagg
      121 aaagaccaca ggccgaaggg cgcggcgcac tacggctgcg ggtgccggaa cgcagtcaaa
      181 tcggcatgca ggtggcggca ttggacgatc tgatttcgga ggatcatccg gtacgctcag
      241 tttgggcgtt cgtcgagggg ctcgatctgt cggcgttgca cgacgtcatc aaggcgcgcg
      301 agggccagcc cggacatccg ccagcagcgc cggaactgat gttggcgctt tggctatggg
      361 ccacggtcga tggggtcggc agcgcccgcc aactggaccg gctgtgtcgc gatcatctgg
      421 tctatcgctg gttatgcggc ggggtgtcga tgaactatca cagcctgtcg aacttccggg
      481 tcgcccatat ggcggttctg gaacggcttc tggcgcaggg cgtcgcgtcg atggttcagg
      541 ctggtttggt gtcggtcgac acgttggcgc aggatggctt gcgggtgcgc gcgggtgccg
      601 gggcgagttc gttccgccgg cggccgcggc ttggtgaact gcatgcagca gcgcaagtgc
      661 gggtggagcg cctgcgtgct gaagtcgaga cggatccggc ggcaggcgat cgtcgccagc
      721 gcgcggcaaa ggagcgtggc gcccgcgagc gcgcggcgcg gatcgcagcg gcgcaggagc
      781 gtatgaagga actggaggcc gagcgggaac ggcgagagaa gaccaacaag aaagaggtgg
      841 caaagcagaa ggagccgcgc gcttcgacca ccgatgccga cgcgcgggtg atgaagatgg
      901 ccgatggcgg atggcgcccc gcttacaaca tgcagatcat atcggctccg gagcaacagg
      961 tgatcgttgc cgtcgatatc gataccagcg gctccgatcg cggcctggca caacccggga
     1021 tcgagaccgc gcatgctgaa ggctacgaac cctccaacta tctggtcgat ggtggcttca
     1081 ccaaaaacga cgacatcgag tgggcccatg ccaaagatat aaagatgtgg tgcccggcgg
     1141 ggcagaccaa gcatggcacc gacccttatg cgccgaagaa agatgacggt gttggcgtcg
     1201 cggattggcg ccaacgcatg cagggcgagg acggcaagac attctaccga ctgcgcgccc
     1261 agcacgaatg catcaatgcc catgcccgcc gcatgggcct gcgccagcta tccctgcgcg
     1321 gcaagatcaa agcccgcatc catctgctgt ggttcgcggt cgctcacaac atgatgcgtt
     1381 tcttcacgtt gcacgccagg gagcaggcag cggcatagac aggggcgccc acgccagccg
     1441 gcgtccgcaa agcgctgtca gcatcggctg gcatcgggat cgcctggttt ccgcagtttg
     1501 cggaacttgg ccagatcgcc gtcattcggg cgccggctca cccgaaaaac ctgcggccgg
     1561 tgtcctccag cccatcaagg gcaaaattct tcgggcgaga gctaaacatt ctcatcctct
     1621 caggatgagg tctaatgtta gtcaaacatc ggcgaagcgc ggcgcgcgct tgtcgatgtt
     1681 ggcgcggacc gcctcgagct gattggcgcc acccatcagt ttcatctgct cgacagattc
     1741 agccaatagc gccgggccgg gatcgatggc cagattgttc agcatgcgct tggcggcgcg
     1801 gatcgcctcg ggactcttgc cggcgatttc gcgcgcgacc tcaaacgcag cggcgcgcgg
     1861 gtcgtcgcag atgcgtgtcg caaggccgta gctcagggct tcctgtgcgg aaaagatccg
     1921 ccccgtatag gtcagctcgc gcaggatatc gtcacgcacg agactggcca ggatcggcgt
     1981 ccccgccatg tccggcacca gaccccattt gatctccatg atcgacatcc gcgcgtcggg
     2041 cgcgagaaac cgcatgtcgg cgccgagcgc gagttgaaag ccgccgccga acgccacgcc
     2101 ctggatcgcg gcaatcaccg gcaccggcag ctgccgccat ccccataccg cctgctgcgg
     2161 gaaattggtc agtccatggg ttcgcgcggt cagatcgcgg ctttcgccac ccgctacccc
     2221 gttacccccc ttcttcatcg ccgcaaagcg atccatgtcg aggccggcgc aaaaagcccg
     2281 cccctccccc gataacacca ccgcccgcag tcctttttcg tgggcgagcc ggccgctggt
     2341 tgcgaccagt gcgtcgaaca tcgcggcatc gagcgcgttc atcttgtccg cccgcaccag
     2401 ccggacgtcg gcgataccat ccgacatcgt gaccttgacg cgatcatcca tgatgagtct
     2461 cccctgctct tcttcgttgt tgccgcttta cagaaaggcg ttgtgcggat ttagtcaatc
     2521 aaccaattaa cagacaattt tccagggtgg aaacaacaga tgttcaacga acagcttctt
     2581 gccgggcggc gcattctggt caccggcggc ggaaccgggc tcggcaaatc gatggccggg
     2641 cgcttcctcg agttaggcgc cgaggttcat atctgcggcc gccgaaaggg cgtctgcgac
     2701 gaaaccgcga ccgaattgat ggacctctat ggcggcaggg tgacgagcca cggcgtcgac
     2761 atcagaaacc cgctcgcggt cgacgagatg atcgagacca tcttcaccga aggcccgctc
     2821 accgatctca tcaacaacgc ggccggaaat ttcgtctcgc gcacccagga cctcacccct
     2881 cgcggcttcg atgcgatcgc caacatcgtc atgcatggca cattctatgt gacgcacgcg
     2941 gtgggccggc gctggatcgc cggcaagcat cccggcaatg tggtgtcgat taccgtgacg
     3001 tgggtgcgga acggcagccc ctatgtggtg ccatcggcga tgagcaaatc cgcgatccac
     3061 gccatgacca tgtcgctggc gatggaatgg ggacgctatg gcattcggct caacaccatt
     3121 gcgcccggcg aaatcccgac cgagggcatg agcaagcgcc tcaatcccgg cgatgaaccc
     3181 ggcgcgcgca ccattgccag aaacccgatg ggtcgcgtcg gcaaaatgga ggaactgcag
     3241 aacctcgcgg tgttcctgat ttcgggcggc tgcgactgga tcaacggcga gaccatcgcg
     3301 atggatggcg gccaggcact ggccacgggc ggaaactttt atgagttgcg cgactggtcc
     3361 gacgccgact ggacccaggc ccgcgagtcg atcaaggcgc agaacgaaaa ggaccgcacc
     3421 gcgcgggggt gattccaagc ctcatgcggg aacctcatcc tgaggagcgc gctcttgcgc
     3481 gcgtctcgaa ggatgaagtt gtacatcctg atggttcgag acggcgcaag tgcgcctcct
     3541 caccatgagg gccaaactta cgggagaaac atgtccgacc tccagcaacc ggcagacctc
     3601 actgacatgc tgcgcgcgcg ggcgaagacc cgcggcgatg ccatcgcctt tgaattcgaa
     3661 ggccgccaga ccagctttgc cgagcttgat atccagacca atcgcgtcgc caacggtctg
     3721 aaggcgctcg gcgtccgccc tcgcgagcgc atcggctatc tcggcaagaa cagcgacatc
     3781 tattttgagc tgctgctggg cgcgatgaag gccaatgtgg tgatggcgcc ggtgaactgg
     3841 cggctggccg gacctgaaat cgccttcatc gtcgaagact gcaaggcgcc ggtgctgttc
     3901 gtcgggccgg agttcgtcga acagatccgc aacatcacat cgcagcttcc aagtgtccgc
     3961 agcttcatcg ccaccgaagg cggcgcggcg ggctggcagg actatgcggc ctggcgcgac
     4021 gcgcagagcg gcgacgatcc taagctcgac atcgacaggc aggacatcgc gatccagctc
     4081 tacacctccg gcacgacagg aaagccgaaa ggcgcgatgc tgtcgcacgc caattttctc
     4141 aatctggtgg aaaccggcag ggaccaaaaa cccgactgga accaatggtc gaccgacgac
     4201 gtctcgctgg tggcgatgcc gatctttcac atcggcggct ccggctgggg cgtgatgggt
     4261 ctttatcacg gcgccaaggg cgtgatcgcg cgcgaattcg atcccaccaa ggtgctcgat
     4321 ttcttcgagc aatctgcaat cacaaaattg ttcatggtgc ctgccgcaat gcaattcgtg
     4381 gtgcggcagc cgcgcgcgcg cgaggtcgat ttttcacgcc tgaagtacat gctctatggc
     4441 gcctcgccga ttcccgcagc cctgctgaag gaatgcatcg aggtgttcgg ctgcggcttc
     4501 gtgcagatgt acggcatgac cgaaaccacc ggcaccatcg tggcacttcc gccggaagat
     4561 catgtcgagg ggctggaccg gatgcgctcg gccggcaagg ccttgccggg cgttgagctt
     4621 gcaatcgtcg atgccggcgg caaccgattg ccgccgggcg aagtcggcga gatcgcgacg
     4681 cgttccggct cgaacatggc cggctactgg aatctgccgg aggcgaccgc caggaccctc
     4741 gacagcgagg gctggctgcg taccggcgac gccggctaca tggacaagga cggctatctc
     4801 tatatccacg accgcatcaa ggacatgatc atctccggcg gcgagaatat ctatcccgcc
     4861 gaagtcgaaa gcgcgatctg cgaccatccc gatgtcgccg aagtcgccgt ggtcggcgtg
     4921 cccgacgaca aatggggcga agccgtcaag gcgatcgtgg tgatgaagcc cggcaggaag
     4981 gcgacctcgt ccgatatcat cggcttcacc cgcgaacgca tcgcgggctt caagacaccg
     5041 aagtcggtcg acttcatcga agccctgccg cgcaatgcct ccggcaagat cctgcgccgg
     5101 catctgcgcg atccctactg ggcgggcaag gaccggcagg tgaattgaga tgttgaaatt
     5161 gtcctacaga cgtcatcccc cgcgaaagcg ggggatccag tattccagag cgttgagatt
     5221 tcatgaatcg cggtgattac tggatacccg catgcgcggg tatgacggtc aacagcggtg
     5281 gagcgcagtg ccccctcaat gcttcccttc ccccatatag ccgaacagga atcccgcgac
     5341 cttgcgcttc tggatttcct cgctgccttc ggtgatgcga tagcggcggt g
//
LOCUS       DATNOI010000664        17384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_771545,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000664 DATNOI010000000
VERSION     DATNOI010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17384
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771545"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..3637)
                     /locus_tag="VN968_26665"
     CDS             complement(74..3637)
                     /locus_tag="VN968_26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL32849.1"
                     /translation="MAVPAPPINPELRPGSLSIVRRVGSGAEAGFETFIKITADGSVT
                     AFNGHVDLGTGIRTALGQIVAEELDVSFARVVVVLGDTSLVPNQGATIASETIQITAV
                     PLRKAAAQARQFLIVRAAERLELSVDDLAIEDGLIRGRDNRSVSYGELIAGEDVRLEL
                     AENVPVKAVESYTIVGQSVPRTDLLAKATGELVYVHDVRVPGMLHGRVVRPPYAGVDA
                     GAFVGTSLIAVDEASVADIPGLVAVVTIGDFVGVIAEREENAVKAAAQLKVIWKPTPT
                     LPDLNDIETALRTHPSAPRTLIDKGDVDAAIAAAAKPMPRTYIWPYQMHGSIGPSCAV
                     ADYREGHVRVWSGTQNPHILRTDLALLLRRPEPEIEVTRLEAAGCYGRNCADDVSADA
                     VLLSRAAGRPVRVQLTREQEHAWEPKGTAQLMDVKGGLNADGSVAGYDFATRYPSNGA
                     PTLALLLTGAITPVAEVSEMGDRTAIPPYDYDAMRVVAHDMPPIVRASWFRGVSALPN
                     TFAHESYIDELATEAGVDPIEYRQRYLRDSRAIDLVNAVAERAGWTPRPVWNEPIAEG
                     DIVRGRGFAYAQYVHSKFPGFGAAWSAWIADVAVNKATGDVSVTRVVAGQDSGLMINP
                     DGVRHQIHGNVIQSTSRALMEEVSFDHNAVTSREWGAYPIITFPDIPKIDVLMLPRQH
                     QPPLGVGESASVPSAAAIANAIFDATGVRFRELPFTPERILAGLRGEQPVAASALPRT
                     PPKPAASSDKWQNPFVRRRGALASAAALVAAMVGVAAAVLPWRSIAPIARPDASVFSA
                     ATIARGRQLAALGDCAVCHTKANGVINAGGRAIETPFGIIYSTNITPDVETGLGAWSY
                     PAFERAMREGIHRDGRHLYPAFPYPHFAKATDADLQALYAYLMAQAPVRADNPKTTLA
                     LPFNWRPLMAGWNALFHRTGVFEAYSTKSEIWNRGAYLVEGLGHCGACHSPRNALGAE
                     KTSAYLAGGFAEGWEAPPLTSLSQAPIPWSEDELFAYLRTGESHFHGVAAGPMAPVVK
                     ELARLPDHDIRAMAVYLSAFNTKAISKPGQDQLATRLETLTRTKVVSASALGARLYQG
                     ACAVCHQVGGAPLFGSRPSLALNSNLHSAVPDNLIQVILHGIASPASSDLGYMPAFKG
                     SMTDDQLAELVSYLRQQFAPDKPPWTEVHAAVGRARQRISH"
     gene            complement(3637..4119)
                     /locus_tag="VN968_26670"
     CDS             complement(3637..4119)
                     /locus_tag="VN968_26670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL32850.1"
                     /translation="MTQTAIRLTVNGRIHDVSAAPDTALLYVLRNDLELNGPKYGCGL
                     GECGACAVLIDGLTARSCVIPISGCVGRDIVTLEGLGSRDHPDPVQDAFIREQAAQCG
                     YCLNGMIMTTKALLMRTPHPSEADVLEALRYNLCRCGAHIEIVRAAMRAAGLVAEAQG
                     "
     gene            4466..5917
                     /locus_tag="VN968_26675"
     CDS             4466..5917
                     /locus_tag="VN968_26675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxynicotinate reductase"
                     /protein_id="HXL32851.1"
                     /translation="MPTDATAAVDDKIRCDACPVMCYIKPGAAGACDRYANHNGELVR
                     VDPHIVLERTVSHGGRLVPFQASGDWDGKIVHEPSMFVTAIGAGTTYPDYKPAPFIVS
                     SEIDGVDMVTVVTEGIFSYCGVKVKIDTDRYLGPETATVRAQGEAIGHVTTGEYGSQM
                     LSLGGVHHLTGGSKKEGRVTCDTLMDLSNCKPVELAIDGGATVVVQAGYPPIVNGVAE
                     ERMRVGCGSATIGMFAKQWHGKVDEVVVVDDHITGVLSEHQAGKLLGIPDTGIKMKGR
                     RSTPGRYFQVAEPGTGWGGTMISDPLSVLGPFDPRKAHPGTTMLMVSTTGEHAAYYEL
                     DEALKPIEKPMPVDLRLSVERIQENCEPALCTVLFMGGAGGSLRAGVTDNPVRLTRSV
                     KDALTRVTSGGAPVYVWPGGGITFMVDVTRLPAGAFGYVPTPALVAPIEFTLKLSDYA
                     ALGGHMDYVRPLSSLKDNTEIRQMPQSPSGQLT"
     gene            5914..6825
                     /locus_tag="VN968_26680"
     CDS             5914..6825
                     /locus_tag="VN968_26680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HXL32852.1"
                     /translation="MTRVPQIGWLADGRRLHLQDGPIDLIVAVHGSETNRRAAYDAAA
                     RRFTGLLEELCDELPLLRRAADPARCELQGAVARRMDAAVAPFALDHFITPMAAVAGA
                     VAEEILGAMLDAARLERSYVNNGGDIALHLGHGEHFTVGLMDRPDRHGLMRTIIVDAV
                     DPTRGVATSGRHGRSFSLGIADAVTVLARTASQADAAATIIANAVDLPGHPAILRCPA
                     HDLQPDSDLGARLVTRDVGELTGHEIGRALESGAACARKLLAAALIDGAALRLQGETV
                     LVSARAIGTLRSGTPVHRGAMESAMHV"
     gene            6844..7428
                     /locus_tag="VN968_26685"
     CDS             6844..7428
                     /locus_tag="VN968_26685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HXL32853.1"
                     /translation="MSAIIRKIVTVVEETRLEIGQVVTPPTRRAAAIAVIENPFAGRY
                     VEDLSPLIVIGEELGELLSKKAVAALGIDGTKVQSYGKAVAVGENGELEHAAAILHPK
                     MGAPVRKVLGKGAALIPSSKKRGGPGTTLDIPLGHKDAAFVRSHFDGMEVQINDAPRA
                     NEIMVAVAVTDSGRPLPRVGGLTIAEIKGEDGLR"
     gene            7457..8662
                     /locus_tag="VN968_26690"
     CDS             7457..8662
                     /locus_tag="VN968_26690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32854.1"
                     /translation="MQRSRIFLGAGLAIMFSGMAHSAMAQSEIKIGEINSYSLLPAFT
                     EPYRKGWQLAVEEINAAGGINGKKLVVVSKDDGGKPADAQTAANELVSSENVAMLAGA
                     FLSNIGLAVSDFANQKKVFFLAAEPLTDAITWAKGNRYTFRLRPSNYMQAAMLVEEAA
                     KLPAKRWATIAPNYEYGQSAVAVFKKLMSEKRPDIQWVDEQWPPQGKIDAGPVVQAVA
                     AANPEAILNVTFGADLIKLVREGNTRGLFKDRAVVSFLTGEPEYLDPLKEETPEGWIV
                     TGYPWYSIKTPEHDAFIKAYQAKYNDYPRLGSIVGYQTIKSAAAILAKANSTDPEKLI
                     AAAEGLSVPSPFGEITFRKIDHQSTLGAFVGKTALRGGKGIMVDSVYRKGSDYLPSDA
                     EVAKLRPKE"
     gene            8718..10616
                     /locus_tag="VN968_26695"
     CDS             8718..10616
                     /locus_tag="VN968_26695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL32855.1"
                     /translation="MAFYFVQFLTGLASAASLFLVASGLSIIFGVTRIVNFAHGAFYM
                     LGAYVAFTLTERFSGAIGFWGGIVVAALIVAAVGVLIEMVLLRRIYHAPELFQLLATF
                     GLTLMVEDLVVLIWGPSDLLGRRAPGFKGAVDFFGQNVPTYDLFLIGLSPVVLGILWL
                     LFQRTRWGVLVRAATQDRDMVAALGVNQKWLFTSVFAVGVFLAAFGGALQIPRDAVHH
                     ALDLRIIVDVFVVVVIGGLGSIIGAFVAAVLVSELNAFGILIFPKISIILVFLVMAAV
                     LIVRPWGLFGKPEARARRTPGLTVNPWRPLTSNERLAAMAALVLAAALPLIGGNYALT
                     VGSEIAIFVIFATSLHFLMSVGGLASFGHAAYFGLGAYGVALLAKLAGLPMILSLLLG
                     PLLGCLGAAVFGFFAVQLSGVYFAMLTLAFAQIVWSIAFQWVEVTGGDNGILGVWPET
                     WAAAPAHFYWLSIGVAALAVAALRIIVFSPFGYALRATRDSPLRSEAIGIDGKRIQWT
                     AFVIAGTVAGIAGALFAYLKGSVFPDSLGISLSVDALVMVLLGGVETVSGAVVGAIVY
                     KALSIWLVSQTDLSKLVLGGVIVLIVVAFPKGIVGMLETIRNRRRGEAAKPESSKPAL
                     APGIETAE"
     gene            10613..11404
                     /locus_tag="VN968_26700"
     CDS             10613..11404
                     /locus_tag="VN968_26700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32856.1"
                     /translation="MSIAPTLLSVEGLSKSYGGVHAVRNVSFALKAGEILALIGPNGA
                     GKSTCFDMLNGQNIPDSGRISLLGQDTTGKKPRAIWRLGVGRTFQITATFPTMTVREN
                     VQVALVSYRRQLFNLRASTQWFAKQEAGRLLELVGMSGYAERPCGELAYGDLKRLELA
                     IALANQPKLLLMDEPTAGMAPRERIELMRLTAQIARQQSIGVLFTEHDMDVVFEHADR
                     ILVLNRGTLIAEGTPEEVRRNPQVRAIYLGEGLVYDISHREGAPS"
     gene            11401..12105
                     /locus_tag="VN968_26705"
     CDS             11401..12105
                     /locus_tag="VN968_26705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32857.1"
                     /translation="MKLSVQDLNSHYGPAHILFDIALEVDDGEVVALLGRNGAGKSTT
                     FRSIVGLVEQRTGRIIFEGKDVSDQPTHAIVRGGLGYVPEERRIFTDLTVDENLEVGR
                     QPIRPNAPHWTREKLFALFPNLGEMRGRPGGRMSGGEQQMLTIARTLMGNPSLVLLDE
                     PSEGLSPKIVEQMVDAILTMKKEGVSIVVSEQNLHFARLISDRAYIIERGRMCFSGTM
                     SELDARPDVRDAHLAL"
     gene            12138..12593
                     /locus_tag="VN968_26710"
     CDS             12138..12593
                     /locus_tag="VN968_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HXL32858.1"
                     /translation="MTRSVSPKRSMKPSRPNYVLEEQVGFILRQVWQRHAAIFAREIG
                     INLTPTQWAALAKLTETGPCSQNQLGRLTAMDVATIKGVIDRLSARGLTETSPDPEDG
                     RRLLVSLTRAGQQMAEKAAPSALAITRETLAPLDPKERETLIALLSKLR"
     gene            complement(12710..13486)
                     /locus_tag="VN968_26715"
     CDS             complement(12710..13486)
                     /locus_tag="VN968_26715"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32859.1"
                     /translation="MIKPSEDEGAQRGHVKRRKFIAALGVAAFWPLAACAQQPKTVTI
                     GILVPGNLDPAPFLSVFKEELRRLGYLEGQNLCFEFRTAKGKMDALPGLAAELVGAKA
                     DVIVTWLTPAVRAAKQATSQIPIVMAGAGDPVATGVVASLVRPGGNITGMAGVTAELA
                     GKNVELIREMLPSAKRLAVLCNTADLFTKPFLAQIEGAASGTGFELDRIMVNGNDELE
                     TSFPRMNKDGVDAVIVQPSLPTTRAAELALGPHVQSPHRW"
     gene            13600..15221
                     /locus_tag="VN968_26720"
                     /pseudo
     CDS             13600..15221
                     /locus_tag="VN968_26720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711250.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
     gene            complement(15359..16327)
                     /locus_tag="VN968_26725"
     CDS             complement(15359..16327)
                     /locus_tag="VN968_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL32860.1"
                     /translation="MSHTVRSLSTSGLSPKKQIQCWTDALTELCGQFDVDPLEASSLE
                     GQINYTTVSRLKLCQIEASQHRLAHPVSRAKSGEHPYVKILFQTYGISHFEQSGRRIE
                     LVPGDCLAYDVSCPHTIISPSLTRHEVVIVPKELLRERGFHSAKMSPCKLSARTGTGR
                     IAYDFVHAAFGEAEKLSPYNAIGVADSLIDLLLLPLRQAGTPLDCSGPVAMYVRAQAF
                     IREHLRDPDLSIDQISAALGCTKRYLHMLFSDRGMTVSDYIWQARLQNCRQELEAQGG
                     KTITDVAFSWGFSSSSHFSRVFRKYFGIVPSSIHKAQHGSLSPDLP"
     gene            16887..>17384
                     /locus_tag="VN968_26730"
     CDS             16887..>17384
                     /locus_tag="VN968_26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXL32861.1"
                     /translation="MRKVLFATCFGAVATFAAGAATANDELGKMSQNPKDWVMPAGSY
                     DNQRYSKLNQINAANVGKLQVAWTFSTGVLRGHEGGPLIVGNMMYVHTPFPNKVYALD
                     LSDENKIVWKYEPKQDPNVIPVMCCDTVNRGVAYGDGKIFLHQADTTLVALDAKTGKV
                     EWTAKN"
BASE COUNT         3263 a         5202 c         5647 g         3272 t
ORIGIN      
        1 atgtctgtga ctgccataag cgaactctat cttgccggaa gccttgcaac cagtggcttt
       61 taggccgggc aggtcagtgc gatatccgtt gtctggcccg gccgacggcc gcgtgcactt
      121 cggtccaggg tggcttatcg ggagcgaatt gctggcgcaa ataggacaca agctcggcga
      181 gctgatcgtc ggtcatgctg cccttgaagg cgggcatgta gccgaggtcg cttgacgccg
      241 gcgatgcgat gccgtgcaaa atcacctgga tcagattgtc cggcacagcg ctatgcaagt
      301 tgctgttcag cgccaacgac ggccggctgc cgaacagcgg cgcgcctccg acctgatggc
      361 aaaccgcgca ggcgccttga tagagccgcg cgccaagtgc cgatgccgaa acgacttttg
      421 tgcgggtcaa ggtttcgagt ctggtggcca gttggtcctg gcctggtttg ctgattgcct
      481 tggtgttgaa tgcgctgagg tagaccgcca tggcgcgaat gtcgtggtcg ggcagacgag
      541 cgagttcctt caccaccggc gccatcgggc ccgcggcgac accgtgaaag tgggattcac
      601 cggttcgcaa ataggcgaac agttcgtcct cactccaggg gatcggcgcc tgcgaaagcg
      661 aagtcagcgg cggcgcttcc cagccttcgg cgaagccgcc ggccagatac gcgctggtct
      721 tttccgcgcc gagcgcattg cgcggcgaat gacaggcacc gcaatggccg aggccttcga
      781 ccagataggc gccgcgattc caaatttctg atttcgtcga ataggcttcg aacacgccgg
      841 tacgatgaaa cagcgcgttc catccggcca tgagcggacg ccaattgaac ggcaaggcaa
      901 gcgtggtttt ggggttgtcg gcacgcaccg gcgcctgtgc catcagatag gcgtagagcg
      961 cctgcagatc ggcatccgtg gccttggcga aatgcggata gggaaacgcc ggatagagat
     1021 gccggccgtc gcgatggatg ccctcgcgca tggcgcgctc gaaggcggga taggaccatg
     1081 cgcccagccc ggtttcgaca tcgggcgtga tgttggtgct gtagatgatg ccgaatggcg
     1141 tctcgatggc gcggccgccg gcattgatga cgccgttcgc cttggtgtgg cagaccgcgc
     1201 agtcgccaag ggcggccagt tgccggccgc gggcaatggt tgccgccgaa aacaccgatg
     1261 cgtcgggccg cgcgatcggc gcaatcgacc gccatggcag cacggctgcg gcgacgccga
     1321 ccatcgcggc aaccagcgca gccgcacttg cgagcgcgcc gcgccgcctg acaaacggat
     1381 tctgccattt gtcgctcgaa gcagcgggct tgggcggcgt tcgcggcaat gcgcttgctg
     1441 ccacgggttg ttcgccccgc aatcccgcaa gaatgcgttc cggcgtgaac ggcaattcgc
     1501 gaaagcgcac cccggtcgca tcaaagatcg cattggcgat cgccgcggcg ctcgggaccg
     1561 acgcggattc gccgacgccg agcggcggct gatgctgtcg cggcagcatc aagacgtcga
     1621 tcttcgggat gtccgggaag gtgatgatgg ggtaggcgcc ccattcgcgg ctcgtcaccg
     1681 cgttgtggtc gaacgagacc tcctccatca gcgcgcggct ggtcgactgg atgacgttgc
     1741 catggatctg gtggcggacg ccgtccgggt tgatcatcag cccggaatcc tggccggcga
     1801 ccacgcgggt cacactgaca tcgccggttg ccttgttgac ggcgacgtcg gcaatccagg
     1861 ccgaccacgc cgcgccaaaa cctgggaact tgctgtgaac atactgcgca taggcaaaac
     1921 cccgtccgcg cacgatgtca ccctcggcga tcggctcgtt ccaaaccggg cgaggggtcc
     1981 agccggcccg ctcggcaacc gcgttgacga gatcgatcgc cctggaatcc ctgagataac
     2041 gctggcggta ttcgatcgga tcgacgccgg cttcggtggc gagttcgtcg atataggatt
     2101 catgcgcaaa ggtatttggc aacgccgaga cgccacggaa ccacgatgcc cgcacgatcg
     2161 gcggcatgtc gtgtgcgacc acccgcatgg catcgtaatc gtaaggcggg atcgcggtgc
     2221 ggtcgcccat ctcggacact tccgcgaccg gcgtgatcgc accggtcaac agcaacgcca
     2281 gggtcggcgc gccgttggac ggatagcggg tggcaaaatc gtagccggcg acactgccgt
     2341 cggcgttcag tccacccttg acgtccatca gttgcgcggt gcccttcggc tcccaggcgt
     2401 gttcctgctc gcgcgtcagc tgcactcgga ccggacggcc ggccgcgcgc gacagcagca
     2461 ccgcgtcggc ggaaacgtcg tcggcgcaat tgcggccata gcacccggct gcctccagcc
     2521 gagtcacctc gatttcgggt tccggccttc gcagcagcag cgcgaggtcg gtgcgcagga
     2581 tgtggggatt ttgcgtgccg gaccagaccc gtacgtgacc ttcccgataa tccgcgacag
     2641 cgcaggacgg gccgatcgaa ccgtgcatct ggtacggcca gatatacgtc cgcggcatcg
     2701 gcttggcggc ggcggcgatc gcggcatcga cgtcgccctt gtcgatcagg gtccgcggtg
     2761 ccgagggatg ggtgcgcagc gcggtctcga tgtcgttcag atcgggcagt gtcggcgtcg
     2821 gcttccaaat caccttaagc tgcgctgcgg ccttgactgc gttctcctcg cgctcggcga
     2881 tgacgccaac gaaatcgccg atcgtgacga cggcgaccag ccccggaata tccgccaccg
     2941 aggcttcatc gaccgcgatc aggctggtgc cgacaaaagc accggcatcg acgccggcat
     3001 agggcggacg aaccacgcgg ccgtggagca tgccgggcac gcgcacgtcg tgaacgtaga
     3061 ccagttcgcc ggtggccttc gccaaaaggt cggtacgcgg caccgactgg ccgacgatgg
     3121 tgtaagactc gaccgctttt accggaacat tttcagccag ttccaggcga acgtcctcgc
     3181 cggcaatcaa ttcaccgtag ctgacgctgc gattatcccg gccgcggatc aggccgtcct
     3241 ctatcgcaag gtcgtccacg gaaagttcca gtcgttcggc ggcgcgcacg atcaggaatt
     3301 gccgcgcctg cgctgccgcc ttgcgcaacg gtaccgccgt tatctggatg gtttcactgg
     3361 cgatcgtcgc accctggttg gggaccagtg aggtgtcgcc gagcacgacg acgacgcgcg
     3421 caaaggacac gtcgagttcc tcggcgacga tttgtccgag cgccgtccga atgccggttc
     3481 cgagatcgac atgcccgttg aaggccgtga ccgaaccgtc ggccgtgatc ttgataaagg
     3541 tttcgaatcc ggcttccgcg cctgacccga cccggcgaac gatagacaag gacccgggcc
     3601 ggagttcggg attgatcggg ggggctggca ccgccatcaa ccctgtgcct ccgcgacaag
     3661 tcccgccgcc cgcatcgccg cgcgcacgat ttcgatatga gcgccgcacc ggcataaatt
     3721 atatcgaagc gcttcgagca cgtcggcctc ggaagggtga ggggtgcgca tcaatagcgc
     3781 cttggtggtc atgatcatgc cgttgaggca gtagccgcat tgcgcggcct gttccctgat
     3841 aaaggcgtcc tgcaccggat cgggatggtc gcgcgagcca aggccttcga gcgtcacaat
     3901 gtcgcgcccg acgcagccgc tgatcgggat cacgcaggaa cgcgccgtca ggccgtcgat
     3961 cagtaccgcg caggcgccgc attcgccgag cccgcagcca tatttcggtc cgttcaattc
     4021 aagatcgttg cgcagcacgt aaagcagcgc ggtgtcgggg gcggcgctga cgtcatgaat
     4081 cctgccgttg acggtcaggc ggatcgcggt ctgcgtcatg ctggtggtct cggggccaaa
     4141 tggtcgtgct gcaaacgtcg cgttaagccg gtgggccaag gataccgttt gtatacaaac
     4201 gtgcaagcgc actgccccgc agcgaacctt tgcccggttt atgaacagcg atgccgggca
     4261 acctgcgggt ttattcggca gcggcgtttt cggggtccgt cggcgcttga caggcttcct
     4321 cggcgcgagc aacatcgttc gtatacgaac aaaatgaccg gtggcgagag acacattatc
     4381 atccaaggcg ccgcccgaac tttgatacgg ggcttcgtta tcgatgtggc ttatgaccgt
     4441 ttgacgctgg cttgtgagct tgttcatgcc gactgatgcg acggctgccg ttgacgacaa
     4501 gatccgctgc gatgcctgtc cggtgatgtg ctacatcaag ccgggggcgg caggtgcctg
     4561 tgatcgctat gccaatcaca atggcgagtt ggtgcgcgtc gatcctcata tcgtgctgga
     4621 gcgaacggtg tcccacggtg gacgcctggt gccgttccag gccagtggcg actgggacgg
     4681 caagatcgtt catgagccga gcatgttcgt gaccgctatt ggcgccggca ccacctatcc
     4741 cgactacaag ccggcgccgt tcatcgtgtc ttcagagatc gatggcgtcg acatggtcac
     4801 cgtggtgacc gagggcatct tcagttattg cggcgtcaag gtgaagatcg acaccgaccg
     4861 ttatctcggt cctgagacgg cgacggttcg cgcgcagggc gaggcgatcg gccacgtcac
     4921 cacaggcgaa tacggctcgc agatgctgtc gctgggcggc gttcatcatc tgaccggcgg
     4981 ctccaagaaa gaaggccgcg tcacctgcga cacgctgatg gatctgagca actgcaagcc
     5041 ggtcgaattg gcgatcgacg gcggcgccac cgtggtggtg caggccggct atccacccat
     5101 cgtcaatggc gtggccgagg agcgcatgcg ggtcggttgc ggctcggcca cgattggcat
     5161 gtttgccaag cagtggcacg gcaaggtcga tgaagtcgtt gtcgtggatg accacatcac
     5221 cggcgttctc tcggagcatc aggccggcaa actgctcggt attcccgata ccgggatcaa
     5281 gatgaaggga cggcgctcga caccgggccg ctatttccag gtggccgagc ccggcaccgg
     5341 ctggggcggt acaatgatct ccgatccgct ttcggtgctg ggtccgttcg accccaggaa
     5401 agcgcatccc ggcacaacca tgctgatggt ctctacgacc ggcgagcatg cggcttacta
     5461 tgaactcgac gaggccctga agccgatcga aaagccgatg ccggtcgatc tcaggctgtc
     5521 ggtcgaacgg atccaggaga attgcgaacc ggcgctgtgc acggtgttgt tcatgggcgg
     5581 ggcgggcgga tcgttgcggg cgggtgtcac cgacaatcca gtgcggctga cgcgatcggt
     5641 gaaggatgcg ctgacgcggg tcacgagcgg cggcgcgccg gtatatgtct ggccgggtgg
     5701 cggcatcacc ttcatggtgg atgtcacgcg cttgccggcc ggcgcctttg gctatgttcc
     5761 gacgccggcg ctggtcgcgc cgatcgagtt tacgctaaaa ctctctgact atgcggcgct
     5821 gggcggccat atggattatg tgcgtccgct ttcgtcactg aaggacaaca ccgaaatccg
     5881 tcaaatgcct cagtctcctt cgggacagct cacatgacga gagttccgca gatcggatgg
     5941 ctcgcggacg gcagacggct tcatctgcag gacggtccga tcgacctgat cgtggcggtt
     6001 cacggcagcg agacaaatcg gcgtgccgcc tatgacgccg cggcgcggcg ttttaccggt
     6061 ctgctcgaag agctctgcga cgagttgccg ctgttgcgcc gggcggccga cccggcgcgt
     6121 tgcgaactgc aaggtgcggt cgcacgccgc atggatgccg ccgtggcgcc gtttgctctt
     6181 gaccatttca ttacgccgat ggcggcggtg gcgggcgccg tcgcggaaga aattcttggc
     6241 gcgatgctgg atgcggcgcg gcttgaacgc agctatgtca acaatggcgg cgacatcgcg
     6301 ctgcatcttg gccacggaga gcatttcacc gtgggcctga tggaccggcc tgaccggcat
     6361 ggcctgatgc gaaccatcat cgtcgatgcc gtcgatccca cccgaggggt tgccaccagc
     6421 ggacgccacg gacgcagttt ctcgctcggc atcgccgacg ccgtcaccgt gctagcgcgg
     6481 acggcatcgc aggctgacgc cgccgccacc atcattgcca acgcggtcga tttgcccgga
     6541 catcccgcca tcctgcgctg tccggcgcat gatctgcagc ccgacagcga tctcggcgca
     6601 cgcctggtca cccgcgacgt cggcgaattg acaggacatg aaatcgggcg cgcgctcgaa
     6661 tccggcgcgg cctgcgcgcg caagctgctc gcggcggcat tgatcgacgg cgccgcattg
     6721 cgcttgcagg gcgagaccgt cctggtatcg gcgagagcga tcggcacgct tcgatcggga
     6781 acgccggtcc accgaggcgc gatggaaagt gcgatgcatg tctagagaac gaggttgaca
     6841 acgatgagcg cgatcattcg caaaatcgtc acggtcgtcg aagagacgcg gctggagata
     6901 gggcaggtgg tcacgccgcc gacccggcgc gctgcggcga tcgcggtgat cgaaaatcca
     6961 tttgccggca gatatgtcga agacctttcg ccattgatcg tgataggcga ggagctcggc
     7021 gagctgcttt cgaaaaaggc cgtcgccgcg ctcgggatcg acggcacgaa ggtgcaaagt
     7081 tacggcaagg cggtggcggt cggcgaaaac ggtgaactcg agcacgcggc ggcaattctg
     7141 catccgaaga tgggtgcgcc ggtgcgcaag gtgctgggca aaggcgccgc gttaattcca
     7201 tcgtcgaaaa agcgcggtgg accggggacg acgctggata ttccgctcgg ccacaaggat
     7261 gcggcgttcg tgcgcagcca cttcgacggc atggaagtgc agatcaacga tgcgccccgc
     7321 gccaatgaga tcatggtggc ggtggcggtc accgacagcg gcaggccgct gccgcgcgtc
     7381 ggcgggctga cgattgccga gataaagggt gaagacggtc tgcgataact ggtttcttca
     7441 acattggaga tttggaatgc aacgaagcag aattttctta ggagccgggc tcgcgatcat
     7501 gttcagcggc atggcgcatt ccgccatggc gcagagcgag atcaagattg gcgaaatcaa
     7561 cagctactcg ctgctgccgg cgttcaccga gccctatcgc aagggctggc agctcgcggt
     7621 cgaggaaatc aatgccgccg gcggcatcaa cggcaagaag ctcgtcgtcg tctccaagga
     7681 cgacggcggc aagcccgccg acgcccagac cgccgccaac gaactggtat cgagcgagaa
     7741 cgtggcgatg ctcgccggcg cgttcctgtc caatatcgga ctggccgtca gcgatttcgc
     7801 taaccagaaa aaagtcttct tcctggccgc cgagcctctg accgacgcca tcacctgggc
     7861 caagggcaat cgctacactt tccgcctgcg tccttcgaat tacatgcagg cggcgatgct
     7921 ggtggaggaa gcggcaaagc tgccagccaa gcgctgggcg acaatcgcgc cgaattatga
     7981 atatgggcaa tcggcggtcg cggtgttcaa gaagctgatg tcggaaaagc ggccggatat
     8041 ccagtgggtc gacgagcagt ggccgccgca gggcaagatc gacgcgggtc ccgtcgtgca
     8101 ggcggtcgcc gccgccaacc cggaagccat cctcaacgtt accttcggcg ccgatctcat
     8161 caaactcgtg cgcgaaggca acacccgcgg cctgttcaag gatcgcgcgg tggtgagttt
     8221 cctcaccggc gagccggaat atctcgaccc gctgaaggaa gaaacgcccg aaggctggat
     8281 cgtgacggga tatccctggt acagcatcaa gacaccggag cacgacgcgt tcatcaaggc
     8341 ctatcaggcc aagtacaatg actatccgag gctcggctcg atcgtcggct atcagaccat
     8401 caaatcggcc gcggccatcc tcgccaaggc caattcgacc gatccggaga agctaatcgc
     8461 ggcggcggag ggattgtcgg tgccgtctcc gttcggtgag attaccttcc gcaagatcga
     8521 tcatcaatcg acgctcggcg ccttcgtcgg caagaccgcg ctgaggggcg gcaagggcat
     8581 catggtggat tcggtctatc gcaagggctc cgactatctg cccagcgacg ccgaagtcgc
     8641 aaagctgcgc ccaaaagagt gagtctgtcg gctgcggggc gcgcaagcgc ctgcagcatt
     8701 ttgagcgcgg accgccaatg gcattttatt tcgttcagtt cctgaccggc cttgccagcg
     8761 cggcgtcgct gtttctcgtc gcgtccggcc tgtcgatcat tttcggcgtc acgcgaatcg
     8821 tcaatttcgc gcatggcgca ttctatatgc tcggcgccta tgtggcgttc acgctgaccg
     8881 aacgattctc cggcgccatc ggcttttggg gcggcattgt cgtcgctgcg ctgatcgtgg
     8941 cggcggtcgg cgttctcatc gagatggtgc tgctgcggcg catctatcat gcgccggaac
     9001 tgttccagct ccttgcgacg ttcggcctga ccctgatggt cgaggatctg gtggtgctga
     9061 tctggggacc gagcgacctg ttgggtcgtc gtgcgccggg atttaagggc gcggttgatt
     9121 tcttcggcca gaacgttccg acctatgacc tgttcctgat cggactgagc ccggtcgtgc
     9181 ttggaatctt gtggctgcta tttcagcgaa cccgctgggg cgtgctggtt cgtgccgcca
     9241 cgcaggatcg cgacatggtg gcagcgctcg gcgtcaatca aaaatggctg ttcaccagcg
     9301 tattcgcggt cggggttttc ctcgcggcgt tcggtggcgc gctgcagatt ccgcgcgatg
     9361 cggtgcatca cgcgctggat ttgcggatta tcgtcgacgt gttcgtggtg gtggtgatcg
     9421 gcggtctcgg cagcatcatc ggcgcctttg tcgccgcggt gctggtgtcg gagttgaatg
     9481 cattcggcat cctgattttc ccgaagatct ccatcatcct ggtgttcctg gtgatggccg
     9541 cggtgctgat cgtgcggcca tggggactgt tcggaaagcc ggaagcccgc gcccgtcgca
     9601 cgcccgggct gaccgtcaat ccgtggcggc cgctgacgtc gaatgagcgc cttgcggcga
     9661 tggccgcgct ggtactggcc gcggcgctac cgctgatcgg cggcaattac gcgctcacgg
     9721 tcggttcgga gattgcgatt ttcgtgatct ttgccaccag cctgcatttc ctgatgtcgg
     9781 tcggcggcct tgcctcgttc ggccacgcgg cgtatttcgg actcggcgcc tatggtgttg
     9841 cgttgctggc caagctcgcc ggcttgccga tgatcttgtc acttttgctc ggtccgctgc
     9901 tggggtgttt gggcgcggcc gtgttcgggt tcttcgcggt tcaactgtca ggcgtgtatt
     9961 tcgccatgct gacgctggca ttcgcgcaga tcgtctggtc gattgcgttc cagtgggtcg
    10021 aagtgaccgg cggcgacaac ggcatcctcg gcgtctggcc cgagacatgg gcggcggcgc
    10081 cggcgcattt ctactggctt tcgattggcg tcgccgcgct tgcggtcgca gctcttcgga
    10141 tcatcgtatt ctcgccgttc ggctatgcgc tgcgggcgac acgcgactcg ccgttgcgca
    10201 gcgaggccat cggcatcgac ggcaagcgca tccagtggac cgccttcgtg attgccggca
    10261 ccgttgccgg cattgccggg gcgctcttcg cctatctgaa aggcagcgtc tttcccgaca
    10321 gcctcggtat ctcgctctcg gtcgatgcgc ttgtaatggt gctgctcggc ggcgtcgaga
    10381 cggtttccgg cgccgtcgtc ggcgccatcg tctacaaggc gctctccatc tggctggtga
    10441 gccagaccga tctgtccaag ctggtgctcg gcggcgtcat cgttctgatc gtggtggcgt
    10501 ttcccaaagg catcgtcggg atgctggaaa cgatccggaa tcgtcgccga ggtgaagcgg
    10561 ccaagcctga atcgtccaag cccgcactcg cgcccgggat cgagaccgcc gaatgagcat
    10621 cgcgccaaca ctgctttcag tcgagggctt gagcaaatcc tatggcggcg tccatgcggt
    10681 gcgcaacgtg tcgttcgcgc tcaaggccgg cgagatcctg gcgctgatcg gtcccaacgg
    10741 cgctggaaaa agcacatgct tcgatatgct caacggccaa aatattccag atagcggccg
    10801 gatcagcctg ctcggccagg acaccaccgg caagaaacca cgcgcgatct ggcggctggg
    10861 cgtcggacgc accttccaga tcaccgcaac atttccgacc atgaccgtgc gcgagaacgt
    10921 tcaggtcgcg ctggtgtcgt atcgccggca gttgttcaat ctccgggcct cgacgcagtg
    10981 gttcgcaaag caggaggccg gtcgcctgct ggaactggtc gggatgagcg gatatgcgga
    11041 gcgtccgtgc ggcgaactcg cctatggcga tctcaagcgg ctggaactcg cgatcgcgct
    11101 cgccaaccag ccaaaactgc tgctgatgga tgagccgacc gccggcatgg cgccgcggga
    11161 gcgcatcgag ctgatgcggc tcaccgcgca aatcgcgcgg cagcagtcga tcggcgtgct
    11221 cttcaccgag catgacatgg acgtggtgtt cgagcacgcc gaccggatcc tggtgctcaa
    11281 ccgcggcacc ttgattgccg aaggcacgcc tgaagaagtc cggcgcaatc cgcaggtccg
    11341 cgccatctat ctcggcgaag gcctggtcta tgatatcagc caccgcgagg gagcgccgtc
    11401 atgaagctct cggttcagga cctcaacagt cattacgggc cggcgcacat tctgttcgac
    11461 atcgcacttg aggtcgacga cggcgaggtg gtggcgctgc tcggacgcaa tggcgccggc
    11521 aagtccacga ccttccgctc catcgtcggt ctggtcgagc aacgcaccgg ccgcatcata
    11581 ttcgaaggca aggacgtttc ggatcagccg acccatgcga tcgtgcgcgg cgggctcggc
    11641 tatgtcccgg aagagcggcg cattttcacg gatctgacgg tcgatgaaaa cctggaagtg
    11701 ggccgccagc cgatcaggcc gaacgcgccg cactggaccc gggagaaatt atttgcactg
    11761 tttccgaacc ttggcgagat gcgcggccgc ccgggcgggc ggatgagcgg cggtgagcag
    11821 cagatgctga ccatcgcgcg gaccttgatg ggaaacccgt cgctggtgct gctcgacgaa
    11881 ccgtcggagg ggctgtcgcc gaagatcgtg gagcagatgg tcgacgccat cctgaccatg
    11941 aagaaggaag gcgtcagcat tgtcgtgtcc gagcagaatt tacatttcgc gagattgatc
    12001 tccgaccgcg cctatatcat cgagcgtggc cgcatgtgct tcagcggcac catgagcgag
    12061 cttgacgccc gcccggacgt tcgcgacgcg catctggcgt tgtgagcgcc ggcaggaaaa
    12121 agggctggga taacgatatg accaggagtg tttcgccaaa acggagcatg aaaccttcgc
    12181 ggccgaatta cgtccttgag gagcaggtcg gttttatcct gcgtcaggta tggcagcgcc
    12241 acgcggcgat cttcgcgcgc gagatcggca tcaatctgac gccgacgcaa tgggcggcgc
    12301 tcgcaaaact caccgagacc ggaccatgct cgcagaatca gctcgggcgg ctgacggcga
    12361 tggatgtcgc caccatcaag ggcgtgatcg atcggctcag cgcccgcggc ctgaccgaga
    12421 ccagccccga tccggaagat ggccgtcgcc tcctggtcag cctcacgcgc gccggtcagc
    12481 agatggccga aaaggccgcg cccagcgcgc tcgcgatcac cagggaaacg cttgcgccgc
    12541 ttgatcccaa ggagcgcgaa acgctgatcg cgctgttgag caagttgcgg tgacgatggt
    12601 ggggatcaag ccaaaatcgt cttcggcttg aacggccggc agccgcccgt tacggtgccc
    12661 cggcaattcc gggagaagca aaaaatgaga caggtcaggg tcggcgacat caccatcgat
    12721 gcggtgattg aacgtgaggg cccagggcca gttccgcggc gcgcgtggtc ggaaggctgg
    12781 gctgaacgat cacggcatcg acgccgtcct tgttcatgcg cggaaaggac gtttcgagtt
    12841 cgtcgttacc gttgaccatg atccggtcta actcgaatcc tgtccccgac gcggcgccct
    12901 cgatttgcgc aagaaacggc tttgtaaaca aatcggcagt attgcataga acggccagtc
    12961 gcttcgccga cggcagcatt tcacggatga gttcaacgtt ttttccggct agttcggccg
    13021 tcacccccgc catgccggta atgttgccgc ccggtcgaac gaggctggca accactccgg
    13081 tcgcaaccgg atcgcccgcg cccgccatga cgatcggaat ttggcttgtc gcttgtttgg
    13141 ccgcgcgcac cgcaggagtt agccacgtga cgatgacatc ggcctttgca ccgaccagtt
    13201 cagcagcgag tcccggcagg gcatccattt tacccttcgc ggtccgaaac tcgaagcaaa
    13261 gattctgccc ttccagatat ccgagacggc gcagctcttc tttgaaaaca ctcaggaagg
    13321 gcgcgggatc gagatttccg gggacgagga ttccgattgt gaccgttttc ggctgttgcg
    13381 cacaggcggc cagcggccaa aatgcagcaa cgccaagcgc cgcgatgaac ttccgccgtt
    13441 tcacatgacc tcgctgagca ccttcatctt ccgatggttt gatcataaca tcttcatgcc
    13501 gggccggcta cgtgaggacc ggcgccgaaa cacccttcca catatcatcc ccatggcata
    13561 agagcgggcc gcaagttttt ttcagggctc gtggtgcaca tgattcgtct cgataacgtc
    13621 agcaagcaga taggccacca gattctcttc atcgaggcct ccgcggcgct ccagaagggc
    13681 gagaaggtcg gcctcgtcgg ccccaacgga gccggcaaga ccacgctgtt ccggatgatc
    13741 accggcgagg agagccctga cgagggccag gtggccatcg atcggggcgt caccatcggc
    13801 tacttcagcc aggacgtcgg tgagatgtcg ggccgaagtg cggtgtccga ggtgatggac
    13861 ggcgccggcc ctgtcagtgc cgtggcgagc gaactgaaag agctcgaagc tgcgatggga
    13921 gatccggacc gggcggatga tatggacgat atcatcgcgc gttatggcga ggtgcaggct
    13981 cgcttcgagg aactggacgg ctacgccctg gaggggcggg cacgcgaagt cctcgccggc
    14041 ctgagtttca gccaggagat gatggacggt gacgtcggcg cgttgtcggg tggctggaag
    14101 atgcgggtgg cgctggcgcg catccttctc atgcgcccgg acgccatgct gctcgacgag
    14161 ccgagcaacc atctcgacct cgagagcctg atctggctcg agcagttcct gaaaggttat
    14221 gagggcgcgc tgctgatgac ctcgcacgac cgcgagttca tgaatcgcat cattggcaag
    14281 gtgatcgaga tcgacggcgg tcagcttacg acctattcgg gcgattacga gttctacgag
    14341 cagcaacgca cgctgaacga gaagcagcag caggcccagt tcgagcgtca gcaggccatg
    14401 ctcgccaagg aaatcaactt tatcgagcgt ttcaaggcgc gcgcttcaca cgccgcccag
    14461 gtgcagagcc gggtgaagaa gctggagaag atcgaacggg tggagccgcc caggcgccgc
    14521 cagacggtat ctttcgactt catgccggcg ccgcgctcgg gtgaggacgt ggtgagcctg
    14581 aagaacgtgc ataagggcta tggcgtcagg cgcatctacc aggggctgga ttttatggtg
    14641 agccggcggg agcgctggtg tgtgatgggc atcaacggcg ccggcaagtc aacgctgttg
    14701 aagctggtgg ccggctccac gcaaccggat gatggtacgg tggcgctggg tggcagcgtg
    14761 aagatgggtt acttcgcgca gcacgccatg gatctgctgg acggcgagcg cacggtgttc
    14821 cagtcgctgg aggattcgtt tccgcaagca ggacaaggct cgctacgcgc gctggccggc
    14881 tgcttcgggt tctccggcga cgatgtcgag aagaaatgcc gggtgttgtc gggcggtgag
    14941 aaagcccggc tggtgatggc gatgatgctc tacgacccgc caaacttcct ggtgctggac
    15001 gagccaccaa ccatctggac atggcgacca aggagatgct catcacggcg ttgtcgcagt
    15061 acgagggcac catgctgttc gtctcgcatg accggcactt cctggccgcg ctgtccaatc
    15121 gggtgctgga actgaccccc gagggcattc accagtacgg cggcggctat acggagtatg
    15181 tcgcgcgcac cgggcaggag gctcccgggc tgcgcagctg agccgggaac aaggcggaag
    15241 ccgcgcccga tcaacaagcc atcgtctgtc cgccgccaca cggcgtcgca cacgacgatg
    15301 tcgagccatg aggccacgcc ttcttgcgtt cttgcggcgt gaggtcgacg ttctgttctt
    15361 agggaagatc cggggacagg ctgccgtgct gcgctttgtg gatcgacgac ggaacaattc
    15421 cgaaatattt ccggaagaca cggctgaaat gcgacgagct tgaaaagccc cacgaaaatg
    15481 caacatccgt gattgttttt ccgccctggg cctccagctc ctggcggcag ttctgcaacc
    15541 ttgcctgcca gatgtaatcg ctgacggtca tgcccctgtc gctgaacaac atgtgcagat
    15601 agcgtttggt gcagcccaat gccgccgaaa tctggtcgat gctgagatcc gggtcgcgca
    15661 ggtgttcgcg gatgaaggcc tgggcgcgca catacatcgc caccggacca ctgcagtcga
    15721 gcggcgttcc ggcctgccga agaggcagca gcaggagatc gatgagcgaa tcggcgacgc
    15781 cgatggcgtt gtatggggac agtttttcag cttcgccaaa ggcggcatgc acgaaatcat
    15841 aggcgatccg gccggtgccg gtgcgcgccg aaagcttgca tggcgacatc ttcgcagaat
    15901 gaaagccgcg ttctcgcaac aattcctttg gcacgatgac gacttcgtgg cgcgtcagcg
    15961 atggactgat gatcgtatgc ggacaggata cgtcataggc aagacagtca ccgggcacca
    16021 gttcgatgcg gcggccgctc tgctcgaaat gagaaattcc atatgtctga aacagtattt
    16081 tgacgtaggg atgttcgcct gattttgccc gcgagacggg atgcgcaagc cgatgctggc
    16141 tcgcctcgat ctggcagagc tttagccgcg aaacggtcgt atagtttatc tgcccttcga
    16201 gcgacgaggc ttcgagcgga tcaacatcaa attgtccgca aagctccgtc agcgcatcgg
    16261 tccagcactg gatctgtttt tttggcgaca atccggaggt gctaagtgaa cggacggtat
    16321 gggacattat ccagccactg atttggattt gggatcaaat gtaactgctc ggcgcgagag
    16381 gcatcgggtc acgatgaagc gacatttacc tcaatttgaa agcggtcgtt ggaacccgaa
    16441 gcagcgctcc tcctgacatc catttgatga tcctccgatt tagtttcggc gtcgtcaagg
    16501 ggaacctgaa agtcgtctcg cgcaggcacg gttcggagcg cgctgcacaa ggaccgatgc
    16561 tacgaaggtt gaaggcagcg ccgcccaaaa cactggccgg cagctaaaat gttcgttgcg
    16621 tttcgctttc gagcaaaccc gcttccctct tgggcaagtt tcctgtcgcc gaaccggata
    16681 ggactacgca ggaaaataga gcgagtggac cgtttcgacg gaaacccagg gctcagatct
    16741 ggaggaagaa accgtcgcag cagcatcgct acgtcgctca atccgagatg cgctgtcgga
    16801 acaaacatcc ccgctgcaag aactgcgctg ggcgtgtgcc caccgcacaa accaaataaa
    16861 accgaaacaa catgggaggc tttgtgatgc gcaaggtgct atttgcaacc tgttttggcg
    16921 ctgtggcgac attcgccgcg ggtgccgcta ctgccaatga tgaactgggc aagatgtcgc
    16981 agaacccgaa ggattgggta atgccggcgg gcagctacga caatcagcga tattcgaagc
    17041 tcaatcagat caatgccgcc aatgtcggca agctacaggt ggcatggacg ttctcgaccg
    17101 gcgtgctgcg cggtcatgaa ggcggaccgc tgatcgtcgg caacatgatg tacgtccaca
    17161 cgccgttccc aaacaaggtc tatgctcttg acctttccga cgagaacaag atcgtctgga
    17221 agtatgagcc caagcaggat ccaaacgtca tcccggtgat gtgctgcgat acggtcaacc
    17281 gcggcgtggc ttacggggac ggcaagatct tcctgcacca ggccgacacc acgctggtgg
    17341 cgctcgacgc caagaccggc aaggtggagt ggacggccaa gaac
//
LOCUS       DATNOI010000665         9781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1682048,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000665 DATNOI010000000
VERSION     DATNOI010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9781
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682048"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..410
                     /locus_tag="VN968_26735"
                     /pseudo
     CDS             1..410
                     /locus_tag="VN968_26735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496853.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            581..1564
                     /gene="hpaD"
                     /locus_tag="VN968_26740"
     CDS             581..1564
                     /gene="hpaD"
                     /locus_tag="VN968_26740"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HXL32862.1"
                     /translation="MPVPQHTFDPPFNIVRSSHVVLDVVDLNASREFYETTVGLHVED
                     RDDRAVYLRGSEEHQHHSLVLRKAPAAACNRLGFKVGNETDLDKAAAFFSENGIAYAF
                     ADQPFQGRTLQFTDPFGFQIELHATMEKRPHLLRRYDLYKGCHPQRLDHFNVFAAEVQ
                     DTVDFYARLGFRLTEYGEEDGPNGRIAAAWMHRKGNVHDFAITNGRGPRLHHFAYWVP
                     TAMNILHLCDVMASSGLLKNLERGPGRHGISNAFFLYIRDPDGHRLELYTSDYFTGDH
                     DHEPMRWSLRDPRRQTLWGAPAPRSWFEQGSPFSGQAIREPLFVADVTIAD"
     gene            1813..2523
                     /gene="modA"
                     /locus_tag="VN968_26745"
     CDS             1813..2523
                     /gene="modA"
                     /locus_tag="VN968_26745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HXL32863.1"
                     /translation="MSPIENLEAAELKLIAGGSMTGALNELGPQFERASGHKLVIHFN
                     STPNIIALVTSGTPFDLAVVPVDVFKDAGAKARFAPSPTIDIARVGYGVIVRAGTPKP
                     DISTPDALKQTLLDAPSVAFVPASAAGAYVLKVFDRLGIAEAMKAKTKPQTTPADIAP
                     AVAKGDAELGVFLVNVLMAPGVEPAGSFPAELQQDLVFTAAVAADTKEADAAKAFIDY
                     LTTPAATAVIRAKGMTPG"
     gene            2579..3451
                     /locus_tag="VN968_26750"
     CDS             2579..3451
                     /locus_tag="VN968_26750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXL32864.1"
                     /translation="MTSHCLATYSVDGTTKYGAVVEGGLVDLSARFAKDYPTLREAIA
                     AGMLTRLAEDAAQRSPDHALDAITWLPPIPSPEKIICIGVNYPDRNAEYKDGQDAPKY
                     PSMFMRTPRSFVGHDTPLVRPRASAQLDYEGEVVLIIGKAGRHIPESTALDHIAAVTL
                     CNEGTIRDWVRHAKFNVTQGKNFDSTGSLGPWLVPYTGESQIADIGLTTRVNGETRQD
                     DRTGRLIFGFRYLINYISTFTTLVAGDVIVTGTPTGAGARFDPPRYLKPGDVVEVEAE
                     NIGVLRNGVVDEAS"
     gene            3480..5105
                     /locus_tag="VN968_26755"
     CDS             3480..5105
                     /locus_tag="VN968_26755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXL32865.1"
                     /translation="MTVCTGGEAIVSGLVAHGVDTVFGLPGAQIYGLFDAFQQAQLKV
                     IGVRHEQACGYMAYGYARSTGRPGVFSVVPGPGVLNAGAALLTAFGSNEPVLCLTGQV
                     PTQYLGKGRGHLHEMPDQLATLRSFVKWADRIEYPDAAPTLVSRAFQEMLSGRRGPVA
                     LEMPWDVFTQRAETGPAKPFEPFPAPQPDTERIKAAAALIANSKTPMIFVGSGAIHAR
                     DEILELAELIDAPVVAFRSGRGIVSNAHELGLTMAAAYRMWPHTDLMIGIGSRMELPT
                     MFRWPFRPDGLKSIRIDIDASEMRRFMPDAPVIADARAGTRELLAAVRKSGYTKTSGR
                     RDAIRDASATALREIQKVQPQMAYLNVLREVLPPNAIVTDELSQVGFASWYGFPVYEP
                     RTFITSGYQGTLGSGFPTALGAKVAHPDRPVVAITGDGGFMFGVQELATAVQFNIGVV
                     TLVFNNNAYGNVRRDQRERFDGRVVAADLVNPDFVKLAESFGVGAARVTSPGTFRPAL
                     EKALADGGPYLIDIEVPRDSEVSPWTFIHPAKP"
     gene            complement(5213..5866)
                     /gene="alkB"
                     /locus_tag="VN968_26760"
     CDS             complement(5213..5866)
                     /gene="alkB"
                     /locus_tag="VN968_26760"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HXL32866.1"
                     /translation="MTVDLFESVPDVRPSQEAMAERAVLLRGCAKPFEGDLIADLRDI
                     VTQAPFRHMVTPGGHQMSVAMTNCGSVGWVTDRTGYRYDGIDPESGKPWPAMPPSFRR
                     LAGQAAAQAGFDGFCPEACLINRYQPGARMSLHQDKDETDFAAPIVSVSLGLPAIFLF
                     GGLKRSDRPRRFRVEHGDIAVWGGPTRLAFHGVAPLADGEHAVMGRQRINLTFRKVR"
     gene            complement(5876..6625)
                     /locus_tag="VN968_26765"
     CDS             complement(5876..6625)
                     /locus_tag="VN968_26765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HXL32867.1"
                     /translation="MKSTAKNIIHPAPASDIAARVDAIDWTQAGTDLDAQGCAVLKGL
                     LSVEECRALAAVYPDDSRFRSRVVMGRHGFGRGEYKYFSYPLPDLIAELRPALYAQLH
                     DVANSWNEAMGIDIRYPRSHDAFLKRCHAAGQTRPTPLLLQYGVGDYNCLHQDLYGEH
                     VFPLQVAILLSEPGRDFTGGEFVLTEQRPRMQSRPEVVPLAQGDAVAFAVHVRPMQGT
                     RGFYRVNLRHGVSRIRSGHRHTVGVIFHDAK"
     gene            complement(6823..7509)
                     /locus_tag="VN968_26770"
     CDS             complement(6823..7509)
                     /locus_tag="VN968_26770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HXL32868.1"
                     /translation="MFDLTFTIDVQGTTTPLTLAIDQAVIAGWTGRDPVARDKHIAEL
                     EAIGIARPASTPIYYRVAARRLTTEDRIEVSGAESSGEVEFVLIGWQGRIFVGAASDH
                     TDRKVEAYSVTVSKQMCDKPIAPVLWELEEVVGHWDQMILRSYAWIDGARVLYQEGTL
                     DSMLPVAELIERGFGGAGFPDGCAMFGGTFAAKGGIRPASRFEFELEDPVLKRSIRHG
                     YDVIELPVLG"
     gene            complement(7502..8851)
                     /locus_tag="VN968_26775"
     CDS             complement(7502..8851)
                     /locus_tag="VN968_26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXL32869.1"
                     /translation="MPNTPTLATLADDLEGGRTSARKLVDDCLARIADTSGEGMRTFI
                     HVDAEAAIEAAEAMDRLREVKAAPSRYAGIPVSIKDLFDIKGQVTRAGSRALEDAAPA
                     EADASVVARLRRAGFVVIGRTNMTEFAFSGIGINPHFDTPKGAWRRHVGHVPGGSSSG
                     AAVSVVDGMAHGALGTDTGGSCRIPAAFNGIVGFKPTQRRVPLEGGVPLSSTLDSFGP
                     LARTVGCCAVLDAVLAGEPVKPLQPRPIKGMRLAVPTTVVLDQLEDAVARTFERALET
                     LSRQGALIERIEVPEFLDVGMMNAKGGFAAAESYAWHRYLIVSHGDVYDPRVRTRILR
                     GEAISAADYIDLLGARRSLIARTEKRIAPHDALILPTTANTPPRIADLADDKAFTAQN
                     LLALRNCTLINMIDGCAISLPCHRQGEVPVGLMLAGAGGSDRRIFELAAGIEDAIRV"
     gene            complement(8882..9202)
                     /locus_tag="VN968_26780"
     CDS             complement(8882..9202)
                     /locus_tag="VN968_26780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32870.1"
                     /translation="MKLREIAHSRTGDKGNTSNISVIAYHEKHYPLLLAQVTSARVKA
                     HFAGVVEGEVVRYELPNIAALNFVMAGALGGGVTRSLALDAHGKSLSSALLDLEIDAV
                     SDNE"
     gene            complement(9206..>9781)
                     /locus_tag="VN968_26785"
     CDS             complement(9206..>9781)
                     /locus_tag="VN968_26785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HXL32871.1"
                     /translation="AQTCKEQLLYEVHDPKRYIQPDVVADFSQVKVEEIATDRVRISG
                     GRGTGRTDTLKVSVGYVDSFIGEGQISYAGPGALARGRLALDIVRERLKLTDVASSEL
                     RFELIGVDSLHGTEISARANEPYEVRVRVVGRTENLREAIRIGNEVETLYTNGPAAGG
                     GAFKSARDVVAVASVLLPRELAKPIIRFVGA"
BASE COUNT         1823 a         3234 c         2988 g         1736 t
ORIGIN      
        1 aagacgcatg acggacccgg cggcgggcac tatgtgcagc cgacgctggt caccggcgcg
       61 catgccaaga tgcgcgtcgc gcaggaagag gtgttcggcc ccttcctgac ggttattccg
      121 ttccgcgacc aggatgacgc catcgaaatt gcgaattccg ttcagtatgg cctcaccggc
      181 tatgtctgga ccgcggacat gggccgcgcg ctgcgggtcg cggatgcgct ggaggccggc
      241 atgatctggt tgaattccga aaacgtccgc catctgccga cgcccttggc ggcatgaaat
      301 cgtcaggcat cggccgcgac ggcggcgatt attcattcga cttctacatg gaaaccaagc
      361 acgtttcgct ggcgcgagga acgcacaaga ttcagcggct tgggatataa attgaactcg
      421 tcattccggg gcgcgcaaag cgcgagcccg gaatccataa ccacgacagg gcgtatggat
      481 tccgggcctg cgccaagagg cgcatccacg atgtgcaatt gcacatcggg ggaatgacga
      541 aagataagat tttccgctca cgcgactaca ggaaacgttc atgcccgtcc cgcagcacac
      601 cttcgatccg ccattcaaca tcgtcaggag cagccacgtc gtgctcgacg tggtggacct
      661 gaacgccagc cgggagtttt acgaaaccac cgtcgggctg catgtcgagg atcgcgacga
      721 ccgcgcggtg tacctgcgcg gcagcgagga gcatcagcat cactcgctgg tgttgcgcaa
      781 ggcgcccgcc gccgcctgca accgcctggg cttcaaggtc ggcaatgaaa ccgatctcga
      841 caaggcggcg gcgttctttt ccgagaacgg catcgcttac gcttttgcgg atcagccgtt
      901 ccagggtcgc acgctgcaat tcaccgaccc cttcggcttt cagatcgagc tccatgcgac
      961 catggagaag cgtccgcatc tgctgcgccg ctacgatctc tacaaaggct gccacccgca
     1021 gcggctcgac cacttcaacg tgttcgccgc ggaggttcag gacaccgtcg acttctacgc
     1081 ccggctgggt tttcggctga ccgaatatgg cgaggaagac ggcccgaacg ggcgtatcgc
     1141 tgcagcgtgg atgcatcgca agggcaacgt gcacgatttc gccatcacca acggccgtgg
     1201 ccctcgcctg caccattttg cctactgggt gccgaccgcg atgaacattt tgcatctgtg
     1261 cgacgtgatg gcctcgagtg gcttgctcaa aaaccttgag cgcgggcccg gccgccacgg
     1321 catttcaaat gcgttctttc tctatatccg cgaccccgac ggccatcgcc tggaactcta
     1381 caccagcgat tactttaccg gagatcacga ccacgagccg atgcgctggt cgctcaggga
     1441 tccacgccgg caaaccttat ggggcgcgcc cgcgccgcgc tcgtggttcg agcagggctc
     1501 gccgttttcg ggacaggcga tacgcgaacc gctgtttgtc gccgacgtta cgattgccga
     1561 ttagggtcga tcgatgaatt ctcgccgcaa tatgcgattt tccgatagcg aaggttctcg
     1621 ccgcgcgtcg tcccggcgcg cgccgggacc cataacccgc ggcggccatg ttgcagaagg
     1681 tgtctccccg agtgccccaa tgatagaaca cggggtatgg atgccggcgt tcgccgggac
     1741 gacgtaaatt gtatcggacc cccgatgact tctctcgtgg cagtcacttt gatactgagc
     1801 ttgggtcttg tcatgtcacc gattgaaaac ctggaagccg ctgaactcaa gctcatagcc
     1861 ggtggctcga tgaccggcgc gctgaatgaa ctcggccctc agttcgagcg cgcctccggc
     1921 cacaagcttg tcattcattt caattccacc ccgaacatca tcgcgctggt tacatcgggc
     1981 acgccgttcg acctcgcggt cgttcccgtc gatgttttca aggacgccgg cgcgaaggcc
     2041 cgcttcgccc cctcgcctac catcgatatc gcgcgggtcg gttacggcgt catcgtccgc
     2101 gccgggacgc ccaagcccga catctctacg ccggacgcct tgaagcagac cctgctcgat
     2161 gcgccgtcgg ttgccttcgt tccggcaagt gctgccggcg cttatgtcct gaaggtgttc
     2221 gaccgtctcg gcattgccga ggcgatgaaa gccaagacca agcctcagac cacaccagct
     2281 gacatcgctc cggccgttgc caagggcgac gccgagctcg gggtgtttct cgtcaatgtg
     2341 ttgatggccc ccggtgtcga gcccgccggt tcattccccg ccgaattgca gcaggatctg
     2401 gttttcacgg cagccgtcgc ggcggatacc aaggaagcgg acgccgccaa ggcgttcatc
     2461 gactatctga ccactcccgc cgccacagct gtcatcaggg ccaaaggcat gacgcccggc
     2521 tgaagcggcc ctgaacgccg ggcgacagct ttacgaacca cctcttattg gacatctcat
     2581 gacctcacat tgcctcgcta cctactccgt cgacggcaca accaaatacg gcgccgtggt
     2641 cgaaggcggg ctggtcgatc tttccgcgcg ctttgcaaaa gactatccga cgctccgtga
     2701 agcgattgcg gccggcatgc tgacaagact ggccgaggat gccgcgcagc gttcgccgga
     2761 ccacgcgctc gacgcgatca cctggttgcc acccatcccc tcgccggaaa aaatcatctg
     2821 catcggcgtg aattatccgg atcgcaacgc ggaatataag gacggccagg acgccccgaa
     2881 atatcccagc atgttcatgc gcacgccgcg ctcgttcgtc ggccacgaca cgccgctggt
     2941 tcgtccgcgc gcatccgcgc aactcgacta tgagggcgaa gtggttttga tcatcggcaa
     3001 ggccggccgg cacatcccgg agagcacggc gctcgatcac atcgccgccg tcacgctgtg
     3061 caacgaaggc accatccgcg actgggtccg gcacgcgaaa ttcaatgtga cgcaaggcaa
     3121 gaatttcgat tcgaccggca gcctcggccc gtggctggtt ccctacaccg gtgaatcgca
     3181 gatcgcggat atcggactga ccacgcgggt caacggcgag acgcggcagg acgaccgcac
     3241 cggccggctg atcttcggct ttcgctatct catcaactac atctcgacct tcaccacgct
     3301 ggtagcaggc gacgtcattg tgacgggaac gccgaccggc gccggcgcgc ggttcgaccc
     3361 gccgcgctat ctgaagcccg gcgatgtcgt cgaagtggag gccgaaaaca tcggcgtgct
     3421 gcgcaacggc gtggtcgacg aagcctcgtg atcccctttg caagcgattg gataatgcaa
     3481 tgacagtgtg taccggcggc gaagccatcg tcagcggcct cgtcgcgcat ggcgtcgata
     3541 ccgtgttcgg cctgcccggc gcccagatct acgggctgtt cgacgccttc cagcaggcac
     3601 agctgaaggt gatcggcgtc cgtcacgagc aggcctgcgg ctacatggcc tatggctatg
     3661 cgcgatccac tggcaggccc ggcgtgttca gcgtggtgcc cggcccgggc gtgctcaatg
     3721 ccggtgctgc cttgctgacc gccttcggct ccaacgaacc ggtgctctgc ctgaccggac
     3781 aggtgccgac gcaatatctc ggcaagggcc gcggccatct gcacgaaatg ccggatcagc
     3841 tcgcgacact tcgaagtttc gtgaaatggg ccgaccgcat cgaatatccc gatgccgcgc
     3901 cgacactcgt cagccgcgcg ttccaggaaa tgctgtcggg acggcgcggc cccgtggcgc
     3961 tggaaatgcc gtgggatgtg ttcacgcagc gcgccgaaac cggcccggca aaaccgttcg
     4021 agccttttcc ggcgccgcag cccgacaccg aacgcatcaa ggccgccgcc gcgctgatcg
     4081 cgaacagcaa gacgccgatg atttttgtcg gcagcggcgc cattcacgcc cgcgacgaaa
     4141 tcctcgaact ggcggaattg atcgacgcgc cggtggtggc attccgcagc ggccgcggca
     4201 tcgtcagcaa tgcgcacgaa ctcggactga ccatggcggc ggcctatcgg atgtggccgc
     4261 acaccgatct gatgatcggg atcggttcgc gcatggaatt gccgaccatg ttccgctggc
     4321 cgttccgccc cgatggcttg aaatcgatcc gtatcgatat cgatgcttcg gaaatgcgcc
     4381 ggttcatgcc ggatgctcct gtcattgccg atgcgcgggc cggcacgcgc gaattgctcg
     4441 cagcggtgag aaagtccggc tacaccaaaa ccagcggccg ccgcgacgcg atccgcgacg
     4501 cctcggccac agcgctgcgc gagatccaga aggttcagcc gcagatggcc tacctgaacg
     4561 ttctgcgcga ggtattgccg ccaaacgcca tcgtcaccga cgaattgtcg caggtcggct
     4621 ttgcctcctg gtatggcttt ccggtctacg agccgcgcac cttcatcaca tcaggctatc
     4681 agggcacgct cggctccggc tttcccaccg cgctcggcgc caaggtggcg catccggacc
     4741 ggccggtggt cgccatcacc ggcgatggcg gcttcatgtt cggcgtgcag gaactggcca
     4801 cggccgtgca gttcaacatc ggcgtggtca cgctggtgtt caacaacaac gcctatggca
     4861 atgtaaggcg cgatcagcgc gagcgttttg acggccgcgt ggtggcggcc gatctggtca
     4921 atccggattt tgtgaaactc gcggagtcct ttggcgtcgg tgcggcgcgc gtgacctcgc
     4981 ccgggacgtt tcggcccgcg ctggaaaagg cgctggcgga tggcgggccc tacctcatcg
     5041 acatcgaagt gcccagggat tccgaggtta gtccgtggac gttcattcat ccggcgaagc
     5101 cgtaagctac acactgtcat tccggggcga cgcgaagcgt cgaaccagga atctcgagat
     5161 tccgggttcg cgctacgcgc gccccggaat gacatagcaa attcggcagc ccctatcgca
     5221 ccttccggaa cgtcaggttg atacgctgcc ggcccatcac agcatgctcg ccgtcggcga
     5281 gtggtgcgac gccgtgaaac gccagccgcg tcgggccgcc ccacaccgcg atgtcgccgt
     5341 gctcgacccg aaaccgccgc ggccggtcgc tgcgcttcaa tccgccgaac agaaagatcg
     5401 cgggcaaccc cagcgagacc gagacgatcg gcgcggcaaa atcggtctcg tccttgtcct
     5461 ggtgcagcga catgcgcgcg cccggctggt agcggttgat caggcacgcc tccgggcaaa
     5521 acccgtcaaa acccgcctgc gccgccgcct gcccggcaag cctgcgaaac gatggcggca
     5581 ttgccggcca gggttttccg gactccggat cgatgccgtc gtagcgataa ccggtgcgat
     5641 cggtgaccca gccaacactc ccacaatttg tcatcgccac cgacatctga tggccacccg
     5701 gcgtcaccat gtggcgaaac ggggcttgcg tcacgatatc ccgcaagtca gcgatcagat
     5761 cgccctcgaa cggttttgcg catccgcgca gcagcacggc gcgctccgcc attgcctcct
     5821 gcgacggacg cacatccggc acgctctcaa ataaatccac tgtcaaaact cgcactcact
     5881 tcgcatcgtg aaagatcacg ccaacggtgt ggcgatggcc ggagcggatc cggctgacgc
     5941 catgacgcag gttaacgcga tagaacccgc gcgtcccctg cattggcctg acatgcacag
     6001 caaaagccac ggcatcgccc tgggcgagcg gcaccacttc gggccgcgac tgcatccgcg
     6061 gccgctgctc ggtcagcacg aattcaccgc cggtgaaatc ccggcccggt tccgacaaca
     6121 ggatcgcgac ctgcagcggg aaaacatgtt cgccatagag atcctgatgc aggcagttgt
     6181 agtcgccgac gccatattgc agcagcagcg gtgtcggccg ggtctggccg gcggcatgac
     6241 atcgcttcag gaacgcatca tggctcctgg gatagcggat atcgatgccc atggcctcgt
     6301 tccagctgtt agccacgtcg tgcagttgcg catacaacgc gggacgcaat tccgcgatca
     6361 ggtcgggcag cggataggaa aaatatttgt attcgccgcg cccgaacccg tgccgtccca
     6421 tcaccacccg gctgcggaac cgactgtcgt ccggatagac ggccgctagc gcgcggcatt
     6481 cttcgaccga aagcaatccc ttcaggacag cgcagccctg cgcatcgaga tcggtgcctg
     6541 cctgggtcca gtcgatggca tcgacgcgcg cggcaatatc ggaagcaggc gcgggatgga
     6601 tgatattttt tgcggttgat ttcatggcga ccaatgtcgc aaaacagccc agtctccgcc
     6661 acccgatttc cgaaaaggaa tcgaacccag ccgtgcgccg attcggcccg acggcagcgc
     6721 cgtcatgccg ggtttcggcc cggccatccg cgcctcaagc gatccgccga tccgaggacg
     6781 tggatgcccg gcgcgcgccg ggcatgacga tttcaatata agtcagccga gcacgggaag
     6841 ttcaatgaca tcgtagccat ggcgaatgct gcgcttgaga acggggtctt cgagctcaaa
     6901 ctcaaagcgg ctggccggcc ggatgccgcc cttggccgca aaggtaccgc cgaacatggc
     6961 gcagccgtca ggaaatccgg cgccgccaaa gccgcgctcg atcagttccg cgaccgggag
     7021 catgctgtcg agtgtgcctt cctggtacag cacccgcgcg ccgtcgatcc aggcatacga
     7081 acgcaggatc atttgatccc agtgaccgac gacctcctcg agttcccaca gcacgggcgc
     7141 gatcggcttg tcgcacatct gcttggagac cgtgacgcta taggcctcca ccttgcggtc
     7201 ggtgtggtcg gaggcggcgc cgacaaagat gcggccctgc cagccgatca gcacaaactc
     7261 aacctcgccg ctcgattccg cgccgctgac ttcgatgcgg tcttcggtgg tcagccgtcg
     7321 cgcggcgacg cgataataga tcggcgtcga cgcaggccgc gcaatgccga tcgcttcgag
     7381 ttcggcgatg tgcttgtcgc gcgccaccgg atcgcggccg gtccagccgg cgatgacggc
     7441 ctgatcgatc gccagcgtca gcggcgtggt cgtgccctgg acgtcgatgg tgaaggtaag
     7501 atcaaacacg gatggcatcc tctattccgg ctgcgagttc gaagatgcgg cgatccgatc
     7561 cgcccgcgcc cgcgagcatc agcccgaccg ggacctcgcc ctggcgatgg cagggcagcg
     7621 aaatcgcgca tccgtcgatc atgttgatca gcgtgcaatt gcgcagcgcc agcaggtttt
     7681 gcgcggtaaa cgccttgtcg tcggccagat ccgcgatgcg cggcggggtg ttggcggtgg
     7741 tcggcaagat cagcgcatca tggggtgcaa tacgtttttc cgtgcgtgcg atcagcgacc
     7801 gccgcgcgcc gagcaaatcg atataatcgg cagcgctgat ggcctcgccg cgaaggatgc
     7861 gtgtgcgtac gcggggatcg tagacatcgc catggctgac gatcagatag cgatgccagg
     7921 catagctttc cgccgcggca aaaccgccct tggcgttcat catcccgaca tcgaggaatt
     7981 ccggtacctc gatccgctcg atcagcgctc cctgccgcga cagcgtctcc agcgcgcgtt
     8041 cgaatgtccg cgccacggcg tcttcgagtt ggtcgagaac gaccgtggtc ggaaccgcca
     8101 gacgcatccc cttgatcggg cgcggctgca gcggcttcac cggctcgccg gcgagaacgg
     8161 cgtcgagcac cgcgcagcag cccaccgtgc gcgccagcgg cccaaagctg tcgagcgtcg
     8221 acgacagcgg cacaccgccc tcgagcggca cccggcgctg cgtcggcttg aacccgacaa
     8281 tgccgttgaa ggccgccgga atccggcacg agccgccggt gtcggtgccg agcgcgccat
     8341 gcgccatgcc atcgacgacg gagaccgcgg cccccgatga cgatccgccg ggcacgtggc
     8401 ccacatggcg ccgccatgcg cccttcgggg tgtcgaaatg cggattgatg ccgatgccgg
     8461 aaaaagcgaa ttcggtcatg ttggtgcggc cgatcacgac aaagccggcg cggcgcagcc
     8521 gcgccaccac cgatgcatcg gcttccgccg gggctgcatc ctccagcgcg cgcgaaccgg
     8581 cgcgggtcac ctgccccttg atgtcgaaca gatccttgat cgagacggga atgccggcgt
     8641 agcgcgacgg cgccgccttc acctcgcgca gccggtccat cgcttcggcg gcctcgatcg
     8701 cggcttccgc atcgacatga atgaaggtcc gcatcccctc gcctgaggtg tcggcgatcc
     8761 tggcgagaca atcgtcgacc agtttgcggg cgctggtgcg gccaccttcg aggtcatcag
     8821 ccagcgtcgc cagcgtcgga gtgtttggca tcgtactttc gcctcatggc tgcagaaccg
     8881 actattcgtt gtccgaaacc gcgtcgatct ccagatcgag cagcgccgag ctgagggatt
     8941 tgccatgggc atcgagcgcc agcgagcgcg tgaccccgcc gccgagcgcg ccggccatga
     9001 caaaattcag cgcggcaatg ttgggcagct catagcggac aacctcgcct tcgacaacac
     9061 cggcgaagtg agccttcacc cgcgcgctgg tgacttgcgc gagcagcagc ggataatgct
     9121 tctcgtgata agcaatcacc gagatattcg atgtgttgcc cttatcgccg gtgcgggaat
     9181 gggcgatctc gcgcagcttc acgtctcacg ctcccacaaa acggattatc ggcttggcca
     9241 gttcgcgcgg cagcaggact gaagcaaccg cgacgacgtc acgcgccgat ttgaaagcgc
     9301 cgccgccggc ggcggggcca ttggtgtaga gcgtttcaac ctcgttgccg atccggatgg
     9361 cctcacgcag attctcggtc cggccgacaa cgcgaacgcg cacctcgtag ggctcgttcg
     9421 cccgcgcgga gatttccgtc ccgtgcagcg aatcgacgcc gatcagttcg aaccgcagtt
     9481 cgcttgatgc aacgtcggtg agtttcagcc gctcccggac gatgtccagc gccagccgtc
     9541 cgcgcgccaa ggcgccggga ccggcatagg aaatctgccc ctcgccaata aaactgtcga
     9601 catagccgac cgagactttc agcgtgtcgg tgcgcccagt cccgcgcccg ccgctgatcc
     9661 gcacgcggtc ggtcgcgatc tcctcgacct ttacttgcga aaaatccgcg accacgtccg
     9721 gctgaatata gcgcttggga tcgtgcacct cgtagagcaa ttgctccttg caggtctgcg
     9781 c
//
LOCUS       DATNOI010000666         4172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_87679,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000666 DATNOI010000000
VERSION     DATNOI010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87679"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VN968_26790"
     CDS             <1..693
                     /locus_tag="VN968_26790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL32872.1"
                     /translation="ALALADSGFDIALVDLLEKEMAATARDIEARGKRTLTFQADVSD
                     FARAHAIVEQVITKWSQLDVLLNNAGAPNPKPILEISEAEFDRTIAVNLKSCFNFIHA
                     AAPIMLKQDTGGRIISTSSINALSGGVTSAVSKHAYAAAKAGILGLTRSLAKELGPKV
                     SVNAICPGVVATDLLRGLLGEREAQLTAGIALGRVGTPEDVASIVRYLAVEAHMFMTG
                     QHFVVDGFQWSC"
     gene            1033..2397
                     /locus_tag="VN968_26795"
     CDS             1033..2397
                     /locus_tag="VN968_26795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL32873.1"
                     /translation="MAERVLDVAALIETRPIGLAQRTVIALCAFVAMLDGLDLQSIGL
                     AAPAMGGELHIAPQAFGPVFSAALAGLALGAFLFGPVADRAGRKRVLVGATFCFGFFT
                     LATAYAGSLPELLACRFLAGVGLGGAMPSFISLASEYVPKARRAAVVSLLWAGFPLGG
                     VVGGLLGSRIIPAYGWQSIFVVGGVLPILSSMLLAIALPESVSFLVATGKSEYRIGRT
                     LRRIFPDVSTAGEIRFEFVKEIGPRATLPQLFRSGRAAVTLLLWASFFFAFMILVTNS
                     SWSPILLRRVGIAAEQSALALALFNFGSLFGSAAAGMLLNRFGTLRVLPPTLTLGALA
                     YATVGWSAPSLDAVMIAQGLFGLLLGCASSGLIALSAIYYPVAIRSTGVGWATGIGRL
                     GSVAGPLAVGQMVGAQWDVPTIFLAVGGSVLIGAAASALMGLLPRAGATDALAATPTA
                     SLHA"
     gene            2414..3403
                     /locus_tag="VN968_26800"
     CDS             2414..3403
                     /locus_tag="VN968_26800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32874.1"
                     /translation="MSTLQLSVAIGDYDRMRPLVDGLVQIDGVDPQFMLLEPEEIFFR
                     AFRHADFDICELSLSSYSVKIAAGTSPYVAVPVFPSRAFRHTSIYVRTDRIKSPADLK
                     GCRIGVPEYQLTANIWVRMILEDDYGIRPADVTWVRGGYEQPGRVEKISLNLPADVVV
                     EDAGPDDTISALLASGELDAVIGPRAPSCFQHGHPDVGYLFADPRQAAADWYRRTGLF
                     PIMHTLGIRRSLAEKHPWLPIAVFKAFERSKTIALSKLGDTSAAKVTLPFVEEQLRAA
                     RLLMGEDFWSYGLEPNRGVLTRFLQRHHAEGLSARLLTPEDLFHPASLELHKI"
     gene            3591..>4172
                     /locus_tag="VN968_26805"
     CDS             3591..>4172
                     /locus_tag="VN968_26805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32875.1"
                     /translation="MLVRSVGRDQRPWNKGLLIRQKRSLQSKHVWSISVRLEIARSWR
                     DLVIFNLAIDSKLRACDLVTLRLDDICSGAKVRHRATIVQKKTGRPVQFEITKQSRSS
                     VEAWLPMLRTTGGLESTSYGTRSMRRTKAAQIDRKIGNLRAVQFLPGHTKLESTVRYL
                     GIELNDALNIAGHRALIVDPPASCRWSLAHQRRS"
BASE COUNT          725 a         1267 c         1310 g          870 t
ORIGIN      
        1 gcgctggcgc ttgccgattc cggcttcgac atcgccctcg tcgatctcct ggagaaggag
       61 atggcagcaa ccgcccgcga tatcgaggcg cggggcaagc gcacgctgac ctttcaggcc
      121 gatgtgagcg attttgcccg cgcgcatgca atcgtcgagc aggtcatcac aaaatggagc
      181 cagctcgacg tgctcctgaa caatgcgggc gcgccgaacc cgaaaccgat cctcgagatc
      241 agcgaggccg agttcgaccg cacgatcgca gtcaatctca agagctgctt caacttcatt
      301 cacgcggccg cgccgatcat gctgaagcag gacacaggtg gacggatcat ctcaacctct
      361 tcgatcaatg cgctgtcggg cggcgtgacc agcgctgtca gcaagcatgc ctatgccgcg
      421 gccaaggccg gaattttggg cctcacccgc tcgctcgcaa aagagctcgg gccgaaagtg
      481 tcggtcaatg cgatctgccc aggcgttgtc gcgaccgacc tgttgcgcgg cctgctcggc
      541 gaacgggagg ctcagctgac cgccgggatt gcgctgggcc gggtcggtac gcctgaggac
      601 gtcgccagca tcgtgcgcta tctggccgtc gaggcgcata tgttcatgac cggccagcat
      661 ttcgtggtcg atggatttca atggagctgc tgagcagagc tgcgccccga ctgacgagcg
      721 catgatccgc aaaagtgtgc agcggttttc cgcgctgcgg gtgccactct tgacaagcat
      781 ggcgacaggc ttgcggtcaa aataggtgaa atttgcaatg tcgttgcgga ttccgatagc
      841 atttgcgccg atcgccgcga cgaccgagtc gagcacaccc tggcgccgtc cggtgacgta
      901 gacgaaagca cgttcggcgg cgaataattt tgcggttaca gcgaagcttc tcgaaaaggt
      961 catcgctaat ctggaaaagc agtgatctga accggaaacc acgccggtca gcggccggga
     1021 acggaggaaa acatggcgga acgggttctg gatgttgccg cattgatcga gacgcgcccg
     1081 atcggtcttg cgcagcgaac cgtgatcgcg ctctgcgcct tcgtggcgat gctggatggt
     1141 ctggatctgc agtcgatcgg ccttgccgcg cccgcgatgg gcggagagct tcacatcgcg
     1201 ccgcaagcct tcggcccggt gttcagcgct gctctggccg gccttgcctt gggcgccttt
     1261 ctgttcggac cggtcgccga tcgggccggc cgcaagcgcg ttcttgtcgg cgccaccttc
     1321 tgcttcggat tttttacgct ggcaacggcc tacgccggca gtctgcccga actgcttgct
     1381 tgccgctttc ttgccggcgt cggactgggc ggcgcgatgc cgagtttcat cagtcttgcg
     1441 tcggaatatg ttccgaaagc gcgccgggcc gccgtggtca gcctgctttg ggcaggcttt
     1501 ccgcttggcg gcgtggtggg cgggctgctc ggatcgcgca tcattccggc ctatgggtgg
     1561 caatccattt tcgtggtcgg cggcgttctg ccgatcctgt cgtcgatgct cctcgcgatc
     1621 gcgcttcctg aatcggtgag cttcctggtc gcgaccggca agtcggaata tcggatcggc
     1681 cgcacgctgc gtcgtatctt ccctgacgtg tccaccgcgg gcgaaattcg cttcgaattc
     1741 gtcaaggaaa tcgggccaag agcaacgctg cctcagctgt tcagatccgg gcgcgcggcg
     1801 gtcaccctgc tcctgtgggc aagctttttc ttcgcgttca tgatcctggt gacgaattcg
     1861 tcgtggagcc cgatcttgct gcgccgcgtg ggcatcgccg ccgagcaatc cgcgctggcg
     1921 ctggctctct tcaatttcgg ctcgttgttc ggttcagctg cagccggcat gctgctgaac
     1981 cgtttcggga ccttgcgcgt gctgccgccg accctcacgc tcggcgcctt ggcttatgcg
     2041 acggtcggat ggtccgctcc ctcgctcgac gccgtcatga tagcgcaggg cctgttcggc
     2101 ctgctgcttg gctgcgcgag ttcgggcctg atcgcactgt ccgccatcta ctatccggtc
     2161 gcgatccgct cgacgggtgt cggctgggcc acagggatag gccggcttgg atcggttgcg
     2221 gggccgctcg cggtcggcca gatggtcggt gcccaatggg acgtgccgac gatcttcctg
     2281 gccgtgggcg gatcggtgct gatcggcgcc gctgcgagcg cgctgatggg cctgcttccg
     2341 cgggccggcg ccacagatgc gcttgcggcc actccaactg cttccttgca cgcttgatgt
     2401 cgatgggacg ctcatgagca cgctgcaact ttcggtcgcg atcggcgact atgaccggat
     2461 gcgtcctttg gtggacggct tagtgcagat cgatggtgtc gatccacaat tcatgttgct
     2521 ggagccggag gagatattct ttcgcgcgtt caggcatgca gatttcgaca tctgtgaatt
     2581 gtcgctcagc agctacagcg tcaaaatcgc cgcgggaacc agcccctacg tcgcggtgcc
     2641 ggtttttcct tctcgcgctt tccgacacac ctcgatctac gtccgtactg accggatcaa
     2701 gtcccctgcc gacctcaagg gctgccgcat cggggtgccg gaatatcagc tcacggcgaa
     2761 tatttgggtc cgcatgatcc tggaggatga ttacggaatc cgtccggcgg acgtcacctg
     2821 ggtccgaggc ggctatgagc agccgggacg agtcgagaag atcagtctca acctgccggc
     2881 tgacgttgtt gtggaggatg cgggccctga cgacaccatt tcagcgctgc tggcaagcgg
     2941 agagctggac gcggtgatcg ggccccgggc accgtcctgt ttccagcacg gccatcccga
     3001 tgttggctat ctgtttgccg acccgcggca ggcggcggcc gactggtatc ggcggaccgg
     3061 cctttttccc atcatgcaca cgctaggtat ccgccgctct ctggccgaaa agcatccctg
     3121 gctgccgatc gcggtcttca aggcgttcga acgctccaag acgattgcgc tctcgaagct
     3181 gggcgacact tccgccgcca aggtgacgct gccctttgtg gaggagcagt tgcgggcggc
     3241 ccgcctcctg atgggcgagg atttctggtc gtatggattg gagccgaacc gcggcgtgct
     3301 aacccggttt ctccaacgcc accacgcgga gggattgtcg gcgcggctac taactccgga
     3361 agatctgttc catccagcct cgctggagct gcacaagatc taatgtctgt cacagaacaa
     3421 gcgatgatgc cgttcaggcg aacgaacacg ccgcgctcag cgagtgttcc ttacctggct
     3481 tccatgtggt cgatcgggaa ttctgtttgg gggcgccaga atgctgagtc gtcataaata
     3541 atacgctcgc gatagagcga cgcggagggc ggggtggcgg aggaacaccc atgcttgtac
     3601 gttctgtcgg cagagaccaa cgaccttgga acaagggatt gctcatccgc cagaaacggt
     3661 ccctccaatc gaagcacgtc tggtccatta gcgttcgcct agaaatcgca cgatcgtggc
     3721 gcgaccttgt catctttaac ctggccatcg acagtaagct tcgcgcatgc gacctggtca
     3781 cacttcgatt agacgacatt tgctcaggag caaaagtgcg gcatcgagca acgatcgtcc
     3841 aaaagaagac aggtcgacca gtccagttcg agatcacgaa gcagtcgagg agttccgttg
     3901 aagcctggtt accaatgctt cggaccactg gcggcttgga gtcgacctct tatggcacgc
     3961 gctcgatgcg gcgcacgaag gccgcccaga tcgatcgtaa gattggcaac ctgcgcgctg
     4021 tgcaattcct ccccgggcac acgaagctgg agagtaccgt ccgatatctc gggattgagc
     4081 tgaatgacgc tctaaatatc gctgggcatc gagctctaat cgtcgaccca ccggcatcat
     4141 gccggtggtc gcttgcgcac caaaggcggt cg
//
LOCUS       DATNOI010000667         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_28904,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000667 DATNOI010000000
VERSION     DATNOI010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28904"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VN968_26810"
     CDS             <1..487
                     /locus_tag="VN968_26810"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HXL32876.1"
                     /translation="IQPSRAQPTTPAPPSTQPSTDVARPASDTAARISRIVREVDDAA
                     AETPLGDWQTEGKGSVRIESCGRALCGHVLNPSSNDNGEMVLINMKPKTAALWSGKIY
                     SRDSENTYYATIAMSGPDSLWVKACALGKFYCSGNLWSRIGGKPEKLVISRQTSPQPR
                     S"
     gene            complement(579..788)
                     /locus_tag="VN968_26815"
     CDS             complement(579..788)
                     /locus_tag="VN968_26815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32877.1"
                     /translation="MLPAARGGAEAASDSSADFFRREPFVGFGVTAGSDVNQGGFLVT
                     LVGRLSVVTAYVAIAFVGAIVLGVF"
     gene            853..2079
                     /locus_tag="VN968_26820"
     CDS             853..2079
                     /locus_tag="VN968_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HXL32878.1"
                     /translation="MARGVRLYLVGSFVLVSLAGCGRGFFQSAEREPWRTEAEIACLK
                     SGAVKENADRVRINPISGPGICGAEYPLKVAALGESSGSFGFADDSLRPPASIGTQPR
                     WPISPPPQPPLPSSYPDAVVRQPYGAPSRGPISLTAPGVAAQQDEINLAPEGTPGLNQ
                     PGPQKRYLGPYMGTQPYPPRERYSAPRPAPDLPQADRMPRLGPAQGNSVAAFGPVAVK
                     PAATLACPIVSALDRWLADSVQPAGQRWFGARVVEIRQISAYSCRGMNGNSWAHISEH
                     AFGNALDVAGFTLADGRQISVKDGWRGLPEEQGFLRDVEAAACQQFSTVLAPGSNAYH
                     YNHIHVDLMRRASQRVICQPAAVSGEEVAARASQRNPYASREPFATGSLVGRKAVPQP
                     HRPNNRVNEEDEFEDD"
BASE COUNT          385 a          689 c          665 g          340 t
ORIGIN      
        1 tattcagccg tccagggcac aaccgaccac accggcccca ccttcaaccc agccgtcaac
       61 cgacgtggcg cggcccgctt cggacaccgc tgcccggatt tccaggatcg tgcgcgaggt
      121 ggacgacgca gcggcggaaa cgccgctcgg cgactggcag accgaaggca aaggatcggt
      181 tcgcatcgaa agctgcggcc gcgcgctgtg cggacatgtc ctcaatccat cgtcaaatga
      241 caatggcgag atggtgctga tcaacatgaa gccgaagacg gcggcgctgt ggtccggcaa
      301 gatctatagc cgtgacagcg agaacaccta ctacgcgacg atcgccatga gcgggccgga
      361 ttccctttgg gtcaaagcct gcgcgttggg caagttttac tgttccggca atctctggag
      421 caggatcggc ggcaagcccg agaagctggt gatctcgcgg cagacatcgc cgcagccgcg
      481 gtcgtagcaa cttctacgtg gcaagcgcgc atgaaaacgc ccagctgctg gaatcttcca
      541 accgccgggc gccgtaaatt caggttatgc gtgaggtcct agaacacgcc gagcacgatc
      601 gcgccaacaa aggcgattgc gacatacgcg gtaacaacgc tcagtctgcc gaccaaagtc
      661 acgagaaaac ctccctggtt cacgtcgctg cccgcggtaa cgccgaagcc aacaaacggt
      721 tcccgccgga aaaagtcagc gctgctgtcg ctcgcggcct ctgcaccgcc gcgagccgca
      781 ggaagcatgg ttaaagagtc gtgaaatcaa ggcgttgaag agtccttaaa taaattcgtc
      841 gaacgtgcgc ggatggcgcg cggagttcgt ttgtatctcg tcggctcctt cgtccttgtc
      901 tcgttggcgg gctgcggccg tggatttttt cagtccgcgg agcgcgaacc gtggcggacc
      961 gaggccgaaa tcgcctgcct gaagtccggc gccgtcaagg agaacgccga tcgggtccgg
     1021 atcaacccga tttccggccc cggcatctgc ggcgcggaat atccgctcaa ggtcgcggcg
     1081 cttggcgaaa gcagcggcag cttcggtttt gccgacgaca gccttcgccc gcccgcaagc
     1141 atcggcaccc agccgcgctg gccgatctcg ccgccgcccc agcctccctt gccgtcatct
     1201 tatcccgatg cggtggtgcg gcagccgtac ggcgcgccgt ccaggggccc gatttccctg
     1261 accgcgccag gggtcgccgc gcaacaggat gaaatcaatc tggcgcccga gggcacgcca
     1321 gggttgaacc agccaggccc gcagaagcgg tatcttgggc cgtatatggg gacgcagccc
     1381 tatccgccgc gcgagcgcta ttcggcgccg cgccccgccc ccgacctgcc gcaggcggat
     1441 cgcatgccgc ggctcgggcc ggcgcagggc aattctgtcg cagccttcgg gccggttgcg
     1501 gtcaagccgg cggcgacgct ggcctgtccg atcgtctcgg cgctcgatcg ctggctcgcc
     1561 gattccgtgc agcccgcagg acagcgctgg ttcggcgcgc gcgtggtcga gatcaggcag
     1621 atctcggcct attcctgccg cggcatgaac ggcaattcct gggcgcatat ctccgaacac
     1681 gcttttggaa atgcgctgga tgtcgcaggc ttcacgctcg ccgacggccg gcagatttcg
     1741 gtgaaggacg gctggcgagg cctgccggaa gagcagggct ttctgcgcga cgtggaggcc
     1801 gcggcctgcc agcagttctc aaccgtgctg gcgccgggct ccaatgccta tcactacaat
     1861 cacatccacg tcgacctgat gcgccgcgcc agccagcgcg tgatctgcca gccggccgcg
     1921 gtctcgggcg aggaggttgc cgcgcgggcg tcgcagcgca acccctacgc ttcgcgcgaa
     1981 cctttcgcga ccggatcgct cgtgggccgc aaggcggtgc cgcaaccgca cagacccaac
     2041 aacagggtca acgaggagga cgaattcgag gacgattag
//
LOCUS       DATNOI010000668         2734 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1633785,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000668 DATNOI010000000
VERSION     DATNOI010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633785"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VN968_26825"
     CDS             complement(<1..768)
                     /locus_tag="VN968_26825"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXL32879.1"
                     /translation="MISEAENELLTRVGRKTPMGKFMRQFWIPACLSSELEADGPQIR
                     LMLLGEKLIAFRDTAGRVGIMDHLCPHRLASLFLGRNEDHGIRCAYHGWKFDVNGNCL
                     DQPNLPDKNKYPAGVKAKAYKTTERGGIVFVYMGERAEAPPFPEIEAAVTTNPDDRNI
                     ALTHRECNWLQALEGDIDTSHLGFLHAGCVDPSKMDPNDAATYTVLNKAPQIHVSEMP
                     FGTMYSASRAAMEGHEHHRFACFIFPFWVCYPSDRLER"
     gene            complement(765..1838)
                     /locus_tag="VN968_26830"
     CDS             complement(765..1838)
                     /locus_tag="VN968_26830"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXL32880.1"
                     /translation="MTIEPWRATCIQTKSKLAVKAADRAGAWAIINDNIAHVIGLIEA
                     ATRGADPPDLVVLPEFSLQGPPHGLPAAEWIEKACCSVPGAITEPLQTLARRHRLYIG
                     GHQFESDPQWPGRYFNTSFLIDPTGAVILRYRRVNTAAFPSPHDFMDAYLARHSMAEI
                     FPVVDTGLGRLAMIPCGEINVPEVARVLMMQGAEVILHPTNSPRRPTQEAAKIVRAAE
                     NMIYIVSANVAEGIGFSWDGSVQGGRSHIIDYLGRTLAYEDGAGETTSVSALIDVEAL
                     REARKQDTGTANPLLRARWEMYRPFFATASFYPPNSFLHTPMSDAASAGPALAASLRN
                     MASTGVIVDEPGVPIKKRAGEAR"
     gene            2072..>2734
                     /locus_tag="VN968_26835"
     CDS             2072..>2734
                     /locus_tag="VN968_26835"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXL32881.1"
                     /translation="MRLILALLGSSLIVLAQPAASQTYPAKPIHLLVPYAPGGPADIA
                     ARLVGAKLTEAWGQQVVVENRPGGNGFIAMTAAAKSAPDGYTLVMATIGEAAVSPVLF
                     KEVPYNIDRDFAPISLISDATIVLATHGEAPFQSVADVIAAAKAQPGRISVGSPGNGT
                     VNQIVLEWMALNTGTKFQHIPYKGSAPAANAIAANEIPLGMLASSSVAPHLKTGRVRV
                     LAV"
BASE COUNT          476 a          858 c          876 g          524 t
ORIGIN      
        1 gcgttccagc cggtcgctcg gatagcagac ccagaacgga aagatgaaac aggcgaagcg
       61 gtggtgctca tggccctcca tggccgcgcg tgaggccgaa tacatcgtcc cgaacggcat
      121 ttcgctgacg tgaatctggg gcgccttgtt cagcaccgta taggttgcag cgtcgttggg
      181 gtccatcttg gaggggtcga cgcatccggc atggagaaag cccaggtgcg aggtgtcgat
      241 gtcgccttcg agcgcttgca gccaattgca ctcgcggtgg gtcaaggcga tgtttctgtc
      301 gtcggggttg gtggtgacgg cggcttcgat ctcggggaag ggcggcgcct cggcgcgctc
      361 gcccatgtag acgaacacta tcccgccgcg ctcggtggtc ttataggcct tggccttcac
      421 gcccgcggga tacttgttct tgtcgggcaa gttgggctga tcgaggcaat tgccgttcac
      481 gtcgaacttc cagccgtgat aggcgcagcg gatgccgtga tcctcgttgc ggccgaggaa
      541 caacgacgcc aggcggtgcg ggcagagatg atccatgatg ccgacgcggc cggccgtgtc
      601 gcggaacgcg atcagcttct cgccgagcaa catcaaacgt atctgcgggc cgtcggcctc
      661 gagctcggag gaaagacagg ctggaatcca aaactgccgc atgaacttgc ccattggcgt
      721 cttgcggccg acgcgggtca ggagctcgtt ttcggcttcg ctgatcatct ggcttctcct
      781 gctctttttt tgatgggcac ccccggctcg tcgacgatga cgccggtgct tgccatgttg
      841 cgcaaggatg cggccagtgc cgggccggcg gaggctgcgt ccgacatcgg cgtatggagg
      901 aagctgttgg gcggatagaa cgacgccgtt gcgaagaatg gccggtacat ttcccagcgc
      961 gcgcgcagca ggggattggc cgtccccgta tcttgtttgc gcgcttcgcg cagcgcttcg
     1021 acgtcgatca gcgccgagac cgacgtggtt tctccggcgc cgtcctcgta ggccaatgtc
     1081 cggccgagat agtcgatgat gtgggagcgc ccgccctgca ccgagccatc ccacgaaaac
     1141 ccgatgcctt cggcgacatt cgcgctgacg atgtagatca tgttctcggc cgcgcgcacg
     1201 atcttggccg cttcctgggt cggccgtctc ggtgaattcg tcggatggag gatgacctcg
     1261 gccccttgca tcatcagcac gcgcgcgacc tccggcacat tgatctcccc gcagggaatc
     1321 atggcgagcc gcccaagccc ggtatccacc acgggaaaaa tctcggccat gctgtggcgc
     1381 gccaaatacg catccataaa atcgtgcggg gaggggaagg cagcggtatt gacccgccgg
     1441 tagcgcagga tgactgcacc ggtcggatcg atcagaaaac tggtgttgaa ataacggccc
     1501 ggccactgcg ggtcgctctc gaattgatgg ccaccgatgt agagtcgatg tcggcgggcg
     1561 agcgtctgca acggctcggt gatcgcgccg ggaactgagc agcaggcttt ttcgatccat
     1621 tccgccgccg gcaggccgtg cgggggtcct tgcagcgaga actccggaag aaccacgaga
     1681 tcggggggat cggcaccgcg ggtcgcggcc tcgatcagcc cgatcacgtg cgcgatattg
     1741 tcattgatga tcgcccacgc gccggcacga tcggcggctt tgacagcgag cttgcttttg
     1801 gtctggatgc aggtcgctcg ccacggctcg atggtcattg ctcggcgcct gtatgagatg
     1861 cggcaatcaa tcccatgatg atcgaatacg gattttctta caatagtacg gcaaacagct
     1921 acggctcgct tacggaccgt cgtgcccgct ttttcgtcag cctctgccga gcaacgcgcc
     1981 gccgcttcga ccgcgtctta tgctatggag gcattcaatc cgccgacgcg ctaccgcgtc
     2041 gctcactccg caaatggctc aggaggatca ggtgcgtctc atcctagcac tgcttggttc
     2101 gagtctcatt gtcttggcgc agccggccgc atcgcagacc tatccggcaa agcccatcca
     2161 cctgcttgtt ccttacgcac ccggcggccc cgccgatatc gccgcccggc tcgtcggtgc
     2221 caagctgacc gaagcctggg gccagcaggt ggtggtggag aaccggccgg gcggcaacgg
     2281 tttcatcgcc atgacggcgg cggcgaaaag cgcgcccgac ggttatacgc tggtgatggc
     2341 gacgatcggc gaagccgccg tcagtccggt gctgttcaag gaggtgccct acaatatcga
     2401 ccgcgatttc gcgccgatct ccttgatcag cgatgccacc atcgtgctcg ccacgcacgg
     2461 tgaggcgccg ttccagtcgg tcgccgacgt gatcgccgcg gccaaagcgc agcccggccg
     2521 catctcggtc ggctctcccg gcaacggcac cgtcaatcag atcgtgctcg agtggatggc
     2581 gctcaacacc ggtacgaagt tccagcacat tccctacaag ggcagcgcac cggccgccaa
     2641 cgcgattgcc gccaacgaga ttccgctcgg catgctggca agctcctcgg tcgccccgca
     2701 cctcaagacc ggccgcgtcc gcgttctggc ggtc
//
LOCUS       DATNOI010000669         3472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1865917,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000669 DATNOI010000000
VERSION     DATNOI010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865917"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VN968_26840"
     CDS             <1..235
                     /locus_tag="VN968_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXL32882.1"
                     /translation="NDWQVGQTGKVVAPELYIAIGISGAIQHLAGMKDSKVIVAINKD
                     EDAPIFQVADYGLVADLYQAVPELTEALGKLGK"
     gene            330..1208
                     /locus_tag="VN968_26845"
     CDS             330..1208
                     /locus_tag="VN968_26845"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HXL32883.1"
                     /translation="MAMIKKVGVIGSGQMGNGIAHVAALAGFDVVLNDVSLDRLKSAM
                     ATINGNLSRQVTKKIISEDARKQALDRIVSAETMEGLGDCDIVIETAVEKEEVKRKIF
                     HEVCAVLKPEAIVASNTSSISITRLAASTDRPERFIGIHFMNPVPLMELVELIRGIAT
                     DDQTFEASKEFVTRLGKHIAVSEDFPAFIVNRILLPMINEAIYTLYEGVGNVEAIDAA
                     MKLGAHHPMGPLELADFIGLDTCLSIMQVLHEGLADSKYRPCPLLVKYVEAGWLGRKT
                     QRGFYDYRGSKPVPTR"
     gene            1461..1655
                     /locus_tag="VN968_26850"
     CDS             1461..1655
                     /locus_tag="VN968_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32884.1"
                     /translation="MDMMSMVSSLLAMKAGGTQSQIAATILKSNADSEKSTVLTLLGA
                     GGSSLANVGAGVGGNLDISA"
     gene            complement(1773..2441)
                     /locus_tag="VN968_26855"
     CDS             complement(1773..2441)
                     /locus_tag="VN968_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HXL32885.1"
                     /translation="MPERPTPHPAATRRIPFAIGAVLIGAVIGFAAVSGIGGLKRNAA
                     GDSACRAAVELAGRIAPLAHGEVAALTMATTPLLLPDLAFEDADGKPKKLSDWRGRTV
                     LVNLWATWCVPCRREMPALESLQTRLGGPDFEVVAINIDTRDPEKPKNFLKDANLTRL
                     NYFSDQKAKVFQDLKAIGRALGMPTSVLVDGRGCEIATIAGPAEWASDDAVKLITAAM
                     TPPG"
     gene            2558..>3472
                     /gene="argH"
                     /locus_tag="VN968_26860"
     CDS             2558..>3472
                     /gene="argH"
                     /locus_tag="VN968_26860"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HXL32886.1"
                     /translation="MSNKMWGGRFTERPDAIMEEINVSIDVDRHMYAQDIAASKAHAA
                     MLAAQGIITATDAKNIGKGLDTILSEIGNGSFEFKRALEDIHMNVEGRLSELIGPAAG
                     RLHTARSRNDQVATDFRLYVRDTIDEVDAALAAFQRALVERATEHAGTVMPGFTHLQT
                     AQPVTFGHHLLAYVEMASRDRGRFADARKRLNESPLGAAALAGTSFPIDRTATAKALG
                     FDRPMANSLDAVSDRDFVLETLSAAAICAVHLSRFAEEIVIWTSPLVGLARLSDKFTT
                     GSSIMPQKRNPDAAELVRAKTGRVIGALT"
BASE COUNT          649 a         1057 c         1102 g          664 t
ORIGIN      
        1 gaacgactgg caggtcggcc agaccggcaa ggtggtggcg ccggaacttt atatcgccat
       61 cggcatttca ggcgccattc agcatcttgc cggtatgaag gattccaagg tgatcgtcgc
      121 gatcaacaag gacgaggatg caccgatttt ccaggtcgcc gattacggtc tggtcgcgga
      181 tctctaccag gcggttcccg aattaaccga agcgctcggg aaactcggaa agtaacgata
      241 atttaaccaa ccgggtgagg ggacttcggc cggtgttata aaggacgggc aggcgcgcga
      301 tgcacgcgtc gctccggtga gatgacaaga tggctatgat caagaaggtc ggcgtgatcg
      361 gctcgggcca gatgggcaac ggcatcgcgc atgtggcggc gctggccggt ttcgacgtcg
      421 tgctcaatga tgtgtcgctc gatcgcctga agtcggcgat ggcgaccatc aacggcaatc
      481 tgtcacgcca ggtcaccaag aaaatcatca gcgaggacgc ccgcaagcag gcgcttgatc
      541 ggatcgtttc cgccgagacg atggagggcc tcggcgattg cgatatcgtg atcgagaccg
      601 cggtcgagaa ggaagaggtc aagcgcaaga ttttccacga ggtctgcgcg gtgctgaagc
      661 cggaggccat cgtcgcctcc aacacctcgt cgatctcgat cacgcggctg gccgcgtcca
      721 ccgaccggcc cgagcgtttc atcggcattc acttcatgaa tccggtgccg ttgatggaac
      781 tggtcgaact gatccgcggc atcgccaccg atgaccagac cttcgaggcg tcgaaggaat
      841 tcgtgacgcg gctcggcaag cacatcgcgg tctcggaaga ttttccggcc ttcatcgtca
      901 accgcatcct gctgccgatg atcaacgagg cgatttatac gctgtatgag ggtgtcggaa
      961 acgtcgaggc gatcgatgcc gcgatgaagc tcggcgccca ccatccgatg gggccgctgg
     1021 aactggccga tttcatcggt ctcgatacct gtctgtcgat catgcaggtc ctgcatgagg
     1081 ggctggcgga ctcgaagtat cggccgtgcc cgctgctggt gaagtacgtc gaggccggct
     1141 ggctcggccg caagacccag cgcggcttct acgactatcg cggcagcaag ccggtcccga
     1201 cgcggtgaca ccaatcattg ccgagcatcg tcgtctgcct tgcgcagacg acgtaaactc
     1261 gtttgcgctc ccggcagtcc gtctcgcttc gaaaaatgca acttgtattg atggatgcgc
     1321 gggtcatttg gcgcgaggac gcgcttcgcg cttcatcccg cgcatgacga cctgaactcg
     1381 ttgcggtatt gtcctacaat ctccagcatt cattaactac cgccgggata gtgtgccggc
     1441 cggcgttggg agtgttgcgt atggatatga tgagtatggt cagtagcctt ctcgccatga
     1501 aggcgggtgg cacgcagagc cagatcgcag ccacgatctt gaaatcgaat gcggattccg
     1561 agaaatccac ggttctgacc ctgctgggcg cgggcggctc gtcactggcc aatgtcggcg
     1621 cgggtgtcgg cggcaatctc gatatttcag cctgaatctc ttctttcgtc attccgggat
     1681 gcgcctcttg gcacaggcgc ggaatccata ctcccagcag gggttacggt ttccgggctc
     1741 gctcgtttca ctcgcgcccc ggaatgacaa ggctagccgg gaggcgtcat cgccgctgtg
     1801 atcagcttga cggcgtcgtc gctggcccat tccgcgggtc cggcgatggt ggcgatctcg
     1861 caacccctgc catccaccag caccgaggtc ggcattccca gtgcccggcc gattgcctta
     1921 agatcctgaa aaaccttggc tttttggtcg ctgaaataat tgagccgggt caaattggcg
     1981 tcttttagga aattcttcgg tttttcggga tcgcgggtgt cgatattgat ggcgaccacc
     2041 tcgaagtcgg gtccgccgag cctggtttgc aggctctcca gcgccggcat ctccctgcgg
     2101 cagggcacac accaggtggc ccacaggttc accagcacgg tccgtccacg ccagtcggag
     2161 agctttttcg gcttgccgtc ggcgtcctcg aaggcgaggt ccggcagcag caagggggtt
     2221 gtcgccatcg tcagcgcggc gacctcgcca tgcgccagcg gcgcgatcct gccggcaagc
     2281 tcgaccgccg cgcggcaggc cgaatcgccc gctgcattgc gtttcagccc gccgatcccg
     2341 gatacggcgg caaatccgat gaccgccccg atcagcaccg ccccgatcgc gaagggtatc
     2401 cgccgcgtgg cagccggatg aggtgtgggt cgttcaggca tatcgtttgt catcctgtat
     2461 cagatacggc tatgcagagt tttggcggcg ccggtaacgg caccgagcgt tcgcttcgcg
     2521 ggcgtgacca tgagtggcag gtcgtgagca acaagtcatg agcaacaaga tgtggggcgg
     2581 ccggttcacc gagcgccccg atgcgatcat ggaggaaatc aacgtctcca tcgacgtcga
     2641 tcgtcacatg tacgcccagg acatcgccgc gtccaaggcc cacgccgcga tgcttgccgc
     2701 gcaaggcatc attaccgcaa ccgatgcgaa aaatatcggc aaaggtctag acacgatttt
     2761 gtcagaaatc ggcaatggat cgttcgaatt caagcgcgcg ctcgaggaca ttcacatgaa
     2821 tgtcgagggt aggctaagcg aactgatcgg gcctgcggcg gggcggctgc acaccgcgcg
     2881 gtcgcgcaac gatcaggtcg caaccgattt ccggctctac gtccgcgaca cgatcgacga
     2941 ggtggacgcg gcgctggccg cgttccagcg cgcgctggtg gaacgggcga ccgaacatgc
     3001 cggcaccgtg atgccgggat ttacccacct gcagaccgcc cagccggtga cgttcgggca
     3061 tcaccttctg gcctatgtcg agatggcctc gcgcgaccgc ggccgcttcg ccgacgcgcg
     3121 caagcggctc aacgaatcgc cgcttggggc ggcggcactc gccggcacct cgtttccgat
     3181 cgaccgcacg gccactgcga aagcgctcgg tttcgaccgg ccgatggcca attcgctcga
     3241 cgcggtctcc gaccgcgatt tcgtgctgga gacgctgtcg gcggcggcga tctgcgcggt
     3301 gcatctgtcg cgattcgccg aggagatcgt gatctggacc tccccgctgg tggggctggc
     3361 gcggctgagc gacaaattca ccaccggctc ctccatcatg ccgcagaagc gcaacccgga
     3421 cgcggcggaa ctggtgcgcg ccaagaccgg gcgggtgatc ggcgcgctca cg
//
LOCUS       DATNOI010000670         3994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1971194,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000670 DATNOI010000000
VERSION     DATNOI010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1971194"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VN968_26865"
     CDS             complement(<1..511)
                     /locus_tag="VN968_26865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HXL32887.1"
                     /translation="MPANRAVGLSLGAAILLCSSSLNLAYAKPFMIVGNDEKVWWDDD
                     GKTVLSAPGKDTVLIVDLANPESPQIVATLPLKNSIVGPPVNLDIDPTGSVALVADSV
                     DVTKDGDTMKQVPDNKIYVIDFKANPPKVAATVIGGKQPSGLSFSPSGKMALVANRGD
                     NSVSVLSVNG"
     gene            complement(649..1467)
                     /locus_tag="VN968_26870"
     CDS             complement(649..1467)
                     /locus_tag="VN968_26870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HXL32888.1"
                     /translation="MLSKSLPNVQATAEVTGGSVDNLKLIASGQSEMAFTMADAALDA
                     LQGQDKFKSGKVPLQALLVVYPNRMHVVTVEGSGIETMADLKGKRVSTGSPGGATEVM
                     AFRVIEAAGLDKDKDMKRERLGVAESVNAIKDRKIDAFFWVGGIPTAAVTDLAATPGL
                     KMKLIDHSETVEKMNAKYGKLYTASKIRAGAYPGTEKDNAIAEVWNLIVTGDKMSSDD
                     AYAIVKTLVEKKADIVAVHKEAESFSLDNQVQERSPIPFHPGALKYFKEKGLGG"
     gene            complement(1398..2012)
                     /locus_tag="VN968_26875"
     CDS             complement(1398..2012)
                     /locus_tag="VN968_26875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32889.1"
                     /translation="MAADKSAPGNTSPNDDVIAVSDEALLKAESFVEAEEGVANRLMG
                     WAGRISTAIAVVMSLFHLYAAYAIVPTQELRYTHVAFTLVLSFLLFPLATRFRNRVRW
                     WDVVPGIFAVATIFYALWGGDDFTDRATLPDRWDVIVGVIFIVLLLEATRRTTGAIMP
                     VVSLLFIAYAMFGPYLPARSPMCCRSRFPTYRPPPKSPAVRSII"
     gene            2186..2278
                     /locus_tag="VN968_26880"
                     /pseudo
     CDS             2186..2278
                     /locus_tag="VN968_26880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
     gene            2429..3232
                     /locus_tag="VN968_26885"
     CDS             2429..3232
                     /locus_tag="VN968_26885"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXL32890.1"
                     /translation="MERLKGKTAIVVGAGSIGPGWGNGKATAVTFAREGAQVFCVDRN
                     QAAAQQTVDIIASEGGAATAFTADAASASDVEAVVAACLKAYGRIDVLDNNVGIAEMG
                     NVVDVSEASWDHVFNVNLKSAFLAMKHVIPVMARQGGGSIINISSIASIRHLGISYVT
                     YATTKAAMNQMTRTTAVEYAPKNIRVNAVLPGLMKTPMVEHSAGLAASYAAGDVEAMW
                     RARDAQVPMGHMGDAWDVANAALYLASDESRYVTGIELVVDGGITLKAG"
     gene            complement(3412..>3994)
                     /locus_tag="VN968_26890"
     CDS             complement(3412..>3994)
                     /locus_tag="VN968_26890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HXL32891.1"
                     /translation="YGTTMRFLIARGIVHPRRPTAVPTVEGGIVVSWCGMRGIVTLAA
                     AYALPEGFPYRDLMLLTAFAVVLGSLVIQGLTLRPLISALKLRDENPVASEVARARAV
                     AYRAALDEIDGDPSEEAEILRLEYRALLLRAEGDPDGGVSTGELPADPLRRRAIGAAR
                     RSLLRLRQSETIGDDAFHQLEEEFDWAELSAQG"
BASE COUNT          755 a         1238 c         1259 g          742 t
ORIGIN      
        1 taccgttgac cgagagcacg ctgaccgaat tgtcgccgcg gttggcaacc agcgccatct
       61 tgccggacgg gctgaagctc agccccgacg gctgtttgcc gccgatgacg gtcgccgcca
      121 ctttgggtgg attggccttg aaatcgatga cgtaaatctt gttgtccggc acctgcttca
      181 tcgtgtcgcc gtccttggtg acatcgaccg aatccgccac cagcgcgacc gagccggtgg
      241 ggtcgatgtc gagattgacc ggcggtccga cgatcgaatt cttcagcggc agggtggcga
      301 cgatctgggg actttcagga ttggcgagat cgacgatcag aaccgtgtcc ttgccgggcg
      361 ccgacaggac cgtctttccg tcatcgtccc accagacttt ctcgtcgttg ccgacgatca
      421 tgaacggctt ggcataagcc agattaaggc tggagctgca gagcaggatt gccgctccca
      481 aggataatcc aacggcacga ttggcaggca ttgaggcgtt ctcccggata gaccggcctc
      541 tgatgggccg atttgcaatc ctagaatacg cggggaaccg cattgttgcc agtgcgaagg
      601 cggccgctat cgatgccgac ggcggccacg tcgcgcgaac cggaggcgct agccgccgag
      661 gcctttttcc ttgaagtatt tcagtgcacc gggatggaac gggatcggtg agcgctcctg
      721 gacctggttg tcgagcgaga agctttcggc ttccttatgc acagcgacga tgtcggcttt
      781 cttttccacc agcgtcttga cgatcgcata ggcgtcgtcg ctgctcatct tgtcgccggt
      841 aacaatcaga ttccagacct cggcaatggc gttgtccttc tcggtgcccg gataggcgcc
      901 ggccctgatt ttgctggcgg tatagagctt gccgtatttg gcgttcatct tctcgactgt
      961 ctcgctgtgg tcgatcagct tcatcttcag gccgggagtc gccgcaagat cggtcaccgc
     1021 agcggtggga atgccgccga cccagaagaa agcgtcgatc ttgcgatcct tgatggcgtt
     1081 gacggattcg gcgacgccga gccgctcgcg cttcatgtcc ttgtccttgt cgaggcccgc
     1141 cgcctcaatg acgcgaaacg ccatcacttc ggtcgcgccg cccggcgagc cggtggagac
     1201 acgcttgccc ttgagatccg ccatggtctc gatgccggag ccctccacgg tcacgacatg
     1261 catgcggttc ggatagacga cgagcagcgc ttgcagcggc accttgccgc tcttgaactt
     1321 gtcctggccc tgcagcgcat cgagcgcggc atcggccatg gtgaacgcca tctcgctctg
     1381 acccgacgcg atcagtttca gattatcgac cgaaccgccg gtgacttcgg cggtggcctg
     1441 tacgttggga agcgacttcg acagcacatt ggcgaccgcg ccggcagata cggaccgaac
     1501 atcgcatagg cgatgaacag cagcgacacc acgggcatga tcgcgccggt ggtgcgtcgt
     1561 gtcgcttcca gcagcaacac gatgaatatg acgccgacga tgacgtccca gcgatcgggc
     1621 agtgtggcgc ggtcagtaaa atcatcgccg ccccacaacg catagaagat ggtcgcaacc
     1681 gcgaaaatgc cgggcacgac gtcccaccag cgcacgcgat tacggaaacg cgtcgccagc
     1741 ggaaacagta ggaaactcag caccagcgtg aatgcgacgt gggtgtagcg aagttcctgg
     1801 gtcggcacga tcgcgtaggc cgcgtagagg tggaacaggc tcatcaccac ggcgatggcg
     1861 gtcgagatcc gccccgccca ccccatcaac cggttggcca caccctcttc ggcttcgacg
     1921 aacgactctg ccttgagcag ggcttcgtcg gaaactgcga tgacgtcgtc attgggcgat
     1981 gtattgccgg gcgctgactt gtctgcggcc atgttccccc ggaacgcttt ctacatggcg
     2041 tagcctggct ggccgtgcct ttgattgcgc cgcattgacc ccattttggg gagccgtggc
     2101 aagggattat cggcagagac ttttggatta tggcaaccgc gcggatagtt aacgttgccc
     2161 gcggactaac gctggcgcag gcgcgcatga agggctgcga tattgccgaa cattgtcgga
     2221 gattttcgga catccgatcg gcgccaacgt tacgacgatg agtgcctgcg atcagtagcc
     2281 cggatgcagc caacgggtcg gcgcgaagcg ccgcccgatg acaggctccg cgcaatccgg
     2341 gaaaacgccg accaaaagtc ccggatttcg caagggctcc atccgggcta cggttatctc
     2401 agcgacgtca tcatcaaggg agaactcgat ggaacggctc aagggcaaga cagcgatcgt
     2461 ggtcggcgcc ggttcgatcg ggccgggctg gggcaatggc aaggcgaccg cagtgacctt
     2521 cgcgcgcgag ggcgcgcagg tgttttgcgt cgaccgcaac caggctgcgg cgcagcaaac
     2581 cgtcgatatc atcgcttccg aaggcggcgc ggcgacggcg ttcaccgccg atgctgcgag
     2641 tgccagcgat gtcgaggcgg tggtggctgc ctgcctcaag gcctatggcc gcatcgacgt
     2701 gctcgacaac aatgtcggca tcgccgagat gggcaatgtc gtcgacgtct cggaggcgag
     2761 ttgggatcac gtcttcaacg tcaatctcaa aagcgcgttc ctcgccatga agcacgtcat
     2821 cccggtgatg gcgcggcaag gcggcggctc gatcatcaat atctcgtcga tcgcctcgat
     2881 ccgccatctc ggcatctcct atgtcaccta tgccaccacc aaggccgcga tgaaccagat
     2941 gacgcggacg acagcggtcg aatacgcgcc gaaaaatatc cgggtcaacg cggtgcttcc
     3001 gggcctgatg aaaaccccga tggtcgaaca ctccgccggg ttggcggcga gttacgccgc
     3061 gggcgacgtc gaggcgatgt ggcgcgcgcg cgacgcgcag gtgccgatgg gccatatggg
     3121 cgacgcctgg gatgtcgcca acgcggcgct gtatctcgcg tccgacgagt caagatatgt
     3181 caccgggata gagctggtgg tcgatggcgg gatcaccttg aaggcgggat aggcttcttc
     3241 ctcatggtga ggaggcgctc ttgcgccgtc tcgaaccatg aggctccact cccggcctca
     3301 tccttcgaga cgcggccaag cggccgctcc tcaggatgag gggagagaga ttgcagcgcc
     3361 tcagtcacat tcgccgggac aacgcgatat ttgctacacc gctgcattac atcatccctg
     3421 cgcgctcaat tcggcccagt cgaattcttc ctcgagttgg tgaaaggcgt cgtcgccgat
     3481 ggtttcggac tggcgcagcc tgagcaggga tcgccgcgcg gcgccgatcg cgcgccgccg
     3541 cagcggatct gccggaagtt cgccggtcga aacgccgcca tcgggatcgc cttccgcgcg
     3601 cagcagcagc gcgcggtatt cgagccggag gatctcggct tcctcggatg gatcgccgtc
     3661 gatttcatcc agcgccgcgc gataggccac cgcgcgggcg cgcgcgacct ccgatgccac
     3721 cgggttctca tccctgagct tcagggccga gatcagcggc cgcaaggtca ggccctgaat
     3781 caccaacgag cccagcacca cggcaaacgc cgtcaacagc atgagatcgc gatatggaaa
     3841 accttccggc agcgcgtacg ccgccgccag cgtgacgatg ccgcgcatcc cgcaccagga
     3901 caccacaatg ccgccttcga cggtgggcac ggccgtcggg cgtctgggat gaacgatacc
     3961 tcgcgcgatc aagaaccgca tggtggtgcc gtaa
//
LOCUS       DATNOI010000671         2751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1033056,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000671 DATNOI010000000
VERSION     DATNOI010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033056"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..845
                     /gene="phnE"
                     /locus_tag="VN968_26895"
     CDS             39..845
                     /gene="phnE"
                     /locus_tag="VN968_26895"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HXL32892.1"
                     /translation="MSVVAIVPRRWKRPPLIKRAWLRWTIGVGAVLYLMLALGTTEVN
                     WTRVWEGLPRGARFFAAFFPPDFASRWSEIAEGILESLWMTVVSTVIGIAISIPVGIG
                     AAKNIAPAPVYFFCRGILAVSRSFQEIILAIFFVKLFGFGPFAGLVTLSFATIGFYGK
                     LLAEDIEDMDPSQAEAVRATGAGWLQWLNYGVQPQVMPRMIGLGLYRFDINFRESAVV
                     GIVGGGGIGATLNTAFDRYEFDSAAAILLIIIGIVMLVEYSSSYLRRWVQ"
     gene            845..1660
                     /gene="phnE"
                     /locus_tag="VN968_26900"
     CDS             845..1660
                     /gene="phnE"
                     /locus_tag="VN968_26900"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HXL32893.1"
                     /translation="MAIELRHGTKVWHRRERGAQFATWAAWLAGAAIFIYCWELISDK
                     TIWAFVADAPTQAADLAVRLVPPDWGFIHALWRPLWDTINIATLGTAMGIIMAVPVAY
                     CAARNTTPSVAFIRPAALFIIVSSRSINSLIWALMLVTIIGPGVFAGILAIGLRSIGF
                     CAKLLYEAIEEIDESQVEAVRATGASGAQVTAYGIVPQVLPAFAGISMFRWDINIRES
                     TVLGLVGAGGIGLPLNAAITMLAWTQVSLILLIIIATVIVSEWVSAKIRHAII"
     gene            1932..2525
                     /locus_tag="VN968_26905"
     CDS             1932..2525
                     /locus_tag="VN968_26905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXL32894.1"
                     /translation="MPSSALFLDRDGVINVDRGYIHRSDQFEFVPGIFELARFWTNEL
                     RRLIVVVTNQSGIGRGYFDERAYEDLSRWMCDRFAAEHTAIARVYQCPYHPLHGIGEY
                     RRDHPWRKPNPGMILQAVSDLDLDPAQSAILGDKMSDIEAGAAAGIGLRILVASHDAK
                     ESEGAPPHEVVANLSEALVLLRSRFALTEPDPPYEHP"
     gene            2613..>2751
                     /locus_tag="VN968_26910"
     CDS             2613..>2751
                     /locus_tag="VN968_26910"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXL32895.1"
                     /translation="MPKQTALIVGITGPDGAYLARFLLDKGYDVYGTSRNIGLARTDG
                     LR"
BASE COUNT          495 a          819 c          846 g          591 t
ORIGIN      
        1 agccgccagc cgcaccgcga ccgcatggcc gggttgacat gagcgtcgtc gcgatcgtgc
       61 ctcgccgttg gaagagaccg ccgctcatca agcgcgcgtg gctgcgctgg acgatcggcg
      121 tcggcgccgt cctctatctg atgctggcgt tgggcaccac cgaggtgaat tggacccgcg
      181 tgtgggaggg cctgccgcgc ggcgcacggt tcttcgccgc gttctttccg cccgacttcg
      241 cgagccgctg gagcgaaatc gccgagggta tccttgaaag cctctggatg acggtggtct
      301 ccacggtgat cggcatcgcg atctcgatcc cggtgggcat cggcgcggca aaaaacatcg
      361 cgccggcgcc ggtctacttt ttctgccgcg ggatcctggc ggtgtcgcga agcttccagg
      421 agatcatcct ggcgatcttc ttcgttaagc tgtttggctt cggcccgttc gccggcctcg
      481 tcacattgtc gttcgccacc atcggcttct acggcaagct ccttgccgaa gacatcgagg
      541 acatggaccc atcccaggca gaagccgtgc gggccaccgg cgcgggctgg ctgcaatggc
      601 tcaattacgg ggtccaaccg caggtgatgc cgcgcatgat cgggctcggc ctctatcgtt
      661 tcgacatcaa cttccgcgaa tccgcggtgg tcggcatcgt cggcggcggc ggcatcggcg
      721 cgacgctgaa cacggcgttc gaccgctacg aattcgattc cgcagcagcg atccttctga
      781 tcatcatcgg catcgtgatg ttagtggaat attcgtccag ctaccttcgc cgctgggtgc
      841 agtgatggcg atcgagttgc ggcacggaac gaaagtgtgg catcgccgag agcgcggcgc
      901 gcagttcgcg acgtgggcgg cgtggctcgc gggggcagcg atcttcatct attgctggga
      961 attgatctcc gacaagacga tctgggcctt cgtcgccgac gcgccgacgc aggcggccga
     1021 tctcgccgtg cgcctggtac cgccggactg gggtttcatc cacgcattgt ggcggccgct
     1081 gtgggacacc atcaacatcg cgacgctggg caccgcgatg ggaatcatca tggcggtgcc
     1141 ggtcgcctat tgtgcggcgc gcaacaccac gccgagtgta gcattcatcc ggccggcggc
     1201 gctgttcatc atcgtgtcgt cgcgctcgat caactcgctg atctgggcat tgatgctggt
     1261 cacgatcatc gggccgggcg tgttcgccgg catcctggcg atcgggctgc gctcgatcgg
     1321 cttctgcgct aagctgctct acgaagcgat cgaggagatc gacgaaagcc aagtcgaggc
     1381 ggtgcgcgcg actggcgcga gcggcgcgca ggtcacggcg tacggcatcg tgccgcaagt
     1441 cctgccggcg ttcgccggca tttcgatgtt ccgttgggac atcaacatcc gcgagtcgac
     1501 ggtgctgggc ctggtgggcg ccggcggcat cgggctgccg ctcaacgcgg cgatcaccat
     1561 gctcgcctgg acccaggtgt cgctcattct gctgatcatc atcgcaacgg tcatcgtcag
     1621 cgaatgggtt tcggccaaga tacgccacgc gatcatctga gaccgtgagg tccgccggca
     1681 gtttacaagc atgattgggt tggtttaatc gatttgggat tctctaaatc ggggcgacgc
     1741 aatgaaaaaa gtgcattttt gtgaccgcat cacatcgtgg aaaatcgtgt caatcgcacg
     1801 gtacgattgg gccatagttg gtcagtcaat ctgaggatca gtataaggag ctttgatacc
     1861 cggcgcgggt cgtgcgtgct accattggct tgagcaaagc tgcaatcgcg gtgtcgtaga
     1921 ttggcacagc catgcctagc agtgcacttt ttcttgaccg cgacggcgtg atcaatgttg
     1981 accgtggcta catccatcgg tcggaccaat ttgaattcgt gcctggaatt ttcgaattgg
     2041 ctcgcttctg gaccaacgag ctgcgccgac tgatcgttgt cgttaccaac cagtccggca
     2101 tcggtcgcgg ctatttcgac gaacgcgcct atgaagactt gtcgcgatgg atgtgtgatc
     2161 gctttgcagc ggaacacacg gcgatcgctc gagtctatca atgcccttat caccctctcc
     2221 atggcatcgg cgaataccgc cgcgatcatc cgtggcgcaa gcccaacccc ggtatgatct
     2281 tgcaggccgt gtccgatctt gacctcgatc ctgctcagtc cgcgattctg ggagacaaaa
     2341 tgagtgatat cgaggccggc gcggcggctg gtatcggtct gcgaattctg gtcgcatcgc
     2401 acgatgccaa ggaaagcgaa ggcgcgccac ctcatgaagt agtggcgaac ctcagcgaag
     2461 cgctcgtact gctacgatcc cgctttgcgc tgacagagcc tgatccaccc tacgagcatc
     2521 cttagcacat ggccaaacat tcttattgct gataatcacg ctgatcttgc tctcgggcaa
     2581 gcggctcgcc acattttgca gataaccgct caatgccaaa acagaccgct ctgatagtgg
     2641 gaattactgg cccggacggt gcttatcttg cacgcttcct gctcgataag ggttatgacg
     2701 tttacggtac gtctcgaaac atcggtctcg cacgtaccga tggtcttcga g
//
LOCUS       DATNOI010000672         3499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1806995,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000672 DATNOI010000000
VERSION     DATNOI010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806995"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VN968_26915"
     CDS             <1..311
                     /locus_tag="VN968_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32896.1"
                     /translation="HTWHSGADCINPAVAIGVRASGEKAWFGWPNAPEVETQVTAWYE
                     AKTLDEEKAAIGRLNKAALDSVVYAPTGFFLGYQAWRKNVSGVVKGPLPFFWGVAKVA
                     "
     gene            436..2037
                     /locus_tag="VN968_26920"
     CDS             436..2037
                     /locus_tag="VN968_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32897.1"
                     /translation="MNRRAFLKSVAGAGVLTTAGALAIPAISQRAAARVLRFVPQADL
                     ANFDPIWNTQYVVRNGAALVWDMLYGVDDKLQPRRQMVESEEASSDGLVWTFRLRGGL
                     KFHDGEPVLAKDVVASLARWQARDNMGLMTKEIEQELVAVDDRTFRWVLKKPYPKLLV
                     ALGKVGTPSCFIMPARIAATDPFKPIGDYIGSGPMRFVRNEWVPGAKAVFERFADYAP
                     RQEPNSWLSGGKHMLLERIEWIVMPDPATASAALQNGEVDWWETPIADIVPVLKKNRN
                     VMVDIADPLGNIGSFRMNHLHPPFNDVRARRAILTAMNQEDYMRAVVGDDALWKPLPG
                     FFTPGTPLYSEDGGEILKGPRRLDAAKRLLADSGYSGQPVTCVVAQDLQITKAQGEVT
                     ADLLRKLGVNVDFAAVDWGTNAARRAQKSPPGQGGWQMFHTWHAGAVCIDPSSYAAIR
                     ANGDKAWFGWPTSAKVESEITAWYDARNLDEEKAAIARLNKAALEDVVYAPTGFFLAY
                     QAWRKNVDGIVKGPLPFFWGVSKAA"
     gene            2235..3176
                     /locus_tag="VN968_26925"
     CDS             2235..3176
                     /locus_tag="VN968_26925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL32898.1"
                     /translation="MLSYTIRRILATIPVMVVVALFVFSLLYIAPGDPAAIIAGDQAT
                     PADVERIRQSLGLDRPFLIRFGEWLWQILHGDLGTSIFTNLPVAAMIAQRVEPTLSLM
                     AVTLVIAICLAVPMGVVAAWQADTLIDRAIMAFAVLGFSVPVFVVGYLLAYVFALELD
                     WLPVQGYTQISEGLWPWFENLILPALALGCVYIALIARITRASMLEVLQQDFIRTARA
                     KGVGQTRILFVHALKNAAVPIVTVIGIGVALLIGGAVVTETVFAIPGIGRLTVDAILR
                     RDYPVIQGVVLMFSFLYVLVNLLIDLLYTVLDPRIRY"
     gene            3173..>3499
                     /locus_tag="VN968_26930"
     CDS             3173..>3499
                     /locus_tag="VN968_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL32899.1"
                     /translation="MTDATITTAPELIRAAPELPDLLPPIKVRGGILGFARRHPAIAI
                     GGALVLAMVLIAVFAPYLGTVDPTALAPTRRTREPSAAFWFGTDMLGRDIYSRVLYGA
                     RVSLTVG"
BASE COUNT          582 a         1141 c         1122 g          654 t
ORIGIN      
        1 tccatacctg gcattccggc gccgactgca tcaatccggc ggtcgccatc ggggtgcgcg
       61 ccagcggcga gaaggcgtgg ttcggctggc cgaacgcgcc ggaggtcgaa acgcaggtga
      121 cggcttggta tgaggccaag accttggacg aggagaaagc ggcgataggg cggctcaaca
      181 aggccgcgct cgacagtgtg gtctacgcgc cgaccggctt tttcctcggc tatcaggcct
      241 ggcgcaagaa cgtgagcggc gtggtgaagg ggccgctgcc attcttctgg ggcgtcgcga
      301 aggtcgcctg agccgcgccg gtgcggcctc aagggcgtct acgcccgtct tcgcgggcta
      361 tggcgtaacc ggcgacacgc gcgcgccggt catgtaccct acctgatgac ttgccgtgac
      421 cgggatgggc gcttcatgaa tcgaagggcg ttcctgaaat cggtagccgg cgccggcgtc
      481 ctcaccacag ctggcgcgct tgccattccc gcgatctcgc agcgcgccgc cgcgcgcgtg
      541 ttgcgcttcg tgccgcaggc cgatctcgcc aatttcgatc cgatctggaa cacccagtac
      601 gtcgtgcgca acggcgccgc gttggtgtgg gacatgcttt acggcgtcga cgacaagctt
      661 caaccgcggc gccagatggt cgagagcgag gaagcctcgt cggacggact cgtttggacg
      721 ttccgtttgc gaggagggct caagtttcat gatggcgagc ccgtgctcgc gaaggacgtc
      781 gtggcgagtc tggcgcgatg gcaagcgcgc gacaacatgg gtctgatgac caaggagatc
      841 gagcaagaac ttgtcgccgt cgacgatcgc accttccgct gggtgctgaa aaaaccttat
      901 ccgaagctct tggtcgcgct cggcaaggtc ggaacgccgt cctgcttcat catgccggcg
      961 cggattgcgg cgaccgatcc gttcaaaccg atcggcgact atatcggcag cggaccgatg
     1021 cggttcgtgc gcaacgagtg ggttcccggc gccaaggcgg tgttcgaacg gttcgccgat
     1081 tatgcgccgc gccaggagcc gaattcttgg ctctccggcg gcaagcacat gctgctcgag
     1141 cgcatcgagt ggatcgtgat gcccgacccc gccaccgcct cggccgcgct tcagaacggc
     1201 gaagtggatt ggtgggaaac cccgatagcc gacatcgtgc cggtgctgaa aaagaaccgc
     1261 aacgtcatgg tcgatattgc cgatccgctc ggcaacatcg gctcgtttcg catgaaccac
     1321 ctgcatccgc ccttcaacga cgtgcgggcg cgacgcgcga tcctcacggc gatgaaccag
     1381 gaagattaca tgcgcgcggt cgtaggtgac gacgccctgt ggaaaccgct gcccggattc
     1441 tttacgccgg gcacgccgct ctacagcgag gacggcggcg aaattctcaa aggtccgcgc
     1501 cgtctcgatg ccgccaaacg attgctggcg gacagcggct attccggtca gccggtcacc
     1561 tgcgtggttg cgcaggattt gcaaatcacc aaagctcagg gcgaggtcac tgccgacctg
     1621 ctcaggaagc tcggcgtgaa cgtcgatttc gctgccgtcg actggggcac gaacgcggcg
     1681 cgtcgagcgc agaaatcgcc gccgggacaa ggcggctggc agatgttcca tacttggcat
     1741 gccggcgcgg tttgtatcga cccgtcgtcc tatgcggcga tccgcgccaa cggcgacaag
     1801 gcgtggttcg gctggccgac cagtgcgaag gtcgaaagcg agatcaccgc ctggtacgac
     1861 gcaagaaacc tcgacgagga aaaagccgcc atcgcgcggt tgaacaaggc cgcactcgag
     1921 gacgtcgtct acgcgccgac cggcttcttc ctggcctatc aggcctggcg caagaatgtc
     1981 gacgggatcg tgaaggggcc gctaccattc ttctggggcg tgagcaaagc agcttgaatg
     2041 cgttcccagt cctgcgccca cggcccacgc ggatgccaaa ctttgccctt cgcaacgcga
     2101 acgacccctt gccgcgccga atgactcgac ttgacgcggt gtcttatcgg tcgtcgctgg
     2161 gccgtgtctc agttgagacg ccactcatcg ccggcagatc ggcaaaatgg catgaaacct
     2221 gctagcatcg gcacatgctg agctacacga tccgtcgcat cctggccacc atcccggtga
     2281 tggtcgtggt tgcgctgttc gtgttcagcc tgctctacat tgcgccgggc gacccggcgg
     2341 ctatcatcgc cggcgatcaa gcgacgccgg ctgacgtcga gcgcattcgc cagagccttg
     2401 gcctcgaccg accgttcctg atccggttcg gtgagtggtt gtggcaaatt cttcacggcg
     2461 atctgggcac gtcgatcttc accaacctgc cggtcgccgc gatgatcgcc cagcgcgtcg
     2521 agccgaccct gtcgctgatg gcggtgacgc tggtgattgc catctgcctc gccgtgccca
     2581 tgggcgtggt cgcagcctgg caagccgaca ccttgatcga ccgcgccatc atggcgtttg
     2641 cggtgctcgg cttctcggtg ccggtcttcg tcgtgggcta tctgctggcc tatgtcttcg
     2701 cactcgagct cgattggctg ccggtgcagg gctatacgca aatctccgaa ggcctgtggc
     2761 cgtggttcga aaatctgatc ctgcctgcgc tcgcgctcgg ctgcgtctat atcgcgctca
     2821 tcgcccgcat cacgcgcgcc tccatgctgg aggtgctgca gcaggatttc atccgcaccg
     2881 cgcgcgccaa gggagtggga cagacccgca tcctgttcgt gcacgcgctc aagaacgccg
     2941 cggtgccgat cgtaacggtc atcggcattg gcgtcgcgct cctgatcggc ggcgcagtgg
     3001 tgacggagac ggtgtttgcg atccccggca tcggccggtt gaccgtcgat gccatcctgc
     3061 gccgcgacta cccggtgatc cagggcgtcg tgctgatgtt cagcttcctc tacgtgctcg
     3121 tcaatctcct catcgatctc ctctacaccg tgctcgaccc gaggatccgg tattgaccga
     3181 cgccactatc accaccgcgc ccgagctcat ccgcgccgcg cccgagctgc ccgacctgct
     3241 gccgccgatc aaggtccgcg gcggcatcct cggctttgcg cgccgacatc cggcgatcgc
     3301 catcggcggc gcactggtgc tggcgatggt gctgatcgcc gtgttcgcgc cctatctggg
     3361 cacggtcgac cccactgcgc tggcgccgac gcggcgcacc cgcgagccgt ccgccgcatt
     3421 ctggttcggc accgacatgc tgggacgcga catctactcg cgcgtgctct atggcgcacg
     3481 ggtgtcgctc accgtcggc
//
LOCUS       DATNOI010000673         2760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2358644,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000673 DATNOI010000000
VERSION     DATNOI010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2358644"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VN968_26935"
     CDS             <1..1006
                     /locus_tag="VN968_26935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HXL32900.1"
                     /translation="SKTKTGAWSTSAQVLDELAEQGHEFPKKILEWRQVSKLKSTYTD
                     ALPTYVHPQTHRVHTTYALAATTTGRLSSNEPNLQNIPVRTEDGRKIRRAFIATPGHK
                     LVSADYSQIELRLLAEIADIPVLKQAFRDKLDIHAMTASEMFGVPIKDMPSEVRRRAK
                     AINFGIIYGISAFGLANQLGIAREEASAYIKKYFERFPGIRAYMDETRDFCRKHGYVE
                     TLFGRKCHYPDIKASNASIRSFNERAAINARLQGTAADIIRRAMIRMEDALAEKKLSA
                     QMLLQVHDELIFEVPDDEVAATLPVVQHTMQDAPFPAVLLSLPLQVDARAANNWDEAH
                     "
     gene            1201..1587
                     /locus_tag="VN968_26940"
     CDS             1201..1587
                     /locus_tag="VN968_26940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HXL32901.1"
                     /translation="MTPVEIYNAEHARLIAEENWIIDGLGRFDSIPERLSRATDIVVV
                     DMPLWMHFWLAAERQIRWSTGAIENPPAGLTKMPPTEVLFRTIWQVDQNWMPEIRRLV
                     AFEKQRGKQVFRLSSIAELNEFENRS"
     gene            1891..2526
                     /locus_tag="VN968_26945"
     CDS             1891..2526
                     /locus_tag="VN968_26945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HXL32902.1"
                     /translation="MPHLTSLLGFAMVSLGMVLTPGPNMIYLISRSITQGHIAGLISL
                     GGVALGFVFYMLCAAFGITALLFAVPYAYDALRLAGAAYLLWLAWQAVRPNGRSPFQV
                     NKLALDGPRKLFAMGFVTNLLNPKIAMLYLALLPQFIDPVQGSVLTQSLALGFIQIAI
                     SVSVNALIALTAGSIALFLGTRPTWLLVQRWLMGTVLAGLAMKIAFEAKRA"
BASE COUNT          554 a          865 c          824 g          517 t
ORIGIN      
        1 ctcaaagacc aagaccggcg cgtggtcgac ctccgcgcag gttctcgatg aactcgccga
       61 gcagggccac gaatttccaa agaagatcct cgaatggcgg caggtctcga agctgaaatc
      121 gacctatacc gacgcgctgc ctacctacgt gcatccgcag acccatcgcg tccacaccac
      181 ctacgcgctg gcggcgacca ccaccggccg gctgtcgtcg aacgagccca acctgcagaa
      241 cattccggtg cgcaccgagg acggccgcaa aatccggcgc gcctttatcg cgacgcccgg
      301 ccacaagctg gtgtcggcgg attattcgca gatcgagttg cggctattgg ctgaaatcgc
      361 cgatattccc gtgctcaagc aggccttccg cgacaagctc gacattcacg ccatgacggc
      421 gtcggaaatg ttcggcgtgc cgatcaagga catgccgagc gaagtgcgcc gccgcgccaa
      481 ggcgatcaat ttcggcatca tctacggcat ctcggcgttc ggcctcgcca accagctcgg
      541 tatcgccaga gaggaagcct ccgcctacat caagaaatac tttgagcgtt ttcccggcat
      601 tcgcgcctac atggacgaga cgcgcgattt ctgccgcaag catggctatg tcgaaaccct
      661 gttcggccgg aaatgtcatt atcccgacat caaggcctct aacgcttcca tccggtcttt
      721 caacgagcgc gccgcgatca acgcccgcct tcagggcacg gcagccgaca tcatccgccg
      781 cgccatgatc cggatggaag atgcgctggc ggagaagaag ctctcggcgc agatgctgtt
      841 gcaggttcac gacgaactga ttttcgaggt acccgacgac gaggtcgcgg cgaccttgcc
      901 ggtggtgcag cacaccatgc aggatgcgcc attcccggcc gtgctgctct cgctgccgct
      961 gcaggtcgat gcccgggcgg cgaacaactg ggacgaggcg cattaagtta ttaaagcgca
     1021 ttctttcttc acctctccct gcccacgggg agagggagaa gacggcagac cgccatgaag
     1081 agagcagtca tcatcggcaa cagcggcggt ggcaaatcaa cgctggcgcg gcggctaggt
     1141 tcggcatggc gatctcctca cgtcgaaatt gacggtctgc tgtggcaacc ggggtgggaa
     1201 ttgacgccgg ttgaaatcta caacgccgag cacgcgcgcc tgatcgccga agaaaactgg
     1261 atcatcgacg ggctgggacg gtttgactca atcccggaga gactatcgcg agcgaccgat
     1321 atcgttgttg tcgacatgcc gctgtggatg catttctggc tagctgccga acggcagata
     1381 cggtggtcga caggtgccat cgaaaatcca ccggcgggcc ttacaaaaat gccgcccacg
     1441 gaggtgctgt tccgaacgat ctggcaggtc gatcaaaact ggatgccgga aattcgccgg
     1501 ctcgtcgcgt ttgaaaagca acgcggaaaa caggtttttc ggctcagctc gattgccgaa
     1561 ctcaacgagt tcgaaaatcg gtcgtgagcg tcaccgacga tcaaggtgcg cgctttcttc
     1621 acctctcccg cttgcggggg aggtcgacgc gctcggcaga gcgcggcggg tgggggaaga
     1681 ggcccgcacg agcacgatca aagagccccc accccaaccc tcccccgcaa gcgggagagg
     1741 gagcagacag caatcgcctc acgcgctcct gaaatattcg agggagcgca ccgccgcccc
     1801 tgatgaattg ctctcggtgc aatttacgaa atgacatcgc gatattgcgc gggctagaaa
     1861 cgcgcaaatt ccttcccgcg agtcctcccc atgccccacc tcacctccct gctcggcttc
     1921 gccatggtct cgctcggcat ggtgctgacg cccgggccga acatgatcta tctgatctcg
     1981 cgctcgatca cgcagggcca catcgccggc ctgatctcgc tcggcggtgt cgcgctcggc
     2041 tttgtgttct acatgctgtg cgccgcattc ggcatcacgg cgctgttgtt cgccgttcct
     2101 tacgcctatg acgcgctgcg gctcgcgggt gccgcgtatc tgctgtggct ggcgtggcag
     2161 gccgtgaggc cgaacggacg ctcgccgttt caggtgaaca agcttgccct cgacggaccg
     2221 cgcaaattgt tcgcgatggg ttttgtcacc aatctgctga acccaaagat cgcgatgctg
     2281 tatctggcgc tgctgccgca gttcatcgat ccggttcagg gcagcgtgct gacgcagtcg
     2341 ctggcgcttg gatttatcca gatcgcgatc agcgtcagcg tcaacgcgct gatcgcactc
     2401 accgccggat cgatcgcgtt gtttctcggc acgcggccga catggttatt ggtgcagcgc
     2461 tggctgatgg gcacggtgct cgccggccta gccatgaaga tcgcgttcga ggcgaagcgg
     2521 gcgtgaaggc aaagccgacg cagagcagtt gacggtcatc gcccggctag accggccgcc
     2581 cagcatgcac cgggatcaat gagtcgggac gcgccgccta tcgtgcgaac caacatccgg
     2641 agcgcgctca ttgttgacat accgagccag gaacgcctgc cataaacgct gtccaaggcg
     2701 atcatattgc cgcatttgct cgatgagccg atatcgatcg tcggtggcaa gaagggcctt
//
LOCUS       DATNOI010000674        11320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_905629,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000674 DATNOI010000000
VERSION     DATNOI010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11320
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905629"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VN968_26950"
     CDS             <1..666
                     /locus_tag="VN968_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HXL32903.1"
                     /translation="VDVQICTERDDVLLTAAGGQCIRFPVTDVRVFQGRTSMGVRGIA
                     LAKADKVISLAILRHVDADAEERAAYLRRANAVRRGGADEETGTDSEEASGAIELGEQ
                     RYVEMSANEQFVLTISENGYGKRSSSFEYRTTGRGGKGIVAMSVNDRNGKLVASFPVE
                     DSDQIMLVTDRGQLIRCPVEGIRIAGRSTQGVIVFDTAEDEHVVSVEHIGEDTENGNG
                     NGG"
     gene            complement(754..2661)
                     /locus_tag="VN968_26955"
     CDS             complement(754..2661)
                     /locus_tag="VN968_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HXL32904.1"
                     /translation="MTATPVAAPIHSNALPLTATLGALGVVYGDIGTSPLYALKEAAK
                     AAAHGGPLVPEAILGVVSLILWALILIISVKYALLILRADNRGEGGIVALLALLHARN
                     ARPGTWRAQLLVVGLIGAALLYGDGAITPAISVLSAIEGLKVDAPSLAPAVVPLTIVI
                     LIGLFIVQKKGAGFIGNIFGPVMLAWFLVIGLLGVHGIVQAPGVLAAVSPLYAFNFLI
                     HQDFHISFAVLGAAFLAVTGGEAMYADMGHFGRVPIRLAWFGAALPGLVLNYFGQAGL
                     LVTDPTALDHPFYQLAPDWLHYPLVAFATVATVIASQAIISGVFSLTQQSIQLGFLPR
                     MHITHTTSHAMGQIYVPLVNWLLAAATLGAVIGFGTSDALAGAYGIAVSLLMAITTLL
                     AALVAIQWGYSPIIVIAVNGFFFAIDCIFFAANSTKLFEGGWFPLLLAAVIAFVMLTW
                     RGGVKLVERARSALRQPEEDLIETAVNRCHARLSGTAVFLASAPNGVPLALTQFVKHN
                     HVLHQRVLLVTVVIEESPRIADEDRAEVTEVIEGITRVILHYGFMQYPTIFEGLKLAC
                     AQGKLPGIDLTNITYYIGRETIIPREDIPGMWVWRESLFAFLQRNAERTAAFFDVPAR
                     QVVEFGTEIEI"
     gene            complement(2788..3045)
                     /locus_tag="VN968_26960"
     CDS             complement(2788..3045)
                     /locus_tag="VN968_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32905.1"
                     /translation="MIKAVSAIAVAAFVAAALSILPGFAPQVEASVPQALAKGDRLDI
                     HTAGGNCAEQAWPNFEASCLRATGSSIMIRQARLVTAHRIP"
     gene            3312..3809
                     /gene="coaD"
                     /locus_tag="VN968_26965"
     CDS             3312..3809
                     /gene="coaD"
                     /locus_tag="VN968_26965"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HXL32906.1"
                     /translation="MPRIALYPGSFDPVTNGHLDVVRQAVGLCDRLIVAIGVHPGKKP
                     LFSVEERLDMVRQVFGPIAAKAGCAFESTTYDNLTTTAAQKNGATILIRGLRDGTDLD
                     YEMQMAGMNEVMAPDVQTVFVPASVSVRPITATLVRQIASMGGDVSAFVPQSVAASLK
                     TRFAG"
     gene            3900..4463
                     /locus_tag="VN968_26970"
     CDS             3900..4463
                     /locus_tag="VN968_26970"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXL32907.1"
                     /translation="MIRIFAFVAALICAAPAIAQPLPANLDKQNAIVIDTSKGRIVFK
                     LRTDLAPQHAERIKQLAREGYYNNVPFHRVMDGFMAQTGDGQNFNGTGGSKYPNLKAE
                     FSNVPFKRGIVGMARRGDSVDTANSQFFFMFADAPSLNGQYTVIGEVVSGMDVIDKLK
                     KAPAGSSSGSVADPDKMVKVQVASDIK"
     gene            4514..4909
                     /locus_tag="VN968_26975"
     CDS             4514..4909
                     /locus_tag="VN968_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32908.1"
                     /translation="MFAFSSWTSQAETAQVNSIQDVIARLRTCWKPPPAGRARPGIDI
                     TVIVSFNRNGDILGHPRIAYESEQATDNDRLMYRIAVMEALQRCTPMPFTEGMAGAVA
                     GRPFHVQFQTRKRPPQPVEKRAWLIPKIL"
     gene            4885..5349
                     /locus_tag="VN968_26980"
     CDS             4885..5349
                     /locus_tag="VN968_26980"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXL32909.1"
                     /translation="MADTENTLILETTRGPVTIEMRPDLAPGHVARIKELVREGFYDG
                     IVFHRVIEGFMAQTGCPQGTGTGGSGKKLKAEFNSEPHVRGTASMARAASPDSADSQF
                     FICFDDASFLNNQYTVWGKVTAGMENVDKIKRGEPVQNPDKIVKARMAADAA"
     gene            5457..6536
                     /gene="queA"
                     /locus_tag="VN968_26985"
     CDS             5457..6536
                     /gene="queA"
                     /locus_tag="VN968_26985"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HXL32910.1"
                     /translation="MRTDLFDFDLPAGHVALRPASPRDSARMLIVQPDGVLLDRVVAD
                     LPDWLEPGDQLVVNDTKVIAAKLSGRRIGRDTEPKIEATLIKRLDGSRWQALAKPAKK
                     LSEGDVVRFGNEGKVCLLGHLDAEVEHKGEDGEVTLSFSFHGPMLDQAIGDLGAPPLP
                     PYIASRRAPDERDASDYQTMFAVNEGAVAAPTAGLHFTQALETALRERGVEIHRVTLH
                     VGAGTFLPVKAEDTSAHKMHAEWGTISRTTADALNAARAKGGRVVAVGTTALRLLESA
                     AAEDGAIEPFTGETAIFIAPGYRFRAVDILMTNFHLPRSTLFMLVSAFSGLDTMKRAY
                     AHAIGNGYRFYSYGDACLLFRDKST"
     gene            6536..7669
                     /gene="tgt"
                     /locus_tag="VN968_26990"
     CDS             6536..7669
                     /gene="tgt"
                     /locus_tag="VN968_26990"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HXL32911.1"
                     /translation="MTSANHFELLGRDGKARIGRLTTPHGAVRTPAFIPIGTGGAMKG
                     LHWREVRDAGTDIVLGNTYHLMLRPGAERIAALGGLQAFTGWNGPMLTDSGGFQVMSL
                     AELRKVRESAVTFRSHIDGAMVELSPERSIEVQRLLGSDIAMQMDECMRLPAERSDIE
                     RAMQLSLRWAERSRRAFEAAPDGYMVFGIAQGGDVPELRQASARGLLEIGFHGYAIGG
                     LAVGEPQAVMLAMIDEVAPILPQDRPRYLMGVGTPEDILEAVARGIDMFDCVLPTRNG
                     RHGMAFTRFGQINLRNARHADDPRPLDEESSWPSTRSYARAYLHHLVRSGETLGAMLL
                     SEINVAYYQRLMQDIRDAISKGIFESFCERTRAEWARGDIPPR"
     gene            complement(7692..7907)
                     /locus_tag="VN968_26995"
     CDS             complement(7692..7907)
                     /locus_tag="VN968_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32912.1"
                     /translation="MPALAEVMSTVAPKGDPRVCDLPTCPVCADSMVAAEASAFVSDN
                     VVSYLWTCDTCGYGFVTKHSLRRFACN"
     gene            8128..9105
                     /gene="cysK"
                     /locus_tag="VN968_27000"
     CDS             8128..9105
                     /gene="cysK"
                     /locus_tag="VN968_27000"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HXL32913.1"
                     /translation="MDASSATSAVHRPGRGRVFDSIVDAIGDTPIVRLRKMPQQHGAN
                     ATILAKLEYFSPAASVKDRIGAAMVIAMEKAGLINADTVLIEPTSGNTGIALAFVAAS
                     RGYRLKLVMPESMSIERRKMLAFLGAEIILTPAAQGMKGSIATAEELVRTTPNAVMPQ
                     QFKNLANPEIHRRTTAEEIWNDTGGNIDIFVAGVGTGGTITGVGQVLKPRKPSLRIVA
                     VEPEESPVLSGGQHSPHKIQGIGAGFIPDILDRSVIDEIVKVNSTTAIETSRALARNE
                     GIAGGISSGAAIAAALAIGKRPENAGKTILAVVPSFAERYLSTALFEGI"
     gene            9108..9347
                     /locus_tag="VN968_27005"
     CDS             9108..9347
                     /locus_tag="VN968_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HXL32914.1"
                     /translation="MPDQPRRPRTLNDARTEAEAAFKRTTTKVPEAPPKTAALPSVKE
                     LVSLRIDQDVLEHFQEGGPGWQDRINDALRKAAGK"
     gene            9362..9682
                     /locus_tag="VN968_27010"
     CDS             9362..9682
                     /locus_tag="VN968_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32915.1"
                     /translation="MRSHLARIWFALLAGAQMTPASAESVPFNCVILPGSELASIMMT
                     NSLPGDASCMVTCKFSTTKYDNNPQITCAKPIPAGKEVEMCRLSSGGDKMVKLMEGHA
                     ECTR"
     gene            9869..10267
                     /locus_tag="VN968_27015"
     CDS             9869..10267
                     /locus_tag="VN968_27015"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXL32916.1"
                     /translation="MRILQPADWPKPRGFSHGVTLDGPGQWIVLAGQTGGDEKGDYQP
                     DMANQVGTALRRIVKLLEEAGAGPEHIVRLTWYLTSRSEYEAAGAAIGAAWRETLGRN
                     FPPSTLLYISGLVDERAKVEIEVTAFGPAG"
     gene            10277..10477
                     /locus_tag="VN968_27020"
     CDS             10277..10477
                     /locus_tag="VN968_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32917.1"
                     /translation="MTKRSTSADFVTAFATGWPENQPEIMVLSLTTQKGVQDFALSKE
                     QALLIAKTMKQTAAQLAKPKQA"
     gene            complement(10503..11066)
                     /locus_tag="VN968_27025"
     CDS             complement(10503..11066)
                     /locus_tag="VN968_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32918.1"
                     /translation="MGHLQAYRAAVGGKHPGNKTGLTTGSFAGVTRLIFIVTALFVAS
                     ASGSASYAQSGPFAGMAGNWSGGGTVTLDDGSSERIRCRATYAVGAGGTGLNQSLTCA
                     SDSYRFILSSNVVAQGNALSGTWSESSRGINGTLEGRGSNGNFQVVANAPGFTANLSL
                     ATRGNRQSVVITAASQFRGANIALTRH"
     gene            complement(11011..>11320)
                     /locus_tag="VN968_27030"
     CDS             complement(11011..>11320)
                     /locus_tag="VN968_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32919.1"
                     /translation="LTVLAAAPAVAQTPYDGLWNVTIVTKAGSCEPTTRSTLTVSDGK
                     VSAPGASVSGSIGREGLVRVSIAGAYANGQLNGNSGSGKWNGASAGVPCSGRWEASRQ
                     "
BASE COUNT         2259 a         3483 c         3614 g         1964 t
ORIGIN      
        1 gtcgacgtgc agatctgcac cgagcgcgac gacgtgctgc tgaccgccgc cggcggccag
       61 tgcatccgct tccccgtcac cgatgtgcgg gtgttccagg gccgcacctc gatgggcgtg
      121 cgcggcatcg cgttggccaa agccgacaag gtgatctcgc tcgccatcct gcggcacgtg
      181 gatgctgatg ccgaggagcg cgccgcctat ctgcgccgtg ccaatgcggt gcggcgtggc
      241 ggcgccgatg aggagaccgg taccgacagc gaggaagcgt ccggcgccat cgaactcggc
      301 gagcagcgct atgtcgaaat gtcggccaac gagcaattcg tgctgaccat cagcgaaaac
      361 ggctatggca agcgctcgtc gtcgttcgag taccggacca cggggcgcgg cggcaagggc
      421 atcgtcgcga tgtcggtaaa cgaccgcaac ggcaagctgg tggcgtcatt cccggtcgag
      481 gactctgacc agatcatgct tgtgaccgac aggggccagc tgatccgctg cccggtcgag
      541 ggcatccgca tcgcgggccg ctcgacccag ggcgtcatcg tgttcgacac cgccgaggac
      601 gagcatgtgg tgtcggtcga gcacatcggc gaagacaccg aaaacggcaa tggcaacggc
      661 ggctgagtca ttccgcgaca tgcgtctagg cctgaacccg gaatctcgag gttccgggtt
      721 ctacgctgag cgtcgccgcg gaatggcgaa tacctaaatc tcgatctcgg tgccgaactc
      781 gacgacctgg cgcgcgggca cgtcgaagaa tgccgccgtc cgctcggcgt tgcgctgcag
      841 aaacgcaaac agcgactcgc gccacaccca catgcccgga atatcctcgc ggggaatgat
      901 ggtctcgcgg ccgatgtaat aagtgatatt ggtgagatcg attccgggca gtttgccctg
      961 cgcgcaagcg agcttcagac cctcgaaaat cgtcggatac tgcatgaaac catagtgcag
     1021 gatgacgcgg gtgatgccct cgatcacctc ggtgacctcc gcccgatcct cgtcggcgat
     1081 gcgaggcgac tcctcgatca cgacggtaac gagcagcacg cgctggtgca gaacgtggtt
     1141 gtgcttgacg aattgcgtta acgcgagagg gacgccgtta ggcgcggatg ccagaaacac
     1201 cgcagtgccc gacagtctgg cgtggcacct gttgacggcg gtctcgatca gatcctcttc
     1261 gggctggcgc aaagcgctgc gtgcccgttc gaccagcttg acgccgcctc gccaggtcag
     1321 catcacgaac gcaatgacgg ccgcgagcag cagtggaaac cagccaccct cgaacagttt
     1381 ggtggaattc gcggcaaaga agatgcagtc gatggcgaag aagaagccgt tgacggcgat
     1441 cacgatgatc ggcgaatagc cccattggat cgcgaccagg gcagccagca gcgtggtgat
     1501 ggccatcagc agcgacacgg caattccgta tgcgcctgcg agcgcatccg acgtgccaaa
     1561 gccgatgacg gcgccaagcg ttgccgccgc cagcagccag ttcaccagcg gcacgtagat
     1621 ctggcccatg gcgtggctgg tggtgtgggt gatgtgcatg cgcggcagga agccgagctg
     1681 gatcgactgt tgcgtcagtg aaaacacgcc ggagataatt gcctgcgagg caatgacggt
     1741 ggctacggtg gcgaacgcga ccagcgggta atgcagccag tcgggcgcga gctgatagaa
     1801 cggatggtcg agcgcggtgg ggtcagtaac cagcagcccg gcctgtccga aatagttgag
     1861 gacgaggccg ggaagggccg ccccgaacca tgcgagccgg atcggcactc ggccgaaatg
     1921 acccatgtcg gcatacatcg cttcgccgcc cgttaccgcg agaaaggcag cgcccagcac
     1981 cgcgaagctg atgtggaaat cctggtggat caggaaattg aaggcataaa gcgggctgac
     2041 tgcggccagc acgccgggcg cttgcacgat gccgtggacg ccgagcaggc cgatcaccag
     2101 gaaccaggcc agcatgacag gcccgaagat attgccgatg aagcccgcgc cctttttctg
     2161 aacgatgaag agcccgatca ggatgacgat ggttagcggc accacggctg gtgccagcga
     2221 tggcgcatcg accttcaagc cttcgatcgc gctcagcacc gaaatcgccg gcgtgatggc
     2281 gccgtcgcca tagagcagcg ccgcaccgat aaggccgacc accagaagct gcgcacgcca
     2341 cgttcccggc cgggcgttgc gggcgtggag cagcgccagc agcgcgacga tgccgccttc
     2401 gccgcggttg tcggcacgca agatcaacag cgcatatttg acagaaataa tcagaattag
     2461 cgcccagagg atcagcgaca ccacgccaag gatcgcctcg gggacgagcg gcccgccatg
     2521 agcggccgcc ttggccgctt ccttcagggc atagagagga ctggtgccga tgtcgccata
     2581 gacgacgccc agcgctccga gcgttgccgt caatggaagt gcgttggaat ggattggagc
     2641 tgcgactggt gtagcggtca ttggctcccc caaaaccgtc gcgcccgccg gctcagtggc
     2701 cggccaaggc ggatcgattg ccagtctgcg acggcgcgcc gcaaattacc atggggttag
     2761 tttggtgcgg aaggaagacc ttgcggctta tggaatgcgg tgggccgtta ccaaccgggc
     2821 ttgccggatc atgatgctgg agccggtggc gcgcaggcag gacgcctcga aattgggcca
     2881 ggcctgctcc gcgcagttgc cgcccgccgt gtggatgtcg agccggtcgc ctttggcgag
     2941 cgcttgcgga acgctggctt cgacctgcgg agcaaaaccg ggaagaatgg aaagagcggc
     3001 agcgacaaac gcagccacag cgatggctga aacagccttg atcatgatgt ccccctgcat
     3061 cgggcctttg cggcccgtcg ttggcttggt taattgctac ccagcgcctg tttccggatg
     3121 tcttcgcaaa ggccgaaaat ggtttcgtcc cgcccgagtt ttgtttcgtc gccggtcacg
     3181 gcacgaaaca tggccgggct gccttgtggg acgccgcgag gtgccaggcg gttcaagaca
     3241 agagggcggc ttttgccgca gcggcaatgg gctcggcttg cggggcagcc cgggccgggg
     3301 tagacgggtt tatgccccgc atcgcgcttt atcccggctc attcgacccc gtcaccaatg
     3361 gccatctgga tgtggtcagg caggccgtcg gcctttgtga ccggcttatt gtcgcaatcg
     3421 gggtccatcc cggcaaaaaa ccgctgtttt ccgtcgaaga acgcctcgat atggtccggc
     3481 aggtatttgg gcctatcgct gcgaaggctg gttgcgcctt tgaaagtaca acctacgaca
     3541 acctcaccac tacggcggcg cagaagaacg gcgcgaccat cctgattcgg ggcttacgcg
     3601 acgggaccga cctcgactac gagatgcaga tggccggcat gaacgaagtg atggctcccg
     3661 acgtccagac ggtgttcgtt ccagcttcgg tttcggtccg cccgatcacc gccacactgg
     3721 tgcgccaaat cgcaagcatg ggcggcgacg tctcagcgtt cgtgccgcaa tcggtcgccg
     3781 ccagcctcaa gaccagattc gccggctaga gcgcgtattc cgcaaaagtg ggaaccggtt
     3841 ttgcgataag aatacgcgca gctctaaagt gctctatcct tccctgatcc ggagttttca
     3901 tgatccgaat tttcgcattc gttgccgcgc tgatttgcgc ggcccccgcg atcgcgcagc
     3961 cgcttcctgc aaatctcgac aagcagaatg cgatcgtgat cgacaccagc aagggccgca
     4021 tcgtcttcaa actgcgaacc gacctggcgc cccagcacgc cgagcggatc aagcaactgg
     4081 cgcgcgaagg ctactacaac aacgtgccgt ttcaccgcgt gatggacggc ttcatggcgc
     4141 agaccggcga cggccagaat ttcaacggca ccggcggttc gaaatacccc aatctgaagg
     4201 cggaattttc caacgtgccg ttcaagcgcg gcatcgtcgg catggcgcgg cgcggcgaca
     4261 gcgtcgacac cgcgaactcg cagttcttct tcatgttcgc cgacgccccc agcctgaacg
     4321 gacaatacac cgtcatcggc gaagtggtgt cgggcatgga cgtgatagat aaactgaaga
     4381 aggcccctgc ggggtcttcc agcggcagcg tggccgaccc cgacaagatg gtgaaggtac
     4441 aagtcgcatc cgacatcaag tgagcgcggt ggcttggcgt ggcaaaccgc tgatctggct
     4501 cgcaggagcg gcattgttcg ccttctcgag ttggacctcg caggccgaga cggcgcaggt
     4561 caattccatc caggacgtga tcgcgagatt gaggacctgc tggaagccgc cgccggcggg
     4621 acgcgcccgt ccgggcatcg acatcaccgt gatcgtaagc ttcaaccgca atggcgacat
     4681 cctcggtcat ccgaggattg cctatgaatc ggaacaggcc acggacaatg atcggctgat
     4741 gtatcgcatc gccgtgatgg aggcgttgca acgctgcacg ccgatgccat ttactgaagg
     4801 aatggccggc gccgtcgccg gccggccgtt ccatgtccag ttccagacta ggaaacgtcc
     4861 accccaaccc gtggagaagc gagcatggct gataccgaaa atactttgat cctcgaaacc
     4921 acgcgggggc cggtgaccat cgaaatgcgc ccggaccttg cacctggcca cgtcgcacgg
     4981 atcaaggaac tggtgcgcga gggattttac gacggcatcg tgttccatcg cgtgatcgaa
     5041 ggtttcatgg cgcaaaccgg ctgtccgcag ggcaccggca ccggcggctc cggcaagaag
     5101 ctcaaggccg agttcaacag cgaaccgcat gtgcgcggca ccgcctcgat ggcgcgcgcc
     5161 gccagcccgg attccgccga tagccagttc ttcatctgct tcgatgatgc ctccttcctc
     5221 aacaaccagt acacggtctg gggcaaggtg acggcgggca tggagaacgt cgacaagatc
     5281 aagcgcggcg agccggtgca gaatccggac aagatcgtca aggcacgcat ggccgccgac
     5341 gcggcgtgac gccaaaatcg tcatgcgcgc gcttgacccg cgtatccatc aatcttcaga
     5401 acagtctttc gtagaagatg gattgccgga tcaagtccgg caatgacagt ctggccatgc
     5461 gcaccgacct cttcgacttc gatcttccag caggccacgt cgcgctgcgc ccggcgagcc
     5521 cgcgcgattc cgcgcggatg ctgatcgtgc aacccgatgg tgtcttgctc gaccgcgtcg
     5581 ttgccgatct tccggattgg ctcgagcccg gcgatcagct ggtggtgaac gataccaagg
     5641 tgatcgcggc aaagctcagt ggccgccgca tcggccgcga caccgagccg aagatcgagg
     5701 cgacgctgat caagcgtctc gacggctcgc gctggcaggc gctggcgaaa ccggcgaaaa
     5761 aattgtcaga gggcgatgtg gttcgcttcg gcaatgaggg caaggtgtgc cttctcggcc
     5821 atctcgatgc ggaggtcgag cacaagggcg aggatggcga ggttacgctg tcgttttcat
     5881 ttcacgggcc catgctcgat caggcgatcg gcgatctggg cgcgccaccg ctgccgcctt
     5941 acatcgcttc aaggcgcgcg cccgatgagc gcgatgccag cgactaccag accatgttcg
     6001 cggtcaatga gggtgcggtc gcggcgccga cagcgggact gcattttacc caggcgctgg
     6061 agacagcgtt gcgcgaacgc ggtgtcgaaa ttcaccgcgt aacgttgcat gtcggggcag
     6121 ggacctttct gccggtcaag gcggaagata ccagtgcgca caagatgcac gccgaatggg
     6181 gcacgatctc ccgcaccacg gccgatgcgc tgaatgcggc gcgcgccaaa ggcggtcgcg
     6241 tcgtcgccgt cggcaccacc gcgctgcggc tgcttgaaag cgccgccgcg gaagatggcg
     6301 cgatcgagcc gtttaccggc gagaccgcga tcttcatcgc gccgggctat cgatttcgcg
     6361 cggtggacat tctcatgacc aattttcact tgccccggtc gacgctgttc atgctggtgt
     6421 cggctttcag cggcctcgac acgatgaaac gggcctatgc ccacgcgatc ggcaacggct
     6481 accggttcta ttcctatggc gacgcctgcc tgctgtttcg tgataagtcc acctgatgac
     6541 ctcagccaat cattttgaac tgctcggccg tgacggcaag gcgcgcatcg gccgcctgac
     6601 gacgccacat ggcgcggtac gaacgcctgc tttcatcccc attggtaccg gcggcgcgat
     6661 gaagggcttg cattggcgcg aggtgcgcga tgccgggacc gatatcgtgc tcggcaatac
     6721 ctatcacctg atgctgcggc caggcgccga gcgcatcgca gctctcggcg gcctgcaagc
     6781 ctttaccggc tggaacggac ccatgctgac cgactccggc ggcttccagg tcatgtcgct
     6841 tgccgaattg cgcaaagtgc gcgaaagcgc ggtcaccttt cgctcgcata tcgacggcgc
     6901 catggtcgag ctttcaccgg aacgctcgat cgaggtgcag cggctgctcg gctccgatat
     6961 cgccatgcag atggacgaat gcatgcggct gccggcggag cgcagcgata tcgagcgcgc
     7021 gatgcagctc tcgctgcgct gggcggagcg cagccggcgc gccttcgagg ccgcgcccga
     7081 tggatacatg gtgttcggca tcgcgcaagg cggcgacgtc ccggaactgc gtcaggccag
     7141 cgcgaggggc ctcctcgaga tcggctttca cgggtacgcg atcggagggc tcgcggtcgg
     7201 cgaaccgcaa gcggtgatgc tcgccatgat cgatgaggtg gcgccgatcc tgccgcagga
     7261 tcgcccgcgg tatctgatgg gtgtcggcac gcccgaagat attctggagg cggtcgcgcg
     7321 cggcatcgac atgttcgatt gcgtgctgcc gacgcgcaac ggccgtcacg ggatggcgtt
     7381 cacgcggttc ggccagatca acctgcgcaa cgcccgccac gccgacgatc cgcggccgct
     7441 cgacgaagag agttcgtggc catccacgcg cagttacgcg cgcgcctatc tgcatcacct
     7501 cgtcaggtcc ggggaaactc tgggcgcgat gctgctgtcg gaaatcaacg tcgcgtatta
     7561 ccagcgcctg atgcaggaca tcagggacgc gatttcgaag ggcatattcg aaagcttttg
     7621 cgaacgcacc cgcgccgaat gggcgagggg tgacatcccg ccgcgctaga tacggttgcc
     7681 aaaaggaagg cttagttgca ggcaaaccgg cggagcgaat gcttggtcac gaaaccgtaa
     7741 ccgcaggtgt cgcaggtcca gagatagctg acgacattgt cggaaacaaa agcggaagcc
     7801 tccgccgcca ccatggaatc ggcgcaaacc gggcaggtcg gcagatcgca tacgcgcggg
     7861 tcacccttgg gcgcgacggt cgacatgact tcagcaagtg ctggcatcat cacctcctgg
     7921 cgatctcaaa cttaagtcta gcatacaccg ggctgtttga cgatagcgca acacaaatgc
     7981 gttcgtttta cgttatttgg ctattttccg attttgcaat gcagcgaatg ttcaacgctc
     8041 ggcattctgc cttgcacacg ggcacaagct gcccctaatg aagaaagtcc ctaatttcgc
     8101 gatcaacttg cggccagagg agttgtcatg gacgcgtcgt cagctacgag tgcagtgcac
     8161 cgtcccggcc ggggccgtgt cttcgacagc atcgtcgatg caatcggcga tacgcccatc
     8221 gtccgtttgc gaaaaatgcc gcagcagcac ggcgccaatg cgacaattct cgccaagctg
     8281 gaatatttca gtccggccgc gagcgtgaag gaccgcatcg gggccgcgat ggtcatcgcg
     8341 atggaaaagg cgggcctgat caacgccgat accgtgctga tcgaaccgac ctcgggaaat
     8401 accgggatcg cgctggcgtt cgtcgcggcc tcgcgcggct accggctgaa gctggtgatg
     8461 ccggaatcga tgtcgatcga acggcgcaaa atgctggcgt tcctcggcgc tgaaatcatt
     8521 ctgacgccgg ccgctcaggg catgaagggc tcgattgcga ccgcggaaga actggtgcgg
     8581 accacgccca acgccgtcat gccgcagcag ttcaagaacc tcgccaatcc cgaaattcac
     8641 cgccgcacca cggcggagga aatctggaac gacaccggcg gcaacatcga tatcttcgtc
     8701 gcgggcgtcg gcaccggcgg caccatcacg ggcgtggggc aggtgctgaa gccgcgcaag
     8761 ccctcgctgc ggattgtcgc cgtcgaaccc gaggagagtc cggtactttc cggcgggcag
     8821 cattccccgc acaagatcca gggcatcggc gcagggttca ttccggatat cctggatcgt
     8881 tccgtcatcg acgaaatcgt caaggtcaac agcaccaccg cgatcgaaac ctcgcgggcg
     8941 ctcgcgcgca acgaaggcat tgccggcggc atttcctcag gcgccgcgat cgctgcggcg
     9001 cttgcgatcg gcaagcggcc ggaaaacgcg ggcaagacca ttcttgcggt cgtgccgtca
     9061 ttcgcggagc gctatctgtc gaccgcgctg ttcgaaggaa tctaatcgtg ccagatcaac
     9121 cgagacgacc aagaactttg aacgacgccc gaaccgaagc cgaagctgcg ttcaagcgga
     9181 ccacaaccaa ggtgccggag gcgccgccca aaacggccgc gcttccgagt gtgaaggaac
     9241 tggtttcgct gcggatcgac caggatgttc tcgaacactt ccaggagggc ggacccggct
     9301 ggcaggatcg catcaatgat gcgctgagga aggcggcagg gaaataggct gggcagggtt
     9361 catgcgctca catctggcgc gcatttggtt tgcgcttctt gccggtgcgc agatgacgcc
     9421 ggcgtcggcg gaatcggtgc cgttcaactg cgtcattttg ccgggcagtg agctggccag
     9481 catcatgatg acaaattcgt tgccgggcga cgcctcctgc atggtgacgt gcaagttctc
     9541 aacgaccaaa tacgacaaca atccacagat cacctgcgcc aaaccgatac cggccggcaa
     9601 ggaagtggaa atgtgccggc tgtcgtccgg cggcgacaaa atggtgaaac tgatggaggg
     9661 gcatgcggag tgcacgagat aactcgacgc ggaaccaaga tcccggaatt aaaaaggccc
     9721 cggatatgca gcgctgcgcc aacccaattt cgatctggga gtgtccacgc gatcggtgct
     9781 aagggaacct cctgctgctg tcatttccgg gtctggccct gcggaccatc gcggaatgac
     9841 gccaccagca aacctggaga gactgctcat gcgtatcctg caaccggccg actggccaaa
     9901 gccgcgcggc ttttcgcacg gcgtcacgct cgatggtccg ggccaatgga tcgtgctcgc
     9961 cggccagacc ggtggcgacg agaagggtga ctatcaaccc gatatggcca accaggtggg
    10021 gacggcgctg cgcaggatcg tcaagctgct tgaagaggct ggcgcgggtc cggagcacat
    10081 cgtccgtctc acctggtatt tgaccagccg cagcgagtac gaagccgcgg gcgccgctat
    10141 cggtgcggcc tggcgagaga cgctgggacg aaactttccg ccctcgacgc tgctctacat
    10201 ctcaggcctg gtcgacgagc gcgcgaaagt cgagatcgaa gtcactgcgt tcggaccggc
    10261 cggataggac ctcccgatga ccaagcgatc cacatcggcg gattttgtga ccgccttcgc
    10321 caccggttgg ccggagaacc agccggaaat catggtgctg tcgctgacca cgcaaaaagg
    10381 cgtccaggac tttgccctca gcaaggaaca ggcgctgctg atcgccaaga ccatgaaaca
    10441 aaccgccgcg caattggcga agcctaagca agcttagctg agagcaggcc gcggcgaacc
    10501 aactaatggc gggtcagtgc gatgttggcg cccctgaact ggctcgcagc cgtgatcacg
    10561 acggactgcc ggttgccgcg cgtcgccagg gaaaggtttg ccgtgaaacc gggcgcgttg
    10621 gcgacaacct gaaaattgcc gttgcttccg cgtccctcga gggtgccgtt gatgccgcgg
    10681 ctgctctcgc tccacgtccc ggagagcgca ttgccctgcg cgaccacatt gcttgagaga
    10741 atgaacctgt aactgtcgct ggcgcaggtg aggctctggt tgaggccggt cccgcccgcg
    10801 ccgactgcgt aggttgcgcg gcagcgaatt cgctcgctcg atccgtcgtc gagggtgacg
    10861 gtgcccccgc ccgaccagtt gccggccatg cccgcgaacg gccccgattg cgcatagctt
    10921 gcggagccgg acgccgatgc cacgaacaac gcggtgacga tgaatatcag ccgcgtgaca
    10981 cccgcgaagc tgccagtcgt caggcctgtt ttattgccgg gatgcttccc accgaccgct
    11041 gcacggtacg cctgcagatg ccccattcca tttccccgat ccggagttgc cgttgagttg
    11101 gccatttgca tatgcaccag cgatggagac tcgcacaagc ccctcacggc cgatgctgcc
    11161 ggaaacgctt gcgccgggag cagaaacttt gccgtcggaa acggtaagtg ttgagcgcgt
    11221 cgtcggctcg caactacccg ccttggtcac gatcgtgaca ttccaaagcc cgtcataggg
    11281 cgtctgggca accgcgggag cagccgcgag gacggtgagg
//
LOCUS       DATNOI010000675        21646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1691819,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000675 DATNOI010000000
VERSION     DATNOI010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 21646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21646
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691819"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VN968_27035"
     CDS             complement(<1..186)
                     /locus_tag="VN968_27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C13, legumain asparaginyl peptidase"
                     /protein_id="HXL32920.1"
                     /translation="MTSNSWIGRFGAPFIAFVLTVVPSASPAHAVEDARKVSVVSFGL
                     FGDQGVFKSEATGAAQVV"
     gene            734..1375
                     /locus_tag="VN968_27040"
     CDS             734..1375
                     /locus_tag="VN968_27040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HXL32921.1"
                     /translation="MDECCAPPPLNPGASKKQDRAHRRVLWAVLAINAVMFAVEVIAG
                     VAAGSAALQADALDFLGDAANYSISLMVVGMALRYRASAAMAKGATMAAFGLWVLGTA
                     VWHSVHTTLPGAFTMGTVGVAALVANAASFGLLWAYRHSDANMRSAWICTRNDVLGNL
                     AVLIAALGVFGTGTGWPDIIVAAIMAALAIQGAALVIRQATAEWRVGKLAAAE"
     gene            1590..2627
                     /locus_tag="VN968_27045"
     CDS             1590..2627
                     /locus_tag="VN968_27045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32922.1"
                     /translation="MIGRAFIPAYGVCENTAVEADSNTLPILGFMGSVVEIWNPIVSD
                     IGVNFPFWFIAAYFVVVGWYVSVPAIVVLGLVGCFVDMNRGWRVAAFGTAALLSAPFV
                     VYAVLTIIDNAKYKRFQAEVHRTLDHNETVTGLALPAGSDIYFTDKSQTQILSVRLPV
                     VTDIRGVRVTEMLMWQDLNHVWQSTLAGDQIVAGLPCAAGFITFDNDGAVYECRLAAA
                     HPFLGFTLPPGTTLTRGADQAHWNLHLPDNAGLVIPALAATAPPGATLHVTHNGRLES
                     ASSGNGATMVVRGVPLDSMNLLVRGDRVVAPLKEPFAVAGEMRPADTAVSIDLTTGDV
                     ALAGKNWWLSP"
     gene            complement(3232..3873)
                     /locus_tag="VN968_27050"
     CDS             complement(3232..3873)
                     /locus_tag="VN968_27050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXL32923.1"
                     /translation="MSPFFSLCGSGTSRSFALRCAGSSLWTPNKLGKAVETEQRRAAA
                     CAWLATGPCRLNPQFFGEGCLAGQSLRTTTMSALGNCQVTSITRAAMIAALNNQPRFS
                     RLFMDHLLRRNSRIQEDVINQLFNSSERRLARLLLLLANYGKEGGPPIVPVILGQETL
                     ADMIGTTRSRVSFFMNKFRKLGLIDYKQEIDVHPSLLNAVLHDKPEIGADEPA"
     gene            complement(3870..5381)
                     /locus_tag="VN968_27055"
     CDS             complement(3870..5381)
                     /locus_tag="VN968_27055"
                     /inference="COORDINATES: protein motif:HMM:NF009120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXL32924.1"
                     /translation="MSRFVNPKRKQLGLWPVRLAMAGLAALIAAGDHAGATDRRSERF
                     VESIEQRSAGEPIMAIVSLHSQRITVYDANGWILRAPVSSGQQGRETPAGVFSVIQKE
                     AEHYSNLYDDAYMPDMQRITWSGIALHGGRLPGYPASHGCVRMPFDFAERLFDATRLG
                     LRVIVAPTDVVPVEIAHPALFPSKPGAGALAAARAAEADEAARKADQARLAAGTAFRE
                     AAQATAPVRVAENLKHRAEAQLAAAETVLGSAIPAEARAQAERDKAQAVDRIAALQAQ
                     LDGAKAQLQPKLDAVTRTREAAVIAETARAAAAEAARQVARQSEPVSVLISRKTQRLY
                     VRQAFEPILESPVTILDADRPIGTHVFTAMERTNGDTDMRWSVVSLDGGRPHDGVVEL
                     RDQARGGHGRDAEPMPTDPGSAKTALDRIVIPPDALDRIAEFAPRSSLIITDEPLSSE
                     TGKGTDFVVLLSGEPQGGIKGRRRGPGTEFGYARPRDRLPFWRSPFGGPFSSW"
     gene            5611..6093
                     /locus_tag="VN968_27060"
     CDS             5611..6093
                     /locus_tag="VN968_27060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32925.1"
                     /translation="MSYLGFEQGKALDKVIIQSKSAGSVPISERSVAALLNIWMAWGD
                     FTLSGMQNGFLRLVNSVPICSPCTMPMRYAAQAVCAWLPVAPAQSPRSCSTVIVSHDP
                     EPIAQPRNAIESSNLRILSPRFCVGYNGRSYGSQRRPDPSHAARQRASVEEISLRELS
                     "
     gene            complement(6719..8656)
                     /locus_tag="VN968_27065"
     CDS             complement(6719..8656)
                     /locus_tag="VN968_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXL32926.1"
                     /translation="MAQGMKRLGMPIAALIGVALIGLISTSWFLDRDALRQAVEAQIR
                     AVTGLDLVIKGNIDVSVFPGSYVSFHDVGLKGGGTADPALAVDVLTANLRLLPLLLRR
                     FEIADVMMLRPHIHVTRDAGGGSNWTPFIDTIARAMKPGADNQVSFSEIRIQDGALSY
                     EDAASHLSEKLGDIDLSLAWPSISRSFAATGQFDWRGERVDGSISASDFVAALSGDRS
                     GLKARLASAPLKLAFDGTVANRTSTMMEGTLTVDSISLRNALRWAGQAPPGSGGFGRF
                     ALRARANVVGASIALTNVNIELDGNVAEGVLTYVNNGRQTLQATLAAGALDFTPYVST
                     FRLLASGAHDWNRQLFDLNSLSTTDLDMRLSAARVTVNSTKLGRTAFGANLRGGALAL
                     SVGEAQVYGGIAKGSFGIARSDAVADVKAQFSFIDVDLQSCASELFGVSKLSGRGNLN
                     VSLVASGSSPFGLAQSLDGTASLTAHDGAISGFNVEQLLKRLERRPLSGSGNFRTGST
                     PYDNLNIALKFSEGIATAEDVRVEGPAARITLSGTASVPAREYDMKGIASLTSVPNAP
                     PGFDLPFVVQGPWDDPLIFPDPESLIRRSPASAPLLDAVKDRKTRDAVRSVLERFIGG
                     GEKPAAPDAAAAPAETAAKPN"
     gene            8971..9678
                     /locus_tag="VN968_27070"
     CDS             8971..9678
                     /locus_tag="VN968_27070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HXL32927.1"
                     /translation="MNKVYPDAKSALDGILRDGMMIMSGGFGLCGIAETLSDALRDSG
                     VKNLTVVSNNAGVDGIGLSRLLETRQIRKMISSYVGENKLFAQQFLAGELELEFNPQG
                     TLAERIRAGGAGIPAFYTRTGVGTLIAEGKEVKEFDGEKYIMERGLFADLAIVHAWKG
                     DTAGNLIYRKTARNFNPMMATAAKVTVAEVEHLVPAGELNPDHIHTPGIFVKRIIEVG
                     TGNKRIEFRNTRPRPAA"
     gene            9701..10351
                     /locus_tag="VN968_27075"
     CDS             9701..10351
                     /locus_tag="VN968_27075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HXL32928.1"
                     /translation="MAWTREQMAARAAKELRDGYYVNLGIGIPTLVSNYIPDGIDVNL
                     QSENGMLGMGPFPYEGEEDADLINAGKQTVSELPTTSYFSSADSFGMVRGGHIDLSIL
                     GAMQVAQNGDLANWMILGKMVKGMGGAMDLVAGVKRVVVVMEHSAKDGPKLLKKCNLP
                     LTGEKVVDLVVTDLAVFTIDKHGKDGMALIELADGVTLDEVKSKTEAEFRVALKNA"
     gene            complement(10413..10841)
                     /locus_tag="VN968_27080"
     CDS             complement(10413..10841)
                     /locus_tag="VN968_27080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HXL32929.1"
                     /translation="MGYLWLKAFHVAAVIGWIGGMLVSSLFIAMHVASSRPLAAGEAR
                     MIEIVRAWDWKVTTPAMLLAWALGMTMAVQAGWFSSRWLIIKLVLVFGLSALHGVQSG
                     ALRRMARGANRWTPAHLCYAAPAILVAIAAIAALAVVKPF"
     gene            complement(10823..11194)
                     /locus_tag="VN968_27085"
     CDS             complement(10823..11194)
                     /locus_tag="VN968_27085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HXL32930.1"
                     /translation="MPPTETTARTDERNQLRRMRLISLLEGATLVALLFVAVPLKHIA
                     GHPAATSIIGPIHGMAFLLYVWMLIQTVSGADWSRAEIARAVIVAFIPFGAFANERFL
                     KRKELALAGSVSPERKWDTSG"
     gene            complement(11194..11457)
                     /locus_tag="VN968_27090"
     CDS             complement(11194..11457)
                     /locus_tag="VN968_27090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32931.1"
                     /translation="MAEHANTFLLLTILVLVTILLVFGMKYFSAARTVHARANGEEAY
                     RELAEKAVALQAANAASQAAIQADFSEMKIRLTAIEKLLREVE"
     gene            complement(11471..11770)
                     /locus_tag="VN968_27095"
     CDS             complement(11471..11770)
                     /locus_tag="VN968_27095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXL32932.1"
                     /translation="MRALQALANPHRMRIVAALRSRGRNYVSQLARDVGISRPLLHLH
                     LQKLEEAGLVTSKLELSGDGKALNFFEATDFSFELSPTAIAAAVKSLSIQSSKSD"
     gene            complement(11984..13759)
                     /locus_tag="VN968_27100"
     CDS             complement(11984..13759)
                     /locus_tag="VN968_27100"
                     /inference="COORDINATES: protein motif:HMM:NF040606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme-cytochrome C peroxidase"
                     /protein_id="HXL32933.1"
                     /translation="MKAFSKVLLFSLAAMVSTIDPTFAQPASGALAAPTLQYAKQGWT
                     AEDRQAFYTTSQGSHMIPYPWFKALRRLDVDEPFGGDQLARYGYLPNDTKLNPEGLPV
                     GFVIDGDAKTGFLGMTCAACHTAQIEYQKDGATQQLRIDGAPATANFQLFLTDLEAAA
                     KETIKDTNRLEKFARSVLGTNSSAAKVATLKTDFGAWVKQYSAFMDASLPASSPWGPG
                     RLDAFGMIFNRVAARDLGIDANFKVADAPVSYPFLWNASRQDKTQWNGFVPNGLFIHA
                     LGRNTGEVFGVFADFKPSKRAVPPPHDFSNNSVKFAGLQQLEEKIAVLKPPPWPFELK
                     QELVDKGRVLYAQNCGSCHEEQPLLDGTWLTPVKAVGTDPRMFKNADRSSQSGLLAGA
                     LTPNPPGATLAETAKTHDILATTVVGALINEAFPLTFRNRNNGVWQAIRKDLSHLLPE
                     SQNTPIVVLLLRPSVRANLESHVRDRLKTMFVPLAPDAPPAYESRVLHGIWATAPYLH
                     NGSVPNLWELLLPPERRKPSFMVGSKVFDPVNVGYVTDSSPFNDGKLVVGPGAEPGNS
                     NAGHDFGKDLSDDERWALIEYLKQL"
     gene            complement(14005..14850)
                     /locus_tag="VN968_27105"
     CDS             complement(14005..14850)
                     /locus_tag="VN968_27105"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL32934.1"
                     /translation="MIIPAFGWRPMFIIAGVCSLIVWYLRKNLPESPRWLESQGRIAE
                     AEALMQAIEKESTGGGTLPPVVVPAPVPQVSASDMLRPPLLQRMLVGCWVLITINTLI
                     FGFVIFLPQFFLRQGLTIANSLAYTLVLSVASLVGCAVGAYLSDAIGRRASIIGSSIV
                     TIVAGYIYARFSAASDPAIVLSVGFVLIVAIYVQTAILFGVYTPELFPTEIRLRANGI
                     CNTFGRGATVVSPFIVGALMASYQLPGVVWLMIGLVVVQIVVVWAWGVEPRNRGLEDV
                     ATVTA"
     gene            complement(14741..15352)
                     /locus_tag="VN968_27110"
     CDS             complement(14741..15352)
                     /locus_tag="VN968_27110"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL32935.1"
                     /translation="MAQAVNAGARLDRLPISSFHYRVFWLVGAGMFFDGYDLYIAGGV
                     LASAVQTKFSTVPQNLQFISLTFVGMTLGALITGFVGDKFGRRFTYQINLLIFGLASL
                     AAAFAQDMNQLIACRFVQGLGLGAEIVVGYSTLTEFVPPKTRGRWLSMMAFLVVAGFP
                     GDRFARLSDHPGLWLAPDVHHCRRLLADRLVSAQESSGVAALA"
     gene            complement(15435..16964)
                     /locus_tag="VN968_27115"
     CDS             complement(15435..16964)
                     /locus_tag="VN968_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HXL32936.1"
                     /translation="MNISAKPTAVDVEIHGNFIDGREIEAGSGQMLDVRNPATGDVIA
                     RIPNSTADDIDRAMKSARAAFEGRTWGGMDIRARARLVNRLADAFEANLESLYRLETL
                     NNGRPLNETRAQLSRLPDFFRYFAGLALARRDAVIPVEGSYLNYTLRTPIGIVANCTP
                     FNHPLMIMCKSLAAVLASGCVTVVKPSEYTPLTTLKLAQIFTEAGLPPGVFNVVLGLG
                     HSAGKMLAEHRDINKLVLTGGTEAGRIAGAAAAKVFAHQTMELGGKTPVMVFDDYDID
                     QAVNYAAFGAFIGAGQTCVCASRHIVQASIYDEFVERLKAKTKAIRIGDPFDPATQLG
                     PVISARQRDRVLSYSQYGRDDGARLVTGGVAAKVPGHEGGFFVEPTVFADVKPDMRIF
                     QEEVFGPFTSVTPFKDEADALRLANDSPFGLAAAIRTRDISRAHRVASAVKAGIVWIN
                     DHHRLDPASPWGGVDDSGLGREFGTESFDDHFNTKSVMVATHDQPFDWYRDTATQRRL
                     N"
     gene            complement(17081..17716)
                     /locus_tag="VN968_27120"
     CDS             complement(17081..17716)
                     /locus_tag="VN968_27120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXL32937.1"
                     /translation="MLLRDNVYENLRSDILTCRLAPGEDMREQELAERYAVSRQPVRE
                     ALLRLEREHLVTVQPRQGYRVNPISLSDARDLLRFRLALEPACVAEAIENATAEVLTA
                     LDEFRAFAGDHEDFIAYNRAFHSALAHASGNRRMAAALCDLIGQADRLVRVSVANVKG
                     HDPTQLVAEHVALIEAMQRRDARGAVRLIKAHVLQTERRVLPALKRNAVIV"
     gene            17874..18653
                     /locus_tag="VN968_27125"
     CDS             17874..18653
                     /locus_tag="VN968_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL32938.1"
                     /translation="MNRLFANGTVHAMQSGKGPFLFLLHSLLSDRASFDAVVPRLSQS
                     FRVVVPELPGFGGSEAVSGGLAAVADRMAEALTDAAGGDDVFVLGNGYGGFVALQMAI
                     RHPDIATKLILADCGAAFSEEGRQAFRNMAAASQARGLSVITDVAMRRLFAPEFQAGH
                     PDLMHDRREAFLRTDPEVFHAACDALAALDLRPELSKVEVPVLVLVGEHDEATPPQMS
                     RELAAGLPQANLKIIPGCAHVPQLQSPEVFLEAIGDFLPVA"
     gene            18809..19294
                     /locus_tag="VN968_27130"
     CDS             18809..19294
                     /locus_tag="VN968_27130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXL32939.1"
                     /translation="MHGVLDYCTGRIERSLAAGATVIREGETTDHLYVLIEGRLEVVK
                     GETVVAIITEPGAVLGEMSVLLDQPHTATVRAASDSTVYEISDAASFLREQPAVALLV
                     ARLLAQRLNVATTYLADIMQQYAGHGDHLAMVGEILQSMINLPPIKISPGSDRQSDPR
                     M"
     gene            complement(19248..20060)
                     /locus_tag="VN968_27135"
     CDS             complement(19248..20060)
                     /locus_tag="VN968_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXL32940.1"
                     /translation="MRCLIVADLHYSLPQFDWLLSAAPQFDLVIFAGDALDIGSAVDF
                     RAQIVVVKKYLARLAGLTRVILCSGNHDLDERNPEGEKIARWIGDVRALGIACDGDSL
                     TIGDTLFTVCPWWDGPLVKQRIDDQLREAAAARLQRWIWVHHAPPANSPTSWGGKRFF
                     GDVELVQWIQRYQPSMVISGHVHQSPFIPDGSWFDRLGGTWVFNAGLQAGRPPVHIVL
                     DIDSNTAFWLPLGDEQYIDLNAPLQRPAAPVSHPPAWLTSLGRIADPNLVRS"
     gene            complement(20259..>21646)
                     /locus_tag="VN968_27140"
     CDS             complement(20259..>21646)
                     /locus_tag="VN968_27140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HXL32941.1"
                     /translation="ALEDAGGLDALYVEPGVSSASSTSDTGRPVTISGTEASSEGAPA
                     IDARLFTISWDGDSALALIFSGTRNEAAAAVAVLPEFIPFRAPSAVSQANAEELGAIL
                     DATAEGIVMFDADGHINFCNRGAEALFGYDGTEFAQRNLIELFAPESQPAVLDHLNGI
                     KGADAASLLDHGRDVLGRVRKGGIIPLSMTLGRTRPDGPNFFAVFRDVAQSGKSEREL
                     LQAGQLADRAANAKADMLARISHEVRTPLNAIIGFAEVMISERFGRLANKRYIEYIKD
                     IRISGERVIAVINDLLDLSRIETGRLDLTFASQDLNDLVEQCVAVMQPQANRERIIIR
                     TSLAQMLPPVIADAHALRQIALNLIGNSIHLANAGGQVIVSTALSDFDEVVLRVRDSG
                     QGLNDNEVAAALEPFRTQAPSDQASDGSGVNLSLTKALVEANRAHFHIKTASHSGTLI
                     EVTFSQARAKA"
BASE COUNT         4093 a         6646 c         6775 g         4132 t
ORIGIN      
        1 tacgacctga gctgcgccgg tcgcctcact tttaaacacg ccttgatcgc cgaaaaggcc
       61 gaaggacact acgctcacct tgcgagcatc ctcaacggcg tgcgccggcg aagccgacgg
      121 cacgacggtc aaaacaaaag cgatgaacgg cgcgccgaac cggccgatcc aggaattgga
      181 agtcatggcg aacctcgtat cgcgaccgcc accgtatcat agcaacgcag acggaattgc
      241 ggaattacgg tgaatctcca ttcctcggga cggcacttct cacgatgccg caaacaaacc
      301 ttggaggact tcaaagggtt ggtgcatgcc catgcgtcgc cgacgatcca gtcgtctcca
      361 ggctccttgc tgcggcatct cacgaccctg atgtattccg tgcgatgctc gagaccgtgc
      421 tgtgcgttgc actgccccaa gaagtgatgg cgaggccgta cgtggccgca aagatggccg
      481 aattcgaggg acatgcattg ccgccggacc atcacatgga tcgaaatcgg ctgctggcct
      541 tgctggataa tagatagatt atttcgccgg catcccctgg ggttgctgct ggtcatcagg
      601 caagttgacc cggtgccgtt tggtatcaat tttggtacgc gtcttgtgtc cggcatccga
      661 aaatcttcac aacaggtgac atggcgtgtc ggcgcaacct ggcgcgcgcc ggcaaacgac
      721 ttatcttgtc cccatggatg aatgttgcgc cccgccgccg ttaaatccgg gtgcctccaa
      781 aaagcaggac cgcgcccatc gccgtgtgct ttgggcagtg ctggcgatca atgccgtgat
      841 gttcgcagtg gaagtcatcg caggagtggc ggcaggatcg gcggcgctcc aggccgacgc
      901 gctcgacttt ttgggtgacg ctgccaacta ctcaatcagc ttgatggttg tcggtatggc
      961 gcttcgctat cgagcatcgg ctgcgatggc caagggcgcc acaatggctg ccttcggtct
     1021 atgggttctc ggaacagcgg tctggcactc cgtgcacact acactgccag gcgcattcac
     1081 catgggtacg gtaggcgtcg ccgccctggt tgcgaacgcc gcgtcattcg ggctgctttg
     1141 ggcctatcgc cacagcgacg ctaacatgcg ctccgcatgg atttgcactc gcaacgacgt
     1201 cctcggcaat ctagcggttc tgatcgcggc gcttggcgtg tttggcactg gaacgggttg
     1261 gcctgacatc attgttgcag cgatcatggc tgcactggct atccagggcg cggctctcgt
     1321 gatccggcag gccactgccg aatggcgggt cggtaagctg gccgctgccg agtaacaatt
     1381 ggacgctcta actgccttcg gcctattcgc cgtgacagcc atgttggttt gctacgcgct
     1441 ggaagatcgc agcccgtggt tcgtgccggc gttcgccgga tcacgcgcgc tcggttcggc
     1501 ccatggattc ctgcaaggct cgtggccgtt ccgggttgtg agggcgattg ggcgttgatc
     1561 gctttgcgac ctgttgctca gagagcgcct tgatcgggcg tgctttcatc ccggcatatg
     1621 gtgtttgcga aaacaccgcg gtagaagctg acagcaatac gctgccgatc cttggtttca
     1681 tgggcagtgt tgtcgagatt tggaatccaa ttgtgagcga tatcggagtc aatttcccct
     1741 tctggttcat cgccgcctac ttcgtcgtcg tggggtggta tgtgagcgta cccgcgattg
     1801 ttgtcctggg tctcgttggc tgttttgtcg atatgaacag gggatggcgc gtggcggctt
     1861 tcggcaccgc cgcgctgttg tcagcgccgt ttgtggttta cgcggtcctc acgatcattg
     1921 acaatgcgaa gtacaaaaga tttcaggctg aggtgcatcg caccctcgac cacaacgaaa
     1981 ccgtcaccgg cctggcactg cccgccggca gcgatattta cttcaccgac aaatcccaga
     2041 ctcaaattct ctcggtccgt ctgcctgtgg tcaccgatat ccgcggcgtg cgggtgactg
     2101 agatgttgat gtggcaggat ctcaatcacg tttggcagag cacacttgcc ggcgatcaga
     2161 tcgtcgctgg ccttccgtgc gcggccggct tcatcacctt cgataatgat ggcgccgtgt
     2221 atgagtgcag gctcgcggca gcgcatccat ttttgggctt cacgctgccg cccggcacca
     2281 cgctcacccg aggcgccgac caggcgcatt ggaatttgca tctgcctgac aacgccgggc
     2341 tcgtgattcc ggcgctcgca gcgaccgcgc ctccgggcgc cacgcttcat gtaacgcaca
     2401 acggacggct cgaaagcgcc tcctccggca acggcgcgac catggtggtc cgcggcgttc
     2461 ctctcgacag catgaacctc cttgttcgcg gcgatcgcgt cgtcgcgccc ttgaaggaac
     2521 ccttcgcggt ggcgggcgaa atgaggccgg cggatacagc cgtgagcatc gacctcacga
     2581 ccggtgatgt cgcgctcgcg ggcaagaact ggtggctatc gccatgatgc gggcaaagca
     2641 gaactcatgc cattcagatg tctcttcgcg accgggctga acgcatgagc gccgggcccc
     2701 cggcccgtcg cgcgggcggc ctcggatcag cctgccctgg ttgttcgggt tggggtacgt
     2761 ttaaggcagc catgaaccag tcgcgcgcct tgcgatatcg gaaactcgcg cggcgtcgcc
     2821 gataggcatg atcgcggtca gcttgccgca cgctcaaaaa aggctggtcg agagcagtat
     2881 tgcactgaca gtcccggcaa tgaggcgtta aatcccgatt gtcgggaagc attgccaaat
     2941 gctctcgacg tccttcgaaa cattgaaaaa cccccggttt caggcccggg ggtttttttc
     3001 gtcacgcggg gttacatccg ccctcacgca acgataaccc atattgtcac gttattcgcg
     3061 gctgtcaacg aagtgcccgc tgcaaccggc gaatctggaa gccaaagttc ggcatgttgc
     3121 ggtccttgtg aacccacaac aattttaacc ccgagaatag ccggctactc ccgccgaaaa
     3181 gcaggacggg actatcccgt tggcggaatg ccaatcggcc agtacctgca gctatgcggg
     3241 ctcgtccgcc ccgatctcgg gcttgtcgtg cagcacagca ttcaacaacg atgggtgaac
     3301 atcgatttcc tgtttgtagt cgatcaaacc cagcttgcgg aatttgttca taaagaagct
     3361 cacgcgggat cgagtggtgc cgatcatatc ggcgagtgtt tcctgaccga ggatcacagg
     3421 gacgatgggc gggccacctt cttttccgta attggcaaga agtaaaagaa ggcgcgcaag
     3481 cctcctctcg ctggaattaa aaagctggtt gattacgtct tcttgaatcc tgctgtttct
     3541 gcgcaacaga tggtccataa agagtcggga aaacctcggc tgattgttaa gcgcggcgat
     3601 catcgccgct ctggttatcg aagttacctg gcagttgccg agcgcactca tcgtggtggt
     3661 tcgcaaggat tggccggcaa gacacccttc gccgaaaaac tgaggattca gtctgcatgg
     3721 tcctgtcgcc agccaagcac atgcggcagc gcggcgctgt tcggtttcga ccgctttccc
     3781 aagtttattt ggcgtccaca gcgacgaacc ggcgcatcgc agcgcaaacg aacgcgatgt
     3841 gccactaccg caaagtgaaa agaacgggct caccagctgg aaaacggacc cccgaaaggc
     3901 gagcgccaaa aaggcagacg atcgcgtggc cgcgcgtaac cgaattcggt ccccggaccg
     3961 cgtcgtcggc ccttgatgcc gccttgcggc tcgccgctca agagcaccac gaaatccgtg
     4021 cctttgccgg tctcggagct caacggctcg tccgtgatga tcagggaaga tcgcggcgca
     4081 aactctgcga tacgatccaa tgcgtccggt ggaatgacaa tccggtcgag cgcggtcttt
     4141 gcactgcccg ggtctgtcgg catcggttcg gcgtcgcgac catggccgcc gcgcgcctgg
     4201 tctcgtagct cgacaacgcc gtcgtgcgga cgtccgccgt ccagtgagac gacgctccat
     4261 cgcatgtcgg tgtcgccgtt ggtccgttcc atggcggtga agacgtgcgt gccaatcggc
     4321 cgatccgcat ccaggatcgt gaccggactc tccaggatgg gctcaaaggc ttgccggaca
     4381 taaagccgct gggtcttgcg gctgatcaac accgacaccg gctcggactg gcgcgccacc
     4441 tggcgcgccg cctcggctgc cgcggcccgc gccgtctccg cgatgacggc ggcttcacgc
     4501 gtgcgcgtga cagcatcgag cttcggttgc agctgtgcct ttgcgccgtc caattgcgct
     4561 tgcaacgccg cgatccggtc gaccgcctgc gccttgtcgc gctcggcctg cgcccttgcc
     4621 tcggccggga tggcggagcc cagcacagtc tcggcggccg ccaattgcgc ttcggccctg
     4681 tgcttcagat tttccgccac acggaccggc gccgtggcct gtgcggcctc cctgaacgcc
     4741 gtcccggcgg cgagtcttgc ctgatccgcc ttcctggccg cctcgtctgc ctccgcggca
     4801 cgggcggctg ccaaggcacc ggcacccggc ttcgatggaa acagggctgg atgggcgatc
     4861 tcaacgggca ccacatcggt tggtgccacg atcacccgca agcccagacg ggtcgcgtcg
     4921 aacaggcgtt ctgcgaagtc gaagggcatc cggacgcagc cgtgggacgc cggatatccc
     4981 ggcaggcggc cgccgtggag cgcgatgccc gaccaggtga tgcgttgcat gtcgggcatg
     5041 taggcatcgt catacaggtt cgagtagtgc tccgcttcct tttggatgac gctgaagacc
     5101 ccggcgggcg tctcgcgtcc ctgctggccg ctcgacaccg gcgcccggag gatccagcca
     5161 ttggcgtcat agacggtgat ccgctggctg tggagcgaaa cgatcgccat gattggctcg
     5221 ccggcgctgc gctgctcgat ggactcgacg aagcgttcgc tgcgacggtc tgtggcgccc
     5281 gcatgatctc cggctgcgat cagcgcggcc agtcccgcca tggcaagccg cacgggccac
     5341 agccctaact gcttgcgctt cgggttcaca aaacgagaca tcctactacg cgtcccatcc
     5401 gatcggggcc aagccggctt gcactcacca gcttgcaccc gggagactca taacgccatt
     5461 aggtcgaacc tgccaattcg gaacttgccg aatgcgcatg atcagccgga gtgtgccatc
     5521 cgatccggtc ggtcctcgcc tcgtccggca tcggcgtccg gccggtgcgc gttgttcgga
     5581 gcaccgacgc ctccaacaag gccgctggtc gtgtcctatc ttggttttga gcaaggaaag
     5641 gcactcgaca aggtcattat ccaaagcaag tcggcaggtt ccgtccccat ttcagaacgt
     5701 tcggttgcag ccttgttgaa catctggatg gcttggggag attttacgct gtcgggaatg
     5761 cagaatggct ttctacgctt ggtaaacagc gttccgattt gtagcccgtg taccatgccc
     5821 atgagatatg ccgcacaggc agtttgtgcc tggctcccgg tcgcgccggc acaatccccg
     5881 cgaagttgct cgacggttat cgtttcgcac gatcccgagc cgattgcgca gccaaggaac
     5941 gccatagaga gcagtaatct ccgcatttta tctcctcgtt tctgcgtcgg ctacaacggc
     6001 cgctcttatg gcagccaacg gcggcccgat ccctcccatg cagcacggca acgtgcgagc
     6061 gtcgaggaga tctctttaag agaactctct tgaatttggc aatcggcgac tccagctaat
     6121 caccgccaac cacctgacaa gttgggccgc cttgggcgtc gaacagtcct ggcgacgaca
     6181 tcgtcctttc cgttgtgaac gcttgtaacc aaggccaatt gcatgcaagg cgcccgatgc
     6241 tggacgccaa ctctagcatc tcatttttga cgacaggatg gtcggcttgt tcggcaagtt
     6301 tcagatactt caccgccttg tctcgcggat tgtggctgcc cccacgggct ggaaaccgac
     6361 cgattcgggg cgtggtgacg atgccagcga ttccggatca tctcgcggaa taaaaatccg
     6421 cgccggcgga tctagtagtg caaatagggt gtgaccggga aagcctcggt ggatctggcc
     6481 gcgccccgcg attgagagat cggccgcgac cccggcgcgg ccgttatgcg gtctgttttt
     6541 cctccaagat tgccggaccg cagggaggag cccgcggcgc tttaaaaaac gccgcgggct
     6601 tttttgtctt ccgccatgtg cgtggcggcg accgaagggc tgaaacactt cgaaccacgg
     6661 gtcagagtta ccaggcccgc ctgaccggtg caggttgcgg tgacgcgccg tcgaagcgtc
     6721 agttcggctt cgctgccgtc tcggccggcg ccgcggcggc gtcgggggcc gcgggtttct
     6781 cgccgccgcc gatgaagcgc tccagcaccg agcgaaccgc gtcgcgggtc ttgcgatcct
     6841 tgaccgcgtc gagcaagggt gcggaggccg gcgagcgccg gatcaggctt tccggatccg
     6901 gaaaaatcaa gggatcgtcc cacggaccct gcaccacgaa cggcagatcg aagccgggcg
     6961 gcgcgttcgg aaccgaggtc aggctggcga tccccttcat atcgtattcg cgcgccggca
     7021 ccgacgcggt gccgctcaag gtgatccgcg ccgccggtcc ctcgacgcgg acatcctcgg
     7081 cggtggcgat gccctcgctg aatttcaacg cgatgttcag attgtcatag ggcgtcgagc
     7141 cggtgcgaaa gttgccgctg ccggacagcg gccggcgctc cagccgcttc aacaattgct
     7201 cgacgttgaa cccggagatc gcgccgtcat gcgcggtcag cgaggcggtg ccatcgagcg
     7261 actgcgcgag gccaaacggg cttgagcccg acgccaccag cgagacgttg aggttgccgc
     7321 ggccggacaa tttgctgacg ccgaacaatt cggatgcgca ggattgcaga tcgacgtcga
     7381 tgaacgagaa ctgtgccttg acgtcggcga ccgcgtcgga acgcgcgatc ccgaacgagc
     7441 ccttggcgat gccgccatac acctgcgctt cgccgacgct gagcgccagc gcgccgccgc
     7501 gcagattggc gccgaacgcg gtgcggccca gcttggtcga attgaccgtc accctcgccg
     7561 ccgacagacg catgtcgaga tcggtggtgg acagcgagtt cagatcgaac aattgccggt
     7621 tccagtcatg cgcgccgctg gcgagcaggc ggaaggtgga gacatagggg gtgaagtcga
     7681 gcgcgccggc ggcgagggtg gcctgcaggg tctggcggcc attgttgacg taggtcaata
     7741 cgccctcggc gacattgccg tcgagttcga tgttgacgtt ggtcagcgcg atcgacgcgc
     7801 cgaccacatt ggcgcgcgct cgcagcgcga agcggccgaa cccgccgctg ccgggcggcg
     7861 cctggccggc ccagcgcaac gcgttgcgca acgatatgct gtcaacggtc agcgtgcctt
     7921 ccatcatcgt ggaggtgcgg ttggcgacgg tgccgtcgaa cgccagtttc agcggcgcgc
     7981 tggcgagccg ggccttgagg ccggatcggt cgccggaaag ggctgcgaca aagtcgctgg
     8041 cgctgatcga gccatcgacg cgttcgccgc gccagtcgaa ttgcccggtc gcggcgaagg
     8101 aacgcgagat cgacggccac gccagcgaaa ggtcgatatc tccgagcttt tcggacaggt
     8161 ggctggcggc atcctcatag ctcagcgcgc cgtcctggat tctgatttcc gaaaacgaca
     8221 cctggttatc ggcaccgggc ttcatggcgc gcgcgatggt gtcgatgaac ggcgtccagt
     8281 tgctgccgcc gccggcgtcg cgggtgacat gaatatgcgg acgcagcatc atgacgtcgg
     8341 cgatctcgaa gcgccgcaac agcagcggca gcaagcgcaa attcgcggtc aggacgtcga
     8401 cggcgagcgc agggtcggcg gtgccgccgc ccttcagccc gacgtcatga aaggagacgt
     8461 agcttccggg aaacaccgaa acgtcgatat tgcctttgat caccagatcg aggccggtca
     8521 cggcgcggat ttgcgcctcg accgcctgac gcaaggcgtc ccggtcgaga aaccaggacg
     8581 tcgagatcag gccgatcagc gccacgccga tcagcgcggc aatcggcatc cccaggcgct
     8641 tcattccttg ggccatcgtc aatgacatat ccgggtcggg ttgatgtcgt cgaatcgcgg
     8701 ttgaagtggg gcgggttgta tatcaaccaa acccgggttg gcccgatttc ggcagatatc
     8761 acagtccgga tcggcgatag ccgacatgcg gggggcccca tgacaacggg cacaacttga
     8821 ggggttttct tgacgctttc aaggccatct ggtgttcggg acctgtgcaa aagtccaatg
     8881 attgacgcct tgcgacgatt tcgcctaata atccctggca cacgcaccct cgtgcaggcc
     8941 gtaaggctcc caccgtcagg tcgcattttc atgaacaagg tctatccgga cgccaaatcg
     9001 gccctcgacg gtatcctcag ggacggcatg atgatcatgt ccggcggctt tgggctgtgc
     9061 ggcatcgccg agaccctgtc cgacgcgctg cgcgactccg gggtgaagaa cctcaccgtg
     9121 gtctccaaca atgccggcgt cgacggcatc ggattgagcc ggctgctcga gacccggcag
     9181 atcaggaaga tgatctcgtc ctatgtcggc gagaacaagc tgttcgccca gcaattcctc
     9241 gccggggaac ttgaactcga attcaatccg caggggacgc tggccgaacg gatccgcgcc
     9301 ggcggtgccg gcatccccgc tttctacacc aggaccggcg tcggcaccct gatcgccgaa
     9361 ggcaaggaag tgaaggaatt cgacggcgag aaatacatca tggagcgtgg gttgttcgcc
     9421 gatctcgcca tcgtgcacgc ctggaagggc gataccgccg gcaatctcat ctatcgcaag
     9481 accgcgcgca atttcaatcc gatgatggcg accgcggcga aagtcacggt ggccgaggtc
     9541 gagcacctgg tgccggccgg cgagctcaat cccgaccaca tccatacgcc cggcattttc
     9601 gtcaaacgca tcatcgaagt cggcaccggc aacaagcgta tcgaattccg caatacccgg
     9661 ccacgtcccg ccgcgtaagc gacccaagca ggagagcccc atggcctgga cccgtgaaca
     9721 gatggccgcg cgcgctgcca aggaattgcg cgacggctac tacgttaatc tcggcatcgg
     9781 tattcccacg ctggtttcga actacattcc cgacggcatc gacgttaatc ttcaaagcga
     9841 gaacggcatg ctcggcatgg gcccgtttcc ttacgagggc gaggaagacg ccgacctgat
     9901 caatgccggc aagcagaccg tcagcgaact gccgaccacc agctacttct ccagcgccga
     9961 ttcattcggc atggtgcgcg gcgggcatat cgatctatcg atcctcggcg cgatgcaggt
    10021 ggcgcagaac ggcgatctcg ccaactggat gatcctgggc aagatggtca agggcatggg
    10081 cggcgcgatg gacctggtgg cgggcgtcaa gcgcgtggtg gtggtgatgg agcattccgc
    10141 caaggacggc ccaaaactcc tgaaaaagtg caacctgccg ctgaccggcg agaaggtggt
    10201 cgacctggtg gtgacggatc tcgcggtctt caccatcgac aagcacggca aggacggcat
    10261 ggcgctgatc gagctcgccg acggtgtcac gctcgacgaa gtgaagtcga agaccgaagc
    10321 cgagtttcgg gtggcgctga agaacgcgtg agttagctct ccggcgcaga ccgccgcgaa
    10381 gcgggatcgg aatttttgcc agcggtcggc gctcagaacg gcttgaccac ggcaagggcc
    10441 gcgatggcgg caatcgccac caggatggcg ggcgcggcat agcacaaatg tgccggggtc
    10501 cagcgattcg cgccgcgcgc cattcggcgc aacgcgcccg actggacgcc atgcaacgcc
    10561 gataggccaa acaccaacac cagcttgatt atcagccagc gcgaggaaaa ccaccccgcc
    10621 tgaaccgcca tcgtcatgcc gagcgcccat gccagcagca tggcgggcgt agtaaccttc
    10681 cagtcccatg cccgcacaat ctcgatcatc cgcgcttctc cggcggccag cggccgggaa
    10741 gaggcgacgt gcatggcgat gaagagagac gagaccaaca tgccgccgat ccagccgatg
    10801 acggcggcga catgaaacgc cttcaaccag aggtatccca ttttctttcc ggtgaaacgg
    10861 agccggcgag cgcaagctct tttcgcttca gaaaacgttc gttcgcgaat gcgccgaaag
    10921 ggatgaacgc cacgatcacg gcacgggcaa tctccgctcg cgaccaatcc gcgcccgaga
    10981 cggtctggat caacatccag acgtagagca ggaacgccat cccgtggatg ggcccgatga
    11041 tggaggttgc cgccggatgg ccggcgatat gcttgagcgg cacagccacg aacagcagcg
    11101 caacgagagt agcgccctcc agcagcgaaa tcagacgcat ccgcctcagc tgattgcgct
    11161 cgtcggtgcg agcagttgtc tcggtcggtg gcattactcg acctcgcgga gcagcttctc
    11221 gatggccgtc agcctgatct tcatctcgga aaaatcggcc tgaatcgcag cctgcgatgc
    11281 ggcgttggcc gcttgcagcg cgaccgcctt ttccgccagc tcgcgatagg cttcctcgcc
    11341 gttggcacgc gcatgcacgg ttcgggcagc cgagaaatat ttcattccga acaccagcaa
    11401 gatggtgacg agaacaagga tcgtgaggag cagaaacgta ttcgcgtgtt cagccatgct
    11461 ggtttctccg ctaatcggat tttgaggact ggatcgatag agatttgacc gccgccgcga
    11521 tggcggttgg gctcagttca aagctgaaat ccgtggcctc aaagaagttg agcgccttgc
    11581 cgtcgccgga gagttcgagc ttgctcgtga cgagccccgc ctcttcgagc ttttgcagat
    11641 gcaggtgcag caatgggcgg ctgattccaa cgtcccgcgc gagctggctg acatagtttc
    11701 gcccccgtga acggagcgca gccacaatgc gcatgcgatg cgggttggcc agtgcctgca
    11761 gcgccctcaa cagttgatcg ccggtccgcg caatcgaggc agccattttg tcttctatct
    11821 ttcatatgta ataaatttct tacatatgaa agatagagtg tcaataaccc ttttgaaggc
    11881 ggaggaccgc ccgctatttt tcaatggatt gctcgcttac ccggagcagg ccgccgctcc
    11941 caagcgcggc gacggggcat tccgcgagaa cgcgtgccgc ccttcaaagc tgcttgagat
    12001 attcgatcag cgcccagcgt tcgtcgtcgc taaggtcctt gccgaaatcg tgcccggcat
    12061 tgctgttgcc gggctcggct cccgggccca ccacgagctt gccgtcgttg aacggcgagc
    12121 tgtcggttac gtaaccgaca ttgacgggat caaacacctt gctgccgacc atgaaggacg
    12181 gcttccttcg ttctggcggc aacagcaatt cccagaggtt cggcaccgat ccgttgtgaa
    12241 gatacggagc ggtcgcccag atgccgtgca gcacccgcga ttcataggcc ggaggcgcgt
    12301 ccggcgccag tggcacgaac atggttttga gccggtctct tacatgggat tccaggttgg
    12361 ctctcaccga gggtcgcagc agaagcacca cgatcggggt attctgtgac tcgggcaaca
    12421 ggtgtgacag atcttttctg atggcctgcc aaactccatt gttgcgattc cggaacgtca
    12481 gcgggaaggc ctcgttgatc aaggcgccaa ccactgttgt cgcgaggatg tcgtgagtct
    12541 ttgcggtctc tgccagcgtc gctccgggcg gattgggcgt cagcgcgccc gccaacaagc
    12601 cggattgcga cgaccggtcc gcgtttttga acatcctggg atccgttccg accgctttaa
    12661 ccggcgtgag ccatgtgccg tcgagcaagg gctgctcttc atgacagctg ccgcaatttt
    12721 gcgcgtagag cacgcggcct ttgtccacca gctcctgctt gagttcgaat ggccagggcg
    12781 gcggtttcaa gaccgcgatc ttttcttcga gctgctgcag accggcgaac ttcaccgagt
    12841 tgttgctgaa gtcgtgcggg ggtggcaccg cacgtttgct tggcttgaaa tcagcaaaca
    12901 cgccgaacac ctcgccggtg tttcgtccga gcgcgtggat aaagagacca ttgggaacga
    12961 agccattcca ttgcgtcttg tcctggcgcg acgcgttcca caggaaggga tagctgaccg
    13021 gcgcgtccgc gaccttgaaa ttggcgtcga tgccgaggtc tctcgccgcg acgcgattga
    13081 agatcatgcc gaaggcgtcg aggcgcccgg gcccccacgg agacgatgcc ggcagacttg
    13141 catccatgaa ggcgctgtat tgtttgaccc aggcaccgaa gtcggtcttg agagtggcga
    13201 ccttcgcagc agatgaattc gtgcccagaa cggaacgggc gaatttctca aggcgattgg
    13261 tatccttgat cgtctccttg gcggcggcct cgaggtcggt caggaaaagc tgaaaattcg
    13321 ccgtcgccgg cgcgccgtcg atgcgaagct gttgcgtggc gccgtccttt tggtactcga
    13381 tttgcgcggt gtggcacgcc gcgcaggtca tgccgaggaa gccggtcttg gcgtcgccgt
    13441 cgatcacgaa tccgaccggc aatccctccg gattcagctt ggtatcgttc ggaagatagc
    13501 cgtagcgcgc cagttgatcg ccgccgaacg gctcatccac gtcgagacgc cgcaacgcct
    13561 tgaaccaggg ataggggatc atgtgcgatc cctggcttgt cgtataaaag gcctgccgat
    13621 cttccgcggt ccagccctgc ttggcgtatt gcagcgtcgg agctgcgagg gcgccggaag
    13681 ccggctgggc gaatgtcgga tcgatggtcg agaccatcgc cgcgagcgaa aacagcagga
    13741 ccttggaaaa tgctttcatg acaatgtctc gattgatccg aagggacaaa gcctcgcgcg
    13801 ccgcagccta tatcaagggg gccgcctgct gctaccgcaa gttgtattgt gctaaaatta
    13861 tttttccggt taaaaaatgt catgcggtga ttttgatcat ctcaattcca aatcgttttt
    13921 gcaagatgct acattttgtt gatcacgctt ttcacaagcg aacagcatcg ctggatgggc
    13981 aaagacgcgc ttggcgccat cacatcaggc tgtaaccgtg gcgacgtcct ccagcccgcg
    14041 attgcgcggc tcgacgcccc aggcccacac caccacgatc tgaacgacca caaggccgat
    14101 catcagccag accacgcccg gcagctggta ggaggccatc agggcgccga cgatgaaggg
    14161 cgagaccacg gtcgcgcccc gaccgaacgt gttgcagatg ccgttggcgc gcagcctgat
    14221 ctcggtcggg aacagttccg gcgtatagac gccgaacagg atcgcggtct gcacatagat
    14281 tgcgacgatc aacacgaatc cgacactcag cacgatggcc ggatcggatg ccgcgctgaa
    14341 gcgggcatag atgtagccag cgacgatggt cacgatcgac gaaccgatga tgctcgcgcg
    14401 gcgtccgatc gcgtcggaca gataggcgcc gaccgcgcag cccaccagcg atgcgaccga
    14461 cagcaccagc gtataggcca gcgaattggc gatggtcagc ccctggcgca ggaagaactg
    14521 cggcaggaag atcacgaagc cgaagatcag tgtattgatg gtgatcagga cccagcagcc
    14581 gaccagcatg cgttgcagca gcggcggcct cagcatgtcg gatgcgctga cctgcggcac
    14641 gggggccggc accaccaccg gcggcagcgt cccgccgccg gtggattcct tttcgattgc
    14701 ctgcatcagg gcttcggctt ccgcgatgcg gccctgcgat tcaagccagc gcggcgactc
    14761 cggaagattc ttgcgcagat accagacgat cagcgagcag acgccggcaa tgatgaacat
    14821 cgggcgccag ccaaaggccg ggatgatcag atagccgagc aaagcggtca ccggggaacc
    14881 cggccaccac caggaacgcc atcatcgaca gccagcgccc gcgcgtcttc ggcggaacga
    14941 attcggtcag cgtcgaatag ccgaccacga tttcggcgcc gaggccgagg ccctgcacga
    15001 agcggcaggc gatcagctgg ttcatgtcct gcgcgaaggc cgccgccagc gacgccagcc
    15061 cgaaaatcag cagattgatc tgataggtga atcgccgccc gaacttgtcg ccgacaaatc
    15121 cggtaatcag cgcgcccagc gtcatgccga caaaggtcag cgaaatgaac tgcaggtttt
    15181 gcggaacggt ggaaaatttg gtttggacag cggacgccag cacgccaccg gcgatataga
    15241 gatcgtatcc gtcgaaaaac atgccggccc cgaccagcca gaaaacccgg tagtgaaacg
    15301 atgagatcgg gagccggtcg agacgtgcac cggcgtttac tgcttgagcc atatgaaatt
    15361 cctccccctt tgtcgccttg ttggctaatc cccgctgtcg gtcttctgtg gacgcggccg
    15421 ccgtcggtta ttttttagtt caatcgccgt tgcgtcgccg tgtcgcggta ccagtcgaac
    15481 ggctggtcgt gggtcgcgac catcacgctt ttggtgttga agtggtcgtc aaaactttcg
    15541 gtgccgaatt cgcgcccgag gccggagtca tcgacgccac cccagggtga cgccggatcg
    15601 aggcgatgat gatcgttgat ccagacgatg ccggccttga cggcggacgc cacgcgatgc
    15661 gcgcggctga tgtcgcgggt gcggatcgct gccgcgagcc cgaacggcga atcattggcg
    15721 agccgcaatg cgtcggcctc gtccttgaaa ggcgtcaccg acgtgaacgg tccgaaaacc
    15781 tcttcctgga aaatgcgcat gtcgggtttc acgtccgcga acaccgtcgg ctcgacgaag
    15841 aacccgcctt cgtggcccgg cactttggcc gcgacgccgc cggtgaccag ccgcgcaccg
    15901 tcatcgcggc cgtattgcga ataggacagg acgcggtcgc gctgccgcgc cgagatcacc
    15961 ggcccgagtt gggttgcggg atcgaaggga tcgccgatcc gaatggcttt ggtcttcgcc
    16021 ttcagcctct cgacgaattc gtcgtagatc gacgcctgca cgatatggcg cgaggcgcag
    16081 acgcaggtct gccccgcgcc gataaaggcg ccgaacgccg catagttgac ggcctgatcg
    16141 atatcgtagt catcgaatac catgaccggc gtcttgccgc cgagctccat ggtctgatgc
    16201 gcgaacactt tcgccgcagc agccccggca atgcggccag cctcggtgcc gccggtcagc
    16261 accagcttgt tgatatcgcg atgttcggcc agcatcttgc cggcgctgtg gccgaggccg
    16321 agcacgacgt tgaacacgcc gggcggcagc cccgcctcgg tgaagatctg cgccagtttc
    16381 agggtcgtca gcggggtata ttccgacggc tttacaaccg tgacgcatcc gctcgccagc
    16441 accgcggcga gcgacttgca catgatcatc aaggggtgat tgaacggcgt gcagttggcg
    16501 acgatgccga tcggggtgcg cagcgtataa ttcaggtacg agccttcgac ggggatcacc
    16561 gcgtcgcggc gcgccagcgc caatccggca aagtaccgaa agaagtccgg cagccgcgac
    16621 agctgcgcgc gggtctcgtt gagcggacgg ccgttgttga gggtttccag ccggtacagg
    16681 gattccagat tggcctcgaa ggcatccgcg aggcgattga ccagccgcgc gcgggcacgg
    16741 atgtccatgc cgccccaggt gcggccttcg aacgcggccc gcgcgctttt cattgcgcgg
    16801 tcgatgtcat cggcggtcga attcggaatc cgcgcgatca catctccggt ggcgggattg
    16861 cggacatcca gcatctggcc ggagcctgct tcgatctcgc ggccatcaat aaagttgccg
    16921 tggatttcga cgtcgacggc cgttggtttc gctgaaatgt tcatgcctct ctcccttcgg
    16981 tcatgatccg atcgatcaaa tcggatcgcg atcccatctt ttgtgcttga gcatgatctc
    17041 cgcgcaaacg cgttccgcgg ttgtcgcgag ggctcaagcg ctagacgatc accgcgttgc
    17101 gcttgagcgc cggcagcacg cgcctttcgg tttgcagaac atgcgccttg atgaggcgca
    17161 cggcgcctcg cgcatcgcgc cgctgcatcg cctcgatcag ggcgacgtgc tcggccacca
    17221 gttgcgtcgg gtcgtggcct ttcacattgg cgacgctgac gcgcaccagc cggtcggcct
    17281 gtccgatcag atcgcacagc gccgcggcca tgcggcggtt gcccgatgca tgcgccagcg
    17341 cggagtggaa ggcgcggttg taggcgatga agtcctcgtg gtcgcccgcg aaagcgcgga
    17401 attcgtcaag cgccgtcaaa acctccgctg tggcgttctc gatcgcttcc gcgacgcagg
    17461 cgggctccag cgccagccgg aaccgcaaca ggtcgcgcgc gtccgaaagc gagattggat
    17521 tgacccggta cccctgacgc ggctgtacgg tgaccagatg ctcgcgttcg agccgcagca
    17581 acgcctcgcg caccggctgg cggctgaccg cgtaacgctc ggcgagctct tgttcccgca
    17641 tatcttcgcc gggcgccaaa cggcatgtca gaatatcaga tcgcaggttt tcgtagacat
    17701 tatcgcgcag gagcatcgaa atttcctttt aatatcaatc cacttttgca gtcgtgaaat
    17761 atcacgtcaa gtcaagttgg cttgaaaagg ccggattttg aattatcaat ccaacagctt
    17821 cgacgagcgc cgcctcgcca tgcggtgccc tcccaacccg aaagagagcc ggcatgaacc
    17881 gacttttcgc caacggcacc gtccatgcga tgcagtccgg caaggggccg tttctgtttc
    17941 tgcttcattc gctgctgtcg gatcgggcga gtttcgacgc cgtcgttccc cgcttgtcgc
    18001 agtcgttccg ggtggtggtt ccggagttgc cgggttttgg tgggtccgag gccgtcagcg
    18061 gaggccttgc cgcggtcgcc gaccgaatgg ccgaggcact gacggacgcg gccggcggcg
    18121 acgatgtgtt cgtgctcggc aacggctatg gcgggttcgt ggcgctgcaa atggcgattc
    18181 gtcatcccga catcgccacc aaactcattc tcgccgattg cggtgctgcg ttctcggaag
    18241 aaggccggca agcctttcgc aatatggccg cggcatccca ggccagggga ctgtccgtta
    18301 tcaccgacgt tgcgatgcgc cggctgtttg cgccggaatt tcaggccggg catcccgacc
    18361 tgatgcacga tcgtcgcgag gcatttttga gaaccgatcc ggaagtgttc catgccgcct
    18421 gcgatgcgct ggccgcactc gacctccggc ctgagctttc gaaggtcgag gtccccgttc
    18481 tggtgctggt cggcgagcat gatgaggcga cgccgccgca gatgtcccgc gaattggccg
    18541 ccggcctgcc gcaagcaaat ctcaaaatta ttccgggctg cgcgcatgtc ccgcagctgc
    18601 aatcgccaga ggtctttctg gaggcgatcg gggatttctt gccagtggcg taaggtgcgt
    18661 atattgcggc aagcccgctg ctggtcgatg caaacaattt gagcagatcg aacgcattat
    18721 ccgttcgacg gtagctaccc tcttgcctag tcttgcccag gtttttcttg cccaaatttt
    18781 cttgcccgag gcttgaggtg tttgccggat gcacggggtt ctggactatt gtaccgggcg
    18841 aattgagcga agcttggccg ccggcgcaac cgtgatccgg gagggggaga cgaccgatca
    18901 cctctatgtc ctgatcgagg gacggcttga ggtcgtcaag ggcgagacgg ttgtcgccat
    18961 catcaccgag ccgggagccg tgctgggaga aatgtcggtt ctgctcgatc agccgcatac
    19021 cgcgacggtg cgcgcggcgt ctgactccac agtctacgag atcagcgatg ccgcatcatt
    19081 tctgcgcgag cagccggcgg tggcgctcct ggtggcgcgg ctgcttgccc agcggctcaa
    19141 tgtcgccacc acctatcttg ccgacatcat gcagcaatat gccgggcacg gcgatcattt
    19201 ggcgatggtc ggcgagattc tgcagagcat gatcaacctg ccgccgatca agatctcacc
    19261 aggttcggat cggcaatccg acccaaggat gtgagccacg ccggcggatg gctgaccggc
    19321 gcggccggcc gctgcaatgg cgcgttgagg tcgatgtatt gctcgtcgcc gagcggcagc
    19381 cagaacgcgg tattgctgtc gatatcgagc acgatatgca ccggcgggcg gccggcctga
    19441 aggccggcat tgaataccca ggtgccgccg agccgatcga accaggagcc atcggggatg
    19501 aacggcgatt ggtgcacatg gccggagatc accatcgaag gctggtagcg ctggatccac
    19561 tgcacgagtt cgacgtcgcc gaaaaagcgc ttgccgcccc aactggtcgg agaattcgcc
    19621 ggcggcgcgt gatgaaccca gatccaccgt tgcagccgcg ccgcggcagc ctcgcgcagc
    19681 tggtcgtcga tccgctgttt gaccagcggc ccatcccacc acggacagac cgtgaatagg
    19741 gtgtcgccga tcgtcaggct gtcgccgtcg caggcgatgc cgagcgcgcg aacgtcgccg
    19801 atccagcggg cgatcttctc gccttcggga ttgcgttcat cgaggtcatg atttccggag
    19861 caaagaatga cccgggtgag accggcgagc cgcgccaggt attttttcac gacgacaatc
    19921 tgggcgcgga aatcgaccgc ggatccgatg tcgagggcat cgcccgcgaa gatcacgaga
    19981 tcgaattgcg gtgccgcgct gagcaaccag tcgaactgcg gcagcgagta atgcaaatcg
    20041 gccacgatca ggcagcgcat ttcaaatcga tccggatgct caagaactgg gcgacgcggg
    20101 agggcttgta ttcaaccagc caggatcgag caaaaattgt gccgtgcctg cctgctgaaa
    20161 tgcgacagat gcccgcacga accgttctcg gcgcgatcgg gcttatcgct gtttaaagcg
    20221 cgaggacgcc ggcccccgtc aagaaaaccg caaggatttc aggctttcgc ccttgcctgg
    20281 gaaaacgtca cctcgatcag cgtgccggaa tgcgacgcgg tcttgatatg aaaatgcgcg
    20341 cggttggctt cgaccagcgc cttggtcagg gaaaggttga cgccggaacc gtcggacgcc
    20401 tggtctgacg gcgcctgggt ccggaacggc tccagggcgg ccgcgacctc gttatcgttg
    20461 agcccctggc cgctgtcgcg gacccgcagc accacctcgt cgaaatccga cagcgcggtg
    20521 gagacgatga cctggccgcc ggcattggcg agatggatcg aattgccgat caggttcagc
    20581 gcgatctggc gcaaggcgtg ggcgtcggcg atcaccggcg gcagcatctg cgccagcgac
    20641 gtccggatga tgatgcgttc gcgattggcc tgcggctgca tcaccgcgac gcattgctcg
    20701 accagatcgt tgaggtcctg gctggcaaag gtgaggtcga gcctgccggt ttcgatccgc
    20761 gacagatcga gcagatcgtt gatgacggcg atcacgcgtt cgccggagat gcggatgtcc
    20821 ttgatgtatt cgatgtagcg cttgttggcg agcctgccga aacgctcgct gatcatcact
    20881 tcggcaaagc cgatgatggc gttgagcggg gtgcggacct cgtggctgat ccgcgccagc
    20941 atatcggcct tggcgttcgc cgcgcgatcg gccaattgcc cggcctgcag caattcacgc
    21001 tcgctttttc cgctctgggc gacatcgcga aacaccgcga agaaattcgg accgtcgggc
    21061 cgggtgcgtc ccagggtcat cgacagcggg atgatgccgc ccttgcggac ccggcccagc
    21121 acgtcgcggc cgtgatcgag caggcttgca gcatcggccc ccttgatgcc gttcagatga
    21181 tccagcaccg caggctggct ttccggcgcg aacagctcaa tgagattgcg ttgcgcgaac
    21241 tcagtgccat cgtagccgaa cagcgcctcg gcgccgcggt tgcaaaaatt gatgtggcca
    21301 tcggcatcga acatcacgat gccttcggcg gtggcgtcga ggatggcgcc aagttcctcg
    21361 gcattggcct ggctcacggc cgacggtgcg cggaagggga tgaattcggg aagcacggcg
    21421 accgcggcgg cggcctcgtt gcgcgtgccc gagaaaatca gagccagcgc ggaatcgccg
    21481 tcccatgaaa tcgtgaacag gcgcgcgtcg atcgccggcg caccttcgga ggaagcctcc
    21541 gttccggaaa tcgtcaccgg cctgccggtg tccgatgtgc tgctggcgct cgagacgccg
    21601 ggctcgacat agagcgcgtc gagacctccg gcatcttcca gtgcgt
//
LOCUS       DATNOI010000676         5332 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_754304,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000676 DATNOI010000000
VERSION     DATNOI010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754304"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1469)
                     /locus_tag="VN968_27145"
     CDS             complement(138..1469)
                     /locus_tag="VN968_27145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HXL32942.1"
                     /translation="MFGHPKGLSFLFATEMWERFSYYGMRALLVLYMVDYLLKPEIAH
                     DVWGLATLKRGLEAVSGPLGIQPLASQIYGLYTGLVYLTPIFGGILADRWIGRTPTVV
                     LGAALMVAGHFMMAFEHLFLLALVLLILGNGAFKPNISTQVGGLYAPDDSRRDRAYSV
                     FYVGINVGAFFSPLVCGSLGENVGWHYGFASAGVGMAIGLATYLAGLPRLPKQQFQDP
                     PVEAGKPANPDLTKSLLGLAILLIPSTLFWAAYEQQGNTIALWIEHSTDRRLNLLFWQ
                     GEIPVTWFQAINPLMIFAFTPPLIACWSRWAKVGREPSTIGKMSLGCMLLSLAYLVMA
                     VAAWSGAPASASSLWLLLYFAVLTIAELYFSPIGLSLVSNLASNKSRSTVMGLWFTTS
                     FVGNMAAGWIGSFWSTLSNAGFFLLVAALGAVAAIVIHLAQPVLRSLLSNA"
     gene            complement(1633..2286)
                     /locus_tag="VN968_27150"
     CDS             complement(1633..2286)
                     /locus_tag="VN968_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL32943.1"
                     /translation="MATRPRADAADDPPARERILEAAFSAFMESGFAEASMLEIATRA
                     RVSKRELYTLVGSKQDMLVACIAARAKRLQAPADLPAPQDRKTLAQVLTAFGAQLLRE
                     TSDPTVVAVFRLAIAEAVRAPEVARALTSAGIETSRAALRDIMGSAQSAGLLGGQPAE
                     MTEQFAGLLWGNLTMNLLLRVADRPSPREITRRAHDATVAFLKIYPQPDQAGTGRSA"
     gene            2386..3303
                     /locus_tag="VN968_27155"
     CDS             2386..3303
                     /locus_tag="VN968_27155"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HXL32944.1"
                     /translation="MAKPVLVTSAAGGRQGKTGRHVSEMLLKRGIPVRAFVHKIDERS
                     EALRAQGAEIVEGDFLDVRSVKRAVEGTSSIYFAYPVQDGLLDATAAMAVAAREAGIS
                     RLVNLVMLQSSVDAPTPRMRQNYLSEQVFEWAGIGAVHVRATVFYENLGSQVRLSLPA
                     QGAVRLPWGKEHTVLPLVAAEDVARVAVGLLTSPSLTAGTAYPVIGTVISLKEIIATF
                     GRVLGKDVRYEEISDDEWRRDALARGYNAHAVEHLSALWKALRAAGLDPQTARFAVTD
                     TIEKLGGAGPKTFEAFVREGQGELLAKTA"
     gene            3437..4165
                     /locus_tag="VN968_27160"
     CDS             3437..4165
                     /locus_tag="VN968_27160"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXL32945.1"
                     /translation="MFVLLITVATHCEGGLVSTQKILGIGVGLAGVLVVMGAEALRGV
                     GTAAPLAPSAVLAASLCYALAPSAVLAASLCYALAPMWARRFAQFPAILTAAGSMSCA
                     ALLMLPASLVIDRPWTPVPTPAAVGAVAVLAVLCTAIAMIVYFRLVRTLGALATTSGS
                     YLRAGFSVALGILFLGESFTWSTLAGMVLIIAGVVAVTAQLRWPWPRRNADDRISHPP
                     RAGPNVSLAMDSRRPRPPAPLQAF"
     gene            complement(4212..5225)
                     /locus_tag="VN968_27165"
     CDS             complement(4212..5225)
                     /locus_tag="VN968_27165"
                     /EC_number="3.5.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008660 -
                     1-aminocyclopropane-1-carboxylate deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0042217 - 1-aminocyclopropane-1-carboxylate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate deaminase"
                     /protein_id="HXL32946.1"
                     /translation="MLEKFERYPLTFGPTHIEKLERLSQHLGGKVELYAKREDCNSGL
                     AFGGNKLRKLEYIVPDAIASNADTLVSIGGVQSNHTRMVAAVAAKIGMKCRLVQESWV
                     PHEDAVYDRVGNILLSRIMGADVQMVDEGFDIGIRESWEAAIADVKTKGGKPYAIPAG
                     ASVHKYGGLGYVGFAEEVRAQEKELGFAFDYIVVCTVTGSTHAGMLVGFARDGRERKV
                     IGIDASFTPAQTKAQVLEIARNTAKLVELGKEIVEDDVILVEDYAYPVYGVPSQETKD
                     AIRLCARLEGMITDPVYEGKSMQGMIDLVKKGYFPEGSKVLYAHLGGAPAINGYAYTF
                     RNG"
BASE COUNT          994 a         1664 c         1673 g         1001 t
ORIGIN      
        1 ctcgatctcg ccgacaaggc cgagcgaacg ctgttcgtca aggacctcgc gattgccggc
       61 gcgctgatgg tcatcgcggc cggcgcgtcg caaattcgcg cggtcgcatc gatcggttag
      121 tccaagccgg atccgtccta cgcgtttgac agcaaactgc gcaggaccgg ttgcgcaaga
      181 tggatcacga tcgcggccac agcgccaagg gccgcgacga gcagaaagaa accggcattc
      241 gacaaggtgg accagaagct gccgatccag ccggcagcca tatttcccac aaaactcgtg
      301 gtgaaccaaa gccccatcac tgtcgagcgc gacttgttgg acgccaggtt tgacaccagc
      361 gagaggccga tcggcgaaaa atagagttcg gcgatggtga gcaccgcgaa atagagcagc
      421 agccagagcg aactggctga ggcgggcgcg ccggaccacg cggcgacggc catcaccagg
      481 taggccaggc tgagcaacat gcagccaagc gacatttttc cgattgtcga tggttcgcgc
      541 cccaccttcg cccatcgcga ccagcacgcg atcaagggtg gcgtgaaggc gaagatcatc
      601 aggggattga tcgcctggaa ccaggtcacc ggaatttcgc cctgccagaa cagcaagttc
      661 agccggcgat cggtcgagtg ctcgatccaa agcgcgatgg tgtttccctg ttgttcgtag
      721 gcggcccaga acaacgtcga gggaatgagc aggatcgcca agccgagaag cgacttcgtc
      781 aagtccggat tggccggctt ccccgcttcg acaggcggat cttgaaattg ttgtttcggc
      841 aagcggggaa ggccggccag gtaagtcgca agcccgatcg ccatgccgac gcccgcgctg
      901 gcaaagccgt agtgccagcc gacgttttca ccgagagaac cgcagaccag cggcgaaaag
      961 aacgcgccaa cgttgattcc gacgtagaaa accgagtaag cgcggtctcg gcgcgaatcg
     1021 tcgggcgcat aaagaccgcc aacctgcgtc gaaatattcg gcttgaaagc gccgttgccc
     1081 agaatgagaa gcaccagcgc gagcagaaaa agatgttcga aggccatcat gaaatggccc
     1141 gcgaccatca gcgccgcgcc aaggacgacg gttggggttc ggccgatcca tcggtcggcg
     1201 agaattccgc cgaatatcgg ggtcagatag acgaggccgg tgtagagccc ataaatctgc
     1261 gatgccagcg gctggatgcc aagcggtccg gatacggctt cgagtccccg cttcaacgtc
     1321 gcaaggcccc atacgtcatg agcgatttcc ggcttcaaca gataatcgac catgtagaga
     1381 acgagcaacg cccgcattcc gtaataggaa aagcgttccc acatctccgt tgcgaacaga
     1441 aacgaaagac cttttgggtg gccaaacaac tgatcgccgc gttcgccgcc gcgacccggg
     1501 tcgctcgatg gtggattgct ctgggctatg acgctctcct agcggaccgc agcgacgccg
     1561 tcccaatgtc ttcgatccca ttgctgttgc ggagtttatc accgggcgcg gattcgcgcg
     1621 acctgttggc ggttacgcgc tacgccccgt cccggcttgg tccggttgcg gatagatttt
     1681 caggaaggcg acggtggcgt cgtgggcacg ccgcgtgatc tcgcgggggc tcggccgatc
     1741 ggcgacgcga agcagcaggt tcatcgtcag gttgccccac aacagcccgg cgaattgctc
     1801 ggtcatctcg gcaggttggc cgccgaggag tcctgccgac tgggcgctcc ccatgatgtc
     1861 tctcaaggcg gcacggctgg tctctattcc ggccgaggtc agggcccgtg cgacctcggg
     1921 cgcgcgtacc gcttcagcga tcgccaaccg gaataccgcg acgacggtgg gatcgctggt
     1981 ttcgcgcagc agctgcgcac cgaacgccgt cagcacctgc gcgagggttt tccggtcctg
     2041 cggcgcgggc aggtcggccg gtgcctgcag ccgcttggcg cgcgcggcga tgcaggccac
     2101 cagcatatcc tgtttgctgc cgaccagcgt gtaaagctcg cgcttggaga cgcgcgcgcg
     2161 ggtcgcgatc tcaagcatgc tggcctcggc aaatccgctc tccataaacg ccgagaaggc
     2221 tgcctcgagg atgcgctcgc gcgccggggg gtcgtcagcc gcatccgcgc gcggtcgggt
     2281 cgccatcatg tttggccttg acatggtacc aatcggtacc ataagttcgg agcaatcggt
     2341 accggatggt accaacccag gtcgaaatgt aaaggagcca tagccatggc aaaaccagtt
     2401 ctcgtcacgt cagcggccgg cggccggcaa ggcaagaccg ggcggcacgt atccgaaatg
     2461 ctgctcaaac gcgggattcc cgtccgcgcc ttcgtccaca agatcgacga acgctcagag
     2521 gcgctgcggg cccaaggcgc cgaaatagtc gaaggcgact tcctcgacgt ccgctccgtc
     2581 aagcgcgcgg ttgagggcac ctcgtcgatc tattttgcct atccagtgca ggacggcctg
     2641 ctcgacgcga ccgcggccat ggcggtcgcc gcgcgcgaag cgggtatctc ccgtctcgtc
     2701 aatctggtga tgctgcaatc gtcggtcgat gccccgaccc cgcggatgcg gcagaactat
     2761 ctgtccgaac aggtgttcga atgggccgga atcggcgcgg tgcatgtcag ggctacggtg
     2821 ttctacgaga atctcggctc ccaggtgcgc ctgagcctgc cggcgcaagg ggcggtgcgg
     2881 ttgccctggg gcaaggaaca caccgtgctt ccgctggtcg ccgccgagga cgtcgcgcgc
     2941 gttgccgtcg gcctcttgac cagcccctcc ttgacggcgg gcaccgccta tccggtgata
     3001 ggcacggtca tttcgctcaa ggagatcatt gcgaccttcg ggcgcgtgct gggcaaggac
     3061 gtgcgctatg aagaaatttc ggacgacgaa tggcgccgcg atgcgctcgc gcgcggctac
     3121 aatgcgcacg cggtcgaaca tctctccgcg ctctggaagg cgctccgcgc cgccggcctc
     3181 gacccgcaga ccgcacgctt cgcggtgacc gatacgatcg aaaaactcgg cggcgccggg
     3241 cccaagacgt tcgaggcgtt cgtccgcgag ggacagggcg aattgttggc gaagacagcc
     3301 tgacccaggc atgatcgagg ccgatgcgtg cagcgatcga tttgccggag tttttcggcg
     3361 ctgattgtcc tgcagtcgct ctggtgagga gcatatcgcg agcggccttg ccggcgtgct
     3421 caatgccacg ccacccatgt tcgttctctt gatcacggtc gccacccact gtgagggtgg
     3481 gcttgtcagc acccagaaaa ttcttggcat tggcgtcggt cttgcgggcg tgcttgtcgt
     3541 tatgggcgct gaagcgctgc gcggcgtcgg cacggccgca ccgctggcgc cgagcgcggt
     3601 gctcgcagcg agcctttgct acgcgctggc gccgagcgcg gtgctcgcag cgagcctttg
     3661 ctacgcgctg gcgccgatgt gggcgcggcg ctttgcccaa tttccagcga ttctcacggc
     3721 agcgggctcg atgagctgcg cagccttgct gatgctgcca gcttcgttgg tgatcgatcg
     3781 gccttggacc cctgttccga cacctgccgc cgtcggcgct gtggctgttc tcgccgtcct
     3841 ctgcacagcg atcgccatga tcgtctattt ccgtctggta cgcacgctcg gcgcccttgc
     3901 cacgaccagt ggcagctatc tgcgggcggg tttctctgtt gcactcggca tcctgtttct
     3961 cggagagagc ttcacctggt cgacccttgc cggaatggtg ctgatcatcg ccggcgtcgt
     4021 cgccgtgacc gcgcaacttc gctggccatg gccaaggagg aatgcagatg accgtatatc
     4081 tcatccacct cgggctggcc cgaatgtgtc attggcgatg gattcgagac gaccacgacc
     4141 gcccgccccc ctgcaagctt tctgagatcg agcacggggt cgcgagcgct caacgacgta
     4201 ttggccgccg gtcaaccgtt gcggaatgta taggcgtagc cgttgatcgc cggtgcgccg
     4261 ccgagatggg cgtagagaac ttttgaccct tcggggaaat agcccttttt cacgaggtcg
     4321 atcatgccct gcatggattt accttcatag acgggatcgg tgatcatgcc ttcgaggcgc
     4381 gcgcaaagcc ggatcgcgtc tttcgtctcc tgcgacggaa cgccatagac cgggtaggcg
     4441 taatcctcga ccagaatgac atcgtcttcg acaatctcct tgccgagctc gacgagtttt
     4501 gcggtgttcc gggcgatctc aagcacctga gctttggtct gcgccggtgt gaacgacgcg
     4561 tcgatgccga tcaccttgcg ctcccgcccg tccctggcaa aaccgaccag catgccggca
     4621 tgggtcgaac cggtgacggt gcagacgacg atatagtcga aggcaaagcc gagttctttt
     4681 tcttgcgcgc gcacctcctc ggcaaagccg acataaccga gaccgccata tttgtgcacc
     4741 gaggcgcccg ccgggatcgc gtaaggcttg ccgcccttgg tcttcacatc cgcgatcgcc
     4801 gcctcccagc tttcccggat cccgatgtcg aatccctcgt cgaccatctg gacatcggcg
     4861 cccatgatcc ggctcagcag gatattgccg acgcggtcat agaccgcgtc ctcatgcgga
     4921 acccagctct cctgcaccag acggcacttc atgccgatct tggccgcaac ggctgcgacc
     4981 atgcgggtat ggttggactg cacgccgccg atcgagacca gcgtatcggc attggaggcg
     5041 atcgcatcgg gaacgatata ttcgagcttg cgcagcttgt tgccgccgaa ggcgaggccc
     5101 gaattgcagt cctcgcgctt ggcatagagt tcgaccttgc cgccgagatg ctgcgagagc
     5161 cgttccagct tttcgatatg ggtgggcccg aatgtgagcg gatagcgctc gaatttctcc
     5221 agcatggtca tccccgtttg atggtcctga tctcaggaac gccctatcac cgggggctgg
     5281 aaaggtgctg tctaaatcgg cggcaattcc ttatatagat tgtacaaata tc
//
LOCUS       DATNOI010000677         6715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2056676,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000677 DATNOI010000000
VERSION     DATNOI010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2056676"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VN968_27170"
     CDS             complement(<1..1070)
                     /locus_tag="VN968_27170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HXL32947.1"
                     /translation="MATKAKTLQVKDKEKDDKAADAPEKDSPDAPSPLLDLSDAAVKK
                     MIKQAKKRGFVTFDQLNEVLPSDTTSPEQIEDIMSMLSDMGINVSEAEDSDSDEESKE
                     EAEDDTDNELVEVTQKAVTEVKKSEPGERTDDPVRMYLREMGTVELLSREGEIAIAKR
                     IEAGREAMIAGLCESPLTFQAIIIWRDELNEGKIFLRDIIDLEATYAGPDAKNNMNPS
                     LLAAPLGPDGQPLAAVNGNGATAAMPAQPAPAHIAPPAAPPAPTPFRPAPASAPAAAA
                     EGESTSEPAADSDMDDDEFENQMSLAAIEAELKPKVVETFDKIADNYKKLRRLQEQDI
                     SNQLQNETLSPAQERKYKKLKD"
     gene            complement(1418..3451)
                     /gene="dnaG"
                     /locus_tag="VN968_27175"
     CDS             complement(1418..3451)
                     /gene="dnaG"
                     /locus_tag="VN968_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HXL32948.1"
                     /translation="MRFTPQFLDELRARLPVSEVVGRRVKLKRAGREWKGLSPFQQEK
                     SPSFTVNDQKGFYHDFSSGKHGDIISFLMETEGVAFTEAVERLAQMAGMPLPAATPDA
                     ARHEQRRKTLYDVMDLAAKFFCSTLASRNGAKARGYLGDRAISPAVQLQFGIGYAPGE
                     RFALKEHLGALGIPVEDMVEAGLLIAGDDIPVPYDRFRDRVMFPISDLRGRVIAFGGR
                     ALEKDVAAKYLNSPETPLFHKGDNLYNLAPARLAAHNGGALVVVEGYVDVIAMVGAGF
                     PASVAPLGTALTENQLALLWKMADEPILCFDGDKAGQKAAWRAADMALPHLLPGKSLR
                     FALLPEGQDPDDLARSGGRGAIEEVISAARGLADVIWSREIEGGTFATPERRAALEKR
                     INELTNGIRDEVVRRYYRQDLAERLQRTFAPEGGRGGYGRGSFRGGAGNFGRESGRAF
                     SARGSFTPGAAGRSEPSRGGRPQGSAGNQTINRGPYQVASPQLALSPIMRGQRSAISR
                     REALILQSLINHPWLLHDHLEEVAALELAHPEAHKLRAGIIAAFANDHHHSPEPAEQS
                     EKMRADLAAGGFSQSLQRVDLAITTAAVWGAKGSAAREDVLSTWHQLVSLHRQWHSLL
                     RELKDAEFALGDEASEANLTWLRDVKARLAEVDGTEALIEGFGESSGRFQRSV"
     gene            complement(3564..3959)
                     /locus_tag="VN968_27180"
     CDS             complement(3564..3959)
                     /locus_tag="VN968_27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HXL32949.1"
                     /translation="MTSNNLFVYGTLMRGFDHPMAQLLSRGADLIGEARCQGRLYMVT
                     HYPALVLSGDPADVVFGELYRLRAPDELLREFDMYEACGEGFAEPTEYVRQMLSVTLE
                     GGATEAWTYVYNWPVAHLTRIASGRFLER"
     gene            complement(4089..4901)
                     /locus_tag="VN968_27185"
     CDS             complement(4089..4901)
                     /locus_tag="VN968_27185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HXL32950.1"
                     /translation="MEIKVGFEIAHTAVQPTPMVIMLSIHPSRYSDIIGTESIVAEPN
                     VPIGFYRDSFGNVCGRLVAPAGGVTLRGSALVRDSGLPDAVAPTAQQLPIDQLPDDLL
                     VYLMASRYCETDKLTDIAWSLFGNTPPGWGRVAAICTYVHNHVTFGYEHANYMKSAHD
                     VYEQGAGVCRDFAHLALTFCRCMGIPARYCTGYMGDIGIPPDPAPMDFSGWFEVYLSG
                     QWYTFDARHNHPRIGRILMGTGRDAADVALTTSFGRMDLVKFVVISDEVVAA"
     gene            complement(4903..5793)
                     /locus_tag="VN968_27190"
     CDS             complement(4903..5793)
                     /locus_tag="VN968_27190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HXL32951.1"
                     /translation="MPLLTIHHKTEYRYAHPVAFGEHRIMLRPRDGHDLRVLSGSLEI
                     VPEPMSLRWIHDVFGNSVAIATFDERAETLTFSSTATVEHNPAEEYAPTPDDPAYFYP
                     FLYSDDEFPDLVQFITPQYGDPDGALSAWARNFLDEEGPTPTFNILSDMTHGIRKAFT
                     YRKRHEHGTQHPLDTLQTRSGTCRDYALFMIEALRRLGIAARFVSGYLFIPGDRSHGY
                     VGGGSTHAWVQAYLPSAGWIEFDPTNGIVGTKDLIRVAVARDPRQAVPLHGTYLGSAD
                     AYVGMEVSINVVSVDVGAEA"
     gene            5894..6697
                     /locus_tag="VN968_27195"
     CDS             5894..6697
                     /locus_tag="VN968_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HXL32952.1"
                     /translation="MALDTGGDTSLLLGSTDVPPVWEDNAAGGSPFLLTCDHYGRLTP
                     QSLGDLGLPASELTRHIAWDIGIAGVAESLSRQLGAHLIAQRYSRLVIDCNRPPGAPG
                     SIPRISEATTIPANEALGRDAAELRRKAIFDPYHRRIAEVIDARLREGVPTVLVSLHS
                     FTPVYAGIARPWHIGTLYQRDTRLPRLLLKQLRGEGDLVVGDNEPYAVSDETDHTIPV
                     HGEARGLMNTGIEIRQDLIADPAGQHQWADRLARIFGEIETALRGQRLV"
BASE COUNT         1194 a         2149 c         2121 g         1251 t
ORIGIN      
        1 tcgtccttca gcttcttgta tttgcgctcc tgcgccggcg acagcgtctc gttctgcagc
       61 tgattggaga tatcctgttc ctgcaggcgg cgcagcttct tgtagttgtc ggcgatcttg
      121 tcaaaggttt cgaccacctt cggcttcagc tcggcctcga tggcggcgag cgacatctgg
      181 ttttcgaact cgtcgtcgtc catgtcggaa tcggcggcag gttccgaagt gctttcaccc
      241 tcggcggccg cggccggtgc tgatgctggt gctggacgga acggggtcgg cgccggcggg
      301 gcggccggcg gcgcgatatg cgcgggcgct ggctgtgccg gcatcgcggc ggtcgcgcca
      361 ttgccgttga cggcggcgag cggctggccg tcggggccga gcggggctgc gagcagcgac
      421 gggttcatgt tgttcttggc gtcgggaccg gcgtaggtcg cttccagatc gatgatgtcg
      481 cgaaggaaga tttttccctc gttgagctcg tcgcgccaga tgatgatggc ctggaacgtc
      541 agcgggcttt cgcagagccc tgcgatcatc gcctcgcggc cggcctcgat gcgcttggca
      601 atggcgattt cgccctcgcg cgacaacagc tcgacggtgc ccatctcgcg cagatacatg
      661 cggacgggat cgtcggtgcg ctcgccgggc tcggatttct tgacttcggt gacggccttt
      721 tgggtgacct cgaccagttc gttgtcggtg tcgtcctcgg cctcttcctt gctttcctcg
      781 tcgctgtcgg aatcctcggc ctcggagacg ttgatgccca tgtccgagag catcgacatg
      841 atgtcctcga tctgctccgg cgaggtggtg tcggagggca agacttcgtt gagctgatcg
      901 aaggtcacga agccgcgctt cttggcctgc ttgatcatct tcttgacggc ggcatccgac
      961 aggtcgagca acggcgacgg cgcgtccgga gaatccttct caggggcgtc cgctgccttg
     1021 tcgtcctttt ccttgtcttt tacctgcagc gtctttgcct tggtggccat tcattgctcc
     1081 cgaaacgcgc tcatgcagga agcgtcaccg cgcccgcctt gaatactgaa ccgacttatg
     1141 acgcggaaag ggtggcgcat acttgtcgcc accccctgcc catgtcactg gatttcgtac
     1201 tattagcccg gcgcgtattc cgcaaaagtg aatatcggtt ttgcgatcag aatacgcgcc
     1261 agatttttct gacgcatttt cgaacggcga accggtctcc acttcgcctc aaaatgctac
     1321 ggacccgtta agcttcgatt aacctgtttt tgccgccaaa ccacgggttt ggcgagtctt
     1381 tttgcggctc cggacaggcc gaccgcatga atttcaatca aacgcttcgc tggaaccggc
     1441 ccgacgactc gccaaaaccc tcgatcaatg cctcggtgcc atccacttcc gccagccggg
     1501 ccttgacgtc ccgcagccac gtcaaattag cctcgctggc ctcgtcgccc aaggcgaact
     1561 cggcgtcctt caattcccta agtaatgagt gccattggcg atgcaaggag acgagctggt
     1621 gccaggtgga aagaacgtcc tcccgcgccg cggagccttt cgccccccat accgccgcgg
     1681 tcgtgatcgc cagatcaacc ctttgaagtg attgagaaaa tcctcccgcc gcgaggtcgg
     1741 cgcgcatctt ctcgctctgc tcggcgggtt ccggactgtg gtggtggtca ttggcgaagg
     1801 cggcaatgat gccggcgcgc aacttatgcg cctccgggtg ggccaattcg agcgcggcga
     1861 cctcctccag atggtcgtgc agcagccagg gatggttgat cagggactgc aggatcagcg
     1921 cctcgcggcg ggagatcgcg ctgcgctggc cgcgcatgat cgggctcaat gccagctgcg
     1981 ggctcgccac ctgatagggc ccgcggttga tggtctggtt gcccgcagat ccctgtggac
     2041 ggccgccgcg cgagggctcg gaccggcccg ccgcccccgg ggtgaacgat ccacgcgcgg
     2101 aaaacgcccg gcctgattcg cggccgaaat tgccggcccc cccgcgaaaa ctgccccggc
     2161 catagccgcc gcgcccacct tccggcgcga acgtccgctg caaccgttcg gcgagatcct
     2221 ggcgataata gcgccgcacc acctcgtcgc ggatgccatt ggtgagctca ttgatccgct
     2281 tttccagcgc cgcgcgccgc tccggcgtcg caaacgtgcc gccttcgatt tcgcgcgacc
     2341 agatcacgtc ggcgaggccc cgcgccgccg agatcacctc ctcgatcgcg ccacgcccgc
     2401 cggagcgcgc gagatcgtcg gggtcctgtc cttccggcag cagcgcgaac cgcaggctct
     2461 tgccgggcaa cagatgcggc agcgccatgt cggcggcgcg ccacgccgcc ttctgcccgg
     2521 ccttgtcgcc gtcaaagcaa agtatcggct cgtccgccat cttccataac agcgcgagct
     2581 ggttttccgt cagcgcggtg ccgagcgggg cgacgctcgc cggaaaaccg gcgccgacca
     2641 tggcgatgac gtcgacatag ccttccacca ccaccagggc gccgccgtta tgggcggcaa
     2701 gccgcgccgg tgccaagttg tagagattgt cgcccttgtg aaacagcggg gtttccggcg
     2761 aattcaggta ttttgccgcg acatcttttt ccagcgcgcg gccgccgaac gcgatcaccc
     2821 gtccgcgcaa atcgctaatc ggaaacatca cgcggtcgcg aaaccggtca tacggcaccg
     2881 gaatgtcatc gcccgcgatc agcaggccgg cttccaccat gtcctctacg ggaatgccga
     2941 gggcgccgag gtgctccttc aaggcaaacc gttcgccggg cgcatagccg atgccaaact
     3001 gcaattgcac cgccggcgaa atcgcgcggt cgccgagata gccgcgcgcc ttggcgccgt
     3061 tgcgcgaggc cagcgtgctg caaaagaact tcgcggcaag gtccatcacg tcatacagcg
     3121 tcttgcggcg ctgctcgtgg cgcgccgcgt cgggggtggc ggcgggcagc ggcatcccgg
     3181 ccatctgtgc gaggcgctcg acggcttcgg tgaaagcgac gccctcggtc tccatcagga
     3241 aggagatgat gtcgccgtgc ttgccggacg aaaaatcgtg ataaaatccc ttctggtcat
     3301 tgaccgtgaa cgacggcgac ttttcctgct gaaacggcga caggcccttc cattcgcgcc
     3361 ccgccctctt caacttcaca cgccggccca cgacttccga aaccggaagc cgggcgcgca
     3421 gctcgtcgag gaattgaggc gtaaagcgca taagggattt tcggtgagtc gggggcggtt
     3481 gccaagcgat catggataca ggggcggctg gctaaaatac gagaattatt gggattctcc
     3541 cggctattca caggggcaat tcactatctc tccaaaaacc gccccgaggc gatgcgcgtc
     3601 aggtgcgcga ccggccagtt gtagacatag gtccaggcct ccgtcgcgcc gccctcgagc
     3661 gtcaccgaca gcatctgcct gacatattcg gtcggttcgg caaaaccctc gccgcaggcc
     3721 tcgtacatat cgaactcgcg cagcaattcg tcgggcgcac gcagccgata gagttcgccg
     3781 aacacgacgt ccgcggggtc gccggacaac accagcgcgg gataatgcgt gaccatatag
     3841 agccggccct ggcaccgcgc ctcgccgatc aaatccgcgc cgcgcgacaa gagctgcgcc
     3901 atcggatggt cgaagccgcg catcagcgtg ccgtagacga agagattgtt ggatgtcatg
     3961 cggctattac tatcccgtca ttccggggcg atgcaaagca tcgaacccgg aatctcgaga
     4021 ttccgggtct ggtgctaacg caccatcccg gaatgaccgg tgcagaactc gcaatgacga
     4081 aaacagcatt acgccgccac gacctcgtcg ctgatgacaa caaacttcac cagatccatt
     4141 cgcccaaagc tcgtggtcag cgccacgtcg gcggcgtcgc ggccggtgcc catcaggatg
     4201 cggccgatcc gcggatgatt gtggcgggcg tcgaaggtgt accactggcc ggacagatag
     4261 acctcgaacc agccggaaaa atccatcggt gcgggatccg gcggaatgcc gatgtcgccc
     4321 atatagccgg tgcaatagcg cgccgggatg cccatgcagc ggcagaaggt cagcgccaga
     4381 tgcgcgaaat cgcgacagac gccggcgccc tgctcgtaga cgtcatgcgc ggatttcatg
     4441 tagttcgcat gctcgtagcc gaaggtgacg tggttgtgca cgtaggtgca gatcgcggcg
     4501 acccggcccc agcccggcgg ggtgttgccg aacagcgacc aggcgatgtc ggtcagcttg
     4561 tcggtttcgc aatagcggct cgccatcagg tagaccaaga ggtcgtcggg cagctgatcg
     4621 atcggcagct gctgcgcggt cggcgccacg gcgtcgggca atccggaatc gcgcaccagc
     4681 gcgctgccgc ggagcgtcac gccgccggcc ggcgccacca gcctgccgca gacattgcca
     4741 aagctgtcgc ggtagaatcc gatcggcacg ttgggctcgg cgacaatgct ttcggtgccg
     4801 atgatgtcag aatagcgcga cgggtgaatg ctgagcatga tcaccatcgg cgtcggctgc
     4861 acagccgtgt gcgcgatctc gaagcctacc ttgatttcca tctcaagcct ccgctcccac
     4921 gtccaccgaa acgacgttga tgctgacctc catgccgaca tacgcatcgg ccgaaccgag
     4981 ataggtgccg tgcagcggga cggcctggcg cggatcgcgc gccaccgcca cgcgaatgag
     5041 atccttggtg ccgacaatgc cgttggtggg atcgaactcg atccagccgg cgctcggcag
     5101 gtaggcctga acccaggcat gggtcgagcc gccgccgaca tagccatgcg accgatcgcc
     5161 cgggatgaac agatagcccg acacgaagcg cgcggcgatg ccgaggcgcc gcaacgcttc
     5221 gatcatgaac agcgcatagt cgcggcaggt gccggacctg gtctgcaggg tgtcgagcgg
     5281 atgctgggtg ccgtgctcat gccgcttgcg gtaggtgaac gccttgcgga tgccatgggt
     5341 catgtcgctg agaatattga acgtcggggt cggcccctcc tcgtcgagaa aattccgcgc
     5401 ccacgccgac agcgcgccgt cgggatcgcc atattgcggc gtgatgaatt gaacgaggtc
     5461 gggaaactcg tcgtcgctgt agagaaacgg gtagaaatac gccggatcgt ccggggtcgg
     5521 cgcgtactcc tcggccggat tgtgctcgac ggtcgcggta gagctgaacg tcagggtttc
     5581 ggcgcgctcg tcgaaggtcg caatcgcgac gctgttgccg aacacgtcat ggatccagcg
     5641 cagcgacatc ggctccggca cgatctcgag actgcccgac agcacccgca ggtcatggcc
     5701 gtctcgcggg cgcagcatga tgcgatgttc gccgaacgcc accgggtggg catagcgata
     5761 ttcagtcttg tgatgaatcg tcagcagggg catcgtcgaa gcaatcatcg tgattttgcg
     5821 caatctaatc tataccgatc cctgcttatt tcgaaatcgg tatgctgctt cgctaggcaa
     5881 tagttgagga aaaatggcct tggacactgg cggggacaca tcattactcc ttggaagcac
     5941 agatgttccg ccggtttggg aagataatgc cgcgggcggc tcgccgttcc tcctgacctg
     6001 cgatcattac ggccggctga ccccgcagag cctcggcgat ctcgggctgc cggccagcga
     6061 gttgacgcgg catatcgcct gggatatcgg gattgccggc gtcgcggagt cgctatcgag
     6121 gcagctcggc gcgcatctga tcgcgcagcg ctactcgcgg ctggtgatcg actgcaatcg
     6181 cccgcccggc gcgccaggtt cgatcccgcg catcagcgag gcgaccacga taccggccaa
     6241 cgaagcgctt ggccgcgatg cggctgagct gcggcgcaag gcgatcttcg atccctatca
     6301 tcgccgcatc gccgaggtca tcgacgcgcg gctgcgcgaa ggcgttccga cggtgctggt
     6361 gtcgctgcat agttttacgc cggtctacgc cggcatcgcc cggccctggc atatcggcac
     6421 gctctatcaa cgcgataccc ggctgccacg gctcctgctc aagcagcttc gcggcgaagg
     6481 cgatctggtg gtcggcgaca acgagcccta tgcggtgagc gacgagaccg accacaccat
     6541 ccccgtgcac ggcgaggcgc gcggcctgat gaacacggga atcgaaattc gccaggacct
     6601 gatcgccgat ccggccggcc agcaccaatg ggcggatcgg ctggcacgaa tttttggcga
     6661 gatcgaaaca gcgttgcgcg ggcagcggct ggtatagctg cgaaccgcgt caccg
//
LOCUS       DATNOI010000678        10897 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_898808,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000678 DATNOI010000000
VERSION     DATNOI010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10897
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898808"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1922)
                     /locus_tag="VN968_27200"
     CDS             complement(<1..1922)
                     /locus_tag="VN968_27200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HXL32953.1"
                     /translation="MSGVIVAMRRTLLSCTSLARNGLIGLGAAALMSIAPAHAGDLPF
                     QHAISVSLDLNRQEIAVLATALALLGFSVVAAVLLMRTRVRATRNEARLRSDIGDLQV
                     QADRLRALLFAEPQILISWAAGDDRPQISGDTSLLMPQESQLHRPQRILAFGTWLPPE
                     PALQIDHAVDALRDAGEGFLLNLTTSAGHAVEAMGRAIGGQAVVRIRALSGLRRELAE
                     LNVRHKALLGETEILRAFVAAVPWPIWAKRAKGGLSYANAAYARATEATGIADAIDRD
                     LELLDSGDRHEMDRALNEKAAFTARLPIVIGGERRIYDVHALNLGDGSAGIAIDASEA
                     TALRAALVRMAEAHRRTLDQLSSGVAVFDGQRRLAFYNDSYRRLWDLDRAFLDSNPDD
                     SSVLDRLRAARKVPEQPDFRAWKAKLHEAYRAVEPDKDTWYLPDGRAVSVVTTPNPEG
                     GVTYLFDDVTESLDLARRFDGLIRVQRETLDNLAEAVAVFGSNGRAQLFNPAFARMWK
                     LSPEALREQPHIETVESWCRPLLDDAVTWRALREAITAIENRVEVPLKLERKDGSVLD
                     CMTMPLPDGATMLTFQDITDTENVERALRERNEALEAADQMKVDFVHHVSYELRSPLT
                     TIIGFSHFLSDPVTGPL"
     gene            complement(2117..2575)
                     /gene="dut"
                     /locus_tag="VN968_27205"
     CDS             complement(2117..2575)
                     /gene="dut"
                     /locus_tag="VN968_27205"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HXL32954.1"
                     /translation="MSAAVRIDIRQLPHGEGLALPAYQSEHAAGLDLLAAVPEASPMI
                     LSPGQRALVPTGLMIALPSGFEAQIRPRSGLATKHGVTVLNSPGTVDADYRGEIGVLL
                     INHGDAPFPIRRGERIAQMVIASVVRAELVPAAALSATDRGSGGFGSTGR"
     gene            complement(2572..4008)
                     /gene="coaBC"
                     /locus_tag="VN968_27210"
     CDS             complement(2572..4008)
                     /gene="coaBC"
                     /locus_tag="VN968_27210"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HXL32955.1"
                     /translation="MASLTIRRLDDAIRDELRLRAARNGRSVEDEVRVILREASRHRL
                     PAAAPATAEASRVDSDAGAGLPRVTLIMGGGIAAYKALDLIRRLKERHVHVRCVLTRA
                     AQQFVTPLTASALSNERCYTDLFDPESEFDAGHIRLARDCELIVVAPATADLMAKMAQ
                     GHADDLASAILLAANRPILLAPAMNPLMWNNAATRRNVAQLERDGVAMIGPNAGEMAE
                     AGEAGVGRMAEPLEIASTVERLLRPPQPRPLAGKRILITAGPTHEPIDPVRYIANRSS
                     GKQGFAIAIAAQAAGADVTLISGPVDLDDPAGVTVKHVESAREMLHRVEAALPVDIAI
                     FAAAVADWRVANEGEQKLKKTSAGMPPLQLVENPDILAAISKLQDRRPRLVIGFAAET
                     ENLIDNAKAKLARKGCDWIVANDVSPASGVMGGDRNTVHLLTRDDGDTGVDIGIESWP
                     VMTKEQVATELVARIANTGLAKTMEQPS"
     gene            complement(4066..5640)
                     /gene="ubiB"
                     /locus_tag="VN968_27215"
     CDS             complement(4066..5640)
                     /gene="ubiB"
                     /locus_tag="VN968_27215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HXL32956.1"
                     /translation="MISALTHIARLARAGFVFAREGVFGVVDPSLVPPPGQIALRIAR
                     LVERPGAKSGPQLSRALTRLGPAYLKLGQFLATRPDVVGVVMARDLESLQDRLPPFSQ
                     SEAEAVIAASLERPVAQAFASFGPAVAAASIAQVHRGETERDGVRTAVAVKVLRPNVA
                     ARFRRDLADFFFVAHNAEAHSAEARRLRLIEVINTMSRSVAMEMDLRLEAAALSEMAE
                     NTRDDPDFRVPTVDWDRTTHNVLTMEWIDGIALSDHVRLEASQVDLPELGRKVIQSFL
                     RHALRDGFFHADMHPGNLFLDDAGRLVAVDFGIMGRLGTKERRFLAEILLGFITRDYR
                     RVAEVHFEAGYVPGHHSVENFAQAIRAIGEPIHNRTAEEISMAKLLTLLLEVTGLFDM
                     RTRPELILLQKTMVVVEGVARGFDPKLDIWKVADPVVREWIERNLGPIGRIQGAMSGA
                     GELGRVLTGLPAIAARSVAVLEQLEIMTREGLTLSPVTIAAMGRTEGRKSRWRTLALW
                     IIAVTFIGILWAIRQL"
     gene            complement(5637..6398)
                     /gene="ubiE"
                     /locus_tag="VN968_27220"
     CDS             complement(5637..6398)
                     /gene="ubiE"
                     /locus_tag="VN968_27220"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HXL32957.1"
                     /translation="MDRPDQTTHFGFRDVPLGDKQTLVNDVFHSVARRYDLMNDLMSA
                     GLHRVWKNLMINALNPPRSGAPFALLDVAGGTGDIAFRAAEAAGPGFQATVCDINSDM
                     LSVGRGRAAARHLDDRVSFVEGNAEALAFPIRSFDAYTIAFGIRNVPRIDLALSEAFR
                     VLKPGGRFLCLEFSTVDVPGLDRIYDLFSFKVIPPLGRAVTGDADSYQYLVESIRKFP
                     RPGAFAEMMRTAGFARVNWQSFSGGIVALHSGWRL"
     gene            6491..7372
                     /gene="mutM"
                     /locus_tag="VN968_27225"
     CDS             6491..7372
                     /gene="mutM"
                     /locus_tag="VN968_27225"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HXL32958.1"
                     /translation="MPELPEVETVRRGLQPVMEGERIVKAEARRKDLRFPFQKDFVAR
                     LTGQTVTSLGRRAKYLMADLASGDVLLMHLGMSGSFRVITDEDTKTPGKFHHPRAGDR
                     AHDHVVFHMSSGAAIVFNDPRRFGYMKIIARRALDDEPLLSGLGPEPLGNEFDAAMLA
                     RSCANKKTSLKAALLDQRVVAGLGNIYVCEALFRSLLSPRRLAATLATKKAEPTDHAK
                     RLVDAIHAVLNQAIKAGGSSLRDHRQTSGELGYFQHSFQVYDREGEKCQTDGCSGIVK
                     RFTQNGRSTFWCPKCQK"
     gene            complement(7466..8227)
                     /locus_tag="VN968_27230"
     CDS             complement(7466..8227)
                     /locus_tag="VN968_27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXL32959.1"
                     /translation="MLTLVLATQKGGSGKSTLAIGLALAAIQAGHTVRLIETDSQGTL
                     SNWQARRVDAETIVEPVYKAREIEQRLQFLQRSGVTLTIIDTPGGINAATTEAIRYSD
                     FCLIPARPSIADIEAAAPTLHVIRAWKKPFAFVLNQTPIRGHRISNAATAIGDGAAFD
                     VADVVAQPFIVMRNDHQDALSAGLAVSELSPEGKSAEEIRGLWQWIGTKLNSGQQNAL
                     PTFDEQETVIEFPVAPEIDPAIFLRAPARTAALVS"
     gene            8484..10163
                     /gene="ggt"
                     /locus_tag="VN968_27235"
     CDS             8484..10163
                     /gene="ggt"
                     /locus_tag="VN968_27235"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXL32960.1"
                     /translation="MTSNWRDRTDTVFSCRKQPASGSRGMVVSNHPLASAAGAEMLAA
                     GGNAIDAAIATLFTLTVVEPMMVGIIGGGMAHIRLADGSHRFIDGQSTVPLVVRPDTY
                     RSKPGSAHDVFDTVDNENLNGPKAVAVPGSLKAWCETLRRFGTMNLADVMQPAIKHAS
                     RGFAATPYLHECIVDSAKEMRQDKAISAIYLPNGTPLEAGERVVQAEYAGTLATISRD
                     GEAALYQGPLGDILADYMKAHGGFVSREDLASYRTVERSPVRADYRGWEILGPPPPAA
                     SGVHIAQMLNILEGYDIAAMGFGSAATIHHLAEVLKIAFADRAAASGDPDFVGVPVER
                     LTSKAYADERRRTIDVGRAQQWGAGVAQLEGAHTTHMTAADAMGNVVATTQTINNLFG
                     AKFLIPGLGTVPNNYMNLYDPRPGHALSLAPGKRVTTSMSPVMALRDGKLVYALGLPG
                     GKRIFPSALQALLNLIDHGMSLQEAVEAPRVWTEGNALEVELPVPDAVRATLTSMGHN
                     VVAVPTVAGGMNAIQFHENGSLTGAACWRADGTPIGLAGGLARSGVRFGLA"
     gene            10301..>10897
                     /locus_tag="VN968_27240"
     CDS             10301..>10897
                     /locus_tag="VN968_27240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HXL32961.1"
                     /translation="MAFLQLNIRGRLILGFSVLCTLLAGVVGTTIVKVHSVREATDRT
                     VSLRVPTAMTASDLVIGVYASLASLRGWLITGNDAFKAERAGLWKDIQTRGTEMERLS
                     GHWTVEKNRQDWMQAKPLLDELRNAQDKAEAIAHTIDEQPAAKLLATEAAPLAKLMLK
                     NATAIIDEEGNIASTDQRKSLLIGFADMRGSMAMAIGAI"
BASE COUNT         2003 a         3449 c         3502 g         1943 t
ORIGIN      
        1 agcggaccgg tgaccgggtc gctcaggaag tgggaaaaac cgatgatggt ggtcagcggc
       61 gagcgcaact cgtaggacac gtggtggaca aaatcgacct tcatctggtc ggcggcctcg
      121 agcgcctcgt tacgctcgcg cagtgcgcgc tcgacgttct cggtatcggt gatgtcctgg
      181 aatgtcaaca tggtggcgcc gtcgggcaac ggcatggtca tgcagtcgag cacgctgccg
      241 tccttgcgct ccagcttcag cggcacctcg acccggttct cgatcgcggt aatggcctcg
      301 cggagtgcgc gccaggtgac ggcgtcatcg agcaggggcc tgcaccagga ttccacggtc
      361 tcgatatgcg gctgctcgcg cagcgcctcg ggcgacagct tccacatcct ggcgaacgcc
      421 ggattgaaca attgggcgcg gccattgctg ccgaacaccg ccaccgcctc ggcgagatta
      481 tcgagcgttt cacgctggac gcggatcaga ccgtcaaatc gccgcgccag gtccaggctt
      541 tcggtgacgt cgtcgaacag ataggtgacg ccgccctccg gatttggcgt ggtgaccacg
      601 ctcaccgcgc ggccgtcggg cagataccag gtatccttgt ccggttctac cgcgcgatag
      661 gcctcgtgca gcttggcctt ccaggcccgg aagtccggct gttccggaac cttgcgcgcg
      721 gcgcgcagcc gatcgagcac actcgaatcg tcggggttgc tgtcgagaaa ggcgcggtcc
      781 aggtcccaca gccggcgata ggaatcgttg tagaacgcca gccgccgctg gccgtcgaat
      841 accgccacgc cggacgacaa ttgatcgagg gtgcggcgat gcgcttccgc cattcgcacc
      901 agggccgcgc gcagtgccgt cgcctcgctg gcatcgatcg caatgccggc gctgccgtcg
      961 ccgaggttca gcgcatgcac gtcgtagatg cgccgttcgc ccccgatgac gatcggcagc
     1021 cgggccgtaa aggcggcttt ttcgttcagg gcgcggtcca tttcatggcg gtcgccgctg
     1081 tcgagcaatt cgagatcgcg gtcgatcgca tccgcgatgc cggtggcctc ggtcgcccgc
     1141 gcataggcgg cattggcgta gctgaggccg cctttggcgc gtttggccca gatcggccag
     1201 ggtaccgcgg cgacgaaagc gcgcagtatt tcggtttcgc ccagcagggc cttatgccgc
     1261 acgttcaatt cggcgagttc ccggcgcaat ccgctcagcg cacgaatccg cacaacagcc
     1321 tggccaccga tcgcgcggcc catggcctcg acggcatgtc cggcggatgt cgtcagattg
     1381 agcagaaagc cctccccggc gtcgcgcaag gcgtcgaccg catgatcgat ctgcagcgcc
     1441 ggttccggcg gcagccaggt accgaatgca aggatgcgct gcggccgatg cagctgggat
     1501 tcctgcggca tcagtagcga cgtgtcgccg ctgatctgcg ggcgatcgtc gcccgcggcc
     1561 catgaaatca agatctgtgg ttccgcgaac aacaacgcac gtaaacggtc ggcctggact
     1621 tgcagatccc cgatgtcgga gcgcagccgc gcctcgtttc gggtcgcgcg gacgcgggtg
     1681 cgcatcaaga gaaccgccgc caccaccgaa aaaccgagca acgccagagc cgtggccagt
     1741 actgcgatct cctggcggtt gaggtcgagg gataccgaga tcgcatgctg gaacggcaag
     1801 tcgccggcat gagcgggcgc gatcgacatt agggcggctg cgccaaggcc gatcaggccg
     1861 ttgcgcgcca gtgatgtgca cgacagcagg gtccgacgca tcgccacgat cacacccgac
     1921 atggtttgcc ccaaccacac aactttacgc ccggcggttc accccgccgt tcgttcgcga
     1981 atcgagtgac aatatcccct aacgggcttc ggtgggtaag agtccgaacc gtgaacgcga
     2041 accgcagtgc aaaaaatgcg gtattggact tcgatcgacg cgattttcag cgaaatgaca
     2101 ccgcttttta ttcgaactag cgtccggtcg acccgaagcc gccgctgcca cgatcggtcg
     2161 cggaaagcgc cgccgcggga accagttccg cccggaccac ggacgctatc accatctgcg
     2221 caatacgctc gccgcggcgg atcgggaacg gcgcgtcgcc atgattgatc agcagcaccc
     2281 cgatctcccc gcgataatcc gcatcgaccg tgcccggcga gttcagcacg gtgacaccgt
     2341 gcttcgttgc gaggccggag cgcggacgga tctgcgcctc aaagcccgat ggcagcgcaa
     2401 tcataagacc ggtgggtacc agcgcgcgtt gcccggggga aagaatcatg ggcgaagctt
     2461 cgggaactgc cgccaggagg tcgagccccg cggcatgctc gctttgatag gccggcagcg
     2521 ccaggccttc gccgtgcggc aattgccgga tatctattct gacggccgcg ctcacgatgg
     2581 ttgctccatg gttttcgcga gcccggtgtt ggcgatccgt gccaccaatt cagtcgcgac
     2641 ttgctctttg gtcattaccg gccaggattc gattccgatg tcgacgccag tgtctccgtc
     2701 gtcgcgggtc agcagatgca cggtgttgcg gtcgccgccc atcacgcccg aggccggcga
     2761 gacatcattg gcgacgatcc agtcgcagcc cttgcgggcg agctttgcct tcgcgttgtc
     2821 gatcaggttt tcggtctcgg ccgcaaaacc gatcaccagc cgcggccgcc tgtcctgcaa
     2881 tttcgagatc gccgccagga tatccggatt ctccacgagc tgcagcggcg gcatgcccgc
     2941 ggaggtcttt ttcagcttct gctcgccctc attggcgaca cgccagtcgg cgaccgcggc
     3001 ggcgaagatc gcgatatcca ccggaagggc ggcttccacc cgatgcagca tttcgcgcgc
     3061 cgactccaca tgcttcacgg ttaccccggc gggatcgtcg agatcgaccg gccccgagat
     3121 caaggtgacg tccgcgccgg cggcctgtgc ggcaatagcg atcgcaaaac cctgtttgcc
     3181 cgaggagcgg ttggcgatgt aacgcaccgg atcgatcggc tcatgtgtcg ggcctgcggt
     3241 aatgagaatg cgttttccag ccagcgggcg cggttgcggt ggccgcagca ggcgctcgac
     3301 ggtggaggct atttccagcg gttcggccat ccggccgacg ccggcttctc cggcttcggc
     3361 catttcgccg gcattgggtc cgatcatggc gacgccgtcg cgttcgagct gtgcgacatt
     3421 gcggcgggtc gcggcattgt tccacatcag cgggttcatc gctggcgcca gcaggatcgg
     3481 ccggttggcc gccagtagaa tggcgctggc gagatcgtcg gcatgaccct gcgccatctt
     3541 cgccatcagg tcggcggtcg ccggcgccac cacgatcagc tcgcagtcgc gggcgagacg
     3601 gatgtggccg gcatcgaact cgctctcggg gtcgaacaga tccgtgtaac agcgctcgtt
     3661 cgacaaggcg ctggcggtca gcggcgtgac gaactgctgc gcggcgcggg tcagcacgca
     3721 gcggacgtga acgtgtcgtt ctttcagccg gcggatcagg tcgagcgcct tgtaggcggc
     3781 gataccgccg cccatgatca gggtgacgcg cgggaggccg gcgccggcgt cagaatcgac
     3841 gcgtgatgct tctgcggtgg cgggggcagc ggccgggagg cgatgccgcg acgcctcgcg
     3901 caggatcacc cgcacctcgt cttcgaccga gcggccattg cgggccgcgc gcaaccgcag
     3961 ctcgtcccgg atggcgtcgt cgagccttcg gatggtcagg ctggccatgc atgctctcca
     4021 tctcgattcg atatcaatgc tagcagatat atgcaagcat tgcaatcaaa gttgccggat
     4081 agcccagaga atcccgatga aggtcacggc gataatccag agcgccaggg tgcgccaccg
     4141 gctcttgcgg ccctcggtcc ggcccatcgc ggcgatcgtc accggcgaca gggtcagtcc
     4201 ttcccgggtc atgatctcca gttgttccag cacggcgacc gaccgcgcgg cgatcgccgg
     4261 aaggccggtc agcacacggc cgagctcgcc ggcgccggac attgcccctt gaatgcgccc
     4321 gatggggccg aggttgcgct cgatccattc gcgcaccacg ggatcggcga ccttccagat
     4381 gtcgagtttt ggatcgaagc cgcgcgcgac gccttcgacc accaccatgg tcttctgcag
     4441 caggatcagt tcggggcggg tgcgcatgtc aaacaggccg gtgacctcga gcagcagcgt
     4501 caagagcttc gccatcgaga tttcttcggc ggtgcggttg tggatcggct cgccgatggc
     4561 gcggatggct tgcgcgaagt tctcgaccga gtgatgcccg ggcacgtagc cggcctcgaa
     4621 atgcacctcg gcgacgcggc ggtagtcgcg cgtgatgaag cccaaaagaa tttcggcgag
     4681 gaagcgccgc tccttcgtgc cgagccggcc catgatgccg aaatcgaccg ctaccaaccg
     4741 ccctgcgtcg tcgaggaaca gattgccggg atgcatgtcg gcatgaaaga agccgtcgcg
     4801 cagcgcgtga cgcaggaagc tctggatcac cttgcgcccg agttcgggca gatcgacctg
     4861 cgacgcctcg agacgcacgt gatcgctgag cgcaatgccg tcgatccatt ccatggtgag
     4921 gacattgtgc gtggtgcgat cccagtcgac ggtgggcacc cgaaaatccg gatcgtcgcg
     4981 ggtattctcg gccatttcgg acagcgctgc agcctccagc cgcagatcca tctccatcgc
     5041 caccgagcgc gacatcgtat tgatgacctc gatcagccgt agccgccgtg cttccgccga
     5101 atgggcttcg gcgttgtgcg cgacaaagaa gaaatcggcg aggtcgcggc ggaagcgcgc
     5161 ggcgacgttg ggccgcaata ccttcaccgc aaccgcggtg cgcacgccgt cacgctcggt
     5221 ttcgccgcga tgcacctgcg cgatcgaggc tgcggcgacc gccggaccga agctggcaaa
     5281 ggcctgcgcc accgggcgtt ccagcgacgc cgcgatcacg gcttcggctt cgctttgcga
     5341 aaacggcggc agccggtcct gcaggctttc cagatcgcgc gccatcacca cgccgaccac
     5401 atcgggccgg gtggcgagaa actgtccgag cttgagatag gccggtccga gccgcgtcag
     5461 cgcgcgcgac agctgcggcc cggatttggc gccggggcgc tcgaccaatc gcgcgatgcg
     5521 aagcgcgatc tgcccgggcg gcggcaccag cgaaggatcg accacgccga acacgccctc
     5581 gcgcgcgaac acgaaaccgg cccgcgccag tcgcgcgatg tgggtcagtg cagaaatcac
     5641 aaacgccagc ccgaatgcaa cgccacgatg ccgcccgaaa aactctgcca gttgacgcgg
     5701 gcaaagccgg ccgtgcgcat catctcggca aacgcgccgg gcctgggaaa tttccggatc
     5761 gattcgacga gatactgata ggagtcggcg tcgccggtca ccgcccggcc gagcggcggg
     5821 atcaccttga acgaaaacag atcgtagatt cgatcgaggc cgggcacgtc gacggtggag
     5881 aattccaggc acaggaatct tccgccgggt ttcaacaccc gaaacgcttc gctcaaagca
     5941 agatcgatcc gcggcacgtt gcgaatgccg aacgcgatgg tataggcatc gaaactgcgg
     6001 atcgggaacg ccagcgcttc ggcattgcct tcgacaaacg acacccgatc gtcgagatgc
     6061 cgcgccgccg cgcgcccgcg gccgacgctc aacatgtcgg aattgatgtc gcatacggtg
     6121 gcctgaaagc ccgggcctgc cgcctcggcg gcgcgaaatg cgatatcgcc ggtgccgccg
     6181 gcgacatcga gcagcgcaaa cggcgcaccg ctgcgtggcg gattgagcgc gttgatcatc
     6241 aggtttttcc agacccgatg caggcctgcc gacatcagat cgttcatcag gtcgtaacgc
     6301 cgcgccacac tgtggaacac atcgttcacc agcgtctgct tgtcgcccag ggggacatcc
     6361 ctgaagccaa aatgcgtggt ttggtccggc cgatctatca ctttacgccc ctcaacttgc
     6421 agcggccgga ccatagcgcg cccgcggcaa tgtcgctatc acgtcaacat cacaaggtga
     6481 acgcccatcc atgcccgaat tgcccgaagt cgagaccgtc cgccgcggcc tgcagccggt
     6541 gatggagggt gagcggatcg tcaaggccga agccagacga aaagacctgc ggtttccgtt
     6601 ccagaaggat tttgtcgcgc ggctcacggg ccagaccgta accagtctgg gccgtcgcgc
     6661 aaaatacctg atggccgatc ttgcctccgg cgacgtgctg ctgatgcatc tgggcatgtc
     6721 gggctcgttc cgcgtcatca ccgacgagga tacgaaaaca ccgggcaagt tccaccatcc
     6781 gcgcgcaggg gatcgcgcgc atgaccatgt ggtgtttcac atgtcatcgg gtgctgcgat
     6841 tgttttcaac gatccgcgtc gttttggtta catgaagatc attgcccgca gggcgctcga
     6901 tgatgagcct ctgctaagcg gtctcggccc cgaaccgctc ggcaatgaat tcgacgccgc
     6961 catgctggcg cgctcctgtg ccaacaagaa gaccagcctg aaggcggcgc tgctcgatca
     7021 gcgcgtggtc gcgggcctcg gcaacatcta tgtctgcgag gcgctgtttc gttcgcttct
     7081 gtcgccgcga agattggccg cgaccctggc gacaaaaaaa gctgaaccga ccgatcacgc
     7141 caaacgcctg gtcgatgcca ttcacgcggt gctgaatcag gcgatcaagg ccggcggctc
     7201 atcgctgcgc gaccatcgcc agacctccgg cgaactcggc tatttccagc actcgttcca
     7261 ggtttacgac cgcgaaggcg agaaatgcca gactgacggc tgcagcggca tcgtcaagcg
     7321 gtttacccag aacggccgct cgacgttctg gtgtccgaag tgccagaagt gaagtcgcaa
     7381 atgctccttg caacgcaaag ggcgcccctc ttccgaagaa aggggagcgc catttcgtgg
     7441 tcagatcgcc cgcttcaacc cggctctagg aaaccagtgc cgccgtcctc gcgggtgcac
     7501 gcaggaatat cgcgggatcg atttcgggcg ctaccgggaa ttcgatgacg gtctcctgtt
     7561 cgtcgaaggt cggcaaagcg ttctgttggc cgctattcag tttggtcccg atccattgcc
     7621 agaggccgcg aatctcttcg gccgatttac cctccggact gagttcgctc acggccagtc
     7681 cggcgctcag cgcatcctgg tggtcgttac gcatcacgat gaacggttgc gcgacaacat
     7741 cggcaacgtc gaacgccgct ccgtcgccaa tcgcggtcgc cgcgttactg attcggtggc
     7801 cgcgaatcgg cgtctggttg aggacaaaag cgaatggctt cttccaggcg cggatcacat
     7861 gaagcgtcgg tgcggcggct tcgatatcgg cgatgctcgg gcgcgccggg atcaggcaga
     7921 agtcggaata gcgaatcgct tcggtcgtgg cggcgttgat gccgcccggg gtgtcgatga
     7981 tcgtcaacgt tacgccactg cgttgcagaa actgcagccg ctgctcgatc tcgcgggcct
     8041 tgtaaaccgg ctcgacgatg gtctcggcat cgacgcggcg cgcctgccag ttcgaaagcg
     8101 tgccttgaga atcggtctcg atcaggcgaa cggtgtgccc ggcttgaata gccgcaaggg
     8161 cgaggccgat ggcaagcgta ctcttgccgc tgccaccctt ttgggtggcc aatacgagcg
     8221 taagcattga agattccttg gaatgttttt acggagatct gcggttacgc cgaactgagt
     8281 tgcatcgtgt tacgatgccg agcgcttctc gctcaggacg tgccggcccg atccaaggct
     8341 cacaggatcg cggctgtccg aatcatcacg tgcaatgatg gcttactttg gggaatatgg
     8401 ctattccggg gtaatactga cgcgagccga ccatgcaata aagtgtcagc cgtggcactc
     8461 ctgcaacaac acagggtgga aaaatgacca gcaactggcg ggatcgcacg gacacggttt
     8521 tcagttgccg gaagcagccg gcgtcgggca gtcgcggcat ggtggtcagc aaccatccgc
     8581 tggcgtcggc cgcgggcgcg gagatgcttg ccgccggcgg caacgccatt gatgcggcga
     8641 tcgcaaccct gttcacgctg accgtcgtcg agccgatgat ggtcgggatc atcggcggcg
     8701 gcatggcgca tatccgcctt gccgacggca gccaccgctt catcgacggc cagagcacgg
     8761 ttccgcttgt ggtcaggccg gacacctatc gatcgaagcc gggttcggca cacgatgtat
     8821 tcgataccgt cgataatgaa aatctgaacg gtccgaaggc ggtagccgtg ccgggctcac
     8881 ttaaggcctg gtgcgaaacc ttgcgccggt tcggcaccat gaatctcgcc gacgtcatgc
     8941 agccggcgat caaacatgcg tcgcgcggtt ttgcggcgac gccctatctg cacgaatgca
     9001 tcgtcgatag cgcaaaggag atgcgccagg acaaggcgat ctcggcgatc tacctgccga
     9061 acgggacgcc gctggaggcc ggcgagcgcg tggtacaggc cgaatatgcc gggacgctcg
     9121 ccaccatctc gcgcgacggc gaagcggcgc tgtaccaggg gccgctcggc gacatcctgg
     9181 cggattacat gaaggcgcat ggcgggtttg tcagccgcga agacctcgcg tcctaccgga
     9241 ccgtcgaacg ttcgccggtg cgagccgatt atcgcggctg ggaaattctc ggtccgccgc
     9301 cgcccgcggc ctccggcgtg cacatcgcgc agatgctgaa tatcctggag ggctatgaca
     9361 tcgccgccat gggattcggc tcagccgcga ccatccatca tctcgccgaa gtgttgaaga
     9421 tcgcctttgc cgatcgcgcc gccgcgagcg gcgatcccga ttttgtcggt gtgccggtgg
     9481 agcggctaac ctcgaaggcc tatgccgatg agcgccgccg cacgatcgat gtcggacgcg
     9541 cgcaacagtg gggtgcgggt gtggcgcagc tcgaaggcgc gcacaccacg cacatgaccg
     9601 ccgcggatgc gatgggcaac gtggtcgcga ccacgcagac catcaataac ctgttcggcg
     9661 cgaagtttct gattccgggc ctcggcaccg tgccgaacaa ctacatgaat ctctacgatc
     9721 cccggcccgg acacgccttg tcgctggcgc cgggcaagcg cgtcaccacc tcgatgtcgc
     9781 ctgtcatggc gctgcgcgat ggcaagctgg tttatgcgct cgggttgccg ggcggaaaga
     9841 ggatttttcc aagcgcgttg caggcactgc taaacctgat cgatcacggc atgagcttgc
     9901 aggaggcggt cgaggcgccg cgggtctgga ccgagggcaa tgcgctcgag gtcgaattgc
     9961 cggttccgga tgcggtgcgc gccacgctga cttcgatggg tcacaacgtc gttgcggtgc
    10021 cgaccgtggc cgggggcatg aacgccatcc agttccacga gaacggctcg ctcacgggcg
    10081 cagcctgctg gcgtgccgac ggcacgccga tcggccttgc cggagggctg gcgcgttcgg
    10141 gggttcgatt tgggctggcg tgagaggctc tgcgaaccgg acgagcgctc tatggatcct
    10201 gtattagtac cgatcagtca ttgttccaat cttcatggtt aactgacagc ctatcctttg
    10261 agcttgccta ggcggcggct tgcatcgaag ggacttcacc gtggcattcc tgcaactgaa
    10321 tattcgtggg cgattgattc ttggctttag cgtgctttgc actctcctgg ccggtgttgt
    10381 cggcaccaca atcgtcaaag tgcactcagt gagggaggcg accgatcgca ccgtcagctt
    10441 gcgggttccg accgccatga cggccagcga tctggtgata ggagtatatg cttcgcttgc
    10501 atcgctgcgg ggctggctga tcaccgggaa cgacgcattc aaggcggagc gcgccggcct
    10561 ttggaaagac atccagacac gcggcaccga gatggagcgt ctctcgggcc actggaccgt
    10621 cgaaaaaaac aggcaggact ggatgcaggc caagccgttg ctcgacgaat tacgcaacgc
    10681 gcaagacaag gccgaggcga ttgcccatac tatcgacgag caacccgcgg caaagctgct
    10741 tgcgaccgag gcagcgcctc tcgccaaact catgctcaaa aacgctaccg ccatcatcga
    10801 cgaggaaggc aacattgcct cgaccgatca gcgcaagagc ttgttgattg gctttgccga
    10861 tatgcgaggc agcatggcaa tggcgatcgg cgccatc
//
LOCUS       DATNOI010000679         5243 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1217893,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000679 DATNOI010000000
VERSION     DATNOI010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217893"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /gene="ispH"
                     /locus_tag="VN968_27245"
                     /gene_synonym="lytB"
     CDS             complement(<1..276)
                     /gene="ispH"
                     /locus_tag="VN968_27245"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491532.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HXL32962.1"
                     /translation="MQAKKPLKIVLCSPRGFCAGVVRAIDTVERALTIYGAPVYVRHE
                     IVHNRYVVDSLKTKGAIFVEELAEIPDNTDAPVVFSAHGVPKSVPADA"
     gene            494..1186
                     /locus_tag="VN968_27250"
     CDS             494..1186
                     /locus_tag="VN968_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HXL32963.1"
                     /translation="MSNAPLMPKATAVWLVDNTALTFDQVADFTKMHPLEVRAIADGD
                     AAQGIKGMDPISTGQLTRDEIEKGEKDPNYRLKLGESKVTLPPQAKKKGPRYTPVSRR
                     HERPSAILWLVRNHPELKDAQIMRLVGTTKTTIASVRDRTHWNASTLTPMDPVTLGLC
                     SQIELDFEVQRAAKEKPADTAYGGATLLPASETTRKEPEFEPTEKQRDDLNVDAVFAK
                     LKTIGGKKAEEE"
     gene            complement(1491..2888)
                     /locus_tag="VN968_27255"
     CDS             complement(1491..2888)
                     /locus_tag="VN968_27255"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HXL32964.1"
                     /translation="MVPEMRKTGIDVVGDMAWGTHFCLFYETKRDLLDTMISYCKTGL
                     ESEEFCLWVVAEPLTIEEAMDALKDAMPDLDRYLADPSIEIVSARDWYLQDGTFDLKR
                     VTGRWHEKLAHASARGYAGVRVTGDTAWLEKKDWKDFCEYEEGLNEAIANRHLAVLCT
                     YPIATCGAVEVLDVTRTHQFALARRHGSWDVIETAGHKQAKAEIKRLNEELEQRVVER
                     TSQLMLASEALREAQTELAHVNRVTTMGQLAASIAHEVNQPIAGVVTNADAALRWLGH
                     RPPDLEKVQYSLDEIIKDGNRAGDVIGRIRALIKNVPPRKDRLEINEAILEIIALTHG
                     EAVKNSVSLQTQLAAGLPLIQGDRVQLQQVILNLIVNAVEAMSGVSEGSRELLISTGK
                     DSSGVLVAVQDSGPGLNPKSFDRLFDAFYTTKPGGMGMGLSICRSIVEAHGGRIWASR
                     TAGPGATVQFTLPCG"
     gene            complement(3222..3428)
                     /locus_tag="VN968_27260"
     CDS             complement(3222..3428)
                     /locus_tag="VN968_27260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HXL32965.1"
                     /translation="MNEDVFNASLRKFLKKVGITSQREIEKAVRDALESGRLKGNEKL
                     PAKIVLTVGGVNLAHEVKDEIELG"
     gene            complement(3726..3995)
                     /locus_tag="VN968_27265"
     CDS             complement(3726..3995)
                     /locus_tag="VN968_27265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32966.1"
                     /translation="MRRNSAQLLIALILATPLLAGCLERGKPTIVDTSGDDDAICRAN
                     NVAAGSSEYVACRRDRDVVRNNATARADRRQRDLGEYMLNHPDRP"
     gene            complement(4256..>5243)
                     /locus_tag="VN968_27270"
     CDS             complement(4256..>5243)
                     /locus_tag="VN968_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXL32967.1"
                     /translation="GATSIMYEGKPVGTPDAGAFWRLISEHKAVALFTAPTAFRAIKK
                     EDPDGKFIRQYDLSKFRTLFLAGERADPPTVEWAEAQLKVPVIDHWWQTETGWCIAGN
                     PMGLGMLPVKHGSPTVPMPGYQVDVVDEAAKPLPAGTMGSIVVKLPMPPGCLPTLWEQ
                     DERFKEAYLTEFPGYYKTSDAGYKDEDGYVWVMGRTDDIINVAGHRLSTGGMEEILAS
                     HPDVAECAVLGVRDAIKGEVPCGFVVLKAGVTKSHADVEKEIVALVRDKLGPVAAFKL
                     AITVARLPKTRSGKILRGTIKKIADGEPWAMPATIEDPKVLDEIGAALKGRV"
BASE COUNT          968 a         1640 c         1582 g         1053 t
ORIGIN      
        1 ggcgtcggcc ggcacggatt ttggaacccc gtgggcggaa aacaccaccg gtgcatcggt
       61 attgtcgggg atttcagcca gttcctcgac gaaaatcgcg ccttttgtct tcaggctgtc
      121 gacgacgtag cggttatgga cgatctcgtg gcggacatag accggggcgc catagatggt
      181 gagcgcccgc tccacggtat cgatcgcccg caccaccccg gcgcagaagc cccgcggcga
      241 gcaaagcacg attttcaggg gtttttttgc ctgcatggga agggtctcgg accgaatcac
      301 ccctcgccgc cgggcaggga acggtcaatt cggctaagca cttgggactg gtttcgggcg
      361 gtgtcaagac cgattagcag cccattgcgt gggcagcccc cgacggccta tatagggccg
      421 taattcccgt catcgctgat gaccacggct tcgccttcaa aaagaggtgg gcgaagcgca
      481 aaggagattt gccatgagca atgcaccgct gatgccaaaa gcgacggccg tgtggcttgt
      541 cgacaatacc gcgctgacct tcgatcaggt ggcggatttc accaagatgc atccgctcga
      601 ggttcgtgcc atcgccgacg gcgacgccgc ccagggcatc aagggcatgg acccgatttc
      661 gaccggtcaa ttgacccgcg acgagatcga aaagggcgag aaggacccga actaccggct
      721 caagctcggc gagagcaagg tgacgctgcc gccgcaggcc aagaagaagg gcccgcgcta
      781 cacgccggtg tcgcgccgcc atgagcggcc gagcgcgatc ctgtggctgg tgcgcaacca
      841 tcccgaactg aaggacgcgc agatcatgcg gctggttggc accaccaaga ccacgattgc
      901 cagcgtgcgc gaccgcaccc actggaacgc ctcgacgctg acgccgatgg acccggtgac
      961 gctcggcctg tgctcgcaga tcgaactcga tttcgaggtg cagcgcgcgg ccaaggagaa
     1021 gccggccgac accgcttacg gcggggccac gctgctgcca gcctccgaga ccacccggaa
     1081 agagccggaa ttcgagccga ccgaaaagca gcgcgacgat ctcaatgtcg atgccgtgtt
     1141 cgccaagctg aagaccatcg gcgggaagaa ggcggaagag gagtaggcgc cactcggctg
     1201 tcatctctca aagtcgtccc cgcctagtgc gcaattgcgc acggggagcg gggacccatc
     1261 acgccgcggc ggcgcgcttt ggcacgatga ccgacggctt gtaactccaa cagacggtgg
     1321 tggttatggg tcctcgcgtt cgcgaggacg acacacagca tgtggcgcgc tcgggctcag
     1381 cgcatcgctt cggacaaatt ttcaaacagc gttattctcc gacacagcac cgcgactacc
     1441 gttcacgatg ccgggggtga cgactagcgt agtttcacgt ttgcacactc tcacccacag
     1501 ggcagggtga actgaacggt cgcgcccggg ccggcggtgc gagaggccca tatccgtccc
     1561 ccgtgagctt cgacgatcga acggcagatc gatagtccca tgcccatgcc gccgggcttg
     1621 gtcgtgtaaa atgcgtcgaa caggcggtca aagctcttcg ggttcagccc cgggccggaa
     1681 tcctgcaccg cgacgagcac cccactcgaa tctttcccgg tgctgatcag caactctcgc
     1741 gacccctcgc tgacgccgct catcgcttcg acggcgttga cgatcaggtt aaggatcact
     1801 tgttgcagtt ggactcgatc tccttgaatg agcggcaagc cggccgcgag ttgcgtctgc
     1861 aacgagacgc tgttcttcac cgcttcgcca tgggtcaggg caatgatctc gaggatcgct
     1921 tcgttgatct ccaggcgatc cttccgcggg ggcacattct tgatgagggc gcgtatccgg
     1981 ccgataacgt cgccggctcg attgccgtcc ttaatgatct catcgaggga gtactgaact
     2041 ttctccagat cgggaggccg gtggccaagc cagcgcaaag ccgcatcggc gttggtcacc
     2101 acgccggcga tcggctggtt gacttcatgc gcaatcgagg ccgcaagctg ccccatcgtc
     2161 gttacgcgat tgacatgcgc gagctccgtc tgcgcctcgc gcaacgcttc cgaggccagc
     2221 atcagttggc tggtccgttc cacgacacgt tgttcgagct cctcattgag ccgcttgatt
     2281 tcggccttgg cctgcttgtg cccggcggtt tcgatgacgt cccaactccc gtgccgcctt
     2341 gccagggcaa actggtgtgt acgcgtcaca tccaggactt caacagctcc gcaggtcgca
     2401 atggggtacg tgcacagcac ggccaggtgc cgattggcga tagcctcgtt gagaccctct
     2461 tcgtattcgc agaagtcttt ccagtccttc ttttccaacc aggccgtatc cccggtcact
     2521 ctcacgccgg cgtaacctct ggccgatgcg tgagcgagtt tctcatgcca acggcctgtt
     2581 accctcttga gatcgaacgt gccatcctgc agataccagt cgcgggccga gacaatttca
     2641 atgctgggat ccgccaggta tcgatcaagg tcaggcatcg cgtctttcag cgcatccatg
     2701 gcttcttcga ttgtcaaggg ctcggcaaca acccacaggc agaactcctc gctttccaat
     2761 ccagtcttgc agtatgagat catcgtgtcg aggagatccc tttttgtctc atagaagaga
     2821 caaaaatggg taccccaggc catatcgcca acgacgtcga ttccggtctt ccgcatctcg
     2881 ggtaccatcg cggtgtcccc aatcttcagc ttgggatcgt tgccggcacg aaccggatag
     2941 gcggattccc ctcatccggc tctcggacaa aacttcacgc cttcaactac ggcacgtagt
     3001 atccaggccg gctcacgcat cgtctgctgc gagtgtgtgg agagacacaa cgtctgctgc
     3061 tgaccctccg cgaatggaat cggaattccg tccgcgggag tgttccttag acgacctgat
     3121 ctctatcgtt caacatggag tagcttacca aagacggcgt ttttgtccac cgacgacggt
     3181 ggcagatgag gcgccgaacc cgccgatttg cgcgccgcgg cctaccccag ttcgatctcg
     3241 tccttgacct cgtgggcgag gttgacgccg ccgacggtga gaacgatttt ggccggcagt
     3301 ttttcgttgc ccttgaggcg gccggattcc agcgcgtcgc gcaccgcctt ttcgatttcg
     3361 cgctgcgagg tgatgccgac ctttttcaga aatttgcgca ggctggcgtt gaagacgtct
     3421 tcattcatga tgacctccgg attgcgcgtc gatcatacac tggtaacggc gtggcacgaa
     3481 accttatcct ttgaggatgc gctcgggacg aagcacggcg ttccatcctc gtcattgcga
     3541 ggagcaaagc gacgaagcaa tccattcttc gtcattccgg gattgtgcgc cagcaccaga
     3601 cctcagatgt gcaattgcac atcggggaat ctcgagattc cgggctcgat gcttcgcatc
     3661 gccccggaat gacggagcca tggattgctt cgtcgctttg ctcctcgcaa tgacgggtta
     3721 tccagttacg ggcggtcggg gtggttcagc atgtattcgc cgagatcgcg ctggcggcgg
     3781 tcggcgcgcg cggtggcgtt gttgcgcacg acgtcgcgat ccctgcggca ggcgacatat
     3841 tcgctcgagc cggcggcgac attgttggcg cggcagatcg catcgtcgtc gccgcttgta
     3901 tcgacgatgg ttggctttcc ccgctcaagg cagcctgcaa gcagcggggt ggcgaggatg
     3961 agcgcgatca gcaattgtgc ggagttgcgt cgcatgcgcc gatttccatg gttgtttcga
     4021 tttgccgggc ttgacccggc aatccatcga aataaaggca atttctgatc gcggaggagt
     4081 aagcccctcc gtcgtcattg cgaggagcgc tggcgacgaa gcaatccatt ctttctttgc
     4141 gtggagaaat ggattgcttc gcggagcctg tcatcgggcg cgcgttcgcg cgacccgttg
     4201 gctcgcaatg acgacaccat gtgaccagcg cttgggtgag gccaacagac ttccctcaca
     4261 ccctgccctt cagcgcggcg ccgatttcgt ccagcacttt tggatcctcg atggtggcgg
     4321 gcatcgccca gggctcgccg tcggcgatct tcttgatggt gccgcgcagg atcttgcccg
     4381 aacgcgtctt cggcagccgc gccaccgtga tcgcaagttt gaacgccgcc acgggaccga
     4441 gcttgtcgcg caccagcgcc acgatctcct tttcgacatc ggcatggctt ttggtcacgc
     4501 cggccttcag caccacgaag ccgcagggca cctcgccctt gatcgcgtcc ctgacgccga
     4561 gcaccgcgca ttcggcgacg tcgggatgcg aagccagaat ctcctccatg ccgccggtgg
     4621 agagccggtg gccggcgacg ttgatgatat cgtcggtgcg gcccatcacc cagacatagc
     4681 catcctcgtc cttgtagccg gcgtcggagg ttttgtagta gccggggaat tcggtgaggt
     4741 acgcttcctt gaagcgctcg tcctgctccc agagcgtcgg cagacagccc ggcggcatcg
     4801 gcagttttac gacgatcgag cccatggtgc ccgcgggcaa cggctttgcg gcttcatcga
     4861 ccacatcgac ctgatagccc ggcatcggca ccgtcggcga gccgtgcttc accggcagca
     4921 tgcccaagcc catcggattg ccggcgatgc accagccggt ttcggtctgc caccagtgat
     4981 cgatcaccgg caccttcaat tgcgcttcgg cccattccac cgtcggcgga tcggcgcgct
     5041 cgccggccag aaacagcgtg cggaattttg aaaggtcgta ttgcctgatg aacttgccgt
     5101 ccgggtcttc cttcttgatg gcgcggaatg cggtcggcgc ggtgaacagc gccaccgcct
     5161 tgtgttcgga gatcagccgc cagaacgcgc cggcgtcggg cgtgccgacc ggcttgccct
     5221 catacatgat cgaggtcgcg cca
//
LOCUS       DATNOI010000680         2360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1577377,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000680 DATNOI010000000
VERSION     DATNOI010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577377"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VN968_27275"
     CDS             <1..665
                     /locus_tag="VN968_27275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32968.1"
                     /translation="RARPGSVIVRHYRDAEINAFERAVARDIGPGAVIVIPDDAFVEE
                     ARRILPVRVLAQNWVQLYHASIEASLPEIRGDTLSAKARTVLAQIQARGARTESHGAV
                     LDWLRVEEHKSTPRDQLRPHAPQRRREFNAFMAIINVTEGIAEKIWTEGIEPLRIDRR
                     RAGLRMAQAFVSVLIDPHAAAAGLEASVRESIGTLRRRALDHLDEVVGRETYGAREGQ
                     VA"
     gene            908..1843
                     /locus_tag="VN968_27280"
     CDS             908..1843
                     /locus_tag="VN968_27280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015498222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HXL32969.1"
                     /translation="MASRWKTDRPMSRAEFDARFPDRTACARYLAAKRWPDGFVCPAC
                     GVCKGWELETKPFTWECSGCHRQTSVTAGTVMHRSKLPLRTWFEAIHQLASHSNGISA
                     EQAQAQFGIGSYKTAWLLLHKLRRAMVSPERSLLQAMVEADETEVPLRSKHDPIKGYG
                     IPNVGKLLIIGAVELSPDGHPRRVRLEPLRDRTGENVRGFVERVVAPDATVVSDGLAS
                     YLKLGEHRHVGKTVGSMAAHILLPWVHRVFANLKRWLTGTFHGARKQHLKRYLDEFTF
                     RWNRRRHTRTAFDNLLGLVTRLPHASLRDFVDQRV"
     gene            1891..2133
                     /locus_tag="VN968_27285"
     CDS             1891..2133
                     /locus_tag="VN968_27285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32970.1"
                     /translation="MVDRIAHAIAEADGGDFQADPARYRRLALAALQPLASPTEAMID
                     AAHQAVSFDDAWAINNRSDFKRSVKAMLAVSAVKRE"
     gene            complement(2178..>2360)
                     /locus_tag="VN968_27290"
     CDS             complement(2178..>2360)
                     /locus_tag="VN968_27290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32971.1"
                     /translation="FTLMRIVSLDIAGDEITHSDISEDGKQITHRDETSEYFYNAHDL
                     NTARKAPPVGSGAATG"
BASE COUNT          446 a          689 c          783 g          442 t
ORIGIN      
        1 tgcgggcacg tccgggatcg gtcatcgtcc gccactatcg tgacgctgag atcaatgcct
       61 tcgaacgcgc ggtggctaga gatattggtc ccggtgccgt aatcgtcatt cctgacgatg
      121 cattcgtgga agaagctcgg cgtatccttc ctgtgcgtgt gctcgctcaa aactgggtgc
      181 agctttacca cgcatcgatc gaggccagcc tgccggaaat tcggggcgat acacttagtg
      241 ccaaagcgcg gactgtactg gcccagatac aggcgcgcgg cgctaggaca gaaagtcacg
      301 gggctgtcct cgattggctg agggtggaag agcataaatc cacacctcgc gatcagcttc
      361 gaccgcatgc gccccagagg cggcgggagt tcaatgcatt catggcgatc attaacgtga
      421 ccgaggggat tgcggagaag atctggactg aaggtatcga gccgctacgc atcgatcgcc
      481 ggcgcgccgg cctccgtatg gcgcaggcat tcgtttccgt tctgattgac ccgcacgccg
      541 ccgcggccgg gttggaggcg tccgtacgtg agagcatagg gactctccgg agaagagcgt
      601 tggaccatct cgatgaagtg gtcgggcgcg aaacctacgg tgccagggag ggacaggtgg
      661 catgagcgag tcagagcttc gaacggcagg aagggtctcc cgtgcagtcg atctcgaggc
      721 tcttgaacga ggaatgcgtg ccgatcaagg ggcggatttg actgaagcga aggaggcgtt
      781 cgttgacgca ttgcttggat tgacagtgcg acgaaggctc ctcccgttgg ttccggcgcc
      841 cccagtggta gtagctgggc ctgcgggaat cggttgacag ccggccaaat gttgccatta
      901 tgttctaatg gcgagcagat ggaaaaccga tcggccgatg tcgcgggcgg agttcgacgc
      961 tcggtttccg gaccggacgg cgtgcgcccg ctatctcgcc gcgaagcggt ggccggatgg
     1021 tttcgtctgt cctgcctgcg gcgtttgcaa gggctgggag ctcgagacca agccgttcac
     1081 ctgggagtgt tccggctgcc accggcagac gtcggtgacc gccggtacgg tgatgcatcg
     1141 cagcaagttg ccgctgcgga cgtggttcga ggcgatccac cagctggcga gccattcgaa
     1201 cggaatctcc gccgagcagg cgcaggcgca attcggcatc ggcagctaca agacggcttg
     1261 gctgctgctg cacaagctgc gccgggcgat ggtatcgccg gagcgcagtt tgctgcaggc
     1321 catggtggag gcggacgaga ccgaagtgcc gttgcgctcg aagcacgacc cgatcaaggg
     1381 ttatggtatc ccgaatgtcg gcaagctgct gatcattggc gcggtagaac tctccccgga
     1441 cggtcacccg cggcgggtgc ggctggagcc gttgcgagac cgcaccggcg agaacgtccg
     1501 cggctttgtt gagcgcgtgg tggcgcctga cgcgacggtg gtcagcgacg gtctggcgag
     1561 ctacctaaag ctcggggaac accggcacgt gggcaagacc gtgggcagca tggcggcaca
     1621 catcctgctg ccctgggtgc atcgggtgtt cgccaatctg aagcgctggc tcaccggcac
     1681 gttccatggc gcgcgcaaac agcacctcaa gcgctatctc gacgaattca ccttccggtg
     1741 gaaccgccgg cggcataccc ggacggcgtt cgacaacctg ctcggcctgg tcacccgcct
     1801 gccgcacgcc tccttgcgag acttcgtcga ccagcgggtc tgaacagcgc ttgccaccca
     1861 atgccgcgac gaacccttca cgccgtgaac atggtcgacc ggatcgccca cgccatcgcc
     1921 gaggccgatg gcggcgactt ccaggctgat cctgccaggt atcggcgact ggccctggca
     1981 gcgctgcagc cgctggcgag ccctacagag gcgatgatcg acgcggcgca ccaagcggtc
     2041 tcgttcgatg acgcctgggc catcaacaac cgcagcgact tcaagcgctc ggtgaaggcg
     2101 atgctcgccg tctcggcggt caaaagggaa tgatcaaaac cggtaggcgt agcggcgtgg
     2161 acgacagtgc ggcgctatca acctgttgcg gcaccggaac caacgggagg agccttccgc
     2221 gctgtattta ggtcatgggc gttgtagaaa tattccgatg tctcgtcgcg atgtgttatc
     2281 tgctttccat cctcacttat gtcggagtgg gttatttcat cgccagcaat atctagtgag
     2341 acaatcctca taagtgtgaa
//
LOCUS       DATNOI010000681        12460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2344482,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000681 DATNOI010000000
VERSION     DATNOI010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 12460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12460
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2344482"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            583..765
                     /locus_tag="VN968_27295"
     CDS             583..765
                     /locus_tag="VN968_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32972.1"
                     /translation="MMKIVMIAGATLALVTAAGAADLPHPQPVYQTAQLGKMPIGKAP
                     IGKSPIGKAPVVTRSY"
     gene            831..1538
                     /locus_tag="VN968_27300"
     CDS             831..1538
                     /locus_tag="VN968_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32973.1"
                     /translation="MANRPKRLGRCLLAGFTLAVLLQCKIQSAAAHEIDTRAGSERFI
                     SQKREVQKRTADANPLVSSEAPIESKPAPRSTRKSGSGSAKGPYYVDFRARTAASWGH
                     AFVWFGKTSEREVEVAGLTPAGDNLAYVLGHVTWVPSETGASYGDLDPQYLTASYRVY
                     LNEPDAKRVFAYIKKLQTSSPVWNAETSNCTSFIGSIANFMGLKVPLRWQRPEDYVNS
                     LKTMNGGSQIVHLSSEQ"
     gene            1851..3404
                     /locus_tag="VN968_27305"
     CDS             1851..3404
                     /locus_tag="VN968_27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXL32974.1"
                     /translation="MPVRHYDWIAHFARRTPDKLAVVDLASERRLTYAQFDARISHLA
                     THLRDGVKIARGDRVAVLAHNTTDTLEVQFACGRIGAVFLPLNTRLTVPELSYIVGDA
                     APQLMIHDAALADLALAVAKLCEVPSALLLGAGGSFEAAIAAAKPLDQAEIVTLDDMS
                     TIMYTSGTTGQPKGAIITHGMTFWNCVNLGGPAYISPSSVLLTVLPLFHTGGLNCYTN
                     PVLHAGGTVLIMRAFDPGLALQLISDPSHRINTFFGVPSIYQFMAQHPAFAGSDFSRL
                     VICGVGGAPMPVPLLKVWEERGVALQQGYGMTETSPAVLTLDREDAVRKAGSSGKPVL
                     HTEVRIVRPDGVDADIGELGELWVKGPNITPGYWNRPDANQSSFTDGWLHTGDAARID
                     EEGFYYIVDRWKDMYISGGENVYPAEVENVLHQLAAIAEAAVIGIADAQWGEVGMAIV
                     AVKPGHSLTEAEIQAHCEANLARFKRPCVIRFVEALPRNATGKIHKPTLRQNFGIPTA
                     TDSANAVAS"
     gene            3465..4709
                     /locus_tag="VN968_27310"
     CDS             3465..4709
                     /locus_tag="VN968_27310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL32975.1"
                     /translation="MNNKKRSLLASHLIAVAAATALSVLATQGALAQKKYDTGATDTE
                     IKIGNVEAYSGPASAYGIIGKTEDAYFRMINEQGGINGRKINFISYDDGYSPPKTVEQ
                     VRKLIESDEVFLVFNALGTPTQNAVQKYHNAKKVPQLFLATGASKWNDPKDYPWTMGF
                     QPSYRVEARIFAKYIVKTKPDAKVAVFYANDDFGKDYVAGLKEIFGDKSPSILVAEES
                     YETTEPSIDSHIVKLKGTGADVFVNISTPKFAAQAIKKMAELEWKPMHVMTDVSISIG
                     AVMKPAGLEASEGILSAGYLKDASDPQWKDDEGMKKFMTFIDKYSPGANISDANLVYA
                     YAAAQTMVQVLQQCGDNLTRENVMKQAASLKDFAPDTLLPGIKIGTSATDFAPIEQLK
                     MMQFKGGKWDLFGDIISAETGG"
     gene            complement(4913..6565)
                     /gene="pgm"
                     /locus_tag="VN968_27315"
     CDS             complement(4913..6565)
                     /gene="pgm"
                     /locus_tag="VN968_27315"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HXL32976.1"
                     /translation="MAVEVNPLAGKPAPASMLVNVPRLVTAYFSNKPDPKIPSQRVAF
                     GTSGHRGSAFNNAFNEAHILAISQALCDHRRQSGIDGPLFIGIDTHALAECAFASALE
                     VFAANDVTVMIDERDGYTPTPAISHAILTYNKNRTTGLADGVVITPSHNPPEDGGFKY
                     NPPNGGPADADVTTGIERAANAFLEDGLRGLHRISYHRAHKARCVHRYDYIGPYVADL
                     ANVIDMEAIRASGVKIGIDPLGGAAVHFWQPIIERYGIAATIVSDTVDPTFRFMTADW
                     DGKIRMDCSSPYAMARLIGMRDKFDVAFANDTDADRHGIVTRSNGLMNPNHYLAAAIA
                     YLFENRPGWSKDSAVGKTIVSSAIIDRVAAKLGRRLVETPVGFKWFVDGLIGGSFGFA
                     GEESAGASFVRRDGSVWTTDKDGIILGLLAAEITARTKQDPSQLFDRLTGELGVPFYE
                     RIDAPATVAQKNLLKALSPEQLAIKELAGEPLRATLNAAPGNNQSFGGIKVVAENGWF
                     AARPSGTEDVYKIYAESFISQQHLRQIQQQAQAAITASFEAG"
     gene            complement(6596..7360)
                     /locus_tag="VN968_27320"
     CDS             complement(6596..7360)
                     /locus_tag="VN968_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL32977.1"
                     /translation="MLTPDKQLSVEQSRQVAETIREEIARRRISRQSLAEQAKLSLST
                     LEKVLGGRRPFTLATTVRLEQALGVSLRKAQDSAPAAVPVNGDVSPDGLGAYSRRSVE
                     WLEGVYVTVRPSFGEKDAIYAYRTEIAWDPAVSSLVFREGERQDAAYTQFGEVAVPNQ
                     SGHIYLITNRHGQHRLIMASLPRKAGEMYGIITTLLAGRGSLLTPIAAPIALLRIEAF
                     LSPAFGRISPGDASYASYREHLRRIAEEPFAVFLPG"
     gene            7546..9822
                     /locus_tag="VN968_27325"
     CDS             7546..9822
                     /locus_tag="VN968_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HXL32978.1"
                     /translation="MTTCDEIETAESSGLWRIVRLLLFCVMQGAAVILVGFAALFLSF
                     EPVWSAENPSATLASLSDARSGSLLLKTDDGYADATRLGIDVDLTVSGPTVRARVTQI
                     FRNPTKNWVEATYVYPLPAGGAVDTLKMVIGDRVVVGDIKERQQARIIYEQAQRNGQK
                     AALTEQERPNIFTNSVANIGPGETVLVQIEYQEPVQQMGNEFSLRVPMVVGPRYNPAP
                     VVQSVDFRPNGGGWGVTSADPVPDRNRISPPVLDPAEHGPVNPTAITVRLQAGFPLGE
                     VKSHHHQVKIDSPDDSTRVIRLAEGVVPADRDFELSWKPAAEKAPSVGLFREHVGDAD
                     YLLAFVTPPSVEAAEQKPLPREVIFVIDNSGSMGGTSIIQAKASLTYALGRLQPNDRF
                     NVIRFDHTMDVLFPHPVPADAEHVASAKSFVGALQAEGGTEMVPAMQAALTDNAAGDA
                     NTVRQVVFLTDGAIGNEQQLFDTITAMRGRSRVFMVGIGSAPNTFLMTRAAELGRGAF
                     THIGSVEQVEERMRGLFAKLENPAVTGLAAKFSEATADLAPAAIPDLYRDEPLVLAAK
                     LDTLKGSVEIKGRIGDRPWVVTLALANAAEGKGLSKLWARRKIDDAEIARTLRTATPE
                     DADKTILALALQHQLVSRLTSLVAVDETPSRPEGEALKLSELPLNLPAGWDFAKVFGE
                     RPRLPSPPAERRADAGDARVHLAAAKRMPLAVTPAPGTVKLPKTATDAELKMIAGSIL
                     LALSLILFVFNRRQMSPS"
     gene            10068..10670
                     /locus_tag="VN968_27330"
     CDS             10068..10670
                     /locus_tag="VN968_27330"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class GN sortase"
                     /protein_id="HXL32979.1"
                     /translation="MPRFAVPLLLALAGLVLFGQGAYIHAKALLAQVLLEHAFAETIA
                     SGRVTKPWSWADTWPVARIEVRRLRASAIMLAGSSGQALAFGPGHVELTPNAGERGVA
                     VYSAHRDTHFRFLKNVAIGDEIDIMRNDGRSFRYRADGAAEVRFDASGIDPLTDGYEL
                     VLSTCWPFDAVTPGPTRYVLHATMIGLSGTSDEQVPNGSN"
     gene            10848..11567
                     /locus_tag="VN968_27335"
     CDS             10848..11567
                     /locus_tag="VN968_27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HXL32980.1"
                     /translation="MSSQVHYLPLTPGLFSILVLLFVVLLILIQLRILRYAYMRLGIG
                     PGMALLLLFGSLIGSYFNIPITVLAGPAVKSGQIVEFFGMRYIAPVVVAWPGTVLAIN
                     VGGAVIPTVMSAYLMFRYQFWLKATLVTAAIALVIHSMATPVPGIGIAVPVFAPVMTT
                     AILAFLLSREYAPPLAYIGGSMGTLIGADLMNLDKITGLGAPVASIGGAGTFDGIFLT
                     GILAVLLAGVASPSRPPRPAW"
     gene            11722..>12460
                     /locus_tag="VN968_27340"
     CDS             11722..>12460
                     /locus_tag="VN968_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXL32981.1"
                     /translation="MEAQVSIASDSTVKWSPSSDASDIVKRIHAMLHPRNIVLVGATD
                     KPGNYAERIWNNLIKYQYEGGLYPVNAKRETIWGVPCYKDFASLPEEPDHVLVLVPAR
                     FTVQVIRDAAAAGARSATIVTSGFSELQDDESQRLAAELQAAIRETGLAVTGPNCLGN
                     LSAGERLFTNIDDRVVTMEAGPVAIAGQSGAIVMAIRQALEDRGVGVGYMVTTGNEAG
                     LETPDLMAYFAADPSIRVIVVYLEGVRN"
BASE COUNT         2391 a         3946 c         3797 g         2326 t
ORIGIN      
        1 cgatcgccac cagcagcgcg gcccacgcca gcacggccca tggccacggg gacccgatgc
       61 cgcccaggga ggcggctccg cccgttgtac gcgctggagc ttcgcggtcg gaccgcacga
      121 tcgccgtcca cacgggatga agttcgaatg ccgccccagc ttcatgctag accaaaacgc
      181 tccacaagac cacggcgtgc tcccggctcc ctcgcctccc cctcaatacg cgcgtctcag
      241 acgccgccga atcgacagcg ctccgtcttc aacggcgtgt ggcgacggag atcgtggtgg
      301 ccgtcgtccg atatcgtcga tccccgacca gcccagaaaa tcgaaagaca ccctgacgag
      361 cgacattcgg atgccgccaa cggcgttcag cgatcgctcc attgacccaa caggggtgga
      421 ggttcccgca tgtgtcctat ctgccgcact gtagatctat ccctcgaacg tgttctgtct
      481 gccgcactgt ggacgtattc cgacgttcat gctcgtggcg tctctgagag gaacggtcag
      541 cgggctactg tcttgtttct tctaacctgg aggagaagta ccgtgatgaa aattgtgatg
      601 atagcgggtg caacgctagc cctggtgact gccgccggcg ccgccgacct tccacaccca
      661 cagcccgtct atcagacggc tcaactcggc aagatgccga ttggcaaggc cccgatcggg
      721 aagagcccaa tcggcaaggc gcccgtcgtg acgcggagct actaatccgc ggtatcgact
      781 gaaggtctcg ttgaatctga cctttatcgt cgacagctga agggcgaagc gtggcaaaca
      841 ggccgaagag attgggacga tgcttgcttg cgggattcac gctcgctgtc ttgctccagt
      901 gcaagatcca atcggctgct gcacacgaga ttgacactcg cgccggttcg gaacgtttca
      961 tctcccagaa acgggaggtc cagaagcgca ccgccgacgc gaatccgctt gtttccagtg
     1021 aggcgccgat tgaatcgaaa ccggcgccgc ggtcgactcg aaagtcgggg tcggggtcag
     1081 caaaaggccc gtattacgtt gatttcagag ccagaacggc ggcgagctgg ggacacgctt
     1141 tcgtctggtt tgggaagacc agcgagcgag aagttgaggt cgcgggcctt acccccgcgg
     1201 gagacaacct ggcttatgtg ctgggtcatg tgacgtgggt gccgtccgaa accggggcaa
     1261 gctatggcga tcttgatccg cagtacttga ccgccagtta ccgggtttac ctgaatgagc
     1321 cggacgcaaa aagggttttc gcatacatca agaaattaca gacgagttca ccggtctgga
     1381 acgccgaaac atccaattgc acttcgttta tcggcagcat cgcaaatttc atgggattga
     1441 aggttcctct ccgctggcag cgcccggagg actacgtcaa cagtctcaag acgatgaatg
     1501 gcgggagcca gatcgtccac ctgtcgtcgg aacaatagct cgcgttcgtc cttggccccc
     1561 taaaggatgc gccttcgcgt caccggtcga agatcagtat ctgttacgtg agactccgaa
     1621 gtccggtttg acgaacgcat cgccctaatc gcggccacat cgcaggtcgg gattattgtg
     1681 ggaccgccgt tggcggagaa ccttctggcg ccagccgagc cacgcgatga ggccattccc
     1741 agccacttaa cgcaagacag catcccggga cggcgtccca actggcgcga tatggcatag
     1801 catcaaacag gaccaggcgg cgctagaccg ctgaaccttc ggaggagatt ttgccggttc
     1861 gccactatga ctggatcgcg cattttgccc gccggacgcc ggacaagctg gcggtagttg
     1921 accttgccag cgagcgccgg cttacctacg cgcagttcga cgcgcggatt tcgcatctcg
     1981 ccacgcatct gcgcgacggg gtaaaaatcg cgcgcggcga ccgggtcgcg gtgctggcac
     2041 acaacaccac cgatacgctg gaggtgcagt ttgcctgtgg gcggataggc gcggtgttcc
     2101 tgccgctgaa cacccgcctc accgtgcccg agctttcata cattgtcggc gatgccgcgc
     2161 cgcagcttat gatccatgac gccgcgttgg cggatctcgc gcttgccgtt gcaaaactgt
     2221 gcgaggtccc ctcggcgctg ctgctcggtg caggcggttc ctttgaggcg gcgatcgcgg
     2281 cggcgaaacc gctcgaccag gcggaaatcg tcaccctcga tgacatgtcg acgatcatgt
     2341 acacctcggg caccacgggc cagccgaagg gcgccatcat cacccacggc atgacgttct
     2401 ggaattgcgt caatctcggc ggtccggcct atatctcgcc ctcgtcggtg ctgctgaccg
     2461 tgctgccgct gttccacacc ggcgggctga attgctacac caacccggtg ctgcatgccg
     2521 gcggcaccgt gctgatcatg cgcgcgttcg atccgggcct ggcgctgcaa ttgatcagtg
     2581 acccatctca ccgcatcaac acgtttttcg gcgtgccctc gatctatcag ttcatggcgc
     2641 agcatccggc gtttgccggt tccgacttca gccgcctcgt catctgcggc gtcggcggcg
     2701 cgccgatgcc ggtgccgctt ctgaaagtgt gggaggagcg cggcgtcgcc ttgcaacagg
     2761 gttacggcat gaccgagacc agcccggcgg tactgacgct cgaccgcgag gatgccgtgc
     2821 gcaaggcggg ctcctccggc aagccggtgc tgcacaccga agtgcggatc gtccggcccg
     2881 acggcgtaga cgccgacatc ggcgagctcg gcgaattatg ggtcaaggga ccgaacatca
     2941 cgcccggcta ctggaacagg ccggacgcca atcaatcttc cttcaccgat ggctggctgc
     3001 acaccggcga cgcggcgcgg atcgacgaag aaggttttta ctacatcgtc gaccgctgga
     3061 aggacatgta catttccggc ggcgaaaacg tctatccggc cgaggtagag aacgtgctgc
     3121 accaactcgc cgcgatcgcg gaagccgctg tcatcggcat tgccgatgcg caatggggcg
     3181 aggtcggcat ggcgatcgtc gccgtcaaac cagggcactc cctgaccgaa gccgagattc
     3241 aggcgcattg cgaggccaac ctggcgcgct tcaagcggcc ttgcgtgatc cggttcgtcg
     3301 aggccttgcc gcgcaacgcc accggcaaga tccacaagcc gacgctgcgc cagaatttcg
     3361 gcatccccac agctaccgac agcgccaatg ccgttgcctc ctgaccgctc gctcgcgacc
     3421 agcccgtccg acgtcaatcg cttgatcaag aaaaggtact cgccatgaac aacaagaaac
     3481 ggtccctgct cgcctcccac ctgattgcgg tcgccgccgc cacggcgctt tcggtgctgg
     3541 ccacccaagg cgcgctggcg cagaagaaat acgacaccgg cgccaccgac accgaaatca
     3601 agatcggcaa tgtcgaggcc tatagcgggc cggcttcagc ctacggcatc atcggcaaga
     3661 ccgaggacgc ctatttcagg atgatcaacg aacagggcgg catcaacggc cgcaagatca
     3721 attttatcag ctacgacgac ggctacagcc caccaaagac ggtggagcag gtccgcaagc
     3781 tgatcgagag cgacgaagta tttttggtct tcaacgcgct gggcacgccg acccagaatg
     3841 cggtccagaa atatcacaac gccaagaaag tcccgcaact gttcctcgcc accggcgcca
     3901 gcaagtggaa cgacccgaag gattatccct ggaccatggg ctttcagccg agctaccgcg
     3961 tcgaggcccg gattttcgcg aaatacatcg tgaagacaaa gcccgacgcc aaggtcgcgg
     4021 tgttctatgc caatgacgac ttcggcaagg actatgtggc cgggctgaag gagatttttg
     4081 gcgacaaatc gcccagcatc cttgtcgccg aggaaagcta tgagaccacc gagccctcga
     4141 tcgattccca tatcgtcaag ctgaaaggca ccggcgccga cgtgttcgtc aatatctcga
     4201 cgccgaaatt cgcagcccaa gcgatcaaga agatggcgga actggaatgg aagccgatgc
     4261 acgtcatgac cgacgtctcg atctcgatcg gcgcggtcat gaagcccgca ggcctcgagg
     4321 cttccgaagg catcctgtcg gcgggctatc tcaaggacgc ctccgatccg cagtggaaag
     4381 acgatgaagg catgaaaaag ttcatgacgt tcatcgacaa gtactcaccc ggcgcgaaca
     4441 tctccgacgc caatctggtg tacgcctatg ccgcggcgca gaccatggtg caggtgctgc
     4501 agcagtgcgg cgacaacctg acccgggaaa acgtgatgaa gcaggccgcc agcctgaagg
     4561 actttgcgcc ggacacgctg ctcccgggta tcaagatcgg taccagcgcg acagactttg
     4621 ccccgatcga acagctcaag atgatgcagt tcaagggcgg caaatgggac ctgttcggcg
     4681 acatcatcag cgccgagacg ggtggctagt ttcaaacccg tattctgaaa gtatgaccta
     4741 aggatacggt gagcatttcc gatgctctcc gacatttcga aaaggatccg tctttttcgt
     4801 catggccggg cttcgtcccg gccatggcga gattctattg gctgcatttc gcagtcaggc
     4861 tcttgggatg acaagttttg agtccgtcgc tttggtccaa ccctatgccg ccctagcccg
     4921 cctcaaacga cgccgtaatc gcggcctgag cctgttgctg aatctgccgc agatgttgct
     4981 ggcttatgaa actttcggcg tagatcttgt agacatcctc ggtgccggag ggtcttgcgg
     5041 caaaccatcc gttttccgcc actaccttga tgccgccgaa cgattgattg ttgccgggag
     5101 cagcattcag cgtcgcgcgc agcggctcgc cggcaagctc cttgatggcg agttgctccg
     5161 gcgacagcgc cttcagcaga ttcttttgcg cgaccgtcgc aggcgcgtcg atacgctcat
     5221 agaacggcac gcccagttcg ccggtgagac ggtcgaatag ctgactggga tcctgtttgg
     5281 tccgcgccgt gatctcggcg gccaatagac caagaatgat gccgtccttg tcggtggtcc
     5341 acaccgaccc atcgcgccgg acgaatgagg cgccggcgct ttcctcgccg gcaaaaccga
     5401 acgacccgcc gatcaggcca tcgacgaacc atttgaagcc caccggggtc tcgaccagcc
     5461 tgcggccgag ttttgcggcc acgcgatcga tgatcgcgct gctgacgatc gtcttgccca
     5521 cggcgctgtc cttgctccag cccgggcggt tctcgaacag ataggcaatc gcggcggcga
     5581 gataatggtt cggattcatc agcccgttgg agcgggtgac gatgccatgc cggtcggcgt
     5641 cggtatcgtt ggcgaaagcg acatcgaact tgtcgcgcat gccgatcagc cgcgccatgg
     5701 catagggcga cgagcaatcc atccggatct tgccgtccca atcggccgtc atgaaccgaa
     5761 acgtcgggtc caccgtgtcg ctgacgatgg tcgcggcaat gccatatcgt tcgatgatgg
     5821 gctgccagaa atgcacggcc gcgccgccca ggggatcgat cccgattttc acccccgagg
     5881 cgcggatcgc ctccatatcg atgacattgg caaggtcggc cacgtagggg ccgatgtaat
     5941 cgtagcggtg aacgcacctg gccttgtggg cgcggtgata cgaaatccgg tgcaagcctc
     6001 tcaagccgtc ttccaggaat gcgtttgcgg cgcgctcgat tccggtcgtg acatcagcat
     6061 cagccggtcc gccatttggc ggattatatt tgaagccgcc gtcctcggga gggttatgag
     6121 acggggtgat cacgacgcca tcggcaagac cggttgtccg atttttgttg taggtcagga
     6181 tcgcatgcga gatcgcaggc gtcggcgtgt agccgtcgcg ctcgtcgatc atcacggtga
     6241 catcgttggc tgcgaatacc tcgagcgcgc ttgcgaaggc gcactccgcc aaggcatggg
     6301 tgtcgatgcc gatgaatagc gggccgtcga tgccgctttg gcgccgatgg tcgcagagcg
     6361 cctggctgat cgccagaata tgcgcctcgt tgaaggcgtt gttgaaggcc gagccgcgat
     6421 gcccggaagt cccgaacgcc acgcgctgcg acgggatttt gggatccggc ttgtttgaga
     6481 aataggccgt caccagccgc ggcacattga ccagcatcga tgctggtgcg ggtttgccgg
     6541 cgagggggtt tacttcgacg gccattgatc tctccttctt caattgaatc agactctagc
     6601 ccggcaagaa cacggcgaat ggctcctcgg cgatgcgccg caggtgctcg cgatagctgg
     6661 cgtagctggc gtcaccaggc gaaatccgtc cgaacgccgg actcaagaac gcctcgatcc
     6721 gcaaaagcgc aatgggcgcc gcaatcggcg tcagcagcga gccgcgcccc gccagcagcg
     6781 tcgtgatgat gccgtacatt tcgccggcct ttcttggaag cgacgccatg atgaggcgat
     6841 gctggccatg ccggttggtg atgagataga tgtggcccga ctggttcggc accgcgactt
     6901 cgccgaactg ggtgtaggcc gcgtcctgcc tttcgccttc acggaacacc agcgatgaaa
     6961 ccgcgggatc ccaggcgatc tcggtgcgat aggcgtagat cgcgtccttt tcgccgaacg
     7021 acggccgcac cgtgacatag acgccttcga gccactcaac cgagcggcgc gaataagccc
     7081 cgaggccgtc gggggagacg tcgccattga ccgggaccgc ggccggcgcg ctgtcctgtg
     7141 ccttgcgcaa ggagacgccg agcgcctgtt cgagccgcac ggtggtggcg agcgtgaagg
     7201 gacggcggcc gcccagcacc ttttccagcg tcgagaggct gagcttggcc tgctcggcaa
     7261 gcgactgccg ggagatgcga cgccgcgcga tttcctcgcg aatcgtctcc gccacctgcc
     7321 ggctttgctc gaccgaaagc tgcttgtccg gcgtcagcat cgtcacccct ttcgaactcc
     7381 accatcctag cagaagcaca attcatcaca aagccgcaca aaaccgcctt ggcggcggcg
     7441 cgccgaagcg gctcgcggct gaacattacc gatcattctg ctcgcgccaa aacacccggt
     7501 tccgcacaaa cggcaggcga tgaaaccccc gcgggagtta cagaaatgac cacatgcgat
     7561 gaaattgaga ccgccgagag ctcgggccta tggcgtatcg taagactgct gttgttttgt
     7621 gtgatgcagg gcgccgccgt catcctggtc ggatttgcgg cattattctt gagctttgag
     7681 ccggtgtggt cggcggagaa tccgtccgca accctggcga gcctcagcga cgcgcgctcc
     7741 ggctccctgc tcttgaaaac cgatgacggc tatgccgatg cgaccaggct cggcatcgac
     7801 gtcgatctca ccgtatcggg tcccaccgtc cgtgcccgcg tcacgcagat cttccgcaac
     7861 ccgacgaaaa actgggtgga ggcgacctac gtctatccgc tgccggcggg cggcgcggtc
     7921 gacacgctca aaatggtgat cggcgaccgc gtcgtggtcg gcgatatcaa ggagcggcag
     7981 caggcccgca tcatctacga gcaggcgcag cgcaacggac aaaaggccgc attgaccgaa
     8041 caggagcggc cgaacatttt taccaactcg gtcgccaata tcggtcccgg cgaaaccgtg
     8101 ctggttcaga tcgaatacca ggaacccgtg cagcagatgg gcaatgaatt ctcgctgcgg
     8161 gtgccgatgg tggtcggccc gcgctacaat cctgcaccgg tcgtgcagag cgtcgacttt
     8221 cggcccaacg gcggcggctg gggcgtgaca tccgccgatc cggtgccgga ccgcaaccgc
     8281 atctcgccgc ccgtgctcga tcccgccgaa catgggccgg tgaatccgac cgcgatcacg
     8341 gttcggctgc aggccggctt cccgctcggc gaagtcaaga gccatcacca tcaggtcaag
     8401 atcgacagcc ccgacgacag cacgcgcgtc atcaggctgg ccgagggcgt ggtgccggcg
     8461 gatcgcgatt tcgagctgag ctggaagccc gcggcggaga aggcgccgtc ggtcgggctg
     8521 ttccgcgagc acgtcggcga tgccgattat ctcctggcat tcgtcacgcc gccgtcggtc
     8581 gaagcggccg agcaaaagcc gctgccgcgc gaggtgattt tcgtgatcga caattccggt
     8641 tcgatgggcg gcacctcgat catccaggcc aaggcgagcc tcacctacgc gctcggccgc
     8701 ctgcagccga acgaccgctt caatgtgatc cgcttcgatc acaccatgga cgtcctgttc
     8761 ccgcatccgg tgcccgccga tgcggaacat gtcgccagcg ccaaatcgtt cgtcggcgca
     8821 ttgcaggccg aaggcggcac cgagatggtg ccggcgatgc aggcggcgct gaccgacaat
     8881 gccgccggcg acgccaacac cgttcgccag gtggtgttcc tgaccgatgg cgcgatcggc
     8941 aacgagcagc aattgttcga caccattacc gcgatgcgcg gtcgctcccg cgtgttcatg
     9001 gtcggcatcg gctcggcgcc gaacacgttc ctgatgaccc gcgccgccga actcggccgc
     9061 ggagccttta cccacatcgg ttcggtcgaa caggtcgagg agcggatgcg cggcctgttc
     9121 gccaagctcg aaaatcccgc cgtgaccggc cttgccgcga agttctccga ggccacggcc
     9181 gacctcgcac cggcggcgat ccccgacctc taccgcgacg agccgctggt tctcgcggcg
     9241 aaactcgaca cgctcaaggg ctcggtcgag atcaagggcc ggatcggcga ccggccctgg
     9301 gtcgtgacgc ttgccctggc gaacgccgcc gagggcaagg gcctctccaa gctgtgggcg
     9361 cggcgcaaga tcgacgacgc cgagatcgcc cgcaccctgc gaaccgcgac gcccgaggac
     9421 gccgacaaga ccatcctggc gctggcgctg cagcatcagc tcgtgagccg gttgaccagt
     9481 ctggtcgcgg tcgacgagac cccgagtcgt cccgaagggg aagcgctcaa gctcagtgaa
     9541 ttgccactca acctgccggc aggctgggat tttgcgaagg tgtttggcga gcgcccaaga
     9601 cttccgtcac cgccggccga gcgtcgcgcc gacgccggcg acgcccgggt tcatctcgcg
     9661 gccgcgaagc gcatgcccct cgccgtcacg ccggcgcccg gcaccgtgaa gttgccgaag
     9721 acggcaaccg atgccgaact caagatgatc gccgggtcga tcctgctggc gctcagcctg
     9781 atcctgttcg tgttcaaccg acgtcagatg tccccgagtt gacgccggat gaagggagga
     9841 gttgtccccc ctcctcctca aagcgcgcgc ggcctcccac ccacctccag ggccgcgcgc
     9901 gctgcgattt atcgtcattg cgagccaacg ggtcgcgcga atccgcgccc gatgacaggc
     9961 tccgcaccag gcctcagatg tgcaattgca catcggggaa tctcgagatt ccgggttcga
    10021 tgctgacgca tcgccccgga atgacagaaa ccatattgag attctcaatg ccccgcttcg
    10081 ctgtaccgct gcttctcgcg ctcgccggcc tcgtgctgtt cggtcaaggc gcttacattc
    10141 atgccaaggc gctattggcc caggttctgc tggagcacgc ctttgccgag accatcgcca
    10201 gcgggcgcgt gacaaaacca tggtcctggg cggatacttg gccggtcgca cgcatcgagg
    10261 tgaggcggct tcgtgccagc gctatcatgc tggccggaag cagcggtcag gcgcttgcct
    10321 tcgggccggg tcacgtcgag ctcacgccca atgcgggcga gcgcggcgtg gcggtctatt
    10381 ccgcgcaccg cgatacgcat ttccgctttc ttaagaatgt cgcaattggc gacgagatcg
    10441 atatcatgcg aaacgacggc cgcagcttcc gctatcgcgc ggacggcgct gcggaggtgc
    10501 gtttcgatgc ctcgggcatc gatcccctaa ccgacggata cgaattggtg ctgtcgacct
    10561 gctggccatt cgatgcggtg acgccagggc cgacgcgcta cgttttgcac gccaccatga
    10621 tcgggctaag cggcacttcc gatgaacagg tgccgaatgg ttcaaattga gttcggtgct
    10681 ggtcaagaag cgggccgagg ctttttgccg cacgccggca ggggacgtta tgccggttca
    10741 tggccaatcc catccgtgct tgaatgttgt tctgctacac tcttgccgtg cggcggaaaa
    10801 tcaataattg aaacatgaca aacaaccggg attgaaccag cgaaatcatg agttcccaag
    10861 ttcactattt gccactgaca ccgggattgt tttcgatcct ggtcttgctg ttcgtggtac
    10921 tgctcatcct gattcagctt cgcattctca gatatgccta tatgcggctc gggatcggtc
    10981 ccggtatggc ccttcttctg ctgttcggct cgctgatcgg cagctatttc aatattccga
    11041 tcacggtgct ggccggacca gccgtcaaat cgggacagat cgttgaattc ttcggcatgc
    11101 gctatattgc gccggtcgtg gtggcgtggc ccggcacggt tctcgccatc aacgtcggcg
    11161 gcgccgttat tccaacggta atgtccgcgt atctgatgtt tcggtatcaa ttctggctca
    11221 aggccacgct tgtcaccgcg gccatcgcct tggtgatcca ttcgatggcg acgccggtgc
    11281 cgggaattgg tatcgccgtg ccggtgtttg cgccggtcat gaccacggcg atccttgcat
    11341 ttctgctctc ccgcgaatac gcgccgccgc tggcctatat cggcggctcg atgggaacgc
    11401 tgattggcgc cgatctcatg aatctggaca agatcaccgg cctcggcgct ccggtcgcct
    11461 cgatcggcgg cgccgggaca ttcgacggca tcttcctgac cggcattctt gccgttctgc
    11521 tcgccggtgt cgcctcgccg tcgcgcccac cacgaccggc gtggtaagtc cctctacggg
    11581 tggctgcggg catgggcccg ttccgcgagt tgccaccctc cccgactatc aataataaga
    11641 aatcaaaagg tcgcggggaa cgtcgatctc cagtcgatcg ccatttttcc gcataagaaa
    11701 ccaagggtaa gggttacgcc tatggaagct caggtcagca tcgcgtccga ttcgaccgtg
    11761 aaatggtcgc cttcctctga cgcaagcgac atcgtcaaac gcattcatgc aatgctgcat
    11821 ccgcgcaaca ttgtgctggt cggagcgacg gacaagccgg gcaactacgc cgaacggatc
    11881 tggaacaatc tgatcaagta tcagtacgaa ggcggtctct acccggtcaa tgccaagcgc
    11941 gaaaccatct ggggcgttcc ctgctacaag gatttcgcca gcctgccgga agagcccgat
    12001 cacgtgctgg tgctggtgcc ggcgcgtttc accgtgcagg tcattcgcga tgccgcggcg
    12061 gccggcgcgc gttcggcgac catcgtgaca tcgggcttca gcgagctgca ggacgatgaa
    12121 agccagaggc tcgctgccga actgcaggcg gccatccgcg agaccgggct tgcggtcacc
    12181 ggcccgaatt gtcttggcaa tctgagcgcc ggcgaaaggc tgttcaccaa catcgacgac
    12241 cgtgtcgtca ccatggaggc aggaccggtc gcgatcgccg gtcaatccgg cgcgatcgtc
    12301 atggcgatcc gccaggccct ggaggatcgc ggcgtcggcg tcggctacat ggtcaccacc
    12361 ggaaacgagg ccggcctcga aaccccggac ctgatggcct atttcgcggc cgacccctcg
    12421 atccgcgtga tcgtggttta tctcgaaggc gtgcgcaata
//
LOCUS       DATNOI010000682         2010 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1685855,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000682 DATNOI010000000
VERSION     DATNOI010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685855"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VN968_27345"
     CDS             complement(<1..1058)
                     /locus_tag="VN968_27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL32982.1"
                     /translation="MAPKPPSSDDQVPDSADKPVAPELDRKLAAGAERVIGDKTAGSP
                     PPDDEDEDEDELDIEDEDDDEDLVVFTAKEAAGALATVYAFVKPYLKNYKKILAFVGF
                     GVLVETLFNVIMPLSLKFLIDDALGEEDFEALVKILSALAAAGIVTSVVAVWYERWDA
                     ELAATLISDVRLRLFEHVQNLPSAYFARTKRGEILSRFSIDLSAFEGSIKSFANSAAL
                     PFFELIAGIVLMLFLNWQLAAVALLVFPITLIGPRILTPKAVQANYEQKINESALLGM
                     VQENVAAQAVIKAFSLQRRTFGWFTMRNDDARRKIAAAAFLSTMVERTVTISVLLLHL
                     VVLALGAYLATKGQITIGT"
     gene            complement(1216..>2010)
                     /locus_tag="VN968_27350"
     CDS             complement(1216..>2010)
                     /locus_tag="VN968_27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXL32983.1"
                     /translation="GDYLFDSTLNGLLDSSDAATDIILTEMMRLRRARGEDTRPASDK
                     IDRKYFENYRGLGPYQEVWNRFTDPAYLTDLIKIVWNKARGYRLLVAGSASGELVGAL
                     RDRGIDAWGIENNRAIHARTPKALKKFNKLGSIVDMPFKDGEFDFVFETSLCHVAEKQ
                     VPRAVRELNRVVKTGLVFGSVTSDMAPALIDQYDLLRGVKKLGTWWEWSELFFSNGFD
                     LSMHRRDCTDALWAATLAANKGPGQWYADSDSLRYSFFDKVKADDE"
BASE COUNT          400 a          636 c          591 g          383 t
ORIGIN      
        1 gtcccgatgg tgatctggcc cttggtggcg agataggcgc cgagcgccag caccacgagg
       61 tgcagcaaca gcacggagat cgtgaccgtg cgctcgacca tggtcgacag gaatgccgcc
      121 gctgcaattt ttcggcgcgc gtcgtcgttg cgcatcgtga accagccgaa cgtgcggcgc
      181 tgcaagctga aggccttgat gaccgcctgc gccgcgacgt tttcctgcac catgccgagc
      241 agtgccgatt cattgatctt ctgctcgtaa ttcgcctgca ccgccttcgg cgtcagaatc
      301 cgcggaccga tcagcgtgat cgggaacacc agcagcgcca ccgcggccaa ttgccagttc
      361 aggaacagca tcaggacgat cccggcgatc aactcgaaga aaggcaacgc cgcgctgttg
      421 gcaaaactct tgatcgaccc ttcgaaggcc gacagatcga tcgagaagcg cgagagaatc
      481 tcgccgcgct tggtccgcgc aaaataagcc gatggcaggt tctggacgtg ctcgaacagg
      541 cgcaaccgga cgtctgaaat cagggttgcg gcgagctcgg cgtcccatcg ttcgtaccag
      601 accgcgacga ccgaggtgac gatacccgca gccgccagtg ccgagaggat cttgaccagc
      661 gcctcgaaat cttcctcgcc aagcgcatcg tcgatcagga atttcaggct cagcggcatg
      721 atgacgttga acagcgtctc gaccaggacg ccgaagccga cgaacgccag tattttcttg
      781 tagttcttca ggtatggctt gacgaacgca tagaccgtcg cgagcgcgcc ggcggcttcc
      841 tttgcggtaa agacgacgag atcctcatcg tcatcttcgt cctcgatatc gagttcgtct
      901 tcatcctcat cttcgtcatc gggcggaggc gagcctgcgg tcttgtcgcc gatgacgcgc
      961 tcggcgcccg ctgcgagctt ccggtccagt tccggcgcaa ccggtttatc cgcggaatcc
     1021 ggaacctgat catccgacga tggaggcttg ggcgccatga gaccaaccgt tgctgcacgg
     1081 ccagcatgac cgcaaaatcg agggagatgg ccgagagtgc caccgcgtcg atcctatgcg
     1141 attggcacgc attcggcaaa gcaacagcga gaaactttcc acgagtcgtc cccgcgaacg
     1201 ccggggacgg cgcgtttact cgtcgtcagc tttcaccttg tcaaagaacg agtagcgcag
     1261 gctgtcggaa tccgcatacc actgacctgg ccccttgttg gcggccagcg ttgcggccca
     1321 gagcgcgtcg gtgcagtcgc ggcgatgcat cgacaggtcg aacccgttgc tgaaaaacag
     1381 ctccgaccat tcccaccagg taccgagctt cttgaccccg cgcagcaggt cgtactggtc
     1441 gatcaatgcc ggcgccatgt ccgatgtcac cgatccgaac accagcccgg tcttgacgac
     1501 acggttcaac tcgcgcaccg cccgcggcac ctgcttttcc gcgacatggc acagactggt
     1561 ctcgaacacg aaatcgaatt cgccgtcctt gaacggcatg tcgacgatcg atccgagctt
     1621 gttgaacttc ttcagcgctt tcggcgtgcg ggcatggatc gcgcggttgt tctcgatgcc
     1681 ccatgcatcg atgccacgat cgcgcagcgc acccaccagt tctccgctgg cggatccggc
     1741 caccaggagc ctgtaacccc tcgccttgtt ccacacgatc ttgatgaggt cggtgagata
     1801 ggcaggatcg gtaaaccggt tccagacctc ctgataggga ccaagcccgc gatagttctc
     1861 gaaatacttc cggtcgatct tgtcggatgc aggcctggta tcctcgccgc gcgcgcggcg
     1921 cagccgcatc atctcggtga gaatgatgtc ggtggcggca tcggaggagt cgagcagtcc
     1981 gttcagcgtc gagtcgaaca gatagtcgcc
//
LOCUS       DATNOI010000683         6821 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_95273,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000683 DATNOI010000000
VERSION     DATNOI010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95273"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VN968_27355"
     CDS             <1..223
                     /locus_tag="VN968_27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL32984.1"
                     /translation="LVLKGVLDIQLRDRTVTLGPGQMYIVPKGVEHCPVAKEEVHLLL
                     IEPTGTPNTGDVKTAAPRKTTARAHEPAP"
     gene            complement(313..1605)
                     /locus_tag="VN968_27360"
     CDS             complement(313..1605)
                     /locus_tag="VN968_27360"
                     /inference="COORDINATES: protein motif:HMM:NF020416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HXL32985.1"
                     /translation="MIVVDQPDALMDVPVAIELREFTPRQAVTVTATQIFPSHSRWQA
                     HATFMTDESGAVGIARQAPISGSYEGVAAMGLIWSAERVSPPSPPFPDDWVLQPSFIH
                     FEAVGPNHQRAELTITRRSAGPGVTRRVIRIDGLAGILFLPPGEGPHPSVLILHGGGG
                     GMDEYSGAMLASRGYAAFNLAYFAEPGLPRGLVNIPLEYFEHAIRWMRAQPWLRDGFL
                     AAWGPSRGGELALLLGATFPDINAVSAWVPSGVMFWPIGLAEQGDTQPPASWTWRGKP
                     LPYLQENNGSVEPLPPQRPGQPTAYTPVYLRHLRDERAVERATIPVQKIRGPVLLVSG
                     TDDQMWPSSVLADIATRRLESNGFKFPFRHLKYEGAGHLILLPWGPRTTHNIGFKAEG
                     FSGLVYAQGGTPRTDAEAGADAWHQMLHFLEAGASDRR"
     gene            1806..3251
                     /locus_tag="VN968_27365"
     CDS             1806..3251
                     /locus_tag="VN968_27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HXL32986.1"
                     /translation="MSRSAKAEAVLALHRFGMGPRPGSIAAIEADPRGALIAELERPS
                     ASQLAASALPSSSKAFCTVNEANAKRRAKTIIAAKAQQEAKRQQMSDAPSMMEGSEQD
                     ANAMAAKIAAEAVPDPGRQIYLDEARTRTEAALGTEIGFVERLVWFWSNHFCISADKI
                     QSMSGAYEREAIRPHVVGRFADMLLAVEGHPAMLFYLDNTDSIGPNSIAGINRTRGLN
                     ENIAREILELHTLGVRSGYSQDDVLSFAKVLTGWTLLQPANNPEHGGEFSFNPRLHEP
                     GAQKVIGKVYEDETVEQGRGVLRDLAAHPATATHLATKLARYFIADTPPPPLVERMAK
                     VFADTAGDLKEVAKAMVSSDESWTQPPTKLKRPSEWVVGMVRAAGILQADPARFTAGQ
                     ALLGEPLWRPTSPKGYPDDEASWIDGMGRRLDVANNFAERVSSRTDPQEVIETVLGSS
                     VSPEVMQAVGRAETRQQALVLLFMSAEFQRR"
     gene            3254..4504
                     /locus_tag="VN968_27370"
     CDS             3254..4504
                     /locus_tag="VN968_27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HXL32987.1"
                     /translation="MTFCHHLPSRREALIGSGALFAWSQMPRLARAAGRDPRMLVIVL
                     RGALDGLGAVAPVGDPDWIGLRGDRALVLDGKTPALPLDAFFALNPAMPNLHRMYKAQ
                     QASIVHATATPYRERSHFDGQDVLESGIARPGAVDTGWLNRALVALASDGQVDLRGSR
                     ALGVGSVTPLVVRGKAPVMSWVPQQLLPASDDTQARLLDLYQHTDPRLAVALEARIRL
                     AALGPARGPNDSMSDGASLPPPGIARVRAYFADAAGTAARFLAKADGPRVGAVGFVGW
                     DTHISEGAASGQLANLLGALDGALAAIETNMGDAWRETVVAIITEFGRTARINGTDGT
                     DHGTGTVAFLAGGALRGGRMVADWPGLKPAQLYEGRDLKPTTDLRAVLKGLLRDHLRV
                     EESVLASTVFPDSAEVVPTAGLVG"
     gene            4656..4844
                     /locus_tag="VN968_27375"
     CDS             4656..4844
                     /locus_tag="VN968_27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32988.1"
                     /translation="MSIFTGRELEFLASLRTSWRRLYWNELARLLKEDDNACDRHEAH
                     SIAPQSEGSKGFVPNQAT"
     gene            complement(4848..6236)
                     /gene="gor"
                     /locus_tag="VN968_27380"
     CDS             complement(4848..6236)
                     /gene="gor"
                     /locus_tag="VN968_27380"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HXL32989.1"
                     /translation="MAEFDVDLFVIGGGSGGVRAARIAAGYGARVMVAEEYRMGGTCV
                     IRGCVPKKLFVIGSHIHHEIEDAAGFGWSIPPVTFDWPTLVANKDKEIARLEGIYTTN
                     VEKSGARIVKTRAVLEDAHTLRLTNGEAVRAKYVLIATGSAPNHGPAIPGIEHVISSN
                     EVFHLRELPKRIVIQGGGYIALEFACIFAGFGSDVTLVYRGDNILRGFDEDVRAHVRA
                     EMEKEGITILTGCTVNKVDKPGKEFISHLSNGSSIASDQVMFAIGRHPNVANLGLENA
                     GVAINPGNGGIAVDGFSQTSVPNIYAIGDVTHRVNLTPVAIREGHAFADTVFGKHPVQ
                     VDHSNIPTAVFSQPEVGTVGLTEAQARAQFSHVDIYKADFRPIKSTMSGRDTRILMKL
                     VVDGSSDRVVGCHIVGDTAAEIVQAVGIAVKMKATKADFDATFALHPTAAEELVTMRT
                     PTARYVREAAAE"
     gene            complement(6571..>6821)
                     /locus_tag="VN968_27385"
     CDS             complement(6571..>6821)
                     /locus_tag="VN968_27385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HXL32990.1"
                     /translation="SLAGREKEIGEGMAKIYANEFSEQELKDLVTFYKSTLGQKLLAT
                     EPRAIQSSMSYMNQWAQNFAEVVNSQFRAEMRKRGKEI"
BASE COUNT         1281 a         2141 c         2124 g         1275 t
ORIGIN      
        1 tctcgtcctc aaaggcgtgc tcgacatcca acttcgagat cgaacggtca cccttgggcc
       61 ggggcaaatg tacattgtac cgaaaggcgt tgagcattgt cccgtcgcga aggaagaggt
      121 gcatttgctg ctgattgagc cgaccgggac acccaacacc ggtgatgtca agacagcggc
      181 gccgcgcaag acgactgcgc gcgctcatga gcccgcgccc tagctagggc gcttactcac
      241 gaatcgaggt ctttcggtga gaccttgttg atgtccgcat ccatgcatgc ggttgggtaa
      301 accagatcgc gtttatctgc gatcgctggc acccgcctcc aggaagtgca gcatctgatg
      361 ccacgcatcg gcaccagcct cggcgtcggt cctcggcgtg ccaccctgcg cgtacacaag
      421 gccgctaaag ccttcggcct tgaaaccgat gttgtgcgtc gttctcggtc cccagggcaa
      481 caaaatgaga tgacccgcac cttcatattt cagatgccgg aatggaaatt tgaaaccatt
      541 cgattccaga cggcgcgtcg cgatgtcggc gagaaccgat gacggccaca tttgatcgtc
      601 cgtgccggac accagcagga ccgggccgcg aatcttctgg accgggatcg tggcgcgttc
      661 gacggcgcgt tcatcgcgaa ggtgccggag atagaccggc gtgtatgccg tgggttgtcc
      721 gggccgctgc ggcggcagcg gttcgacgct gccgttgttc tcttgcagat agggcagcgg
      781 cttgccacgc cacgtccacg acgcgggggg ctgtgtatcg ccctgctcgg caaggccgat
      841 cggccagaac atgacgccgc tcggcaccca ggcgctcacg gcattgatat ccggaaaagt
      901 ggcgccgagc agcagcgcga gttcgccgcc gcgcgatggc ccccaagcgg ccagaaaacc
      961 gtcgcgcagc cagggctgcg cccgcatcca gcggatcgcg tgttcgaaat attccagcgg
     1021 aatattgacg agaccccgcg gcaggccggg ctcggcgaaa tacgcaagat tgaacgcagc
     1081 gtagccgcgc gacgcaagca tggcgccgga atactcatcc atgcctccgc cgccaccatg
     1141 taggatgaga accgagggat gtgggccctc ccccggcggc agaaacaata tgcccgctaa
     1201 tccgtcgatg cgaatgaccc tccgcgtgac accgggaccg gccgagcggc gtgtgatcgt
     1261 gagctccgcc cgctggtggt tggggccgac ggcttcaaaa tggataaacg agggttgcag
     1321 aacccagtcg tcgggaaaag gcgggctcgg aggcgacacg cgttccgcag accagatcaa
     1381 tcccatcgca gcgacgcctt cgtaactgcc cgatatcggc gcctgtcgcg cgatgccgac
     1441 cgcgccgctc tcatcggtca tgaaagtagc gtgggcctgc cagcgcgaat ggctcggaaa
     1501 gatttgagta gctgtaacgg tgaccgcctg acgcggcgtg aattccctga gttcgatggc
     1561 gacgggcaca tccatcaacg cgtcgggctg gtcaacgacg atcatgggcc accgctccga
     1621 catgccgcgg gatcaaccgc gccggttggg tttgatgccc tgccctcagg gctccaacga
     1681 tacttgcatg gaaaactcat caatgctaca attcgtagcg ctacgggacg tagtcaggtt
     1741 tcagaggctg tccgcgtcag gttgtcaccg gcgcactgtg gcttcgtgtc gttcggggca
     1801 ttgtcatgag ccgttccgca aaggctgaag cagtactggc cctccatcgg tttgggatgg
     1861 ggccgcggcc gggatcgatt gcggcgatcg aggcagaccc gcgtggggcg ctgatcgccg
     1921 aactcgaacg gccgtcggca agccagttgg cggcatccgc gttgccttcg agttcgaagg
     1981 ctttctgcac cgtcaacgag gccaatgcta agcggcgggc caaaacgatc atcgccgcga
     2041 aagcgcaaca ggaagcgaaa cgacaacaga tgtcggacgc gccctccatg atggagggca
     2101 gcgagcagga tgccaatgcg atggcggcaa agatcgccgc cgaagccgtc ccggatccgg
     2161 ggcgacagat ctatcttgac gaggcacgga cccgcaccga agccgctctc ggcaccgaga
     2221 tcggatttgt ggagcggctg gtctggttct ggtccaacca tttctgcatc tcggccgaca
     2281 agattcagag catgtccggg gcctatgagc gcgaggccat tcggccccac gttgtcggcc
     2341 ggttcgccga tatgctgctg gccgtcgagg gccatccggc gatgctgttc tatctcgaca
     2401 acaccgattc gatcgggccg aattcgatcg ccggcatcaa tcgcacccgt ggcctcaacg
     2461 aaaacattgc acgcgagatc ctggagctgc acacgctggg tgtgcgcagc ggctacagcc
     2521 aggatgacgt cttaagcttc gccaaggtgc tcaccggatg gacgctattg caaccggcca
     2581 ataatcccga gcatggcggc gaattctcgt tcaatccgcg tttgcacgag ccgggcgcgc
     2641 agaaggtgat cggaaaggtc tacgaggacg aaacggtgga acagggacgc ggcgtgcttc
     2701 gggatctggc cgcgcatccg gcgaccgcca cgcacctcgc caccaagctg gcccgctact
     2761 tcattgccga tacgcctccg cctccgctgg ttgagaggat ggcaaaggtt ttcgccgata
     2821 ccgccggcga tctcaaggag gtcgccaagg cgatggtgtc gtcggacgaa tcctggacgc
     2881 agccgcccac caagctcaag cgcccgagcg agtgggtcgt cggcatggtg cgggccgccg
     2941 gcattctgca agcggatcct gcgcggttca cggccgggca agccctgctc ggcgaaccgt
     3001 tgtggcgtcc cacgtcgccc aaaggttatc cggacgacga agcatcctgg attgacggca
     3061 tggggcggcg gctggacgtc gccaataact tcgccgagcg cgtgtcgagc cggaccgatc
     3121 cccaggaggt catcgagacc gtgctcggat cttcggtttc gcccgaggtg atgcaggcgg
     3181 tgggccgcgc cgagactcgc cagcaagccc ttgtgctgtt gttcatgtca gccgaattcc
     3241 agaggaggtg accatgacct tttgccacca cctgcccagc cgtcgcgaag cgctgatcgg
     3301 ctccggtgct ctgtttgcat ggagccaaat gcccaggctt gcgcgggcgg cgggccgcga
     3361 tccgcgcatg ctcgtcatcg tgctgagggg cgcgctcgac ggattgggtg cggtcgcacc
     3421 tgtcggcgac cccgactgga tcggcctgcg cggagatcgg gcgctggtgc tcgacggcaa
     3481 gacacccgcg ctgccgctgg atgcattttt tgcgctcaat cccgcgatgc cgaacctgca
     3541 tcgaatgtac aaggcacagc aagcgtccat cgtgcacgcc acggcgacgc catatcgaga
     3601 gcgctcgcat ttcgatggtc aggacgttct cgagagcggg atcgcccggc ccggcgcggt
     3661 cgataccggc tggctcaatc gtgccctggt cgcgctggca tcggacggtc aggtggatct
     3721 gcgcggcagc cgcgcccttg gcgttggctc ggtaacccct ctggtggtgc gcggcaaggc
     3781 ccccgtcatg tcgtgggtcc cgcagcaatt gctccccgcc agcgacgaca cgcaggcccg
     3841 cttgctggat ttgtatcagc acaccgatcc gagacttgca gtcgcgcttg aggcgcggat
     3901 acgccttgcc gcccttgggc ccgcccgagg ccccaacgat tcaatgtccg acggcgcttc
     3961 gcttccaccg cctggcattg cgcgcgtgcg tgcctatttc gccgatgccg ctggcacagc
     4021 ggcgcgcttt ctcgccaagg cggacggccc gcgtgtcggg gcggtgggct ttgtcgggtg
     4081 ggatacgcac atcagcgagg gcgcggcctc cggtcagctc gccaatctgc tcggagctct
     4141 cgacggcgcg ttggcggcca tcgagaccaa catgggcgac gcctggcgcg agacggtggt
     4201 cgcgatcatc acggagttcg gacgcaccgc gcgcatcaac ggcaccgacg gaacagacca
     4261 cggtacgggc accgttgcct ttctcgccgg cggcgcactc cggggcggac gcatggtcgc
     4321 cgactggccg ggactgaagc ccgcccaact ttatgaggga cgcgacctca aaccaaccac
     4381 cgatctgcgc gccgtactta agggtctgct cagggatcac ttgagagttg aagagagcgt
     4441 gctcgcaagc accgtgtttc ccgacagcgc cgaggtcgtg ccgaccgcgg gtcttgttgg
     4501 ctaagtcttg ttggctaaga gggaggtccg ccatgaagcc actgaccgtt atcttgtcgc
     4561 tcgcactatt cggcatcgct ctatcgtccg ggttcacaac cgcgtcggcc agcagaatgg
     4621 acggcagttg gtcccggccc taaacaggcg ttctcatgtc gattttcacc ggccgcgaac
     4681 tcgaatttct cgccagcttg aggacaagct ggcggaggct ctattggaac gagctcgcgc
     4741 gtctgctgaa agaggatgac aacgcatgcg accgacacga agcccattcc atcgcgccgc
     4801 agtccgaagg ctcgaaaggc ttcgttccga accaggcgac ctgaggatta ctccgcggcc
     4861 gcttcacgca cgtagcgcgc ggtcggcgtg cgcatggtga cgagttcttc ggccgcggtc
     4921 ggatgcagcg cgaaggtggc gtcgaaatcg gccttggtcg ccttcatctt gacggcgatg
     4981 ccgacggcct gcacgatttc ggcggcggta tcgccgacga tatggcagcc aaccacgcga
     5041 tcgctgctgc catccacgac cagcttcatc agaatccggg tatcgcggcc tgacatcgtc
     5101 gacttgatcg gcctgaaatc ggccttgtag atatcgacgt ggctgaactg cgcgcgcgcc
     5161 tgcgcttccg tcaaacccac ggtgcctact tcaggctgcg agaataccgc ggtcggaatg
     5221 ttggagtggt cgacctgaac cggatgcttg ccgaacaccg tatcggcgaa cgcgtggcct
     5281 tcgcggatcg cgaccggcgt cagattgacg cgatgggtga catccccaat cgcgtaaatg
     5341 ttcggcaccg aggtctgcga gaaaccatcg accgctatac cgccattgcc tggattgatg
     5401 gcaacgccgg cattctccag gccgagattg gcgacgttcg gatgccgtcc gatcgcaaac
     5461 atcacctgat ccgaggcgat gctggaaccg ttcgacagat gcgagatgaa ttccttgccg
     5521 ggcttgtcga ccttgttgac ggtgcagccg gtgagaatgg tgatgccttc cttctccatc
     5581 tcggcgcgca catgggcgcg gacgtcctcg tcgaagccgc gcaggatatt gtcgccgcga
     5641 taaaccagcg tcacatcgga gccgaaaccg gcaaagatgc aagcaaattc cagcgcgata
     5701 taaccgccgc cctgaatcac gatgcgcttg ggcaactccc gcagatgaaa cacttcgttc
     5761 gatgagatca cgtgttcgat gcctggaatc gccgggccat gattgggcgc gcttccggtc
     5821 gcgatcagca cgtattttgc cctgacggct tcgccattgg tcaggcgaag ggtgtgggca
     5881 tcctcgagca cggcgcgggt cttgacgatg cgcgcgccgg acttttcgac attcgtggtg
     5941 tagataccct ccagccgcgc gatctccttg tccttgttgg cgaccagcgt cggccagtcg
     6001 aaggtcaccg gcgggatgga ccatccaaag cccgcggcgt cctcaatctc gtggtggatg
     6061 tgcgatccaa taacaaacag ctttttcggc acgcagccgc ggatcacgca ggtgccgccc
     6121 atccggtact cttcggcgac catgacccgc gcgccgtagc ccgcggcgat tcgagcggcg
     6181 cgaaccccgc ccgaacctcc tccgatgacg aacagatcga cgtcgaactc agccatattc
     6241 actccgaccg aaatcgtatc tccgggatca gatttctctt accggatttc cacctagagc
     6301 ctggaatcac aaatcggtat tgcagcccat ccttcgagac ggccacttcg cggcctcctc
     6361 aggatgaggt ctcactcgtt gaaacacaaa caacctcatg ctgaggagcg agcgacgcga
     6421 gcgtctcgaa gcatgggcgg caagcgactc accgatctct tctcgtcggt agtagagccc
     6481 cgtttcgatc gaattggaac ggggctccag attcttgatt tgacgcgttt tcttgacgcg
     6541 aaccagtctc cacttcactt gaagcgctag ctagatttcc ttgccgcgtt tgcgcatctc
     6601 ggcgcgaaac tgactgttga cgacctcggc aaagttctgc gcccactggt tcatatagga
     6661 catgctggat tgaattgccc gaggctcggt cgcgagcagc ttctggccga gcgttgactt
     6721 gtagaatgta accaggtcct tcaactcctg ctcggaaaac tcgttggcat agatcttggc
     6781 cattccctcg ccgatctcct tctcacggcc ggcaaggctc t
//
LOCUS       DATNOI010000684         3022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_758916,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000684 DATNOI010000000
VERSION     DATNOI010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758916"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VN968_27390"
     CDS             complement(<1..671)
                     /locus_tag="VN968_27390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXL32991.1"
                     /translation="MLASAGFRLFANPRAARTAPYGKDGREAILSLAAFGEAKREIMS
                     WPGYKPTPLRSLPKLAARAGVGAILYKDEAERFGIGSFKALGGAYAVFKLLRKTIWEQ
                     KNIVASTLDLTSGRYADLTSHITVTCATDGNHGRSVAWGAHTFGCRCVIYIPETVSEG
                     RCQAIAAYGAEVKRVAGTYDDGVRRVAADAAVQGWTVVSDTSYEGYTDVPRDVMQGYS
                     LMVDEA"
     gene            complement(680..2278)
                     /gene="ggt"
                     /locus_tag="VN968_27395"
     CDS             complement(680..2278)
                     /gene="ggt"
                     /locus_tag="VN968_27395"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXL32992.1"
                     /translation="MPFCSGTAHDFELPGRSEAFGMRVMAATSHPLATLTALDVLRRG
                     GNAMDAAVAAIALLGVVEATQTGIGGDCFVLYMRRGEGEIIALNGSGWAPAAASLDRY
                     VSRGMRWIPSDSADAVTVPGAVASWARLIDDHGTMKLADLLQPAIAAAEDGYPVTERV
                     ARDWARQADKLRKNKTAASIFLPNGATPRPGDVHRQPALAAALRSIASDGPTAFYEGW
                     MVDALRAAGGCHTLEDFSTYAPEYVTPIGTSYRGYEVWECPPNGQGVVPLAMLKALEG
                     FDASAWAPLSVERCQALTEIGRQAYADRDCFVGDPRTGEVPLEALLSDERSAKLRGRV
                     SMERRMDGLAPFPIPEHCDTAFLTVVDADRNTVAFINSIFDDFGTGIVSPAAGVIFHN
                     RASGFVLEPEHPNAIAGRKRPLNTIIPAMVTRKGKAVMPFGVTGGQFQPFGQVEVLTN
                     IIDYGMGIQAAIDAPRIFPLGDTLQVESTVSPAVVAGLSMLGHHVVRAENPLGTAQAI
                     WIDWEAGLLRGGADGRRDGLAAGW"
     gene            complement(2265..>3022)
                     /locus_tag="VN968_27400"
     CDS             complement(2265..>3022)
                     /locus_tag="VN968_27400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HXL32993.1"
                     /translation="RQAIRGGAYTGYTAGIAPGFVQANICILPAKWAEDFLLYCQRNP
                     RPCPLIARSDVGDPRLPTLSDDLDIRTDVPRYHIFRDGTFVEEVTDIRSYWRDDLVTF
                     ALGCSFSFEEALQEAGVRLKFLERNNVAAVYVTNVDTVPAGPFKGPLVVTMRAFTPAD
                     AIRAVQITSRFPNVHGAPVHLGNPGSIGVDLSRRYLDVGDPEVADDELPVFWACGLTP
                     QLAVKNARPPLCITHAPSSMLITDLRNASLAVL"
BASE COUNT          566 a          987 c          971 g          498 t
ORIGIN      
        1 gcttcgtcga ccatcaggct gtaaccctgc atcacatcgc gcggaacgtc cgtgtaacct
       61 tcgtaagacg tatcggagac cacggtccac ccctgcaccg ccgcatccgc ggcgacgcgc
      121 cgaacgccat cgtcataggt cccggccacg cgcttgacct cggcgccata ggcggcgatt
      181 gcttggcaac ggccttcgct gacagtttca gggatataga tcacgcatcg gcagccgaag
      241 gtgtgagcgc cccaggcgac cgaacgccca tgattgccat cggtcgcgca agtcaccgtg
      301 atgtgcgagg tcagatcagc gtaacgcccc gacgttaggt cgagcgtcga tgcgacgatg
      361 ttcttctgct cccagatcgt cttgcgtagc agcttgaata ccgcgtaggc gccacccagc
      421 gccttgaagc tgccaatgcc gaagcgctcc gcttcgtcct tgtagagaat cgcaccgacg
      481 ccggcgcgcg ccgcgagctt gggaagggag cgcaaaggcg tcggcttgta accgggccac
      541 gacatgatct cgcgcttggc ttcgccaaag gccgcaagac tgaggatcgc ctcgcgcccg
      601 tccttgccat agggcgccgt gcgcgccgcc cgcggattcg caaataggcg gaatcccgcg
      661 gaggccagca tctcagtcct caccacccgg ctgccaagcc gtcacgacgg ccatccgcgc
      721 cgccgcgaag gaggcccgcc tcccaatcga tccagatcgc ctgcgcggtg ccgagcggat
      781 tctcggcgcg cacgacatga tggccaagca tcgaaagtcc cgccacgacg gctggagaca
      841 cggtgctctc gacctggagg gtatcgccca gggggaaaat ccgcggcgcg tcgatcgctg
      901 cctgaattcc catcccgtag tcgatgatat tggtcaggac ctcgacctga ccgaagggct
      961 gaaactgccc gccggtcacg ccgaacggca tcaccgcttt tcccttccgc gtgaccatgg
     1021 cgggaatgat cgtgttcaac ggccgcttgc gtccggcaat cgcattggga tgttcgggct
     1081 cgagcacgaa gccggaggcg cggttgtgga agatgacccc ggccgcgggg ctgacgatgc
     1141 cggtcccgaa atcatcgaag atcgaattga tgaacgccac cgtgttgcga tcggcgtcga
     1201 cgacggtgag aaaagccgtg tcgcagtgtt ccggaatggg gaagggtgcc agaccgtcca
     1261 tgcgccgctc catcgacaca cgtccacgca gcttggccga gcgctcgtcc gacagcaggg
     1321 cctcgagcgg gacctcgccg gtgcgcggat cgccaacgaa gcaatctcga tcggcgtagg
     1381 cttgccgccc gatctcggtc agcgcttgac agcgttcgac cgaaagtggc gcccatgcgg
     1441 aggcatcgaa accttcgagc gccttcaaca tggcgagcgg gacgacgccc tggccatttg
     1501 gcggacattc ccaaacctca taaccgcggt agctggttcc gatcggtgtc acgtattccg
     1561 gtgcataggt agaaaaatct tccagcgtgt gacagccgcc ggcggcgcgc agcgcatcga
     1621 ccatccagcc ttcgtagaag gctgtcggtc catcgctggc gatgctgcgc agcgcggcgg
     1681 cgagcgccgg ctggcgatgg acatcgcccg gccgcggggt cgcgccattc ggcaagaaaa
     1741 tgctggccgc agtcttgttc ttgcgcaatt tgtccgcctg ccgcgcccag tcgcgtgcga
     1801 cccgttcggt caccggatag ccgtcttcgg ccgccgcgat agcgggctga agcaagtccg
     1861 cgagcttcat cgttccgtga tcgtcgatga gccgcgccca tgacgcgacc gcacccggca
     1921 cagtcaccgc gtcggcgctg tcggacggga tccatctcat tcctcggctc acataccgat
     1981 cgagacttgc cgccgcgggc gcccagcccg aaccgttgag cgcgatgatc tctccctcgc
     2041 cgcgacgcat gtagaggacg aagcaatcgc cgccaatgcc cgtctgcgtc gcttcgacga
     2101 cgccaagcag cgcgatggcc gcgacggcgg cgtccatcgc attgccgccg cgacgaagca
     2161 cgtcgagcgc ggtcagcgtt gccagcgggt gcgaggttgc cgccatgacg cgcataccga
     2221 acgcctcgga acggccgggc agctcgaaat cgtgggcggt gccactacag aacggcaaga
     2281 gacgcgttcc gcaaatccgt gatcagcatc gaggacggcg cgtgggtgat gcacagcggc
     2341 ggtctcgcat tcttcacggc cagctgcgga gtaagtccgc aggcccagaa caccggcaac
     2401 tcgtcgtcgg cgacctccgg gtcgccgaca tccaaatagc gccggctcaa atccacgccg
     2461 atgctcccgg ggttgccgag atggactgga gcgccgtgaa cgttcgggaa acgcgatgtg
     2521 atctgaacag cgcggatggc atcggctggc gtgaaggccc tcatggtgac caccagcggg
     2581 cccttgaacg gccccgctgg gacggtgtcg acattggtca cgtacacagc cgcgacattg
     2641 ttgcgctcga ggaatttcag ccgcacgccg gcctcctgta gggcttcctc gaacgaaaac
     2701 gagcagccga gggcgaaggt gacgaggtca tcgcgccagt acgagcggat gtcggtcact
     2761 tcctcgacga atgtgccgtc gcggaagatg tgatagcgcg gaacatcggt gcggatatcc
     2821 agatcgtcgg acagcgtcgg caggcggggg tcgcccacat cggagcgtgc gatcaacggg
     2881 cacggcctgg ggttgcgctg acaataaagg agaaaatcct cggcccattt ggcaggaaga
     2941 atgcagatat tggcctgtac gaagcccggc gcgatgccgg cggtgtagcc ggtatacgcg
     3001 cccccgcgga tcgcttgccg tg
//
LOCUS       DATNOI010000685         7471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1524496,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000685 DATNOI010000000
VERSION     DATNOI010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524496"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="VN968_27405"
     CDS             complement(<1..1183)
                     /locus_tag="VN968_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXL32994.1"
                     /translation="MAYKNFKIETDADGIALVTWDIPGRSMNVLDETTMSELEEIVKQ
                     TSADAAVKGVVVTSGKEAFSAGADLSMLEGMNRRYTELLKAKGDVAANQMLFDESRKS
                     SLVFRAIETSGKPWAAAINGLALGGAFELTLSCHYRVAAENPKTRLGLPEVKVGLFPG
                     AGGTQRVPRIVPPQDAMQLLLKGEAVNLEKAKALKLVDAVVPAGDLIKAAKDWIKGGG
                     KAVAPWDEKGFKLPGGPVFSKAGMMMFPAGNAIFRRETYDNYPAARAIMQCVYEGLQL
                     PIDAALRVESRYFTQILRSKEAAAMIRSLFLSMQELNKGARRPQHVPPTKVKKLAIIG
                     AGFMGASVGYVSARAGIEVVLIDRDQEAADKGKAYSANLMAGQIKRGRAKQEDADKLL
                     AR"
     gene            complement(1195..1671)
                     /locus_tag="VN968_27410"
     CDS             complement(1195..1671)
                     /locus_tag="VN968_27410"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL32995.1"
                     /translation="MPKIDLSALPARSGTTYPEPFAGIVDGREWQSLGAAAGLSQFGV
                     NLVRLKPGAASSARHWHEQEDEFVYMIEGELVLVEDEGETIVKPGDAAGFKAGVPNGH
                     HLLNRTDRDAVFLVVGTRAPRERCHYPDIDLAFEQDGDRYRTTHKSGAPYGDWQHD"
     gene            complement(1805..3013)
                     /locus_tag="VN968_27415"
     CDS             complement(1805..3013)
                     /locus_tag="VN968_27415"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXL32996.1"
                     /translation="MPEAYIYDHVRTPRGRGKADGALHEVTALALATAPLKALKERNN
                     LAQDVVDDVILGVVDPVGEAGSDIARFAALNAGLGESVPGIQISRFCASGLDAVNFAA
                     AQIMSGQHELTIGGGAESMSRVGIGASGGAWPMDPSIAVPAYFMPQGVSADLIATKYG
                     FSRDDVDAYAVQSQQRAAKAWDEGRFNNSVIPVKDVNGLTILAKDEHMRPTTTMQSLG
                     QLQPSFVAMGQMGGFDAVAIQSHPEIERINYVHHAGNSSGIVDGAGAVLLGSKEAGAK
                     HGLKPRAKIRAFANIGSEPAMMLTGPVDVTEKLFERSGMKKSDIDLFELNEAFASVVL
                     RYIQAFDIDNAKINVNGGAIALGHPLGATGAMILGTVLDELERTNKSTALITLCIGGG
                     MGTATIIERV"
     gene            complement(3102..4892)
                     /locus_tag="VN968_27420"
     CDS             complement(3102..4892)
                     /locus_tag="VN968_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HXL32997.1"
                     /translation="MPIYKAPLEDVNFLLNDVFQIDRYDNLPGFSDASADVREAILNE
                     AAKLSEEVLQPLNRVGDIEGCTRHDDGRVTTPNGFKEAYKQVAEGGWLGLSAPTEYGG
                     QGLPVTLSQTVTEFQSSANMAFSMYGGLTMGATAALIVHGKPEQKTMFVPKMVAGQWT
                     GTMNLTEPQCGTDLGLLRTKAVKQADGSYKITGTKIFISAGEHDLAENIIHLVLARIE
                     GAPAGIKGVSLFVVPKILVNADGSLGARNGVSCGSIEHKMGIHGNSTCVMNYDGATGW
                     LIGEENKGMQGMFVMMNEARLGVAVQGLAQSEVAYQNAVAYARDRLQGRSLSGAKAPD
                     KPADPIIVHPDVRRTLLTIRAFNEAARAMVVWTALKSDVAHRSSDPKDRQEADDHMGL
                     MTPVMKGVMTDVGFSNAVLAQQMYGGHGYIAEQGMEQFVRDARIAMLYEGANGIQALD
                     LVGRKLPRDGGRAVMSFFGEVAGFAKDHGADEAMKPYVAPLSAALGHLQQATTWLMQN
                     AMAKPDNAGAAATDYMQLFGLVTFAYMWARMAKVAQDKIAAGGTTPYLTTKLVTGRFF
                     MERMLPETSVHVARIQTGSATTMELAAEAF"
     gene            5227..>7471
                     /locus_tag="VN968_27425"
     CDS             5227..>7471
                     /locus_tag="VN968_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL32998.1"
                     /translation="MNSRLSWSVEGIDPSVRERAEAAARRAGMSLSDWLNSTIGDFAL
                     PNFRATLGQPTAQPGREGPDVADIHQRLDAITRQIEKIARPAPRSDAPRNDAARSEPA
                     VARQLNDAISRLDARLSQISDPTPARQNKLQDEQRQADLVERAAAQVYRASPPLSPAS
                     LDVAIAEIAARQSELDNPMPRPLPPRSAPPVVPATPTGPDFSALERHLVKITSQIEAL
                     QRPDGIEQSIATFRGELTEIRHAITEAMPRRAIESIENEILSLSRRIDDTRQSGTDGE
                     ALAGVERALGEIREVLGSLKPAEQLAGYDEAIRNLGTKLDQILRANDDPSTVRQLENA
                     IAALRSIVSNVASNDALVRLSNDLQTLSSKVDQLARSESQSDSFAILEQRIAALTSTL
                     ESREPPVVSESSEHLEIALRALSDRLDGMPVGNYNASAFAHLEQRVSYLLERLEASTD
                     HHTGNLGRVEDGLHDILRHLENQSASFAALTESSRRPAEPVDSGLIDVVKRELSDIRF
                     SQSETGRHTQDSLEVVHNTLGHVVDRLAMIEGDLRSVRVAPAAPQSETPPAPQPVPAQ
                     PATPLAAIPPQPKPELPNPAAAQAHFATAPREFQAVQAPLPAIAAATPRAIGEILESH
                     AAQPQAAIVLELPPDHPLEPGTRPSGRVPSPSERIAASESAISEITASAREQTSASNF
                     IAAARRAAQAAAAAPVGDKAARSPFKDPARDKTKETSAVTSKIRALLVGASVVVIVLS
                     TFKMAMTL"
BASE COUNT         1316 a         2524 c         2279 g         1352 t
ORIGIN      
        1 tgcgcgcaag cagcttgtcg gcatcctcct gcttggcgcg gccgcgcttg atctgccccg
       61 ccatcaggtt tgccgaatag gccttgccct tgtcggccgc ttcctgatcg cggtcgatca
      121 gcaccacctc gatgccggcg cgtgccgaaa cgtagccgac gctggcgccc atgaagccgg
      181 cgccgatgat ggctagcttc ttgaccttgg tcggcggcac atgttgcggc cgccgcgcgc
      241 ccttgttcaa ttcctgcatc gacaggaaca ggctgcggat catcgccgcg gcttccttgc
      301 tgcgcaggat ctgggtgaag tagcgcgatt cgacccgtaa agccgcgtcg atcggcagct
      361 gcaatccttc ataaacgcac tgcatgatgg cgcgcgccgc cggatagtta tcgtaggtct
      421 cgcggcggaa gatcgcgttg ccggccggga acatcatcat gccggccttg gagaacaccg
      481 ggccgcccgg cagcttgaag cctttctcat cccagggcgc gaccgccttg ccgccgccct
      541 tgatccagtc ctttgcggcc ttgatgagat cgccagccgg gacgacagca tcgaccagct
      601 tcaacgcctt ggctttttcg agattgaccg cttcgccctt cagcagcaac tgcatcgcat
      661 cctgcggagg aacgatacgc ggcacgcgct gggtgccgcc cgctccggga aacagcccga
      721 ccttgacctc cggcaacccg agacgggtct tcggattctc ggcggcgacg cggtaatggc
      781 acgacagcgt gagttcgaac gcgccgccga gtgcgaggcc gttgatggcg gcagcccacg
      841 gcttgccgct ggtctcgatg gcgcgaaaca ccagcgagga tttgcggctc tcgtcgaaca
      901 gcatctggtt ggccgcgacg tcacccttgg ccttgagcaa ttcggtatat cggcggttca
      961 tgccttcgag catcgacaga tcggcgccgg cggagaacgc ctccttgccc gaggtgacga
     1021 cgacgccctt gacggcagcg tcggcggacg tctgcttcac gatctcttcg agctcggaca
     1081 tcgtggtttc gtcgagcacg ttcatcgaac ggccgggaat gtcccacgtc accagcgcaa
     1141 tgccgtcggc gtcggtctca atcttgaaat tcttgtaagc catagcgtgc tccctcagtc
     1201 gtgttgccag tcgccatacg gcgcgccgga tttatgcgtt gttcggtaac gatctccgtc
     1261 ctgctcgaaa gcaagatcga tatcggggta gtgacagcgt tcgcgtggcg cgcgcgtgcc
     1321 gacgacgaga aacacggcgt cgcggtcggt gcggttcaaa agatgatggc cgttgggaac
     1381 accggccttg aagcccgcgg cgtcgcccgg cttcacgatc gtctctccct catcctcgac
     1441 cagcaccagt tcgccttcaa tcatataaac aaactcgtct tcctgctcgt gccaatggcg
     1501 cgccgacgat gctgcccccg gtttcaaccg cacgaggttg acgccgaatt ggctcaagcc
     1561 cgccgccgca cccagcgatt gccattcgcg gccatcgaca attccagcga acggttccgg
     1621 atacgtcgtt ccactgcgcg ccggcaatgc actcaggtcg atcttcggca tcagtccctg
     1681 ttctcgcctc agttcgtagg gtgggcaaag gcgcactcgc gccgtgccca ccattcttat
     1741 ccggcgacgg tttcggtggg cacgcttcgc tttgcccacc ctacgatcgc tacgatcgct
     1801 cgccttaaac ccgctcgatg atcgtcgcgg tgcccatgcc gccgccgatg cacagcgtga
     1861 tcagcgcggt cgatttgttg gtgcgctcga gttcgtcgag cacggtgccg agaatcatgg
     1921 caccggtggc gccgagcgga tggccgagcg cgatcgcgcc gccattgacg ttgatcttgg
     1981 cgttgtcaat gtcgaaggcc tggatgtagc gcagcaccac cgaggcaaac gcctcgttca
     2041 attcgaacag atcgatgtcc gatttcttca tgccggagcg ctcgaacagc ttctcggtga
     2101 cgtcgaccgg gccggtcagc atcatcgccg gctcagagcc gatattggcg aaggcgcgga
     2161 tcttggctcg cggcttcagg ccgtgttttg caccggcctc cttgctgccg agcaggacgg
     2221 cgccggcacc gtcgacgatg cccgacgagt tgcccgcatg atgcacgtag ttgatgcgtt
     2281 cgatttccgg atgcgactgg atcgccaccg catcgaagcc gcccatctgc cccatcgcca
     2341 cgaaggacgg ctgcagctgg ccgagcgact gcatcgtcgt cgtcggccgc atgtgctcgt
     2401 ccttggcgag aatggtgagg ccattgacgt ccttcaccgg gatcaccgaa ttgttgaagc
     2461 ggccctcgtc ccacgccttg gcggcgcgtt gctggctctg caccgcatag gcgtcgacgt
     2521 cgtcgcgtga gaaaccgtat ttggtcgcga tcagatcggc cgacacgccc tgcggcatga
     2581 aataggccgg cacagcgatc gacgggtcca tcggccaggc gccgccggag gcgccgatcc
     2641 cgacccggct catcgactcg gcgccgccgc cgatggtgag ttcgtgttga ccgctcatga
     2701 tctgggcggc cgcgaaattc acggcatcga gacccgaggc acagaagcgg ctgatctgaa
     2761 tgccgggaac ggattcgccg agaccggcgt tcagcgccgc gaagcgcgcg atgtcggagc
     2821 cggcctcgcc gaccggatcg accacgccga gaatgacgtc atcgacgacg tcttgggcca
     2881 gattgttgcg ctccttgaga gccttcagcg gcgccgtcgc cagcgccagt gcggtcactt
     2941 catgcagcgc gccatcggcc ttgccgcggc cgcgcggcgt gcgaacgtga tcgtagatat
     3001 atgcctcagg catgacgccc tcctggtatg aggatcataa tatataggcg attgggaagc
     3061 cgtcgcggat ctggtcgaac gccgtggaag aattgctccg atcagaacgc ttccgccgcc
     3121 aattccatgg tggtcgcgct tccggtctgg atgcgcgcaa catgcaccga tgtttccggc
     3181 agcatccgtt ccatgaagaa gcggccggtc accagcttgg tagtcaaata gggcgtcgtg
     3241 ccgccggccg caatcttgtc ttgcgcgact ttcgccatcc gcgcccacat gtaggcgaaa
     3301 gtgacgaggc cgaacagctg catgtagtcg gtggcggcgg cgccggcatt gtccggcttg
     3361 gccatcgcgt tctgcatcag ccatgtggtg gcctgctgca gatggccgag cgccgcgctc
     3421 agcggcgcga catagggctt catggcctcg tcggcgccgt gatccttggc gaacccggcc
     3481 acttcgccga aaaacgacat caccgcgcgg ccgccatcgc gcggcagctt gcgcccgacc
     3541 aggtcgagcg cctgaatgcc gttggcgccc tcatagagca tggcgatgcg cgcgtcgcgc
     3601 acgaactgct ccatgccctg ttcggcgata tagccgtggc cgccgtacat ctgctgcgcc
     3661 agcaccgcgt tggaaaatcc gacgtcggtc atgacccctt tcatgaccgg cgtcatcaat
     3721 cccatgtgat cgtccgcttc ctgacggtcc ttgggatcgc tggaccggtg ggccacgtcg
     3781 cttttgagcg cggtccacac caccatcgcg cgcgccgcct cgttgaaggc gcgaatggtg
     3841 agcagcgtgc ggcgcacgtc gggatgcacg atgatcggat cggccggctt gtccggcgcc
     3901 ttcgcgcccg acagcgagcg gccctgcaga cgatcgcggg cgtaggcgac ggcgttctga
     3961 taggcgactt cggattgcgc cagcccctgc accgcaacac cgagccgcgc ctcgttcatc
     4021 atcacgaaca tgccctgcat gcccttgttt tcctcgccga tcagccagcc ggtggcgccg
     4081 tcgtaattca tcacgcaggt ggaattgccg tgaatgccca tcttgtgctc gatcgaacca
     4141 caagacacgc cgttgcgcgc gcccagcgat ccatcggcat tgaccaggat cttcggcacc
     4201 acgaacagcg acacgccctt gatgccggcc ggcgcgccct cgatccgcgc cagcaccaga
     4261 tggatgatgt tttccgcaag atcgtgctcg ccggcggaga tgaaaatctt ggttccggta
     4321 atcttgtagc tgccgtcggc ctgcttcacg gccttggtgc gcagcaggcc gagatcggtg
     4381 ccgcattgcg gctcggtcag gttcatggtg ccggtccatt gtccggccac catcttcggc
     4441 acgaacatgg tcttctgctc gggcttgccg tgcacgatca gcgccgcggt ggcgcccatg
     4501 gtcaggccgc catacatcga aaacgccata ttcgccgagc tctggaactc ggtgacggtc
     4561 tggctcaacg tgaccggaag tccctgcccg ccatattccg tcggcgccga caggccgagc
     4621 cagccgcctt cggccacctg cttgtaagct tccttgaagc cgttcggcgt cgtcacccgg
     4681 ccgtcgtcgt ggcgggtgca accctcgata tcgccaacgc gattgagcgg ctgcaagact
     4741 tcttcgctga gtttcgcagc ctcattgagg atggcctcgc gcacgtccgc ggacgcgtcg
     4801 ctaaagccag gcagattgtc gtagcggtcg atctgaaaca cgtcgttgag caggaaattg
     4861 acatcttcca ggggggcttt gtagatcggc atggtgtcct ctcggctggc tggcgggcct
     4921 catttaggga aaattgtgca gcgcagcaat cgtccggatc cggaagtgga tcttagacgg
     4981 ttcctgacgc ccaaggcagc tatccagtca gccattgcga caacgtgacc ggcgcgaccg
     5041 ggaccgccgt tcgcccaacc cggtcaattt taccgatagc gtaactttca ccgcctgctg
     5101 actgattctt taacccgttc tttaccgtaa cacgaaaaag tcgatgccag aggcagacgg
     5161 cctgcgcgga attcgattgt ctccacacca gggacgcgcg ggacggattc ggaaccggac
     5221 gaaagcatga attcgcgcct atcgtggagt gttgagggca tcgatccatc cgtccgggag
     5281 cgggccgagg cagccgcgcg ccgcgccggc atgtcgctca gcgactggct caattccacc
     5341 atcggtgatt tcgctctgcc aaactttcgc gcaaccctcg gtcaaccgac ggcacagccg
     5401 ggccgggaag gccctgatgt cgccgacatt catcagcggc tggatgccat tacccgccag
     5461 atcgagaaga tcgcacgccc ggcgccgcgc agcgacgcgc cccgcaacga cgcggcgcgc
     5521 agcgagccgg ccgtcgccag acaattgaac gacgccatct cgcgcctcga cgcgcggctg
     5581 tcgcagatct ccgatcccac gccggcgaga caaaacaagc ttcaggacga gcagcgccag
     5641 gccgacctgg tcgagcgcgc ggcggcccag gtctatcgcg cttctcctcc cctcagcccg
     5701 gcgtcgctcg acgtcgcgat cgcagagatc gccgcgcgcc agagcgaact tgacaatccc
     5761 atgccgcgac ccctgccgcc gcgcagcgca ccgccggtgg tgcccgcaac gccgacgggg
     5821 ccggattttt ccgcgctcga gcgccatctc gtcaagatca ccagccagat cgaagccttg
     5881 cagcgtccgg acgggatcga gcaatcgatc gccacgttcc gcggcgaatt gacggaaatc
     5941 cgtcacgcca ttaccgaggc gatgccgcgc cgggcgatcg aatcgatcga aaacgaaatc
     6001 ctctcgctgt cccgccgcat cgacgacacc cgccagagcg gcaccgacgg cgaggcgctg
     6061 gccggcgtcg aacgcgcgct gggcgaaatt cgcgaagtgc tgggctcgtt gaaacccgcg
     6121 gagcaactgg cgggttacga cgaggcgatc cgcaatctcg gcaccaagct cgaccagatc
     6181 ttgcgcgcca acgacgatcc ctcgacggtc cgccagctcg aaaacgccat cgcggcactt
     6241 cgctccatcg tctccaatgt cgcgtccaac gacgcgctgg tccggctcag caacgacttg
     6301 cagacattgt cgtccaaggt cgatcagctt gcccgctccg aaagccaaag cgattcgttc
     6361 gcgatcctcg agcagcgcat cgccgcgctg acctcgacgc tggaaagccg tgagccgccg
     6421 gtggtcagcg aaagctccga gcatctcgaa attgcgttgc gggcgctgtc cgaccgcctc
     6481 gacggtatgc cggtcggcaa ctataacgca tccgccttcg cccatctcga acagcgcgtg
     6541 tcttatctgc tggagcgcct cgaagcgtcc accgatcacc acaccggcaa tctcggacgg
     6601 gtcgaggacg ggttgcatga catcctgcgc catctcgaaa accagagtgc gagctttgcc
     6661 gccctgaccg agagcagccg ccgccctgcc gaaccggtgg acagcggcct gatcgatgtc
     6721 gtcaagcgcg aattgtccga catccggttc agccagtcgg aaaccggccg ccacacccag
     6781 gattcgctcg aagtcgtcca caacacgctc ggccatgtgg tcgaccggct ggcgatgatc
     6841 gaaggcgatc tgcgctcggt tcgcgtcgcg ccggccgcgc cgcagtcgga aacgccgcca
     6901 gcccctcagc ccgttccggc gcaaccggca acacctctgg ctgcgatacc gccgcaacca
     6961 aagcctgaat tgccgaatcc ggcagcagca caggcacatt tcgccaccgc cccgcgggaa
     7021 ttccaggccg tgcaggcacc gctgccggcg atcgctgccg cgacgccaag ggcgatcggc
     7081 gagattctgg agtcgcatgc ggcgcagccg caagccgcga ttgtgctcga gctgccgccc
     7141 gatcacccgc tcgaacccgg gacacggcct tccgggcgcg taccttcgcc gtcggaacgc
     7201 attgccgctt cagaaagcgc gatcagcgaa atcacggcga gcgcgcgcga gcagaccagc
     7261 gcatcgaatt tcatcgccgc cgcgcgtcgt gccgcccagg ccgccgcggc cgcgccggtg
     7321 ggcgacaagg ccgcccgcag cccgttcaag gatcccgccc gcgacaagac caaggaaaca
     7381 tcggcggtca cctccaagat tcgcgcgctg ctggtcggcg cgagcgtggt cgtgattgtg
     7441 ctctccactt tcaagatggc gatgacgctg c
//
LOCUS       DATNOI010000686        17140 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2410945,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000686 DATNOI010000000
VERSION     DATNOI010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17140
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2410945"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VN968_27430"
     CDS             complement(<1..319)
                     /locus_tag="VN968_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HXL32999.1"
                     /translation="MKNIIQNNRLIGAGLVLAVVAMAGGATFLNGRIGAAQAETVAVA
                     PAALPVSVSVVTPRETVPWDEFSGRLEAVERVEVRSRVAGAIQEIHFREGALVKQGDL
                     LILI"
     gene            509..1117
                     /locus_tag="VN968_27435"
     CDS             509..1117
                     /locus_tag="VN968_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL33000.1"
                     /translation="MAMGRPREFEVDKALDVALQVFWRKGYEGASMADLTEAMGITKP
                     SLYAAFGNKEELFRKALDRYVDGPGGYFQVALGMPTARTVVEHLLYEAADAVTDPNNP
                     PGCLAVQGALCCGDAAQSIKQELMLRRANGEEDLRKRFDRAIAEGDLPAGSDAADLAR
                     YISAILQGMAVQAAGGTNRDQLRKIAEMALRTWPPTASISPT"
     gene            complement(1157..1735)
                     /locus_tag="VN968_27440"
     CDS             complement(1157..1735)
                     /locus_tag="VN968_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HXL33001.1"
                     /translation="MRYDKGHKDATRQRIIDVASRQFRERGVAAVGLAGIMTDAGLTN
                     GAFYAHFASKEDLLREVLCNARKRNEMWQAATESGAGLVGTIRDYLSLGHRDNPGRGC
                     PTSALVAEIARHPKAARDAFTDKLVGIIELIAARLDSGTQRERRRKAVALYGLMVGTL
                     QFARAVNDKRLSEEILQSGAEQALALVGQRGK"
     gene            1854..3371
                     /locus_tag="VN968_27445"
     CDS             1854..3371
                     /locus_tag="VN968_27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXL33002.1"
                     /translation="MSLQKPVPSDGVLIGSPSEPTAEAPAPVAAAEVEAIGRISVVET
                     PAVPAAPDHPTKAMQTRSAILTGPVLPTLLRLALPTMTVLLAQTAVNVAEAYYVGFLG
                     TQALAGVALVFPVFMLMMMMSNGGLGSGVASAVARATGAGHKHDADALVLHAVVLAVI
                     VGALFTIGAASGGPALYQALGGQDETLNAALKYSNYLFAGAIPVWIVNFLAAALRGSG
                     NVRVPAMVTLAGALVLIPFSPILIFGFGPIPRLGIAGAGIAFALYYGAAMLVLLRYMT
                     TGRSGLTLKSGSLEGRLFKDILRVGLPTAFSTVLTNLTVIVVTGAVGLFGTTALAAYG
                     IASRLDYVMIPILFGICTSVLTMVGVNMGAGQVARAKRIARISSFVGAGIAGSIGLIV
                     AIFPMLWLRLFSHDPDVLAVGSTYLRIVAPAYGALGFGFVIAFAAQGAGHVLWPFVGA
                     TSRLVLAAGCGWIAVSYLGGGMATLAAMVSTSLVGYAAICAIAMFSGAVWRAQKG"
     gene            complement(3393..4334)
                     /locus_tag="VN968_27450"
     CDS             complement(3393..4334)
                     /locus_tag="VN968_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL33003.1"
                     /translation="MKRIIFAAGLLLGAMASATAQTTLRVGDQKGNSQAVMEAAGVLK
                     DVPYKVEWKEFPAAAPLLEALGAGAIETGLVGDAPFTFAAAANVPVRAIGAIRQSQEG
                     LAVLVPRESHILSFEDLRGKKIATGRGSIGHQLILAALESRGWSLPDVQIVFLAPSDA
                     KVAYTQGSVDAWSTWEPYVSQEEVLFQSRRVITAEGLTAGIGFQVASSNAIRDKRPEL
                     EDFLRRLTAARAWSLTNVSAYAETWGKLMNIPPAVPLNWLSRAKIRIAPIDDGVVADE
                     QKTIDLYFRSGLIKQQLNAADIVDRSFSHAIGKGAGL"
     gene            complement(4331..5431)
                     /locus_tag="VN968_27455"
     CDS             complement(4331..5431)
                     /locus_tag="VN968_27455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXL33004.1"
                     /translation="MSVEFIGFITNNNSSETIVRTGPVLDRRYIETVAKAHETAGFDR
                     ALLAFHSTSPDCLQVGQHVLGVTDRLKVLIAQRPGFTAPTLIARQLATVDQLSGGRVA
                     LHVITGGNATELKQDGNTIDDKDERYARTDEFLDVVRLEWTSEKPFSYNGKYYQVQNG
                     FSQVKPFRSEGIPIFVGGASDAAIEVAGKHADTFALWGESYAQVRDVTSRVQVAAATH
                     GRPTPRFSLSVRPILADTEEKAWAKAEEILARATALQDKTGYRKPADGHATAGARRLL
                     ALAEQGTRIDKRLWTEMARLTGANSNTTALVGTPEQVAEVFGDYYDLGVSHFLIRGFD
                     PLIDAIEYGRELIPLTRKLIAERRLAKGVAAE"
     gene            complement(5469..6653)
                     /locus_tag="VN968_27460"
     CDS             complement(5469..6653)
                     /locus_tag="VN968_27460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL33005.1"
                     /translation="MVSAPGNSIRKEVFTDATPAIFDCAKLLASEFAATAALHDREAS
                     FPFENFNQLSEAGLLALTVPTALGGSGAGALDAARIINIFGKADPSTALVLSMHYIQH
                     LVMTRSARWPGRLARKLARETVEGVALINALRVEPDLGSPSRGGLPATMARRTETGWR
                     LSGRKIYSTGAPILKWYAVWARTDEPTVRVGLFLVPAGLPGTRIIETWDHLGLRASGS
                     HDVVFDDVLFPLDHEIDVRKPDEWLTPDFTQATVHAIFVAAIYDGVARAARDWLIEFL
                     KSRVPSNLGASLATLPRMQEVVGGIEARLAVNARLIESFAGDYDDGVLLSASESNIVK
                     LAVTNNAVAVVEDALSLTSNHGLTRANPLERYYRDVLCGRVHTPQDDATRINAGRLAL
                     GI"
     gene            complement(6647..7444)
                     /locus_tag="VN968_27465"
     CDS             complement(6647..7444)
                     /locus_tag="VN968_27465"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL33006.1"
                     /translation="MVALAPAERAAPAVAGASLDVEAVSHAFDIDGAVLPVLDDISFS
                     VKPGEFVALLGPSGCGKSTLLRLVAGLEPPRSGGLREDGEPITGPFPSRVVVFQDPTL
                     FPWRKVWDNVALGLEAQGILKHQRHRVDAAIDLVGLSTFCNAYPHQLSGGMAQRVALA
                     RALVNDPKILILDEPLGKLDSLTRITMQAEIASLWQRSGFTTLLVTHDVEEAVFLANR
                     VIVFSDRPARVKADIAVDRPYPRHRGDPKLAVLRRNILGLLGLDATW"
     gene            complement(7438..8478)
                     /locus_tag="VN968_27470"
     CDS             complement(7438..8478)
                     /locus_tag="VN968_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXL33007.1"
                     /translation="MSMYSASKTVAFPQSAEEGAAFAGRPLSLPTWFLASGTALFATA
                     AWIGFGLSCLLWKDLGDWSRTDALAIAAFIIAGGILAATASGKWLGRFGTGLQARAPW
                     LLAFGLFLTLWEIATAKFAWLPLPFFPPPQAIIEVYTDDLPKLLDSVFASVKLQLGGY
                     AIGAAAGFLTGVSIGWSRSVGYWVHPVLRFIGPLPATAWLPFAFFTFPSSWSASTFLI
                     ALATGFPVTVLTWSGVASVSNAYYDVARTLGAKPSFLVLKVAIPAALPHVFVGLFMGL
                     GSSFAVLVVAEMIGVKAGLGWYLQWAQGWAAYSNMYAALIVMSLLCSGAITLLFRTRD
                     RLLVWQKGVVKW"
     gene            complement(8456..9556)
                     /locus_tag="VN968_27475"
     CDS             complement(8456..9556)
                     /locus_tag="VN968_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL33008.1"
                     /translation="MSRDMKWGNALLDRRTLLRAGAATAFAAPLGVFGAQAFPFRAAT
                     PNIDFSEFPICKASSEAVPLAGAPRKLKLSWNAGAVCLAPLPVAIDHGFFLKQNLDVE
                     LVNYSGSTDQLLEAIATGKTDAGLGMALRWLKPLEQGFDVKIAAGTHGGCMRVLSRTD
                     SGVDKLADLKGKTVAVGDLAGPDKNFFSIQLAKQGIDPNKDVDWRAYPGNLLQLAVEK
                     REVQAFLASDPIAYLWLKDNAYKEVASNLDGDYKDKSCCIVGLRGSLVREEPQVARAI
                     TQALLDAAMFTSQNPDKAATSFQPYAPKTASLQDLQAMVRYHTHHHHPVGAVLKRELK
                     AYADDLKLVSVFKPSTDTAKFAERIYVDVFGV"
     gene            complement(9982..10825)
                     /locus_tag="VN968_27480"
                     /pseudo
     CDS             complement(9982..10825)
                     /locus_tag="VN968_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613265.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
     gene            complement(10859..11596)
                     /locus_tag="VN968_27485"
     CDS             complement(10859..11596)
                     /locus_tag="VN968_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012650747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL33009.1"
                     /translation="MTTLQRTAIVVGVGSEQGLGAAIARRFALEGQRVIVSGRTEAKI
                     ARVVRAIVDSGGKAEAFTADATIESEVVALFDFAGASGHLIDLVVFNAGNNVRHDFRT
                     MPAELFEQTWRVATFGGFLVGREAARRLTPSGQGTIIFTGATASLRGKPPFTAFASAK
                     AGLRSLAQSMAREFGPLGIHVAHVVIDGGIDGEKLNTAAPQLRIERGADGLLNIDAIA
                     EAYWHLHGQHRSAWTHELDLRPFKERF"
     gene            12115..12357
                     /locus_tag="VN968_27490"
     CDS             12115..12357
                     /locus_tag="VN968_27490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33010.1"
                     /translation="MIPFYIVRHLAGEFEELSCFRQLEDESAALYFNIVHGAKVRRRF
                     TTQKSGEQCADYVLVARATGTCGLIEEREIALFDAS"
     gene            complement(12391..13602)
                     /locus_tag="VN968_27495"
     CDS             complement(12391..13602)
                     /locus_tag="VN968_27495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXL33011.1"
                     /translation="MKFGIFYELQLPRPWEAGDELKLYQDALTQLETADRCGYDHAWV
                     VEHHFLEEYSHSPSPESFLAAASQRTKNIRLGHGIFQLTTNHPARVAERVAVLDLLSN
                     GRCEFGMGESASITELTPFGRDMETKKEVFEEAVRAIFPMFREGGSEHHGKYFDIPLR
                     NVVPKPVQKPHPPLWMACSQLPTIELAGQNGFGALGFQFVSADAAHAWVHAYYNAITK
                     RLKKLADYEINPNMALVSFFMCAKTDEEARARADGATFFQFALRYYGQGQNRARPAPG
                     TVNMWDEYNKWKRDNPEAQEAALRGGLIGSPETIARKLRRFRSSHIDQVILLNQAGKN
                     SHEHICDSLELFARKVMPEFRADPEHDAWKAGVMSGAIQLEEIDTEAFKDRYGKLAVN
                     VAPAKAAVAAG"
     gene            13776..14885
                     /locus_tag="VN968_27500"
     CDS             13776..14885
                     /locus_tag="VN968_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HXL33012.1"
                     /translation="MPIFTPHQDSALKAVADWLKAKPGKNGTPPVFRLFGYAGTGKTT
                     LARHIADGVDGEVKFAAFTGKAALVMRNKGCDNASTIHSLIYRAKESGVEQPSFELWD
                     DAPASKAKLIVIDECSMVDAELGRDLMSFECPLLVLGDPAQLPPIQGGGFFTDCVPDA
                     MLTEVHRQAQDDPIVRMSMDVREGRELDIGRYGESEVVSRAELDPDRVMGADQVLVGR
                     NNTRRSYNMRVRQKQNIEDPLPVAGDKLVCLRNNRKKGLFNGGLWRVKSRAASKSKII
                     TMRLSPDEDFGHKVTKVSVRGDCFGGAIETIPWEQRKPYDEFDYGYVLTVHKSQGSQW
                     DDVVLFDESFAFQDSRARWLYTGITRAAKRLSVVV"
     gene            15106..15825
                     /gene="msrA"
                     /locus_tag="VN968_27505"
     CDS             15106..15825
                     /gene="msrA"
                     /locus_tag="VN968_27505"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXL33013.1"
                     /translation="MSRSQFSRLSLCAAAVGALAIAAFAIAPSRAAEDAVIVPAPAVD
                     AQASDGIQTAVIAGGCFWGVQGVFQHTSGVVNAMSGYAGGSKATADYNTVSGGSTGHA
                     ESVEIKYDPKKISYGKILQIFFSVAHDPTQLNRQGPDSGTQYRSAIFTTSDEQKKVAE
                     AYIAQLNAAKVYKKPIVTKVGPLEGFYPAEAYHQDYLTLHPNQPYIAYNDLPKVENLK
                     KIFADNYTEKPTLVSSAKVTN"
     gene            15914..16336
                     /gene="msrB"
                     /locus_tag="VN968_27510"
     CDS             15914..16336
                     /gene="msrB"
                     /locus_tag="VN968_27510"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXL33014.1"
                     /translation="MSLSKTMPGKVDRIEKSEAEWRKELTPMQYAVLREKATERPFSG
                     EYEHEQTPGTYTCAGCGQTLFESDAKFDSGCGWPSFTAPAVESHVDEERDVSHGMLRT
                     EVLCSKCNGHLGHVFEDGPGPTGLRYCINSAALKLQPK"
BASE COUNT         3333 a         5337 c         5190 g         3280 t
ORIGIN      
        1 cgatcaggat caacaggtcg ccctgcttga ccagcgcgcc ctcacgaaaa tgaatttcct
       61 ggatggctcc tgcgacccgg gagcgaactt caacccgttc gactgcctcc agccggccgg
      121 aaaactcgtc ccacggcacg gtctcgcggg gcgtgacgac cgataccgaa accggcagag
      181 cagccggcgc cacggcaacc gtctcggcct gcgcagctcc gatccgaccg ttcaggaagg
      241 tcgctccgcc agccatagcc accaccgcga gcaccagccc cgccccaatt aaccgattgt
      301 tttggattat atttttcatg gagaccccat tccgaaggac gttcggggct gcgaaccccg
      361 acttgaacag gctggatcgg ccgaagacgc ccacccaccc tgttacgctg catcgcacac
      421 attcgtaacg cccggtatag atgggccatc gacatcccct gtcaagtcac ttatctaacg
      481 agcggtagga aactcaggag gacccatcat ggccatggga cgtccgcgtg aattcgaggt
      541 cgataaggcg ctagacgttg cgctccaggt gttctggcgc aaaggctacg aaggcgcgtc
      601 gatggcggat ctgaccgagg ccatggggat caccaaaccc agcctttatg ccgccttcgg
      661 caacaaggaa gagctgtttc gcaaggcgct ggatcggtat gtcgacggac ccggcggtta
      721 cttccaggtt gcgctgggaa tgccgaccgc acgcaccgtc gttgaacacc tgctttacga
      781 agcggccgac gccgtcaccg atccgaacaa tccgcccgga tgtcttgcgg tgcagggcgc
      841 gctgtgctgc ggcgatgccg cgcaatccat caaacaggaa ttgatgctgc ggcgtgccaa
      901 tggtgaggaa gatttgcgca aacgcttcga ccgcgccatt gccgaaggcg atttgccggc
      961 aggatctgac gcggccgatc tcgcgcgcta catctccgcg atcttgcaag gcatggcagt
     1021 ccaggcggcg ggcgggacca accgcgacca gttgcgcaag atcgcggaaa tggctttgcg
     1081 aacatggccg ccgacggcga gcatttcgcc aacatagcca tggctggaag gccatatttt
     1141 caaggaagtc accggctcat ttgccacgct ggccgacaag ggcaagggcc tgttcggcgc
     1201 cgctttgcaa aatttcctcg gatagtcgct tgtcgttgac ggcgcgggcg aactgcagcg
     1261 tccccaccat cagcccgtaa agcgcgaccg cctttcgccg tcgctcgcgt tgcgtgcctg
     1321 agtccaggcg ggctgcaatc agctcgatga tgccaaccag cttgtccgtg aatgcgtcac
     1381 gcgcagcctt gggatgccgc gcaatttcgg cgaccagtgc ggaggtcgga cagcctcgac
     1441 cggggttgtc gcgatggccc aaagacagat aatcgcggat cgtgcccacc aatccggccc
     1501 cgctctcggt ggcagcttgc cacatctcgt tgcgcttgcg cgcattgcaa agaacttccc
     1561 gcaacagatc ctccttggag gcgaaatggg cgtaaaacgc gccattggtc agacccgcat
     1621 cggtcatgat gccggcaagg ccgaccgcgg cgacgccccg ttcccggaat tgccgggaag
     1681 caacgtcgat gatgcgctgg cgggtcgcat ccttgtgtcc cttgtcgtag cgcatccttg
     1741 cttcctcctc tcccattgaa ttacgatcgt aatatgttat tacgatcgta atttcatgaa
     1801 tccgcggcat tcgcaagtgg cgaattccgc aactgatggc cgatgaaaaa acaatgagct
     1861 tgcaaaaacc cgtaccgagc gatggcgtgt tgatcggcag cccatcagag ccgaccgccg
     1921 aggctccagc gccggtcgcg gcagccgagg tcgaagccat cggcagaata tcagtggttg
     1981 aaactcccgc cgtccccgca gcgcccgatc atccaaccaa ggcgatgcag acgcgttccg
     2041 ccattttgac agggcctgtt ctgccgacac tgctcaggct ggcgttgccg acgatgacgg
     2101 tcctgctcgc ccagaccgcg gtgaatgtcg ccgaagccta ctatgtcggc tttctcggaa
     2161 cccaagcgct cgccggcgtc gcgctggtgt ttccggtttt catgttgatg atgatgatgt
     2221 cgaacggcgg attaggcagc ggtgtcgcct cggcggtcgc gcgcgccacg ggggccggtc
     2281 acaagcacga cgcggatgcg cttgttctgc acgccgtggt tcttgccgtg atcgtcggcg
     2341 cactgtttac gatcggcgcg gcctctggcg gtccggcctt gtatcaggcg cttggcggcc
     2401 aggatgaaac gctgaatgcc gcactaaaat attccaacta tctgtttgcg ggcgcgatcc
     2461 cggtgtggat cgtgaacttt ctggcggcgg cgttgcgcgg ctcgggtaac gtcagggttc
     2521 cggcgatggt gacactggcc ggcgcgctgg tgctgatccc gttctcaccg atcctgattt
     2581 tcgggttcgg accgatcccc aggctcggga tcgccggcgc cggaattgcg ttcgcgctct
     2641 attatggcgc ggccatgctg gtgctgctgc gctacatgac cacgggacga tcgggcctaa
     2701 cgctaaaatc tggatctctc gagggccggc tgttcaagga cattctgaga gtcggcctgc
     2761 cgacagcgtt cagcacggtc ctcaccaacc tgacggtgat cgtggtgacg ggcgccgtcg
     2821 gacttttcgg caccaccgcg ctcgccgcct atggcatcgc ctcgcggctc gactacgtca
     2881 tgatcccgat cctgttcggc atctgcactt cggtgctgac catggtcggc gtcaacatgg
     2941 gcgctggcca ggtcgcccgc gccaaacgaa tcgcccggat cagtagcttc gtcggtgccg
     3001 gcatcgccgg atcgatcggc ttgattgtcg caattttccc aatgctttgg ctgcgtctct
     3061 tcagccacga tccggacgtg ctggcggtgg gctcaaccta ccttcgtatc gtcgcgccgg
     3121 cctacggtgc cctaggattt ggattcgtga ttgccttcgc cgcacaaggc gccgggcatg
     3181 tgctttggcc cttcgtcggg gcaacctcgc ggctcgttct cgccgccggc tgcggttgga
     3241 tagccgtcag ctatcttggt ggcggcatgg caacgcttgc ggccatggtg agcacctcgc
     3301 tcgttggcta tgccgcgatc tgcgcaatcg cgatgttttc gggagcggtt tggcgggccc
     3361 agaaaggatg agcgccggag acgcgagccg atctaaaggc cggcgccctt cccgatggca
     3421 tgggagaacg aacgatcgac gatatcggcg gcattaagct gctgttttat caggccggag
     3481 cgaaaataca ggtcgatggt cttttgttcg tcggccacga caccatcatc gatcggcgca
     3541 atgcggatct tcgcccggct cagccagttc agcggcaccg cgggcggaat gttcatcagc
     3601 ttgccccagg tctcggcgta agcactgacg ttggtcagcg accaggcgcg ggcggcggtg
     3661 agccgccgaa ggaaatcttc cagttccggc cgcttgtcgc ggatcgcatt ggacgacgcc
     3721 acctggaatc cgattccagc agtcaaaccc tccgccgtaa tcacgcgccg cgactggaac
     3781 agcacctctt cctggctgac atacggctcc catgtcgacc atgcatcgac ggacccttgt
     3841 gtataggcaa ccttggcgtc cgacggcgcc aggaacacaa tctgcacgtc gggcagagac
     3901 catcccctgg attcgagcgc agcaagaatc agctggtgcc cgatcgagcc ccggccggtc
     3961 gcgattttct ttcctctcag atcctcaaag ctgaggatat gagattctcg gggcaccagg
     4021 actgcaagac cttcctggct ctgccggatg gcgccgatcg ctctcaccgg cacgttggcc
     4081 gccgcggcaa aggtgaatgg agcgtcgccg acaagcccgg tttcgatggc gcccgcgccc
     4141 agcgcttcca gcaagggtgc tgccgcggga aattccttcc attcgacctt gtagggtaca
     4201 tccttgagga cgccggccgc ttccatcacg gcctgcgagt tgcccttttg atcgcccacc
     4261 cgcaaggtgg tttgggcggt tgccgaggcc attgcgccca gcaacagccc cgcagcgaag
     4321 ataatgcgtt tcattccgcc gcaactccct tggcgaggcg acgttcggcg atcagcttgc
     4381 gcgtcagggg aatcagttcg cggccatatt cgatggcgtc gatcaaggga tcgaagccgc
     4441 gaataagaaa atggctgacg cccagatcgt aatagtcgcc aaaaacttcg gcgacctgct
     4501 cgggcgttcc gaccagtgcg gtggtgttgc tgttggcgcc ggtgagtctt gccatttcgg
     4561 tccagaggcg tttgtcgatg cgggtgccct gctcggccaa tgccagtagc cgccgcgcgc
     4621 ccgcggtcgc atgaccgtca gccggtttgc gatagccggt cttgtcctgc agggctgttg
     4681 cccgcgccag aatttcctcg gcttttgccc acgccttttc ctcggtgtcg gcaaggatcg
     4741 gccggaccga cagactaaag cgcggagtgg gccgtccatg cgtcgccgcg gcaacctgaa
     4801 cccgcgaggt gacatcgcgc acctgcgcat aggattcgcc ccacagcgca aaagtgtcgg
     4861 catgctttcc cgcaacctcg atcgcggcat cggaggcacc gccgacgaag atgggaatgc
     4921 cttcgctgcg aaatggcttg acttgcgaaa agccgttttg cacctggtag tatttgccat
     4981 tgtacgaaaa cggtttttcg ctggtccatt ccagccgaac cacatcgaga aattcatcgg
     5041 tgcgggcgta acgctcgtcc ttgtcgtcga tggtgttgcc atcctgcttc agctcggtgg
     5101 cattgccgcc ggtgatgaca tgcagggcca cccggccacc ggaaagctgg tcgacggtcg
     5161 caagctggcg ggcaatcaga gtcggtgcgg taaaacccgg ccgctgcgcg atcagcacct
     5221 tgagccggtc ggtcacgccc aggacatgct ggccgacctg caggcagtcc ggcgaggtgg
     5281 aatgaaatgc cagcagagcg cggtcgaacc cggccgtttc atgcgctttg gcgacggtct
     5341 cgatgtaacg gcgatcgagc acagggccgg tccgaacgat ggtctcggat gaattgttgt
     5401 tggtgataaa tccgatgaat tccactgaca ttggcagctc ccgggctccg gttattcttg
     5461 catgcatttc aaatcccgag cgccagccgc ccggcgttga tccgcgtcgc gtcgtcctga
     5521 ggcgtatgaa cgcgcccgca cagcacatca cgatagtatc tttcgagcgg gttggcacgc
     5581 gtcaaaccgt gattgctggt cagcgacagc gcatcctcga ccaccgcaac cgcattgttc
     5641 gtcaccgcga gcttgacgat gttggattcg gatgcgctaa gcaggacgcc gtcatcgtag
     5701 tccccggcaa agctttcgat caggcgcgcg ttgacggcca gccgcgcctc gatgccaccg
     5761 acgacctcct gcatccgcgg cagcgtcgcc agcgacgccc cgaggttgga aggcacccgg
     5821 cttttcagaa attcgatcag ccagtcgcgc gcggcgcggg cgacgccatc ataaatcgcg
     5881 gcgacgaaaa tcgcatgaac cgtcgcctgg gtgaaatcgg gcgtcagcca ttcatcgggt
     5941 ttgcgaacat cgatttcgtg gtcgagcgga aacaggacgt cgtcgaagac aacatcatgg
     6001 ctgccgctgg cgcgcaaacc gagatggtcc caggtctcga tgatgcgcgt ccccggcaat
     6061 ccagccggaa cgagaaacag gccgacgcgc acggtcggct cgtccgttcg cgcccacacc
     6121 gcgtaccatt tcaggatcgg cgcgccggtc gaatagatct ttcggccaga gagccgccat
     6181 cccgtctcgg tcctccgtgc catcgtcgcc ggcaaaccac cgcgcgatgg cgagcccaga
     6241 tcgggctcga cccgcagggc gttgatcagt gccacgcctt cgacggtttc gcgggccagc
     6301 ttgcgtgcaa ggcgacccgg ccaacgcgcg cttctcgtca tcaccagatg ctgaatataa
     6361 tgcatcgaca gcaccagtgc cgtcgacgga tcggcctttc cgaagatgtt gatgatgcgc
     6421 gctgcatcga gcgcgccggc gccggagcca cccagcgccg tcggcacggt cagcgccagc
     6481 aatcccgcct ctgacaactg attgaaattc tcgaacggaa agctcgcctc gcgatcatgc
     6541 agagccgccg tcgcggcaaa ttcggaagca agcagctttg cacagtcgaa tattgccggc
     6601 gttgcatccg tgaaaacttc cttgcgtatc gagttgcctg gcgcgctcac catgttgcgt
     6661 ccaatccgag cagccccaga atgttccggc gcagtaccgc caatttggga tcgccccggt
     6721 gccgcggata gggacgatcg accgcgatgt cggccttgac gcgcgcgggg cgatcgctga
     6781 acacgatcac gcgattggcc agaaacacgg cctcctcgac atcatgggtc accaatagcg
     6841 tggtgaatcc gctgcgctgc cagagcgagg cgatttccgc ctgcatggtg atgcgcgtca
     6901 gggagtcgag cttgccgagc ggctcgtcga gaatgagtat tttgggatcg ttgaccagcg
     6961 cccgcgccag cgcgacccgc tgtgccatgc cgccggaaag ctggtgcgga taggcattgc
     7021 aaaatgtcga gaggcccacg agatcgatgg ccgcatccac ccggtgtcgc tggtgcttga
     7081 ggatgccctg ggcttcgagc ccgagcgcga cattgtccca cacctttcgc catggaaata
     7141 aagtcgggtc ctgaaacacc acgacccgcg acggaaacgg ccccgtgatt ggctcgccat
     7201 cctcgcgcaa gccgcccgat cgcggcggct caagccccgc gaccaggcgc agcaacgtcg
     7261 attttccgca tccggacggg ccgagcaatg cgacgaactc gccgggtttc acgctgaagc
     7321 tgatgtcatc cagtacggga agaacagccc catcgatatc gaacgcatgg ctgacggctt
     7381 cgacatccag cgatgctcca gccacggcgg gtgcggcccg ttccgcgggc gcaagcgcta
     7441 ccatttcacg acacctttct gccagaccag cagccggtcg cgcgtccgaa acagcagcgt
     7501 gatcgcgccc gagcacaata gcgacatcac gatcagcgct gcatacatat tcgaataggc
     7561 cgcccagccc tgggcccact gcagatacca gccaagtccg gccttgacgc cgatcatttc
     7621 ggcgaccacc agcacggcga aggacgaccc gaggcccatg aacaggccga cgaagacgtg
     7681 cggcagcgcc gcggggatag cgaccttcag caccaggaac gatggcttgg cgccaagggt
     7741 tcgcgccacg tcgtaatagg cattgctgac gctggccacg ccggaccagg tcaacaccgt
     7801 gaccggaaat ccggtcgcga gtgcaatcag aaacgtgctc gcgctccagc tcgacggaaa
     7861 ggtaaagaac gcgaacggaa gccatgccgt cgccggcaac ggcccgatga atcgcagaac
     7921 cgggtgtacc cagtagccga cgctgcgcga ccatccaatc gacacgccgg tcaaaaagcc
     7981 ggccgccgca ccgatggcgt agccgccgag ctgaagtttg accgacgcga aaacactatc
     8041 cagcagtttt ggcagatcgt cggtatatac ttcgatgatc gcctgcggcg gcggaaagaa
     8101 cggcagcggc agccaggcga attttgcggt cgcgatctcc cacagggtca aaaacaatcc
     8161 gaacgccagc agccacggcg cacgcgcttg caggcctgtt ccgaaacggc cgagccattt
     8221 accgctcgcc gtcgccgcaa ggataccgcc ggcgatgata aacgcggcga tcgcaagtgc
     8281 atcggtgcgc gaccaatcgc ccaaatcctt ccacagcagg cacgagagcc cgaagccaat
     8341 ccacgccgcg gttgcgaata aggcggttcc actggccaga aaccacgtgg ggagcgacag
     8401 cggacggcct gcaaacgccg cgccctcctc cgccgattgc gggaacgcga ccgtcttaga
     8461 cgccgaatac atcgacatag atccgctccg caaatttcgc tgtgtcggtg ctcggcttga
     8521 agacggagac gagcttcaga tcgtccgcat aggcctttag ctcccgcttg agcacggctc
     8581 caaccgggtg gtgatgatgg gtgtgatagc gaaccatcgc ctgcagatcc tgcaagctcg
     8641 cggtcttggg cgcgtagggt tggaacgaag tcgcggcctt gtccgggttc tgcgaggtga
     8701 acatcgccgc atcgagcagc gcctgtgtga tcgcccgcgc aacctgcggc tcctcgcgca
     8761 caagactgcc gcgcaatccg acgatgcagc agctcttgtc cttgtagtcg ccgtcgagat
     8821 tggaggcgac ttccttgtag gcgttgtcct tcagccagag atacgcgatc ggatccgagg
     8881 cgaggaacgc ctggacttcc ctcttctcga cggcgagttg cagcagattg cctggatagg
     8941 cccgccagtc gacgtccttg ttcggatcga tgccttgctt cgcgagctga atcgagaaga
     9001 aattcttgtc cggtccggcg agatcgccga ccgcgacggt cttgcctttc aggtcggcaa
     9061 gcttgtcgac gcccgagtcg gtgcgcgaca gaacgcgcat gcagccgcca tgggtgccgg
     9121 cggcgatctt gacgtcaaaa ccctgctcca gcggtttcag ccagcgcaac gccatgccga
     9181 gacctgcatc ggttttgccg gttgcaatcg cttccagcaa ttggtcggtc gaaccgctat
     9241 agttgaccaa ctcgacgtcg agattctgct tcaggaagaa accatgatcg atggcgacgg
     9301 ggagcggcgc gaggcagacc gcgccggcat tccatgacaa tttcagcttt cgtggcgcgc
     9361 ccgcgagcgg tacggcctcg gatgacgcct tgcagatcgg aaattccgag aaatcgatat
     9421 tcggagttgc cgcgcggaac ggaaacgcct gcgcgccgaa tacgcctaac ggcgccgcaa
     9481 acgccgtcgc ggcgccggcg cgcaacaatg tgcggcgatc cagcaacgcg ttgccccact
     9541 tcatatctct cgacatcttc ttctcctgct gtcgggcggc acagggccgc cgtcggcgca
     9601 acgcgacgtt gcgacccggc aaatgtttca cactgtcatc agtgaaatcg gttcactctc
     9661 ggcgagattt caactcccgc tccgttgaat tgacgttcaa atgatgcgcg gcagcatcgc
     9721 aatcgtcaat gaaatggaat tttgaatgtg aaagcgaacg gagagattat ctccataagt
     9781 acagcgttgc gccacgaaac ggtttttgct caagcctcaa taacgcgtcg ttcaaagatc
     9841 gtgcgaagct ggctgttcga agatctcttg atacaaagcc gataacgttc cccgacgatt
     9901 caacaaacga aacgatcgtt gctcgcgagc catgccaaag gcaaagggcc ggcatccgat
     9961 gccggccctc tctcgtgagc tttaactccc gtgaaacacc agggtgcgca gttcaatgct
    10021 ctcgcgcggc ggcgcatcgg gaggcgtggt cggatcgatg aacgcgctat ggggcgcgaa
    10081 gcgggccgcg tccgtcggtc ctggaatcaa agcatttcag cagcaagact tcatcggccg
    10141 tcatcctcgg cacatagaac cattgatgat cgggattgta cttcaccgaa taggtttccc
    10201 cgacgcggtt gggatagacc agatcggcag caaccagttc gttgggcttt accgtgcggg
    10261 catcgcagac cgccaggggt gagtcgagca acggacctcg aatcggccgc cagagattga
    10321 tgatctgcac cctgcccttc agcagttcct cagcctcgtc cggaatgaga tcgcggacgc
    10381 gctgcgggcc tgatttttcg gtgtgatcga catgcacgcg cgcaaccggt tgacgcaggc
    10441 cgccatcgcg gtcggcggtg ccttgaacac gcttgcgcac ggtgtgatcg aagacgaata
    10501 cccggtccgc gccggtcgcg gccttgatca gccgttccgc ctccggataa tagacgcttc
    10561 tgacctcgtc ttcgtcgtag aagtccttta ccgaactgtt ttgccgaacc agggcaaacc
    10621 cttctcgatc aagcaaaacc gtctcgctga tcgggcgaat gtcgtgaata ggcaggctgt
    10681 gcggctcggg cacgatattg gacctgggct cgccggacgg cggctcaaag gtataggttc
    10741 gcggcctgtc cagagcaggc gccagatagt tgagatcggc cgtcacgaac gacaacgccg
    10801 cgatcttttg gttttgcagt cccatgttct tctccttttt tgttggctgg tccggcttct
    10861 agaaacgctc cttgaatggc cgcagatcca gttcgtgcgt ccaggcagac cggtgctgcc
    10921 cgtgaagatg ccaataggcc tcagcgatcg catcaatgtt cagcagtcca tcggcgccac
    10981 gttcgatccg aagttggggt gcagccgtgt tgagcttttc gccatcgatg ccgccgtcga
    11041 tgacgacatg cgcgacgtga attcccagcg gaccgaattc gcgcgccatc gattgcgcca
    11101 gcgagcgaag gccggccttg gcggatgcaa atgccgtgaa cggcggcttg ccgcgcaagc
    11161 tggctgtggc gccggtaaag atgatcgtgc cttgacctga cggcgtcaac cgtcgggcgg
    11221 cttcccgtcc caccagaaat ccgccaaagg tcgcgacacg ccaggtctgc tcgaagagtt
    11281 cggccggcat ggtgcggaag tcatggcgaa cgttgttgcc cgcattgaac accacgaggt
    11341 caatgaggtg gccggaagcc ccggcgaaat caaacagcgc caccacctcc gactcgattg
    11401 tggcgtcggc ggtgaacgcc tcggccttgc cgccactgtc gacaatggcg cgaacgaccc
    11461 gcgcaatttt tgcttcggtg cgcccggaaa cgatgacgcg ctgaccttca agcgcgaacc
    11521 gccgcgcgat cgcggcgcca agtccttgtt cggaacctac gcccacaaca atagccgtgc
    11581 gttgaagcgt ggtcattgca ggtatctctc cggtccgatt caatctcgga aaaactatga
    11641 ggctaaatct gtatcgacca ggtggtcccg ccaatggccc gcgaaaaata agattgctgt
    11701 cgcgcaattc gcgcgcgccg tgaaaaattc ttccaaggag aaggacaggc ggagtgaagt
    11761 ccttgttgag taaggacgcc ggccacctcg aaccagaatg cgaacgtctt cggatcagcg
    11821 caggccgctc aagtattgtc tttcgttggt cgccttcgga gaatgttgtg cggacaattc
    11881 acgcaacttt ggaattcaaa atagtgccgg cgtgaccata attatgcgtc ctcgcttttg
    11941 gagttggcgc gtatcacggc catgtgaact tcatcgtcag atcgcagatg attcaacgaa
    12001 gcacgagcta tccaatcaaa atggcagcgc tggtcatcgg ccagatatcg gtcttggccg
    12061 tgctggtctg gatgtctcgc tagaaacaac gcaacgtacc gggcgcgacc aaacatgatt
    12121 ccgttttata tcgtgcgtca tctcgcggga gagttcgagg aactctcctg ctttcggcaa
    12181 ttagaggacg aaagcgcagc gttgtatttc aatatcgtcc acggcgcgaa agtccgcagg
    12241 cggttcacca cccaaaagtc gggcgagcaa tgcgcggact atgtgctggt cgcgagagcg
    12301 accggaacct gtggattgat cgaagaaaga gaaatagcgc tgttcgatgc ctcgtgatcg
    12361 ggcaatctct cattgccccg gagaaaaccg ttatcccgcc gccacggccg ccttggcagg
    12421 cgcgacgttc accgccaatt tgccatagcg atccttgaag gcttcggtat cgatttcctc
    12481 gagctggatg gcgccgctca tcacgccggc tttccatgca tcgtgctcgg gatcggcgcg
    12541 gaattccggc atcaccttcc tggcgaacaa ttcgagcgag tcgcagatgt gctcgtggct
    12601 gttcttgccg gcctggttga gcaggatcac ctggtcgata tgggagctgc ggaaccggcg
    12661 caacttcctg gcgatggttt cgggcgatcc gatcaggccg ccgcgcagcg ccgcctcctg
    12721 cgcttcggga ttgtcgcgct tccacttgtt gtattcgtcc cacatgttga ccgtgcccgg
    12781 cgcgggacgc gcccggttct gtccctggcc gtaatagcgc agcgcgaact ggaagaacgt
    12841 cgcgccatcg gcgcgcgccc gtgcctcctc gtcggtcttg gcgcacatga agaacgacac
    12901 cagcgccatg tttggattga tctcgtaatc cgcgagcttc ttcagccgct ttgtaatcgc
    12961 attgtaatag gcgtgcaccc aggcatgcgc ggcgtcggcg ctgacgaact ggaagccgag
    13021 tgcaccgaaa ccgttctggc ccgcaagctc gatggtcgga agctgcgaac acgccatcca
    13081 caatggcgga tgcggcttct gaacgggttt gggcacgacg ttgcgcaggg gaatatcgaa
    13141 atatttgccg tgatgctcgc tgccgccctc cctgaacatc ggaaagatgg cgcggaccgc
    13201 ctcctcgaac acttccttct tggtttccat gtcgcggccg aatggcgtca gctcggtgat
    13261 cgaggcgctt tcgcccattc cgaactcgca gcgtccgttg ctgagcagat cgagcaccgc
    13321 gacgcgctcg gcgacgcgcg ccgggtgatt ggtggtgagc tggaaaatgc catggccgag
    13381 ccggatgttt ttggtgcgct ggctggcggc ggcgagaaac gattccggcg acggtgaatg
    13441 cgaatactcc tcgagaaaat ggtgttcgac cacccaggca tggtcgtagc cgcagcggtc
    13501 cgcggtctcc agttgcgtca acgcatcctg gtagagcttg agttcatcgc cggcttccca
    13561 cgggcgcggc aattgcagct cataaaagat gccgaatttc atgacgtctc tctcccgcga
    13621 tccgtttgtt gttttgcgca gtgtattccc cggcggggcc gtgtccagcc gtcgtcaatt
    13681 ccgcgcggcg gaaggcgact tgcgctaaca tcacgaatgg ttagcttgcg cgctgagtcg
    13741 tcgcaccgct ccgccgtgtc ctgcatcgag atttcatgcc cattttcacg ccgcatcagg
    13801 attccgcgct aaaggccgtt gccgactggc tgaaagcaaa gcccggcaaa aacggcacgc
    13861 cgccggtgtt tcggctgttc ggctatgccg gaaccggcaa gaccacgctg gcgcggcaca
    13921 tcgccgacgg cgtcgacggc gaggtcaagt tcgcggcctt caccggcaag gccgccctgg
    13981 tgatgcgcaa caagggctgc gacaacgctt ccacgatcca ttcgctgatt taccgcgcca
    14041 aggaatccgg cgtggagcag ccgagttttg agctgtggga cgacgcgccg gcctcgaagg
    14101 ccaaactgat cgtgatcgac gagtgttcga tggtggacgc cgaactcggc cgcgacctga
    14161 tgtcgttcga atgtccgctg ctggtgctcg gcgaccccgc gcaactgccg ccgatccagg
    14221 gcggcggctt ctttacggac tgcgtgcccg acgcgatgct gaccgaggtg catcgccagg
    14281 cgcaggacga tccgatcgtg cggatgtcga tggacgtgcg cgagggccgc gaactcgaca
    14341 ttggccgcta cggcgaaagc gaggtggtgt cgcgcgccga gctcgatccg gaccgggtga
    14401 tgggcgccga ccaggtgctg gtcggccgca acaacacgcg gcgctcctac aacatgcggg
    14461 tgcggcagaa gcagaacatc gaggatccgt tgccggtcgc cggcgacaag ctggtctgcc
    14521 tgcgcaacaa ccgcaagaaa ggcctgttca acggcgggct gtggcgggtg aaatcgcgcg
    14581 cggcgtcgaa atcgaagatc atcaccatgc ggctgtcgcc cgacgaggat ttcggccaca
    14641 aggtgacaaa ggtctcggtg cgcggcgact gtttcggcgg cgccatcgag accatcccgt
    14701 gggagcagcg caagccctat gacgagttcg actatggcta tgtgctcacg gtgcataaat
    14761 cgcagggctc gcagtgggac gacgtggtgc tgttcgacga gagctttgcg ttccaggaca
    14821 gccgcgcacg gtggctctac accggcatta cgcgcgccgc gaagcggctg agcgtggtgg
    14881 tgtagcctcg cctcagcaac tcgtccccgc cccgcgcatc ggggttccca cgaccctaac
    14941 atcctggaaa tgcgctgttt ttattcacga actcgtgtgc gtcacgctgt aacaatgccc
    15001 gcctggccgc gtatcttggg gcatcgggca gagccgcctg cgattgcgaa cggtggcttc
    15061 cgcctaaaac tgctgccaaa tccggccacc ccagaggaag cccccatgtc ccgtagccaa
    15121 ttcagccgcc tttcgctctg cgccgccgcg gtcggcgcgc tggcgatcgc cgctttcgcg
    15181 atagccccct cgcgcgcggc ggaagacgcc gtgattgtcc ccgcgcccgc cgtggacgcc
    15241 caggcgtccg atggcatcca gaccgccgtg atcgccggcg gatgtttctg gggggtgcag
    15301 ggcgtgttcc agcatacgtc gggcgtggtc aacgccatgt ccggttatgc cggcggcagc
    15361 aaggcgaccg ccgactacaa cacggtctcg ggcggatcga ccggccatgc cgagtcggtc
    15421 gagatcaaat acgacccgaa gaagatcagc tatggcaaga tcctgcagat cttcttctcg
    15481 gtggcgcacg atccgaccca gttgaaccgc cagggtccgg actccggcac gcaatatcgc
    15541 tcggcgatct tcaccaccag cgacgagcag aagaaagtcg ccgaggccta tatcgcgcag
    15601 ctcaacgccg caaaggtcta caagaagccg atcgtgacca aggtcgggcc gctggaaggg
    15661 ttttatccgg ccgaagccta tcaccaggac tacctgacgc tgcacccgaa ccagccctat
    15721 attgcctata atgatcttcc gaaggtcgag aacctgaaga agatctttgc ggataactac
    15781 accgagaagc cgacgctggt gagcagcgca aaggtcacca actgacggcg atttcaacga
    15841 gttgaccgtg tttccttcac gcggcccggt atcggcttcg cgtgaaaaca cgcaacattt
    15901 gcaaggaggt ttcatgtccc tttccaagac gatgcccggc aaggtcgaca ggatcgaaaa
    15961 gagcgaggcc gagtggcgca aggaattgac gccgatgcag tacgcggtgc tgcgcgagaa
    16021 ggcgaccgag cggccgttct ccggcgaata cgagcacgag caaacgccgg gcacctacac
    16081 ctgtgccggc tgcggccaga ccctgttcga atccgacgcc aaattcgatt ccggctgcgg
    16141 ctggccgagc tttaccgcac ccgcggtcga gagccatgtc gacgaggagc gcgacgtcag
    16201 ccacggcatg ctccggaccg aggtgctgtg ctcgaagtgc aacggccatc tcggccacgt
    16261 gttcgaggac ggccccggcc cgaccggact gcgctactgc atcaattcgg cggcgctgaa
    16321 gctgcagccg aaataaactt ggccatcaga tcgtcatcac cgggcttgaa ccggtgatcc
    16381 atcttcttcg aaaaaagttg tcacgaagat tgatggatgc ccggatcaag tccgggcatg
    16441 acgagggtgt ttcgccggtc gaggtcaagg cgcggttgcg cactattttt cagatttaat
    16501 tttcaaacaa ccacgacaat cagtttgcag caccgtcatt gcgagccatc gggcgcgcat
    16561 tcgcgcgacc cgttggctcg caatgacggt gctctaattc cagacacgcg ttcgcgaccc
    16621 cgcggcgcgg cgcgcccgag tcgtgcattt atcgctcgcc ccatggaggg cgtggggaat
    16681 gccggatgcc cgttgcaccc gcggcctcgt gtgcactttg tattggtaga aagcacacga
    16741 gtaacaacga gtacaccgga atcacccggc attcccgcac gcaatggttt taacagctta
    16801 tgtcgcgctc tccccggtga ccgggctctc ttgccaccgt catccgcgga tatctgactt
    16861 gtccgcaccc ggtagggccg acttgccatc cgcaaaactt gcgtcggggc gtcaggacca
    16921 cacgattttg ccgtccgcag caacatctct cgtcagcgtg ctgttgatcg ctcacgaatt
    16981 cctgagaacc cgccctgcga tcccatcgcg cgcaaaacgc tgccgcgtcc accgcatccc
    17041 tccccgcgtc cgtgacgatc gcgatacgcc cctctggtgg ggtgggatgg cgcgaacttg
    17101 tagagatgat ttgcccgacg ggtgaagcga aatatttttg
//
LOCUS       DATNOI010000687         7552 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_584234,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000687 DATNOI010000000
VERSION     DATNOI010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584234"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..992)
                     /gene="hpaD"
                     /locus_tag="VN968_27515"
     CDS             complement(9..992)
                     /gene="hpaD"
                     /locus_tag="VN968_27515"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HXL33015.1"
                     /translation="MPVPQHIFDPPFNIIRCSHVVLDVVDLNASREFYENNVGLHVED
                     RDDEAVYLRGSEEHQHHSLVLRKVGTAGCNRLGFKVGNEQDLDKAAAFFSENGISYAF
                     AEQPFQGRTLQFADPFGFQLELYASMDKRPHLLRRYDLYKGCHPQRLDHFNVFAAEVQ
                     DTVDFYARIGFRLTEYAEEDGPRGRIAAAWMHRKGNVHDFAITNGKGPRLHHFAYWVP
                     SAMNILHLCDVMASTGYLKNIERGPGRHGISNAFFLYIRDPDGHRLELYTSDYFTMDH
                     DHEPMRWSLKDPRRQTLWGAPAPRSWFEQGSPFPGQAVREPLFVADVTIGD"
     gene            complement(1016..1423)
                     /locus_tag="VN968_27520"
     CDS             complement(1016..1423)
                     /locus_tag="VN968_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HXL33016.1"
                     /translation="MPHFTIEYSANLDARLDMGAVVEIIRKAAVETGIFPLGGIRVRA
                     IRCEHYAIADGRSDLGFLDMVLRLGEGRDVATRKKAGEHIFRVLSDHLDLVFAQGPFA
                     LSFDMQINDKQTSWKRNNIHEQFTTEAKRRVPE"
     gene            1790..2935
                     /locus_tag="VN968_27525"
     CDS             1790..2935
                     /locus_tag="VN968_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL33017.1"
                     /translation="MKLTRRDFTAGLAVGITAPYVIGTACAQGATIKIGMCVPVTGPA
                     AEQGLWAQNGAKLALAAVNKAGGVLGKQVELVIEDDQTTNPGIVLAFSKLAAQPDIVG
                     FLGSIRSTQVHAMAPDVLKLGKPVMIGGTDPTLTHMGNQWLFRFRPNDSYSGRVIADY
                     GINTLGKKKWAIVHSTDAFGTAGGKALASALEKLGAPAVLDQGYANQSQDFTPVVLAV
                     KQSGADVLGTYFTFENDLGIFARQLRQLGVNIPWVGSPSVVAVSSTKLAGPALYGTYG
                     VADYAEESSEASKTFGKAYREAYKTAPDNQSAWPYDAVTTLSAAINKAGSTDPGKIRE
                     ALLATKKTPGAEGEYNFDQNGDGLHGYNVVRNEKGTIVYDKHIDFND"
     gene            3078..3953
                     /locus_tag="VN968_27530"
     CDS             3078..3953
                     /locus_tag="VN968_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HXL33018.1"
                     /translation="MDLALQLLFTGIGIGAVYALVALGFVLIFRATNVVNFAQGEFSM
                     VAAYLMVVFAVDLGWPYWLSFLLALAGMALLGAIFNLGVYYPLRHRTFLPVIIATIGA
                     SILLANSVLAIYGPQPQVLEGWFETPGIQLGPVYLDSQYLLIIAVTIVLVIFNYWFFE
                     HTVLGKKLQATSQDKEMASLLGISVSSMIMITFIYSAVLGGLAGILVAPILFVSIQMG
                     STIALKAFAATIIGGFGDVTGAIIGGLSLGIIETFGAAYISVPYKDGFAFLVLVLFLI
                     FRPQGIFGERVAEKA"
     gene            3950..5731
                     /locus_tag="VN968_27535"
     CDS             3950..5731
                     /locus_tag="VN968_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HXL33019.1"
                     /translation="MSAPSENMPIAAVVPRARPLLLRHLPYFIGAAILVTLAATMRFD
                     GYIHNILMQATTFAIAVFGLSVVLGLCGQINLAQAAFFGLGAYAVGLGTSDYHLSYWL
                     CLAGGCVIALLAGALLGMSTLRLGGHYLAMVTISFQQIVTLVMINTIWLTHGPDGVSR
                     IGRPDLFQSAQSYLAFCIAMLALVGYFVWHLSDTRLGRAMRAVRDNELAAGVVGIDVF
                     RTKVSAFALSAVLGGLAGGLFAGGFAYVSPDQFSFAESVVFLTMSLLGGVASPIGSAI
                     GTGLLILIPEWLRFLKSVPGLYLAIYGLSVILIIRYMPDGIWGFVNIATERWRAQKGA
                     PPAAGPLQLMPATVGGDTVLEVSGLSKYFGGLKAVDGVDISVKRGGVHALIGPNGSGK
                     TTTLNVLSGLYKATAGRILLDGTDITSMPPHQRTAAGLGRTFQNIRLFRSMTALENVE
                     IGAERPGNRMIGKGGDEALTERAMEALTFVGLGGRANEMISSFSYGHQRLIEIARALA
                     ANPTLLLLDEPAAGLNSSEKLELHELLKRIAAQGLTILIIDHDMTLVSEAAQHITVLN
                     FGRRIADGESMAVLRHPDVVSAYLGTE"
     gene            5731..6459
                     /locus_tag="VN968_27540"
     CDS             5731..6459
                     /locus_tag="VN968_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL33020.1"
                     /translation="MPLLEINDLIVLYGEIEALRGVSISVDEGHVVTLLGANGAGKST
                     TLRAISGLAKPAAGEILFEGKSIAGLGPEAIVRLGISHVPEGRRIFPGLSVKENIMLG
                     ASNRRVAKSQLSREADAMFDLFPDIRSFSNALGWTLSGGQLQMVAVARGLMAKPRLLL
                     LDEPSLGLAPVIVQAVFRIISEIRRNTTVLLVEQNARMGLSVADHGYVLETGRIVLGG
                     KPDELWGNEAIRAAYLGGHAKVSA"
     gene            6477..>7552
                     /locus_tag="VN968_27545"
     CDS             6477..>7552
                     /locus_tag="VN968_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HXL33021.1"
                     /translation="MVTVQVQRLIDFVAEVFSRSDSSPEEARRIATYLTTANLTGHDS
                     HGVIRVPVYIRWKKMGSVVPDQTVERVVDTPSLAVVDGRFGYGQTVAPQAVGIGIEKC
                     KASGLAAVALRNAGHIGRVGDWAEMAAAEGLVSVHFVNAAGSVLVAPCGGVERRMSTA
                     PYCVGIPRQGQGPIVLDFATSIVAEGKVLVASRGGKKLPKGALIDLDGNLSEDPAVLY
                     GPHTAEGPRDHGQGKGAIRAFGEHKGSGLAFICELLGGALTGTGATGPNRRFANGMFA
                     VYVDPKVIDTSNFFDGEISRYVTYFKDTRPAAGIDAVLIPGEPEAKMRAERTKNGVPL
                     PDDTWAAIVNTAREVGVSEASIQRA"
BASE COUNT         1383 a         2303 c         2373 g         1493 t
ORIGIN      
        1 gatcaactct aatcgccaat ggtgacatcg gcgacaaata gcggctcgcg caccgcctgt
       61 cccgggaacg gcgatccctg ctcgaaccag gaacgcggcg ccggcgcgcc ccacaacgtc
      121 tgccgccgcg ggtccttgag cgaccagcgc atcggctcat gatcatggtc catggtgaag
      181 taatcgctgg tgtagagttc cagccgatga ccatcggggt cgcggatata aagaaagaac
      241 gcatttgaaa tgccgtgccg gcccggcccg cgctcgatat ttttgagata accggtggag
      301 gccatcacgt cgcacagatg caagatgttc atggcactcg gcacccagta cgcaaaatga
      361 tgcagtcgag ggcccttgcc gttggtgatg gcgaagtcat gcacgttgcc cttgcggtgc
      421 atccacgccg cggcgatccg accccgcggc ccgtcttcct cggcatattc ggtcagccga
      481 aaaccgatcc gcgcgtagaa gtcgacggtg tcctggacct cggcggcgaa cacgttgaag
      541 tggtcgagcc gctgcgggtg acaacccttg tagagatcgt agcggcgcaa cagatgcggg
      601 cgcttgtcca tcgacgcgta gagttcgagc tgaaagccga agggatcggc gaactgcagg
      661 gtgcggccct gaaacggttg ctccgcaaac gcgtaactga tgccgttttc cgaaaagaac
      721 gccgcggcct tgtcgaggtc ctgctcgttg ccgaccttga agcccaggcg gttgcagccg
      781 gcggtgccaa ccttgcgcag caccagcgag tggtgctgat gctcctcgct gccgcgcaag
      841 tacaccgcct cgtcgtcgcg atcctcgaca tgcagcccga cgttgttttc atagaactcg
      901 cggctggcat tcagatcgac cacgtcgagc acgacgtggc tgcacctgat gatgttgaaa
      961 ggcggatcga agatatgttg cgggacgggc atgcggtgtc ctcaactcga tttcgtcatt
     1021 ccgggacgcg ccttttggcc tcggtggtga actgttcgtg gatgttgttg cgcttccagc
     1081 tggtttgctt gtcgttgatc tgcatgtcga acgacagcgc gaacgggcct tgcgcaaaca
     1141 cgagatcgag atgatcggac agcacgcgga aaatatgctc gccggccttc ttgcgggtcg
     1201 caacatcgcg gccttcgcca agacgcagca ccatatcgag gaaaccgaga tcggaccgtc
     1261 cgtcggcgat ggcgtagtgc tcgcagcgga tcgcgcgaac ccgaatacct ccgagcggaa
     1321 agatcccggt ttccaccgcg gccttgcgga tgatctcgac gaccgcgccc atatcgaggc
     1381 gcgcatcgag gttggctgaa tattcaatcg tgaaatgcgg catgtccgga tcattccaaa
     1441 gtattcttca gggcacttca agcgaagtaa acagctcacc gtatcatgcg gatcacaatc
     1501 ggcttgaatc gtcttgccgg cgagcctgtg cgagttgtga ggccagcgag cttcgcaacc
     1561 agtaaggcaa atttgcatga tgccgtgaac gcactgcata gctcaagggc cacttgtcgt
     1621 ttgagcaggc aaaccacggc tcaatttggc cgagcgaggt gcccgagtgg agatttgatg
     1681 cgatatcaat ttactcacgc tgcgacacgc cgtagacacg acgcacggtt tttcgctcaa
     1741 acccaaactt gaagaaggcc agcgctgaag ctggccgcgg gaggactgaa tgaaacttac
     1801 gagacgtgac ttcaccgctg gcttggccgt tggtatcacc gcaccttacg tgatcggcac
     1861 cgcgtgcgcg cagggagcga ccatcaaaat tgggatgtgc gtgccggtga ccggacccgc
     1921 ggccgagcag gggctttggg cccagaatgg cgcgaagctc gcgctcgcag cagtgaacaa
     1981 ggctggcggc gtactcggca agcaagtcga gctggtgatc gaggacgacc agaccaccaa
     2041 tccaggcatc gtgctggcgt tttcgaaact ggccgcgcag ccggacatcg tcggattcct
     2101 gggttcgatc cgctcgaccc aggtgcatgc gatggcgccc gatgttctca agctcggcaa
     2161 gccagtgatg atcggcggca ccgatcccac cctcacccac atgggcaatc aatggctgtt
     2221 ccgcttccgc cccaacgaca gctattcggg gcgggtcatc gccgattatg gcatcaacac
     2281 gctcggcaag aagaaatggg cgatcgttca ctcgaccgac gcatttggca cggcgggcgg
     2341 aaaggccttg gcctcggcgc ttgaaaaact cggcgccccg gcggttctcg atcagggcta
     2401 cgccaatcag agccaggatt tcacgccggt agtgctggca gtgaagcagt ccggcgcgga
     2461 tgttctcggc acctatttca ccttcgaaaa cgatctcggc attttcgctc gccagctgcg
     2521 ccagctcggc gtcaacattc cctgggtggg ctcgccttcg gtggtggcgg tatcatcgac
     2581 caagctggca ggtccggcac tgtatggcac ctatggggtt gcggactacg ccgaagagtc
     2641 cagcgaggct tcgaagacct tcggcaaggc ctatcgcgag gcttacaaga ccgcgccgga
     2701 caaccagagc gcctggccgt acgatgccgt caccaccctg tcggccgcga tcaacaaggc
     2761 cggctcgacc gaccccggga aaatccgcga ggcgctgctc gccaccaaga aaacccccgg
     2821 cgcggagggc gaatacaatt tcgaccagaa cggcgacggt ctgcacggct acaacgtcgt
     2881 gaggaacgag aaggggacga tcgtctacga caagcatatc gacttcaacg attgatcgcg
     2941 tcgccggccc ctccgatgcg gaggggccag caacgttttg atgagcagcg cgcccgtacc
     3001 ccgttgccgc cggtcgtcct tccaagcgaa tactgtagcc ggagacggct cgcagcttct
     3061 cttaaccgag catttccatg gatctcgcgc tacaattgtt gttcaccgga ataggtatcg
     3121 gcgcggtcta cgcgctggtc gcgctgggtt tcgtgctgat cttccgcgcc accaacgtcg
     3181 tgaacttcgc gcagggcgaa ttctcgatgg tcgcggctta tttgatggtg gtgttcgcag
     3241 tcgatctggg atggccgtac tggctctcct tcctgctggc gctggccggc atggcgctat
     3301 tgggcgcgat cttcaatctc ggggtctact atccgctgcg tcaccgcacc ttcctgccgg
     3361 tgatcatcgc gaccatcggc gcctcgatcc tgctcgccaa ctcggtgctc gcaatctacg
     3421 gcccgcagcc gcaggtgctg gaggggtggt tcgagacgcc gggcattcag ctcgggccgg
     3481 tctatctcga cagccagtat ctcctgatca tcgccgtcac gatcgtactg gtgatcttca
     3541 attactggtt cttcgaacac accgtgctgg gcaagaagct gcaggcgacc tcgcaggaca
     3601 aggagatggc gtcgctgctc ggcatctccg tgtcgagcat gatcatgatc accttcatct
     3661 actcggcggt gctcggcggt ctcgccggca ttctggtagc gccgatcctg ttcgtttcga
     3721 tccagatggg ttcgaccatc gcgctcaagg catttgcggc caccatcatc ggcgggtttg
     3781 gcgacgtcac cggcgcgatc atcggcggac tttcgctcgg catcatcgaa accttcgggg
     3841 cggcctacat ttcagtgccc tacaaggatg gctttgcctt cctcgtgctg gtgttgttcc
     3901 tgatcttccg gcctcagggc atcttcggcg aacgcgtcgc ggagaaggca tgagcgcccc
     3961 cagcgagaat atgccgatcg ccgcggtcgt cccgcgtgca aggccgctgc tgctgcgtca
     4021 tctgccgtat ttcatcggcg ccgcgatcct ggtgacgctg gccgccacga tgcggttcga
     4081 cggctacatc cacaacatcc tgatgcaggc gacaaccttt gcgatcgccg tgttcggtct
     4141 ctcggtcgtg ctcggcctct gcggccagat caatctggcg caggctgcgt tttttggttt
     4201 gggcgcctat gccgtcggcc tggggacatc ggactatcat ctcagctatt ggctctgcct
     4261 tgcgggtgga tgcgtcatcg cgcttttggc cggcgcgctc ctgggcatgt cgacgctgcg
     4321 gctcggcggc cattatctgg cgatggtgac gatctcgttt cagcagatcg tcacgctggt
     4381 catgatcaac acgatctggc tcacccacgg cccggacggc gtatcgcgga tcggccggcc
     4441 cgatctgttc cagtccgcgc aatcgtacct ggctttctgc atcgccatgc tggcgctggt
     4501 cggctatttc gtgtggcacc tgtcggatac ccggctcggg cgcgccatgc gcgcggtgcg
     4561 cgataacgag cttgcagccg gcgttgtcgg catcgatgtc ttccgcacca aggtttccgc
     4621 gtttgcgctc agcgcggtgc tgggcggatt ggccggcggt ctgttcgcgg gtggttttgc
     4681 ctatgtcagc ccggatcagt tctcgtttgc cgagtcggtt gtgttcctca cgatgtcctt
     4741 gctcggcggc gtggcctcgc cgatcggatc ggcgatcggc acgggtttat tgatcctgat
     4801 tccggaatgg ctgcggtttc tgaaaagcgt tcccgggctt tacctcgcga tctacggatt
     4861 gtccgtgatc ctcatcatcc gctacatgcc cgacggcatc tggggattcg tcaacatcgc
     4921 aaccgagcgc tggcgcgccc agaagggggc gccgccggcg gccgggccgc ttcaattgat
     4981 gcctgcgacg gtcggcggtg acacggtgct ggaggtcagc gggctgtcga agtatttcgg
     5041 cggcctcaag gcggtcgacg gcgtcgatat ctcggtgaag cgcggcggcg ttcatgcact
     5101 gatcggaccg aacggctccg gcaagaccac aactctgaac gtcctctccg gcctctacaa
     5161 ggcgaccgcc ggcaggatac tgcttgacgg caccgacatc accagcatgc cgccgcatca
     5221 gcgaaccgca gccggattgg gacggacgtt tcagaacatc cggctgttcc gatcgatgac
     5281 ggcgttggaa aacgtggaaa tcggcgccga gcgtcccggc aacaggatga tcggcaaggg
     5341 tggcgacgag gcgctgaccg aacgcgcgat ggaagcgctg acgttcgtcg ggctcggcgg
     5401 gcgagccaac gaaatgatct ccagtttttc ctatggtcat cagcgattga tcgagatcgc
     5461 gcgcgcgctt gccgccaatc cgaccttgct gctgcttgac gaacctgcag ccggcttgaa
     5521 ctcgagcgaa aagctcgagc tccatgagct gctcaagcgc atcgccgccc agggcctgac
     5581 catcctgatc atcgatcacg acatgacgct ggtctccgag gcggcccagc acatcaccgt
     5641 gctcaatttc gggcgccgca tcgcggacgg cgaatccatg gcggtgctgc gccaccccga
     5701 cgtcgtctcc gcctatctgg ggaccgaatg atgccgttgc tcgaaatcaa cgacctgatt
     5761 gtgctctatg gcgagatcga agctttgcga ggcgtgtcga tcagcgtcga tgaaggacac
     5821 gtggtgacct tgctcggcgc caacggtgcc ggcaaatcga ccacgctgcg ggcgatctcg
     5881 gggctggcaa agccggcggc aggcgaaatc ctgttcgagg gcaagtcgat cgccggcctt
     5941 gggccggagg ccattgtgcg tctcggcatc tctcacgttc ccgaagggcg gcggattttc
     6001 ccaggcctgt cggtcaagga aaacatcatg ctcggcgcct cgaaccggcg cgtcgccaaa
     6061 tcccaactgt cgcgcgaagc cgacgccatg ttcgacctgt ttccggacat caggtcgttt
     6121 tccaatgcgc tcggctggac cttgtcgggc ggccaattgc agatggtcgc agtcgcgcgt
     6181 ggcctgatgg cgaagccacg ccttttgctg ctggacgagc cgtcgcttgg cctcgcgccg
     6241 gtcatcgtgc aggcggtgtt ccggatcatt tcggagatcc ggcgcaacac gacggtcttg
     6301 ctggtcgagc aaaatgcccg catggggctt tcggtcgccg accacggcta cgttctggaa
     6361 accggccgca tcgtgctggg aggcaagccc gacgagttat ggggcaacga agccattcgc
     6421 gccgcctatc tcggcggtca tgccaaagta agcgcttaaa agtgggctaa ggattcatgg
     6481 tcactgtaca agttcaaaga ctgatcgact tcgtcgccga agtgttctcg cgttcagact
     6541 cctcgcctga agaggcgagg cgcatcgcga cctatctgac aaccgccaat ctcaccggac
     6601 acgacagcca cggcgtgatc cgggtgccgg tctatatcag gtggaaaaag atgggctcgg
     6661 tggttcccga tcaaaccgtc gaacgcgtgg tcgatacgcc gtcgctcgct gtggtcgatg
     6721 gcaggttcgg gtacgggcaa accgtggcgc cgcaggcggt cgggatcggc atcgaaaaat
     6781 gtaaagcctc gggcctcgct gccgtcgcct tgcgcaatgc cggacatatc ggccgcgtcg
     6841 gcgactgggc ggagatggcg gcagccgaag gactggtatc ggtccacttc gtcaacgccg
     6901 cgggctcggt gctggtggca ccctgtggcg gggtcgagcg gcggatgtcg acggcacctt
     6961 attgcgtcgg cattccacgc caggggcagg ggccgattgt gctcgatttc gctacctcga
     7021 tcgtcgccga aggcaaggtg ctggtcgcaa gccgcggcgg caagaaattg ccgaagggcg
     7081 cgctgatcga tctcgacggc aatctgagcg aggaccctgc ggtgttgtat gggccgcaca
     7141 ccgcggaagg gccgcgcgac cacggccagg gcaagggcgc gatccgcgcc ttcggcgagc
     7201 acaagggctc gggtctggcg ttcatctgcg aattgctcgg cggcgcgctg accggtaccg
     7261 gtgccaccgg gccgaaccgg cgttttgcca acggcatgtt cgcggtctat gtcgatccaa
     7321 aagtgatcga taccagcaat ttcttcgatg gcgagatttc gcgctacgtc acctacttca
     7381 aggataccag gccggccgcc ggcatcgatg ccgtgctgat cccgggcgag ccggaagcaa
     7441 aaatgcgcgc cgagcgcacc aaaaacggtg tgcccttgcc tgacgatacc tgggcggcga
     7501 tagtgaatac cgcccgcgaa gtcggcgtga gcgaggcgag catacagcgg gc
//
LOCUS       DATNOI010000688        10023 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_98261,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000688 DATNOI010000000
VERSION     DATNOI010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10023
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98261"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VN968_27550"
     CDS             complement(<1..611)
                     /locus_tag="VN968_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXL33022.1"
                     /translation="MNDIVLQKLDQGLLTITMNRPDRRNALNPDMTRGLVEAARRAAE
                     DHEVRAVLLKGAGGTFCVGGDVKSMAEGRAPLPFEARMANLRRGMEVSRILHDMPKPM
                     VAQVDGAAAGAGLSIALACDLRVASASVKITTAFAKVGLSGDYGGTYFLTHLLGSAKA
                     RELYLMSPVLSAQEAFALGIVTKVVPDAEVEAAAHELAMSLAQG"
     gene            complement(735..1940)
                     /locus_tag="VN968_27555"
     CDS             complement(735..1940)
                     /locus_tag="VN968_27555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL33023.1"
                     /translation="MNRVLSGVLASALVLAATAAMAQSKPPLKLGGILDMSGLYADIT
                     GPGSAIAAKMAAEDFGGEVLGRKIEIIAADHLNKADLAANIARDMLDNQGVEMIFDVA
                     ASATALAAGEIAKTRNKIIIFNGPGSIRLSNEACGPYTVHYVFDTFAQANVTGLAAVK
                     QGLDSWFFLTADYAFGQDLEKDTTNVVLKSGGKVLGSVRHPLNTSDFSSYLLQAQSSK
                     AKVIGLANAGGDTINAIKQAAEFGLTRSGQKLSPLLAFVTDIDSVGLETAQGLLLAEA
                     FYWDLNDDTRAFSKRFMERVKRVPTAAQVGVYSSVTHYLKAVKAAGTTDSAAVMKIMK
                     DTPINDVFAKQGRIRQDGRMVHDMYLFEVKKPSESKGRWDDYKLLATIPGNEAFQSLE
                     LSRCPLVKK"
     gene            complement(2133..2546)
                     /locus_tag="VN968_27560"
     CDS             complement(2133..2546)
                     /locus_tag="VN968_27560"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXL33024.1"
                     /translation="MEVNGIAHIFLTASNFDRSREFYRKLLPFLGLKPVIDTDAVYYC
                     VGGRTAVAIRAPAAEHAGAAFEQNRVGLHHLCFRARERADVDELHGFLCSHGVTIVRA
                     PREDQWAPGYYSLLFEDPDGIRLELNHVPGKGLLS"
     gene            2709..3449
                     /locus_tag="VN968_27565"
     CDS             2709..3449
                     /locus_tag="VN968_27565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HXL33025.1"
                     /translation="MLVAAAAGPADAAGCAFEPQGEGRVAAVIDARSFRLQDGREVRL
                     AGIEPVAVEKDHASRISALAAIVMGRDVTLRGEDDSPDRYGRQPAFVFLDGTETSVQG
                     LLLTQGEALVSATVADKDCALSLLAAETAARDAKRGTWADPAAIKNAESPGDILTGIG
                     RFTVVEGKVLSVRQAGATTYLNFGRSWTQGFAVTISRRATAALEIAGIIVKSLENRRI
                     RVRGWVEARSGPRIEVLRAGQIELLGEN"
     gene            3510..4961
                     /locus_tag="VN968_27570"
     CDS             3510..4961
                     /locus_tag="VN968_27570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXL33026.1"
                     /translation="MSRRLWAAPMLLCAAFALSACSDVAKFQTPIPTASPLKARTVAQ
                     TPAAEREHERILSSYGGSYDDPNLEALIGKTVDRLVAASDRPDQAYKVTILNSGAVNA
                     FALPTGQLYVTRGLVALASDTSELSSVLSHEMAHVLAKHAAIREDQARQAAVVTRVVT
                     DMGNDPDLTALALAKTKLTMASFSRAQEFEADAIGVSISARAHFDPYGAARFLTSMER
                     NAEMKAGKTSLDPRAQDFLSSHPATPERVENAQATARQYTSPASGERDRETYLAAIDN
                     IVYGEDPSEGFVRGRRFLHPKLGFTFAAPENFTLDNTAQAVIGVREGGTQAMRFDVVR
                     VPAEQSLGDYLNSGWMENVDKPSTEDLTINGFPAASAQARGEQWQFRVYALRFGSDVY
                     RFIFATKEKTTESDRNARETVNSFRRLSLEEIQAARPLRIKIITAQPGDTVESLSHRM
                     AAVDRPMERFRVLNGLDAHAQVKVRDLVKIVVD"
     gene            complement(5176..5829)
                     /locus_tag="VN968_27575"
     CDS             complement(5176..5829)
                     /locus_tag="VN968_27575"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HXL33027.1"
                     /translation="MVATKPVAAAPRVEEPKKVLTQRQGFKTNEFVVYPAHGVGQILA
                     IEEQEIAGAKLELFVINFMKDKMTLRVPTAKVANVGMRKLSEPALVKRALETLKGRAR
                     VKRTMWSRRAQEYEAKINSGDIVAIAEVVRDLYRSESQPEQSYSERQLYEAALDRLSR
                     EIAVVQHGTETEAVKEIEGQLAKSPRRGAKAETEVEADGEADAEADGDDAAVADEAA"
     gene            5816..6043
                     /locus_tag="VN968_27580"
     CDS             5816..6043
                     /locus_tag="VN968_27580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33028.1"
                     /translation="MVATTGFAATFATGFGAVLIGGVLIGGVLAGATFEAEGFEPVAF
                     AAVFAVLFGIALLLFFEDFAAEALVAFRVSL"
     gene            complement(6383..6721)
                     /gene="fdxA"
                     /locus_tag="VN968_27585"
     CDS             complement(6383..6721)
                     /gene="fdxA"
                     /locus_tag="VN968_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HXL33029.1"
                     /translation="MTYVVTEACIKCKYTDCVEVCPVDCFYEGDNMLVIHPDECIDCG
                     VCEPECPADAIKPDTEPGLEKWLEVNTEYAKTWPNITQKKESPADAKDFEGMEGKFEK
                     YFSPAPGTGD"
     gene            complement(6857..7126)
                     /locus_tag="VN968_27590"
     CDS             complement(6857..7126)
                     /locus_tag="VN968_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HXL33030.1"
                     /translation="MERQRLDKWLWHARVVKARTSAAALVEAGHVRINGVREKAPGHA
                     VKAGDVLTIGLDHSVRILKVVGFAERRGDASAARVLYDDLQERRE"
     gene            complement(7144..>10023)
                     /locus_tag="VN968_27595"
     CDS             complement(7144..>10023)
                     /locus_tag="VN968_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HXL33031.1"
                     /translation="VAMFQSGDVDYLVATDAVGMGLNLDVDHVAFASDRKYDGYQFRR
                     LNPSEFAQIAGRAGRATRDGTFGTTGRCAPFEPELVNALQNHSFESVKTLQWRHSKLD
                     FSSLGALQVSLALTPSHEALTRAPIAEDQRVLDHAARDADVREMAHGAAAVERLWDAC
                     QIPDYRKIAPAAHAELVTTLFDFLMQKGRIPDAWFAAQVEQADRVDGDIDTLSSRIAQ
                     IRTWTFVANRPDWLADPEHWQGIARDVENKLSDALHERLTERFVDRRTSVLMRRLREN
                     TMLNTEIGKTGEVIVEGHVIGRLDGFTFAPDAAEAGSDAKALQATAQKALAGEIDARA
                     EKLAAAPDDQFVLTSDATIRWTGDAVARLVAADDALHPRIRIISDDRLTGAPREAVQT
                     RLDLWLKAYIEKLLGPLFGLAKAEDITGIARGIAFQLIEALGVLERSKIAAEMKDLDQ
                     PSRATLRKYGVRFGAYHIYVPALLKPAARALASLLWAQKLDNVDLSALSGAQHLAGSG
                     RTSFPIDKLLDRDAYRVLGYRQCGERAVRVDILERLADLIRPALAWREGSPSQKPAGA
                     FDGRGFVVTQAMTSLTGSAGEDFASILRALGYRMERRPAPPKPAAIEATPVETAPLEV
                     AAPEASSPEAALPESAATDTSGLQPVAAAETADTADITNDVPPTVEATAVSSATLLPN
                     VAFSPASHEHEAAAVEANPIDVLPDPAASTENLTPETLASEAPPSEAPASETPVSEPP
                     QVEASAEIATESAAVGDAAPAAAPAAGATVPDVAAAPEMVDVWRPGGRSEERRPRHDR
                     GRHRHQGNQDRPQEGAQPVAATGEANDGARRERHGRGRRDRGNDFRKPRSDAPPVEAA
                     VAPAAEAAPVREPRDDKGRPSRERFADKGRDRDKDRDKGKFGGGRDKGGRDKGGRDKR
                     DGGPSHRQFATSAPPRERERPVDPNSPFAKLAALKEQLTANRKDR"
BASE COUNT         1779 a         3185 c         3144 g         1915 t
ORIGIN      
        1 ccctgcgcca gcgacatcgc aagttcatgc gccgccgctt cgacctcggc gtcgggcacg
       61 acttttgtca cgatgccgag tgcgaaggct tcctgcgctg acagcaccgg cgacatcaag
      121 taaagctccc gtgctttcgc actgccgagc agatgggtca ggaaataggt gccgccgtaa
      181 tcgcctgata gcccgacctt ggcgaaggcg gtggtgatct tgacggaagc agaggcgacg
      241 cggagatcgc aggccagcgc aatcgacaag cccgcgccgg cggccgcgcc atcgacctgc
      301 gccaccatgg gctttggcat gtcgtgcaga atccgggaga cttccatgcc gcgccggaga
      361 ttggccatcc tggcctcgaa cggcagcggc gcccggccct ccgccatcga tttcacatcg
      421 ccaccgacgc agaacgtgcc gccggcgccc ttgagcagaa ccgcgcggac ctcgtggtcc
      481 tccgccgcgc gacgcgccgc ttccaccaga ccccgcgtca tgtccggatt gagcgcgttg
      541 cgccgatccg gacgattcat ggtgatggtg agcaggccct ggtcgagttt ttggaggacg
      601 atgtcgttca tgagttttgc ctttgttttg ttattcgttg ttgttcattg ccgtctctgc
      661 gaagccatcc acagccagcc tcaggaagga tggattgctt cgttgcttac gctcctcgca
      721 atgacggcgt atgatcactt cttcaccagc ggacagcgtg acagttcgag cgactggaac
      781 gcctcattgc cggggatggt cgcgagcagc ttgtagtcgt cccagcggcc tttggattcc
      841 gacggcttct tgacctcgaa cagatacatg tcgtgcacca tgcggccgtc ctggcgaatc
      901 cgcccctgct tggcgaacac atcgttgatc ggggtgtcct tcatgatctt catgacggcg
      961 gccgagtcgg tggtgcctgc agccttcacc gccttcagat aatgcgtgac cgacgaatag
     1021 acgccgacct gggccgcggt cggcacgcgc ttgacgcgct ccatgaagcg ttttgagaaa
     1081 gcgcgagtgt cgtcgttgag gtcccaataa aacgcttcgg cgagcagcag tccctgcgcg
     1141 gtctcaagac cgacgctgtc gatatcggtg acgaaggcca gcaacggcga cagtttttgg
     1201 ccactcctgg tcagcccgaa ctcggcggcc tgcttgatcg cgttgatggt gtcgccgccg
     1261 gcattggcga gcccgatcac cttggctttc gagctttgcg cctgcagcag gtaggacgag
     1321 aaatccgagg tgttgagcgg gtggcggacg ctgcccagca ccttgccgcc ggacttcagc
     1381 acgacattgg tggtgtcttt ttccagatcc tgtccgaagg cgtagtcggc ggtgagaaag
     1441 aaccagctgt cgaggccctg cttgacggcg gcgagaccgg tgacgttggc ctgcgcgaag
     1501 gtgtcgaaca cataatgcac ggtataggga ccgcaggctt cgttgctgag gcggatcgat
     1561 cccggaccat tgaagatgat gatcttgttg cgcgtcttcg cgatctcgcc agcggcgagc
     1621 gcggtcgcgg aggccgccac gtcaaagatc atctcgacgc cctgattgtc gagcatgtcg
     1681 cgggcgatat tggcggcaag atcggccttg ttgagatgat cggccgcgat gatctcgatc
     1741 tttcggccca gcacctcgcc gccgaaatcc tcggccgcca tcttcgctgc gatcgcgctg
     1801 cccggcccgg tgatgtcggc gtacagaccc gacatatcga ggatgccgcc gagcttcagc
     1861 ggaggcttgc tttgcgccat ggccgcggtg gcggcaagca ccagcgccga tgcgagaaca
     1921 ccggacagta cgcggttcat tgcagctccc tcgcgaacgt gccgcattcg ttggcggcct
     1981 taatttctgt tgctgcgatc atgccgcaag cgatccgcag cggcaagcgc agtcacccgc
     2041 gcggccacgc accgcgcttt gagccctaag tattttccgc aaaacggaat gctcgctgcc
     2101 gcaacgccac cacgctcgaa tttgcatgcc gctcagctca aaagcccctt tcccggaaca
     2161 tggttgagct ccaacctgat gccgtcggga tcttcgaaca gcagcgaata atatcccggc
     2221 gcccactgat cctcgcgcgg cgcgcgaacg atcgttacac cgtgcgaaca cagaaagccg
     2281 tgcagctcgt caacgtcggc gcgctcgcgg gcgcgaaaac acagatggtg caggccgacg
     2341 cgattctgct cgaacgcagc gccggcgtgc tcggccgccg gcgcccggat cgcgaccgcg
     2401 gtgcggccgc cgacgcaata atagacggca tcggtatcga tgacaggctt cagcccaaga
     2461 aaaggcagca gcttgcggta gaattcgcgg gagcggtcga aattcgatgc ggtcaagaaa
     2521 atatgcgcga tgccgttgac ttccatcgac ttcccccttg tcagtgaaac gatatcggat
     2581 acggagcgcg ttggcagatg atcaacatac agaaattcga ccggacgtag aggttcgtca
     2641 tggaaccggc aagcagagcg gaaccgcggc gcggcggatt tgtcgcgata gcattgccgt
     2701 gcagcttgat gctcgtcgcc gccgcggccg gtccggccga cgcagccgga tgcgcgttcg
     2761 agccgcaggg cgagggacgc gtcgccgccg tgatcgatgc gcgcagcttt cgcctgcagg
     2821 atggtcgcga ggtccgcctc gccggcatcg agccggtcgc ggtcgaaaag gaccatgcca
     2881 gccgcatatc ggcgctggcg gccatcgtga tggggcgcga tgtgacgttg cgcggggaag
     2941 atgattcgcc cgaccgctac ggccgtcagc ccgccttcgt gtttctggat ggcaccgaga
     3001 cgtcggtgca gggcctgctg ctgacgcagg gcgaggcgtt ggtttcggcc accgtggcgg
     3061 acaaagactg tgccttgagc ctgttggccg cggaaacggc ggcgcgcgac gcaaaacgag
     3121 gcacctgggc cgatccagcc gccataaaaa acgcggaaag tccgggcgat atcttgaccg
     3181 ggatcgggcg attcacggtg gtcgagggca aggttttgtc ggtgcgacag gctggggcaa
     3241 cgacttacct aaatttcgga cggagctgga cacagggctt tgctgtgact atttcaaggc
     3301 gcgcgacagc ggcgcttgag attgccggaa tcatcgttaa gtcgcttgaa aatcgacgaa
     3361 ttcgtgtccg aggctgggtc gaggcgcgca gcggcccgcg gatcgaagtg ctccgcgcgg
     3421 gacagattga gttgctgggc gagaattagc tctctggaca aatacgagta gctgacgtgg
     3481 cagaatgcgc gggacggcac aagacctgga tgagccgccg cctttgggct gcgccgatgc
     3541 tgctgtgcgc ggcctttgcg ctttccgcct gcagcgatgt cgccaagttt caaaccccga
     3601 tcccgaccgc aagcccgctc aaggcccgga cagttgcgca aaccccggcc gccgagcgcg
     3661 agcacgaacg gatcctgtcc tcctatggcg gttcctatga cgatccgaac ctggaggccc
     3721 tgatcggcaa gacggtcgac cggctggttg cggcatcgga ccgtcccgac caggcctaca
     3781 aggtgacgat cctcaattct ggcgcggtga atgcgttcgc gcttccgacc ggacagcttt
     3841 acgtgacgcg tggcctggtc gcgctggcca gcgatacctc ggaattgtcc tcggtgctga
     3901 gccacgagat ggcccatgtg ctggctaaac acgcggcgat ccgcgaggac caggcgcgtc
     3961 aggccgccgt cgtcacccgc gtcgtcaccg acatgggcaa cgatcccgat ctcaccgccc
     4021 ttgcgctcgc caaaaccaag ctgacgatgg cgagcttttc gcgcgcgcag gaattcgaag
     4081 cggacgccat cggtgtcagt atttcggcgc gtgcccattt cgatccctac ggcgcggcgc
     4141 gtttcctgac gtcgatggag cgcaatgccg agatgaaggc gggaaagacc tcgctcgatc
     4201 cgcgcgccca ggattttctg tcgtcgcacc cggcaacgcc ggagcgcgtg gagaacgcgc
     4261 aggcgaccgc aaggcaatac acctcgccgg cgagcggtga acgcgatcgc gagacctatc
     4321 tggccgcgat cgacaacatc gtctatggcg aagacccaag cgaggggttc gtccggggcc
     4381 gccgcttcct gcatcccaag cttggcttca cgttcgccgc ccccgaaaac ttcacgctcg
     4441 acaataccgc gcaggccgtg atcggggtgc gcgagggcgg tacccaggcg atgcgcttcg
     4501 atgtggtaag agtgccggcg gaacaatcgc tcggcgacta tctcaattcc ggctggatgg
     4561 aaaatgtcga caagccgtca accgaggatc tgacgatcaa cggcttcccg gcggcgtcag
     4621 ctcaggcgcg cggcgaacaa tggcagttca gggtctacgc gctgcgcttc ggcagcgacg
     4681 tctaccgctt cattttcgcc accaaggaaa agaccaccga gagcgaccgc aacgcgcgtg
     4741 aaaccgtgaa ttccttccgc cgattgtcgc tggaggaaat tcaggcggcg cgaccgctgc
     4801 gaatcaagat catcaccgcg caacccggcg ataccgtcga atcgctgtcg caccgcatgg
     4861 ccgcggtcga ccggccgatg gagcgctttc gcgtcctcaa cggcctcgac gcccatgccc
     4921 aggtcaaggt gcgcgatctc gtgaagatcg tggtggattg atccaccatc gtcgtcccgg
     4981 cgaacgccgg gacccatgat caccgacgtc ggttgtcgaa gaagggcttc tgctccaatg
     5041 ccagaacgaa agtgcacggc gtatgggtcc cggccttcgc cgggacgacg tattgataga
     5101 cttcgctgaa tcaccaacag caaaaagccc ggtctcatcg accgggcttt tgaacgttgg
     5161 atctccgagg ctgccttacg ccgcctcgtc cgctaccgct gcatcgtcgc cgtcggcttc
     5221 ggcatcggct tcaccgtccg cttcgacttc ggtctccgcc ttggcgccgc ggcgcgggct
     5281 cttggcgagc tggccttcga tttccttcac cgcctcggtt tccgtcccgt gctgcacgac
     5341 ggcgatttcc cgcgacaggc gatcgagcgc cgcttcatag agctgacgtt cgctgtagga
     5401 ctgttcgggc tgcgattcgg agcgatacag atcgcgcacc acctcggcga tcgcgacaat
     5461 gtctccggag ttgatcttgg cctcatattc ctgcgcccgg cgcgaccaca tcgtgcgctt
     5521 gacgcgcgct cggcccttca gggtttccag cgcgcgcttg accaatgccg gttccgacag
     5581 cttgcgcatg ccgacattgg caaccttggc ggtgggcacc cgcagcgtca tcttgtcctt
     5641 catgaaattg atgacgaaca gttcaagctt ggcgccggcg atctcctgct cctcgatcgc
     5701 caggatctgg ccaacgccat gggcgggata caccacgaat tcgttcgtct tgaagccctg
     5761 acgctgggtc aaaaccttct tcggctcttc gacccgcggc gcggcagcca ccggcttggt
     5821 ggcgaccacg ggcttcgccg caaccttcgc gacgggcttc ggagcagtct tgattggggg
     5881 agtcttgatt ggaggagtct tggcgggagc aaccttcgaa gcagagggct tcgaacccgt
     5941 cgctttcgcg gcagttttcg cagtcttgtt tggcattgcg cttcttttgt ttttcgagga
     6001 ttttgcagcc gaggccctgg tggccttccg ggtatccctt tgagaagacc ggacgcggct
     6061 tttggttgcg ccacggctgc ccgcagcaga tttcttagaa tctttggaag cactcttttt
     6121 acgcgttttc tgtgacacag cctgcgcgcg gaactgccac gcccctgttc gatgttttca
     6181 cgggaacccc ctgaggccac acggctgggt tcggcttgaa tatggccaat atagcacatt
     6241 ttcggtgaaa atcaatgatt tacggcccgt tcggggctcg gcggggtgat ttcgccgata
     6301 tcaaaggtat tagggttaag ccgcttgtcg ttgaataagg ccaaaaatgg ccgccgctca
     6361 agccgataaa cgccgtaacg gatcagtcgc cggtcccagg agccggagaa aaatatttct
     6421 cgaatttccc ttccataccc tcaaaatcct tggcgtcggc cggggattcc ttcttctggg
     6481 tgatgttcgg ccaggtcttg gcatattcgg tattcacctc gagccatttt tccaggccag
     6541 gctcggtatc gggcttgatg gcgtcggcgg gacattctgg ctcgcacact ccgcagtcga
     6601 tacattcatc gggatggatc accaacatgt tgtcgccttc gtagaagcaa tctacggggc
     6661 agacctcaac gcaatcggta tatttgcatt taatgcaggc ttcagtgacg acgtaagtca
     6721 tccaaggctc cggaacgggt caattttcag tggtttgcgt agcgcaggaa ctgcgtagtc
     6781 gcaagtgacg cagaacactg aagagcggtg ttttcaggcg cttcgatgcg agctgaattc
     6841 gcccgaatac agatagttac tctcgccgtt cctgcaaatc gtcgtacagc acccgcgcgg
     6901 cagacgcatc gccccgccgc tcagcaaatc cgacgacttt gaggatgcgc acgctgtgat
     6961 caagcccgat cgtcagcaca tcgccggcct tgacggcatg gcccggcgcc ttctcgcgca
     7021 cgccattgat gcggacatgg ccggcctcga ccagcgcggc ggcgctggtg cgggccttca
     7081 ccacccgcgc atgccacaac catttatcga gacgctggcg ctccaagcaa caccgattac
     7141 gctttagcga tccttgcggt tggcggtcag ctgttctttc agcgccgcca gtttggcaaa
     7201 cggcgaattg ggatcgaccg ggcgttcccg ttcgcgcggc ggggccgagg tcgcgaactg
     7261 ccggtgcgac gggccaccat cgcgtttgtc acgcccaccc ttgtcgcggc cgcctttgtc
     7321 gcgcccaccc ccgaatttgc ccttgtccct gtccttatcc cggtccctgc ccttgtcggc
     7381 aaagcgctcg cgggaggggc gacccttgtc gtcgcgaggt tcgcgaaccg gtgcggcttc
     7441 cgctgccggt gccacggccg cttcgaccgg cggcgcatcg ctgcggggtt tacggaaatc
     7501 gttaccgcgg tcgcgccgtc cgcggccgtg gcgctcgcgc ctggcaccgt cgttagcctc
     7561 gcccgtggcg gcaaccggct gagcgccttc ctgcggacgg tcctgattgc cctgatggcg
     7621 atgacggccg cgatcatggc gcggccggcg ctcttccgac cggccgccgg gccgccagac
     7681 atccaccatc tccggagccg cagcgacatc gggcacggtc gctcccgcgg caggcgccgc
     7741 cgccggcgcg gcgtcgccaa cggcagcgct ttcggtcgct atttcggctg atgcctcgac
     7801 ctgcggcggt tccgacaccg gcgtttccga agccggagct tctgacggcg gagcttctga
     7861 tgccagggtt tccggcgtca ggttttccgt cgaagccgcg ggatccggca aaacgtcgat
     7921 cggattggct tcgactgccg cggcctcatg ctcatgcgaa gccggcgaga acgcgacgtt
     7981 gggaagcaac gtcgccgacg ataccgcagt cgcttcaacg gtcggcggca catcgttggt
     8041 gatatcagcg gtatcggccg tctcggcggc ggccacgggc tgcaacccgc tggtgtcggt
     8101 agctgcgctc tccggcaacg ccgcttccgg agacgacgcc tctggcgcgg cgacttcaag
     8161 cggcgcggtt tccacgggcg ttgcttcgat cgcagccggc ttcggcggcg ccgggcggcg
     8221 ctccatgcga tagccaagcg cgcgcagaat cgaggcgaaa tcttcgccgg ccgacccggt
     8281 aagagaagtc atcgcctggg taacgacaaa accgcggccg tcgaacgcgc cggcaggctt
     8341 ttggcttggc gaaccctccc gccatgccag cgcggggcgg atgagatcgg cgaggcgctc
     8401 cagaatatcg acccgcaccg cgcgctcgcc gcattggcga tagccaagca cgcgataggc
     8461 atcgcggtcg agcagcttgt cgatcgggaa cgaggtgcgg ccgctgcccg caaggtgctg
     8521 cgcgcccgac agcgccgaaa ggtcgacatt gtccagcttc tgcgcccaca acagcgacgc
     8581 cagcgcgcgc gccgccggct tcaggagcgc cggtacatag atatgatagg ccccgaaccg
     8641 cacgccatat ttgcgcaacg tggcccgcga cggctgatcg agatccttca tctcggcggc
     8701 gatcttcgaa cgctccagca cgcccagcgc ttcgatcagc tgaaaggcga tgccgcgcgc
     8761 gatccctgta atgtcctcgg ccttggcgag gccgaacagc ggtcccaaga gcttttcgat
     8821 gtaggccttc agccacagat cgagccgtgt ctgcacggcc tcacgcggcg cgccggtcag
     8881 gcgatcgtcg gaaatgatgc ggatgcgcgg atgcagcgcg tcgtcggcgg caaccagtct
     8941 tgcgacggcg tcgccggtcc agcggatggt ggcgtccgat gtcagcacga actgatcgtc
     9001 gggtgcggcc gccaatttct cggcgcgcgc gtcgatttcg ccggccagcg ccttttgcgc
     9061 ggtcgcctgc agcgctttgg catcggaacc ggcctcggcc gcatcgggcg caaatgtgaa
     9121 accgtcgagg cggccgatca catggccctc tacgatcact tcgccggtct tgccaatttc
     9181 cgtattcaac atcgtgtttt cccgcaggcg gcgcatcaat acactggtac ggcgatcaac
     9241 gaaacgctcc gtgagccgtt catgcagcgc atcggacaat ttattttcaa catcgcgtgc
     9301 aatcccctgc caatgttcgg gatcggccag ccaatccggg cgattggcca caaaggtcca
     9361 ggtgcggatt tgcgcgatcc ggctcgatag cgtgtcgata tcgccatcga cgcgatcggc
     9421 ctgctcgacc tgggcggcga accaggcgtc cggaatcctg cccttttgca tcagaaagtc
     9481 gaacaaagtc gtcaccagct ccgcatgcgc ggcgggcgcg atcttgcggt aatccggaat
     9541 ctggcaggcg tcccacagcc gttccaccgc ggccgcacca tgcgccatct cgcgcacatc
     9601 ggcatcgcgt gcggcgtgat cgagcacgcg ttgatcctcc gcgatcggcg cgcgggtcag
     9661 cgcctcgtgg gatggcgtca atgccaaaga cacctgaagc gcgccgagcg acgaaaaatc
     9721 cagcttcgaa tggcgccatt gcagtgtttt tacgctctcg aagctgtgat tctgcagcgc
     9781 gttgaccagt tccggctcga acggcgcgca gcgcccggtt gtgccgaacg tgccgtcgcg
     9841 ggtggcgcgg ccggcgcggc ccgcgatctg ggcgaattcg gacggattga ggcggcggaa
     9901 ctgatagccg tcatacttgc ggtcggaggc gaacgcgacg tgatcgacgt cgaggttgag
     9961 ccccatgccc accgcatcgg tcgcgaccag gtaatcgacg tcgccggact ggaacatcgc
    10021 cac
//
LOCUS       DATNOI010000689         2463 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_441990,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000689 DATNOI010000000
VERSION     DATNOI010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441990"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VN968_27600"
     CDS             complement(<1..187)
                     /locus_tag="VN968_27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXL33032.1"
                     /translation="MAIVNRVADLQPDIQAWRRDIHQHPELLYDVHRTAAFVADRLKE
                     FGCDEVATGLGRTGVVGV"
     gene            430..2049
                     /locus_tag="VN968_27605"
     CDS             430..2049
                     /locus_tag="VN968_27605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXL33033.1"
                     /translation="MPKRLEGDFDYIVVGAGTAGCIVANRLSADPRNRVLILEAGGND
                     NWIWFHIPVGYLFAIGNPRSDWMFRTEPEAGLNGRSLAYPRGKVIGGSSAINAMISMR
                     GQAADYDHWRQLGLGGWGWDDVKPAFRRLEDHFLGESEHHGTGGGWRIEAPRLSWIVL
                     DAVGKAAEEMGIPKIPDFNTGDNEGVGYFHVNQKRGRRWSSARGFLKPALRRPNLHLE
                     KDVLVDRLIIEQGRAVGVRFIQTGEVAEARARGEVILCAGSIGSIQVMHRSGIGPADW
                     LAPLGIETIHDRQGVGRNLQDHLQQRAIYKVEGVRTLNETYYSLVRRGLMGLDYAFRR
                     RGPLTMAPSQLGIFTRSDPHRERANIQFHVQPLSLDKFGDPLHRFPAITVSACNLQPT
                     SRGTVRIRSTQADEAPSIAPNYLSTAEDRQVAADAIRTTRRLMKQPALARYRPEEFLP
                     GPAVGDDDASLAQAAGDIGTTIFHPVGTAKMGTANDPMAVVDERLRLYGIAGLRVADA
                     SVMPTITSGNTNTPTAMIAEKGAAMILEDSR"
     gene            complement(2085..>2463)
                     /gene="hpnO"
                     /locus_tag="VN968_27610"
     CDS             complement(2085..>2463)
                     /gene="hpnO"
                     /locus_tag="VN968_27610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminobacteriohopanetriol synthase HpnO"
                     /protein_id="HXL33034.1"
                     /translation="ALTRMVPGYELLKEVRGKGLMIGVEFGPPKSLRLKASWNVLETA
                     NKGLFCQLITVPLFKDHKILTQVSGHGSHTIKLLPPLTITEEDCNWIETAFDSVIASS
                     HKVPGAIWSLGKTLVDNAIRKSA"
BASE COUNT          465 a          772 c          775 g          451 t
ORIGIN      
        1 tgacgccgac gacaccggtg cggccaaggc cggtcgccac ctcgtcgcag ccgaactcct
       61 tcaaacggtc cgccacgaat gccgcagtgc ggtgcacgtc gtacagcaat tcgggatgtt
      121 ggtggatatc ccggcgccaa gcctggatat cgggttgcag atcggcgacg cggttaacga
      181 tggccatggc aattctcggt tacggggttt gagcgtttta tcatctctag cacgcacccg
      241 cgatccgccc aacactcctg cggcagtagc ccaagtgtca gccatgtctc ccgaacaggc
      301 gtcggctatg tcccccaaca gaacgcttgt cctgggcatc cgcgtctgac tgaataaaac
      361 cagcaagacg tcacgcccgg catgaagccg gacatgacga caaatcatgg attgggcgga
      421 aactcaggaa tgccgaagcg gctagaaggt gatttcgact atatcgtcgt cggcgcgggc
      481 accgcgggct gcatcgtcgc caaccggctc tcggccgatc cgaggaaccg cgtgctgatc
      541 ctggaagccg gcggcaacga caactggatc tggtttcaca tcccggtcgg ttatctgttc
      601 gcgatcggta atccgcgttc ggactggatg ttcaggaccg agccggaagc cggtctgaac
      661 ggccgctcgc tggcctatcc ccgcggcaag gtgatcggcg gttcatccgc gatcaacgcc
      721 atgatctcca tgcgcggaca agccgccgat tacgaccact ggcgccagct tggccttggc
      781 ggctggggtt gggacgacgt caagccggcg ttccggaggc tggaggatca ttttctgggc
      841 gaaagcgagc atcatggcac gggcggcggc tggcggatcg aagccccgcg gctgtcctgg
      901 attgtcctgg acgccgtcgg caaagccgcg gaagagatgg gcattccaaa aattccggat
      961 ttcaacaccg gcgacaatga gggcgtcggc tatttccacg tcaaccagaa gcgcggacgc
     1021 cgctggtctt ccgcgcgagg ttttctgaag cccgcgctga ggcgccccaa tcttcacctc
     1081 gagaaagacg tgctggtgga tcgcctgatc atcgagcagg gccgcgcggt cggcgtgcgc
     1141 ttcattcaga caggcgaagt cgccgaggcc cgcgccaggg gcgaagtgat cctgtgcgcc
     1201 ggctcgatcg gatcgataca ggtaatgcat cgatcaggca tcggcccggc cgactggctc
     1261 gcgccgcttg ggatcgagac gatccacgac cggcaaggcg tggggcgcaa tctgcaggac
     1321 catctgcagc agcgcgcgat ctacaaggtc gaaggcgttc gcacgctcaa cgagacctat
     1381 tattcgctgg tgaggcgcgg cctgatgggt ctcgactatg cgttccgccg ccgtgggccg
     1441 ctgaccatgg cgccctcgca gctcggcatt ttcacccgtt cggatccgca tcgcgagcgc
     1501 gccaatatcc agttccacgt gcagccgttg tcgctggaca agtttggcga tcccctgcac
     1561 cgcttcccgg cgatcacggt gagcgcctgc aatttgcagc cgacctcgcg cgggaccgtg
     1621 cgcattcgct cgacgcaggc cgacgaagca ccttcgatcg cgccgaatta tctatcgacc
     1681 gccgaggacc gccaggttgc cgccgatgcc atccgcacca cgcggcggct gatgaaacag
     1741 ccggcgctgg cgcgctatcg ccctgaagaa tttctgccgg gcccagcggt gggcgacgat
     1801 gacgcgtcgc tggcgcaagc cgcgggcgat atcggcacca cgatctttca tcccgtgggc
     1861 acggccaaga tgggcacagc caacgatccc atggcggtcg tcgatgaacg gctgcgtctt
     1921 tatggcattg ccggacttcg cgtcgccgac gcctcggtga tgccgacgat aacttccggc
     1981 aataccaata cgccgaccgc gatgatcgcg gaaaaaggcg cggcgatgat tctcgaggat
     2041 tcgcgataga gcgcggtcgc cggacaagcg aagccgcttg tccgtcaagc cgatttgcgg
     2101 atggcgttgt cgaccagtgt cttgcccagc gaccagatcg cgccgggaac cttgtggctg
     2161 gaggcgatga cggaatcgaa cgccgtctcg atccagttgc aatcctcttc cgtgatggtc
     2221 agcggcggca acagtttgat ggtgtggctg ccgtgacccg acacctgggt caggatcttg
     2281 tggtccttga atagcggcac cgtgatgagc tggcagaaca gacccttgtt ggcggtttcg
     2341 agcacattcc acgacgcctt gagccgcagc gattttggcg ggccgaactc gacaccaatc
     2401 atcagcccct tgccgcgaac ctccttgagc aattcatagc cgggaaccat tcgggtcagc
     2461 gcc
//
LOCUS       DATNOI010000690         8250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_881424,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000690 DATNOI010000000
VERSION     DATNOI010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881424"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VN968_27615"
     CDS             complement(<1..958)
                     /locus_tag="VN968_27615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL33035.1"
                     /translation="MSKDDVVRTSDLLRPRHVGMPIRRTEDPRLLTGNGEYTADRKPD
                     RALHVAFRRSEQPHATILRIDATQALTAPGVVAVFTADDVAEDCKPVIPFSRMPNYYA
                     TPITALASKKVRYVGEAVVAVIAEDRYLAEDALELIEIEYAPLGAVTDPEYAASEDAP
                     RLHEEAATNVLIAREFRKGDAAADLAAAHVRVGDRFEMTRKAGLAIEPRSYCAEFEGR
                     RDSITLYTSSNIPGIVRDALAESLDLPGSRLRVVAPDVGGSFGSKGSLYPEEILLCVA
                     AKKLRRSLKWTADRLEDISSSSQAFAEIVDAEMGFDENGMAIA"
     gene            complement(951..1712)
                     /locus_tag="VN968_27620"
     CDS             complement(951..1712)
                     /locus_tag="VN968_27620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004674371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HXL33036.1"
                     /translation="MKLQFLSAGRLRLKKSVYIRGADRNETFEAPVISALIRHKQGNV
                     LFDTGCHPSVVEHGEERWGSLMKVMTPVMRAEDTLLPNLACVGVNPDDIDVVVSSHFH
                     PDHCGCNQFFRKATILAHAREIEAAKAAGAEAAGYLQADWDHGQPMDPVNGERDVFGD
                     GKLVLIPLPGHTPGTLGAAVHLDRDGAFLLASDAVSLRENLDSDTSPRNTWNAEALLN
                     SYREIRRIENSGATVICGHDDAQWQSLRKGEEAYE"
     gene            complement(1709..2182)
                     /locus_tag="VN968_27625"
     CDS             complement(1709..2182)
                     /locus_tag="VN968_27625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL33037.1"
                     /translation="MDIVVTVNGRKYQASIEPRLLLSDFLRDTLGLTGTHVGCEHGVC
                     GACTILVNGDSVRSCLTLAVRTDGMDIVTVEGLGTRDHLNALQSEFREKHGLQCGFCT
                     PGMLMTAMDLLKKYPLASDEQIREGLSGNLCRCTGYQNIVAAIRSAVSLRNGSVS"
     gene            complement(2185..3057)
                     /locus_tag="VN968_27630"
     CDS             complement(2185..3057)
                     /locus_tag="VN968_27630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HXL33038.1"
                     /translation="MKPAAFDYIAPNSLEAAVEAIAAANGDGKILAGGQSLMPLLNFR
                     MARPSVIVDLMHIPDMSFIERRGDTVAIGALTRHADLEFSDLIAQKLPVMAAAMPHVA
                     HLAIRNKGTIGGSLSHADPAAELPMLAVFYGATIKVQGSGGRRDIPAEDFFVSALTNC
                     LDPDEIVFEIDFPVLTSHAGWAFEEVARRFGDFALASIAVSLGILDNRIVDARVAVMG
                     VADTPRRLREAEDALRGEGEPKTAAKFGKIVRACVSPESDIHVSAEYRKNLIGALAER
                     AFTTAWTRAVGAKP"
     gene            complement(3117..3875)
                     /locus_tag="VN968_27635"
     CDS             complement(3117..3875)
                     /locus_tag="VN968_27635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXL33039.1"
                     /translation="MTTRDSIGVVSLNRPQMRNAVTLGMWRELAGIFSSLADDDAVRA
                     IVRTGSGADFCVGADVSEFGAIRENRDQSAEYEVSVDACSAAIANVSKPVIAAISGYC
                     LGGGCHLALACDFRFADHTAKLGIPSARLSIVYGMNSVQRLLAIAGLANARRMLYSGE
                     RYDAARALSMGLVDEISDDAVSAALAFAGTLAGNAPLSIAGAKFMLNGLSMGEGALDI
                     AAAQRMIDAAADSEDFREGRRAFAEKRPPRFRGR"
     gene            complement(4017..4940)
                     /locus_tag="VN968_27640"
     CDS             complement(4017..4940)
                     /locus_tag="VN968_27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL33040.1"
                     /translation="MVGHAGGTSPLNALDDVNFDVMPGETLGLVGESGSGKSTAGRIL
                     VGLIPATAGTVSLFGRRITGRDGAAALGAVRSRLQFVFQDPYAALNPRMRVGTSVAEP
                     LEIAGKLSRRDRSDRVAELFELVGLPKISTDRYPHEFSGGQRQRIVIARSLALNPDFI
                     VCDEPVSALDVSMQAQIVNLLMDLQQRLGLTYLFIAHDLAVVRMISTRVAVMYAGSIV
                     ETAPKKDIYAQPQHPYTKALLDAVPRPDPAFVRAPVISGEVPSLLDPPQGCRFHPRCP
                     LVMDRCRREVPRLAASGTDRQVACHLFAERN"
     gene            complement(4981..5976)
                     /locus_tag="VN968_27645"
     CDS             complement(4981..5976)
                     /locus_tag="VN968_27645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL33041.1"
                     /translation="MAAAPLLAIRDLSVQFRVDAGWVTAVEGVSIDIGEGDCVGVVGE
                     SGSGKSVTALSILRLHDRATTRIPSGSIVFKGQDLLKSSKLSLRGIRGGEIAMIFQDP
                     MSSLNPVLTISEQISETIRQHQKMSGTAARRRAVELLDLVRIPDARRRADDYPHRLSG
                     GMRQRVMVAMAIACRPRLLIADEPTTALDVTIQAQILELLRELRKELGMSVMLISHDL
                     GVIAEFAQSVVVMYAGRVVEQAPVAQLFRRPVHPYTVGLMAAVPKLDGDATRLSAIPG
                     RIPGPQENIPGCRFAPRCGFVQNSCLQTPPPIEPAGELHTVRCPPQVLALGDVNG"
     gene            complement(5978..6868)
                     /locus_tag="VN968_27650"
     CDS             complement(5978..6868)
                     /locus_tag="VN968_27650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014489672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL33042.1"
                     /translation="MTDIGLEAIVSTAGLAPPRRGRLIRRLFRHRSFVIGFVIIVVLA
                     LAAVFAPLLTSLDPSAMKVRFRFRSPASDFIFGTDMFGRDIFTRVLYGARVSLFVGLA
                     VSVISGFCGAVLGVVAAQFRRLDAPIMRLMDALMSFPAILLALGIAAALGPRVSSVIA
                     ALTVAYVPAGVRIVRAAALVVREMDYVQAARLSGASALRIIVRHILPNCFSPLLVHLT
                     FIFAYAILAEAALSFLGVGVQPPLASWGNIIAEGRDYATEAWWVMLFPGIAISLAALG
                     MNLLGDALRDVLDPRLKGGD"
     gene            complement(6861..7805)
                     /locus_tag="VN968_27655"
     CDS             complement(6861..7805)
                     /locus_tag="VN968_27655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL33043.1"
                     /translation="MLRIIAFKFLQAVPVILIISILAFLLLSLLPGDPAIIIAGEQAS
                     PEAVEHVRQQLGLDRPFLQQLAIWLLNLAQGNFGSSLILNQSVLSAVGERLPVTLSLA
                     ALSIAVTIPVGVFLGSLAAYYRQSWIDAGVMTFALLGVSIPAFWIAILGIILFSVRLG
                     WVPSSGFTPISQGVGPWLSSVILPALILSLFQIGFLARMTRSAMLDVMGQDFIRTARA
                     KGLSEWRTVGKHALRNALILIITATGILLSTAIGGSVVIEQVFALPGIGRMVVQAILA
                     RDYPLVQGTMLIFGFTFVLINLVVDVLYTLADPRVRHD"
     gene            complement(7845..>8250)
                     /locus_tag="VN968_27660"
     CDS             complement(7845..>8250)
                     /locus_tag="VN968_27660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL33044.1"
                     /translation="VAEELKAAGINVDVQVTDWTTNAAHMQQGTGDWNLSTTAFGPDH
                     VLGPQQWRPMLYTFPHITGNDALDKAYGEFFAAPTLDQRRAAWLKIQQQVLGGANLIK
                     IADTGRLSGYAKKLKGQSDYAGVLQLWDLWLE"
BASE COUNT         1696 a         2624 c         2498 g         1432 t
ORIGIN      
        1 gcgcgatggc catcccgttt tcatcgaaac ccatttcggc gtcgacgatt tcggcgaagg
       61 cctgactgga actgctgata tcctcgagcc gatccgcggt ccatttcagc gaacggcgca
      121 actttttcgc cgccacgcat agcaaaatct cctcgggata gagcgagccc ttggatccaa
      181 aactgccgcc gacatccggc gcgaccaccc gcaaccggct gcccggcaga tcgagcgatt
      241 ccgccagcgc gtcgcgcacg atgccgggta tgttggacga ggtgtagagg gttatcgaat
      301 ctcttctgcc ttcaaactcg gcgcaatagc tgcggggctc gatcgcgagc ccggcctttc
      361 gggtcatctc gaaacgatcg ccgacacgga catgcgcagc cgcaagatcc gcagccgcat
      421 cccccttcct gaactctctg gcgatcaaca cgttggtcgc ggcttcctcg tggaggcgcg
      481 gcgcgtcctc tgaagccgcg tattcgggat cagtcaccgc gcccaacggg gcatactcga
      541 tctcgatcag ttccagggcg tcctcggcga ggtaccgatc ctccgcgatg acggccacca
      601 cagcttcccc gacatagcgc accttcttcg aggccagggc cgtaatcggc gtcgcgtaat
      661 agttcggcat gcgagagaac gggatgaccg gcttgcagtc ctctgccacg tcatcggcgg
      721 tgaaaaccgc cacgacaccc ggcgccgtca gcgcctgggt cgcgtcgatg cgcaggatcg
      781 tcgcgtgagg ttgctcgctg cggcggaaag caacgtggag cgcgcgatcg ggcttgcgat
      841 cagcggtata ttcgccgttg cctgtcaata gccgcggatc ttccgtccgc ctgatcggca
      901 tgccgacatg gcgcggtcgc agcaggtctg acgtccggac gacgtcatcc ttactcatag
      961 gcttcttccc ccttgcgcag gctctgccat tgagcgtcat catggccgca gatgaccgtt
     1021 gcgccggagt tctcgatccg gcgtatttcg cggtaggagt tcagcaacgc ttccgcattc
     1081 caggtattgc gcggcgacgt atcggaatcg aggttttcgc gcaggctcac cgcatctgac
     1141 gcgagaagaa aagccccgtc gcgatcgagg tgaacggcgg cgccgagcgt acccggcgtg
     1201 tgtccgggca gcgggatcaa taccagtttg ccgtcaccga acacatcccg ttcgccgttc
     1261 acgggatcca tcggctgtcc atggtcccaa tcggcctgca gatagccggc agcttccgcg
     1321 ccggccgctt tcgcggcctc gatctccctg gcatgcgcca ggatggtcgc tttcctgaaa
     1381 aactgattgc agccgcaatg gtcggggtgg aaatgcgaac tgacgacgac atcgatatcg
     1441 tccgggttga ccccgacgca ggcaaggttt ggcagcaggg tatcctccgc gcgcatgacc
     1501 ggtgtcatga ctttcatcag cgagccccat ctttcttcgc cgtgctcgac gacggaggga
     1561 tgacacccgg tgtcgaacag cacattgccc tgcttgtgcc ggatcagcgc gctgatcacg
     1621 ggcgcttcaa atgtctcgtt tcgatcggcg cccctgatat agacgctctt cttcaagcga
     1681 agccggccag cggaaagaaa ctgcagtttc atgaaacgct tccgtttcgc aagctgaccg
     1741 ctgagcgaat cgccgcgacg atattctggt aaccggtgca gcggcaaagg ttgccggaca
     1801 gtccctcgcg gatttgctca tcgctggcca gtggatattt tttcaagaga tccattgccg
     1861 tcatcagcat tcccggcgtg cagaagccgc actgaagacc atgcttttcc cgaaattcgc
     1921 tctggagggc gttcagatga tcccgcgtgc ccagaccttc aaccgtcaca atatccatgc
     1981 catcagtcct gacggccaat gtcaggcagg aacggacgct gtcgccattc accaggatgg
     2041 tgcatgcgcc gcatacgcca tgctcgcagc cgacatgcgt gcccgtcagc ccgagggtgt
     2101 cgcgcagaaa atcggaaagc agcaggcgcg gctcgatgct cgcctgatat tttcgtccgt
     2161 tgacggtgac gacgatgtcc atcttcacgg cttcgctccc actgcgcgcg tccatgctgt
     2221 ggtgaaagct cgctcggcca gcgcgccgat cagattcttg cggtattcgg cggaaacatg
     2281 gatgtcgctt tcgggtgaaa cgcaggcgcg gacgattttt ccaaattttg ctgccgtttt
     2341 gggctcgcct tcgccacgca gcgcgtcctc cgcttcgcgg aggcgccgcg gcgtatccgc
     2401 gacccccatg accgcgaccc tggcgtcgac aattctgtta tcgagaatcc caagcgatac
     2461 cgcgatgctc gccagcgcaa aatcgccaaa acggcgcgcg acctcctcga acgcccagcc
     2521 ggcgtgcgat gtcaggaccg gaaaatcgat ctcgaagacg atttcgtccg gatcgaggca
     2581 attggtaagg gcggatacga aaaaatcttc ggccggaatg tcccgccgac cgcctgagcc
     2641 ttgcaccttg atcgtggccc cgtagaaaac cgccagcatc ggcaattccg cggccgggtc
     2701 ggcgtgcgac aggctgcccc cgatcgtacc cttgttgcgt atcgccagat gcgcgacatg
     2761 cggcatcgcg gcggccatga ccggaagttt ttgcgcgatg agatccgaga attccagatc
     2821 ggcatgccgc gtcagagccc cgatcgcgac cgtgtcgccc ctccgctcga tgaacgacat
     2881 atcggggata tgcatgagat cgacgatgac agacggacgc gccatccgaa aattgagcaa
     2941 gggcatcaga gattgcccgc cggccaatat cttgccgtcg ccattcgcag ccgcgatagc
     3001 ctcgaccgct gcttcaaggc tgttcggagc gatatagtcg aaggcggcag gcttcattct
     3061 gcttctccgg aaagcgcgct cgacctcacg cccgctcttc caccgacgcg cgacattcag
     3121 cgtccgcgga accggggcgg ccgcttctcg gcgaaagcgc ggcggccttc cctgaaatcc
     3181 tcgctatcgg cggcggcgtc gatcatgcgc tgggcggcgg caatatcgag ggccccctcg
     3241 cccatggaca atccattcag catgaatttc gctcccgcga tcgacagcgg cgcgttgccg
     3301 gcaagtgtgc cggcgaatgc gagggcggcc gagaccgcgt catcgcttat ctcatcgacg
     3361 aggcccatcg acagcgcgcg cgccgcgtcg tagcgctcgc cggaataaag catgcgcctg
     3421 gcgttggcta aacctgcaat cgccagcagg cgctggacgc tgttcatgcc atacacaatc
     3481 gaaagcctgg cagacgggat tccaagtttg gcggtgtggt ctgcgaagcg aaaatcacaa
     3541 gcgagcgcca gatgacagcc gccgccaagg caatagccag agatcgctgc gatcaccggc
     3601 ttgctcacat tcgcgatcgc ggccgagcag gcatcgaccg aaacttcata ttcggcgctc
     3661 tgatcccggt tttccctgat cgcgccgaac tccgacacgt cggcgccgac gcagaagtca
     3721 gcacccgacc cggtccgaac gatcgcgcga accgcatcat cgtcggcaag gctcgaaaat
     3781 atgcctgcaa gttcgcgcca catgccaagc gtcacggcat tgcgcatctg cggccggttg
     3841 aggctgacaa ctccaatgct atcccgtgtg gtcacgagga tatctggcat ttcccaccct
     3901 cggccgatgt cgcggttggt ctcactgccg cgttacttgc ataagtagac ttacaaagtg
     3961 tccttcgtca agcgccagct cgcgggcatt catccgccct cggataataa aatgcctcag
     4021 ttcctttcag caaacaaatg gcaggcgacc tgacggtctg ttccggacgc tgcaagcctg
     4081 gggacttccc tgcgacagcg atccatcacc agcggacagc gcgggtggaa ccggcagccc
     4141 tggggcggat cgagcaggct cgggacttcg ccggatatga ccggcgcgcg gacaaacgcc
     4201 ggatccggtc gcggcaccgc atcgagcagc gccttcgtat aggggtgctg cggctgcgcg
     4261 tagatgtcct tcttcggcgc ggtttcgacg atggagcccg catacatcac cgcgacgcgg
     4321 gtcgagatca tccgaaccac cgcgagatca tgggcaatga agagataggt caggccaagc
     4381 cgctgctgca aatccatcag cagattgaca atctgggcct gcatcgagac atcaagcgct
     4441 gagaccggct cgtcgcagac aataaaatcc ggattgagcg cgagcgaccg ggcgatgacg
     4501 atgcgctgcc gttgcccgcc ggaaaactca tgcggatagc gatcggtact gatcttcggc
     4561 agtcccacca gctcgaacag ttcggcaacc cggtcgcttc ggtcccggcg cgacaatttt
     4621 ccggcaatct ccagcggctc cgcgaccgag gtgccgacgc gcatgcgggg attgagggca
     4681 gcatatggat cctgaaatac gaattgcaga cgcgaacgaa ccgcgccaag ggctgccgcc
     4741 ccgtcccggc cggtgatgcg gcgcccgaac aacgaaacgg tgcctgcggt cgccggaatg
     4801 agcccgacca gaatacgacc ggcggtggat ttacccgaac cggattctcc gaccagtccg
     4861 agcgtttcac cgggcatgac gtcgaaattg acatcatcca gcgcgttcag cggcgacgtc
     4921 ccgccggcgt ggccgaccat gaatgtcttt ttgaggccgc gcacctccac gatcggcgcg
     4981 tcagccattg acgtcaccaa gggcaagcac ctggggcggg cagcggacgg tatgcaactc
     5041 ccccgccggt tcgattggcg gcggcgtttg caggcagcta ttttgtacaa acccgcacct
     5101 gggcgcgaag cgacaccccg ggatattctc ctgcggcccg gggattcggc cgggaatggc
     5161 cgagaggcgc gtggcgtcgc catcgagttt cggcaccgcg gccatcagcc cgacggtgta
     5221 ggggtggacg ggtcgacgga aaagctgcgc gaccggcgcc tgctcgacga cgcgtcccgc
     5281 atacatgacg acgacgctct gggcgaactc cgcaatgaca ccgaggtcgt gcgagatcag
     5341 catcaccgac atgccgagtt ccttgcgcaa ctcgcgcaaa agctcgagga tctgggcctg
     5401 gatggtgacg tcgagcgcag ttgtcggctc atccgcaatc aaaagacgcg gccggcacgc
     5461 gatggccatc gctaccatga ccctctggcg catgccgccc gacagacgat gcgggtaatc
     5521 gtcggcgcgg cggcgggcat cgggaatgcg gacaagatcg agcaactcga ccgcgcggcg
     5581 ccgcgcggcc gtcccggaca ttttttggtg ttgccgaatt gtctcggaaa tctgttcgga
     5641 tatcgtcagc accggattga gcgacgacat cggatcctgg aagatcatcg cgatctcgcc
     5701 gccgcgaatg ccgcgcagcg aaagcttcga cgatttcagc agatcctgtc ctttgaatac
     5761 gattgaacca ctggggatgc gggttgtcgc acgatcatgc agccgcagga tggaaagcgc
     5821 ggtcacgctc ttgccgctgc cggattctcc gaccacgccg acacaatcgc cttccccaat
     5881 gtcgatcgac acgccctcga ccgccgtcac ccaaccggca tcgacgcgga actgcaccga
     5941 cagatcgcgg atcgcgagca gcggggcggc ggccactcta gtcgcctccc ttcaggcgcg
     6001 ggtccagaac atcgcgcagc gcgtcgccga gtagattcat gcccagcgcc gcaagactga
     6061 tggctatgcc cgggaacagc atcacccacc aggcttcggt ggcatagtcg cgtccctccg
     6121 cgatgatgtt gccccagctt gccaacggcg gctgcacgcc gacgccgaga aagctcagcg
     6181 ccgcctcggc aaggatggca taggcaaaga tgaaggtcag gtggaccagc agcggactga
     6241 aacagttcgg caaaatatga cgcacgatga tccgcagggc tgatgcgccc gacaatcgcg
     6301 ccgcttgcac atagtccatc tcgcgcacga ccagcgccgc cgcgcgaacg atgcgcacgc
     6361 cggccggaac ataggccacg gtcagggcgg caatcacgct ggagacgcgc gggccaagcg
     6421 ctgcggcaat gcccagcgcg agaagaatgg ccggaaacga catcaacgcg tccatcaacc
     6481 tcatgatggg cgcatcgagc ctgcggaatt gcgcggcgac gaccccaagg acggcgccac
     6541 aaaagccgga aataaccgag acggcgagcc caacgaacaa cgagacgcgg gcgccataaa
     6601 gcacgcgggt aaaaatgtcg cgcccgaaca tgtcggtacc aaagatgaaa tccgatgcgg
     6661 ggctgcgaaa gcggaaccga actttcatcg ccgaaggatc gagcgaggtc aaaagcggcg
     6721 caaagacagc cgcgagcgca agtacgacga tgatcacaaa gccgatcacg aaagacctgt
     6781 gcctgaacaa cctgcggatc aagcggccac gccgcggcgg agccaggcct gccgtgctca
     6841 cgatggcctc caagccgata tcagtcatgg cgcacccggg gatcggcgag tgtgtagagc
     6901 acatcgacga ccaggttgat cagtacaaag gtgaaaccga agatcagcat ggttccctgc
     6961 accagcgggt aatcgcgggc caggatcgcc tgcacaacca tccggccgat acccggcagc
     7021 gcaaacacct gttcgatgac aaccgagccg ccgatcgccg tgctcaataa tattcccgtc
     7081 gcggtaatga tgagaatcag agcattgcga agcgcgtgct tgccgaccgt gcgccactcg
     7141 ctgagcccct tggcgcgcgc cgttcgaatg aaatcctggc ccatgacatc gagcatcgcc
     7201 gagcgtgtca tgcgcgcgag aaagccgatc tgaaataatg acaggatcag cgccggcagg
     7261 atcacggacg agagccacgg cccgacccct tgcgagatcg gcgtgaagcc gctcgacggc
     7321 acccatccga ggcgaacgct gaacaggatg atgccgagaa tggcaatcca gaaggccggg
     7381 atcgacacgc caagcagcgc gaacgtcatg acgccggcgt cgatccatga ttgccgataa
     7441 taggccgcca gcgagcccag aaaaacgccc accggaatcg tgacggcgat cgacagcgct
     7501 gcgagcgaaa gggtaaccgg aaggcgctcg ccgaccgccg aaagcacgct ctggttcagg
     7561 atcagcgatg aaccaaaatt accctgcgcc agattaagca accagattgc cagttgttgc
     7621 aggaacgggc gatcgaggcc gagctgctga cgcacgtgtt cgacggcttc cggcgaagcc
     7681 tgctcgcccg cgatgatgat ggccgggtcg cccggcagca ggctgagcag gagaaaggcc
     7741 aggatcgaga tgatcagtat caccggcacg gcctgcagga acttgaaggc gatgatgcgt
     7801 agcagagcca actcccccga cggccggtgc tgatttttcg gatcctactc cagccagaga
     7861 tcccagagct ggagcacgcc ggcatagtcg ctttggccct tcagcttctt tgcgtaaccg
     7921 ctaagcctcc cggtatcggc gatcttgatg agattggcgc cgccaagaac ctgttgctga
     7981 atcttcagcc atgccgcccg ccgctggtcg agcgtgggcg ctgcaaaaaa ctcgccgtag
     8041 gccttatcga gcgcgtcgtt gccggtgatg tgaggaaatg tgtagagcat cggccgccac
     8101 tgttggggac ccagaacatg atctggcccg aaggccgtgg tcgacaggtt ccagtcgccg
     8161 gtgccttgct gcatatgcgc ggcgttggtg gtccaatcgg tgacctgaac gtcgacgttg
     8221 attccggcgg ccttgagctc ttcggcgaca
//
LOCUS       DATNOI010000691         5234 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_743012,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000691 DATNOI010000000
VERSION     DATNOI010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743012"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..355)
                     /locus_tag="VN968_27665"
     CDS             complement(17..355)
                     /locus_tag="VN968_27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXL33045.1"
                     /translation="MKKIEAIIKPFKLDEVKEALQEVGLQGITVTEAKGFGRQKGHAE
                     LYRGAEYIVDFLPKVKIEIVISDDLVERAIDAIRRAAQTGRIGDGKIFVSNIEEAIRI
                     RTGESGLDAI"
     gene            722..2221
                     /locus_tag="VN968_27670"
     CDS             722..2221
                     /locus_tag="VN968_27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HXL33046.1"
                     /translation="MEVLDTAEMERADRLTIAAGTPGFALMLSAGQAVAEAAIDLVEE
                     GSILVVAGGGNNGGDGFVAAAELAARGREVSVILLCDRDSLQGDAASAARGWKFPVLP
                     FNPQAIGRPALIIDALFGAGLNRAVKGDPLDMIEAINANGAPVLSVDLPSGVNGTTGA
                     VMGVAVRATETVTFFRRKPAHLLMPGRMHCGRVRVADIGIDADVLGEIHPQTFENVPQ
                     FWRKSFPVPQIDGHKYARGHAVVVSGDIAATGAARLSARGCLRAGAGLVTLASPTDAL
                     AVNAAALTAVMVCAIDTAVEFGELLTDKRLNTCVIGPGAGVGERTRDFVLTALSARRH
                     LVLDADALTSFAGAPDQLFQSIKASQDQQVVLTPHEGEFPRLFSDISNKHPYRSKLER
                     VRAAAERSGAIVLLKGPDTVVASADGRATIAANAPPWLATAGAGDVLAGMIAGLLAQG
                     VPAFEAASIGAWMHGEAAREAGPGLIAEDLPEVLPAVFRRLYDEFGIDY"
     gene            complement(2259..3341)
                     /locus_tag="VN968_27675"
     CDS             complement(2259..3341)
                     /locus_tag="VN968_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXL33047.1"
                     /translation="MRTTTATIRSVKARAVVAPISRPVKNAFGVIEAAPLVLIDVATD
                     RGVTGRSYIFAYAKLTLAPLVHLIEQIGGDLTGKAIAPFDLMADMDAKFRLLGWQGLV
                     GMAVSGLDMAFWDALGQVAGQPLAKLLGGSPRPIRAYDSYGVVDPIADEKDLRRSLDR
                     GFRGIKIKGGDGDIDNDERVVKAVRVLIGPDVALMLDFNQSLDPSEARRRIDRLAPYD
                     LHWIEEPVAAENLQGHATVRATSPISIQSGENWWFPRGFAEAIAAGASDFIMPDLMKV
                     GGVTGWLRVAGQAEANSIPMSSHLFAEASSHMLAVTPTAHWLEVLDLARAILAEPIEI
                     DDGTVTARGPGLGLSWDEAAVAKYLV"
     gene            complement(3351..4673)
                     /locus_tag="VN968_27680"
     CDS             complement(3351..4673)
                     /locus_tag="VN968_27680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HXL33048.1"
                     /translation="MADRIFPLNRRELMAGLGAAALGPAIPPMAVAQGRTSLTLQAKA
                     GAIALRPGQPETPIWSLGGPTTDRDVGLKRGDAVEITLLNDLPVPTVLNWHGLDGVPA
                     AEPLAAVTPLAPGAKQTFAIPLRHAGTLMCDLRLLGDGQARPAAARALIVGESEPVPV
                     DRDEVFLIEDWRLRPDGTAIAPSVDPKDTTPVTTINGLRTLDIPARANERLRFRFVNG
                     CQRSVIAIKIEGHEVRVMAIDGQPAEPFLARNGAVVLAPGTRVDVFIDVTAPPGSTSS
                     ILLHDGKEARPVARLIVSHEAAIRNAPLPPAPPLPSNGLPAQLDLKSALRIDLTLGGP
                     QADWVTPPNFTKSAAPAFRAKAGRTVVLALTNRAEVATVFHLHGHHFRLLDRLDDGWK
                     PFWLDRLAVEPGQTQRIALSAEYAGRWLIETATTDWAAPRLVRWYSVE"
BASE COUNT          909 a         1642 c         1721 g          962 t
ORIGIN      
        1 tatcggcggg accggttcag atggcatcca gtccggattc cccggttctg atgcgaatgg
       61 cttcctcgat gttggacacg aagatcttgc cgtcgccgat acgcccggtc tgggccgcgc
      121 gcctgatcgc gtcgattgcc ctttcaacca gatcgtccga gatcacgatc tcgatcttaa
      181 ccttgggtag aaagtctacg atgtattccg cgccgcgata gagttcggcg tgacccttct
      241 gccgcccaaa gcccttggct tcggtgacgg tgatgccttg cagtccgact tcctgaagcg
      301 cctctttcac ctcgtcgagc ttgaatggct tgatgatggc ctctattttt ttcactgaac
      361 gcctcccggg cacttccata tttcagactg caggtttgtt tccacgctgc gcgtggagcg
      421 aaggttgttc gaccgtctgt cctggcgggc agccggggac acgtcaataa atggctgttg
      481 cgcacgatca ctcagagccg gcttcctcaa aagcagggtc tatgccaagt tgttaaaacg
      541 cctgattttg aaatgttatc agacttttat cgggcgttcg cgtggcgtta ggcctagggg
      601 ctcacaatac tcgcgcccat gaaataggca aacggatgaa tccgtgtgca gtcgtcgaag
      661 gttggactta gcacgggacc ggaaatgcct gagtataagg cgcttggatc ggaaatttgg
      721 catggaagtt cttgacaccg ccgagatgga gcgcgccgac cggctgacca ttgccgcggg
      781 cacgcctggt ttcgcgctga tgctgagtgc gggtcaggcc gtggcggagg ccgccatcga
      841 cctcgtggag gaggggtcga tcctggtggt cgccggcggc ggcaacaacg gcggcgacgg
      901 cttcgtggcc gcagctgaac tggccgcgcg cggtcgcgag gtctcggtca tcctgctgtg
      961 cgatcgcgac agcttgcagg gcgacgcggc ctcggcggcg cggggatgga aatttccggt
     1021 gctgccgttc aatccgcagg cgatcggcag gccggcgctg atcatcgatg cgctgttcgg
     1081 tgccggtctg aaccgggcgg tgaagggcga tccgctcgat atgatcgaag cgatcaatgc
     1141 caacggcgcc ccggttctca gcgtggacct gccgagcggc gtcaacggca ccaccggcgc
     1201 cgtcatgggc gtcgcggtcc gcgccaccga aaccgtcacg ttcttccgcc gtaagcccgc
     1261 gcatttgtta atgccgggca gaatgcactg cggtcgcgta cgggttgccg acatcgggat
     1321 cgatgcggat gtgctcgggg aaattcatcc gcagaccttc gaaaacgtcc cacagttttg
     1381 gcgcaagtca ttcccggtgc cgcagatcga tggccataaa tacgcccgcg gccacgcggt
     1441 cgtcgtctcc ggcgacatcg ccgccaccgg tgcggcgcgg ctgtcggcgc gcgggtgctt
     1501 gcgggctggc gctggcctgg tcacgctggc ctcgccaaca gacgcgctcg ccgtcaacgc
     1561 ggctgcgctc accgccgtga tggtttgcgc catcgatacg gcggtcgagt tcggcgaact
     1621 gctcaccgac aagcgcctca atacctgcgt gatcgggccg ggcgccggcg tcggcgagcg
     1681 cacccgcgat tttgtcctga cggcgctatc tgccaggcgg cacctggtgc tggatgcgga
     1741 tgcgctgacg agtttcgccg gcgcccccga ccagctgttt cagtccatca aggcgtcgca
     1801 ggatcagcag gtggttctca cgccgcatga aggagaattt ccgcggctgt tcagcgatat
     1861 cagcaacaag catccatatc gctcgaaact cgaacgtgtc agggcagccg ccgagcgctc
     1921 tggcgccatc gtgctgctca agggaccgga caccgtggtg gcgtcagcgg acggacgcgc
     1981 caccatcgcc gccaacgcgc cgccctggct cgccaccgcg ggcgccggcg acgtgctggc
     2041 aggaatgata gcgggcctgc tggcgcaagg cgtgccggca ttcgaagccg ccagcatcgg
     2101 agcctggatg catggcgagg cggcgcgcga ggcgggtccc ggtctgatcg cggaagatct
     2161 gccggaggtg ctgcccgcgg tttttcggcg cctctacgat gagttcggca tcgactattg
     2221 agacgtcctg gaagcatcca tggtttgtca caagccgctc acaccagata tttcgccacc
     2281 gccgcctcgt cccaggagag gccaaggccg ggcccgcgtg cggtgaccgt gccgtcgtcg
     2341 atctcgatcg gctcggccag gatggcgcgg gcaaggtcga gaacttcgag ccagtgtgcg
     2401 gtaggcgtga ccgccagcat gtgagagctg gcttcggcga acaggtgact ggacatcggg
     2461 attgaattag cctctgcctg gcccgcgacg cggagccagc cggtgacgcc gccgaccttc
     2521 atcagatcag gcatgatgaa gtcgcttgca cctgccgcga tcgcttcggc aaagccgcgg
     2581 ggaaaccacc agttctcgcc ggactgtatc gatatcggcg acgtcgcgcg caccgtcgca
     2641 tggccctgaa ggttttcggc ggccaccggc tcctcgatcc aatgcagatc gtagggtgcg
     2701 agccggtcaa tgcggcggcg ggcctcggac ggatcgagcg actggttgaa atccagcatc
     2761 agcgcgacgt cggggccgat caggacgcgc accgccttca cgacccgctc gtcgttgtcg
     2821 atatcgccgt cgccgccctt gatcttgatg ccgcgaaagc cgcgatcgag cgaacggcgc
     2881 aggtccttct cgtcggcgat ggggtcgacc acgccataac tgtcataggc ccggatcggc
     2941 cgcggcgatc cgcccagcaa cttggccaac ggctgaccgg ctacctggcc cagcgcgtcc
     3001 cagaacgcca tgtcgaggcc cgacacggcc atgccgacca ggccttgcca gccgagcaat
     3061 ctgaatttgg cgtccatgtc agccatgagg tcaaacggcg cgatcgcttt gccggtaaga
     3121 tcgccgccga tctgttcgat taaatgcacc agcggcgcca gcgtcagctt ggcataagca
     3181 aaaatgtagg agcgcccggt aacgccccga tcggtcgcga cgtcgatcag caccagtggc
     3241 gctgcctcga tgacgccgaa ggcgttcttc accggccgcg agatcggtgc gacgacggcc
     3301 cgggccttga cgctgcggat ggtcgcggtg gttgtgcgca ttctcgttcc tcactcgaca
     3361 ctgtaccacc gcacgaggcg gggcgccgcc cagtccgtcg tggcggtctc gatcagccag
     3421 cgccctgcat attccgctga cagcgcgatc cgctgggtct gaccgggttc gaccgccaac
     3481 ctatccagcc agaacggctt ccagccgtcg tcgagccggt cgagcagcct gaaatgatgt
     3541 ccatgcaggt ggaacacggt ggcgacctcg gcgcggttgg tcagcgccag caccacggtg
     3601 cggcccgcct tggcgcggaa ggcgggcgca gcggattttg tgaaattcgg gggcgtgacc
     3661 cagtcggctt gtggtccgcc gagcgtcaaa tcgatgcgca acgcgctctt gaggtcgagc
     3721 tgggccggca ggccgttgga gggcagcgga ggggcgggcg gtagcggggc gttccggatc
     3781 gctgcctcat gggagacgat cagccgcgcg acgggacggg cttccttgcc atcatgcaga
     3841 agaatcgatg aggtcgagcc cggtggcgcc gtcacgtcga tgaacacgtc gacccgggtt
     3901 cccggcgcca gcacaactgc gccgttgcgc gccaggaacg gttcggccgg ctggccgtcg
     3961 atcgccatga cacggacctc gtgtccctcg atcttgatag caatgaccga ccgttggcag
     4021 ccatttacga agcggaacct cagccgttca ttggcacgcg ccgggatatc cagcgtcctc
     4081 aacccattga tggtggtgac cggcgtcgtg tccttcggat cgacgctagg tgcgatcgcg
     4141 gtcccgtccg ggcgcagccg ccagtcttcg atcaggaata cctcgtcgcg atcgacgggg
     4201 acgggctcgc tctccccgac gatcagcgcc cgcgccgccg ccggccgcgc ctggccatcg
     4261 cccagcagcc gaaggtcgca catcaacgta ccggcgtgac gcaatggaat cgcaaaggtt
     4321 tgcttggcgc ccggagccag aggcgtaaca gccgccagcg gttctgccgc cgggacgcca
     4381 tctagcccgt gccagttgag taccgtgggc actggcaagt cgttcagcag ggtgatttca
     4441 accgcgtcgc cgcgtttcaa gccgacatcg cggtctgttg ttggtccgcc gagcgaccat
     4501 atcggcgtct ccggctgtcc cgggcgcaag gcgatggcgc cggccttggc ctgaagcgtc
     4561 agggacgtgc gcccctgagc gaccgccatt ggcggtatcg caggaccgag cgccgcagcg
     4621 cctaatccgg ccatcaactc gcgtcggttc agcgggaaaa tccgatctgc catgagatcg
     4681 atgcggacca tttttcgcag gatatgtcca gcgcgtagga taggtggagc gaagggctca
     4741 tcgtagcccg gatggagcca acgggtcgcg cgaatgcgcg cccgatgaca ggctccgcga
     4801 aatccgggac tcacgcgacg tcggccccgg attgcgcttg gctccatccg ggctacagcc
     4861 gcgtgatttg cccgtcgggc tgtttgtcga caggcgtgtc gagtcttatt tctgattttc
     4921 cgaaaaatat tttcgttccc acgcacccca aatcagatct agaactttac gcatcccacc
     4981 ccaccagagg ggcgtatcat gatcgtcacg gacgcgggga gggatgcggt ggacgcggca
     5041 gcgttaggcg cgcgacggga ttgcagggcg agttgaaaga cttgtgagcg atcaacagcg
     5101 agcggacgag agatgttgca gcgtacggcg aagtcgtgtg gtcctgacgc cccgatgctg
     5161 gcgtcaagct cgcggagttt aagtcggccc gaccgggctt agacaaaaac atatccgttg
     5221 gcgacggtgg caaa
//
LOCUS       DATNOI010000692         2233 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1642388,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000692 DATNOI010000000
VERSION     DATNOI010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642388"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VN968_27685"
     CDS             complement(<1..1059)
                     /locus_tag="VN968_27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL33049.1"
                     /translation="MKILIFGGTGFVGLNIAAALLVRGHAVTLFDRANLPAAAQHDFA
                     GYREALTIVHGDITDRRAVMDVVAGGHDAIVLGAAITAGPAREAADPEQIMQVNLLAQ
                     LPVLVAARRSGVARIINLSSGAAYGAAGARHALLDEETACDPESVYAITKFASERVAA
                     RLVALWHFDIVSVRLSSVFGRWERATGVRDTLSPHAQILATLRQQGEAVLPRPGVKDW
                     IYSPDVADAVTLLIEAPKPKHHLYNISTGREWPALRWGQDLAKLHPGFVCRLAEPGET
                     PTIDLHGPTDRAPLSVSRLEQEFGWRARFGCAESGGCSIRKGHNMRLKGQIAIVTGAG
                     SGIGRATALLFANEGAFVA"
     gene            complement(1139..>2233)
                     /gene="hydA"
                     /locus_tag="VN968_27690"
     CDS             complement(1139..>2233)
                     /gene="hydA"
                     /locus_tag="VN968_27690"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HXL33050.1"
                     /translation="AFHMIIADPTPETLQNDIPELVKQGHGSIKIFMTYDLLKIDDEP
                     LLDILVAARESGAMLCAHAENHGIIAWMVKRLLARGYTHPKYHAIGHARFSEADAFNR
                     LIGMAALVDQPIMIFHVSTADGAKVIRDARGQGLKVFAETCPQYLFLTADDLDKPGVD
                     GAKWMCSPPPRRVADQDALWQALALGDLQTVSSDHAPYRFDETGKLRAGANPNFKQIA
                     NGMPGLQVRLPLLFDAMVSKGRLGLEKFVELTATAPAKIYNLHPRKGSIAVGADADIA
                     IWDPEREVMLTDAMMHDLAGYTPYAGRQLRGWPVTVLSRGRVIVADGKRVAEPGSGRF
                     LARSGGDAAKPTGRLVADMDPARNFGATLL"
BASE COUNT          422 a          735 c          695 g          381 t
ORIGIN      
        1 cgcgacaaac gcgccttcat tcgcgaacaa caatgccgtg gcgcgaccga tgccggaacc
       61 ggctccggtg acgatcgcga tctgcccctt cagtcgcatg ttatggccct tcctgatgct
      121 gcatccaccc gactcggcac agccaaaacg ggcgcgccag ccaaactcct gctccagacg
      181 cgaaaccgat agcggcgccc ggtccgtcgg gccgtgaaga tcgatggtcg gcgtctcgcc
      241 gggttcggcg agacggcaga cgaaaccggg atgcagtttg gccagatcct gtccccatcg
      301 cagcgccggc cattcgcgcc cagtcgaaat gttgtagaga tgatgtttcg gcttcggcgc
      361 ttcgatcagc aacgtcacgg cgtccgcgac atccggggag tagatccagt ccttgacgcc
      421 cggacgcggc agaacagctt cgccttgctg gcgaagggtg gcaaggatct gcgcgtgtgg
      481 actgagcgtg tcgcgcaccc cggtcgccct ttcccatctc ccgaacacgc tgcttaaccg
      541 cacactgacg atatcgaagt gccagagcgc aaccagacgc gcggcaactc tctcggaagc
      601 aaatttggtg atggcataga ccgactcagg atcgcaggcc gtctcttcat cgagcagggc
      661 gtgccgcgcg ccggcagcgc cataggccgc gccggatgac agattgatga tccgcgcgac
      721 gccgctgcgc cgcgccgcga ccagaaccgg caactgcgcc agcagattga cctgcatgat
      781 ctgttcaggg tccgccgctt cgcgcgcggg gccggcggtg atcgctgccc cgagcacgat
      841 cgcatcatgg ccgcctgcga caacgtccat gacagctcgc cgatcggtga tgtcgccgtg
      901 aacgatagtc agcgcttccc ggtagccggc gaaatcgtgt tgcgcggcag cgggcaggtt
      961 cgcgcgatcg aacagggtga cggcgtgccc gcgcaccagc agggcggcgg cgatattgag
     1021 cccgacaaaa cccgtaccgc cgaagatcag gattttcatg ccccggcccc agcacctgtc
     1081 gcgatcagcc gcgacgacca tcgccatcga tgcatccggc gacagtacga caaaatgctc
     1141 acagcagggt cgcaccgaaa ttccgcgccg gatccatatc ggcgacaagt cggcctgtcg
     1201 gcttcgcggc atccccgccg gaacgcgcga ggaagcgccc ggaaccaggc tcagccacac
     1261 gcttgccgtc cgcgacgatc acgcgtccgc gcgacagcac cgtgaccggc cagccgcgta
     1321 gctggcggcc ggcatagggc gtgtagccgg caaggtcgtg catcatggcg tcggtcagca
     1381 tgacctcgcg ctccgggtcc cagatcgcga tatcggcatc agcgccgacc gcgatagagc
     1441 ccttgcgcgg atgcaaattg tagatcttgg caggcgcggt cgctgtcaac tcgacaaatt
     1501 tttccaggcc caggcggccc ttcgacacca tcgcgtcgaa cagcagcggc aggcgcacct
     1561 gcagtcccgg cataccgttc gcgatctgct tgaagttggg attggcgccc gcgcgcaatt
     1621 tgccggtctc gtcgaaacga tacggcgcat ggtccgacga tacggtctgc agatcgccga
     1681 gcgcaagcgc ctgccacagc gcgtcctgat cggcgacgcg gcgcggcggc gggctgcaca
     1741 tccatttggc gccgtctacg cccggcttgt ccagatcgtc ggcggtgaga aatagatatt
     1801 gcgggcaggt ctcggcgaac actttcaggc cctgcccgcg cgcgtcacgg atcaccttgg
     1861 cgccgtccgc ggtcgacacg tgaaagatca tgatcggttg atcgacgagc gccgccatgc
     1921 cgatcagccg gttgaaagcg tcagcttccg aaaacctcgc atggccgatg gcgtggtatt
     1981 tcgggtgggt gtaaccgcgc gccagcagcc gcttcaccat ccatgcgatg atgccatggt
     2041 tctcggcatg ggcacacagc atggcgccgg attcacgtgc cgcgaccaga atgtccagca
     2101 aaggctcgtc atcgatcttc agcaagtcat atgtcatgaa gatcttgatc gagccgtggc
     2161 cctgtttgac caactccgga atgtcgttct gcagggtctc tggagtcgga tcggcgatga
     2221 tcatgtgaaa cgc
//
LOCUS       DATNOI010000693         7546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_797805,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000693 DATNOI010000000
VERSION     DATNOI010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797805"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VN968_27695"
     CDS             complement(<1..252)
                     /locus_tag="VN968_27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HXL33051.1"
                     /translation="MLTRRGILLASIAAGMTTMPNRSVFAAAAQPSTPVNFDIPAGAC
                     DCHTHIHGDPAKFPFFAGRVYTPELASPEEMSALHKALHI"
     gene            complement(313..1026)
                     /locus_tag="VN968_27700"
     CDS             complement(313..1026)
                     /locus_tag="VN968_27700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXL33052.1"
                     /translation="MLQACLNGNRDKVFHAATPLTPRELAADARAVVEAGAEQLHIHP
                     RDAEGRESLHPDDTAAALEAIRASVPGIPVGVSTGWWIAPGGRARQQHIRAWQVKPDY
                     VSVNLIEQDAGEVIDLVLEKGIGVEAGLWSAADAGKFVAHPNARNCLRVLIEINEQEF
                     LAGAEAVRQIVEILAHAEIQLPRLLHGFERTMWPFYREALRLKLDARIGLEDGKLLPS
                     GAEAENNAALIRAACALTG"
     gene            complement(1049..2995)
                     /locus_tag="VN968_27705"
     CDS             complement(1049..2995)
                     /locus_tag="VN968_27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HXL33053.1"
                     /translation="MRQLRSAIALIAGIIGLTGPSTAKVIKFEIVYVESPTFEGRSFG
                     AVGTYDRIVARATVAVSPDDPHNTIIVDLDRAPRNAQGWIEAVTDVEILRPTDAANGN
                     RRLFYEALNRGNKLSLALFNDSPVPVNDLAKTADAGNGFLMSSGYTIVWSGWQGDVAP
                     GGGRMTFSPPVVPDITGLAREDFIFDHMDNPASATLNYPTADLDPAHAKLSVRQREAD
                     PRATPAGLEARFEGPTRISIQRPQGFDAGAIYEFIYTAKDPKVMGLGFAATRDIVSFL
                     RNETADAVGTANPLAGRIDRAIGFGASQSGRYLQDYLYLGFNADEAGRTVFEGLMPHI
                     SGGKKTFTNYRFSQPGRSPYQHADMLYPGADFPFTYPVITDTLTGKSDGFLARCLAAG
                     NCPKIIKTDSEIEFYQQRASLVVTDTEGNALAMPDNVRLFLLSNLQHFALAGTRSEMT
                     KTCAFPTNPLNAGPPARALLVALDDWISNGTLPPASRYPSRGDGTLVPPTIEDVGFPH
                     IPGFAYTSRMARPTLIKSDEMPPSKGAAYPVFVPKTDADGRDIAGLHLPTLGAPSATH
                     TGWNLRKAGFGEGELCDNNGAMIPFAATREERLKNNDPRLSMAERYPNEGDHAAAIAK
                     AARQLVQDRLLLEDDAKLFATNVN"
     gene            3185..4783
                     /locus_tag="VN968_27710"
     CDS             3185..4783
                     /locus_tag="VN968_27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXL33054.1"
                     /translation="MRKFSKKFKTSASAIALLVVVFGVYGLRKLQVLAADPIGEKDCR
                     PAADGAEQGRSDLDRIKAIAPLADVKWSQLGGSINDASCLNKTDIYGVVEVRSVDDVA
                     KTLAFARDNKLSITTAGVRHSMGGQAFRKGGIVLDMRGFNRIVLNESTRTITVQPGAT
                     WHDIQNVLHPRFAVRAMQSTDIFSVGGSISVNAHGMDHQAGAVAKSIKSMRVMLADGS
                     LRTVSPTENRELFDLVVGGYGLFGVIVEAELDIADNLVYQTGRHVLDYRVFPALFAGE
                     IEKDTNIGLMYGHLSTAPSTFLQEMLLYTYTKVDGEDFSRAPLGEVSGTKLRRLTINL
                     SKQGPLFQELKWLSEKHIEHRMETCTVTRAQALPSAEACLVSRNDPMHDSVPYLRDSL
                     PNDTDILHEYFIPRSQFVSFVDGMRKVLTDNKTNLLNASVRIVHQEENFLTYSPEPAF
                     SLVLYINQTTDDEGNRRMKKATEELIDLTIAHKGRFFLPYQLYYSRDQLQRSYPQIND
                     FFAAKRRYDPAELFTNTFYQKYAK"
     gene            complement(4796..5146)
                     /locus_tag="VN968_27715"
     CDS             complement(4796..5146)
                     /locus_tag="VN968_27715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33055.1"
                     /translation="MIIEGRRAVSPWPRPLGRIAAATALVAAGWFAIVVALTFASAPG
                     KSMAIVGPPARALAAVVKANGHILTSSSYVTIARSDDAGFVARLYAAGALLVLDAEQA
                     GGCGGLPPKRAASL"
     gene            complement(5136..6236)
                     /locus_tag="VN968_27720"
     CDS             complement(5136..6236)
                     /locus_tag="VN968_27720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33056.1"
                     /translation="MVKCLRRILASIATLIALSVIVSLTYIEGSCRPAAAVAASSAPG
                     QSLPAINEQGYRRKEANTFFTFPEWYIVYSFEDFGHFLDSSSESQFGYLSHILGFWRS
                     FCTINRAVPATDESLGDVKSMIYVIGVSYSAEYAIKGFYENTIGRITEWIRGSVRTPQ
                     DDYARTVLQDYAAFLYTIPWYKYPFGEKLHGLMAISAPTPSHIRTWERDFALGAEYFI
                     KIGYAWLIQKALDASSDDEPRDIMLVVATLPPATLAKEPRIKQVRALTPEWQLVQTPR
                     YKDLTEIVQSLLDQGLPIAEIAGNHEILITVIAPKSATLEVNAATELFSLDLDAKPGF
                     RRAGLRARIDRLVDINRALKARGASIEHFYDY"
     gene            complement(6229..6651)
                     /locus_tag="VN968_27725"
     CDS             complement(6229..6651)
                     /locus_tag="VN968_27725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33057.1"
                     /translation="MDIIRLCAWGYAALLSFVIMTGYVPAFIDQNDMIFGLFRRTWYA
                     DGLHLVSALWAATAALTSRRASELFFQLFGVFYFADGVLGLLTGSGYLDFGILINGVR
                     DLPLMTRIFTNAPHLALGGMAILIGYVLAPRTRAIVHG"
     gene            complement(6853..>7546)
                     /locus_tag="VN968_27730"
     CDS             complement(6853..>7546)
                     /locus_tag="VN968_27730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXL33058.1"
                     /translation="DRYLPHSREEAIDLALKGHPAVIAASFDVDVASTTIHVAESSLL
                     PTITVQGNASHSSDADPSLTTFRTDQASILGQMTAPIYDGGTAASQTRQAKELAAQSR
                     QVLDQVRNQSRTAATGAWVANEGAKVAVTASESEVRAAGVALQGVQKEAAGGQRTTVD
                     VLNSQQDLISAKARLIGAQRDRVIASYTLLSAIGKLDVKTLALNTPDYLPEVHYHQVR
                     DAWHGLRTPSGK"
BASE COUNT         1409 a         2265 c         2424 g         1448 t
ORIGIN      
        1 gatatgcagc gccttgtgca gggccgacat ctcttcgggc gacgccagtt ccggggtata
       61 gacgcggcct gcgaagaacg ggaatttggc ggggtcgccg tggatgtggg tgtggcagtc
      121 gcacgcgccg gcgggaatgt cgaaattcac cggcgtcgag ggttgcgccg cggctgcaaa
      181 aacggatcgg ttgggcatgg ttgtcattcc cgctgcgatg gaggcaagca agataccgcg
      241 tcgcgtcagc atggtcgtct ccctgttgcg ttttccgcgc ttttggtttt gcgcttggtg
      301 caaagtagca ggttatcccg tcaacgcaca cgccgcgcgg atcaaggcgg cattattttc
      361 ggcttcggct ccggacggca gcagcttgcc gtcttccagt ccgatccgcg catcgagttt
      421 gaggcgaagc gcttcgcgat agaacggcca cattgtgcgt tcgaatccgt gcagcaaacg
      481 cggtagttga atctcggcgt gcgccaggat ttccacgatc tgacgcacag cctccgcgcc
      541 cgccaaaaat tcctgctcgt tgatttcgat cagtacccga aggcagttgc gggcgttagg
      601 atgcgccacg aattttcccg cgtcggcggc agaccacagg cccgcctcga cgccgatgcc
      661 tttttcgagg acaagatcga tgacttcgcc ggcgtcttgc tcgatcagat tgacggagac
      721 atagtcgggc ttcacctgcc acgccctgat atgctgttgc cgcgcccgtc ctcccggagc
      781 aatccaccag ccggtcgata cgcccacggg aattccgggc acgctggccc tgatggcctc
      841 aagtgccgcg gcggtgtcgt cgggatgcag gctttcccgc ccctcggcat cgcgcggatg
      901 gatgtgcaac tgctctgcgc cggcctccac gacagccctg gcatcggctg ccagttcccg
      961 cggcgtcagc ggggttgcgg cgtggaagac cttgtcgcgg ttaccgttca ggcaggcttg
     1021 aagcatcggc aagactcacg gcaggacgct agttgacgtt tgtcgcgaac agcttggcgt
     1081 cgtcttccag cagcagccgg tcctgcacca gttgccgcgc ggctttggca atcgccgcag
     1141 cgtggtcgcc ctcgttcgga tagcgctccg ccatcgacag cctcggatcg ttgttcttca
     1201 gccgctcctc gcgggtcgcc gcgaatggaa tcatggcgcc gttattgtca cacaattcgc
     1261 cttcgccaaa accggccttg cgcagattcc agccggtgtg ggttgcgctc ggggctccta
     1321 gcgtcggcag gtgcagcccg gcaatgtcgc ggccgtcggc atcggtcttc ggcacgaata
     1381 ccggataggc cgcgcctttc gatggcggca tctcgtcgga cttgatcaaa gtgggccgcg
     1441 ccatgcggct cgtataggcg aagccaggaa tgtgcgggaa accgacgtct tcgatggtgg
     1501 gtggcaccag cgttccatcg ccgcggctcg gatatcggct ggccggcggc agtgtgccgt
     1561 tgctgatcca atcatcgagc gccaccagca aggcccgcgc cggcggaccg gcattgagtg
     1621 gattggtcgg gaaggcgcat gtcttcgtca tctcggatct ggtgcctgcc aacgcgaaat
     1681 gctgcaggtt cgaaagcagg aacagcctga cattatcggg catcgcaagc gcgttgcctt
     1741 ccgtgtccgt caccaccagc gacgcgcgct gctgatagaa ctcgatttcg ctgtcggtct
     1801 tgatgatctt cgggcaattg ccggcggcca ggcagcgcgc cagaaagccg tcgctctttc
     1861 cagtcagcgt gtccgtgata accggatagg tgaagggaaa gtccgcgccg ggataaagca
     1921 tgtccgcatg ctggtaggga gaacggccgg gctggctgaa acgataattg gtgaaggttt
     1981 tcttgccgcc ggaaatgtgc ggcatcagcc cttcgaacac cgtgcggccg gcctcgtcgg
     2041 cattgaagcc gagataaaga tagtcctgca gataacggcc gctttgtgag gcgccgaaac
     2101 cgatggcgcg gtcgatccgg ccagcgaggg gattggcggt ccccaccgcg tccgctgttt
     2161 cgttgcggag aaacgaaacg atgtcgcggg tggcggcgaa gccgaggccc atcaccttgg
     2221 ggtctttggc ggtgtagatg aattcgtaga tcgcgccggc gtcgaaacct tgcgggcgct
     2281 ggatcgagat tctggtcggt ccctcgaatc tggcttcgag gcccgcgggc gtggcgcgcg
     2341 gatcggcctc gcgttggcgc acgctgagct ttgcatgggc ggggtcgagg tcggcggtgg
     2401 gataattcag cgtcgcgctg gcgggattat ccatgtggtc gaagatgaaa tcctcgcggg
     2461 cgaggccggt tatatcaggc actacgggcg gcgagaacgt catccgcccg ccgcccggcg
     2521 cgacgtcgcc ctgccagccg ctccatacga tcgtgtagcc gctactcatc aggaagccat
     2581 tgccggcgtc ggcagttttc gcgagatcgt tgacggggac cgggctgtcg ttgaacaggg
     2641 cgaggctgag cttgttgccg cgatttagcg cctcatagaa caggcggcgg ttgccgttgg
     2701 cagcatccgt gggacgcaag atctcgacat cggtcacggc ctcgatccag ccctgcgcat
     2761 tgcgcggcgc gcggtcgaga tcgacgatga ttgtattgtg cggatcatcg ggcgacaccg
     2821 cgacggtggc gcgcgcgacg atccggtcgt aggtgccgac cgcgccgaag ctacgcccct
     2881 cgaacgtcgg cgattccacg tatacgatct cgaatttgat gacttttgct gtggaagggc
     2941 cggtcaaacc gatgatgccg gcgatcaaag ctattgcgct ccgcaattgc cgcatggcct
     3001 tctccccgat gtctgcgccg gcgttggccg tcggtcgcgt atccgagact acagggggaa
     3061 gtgtcagccg ggaagggcca tgcgattcgg aaaattcacc ggtttactgg tcgaaccgga
     3121 aaaccgccgg gtttgactaa tgctccacag gtctggatga tctgacgcaa gccggaatca
     3181 attgatgcga aaattttcga agaaattcaa aaccagcgcg tcggcgatcg ctctgctggt
     3241 cgtagtgttt ggcgtttatg gcttgcgcaa gcttcaggtc ctggccgccg atcccatcgg
     3301 cgaaaaggat tgccggcccg ccgctgacgg cgccgagcag ggcagaagcg atctcgatcg
     3361 gatcaaggcg attgcgccgc tcgccgatgt gaaatggtcc cagctcggcg gcagcatcaa
     3421 tgatgcaagc tgcctcaaca agaccgatat atacggcgtc gtcgaggtgc gcagcgttga
     3481 tgacgtggcg aagacgctgg cgttcgcccg cgacaacaag ctttcgatta cgacggccgg
     3541 cgtacgccac agcatgggag gccaggcttt ccgcaagggc ggcatcgtgc tggacatgcg
     3601 gggcttcaac cggatcgtcc tcaatgagag cacacgaacg atcaccgtgc agccgggggc
     3661 gacctggcac gatattcaga acgtgctgca tccgcgcttc gcggttcgcg ccatgcaatc
     3721 gaccgatatc ttcagcgtcg gcggttcgat ttcggtcaac gcccacggca tggatcacca
     3781 ggccggtgcg gtcgccaagt cgatcaaatc gatgcgcgtc atgctggcgg atggttcgct
     3841 gcgcacggtt tcgccgaccg aaaacaggga attgttcgat ctggtggtcg gcggctacgg
     3901 cctgttcggc gtgatcgtcg aggccgagct cgatatcgcg gacaatctgg tctatcagac
     3961 cgggcggcac gtgctcgact acagggtatt tccggcgctg ttcgcgggcg agatcgagaa
     4021 ggataccaat atcggcctga tgtatggcca cctctcgacc gcgccgagca cctttctgca
     4081 ggaaatgctg ctctatacct acaccaaggt cgatggcgag gatttctcac gcgcaccgct
     4141 cggcgaggtc agcggaacaa aactgcggcg gctgaccatc aatctgtcga agcagggccc
     4201 gttgtttcag gaattgaaat ggctgtccga gaagcacatc gagcaccgga tggaaacctg
     4261 cacggtgacg cgcgcgcagg cgcttccgtc ggccgaggcg tgcctcgtca gccgcaacga
     4321 tccgatgcac gattccgtgc catacttgcg cgactcgcta cctaacgaca ccgacatcct
     4381 gcacgaatac ttcattccgc gcagccagtt cgtctccttt gtcgatggca tgcgcaaggt
     4441 gctgaccgac aacaagacca acctgttgaa cgcgtcggtg aggatcgtgc atcaggagga
     4501 gaatttcctt acctattcgc cggagccagc gttttcgctg gtgctttaca tcaaccagac
     4561 caccgacgac gagggcaatc ggcgcatgaa gaaggcgacg gaagagctga tcgatctcac
     4621 gatcgcgcac aagggccggt tcttcctgcc ttaccagctc tattattcgc gcgatcagtt
     4681 gcagcgctcc tacccgcaaa tcaacgactt cttcgcggcg aaacgcagat acgatccggc
     4741 cgagcttttc accaatacgt tttatcaaaa atacgccaag tagaaccgcc acccatcaga
     4801 ggcttgcggc acgtttaggc ggcaagccgc cgcagccgcc ggcctgttcg gcatccagca
     4861 ccagcagcgc gcccgccgcg tagagccggg ccacgaaacc ggcgtcgtcg gaacgcgcga
     4921 tcgtgacata gctgcttgac gtgaggatgt ggccgttcgc cttgacgacc gccgccaggg
     4981 ctcgcgccgg cggcccgacg atcgccatcg acttgcccgg cgccgatgcg aaggtcaggg
     5041 cgaccacgat cgcgaaccag ccggcggcga ccaaagcggt tgctgcggcg atccggccca
     5101 atggccgcgg ccagggcgaa acggcccgtc tgccttcaat aatcatagaa gtgctcgatg
     5161 ctcgcgcccc tggccttgag tgcccgattg atatcgacaa ggcgatcaat cctagcccgc
     5221 aagccggccc ggcggaagcc gggtttggcg tcgagatcca gtgaaaacag ctcggtcgcg
     5281 gcattcacct ccagcgtcgc cgatttcggc gcgatcacgg tgatgagaat ttcgtgattg
     5341 ccggcgatct cggcaatggg gagaccctga tccagcaggc tttgaacgat ttcggtcaag
     5401 tccttgtagc gcggcgtctg caccagttgc cactccggcg tcagcgcgcg gacctgtttg
     5461 atgcgcggct ctttggccag tgtcgcgggg ggcagcgttg ctaccaccaa catgatgtca
     5521 cgcggctcgt cgtcgctgga ggcgtcaagg gccttctgga tcagccaggc atagccgatc
     5581 ttgatgaagt attccgcgcc gagcgcgaag tcgcgctccc aggtgcggat gtggctcggc
     5641 gtcggcgccg agatcgccat caggccgtgc agcttctcgc cgaatggata tttgtaccag
     5701 gggatggtgt agagaaacgc ggcatagtcc tgcagcaccg tgcgcgcata gtcgtcctgc
     5761 ggggtgcgca ccgagcctct gatccattcg gtgatgcgtc cgatggtgtt ttcgtaaaat
     5821 cccttgatcg cgtattcggc ggaatagctg acgccgatca catagatcat cgacttgacg
     5881 tcacctaagg attcgtcggt cgccggcacg gcgcggttga tggtgcagaa gctgcgccag
     5941 aatcccagaa tatgcgagag gtagccaaac tggctttcgc tcgaggaatc gagaaagtgg
     6001 ccgaaatcct cgaacgaata gacgatgtac cattcgggaa aggtgaagaa ggtgttggct
     6061 tccttgcgcc gatagccctg ctcgttgatc gcgggcaagg attgacccgg cgccgaggat
     6121 gccgcgacgg cggcggccgg gcggcaactc ccctcgatgt aagtcaacga cacgatgacg
     6181 gaaagtgcga tcagggtcgc gatggacgcc aggatgcgcc gcaggcactt aaccatggac
     6241 gatcgcccgc gtccgtggcg ccagcacata gccgatcagg atcgccatgc cgccgagcgc
     6301 cagatgcggc gcgttggtga aaatccgcgt catcagcggc aggtcacgga cgccattgat
     6361 cagaatgccg aagtcgagat agccgctgcc ggtgagcagg ccgagcacgc catcggcaaa
     6421 gtagaacacg ccgaacagtt ggaagaacag ttccgaggcg cggcgcgagg tcagcgccgc
     6481 cgtcgccgcc cacagcgccg agaccagatg aagcccgtcg gcataccagg tacgccggaa
     6541 caggccgaag atcatgtcgt tctgatcgat aaaggcaggg acatatcccg tcatgatcac
     6601 aaagctgagc agagccgcgt agccccaggc gcacaatctg atgatatcca tgatctctcc
     6661 agcctgccga atctgttgcg agttaagatt tcccgtgcgc catgcgaact tccgcgacca
     6721 gttcgggcaa cagccgatcc atcgatacac agaaccgggc agggcagagc ggcaatccac
     6781 gcctgatgcc ttgtcagact gcggccttcg cggatgtgaa tgcggcaact cttcatggcg
     6841 cgggtttgaa acctactttc ccgacggcgt gcgcaggccg tgccaggcgt cgcgcacctg
     6901 atggtagtgc acctccggca gataatccgg cgtgttgagc gccagcgtct tgacgtcgag
     6961 ctttccgatc gcgctgagca aggtgtagga cgcgatcacg cgatcgcgtt gggcgccgat
     7021 cagccgcgcc ttcgccgaaa tcagatcctg ctgcgagttc agcacatcga ccgtggtgcg
     7081 ctggccgccc gccgcttcct tttgcacacc ctgcagcgcc acaccggcgg cgcggacttc
     7141 ggattcggaa gccgtcaccg cgaccttggc gccttcattg gcgacccacg cgccggtggc
     7201 cgcggtgcgc gactggttgc gaacctgatc gagtacctgc cggctttgcg cggccagttc
     7261 cttggcctgc cgggtctgcg acgccgcggt gccgccatcg tagatcggcg ccgtcatctg
     7321 gccgaggatc gaggcctgat cggttcggaa ggtggtgagg ctcggatcgg cgtcgctgct
     7381 gtggctggca ttgccttgca cggtaatggt gggcagcagg ctgctttcgg caacatggat
     7441 ggtggtcgag gcgacgtcga cgtcgaagct ggcggcgatc acggcgggat ggccctttaa
     7501 ggcgaggtcg atggcttcct cgcggctgtg cggcagatag cggtcg
//
LOCUS       DATNOI010000694         2313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_633213,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000694 DATNOI010000000
VERSION     DATNOI010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633213"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VN968_27735"
     CDS             complement(<1..186)
                     /locus_tag="VN968_27735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33059.1"
                     /translation="MEAIVNPPSRGSRLDADHPKTGALFHADLQKMSSTIVEADPTTR
                     RVEASEQSFQTGFTDDIV"
     gene            212..1711
                     /gene="istA"
                     /locus_tag="VN968_27740"
     CDS             212..1711
                     /gene="istA"
                     /locus_tag="VN968_27740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HXL33060.1"
                     /translation="MLIVETIAKVRRAYFIQKKPIKAICREFRLSRRVVRKVIRSEST
                     EFRYERGRQPLPRIDPWRDQLDGLLLANEEKPARERLTLIRVFEELSGLGYEGGYDAV
                     RRYARVWRAARGAATAQAYVPLSFAPGEAYQFDWSHEIVVMNGVTTTVKVAHVRLCHG
                     RMPFVRAYPRESQEMVFDAHARAFDFFKGACTRGIYDNMKTAVETVLIGKDRQFNRRF
                     LRMCGHYLVEPTACTPAAGWEKGQVENQVGNIRERLFTPRLRVASYEELNAWLLDQCI
                     AYARGNRHPEQTDRTIWQVFEAERAQLVPIGSRFDGFRATQASVSKTCLVRFDSNKYS
                     VASRAVGRPVEIQAYAERIVIRQDGAIVGEHARCFGRNQTVYDPWHYVPVLARKPGAL
                     RNGAPFKDWLLPAHLERVRRRLKGSDDGDRQMVKILSAVLTDGLAAVEAACAEALAGG
                     VHSADVVLNILARRRDPGTSATITAPEALRLRQAPIADCARYDRLRRAG"
     gene            1711..>2313
                     /locus_tag="VN968_27745"
     CDS             1711..>2313
                     /locus_tag="VN968_27745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043766072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL33061.1"
                     /translation="MERTDILELMSTLKLYGMRAAYDEVMTTGIKRRHEPPRIVGDLL
                     SAEIAEKQARSIKYQLTIAKLPLAKDIDDFNFAGTPINQTLIRDLAGGAFLAEQRNAV
                     LVGGTGTGKSHLAMAIARACIRGGARGRFYTVVDLVNRLENEARAGRQGRLAEYLTRM
                     DLIVLDELGYLPFAQAGGQLLFHLISRLYERTSVIVTTNLA"
BASE COUNT          386 a          754 c          779 g          394 t
ORIGIN      
        1 gacgatgtcg tcggtgaagc ccgtctgaaa gctttgctcg ctcgcctcga cgcggcgtgt
       61 cgtgggatcg gcctcgacga tcgtcgagct catcttctgt aaatcggcat ggaataaggc
      121 ccccgttttg gggtgatcgg catccaatcg ggacccccgg gacggcgggt tcacaatggc
      181 ttccatgggg gacatggggg ccgggtgggg gatgctgatc gtggagacga ttgcgaaggt
      241 ccgtcgggca tatttcattc agaagaagcc gatcaaggcg atctgccgcg agtttcgcct
      301 gtcgcgccga gtcgtgcgca aggtgatccg ctcggaatcg acggagttcc gctatgaacg
      361 cggccggcag ccactgccgc ggattgaccc gtggcgggac cagctcgacg ggctgttgct
      421 ggcgaacgag gagaagccgg cgcgcgagcg gctgaccctg attcgtgtct tcgaggagct
      481 cagcggtctc ggttatgagg gcggctatga cgcggttcgg cgctacgcca gggtttggcg
      541 ggcggcgcgc ggggccgcga cggcgcaagc ctatgtaccg ctgagctttg cgccgggcga
      601 agcctaccag ttcgactgga gccacgagat cgtcgtgatg aacggggtga cgacgacggt
      661 caaggtcgcc catgtccggc tgtgccacgg ccggatgccg ttcgtgcggg cctatccgcg
      721 cgagagccag gagatggttt tcgacgcgca tgcccgtgcc tttgatttct tcaagggcgc
      781 ctgcacgcgc ggcatctacg acaacatgaa gacggcagtc gagacggtgt tgatcggcaa
      841 ggaccgccaa ttcaaccgcc gcttcctacg gatgtgcggg cattacctgg tcgagccgac
      901 cgcctgcaca ccggcggcgg gctgggagaa ggggcaggtg gagaaccagg tcggcaacat
      961 ccgcgagcgc ctcttcacac cgcgcctgcg agtggcgagc tatgaggagc tgaacgcctg
     1021 gctgcttgac caatgcatcg cctacgccag gggcaaccgg cacccggagc agaccgatcg
     1081 tacgatctgg caggtcttcg aggcggagcg cgcgcagctc gtgccgatcg gcagccggtt
     1141 cgacgggttc cgcgcgacgc aggcttcggt ctccaagacc tgcctggtgc gtttcgacag
     1201 caacaagtac tcggttgcct cgcgtgccgt gggccggccg gtcgagatcc aggcttatgc
     1261 cgagcgcatc gtcatccgcc aggacggcgc gatcgtcggc gagcatgcgc gctgtttcgg
     1321 ccgcaaccag acggtctacg acccctggca ttacgtgccg gtgctggctc gcaagcccgg
     1381 cgcgctcagg aacggcgcgc ccttcaagga ctggctgttg ccggcccatc tggagcgcgt
     1441 gcggcgccgg ctgaagggtt cggacgacgg cgatcgccag atggtcaaga tcctgtccgc
     1501 ggtgctgacc gacggcttgg ctgccgtgga ggcggcctgc gccgaggccc tcgccggtgg
     1561 cgtccattcc gccgatgtcg tcctcaacat tctggcgcgc cgccgcgatc ccgggacgtc
     1621 ggcgacgatc acggcacccg aggcgctgcg cctgcgacag gcgccgatcg ccgattgcgc
     1681 ccgctacgat cgcctgcgca gggccggctg atggaacgca ccgatattct cgagctgatg
     1741 tcgacgctca agctctacgg gatgcgggcg gcctacgacg aggtgatgac gaccggcatc
     1801 aagcgccggc acgagccgcc gcgcatcgtc ggcgatctgc tcagcgccga gatcgccgag
     1861 aagcaggcgc gctcgatcaa gtaccagctg acgatcgcca aactgccctt ggccaaggac
     1921 attgacgact tcaacttcgc cggcacgcca atcaaccaga cgctgatccg tgacctggca
     1981 ggcggcgcct tcctcgccga gcagcgcaac gccgtgctgg tcggcggaac cggcactggc
     2041 aagagccatc ttgccatggc catcgcccga gcctgcatcc gcggcggcgc ccgcggccgc
     2101 ttctacaccg tcgtcgacct cgtcaaccgg ctcgaaaatg aagcccgcgc cggacgtcag
     2161 ggccggctcg ctgaatatct cacccgcatg gacctcatcg tgctcgacga actcggctat
     2221 ctgcccttcg ctcaggccgg cggccagctg ctctttcatc tgatcagccg gctctacgag
     2281 cgcacttccg tcatcgtcac caccaacctg gct
//
LOCUS       DATNOI010000695         4036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_286850,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000695 DATNOI010000000
VERSION     DATNOI010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286850"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VN968_27750"
     CDS             complement(<1..247)
                     /locus_tag="VN968_27750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HXL33062.1"
                     /translation="MRASYLFTSESVSEGNPDKVCDRISDEIVDLFFREGPKAGIDPW
                     AIRAACETLATTNKVVVAGETRGPSTVTFDQIESVVRE"
     gene            complement(304..1659)
                     /locus_tag="VN968_27755"
     CDS             complement(304..1659)
                     /locus_tag="VN968_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33063.1"
                     /translation="MKNDQILGLISEYCRQADMAETTFGRRAVNDGKLVHRLREGKRI
                     TIDTLDRIQAYIAASMPGGVPPPRGLEVPPEKRDPRGNFRFFENRQKYLLFVHTCSEK
                     RVIAERVALELGSIHPRPPALRVFDAGVGDGTVLARVMRSMHGRFPHMPFYIAGKELS
                     LEDVRLTLDKVPDRLFEHPATVFVLTNMYYAEAPWLTPASPAAAAGMIWHEVALRGAS
                     SGEFEAQIAELGPFLKQNWRANVSPRSGMPVYERPVAVVLYREDHRFLLDLIIPRAGR
                     SEANFDLIIASQPYRAKSSVNFRAKRIIAPLARALRAGGRLIGIHSHGQDPGLEIIQT
                     VWPAENPFSVSRHELLRAVKYELGSAGRDLNFNAYADNRSIFRYDMEALPNEVTGSIG
                     TSTAFAAWNAAVYVAQIEDDRLTEMTQSGRTLDATREVLRKHNGLWFYDESYVISRRR
                     D"
     gene            complement(1947..3128)
                     /locus_tag="VN968_27760"
     CDS             complement(1947..3128)
                     /locus_tag="VN968_27760"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016552666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXL33064.1"
                     /translation="MGQISHSLAEGALRNPDAIIVGAGPAGLACAMTMRGAGLNVTVL
                     EKADNVGSVWRRHYDRLHLHTDRKRSGLPGMVMPRTYPHYPSREQLIEYLEGYAVRFD
                     IRPVFNSTVSCIRRDGSQWRAETAQGSTNAPVMVIATGIAGAPYRPSWPGSEIYPGMV
                     VHSSEYRNPTPYAEKRVLVVGFGNSGGEIALDLATAGVDVALAVRGPVQILPRDLLGF
                     PILAWAILYRRLPARLVDAINAPILRLAVGRIEKLGLRRAAKGPRQMVEEDGRVPLID
                     IGTLDKIRDGSIKICGGIDRLTPDGVVFADGESRKFDAIILATGFRPDLRQLMPGLEG
                     VFDQHGMPLVTGQATNAPGLYFCGQITSPTGQLREIGLEAQRIAVFARRDRVAFSTRS
                     A"
     gene            complement(3116..>4036)
                     /locus_tag="VN968_27765"
     CDS             complement(3116..>4036)
                     /locus_tag="VN968_27765"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HXL33065.1"
                     /translation="FRRSWWHFDFLAKLTGCELFHPDTGIQFAGVETRHDAVRVIGKL
                     GFPDDHPMLDHFKFLKKQCEVAHVMPKMTIPSPAVLHFRGGRKSISKEVYPDLDVFYE
                     DLAKTYRKAVKAFYDAGCRYLQFDDTVWAYLCSQDELTKARERGDNPDGLQEIYARVI
                     NYAIAERPADMTITTHVCRGNFRPTWISSGGYEPVAETMLAGTNYDGYFLEYDSERAG
                     GFEPLRYLPKGDKIVVVGVITSKTGQLENKDDIKRRLEEASKFVPLDQLAVSPQCGFA
                     STEEGNILTEEEQWAKLKLAVDVAKEMWGK"
BASE COUNT          774 a         1270 c         1229 g          763 t
ORIGIN      
        1 cctcgcgtac cacgctttcg atctgatcga aggtgacggt cgaggggccg cgggtctcgc
       61 cggcgaccac caccttgttg gtggtggcca gcgtttcgca agccgcgcgg attgcccacg
      121 gatcgattcc cgctttcgga ccttcgcgga aaaacagatc gacgatctcg tcggaaatac
      181 ggtcgcagac cttgtccgga ttgccttcgg aaaccgactc tgaggtaaac agataagacg
      241 cgcgcatcaa ctaacccctt tccgccatat cccggcggtc tttcgtgatg ttgtcctgaa
      301 gtgtcagtcc cgacggcgcg agatgacgta ggattcgtcg tagaaccaga gcccgttgtg
      361 cttgcgcagt acctctctgg tggcatcgag ggtgcggccg ctttgagtca tttctgtcaa
      421 ccggtcgtct tcgatctggg cgacatacac cgccgcgttc catgctgcaa aggccgtcga
      481 ggttccgatc gagccggtga cctcattcgg cagcgcttcc atatcatagc ggaagatcga
      541 gcggttatcg gcataagcat taaagttcag gtcccgcccg gccgatccca gctcgtattt
      601 taccgcgcgc aatagctcat gacggctcac tgagaaagga ttttctgcgg gccacaccgt
      661 ctggatgatc tccagtcccg gatcctgccc gtgggagtga attccaatca aacggccgcc
      721 ggcccgcagc gcccgcgcca agggcgcaat gatgcgtttg gcgcggaaat tgaccgagga
      781 tttggcccgg tagggctgcg aagcaatgat caggtcgaaa ttggcctcgg agcggccggc
      841 gcgcgggatg atcaaatcga gcaagaatcg atggtcttcc cggtaaagta ccaccgcgac
      901 cggccgctcg tagactggca tgcccgagcg ggggctgaca ttggcgcgcc aattctgctt
      961 cagaaacggt cccaattcgg cgatctgggc ctcgaattcg cccgaggagg ccccccgcag
     1021 ggccacttcg tgccagatca tccccgcggc cgccgccggc gaggccggcg tcagccaggg
     1081 cgcctcggcg taatacatgt tggtaaggac aaagaccgtt gcaggatgct cgaacagccg
     1141 gtccggcacc ttgtccaagg tcagcctgac atcctcaagg ctgagctcct tgccggcaat
     1201 atagaacggc atatggggga agcggccgtg catcgaccgc atcacccgcg ccagcacggt
     1261 gccgtcgccg acgccggcgt cgaatacccg cagcgccggc ggccgggggt ggatactgcc
     1321 gagctccagc gccacccgct ccgcgatcac ccgcttttcg ctgcaggtat ggacgaacag
     1381 caggtatttc tgccgattct caaaaaaccg gaaattgccg cggggatcgc gcttctcggg
     1441 cggcacttcg aggccgcgcg gcggcggcac cccgcccggc atcgaggcgg cgatataggc
     1501 ctgaatccgg tcgagggtat cgatggtgat gcgcttgccc tcgcgcagcc ggtgtaccag
     1561 cttgccgtca ttgacggcgc ggcggccgaa cgtcgtctcc gccatatcgg cctgacggca
     1621 gtattcggag atcaggccga gaatctggtc gtttttcatc gggccgggat ttgggacaga
     1681 cgggtgggca gcggtggatt gcagggttgt cctaccacca tctgcccacc cttgggaaat
     1741 gcggggtgcg atacttggat agacaccgca ttcccgccac aacccggatc aaacaggtcc
     1801 cgcggtcaaa gcggcggctc cctcatggtg aggagcgcgc ccttgcgcgc gtctcgaacc
     1861 atgagacgca atatccatcc ttcgagacgg cgcggagttt atcatcgggc cgcgcgtcgc
     1921 gcggacccgt tggcgtctcc tcaggatcag gccgagcgtg tcgaaaacgc taccctatcg
     1981 cgcctggcga acaccgcgat tcgctgcgct tccagtccga tctcgcgcaa ttgccccgtc
     2041 ggcgaggtga tctgaccgca gaaataaagg cctggcgcgt tggtcgcctg ccccgtcacc
     2101 agcggcattc catgctggtc gaacacgcct tccaggccag gcatcaattg gcgcagatcc
     2161 gggcggaagc cggttgcaag aatgatcgcg tcgaattttc tgctttcccc atcggcgaac
     2221 acgacgccat cgggcgtgag gcggtcaatg ccgccgcata ttttgatcga gccgtctctg
     2281 attttgtcca gcgtgccgat atcgatcagg ggtacgcgcc cgtcctcttc gaccatttgc
     2341 cgcgggccct tggcggcgcg gcgcaatccc agtttctcga ttcgaccgac cgcgagccgc
     2401 aagatcggcg cgttgatcgc atcgaccaga cgcgcgggca agcgccggta caaaatggcc
     2461 catgccagga tcggaaagcc caggagatcg cgcggcagaa tctgaaccgg accgcgaacc
     2521 gcaagcgcaa catcgacgcc ggcagtcgcc agatccaaag cgatctcacc gcccgaattg
     2581 ccaaatccga cgaccagcac gcgcttttcc gcgtacggcg tcggattgcg atattcgctg
     2641 ctatggacga ccatccctgg ataaatttcc gaacccggcc acgaaggacg atagggtgcg
     2701 ccggctatcc ccgttgcaat gaccataacg ggcgcattgg tcgagccctg ggctgtctcc
     2761 gcgcgccatt gcgacccatc gcgccgaatg cacgatacgg tcgaattgaa aacaggcctg
     2821 atatcaaagc gtacggcgta gccttcgaga tactcgatta attgctcgcg agatggatag
     2881 tgcggatagg tccgcggcat caccatgccg ggcagaccgg agcgcttgcg atcggtatgc
     2941 aaatggagac ggtcgtaatg gcgtcgccag accgagccga cgttatcggc cttctcgagc
     3001 acggtaacgt tgagccccgc cccgcgcatc gtcatggcac acgccaaccc cgcggggccg
     3061 gcacctacga tgatcgcgtc gggattccgc aatgcgccct ccgcaagcga atgacttatt
     3121 tgccccacat ctctttcgcg acatcgaccg cgagtttcag cttcgcccac tgctcttcct
     3181 cggtgaggat gttgccctct tcggtcgagg cgaagccgca ttgcggcgat accgcgagct
     3241 ggtcgagcgg cacgaacttg ctcgcttcct ccagccgccg cttgatatcg tccttgttct
     3301 cgagttggcc ggtcttcgag gtgatgacgc cgaccaccac gatcttgtcg cccttcggca
     3361 aataccgcag cggctcgaaa ccgccggccc gctcggaatc gtattcgagg aaatagccgt
     3421 catagttggt gccggccagc atggtttccg ccaccggctc ataaccgccg gacgaaatcc
     3481 aggtggggcg gaaattgccg cggcaaacat gcgtggtgat ggtcatgtcg gcgggccgct
     3541 cggcgatcgc atagttgatc acgcgggcgt agatttcctg caggccatcg gggttgtcgc
     3601 cgcgctcgcg cgccttcgtc aattcatcct gcgagcagag ataggcccac acggtgtcgt
     3661 cgaactgcag ataacggcag ccggcgtcgt agaacgcctt cactgccttg cgataggtct
     3721 tggcgaggtc ctcgtagaaa acatcgagat cgggataaac ctccttcgag atcgacttgc
     3781 ggccgccgcg gaaatgcagc accgccggcg aggggatcgt catcttcggc atcacatgcg
     3841 cgacctcgca ctgcttcttc aggaatttga aatggtcgag catcgggtgg tcgtcgggga
     3901 aaccgagctt gccgatcacg cggaccgcgt catgccgggt ctcaacgcct gcgaactgaa
     3961 tgcccgtgtc ggggtgaaac agttcgcagc cggtgagttt cgccagaaag tcgaaatgcc
     4021 accacgagcg gcggaa
//
LOCUS       DATNOI010000696         4527 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_194549,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000696 DATNOI010000000
VERSION     DATNOI010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194549"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VN968_27770"
     CDS             complement(<1..993)
                     /locus_tag="VN968_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXL33066.1"
                     /translation="MTSPIAGGVDCDLHPAVPHLTSLLPHLNDYWRDQVTTRGMTDLV
                     SQSYPANSPISSRPDWRPAKGKPGSSLEDMQAQALEAFGVRYGICNPLYGVQMVFSED
                     MADAFCRALNDWLVKEWLDRDTRLRGSIVIPVQSIEKSVAEIERCAKDKRFVQVLMLV
                     MGDMPLGKRAYWPIYAAAERLGLPIGIHAGSNYHNPPTSVGWGSYHIEDYVAQAQAFQ
                     TQLTSLIVEGVFARHPNLKMVMLESGFTWLPSYLWRLHKFWRGIRMETPWVDRAPLEI
                     VRSNIRFSLQPIDAPPDPATLNRLFEHMQSDELLLFSTDYPHWQYEGDAVLPEGM"
     gene            1182..2138
                     /locus_tag="VN968_27775"
     CDS             1182..2138
                     /locus_tag="VN968_27775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL33067.1"
                     /translation="MAAAATMIALSGPVFAQQPAGSPPPLVDFAKVEIKTTALGDNCY
                     MLQGEGGNITVAVAKDGIIMVDSQYAPLHDKIKAAIEAISKQPIKYLIDTSFHGDHTG
                     GNQSFARDGVTVVSQTNVSKRLAAGTTNGLTGAKTPPAPQAALPSDTYTNFSKIRLSG
                     RVADLKHTPNAHTDGDTYVWFKTANVLSTGDTFTNRRYPEIDFANGGHINGTIAASDA
                     YLKLANAKTRIVPGHGPLADRAALMEYRTMLVTARERMAKLVKEGKSEDEVVAAKPFA
                     DLDAQWAPTELASKNFIRVVYHSLADKNAAKRPSFRRFFRRS"
     gene            2145..2627
                     /locus_tag="VN968_27780"
     CDS             2145..2627
                     /locus_tag="VN968_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HXL33068.1"
                     /translation="MKELVAIAEKIAARLIERRQTIAVAESSTGGLISAALLAVPGAS
                     AYFLGSAVVYTRDARRILMDIPDQAMKGIRSASEAYAKLLAGQIRQRFSSDWGLSETG
                     ATGPTGNRYGDAAGHSCIAVAGLEQSVITLETGSADRLANMHVFAATALDLLLQKLSR
                     "
     gene            complement(2788..4098)
                     /locus_tag="VN968_27785"
     CDS             complement(2788..4098)
                     /locus_tag="VN968_27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HXL33069.1"
                     /translation="MSGGEKRGARQSIRLHLIIGLAVVVILAGGLGGWASTAQISGAL
                     IAPGSVVVESNVKKVQHPTGGVVGEVRARDGDVVKAGDVVVRLDDTVTKASLAIVTKN
                     LDGLWARAARLEAEQRGLDNIVFPPMLLSRAEDPDVKAVIASETKLFEVRVNGRAGQK
                     AQLRERVTQLNEEIAGLTAQEKAKDKEIALVEKELVGVRSLYEQQLVQISRLTVLERD
                     AARLAGERAQYIASRAQAKGKITETELQIIQVDKDMVSEVSKDLRETNDKIGEFVERK
                     VTAEDQLRRVDIRAPQDGMVLQSTVHTVGGVITAGDAIMMIVPQADDLQVEAKVNPQD
                     IDKLQIGQKTLLRLSAFNQRTTPELNGVVTRVSPDVTTDQRTGQSYYTIRVSLPAEEV
                     ARLGDVKLIPGMPVEAFVQTGDRTMLSYLIKPLSDQLMRTFRER"
     gene            complement(4095..>4527)
                     /locus_tag="VN968_27790"
     CDS             complement(4095..>4527)
                     /locus_tag="VN968_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXL33070.1"
                     /translation="TIIAAAKEAGVHEMIIKMRDGYDTQIGEQGTALSAGQAQRVALA
                     RALYGNPFLIVLDEPNSNLDTEGDEALTRAVRAARERGAIVVVVAHRPIGIEAVDQLL
                     VLKDGRMQAFGPKETVLGQVLQRVAPPTPIKIVSDGGVAKS"
BASE COUNT          856 a         1450 c         1355 g          866 t
ORIGIN      
        1 catcccctcg ggcagaaccg catcgccctc gtattgccaa tgcggataat cggtcgagaa
       61 taagagcaat tcatccgact gcatatgctc aaacaggcga tttaatgtcg ccggatcggg
      121 cggcgcatcg atcggctgca acgaaaaccg gatgttgctg cgcacaattt ccaggggcgc
      181 acgatccacc cacggcgttt ccatgcggat gccgcgccag aatttatgca accgccacag
      241 ataggacggc agccaggtga agcccgactc cagcatcacc atcttcaggt tcggatgccg
      301 ggcgaagacg ccttcgacga tcaggctggt caactgggtc tgaaaagcct gcgcctgcgc
      361 gacgtaatcc tcgatgtgat aggaccccca gccgactgat gtgggcgggt tatgataatt
      421 gctgccggcg tggataccga tcggcaagcc cagccgctcg gcggccgcgt agatcggcca
      481 gtaggcgcgt tttcccagcg gcatgtcgcc catcaccagc atcaagacct gaacgaaacg
      541 cttgtccttg gcgcagcgct cgatctcggc gaccgatttc tcgatgctct gcaccgggat
      601 cacgatcgag ccgcgcagcc gcgtatccct gtcgagccat tccttgacca gccagtcgtt
      661 cagcgcgcga caaaacgcgt ccgccatatc ctcgctgaat accatctgca cgccgtaaag
      721 cggattgcag atgccgtagc ggacgccgaa ggcttcgagc gcctgcgctt gcatgtcttc
      781 gagacttgat cccggtttgc ccttcgccgg ccgccagtcc ggccgcgaag agatcggcga
      841 atttgccgga taggattgcg agaccagatc ggtcattcca cgcgtcgtta cctggtcgcg
      901 ccaataatcg ttgagatggg gcaacaggct ggtcaggtga ggaacggcag gatgcagatc
      961 gcaatccacg ccgcccgcga tcggtgacgt catcatcccg gtttcctctt gctcgcggcg
     1021 tctgtcgcgt tggtggcgca ccgcttgttc gaaatagcat atggcgcgcg ggctccgccg
     1081 tccatcggcg cgagttgccg atcccgagtc ggcagccatg caatcgtgtg ctaggacatc
     1141 tgaaaaaatc accgcggggg gtcgaaatgg acatggggag gatggccgct gcggcaacca
     1201 tgattgcgtt gtccgggccg gtattcgcac aacagccggc tgggtcaccg ccgcctctgg
     1261 tggatttcgc caaggtcgag atcaagacca ccgctctcgg cgacaactgc tacatgctgc
     1321 aaggcgaggg cggaaatatc accgtggcgg tggcgaaaga cgggatcatc atggtggaca
     1381 gccagtacgc gccgctgcat gacaagatca aggccgctat cgaagccatc tcgaagcagc
     1441 cgatcaaata tctcatcgat acgagctttc acggcgacca caccggcgga aatcaatcct
     1501 tcgccaggga cggcgtgacc gttgtctccc aaaccaacgt cagcaagcgc ctcgcagcag
     1561 gcactaccaa tggattgacg ggagccaaga ccccaccggc gccgcaggct gcgctgccgt
     1621 ccgatacata tacgaacttt tccaagatca ggctcagtgg ccgcgtcgcg gacctcaagc
     1681 acactccgaa cgcgcatacc gatggcgaca cctatgtctg gttcaagacc gcgaacgtat
     1741 tgtcgaccgg cgataccttc accaatcgcc gttatcccga gatcgatttc gccaatggcg
     1801 gccacatcaa cggcacgatc gccgcgtcag acgcttatct caagctggcg aacgccaaga
     1861 cccggatcgt tccgggccac gggccgcttg ccgacagagc ggcgctgatg gaataccgga
     1921 ccatgctggt caccgcgcgc gagcgcatgg caaaactcgt caaggagggg aaaagcgagg
     1981 acgaggtggt ggcggcaaaa ccgttcgcgg acctcgacgc acaatgggcg cccacggaat
     2041 tggcgagcaa gaattttatc cgcgtcgtct atcactcgct cgccgacaag aacgcggcca
     2101 agcggccatc attcagaaga ttcttccgca ggagttgaga acagatgaaa gaactggttg
     2161 cgatcgccga aaaaatagcg gcgaggctga ttgagcggcg ccagaccatc gcggtcgccg
     2221 aatcctcgac cggcggcttg atttccgcag cgctgctcgc ggtgcccggc gcctcggcct
     2281 acttcctcgg ctcggcggtg gtctataccc gcgatgcgcg gcggatcctc atggacattc
     2341 cggatcaggc gatgaaagga attcgttcgg cttcggaagc ttacgcaaaa ttgctcgcgg
     2401 gccagatccg ccagcgcttt tccagcgatt ggggcctgtc ggaaaccggc gcgaccgggc
     2461 cgaccggtaa ccgctatggc gatgccgccg gccattcctg catcgcggtt gcaggtctgg
     2521 agcagtcggt gatcaccctt gagaccggca gcgccgaccg gctcgccaac atgcatgtgt
     2581 ttgcggctac agcgctggat cttttgctgc agaagctgtc gcgctgacca ctccatagct
     2641 cgcgctatcg tcgtcccggc gaactcatac gccgtgtcgc ttgttttgag agaccttgtt
     2701 cgacgacttt cgcgcgacca cgaatgcctg tggttgggtc ccggcgttcg ccgggacgac
     2761 gtcgtggata atttcgcgtg agtcgactca cctctcgcgg aacgtgcgca tcaactggtc
     2821 gctcagcggc ttgatcaggt aggacagcat cgtgcggtcg ccggtctgca cgaaggcttc
     2881 gaccggcatg ccgggtatca gcttgacgtc gccgagacgg gcgacttctt ccgccggcag
     2941 cgaaacgcgg atggtgtaat agctttgccc ggtgcgctgg tcggtcgtga cgtcgggcga
     3001 aacgcgggtg acgacgccgt tcagttcagg cgtcgtgcgc tggttgaatg ccgacagccg
     3061 cagcagcgtc ttctggccga tctgcaattt atcgatatcc tgcgggttga ccttggcctc
     3121 gacctggagg tcgtcggcct gcggaacgat catcatgatg gcgtcgccgg cggtgatcac
     3181 cccgcccacg gtatggaccg tcgattgcag caccatgcca tcctgcggcg cgcggatgtc
     3241 gacgcgacgc agctggtctt cggccgtgac cttgcgctcg acgaattcac cgatcttgtc
     3301 gttggtctcg cgcaaatcct tggagacttc gctgaccatg tccttgtcga cctgaatgat
     3361 ctgcaactca gtctcggtaa tcttgccctt ggcctgcgcc ctcgatgcaa tgtactgcgc
     3421 ccgctcgccg gcgagccggg ccgcgtcgcg ctccagcacc gtcaggcgtg aaatctgcac
     3481 cagctgctgt tcgtagagcg agcgaacgcc gaccagttcc ttctccacca gcgcaatttc
     3541 cttgtctttc gccttttcct gcgccgtcag gccggcgatc tcctcgttca actgcgtgac
     3601 ccgctcgcgc aattgcgcct tttgtccggc ccttccgttg acacgaactt cgaacaactt
     3661 ggtttcgctc gcgatgacgg ccttgacatc ggggtcctcg gcacgagaga gcagcatcgg
     3721 aggaaatacg atgttgtcga ggccccgttg ttcggcctcc aaccgcgcgg ctcgcgccca
     3781 caacccgtcg aggtttttgg tgacgatcgc gaggctcgcc ttggtgacgg tgtcatcgag
     3841 ccgcacgaca acgtcgccgg ccttgacgac atcgccgtcg cgcgcgcgca cctcgccgac
     3901 cacgccgccg gtcggatgct gcactttttt tacgttggat tcgacgacga ccgatcccgg
     3961 cgcgatcagt gcccccgaga tctgggccgt cgacgcccag ccaccaagcc cgccggcaag
     4021 aatgacgacc accgccagac cgatgatcaa atgcagccgt atcgactgcc gcgctccgcg
     4081 cttttcgccg ccgctcatga tttggcaacg cctccatcgg atacgatctt gatgggagtg
     4141 ggcggcgcaa cccgctgcag cacctgcccg agaacggttt ccttgggacc gaacgcctgc
     4201 atgcggccgt ccttcaacac aagcagctga tcgacagcct cgattccgat cggccggtgt
     4261 gcgacgacca cgacgatggc gccacgctcg cgcgcggcgc gaaccgcccg tgtcagcgcc
     4321 tcgtctccct cggtatcgag attggagttc ggctcgtcaa ggacgataag gaacggattg
     4381 ccgtagagcg ccctcgccaa cgccacgcgc tgcgcctgac cggccgaaag cgcggtgccc
     4441 tgctcgccga tctgggtgtc gtaaccgtcg cgcatcttga tgatcatctc gtggacgccc
     4501 gcctccttcg cggcggcgat gatggtg
//
LOCUS       DATNOI010000697         6328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1082658,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000697 DATNOI010000000
VERSION     DATNOI010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082658"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VN968_27795"
     CDS             <1..1116
                     /locus_tag="VN968_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HXL33071.1"
                     /translation="TTLANTNSLCVTASADPLARLPAQCLLRGIPGTYTRLTAEAQWR
                     RSFTDSAGEIWTPFASVRADAIDASISNQPGVSNFLPVGDTQALRVMPTVGLEYRYPF
                     INVQPWGSTTIEPIAQVIIRPNEPYAGKLPNEDAQSLVFDTSNLFSVDKFSGYDRVEG
                     GGRANAGVQATTQFDRGGSVKVLFGQSYQLFGLNSFAVQDVTNTGVDSGLATPKSDYV
                     GSVNYSPNRTYTFSVRTRLDQQTLNVQRFEAEGRASFDRWSVSMMYGNYAPQPELGYL
                     TRREGLLSSASLKVAANWVVSGAARWDLEANKLNQYVIGAGYVDDCFVLAANYVTSYT
                     YAAGTTPPVLSHAFMLQIGLRTIANTATSANGASGIQ"
     gene            1201..2136
                     /locus_tag="VN968_27800"
     CDS             1201..2136
                     /locus_tag="VN968_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HXL33072.1"
                     /translation="MTTTLSHRFSVLVLTCAAAVALMSWASPSHAQTVAVMVNGEPIT
                     NYDIEQRSKLTFLTTHKPASRQQVVDELIDEKVKIKEGKKFGVDPTASDIDQSYAAMS
                     ARMRITPEQLTKSLEVQGIRADTLKARIKAEMVWTSLIRGRYKESLQVGEKDVAAAIR
                     VNGDEKQETEAFEYKMQPIVLIVPRGSAPATIEARQREAEALRSRVQTCADANAFFKS
                     MQNAAIRETVTKTSADIPAVLREVLDKTPVGHLTPPEVTKQGVEMVALCARKPTTVDT
                     PKKREIRDKMYAEKFQAKSKSYLQEVRKAAMVEYR"
     gene            2140..3144
                     /gene="pdxA"
                     /locus_tag="VN968_27805"
     CDS             2140..3144
                     /gene="pdxA"
                     /locus_tag="VN968_27805"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXL33073.1"
                     /translation="MARPLALTSGEPAGIGPDITIKAWLRRNELKLPPFYLLGDRDLL
                     SDRAKALGLNVKIGDVRAEDALAAFADTLPVVATGHAATARPGQPDHTSADAALGSIR
                     QAVADVMAGRAGAVVTNPIAKSVLYRAGFIHPGHTEFLAELAATGGHAPQPVMMLWSP
                     ALVVVPVTIHLALRDAIAQLSRELVVSTVRIVVADLKSRFGLANPRLAVSGLNPHAGE
                     GGSLGSEDQTIVAPAIDILRGEGIKIRGPLPADTMFHDAARKTYDCAICMYHDQALIP
                     VKTLAFEDAVNVTLGLPFIRTSPDHGTAFDIAGTGKANPSSLIAALRLAARMAMTQGA
                     "
     gene            3141..4007
                     /gene="rsmA"
                     /locus_tag="VN968_27810"
     CDS             3141..4007
                     /gene="rsmA"
                     /locus_tag="VN968_27810"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXL33074.1"
                     /translation="MSAIDDLPPLREVIRQHALSARKSLGQNFLLDLNLTARIARAAG
                     PLEDAIIIEIGPGPGGLTRALLALGARRVIAVERDQRALPALEQISRRYSGRLEVICA
                     DALHFDPRPLLGGRLAKIVANLPYNIATALLIDWLSIEPWPPWYDMMVLMFQREVAER
                     IVAHENDEAYGRLGVLANWRTETKILFDISPAAFVPQPKVTSSLVRLAPRSAPEPCDR
                     RALEQVATAAFGQRRKMLRQSLKSLAVDPARLAAAAHVDPTRRAETIPVSGFVAMARE
                     LSDIRKAKLNIP"
     gene            4020..5078
                     /locus_tag="VN968_27815"
     CDS             4020..5078
                     /locus_tag="VN968_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HXL33075.1"
                     /translation="MALMRRQSLVKFDAPLCETIVETPKPMAKEVLVRIERCGLCHSD
                     LHIQDGYADLGGGKKLDTTRGMTLPFTLGHEIAGVVEEVGPEVSNDLVGAKKAVFPWI
                     GCGQCRDCLNGDENLCVKQRFLGVAIDGGFASHVLVPDARYLLDYGPLPVNQAATLMC
                     SGVTAYGALKRLVDRPRQRNLLLIGLGGVGMMGLAFAQAMFKQKISVADLNPAARETA
                     LKNGADVAYDPSEPDVVKRMIRETEGGFDEIVDFAGNEKSMAFAVSALARGGKIVVSG
                     LMGGNFSLPMVQWIYKRMTIEGFMVGTLAEAHELMALARAGNIRPTPMREEPMGDVQK
                     WIDELRAGKVVGRIVLKN"
     gene            5321..6244
                     /locus_tag="VN968_27820"
     CDS             5321..6244
                     /locus_tag="VN968_27820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33076.1"
                     /translation="MQNRTAKFASAIVASLLAGTPLTTVSHSAVPAADDCLSGPKGQT
                     PQGSHWYYRIDRASKRHCWYLADEREKLSRAKPQNSAPIADPASPQKETAAQRPVADA
                     HAELPLPQTRVEQETSVSAWQGIPATTANAASVENGQAANALDAKTRPSVIASRWPDP
                     SGVSSLVSPTPTTGNSGEAVQLNTENTEQAPLPAVGTSAAVDPSSEKQSGSVQMLLLV
                     MIGALVLAGVMGSAIFRFGRIRQAGRRQNRSDRRAIWDSVDTERPSPPAYPRAEASMR
                     GVDIPRELRQADDPNGRIAEMLARLSKGAAT"
BASE COUNT         1259 a         1972 c         1956 g         1141 t
ORIGIN      
        1 acgacgctgg ctaacaccaa tagcctctgc gtgacggcgt ccgccgatcc gctcgcgcgg
       61 ttgccggcgc aatgcctgct tcgcggcata cccggaacgt acacgcggct tacggctgag
      121 gcgcagtggc ggcggtcgtt caccgattcc gccggtgaaa tctggacgcc gtttgcgagc
      181 gttcgcgcgg atgcgatcga tgcctcgatc tcaaaccagc ccggcgtttc gaacttcctc
      241 ccggtcggcg acacccaagc gctgcgcgtg atgccgacgg tcggcctcga atatcgctat
      301 cccttcatca acgttcagcc ttggggttcg acgacgatcg agccgatcgc gcaggtcatc
      361 attcgtccca acgaacccta tgcgggcaag cttcccaacg aagacgccca gagtctggtt
      421 ttcgacacca gcaacttgtt cagcgtcgat aaattctcgg gctatgaccg cgtcgaaggc
      481 ggcggccgcg ccaacgccgg cgtgcaggcg accacgcaat tcgaccgcgg aggttccgtc
      541 aaggtattgt tcgggcagtc ctatcaactg ttcggcctga attcgttcgc cgtccaggat
      601 gtgaccaata ccggcgtcga ttccggcctg gcgactccca agtcggacta tgtcggcagc
      661 gtcaactatt cgcccaaccg aacctatacg ttcagcgtgc gcactcgcct ggaccagcaa
      721 actcttaacg ttcagcgttt tgaggctgaa ggccgcgcaa gcttcgaccg ctggtcggtc
      781 agcatgatgt acggcaatta tgcgccgcaa ccggaactgg gatacctgac ccgtcgcgag
      841 ggacttttga gcagcgcgtc cctcaaggtg gcggcgaact gggtggtgtc gggagctgcc
      901 cgatgggatc tcgaagcgaa caagctcaat cagtacgtca tcggtgccgg gtacgtggac
      961 gactgctttg tgctggcggc gaattatgtc acttcctaca cttacgccgc cggcaccacc
     1021 ccgcctgtgc tcagtcacgc cttcatgctg cagatcggct tgcggaccat cgccaatacg
     1081 gccacgtccg ccaacggggc ctcagggatc cagtagaacc ggcgccggca cgctgccgga
     1141 ggatgatttt ccggcgcgtc actggtccaa ttcgaatcgg ttgatatagc gaacatgatc
     1201 atgacgacta cactctctca tcggttttcg gtcctcgtcc tgacttgtgc cgcggcagtg
     1261 gccctgatga gctgggcctc gccgtcgcat gcgcaaaccg ttgccgtcat ggtcaacggc
     1321 gaacctatca ccaactacga tatcgagcag cgcagcaagc tgaccttcct gacaactcac
     1381 aagcctgcca gccggcagca agtggtcgac gaactgatcg atgaaaaggt aaagatcaag
     1441 gaaggcaaga aatttggcgt cgaccccacc gcttccgata tcgatcagtc ttacgcggcg
     1501 atgagtgcgc gaatgcgaat cacgccggag caactgacaa aatctctgga agttcagggt
     1561 atccgggccg acacgctgaa ggcccgcatc aaggccgaga tggtatggac cagcctgatc
     1621 cgcgggcgat acaaggagag cctgcaggtc ggcgaaaagg acgtcgccgc cgccatccgg
     1681 gtcaatggcg acgaaaagca ggagaccgag gccttcgaat acaagatgca gccgatcgtt
     1741 ttgatcgtgc cgcggggatc ggcacccgcc accatcgagg caagacaaag ggaggcggaa
     1801 gccctgcgca gccgcgtgca gacctgcgcc gacgccaacg cgttcttcaa gtcgatgcaa
     1861 aatgccgcga tacgcgagac cgtgaccaag acctcggctg acataccggc ggttcttcgc
     1921 gaagtcctgg acaagacacc ggtcggccat ttgactccac ccgaggtgac gaaacagggc
     1981 gtggagatgg ttgcgctgtg cgcaaggaag ccaaccaccg tcgacacgcc gaagaagagg
     2041 gaaatccggg acaagatgta tgccgagaaa ttccaagcga aatcgaaatc gtatttgcag
     2101 gaagtccgca aagccgcgat ggtcgaatat cgttgacgca tggccaggcc cctcgcgctg
     2161 acatccggcg agcccgcggg tatcgggccc gatatcacca tcaaggcgtg gctgcggcgc
     2221 aatgagctga agctcccccc attctatttg ctcggcgacc gtgacctgct cagtgaccgg
     2281 gcgaaggctc tcggacttaa cgtcaagatc ggcgacgttc gcgccgaaga tgcactcgct
     2341 gcattcgccg acacgttgcc ggtcgtggca accggccatg cggcaaccgc gcggccgggc
     2401 cagccggacc ataccagcgc ggacgcagcc cttggctcga tcaggcaggc cgtcgccgat
     2461 gtcatggccg gacgggctgg cgcggtcgtc accaatccga ttgccaagag cgttctctac
     2521 cgcgccggat tcatccatcc cggccatacc gagtttctcg ccgagctcgc ggcgaccggc
     2581 ggccatgcgc cacagccggt aatgatgctg tggtcccctg ccctcgtcgt cgtgcccgtg
     2641 accatccatc ttgcgctgcg tgacgccatc gcgcagctgt cgcgtgagct ggtcgtcagc
     2701 accgtccgga tcgtggtcgc ggatctgaaa agccgcttcg gccttgccaa tccgcgcctt
     2761 gctgtatccg gactcaatcc gcacgctggc gaaggcggct cgcttggcag tgaggaccaa
     2821 accatcgttg cgccggcgat tgatatcctg cgcggcgaag gcatcaagat cagggggccg
     2881 ctgcccgcgg acaccatgtt ccacgatgcc gcgcgcaaaa cctatgactg cgcgatctgc
     2941 atgtatcacg accaggcgct aatcccggtc aagacgctgg cgttcgagga cgccgtcaac
     3001 gtcacgctgg gattgccgtt cattcgaacc tcgcccgacc atggcacggc gtttgacatt
     3061 gccggcaccg gtaaggccaa tccgtccagc ctgattgcgg cattgcggct ggccgcccgc
     3121 atggcgatga cgcagggcgc atgagcgcga tcgatgacct tccgccgctt cgcgaggtca
     3181 ttcgccagca tgccctgtcg gcccgcaaat cgctcggcca gaatttcttg ctggacctca
     3241 acctcaccgc acggatcgcg cgcgccgcgg gcccactcga ggatgcgatc atcatcgaga
     3301 tcggccccgg ccccggcggg ctgacgcggg cgctgctggc gctgggcgcg cggcgcgtca
     3361 tcgccgtgga acgcgaccag cgcgcgctgc cggcgctcga gcagatctcg cggcgatatt
     3421 cgggacggct ggaagtcatc tgcgccgacg cgctgcattt cgatccacgg ccgctgctcg
     3481 gcggcaggct tgcgaaaatc gtcgccaacc tcccctacaa catcgcaacc gcgcttctga
     3541 tcgactggct gtcgatcgag ccgtggccgc cctggtacga catgatggtg ctgatgtttc
     3601 agcgcgaggt ggctgagcgg atcgtcgcgc acgagaacga cgaggcctat ggacggctgg
     3661 gcgtgctcgc gaactggcgt actgaaacaa aaatcctgtt cgacatttct ccggccgcgt
     3721 tcgtgccgca gccaaaagtc acctcttcgc tggtacgcct ggcgccccgc agcgcgccgg
     3781 aaccctgcga ccgccgggcg ctcgagcagg tcgctacggc ggcgttcggg cagcggcgca
     3841 agatgctgcg gcaaagcctc aaatcactgg ctgtcgatcc ggcgcggctg gccgctgccg
     3901 cgcatgtcga tccgacgcgg cgtgccgaaa ctattccggt cagcggcttt gttgccatgg
     3961 cccgtgaatt gtccgatata cgaaaagcaa aattgaatat tccctaggga gaagacagca
     4021 tggcgctgat gcgacgccaa tcattggtca aattcgatgc gcctttgtgt gaaaccatcg
     4081 tcgagacgcc gaagccgatg gctaaggaag tcctggtccg tatcgagcgc tgcgggctgt
     4141 gccactccga tctgcatatt caggacggtt acgccgacct cggcggcggc aagaagcttg
     4201 ataccacgcg cggcatgacg ctgccgttca cgctgggaca cgagatcgcg ggcgtcgtcg
     4261 aggaagtcgg ccccgaggtt tcgaacgacc tggtcggcgc caagaaagcg gtcttcccgt
     4321 ggatcggctg cggccaatgc cgcgactgcc tgaatggcga cgagaatctc tgcgtcaagc
     4381 agcgttttct cggcgttgcc atcgatggcg gttttgcgag tcacgtgctg gtgccagacg
     4441 caagatatct gctggattac ggcccgttgc cggtcaatca ggcagccacc ttgatgtgtt
     4501 ccggcgtgac cgcctatggc gcgctgaagc gcctggtcga ccggccgcgg caacgcaatc
     4561 tcttgctgat cggccttggc ggcgtcggca tgatggggct ggctttcgca caagccatgt
     4621 tcaagcagaa gatttcggtg gcggatctga atcccgccgc gcgcgagacg gcgttgaaaa
     4681 atggcgctga tgttgcctac gatccttccg agcccgacgt ggtcaaacgc atgatcagag
     4741 aaaccgaggg aggtttcgac gagatcgtcg attttgccgg taacgaaaaa tcaatggcgt
     4801 ttgcggtctc cgcgctcgcg cgcggcggca agatcgtggt ctccggactg atgggcggca
     4861 atttcagcct gccgatggtg cagtggatct acaagcgcat gacgatcgag ggattcatgg
     4921 tcggaacatt ggcggaagcg catgagctga tggcgctggc gcgcgccgga aacatcaggc
     4981 cgacgccgat gagagaagag ccgatgggcg acgttcagaa atggatcgac gagctgcggg
     5041 ccggaaaagt cgtcggccgc atcgtgctga aaaactgagc cggagcgttt cgctctgttc
     5101 agcgccgtgc tcgcggcaag tgacgtagct tttcggaaaa gcgcgggggc gctcttcgca
     5161 gccccaacat ttatctgcgg ctgggccttt gcgatgcgaa agtgagcccg aattaagcct
     5221 catgctgtcc gaaattgcgg ctcattgcga cccaagttct cgcggagaac ctggggccaa
     5281 agatatcgcg catcgatcaa gtcatcccgg agatgcaaac atgcaaaacc gaaccgcgaa
     5341 attcgcatca gcaatcgtcg ccagcctcct cgcgggcacc cctctcacaa cggtatcgca
     5401 cagcgcagtg cctgccgccg acgactgcct gtccggaccg aagggccaga cgccgcaagg
     5461 cagccattgg tactaccgta ttgatcgtgc cagcaaacgc cattgctggt acctcgccga
     5521 cgagcgcgaa aagctttccc gggccaagcc acaaaattcg gctccgatcg cagaccctgc
     5581 ttcgccacag aaagagacgg ctgcgcagcg gccggtcgca gatgcgcatg ccgaactgcc
     5641 cttgccacag acgcgggtcg agcaggaaac aagcgtctcc gcttggcaag ggattccagc
     5701 gacaaccgcg aatgcggcga gcgttgagaa cggccaggct gcgaatgcac tggatgcgaa
     5761 aacgcggccg tcggtcatcg cttcgcgctg gcccgacccg tcgggcgtga gttcgttggt
     5821 cagtccaaca ccgacgacag gcaattcggg tgaggctgtg caattaaata cagagaatac
     5881 ggagcaagca ccactgcccg ccgtcggcac ttcggccgca gtggacccgt catccgagaa
     5941 acagtccggc tcggtccaga tgctgttgct cgtcatgata ggcgcactgg tgcttgccgg
     6001 ggtcatggga agcgcgattt tcaggttcgg cagaatacgg caggctggcc ggcggcaaaa
     6061 tcgaagcgat cggcgcgcga tctgggattc ggtggatacc gagcgcccgt cgcctccggc
     6121 ttacccgaga gcagaggcgt caatgcgggg agttgatatc ccgcgcgagc tgcgtcaggc
     6181 ggatgatccg aacggcagga tcgcggaaat gctggcacga ttgtccaaag gcgccgcgac
     6241 ctgacgtcct gctatttccc taattgccgc tgcaactcgc gcacgaacgt cgtcaaacct
     6301 gtgcgacgtt cgcgcttcag ccgctcgg
//
LOCUS       DATNOI010000698         4854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_421507,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000698 DATNOI010000000
VERSION     DATNOI010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421507"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..755
                     /locus_tag="VN968_27825"
     CDS             183..755
                     /locus_tag="VN968_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HXL33077.1"
                     /translation="MTVPQDINKLQSEVSAAVREHWKAFLFEGILLVVLGLAAMILPP
                     LASLAVTIFLGWMFLISGAAGLALTFWARQMPGFWWSLVSAALAVLAGIVLLTRPVQG
                     VLTLTIVIGAYFLAEGVTTIMYALEHRRELSERWSWLLFAGVLDILIAAMIITGLPGS
                     AEWAIGLLVGINLLFGGASLIGMALAARKG"
     gene            979..2064
                     /locus_tag="VN968_27830"
     CDS             979..2064
                     /locus_tag="VN968_27830"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXL33078.1"
                     /translation="MLSTTDDLRIEQIKELSTPAEVTREIPRTLTATRIVMAARNAIH
                     AILHGTDDRLLVVVGPCSVHDPAAAVDYAERLATLREGLVERIEIVMRVYFEKPRTTV
                     GWKGLVNDPDLDGSFNINKGLRLARNVLSAVNNLGLPAATEFLDMTTPQYIADLVAWA
                     AIGARTTESQIHRELASGLSCPVGFKNGTDGNVRIAAEAVKSASHPHHFMAVTKGGRS
                     AIAATSGNDDCHIILRGGSKPNYDSASVASAAAELARAGVAQRMMIDTSHANSGKKPE
                     NQPLVIADIARQISDGEQRIVGVMIESNLVAGRQDVLTGTALTYGQSITDGCIDWNTT
                     VPALNLLADAVASRHDMLRRKCREYSA"
     gene            complement(2134..2394)
                     /locus_tag="VN968_27835"
     CDS             complement(2134..2394)
                     /locus_tag="VN968_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33079.1"
                     /translation="MRKLIMSMALLALIGAPAAMAQTGNKTGTSGAANQPPQPFREAP
                     IGHRQPRADEVPSEKNLSNPDTPANREDAALDRKIKSICRGC"
     gene            complement(2469..3599)
                     /locus_tag="VN968_27840"
     CDS             complement(2469..3599)
                     /locus_tag="VN968_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HXL33080.1"
                     /translation="MSRINLKPAMTLREPAELVAHGLAPAADLAGLEQVAARYAVAVT
                     PDIAALIDTDDPDDPIARQFIPRAEELVAQPGESADPIGDHAHSPVAGIVHRYPDRVL
                     FKLVHVCAVYCRFCFRREMVGPGKATALSSDAYRGALDYIREHRGIWEVILTGGDPLM
                     LSPRRLAEIMADLAAIDHVRIIRIHTRVPVADPARISAEMIAALRVKGAATWVALHAN
                     HAKELADKARAACAAMIDAGIPMVSQSVLLRGVNDNAAALEALMRAFVECRIKPYYLH
                     HGDLAPGTAHLRTTLAEGRELMRALRGRVSGLCQPEYVLDIPGGHGKAPVGPNYLSHA
                     NPFLETNSFLEQRERQAETRYRIVDYCGDVHLYPPEPSLKDD"
     gene            complement(3596..4648)
                     /gene="epmA"
                     /locus_tag="VN968_27845"
     CDS             complement(3596..4648)
                     /gene="epmA"
                     /locus_tag="VN968_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HXL33081.1"
                     /translation="MADDDQLSPWWSATRHADIRPFLAARSAITKAIRAWFDEQGFAE
                     VETGILQVSPGNETHLHAPRTELTSATGALATRYLRTSPEFACKKLLAAGEPRIFEFA
                     RVFRDRERGDLHLPEFTMLEWYRADAAYDAVMADSVVIIAHAAQATGIGRFLFRGRSA
                     DPFAEPELLTVAAAFERFAGIDLLATIAGDEGDRAGLAAAAGTRVRIMDDDTWSDIFS
                     KVLVEHVEPNLGQGRLTVLFEYPAPEAALARAKASDPRVAERFEIYACGVELANGFGE
                     LTDAEVQHRRFMQAMDEKARRYGERYPLDEDFLAAVAKMPEASGVALGFDRLVMLAGG
                     ALRIDQVVWTPPAGEA"
     gene            4703..>4854
                     /locus_tag="VN968_27850"
     CDS             4703..>4854
                     /locus_tag="VN968_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXL33082.1"
                     /translation="MLQHETASLASGRPCPDRSRARISGKTALKVIASSIRKGNVIEQ
                     DGKLYVV"
BASE COUNT          916 a         1548 c         1473 g          917 t
ORIGIN      
        1 accacggcga tctatattct cggtcgcaat cggatgcgca cgacgtcccg ggacttttcg
       61 tccgcgcccg gccgaggcta ggacagaacc gtttcgcttg aacgatgtga acaaatttaa
      121 ccgcccggcg ctggcgcagg ctatattgtg gttctgcgat accagtatcg ggagattttc
      181 caatgaccgt tccccaggac atcaacaagc tgcaatccga agtgagcgcc gccgtgcgcg
      241 agcattggaa ggcctttttg tttgaaggca tcctgctcgt ggtgttgggt ctcgcagcca
      301 tgatcctgcc gccgctcgcc agccttgccg tcaccatctt tctgggttgg atgtttctga
      361 tcagcggcgc cgccgggctg gcgctgacgt tctgggcccg gcaaatgccc ggcttctggt
      421 ggtcgctggt ttcggcggcg ctggctgttc tcgccggcat tgtgctcctg accaggcctg
      481 tgcagggcgt actcaccctc acgatcgtga ttggggcgta tttcctggcc gaaggcgtca
      541 ccaccatcat gtacgcgctg gaacatcgcc gcgaattgtc ggaacggtgg agttggctgc
      601 tgttcgctgg cgtcctggac atcctgattg ccgccatgat catcaccggg cttccgggtt
      661 cggcggaatg ggcgataggc ctgctggtcg gcatcaatct gctgttcggc ggcgcgtcgc
      721 tgatcggaat ggcgctcgcc gcgcgcaagg gctgaaccag cccgattttg agatgacaac
      781 ggacaatgca tgcgctatag cgcgtgccat gatcaccgtt gccaccagca atttttggta
      841 ttttagcttc gacagctcgc aggcggcggg aggatcacgc tcaatctgaa gaattgcagc
      901 aggaagtccg aacagccgcc cgacttggcg gctttttgtt tggccggcag gtcctcaaaa
      961 cacacaggag ccacagccgt gttgagcacc acagacgatc ttcgaataga gcaaataaaa
     1021 gagctgagta cgccggcaga agtcacgcgc gaaataccgc gcaccttgac cgcgacgcga
     1081 attgtgatgg cggcgcgcaa cgccatccac gcgatccttc acggaacgga cgatcgcctt
     1141 ctcgtcgtcg tcggtccttg ctcggtccac gatccagccg cggccgtgga ctacgccgag
     1201 cgcctcgcta ccttgcgcga ggggcttgtc gagcggatcg aaattgtcat gcgcgtctat
     1261 ttcgagaagc cgcgcacaac ggtggggtgg aagggcctcg tcaacgatcc cgacctggat
     1321 ggcagcttca acatcaacaa gggcctgcgt ctcgcccgca acgtgctttc cgccgtgaac
     1381 aatcttggtc tgccggcggc gaccgaattc ctcgatatga cgacgcccca gtatatagcc
     1441 gatctggttg cgtgggcagc catcggcgcg cggacaaccg aaagccaaat tcatcgcgaa
     1501 ctcgcttccg gactttcctg tccggtcggc ttcaagaacg gcaccgacgg caatgtgcgc
     1561 atcgccgctg aggcggtgaa gtcggcatcg catccccacc atttcatggc cgtcaccaag
     1621 ggtggacgtt ccgcgatcgc cgcgacatca ggcaatgacg actgtcacat cattctgcgt
     1681 ggcggcagca agccaaatta tgattccgcc agcgtcgctt ccgccgcggc cgaacttgcc
     1741 cgtgcaggtg tcgctcagcg catgatgatc gacacgagcc acgccaacag cggcaagaag
     1801 ccggagaacc agccactggt gattgccgat attgcccgcc agatatccga cggcgagcag
     1861 cgcatcgtcg gagtcatgat tgaaagcaat ctggtggcgg gccgccagga tgtgctgacc
     1921 ggcacggcgc tgacctatgg gcagagcatt acggacggct gcattgactg gaacacgacc
     1981 gtgccagcac tcaacctgct ggctgacgcg gtcgcgtcga gacacgatat gctgcgccgc
     2041 aaatgccggg agtattcggc ctgagtcggt tgtgctgatc gatcgtcatc ggggcgtgtc
     2101 ggttcgcgcc ctgccggccg ccaccagcgc tctctagcag ccgcggcaga tgctcttgat
     2161 cttgcggtcg agcgccgcat cctcgcggtt ggcgggagtg tccggattgc tcaggttttt
     2221 ctccgacggc acctcatcgg cgcgcggttg ccggtggccg atcggggctt cgcggaaggg
     2281 ctgcggcggc tgatttgcag ctcccgacgt tccggttttg ttgccggtct gcgccatcgc
     2341 cgcgggagcg ccaatcaggg ccagtagcgc catcgacatg atcagctttc gcatctgaat
     2401 tctccatcaa cagagaaacg catctacctg gcaggatcgg gagcctctcc cgtcctgccc
     2461 ctcctccgtc agtcatcttt caatgatggc tcgggaggat agagatgaac gtcgccgcaa
     2521 tagtcgacga tacgatagcg cgtttccgcc tgccgttcac gttgttccaa aaaagaattt
     2581 gtctccaaaa aaggatttgc atgtgacaaa taattcggac cgaccggggc cttgccatgg
     2641 ccgccgggaa tatcgagcac gtattccggc tggcacagcc cggagacccg cccccgcagt
     2701 gcacgcatca attcccggcc ctccgccagc gtcgtgcgca gatgcgccgt gccgggcgcg
     2761 agatcgccgt ggtgcaggta atagggcttt atccggcatt cgacaaaggc ccgcatcagc
     2821 gcttccagcg ccgcggcatt gtcattgacg ccgcgcagca gcaccgactg gctgaccatg
     2881 gggattccgg cgtcgatcat tgcagcgcac gcggcgcggg ccttgtcggc caattctttt
     2941 gcgtggttgg cgtgcagtgc aacccaggtc gcagccccct tgaccctgag cgccgcgatc
     3001 atctccgcgc tgatgcgggc gggatccgcc accggcacgc gggtgtggat ccggatgatc
     3061 ctgacgtgat cgatggcggc gaggtccgcc atgatctcag cgagccgccg tggcgatagc
     3121 atcagggggt cgccgccggt caggatgacc tcccagatcc cgcgatgctc ccggatatag
     3181 tcgagcgcgc cgcgataggc gtcggacgag agcgcggtcg ccttgccagg cccgaccatt
     3241 tcgcggcgaa aacagaaccg gcaatacacc gcgcagacgt gaaccagctt gaacagcacc
     3301 cgatcgggat agcgatgcac gatgccggcg accggcgaat gcgcgtgatc gccaatcgga
     3361 tcggcgctct cgcccggctg cgccaccaat tcctcagcac gtggaatgaa ctgtcgcgcg
     3421 atcggatcgt cgggatcatc cgtgtcgatc aaggcggcaa tatccggcgt caccgccact
     3481 gcatagcgcg ccgcgacctg ttccagaccg gcaagatccg ccgcaggtgc caggccgtgg
     3541 gcgaccagct ctgcgggctc acgcagcgtc atggcgggct tgagattgat cctgctcatg
     3601 cctcaccggc cggcggcgtc cataccacct gatcgatccg cagcgcgccg ccggccagca
     3661 tcaccaaccg gtcgaagccg agcgcgacgc cgctcgcttc cggcatcttt gccacggcag
     3721 cgagaaaatc ctcgtcgagc ggatagcgct cgccgtagcg ccgcgccttt tcgtccatgg
     3781 cctgcatgaa acgccggtgc tgcacctcgg catcagtcaa ctcaccaaat ccattggcaa
     3841 gttcaacgcc gcaggcataa atctcaaaac gctcggcgac gcgcgggtcg gacgctttgg
     3901 cgcgcgccag cgccgcctcg ggcgccggat attcgaacaa caccgtcaag cgcccctgcc
     3961 ctagattggg ttcgacgtgc tcgaccagca ccttgctgaa gatatccgac caggtgtcgt
     4021 cgtccatgat ccgcacccgt gtcccggccg cggccgccag gcctgcgcga tcgccctcgt
     4081 ctcctgcgat ggtggcgagc agatcgatac cggcaaagcg ctcgaacgcc gccgccaccg
     4141 tcaacagttc cggctcggca aatggatcgg cgctgcggcc gcgaaacaaa aatcgcccga
     4201 ttccggttgc ctgcgcggca tgggcgataa tgacgacgct gtcggccatc acggcgtcat
     4261 aggccgcatc ggcacggtac cattccagca tggtgaattc gggaagatgc agatcgccgc
     4321 gctcccggtc gcgaaatacc cgcgcaaact caaagatcct gggctcgcct gcggccagca
     4381 atttcttgca ggcgaattcg ggcgaggtcc gcaagtaccg cgttgcaaga gcgccagtgg
     4441 cggaggtcaa ttcggtgcgc ggcgcatgca ggtgggtctc attccccggc gagacctgca
     4501 gaattccggt ttcgacctcg gcaaaaccct gttcgtcgaa ccatgccctg atcgccttgg
     4561 tgattgcgct tcgcgccgcc aaaaatggcc ggatgtcggc gtgccgcgtc gccgaccacc
     4621 atggcgaaag ctgatcgtcg tcggccatca gcgaactgcc tcaaaaaccc gcaaaacgct
     4681 ggcatcgact ggcaaaatca gtatgttgca gcacgaaacc gcttcgctgg cctcgggacg
     4741 cccttgtccg gatcggtccc gggccagaat atcaggaaaa acagctttga aagtcatcgc
     4801 cagttctatt cgcaagggca acgtcatcga gcaagacggc aagctttacg tggt
//
LOCUS       DATNOI010000699        16356 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1289863,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000699 DATNOI010000000
VERSION     DATNOI010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 16356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16356
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289863"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3366
                     /gene="putA"
                     /locus_tag="VN968_27855"
     CDS             <1..3366
                     /gene="putA"
                     /locus_tag="VN968_27855"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HXL33083.1"
                     /translation="PDAQTMDDLIHDKIAPHDWSSHSGSSSSVFVNASTWALMLTGEV
                     LDDSDSGIEGTLRGVIRRLGEPVIRKAMSAAMREMGEQFVLGRTIDEAMRAGRDVTRK
                     GYLYSFDMLGEAARTERDALRYHRAYADAIASIGAAADNVDSNMNHGISVKLSALHPR
                     YEVGQKDTMLPVIVQRVLSLALAARDARIGLNIDAEESDRLDLSLGIIEQVLSDSRLA
                     GWKGMGVVVQAYGPRAAFVIDWLYALAERLDRRIMVRLVKGAYWDTEIKRAQVLGLDG
                     YPVFTRKPNTDVSYIACAKKLLGRTGRIYPQFATHNAHTVAAILAMAGDRESFEFQRL
                     HGMGEALHEAARATEGTRCRIYAPVGAHSELLAYLVRRLLENGANSSFVHQIVNKTIE
                     AEDIVHDPFESVMTQGPAANPAIPPPARIFGAGRRNSRGFDLADPVTLARIDAETKPF
                     AAPHLWNAGPIVAASFLGGRARSVLNPARPDERVGEVVDAPAADVARAVDAGVRARTG
                     WERTSVGMRADMLVKAADLYEQNAAEFFALCAREAGKTLGDAVSEVREAVDFLRYYAG
                     QAANAEHDTGARGLIVCISPWNFPLAIFTGQIAAALVTGNAVVAKPAEQTPLIAARAV
                     ELLHQAGIPKDVLQLLTGDGSVGAALTADPRIDGVCFTGSMEVARLIEQQIAATAEPD
                     AMLIAETGGLNAMIVDSTALPEQSVRDILASAFQSAGQRCSALRILYVQKDVEQKTLE
                     MLKGALAMLRIGDPWLISTDVGPVIDDEARGALKAYCAEMEKKGRLIAKLDAPREGCF
                     VGPHIFRVSGITEMEHEMFGPILHVATFEADMIDSVIAEINAKQYGLTFGLHTRIEAR
                     VKHLVESIHAGNIYVNRNQIGAVVGSQPFGGEGLSGTGPKAGGPQYLRRFRKGLTTAV
                     NAAEAPVVSASTLAEHMPDATLGGWSTRPDRLAVLRKHLRGKGAEAITAAAAIDFGPV
                     DLPGPTGEANTLLRAPRGRVLCLGLDEAAVLAQSVQALAAGNAVLAVAPRAQACLLPL
                     LDKGLPIRAINGTVDAGDLVQLSVEVVAISADVDTLRAIRRALARRSGPIVPLVSEVI
                     SPNAYVHERAVCVDTTAAGGNATLLAAAEPINV"
     gene            3800..4756
                     /locus_tag="VN968_27860"
     CDS             3800..4756
                     /locus_tag="VN968_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL33084.1"
                     /translation="MRSYVVRRLLALVPTLLFASLIVFVIVRLVPGSIIDMMLSQNDV
                     GADKLSRDQLIQTLGLDRPMWEQYFRWIGRILLHGDLGRSLWQNTPVAELLRARLPIT
                     FQLGLMALIVALLIAIPIGIYSAIRQDTAGDYVTRSFSILMLAVPSFWMGTMVMVFPS
                     IWWGWSPEVKFVAFTDDPLQNLRQMILPAVILGTALSAITMRLTRTMMLEVLRQDYIR
                     TAWAKGLPEKLVVVRHALRNALIPVVTLIGLQAPILIGGAVIVEQIFVIPGMGLLLLD
                     SISQRDYPIITGVSLVIGFSVMLINLAVDLSYGLLDPKVRYR"
     gene            4756..5652
                     /locus_tag="VN968_27865"
     CDS             4756..5652
                     /locus_tag="VN968_27865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL33085.1"
                     /translation="MTLVSDIPVVPASRERSGVIHFLVRLFREKPLGAAGGVIFILFL
                     LCGIFADFLAPYGMNQISPRNRLLPPSWAYPFGTDNLGRDMLSRCLYGAQISVIIAFT
                     AATLATVVSVVIGILTGYLSGKVDLVTQRFVDAWMSFPDLIILLVVVSVLGPGLPQII
                     FTLGFLLGIAGSRIIRSAVVSVRENMYVHAAQSIGASTQRILWRHILPNIMPPIIVLL
                     TTRVGTVILAESSLSFLGLGVPPPTPTWGGMLSGSGRTFMFQGPWLALAPGLCLTIVV
                     YATNMFGDALRDLLDPRMRGSR"
     gene            5691..7397
                     /locus_tag="VN968_27870"
     CDS             5691..7397
                     /locus_tag="VN968_27870"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL33086.1"
                     /translation="MFVTRAARWLAATCFAAAVSLAAPALAQTEQPPPKSGGSLEIAT
                     VFYTLSALSWDLADWNWKQNHDAGQIYEQLFAADLTKARRLGGKHPFYAEAWIPSDAM
                     RGELAESWKWQDPLRLEVTLRKGVMFPEKSGVMAARELVADDVVFSFDRQNKSSKKIS
                     GYFDHVEKVEATGKHTVLFTFKEYNAEWDYRFGWGYYSGIMPKEVADAGAGNWKNVNG
                     TGPFQLADFVQGNSNTYVKNPIYWDTEKIGGVETKLPLVDKIVFRTIKDEATALTALR
                     TGKLDIMENIRWSAVEELKKNAPQLQWSKWLSMLGQFIALRVDTKPFDDIRVRRALNF
                     AVNKQEIVKEYYGGNAELFAYPQHPDFVGYFEPLEAMPDSVKELFTYNPEKAKKLLAE
                     AGVPKGFTFKVQVCTCATDHMDLLPLLASYFEQVGVKIEIQPMEYGAYLSAMTTKTNT
                     PGYMMNNGHTNPTTSIRKSFVTGQVWNPSQWTDREYDKKMEAVYLERDEGKRQALLKD
                     MTREILDKAPYVWLPTPYIFTAWWPWVKNYGGELRAGASRPGPIYARIWVDQDMKKKL
                     GH"
     gene            7394..8413
                     /locus_tag="VN968_27875"
     CDS             7394..8413
                     /locus_tag="VN968_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL33087.1"
                     /translation="MNAPLTPQPYSMERAAQTVPLLSVRDLVTRFRIGRGTVTAVDHV
                     SFDVGAGETLAIVGESGSGKSVTALSILRLIPDPPGVIEGGQILFDGRDLLKLSDAEI
                     RNVRGNRIAMIFQEPMSSLNPVLTIGMQVGEPINQHRRTPWAAALGMARDLLGRVRMP
                     DPASRISAYPHQFSGGMRQRAMIAMALACEPQLIIADEPTTALDVTVQAQILDLLKDL
                     AQSARSALILITHDLGVVARYADRVVVMYAGRVVESAPARELYARPRHPYTRGLMASV
                     PRLDGDTGKRLVPIEGQPPNLAALPAGCAFAPRCQHAIDACRQTRPPLHDVGSQHQVA
                     CILES"
     gene            8417..9406
                     /locus_tag="VN968_27880"
     CDS             8417..9406
                     /locus_tag="VN968_27880"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HXL33088.1"
                     /translation="MPELLLRVDDLKVHFPVKTGAMFRRKVGAVYAVDGVSLTLARGE
                     TLGLVGESGCGKSTTGLAIMRMLAPTAGRIEFEGQDITCYGKKQMRPLRQRMQMVYQD
                     PYGSLNPRMKVRDIIGEPLVVHGLADDSGAYQDKIASLMRTVGLLPDMADRYPHEFSG
                     GQRQRIGIARALALEPSLIVCDEPVSALDVSIQAQVVNVFVELQQRLGLAYLFIAHDL
                     AVVRHVSDRIAVMYLGRIVEIAKRDTLYRSPQHPYTQALLAAVPVADPVVEASRRRVI
                     VSGEVPSATNPPSGCRFHTRCPHAFARCRAEVPQLRDLGDGQAVACHLQEASA"
     gene            9403..10689
                     /locus_tag="VN968_27885"
     CDS             9403..10689
                     /locus_tag="VN968_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXL33089.1"
                     /translation="MTLAKISRSTSRQNGKPAQTHRFIAKRAAILDAAARQFNKYGLK
                     GATLADVAGSVDLLTNSVTYYYRRKEDLVAACLLRAIETMQGLAVEALTASSPEARIR
                     ALISGQAALLADIAAGRRDDLVNFNDIRTLRPPLVNDVFAAYTEMYRSVRRLLPKEGT
                     DRQARNARAHLLLSLVSWIRKWIGRFEPDDYLHAAGRMGDVVIGGLAREQGQWVERPV
                     PELGWPQPATGERAVPEAFLRAATALVNDQGYRGASVEQIAARLNLTKGSFYHHYSSK
                     EELVAACFERTFDVIRHTQMLAMERMDSGWDRLSAAARVLVRFQISEQGPLVRTTAWN
                     ALPPELRTDSQRRFDRLTEKFSIFIVEGMLEGTIPPLDSGIAAQQVAGMINAASELER
                     WVPNIVHENAVRLFAQPLFEGILSSGAAGAAPTSRS"
     gene            10722..12158
                     /locus_tag="VN968_27890"
     CDS             10722..12158
                     /locus_tag="VN968_27890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXL33090.1"
                     /translation="MSGNRCLQRRAQKHALDRTIISSAVREMRRGYRFCQTAAFYRRK
                     RPVNLVPKFLGKHRDCILRPCKANRVADLSVTLETIEALMGQRRRTIAFPAALERQFE
                     EDTQERRCARLRVGLLVSAVLYNFFLIADWLLVPDVFRVATLLHLGLVTPWMLFAAWA
                     MTRRPRPFVRECVAASVPLFICLQIDYGFAATTSESAAHYQYVIIPTLLYANVSLHRL
                     AFRFARAVTAAVVICHAAVVLSASYIAGPVAAMILVQIAICAYITLVANYTMERDLRR
                     AYLFSLRDRLRHSQSDAASKRDALTGLANRHHLDIELRRLWAAGGQPPTMVSVILMDI
                     DHFKRLNDRYGHGAGDLCLKRMASILIAELGPASEGAVRYGGEEFLVVLPAVQLVDAM
                     RIAERIRRSVEIATIPNEGPGLLGIVTASFGVAWASVGELTAAELIAIADTALYAAKR
                     KGRNQVWPPLAAPSGASNIEQISFRDRA"
     gene            complement(12405..13376)
                     /locus_tag="VN968_27895"
     CDS             complement(12405..13376)
                     /locus_tag="VN968_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL33091.1"
                     /translation="MKFVKTILLLLLLSPTLPAFGQEYPSRPITMVVPFSAGGPGDVI
                     ARLLGSSMSITLKQAVIIENVVGAGGTIGTNRVAKAAPDGYTLLLMHVGQATAPALYA
                     KLPFDPVGDFAPIGLVTDVPMILVARSNFPAKDLKELVAYVRAQGDKVTYGNVGLGSA
                     SHLCGLMFMSAIEAKLTPIYYKGGGPALNDIIAGHIDVYCDPATGPTPYIQAGTIPGY
                     AITSKKRVPALPNVPTSAEAGVPGFDVTTWYGMYAPRNTPKPIVDALVGALQKALKDP
                     ALVSRFAELSMTPVEEEQATPAALERLLKTEIEKWDGIIKNAGITPQ"
     gene            complement(13426..14142)
                     /locus_tag="VN968_27900"
     CDS             complement(13426..14142)
                     /locus_tag="VN968_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXL33092.1"
                     /translation="MDKRAELQSTLRIEDVPTVRSRVAQKLREAIMSGTLKPGQRLVE
                     RELCEMTGVSRPSIREALRLLEADGLVTTVPHRGPVVSTISLDEARQLYAARAVLEGF
                     AGRECARLRDPAVVRRIGDALTRLKAAFAEADLMTALEAKTEFYAALIGGCQNTFVER
                     MLRPLHDRITLLRITSMSQPKRINKSLREVTAIWRAIQNGDPELAERCCVDHVKAAAV
                     AALSMIERSSAPKEKAAAHE"
     gene            complement(14221..14934)
                     /locus_tag="VN968_27905"
     CDS             complement(14221..14934)
                     /locus_tag="VN968_27905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL33093.1"
                     /translation="MVTGAAGGMGRAITEALLQSGRRVVLVDRDPESLRQFAAKMGDS
                     TFAIQLDITDAKSVDRLPDLIPNAFKPIDILINNAGHDIGGRTRFDIGPADDWSSIIQ
                     TNLIGLMRVTRSILPDMVRRNSGHIVNISSINAVRIIPDMAAYSTSKAGVHMFTETLR
                     GELAETAIRVTEMQPGLTRTGIILTRYRGDRKKEKDYFGQFKMALDPADIARSILFAL
                     DQPPHVQIAEMMILPVNRY"
     gene            complement(14995..15768)
                     /locus_tag="VN968_27910"
     CDS             complement(14995..15768)
                     /locus_tag="VN968_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL33094.1"
                     /translation="MLKDKWALVTGATAGLGLAVAESLAGAGANIVLHDLVEPRETRD
                     RLRSRFDVDAVSVAADLSQRSSIETMMAELLGRLPAIDILVNNAVVRHFAPVEAFAPE
                     RWEEALAVNLSAPFHLIRLALPAMRKRGWGRIINMASIYSTRAVTDRIDYVTTKTAIL
                     GMTRAVAIETTTSGITCNALCPGTLPTPAIQEKIASIAAGEGRTIDEATRDYLAARQP
                     SGRFVAMEGVGAMVVFLCSLAAQDITGATLPIDGGWSIA"
     gene            complement(15786..16169)
                     /gene="pcaC"
                     /locus_tag="VN968_27915"
     CDS             complement(15786..16169)
                     /gene="pcaC"
                     /locus_tag="VN968_27915"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HXL33095.1"
                     /translation="MDKATYDRGLKIRKSVLGDEFVDKAIASADDFNRPMQDLTTEYC
                     WGYVWGRDGLSHKTRSFLNLAMLCALNRPHELKTHVRGALTNGATQEEIREVFMQVAI
                     YCGVPAGVDAFRNAREVFAELDQKK"
     gene            complement(16204..>16356)
                     /locus_tag="VN968_27920"
     CDS             complement(16204..>16356)
                     /locus_tag="VN968_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HXL33096.1"
                     /translation="SKYYPLGEIKFDEKGRRVGAGMTIVQWQSGVPVTVFPPQLALSQ
                     PFWPKN"
BASE COUNT         2983 a         5004 c         5121 g         3248 t
ORIGIN      
        1 cccgatgcac aaaccatgga cgacctgatc catgacaaga tcgcgccgca cgattggtca
       61 tcccattccg ggagctcaag ctcggtcttt gtcaacgcct cgacatgggc gctcatgctg
      121 actggcgagg tactcgacga cagcgacagc ggcatcgaag gtaccctgcg cggcgtgata
      181 aggcgactgg gagagccggt gatccgcaaa gccatgtcgg cagcgatgcg ggaaatgggc
      241 gagcagttcg tgctcggccg taccatcgac gaggcgatgc gcgccgggcg tgatgtcacg
      301 cgaaaaggct atctctattc ctttgacatg ctcggcgagg cggcgcggac cgagcgcgac
      361 gcgctgcgct accatcgcgc ttatgccgac gcgatcgctt cgatcggggc tgctgctgat
      421 aatgtcgaca gcaacatgaa tcacggaatc tccgtgaagc tatccgcgct gcatcctcgt
      481 tacgaggtgg gccagaagga cacgatgctt ccggtcatcg tccagcgcgt gctgtcgctc
      541 gcgctcgctg cgcgtgacgc gcgcataggc cttaacatcg atgcggagga atccgaccgg
      601 ctcgaccttt cccttggcat catcgagcag gtcctgtcgg attctcggct tgccggctgg
      661 aagggaatgg gggtcgtggt tcaggcgtat ggaccgcgcg cggcttttgt catcgactgg
      721 ctctacgcgc ttgccgaacg cctcgaccgc cggatcatgg ttcgcctcgt caagggcgcc
      781 tattgggaca ccgagatcaa gcgcgctcaa gtgctggggc tcgacggcta cccggtgttc
      841 acgcgtaagc cgaacaccga cgtctcctat attgcctgcg cgaaaaagct gcttggcagg
      901 accggccgca tctatccgca gttcgccacg cacaacgcgc acaccgttgc agctattctg
      961 gcgatggcgg gagatagaga gagctttgaa ttccagcggc tgcacggcat gggcgaagcg
     1021 ctgcacgagg cggcaagagc gaccgagggc acacgctgcc gaatctatgc gccggtgggc
     1081 gcgcattccg aactgctcgc ctatctcgtt cgccgcctgc tcgagaatgg cgcgaactcc
     1141 tccttcgtgc accagatcgt caacaagacg attgaagcgg aagatatcgt gcatgatcca
     1201 ttcgagagcg tcatgacgca gggcccggcc gccaatccgg cgatcccccc tccggcgcgc
     1261 atttttggag ccggacgccg caactccagg ggattcgacc tcgccgatcc cgtcacgctc
     1321 gcgcgaatcg atgccgaaac gaagccgttc gcggcgccgc atctttggaa cgcaggcccg
     1381 atcgtcgctg ccagtttcct tggcggcagg gcacgcagcg tgctcaatcc cgcccggccc
     1441 gacgagcgcg ttggcgaagt tgtcgacgcg ccagcggccg atgtggccag agcggtcgat
     1501 gccggggtca gggcacggac cggctgggag cgaacgtcgg tcggcatgcg cgccgacatg
     1561 ctggtgaagg cggcggatct atacgagcaa aacgcggcag agtttttcgc gctgtgcgct
     1621 cgcgaggccg gcaagactct tggcgatgcc gtatcggaag tgcgcgaggc ggtcgatttc
     1681 ctgcgttact atgccgggca ggcggccaac gccgagcacg atacaggcgc gcgcggcctg
     1741 atcgtctgca tctcgccgtg gaattttccg ctggcgatct tcaccggtca gatcgcagcg
     1801 gcccttgtca ccggcaatgc ggtcgtcgct aaaccggccg agcagacgcc gctgatcgct
     1861 gcgcgcgccg tcgagttgct gcaccaggcg gggattccaa aggacgtgct ccagttgctg
     1921 accggcgatg ggtcggtcgg cgcggcgctg acggccgatc cccggatcga cggcgtctgc
     1981 ttcaccggct ccatggaggt ggcgcgcttg atcgaacagc agatcgcggc gacggctgag
     2041 ccagacgcga tgctgatcgc cgagaccggc ggactcaacg ccatgatcgt cgattcaact
     2101 gctctgcccg aacagtcggt gcgtgacatc cttgcctcgg ctttccagag cgccggccag
     2161 cgctgctcgg cgctgcgaat cctctacgtc caaaaggatg tggaacaaaa gacgctggag
     2221 atgctgaagg gggcgctcgc catgctgagg atcggcgatc cctggctgat ctcgaccgat
     2281 gtcggtccgg tcatcgacga cgaggcgcgc ggagcactca aggcctattg cgcggagatg
     2341 gagaaaaagg gtcgcctgat cgccaaactc gatgcgccgc gagagggctg ctttgtcggg
     2401 ccgcacattt ttcgcgtttc cggcattacc gagatggaac atgagatgtt cggcccgatc
     2461 ctgcatgtcg ccacctttga ggctgacatg atcgacagcg tcatcgcgga gatcaatgcg
     2521 aaacagtatg gcctgacctt cggactgcac acgcgcatcg aggcgcgcgt aaagcacctt
     2581 gtcgaaagta tccatgcagg caacatctac gttaaccgca accagatcgg tgccgtggtg
     2641 ggttcacagc cgtttggggg tgaggggctg tcaggcaccg gtcccaaggc aggtggaccg
     2701 caatatttgc gccgtttccg caaaggtctg acgacggcgg tcaacgccgc cgaagccccc
     2761 gtcgtgtcag cgtctacgct cgccgaacac atgcccgatg cgacgctcgg cggctggtcg
     2821 acaaggcccg atcgcctcgc ggtgctgaga aagcacctgc gtggaaaggg cgccgaagcc
     2881 atcaccgcag cagcggcaat cgacttcggt ccggtggacc tgccgggacc gaccggcgaa
     2941 gccaacacgc tgctgcgcgc gccacgcggc cgcgtcttgt gcctgggact ggatgaggca
     3001 gccgttctgg ctcaatccgt ccaggctctc gcggccggca atgcggtgct ggcggtcgcg
     3061 ccgcgcgcgc aagcatgcct gttgccgctt ttggacaagg gcctgccaat ccgcgccatc
     3121 aacggcacgg tggatgccgg cgatctggtt caactcagcg tcgaggtggt cgcgatctcg
     3181 gccgacgtcg acacgctgcg cgccatacgc cgtgcgctgg cacgacgttc cggtccgatt
     3241 gtgccattgg tgtcggaagt gatcagccca aatgcttacg tgcacgagcg cgccgtgtgt
     3301 gtcgacacga cggcagccgg cggcaatgcc accctgttgg cggccgcgga gcccatcaac
     3361 gtctagccaa acatgaccca tagggcccac tcggaagtct ggttcacact ttcacttacg
     3421 caattggcgc cgctgtcgtg gaactgccga gccgatgagg tggcaggagg aagaagctcg
     3481 gcctcgttcc gcgctggcca agaacgcgta gcttcgccca gtttcatgaa ttgagtatgg
     3541 aacaaaacgt cctctttcat ccaaagcgaa cgggcccggt tggttgatac ctcttgcaag
     3601 cgagaggtgg aatgctatgc tcatgcagtt cccgctcgca ggccatgttt cttctcgaaa
     3661 acagggtacg gcgagcctgc agacaagacg accggttgaa gtcgtcagca acacaaggag
     3721 gaggacgcct tgaagccctg ggcagtggtt ttcgagcttc cggcgcccgc gcgccccacg
     3781 aaaattcgcg ggtagcgtca tgcgcagcta cgtcgtgcgc cgcttgctag cgctggtacc
     3841 gacgttgctg tttgccagcc tgattgtttt cgtcatcgtg cggctggtgc cgggtagcat
     3901 catcgacatg atgctctccc agaacgatgt cggtgccgac aagctgtcgc gcgaccaact
     3961 gatccaaacc ctgggtctcg accgaccgat gtgggagcaa tatttccgtt ggatcggcag
     4021 gatccttctg cacggcgatc ttggccgctc gctctggcag aatactccgg tggccgagct
     4081 tctgcgggcg cgcctgccaa tcactttcca actcggcctg atggcgctga tcgtagccct
     4141 gctaattgcc attccgattg gaatctactc ggccatccgg caggacactg ccggcgatta
     4201 cgtcacacgc tcgttctcga tcctgatgct tgccgtgccg agcttctgga tgggcacgat
     4261 ggtcatggtc tttccgtcga tctggtgggg ctggtcgccg gaagtgaagt ttgtcgcgtt
     4321 cactgacgac ccgttgcaaa acctgcggca gatgatcctc cctgccgtca tcctcggcac
     4381 ggcgctctcg gcgatcacca tgcgcctgac gcgcaccatg atgctggagg tgctgcggca
     4441 ggactacatc cgcaccgcct gggcaaaggg tctcccggaa aaactcgtgg tggtccgcca
     4501 tgcgctgcgc aacgcgctga tcccggtggt cacgctgatc ggcctacagg caccgatcct
     4561 gatcggcggc gccgtaatcg tcgagcagat cttcgtcatt cccggcatgg ggctgttgct
     4621 gctcgattcc atcagccagc gcgactaccc gatcatcacg ggtgtgagcc tggtgattgg
     4681 tttcagcgtg atgctgatca atctcgcggt ggatttgagt tacggcctgc tcgatcccaa
     4741 ggtgagatat cgctgatgac gctggttagc gatattccgg tcgtcccggc cagcagagag
     4801 cgcagcggcg ttatccattt tctggttcgc ctgttccggg agaagccgct cggggccgcc
     4861 ggcggcgtga tcttcattct tttccttctg tgcggaatat tcgcggattt tctcgcgcct
     4921 tacggcatga accagatcag cccgcgcaac cgtctgctgc cgccctcatg ggcctatccg
     4981 ttcggaaccg acaacctcgg ccgcgacatg ctgtcgcgct gcctttatgg cgcacagata
     5041 tcggtgatca tcgcgtttac ggccgcgacg ttggcgacag tcgtctctgt cgtcatcggc
     5101 attctgaccg gttatctcag cggcaaggtc gatctcgtca cgcagcgctt tgtcgatgcc
     5161 tggatgagct tcccggatct catcatactg ctggttgtcg tttcggttct cggtccgggc
     5221 ctgccgcaga tcatcttcac gctcgggttt ctgttgggca ttgccggctc gcgcatcatc
     5281 cgctccgccg tagtgtcggt acgcgagaat atgtacgtgc atgccgcgca atcgattggc
     5341 gcctcgacgc agcgcatcct gtggcggcac attctgccga acatcatgcc gccgatcatc
     5401 gtcctgctta cgacacgggt cggtaccgtc atcctcgccg agtcgagcct ctcgtttctc
     5461 ggcctcggag tgccaccgcc gaccccgacc tggggcggaa tgctgtcggg atcaggtcgt
     5521 actttcatgt tccagggacc atggctcgcg ttggcgccgg gcctgtgcct gacgatcgtc
     5581 gtttatgcga ccaacatgtt cggagatgcc ttacgtgacc tgctcgatcc gcgcatgcgc
     5641 ggttcgagat gaatttgctt ggataaatta atagaacgaa ggaggaagtc atgttcgtta
     5701 ctcgtgctgc acgctggctt gccgccacct gtttcgctgc cgcggtgtcg ctggcagccc
     5761 cggctctggc gcaaaccgag cagccgccac ctaaatcagg cggctcgctt gaaatcgcca
     5821 ccgtcttcta cacactctcg gcgctgtcct gggatctcgc ggactggaac tggaagcaga
     5881 accacgacgc cggccagatt tacgagcaac tgttcgcggc cgatttgacc aaggcccgac
     5941 ggctcggcgg caagcatcca ttctacgcag aagcctggat tccgtcggac gcgatgcgtg
     6001 gcgaacttgc cgagagctgg aaatggcagg acccgttgcg ccttgaagtc accttgcgca
     6061 agggcgtgat gttcccggaa aagtccggag tcatggcggc gcgcgaactc gttgccgatg
     6121 acgtcgtgtt ctccttcgac cgccagaaca agagctccaa gaagatttcc ggttacttcg
     6181 accacgtcga gaaggtcgag gcgaccggca agcacacggt gttgttcacc ttcaaggaat
     6241 acaacgccga atgggactat cgtttcggct gggggtacta ctccggcatc atgccgaaag
     6301 aggtcgcgga cgccggcgcc ggtaactgga agaatgtcaa cggcaccggg ccgtttcagc
     6361 ttgccgattt cgtgcagggc aactcgaaca cctacgtcaa gaacccgatc tactgggata
     6421 ccgagaagat cggcggcgtc gagaccaagc tcccgctcgt ggacaaaatc gtttttcgga
     6481 ccatcaagga cgaggcgacg gcgctgacgg cattgcgcac cggtaagctt gatataatgg
     6541 agaacattcg ctggtccgcg gtcgaagagt tgaagaagaa cgcgccgcaa ctgcagtggt
     6601 cgaaatggct ttcgatgctc ggccagttta tcgcgctccg tgtcgatacc aagccgttcg
     6661 acgacattcg agttcggcgc gcactcaact tcgcggtcaa caagcaggag atcgtgaagg
     6721 aatattacgg gggtaacgcc gaactgtttg cctatccaca gcaccccgac ttcgtgggct
     6781 acttcgagcc gctcgaggcg atgccggact ccgtcaagga gctgttcacg tacaatcccg
     6841 agaaggcgaa gaagctgctg gccgaagccg gcgtgccgaa gggcttcaca ttcaaggtac
     6901 aggtgtgcac ctgcgcgacg gatcacatgg acttgctgcc gctgctggcc tcgtatttcg
     6961 agcaggtcgg cgtcaagatc gagatccagc caatggaata cggcgcgtat ctctcggcga
     7021 tgacaaccaa gaccaatacg ccgggataca tgatgaacaa tggacacacc aacccgacga
     7081 cgtccatccg caagagcttc gtcaccgggc aggtctggaa tccatcgcag tggacggatc
     7141 gcgaatacga caagaaaatg gaggccgtat atcttgagcg cgatgaaggc aaacgtcagg
     7201 ctctgctgaa ggacatgaca cgggagatcc tggacaaggc accctatgtc tggcttccaa
     7261 cgccgtacat cttcacagcg tggtggccct gggtaaagaa ctatggcggc gagctgcgcg
     7321 cgggcgcgtc ccggcccggg ccgatctatg cacgtatctg ggtcgaccag gatatgaaga
     7381 agaagctggg ccattgaacg cgcccctgac gcctcagcct tacagcatgg agcgcgccgc
     7441 gcagacggtg ccgctgctca gcgtccgcga cctcgtgacg agatttcgga tcgggcgcgg
     7501 cacggtcacg gccgtcgatc acgtctcgtt cgacgtcggc gccggagaaa ccctggcgat
     7561 cgtcggcgag tcgggttcgg gcaagagcgt gacggcgcta tcgatcctgc gcttgatccc
     7621 ggatccgccc ggcgtgatcg aaggtggaca gatcctgttc gacggtcggg accttctgaa
     7681 attatctgac gccgagatca ggaacgtgcg cggtaaccgc atcgccatga tcttccagga
     7741 gccgatgagt tcgctcaacc cggtgctgac gatcgggatg caggtgggcg agccaatcaa
     7801 ccagcaccgc cggacgccgt gggcggcggc gcttggtatg gcacgcgatc tgctcgggcg
     7861 ggtgaggatg ccggatcctg cttcgcggat cagcgcctat ccgcaccagt tctcgggcgg
     7921 gatgcgtcag cgtgcgatga tcgcgatggc gctcgcctgc gagccgcaac tgatcatcgc
     7981 cgacgagccg accacggcgc ttgacgtcac cgttcaggcg cagatccttg atctgctcaa
     8041 ggacctcgcg cagagcgcac ggtcggcgct gatcctgatc acgcatgatc tcggcgtcgt
     8101 cgcgcgatat gccgaccgtg tcgtcgtgat gtatgcgggc agggtcgtcg agtcggcgcc
     8161 ggcgcgggag ctatacgcgc gtccgcgtca tccctatacc cgcggcctga tggcctcggt
     8221 gccgcgcctc gacggggaca caggcaagag gctggtgcca atcgaggggc agccgccaaa
     8281 ccttgcagcg ctgccggccg gctgcgcctt cgcgccgcgc tgccagcatg cgatcgacgc
     8341 ctgccggcaa acacgtccgc ccttgcacga cgtcggttca cagcatcagg tcgcctgcat
     8401 tttggagagt tgatcgatgc ccgagctgct gctgcgtgtg gacgacctga aggtccattt
     8461 ccccgtgaag accggcgcaa tgtttcgcag gaaggtcgga gcggtctacg cggtcgacgg
     8521 cgtgtcgctg acgcttgcgc gcggcgagac gctcggtctt gttggcgaaa gcggatgcgg
     8581 aaagtcgacc accggtcttg ccatcatgcg gatgcttgcg cccaccgccg gccgcatcga
     8641 gttcgaagga caggacatca cttgctatgg caagaagcag atgcggccgc tgcgccagcg
     8701 catgcagatg gtttaccagg acccgtacgg ttcgctcaat ccgcgcatga aggtgcgcga
     8761 catcatcggc gagccgctgg ttgtgcacgg actagccgac gacagcggcg cttatcagga
     8821 caagatcgcc tcgttgatgc ggaccgtcgg cctgctgcct gacatggccg atcgctatcc
     8881 gcacgaattc tccggcggtc agcgacagcg gatcggcatc gcgcgcgcgc tggcgcttga
     8941 gcccagtctc attgtctgcg acgaacctgt ctccgcgctc gacgtgtcga ttcaggcgca
     9001 ggtagtcaac gtctttgttg aactccagca gcggctcggg ctagcctatc tgttcatcgc
     9061 gcatgatctc gcggtggtgc gtcacgtaag cgaccggata gcggtaatgt atctcggccg
     9121 tatcgtcgaa atagccaagc gggacacgct ctacaggtcg cctcagcatc cctacaccca
     9181 ggcgctgctc gcggcggtgc cggtcgccga tccggtcgtc gaagccagcc gtcgccgcgt
     9241 gatcgtatcc ggagaggtgc cgagcgcgac aaacccgccg tcggggtgcc gtttccatac
     9301 ccgttgcccg cacgctttcg cgcgctgccg ggccgaggtc ccgcaactgc gtgacctcgg
     9361 cgacggccag gcggtcgcct gtcaccttca ggaagcgagc gcatgacgct tgcgaagatt
     9421 tcgcgaagta catcgcgtca gaacggcaaa cccgcgcaga cgcaccggtt cattgccaag
     9481 cgcgcggcga tcctcgacgc ggccgcgcgc cagttcaaca agtatgggct gaagggcgcg
     9541 acgctcgccg atgtcgcggg cagtgtcgat ctgctgacca acagcgtcac ctattactat
     9601 cggcgcaagg aggatctggt agccgcctgc cttctgcgcg cgatcgagac gatgcagggc
     9661 cttgcggtgg aggcgctcac agcctccagc ccggaggcgc gcatccgcgc gctcattagc
     9721 ggccaggccg cgctgttggc cgatatcgct gcaggcaggc gcgacgacct cgtcaatttc
     9781 aacgacatcc ggactctgcg cccgccgctg gtcaatgacg tgttcgccgc ctatacggaa
     9841 atgtaccgca gcgtccgccg cctgctgccc aaggaaggga ccgaccgcca agcgcgcaac
     9901 gcgcgagcgc atctgctgtt gtcgctggtt agctggatac gcaaatggat cgggcgtttc
     9961 gagccggacg actatctcca tgccgccggt cgcatgggcg acgtcgtcat tggcgggctg
    10021 gcgcgcgagc agggccaatg ggtggaacgt ccagttccgg aactgggctg gccgcagcct
    10081 gctacgggcg agcgggctgt tcccgaggca tttctgcggg cggcgaccgc gcttgtgaac
    10141 gaccaaggct atcgcggcgc atcggtcgag cagatcgcag cgcggttgaa tctcaccaaa
    10201 ggatcattct accaccatta ttcgagcaag gaggagttgg tcgctgcctg cttcgagcga
    10261 acctttgacg tgatccgtca cacgcagatg ctcgccatgg agcggatgga ttctgggtgg
    10321 gaccggctgt cggcggcggc gcgcgtgctg gtgcggttcc agatttcgga gcagggaccg
    10381 ctggtgcgca cgacggcgtg gaacgcgctg ccgccagaat tgcgaacgga ttcgcagcgc
    10441 cgattcgatc gtctcaccga aaagttcagc atcttcatcg tcgagggaat gctcgaagga
    10501 acaatcccgc cactcgactc cgggatcgcc gcacagcagg tcgcgggcat gatcaatgcg
    10561 gcgtcagaac tcgagcgctg ggtgccgaac atcgtgcacg agaatgcggt gcggttgttc
    10621 gcgcagccgc tattcgaagg aatcttgtcg tctggcgcgg ccggcgcagc gcccacctcc
    10681 aggtcatgaa ctcgcaagcg agcatcgaga caagtcctgc ggtgtccggc aatcgctgtt
    10741 tgcagcgccg tgcgcagaag cacgccttgg atcggacgat aatatcttcg gctgttcgcg
    10801 aaatgcgtcg cggttaccgt ttctgtcaga cggccgcatt ttatcgacgt aagcgaccgg
    10861 ttaacctcgt tccgaaattc cttggaaagc atcgggactg catactgaga ccctgcaaag
    10921 caaacagggt tgccgatttg tcagtcacac tcgaaacaat cgaagctctc atggggcagc
    10981 ggaggcgcac gatcgcattc ccggccgcat tggaacgaca gttcgaggaa gatacgcagg
    11041 agcggcggtg cgcgcggctt agagtaggtc tccttgtttc tgccgtcctc tacaatttct
    11101 tcttgatcgc cgattggctg ctcgtccccg acgtatttcg cgttgctacg ttgcttcatc
    11161 tcggcctggt cactccatgg atgctctttg cggcctgggc gatgaccagg cgcccacgtc
    11221 ccttcgttcg cgaatgcgtg gcggccagcg tgccgctgtt catttgtctg cagattgact
    11281 acggctttgc ggccacgacg agcgaaagcg ccgcacacta ccagtacgtt attattccga
    11341 cgctgcttta tgccaacgta tcgctgcacc ggctggcgtt tcgttttgca cgcgccgtga
    11401 ccgcggctgt cgtgatttgc catgccgcag tcgtcctgtc ggcgagctac atcgcgggtc
    11461 cggttgcggc tatgatcctg gtgcagattg cgatctgcgc gtacatcacg ctggttgcca
    11521 actacacgat ggagcgcgat ttgcggcgcg cctatctatt ttccctgcgg gatcgccttc
    11581 gccattcgca gtccgatgca gcatcgaagc gcgatgccct caccggtctg gcgaaccgcc
    11641 atcacctgga catcgagctg cgccggctgt gggcggcggg cggccaacca ccaacaatgg
    11701 tgtcggttat cctgatggac atcgaccatt tcaagcggct caacgataga tatggccatg
    11761 gtgcgggcga tctctgcctg aagcggatgg cttcgatcct gatcgccgag ctcgggccgg
    11821 cgtcggaagg tgcggtccgt tacggcggcg aggaatttct ggtggtgctg cccgcggtgc
    11881 agctggtcga tgcgatgcgg atcgccgaga ggattcgacg gtcggtcgaa atcgcgacga
    11941 ttccgaacga agggcccggg ctactcggga tcgtcacagc gagttttggc gtcgcgtggg
    12001 cctccgtcgg tgaactcact gcggcggagc tgattgccat cgcggatacg gctctttatg
    12061 cggcgaagcg caagggccgg aaccaggtgt ggccgccact tgcggcgccg agcggcgcct
    12121 cgaacatcga gcagatatcg tttcgggacc gcgcttgaat tcccgccgct ccgagaccaa
    12181 tcgtcctcgc tccgcaagct gtcgcccatt ccaacgggag cgtcttcaaa gatgcagtcc
    12241 gaaatcgagt gtccagcggg gccggtacgg caaatgctgg cggcgccgta aggagccaaa
    12301 tcactttcgg agatcaagga cagcaccaag gggtatcgtc tctacacggt cgacggttct
    12361 ggcgaagggt tggcgcagac ctttgccttg gcgacttgcc agccttattg cggggtaatt
    12421 cccgcattct tgataatccc atcccacttc tcgatttctg tcttgagcag gcgttcgagt
    12481 gcagcgggcg tcgcttgctc ctcctcgacc ggcgtcatgc tgagctcggc gaagcggctt
    12541 accagggccg gatccttgag cgccttttgc agggcgccga cgagcgcatc cacgattggt
    12601 ttcggcgtgt tgcgcggcgc atacattccg taccaggtcg tgacgtcaaa tcccggtacg
    12661 cccgcttccg ccgaggtcgg cacgttcggc aaggccggta cacgtttctt gctggtaatc
    12721 gcgtagcccg ggatggtgcc ggcctggata tagggcgtcg gtccggttgc cggatcgcaa
    12781 tagacgtcaa tgtgaccggc gataatatcg ttcagcgccg gtccgccgcc cttgtaatag
    12841 atcggcgtga gtttggcctc gatcgcactc atgaacatca gcccgcaaag atgtgaagcg
    12901 gagcccaggc ccacattgcc ataggtgacc ttgtcccctt gggcgcgcac ataggcgacc
    12961 agttccttca ggtccttggc cggaaagttt gagcgcgcca ccaggatcat cggtacgtcg
    13021 gttacgaggc cgatcggcgc gaaatcgccg accggatcga acggcaactt cgcatagagc
    13081 gccggcgcgg tggcctggcc aacatgcatc agcagcaggg tgtagccgtc gggggcggcc
    13141 ttggcgaccc gattggtgcc gatggtgcca ccggcgccaa cgacattttc gatgattacg
    13201 gcttgcttca gcgttatgct catcgagctt ccgagcaggc gcgcgatcac atcgcccggc
    13261 ccaccggccg agaatggaac caccatggtg atcggccggc tcggatattc ctgtccgaac
    13321 gccggcagtg ttggcgacag cagcagcagc aacagaattg ttttgacgaa cttcattgca
    13381 gtttcctgtc tgccttgcgg gcctttcgaa agcagcgagg actggctatt catgcgcggc
    13441 cgctttttcc ttcggcgcgg atgagcgttc gatcatgctg agcgcggcca ccgcagccgc
    13501 cttcacatga tcgacgcagc accgctcggc gagttcgggg tcgccattct ggatcgcgcg
    13561 ccagatcgcg gtgacctcgc gaagactctt gttgatccgt tttggctgcg acatcgaggt
    13621 aattcgcaat aacgtgatcc ggtcgtgcag cggccgaagc attcgctcga cgaaggtatt
    13681 ctgacagcca ccaataagcg ccgcatagaa ttccgttttg gcttcaaggg ccgtcatcag
    13741 gtcagcttcg gcgaacgcag ctttcaaccg ggtgagagcg tccccgatcc ttcgcacgac
    13801 agcggggtcg cgcagacggg cgcattcacg gccggcgaat ccttcgagca cggcacgtgc
    13861 cgcgtaaagt tgccttgctt catcaagact gatggtgctg acgacaggac cgcgatgggg
    13921 gaccgtcgtg acgagaccgt cggcttccag cagccgaagt gcctcgcgaa tggacggccg
    13981 actgacaccc gtcatctcgc aaagctcgcg ttccaccagc cgctggcccg gcttcaaggt
    14041 cccggacatg atcgcttcgc gtaacttttg cgcgaccctc gaccggacgg ttggaacgtc
    14101 ctcaatccga agtgtggatt gcaattcagc gcgtttgtcc attgtctatc gccttcgttc
    14161 ggtctgtcgc aatgacacgt gcaggtgtcg ccgctgccgc cgaccgctat cggcactgct
    14221 tcaatagcgg tttacgggta gaatcatcat ctccgcaatc tgaacatggg gtggctggtc
    14281 cagcgcaaac aagatcgatc gtgcgatatc cgccggatcg agcgccatct tgaactgccc
    14341 gaagtagtct ttttccttct tcctgtcgcc gcggtagcgg gtgaggatga taccggttcg
    14401 cgtgaggccg ggctgcatct ccgttaccct gatcgccgtc tcggcaagtt cccctctgag
    14461 cgtttccgta aacatatgca cgcccgcctt gctcgtgctg taggcagcca tgtccggaat
    14521 gatacgtacg gcgttgatcg agctgatgtt gacaatgtga cccgagttgc gtctgaccat
    14581 gtcgggcagg atgctgcgtg tcacgcgcat caagccgatc aggttggtct ggatgatgct
    14641 ggaccaatcg tctgccggcc cgatatcgaa gcgcgtccgg ccgccgatgt catgtccggc
    14701 gttgttgatg aggatgtcga tcggcttgaa agcattcgga ataagatcgg gcaggcggtc
    14761 cacgcttttg gcgtcggtaa tatcgagctg gatcgcgaac gtgctgtcgc ccattttggc
    14821 ggcaaactga cgaagggatt ccgggtcgcg gtcgaccaat acgacccggc gtccgctttg
    14881 cagcagcgcc tcggtgatgg cgcggcccat gccgccagcc gcaccggtca ccaccgcgtt
    14941 tcgcaccaat gtttcagcca ttggatatct ccgttgcgga tcgatggttt gcgctcatgc
    15001 gatggaccag cctccatcga tcggcagggt cgcacctgtg atgtcctgcg cggccaggct
    15061 gcagagaaag acaaccatcg caccgacgcc ttccatcgca acaaatcgcc cgctcggctg
    15121 gcgtgccgcc aggtagtcgc gcgtcgcctc gtcgatggtg cggccttcgc cggctgcgat
    15181 cgatgcaatt ttctcctgaa tggccggcgt tggcagcgtg cccgggcaga gggcgttaca
    15241 ggtaattccg ctcgtggtcg tctcgatcgc gaccgcccgg gtcatgccaa ggatggcggt
    15301 cttggtcgtc acataatcga tgcggtcggt gacggcgcgg gtcgaataga tcgatgccat
    15361 gttgatgatg cggccccagc cgcgtttcct catggccggc aaggcaaggc gaatgagatg
    15421 aaacggagcc gacagattga cggccagcgc ttcctcccat cgttccggcg cgaacgcttc
    15481 gaccggcgca aagtgccgga ccaccgcgtt attcaccagg atatcgatcg cggggaggcg
    15541 gccgagcagc tcagccatca tggtttcaat ggatgagcgt tgcgacaggt cggcggctac
    15601 gctcaccgca tcgacgtcaa agcgggaccg aagacgatcc cttgtctctc tcggctcgac
    15661 caaatcgtgc aggacaatat tggctcccgc gccggcaagg ctttccgcca cggccagccc
    15721 aaggcctgcc gttgctccgg tcacgagtgc ccatttgtcc ttcagcatcg gatcgggctc
    15781 cctgcttact tcttttgatc gagctcggcg aatacttccc gtgcattgcg gaaagcatcg
    15841 acgccggccg gcacgccgca atagatcgcg acctgcatga acacctcgcg tatttcctcc
    15901 tgcgtggcgc cgttggtcag cgcgccccga acatgggtct tgagttcgtg cgggcggttg
    15961 agcgcgcaca gcatcgcgag gttgagaaaa ctgcgggtct tgtgcgaaag gccgtcgcgg
    16021 ccccagacat agccccagca atattccgtc gtgagatcct gcattggacg attgaagtcg
    16081 tcggccgacg cgatggcctt gtcgacgaat tcgtcgccaa ggacgctttt gcggatcttg
    16141 aggcctcgat cgtatgtcgc cttgtccatg atgatttcca atgtttgcgg ttgcagggag
    16201 gcgctagttc ttgggccaga acggctgaga cagcgccagt tgcggcggga ataccgtgac
    16261 cggaacgccg gattgccact gcacgatcgt catgccggcg ccgacacgcc ggcccttttc
    16321 gtcgaacttg atctcgccga gcggataata ttttga
//
LOCUS       DATNOI010000700         4037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_104724,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000700 DATNOI010000000
VERSION     DATNOI010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104724"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2310
                     /gene="ppc"
                     /locus_tag="VN968_27925"
     CDS             <1..2310
                     /gene="ppc"
                     /locus_tag="VN968_27925"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HXL33097.1"
                     /translation="HPTEVRRKSTMDREMEIAALLDRRERVQLTPEEFEADAEQLRRA
                     VLTLWQTNLLRRTKLTVLDEVANGLSFYDYTFLQEVPRLHCALEDRLNHVGDGPRGEL
                     ASFLRMGSWIGGDRDGNPFVTAEVMRGTLRLQSSRIMRFYLEELHVLGAELSLAAHLA
                     DVSKDLRALAGRSPDTSPHRSGELYRLAVSGVYARLTATALKLDVETIRQPVGAAAPY
                     DSVKELKADLDVLHRSLVSNNSGVIARGRLRHLRRAVDCFGFHLANLDMRQNSAVHER
                     TVAELLDAATPGMSYLALSEEARIALLSNELRNARPLTSAFVKYSEETLGELAVFHGA
                     AEAHSKYGASVIPQCIISMCKGVSDMLEVALLLKEAGLVDPSGRCVINIVPLFETIED
                     LQASAAIMDRMLSLHDYRKLVDSRGSVQEVMLGYSDSNKDGGFVTSGWELYKAEIGLI
                     ETFERHHVRLRLFHGRGGSVGRGGGPSYDAIIAQPGGAVNGQIRITEQGEIISSKYSN
                     AEVGRNNLEILAAATLEASLLQPRQSAPSGEYLKAMEQFSALAFKAYRGLVYETEGFA
                     DYFWASTVITEISSLNIGSRPASRKKTHEIEDLRAIPWVFSWAQCRLMLPGWYGFGSA
                     VEAWVAEHPDKGMPFLMELYREWPFFRTLLSNMDMVLAKSSIAIASRYAELVPDATLR
                     ENIFGRIRREWHLSIETLLDIMGQERLLQGNPLLERSIRNRFPYLDPLNHVQVELLKE
                     HRAQNPDEQVLRGIQITINGISAGLRNSG"
     gene            complement(2390..3268)
                     /locus_tag="VN968_27930"
     CDS             complement(2390..3268)
                     /locus_tag="VN968_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXL33098.1"
                     /translation="MAVERVATGFRWAEGPVYFPAGRYVLFSDIPNNRIMRFSEDDGH
                     LSIFRQPSMNSNGNTIDREGRLITCEHSGRRVTRTELDGSITIIADKYNGKKLNSPND
                     AVVASDGSIWFTDPAYGIGGFYEGLKAEPEQEKHNVYRVDPKSGDVKVVVDDFVEPNG
                     IAFSPDEKKLYVLDTGFTDGPDNPSHIRVFDVDGGAGKVSNSKVFAEMPKPSITDGVR
                     CDTEGRVWCSVGWGDPKEDGVRCYTREGELLGKIHIPETVANLCFGGQQRNRLYICGS
                     TSLYAVYTSVQGALKP"
     gene            complement(3834..>4037)
                     /locus_tag="VN968_27935"
     CDS             complement(3834..>4037)
                     /locus_tag="VN968_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HXL33099.1"
                     /translation="AAKDVTGQIFAVRMNEIFLMSQSRPLRSIHRAEGWTPQTLAEHG
                     IPALKSSFYKLDRSADIFNWDAI"
BASE COUNT          738 a         1193 c         1307 g          799 t
ORIGIN      
        1 caccccacgg aagtccgccg caagagcacg atggaccgcg agatggagat cgccgcgctg
       61 ttggaccggc gcgagcgggt ccagctcacg ccggaggaat ttgaggcgga cgccgagcag
      121 ttgcgccgcg cggtgctgac gctgtggcag accaatctgt tgcgccgtac caagctcacc
      181 gtgctcgacg aggtcgccaa cggcctctct ttttatgatt ataccttcct gcaagaggtg
      241 ccgcggctgc attgtgcgct ggaggaccgg ttgaaccatg tgggtgatgg tccgcgcgga
      301 gagctggcgt cgttcctgcg gatgggaagc tggattggcg gcgaccgcga tggcaacccg
      361 ttcgtgacgg cggaagtgat gcgcggaacg ttgcggctgc agagcagccg cataatgcgc
      421 ttttatctcg aggagttgca cgtcctcggc gccgaactgt cgctcgcggc gcatcttgcc
      481 gatgtctcga aggatttgcg tgcgctcgcc gggcgctcac ccgacacttc gccgcatcga
      541 agcggcgagc tttaccggct ggcagtatcc ggtgtttatg cgcggctcac ggcaacggcg
      601 ttgaaactgg atgtggaaac catccggcag ccggtcggag cagcggctcc ctatgacagt
      661 gtgaaggagc tcaaggccga cctcgatgtg ctgcatcgat cgctggtttc aaacaattcg
      721 ggtgtgatcg cgcgcgggcg gctgcggcac ttgcgccggg cggtggattg cttcgggttt
      781 cacctggcca acctcgacat gcggcaaaac tccgccgtgc acgaacgcac ggtcgcggaa
      841 ttgctcgacg ccgcgacccc cggcatgtcc tatctggcgc tgagcgaaga ggcgcggatc
      901 gcgctgctct cgaacgagtt gcgcaacgcg cggccgctga cctcggcatt cgtcaaatac
      961 tccgaagaga ccctgggcga actcgcggta tttcatggcg ctgccgaagc gcattcgaaa
     1021 tacggcgctt ccgtgatccc gcaatgcatc atctcgatgt gcaagggcgt gtccgatatg
     1081 ctggaggtcg cgctgctcct gaaggaggcc ggcctcgtcg acccctccgg tcgctgcgtg
     1141 atcaacatcg tgccgctgtt cgagaccatc gaggatttgc aggcgtccgc cgccatcatg
     1201 gaccggatgc tgtcgctgca tgattatcgc aagctggtgg acagccgtgg cagcgttcag
     1261 gaagtgatgc tcggctattc cgacagcaac aaggatggcg gttttgtcac gtcgggctgg
     1321 gaactctaca aggccgagat cggcctgatc gagacattcg aacgccacca cgtccgcttg
     1381 aggctgtttc acggccgtgg cggctcggtc ggccgcggcg gcgggccgag ctatgacgcc
     1441 attatcgcgc agccgggcgg cgcggtgaac ggccagatca ggatcaccga gcagggtgaa
     1501 atcatctcca gcaaatattc caacgccgaa gtcggccgca acaatctgga aattctcgcc
     1561 gcggccacgc tggaggccag cctgctgcag ccgcggcaaa gcgcgccatc gggcgaatac
     1621 ctcaaggcga tggagcaatt ttcggcgctg gccttcaagg cgtatcgcgg gctggtctac
     1681 gaaaccgaag gttttgcgga ttatttctgg gcctccaccg tcatcaccga aatttcctcg
     1741 ctgaacatcg gcagccgtcc ggcgtcgcgc aagaaaaccc acgagatcga ggaccttcgc
     1801 gccattccct gggtgttcag ctgggcgcaa tgccggctga tgctgccggg ctggtacggt
     1861 tttgggagcg cggtggaagc ctgggtcgcc gagcatcctg ataagggcat gccgttcctg
     1921 atggagctct atcgcgagtg gccgttcttc cgcacgctat tgtcgaacat ggacatggta
     1981 ttggcaaaaa gctcgatcgc gatcgcgtcg cgctatgccg aattggtgcc ggatgcgact
     2041 ttgcgcgaaa atatctttgg acgcatccgc cgcgaatggc acctctcgat cgaaacgctg
     2101 ttggacatca tggggcagga gcgcttgctg cagggcaacc cgctgctgga acgctcgatc
     2161 cgcaaccgct ttccttatct cgacccgctg aaccacgtcc aggtggagtt gctgaaggag
     2221 caccgcgcgc agaatcccga cgagcaggtg ctgcgcggga ttcagatcac catcaacggg
     2281 atttcggcgg ggctgcggaa cagcgggtga ggaaggacgc gaatagcgaa tggcgagtag
     2341 cgaatggaaa atccctattc gctactcgcc actccctatt cgccgggcgt cagggcttca
     2401 aagcgccctg cacactcgtg tagactgcat agagcgatgt cgagccgcag atgtaaagtc
     2461 tgtttcgctg ctgaccgccg aagcacaggt tggcgacagt ttccggtatg tggatcttgc
     2521 cgagcaactc gccttcccgc gtgtagcagc gcacgccgtc ctctttcgga tcaccccaac
     2581 cgacggagca ccagacgcgc ccttcggtgt cacagcgcac cccgtcggta atgctgggct
     2641 tgggcatttc cgcaaacact ttgctgttcg acacctttcc ggccccccca tccacgtcga
     2701 acacgcggat atgcgacggg ttgtccggcc cgtccgtgaa gccggtgtcg agcacataga
     2761 gcttcttctc gtcgggcgag aaggcaatgc cgttgggctc cacgaagtca tcgacgacaa
     2821 ccttgacgtc accggatttc ggatccactc ggtagacgtt atgcttttcc tgctcaggct
     2881 cggctttgag gccctcataa aagccgccga tgccgtaggc tggatcggtg aaccagatcg
     2941 atccatcgga agcgacaacc gcgtcgttcg gcgagttcag cttcttgcca ttgtacttgt
     3001 cggcaatgat cgtgatcgag ccgtcgagtt cggttcgggt gacccgtcgg ccgctatgtt
     3061 cgcaagtgat caggcgtcct tcccgatcaa tggtgttgcc gttcgagttc atcgacggct
     3121 ggcggaatat gctcaggtga ccgtcatcct ccgagaagcg catgatgcgg ttgttgggaa
     3181 tgtcgctgaa cagcacgtag cggccggctg ggaagtagac cgggccctcg gcccagcgaa
     3241 agccggttgc aacgcgctcg accgccatcg tgccggcgaa ggctgggaag ccgctcggcc
     3301 cgaacgaaac cttgggcttg gccgactcca ggtgagaatc cggatagcgg cccccgggta
     3361 gcggtcccag gggtagcgga tcggttgaca gggtcgtggc acccgtttgg ccgaatggcg
     3421 tcacggtcgc cgcagaggcc gctttcatgg ttacggccgt tgcggccagc gccgccgcgc
     3481 ccctgatcag tttgcggcgg ttgaaaccgc cggcggcggc tccggcagat tggtcgggtg
     3541 catttatttg gtttgtcatg gtgtcctccc aggatttttt tgtttgggag ggcaatcgtg
     3601 agccgacatt ccggcataaa gcaggcggat gttggggaag caaccaggga tggacgggag
     3661 gagtgcgagg ggcgagccga gccgatagtg caatgcgtca tcccggcgtt cgccggggat
     3721 ccctacgtcg tgccgtcgcg atttagcgca ggaggtcgcg accttccgca tccatgagtg
     3781 ttagtggtcc ggcgctcgcc gggacaactc gtggagcggt gtggcgtctg cttctagatc
     3841 gcgtcccagt taaaaatatc cgccgaacgg tcgagcttgt agaacgacga cttcaacgcg
     3901 gggatgccgt gctcggcgag ggtctgcggc gtccagcctt cggcgcgatg gatcgagcgt
     3961 aacgggcgcg actggctcat caggaaaatt tcgttcatcc gcaccgcaaa gatctgcccg
     4021 gtgacatcct tggcggc
//
LOCUS       DATNOI010000701        17145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_473973,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000701 DATNOI010000000
VERSION     DATNOI010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 17145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17145
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473973"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="VN968_27940"
     CDS             <1..174
                     /locus_tag="VN968_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HXL33100.1"
                     /translation="VAGDGGDITFRGFKDGIVYLNMKGSCSGCPSSTATLQHGIQNLL
                     KHFVPDVVEVRPV"
     gene            313..1011
                     /gene="tsaB"
                     /locus_tag="VN968_27945"
     CDS             313..1011
                     /gene="tsaB"
                     /locus_tag="VN968_27945"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXL33101.1"
                     /translation="MLILAIDTALDACAAGVLDTGAGELIAQESQSMKRGHAEALMPL
                     IARVIKESGIAFASLDRIAVTTGPGSFTGLRVGLSAARGIALAANKPVVGLTTLTAYA
                     APVVSQNAEQPVITAIDARHDHVYFQVVSGNGSSLIRPRVAPIEEALGASRFGAPHLV
                     GNAAKILADRWPADAVPPFTIDAQPAPDIAWVAWLGAAVSPITAPARPFYLRAPDAKL
                     PKDRLQKASQPSAS"
     gene            1008..1493
                     /gene="rimI"
                     /locus_tag="VN968_27950"
     CDS             1008..1493
                     /gene="rimI"
                     /locus_tag="VN968_27950"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HXL33102.1"
                     /translation="MMAWLSELWSGGAAAIEPATLRDAARLAQLHGASFHRGWGEGEF
                     EAMLTERNTLVHRLRIGRKIIGFAVSRMAADEAEILSIAVAFSHRGRGLSSNLLLTHL
                     GHLAGRGVRTVFLEVEENNQPARRLYERAGFAVAGRRERYYRQPNGEQLNALMMRRDL
                     S"
     gene            1649..2098
                     /locus_tag="VN968_27955"
     CDS             1649..2098
                     /locus_tag="VN968_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HXL33103.1"
                     /translation="MTVLKSPSTLKATGIEARCAATGMRMTEQRRVIARVLAEALDHP
                     DVEELYRRCVAVDDKISISTVYRTVKLFEDAGIIERHDFREGRARYEQMRDSHHDHLI
                     NLRDGKVIEFTSEEIEKLQAEIARKLGYKLVDHRLELYCVPLDDDKS"
     gene            2347..3786
                     /gene="miaB"
                     /locus_tag="VN968_27960"
     CDS             2347..3786
                     /gene="miaB"
                     /locus_tag="VN968_27960"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HXL33104.1"
                     /translation="MKAPRKLHIKSYGCQMNVYDAQRMVDTLAPEGFVETSSVDDADL
                     VILNTCHIREKASEKVYSELGRLRIAKDEAARNGRQMSIAVAGCVAQAEGQEIIRRAP
                     VVDVVVGPQSYHHLPQLLARARSQGRALETEFPVQDKFGFLAPPSADAIRARGISAFV
                     TVQEGCDKFCTFCVVPYTRGAEVSRPVAKIVDDAQRLADNGVREITLIGQNVNAYHGE
                     GPDGRTWSLGALLHRLAEIPGIVRLRYSTSHPRDVDDSLIAAHRDLPALMPFVHLPVQ
                     SGSDRVLAAMNRRHTADDYRRVIDRFRAVRQDIAFSSDFIVGFPGETEEDFASTLALV
                     TQIGYAGAYSFKYSPRPGTPAADMRETVSAAAMDERLVRLQGLIDSQQSAFNVAAIGN
                     TVDVLFERAARNAGQIVGRTAYLQPAHVMASPDIIGQVLPVRIESLERYSLLGELAAS
                     PDAPRLRHDFSRIANSPHIANSSHVTTGA"
     gene            3791..4846
                     /locus_tag="VN968_27965"
     CDS             3791..4846
                     /locus_tag="VN968_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HXL33105.1"
                     /translation="MPKSASDSPSLAPSRKLDRDMQIPPETQVVIDFDDNRAASALVG
                     PYGQNLALVERRLGVVVDSRGNHITIAGSRDGCDAARRVLETLYAQATQGHDLAQGDV
                     EGAIRAVIAQGSLFEFDAKNVRSAFETINLRKRPVRARTAAQDSYIRALKRHEMVFGI
                     GPAGTGKTWLAVAHAAQLFERKEVDRIILSRPAVEAGERLGFLPGDLREKVDPYLRPI
                     YDALYDLMDSRIVERALQAGEIEIAPLAFMRGRTLTNAVIILDEAQNTTSMQMKMFLT
                     RLGENSRMIITGDPSQVDLPNGQTSGLAEAARLLDGVEGIAQVKFTAEDVIRHELVAR
                     IVAAYEGLPQRPATSKP"
     gene            4909..5430
                     /gene="ybeY"
                     /locus_tag="VN968_27970"
     CDS             4909..5430
                     /gene="ybeY"
                     /locus_tag="VN968_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXL33106.1"
                     /translation="MALSALPMTEVLVVADCWQTEPDAEAVIHRAIATAAEIVNAGPD
                     KAVGEAELAVMLTDDAGIRTLNNNWRGIDKPTNVLSFPALPPTGKSGPDDAPPMLGDI
                     AIAYETTRKQADDEQKPFDHHLSHLAVHGFLHLIGYDHESDDDADAMETLEQEILAQL
                     GIPDPYADRERMG"
     gene            5432..6547
                     /locus_tag="VN968_27975"
     CDS             5432..6547
                     /locus_tag="VN968_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXL33107.1"
                     /translation="MADSEPTHDNPRNTRNLPAVVQEGEVLRPAADNWLIRAIRTVFG
                     WKPGSVRDDLQVVLDASTPDEVGFSAIERTMLRNILGLHERRIADVMIHRADIVAVKR
                     DIPLGELMSLFESAAHSRLVVYNETLDDPEGIVHIRDLLVFMTAKARAHAGKATRKKP
                     LPAGLDLRAVDLALPLADANIIRKLLFVPPSMRAIDLLAQMQASRIHLALVVDEYGGT
                     DGLVSIEDIVEQIVGEIDDEHDSDEPPAIVRQPDNSFIADARASLDDVRSVIGEDFVT
                     GEAGEEVETLGGYLVTHVGRLPVRGEVISGPGNFEIEVLDADPRRVKRLRIATRKERP
                     AVRTPREIRRRESTPEAGPQQANDNTSPPSGDGAGSQ"
     gene            6544..8157
                     /gene="lnt"
                     /locus_tag="VN968_27980"
     CDS             6544..8157
                     /gene="lnt"
                     /locus_tag="VN968_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HXL33108.1"
                     /translation="MNSAKKLRSIGLAIILSWGWKRAAIALLAGALSALAMAPFNAWP
                     VLFATFPVVVWLIDGAGAGAWRGVPAAAMAGWWFGLGYFVPGLYWIGNAFLVDASTFA
                     WLMPFAVLGLPAYLALFTAFGFALARLIWTRDASRVIALAASLTLGEWLRGHVLTGFP
                     WNTLGYALTEPLALAQTASLIGLWGLTFLAVAIFASPAVLIDGSSRGRRPWIAPAAAL
                     LLLVAMGIFGAVRLTLQPTVTVANIKLRIMQPNLQQDVRFNYAAKADVMQKYLTLSDR
                     ASGPQSTGVRDAGILIWPESAFPFFLTREADAMAQISDLLPKGTILITGSVRAPDLPP
                     GTQITRAYNSIYAIDHDGRVLSIYDKLHLVPFGEYLPYQDWMEKLGFVQLTKVQGGFI
                     PGVRRRVIAFPNAPPALPLICYEAIFPVIVGASDERPGWIVNLTNDGWFGISTGPYQH
                     LQQARLRAIEQGLPVVRAANTGISAVIDPLGRMIARLGLGIEGVLDSSLPAAIPPTIY
                     ARTGDIPAAAVVAAALIFIIRRRVAKRTF"
     gene            8405..8821
                     /locus_tag="VN968_27985"
     CDS             8405..8821
                     /locus_tag="VN968_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL33109.1"
                     /translation="MSTKAPNPVDKYVGSRVRMRRIMLGMSQEKLGEALGLTFQQVQK
                     YEKGTNRVGASRLQQISEILQVPVSFLFEGGPSGATNAGGFSEGTSPSYVSDFLATSE
                     GLALTRAFTRISDAKLRRSIVEMVEQIAIREGPDER"
     gene            9099..10253
                     /locus_tag="VN968_27990"
     CDS             9099..10253
                     /locus_tag="VN968_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXL33110.1"
                     /translation="MTTAKSSNPFNSSNRFDANTILDGIRRWVEIETPTEAPGQVNRL
                     ATLVADGYRDLPVTIERIAGHSGCGDHILARSSWGQDAPGILVLSHLDTVHPMGFIER
                     LPFRVKGDSAFGPGIHDMKGGAYLAYHAFRQLCESDERSPLGVTQLYVSDEEIGSPTS
                     RALIEAEGRKAKYVLVTEPARDGGKIVTGRKGVARFEIFVHGVPAHAGSRPEDGRSAI
                     RELATIIQTLEALTDVRRGVTVNVGVVRGGTRPNVIAEDAYAEVDLRVRTPADADEFV
                     PKILNLKSRSEGVSVKVVGELNRPPYEKGNAGAALFEHAKTLAAEIGFDLADTFTGGG
                     SDGNFTAPHTATLDGLGVDGQGAHTHYEQMYISSIEPRARLLHRLYQTLR"
     gene            10250..11005
                     /locus_tag="VN968_27995"
     CDS             10250..11005
                     /locus_tag="VN968_27995"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HXL33111.1"
                     /translation="MSIAARDPTHRDTNSNGSPDDGTAAPAHGSFFGRRKGHRLRLHQ
                     ADLIEHLLPRLSLDIGRPGPADLAGLFDTPVDDLRLEIGFGGGEHLVAEARAFPKTGF
                     IGCEPYVNGMAKILTQIEAHAIGNIRLFAGDAAELLAWVRPRSLARIDLIHPDPWPKR
                     RHWKRRFVQDLTVAAMARVLKPDGEFRFVSDIADYCAWTLAHLTRSPDFVWTAEQASD
                     WRLPWAGYTMTRYGRKAEREGRHAAYLRFRKVG"
     gene            complement(11073..12155)
                     /locus_tag="VN968_28000"
     CDS             complement(11073..12155)
                     /locus_tag="VN968_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HXL33112.1"
                     /translation="MSIARSLIPELDEIVRHGDPRRRAEAARRITELFLRGAATFRSD
                     HVDLFDGVLIDLVPHSELAARADLAERLSVLANAPRALVGQLAREDEIAVAGPLLRRS
                     PVIDEQALIAIARIKGQGHLLAMSERPTLSCDLTDVIIRRGDRAAVRRAAGNAGALFS
                     QAGYSALIKRASQDGVLTLAIGKRDDLSNQQLRDLLAGSIDIVRRRLFEVVKPARQAD
                     IKQAISEISGEAEPVASGRDFAPAQRTILALHRAGELNQSALLGFAKAYKYEESIAAL
                     SAMSAVKIATLDRLIMGDRHDPILIVGKAVGLEWATVRALILLRLGPNRVPSPADIES
                     ARVNFGRLMPSTAERVVSFWQTRQSA"
     gene            12508..13278
                     /gene="rimP"
                     /locus_tag="VN968_28005"
     CDS             12508..13278
                     /gene="rimP"
                     /locus_tag="VN968_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HXL33113.1"
                     /translation="MTDPTAGSVDTDLLAEPRLVVEPGMAARVSAVAGPVLQGMGYRL
                     VRIKISGEFGCTVQIMAERPDGSMQIDDCEAISRALSPVLDVADPIERAYRLEISSPG
                     IDRPLVRRSDFERYAGHLVKIEMAVAHQGRKRFRGTLGGVEGDAVHLHRDDIRAGEDP
                     DVVLVMEDIADARLVLTDELIAESMRRGKAAERELKQNLGLAPPPPPYARKSDPAKSN
                     KPKPKPKPEKKSAPKNTKEHRLAAERLRRGEFDPTEGD"
     gene            13282..14895
                     /gene="nusA"
                     /locus_tag="VN968_28010"
     CDS             13282..14895
                     /gene="nusA"
                     /locus_tag="VN968_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HXL33114.1"
                     /translation="MAAVSANKLELLQIADAVAREKSIDRGIVIAAMEDAIAKAARAR
                     YGSETDVHAEIDAKKGELRLSRHMLVVDVVENSSNQISLVDAQRANPGAQVGDTIADT
                     LPPLEYGRIAAQSAKQVIVQKVREAERDRQYQEFKDRIGDIVNGIVKRVEYGSVIVDL
                     GRGEAIVRRDEMLPREVFRNGDRVRAYIFDVRRETRGPQIFLSRTHPQFMAKLFAQEV
                     PEIYDGIVEIKAVARDPGSRAKIGVISRDSSVDPVGACVGMRGSRVQAVVNELQGEKI
                     DIIPWSPDIATFVVNALAPAEVAKVVIDEERERIEVVVPDTNNQLSLAIGRRGQNVRL
                     ASQLTGWDIDILTEQEESERRQADFENSTRVFMEALNVDEVVGQLLASEGFTSVEELA
                     LVDVKELAGIEGFDDETATELQNRAREYLEQLEGELENKRKELGVDDALKTVPGVTSK
                     MLVKFGENEIKTVEDLAGCATDDLVGWTERKEGGEPTKHPGILDPNETSRDEAENMIM
                     QARVIAGWITEADLVKKTDEAETPEDQPV"
     gene            14969..15676
                     /locus_tag="VN968_28015"
     CDS             14969..15676
                     /locus_tag="VN968_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXL33115.1"
                     /translation="MLAFADPDLDNGPRTDRSATVRMCAVTRQVRPIDELIRFVVSPS
                     GEVMADLKRKLPGRGLWISASRQAVAEAVRRSQFSKGFRRDVKVAPTLPADTESLLVR
                     GVIDALAMAAKAGQVVSGFSKVEGALEQRQVQNSVKALIHAADGAADGIRKLDAVVRQ
                     NTANNGESREFPVVTVLTSEQLDLALGRSNVVHAALLAGPASKTFLSRSHVLVRYRMA
                     DDDKTAGKAAKNSETDR"
     gene            15752..>17145
                     /locus_tag="VN968_28020"
     CDS             15752..>17145
                     /locus_tag="VN968_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HXL33116.1"
                     /translation="MVDTKTPGDKTLSVPTKTLTLKPRVETGTVRQSFSHGRTKQVVV
                     EKRGKRRVGGDAPAAESHAPEPVVAKAAPAKAPLARPAAPPTPQRSNSGVVLRTLTED
                     ERSARASALADAKVRDIEERRLAEEEAKRRNSKEGIEQAEREAAEARRKAEEERHRQD
                     EEAKRKAELEAKKRFGEAEAKAAATVRAAAAVPAARAPGIAADITDEDEGPRQIRRGP
                     GGVVRPAAAPKTTAKPAPAKQRGRLTLVTALNADDVRERSIASFRRRTQRLKGHASNE
                     PKEKLIREVIIPEAINIQELANRMSERAVDVIRLLMKQGAMHKITDVIDADTAQLIAE
                     ELGHTVKRVAAADVEEGLFDIVDDSTDIEPRSPVVTVMGHVDHGKTSLLDALRHANVV
                     SGEAGGITQHIGAYQVISPESGKKITFIDTPGHAAFTAMRARGAKVTDIVILVVAADD
                     GVMPQTIEAINHAKA"
BASE COUNT         3308 a         5461 c         5411 g         2965 t
ORIGIN      
        1 gtcgccggcg acggcggcga catcacgttc cgcggcttca aggacggcat cgtctatctc
       61 aacatgaagg gctcctgttc gggctgcccg tcgtccaccg cgacgctgca gcatggtatt
      121 cagaatttgc tgaagcattt cgtgcccgac gtggtcgaag tccggccggt gtgatcttac
      181 gctgaaagca tcgcttattt ccgcgtcatg cccgggcttg tcccgggcat ccacgttttc
      241 aggcgatagc aaagacgtgg atggccggga caagcccggc catgacggct taaacagtga
      301 tatcatcagc ccatgctgat ccttgccatc gacaccgccc tcgacgcctg cgccgcaggc
      361 gtcctcgaca ccggcgccgg cgagctgatt gcgcaggaat cccagtccat gaagcgcggc
      421 cacgccgaag ccttgatgcc gctgatcgcg cgcgtcatca aagagtccgg catcgcgttc
      481 gcgtccctcg accgcatcgc ggtaaccacc ggccccggca gctttaccgg gctgcgggtc
      541 ggcctttccg ccgcgcgcgg cattgcactc gccgccaaca agcccgtggt cgggctgacc
      601 acgctgacag cctatgccgc gcccgtggtc agccagaacg ccgagcagcc ggtcatcacc
      661 gctatcgatg cacggcatga ccatgtgtat tttcaggtgg ttagcggcaa tggcagttca
      721 ttgatccggc cgcgggtcgc gccgatcgag gaggcgctcg gcgcatcgcg gttcggcgcg
      781 ccgcatctgg tcggcaacgc cgccaaaatt ctcgctgacc gctggccggc ggacgcggtg
      841 ccgcctttca cgatcgatgc gcaaccggcg ccggacattg cctgggtggc gtggctgggt
      901 gccgcggtca gtccgatcac cgcgcctgcc cgcccgtttt atctgcgcgc gcccgacgcc
      961 aaacttccaa aggaccggct gcagaaagcc tcgcagccat cagcgtcatg atggcgtggc
     1021 tctccgaact gtggagcggc ggcgcggcgg cgatcgaacc cgcgaccctg cgtgatgcgg
     1081 ctcgtctggc acaattgcat ggcgcatcgt tccatcgcgg ctggggtgaa ggcgaattcg
     1141 aggctatgct gaccgagcgc aacacgctgg ttcaccgcct gaggatcgga cggaagatta
     1201 tcggctttgc ggtgtcgcgc atggcggcag acgaggccga aatactttcg atcgccgtgg
     1261 cctttagcca tcgcggccgc ggcctgtcca gcaatctgtt gctgactcat ctcggccatc
     1321 tcgccggccg cggcgtgcgt accgtgttcc tcgaggtcga ggaaaacaat cagccggcgc
     1381 gccggctcta tgagcgggcc ggatttgccg tcgccggacg ccgcgaacgc tattaccggc
     1441 agccgaacgg ggaacaattg aacgcgctta tgatgcgccg cgacttgtcg tagcgcccgc
     1501 gcggtggcac aacatgcaca gggcgcgatc agcaaagtgg gatccggttt tgcgtcggag
     1561 cgcgcgctaa actgtaaaag gagcgcatga tctttaccgc caaaccgaat acactttgac
     1621 ggatcatgcg ccgcccaagg caaaccccat gaccgtattg aaatccccgt ccacgctgaa
     1681 ggccaccggc atcgaagcgc gctgcgctgc caccggcatg cgcatgaccg aacagcgccg
     1741 cgtcatcgcg cgggtgctgg cggaggcttt ggatcatccc gacgtcgagg aactgtatcg
     1801 ccgctgcgtc gcggtcgacg acaagatctc gatctcgacg gtgtatcgaa ccgtcaagct
     1861 gttcgaagac gccggcatca tcgagcggca cgactttcgc gagggccgcg cccgctatga
     1921 acagatgcgt gacagtcacc acgaccatct gatcaatctg cgcgacggca aggtgatcga
     1981 attcacctcc gaggagatcg agaagctgca ggctgaaatc gcccgcaagc tcggctacaa
     2041 gctggtcgat caccggctgg aactgtactg cgtcccgctg gacgacgaca aatcgtgatc
     2101 gcggcgcgct ggctctcaat cgccgtcatt ccgggacacg cgtcttcgcg gggacctcag
     2161 atgtgcaatc gcacatcggg gaatcctggg attccggttc gatgcggagc ccgtcatcgg
     2221 gccggccaaa ggccggaccc gttggcatcg ccccggaatg acggtaaaga ctggatttcc
     2281 ccagctttgg gctatatttg agcctggcgc cgcaatggcg caatctcggc ccaacattca
     2341 ggttccatga aggcgccgcg caagctgcat atcaagtcat atggttgtca gatgaatgtc
     2401 tacgatgcgc aacgcatggt ggacacgctg gccccggaag gctttgtcga gacgtcgagt
     2461 gtggatgacg cggatctggt gatcctcaat acctgtcaca tccgcgaaaa ggcctccgag
     2521 aaggtttact cggaactggg gcggctgcgc atcgccaagg acgaggccgc gcgcaatggc
     2581 cgccagatga gcatcgcggt cgccggctgc gtcgcgcagg ccgaaggcca ggagatcatc
     2641 cgccgcgccc cggtggtgga tgtggtggtc ggcccgcaaa gctatcacca tctgccgcaa
     2701 ctgctcgccc gcgccaggag tcagggccgc gcgctggaga ctgaatttcc ggttcaggac
     2761 aagttcggat tcttggcccc gccgagcgcg gatgcgatcc gcgcgcgcgg tatctcggcc
     2821 ttcgtcaccg tgcaggaagg ctgcgataaa ttctgcacgt tctgcgtcgt gccctatacc
     2881 cgcggcgccg aagtgtcgcg ccccgtcgcg aagatcgtcg acgacgcgca aaggctcgcc
     2941 gacaatggcg tgcgcgaaat cactctgatc ggacagaacg tcaatgccta tcacggcgag
     3001 ggaccggacg gccggacctg gtcgctcggc gccttgctgc atcgcctcgc cgagattccc
     3061 ggcatcgttc gcctgcgcta ctcgaccagc catccccgcg atgtcgatga ttcgctgatc
     3121 gcggcgcacc gcgatttgcc ggcgttgatg ccgttcgtgc atcttccggt acaatcgggt
     3181 tccgaccggg ttcttgctgc gatgaaccgc agacataccg cggatgatta ccgccgggtt
     3241 atcgaccgat tccgcgccgt acggcaagat attgcatttt catcggattt tatcgtgggc
     3301 ttccccggcg aaaccgagga agatttcgcc agcaccctcg cgctggtcac acaaatcgga
     3361 tacgctggcg cttattcgtt caaatattcg ccgcggccgg gaacaccggc ggcggacatg
     3421 cgggagacgg tgtcagcggc tgcgatggac gaacgattgg tgcggctcca gggattgatc
     3481 gacagccagc aatcggcctt caatgtggct gcgattggta acaccgtcga tgtgctgttc
     3541 gaacgcgccg cccgcaatgc cggccagatc gtcggccgca ccgcctatct gcagccggcg
     3601 catgtgatgg cctcacccga tatcatcgga caggtattgc cggtgaggat cgaaagcctc
     3661 gagcgctaca gcctgctcgg tgaactcgcc gcatcgcctg acgcgccgcg cttgcggcac
     3721 gatttttctc gaatcgccaa ctctcctcac atcgccaatt cctctcacgt cacaacggga
     3781 gcctgaaccc ttgccaaaaa gcgcatcgga ttcgccttcg ctcgctccca gccgcaaact
     3841 tgaccgcgac atgcaaatcc cgcccgaaac gcaagtcgtg atcgacttcg acgacaatcg
     3901 cgcggcctcg gcgctggtcg gaccctacgg acaaaacctg gcgctggtcg aacgccggct
     3961 cggcgtcgtg gtcgattcgc gcggcaatca catcaccatc gcgggctcgc gcgacggctg
     4021 cgacgccgcg cgccgcgtgc tggagacgct gtacgcccag gcgacacagg ggcacgatct
     4081 ggcgcagggc gatgtcgaag gcgcaatccg cgcggtgatc gcgcagggct cgctgttcga
     4141 gttcgacgcc aagaacgtgc ggtcggcgtt cgagaccatc aatttgcgca agcgtccggt
     4201 gcgggcgcgg acggccgcgc aggattccta tatccgcgcg ctcaagcgcc acgaaatggt
     4261 gttcggcatc ggtcccgccg gtaccggcaa gacctggctc gcggtggcgc acgccgcgca
     4321 attgttcgaa cgcaaggaag tcgatcgcat tatcctgtcg cggccggcgg tcgaggccgg
     4381 cgaacggctc ggcttcctgc ccggcgattt gcgcgagaaa gtcgatccct atctgcggcc
     4441 gatctacgac gcgctgtacg atctgatgga ttcgcggatc gtcgagcgcg cgctgcaggc
     4501 cggcgaaatc gagatcgctc cgcttgcctt catgcgcggc cggactctca ccaacgccgt
     4561 catcattctg gacgaagcgc aaaacaccac ttcgatgcag atgaagatgt tcctgacgcg
     4621 gcttggcgag aacagccgca tgatcatcac cggcgatcct tcccaggtcg acctgccgaa
     4681 cggccagact tcgggtctcg ccgaagccgc aagactgctc gatggcgtcg agggcatcgc
     4741 ccaggtgaag ttcacggccg aagacgtgat ccgccatgaa ctggtggcgc gtatcgttgc
     4801 cgcctatgaa ggattgccgc agcggccggc aaccagcaag ccttgatgcg caaacctgcc
     4861 gcgagcgtgc cgggggcgag gcccccccgc aacagagatt ccggaccgat ggcgctgtct
     4921 gcccttccca tgaccgaagt tctcgtcgtc gccgattgct ggcagaccga gccggacgcc
     4981 gaagcggtca tccatcgcgc catcgcaacc gcggcggaga tcgtcaatgc cgggccggat
     5041 aaagcggttg gcgaagccga actcgcggtc atgctgaccg acgatgccgg aatccgaacc
     5101 ctcaacaaca attggcgtgg catcgacaag ccgaccaacg tgctgtcgtt tccagccctg
     5161 ccgccgaccg gcaagagcgg ccccgacgac gccccgccca tgctcggcga catcgccatc
     5221 gcctatgaaa cgacgcgaaa gcaagccgac gacgagcaaa agccgttcga tcatcacctc
     5281 agccatctgg cggttcatgg cttcctgcac ctgatcggct acgatcacga aagcgacgac
     5341 gacgccgatg cgatggaaac gctcgagcag gagattttgg cgcaactcgg cattcccgat
     5401 ccctatgcgg atcgggaacg gatgggctga gatggcggat tcggaaccga cccacgacaa
     5461 tccgcgcaac acgcgcaacc tgccggcggt ggtacaggaa ggcgaagtgt tgcgtccggc
     5521 cgccgacaac tggctgatcc gcgcgatccg cacggtgttc ggatggaaac ccggctcggt
     5581 ccgcgacgat ctgcaggtcg tgctcgatgc aagcaccccg gacgaggtgg gcttttcggc
     5641 gatcgagcgc accatgctgc gcaatattct cggcctgcac gaacggcgca tcgccgacgt
     5701 gatgatccat cgcgccgaca tcgtcgcggt caagcgcgat attccgctcg gcgagttgat
     5761 gagcctgttc gaaagcgcgg cgcattcgcg cctggtggtc tacaacgaaa ccctcgacga
     5821 tcccgaaggc atcgttcaca tccgcgatct gctggtattc atgaccgcca aggcccgcgc
     5881 ccatgcaggc aaggcgacgc gcaagaagcc tttgcccgcc gggctcgatt tgcgcgcggt
     5941 cgatctcgcg ctgccgcttg cggacgccaa catcatccga aaactgcttt ttgtgccgcc
     6001 ctcgatgcgg gcgattgatc tgttggcaca gatgcaggcc tcgcgcatcc atttggcgct
     6061 ggtggtggat gaatatggcg gaaccgatgg cctggtgtcg atcgaggaca tcgtcgaaca
     6121 gatcgtcggc gagatcgacg acgagcacga cagcgacgag ccgccggcga tcgtgcggca
     6181 gccggataac tcctttatcg ccgacgcccg cgccagcctc gacgatgtcc gctccgtgat
     6241 cggcgaggat ttcgtcaccg gcgaagccgg agaggaagtc gagacgctcg gcggttatct
     6301 cgtgacccat gtcggccgcc tgccggtgcg cggcgaagtc atttctggcc cgggcaactt
     6361 cgaaatcgaa gtgctcgacg ccgatccaag acgggtcaag cgcctgcgga tcgcgacgcg
     6421 caaggaacgc cccgcggtgc gcacgccgcg cgagatccgt cgccgcgagt cgacgccgga
     6481 ggccggtccg caacaggcca atgacaatac gagcccgccg tccggcgacg gagccggctc
     6541 gcagtgaact ccgcaaagaa actccgcagc atcggtctgg cgatcatcct gtcatgggga
     6601 tggaagcgcg ccgcgatcgc gctccttgcg ggggcgttgt cggcgctggc gatggcgccg
     6661 ttcaatgcat ggccggtgct gttcgcaact tttccggtgg tggtctggct gatcgacggc
     6721 gcgggcgccg gagcctggcg cggcgtgccg gcggccgcca tggccggctg gtggttcggg
     6781 ctcggctatt tcgtgccggg actgtactgg atcggcaatg cgtttctggt cgatgcatcc
     6841 accttcgcgt ggttgatgcc gttcgccgtc ctcggcctgc ccgcttatct ggcgctgttc
     6901 accgccttcg gcttcgcgct ggcgcgcctg atctggaccc gggatgcttc gcgggtgatc
     6961 gcgctcgccg ccagcctgac gctaggcgaa tggctgcgcg gtcacgtgct caccggcttt
     7021 ccctggaata cgctcggcta cgccttgacc gagccgctgg cactggcgca gaccgcatcg
     7081 ctgatcgggc tgtggggcct gacatttctt gccgttgcga tcttcgcgag cccggcagtg
     7141 ctgatcgatg gcagttcgcg cgggcgaaga ccctggatcg cgccggcggc agcgctgctg
     7201 ctgttggtcg cgatgggaat tttcggcgcc gtgcgcctga ccctgcagcc caccgtgacg
     7261 gttgcgaaca tcaagctgcg gatcatgcag cccaacctgc agcaggacgt ccgattcaat
     7321 tacgccgcca aggcggacgt gatgcagaaa tatctgacgc tgtccgaccg tgcgtccggg
     7381 ccgcagtcca ccggcgtgcg cgacgccgga attttgatct ggccggaatc ggcgtttccg
     7441 tttttcctga cgcgcgaagc cgatgcgatg gcacaaatat ccgatctgtt gccgaagggc
     7501 accatcctga tcaccggctc ggtgcgcgcg ccggatctgc cgcccggcac ccaaatcaca
     7561 cgcgcctaca attcgatcta cgccatcgac catgatggcc gtgtgctgtc gatctacgac
     7621 aagctacact tggtgccatt cggagaatat ctgccctacc aggactggat ggaaaaactc
     7681 ggcttcgtgc aactcacgaa agttcagggc ggatttattc caggcgtgcg tcgccgcgtg
     7741 atcgcgtttc cgaacgcgcc gcccgcgcta cctctgatct gctatgaagc aatatttccc
     7801 gtaatagttg gggcgagcga cgagcgccca ggctggatcg tcaacctgac caatgatggc
     7861 tggttcggaa tctcaaccgg cccgtatcaa catctgcagc aggcgcgcct gcgcgcgatc
     7921 gagcaggggc tgccggtggt tcgcgcagca aacaccggca tttccgcggt gattgatccg
     7981 ctcggccgga tgatcgcacg gctcggtctc ggcatcgaag gcgtgctgga ttccagtttg
     8041 ccggcggcca ttccgccgac gatttacgca cgcacaggcg atattcccgc agccgccgtc
     8101 gtcgctgccg ccctgatttt catcattcgg cgacgcgtcg cgaagcgaac tttctgacgc
     8161 tcgcaacctc atgtttgaat gagcttgagg gccaaaaaac ggcatgggca aatccattgg
     8221 ttgacagatc ggcggcgcca tttgcattct ccgcccgtcg aggattgatc caatcagtta
     8281 aattgctcaa attggcagca ttcaccaact ctccgagcag gatttgacgg tattggcgcc
     8341 cgaggcatac aacgctttcg ttctgcctca ccaccagcgc gcgtaatccc aaggagtgtt
     8401 ggaaatgtcg actaaagcgc ccaaccctgt tgacaaatat gtcggcagcc gtgtgcgtat
     8461 gcgccgtatc atgctcggta tgagccagga aaaactaggt gaagcgctgg gcctcacatt
     8521 ccagcaggtt cagaaatacg agaaaggcac caaccgggtc ggggcaagtc gccttcagca
     8581 aatatctgaa atacttcaag taccggtgtc gtttttattc gagggcgggc ctagcggcgc
     8641 cacaaacgcc gggggtttca gcgaaggaac gtcgccgtcc tatgtttccg attttctcgc
     8701 gacctccgag ggtttggcgc taacccgcgc gttcacgcgc atctcggacg ccaagcttcg
     8761 gcgcagcatt gtcgagatgg tcgagcagat cgccatccgc gaaggacccg atgagcgcta
     8821 gacgagattc gtgattccgc gaggtcgaaa tggccgcatt gatgcccgag aactgcgaca
     8881 aaattgccgc acccggccac aatctgcggt agacgggcga gtttggaccc cagaacaacc
     8941 gccgggtgcg tcgattccgg gcctgcgcca tccaacccat gtgtcgccgg ctggacactg
     9001 atatgcttaa ccgacgaacc cgcgttcggt gaacgcgtcc acggatgtgc aacggcacat
     9061 cgggaatgaa aggttaaaat tcctctgaag gcggcagcat gacgacggcc aaatcctcca
     9121 accctttcaa ttcctccaac cgtttcgatg ccaacaccat cctcgacggc atccgccgct
     9181 gggtcgaaat cgagacgcct accgaggccc cgggccaggt caaccgactg gcgacgctgg
     9241 tcgccgacgg ctatcgcgat ttgcccgtca ccatcgagcg gatcgcagga cactccggct
     9301 gcggcgatca tatcctggcg cgttcgtcat ggggccagga cgcgccgggg atcctggtgc
     9361 tgagccatct cgacaccgtt catcccatgg gattcatcga acggctgccg ttccgggtaa
     9421 agggcgacag cgctttcggt cccggcatcc acgacatgaa gggcggcgcc tatctcgcct
     9481 atcatgcgtt ccgccagctt tgcgaaagcg acgagcgttc gccgcttggc gtcacccagc
     9541 tctacgtctc ggacgaggag atcggcagcc cgacgtcgcg cgcgctgatc gaggccgagg
     9601 ggcgcaaggc gaaatacgtc ctcgtcaccg agcctgcccg cgacggcgga aaaatcgtca
     9661 ccggccgcaa gggcgtcgcg cgttttgaaa tcttcgtcca cggcgttcct gcccatgccg
     9721 gcagccggcc cgaggatggc cgcagcgcca tacgcgaact cgccaccatc attcaaacgc
     9781 tggaagcctt gaccgacgtc aggcgcggcg taaccgtcaa tgtcggtgtt gtcaggggcg
     9841 gcacccggcc gaacgtcatc gccgaggatg cctatgccga ggtcgatctt cgcgttcgca
     9901 cccccgcgga tgccgacgag ttcgtgccaa aaatcctcaa cctgaaatcg cggtccgaag
     9961 gcgtcagcgt caaggtcgtc ggcgagttga accggccgcc ttacgagaaa ggcaatgccg
    10021 gcgcggcatt gttcgaacac gccaagacgc tcgccgcgga aatcggcttc gatcttgcgg
    10081 ataccttcac cggcggcggc tccgacggca atttcaccgc accgcacacc gcgacgctcg
    10141 acggcctcgg cgtcgatggc cagggcgcac atacccatta cgagcagatg tatatttcgt
    10201 cgatcgagcc gagggcgcgg ctgctgcatc ggctgtatca gacgctgcga tgagcatcgc
    10261 agcccgcgat cccacccatc gcgacacgaa cagcaatgga tcgcccgatg acggcaccgc
    10321 cgctcccgcc cacggttcgt ttttcggacg gcgcaagggc cacaggctgc gtttgcacca
    10381 ggccgacctg atcgaacact tgctgccgcg cctttcgctc gacatcggca ggcccggccc
    10441 cgccgatctc gctggcctgt tcgatacgcc ggtcgatgat ctcaggctcg aaatcggatt
    10501 cggcggcggc gaacatctgg tcgccgaagc ccgcgccttt ccaaagaccg gcttcatcgg
    10561 ctgcgaaccc tatgtcaacg gcatggccaa gatcctgacg cagatcgaag cccatgccat
    10621 cggcaacatc cggctgttcg caggcgacgc cgcggaattg ctggcatggg tgcggccgcg
    10681 ctcgctggcg aggatcgacc tgatccatcc cgatccgtgg ccgaagcggc ggcactggaa
    10741 gcggcgcttc gtgcaggact tgaccgtcgc cgcgatggcg cgcgtgttga agccggacgg
    10801 cgaatttcgc ttcgtcagcg atatcgcaga ctactgcgct tggacgcttg cgcatcttac
    10861 acgctcgccc gatttcgtct ggaccgccga acaggcctcc gactggcgcc tgccctgggc
    10921 cggttacacg atgacgcgat acggacgcaa agccgaacgc gaaggccgcc atgcggctta
    10981 tctgcggttc cggaaggttg gctaactggc gcactcatcg ctgtcattcc ggggcatcgc
    11041 gcagcgatga acccggaatg acgtcctgca actcacgccg attggcgggt ctgccagaag
    11101 ctcaccaccc gctcggccgt cgaaggcatc aggcgtccga aattcacccg cgcgctctcg
    11161 atatccgcgg gcgatggcac ccgattggga cccaaccgaa gcaggatcag ggcgcgcacg
    11221 gtggcccatt cgaggccgac cgctttgccg acgatcagga tcggatcgtg acggtcgccc
    11281 atgatcagac ggtcgagggt tgcgattttc accgccgaca tggcggaaag cgcggcgatc
    11341 gattcctcgt atttgtaagc cttggcaaag ccaagcagcg cgctttggtt cagttcaccg
    11401 gcgcgatgca gcgcgaggat ggtgcgctgc gccggcgcaa agtcgcggcc gctcgcgacc
    11461 ggctcggcct cgccggaaat ctcacttatc gcctgcttga tgtcggcctg ccgcgcaggc
    11521 ttgaccacct cgaacaggcg gcggcgcacg atatcgattg atcccgccag caggtccctc
    11581 aattgctggt tggagagatc gtcgcgcttg ccgatcgcga gcgtcaggac gccgtcctgg
    11641 ctggcgcgct tgatcagcgc cgaatagccg gcttgggaaa aaagcgcccc ggcattgccg
    11701 gcggcgcgcc tgaccgccgc gcggtcgccg cggcgtatga tgacgtcggt gagatcgcaa
    11761 gaaagcgtgg gacgctccga catcgccagc agatggccct ggcccttgat gcgcgcgatc
    11821 gcgatcaagg cctgttcatc gatgaccggc gatcggcgaa gcaagggacc tgcgaccgcg
    11881 atctcgtcct cgcgcgcgag ttgcccgacc agggcgcggg gcgcgttggc gaggacggac
    11941 agccgttcgg ccagatcggc ccgcgcggcg agttcggaat gcggaacgag atcgatcaaa
    12001 acgccgtcaa acaagtcgac gtgatcggac cggaaagttg cggcgccccg caggaacagc
    12061 tcggtgatcc gtcgggcggc ctcggcgcgg cgcctgggat cgccgtgcct gacgatttca
    12121 tcgagctcgg ggatcagcga tctggcaatt gacatcaaac tcactcaaac ccccgcaaac
    12181 cccgagccat ctgcgagttt tcaggcaatt taggtgggct tggtgaagga agggttagat
    12241 ttgaataagg ggccaaacga gccgcaaatt cattcgcccg ggcgtcgcgg gccttgccgg
    12301 atagcgcaga aaccgctata tcagcgctaa cttttggttc tcatacgatc gcgtattgag
    12361 agtgggcccc ttgggacccg ctctttttta ttacccgtac gagcctgacg ctgatgaacg
    12421 gcgccggctc gctcgggtag cccagccgga ccgccctgaa ggcccggctc caacccaatg
    12481 caagttagac caactttgac cctggatatg accgatccaa cagctggttc cgtggatacc
    12541 gatctgcttg ccgagccgcg tctggtggtc gagcccggca tggcggcgcg ggtgtcggcg
    12601 gtcgcagggc cggtgctgca gggaatgggc taccggctgg tgcgcatcaa gatatcggga
    12661 gaattcggct gcaccgtcca gatcatggcg gaacggcccg acggatcgat gcagatcgac
    12721 gattgcgagg cgatttcgag ggcgctgtcg ccggtgctgg acgtcgccga tccgatcgag
    12781 cgggcctacc ggctggagat ttcctcaccg ggaatcgacc gtccgctggt ccgccgttcg
    12841 gatttcgaac gctatgccgg ccatctcgtg aagatcgaaa tggcggtcgc gcatcagggc
    12901 cgcaagcggt tccgcggcac cctcggcggc gtcgagggcg atgccgtgca cttgcatcgc
    12961 gatgacatcc gcgccggcga agatccagat gttgtgctgg tgatggaaga tatcgccgac
    13021 gcgagactgg ttctgaccga cgaactgatc gccgaatcga tgcggcgcgg caaggcggcg
    13081 gaacgagagc tcaagcagaa cctcgggctt gcgccgccgc caccgcccta tgccaggaag
    13141 agcgaccccg ccaagagcaa caagccgaag ccgaaaccaa agccggaaaa aaagagcgcc
    13201 ccgaaaaata ccaaggaaca ccgccttgcc gcagaaagac tgcgtcgagg cgaattcgat
    13261 cccaccgaag gagactgagc catggcagct gtcagcgcca ataaactcga actgctgcag
    13321 atcgcagacg cggttgcgcg ggaaaaatcg atcgaccgcg gcatcgtgat cgcagcgatg
    13381 gaggacgcaa tcgccaaggc cgcgcgggcg cgctacggca gcgagaccga cgttcacgcc
    13441 gagatcgatg ccaaaaaggg cgaattgcgg ctgtcgcgcc acatgctggt ggtcgacgtg
    13501 gtggaaaatt cctcgaacca gatttcactg gttgacgccc agcgcgccaa tccgggcgcc
    13561 caggtcggcg acaccatcgc cgacactttg ccgccgctgg aatatggccg catcgccgcg
    13621 caatcggcca agcaggtgat cgtgcagaag gtgcgcgagg ccgagcgtga ccggcagtat
    13681 caggaattca aggaccgcat cggcgatatc gtcaacggca tcgtcaagcg ggtcgaatat
    13741 ggcagcgtca ttgtcgatct gggccgtggt gaagccatcg ttcgccgcga cgagatgctg
    13801 ccgcgcgagg tattccgcaa cggcgaccgc gtgcgtgcct atattttcga cgtgcgacgc
    13861 gaaaccaggg gaccgcagat ttttctctcc cgcacccatc cgcaattcat ggcgaagctg
    13921 ttcgcgcagg aagtgccgga aatctatgac ggtatcgtcg agatcaaggc ggtcgcccgc
    13981 gatcccgggt cgcgtgcgaa aatcggcgtg atttcaaggg attcgtcggt cgacccggtc
    14041 ggcgcctgcg ttggcatgcg cggctcgcgc gtgcaggccg tggtcaacga gttgcagggc
    14101 gagaagatcg atatcattcc ctggtcgccc gacatcgcga ccttcgtcgt caacgcgctg
    14161 gcgcctgccg aagtcgccaa ggtcgtgata gacgaggaac gcgagcgcat cgaggtcgtg
    14221 gttcccgata ccaacaacca gctgtcgctg gcgatcggcc ggcgcggaca gaacgtgcgt
    14281 ctcgcctcgc aactgaccgg ctgggatatc gacattctga ccgagcagga agagtccgag
    14341 cgccgccagg ccgatttcga gaactcgacg cgggtattca tggaagcgct gaacgtcgac
    14401 gaagtggtcg ggcaattgct ggcgtcggaa ggtttcacct cggtggagga actggcgctg
    14461 gtcgacgtca aggaactggc cggcatcgag gggttcgacg acgaaaccgc gaccgaattg
    14521 cagaaccgcg cccgcgaata cctggaacag ctcgaaggcg aactcgaaaa caaacgtaag
    14581 gaactcggtg tggatgacgc tttgaaaacg gtgcccggcg tgacctcaaa aatgctggtg
    14641 aagtttggtg agaacgagat caagaccgtc gaggatctgg ccggttgcgc caccgacgat
    14701 ctggtcggct ggaccgagcg caaggaaggc ggcgaaccga ccaagcatcc cggcatcctc
    14761 gatcccaacg aaacctcgcg cgacgaggcg gagaacatga tcatgcaggc gcgcgtcatc
    14821 gccggctgga tcaccgaggc cgatctcgtc aagaagaccg acgaggccga gacccctgaa
    14881 gaccagccgg tttgaggcgt gttcctcaac cacgaacacc ggttttgcgc gaccgcaacg
    14941 cgcagatgtt aggagcgatg tcgcaaccat gcttgctttc gccgaccccg atctcgacaa
    15001 cggaccgcgg accgacaggt ctgcgacggt gcggatgtgc gcggtcacgc ggcaggtgcg
    15061 gccgatcgat gagctgatcc ggtttgtggt ttcgccttcg ggcgaggtca tggcggattt
    15121 gaaacgcaag cttcccggcc gcggcctctg gatttcagcg tcccgtcagg cggttgcgga
    15181 agctgtgcgg cgtagccaat tcagcaaagg gttcaggcgc gacgtaaagg tggcgccgac
    15241 gcttccggcg gataccgaaa gcttgctggt gcgcggcgtg atcgacgcgc tcgccatggc
    15301 cgccaaggcc ggccaggtcg tatccggctt cagcaaggtc gaaggcgccc tggaacagcg
    15361 gcaggtccaa aattcggtca aagcccttat ccatgcggcg gacggcgcgg cggatggaat
    15421 caggaaattg gacgcggtgg tccggcaaaa taccgcgaat aatggtgaat cgcgtgaatt
    15481 tccggtggtc acggtgctaa cctcggaaca attggatttg gcactggggc ggtcaaatgt
    15541 ggtacatgct gccctgctcg cgggcccggc gagcaagacg ttcctgtcgc gcagccatgt
    15601 cctggtccga taccggatgg cggatgacga caagacagcc ggcaaagcgg ccaagaattc
    15661 tgagacggac cgctgacgaa gacttgcata agacgacttg cataaaatga ctgtgcgggt
    15721 acgcacaacg caacaagatt aggactgctg tatggttgat acgaaaacgc ctggcgacaa
    15781 gactctcagt gtcccgacca agaccttgac gctcaagccg cgcgtcgaga cgggcaccgt
    15841 tcgccagagc ttcagccacg gccgcaccaa gcaggtggtg gtcgaaaagc gcggcaagcg
    15901 ccgggtcggc ggcgacgccc ccgcggcaga aagccatgcg ccggagccgg ttgtcgccaa
    15961 ggcagcgccg gccaaggcgc cgctcgcccg tcccgccgcg ccaccgactc cgcagcggag
    16021 caattcgggc gtggtgctgc gcaccctgac cgaggacgag cgctcggcgc gcgccagcgc
    16081 gctcgccgac gccaaggtcc gcgacattga agagcgccgt ctcgccgaag aggaagccaa
    16141 gcgccgcaac agcaaggaag gcatcgagca ggccgagcgc gaagccgccg aagcccgccg
    16201 caaggccgaa gaagaacgcc accggcagga cgaggaggcc aagcgcaagg cggagctcga
    16261 agccaagaaa cgctttggcg aagccgaagc caaggccgca gctacggttc gtgccgccgc
    16321 ggcggtgccc gccgcgcgcg ctcccggcat tgccgccgac atcaccgacg aggatgaagg
    16381 cccgcgccag atccgtcgcg gccccggcgg cgtcgttcgt cccgcggctg ccccgaagac
    16441 gaccgcgaag ccggcgccgg cgaagcagcg cgggcgcctg accctggtca ccgcccttaa
    16501 tgccgacgac gtgcgcgagc gctcgatcgc gtcgttccgc cgccgcaccc aacgcctgaa
    16561 ggggcacgcg tcgaacgagc cgaaagagaa gctcattcgc gaagtgatca tcccggaagc
    16621 catcaatatc caggaactcg ccaaccgcat gtccgaacgc gcggtcgacg tgatccggct
    16681 gttgatgaaa cagggcgcga tgcacaagat caccgatgtg atcgatgccg ataccgcgca
    16741 gctgatcgcc gaggagcttg gtcacaccgt caagcgcgtc gccgcggcgg acgtcgagga
    16801 aggcctgttc gatatcgtcg acgattcgac cgatatcgag ccgcgctcgc ccgtggtgac
    16861 cgtgatgggc cacgtcgatc acggcaagac ctcgcttttg gatgcgctgc gccatgccaa
    16921 tgtcgtttcc ggcgaggccg gcggcattac ccagcatatc ggcgcctatc aggtaatctc
    16981 accggaaagc ggcaagaaga tcaccttcat cgacaccccg ggccacgccg ccttcaccgc
    17041 gatgcgcgcg cgcggcgcca aggtgaccga catcgtgatc ctggtggtcg cggcggacga
    17101 tggcgtcatg ccgcagacca tcgaggcgat caatcacgcc aaggc
//
LOCUS       DATNOI010000702         4186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_290323,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000702 DATNOI010000000
VERSION     DATNOI010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290323"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VN968_28025"
     CDS             complement(<1..920)
                     /locus_tag="VN968_28025"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HXL33117.1"
                     /translation="MSLEFEIQPATNPTSENERAARLVDPGFGRIFTDHMAVVRYNQA
                     NGWHGARVESRANFPLDPATAVLHYAQEIFEGLKAYKRDDGGVNLFRPDANARRFRNS
                     AERMAMAALPEPVFIEAIEQLVRIDRAWMPGGEGSLYLRPFMIASEVFLGVKPSAEYI
                     FAVIASPVGSYFKGGPAPVSIWVSENYTRAAIGGTGAVKCGGNYAASLRAQAEAIDHG
                     CDQVVFLDAVERRYIEELGGMNVFFVFDDGSLSTPPLGTILPGITRDSIIALAKDSGT
                     RVREEAYTIEQWRTDAASGKLKEAFACGTAA"
     gene            1150..1290
                     /locus_tag="VN968_28030"
     CDS             1150..1290
                     /locus_tag="VN968_28030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33118.1"
                     /translation="MFAEMPLDNRTVDKFAALAACSAFLRPLETSPRAKRLIVSSRLM
                     VL"
     gene            1314..1868
                     /locus_tag="VN968_28035"
     CDS             1314..1868
                     /locus_tag="VN968_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXL33119.1"
                     /translation="MADINFSRPSPGPDSRPAEGRSHSPRPGDLRWDIIELLFFAYRD
                     FVGDADHELEAFGFGRAHHRVLHFVHRYPGLKVADLLDVLRITKQSLGRVLKQLLDQG
                     YIVQKTGNNDRRQRLLFATAKGEALVAKLAGLQTDRINRALRDIGSSGADTVRQFLRA
                     MIDHDDPDKVLETILRAGNATAKE"
     gene            1865..2593
                     /locus_tag="VN968_28040"
     CDS             1865..2593
                     /locus_tag="VN968_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL33120.1"
                     /translation="MIVPLAATIARTLPQPADDAPHILLVDDDRRIRDLLSRFLSGEG
                     YRVTTAMSASDARAKLLGLHFDLLILDVMMPGETGFDLARFIRSSSSVPIIMLTARHE
                     AEARIEGLQIGADDYVAKPFEPRELVLRIGNILKRTAPPPVTALEQVAFGPYVYHLDR
                     GELRQGDEAVHLTDREREMLRILAVTPGETVPRSALTGEGTVNERAVDVQINRLRRKI
                     ERDPANPLFLQAVRGIGYRLVASP"
     gene            2605..3990
                     /locus_tag="VN968_28045"
     CDS             2605..3990
                     /locus_tag="VN968_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL33121.1"
                     /translation="MSTLDTGLTLIRTAAGRVSAANGWMGNAFKSWMPTGLYARALLI
                     MIVPMVVLQTVVAFVFMERHWNTVTRRLSAAVVQDIASLIDVYRIYPQDKDRAQLRRI
                     GQRLQLVVDFLPVGDLPPPGPKPFFSLLDQTLSVQLGRQIARPFWIDTVGRSNLVEIR
                     IQLDDAVMRIFAQRSSAYASNSEIFIFWMLGTSSILLIVAVLFLRNQIRPILRLADAA
                     ESFGKGREAPNFRPRGAREVRRAAQAFLEMKSRIERSIEQRTAMLAGVSHDLRTILTR
                     FKLELELIGDSPEVDGMRKDVDEMSGMLEDYLAFARGDTGEQAQPTDMALALEELRSD
                     AERNGHAATVAFHGLPVVTVKPASFKRCLANLVSNAARHANAISITGHRDHRYLTVTV
                     DDDGPGIPQEMREEVFKPFLRLDDARNQDEGGTGLGLAIARDIARSHGGDIMLGDSPM
                     GGLRATARIPV"
BASE COUNT          718 a         1372 c         1339 g          757 t
ORIGIN      
        1 gccgccgtgc cgcaggcgaa ggcctctttc agctttccgc tggcggcatc agtgcgccac
       61 tgctcgatgg tgtaggcctc ctcgcgcacg cgcgtgccgg aatccttcgc gagcgcgatg
      121 atcgaatcgc gggtgatgcc aggcaggatc gtgccaagcg gcggcgtcga gagcgaaccg
      181 tcgtcgaaca cgaagaagac gttcatgccg ccgagttcct cgatgtagcg gcgctcgacc
      241 gcatcgagga aaacgacctg atcgcagcca tgatcgatgg cctcggcctg cgcgcgcagg
      301 ctcgcggcgt aattgccgcc gcatttgacg gcgccggtgc cgccgatcgc ggcgcgcgtg
      361 tagttctccg acacccagat cgacacgggc gcaggtccgc ccttgaaata ggagccgacc
      421 ggcgaggcga tgacggcgaa gatgtattcc gccgatggtt tcacgccaag gaagacctcg
      481 ctcgcgatca tgaagggccg caaatagaga ctgccctcgc cgcctggcat ccaggcgcgg
      541 tcgatgcgca cgagctgctc gatcgcttcg atgaacacag gctcgggcag cgccgccatg
      601 gccatgcgct cagccgaatt ccggaagcgc cgggcattgg cgtcggggcg gaacaggttc
      661 acgccgccgt cgtcgcgctt gtaggccttg aggccttcga aaatctcctg cgcgtagtgc
      721 aggacggctg tggccggatc gagcgggaaa ttcgcgcgcg attcgacgcg cgcgccatgc
      781 cagccgttcg cctgattgta gcggaccaca gccatgtgat cggtgaaaat ccggccgaag
      841 cccgggtcca cgagccttgc cgcgcgctca ttctcggacg tcggattcgt cgcgggctgg
      901 atttcgaatt ccaaactcat tgccttgctt cccgctgtcg gaaccggcgc gattgccgcc
      961 gccggcttgg ctctccgggc ctgctcgaac gaaactgcca tgggttttct cccggattga
     1021 cgctgctcgc gcgcgcccgc gccgtggcgc tgttggttac gcctggcctt caccgccacc
     1081 cgctttgcag ccggtttccg tcccggcatg gtccccagga catgcttttg cggaaggcgg
     1141 aagtccagta tgtttgccga aatgccgctc gacaatcgca cggtagacaa attcgcggcg
     1201 ctggccgcct gctctgcttt tctccggccg cttgaaacat caccgcgggc gaaacgactt
     1261 attgtttcgt cgcggctgat ggttttgtaa cattgaaccc aagatacgtc aatatggctg
     1321 acataaattt ctcacgaccg tcgcccgggc ctgattcgag gccagccgag gggcggtcgc
     1381 acagcccccg ccccggcgat ttgcgctggg atatcatcga gctgttgttc tttgcctacc
     1441 gggactttgt cggcgacgcc gatcacgagc tggaggcctt cgggttcggc cgcgcccatc
     1501 accgggtgct gcatttcgtc catcgctatc ccggcttgaa agtggccgac ctgctggacg
     1561 tgctgcgcat caccaagcag tcgctaggac gggtcctcaa gcaattgctc gatcaaggct
     1621 acatcgtgca gaagaccggc aacaacgacc gcaggcagcg ccttcttttt gccaccgcaa
     1681 agggcgaggc gctggtcgca aagctcgcgg ggctgcagac cgaccgcatc aaccgcgcgc
     1741 tgcgcgacat tggatcatcg ggtgcggata ccgtccgcca gttcctgcgc gcgatgatcg
     1801 atcacgacga tcctgacaag gtgctggaaa cgatcctgag agccggcaac gcaaccgcaa
     1861 aggagtgatc gtgcccttgg ctgcaacaat tgctcgcacc ctgccgcaac cggccgacga
     1921 cgccccgcat atcctgctgg tcgatgacga ccgtcgcatc cgcgatctgt tgtcgcgttt
     1981 tctgtccggc gagggttacc gcgtcaccac tgcgatgagc gccagcgacg cccgcgccaa
     2041 gctgctcggg ctgcatttcg atctcctgat cctcgacgtg atgatgcccg gcgaaaccgg
     2101 gttcgatctg gcccgcttca tccgctcatc atcgtcggtg ccgatcatca tgctgacggc
     2161 gcgccatgag gcggaagccc ggatcgaggg cctgcagatc ggcgccgacg attacgtcgc
     2221 caagccgttc gagccgcgcg agctggtgct ccggatcggc aatattctca agcgcaccgc
     2281 gccgccgccg gtgacggcgc tggagcaggt cgcgttcggt ccttacgttt accaccttga
     2341 ccgcggcgag ttgcgccagg gcgatgaggc cgtgcatctc accgaccgcg agcgcgagat
     2401 gctgcgcatt ctcgccgtca cgccgggcga gaccgtgccg cgcagtgcac tgaccggcga
     2461 gggcaccgtc aacgagcgcg cggtcgacgt gcagatcaac cggctccggc gcaagataga
     2521 acgcgatccc gccaatccgc tgttcctgca ggcggtacgc ggcatcggct atcgcctggt
     2581 cgcatcgccc tgagcagatt agagatgagc acgctcgata ccgggttgac gctgatccgc
     2641 accgccgcgg gccgcgtctc cgcggccaat ggctggatgg gcaatgcctt caagagctgg
     2701 atgccgaccg gactctatgc ccgcgcgctc ttgatcatga tcgtaccgat ggtggtgctg
     2761 cagacggtgg tggcgttcgt gttcatggag cgccactgga acacggtgac gcggcggctt
     2821 tcggccgccg tggtgcagga catcgccagc ctgatcgacg tctacaggat ctacccgcag
     2881 gacaaagatc gcgcgcagtt acgccgcatc gggcagcggc tgcagctggt ggtggatttc
     2941 ctccccgtcg gcgacctgcc gccgcccggc ccaaagccgt tcttctcgct gctcgaccag
     3001 acgctttcgg tgcagcttgg ccggcagatt gcacgaccat tctggatcga taccgtcggc
     3061 cgctccaatc tcgtggaaat ccgcatccag ctcgacgacg cggtgatgcg catcttcgcc
     3121 cagcgcagct cggcctatgc ctcgaattcg gagattttta tcttctggat gctggggacg
     3181 tcgtcgatct tgctgatcgt cgcggtgttg ttcctgcgta accagatccg gccgatcctg
     3241 cgccttgccg acgccgccga aagcttcggc aagggccgcg aggcgccgaa tttccggccc
     3301 cgcggcgccc gcgaagtgcg ccgcgccgcg caggcgttcc tggagatgaa atcgcgcatc
     3361 gagcgctcga tcgagcagcg caccgcgatg ctggccggcg tgtcgcacga tctgcgcacc
     3421 atcctgaccc gcttcaagct cgaactggaa ttgatcggcg atagtcccga ggtcgacggc
     3481 atgcgcaagg atgtcgacga gatgtccggt atgctggagg attatctcgc tttcgcgcgc
     3541 ggcgacactg gcgagcaggc gcaaccgacc gacatggcgc tggcgctcga ggagttgcgc
     3601 agcgacgccg agcgcaacgg ccacgccgcg acggtcgcgt ttcacggact gccggtcgtg
     3661 acggtgaagc cggcatcgtt caagcgctgc ctcgccaacc tggtatccaa cgcggcgcgg
     3721 cacgcgaacg cgatctcgat caccggccac cgcgatcacc gctatctcac cgtcaccgtc
     3781 gacgacgacg gaccgggcat tccgcaggag atgcgcgagg aagtgttcaa gccgttcctg
     3841 cggctggacg acgcccgcaa ccaggatgag ggcggcaccg ggcttgggct tgccatcgcc
     3901 cgcgacatcg cgcgctcgca tggcggcgac atcatgctcg gcgacagccc gatgggcggg
     3961 ctgcgcgcga cggcgcggat accggtgtga cgccgatcgc acggcgcttc agatcggatg
     4021 ctccggtcta gtacaatcct gtttagtaca gggtgcgtag cggcagttcg gtggcgccat
     4081 agggcaccca acggcaggcg aaggaaatgt agccgccgta ttgagggtcg acggagagga
     4141 atttcaccac cttgccgtaa gccgcgcagt gagccaccgc gagctg
//
LOCUS       DATNOI010000703         6655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1357344,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000703 DATNOI010000000
VERSION     DATNOI010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357344"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VN968_28050"
     CDS             <1..180
                     /locus_tag="VN968_28050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33122.1"
                     /translation="AAIVGKARVDDGALADLSWNVLESEIGTAAIAYLETSIRAGAKP
                     IRPARIRTSGTRKAR"
     gene            complement(847..1878)
                     /locus_tag="VN968_28055"
     CDS             complement(847..1878)
                     /locus_tag="VN968_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXL33123.1"
                     /translation="MTYCFGLYGAVLLAAVALSGGAMAQTPAAPSPALQPLGIGLEGI
                     DYPYPVHHLDLTIEGQPLRMAYMDVAPAAAPNDKTVVLLHGKSFSGDYWAHTIATLTG
                     QGYRVVVPDQIGFGKSSKPDIRYHFDLLARNTKMLLDSLGVTRAAIVGHSFGGMLSVY
                     FARDYPETTAVLALENPIGLEDYRSAIPPQPVEALIKTEMAQTPQSYRAFMAAFFVGW
                     PVAAQHYVDIFSRVLQSPDYPRWARASALTYDMIFNEPIRHEYHLLKMPVLLVIGQSD
                     RSVFFRRYASPDAIKPLGNWPVLGREALKDLPDGKLVEIAESGHVSHIEKPDEFDAAL
                     TAFLTTRFR"
     gene            complement(2045..2650)
                     /locus_tag="VN968_28060"
     CDS             complement(2045..2650)
                     /locus_tag="VN968_28060"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXL33124.1"
                     /translation="MKILRVQTSPRGENSESRKLGDHLIAKAKRIDPTSTTILRDVST
                     GLALPDLTWIGASFTDADKRDANQVRALAGSEQLIAELEATDLLVIEAPLWNFSIPAS
                     LKAWFDQVVRARRTFRMSETGSEGLLKDRKTIVIVTTGGVPLGSGYDFATPYIRHILG
                     FIGITGVTFIDAGRLMFENDKLDKARVEINTTLAGPLARAA"
     gene            complement(2709..3707)
                     /locus_tag="VN968_28065"
     CDS             complement(2709..3707)
                     /locus_tag="VN968_28065"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXL33125.1"
                     /translation="MGLLVEGRWQDVWYPTESTGGRFVRKDAAFRNWITVDGSAGPTG
                     IGGFKSEAGRYHLYVSLACPWAHRTLIMRMLKGLEAKISVSVAHWLMLDHGWTFADGP
                     GVVPDTVNHAKFLHEIYTAADPHYTGRATVPVLWDKHRRTIVNNESSEIIRMLNSEFD
                     EVGARPGDYYPQALRADIDAVNARIYDTLNNGVYKAGFATTQAAYEKAVLPLFETLDW
                     LEQRLADRRFLFGNNLTEADIRLFTTLIRFDAVYVGHFKCNIRRLVDYRNLSAYARDI
                     YQSPGVAATVNFEHIRRHYYESHRSINPSGIVPAGPVLDFTSPHGRERLAVAPLAQ"
     gene            complement(3744..4526)
                     /locus_tag="VN968_28070"
     CDS             complement(3744..4526)
                     /locus_tag="VN968_28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL33126.1"
                     /translation="MTQETAQGRLQDKVAIVTGASSGIGRATAKLFAAEGAKVVVGAR
                     REAELAGLVAEIKGSGGSAVSLAGDVRSEDYAKALVALAVKTYGRLDVAFNNAGTLGE
                     AGPSTGVSEAGWSDAIAINLTGSFLGAKHQIAEMVKHGGGSVIFTSTFVGHTFAFPGV
                     AAYAASKSGLIGLTQALAAEFGPQNVRVNAVLPGAVDTDMYREMNDTPDKTSFITNLH
                     ALKRVAKPEELARSVLYLASDDAAFVTGTASLVDGGASITRT"
     gene            4642..5559
                     /locus_tag="VN968_28075"
     CDS             4642..5559
                     /locus_tag="VN968_28075"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXL33127.1"
                     /translation="MIGWGMDRLRAFEVFATVVGQGSFTRAADKLETSPANVTRYVNE
                     LEESLGARLLNRTSRRLSLTETGKTLYDKALSILEDVAEAEAIASSAALQPRGRLRVN
                     APLSFGILHLAPLWPRFMTRYPDIELDISLVDRVVDLVEEGYDLAVRISRGGSPALVS
                     RKLATARHLICASPDYVARHGAPESPEDLNRHACIAYTYSDSADEWRLLDDAGKVHSV
                     AVPNIMQTNNGDTARAAALAGLGIIRQPTFLIGEDLRQGRLVPLLTGYRMSEIDVLAV
                     YPSRRHLSAKVRVMVDFLVEAFRGTPLWD"
     gene            complement(5776..>6655)
                     /locus_tag="VN968_28080"
     CDS             complement(5776..>6655)
                     /locus_tag="VN968_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXL33128.1"
                     /translation="IQWLLKNHRDLIDAEFALNEGGGVGLKDGKPIRNSVQTSEKVSL
                     SYQLDVKNKGGHSSVPSRDNAIYRLAEGLVRLSKFSFPLKLNETTRAYFERTAQFEGE
                     QTAADMRAVLSDRPDPAAMSFVRLAANPVYNAQLRTTCVATMLQGGHAVNALPQLASA
                     KVNCRIMPGEPVDEVKATLERVLADDQIVVTQLDQPVLSAPSALHEEIMGSIEKLSQE
                     FWPGAVVLPTMSAGATDGSYLRNAGIPTYGHSGLAGDINDIRAHGKDERILVKSFHEG
                     EDYLYRLVKMLSGGGS"
BASE COUNT         1216 a         1994 c         2138 g         1307 t
ORIGIN      
        1 gccgcgatcg tgggcaaggc ccgcgttgac gacggagcgc tcgccgatct gtcatggaat
       61 gtattggaat ctgagatcgg aacggctgct attgcatatc tcgaaacgag cattcgtgct
      121 ggtgccaagc caatcaggcc agcaagaata aggacatcgg ggacgaggaa ggccagatga
      181 tgcgcgatgg tcgccaacta tccagatcga tcatctgacg ccgaaaaaac tttgcgatcc
      241 tctcaatcaa gagacgcaca cgcttcggct gacctcccac tcacgaatga ccgctttgcg
      301 ccagaagcgg ccgctccata accgctcatt tcgcgaacaa gcagtagcac tccgtaccgc
      361 gccgatcata gaatttacga ggtccgcttt tgtgccgaaa gcgcaagttc accgcgatag
      421 gcggcacttc cgaaaaatgc cagaaaggag actacaaggt gttgaacgaa tttctgctga
      481 ctgagccaat gcggctaatg gaaaagcgct tccacgggag cacgcatttt gagtcaggcc
      541 gtgaagcagt tgtcgaccat gccttccggc cagcgcgcca tcgtcagcat ttgcgccgac
      601 ggtgggcaag gtacggtcgc gctgctgcag gcggcctgat cgatacccat tacgaaccag
      661 cagcggtcct tcaagcgctc cgtccttcag caggccctgc atcaacacga tatcgccgga
      721 atcgagaagt tcgatttcgg ggcgaaagcc agctttccgg gcacggggtt ttgggccaag
      781 acggcttgac ttggtttgca aaacttgacg acaaggcttg gcgagcaagg tctgggcacg
      841 aaggggtcag cgaaaccggg tcgtcaggaa cgccgtgagg gcagcatcga attcgtccgg
      901 cttttcgatg tgcgagacgt gaccggattc ggcgatttcg accagcttgc cgtcgggaag
      961 atccttgagc gcttcgcggc cgagcaccgg ccagttgccg agcggcttga tcgcgtcggg
     1021 cgaggcgtag cggcggaaga agacgctgcg gtcgctctgc ccgatgacga gcaagaccgg
     1081 catcttcaac agatggtatt cgtggcggat cggctcgttg aagatcatgt cgtaagtgag
     1141 cgccgaggcc cgcgcccagc gcggataatc tggactttgc aatacccgcg aaaagatatc
     1201 gacatagtgc tgcgcggcca ccggccagcc gacgaagaag gccgccatga aggcgcgata
     1261 gctctgcggc gtctgcgcca tctcggtctt gatcagcgcc tcgaccggct gcggcgggat
     1321 ggcgctgcga tagtcctcaa ggccgatcgg gttctccagc gccagcactg ccgtcgtctc
     1381 cggatagtcg cgcgcgaaat agactgagag catgccgccg aaggaatggc cgacgatcgc
     1441 cgcgcgggtc actccgaggc tgtcgagcag catcttggtg ttgcgggcga ggagatcgaa
     1501 atgatagcgg atgtcgggct tcgacgattt gccgaagccg atctggtccg gcacgaccac
     1561 gcgatagccc tggccggtca gcgtcgcgat cgtgtgcgcc cagtaatcgc cggaaaagct
     1621 tttgccgtgc agcagcacga cggttttgtc gttcggcgcc gcggccggcg ccacatccat
     1681 gtaggccatg cgcagcggct ggccctcgat ggtgagatcc agatggtgca ctgggtaggg
     1741 ataatcgatg ccctcgaggc cgatgccgag cggttgcaag gcgggcgatg gagcggccgg
     1801 tgtctgcgcc atggcgccgc cgctcagcgc gacggccgca aggaggacag cgccatacag
     1861 tccgaaacaa taagtcatgg tggattacct cattcaaaag tcattttgcc gacggacgtg
     1921 tttgcgagag gcccgggcca ttgcggagcg gacgcgggcg tcccggcgag accggtcttc
     1981 ctgcgcgttc cgtgcgcgca ccgcgccccg gacagcgatc cgcgggcgcg gcacgattgc
     2041 cgtgttacgc ggccctcgcc agcggcccgg cgagggtcgt gttgatttcg acgcgggcct
     2101 tgtcgagctt gtcgttttcg aacatcagcc gcccggcatc gatgaaggtg acgccggtga
     2161 tgccgatgaa gccgaggatg tgccggatat agggggtagc gaaatcataa ccgctgccga
     2221 gcggaacgcc gccggtcgta acgatgacga tggtcttgcg atccttcagc aggccctcgg
     2281 agccggtctc cgacatccgg aaggtgcggc gcgcccgcac cacttggtcg aaccatgcct
     2341 tcaggctcgc agggatcgag aagttccaca gcggggcctc gatgacgagt agatcggtcg
     2401 cctcgagttc ggctatcagt tgttcggagc cggccagggc gcggacttgg ttcgcgtcgc
     2461 gcttgtcggc gtcggtgaag ctggcgccga tccaagtgag atcgggcagc gcgaggccgg
     2521 tcgatacgtc acgaaggatc gtggtggaag tgggatcgat ccgcttggcc ttcgcgatga
     2581 gatggtcgcc gagtttgcga ctttccgagt tttctccgcg aggactggtc tgtactctca
     2641 ggatcttcat gacactctcc atatgcgtgt ggcaggttga tgattttgtt caggcgactg
     2701 cagttgtttc attgtgcaag cggcgcgacg gcgagccgtt cgcgtccgtg gggtgatgtg
     2761 aaatcgagca cggggccggc gggaacgatc ccgctcggat tgatcgagcg gtgactctca
     2821 tagtagtgcc gccggatatg ctcgaaattc accgtcgcgg cgacgccggg cgattgatag
     2881 atgtcgcgcg cataggccga cagattgcga taatcgacca gccggcggat gttgcacttg
     2941 aagtgtccga catagaccgc gtcgaagcgg atcagcgtcg tgaacagccg gatgtcggcc
     3001 tcggtcaagt tgttaccgaa cagaaagcgc cggtcggcga gccgttgctc aagccaatcg
     3061 agcgtttcga acagcgggag caccgctttt tcataggcgg cttgcgtggt cgcgaagccc
     3121 gccttataga cgccgttgtt gagcgtgtcg tagatccgag cattgaccgc gtcgatgtcg
     3181 gcgcgcagcg cttgcgggta gtagtcgccg ggtcttgctc cgacctcatc gaactccgaa
     3241 ttcagcatcc ggatgatctc ggaggattcg ttattgacga tggtgcgcct gtgcttgtcc
     3301 cacagcaccg gaactgtggc gcggccggtg tagtgcggat ccgcagcggt gtagatctca
     3361 tgcaggaatt tggcatggtt caccgtatcc gggacgacgc cggggccatc ggcgaaggtc
     3421 cagccgtgat ccagcatcag ccagtgcgcc accgacaccg agatcttggc ttcgagccct
     3481 ttcaacatcc gcatgatcag cgtgcggtgc gcccaggggc aggcgaggct gacatagagg
     3541 tggtagcggc cggcttcgga cttgaagccg ccgatgccgg tcggaccggc gctgccgtcg
     3601 acggtgatcc agttgcggaa cgccgcgtcc ttgcgcacga agcggccgcc ggtcgactcg
     3661 gtcggatacc agacgtcctg ccatcgacct tcaacgagca atcccatgtc agcctcctca
     3721 gtgtttccct ttgatgcgcc ggttcaggtg cgggtgatcg aggcgccgcc atcgaccagc
     3781 gaggcggtgc cggtcacgaa agccgcatcg tcggaggcga ggtacagcac cgaccgcgcc
     3841 aactcttccg gcttggccac ccgcttcagt gcgtgcaggt tggtgatgaa actggtcttg
     3901 tccggcgtgt cgttcatctc ccggtacatg tcggtatcga ccgcgcccgg cagcaccgca
     3961 ttgacgcgta cgttctgcgg gccgaactcg gcggcgagcg cttgcgtcag tccgatcagg
     4021 ccggacttac tcgcggcgta ggcagcaaca ccggggaaag cgaaggtgtg tccgacgaat
     4081 gtcgaggtga agatcaccga gccgccgcca tgcttgacca tctcggcgat ctgatgcttg
     4141 gcgccaagga acgagccggt gaggttgatc gcgatggcat cgctccagcc ggcctcggat
     4201 acgccggtcg aaggaccggc ctcgccgaga gtgcccgcat tgttgaaggc gacgtcgagc
     4261 cggccatagg tctttaccgc cagcgccacc agcgccttgg cataatcctc cgagcggacg
     4321 tcgccggcga gcgacacggc gctgccaccg ctgcccttga tctcagccac caagcccgcc
     4381 agttcggcct cgcggcgggc gccgacgacg accttggcgc cttcggccgc gaacagcttg
     4441 gcggtggctc ggccgatgcc cgagcttgcg ccggtgacga tggctacctt gtcctgaaga
     4501 cgtccctgtg cggtttcctg tgtcatggtc gttgtccttg ttcctcggcg gcgtcgatcg
     4561 ccgcgtgatg ggatgtgagg tagacccgtc ccggacttgg aagaaggcac ggaaactggt
     4621 tacatcattc atttttcatg aatgatcggg tggggcatgg atcgtctgcg ggcttttgag
     4681 gtattcgcga ccgtggtcgg ccagggcagc ttcacccgcg ccgcggacaa gctggagacc
     4741 tcgccggcca atgtcacccg ttatgtcaac gagttggagg agtcccttgg cgcgcggctg
     4801 ctcaaccgca cctcccggcg cctgtcgctg accgagaccg gcaagacgct ctacgataag
     4861 gcgctctcca ttctcgagga tgtggcggag gccgaggcga tcgcgtcgtc cgcggccctg
     4921 cagccgcgcg gacggttgcg ggtgaacgcg ccgctctcgt tcggcattct gcatctggcg
     4981 ccgctttggc cgcgcttcat gacgcgctat ccggacatcg aactggacat cagcctggtc
     5041 gatcgcgtcg tcgatcttgt cgaggaaggt tatgatctgg ccgtgcgtat ctcgcgcggc
     5101 ggatcgccag cccttgtcag ccgcaagctc gcgaccgcgc gtcatctgat ctgtgcatcg
     5161 ccggactatg tcgcgcgcca cggcgcgccc gaaagtcccg aggatctcaa ccgccacgcc
     5221 tgcatcgcct acacctattc ggattcagcc gatgagtggc ggctattgga tgatgcaggc
     5281 aaggtgcatt ccgtcgcggt gcccaacatc atgcagacca acaatggcga taccgctcgc
     5341 gcggcggcgc tggccggcct cggcatcatc aggcagccga ccttcctgat cggcgaggat
     5401 ttgcggcagg gccggctggt gcccttgctc accggctacc gcatgtccga gatcgacgtg
     5461 ctggcagtct atccgagccg ccgccacctc agcgcgaagg tgcgcgtcat ggtggacttt
     5521 ttggtcgagg cattccgggg cacgccgcta tgggactaaa ggggcgggat tgacaggcgc
     5581 atcgtgatgc cgcgaaggcc gtctgctcgc agcgtttggg gccccactga cgacggtcga
     5641 gtccgttgta ggtccctcag aagcagacgt tccatagcca acattatgcg aatccgacat
     5701 gccgccgccg caatcgggca ctacacgctt atttaaatgt tccggctttt tcgagattgc
     5761 gaactcagcc cttatctacg agccgccgcc ggacaacatt ttaaccaatc gatagagata
     5821 atcctcacct tcgtggaacg atttcacgag aatccgttcg tcctttccgt gtgcccgaat
     5881 gtcgttgata tcacccgcga gcccggagtg gccatacgtc ggaatgccgg cgtttcgtag
     5941 gtagcttcca tcggtggcac cggcgctcat cgtcggaagc acgacagcgc cgggccaaaa
     6001 ctcctgcgat aatttctcaa tcgatcccat gatctcctcg tgcagtgctg acggcgcact
     6061 caaaaccggt tggtcgagtt gcgtgacaac gatctgatcg tccgcgagca cgcgctccag
     6121 ggtcgctttg acctcatcga cgggttcgcc gggcatgatc cggcaattga ctttggcgct
     6181 cgccagttgc ggcaacgcgt tgaccgcgtg tccgccttgc agcattgtcg cgacgcaggt
     6241 ggtgcgcaat tgggcgttat agaccggatt ggctgcgagc cggacgaatg acatggcggc
     6301 cggatccggc cgatcggaca gcaccgcgcg catatcggcc gcggtctgct cgccctcgaa
     6361 ttgcgcggtg cgctcaaagt aagcgcgcgt ggtctcgttg agcttcagcg ggaagctgaa
     6421 tttggagaga cgtaccagtc cctccgccag acgatagatc gcattgtccc tgctcgggac
     6481 cgagctgtgg ccgcccttat ttttcacgtc gagctgataa ctcagcgaga ctttctcgct
     6541 ggtctggacg ctgttcctga tcggcttgcc gtccttcagt ccgacgccgc cgccctcgtt
     6601 caatgcgaat tcggcatcga tcaggtcgcg atggttcttg agcagccatt ggatt
//
LOCUS       DATNOI010000704         2154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_128411,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000704 DATNOI010000000
VERSION     DATNOI010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128411"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VN968_28085"
     CDS             <1..940
                     /locus_tag="VN968_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL33129.1"
                     /translation="ASWLAGGKQILIDRVEWIVIPDPATAAAALQNGEVDWWENPITD
                     LVPVLKKNRNISVDIADPLGNIGVFRMNHLHPPFNDVKARRAILTALSQEDYMRALVG
                     DDTSLWKPLPGFFTPDTPLYTEEGGEILKGKRDIAAAKKLLAESGYSGQPVSCIVAQD
                     QPITKAQGDVTADLLKHLDMNVDFVATDWGTVGSRRAQKTPPGQGGWQMFHTWHAGAD
                     CVNPAAYAGIRGNGDKAWWGWPTSPTVEKEVTAWFDAKNLDEEKAAVGRLNKAALEDV
                     IYAPTGFFLSYTAWRKNISGIVKGPLPFFWDVSKSA"
     gene            958..1899
                     /locus_tag="VN968_28090"
     CDS             958..1899
                     /locus_tag="VN968_28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL33130.1"
                     /translation="MVSYILRRILSTLPVMAIVALFVFSLLYIAPGDPAVVIAGDQAS
                     PADVERIRQSLGLDRPFLVQFGTWVWHILHGDLGTSIFTSLPVSALIAQRIEPTLSLM
                     VITLILTILIAVPLGVVAAWKAGSWVDRTIMGFAVFGFSVPVFVVGYVLAYVFALEFE
                     WLPVQGYTPLAQGLWPWLQNLILPAVALGCVYIALIARVTRASMLEVLQQDYIRTARA
                     KGLGQRSILFIHALKNAAVPVVTVIGIGVALLIGGAVVTESVFAIPGLGRLTIDAILR
                     RDYPVIQGIVLLFSFTYVLVNLMVDVTYTLVDPRIRY"
     gene            1920..>2154
                     /locus_tag="VN968_28095"
     CDS             1920..>2154
                     /locus_tag="VN968_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXL33131.1"
                     /translation="MPVPQGLVIAPQLPDLLLPVKVRRGILGFLRNNPTVAIGGALLL
                     CLVLVGIFAPLLGTVDPTALAPAKRTRAPSADFW"
BASE COUNT          355 a          685 c          679 g          435 t
ORIGIN      
        1 ggcgtcctgg ctcgccggcg gcaagcagat tttgatcgac cgcgtcgaat ggatcgtgat
       61 cccggatcct gccacggcgg cggccgcgct gcagaacggg gaggtcgatt ggtgggagaa
      121 tccgatcaca gatctcgtgc ccgtgctgaa gaagaaccgc aatatcagcg tcgatatcgc
      181 cgacccgctc ggcaacatcg gtgtcttccg gatgaaccac ctgcacccgc ccttcaacga
      241 tgtgaaagcg cgacgtgcca tactcacggc cctcagccag gaagactaca tgcgtgcgct
      301 ggtcggggat gatacctcgc tctggaaacc gctgccgggt ttcttcaccc cggatacgcc
      361 gctctacacg gaggagggcg gcgagatcct gaagggcaag cgcgatatcg cagcggccaa
      421 gaagctcttg gccgagagcg gctattcggg ccagccggtc tcgtgcatcg tggcgcagga
      481 tcaaccgatc accaaagcgc agggcgatgt caccgcggat ctgttgaaac atctggacat
      541 gaatgtcgac ttcgtcgcga ccgattgggg cactgtcggt tctcgccgcg cccagaagac
      601 gcctccgggc caggggggct ggcagatgtt tcacacctgg cacgccggcg cagactgcgt
      661 caatccggcc gcctacgctg gcattcgcgg caatggcgac aaggcctggt gggggtggcc
      721 cacgagtcca accgtggaaa aggaagtcac ggcatggttc gacgccaaga atctcgatga
      781 agagaaggcc gcggtcggtc gcctcaacaa ggcggcgctc gaggacgtca tttatgcacc
      841 gaccggcttc ttcctgagct acaccgcgtg gcgcaagaac atctccggca tcgtgaaggg
      901 gccgctgccg ttcttctggg acgtgtcgaa gagtgcatga cgatgcggtg aaccgacatg
      961 gtctcctata ttctccggcg tattctctcg accttgcccg tgatggcgat cgtcgcgctg
     1021 ttcgtcttca gcctgctcta catcgcgccg ggcgatccgg cggtggtgat cgcgggcgac
     1081 caggcgagtc ccgccgacgt tgagcgcata cgccagagcc tcggcctcga ccggccgttt
     1141 ctggtccagt tcgggacctg ggtctggcat atcctgcacg gcgatctcgg cacctcgatc
     1201 ttcacgagcc tgccggtttc cgcgctgatc gcgcagcgta tcgagccgac actgtcgctg
     1261 atggtgatca ccctcatcct gaccatcctc atcgccgtgc cgctcggtgt agtggcggcg
     1321 tggaaggcag gaagctgggt cgaccgcacc atcatgggat ttgccgtgtt cggcttctcg
     1381 gttcccgtct tcgtggtcgg ctacgtcctt gcttatgtgt tcgcgctcga gttcgaatgg
     1441 cttcccgtgc agggctacac gccgctcgcc caaggtcttt ggccgtggct gcaaaacctg
     1501 atcctgccgg cggtcgcgct cggctgcgtt tacatcgcgc tgatcgcgcg agttacccgc
     1561 gcttcgatgc tcgaggtatt gcagcaggat tatatccgaa ccgcgcgggc caagggtctg
     1621 gggcagcgca gcatcctgtt catccacgcg ctgaaaaatg cggccgttcc ggtcgtgacg
     1681 gtgatcggta tcggcgttgc gctgctgatc ggcggcgcgg tcgtgaccga aagcgtgttc
     1741 gcgatcccgg gcctcggccg attgacgatc gacgccatcc tgcgccgcga ctatccggtg
     1801 atccagggca tcgtgctgct gttcagcttt acctatgtgt tggtcaatct gatggtcgac
     1861 gtgacctaca ccctggtcga tccgaggatc cgctattgac cgtctcagcc acctctacga
     1921 tgccggtgcc gcagggtctt gtcatcgcgc cgcaactgcc ggacctgctg ctgccggtga
     1981 aagtccggcg cggtatcctc ggcttcctgc gcaacaatcc gaccgtcgcg atcggcggcg
     2041 cattgctgct ctgcctggta ttggtcggga tcttcgcgcc cttgctcggg accgtcgacc
     2101 cgaccgcact ggcgcccgcc aagcgcacgc gcgcgccctc cgctgacttt tggt
//
LOCUS       DATNOI010000705         4151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1836523,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000705 DATNOI010000000
VERSION     DATNOI010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1836523"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1479)
                     /locus_tag="VN968_28100"
     CDS             complement(<1..1479)
                     /locus_tag="VN968_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXL33132.1"
                     /translation="MSGAAPSQSGGHRIIIPLLVISDATAAILLAADLLVVCGSVLLR
                     FLFNAPVEWADDVARGLMVGSSFFGAASALARAENLGVAFFIDMLPAGIRRVVDAVGA
                     LLVTVISGYVAFNAIKLGWLTTGQTSGSGLPLELTFYPMGAGALFMTVFAANLFCARP
                     VRDIIGGTVATGLIVGLYLAWDFLGPASVPSSGTLMLVGFFITLFGGLPIGFALALAA
                     LTFIWVEGTLPGVIFAQQMARGIDNFVLLAIPFFILVGYLMEANGMSVRLIELLQRAV
                     GRMRGGLNVVMVTSMVLFSGISGSKMADVAAVGSVLIPAARRSRQNPGSAVALLAASA
                     VMAETIPPCINLIILGFVANLSIGGLFVAGLLPASLMALALIAVSIIFGKRAASVKEI
                     EPQIPMSGLWSGAIASFGLIFMIFAGFKSGFATATEISAFAVAYAIVIGSLVFRELSF
                     KTAASSFVQAATRSGLVLFIVAAAQSLAFVLTLQQVPHAVGDL"
     gene            complement(1491..2522)
                     /locus_tag="VN968_28105"
     CDS             complement(1491..2522)
                     /locus_tag="VN968_28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXL33133.1"
                     /translation="MKPVSVGRRLFLQSSTFAVTWLAASPAIIGRAQAATMKMKCSSS
                     LPNDPKYANGRVYYDHLVKNLKANGLGEQIEVAFFPDNQLGQEIDIINSVKLGVIDLM
                     VSGSSISANLVPLVGTFDLGYLFSSFPQQTKAFDAGASKPIEDALLKGASIRIISWAY
                     NFGSRSVLAKKPVKGPEDLAGLKIRTLPNPVITECLRLMGAAATPLAFGEIYTALQAG
                     VLDGLEHDPPTILASKFYETSKHYALTQHNFSPLATYFSEATFNRMDPKLREGFLDAA
                     KKAAADTRTHGLEMEKEALKNLVDKGVTIVECDKEAFRKRVLPQTDNFVKARPEAKPV
                     VDMIRATQA"
     gene            complement(2682..3431)
                     /locus_tag="VN968_28110"
     CDS             complement(2682..3431)
                     /locus_tag="VN968_28110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL33134.1"
                     /translation="MLTSAPEPLLAVGRLESGYGKIRVLHGIDLTVSPGEVVALLGPN
                     GAGKTTMLRALSGLLPVNAGHVRFGGRDMTNATPREAARAGLVHVIEGHRVFTQISVT
                     DNLLLAGYDLPRGERAARVEEALSFFPEIAEKRHERGGALSGGQQQMLTVAQGLVRRP
                     RLLMLDEPSAGLSPVLVNRVLNVVGQLRTQGTAVLLVEQLLEKALAAADRVYALVQGH
                     IVLEAPTGEANLPQRIERAYFGHEAHRVDVE"
     gene            complement(3412..>4151)
                     /locus_tag="VN968_28115"
     CDS             complement(3412..>4151)
                     /locus_tag="VN968_28115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXL33135.1"
                     /translation="RGIALSFGNVRAIDGLDLDVKPGQIHGLIGPNGSGKTTTLNVIS
                     GYYAAKAGTMTLGEDMLPPGMPALRASRGIARTFQTPRVIGEASVLQNVMIGGTIEGR
                     ATFVEALLALPRNQRDERMLGAKARALLGVVGLETLADVRADRLQHSELRFIEIARAL
                     MLEPDFLLLDEPAAGLSSDEIERLAALIKAISRRGAGVLLVEHHADLIFDICDQVTVL
                     NLGRTLAAGTPAEIRVHKEVVSAYLGA"
BASE COUNT          834 a         1322 c         1303 g          692 t
ORIGIN      
        1 caagtcgcct accgcatgcg gcacttgctg cagcgtaagg acgaaagcga gcgattgcgc
       61 ggcggcgacg ataaacaaca ccaatccgga gcgtgtggcg gcctgcacga agctggacgc
      121 cgcggtcttg aagctgagtt cacgaaacac cagactgccg atgacgatgg cataggccac
      181 ggcgaacgcc gaaatctccg tcgcggtggc aaagccgctc ttgaagccgg cgaagatcat
      241 gaagatcaag ccgaacgatg cgatcgcgcc gctccaaagg cccgacatcg gaatctgcgg
      301 ttcgatctcc ttgaccgatg cagcacgttt tccgaagatg atcgacaccg cgatcagcgc
      361 cagcgccatc aggctggccg gcaacaagcc ggcgacgaac aggccgccga tcgacaggtt
      421 ggcgacgaat cccaggatga tcaggttgat gcagggtgga atggtttccg ccatcacggc
      481 agacgccgcc agcaatgcca ccgcgctgcc cggattctgc cgggaacggc gggccgcggg
      541 gatcaggaca gagcccaccg ccgcgacgtc agccatcttg gagccggaga tgcccgaaaa
      601 cagcaccatc gatgtcacca tcaccacgtt caggccgccg cgcatgcgcc ccacggcgcg
      661 ctgcaacagt tcaatcaggc gaaccgacat gccgttcgcc tccatcagat agccgacaag
      721 gatgaagaag ggaatcgcga gtaacacgaa attgtcgatg ccgcgcgcca tctgctgcgc
      781 gaagatgacg ccgggcagcg tgccttccac ccaaatgaaa gtcagggccg ccagcgccag
      841 cgcaaagccg atcggcaatc cgccgaacag cgtgatgaaa aagcccacca gcatcagcgt
      901 tcccgacgac ggcaccgagg ccgggcccag gaaatcccac gccagataga gaccaacgat
      961 caggcccgtc gcaaccgttc cgccgatgat gtcgcggacg gggcgcgcgc aaaaaagatt
     1021 ggccgcaaaa accgtcatga acagcgcgcc ggcacccatc gggtagaaag tcagttccag
     1081 cggcaggccg gagccggagg tctgcccggt ggtcagccag ccgagcttga tggcgttgaa
     1141 cgccacgtag cccgaaatca cggtcacgag cagcgcgccc acggcatcca ccacgcgcct
     1201 gataccggcc ggcagcatgt cgatgaaaaa cgccacgccg agattctcag ctcgggccag
     1261 cgcgctggcg gcgccaaaga agctcgaccc aaccatgagg ccgcgcgcca catcatcggc
     1321 ccattcgacc ggcgcattga acaggaagcg cagcaacacc gaaccgcaga cgaccagaag
     1381 gtcggctgcg aggaggatcg ccgccgttgc gtcgcttatg acaagcagcg ggatgatgat
     1441 ccggtggccg ccgctttgcg aaggcgcggc gcctgacatg gtctgtaccc tcaagcctgg
     1501 gtcgcgcgga tcatgtccac tacgggtttg gcttcggggc gggccttcac gaaattgtcg
     1561 gtctgcggaa gcacacgctt gcggaacgct tccttgtcgc actcgacaat cgtgacgcct
     1621 ttgtccacga gattcttgag cgcttccttc tccatttcaa gaccgtgcgt acgggtatcg
     1681 gccgccgcct ttttggccgc gtcgagaaat ccttcacgca gtttcggatc catccgattg
     1741 aatgtcgctt cgctgaaata cgtcgcgagc ggcgagaaat tatgctgcgt cagcgcgtag
     1801 tgcttggagg tctcgtagaa cttgctggcg aggatggttg gcgggtcatg ctcaagcccg
     1861 tcgagcacgc cggcctgcaa ggccgtgtag atttcaccga acgccagcgg cgtcgcggca
     1921 gcgcccatca ggcgaagaca ctcggtaatg acgggattcg gcagggttct gatcttgagg
     1981 ccggccagat cttcgggacc ctttacaggc ttcttggcaa gcacgctgcg cgagccgaaa
     2041 ttataggccc aggagatgat gcggatactg gcgcccttga gcagagcatc ctcgatcggc
     2101 ttggaagcgc cggcgtcgaa ggctttggtc tgttgtggaa agctcgagaa caggtagccg
     2161 aggtcaaagg tgccgaccag aggcacaaga ttggccgaga tcgatgagcc cgacaccatc
     2221 aggtcgatca caccgagctt gacggaattg atgatatcga tttcctgacc gagctggttg
     2281 tcgggaaaga aagcgacttc gatctgctcg ccaagcccat tggctttcag atttttgacc
     2341 agatggtcgt aatagacccg gccgttggca tatttgggat cgttgggaag cgacgatgaa
     2401 catttcattt tcatcgtcgc agcctgcgca cgcccgatga tggcgggcga ggctgcgagc
     2461 catgtgaccg caaacgtcga cgattgcagg aacagccggc ggccgacgga cacgggtttc
     2521 atggtgtttc ctccagagcg cgttttcttg cgccgctatt ggtttgcggc cgttcgctta
     2581 tcattggtcg caaatgtatg accaaacgga agcgcgttgc aaggcgggaa tattgtcgag
     2641 ggctcaaacg tgccaagcga gacgtcgggc aattcgagcg atcactcaac atccaccctg
     2701 tgcgcttcat gtccgaaata agcgcgctcg atccgctgcg gcaggttcgc ttcgccggtc
     2761 ggcgcttcca gcacgatgtg cccctgcacc agcgcgtaga cccggtcggc ggcggcgagc
     2821 gccttttcca gcaattgctc gaccagcaac accgcggtgc cttgcgtgcg caactggccg
     2881 acgacgttga gcacgcgatt gaccagtacc ggcgacaggc cggccgacgg ttcgtccagc
     2941 atcaacaggc gcgggcggcg taccagtccc tgcgccaccg tcaacatttg ctgttgcccg
     3001 ccgctcagcg cgccgccgcg ctcgtggcgc ttctcggcga tctctggaaa gaacgacagc
     3061 gcttcctcga cccgcgccgc gcgttcgccg cgcggcaggt cgtagcccgc cagcaacaga
     3121 ttgtcggtga cggaaatctg cgtgaacacg cgatgtcctt cgataacgtg cacgagcccg
     3181 gcgcgcgccg cctcgcgcgg cgtggcgttg gtcatgtcgc ggccaccgaa gcgcacatgt
     3241 cccgcgttga ccggcagcaa tcccgacagc gcgcgcagca tggtggtctt gccggcgcca
     3301 ttggggccga gcagcgccac cacctcgccc ggcgacaccg tcaaatcgat accgtgcagc
     3361 acgcggatct tgccgtatcc ggactcaagc cggccgacgg cgagcaatgg ttcaggcgcc
     3421 gaggtaagca ctgaccacct ccttgtggac gcgaatctcg gcaggcgttc cggcggccag
     3481 cgtgcggccg agattgagca cggtgacctg atcgcagatg tcgaagatca ggtcggcatg
     3541 atgctccacc agcagcacac cggccccgcg ccggctgatc gccttgatca gcgccgccag
     3601 acgctcgatc tcgtcgctgg agaggccggc ggcgggttcg tcgagcagca ggaaatccgg
     3661 ctccagcatc agcgcccgcg cgatctcgat gaagcgcagt tcgctgtgct gcagtcggtc
     3721 ggcacggacg tcggccagcg tttcaagtcc gaccacgccg agcagcgccc gggccttggc
     3781 gccaagcatg cgctcgtcgc gctggtttcg tggcagcgcc agcagcgctt cgacgaacgt
     3841 cgcccggccc tcgatggtgc cgccgatcat gacgttctgc agcaccgagg cctcgccgat
     3901 cacgcgcggc gtctggaagg tgcgggcgat gccgcgggag gcacgcaacg caggcatgcc
     3961 cggcggcaac atgtcctctc ccaacgtcat cgtaccggcc ttggcggcgt agtagcccga
     4021 gatcacgttc agggtcgtgg tcttgccgct gccattcggg ccgatcaggc cgtggatctg
     4081 cccgggcttg acatcgagat caaggccgtc gatggcgcgc acattgccga aactcagcgc
     4141 gatgccgcgc a
//
LOCUS       DATNOI010000706        13663 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2595733,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000706 DATNOI010000000
VERSION     DATNOI010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 13663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13663
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2595733"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VN968_28120"
     CDS             <1..357
                     /locus_tag="VN968_28120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXL33136.1"
                     /translation="DSILVPLQCEFFALEGLSQLLQTVEQVRSTLNPSLTIHGIVLTM
                     FDSRNNLSNQVVADVRQFMGNKVYNTMIPRNVRISEAPSYGKPVLVYDLKCVGSEAYL
                     KLATEVIQRERDLRPH"
     gene            535..1413
                     /locus_tag="VN968_28125"
     CDS             535..1413
                     /locus_tag="VN968_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXL33137.1"
                     /translation="MVDDARSRLGRGLASLIGDVGGEAKHQDRPRTQRKVPIEFLKPN
                     PRNPRRDFSDAELGELADSIRQHGVIQPIVVRPVKGSQDRFEIIAGERRWRASQIVGL
                     HEVPIVPVDVSDSDALEIAIIENVQREDLNGMEEAQGYHALANEFKRSQDEIAKIVGK
                     SRSHVANMMRLTKLPAEVQAYIALGQLSAGHARALIGVPDPAAAAKRIVEEGLNVRQA
                     EALAHVEGVPERKPQKARGGKPKDPNTVALEKRVSDALGLAVNVDHRGTGGTVHVRYG
                     DLDQLDEIVRRLEQGS"
     gene            complement(1591..1866)
                     /locus_tag="VN968_28130"
     CDS             complement(1591..1866)
                     /locus_tag="VN968_28130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018068758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33138.1"
                     /translation="MQAFPPRLPDQPIFYPVLSEDYAAKIARDWNVPASGSGFVTRFE
                     VQRTFLDKYKIQKAGGSAHLEYWIPAEDLVAFNEAIVGEIKVVAEFR"
     gene            complement(1977..3005)
                     /gene="holA"
                     /locus_tag="VN968_28135"
     CDS             complement(1977..3005)
                     /gene="holA"
                     /locus_tag="VN968_28135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HXL33139.1"
                     /translation="MVALRGKDVDTFLARPDPGRPIILLYGPDSGLVRERAEALLASA
                     VDDPNDPFSLVRLDGDELSAEPSRLVDEAMTVPLFGGRRAIRVRAGSRSFASGVDTLA
                     DSPLMDCRIVIEAGELRPESPLRKACERAKTAVAIGCYPDGERDLAKLIDDELRVSSL
                     KIAPDARAALMALLGGDRQASRNELRKLTLYAHGGSEVTLEDVMAVVADASELKIDPI
                     VDGAFAGRPDLVETEFTKAMIAGTYPGMIISAAQRQAAWLHKSALAVAEGTPLSTLLD
                     SGFPRLHFSRKGNVETALRNFNAARLVLIIEQLATAALEVRKQAALASVIAQRTLLSI
                     AVNARRRG"
     gene            complement(3121..3672)
                     /gene="lptE"
                     /locus_tag="VN968_28140"
     CDS             complement(3121..3672)
                     /gene="lptE"
                     /locus_tag="VN968_28140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HXL33140.1"
                     /translation="MSLASIRIAARLLVVAGLAALTAGCFQPMYAEHTDGTPGLREKL
                     MGVEVPPVDKPNASQEARIQVEIRNALAFKLYGTATGMPPTHRLILKFTTSRSSLMID
                     PATALPSSENFGIDAQYNLIEIASNKSVMTGATFSRVSYDIPGQLQRFARSRAFRDAE
                     DRAAQEIAERIQTRLASFFVAGT"
     gene            complement(3659..6283)
                     /gene="leuS"
                     /locus_tag="VN968_28145"
     CDS             complement(3659..6283)
                     /gene="leuS"
                     /locus_tag="VN968_28145"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HXL33141.1"
                     /translation="MTSERYNARETEPRWQRQWDERAIFASKNDDPRPKYYVLEMFPY
                     PSGRIHIGHVRNYTLGDVLARFMRAKGFNVLHPMGWDAFGLPAENAAIERKVAPKAWT
                     YENIAAMKKQLRSIGLSLDWSREFATCDPSYYKHQQKMFLDFLRAGLAEREKRKINWD
                     PVDMTVLANEQVIDGRGWRSGAVVEQREMNQWVFKITKYAQELLDALDTLDRWPDKVR
                     LMQRNWIGRSEGMLVRFALDPATTPAGETELKIFTTRHDTLFGAKFMAIAPDHPLAQA
                     AAAKNPKLVEFIAEAKRIGTAQEIIDTQEKQGFDTGIKAVHPFDENWKLPVYVANFVL
                     MEYGTGAIFGCPAHDQRDLDFVNKYGLGNTPVVCPEGQDPKTFVITDTAYDGDGRMIN
                     SRFLDGMTIEQAKEEVAKRLEGAIRGNMPVGERQVNFRLRDWGISRQRYWGCPIPVIH
                     CPTCDVVPVPDKDLPVTLPEDATFDRPGNALDHHPTWKHVACPKCGAKALRETDTMDT
                     FVDSSWYFARFTDPWNENAPTTPAVADRLMPVDQYIGGVEHAILHLLYSRFFIRAMKA
                     TGHIGMDEPFAGMFTQGMVVHETYTKADGSYVTPAEVKVETGGNGRRASLLLTGEEVG
                     IGAIEKMSKSKRNTVDPDDIIMTYGADVARWFMLSDSPPDRDVIWSDERVQGASRFVQ
                     RLWRLVNESGEIAKAAPSARPAAFGSDALVLRKAAHGALDKVSTGIEKLHFNVCLAYI
                     REFSNTLGEVLGREGQPPPDLAWAVREAAVILVQLVAPMMPHLAEECWTVLGQSGLIS
                     EAHWPQIERDLLVEDTVTLVVQVNGKKRGEVTVARNAQNPEIEAAVLALDAVKQALSG
                     KPVRKVIIVPMRIVNVVG"
     gene            6646..7632
                     /locus_tag="VN968_28150"
     CDS             6646..7632
                     /locus_tag="VN968_28150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXL33142.1"
                     /translation="MSQQRVPPTPNNFHTWFKYSLGTSPELKRAIDILVGNKRKFDAT
                     TNRDLFAAYVGSHTADDTDLNNVSQQLHSVVASAKHYLKVAIADNRSQIRAISDVADQ
                     SEVEVDPKSLIENLLNELAQAVTRATKLEARFVESTRELDTIRDSLNKSEERAKTDTL
                     TDLPNRRALDEFFRKAQIAAMEQGEPLGVLLIDIDHFKAFNDNFGHGVGDQVLRLMAN
                     VLRERLRETDLPARYGGEELIAVLPGADLATCVAVAERIRRLISESRITRRSTGEILP
                     SITVSIGVGQFQFGESMADLIERCDRALYLAKRTGRDRVVTENELDRELKAS"
     gene            7942..8931
                     /locus_tag="VN968_28155"
     CDS             7942..8931
                     /locus_tag="VN968_28155"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXL33143.1"
                     /translation="MSQHRIPPTPNNFHTWFKYSLGTSSGLKRAIDILVGNKRKFDAT
                     TNHDLFAAYVGPQTAADDTDLNNVSQQLHSVMASAKQFLTVAIADNRSQIRAISDVAD
                     QSEVEVDPKSLIESLMHELAKAATRATKLETSFVESTRELDTIRDSLNKSEERARTDT
                     LTGLPNRRALDEFFRKAQIAAMEQGEPLGVLLIDIDHFKAFNDNFGHGVGDQVLRLMS
                     NVLRERLRDIDLPARYGGEELIAVLPGADLENCEAVAERIRRAISECRITRRSTGETL
                     PSITVSIGVGQFQFGESMADLIERCDRALYLAKRTGRDRVVTENELDRELKAS"
     gene            complement(8928..10625)
                     /locus_tag="VN968_28160"
     CDS             complement(8928..10625)
                     /locus_tag="VN968_28160"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXL33144.1"
                     /translation="MSRVTFNRKRVKLKKLLGIRARLALLALILVAPLMLERARSLEN
                     ARSKQIALASQEFSSLAQRSAEAQREMISSVETVLKSAAYIRASAGGVDRSCDILRAS
                     LPVNLPWIRTLLIVGGDGRVQCSTNDIFVGVDLSDRAYLKRARESHEIVFSDFLFAKL
                     TNKPVVMAAYPVFAINNDMDSIILAGVNLDWMSKIMSNLGGRPGVSAVLIDSKGTVLA
                     APADQASVIGQSLDAVPLLSAVAAKAIDSDQAEGTLSFVAVDGSRRAVSFARIAGTGS
                     RLIVSIDETKVSAAIDREIRTAYLQLGFVCLFVLLGALIAAEKLIIRPIEMMAAMAKR
                     FGQGDWSVRAARGRLPSEFIPLARAFNAMAAQLAERERELVATNDRLTVIASIDMLSG
                     LANRRGFQSRLDFEWMKARQYDAVLSLLMIDVDHFKLFNDTYGHPEGDVCLSRLGETL
                     AGIAAETMGFAGRYGGEEFCLLLPNTDAVRALEIGETVRAAVQALAMPHATSLYQTVT
                     VSVGVACTKPNDAQRPGDLIEAADAALYAAKHRGRNAVVEHGFVRVTDDAGTVALAG"
     gene            10733..11422
                     /locus_tag="VN968_28165"
     CDS             10733..11422
                     /locus_tag="VN968_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HXL33145.1"
                     /translation="MTSETPLPITPHLPNGLLSVEQDIARACQQARRDRSSVTLIAVS
                     KTFDAGAISPVIEARQRVFGENRVQEAKTKWPGLISAYPGLALHLIGPLQSNKAREAV
                     ALFDAIHSVDRPSICEALAKEIFSQKRAPQLFVQLNTGEEPQKAGVAPAEADGFIASC
                     REKYGLAISGLMCIPPVDDAPAPHFALTAKIAARNGLKNLSMGMSADFAIAIQFGATH
                     VRVGSAIFGKR"
     gene            complement(11439..12224)
                     /locus_tag="VN968_28170"
     CDS             complement(11439..12224)
                     /locus_tag="VN968_28170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXL33146.1"
                     /translation="MLGQDQIAAASQTLHDHWRAGTKLHGLEASLRPRDRAEGYAIQA
                     RIENYTTKTLFGWKIAATSEAGQRHINVDGPMAGRILPETVIPDGGTASMAGNEMRVA
                     EPEFAFRMHADLPPRPSAYTVREVVDAVETLHPAIEIPDSRFEDFVNAGAAQIIADNA
                     CAHLFVLGAPATSNWRALDLVEERPVITLRDKQFTGHGKNVLGDPRVALTWLANELRQ
                     LGVTLRAGQVVTTGTCHPPLPIQSGDVFEADFGLIGKVSVAFK"
     gene            complement(12250..12780)
                     /locus_tag="VN968_28175"
     CDS             complement(12250..12780)
                     /locus_tag="VN968_28175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXL33147.1"
                     /translation="MRDRPLSAVRIRAAAGDPRRGWLTADRQAIPVALGRGGIRANKR
                     EGDGGTPRGTFRPRQLWWRADRHPRPCTFLPIRAIRRQDAWCEDPASRHYNQPVRLER
                     ESGGDRLTRHDHLYDFIVEIDHNSSPRIAGRGSAVFLHLARDNLAPTAGCVSMTKSAM
                     LRLLRRLGPETRIVIG"
     gene            13044..>13663
                     /locus_tag="VN968_28180"
     CDS             13044..>13663
                     /locus_tag="VN968_28180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXL33148.1"
                     /translation="MANARKILIVDDDTDLRDTLVEQLSLHDEFEASAVDTGAKGASA
                     AKADAPDLVLMDVGLPDTDGREVVRSLRKGGFKAPIIMLTGHDTDSDTILGLESGAND
                     YVAKPFRFAVLLARIRAQLRQHEASEDAVFSVGPYSFRPGSKMLTGANARKVRLTEKE
                     TAILRFLYRAGQLPVSRETLLQEVWGYNSGVTTHTLETHIYRLRQK"
BASE COUNT         2646 a         4274 c         4204 g         2539 t
ORIGIN      
        1 gactcgatcc tggtgccgtt gcagtgtgag ttcttcgcgc tggagggtct gtcgcaattg
       61 ctgcagacgg tggagcaggt gcgctcgacg ctcaatccca gcctgacgat ccacggcatc
      121 gtgctgacca tgttcgactc ccgcaacaac ctgtcgaacc aggtcgtcgc cgatgtcagg
      181 cagttcatgg gcaacaaggt ctacaacacc atgatcccgc gcaacgtccg catctccgag
      241 gcgccgtcct acggcaagcc ggtgctggtg tacgatctca aatgcgtcgg cagcgaagcc
      301 tacctcaagc tcgcaacgga agtgatccag cgcgagcgcg acctgcgccc gcattgatcc
      361 acgctcgtca ttccggacaa tccgcaccgg cggattgatc cggaatcccg ggatgatgac
      421 gtagggctct ctcggaccta aacattgaga ttccgggttc gctcgtttca ctcgcgcccc
      481 ggaatgacgg cggtgaaacc gggagcactt aagttgaatc caagggagct ggcgatggtc
      541 gacgatgcgc gttcgcgatt gggccgcggt cttgcaagcc tgatcggaga tgtcggcggc
      601 gaagccaagc atcaggatcg gccgcgcacc cagcgcaagg tgccgatcga attcctcaag
      661 cccaatccgc gcaatccgcg ccgggatttt tccgatgccg agctcggcga actcgccgac
      721 tcgatcaggc agcacggggt gatccagccg atcgtggtgc gtccggtcaa gggctcgcag
      781 gaccgtttcg agatcatcgc cggcgagcgc cgctggcgcg cgtcgcaaat cgtcggcctg
      841 cacgaggtgc cgatcgtgcc ggtcgatgtc agcgacagcg acgcgctcga gatcgcgatc
      901 attgaaaacg tgcagcgcga agacctcaac ggtatggaag aggcgcaggg ctatcacgcc
      961 ctcgccaacg aattcaaacg cagccaggac gagattgcca agatcgtcgg caagagccgc
     1021 agccacgtcg ccaacatgat gcggctgacc aaattgccgg ccgaggtgca ggcctacatc
     1081 gcgttgggtc agctctcggc cggccacgcc cgcgcgctga tcggcgtgcc cgacccggcg
     1141 gccgccgcca agcgtatcgt cgaggaaggc ctcaatgttc gtcaggccga ggcgctggcg
     1201 catgtcgagg gcgtgcccga acgcaagccg cagaaggcgc gcggcggcaa gccgaaggat
     1261 cccaatacgg tcgcgctgga gaagcgggtc agcgacgcgc ttgggctcgc ggtcaacgtc
     1321 gaccaccgcg gaaccggcgg caccgtgcac gtccgttacg gagatctcga ccagctcgac
     1381 gagattgtcc gccggctgga acagggaagc tgactttctt acctcgcctc gctctgcgca
     1441 gggagaggtc gccgcgctct tcgcgcggag gggtcaggca ctgcgccaac ttcatcgtca
     1501 gcattcgtgg gtcggagagc ccctcacccc gaccccgcaa gtgagggcca gggagaagga
     1561 gcgtttgccg caatcatccc gcgcgtcgct tcagcgaaat tcggcgacga ccttgatctc
     1621 gccgacgatc gcctcattga aggcaaccag atcttcagca ggaatccagt attccaagtg
     1681 agccgagcca ccagcttttt ggattttgta tttgtccagg aacgtgcgct gaacttcaaa
     1741 ccgagtgacg aagccggagc cactggcggg gacgttccag tcgcgggcaa tcttcgcggc
     1801 atagtcctcc gacagcacag gatagaagat cggctgatcg ggaagccgcg gcgggaaggc
     1861 ttgcatgtca gccttccgga tcaattccag ttcgcgtggt ccgaccggac gccagagagt
     1921 tacgacatcc cggttctctt cagtgcgtgt gaacggcgcc ggctcgtcgg gcatgctcac
     1981 ccccgccgcc tggcgttcac cgcgatcgac agcagggtcc gctgcgcgat caccgacgcc
     2041 agcgccgcct gcttgcgcac ctcgagcgcc gccgtcgcga gttgctcgat gatcagcacc
     2101 agccgcgccg cattgaaatt gcgcagcgcg gtttcgacgt tgcccttccg tgaaaaatgc
     2161 agccgcggaa agccgctgtc gagcaacgtc gatagcgggg ttccctcggc aaccgcgaga
     2221 gccgatttat gcagccaggc cgcctggcgc tgcgcggcgg agatgatcat gcctggatag
     2281 gtgccggcga tcatggcctt ggtaaattcg gtttcgacca gatccggcct tccggcaaac
     2341 gcgccgtcca cgatcggatc gatcttcagc tcggaagcgt cggcgaccac cgccatcacg
     2401 tcctcgagcg tgacctcgct tccgccgtgc gcatagagcg tgagcttgcg taattcgttg
     2461 cgggaggcct ggcggtcgcc gccgagcagc gccatcagcg ccgcgcgcgc atccggcgcg
     2521 atcttcaaac tggagacccg cagctcatcg tcgatcaatt tcgccagatc gcgttcgccg
     2581 tcgggatagc agccgatcgc caccgcggtc ttggcgcgct cgcaggcttt gcgcagcggc
     2641 gattccggcc gcagctcacc ggcctcgatc acgatgcggc aatccatcag cggggagtcc
     2701 gcgagcgtgt cgacgccgct ggcgaagctt cgcgagcccg cccggacccg gatggcgcgg
     2761 cggccgccga acagtggcac cgtcatggct tcatcgacca gccgcgacgg ctcggccgag
     2821 agttcgtcgc cgtccagcct cacaagcgaa aatgggtcgt tgggatcgtc gaccgcggag
     2881 gccagcagcg cctcggcgcg ttcgcgcacg aggcccgaat cgggaccata gagcaggatg
     2941 atcggacgcc ccggatcggg ccgggcgaga aacgtgtcga cgtcttttcc gcgaagcgcg
     3001 accacggtca gactttcgtt gtcattccgg gacacccgcc ttcgcgtgga cccggaatcc
     3061 agatgtaatt gatcgagatt ccgggttcgc gcttcgcgcg cccggcgaat gacggaagga
     3121 tcaggtgccg gcgacgaaga acgacgccag ccgggtctgg attctttcgg cgatctcctg
     3181 ggcggcgcga tcctcggcat cgcggaaggc gcgcgagcgg gcgaagcgct gcagctggcc
     3241 cgggatgtca taggacacgc gtgagaatgt ggctccggtc atgaccgatt tattcgaggc
     3301 gatttcgatc agattgtact gcgcgtcgat gccgaagttt tcgctggaag gcagcgcggt
     3361 agcaggatcg atcatcagcg acgagcggct ggtggtgaac ttcagtatca gccggtgggt
     3421 gggcggcatg ccggtggcag tgccgtaaag cttgaacgcc agcgcattgc ggatttccac
     3481 ctggatccgc gcttcttgcg aggcgttagg cttgtccacc ggcgggactt ccacgcccat
     3541 cagcttttcg cgcaggccgg gggtgccgtc ggtatgctcg gcgtacatcg gctggaagca
     3601 gccggcggtc agcgccgcca atccggccac gaccagaagc cgagcggcga tacggatgct
     3661 agccaacgac attcacgatc ctcatcggaa caataatgac cttgcggacg ggcttgccgc
     3721 tgagcgcctg ttttaccgca tcgagggcca aaacggcagc ctcaatttcc ggattttgcg
     3781 cattgcgggc cactgtgact tcgccgcgtt tcttgccgtt gacctgaacc accagcgtca
     3841 cggtgtcttc aaccagcaaa tcgcgttcga tttggggcca gtgggcctcc gaaatcaggc
     3901 ccgattgccc cagcacggtc cagcactctt ccgccagatg cggcatcatc ggcgcgacca
     3961 attgaaccag gatgaccgcg gcctcgcgca cggcccaggc gagatccggc ggcggctgcc
     4021 cctcgcggcc cagcacctcg cccagcgtat tggaaaattc ccggatgtag gcgaggcaga
     4081 cgttgaaatg cagcttttcg atgccggtgg aaaccttgtc gagcgcccca tgcgccgcct
     4141 tgcggaggac aagcgcgtcg gaaccaaagg ctgccggccg ggccgatggc gcagccttgg
     4201 cgatctcgcc tgactcgttg accagccgcc acagccgctg cacgaaccgc gaggcgccct
     4261 gcacccgctc gtcgctccag atcacgtcgc gatcgggggg cgaatccgac agcatgaacc
     4321 agcgcgccac gtcggcgcca taggtcatga tgatgtcgtc ggggtcgacg gtgttgcgct
     4381 tcgacttcga catcttctcg atcgcgccga tgccgacctc ctcgccggtc aggagcagac
     4441 tggcgcggcg gccgttgccg ccagtctcga ccttgacctc ggccggcgtg acatagctgc
     4501 catcggcctt ggtgtaggtc tcgtgcacca ccatgccctg ggtgaacatg ccggcgaacg
     4561 gctcgtccat gccgatgtgg ccggtggcct tcatggcgcg gatgaagaag cggctgtaga
     4621 gcagatgcag aatcgcgtgc tcgacgccgc cgatatactg gtcgaccggc atcagccggt
     4681 ccgccaccgc gggcgtggtc ggcgcgttct cattccacgg atcggtgaag cgcgcgaagt
     4741 accacgacga gtcgacgaag gtgtccatgg tgtcggtttc gcgcagcgcc ttagccccgc
     4801 atttcgggca ggcgacgtgc ttccaggtcg gatgatggtc gagcgcgttg ccgggcctgt
     4861 cgaacgtcgc atcctccggc agcgtcaccg gcaggtcctt gtcgggcacc ggcacgacat
     4921 cgcatgtcgg gcagtggatt accggaatcg gacagcccca atagcgttgg cgcgagatgc
     4981 cccagtcgcg caggcgaaaa ttcacctgcc gctcgccgac cggcatgttg ccgcggatgg
     5041 cgccttccaa tcgcttcgcc acctcttcct tggcctgctc gatggtcatg ccgtcaagga
     5101 agcgcgaatt gatcatgcgg ccgtcgccgt cataggcagt gtccgtgatg acgaaggtct
     5161 ttgggtcctg gccttcggga cagaccaccg gcgtattgcc caaaccgtat ttgttgacaa
     5221 agtcgaggtc gcgctggtcg tgcgcggggc agccgaagat cgcgccggtg ccgtattcca
     5281 tcagcacgaa attggcgacg tagaccggca gcttccagtt ttcgtcgaac ggatggaccg
     5341 ccttgatgcc ggtatcaaag ccctgcttct cctgggtatc gataatctcc tgcgcggtgc
     5401 cgatgcgttt tgcttccgcg ataaattcca cgagttttgg atttttcgcg gccgcggcct
     5461 gggccagcgg atgatccggt gcgatcgcca tgaacttcgc cccgaacaag gtgtcgtgcc
     5521 gggtggtgaa gatcttcagt tcggtctcgc cggccggcgt cgtggccggg tcgagcgcga
     5581 accgcaccag cataccctcg gaccgcccga tccagttgcg ctgcatcagc cgcactttgt
     5641 cgggccagcg atccagcgta tcgagcgcgt ccaagagctc ctgcgcatat ttcgtgatct
     5701 tgaagaccca ctggttcatc tcgcgctgct cgacgacggc gccggaacgc cagccgcggc
     5761 cgtcgatcac ctgctcattg gcgagcacgg tcatgtcgac cggatcccag ttgatcttgc
     5821 gcttctcgcg ctcggccaac cccgcgcgca gaaaatccag gaacatcttc tgctgatgct
     5881 tgtaataaga gggatcgcag gtcgcgaatt cccgtgacca gtccagcgac agcccgatcg
     5941 accgcagctg ctttttcatc gccgcgatat tctcgtaggt ccaggccttg ggcgcgacct
     6001 tgcgctcgat cgcggcattt tccgccggca gtccgaacgc atcccagccc atcggatgca
     6061 gcacgttgaa acccttggcc cgcatgaagc gcgccagcac gtcgcccagc gtgtagttgc
     6121 ggacatgccc gatatggatg cgcccggacg gataggggaa catctcgagc acgtaatatt
     6181 tcggccgcgg gtcgtcgttc ttggaggcga agatcgccct ttcgtcccac tggcgttgcc
     6241 agcgcggctc ggtttcgcgg gcgttgtagc gttcggaggt catggaattg taagggcttt
     6301 ttgatggatt cgcggcggtc ttttgggacg gcggactagg ccataaaaga ccgcgatggg
     6361 tcaatgggtt gtaggcaagg gaaagaccgc cgtttgcgcg acctcggggc gaaaattcgg
     6421 aaaaactaaa ctcaattttc acgcaaaata gctgcagtac ttggctatta agccagtccg
     6481 aacatttgcc tgctttggat tgagccacga ttaacggttc catgggacgg tgcggccgaa
     6541 gaatttccgc accagcaggg atcaccttta atcgtgacag gcgacgcgtt tgacgagctt
     6601 gacttcgagt atgcgacgga agtcgcggaa aaagccgtgc gatcgatgtc gcaacagcgc
     6661 gtaccaccga cgccgaacaa ttttcacacc tggttcaaat attccctcgg tacctcgccc
     6721 gaactcaaac gggctatcga tattctggtc ggcaacaagc gaaagtttga cgccaccacc
     6781 aaccgcgacc tgttcgcggc ctatgttgga tcgcacaccg ccgacgacac agacctcaac
     6841 aacgtctccc agcagctgca ttcggtcgtg gcctccgcaa agcactatct caaggtggcc
     6901 atcgccgata accgttcgca aatacgcgcc atcagcgatg tcgccgacca gagcgaagtc
     6961 gaagtcgatc caaaatcgct catcgagaat ctgctgaacg agctggccca agccgtcacc
     7021 cgtgcgacga aactcgaggc gcgtttcgtc gagtcgacgc gagagctgga cacgatccgc
     7081 gactccctga acaaatcgga agagcgcgcc aagaccgaca cgctcacgga tctgccaaac
     7141 cgccgcgccc tcgatgagtt ttttcgcaag gcccagatcg cggcgatgga acaaggcgag
     7201 ccgctcggcg tcctgctgat cgacatcgat catttcaagg cgttcaacga caattttggg
     7261 cacggcgtcg gcgaccaggt gcttcggttg atggcgaacg tgttgcggga gcggcttcgg
     7321 gaaaccgatc tgccggctcg atatggcggg gaagaactca tcgccgtgct gccaggggcc
     7381 gacctcgcca cctgcgtggc ggttgccgag cgcatccgcc gcttgatctc ggaatcccgc
     7441 atcacccgcc gctcgaccgg ggaaattctt cccagcatca cggtttccat cggagtcgga
     7501 cagtttcagt tcggcgagtc gatggcggat ttgatcgagc gatgcgatcg cgcgctatat
     7561 ctggccaaga gaaccggacg cgaccgggtc gtgaccgaga acgaactcga tcgtgagctg
     7621 aaggcgagct agccagccag cgccactgta ccggcgccat ccgttatccg tcatccctgc
     7681 aaagccacct cgccgacctc caacgcgccg cccgcgttgg tattcggcgg cagcagacaa
     7741 gttccttacc gccataacgc acggcaaagg ccgtagaatc actgaactct caatgaggtg
     7801 cagttaacgc atacgtggga cgctgggtcg acaacgggaa ttggctcaca acgggagatc
     7861 gtttaaatcg tgacagggca cgcgcttgac gagcttgatt tcgagtacgc gacggaagtc
     7921 gcggaaaaag ccgtgcgatc gatgtcgcaa catcgcatac cgccgacgcc gaacaatttt
     7981 cacacctggt tcaaatattc cctcgggacc tcatccggtc tcaaacgggc catcgatatt
     8041 ctggtcggca acaaacgaaa gtttgacgcc accaccaatc atgacctgtt cgcggcctat
     8101 gtcggaccgc aaaccgccgc cgacgacacc gacctcaaca acgtttccca gcagctccat
     8161 tcggtgatgg cctctgcaaa gcagtttttg acggtggcca tcgccgataa ccgctcgcaa
     8221 atacgcgcca tcagcgatgt cgccgaccag agcgaagtcg aggtcgatcc aaaatcgctt
     8281 atcgagagtt tgatgcacga gctggccaaa gccgctaccc gtgcgacgaa actggagacg
     8341 agctttgtcg agtcgacgcg agagctggac acgatccgcg actccctgaa caaatcggaa
     8401 gagcgcgcca ggaccgacac gctcaccggc ctgccaaacc gccgtgcgct cgatgagttt
     8461 tttcgcaagg cccagatcgc agcgatggaa caaggcgagc cgctcggcgt actgctgatc
     8521 gacatcgatc atttcaaggc gttcaacgat aattttggcc acggcgtcgg cgaccaggtg
     8581 cttcggttga tgtcgaacgt gttgcgggag cggcttcggg atatcgatct gccggctcga
     8641 tatggcgggg aagagctcat cgccgtgctc ccgggtgccg atctcgaaaa ttgcgaggcg
     8701 gttgccgagc gcatccgccg cgcaatctcg gagtgccgca tcacccgccg ctcgaccggg
     8761 gaaactctcc ccagcatcac ggtttccatc ggggttggac agtttcagtt cggcgagtcg
     8821 atggcggatt tgatcgagcg atgcgatcgc gcgctatatc tggccaagag aaccggacgc
     8881 gaccgggtcg tgaccgagaa cgaactcgat cgtgagctga aggcgagcta acccgccagc
     8941 gccactgtac cggcgtcatc cgtcacccgc acaaagccgt gttcgaccac ggcgttgcgg
     9001 ccacggtgtt tggcggcata gagcgcggcg tcggcggcct cgatcaggtc gcccggccgc
     9061 tgggcgtcgt tcggtttggt gcaggcgacc ccgacgctga cggtgacggt ttggtagagg
     9121 ctggtggcgt ggggcatcgc gagggcctgg accgccgcgc gcaccgtctc gccgatctcc
     9181 agcgcgcgga ctgcatcggt attcggcagc agcagacaga attcctcgcc gccatagcgc
     9241 ccggcaaaac ccatggtctc ggcggcaatt cccgccagcg tctcgccgag tctggagagg
     9301 cagacgtcgc cttcgggatg gccataggtg tcgttgaaca gcttgaaatg atcgacgtcg
     9361 atcatcagca gcgacagcac ggcgtcatat tgccgggcct tcatccattc gaagtcgagc
     9421 cggctctgga agccgcgccg gttggcaagg ccggagagca tgtcgattga ggcgatcacg
     9481 gtcagccggt cgttggtggc gaccagctcg cgttcgcgtt cggcaagctg tgccgccatc
     9541 gcgttgaatg cgcgcgccag cgggataaat tccgacggca agcggccgcg tgccgcgcgc
     9601 accgaccagt cgccctggcc gaagcgtttg gccatcgctg ccatcatctc gatcggccgg
     9661 atgatgagct tttccgcggc gatcaacgcg ccgagcagca cgaacaggca gacgaacccc
     9721 agctgcagat aggcggtgcg aatctcgcgg tcgatagcgg cggaaacctt ggtctcgtcg
     9781 atgctgacga tcaggcgtga cccggtgccg gcaatgcgcg caaaactgac cgcgcgcctc
     9841 gagccatcca cggcgacgaa cgaaagcgtt ccttccgcct gatccgaatc gatggccttt
     9901 gcggcgacgg cggatagcaa tggtactgca tccaacgact ggccgatcac actggcctga
     9961 tcggccggcg ccgccagcac ggttcccttg ctatcgatca gaaccgctga aacaccgggc
    10021 cggccgccga gattgctcat gattttcgac atccagtcga gattgacgcc ggcaagaata
    10081 attgaatcca tatcgttatt gatggcgaag accggataag ccgccatcac gactggcttg
    10141 ttggtaagct tggcgaacag aaagtcgctg aagacaatct cgtggctctc gcgcgccctc
    10201 ttgaggtagg cccggtcact gagatcgaca ccgacaaaga tgtcgtttgt cgagcattga
    10261 acgcgtccgt ccccgccaac aatcagcaaa gtgcgaatcc acggcagatt gaccggtagg
    10321 ctggcgcgca ggatgtcgca actacgatcc acgccgccag ccgaggcgcg gatataggcc
    10381 gccgatttca gcactgtctc gaccgaggag atcatttcgc gctgcgcctc agcgctacgc
    10441 tgcgcgagac ttgagaattc ctgggaggct agcgcgatct gcttgctgcg ggcgttctcg
    10501 agcgaacgcg cgcgttccag catcaagggt gccaccagga tcagcgccag cagcgccagt
    10561 cgcgcgcgaa ttccaagaag cttcttgagt ttcacccgtt tgcggttgaa agtaacgcgt
    10621 gacatcgtcg tcccccggcc ccacatgcag ggtagagaga aggcttcaag aagcccttcc
    10681 cgaatttggt aaaattcgaa cgaccccgta aacttacgac agatgaaatg tcatgacatc
    10741 cgaaacccca ttgccgataa ctccgcattt accaaacggg ctgctctcgg tggagcaaga
    10801 catcgcgcgc gcgtgccagc aggcgcgccg ggatcgctcg tcggtgacgc tgatcgcggt
    10861 atccaagacc ttcgatgccg gcgccatttc acctgtgatc gaggccagac agcgcgtatt
    10921 cggcgaaaat cgcgtgcagg aagccaagac gaagtggcca gggttaatat cggcttaccc
    10981 cggtctcgcc ttgcacctga tcgggccgct gcaatccaac aaggccaggg aagccgtggc
    11041 gctgttcgac gcgatccatt cggtcgaccg tccgagcatt tgcgaagcgt tagccaaaga
    11101 aatcttttct caaaaacgcg cgccgcaatt gttcgtccag ctcaataccg gcgaggagcc
    11161 gcagaaggcg ggcgtcgcgc cggccgaggc ggacggcttc attgccagct gccgcgaaaa
    11221 atacgggttg gcaatttccg gcctgatgtg cattccgccg gtcgacgatg cgccggcgcc
    11281 gcatttcgcg ctgaccgcga agatcgcggc gcgtaacggg ctgaaaaacc tctcgatggg
    11341 catgagcgcc gattttgcga ttgcgatcca gttcggcgcg acgcatgtgc gcgtcggctc
    11401 ggcgattttc gggaagcggt gacgcttcgt cattgcgcct atttgaacgc gaccgacact
    11461 tttccgatca agccgaaatc cgcctcgaaa acatcgcccg actgaatcgg cagcggcggg
    11521 tggcaggtgc ctgtggtcac gacctggccc gctctcagcg tcacgccaag ctggcgcaat
    11581 tcattggcga gccacgtcaa ggcaaccctg gggtcgccga gcacgttctt gccgtgaccg
    11641 gtgaattgct tgtcccgcag cgtgatgacc ggccgctcct cgacgagatc gagcgcgcgc
    11701 cagttcgacg tcgctggcgc accaagcacg aacaggtgcg cgcatgcatt gtccgcgatg
    11761 atttgcgcgg ctccggcgtt gacgaaatcc tcaaaacgcg aatcgggaat ctcgatcgcc
    11821 ggatgaagtg tctcgaccgc atcgacgacc tctcgcacgg tgtaggctga aggtcgcggt
    11881 ggcaagtccg catgcatgcg aaaggcgaat tcgggctcgg cgacgcgcat ttcatttccg
    11941 gccatggaag cggtaccgcc atccggaatg accgtctcgg gcaggatgcg tcccgccatc
    12001 gggccatcga cattgatatg cctttgcccg gcttcgctgg tggctgcgat tttccagccg
    12061 aacagggttt tggtcgtgta attttcgatt ctcgcctgga tcgcgtaacc ttcggcgcgg
    12121 tcgcgcggcc gcagcgaggc ttcgagacca tgaagctttg tgcccgcacg ccagtgatcg
    12181 tgcagggtct gtgacgcagc cgctatctga tcttggccga gcattgtttt tggtatcccg
    12241 gaatggcgtt catccaatca cgattctggt ctcgggaccg agtcgccgca gtaaccgcag
    12301 catggcggat ttcgtcatcg agacgcatcc cgcggtcggc gcaagattgt cgcgcgccaa
    12361 gtgcaggaat accgcgctgc cgcggccggc aatgcgcggg gagctgttgt gatcgatctc
    12421 gacgatgaaa tcatacagat ggtcgtggcg cgtcagccga tcgccgccgc tctcgcgttc
    12481 cagccgcacc ggctggttgt aatgacggct cgccggatct tcgcaccagg cgtcttggcg
    12541 ccggatggcg cgaatcggca ggaatgtgca tggccgcggg tgacggtcgg cgcgccacca
    12601 taattgccgc ggccggaacg ttcccctcgg ggttccgcca tccccttccc gcttgttggc
    12661 cctgatgccg ccgcgcccga gtgcgaccgg tatggcctgc ctgtcggcgg taagccagcc
    12721 gcggcgcgga tccccggcgg cggcgcgaat cctgacggcg gaaagcggtc gatcacgcac
    12781 ggttctctga taagtgattg aaatgtcggg aattcccatg cgatacgcga atctctatgg
    12841 tttgacgcta gctctaaatg tttctatttc acgccattac aggacattca tccaaaaccc
    12901 cggcaattct ggagtagact gatgcaactt gtgaatctgt aacagcccct tacttcaata
    12961 tgaatcaaga ccaaccgacc gcgattttac cgaccaaaac tcgactgata gcgcttcgcg
    13021 cctgccccca gaggattgta cccatggcca atgcccgcaa gatcctgatc gtggatgacg
    13081 acaccgatct gcgcgatacg ctggtggagc aattgtcgct gcatgacgaa tttgaagcct
    13141 cggcggtcga taccggcgcc aagggtgcca gcgccgccaa ggccgatgct cccgatcttg
    13201 tgctgatgga tgtcggactg cccgataccg atggccgcga agttgtgcgc tccttacgca
    13261 agggcggctt caaggctccc atcatcatgc tcaccggaca cgataccgat tcggacacga
    13321 tcctcgggct cgaatccggc gccaacgact acgtcgccaa gccgttccgt ttcgccgtgc
    13381 tgctggcgcg gatccgggcg cagctccgcc agcatgaagc cagcgaagac gcggtgttct
    13441 ccgtcggccc ctacagtttt cgacccggat ccaagatgct gaccggcgcc aacgcccgta
    13501 aggtgcgcct gaccgaaaag gaaacggcga tcctgcggtt tctgtaccgg gccggccagc
    13561 tgccggtatc gcgcgagacg ctgctgcagg aagtatgggg ctataattca ggcgtcacca
    13621 cccacaccct ggaaacccac atttaccggc tgcgtcagaa gat
//
LOCUS       DATNOI010000707         3318 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1818808,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000707 DATNOI010000000
VERSION     DATNOI010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818808"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VN968_28185"
     CDS             complement(<1..366)
                     /locus_tag="VN968_28185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD regulator"
                     /protein_id="HXL33149.1"
                     /translation="MSDKLLTPIEIGLTAAIVAIEGNEPLILTASGSGGDELAGLPFG
                     PFDAVSHRTFEIGLRAWVEEQAGLRLGYVEQLYTFGDRGRHTQAGDTDAHVASIGYLA
                     LTRAADNASRAPGATFEPWY"
     gene            complement(386..892)
                     /locus_tag="VN968_28190"
     CDS             complement(386..892)
                     /locus_tag="VN968_28190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HXL33150.1"
                     /translation="MIHVCSLAALPDTVKATGASHVLTVMANVDQVQRPESVLPANHL
                     KISMDDITEQMDGFVAPSDSHIEQVLNFVRGWDRSAPLVVHCYAGISRSTASAFAAVC
                     MLNPHRDEISIARQIRAASPIASPNRLIVSLADRALGRDGRMLRALDEMGPGTMMVEG
                     RPFRIDLD"
     gene            complement(953..1570)
                     /locus_tag="VN968_28195"
     CDS             complement(953..1570)
                     /locus_tag="VN968_28195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HXL33151.1"
                     /translation="MTARKFSETATSTRVWQRMLSGRRLDLLDPSPLDIEIADIAHGL
                     ARVARWNGQTSGAHIFSVAQHTLLVEAVMREQIPRVDVRFRLAALLHDAPEYVIGDMI
                     SPFKAVLGGDYKAVEKRLLSAIHIRFGLPPVLADEITRQIKAADRGAAYLEATELAGF
                     AEAEAKRLFGRDPGLPPASVRDYLTPWTAAKAEKRFLARFKTLLP"
     gene            complement(1567..2193)
                     /locus_tag="VN968_28200"
     CDS             complement(1567..2193)
                     /locus_tag="VN968_28200"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HXL33152.1"
                     /translation="MTRSARLHADGLVRCPWPGEDPFYMAYHDTEWGVPEYDDRALYE
                     KLILDGFQAGLSWITILRKRDNFRRAFDDFEPAKIARYNAKKTDALMNDAGIVRNRAK
                     IEGTIASAKSYLKIMDDGPGFSKFLWDFVDGRPKVNQFKTTASVPASTPLSIKISKEL
                     ASRGFKFVGPTIVYAFMQATGMVNDHLVTCFCHEACAGKQRSTRLKVK"
     gene            complement(2190..3071)
                     /locus_tag="VN968_28205"
     CDS             complement(2190..3071)
                     /locus_tag="VN968_28205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HXL33153.1"
                     /translation="MKAAFLPDRGVVKVSGGDARDFLNGLVTTDVTLLRPSLGRFGAL
                     LTPQGKITVDFLITEAPSGHGGGFLIDCPRALAQGLADKLGFYKLRAKVAVENLSDSL
                     GVLAAWDGVPAVIPDLAFTDPRDAALGWRILVPAALAQKVADLVGADLVDSTAYEAHR
                     IASGVPRGGLDFMYGDAFPHETNMDRLHGVDFDKGCYVGQEVVSRMQHRGTARTRIVR
                     VILDGPSPEPGAAVLAGDKPVGTMGSTAGHNGLALIRVDRAADAHAAGMPLTSGGLAI
                     RVAEPDDLLIAPKQTVA"
BASE COUNT          612 a         1032 c         1070 g          604 t
ORIGIN      
        1 ataccagggc tcgaaggtcg cgccgggcgc gcgcgaggcg ttgtcggccg cgcgcgtcag
       61 cgcgaggtag ccgatcgacg ccacatgggc atcggtgtcg ccggcctggg tgtgccggcc
      121 gcgatcgccg aacgtataaa gttgctcgac atagcccagc cgcaatcccg cttgttcttc
      181 cacccaggcg cgcaggccta tctcgaaggt gcggtgcgag accgcgtcga acggcccgaa
      241 cggaagcccg gcgagctcgt cgccgccgct gccggacgcg gtgaggatca acggctcgtt
      301 gccctcgatc gcgacgatag cggcggtgag gccgatttcg atcggcgtca gaagcttgtc
      361 gctcatctgg cgccattcac cgacatcagt cgagatcgat gcggaacggc cggccttcga
      421 ccatcatggt gcccgggccc atttcgtcga gcgcgcgcag catccgccca tctcgcccca
      481 aagcccggtc cgccaggctc acgatcaacc ggttcggcga cgcgatcggg gaggcggcgc
      541 gaatctgtcg tgcgatcgag atttcgtcac gatgcggatt gagcatacac acggcggcga
      601 aagcgcttgc ggtcgagcgg ctgatgccgg cgtaacaatg gaccacgagc ggggcgctgc
      661 ggtcccagcc gcgcacgaaa ttcaggacct gctcgatatg cgaatccgaa ggcgcgacaa
      721 agccatccat ctgttcggta atgtcgtcca tcgatatttt caaatgattg gccggcagca
      781 ccgattccgg ccgctgcacc tgatcgacgt tggccatgac agtgaggacg tgactggctc
      841 cggtggcttt gacggtgtcg ggaagtgcgg cgagcgagca aacgtggatc atggcggtcc
      901 ttttgttcgt gcaattatag gcttgcccac gccgcggcga aagcgtcgat gttcatggaa
      961 gcagcgtctt gaaccgcgcc aggaaccgct tttcggcctt ggccgcggtc cagggcgtga
     1021 ggtaatcccg cacgctcgcc gggggcaggc caggatcgcg gccgaacagg cgcttggcct
     1081 cggcttcggc aaaacctgca agctcggtcg cctcgaggta tgccgcgccc ctgtcggcgg
     1141 ccttgatctg tcgcgttatc tcgtcggcca ataccggcgg caatccgaag cggatgtgga
     1201 tggccgaaag cagccgcttc tccacggcct tgtagtcgcc gcccagcacc gccttgaacg
     1261 gcgagatcat gtcgccgatg acatattccg gtgcatcgtg cagcagtgct gcgagccgga
     1321 agcggacatc gacacgcggg atctgctcgc gcatcacagc ctcgaccagc agcgtgtgct
     1381 gcgccaccga gaaaatatgc gcgccgcttg tctgcccgtt ccagcgcgca acgcgcgcca
     1441 ggccgtgggc gatgtcggcg atttcgatat ccagcggcga ggggtccaaa agatcaaggc
     1501 gccggcccga caacatccgc tgccagaccc gggtggaggt cgcagtctcg gaaaattttc
     1561 tggccgtcat ttgactttga ggcgggtgct gcgttgtttt ccggcgcagg cttcatggca
     1621 gaagcacgtc accagatggt cgttgaccat gccggtggcc tgcatgaagg cgtagacgat
     1681 ggtcgggccg acaaatttga atccgcgcga tgccagttcc tttgatatct tgatcgacag
     1741 cggcgttgac gccggcacgc tggcggtggt cttgaactga ttgaccttcg gcctgccgtc
     1801 gacaaaatcc cacaggaatt tcgagaagcc ggggccgtcg tccatgatct tcagatagga
     1861 ttttgcgctt gcgatcgtgc cctcgatctt ggcgcggttg cgaacgatgc cggcgtcgtt
     1921 catcagggca tcggtctttt tcgcgttgta gcgcgcgatc tttgccggct cgaaatcgtc
     1981 gaaggcgcgg cgaaagttgt cgcgcttgcg caggatcgtg atccacgaca gcccggcctg
     2041 gaagccgtcg aggatgagct tttcgtacag cgcgcggtcg tcgtactccg gcacacccca
     2101 ttcggtgtcg tgataggcca tatagaacgg atcttcgccc ggccacggac accgcaccag
     2161 gccatcggca tgcaggcgcg ccgacctcgt catgccacgg tctgtttcgg tgcgatcagc
     2221 agatcgtccg gttcggcaac gcggatggca aggccaccgg atgtcagcgg catcccggcg
     2281 gcatgcgcgt cggcggcacg atccaccctg atcagcgcga gaccattgtg gcctgccgtc
     2341 gaacccatgg tgccgaccgg cttgtcgccg gccaggacag ctgctccggg ttccggcgac
     2401 gggccatcga gaatgaccct tacaatccgg gtgcgcgcgg tgccgcgatg ctgcatccgc
     2461 gacaccacct cctgcccgac gtagcagccc ttgtcgaaat cgacgccgtg caggcggtcc
     2521 atattggttt cgtgcggaaa tgcgtcgcca tacatgaaat cgaggccgcc gcgcggaacg
     2581 ccggacgcga tgcgatgggc ctcataggct gtgctgtcga ccagatccgc gcccaccaga
     2641 tcggcgactt tttgcgcgag tgccgcaggc accagaatcc gccaaccaag cgcggcatcg
     2701 cgcggatcgg tgaacgccag atcgggtatc accgcgggaa cgccatccca tgccgcgagc
     2761 acaccgaggc tgtcggagag attttcgacc gcgaccttgg cgcgcaattt gtagaaacca
     2821 agcttgtcgg cgagaccttg cgcgagcgcg cgcgggcagt cgatcaggaa accgccgccg
     2881 tggcccgaag gcgcttcggt gatcaggaaa tccaccgtta ttttgccctg cggggtcagc
     2941 aaggccccga accggccgag gcttgggcga agcagcgtca cgtcggtggt gacaaggccg
     3001 ttgagaaagt cgcgggcgtc accgccgctg accttgacca cgccccggtc gggaagaaac
     3061 gctgctttca taaggagttc tctggcaaag ttctttttga agttcttggg gaaacgcaac
     3121 tgccggatat cacatggcaa cgtaagacgc taaggtattg cgaacaaggc ccgcgccaca
     3181 tccggttccg cgaaccgggc actgaggacc gatgaatcaa cgatttgata ccattctaag
     3241 atccggcacc gtggtcaatc aggacggcga gggcgtgcgc gacatcggcc tcgccggtgg
     3301 ccgcattgca gcaatcgg
//
LOCUS       DATNOI010000708         6880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_267254,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000708 DATNOI010000000
VERSION     DATNOI010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267254"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1374)
                     /gene="soxC"
                     /locus_tag="VN968_28210"
     CDS             complement(49..1374)
                     /gene="soxC"
                     /locus_tag="VN968_28210"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HXL33154.1"
                     /translation="MANARHRNAHGQPLEAAAGNGLIDRRALLGRGMVFAGAMGAGMG
                     PSSAAAGAEPLADDPWSMEVGDPMPLYQVPSKFETKVARTLSNPNHEPRNSHARTPHH
                     LLEGTTTPNGLHFAINHAGVPTIDPEKHRLVIHGLVKRPLEFTLETLSRYPMISRVSF
                     VECGGNSAPMFSPEPMQETVQALHGLASCAEWTGIKLSTLLEETGIDPSAKWFIAEGA
                     DSPRLSRSVPLKKALDDAMIALYQNGERIMPGNGYPMRLLLPGYEGNMNVKFLRRIKL
                     VDQPAMSMYEARTYSQILPNGKAYRFYFLQEVKSFITSPSHGMMLKGPGLYEISGIAY
                     SGTGRVEKVMVSADGGQSWAQAALQEPVLSKAFTRFRMPWRWDGGAATLQSRAWDEAG
                     NVQPTRAEFVAARGELTKPAPVLSFPNQHYNSITSWGVGSNGEVKHVYV"
     gene            complement(1547..2638)
                     /locus_tag="VN968_28215"
     CDS             complement(1547..2638)
                     /locus_tag="VN968_28215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXL33155.1"
                     /translation="MQSAVRAQSGTSQLHRSRIERPSAHGGTTQGRKPMKLSRRKLLH
                     LAAGAAVLPAFSGIARAQAYPTRPITLVAPFPAGGPVDTIARILAEHMRGSLGQPLLV
                     ENIAGAAGSLGVGRVARAAPDGYTLIVGQWSTHVANAAIYKLQYDTLADFAPIALLSN
                     NPGLIVGRKNLPANDLKELVAWLKANPETATQGTPGVGGFGHIGGVFLQNVTGTKYQF
                     VPYRGAAPMIQDLLAGQFDIAIDTPTTSIPQIRSGAIKGFAVMSKTRLAAVPDLPTAD
                     EAGVPGLYLLQWNAVWAPKGTPGDIVTRLNQAIVRAMADPAVRQRLVDLGQEIYPPEL
                     QTPEALAEFQKTELHKWGPIITAANIKVE"
     gene            complement(2968..3479)
                     /locus_tag="VN968_28220"
                     /pseudo
     CDS             complement(2968..3479)
                     /locus_tag="VN968_28220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
     gene            complement(3493..4320)
                     /locus_tag="VN968_28225"
     CDS             complement(3493..4320)
                     /locus_tag="VN968_28225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HXL33156.1"
                     /translation="MTAHVEVMELVARLKAAEETFVLATVVRTVSVTAAKAGAKAIIR
                     PDGTIVAGWIGGGCARGAVLRAAREALADGEPRMVSVQPENLLAELGVKPGENRDGVR
                     FAENMCPSKGTMDIFVEPVLPHPSLVILGASPVALSLAAQARQLGYHVTLAAPAADVA
                     SESDAHVVVDGFQPRYLNDARRFVVISTQGKGDEAALKASVGIDAAYHAFVGSRRKMA
                     ALRAKLIAEGIDSSALDRIKAPAGLDLGAITPEEIAMSILAEITTLRRCGQRTTTNV"
     gene            complement(4332..5540)
                     /locus_tag="VN968_28230"
     CDS             complement(4332..5540)
                     /locus_tag="VN968_28230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXL33157.1"
                     /translation="MTCCGTNPGYEELDEVSRLVATKLAAFLKTLRDNAFAVGLHEGR
                     DAAALMAAGYAEKPALLRSAFKHLFSARKSDWEKFDGLFDAFWLGKRVKSRSVTMGSA
                     KAANSPSLKSLQDSKSERTGSEAATDQIPSDDDAPQNRTGEGRMEGASRADNLAEIDF
                     RNMADPDQIDEAHAVAARLAKTMRTRLTRRDLARRRGYRLDLRRTIHNNISHGGVPIS
                     LVKRQHKEKPLRLVMLLDASGSMSMYTGVFLRFIHGVLDEFREAEAFLFHTRLAYVSD
                     AMKEKDAGRALDRLSIMAQGTGGGTKIGESLQTFNRWHAARVLHSRACVMIVSDGYET
                     GDAALLGREMAALAKRCRRIVWLNPMMAWEGYAPEAAGIKAALPHVDLYAPANTLKSL
                     TALEPYLAKL"
     gene            complement(5537..6409)
                     /locus_tag="VN968_28235"
     CDS             complement(5537..6409)
                     /locus_tag="VN968_28235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXL33158.1"
                     /translation="MRDREEIAERLAASGYIADAELATAISLMQLLRRPLLLEGEAGV
                     GKTEVAKALAAAHATELIRLQCYEGLDQSSALYEWNYQRQLLAIQAHRGGDADAIEDQ
                     IFSEKYLLERPLLAAIRRDKPPVLLIDEIDRADDEFEAFLLELLSDFQVSIPELGTIS
                     ATTIPHVVLTSNGTRELSDALRRRCLYHYVDYPDVDREARIIMARINGASASLSLQIA
                     RMVENVRKEELRKVPGVAETLDWAAALVGLEVRDLHDAPEVVHETLMCLLKTHEDKSR
                     VTREVTERLLGKVA"
     gene            complement(6412..>6880)
                     /locus_tag="VN968_28240"
     CDS             complement(6412..>6880)
                     /locus_tag="VN968_28240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXL33159.1"
                     /translation="MTYPFGAYICVMNIDVDTGVYKIRRFYALDDCGTRINPMIIEGQ
                     VHGGLTEAFAIAMGQEIRYDDEGNVVTGSFMDFFMPTAVETPHWETDFTVTPSPHHPI
                     GAKGVGESPNVGGVPAFSNAVNDAFSFLGSTHIQMPHDYWRNWRAAKNLGVFA"
BASE COUNT         1220 a         2194 c         2132 g         1334 t
ORIGIN      
        1 gcgccggtca tcatcgccag caataatgag gcggaaaaga tcgcgcatct agacgtaaac
       61 atgcttgacc tccccattgc ttccgacgcc ccaactggtg atcgagttgt agtgctgatt
      121 gggaaagctc aggaccggcg ccggtttcgt caattcgccg cgtgcggcca cgaattcggc
      181 gcgggtcggc tgcacattgc cggcttcatc ccacgcccgg ctctgcagcg tggccgcgcc
      241 gccgtcccag cgccatggca tgcggaaacg ggtgaaggcc ttgctcagga ccggttcttg
      301 aagtgccgct tgcgcccagc tttggccgcc gtcggcggag accattactt tctcgacccg
      361 gccggtgccg gaataggcga tgcccgaaat ctcgtagaga ccaggaccct tcaacatcat
      421 gccgtgcgaa gggctggtga tgaaggattt gacttcctgc aggaaataaa accggtacgc
      481 tttgccgttc ggcaggatct gcgagtaggt ccgcgcctca tacatgctca tggcgggctg
      541 gtccacgagc ttgatgcggc gcaggaactt gacgttcatg ttgccctcgt agccgggcag
      601 cagcaggcgc atcggatagc cgtttccagg catgatgcgc tcgccgttct ggtagagcgc
      661 gatcatcgcg tcatccaacg ccttcttcaa cggcacgctg cggctcaaac ggggcgagtc
      721 cgcgccctcg gcgatgaacc atttcgcgct gggatcgatg ccggtttcct caagcagggt
      781 cgaaagcttg attccggtcc actccgcgca ggaggcgagc ccgtgtagtg cctgcacggt
      841 ttcctgcatc ggctcgggag agaacatcgg ggcgctgttg ccgccgcatt cgacgaacga
      901 cacgcgcgag atcatgggat agcgcgacag tgtttcgagc gtgaactcca gcggccgctt
      961 caccagtcca tggatcacga gccggtgctt ctccggatcg atggtgggga cgccggcgtg
     1021 attgatggcg aaatgcagcc cattcggcgt ggtcgtaccc tcgaggagat ggtgcggcgt
     1081 gcgcgcatgt gaattacgcg gctcgtgatt gggattactc agcgtgcgcg caaccttcgt
     1141 ctcgaacttc gacggcacct ggtagagcgg cattgggtcg ccgacttcca tgctccatgg
     1201 atcatcggcg agcggttcgg cgcccgcggc cgcagatgac gggcccatgc ccgcgcccat
     1261 cgcgcccgcg aacaccatgc cgcgcccgag tagcgcgcga cggtcgatca gcccgtttcc
     1321 ggcagcggct tccaaaggtt gaccgtgcgc attgcgatgc ctggcattag ccatgctagc
     1381 ctcccttgag tacggacgtt acaggtcgtc ctttcttctc actcgctact gtagctcttt
     1441 gcgataaggc cgtctcctga tacatcgcgc aagacagatc tcggtaggac agcatgccat
     1501 cgtcaccatc gatgccatcg gctgtcagaa gaatgtcgag cgccggttac tcgaccttga
     1561 tatttgccgc cgtgatgatc gggccccatt tatggagctc ggttttctga aattccgcca
     1621 gcgcttcagg cgtttgcagt tcagggggat agatttcctg gccaagatcc acaagtctct
     1681 ggcgcacagc cggatcggcc atcgcgcgga cgatggcctg gttgagcctc gtcacgatgt
     1741 cgcctggcgt gcccttcggt gcccagaccg cattccattg caaaagataa aggccaggca
     1801 cgcccgcctc atccgcggtc ggaagatcgg gcaccgccgc caatcgcgtt ttggacatga
     1861 cggcaaagcc cttgatggca ccgctgcgaa tctgcggtat cgatgtggtg ggggtatcaa
     1921 tcgcgatgtc aaactggcca gccagcaaat cctggatcat cggcgcggcg ccgcgataag
     1981 ggacaaactg atattttgtg cccgtcacgt tttgaaggaa tacccccccg atgtggccaa
     2041 acccgccgac acccggcgtg ccctgcgtgg cagtttctgg attggccttc aaccacgcaa
     2101 cgagttcctt caggtcattt gccggcagat tcttgcgtcc gacaatgagc cccggattgt
     2161 ttgacaacaa agcgatcggc gcaaagtcgg cgagcgtatc gtattgaagc ttgtaaatcg
     2221 ctgcattggc cacatgcgtg ctccattggc cgacgatgag cgtatagcca tcaggtgcag
     2281 cacgggcgac ccggcccaca ccgaggctgc cggcagcacc ggcgatgttc tcgacaagca
     2341 agggttgacc taacgagcct cgcatgtgct cggccagaat tcgagcaatc gtatctaccg
     2401 gcccgcccgc cggaaatggc gcgaccaatg taatcggccg cgtcggatag gcttgcgccc
     2461 tcgctatccc cgaaaatgcc ggcagcacgg cggcgcccgc ggccagatgc agaagtttgc
     2521 gacgggaaag tttcattggt ttcctcccct gcgtcgtccc gccatgcgcg ctcggacgct
     2581 caattcttga acgatgaagc tgggacgtcc ccgattgcgc ccgcaccgcc gattgcaatt
     2641 accgggattc cggcacgccc tccgctgcgg ctcgggtaaa atgatacatc agctttgcga
     2701 gccggcggaa ggggcgcgcc gacataaagc cggtcatcaa tgtccgttgt gggggttcaa
     2761 tcccggcatt ccgcttgtcc gggctacgat gcgatcacaa cctgtcgtgt cccggatgtg
     2821 gtgcagcctg ccaacgctgc gctgcagagc cgggacccat tggcgcgacg tgctcgatgg
     2881 gccccggatc ggcagcgcac cgctgacgcg ctgcgcagca tcggggcacg cagcccctgg
     2941 acgttggctt tgctgctcta cggttcgcta ggccgtgcca gtcagcttgc tcaaccagct
     3001 tttcggggca gcatcggctt ccgcgggcgc ctcggtctcc ccgcccacaa cctctccaaa
     3061 cgaagcgaaa aactcacccg ccaatttctt cgccgttgaa tcgatcagcc gttggccaag
     3121 ctgcgccagc ttgccgccga tctgggcgtc gacctcataa tgcaggatcg tgacctcggg
     3181 gctttcggat tccagccgaa cggcggcgcc gcccttggca aagcccgcca caccgccgga
     3241 gccttcgccg gaaatgcgat agctgttcgg cgggtcgaga tcggacagcg tcaccttgcc
     3301 gccgaacgcg ccttgaccgg gccgacccgg aacaccacgg tggcagtcat ttcggtcggc
     3361 gacgacattt cgaggctttg gcagcccgga atgcattttt tcaggatttc gggatcgttg
     3421 agcgcggccc agactttttc cttcggcgca gggatgcgct ggctgtcatt catttgcata
     3481 actggatccc ccctaaacat ttgtggtggt gcgctgtccg caccggcgca gggtggtgat
     3541 ctcggccagg atcgacattg cgatctcttc cggcgtgatg gcgccgagat cgagaccggc
     3601 cggcgccttg atgcggtcga gcgcgctcga gtcaatgcct tcagcgatca gtttggcgcg
     3661 aagcgctgcc atcttgcggc ggctgccgac aaaggcgtga taggcggcgt cgatcccgac
     3721 cgaagccttc aaggcggcct catcgccctt gccctgcgtc gagatcacga caaagcggcg
     3781 ggcgtcgttg agatagcgcg gctgaaaacc atcgaccacg acgtgggcgt cggactcgga
     3841 ggcaacatcg gcagcgggcg ccgcgagcgt gacgtggtag ccgagctgcc gcgcctgggc
     3901 tgccagcgac agcgccaccg ggcttgcgcc gagaatcacc aatgagggat gcggcagcac
     3961 cggttcgaca aagatgtcca tggtgccctt gctcgggcac atgttctctg cgaagcgcac
     4021 gccgtcgcgg ttttcgccgg gcttgacgcc gagttcggcg agaagatttt cgggctgcac
     4081 cgagaccatg cgcggctcgc cgtcggccag tgcttcgcgc gcagcccgga gcaccgcgcc
     4141 gcgggcgcag ccgccgccga tccagcccgc cacgatggtc ccatcgggcc ggatgatcgc
     4201 cttggcgccg gccttggccg cggtcaccga tacggtgcgg accaccgtgg ccagaacaaa
     4261 ggtttcctcg gcggccttga gccgcgccac cagctccatc acttcgacat gcgcggtcat
     4321 cggcgtcttc ctcagagttt cgccagatag ggttcaagcg cggtcaggct tttcagcgtg
     4381 ttggcgggcg cgtagagatc gacatgcggc aatgcggcct tgatgccggc ggcctcgggc
     4441 gcgtagccct cccacgccat catcggattg agccagacga tgcggcggca gcgttttgcc
     4501 agcgccgcca tctcgcggcc gagcaatgcg gcgtcgccgg tctcgtagcc gtcggatacg
     4561 atcatcacgc aggcgcgcga atgcagcacc cgcgcggcgt gccagcgatt aaaggtctgc
     4621 aggctctcgc cgattttggt gccgccgcct gtgccctgcg ccatgatcga cagccggtcg
     4681 agcgcgcggc cggcatcctt ttccttcatc gcgtcggaga cataagcaag ccgcgtatgg
     4741 aacaggaaag cctcggcttc gcgaaactca tcgagcacgc cgtggatgaa gcgcaagaac
     4801 acgccggtat acatgctcat cgagccggac gcgtcgagca gcatgaccag gcgcagcggc
     4861 ttttccttgt gttgccgttt gacgagactg atcggcacgc cgccatggct gatattgttg
     4921 tggatggtcc gtcgcaaatc gagccggtag ccgcgccgcc gcgccagatc gcggcgggtc
     4981 agccgcgtcc gcatggtttt tgccagcctt gctgcgacag catgggcctc atcgatctgg
     5041 tcgggatcgg ccatgttgcg gaaatcaatc tcggcaagat tgtccgcgcg cgaggcacct
     5101 tccatgcggc cttcgccggt gcgattctgc ggcgcatcgt catcagacgg aatctggtcg
     5161 gtggcggctt cgctgccggt tcgctcggac ttgctgtcct gcaggctctt cagcgacggg
     5221 ctgttggccg ccttcgccga gcccatggtc accgatcgcg atttcacccg cttgcccaac
     5281 cagaacgcgt cgaacagccc atcgaatttt tcccaatcgg atttccgcgc cgagaacaga
     5341 tgcttgaacg cggagcgcag caaagccggc ttttcggcat aacccgccgc catcaatgcc
     5401 gcggcatcgc ggccttcatg caggccgacg gcaaaggcat tgtcgcgcaa cgtcttgaga
     5461 aacgccgcga gctttgtcgc gaccaggcgg gagacctcgt cgagctcttc gtatccggga
     5521 ttggtgccgc agcaggtcat gcgaccttcc ccagcaggcg ttcggtgact tcgcgcgtga
     5581 cgcgggattt gtcttcatgg gttttcagca ggcacatcag ggtttcgtgg accacttcgg
     5641 gcgcgtcatg cagatcgcgc acctcaagcc cgaccagcgc cgccgcccag tccagcgtct
     5701 ccgcgacccc cggcaccttg cgcagctcct ccttgcggac gttttcgacc atgcgcgcga
     5761 tctgcagcga cagcgaggcg ctcgcgccgt tgatccgcgc catgatgatg cgcgcttcgc
     5821 ggtcgacgtc gggataatcg acatagtggt agaggcagcg gcggcgcagc gcatcggaca
     5881 gttcgcgggt gccgttcgaa gtcagcacca catgcggaat ggtggtcgcc gatatcgtgc
     5941 cgagttcggg aatcgaaacc tgaaaatccg acaagagttc gagcaggaat gcctcgaact
     6001 catcgtcggc gcgatcgatt tcgtcgatca agagcaccgg cggcttgtcg cggcggatcg
     6061 ccgccagcag cggccgctcc agcaggtact tttccgaaaa gatctggtcc tcgatcgcat
     6121 cggcgtcgcc gccgcgatgc gcctggatcg cgagcagttg ccgctggtaa ttccattcat
     6181 agagcgcgga tgactggtcg agaccctcat agcattgcaa ccgaatgagc tcggtcgcat
     6241 gtgccgccgc cagcgctttt gccacttcgg tcttgccgac gcccgcctcg ccttccagca
     6301 atagcggacg ccggagcaac tgcatcagcg agatcgcggt cgcaagttca gcgtcggcga
     6361 tgtagccgct cgccgccaga cgttccgcga tctcttcgcg atctctcatc gttacgcgaa
     6421 cactcccaga ttcttcgccg cccgccagtt gcgccagtag tcgtgcggca tctggatgtg
     6481 ggtcgagcct aagaacgaga aggcgtcgtt gacggcgttg gaaaacgccg gcacgccgcc
     6541 gacattcggg ctctcgccga cacccttggc accgatcggg tgatgcggcg acggcgtcac
     6601 ggtgaagtcg gtctcccagt gcggcgtctc gaccgcggtg ggcatgaaga aatccatgaa
     6661 cgaaccggtc accacattgc cttcgtcgtc gtagcggatc tcctggccca tcgcgatcgc
     6721 gaacgcttcg gtcagtcccc catgcacctg gccctcgatg atcatcgggt tgatgcgggt
     6781 gccgcaatcg tcgagcgcgt agaagcgcct gatcttgtag accccggtat cgacgtcgat
     6841 gttcatcacg cagatatagg cgccgaacgg ataggtcatg
//
LOCUS       DATNOI010000709         3471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2238151,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000709 DATNOI010000000
VERSION     DATNOI010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2238151"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1071
                     /locus_tag="VN968_28245"
     CDS             199..1071
                     /locus_tag="VN968_28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL33160.1"
                     /translation="MKSVIVTGGSGKAGRAAIRELLKHGYQVMNVDTAAPAEQLCHFM
                     KTDLNDLGQAVDAVRLAAGTIDRRRASIGEPSAVIHLAGIPAPGLAPDATIFQNNMMT
                     TYNVFSAAIRAGIKRVVWASSETVYGLPFTRTPPIFAPVTEDHPLAPESGYALAKTLC
                     EKMASEVNRWNPGTRFVGLRISNIFEAKDYEQIPSFWADPAQRKWNLWSWVDSRDVAQ
                     ACRLGLEADVAGAANFTIAAADTLMRTPSQELMASAFPGVPVDPKLGPFDTLLSIEKA
                     RRVLGYSPQYTWRS"
     gene            complement(1119..1748)
                     /gene="mobA"
                     /locus_tag="VN968_28250"
     CDS             complement(1119..1748)
                     /gene="mobA"
                     /locus_tag="VN968_28250"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HXL33161.1"
                     /translation="MTTEFPATPGVLLAGGLARRMGGGDKPMREIGGRTILARVIARL
                     KPQCDRLILNANGDPARFAAFGLPVIADTVENFPGPLAGILAALDWAAANRPDVSWIL
                     SAAADCPFLPRDLVARLHRARAEQNAQLAVAASDGQSHPVIGLWSVALREELRHALVI
                     EDIRKIDRWTARYRLATVEWPVDELDPFFNANTMDDIAAAERLAALDGG"
     gene            complement(1738..2628)
                     /gene="fdhD"
                     /locus_tag="VN968_28255"
     CDS             complement(1738..2628)
                     /gene="fdhD"
                     /locus_tag="VN968_28255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HXL33162.1"
                     /translation="MMKIDKAPVPLIVPNPHDPRLTERVAGTDQAGAPVEINVPVERP
                     LTLYLNAQEIVTMMTIGDYPEYLALGYLLNQNMLKYDDVVTEVEYDDDLQVVVVRTEH
                     HTNFEQKLKKRTQTSGCAQGTAFGDLLEAVESVALPKAELRTSWLYQMTHAINTMPSL
                     YLEAGAIHGCVLCKEGTPVCYTEDVGRHNAVDKIAGWIYRHGVDPADKILYTTGRLTS
                     EMVIKTVRMGIPILVSRSGFTAWGVDLARQVGLTLVGRARGKRFIALSGQERIIYDQN
                     LAFVEEESARHKRKGGDRDD"
     gene            2722..3249
                     /gene="mobB"
                     /locus_tag="VN968_28260"
     CDS             2722..3249
                     /gene="mobB"
                     /locus_tag="VN968_28260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HXL33163.1"
                     /translation="MKVIGLAGWSGAGKTTLLTRVIPHLLGQGLRVSVIKHAHHSFDV
                     DVPGKDSWVHRRSGAVEVLVSSGKRWALMHELRGASEPRLPELLAKMSRVDLVIVEGF
                     KSEPHRKIEVHRLANGKAMLFPDDPGIVGIASDAAVETSLPVAHLDDIPAIAAMMQRS
                     AISVEDVLARSARQT"
     gene            3258..>3471
                     /locus_tag="VN968_28265"
     CDS             3258..>3471
                     /locus_tag="VN968_28265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HXL33164.1"
                     /translation="MAQLSDDCFAFGGPMMSVDEAVSIIAARVTAVQDIETVSLAAAD
                     GRILAKEICAPLPLPPFTNSAVDGYAV"
BASE COUNT          622 a         1079 c         1116 g          654 t
ORIGIN      
        1 agcgccatgg caatcgcagc tccgatgcca cgggacgcgc cggtcaccaa cgcggtgtgg
       61 ccgttcaaat ccggcttgat ggtcatgatt gaagtccttg ttggccgtgc agtcggctgt
      121 caatctagcg gaagcccgca ataacggcca cgatcttcgc gttgagacga agaggatagg
      181 gcatggaggt agagccagat gaaaagcgtc attgtgacag gcggaagcgg caaggcgggc
      241 agggcggcta tccgcgaatt gctcaagcac ggctatcagg tgatgaatgt cgacacggcg
      301 gcgcccgcgg agcagctatg ccatttcatg aagaccgacc tcaatgatct cggtcaggcc
      361 gtcgatgccg tgagactggc cgccggcacg atcgaccgcc gtcgggcctc catcggcgag
      421 ccgtcggctg tcattcacct ggctggcatc cccgcgcccg gtctcgcgcc ggacgcgacg
      481 atcttccaga acaacatgat gacgacctac aacgttttca gcgccgccat tcgcgccggc
      541 atcaaacgcg tcgtatgggc atcgagcgaa accgtctatg gcctgccgtt tacccggacg
      601 ccgccgattt ttgcgccggt caccgaagat catccgctcg cgccggaatc gggttatgcg
      661 ctggcgaaga cgctgtgcga gaaaatggcg agcgaggtga accgctggaa tcccggcaca
      721 cggttcgtgg gtttgcgcat ctccaatatc ttcgaagcaa aagactacga acagatcccc
      781 tcattctggg ccgacccggc ccagcgaaaa tggaacctgt ggagctgggt cgatagtcgg
      841 gatgtcgcgc aggcctgcag gctggggctc gaggcggacg tagccggcgc cgccaatttc
      901 acgattgccg cagccgatac gctgatgagg accccgagcc aggaactgat ggcatcagct
      961 tttccaggcg ttccggtcga tcccaaactt ggtccattcg atacgctgct ttcgattgaa
     1021 aaggcgcggc gggtactcgg ctactcgccg caatatacct ggcggtcgtg aattggtggt
     1081 gcgatccccc tcggttccga tgcgggccga ccgcccgttc agcccccatc caacgccgct
     1141 agacgttcgg ccgcggcgat gtcatccatg gtgttggcgt tgaagaacgg atcgagttcg
     1201 tccaccggcc actccacggt ggcgagccgg tagcgcgcgg tccagcggtc gatctttctg
     1261 atatcttcga tcaccagggc gtggcgcagc tcttcgcgca acgcgacgct ccacaatccg
     1321 atcaccgggt gcgactggcc gtcggaggcg gcgaccgcaa gctgcgcgtt ctgttcagcg
     1381 cgggcgcgat gcaggcgcgc gacgaggtcg cgcggcagaa acggacagtc cgcggcagcg
     1441 ctgaggatcc acgatacgtc gggccggttc gccgcggccc agtcgagggc cgccaaaata
     1501 cccgcaagtg gccctggaaa attctccacg gtgtcggcaa tgacgggcag gccgaatgcc
     1561 gcgaagcgtg cggggtcgcc attggcattg agaatgagcc ggtcgcattg cggcttcagc
     1621 cgcgcgatga cgcgcgcgag gatggtgcgg ccgccgatct cgcgcatcgg cttgtcgccg
     1681 ccgcccatgc gtcgcgccag cccgcccgcc agcagcacgc cgggcgtcgc cggaaattca
     1741 gtcgtcacgg tcgccgcctt tgcgcttgtg gcgcgcggat tcctcttcga cgaaagccag
     1801 attctgatcg tagatgatgc gctcctggcc cgacaatgcg atgaagcgtt ttccccgcgc
     1861 acgcccgacc agggtcagtc cgacctgccg cgccagatcg acgccccagg cggtgaaacc
     1921 cgaacgggaa accagtatcg gaatccccat ccgcaccgtc ttgatcacca tctcggaggt
     1981 cagccgtccg gtggtgtaga gaatcttgtc ggcgggatcg acgccgtggc ggtagatcca
     2041 gccagcgatc ttgtcgacgg cgttgtggcg gccgacgtct tcggtgtagc agaccggcgt
     2101 gccttccttg cacagcacgc agccgtggat cgcaccggcc tcgagataga gcgacggcat
     2161 ggtgttgatg gcgtgcgtca tctgatagag ccaggaggtg cgcagctctg ccttcggcag
     2221 cgcgacgctt tcgaccgctt ccaggagatc gccgaacgcg gtgccctgcg cgcagcccga
     2281 agtctgcgtg cgcttcttca gcttctgttc gaagttggtg tggtgctcgg tgcgcaccac
     2341 caccacctgc agatcgtcgt catattcgac ctcggtgacg acgtcgtcat atttcagcat
     2401 gttctgattg agcagatagc cgagcgcaag atattccgga tagtcgccga tcgtcatcat
     2461 ggtgacgatc tcctgcgcgt tcaggtacag cgtcagcggc cgctccaccg ggacgttgat
     2521 ctcgaccggc gcgccggcct ggtcggtccc ggcgacgcgc tcggtcagcc gcggatcgtg
     2581 cggatttggg acgatcaggg ggacgggggc tttgtcgatc ttcatcatcg ttcccaggct
     2641 agcatgacat tccctgctgg ccgatataaa gcacctttgg acaaagcata gcccggttcc
     2701 gccggaggct cagagaatat gatgaaagtg atcggcctgg cgggatggag cggcgccggc
     2761 aagacgacgc tgctgacccg cgtcatcccc catctcctcg gccagggcct gcgcgtttcg
     2821 gtcatcaagc acgcccacca tagtttcgat gtcgacgtgc cgggcaagga ttcctgggtg
     2881 catcggcggt cgggcgccgt cgaggtgctg gtgtcctccg gcaagcgttg ggccttgatg
     2941 catgaattgc gcggcgccag cgagccgagg ctgccggaat tgctggcgaa gatgtcgcgg
     3001 gtcgatcttg tcatcgtcga aggtttcaag tccgagccgc atcgcaagat cgaggtgcat
     3061 cgcctcgcca acggcaaggc gatgctgttc cctgacgatc ccggtatagt cggcattgcg
     3121 agcgatgcgg cggttgaaac ctcgctgccc gtcgcccatc tcgatgacat ccccgccatc
     3181 gccgctatga tgcagaggtc cgcgatatcc gtcgaagacg tgctggcgcg aagcgcacgc
     3241 caaacctgag atgtgaaatg gcgcaattgt ccgacgattg ctttgccttt ggcggcccga
     3301 tgatgtcggt cgatgaagcc gtcagcatca tcgccgcgcg agtgacggcg gtgcaggaca
     3361 tcgagaccgt ttcgcttgcg gcggccgatg ggcgcatcct cgcgaaggag atttgcgctc
     3421 ccttgccgtt gccgcctttc accaactccg ccgtcgatgg ctatgcggtg c
//
LOCUS       DATNOI010000710         3932 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2617280,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000710 DATNOI010000000
VERSION     DATNOI010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2617280"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="VN968_28270"
     CDS             <1..1443
                     /locus_tag="VN968_28270"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HXL33165.1"
                     /translation="LDGVVLTTGCDKTTPACLMAAATVNLPAIVLSGGPMLNGWHNGE
                     RSGSGTVVWKARERLAGGEIDYEEFMNIVASSAPSVGHCNTMGTASTMNSLAEALGMS
                     LPGCAAIPAPYRERGQIAYETGARIVGMVWEDLKPSDILTRKAFENCIVVNSAIGGST
                     NAPIHINALARHIGVELSIDDWQKFGHEIPLLVNMQPAGFYLGEEYHRAGGVPAVVRE
                     LMAQRRIHEDAMTVNGHTMGENCRNAPKPDGDVIRSYDKPLVKDAGFLVLRGNLFDSA
                     IMKTSVISKEFRDRYLVNPKDPNAFEGRAIVFEGPEDYHERIDDPSLDIDERCILFVR
                     GTGPIGYPGGAEVVNMQPPAALIKRGILSLPCIGDGRQSGTSGSPSILNATPEAAAEG
                     GLAILKTGDKVRIDLNKGSANILISEEELKRRRDELKARGGFPYPANQTPWQELYRST
                     VGQQATGACMEFATRYHDIAGRVGVARDNH"
     gene            1578..2312
                     /locus_tag="VN968_28275"
     CDS             1578..2312
                     /locus_tag="VN968_28275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXL33166.1"
                     /translation="MGDRLKGKRAFVTAGAAGIGRASAVAFAREGASVFATDIDEKGL
                     AALRNDGVSEVTRLDVRDSAAVAAMAKRVGKVDILLNAAGFVHHGTVLDCSDEDWDFS
                     FDLNVKSMHRTIRAFLPGMLESGRASIVNISSAAGVFKAAPNRYVYGATKAAVAALTR
                     AVAVDFITRGIRCNCICPGTIETPSMLQRAATAGPGGREMFVSRQPMGRLGTAEEIAS
                     LAVYLASDESAFTTGVAHIIDGGWTL"
     gene            2518..3264
                     /locus_tag="VN968_28280"
     CDS             2518..3264
                     /locus_tag="VN968_28280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL33167.1"
                     /translation="MNKIDLNGRCAVVTGGAQGFGRAITERFVASGAKVAIWDHDQPL
                     ADKTAREVGDAVTAFKVDVSDLAAVEKTRDATLKAFGKIDILVNNAGVAGVNKTVWET
                     DLEEWRKVLRINLDGPFICCKAIVPDMVKQNYGRIVNIASIAGKEGNPNAAHYSASKA
                     GLIALTKSLGKELAGYEIAVNAVTPAAAKTAIFDQLTQQHIDFMLSKIPKGRFVLVEE
                     LAAMVAWLASEDCAFSTGAVFDISGGRATY"
     gene            3390..3779
                     /locus_tag="VN968_28285"
     CDS             3390..3779
                     /locus_tag="VN968_28285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXL33168.1"
                     /translation="MIREGGCLCGAVRFKAEGEPINVRICHCRNCQKAMGSPFFARAL
                     FDQEALTVAGETARYASSEALDRVFCRICGTRLFSWRKNGTAAGVALAAFDDRNAFAP
                     TNHMWIREKMDWVRLDDGLPQYPGTAP"
     gene            3858..>3932
                     /locus_tag="VN968_28290"
     CDS             3858..>3932
                     /locus_tag="VN968_28290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXL33169.1"
                     /translation="MNISPYDISVWVLPLVFAITFHEAA"
BASE COUNT          765 a         1208 c         1255 g          704 t
ORIGIN      
        1 ctggacggcg tggtcctgac caccggctgc gacaagacca ccccggcctg cctgatggcg
       61 gcggccacgg tcaatttgcc ggcgatcgtg ctttccggcg ggccgatgct gaacgggtgg
      121 cacaatggcg agcgctcggg ctcgggcacc gtggtctgga aggcgcgcga gcggcttgcc
      181 ggcggcgaga tcgattacga ggagttcatg aacatcgtgg cttcctcggc gccctcggtc
      241 ggtcattgca ataccatggg caccgcctcc accatgaact cgctggccga ggcgctcggg
      301 atgtcgctgc caggctgcgc cgcaatcccc gccccctatc gcgagcgcgg ccagatcgcc
      361 tatgagaccg gcgcccgcat cgtcgggatg gtctgggagg acttgaagcc gtcggacatc
      421 ctgacgcgga aggccttcga aaactgtatc gtggtcaatt cggcgattgg aggctcgacc
      481 aacgcgccga tccatatcaa tgcgcttgcc cgacacatcg gcgtggagct gtcgatcgac
      541 gactggcaga agttcggcca cgagattccg ctgttggtca acatgcagcc ggcggggttc
      601 tacctcggcg aggaatatca tcgcgccggc ggcgtgccag ctgtggtgcg cgagttgatg
      661 gcgcaaaggc gcatccacga ggacgccatg accgtcaacg gccacactat gggcgagaac
      721 tgtcgcaatg cgccgaagcc cgatggcgac gtgatccgga gctatgacaa gccgctggtg
      781 aaggacgccg ggtttctggt gctgcgcggc aatttgttcg attccgcgat catgaagacc
      841 agcgtgattt caaaggagtt ccgcgaccgc tatctggtca acccgaagga tcccaacgca
      901 ttcgagggac gcgccatcgt attcgagggt ccggaagatt atcacgagcg gatcgacgat
      961 ccctcgctcg acatcgacga gcgctgcatc ctgttcgtgc gcggcaccgg tccgatcggc
     1021 tatcccggcg gcgccgaggt cgtgaacatg cagccgccgg cggcgctgat caaacgcggc
     1081 atcctgtccc tgccctgcat cggcgatggc cgccagtccg gcacatcagg ctcgccgtcg
     1141 atcctcaacg ccacgccgga agccgccgcc gaggggggat tggcgatcct gaaaacaggc
     1201 gacaaggtcc gcatcgacct caacaagggc agcgccaaca tcctgatctc agaggaagag
     1261 ctgaagcggc ggcgcgacga gcttaaagcg cgcggcggct tcccctatcc ggccaaccag
     1321 acgccgtggc aggaactcta tcgctcaact gtcggccagc aggccaccgg cgcctgtatg
     1381 gagttcgcga cccgttatca cgatattgcc ggccgcgtcg gcgtggcacg ggacaatcat
     1441 tagcaatggt cgtcgttccg ggatgagccg ccaggcgcgg gcccggaatc catactcacg
     1501 atcgtggtta tggatttccg ggttcgcgac ttcgtcgcgc cccgcaatga cgaaagaaaa
     1561 tgaaaaagga gaacgacatg ggagaccgcc tgaaaggcaa acgcgcattt gtgaccgcgg
     1621 gggcggccgg aatcggccgg gccagcgctg ttgcttttgc gcgcgaaggc gcgagcgtgt
     1681 ttgcaaccga catcgacgag aaaggcctcg cagccctcag gaacgacggc gtttcggaag
     1741 tgacgcggct tgacgtgcgc gacagtgccg ccgtggccgc aatggcaaaa cgcgtcggca
     1801 aggtggacat cctgctcaac gcggccggct ttgtgcatca cggcacggta ctggactgtt
     1861 ccgacgagga ctgggatttt tcgttcgacc tcaatgtcaa gtcgatgcac cgcacgatcc
     1921 gcgcgtttct gccgggcatg ctggaaagtg gccgggcctc gatcgtgaac atttcgtccg
     1981 ccgcaggcgt cttcaaggcg gcgcccaacc gctacgtcta cggcgcgacc aaggctgcgg
     2041 ttgcagcgct tacccgcgcg gtcgccgtcg atttcatcac caggggcatc cgttgtaatt
     2101 gtatctgccc cggcaccatc gagacgccgt cgatgctgca gcgcgccgcc accgcgggtc
     2161 ccgggggccg cgagatgttc gtgagccggc agccgatggg acggctgggc acggcggagg
     2221 aaatcgcctc gctcgccgtt tacctcgcca gcgacgagag cgcgttcacg accggcgtcg
     2281 cgcatatcat cgatggcggc tggacgctgt gattcttaac ctcgccccgc tctttgcggg
     2341 gagaggtcgg aaacttggcg aagctaagtt tccgggtgag gagctaggca ccgtgcccaa
     2401 cttgtccgcg tcgctcgcgg acggaagagc ccctcacccc aaccctctcc ccgcaaagag
     2461 cggggcgagg gagcagacca ccggcgccgc aaagatagaa gtgaaaggac tctttgcatg
     2521 aacaagatcg atctgaacgg acgctgcgct gtcgttaccg gcggagcgca gggttttgga
     2581 cgggccatca ccgagcgctt tgttgcttcc ggcgcaaagg tcgcgatctg ggatcatgac
     2641 cagccgctcg ccgacaagac cgctagagaa gtcggcgacg cagtcacggc gttcaaggtc
     2701 gatgtctccg atctcgccgc ggttgaaaag acgcgagacg caacgctcaa agcgttcgga
     2761 aagatcgaca ttctcgtcaa taatgccggc gtcgccggcg tcaacaagac ggtttgggaa
     2821 accgacctcg aggaatggcg caaggtcttg cgcatcaatc tcgacgggcc cttcatctgc
     2881 tgcaaggcga tcgtgcctga catggtcaag cagaattacg gccgcatcgt caacatcgcc
     2941 tcgatcgccg gcaaggaggg caacccgaac gccgcgcatt attcggcctc gaaggccggc
     3001 ctgatcgcat tgacaaaatc gctcggcaag gaactcgccg gctacgaaat cgcggtcaac
     3061 gccgtgaccc ccgccgcggc caaaaccgcg atcttcgacc agctgacgca gcaacacatc
     3121 gatttcatgc tgtcgaagat tccgaaagga cgttttgtcc tggtggaaga gctcgcggca
     3181 atggtggcgt ggctggcctc cgaggattgc gcgttttcca ctggcgcggt gttcgatatt
     3241 tccggcggcc gcgcgactta ttaagaaatc gaccctcatg gtgaggaggc gctcttgcgc
     3301 cgtctcgaac catgaggcca cgacgcgtta cttatccttt gagacgcggc gaagacgccg
     3361 ctcctcagga tgaggacaga gaggtcgtca tgatcaggga aggcggatgt ctgtgcggcg
     3421 cggttcggtt caaagcggag ggcgagccga tcaatgtccg tatctgccat tgccgcaatt
     3481 gccagaaggc gatggggtcg ccgttctttg cccgcgcgct gttcgaccaa gaggccctga
     3541 ccgttgcagg cgagaccgcc cgctatgctt catcggaggc gctcgaccgg gtcttttgca
     3601 ggatttgcgg cacgcggttg ttctcctggc gaaaaaacgg cacggcggca ggcgtcgcgc
     3661 tcgcagcgtt tgacgaccgc aacgcttttg cgccgaccaa tcacatgtgg atcagggaaa
     3721 aaatggattg ggtaaggctc gacgacggcc tgccgcaata tccgggcaca gctccctgag
     3781 ggaggagccc tattcgcgcc gaccgttcat gaatatgtgg tagcctgacg catcagttga
     3841 accgttcagg acatccgttg aacatctcgc cctacgacat atcggtctgg gtcttgccgc
     3901 tcgtcttcgc catcaccttc catgaggcgg cc
//
LOCUS       DATNOI010000711        14892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1360109,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000711 DATNOI010000000
VERSION     DATNOI010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 14892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14892
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360109"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VN968_28295"
     CDS             complement(<1..723)
                     /locus_tag="VN968_28295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXL33170.1"
                     /translation="MPSLVLPAPSAEAESPLPDRFQRWFAARGWSPREHQLALLEKAG
                     HDRSALLIAPTGAGKTLAGFLPTLVELSSPSPLAGEGRGGGRREGSANGLISTGRGVQ
                     RSRGLHTLYISPLKALAVDIARNLEAPIAEMNLPVKVETRTGDTPVSRRQRQRRYPPD
                     ILLTTPEQLALLLSSDDAPFLFSQLRRIVLDELHALVTSKRGDLLSLGLARLWRLAPQ
                     LRAIGLSATVAEPESLARFLVPQ"
     gene            complement(818..2038)
                     /locus_tag="VN968_28300"
     CDS             complement(818..2038)
                     /locus_tag="VN968_28300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXL33171.1"
                     /translation="MNNDQSRREFCATAIATIFASATSVSAAEKKYDPGASDTEIKLG
                     QTVPHSGPGSLYGVLGRVGEAYFQVLNEKGGINGRQVKFFTLDDAYSAPKCVEATRRL
                     VEQEEVLALFGSLGTAPQTAVHKYLNSKGVPQLLLNTGASKWNDPKNNKWTMAGLPLY
                     PTEARILAKHVVSEKPNAKVGILYQNDDFGRDFLGPFKQVLADAGGTAKVIIEQTYDL
                     TDPTVDSQLINLAKSGADVFYNISTGKAASQSIRKVAELGWKPLQLLSAGSTGRSILS
                     AAGFENATGIVAIRYAKDAGVPRWQNDPDVMAFEELRKKYLPNVDPDNTIAFAGYGQV
                     VTMAEILRRCGDELTRENVLKQANHLAGFHSPFFLDGVTYSYTPDDYTPMKTLYISIF
                     NGKDWDISDRPVTE"
     gene            complement(2145..3077)
                     /locus_tag="VN968_28305"
     CDS             complement(2145..3077)
                     /locus_tag="VN968_28305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXL33172.1"
                     /translation="MPARIFLSSGDLMADRRFDFARDLQLKGDLAAAADLLLQATELA
                     PGFASAWFTLGKIRERLGESEAAIAAFRKAQVADPDDRHGASLRLILLGAEPVSSMPP
                     AYVRALFDQYAPKFEAALVDDLGYRGPALLFKAVLSARAAIRKPALFKRAIDLGCGTG
                     LAATAFAAQVDEFTGIDLSPRMIERARDTGLYAEVEVAEMVEGLRSKPDASVDLILAA
                     DAVVYLSDLAPLLSEARRVLAPGGLLAFTAETHGDDGVILGEGLRYAHGAGYLRASVE
                     ACGLALSRCEDLSARNEDNAPVPGLVVVATKTCV"
     gene            3188..4228
                     /locus_tag="VN968_28310"
     CDS             3188..4228
                     /locus_tag="VN968_28310"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HXL33173.1"
                     /translation="MRPQDILVPVAAGLCCKPGGFYIDPVRPVERAVITHGHSDHARS
                     GHGAVLATQETLDMMRLRYGDNFAARTQAIAYGEELKLGDVTIKFHPAGHVLGSAQIA
                     VSCKDICIVASGDYKDAPDPTCAPFEVVRCDVFITEATFGLPVFRHGDAADEIRKLLA
                     SVALFPERAHLVGAYSLGKAQRVIALLRLAGYDAPIYLHGAMEKITRYYESRGVALGE
                     LRLVKGIKKADLAGTITLAPPSATSDVSTRRFPDPVTAFASGWMRVRARARQRGVELP
                     LVISDHADWNGLTATIDATGAGEIWVTHGQEDALVHWCTAKGLAARPLDLVGYGDEDE
                     SDAPPVGEDAEA"
     gene            4225..6090
                     /locus_tag="VN968_28315"
     CDS             4225..6090
                     /locus_tag="VN968_28315"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXL33174.1"
                     /translation="MNRFAELLDRLAYEPGRNNKLRLITSYFREVEDPDRGYALAALT
                     GALSFKHAKPGLIRDLIADRTDPVLFALSYDYVGDLSETVALMWPKRLDHRESSLPGY
                     PPPQPSRARGEGAAQNPEPAPRKEPEAAATLAAPLPVPSPLEGQGGGYRGPNGHDDHP
                     PHHRHNNPPPPTLTEVVTTLRTLGKTELPNQLARWLDELDETGRWALLKLVTGAMRIG
                     ISARLAKTAAAAFGDKDPHDVELMWPGLVPPYLDLFAWLEGRAEKPVNLDPTPFRPVM
                     LAHAIENTDFATLNAADFIAEWKWDGIRVQAVSGRDGRGHMVARLYSRSGEDITKSFP
                     DLLPSLHLPGAIDGELLVLRDGRVQTFNVLQQRLNRKNVSPQLVKQYPIHLRAYDLLG
                     DDSNDLRELPFVERRARLETFVKRLDDPRIDLSSTIAFDNWDTLAAARKDPAGAGAGD
                     DSEAVEGVMLKRRDAAYLPGRPRGQWWKWKRDPHIIDAVLMYAQRGHGKRSSYYSDYT
                     FGVWTSGEDGEQLVPVGKAYFGFTDEELLQIDRFVRRNTVEKFGPVRHVVHEPDQGLV
                     LEVAFEGLARSPRHKSGVAMRFPRINRLRWDKPPREADRLETLERMLKADAILPA"
     gene            6192..6494
                     /locus_tag="VN968_28320"
     CDS             6192..6494
                     /locus_tag="VN968_28320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6460 domain-containing protein"
                     /protein_id="HXL33175.1"
                     /translation="MPNDTRELPAANDGLYRFLGGSPLTVAFRLILLSILVGVVLAAI
                     GFDPWNIVNSIRRLFQRIWDLGFDAINGLWRYFLLGAVIVIPVWLLSRMFGAPRGR"
     gene            6573..7847
                     /locus_tag="VN968_28325"
     CDS             6573..7847
                     /locus_tag="VN968_28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXL33176.1"
                     /translation="MIANLTTPLIGIVSTIAIGRLGDATLLGGVAIASVIFDCLFWLF
                     GFLRMSTLAFTAQSIGAGEARELRPILVRGFVVAASIGAGLIILQIPLAAILLSLMGG
                     SEGVTRAAKIYFAIRICSAPLALANYVVLGWLVGQARARLALGVQIAINVINAAATAL
                     LVLVLDFGIAGAAIAALVAEAAGLLLGILIAGRLSRGQPAVPRATLLDRAKLMRMLAV
                     NRDIMIRTASLIAVFLFFTAQGARSGDVTLAANAVLNNFLLTSAFFLDGLANAAEQLC
                     GRAYGARDGNAFSSATRLVILWGFAFALVVAVVYALFGPRLIDIMTASEDVRRSARDY
                     LWFVVLSPMLAVFAFAFDGVYIGATWARDMRNLMMLSLLLFLGAWLAMRSFGNAGLWG
                     ALMVHYAARGGLQAFRYPALLRASFPRKPCSG"
     gene            7865..8599
                     /locus_tag="VN968_28330"
     CDS             7865..8599
                     /locus_tag="VN968_28330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXL33177.1"
                     /translation="MQLQFVGCGDALGSGGRLNTCFHVTGEHVNFLIDCGASSLPALK
                     RLAIARDAIDLILITHFHGDHFAGLPFLLLDAQFTHRTRPLVVAGPEGIETRLTQVME
                     ALFEHSSKTKQRFDLSVVALKPEETQAFGAVKVTPFPVVHGLSGGPFLAYRVETEGRV
                     IAYSADTEWTETLIPAARDADLFIAEAYYYDKVVKNHLSLKTLEAHLAEIKPKRLILT
                     HMSDDMLGRLDALDYTAASDGMIVEV"
     gene            complement(8685..9779)
                     /locus_tag="VN968_28335"
     CDS             complement(8685..9779)
                     /locus_tag="VN968_28335"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HXL33178.1"
                     /translation="MIRAFDALSLPLLRWLDPEDAHRLAIQGLRLLPPVRPRTDDPKL
                     AVRAFGLNFPNPIGMAAGFDKSAEVSDALLRLGFGFVEIGTVTPKPQVGNPRPRLFRL
                     DRDEAVINRMGFNNDGGDAVLRRLAGRAHLGGIVGVNVGANKDSADRVDDYVKLIEIF
                     APVATYFTVNVSSPNTPGLRNLQQSAALDDLLARVIDARERVRQSAGDSPVLLKIAPD
                     LSLAELDDVVHVARSRRIDGMIVGNTTLARPSTLRQQTRAKEQGGLSGRPLFRLSTRM
                     VAETYVRAEGAFPLVGVGGIDSGGAALTKIRAGASLIQLYSSLIYRGLHLVEDIKNDL
                     ASTLLRTGRDSLSEIVGADAATITAEDWPV"
     gene            complement(9776..10120)
                     /locus_tag="VN968_28340"
     CDS             complement(9776..10120)
                     /locus_tag="VN968_28340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HXL33179.1"
                     /translation="MRMIYKICSASAWREAERQGVYRGSADDVRDGFIHFSLPSQVAE
                     TARKHFFGQTGLFLIAVDADGLGDALRWEPSRNEELFPHLYGELDLGAVVAVRDLHAR
                     SDGHHDIPELAP"
     gene            10405..11058
                     /locus_tag="VN968_28345"
     CDS             10405..11058
                     /locus_tag="VN968_28345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXL33180.1"
                     /translation="MARHPKAPRTLTHDQIWTALDRLAARAGLSPSGLAKRSGLDPTT
                     FNKSKRVTPDGRERWPSTESVSKALTATSSSIDTFVQLIGDSARTVQSVPLLGFALAG
                     TGGNFDDGGFPAGKGWDEVGLPSVNDEHAYALEIAGDQMKPAYRDGDVIVVSPATPIR
                     RGDRVVVKTRDGEVMVKELKRRTAKTLELQSLNPNHVDRTLAAANVEWIARIVWVSQ"
     gene            complement(11176..11958)
                     /locus_tag="VN968_28350"
     CDS             complement(11176..11958)
                     /locus_tag="VN968_28350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXL33181.1"
                     /translation="MQFRVSPKALDDCSAEEWQARVDLAAAHRLAYMHGFSEGIFNHL
                     TLVVPGSSDRYYQIPFGMHWSEVTASSFMEVGIDDGKVKRGAGDVERSCYCIHAPIHK
                     ALPQAKAVFHTHMPYASALTRLEDPRIKEIGQTEVGMSGEIAYDDEYTGPAFDPAEGA
                     RLARVIGDKTVLFMANHGISTVGETVADAYDRLYYIERAAQVQVYAMWTGQPLKQLPA
                     AVVEKTKRDIGGAELYQGPTPAQRHFDALKRILDRKEPDYAS"
     gene            12150..12635
                     /locus_tag="VN968_28355"
     CDS             12150..12635
                     /locus_tag="VN968_28355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL33182.1"
                     /translation="MTEESKLKLPGFDPGVVAESNFTSYPELHRAANQMRYNRRLGDH
                     AGLRNFGVNLTRIVPGGQSSYRHAHSRQDEFVLVLEGEVVLQTNDGEQTLKPGMCAGF
                     PAGSGDAHRFVNRSATDAKLLVIGDRSSGDEITYPDVDMHAVLSPEGIYRYRTKSGEP
                     L"
     gene            complement(12784..14424)
                     /locus_tag="VN968_28360"
     CDS             complement(12784..14424)
                     /locus_tag="VN968_28360"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HXL33183.1"
                     /translation="MSTIDLAVSPSDLRTLAEQSNAWPFEQAKQIVARLKKKPKDEVL
                     FATGYGPSGLPHIGTFGEVARTTMVRHAFRVLTEDRIKTRLIAFSDDMDGFRKVPDNL
                     PNKEMLAEHLGRPLTRVPDPFGTHSSLGAHNNARLRAFLDTFGFDYEFASSTEYYTSG
                     KFDATLRLVLERIEKVMAVMLPSLREERAASYSPFLPICPRTGVVLYVPIVAHDVKAG
                     TISYDDPETKERVTLPVTGGHCKLQWKPDWAMRWVALGIDYEMAGKDLIDSVKLSGKI
                     CSALGGLPPEGFNYELFLDDKGQKISKSKGNGLTIDEWLRYASPESLSLFMYREPKSA
                     KRLYFDVIPRNVDDYQQFLDGFSRQDAKQQLANPVWHIHAGHPPAADMPVTFQLLLTL
                     VSSSNAENAATLWGFIGRYRPGVTPQTHPKLDAMVGYAINYYRDFVAPTKKFREPTDA
                     ERVALQDLRDALSQLPANSSAEDIQNVVYEIGRREPFLDAVKKGKDGRPGVSLDWFNM
                     LYQVLLGQEKGPRFGSFVAVYGLPNAVTMIDGALARSG"
     gene            complement(14581..>14892)
                     /locus_tag="VN968_28365"
     CDS             complement(14581..>14892)
                     /locus_tag="VN968_28365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXL33184.1"
                     /translation="DAELHSYPNDDALRLALRRGEVDFIFGDAISLAFWINGTDSAEC
                     CAFSGGPFVESRYFGEGIGIAVRKGNDLLRQSLNWALFRLWEKGRYTDLWLRYFSVSP
                     F"
BASE COUNT         2696 a         4730 c         4691 g         2775 t
ORIGIN      
        1 ctgcggcacc agaaaccgcg ccagcgattc cggctccgcg acggtggccg acagcccgat
       61 cgcgcgcagc tgcggcgcca gccgccacag ccgcgccagg ccgagcgaca acagatcgcc
      121 gcgcttggaa gtgaccagcg catgcagctc atcgagcacg atgcgccgga gctgcgagaa
      181 cagaaacggc gcgtcgtcgg acgacagcag cagcgccagt tgttcgggcg tggtgagcag
      241 aatgtccggt ggatagcgcc gctggcgctg ccggcgcgac accggggtgt cgccggtgcg
      301 ggtctcgacc ttgaccggta aattcatttc ggcgatcggc gcttcaaggt tgcgcgcgat
      361 atcgaccgcg agcgccttca gcggcgagat gtagagggtg tggaggccgc gagatcgctg
      421 cacgccgcgg ccggtcgaaa taaggccgtt ggcgctgcct tcgcggcgtc cccctccccg
      481 accctctccc gcaagggggg agggagaaga cagttccacc agcgtcggca gaaatccggc
      541 cagcgtcttg ccggcgccgg tcggcgcaat cagcagcgcc gagcgatcgt gcccggcctt
      601 ttcgagcaac gccagctggt gttcgcgcgg cgaccatccg cgcgccgcga accatctttg
      661 gaagcggtcg ggcagcgggg actcggcttc ggccgaaggc gcgggcagga caagagacgg
      721 cacgccttaa cagctaagcc gtcagagctg cttcgtcgag ggccactcgg tcacctcgcc
      781 cggcgaagag cggagcgagg gagatcggcg ccgctcgtta ctccgtgacc ggccggtcgg
      841 aaatgtccca gtccttgccg ttgaagatcg agatgtagag cgtcttcatc ggcgtatagt
      901 cgtccggtgt gtagctgtag gtcacgccat cgaggaagaa cggcgagtgg aagcccgcga
      961 ggtgattggc ctgcttcagg acgttctccc gcgtcagctc gtcgccgcag cggcgcagga
     1021 tttcggccat ggtcacgacc tggccgtagc cggcaaaggc gatggtgtta tcgggatcga
     1081 cattgggcag atatttcttg cgcagctcct cgaacgccat cacgtccgga tcattctgcc
     1141 agcgcggcac gccggcatcc ttggcgtagc ggatcgcgac aatgccggtg gcattttcga
     1201 aaccggcggc gctgaggatc gagcggccgg tcgaaccggc ggacaacaat tgcagcggct
     1261 tccagccgag ttcggccact ttgcggatcg actgcgacgc cgccttgccg gtagagatat
     1321 tgtaaaaaac atcagcgccc gacttagcga gattgatgag ctgggaatcg accgttgggt
     1381 cggtcagatc gtaggtctgc tcgatgatca ccttggcggt gccgcccgcg tccgccagca
     1441 cctgcttgaa cggacccagg aaatcgcgac cgaaatcgtc gttctgatag agaatgccga
     1501 ccttggcatt cggcttctcg ctcacgacat gcttggcaag aatccgcgct tcggtcggat
     1561 agagtggtaa ccccgccatg gtccatttgt tgttcttcgg atcattccat ttcgacgcgc
     1621 cggtgttcag cagaagctgc ggcacgccct ttgaattgag atatttatgg accgccgttt
     1681 gcggcgccgt accgagcgag ccgaacagcg ccaacacctc ttcctgctcg accagccgcc
     1741 gcgtcgcttc aacgcacttc ggagcgctgt aggcgtcgtc caaggtgaag aacttgacct
     1801 ggcggccgtt gatgccgccc ttttcgttca gcacctggaa ataagcttcg ccgacacggc
     1861 cgagcacacc gtacagcgaa cccggaccgc tgtgcggcac cgtctgcccg agcttgattt
     1921 cggtatcgct cgcaccggga tcgtattttt tctcggcggc cgagacggat gtggcggatg
     1981 cgaaaatcgt agcgatcgcg gttgcacaga actcgcgcct cgattgatca ttgttcattg
     2041 ttgtttctcc ctagatcgta cattgatcta cattccagcg cgcccggcaa gtacgaactt
     2101 gccggtttgc gacgcaatgt cgccatttgc cggcggcgca gcgtctagac gcaagtcttt
     2161 gtggcgacca ccaccagacc cggcaccggc gcgttgtcct cgttgcgggc cgacaaatcc
     2221 tcacaccgcg acagcgcaag gccgcaggct tcgaccgacg cgcgcaggta accggcgccg
     2281 tgggcgtagc gcaggccctc gccgagaatg acgccgtcgt cgccgtgcgt ttccgccgtg
     2341 aacgccagca agccgccagg tgccagcacg cgccttgctt cagacagcag cggcgcaaga
     2401 tcggacaggt agaccaccgc gtcggcggcg agaatgaggt caacgctggc gtcgggcttg
     2461 ctgcgcaagc cctccaccat ctccgccacc tcgacctccg cataaagccc ggtgtcgcgg
     2521 gcccgctcga tcatgcgcgg cgacagatcg atgccggtaa actcatcgac ttgggctgcg
     2581 aatgcggtgg cggcaagacc ggtgccgcag ccgagatcaa tcgcccgctt gaaaagtgcg
     2641 ggcttgcgga tcgcagcgcg cgccgacagc acggccttga acaacagcgc ggggccgcga
     2701 tagccgagat catcgaccaa agcggcttcg aatttcggcg cgtattgatc gaacagcgcg
     2761 cgcacatagg ccggcggcat cgacgacact ggctcggcgc cgagcagtat cagccgcagg
     2821 cttgcgccat gccggtcgtc gggatccgcg acctgcgctt ttcgaaacgc cgcgatcgcc
     2881 gcctcggact cgccgagccg ttcgcgaatt tttccaagcg tgaaccaggc cgaggcaaag
     2941 ccgggcgcga gttcggtcgc ctgcaacaat agatccgcgg cggcggccag atcgcccttc
     3001 agctgcagat cgcgcgcaaa gtcgaaccgc cggtcggcca tcagatcgcc ggaggacaga
     3061 aatattcgtg caggcatagt ggaaaccgat cggggatcaa aaaggcaggt tggcgggaag
     3121 accgggcggg acaagacttt ggcgggagga gactcgaatg aaccttacgc cggactatat
     3181 agccaagatg cgcccgcaag acatcctggt acccgttgcc gccggtctgt gctgcaagcc
     3241 cggcggcttc tacatcgatc cggtgcggcc cgtcgagcgc gccgtgatta cccacggcca
     3301 ttccgaccat gctcgatcag gccacggcgc ggtgctggcc acccaggaaa cgctcgacat
     3361 gatgcggctg cgctatggcg acaattttgc ggctcgcacg caggccatcg cctatggcga
     3421 ggaactaaag ctgggcgacg tcaccatcaa attccatccc gccggccatg tgctgggctc
     3481 ggcccagatc gcggtctcct gcaaggacat ctgcatcgtc gcgtcgggcg attacaagga
     3541 tgcgcctgac cccacctgcg cgccgttcga agtggtccgt tgcgatgtct tcattaccga
     3601 ggcaacgttt ggtctgccgg tgtttcgcca cggcgatgcg gcggacgaaa tcaggaagtt
     3661 gctcgcctcg gtggcgctgt ttccggagcg ggcgcatctg gtcggcgcct attcgctcgg
     3721 caaggcgcag cgcgtgatcg cactgcttcg tttggccggc tacgacgcgc caatctatct
     3781 gcatggcgcg atggaaaaga tcacgcgcta ctacgagagc cgcggcgtgg cgctcggcga
     3841 gctgcgcctg gtcaagggca tcaagaaggc cgatctcgcc ggcaccatca cgctggcgcc
     3901 gccctcggcc acgtcagacg tctcgacgcg gcggtttccc gatccggtca cggcgtttgc
     3961 atcgggctgg atgcgggtgc gggcacgggc ccgccagcgc ggcgtcgaat tgccgctggt
     4021 gatttcggac cacgccgact ggaacggact gaccgcgacc attgatgcca ccggtgccgg
     4081 tgaaatctgg gtcacccatg gccaggagga cgcgctggtg cattggtgta ccgccaaggg
     4141 cctggccgcg cggccgctcg atctggtcgg ctacggcgac gaagacgaaa gcgatgcgcc
     4201 gccggtgggc gaggacgccg aggcatgaac cgttttgccg aattgctcga tcgcctcgcc
     4261 tatgagcccg gccgcaacaa caagctgcga ctgatcacga gctattttcg cgaggtcgaa
     4321 gaccccgatc gcggctacgc gctcgcagcc ctcaccggcg cgctgtcgtt caaacacgca
     4381 aaacccgggc tgatccgcga cctgatcgct gatcgtaccg atccggtgct gttcgccttg
     4441 tcgtatgatt acgtcggcga cctgtcggag acggtggcgc tgatgtggcc gaagaggttg
     4501 gaccatcgcg aatcctcgtt gccgggttac ccccctcccc aaccctcccg cgcaaggggg
     4561 gagggagccg cgcagaatcc tgagccagca cctcgcaaag aaccggaagc ggcggcgacc
     4621 ttggcagcac ctcttcctgt tccctccccc cttgagggtc agggaggggg gtaccgcggg
     4681 cccaacggtc atgatgacca cccacctcac catcgccaca acaacccgcc gcctcccacc
     4741 ctcaccgaag tcgtcacgac gctccgcacc ctcggcaaga ccgagctgcc aaaccagctt
     4801 gcgcgctggc tcgatgaact cgacgagacc ggccgctggg cattgctgaa acttgtcacc
     4861 ggcgcgatgc ggatcggcat ctcggcgcgg ctggcgaaga cggcggctgc agcattcggc
     4921 gacaaggatc cgcacgatgt cgagctgatg tggcccggtc tcgtgccgcc ctatctcgat
     4981 ctgttcgcgt ggctggaagg ccgcgccgaa aaacccgtca atctcgaccc cacgccgttc
     5041 cgcccggtga tgctggcgca tgcgatcgag aatacggatt ttgcgaccct caacgccgcc
     5101 gatttcatcg ccgaatggaa atgggacggc attcgggtgc aggcggtgag cggccgcgac
     5161 gggcgcggcc acatggtggc acggctttat tcgcgcagcg gcgaggacat caccaaaagt
     5221 tttcccgacc tgctgccgtc cttacatttg cccggcgcca tcgacggtga gctattggtg
     5281 ctgcgggacg gccgggtgca aacttttaac gtgctgcaac aacgtctcaa tcgcaaaaac
     5341 gtctcgccac aactagtgaa gcaatacccg atccacctgc gcgcctatga tcttcttggc
     5401 gatgacagca atgatctgcg cgagctgcca tttgtcgagc ggcgcgcgag gctcgagacc
     5461 ttcgtcaaac gactggacga cccgcgcatc gacctgtcgt cgacaatcgc cttcgacaat
     5521 tgggacacgc tggccgcggc gcgaaaagat cccgccggcg ccggcgcggg cgacgactcc
     5581 gaagcggtcg aaggcgtgat gctgaagcgc cgcgacgccg cctatttgcc gggccgtcca
     5641 agaggccaat ggtggaaatg gaagcgcgat ccgcacatca tcgatgccgt gctgatgtat
     5701 gcgcagcgcg gccacggcaa gcgctcgtcg tattattcgg actatacctt cggggtctgg
     5761 acatcgggcg aggatggcga gcaattggtg ccggtcggca aggcctattt cggcttcacc
     5821 gacgaagaat tgttgcagat cgatcgcttc gtgcgccgca acacagtcga aaaattcggc
     5881 cccgtccgtc acgtggtgca tgagccggat caggggctgg tgctggaagt ggccttcgag
     5941 ggcctggcgc gctcgccgcg gcacaaatcc ggcgtggcga tgcggtttcc gcgcatcaac
     6001 cggctgcgct gggacaagcc gccgcgcgag gccgaccggc tggaaacgct ggagcggatg
     6061 ctgaaagcgg acgcgattct gcccgcatag cgttttccgc cggccgacgg ccattgacgc
     6121 caacaagccg gttccccatc tcctctacct caggctatag tgcggatgcg aatgggtgcg
     6181 ggagatcttc aatgcctaac gacaccaggg aattgccggc ggccaatgac ggcctctacc
     6241 gctttctcgg aggatcacca ctgacggtgg cgtttcggct gatcctgctg tcgattctgg
     6301 tcggcgtggt gctcgccgcc atcggcttcg atccctggaa tatcgtcaac agcatccggc
     6361 ggttgtttca gcgaatctgg gatctcggct ttgacgccat caacgggttg tggcgctact
     6421 tcctgctcgg cgccgtgatc gtgatcccgg tctggctatt gtcgcggatg ttcggcgcac
     6481 cgcgcgggcg atagatcgca cgcaggccgg tttcgttgac ttccgtatcc aaggttgcga
     6541 ccgcgcaggt ctttgccatc gccggaccgg cgatgattgc aaatctgacc acgccgctga
     6601 tcggcatcgt ctcgacgatc gcgatcgggc ggctgggcga cgcgacgctg ctcggcgggg
     6661 tggccatcgc gtcggtcatt ttcgactgcc tgttctggct gttcggcttc ctgcgcatga
     6721 gcacgctcgc cttcacggct caatcaatcg gcgccggcga ggcccgtgaa ttgcgcccga
     6781 tcctggtgcg cggatttgtc gtggcagcat cgatcggcgc gggcctgatc atcctgcaaa
     6841 tcccgctcgc cgccatcctg ctgagcctga tgggcggcag cgagggcgtg acccgcgccg
     6901 cgaaaatcta cttcgccatc cggatctgct cggcgccgct ggcactggcc aattatgtcg
     6961 tgctcggctg gctggtcgga caggcgcgcg cgcgacttgc gctcggcgtg cagatcgcga
     7021 tcaacgtcat caacgcggcg gcgacggcgt tgctggtgct ggtgctcgac ttcggaatcg
     7081 caggtgctgc gattgcggct ctggtggccg aagcggccgg gctgttgctc ggcatcctga
     7141 tcgcggggcg cctttcgcgg ggacaacccg ccgtgccgcg cgcgacgctt ctcgaccgcg
     7201 ccaagctgat gcgcatgctg gcggtcaacc gcgatatcat gatccggacc gcttccctga
     7261 tcgcggtgtt cctgttcttc accgcgcaag gcgcacgctc gggcgatgtg acgcttgccg
     7321 ccaacgcggt tttgaacaat ttcctgctga ccagcgcgtt cttcctcgat ggcctcgcca
     7381 acgccgccga gcagctctgc ggccgcgcct atggcgcccg cgacggaaac gccttctcca
     7441 gcgcgacccg gctggtgatc ctgtggggat ttgcctttgc cctcgtggtg gccgttgtgt
     7501 acgcgctgtt cggccccaga ctgatcgata tcatgaccgc aagcgaggat gtccgccgca
     7561 gcgcgcgcga ctatctatgg tttgtggtgc tgtctccaat gctcgccgtg ttcgccttcg
     7621 ccttcgacgg cgtctatatc ggcgcaacct gggcgcgcga catgcgcaac ctgatgatgc
     7681 tgtcgctgtt gctgtttctc ggcgcctggc tggcgatgcg ctcgttcggc aatgcgggac
     7741 tatggggcgc gctgatggtg cattatgccg cgcgcggcgg cttgcaggcg tttcgttatc
     7801 cggcgctgct gcgggcctca ttcccgcgca agccgtgctc cggctgaatt gggaggatcg
     7861 tccgatgcaa ttgcaatttg tcggctgcgg cgatgcgctc ggttccggtg gccggctcaa
     7921 cacctgcttc catgtcaccg gcgagcacgt caatttcctg atcgattgcg gcgcatcctc
     7981 gctgccggcg ctgaagcggc tcgccatcgc gcgcgatgcg atcgacctca tcttgatcac
     8041 gcatttccac ggcgatcatt tcgcaggctt gccgtttctg ctgctcgatg cgcaattcac
     8101 ccatcggacg cgtccgctcg ttgttgctgg ccccgaagga atcgagacgc ggctgactca
     8161 ggtgatggaa gccttgttcg agcattcatc gaaaacaaag cagcggtttg acctgtcggt
     8221 ggttgcgctc aagcctgagg aaacgcaggc attcggcgcg gtgaaagtga cgccgtttcc
     8281 ggtggtgcac ggcttgtccg gcggtccgtt tctggcctac cgggtcgaga ccgaaggccg
     8341 cgtcatcgcc tacagcgcgg acaccgaatg gacggaaacg ctgatcccgg cggcgcgcga
     8401 cgccgacctg ttcatcgccg aggcctatta ttacgacaag gtcgtcaaga atcacctcag
     8461 cctgaaaacg ctggaagcac atctggccga gatcaaaccg aagcggctca tattaaccca
     8521 tatgagcgac gacatgctgg ggcggctcga tgcgctggac tacaccgccg cgagcgacgg
     8581 catgatcgtc gaggtgtaga gttttttctt gcgcgtattc tgatcgcaaa accggtatcc
     8641 accctcggat cgagtccgag gggatgcttt ttgcggaata cgcgctaaac cggccaatcc
     8701 tcggcggtga tcgtcgccgc atcagcgccg acgatttcgg acagcgagtc gcgcccggtg
     8761 cgcaatagcg tcgaggcaag atcgttcttg atgtcctcga ccaggtgaag gcccctgtag
     8821 atcagcgagg aatagagttg gatcaggctg gcgccggcgc ggatcttggt cagcgcggcg
     8881 ccgccgctgt cgatgccgcc gacgcctaca agcggaaacg cgccctcggc gcgcacatag
     8941 gtttcggcca ccatccgtgt cgacagccgg aacagcggcc tccccgacaa tcccccctgc
     9001 tctttggcgc gggtctgctg gcgcaaggtc gagggccgtg ccagcgtggt gttgccgacg
     9061 atcatgccgt cgatccggcg cgagcgcgca acgtgaacca catcgtcgag ttcggccaga
     9121 ctcagatccg gcgcgatctt gagcagcacc ggcgaatcgc cggcgctctg ccgcacccgt
     9181 tcgcgcgcat cgatcaccct tgccaggaga tcgtcgagtg ctgccgactg ctgcagattg
     9241 cgcagccccg gggtattcgg cgacgacaca ttgacggtga aataggtcgc gaccggcgcg
     9301 aagatctcga tcagtttgac ataatcgtcg acgcggtcgg cgctatcctt gttggccccg
     9361 acgttgacgc cgacaatgcc gccgagatgc gcgcggcccg ccagccgccg cagcaccgca
     9421 tcgccgccgt cattgttgaa gcccatgcgg ttgatcaccg cctcgtcgcg gtccagccgg
     9481 aacagccgcg gccgcgggtt gccgacttgc ggcttcggcg tcaccgtccc gatttcgacg
     9541 aagccgaagc ccagccgcaa cagcgcgtcc gaaacctccg cgctcttgtc gaagcccgcg
     9601 gccatgccga tcggattggg aaagttcagg ccaaaggccc gcaccgcgag ttttggatcg
     9661 tcggtgcgcg gccgtaccgg tggcagcaac cgcaagccct ggatcgccag acggtgcgcg
     9721 tcttccgggt cgagccagcg caacaacggc aacgacaggg catcgaaggc gcggatcacg
     9781 gcgcaagctc cgggatgtcg tgatgtccat cggatcgcgc gtgcagatcg cgcacggcga
     9841 cgaccgcgcc gagatcaagt tcgccgtaga gatgtggaaa cagctcttca ttgcgcgacg
     9901 gctcccaccg cagcgcgtcg cccagcccgt cggcgtcgac cgcgatcagg aacagcccgg
     9961 tctggccgaa gaaatgtttc cgggccgtct ccgccacctg cgagggaaga gaaaaatgaa
    10021 tgaagccatc gcgaacatcg tcggcgctgc cgcggtacac gccttgccgc tcggcctcgc
    10081 gccaggccga agcggaacag attttgtaga tcatgcgcac gtgcccggag tcctgcctgg
    10141 tcgttttgag tccccctcgg gattccctta aggttttcct tggattttcc ttgagatttt
    10201 ccttgagatt ttcctcggaa tttccccgga atttccggac ctggcttcga ccgtatcgac
    10261 ggcaccggcg cacttcaagg gctgccttca ggcgccagtg agtcgcttgc cagccgcccg
    10321 gcagtctctt ggcagtcgct tgcgccgcat tgcgggcatc ggttgattga ggtaataatt
    10381 cctagctttt gacaaagttg acctatggcc agacatccaa aagcgccgcg cacgctgacc
    10441 cacgaccaga tctggaccgc gctggatcgg ctggcggcgc gcgccggact gtcgccttcc
    10501 gggttagcca aacgttccgg cctcgatccc accacattca acaaatccaa acgcgtcacg
    10561 cccgacgggc gggagcggtg gccgtcaacc gaatcggtat ccaaggcgct caccgccacc
    10621 agcagttcca tcgacacttt cgtgcagttg atcggcgaca gcgcacgaac cgttcagtcg
    10681 gtgccgctgc tcggcttcgc gctggcggga accggcggca atttcgacga tggcgggttc
    10741 cccgccggca agggctggga tgaggtcggc ctgccctccg tcaacgacga gcacgcttat
    10801 gccctggaaa tcgccggcga tcagatgaag cccgcctatc gcgacggtga cgtcatcgtg
    10861 gtgtcgcctg cgacgccgat ccgccgcggc gaccgcgtgg tggtgaaaac ccgggatggc
    10921 gaggtgatgg tcaaggaact aaaacggcgg accgcgaaga cactggaact gcaatcgctc
    10981 aatccgaacc atgtcgaccg caccctggcg gccgccaacg tcgagtggat cgcacggatc
    11041 gtgtgggtga gccagtgaac cgccgtcatt gccgggctcg acccggcaat ccatcaatca
    11101 aagagacgtc ttccgaagat gatggatgcg cgggtcaagc ccgcgcatga cgcagcaaag
    11161 taaactagcc gccgcctacg acgcgtagtc gggctccttg cggtcgagga tgcgcttgag
    11221 tgcatcgaag tgccgttgtg ccggggttgg accctggtag agctcggccc cgccgatatc
    11281 ccgcttggtc ttctcgacca cggcggcggg gagctgcttg agcggctgcc cggtccacat
    11341 cgcgtagacc tgaacctgtg ccgcgcgctc gatgtagtaa agccgatcat aggcatcggc
    11401 gacagtctcg ccgaccgtgc tgatgccgtg gttggccatg aacaacaccg tcttgtcgcc
    11461 gatcaccctg gcgagacgag cgccctcagc cggatcgaac gccggcccgg tatactcgtc
    11521 gtcatacgcg atttctcctg acatgccgac ctcggtctgg ccgatctcct tgatgcgcgg
    11581 gtcttccaga cgcgtcagcg cgctcgcata gggcatgtgg gtgtgaaaca cggctttggc
    11641 ctgcggcagc gccttgtgga tcggcgcatg aatgcagtag caggaacgtt ccacgtcgcc
    11701 ggcgccacgc ttgaccttgc catcgtcgat gccgacctcc atgaaggaag aggccgttac
    11761 ttcggaccaa tgcatcccga acgggatctg gtagtagcgg tcgctgctgc cgggaacgac
    11821 aagggtcagg tggttgaaga ttccctcgct gaaaccgtgc atgtaggcca gccggtgcgc
    11881 cgccgcgaga tcgacccgcg cctgccattc ctcggcgctg caatcgtcaa gcgcttttgg
    11941 cgaaaccctg aactgcatgg acatcctccc tattggtatg gcgatggcat ccgcgccacc
    12001 gcttcaacgg agaccccgga cggttcttgt tatgctcaat attgtaacct attttggagc
    12061 ggaattggtt gaaattggcg cgggtctctc atctccattt gcggcggctt caccatccag
    12121 ttgcggacag gtcatgaaag ggagccggaa tgaccgagga atccaaactg aagcttccgg
    12181 gttttgatcc gggcgttgta gctgagagca atttcaccag ttatccggag cttcaccggg
    12241 cagccaatca gatgcgatac aataggcggc tcggtgatca cgccggcctg cggaatttcg
    12301 gcgtcaactt gacccggatt gttccgggag ggcagtcctc ttaccggcat gcgcactctc
    12361 gccaggacga gttcgtcctt gtactggagg gcgaggtggt attgcagacg aatgacggag
    12421 agcaaacatt gaagccgggg atgtgcgccg ggtttccagc cggttcaggc gacgcccacc
    12481 ggtttgtcaa tcgctccgca acggacgcaa agctgctcgt cattggtgac cggagttcag
    12541 gtgacgagat cacctacccc gacgtggaca tgcatgcagt tctcagccct gaaggcatct
    12601 atcgataccg taccaaatcc ggcgagcctc tttgagcggg gtactccact cgacgtcgtc
    12661 ccggcgtgag ccgggaccca tcataccgcg aggtctttcg ccttcgcgat atggtcgacg
    12721 ctgttcgcag caaccgaggc tgatggctgg gtccctgctt tcgcagggac gacgccacag
    12781 tacctacccc gaccgcgcca gtgcgccgtc gatcatggtg accgcgttcg gcaaaccata
    12841 taccgcgacg aacgagccga agcgcgggcc tttttcctgg ccgagcagca cctggtagag
    12901 catgttgaac cagtcgagcg agacgccggg ccggccgtcc ttgcccttct tcacggcgtc
    12961 gagaaacggc tcgcgccggc cgatctcgta gaccacgttc tggatgtcct cggccgacga
    13021 gtttgccggc aattgcgaca gcgcatcgcg aagatcctgc agcgcgaccc gttcggcatc
    13081 ggtcggctcg cggaatttct tggtcggggc cacgaagtcg cgatagtagt tgatggcgta
    13141 gccgaccatc gcgtcgagct tcgggtgggt ctgcggcgtg acgccgggac gatagcggcc
    13201 gatgaaaccc cacagggtcg cggcattctc cgcgttcgac gatgacacca gcgtcagcag
    13261 caactggaac gtaaccggca tgtcggccgc gggcggatgg ccggcgtgaa tatgccagac
    13321 cggattggcc agctgctgct ttgcatcctg ccgcgaaaag ccgtcgagga actgctgata
    13381 gtcgtcgaca ttgcgcggga tcacgtcgaa gtacagccgc ttggccgact tcggctcgcg
    13441 gtacatgaac agcgacagcg attccggcga cgcatagcgc agccattcat cgatggtgag
    13501 cccgttgccc ttcgatttgg atattttctg gcccttgtcg tcgaggaaga gttcgtagtt
    13561 gaaaccttcc ggcggcaggc cgccgagtgc cgagcagatc ttgccggaca gcttgaccga
    13621 atcgatcagg tccttgccgg ccatctcata atcgatgccg agcgcgaccc agcgcatcgc
    13681 ccagtccggc ttccactgca gcttgcaatg gccgccggtg accggcagcg tgacgcgctc
    13741 tttggtctcg ggatcatcat aggagatcgt gccggccttt acatcatgcg ccacgatcgg
    13801 cacatacagc accacgccgg tgcggggaca gatcggcagg aacggcgaat agctcgccgc
    13861 acgttcttcg cgcaaggacg gcagcatgac cgccatcact ttttcgatgc gctcgagcac
    13921 aagccgcagc gtcgcgtcga attttcccga cgtgtaatat tcggttgaac ttgcaaactc
    13981 gtaatcgaat ccgaacgtgt cgagaaacgc acgcagccgt gcattgttgt gggcgcccag
    14041 gctcgaatgg gtgccgaacg gatcgggcac cctggtcagc ggccgaccga gatgttctgc
    14101 cagcatctcc ttgttcggca ggttatccgg caccttgcgg aacccgtcca tgtcgtcgga
    14161 aaacgcgatc agccgcgtct tgatcctgtc ctcggtgagt acacgaaacg catgccgcac
    14221 catggtggtg cgcgcgacct cgccgaaggt gccgatatgc ggcaggcccg acggcccgta
    14281 gccggttgcg aacagcacct cgtccttcgg ttttttcttc agccgcgcga caatctgctt
    14341 ggcctgctcg aacggccagg cgttggattg ttcggcgagc gtacgcaggt cgctcggact
    14401 gacggcaaga tcgatcgtag acatcaaaag ggcctccggg ccgcggaaac tagcgtgttc
    14461 gcgtacaaat gcaaaacggt ggtggcgccg tcattccggg atggtgcgtt agcaccagac
    14521 ccggaatctc gagattccgg gttcgatgct acgcatcgcc ccggaatgac aggagccttc
    14581 ctaaaacggg ctcaccgaaa aatagcgcag ccacagatcg gtgtagcggc ctttctccca
    14641 gagccggaac agcgcccagt tcagcgattg ccgcaacagg tcattgccct tgcgcacggc
    14701 gatgccgatg ccctcgccga aatagcggct ctcgacaaac ggcccgccgg agaacgcgca
    14761 gcattcggcg gaatcggtgc cgttgatcca gaacgccagc gagatcgcgt cgccgaaaat
    14821 gaaatccacc tcgccccgcc gcaacgccag ccgcagcgcg tcgtcattgg gataggaatg
    14881 cagttcggcg tc
//
LOCUS       DATNOI010000712         3494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_590969,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000712 DATNOI010000000
VERSION     DATNOI010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590969"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VN968_28370"
     CDS             <1..304
                     /locus_tag="VN968_28370"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HXL33185.1"
                     /translation="SPGAVHPLVCVHPDTGRRMLYLGRRRNAYLMGLELADSEALLDE
                     LWNFVARPEFAWEHVWKVGDLVLWDNRCTMHRRDPFDDSARRIMHRTQIKGSAAPV"
     gene            304..1537
                     /locus_tag="VN968_28375"
                     /pseudo
     CDS             304..1537
                     /locus_tag="VN968_28375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1559..2017)
                     /locus_tag="VN968_28380"
     CDS             complement(1559..2017)
                     /locus_tag="VN968_28380"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXL33186.1"
                     /translation="MESIKTTYKSFHYKAKTTWSSARRGMLSAAGKPNIVVGSPLEFK
                     GEPDVWAPEELLVGSLNTCMMLTFLTLAQRQGLTPVGYESEAEGLLDNVEGKYHITEV
                     TVRPRVTLKSETELELARKSMESVDAHCFIANSINARITLTPEFVVAAAT"
     gene            2229..2849
                     /locus_tag="VN968_28385"
     CDS             2229..2849
                     /locus_tag="VN968_28385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33187.1"
                     /translation="MKRTWIVIMAVAAMAAASATVGHAQEDGIDKAKAEAFDVRMFVT
                     APGKKAYACFVRRYDPDHLARHPKQKVSAMKLLVTAEIPSDENTTNYSFRLGVKYRHR
                     SGDFDSSGFCSHVVAEDAGNETRFGCGVDCDGGGIGVALSKDDKSAIIRLERVRIWQN
                     NKPDEEAGDSLVAGADDKIFRLDRIDTRECASLVTDRKELAAIRHK"
     gene            2905..3369
                     /locus_tag="VN968_28390"
     CDS             2905..3369
                     /locus_tag="VN968_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HXL33188.1"
                     /translation="MHRRRILWGAFSALGAAFASATIRANAATDAGPDKVQGKLKVVY
                     HLNDLDKVNFVIGNIENHFDGVGGPDNVTIALVVHGQALRAFHASSANPDLSRRVGQF
                     SKAGLELAACGNTMKSQNVALKDLLPGFISADRGGVVRIAELQSQGYLYLRP"
BASE COUNT          628 a         1073 c         1074 g          719 t
ORIGIN      
        1 gtcgcccggc gccgtacacc cgctggtctg tgtccacccc gacaccgggc ggcgcatgct
       61 ctatctcggc cgccggcgca atgcgtatct catggggctc gaacttgcgg actccgaggc
      121 gctgctcgac gagctctgga atttcgtcgc gcgcccggaa ttcgcctggg agcacgtctg
      181 gaaggtcggc gacctcgtgc tgtgggataa tcgctgcacc atgcatcggc gcgacccgtt
      241 cgacgactcc gcacgccgca tcatgcaccg gacgcagatc aagggcagtg cggcgccggt
      301 ctgatgcgcc gattaggcgt ttccaatacg gtcctgctga tcatctgtct catgtacctg
      361 atcctttatg tggatcgggt caacatctcg acagccgcgc cgctgatcaa ggccgacctt
      421 gatttaggca acacccaacg cctatccgta tgcgctgttc cagctgatcg gaggctattt
      481 cggcgacaaa ttcggtgcgc gtcggacgct gttcgtcagc ggtcttatcg tatgcgcggc
      541 gacggcggca accggcgccg tcggtggaat tgcgagcctg ttcgccgcgc ggcttgcgct
      601 gggtatcggc gaaggcgcga cctttccgac tgcgacgcgc gccatggcgg cgtgggtacc
      661 ggagcgtagt tggggtttcg cccaaggcat cacgcattcg tcggcccgga tcggaaacgc
      721 gctcacgccg cctctgattg ccttgctggt cgggctggtg tcgtggcgcg gtgcgttcgt
      781 tgtccttgcg ctcttgagcc tggtctgggt gttcgtgtgg ttatggtact ttcgtgatgt
      841 accggcagcg ccgccactga ccgccgagga actcgccgag ctccccgtac gcagccgggg
      901 cgcgaccagg ccggtgccat ggttcaagtt atttcgccgc atgctgccgg tgaccgcggt
      961 cgatttctgc tacggctgga cgctgtggct gtttctaagc tggataccgt cgttctttta
     1021 ccagaactat cagcagagcc tgacgcagtc ggcattctat tcggccggcg tcttccttgc
     1081 cggcgtgatc ggcgacacct tgggcggcgt ggcgagcgac cggatcctgc accgcaccgg
     1141 cgaccgggcc gcggcacggc gtaacgtcat cgtggccggt atgctcggcg gctttctgtt
     1201 cctgattccg gtgatcctgg ttcacgacgt caacgtcgtg gcgatctcac tcgccgcggc
     1261 attcttttcc gtcgaactgg tggtcgcccc gatctgggca gtaccgatgg acatcgcgcc
     1321 aaaatattcg ggatcggcga gcggcatgat gaatttcggc ttcggcgttg ccggcatcat
     1381 ttcacccttc gtgttcggct atctgatcga caagaccgga agctggacct tgccatttgc
     1441 cggctcgatt ggcctgttgt tgctcggcgc cgggcttgcg tttcgcatgc atccggagcg
     1501 gccattcgag gtggcgcctg aaggtggccc ctcctgatca ccgttcgatt agggtcattc
     1561 acgtcgcagc agcaacgaca aactccggcg tgagggtgat cctggcgtta atggaatttg
     1621 cgatgaagca gtgggcgtcg acgctttcca tgcttttgcg ggcaagctcg agttcggtct
     1681 cgcttttcaa ggtgacgcgg ggtcggaccg tgacttcagt gatgtggtac ttgccctcga
     1741 cgttgtcgag caagccttcg gcttcgcttt catatccgac cggcgtcaga ccctggcgtt
     1801 gagcgagcgt gaggaatgtc agcatcatgc acgtgttgag cgagccgacc agcagctctt
     1861 cgggtgccca gacgtccggc tcacctttga actccagcgg gctgccgacg acgatattcg
     1921 gctttcccgc tgctgaaagc atgccacgcc gcgccgaact ccaggtggtt ttggccttat
     1981 agtggaaaga cttataggtt gtctttatgg actccacgaa aacctccgag gcctccggtc
     2041 gcagagggcg ctcgcgccac cctttactcc ttcggccatt tcccgcgcag cggccctctc
     2101 atcgtagcgg gtcacacaag cagaggcgag ccccgttgga acgtccccgc ggctgccgca
     2161 tcgtgctaca cccgattggc tagccgagcg gacgatcgat ggatgtgtca taaatggccg
     2221 agaacctcat gaaacgtacc tggattgtga taatggcggt cgccgcaatg gcggctgcct
     2281 cagccaccgt cggccacgcc caggaggacg gcatcgacaa ggcaaaggcc gaggcattcg
     2341 acgtcaggat gttcgtcacg gcgcccggca agaaggccta tgcctgcttc gtgcgccgct
     2401 acgaccccga tcatctggcg cggcatccca agcaaaaagt cagcgcgatg aagctgctgg
     2461 tgacggcgga aatcccgtcg gatgaaaaca ccaccaatta ttcgttccgc ctcggcgtca
     2521 agtatcgcca tcggtcgggc gatttcgact ccagcggctt ctgcagccac gtcgtcgccg
     2581 aagacgccgg caatgaaacg cggttcggct gcggcgtcga ttgcgatggc ggcggcatcg
     2641 gcgtagcctt gtcgaaagac gacaagtccg ccatcatcag gctcgagcgg gttcgcatct
     2701 ggcaaaacaa caagcccgac gaggaagccg gcgattcact ggtcgccggc gccgatgaca
     2761 agatcttccg gctggatcgc atcgacacgc gtgaatgcgc gtcgctggtg accgaccgca
     2821 aggaactcgc ggcgataaga cacaaatgac aggttcggcg caagttgccg cctcgcagaa
     2881 cgcaggccaa tatgggagga cgccatgcat cgacgccgca ttttatgggg cgcgttttca
     2941 gcgcttggtg ccgccttcgc gtcggcaacc atccgagcaa acgccgccac cgacgctggg
     3001 ccggataagg tgcaaggtaa actaaaggtc gtctatcacc taaacgacct cgacaaggtc
     3061 aatttcgtga tcggcaacat tgagaaccat ttcgacggcg tcggcggtcc tgacaacgtc
     3121 accatcgcac ttgtggtgca cggccaggcg ctgcgggcat ttcacgcaag ctcggccaac
     3181 ccggatctca gccgacgcgt cggtcaattt tccaaggcgg gccttgaact cgccgcatgc
     3241 ggcaacacca tgaagtcaca gaatgttgcg ctgaaggact tgctgcccgg attcatcagc
     3301 gccgacagag gcggcgtggt ccggatcgca gaactgcaat cgcagggata tctgtatctg
     3361 cgaccctgag ccccagcctt ctgaagtcac agcgccgagc cgaaaaacag ccgattgtcc
     3421 ggaatcgata tcgacccagg gcatcgatat gcgttgtcgg aaggtggcga tattcattca
     3481 accacctcgt cggc
//
LOCUS       DATNOI010000713         3604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1361471,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000713 DATNOI010000000
VERSION     DATNOI010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361471"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VN968_28395"
     CDS             complement(<1..645)
                     /locus_tag="VN968_28395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXL33189.1"
                     /translation="MSPAARRILLSVVAIVVVALGVGLWIVRGPGPLAFADGPKVALA
                     DYRGGNPTGVPAALTQTSLVERGAYLARAADCMACHTTEDGKEYAGGLGFKLPFGTLY
                     STNITSDKETGIGNYTDQDFLNAMHRGIRRDGARLYPAMPFTSYTYMTDADALAIKAY
                     LFSLPPVRAAVPENTLTFPFNQRWSMMFWSALFNPDRRFEPDASKSPEWNRGAYL"
     gene            complement(642..2825)
                     /locus_tag="VN968_28400"
     CDS             complement(642..2825)
                     /locus_tag="VN968_28400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXL33190.1"
                     /translation="MKVAEKIPDGLSRRVLLTGSLAGGFLLAFHLPLRALTPNEPVQP
                     PDDTNGKFAPNAFIRIDNAGNITLVMPQVEMGQGIYTAVAMILAEELDADFARVTLEH
                     APANEKLYANPAFGIQATGGSTSVRAFWTPLREAGASARAMLVQAAAQEWQVEPASCT
                     TANGEVVHKDSGRKLSYGALATSASSERPPKDVALKDAKDFSLIGKPLKRLDTPDKVN
                     GKTVYGIDAMLPDMKFATLKACPVFGGKVGKVDDSAARKIPGVRKIVVLDDMVAVVGD
                     HMWAAKKGLDALSVDWDEGPNARISSNDIWRDLHAASEKDGVVAKSQGDIAKGLATGD
                     RLEASYELPFLAHATMEPLNATVHVKPDSCEIWTGTQIMARVQSEAAKAAGLPVEKVT
                     VHNHLIGGGFGRRLEPDMVVAAVRVAKQVDGPVKVVWTREEDIQHDIYRPAYRDTIAA
                     TLSDGKIVGWKYKVSGASIIARWLPPAFEKGIDIDAIDSAVDMPYDIPNFHVEFVRAE
                     PPAVPTGFWRGVGANNNVFAIECFMDELARQAGKDPVDFRRGMLGKNPRPLAALNLVA
                     EKSGWGQPLPARVGRGVCVQPSFGSFIATVVEAEVDQQGEVHLRRITSAVDTGVAVNP
                     DTIVAQLEGGLIFGLTAALYGEITIDKGRVQQSNFHDYRMLRIDQAPKIEVHVIKSSE
                     APGGIGETGVTAGPPALRNAIYAATGVALRRLPIDRALIAAEKKA"
     gene            complement(2835..3299)
                     /locus_tag="VN968_28405"
     CDS             complement(2835..3299)
                     /locus_tag="VN968_28405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXL33191.1"
                     /translation="MATTLTINGEQKSFDAPADMPLLWVLRDVLGMTGTKFGCGMALC
                     GACTVHVDGKPVRSCMLPVGAVRDRAITTIEGVGATPVGAKVQKAWLDLEVVQCGYCQ
                     PGQIMSAAALLAATPNPDDSDIDAAMAGNICRCGTYVRIRAAIKQAASQRQS"
BASE COUNT          619 a         1152 c         1151 g          682 t
ORIGIN      
        1 gagataggcg cccctgttcc attccgggct cttcgaggcg tcgggctcga aacgcctgtc
       61 cggattgaac agcgccgacc agaacatcat gctccagcgc tgattgaacg gaaacgtcag
      121 cgtgttttcc gggacagccg cgcgcaccgg cggcaggctg aacagatagg ccttgatcgc
      181 cagcgcatcg gcgtcggtca tgtaggtgta ggacgtaaac ggcatcgccg gataaagccg
      241 cgcgccgtcg cggcggatgc cgcgatgcat cgcattgagg aaatcctgat cggtgtaatt
      301 gccgatgccg gtctccttgt ctgatgtgat gttggtcgag tacagcgtgc cgaacggcag
      361 cttgaacccg agcccgccgg catattcctt gccgtcctcg gtggtgtggc aggccatgca
      421 atccgcggct cgcgcgagat aagcgccgcg ctccacgaga ctcgtttgcg tgagcgccgc
      481 cggcaccccg gtgggattgc cgccgcgata atcggcgagc gccaccttgg ggccatccgc
      541 gaaggcaagc ggaccgggac cgcggacaat ccaaagtccg acgccgagcg cgaccaccac
      601 gatcgcgaca acgctgagga gaatgcgacg cgcggcgggg ctcatgcttt tttctccgct
      661 gcgatcagcg cacggtcgat cggcagccgc cgcaatgcga cgccggtcgc ggcgtaaatg
      721 gcgttgcgca gcgcgggcgg ccccgccgtc acgccggtct cgccgatgcc gcccggcgcc
      781 tcgctgctct tgatgacatg aacctcgatc ttcggcgcct ggtcgatgcg cagcatccgg
      841 tagtcatgga agttggattg ctggacgcgt cccttgtcga tggtgatctc gccatagagt
      901 gcagccgtca ggccgaagat cagtccgccc tcgagctgcg ccacgatggt atcgggattg
      961 acggcgacgc cggtatcgac cgcggaggtg atgcggcgca aatgcacctc gccttgttgg
     1021 tcgacttccg cttccaccac ggtggcgatg aagctcccga acgacggctg aacgcacaca
     1081 ccgcgaccga cgcgcgccgg gagcggttgc ccccaaccgg atttttctgc caccagattg
     1141 agcgcggcca gcgggcgtgg attttttccg agcatgccgc ggcggaaatc gaccggatcc
     1201 ttgcccgcct ggcgggcgag ctcgtccatg aagcattcga tggcgaagac gttgttgttg
     1261 gcgccgacgc cgcgccagaa tccggtcggc accgccggcg gttcggcccg tacaaattcg
     1321 acatggaaat tgggaatgtc gtagggcatg tcgaccgcgc tatcgatggc gtcgatgtcg
     1381 atgcccttct cgaaggccgg cggcagccac cgcgcgatga tggacgcgcc gctgaccttg
     1441 tatttccagc cgacgatctt gccgtcggac agcgtcgcgg caatggtgtc gcgataggct
     1501 ggacgataga tgtcgtgttg aatgtcctct tcgcgggtcc acaccacttt caccggcccg
     1561 tcgacctgct tcgccacgcg gacggccgcg accaccatgt cgggttcgag cctgcggccg
     1621 aagccgccgc cgatcaggtg gttgtggacg gtgactttct cgaccgggag gccggccgct
     1681 ttggccgcct ccgactgcac ccgggccatg atctgcgtgc cggtccagat ttcgcaggaa
     1741 tcaggtttga cgtgaaccgt ggcgttcagc ggctccatgg ttgcgtgcgc caggaacgga
     1801 agctcgtagg acgcctccag ccggtcgccg gtcgcaaggc ccttggcgat atcgccttgg
     1861 gatttggcga ccacgccgtc cttttcgctg gccgcatgca aatcgcgcca gatgtcgttc
     1921 gagctgatcc gcgcgttcgg gccctcatcc cagtctaccg agagcgcatc gaggcctttc
     1981 ttggccgccc acatgtggtc gccgaccacc gcgaccatgt cgtcgagaac gacaatcttc
     2041 cgcacgcccg ggattttcct ggcggcggag tcgtcgacct tgccgacctt gccgccgaat
     2101 accgggcacg ccttgagcgt cgcgaacttc atgtcgggaa gcatggcgtc gatgccgtag
     2161 acggtcttgc cgttgacctt gtcgggtgtg tccaggcgtt tcagcggctt gccgatcagt
     2221 gaaaaatcct tcgcgtcctt cagcgcaacg tcctttggcg gcctctcgct gctcgcgctc
     2281 gtcgccagag cgccatagga aagtttgcgg ccgctgtctt tgtgcacgac ctcgccattc
     2341 gccgtggtgc agctggccgg ctcgacctgc cactcttgcg ccgcagcctg aaccagcatg
     2401 gcccgcgcgg aagcgccggc ctcgcgcagc ggtgtccaaa aagcgcgaac cgacgtagag
     2461 cctccggtcg cctgaatgcc gaacgcggga ttggcgtaca gcttctcgtt cgccggcgca
     2521 tgctcgagcg tcacgcgcgc aaaatcggcg tcgagctctt cggccagaat catcgccact
     2581 gcggtataga tgccctgtcc catctcgacc tggggcatga ccagcgtgat attgccggca
     2641 ttgtcgatcc gaatgaaggc gttgggggcg aacttgccgt tcgtgtcatc aggcggctgc
     2701 accggttcgt ttggcgtcaa cgcgcgcagc ggaagatgga atgcgagcag aaacccgcct
     2761 gccagactac cggtcagcag cacgcggcgt gaaagcccgt ccgggatttt ctctgcgact
     2821 ttcatgatac gctcctacga ctgacgctgc gaagccgcct gcttgatcgc ggcgcgaatc
     2881 cgcacatagg tgccgcagcg gcagatattg cccgccatcg cggcgtcgat gtcggaatcg
     2941 tcaggattgg gcgtggccgc gagcagggcc gcggccgaca tgatctggcc cggctggcaa
     3001 tagccgcact ggaccacctc gagatcgagc caggctttct gcacttttgc accgaccggc
     3061 gtggcgccga cgccctcgat ggtggtgata gcgcggtcgc gcaccgcgcc gaccggcagc
     3121 atgcaggaac gcaccggctt tccgtcgaca tgcaccgtgc aggcgccgca tagcgccatg
     3181 ccgcagccga atttcgtccc ggtcatgccc agcacgtcgc gcaagaccca gagcagcggc
     3241 atgtccgcgg gagcgtcaaa ggacttctgt tcgccattga ttgtcagcgt tgttgccata
     3301 agcgcccctt ccaattcaac ggccgcgggc agcggacgcg ttggctacgt cgcgataggc
     3361 tggctcgcgc cgcggtcagt taagaagcga agatatcctt cgcaatgctg acttgggttg
     3421 tcagaggtcg acgacgatga tcacaaaatc gtgctgaatg accgcacggt tttaggccaa
     3481 ttgatatgag ggccatcatt ccagcatgct cctcttggta accgtcgagc taaagcgcag
     3541 cttccgatgc tgttcgagtc tggagcgacg atacgtcggc aatgccgacc tggatagtcg
     3601 gcgg
//
LOCUS       DATNOI010000714         2719 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_110027,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000714 DATNOI010000000
VERSION     DATNOI010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110027"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /gene="nuoG"
                     /locus_tag="VN968_28410"
     CDS             complement(<1..1179)
                     /gene="nuoG"
                     /locus_tag="VN968_28410"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HXL33192.1"
                     /translation="MTRLIIDGEEIDVPPEFTLLQACEAAGAEIPRFCYHERLSIAGN
                     CRMCLVEIKGGPKPVASCAWGVRDCRPGPKGEPPEISTRSPMVKKAREGVMEFLLINH
                     PLDCPICDQGGECDLQDQAMGYGVDSSRFAENKRAVEDKYLGALVKTSMNRCIQCTRC
                     VRFAAEVCGVPELGATGRGEDMEITTYLETALSSELQGNLVDICPVGALTSKPYAFAA
                     RPWELGKTQSIDVMDGVGSAIRVDTRGREVMRILPRVNEAVNEEWISDKTRHVIDGLR
                     TQRLDRPYIRENSQLYPASWFQAFDAIAAKIARSDGKRIGAIAGDLAAVEEMFALKEL
                     LGKYGSINLAVQGGDAFDAKAGRASYIFNPTIAGIEQADALLIVGSNPRREAAVLNAR
                     I"
     gene            complement(1226..2551)
                     /gene="nuoF"
                     /locus_tag="VN968_28415"
     CDS             complement(1226..2551)
                     /gene="nuoF"
                     /locus_tag="VN968_28415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HXL33193.1"
                     /translation="MLDDKDRIFRNLYGRQDWGLEAARRRGAWDGTKAIIDKGRDWII
                     AEMKASGLRGRGGAGFPTGLKWSFMPKESTDGRPSYLVVNADESEPGTCKDREIMRHD
                     PHLLVEGCLLASFAMGAHVCYVYVRGEFIREREHLQAAVDQAYEARLIGKDNVNGWPF
                     DLYVTHGAGAYICGEETALLESLEGKKGQPRLKPPFPANVGLYGCPTTVNNVESIAAA
                     PDILRRGAAWFAAIGRPNNVGTKLFCISGHVDRPCNVEEAMGIPFRELIERHCGGVRG
                     GWDNLKAVIPGGSSVRMVPADQIIDCPMDFDSLGKLRSGLGTAAVIVMDKSTDLIRAI
                     ARISYFYKHESCGQCTPCREGTGWMWRVLTRMAEGRAHKREIDMLLEVTKQVEGHTIC
                     ALGDAAAWPIQGLIAHFRHEIEERIDEYSRKADVDDVGILDPAHMVAAE"
BASE COUNT          478 a          883 c          850 g          508 t
ORIGIN      
        1 gatgcgcgcg ttgagaacgg cggcctcccg gcgcgggttc gagcccacaa tcaagagcgc
       61 gtcggcctgc tcgatgccgg cgatggtcgg attgaaaatg taggaggcgc gaccggcctt
      121 ggcatcgaaa gcatcgccgc cctggaccgc gaggttgatg gaaccatatt tccccagcag
      181 ctccttgagc gcgaacatct cctccaccgc ggcgagatcg cctgcgatgg cgccgatccg
      241 cttgccgtcg ctgcgggcta tctttgcagc aatcgcgtcg aacgcctgaa accatgacgc
      301 cgggtagagc tggctgtttt ccctgatgta gggacgatcg agccgctgcg tgcgcaggcc
      361 gtcgatgacg tggcgggtct tgtcggaaat ccactcctcg ttcaccgcct cgttgacgcg
      421 cggcagaatc cgcatcacct cgcggccgcg ggtatcgacc cggatcgccg acccgacgcc
      481 gtccatcacg tcgatcgact gcgtcttgcc gagctcccag ggccgcgctg cgaaagcgta
      541 gggttttgag gtcagcgcgc cgaccgggca gatatcgacg aggttgccct gcagttccga
      601 gctcagcgcc gtctccagat aggtggtgat ctccatgtcc tcgccgcggc cagtcgcgcc
      661 cagttcgggc acgccgcaga cttcggcagc gaagcggacg cagcgcgtgc actggatgca
      721 gcggttcatc gaggtcttga ccagcgcgcc cagatatttg tcctcgaccg cgcgcttgtt
      781 ttcggcgaag cggctcgagt caacgccata gcccatcgcc tgatcctgca ggtcgcactc
      841 gccaccctga tcgcaaatcg ggcaatccag ggggtggttg atcagcaaaa actccatcac
      901 gccttcgcgc gccttcttta ccattggcga gcgcgtcgag atttctggcg gctcgccctt
      961 gggaccggga cggcaatcgc gcacgcccca ggcgcagctc gcgaccggct tcgggccgcc
     1021 cttgatctcg accagacaca tccggcaatt gccggcgatc gatagccgtt cgtggtagca
     1081 aaagcgcgga atttcagcgc ctgctgcctc gcacgcctgc agcagcgtaa attccggggg
     1141 aacatcgatt tcttcgccat cgatgatgag tctggtcata gctctctaag tctttctcgc
     1201 cttgcagtgc ggcttggaca tcgcgctact ccgccgcgac catgtgtgcg ggatcgagaa
     1261 tgccgacatc gtcgacatcg gccttgcgcg aatattcgtc gatgcgttcc tcgatctcgt
     1321 gacggaaatg cgcgatcagg ccctggatcg gccatgccgc ggcgtcgccg agcgcgcaga
     1381 tggtgtgacc ctcgacctgc tttgtcactt ccagcagcat gtcgatctcg cgcttgtggg
     1441 cgcggccctc ggccatccgg gtcagcaccc gccacatcca ccccgttcct tcgcggcatg
     1501 gcgtgcactg gccgcaactc tcgtgcttgt agaaatagga aatccgcgcg atcgcccgga
     1561 tcaagtcggt cgatttgtcc atcacgataa ccgcggcggt gccgaggccg gagcgcagtt
     1621 ttcccaggct gtcgaaatcc atcgggcagt cgatgatctg atccgccggc accatccgca
     1681 ccgacgaacc gccggggatc accgccttga gattatccca gccgccgcgg acgccgccgc
     1741 aatggcgctc gatcaactca cggaatggga ttcccatggc ctcttcgacg ttgcaggggc
     1801 ggtcgacatg gccggagatg cagaacagct tggtgccgac attgttgggc cggccgatgg
     1861 cggcgaacca cgccgcgccg cggcggagaa tatcgggcgc ggccgcgatc gattcgacat
     1921 tgttgaccgt cgtcgggcag ccgtagagac cgacattggc gggaaacggc ggctttagcc
     1981 gcggctggcc cttcttgcct tcgagacttt ccaaaagcgc ggtctcttcg ccgcagatat
     2041 aggcgccggc accatgggtc acgtaaaggt cgaacggcca gccgttgaca ttgtccttgc
     2101 cgatcagcct ggcttcatag gcctgatcga ccgcggcctg cagatgctcg cgctcccgga
     2161 tgaattcgcc gcgcacgtag acgtagcaga cgtgagcacc catcgcgaag cttgcgagca
     2221 agcagccctc gactaaaaga tgcgggtcgt gccgcatgat ctcgcggtct ttgcaggtgc
     2281 cgggttcgga ctcgtcggca ttgaccacca gatagctcgg ccggccgtcg gtcgattcct
     2341 tgggcatgaa cgaccatttc agaccggtcg gaaaacccgc gccgccgcgt ccgcgcaggc
     2401 ccgaggcttt catctcagcg ataatccagt cgcggccttt gtcgatgatg gccttggtgc
     2461 cgtcccaggc gccgcggcgg cgcgcggctt cgagacccca gtcctggcgg ccatagagat
     2521 tgcggaagat gcggtccttg tcgtcgagca tgaagtgaag cttccctcgg ggtcagcgat
     2581 tggactgcat gtaggcaagc ccggcggcgc aggcgccgaa ggtcagggcc catagcaggc
     2641 ttgaaccaag cgacgcactc agcccgtagc gctgcagcag gaaaatgaat gcactggccg
     2701 ccacggcgtg cagagcgat
//
LOCUS       DATNOI010000715         8017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1363159,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000715 DATNOI010000000
VERSION     DATNOI010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1363159"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VN968_28420"
     CDS             <1..306
                     /locus_tag="VN968_28420"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HXL33194.1"
                     /translation="VGPDGHTASLFPDYPAIEEIERWVVGVPQAHVEPFVPRVTLTLP
                     ALGSCREMLFEVSGPDKRAILTRVLDGEDLPANRARSTGETIWLADEAALPENFRER"
     gene            338..856
                     /locus_tag="VN968_28425"
     CDS             338..856
                     /locus_tag="VN968_28425"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HXL33195.1"
                     /translation="MGVSGSGKSTIADKLAERLGWTYEDGDKFHPASNVAKMSAGRPL
                     TDEDRWPWLQAIAGEIDRVCKAGQHVVIACSALKHAYRDILVHGRDDVRIIYLKGTPK
                     LISARLARRKGHFMPPGLLASQFKTLEPPDTSENPVTVSIDASVETIVDDIVHQLNPG
                     PADSATPRRNRS"
     gene            853..1662
                     /locus_tag="VN968_28430"
     CDS             853..1662
                     /locus_tag="VN968_28430"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXL33196.1"
                     /translation="MTRIALVVSDVDGTLLTKDKTLTDGARRAARRLHEAGIGFTITS
                     SRPTIGMGFLIEPLQITLPVGPFNGSSIVDPQLNPLEQHLIPAAAAQTSLDLLNEFGV
                     DIWLFTNEKWLTRRPDGEYVAHEKLAIRADPTIVTDFTPYLAVACKIVGASSDADLLK
                     RCETAMQQAVGTQATAIRSQSYYLDVTPPGFDKGTFVQAMAKRQGMSTDAVATIGDMQ
                     NDLAMFAKSGMSIAMGNATEDVKKRATHVTTSNEDEGFAGAIDLILKSNAS"
     gene            1697..1885
                     /locus_tag="VN968_28435"
     CDS             1697..1885
                     /locus_tag="VN968_28435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33197.1"
                     /translation="MTDEELIVYAMREAQLVLARHIERSPQDAEETIALLLETLDRQD
                     VVNATERLCRGYGLRPIR"
     gene            complement(1958..2956)
                     /locus_tag="VN968_28440"
     CDS             complement(1958..2956)
                     /locus_tag="VN968_28440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXL33198.1"
                     /translation="MIRFVAAAILSGWLVLPAMAAGLDAASINNAEYRAKLPSDDRAD
                     AVVVKLQVLLDRARFSPGEIDGKLGENAQKALKAFADAKGLPSDKTLTPEIWKTLAGT
                     GGDPVITEYKISKSDVAGPFLKKLPVKMEQMKDLKALSYTSPREAIAEKFHLSEDLLA
                     ALNPGKKFDQAGETVFVTNVLNKQTRLKVGRIEVDKSLQTVRAFAPSGELVAFFPATV
                     GSEEKPTPSGTLKVVSSDANPNYRYNPDYKFKGVKSKEAFTIMPGPNNPVGSYWIGLS
                     AEGYGIHGTAEPSRVSKSESHGCIRMTNWDIGLLGRSTKKGTPVVFIEEPQGGRKS"
     gene            complement(3058..4860)
                     /locus_tag="VN968_28445"
     CDS             complement(3058..4860)
                     /locus_tag="VN968_28445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HXL33199.1"
                     /translation="MSCRIEDYGLIGDCETAALVGRDGSIDWLCWPAFDSDACFAALL
                     GTSKHGRWRIAPAEPVAKSSRRYWENTLILETRFETTSGVVALIDFMPPRGNASDVVR
                     LVRGVRGRVKLRMELVIRLGFGSDIPWVKRTEDGALLAICGQDMTVLRTPVETRGEDL
                     TTVADFEVGEGETIPFVLTYGPSHLPVPAPINPAQALQDTEDFWTEWCSRCTHEGDNR
                     DLVMRSLITLKALTYQPTGGIVAAPTASLPEKLGGARNWDYRFCWLRDATFTLLALMN
                     SGYTEEASAWHNWLLRAVAGAPANMQIMYGIMGQRRLLEWEADWLPGYEGAQPVRIGN
                     AAHAQLQLDVYGELIDAFHQSRVAELELDEGSWALECAVLEHLAEVWDQPDHGIWERR
                     GDGKHYVSSKVMTWVAFDRGIKSAEKFGFDAPLDRWRTLRDTICRDVCEKGFDATQNA
                     FVESYGSQLLDASVLLLPAVGFLPASDPRVRGTIEAIEKYMLRDGFVLRHDPREVSQE
                     RQPIEGAFLACSLWLADAYVLAGEIAKAQALFDRVVGIANDLGLLAEEFDSGEGRQTG
                     NFPQALTHIALINTAHNLGAAKKPLQKPVMQRSK"
     gene            complement(4891..6849)
                     /gene="malQ"
                     /locus_tag="VN968_28450"
     CDS             complement(4891..6849)
                     /gene="malQ"
                     /locus_tag="VN968_28450"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HXL33200.1"
                     /translation="MDLFTKAKHLGIQTEYIDGQGHRHVTSAAALKIIIDALPVRVPH
                     RFLDQAVVIRSGQPSPTELSQAAVLPLRWKIVAGLEVIAQGETTDRVIVWPSDLAVGS
                     YRLYLTDAASVTEKTPLIVAPPRAFGGDFDRCWLLAVQLYGVRSARNWGIGDFTDLEA
                     LIELASQLGADGVGVNPLHALFDDRPADCSPYSPNSRLFLNALYIDVEKLPELQRSAL
                     AESCDTIARLRASDIVDYAAVAELKLRALRSAFDAFKAGPKTDRHAAFSRFRAERGAM
                     LSRYACFEVLRHKFNKPWWEWPTEWQQPNDTRFAGLHAGPDAIEIEFIKFVQWSADQQ
                     LQACRDRAAALGMKVGLYLDLAVGVQSDGFDAWNEQVAISRHLAVGAPPDALNTAGQN
                     WGLAGFNAAGLEIRSFQPFREMLQASMRYAGAIRLDHVLGLKRLYLVPHGFAADDGVY
                     VQMPFEALLAATAQESMKHRCVVIGEDLGTVPEGFREQMADWGIWSYQVMMFERDDRG
                     SFRDIDHYAANALVTFNTHDLSTYAGWRSFSDLKLKRSLGIDPGESDDARWHALTMLS
                     EVLRHHAIDHHDLYAVVGFLARTKSRLLAISLEDLLGLIDQPNIPGTVNEHPNWRRRL
                     PLAIDGIAAAIDIAALRSATAERTRAAV"
     gene            7049..>8017
                     /locus_tag="VN968_28455"
     CDS             7049..>8017
                     /locus_tag="VN968_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HXL33201.1"
                     /translation="MNKTTQTVESAASGAFHIEEVYPLIDGGRFAVKRIVGERVEVWA
                     DIYRDGHDVAAASLVWRRERDREWRREPMVGHGNDRWSGAFVPDEPGRYVYAIEAWTD
                     EFATWRHGFELKQKAGSDLALDAIEGAGLLTKAQASGPAAAAVILKQCEEYLQTGDAA
                     SLLTADLQDAMAESQFRPDLTRSPLFPLVADRPLARAGAWYEMVPRSQGRIPGRHGTF
                     QDCIARLPDVAAMGFDVVYLTPIHPIGHTNRKGRNNAVTATDSDPGSPYAIGAAEGGH
                     DAVHPELGTLADFRAFVAACKLLEMEVALDFAVQCSPDHPWLKQHPQ"
BASE COUNT         1557 a         2565 c         2424 g         1471 t
ORIGIN      
        1 gtcgggcccg acggccacac cgcatcgctg tttccggact atccggccat cgaggaaatc
       61 gagcgctggg tggtcggtgt gccccaagcc catgtcgagc cgttcgtgcc gcgggtgacg
      121 ctgaccttgc ccgcactcgg ctcctgccgc gagatgctgt tcgaggtgtc cggccccgac
      181 aagcgtgcga tcttgacgcg cgtgctggat ggcgaggacc tgcccgccaa tcgtgcgcga
      241 tccaccggcg agacgatttg gctggcggac gaggctgcgc tgccggagaa tttccgtgag
      301 cgatgagacc agtgaaaacc cctgcgcgct ggtcgtgatg ggcgtgtccg gctcgggcaa
      361 aagcaccatc gcggacaagc tcgccgaacg gctcggctgg acctacgagg atggcgacaa
      421 gtttcacccc gcgagcaacg tcgccaagat gagcgccggc cgccccttga ccgatgaaga
      481 ccgctggccc tggctgcagg cgattgccgg cgagatcgac cgggtctgca aggccggcca
      541 gcatgtcgtg atcgcctgct ccgcgctgaa gcacgcctat cgggacatcc tcgtgcatgg
      601 acgggacgat gtccggatca tctatctcaa gggtacgcca aaattgatca gtgcccgcct
      661 cgcccgacgg aagggccatt tcatgccgcc gggactgctg gccagccagt tcaagacgct
      721 ggagccgccg gacaccagcg aaaaccctgt gaccgtgtcg attgacgcct cggtcgagac
      781 catcgtcgat gatatcgttc accaattgaa tccaggtccc gctgacagcg ccacgccgcg
      841 caggaaccgc tcatgacccg catcgccctg gtcgtctccg atgtcgacgg cacgctattg
      901 accaaggaca aaaccctgac cgatggcgcc aggcgcgcgg cgcggcggct gcatgaggcc
      961 ggcatcggtt tcaccatcac ctccagccgt ccgaccatcg ggatggggtt tctgatcgaa
     1021 ccgctgcaga tcaccttgcc ggtcgggccc ttcaatggaa gttcgatcgt cgacccgcaa
     1081 ctcaacccgc tcgagcaaca tctgatcccc gcggccgcgg cgcaaaccag tctggatctg
     1141 ctcaacgaat ttggtgtcga tatctggctg ttcaccaacg aaaaatggct cacgcgccgg
     1201 cccgacggtg aatacgttgc gcatgagaaa cttgcgatcc gggccgatcc gaccatcgtt
     1261 acggatttca cgccctattt ggcggtcgcc tgcaagatcg tcggcgccag ctcggatgcg
     1321 gacctgctga aacgctgcga aaccgcgatg cagcaggccg tggggacgca ggccacggcc
     1381 atccggtcgc aaagctatta tctcgacgtc acgccaccag gcttcgacaa gggcaccttc
     1441 gtgcaggcga tggccaaacg ccagggcatg tcgaccgatg cggtcgccac catcggcgac
     1501 atgcagaacg atctggcgat gttcgcaaag agcggcatgt cgatcgcgat gggcaacgcc
     1561 accgaggacg tcaagaaacg ggcgacgcat gtgacgacgt cgaacgagga cgaaggcttt
     1621 gccggcgcga tcgatcttat tttgaaaagc aacgcgagct agcccggcga acgtgagcgt
     1681 ccctcacatc cccggcatga ccgacgaaga gctgatcgtc tacgcgatgc gcgaagccca
     1741 actggtgctt gcccggcaca tcgagcggag cccgcaagac gctgaggaaa cgatcgcgct
     1801 gcttctggaa actctcgatc gtcaggacgt cgttaacgcg accgagcgcc tgtgccgcgg
     1861 ctatgggctg cggccgatac gttgatcttg ctccggggta ccggccgcaa tatctctgcg
     1921 tgatcaatcg cacgcgtctg cgcgcgcctg cgtcaagtca gctctttcgg ccgccttgcg
     1981 gttcctcgat gaacaccacc ggtgttccct ttttggtgct gcggcccaac aaaccgatgt
     2041 cccaattcgt catccgaatg caaccgtggg attctgattt gctgaccctg gaaggttcgg
     2101 ccgtgccatg gatgccatat ccttccgccg agagcccgat ccaataggat ccgaccgggt
     2161 tattcggccc cggcatgatg gtgaaagcct ctttggattt gacgcctttg aatttgtagt
     2221 cgggattata gcgatagttc ggattggcgt cggatgagac gaccttgagc gtgccgctcg
     2281 gggtcggctt ctcctcgctt ccaaccgtag ccgggaagaa ggcgacaagc tcgcccgagg
     2341 gcgcgaaagc cctgaccgtc tgaagcgact tgtcgacttc gatccggccg accttcaacc
     2401 tggtttgctt attgagcacg ttggtgacga aaacggtttc gccggcctga tcgaacttct
     2461 tcccggggtt tagagccgca agcagatctt cgctcaaatg aaacttctcg gcgatggcct
     2521 cgcgaggact ggtatagctg agagccttga gatccttcat ctgctccatt ttcacgggca
     2581 gctttttcaa aaacggtcct gcgacatcgc ttttggagat cttgtattcg gtgatgacgg
     2641 ggtcgccgcc cgttcccgcg agcgtcttcc agatctccgg cgtcagggtt ttgtcagacg
     2701 gcaaaccctt ggcgtccgcg aaggctttca gagccttttg cgcgttttca ccgagcttgc
     2761 cgtcgatttc tcccggcgag aatcgagccc ggtccagcag aacctgaagc ttgacgacga
     2821 cggcatccgc tctgtcatcg gagggaagct tggcgcgata ttcggcgttg ttgatgctgg
     2881 cggcatccag gccggcagcc atcgccggca acaccagcca gcccgataaa atcgccgcgg
     2941 ccacaaagcg gatcatcaga catcctccag caataccgtg acgacaagaa gttggaggct
     3001 cgcgagttcc gcaccacgtc aaaatgaacg cgccctccgc gcggagccgg cccgtcgcta
     3061 cttcgagcgc tgcatcaccg gcttctgcag cggttttttg gcggcaccga gattatgagc
     3121 tgtattgatc aatgcgatgt gggtgagggc ctgcgggaaa ttgcccgtct gacgcccttc
     3181 gccggaatcg aattcctccg ccaacaggcc gagatcgttg gcaatcccga ccacgcggtc
     3241 gaacagcgcc tgcgccttgg cgatctcgcc ggccagcaca taggcgtcgg cgagccaaag
     3301 gctgcaggcg agaaatgcgc cttcgatcgg ctgccgctcc tgcgagacct cgcggggatc
     3361 gtgccgcagc acgaatccat cgcgcaacat atatttctcg atggcctcga tggtgccgcg
     3421 aacgcgcggg tccgaggcgg ggagaaaacc gaccgcgggc agcagcagga cgctggcatc
     3481 gagcaactgc gatccatagg attcgacgaa cgcattttgc gttgcgtcaa aacccttttc
     3541 gcagacatcg cgacagatgg tatcgcgcaa cgtccgccag cgatcgagcg gcgcatcaaa
     3601 gccgaacttt tccgcgctct tgatgccgcg atcgaacgcc acccacgtca tcaccttcga
     3661 cgaaacatag tgcttgccgt cgccgcgccg ctcccagatg ccgtgatccg gctggtccca
     3721 cacctccgcc agatgctcca gtaccgcgca ttccagcgcc caggatcctt cgtcgagctc
     3781 cagttcggcg acgcgcgact ggtgaaaggc gtcgatcagt tcgccataga catcaagctg
     3841 cagctgggca tgcgccgcgt tgccgatccg aaccggctgc gcgccctcgt agccgggcaa
     3901 ccagtccgcc tcccattcca gcaggcggcg ctgccccatg ataccgtaca tgatctgcat
     3961 attggccggc gcgcccgcaa ccgcgcgcag cagccagttg tgccaggcgg aggcttcttc
     4021 ggtatagccg gaattcatca gcgccagcag cgtaaaggtg gcgtcgcgta gccagcaaaa
     4081 ccggtaatcc cagtttctcg cgcccccgag cttctccggc agcgaggccg tcggtgccgc
     4141 gacaatgccg ccggtcggtt gataggtcag cgccttcagc gtgatcagcg atcgcatcac
     4201 cagatcacgg ttgtcgcctt catgggtgca acggctgcac cattcggtcc agaaatcctc
     4261 ggtgtcttgc aaggcctgcg ccggattgat cggcgctggg accggtagat gggacggccc
     4321 ataggtgagc acgaagggta tggtttcgcc ttcgcccacc tcgaaatcgg ccaccgtcgt
     4381 caggtcctcg ccgcgggtct cgaccggcgt tcgcagcacc gtcatgtcct gcccgcaaat
     4441 cgccagcagc gcgccatcct cggttcgctt gacccagggg atatcggagc caaacccgag
     4501 ccggatcacc aactccatcc gcagttttac ccggccgcgc acgccacgca ccagccggac
     4561 gacgtcggaa gcattgcccc gcggcggcat gaagtcgatc aacgcgacca cgccgcttgt
     4621 cgtctcgaag cgcgtttcca ggatcagggt attctcccag taccggcgcg aggattttgc
     4681 gaccggctcg gccggcgcga tgcgccatcg gccgtgcttg ctggtgccga gcagcgccgc
     4741 aaaacaggca tcggaatcga acgccggcca gcacagccag tcgatcgatc cgtcgcggcc
     4801 gaccaatgcc gccgtctcgc aatcgccgat cagcccgtaa tcttcaatcc tgcaagacaa
     4861 tttcttttcg ctcgctgaaa atgttgtgcc tcagaccgcg gctcgggtcc gctcggcggt
     4921 cgcgcttctg agcgcggcaa tgtcgatcgc tgcggcaatc ccgtcgatcg caagcggcag
     4981 ccgccgccgc caattcggat gctcgttcac ggtgccggga atattgggct ggtcgatcag
     5041 gcccaacagg tcctcgagcg agatcgccag caggcgggat ttggtccgcg ccagaaagcc
     5101 caccacggca taaagatcgt ggtggtcgat ggcgtgatga cgcaacacct cggacagcat
     5161 ggtcaatgca tgccaccgcg cgtcgtcgct ttcgccggga tcgatcccga gcgaccgttt
     5221 cagcttcaga tcgctgaaag agcgccagcc ggcataagtc gacagatcgt gggtattgaa
     5281 ggtgacaagc gcgttcgccg cataatggtc gatgtcgcga aacgagcccc gatcgtcgcg
     5341 ctcgaacatc atcacctgat acgaccagat gccccaatcg gccatctgct cgcgaaagcc
     5401 ttccggcacg gtgccgagat cttcgccgat caccacgcat cgatgcttca tgctttcctg
     5461 tgcggtcgcc gccagcagcg cctcgaacgg catctgcaca tagacgccgt cgtcggcggc
     5521 aaagccgtgc ggcaccagat aaagccgctt cagcccgagc acatgatcga gccggatcgc
     5581 gccggcatag cgcatcgaag cctgcagcat ctcccgaaac ggctggaacg accggatttc
     5641 cagtccggcg gcgttgaagc cggcgaggcc ccagttctgg ccggcggtat tgagtgcatc
     5701 cggtggtgcg ccaaccgcga gatggcggga aatcgcgacc tgctcgttcc aggcatcgaa
     5761 gccgtcggat tgcacgccga ccgccagatc gaggtagaga ccgaccttca tgccaagggc
     5821 ggcggcgcgg tcccggcaag cctgcaactg ctgatcggca gaccattgaa cgaatttgat
     5881 gaactcaatc tcgatggcat cagggcccgc gtgaagaccg gcgaagcggg tatcgttcgg
     5941 ctgctgccac tccgtcggcc attcccacca cggcttattg aatttgtgcc gcaggacctc
     6001 gaagcaggcg taccgcgaaa gcatcgcccc gcgctcggcg cgaaaccttg agaaggccgc
     6061 atggcgatcg gttttgggac cggccttgaa ggcgtcaaac gccgatcgca acgcgcgcaa
     6121 cttcagttcg gcgacggcgg cataatcgac gatatcgctc gctctcagcc tggcgatggt
     6181 gtcacagctt tcagccaaag cactgcgctg caattccggc agtttttcaa cgtcgatata
     6241 gagcgcattc agaaacagcc ggctattcgg agaatacggg ctgcaatcgg caggacggtc
     6301 atcgaatagc gcatgcaacg gattgacgcc gacgccatcc gctcccaatt ggctggcgag
     6361 ttcgatcaac gcttcgaggt cggtgaaatc cccgatcccc caattgcgcg ccgaccggac
     6421 gccgtagagc tgtaccgcca ataaccagca tcggtcgaag tcgccgccga aagcccttgg
     6481 cggcgccaca atcaacggcg ttttttcggt gaccgaggcg gcatcggtca aatacagccg
     6541 gtacgaaccc acagccaaat ccgacggcca gacgatgacg cgatcggtgg tctcgccttg
     6601 cgcgataacc tcaagacccg ccacgatttt ccagcgcagc ggcaagacag ccgcttgact
     6661 gagctcggtc ggcgatggct gaccggaccg aatcaccacc gcctggtcga gaaaccggtg
     6721 cggtacgcga accggcaatg catcaatgat gattttcagc gccgcggcac tcgtcacatg
     6781 gcggtgaccc tgaccgtcga tatattcggt ctggattcca aggtgtttag ctttggtgaa
     6841 aaggtccatt cggcacgcgg tttgcacgca acggcgagag acgtcgcgaa attctctaga
     6901 tgcaggaaca ggatagccgt gtttaactct ccggcatccc cgccgttccc ggggaaccaa
     6961 tacccgatga gcggtttggt atataggtct gagcgtctct tttcccacaa acgaaacggg
     7021 acgtcttttc catgtcaatg gcatcaccgt gaacaaaaca actcaaactg tcgagagcgc
     7081 tgcgagcggc gctttccata tcgaagaggt ctatccgctg atcgacggcg gccgcttcgc
     7141 cgtgaagcgt attgtcgggg agcgtgtcga agtctgggcc gacatctatc gcgacggcca
     7201 tgatgtggcg gccgcgtcgc tggtctggcg ccgcgagcgg gatcgggagt ggcgtcgcga
     7261 gccgatggtc ggccacggca acgaccgctg gagcggtgcg ttcgtcccgg acgagcctgg
     7321 acgctatgtc tatgcgatcg aggcatggac cgatgaattt gcgacctggc ggcacggatt
     7381 tgagctcaag caaaaagccg gctccgacct ggccctcgac gcaatcgagg gcgcgggtct
     7441 gctcaccaag gcgcaggcca gcggaccggc cgcggccgcc gtcatcctga agcaatgcga
     7501 ggagtatctt cagaccggcg acgccgcgtc gctgctgacg gccgacttgc aggacgccat
     7561 ggccgagagc cagtttcggc ccgacctgac gcgatcgccg ttgttcccat tggtcgcgga
     7621 tcggcctctg gcgcgggccg gtgcctggta cgagatggtg ccgcgcagcc agggccgcat
     7681 accgggccgg cacggcacct tccaggattg catcgcgcgc ttgccggatg tcgcggcgat
     7741 gggattcgac gtggtctatc tcacgccgat ccaccccatc ggtcacacca accgcaaggg
     7801 ccgcaacaat gcggtcacgg ccaccgacag cgatcccggg agtccctacg ccatcggtgc
     7861 cgccgagggc ggccatgatg cggtgcatcc ggaattgggg acgctggcgg atttccgtgc
     7921 gttcgttgcc gcatgcaagc ttctggagat ggaggtcgcg ctcgacttcg cggttcagtg
     7981 ttcgcccgat catccctggc tcaaacagca tccgcaa
//
LOCUS       DATNOI010000716         2670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_681656,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000716 DATNOI010000000
VERSION     DATNOI010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681656"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..939)
                     /locus_tag="VN968_28460"
     CDS             complement(85..939)
                     /locus_tag="VN968_28460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33202.1"
                     /translation="MLPGFRFLLTAIVLSLSMLVLGLGAAALLRAAHEEFAINPSWHA
                     APETTFAQQGEATRPFLALLRVDAPAVDTPKIETPKIETPSTDVPAASAGPAEPAAIS
                     SAPAEPQKVAALKPEESSPPEAAKPAMPALESPSQSEAAPAVADAPATVDQTKIATAA
                     ETLPQANEAAPAATAVAPAAEAAPAAVEQTSAAAAPDPGVAATKIATLGGPPVTIEPQ
                     RAGKLAGAKPDKTVNEKQARRAALRRRMAARARLARQALLLQQAANPFFRPAIQPGVQ
                     PAVQPANR"
     gene            1299..2537
                     /locus_tag="VN968_28465"
     CDS             1299..2537
                     /locus_tag="VN968_28465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXL33203.1"
                     /translation="MMIRHILTIGAAVAAGSAATSLALAQSYPVPPGAVYSTAPAPAP
                     YSPGGYPADYRRAPAAPDFDALEEDDDAPNAQSSTALPPPGPILSPSDPRYGRPAGPP
                     VYTARGAPTGPILSPDDPRYGRPDGPPPVIYSDRPAGPPQQSYSDRGNDDSRIPGSGF
                     IYPNEDNRGLRPPEGIGAPGAVTGTVQPPMGADGKPTVLSALPPDEQPEVAPAQLSPN
                     LRRQEVAFPTKEPAGTLIVDTPNTYLYYVLGGGRAIRYGVRVGRDGFTWTGVQKISRK
                     AEWPDWIPPTEMIERQPYLPRFMAGGPGNPLGARAMYLGSTVYRIHGTNQPSTIGKFV
                     SSGCIGMLNEDVSDLFDRVKIGTRMVVLSGGPTPGTATASAAPPIPGPAGAAPAQMSG
                     IPGGTQPTVVPPLPAPVTVR"
BASE COUNT          420 a          905 c          872 g          473 t
ORIGIN      
        1 ccgcgcttta tgacggctca tgggccgaat ggggcctgcc cgacggaccg ccgcttgcga
       61 ccggcccggc ctgatccgcg ccgatcagcg gttggctggt tggacggccg gctgaacgcc
      121 cggttggatc gcgggtcgaa agaaaggatt ggccgcctgc tgcagcagaa gcgcttgtcg
      181 cgcgaggcgc gcgcgcgccg ccatcctgcg gcgcagggcc gcgcgccgtg cctgcttctc
      241 gttgacggtc ttgtcgggct ttgcgccggc gagcttgccc gcccgctgcg gctcgatggt
      301 gacgggcggg ccgcccagcg tcgcgatttt cgtcgcagcg acgccaggat ccggcgcagc
      361 cgccgcgctc gtctgttcga ctgcggcagg ggccgcttcc gcggcagggg caaccgccgt
      421 ggcagccggt gccgcttcgt tcgcctgtgg caaggtttcc gccgctgttg cgatcttggt
      481 ttggtccacg gtggcgggcg cgtcggccac agctggcgcg gcctcacttt gtgacggact
      541 ttccaaggcc ggcattgcgg gcttcgcagc ttcgggcggc gatgactctt ccggcttcag
      601 cgcggcgacc ttctggggtt cagccggtgc cgaactgatc gcggctggct ccgctgggcc
      661 cgccgatgcc gccggaacat cagttgatgg cgtctcgatt ttgggcgtct cgattttagg
      721 cgtatcgacg gcgggcgcgt cgacccgcag cagcgccagg aaaggtcttg tggcctcgcc
      781 ttgttgcgca aatgtggttt cgggggccgc gtgccatgat ggattgatgg cgaattcctc
      841 atgggccgca cgcagcagcg ccgccgcgcc aagcccaagg accaacatcg acagcgagag
      901 tacgatcgcg gtgagcagaa aacggaaacc ggggagcata gatgcgagtt ccaccttcgc
      961 agggttgctg caaagcttct tgagctttca ggaacgcggt gaccacaaaa tcggcgtgtt
     1021 cacggcgcaa ccgacactcc agcgcgcagc gaatcaggtt gatttgagaa tagcgtaagg
     1081 catccgcatc ggttgttaaa aatcgacccg gcgagtatgc gccctgagag ccggaacagc
     1141 gtttccggcc aggtgaggca tggttgccac ggcgcaaccc gtatcatcac aatgctggaa
     1201 tccggcgcga attggccgga tttgtcttga atgtgccgct atcttccgtc atctgcccgt
     1261 taacggtccg gtgtcggcat ggggactata caacagcgat gatgatcaga cacatcttga
     1321 cgattggtgc tgccgtggca gcgggctccg cggcaacgtc gctggcgttg gcgcagagct
     1381 atccggtacc tcccggcgcg gtctattcga cagcaccggc gccggcgcct tattcgcccg
     1441 gcggctatcc ggccgattat cgccgcgctc cggctgcgcc ggatttcgat gcgctggaag
     1501 aagacgacga cgcgccgaac gcgcagagct cgaccgcgct gccgccgccg ggaccgatcc
     1561 tttcgccctc cgatccgcgc tatggccgcc ccgccggtcc gccggtttat accgcccgtg
     1621 gcgcacccac cggcccaatc ctttcgcctg atgacccgcg ttatggccgc cccgacggac
     1681 cgccgccggt gatctattcg gaccgccccg ccggtccgcc gcagcaaagc tattcggatc
     1741 gaggcaacga cgacagtcgc attcccggat cgggtttcat ctatcccaac gaggacaatc
     1801 gcggccttcg cccgcccgag ggtatcgggg ctccgggtgc ggtcaccgga acagtgcagc
     1861 ctccgatggg cgccgacggc aagccgacgg tgctgtccgc gctgccgccg gatgaacagc
     1921 cggaggtcgc tcccgcgcaa ctctcgccga atctgcgccg ccaggaagtc gcgttcccga
     1981 ccaaggagcc ggcgggaacg ctgatcgtcg ataccccgaa cacctatctc tattacgttc
     2041 tgggcggcgg ccgggcgatc cgctacggcg tccgcgtcgg tcgcgacggc ttcacctgga
     2101 ccggcgtgca aaagatctcg cgcaaggccg agtggccgga ttggatcccg ccgaccgaga
     2161 tgatcgagcg ccagccttat ctgccgcgct tcatggccgg cggccccggc aatccgctgg
     2221 gcgcgcgcgc gatgtatctc ggttccaccg tatatcgcat ccatggcacc aaccagccct
     2281 cgacgatcgg caaattcgtc tcgtcgggct gcatcggcat gctgaacgag gatgtctcgg
     2341 acctgttcga ccgcgtcaag atcggcaccc gcatggtcgt gctttcgggt ggcccgacgc
     2401 cgggaaccgc taccgcctcg gcggcgccgc cgatacccgg acccgccggc gccgcgcccg
     2461 cgcagatgtc gggtattccc ggtggcaccc agcccaccgt cgtgccgccg ctgcccgcgc
     2521 cagtgacggt aaggtaaatc tcgtgtcccg gacgcggtgc aacgctcttg cgttgctccg
     2581 cagagccggg gcccatttgc agttcaaatc gctcgcactt taacaactcg caacaagatg
     2641 gaccccggat cagcagcgca ccacgccgca
//
LOCUS       DATNOI010000717        51923 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_1572720,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000717 DATNOI010000000
VERSION     DATNOI010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 51923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51923
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572720"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VN968_28470"
     CDS             <1..952
                     /locus_tag="VN968_28470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HXL33204.1"
                     /translation="GGADKVVNSIVVRGRDQVMLKVTVAEVRRDIIKQMGVDLSASMN
                     YGTAVVNFNNSNPFTANNAPLVPGNNLTAAALTKAGLPSVTATLRAMESAGVVRTLAE
                     PNLTAISGESATFISGGEFPIPTGVTCQTSTTGAIGNCVQTVSFKKFGISLNFTPVVL
                     TEGRISLRVMTEVSEVSAENSLTGGAGGTTIPSIKTRRAETTLEIPSGGSIAMAGLIQ
                     EQTKQAINGLPGLDSLPVLGALFRSQDFVNNQTELMVIVTPYIVRAVAQKELSRPDDG
                     FAPASDSQSTLLAQINRIYGVAARVEPVGSYQVSYGFIID"
     gene            992..1732
                     /locus_tag="VN968_28475"
     CDS             992..1732
                     /locus_tag="VN968_28475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HXL33205.1"
                     /translation="MTNRTPADRYRTLRLLGALLGLSAALGACANSGGEVVTASVPDD
                     YRLRHPIAIQEADRSVVIFVGHARGGLSASQRADVVGLAQTWAREGTGAIVADVPVET
                     PNARAAAATFREAQALLAAGGVPPRGIRQRHYHPDDPRAFATIRLSYPRIAAVAGPCG
                     LWPEDLGPSMANTDYSENKSYHNFGCANQRNLAAMIDNPSDLEQPRSETPAYTTRRTE
                     AFEKYRKGDPTSTIYPEADKAKLSDTGK"
     gene            1729..2997
                     /locus_tag="VN968_28480"
     CDS             1729..2997
                     /locus_tag="VN968_28480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXL33206.1"
                     /translation="MISIARQNSEELPDIAPPPADDYIAPAPRVSVQAFCETAETAAA
                     VRSAGEDRRLSKAHLSVQMGGMAAAIEAYHTVPTPNVIILETEGRSDILAGLDQLATV
                     CDAGTRVVVIGSVNDAAPYRELVRRGVSDYVIGPVDTLDVVRSICSLFSASEAVAVGR
                     IIAIVGAKGGVGASTVAHNVAWAIARDLALDSVVIDLDLAFGTAGLDYNQDPTQGIAN
                     AVFSPDRLDTAYMDRLLSKCTEHLSLLAAPATLEQVYDFGANAFDSVFDTLRMTTPCI
                     VLDIPHQWSGWTKRALVGADDILIVAEPDLANLRNTKNMLSLLKAARPNDRAPLYCLN
                     QVGMPKRPEIGARGFAKTIESQPIAAIRFDPRLFGTAANNGQMIADLAAKHRTTEMFL
                     QIAQRLTGRGETKKPRGSFLAPILRKLRAR"
     gene            complement(3111..3935)
                     /locus_tag="VN968_28485"
     CDS             complement(3111..3935)
                     /locus_tag="VN968_28485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXL33207.1"
                     /translation="MLQQLLQHYRFAKLLASTASSAILVLGLCSCQTAATSDITGSLG
                     EKTEASRGVDPRREVESYRDRFRANPKDADAALQYAKALRAAGQQAQAVAVLEQATIA
                     HPGNQALLAGYGRALADNGNFQQAFDVLGRAHTPDDPDWRILSAQGTTLDQLGRHDEA
                     RQYYATALKIAPEEPSVLSNLGLSYVLSKDLPKAEEALRRAHGRADADPRVRQNLGLV
                     IGLQGRLAEAESIVRAGRPAEEAAANVAHLRRLLARKDNAHTDSDKIPVAAIGRPI"
     gene            complement(4109..4354)
                     /locus_tag="VN968_28490"
     CDS             complement(4109..4354)
                     /locus_tag="VN968_28490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXL33208.1"
                     /translation="MLANRRQSERRHCRRVAKIQFGTGSLPRDCTITDISEGGLKVIA
                     EYPEIPPQFTIILSTGHPRQCRLAWRIGCEFGAEFID"
     gene            complement(4603..7026)
                     /gene="clpA"
                     /locus_tag="VN968_28495"
     CDS             complement(4603..7026)
                     /gene="clpA"
                     /locus_tag="VN968_28495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HXL33209.1"
                     /translation="MPTFSQSLEQSLHRALAIANERHHQYATLEHLLLSLIDDSDAAA
                     VMRACSVDLDKLRTSLVNYLETEFENLVTDGADDAKPTAGFQRVIQRAVIHVQSSGRE
                     EVTGANVLIAIFAERESHAAYFLQEQDMTRYDAVNYISHGIAKRPGVSEARPVRGVDE
                     ETETKGNEDSKKKGEALDTYCVNLNKKARDGKIDPVIGRNAEINRAIQVLCRRQKNNP
                     LFVGEAGVGKTAIAEGLAKRIVDSEVPEVLAAATVFSLDMGTLLAGTRYRGDFEERLK
                     QVLKELEAHPNAILFIDEIHTVIGAGATSGGAMDASNLLKPALASGTIRCMGSTTYKE
                     YRQHFEKDRALVRRFQKIDINEPTVEDAIAILKGLKPYFEDYHRLKYTNEAIEAAVQL
                     SSRYIHDRKLPDKAIDVIDESGAAQMLVAENKRKKTIGIKEIETTIATMARIPPKSVS
                     KDDAEVLKHLEQTLKRVVFGQDKAIDALSASIKLARAGLREPEKPIGSYLFSGPTGVG
                     KTEVAKQLAASLGVELIRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDGVDQHPH
                     CVVLLDEIEKAHPDLYNVLLQIMDHGRLTDHNGKQVNFRNVILIMTTNAGAADLARQA
                     FGFTRNKREGDDHEAINRQFAPEFRNRLDAIVSFAHLNADVIGMVVEKFVLQLEAQLA
                     DRDVTIELSEPAKAWLVQHGYDEQMGARPMARVIQEHIKKPLADEVLFGKLKGGGHVR
                     VVLVKDEAAGDVAQEKIGFEYVEGPVTPKPEKLPGTRKRTAPRKPKPPGGPSGGSKGP
                     ASRGPLVKV"
     gene            complement(7280..7612)
                     /gene="clpS"
                     /locus_tag="VN968_28500"
     CDS             complement(7280..7612)
                     /gene="clpS"
                     /locus_tag="VN968_28500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_027548135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HXL33210.1"
                     /translation="MSNNENRAGGTGGPATSVIAKVKPKTKRPNLYRVLILNDDYTPM
                     EFVVHVLEKFFQKDVEAATKIMLHVHHHGIGECGVFTYEIAETKVTQVMDFARKHQHP
                     LQCVMEKK"
     gene            8088..8315
                     /locus_tag="VN968_28505"
     CDS             8088..8315
                     /locus_tag="VN968_28505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33211.1"
                     /translation="MTVETPDPTSSVLPRCASGPHNFCMPTAANQMIPAFNSIGTSLS
                     KLHVAKQSGKSNQEKARPQWPGFLISKTAFV"
     gene            complement(8312..8653)
                     /locus_tag="VN968_28510"
     CDS             complement(8312..8653)
                     /locus_tag="VN968_28510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HXL33212.1"
                     /translation="MVKIEDIQQYGKEHLETVVASATGLQNGFQAIASAYGDYTKKSF
                     EDTKSFVEKLSGVKSLDKAMEVQTEYAKSAYETFVAESQKIAGLYTDLAKQTFKPIEG
                     MVAKFAPAAAH"
     gene            9050..10942
                     /locus_tag="VN968_28515"
     CDS             9050..10942
                     /locus_tag="VN968_28515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXL33213.1"
                     /translation="MFRITLASSRILRACIFGVVTTTAAVIFTNDAADARHSRHRHVA
                     RHHQEARESYNPAFSSIIVDGNSGATLSATNPDASRHPASLTKIMTLYLLFERLDAGK
                     MKLDTEMDVSEHASDQAPTKLGLRPGQTIAVEDAIKGLVTRSANDAAVVIAEAIAGDE
                     GDFAKLMTRKARALGMVRTVYRNASGLPDDEQVTTARDQATLGRAIQDRFPRYYRYFS
                     TTAFNYHGHSIRNHNKLLGNVEGVDGIKTGYTRASGFNLVTSMRRGNRHLVGVVLGGH
                     SGGSRDTIMRGLLAENLEKASTKRTVAAITERNSSDANADVAEAAAASRPTQAVQVQG
                     AVQVASAEPAAPQAERSTAPAPASRSIFAAAAAAVPPPPAKTGPAKIEPAPFTNGVIQ
                     TQQISAIPGSSEPMKPVKVKTVQVKAGQMKLASAAGQAEPVTPATNAIPPARQDVPET
                     SSAMVAKTETNRVGVAKSEPVRPEQTRPEMPPQPAGHGTGNGILGVLPASSLAPSSTP
                     QASTSQASTSQVLAYADPAPNAQPQPQPIQQNGAIKPVAAHTGWIIQVGALDSETEAQ
                     ERIEAARNQAKGLLTKADPFTEPVVGKGDKKLFRARFAGLDRDQAEAACRTLKRSDIS
                     CITIKN"
     gene            complement(11162..11893)
                     /locus_tag="VN968_28520"
     CDS             complement(11162..11893)
                     /locus_tag="VN968_28520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HXL33214.1"
                     /translation="MPTLIAGIIAVILLYSLLQMFRAANPVVLARAIKIGGGVVALAV
                     AAFTGIKGELAVAIPIGIFGAGLLGWSPFGASGFGNIGRMFGGSGTQRSPGQTSQVRS
                     QFLDMTLEHDSGELSGQIVAGPYAGHSLGEFDLRQLTAMISGFDAESVALLESYLDRR
                     FPAWRQDTQGNTAGGQGRPATSGKMTDEEAYQILGLQPGAGRDEIGRAHRALMKKLHP
                     DQGGSTYLAARVNEAKDTLLRTHHS"
     gene            12048..13073
                     /locus_tag="VN968_28525"
     CDS             12048..13073
                     /locus_tag="VN968_28525"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXL33215.1"
                     /translation="MSAPKSRVPLDRRTLLRAGAGMAGILATGRAPAFAQTQPKKLVF
                     AHINAIPESAAVAFDWMAKEVNAQSKGALEMQFFGKTLIPQELEIMNAVKSGSIAMGS
                     PAGAAATVFPEMGALLVPYLVKDYASAYAMLNGTIGDKIGKQIEDNYKLKVLCYFDYG
                     FRHFWTSKKPIVEPKDLRGMKIRVQQAKIFGDTINGLGGSAVPMAYGEVVTAAKQGVI
                     DGGDLPIVNMKALKIYEVSKYASLTYHNYGPTNAVMNLDIWNGLSAEQQKLMMDLARA
                     AQEKIREMTESVDNFAKAKSELEPLGMIVVEAKVDEFRKVAQQKIWPAYKSQYGALWD
                     EIEGFKV"
     gene            13148..13645
                     /locus_tag="VN968_28530"
     CDS             13148..13645
                     /locus_tag="VN968_28530"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HXL33216.1"
                     /translation="MLRIVSAVPKFTVTALIILAIVNLLVGVILRYFVGAITNWLDVD
                     PVPFTWVEEVGELSLAWLTLIGAAIGIQSRSHFALAVFVHRLPLTAQLWIHRFNHALI
                     SGVGLLAAWYGFKLCLLNRTLTTPGLEINLAWLYASSVVGGILIAVYGFAMMISPLPQ
                     GEPQH"
     gene            13669..14952
                     /locus_tag="VN968_28535"
     CDS             13669..14952
                     /locus_tag="VN968_28535"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HXL33217.1"
                     /translation="MLLATVAIVFVALILLSMPIVFALGVAGLAGLWIGGYPMQQLSS
                     ALVSGSQSWVLLAIPAFVFAGNLMERCGMSHALVELARALIGWVKGGLGMSVIVVAYF
                     FSDICGSKMAEVSALGSALMPPMTKAGYDRRDSASLIAAGTAMGMLVPPAIFMIVIAQ
                     VTNTSAVALFVAGFIPAVVIMLCLMAVVYIRARQNDWPIDSRPSLKRLGHAALHAAVP
                     MVVPFVILAGFILGIITATEAGAVVAGYAFLAAKLYYRNVSWREMAKIAYDSAILTAA
                     VVFLLAVASVYQYLMGVSGVPQLLGQVLGPLKTHPWLFLIGTAVMSCLFGMVLEGLPA
                     AVVLIPVVFPIAEAMGIDPIHFNIVQTAAVGIGLFLPPMGVGLLMALRFANLSVGQHF
                     RTYVPYMLALFAGLLLIICIPEISLTLPRLAGLTK"
     gene            15204..15551
                     /locus_tag="VN968_28540"
     CDS             15204..15551
                     /locus_tag="VN968_28540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HXL33218.1"
                     /translation="MNRLEAAVKAKGMTVFARIDHAAGATGAGLSLRPAEVLIFGNAK
                     AGTPLMQSVQTIGLDLPLKALAWQDASGKTWLSYNDPAWLAKRHGLSGEAEAAVNIMA
                     GALEAVAKAATTG"
     gene            complement(15711..15965)
                     /locus_tag="VN968_28545"
     CDS             complement(15711..15965)
                     /locus_tag="VN968_28545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33219.1"
                     /translation="MHLTSCPACSKKEHSDAFGALNCPDASENPFIVYRLGQSQTRLG
                     NVDKGVEYLLRAYMLDGEDIFSSDQNGEEFLAILRSRKLV"
     gene            16128..16823
                     /locus_tag="VN968_28550"
     CDS             16128..16823
                     /locus_tag="VN968_28550"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33220.1"
                     /translation="MSVASVCRLGDEKLDIAFIRSHASNACTIRIIEEPSPLKRRCRR
                     DSQQSIGKLRRREYFYSRRELLVVSGRQFAIFSWFAIESVLLHRLYTVTTLPLALRPT
                     MGIQLAPPTVGAVAHLGVNGGVPDLVAHALVGYGLLQALLLLRLLPWIMEQPFAVSYW
                     SFTFGVTALATAPIRMLGHGDSGAIAVLAPYLFAGANIAVGLIAFGTLRLIAQRRLLP
                     QAAPATTRQGVPA"
     gene            complement(16842..17600)
                     /locus_tag="VN968_28555"
     CDS             complement(16842..17600)
                     /locus_tag="VN968_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXL33221.1"
                     /translation="MSGEPTKPKSSGDVSSAQTGKAGGLPEATPSASEDIAAFVAKAR
                     AMSPHAAGARGRLVFALDATMSRQPTWDMACALQADMFREAAALGSLDIRLVYYRGLN
                     ECRATGWISDSAKLAKLMGKIDCRGGNTQIGKVLSEARREAVASAVRAVVFVGDAMEE
                     RVDDLCAKAGELGLLKVPVFLFQEGHDDVAEQAFREIARLTGGAWCRFDPGAAAQLRE
                     LLRAAAAYAAGGREALLRLSKSGSGAAKLLGQMK"
     gene            complement(17731..18582)
                     /locus_tag="VN968_28560"
     CDS             complement(17731..18582)
                     /locus_tag="VN968_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXL33222.1"
                     /translation="MKRGIVVLASLSALVTVAYFTASKWAIRHETLTFNDPARDNRAV
                     AVDVAVRRDKEMQANAGMITLPVAILNHGNTVKFTEYSFLANVFAARGYLAVSIQHDL
                     PTDAPLVTKVGEIYVGRLPQYQRGIANIRFAVDQMKKIEPNADYDRLTMVGHSNGGDI
                     SMYFAKMYPDEIKKVVTLDNLRVPFMTDGRFKILSFRSKDSVFKPDPGVVPNEEVCEK
                     SGITVVQTGYQHTDMSDRGPDDAKNTIQGMLDKFLDDDSPLKPVESNTPKITEPGPVA
                     LYAPVKN"
     gene            complement(18898..19821)
                     /locus_tag="VN968_28565"
     CDS             complement(18898..19821)
                     /locus_tag="VN968_28565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HXL33223.1"
                     /translation="MLMQASQGQSGSAHVVVLGNEKGGSGKSTTALHVAVALLKAGQR
                     VATIDLDCRQQSFTRYINNRRAWARRTGLDLELPVHCCIKLGETMQIADNESSEFQQF
                     MDAVSAVERTFDFIVIDTPGSDSYLMRLAHSMADTLVTPINDSFLDFDVLGTVDPATY
                     SVTGESHYAEMVRDTRRKRRQLDGASTDWIVVRNRLSMLGSRNKQLVADGLKELSFRL
                     GFRSIDGFAERVVYREFFPRGLTALDDLDETTLGTRPSMGHVTAREEVMSLLRQLKLP
                     LDERGRRRAANRAEWFTQVDKPLEVHDIFSA"
     gene            20020..20871
                     /gene="panC"
                     /locus_tag="VN968_28570"
     CDS             20020..20871
                     /gene="panC"
                     /locus_tag="VN968_28570"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HXL33224.1"
                     /translation="MSRSPMIVRTVPALRRALDSIRAKNNTTALVPTMGALHDGHVSL
                     VRQAKRRAKRVIVSIFVNPTQFAPTEDFGSYPRTWKADLARLAAENVDLIWNPDVKTM
                     YPDGFATRILTEGPATAGLEDRFRPHFFGGVTTVVGKLFTQCRPDFAIFGEKDFQQLR
                     VVTRMAGDLDLGVRVIGSRTVRERDGLAMSSRNVYLSPEERLKAPTLYRAMKESASRL
                     RAGDNIEAAMAGGAELIANAGFVLDYYEPRHADTLAPIASAKDGPVRILVAARLGKTR
                     LIDNIAV"
     gene            20987..21754
                     /locus_tag="VN968_28575"
     CDS             20987..21754
                     /locus_tag="VN968_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33225.1"
                     /translation="MAAWSVRAIIFGGLMTFVAAGFAQAQMPQRPPVGGFGSPPDAMI
                     FYVARGAAGACGPGCSDWIAAEGTVQWDTHKRLLAILDRQAGRKLPVVIHSWGESNLN
                     VATSLGRILRDRGIDTTVGSTEAEACNGKPEAECFALKRPGGPLDAKLSLSDARCDLA
                     CVLMLVGGVQRSLPPGTKMILSGMFIRNRLAPNVSDERREGLTVRFGELFRLYLRDMG
                     VETELLDIVDRNSESHRQIELPPAEWIRLRIVTASSL"
     gene            complement(21794..22231)
                     /locus_tag="VN968_28580"
     CDS             complement(21794..22231)
                     /locus_tag="VN968_28580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HXL33226.1"
                     /translation="MPLHLIKLAVGCESVKELKSWVAQRMQAAKQKGLPRHHIHITRM
                     TPKRVEELLAGGSLYWVIRGEIAAREKILAIEPFRDRDGIGRCRLVMQPKVIAVAPRP
                     MRPFQGWRYFTEHAAPPDLGKAAASVAAMPEPLRRELRDLGLL"
     gene            complement(22272..23258)
                     /locus_tag="VN968_28585"
                     /pseudo
     CDS             complement(22272..23258)
                     /locus_tag="VN968_28585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611352.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23426..23629)
                     /locus_tag="VN968_28590"
     CDS             complement(23426..23629)
                     /locus_tag="VN968_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33227.1"
                     /translation="MERALKHKYTVGETVYFTASNVARPAASGTYEVIRLLPTDGDDC
                     QYRIKNSTEAFERVAKESQLDIS"
     gene            complement(23835..24203)
                     /locus_tag="VN968_28595"
     CDS             complement(23835..24203)
                     /locus_tag="VN968_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HXL33228.1"
                     /translation="MGILDSLENSPAFKGALGQLEAAVVPVVLSEVLGNGSQGGLSAI
                     VAKLQQAGLGDLVKSWTGNGQNLPITAEQLQQVLGSDTVKQLAARFNIPVDQLSKVLA
                     QQLPAAVDHASPDGKLPHTA"
     gene            complement(24309..24965)
                     /locus_tag="VN968_28600"
     CDS             complement(24309..24965)
                     /locus_tag="VN968_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXL33229.1"
                     /translation="MALQLVIGNKNYSSWSMRPWLALRANHIVFDEVFIPLYTGDDDK
                     KRILSFTHSGKVPALIDGDVTIWDSLAIIEYLAEGFPEARLWPEDRARRAHARSISAE
                     MHSGFVALRSECGMNLHRPVGAITLSADAHANIARIQQIWIECRERYGKFGPFLFGAF
                     GGADAMFAPVVHRFRTYAIPVAPEAQDYMNTVMSLPAFQEWTTAGLAETLVIERFETV
                     "
     gene            25099..25776
                     /locus_tag="VN968_28605"
     CDS             25099..25776
                     /locus_tag="VN968_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 domain-containing protein"
                     /protein_id="HXL33230.1"
                     /translation="MGAYSVDILAVGFFILEWTVYAVTLEHTAYGRDSLSARMHVYRE
                     VWIRRLLDRETRMVDTQIMASLQNGTAFFASTSLLAVGGALALLRSTSEALAVLGALP
                     INLSPSPALWEIKCVGLILIFVYAFFKFAWAYRLFNYVAILLGAMPPAVQRDTPEAEA
                     HVIRTTGLFEAAGRHFNRGQRAFFFALGYLGWFVSPWVLFVTTALVVIVTWRRQFASD
                     AWRAVGK"
     gene            complement(25880..26680)
                     /locus_tag="VN968_28610"
     CDS             complement(25880..26680)
                     /locus_tag="VN968_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33231.1"
                     /translation="MEPLRFIRSGIAASALGHLSILMVVMVFAEVHPFASVTAEPIAV
                     DIVSPEEAVQVPKMPEPEPKSQGSDAFDPLLKSAPAASPSPQQAASPKPTAPASSQPD
                     RREAAAQPQAPSTSPVRAYIPPEPDLSIKYHVLIGLPVDLPPDGPKDRPGDGFDAPAS
                     KPADVASTLVAEFRRHLKTCSKLPAAVAPSDKIKIKLRVVMTPEGRLAAEPVLIEASA
                     SAKGPALMQSAIGALEACQPYAMLPADRYGEWKVLDLSFTPQDFGGAS"
     gene            complement(26742..27560)
                     /locus_tag="VN968_28615"
     CDS             complement(26742..27560)
                     /locus_tag="VN968_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXL33232.1"
                     /translation="MQFVEANGAKIPAIGLGTWELRGRTCARLVEQALKLGYRHIDTA
                     QVYENEREVGEGLRASRVRRDELFVTTKVWTTHFAPNDLERSTKESLTKLRLSEVDLL
                     LLHWPNPHVPLAETLGALAHVRKLGMARHIGVSNFTVALIDEAVAACSEPLVCDQVEY
                     HPYLDQTKVRDACARHGMAVVAYSPVAKGRIKNDEILVQIGQTHGKTAAQVCLRWLVQ
                     QNVSAIPRTSRIERLSENIDIFDFALSPDEMWLISQMGSTKGRLTDFGFAPKWD"
     gene            complement(27642..27980)
                     /locus_tag="VN968_28620"
     CDS             complement(27642..27980)
                     /locus_tag="VN968_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXL33233.1"
                     /translation="MQRLRLKADGRIVELRDGQEFPLATVTTSAALATIPVPPAEMET
                     FPAVRDLRRRARLTQLQFASRLGVPVETIRNWEQGKRTPRGPARALLAVIAHAPETVF
                     AALATDSSPV"
     gene            complement(28059..29477)
                     /gene="mgtE"
                     /locus_tag="VN968_28625"
     CDS             complement(28059..29477)
                     /gene="mgtE"
                     /locus_tag="VN968_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HXL33234.1"
                     /translation="MADDVDVAHSADAPPVRLPMRDENGEIRSEFVKDIARAIHVADA
                     ALLRAIVAELHEADLGDLIAALEPEDRVSLVELTGADFDFSALNEVDDSVREEILEEL
                     EPETVAEGVRELESDDAVELLESLDEEDQEEILEKLPPLERDALERSLLYPENSAGRR
                     MQTEFIAVPPDWTVGQAIDYMRDTPDLPDRFYEIYAVDKARHWQGAVSLDALLRSRRP
                     VPLADLIDEDRRRVSVLDDQEEVARLFGKYNLVAAPVVDTTDRLVGVITIDDVVDVIE
                     EEADEDLKALGGVTSHEELSDSVWTIAKGRFNWLLVNLATAFLASSVLGFFEGQLEKM
                     VALAVLAPIVASQGGNAATQTMTVAVRALATRELGSNNAFRVVMREGLVGLVNGLAFA
                     VITGIAAVAWFKIPALGVVIGLAITCNLVAGALGGILIPMVLERVRADPAVASGTFVT
                     TITDVVGFFSFLGIATLWFGLK"
     gene            29562..30380
                     /locus_tag="VN968_28630"
     CDS             29562..30380
                     /locus_tag="VN968_28630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXL33235.1"
                     /translation="MTNIDRLKRARKLVNVTGAAVAALIAWTTAAQAADCPRQGTLGT
                     SRILSVDAATTPRVGLKEYPQTLPLEDHEVVLTFDDGPWPPTTPRVLAALARQCVRAT
                     FFLIGKPASEHPDLVRRIAAEGHTIGHHTWLHRSLMQIKPSETTEEIDHGISAVELAL
                     HGVATSTPSTPFFRFPGFETTAATLDLLQSRGIVVFGTDLWASDWDPMTPKHELKLII
                     DRLNIARKGIILFHDPKAQTAAMLPDFLRYLKDNHYRVVHLVPTGPGVDFDGAP"
     gene            complement(30426..30572)
                     /locus_tag="VN968_28635"
     CDS             complement(30426..30572)
                     /locus_tag="VN968_28635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33236.1"
                     /translation="MASAITTKSKGPLTGMIELRTEGGPIEPGIDEDAALALRTDLER
                     FLTA"
     gene            30617..30766
                     /locus_tag="VN968_28640"
     CDS             30617..30766
                     /locus_tag="VN968_28640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33237.1"
                     /translation="MLAGNKPLQIKACARSNGFWHTGEFAVLYAAQFGEAPSMTLARA
                     RQQLR"
     gene            30909..32249
                     /locus_tag="VN968_28645"
     CDS             30909..32249
                     /locus_tag="VN968_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXL33238.1"
                     /translation="MVSKVLVGVRTGQWAALCLGLLTCSIAPIASAADCPGHPDALGT
                     SRTLVVDPKAHPRIGTMQYAETLPLRDHEVVLTFDDGPLPHNSNQILEILASQCVKAT
                     FFTVGSMAQNSPEGVRKLREAGHTVATHTQTHPIGFDKFSPERARQEIDEGIASVTAA
                     LGEGGGLAPFFRPPGLRRSPAIEDYLASLGIQIWSADFPADDWRHIPASRVYDLAMKR
                     LEAKGKGILLLHDIQARTVAALPSILHEMKARGYHIVHVVPATSQQPNTPTEPLQWQL
                     HPPSETVAISHWPTIPNFVYAEAGTLPAPSLPGSGFNDGRPVSLAEPFDRTSRRAHGV
                     LLPPQAPWPRQSLPPLGNAANVLPVPAQNIFEIQEKPGAPVQTVAPSSRHAEQVVPRA
                     ADARASLISVGASVPRRAIPGGPSSGRARRAGVSQIATHRTQLAAGAGRGVLRH"
     gene            complement(32278..32652)
                     /locus_tag="VN968_28650"
     CDS             complement(32278..32652)
                     /locus_tag="VN968_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33239.1"
                     /translation="MKFLGYAYRFLSNFVFLALVYYSLNSMEKYQQRAVLAILMLAYA
                     VMRAATALRSFYFFQRIERLELEARRLAGLTGDGPAAAASRKQIVNDVGVLRRDGEMK
                     SYIDLLFLALVVLLCVSKIVTD"
     gene            32757..32960
                     /locus_tag="VN968_28655"
     CDS             32757..32960
                     /locus_tag="VN968_28655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33240.1"
                     /translation="MNANVVTAKQTDAPFGIDRVPAGRGFGILRPLEHHAFRSRPPAG
                     AAALQDCLERVNPHHRLDFTLFF"
     gene            34368..34973
                     /locus_tag="VN968_28660"
     CDS             34368..34973
                     /locus_tag="VN968_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HXL33241.1"
                     /translation="MAKQLHYGTTAKVFHWLIVALLSVQYPLGWLMPEIHRGAKPSDA
                     LTFHVSLGITILALIVLRLLWRITHPVAPERSLPAWQQLISEAMHWLLYALVLATTMT
                     GWLLASEHGWSISLFFAVSLPMLTSKGSLLASSIGKWHGTMEWALLSLVGAHVAAAMA
                     HTFIFRDRIMQRMLSTNPMIFDTNREGRKAWVKTARDRGVN"
     gene            complement(35062..35718)
                     /locus_tag="VN968_28665"
     CDS             complement(35062..35718)
                     /locus_tag="VN968_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011649712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXL33242.1"
                     /translation="MDKKTLRERIYEEVVRLIVSGELPSGGWVDEKLVINRLRVSRTP
                     FREAIGTLAKGGLIEIKPYRGFYVRSFSRNETADLYELYKQLQCFAVELAVPHMSDRD
                     IGRYENMLDEAVAALRRGDMIIYAMRDREFYEAIARQSGNAALIDALARCALQIQLCG
                     TITSVSREFAERAAHGYDDIIQAFKARDTPRAAFLVRGLVGRAQEAVLSGFPKEAMDQ
                     "
     gene            complement(35822..36619)
                     /locus_tag="VN968_28670"
     CDS             complement(35822..36619)
                     /locus_tag="VN968_28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HXL33243.1"
                     /translation="MKDSDELRLNAYCDGELDPVSAIEFERRMADDESLKAQYDRLLS
                     LRRAVRSIPQYDVPPGLRARIKSTVDADRPGQAGGPGQAGRMRQAGGTRQRSWSFQAL
                     AAAAVFGAVISSSVMLTIARYDLHEDVARQVVAGHIRGQLAPQPFDVASSNRHTVKPW
                     FTSRLPESPQVVDLAAQGFVLLGGRVDVVGHDPVATIVYKHAAHTVSLTTLPPGRSVS
                     DQAIAGYNVRSWSDAQFTYIAVSDLPSADLETFERAFSAGSPAPEVK"
     gene            complement(36635..37303)
                     /locus_tag="VN968_28675"
     CDS             complement(36635..37303)
                     /locus_tag="VN968_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXL33244.1"
                     /translation="MTTHSSVRRSNFRRTELNEVVSKERSDGSDRVSGQQQASGSIVQ
                     NKDDRAVFDEVFLPHMAEAYRLAQWLTGNANDAEDVVQDAALRAFRGIRKFGAVNARA
                     WSLTIVRNTAYSWLMKNRPKSVVFTDDLSVTEQRELEHESPQGTRIETPEEISLSKAD
                     AEDVQQALAQLPAQYREVIVLREINQLNYRDIAEITNVPIGTVMSRLSRARQYLIALL
                     GDRR"
     gene            complement(37337..37717)
                     /locus_tag="VN968_28680"
     CDS             complement(37337..37717)
                     /locus_tag="VN968_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HXL33245.1"
                     /translation="MQIPISQLRLAAARFALDRALVVALLLGPVIGALLAFGAIAAQD
                     AANVITIDNFTFSPKALTVAVGTTIKWVNHDDIPHTVVEKQTTFRSKVLDTDESYSYT
                     FTSAGTFDYFCGLHPHMVGQIIAK"
     gene            complement(37731..38678)
                     /locus_tag="VN968_28685"
     CDS             complement(37731..38678)
                     /locus_tag="VN968_28685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXL33246.1"
                     /translation="MAKKGIDDHGRGLGRREVLECMVWAGTGVLWTVSGGVPHSVGLI
                     GDALAAEPTGFTFLQMSDSHIGFDKAANPDALGTLREAVAKVKAMPVKPAFIIHTGDI
                     THLSKPAEFDNADKVFGETGVQIHYVPGEHDIIDEERGKAYLERYGKGSKGAGWYSYD
                     DHGIHFIGLVNVVDLKGGGLGNLGAEQLAWLEADLKDKSASTPIVVFAHIPLQVIYKE
                     WGWGTDDGTQALALLKRFGSVTVLNGHIHQLAQKVEGNMTFHTALSTAFPQPAPGSAP
                     SPGPMLVPADKLRTLLGLSTVTVAQGNKPLAIIDKTLAG"
     gene            complement(38905..39333)
                     /locus_tag="VN968_28690"
     CDS             complement(38905..39333)
                     /locus_tag="VN968_28690"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HXL33247.1"
                     /translation="MTVRDSTNFAANPGTAFEVSGSRSPSVLHALRLTVLFIALASTP
                     CLAEDSNNAGAGPVATVSMDHTTFIPGEITVAPGTTVTWVNNEAMPHNVADLNKGFRS
                     KILVKDDKFSFTFTTAGEYDYLCLIHPNMKGKVIVKPGAS"
     gene            complement(40705..42411)
                     /locus_tag="VN968_28695"
     CDS             complement(40705..42411)
                     /locus_tag="VN968_28695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXL33248.1"
                     /translation="MTVFELMQEMKKNIYPMGISAREHESRCQGIIIRCSVIVVDSRL
                     VWPRREATLVKKGFISAAAVFGIATAAHAGELSDIQEQSTQLRQQNQALTKRISDLER
                     RQRKLEAQPVAKQPADSMAADYPSYKAEYKKAPPVNDSLTWNGVTLYGLVDLGITYQN
                     HAAPLSSTSGLGLNYLISKNSNGSYFGIGGNALSSSFVGLKGNVELTEGLSGVFNLQT
                     GFNPWSGRLSDGLGSIVQNNGLAVGAQNSFADSAKDGQAFNVAAYAGLSSPIYGTLTY
                     GRQSALTSDGVVNYDPMGNSGAFSLIGFQGATGGGGATENRIFDNSFKYAVNVGPFRA
                     AAEAQLRAGFGGATGNAFQGQIGADYMGLSVDAIFSHVEDAITSAPLPVGTLLSPTLI
                     NPGNGFVAGTVSDNTSFMLLAKYTIGPVKILGGYEHMQFANPKNPLLTGESIVGGYTL
                     GTVNNANFATDKVLQVFWGGVRYAVRPDVDLSVAYYHEEQNSFQGGTPATLNAAHCSN
                     ASLAQCSGQLDAVSFVADYKFAKRFDAYAGIMWSQVSNGLSNGFLQTYSVDPTVGMRF
                     QF"
     gene            42541..43551
                     /locus_tag="VN968_28700"
     CDS             42541..43551
                     /locus_tag="VN968_28700"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HXL33249.1"
                     /translation="MRKQMRSSKMLIALAGVAAMAWTPAHAYEAGAAGWAQKPGITLG
                     GASAGVPPPGLYMIDQAFTYQSNLAGPGNSVINPHGTTTGVPAAVGVTAFVWVPGWTF
                     LGATYDAAWAIPVGMFAAGSPVNVEASGMINTFIAPVELSWKLGDSGFQVKTGFGMYV
                     PDGTVTGVNGLGNVGNAWWSFMPEFVVSYLKDGWNLTANVFEEINTKSTVTQYRSGDI
                     LHAEFTATKTIGKWTVGPVAYYAGQVSDDKSSAFYGGAINVNRYNIWAVGGSLGYNFG
                     PAQLTVWALDEVSANASGGTPQLPPGPLISKGFTAFAQLSYRLWAPDEPVAAPKSPIY
                     HK"
     gene            complement(43793..44248)
                     /locus_tag="VN968_28705"
     CDS             complement(43793..44248)
                     /locus_tag="VN968_28705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33250.1"
                     /translation="MRCLTALLAAALLLAGVQASDAVVRIVEDRGGRIETYVDKYHGV
                     RGSGEMVIIDGFCASACTIVLGAVAHDKICVTSRAKLGFHAAWDPGSNGRRIANLEAT
                     QTLYSMYPFEVRRWIDQRGGLTPHMIFLSGRQLAAMYSPCYLDSRASSR"
     gene            44614..44835
                     /locus_tag="VN968_28710"
     CDS             44614..44835
                     /locus_tag="VN968_28710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33251.1"
                     /translation="MTNFKLLSVGVILSTAIVTPVFAQQAVSEPGMQAFYQSLGVGSQ
                     SSAPSAMASTRNVSYASTPAKRISAKHKM"
     gene            45092..46036
                     /locus_tag="VN968_28715"
     CDS             45092..46036
                     /locus_tag="VN968_28715"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HXL33252.1"
                     /translation="MLAKKMPLDSDTKLPPSAEIEKTLLCEARIEKLINLAFKREVIR
                     KVTDVILPLNDGGCGPAFYCVHAIGGVAADFRHMARMLGPEQKFYGIQTPTDKRNAEF
                     VSSIGSIGQYYVDELVKFQPEGPFFLGGHSVGATVALEMAQQLIARGREVRLLVVFDG
                     WLLNTGADISFLNPLYWFNLLCYLPRWINEVLLVKYSFQGVCRTLVKKFSSRHGVEGV
                     VNLSAFTPEHAAFMRGLYDRHLEYVPNSYPGRVLVFVAKTDELLNHGHVKAAWRKIAP
                     SSEIFEVIGTHVDIMQMPRGLPVAKRLRERIKQLSERA"
     gene            complement(46139..46873)
                     /locus_tag="VN968_28720"
     CDS             complement(46139..46873)
                     /locus_tag="VN968_28720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXL33253.1"
                     /translation="MHNVVVTGGSRGIGLAISRRIAAAGYNVIAVARRESDELRETIR
                     GVVEQGIGGLHFRAFDLSETDAIPAFVKGLRDEFGAIYGLVNNAGIGTEGLLATMHNT
                     EIEALVRLNILSPVILTKYVVRHMMADGAGRIINMSSVIATTGYNGLSVYGATKAAAT
                     GLTRSLAREVGKLGITVNAIAPGFIDTELTQTLSEDQRKRIAGRSALRRLPEVDDIAR
                     MVEYLLGDGGRNITGSVLTIDAGNTA"
     gene            complement(46866..48251)
                     /locus_tag="VN968_28725"
     CDS             complement(46866..48251)
                     /locus_tag="VN968_28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HXL33254.1"
                     /translation="MSSREIFALRDYLSPELKGRTISDARQVWSLTNILEQTCLAGRL
                     GALSGRSVLLAAADQLISAIAMTEIDGVARCMLLCPPDLNADHVNDLIEDAEIDAVVT
                     DQPARWADAGVDLVVADRTPARSAAKSTTEHATDWLMLTSGTSGVPKIVRHTLEGLTG
                     AIAADGPARGAAPVWATFYDIRRYGGLQIFLRAVIGGGSMVLTEPGEPIADHVARLKA
                     REVTHISGTPSHWRKLLMSGSASDFSPRYVRLSGEIADQAVLDGLSRAFPAASIGHAY
                     ASTEAGVGFAVNDGLEGFPATMVGVNRDGVEMKVEDGSLRIRSTRTAHAYVGRNATAL
                     TDGDGFVDTGDMIELRGDRYYFVGRRGGVINIGGLKVHPEEIETVINRHAEVRMSRAR
                     SRRSPITGSIVVADVILADGCDVSRSDDVRNKILADCRASLASHKVPAMIKFVSSLDI
                     TAAGKLARHDA"
     gene            complement(48238..48483)
                     /locus_tag="VN968_28730"
     CDS             complement(48238..48483)
                     /locus_tag="VN968_28730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXL33255.1"
                     /translation="MSVALRIVSVMQQIAKDQQITLPPLNDDVRLHETGFDSLAFAIL
                     VARLEDDLGIDPFTISEDAEFPLTVGDFIKAYENVLA"
     gene            complement(48521..50338)
                     /locus_tag="VN968_28735"
     CDS             complement(48521..50338)
                     /locus_tag="VN968_28735"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HXL33256.1"
                     /translation="MNAGVINPSTDRSRPSGRPSPAKSWLKAIELTSRIEADPRRLFA
                     DLVEDWAKRQPDRPALISEAETFSYRALAERINRYARWALSAGIEAGDTVCLVMPSRP
                     DYLAAWLGITRVGGVVALINTRLVGLSLAHCVNVADADHVILANDLADVFEAARPDLN
                     RAPKIWTHGSRGNEASIDAALDRTDGSPLSPAERRGVTINDRALLIYTSGTTGPPKAA
                     SISHRRILNWGGWFAGLTDASPEDRLYNCLPVHHSVGGIVAPCSMLNAGASVVLSDKF
                     SASTFWQDIVRRDCTLFQYIGELCRYLLKAPPSEYESQHRLRLACGNGLRGDIWEAFQ
                     ARFAIPRILEFYAATEGNFSLYNVEGKPGAIGRIPPLLAHRFPAAIVRVDMDAGTPVR
                     TEDGLCIPCARGEVGEAIGRIGTGDDGGGRFEGYTDAGETEKKILRDVLAKGDAWFRT
                     GDLLRLDEAGFFHFVDRVGDTFRWKGENVATSEVHEAVADCPGVVEAAIYGVAIPGAD
                     GRAGMAAVVVDDTFDLAEFRNRLWRRLPAYACPVMVRFCTALDTTNTFKQKKQQLVRE
                     GFDSRLVTDPLFYLDAKSGGYRPIDADSYKCISNGSLRL"
     gene            complement(50379..50651)
                     /locus_tag="VN968_28740"
     CDS             complement(50379..50651)
                     /locus_tag="VN968_28740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HXL33257.1"
                     /translation="MQIFNANVRDRILDLVKAILKQNSIAVEVDPESRLVNAGLTSMD
                     MVNLMLGVEAEFDFTIPQSEITPENFQSVKTLELMIVGQLKAGTAV"
     gene            50940..>51923
                     /locus_tag="VN968_28745"
     CDS             50940..>51923
                     /locus_tag="VN968_28745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXL33258.1"
                     /translation="MNVQEARLRNAVADRISEQFAPDRSSFVRRAAAVAAAAAEEAED
                     VDRDARFPQKAIDIAREQRLLGIQIPQQFGGDGASIFDIADMCYTLGRACSSTAMIFA
                     MHQTKVACLIRHGAGSGYHEALMRRVASEQLLLASSTTEGQNGGNIRFSTAAVEHAGT
                     EISLVRNATVISYGAQADGIVTIARRANDAAGSDQVLLAITKDDYTLEHSLGWETLGM
                     RGTCSAGFELKFKGSAEQIFPEAYDKIHAQTMTPVAHLCWSSTWAGIAAAAVERAQMF
                     IRKAARGAGGQMPPGAAHFTAARMTLAKLRAMITANLDTYAARQHDERALSS"
BASE COUNT        10216 a        16077 c        15329 g        10301 t
ORIGIN      
        1 gggcggtgcc gacaaggtcg tcaactccat cgtggtccgt ggccgcgatc aggtgatgtt
       61 gaaggtcacc gtcgcggaag tgcggcggga catcatcaag cagatgggcg tcgatctcag
      121 cgccagcatg aattacggca cggcagtcgt aaacttcaac aacagcaatc ccttcaccgc
      181 gaacaatgca ccgctcgtcc ccggcaacaa cttgaccgcg gcggccttga ccaaggccgg
      241 tctgccctcg gtcaccgcta ccctgcgcgc gatggagagc gcgggcgtgg tgcggacgct
      301 tgcagaacca aatctgacgg caatctccgg cgagtccgcg acatttattt caggcggcga
      361 atttccaatt ccgaccggcg tgacctgcca aacgtcgacg acgggggcta tcggaaattg
      421 cgtccagacg gtcagtttca agaagttcgg catctcgctc aacttcacgc cggtggtgct
      481 gaccgagggg cggatcagcc tgcgggtgat gaccgaagtg tcggaagtct cggccgaaaa
      541 ctctttgacc ggcggtgccg gcggaacgac gattccctcg atcaagaccc gtcgcgctga
      601 aaccacgctg gaaattccgt ccggcggctc gatagcgatg gcgggcctga tccaggaaca
      661 gaccaagcag gccatcaacg gattgcccgg cctcgactca ttgccggttc ttggagcgct
      721 tttcaggagc caggacttcg tcaataacca gaccgaactg atggtcatcg tgacgcccta
      781 catcgttcgg gccgtcgccc aaaaagagct gtcgcgtccc gatgatgggt ttgcgccggc
      841 atcggattcg caatcgacat tgttggcgca gatcaatcga atttacggcg ttgccgctcg
      901 tgtcgaaccg gttggaagct accaggtcag ctacggcttt atcattgatt gagaccgaac
      961 tgccccacca atacggtacg aggacagcac gatgacaaac agaacacccg ccgatcgcta
     1021 tcgaaccctg cgcctgctcg gcgcactcct tggcctttcg gccgcgctgg gtgcctgcgc
     1081 caatagcggc ggagaagtgg tgacggcgag cgttcccgac gattaccgcc tgcgtcatcc
     1141 gatcgcgatt caggaagccg accgctcggt cgtcattttc gtcggtcatg cacgtggcgg
     1201 tctttccgct tcacaacgcg ccgacgtcgt gggactggcc cagacatggg ctcgcgaagg
     1261 aaccggcgcg atcgtggccg acgtgccggt cgagacgccg aacgcacggg ctgcggcggc
     1321 tacattccgc gaggcccagg cgctattggc ggcaggcggc gtgcctccgc gcggaattag
     1381 gcagcgtcac taccatccgg acgaccctcg agcgttcgca acgatcaggc tgagttatcc
     1441 aaggattgcg gcggttgccg gaccctgcgg tctgtggccg gaagatctcg gaccctcgat
     1501 ggcgaacacc gactattccg aaaacaagtc gtaccacaat tttggttgcg ctaaccagcg
     1561 caacctcgca gcgatgatcg acaatccgtc ggacctcgag cagccgcggt ccgagacgcc
     1621 tgcgtatacg acgcgacgca cggaagcatt cgagaagtat cgcaagggcg atccgacctc
     1681 gaccatctat cccgaagccg acaaggccaa actcagcgac acaggcaaat gatcagcatc
     1741 gcccgtcaaa attccgaaga actgccggac atcgcgccgc cgcccgcgga cgattacatc
     1801 gctccggcgc ctcgtgtatc ggtacaggcc ttttgcgaga ccgcggagac cgcagccgcc
     1861 gtgcgatcgg cgggcgaaga ccgccgcctc agcaaggccc atctctcggt tcagatgggc
     1921 ggcatggcgg ccgccatcga agcctatcac acggtgccta cgccgaacgt gatcatcctg
     1981 gaaaccgaag gccgcagcga tatccttgcc ggcctcgatc aactcgcgac cgtgtgcgat
     2041 gccggaaccc gcgtcgtcgt gatcggcagc gtcaacgatg ccgcgcctta tcgcgaactg
     2101 gtgcgtcgcg gcgtcagcga ctacgtcatc gggccggtcg atacccttga tgtggtgcgc
     2161 tcgatttgca gtctgttctc ggcatcggag gccgtcgcgg tcggccggat catcgcaatc
     2221 gtcggcgcca aaggcggcgt cggcgcatcc accgtcgcgc acaacgtcgc atgggcgatc
     2281 gcgcgcgatc tcgcgctgga ttccgtcgtc atcgatctcg acctcgcatt cggcaccgcc
     2341 ggtctcgact ataatcagga tccgacgcag ggcatcgcca acgcggtgtt ctcgcccgat
     2401 cggctcgata ccgcatacat ggaccgtctg ctgtcgaaat gcaccgaaca cctcagcctg
     2461 ctggcggcgc cggcgacgct cgagcaagtc tacgatttcg gcgccaatgc cttcgactcg
     2521 gtcttcgata cgttgcgcat gacgacgccc tgcattgtgc tcgacattcc acaccaatgg
     2581 tcgggatgga ccaagcgcgc tctggtcggt gcggacgata tcctgattgt tgccgagccg
     2641 gacctcgcca atctgcgcaa taccaagaac atgttgagtt tgctgaaagc ggcgcgaccc
     2701 aacgatcggg cgccactgta ttgtctcaat caggtcggca tgccgaagcg cccggaaatc
     2761 ggtgcgcgcg gattcgccaa gacgatcgag agccagccga tcgcggcgat tcggttcgat
     2821 ccgcgactgt tcggcacggc ggccaacaac ggccagatga tcgcggacct cgccgccaaa
     2881 catcgcacca ccgagatgtt tctgcagatc gcgcaacggc tgacgggccg cggcgagacc
     2941 aaaaagccgc gcggctcgtt cctggcgccg atcctcagga agctgcgagc aaggtaggcc
     3001 gtcgcgcgga ttgccgcggc gtttcgcaag atgttacgga tcacggccga ccgagcccaa
     3061 agcctcgctg gctgcaagcc tcgctggctg caaggttcgt tcgccgaagg tcagataggc
     3121 cgtccgatgg ctgccaccgg tattttatct gaatccgtgt gtgcattgtc tttgcgagcc
     3181 agcaatcgcc gcagatgagc gacattcgcc gccgcctcct cggccggccg accggccctc
     3241 acaatgcttt cagcctcggc aagacggccc tgcagcccaa tcaccaaccc caaattctgc
     3301 cgcacacgcg gatccgcatc cgcgcggcca tgcgcgcgcc gcagcgcttc ctccgccttg
     3361 ggcagatctt tcgagagaac atatgaaagg ccgagattgg acagcactga aggttcttca
     3421 ggtgcgatct tgagcgcggt cgcgtagtac tgccgggcct cgtcgtgccg gccaagttga
     3481 tcgagcgtgg ttccctgagc cgagagaatc cgccaatcgg gatcgtcggg agtatgggcg
     3541 cggccgagga cgtcgaatgc ttgctggaaa ttgccattgt ccgccagcgc tcgcccgtag
     3601 ccggcaagca gcgcctggtt accgggatga gcgatcgtgg cctgttcgag caccgcgacc
     3661 gcctgggcct gctgccccgc ggctcggagg gcctttgcgt attgcagggc cgcgtcggcg
     3721 tccttggggt tagcacgaaa gcgatcgcga taagattcaa cctcgcgccg agggtcgacg
     3781 ccgcggcttg cttcggtttt ctcgcccagc gaccccgtga tgtccgacgt ggccgcagtc
     3841 tggcagctac aaagccccaa taccaaaatc gcggacgatg ctgtggatgc aagaagtttc
     3901 gcgaagcgat aatgttggag caactgctgg agcatgtact ttgacctggc gtcggcgact
     3961 gaccagaaat gcttcgcagt ttaaccctaa attccagtta aacaggcggg gtgccttggt
     4021 gcgacctgag cgagcccgtc gatatcgact tcgcgcttcc agaccccgag cgttaaccgg
     4081 cctcgatgtt ttgatagagc ttttcgatct aatcgatgaa ttcagcgccg aactcgcaac
     4141 cgatccgcca tgccagccga cattggcgtg ggtggcccgt tgataatatg atggtgaatt
     4201 gaggggggat ttcaggatat tcggcgatta ccttcaggcc gccctccgat atgtccgtga
     4261 tcgtgcagtc gcgcggcaac gagccggttc cgaactggat ctttgcgacc cgtctgcaat
     4321 ggcgacgctc gctctgacgt cgattggcta acatgtcgat ccttcgcgct gctcgataac
     4381 gtccatctgg catttcacct tgcaggaaaa tcgttggaat ctgcctagcg agacctgtcg
     4441 caatgtatct attgtttttg aattccgttt accgggttcc tcggtgatac ctccagcgac
     4501 cgtgaatccg gttacgctgc cggcagcgtc tgctcggcca agcccatcaa aaaagccggc
     4561 ggtattgcga ccgccggctt ttttgttggc aatatcagct actcacacct tgaccagcgg
     4621 accccgtgag gccgggccct tggagccgcc gctgggaccg ccgggtggtt tcggcttgcg
     4681 cggcgcggta cgcttgcgcg tgccgggcag tttttccggc ttcggcgtca ccggcccctc
     4741 gacatattcg aagccgatct tttcctgcgc cacatcccca gccgcctcgt ccttcaccag
     4801 cacgacgcga acgtggccac cgcccttcag cttgccgaac agcacctcgt ccgccagcgg
     4861 cttcttgatg tgctcctgaa tcacgcgtgc catcggccgc gcccccatct gctcatcgta
     4921 gccgtgctgc accagccacg ccttggccgg ctccgacagt tcgatggtga cgtcacggtc
     4981 ggcgagctgc gcctcgagtt gcagcacgaa cttttccacc accatgccga tcacatcggc
     5041 attgagatgc gcgaacgaga cgatggcgtc cagccggttc cggaactcag gcgcgaactg
     5101 gcgattgatc gcttcgtgat cgtcgccttc ccgcttgtta cgcgtgaagc caaaggcctg
     5161 ccgcgcgaga tcggcggcgc ccgcattggt ggtcatgatc aggatcacat tgcggaaatt
     5221 gacctgtttg ccgttatggt cggtcagccg gccgtgatcc atgatctgca gcagcacgtt
     5281 atacaggtcc ggatgcgcct tctcgatctc gtcgagcaga accacgcaat gcggatgctg
     5341 atcgacgcca tcggtgagca ggccaccctg gtcgaaaccg acatagcccg ggggtgcgcc
     5401 gatcaggcgc gacaccgtat gccgctccat atattccgac atgtcgaacc ggatcagttc
     5461 gacgccgagc gacgctgcga gctgctttgc cacctcggtc ttgccgacgc cggtcggccc
     5521 cgagaacaga taggagccga tcggcttttc cggttcgcgc agccctgccc gcgccagctt
     5581 gatcgacgcc gacaatgcgt cgatcgcctt gtcctggccg aacaccacgc gcttgagcgt
     5641 ctgctcaaga tgcttgagca cctcggcgtc gtccttcgac acgctcttgg gcggaatccg
     5701 cgccatggtc gcgatcgtgg tctcgatttc cttgatgccg atggttttct tgcgcttgtt
     5761 ctccgctacc agcatctgcg ccgcgccgga ttcatcgatc acgtcgatcg ccttgtccgg
     5821 caatttacgg tcatggatgt accgcgacga cagctgcacc gcggcctcga tggcctcgtt
     5881 ggtgtatttc agccggtggt agtcctcgaa atacggcttc agacccttga ggatcgcaat
     5941 cgcgtcctcg accgtcggct cgttgatgtc gatcttctgg aaccggcgca ccagcgcgcg
     6001 atccttttca aaatgctggc gatattcctt gtaagtggtt gagcccatgc atcggatcgt
     6061 gcccgacgcc agcgccggct tcaacagatt ggacgcgtcc atggcgccgc cggaagtcgc
     6121 gcccgcgccg atcacggtgt ggatttcatc gatgaacagg atggcgttcg ggtgcgcctc
     6181 gagttccttg agaacctgct tcaaccgctc ctcgaaatca ccgcgatagc gcgtgcccgc
     6241 cagcaacgtc cccatgtcga gcgaaaacac cgtcgccgcc gccaacacct caggaacttc
     6301 gctgtcgacg atgcgctttg ccagcccttc cgcaatcgcg gtcttgccca cgccggcttc
     6361 gccgacgaac agcggattgt tcttctgccg gcggcacagc acctggatgg cgcggttgat
     6421 ctcggcattt cgtccgatca ccggatcgat cttgccgtcg cgcgccttct tgttgaggtt
     6481 tacgcagtaa gtatcgagcg cttcgccttt tttcttggaa tcctcgttgc ccttggtctc
     6541 ggtctcttca tcgacaccgc gcaccggacg cgcttccgac acgccgggcc gcttggcgat
     6601 gccgtggctg atatagttga ccgcgtcata gcgcgtcatg tcctgctcct gcaggaagta
     6661 cgccgcatgg ctctcgcgct cggcgaagat cgcgatcaaa acgttggcgc cggtgacttc
     6721 ctcacggcct gatgactgga cgtggatcac cgcgcgctgg atcacgcgct gaaagcccgc
     6781 ggtcggcttg gcgtcatcgg cgccgtccgt caccaggttt tcaaactcgg tctcgagata
     6841 gttgacgaga ctggtgcgca acttgtcgag atcgacactg caggcccgca tgacggcggc
     6901 cgcatcgcta tcatcgatca gcgacagcag aaggtgttca agcgtcgcat attggtgatg
     6961 acgctcgttc gcaatcgcca gcgcacgatg cagggattgt tcaaggcttt gagaaaaagt
     7021 cggcattcgc gtcctctgtg gcccccgcca tcatgatcgc cgttggcccc tcaggcaaca
     7081 acaacctttg acatatagtt acatccaatc acggcgaaag accggttccc acgacctgtt
     7141 tcttgttttc actccggccg cacacattaa ctcacgcaga gaataagccg ctcccccggg
     7201 aaaaagggcg cacgctcgtc cgatccatcc cgcttttcga tgcaaaaccc gttcctattt
     7261 cgacggattt cgcgcgatgc tacttctttt ccatcacgca ttgcagggga tgttggtgct
     7321 tgcgcgcaaa atccatcact tgcgtcacct tggtctcggc gatctcgtag gtgaacactc
     7381 cgcactcccc gattccatga tgatggacgt ggagcatgat cttggtcgcg gcctcgacat
     7441 ccttctgaaa gaacttctcc agaacgtgaa cgacaaactc catcggcgtg tagtcgtcgt
     7501 tcaggatcaa gacacggtag aggttcggac gcttggtctt gggcttgacc ttggcgataa
     7561 cggatgtggc cggcccgcca gttcccccag cgcggttctc gttgttgctc attcggggag
     7621 caaatgcagc gatggcggat ggcccggaca aatcaaattt gaaagttagc tgcaacatgg
     7681 cacaggcgtt cgaaatcccc acaggaaacc ggacagcgac caagggatgt ctcaagcgat
     7741 gtcctcctgc cggagccatc tgaagagttc ccagacacgc tttccagatc gctgctccgg
     7801 ccggcccgac cccaaccaac cgtatcgatg gttggaatat gggtccgcct ccgacttgcc
     7861 gcaagcgcgc acattttggt ccgatgcggc tggcggcagg ccctgttccg gctcggcgat
     7921 ctgcccgagg ttaatcaatc ctgtcggatt tgacaaagca aagccaccag ccgtttggcg
     7981 aacgttgacg atagaaggtg cttttggggg ccgattcgcg gcctgcacag ctattgaccg
     8041 aattttctcg gggcaggtct accagctgtt cggtcgcgaa agcgggaatg accgtagaaa
     8101 ccccggatcc gacgtcgtcg gtgttgcctc ggtgtgcgag cggtcctcac aacttctgca
     8161 tgccgacggc ggcgaaccag atgatccccg ctttcaactc gatcggaacg tcgctgtcga
     8221 agttgcacgt cgcgaagcaa tccggcaaat ccaaccaaga aaaagcccgg ccgcaatggc
     8281 cgggcttttt gatttctaaa acagcgtttg tttagtgggc ggcggccggg gcgaacttcg
     8341 cgaccatgcc ttcgatcggc ttgaaggtct gcttggcgag gtcggtataa agccccgcga
     8401 tcttctgcga ctccgcgacg aaggtctcgt aggctgactt cgcgtattcg gtctgcactt
     8461 ccatcgcctt gtccagcgac ttcacaccgg acagcttctc gacaaatgac ttggtgtctt
     8521 caaacgactt cttggtgtag tcgccatagg cgctcgcgat ggcctgaaag ccgttctgaa
     8581 ggccggttgc agaggcaacg acggtttcga gatgctcttt tccgtactgc tgaatgtctt
     8641 caattttgac catcatggag ttccttccca gattgatgtg tacttggtgc gccgggaggt
     8701 cctcgctccc tggcatttac tatatagtgc aacgcacaat aaaagtcaag aaatttgtgc
     8761 gacgcacaaa tatcaggatt agtgtcaaat accgcggtct gccgcaaggg ttgattaatc
     8821 ttttggaaac tccagccacc taacctgatt cccggacttg ctcgcgctcc cggcaaacgg
     8881 cctaaaattg tttgtgagca gcatcttggc ttggatggcg gcctgattgg ccatccgctc
     8941 ggagttcagt gccccgatcg ggtgcagcga gcaggcaaac gcgccggccg tcgggcataa
     9001 tttggcctca cgaaccggtg ataaaataga gattgggacg gggaagttca tgtttcgcat
     9061 aaccttggct tcctcgcgga ttctgcgagc ttgtattttc ggggtggtta ccaccaccgc
     9121 tgcggtcatt ttcacaaacg acgctgcaga tgcgcgccac tcccggcacc gacatgttgc
     9181 gcgccaccac caggaagcgc gggagagtta caacccggcg ttctcctcca tcatcgtcga
     9241 cggcaactcc ggcgccacgc tctcggctac caatcccgac gccagccgcc atcctgcttc
     9301 gctcaccaag atcatgacgc tttatctgct gttcgagcgc ctcgatgccg gcaagatgaa
     9361 gctcgacacc gaaatggacg tgtccgagca cgcctcggac caggctccca ccaaacttgg
     9421 cctgcgcccg ggtcaaacca tcgctgtcga agacgccatc aagggactgg tgacccgctc
     9481 cgccaacgac gctgccgtcg tgattgcgga agccatcgcc ggcgacgaag gcgatttcgc
     9541 caagctgatg acccgcaagg cccgtgcgct cggcatggtc aggacggttt atcgcaacgc
     9601 ctccggcctg cccgacgacg aacaggtgac gacggcgcgg gatcaagcga cgctgggccg
     9661 cgccatccag gatcggttcc cgcgctatta ccgctatttc tcgaccacgg cgttcaatta
     9721 ccacggccat tcgatccgca accacaataa actgctgggt aatgtcgaag gcgtcgatgg
     9781 catcaagacc ggctacacgc gcgcgtcggg tttcaatctg gtgacctcga tgcgccgcgg
     9841 caaccggcat ttggtcggtg tggtactggg aggccacagc ggtggctcgc gcgacaccat
     9901 catgcgcggc ttgcttgcgg aaaacctcga aaaagcctcg accaaacgta ccgttgcagc
     9961 aatcaccgag cgcaattcct cggacgccaa tgccgacgtc gccgaggccg cggccgcgtc
    10021 acggccgacc caggcggttc aagtccaggg cgcagttcag gtggcatcgg ccgagccggc
    10081 cgcgccgcag gctgagcgtt cgaccgcgcc ggcaccggct tcacgatcaa tcttcgctgc
    10141 ggctgcagcg gccgtcccgc cgccaccagc caagaccggg cctgccaaga tcgagcccgc
    10201 gccgttcacc aatggggtga ttcaaactca gcagatttcc gcgatccctg gctcgtccga
    10261 gccgatgaag cccgtcaagg tgaagacggt gcaggtcaag gccggacaga tgaagctggc
    10321 ctcggccgcc ggccaggcag agccggttac gcccgccacc aatgcgatcc cgcccgcccg
    10381 ccaggacgtg ccggagacat caagcgcgat ggtggcgaag accgaaacca acagggttgg
    10441 agtcgccaaa tcggaaccgg tcaggcctga acagaccaga cccgaaatgc cgccccagcc
    10501 ggccggccac ggcaccggaa atggtattct cggggtgctg ccggcatcga gcctggcacc
    10561 ctcctccacc ccgcaagctt ccacctcaca agcgtccacc tcacaagtcc tggcctacgc
    10621 cgatccggcc ccgaatgccc aaccgcagcc gcagcccatt caacagaacg gcgccatcaa
    10681 gcctgtcgcg gcccacaccg gctggatcat tcaggtcggc gcactggata gcgaaaccga
    10741 agctcaggaa cggatcgaag cggcgcgcaa tcaagccaaa ggactgctca ccaaggccga
    10801 tccgtttacc gaaccggtcg tcggcaaagg cgacaaaaag ctgttccggg ctcgcttcgc
    10861 cggcctcgac cgcgatcagg ccgaagcggc atgccggacc ctgaagcgtt ccgacatctc
    10921 ctgcatcaca atcaagaact gatcaatcga taagggccaa tatcgccggg agcccggcca
    10981 tatcggccca ttgttgtttt cacgtcggcg cccacgcgca acctctcgtc aagactttgc
    11041 ggctaagact gcaagacgaa cgatcgacca cgccggacaa cggcgggcga gaacagggcg
    11101 tcggccggag tagaaggcag aagcagctcg cgattcgtgg cgtttggtag cgttgtcgga
    11161 gttagctgtg atgcgtgcga agcaaagtat ccttagcctc gtttacacgg gcggcgaggt
    11221 acgtcgaccc cccctgatcg ggatgcagtt tcttcatgag cgcgcggtgc gcacgcccga
    11281 tttcgtcccg ccccgccccc ggctgcaggc caaggatctg ataggcctcc tcatccgtca
    11341 ttttaccgct cgtcgccggg cggccctgcc cccctgccgt gtttccctgc gtgtcctgac
    11401 gccaggcggg aaacctgcgg tccagatagc tttcgagcag cgccacgctt tcggcgtcga
    11461 agcctgagat catcgccgtc aattgccgga gatcaaactc gccaagggaa tggccggcat
    11521 atggcccggc cacgatctgg cctgagagct cgccgctgtc gtgctcgagc gtcatgtcga
    11581 gaaattgcga gcgaacctgt gacgtctgtc ccggcgagcg ttgtgtgcct gagcccccga
    11641 acatccggcc gatattgccg aaccccgacg ccccgaacgg cgaccagccc aatagccccg
    11701 cgccaaaaat cccgatcggg atggcgaccg cgagttcgcc cttgatgccg gtaaaggcgg
    11761 cgaccgcgag cgccaccacg ccgccgccga tcttgatcgc gcgggccaga acgacgggat
    11821 tggccgcgcg aaacatctgc agcagcgagt agagcaggat aacagcgata atcccggcga
    11881 tcagggtagg catgaattca atatagtagg gccggagata aaaagcatgc cggtgatgcg
    11941 cgcaaaacct tttgcccccc aagtagctgc tgtaagcttg ttcccggact ggtcggtcat
    12001 ccctaccggc cgtggactgc aaaaaaggct acccttagag gaacctcatg tcagctccca
    12061 agagccgcgt cccactcgac cgccgcacgc ttctgcgcgc aggcgccggc atggccggca
    12121 ttttagccac cggccgcgcg ccggctttcg cacagaccca gccgaaaaag ctggtattcg
    12181 cgcatatcaa cgcgatcccg gaatcggcag cggtcgcgtt cgactggatg gcgaaggaag
    12241 tgaacgcgca gtcaaaaggc gcgcttgaga tgcagttctt cggcaagacc ttgatcccgc
    12301 aggagctgga gatcatgaac gcggtcaagt ccggcagcat cgcgatgggc agcccggcgg
    12361 gcgccgccgc gaccgtattt ccggaaatgg gcgcgctgct cgtgccctat ctggtgaagg
    12421 attacgcctc ggcctacgcc atgctcaacg ggacgatcgg cgacaagatc ggcaagcaaa
    12481 tcgaggacaa ctacaagctc aaggtgctct gctatttcga ttacggcttc cgccatttct
    12541 ggacatcaaa aaagccgatc gtcgaaccga aggacctgcg cggaatgaaa atccgcgtgc
    12601 agcaggccaa gatcttcggc gacaccatca acggactcgg cggcagcgcg gtgccgatgg
    12661 catatggcga ggttgtcacc gccgccaagc agggcgtgat cgacggcggc gatctgccga
    12721 ttgtcaacat gaaggcgctg aagatctacg aggtgtcgaa atatgcctcg ctgacctatc
    12781 acaattacgg cccgaccaac gcggtgatga atctcgacat ctggaacggg ctgtcggcgg
    12841 agcagcagaa gctgatgatg gatcttgcgc gggccgcgca ggagaagatc cgtgagatga
    12901 ccgagtcggt cgacaatttc gcgaaggcca aatcggagtt ggagccgctc ggcatgatcg
    12961 tggtggaagc caaggtcgac gaattccgga aagttgcgca gcagaagata tggccggcct
    13021 acaagagcca gtacggcgcg ctgtgggacg agatcgaagg cttcaaggtc tgagttggcc
    13081 gcggggcggg gactccagtc tcccgcgccc tcgcccgccc catccatctt ccggaatttc
    13141 ctcgcgcatg ctgcgtatag tttcagccgt cccgaaattc acggtcacgg cgctgatcat
    13201 cctcgccatc gtcaacctct tggtcggcgt gatcctgcgt tatttcgtcg gcgccatcac
    13261 caactggctc gatgtcgacc ccgtgccgtt cacctgggtc gaggaagtgg gcgagctctc
    13321 gctggcgtgg ctgacgctga tcggcgccgc gatcggcatc cagtcgcgct cgcacttcgc
    13381 actcgcggtg ttcgtgcatc gcttgccgct gactgcgcag ctctggatcc accgcttcaa
    13441 tcacgcgctg atatcaggcg tcggtctgct cgcggcgtgg tacggcttca agctctgcct
    13501 cctcaaccgc acgctgacga cgcccggcct cgaaatcaac ctcgcctggc tctacgcgtc
    13561 ttcggtggtc ggcggcatcc tgatcgccgt ctacggtttt gcgatgatga tctccccgct
    13621 gccgcaaggc gagccccagc attgaccagg cactgaggcg gaccattgat gctgctcgcg
    13681 accgtcgcca tcgtcttcgt tgcgctgatc ctgttgtcga tgccgatcgt gttcgcgctc
    13741 ggcgtcgccg gactcgctgg gttgtggatc ggcggctacc cgatgcaaca gctttcctcg
    13801 gcgctggtgt cgggatcgca gagttgggta ctgctggcca tccccgcctt cgtattcgcc
    13861 ggcaatctga tggagcgctg cggcatgagc cacgcgctgg tggaactggc gcgcgcgctg
    13921 atcggctggg tcaaaggcgg gctcggcatg tcggtgatcg tggtggcgta tttcttctcc
    13981 gacatttgcg gatcgaaaat ggcggaagtc tcagcactcg gttcggcgct gatgccgccg
    14041 atgaccaagg ccggctacga ccgccgcgat tcagcctcgc tgatcgccgc cggcaccgcg
    14101 atgggcatgc tggtgccgcc ggcgatcttc atgatcgtga tcgcgcaggt caccaatacg
    14161 tcggcggtgg cgctgttcgt cgccggcttc attccggcgg ttgtcatcat gctgtgcctg
    14221 atggccgtgg tttacatccg cgcgcgccag aacgactggc cgatcgattc caggccgagc
    14281 ctcaagcgcc tcggccacgc cgcgctgcat gccgccgtgc cgatggtggt gccgttcgtg
    14341 atcctcgccg gcttcatcct cggcatcatc accgcaaccg aggccggggc cgtggtcgcg
    14401 ggttatgcct tccttgccgc caaactctat taccgcaacg tctcgtggcg cgagatggcc
    14461 aagatcgcct atgacagcgc gatcctcacc gcggcggtcg tattcctgct cgcggtcgcc
    14521 tcggtctatc aatacctgat gggcgtaagc ggggtgccgc agttgctggg ccaggtgctc
    14581 gggccgctga aaacgcaccc ttggctgttt ctgatcggaa ccgcggtcat gagctgcctg
    14641 ttcggcatgg tgctggaggg cctgcccgcc gcggtcgtgc tcatccccgt ggtgtttccg
    14701 atcgccgagg cgatgggcat cgatccgatc cacttcaata tcgtgcagac cgcggccgtc
    14761 ggcatcggcc tgttcctgcc gccgatgggc gtcgggctat tgatggcgct gcgctttgcc
    14821 aatctgagcg tcgggcagca ctttcgcacc tatgtgccgt acatgctggc gctgtttgcc
    14881 gggctgctat tgatcatctg catccccgag atcagcctga cgctgccgag gcttgcgggg
    14941 ttgaccaagt gagggtccgt ccgaggcagt cgcggaggtg cggccgttca gggcaggcat
    15001 gaaattcaat atcatggggc ttgcgataag aaacatgccg gcggtcccgg cggcccaaaa
    15061 gatcagccag gcgaaaggca cgttggcgtt atcggcacaa attcattatt cttaatcccg
    15121 accgcagccg gatggccatg gagcgcgcaa tggcggcaca gggattgaca acgataccga
    15181 gcagttgtgg gccgcaggat accatgaacc ggctcgaggc cgcggtgaag gcaaagggca
    15241 tgacggtgtt tgcccgcatc gatcatgccg ccggtgcgac cggcgccgga ctgtcgctgc
    15301 ggccggccga ggttttgatc ttcggcaatg ccaaagccgg cacgccgctg atgcaatcgg
    15361 ttcagaccat cggactggac ttgccgctga aggcgctggc ttggcaggat gcatcgggca
    15421 agacatggct ttcgtacaat gatccggcct ggctcgcgaa acggcacgga ctgagcggcg
    15481 aagccgaggc agcggttaac atcatggctg gcgcactgga agccgtcgcg aaagccgcaa
    15541 cgacggggta acccggtgat ttcccgcttc aagccggcga tccgcacgat aggcgataac
    15601 gacaccgtgc catcgcggtt ctcgcgtctt attttttgac ggaagtgtcc gaacgaagtg
    15661 tcacgcggtg agtgtacgaa gacatggtca aggtcggata gcggcaagca ctaaacgagc
    15721 ttcctgctgc gcagtatggc caaaaactcc tcgccattct ggtcggatga aaaaatatcc
    15781 tcaccatcca gcatataggc gcgaagcaga tactccacgc ccttgtccac gttgccaagc
    15841 ctggtctggc tttgtcccag ccgatagaca atgaacggat tttcggatgc gtccgggcag
    15901 ttcagtgcgc cgaacgcgtc cgagtgctcc tttttcgaac atgctggaca cgatgtcagg
    15961 tgcaacgctc tgcttcatgt tctctccttc agacaatctc atccggtttg cttccgaagc
    16021 acctcaatgt tttcccgcgc tcactgcaag cttcctttgt cgctgcatcg ccataaaaat
    16081 tcccgttttc atccatcact ctgacgcagc ggcttgggcc ttcaatgatg tcggttgcgt
    16141 cagtatgccg tctgggcgac gaaaaattag acatcgcgtt tatccgaagc catgccagca
    16201 acgcttgtac aattagaata attgaggagc cctcccccct caagcggcgc tgcaggcgtg
    16261 atagccagca atctattggc aaacttcggc gacgcgaata tttctactct cggcgcgaac
    16321 ttttagtcgt atcgggccga caatttgcga tcttctcgtg gttcgcgatc gagtccgtgc
    16381 tgctgcatcg gctttatacc gtgacgacgc tgccgctcgc attgcggcca accatgggaa
    16441 tccagcttgc tccgccgacg gttggcgcgg tcgcccatct cggcgtcaac ggtggggtcc
    16501 ccgacctcgt cgctcatgcc cttgtcggct atggattgct gcaggcgctc ctgctgctgc
    16561 gcttgctgcc ctggatcatg gagcagccgt tcgcggtgtc ctattggagt ttcactttcg
    16621 gcgtcacagc gcttgccacc gctccgatcc ggatgctcgg ccacggcgac agcggcgcga
    16681 tcgcggttct ggcgccttat ctgttcgccg gcgcaaacat cgccgtcggc ctcatcgcat
    16741 ttgggacctt gcgcctgatc gcgcagcgcc gcttgctccc gcaggccgca ccggcgacga
    16801 cccggcaagg tgtccccgcc tgacaagaac aggaatcggg atcacttcat ctggccaagc
    16861 aatttggcgg cgccgctccc gctcttcgac aaccgcagca gcgcttcgcg tccgccggcg
    16921 gcataggccg ccgctgcgcg cagcagctcg cgcagttgcg ccgcggcgcc gggatcgaac
    16981 ctgcaccacg cgccaccggt caggcgcgcg atctcccgga acgcctgttc ggcgacgtcg
    17041 tcatgcccct cctggaacag gaacaccggc accttgagca ggccgagttc acccgccttg
    17101 gcgcagagat cgtcgaccct ctcctccatg gcgtcgccga cgaacaccac cgcccgcacg
    17161 gccgaggcca cggcttcccg ccgcgcttcc gacaacacct tgccgatctg ggtgttgccg
    17221 ccgcggcaat cgatcttgcc catcagcttt gccagtttcg cgctgtcgga aatccacccc
    17281 gtggcgcggc actcgttgag gccgcgatag tagacaagcc ggatatcgag gctacccagc
    17341 gccgccgcct cgcggaacat atcggcctgc agcgcgcagg ccatatccca tgtcggctgc
    17401 cggctcatgg tagcgtcgag cgcgaacacc agccgacccc tcgcgcccgc ggcatggggc
    17461 gacatcgccc gtgccttggc gacaaaggcg gcaatgtctt cggaagctga cggcgtcgcc
    17521 tcgggcagcc cgccggcttt gccggtttgc gccgaagaca cgtcgccgga agatttgggc
    17581 ttggtcggtt cgccggacat gggcactcgc ggattacagc gagtcttatg tgggccggga
    17641 caagggagcg gtcaatgcgt ggcggcggag ctgattccgg tcagaccgga cgccggcacg
    17701 tttctttcga tcggggcgcg atgcctgccg tcagttcttg accggcgcgt acagcgcgac
    17761 aggtcccggc tcggtgatct tcggcgtgtt gctctcgacc ggtttgagcg ggctgtcgtc
    17821 atcgaggaat ttatcgagca tgccctggat cgtgttcttg gcgtcatcag gaccgcggtc
    17881 gctcatgtcg gtgtgctggt agccggtctg cacgacggtg atgccggatt tctcgcagac
    17941 ctcctcattg ggaacgacgc cgggatcggg cttgaagacc gaatccttcg aacggaacga
    18001 gaggatcttg aatctaccgt cggtcatgaa cggcacccgc aaattgtcca gcgtcacgac
    18061 cttcttgatc tcgtcggggt acatcttggc gaaatacatc gaaatatcgc cgccattcga
    18121 atgtccgacc atggtcagac gatcataatc cgcgttcggc tcgatctttt tcatttggtc
    18181 gaccgcgaag cggatgttgg caatgccgcg ctgatactgc ggcaagcgac cgacatagat
    18241 ttcaccgact ttcgttacca gcggcgcatc ggtcggaaga tcatgttgga tgctgaccgc
    18301 aagatagccg cgggcggcaa acacgttggc caaaaaggag tactcggtga atttgacggt
    18361 attgccatgg ttgaggatcg ccaccggcag cgtaatcatg ccggcattcg cctgcatttc
    18421 cttgtcgcgc cgtacggcga cgtcgaccgc aaccgcgcgg ttgtcgcggg ccggatcgtt
    18481 gaacgtcaga gtctcgtggc ggatcgccca cttgctcgcg gtaaaatatg caactgttac
    18541 cagcgcagaa agcgaagcga gaacgacaat tccacgtttc atatttcgtc ctcggcgcag
    18601 cgaccctggg ggcctaggtt ggggcctatg ttccaatgat cgggtctgtg gtcccttgtc
    18661 catgatacat gtcacgcagg cgtgacagga agagtaaatg ttgtgaagtc accgcctgat
    18721 gattgtgcga cgcaccctct tattgtgcag ttgcacaagg gagggtcggc catcccgctc
    18781 accctgggac ggcacgaatc aaggtttggt tcaggcgtta ggtaaaggca tggtaaaaac
    18841 gtggccgatc aaagatcgtt ccaccgcagg tagtagcctg ctaccgtagg ttttatttta
    18901 ggcgctgaag atgtcgtgga cttcgagcgg cttgtcgacc tgcgtgaacc attcggcccg
    18961 gttcgcggca cggcggcggc cgcgctcatc gagcggcagt ttcaattggc gcagcagact
    19021 catgacctcc tcgcgcgccg ttacgtggcc catgctcggg cgggtgccga gtgtggtctc
    19081 gtcgaggtcg tcgagcgcgg tcagcccgcg cggaaaaaac tcgcgataaa ccacgcgttc
    19141 ggcaaaaccg tcgatcgagc ggaagccgag cctgaacgac agttccttga gaccgtcggc
    19201 gaccagctgc ttgttgcgcg atcccagcat cgacagtcgg ttgcgaacca cgatccagtc
    19261 ggtagaggcg ccatcgagct ggcggcgctt gcgcctggta tcgcgcacca tctccgcata
    19321 atggctttcg ccggtcaccg aatacgtggc ggggtcgacg gttcccagca cgtcgaaatc
    19381 gaggaagctg tcgttgatcg gcgtcaccag cgtatcggcc atcgagtgcg ccagacgcat
    19441 caggtaacta tcggaccccg gggtatcgat aacgatgaaa tcgaaagtcc gctcgaccgc
    19501 gctcacggcg tccatgaatt gctggaactc ggaggattcg ttgtccgcga tctgcatggt
    19561 ctcgccgagc ttgatgcagc aatgcaccgg aagttccaga tcaaggccgg tgcggcgcgc
    19621 ccaggcgcgt cggttgttga tatagcgggt aaaactttgc tggcggcagt cgagatcgat
    19681 ggtggcgacg cgctggcccg ctttcaacag ggccacagca acgtgcaagg cggtggtgga
    19741 tttgcccgaa ccgcctttct cgttgcccag cacaacgaca tgcgcggagc ccgattggcc
    19801 ctgactcgct tgcatcaaca tgatttgacc cctgcgaata tcttcaacta cgcgcgaccg
    19861 cggtcaagtg aaatgcgcaa gcacgcattc gaaacatttg ccgtgtgcct taatgattgg
    19921 actgacggct tggcgacgac gcgttggctt tgataaggtc tcaatccccg ccgcaaggtc
    19981 agaagccgcc gccttcttga ccacacagat cgagtccaga tgtcgcgaag cccgatgatc
    20041 gtccgcacgg tccctgcgtt gcgtcgcgcc ctcgatagca tcagagcaaa aaacaacacc
    20101 acggcgctgg tgccgaccat gggagcgctc catgacggcc atgtctcgct ggttcggcag
    20161 gcgaaacgtc gcgccaaaag ggttatcgtt tcgatcttcg tcaacccgac ccaattcgcg
    20221 ccgacggagg atttcggttc gtacccgcgc acctggaagg ctgatctcgc aaggctcgcc
    20281 gcggaaaatg tcgacctgat ctggaatccg gacgtcaaga ccatgtaccc ggacggcttc
    20341 gccacccgga tattgaccga ggggccggct acagcgggcc tggaggatcg ttttcggccg
    20401 cacttcttcg gcggcgtcac taccgtggtc ggcaagctgt tcacccagtg ccggccggat
    20461 ttcgccatct tcggcgaaaa ggatttccag caactgcggg tcgtgacccg gatggccggg
    20521 gatctcgacc tcggcgtcag ggtgatcggc tcgcgaaccg tgcgcgagcg cgatggcctc
    20581 gcgatgtctt cacggaacgt ctatttgtcc ccggaagagc ggctgaaagc accgacgctg
    20641 tatcgcgcca tgaaggaaag cgcctcgcgt ttgcgtgcgg gcgacaatat cgaggctgcg
    20701 atggccggcg gcgctgaact gatcgcgaac gccggcttcg tcctcgacta ttacgagcct
    20761 cggcacgccg ataccctcgc gccgatcgcc tcggcgaagg acgggccagt gcgaatcctg
    20821 gttgcggcca ggcttggaaa gacacgcctg atcgacaaca tcgcggtcta acgcagcgcg
    20881 gtagcgacgg tcgaccttgc gccggaacaa gggaccatga ccccgcttgc gcaatgcgac
    20941 gccgggacgg aatatcgccg gggacataac ctggggagca gccagcatgg ccgcttggtc
    21001 agtacgggct atcatttttg gcgggctgat gacctttgtc gctgccggtt ttgcgcaggc
    21061 gcagatgccg cagcggccgc cggtcggcgg cttcggttcg ccacccgacg ccatgatctt
    21121 ttatgtcgcg cgcggcgcgg ccggcgcgtg cgggccaggc tgctcggatt ggattgccgc
    21181 cgaaggaacc gttcagtggg atacccacaa gcggctgctt gcgattctgg accggcaggc
    21241 cggacgcaag cttcccgtcg tcatccattc ctggggcgag tccaatctca atgtcgcgac
    21301 cagccttggc cggatcctgc gcgaccgcgg catcgacacg acggtggggt caaccgaagc
    21361 cgaagcctgc aatggaaaac ccgaagcgga atgcttcgcg ctcaagcgtc cgggtgggcc
    21421 gctcgacgcg aagctgagct tatcggacgc gcgctgcgac ctcgcctgcg tgctgatgct
    21481 ggtcggcggg gtccagcgca gcctgccgcc cggcaccaaa atgatcttga gcggcatgtt
    21541 catccgcaac cggttggcac ccaatgtatc cgacgaacga cgcgaaggtc tcacggtgcg
    21601 atttggcgaa ctgttccgcc tgtacctgcg cgacatgggc gtcgaaacgg aactgctcga
    21661 catcgtcgac aggaattcag aatcccaccg tcagatcgaa ttgccgccag ccgaatggat
    21721 ccggctgcgg atcgtcactg cgtcgtcgct ttaactcgag cccgtggcag aaaggcaaaa
    21781 cggggcataa gcgctaaagc agcccgagat cgcgcaattc gcggcgcaac ggctcgggca
    21841 tcgccgcgac gctggcggcg gccttgccga gatccggtgg cgcggcatgt tccgtgaaat
    21901 agcgccaacc ctggaacgga cgcatcggcc gtggcgctac ggcaatcact ttgggctgca
    21961 tcaccagccg gcatcgtccg atcccgtcgc gatcacggaa cggctcgatg gcgaggattt
    22021 tctcgcgggc cgcgatttca ccccggatca cccagtacag tgagccgccc gccagcaatt
    22081 cctcgacgcg cttcggcgtc atccgggtga tgtgaatatg atgacgcggc aagccctttt
    22141 gcttggcggc ttgcatgcgc tgggcgaccc agctcttgag ttccttgacg gattcgcagc
    22201 cgacggcgag cttgatgaga tgaagcggca tggttttcca agttagcggt cagcgacggc
    22261 ttttgaaagc gtcaatcagt cgacgccgca ggcgcggccg tgggcgcaag ctgaaccggc
    22321 gcggaggagg tgcgcgaggg cttcggcgcc gattgctttt tggccgccgc cagcgcagcc
    22381 ggggctgcac tcgaagtcgg gcgcggtgac agcaatgccg cgttcgccgc cgatgctgtc
    22441 ggcgatgccg gcggcgatac cgccgcgcgc ggcggggatg gcgtggccgc cgaacccggc
    22501 tccggggtca tgatgctgac ggccggagtc gaggccgccg tgggaaattc ggcgttggtg
    22561 ggtttgccgg tgggtaccga tggcgctagc gcggcgaccg gcgctgcggc cgcgggcgcg
    22621 ttccatgacg ccgcgtaacg cgagaccaaa ccgtcataag tccgcccggt catgttggcc
    22681 gcgatctagt gggtatccgt cagcgcccgg aaagccgcgc attgcgacgg cgtacattgg
    22741 tcccgcgcca ccagcacctg cgctatcagc ccataccggt cgtgctcgat cgcacggcgt
    22801 agcgcaagca actcgtagct caagttcctg ttggctgcgg cggcatcgcc gaacgcggtc
    22861 agccgggtga cctgagaggc cgcataggat acggccgcgg cggtggattc ggcggaaccg
    22921 aacaatgctt tttcgcacgc cgtcaaaacg gtttcccccg ccagatcgtc gatacacgac
    22981 aatgccggca gcacggcgct cgtctgctgg gaggatcggg tttcgccgga cgcgccgcgg
    23041 tcgcccggac cgaacccgcg catcgtggcg acggctgcga tgccgatggc cagcaacgtg
    23101 attactgtca gtgcgccatt ggcgacggat ttctcggcgc gcaacagcgt aatcagcagg
    23161 atgatcccga agaatgccgc tgcggccaaa gtcagcccca tcgggaaggt cggcgaaccc
    23221 cagagctgat cgagcgaagt agtccaggcc cagttcatgc gcggtgtccc tcaacgcggc
    23281 agcgaacgca ctcggcggaa gtgaatgccg attttcccgc attcagcttc cgcatagtgc
    23341 tcctgcgaac cgggtctttt gacgacgagc gaaatggagc cgccatccgg ccatgtgcgc
    23401 aactcttata ggagttgtcg attcttcagg agatgtcgag ctgactttcc ttggctaccc
    23461 gttcgaaagc ctcggtcgaa tttttaattc gatactgaca gtcatcacca tcggtcggaa
    23521 gcagccggat cacctcataa gtgccgctgg cggccggacg ggcgacattg ctggcagtaa
    23581 aatagacagt ttctccaacg gtatatttgt gcttcaacgc cctctccatc acgcaaaagc
    23641 gtcggccatg cccacggtcc cactgctacg gccgactgaa aaccccgggg gcagcctata
    23701 tcacaggccc gatcacgttg gccagttaca tccggacata gccagtacga gaattttgct
    23761 ggtttttcag tacgataagc gctagtccgg cgatcgcggg ggaatttgcg tcgtcggcgg
    23821 aaaccgtccc ctcgctaggc cgtgtgaggg agcttgccat cggggcttgc gtgatccacc
    23881 gccgcgggaa gctgttgcgc cagcactttt gagagttgat caaccgggat attgaaccgc
    23941 gcggccaatt gcttgacggt gtcgctgccc agcacctgct gcaattgctc ggcggtgatc
    24001 gggaggttct gtccgtttcc ggtccaggat ttgaccaggt cgccgaggcc tgcctgctga
    24061 agcttggcga cgatcgcgga gagcccgccc tggctgccgt tgccgagtac ctcgctcagc
    24121 acgactggaa ccacagccgc ttcgagctgg ccgagtgcgc ccttgaacgc cggcgaattt
    24181 tccagtgaat cgagaattcc catcgccaaa cccttttgct aattgtgcaa cgccggtttg
    24241 cggattcaga ccgctttcgc tcgcgtccgt caagccgcga tgatggccaa tatcttgtca
    24301 tccagacttc acacggtctc gaatctctcg atgacgaggg tttcggcaag gcccgcggtg
    24361 gtccactcct gaaaggccgg cagcgacatc acggtattca tgtagtcttg cgcttcgggc
    24421 gccactggaa tcgcataggt gcgaaagcga tgcaccaccg gtgcaaacat cgcgtccgca
    24481 ccgccaaagg ctccgaacag gaacggcccg aacttgccgt agcgctcgcg gcactcgatc
    24541 cagatttgct gaatgcgcgc gatgttggcg tgtgcatccg ccgacaacgt tatggcgccg
    24601 accggccgat gcaggttcat gccgcattcg ctgcgcagcg cgacaaagcc agaatgcatt
    24661 tccgccgaaa tcgaccgcgc atgggcgcgg cgagcgcggt cctcaggcca tagccgcgct
    24721 tccggaaaac cttcggcgag gtattcgatg atggccagcg aatcccagat cgtgacgtcg
    24781 ccatcgatca gcgcggggac cttgcccgaa tgcgtgaagc tcaagatccg cttcttgtcg
    24841 tcatcaccgg tatagagcgg aatgaaaact tcgtcgaaga cgatgtggtt ggctctgagc
    24901 gccagccagg gccgcatcga ccatgacgaa tagttcttgt tgccgatcac gagttgcaaa
    24961 gccatgcgag aagatccttg ttcgcttgtc atcctgccat agcgcgcgtc aagcgttcaa
    25021 cccgccgaga ggccttcctc gcggttgccg tgatcgatct tgcatgccat gcatttcaca
    25081 accatcgggg gatgcaacat gggtgcatat tcggtcgata ttctggcggt cggcttcttc
    25141 atcctcgaat ggacggtgta cgcggtcacg ctggagcata cggcctatgg gcgcgacagc
    25201 ctgtccgccc gaatgcatgt ctatcgcgag gtttggatcc ggcgcctgct cgatcgcgag
    25261 acccggatgg tcgacacgca gatcatggcg tcgctgcaga acggcacggc cttttttgcc
    25321 tccaccagcc tgctcgcagt cggcggtgcg ctggccctgc tgcgatcgac cagcgaagca
    25381 ctggccgtcc tcggcgcatt gccgatcaat ctcagcccct cgcccgcgct ttgggaaatc
    25441 aaatgcgtcg gcctgatcct gatctttgtc tacgccttct tcaagtttgc ctgggcctac
    25501 cggttgttca attacgtggc catcctgctc ggggcgatgc cgccggccgt gcagcgcgac
    25561 acgccggagg ccgaagcgca tgtcatccgg acgacagggt tgttcgaggc cgcgggccgg
    25621 catttcaatc gcggccagcg cgcgttcttc ttcgcgctgg gctatctcgg ctggtttgtc
    25681 agtccctggg tcttgttcgt cacgacggcg ttggtcgtga tcgtcacctg gcgccggcag
    25741 ttcgcctccg atgcctggcg cgcggtgggg aagtagggcg tttcacgcga agcatgccct
    25801 cggacttgat ccgggggtgg ataccggttc gcgtggagaa aacgcgtcaa atagcaagat
    25861 ggagcggctt ccagccgccc taagacgcgc cgccaaaatc ctgcggcgtg aagctgaggt
    25921 cgagcacttt ccattcgccg tatctgtccg ccggcagcat cgcgtagggc tgacaggcct
    25981 ccagcgcgcc gattgcgctt tgcatcagcg ccggcccctt cgcagacgca ctggcctcga
    26041 tcaagactgg ttccgccgcg agcctccctt ccggcgtcat gaccacccgc agcttgatct
    26101 tgatcttgtc cgagggtgcc accgctgcgg gcagcttcga gcaggttttg aggtggcgcc
    26161 gaaactccgc gacgagagtg gatgccacgt cggccggctt tgacgccggg gcgtcgaagc
    26221 catcaccggg tctgtctttg ggcccatccg ggggcagatc cacgggcagg ccaatcagca
    26281 cgtgatactt gatcgaaaga tcgggctccg gaggaatata ggcgcgaacc ggcgatgtcg
    26341 atggcgcttg cggctgcgct gcggcctctc gccgatcggg ctgcgatgaa gccggtgccg
    26401 tcggcttcgg cgatgcggcc tgctgcggcg acggtgaagc cgcaggtgcg gatttcaata
    26461 gaggatcgaa ggcgtcagag ccttgcgatt tcggctccgg ttcgggcatt ttcggaacct
    26521 gcacggcctc ttcgggcgaa acaatatcaa ccgcgatcgg ctcggcagtg accgaagcga
    26581 acggatgcac ctcggcaaag accatgacca ccatcagaat tgacagatgg ccaagcgccg
    26641 atgccgcgat gcccgagcgt atgaaccgaa ggggttccac cgtatttgat cgcttcgatg
    26701 ccgcctctcg ccggcagcat agcgggcaac ggcaccgcgt ctcaatccca tttcggcgcg
    26761 aaaccgaaat cggtgagccg gcctttggtg ctgcccatct gggatatcag ccacatctcg
    26821 tcgggcgaca gcgcgaagtc gaaaatgtcg atgttttccg acagccgttc gatccgcgac
    26881 gtccgcggta tcgcggaaac attctgctgc accagccagc gcaggcagac ctgcgcggcc
    26941 gttttgccgt gggtttgtcc gatctgcacc agtatctcgt cgttcttgat acgccccttc
    27001 gcaacaggac tataggcgac aacagccatg ccgtggcgcg cgcaggcatc ccgcaccttg
    27061 gtctggtcga gatagggatg gtattccacc tgatcgcaga ccaggggctc cgaacaggcg
    27121 gcaaccgcct cgtcgatcaa tgccacggtg aagttcgaga cgccgatgtg cctggccatg
    27181 ccgagcttcc tgacatgggc cagcgcgccc aacgtctcgg ccagcggcac atgcggattt
    27241 ggccaatgca gcagcaacag atcgacttcg gaaagccgca gtttggtcag gctctccttg
    27301 gtggatcgtt ccagatcatt cggtgcaaaa tgggtggtcc agactttggt cgtcacgaac
    27361 aattcgtcgc gcctgaccct cgaagcgcgc aggccctcgc cgacctcgcg ttcgttctca
    27421 tagacctgcg cggtgtcgat gtggcgatag cccagcttca gcgcctgctc gaccaggcgc
    27481 gcgcaggttc gcccgcgcag ttcccaggtg ccgagcccga ttgcggggat tttagcgcca
    27541 ttggcctcta cgaattgcat cgcggattac ccgattatac caaatcgttc caatatgtcc
    27601 cttgggcgtc gcgctgccaa catcgcgggc tgcagtttcg ctcagactgg cgaagagtcg
    27661 gttgccagag cagcgaaaac cgtctcgggg gcatgggcga tcacggcgag aagcgctcgc
    27721 gccggtccgc gcggcgtgcg tttaccctgc tcccagttgc ggatggtctc aacggggacg
    27781 ccgagccggc ttgcgaattg cagctgcgtc aggcgggcgc ggcggcgaag gtcacgcacg
    27841 gccgggaacg tttccatctc cgccggcggg acgggaatag tcgcgagcgc cgcggatgtc
    27901 gtcaccgttg cgagcggaaa ctcctgcccg tcccgcagtt cgacgatccg tccatccgct
    27961 ttcagccgca agcgttgcat tcccgactcc gcacgggaat agtcggcgaa gcgcgttaag
    28021 gcttgattaa cgatgcgcgg cccgagggcg gcatcgttct atttcagacc gaaccatagc
    28081 gtggcgattc cgagaaacga aaagaagccg accacgtcgg taatggtcgt gacaaaggtt
    28141 cccgacgcca ccgccggatc ggcgcgcacc cgctccagca ccatcggaat cagaatgccg
    28201 ccaagcgcgc ccgccaccag attgcatgtg atcgcgagcc caatcacgac gccaagcgct
    28261 ggaatcttga accaggccac ggcggcgata ccggtgataa cggcaaatgc gaggccgttg
    28321 acgaggccga ccaggccctc gcgcatcacc acgcgaaatg cgttattgga tccgagctca
    28381 cgagtagcca aagcgcgcac cgcgaccgtc atggtctggg tcgcggcatt gccgccctgg
    28441 ctggcgacga tcggcgccag caccgccaac gccaccatct tttccagttg accttcgaaa
    28501 aagcccagca ccgaggacgc cagaaaggcg gtggcaagat tgaccaggag ccagttgaag
    28561 cggcccttgg cgatggtcca gacactgtcg gataattctt cgtggctggt gacgccgccg
    28621 agcgccttca gatcctcgtc ggcctcttcc tcgatcacgt ccaccacgtc gtcgatggtg
    28681 atgacgccga ccagccggtc cgtggtgtcg acgacggggg ctgcgaccag attgtatttg
    28741 ccgaacagcc gcgccacctc ttcctggtcg tccaacacgg agacgcggcg gcggtcctca
    28801 tcgatcaagt cggcaagcgg aaccggcctg cgcgaacgca acagtgcgtc cagcgatacc
    28861 gcgccctgcc agtgccgggc cttatcgacc gcgtagattt cgtaaaaccg gtccggcaaa
    28921 tccggcgtgt cgcgcatgta gtcgatcgcc tgccccacgg tccagtctgg cggcacggcg
    28981 atgaactcgg tctgcatccg gcggccggct gaattttccg gataaagcag gctgcgctcg
    29041 agcgcgtcgc gctccaatgg cggcagtttt tcaaggatct cttcctggtc ctcttcgtcg
    29101 aggctctcaa gcaattcgac ggcgtcgtcg gattccagtt cgcgtacccc ctccgcgacc
    29161 gtttcaggtt cgagttcctc gaggatttcc tcgcggacgg agtcgtcgac ttcattgagc
    29221 gccgagaagt cgaaatccgc accggtcagt tccacgaggc tgacgcggtc ctcgggttcg
    29281 agcgccgcga tcagatcgcc gagatcggcc tcgtggagtt ccgcgacgat ggcgcgcaac
    29341 aaggccgcgt cggcaacatg tatcgcgcgc gcgatgtctt tgacgaactc gctgcgaatt
    29401 tcgccatttt cgtcgcgcat tgggagacgc accggcggcg catcggcgga atgggcaacg
    29461 tccacatcat cggccatgcc gcgcctcgcc ggtttgacag aattgcacaa ctcgtctgag
    29521 ttgtcggatc acgcattagc caatgggcaa tatcaagcgc aatgacaaat atcgaccggc
    29581 taaaacgggc tcggaagttg gtgaacgtga caggggctgc cgtggctgcc ttgatcgcgt
    29641 ggacaacggc ggcgcaggcg gcggactgcc cgcgccaagg cacgctcggc acctcgcgca
    29701 ttctcagcgt cgatgccgcg accactccgc gggtaggact gaaagagtat ccgcagacgc
    29761 tgccgctgga ggatcacgag gtggtgttga cgttcgatga cggcccgtgg cccccgacca
    29821 cgccgcgggt gctggcagcg ctggcccgac agtgcgtacg cgcgaccttc ttcctgatcg
    29881 ggaagccggc gtcagaacac ccggacctgg tgcggaggat cgcggccgag ggccacacta
    29941 tcggccatca tacctggctg catcgcagcc tgatgcaaat caagccgagc gaaacaacag
    30001 aggaaatcga ccacggcata tcggcggtcg agctggcgct gcacggcgtc gcgaccagca
    30061 cgccatccac gccgttcttt cgttttccgg gctttgagac gaccgcggcg acacttgacc
    30121 tgctgcaatc gcgcggcatc gtcgtgttcg gaaccgacct ttgggccagc gactgggacc
    30181 cgatgacgcc gaagcatgaa ttgaagctga tcatcgacag actcaacatc gcccgcaagg
    30241 gcatcatcct gtttcacgac cccaaggcgc aaaccgcggc catgctgccg gatttcctgc
    30301 gctatctgaa ggataatcac tatcgcgtgg tccatctggt gccgaccgga cccggcgtcg
    30361 actttgacgg cgccccctga gaccgcacgc ggaagtcgat cggccgcggc accccgaaac
    30421 gcggctcacg cggtgagaaa acgctcgagg tcggtccgca gcgccagcgc cgcgtcttcg
    30481 tcgattccag gttcaatagg cccgccttcg gttcgcaatt cgatcatgcc ggtcaacggc
    30541 cctttcgatt tggtcgtgat ggcgctcgcc aatatcggta tcttcgattg aggggctgtg
    30601 ggcagtaagg agactgttgc tcgccggcaa caagccactg cagatcaagg catgcgcacg
    30661 ttccaacggc ttttggcata cgggcgagtt cgcggtcctg tatgccgccc aattcggcga
    30721 agcgccgtcg atgactctgg ctcgcgcgcg gcagcagctg cgatgaaaag tggcgcgaca
    30781 gaccgcattc gccttggatt gggcccaaag gcgcatgaca atgctctgga ggctacacgc
    30841 agttcacgcc gcgttcatgg ggccgcgtgt aattgtcagc acaactggga aatgcatgac
    30901 ggtgttttat ggtcagtaaa gtgttggtgg gcgttcggac cggacaatgg gccgccttgt
    30961 gcctgggact cctgacctgc agcatcgcgc caattgcctc ggccgccgat tgtcccggcc
    31021 atcccgatgc gctcggcacc tcgcgaacgc ttgtggttga tccgaaggcg catccccgca
    31081 tcgggacgat gcaatatgcc gagaccctgc cgctcaggga tcacgaggtg gtgctgacct
    31141 tcgatgacgg cccgctgccg cataacagca atcagatcct ggaaattctc gcctcgcaat
    31201 gcgtcaaggc gacgttcttc accgtcggca gcatggccca gaattctccc gagggcgtcc
    31261 gcaagctccg cgaggccggg cacaccgtcg ccacccacac ccaaactcat ccgatcggct
    31321 tcgacaagtt ctcgcccgag cgcgccaggc aggagatcga cgaggggatc gcgtcggtga
    31381 cggcagccct cggagagggc ggcgggcttg ctccgttctt ccggccaccc ggcttgcggc
    31441 gctccccggc cattgaggat tatctggcat cactgggcat ccagatctgg agcgccgatt
    31501 ttccggctga cgactggcgg catattcccg cgtcgcgcgt ctatgatctc gcgatgaaac
    31561 gcctcgaagc caagggaaag ggcattttgc tgttgcacga catccaggcg cgaacggtcg
    31621 cggcgttgcc gagtatcctg catgagatga aggcgcgcgg ttatcacatc gtccacgtgg
    31681 tgcctgcaac gtcgcaacaa ccgaacacac caaccgaacc actgcaatgg cagttgcatc
    31741 caccgtcgga gacggtggcg atttcgcact ggccaacaat tcccaatttc gtttatgccg
    31801 aagccggcac cctccccgcc ccttcgttgc cgggttcggg ttttaatgac ggacggccgg
    31861 tatcgctggc ggagccgttc gatcgcacca gtcggcgtgc gcatggcgtt ctcctgccgc
    31921 cgcaagcgcc ctggccgcgg caatcgctac ccccgctggg gaacgccgcg aacgtcctgc
    31981 cggtgccggc gcagaacatt ttcgagatcc aggaaaagcc gggcgcgcca gtgcagaccg
    32041 tcgctccatc ctcacgtcat gcggaacaag tggtcccgcg tgcagcagac gcccgcgcaa
    32101 gcttgatttc ggtcggcgcg agcgttcccc ggcgcgcaat ccccggaggg ccctcttcag
    32161 gacgcgcccg gcgggcgggt gtttcccaaa tcgccactca ccgaacccag cttgccgccg
    32221 gcgccggccg gggcgtgctg aggcattgaa tccacggcaa agctgcgtcg cggcggctta
    32281 atccgtcacg atcttggaaa cgcacagcaa gacgaccaaa gcgagaaaca gcagatcgat
    32341 gtaggatttc atttcgccgt cccggcggag aaccccgacg tcattgacga tctgcttgcg
    32401 tgaggcggcc gcggcaggac catccccggt gaggcccgcg agacggcgcg cctcaagctc
    32461 gagccgctcg atgcgctgga agaaataaaa cgaacgcagc gccgtcgccg cacgcatcac
    32521 ggcgtaggcc agcatcagaa tcgcgaggac cgcgcgttgc tgatacttct ccatcgagtt
    32581 cagactgtaa tagaccagcg ccaaaaacac gaaattggag agaaaccgat acgcgtagcc
    32641 gaggaatttc atgtatctgt tccgatcgat gatcagccgg atagtcttgg tcggacgcga
    32701 tccgaagtat tccggtcggc agggtggtat caccagcctg agcctcgcgt ccatcgatga
    32761 acgccaacgt tgttacagca aagcaaacgg atgcgccgtt cggcatcgac cgggttccgg
    32821 ccggccgggg tttcggtatt ctgcggccgc tcgagcatca tgcattcaga tccaggccgc
    32881 ccgccggcgc ggccgccttg caagactgcc tggagcgcgt caacccccac cacaggcttg
    32941 atttcacttt gtttttttga attcacggaa cctctgtatc ccggatctta ccccccttgt
    33001 tcgtcggcca gccggaaatc taccgccgag gctacctgcc agccgttgcg atcgacgctg
    33061 atctgcaagg agatggggca gcctctcctg ccacccgatt cgttgccgtc ccccgacgtc
    33121 acaacacgcc aacaatcgct gaaccaaaaa gcgtcccagc cagggcggtg gctgagacgc
    33181 ttcggaggtt tcgccggatg agctgctccg gcgatggtgg caacgctccc tccgataaca
    33241 agactaacgc ttggctggcg atattcctca atcctcgcgc gttttgaatt cttcaaaaag
    33301 tcttgtgacc gtccctcgcc gtgatgacag caccgtgagg gaagcgacca aatggcctca
    33361 agcagccggg ccgtcgttct gcattttgaa tctgtatcgg gtgtacggtc ctcgcacagt
    33421 ctattgatcc atcgtcgctt ctgttcgtgc atgacgcgtt ccggcctcaa accgtcaaaa
    33481 cttcagctgc actccgcgcc aatgcgcacg acaagtgccc atcgcgatgg ttggacaact
    33541 ctccatatgg cggcgggaat atcaggactc ccatgagggt ctggctgtca gaagcgctca
    33601 tgcgcagcag gaactacatg gagtcactcg atgactacac ttacgcttgt tggcgcggcc
    33661 acgattcttt cgacgacgct cgcaactccg gtactcgcgc aagctcttat ccaggagccc
    33721 ggcgtttacg ccatctaccg tcccaacgga gatctaggaa ttggagctac gccacctcag
    33781 cgaccaggcg tagttgttgt tggtcgcgga gccgccgacg ctgtggcacc agcaccgtcc
    33841 ttccgtccgt cgacaggcgg aagagaaacg acaaccagac cctggtcagc gccggtcggt
    33901 caccgccagc cgcgtactgc cgatgttccc acatcgacct ctggtcggat tgcctgccga
    33961 aaccgctgga cgtcaagaag ccgagcgcaa tgtgattcct tttgaacaca gcgccccgag
    34021 gcaatttgcc tggagcgtcg ggcattgcct gagcgtgcag gcaagtactc ccaaaggaag
    34081 actctggttt cagtgatgtt attcccgcgg cttcgacggc cttacggacc cgcaagattc
    34141 ctcgccgccg ctctcaggga gcgtgacgcc ggcgctcagc cgctttgacc agggaagcct
    34201 tgccatcaaa ccaggatttg ccgccgtcgt tgcgacccga attcacaacc attctccgaa
    34261 agtaggcggc gggaataatc ggtgcgctct tccggtcttc cccttacgcg gcggactgga
    34321 tcgcctcacg acaaaacgac gctacgtagc cgaaaccaag gaacatcatg gccaaacagc
    34381 tacactatgg aacgaccgcg aaggtatttc actggctgat cgtggcgctg ctcagcgtgc
    34441 aatacccgct cggctggctc atgccagaga ttcatcgtgg ggcgaagccc agtgacgcgc
    34501 tgacgttcca tgtttccctt ggtatcacaa ttcttgcgct catcgttttg cgtcttctct
    34561 ggcgcatcac ccatcccgtc gcgccggaac gttcgcttcc ggcctggcag cagctgatat
    34621 ccgaggcgat gcactggctc ctttatgcac tggtgcttgc gaccacgatg accggctggc
    34681 tcttggcgtc cgagcatggc tggtcgatct ccctgttctt tgcggtctct ttaccgatgc
    34741 tgacttcgaa aggctcgctc ctcgcgagtt cgatcgggaa atggcacggg accatggaat
    34801 gggctttgct ctctctggtc ggcgcccatg tggcggccgc gatggcgcac acgttcatct
    34861 ttcgcgaccg gatcatgcag cggatgctgt cgacgaaccc catgatcttc gataccaaca
    34921 gagaaggccg gaaggcctgg gtgaagaccg cccgggatcg tggggtgaac tgagggggtt
    34981 tcggagggct aacgcgggtg attcttgtcc tcgttttgtg tgaacaacgc gtattcggct
    35041 atgggcagcc gatggctctc ttcattgatc catggcttct ttcggaaagc cggaaagcac
    35101 agcctcctgc gcacggccga caaggccgcg cacgagaaac gcagcacgcg gagtgtcccg
    35161 cgccttgaat gcctgtatga tgtcatcgta tccgtgcgca gcgcgctcgg caaactcccg
    35221 gctcacgctc gtgatcgtgc cgcagagctg gatttgcagc gcacaccgcg ccagcgcatc
    35281 gatgagcgcg gcatttccgg attgccgggc gatagcttcg taaaattccc gatcgcgcat
    35341 ggcatagatg atcatgtctc cgcgccgcaa ggcggccacg gcttcatcaa gcatattttc
    35401 ataccgtccg atgtcgcggt cgctcatgtg cggtaccgcg agttcgaccg caaaacattg
    35461 aagctgcttg tatagctcgt ataggtccgc ggtttcgttg cgagaaaagc tgcggacata
    35521 aaagccgcga tagggcttga tctcgatcaa ccctcccttg gcaagtgtcc cgatcgcctc
    35581 gcggaacggg gtccgactca cccgcaacct gttgatcacc agcttctcgt caacccatcc
    35641 gccactcggc aattcgccgg agacgatcag gcgcacgact tcttcataaa tccgctcgcg
    35701 cagggttttc ttatccaatt ttgcaagact tttgatgacc atagacggtg cgtctaccag
    35761 acagcccagc tgggtttacg ggtcaggacg tcccagcgga ggaatagacg atctcgctcg
    35821 tctatttgac ttccggcgcc ggcgatcctg cggaaaatgc gcgctcgaat gtctcaagat
    35881 ctgcgctggg caggtccgaa acggcaatgt acgtgaactg cgcatcgctc cagctccgga
    35941 cgttgtatcc tgcaatcgcc tgatcagaga cagatcgacc gggcggcagc gtcgtcaggc
    36001 tgacggtatg cgcggcatgc ttgtagacga tcgttgcaac tggatcgtgg ccaacgacat
    36061 ccacgcggcc gcccaggagc acgaagccct gcgccgcgag gtcaaccacc tgaggcgatt
    36121 ccggcaggcg tgatgtgaac cagggcttga ccgtgtggcg gttggaagac gcaacgtcga
    36181 aaggctgagg ggccaattga ccccggatat ggcctgcaac gacctgacga gcaacgtcct
    36241 cgtgtagatc ataacgcgct atcgtcagca tgacagaact cgagatcact gccccgaaca
    36301 ccgctgccgc ggccaacgcc tgaaacgacc agcttcgctg acgtgtacct cccgcctgac
    36361 gcatacgtcc cgcctgaccc ggacctcccg cctggcccgg acgatccgca tccactgtcg
    36421 atttgatacg ggcccgtaga cctggaggca catcatattg cggaatcgaa cgaacggcgc
    36481 ggcgcaacga cagcaaccgg tcatattgag ccttcaaaga ttcatcgtca gccatgcgcc
    36541 gctcgaactc aatcgccgaa accggatcga gctctccgtc gcaataggca ttcaaccgta
    36601 gttcgtcact atctttcatg gatgcaatca taacttacct tcggtcacct agaagagcga
    36661 tgagatattg cctggcccgg gacaaccgag acatcacggt gccgatcgga acattggtga
    36721 tctccgcgat atcgcgatag ttcaactggt ttatttcccg caaaacaatc acctcgcgat
    36781 attgcgccgg cagctgcgcc aacgcctgct gaacgtcttc tgcatccgcc ttggaaaggg
    36841 agatctcctc gggtgtctct atcctcgttc cctgcgggct ttcatgctca agctctcgtt
    36901 gctccgtaac actcaggtcg tcggtgaaaa cgacggactt tggtctattc ttcatcaacc
    36961 agctgtaggc cgtgttgcgc acaattgtca gggaccacgc gcgagcattg acggcaccga
    37021 acttcctgat gccgcgaaat gcacgcaacg ccgcatcctg cacaacgtct tcggcgtcgt
    37081 ttgcattgcc ggtgagccat tgtgccagac gataggcctc ggccatgtgc ggcagaaaga
    37141 cttcatcgaa cactgcgcga tcatccttgt tctgcacaat tgagcccgac gcttgctgct
    37201 ggccagacac acgatctgag ccgtccgacc gttccttcga cacgacttcg ttaagctcgg
    37261 tacgcctgaa gttcgaccgg cgtaccgatg agtgtgtcgt catattcgcc acgcttaggt
    37321 tctcaaggct ttggcgttac ttcgcgatga tttgtccgac catgtgggga tgcagaccac
    37381 agaaataatc gaaggtgcct gcactggtga acgtatagga atacgattcg tcagtatcga
    37441 gcaccttgga ccgaaacgtc gtctgcttct cgaccaccgt gtgcggaatg tcgtcgtgat
    37501 tgacccactt gatagtggtc cccacagcaa ccgtgagtgc cttgggcgag aacgtgaaat
    37561 tgtcgatcgt gatcacgttg gcggcatcct gcgctgctat cgcaccaaac gccagcaacg
    37621 ctccgatgac tgggcccaaa agcaatgcaa cgaccagggc gcggtcaaga gcaaaccggg
    37681 ccgcagcaag gcggagttgg ctgatcggaa tttgcatgca ttatcctcga tcaaccggcc
    37741 aacgtcttat cgatgatcgc gagcggcttg ttaccttgcg cgacagtgac ggtggacaac
    37801 cccagcaagg tgcgaagttt gtccgcgggc accagcatcg ggcctggcga aggtgcgctg
    37861 ccgggagccg gctgaggaaa agccgtcgat agggccgtgt ggaaggtcat gttgccctcg
    37921 accttctggg ccaactgatg aatgtgtccg ttcagaacgg tgaccgaacc gaagcgcttg
    37981 agcagagcaa gcgcctgcgt accatcgtcg gtaccccagc cccattcctt gtagatcacc
    38041 tgcagcggga tatgtgcaaa cacgacgatc ggcgtgctgg ccgacttgtc cttcaggtcg
    38101 gcctcgagcc atgcgagttg ctcggcgccg agattgccga gtccgccgcc tttcagatcg
    38161 acgacgttga cgagaccgat gaagtgaatg ccatggtcgt cgtaactgta ccagcccgcg
    38221 cccttcgaac cctttccgta gcgttccaga taggctttgc cgcgctcttc atcgatgatg
    38281 tcgtgctcgc caggcacata atgaatctgg acgccagtct cgccaaacac cttgtcggca
    38341 ttatcgaatt cggcaggctt ggaaagatgc gtaatgtcgc cggtgtgaat gatgaaggcc
    38401 ggcttgaccg gcatcgcctt taccttggcg acggcttcgc gcagcgtacc gagcgcgtcg
    38461 gggttcgccg ccttgtcaaa gccgatgtga ctgtcgctca tctgcagaaa cgtgaatccg
    38521 gtgggctctg cggcgagcgc gtcgccgatc aaccccactg agtggggaac gccaccgctc
    38581 acggtccaga gcaccccggt tcccgcccag accatgcatt ccagcacttc gcggcggcct
    38641 aatccccgcc catgatcgtc aatgcccttc ttagccatgt cacatctccc tgtggggcca
    38701 agcggctccc aatggggccg ctgaggagga gactcggccg tctcgagctt tattcccaaa
    38761 taatatacaa ttcccgggat ccgtggattg cccccgctag aaccgcgcag gactttcttt
    38821 tcaaagagac gccgctcgca agctttccgc ggaggccagc agcgaacaca gcttcgacat
    38881 gcgcacctgg cggcagcggg accgtcagct ggctcccggc ttgacgatga ctttgccttt
    38941 catgttcggg tggatcaggc acaagtagtc gtattcgccc gccgttgtaa aagtgaaaga
    39001 gaacttgtcg tccttgacga ggatcttcga tcgaaaccct ttgtttagat cggccacatt
    39061 gtggggcatg gcttcattgt tgacccaggt cacggtggtg ccaggcgcga cggtgatttc
    39121 gccgggaatg aaagtggtat ggtccatgct cacggtcgcc accgggccag cgccggcatt
    39181 attcgaatcc tccgccaggc aaggagtgga cgccaaagcg atgaacagga ccgtcaatct
    39241 caaggcgtgc agaacgcttg gcgacctgga accagagacc tcgaaggcag ttcccggatt
    39301 tgcggcgaag ttcgtggagt cacggactgt caaaacgaaa tctccttggt tcagttgaag
    39361 ttgtctggcc gaggcgcatc gatcatcgtg ctcgccggcg tagagaccat tcagaatggt
    39421 agtagcgtag ctgcggctcc tgtgaatttt ggcgtgagcg cgcactgccc cacagaagac
    39481 tcgacttcgc gtgaagaaat tcccgcactc ttgcaaatct ttctgaatta ttgaggcctg
    39541 ccgtccggtc cgttcgacgc atgcagcaaa caacggctgc gatcaaaaaa gagtctcagc
    39601 cgggcatgac tgagacactt cctggaagtt aggccagttg aacggcggtt atagggctca
    39661 tcgatctctt ggtaggagac gcctcctttg cttctgatgg agagactcag acgtccttgc
    39721 cgatattccc agggcggtca ctttggattt ccgttgtaag cggagtgaac aaaaaagacc
    39781 ccagctttgg gagaaaagct gaggccaggt gcagcgaaga ttaccgagac tcggattcat
    39841 gtttcggttc gaaggtacga ttagacactg ccttttgtgg cgtgctcgct ctcatcgaca
    39901 tgacgatttg cgtgacacca cagcagagac ctatcgggcg cgcgctttat tcccacgcct
    39961 tttggtcgat cacgcggaac gtgtcgtatc ggtcacgcac gaaagcaggc accttccgca
    40021 accacgtacg ccggtgatta tgggtcccgg ccggagcctg agcctccagc gagaccgact
    40081 acaccggccg catcagctac cagcgtgtgc cccccgcctc tgcgaaagtt gcctagcgat
    40141 cccatttatc attcaagatt atcggcctct ggacgaagcc cgcggcttcc ttgttccgtc
    40201 catgcttctc gcgccatttc ggccccggac ctcgcatgta atgggcttcg ggtcggtatt
    40261 gcctgaccga acctccccaa aacgcgttca ggagatccag aacggccgtg ctcaatcgcg
    40321 cccgcatcga gtcgagagaa agaccgccag aagaatgcaa actcaccaga gcctcctgtc
    40381 tcttcggcaa tggttatcag cacggccccg gtcgtgatcg cagaaactct tgccagatcg
    40441 ccgagcttta caatcgaagc aagatgacga tgctatttcg catccatttg ggatgatcgg
    40501 cgcgacgacc gatttattcc cgccaactca atctcccgcg gcctgacgta gatcgcgagg
    40561 tggtgcctcg cgagggcaag ccgcggtaac atgaaagagg cggcctgatc ggccgcccat
    40621 tcaacccact atcccgcgag gtcatgaagc gctggcagac acctcattgc cagacgccgg
    40681 cagcaatgct caatcgatct cgcatcagaa ctggaagcgc atgccaacgg tcggatcaac
    40741 cgagtatgtc tgcaggaagc catttgagag accattcgac acttgcgacc acatgatgcc
    40801 ggcataagca tcgaagcgct tcgcaaactt gtaatcggcg acgaaggaaa ccgcatccag
    40861 ctgtccactg cattgtgcca ggctggcgtt cgaacaatgc gccgcgttca aggtggcggg
    40921 tgttcctccc tgaaagctgt tctgctcttc gtgataatag gcgacactta gatcgacgtc
    40981 gggcctgacg gcatatctga cgccacccca aaatacctgg aggaccttgt cggtggcgaa
    41041 gttggcatta ttgaccgtgc caagcgtata gccgcccacg atggactctc ccgtaagcaa
    41101 ggggtttttt gggttggcga attgcatgtg ctcgtaaccg ccgagaattt tcaccggccc
    41161 gatggtgtat ttggcgagca gcatgaagct ggtattgtca gagacagttc ccgcaacgaa
    41221 tccatttccg ggatttatga gtgttggcga cagcaacgtg cccaccggca gcggcgccga
    41281 cgtgatggca tcctcaacgt gactgaagat cgcatcgacc gacagtccca tatagtccgc
    41341 accgatttgg ccctgaaagg cattgcctgt ggcgccgccg aagccggctc gaagctgcgc
    41401 ttccgcggcg gcacggaacg gacctacatt cactgcatat ttgaaggaat tgtcgaagat
    41461 gcgattctcg gtagctccgc caccgccggt cgccccctgg aaaccgatca aagagaacgc
    41521 gcccgaattg cccatcgggt cgtagttgac cacgccatcc gaggtgagcg cgctttgccg
    41581 tccgtaagtc agcgttccat aaatcggcga gctaagaccg gcgtaggcgg ccacattgaa
    41641 cgcctggccg tctttggcgg aatcggcaaa cgagttctgc gcgccgaccg ccaacccgtt
    41701 gttttggaca atggacccta gtccgtcgga aagtctccct gaccatgggt taaatcccgt
    41761 ttgaagattg aatacccctg atagtccctc ggtcagctcc acatttccct taaggccgac
    41821 aaatgacgag ctcagcgcgt tgccgccaat gccgaaataa gacccgttgc tgttctttga
    41881 gatcagatag ttcaatccaa gcccggaggt gctgctcagc ggggcagcgt gattttggta
    41941 ggttatgccc aaatcgacca agccatacag cgtaacgcca ttccaggtca ggctgtcgtt
    42001 taccggcgga gctttcttgt actccgcttt gtaactggga tagtcggcgg ccatcgaatc
    42061 cgccggttgt tttgcaacag gttgcgcttc aagcttgcgc tgccgccgct caagatccga
    42121 gatacgtttc gtcaacgcct gattctgttg acggagctgt gtggactgct cctggatatc
    42181 agatagttct ccggcatgtg ctgctgttgc gataccgaaa acagccgctg cagatataaa
    42241 accctttttc acaagcgtcg cctcccgacg cggccacacc agccgcgagt ccaccacgat
    42301 cacggagcat cggatgatga tcccctgaca gcgagactcg tgttcgcgtg cggaaattcc
    42361 cattgggtaa atattttttt tcatctcctg catcaattca aaaaccgtca cgtcggcctt
    42421 gttgcctgcc ggtaacagaa tttcgtcgcc ccacttgcta gggagccaat gcgcacgcgt
    42481 tgttcaaaat gaaattccgc gaatgcgtgt cgaaatcgac agcatcaaaa aaacaaacag
    42541 atgaggaaac agatgagatc cagtaaaatg ctaattgcgt tagccggcgt cgcggcgatg
    42601 gcctggacgc cagcgcatgc atatgaggcc ggagcggccg gctgggccca aaaacccggc
    42661 attactctcg gcggcgcgag cgcgggcgtt ccgccgcccg gactttacat gatcgatcag
    42721 gcgttcacgt atcagtcgaa tcttgcaggg ccaggcaata gcgtcatcaa cccgcacggc
    42781 acgacaaccg gcgtgccggc tgcagttggg gtaaccgcct tcgtctgggt tcctggctgg
    42841 acctttctcg gcgcaacata cgacgcggcc tgggcgatac cggtaggcat gtttgcagcc
    42901 ggcagtcctg tgaatgtcga ggcgtcgggc atgatcaaca cttttatcgc gcccgtagag
    42961 ttgagctgga agcttgggga cagcgggttt caggtgaaga cgggttttgg gatgtacgta
    43021 ccggacggca ccgtcaccgg cgtcaacggc cttggaaacg tcggcaatgc gtggtggagc
    43081 ttcatgcccg aattcgtcgt ttcataccta aaggatggct ggaacctcac ggcgaacgtc
    43141 ttcgaagaga tcaacaccaa gagcacggtc acccagtatc gcagcggtga catcctgcac
    43201 gcggagttca ccgcgaccaa gaccatcggc aaatggacgg tgggacccgt ggcttactat
    43261 gcgggacaag ttagtgacga caagtccagt gcattttatg gcggggccat caatgtcaac
    43321 agatacaata tctgggcggt gggcggctcg ctgggttaca actttggacc cgcgcagctg
    43381 accgtttggg cgctggatga agtttccgca aacgcttccg gcggcacacc gcagcttccg
    43441 cccggacccc tcatcagcaa aggcttcact gcatttgcgc aactctcata tcgtctctgg
    43501 gcccccgacg agcccgtagc ggcgcccaaa tctccgatct atcacaaata gccggaagat
    43561 ttgacctaga ccattatgcg cgacacgggc ggccaccgtt cagatgcggt acacggacct
    43621 atagcacttt gcgggcgcgc cgggatttcg ctctcttgta aatccgagcg cgcgatgccg
    43681 aaacaaacag gtagcgacat ccggcttttt tgctgcgcac ccggatgtcg cagcccgcac
    43741 cgcgggaacc aacgcaagtg gcaatcacgt cacgccaagc ggaggcgccg cttcagcgcg
    43801 aagaggcacg tgaatccaga tagcatgggc tatacatggc cgcgagctgg cgaccgctca
    43861 ggaaaatcat gtgtggcgtc agccctccgc gctggtcgat ccagcgccgg acctcaaatg
    43921 gatacatgga atacagcgtc tgcgttgctt cgagattggc tattctgcga ccgttggaac
    43981 cgggatccca ggcggcgtga aatccgagct tggcgcggga ggtcacacag atcttgtcat
    44041 gggcaaccgc accgagaacg atggtgcagg ccgaggcaca aaagccgtcg atgatcacca
    44101 tttcgcccga gccgcgcacg ccgtgatatt tatcgacgta ggtctcgatc cggccgccgc
    44161 gatcttccac aatccgaacc accgcatcgc ttgcttgaac ccctgcgagc agcagagccg
    44221 ccgccagtaa cgctgtcaga cacctgatgg ccattgccaa ttccaaactg ccggtctcga
    44281 atctcacgca tcaagcgatg ctcgctggac atccataaag cccctggacc aagccggtat
    44341 ttgggccaaa ttccgccggg ggaatgtata ttgtttaggg caataatata ttgcgccaga
    44401 ctgactttca tccggttcat cccgccccac cgaacaagga tcgcagggca gccggatcgg
    44461 catcgaaggc tatcaggtgc gcaaacgcac cgacggccgg aacgcgactt cacgatttcg
    44521 agtgccgggg aatttcggaa atccgatgcg agtctttcca tcagaggccc acgcaccatg
    44581 cgcactgggc caacaacgtg gaaggatacg acgatgacca atttcaagct tcttagcgtg
    44641 ggtgtgattc tgtcgacggc gatcgtaacc ccggtatttg cgcagcaagc tgtctccgaa
    44701 cccggcatgc aggcgtttta ccagagcctg ggtgttggat cccagtcaag cgcgccgagc
    44761 gccatggcgt cgacacgcaa cgtctcatat gcgagcacgc cagccaaacg catttcagcg
    44821 aagcacaaga tgtaacatgc cgcaccgata agtcggtgcc caatcggtag caaaggcggt
    44881 cccagcgggc cgccttttct attagccgac gccgtgacgt tttattgcct tccttggccg
    44941 gccaggttga cgcgcgattt tgttgcgaaa accatctcac taagtttgtt gcgaaaacca
    45001 tctcactaag ctattttgcg gcaagtatat acaacttcac gtttttgccg acccatttta
    45061 atcgactccg cgagatactc gctccgattt agtgctggcc aaaaaaatgc cgctcgattc
    45121 agataccaag ctaccgccct cagctgaaat tgagaaaacg ttgctatgcg aggcgcgtat
    45181 agaaaagctg attaatcttg ctttcaaacg ggaagttatt cgcaaagtta cagatgttat
    45241 cttgccgctt aacgacggcg ggtgtggtcc agcattctat tgcgtccacg cgatcggtgg
    45301 tgttgccgcg gactttcgac atatggcgag gatgcttggg ccagaacaaa aattctatgg
    45361 cattcaaaca ccgaccgaca aacgcaatgc tgaatttgtc agttcgattg gatcgattgg
    45421 ccaatactac gtggacgagt tggtaaaatt ccagcccgag gggccctttt ttttgggtgg
    45481 tcactcagta ggtgcgacgg tcgcgctgga aatggcccag caattgatag caagaggccg
    45541 ggaagtccgc ctgctcgtgg ttttcgatgg atggctctta aataccggtg ccgatatttc
    45601 gtttctaaac ccactctatt ggttcaacct tttatgttac ctgccgcgat ggatcaatga
    45661 agtgctgttg gtcaaatact cctttcaagg tgtttgtcgg acgctcgtga aaaaattcag
    45721 ttcgcgccac ggagtagagg gtgttgtaaa tctaagcgcc tttacacccg agcatgcggc
    45781 tttcatgaga ggactttatg acagacatct tgaatacgta ccaaatagtt atcctggtcg
    45841 cgttctggtg tttgtcgcca agaccgatga actcctgaat cacggtcacg ttaaagcggc
    45901 ttggaggaaa atcgcaccgt cgtccgaaat cttcgaagtc attggcacgc acgtcgatat
    45961 aatgcagatg ccacgcggac tccctgtcgc caagcgatta agggagagaa tcaaacaact
    46021 cagcgaacgc gcatgacgta aggcatgcca ttcaggaata gccggttgtc gtgcggcgcg
    46081 ggctgccgta ctcatctgcc accgataata cgttacttcc gtcacgaata cgcttggctc
    46141 aggccgtgtt tccggcatcg atggtcagca cgctgccggt gatgttgcgg ccgccgtcac
    46201 ccaagagata ttcgaccatg cgcgcgatat cgtcgacctc cggcagccgg cgcaatgcgc
    46261 ttcggccggc gatccgcttg cgctgatctt ccgagagggt ttgcgttagt tcagtatcga
    46321 tgaagcccgg cgcgatcgcg ttcacggtga tcccgagctt gcccacctcg cgcgccagcg
    46381 atcgcgtaag gccggtcgcg gccgccttgg tcgcgccgta gaccgacagg ccgttatatc
    46441 ccgtggtcgc gataaccgaa gacatgttga tgatgcggcc ggcgccatcg gccatcatgt
    46501 gccgcacgac atatttcgtg agaatgaccg gcgacaggat attgagacgc accagggcct
    46561 cgatctcggt gttgtgcatg gtggcgagca ggccctcggt gccgatcccg gcattgttga
    46621 cgaggccata gatcgcgccg aattcgtcgc gcaatccttt gacaaaagcc gggatggcgt
    46681 cggtctcgct gagatcgaag gccctgaagt gaaggccgcc aatgccttgt tccacgactc
    46741 cgcggatggt ctcgcgcaac tcgtcgcttt cgcgccgcgc caccgcgatc acgttgtagc
    46801 cggccgccgc gatccttctg gatatggcaa gaccaatgcc gcggctcccg cctgttacca
    46861 caacattatg catcgtgccg cgccagcttg ccggccgccg taatgtcgag cgacgatacg
    46921 aatttgatca tggcgggcac cttgtgagac gccaacgatg cccggcaatc ggccagaatt
    46981 ttattccgga catcatcgct ccggctcacg tcgcagccat cggccagaat gacatcggcg
    47041 accacgatgg agcccgtgat cgggctgcgg cgcgatctgg cgcgggacat ccgcacttcc
    47101 gcgtgccggt tgatgacggt ttcgatttcc tcgggatgaa ccttgaggcc gccaatattg
    47161 atgacgccgc cgcgccgtcc gacgaagtaa tacctgtcgc ctcgcagctc gatcatatcg
    47221 ccggtgtcga cgaacccgtc gccgtcggtg agcgcggtcg cgttccggcc gacataggca
    47281 tgcgccgtcc gcgtcgaacg gattcgcagc gatccgtctt cgactttcat ctcgacacca
    47341 tcacggttca ccccaaccat agttgcagga aagccctcga ggccatcgtt cacggcaaac
    47401 ccgacgcctg cctcggtcga cgcataggca tgaccgatcg aagctgccgg aaacgcccgg
    47461 ctcagaccat cgagcacggc ctggtctgca atttcacccg acaagcggac gtagcgcgga
    47521 gaaaagtctg aagccgatcc actcatcaga agcttgcgcc aatgcgaagg cgtgccggat
    47581 atatgcgtga cctccctcgc cttcaggcgc gcgacatgat cggcgatcgg ctcgcccggc
    47641 tccgtcagca ccatcgaacc gccgccgatg acggcgcgca agaagatctg aaggccgcca
    47701 tagcgcctga tgtcatagaa ggtcgcccac accggcgcgg cgccgcgggc cggaccgtct
    47761 gcggcaatcg ctccggtcaa tccctccaga gtatggcgca cgattttcgg cacacccgag
    47821 gtgccggatg tcagcatcag ccaatctgtc gcgtgctcgg ttgttgactt tgcggccgaa
    47881 cgcgccggcg ttctgtccgc aaccaccaga tccactccgg cgtccgccca gcgtgccggc
    47941 tggtcggtga ccaccgcgtc gatttcagca tcctcgatca ggtcattgac gtgatcggca
    48001 ttgagatccg gcggacatag cagcatgcac cgcgccacgc catcgatctc cgtcattgcg
    48061 atggccgata tcagctgatc tgccgcggca agtaacaccg aacgcccgga gagcgcgcca
    48121 agacggcctg ccaggcaggt ttgttcgagg atattcgtca gggaccaaac ctgtcgcgca
    48181 tccgaaatcg tgcggccctt cagctctgga ctgagatagt cgcggagcgc gaagatttca
    48241 cgcgaggaca ttttcgtagg ccttgatgaa gtcgcctacc gtgagaggaa attctgcgtc
    48301 ctccgatatg gtaaacgggt cgatacccag atcgtcttca agccgagcca ccaggatagc
    48361 aaaggcaagc gaatcgaaac ccgtctcatg aagccgcaca tcatcattga gtggcggcag
    48421 tgtgatctgc tgatccttgg caatctgctg catcaccgac acgatcctta acgctaccga
    48481 catggtttat tccgccttct ctttgaggtt ttgatagata tcacagccgc agcgagccat
    48541 tcgaaataca cttataactg tctgcatcga tcggacgata ccctccagac ttcgcgtcca
    48601 gataaaacag cggatccgtc acgaggcggg aatcgaatcc ctcgcgaacc aactgttgct
    48661 tcttttgctt gaaggtgttg gtggtatcca gcgcggtgca aaagcggacc atgacggggc
    48721 atgcataggc cggcaaccgg cgccacagac ggttccggaa ttcggcgagg tcgaaagtat
    48781 cgtcgaccac gaccgcagcc attccggccc ggccgtcggc gccgggaatc gccacgccgt
    48841 agatcgcagc ttcgacaacg cccgggcaat ccgcaacagc ctcgtgcact tcggaggtcg
    48901 cgacattctc gcctttccag cggaaggtat cgccgacccg atcgacaaag tggaaaaatc
    48961 cggcctcatc gagccgcaac agatcgccgg tgcgaaacca ggcatcgcct ttggccaaca
    49021 catcgcgcag gatcttcttt tcggtctcgc cggcatcggt atagccctcg aaacggcctc
    49081 cgccgtcatc tccggttccg atccggccga tggcttcgcc gacttcgccg cgggcgcagg
    49141 gaatgcaaag accgtcttcg gtgcgaacgg gcgtgccggc gtccatatcg acccgaacaa
    49201 tcgccgccgg aaagcgatgc gccaacagcg gcggaatccg gccgatcgcg cccggcttgc
    49261 cctcgacatt gtacagcgag aaattgccct cggtcgccgc gtagaactcg agtattcggg
    49321 gaatcgcaaa gcgcgcctga aaggcctccc agatatctcc gcgcagacca ttgccgcaag
    49381 cgagccgaag ccgatgttga ctttcgtatt cggaaggagg cgctttcaga agataacggc
    49441 agagttcgcc gatatactgg aacagcgtgc aatcccggcg cacgatatct tgccagaagg
    49501 tgctcgcgga gaatttgtcc gacagtacga cggatgctcc cgcgttcagc atgctgcatg
    49561 gcgcgacgat gccgccgacg ctgtgatgga ccggcaggca attatacagc cggtcttcgg
    49621 gggaagcgtc ggtcagcccg gcgaaccagc cgccccagtt caggatgcgg cggtggctga
    49681 tgctggcggc cttcggcggg ccggtggtgc ccgaggtata aatcaacagc gcacgatcgt
    49741 tgatggtgac cccgcgccgc tcggcaggcg acagcgggct tccatccgtt cgatccagcg
    49801 cggcgtcgat actcgcttcg ttgccgcggc tgccatgggt ccagattttc ggcgcgcggt
    49861 tcaaatccgg ccgcgccgct tcgaatacat cggcaagatc gttcgcaagg atgacatggt
    49921 cggcatcggc aacattgacg cagtgggcaa gcgacagccc aaccagtctg gtattgatca
    49981 gcgccacgac gccaccgacc ctggtgatgc cgagccaggc ggcgagataa tccggccggc
    50041 tcggcatgac gaggcacacg gtatcgccgg cttcgattcc ggccgacaac gcccagcgcg
    50101 cgtaacggtt gatccgctcc gccaaggcgc gatagctgaa cgtctcggct tccgaaatca
    50161 gcgcaggacg gtccggttgc cgtttcgccc aatcctccac gaggtcggca aacaaccggc
    50221 ggggatcggc ctcgatgcgg gacgtcagtt cgatggcctt gagccagctt ttggcggggg
    50281 acggcctgcc gctcggccgc gagcggtctg tcgacgggtt gatgacacca gcgttcatgg
    50341 tctgcctgcc caggtatcca gcataacgta tagccgtgtc agacggccgt cccggctttg
    50401 agctgaccga cgatcatcag ttccaaagtt ttgaccgact ggaaattctc tggcgttatc
    50461 tcggattggg gaatggtgaa atcgaactcg gcttccacac ccagcatcag gttgaccata
    50521 tccatcgatg tcagcccggc attcacaagt cgcgattccg gatcaacttc gacggcaatc
    50581 gagttctgtt ttaggatggc cttgacgaga tcaaggatcc ggtcccgaac gttggcgtta
    50641 aaaatttgca tctccgactt tccattgtgt caatgcgcca tggcgccgaa atgagccatt
    50701 aaaatgcgta ggtatagttt tttggaagac tcttgtttaa ccaagccttg attttaactg
    50761 ccaatgcggg aatcacggga tttaaaaaaa cctagattaa ccctcgacat tgaaacaatg
    50821 gtagtttaaa tctgtttaca ttaaatcagt ataattaaat atattcaatc aaatctttgg
    50881 ccccgacgct cgatcggaag ctgttagggc gagatgcttg ctcagggggc atatcggcca
    50941 tgaacgtgca agaagccagg ctccgcaacg ctgtcgcgga cagaatcagc gaacaattcg
    51001 cgcccgaccg gagttccttc gtccggcgag cggcagccgt cgcggccgca gccgctgagg
    51061 aagccgagga cgtcgatcgc gacgcgcgtt tcccgcagaa ggccatcgac attgcgcgcg
    51121 agcaaaggct gctggggatt cagatcccgc aacaattcgg cggcgacggc gcctcgatct
    51181 tcgacattgc cgacatgtgc tacacgctcg gccgcgcctg ttcctccacg gcgatgatct
    51241 ttgcaatgca tcagaccaag gtcgcctgcc tgatccggca tggcgccggc agcggctatc
    51301 acgaagccct gatgcgtcgc gtcgcctccg aacagttgct tctcgcctcc tcgacgaccg
    51361 aaggccaaaa cggcggaaac atccgcttct ctaccgcggc cgtcgagcac gccggcaccg
    51421 agatctcact ggttcgcaac gcaaccgtga tttcctacgg cgcgcaggcc gacggcatcg
    51481 tcaccatcgc ccgccgcgcc aacgatgccg cgggctccga ccaggtgctg ctggccatta
    51541 ccaaggacga ttacacgctg gaacatagcc tgggatggga aacgctgggc atgcgcggaa
    51601 cctgcagcgc cggctttgaa ctgaaattca aaggttctgc cgaacagatc ttcccggaag
    51661 catatgacaa gattcatgcg cagacgatga cgccggttgc gcatctctgc tggtcttcga
    51721 cctgggccgg gatcgcggcc gcggccgtgg agcgcgcgca gatgttcatc cgcaaggccg
    51781 cacgcggcgc gggtggccag atgccccccg gcgctgcgca ctttaccgcg gccaggatga
    51841 cgctcgccaa gctgcgcgcc atgatcacag cgaatctcga cacctatgcg gcccgtcagc
    51901 acgatgagcg cgcgctctct tcg
//
LOCUS       DATNOI010000718         7313 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_112174,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000718 DATNOI010000000
VERSION     DATNOI010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112174"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VN968_28750"
     CDS             <1..287
                     /locus_tag="VN968_28750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33259.1"
                     /translation="GWSDNRIIKALETSASMVYRVRKQLVSADQLNNLRTFDWKRPCL
                     MESLFLGRSFRDCHIGEVMAVQVGPSKGRFGGYRENVIVQHSPAPKVVIF"
     gene            complement(1042..2256)
                     /locus_tag="VN968_28755"
     CDS             complement(1042..2256)
                     /locus_tag="VN968_28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXL33260.1"
                     /translation="MISADQGSPLKQRSFLLFLLTRSFSRFSSQVAAVAIGWQIYDLT
                     GSAFDLGMIGLVQFLPTALLVFVAGHAADRYERKRVVQICQLAQALTALFLACGAYSG
                     SLTALQIFIATGVLGTATAFESPATAALLPLIVPAGALQRATALSSGSAQIATITGPA
                     LGGLAYAVAPGMAYGMMVVFWLSGAILTGAIRLAQLAVVRDSATPADLFAGVRFVRKN
                     SAILGTISLDLFAVLLGGATALLPIYARDILQTGPWGLGILRAAPAVGALVMTAVLAR
                     HTINRRVGVRMFQAVIVFGVATVIFAVSQWMWLSVVALAILGAADIVSVVIRFSLVQL
                     ATPDEMRGRVGAVNFLFINASNQLGQFESGVTAALFGAVPAAVLGGLGTIAIALLWMK
                     LFPTLRDVERLG"
     gene            2449..3699
                     /locus_tag="VN968_28760"
     CDS             2449..3699
                     /locus_tag="VN968_28760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HXL33261.1"
                     /translation="MLQLQSAFGVLALLALAWAFGENRGAVSLRQAAIGLAVTFVTAV
                     ALIKLPFVAHAFGVINDAVGAISAASRAGTAFVFGYLGGGALPFDLKAPGADFILAFQ
                     ALPVVLVMSVLTTLLFYWRILPPVVRGMAWLLERTLGVGGAVGLSTAANIFLGMVEAP
                     LFIRPYLAQLTRSELFLVMTGGMAGIAGTVLVLYATLLAPLIPDAAAHFVIASVLGAP
                     AAILVSLIMVPETEGKRTGGALDDPDMHAASTMDAIVKGTVAGLELLLNIVAMLIVLV
                     ALVYLANAILGLLPDVGGAGISLQRMLGYAMAPVCWLMGLSWPQAITAGSLMGVKTVL
                     NELIAYVDLSRLGSDALDPRSRLIMLYAMCGFANFGSLGIMIGGLGTMAPDRRDEINS
                     LGLKSIVSGTLTTCLMGAIVGVLS"
     gene            complement(3805..5724)
                     /locus_tag="VN968_28765"
     CDS             complement(3805..5724)
                     /locus_tag="VN968_28765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HXL33262.1"
                     /translation="MNSAYREDIDWLRAIAVLAVVAFHFEAPAVFGGFVGVDIFFVIS
                     GYLITGIIQSEVQGGTFSFAQFYERRARRLLPALYVMVALTAIPSLHYLLSSERLEFF
                     RSVAAVVTFTSNFFFWLQSGYFDHAAVEKPLLHTWSLAVEEQFYLALPVVLWVMLRFV
                     RVGRIALAVALGAMALVSFALCLWLMHTERSANAFFMSPPRAWEFLIGGLVATPGLPV
                     LRNARAQQIARGIALVLLAIPIFSLRPGPGFPGVNALLPCIGAAVFIWSGIGVATPVR
                     SPHSHHSVIRFFGRISYSLYLWHWPLFTFARFSKTSLVLDPLDKMELFALTVAISYLS
                     WRFVEQPFRLRTPAPANRGGFRMAGLATAALLAGSAGGIVLSQTPSGADRDAMMLDVY
                     NSYDVRPIYRAGSCFRYDGGAFDDARCLGAVADKQNVLLWGDSYAAQYYHGLDHTIDR
                     ERVNLLQATQAACMPTLNASAQGNASCHSFAGRMDAFFRDRKPDLVILSADWLEYARP
                     PRFEGMIADLNQTIARINATGVAVVLLGPGVQFRSRLPSMLMRAQLRHIDPQPQDFVL
                     PDIFGLDRMMKAALPAHEKFSYISVVDAVCPARQCPLTIDGGIPLVWDHAHLTAEGSV
                     YVMESMMPMLGVKKF"
     gene            complement(5783..6358)
                     /locus_tag="VN968_28770"
     CDS             complement(5783..6358)
                     /locus_tag="VN968_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXL33263.1"
                     /translation="MARKEPTKKPTSRAAARTAAQKKSPARRTAARSTARKHIKAKDR
                     KKDREAKARRLPAAKPRARPKQRIAISHYRDEDFKADGLRAYAHYRDLGIAEASHGLA
                     QAHVIRLIGPCNPAEVSKLHFHDVEFQMVYVLKGWVKTYMEGQGETLMKEGSAWTQPP
                     RIRHLIMDYSDDVELLEVILPAEFKTVELAS"
     gene            complement(6510..6794)
                     /locus_tag="VN968_28775"
     CDS             complement(6510..6794)
                     /locus_tag="VN968_28775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="usg protein"
                     /protein_id="HXL33264.1"
                     /translation="MVSRVGVSEDFRKQVLGYGLTTAQILYRMPDHPSLLQTYVWQNY
                     DLFPNFPALKDFLSFWEEKLEGPLFAVTVAHSKLIKPAELRAVDGVFRLH"
     gene            7174..>7313
                     /locus_tag="VN968_28780"
     CDS             7174..>7313
                     /locus_tag="VN968_28780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXL33265.1"
                     /translation="MKFRPLHDRVVVKRIDAEEKTAGGIIIPDSAKEKPSQGEITAVG
                     PGG"
BASE COUNT         1483 a         2263 c         2173 g         1394 t
ORIGIN      
        1 aaggctggag tgacaaccgg attatcaagg cgctggagac cagcgcatcg atggtttacc
       61 gggtccggaa gcaactggta tcagctgacc aattaaataa cttgcgtact ttcgattgga
      121 agcgaccgtg tttgatggaa agccttttct tgggacgaag ttttagagat tgccacatcg
      181 gcgaggtcat ggcggtccaa gtcggtccct caaaaggtcg atttgggggg taccgagaaa
      241 atgtgatcgt ccagcattct cctgctccca aggtcgtaat tttctaacca aactaaccgc
      301 gaaacatcga tctcgaaccg ccggcacact tgaaagcata attcttcaac gcaattcgtg
      361 atgctgttac ccggatttcc ggggacctgg atgcaaacca caacgatcct gtcatccggc
      421 aaatttattc tctcaatcag acaaattccg tctgctctat tgccggcctt gtatgcaaac
      481 aactcgggct ggtcagctac ctgccaaaga ggaaatcgcc aaggcttcga ggtgtatttg
      541 agctcatctt caaatcgttc atagacctta acgtacacag atgtttcatc ttccgaagaa
      601 gattcagaag taaggtcctc aaccgcccct tccgcagcta gtatggtggg cttagaatta
      661 atttctttgt acaactgact gcggtgttcc cgcagctcac ccggtgttat ccctttcgac
      721 tggcttgttc gactatcata ctcgtcatgg tgttcgacac atagccaaac caaattatca
      781 aaccgagagt cgttacgctt ccggttaagg tgcgcaatct gcccctttct gcggccctta
      841 tgttcacccc agcaatagca aaggcagcac ctccgtttag aaagcaccaa tacactagtc
      901 tcgatgtcct tagctggtcg ttttctcgcc accatacccg gcgcccttct gagaggcaat
      961 caaggctcaa acctttgacc gatcaagatg ccgaatcccg cttaaaattg cattaggcaa
     1021 aacggaagca aaacttccgc actaccccag ccgctccaca tcgcgcagcg tcggaaacag
     1081 cttcatccac aatagcgcga tcgcgatcgt gccgaggccg ccgagcaccg ccgccggcac
     1141 tgcgccgaac aaggcggcgg tcaccccgct ttcgaactgg ccaagctggt tggaggcgtt
     1201 gatgaacaga aaattcacgg cgccgacacg gccgcgcatt tcatcgggcg tcgcgagctg
     1261 caccagcgag aagcggatca ccacgctgac gatatcggcc gcgccaagta tcgccagcgc
     1321 cacaaccgag agccacatcc actgcgaaac ggcgaatatc accgtcgcca cgccgaacac
     1381 gatcacggcc tggaacatgc gcacgccgac gcgccggttg atggtatggc gcgcaaggac
     1441 ggcggtcatc acgagtgcgc cgaccgctgg cgcggcacgc aggatgccga ggccccaggg
     1501 gccggtctgc aggatgtcgc gcgcatagat cggcaacagc gccgtcgcgc cgcccagcag
     1561 caccgcaaac agatcgagcg agatcgttcc caggatggcg gaattcttgc ggacgaatct
     1621 gacgccggcg aacagatcgg ccggcgttgc ggaatccctg acgacggcca attgcgccag
     1681 acggatcgcg ccggtcagta tcgcccccga tagccagaac acaaccatca tgccgtaggc
     1741 catgccgggc gcgacggcat aggccagacc gccgagcgcg ggtccggtga tcgtggcgat
     1801 ttgagccgag ccgctggata aggcggtcgc gcgctgcagc gcgcccgccg gaacgatcag
     1861 cggcagcagc gcggcagttg cggggctttc gaacgcggtg gcagttccta gcacgccggt
     1921 cgcgataaag atctgcaatg ccgtcaatga gcccgaatag gcgccgcaag cgagaaataa
     1981 cgccgtcagc gcctgggcaa gctggcagat ctgcacgaca cgcttgcgct cgtagcggtc
     2041 cgcggcgtgg ccggccacga aaaccagcag cgcagtggga agaaactgca ccagcccgat
     2101 catcccgagg tcgaacgcgc tgccggtcag gtcgtagatc tgccagccga tggcaacggc
     2161 tgcgacctga ctggagaacc gcgagaaact tcgcgtgaga agaaagagca gaaaagatcg
     2221 ctgcttgagc ggcgagccct ggtctgctga tatcatggtc gatatggtca acggcgtctg
     2281 cccggttttc gaatggcggg cggtcgcccg ccgcacgtct ctggtcacgc gtggctgagc
     2341 cctgaccggt tgtcaatgtc ggggcgttcg gccgcctccc gatcgaaaac gcgcggcatg
     2401 cgccttgcag cgtcccgccg ccatcatcgc gctggcctcg ggggaatcat gctccaattg
     2461 caatcggcgt tcggcgttct tgccctgctc gcactggcgt gggcgtttgg cgagaaccgc
     2521 ggcgcggttt cgctcagaca agccgctatc ggcctcgccg tcaccttcgt tacagccgta
     2581 gcactcataa aactgccgtt cgtcgcgcat gccttcggcg tcatcaatga tgccgtcggt
     2641 gccatttcgg cggcctcgcg cgcgggcacg gccttcgtgt tcggctatct cggtggcggc
     2701 gcgctgccat tcgatctgaa agcgccgggc gcggatttta ttctcgcgtt tcaggcgctg
     2761 ccggtcgtgc tggtgatgag cgtgctcacc acgctcctgt tctactggcg tatcctgccc
     2821 ccggtggtgc gcggcatggc gtggctgctg gagcgcacgc tcggggtcgg cggcgcggtc
     2881 gggctgtcga ccgccgccaa catctttctc ggcatggtgg aagcccccct gttcatccgg
     2941 ccttatcttg cgcagctgac ccgcagcgag ttgtttctgg tgatgaccgg cggcatggcg
     3001 ggcatcgccg gcaccgtgct ggtgctctac gccaccttgc tggcgccgct gattccggat
     3061 gccgcggcgc atttcgtcat tgcctcggtt ctcggcgcgc cggcggcgat cctggtcagc
     3121 ctgatcatgg tgccggagac agaaggcaaa cgtaccggcg gcgcgctgga tgatcccgac
     3181 atgcacgccg cctccaccat ggacgcgatc gtcaagggca ccgtcgcggg cctcgaactc
     3241 ctgctcaaca tcgtcgcgat gctgatcgtg ctggtggcgc tggtgtatct cgccaacgcc
     3301 atccttggac tgttgcccga tgtcggcggc gccggaattt cactgcagcg catgctcggc
     3361 tacgccatgg cgccggtgtg ctggctgatg ggtctgtcgt ggccgcaggc gatcaccgcg
     3421 ggcagcctga tgggcgtcaa gaccgtgctc aacgagctga tcgcctatgt cgatctatca
     3481 aggcttggtt ccgatgcact cgatccgcgc tcgcggctga tcatgctcta tgcgatgtgc
     3541 ggctttgcca atttcggcag cctcggcatc atgatcggcg gcctcggcac catggcgccg
     3601 gatcgccgcg acgagatcaa ttcgctcgga ttgaaatcga tcgtctcggg caccctgacg
     3661 acctgcctga tgggtgcgat cgtgggagtg ttgagctgac ctgtcattcc ggaacgcgcc
     3721 gcttggcgcg ggcccggaat ccatactccc tgtcgtggtt atggattccg ggctcagccc
     3781 tgcaggctgc cccggaatgc caagctagaa tttcttcacc cccaacatcg gcatcatgct
     3841 ttccatcaca tacaccgacc cttccgccgt cagatgcgcg tgatcccaca ccaggggaat
     3901 gccgccgtcg atcgtgagcg ggcattgccg cgccggacag accgcatcga ccactgaaat
     3961 gtaggaaaac ttctcgtgcg ccggcagcgc tgctttcatc attcggtcga gcccgaaaat
     4021 atccggcagg acaaaatcct gaggttgcgg atcgatatgc cgcagctgcg cgcgcatcag
     4081 catcgacggc agccggctcc tgaattgcac gcctgggccg agcagcacca cggcaacgcc
     4141 cgttgcgttg atccgcgcaa tggtctggtt gagatcggcg atcatgccct cgaaccttgg
     4201 cgggcgggca tattccagcc agtcggcgga caggatgacg aggtcgggct tgcggtcgcg
     4261 gaagaatgcg tccatccgtc cggcaaagct gtggcacgac gcattgcctt gagccgacgc
     4321 gttcagcgtg ggcatgcacg ccgcttgcgt cgcctgcagc aggttgacgc gctcccgatc
     4381 aatcgtgtgg tcgaggccgt gatagtattg cgcggcataa ctgtcgcccc aaagcagcac
     4441 gttttgcttg tcggcgaccg caccaaggca gcgtgcgtcg tcaaaggcgc ctccgtcata
     4501 gcggaagcag gagcccgcgc gataaatcgg ccgcacgtcg taactgttgt agacatccag
     4561 catcatcgcg tcgcggtcgg cacctgacgg cgtctggctc aagacgattc cgccagcgct
     4621 gccggccagc agggccgccg tcgcgaggcc cgccatgcgg aacccgccgc ggtttgccgg
     4681 cgccggcgtt ctcaggcgaa acggctgttc gacaaatcgc cacgacagat atgagatcgc
     4741 aaccgtcagc gcgaacagct ccatcttgtc gagcggatcc agcaccaggc tggttttcga
     4801 gaaccgcgca aaggtgaaca gcggccagtg ccagagatac agcgaatagg agatccgccc
     4861 gaaaaacctg atgacgctgt ggtgcgaatg ggggcttcgg actggcgttg caacgccgat
     4921 gccgctccag atgaagaccg cagccccgat gcacggcaag agcgcgttga cgccgggaaa
     4981 tcccggtccc ggacgcagcg agaatatcgg gattgcgagc agcaccagcg caatgccgcg
     5041 cgcgatttgt tgcgcccgcg cgttgcgcag caccggcaag ccgggggtcg cgaccagtcc
     5101 gccgatcagg aattcccacg cccgcggcgg gctcatgaag aatgcattgg cggaacgttc
     5161 ggtatgcatc agccagaggc agagtgcaaa tgagaccagc gccatcgctc cgagcgcaac
     5221 cgccagagcg atgcgcccaa cgcgcacaaa gcgcaacatc acccacagca ccaccggcag
     5281 cgccagataa aactgctcct cgaccgccag cgaccaggtg tgtagcagcg gcttttcgac
     5341 cgcggcatga tcgaaatagc cggattgaag ccagaagaag aaattcgagg tgaaggtcac
     5401 caccgcggcg accgagcgga aaaactccag ccgctcggag ctgagcagat agtgcaagga
     5461 cggtatcgcc gtcagcgcca ccatcacgta cagcgcgggc agcaggcgtc gtgcccggcg
     5521 ctcgtagaat tgcgcaaagg agaacgttcc gccctgtact tccgactgga tgatcccggt
     5581 gatgaggtaa ccggagatca caaagaagat atcgacgccg acaaagccgc caaacactgc
     5641 cggcgcttcg aaatggaacg cgactaccgc gagcaccgcg atggcgcgca gccagtcgat
     5701 gtcctcacga taggcgctgt tcacaatggc cgggccgcct ctgaccgacg gcccgtcgcg
     5761 ctcgcaacaa cacctatgca gatcatgaag cgagttcgac cgtcttgaac tcagccggta
     5821 gaatcacctc gagcaattcg acgtcgtcgg aatagtccat gatcagatgc ctgatccggg
     5881 gcggctgggt ccatgcgctg ccctctttca tcaaggtttc gccctgccct tccatgtagg
     5941 ttttgaccca gcctttcagg acatagacca tctgaaactc gacgtcgtgg aaatgcagtt
     6001 ttgaaacctc ggccggattg cacggcccga tcagccggat cacatgcgcc tgcgccaggc
     6061 cgtggctggc ctccgcaatg ccgagatcgc gatagtgcgc gtaagcgcgc aggccatcgg
     6121 ctttgaaatc ctcgtcgcga tagtgactga tggcgatgcg ttgtttcggc cgcgcgcgcg
     6181 gctttgcggc cggcagccgc cgcgccttgg cctcgcgatc cttcttgcga tccttggcct
     6241 tgatatgttt tcgcgcggtg gatctggcag cggttctgcg cgccggcgat tttttctgcg
     6301 cggcggtgcg ggccgcggcc cgcgatgtcg gctttttcgt tggctctttc ctggccatcg
     6361 acgttctccc tttccatctc gttcggaaag gttagcacag atcgcgctgg attgggcgga
     6421 ccaagcgcca gcgggcccac cgcctggcct ccaagagagc tgtatcgggc acggcgcaag
     6481 cgcgccttga cccgctccaa gaagtccgct cagtgcagcc ggaacacgcc atcgacggcg
     6541 cgcagttcgg cgggcttgat cagcttggaa tgcgcgaccg tgacagcgaa cagcgggcct
     6601 tcgagctttt cctcccagaa ggagagaaaa tccttcagcg ccggaaaatt cggaaacaga
     6661 tcgtaatttt gccagacgta ggtttgcagc agtgaggggt gatccggcat ccgatataaa
     6721 atctgcgccg tcgtcagccc gtaacccagc acctgcttcc ggaaatcctc ggaaacccca
     6781 acccgcgaga ccatgcttac ctccattact ggagcgcatt cacgaagtgg ctgccgaccg
     6841 ggagccaccc ggtggagatg cgctcacatc agagaaatgt gacgcacgac accaactcat
     6901 cacaagccta aatgtttaat gatctgttga aaaatgtcgc gttagcagca gcttaccgca
     6961 cgtgctaata ctggggttgc gggtctgttc cgctgaatta atgatgatgg acgattttgg
     7021 caggcgtcat atttgagtgc caagttttct gctagaagcc cttgttcccc gcacaagttt
     7081 ggcctatgtc cagttcggtc gcgctggcac tcgctggcat tgactgccaa aaccaaaaat
     7141 tcgataacat cttcaaaagc ttaggaggac tgcatgaaat tccgtccgct tcacgaccgc
     7201 gttgtggtca agcgcatcga cgccgaagag aagacagctg gcggcatcat tattccggat
     7261 agtgccaagg aaaagccctc gcagggcgaa atcacggcag tcggtccggg cgg
//
LOCUS       DATNOI010000719        14327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Bradyrhizobium sp. isolate SMAG_U11527 k141_2601001,
            whole genome shotgun sequence.
ACCESSION   DATNOI010000719 DATNOI010000000
VERSION     DATNOI010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37946097
            Sequence Read Archive: SRR6266478
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bradyrhizobium sp. (soil metagenome)
  ORGANISM  Bradyrhizobium sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Nitrobacteraceae; Bradyrhizobium.
REFERENCE   1  (bases 1 to 14327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 03:49:47
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,745
            Genes (coding)                    :: 5,612
            CDSs (with protein)               :: 5,612
            Genes (RNA)                       :: 23
            tRNAs                             :: 18
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 57 of 133
            Pseudo Genes (incomplete)         :: 84 of 133
            Pseudo Genes (internal stop)      :: 8 of 133
            Pseudo Genes (multiple problems)  :: 15 of 133
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14327
                     /organism="Bradyrhizobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2601001"
                     /isolate="SMAG_U11527"
                     /isolation_source="soil"
                     /db_xref="taxon:376"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="39.7291 N 123.6419 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VN968_28785"
     CDS             complement(<1..263)
                     /locus_tag="VN968_28785"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXL33266.1"
                     /translation="MRTGKTFRERVHEAREHVAMLSPDEARQLIDLGGVLLIDVGEEW
                     QLRERGTIPGARNITRGELEIRADTEQERRDPALQDRQQKILLT"
     gene            complement(283..1056)
                     /locus_tag="VN968_28790"
     CDS             complement(283..1056)
                     /locus_tag="VN968_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HXL33267.1"
                     /translation="MNRDDPPPFVPPAEAPPEVHSGLMARFRNYFLTGLIVAGPIAIT
                     FYLTWWFVTWVDGLVRPFVPIAYRPETYLPFGLPGSGLIVAVIALTLLGFLTANLIGR
                     TLVDLGERLLGRMPVVRAIYRGLKQVFETLFSGSGSSLRRVGLVEFPSPGMWSIVLIS
                     QAPSVEIANNLPGAEEHISVFLPCAPNPTTGFFFYVPRSKIIEVDMSAEDAATLIMSA
                     GVVQPGSDPQKKIAALAGVANAARVAHESSLQPVPVKVE"
     gene            complement(1168..2994)
                     /gene="glmS"
                     /locus_tag="VN968_28795"
     CDS             complement(1168..2994)
                     /gene="glmS"
                     /locus_tag="VN968_28795"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HXL33268.1"
                     /translation="MCGIVGILGRGPVAEQLVDSLKRLEYRGYDSAGVATLEGKHLER
                     RRAEGKLKNLEARLQAEPLAGHTGIGHTRWATHGRPTENNAHPHATDRVAVVHNGIIE
                     NFRELREVLQNKGTVFKTETDTEIVLHLVDDFLKQGFTPAEAVKATLSQLRGAFALGF
                     IFAGDDDLMIGARNGPPLAIGYGEGEMYLGSDAIALGPFTDTVAYLEEGDWVVLTRTS
                     AVIYDKNNAAVRRDAIKHTGATSLVDKANYRHFMAKEIHEQPEVVGHTLARYVDMATE
                     RVALPVKLPFDFKDIQRISIVACGTASYAGYVAKYWFERLSRVPVEVDVASEFRYREA
                     PLRKGDLAIFISQSGETADTLAALRYAKGEGVHTLSVVNVASSTIARESETVLPTLAG
                     PEIGVASTKAFTCQLMVLAAIAVAAGKARGELSDVDETRLVHGLVEIPRLMAAALATE
                     PQIEKLARDIAKSKDVLYLGRGTSYPLALEGALKLKEISYIHAEGYAAGELKHGPIAL
                     IDENMPVVVIAPFDRVFEKTVSNMQEVAARGGNIILMTDAKGAAEATIESLVTIVLPD
                     MAATFTPMVYAVPVQLLAYHTAVIMGTDVDQPRNLAKSVTVE"
     gene            3304..5109
                     /locus_tag="VN968_28800"
     CDS             3304..5109
                     /locus_tag="VN968_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXL33269.1"
                     /translation="MPNAAAQVQKLLRQQAAIARFGSFALRERDLMKILTEATRVCAE
                     GLNVPFSKVCQYRAKQNDLLIVAGHGWQEGVIGYVASRADTSSPQGLAFTTGQPSICD
                     DLQKSAFKDLPPFYAAHGIVSIIDVIIKGNDIQPYGILEIDNDHQHDYDEHDINFLTG
                     FANVLAEAVLTASRGAALQVTVDRMKALVEEKDRLLDEKDVANLQLRQAQKMEAVGQL
                     TGGIAHDLNNILTVITGTIEILAEAVAGQPQLVAVAKMIDEAAARGADLTQRLLAFAR
                     KQPLQPREVDVNALVIEATNLLRPTLGEHIEVRIVLSGDTARALIDPSQLTNAILNLA
                     LNARDAMAEGGQLVIETSNVLLDDSYANVNSDVTAGNYVMIAVTDSGHGIPASILQNV
                     FEPFFTTKEVGKGSGLGLSMVYGFVKQSNGHIKIYSEKDHGTTVRIYLPLAAGAALPA
                     ADLEDTAIIEGGNETILIVEDDHLVRTFVVGQIRSLGYVTLSAVNAEEALVVIDSAQP
                     IDLLFTDMIMPGSMNGRELADEALRRRPTLKILFTSGHTENTVIHHGRLDAGVLLLAK
                     PYRKTELARMIRSAIEAEVHPPTPDRVAKRSHKAT"
     gene            complement(5116..6474)
                     /gene="glmU"
                     /locus_tag="VN968_28805"
     CDS             complement(5116..6474)
                     /gene="glmU"
                     /locus_tag="VN968_28805"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HXL33270.1"
                     /translation="MSGRTSLTIVLAAGEGARMQSSLPKVLHPVAGQSLLAHVLGAAP
                     KGAGAALAVVIGPDHGSVVEETKRLRPDATTFVQRERLGTAHAVLAARDAIARGVDDL
                     LVVFGDTPLISAETFARMRVPLKNGAALAVLGFRAADPTGYGRLVVEGDRLVAIREQA
                     EASPAERAITLCNAGVMAFDGHKALKIIEKIGNANSKGEYYLSDAVGIVRQQGLEAVV
                     IETSEDEVRGINTKAQLAEAEQVMQARLRKAALEAGVTMIAPETVYLAADTKFGSDVT
                     IEPFVVIGPGVTIADGAVIHCFSHLVQSSIGRNASVGPYARLRPGTSLGEGAKVGNFV
                     EIKAATLEAGVKVNHLSYIGDTLIGTGANIGAGTITCNYDGFGKHQTTIGSGAFVGSN
                     TSLVAPVKIGSGAYIGSGSVITRDVPDNALAVERGQQTTREGWAKRFREMKLRGKKPK
                     GG"
     gene            6652..7905
                     /locus_tag="VN968_28810"
     CDS             6652..7905
                     /locus_tag="VN968_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33271.1"
                     /translation="MAQDSDHLAGFETENTGGFLSGLLADEDHFDRRALWRLGSWGVA
                     TVGAVIVAVLAGQSSIGLHREQMASGDLARQQQIQSVAMESQNEARRLASAIETLNSD
                     RDRLYARVTVLEQGLDTVTGAIARQNSAPAWPQAATTAPATPEPPQQPAAQNPTPAPV
                     VSPVAAAPAKPAEKPPADTAPVKDSSPAAVSPVAQAPQAQASQDSPKNSAAVTPATPL
                     MASKSMLAPPDAPAGKLIPSETPVSAITASPIPEVVASAQAADVAERDPPKVAVQRTE
                     FGVDVGSANSLGGLRALWRGLLKSRSNAPLATLRPIIVVRESNSGLGMQLRLVAGPLA
                     DAAAAAKICAVMIESERTCETTVFDGQRLSMKADEVPAPVVKPPALRRRGSGKHAVQE
                     DPPKNPETSTTSTLSSMFSRVTRAQ"
     gene            8079..8459
                     /locus_tag="VN968_28815"
     CDS             8079..8459
                     /locus_tag="VN968_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33272.1"
                     /translation="MKKSPFRLTPYQVQFLLVVGFVTVGYALYLRYMAIELSTVALAC
                     DAGLQTMLCKTRILATSLFRNSVFGIVALVIATLHVIRPSIVLLTGGLIVAGFGIVLY
                     NIGLSGLALGLLILGFARPAPATA"
     gene            complement(8419..10044)
                     /locus_tag="VN968_28820"
     CDS             complement(8419..10044)
                     /locus_tag="VN968_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-(1-6) glucans synthase"
                     /protein_id="HXL33273.1"
                     /translation="MEPITFRTPLALLLISLGAIAAVWWWLATPINLARAPIDPSAKL
                     LCVSYAPFRDTQTPLMPTTQIAPEQIAQDLAQLAKISDCVRTYSLENGLDQVPALAAK
                     VGLKVILGIWLGSNRLKNLAQISTAVGLTKAYPEVITAVVVGNEVLLRGEMTTSDLAA
                     NIRSVKSQVAVPVTYADVWEYWLRNREVYEAVDFVTIHILPYWEDFPIRAKHAAAHVD
                     AIRKRMAVAFPGKEILIGETGWPSEGRMREGALPSRTNQARVVSEILGLAKQENFRVN
                     LIEAYDQPWKRQLEGTVGGYWGLIDSVQRAVKYPPGEAISNYPFWKLQMGCGMGLSFL
                     VFGAAWLTLRRRPWTPRLASWIAVGLSATTAGILLGVAADKMFYESYGFGGWLGWGAL
                     LTAAIASPLLCANATMSGRSLPTFLELLGPRDARSRSALTMILGAALVVTTLIGTETA
                     LGFVFDPRYRDFPFAALTMAVVPFSTLMLLNRRQEGVRPIAEAVFAGLLAVAALYAVF
                     NEGSDNWQSLWTCAVYFLFAVTLWRARAVQIPK"
     gene            complement(10263..10409)
                     /locus_tag="VN968_28825"
     CDS             complement(10263..10409)
                     /locus_tag="VN968_28825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXL33274.1"
                     /translation="MRKRAANTDQKCEPGDGEVAQNRKLKLKQPSTHKFPEMLPVASS
                     KVPV"
     gene            10347..10877
                     /locus_tag="VN968_28830"
     CDS             10347..10877
                     /locus_tag="VN968_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1-3, beta-1-6-glucan biosynthesis protein"
                     /protein_id="HXL33275.1"
                     /translation="MRHLAVPGLALLIGIGGALAQSASQDQSGKPVANSAASTDATKD
                     SQRKTNEFVEAAQAINGPAGNPECVWLGRRMVRLMWNDDLDTAFRHLDLYDRFGCPGG
                     HIQATFRCLTRFGGEIDSKVPESLDRHVHACWINPNAQPQAAAAAQPAAPATAGTAPA
                     TPAAAPSPAAAAPSAK"
     gene            11059..13740
                     /locus_tag="VN968_28835"
     CDS             11059..13740
                     /locus_tag="VN968_28835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXL33276.1"
                     /translation="MRAVVAVLFFVTAVHAATWGVFRDKQEAPDFKGILPSVSYAPFE
                     GTAHPDVDNIPNVEKIRADMKKLAPLTRAIRLYSSTGGVEMVPPIAAEFGIKVTLGAW
                     IDKNVDRNEREIAAAIDLAKRNSNVIGIVVGNETVFRGEQKVDGLIDLIKRVKSAING
                     RVPVTTGEIWNIWRDNPELGYSVDFIAAHVLPYWENFTDQQAVDQAVSTYQKLKDQFP
                     GKRIVIAEFGWPSAGYNLKNAVPGPFEQALVLRNFVTRAEAIGMDYNIVEAIDQPWKF
                     FEGGVGPYWGILNAAREPKFAWTGPIVNENYWKLATLALLVGVLMSLPILRLPEATVV
                     QALVLSAAANGIGAWTATVFAYWTGHYFVFGSAFALTLGLTLLVPLVLIAMARIEEIA
                     AIAFGQKPRRLLVKGQSAESAVAATAALPKVSIHVPAYFEPPEMLKQTLDAVARLDYP
                     NFECVVIINNTPDSEFWQPIQDHCRTLGERFKFINAEKVQGFKAGALRIAMERTAADA
                     EIIGVIDADYVVQPDWLKDLVPVFADPRVGLVQAPQDHRDGDRSLMHYIMNGEYAGFF
                     DIGMVQRNEANAIIVHGTMCLIRRAAMDMAGGWNGDTICEDTDLGLAIIEHGWLTHYS
                     NHRYGHGLLPDTYEAFKKQRHRWAYGGFQIVKKHWRRFLPGASRLLPDQRREFALGWL
                     NWLGAESLGVLVAILNLIWVPIVAFAGIAIPDKILTLPIIAAFVVSLVHFVVLYRLRV
                     PVKWGQMLGAMIAAMSVQWTVSRAVANGLITEHLAFARTSKGGFSLMSVEFQAFWEAV
                     IGVLLLVGAVVLVVTNNDKQVREIYVFAAVLVLESLPFLSAVAIALLENSRLNSFSFW
                     NSTGVRTLELIGLRPVAMPTVVGPSQPVATEIRRKAS"
     gene            13890..13965
                     /locus_tag="VN968_28840"
     tRNA            13890..13965
                     /locus_tag="VN968_28840"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13923..13925,aa:Lys,seq:ttt)
BASE COUNT         2697 a         4563 c         4396 g         2671 t
ORIGIN      
        1 gtcagcagga ttttctgctg ccgatcctga agtgcgggat cgcgacgttc ctgttcggta
       61 tccgccctga tttcaagctc gccccgcgtg atgttgcggg cgcccggtat agtcccccgc
      121 tcgcggagtt gccattcttc gccgacatcg atcagcagca cgccgccgag gtcgatgagc
      181 tgcctggctt cgtcaggaga gagcatcgca acgtgctcgc gcgcttcatg cacgcgttcg
      241 cgaaaagtct ttcccgtacg cattcgaggc gtcccgcaag ccttattcca ccttcaccgg
      301 caccggttgc agcgaggatt catgcgcgac gcgcgcggca ttcgccaccc ccgccagcgc
      361 ggcaatcttc ttctgcgggt cggagccggg ctgcacgacg ccggcggaca tgatcagcgt
      421 cgcggcatcc tcggcgctca tatcgacttc gatgatcttg ctcctgggca cataaaagaa
      481 aaaacccgtg gtcgggttcg gcgcgcaggg caggaacacc gagatgtgct cctccgcgcc
      541 tggcagattg ttggcgatct ccacgctcgg cgcctgcgag atcagcacga tcgaccacat
      601 ccccggcgag ggaaattcaa ccagaccgac ccggcgcagg ctcgatccgg aacccgagaa
      661 cagcgtctcg aacacctgtt tcaggccgcg atagatcgcg cgcaccaccg gcatccgccc
      721 gagcagccgc tcgccgagat cgaccagcgt ccgcccgatc agattggcgg tgaggaagcc
      781 cagcagggtc agcgcgatca cggcgacgat cagcccggaa ccgggcagcc cgaacggcag
      841 ataggtttcc ggccgatagg cgatcggaac gaacggccgc accaggccgt cgacccaggt
      901 cacgaaccac caggtcagat aaaaggtgat ggcgatcggg ccggcgacga tcaggccagt
      961 cagaaaatag ttccggaatc gggccatcaa gccgctgtgg acctccggtg gggcttcggc
     1021 cgggggcaca aacggaggcg ggtcgtcgcg gttcatgggg gttccaggtg gtcgaaatgg
     1081 ctgtcgcatc ctgacagcta agtcttgtag cagatttttg aagacttcaa gcgaccggtt
     1141 tcaggtggca tggctaccac catctgccta ttccacggtc accgatttcg cgagattgcg
     1201 cggctggtcg acgtcggtgc ccatgatgac ggcggtatga taggccaata actgcacggg
     1261 aaccgcataa accatcggcg tgaaggtcgc cgccatgtcg ggcagcacga tcgtgaccaa
     1321 ggactcgatg gtcgcttccg cggccccctt ggcgtcggtc atcagaatga tgttgccgcc
     1381 gcgggccgct acttcctgca tgttggagac ggttttctcg aacacccggt cgaacggcgc
     1441 gatcaccacc accggcatgt tctcgtcgat cagcgcgatc ggtccgtgct tgagttcgcc
     1501 ggcggcgtag ccctcggcat ggatgtagga aatttccttc agcttcagcg cgccttccag
     1561 cgccagcgga taactggtgc cgcggccgag atagagcacg tccttggatt tggcgatgtc
     1621 gcgcgccagc ttttcaattt gcggctcggt ggcgagcgct gcggccatca gtcgcgggat
     1681 ttccacgagg ccgtgcacga gcctggtttc atcgacatcg gacaattcgc cgcgcgcctt
     1741 gccggcggcc accgcgatcg cggccagcac catcagctgg caggtgaagg ctttggtcga
     1801 ggcgacgccg atttccggac cggccagcgt cggcagcacg gtctcgctct cgcgcgcgat
     1861 cgtcgaactg gcgacgttga ccaccgacag cgtgtgcacg ccttcgccct tcgcatagcg
     1921 caacgcggcc agcgtatcgg cggtctcgcc ggactgcgaa atgaagatcg ccagatcgcc
     1981 cttgcgcagc ggcgcctcgc gataacggaa ttccgaggcg acatcgactt cgacgggcac
     2041 gcggcttagg cgttcgaacc agtatttggc gacgtaaccc gcatagctcg cggtgccgca
     2101 ggcaacgatc gagatgcgct ggatgtcctt gaagtcgaac ggcagtttga cgggcagcgc
     2161 gacgcgctcg gtcgccatat cgacatagcg cgccagcgtg tggccgacca cctccggctg
     2221 ctcgtgaatt tccttggcca tgaagtggcg gtaattcgcc ttgtcgacca gtgacgtcgc
     2281 gccggtatgc ttgatcgcgt cgcgacgaac ggccgcgttg ttcttgtcgt agatgaccgc
     2341 gctggtgcgc gtcaggacga cccagtcgcc ctcctcgaga taggcgacgg tgtcggtgaa
     2401 cgggccaagc gcgatggcgt ccgagccgag atacatctcg ccttcgccat agccgatggc
     2461 tagcggcggc ccgttgcggg cgccgatcat cagatcgtcg tcgcccgcga agatgaaccc
     2521 gagtgcgaag gcgccgcgaa gctgcgacaa tgtcgccttg accgcctcgg ccggtgtgaa
     2581 gccctgcttg agaaaatcat cgaccagatg caggacgatt tcggtgtcgg tctcggtctt
     2641 gaaaaccgtg cccttgttct gcaacacctc gcgcaactcg cggaaattct cgatgatgcc
     2701 gttatggacc acggcgacac gatcggttgc atgcggatgc gcgttgttct cggtcggcct
     2761 gccgtgggtg gcccagcggg tatgtccgat gccggtatgc cccgcgagcg gttcggcttg
     2821 caaccgcgct tcgagatttt tcagtttgcc ttcggcgcgg cggcgctcga gatgcttgcc
     2881 ctccagggtg gcgacccccg cggaatcgta accgcggtat tcgagtcgct tgagcgaatc
     2941 caccaactgc tccgcgaccg gaccgcgtcc gagaatgccg acaatgccgc acatgcggat
     3001 caatatctcc agatcgtcga aaattcctag atcagtgcgt ccatctctta acgaaaatcg
     3061 cgggtagcca gatactcaat aattattgcc gattgcgaca gggttaaacc gaaagcttaa
     3121 cagcgctggg cgccgatgtc ccaggaaccg acggggtcag gaagcccggc gccatgatga
     3181 cctgaattgc tcatcctcta tcatgaattg aatgtacggt aacttgacga ttcgtgttcc
     3241 ggtcccgaca tcaattgtct cgtcgatcct gccctccctc catttggacc tgacaatcac
     3301 aggatgccca acgccgccgc acaagtccaa aagctgcttc gccaacaggc ggccatcgcc
     3361 aggttcggca gtttcgcgct gcgcgaacgg gacctgatga agatcctgac cgaagcgacg
     3421 cgggtctgcg ccgaaggcct gaacgttccc ttcagcaagg tctgccaata ccgcgccaag
     3481 cagaacgatc ttctgatcgt ggccggccat ggctggcagg aaggcgtgat cggttatgtg
     3541 gcgtcgcgcg ccgatacgag ttccccgcag gggctcgcct tcaccaccgg ccagccctcg
     3601 atctgcgacg atttgcaaaa gagcgctttt aaggacctgc ctccgtttta tgccgcgcac
     3661 ggcatcgtct cgatcatcga cgtcatcatc aagggcaatg acatccagcc ttacggaata
     3721 ctcgagatcg acaacgatca tcaacacgat tacgacgagc atgacatcaa ttttctcacg
     3781 ggcttcgcca acgtgctggc ggaggcggtc ttgaccgcct cgcgcggcgc ggcgctgcag
     3841 gtcacagtcg atcgaatgaa ggcccttgtc gaggaaaagg accgtctgct ggacgaaaag
     3901 gacgtcgcca atctgcagct tcgtcaggcg cagaaaatgg aggcggtcgg gcagctgacc
     3961 ggcggcatcg cccacgatct gaacaacatt ctgaccgtca tcaccgggac catcgaaatt
     4021 ctggccgagg cggttgccgg tcagccgcaa ctcgtcgcgg tcgcgaagat gatcgatgaa
     4081 gcggccgcgc gaggtgccga tctgacccag cgcctgctgg cgttcgcgcg caagcagccg
     4141 ctgcaaccgc gcgaagtcga tgtcaatgcg ctggtcatcg aagcaaccaa cctgctgcgc
     4201 ccgacgctcg gcgaacacat cgaggtccgg atcgtgctgt ccggagacac cgcacgcgcc
     4261 ctgatcgacc ccagccagct caccaacgcg atactcaatc tagcgctcaa cgctcgcgac
     4321 gcgatggcgg aaggcggcca actcgtcatc gaaaccagca atgtcctgct cgacgacagc
     4381 tacgccaacg tcaacagcga tgtcactgcc ggaaattacg tgatgattgc ggtgaccgat
     4441 tccggccacg gcattcccgc cagcatcctg caaaatgtct tcgagccctt cttcaccacc
     4501 aaggaagtcg gcaaggggtc cggcctgggc ctcagcatgg tctacggctt cgtcaagcag
     4561 tccaacgggc atatcaagat ctacagcgaa aaggaccacg gcacgacggt gagaatctat
     4621 ctgccgctag cggccggcgc cgccttgcct gcggcggatc tggaagatac tgcaattatc
     4681 gaaggcggca atgagacgat cctgatcgtc gaggacgatc atttggtgcg aaccttcgtg
     4741 gtcggtcaaa tccggagcct gggttatgtc acgctgtcgg cggtcaacgc ggaggaagcc
     4801 cttgtcgtca tcgacagcgc ccagccaatc gacctcttgt tcaccgacat gatcatgccg
     4861 gggtcgatga acggccgcga actcgccgac gaagccttgc gacgccggcc aacgctgaaa
     4921 atcctgttca cctcgggcca tacggaaaat accgtcatcc accacggccg tctcgatgcc
     4981 ggtgtcctgc tgctggcaaa accctatcgc aagacggagc ttgcgcgcat gatacgaagc
     5041 gcaatcgagg ccgaagttca cccgccgacg ccggaccggg ttgcgaagcg ttcgcacaaa
     5101 gcaacttgac cgccgtcagc cgcccttcgg cttcttgccg cgcaatttca tctcgcgaaa
     5161 tcgcttggcc cagccctcgc gcgtggtctg ctgaccgcgt tccaccgcaa gcgcattgtc
     5221 gggcacgtcg cgggtgatca ccgaccccga tccgatatag gcgccgctgc cgatcttgac
     5281 cggcgcgacc agcgaggtat tcgagccgac gaacgcgccc gatccgatcg tggtctggtg
     5341 cttgccgaag ccgtcatagt tgcaggtgat ggtgccggcg ccgatgttgg cgccggtgcc
     5401 gataagggtg tcgccgatat agctgagatg attgaccttg acgccggctt ccagcgtcgc
     5461 ggctttgatc tcgacgaaat tgccgacctt ggcgccctcg cccagtgagg ttcccggccg
     5521 cagccgcgca taggggccga ccgacgcatt ccgcccgatc gatgactgca cgaggtgcga
     5581 aaagcaatgg atcaccgcgc cgtcggcgat cgtcacgccg ggaccgatca ccacaaacgg
     5641 ctcgatcgtg acgtcgctgc cgaatttggt atcggcggcg aggtagaccg tctcgggcgc
     5701 gatcatggtc acgccggcct caagtgccgc cttgcgcagc cgcgcctgca tcacctgctc
     5761 ggcttcggcg agctgggcct tggtgttgat gccacgcact tcatcctcgc tggtttcgat
     5821 cacgacggcc tctagtccct gttgacgaac gatgccgacc gcatcggaga gataatactc
     5881 gcccttgctg ttggcgttac cgattttttc gatgatcttc agcgccttgt ggccgtcaaa
     5941 cgccatcacg ccggcgttgc agagcgtgat cgcgcgctcg gcgggactgg cctcggcctg
     6001 ctcgcggatc gccaccaacc gatcgccctc gaccacgagc ctgccgtagc cggtgggatc
     6061 ggcggcgcga aaaccgagca cggccagcgc cgcgccgttt ttcaacggca cccgcatccg
     6121 cgcaaaggtc tcggccgaaa tcagcggcgt atcgccgaac acgaccagca ggtcgtcgac
     6181 gccgcgggcg atggcgtcgc gggccgccag caccgcatgc gcggtgccga gacgctcgcg
     6241 ctgaacaaat gtcgttgcat ccggacgcaa acgttttgtc tcttccacga cagaaccgtg
     6301 gtcgggtcca atgacaacgg cgagcgcggc gcctgcgccc tttggagcgg cccccagcac
     6361 atgcgccagc agcgattggc cggcgacggg atgcagcacc ttcggcagcg aggattgcat
     6421 gcgggcgccc tcaccggcgg caagcacgat cgtgaggctg gttctgccgg acatgtcgat
     6481 tccctgtcag gcgcgacgaa gttgcgccgc cgtcgtcata aaacgttttc ttctgccatg
     6541 gaaaccggtt tcgtcacgcg ggaaaacgcg gcgaattcaa gaccacggtg cgattcctgt
     6601 taatgtttgc ctctgattcg gctgaggcct cgggagggcc gagaacgtcc tatggcacag
     6661 gattccgacc atctggccgg cttcgaaacc gaaaacaccg gcggattcct gagcggtctt
     6721 ttggccgacg aggatcattt cgaccgccgc gcgctgtggc ggctcggatc gtggggcgtt
     6781 gccacggtcg gcgctgtcat cgtcgcggtg ctcgccggcc agtcatcgat cggattgcac
     6841 cgggaacaaa tggcgtcggg cgatctggcg cgccagcagc aaatccagtc cgtcgccatg
     6901 gaaagccaga acgaggcgcg gcggctggcc tccgccatcg aaacgctcaa cagcgaccgc
     6961 gaccggttgt acgcgcgtgt caccgttctg gaacagggac tggatacggt gaccggcgcg
     7021 attgcgcggc aaaattcggc cccggcatgg ccacaagccg ctacgaccgc gccggcgaca
     7081 cccgagccgc cccagcagcc ggcggcgcaa aatccgaccc ctgctccggt cgtgtctccg
     7141 gtggcggctg cgccagcgaa gccagccgaa aagccgccgg cggacacagc ccctgtgaag
     7201 gattcaagtc cggccgcggt ctcgccggtc gcccaggccc cccaggcgca ggcctcccaa
     7261 gactccccca aaaattctgc tgcggtgacg ccggccacac cgctgatggc ctctaaatca
     7321 atgttggctc cgccggatgc gccggccggg aaattgatcc catccgagac gccggtgagc
     7381 gcgatcaccg cttcgccgat accggaggtg gttgcgtcgg cgcaagctgc ggacgtagcg
     7441 gaacgggatc cccccaaggt cgcggtccag cgaaccgaat tcggggtgga tgtgggcagc
     7501 gccaattcgc tcggcggcct gcgcgcgctg tggcggggcc ttctcaagtc gaggtcgaat
     7561 gcgccgctgg cgacattgcg cccgatcatc gtggtgaggg aaagcaacag cgggctgggc
     7621 atgcagttgc ggctggttgc cggaccgctc gccgatgccg cggcggcggc gaaaatctgt
     7681 gccgtcatga tcgaaagcga gcggacctgc gagaccacgg tgttcgatgg ccagcgtctt
     7741 tccatgaagg ccgacgaggt gcccgcgccc gtcgtaaagc ctcccgcatt gcgcaggcgg
     7801 ggcagcggaa agcacgcggt gcaggaggac ccgccgaaaa atccggaaac ctccacaacc
     7861 tccacgttgt cgtcgatgtt cagcagggtc acgcgagccc agtaaagctc gtcatcgcaa
     7921 gaccttgggg cggtttttca gtgctttttc tcggtcgctt cgaagaacct gcccgcgccg
     7981 ggttgtcccg ccgcgcattc ccgaccatat tcacaactat tggtcagacg cattttcctg
     8041 cggctaaccg gattccactt agccggaaaa atgctctcgt gaaaaaatct ccattccggc
     8101 tgacgcccta tcaggtgcaa tttctgttgg tcgtcggctt cgttaccgtc ggttacgccc
     8161 tctatctgcg ttatatggcg atcgagctct cgacggtggc gctggcctgc gatgccggcc
     8221 tgcagaccat gctgtgcaag acccgaatcc tcgcgacctc tctgttcagg aattcggtgt
     8281 tcggcattgt cgcgcttgtt atcgcgacgc ttcacgtgat ccggccgtcg atcgtcctgc
     8341 tcacgggcgg gctgatcgtc gccggattcg ggatcgtgct ctacaatatc ggcctgtcgg
     8401 gtctggcact cggcctgctt attttgggat ttgcacggcc cgcgcccgcc acagcgtaac
     8461 cgcgaacaga aaatacaccg cgcaggtcca cagcgattgc cagttgtccg agccttcgtt
     8521 gaacaccgcg tagagcgcgg caaccgccag caggccggcg aatacggcct cggcgatcgg
     8581 ccgcacgccc tcctgccgtc ggttgagcag catcagggtc gaaaacggca ccacggccat
     8641 ggtcagcgcg gcaaagggaa aatcgcggta gcgcggatcg aacacaaagc cgagcgcggt
     8701 ctcagtgccg atcaacgtgg tcacgacgag tgctgcgccc aggatcatcg tcagcgccga
     8761 tcgcgatcgc gcatcgcggg ggcccagcag ttccagaaaa gtcggcaagc tccggcctga
     8821 cattgttgcg ttggcgcaaa gcaggggcga agcgatcgcg gccgtcagca acgcgcccca
     8881 tccgagccag ccgccgaacc cgtagctttc atagaacatc ttgtcggcgg cgacgcccag
     8941 caggattccg gccgtggtcg ccgatagtcc gaccgcgatc caggatgcga ggcgcggcgt
     9001 ccacggccgc cggcgcaggg tcagccaggc cgcgccgaac accaaaaagc tcaagcccat
     9061 gccgcatccc atctgcagct tccagaacgg atagttgctg atggcttcgc cgggcggata
     9121 tttcaccgca cgctgcaccg aatcgatcag gccccaatat ccgccgaccg tgccctcgag
     9181 ctggcgcttc cacggctggt cgtaagcctc gatcagattg acgcgaaaat tctcctgctt
     9241 cgccaacccc agaatttccg agaccacccg cgcctggttg gtccgcgacg gcagcgcccc
     9301 ctcgcgcatc cgcccctcgc ttggccagcc agtctcgccg atcaggattt ccttgcccgg
     9361 aaacgccacc gccatccgct tgcgaatggc atcgacatgg gcggcggcat gtttggcgcg
     9421 gatcggaaag tcttcccagt agggaaggat atgaatcgtg acaaaatcca ccgcttcata
     9481 gacttcccgg ttgcgaagcc aatattccca gacgtcggca taggtgaccg gaaccgcaac
     9541 ttgcgatttg accgagcgaa tgttggcggc gaggtcggac gtggtcatct caccgcgcaa
     9601 cagcacttcg ttaccgacca cgacggctgt aatgacctcg gggtatgcct tggtcaggcc
     9661 gaccgcggtc gaaatctgcg cgaggttctt cagacggtta ctgccgagcc agatcccgag
     9721 gatgactttc agcccgactt ttgcggcgag tgcaggaacc tgatcgaggc cgttttccag
     9781 cgaataggtg cgcacacaat cggatatttt ggcaagttgc gccagatcct gggcaatctg
     9841 ttccggggcg atctgcgtgg tcggcatcag cggcgtctgg gtgtcacgga acggcgcata
     9901 ggacacgcac aacagcttgg cgctgggatc gatcggcgcc cgcgccagat tgataggcgt
     9961 ggccagccac caccacacgg cagcaatcgc acccagggag atcaggagaa gcgccagtgg
    10021 cgtacgaaaa gtaatcggtt ccatcctccg ggcgggggcc cgtcgattag ccggtcacac
    10081 accgtctgcc aagagcggca gtgcataatc cttgccgtca ttatccttgc cgtcccaccc
    10141 cgcggcttta ccacactgcg ataaagttgt gactttgctg gacaaaatcg gaaataccgg
    10201 tcatgggagt tgcttgggag ttcgcagtgt ctccgccgca atggggaccc atttgaacgt
    10261 catcaaaccg gcacctttga gctggccaca ggcaacattt cggggaattt atgggtcgac
    10321 ggctgcttca gtttcaactt gcggttttgc gccacctcgc cgtccccggg ctcgcacttc
    10381 tgatcggtat tggcggcgcg cttgcgcaaa gcgcctccca ggatcagtcg ggcaaaccgg
    10441 tggcaaattc ggccgcatcc accgatgcga ccaaggacag ccagcgtaaa accaacgaat
    10501 tcgtcgaagc ggcgcaggcc atcaacggtc cggccggtaa cccggaatgc gtgtggctcg
    10561 gtcgccgcat ggtgcggctg atgtggaacg acgatctcga cactgccttt cggcatctcg
    10621 atctctatga tcgttttggc tgtcccggag gtcacatcca ggcgacgttc cgttgcctga
    10681 cccgcttcgg cggcgaaatc gactccaagg tgccggagtc gctggaccgc catgttcacg
    10741 cctgctggat caatcccaat gcccagcccc aagccgccgc ggctgcgcag ccggcggcgc
    10801 cggccaccgc cggcacggcg cccgcgacac ccgccgcggc accctcgccg gccgccgccg
    10861 cgccctccgc caaataatct gattgccacg gaacaatccg aaatttgtgc ggtttggatt
    10921 tctgcgccct gaaaacgcca cggcgtcata acagttgtta aatcgcgtgg cagagatatg
    10981 ctcgatcagc cgctgacttg gtcggatctt ggggacggat ccgctgccct gccatcttga
    11041 cccctttatg gtttagcgat gcgcgccgtc gtcgccgttc tgttttttgt gaccgccgtt
    11101 cacgctgcca cgtggggagt gttccgggac aagcaggagg ctcctgactt caagggtatc
    11161 ctgccgagcg tgtcctatgc gccgttcgag ggcacggcgc atcccgacgt cgacaatatt
    11221 cccaacgtcg agaaaatccg cgccgacatg aaaaagctgg cgccgctgac gcgggcgatc
    11281 cggctgtact cctcgaccgg tggggtggaa atggtgccgc cgatcgccgc ggaattcggc
    11341 atcaaggtca cgctcggcgc gtggatcgac aagaacgtcg accgcaatga gcgcgaaatc
    11401 gccgcggcca tcgatcttgc caagcgcaac agcaatgtga tcggcatcgt ggtcggcaac
    11461 gagaccgtgt ttcgcggcga gcaaaaggtc gatggcctga tcgacctgat caagcgggtc
    11521 aagtcagcga tcaatggccg ggtgcctgtc acgaccggcg aaatctggaa catatggcgc
    11581 gacaatccgg aactcggtta ttccgtcgat ttcattgccg ctcacgtctt gccgtactgg
    11641 gaaaacttca ccgaccagca ggcggtggat caggcagttt cgacatatca gaagctgaag
    11701 gatcaatttc ccggcaagcg gatcgtgatc gcggaattcg gctggcctag cgccggttac
    11761 aatttgaaaa atgccgttcc cggtccgttc gagcaggctc tggtgctgcg caactttgtc
    11821 acccgcgccg aggcgatcgg catggattac aacatcgtcg aggcgatcga tcagccctgg
    11881 aagttctttg aaggcggcgt cgggccctat tggggcattc tcaacgcggc ccgcgagccg
    11941 aaatttgcct ggaccgggcc gatcgtcaac gagaactact ggaaactcgc caccctcgct
    12001 ttgctggtcg gcgtgttgat gtcgctgccg atcctgcgcc tccccgaggc caccgtggtg
    12061 caggcgctgg tgctgtcggc ggcggcgaac ggcatcgggg catggaccgc gacggtgttt
    12121 gcatactgga ccggccacta cttcgtgttc ggctcggcct ttgcgctgac gctgggacta
    12181 accctgctgg ttccgctggt gctgatcgcg atggcacgga tcgaggaaat cgccgccatt
    12241 gcattcggcc aaaagccgcg ccggctgctg gtaaaggggc agtcggccga gtcggccgtt
    12301 gccgctaccg cggcgcttcc aaaggtttcg atccatgtgc cggcctattt cgagccgccc
    12361 gagatgctca agcagacgct cgatgccgtg gcgcggctgg attacccgaa tttcgaatgc
    12421 gtcgtgatca tcaacaacac gccggattcg gaattctggc agccgatcca ggatcactgc
    12481 cgcacactcg gtgagcgttt caagttcatc aatgccgaga aggtgcaggg tttcaaggcc
    12541 ggcgcgctgc gcatcgccat ggagcgcacc gccgcggatg ccgagatcat cggcgtcatc
    12601 gacgccgact atgtcgtgca gccggactgg ctgaaggatc tggtgccggt gttcgccgat
    12661 ccccgcgtcg gcctggtgca ggcgccgcag gaccatcgcg acggcgaccg ctcgctgatg
    12721 cactacatca tgaacggcga atatgccggg ttcttcgaca tcggcatggt ccagcgcaac
    12781 gaagccaacg ccatcatcgt ccacggcacc atgtgcctga tccggcgcgc cgcgatggac
    12841 atggccggcg gttggaacgg cgacaccatc tgtgaggaca ccgaccttgg cctcgccatc
    12901 atcgagcacg gctggctgac ccactactcc aaccaccgct acggccacgg cctgctgccg
    12961 gacacctatg aggccttcaa gaagcagcgc caccgctggg cctatggcgg gttccagatc
    13021 gtcaagaaac actggcggcg ttttctgccc ggcgcgagcc ggctgttgcc ggatcagcgg
    13081 cgcgaattcg cactcggctg gctgaactgg ctcggcgccg aaagtctcgg cgtgctggtc
    13141 gccatcctca atctgatctg ggtgccgatc gtggcgttcg ccggcatcgc cattcccgac
    13201 aagatcctga cgttgccgat catcgccgcc tttgtggtct cgctggtgca tttcgtggtg
    13261 ctgtaccgtc tgcgcgtgcc ggtcaaatgg ggacagatgc tgggcgccat gattgccgcg
    13321 atgtcggtgc aatggacggt gtcgcgcgcg gtggcgaacg gactgatcac cgagcacctc
    13381 gccttcgcgc gcacctccaa gggcggattt tcgctgatgt cggtcgaatt ccaggcattc
    13441 tgggaggccg tcatcggcgt cctgctgctg gtcggcgcgg tcgtgctggt ggtcaccaac
    13501 aacgacaagc aggtccgcga gatctacgtg ttcgcggccg tgctggtgct ggaaagcctg
    13561 ccgttcctgt cggcggtggc catcgcgctg ctggaaaact cgagactcaa ctcgttctcg
    13621 ttctggaaca gcaccggcgt gcggaccctg gagttgatcg ggctgcgccc ggtggcgatg
    13681 ccgaccgtgg tcggcccctc ccagccggtg gccaccgaga ttcgccgcaa agccagttga
    13741 ccaagacggg cttttgcccg ccgcatgcgg gtgaaggctc ggcgctaaca gccggggcga
    13801 ccacggaaat ccccgcggta tcgtcggccc gttgacggtt tgcgcgctat tgacccgcac
    13861 caagccgccc cctacacagc cggctaagtg agcgcgtagc tcaggtggta gagcacgtga
    13921 cttttaatca tggggtcgag ggttcgagtc cctccgcgct caccatgaaa atcaaacact
    13981 tagctaaaat tcgaggatcc cggcaaaagc cgcgtgttgg cgctgtgttg gcaaatcgac
    14041 tgccgcgccg ccttggccta ttcgaacaga cacggattgc aggatgacct ctccgtgact
    14101 ctaggaaggt cgccagtgag tcccggattt tgtggctgtc ggatgaagac ggcccggctt
    14161 aaacgaatcg ggccgcccgg cgtcggagcg aggcggccct gcacgaccgc atggtgtgat
    14221 cagatttcaa caccggcgga aaatcaactg cggttcaccg tcccaagcac tgacaccgta
    14281 cttctccggc tttttttggt gtcgacgaat cggcatgatg ccgaccg
//
